bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological variability and diagnostics of two sculpins Icelus bicornis and I. spatula (Cottiformes: Cottidae) from the Arctic
Authors: Zorina A.A. , Chernova N.V.
Published: June 2022

The morphological variability of Icelus spatula Gilbert et Burke, 1912 and I. bicornis Reinhardt, 1840 was studied in order to clarify the diagnostic characters of these poorly studied fishes. Allometry, geographic variability and sexual dimorphism of species were evaluated by statistical methods based on 24 counts and 30 morphometric indices. Samples from 5 Arctic regions were studied: southwestern Greenland, Spitsbergen, Barents Sea, Laptev and East Siberian seas. An analysis of the variability of diagnostic characters showed that the following can be used to identify species with high certainty: 1) the shape of the urogenital papilla (males); 2) the presence/absence of spinules on the lower-posterior margin of scales of the lateral line below the pore of the canal and the degree of development of spinules on the dorsal margin of these scales; 3) the presence of scales at the base of the caudal fin; 4) presence/absence of bony granules on the skin between the lateral line and dorsal row of scales. Other characters (the number of scales in the lateral line and in rows on the body behind the base of the pectoral fin, the length of the nuchal spines) are more variable. No clinal changes in meristic characters were found over the studied areas of ranges of the two species. Greenland population of I. bicornis differs markedly from the samples from the Barents and Laptev seas, which suggests the possibility of the existence of groups of subspecies rank in these waters. An updated key for identification of juveniles, females and males of I. bicornis and I. spatula is provided.
Evaluation of additive interaction in concrete
Authors: Endzhievskaya I. G. , Demina A. V. , Endzhievskii A. S. , Dubrovskaya S. D.
Published: June 2022

In modern construction, mineral additives are considered as important concrete components, which improve many technical parameters. Being an inert additive, finely dispersed limestone behaves as a reactive one in a system with super- and hyper-plasticizers, especially in a hardening cement, with the formation of a gas phase. The outgassing is influenced by the system rheology and can be significantly increased with deceasing viscosity, for example, when working with self-leveling mixtures. In this case, the micro-filling function of dispersed  additives  may  be  not  sufficiently implemented,  and  the optimum content  has  not a positive effect on the structure and properties of concrete. It is therefore advisable to evaluate the compatibility of mineral and chemical additives, which has been little studied to date.
Architecture of media objects in terms of the IT development
Authors: Bochkareva A. R. , Likhachev E. N.
Published: June 2022

With the creation of the first computer and beginning of digitalization, the emergence of new functional and planning types of media objects have significantly accelerated. Here belong architectural objects and spaces that solve the problems of communication and information exchange processes. Combined IT and new design approaches to media objects appear by the end of the 20th century. Their architecture reflects a rapid change in the society caused by technological progress, economic and political factors.

The development trends of media objects late in the 20th century facilitated the development of multifunctional complexes such as media centers, functional and planning types of which are being currently developed. Design and formation of media centers exhibit a changing cultural paradigm, which reflects the technological progress. The article discusses the impact of rapidly developing information technologies on the architecture of media objects. The main results include the diagram of the evolution of architectural types of media objects in the context of IT and the formation of foreign media centers.
Architecture of palaces of culture and club buildings in Siberia (Novosibirsk, Tomsk, Omsk)
Authors: Dukhanina E. S.
Published: June 2022

The problem of renovation of club buildings in modern Russia dates back to the 1990s. However, significant reforms in the sphere of cultural objects occurred only in 2013. In order to preserve and improve the existing system of club buildings, it is necessary to update knowledge in the field of club building construction in Siberia.

The article studies the formation of Siberian club building in the Soviet and modern theory of architecture. The studies of urban planning and regional architecture of Siberian club buildings distinguish their influence on the development and construction of Siberian cities. Other investigations include the architectural style of club buildings. The latter are regarded as Stalinist architectural monuments, constructivism and creative concept ASNOVA-ARU.
Reliability of target selection in the network of geographically separated radar stations in joint processing of radar information in the conditions of relayed interference
Authors: Levin Dmitriy , Parshutkin Andrey , Timoshenko Aleksandr
Published: June 2022

Introduction: Radar information processing methods are used to identify targets at short observation intervals, based on the identification of marks of different radar stations and the parametric identification of a target. However, a mutual comparison of the effectiveness of using such methods of target selection in the conditions of relayed interference has not been carried out. Purpose: The comparison of the reliability of target selection in the conditions of relayed interference in a network of geographically separated radar stations for space surveillance with the implementation of correlation ellipsoid method, strobe method of target selection and spatial separation of measured target positions method. Results: We give the decision rules for dividing the space of coordinate difference of a target into subdomains to make a decision about the truth of the target. We have carried out simulation modeling of the target selection in the conditions of relayed interference and have obtained the dependencies for the change in the probability of erroneous selection of false marks on the normalized range which is measured from the middle of the spacing base of the two geographically separated radar stations. There are also quantitative estimates of the reliability of target selection for various conditions of radar surveillance by two geographically separated radar stations. The gain in increasing the normalized range with the use of the correlation ellipsoid method as compared to strobe methods of target selection and spatial separation of measured target positions method ranged from 36% to 46%. It is shown that in most practical situations one can use the simplest method, that is the spatial separation of measured target positions method, and when solving most important problems the correlation ellipsoid method can be used. Practical relevance: Research results can be used in the development of target selection algorithms in the conditions of relayed interference.
Method for creating synthetic data sets for training neural network models for object recognition
Authors: Pchelintsev Sergey , Liashkov Mikhail , Kovaleva Olga
Published: June 2022

