bluuebunny/crossref_metadata_2025 huggingface dataset:
Luca Vitone: Monitoring of Four Living Canvases
Authors: Zaratti Camilla , Brunetti Simona , Fondi Veronica , Alisi Chiara , Prestileo Fernanda , de Caro Tilde , Montorsi Stefania , Macchia Andrea
Published: March 2024

“Atmospheric” painting stands as a new frontier in contemporary art, presenting challenges and new considerations in museum practices. This article aims to analyze the possible evolution of four atmospheric paintings created by artist Luca Vitone during their transition from exhibition halls to the storage facilities of the MAXXI museum. The presence of active microbiological consortium raises question regarding the potential interaction these artworks may have with the surrounding environment. The year-long monitoring involved macroscopic and microscopic observation, spectrophotometric analyses, and cell vitality assessments using a bioluminometer. Additionally, the collected data were correlated with the prevailing microclimate within the storage. Indeed, the canvases underwent an evolution during their transfer to the storage; it was mainly the photosynthetic part of the consortium that underwent the greatest changes, resulting in changes in the color tones of the canvases. The viability of the cells in the consortium also underwent changes as indicated by measurements obtained with the bioluminometer. The monitoring has provided invaluable insights into the dynamic evolution of Vitone’s artworks and allowed the authors to hypothesize some strategies for the conservation of these types of artworks.
Influence of Japanese Namban Lacquer in New Spain, Focusing on Enconchado Furniture
Authors: Kawamura Yayoi , García Barrios Ana
Published: March 2024

This work studies one type of artistic production from the Viceroyalty of New Spain during the second half of the seventeenth century and the beginning of the following century. Furniture decorated with the technique of enconchado is studied to point out the influence of Namban lacquer as well as the relationship with enconchado painting, a better known and studied corpus than furniture, and also its link to Mexican lacquer, maque. The identification and study of three pieces of furniture that feature the enconchado technique, and a cross, probably decorated with maque, offer a complementary vision of the art of enconchado and allow us to consider enconchado furniture an independent artistic genre.
Advances in Lead-Barium-Zinc-Silicate-Type Glazed Warming Bowl Related to the Chinese Xuande Reign (1426–1435)
Authors: Amadori Maria Letizia , Cardellini Serse , Mengacci Valeria
Published: March 2024

Diagnostic investigations were carried out on a rare Chinese polychrome glazed ceramic dating back to the reign of the Xuande Emperor (1426–1435). The double-walled warming bowl was investigated using several non-invasive methods such as portable optical microscopy, endoscopy, portable X-ray fluorescence spectrometry, X-radiography, and computed tomography. One microsample was collected and analyzed by scanning electron microscopy with an energy dispersive X-ray detector. According to the results, the chemical composition of the paste suggested a porcelain typology, while the glaze belongs to the lead–barium–silicate (PbO-BaO-ZnO-SiO2) system. These unexpected data contrast with common knowledge, which attests that the addition of barium in glass and ceramics manufacturing disappeared soon after the Han dynasty (206 BCE-220 CE). Moreover, the combination of PbO-BaO-ZnO-SiO2 seems to be quite rare both in ancient pre-Han times and during the Ming and Qing dynasties. This paper aims to demonstrate that (a) the use of barium for glaze and glass composition, which seems to have its roots in Taoist alchemy, was not totally halted in later periods compared to the Han dynasty; (b) lead–barium–zinc–silicate glaze was used during the Xuande Emperor’s reign. Through a review of ancient Chinese literary sources, we found a lot of unpublished information on the use of barium, lead, and zinc in the production of glazed ceramics during this period. The polychrome glazed warming bowl suggests a particular production that flourished during the brief reign of the Xuande Emperor.
Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia
Authors: Mrkvová Michaela , Kemenczeiová Jana , Achs Adam , Alaxin Peter , Predajňa Lukáš , Šoltys Katarína , Šubr Zdeno , Glasa Miroslav
Published: March 2024

Analysis of the viromes of three symptomatic Fabaceae plants, i.e., red clover (Trifolium pratense L.), pea (Pisum sativum L.), and common bean (Phaseolus vulgaris L.), using high-throughput sequencing revealed complex infections and enabled the acquisition of complete genomes of a potyvirus, bean yellow mosaic virus (BYMV). Based on phylogenetic analysis, the Slovak BYMV isolates belong to two distinct molecular groups, i.e., VI (isolate FA40) and XI (isolates DAT, PS2). Five commercial pea genotypes (Alderman, Ambrosia, Gloriosa, Herkules, Senator) were successfully infected with the BYMV-PS2 inoculum and displayed similar systemic chlorotic mottling symptoms. Relative comparison of optical density values using semi-quantitative DAS-ELISA revealed significant differences among virus titers in one of the infected pea genotypes (Ambrosia) when upper fully developed leaves were tested. Immunoblot analysis of systemically infected Alderman plants showed rather uneven virus accumulation in different plant parts. The lowest virus accumulation was repeatedly detected in the roots, while the highest was in the upper part of the plant stem.
Comparative Metabolomic and Transcriptomic Analysis Reveals That Variations in Flavonoids Determine the Colors of Different Rambutan Cultivars
Authors: Wang Jiaqi , Zhu Wencan , Yang Chengkun , Li Maofu , Feng Shun , Tang Lizhu , Yang Chengchao , Cui Zhifu
Published: March 2024

