bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenomenology of jet angularities at the LHC
Authors: Reichelt Daniel , Caletti Simone , Fedkevych Oleh , Marzani Simone , Schumann Steffen , Soyez Gregory
Published: March 2022

Abstract

We compute resummed and matched predictions for jet angularities in hadronic dijet and Z+jet events with and without grooming the candidate jets using the SoftDrop technique. Our theoretical predictions also account for non-perturbative corrections from the underlying event and hadronisation through parton-to-hadron level transfer matrices extracted from dedicated Monte Carlo simulations with Sherpa. Thanks to this approach we can account for non-perturbative migration effects in both the angularities and the jet transverse momentum. We compare our predictions against recent measurements from the CMS experiment. This allows us to test the description of quark- and gluon-jet enriched phase-space regions separately. We supplement our study with Sherpa results based on the matching of NLO QCD matrix elements with the parton shower. Both theoretical predictions offer a good description of the data, within the experimental and theoretical uncertainties. The latter are however sizeable, motivating higher-accuracy calculations.
Road map through the desert with scalars
Authors: Olivas Ubaldo Cavazos , Kowalska Kamila , Kumar Dinesh
Published: March 2022

Abstract

In the context of the gauge coupling unification, we present a comprehensive analysis of the extensions of the Standard Model with vector-like fermions and scalars. We find 145 models that satisfy the unification condition, which are distinguishable by the number of new particles in the spectrum and by their transformation properties under the gauge symmetry group of the Standard Model. For all models we derive lower bounds on the exotic fermion and scalar masses, stemming from the measurement of the strong gauge coupling scale dependence, from the heavy stable charged particle searches, and from the electroweak precision tests. We also discuss the potential of testing the unification scenarios at the future 100 TeV collider and in the proton decay experiments. We show that many models can already be excluded based on the current data, while many others will be entirely probed in the coming years.
Holographic evolution with dynamical boundary gravity
Authors: Ecker Christian , van der Schee Wilke , Mateos David , Casalderrey-Solana Jorge
Published: March 2022

Abstract

Holography has provided valuable insights into the time evolution of strongly coupled gauge theories in a fixed spacetime. However, this framework is insufficient if this spacetime is dynamical. We present a scheme to evolve a four-dimensional, strongly interacting gauge theory coupled to four-dimensional dynamical gravity in the semiclassical regime. As in previous work, we use holography to evolve the quantum gauge theory stress tensor, whereas the four-dimensional metric evolves according to Einstein’s equations coupled to the expectation value of the stress tensor. The novelty of our approach is that both the boundary and the bulk spacetimes are constructed dynamically, one time step at a time. We focus on Friedmann-Lemaître-Robertson-Walker geometries and evolve far-from-equilibrium initial states that lead to asymptotically expanding, flat or collapsing Universes.
Time-Space Fractional Diffusion Problems: Existence, Decay Estimates and Blow-Up of Solutions
Authors: Shen Ruixin , Xiang Mingqi , Rădulescu Vicenţiu D.
Published: March 2022

The aim of this paper is to study the following time-space fractional diffusion problem $$\begin{aligned} {\left\{ \begin{array}{ll} \displaystyle \partial _t^\beta u+(-\Delta )^\alpha u+(-\Delta )^\alpha \partial _t^\beta u=\lambda f(x,u) +g(x,t) &{}\text{ in } \Omega \times {\mathbb {R}}^{+},\\ u(x,t)=0\ \ &{}\text{ in } ({\mathbb {R}}^N{\setminus }\Omega )\times {\mathbb {R}}^+,\\ u(x,0)=u_0(x)\ &{}\text{ in } \Omega ,\\ \end{array}\right. } \end{aligned}$$

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. Under natural assumptions, the global and local existence of solutions are obtained by applying the Galerkin method. Then, by virtue of a differential inequality technique, we give a decay estimate of solutions. Moreover, the blow-up property of solutions is also investigated.
Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau
Authors: Lemke Nora , El-Khatib Ahmed H. , Tchipilov Teodor , Jakubowski Norbert , Weller Michael G. , Vogl Jochen
Published: March 2022

Quantitative proteomics is a growing research area and one of the most important tools in the life sciences. Well-characterized and quantified protein standards are needed to achieve accurate and reliable results. However, only a limited number of sufficiently characterized protein standards are currently available. To fill this gap, a method for traceable protein quantification using sulfur isotope dilution inductively coupled plasma mass spectrometry (ICP-MS) was developed in this study. Gel filtration and membrane filtration were tested for the separation of non-protein-bound sulfur in the protein solution. Membrane filtration demonstrated a better performance due to the lower workload and the very low sulfur blanks of 11 ng, making it well suited for high-purity proteins such as NIST SRM 927, a bovine serum albumin (BSA). The method development was accomplished with NIST SRM 927e and a commercial avidin. The quantified mass fraction of NIST SRM 927e agreed very well with the certified value and showed similar uncertainties (3.6%) as established methods while requiring less sample preparation and no species-specific standards. Finally, the developed procedure was applied to the tau protein, which is a biomarker for a group of neurodegenerative diseases denoted “tauopathies” including, e.g., Alzheimer’s disease and frontotemporal dementia. For the absolute quantification of tau in the brain of transgenic mice overexpressing human tau, a well-defined calibration standard was needed. Therefore, a pure tau solution was quantified, yielding a protein mass fraction of (0.328 ± 0.036) g/kg, which was confirmed by amino acid analysis.