Introduction: The lack of training data leads to low accuracy of visual pattern recognition. One way to solve this problem is to use real data in combination with synthetic data. Purpose: To improve the performance of pattern recognition systems in computer vision by mixing real and synthetic data for training, and to reduce the time needed for preparing training data. Results: We have built an intelligent information system on the basis of the proposed method which allows the generation of synthetic images. The system allows to generate large and representative samples of images for pattern recognition neural network training. We have also developed software for the synthetic image generator for neural network training. The generator has a modular architecture, which makes it easy to modify, remove or add individual stages to the synthetic image generation pipeline. One can adjust individual parameters (like lighting or blurring) for generated images. The experiment was aimed to compare the accuracy of pattern recognition for a neural network trained on different training samples. The combination of real and synthetic data in model training showed the best recognition performance. Artificially generated training samples, in which the scale of background objects is approximately equal to the scale of the object of interest, and the number of objects of interest in the frame is higher, turned out to be more efficient than other artificially constructed training samples. Changing focal length of the camera in the synthetic image generation scene had no effect on the recognition performance. Practical relevance: The proposed image generation method allows to create a large set of artificially constructed data for training neural networks in pattern recognition in less time than it would take to create the same set of real data.
Особливості темпераменту в студентів філологічного та економічного факультетів
Authors: Котенко Юлія Олегівна
Published: June 2022

Ще в XVI ст.. Френсіс Бекон говорив, що щасливі ті, чия природа знаходиться в злагоді з їхніми заняттями. Це положення відобразилося і в поглядах психотехніків в 1920- х років. Наприклад один найвідоміший з них , О. Липманн зв’язував професію з типом темпераменту. Е. Клапаред також вважав , що одні професії підходять людям з живим темпераментом а іншим – особистостям зі спокійним. Хоч Гуревич і зауважив , що кожна людина може оволодіти будь – якою професією , але він при цьому наголосив, що скільки часу та сил потрібно буде – не відомо[1]. Тому ми вважаємо за доцільне прослідити і виявити особливості темпераменту, що мають прояв у сферах діяльності студентів філологічного та економічного факультетів.
Проблема керування розумовими діями студентів
Authors: Воронін Анатолій Іванович
Published: June 2022

Вивчення способів ефективного керування розумовими діями студентів є одним з найважливіших завдань педагогічної психології вищої школи, яке розв’язується у контексті професійного навчання. Справа в тому, що якість засвоєння студентами професійного теоретичного досвіду залежить від адекватності усвідомлених пізнавальних актів, ступеня розумової активності та оптимальної направленості мислення. У свою чергу все це залежить від умінь викладача ВУЗа створювати необхідні психолого-педагогічні умови функціонування мислення студентів, тобто, від умінь активізувати та спрямовувати розумові дії у різноманітних навчальних ситуаціях. Саме тому питання ефективної педагогічної корекції розумових дій звичайно розглядається при вивченні у ВУЗі курсу «Психологія вищої школи», коли мова йде про теоретичне мислення студентів, яке спрямоване на розв’язання різних задач з профілюючих дисциплін [4], [9].
Соціально-психологічні особливості студентів успішних у навчанні
Authors: Гордієнко Ірина Володимирівна
Published: June 2022

Соціальна ситуація в країні в умовах кризи особливо гостро потребує високо кваліфікованих та добре підготовлених фахівців у різних галузях. Сучасному суспільству необхідні добре освічені, різнобічно розвинені, ініціативні особистості, що здатні до творчої діяльності, можуть взяти на себе відповідальність за процес цієї діяльності та її наслідки.
Врахування специфіки майбутньої професії студентів при викладанні курсу «Загальна психологія»
Authors: Халік Олена Олександрівна
Published: June 2022

Вивчення циклу психологічних дисциплін є обов’язковим при отриманні студентами педагогічної освіти. На сучасному етапі у зв'язку із залученням вищих навчальних закладів України до Болонського процесу поставлено завдання докорінної перебудови засад вищої школи. Зміна характеру навчання у вузі означає зміну основного підходу до цього процесу загалом і до викладання кожного навчального предмету зокрема. Згідно з цим, на погляд Т. М. Зелінської, С. В. Воронової та ін. є важливою реалізація міждисциплінарного зв'язку між навчальними предметами студентів. Це дасть змогу поглибити певний освітній та кваліфікаційний рівень особистості при здобуванні тієї чи іншої педагогічної спеціальності [2].
Climate Change Vulnerability Assessment for the Major Habitats and Species in Lung Ngoc Hoang Nature Reserve, Vietnamese Mekong Delta
Authors:   College of Environment and Natural Resources, Can Tho University, Ninh Kieu District, Can Tho City 900000, Vietnam, Giao Nguyen Thanh , Loi Ly Van ,   College of Environment and Natural Resources, Can Tho University, Ninh Kieu District, Can Tho City 900000, Vietnam, Nhien Huynh Thi Hong ,   College of Environment and Natural Resources, Can Tho University, Ninh Kieu District, Can Tho City 900000, Vietnam, Huy Tran Ngoc ,   College of Environment and Natural Resources, Can Tho University, Ninh Kieu District, Can Tho City 900000, Vietnam
Published: June 2022