Rambutan is a tropical tree and its fruit has several favorable characteristics. To understand how the color of the rambutan fruit peel develops, the transcriptome, flavonoid metabolome, and carotenoid metabolome data of two rambutan cultivars, ‘BY2’ and ‘BY7’, which show yellow and red peels at maturity, respectively, were comprehensively analyzed at three developmental stages. We identified 26 carotenoid components and 53 flavonoid components in these cultivars. Anthocyanins were the main component contributing to the red color of ‘BY7’ after reaching ripeness. The carotenoid content decreased sharply as the fruit matured. Hence, we speculated that flavonols were the main contributors to the yellow color of the ‘BY2’ peel. In total, 6805 differentially expressed genes were screened by transcriptome analysis; the majority of them were enriched in metabolic pathways and the biosynthesis of secondary metabolites. Weighted gene co-expression network analysis results revealed that in addition to MYB and bHLH, ERF, WRKY, MYB-related, and C3H were the main potential transcription factors regulating the color of the rambutan peel. In addition, we also identified 12 structural genes associated with flavonoid biosynthesis. The research findings shed light on the molecular mechanisms of color acquisition in rambutan fruit peels, laying the foundation for the quality control of rambutan and the cultivation of differently colored cultivars of rambutan.
Effect of Temperature on Photosynthetic Pigment Degradation during Freeze–Thaw Process of Postharvest of Celery Leaves
Authors: Chen Chen , Wang Li-Xiang , Li Meng-Yao , Tan Guo-Fei , Liu Yan-Hua , Liu Pei-Zhuo , Li Ya-Peng , Liu Hui , Zhuang Jing , Tao Jian-Ping , Xiong Ai-Sheng
Published: March 2024

Celery (Apium graveolens L.) is a kind of green leaf vegetable with a large consumption demand in the food industry. It is a commonly used material in quick-frozen food stuffing such as dumplings and steamed stuffed. Fresh celery leaf blades and petioles are rich in photosynthetic pigments including chlorophyll and carotenoid, their contents are closely related to the quality of celery and its products. In order to explore the effects of freezing and thawing temperature and thawing time on the degradation of photosynthetic pigments in celery leaf blades and petioles, the changes in photosynthetic pigments during thawing storage were measured under different freezing and thawing temperatures. The results showed that lower freezing and thawing temperatures were beneficial to the preservation of photosynthetic pigments in celery leaf blades and petioles, and the loss of photosynthetic pigments enhanced with the increase in thawing temperature and thawing time. Under the cold storage condition of −80 °C, the loss rate of pigment substances can be reduced by nearly 20% compared with that of −18 °C, and −80 °C and 4 °C could be the best temperature combination of freezing and thawing. The content and degradation rate of photosynthetic pigments in celery leaf blades were higher than that in petioles during thawing, with a total chlorophyll loss rate reaching 35% during 6 to 12 h after thawing. The increase in temperature difference between freezing and thawing could aggravate the damage to the cell structure and the degradation of the pigment, as chlorophyll is more sensitive to temperature changes, and the degradation rate is significantly higher than that of carotenoids. From the perspective of delaying the degradation of photosynthetic pigments, the results of this study will provide potential references for the reasonable configuration of freezing and thawing temperatures in the process of storage and transportation of celery products.
Complete Genome and Comprehensive Analysis of Knorringia sibirica Chloroplast
Authors: Qi Kai , Chen Zhenjiang , Li Xiuzhang , Malik Kamran , Li Chunjie
Published: March 2024

Knorringia sibirica is a typical species of Polygonaceae with high medicinal and ecological value. However, there are few available phylogenetic and genetic studies about the chloroplast genome of K. sibirica. In the current study, a comprehensive examination of the chloroplast genome of K. sibirica was performed. The K. sibirica chloroplast genome is 161,384 bp, circular with a typical quadripartite structure, and comprised of guanine + cytosine content of 37.63%. The genome consists of 131 genes, including 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Seventy simple sequence repeats were identified in the genome. When compared with three closely related species, the result revealed that the chloroplast genome of K. sibirica was conserved in genome composition and structure. The specific sites in the genome suitable for DNA barcodes were identified by calculation of the nucleotide diversity. Finally, a phylogenetic tree of 49 species in Polygonaceae was constructed using the whole chloroplast genome. The phylogenetic analysis suggested that Knorringia is an independent genus in the Polygonea tribe. This research could provide support for the utilization of genetic sources and the evolutionary study of K. sibirica.
Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress
Authors: Wu Jiuyun , Zhong Haixia , Ma Yaning , Bai Shijian , Yadav Vivek , Zhang Chuan , Zhang Fuchun , Shi Wei , Abudureheman Riziwangguli , Wang Xiping
Published: March 2024

