bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between Social Media Use and Social Anxiety in College Students: Mediation Effect of Communication Capacity
Authors: Lai Fengxia , Wang Lihong , Zhang Jiyin , Shan Shengnan , Chen Jing , Tian Li
Published: February 2023

An increasing number of college students are experiencing social anxiety in an era of prevalent social networking. College students’ social anxiety may be related to their social media use. However, this relationship has not been confirmed. This study aimed to investigate the relationships between different types of social media use and social anxiety among college students, and the mediation effects of communication capacity in this context. A large sample of 1740 students from seven colleges in China was analyzed. Bivariate correlation and structural equations analysis showed that passive social media use was positively correlated with social anxiety. Active social media use was negatively correlated with social anxiety. Communication capacity partially mediated the relationship between social media use (passive/active) and social anxiety. Active social media use may reduce social anxiety by positively mediating communication capacity, while improved communication capacity may reduce the contribution of passive use to social anxiety. The differences in the effects of different social media use on social anxiety deserve the attention of educators. Developing communication capacity education around college students may help reduce their social anxiety.
Workplace Health Promotion Embedded in Medical Surveillance: The Italian Way to Total Worker Health Program
Authors: Magnavita Nicola
Published: February 2023

In 2011, NIOSH launched the Total Worker Health (TWH) strategy based on integrating prevention and health promotion in the workplace. For several years now, in Italy, this integration has led to the creation of workplace health promotion embedded in medical surveillance (WHPEMS). WHPEMS projects, which are also implemented in small companies, focus each year on a new topic that emerges from the needs of workers. During their regular medical check-up in the workplace, workers are invited to fill in a questionnaire regarding the project topic, its outcome, and some related factors. Workers receive advice on how to improve their lifestyles and are referred to the National Health Service for any necessary tests or treatments. Results collected over the past 12 years from more than 20,000 participants demonstrate that WHPEMS projects are economical, sustainable, and effective. The creation of a network of occupational physicians who are involved in WHPEMS projects could help to improve the work culture, health, and safety of workers.
Comparison of Quality of Life in Breast Cancer Survivors with and without Persistent Depressive Symptoms: A 12-Month Follow-Up Study
Authors: Ribeiro Fernanda , Tebar William , Ferrari Gerson , Palma Mariana , Fregonesi Cristina , Caldeira Daniela , Silva Gabriela , Vanderlei Luiz , Beretta Victor , Christofaro Diego
Published: February 2023

Although breast cancer treatments reduce mortality, their adverse effects can increase depression which impacts one’s quality of life (QoL). Physical activity (PA) seems to improve the QoL of breast cancer survivors (BCS). However, an unanswered question is the influence of PA on the QoL in BCS with depressive symptoms. Thus, we analyzed the influence of PA on the QoL in BCS with persistent depressive symptoms during 12 months of follow-up. The sample included 70 female BCS. Depression and QoL domains (i.e., functional capacity, physical limitations, body pain, general health status, vitality, social and emotional aspects, and mental health) were assessed at baseline and follow-up periods by the Hospital Anxiety and Depression Scale and SF-36, respectively. Habitual PA was assessed by Baecke’s questionnaire. Our results indicate a prevalence of 17.1% of depressive symptoms. Non-depressives BCS improved their physical limitations and general health status domains over time, but there were no observed differences in depressive BCS. BCS with persistent depressive symptoms (baseline and follow-up) showed worse QoL scores than non-depressives in all domains, regardless of confounding factors. When adjusted for PA, the difference between BCS depressives and non-depressives lost its significance in the functional capacity domain. In conclusion, habitual PA practice positively influenced the functional capacity domain of the QoL in BCS.
Burnout among Residents: Prevalence and Predictors of Depersonalization, Emotional Exhaustion and Professional Unfulfillment among Resident Doctors in Canada
Authors: Shalaby Reham , Oluwasina Folajinmi , Eboreime Ejemai , El Gindi Hany , Agyapong Belinda , Hrabok Marianne , Dhanoa Sumeet , Kim Esther , Nwachukwu Izu , Abba-Aji Adam , Li Daniel , Agyapong Vincent Israel Opoku
Published: February 2023

Background: Burnout in the medical profession has garnered a lot of attention over recent years. It has been reported across all specialties and all stages of medical education; however, resident doctors in particular are at risk for burnout throughout their years of training. This study was aimed at evaluating the prevalence and correlates of burnout among resident doctors in Alberta. Methods: Through a descriptive cross-sectional study design, a self-administered questionnaire was used to gather data from resident doctors at two medical schools in Alberta, Canada. The Maslach Burnout Inventory was used as the assessment tool. Chi-squared and multivariate binary logistic regression analyses were used. Results: Overall burnout prevalence among residents was 58.2%, and for professional fulfilment index, it was 56.7% for work exhaustion and interpersonal disengagement and 83.5% for lack of professional fulfillment. Working more than 80 h/week (OR = 16.437; 95% CI: 2.059–131.225), being dissatisfied (OR = 22.28; 95% CI: 1.75–283.278) or being neither satisfied nor dissatisfied with a career in medicine (OR = 23.81; 95% CI: 4.89–115.86) were significantly associated with high depersonalization. Dissatisfaction with efficiency and resources (OR = 10.83; CI: 1.66–70.32) or being neither satisfied nor dissatisfied with a career in medicine (OR = 5.14; CI: 1.33–19.94) were significantly associated with high emotional exhaustion. Working more than 80 h/week (OR = 5.36; CI: 1.08–26.42) and somewhat agreeing that the residency program has enough strategies aimed at resident well-being in place (OR = 3.70; CI: 1.10–12.46) were significantly associated factors with high work exhaustion and interpersonal disengagement. A young age of residents (≤30 years) (OR = 0.044; CI: 0.004–0.445) was significantly associated with low professional fulfillment. Conclusion: Burnout is a serious occupational phenomenon that can degenerate into other conditions or disrupt one’s professional performance. Significant correlates were associated with high rates of burnout. Leaders of medical schools and policymakers need to acknowledge, design, and implement various strategies capable of providing continuous effective mental health support to improve the psychological health of medical residents across Canada.
Effect of Coal Mining on Soil Microorganisms from Stipa krylovii Rhizosphere in Typical Grassland
Authors: Xie Linlin , Bi Yinli , Zhang Yanxu , Guo Nan
Published: February 2023

