bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Polymorphic Index Model for Assessing Subtropical Secondary Natural Oak Forest Site Quality Under Complex Site and Climate Variables
Authors: Huang Lang , Zhu Guangyu , Chen Guoqi
Published: October 2024

Site and climate conditions are the key determinants controlling dominant height growth and forest productivity, both independently and interactively. Secondary natural oak forests are a typical forest type in China, especially in Hunan Province, but little is known about the site index of this forest under the complex site and climate variables in the subtropics. Based on survey data of dominant trees and site variables from 101 plots in Hunan oak natural secondary forests and climate data obtained using spatial interpolation, we used the random forest method, correlation analysis, and the analysis of variance to determine the main site and climate factors affecting oak forest dominant height and proposed a modeling method of an oak natural secondary forest site index based on the random effect of site–climate interaction type. Of the site variables, elevation affected stand dominant height the most, followed by slope direction and position. Winter precipitation and summer mean maximum temperature had the greatest impact on stand dominant height. To develop the modeling method, we created 10 popular base models but found low performance (R2 ranged from 0.1731 to 0.2030). The optimal base model was Mitscherlich form M3 (R2 = 0.1940) based on parameter significance tests. Since site and climate factors affect the site index curve, the dominant site and climate factors were combined into site types and climate types, respectively, and a nonlinear mixed-effects approach was used to simulate different site types, climate types, site–climate interaction types, and their combinations as random effects. Site–climate interaction type as a random factor enhanced model (M3.4) performance and prediction accuracy (R2 from 0.1940 to 0.8220) compared to the optimum base model. After clustering the 62 site–climate interaction types into three, five, and eight groups using hierarchical clustering, a mixed-effects model with the random effects of eight groups improved model performance (R2 = 0.8265) and applicability. The modeling method developed in this study could be used to assess a regional secondary natural oak forest site index under complex site and climate variables to evaluate the forest productivity.
Integrating Forest Ecosystem Services into Health Strategies to Improve Human Well-Being
Authors: Liu Yuan , Wang Chengyuan , Liu Yuwen , Feng Tiantian , Wang Enheng , Yang Liwei , Niu Qian , Mao Xuegang
Published: October 2024

As the largest terrestrial ecosystem covering extensive expanses of the Earth’s surface, forests offer crucial health benefits to humans, both directly and indirectly. Presently, health services derived from forest resources have presented significant opportunities for enhancing human well-being. Nonetheless, the absence of a comprehensive understanding regarding the mechanisms by which forests impact human health jeopardizes the potential gains in health. Regrettably, there remains a dearth of scholarly work elucidating these pathways. This paper aims to furnish a thorough examination of how forests influence human health. We initiate by formulating a conceptual framework upon which we delineate the various pathways through which forests impact human health. These encompass the provisioning of resources, preventive services, and forest therapies. Concurrently, we outline the moderating influence of social, economic, and individual characteristics as mediators within this pathway. These characteristics are classified into two overarching dimensions: accessibility and behavioral choices, which notably affect marginalized demographics such as those with lower socioeconomic status, women, the elderly, individuals with disabilities, and children in developing nations. Consequently, we build upon these foundational insights to propose six strategies aimed at perpetuating the positive impact of forests on human health in the foreseeable future. In the future, the development of forest management policies, the assessment of long-term health benefits, social practices, and international cooperation must be considered holistically to attain the dual objective of sustainable forest management and the advancement of human well-being.
Development and Application of Microsatellite Markers for Genetic Diversity Assessment and Construction of a Core Collection of Myrciaria dubia (Kunth) McVaugh Germplasm from the Peruvian Amazon
Authors: Castro Juan C. , Vasquez-Guizado Stalin J. , Vigil Bianca E. , Ascue Francisco , Rojas-Villa Naysha , Paredes Jae D. , Cobos Marianela , Castro Carlos G. , Motta Daniel E. , Adrianzén Pedro M. , Imán Sixto A. , Maddox J. Dylan
Published: October 2024

The Amazonian shrub Myrciaria dubia (camu-camu) produces vitamin C-rich fruits of growing commercial interest. However, sustainable utilization requires assessment and protection of the genetic diversity of the available germplasm. This study aimed to develop and apply microsatellite markers to assess genetic diversity and construct a core collection of M. dubia germplasm from the Peruvian Amazon. Sixteen polymorphic microsatellite loci were developed using an enrichment approach. The evaluation of 336 genotypes from 43 accessions of the germplasm bank, originating from eight river basins, was conducted using these newly developed markers. Genetic diversity parameters, including observed and expected heterozygosity, were calculated. Analysis of molecular variance (AMOVA) was performed to assess the distribution of genetic variation within and among accessions and river basins. Bayesian clustering analysis was employed to infer population structure. A core collection was constructed to maximize allelic richness. High genetic diversity was observed, with heterozygosity values ranging from 0.468 to 0.644 (observed) and 0.684 to 0.817 (expected) at the river basin level. AMOVA indicated significant genetic variation within (73–86%) compared to among (14–27%) accessions and river basins. Bayesian clustering detected ten genetic clusters, with several degrees of admixture among river basins, except for the genetically homogeneous Putumayo River basin. A core collection comprising 84 plant genotypes (25% of the full collection) was established, representing 90.82% of the overall allelic diversity. These results have important implications for M. dubia conservation strategies and breeding programs, in demonstrating a need for genetic connectivity between populations but preserving unique genetic resources in isolated basins. These results validate the expected levels of diversity and population subdivision in a crop and stress the need to secure genetically diverse germplasms, underscoring the importance of thorough genetic characterization for ex situ germplasm management.
Deriving Vegetation Indices for 3D Canopy Chlorophyll Content Mapping Using Radiative Transfer Modelling
Authors: Elsherif Ahmed , Smigaj Magdalena , Gaulton Rachel , Gastellu-Etchegorry Jean-Philippe , Shenkin Alexander
Published: October 2024