Graphical abstract
Healthy Spouses can be Considered as Living Liver Donors
Authors: Lee Okjoo , Kim Jong Man , Kim Sang Jin , Rhu Jinsoo , Choi Gyu‐Seong , Joh Jae‐Won
Published: March 2022

Background

Spousal donors have gradually been accepted as an alternative living liver donors to alleviate the organ shortage and prevent donations from children. No information is available regarding the effects of spousal donation on donor safety and recipient outcomes. Our purpose in this study was to determine how spousal liver grafts in living donor liver transplantation (LDLT) affect donor safety and recipient outcomes compared with those of LDLT from children.

Methods

We retrospectively analyzed 656 patients, including spouses and children, who underwent a right or extended right hepatectomy for living liver donation between January 2009 and December 2018.

Results

Spouses represented 18.8% (n = 123) of living liver donors. Female donors comprised 78.9% (n = 97) of spousal donors, and the proportion of male donors in the children group was 72.6% (n = 387). The mean donor operation time of the spousal group was shorter than that of the children group (330 min vs. 358 min; P = 0.011), and the complication rate in the spousal group was lower than that in the children group (12.2% vs. 22.9%; P = 0.006). However, there were no differences in severe complication rates, hospitalization, or liver function tests between the 2 groups at 3 months after donor surgery. The overall survival of recipients in the spousal group was not reduced compared to that of recipients in the children group.

Conclusion

The present study suggests that, with careful selection, spousal donation is feasible and safe in LDLT.
Empirically derived thermal thresholds of four coral species along the Red Sea using a portable and standardized experimental approach
Authors: Evensen Nicolas R. , Voolstra Christian R. , Fine Maoz , Perna Gabriela , Buitrago-López Carol , Cárdenas Anny , Banc-Prandi Guilhem , Rowe Katherine , Barshis Daniel J.
Published: March 2022

Global warming is causing an unprecedented loss of species and habitats worldwide. This is particularly apparent for tropical coral reefs, with an increasing number of reefs experiencing mass bleaching and mortality on an annual basis. As such, there is a growing need for a standardized experimental approach to rapidly assess the thermal limits of corals and predict the survival of coral species across reefs and regions. Using a portable experimental system, the Coral Bleaching Automated Stress System (CBASS), we conducted standardized 18 h acute thermal stress assays to quantitively determine the upper thermal limits of four coral species across the length of the Red Sea coastline, from the Gulf of Aqaba (GoA) to Djibouti (~ 2100 km). We measured dark-acclimated photosynthetic efficiency (Fv/Fm), algal symbiont density, chlorophyll a, and visual bleaching intensity following heat stress. Fv/Fm was the most precise response variable assessed, advancing the Fv/Fm effective dose 50 (ED50, i.e., the temperature at which 50% of the initial Fv/Fm is measured) as an empirically derived proxy for thermal tolerance. ED50 thermal thresholds from the central/southern Red Sea and Djibouti populations were consistently higher for Acropora hemprichii, Pocillopora verrucosa, and Stylophora pistillata (0.1–1.8 °C above GoA corals, respectively), in line with prevailing warmer maximum monthly means (MMMs), though were lower than GoA corals relative to site MMMs (1.5–3.0 °C). P. verrucosa had the lowest thresholds overall. Despite coming from the hottest site, thresholds were lowest for Porites lobata in the southern Red Sea, suggesting long-term physiological damage or ongoing recovery from a severe, prior bleaching event. Altogether, the CBASS resolved historical, taxonomic, and possibly recent environmental drivers of variation in coral thermal thresholds, highlighting the potential for a standardized, short-term thermal assay as a universal approach for assessing ecological and evolutionary variation in the upper thermal limits of corals.
Linking titanite U–Pb dates to coupled deformation and dissolution–reprecipitation
Authors: Moser Amy C. , Hacker Bradley R. , Gehrels George E. , Seward Gareth G. E. , Kylander-Clark Andrew R. C. , Garber Joshua M.
Published: March 2022