The environmental changes caused by coal mining activities caused disturbances to the plant, soil, and microbial health in the mining area. Arbuscular mycorrhizal fungi (AMF) play an important role in the ecological restoration of mining areas. However, it is less understood how soil fungal communities with multiple functional groups respond to coal mining, and the quantitative impact and risk of mining disturbance. Therefore, in this study, the effect of coal mining on soil microorganisms’ composition and diversity were analyzed near the edge of an opencast coal-mine dump in the Shengli mining area, Xilingol League, Inner Mongolia. The response strategy of soil fungi to coal mining and the stability of arbuscular mycorrhizal fungi (AMF) in the soil fungal community were determined. Our results showed that coal mining affected AMF and soil fungi in areas within 900 m from the coal mine. The abundance of endophytes increased with the distance between sampling sites and the mine dump, whereas the abundance of saprotroph decreased with the distance between sampling sites and the mine dump. Saprotroph was the dominant functional flora near the mining area. The nodes percentage of Septoglomus and Claroideoglomus and AMF phylogenetic diversity near the mining area were highest. AMF responded to the mining disturbance via the variety and evolution strategy of flora. Furthermore, AMF and soil fungal communities were significantly correlated with edaphic properties and parameters. Soil available phosphorus (AP) was the main influencer of soil AMF and fungal communities. These findings evaluated the risk range of coal mining on AMF and soil fungal communities and elucidated the microbial response strategy to mining disturbance.
The Role of Magnetic Transcranial Stimulation in the Diagnosis and Post-Surgical Follow-Up of Cervical Spondylotic Myelopathy
Authors: Vázquez-Sánchez Fernando , Lloria-Gil María del Carmen , Gómez-Menéndez Ana Isabel , Isidro-Mesas Francisco , Echavarría-Íñiguez Ana , Martín-Alonso Javier , González-Bernal Jerónimo , González-Santos Josefa , Berardi Anna , Tofani Marco , Galeoto Giovanni , García-López Beatriz
Published: February 2023

Degenerative cervical myelopathy (DCM) consists of spinal cord damage due to its compression through the cervical spine. The leading cause is degenerative. The diagnosis is clinical, and the therapeutic approach is usually surgical. Confirmation of the diagnostic suspicion is done by magnetic resonance imaging (MRI); however, this test lacks functional information of the spinal cord, the abnormality of which may precede involvement in neuroimaging. Neurophysiological examination using somatosensory evoked potentials (SSEPs) and transcranial magnetic stimulation (TMS) allows for an evaluation of spinal cord function, and provides information in the diagnostic process. Its role in the post-surgical follow-up of patients undergoing decompressive surgery is being studied. We present a retrospective study of 24 patients with DCM and surgical decompression who underwent neurophysiological tests (TMS and SSEP) before, 6, and 12 months after surgery. The result of the TMS and the SSEP in the post-operative follow-up did not correlate with the clinical outcome, either subjective or measured by clinical scales at six months. We only found post-surgical improvement of central conduction times (CMCTs) in patients with severe pre-surgical motor impairment on TMS. In patients with normal pre-surgical CMCT, we found a transient worsening with return to baseline at the one-year follow-up. Most patients presented pre-surgical increased P40 latency at diagnosis. CMCT and SSEP were more related to clinical outcomes one year after the surgical procedure and were very useful in diagnosing.
Burnout Syndrome in Selectable Athletes for the Brazilian Handball Team—Children Category
Authors: Santos-Afonso Max dos , Lourenção Luciano Garcia , Afonso Marla dos Santos , Saes Mirelle de Oliveira , Santos Fernando Braga dos , Penha José Gustavo Monteiro , Galvão Daniela Menezes , Ximenes Neto Francisco Rosemiro Guimarães , Sasaki Natalia Sperli Geraldes Marin dos Santos , Santos Maria de Lourdes Sperli Geraldes , Borges Flávio Adriano , Oliveira Jacqueline Flores de , Rodrigues Sidiane Teixeira , Bandeira Eliel de Oliveira , Alcantara Francisco Cavalcante de , Cunha Carlos Leonardo Figueiredo , Silva Francielle Garcia da , Lemos Messias , Soares Junior Abelardo de Oliveira , Neves Fernanda Burlani
Published: February 2023