Leaf chlorophyll content is a major indicator of plant health and productivity. Optical remote sensing estimation of chlorophyll limits its retrievals to two-dimensional (2D) estimates, not allowing examination of its distribution within the canopy, although it exhibits large variation across the vertical profile. Multispectral and hyperspectral Terrestrial Laser Scanning (TLS) instruments can produce three-dimensional (3D) chlorophyll estimates but are not widely available. Thus, in this study, 14 chlorophyll vegetation indices were developed using six wavelengths employed in commercial TLS instruments (532 nm, 670 nm, 808 nm, 785 nm, 1064 nm, and 1550 nm). For this, 200 simulations were carried out using the novel bidirectional mode in the Discrete Anisotropic Radiative Transfer (DART) model and a realistic forest stand. The results showed that the Green Normalized Difference Vegetation Index (GNDVI) of the 532 nm and either the 808 nm or the 785 nm wavelengths were highly correlated to the chlorophyll content (R2 = 0.74). The Chlorophyll Index (CI) and Green Simple Ratio (GSR) of the same wavelengths also displayed good correlation (R2 = 0.73). This study was a step towards canopy 3D chlorophyll retrieval using commercial TLS instruments, but methods to couple the data from the different instruments still need to be developed.
Unraveling the Biosynthetic Logic Behind the Production of Ramoplanin and Related Lipodepsipeptide Antibiotics
Authors: Yushchuk Oleksandr , Zhukrovska Kseniia , Binda Elisa , Marinelli Flavia
Published: October 2024

This review focuses on the genetic and biotechnological aspects of the biosynthesis of ramoplanin (Rmp), enduracidin (End), and other related lipodepsipeptide antibiotics, herein named collectively ramoplanin and ramoplanin-related lipodepsipeptide (RRLDPs). These compounds exhibit a promising antimicrobial activity against Gram-positive bacterial pathogens, showing no cross-resistance with vancomycin. Rmp is in clinical development for human treatment and End has been used as animal growth promoter for decades. Other RRLDPs as ramoplanose and janiemycin had been poorly investigated in the past, whereas new molecules as chersinamycin have been recently discovered, attracting a renewed interest in this class of antibiotics. Nowadays, sequence and annotation of the biosynthetic gene clusters (BGCs) of Rmp, End, and several other RRLDPs are available, and researchers are focused on understanding the biosynthetic logic behind the production of these compounds. Interestingly, producers of Rmp and chersinamycin belong to the so-called “non-common” actinomycetes from the family Micromonosporaceae, whereas End is produced by different members of the genus Streptomyces. To the best of our knowledge, no reviews summarize and systematize the current information on the biosynthesis of RRLDPs. Therefore, in this review, we aim to fill this gap. We first describe and compare the BGCs for known RRLDPs, giving an insight on how they were discovered and developed. Next, we review the biosynthetic pathways of these antibiotics, as well as the regulation of their biosynthesis. Then, we focus on the production processes of RRLDPs, demonstrating how cultivation and nutritional factors influence their production. Finally, we provide a short outline of future directions in studying RRLDPs.
Age, Growth, Sex Composition, and Diet of the Burbot, Lota lota, the Only Freshwater Species of the Family Lotidae in the Amur (Heilongjiang) River, Northeast China
Authors: Li Lei , Shao Huili , Mikheev Pavel B. , Zhang Zepeng , Jin Hongyu , Lu Wanqiao
Published: October 2024

Information about the population structure, including the age and sex composition, growth characteristics, and diet of fish, is essential for the conservation and sustainable exploitation of fish stock. The burbot, Lota lota, is the only freshwater species of the family Lotidae in the Amur (Heilongjiang) River catchment located in northeastern China. Information on the biological characteristics and data on the population structure of this fish from the Amur River are scarce. To study these factors in burbot, Lota lota, in the Amur River of China, 749 specimens from four sampling areas were taken and analyzed in October 2022, January 2023, and May 2023. The ages of the sampled fish ranged from 1+ to 7+ years. The female/male ratio was 1.04:1, and body length and mass varied from 175 to 595 mm and 73.5 to 1958.7 g, respectively. The length–weight regression parameter b value was estimated as 2.80. The parameters of the von Bertalanffy growth model were L∞ = 596, K = 0.221, and t0 = −0.771 for all sampled fish; L∞ = 625, K = 0.208, and t0 = −0.756 for females; and L∞ = 584, K = 0.219, and t0 = −0.980 for males. The analysis of the stomach contents showed fish to be the major source of nutrition across all areas and sampling periods. In January, the secondary prey of burbots in the Huma reach of the upper Amur River was aquatic insect larvae, while, in the Tongjiang and Fuyuan reaches of the middle Amur River, the secondary prey was shrimp. The mean stomach fullness index and rate of empty stomachs differed with the sampling area, with the greatest proportion of empty stomachs observed in the Luobei reach of the middle Amur River, and the lowest in the Tongjiang and Fuyuan reaches. The mean stomach fullness index showed the opposite trend. In the Huma reach, the contributions of fish to the diet and the mean stomach fullness index were significantly higher in May than in October and January. The study provides the first detailed information on the population age and sex structure, growth patterns, and feeding ecology of burbots from the Amur River, China. The results will aid in formulating management strategies and regulations for local populations of burbots in the Amur River, China.
Consumers’ Attitudes Towards Prawn Consumption in Bangladesh: An Investigation on Perceived Value and Willingness-to-Pay
Authors: Uddin Md Taj , Roy Sima , Dhar Aurup Ratan
Published: October 2024

