bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the risk of knee osteoarthritis following unilateral ACL reconstruction based on an EMG-assisted method
Authors: Long Ting , Fernandez Justin , Liu Hui , Li Hanjun
Published: April 2023

Objective: Anterior cruciate ligament reconstruction (ACLR) cannot decrease the risk of knee osteoarthritis after anterior cruciate ligament rupture, and tibial contact force is associated with the development of knee osteoarthritis. The purpose of this study was to compare the difference in bilateral tibial contact force for patients with unilateral ACLR during walking and jogging based on an EMG-assisted method in order to evaluate the risk of knee osteoarthritis following unilateral ACLR.

Methods: Seven unilateral ACLR patients participated in experiments. The 14-camera motion capture system, 3-Dimension force plate, and wireless EMG test system were used to collect the participants’ kinematics, kinetics, and EMG data during walking and jogging. A personalized neuromusculoskeletal model was established by combining scaling and calibration optimization. The inverse kinematics and inverse dynamics algorithms were used to calculate the joint angle and joint net moment. The EMG-assisted model was used to calculate the muscle force. On this basis, the contact force of the knee joint was analyzed, and the tibial contact force was obtained. The paired sample t-test was used to analyze the difference between the participants’ healthy and surgical sides of the participants.

Results: During jogging, the peak tibial compression force on the healthy side was higher than on the surgical side (p = 0.039). At the peak moment of tibial compression force, the muscle force of the rectus femoris (p = 0.035) and vastus medialis (p = 0.036) on the healthy side was significantly higher than that on the surgical side; the knee flexion (p = 0.042) and ankle dorsiflexion (p = 0.046) angle on the healthy side was higher than that on the surgical side. There was no significant difference in the first (p = 0.122) and second (p = 0.445) peak tibial compression forces during walking between the healthy and surgical sides.

Conclusion: Patients with unilateral ACLR showed smaller tibial compression force on the surgical side than on the healthy side during jogging. The main reason for this may be the insufficient exertion of the rectus femoris and vastus medialis.
Effects of cadmium exposure during the breeding period on development and reproductive functions in rare minnow (Gobiocypris rarus)
Authors: Su Liangxia , Li Huanhuan , Qiu Ning , Wu Yinrui , Hu Bing , Wang Rui , Liu Jun , Wang Jianwei
Published: April 2023

Cadmium is a common reproductive toxin in aquatic systems. Cd exposure of fish species at high concentrations can severely affect the reproductive function of fish. However, the underlying toxicity of cadmium exposure at low concentrations on the reproductive function in parental fish remains unclear. To investigate the impacts of cadmium exposure on reproductive capability, eighty-one male and eighty-one female rare minnows (Gobiocypris rarus) were exposed to cadmium at 0 (control group), 5 and 10 μg/L for 28 days, and then transferred into clean water to pair spawn. The results showed that cadmium exposure at 5 or 10 μg/L for 28 days in rare minnows could reduce the success rates of pair spawning in parent rare minnows, lessen no-spawning activities, and prolong the time for first spawning. Furthermore, the mean egg production of the cadmium exposure group increased. The fertility rate of the control group was significantly higher than that of the 5 μg/L cadmium exposure group. Anatomical and histological data further revealed that the intensity of atretic vitellogenic follicles significantly increased and spermatozoa vacuolated after cadmium exposure (p< 0.05), but slightly increased the condition factor (CF), and relatively stable gonadosomatic index (GSI) values were also observed in the cadmium exposure groups. These observed results indicated that cadmium exposure at 5 or 10 μg/L affected the reproductive activity of paired rare minnow by accumulating Cd in the gonads, and the effect diminished over time. The reproductive risk of low-dose cadmium exposure to fish species remains a cause for concern.
Root architecture and visualization model of cotton group with different planting spacing under local irrigation
Authors: Gao Chao , Li Mingsi , Li Dongwei
Published: April 2023

Planting spacing plays a key role in the root system architecture of the cotton group under local irrigation. This study used the Cellular Automata (CA) theory to establish a root visualization model for the cotton group at two different planting spacing (30 and 15 cm) within a leaching-pond. At a planting spacing of 30 cm, the lateral roots grew almost horizontally toward the irrigation point, and a logarithmic relationship was observed between root length density and soil water suction. However, at a planting spacing of 15 cm, the lateral roots exhibited overlapping growth and mainly competed for resources, and a power function relationship was observed between root length density and soil water suction. The main parameters of the visualization model for each treatment were essentially consistent with the experimental observations, with respective simulation errors were 6.03 and 15.04%. The findings suggest that the correlation between root length density and soil water suction in the cotton plants is a crucial driving force for the model, leading to a more accurate replication of the root structure development pathway. In conclusion, the root system exhibits a certain degree of self-similarity, which extends into the soil.
iReenCAM: automated imaging system for kinetic analysis of photosynthetic pigment biosynthesis at high spatiotemporal resolution during early deetiolation
Authors: Balakhonova Veronika , Dobisova Tereza , Benedikty Zuzana , Panzarova Klara , Pytela Jaromir , Koci Radka , Spyroglou Ioannis , Kovacova Ingrid , Arnaud Dominique , Skalak Jan , Trtilek Martin , Hejatko Jan
Published: April 2023

