bluuebunny/crossref_metadata_2025 huggingface dataset:
Endoglin aggravates peritoneal fibrosis by regulating the activation of TGF-β/ALK/Smads signaling
Authors: Huang Qian , Xiao Rui , Lu Jing , Zhang Yao , Xu Liang , Gao Jie , Sun Jing , Wang Haiping
Published: September 2022

Background: Peritoneal fibrosis (PF) is an intractable complication in patients on long-term peritoneal dialysis (PD). Transforming growth factor-β (TGF-β) is a key pro-fibrogenic factor involved in PD-associated PF, and endoglin, as a coreceptor for TGF-β, plays a role in balancing the TGF-β signaling pathway. Here, we investigated whether endoglin could be a potential therapeutic target for PF.

Methods:In vivo, we established PF model in SD rats by daily intraperitoneal injection of peritoneal dialysis fluids (PDF) containing 4.25% glucose for 6 weeks and downregulated endoglin expression by tail vein injection of AAV9-ENG on day 14 to assess the effect of endoglin on peritoneal morphology and markers related to fibrosis, angiogenesis, and epithelial-mesenchymal transition (EMT). In vitro, we treated human peritoneal mesothelial cells (HPMCs) transfected with ENG siRNA in high glucose medium to explore the potential mechanism of endoglin in PF.

Results: Compared to control group, continuous exposure to biologically incompatible PDF induced exacerbated PF, accompanied by a significant increase in endoglin expression. Conversely, knockdown of endoglin ameliorated peritoneal injury characterized by increased peritoneal thickening and collagen deposition, angiogenesis, as well as EMT. Consistently, HPMCs cultured in high glucose medium underwent the EMT process and exhibited over-expression of fibronectin, collagen type I, vascular endothelial growth factor (VEGF), whereas these aforementioned alterations were alleviated after ENG siRNA transfection. In addition, we also found that ENG siRNA inhibited TGF-β-induced phosphorylation of Smad2/3 and Smad1/5/9 in HPMCs treated with high glucose (HG).

Conclusion: Our findings confirmed for the first time that endoglin exacerbated PF by regulating the activation of TGF-β/ALK/Smads signaling, which will provide a novel potential therapeutic target in PF.
New developments in econophysics: Option pricing formulas
Authors: Alghalith Moawia
Published: September 2022

We synthesize and discuss some new developments in econophysics. In doing so, we focus on option pricing. We relax the assumptions of constant volatility and interest rate. In doing so, we rely on the square root of the Brownian motion. We also provide simple, closed-form pricing formulas for the American and Bermudan options.
Quantum dot scanning tunneling microscopy for Majorana bound states in continuum
Authors: Zhang Hai-Rui , Sun Yong-Ping
Published: September 2022

We propose a device composed of a quantum dot (QD) connected to a normal metal lead to detect Majorana bound states (MBSs), which are formed at the ends of a topological superconductor nanowire (TSNW) and coupled to the lead with spin-dependent hybridization strengths. The information of the MBSs leaked into the lead is inferred from the spectral function of the QD serving as the tip of a scanning tunneling microscope (STM). It is found that lead–MBSs interaction induces a bound state characterized by an infinitely high peak in the dot’s zero-energy spectral function. The overlap between the two modes of the MBSs turns this bound state into a resonant one, and thus the zero-energy peak is split into three with the height of the central one equaling that in the absence of lead–MBSs coupling. We also find that the MBSs have lower impacts on the additional peak in the dot’s spectral function induced by intradot Coulomb interaction.
NRF2: A crucial regulator for mitochondrial metabolic shift and prostate cancer progression
Authors: Buttari Brigitta , Arese Marzia , Oberley-Deegan Rebecca E. , Saso Luciano , Chatterjee Arpita
Published: September 2022

Metabolic alterations are a common survival mechanism for prostate cancer progression and therapy resistance. Oxidative stress in the cellular and tumor microenvironment dictates metabolic switching in the cancer cells to adopt, prosper and escape therapeutic stress. Therefore, regulation of oxidative stress in tumor cells and in the tumor-microenvironment may enhance the action of conventional anticancer therapies. NRF2 is the master regulator for oxidative stress management. However, the overall oxidative stress varies with PCa clinical stage, metabolic state and therapy used for the cancer. In agreement, the blanket use of NRF2 inducers or inhibitors along with anticancer therapies cause adverse effects in some preclinical cancer models. In this review, we have summarized the levels of oxidative stress, metabolic preferences and NRF2 activity in the different stages of prostate cancer. We also propose condition specific ways to use NRF2 inducers or inhibitors along with conventional prostate cancer therapies. The significance of this review is not only to provide a detailed understanding of the mechanism of action of NRF2 to regulate oxidative stress-mediated metabolic switching by prostate cancer cells to escape the radiation, chemo, or hormonal therapies, and to grow aggressively, but also to provide a potential therapeutic method to control aggressive prostate cancer growth by stage specific proper use of NRF2 regulators.
SLC38A2 provides proline and alanine to regulate postnatal bone mass accrual in mice
Authors: Shen Leyao , Yu Yilin , Karner Courtney M.
Published: September 2022

