bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Security of the Dandelion Protocol
Authors: Goncalves Brian , Mashatan Atefeh
Published: March 2022

In this paper, we review the peer-to-peer blockchain transaction protocol, Dandelion, and develop an oracle-based model for its network and security. We formalize a series of security and functional criteria, such as unforgeability, non-repudiation, and immutability, into security experiments. In our model, we consider a quantum-capable adversary who seeks to undermine any of the security criteria while using oracles to simulate and interact with the Dandelion network. We then prove the security of Dandelion in our model with a series of (tight) security reductions as our main result. In addition, we prove that Dandelion is resistant to double-spending attacks.
Varieties of Selective Influence: Toward a More Complete Taxonomy and Implications for Systems Identification
Authors: Townsend James T. , Liu Yanjun
Published: March 2022

All science, including psychological science, is subject to what Townsend and Ashby have called the principle of correspondent change which ensures that experimental manipulations act as informed agents with respect to predictions and testing critical theoretical features. Mostly, this type of program goes unspoken. Within the general field known as the information processing approach, S. Sternberg invented the additive factors method in which the aforesaid feature plays a major and explicit role. We call this approach a theory driven methodology because the scientist formulates a set of theories or models and then formulates experimental variables that will permit strong tests among the hypothetical alternatives. Our term for the general approach is systems factorial technology. Often, these tests can be accomplished with qualitative, non-parametric, distribution free methods, but our so-called sieve method advocates, once the initial qualitative steps are accomplished, a move to assessing more detail parametric versions of the model classes. Over the decades, the meta-theory underpinning SFT and like approaches has evidenced dramatic growth in both expanse and depth. Particularly, the critical assumption of selective influence, testable to some extent, has received extensive and sophisticated treatment. The various central allied concepts are interlinked but do not form a simple linearly-ordered chain. This study carries on exploration of the central concepts and relationships and their implications for psychological research.
On the Stabilization of a Network of a Class of SISO Coupled Hybrid Linear Subsystems via Static Linear Output Feedback
Authors: De la Sen Manuel
Published: March 2022

This paper deals with the closed-loop stabilization of a network which consists of a set of coupled hybrid single-input single-output (SISO) subsystems. Each hybrid subsystem involves a continuous-time subsystem together with a digital (or, eventually, discrete-time) one being subject to eventual mutual couplings of dynamics and also to discrete delayed dynamics. The stabilizing controller is static and based on linear output feedback. The controller synthesis method is of algebraic type and based on the use of a linear algebraic system, whose unknown is a vector equivalent form of the controller gain matrix, which is obtained from a previous algebraic problem version which is based on the ad hoc use of the matrix Kronecker product of matrices. As a first step of the stabilization, an extended discrete-time system is built by discretizing the continuous parts of the hybrid system and to unify them together with its digital/discrete-time ones. The stabilization study via static linear output feedback contains several parts as follows: (a) stabilizing controller existence and controller synthesis for a predefined targeted closed-loop dynamics, (b) stabilizing controller existence and its synthesis under necessary and sufficient conditions based on the statement of an ad hoc algebraic matrix equation for this problem, (c) achievement of the stabilization objective under either partial or total decentralized control so that the whole controller has only a partial or null information about couplings between the various subsystems and (d) achievement of the objective under small coupling dynamics between subsystems.
A Novel Low-Query-Budget Active Learner with Pseudo-Labels for Imbalanced Data
Authors: Tharwat Alaa , Schenck Wolfram
Published: March 2022

Despite the availability of a large amount of free unlabeled data, collecting sufficient training data for supervised learning models is challenging due to the time and cost involved in the labeling process. The active learning technique we present here provides a solution by querying a small but highly informative set of unlabeled data. It ensures high generalizability across space, improving classification performance with test data that we have never seen before. Most active learners query either the most informative or the most representative data to annotate them. These two criteria are combined in the proposed algorithm by using two phases: exploration and exploitation phases. The former aims to explore the instance space by visiting new regions at each iteration. The second phase attempts to select highly informative points in uncertain regions. Without any predefined knowledge, such as initial training data, these two phases improve the search strategy of the proposed algorithm so that it can explore the minority class space with imbalanced data using a small query budget. Further, some pseudo-labeled points geometrically located in trusted explored regions around the new labeled points are added to the training data, but with lower weights than the original labeled points. These pseudo-labeled points play several roles in our model, such as (i) increasing the size of the training data and (ii) decreasing the size of the version space by reducing the number of hypotheses that are consistent with the training data. Experiments on synthetic and real datasets with different imbalance ratios and dimensions show that the proposed algorithm has significant advantages over various well-known active learners.
Improved Security of E-Healthcare Images Using Hybridized Robust Zero-Watermarking and Hyper-Chaotic System along with RSA
Authors: Jain Jaishree , Jain Arpit , Srivastava Saurabh Kumar , Verma Chaman , Raboaca Maria Simona , Illés Zoltán
Published: March 2022

With the rapid advancements of the internet of things (IoT), several applications have evolved with completely dissimilar structures and requirements. However, the fifth generation of mobile cellular networks (5G) is unable to successfully support the dissimilar structures and requirements. The sixth generation of mobile cellular networks (6G) is likely to enable new and unidentified applications with varying requirements. Therefore, 6G not only provides 10 to 100 times the speed of 5G, but 6G can also provide dynamic services for advanced IoT applications. However, providing security to 6G networks is still a significant problem. Therefore, in this paper, a hybrid image encryption technique is proposed to secure multimedia data communication over 6G networks. Initially, multimedia data are encrypted by using the proposed model. Thereafter, the encrypted data are then transferred over the 6G networks. Extensive experiments are conducted by using various attacks and security measures. A comparative analysis reveals that the proposed model achieves remarkably good performance as compared to the existing encryption techniques.
Recent Advances in the Treatment of Insulin Resistance Targeting Molecular and Metabolic Pathways: Fighting a Losing Battle?
Authors: Wolosowicz Marta , Prokopiuk Slawomir , Kaminski Tomasz W.
Published: March 2022