Seedling de-etiolation is one of the key stages of the plant life cycle, characterized by a strong rearrangement of the plant development and metabolism. The conversion of dark accumulated protochlorophyllide to chlorophyll in etioplasts of de-etiolating plants is taking place in order of ns to µs after seedlings illumination, leading to detectable increase of chlorophyll levels in order of minutes after de-etiolation initiation. The highly complex chlorophyll biosynthesis integrates number of regulatory events including light and hormonal signaling, thus making de-etiolation an ideal model to study the underlying molecular mechanisms. Here we introduce the iReenCAM, a novel tool designed for non-invasive fluorescence-based quantitation of early stages of chlorophyll biosynthesis during de-etiolation with high spatial and temporal resolution. iReenCAM comprises customized HW configuration and optimized SW packages, allowing synchronized automated measurement and analysis of the acquired fluorescence image data. Using the system and carefully optimized protocol, we show tight correlation between the iReenCAM monitored fluorescence and HPLC measured chlorophyll accumulation during first 4h of seedling de-etiolation in wild type Arabidopsis and mutants with disturbed chlorophyll biosynthesis. Using the approach, we demonstrate negative effect of exogenously applied cytokinins and ethylene on chlorophyll biosynthesis during early de-etiolation. Accordingly, we identify type-B response regulators, the cytokinin-responsive transcriptional activators ARR1 and ARR12 as negative regulators of early chlorophyll biosynthesis, while contrasting response was observed in case of EIN2 and EIN3, the components of canonical ethylene signaling cascade. Knowing that, we propose the use of iReenCAM as a new phenotyping tool, suitable for quantitative and robust characterization of the highly dynamic response of seedling de-etiolation.
Recovery of the soil fungal microbiome after steam disinfection to manage the plant pathogen Fusarium solani
Authors: Larson Eric R. , Crandall Sharifa G.
Published: April 2023

Soil disinfection using high temperatures via steam is a promising approach to manage plant pathogens, pests, and weeds. Soil steaming is a viable option for growers who are moving away from dependence on chemical soil fumigants, especially in plant nursery or high tunnel environments. However, there are few studies that investigate how soil steaming causes substantial disturbance to the soil by killing both target pathogens and other soil biota. Steaming treatments also change the trajectory of the soil microbiome as it reassembles over time. Growers are interested in the health of soils after using steam-disinfection, especially if a virulent pathogen colonizes the soil and then flourishes in a situation where there are very few microbes to suppress its growth. Should recruitment of a virulent pathogen occur in the soil, this could have devasting effects on seed germination, seedling establishment and survival. Beneficial microbes are often used to prevent the colonization of plant pathogens, especially after a soil-steaming event. Here, we experimentally test how soil fungal communities assemble after steaming disinfection. We introduce to steam-treated soil Fusarium solani, an important fungal pathogen of soybean and Trichoderma harzianum, a known beneficial fungus used for soilborne pathogen suppression. Results show that F. solani significantly affects the relative abundance and diversity of the soil fungal microbiome, however, T. harzianum does not mitigate the amount of F. solani in the steam treated soil. Within the T. harzianum microbial addition, the soil fungal communities were similar to the control (steaming only). This result suggests inoculating the soil with T. harzianum does not drastically alter the assembly trajectory of the soil fungal microbiome. Other soil amendments such as a combination of Trichoderma spp. or other genera could suppress F. solani growth and shift soil microbiome composition and function post-steaming, however, more experimental research is needed.
Vis-NIR and SWIR hyperspectral imaging method to detect bruises in pomegranate fruit
Authors: Okere Emmanuel Ekene , Ambaw Alemayehu , Perold Willem Jacobus , Opara Umezuruike Linus
Published: April 2023

Introduction

Fresh pomegranate fruit is susceptible to bruising, a common type of mechanical damage during harvest and at all stages of postharvest handling. Accurate and early detection of such damages in pomegranate fruit plays an important role in fruit grading. This study investigated the detection of bruises in fresh pomegranate fruit using hyperspectral imaging technique.

Methods

A total of 90 sample of pomegranate fruit were divided into three groups of 30 samples, each representing purposefully induced pre-scanning bruise by dropping samples from 100 cm and 60 cm height on a metal surface. The control has no pre-scanning bruise (no drop). Two hyperspectral imaging setups were examined: visible and near infrared (400 to 1000 nm) and short wavelength infrared (1000 to 2500 nm). Region of interest (ROI) averaged reflectance spectra was implemented to reduce the image data. For all hypercubes a principal components analysis (PCA) based background removal were done prior to segmenting the region of interest (ROI) using the Evince® multi-variate analysis software 2.4.0. Then the average spectrum of the ROI of each sample was computed and transferred to the MATLAB 2022a (The MathWorks, Inc., Mass., USA) for classification. A two-layer feed-forward artificial neural network (ANN) is used for classification.