High temperatures significantly affect the growth and development of grapevines, cause irreversible damage to plants, and severely impact grape production and quality. Biostimulants can promote the growth of plants and enhance their resistance to adverse stress. However, the effects of biostimulants on grapevines under high temperatures have not been studied in detail. To analyze the effects of various biostimulants on the growth and development of grape seedlings under high temperatures, we measured chlorophyll fluorescence parameters with observed seedling phenotypes under high temperatures in open field conditions in Turpan. We conducted a comprehensive analysis of the effects of different biostimulants on the growth, development, and photosynthesis of grapevine seedlings. Our study aimed to provide scientific evidence to improve cultivation methods for grapevines under high-temperature stress. The results revealed that biostimulants have a positive effect on promoting the growth of grapevine seedlings under high-temperature stress conditions. They also positively affect the accumulation of chlorophyll components in grapevine leaves, inhibiting chlorophyll degradation and maintaining photosynthesis. However, the effects of different biostimulants were inconsistent. A comprehensive analysis revealed the following effectiveness order: T2 > T1 > T3 > Control. These findings suggest that T2 is the most effective in alleviating high-temperature stress and promoting grapevine growth. We recommend the use of T2 to improve the cultivation of grapevine seedlings during high-temperature periods. This has implications for grape production in hot and arid climatic areas.
Synthetic Seed Production and Slow Growth Storage of In Vitro Cultured Plants of Iris pallida Lam.
Authors: Meucci Annalisa , Ghelardi Cristina , Chietera Giorgiana , Mensuali Anna
Published: March 2024

Iris pallida Lam. is traditionally cultivated in Italy to sell its rhizomes to perfume-producing industries and is particularly sought-after because of its high content of irones, ketone compounds responsible for the violet smell of the orris essence. One of the critical aspects of its cultivation is the propagation method, performed by subdividing and replanting sections of the rhizome, which leads to the sacrifice of salable material. A solution is provided via in vitro propagation using the somatic embryogenesis technique, an effective method that allows the production of plants without the use of the rhizome. To facilitate the scale up of the activities of micropropagation companies, the method of slow growth storage (SGS) for orris plantlets and a somatic embryo encapsulation technique were developed for the first time. Orris plantlets were placed at 4 °C in the dark for 30, 60, 90 and 120 days and monitored 7 and 30 days after treatment. Synthetic seeds were obtained by encapsulating somatic orris embryos in sodium alginate beads, which were stored for 14 and 28 days at 4 °C and 24 °C. The results showed that it is possible to cold-preserve orris plantlets for up to 90 days without significant damages and that orris synthetic seeds can be produced and stored for a short-to-mid-term period. These conservation techniques can be useful for germplasm conservation and can also be integrated in the micropropagation cycle of orris, helping to solve issues related to the traditional propagation method.
Multilayer Silk Sericin-Based Coating for Controlled Release of Water and Nutrients in Soil: Development, Characterization, and Performance Evaluation in Agricultural Production Model
Authors: Jaramillo-Quiceno Natalia , Carmona Dora M. , Torres-Taborda Mabel , Hincapié-Llanos Gustavo Adolfo , Álvarez-López Catalina
Published: March 2024

The use of fertilizers coated with insoluble organic compounds is a promising approach for enhancing fertilizer efficiency and crop yield. Silk sericin (SS) is a protein with a high potential for the development of materials oriented toward fertilizer coating and soil amendment because of its biodegradability and the fact that it represents an important source of nitrogen for plants. Thus, this study proposes the design and evaluation of a novel SS-based multilayer coating for fertilizer granules. A pan-coating process was applied to form two distinct layers on the granules: an inner layer made of silk sericin/polyvinyl alcohol, SS/PVA (50/50 w/w), which has low solubility and porosity, and an outer hydrogel layer of SS/PVA with carboxymethyl cellulose CMC (SS/CMC/PVA 45/25/30 w/w/w). Scanning electron microscopy (SEM) was employed for the morphological characterization of the coated fertilizer (CF), examining both the cross-section and surface, while SEM with energy-dispersive X-ray spectroscopy (SEM/EDS) was used to analyze the chemical composition of the surface. The ability of the coating to reduce the nutrient-release rate was studied using water- and soil-release tests. Furthermore, its performance was evaluated in in vivo assays using jalapeño bell pepper (Capsicum annum) plants. The results revealed that the structure and composition of the multilayer coating significantly influenced its ability to delay nutrient release in both water and soil. Moreover, the inclusion of SS in the coating potentially contributed to the increased nitrogen content in the soil, thereby improving plant growth rates.
Polyphenol-Rich Extract from ‘Limoncella’ Apple Variety Ameliorates Dinitrobenzene Sulfonic Acid-Induced Colitis and Linked Liver Damage
Authors: Lama Stefania , Pagano Ester , Borrelli Francesca , Maisto Maria , Tenore Gian Carlo , Nanì Maria Francesca , Chacon-Millan Pilar , Novellino Ettore , Stiuso Paola
Published: March 2024

Inflammatory bowel conditions can involve nearly all organ systems and induce pathological processes through increased oxidative stress, lipid peroxidation and disruption of the immune response. Patients with inflammatory bowel disease (IBD) are at high risk of having extra-intestinal manifestations, for example, in the hepatobiliary system. In 30% of patients with IBD, the blood values of liver enzymes, such as AST and ALT, are increased. Moreover, treatments for inflammatory bowel diseases may cause liver toxicity. Apple polyphenol extracts are widely acknowledged for their potential antioxidant effects, which help prevent damage from oxidative stress, reduce inflammation, provide protection to the liver, and enhance lipid metabolism. The aim of this study was to investigate whether the polyphenol apple extract from Malus domestica cv. ‘Limoncella’ (LAPE) may be an effective intervention for the treatment of IBD-induced hepatotoxicity. The LAPE was administrated in vivo by oral gavage (3–300 mg/kg) once a day for 3 consecutive days, starting 24 h after the induction of dinitro-benzenesulfonic acid (DNBS) colitis in mice. The results showed that LAPE significantly attenuated histological bowel injury, myeloperoxidase activity, tumor necrosis factor and interleukin (IL-1β) expressions. Furthermore, LAPE significantly improved the serum lipid peroxidation and liver injury in DNBS-induced colitis, as well as reduced the nuclear transcription factor-kappaB activation. In conclusion, these results suggest that LAPE, through its antioxidant and anti-inflammatory properties, could prevent liver damage induced by inflammatory bowel disease.
The Role of Peptides in Asthma–Obesity Phenotype
Authors: Russjan Ewelina
Published: March 2024