This study assessed consumer preferences and consumption patterns for prawns in Bangladesh, underscoring the economic and nutritional significance of prawns in the local diet. A total of 120 respondents were interviewed following a random sampling technique from four districts. The data analysis employed descriptive statistics alongside mathematical and statistical methods. The findings indicate a strong preference for fresh and large-sized prawns among the consumers. Income level substantially influenced the prawn purchases, with the high-income consumers more likely to buy premium prawn products, while the low-income consumers showed sensitivity to price changes. The primary concerns that significantly influenced the consumers’ purchasing decisions were found to be size, freshness, taste, price, market availability, and income. Additionally, the consumers’ perceived value and willingness-to-pay for prawns were significantly driven by size, freshness, environmental sustainability, and income levels. Policy recommendations include promoting sustainable prawn farming and improving accessibility for low-income consumers to enhance both nutritional intake and economic resilience. This research offers valuable insights for stakeholders in the prawn industry, facilitating the development of targeted marketing strategies and informed policymaking for sustainable industry growth.
Occurrence of Multi-Drug-Resistant Bacteria in Rainbow Trout–Lettuce Bio-Integrated Culture Systems in Chile
Authors: Concha Christopher , Hurtado Luz , Pérez Norma , Merino German E. , Miranda Claudio D.
Published: October 2024

In an aquaponic system, fish and plants are cultivated together in a symbiotic environment where they mutually benefit, using significantly less water than traditional farming methods. The main aim of this study was to investigate the occurrence of antimicrobial resistance in two aquaponic systems implemented in two Chilean high schools using rainbow trout and lettuce cultures. When water samples (fish tank, biofilter, and plant raft) were analyzed over a three-month period, no resistance to oxytetracycline was detected, whereas the occurrence of resistance to florfenicol was rather small, ranging from 0.01% to 3.1% of bacterial culturable counts. Eighteen isolates were recovered from various sources as representatives of the florfenicol-resistant population, and all of them belonged to the Pseudomonas genus, showing a multi-drug-resistance phenotype and exhibiting simultaneous resistance to 7–13 antimicrobials. All isolates exhibited resistance to amoxicillin, chloramphenicol, florfenicol, and furazolidone and susceptibility to meropenem, oxytetracycline, oxolinic acid, flumequine, ciprofloxacin, and enrofloxacin. Five and two isolates carried the amphenicol-resistance-encoding genes floR and cmlA, respectively, whereas no carriage of integrons or the fexA, fexB, pexA, optrA, and cfr genes encoding for florfenicol resistance was detected. Eleven isolates carried plasmids, but only two of them were able to transfer their plasmid content by conjugation. The knowledge of the microbiome associated with aquaponic systems is still scarce, and their role as potential reservoirs of antimicrobial-resistant bacteria and related genes of these systems must be elucidated.
The Effect of Cold Plasma Treatment on the Storage Stability of Mushrooms (Agaricus bisporus)
Authors: Guo Yalong , Xia Shuqiong , Shi Chong , Ma Ning , Pei Fei , Yang Wenjian , Hu Qiuhui , Kimatu Benard Muinde , Fang Donglu
Published: October 2024

Postharvest Agaricus bisporus is susceptible to browning, water loss, and microbial infection. In order to extend its shelf life, cold plasma technology was used to treat and evaluate A. bisporus. Firstly, according to the results of a single factor test and response surface analysis, the optimal conditions for cold plasma treatment were determined as a voltage of 95 kV, a frequency of 130 Hz, and a processing time of 10 min. Secondly, storage experiments were carried out using the optimized cold plasma treatment. The results showed that the cold plasma treatment in the packaging significantly reduced the total viable count in A. bisporus by approximately 16.5%, maintained a browning degree at 26.9% lower than that of the control group, and a hardness at 25.6% higher than that of the control group. In addition, the cold plasma treatment also helped to preserve the vitamin C and total protein content of A. bisporus. In conclusion, cold plasma treatment showed great potential in enhancing the postharvest quality of fresh A. bisporus.
Microstructural Modification and Sorption Capacity of Green Coffee Beans
Authors: Dong Weixue , Kitamura Yutaka , Kokawa Mito , Suzuki Taroh , Amini Rasool Khan
Published: October 2024