Results and discussion

The accuracy of bruise severity classification ranged from 80 to 96.7%. When samples from both bruise severity (Bruise damage induced from a 100cm and 60 cm drop heights respectively) cases were merged, class recognition accuracy were 88.9% and 74.4% for the SWIR and Vis-NIR, respectively. This study implemented the method of selecting out informative bands and disregarding the redundant ones to decreases the data size and dimension. The study developed a more compact classification model by the data dimensionality reduction method. This study demonstrated the potential of using hyperspectral imaging technology in sensing and classification of bruise severity in pomegranate fruit. This work provides the foundation to build a compact and fast multispectral imaging-based device for practical farm and packhouse applications.
Cutaneous tuberculosis—ambiguous transmission, bacterial diversity with biofilm formation in humoral abnormality: case report illustration
Authors: Zdziarski Przemysław , Paściak Mariola , Chudzik Anna , Kozińska Monika , Augustynowicz-Kopeć Ewa , Gamian Andrzej
Published: April 2023

Background

Cutaneous tuberculosis (CTB) and its paucibacillary forms are rare and difficult to diagnose, especially in immunocompromised patients with significant comorbidity. The aim of the study was to introduce the modern concept of the microbiome and diagnostic chain into clinical practice (patient-centered care) with the presentation of an atypical form of cutaneous tuberculosis with necrotizing non-healing ulcers leading to polymicrobial infection.

Methods

The study material included samples from sputum, broncho-alveolar lavage and skin ulcer, taken from a patient developing cutaneous tuberculosis. The microbiological investigation was performed, and identification of the isolates was carried out using genotyping and the matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Results

The immunocompromised patient with humoral abnormality (plasma cell dyscrasia) and severe paraproteinemia developed multiorgan tuberculosis. Although cutaneous manifestation preceded systemic and pulmonary symptoms (approximately half a year), the mycobacterial genotyping confirmed the same MTB strain existence in skin ulcers and the respiratory system. Therefore, the infectious chain: transmission, the portal of entry, and bacterial spreading in vivo, were unclear. Microbial diversity found in wound microbiota (among others Gordonia bronchialis, Corynebacterium tuberculostearicum, Staphylococcus haemolyticus, and Pseudomonas oryzihabitans) was associated with the spread of a skin lesion. The in vitro biofilm-forming capacity of strains isolated from the wound may represent the potential virulence of these strains. Thus, the role of polymicrobial biofilm may be crucial in ulcer formation and CTB manifestation.

Conclusions

Severe wound healing as a unique biofilm-forming niche should be tested for Mycobacterium (on species and strain levels) and coexisting microorganisms using a wide range of microbiological techniques. In immunodeficient patients with non-typical CTB presentation, the chain of transmission and MTB spread is still an open issue for further research.
Geographic heterogeneity of the epidemiological impact of the COVID-19 pandemic in Italy using a socioeconomic proxy-based classification of the national territory
Authors: Petrelli Alessio , Ventura Martina , Di Napoli Anteo , Mateo-Urdiales Alberto , Pezzotti Patrizio , Fabiani Massimo
Published: April 2023

Objectives

This study aimed to evaluate the differences in incidence, non-intensive care unit (non-ICU) and intensive care unit (ICU) hospital admissions, and COVID-19-related mortality between the “inner areas” of Italy and its metropolitan areas.

Study design

Retrospective population-based study conducted from the beginning of the pandemic in Italy (20 February 2020) to 31 March 2022.

Methods

The municipalities of Italy were classified into metropolitan areas, peri-urban/intermediate areas and “inner areas” (peripheral/ultra-peripheral). The exposure variable was residence in an “inner area” of Italy. Incidence of diagnosis of SARS-CoV-2 infection, non-ICU and ICU hospital admissions and death within 30 days from diagnosis were the outcomes of the study. COVID-19 vaccination access was also evaluated. Crude and age-standardized rates were calculated for all the study outcomes. The association between the type of area of residence and each outcome under study was evaluated by calculating the ratios between the standardized rates. All the analyses were stratified by period of observation (original Wuhan strain, Alpha variant, Delta variant, Omicron variant).

Results

Incidence and non-ICUs admissions rates were lower in “inner areas.” ICU admission and mortality rates were much lower in “inner areas” in the early phases of the pandemic, but this protection progressively diminished, with a slight excess risk observed in the “inner areas” during the Omicron period. The greater vaccination coverage in metropolitan areas may explain this trend.

Conclusion

Prioritizing healthcare planning through the strengthening of the primary prevention policies in the peripheral areas of Italy is fundamental to guarantee health equity policies.
Antioxidant enzyme and DNA base repair genetic risk scores’ associations with systemic oxidative stress biomarker in pooled cross-sectional studies
Authors: Mao Ziling , Gray Abigail L. H. , Thyagarajan Bharat , Bostick Roberd M.
Published: April 2023

Background: Oxidative stress is hypothesized to contribute to the pathogenesis of several chronic diseases. Numerous dietary and lifestyle factors are associated with oxidative stress; however, little is known about associations of genetic factors, individually or jointly with dietary and lifestyle factors, with oxidative stress in humans.

