bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Salvia officinalis) under the Effect of Hydrazine Hydrate
Authors: Ali Mohammed , Abdelkawy Aisha M. , Darwish Doaa Bahaa Eldin , Alatawi Hanan Ali , Alshehri Dikhnah , Al-Amrah Hadba , Soudy Fathia A.
Published: June 2023

Mutagenesis is a highly efficient tool for establishing genetic variation and is widely used for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of enhancing one or several characteristics of a variety without altering the genetic background. In this study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ), i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and 62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore, we used the qRT-PCR system to disclose the “transcriptional control” for twelve TPS genes related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS, SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to ensure some positive relationship between the concentrations of the chemical mutagen HZ used for treating the seeds, the type and amount of the produced terpenes, and the expression of their corresponding genes.
Optofluidic Tweezers: Efficient and Versatile Micro/Nano-Manipulation Tools
Authors: Zhu Yuchen , You Minmin , Shi Yuzhi , Huang Haiyang , Wei Zeyong , He Tao , Xiong Sha , Wang Zhanshan , Cheng Xinbin
Published: June 2023

Optical tweezers (OTs) can transfer light momentum to particles, achieving the precise manipulation of particles through optical forces. Due to the properties of non-contact and precise control, OTs have provided a gateway for exploring the mysteries behind nonlinear optics, soft-condensed-matter physics, molecular biology, and analytical chemistry. In recent years, OTs have been combined with microfluidic chips to overcome their limitations in, for instance, speed and efficiency, creating a technology known as “optofluidic tweezers.” This paper describes static OTs briefly first. Next, we overview recent developments in optofluidic tweezers, summarizing advancements in capture, manipulation, sorting, and measurement based on different technologies. The focus is on various kinds of optofluidic tweezers, such as holographic optical tweezers, photonic-crystal optical tweezers, and waveguide optical tweezers. Moreover, there is a continuing trend of combining optofluidic tweezers with other techniques to achieve greater functionality, such as antigen–antibody interactions and Raman tweezers. We conclude by summarizing the main challenges and future directions in this research field.
Self-Powered, Non-Toxic, Recyclable Thermogalvanic Hydrogel Sensor for Temperature Monitoring of Edibles
Authors: Yang Kun , Bai Chenhui , Liu Boyuan , Liu Zhoutong , Cui Xiaojing
Published: June 2023

Thermogalvanic hydrogel, an environmentally friendly power source, enable the conversion of low-grade thermal energy to electrical energy and powers microelectronic devices in a variety of scenarios without the need for additional batteries. Its toxicity, mechanical fragility and low output performance are a hindrance to its wide application. Here, we demonstrate thermoelectric gels with safe non-toxic, recyclable, highly transparent and flexible stretchable properties by introducing gelatin as a polymer network and SO3/42− as a redox electric pair. When the temperature difference is 10 K, the gel-based thermogalvanic cell achieves an open-circuit voltage of about 16.2 mV with a maximum short-circuit current of 39 μA. Furthermore, we extended the application of the Gel-SO3/42− gel to monitor the temperature of hot or cold food, enabling self-powered sensing for food temperature detection. This research provides a novel concept for harvesting low-grade thermal energy and achieving safe and harmless self-driven temperature monitoring.
Study of a First Approach to the Controlled Fermentation for Lambic Beer Production
Authors: Postigo Vanesa , García Margarita , Arroyo Teresa
Published: June 2023

Non-Saccharomyces yeasts represent a great source of biodiversity for the production of new beer styles, since they can be used in different industrial areas, as pure culture starters, in co-fermentation with Saccharomyces, and in spontaneous fermentation (lambic and gueuze production, with the main contribution of Brettanomyces yeast). The fermentation process of lambic beer is characterized by different phases with a characteristic predominance of different microorganisms in each of them. As it is a spontaneous process, fermentation usually lasts from 10 months to 3 years. In this work, an attempt was made to perform a fermentation similar to the one that occurred in this process with lactic bacteria, Saccharomyces yeast and Brettanomyces yeast, but controlling their inoculation and therefore decreasing the time necessary for their action. For this purpose, after the first screening in 100 mL where eight Brettanomyces yeast strains from D.O. “Ribeira Sacra” (Galicia) were tested, one Brettanomyces bruxellensis strain was finally selected (B6) for fermentation in 1 L together with commercial strains of Saccharomyces cerevisiae S-04 yeast and Lactobacillus brevis lactic acid bacteria in different sequences. The combinations that showed the best fermentative capacity were tested in 14 L. Volatile compounds, lactic acid, acetic acid, colour, bitterness, residual sugars, ethanol, melatonin and antioxidant capacity were analysed at different maturation times of 1, 2, 6 and 12 months. Beers inoculated with Brettanomyces yeast independently of the other microorganisms showed pronounced aromas characteristic of the Brettanomyces yeast. Maturation after 12 months showed balanced beers with “Brett” aromas, as well as an increase in the antioxidant capacity of the beers.
Effects of Steel Slag Powder as A Cementitious Material on Compressive Strength of Cement-Based Composite
Authors: Sheng Guohua , Li Chao , Jin Shengji , Bai Quan
Published: June 2023