Diabetes Mellitus (DM) is amongst the most notable causes of years of life lost worldwide and its prevalence increases perpetually. The disease is characterized as multisystemic dysfunctions attributed to hyperglycemia resulting directly from insulin resistance (IR), inadequate insulin secretion, or enormous glucagon secretion. Insulin is a highly anabolic peptide hormone that regulates blood glucose levels by hastening cellular glucose uptake as well as controlling carbohydrate, protein, and lipid metabolism. In the course of Type 2 Diabetes Mellitus (T2DM), which accounts for nearly 90% of all cases of diabetes, the insulin response is inadequate, and this condition is defined as Insulin Resistance. IR sequela include, but are not limited to, hyperglycemia, cardiovascular system impairment, chronic inflammation, disbalance in oxidative stress status, and metabolic syndrome occurrence. Despite the substantial progress in understanding the molecular and metabolic pathways accounting for injurious effects of IR towards multiple body organs, IR still is recognized as a ferocious enigma. The number of widely available therapeutic approaches is growing, however, the demand for precise, safe, and effective therapy is also increasing. A literature search was carried out using the MEDLINE/PubMed, Google Scholar, SCOPUS and Clinical Trials Registry databases with a combination of keywords and MeSH terms, and papers published from February 2021 to March 2022 were selected as recently published papers. This review paper aims to provide critical, concise, but comprehensive insights into the advances in the treatment of IR that were achieved in the last months.
Pregnancy in Congenital Heart Disease, Complicated by Pulmonary Arterial Hypertension—A Challenging Issue for the Pregnant Woman, the Foetus, and Healthcare Professionals
Authors: Rudienė Virginija , Kaplerienė Lina , Jančauskaitė Dovilė , Meškėnė Emilija , Palevičiūtė Eglė , Laukytė-Slėnienė Monika , Gasiūnaitė Diana , Ramašauskaitė Diana , Jurevičienė Elena , Gumbienė Lina
Published: March 2022

Background and Objectives: Pregnancy and delivery in patients with congenital heart disease (CHD) and pulmonary arterial hypertension (PAH) carry a very high risk for maternal and foetal complications and are contraindicated according to the guidelines. In the last decades, when an available modern PAH-targeted medication therapy and a new management concept improved patients’ well-being and survival, some PAH-CHD females decided to conceive. Of note, despite advanced treatment and modern healthcare system possibilities, dealing with pregnancy in a diverse PAH-CHD population is still challenging. The study aimed to share our experience with PAH-CHD pregnancies and discuss the risk assessment and current management of these patients with the combination of two rare diseases. Materials and Methods: The retrospective search of pulmonary hypertension and adult CHD registries in our hospital was performed, selecting all patients with CHD and PAH who conceived pregnancy from 2013 to 2021. Baseline demographic, clinical, and functional characteristics and clinical outcomes were collected. Results: Thirteen pregnancies in eight patients with PAH-CHD resulted in seven live births, three miscarriages, and three terminations. Five women were diagnosed with Eisenmenger syndrome (ES) and three with residual PAH after CHD repair. Before pregnancy, half of them were in WHO functional class III. Seven (87.5%) patients received targeted PAH treatment with sildenafil during pregnancy. In addition, the two most severe cases were administered with iloprost during peripartum. Three ES patients delivered preterm by Caesarean section under general anaesthesia. No neonatal mortality was reported. Maternal complications were observed in half of our cases. One patient died 12 days after the delivery in another hospital due to deterioration of heart failure. Conclusions: On the basis of our clinical experience, we conclude that pregnancy and delivery carry a high risk for maternal complications and should be avoided in women with PAH-CHD. The individualised approach of multidisciplinary care and appropriate monitoring are mandatory in reducing the risk of adverse outcomes.
MicroRNA as Candidate Biomarkers in Atypical Parkinsonian Syndromes: Systematic Literature Review
Authors: Bougea Anastasia
Published: March 2022

Background and Objectives: Multiple system atrophy (MSA) and progressive supranuclear palsy (PSP) are rare atypical parkinsonian syndromes, characterized by motor and cognitive symptoms. Their clinical diagnosis is challenging because there are no established biomarkers. Dysregulation of microRNAs (miRNAs/miRs) has been reported to serve an important role in neurodegenerative diseases. However, the miRNA profiles of MSA and PSP patients are rarely reported. The aim of this study was to critically review the role of miRNAs as diagnostic biomarkers to differentiate these atypical parkinsonian disorders and their role in disease pathogenesis. Materials and Methods: A systematic literature search of PubMed was conducted up to February 2022 according the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: A total of 15 studies were analyzed. Three studies have shown that miR-9-3p, miR-19a, miR-19b, and miR-24 are potential biomarkers for MSA. In two studies, miR-132 was downregulated, whereas miR-147a and miR-518e were upregulated in the brain tissue of PSP patients. Conclusions: The potential of miRNA is still uncertain as a potential differential diagnostic marker to identify these disorders. Pre-analytical and analytical factors of included studies were important limitations to justify the introduction of miRNAs into clinical practice.
Investigation of the Resistivity and Emissivity of a Pellicle Membrane for EUV Lithography
Authors: Wi Seong Ju , Jang Yong Ju , Kim Haneul , Cho Kyeongjae , Ahn Jinho
Published: March 2022