To investigate the presence of burnout syndrome in child athlete tryouts for the Brazilian Handball Team, before and after the National Development and Technical Improvement Camp is of great interest. A correlational study, with longitudinal design of the before-and-after type, carried out with 64 male athletes in the children’s category, immersed in the National Camp for Development and Improvement of Handball Technique, in the municipality of São Bernardo do Campo, São Paulo, Brazil, in December 2018. To evaluate burnout syndrome, we used the Athlete Burnout Questionnaire (ABQ). There was a statistically significant increase of the mean scores for burnout and dimensions (Physical and Emotional Exhaustion = 1.5 to 1.6; p-value < 0.001; Reduced Sense of Accomplishment = 2.7 to 2.9; p-value < 0.001; Sports Devaluation = 1.4 to 1.6; p-value < 0.001; and General Burnout = 1.9 to 2.0; p-value < 0.001). The athletes selected for the national team had lower mean scores for general burnout and dimensions (Physical and Emotional Exhaustion = 1.5; Reduced Sense of Accomplishment = 2.7; Sports Devaluation = 1.5; General Burnout = 1.9). The National Camp for Development and Technical Improvement can have a negative impact on the mental health of athletes. This event is important to select the competitors with greater ability to face the pressure and adversities present in the sport environment.
Cross-Cultural Adaptation and Validation of the Serbian Version of the Brief Assessment of Cognition in Schizophrenia Scale
Authors: Poznanovic Sanja Totic , Markovic Milos , Stasevic Milena , Karlicic Ivana Stasevic , Tomanic Milena
Published: February 2023

The Brief Assessment of Cognition in Schizophrenia (BACS) scale was developed for the assessment of cognitive function in patients with schizophrenia. The objective of this study was to cross-culturally adapt and validate the BACS in to the Serbian language. The study was conducted at the Laza Lazarevic Clinic for Mental Disorders and the Clinic for Psychiatry of the University Clinical Center of Serbia from March 2021 to January 2022. The study enrolled 61 inpatients diagnosed with schizophrenia and 61 healthy controls matched for age and sex. Compared with the healthy control group, the schizophrenia patient group had worse cognitive function in all dimensions measured using BACS (p < 0.001 for all measures). The mean standardized composite BACS score was z = −2.46, and symbol coding (z = −2.54) was the most deficient function. Principal component analysis suggests a two-factor structure, where the first factor consisted of loading the measures of verbal and working memory, attention, speed of information processing, and executive function, while the second factor regarded the loading of motor speed. Cronbach’s alpha coefficient demonstrated an excellent level of internal consistency (0.798). These outcomes suggest that the Serbian BACS neurocognitive battery’s psychometric properties are satisfactory, with good overall discriminant validity and high internal consistency. The Serbian BACS appears to be a quick and reliable neuropsychological instrument for evaluating global cognition in schizophrenia patients in Serbia.
Changes of Oral and Physical Functions in Older Japanese Who Participated in Keyboard Harmonica and Exercise Classes during COVID-19-Related Movement Restrictions
Authors: Mizutani Shinsuke , Matsuzaki Hideaki , Iyota Kiyomi , Tani Asuka , Oku Saori , Tabuchi Hiroaki , Fujiwara Akiko , Hase-Tamaru Shizuka , Kishimoto Hiro , Narazaki Kenji
Published: February 2023

Many older people have restricted activities or movements because of the coronavirus disease 2019 (COVID-19) pandemic, which causes concerns about secondary health problems. This study aimed to investigate how frailty-prevention activities implemented by local governments have changed the health of community-dwelling older people during the COVID-19 pandemic. In this observational study, the participants were 23 older Japanese people who took part in keyboard harmonica or exercise classes in 2021. Oral function examination and physical function tests were conducted at baseline and after 10 months of follow-up. In each class, the participants met 15 times and worked on assignments at home. The results showed that oral diadochokinesis/pa/, which represents lip dexterity, improved during 10 months (from 6.6 to 6.8 times/s, p < 0.046); however, grip strength (p < 0.005) and total skeletal muscle mass (p < 0.017) decreased in the keyboard harmonica group. In the exercise group, a statistically significant difference was found only in grip strength, which decreased (p < 0.003). The oral and physical functions of older people who participated in frailty-prevention activities implemented by local governments characteristically changed. Moreover, activity restrictions during the COVID-19 pandemic may have caused decreased grip strength.
Special Issue: Sport Psychology Interventions for Athletes’ Performance and Well-Being
Authors: di Fronso Selenia , Budnik-Przybylska Dagmara
Published: February 2023

Scientific evidence highlights that sport psychology interventions adopted by professionals are crucial for making a difference in athlete performance [...]
Effectiveness of the Ponseti Method in the Treatment of Clubfoot: A Systematic Review
Authors: López-Carrero Elena , Castillo-López José Manuel , Medina-Alcantara Miguel , Domínguez-Maldonado Gabriel , Garcia-Paya Irene , Jiménez-Cebrián Ana María
Published: February 2023