Amino acids have recently emerged as important regulators of osteoblast differentiation and bone formation. Osteoblasts require a continuous supply of amino acids to sustain biomass production to fuel cell proliferation, osteoblast differentiation and bone matrix production. We recently identified proline as an essential amino acid for bone development by fulfilling unique synthetic demands that are associated with osteoblast differentiation. Osteoblasts rely on the amino acid transporter SLC38A2 to provide proline to fuel endochondral ossification. Despite this, very little is known about the function or substrates of SLC38A2 during bone homeostasis. Here we demonstrate that the neutral amino acid transporter SLC38A2 is expressed in osteoblast lineage cells and provides proline and alanine to osteoblast lineage cells. Genetic ablation of SLC38A2 using Prrx1Cre results in decreased bone mass in both male and female mice due to a reduction in osteoblast numbers and bone forming activity. Decreased osteoblast numbers are attributed to impaired proliferation and osteogenic differentiation of skeletal stem and progenitor cells. Collectively, these data highlight the necessity of SLC38A2-mediated proline and alanine uptake during postnatal bone formation and bone homeostasis.
HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley
Authors: Feng Xiuqin , Rahman Md. Mostafijur , Hu Qian , Wang Bang , Karim Hassan , Guzmán Carlos , Harwood Wendy , Xu Qiang , Zhang Yazhou , Tang Huaping , Jiang Yunfeng , Qi Pengfei , Deng Mei , Ma Jian , Lan Jingyu , Wang Jirui , Chen Guoyue , Lan Xiujin , Wei Yuming , Zheng Youliang , Jiang Qiantao
Published: September 2022

Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3′ splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5′ of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism.
Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
Authors: Si Cheng , Yang Shipeng , Lou Xiangyun , Zhang Guangnan , Zhong Qiwen
Published: September 2022

In the present study, we determined the morphological and physiological indicators of Pepino to elucidate its lateral branching responses to different light qualities using a full-spectrum lamp (F) as the control and eight different light ratios using blue light (B) and red light (R). In addition, correlation analysis revealed that the gene expression patterns correlated with lateral branching under various light treatments. Compared with the F treatment, the R treatment increased the plant height and inhibited the elongation of lateral branches, in contrast with the B treatment. The number of lateral branches did not change significantly under different light quality treatments. Moreover, correlation analysis showed that the ratio of blue light was significantly positively correlated with the length of lateral branches and significantly negatively correlated with plant height, aboveground dry weight, and other indicators. We conducted transcriptome sequencing of the sites of lateral branching at three periods under different light quality treatments. The gene related to photodynamic response, cryptochrome (CRY), was the most highly expressed under B treatment, negatively regulated lateral branch length, and positively correlated with plant height. Branched 1, a lateral branch regulation gene, was upregulated under R treatment and inhibited branching. Overall, the red light facilitated internode elongation, leaf area expansion, plant dry weight increase, and inhibition of lateral branching. Soluble sugar content increased, and the lateral branches elongated under blue light. Different light qualities regulated lateral branching by mediating different pathways involving strigolactones and CRY. Our findings laid a foundation for further clarifying the response mechanism of Pepino seedlings to light and provided a theoretical reference for elucidating the regulation of different light qualities on the lateral branching of Pepino.
Long-term fertilization has different impacts on bacterial communities and phosphorus forms in sugarcane rhizosphere and bulk soils under low-P stress
Authors: Wu Qihua , Chen Diwen , Zhou Wenling , Zhang Xingxing , Ao Junhua
Published: September 2022

The application of phosphorus (P) fertilizer effectively improves soil P availability, but it also affects soil microbial communities. However, the responses of soil bacterial communities and P forms to long-term P fertilization, and the relationships of bacterial communities with soil P forms remain unclear in P-deficient field. In this study, the impacts of different P fertilization treatments (chemical nitrogen and potassium (NK); chemical N, P and K (NPK); and NPK plus straw (NPKS)) on the bacterial communities and P forms in sugarcane rhizosphere (RS) and bulk soils (BS) were evaluated. Compared with the NK, the NPK and NPKS treatments significantly (P<0.05) increased the yield and quality characters of sugarcane, especially under NPKS. Additionally, P fertilization significantly increased the available P (AP), soluble inorganic P (Pi) and retained Pi in both the RS and BS, but they significantly increased the Chao1 and Shannon index only in the BS; and almost all these indices were significantly higher in the RS than in the BS. The bacterial community compositions were also significantly altered by P fertilization, with major changes in the RS and minor changes in the BS. The bacterial genera that were enriched in the sugarcane rhizosphere mainly includedBradyrhizobium,Rhodanobacter,Pseudolabrys,Conexibacter, andBurkholderia-Caballeronia-Paraburkholderia, some of which potentially promote the plant growth. Compared to NK, functional groups involved in the cycling of carbon, N, and sulfur significantly increased or decreased with fertilizer P application. Moreover, the relative abundances of many bacterial species were significantly correlated with the soil P forms. In conclusion, long-term P fertilization altered bacterial structure and functions in P-deficient sugarcane soil, which could help the soil P cycling and suppling. The results provide useful information to stimulate the power of the microbes by fertilization measures to improve soil nutrients and crop production.
Functional analysis of the BEige and Chediak-Higashi domain gene MpSPIRRIG in Marchantia polymorpha
Authors: Koebke Eva , Stephan Lisa , Stetter Markus G. , Hülskamp Martin
Published: September 2022