Methods: We genotyped 22 haplotype-tagging single nucleotide polymorphisms (SNPs) in 3 antioxidant enzyme (AE) genes and 79 SNPs in 14 DNA base excision repair (BER) genes to develop oxidative stress-specific AE and BER genetic risk scores (GRS) in two pooled cross-sectional studies (n = 245) of 30–74-year-old, White, cancer- and inflammatory bowel disease-free adults. Of the genotypes, based on their associations with a systemic oxidative stress biomarker, plasma F2-isoprostanes (FiP) concentrations, we selected 4 GSTP1 SNPs for an AE GRS, and 12 SNPs of 5 genes (XRCC1, TDG, PNKP, MUTYH, and FEN1) for a BER GRS. We also calculated a previously-reported, validated, questionnaire-based, oxidative stress biomarker-weighted oxidative balance score (OBS) comprising 17 anti- and pro-oxidant dietary and lifestyle exposures, with higher scores representing a higher predominance of antioxidant exposures. We used general linear regression to assess adjusted mean FiP concentrations across GRS and OBS tertiles, separately and jointly.

Results: The adjusted mean FiP concentrations among those in the highest relative to the lowest oxidative stress-specific AE and BER GRS tertiles were, proportionately, 11.8% (p = 0.12) and 21.2% (p = 0.002) higher, respectively. In the joint AE/BER GRS analysis, the highest estimated mean FiP concentration was among those with jointly high AE/BER GRS. Mean FiP concentrations across OBS tertiles were similar across AE and BER GRS strata.

Conclusion: Our pilot study findings suggest that DNA BER, and possibly AE, genotypes collectively may be associated with systemic oxidative stress in humans, and support further research in larger, general populations.
The future potential of virtual reality countermeasures for maintaining behavioural health during long duration space exploration
Authors: Thomas Laura J.
Published: April 2023

Long duration space exploration is no longer a fantasy, with Elon Musk claiming to launch astronauts to Mars as early as 2029. The substantial increase in spaceflight duration required for a Mars mission has resulted in a stronger focus on behavioural health outcomes at NASA, with increased interest in using virtual reality countermeasures to both monitor and promote psychological wellbeing. From the perspective of a practitioner psychologist, this paper first considers the utility of virtual reality assessment of emerging behavioural health concerns for remote monitoring purposes. Key opportunities include using virtual reality for functional cognitive testing and leveraging the predictive abilities of multimodal data for personalised insights into symptomology. Suggestions are given as to how astronauts can self-monitor usage of virtual leisure activities that facilitate positive emotional experiences. Secondly, the potential to develop virtual reality countermeasures to deliver semi-structured therapeutic interventions such as collaborative cognitive-behavioural formulation in the absence of real-time communication is discussed. Finally, considerations for the responsible implementation of psychological monitoring tools are reviewed within a context of fostering psychological safety and reducing stigma.
Maize metabolomics in relation to cropping system and growing year
Authors: Mattoo Autar K. , Cavigelli Michel A. , Mišić Danijela M. , Gašić Uroš , Maksimović Vuk M. , Kramer Matthew , Kaur Bhavneet , Matekalo Dragana , Nestorović Živković Jasmina , Roberts Daniel P.
Published: April 2023

Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
A comprehensive assessment of self-reported symptoms among patients harboring an unruptured intracranial aneurysm
Authors: Hackett Ashia M. , Koester Stefan W. , Rhodenhiser Emmajane G. , Scherschinski Lea , Rulney Jarrod D. , Naik Anant , Nico Elsa , Eberle Adam T. , Hartke Joelle N. , Fox Brandon M. , Winkler Ethan A. , Catapano Joshua S. , Lawton Michael T.
Published: April 2023

Background

Approximately 3.2%–6% of the general population harbor an unruptured intracranial aneurysm (UIA). Ruptured aneurysms represent a significant healthcare burden, and preventing rupture relies on early detection and treatment. Most patients with UIAs are asymptomatic, and many of the symptoms associated with UIAs are nonspecific, which makes diagnosis challenging. This study explored symptoms associated with UIAs, the rate of resolution of such symptoms after microsurgical treatment, and the likely pathophysiology.

Methods

A retrospective review of patients with UIAs who underwent microsurgical treatment from January 1, 2014, to December 31, 2020, at a single quaternary center were identified. Analyses included the prevalence of nonspecific symptoms upon clinical presentation and postoperative follow-up; comparisons of symptomatology by aneurysmal location; and comparisons of patient demographics, aneurysmal characteristics, and poor neurologic outcome at postoperative follow-up stratified by symptomatic versus asymptomatic presentation.

Results

The analysis included 454 patients; 350 (77%) were symptomatic. The most common presenting symptom among all 454 patients was headache (n = 211 [46%]), followed by vertigo (n = 94 [21%]), cognitive disturbance (n = 68[15%]), and visual disturbance (n = 64 [14%]). Among 328 patients assessed for postoperative symptoms, 258 (79%) experienced symptom resolution or improvement.