Clubfoot is a common congenital deformity of the lower limbs. It should be treated as soon as possible so that its correction is more easily achieved. The objective of this systematic review was to assess the effectiveness of the Ponseti method in the treatment of clubfoot. A bibliographic search was carried out in different databases, including PubMed and SciELO. Filters such as full text and randomized controlled trial were selected to find those articles that best matched our search. Among the results, we selected the ones that interested us, and the rest were discarded, either because they did not meet the requirements for our work or because they were repeated. In total, we collected 19 articles, but after using the critical evaluation instrument CASPe, 7 of them were eliminated, leaving us with a total of 12 articles for our systematic review. After analyzing the results obtained in the selected articles, we concluded that the Ponseti method is effective in the treatment of clubfoot, presenting a high success rate.
Factors Influencing Spiritual Health among Nursing Students in the Prolonged COVID-19 Situation
Authors: Jin Juhyun
Published: February 2023

The COVID-19 pandemic is not only an epidemiological crisis but also a spiritual health crisis that affects nursing students. Spiritual health is essential in maintaining and promoting physical and mental health to achieve happiness, potential, meaning, and purpose of life even during a pandemic. This descriptive cross-sectional study aimed to examine factors affecting spiritual health of nursing college students. The study adheres to the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines. A total of 219 nursing students from three nursing colleges in Metropolitan D city participated in the study through an online Google Form questionnaire from 2–18 September 2021. The mean score of spiritual health was 96.98 ± 11.54 (out of 120 points); spiritual health was significantly positively correlated with life satisfaction and academic performance (p < 0.001) and negatively correlated with academic stress (p < 0.001). Factors significantly affecting spiritual health were academic stress (ß = −2.21, p = 0.045), life satisfaction (ß = 3.85, p < 0.001), and academic performance; below score of 3.0 (ß = −2.08, p = 0.039). The explanatory power of these effects was 30.7%. As a future professional nurse who will work in the clinical field where the demand for the spiritual care of patients is increasing, it is necessary to develop and apply a curriculum that can improve the spiritual health of nursing students.
Genetic Risk Scores for the Determination of Type 2 Diabetes Mellitus (T2DM) in North India
Authors: Shitomi-Jones Lisa Mitsuko , Akam Liz , Hunter David , Singh Puneetpal , Mastana Sarabjit
Published: February 2023

Background: Globally, type 2 diabetes mellitus (T2DM) is one of the fastest-growing noncommunicable multifactorial and polygenic diseases, which leads to many health complications and significant morbidity and mortality. South Asians have a high genetic predisposition to T2DM, with India being home to one in six diabetics. This study investigates the association of selected genetic polymorphisms with T2DM risk and develops a polygenic risk score (PRS). Methods: A case–control study recruited fully consented participants from a population of Jat Sikhs in north India. DNA samples were genotyped for a range of polymorphisms and odds ratios were calculated under several genetic association models. Receiver operating characteristic (ROC) curves were produced for combinations of the PRS and clinical parameters. Results: The GSTT1(rs17856199), GSTM1(rs366631), GSTP1(rs1695), KCNQ1(rs2237892), ACE(rs4646994), and TCF7L2(rs12255372; rs7903146; rs7901695) polymorphisms were associated with increased T2DM risk (p ≤ 0.05). No association was observed with IGF2BP2(rs4402960) or PPARG2(rs1801282). The weighted PRS was found to be significantly higher in patients (mean = 15.4, SD = 3.24) than controls (mean = 11.9, SD = 3.06), and t(454) = −12.2 (p < 0.001). The ROC curve analysis found the weighted PRS in combination with clinical variables to be the most effective predictor of T2DM (area under the curve = 0.844, 95%CI = 0.0.808–0.879). Conclusions: Several polymorphisms were associated with T2DM risk. PRS based on even a limited number of loci improves the prediction of the disease. This may provide a useful method for determining T2DM susceptibility for clinical and public health applications.
The Effectiveness of an Empowerment Education Intervention for Substance Use Reduction among Inner-City Adolescents in Nigeria
Authors: Ojonuba Hassana Shuaibu , Abdul Rahman Haliza , Zaremohzzabieh Zeinab , Mohd Zulkefli Nor Afiah
Published: February 2023

(1) Background: Substance use among inner-city adolescents is at an alarming rate in Nigeria. Despite their high exposure to this risk, limited experimental tests have been conducted on prevention programs. (2) Methods: This study investigates the effectiveness of an empowerment education intervention in reducing the risk of substance use in Abuja’s inner-city adolescents. Random selection placed adolescents into intervention and control conditions, and assessment was conducted at baseline, post-test, and 3-months follow-up intervention. After pre-test, the intervention group engaged in an empowerment education intervention of 11 sessions. (3) Results: In a post-test of three months, results show significant and positive changes among adolescents in substance use, including a notable reduction in positive attitudes toward drugs. In other words, the results showed adolescents reported less depression and substance use as well as higher peer support, parental support, social competence, and self-esteem at post-test and 3-month follow-ups as compared to the pre-intervention period. In addition, at both post-test and the 3-month follow-up, the intervention group performed better than the control group on peer support, parental support, social competence, and self-esteem. (4) Conclusions: This study presents a new indication that the empowerment education intervention effectively reduces substance use among Nigeria’s inner-city adolescents.
Did the COVID-19 Pandemic Restrict Access to Emergency Urological Services: Assessment of Reorganisation Effectiveness for Hospital Treatment
Authors: Kaczmarek Krystian , Kalembkiewicz Jakub , Jankowska Marta , Kalembkiewicz Karolina , Narożnicki Jakub , Lemiński Artur , Słojewski Marcin
Published: February 2023