The co-occurrence of asthma and obesity is becoming an increasingly common health problem. It became clear that both diseases are closely related, since overweight/obesity are associated with an increased risk of asthma development, and more than half of the subjects with severe or difficult-to-treat asthma are obese. Currently, there are no specific guidelines for the treatment of this group of patients. The mechanisms involved in the asthma–obesity phenotype include low-grade chronic inflammation and changes in pulmonary physiology. However, genetic predispositions, gender differences, comorbid conditions, and gut microbiota also seem to be important. Regulatory peptides affect many processes related to the functioning of the respiratory tract and adipose tissue. Adipokines such as leptin, adiponectin, resistin, and the less studied omentin, chemerin, and visfatin, as well as the gastrointestinal hormones ghrelin, cholecystokinin, glucagon-like peptide-1, and neuropeptides, including substance P or neuropeptide Y, can play a significant role in asthma with obesity. The aim of this article is to provide a concise review of the contribution of particular peptides in inflammatory reactions, obesity, asthma, and a combination of both diseases, as well as emphasize their potential role in the effective treatment of the asthma–obesity phenotype in the future.
Silica Microparticles from Sugarcane By-Products as an Encapsulation System for Retinoids Aimed at Topical Sustained Release
Authors: Costa Joana R. , Costa Ana Helena , Azevedo-Silva João , Tavares-Valente Diana , Sousa Sérgio C. , Neto Tânia , Pintado Manuela E. , Madureira Ana Raquel
Published: March 2024

The encapsulation of retinol within silica microparticles has emerged as a promising opportunity in the realm of cosmetic and pharmaceutical formulations, driven by the need to reinforce the photoprotection and oxidation stability of retinol. This work examines the process of encapsulating retinol into silica microparticles. The association efficiency, microparticle size, molecular structure, morphology, oxidation, and release profile, as well as biocompatibility and skin sensitization, were evaluated. Results showed that 0.03% of retinol and 9% of emulsifier leads to an association efficiency higher than 99% and a particle size with an average of 5.2 µm. FTIR results indicate that there is an association of retinol with the silica microparticles, and some may be on the surface. Microscopy indicates that when association happens, there is less aggregation of the particles. Oxidation occurs in two different phases, the first related to the retinol on the surface and the second to the associated retinol. In addition, a burst release of up to 3 h (30% free retinol, 17% associated retinol) was observed, as well as a sustained release of 44% of retinol up to 24 h. Encapsulation allowed an increase in the minimal skin cytotoxic concentrations of retinol from 0.04 μg/mL to 1.25 mg/mL without skin sensitization. Overall, retinol is protected when associated with silica microparticles, being safe to use in cosmetics and dermatology.
A Review of Approaches to Potentiate the Activity of Temozolomide against Glioblastoma to Overcome Resistance
Authors: Karve Aniruddha S. , Desai Janki M. , Gadgil Sidharth N. , Dave Nimita , Wise-Draper Trisha M. , Gudelsky Gary A. , Phoenix Timothy N. , DasGupta Biplab , Yogendran Lalanthica , Sengupta Soma , Plas David R. , Desai Pankaj B.
Published: March 2024

A glioblastoma (GBM) is one of the most aggressive, infiltrative, and treatment-resistant malignancies of the central nervous system (CNS). The current standard of care for GBMs include maximally safe tumor resection, followed by concurrent adjuvant radiation treatment and chemotherapy with the DNA alkylating agent temozolomide (TMZ), which was approved by the FDA in 2005 based on a marginal increase (~2 months) in overall survival (OS) levels. This treatment approach, while initially successful in containing and treating GBM, almost invariably fails to prevent tumor recurrence. In addition to the limited therapeutic benefit, TMZ also causes debilitating adverse events (AEs) that significantly impact the quality of life of GBM patients. Some of the most common AEs include hematologic (e.g., thrombocytopenia, neutropenia, anemia) and non-hematologic (e.g., nausea, vomiting, constipation, dizziness) toxicities. Recurrent GBMs are often resistant to TMZ and other DNA-damaging agents. Thus, there is an urgent need to devise strategies to potentiate TMZ activity, to overcome drug resistance, and to reduce dose-dependent AEs. Here, we analyze major mechanisms of the TMZ resistance-mediated intracellular signaling activation of DNA repair pathways and the overexpression of drug transporters. We review some of the approaches investigated to counteract these mechanisms of resistance to TMZ, including the use of chemosensitizers and drug delivery strategies to enhance tumoral drug exposure.
Neutrophil Gelatinase-Associated Lipocalin for the Differentiation of Mucinous Pancreatic Cystic Lesions
Authors: Olar Miruna Patricia , Iacobescu Maria , Bolboacă Sorana D. , Pojoga Cristina , Moșteanu Ofelia , Seicean Radu , Rusu Ioana , Banc Oana , Iuga Cristina Adela , Seicean Andrada
Published: March 2024