To enhance the pore structure of green coffee beans (GCB) and detect the sorption capacity and extraction characteristics of flavor compounds before roasting, this study employed several methods: hot air drying (HD), freeze-drying (FD), 3-levels short-time heating with puffing (SH1P, SH2P, and SH3P), and 3-levels microwave with puffing (MW45P, MW60P, and MW75P). These methods were applied to GCBs pre-soaked in water for different times. The effects of these treatments on color change, porosity, microstructure, citric acid sorption capacity, and caffeine and chlorogenic acid extraction yield were investigated. Results indicated that, except for GCBs treated with SH1P, SH2P, SH3P, and MW75P, all other modified GCBs showed minimal color change. GCBs treated with MW60P exhibited favorable pore structures. MW60P treatments significantly improved the extraction yield of caffeine and chlorogenic acid. Furthermore, the increased porosity and improved pore size distribution of GCB after MW60P resulted in a significant increase in the sorption of citric acid onto modified GCB. The rate of the sorption reaction followed the pseudo-first-order kinetics. In conclusion, MW60P are effective treatments for enhancing the microstructure of GCB, improving sorption capacity, and improving the extraction yield of flavor compounds.
A Multi-Isotopic Chemometric Approach for Tracing Hazelnut Origins
Authors: Torres-Cobos Berta , Rosell Mònica , Soler Albert , Rovira Mercè , Romero Agustí , Guardiola Francesc , Vichi Stefania , Tres Alba
Published: October 2024

High-value products, such as hazelnuts, are particularly vulnerable to fraud due to their price dependence on geographical origin. Guaranteeing hazelnuts’ authenticity is essential for consumer trust and safety. Stable isotope analysis has become a reference method for origin authentication as it is reliable, robust, and easily transferable across laboratories. However, multiple isotopic markers coupled with chemometric techniques are often needed to authenticate food provenance accurately. In this study, we focused on assessing the potential of bulk δ18O, along with δ2H and δ13C of the main fatty acids, as hazelnut-origin authenticity markers. PLS-DA classification models were developed to differentiate samples (n = 207) according to their region of origin. This multi-isotopic approach provided promising external validation results, achieving a 94% global correct classification rate in discriminating hazelnuts from regions with distinct geographical and environmental conditions. This study lays the groundwork for further model development and evaluation across additional production areas and harvest years.
Comparative Study on Protein Composition and Foam Characteristics of Barley and Wheat Beer
Authors: Li Xiu , Jiang Kai , Jin Yuhong , Liu Junhan
Published: October 2024

Protein is an important component of beer, and its type, content and molecular weight directly affect the quality of beer, especially the foam quality of beer. Different brands of wheat beer and barley beer available in the market were used for this analysis. The differences in protein composition and foam performance between multi-sample barley and wheat beer were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-pressure size exclusion chromatography. Protein significantly influences beer quality, particularly its foam properties. Wheat beer (WB) has 9.52–84.10% more total protein content than barley beer (BB). The primary proteins in both beers are 6.9–20.1 kDa, with WB having 1.04 g/L more of this protein, 60.11% higher than that of BB. It is one of the main different proteins between WB and BB. WB also contains 66.67% more 20.1–32.4 kDa protein compared to BB. This is one of the main differences between WB and BB proteins. Both 6.9–20.1 kDa and 20.1–32.4 kDa proteins enhance beer viscosity and foam properties. Additionally, WB’s > 32.4 kDa protein content is 246.67% higher than BB’s, significantly improving beer hydrophobicity and foam performance. These protein differences are key factors in the superior foam quality of WB.
Improvement of Storage Quality of Broccoli Using a Cold-Shock Precooling Way and the Related Molecular Mechanisms
Authors: Guo Xiaoqian , Liu Weihua , Zhang Liyong , Zhu Xinyue , Wang Xianghong , Mi Si
Published: October 2024

This research was performed to ascertain the impact of cold shock precooling and the underlying mechanism on broccoli storage quality. After being harvested and placed at 0 ± 2 °C, the broccoli was sealed in polyethylene bags and stored at 4 ± 2 °C. Cold-shock precooling showed superior qualities in terms of higher hardness, titratable acidity, moisture content, soluble protein, and chlorophyll, as well as more abundant volatile compounds, better sensory quality, antioxidant capacity, and decreased weight loss in comparison to without cold shock. The regulation of important metabolic enzymes such as peroxidase, catalase, pheophytinase, and magnesium-dechelatase was credited with these beneficial effects. It was found that a 90 min duration of cold shock was the ideal treatment. Results showed that cold shock precooling was a useful, economical, and environmentally responsible way to reduce postharvest loss and postpone broccoli senescence during storage.
Inhibition of Food Spoilage Fungi, Botrytis cinerea and Rhizopus sp., by Nanoparticles Loaded with Baccharis dracunculifolia Essential Oil and Nerolidol
Authors: Corrêa Aldrey Nathália Ribeiro , Clerici Naiara Jacinta , de Paula Natália Oliveira , Brandelli Adriano
Published: October 2024