BEige and Chediak–Higashi domain containing proteins (BDCPs) have been described to function in membrane-dependent processes in eukaryotes. This role was also observed for the BDCP SPIRRIG (SPI) in the model plant Arabidopsis thaliana in the context of cell morphogenesis. Additionally, AtSPI was found to control salt stress resistance by mediating mRNA stability and salt stress-dependent processing body formation. In this work, we utilize an evolutionarily comparative approach to unravel conserved, basal BDCP functions in the liverwort Marchantia polymorpha. Our phenotypic and physiological analyses show that MpSPI is involved in cell morphogenesis and salt resistance regulation, indicating that both functions are evolutionarily conserved between the two species. Co-localization was found with endosomal and P-body markers, suggesting links to membrane-dependent processes and mRNA metabolism. Finally, we present transcriptomics data showing that AtSPI and MpSPI regulate orthologous genes in A. thaliana and M. polymorpha.
United States tea: A synopsis of ongoing tea research and solutions to United States tea production issues
Authors: D’Auria John C. , Cohen Stephen P. , Leung Jason , Glockzin Kayla , Glockzin Kyle Mark , Gervay-Hague Jacquelyn , Zhang Dapeng , Meinhardt Lyndel W.
Published: September 2022

Tea is a steeped beverage made from the leaves of Camellia sinensis. Globally, this healthy, caffeine-containing drink is one of the most widely consumed beverages. At least 50 countries produce tea and most of the production information and tea research is derived from international sources. Here, we discuss information related to tea production, genetics, and chemistry as well as production issues that affect or are likely to affect emerging tea production and research in the United States. With this review, we relay current knowledge on tea production, threats to tea production, and solutions to production problems to inform this emerging market in the United States.
A metabolic, phylogenomic and environmental atlas of diatom plastid transporters from the model species Phaeodactylum
Authors: Liu Shun , Storti Mattia , Finazzi Giovanni , Bowler Chris , Dorrell Richard G.
Published: September 2022

Diatoms are an important group of algae, contributing nearly 40% of total marine photosynthetic activity. However, the specific molecular agents and transporters underpinning the metabolic efficiency of the diatom plastid remain to be revealed. We performed in silico analyses of 70 predicted plastid transporters identified by genome-wide searches of Phaeodactylum tricornutum. We considered similarity with Arabidopsis thaliana plastid transporters, transcriptional co-regulation with genes encoding core plastid metabolic pathways and with genes encoded in the mitochondrial genomes, inferred evolutionary histories using single-gene phylogeny, and environmental expression trends using Tara Oceans meta-transcriptomics and meta-genomes data. Our data reveal diatoms conserve some of the ion, nucleotide and sugar plastid transporters associated with plants, such as non-specific triose phosphate transporters implicated in the transport of phosphorylated sugars, NTP/NDP and cation exchange transporters. However, our data also highlight the presence of diatom-specific transporter functions, such as carbon and amino acid transporters implicated in intricate plastid-mitochondria crosstalk events. These confirm previous observations that substrate non-specific triose phosphate transporters (TPT) may exist as principal transporters of phosphorylated sugars into and out of the diatom plastid, alongside suggesting probable agents of NTP exchange. Carbon and amino acid transport may be related to intricate metabolic plastid-mitochondria crosstalk. We additionally provide evidence from environmental meta-transcriptomic/meta- genomic data that plastid transporters may underpin diatom sensitivity to ocean warming, and identify a diatom plastid transporter (J43171) whose expression may be positively correlated with temperature.
Recent developments in multi-omics and breeding strategies for abiotic stress tolerance in maize (Zea mays L.)
Authors: Farooqi Muhammad Qudrat Ullah , Nawaz Ghazala , Wani Shabir Hussain , Choudhary Jeet Ram , Rana Maneet , Sah Rameswar Prasad , Afzal Muhammad , Zahra Zahra , Ganie Showkat Ahmad , Razzaq Ali , Reyes Vincent Pamugas , Mahmoud Eman A. , Elansary Hosam O. , El-Abedin Tarek K. Zin , Siddique Kadambot H. M.
Published: September 2022

High-throughput sequencing technologies (HSTs) have revolutionized crop breeding. The advent of these technologies has enabled the identification of beneficial quantitative trait loci (QTL), genes, and alleles for crop improvement. Climate change have made a significant effect on the global maize yield. To date, the well-known omic approaches such as genomics, transcriptomics, proteomics, and metabolomics are being incorporated in maize breeding studies. These approaches have identified novel biological markers that are being utilized for maize improvement against various abiotic stresses. This review discusses the current information on the morpho-physiological and molecular mechanism of abiotic stress tolerance in maize. The utilization of omics approaches to improve abiotic stress tolerance in maize is highlighted. As compared to single approach, the integration of multi-omics offers a great potential in addressing the challenges of abiotic stresses of maize productivity.
Analysis of herbivore-responsive long noncoding ribonucleic acids reveals a subset of small peptide-coding transcripts in Nicotiana tabacum
Authors: Jin Jingjing , Meng Lijun , Chen Kai , Xu Yalong , Lu Peng , Li Zhaowu , Tao Jiemeng , Li Zefeng , Wang Chen , Yang Xiaonian , Yu Shizhou , Yang Zhixiao , Cao Linggai , Cao Peijian
Published: September 2022