The utilization rate of steel slag in China is far behind that of developed countries. The annual output of steel slag is still increasing, resulting in a large amount of accumulation, causing environmental pollution. This paper summarizes and analyzes the relevant research on steel slag powder (SSP) as a cementitious material, studies the effect of SSP replaces cement as single or multiple admixtures, with different specific surface areas, and the amount of activator on the compressive strength of cement-based material. The results show that due to the lower content of active substances in SSP compared to cement, the strengths decrease with the increase of the replacement ratio R, which is the ratio of SSP to cement. R = 30% is important for replacing cement with single SSP. When replacing cement with the mixture of SSP and slag/fly ash, the strengths of most groups decrease with the increase of the mix replacement ratio Rc. The decreasing trend is not obvious due to the pozzolanic effect. There is an optimal dosage for using a single activator to activate SSP. The effect of using multiple activators in combination is better than that of single one. The strength increases with the increase of the specific surface area (SSA) of SSP. However, if the SSA is too high, it will not only increase the preparation cost, but also reduce the increase in strength due to the agglomeration effect of SSP. The optimal range of specific surface area SSA is 400 m2/kg~500 m2/kg. With the increase of age t, the compressive strength increases. The effect of the curing methods on the compressive strength is hot and heat curing > standard curing > natural curing.
Antigorite Dehydration under Compression and Shear Loadings in a Rotational Diamond Anvil Cell
Authors: Tan Dayong , Jiang Changguo , Chen Weishan , Tan Yi , Yue Binbin , Xiao Wansheng
Published: June 2023

Mineral dehydration in the subduction zone enormously affects Earth’s geodynamics and the global geochemical cycles of elements. This work uses Raman spectroscopy and X-ray diffraction to investigate the dehydration process of antigorite under compression and shear loading conditions in a rotational diamond anvil cell (RDAC) at room temperature. In order to compare the shear effects, T301 stainless steel and Kapton plastic are applied as the gasket materials. In the experiment using a high-strength T301 stainless steel gasket, two new broad OH-stretching peaks of H2O and H3O2− appear at 3303 and 3558 cm−1, respectively, at 1.7 GPa. The original sharp OH-stretching peaks of antigorite at 3668 and 3699 cm−1 remain, while the central pressure is increased to 8.0 GPa, and the largest pressure gradient is about 2.5 GPa in the sample chamber. In another experiment with a low-strength gasket of Kapton plastic, two new OH-stretching broad peaks of H2O and H3O2− also start to appear at 3303 and 3558 cm−1, respectively, at a lower pressure of 0.3 GPa, but the original sharp OH-stretching peaks of antigorite at 3668 and 3699 cm−1 almost completely vanish as the central pressure reaches 3.0 GPa, with the largest pressure gradient at around 4.8 GPa. The comparison between the two experiments shows that antigorite is easier to dehydrate in the chamber of a Kapton plastic gasket with a larger gradient of shear stress. However, its axial compression stress is lower. The high-pressure Raman spectra of MgO2(OH)4 octahedron and SiO4 tetrahedron in the low wavenumber zones (100–1200 cm−1) combined with the micro-beam X-ray diffraction spectrum of the recovered product strongly support the structural breakdown of antigorite. This investigation reveals that the water-bearing silicate minerals have strong shear dehydration in the cold subduction zone of the plate, which has important applications in predicting the physical and chemical properties of subduction zones and deducing the rate of plate subduction.
Investigation of Hexylamine Adsorption on Gold in Perchloric Acid
Authors: Ostapenko Gennady I. , Kalashnikova Nina A.
Published: June 2023

The adsorption of hexylamine at the solution–gold interface in 1 M HClO4 in the presence of 0.1 M Fe2+ and 0.1 Fe3+ was studied by potentiodynamic, chronoamperometric and EIS methods. The main kinetic characteristics of the oxidation-reduction reaction iron ions (exchange current density, transfer coefficient, diffusion coefficients of iron ions) were determined. It was shown that the physical adsorption of hexylamine on gold can be described by the Dhar–Flory–Huggins isotherm. The values of the adsorption constant and the Gibbs free adsorption energy were obtained. A comparison of the free adsorption energy at these interfaces with the interaction energies of hexylamine and water molecules, and hexylamine molecules with each other was carried out. It was shown that hexylamine adsorption at all of these interfaces is due mainly to the hydrophobic effect of the interaction of hexylamine and water molecules.
Modeling and Comparative Analysis of Atmospheric Pressure Anodic Carbon Arc Discharge in Argon and Helium–Producing Carbon Nanostructures
Authors: Saifutdinov Almaz , Timerkaev Boris
Published: June 2023

In this work, within the framework of a unified model for the discharge gap and electrodes, a comparative numerical analysis was carried out on the effect of evaporation of graphite anode material on the characteristics of the arc discharge in helium and argon. The effect of changing the plasma-forming ion, in which the ion of evaporated atomic carbon becomes the dominant ion, is demonstrated. For an arc discharge in helium, this effect is accompanied by a jump-like change in the dependence of the current density on voltage (CVC), and smoothly for a discharge in argon. With regard to the dynamics of the ignition of an arc discharge, it is shown that during the transition from glow discharge to arc in helium, the discharge parameters are also accompanied by an abrupt change, while in argon, this transition is smooth. This is due to the fact that the ionization potentials, as well as the ionization cross sections, differ significantly for helium and carbon, and are close in value for helium and argon. For various points on the CVC, the density distributions of the charged and neutral particles of an inert gas and evaporated gases are presented.
1337 nm Emission of a Nd3+-Doped TZA Glass Random Laser
Authors: Dipold Jessica , Bordon Camila D. S. , Magalhães Evellyn S. , Kassab Luciana R. P. , Jimenez-Villar Ernesto , Wetter Niklaus U.
Published: June 2023