Undetermined pancreatic cystic lesion (PCL) differentiation benefits from endoscopic ultrasound (EUS) based on morphology and cyst fluid analysis, but room for new biomarkers exists. Our aim was to assess the intracystic and serum diagnostic value of neutrophil gelatinase-associated lipocalin (Ngal) and interleukin 1 beta (IL-1β) for differentiation of PCLs. This prospective study included patients from one tertiary hospital, evaluated between April 2018 and May 2020. EUS fine-needle aspiration or pancreatic pseudocysts drainage was the source of PCL intracystic liquid. The final diagnosis was based on surgery or EUS results (morphology, cytology, glucose, and CEA—carcinoembryogenic antigen). The intracystic samples were tested for Ngal, IL-1β, glucose, and CEA, and serum for Ngal and IL-1β. We evaluated 63 cysts, 33 pseudocysts, and 30 non-inflammatory cysts. The diagnostic sensitivity and specificity for mucinous PCL was 70.8% and 92.3% for intracystic Ngal (cut-off: 500–800 ng/dL), without correlation with serum Ngal, no matter the inclusion of infected pseudocysts. After exclusion of infected pseudocysts, the sensitivity and specificity for glucose were 87% and 75%, respectively, and for CEA, they were 87.1%, and 96.8%, respectively. Intracystic Ngal shows promise in differentiating mucinous PCLs, but researchers need to conduct further studies to confirm its effectiveness. Intracystic IL-1β and serum Ngal made no diagnostic contribution.
Pediatric Solid Cancers: Dissecting the Tumor Microenvironment to Improve the Results of Clinical Immunotherapy
Authors: Belgiovine Cristina , Mebelli Kristiana , Raffaele Alessandro , De Cicco Marica , Rotella Jessica , Pedrazzoli Paolo , Zecca Marco , Riccipetitoni Giovanna , Comoli Patrizia
Published: March 2024

Despite advances in their diagnosis and treatment, pediatric cancers remain among the leading causes of death in childhood. The development of immunotherapies and other forms of targeted therapies has significantly changed the prognosis of some previously incurable cancers in the adult population. However, so far, the results in pediatric cohorts are disappointing, which is mainly due to differences in tumor biology, including extreme heterogeneity and a generally low tumor mutational burden. A central role in the limited efficacy of immunotherapeutic approaches is played by the peculiar characteristics of the tumor microenvironment (TME) in pediatric cancer, with the scarcity of tumor infiltration by T cells and the abundance of stromal cells endowed with lymphocyte suppressor and tumor-growth-promoting activity. Thus, progress in the treatment of pediatric solid tumors will likely be influenced by the ability to modify the TME while delivering novel, more effective therapeutic agents. In this review, we will describe the TME composition in pediatric solid tumors and illustrate recent advances in treatment for the modulation of immune cells belonging to the TME.
Cellular Dynamics of Fas-Associated Death Domain in the Regulation of Cancer and Inflammation
Authors: Ranjan Kishu , Pathak Chandramani
Published: March 2024

Fas-associated death domain (FADD) is an adaptor protein that predominantly transduces the apoptosis signal from the death receptor (DR) to activate caspases, leading to the initiation of apoptotic signaling and the coordinated removal of damaged, infected, or unwanted cells. In addition to its apoptotic functions, FADD is involved in signaling pathways related to autophagy, cell proliferation, necroptosis, and cellular senescence, indicating its versatile role in cell survival and proliferation. The subcellular localization and intracellular expression of FADD play a crucial role in determining its functional outcomes, thereby highlighting the importance of spatiotemporal mechanisms and regulation. Furthermore, FADD has emerged as a key regulator of inflammatory signaling, contributing to immune responses and cellular homeostasis. This review provides a comprehensive summary and analysis of the cellular dynamics of FADD in regulating programmed cell death and inflammation through distinct molecular mechanisms associated with various signaling pathways.
Targeting BTK in B Cell Malignancies: From Mode of Action to Resistance Mechanisms
Authors: Mouhssine Samir , Maher Nawar , Matti Bassam Francis , Alwan Alaa Fadhil , Gaidano Gianluca
Published: March 2024

The B cell receptor (BCR) signaling pathway plays a crucial role in B cell development and contributes to the pathogenesis of B cell neoplasms. In B cell malignancies, the BCR is constitutively active through both ligand-dependent and ligand-independent mechanisms, resulting in continuous Bruton tyrosine kinase (BTK) signaling activation, which provides a survival and proliferation advantage to the neoplastic clone. Among B cell malignancies, those in which the most significant results were obtained by treatment with BTK inhibitors (BTKi) include chronic lymphocytic leukemia, mantle cell lymphoma, lymphoplasmacytic lymphoma, and diffuse large B cell lymphoma. Covalent BTKi (namely ibrutinib, acalabrutinib, and zanubrutinib) functions by irreversibly blocking BTK through covalent binding to the cysteine residue 481 (Cys-481) in the ATP-binding domain. Despite the high efficacy and safety of BTKi treatment, a significant fraction of patients affected by B cell malignancies who are treated with these drugs experience disease relapse. Several mechanisms of resistance to covalent BTKi, including Cys-481 mutations of BTK, have been investigated in B cell malignancies. Non-covalent BTKi, such as pirtobrutinib, have been developed and proven effective in patients carrying both Cys-481-mutated and unmutated BTK. Moreover, targeting BTK with proteolysis-targeting chimeras (PROTACs) represents a promising strategy to overcome resistance to BTKi in B cell neoplasms.
Evaluating Growth and Physiological Responses of a Medicinal Plant Phyla nodiflora to Salinity
Authors: Pham Anh Cong , Vo Tuan Chau , Bui Thang Duc , Van Thi-Thao Hien , Tran Dan Quang
Published: March 2024