Urolithiasis derived renal colic is a common urological condition. If treated properly, the disease resolves without complications; if not treated, it causes infection and renal failure. The COVID-19 restrictions impacted hospitalised treatment of diseases. We analysed the impact of COVID-19 on renal colic treatment at a hospital in Poland. Clinical and demographic data of patients treated during the COVID-19 era were compared with those treated before this pandemic. During the COVID-19 restrictions, renal colic patient hospital admissions fell significantly. However, more patients presented with chronic renal colic symptoms and urinary tract infections. Nevertheless, the degree of hydronephrosis and the number and location of stones did not differ between the two groups. No marked changes were observed in the chosen treatment options. The observed decrease in emergency admissions of patients with acute renal colic with a simultaneous increase in the rate of infectious stones might indicate that some patients requiring urgent medical help did not report to the emergency department or came later than they would before the pandemic, reporting more serious symptoms. One plausible explanation for this may be that the reorganisation of the healthcare system restricted access to urological care. Moreover, some patients may have delayed their visit to the hospital due to the fear of contracting the SARS-CoV-2 coronavirus.
The Nexus of Sports-Based Development and Education of Mental Health and Physical Fitness
Authors: Zhang Tiejun , Liu Huarong , Lu Yi , Wang Qinglei
Published: February 2023

Physical inactivity has increased globally, particularly in developed nations. A high proportion of the human population is unable to meet the physical activity recommendation of the World Health Organisation due to hypertension, metabolic syndrome, obesity, and other medical conditions. Non-communicable diseases and mental health problems are becoming more prevalent, particularly in low and middle-income nations. This study aimed to determine the effectivenessof a mentorship programmeon university students’ mental health and physical fitness. The intervention comprised the effects of sports-based development and education on physical fitness and mental health. A total of 196 and 234 students from two universities were randomly assigned to the intervention and control groups, respectively. The primary outcomes were engagement in physical activities (number of push-ups for 1 min, the strength of hand grip (kg), and the Jump test while standing (cm)), body fat proportion and psychological resilience, self-efficacy, and relationship with family and schoolmates. Participants in the control group had access to a web-based health education game, whereas the intervention group wassubjected to intensive interventional activities for one month based on the eight principles of the National Research Council and Institute of Medicine. Data were analysed using Analysis of Variance (ANOVA) to compare the physical and mental components between the intervention and control groups. Relative to baseline, all the physical health components (push-ups, sit-ups, and jump tests), psychological resilience, relationship with family members, and self-efficacy increased significantly in the intervention compared to the control group. Body fat composition was significantly reduced in the intervention when compared tothe control group. In conclusion, the mentorship programme effectively improved the participants’ physical and psychological health and could be developed further for application in a larger population.
Second-Child Fertility Intentions among Urban Women in China: A Systematic Review and Meta-Analysis
Authors: Yang Yu , He Rongxin , Zhang Ning , Li Liming
Published: February 2023

With the adjustment of China’s fertility policy, the topic of women’s fertility has attracted much attention. In particular, urban women face a difficult choice between family and work. This study analyzed the prevalence and determinants of second-child fertility intention among urban women in China and aimed to provide evidence for improving fertility rate measures. A systematic review and meta-analysis were conducted using quantitative primary studies. We identified 16 cross-sectional studies that investigated a total of 24,979 urban women. The prevalence of second-child fertility intentions was 37%. A subgroup analysis revealed that the highest prevalence was observed between 2016 and 2017, and the lowest was observed in first-tier cities. Meta-analyses indicated that 18 factors were significantly associated with second-child fertility intentions, including demographic factors, fertility attitude, husbands, children, parents, or others. The findings of this study highlight the low second-child fertility intentions among urban women in China. Therefore, policymakers should consider various aspects, gradually optimizing fertility-supporting facilities, while encouraging fertility.
Urban Growth Forecast Using Machine Learning Algorithms and GIS-Based Novel Techniques: A Case Study Focusing on Nasiriyah City, Southern Iraq
Authors: Hanoon Sadeq Khaleefah , Abdullah Ahmad Fikri , Shafri Helmi Z. M. , Wayayok Aimrun
Published: February 2023

Land use and land cover changes driven by urban sprawl has accelerated the degradation of ecosystem services in metropolitan settlements. However, most optimisation techniques do not consider the dynamic effect of urban sprawl on the spatial criteria on which decisions are based. In addition, integrating the current simulation approach with land use optimisation approaches to make a sustainable decision regarding the suitable site encompasses complex processes. Thus, this study aims to innovate a novel technique that can predict urban sprawl for a long time and can be simply integrated with optimisation land use techniques to make suitable decisions. Three main processes were applied in this study: (1) a supervised classification process using random forest (RF), (2) prediction of urban growth using a hybrid method combining an artificial neural network and cellular automata and (3) the development of a novel machine learning (ML) model to predict urban growth boundaries (UGBs). The ML model included linear regression, RF, K-nearest neighbour and AdaBoost. The performance of the novel ML model was effective, according to the validation metrics that were measured by the four ML algorithms. The results show that the Nasiriyah City expansion (the study area) is haphazard and unplanned, resulting in disastrous effects on urban and natural systems. The urban area ratio was increased by about 10%, i.e., from 2.5% in the year 1992 to 12.2% in 2022. In addition, the city will be expanded by 34%, 25% and 19% by the years 2032, 2042 and 2052, respectively. Therefore, this novel technique is recommended for integration with optimisation land use techniques to determine the sites that would be covered by the future city expansion.
Association between Microorganisms and Microplastics: How Does It Change the Host–Pathogen Interaction and Subsequent Immune Response?
Authors: Yang Wenjie , Li Yang , Boraschi Diana
Published: February 2023