Random lasers have been studied using many materials, but only a couple have used glass matrices. Here, we present a study of zinc tellurite and aluminum oxide doped with different percentages of neodymium oxide (4 wt.%, 8 wt.%, and 16 wt.%) and demonstrate for the first time random laser action at 1337 nm. Laser emission was verified and the laser pulse’s rise time and input–output power slope were obtained. A cavity composed of the sample’s pump surface and an effective mirror formed by a second, parallel layer at the gain-loss boundary was probably the main lasing mechanism of this random laser system. The reason for the absence of emission at 1064 nm is thought to be a measured temperature rise in the samples’ active volume.
A Human Microglial Cell Line Expresses γ-Aminobutyric Acid (GABA) Receptors and Responds to GABA and Muscimol by Increasing Production of IL-8
Authors: Wagner Ashley , Yan Zhimin , Kulka Marianna
Published: June 2023

Gamma-aminobutyric acid (GABA) is an essential neurotransmitter and an important regulator of neuroinflammation and disease. Microglia are important immune cells in the brain that express GABA receptors (GABAR) and respond to both GABA and GABAR agonists, yet the effect of GABA on microglial inflammatory responses is unclear. We hypothesized that GABA and GABAR agonists might modify the activation of a human microglial cell line (HMC3). We further hypothesized that Amanita muscaria extract (AME-1), which contained GABAR agonists (GABA and muscimol), would similarly stimulate HMC3. Ligand-gated GABAR (GABAAR) and G protein-coupled GABAR (GABABR) subunit expression was analyzed by qRT-PCR, metabolic activity was determined by nicotinamide adenine dinucleotide (NADH)-dependent oxidoreductase assay (XTT), reactive oxygen species (ROS) generation was analyzed by 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA), and interleukin-8 (IL-8) production was analyzed by an enzyme-linked immunosorbent assay (ELISA). HMC3 expressed several neuroreceptors such as subunits of the GABAA receptor (GABAAR). HMC3 constitutively produce IL-8 and ROS. Both muscimol and GABA stimulated HMC3 to produce more IL-8 but had no effect on constitutive ROS production. GABA and muscimol altered the morphology and Iba1 localization of HMC3. GABA, but not muscimol, increased HMC3 metabolic activity. Similarly, AME-1 induced HMC3 to produce more IL-8 but not ROS and altered cell morphology and Iba1 localization. GABA induction of IL-8 was blocked by bicuculline, an antagonist of GABAAR. AME-1-induced production of IL-8 was not blocked by bicuculline, suggesting that AME-1’s effect on HMC3 was independent of GABAAR. In conclusion, these data show that GABA and GABA agonists stimulate HMC3 to increase their production of IL-8. Mixtures that contain GABA and muscimol, such as AME-1, have similar effects on HMC3 that are independent of GABAAR.
Motor Capabilities in Children with ADHD Are Improved after Brief Visuopostural Training
Authors: Caldani Simona , Acquaviva Eric , Moscoso Ana , Landman Benjamin , Michel Alexandre , Delorme Richard , Bucci Maria Pia
Published: June 2023

Children with ADHD show poor motor control. The aim of the present study was to test whether children with ADHD improved their motor performances (oculomotor as well as posture) after a short visuopostural training period. Two groups (G1 trained and G2 non-trained), each comprising 15 children with ADHD matched in IQ (intelligence quotient), sex, and age, participated in the study. Eye movements and postural sway were measured before (T1) and after (T2) 10 min of visuopostural training for the trained group and after 10 min of resting for the non-trained group. Training consisted of a visual search task performed while the child was standing on an unstable platform. At T1, oculomotor and postural abilities were statistically similar for both groups of children with ADHD (trained and non-trained). At T2, significant improvements in both oculomotor and postural capabilities were observed for the trained group but not for the non-trained group. These findings suggest that a short visuopostural training period could help children with ADHD to learn how to focus their visual attention in order to improve motor performance. Visuopostural training could allow a better integration of sensory inputs via central mechanisms, leading to improvement in both oculomotor and postural control. Further studies on a larger number of children with ADHD will be needed to confirm these findings and explore the eventual possible persistence of the training effect.
Associations between Ileal Juice Bile Acids and Colorectal Advanced Adenoma
Authors: Luu Hung , Tran Chi , Wang Renwei , Nguyen Mai , Tran Mo , Tuong Thuy , Tran Quang , Le Linh , Pham Huong , Vu Hien , Bui Nam , Ha Hien , Trinh Dung , Thomas Claire , Adams-Haduch Jennifer , Velikokhatnaya Liudmilla , Schoen Robert , Xie Guoxiang , Jia Wei , Boffetta Paolo , Clemente Jose , Yuan Jian-Min
Published: June 2023