Phyla nodiflora is a valuable medicinal plant growing in coastal areas, hypothesizing its adaptability to salinity; however, it has not been investigated. This study, for the first time, elucidated responses in the growth of the shoots and its physiology to different soil salinity of 50–400 mM NaCl. The data showed that the shoot’s dry biomass was not affected by the salinity levels up to 100 mM, and it only decreased 33.50–56.33% compared to the control under 200–400 mM NaCl, indicating that P. nodiflora is a salt-tolerant plant that could survive under high salinity. In addition, the plant also had physiological responses which indicated its salt-induced injuries and adaptation to the salt stress. The chlorophyll a content was increased while the chlorophyll b remained unchanged under the salt stress. The proline and salt accumulation increased under the salinity, but the K+ and NO3− accumulation decreased. Moreover, increases in malondialdehyde and electrolyte leakage were observed, indicating salt-induced membrane damages. These responses suggested that the plant might evolve adaptive mechanisms to salinity. Our findings are useful information for further research in order to elucidate the salt-tolerant mechanisms and develop this plant for saline agriculture.
A Review of High-Intensity Focused Ultrasound
Authors: Turner Ben , Cranston David
Published: March 2024

For 80 years, high-intensity focused ultrasound (HIFU) has been the subject of interest in medical research. It is a non-invasive procedure that causes the death of cells in a very select area through one of two mechanisms, either heat or cavitation. While diagnostic ultrasound is well known in the medical profession and ultrasound is also used in physiotherapy, high-intensity focused ultrasound is less known but is becoming increasingly important as a non-invasive tool that can be used in many ways, including in the treatment of several cancers as well as benign uterine fibroids. Other interesting developments are underway, including its use in the treatment through an intact skull of essential tremors and the tremor associated with Parkinson’s disease, and in a modified form, it is used to target drug delivery to the brain due to its potential opening of the blood–brain barrier. The depth of penetration of HIFU is variable depending on the type of transducer used and the distance from it. Clinical trials of abdominal malignancies and benign uterine fibroids are reviewed in this article along with potential side effects of the procedure. Over the past two decades, the technology has improved considerably, and the clinical indications have broadened. The current limitations of the technology are also discussed, along with the potential advances in the field that may be made over the next decade.
Efficacy and Safety of Q-Switched 1064/532 nm Nd:YAG Lasers on Benign Hypermelanosis in Dark-Skinned Individuals—A Preliminary Study
Authors: Piccolo Domenico , Fusco Irene , Crisman Giuliana , Zingoni Tiziano , Conforti Claudio
Published: March 2024

Background: Facial hypermelanosis is a major cosmetic issue that causes severe social embarrassment and psychological pain, particularly among Asians and dark-skinned individuals. Aim: This study assesses the safety and effectiveness of Q-switched 1064/532 nm nanosecond/picosecond lasers in removing benign hypermelanosis in dark-skinned individuals, evaluating the possible associated side effects. Material and methods: A total of 30 participants (80% females and 20% males) with Fitzpatrick skin types IV–V–VI who presented superficial benign hypermelanoses on the facial and décolleté area were enrolled. All patients underwent to one to two laser treatment sessions with a 1064/532 nm Q-switched laser system. Three months after the final laser session, results were assessed by comparing before- and after-treatment photos and using a quartile scale for lesion clearance (4-point Investigator Global Assessment scale). Results: All patients observed global improvements in their pigmented lesions: 53% of patients achieved excellent clearance, 30% of patients achieved good to moderate clearance, 10% of patients achieved slight clearance, and 7% of patients did not respond to the therapy. No serious adverse event occurred. Photos showed the clinical improvement achieved at 3 months follow-up. Conclusions: The Q-switched 1064/532 nm laser proved to be a key tool for treating benign hypermelanosis in all skin types, including dark-skinned persons.
Evaluating GlicoPro Tear Substitute Derived from Helix aspersa Snail Mucus in Alleviating Severe Dry Eye Disease: A First-in-Human Study on Corneal Esthesiometry Recovery and Ocular Pain Relief
Authors: Ballesteros-Sánchez Antonio , Sánchez-González José-María , Tedesco Giovanni Roberto , Rocha-de-Lossada Carlos , Murano Gianluca , Spinelli Antonio , Mazzotta Cosimo , Borroni Davide
Published: March 2024