Plastic pollution is a significant problem worldwide because of the risks it poses to the equilibrium and health of the environment as well as to human beings. Discarded plastic released into the environment can degrade into microplastics (MPs) due to various factors, such as sunlight, seawater flow, and temperature. MP surfaces can act as solid scaffolds for microorganisms, viruses, and various biomolecules (such as LPS, allergens, and antibiotics), depending on the MP characteristics of size/surface area, chemical composition, and surface charge. The immune system has efficient recognition and elimination mechanisms for pathogens, foreign agents, and anomalous molecules, including pattern recognition receptors and phagocytosis. However, associations with MPs can modify the physical, structural, and functional characteristics of microbes and biomolecules, thereby changing their interactions with the host immune system (in particular with innate immune cells) and, most likely, the features of the subsequent innate/inflammatory response. Thus, exploring differences in the immune response to microbial agents that have been modified by interactions with MPs is meaningful in terms of identifying new possible risks to human health posed by anomalous stimulation of immune reactivities.
Study on the Potential for Stimulating Mulberry Growth and Drought Tolerance of Plant Growth-Promoting Fungi
Authors: Ou Ting , Zhang Meng , Gao Haiying , Wang Fei , Xu Weifang , Liu Xiaojiao , Wang Li , Wang Ruolin , Xie Jie
Published: February 2023

Drought stress often leads to heavy losses in mulberry planting, especially for fruits and leaves. Application of plant growth-promoting fungi (PGPF) endows various plant beneficial traits to overcome adverse environmental conditions, but little is known about the effects on mulberry under drought stress. In the present study, we isolated 64 fungi from well-growing mulberry trees surviving periodical drought stress, and Talaromyces sp. GS1, Pseudeurotium sp. GRs12, Penicillium sp. GR19, and Trichoderma sp. GR21 were screened out due to their strong potential in plant growth promotion. Co-cultivation assay revealed that PGPF stimulated mulberry growth, exhibiting increased biomass and length of stems and roots. Exogenous application of PGPF could alter fungal community structures in the rhizosphere soils, wherein Talaromyces was obviously enhanced after inoculation of Talaromyces sp. GS1, and Peziza was increased in the other treatments. Moreover, PGPF could promote iron and phosphorus absorption of mulberry as well. Additionally, the mixed suspensions of PGPF induced the production of catalase, soluble sugar, and chlorophyll, which in turn enhanced the drought tolerance of mulberry and accelerated their growth recovery after drought. Collectively, these findings might provide new insights into improving mulberry drought tolerance and further boosting mulberry fruit yields by exploiting interactions between hosts and PGPF.
Novel Silver Complexes Based on Phosphanes and Ester Derivatives of Bis(pyrazol-1-yl)acetate Ligands Targeting TrxR: New Promising Chemotherapeutic Tools Relevant to SCLC Management
Authors: Pellei Maura , Santini Carlo , Bagnarelli Luca , Caviglia Miriam , Sgarbossa Paolo , De Franco Michele , Zancato Mirella , Marzano Cristina , Gandin Valentina
Published: February 2023

Bis(pyrazol-1-yl)acetic acid (HC(pz)2COOH) and bis(3,5-dimethyl-pyrazol-1-yl)acetic acid (HC(pzMe2)2COOH) were converted into the methyl ester derivatives 1 (LOMe) and 2 (L2OMe), respectively, and were used for the preparation of silver(I) complexes 3–5. The Ag(I) complexes were prepared by the reaction of AgNO3 and 1,3,5-triaza-7-phosphaadamantane (PTA) or triphenylphosphine (PPh3) with LOMe and L2OMe in methanol solution. All Ag(I) complexes showed a significant in vitro antitumor activity, proving to be more effective than the reference drug cisplatin in the in-house human cancer cell line panel containing examples of different solid tumors. Compounds were particularly effective against the highly aggressive and intrinsically resistant human small-cell lung carcinoma (SCLC) cells, either in 2D and 3D cancer cell models. Mechanistic studies revealed their ability to accumulate into cancer cells and to selectively target Thioredoxin (TrxR), thus leading to redox homeostasis unbalance and ultimately inducing cancer cell death through apoptosis.
Molecular Responses of Daphnids to Chronic Exposures to Pharmaceuticals
Authors: O’Rourke Katie , Engelmann Beatrice , Altenburger Rolf , Rolle-Kampczyk Ulrike , Grintzalis Konstantinos
Published: February 2023