This study investigates the antifungal potential of encapsulated essential oil (EO) from Baccharis dracunculifolia and nerolidol (NE) within Pluronic® F-127 nanoparticles (NPs). The EO, containing nerolidol, β-caryophyllene, and α-pinene as major bioactive compounds, exhibited superior antifungal activity compared to NE. The NP-EO formulations demonstrated high efficacy against Botrytis cinerea, with inhibition rates ranging from 29.73% to 87.60% and moderate efficacy against Rhizopus sp., with inhibition rates from 11.81% to 32.73%. In comparison, NP-NE showed lower antifungal activity. Both formulations effectively inhibited spore germination, with NP-EO showing greater inhibition compared to NP-NE. The encapsulation efficiency was significantly higher for NP-EO (80.1%) as compared to NP-NE (51.1%), attributed to the complex composition of EO facilitating better encapsulation and retention. Stability studies indicated that both NP formulations were stable at 25 °C for at least 15 days and exhibited changes in particle size and the formation of smaller particle populations at other temperatures (4 °C and 37 °C). Hemolytic activity was low across all NPs, suggesting their safety for food applications. The findings underscore the efficacy and applicability of EO-encapsulated NPs in extending food shelf life and maintaining product quality. The controlled and prolonged release of active compounds, coupled with their antifungal activity and safety, suggests that these NPs represent a promising and innovative approach for food preservation and active packaging development.
Development of Oil Industry in Poland in the Context of the European Union
Authors: Bełdycka-Bórawska Aneta , Bórawski Piotr , Holden Lisa , Rokicki Tomasz
Published: October 2024

Oil industry is developing well in Poland. This study aimed to examine the development of the Polish oil industry after joining the European Union. The following questions were asked: What were the changes in the consumption of vegetable oils in Poland after EU enlargement in 2004? How did the production of vegetable oil change in Poland after EU enlargement in 2004? And has the liquidity of the Polish oil industry changed after EU enlargement in 2004? First, the changes in the oil industry were evaluated. This research proved the development of the fat industry. We used the regression model to analyze the impact of chosen factors on the economic situation of the oil industry in Poland. Rapeseed is the main source of oil for the edible oil and biodiesel industry. High prices of rapeseed increased the sown area of rapeseed. The production of crude rapeseed oil changed from 520.1 thousand tons in 2005 to 1440 thousand tons in 2023. Biofuels made from rapeseed oils are called first generation. Poland’s production of refined rapeseed oil changed from 386.5 thousand tons in 2005 to 541 thousand tons in 2023. These numbers confirm the development of edible oil and biofuel production. The gross profit increased from PLN 133 mln in 2005 to PLN 443.8 mln in 2023. The net profit of the fat industry increased from PLN 110 mln in 2005 to PLN 381.6 mln in 2023. The oil industry is characterized by high investment, which reached the level of PLN 232.9 mln in 2023. The investment rate in 2023 was PLN 1.96 %. The changes observed in the rapeseed market after Poland joined the EU affected the performance of vegetable oil and biofuel producers. The regression analysis proved the hypothesis that the monthly consumption of rapeseed oil is the main factor influencing the oil industry situation in Poland. The oil industry is important to the development of the whole economy. Particular attention should be paid to current liquidity, which is why a constant supply of money from sold oil and biodiesel is needed to cover costs.
The 17O/18O Ratio of Post-AGB Sources: Canonical and Non-Canonical Populations
Authors: Alcolea Javier , Masa Elisa , Khouri Theo , Santander-García Miguel , Gallardo Cava Iván , Olofsson Hans , Sánchez Contreras Carmen , Bujarrabal Valentín , Vlemmings Wouter H. T. , Tafoya Daniel
Published: October 2024

Stellar evolution models serve as tools to derive stellar parameters from elemental and isotopic abundance ratios. For low-to-intermediate mass evolved stars, C/O, 12C/13C, and 17O/18O ratios are proxies of the initial mass, a largely unknown parameter in post-AGB sources, yet fundamental to establish correlations with the main properties of their post-AGB envelopes, progressing in understanding their formation and evolution. In these sources, the C/O ratio can be constrained from the detection of C- or O-bearing species in addition to CO, while the 17O/18O ratio is straightforwardly determined from the C17O-to-C18O intensity ratio of rotational lines. However, the theory is at odds with the observations. We review the status of the question, including new accurate 17O/18O ratios for 11 targets (totaling 29). Comparing the results for the 17O/18O ratios and C-rich/O-rich chemical composition, we find that ∼45% of the cases are canonical, i.e., the observations align with standard model predictions. O-rich non-canonical sources, with 17O/18O ratios above the expected, can be explained by a premature interruption of their AGB evolution as a consequence of a quasi-explosive ejection of a large fraction of the initial mass. For non-canonical C-rich sources, with 17O/18O ratios below predictions, we suggest the possibility they are extrinsic C-rich stars.
Smart Nanocomposite Hydrogels as Next-Generation Therapeutic and Diagnostic Solutions
Authors: Valentino Anna , Yazdanpanah Sorur , Conte Raffaele , Calarco Anna , Peluso Gianfranco
Published: October 2024

Stimuli-responsive nanocomposite gels combine the unique properties of hydrogels with those of nanoparticles, thus avoiding the suboptimal results of single components and creating versatile, multi-functional platforms for therapeutic and diagnostic applications. These hybrid materials are engineered to respond to various internal and external stimuli, such as temperature, pH, light, magnetic fields, and enzymatic activity, allowing precise control over drug release, tissue regeneration, and biosensing. Their responsiveness to environmental cues permits personalized medicine approaches, providing dynamic control over therapeutic interventions and real-time diagnostic capabilities. This review explores recent advances in stimuli-responsive hybrid gels’ synthesis and application, including drug delivery, tissue engineering, and diagnostics. Overall, these platforms have significant clinical potential, and future research is expected to lead to unique solutions to address unmet medical needs.
Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment
Authors: Woo Chaewon , Byun Jina , Shin Sung Gyu , Yoo Heeseon , Cho Sungwoo , Lee Donghun , Park Taezoon , Jeong Jae Hyun
Published: October 2024