Titanite U–Pb geochronology is a promising tool to date high-temperature tectonic processes, but the extent to and mechanisms by which recrystallization resets titanite U–Pb dates are poorly understood. This study combines titanite U–Pb dates, trace elements, zoning, and microstructures to directly date deformation and fluid-driven recrystallization along the Coast shear zone (BC, Canada). Twenty titanite grains from a deformed calc-silicate gneiss yield U–Pb dates that range from ~ 75 to 50 Ma. Dates between ~ 75 and 60 Ma represent metamorphic crystallization or inherited detrital cores, whereas ~ 60 and 50 Ma dates reflect localized, grain-scale processes that variably recrystallized the titanite. All the analyzed titanite grains show evidence of fluid-mediated dissolution–reprecipitation, particularly at grain rims, but lack evidence of thermally mediated volume diffusion at a metamorphic temperature of > 700 °C. The younger U–Pb dates are predominantly found in bent portions of grains or fluid-recrystallized rims. These features likely formed during ductile slip and associated fluid flow along the Coast shear zone, although it is unclear whether the dates represent 10 Myr of continuous recrystallization or incomplete resetting of the titanite U–Pb system during a punctuated metamorphic event. Correlations between dates and trace-element concentrations vary, indicating that the effects of dissolution–reprecipitation decoupled U–Pb dates from trace-element concentrations in some grains. These results demonstrate that U–Pb dates from bent titanite lattices and titanite subgrains may directly date crystal-plastic deformation, suggesting that deformation microstructures enhance fluid-mediated recrystallization, and emphasize the complexity of fluid and deformation processes within and among individual grains.
Application of a fuzzy verification framework for the evaluation of a regional-scale landslide early warning system during the January 2020 Gloria storm in Catalonia (NE Spain)
Authors: Palau Rosa M. , Berenguer Marc , Hürlimann Marcel , Sempere-Torres Daniel
Published: March 2022

The Gloria storm rainfalls affected Catalonia from 20 to 23 January 2020 and triggered multiple landslides, some of which affected buildings and infrastructures (such as roads and railways). This paper presents the rainfall and landslide datasets collected during the event, and evaluates the performance of a regional landslide early warning system (LEWS) during the Gloria storm applying a fuzzy verification method. The majority of the inventoried landslides can be classified as slides, involving a limited volume of sediment (up to 10 m3), and were triggered in cut slopes along linear infrastructures. Rainfall accumulations were significant in the whole region, especially in the Montseny area, where over 450 mm were registered in 96 h. Generally, the LEWS computed moderate and high warnings in the areas where large rainfall amounts were recorded, and showed good correspondence with the locations where landslides were reported. The fuzzy verification method has been applied using neighbouring windows of different sizes to obtain scale-dependant information on the LEWS performance. The skill of the LEWS considerably improves when enlarging the neighbouring window size from 500 m to 1 km.
Community financing for sustainable food and farming: a proximity perspective
Authors: Behrendt Gerlinde , Peter Sarah , Sterly Simone , Häring Anna Maria
Published: March 2022

An increasing number of small and medium-sized enterprises (SMEs) in the German organic agri-food sector involves citizens through different community financing models. While such models provide alternative funding sources as well as marketing opportunities to SMEs, they allow private investors to combine their financial and ethical concerns by directly supporting the development of a more sustainable food system. Due to the low level of financial intermediation, community financing is characterized by close relations between investors and investees. Against this background, we apply the proximity concept from economic geography to explore spatial and relational aspects of community financing in the German organic agri-food sector. Based on a qualitative multiple case study approach, we find that the relevance of proximity is twofold. While different forms of proximity between SMEs and their potential investors are key success factors, proximity is also considered as one desired outcome of community financing. Furthermore, our results reveal that the extent to which SMEs rely on particular proximity dimensions distinguishes two different approaches to community financing.
Exploring the journey of Responsible Business Model Innovation in Asian companies: A review and future research agenda
Authors: Magni Domitilla , Palladino Rosa , Papa Armando , Cailleba Patrice
Published: March 2022

Responsible Business Model Innovation is increasingly becoming a relevant challenge in academic research and business practice, mainly in the Asian context. Changes in business models are widely acknowledged as a key strategy for achieving long-term innovation. However, little is known about the design journey of Responsible Business Model Innovation. By applying the knowledge-based view and stakeholder theory, this paper introduces the founding pillars of Responsible Business Model Innovation, namely: Corporate Social Responsiveness, Inclusiveness, and Reflective Knowledge Exchange. Based on the analysis of extended bodies of literature published between 2011 and 2021 on business model innovation, sustainability innovation, stakeholder theory and responsible innovation, the article explores the state of the art of business ethics and dynamic capabilities in Asian organizations. Findings show that recent research in the field of sustainability and ethical values are improving the impact on business models, thus encouraging the advent of Responsible Business Model Innovation. This article contributes to the emerging field of responsible innovation and offers novel theoretical and practical implications for academy and practitioners, including a first attempt to develop a road map to be followed to achieve of sustainable and ethical values for business and society at large.
Multi-objective optimisation of polymerase chain reaction continuous flow systems
Authors: Zagklavara Foteini , Jimack Peter K. , Kapur Nikil , Querin Osvaldo M. , Thompson Harvey M.
Published: March 2022