Long non-coding RNAs (lncRNAs) regulate many biological processes in plants, including defense against pathogens and herbivores. Recently, many small ORFs embedded in lncRNAs have been identified to encode biologically functional peptides (small ORF-encoded peptides [SEPs]) in many species. However, it is unknown whether lncRNAs mediate defense against herbivore attack and whether there are novel functional SEPs for these lncRNAs. By sequencing Spodoptera litura-treated leaves at six time-points in Nicotiana tabacum, 22,436 lncRNAs were identified, of which 787 were differentially expressed. Using a comprehensive mass spectrometry (MS) pipeline, 302 novel SEPs derived from 115 tobacco lncRNAs were identified. Moreover, 61 SEPs showed differential expression after S. litura attack. Importantly, several of these peptides were characterized through 3D structure prediction, subcellular localization validation by laser confocal microscopy, and western blotting. Subsequent bioinformatic analysis revealed some specific chemical and physical properties of these novel SEPs, which probably represent the largest number of SEPs identified in plants to date. Our study not only identifies potential lncRNA regulators of plant response to herbivore attack but also serves as a valuable resource for the functional characterization of SEP-encoding lncRNAs.
Functional characterization of a new ORF βV1 encoded by radish leaf curl betasatellite
Authors: Gupta Neha , Reddy Kishorekumar , Gnanasekaran Prabu , Zhai Ying , Chakraborty Supriya , Pappu Hanu R.
Published: September 2022

Whitefly-transmitted begomoviruses infect and damage a wide range of food, feed, and fiber crops worldwide. Some of these viruses are associated with betasatellite molecules that are known to enhance viral pathogenesis. In this study, we investigated the function of a novel βV1 protein encoded by radish leaf curl betasatellite (RaLCB) by overexpressing the protein using potato virus X (PVX)-based virus vector in Nicotiana benthamiana. βV1 protein induced lesions on leaves, suggestive of hypersensitive response (HR), indicating cell death. The HR reaction induced by βV1 protein was accompanied by an increased accumulation of reactive oxygen species (ROS), free radicals, and HR-related transcripts. Subcellular localization through confocal microscopy revealed that βV1 protein localizes to the cellular periphery. βV1 was also found to interact with replication enhancer protein (AC3) of helper virus in the nucleus. The current findings suggest that βV1 functions as a protein elicitor and a pathogenicity determinant.
Nitrogen rates and plant density interactions enhance radiation interception, yield, and nitrogen use efficiencies of maize
Authors: Tian Peiyu , Liu Jiamin , Zhao Yanan , Huang Yufang , Lian Yanhao , Wang Yang , Ye Youliang
Published: September 2022

The contributions of the different leaf layers to maize yields identified as middle leaf > lower leaf > upper leaf, where the vertical photosynthetically active radiation (PAR) in the canopy gradually decreases. We hypothesized that the allocation of more PAR and nitrogen (N) to the highest contributing leaves will would be beneficial for higher yields and N use efficiencies. The N application rate and plant density effectively regulated the canopy light and N distribution. We evaluated the interactive effects of N rate and plant density on the agronomic and ecophysiological characteristics of leaves at different orientations in a 2019/2020 field experiment. In this study, an N application rate of 180 kg ha–1 coupled with a plant density of 82,500 plants ha–1 achieved the highest yield and N recovery efficiency (NRE). In contrast to the traditional farming practices in northern China, the density was increased and N rate was reduced. Densification from 52,500 to 82,500 plants ha–1 increased the population leaf area index (LAI) by 37.1% and total photosynthetically active radiation (TPAR) by 29.2%; however, excessive density (from 82,500 to 97,500 plants ha–1) drastically reduced the proportion of TPAR by 28.0% in the lower leaves. With increased density, the leaf areas and angles of the upper leaves decreased much more than those of the other leaves, which allowed the middle and lower leaves to access more light, which manifested a smaller extinction coefficient for light (KL). A high yield (>1,000 kg ha–1) of maize could be achieved simultaneously with higher NRE; however, it was negatively correlated with internal N use efficiency (IEN). Higher N concentrations and lower total performance index (PItotal) in the lower leaves may be an important rationale for the reduction of IEN in high-yielding maize. Additionally, decreased N rate without yield reduction under higher densities was primarily attributed to the more uniform vertical N distribution [a smaller extinction coefficient for N (KN)]. These results suggest that the N fertilizer rate can be moderately reduced without a reduction in maize yield under high plant densities in northern China.
Integrated management enhances crop physiology and final yield in maize intercropped with blackgram in semiarid South Asia
Authors: Varatharajan T. , Dass Anchal , Choudhary Anil K. , Sudhishri S. , Pooniya V. , Das T. K. , Rajanna G. A. , Prasad Shiv , Swarnalakshmi Karivaradharajan , Harish M. N. , Dhar Shiva , Singh Raj , Raj Rishi , Kumari Kavita , Singh Arjun , Sachin K. S. , Kumar Pramod
Published: September 2022