The study assessed the vulnerability of habitats and species to climate change in Lung Ngoc Hoang Nature Reserve (NR), Vietnam. The vulnerability assessment tools for habitat and species were developed by the International Union for Conservation of Nature (IUCN). Community members, NR managers and experts in the fields of environment, economic and rural development were involved in this study. The results showed that saltwater intrusion and inundation could cause serious threats to habitats (i.e., open water, Lung, agricultural and Melaleuca habitats) and freshwater species. The combined impacts of drought and high temperature potentially increase forest fires for the Melaleuca habitat and decrease the quantity and quality of open water habitats. The Melaleuca and Lung habitats have a high baseline conservation status, in which Melaleuca habitats are more vulnerable than Lung habitats. Conversely, open water and agricultural habitats are at low baseline conservation status, but open water habitats are more vulnerable. In addition, the proliferation of invasive alien species, encroachment on agricultural cultivation, and the degradation of water quality are also great threats to the NR. Key species, including Melaleuca cajuputi, Elaeocarpus hygrophilus, Chitala ornate, Channa micropeltes, were at low threat of climate change. However, C. ornate and C. micropeltes are seriously endangered by seawater intrusion, drought and poor water quality. The findings of this study can provide essential information for NR managers to formulate water management plans for the protection and management of the habitats and species in Lung Ngoc Hoang NR.
Analysis of Biography-Based Activities in Values Education
Authors: YARIM Mehmet Ali
Published: June 2022

This study aimed to examine the activities based on biography and local elements used in values education processes in primary schools. For this purpose, the study group of the research was selected by the purposeful sampling method. The study group of the research consists of 12 teachers working in primary schools in Erzurum in the 2020-2021 academic year. The research is a hermeneutic phenomenological study, one of the qualitative research models. In the research, data were collected through interviews and analyzed with content analysis. According to the results of the research, teachers think that the necessity and importance of values education in education and training is at a very high level. However, he thinks that the existing values education activities in schools are insufficient and should be further developed and enriched. In values education in schools, techniques such as watching videos, drama, painting, writing poems or texts, and narration are generally used. In addition to these, biographies of popular and national people and heroes and local elements and personalities are also used. Localization and biography-based techniques increase effectiveness and success in values education.
IPCC’nin Yeni Yayımlanan İklim Değişikliğinin Etkileri, Uyum ve Etkilenebilirlik Raporu Bize Neler Söylüyor?
Authors: TÜRKEŞ Murat
Published: June 2022

İklim değişikliği etkilerinin gelecek yıllarda güvenlik ve huzurumuzu artan bir biçimde yeniden şekillendireceğini iyi biliyoruz. En geniş anlamıyla var olan sosyal coğrafya (ekonomi, nüfus, enerji, sanayi, enerji coğrafyası, vb. içerir) ve fiziki coğrafya (atmosfer, hava ve iklimi, hidrolojiyi ve su kaynaklarını, jeomorfolojiyi, ekolojiyi, bitki örtüsünü, toprağı, vb. içerir) koşullarımız ve çevremiz günümüzde bir geçiş evresindedir ve toplumların gelecekteki fonksiyonlarının nasıl olacağına ilişkin önemli göstergeler sergilemektedir. Bu tüm bölgeler için ciddi sosyal, ekonomik ve politik etkiler şeklinde yansıyabilecektir. Çeşitli adaptasyon (uyum) kapasitelerine sahip olan ülkeler, etkileri farklı yollarla ele alabilirken, etkin, deneyimli, kararlı ve iklim direngen kurumları ve sosyoekonomik sektörleri olmayan birçok gelişmekte olan ülke ve kırılgan devletler (küçük ada devletleri, alçak kıyı ve kurak iklim ülkeleri, vb.), iklim değişikliğinden daha fazla etkilenmektedir. Bu durum gelecekte yüksek olasılıkla daha da kuvvetlenecektir.Hükümetlerarası İklim Değişikliği Paneli’nin (IPCC) 6. Değerlendirme Raporu kapsamında yaklaşık 4 yıl süren çalışmaların ikinci ayağı olan IPCC İkinci Çalışma Grubu’nun “İklim Değişikliği: Etkiler, Uyum ve Etkilenebilirlik” başlık yeni raporu 2022 Şubat sonunda tamamlandı. Raporun başlıca sonuçları ve mesajları Politikacılar İçin Özet Raporu aracılığıyla Dünya’ya açıklandı. Ancak raporun açıklandığı günler, Rusya-Ukrayna gerilimi ve savaşına denk geldiği için Rapor büyük ölçüde savaşın gölgesinde kaldı. Bu ilgisizlik ve olumsuzluk -hep olduğu gibi- Türkiye’deki iklim (hava, iklim şiddetli hava ve iklim olayları ve afetleri, vb.) haberciliğinin gündemi izleme ve değerlendirmedeki zayıflığı ve süreksizliği ya da rasgeleliği yüzünden Türkiye’de çok daha belirgindi, bana göre.Bu makalede, IPCC 6. Değerlendirme Raporu kapsamında İkinci Çalışma Grubu’nun hazırladığı “İklim Değişikliği: Etkiler, Uyum ve Etkilenebilirlik” başlık yeni raporunun geniş açılı ama kısa bir bilimsel derleme ve değerlendirmesini yapmayı amaçlıyorum.
Staging Maria Edgeworth's New Woman in Whim for Whim (1798)
Authors:   EOI A Coruña /University of A Coruña, Fernández Rodríguez Carmen María
Published: June 2022