Pharmaceutical compounds are among several classes of contaminants of emerging concern, such as pesticides, heavy metals and personal care products, all of which are a major concern for aquatic ecosystems. The hazards posed by the presence of pharmaceutical is one which affects both freshwater organisms and human health—via non-target effects and by the contamination of drinking water sources. The molecular and phenotypic alterations of five pharmaceuticals which are commonly present in the aquatic environment were explored in daphnids under chronic exposures. Markers of physiology such as enzyme activities were combined with metabolic perturbations to assess the impact of metformin, diclofenac, gabapentin, carbamazepine and gemfibrozil on daphnids. Enzyme activity of markers of physiology included phosphatases, lipase, peptidase, β-galactosidase, lactate dehydrogenase, glutathione-S-transferase and glutathione reductase activities. Furthermore, targeted LC-MS/MS analysis focusing on glycolysis, the pentose phosphate pathway and the TCA cycle intermediates was performed to assess metabolic alterations. Exposure to pharmaceuticals resulted in the changes in activity for several enzymes of metabolism and the detoxification enzyme glutathione-S-transferase. Metabolic perturbations on key pathways revealed distinct groups and metabolic fingerprints for the different exposures and their mixtures. Chronic exposure to pharmaceuticals at low concentrations revealed significant alterations of metabolic and physiological endpoints.
Tissue-Oxygen-Adaptation of Bone Marrow-Derived Mesenchymal Stromal Cells Enhances Their Immunomodulatory and Pro-Angiogenic Capacity, Resulting in Accelerated Healing of Chemical Burns
Authors: Volkova Marina V. , Shen Ningfei , Polyanskaya Anna , Qi Xiaoli , Boyarintsev Valery V. , Kovaleva Elena V. , Trofimenko Alexander V. , Filkov Gleb I. , Mezentsev Alexandre V. , Rybalkin Sergey P. , Durymanov Mikhail O.
Published: February 2023

Transplantation of mesenchymal stromal cells (MSCs) provides a powerful tool for the management of multiple tissue injuries. However, poor survival of exogenous cells at the site of injury is a major complication that impairs MSC therapeutic efficacy. It has been found that tissue-oxygen adaptation or hypoxic pre-conditioning of MSCs could improve the healing process. Here, we investigated the effect of low oxygen tension on the regenerative potential of bone-marrow MSCs. It turned out that incubation of MSCs under a 5% oxygen atmosphere resulted in increased proliferative activity and enhanced expression of multiple cytokines and growth factors. Conditioned growth medium from low-oxygen-adapted MSCs modulated the pro-inflammatory activity of LPS-activated macrophages and stimulated tube formation by endotheliocytes to a much higher extent than conditioned medium from MSCs cultured in a 21% oxygen atmosphere. Moreover, we examined the regenerative potential of tissue-oxygen-adapted and normoxic MSCs in an alkali-burn injury model on mice. It has been revealed that tissue-oxygen adaptation of MSCs accelerated wound re-epithelialization and improved the tissue histology of the healed wounds in comparison with normoxic MSC-treated and non-treated wounds. Overall, this study suggests that MSC adaptation to ‘physiological hypoxia’ could be a promising approach for facilitating skin injuries, including chemical burns.
Using Zebrafish Animal Model to Study the Genetic Underpinning and Mechanism of Arrhythmogenic Cardiomyopathy
Authors: Niu Yujuan , Sun Yuanchao , Liu Yuting , Du Ke , Xu Xiaolei , Ding Yonghe
Published: February 2023

Arrhythmogenic cardiomyopathy (ACM) is largely an autosomal dominant genetic disorder manifesting fibrofatty infiltration and ventricular arrhythmia with predominantly right ventricular involvement. ACM is one of the major conditions associated with an increased risk of sudden cardiac death, most notably in young individuals and athletes. ACM has strong genetic determinants, and genetic variants in more than 25 genes have been identified to be associated with ACM, accounting for approximately 60% of ACM cases. Genetic studies of ACM in vertebrate animal models such as zebrafish (Danio rerio), which are highly amenable to large-scale genetic and drug screenings, offer unique opportunities to identify and functionally assess new genetic variants associated with ACM and to dissect the underlying molecular and cellular mechanisms at the whole-organism level. Here, we summarize key genes implicated in ACM. We discuss the use of zebrafish models, categorized according to gene manipulation approaches, such as gene knockdown, gene knock-out, transgenic overexpression, and CRISPR/Cas9-mediated knock-in, to study the genetic underpinning and mechanism of ACM. Information gained from genetic and pharmacogenomic studies in such animal models can not only increase our understanding of the pathophysiology of disease progression, but also guide disease diagnosis, prognosis, and the development of innovative therapeutic strategies.
Development of Specific Monoclonal Antibodies against Porcine RIG-I-like Receptors Revealed the Species Specificity
Authors: Shao Qi , Li Shuangjie , Cao Qi , Gu Haotian , Zhang Jiajia , Zhang Youwen , Zhang Kaili , Zheng Wanglong , Chen Nanhua , Shang Shaobin , Zhu Jianzhong
Published: February 2023