We developed a tailored 3D Agarose-well system integrated with reconstructed human skin equivalents to enhance skin penetration assessments. This system addresses common limitations in traditional trans-well reconstructions, such as dermal layer contraction and limited lateral diffusion, by entangling collagen fibrils within the Agarose-well. We evaluated the penetration behavior of three peptides, with and without skin-penetrating peptide (SPP) sequences, alongside adenosine, a known anti-wrinkle agent. Despite a SPP having a molecular weight approximately four times greater than that of adenosine, its kinetic constant was similar, with values of about 39 and 34, respectively. Moreover, this living skin equivalent system not only allowed for the evaluation of adenosine penetration, but also demonstrated its biological effects, with adenosine significantly enhancing procollagen synthesis by approximately 23% compared to the control. Overall, this novel strategy holds the potential for tailoring 3D Agarose-wells and advancing high-performance gel development, making it a promising approach for applications in tissue engineering, medical science, regenerative medicine, and cosmetics.
Reproducible Superinsulation Materials: Organosilica-Based Hybrid Aerogels with Flexibility Control
Authors: Geyer Marvin , Leven Felix , Limberg Johannes , Andronescu Corina , Ostermann Rainer
Published: October 2024

In this study, we report highly crosslinked hybrid aerogels with an organic backbone based on vinylmethyldimethoxysilane (VMDMS) with tuneable properties. For an improved and highly reproducible synthesis, a prepolymer based on 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (D4V4) and VMDMS as monomers was prepared and purified. Di-tert-butylperoxide (DTBP) concentrations of 1 mol% initiate the radical polymerization of the mentioned monomers to achieve high yields of polymers. After purification, the obtained viscous polyorganosilane precursor could be reproducibly crosslinked with dimethyldimethoxysilane (DMDMS) or methyltrimethoxysilane (MTMS) to form gels in benzylic alcohol (BzOH), water (H2O) and tetramethylammonium hydroxide (TMAOH). Whereas freeze-drying these silica-based hybrid aerogels led to high thermal conductivity (>20 mW m−1K−1) and very fragile materials, useful aerogels were obtained via solvent exchange and supercritical drying with CO2. The DMDMS-based aerogels exhibit enhanced compressibility (31% at 7 kPa) and low thermal conductivity (16.5 mW m−1K−1) with densities around (0.111 g cm−3). The use of MTMS results in aerogels with lower compressibility (21% at 7 kPa) and higher density (0.124 g cm−3) but excellent insulating properties (14.8 mW m−1K−1).
ANKS6 Variants Underlie Polycystic Kidneys in Prenatal and Neonatal Cases
Authors: Almohlesy Lama S. , Imtiaz Faiqa , Tulbah Maha , Alhashem Amal , Alhajooj Manar , Alhashem Abdullah , Mabillard Holly , Sayer John A. , Alharbi Khalid K. , Al-Hamed Mohamed H.
Published: October 2024

Background: Nephronophthisis (NPHP) is an autosomal recessive genetic disorder that can cause early-onset kidney failure. ANKS6 plays an important role in early kidney development and encodes a protein that interacts with other proteins within the primary cilium. ANKS6 mutations are known to cause nephronophthisis 16 (NPHP-16). Little is known regarding fetal ultrasound imaging and the antenatal diagnosis of fetuses with ANKS6-associated kidney disease. Here, we report the detection of ANKS6 variants in consanguineous families with polycystic kidney antenatally and in the early stages of life. Methods: Three unrelated Saudi Arabian patients (two prenatal patients and one neonate) were investigated. These cases were referred to the hospital due to the presence of echogenic kidneys on antenatal scanning. After clinical and phenotypic evaluation, whole-exome sequencing (WES) was performed on the cord and peripheral blood to identify the molecular genetic causes associated with the echogenic kidney phenotypes. Results: Two homozygous sequence variants were detected in ANKS6. The homozygous missense novel variant ANKS6: c.1159A>C was detected in Families 1 and 2. In the third family, the known homozygous loss-of-function variant ANKS6: c.907+2T>A was detected. Conclusions: We identified homozygous ANKS6 variants in three families presenting with antenatal polycystic kidney disease. The findings provide an expanded clinical presentation of ANKS6 and emphasize the utility of WES in the diagnosis of echogenic kidneys in prenatal settings.
Geology, Archaeology, and Historical Studies of the Late 16th Century Plinian Eruption of Raung Volcano: A Potential Case for Disaster Geotourism in Ijen UNESCO Global Geopark, East Java, Indonesia
Authors: Sabila Firman Sauqi Nur , Abdurrachman Mirzam , Saepuloh Asep , Kurniawan Idham Andri , Baraas Abdillah , Yudiantoro Dwi Fitri , Kusdaryanto Hery
Published: October 2024