Literary lioness Maria Edgeworth (1767-1848) is best known for her narratives of Irish setting and for the pedagogical volumes of children’s stories written in collaboration with her father, Richard Lovell Edgeworth. A prolific multifaceted author, Edgeworth composed some plays which, in general, have been overlooked by scholars. Nevertheless, Edgeworth was interested in the stage though drama represented a genre that cast some doubt on the reputation of a woman writer. It is precisely in one of Edgeworth’s comedies, Whim for Whim (1798), where she offers a portrait of the new woman as envisioned at the end of the eighteenth century. The play not only inspired memorable characters in Edgeworth’s canon, but also explored women’s role in the public and private sphere and dealt with women’s education and importance in the family. Palabras Clave:
Prince Hal and Hamilton: becoming a leader in Shakespeare’s Henriad and Lin-Manuel Miranda’s musical
Authors:   Universidad Nacional de Educación a Distancia, Ana Crespo Roca Ana
Published: June 2022

The purpose of this article is to explore the similarities between the musical Hamilton and the Henriad. It shows how Miranda and Shakespeare use similar strategies to depict the evolution of characters that become leaders. Prince Hal and Hamilton have to combine political and martial abilities to attain power. Hal learns from his rival Hotspur to be braver in battle, while Hamilton learns from Burr to be more cautious. However, only Hal is successful in keeping himself in power by balancing both types of skills. Another parallelism shared by the plays is how they show the negative consequences of the actions of the leaders by incorporating the voices of other characters. A secondary objective of this piece of writing is to explore Shakespearean elements in contemporary pop culture. In that regard, this study contests the traditional negative view of pop culture that considers popular manifestations of Shakespeare as lesser versions of the originals.
НАРАТИВНИЙ ГРИФОНАЖ ЯК ТЕХНІКА ТВОРЕННЯ ТЕКСТОВО-ГРАФІЧНИХ НАРАТИВІВ
Authors: ЦАПІВ Алла
Published: June 2022

Наукову розвідку присвячено дослідженню особливостей конструювання графічних наративів, адресатом яких є читач-дитина. Теоретико-методологічне підґрунтя дослідження становлять праці з класичної та мультимодальної наратології, а також наукові доробки, сфокусовані на вивчення художніх текстів для дітей. Під графічним наративом розуміємо художній текст, створений за допомогою вербальних та невербальних засобів, у якому відбито історію про подію, що розгортається у художньому часі і просторі, і оповідається наратором крізь призму фокалізатора-дитини. До вербальних засобів творення історії відносимо текст, а до невербальних засобів належать малюнки, типографіка, використання різного кеглю та типу шрифту літер, гра з простором сторінки, стрілки, позначки тощо. Матеріалом дослідження послугували графічні наративи “Diary of a Wimpy Kid”, створені американським письменником та розробником комп’ютерних ігор Джефом Кінні та “Tom Gates. Biscuits, Bands and very big Plans”, авторкою якого є англійська письменниця Ліз Пішон. Графічні наративи створено у формі щоденника, який ведуть підлітки на ім’я Грег та Том. Особливістю графічного наративу є саме техніка його створення, яку ми кваліфікуємо як наративний грифонаж. Наративний грифонаж – техніка вербального та невербального творення історії, однією з особливостей якої є візуальний вигляд наративу, що нагадує побіжні ескізи/замальовки дитини, а шрифт літер імітує рукописний почерк дитини (підлітка). Наратором та фокалізатором досліджуваних наративів є Грег/Том, а графічні позначки, малюнки, простір сторінки стають графічними засобами творення його точки зору як фокалізатора. У роботі виявлено графічні засоби творення візуальної образності, як-от візуальної метонімії. Визначено роль малюнків у творенні смислів загалом та коннструюванні наративу зокрема.
ТЕОРЕТИЧНІ ПЕРЕДУМОВИ СТВОРЕННЯ ТА УМОВИ ВИКОРИСТАННЯ МОДУЛЬНОЇ ТЕХНОЛОГІЇ ІНШОМОВНОЇ ПІДГОТОВКИ СТУДЕНТІВ ТЕХНІЧНОГО ВУЗУ
Authors: ЩИПАЧОВА Дар’я
Published: June 2022

У сучасних умовах розширення міжнародних зв’язків, науково-технічного співробітництва, можливостей стажування за кордоном, роботи з технічною документацією іноземною мовою потрібен високий рівень іншомовної підготовки студентів, які навчаються у технічних ВНЗ. Іншомовна комунікативна компетентність стає невід’ємною частиною загальної професійної компетентності студентів технічного ВНЗ та надає можливість майбутнім випускникам виходити на міжнародний рівень спілкування з фахівцями з інших країн. Існують різні технології, що використовуються при іншомовній підготовці студентів, однак особливої уваги заслуговує модульна технологія, що дозволяє представити все навчання у вигляді сукупності окремих змістовних блоків із конкретними компетенціями, організаційними формами. Мета статті є огляд та аналіз теоретичних передумов створення та умов використання модульної технології іншомовної підготовки студентів технічного ВНЗ. Методологічну та загально теоретичну основу дослідження склали вітчизняні та зарубіжні теорії, концепції, що відображають сучасні педагогічні та психологічні теорії професійної освіти, наукові праці вітчизняних та зарубіжних авторів щодо навчання іноземних мов, проблеми іншомовної підготовки студентів вищих навчальних закладів, в яких розкриваються: ідеї філософії, професійної діяльності, основи психологічної теорії діяльності та методологічні підходи до організації освітнього процесу. Теоретична значущість статті полягає в наступному: уточнено поняття «іншомовна комунікативна компетентність» з погляду її структури та змісту; обґрунтовано, що модель модульної технології іншомовної підготовки студентів технічного вузу розвиває ідеї компетентнісно-орієнтованої освіти, дозволяє проектувати цілі, зміст, технології, що забезпечують якісно нову іншомовну підготовку, адекватну сучасним вимогам суспільства. Вищевикладене дозволяє зробити висновок про те, що технологія модульного навчання – це не просто педагогічна технологія, що застосовується при навчанні студентів іноземної мови, а необхідна умова оволодіння іншомовною культурою через створення сприятливих умов для розвитку майбутніх інженерів як культурно-мовних осіб, які володіють високорозвиненими комунікативно-культурними вміннями під час вирішення комунікативних завдань.
RISK MANAGEMENT PRACTICES OF ISLAMIC AND CONVENTIONAL BANKS OF PAKISTAN: A COMPARATIVE STUDY
Authors: Bashir Rubina , Azeez A. A.
Published: June 2022