A pellicle is a thin membrane structure that protects an extreme ultraviolet (EUV) mask from contamination during the exposure process. However, its limited transmittance induces unwanted heating owing to the absorption of EUV photons. The rupture of the EUV pellicle can be avoided by improving its thermal stability, which is achieved by improving the emissivity of the film. However, the emissivity data for thin films are not easily available in the literature, and its value is very sensitive to thickness. Therefore, we investigated the dependence of emissivity on structural parameters, such as thickness, surface roughness, and grain size. We found a correlation between resistivity and emissivity using theoretical and experimental approaches. By changing the grain size of the Ru thin film, the relationship between resistivity and emissivity was experimentally verified and confirmed using the Lorentz–Drude model. Finally, we present a method to develop an EUV pellicle with better thermal stability that can withstand high-power EUV light sources.
Prediction of Work Hardening in Bearing Steels Undergoing Rolling Contact Loading with a Dislocation-Based Model
Authors: Yin Hongxiang , Bai Xue , Fu Hanwei
Published: March 2022

The work hardening behaviour of GCr15 bearing steel during rolling contact fatigue (RCF) is investigated. Ball-on-rod RCF tests and micro-indentation tests are performed to obtain various subsurface hardness profiles in rod specimens. It is found that orthogonal shear stress is responsible for work hardening under Hertzian contact and that the extent of hardness increase is positively associated with the stress level and number of cycles. A dislocation-based work hardening model is established by combining the Kocks–Mecking theory, the bearing steel plasticity equation and the Taylor relation. The proposed model is capable of predicting hardness changes with any given rolling contact stress state and number of cycles. The modelling results are compared against the experimental results, with good agreement obtained. This research also provides a methodology for studying the work hardening of different types of bearing steels undergoing RCF, from experiment to modelling.
A Review of Corrosion under Insulation: A Critical Issue in the Oil and Gas Industry
Authors: Cao Qing , Pojtanabuntoeng Thunyaluk , Esmaily Marco , Thomas Sebastian , Brameld Michael , Amer Ayman , Birbilis Nick
Published: March 2022

Corrosion under insulation (CUI) is defined as any form of external corrosion that occurs on the underlying metal beneath insulated equipment, due to water ingress through the insulation layer. This type of corrosion is frequently observed in oil and gas production, where insulated piping is prevalent, and has historically remained a predominant materials integrity issue. The prediction and direct visualisation of CUI are challenging tasks because of the coverage of the insulation layer(s) and any external jacketing or cladding. Several factors, including the local/ambient environment, system design, and the piping installation process, can influence how CUI initiates and propagates. In this review, CUI background, CUI monitoring, and CUI mitigation strategies are discussed.
Surface Laser Treatment of Cast Irons: A Review
Authors: Catalán Néstor , Ramos-Moore Esteban , Boccardo Adrian , Celentano Diego
Published: March 2022

Heat treatments are frequently used to modify the microstructure and mechanical properties of materials according to the requirements of their applications. Laser surface treatment (LST) has become a relevant technique due to the high control of the parameters and localization involved in surface modification. It allows for the rapid transformation of the microstructure near the surface, resulting in minimal distortion of the workpiece bulk. LST encompasses, in turn, laser surface melting and laser surface hardening techniques. Many of the works devoted to studying the effects of LST in cast iron are diverse and spread in several scientific communities. This work aims to review the main experimental aspects involved in the LST treatment of four cast-iron groups: gray (lamellar) cast iron, pearlitic ductile (nodular) iron, austempered ductile iron, and ferritic ductile iron. The effects of key experimental parameters, such as laser power, scanning velocity, and interaction time, on the microstructure, composition, hardness, and wear are presented, discussed, and overviewed. Finally, we highlight the main scientific and technological challenges regarding LST applied to cast irons.
Late-Stage Glioma Is Associated with Deleterious Alteration of Gut Bacterial Metabolites in Mice
Authors: Herbreteau Aglae , Aubert Philippe , Croyal Mikaël , Naveilhan Philippe , Billon-Crossouard Stéphanie , Neunlist Michel , Delneste Yves , Couez Dominique , Aymeric Laetitia
Published: March 2022

Brain-gut axis refers to the bidirectional functional connection between the brain and the gut, which sustains vital functions for vertebrates. This connection also underlies the gastrointestinal (GI) comorbidities associated with brain disorders. Using a mouse model of glioma, based on the orthotopic injection of GL261 cell line in syngeneic C57BL6 mice, we show that late-stage glioma is associated with GI functional alteration and with a shift in the level of some bacterial metabolites in the cecum. By performing cecal content transfer experiments, we further show that cancer-associated alteration in cecal metabolites is involved in end-stage disease progression. Antibiotic treatment results in a slight but significant delay in mice death and a shift in the proportion of myeloid cells in the brain tumor environment. This work rationally considers microbiota modulating strategies in the clinical management of patients with late-stage glioma.
Varied Composition and Underlying Mechanisms of Gut Microbiome in Neuroinflammation
Authors: Farooq Rai Khalid , Alamoudi Widyan , Alhibshi Amani , Rehman Suriya , Sharma Ashish Ranjan , Abdulla Fuad A.
Published: March 2022