Background: There is an urgent need to identify biomarkers for advanced adenoma, an important precursor of colorectal cancer (CRC). We aimed to determine alterations in ileal juice bile acids associated with colorectal advanced adenoma. Methods: We quantified a comprehensive panel of primary and secondary bile acids and their conjugates using an ultraperformance liquid chromatography triple-quadrupole mass spectrometric assay in ileal juice collected at colonoscopy from 46 study subjects (i.e., 14 biopsy-confirmed advanced adenomas and 32 controls free of adenoma or cancer). Using analysis of covariance (ANCOVA), we examined the differences in bile acid concentrations by disease status, adjusting for age, sex, body mass index, smoking status and type 2 diabetes. Results: The concentrations of hyodeoxycholic acid (HCA) species in ileal juice of the advanced adenoma patients (geometric mean = 4501.9 nM) were significantly higher than those of controls (geometric mean = 1292.3 nM, p = 0.001). The relative abundance of ursodeoxycholic acid (UDCA) in total bile acids was significantly reduced in cases than controls (0.73% in cases vs. 1.33% in controls; p = 0.046). No significant difference between cases and controls was observed for concentrations of total or specific primary bile acids (i.e., cholic acid (CA), chenodeoxycholic acid (CDCA) and their glycine- and taurine-conjugates) and total and specific major secondary bile acids (i.e., deoxycholic acid and lithocholic acid). Conclusions: Colorectal advanced adenoma was associated with altered bile acids in ileal juice. The HCA species may promote the development of colorectal advanced adenoma, whereas gut microbiota responsible for the conversion of CDCA to UDCA may protect against it. Our findings have important implications for the use of bile acids as biomarkers in early detection of colorectal cancer.
Weight Bias Internalization and Eating Disorder Psychopathology in Treatment-Seeking Patients with Obesity
Authors: Calugi Simona , Segattini Barbara , Cattaneo Gianmatteo , Chimini Mirko , Dalle Grave Anna , Dametti Laura , Molgora Manuela , Dalle Grave Riccardo
Published: June 2023

This study aimed to investigate the relationship between weight bias internalization and eating disorder psychopathology in treatment-seeking patients with severe obesity using a network approach. Two thousand one hundred and thirteen patients with obesity were consecutively admitted to a specialist clinical unit for obesity and were recruited from January 2016 to February 2023. Body mass index was measured, and each patient completed the Weight Bias Internalization Scale (WBSI) and the Eating Disorder Examination Interview (EDE). Network analysis showed that the most central and highly interconnected nodes in the network were related to the EDE items exposure avoidance, dissatisfaction with shape, and wanting an empty stomach. Bridge nodes were found, but the bootstrap difference test on expected bridge influence indicated non-significant centrality differences. Nevertheless, the eating disorder psychopathology and weight bias internalization network structure in patients seeking treatment for obesity indicate the prominent roles of body dissatisfaction and control of eating and weight in these psychological constructs. This finding, if replicated, could pave the way for a new understanding of the psychological mechanisms operating in patients with obesity.
Comparison of Bevacizumab and Aflibercept for Suppression of Angiogenesis in Human Retinal Microvascular Endothelial Cells
Authors: Yazdanyar Amirfarbod , Cai Charles L. , Aranda Jacob V. , Shrier Eric , Beharry Kay D.
Published: June 2023

Bevacizumab (Avastin) is a vascular endothelial growth factor (VEGF) inhibitor that is widely used for aggressive posterior retinopathy of prematurity (APROP). Its use is associated with multiple adverse effects. Aflibercept (Eylea) is a VEGFR-1 analogue that is approved for ocular use, but its efficacy for APROP is less studied. We tested the hypothesis that Eylea is as effective as Avastin for suppression of intermittent hypoxia (IH)-induced angiogenesis. Human retinal microvascular endothelial cells (HRECs) were treated with Avastin and low- or high-dose Eylea and exposed to normoxia, hyperoxia (50% O2), or neonatal IH for 24, 48, or 72 h. Cells were assessed for migration and tube formation capacities, as well as biomarkers of angiogenesis and oxidative stress. Both doses of Eylea suppressed migration and tube formation in all oxygen environments, although the effect was not as robust as Avastin. Furthermore, the lower dose of Eylea appeared to be more effective than the higher dose. Eylea induced soluble VEGFR-1 (sVEGFR-1) coincident with high IGF-I levels and decreased Notch/Jagged-1, demonstrating a functional association. Given the role of VEGFR-1 and Notch as guidance cues for vascular sprouting, these data suggest that Eylea may promote normal vascular patterning in a dose-dependent manner.
Pistacia vera Extract Potentiates the Effect of Melatonin on Human Melatonin MT1 and MT2 Receptors with Functional Selectivity
Authors: Labani Nedjma , Gbahou Florence , Noblet Marc , Masri Bernard , Broussaud Olivier , Liu Jianfeng , Jockers Ralf
Published: June 2023