Conclusion

This cohort demonstrates that the clinical presentation of patients with UIAs can be associated with vague and nonspecific symptoms. Early detection is crucial to prevent aneurysmal subarachnoid hemorrhage. It is imperative that physicians not rule out aneurysms in the setting of nonspecific neurologic symptoms.
The Utility of Minimally Invasive Surgery in the Emergency Management of Femoral Hernias: A Systematic Review
Authors: Shuttleworth Paul , Sabri Shariq , Mihailescu Andrei
Published: April 2023

Background: Femoral hernias are a relatively rare type of hernia but have a high complication rate, with a high proportion either presenting as an emergency or requiring emergency management. Minimal access surgery has been shown to be safe, with good results, in an elective setting, but there is little published evidence of its utility in an emergency.

Methods: A systematic review was conducted searching PubMed, OVID, Embase, and Cochrane reviews for ((Femoral hernia) AND (laparoscop* OR minimal access OR robotic)) AND (strangulat* OR obstruct* OR incarcerat*).

Results: 286 manuscripts were identified of which 33 were relevant. 24 were individual case reports, 3 case series, 4 cohort studies or case control series, and 2 high level reviews of National registers.

Conclusion: Minimal access surgery can avoid an unnecessary laparotomy for the assessment of hernial contents, especially via a TAPP approach. Minimal access repair of femoral hernias as an emergency is feasible and can be done safely with results similar to open surgery but good quality evidence is lacking.
Does Digital Technology Application Promote Carbon Emission Efficiency in Dairy Farms? Evidence from China
Authors: Liu Chenyang , Wang Xinyao , Bai Ziming , Wang Hongye , Li Cuixia
Published: April 2023

The implementation of digital technology has become paramount to facilitating green and low-carbon development in dairy farms amidst the advent of digital agriculture and low-carbon agriculture. This study examined the impact of digital technology implementation on the carbon emission efficiency of Chinese dairy farms via an assessment of micro-survey data, incorporating an Undesirable Outputs-SBM model, a Tobit model, the propensity score matching technique, a quantile regression model, and an instrumental variable approach. This study examined the potential moderating influence of environmental regulations on digital technology applications and the carbon emission efficiency of dairy farms. The findings of the research indicate that the implementation of digital technology had a considerable beneficial consequence on the carbon emission proficiency of dairy farms. The statistical significance level of the mean treatment effect was 0.1161, with the most profound influence of precision feeding digital technology on the carbon emission efficiency in dairy farms. The application of digital technology has a more pronounced effect on dairy farms with lower levels of carbon emission efficiency compared to those with medium and high levels of carbon emission efficiency. The application of digital technology toward the carbon emission efficiency of dairy farms is positively moderated by environmental regulations. Finally, this paper puts forward some specific policy recommendations to achieve the strategic goal of low carbon and efficient development in dairy farms through the application of digital technology, which enriches the existing research on carbon emission reduction in dairy farms from theoretical and practical aspects.
Analysis of the Spatio-Temporal Evolution, Influencing Factors, and Spillover Effects of the Urban–Rural Income Gap in Chongqing Municipality, China
Authors: Yang Shiqin , Yang Zisheng , Yang Renyi , Cai Xueli
Published: April 2023

In addition to being necessary for the stability, coordination, and sustainable growth of the national economy, narrowing the urban–rural income gap is also an “Important national matter” for the long-term security of the nation. “Big mountain areas, big cities, big reservoir areas, big rural areas, and ethnic areas” are all present in the municipality of Chongqing. All of the poverty-stricken counties have been lifted out of poverty, despite the promotion of targeted poverty alleviation and other policies, significant urban–rural income gaps remain. In view of the current research, there has been no in-depth discussion on the correlation between urban and rural income gap and poverty levels in various regions, and there has been no in-depth discussion on the spatial correlation and spillover effects of various influencing factors. This paper employs panel data from 2010 to 2021 for 37 counties in Chongqing; based on an analysis of the characteristics of the urban–rural income gap’s spatial pattern and spatio-temporal evolution, it classifies each county (city and district) as either a non-poverty or poverty-stricken county and uses spatial econometric models to conduct an in-depth study of the influencing factors and spillover effects of the urban–rural income gap in Chongqing. The outcomes of our analysis of the influencing factors reveal that the level of fixed assets investment, the per capita spending of public funds, the proportion of rural employees, the proportion of grain sowing, the amount of agricultural fertilizer applied per unit area, the proportion of real estate development investment, and population density variables are important causes of the URIG in Chongqing. The spillover effects of these factors vary between poverty-stricken and non-poverty-stricken counties. This paper aims to provide reference to policymakers to design measures to narrow the urban–rural income gap and advance the urban–rural coordinated development strategy on the basis of a thorough examination of the spatial and temporal evolution, influencing variables, and spillover effects of the urban–rural income gap in Chongqing.
From Waste to Resources: Sewage Sludges from the Citrus Processing Industry to Improve Soil Fertility and Performance of Lettuce (Lactuca sativa L.)
Authors: Lucia Caterina , Pampinella Daniela , Palazzolo Eristanna , Badalucco Luigi , Laudicina Vito Armando
Published: April 2023