The human gut microbiome has been implicated in a host of bodily functions and their regulation, including brain development and cognition. Neuroinflammation is a relatively newer piece of the puzzle and is implicated in the pathogenesis of many neurological disorders. The microbiome of the gut may alter the inflammatory signaling inside the brain through the secretion of short-chain fatty acids, controlling the availability of amino acid tryptophan and altering vagal activation. Studies in Korea and elsewhere highlight a strong link between microbiome dynamics and neurocognitive states, including personality. For these reasons, re-establishing microbial flora of the gut looks critical for keeping neuroinflammation from putting the whole system aflame through probiotics and allotransplantation of the fecal microbiome. However, the numerosity of the microbiome remains a challenge. For this purpose, it is suggested that wherever possible, a fecal microbial auto-transplant may prove more effective. This review summarizes the current knowledge about the role of the microbiome in neuroinflammation and the various mechanism involved in this process. As an example, we have also discussed the autism spectrum disorder and the implication of neuroinflammation and microbiome in its pathogenesis.
Pseudomonas aeruginosa PAO1 Is Attracted to Bovine Bile in a Novel, Cystic Fibrosis-Derived Bronchial Epithelial Cell Model
Authors: Behroozian Shekooh , Sampedro Inmaculada , Dhodary Basanta , Her Stephanie , Yu Qianru , Stanton Bruce A. , Hill Jane E.
Published: March 2022

Cystic fibrosis (CF) is a life-threatening, inherited, multi-organ disease that renders patients susceptible throughout their lives to chronic and ultimately deteriorating protracted pulmonary infections. Those infections are dominated in adulthood by the opportunistic pathogen, Pseudomonas aeruginosa (Pa). As with other advancing respiratory illnesses, people with CF (pwCF) also frequently suffer from gastroesophageal reflux disease (GERD), including bile aspiration into the lung. GERD is a major co-morbidity factor in pwCF, with a reported prevalence of 35–81% in affected individuals. Bile is associated with the early acquisition of Pa in CF patients and in vitro studies show that it causes Pa to adopt a chronic lifestyle. We hypothesized that Pa is chemoattracted to bile in the lung environment. To evaluate, we developed a novel chemotaxis experimental system mimicking the lung environment using CF-derived bronchial epithelial (CFBE) cells which allowed for the evaluation of Pa (strain PAO1) chemotaxis in a physiological scenario superior to the standard in vitro systems. We performed qualitative and quantitative chemotaxis tests using this new experimental system, and microcapillary assays to demonstrate that bovine bile is a chemoattractant for Pa and is positively correlated with bile concentration. These results further buttress the hypothesis that bile likely contributes to the colonization and pathogenesis of Pa in the lung, particularly in pwCF.
The Antimicrobial Photoinactivation Effect on Escherichia coli through the Action of Inverted Cationic Porphyrin–Cyclodextrin Conjugates
Authors: Ribeiro Cláudia P. S. , Faustino Maria A. F. , Almeida Adelaide , Lourenço Leandro M. O.
Published: March 2022

Photodynamic action has been used for diverse biomedical applications, such as treating a broad range of bacterial infections. Based on the combination of light, dioxygen, and photosensitizer (PS), the photodynamic inactivation (PDI) approach led to the formation of reactive oxygen species (ROS) and represented a non-invasive, non-toxic, repeatable procedure for pathogen photoinactivation. To this end, different tetrapyrrolic macrocycles, such as porphyrin (Por) dyes, have been used as PSs for PDI against microorganisms, mainly bacteria. Still, there is significant room for improvement, especially new PS molecules. Herein, unsymmetrical new pyridinone (3–5) and thiopyridyl Pors (7) were prepared with α-, β-, or γ-cyclodextrin (CD) units, following their quaternization to perform the corresponding free-base Pors (3a–5a and 7a), and were compared with the already-known Pors 6a and 8a, both bearing thiopyridinium and CD units. These water-soluble porphyrins were evaluated as PSs, and their photophysical and photochemical properties and photodynamic effects on E. coli were assessed. The presence of one CD unit and three positive charges on the Por structure (3a–5a and 7a) enhanced their aqueous solubility. The photoactivity of the cationic Pors 3a–5a and 6a–8a ensured their potential against the Gram-negative bacterium E. coli. Within each series of methoxypyridinium vs thiopyridinium dyes, the best PDI efficiency was achieved for 5a with a bacterial viability reduction of 3.5 log10 (50 mW cm−2, 60 min of light irradiation) and for 8a with a total bacterial viability reduction (>8 log10, 25 mW cm−2, 30 min of light irradiation). Here, the presence of the methoxypyridinium units is less effective against E. coli when compared with the thiopyridinium moieties. This study allows for the conclusion that the peripheral charge position, quaternized substituent type/CD unit, and affinity to the outer bacterial structures play an important role in the photoinactivation efficiency of E. coli, evidencing that these features should be further addressed in the pursuit for optimised PS for the antimicrobial PDI of pathogenic microorganisms.
Metasomatism by Boron-Rich Fluids along Permian Low-Angle Normal Faults (Central Southern Alps, N Italy)
Authors: Zanchetta Stefano , Locchi Sofia , Carminati Gregorio , Mancuso Manuel , Montemagni Chiara , Zanchi Andrea
Published: March 2022