Melatonin is a tryptophan derivative synthesized in plants and animals. In humans, melatonin acts on melatonin MT1 and MT2 receptors belonging to the G protein-coupled receptor (GPCR) family. Synthetic melatonin receptor agonists are prescribed for insomnia and depressive and circadian-related disorders. Here, we tested 25 commercial plant extracts, reported to have beneficial properties in sleep disorders and anxiety, using cellular assays (2─[125I]iodomelatonin binding, cAMP inhibition, ERK1/2 activation and β-arrestin2 recruitment) in mock-transfected and HEK293 cells expressing MT1 or MT2. Various melatonin receptor-dependent and -independent effects were observed. Extract 18 (Ex18) from Pistacia vera dried fruits stood out with very potent effects in melatonin receptor expressing cells. The high content of endogenous melatonin in Ex18 (5.28 ± 0.46 mg/g extract) is consistent with this observation. Ex18 contains an additional active principle that potentiates the effect of melatonin on Gi protein-dependent pathways but not on β-arrestin2 recruitment. Further active principles potentiating exogenous melatonin were detected in several extracts. In conclusion, we identified plant extracts with various effects in GPCR-based binding and signalling assays and identified high melatonin levels and a melatonin-potentiating activity in Pistacia vera dried fruit extracts that might be of therapeutic potential.
The Goal of Sexual Activism: Toleration, Recognition, or Both?
Authors: Nielsen Morten Ebbe Juul
Published: June 2023

Sexual activism (for, e.g., participants in the LGBT+ or BDSM communities) is prima facie commendable, at least for the liberal. However, it is unclear whether the end goal of such activism is toleration or recognition. The argument of this paper is that, on the level of authoritative political and social-moral rules, toleration is the only justifiable goal, while recognition may be pursued as an ideal outside the sphere of political and social-moral rules, that is, in civil society. The argument builds on a Gausian public reason understanding of justifiability, emphasizing reasonable disagreement and a diversity of viewpoints.
TSDSR: Temporal–Spatial Domain Denoise Super-Resolution Photon-Efficient 3D Reconstruction by Deep Learning
Authors: Tong Ziyi , Jiang Xinding , Hu Jiemin , Xu Lu , Wu Long , Yang Xu , Zou Bo
Published: June 2023

The combination of a single-photon avalanche diode detector with a high-sensitivity and photon-efficient reconstruction algorithm can realize the reconstruction of target range image from weak light signal conditions. The limited spatial resolution of the detector and the substantial background noise remain significant challenges in the actual detection process, hindering the accuracy of 3D reconstruction techniques. To address this challenge, this paper proposes a denoising super-resolution reconstruction network based on generative adversarial network (GAN) design. Soft thresholding is incorporated into the deep architecture as a nonlinear transformation layer to effectively filter out noise. Moreover, the Unet-based discriminator is introduced to complete the high-precision detail reconstruction. The experimental results show that the proposed network can achieve high-quality super-resolution range imaging. This approach has the potential to enhance the accuracy and quality of long-range imaging in weak light signal conditions, with broad applications in fields such as robotics, autonomous vehicles, and biomedical imaging.
Developing Sustainable Agriculture Systems in Medicinal and Aromatic Plant Production by Using Chitosan and Chitin-Based Biostimulants
Authors: Sun Wenli , Shahrajabian Mohamad Hesam , Petropoulos Spyridon A. , Shahrajabian Nazanin
Published: June 2023

Chitosan is illustrated in research as a stimulant of plant tolerance and resistance that promotes natural defense mechanisms against biotic and abiotic stressors, and its use may lessen the amount of agrochemicals utilized in agriculture. Recent literature reports indicate the high efficacy of soil or foliar usage of chitin and chitosan in the promotion of plant growth and the induction of secondary metabolites biosynthesis in various species, such as Artemisia annua, Curcuma longa, Dracocephalum kotschyi, Catharanthus roseus, Fragaria × ananassa, Ginkgo biloba, Iberis amara, Isatis tinctoria, Melissa officinalis, Mentha piperita, Ocimum basilicum, Origanum vulgare ssp. Hirtum, Psammosilene tunicoides, Salvia officinalis, Satureja isophylla, Stevia rebaudiana, and Sylibum marianum, among others. This work focuses on the outstanding scientific contributions to the field of the production and quality of aromatic and medicinal plants, based on the different functions of chitosan and chitin in sustainable crop production. The application of chitosan can lead to increased medicinal plant production and protects plants against harmful microorganisms. The effectiveness of chitin and chitosan is also due to the low concentration required, low cost, and environmental safety. On the basis of showing such considerable characteristics, there is increasing attention on the application of chitin and chitosan biopolymers in horticulture and agriculture productions.
Chamaecyparis lawsoniana Leaf Essential Oil as a Potential Anticancer Agent: Experimental and Computational Studies
Authors: Fikry Eman , Orfali Raha , Elbaramawi Samar S. , Perveen Shagufta , El-Shafae Azza M. , El-Domiaty Maher M. , Tawfeek Nora
Published: June 2023

Cancer remains one of the leading causes of death worldwide, affected by several factors including oxidative stress; and although conventional synthetic medicines have been used to treat cancer, they often result in various side effects. Consequently, there is a growing need for newer, safer and more effective alternatives, such as natural plant products. Essential oils (EOs) are one such alternative, offering a wide range of bioactivities, including antibacterial, antiviral, antioxidant, and anticancer properties. Accordingly, the objective of the present study was to investigate the chemical composition, as well as the antioxidant and anticancer properties of the leaf essential oil of Chamaecyparis lawsoniana (CLLEO) belonging to the Cupressaceae family. Totally, 59 constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. cis-Abienol, trans-ferruginol, α-cadinol, δ-muurolene and α-pinene were the major components. The in vitro cytotoxicity study against human breast (MCF-7), colon (HCT-116), lung (A-549), hepatocellular (HepG-2) carcinoma cells using MTT assay indicated a promising cytotoxic activity against all the tested cancer cells, particularly HepG-2, with significant selectivity indices. CLLEO exhibited weak antioxidant activity according to the DPPH, ABTS and FRAP assays. In silico docking of these constituents against the epidermal growth factor receptor (EGFR), the myeloid cell leukemia-1 (Mcl-1) and caspase-8 using Molecular Operating Environment (MOE) software demonstrated good binding affinities of the components with the active site of these targets. These findings suggested using CLLEO, or its individual components, as a potentially viable therapeutic option for managing cancerous conditions.
Planning Spatial Layout of a Typical Salt Tolerant Forage of Sweet Sorghum in the Yellow River Delta via Considering Resource Constraints, Nitrogen Use Efficiency, and Economic Benefits
Authors: Gao Yinan , Shao Changxiu , Liu Zhen , Sun Zhigang , Long Buju , Feng Puyu
Published: June 2023