Photosynthesis, crop health and dry matter partitioning are among the most important factors influencing crop productivity and quality. Identifying variation in these parameters may help discover the plausible causes for crop productivity differences under various management practices and cropping systems. Thus, a 2-year (2019–2020) study was undertaken to investigate how far the integrated crop management (ICM) modules and cropping systems affect maize physiology, photosynthetic characteristics, crop vigour and productivity in a holistic manner. The treatments included nine main-plot ICM treatments [ICM1to ICM4– conventional tillage (CT)-based; ICM5to ICM8– conservation agriculture (CA)-based; ICM9– organic agriculture (OA)-based] and two cropping systems,viz., maize–wheat and maize + blackgram–wheat in subplots. The CA-based ICM module, ICM7resulted in significant (p< 0.05) improvements in the physiological parameters,viz., photosynthetic rate (42.56 μ mol CO2m–2sec–1), transpiration rate (9.88 m mol H2O m–2sec–1) and net assimilation rate (NAR) (2.81 mg cm–2day–1), crop vigour [NDVI (0.78), chlorophyll content (53.0)], dry matter partitioning toward grain and finally increased maize crop productivity (6.66 t ha–1) by 13.4–14.2 and 27.3–28.0% over CT- and OA-based modules. For maize equivalent grain yield (MEGY), the ICM modules followed the trend as ICM7> ICM8> ICM5> ICM6> ICM3> ICM4> ICM1> ICM2> ICM9. Multivariate and PCA analyses also revealed a positive correlation between physiological parameters, barring NAR and both grain and stover yields. Our study proposes an explanation for improved productivity of blackgram-intercropped maize under CA-based ICM management through significant improvements in physiological and photosynthetic characteristics and crop vigour. Overall, the CA-based ICM module ICM7coupled with the maize + blackgram intercropping system could be suggested for wider adoption to enhance the maize production in semiarid regions of India and similar agroecologies across the globe.
Can artificial ecological corridors be used for ecological restoration of cultivated land in Chinese Mollisols?
Authors: Xu HuiBo , Wu SongTao , Diehl Jessica Ann
Published: September 2022

Artisficial ecological corridors (AECs) are internationally recognized as a standard method for restoring the regional ecological environment. However, the coupling relationship between AECs and soil quality has rarely been studied. Harbin, a typical mollisols region in the cold area of China, has severe soil problems and remediation is urgently needed, yet AEC research in this region is lacking. Based on the perspective of soil restoration, the construction factors of ecological corridors are quantitatively evaluated. It can predict the long-term impact of AECs already built along Harbin’s Ashi River on soil chemical indices. This research studied the ecological restoration of secondary woodland, cultivated land within the ecological corridor, and cultivated land outside the influence range of the corridor under the influence of continuous recovery time and different locations in the corridor (distance from the Ashe River). Soil samples were taken from 5 plots, with a total of 161 samples, and 12 indices of soil ecological characteristics were monitored. The result are as follows: It is believed that the quality restoration of mollisols through ecological corridors has great application potential. Based on the low-cost natural restoration of ecological corridors, the highest values of total phosphorus (TP) and soil organic matter (SOM) in soil indices were detected in corridors (restored for more than 10 years). In addition, after ten years of recovery, pH and electrical conductivity (EC) in the ecological corridor returned to normal from high levels in cultivated land that far exceeded the reference values. The recovery process of mollisols mass begins to decrease, then increases, and finally reaches and exceeds the reference value of standard mollisols. The redundancy analysis of soil samples found the distance to be a key factor affecting soil total nitrogen (TN), SOM, and cation exchange capacity (CEC). Recovery time is a crucial factor affecting soil total organic carbon (SOC), pH and EC. According to the TN, SOM, and CEC mollisols indices, the ecological corridor’s unilateral width is 125-150m. According to the SOC, pH, and EC indices of mollisols, the AECs should complete a natural recovery cycle of a minimum of 13 years. This study reveals the change mechanism of soil quality in mollisols area corridors based on recovery time and location. This research offer ideas and a scientific basis for worldwide governments in mollisols to formulate mollisols restoration policies.
Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against Magnaporthe oryzae
Authors: Ninkuu Vincent , Yan Jianpei , Zhang Lin , Fu Zhenchao , Yang Tengfeng , Li Shupeng , Li Beibei , Duan Jiaqi , Ren Jie , Li Guangyue , Yang Xiufen , Zeng Hongmei
Published: September 2022