Low-Angle Normal Faults (LANFs) represent in the central Southern Alps area (N Italy) the main structures along which the Variscan basement is in contact with the Upper Carboniferous-Permian volcanic-sedimentary succession. Tourmalinites frequently occur along LANFs, usually replacing former cataclasites. The mineralogy and chemical composition of tourmalinites point to a metasomatic origin. LANFs, together with high-angle faults, controlled the opening of the Permian Orobic Basin and likely acted as a preferred pathway for hydrothermal fluids that triggered the Boron-metasomatism. Along the Aga-Vedello LANF, tourmalinites appear to have formed after the cessation of fault activity, as no brittle post-metasomatism deformation overprint has been observed. These relationships suggest that the circulation of B-rich fluids occurred after the opening of the Orobic Basin that is broadly constrained to the Early Permian. At the same time, ca. 285–270 Ma, a strong magmatic activity affected all the Southern Alps, ranging in composition from mafic to acidic rocks and from intrusions at deep crustal levels to effusive volcanic products. The Early Permian magmatism was likely the source of the late-stage hydrothermal fluids that formed the tourmalinites. The same fluids could also have played a significant role in the formation of the Uranium ore deposit of the Novazza-Vedello mining district, as the ore bodies in the Vedello valley are concentrated along the basement-cover contact.
Field Studies on the Effect of Bioaugmentation with Bacillus amyloliquefaciens FZB42 on Plant Accumulation of Rare Earth Elements and Selected Trace Elements
Authors: Okoroafor Precious Uchenna , Ikwuka God’sfavour , Zaffar Nazia , Ngalle Epede Melvice , Mensah Martin Kofi , Haupt Johann , Golde Andreas , Heilmeier Hermann , Wiche Oliver
Published: March 2022

This study is an investigation of the effect of soil bioaugmentation (inoculation) on a field scale with the commercially available product RhizoVital®42, containing Bacillus amyloliquefaciens FZB4, on element bioavailability, plant biomass production, as well as accumulation of rare earth elements (REEs), germanium, and selected trace elements. Zea mays and Helianthus annuus were selected as test plants. Post-harvest, results showed inoculation increased biomass production of Z. mays and H. annuus by 24% and 26%, albeit insignificant at p ≤ 0.05. Bioaugmentation enhanced Z. mays shoot content of P, Cd, and Ge by percentages between 73% and 80% (significant only for Ge) and decreased shoot content of REET, Pb, and Cu by 28%, 35%, and 59%, respectively. For H. annuus grown on bioaugmented soil, shoot content of Ca, Cu, Ge, REET, and Pb increased by over 40%, with a negligible decrease observed for Cd. Summarily, results suggest that bioaugmentation with Bacillus amyloliquefaciens FZB42 could enhance biomass production, increase soil element bioavailability enhance, and increase or reduce plant accumulation of target elements. Additionally, differences in P use efficiency could influence bioaugmentation effects on P accumulation.
Estimation of the Ambient Temperatures during the Crystallization of Halite in the Oligocene Salt Deposit in the Shulu Sag, Bohaiwan Basin, China
Authors: Zhao Xianfu , Zhao Yanjun , Wang Mingquan , Hu Yufei , Liu Chenglin , Zhang Hua
Published: March 2022

The centimeter-scale halite rhythmites in the first member of the Shahejie Formation in the Shulu Sag of the Bohai Bay Basin are investigated, and the Eocene to early Oligocene paleoenvironmental characteristics of a typical saline lake basin are restored by reconstructing the temperature and compositional information of ancient brines. The obtained homogenization temperatures (Th) of fluid inclusions range from 6.5 to 49.2 °C, with a relative lower Th from transparent halite samples than from gray halite samples. This suggests different temperature conditions and a probable association with seasonal changes. The ion contents of halite fluid inclusions reveal the lake brine is a Na-Mg-K-Ca-Cl type and reached the initial stage of halite deposition. The transparent halite samples plotted within different phase regions than the gray halite samples on plots of ion contents and showed significant change within phase regions. Combined with the observed cm-scale rhythm in the evaporite sequences of the Shulu Sag, these results suggest a shallow water environment and frequent dilution by inflows of fresher water caused by seasonal climate change. The gray halite formed under higher temperatures and increased inflow conditions, and the transparent halite formed under lower temperatures and decreased inflow conditions. Compared with the Jiangling Sag in Hubei Province in southern China, the Shulu Sag may have been less affected by igneous rocks in the Es1 Formation due to the material source, and the concentration of trace elements such as lithium, strontium and boron in the ancient salt lake brine was lower.
New Geochemical Framework and Geographic Information System Methodologies to Assess Element Occurrence, Persistence, and Mobility in Groundwater and Surface Water
Authors: Blake Johanna M. , Walton-Day Katherine , Gallegos Tanya J. , Yager Douglas B. , Teeple Andrew , Humberson Delbert , Stengel Victoria , Becher Kent
Published: March 2022

This study presents a geochemical framework and geographic information system (GIS) method for assessing the intrinsic potential of surface water and groundwater to mobilize arsenic, molybdenum, selenium, uranium, and vanadium. The method was created using published groundwater and surface water geochemical data from the National Uranium Resource Evaluation database for 2302 groundwater and 915 surface water samples. The method was evaluated using published groundwater geochemical data from the Texas Water Development Board. Geochemical data were analyzed in GIS. Samples were categorized by environmental condition, which was determined by using reduction–oxidation—as indicated by pe—and pH ranges for each sample based on geochemical mobility frameworks developed by Smith (2007) and Perel’man (1986). Reduction–oxidation and pH influence the occurrence, persistence, and mobility of arsenic, molybdenum, selenium, uranium, and vanadium in groundwater and surface water. Reduction–oxidation categories were assigned to water samples using concentrations of redox-active constituents, including dissolved oxygen, iron, manganese, and sulfur. The presence of iron substrates and hydrogen sulfides were considered in relation to mobility mechanisms. Twelve-digit hydrologic unit code (HUC) boundaries were used in GIS as analysis areas to determine the most commonly occurring environmental condition in each HUC. The resulting maps identify the environmental conditions in different areas that can be used to identify where the elements are mobile. This methodology provides a systematic approach to identify areas where elements in groundwater and surface water may occur and persist and may be transferable to other locations.
Evaluation of Anti-Inflammatory and Antioxidant Effectsof Chrysanthemum Stem and Leaf Extract on Zebrafish Inflammatory Bowel Disease Model
Authors: Li Yi , Liu Xia-Jin , Su Shu-Lan , Yan Hui , Guo Sheng , Qian Da-Wei , Duan Jin-Ao
Published: March 2022