In order to effectively address the issue of severe soil salinization in the coastal area of the Yellow River Delta, which has led to a significant number of medium and low-yield fields in this region, and to satisfy the rising demand for feed grain in China in recent years, a highly effective solution is to replace conventional crops by cultivating a specialized type of forage grass that can withstand high salinity levels and is well adapted to the local climate. This study proposed a spatial layout scheme for planting salt-tolerant forages, with the aim of providing a foundation for enhancing saline-alkali land and increasing resource utilization efficiency. The results showed that the climate conditions in the Yellow River Delta were suitable for planting sweet sorghum. With respect to soil salt content, the suitable planting regions for sweet sorghum can be classified into four categories: Suitable, moderately suitable, less suitable, and unsuitable, with soil salt concentrations of 2.62–5.25‰, 5.25–7.88‰, respectively. Concerning economic benefits, sweet sorghum’s input-output ratio (74.4%) surpasses that of cotton in high saline-alkali zones, providing a significant advantage in comparison with traditional crops. In non-saline-alkali and light saline-alkali areas, the traditional winter wheat-summer maize planting system offers higher economic benefits and nitrogen use efficiency, so it is recommended to maintain this system as the dominant agricultural model. In moderately and severe saline-alkali zones, although one-season maize exhibits greater nitrogen efficiency, its economic benefits are lower than those of sweet sorghum. Hence, it is advisable to promote one-season maize in suitable regions and introduce salt-tolerant forage, such as sweet sorghum in other areas. This approach offers novel ideas and methods for crop spatial layout planning and addresses potential feed grain shortages in the region.
Near-Zero Temperatures Arrest Movement of the Diaheliotropic Malva sylvestris
Authors: Arvaniti Elena , Levizou Efi , Kyparissis Aris
Published: June 2023

In the present study, the diaheliotropic leaf movement pattern of Malva sylvestris in relation to the impact of low temperature is presented. Seasonal measurements of movement characteristics along with important aspects of plant function, such as chlorophyll content, water potential, PSII photochemistry, and phenological parameters were performed on plants in their natural environment. During the study period, low winter temperatures and a 10-day freezing event gave insights into the plant’s response to harsh environmental conditions and the effect of the latter on leaf movement profile. Plant growth was significantly inhibited during low-temperature periods (leaf shedding) and the photosynthetic performance was seriously depressed, as judged by in vivo chlorophyll a fluorescence. Additionally, the diaheliotropic leaf movement pattern was arrested. Temperature rise in March triggered new leaf burst and expansion, enhancement of the photosynthetic performance, and the recovery of the diaheliotropic movement. The daily and seasonal profiles of the water potential were synergistically shaped by leaf movement and climatic conditions. We conclude that diaheliotropism of M. sylvestris is a dynamic process that coordinates with the prevailing temperatures in ecosystems like the studied one, reaching a full arrest under near-zero temperatures to protect the photosynthetic apparatus from over-excitation and prevent photoinhibition.
Growth and Performance of Guar (Cyamopsis tetragonoloba (L.) Taub.) Genotypes under Various Irrigation Regimes with and without Biogenic Silica Amendment in Arid Southwest US
Authors: Garcia Alonso , Grover Kulbhushan , VanLeeuwen Dawn , Stringam Blair , Schutte Brian
Published: June 2023

Guar is a potential crop that can be grown as a forage or as a seed crop in arid to semi-arid regions due to its low water requirements and tolerance to heat. Optimizing irrigation water use is important for making alternative crops such as guar a sustainable option. Amendments such as biogenic silica, a sedimentary rock from a biogenic source such as fossils, may help plants tolerate water stress due to reduced irrigation. The objective of the current study was to evaluate seed yield and attribute components and agronomic and physiological parameters for four guar genotypes (Matador, Kinman, Lewis, and NMSU 15-G1) under five drip irrigation regimes (I1-normal irrigation, I2-no irrigation at 75% pod formation, I3-no irrigation at 50% and 75% pod formation, I4-terminate irrigation at flowering, and I5-terminate irrigation at flowering + biogenic silica amendment) at Las Cruces in southern New Mexico, USA, from 2016 to 2018. On average, the I1 irrigation regime produced the highest guar seed yield (2715 kg ha−1) followed by I5 (2469 kg ha−1) from 2016 to 2018. As compared to the I1 regime, the I2 and I3 regimes resulted in a 20.8% and 23.4% decline in guar seed yield, respectively, on average from 2016 to 2018. The results suggest that the addition of biogenic silica might help to improve guar seed yield under reduced irrigation conditions and can produce comparable yields with an average of 300 mm of irrigation during the growing season in the southern New Mexico region of the Southwest US.
Review of Plasma Processing for Polymers and Bio-Materials Using a Commercial Frequency (50/60 Hz)-Generated Discharge
Authors: Kim Hong Tak , Jung Cheol Min , Kim Se Hyun , Lee Sung-Youp
Published: June 2023