Magnaporthe oryzae is a potent fungus that adversely affects rice yield. Combinatorial techniques of prevention, toxic chemicals, and fungicide are used to remedy rice blast infection. We reported the role of Hrip1 in cell death elicitation and expression of systematic acquired resistance that could potentially stifle M. oryzae infection. In this study, transcriptome and metabolomic techniques were used to investigate the mechanism by which Hrip1 reprogramed the transcriptome of rice seedlings to confer immunity against M. oryzae. Our results showed that Hrip1 induces cell wall thickening and phytoalexin elicitation to confer immunity against M. oryzae infection. Hrip1 activates key lignin biosynthetic genes and myeloblastosis transcription factors that act as molecular switches for lignin production. Lignin content was increased by 68.46% and more after 48 h onwards in Hrip1-treated seedlings compared to the control treatment. Further analysis of cell wall morphology using the transmission electron microscopy technique revealed over 100% cell wall robustness. Hrip1 also induced the expression of 24 diterpene synthases. These include class I and II terpene synthases, cytochrome P450 subfamilies (OsCYP76M and OsCYP71Z), and momilactones synthases. The relationship between the expression of these genes and metabolic elicitation was analyzed using ultra-performance liquid chromatography–tandem mass spectrometry. Enhanced amounts of momilactones A and B, oryzalactone, and phytocassane A and G were detected in the Hrip1-treated leaves. We also identified seven benzoxazinoid genes (BX1-BX7) that could improve rice immunity. Our findings show that Hrip1 confers dual immunity by leveraging lignin and phytoalexins for physical and chemical resistance. This study provides novel insights into the mechanisms underlying Hrip1-treated plant immunity.
ZAR1: Guardian of plant kinases
Authors: Breit-McNally Clare , Laflamme Bradley , Singh Racquel A. , Desveaux Darrell , Guttman David S.
Published: September 2022

A key facet of innate immunity in plants entails the recognition of pathogen “effector” virulence proteins by host Nucleotide-Binding Leucine-Rich Repeat Receptors (NLRs). Among characterized NLRs, the broadly conserved ZAR1 NLR is particularly remarkable due to its capacity to recognize at least six distinct families of effectors from at least two bacterial genera. This expanded recognition spectrum is conferred through interactions between ZAR1 and a dynamic network of two families of Receptor-Like Cytoplasmic Kinases (RLCKs): ZED1-Related Kinases (ZRKs) and PBS1-Like Kinases (PBLs). In this review, we survey the history of functional studies on ZAR1, with an emphasis on how the ZAR1-RLCK network functions to trap diverse effectors. We discuss 1) the dynamics of the ZAR1-associated RLCK network; 2) the specificity between ZRKs and PBLs; and 3) the specificity between effectors and the RLCK network. We posit that the shared protein fold of kinases and the switch-like properties of their interactions make them ideal effector sensors, enabling ZAR1 to act as a broad spectrum guardian of host kinases.
RETRACTED: Short-term responses of Spinach (Spinacia oleracea L.) to the individual and combinatorial effects of Nitrogen, Phosphorus and Potassium and silicon in the soil contaminated by boron
Authors: Ma Jing , Ali Sajjad , Saleem Muhammad Hamzah , Mumtaz Sahar , Yasin Ghulam , Ali Baber , Al-Ghamdi Abdullah Ahmed , Elshikh Mohamed S. , Vodnar Dan C. , Marc Romina Alina , Rehman Abdur , Khan Muhammad Nauman , Chen Fu , Ali Shafaqat
Published: September 2022

While of lesser prevalence than boron (B) deficient soils, B-rich soils are important to study as they can cause B toxicity in the field and subsequently decrease crop yields in different regions of the world. We have conducted the present study to examine the role of the individual or combined application of silicon (Si) and NPK fertilizer in B-stressed spinach plants (Spinacia oleracea L.). S. oleracea seedlings were subjected to different NPK fertilizers, namely, low NPK (30 kg ha–2) and normal NPK (60 kg ha–2)], which were also supplemented by Si (3 mmol L–1), for varying levels of B in the soil i.e., 0, 250, and 500 mg kg–1. Our results illustrated that the increasing levels of B in the soil caused a substantial decrease in the plant height, number of leaves, number of stems, leaf area, plant fresh weight, plant dry weight, chlorophyll a, chlorophyll b, total chlorophyll, carotenoid content, net photosynthesis, stomatal conductance, transpiration rate, magnesium content in the roots, magnesium contents in the shoots, phosphorus content in the roots, phosphorus content in the leaves in the shoots, iron content in the roots, iron content in the shoots, calcium content in the roots, and calcium content in the shoots. However, B toxicity in the soil increased the concentration of malondialdehyde, hydrogen peroxide, and electrolyte leakage which were also manifested by the increasing activities of enzymatic [superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX)], and non-enzymatic antioxidants (phenolic, flavonoid, ascorbic acid, and anthocyanin content). B toxicity in the soil further increased the concentration of organic acids in the roots such as oxalic acid, malic acid, formic acid, citric acid, acetic acid, and fumaric acid. The addition of Si and fertilizer levels in the soil significantly alleviated B toxicity effects on S. oleracea by improving photosynthetic capacity and ultimately plant growth. The increased activity of antioxidant enzymes in Si and NPK-treated plants seems to play a role in capturing stress-induced reactive oxygen species, as was evident from the lower levels of oxidative stress indicators, organic acid exudation, and B concentration in the roots and shoots of Si and NPK-treated plants. Research findings, therefore, suggested that the Si and NPK application can ameliorate B toxicity in S. oleracea seedlings and result in improved plant growth and composition under metal stress as depicted by the balanced exudation of organic acids.
Cytochrome b5: A versatile electron carrier and regulator for plant metabolism
Authors: Liu Chang-Jun
Published: September 2022