The enigmatic major eruption in the late 16th century, believed to have originated from Raung, the most active stratovolcano in the Ijen UNESCO Global Geopark in East Java, Indonesia, has ignited significant debate among researchers and historians due to its profound impact on the region. This research aims to substantiate Raung as the likely source of the major eruption by integrating geological, archaeological, and historical data. This study synthesizes current findings and explores ongoing debates surrounding historical volcanic activities. Eruption parameters suggest that the late 16th century eruption exhibited a Plinian type, characterized by an explosive eruption column reaching the stratosphere, widespread pumiceous tephra fallout, and pyroclastic density current (PDC). Stratigraphic succession reveals that the eruption occurred in five phases, with deposits from 10 eruptive units. These deposits are mainly concentrated on the northwestern flank of Raung. Archaeological findings, historical records, and local legends converge to pinpoint the occurrence of this catastrophic event in the late 16th century. These diverse sources estimate that the eruption resulted in approximately 10,000 casualties, marking it as one of the most significant volcanic disasters in the past 500 years. The implications of this eruption extend beyond historical documentation, providing a critical case study for advancing disaster mitigation strategies through geotourism in the geopark area. Moreover, the eruption record outcrops identified in this study can be proposed as potential new geosites within the Ijen UNESCO Global Geopark, enhancing its educational and touristic value. We propose the Jebung Kidul, Alas Sumur, and Batu Sappar sites as potential disaster-based geosites, considering that these sites record the eruption process and preserve archaeological structures. This addition would not only commemorate the historical event but also promote awareness and preparedness for future volcanic activities in the region.
Voluntary Neonatal Medication Incident Reporting—A Single Centre Retrospective Analysis
Authors: Nundeekasen Sunaina , McIntosh Joanne , McCleary Laurence , O’Neill Cathryn , Chaudhari Tejasvi , Abdel-Latif Mohamed E.
Published: October 2024

Background: Medication errors in neonatal intensive care units (NICUs) are prevalent, with dosage and prescription errors being the most common. Aims: To identify the common medication errors reported over twelve years using a voluntary, nonanonymous incident reporting system (RiskMan clinical incident reporting information system) at an Australian tertiary NICU. Methods: This was a single-centre cohort study conducted at a tertiary NICU. All medication-related incidents (errors) reported prospectively through the RiskMan online voluntary reporting database from January 2010 to December 2021 were included. The medication incidents were grouped into administration, prescription, pharmacy-related, and others, which included the remaining uncommon incidents. Results: Over the study period, 583 medication errors were reported, including administration-related (41.3%), prescription-related (24.5%), pharmacy-related (10.1%), and other errors (24%). Most incidents were reported by nursing and midwifery staff (77%) and pharmacists (17.5%). Most outcomes were minor or insignificant (98%), with only a few resulting in major or significant harm. There was one extreme incident that may have contributed to the death of a neonate and nine moderate incidents. Conclusions: Our results demonstrate that medication errors are common and highlight the need to support improvement initiatives and implement existing evidence-based interventions in routine practice to minimise medication errors in the NICU.
The Economics of Roman Construction in Bracara Augusta (Braga, Portugal): Building Stone Identification and Working Costs
Authors: Ribeiro Jorge , Carvalho Paula C. S. , Fragata Ana
Published: October 2024

Bracara Augusta (Braga, Portugal), one of the most important cities of the NW Iberian Peninsula, was founded by the emperor Augustus approximately between the years 16/15 BC. Throughout the Roman period, it has undergone various monumentalisation programmes according to its status, the first one as a convent capital and a later one as a provincial capital. The investigation of the economic cost of construction in the ancient world allows us to understand Roman architecture and the society of builders, both from economic and social perspectives. In recent years, based on the works of Janet DeLaine and Paolo Barresi, despite the large number of variables and the difficulty of quantifying them, a calculation methodology has been developed to approximate the cost of architecture, based on the information contained in two main documents: the Diocletian’s Edict of Prices (301 AD) and the nineteenth-century architecture and engineering manuals summarised by Pegoretti. Our goal is to understand part of the construction process of Roman architecture in the NW Iberian Peninsula, considering the cost of raw materials, namely stone, and the labour required in its preparation and utilisation. In this study, we present a cost proposal associated with the monumentalisation of two of the city’s important buildings: the forum’s large buildings and the theatre. The construction of the city was made fundamentally using local stone, from different quarries, endowing it with notable buildings and requiring a strong contribution from the local elites.
Optimizing Greenhouse Cucumber Fertigation Through Grafting: Improving Yield, Bioactive Compounds, and Antioxidant Activity
Authors: Vojnović Đorđe , Maksimović Ivana , Koprivica Gabrijela , Tepić Horecki Aleksandra , Milić Anita , Adamović Boris , Šumić Zdravko , Ilin Žarko
Published: October 2024

Consumers prefer cucumbers (Cucumis sativus) with high antioxidant content, which is often at odds with farmers’ goals of maximizing yield. Therefore, this study aims to explore new methods for fertigation and grafting to optimize the yield and quality of cucumbers. In a greenhouse experiment, we tested fertigation with three different nutrient solutions: the standard as a control (CF) and two new formulations (NF1 and NF2). We also examined grafting in three variants: non-grafted (CG), grafting onto Cucurbita moschata × Cucurbita moschata (G1), and grafting onto Lagenaria siceraria (G2). Our results showed that the highest increase in phenolic content in the flesh of cucumber was observed in the NF2 × G1 treatment (↑ 22.4%). In contrast, grafting and the new fertigation methods generally reduced the phenolic content in the peel. Grafting with G1 significantly increased flavonoid content in the flesh (↑ 59.4% and ↑ 77.3%) but significantly decreased it in the peel. The NF2 × G1 treatment achieved the most significant increases in antioxidant activity indicators, DPPH (↑ 25.9%) and FRAP (↑ 39.4%). For farmers seeking to achieve high yields of greenhouse cucumbers, the combination of NF1 × G1 is recommended, as it resulted in the highest yield increase (↑ 45.3%). Consumers are advised to eat cucumbers with the peel, as this study found higher levels of antioxidant compounds in the peel compared to the flesh.
Comparative Transcriptome Analysis of Non-Organogenic and Organogenic Tissues of Gaillardia pulchella Revealing Genes Regulating De Novo Shoot Organogenesis
Authors: Bansal Yashika , Mujib A. , Bansal Mahima , Mohsin Mohammad , Nafees Afeefa , Dewir Yaser Hassan
Published: October 2024