This manuscript introduces the properties and diverse applications of plasma generated using commercial frequencies of 50/60 Hz. Commercial frequency (CF) derived plasma exhibits characteristics similar to DC discharge but with an electrical polarity and a non-continuous discharge. Due to the low-frequency nature, the reactor configurations usually are capacitively coupled plasma type. The advantages of this method include its simple power structure, low-reaction temperature, and low substrate damage. The electrical polarity can prevent charge buildup on the substrates and deposited films, thereby reducing substrate damage. The simple, low-cost, and easy-to-operate power structure makes it suitable for laboratory-scale usage. Additionally, the various applications, including plasma-enhanced vapor deposition, sputtering, dielectric barrier discharge, and surface modification, and their outcomes in the CF-derived plasma processes are summarized. The conclusion drawn is that the CF-derived plasma process is useful for laboratory-scale utilization due to its simplicity, and the results of the plasma process are also outstanding.
Correction: Yang et al. Construction and Evaluation of Chitosan-Based Nanoparticles for Oral Administration of Exenatide in Type 2 Diabetic Rats. Polymers 2022, 14, 2181
Authors: Yang Jian-Miao , Wu Lin-Jie , Lin Meng-Ting , Lu Yi-Ying , Wang Tian-Tian , Han Min , Zhang Bin , Xu Dong-Hang
Published: June 2023

The authors wish to make the following corrections to this paper [...]
Development and Characterization of Polylactic Acid (PLA)-Based Nanocomposites Used for Food Packaging
Authors: Moldovan Andrei , Cuc Stanca , Prodan Doina , Rusu Mircea , Popa Dorin , Taut Adrian Catalin , Petean Ioan , Bomboş Dorin , Doukeh Rami , Nemes Ovidiu
Published: June 2023

The present study is focused on polylactic acid (PLA) blending with bio nanoadditives, such as Tonsil® (clay) and Aerosil®, to obtain nanocomposites for a new generation of food packaging. The basic composition was enhanced using Sorbitan oleate (E494) and Proviplast as plasticizers, increasing the composite samples’ stability and their mechanical strength. Four mixtures were prepared: S1 with Tonsil®; S2 with Aerosil®; S3 with Aerosil® + Proviplast; and S4 with Sabosorb. They were complexly characterized by FT-IR spectroscopy, differential scanning calorimetry, mechanical tests on different temperatures, and absorption of the saline solution. FTIR shows a proper embedding of the filler component into the polymer matrix and DSC presents a good stability at the living body temperature for all prepared samples. Micro and nanostructural aspects were evidenced by SEM and AFM microscopy, revealing that S3 has the most compact and uniform filler distribution and S4 has the most irregular one. Thus, S3 evidenced the best diametral tensile strength and S4 evidenced the weakest values. All samples present the best bending strength at 18 °C and fair values at 4 °C, with the best values being obtained for the S1 sample and the worst for S4. The lack of mechanical strength of the S4 sample is compensated by its best resistance at liquid penetration, while S1 is more affected by the liquid infiltrations. Finally, results show that PLA composites are suitable for biodegradable and disposable food packages, and the desired properties could be achieved by proper adjustment of the filler proportions.
Optimization of Approaches to Analysis of Lignin by Thermal Decomposition
Authors: Pokryshkin Sergey , Sypalova Yuliya , Ivahnov Artem , Kozhevnikov Aleksandr
Published: June 2023

The ratio of monomeric units is one of the main characteristics of lignin, which affects the possibilities and strategies for further processing. Pyrolytic and thermal desorption decomposition of lignins followed by mass detection of macromolecule fragments are the most common methods for determining the amount of lignin structural units. Two methods of thermal decomposition of lignin were studied: thermal desorption–GC/MS (TD–GC/MS) and pyrolysis–GC/MS (Py–GC/MS). It was noted that, when using different thermal decomposition modes, the composition of the products changes, which affects the accuracy of determining the amount of lignin structural fragments. This article investigated the influence of the sample weight, the thermal decomposition temperature, and the duration of the process in various modes on the quantitation of the lignin structural units. The optimal process conditions were established. It was shown that the DS–Py–GC/MS with cryofocusing, a sample weight of 0.2–0.4 mg, and heating from 50 to 400 °C at a rate of 120 °C/min are preferable. The HSQC NMR was used as a comparison method to obtain the content of the S/G/H units. The results showed the applicability of the proposed approaches to hardwood lignins close to native.
Chitosan: Properties and Its Application in Agriculture in Context of Molecular Weight
Authors: Román-Doval Ramón , Torres-Arellanes Sandra P. , Tenorio-Barajas Aldo Y. , Gómez-Sánchez Alejandro , Valencia-Lazcano Anai A.
Published: June 2023