A surrogate-enabled multi-objective optimisation methodology for a continuous flow Polymerase Chain Reaction (CFPCR) systems is presented, which enables the effect of the applied PCR protocol and the channel width in the extension zone on four practical objectives of interest, to be explored. High fidelity, conjugate heat transfer (CHT) simulations are combined with Machine Learning to create accurate surrogate models of DNA amplification efficiency, total residence time, total substrate volume and pressure drop throughout the design space for a practical CFPCR device with sigmoid-shape microfluidic channels. A series of single objective optimisations are carried out which demonstrate that DNA concentration, pressure drop, total residence time and total substrate volume within a single unitcell can be improved by up to$$\sim$$∼5.7%,$$\sim$$∼80.5%,$$\sim$$∼17.8% and$$\sim$$∼43.2% respectively, for the practical cases considered. The methodology is then extended to a multi-objective problem, where a scientifically-rigorous procedure is needed to allow designers to strike appropriate compromises between the competing objectives. A series of multi-objective optimisation results are presented in the form of a Pareto surface, which show for example how manufacturing and operating cost reductions from device miniaturisation and reduced power consumption can be achieved with minimal impact on DNA amplification efficiency. DNA amplification has been found to be strongly related to the residence time in the extension zone, but not related to the residence times in denaturation and annealing zones.
Compost and microbial biostimulant applications improve plant growth and soil biological fertility of a grass-based phytostabilization system
Authors: Visconti Donato , Ventorino Valeria , Fagnano Massimo , Woo Sheridan Lois , Pepe Olimpia , Adamo Paola , Caporale Antonio Giandonato , Carrino Linda , Fiorentino Nunzio
Published: March 2022

In this work, a grass-based phytoremediation system integrated with an organic amendment and biostimulants was evaluated for remediating contaminated sites. Plant growth and biological fertility were monitored to assess the efficacy of a vegetative cap used as a safety measure to reduce sanitary and environmental risks of industrially contaminated soils and soil-washing sludges. Both matrices were potentially contaminated with Pb and Zn with an ecological risk index from low to moderate. According to potentially toxic elements (PTEs) bioaccessibility tests, the exposure to the released fine particulate matter may cause serious risks to human beings, in particular to children. The grass mixture was well adapted to both the substrates and a low PTEs mobility was detected, thus, reducing the leaching risk to ground water sources. Compost addition augmented significantly nitrogenase reductase (nifH) and ammonia monooxygenase (amoA) gene expression abundance in both substrates. Furthermore, a positive interaction between compost fertilization and a Trichoderma-based biostimulant inoculation was recorded in sludges resulting in a significant stimulation of nitrogen-fixing and ammonia-oxidizing bacteria. The application of compost and biostimulant increased soil fertility and plant growth. Furthermore, there was a slight reduction in PTE bioaccessibility, thus, improving the efficiency of the phytostabilization, limiting the resuspension and dispersion of the health-risk soil particulate.
Molecular characterization of Xanthomonas species isolated from Araceae and the development of a triplex TaqMan assay for detection of Xanthomonas phaseoli pv. dieffenbachiae
Authors: van der Wolf Jan , Krijger Marjon , Mendes Odette , Brankovics Balazs , Bonants Peter , Didden Leonie , Meekes Ellis
Published: March 2022

In total 58 Xanthomonas strains isolated from Araceae worldwide, together with 13 other phylogenetically-related Xanthomonas strains, were characterized using multilocus sequence analysis based on concatenated sequences of seven single copy orthologous genes, extracted from whole genome sequences. The analysis revealed a monophyletic clade of 48 strains, 44 isolated from Anthurium, identified as X. phaseoli pv. dieffenbachiae (Xpd) confirmed by nucleotide identity analysis. The other strains from aroids were identified as Xanthomonas euvesicatoria (2 strains), X citri (5 strains) and Xanthomonas sacchari (3 strains). Two TaqMan assays were designed for specific detection of Xpd, one targeting sequences of a hypothetical protein and one targeting a type I restriction endonuclease subunit S. The two assays showed similar reaction kinetics and were merged with an assay comprising an amplification and extraction control into a triplex assay. The assay was able to detect minimally 100 copies of a target sequence delivered as a gBlock, 100 fg of genomic DNA and 104 cells per mL in an Anthurium leaf extract.
CO2 emissions and income growth in Latin America: long-term patterns and determinants
Authors: Seri C. , de Juan Fernández A.
Published: March 2022

We employ an ARDL bounds testing approach to cointegration and Unrestricted Error Correction Models to estimate the relationship between income and CO2 emissions per capita in 21 Latin American Countries (LACs) over 1960–2017. Using time series, we estimate six different specifications of the model to take into account the independent effect on CO2 emissions per capita of different factors considered as drivers of different dynamics of CO2 emissions along the development path. This approach allows to address two concerns. First, the estimation of the model controlling for different variables serves to assess if the EKC hypothesis is supported by evidence in any of the LACs considered and to evaluate if this evidence is robust to different model specifications. Second, the inclusion of control variables accounting for the effect on CO2 emissions is directed at increasing our understanding of CO2 emissions drivers in different countries. The EKC hypothesis effectively describes the long-term income-emissions relationship only in a minority of LACs and, in many cases, the effect on CO2 emissions of different factors depends on the individual country experience and on the type and quantity of environmental policies adopted. Overall, these results call for increased environmental action in the region.
Believing for a Reason is (at Least) Nearly Self-Intimating
Authors: Keeling Sophie
Published: March 2022