The citrus industry produces a large number of sludges as a consequence of citrus wastewater treatment. The correct disposal of citrus sewage sludges (CSSs) has been attempted using anaerobic digestion, aerobic digestion, and lime stabilization. However, since CSSs hold nitrogen, phosphorus, and other macronutrients required by crops, in line with the circular economy principles, they could be utilized for agricultural purposes, such as organic fertilizer. The aim of this study was to assess the effect of CSSs supplied at different doses on soil fertility and lettuce performance. To this end, a pot experiment was established. The soil was amended with CSSs at three different concentrations (2.5, 5, 10 t ha−1). After 46 days of lettuce growth, the experiment was stopped, and soils and plants were analyzed. Soil amended with CSSs showed an increase in total organic C ranging from 7% to 11%. Additionally, available P increased but only at the highest CSS dose. The addition of CSSs affected the biochemical properties of soil, but a univocal trend related to the number of CSSs applied was not found. Microbial biomass C increased only with the highest dose of CSS applied, while the metabolic quotient (qCO2) decreased. Such a positive effect on soil fertility and soil microorganisms, in turn, lead to an increase in lettuce biomass. Moreover, results indicated that following CSS addition, lettuce crops adsorbed more N in leaves than in roots, whereas P, Ca, Mg, K, and Na showed an opposite pattern and increased more consistently in roots. In conclusion, amendment with CSSs enhances soil fertility by increasing, regardless of CSS dose, total organic C, and, at the highest dose, P availability and microbial biomass C. Such improvement in soil fertility, in turn, increases lettuce biomass production without affecting its quality, i.e., alteration of the (K + Na)/(Ca + Mg) ratio.
Fate of Copper in Saline–Alkali Soil with Long-Term Application of Biogas Residue
Authors: Liu Binhao , Wang Shengxiao , Dong Pengcheng , Zhang Xinzhe , Zhang Long , Chen Chen , Xu Xihui , Xia Yan , Shen Zhenguo , Shi Liang , Chen Yahua
Published: April 2023

The retention of copper (Cu) in saline–alkali soil (SAS) during long-term application of biogas residue (BR) with a high concentration of Cu raises concerns. In this work, the fate of Cu was detected using adsorption isotherms, scanning electron microscope—energy dispersive spectrometer, Fourier transform infrared spectrometer, X-ray diffraction, isothermal titration calorimetry, X-ray photoelectron spectroscopy, and microzone X-ray fluorescence spectrometer. The results showed that the main groups for Cu adsorption by SAS and BR were carboxyl, hydroxyl, amide and amine. The adsorption of Cu by the carboxyl group was entropy–enthalpy co-driven (|ΔH| < |TΔS|, ΔH < 0). The adsorption of Cu by the amine group was entropy-driven (|ΔH| > |TΔS|, ΔH > 0). The adsorption of Cu on the SAS and BR was achieved by organic matter rather than minerals. The degradation of BR in the SAS increases the content of Cu adsorption groups such as carboxyl and amine groups, and Cu was adsorbed on the surface or inside SAS through organic groups. This study provides further theoretical support for the application of BR in SAS.
Non-Destructive Appraisal of Macro- and Micronutrients in Persimmon Leaves Using Vis/NIR Hyperspectral Imaging
Authors: Acosta Maylin , Rodríguez-Carretero Isabel , Blasco José , de Paz José Miguel , Quiñones Ana
Published: April 2023

Visible and near-infrared (Vis/NIR) hyperspectral imaging (HSI) was used for rapid and non-destructive determination of macro- and micronutrient contents in persimmon leaves. Hyperspectral images of 687 leaves were acquired in the 500–980 nm range over 6 months, covering a complete vegetative cycle. The average reflectance spectrum of each leaf was extracted, and foliar ionomic analysis was used as a reference method to determine the actual concentration of the nutrients in the leaves. Analyses were performed via emission spectrometry (ICP-OES) for macro- and micronutrients after microwave digestion and using the Kjeldahl method to quantify nitrogen. Partial least square regression (PLS-R) was used to predict the nutrient concentration based on spectral data from the leaf using actual values of each element as predictor variables. Several methods were used to pre-process the spectra, including Savitzky–Golay (SG) smoothing, standard normal variate (SNV) and first (1D) and second derivatives (2D). Seventy-five percent of the samples were used to calibrate and validate the model by cross-validation, whereas the remaining twenty-five % were used as an independent test set. The best performance of the models for the test set achieved an R2 = 0.80 for nitrogen. Results were also satisfactory for phosphorous, calcium, magnesium and boron, with determination coefficient R2 values of 0.63, 0.66, 0.58 and 0.69, respectively. For the other nutrients, lower prediction rates were attained (R2 = 0.48 for potassium, R2 = 0.38 for iron, R2 = 0.24 for copper, R2 = 0.23 for zinc and R2 = 0.22 for manganese). The variable importance in projection (VIP) was used to extract the most influential bands for the best-predicted nutrients, which were N, K and B.
Evaluation of Inoculation Methods for Determination of Winter Wheat Resistance to Fusarium Head Blight
Authors: Šarčević Hrvoje , Bukan Miroslav , Lovrić Ana , Maričević Marko
Published: April 2023