This paper is the report of a study that has investigated the impact of the risk management process, including liquidity and operational risk, on the risk management practices of Islamic banks and conventional banks in Pakistan. The study compared the risk management practices of both types of banks. Data was collected through the 200 self- administered questionnaires distributed to senior managers and risk officials of both types of banks. The data was analyzed using basic descriptive statistics, One-way ANOVA and Multiple regression analysis. The empirical results showed that risk assessment and analysis, risk monitoring and liquidity risk analysis were the most influential determinants of the risk management practices of conventional banks, whereas risk identification, risk assessment and analysis, credit risk analysis, liquidity risk analysis and operational risk analysis had influenced the risk management practices of Islamic banks. Overall, Islamic banks were better in terms of understanding risk management, risk identification, risk assessment and analysis, risk monitoring and operational risk analysis, while conventional banks were ahead of Islamic banks in credit risk analysis, liquidity risk analysis, and risk management practices. In the context of the emerging economy of Pakistan, the study outcomes can be helpful for investors, potential and existing customers to make prudent investment decisions. Findings are also helpful for strategy managers and regulators in policy formulation, elevation, and implementation of risk management regulations.
Evolução do perfil motor de autistas após intervenção psicomotora breve
Authors: Alves Larissa Mirela da Silveira , Santos Nilce Maria de Freitas , Castro Gisélia Gonçalves de
Published: June 2022

Introdução: O Transtorno do Espectro Autista (TEA) é um distúrbio de desenvolvimento neurológico que pressupõe estar presente desde a infância, apresentando comprometimentos sociocomunicativos e comportamentais. Objetivo: Verificar a evolução do perfil motor de autistas. Métodos: Estudo transversal intervencionista e quantitativo realizado no Centro de Reabilitação Infantil em um município mineiro. A amostra foi composta por 14 crianças com diagnóstico de TEA, com idade entre 2 e 10 anos, ambos os gêneros. Aplicou-se, inicialmente, instrumento de coleta de dados com os responsáveis pela criança. A escala de desenvolvimento motor de Rosa Neto (EDM) foi aplicada antes e após a intervenção. Os dados foram analisados através do Excel. Resultados: Referindo-se à motricidade fina, 42,86% das crianças obtiveram melhora. Quanto a motricidade global, 50% mostraram resultados satisfatórios. A maioria da amostra obteve bons resultados em relação ao equilíbrio (71,43), ao esquema corporal (64,28%) e à organização espacial (64,28%). Referindo-se à organização temporal, 42,86% crianças obtiveram resultados significativos. Apenas 14,28% mantiveram os resultados obtidos anterior à intervenção, não ocorrendo melhora no desenvolvimento psicomotor. Conclusão: Houve melhora no perfil psicomotor da maioria das crianças com TEA em todas as variáveis da escala de EDM após período de intervenção psicomotora breve.
In vitro Propagation and Genetic Homogeneity Assessment of Dendrobium crepidatum Lindley & Paxton
Authors: Dhungana Savita , Pradhan Shreeti , Paudel Mukti Ram , Pant Bijaya
Published: June 2022

In vitro propagation is one of the most dependable methods to develop true-to-type plants for the commercial and conservation aspects. This study aims to produce the genetically identical plantlets of Dendrobium crepidatum Lindley and Paxton using in vitrodeveloped shoot explants. Coconut water (5 - 10%) supplemented Murashige and Skoog (MS) medium responded towards effective shoot formation with BAP (1.0 - 2.0 mg/l) and NAA (0.1 - 0.5 mg/l). NAA (0.5 - 1.0 mg/l) alone or in combination with BAP was found to be effective for root initiation in MS medium. Four RAPD (Random Amplified Polymorphic DNA) primers viz., G04, G10, G17 and G18 showed 100% genetic homogeneity in plants obtained from in vitro-developed shoot explant. However, G10, G17 and G18 RAPD primers showed 60, 25 and 100% genetic polymorphisms respectively in plants obtained from callus. Plants obtained from callus were genetically polymorphic where somaclonal variation might occur. This study reported the successful genetic homogeneity of shoot explant-derived plants of D. crepidatum. Hence, the present study has validated the establishment of standard in vitro protocol for significant propagation of superior genetically indistinguishable D. crepidatum for viable conservation and commercialization.
Plant Tissue Cult. & Biotech. 32(1): 1-11, 2022 (June)
In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis
Authors: Pyati Ashok N
Published: June 2022