This paper concerns a specific epistemic feature of believing for a reason (e.g., believing that it will rain on the basis of the grey clouds outside). It has commonly been assumed that our access to such facts about ourselves is akin in all relevant respects to our access to why other people hold their beliefs. Further, discussion of self-intimation—that we are necessarily in a position to know when we are in certain conditions—has centred largely around mental states. In contrast to both assumptions, this paper argues that believing for a reason is (at least) very nearly self-intimating: necessarily, if a subject believes that q for the reason that p, then, provided relevant conceptual and rational capacities, she is in a position to form a justified true belief that she believes that q for the reason that p. We should think this on the basis of the role that believing for a reason plays from the subjects’ perspective, and in particular, the way in which it intellegises one’s belief.
The right ventricular involvement in dilated cardiomyopathy: prevalence and prognostic implications of the often-neglected child
Authors: Manca Paolo , Nuzzi Vincenzo , Cannatà Antonio , Castrichini Matteo , Bromage Daniel I. , De Luca Antonio , Stolfo Davide , Schulz Uwe , Merlo Marco , Sinagra Gianfranco
Published: March 2022

Dilated cardiomyopathy (DCM) is a primary heart muscle disease characterized by left or biventricular systolic impairment. Historically, most of the clinical attention has been devoted to the evaluation of left ventricular function and morphology, while right ventricle (RV) has been for many years the forgotten chamber. Recently, progresses in cardiac imaging gave clinicians precious tools for the evaluation of RV, raising the awareness of the importance of biventricular assessment in DCM. Indeed, RV involvement is far from being uncommon in DCM, and the presence of right ventricular dysfunction (RVD) is one of the major negative prognostic determinants in DCM patients. However, some aspects such as the possible role of specific genetic mutations in determining the biventricular phenotype in DCM, or the lack of specific treatments able to primarily counteract RVD, still need research. In this review, we summarized the current knowledge on RV involvement in DCM, giving an overview on the epidemiology and pathogenetic mechanisms implicated in determining RVD. Furthermore, we discussed the imaging techniques to evaluate RV function and the role of RV failure in advanced heart failure.
Targeting intercellular adhesion molecule-1 (ICAM-1) to reduce rhinovirus-induced acute exacerbations in chronic respiratory diseases
Authors: Shukla Shakti D. , Shastri Madhur D. , Vanka Swaroop K. , Jha Niraj Kumar , Dureja Harish , Gupta Gaurav , Chellappan Dinesh Kumar , Oliver Brian G. , Dua Kamal , Walters E. Haydn
Published: March 2022

The chronic respiratory non-communicable diseases, asthma and chronic obstructive pulmonary disease (COPD) are among the leading causes of global mortality and morbidity. Individuals suffering from these diseases are particularly susceptible to respiratory infections caused by bacterial and/or viral pathogens, which frequently result in exacerbation of symptoms, lung function decline, frequent hospital emergency visits and increased socioeconomic burden. Human rhinoviruses (HRV) remain the major viral pathogen group implicated in exacerbations of both asthma and COPD. The rhinoviral entry into the host lung epithelium is facilitated primarily by the adhesion site (“receptor”) intercellular adhesion molecule-1 (ICAM-1), coincidentally expressed on the respiratory epithelium in these conditions. Multiple observations of increased airway ICAM-1 protein in asthmatics, smokers and smoking-related COPD have been recorded in the literature. However, the lack of robust therapies for COPD in particular has triggered a renewed interest in assessing receptor antagonism-based anti-viral strategies for treatment of intercurrent viral infections in those with pre-existing chronic lung diseases. Given the crucial role ICAM-1 plays in facilitating HRV adhesion and, thus, transmissibility to the host respiratory system, as well as the up-regulation of ICAM-1 by smoking, we summarize the role of HRV in smoking-induced COPD and especially highlight the role of ICAM-1 in epithelial viral adhesion and chronic lung disease progression. Further, the review also sheds light specifically on evolving precision therapeutic strategies in blocking ICAM-1 for preventing viral adhesion and exacerbations of COPD.
RestraintMaker: a graph-based approach to select distance restraints in free-energy calculations with dual topology
Authors: Ries Benjamin , Rieder Salomé , Rhiner Clemens , Hünenberger Philippe H. , Riniker Sereina
Published: March 2022

The calculation of relative binding free energies (RBFE) involves the choice of the end-state/system representation, of a sampling approach, and of a free-energy estimator. System representations are usually termed “single topology” or “dual topology”. As the terminology is often used ambiguously in the literature, a systematic categorization of the system representations is proposed here. In the dual-topology approach, the molecules are simulated as separate molecules. Such an approach is relatively easy to automate for high-throughput RBFE calculations compared to the single-topology approach. Distance restraints are commonly applied to prevent the molecules from drifting apart, thereby improving the sampling efficiency. In this study, we introduce the program RestraintMaker, which relies on a greedy algorithm to find (locally) optimal distance restraints between pairs of atoms based on geometric measures. The algorithm is further extended for multi-state methods such as enveloping distribution sampling (EDS) or multi-site$$\lambda $$λ-dynamics. The performance of RestraintMaker is demonstrated for toy models and for the calculation of relative hydration free energies. The Python program can be used in script form or through an interactive GUI within PyMol. The selected distance restraints can be written out in GROMOS or GROMACS file formats. Additionally, the program provides a human-readable JSON format that can easily be parsed and processed further. The code of RestraintMaker is freely available on GitHubhttps://github.com/rinikerlab/restraintmaker.
Novel glass materials-based (PVA/PVP/Al2O3/SiO2) hybrid composite hydrogel membranes for industrial applications: synthesis, characterization, and physical properties
Authors: Ali Ahmed I. , Salim Samar A. , Kamoun Elbadawy A.
Published: March 2022