Chitosan is a naturally occurring compound that can be obtained from deacetylated chitin, which is obtained from various sources such as fungi, crustaceans, and insects. Commercially, chitosan is produced from crustaceans. Based on the range of its molecular weight, chitosan can be classified into three different types, namely, high molecular weight chitosan (HMWC, >700 kDa), medium molecular weight chitosan (MMWC, 150–700 kDa), and low molecular weight chitosan (LMWC, less than 150 kDa). Chitosan shows several properties that can be applied in horticultural crops, such as plant root growth enhancer, antimicrobial, antifungal, and antiviral activities. Nevertheless, these properties depend on its molecular weight (MW) and acetylation degree (DD). Therefore, this article seeks to extensively review the properties of chitosan applied in the agricultural sector, classifying them in relation to chitosan’s MW, and its use as a material for sustainable agriculture.
Changes in Carcass Composition, Meat Quality Traits, and Stress Levels in Culled Sows Exposed to Different On-Farm Rest Periods
Authors: Gvozdanović Kristina , Djurkin Kušec Ivona , Đidara Mislav , Blažetić Senka , Komlenić Miodrag , Kuterovac Krešimir , Radišić Žarko , Kušec Goran
Published: June 2023

The study investigated the effect of on-farm rest period (RP) on carcass composition, meat quality, and stress indicators of culled sows. The study was conducted on 84 Large White × Landrace culled sows, which were divided into seven groups (n = 12) with respect to the duration of the on-farm rest period: no rest period (N-RP) group and 1 to 6 weeks of on-farm rest period (1–6 RP). After completion of the on-farm RP, each group of culled sows was slaughtered and the carcass and meat quality traits were determined. Sows from 6-RP had a significantly higher dressing percentage and backfat thickness and, together with sows from 5-RP, a higher shoulder weight. The highest shoulder percentage was found in the 3-RP and 4-RP groups, while 1-RP had the highest percentage for the belly–rib part. At the same time, RP influenced the final pH values (pH24 SM and pH24 LL) measured in the longissimus thoracis et lumborum (LT) and in the musculus semimembranosus (SM), as well as drip loss, thawing loss, cooking loss, and tenderness. As far as the stress indicators are concerned, statistical differences between groups were only found in the cortisol level, which was lowest in 6-RP. The results show that RP has a positive effect on meat quality traits and carcass composition. In addition, prolonged RP has a positive effect on the levels of stress indicators.
The Impact of Industry 4.0 on the Steel Sector: Paving the Way for a Disruptive Digital and Ecological Transformation
Authors: Tolettini Laura , Di Maria Eleonora
Published: June 2023

Since the creation of a common term to indicate a set of incremental and disruptive digital technologies, Industry 4.0 has challenged European manufacturers to find a way to concretely exploit these innovations in their own business strategy. During this journey, Industry 4.0 has recently highlighted some evidence about its efficacy in enabling strategic goals on the three dimensions (economical, environmental, social) of sustainable development, which is a key element for the European Union’s goal to make manufacturers become carbon neutral until 2030. Industry 4.0 and sustainability are together affecting manufacturers’ business models, forcing managers to take chances and face challenges within their organization and in their supply-chain. As an energy-intensive sector, steel industries will be intensively affected by sustainability paradigms. With 19 qualitative interviews in the organization and supply chain of an internationalized steel producer, Feralpi Group, we provide evidence that, beyond the use of main strategic technologies (Internet of Things and Big Data analysis), the implementation of a sustainability strategy is also possible through the creation of new partnerships beyond the own supply chain. The combination of Industry 4.0 technologies and sustainability strategies, especially concerning the environment through Circular Economy practices, pushes steel industries to revise their business models, paving the way for unexpected collaborations, where suppliers, customers, and even more diverse stakeholders such as competitors could bring benefits to the company sustainable economic growth and durability.
On the Relationship between Design and Evolution
Authors: Dilley Stephen , Luskin Casey , Miller Brian , Reeves Emily
Published: June 2023

A longstanding question in science and religion is whether standard evolutionary models are compatible with the claim that the world was designed. In The Compatibility of Evolution and Design, theologian E. V. Rope Kojonen constructs a powerful argument that not only are evolution and design compatible, but that evolutionary processes (and biological data) strongly point to design. Yet Kojonen’s model faces several difficulties, each of which raise hurdles for his understanding of how evolution and design can be harmonized. First, his argument for design (and its compatibility with evolution) relies upon a particular view of nature in which fitness landscapes are “fine-tuned” to allow proteins to evolve from one form to another by mutation and selection. But biological data run contrary to this claim, which poses a problem for Kojonen’s design argument (and, as such, his attempt to harmonize design with evolution). Second, Kojonen appeals to the bacterial flagellum to strengthen his case for design, yet the type of design in the flagellum is incompatible with mainstream evolutionary theory, which (again) damages his reconciliation of design with evolution. Third, Kojonen regards convergent evolution as notable positive evidence in favor of his model (including his version of design), yet convergent evolution actually harms the justification of common ancestry, which Kojonen also accepts. This, too, mars his reconciliation of design and evolution. Finally, Kojonen’s model damages the epistemology that undergirds his own design argument as well as the design intuitions of everyday “theists on the street”, whom he seeks to defend. Thus, despite the remarkable depth, nuance, and erudition of Kojonen’s account, it does not offer a convincing reconciliation of ‘design’ and ‘evolution’.