An efficient in vitro regeneration protocol through somatic embryogenesis was established from longitudinally bisected protocorm (lTCL) of an endangered orchid Dendrobium ovatum. The efficiency of EC and SEs from the protocorms significantly relied on the concentration of PGRs. MS medium supplemented with TDZ (1.0 mg/l) induced optimum of EC (31.8%) and SEs (28.1/explant). Similarly, ZEA (0.5 mg/l) induced EC (27.6%) and SEs (17.1/explant). The combined treatment of TDZ (1.0 mg/l) and NAA (0.5 mg/l) resulted in the maximum induction of EC (58.6%) and SEs (39.8/explant) in an upright incubated explant. In another combined effect of ZEA (1.0 mg/l) and NAA (0.5 mg/l) induced EC (43.8%) and SEs (24.3/explant), whereas the combination of BAP (0.5 mg/l) and NAA (0.5 mg/l) produced EC (34.4%) and SEs (16.8/explants). The induced EC and SEs where healthier on medium containing TDZ + NAA than on the medium containing ZEA+NAA and BAP+NAA. The orientation of lTCL explants seemed to interact with position to affect the morphogenesis. The lTCL explants incubated in the upright orientation induced higher percentage of EC and a greater number of SEs/explants (EC-58.6% and SEs-39.8/explants) than that in the inverted orientation (EC- 16.4% and SEs-9.1/explants) irrespective of the PGRs. The SEs developed from the EC and the intermediate stage of PLBs were finally differentiated into plantlets. About 89% of plantlets were successfully acclimatized in the green house conditions.
Plant Tissue Cult. & Biotech. 32(1): 53-66, 2022 (June)
In vitro Regeneration of Strawberry Plant from Leaf Explants via Callus Induction
Authors: Yeasmin Sahida , Banu Tanjina Akhtar , Goswami Barna , Hasan Sarkar Md Murshed , Jahan Iffat , Habib Ahashan , Khan Salim , Akter Shahina
Published: June 2022

The present investigation was carried out to develop an efficient in vitro regeneration protocol for strawberry plant using leaf explants. The highest percentage of callus induction was obtained on MS medium supplemented with 2.0 mg/l BAP and 0.5mg/l IBA. The highest numbers of shoots were regenerated from the callus on MS medium supplemented with 2.0 mg/l BAP and 1.5mg/l IBA. In case of root induction, MS medium supplemented with 1 mg/l IBA was found to be most effective. Following the development of roots, the in vitro regenerated plantlets were successfully transplanted into soil.
Plant Tissue Cult. & Biotech. 32(1): 67-75, 2022 (June)
Australian vaccine preventable disease epidemiological review series: measles, 2012–2019
Authors: Winkler Noni E , Dey Aditi , Quinn Helen E , Pourmarzi Davoud , Lambert Stephen , McIntyre Peter , Beard Frank
Published: June 2022

Background Data sources, relevant to measles epidemiology from 2012 to 2019, were reviewed in the context of Australia’s certification, by the World Health Organization in 2014, of the elimination of measles. Methods Data on measles notifications, hospitalisations, and deaths were obtained from the National Notifiable Diseases Surveillance System, the National Hospital Morbidity Database, and the Australian Coordinating Registry. Data were analysed by age group, state/territory, Aboriginal and Torres Strait Islander status, genotype, place of acquisition, source of infection (importation status), and vaccination status. Results Between 2012 and 2019, there were 1,337 measles notifications (average annual notifications 0.7 per 100,000 population per year) and 425 hospitalisations with measles as principal diagnosis (0.3 per 100,000 population per year) were recorded. The highest annual notification rate was in 2014, when the rate in the Northern Territory was 21.4 per 100,000 population per year. Although notification and hospitalisation rates were highest in infants < 12 months (respectively 5.8 and 2.1 per 100,000 population per year), people aged 10 to 39 years (10–19y: 272 notifications; 20–29y: 347; 30–39y: 266) accounted for 66% of notified cases. Of cases with a known vaccination status, only 20/169 (11.8%) of those aged 1–9 years had received at least one dose of measles-containing vaccine, compared with 215/571 (37.7%) of those aged 10–39 years. Persons born before 1966 (at least 47 years of age during the study period) are likely to have immunity from wild-type measles infection and had the lowest notification rates in each year. Of notified cases, 98.1% were imported or import related, and of the 900 measles viruses genotyped, D8 and B3 accounted for 89.1%. Conclusion This review’s findings of low measles incidence, in the presence of robust surveillance and high two-dose measles vaccination coverage, provide evidence of continued elimination of endemic measles in Australia, with almost all cases imported or epidemiologically linked to an imported case. Most cases eligible for vaccination are unvaccinated, which should remain the primary focus for prevention. Potential waning immunity in older age groups requires monitoring. Continued high population immunity and high-quality public health response to cases will be needed to maintain Australia’s elimination status, particularly once international borders reopen.
Characterization of Extracellular vesicles isolated from different Liquid biopsies of uveal melanoma patients
Authors: Luz Pessuti Carmen , Fialho Costa Deise , Ribeiro Kleber S. , Abdouh Mohamed , Tsering Thupten , Nascimento Heloisa , Commodaro Alessandra G. , Affonso Antunes Marcos Allexya , Torrecilhas Ana Claudia , Belfort Rubens N. , Belfort Jr Rubens , Valdemarin Burnier Julia
Published: June 2022