Cytochrome b5 (CB5) is a small heme-binding protein, known as an electron donor delivering reducing power to the terminal enzymes involved in oxidative reactions. In plants, the CB5 protein family is substantially expanded both in its isoform numbers and cellular functions, compared to its yeast and mammalian counterparts. As an electron carrier, plant CB5 proteins function not only in fatty acid desaturation, hydroxylation and elongation, but also in the formation of specialized metabolites such as flavonoids, phenolic esters, and heteropolymer lignin. Furthermore, plant CB5s are found to interact with different non-catalytic proteins such as ethylene signaling regulator, cell death inhibitor, and sugar transporters, implicating their versatile regulatory roles in coordinating different metabolic and cellular processes, presumably in respect to the cellular redox status and/or carbon availability. Compared to the plentiful studies on biochemistry and cellular functions of mammalian CB5 proteins, the cellular and metabolic roles of plant CB5 proteins have received far less attention. This article summarizes the fragmentary information pertaining to the discovery of plant CB5 proteins, and discusses the conventional and peculiar functions that plant CB5s might play in different metabolic and cellular processes. Gaining comprehensive insight into the biological functions of CB5 proteins could offer effective biotechnological solutions to tailor plant chemodiversity and cellular responses to environment stimuli.
Acrophialophora jodhpurensis: an endophytic plant growth promoting fungus with biocontrol effect against Alternaria alternata
Authors: Daroodi Zoha , Taheri Parissa , Tarighi Saeed
Published: September 2022

In this study, efficiency of the endophytic fungal isolate Msh5 was evaluated on promoting tomato plant growth and controllingAlternaria alternata, the causal agent of early blight in tomatoes. Morphological and molecular (ITS and tub2 sequences) analyses revealed that the fungal isolate, Msh5, wasAcrophialophora jodhpurensis(Chaetomium jodhpurenseLodha). This beneficial fungus was capable of producing indole-3-acetic acid (IAA), urease, siderophore, extracellular enzymes, and solubilized phosphate. Under laboratory conditions, the Msh5 isolate ofA. jodhpurensisinhibitedA. alternatagrowth in dual culture, volatile and non-volatile metabolites assays. The supernatant of this endophytic fungus was capable of reducing spore germination and altering the hyphal structure ofA. alternataand the spores produced germ tubes showed vacuolization and abnormal structure compared to the control. Also, the effect ofA. jodhpurensison plant growth parameters (such as shoot and root weight and length) and suppressingA. alternatawas investigatedin vivovia seed inoculation with spores ofA. jodhpurensisusing 1% sugar, 0.5% carboxymethyl cellulose (CMC) or 0.5% molasses solution as stickers. Colonization of tomato roots by the endophytic fungus resulted in significant increasing plant growth parameters and reduction in the progress of the diseases caused byA. alternatacompared to the controls. Among the different coating materials used as stickers, sugar was found to be the most effective for enhancing plant growth parameters and decreasing the disease progress. Therefore,A. jodhpurensisisolate Msh5 can be suggested as a potential biofertilizer and biocontrol agent for protecting tomato plants againstA. alternata.
Comparative physiological analyses and the genetic basis reveal heat stress responses mechanism among different Betula luminifera populations
Authors: Hu Xian-Ge , Xu Yilei , Shen Ning , Liu Mingtong , Zhuang Hebi , Borah Priyanka , Tong Zaikang , Lin Erpei , Huang Huahong
Published: September 2022

Betula luminifera is a subtropical fast-growing timber species with high economic value. However, along with global warming, heat stress become one of the main environmental variables that limit the productivity of B. luminifera, and the response of diverse geographic populations to high temperatures is still unclear. In order to offer a comprehensive understanding of the behavior of B. luminifera under heat stress, the physiological responses of six B. luminifera populations (across the core distribution area) were described in this work in an integrated viewpoint. The results showed that a multi-level physiological regulatory network may exist in B. luminifera, the first response was the activity of resistant enzymes [e.g., peroxidase (POD)] at a preliminary stage of 2 h heat stress, and then the proline (osmoregulation substance) content began to increase after 24 h of continuous high-temperature treatment. In addition, photosynthesis was stronlgly affected by heat stress, and the net photosynthetic rate (Pn) showed a downward trend under heat treatment in all six B. luminifera populations. Interestingly, although the physiological change patterns of the six B. luminifera populations were relatively consistent for the same parameter, there were obvious differences among different populations. Comprehensive analysis revealed that the physiological response of Rongshui (RS) was the most stable, and this was the representative B. luminifera population. Illumina RNA-seq analysis was applied to reveal the specific biological process of B. luminifera under heat stress using the RS population, and a total of 116,484 unigenes were obtained. The differentially expressed genes (DEGs) between different time periods under heat stress were enriched in 34 KEGG pathways, and the limonene and pinene degradation pathway was commonly enriched in all pairwise comparisons. Moreover, transcription factors including bHLH (basic helix–loop–helix), MYB, WRKY, and NAC (NAM, ATAF1/2, and CUC2) were identified. In this study, the physiological response and tolerance mechanisms of B. luminifera under high temperature stress were revealed, which can conducive to the basis of B. luminifera selection and resistance assessment for cultivation and breeding.
Labor market situation of refugees in Europe: The role of individual and contextual factors
Authors: Kosyakova Yuliya , Kogan Irena
Published: September 2022