One of the most severe winter wheat (Triticum aestivum L.) diseases is Fusarium head blight (FHB). It is believed that selection for resistance to FHB is better in high disease pressure environments, for which various methods of artificial inoculation are used. The standard spray method of artificial inoculation is believed to be technically demanding and labour intensive. Therefore, scattering Fusarium-infected maize stalks onto trial plots after wheat emergence is suggested as a suitable alternative. The aim of this study was to compare the mean values and heritability of the visual rating index (VRI) and the percentage of Fusarium-damaged kernels (FDK) between the two abovementioned methods of artificial inoculation and natural infection, and to determine the phenotypic correlations between the three methods for the studied traits. The achieved levels of VRI and FDK were comparable for the two methods of artificial inoculation and considerably lower under natural conditions. Heritability for VRI ranged over four years from 0.68 to 0.91 for the spray method, from 0.73 to 0.95 for the infected maize stalks, and from 0.26 to 0.65 for natural infection, whereas for FDK it ranged from 0.56 to 0.85, 0.38 to 0.83, and 0.11 to 0.44 for the three inoculation methods, respectively. The strong positive correlation between the two applied methods of artificial inoculation for studied traits suggests that scattering infected maize stalks could serve as a reliable supplement for the technically and labor-intensive spray method of artificial inoculation.
Impact of Sandy Soil Amendment with Dairy Slurry Treated through pH Adjustment on Nutrient and Coliform Leaching
Authors: Rodrigues Joana , Fragoso Rita , Brito Luísa , Fangueiro David
Published: April 2023

Livestock farming produces high volumes of animal slurry that can be sanitized using low-cost treatments by pH adjustment, reducing pathogen contamination, and promoting slurry valorization as a safe fertilizer. This work aims to evaluate the impact of sandy soil amendment with dairy slurry treated by pH adjustment on the potential release of nutrients and coliforms into groundwater. A laboratory soil column leaching experiment was conducted and the surface application of six treatments was tested: raw dairy slurry, dairy slurry acidified with H2SO4, dairy slurry alkalinized with KOH, alkalinized/neutralized dairy slurry, mineral fertilizer, and a control. The fertilizer application rate was 0.08 g N kg−1 dry soil. Leachates were analyzed for pH, electrical conductivity, N-NH4+ and N-NO3−, macro- and micronutrients, and fecal coliforms. Results showed that amendment with dairy slurry led to significantly lower nitrate leaching potential than mineral fertilizer (maximum 16.1 vs. 50.4%). The use of hygienized slurry by alkalinization decreased the potential leaching of coliforms relative to raw slurry. However, incomplete sanitization by acidification strongly promoted coliform leaching and the risk of groundwater contamination. It can be concluded that the use of alkalinized dairy slurry is a safer solution than raw slurry with regard to the risk of groundwater pollution.
Modeling the Kinematic Response of Rice under Near-Ground Wind Fields Using the Finite Element Method
Authors: Hu Xiaodan , Li Huifen , Wu Han , Long Bo , Liu Zhijie , Wei Xu , Li Jiyu
Published: April 2023

Plant protection drones are commonly encountered in agricultural fields. Their downwash airflow can agitate flexible crops (e.g., rice and wheat) or even cause wind-induced losses. To predict the wind-induced responses of rice under wind fields, herein, a wind-induced rice response model (RWRM) was proposed using the finite element method. With the RWRM, the rice displacement and critical wind speed (CWS) were calculated at different wind speeds, considering the morphological and mechanical properties of rice, and the accuracy was experimentally verified and compared to that of an existing model. The results indicated that the mean paired difference and mean error in rice displacement amplitude prediction under 2~5 m/s wind speeds were 13.48 mm and 42.46 mm, respectively, and the predicted and measured values were highly correlated at the 1% significance level. Moreover, the CWS values for four rice species could be calculated with the model with an average of 3.57 m/s, and the difference between the simulated and theoretical values was 0.368. The strength of the wind-induced rice responses was primarily correlated with the mechanical properties, and to a lesser extent the morphology. The rice yield has a negative correlation with rice responses. Within a certain range, a bigger displacement and lower CWS could result in a higher rice yield. The RWRM achieved favorable modeling accuracy for the wind-induced responses of rice and could provide a simulation reference for balancing the wind-induced loss and rice yield.
Effects of High Concentrate-Induced Subacute Ruminal Acidosis Severity on Claw Health in First-Lactation Holstein Cows
Authors: Kofler Johann , Hoefler Michael , Hartinger Thomas , Castillo-Lopez Ezequias , Huber Johann , Tichy Alexander , Reisinger Nicole , Zebeli Qendrim
Published: April 2023