Purpose: Uveal melanoma (UM) is the most common intraocular malignant tumor in adults. Extracellular vesicles (EVs) have been extensively studied as a biomarker to monitor disease in patients. The study of new biomarkers in melanoma patients could prevent metastasis by earlier diagnosis. In this study, we determined the proteomic profile of EVs isolated from aqueous humor (AH), vitreous humor (VH), and plasma from UM patients in comparison with cancer-free control patients.
Methods: AH, VH and plasma were collected from seven patients with UM after enucleation; AH and plasma were collected from seven cancer-free patients with cataract (CAT; control group). EVs were isolated using the membrane-based affinity binding column method. Nanoparticle tracking analysis (NTA) was performed to determine the size and concentration of EVs. EV markers, CD63 and TSG101, were assessed by immunoblotting, and the EV proteome was characterized by mass spectrometry.
Results: Mean EV concentration was higher in all analytes of UM patients compared to those in the CAT group. In the UM cohort, the mean concentration of EVs was significantly lower in AH and plasma than in VH. In contrast, the mean size and size distribution of EVs was invariably identical in all analyzed analytes and in both studied groups (UM vs. CAT). Mass spectrometry analyses from the different analytes from UM patients showed the presence of EV markers.
Conclusion: EVs isolated from AH, VH, and plasma from patients with UM showed consistent profiles and support the use of blood to monitor UM patients as a noninvasive liquid biopsy.
The Effectiveness of the Entrepreneurship MBKM Lecture Model: Alternatives to Improve Student Creativity Competencies during the Pandemic Covid-19
Authors: Dwikoranto Dwikoranto
Published: June 2022

This study aims to produce an effective Entrepreneurship MBKM model in increasing student creativity by producing lecture outputs by students in the form of business plan drafts, startups, and entrepreneurship PkM proposals. The MBKM Entrepreneurship model used is said to be effective if there is an increase in creativity competence and it produces lecture outputs by students which are statistically significant and the average n-gain is at least in the moderate category. The research design in this study used a pre-experiment with a one group pre-test and post-test design. The research subjects were 30 students of Unesa physics in the 2021/2022 academic year. The instrument used in this research is a creativity assessment to assess student portfolio results. Data were analyzed using paired t-test and N-gain to see the increase in creativity. The results showed that there was a difference in creativity before and after the model was applied and a significant increase in student creativity and the average N-gain increase in student creativity in the medium criteria. In addition, students are able to produce creative products in the form of business plan drafts, startups, and entrepreneurship PkM proposals with a minimum score of B+.
SIFAT SIRALAMALARI ÜZERİNDE YENİ BİR KISITLAMA
Authors: BAYIRLI İsa Kerem
Published: June 2022

Bu yazıda sıfat sıralamaları ile ilgili yeni bir genelleme ortaya atılmakta ve bu genellemenin Son-üstü-Son Kısıtı tarafından açıklanabileği gösterilmektedir.

Yazıda ilk olarak Son-üstü-Son Kısıtı betimlenmekte ve bu kısıtın açıklayıcı gücüne örnekler verilmektedir. Daha sonra sıfatlar ile ilgili bazı gözlemler ışığında sıfatların dizimi ile ilgili iki analiz sunulmaktadır: (a) bitiştirme analizi ve (b) işlevsel baş analizi. Yazının sonraki bölümünde sıfat sıralamaları üzerinde adına Sıfat Sırası Genellemesi denilen bir kısıtlamanın var olduğu savunulmaktadır. Son olarak sıfatların işlevsel baş analizi varsayıldığında Sıfat Sırası Genellemesinin Son-üstü-Son Kısıtının sonuçlarından biri olarak ele alınabileceği gösterilmektedir. Yazı, Son-üstü-Son Kısıtının neden geçerli olduğunu açıklayan dizim-temelli bir analizin betimlenmesiyle sona ermektedir.
Reducing the Pain of Infants due to Vaccine Injection: A Randomized Controlled Trial
Authors: ATEŞ BEŞİRİK Selda , GÖZEN Duygu
Published: June 2022

Objective: This study was conducted as a randomized controlled and experimental to compare the effect of breastfeeding and distraction methods on vital signs, pain level, and the duration of crying due to vaccine injection in healthy infants.

Methods: The population of the study consisted of 120 infants between 1 and 12 months of age who had met the inclusion criteria. The sample group was randomized and divided into two groups. The control group was breastfeed according to clinical procedure, including 58 infants, and the distraction group was include 62 infants. For both groups, the oxygen saturation (SpO2), heart rate (HR), crying duration, and pain scores were compared both before and following vaccination sessions.

Results: The vaccination pain scores of the infants from the distraction group (4.39±2.18) were significantly lower than those of the breastfeeding group (7.05±1.55; p=0.001). The post-vaccination SpO2 was higher in infants in distraction group; whereas, HR was lower in this group compared to the breastfeeding group. The post-vaccination crying durations of those in the distraction group were also shorter than those in the breastfeeding group.