The paper sheds light on the patterns of labor market integration of refugees in Western countries, who arrived primarily during the 2015–2016 mass refugee migration. Its major focus lies on the role of individual and contextual factors responsible for refugees' success in the labor market. At the host country level, the extent of permeability along the ethnic lines and the welcome of reception—both on the part of the majority population and the part of the established minorities—constitute further essential moderators of refugees' labor market success. This comprehensive literature overview draws on the flourishing body of research in Europe and beyond and discusses commonalities and differences across refugee origins and destinations while paying particular attention to the time trends and meaningful heterogeneities along with refugees' socio-demographic characteristics. We conclude by identifying major avenues for future research.
How did lockdown and social distancing policies change the eating habits of diabetic patients during the COVID-19 pandemic? A systematic review
Authors: Lashkarbolouk Narges , Mazandarani Mahdi , Pourghazi Farzad , Eslami Maysa , Khonsari Nami Mohammadian , Ghonbalani Zahra Nouri , Ejtahed Hanieh-Sadat , Qorbani Mostafa
Published: September 2022

Background

After the declaration of the COVID-19 pandemic, governments established national lockdowns and social distancing as an effective plan to control this disease. As a result of the lockdown policies, diabetic patients` access to food products, medication, and routine follow-ups is disrupted, making it difficult for them to control their disease.

Methods

International databases, including PubMed/Medline, Web of Science, and Scopus, were searched until April 2022. All observational studies included assessing the impact of lockdown and social distancing on eating habits (as primary outcome), and glycemic and anthropometric indices (as secondary outcomes) of diabetic patients during the COVID-19 pandemic. The Newcastle-Ottawa Quality Scale was used to assess the quality rating of the studies.

Results

Overall, 22 studies were included in this systematic review, the results of which varied in different communities. In most studies, consumption of grains, fruits, and vegetables was reported to increase. On the other hand, consumption of snacks and sweets was reported to increase in other surveys. During the COVID-19 lockdown, most diabetic patients preferred to cook meals at home, using less takeout, fast foods, and alcoholic drinks. Although the patients mostly improved their eating habits, the glycemic and anthropometric indices were contradictory in different studies. Studies showed that the eating habits of diabetic patients vary from country to country, even in some cases and studies done in the same country showed different results. For example, all the studies done in Japan showed an increase in the consumption of snacks and sweets, leading to weight gain in the patients. However, conflicting results in eating habits have been observed in studies conducted in India.

Conclusion

The lockdown policies have led to a beneficial change in the eating habits of diabetic patients to consume more fruits and vegetables and reduce the consumption of animal protein products and alcoholic beverages. While some diabetic patients have increased consumption of snacks and sweets, leading to a disturbance in their glycemic and anthropometric indices control. Understanding the consequences of lockdown and social distancing of the diabetic patient during the COVID-19 pandemic can help public health authorities make better recommendations to improve glycemic control.
Modeling the relationship between perceived service quality, tourist satisfaction, and tourists’ behavioral intentions amid COVID-19 pandemic: Evidence of yoga tourists’ perspectives
Authors: Abdou Ahmed Hassan , Mohamed Shaimaa Abo Khanger , Khalil Ayman Ahmed Farag , Albakhit Azzam Ibrahem , Alarjani Ali Jukhayer Nader
Published: September 2022

Purpose

This study aims to investigate the impact of perceived service quality (PSQ) on tourist satisfaction and behavioral intentions and explore the potential mediating role of tourist satisfaction in the relationship between service quality and behavioral intentions in the yoga tourism context during the COVID-19 pandemic. Further, this is to examine to what extent yoga tourist satisfaction directly affects their behavioral intentions.

Design/methodology/approach

Based on a review of literature, the study proposes a conceptual model to test four hypothesized relationships among the constructs of perceived service quality, tourist satisfaction, and behavioral intentions. Data was collected by using a self-administrated questionnaire that was developed and directed to a convenience sample of yoga tourists (380 forms). Structural equation modeling (SEM) was employed to determine the relationship between study constructs.

Findings

The results of SEM illustrated that all the hypothesized relationships are supported. The findings confirm that yoga tourists’ behavioral intentions are significantly affected directly and indirectly (through tourist satisfaction) by perceived service quality. Additionally, tourist satisfaction significantly partially mediates the relationship between PSQ and tourists’ behavioral intentions.

Research limitations

The subject of this study was yoga tourists staying in yoga retreats/studios in Egyptian destinations (South Sinai Governorate). Future research may focus on other geographical destinations and other influential variables of yoga tourists’ satisfaction and behavioral intentions should be investigated.

Practical implications

For improving tourists’ satisfaction and behavioral intentions, yoga service providers should take care by giving tourists personalized attention, and understanding, fulfilling their specific needs. Health and hygiene practices must be considered during the COVID-19 pandemic.

Originality/value

This study is perhaps the first empirical study that examines the relationship between PSQ and tourists’ satisfaction and behavioral intentions in the yoga tourism context. A new integrated conceptual model that combined three service quality dimensions, namely, tangibles, intangibles as well as health and hygiene was developed and validated.