The RIG-I-like receptors (RLRs) play critical roles in sensing and combating viral infections, particularly RNA virus infections. However, there is a dearth of research on livestock RLRs due to a lack of specific antibodies. In this study, we purified porcine RLR proteins and developed monoclonal antibodies (mAbs) against porcine RLR members RIG-I, MDA5 and LGP2, for which one, one and two hybridomas were obtained, respectively. The porcine RIG-I and MDA5 mAbs each targeted the regions beyond the N-terminal CARDs domains, whereas the two LGP2 mAbs were both directed to the N-terminal helicase ATP binding domain in the Western blotting. In addition, all of the porcine RLR mAbs recognized the corresponding cytoplasmic RLR proteins in the immunofluorescence and immunochemistry assays. Importantly, both RIG-I and MDA5 mAbs are porcine specific, without demonstrating any cross-reactions with the human counterparts. As for the two LGP2 mAbs, one is porcine specific, whereas another one reacts with both porcine and human LGP2. Thus, our study not only provides useful tools for porcine RLR antiviral signaling research, but also reveals the porcine species specificity, giving significant insights into porcine innate immunity and immune biology.
Unraveling the Impact of Intratumoral Heterogeneity on EGFR Tyrosine Kinase Inhibitor Resistance in EGFR-Mutated NSCLC
Authors: Kobayashi Keigo , Tan Aaron C.
Published: February 2023

The advent of tyrosine kinase inhibitors (TKIs) for treating epidermal growth factor receptor (EGFR)-mutated non-small-cell lung cancer (NSCLC) has been a game changer in lung cancer therapy. However, patients often develop resistance to the drugs within a few years. Despite numerous studies that have explored resistance mechanisms, particularly in regards to collateral signal pathway activation, the underlying biology of resistance remains largely unknown. This review focuses on the resistance mechanisms of EGFR-mutated NSCLC from the standpoint of intratumoral heterogeneity, as the biological mechanisms behind resistance are diverse and largely unclear. There exist various subclonal tumor populations in an individual tumor. For lung cancer patients, drug-tolerant persister (DTP) cell populations may have a pivotal role in accelerating the evolution of tumor resistance to treatment through neutral selection. Cancer cells undergo various changes to adapt to the new tumor microenvironment caused by drug exposure. DTP cells may play a crucial role in this adaptation and may be fundamental in mechanisms of resistance. Intratumoral heterogeneity may also be precipitated by DNA gains and losses through chromosomal instability, and the role of extrachromosomal DNA (ecDNA) may play an important role. Significantly, ecDNA can increase oncogene copy number alterations and enhance intratumoral heterogeneity more effectively than chromosomal instability. Additionally, advances in comprehensive genomic profiling have given us insights into various mutations and concurrent genetic alterations other than EGFR mutations, inducing primary resistance in the context of tumor heterogeneity. Understanding the mechanisms of resistance is clinically crucial since these molecular interlayers in cancer-resistance mechanisms may help to devise novel and individualized anticancer therapeutic approaches.
Recent Insights into the Functional Role of AMPA Receptors in the Oligodendrocyte Lineage Cells In Vivo
Authors: Kukley Maria
Published: February 2023

This review discusses the experimental findings of several recent studies which investigated the functional role of AMPA receptors (AMPARs) in oligodendrocyte lineage cells in vivo, in mice and in zebrafish. These studies provided valuable information showing that oligodendroglial AMPARs may be involved in the modulation of proliferation, differentiation, and migration of oligodendroglial progenitors, as well as survival of myelinating oligodendrocytes during physiological conditions in vivo. They also suggested that targeting the subunit composition of AMPARs may be an important strategy for treating diseases. However, at the same time, the experimental findings taken together still do not provide a clear picture on the topic. Hence, new ideas and new experimental designs are required for understanding the functional role of AMPARs in the oligodendrocyte lineage cells in vivo. It is also necessary to consider more closely the temporal and spatial aspects of AMPAR-mediated signalling in the oligodendrocyte lineage cells. These two important aspects are routinely discussed by neuronal physiologists studying glutamatergic synaptic transmission, but are rarely debated and thought about by researchers studying glial cells.
Structure–Activity Relationship Analysis of Rhosin, a RhoA GTPase Inhibitor, Reveals a New Class of Antiplatelet Agents
Authors: Dandamudi Akhila , Seibel William , Tourdot Benjamin , Cancelas Jose A. , Akbar Huzoor , Zheng Yi
Published: February 2023

Current antiplatelet therapies have several clinical complications and are mostly irreversible in terms of suppressing platelet activity; hence, there is a need to develop improved therapeutic agents. Previous studies have implicated RhoA in platelet activation. Here, we further characterized the lead RhoA inhibitor, Rhosin/G04, in platelet function and present structure–activity relationship (SAR) analysis. A screening for Rhosin/G04 analogs in our chemical library by similarity and substructure searches revealed compounds that showed enhanced antiplatelet activity and suppressed RhoA activity and signaling. A screening for Rhosin/G04 analogs in our chemical library using similarity and substructure searches revealed compounds that showed enhanced antiplatelet activity and suppressed RhoA activity and signaling. SAR analysis revealed that the active compounds have a quinoline group optimally attached to the hydrazine at the 4-position and halogen substituents at the 7- or 8-position. Having indole, methylphenyl, or dichloro-phenyl substituents led to better potency. Rhosin/G04 contains a pair of enantiomers, and S-G04 is significantly more potent than R-G04 in inhibiting RhoA activation and platelet aggregation. Furthermore, the inhibitory effect is reversible, and S-G04 is capable of inhibiting diverse-agonist-stimulated platelet activation. This study identified a new generation of small-molecule RhoA inhibitors, including an enantiomer capable of broadly and reversibly modulating platelet activity.