Background: To evaluate the effects of 10% GlicoPro tear substitute therapy in patients with severe dry eye disease (DED). Methods: In this prospective longitudinal study, 30 individuals receiving 10% GlicoPro four times daily for DED were evaluated. The ocular surface disease index (OSDI) questionnaire, average non-invasive break-up time (A-NIBUT), non-anesthetic and anesthetic corneal esthesiometry (CE), ocular pain, and the presence of conjunctivochalasis (CCH) were used as clinical endpoints. Treatment compliance using dosing diaries and AEs was assessed. Results: A significant improvement was observed in the clinical endpoints: the ΔOSDI questionnaire was −39.27 ± 13.22 [−65 to −15] points, ΔA-NIBUT was 3.10 ± 1.31 [1 to 5] s, Δnon-anesthetic CE was 14 ± 6.35 [5 to 25] mm, and Δanesthetic CE was 13 ± 5.35 [5 to 20] mm (p < 0.001 for all comparisons). Ocular pain was reduced in 92.5% of the patients at the end of the follow-up. However, there was no change in the presence of CCH. In addition, all the patients were fully compliant with the dosing and no AEs related to the use of the 10% GlicoPro tear substitute were reported. Conclusions: The 10% GlicoPro tear substitute has the potential to achieve beneficial effects in ocular surface treatments.
Translation, Adaptation and Validation of the Greek Version of the Kerlan-Jobe Orthopaedic Clinic Shoulder and Elbow Score in Greek Overhead Athletes
Authors: Paraskevopoulos Eleftherios , Christakou Anna , Karanasios Stefanos , Louka Amalia Panagiota , Pamboris George M. , Papandreou Maria
Published: March 2024

Athletes engaging in overhead activities often face injury risks, emphasising the need for reliable assessment tools. This study focused on the translation and psychometric evaluation of the Kerlan-Jobe Orthopaedic Clinic (KJOC) Shoulder and Elbow Score into Greek (Gr-KJOC) for assessing upper limb function in Greek-speaking overhead athletes. The Gr-KJOC underwent meticulous translation and adaptation processes, ensuring linguistic equivalence and cultural relevance. A sample of 60 athletes participated in the psychometric evaluation, including assessments of internal consistency, test–retest reliability, construct validity, and structural validity. The Gr-KJOC demonstrated excellent internal consistency (Cronbach’s alpha = 0.95), indicating consistent measurement of the underlying construct. Test–retest reliability was excellent (ICC = 0.95), with low measurement errors. Construct validity was confirmed through correlations with the Disabilities of the Arm, Shoulder and Hand (DASH) Questionnaire. Structural validity revealed a unidimensional structure with high explained variance (75%). No floor or ceiling effects were observed, and the questionnaire proved feasible, with an average completion time of 6 min. The Gr-KJOC emerges as a reliable, valid, and feasible tool for evaluating upper limb function in Greek-speaking overhead athletes. Its psychometric properties support its utility in clinical and research contexts, contributing to the effective assessment and management of shoulder and elbow conditions in the realm of overhead sports in Greece.
Changes in Sea Level along the South China Sea Coast Based on the Homogenized Tide Gauge Data
Authors: Li Yan , Mu Lin , You Dawei , Wang Jiaying , Niu Qianru , Liao Xiaomei
Published: March 2024

To estimate the changes in the annual mean sea level (MSL) and extreme sea levels (ESLs), the largest collection of tide gauge records from 10 tidal stations along the northern coast of the South China Sea (SCS) were analyzed in this study. Here, all the tide gauge records had been homogenized by a two-step process involving the detection of inhomogeneities, that is, breakpoints caused by non-climatic changes and the application of the adjustment. The study’s conclusions, based on the homogenized tide gauge records, can be summarized as follows: The instrument change and station relocation are the main causes for the identified inhomogeneities. From 1989 to 2018, the sea level along the SCS was at an average rate of 4.0 mm per year, as measured by the homogenized tide gauges. The ESLs from the nine tidal stations rose notably with interannual fluctuations, except for the XSA station. Additionally, the ESLs exhibited substantial decadal variations. The ESLs rose along the northern coast of the SCS and were accelerated at most stations throughout the whole study period, especially after the 1980s. There were significant positive correlations between the ESL and the annual MSL at most tide gauges. The MSL’s changes, especially long-term changes, play an important role in the change in ESLs.
Assessment of Muscle Stiffness Using the MyotonPro: Effects of Fatigue on Vastus Lateralis and Medialis Muscles
Authors: Lettner Jonathan , Graventein Lars , Hakam Hassan Tarek , Ramadanov Nikolai , Becker Roland , Prill Robert
Published: March 2024

Background: The investigation of soft tissue stiffness has garnered increasing interest due to its potential applications in detecting tissue conditions, monitoring therapy effects, and preventing sports injuries. This study utilized the MyotonPro as a reliable measurement device to assess muscle stiffness and muscle frequency in the vastus lateralis and medialis muscles of both the dominant and non-dominant legs. Methods: Sixteen young, healthy subjects (seven males and nine females, age 25 ± 3.46 years) participated in this study. To induce maximal muscle fatigue, the vastus lateralis and vastus medialis muscles were subjected to a 30 kg load using a single-leg leg press. Pre- and post-fatigue measurements were conducted by two testers on the dominant and non-dominant legs, respectively, employing the MyotonPro. Results: We revealed a significant increase in muscle stiffness after maximal muscle fatigue. Specifically, on the dominant side, the vastus lateralis exhibited a stiffness increase of 6.5%, while the vastus medialis showed a 6.3% increase. On the non-dominant side, the vastus lateralis demonstrated a 7.6% increase, and the vastus medialis exhibited a 6.7% increase in muscle stiffness. Furthermore, muscle frequency increased by 8.6% (vastus lateralis) and 13.5% (vastus medialis) on the dominant side and by 15.1% (vastus lateralis) and 6.3% (vastus medialis) on the non-dominant side. The reliability of the measurements varied, with Cronbach’s alpha values ranging from inadequate 0.49 to very good 0.88. Conclusion: This study affirms the efficacy of the MyotonPro as a measurement device for assessing muscle stiffness and establishes its reliability. The observed increase in muscle stiffness after maximal muscle fatigue, accompanied by changes in muscle frequency, underscores the device’s utility. However, further research is warranted to validate the reproducibility of these findings and explore additional facets of the muscular response to fatigue.
Macroeconomic Shocks and Economic Performance in Malaysia: A Sectoral Analysis
Authors: Thorbecke Willem
Published: March 2024