Metal oxides e.g., Al2O3and SiO2loaded-hydrogel blended membranes composed of poly(vinyl alcohol)/poly(vinyl pyrrolidone) (PVA/PVP, PVA/PVP/Al2O3, PVA/PVP/SiO2, PVA/PVP/Al2O3/SiO2) were successfully prepared on precleared glass plates by dip coating method. Meanwhile, series of obtained crosslinked hydride composite hydrogel membranes were successfully prepared using solution-casting method. Samples have been characterized for use in microelectronic devices. Results of X-ray diffraction revealed that the structure of doped sample with nanoparticle has a polycrystalline structure (hexagonal and Orthorhombic), while FE-SEM micrographs show grains in nanoscale and homogenous in nature of membranes. Interestingly, optical measurements of composites blended membranes were recorded using UV/Vis spectrometer. The optical parameters such as refractive index and optical energy gap were estimated. Moreover, complex dielectric constants were calculated optically for all composites, the experimental data shows the additive of nanoparticles composites has a direct energy band gap. Where,Egfor PVA/PVP/SiO2, PVA/PVP/Al2O3and PVA/PVP/Al2O3/SiO2at 1.82, 2.55, and 1.95 eV), respectively. While the sample PVA/PVP has an indirect band gapEgof value 2.24 eV. Finally, the frequency dependence of the transport properties was measured, where results showed improvement of dielectric behavior with metal oxides loading. The experimental data of composite blended membranes can be used in optoelectronics devices.
The Colonial Clinic in Conflict: Towards a Medical History of the Palestinian Great Revolt, 1936–1939
Authors: Sandal-Wilson Chris
Published: March 2022

This article reconstructs how Arab doctors, medical missionaries, British counterinsurgents, and Palestinian rebels negotiated and contested the legitimate role of medical workers and healthcare in times of colonial conflict. Drawing insight from a medical anthropological literature which challenges the notion of medical neutrality as normative, and setting mandate Palestine alongside other case studies of medicine in times of conflict from the interwar Middle East and North Africa, this article argues that while healthcare and medical authority could be put to work to support the colonial status quo, they could serve other, more radical ends too. To highlight the complexity of the political positioning of medical workers and healthcare, this article focuses on the town of Hebron during the great revolt which rocked the foundations of British rule in Palestine between 1936 and 1939, and relies on a range of colonial and missionary archival sources. The first part of the article uses the case study of an Egyptian medical doctor who took up political office in the town in moments of crisis to show how medical authority could be consciously transmuted into a force to uphold a besieged political order. The second part draws on the diary of a British mission doctor to reconstruct his efforts to assert medical neutrality during the great revolt, and—more strikingly still—how Palestinian insurgents participated actively in this attempt to transplant international legal protections to Hebron. The final part traces the incorporation of healthcare into the strategies of both British counterinsurgents and Palestinian rebels, with the British policy of collective punishment indirectly but appreciably degrading access to healthcare for Palestinians, and Palestinian counterstate ambitions extending to the establishment of insurgent medical services in the hills.
PAMAM versus PEI complexation for siRNA delivery: interaction with model lipid membranes and cellular uptake
Authors: Chang Patrick K. C. , Prestidge Clive A. , Bremmell Kristen E.
Published: March 2022

Purpose

Cationic polymers have many advantages as vectors for mediated cellular entry and delivery of siRNA. However, toxicity related to their cationic charge has compromised clinical use. It is hypothesized that the siRNA-vector complex composition and properties can be controlled to optimize therapeutic performance. Here we investigate siRNA complexes with branched polyethylenimine (bPEI) versus generation 4 polyamidoamine dendrimers (PAMAM) on interactions with immobilized lipid membranes, and cellular uptake and toxicity.

Methods

A model siRNA was complexed with either PAMAM or bPEI, and their size and zeta-potential characterized. Interaction of the complexes and parent polymers with lipid bilayers was investigated using atomic force microscopy and correlated with the uptake and toxicity in HeLa cells.

Results

PAMAM and its siRNA complexes formed circular shaped micron-sized holes in lipid bilayers, while bPEI formed nanoscale holes. Flow cytometry and fluorescence microscopy demonstrated PAMAM-siRNA complexes to have a higher cellular uptake than bPEI-siRNA complexes. bPEI-siRNA complexes did not impact on viability, however PAMAM-siRNA complexes demonstrated increasing cell toxicity as N/P ratio increased. PAMAM-siRNA complexes accumulated around the cell nucleus, while PEI-siRNA complexes were located closer to the cell wall.

Conclusion

Complexation of PAMAM dendrimer or bPEI with siRNA modified physicochemical properties of the parent polymer, however it did not impact on the mechanism of interaction with model lipid bilayers or how the polymer/siRNA complex interacted and was internalized by HeLa cells. Interaction of siRNA polymer complexes with cells is related to the action of the parent polymer.