Conclusion: The distraction method is adopted by the use of a toy has been found to be effective in decreasing the acute pain during vaccine administrations.
In vitro Cartilage Regeneration Regulated by a Hydrostatic Pressure Bioreactor Based on Hybrid Photocrosslinkable Hydrogels
Authors: Zhao Xintong , Hua Yujie , Wang Tao , Ci Zheng , Zhang Yixin , Wang Xiaoyun , Lin Qiuning , Zhu Linyong , Zhou Guangdong
Published: June 2022

Because of the superior characteristics of photocrosslinkable hydrogels suitable for 3D cell-laden bioprinting, tissue regeneration based on photocrosslinkable hydrogels has become an important research topic. However, due to nutrient permeation obstacles caused by the dense networks and static culture conditions, there have been no successful reports on in vitro cartilage regeneration with certain thicknesses based on photocrosslinkable hydrogels. To solve this problem, hydrostatic pressure (HP) provided by the bioreactor was used to regulate the in vitro cartilage regeneration based on hybrid photocrosslinkable (HPC) hydrogel. Chondrocyte laden HPC hydrogels (CHPC) were cultured under 5 MPa HP for 8 weeks and evaluated by various staining and quantitative methods. Results demonstrated that CHPC can maintain the characteristics of HPC hydrogels and is suitable for 3D cell-laden bioprinting. However, HPC hydrogels with concentrations over 3% wt% significantly influenced cell viability and in vitro cartilage regeneration due to nutrient permeation obstacles. Fortunately, HP completely reversed the negative influences of HPC hydrogels at 3% wt%, significantly enhanced cell viability, proliferation, and extracellular matrix (ECM) deposition by improving nutrient transportation and up-regulating the expression of cartilage-specific genes, and successfully regenerated homogeneous cartilage with a thickness over 3 mm. The transcriptome sequencing results demonstrated that HP regulated in vitro cartilage regeneration primarily by inhibiting cell senescence and apoptosis, promoting ECM synthesis, suppressing ECM catabolism, and ECM structure remodeling. Evaluation of in vivo fate indicated that in vitro regenerated cartilage in the HP group further developed after implantation and formed homogeneous and mature cartilage close to the native one, suggesting significant clinical potential. The current study outlines an efficient strategy for in vitro cartilage regeneration based on photocrosslinkable hydrogel scaffolds and its in vivo application.
Synthesis and Anticancer Activity of Rhopaladins’ Analog RPDPD Against the HeLa Human Cervical Cancer Cell Line
Authors: Chen Feng , Wang Hong-Mei , Kong Ling-Qi , Chen Qin-Hua , Ke Li-Na , Dai He-Liu , Zeng Xiao-Hua
Published: June 2022

Heterocyclic compounds were widely used in many domains; pyrrolidone is a derivative of heterocycles that can be used to synthesize anticancer drugs. A new fluorine-containing rhopaladins’ analog(E)-2-(4-bromobenzoyl)-N-(tert-butyl)-4-(4-fluoro benzylidene)-5-oxo-1-propylpyrrolidine-2-carboxamide (RPDPD for short) of 2-aroyl-4-arylidene-5-oxopyrrolidine derivative was synthesized by the one-pot synthesis method and evaluated for its anti-tumor activity in vitro via CCK8 assay and annexin V/propidium iodide (PI) staining of HeLa cells. The results exhibited that compound RPDPD has inhibited the proliferation of HeLa in a dose-dependent manner with an IC50 of 24.23 μmol/L (p < 0.05) and has low hepatotoxicity with an IC50 of 235.6 μmol/L (p < 0.05) to normal hepatocyte LO2 cells. The apoptotic assay demonstrated that compound RPDPD has induced apoptosis in HeLa cells (from 14.26 to 23.4%, p < 0.05). qRT-PCR results showed that the compound RPDPD could inhibit the expression of oncogene E6/E7 mRNA (p < 0.05) of human papillomavirus (HPV). The results of Western blot showed that the compound RPDPD promoted the expression of TIMP3 protein and inhibited the expression of MMP3 (p < 0.05). In conclusion, the compound RPDPD can inhibit the proliferation of cervical cancer cells and induce the apoptosis of cervical cancer cells, and its mechanism may be related to the inhibition of E6 mRNA and E7 mRNA expressions, and the anticancer effect of the compound RPDPD on cervical cancer is closely related to the TIMP3/MMP3 signaling axis.
Nomogram Model for Prediction of SARS-CoV-2 Breakthrough Infection in Fujian: A Case–Control Real-World Study
Authors: Chen Tianbin , Zeng Yongbin , Yang Di , Ye Wenjing , Zhang Jiawei , Lin Caorui , Huang Yihao , Ye Yucheng , Li Jianwen , Ou Qishui , Li Jinming , Liu Can
Published: June 2022

SARS-CoV-2 breakthrough infections have been reported because of the reduced efficacy of vaccines against the emerging variants globally. However, an accurate model to predict SARS-CoV-2 breakthrough infection is still lacking. In this retrospective study, 6,189 vaccinated individuals, consisting of SARS-CoV-2 test-positive cases (n = 219) and test-negative controls (n = 5970) during the outbreak of the Delta variant in September 2021 in Xiamen and Putian cities, Fujian province of China, were included. The vaccinated individuals were randomly split into a training (70%) cohort and a validation (30%) cohort. In the training cohort, a visualized nomogram was built based on the stepwise multivariate logistic regression. The area under the curve (AUC) of the nomogram in the training and validation cohorts was 0.819 (95% CI, 0.780–0.858) and 0.838 (95% CI, 0.778–0.897). The calibration curves for the probability of SARS-CoV-2 breakthrough infection showed optimal agreement between prediction by nomogram and actual observation. Decision curves indicated that nomogram conferred high clinical net benefit. In conclusion, a nomogram model for predicting SARS-CoV-2 breakthrough infection based on the real-world setting was successfully constructed, which will be helpful in the management of SARS-CoV-2 breakthrough infection.