Many shocks, including COVID-19, wars, inflation, contractionary U.S. monetary policy, and oil price hikes, have recently buffeted the world economy. The literature has reported mixed results concerning how these shocks impact Malaysian stock returns. Some studies found that U.S. monetary policy mattered for Malaysia, while others reported that it did not. This paper, employing two U.S. monetary policy measures over the 2001–2019 period, finds that U.S. policy matters little for Malaysian equities. Some studies found that oil price hikes increased Malaysian stock returns while others reported that they did not. This paper, employing updated data, reports that oil price increases, driven by both world demand shocks and oil supply shocks, raise Malaysian stock returns. The paper also compares the performance of Malaysian equities since the pandemic began, with returns forecasted based on macroeconomic variables. The period since the pandemic started has been labeled the megacrisis era. Interconnected crises, including the pandemic, wars, rising commodity prices, and climate events, all overlapped. The results indicate that industrial metals and banks have performed well since the pandemic began. Food producers, healthcare providers, medical equipment suppliers, tourist-related companies, and semiconductor firms have suffered. This paper considers several steps that could help these sectors to recover.
A Comparative Study on Rice Husk, as Agricultural Waste, in the Production of Silica Nanoparticles via Different Methods
Authors: Yuan Shengwang , Hou Yihao , Liu Shun , Ma Yunhai
Published: March 2024

This study explores the conversion of agricultural waste into valuable industrial precursors, specifically focusing on the production of silica nanoparticles from rice husk (RH) via calcination and sol–gel processes. The synthesized particles underwent detailed analysis to assess their chemical composition, structural features, morphological characteristics, and size distribution. This comparative analysis evaluates the effectiveness of various methods in generating silica from RH and examines the impact of different drying techniques, including freeze-drying and conventional thermal drying, on the properties of the resulting silica nanoparticles. Utilizing a combination of sol–gel and freeze-drying techniques produced spherical nanoparticles with diameters of 10 to 20 nm, characterized by size uniformity, clear contours, and minimal aggregation. X-ray diffraction (XRD) analysis identified the amorphous nature of the silica, as evidenced by diffraction peaks typical of amorphous silica in the RH-derived samples processed via different methods. Significantly, the XRD patterns of the calcination-derived silica showed no foreign peaks, indicating a purer amorphous state. The findings of this study are anticipated to contribute to the development of innovative and efficient silica nanomaterials, fostering the sustainable use of agricultural waste.
A Study of the Superplastic Deformation Behavior of Low-Cost Ti-2Fe-0.1B Alloys
Authors: Mi Yaoyao , Lu Yu , Wang Delong , Zhao Yihui , Dong Yuecheng , Chang Hui , Alexandrov I. V.
Published: March 2024

Titanium alloys have high specific strength and corrosion resistance, which have promising applications in industry. However, the machinability of titanium alloys is limited due to their crystal lattice and physical properties. Thus, in recent years, the superplastic forming of titanium alloys has been intensively developing, in particular, forming at low temperatures and/or high strain rates. In this work, a tensile test of low-cost Ti-2Fe-0.1B alloys was carried out at a temperature of 550~750 °C and a strain rate of 1 × 10−3 s−1~1 × 10−2 s−1. The results showed that the alloy exhibited good superplasticity even at a high strain rate (1 × 10−2 s−1) and a low deformation temperature of 550 °C; the elongation of the alloy in this state reached 137.5%. The high strain rate sensitivity coefficient m (0.3) and the maximum elongation (452%) were obtained at a strain rate of 1 × 10−3 s−1 and a temperature of 750 °C. Characteristics of the microstructure showed that during superplastic deformation, the recrystallization and grain boundary sliding of the alloy phases were accelerated, which could be ascribed to the effect of the element Fe. At the same time, the TiB phase located around the primary elongated α grains could also induce dynamic recrystallization and dynamic globularization during deformation.
3D Polymer Gel Dosimeters with iCBCT 3D Reading and polyGeVero-CT Software Package for Quality Assurance in Radiotherapy
Authors: Kozicki Marek , Maras Piotr , Jaszczak-Kuligowska Malwina
Published: March 2024

Dynamically evolving radiotherapy instruments require advancements in compatible 3D dosimetry systems. This paper reports on such tools for the coincidence test of the mechanical and radiation isocenter for a medical accelerator as part of the quality assurance in routine radiotherapy practice. Three-dimensional polymer gel dosimeters were used in combination with 3D reading by iterative cone beam computed tomography and 3D data processing using the polyGeVero-CT software package. Different polymer gel dosimeters were used with the following acronyms: VIP, PAGAT, MAGIC, and NIPAM. The same scheme was used for each dosimeter: (i) irradiation sensitivity test for the iterative cone beam computed tomography reading to determine the appropriate monitor unit for irradiation, and (ii) verification of the chosen irradiation conditions by a star-shot 2D irradiation of each 3D dosimeter in the direction of performing the test. This work concludes with the optimum monitor unit per beam for each selected 3D dosimeter, delivers schemes for quick and easy determination of the radiation isocenter and performing the coincidence test.