Gaillardia pulchella is an important plant species with pharmacological and ornamental applications. It contains a wide array of phytocompounds which play roles against diseases. In vitro propagation requires callogenesis and differentiation of plant organs, which offers a sustainable, alternative synthesis of compounds. The morphogenetic processes and the underlying mechanisms are, however, known to be under genetic regulation and are little understood. The present study investigated these events by generating transcriptome data, with de novo assembly of sequences to describe shoot morphogenesis molecularly in G. pulchella. The RNA was extracted from the callus of pre- and post-shoot organogenesis time. The callus induction was optimal using leaf segments cultured onto MS medium containing α-naphthalene acetic acid (NAA; 2.0 mg/L) and 6-benzylaminopurine (BAP; 0.5 mg/L) and further exhibited a high shoot regeneration/caulogenesis ability. A total of 68,366 coding sequences were obtained using Illumina150bpPE sequencing and transcriptome assembly. Differences in gene expression patterns were noted in the studied samples, showing opposite morphogenetic responses. Out of 10,108 genes, 5374 (53%) were downregulated, and there were 4734 upregulated genes, representing 47% of the total genes. Through the heatmap, the top 100 up- and downregulating genes’ names were identified and presented. The up- and downregulated genes were identified using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Important pathways, operative during G. pulchella shoot organogenesis, were signal transduction (13.55%), carbohydrate metabolism (8.68%), amino acid metabolism (5.11%), lipid metabolism (3.75%), and energy metabolism (3.39%). The synthesized proteins displayed phosphorylation, defense response, translation, regulation of DNA-templated transcription, carbohydrate metabolic processes, and methylation activities. The genes’ product also exhibited ATP binding, DNA binding, metal ion binding, protein serine/threonine kinase -, ATP hydrolysis activity, RNA binding, protein kinase, heme and GTP binding, and DNA binding transcription factor activity. The most abundant proteins were located in the membrane, nucleus, cytoplasm, ribosome, ribonucleoprotein complex, chloroplast, endoplasmic reticulum membrane, mitochondrion, nucleosome, Golgi membrane, and other organellar membranes. These findings provide information for the concept of molecular triggers, regulating programming, differentiation and reprogramming of cells, and their uses.
Low Nitrogen Availability in Organic Fertilizers Limited Organic Watermelon Transplant Growth
Authors: Liu Jun , Zhang Qianwen , Masabni Joseph , Niu Genhua
Published: October 2024

Fertilization guidelines for organic watermelon transplant production are rare. We investigated the effect of four commercial organic fertilizers and seven organic fertilizer blends, along with one conventional fertilizer (Peter’s Professional 20-20-20) on watermelon transplants. The four organic fertilizers were Nature Safe (fertilizer label: 7-7-7), Miracle-Gro (8-8-8), Dr. Earth fertilizer tea (4-4-4), and Drammatic (2-4-1). The seven blended organic fertilizers were created by supplementing Drammatic with nitrogen (N)-rich and/or potassium (K)-rich fertilizers to balance its N:phosphorus (P):K ratios. Watermelon ‘Jubilee’ was sown in organic substrate, and fertilizer treatments were applied weekly with a total of 0.4 g nitrogen/L substrate. Miracle-Gro and Drammatic had the highest N mineralization rate after 21 days and the highest inorganic N concentration, respectively, and resulted in the highest shoot dry weight among organic fertilizers. Miracle-Gro also resulted in the highest root dry weight. Dr. Earth fertilizer tea supplied the lowest N and P, and resulted in stunted transplants. Our results indicated that nitrate concentration was the most important factor influencing both shoot and root growth. Supplementing Drammatic with N-rich and/or K-rich fertilizers to balance its N:P:K ratio did not affect shoot and root dry weight. Combined, we concluded that nitrogen availability rather than nutrient balance is the key factor influencing watermelon transplant growth.
Exploring Resilience and Its Determinants in the Forced Migration of Ukrainian Citizens: A Psychological Perspective
Authors: Andrushko Yaryna , Lanza Stephanie T.
Published: October 2024

This study enhances the understanding of resilience in forced migration through a psychological lens, highlighting the importance of identifying resilience determinants and evidence-based interventions. By fostering resilience, policymakers and practitioners can support the well-being and adaptive capacities of forcibly displaced Ukrainians, promoting psychological recovery, social integration, and positive long-term outcomes for affected individuals and communities. To determine the key resilience indicators, survey data were collected in 2023 from n = 502 Ukrainian refugees living in the U.S. (M age = 27 years). Individuals reported various psychological factors and cultural experiences, revealing high resilience and low-stress tolerance among forced Ukrainian migrants in the U.S., along with a strong correlation between their adopted acculturation strategies and their resilience and levels of traumatization.