Graphical abstract
Hepatoprotective potential of selected medicinally important herbs: evidence from ethnomedicinal, toxicological and pharmacological evaluations
Authors: Arman Mohammad , Chowdhury Kazi Ashfak Ahmed , Bari Md. Sazzadul , Khan Mohammad Forhad , Huq Maryam Md. Ataul , Haque Md. Areeful , Capasso Raffaele
Published: March 2022

Medicinal herbs are nature’s gift to humanity, contributing crucially to the preservation, maintenance and improvement of our health. In order to explore the hepatoprotective potential of prevalent medicinal plants, nine species were selected from different genera based on their ethnomedicinal records in treating different liver-related pathological conditions in South Asia. Besides, the available information was critically analyzed to gain new insights and directions for future investigations towards establishing such natural products as potent hepatoprotective agents or dietary supplements. The hepatoprotective activities of the species have been investigated in a wide variety of in vivo experimental models including carbon tetrachloride-, paracetamol-, iron-, mercuric chloride-, thioacetamide-, cyclophosphamide-, β-d-galactosamine-, cumene hydroperoxide-, α-naphthyl-isothiocyanate-, physical stress- and alcohol-induced hepatotoxicity in rats and mice. All the plants were consistent in their ability to possess hepatoprotective properties. As well, three bioactive isolates namely, schaftoside, echinocystic acid, and eclalbasaponin II were found to have promising hepatoprotective potential. However, extensive comparative studies are warranted in future to establish the relative hepatoprotective potentials of the nine species under discussion. Preparation of poly-herbal formulations from these plants and identification of hepatoprotective phytoconstituents from these plants might open up new avenues in the development of therapeutic hepatoprotective agents.
Effect of post-harvest practices on greenhouse gas emissions in rice paddies: flooding regime and straw management
Authors: Belenguer-Manzanedo María , Alcaraz Carles , Camacho Antonio , Ibáñez Carles , Català-Forner Mar , Martínez-Eixarch Maite
Published: March 2022

Aims

To assess 1) the effect of the combination of flooding (winter flooding vs. non-winter flooding; WFL vs NWF) and timing of straw incorporation (early vs late straw incorporation; ESI vs LSI) in the post-harvest of paddy agrosystem, on a year-round global balance of greenhouse gases (GHG) exchanges, i.e. methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O); 2) the impact on the net ecosystem carbon balance (NECB) and 3) the resulting net global warming potential (GWP).

Methods

A field experiment was conducted with fortnightly samplings of main GHG emissions. Effect of the studied factors on GHG emissions was seasonally assessed. The net GWP is estimated from the balance between GHG (CH4 and N2O) and NECB.

Results

NWF-LSI reduced net GWP by 206% compared to conventional post-harvest management (WFL-ESI). NECB was similar in all treatments. Avoiding winter flooding reduced CH4 emissions significantly in the post-harvest and next growing seasons, while delay straw incorporation prevented CH4 and CO2 emissions during post-harvest. None of the treatments increased N2O emission. Environmental implications of post-harvest management options are discussed.

Conclusions

Post-harvest management affects net GWP of the paddy rice cultivation by modifying GHG emissions in post-harvest and next growing season without compromise sequestration C budget. The combination of non-winter flooding and late straw incorporation strategies were more effective in reducing both CH4 and CO2 emissions, due to avoiding higher temperatures at the time of the straw incorporation during post-harvest and increasing soil Eh conditions at the following growing season.
The role of microbes in the increase of organic phosphorus availability in the rhizosheath of cover crops
Authors: Hallama Moritz , Pekrun Carola , Mayer-Gruner Paula , Uksa Marie , Abdullaeva Yulduz , Pilz Stefan , Schloter Michael , Lambers Hans , Kandeler Ellen
Published: March 2022

Background and aims

The characterisation of plant-available phosphorus (P) pools and the assessment of the microbial community in the rhizosheath of cover crops can improve our understanding of plant–microbe interactions and P availability.

Methods

Mustard (Sinapis alba), phacelia (Phacelia tanacetifolia) and buckwheat (Fagopyrum esculentum) were grown as cover crops before soybean (Glycine max) in an on-farm experiment on a soil low in available P in southwest Germany. The cycling of P through the cover crop biomass and the enzyme-availability of organic P (Porg) pools in the cover crop rhizosheath were characterised. The soil microbial community (PLFA), activity (acid and alkaline phosphomonoesterase, as well as phosphodiesterase), and microbial P were assessed. The abundance of 16S-rRNA andphoD, coding for alkaline phosphomonoesterase in bacteria, were quantified using real-time qPCR.

Results

Mustard contained the greatest amount of P in its large biomass. In the rhizosheath of all cover crops, the concentration of enzyme-labile Porgwas higher than that in the control bulk soil, along with substantial increases of microbial abundance and activity. There were little differences among cover crop species, few changes in the bulk soil and only a limited carryover effect to soybean, except for fungi.