This study aimed to evaluate the effects of diet-induced subacute rumen acidosis (SARA) severity during transition and the early lactation period on claw health in 24 first-lactation Holstein heifers. All heifers were fed a 30% concentrate (in dry matter) close-up ration three weeks before calving, then switched to a high-concentrate ration (60% dry matter), which was fed until the 70th day in milk (DIM) to induce SARA. Thereafter, all cows were fed the same post-SARA ration with around 36% concentrate in dry matter. Hoof trimming was performed before calving (visit 1), at 70 (visit 2) and at 160 DIM (visit 3). All claw lesions were recorded, and a Cow Claw Score (CCS) was calculated for each cow. Locomotion scores (LCS 1–5) were assessed at two-week intervals. Intraruminal sensors for continuous pH measurements were used to determine SARA (pH below 5.8 for more than 330 min in 24 h). The cluster analysis grouped the cows retrospectively into light (≤11%; n = 9), moderate (>11–<30%; n = 7), and severe (>30%; n = 8) SARA groups, based on the percentage of days individual cows experienced SARA. Statistically significant differences were found between SARA groups light and severe in terms of lameness incidence (p = 0.023), but not for LCS and claw lesion prevalence. Further, the analysis of maximum likelihood estimates revealed that for each day experiencing SARA, the likelihood of becoming lame increased by 2.52% (p = 0.0257). A significant increase in white line lesion prevalence was observed between visits 2 and 3 in the severe SARA group. The mean CCS in severe SARA group cows were higher at each visit compared to cows in the other two groups, but without statistical significance. Overall, this is the first study indicating that first-lactation cows fed a similar high-concentrate diet but with a higher severity of SARA tended to have poorer claw health, albeit with only partial statistical evidence.
Frequency and Characterisation of Anomalies and Fractures of the Caudal Spine in Sheep with Undocked Tails
Authors: Hümmelchen Hannah , Wagner Henrik , Brügemann Kerstin , Wenisch Sabine , König Sven , Wehrend Axel
Published: April 2023

As tails are often docked within the first days of life, studies investigating tail malformations and injuries in sheep do not exist thus far. To address this gap in the literature, this research aimed to analyse the occurrence of vertebral anomalies and fractures in the tail within an undocked Merinoland sheep population. At 14 weeks of age, the caudal spines of 216 undocked Merinoland lambs was radiographically examined, and tail length and circumference were measured. Anomalies were documented and statistical correlation and model calculations were performed. The occurrence of block vertebrae was observed in 12.96% and wedged vertebrae in 8.33% of the sample. Of the animals, 59 (27.31%) exhibited at least one vertebral fracture, which were observed in the middle and caudal third of the tail. A significant correlation was found between the occurrence of fractures and tail length (r = 0.168) and number of vertebrae (r = 0.155). Conversely, the presence of block and wedged vertebrae was not significantly correlated with tail length, circumference, or number of vertebrae. Only the sex showed significant differences in the probability of axis deviation. These results emphasize the importance of breeding for short tails to avoid fractures.
Mandan, Hidatsa, and Arikara Nation Perspectives on Rez Dogs on the Fort Berthold Reservation in North Dakota, U.S.A.
Authors: Cardona Alexandra , Hawes Sloane M. , Cull Jeannine , Connolly Katherine , O’Reilly Kaleigh M. , Moss Liana R. , Bexell Sarah M. , Yellow Bird Michael , Morris Kevin N.
Published: April 2023

The research on the relationships between free-roaming dogs, also referred to as reservation dogs or rez dogs, and Indigenous communities is extremely limited. This study aimed to document the cultural significance of rez dogs, challenges related to rez dogs, and community-specific solutions for rez dog issues affecting community health and safety from members of the Mandan, Hidatsa, and Arikara (MHA) Nation, also referred to as the Three Affiliated Tribes (TAT), who live on the Fort Berthold reservation in North Dakota, U.S.A. One hour semi-structured interviews with 14 community members of the MHA Nation were conducted in 2016. The interviews were analyzed via systematic and inductive coding using Gadamer’s hermeneutical phenomenology. The primary intervention areas described by the participants included: culturally relevant information sharing, improved animal control policies and practices, and improved access to veterinary care and other animal services.
Life History Parameters and Fishing Aspects of the Alien Nimble Spray Crab Percnon gibbesi in a Native Area of the Central-East Atlantic
Authors: Guerra-Marrero Airam , Bonino-Pérez Antonio , Espino-Ruano Ana , Couce-Montero Lorena , Jiménez-Alvarado David , Castro José
Published: April 2023

Percnon gibbesi is a native crab species characteristic of intertidal and subtidal zones of the Atlantic coast of the European Macaronesian archipelagos (Azores, Madeira, and Canary Islands), and probably also in the neighbouring rocky coasts of northwest Africa. P. gibbesi is considered an invasive alien species in almost all of the Mediterranean, with expanding populations from Spain to Turkey, including Libya. However, its biology and ecology are highly unknown, despite all its range of distribution. In the intertidal zones of Gran Canaria Island, this crab, in the intertidal zones of Gran Canaria Island, shows a carapace length range between 4.1 and 22.7 mm (4.1–22.7 in males and 5.7–22.3 in females), where females showed higher weights and lengths than males on average. However, males predominated in all samples, with a sex ratio of 1:0.57. The L∞ for this crab was estimated to be 27 ± 3 mm (23 ± 4 mm for females and 25 ± 4 mm for males). The growth coefficient (K) was 0.24 year−1, the total mortality (Z) was Z = 1.71 year−1, and the natural mortality (M) was 0.47 year−1. Although females grow faster than males, males are more abundant in the larger length classes. Although the presence of ovigerous females indicated that reproduction takes place twice a year, from March to April and from August to September, the number of cohorts detected by the modal progression analysis showed that reproduction takes place all year.