Present studies have shown that Flos Chrysanthemi has anti-inflammatory and other effects and regulates intestinal function, while the chrysanthemum stem and leaf as non-medicinal parts of chrysanthemum have similar chemical components with chrysanthemum, but the activity and mechanisms are rarely elucidated. Therefore, this study used a DSS-induced zebrafish inflammatory bowel disease model to study the anti-inflammatory and antioxidant effects of chrysanthemum stem and leaf extracts. The results indicate that DSS induction leads to increased secretion of acidic mucin in the intestines of juvenile fish, enlargement of the intestinal lumen and the emergence of intestinal inflammation. Compared with the model group, each administration group differentially inhibited the expression of IL-1β, IL-8 and MMP9 in DSS-induced zebrafish, while upregulating the activity of superoxide dismutase. The quantitative analysis results showed that the flavonoids (including Linarin, Diosmetin-7-glucoside, Tilianin, etc.) and phenolic acids (including Isochlorogenic acid C, Isochlorogenic acid A, 1,3-Dicaffeoylquinic acid, etc.) in the alcohol extract were closely related with both anti-inflammatory and antioxidant activity, while the polysaccharides were also shown a certain anti-inflammatory and antioxidant activity. In conclusion, this study suggests that the flavonoids, phenolic acids and polysaccharides from chrysanthemum stem and leaf extracts can improve inflammatory bowel disease of zebrafish by regulating the expressions of IL-1β, IL-8 and MMP9.
Schisandra rubriflora Fruit and Leaves as Promising New Materials of High Biological Potential: Lignan Profiling and Effect-Directed Analysis
Authors: Sobstyl Ewelina , Szopa Agnieszka , Dziurka Michał , Ekiert Halina , Nikolaichuk Hanna , Choma Irena Maria
Published: March 2022

The effect-directed detection (EDD) of Schisandra rubriflora fruit and leaves extracts was performed to assess their pharmacological properties. The EDD comprised TLC—direct bioautography against Bacillus subtilis, a DPPH assay, as well as α-glucosidase, lipase, tyrosinase, and acetylcholinesterase (AChE) inhibition assays. The leaf extracts showed stronger antioxidant activity than the fruit extract as well as inhibition of tyrosinase and lipase. The fruit extract was found to be extremely active against B. subtilis and to inhibit α-glucosidase and AChE slightly more than the leaf extracts. UHPLC–MS/MS analysis was carried out for the bioactive fractions and pointed to the possible anti-dementia properties of the dibenzocyclooctadiene lignans found in the upper TLC fractions. Gomisin N (518 mg/100 g DW), schisanhenol (454 mg/100 g DW), gomisin G (197 mg/100 g DW), schisandrin A (167 mg/100 g DW), and gomisin O (150 mg/100 g DW) were the quantitatively dominant compounds in the fruit extract. In total, twenty-one lignans were found in the bioactive fractions.
Effect of Molar Substitution on the Properties of γ-Hydroxypropyl Starch
Authors: Liu Xue-Li , Chen Yi-Fan , Yang Jing-Jing , Li Si-Jin , Xie Hua-Le , Ma Tian-Lin
Published: March 2022

A new type of hydroxyalkyl starch, γ-hydroxypropyl starch (γ-HPS), was prepared by etherification of alkali-activated starch with 3-chloropropanol. The reaction efficiency, morphological change, thermodynamic and apparent viscosity properties, and other physicochemical characteristics were described. The molar substitution (MS) of modified whole starch was determined to be 0.008, 0.017, 0.053, 0.106, and 0.178, with a ratio of 5%, 15%, 25%, 35%, and 45% 3-chloropropanol to starch (v/w), respectively. Compared to native starch, the granular size and shape and the X-ray diffraction pattern of γ-HPS are not very different. For low-substituted γ-HPS, the implications may be less evident. Thermal stability measurements by means of thermogravimetric analyses and differential scanning calorimetry (TGA-DSC) proved that thermal stability was reduced and water retaining capacity was increased after hydroxypropylation. Furthermore, the findings also showed that the solubility, light transmittance, and retrogradation of γ-HPS pastes could be improved by etherification. The greater the MS of the γ-HPS, the more its freeze–thaw stability and acid resistivity increased. In this study, we provide relevant information for the application of γ-HPS in food and non-food industries.
Design, Synthesis, and Evaluation of Novel 2H-Benzo[b][1,4]thiazin-3(4H)-one Derivatives as New Acetylcholinesterase Inhibitors
Authors: Haji Ali Sazan , Osmaniye Derya , Sağlık Begüm Nurpelin , Levent Serkan , Özkay Yusuf , Kaplancıklı Zafer Asım
Published: March 2022