Conclusions

Turnover of microbial biomass, especially saprotrophic fungi, increased by rhizodeposition of cover crop roots; this was likely responsible for the observed increases in enzyme-available Porg. Microbial function was correlated linearly with microbial biomass, and the data of enzyme activity andphoDdid not suggest a difference of their specific activity between bulk and rhizosheath soil.
Particles and Photons as Drivers for Particle Release from the Surfaces of the Moon and Mercury
Authors: Wurz P. , Fatemi S. , Galli A. , Halekas J. , Harada Y. , Jäggi N. , Jasinski J. , Lammer H. , Lindsay S. , Nishino M. N. , Orlando T. M. , Raines J. M. , Scherf M. , Slavin J. , Vorburger A. , Winslow R.
Published: March 2022

The Moon and Mercury are airless bodies, thus they are directly exposed to the ambient plasma (ions and electrons), to photons mostly from the Sun from infrared range all the way to X-rays, and to meteoroid fluxes. Direct exposure to these exogenic sources has important consequences for the formation and evolution of planetary surfaces, including altering their chemical makeup and optical properties, and generating neutral gas exosphere. The formation of a thin atmosphere, more specifically a surface bound exosphere, the relevant physical processes for the particle release, particle loss, and the drivers behind these processes are discussed in this review.
Portable X-ray fluorescence (pXRF) analysis of heavy metal contamination in church graveyards with contrasting soil types
Authors: Madden Charles , Pringle Jamie K. , Jeffery Adam J. , Wisniewski Kristopher D. , Heaton Vivienne , Oliver Ian W. , Glanville Helen , Stimpson Ian G. , Dick Henry C. , Eeley Madeleine , Goodwin Jonathan
Published: March 2022

Human remains have been interred in burial grounds since historic times. Although the re-use of graveyards differs from one country, region or time period to another, over time, graveyard soil may become contaminated or enriched with heavy metal elements. This paper presents heavy metal element soil analysis from two UK church graveyard study sites with contrasting necrosols, but similar burial densities and known burial ages dating back to the sixteenth century and some possibly older than 1,000 years. Portable X-ray fluorescence element laboratory-based analyses were undertaken on surface and near-surface soil pellets. Results show elevated levels of Fe, Pb, Mn, Cr, Cu, Zn and Ca in both necrosols when compared with background values. Element concentration anomalies remained consistently higher than background samples down to 2 m, but reduced with distance away from church buildings. Element concentration anomalies are higher in the clay-rich necrosol than in sandy necrosol. Study result implications suggest that long-used necrosols are likely to be more contaminated with heavy metal elements than similar soil outside graveyards with implications for burial grounds management, adjacent populations and where burial grounds have been deconsecrated and turned to residential dwellings.
Low-precision feature selection on microarray data: an information theoretic approach
Authors: Morán-Fernández Laura , Bolón-Canedo Verónica , Alonso-Betanzos Amparo
Published: March 2022

The number of interconnected devices, such as personal wearables, cars, and smart-homes, surrounding us every day has recently increased. The Internet of Things devices monitor many processes, and have the capacity of using machine learning models for pattern recognition, and even making decisions, with the added advantage of diminishing network congestion by allowing computations near to the data sources. The main restriction is the low computation capacity of these devices. Thus, machine learning algorithms capable of maintaining accuracy while using mechanisms that exploit certain characteristics, such as low-precision versions, are needed. In this paper, low-precision mutual information-based feature selection algorithms are employed over DNA microarray datasets, showing that 16-bit and some times even 8-bit representations of these algorithms can be used without significant variations in the final classification results achieved.
Assessing the Levels of Functional Adaptation: Finite Element Analysis Reveals Species, Hybrid, and Sexual Variation in the Biomechanics of African Cichlid Mandibles
Authors: McWhinnie Kirsty , Gibson Jeremy , Gislason Magnus , Tanner Elizabeth , Windmill James , Albertson RCraig , Parsons Kevin
Published: March 2022

To understand how adaptive divergence emerges it is essential to examine the function of phenotypic traits along a continuum. For vertebrates, the mandible provides a key link with foraging and other important activities which has made it highly relevant for investigations of biomechanical change. Variation in mandible shape is known to correspond with ecology but its function is often only investigated between distinct species. However, for such divergence to occur and be maintained selection likely draws from many sources of biomechanical variation. African cichlids represent an exemplar model for understanding how such processes unfold with mandible variation existing between species, sexes, and is likely generated in nature by the potential for hybridization. We explored such mandible variation through a finite element modelling approach and predicted that hybrids and females would have reduced functional capabilities, the former in line with disruptive selection and the latter due to potential trade-offs incurred by maternal mouthbrooding in Malawian haplochromines. We revealed evidence of structural adaptations betweenTropheops‘Red Cheek’ andLabeotrophues fuellebornithat impacted the dispersion of mechanical stress in ways that matched the foraging of these species. Also, hybrids showed higher stresses relative to both species across the mandible. Sexual dimorphism in stress handling was evident despite minor differences in shape with males showing enhanced load resistance. However, in hybrids it appeared that males were disadvantaged relative to females, and displayed asymmetry in load handling. Together, these results show evidence of species and sex based biomechanical variation, that could be targeted by divergent selection.