Alzheimer’s disease (AD) is a slowly progressive neurodegenerative disease that causes dementia in people aged 65 and over. In the present study, a series of thiadiazole hybrid compounds with benzothiazine derivatives as acetylcholinesterase inhibitors were developed and evaluated for their biological activity. The AChE and BChE inhibition potentials of all compounds were evaluated by using the in vitro Ellman method. The biological evaluation showed that compounds 3i and 3j displayed significant inhibitory activity against AChE. Compounds 3i and 3j showed IC50 values of 0.027 µM and 0.025 µM against AChE, respectively. The reference drug donepezil (IC50 = 0.021 µM) also showed significant inhibition against AChE. Further docking simulation also revealed that these compounds (3i and 3j) interacted with the active site of the enzyme similarly to donepezil. The antioxidant study revealed that compounds 3i and 3j exhibited greater antioxidant effects. An in vitro blood–brain barrier permeability study showed that compounds 3i and 3j are promising compounds against AD. The cytotoxicity study of compounds 3i and 3j showed non-cytotoxic with an IC50 value of 98.29 ± 3.98 µM and 159.68 ± 5.53 µM against NIH/3T3 cells, respectively.
Heteroctanuclear Au4Ag4 Cluster Complexes of 4,5-Diethynylacridin-9-One with Luminescent Mechanochromism
Authors: Xie Pei , Wang Jin-Yun , Huang Ya-Zi , Wu Xue-Meng , Chen Zhong-Ning
Published: March 2022

Two heteroctanuclear Au4Ag4 cluster complexes of 4,5-diethynylacridin-9-one (H2L) were prepared through the self-assembly reactions of [Au(tht)2](CF3SO3), Ag(tht)(CF3SO3), H2L and PPh3 or PPh2Py (2-(diphenylphosphino)pyridine). The Au4Ag4 cluster consists of a [Au4L4]4− and four [Ag(PPh3)]+ or [Ag(PPh2Py)]+ units with Au4L4 framework exhibiting a twisted paper clip structure. In CH2Cl2 solutions at ambient temperature, both compounds show ligand fluorescence at ca. 463 nm as well as phosphorescence at 650 nm for 1 and 630 nm for 2 resulting from admixture of 3IL (intraligand) of L ligand, 3LMCT (from L ligand to Au4Ag4) and 3MC (metal-cluster) triplet states. Crystals or crystalline powders manifest bright yellow-green phosphorescence with vibronic-structured emission bands at 530 (568sh) nm for complex 1 and 536 (576sh) nm for complex 2. Upon mechanical grinding, yellow-green emission in the crystalline state is dramatically converted to red luminescence centered at ca. 610 nm with a drastic redshift of the emission after crystal packing is destroyed.
Metal-Organic Framework Assembled on Oriented Nanofiber Arrays for Field-Effect Transistor and Gas Sensor-Based Applications
Authors: Mu Jinlong , Zhong Xing , Dai Wei , Pei Xin , Sun Jian , Zhang Junyuan , Luo Wenjun , Zhou Wei
Published: March 2022

Metal–organic framework (MOF) films are essential for numerous sensor and device applications. However, metal-organic framework materials have poor machinability due to their predominant powder-like nature, and their presence as the active layer in a device can seriously affect the performance and utility of the device. Herein, active layers of field-effect transistor (FETs) devices and chemiresistor gas sensors with high performance were constructed by loading Cu3(HITP)2 (HITP = 2,3,6,7,10,11-hexaiminotriphenylene) in situ-axial anchoring on oriented nanofiber arrays prepared via electrospinning. The strong interaction between polar groups on the polymer chains and metal ions promotes the nucleation of Cu3(HITP)2, steric hindrance makes particles of Cu3(HITP)2 with uniform size, morphology, and good crystallinity during nucleation by liquid phase epitaxial growth (LPE). Influences of differently-oriented Cu3(HITP)2 NFAs-based FETs on the electrical properties were studied, optimally oriented Cu3(HITP)2 NFAs-based FETs showed good mobility of 5.09 cm2/V·s and on/off ratio of 9.6 × 103. Moreover, excellent gas sensing response characteristics were exhibited in sensing volatile organic compounds (VOCs). Chemiresistor gas sensors with high response value, faster response and recovery are widely suited for VOCs. It brings new inspirations for the design and utilization of electrically conductive MOFs as an active layer for FETs and sensor units for chemiresistor gas sensors.
In Vitro Alpha-Glucosidase Inhibitory Activity and the Isolation of Luteolin from the Flower of Gymnanthemum amygdalinum (Delile) Sch. Bip ex Walp.
Authors: Vonia Sheppriola , Hartati Rika , Insanu Muhamad
Published: March 2022

Diabetes mellitus is a major health issue that has posed a significant challenge over the years. Gymnanthemum amygdalinum is a well-known plant that can be potentially used to treat this disease. Therefore, this study aimed to evaluate the inhibitory effect of its root, stem bark, leaves, and flower extracts on alpha-glucosidase using an in vitro inhibition assay to isolate the bioactive compounds and determine their levels in the samples. The air-dried plant parts were extracted by maceration using methanol. The results showed that the flower extract had the greatest inhibitory effect (IC50 47.29 ± 1.12 µg/mL), followed by the leaves, roots, and stem bark. The methanolic flower extract was further fractionated with different solvents, and the ethyl acetate fraction showed the strongest activity (IC50 19.24 ± 0.12 µg/mL). Meanwhile, acarbose was used as a positive control (IC50 73.36 ± 3.05 µg/mL). Characterization based on UV, 1H-, and 13C-NMR established that the ethyl acetate fraction yielded two flavonoid compounds, namely, luteolin and 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3-methoxy-4H-chromen-4-on, which had IC50 values of 6.53 ± 0.16 µg/mL and 39.95 ± 1.59 µg/mL, respectively. The luteolin levels in the crude drug, methanolic extract, and ethyl acetate fraction were 3.4 ± 0.2 mg (0.3%), 32.4 ± 0.8 mg (3.2%), and 68.9 ± 3.4 mg (6.9%) per 1 g samples, respectively. These results indicated that the G. amygdalinum flower extract exerted potent inhibitory alpha-glucosidase activity.
Comparative Hydrodynamic Study on Non-Aqueous Soluble Archaeological Wood Consolidants: Butvar B-98 and PDMS-OH Siloxanes
Authors: Cutajar Michelle , Stockman Robert A. , Braovac Susan , Steindal Calin Constantin , Zisi Angeliki , Harding Stephen E.
Published: March 2022

Butvar B-98 and PDMS-OH both have a demonstrable ability as consolidants for archaeological wood. This makes them both potential treatment options for the Oseberg collection, which is one of the most important archaeological finds from the Viking era. Both Butvar B-98 and PDMS-OH are soluble in organic solvents, offering a useful alternative to aqueous-based consolidants. Extensive characterisation studies were carried out on both of these polymers, with the use of analytical ultracentrifugation and viscometry, for the benefit of conservators wanting to know more about the physical properties of these materials. Short column sedimentation equilibrium analysis using SEDFIT-MSTAR revealed a weight-average molar mass (weight-average molecular weight) Mw of (54.0 ± 1.5) kDa (kg · mol−1) for Butvar B-98, while four samples of PDMS-OH siloxanes (each with a different molar mass) had an Mw of (52.5 ± 3.0) kDa, (38.8 ± 1.5) kDa, (6.2 ± 0.7) kDa and (1.6 ± 0.1) kDa. Sedimentation velocity confirmed that all polymers were heterogeneous, with a wide range of molar masses. All molecular species showed considerable conformational asymmetry from measurements of intrinsic viscosity, which would facilitate networking interactions as consolidants. It is anticipated that the accumulated data on these two consolidants will enable conservators to make a more informed decision when it comes to choosing which treatment to administer to archaeological artefacts.
Simultaneous Determination of Multi-Class Pesticide Residues and PAHs in Plant Material and Soil Samples Using the Optimized QuEChERS Method and Tandem Mass Spectrometry Analysis
Authors: Słowik-Borowiec Magdalena , Szpyrka Ewa , Książek-Trela Paulina , Podbielska Magdalena
Published: March 2022

New analytical approaches to the simultaneous identification and quantification of 94 pesticides and 13 polycyclic aromatic hydrocarbons (PAHs) in five representative matrices (pepper, apple, lettuce, wheat, and soil) were developed. The analyses were based on gas chromatography coupled with triple quadrupole tandem mass spectrometry (GC-MS/MS). The procedure was optimized by changing the solvent used during the extraction, from acetonitrile to the acetone: n-hexane mixture at a volume ratio of 1:4 (v/v), as well as the use of a reduced amount of water during the extraction of compounds from cereals. An additional modification was the use of florisil instead of GCB in the sample cleanup step. A full method validation study was performed, at two concentration levels (LOQ and 1000 × LOQ), which showed satisfactory results for all analytes from the PAHs group, with recoveries ranging from 70.7–115.1%, and an average RSD of 3.9%. Linearity was tested in the range of 0.001–1.000 mg/kg and showed coefficients of determination (R2) ≥ 0.99 for all PAHs. Satisfactory recovery and precision parameters (LOQ and 100 × LOQ) were achieved for almost all analytes from the pesticide group in the range of 70.1–119.3% with the mean RSD equal to 5.9%. The observed linearity for all analytes in the concentration range of 0.005–1.44 mg/kg was R2 ≥ 0.99, with the exception of famoxadone, chizalofop-p-ethyl, prothioconazole, spirodiclofen, tefluthrin, and zoxamid. The extended uncertainties were estimated, using a top-down approach of 9.9% (average) and 15.3% (average) for PAHs and pesticides samples, respectively (the coverage factor k = 2, the 95% confidence level). Ultimately, the method was successfully applied to determine pesticide residues in commercial samples of fruit, vegetables and grain, and soil samples for PAHs, which were collected from selected places in the Podkarpacie region. A total of 38 real samples were tested, in which 10 pesticides and 13 PAHs were determined. Proposed changes allow us to shorten the sample preparation time (by 20%) and to reduce the consumption of organic solvents (by 17%). The use of florisil for sample cleanup, instead of GCB, improves the recovery of compounds with flat particles.
3D Printing of Monolithic Proteinaceous Cantilevers Using Regenerated Silk Fibroin
Authors: Mu Xuan , Gonzalez-Obeso Constancio , Xia Zhiyu , Sahoo Jugal Kishore , Li Gang , Cebe Peggy , Zhang Yu Shrike , Kaplan David L.
Published: March 2022

Silk fibroin, regenerated from Bombyx mori, has shown considerable promise as a printable, aqueous-based ink using a bioinspired salt-bath system in our previous work. Here, we further developed and characterized silk fibroin inks that exhibit concentration-dependent fluorescence spectra at the molecular level. These insights supported extrusion-based 3D printing using concentrated silk fibroin solutions as printing inks. 3D monolithic proteinaceous structures with high aspect ratios were successfully printed using these approaches, including cantilevers only supported at one end. This work provides further insight and broadens the utility of 3D printing with silk fibroin inks for the microfabrication of proteinaceous structures.