bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparasi DNA Spesies Colletotrichum sp. dan Spesifitas Sistem Fingerprinting RAPD
Authors: Jamsari Jamsari
Published: December 2019

DNA preparation from C. capsici dan C. gleosporides is important for PCR based analysis of anthracnose causingpathogens. On the other hand, RAPD as one of DNA-based fingerprinting is hindered by its instability and its nonspecifity problems. For that reason, converting RAPD fragments into other DNA based systems is an alternative toincrease its analysis reliability. The study showed that mycelia grown in 2-days liquid culture is appropriate materialfor DNA preparation especially when combined with Shagai-Maroof protocol as well as Promega Genomic DNAIsolation Kit. The study indicated also that some isolated RAPD-fragments showed its instability character. Thiswas proved by the occurrence of multi different length of fragments after re-amplification of some single RAPDspecific fragments.
Pemarasitan Benalu Dendrophthoe pentandra (L.) Miq. pada Tanaman Koleksi Kebun Raya Cibodas, Jawa Barat
Authors: Sunaryo Sunaryo
Published: December 2019

The mistletoes of Dendrophthoe pentandra (Loranthaceae) grow as parasite on several plant collections of CibodasBotanical Gardens. Plant collections which attacked by mistletoe are Ficus type (Moraceae), and by individual innumber at most is Syzygium racemosum (Myrtaceae). 299 infected and uninfected branches of 67 host specieswere observed. The results showed that the host branches can be destroyed by mistletoe. Moreover, in the naturalconditions, the mistletoe causes degraded of the distal part of the infected branches.
Kowanin, Suatu Santon dari Kulit Batang Garcinia cowa Roxb
Authors: ' Darwati , Bahti Husen H , ' Supriyatna , ' Dachriyanus
Published: December 2019

The compound tetraoxygenated xanthone was isolated from the crude extract of the stem bark of Garcinia cowaRoxb. The compound tetraoxygenated xanthone was caried out as yellow crystal with melting point 136-1370C.The structure of this compound was detremined base on spectroscopic methods, including UV, IR, 1H-NMR,13C-NMR 1D and 2D. The compound was found to exhibit cytotoxicity against T47D cell by SRB method
Efek Beta Karoten dan Agregasi Klorofil Pada Fotostabilitas Klorofil a dalam Pelarut Aseton
Authors: Costa Junet. F. da , Karwur Ferry F , Limantara Leenawaty
Published: December 2019

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.
Aktivitas Promoter â-aktin Ikan Medaka Jepang (Oryzias latipes) pada Ikan Mas (Cyprinus carpio)
Authors: ' Alimuddin , Purwanti Lola Irma , Ath-thar MH. Fariduddin , Muluk Chairul , Carman Odang , Sumantadinata Komar
Published: December 2019

This study was conducted to examine activity of medaka (Oryzias latipes) â-actin promoter (mBP) in common carp(Cyprinus carpio) as the first step towards development of common carp transgenic in country. Gene constructpmBP-hrGFP that consists of mBA promoter and humanized Renilla reniformis green fluorescent protein gene(hrGFP) was injected into cytoplasm of one cell stage of common carp by using microinjector. PmBP-hrGFPconcentration used for microinjection was 50 μg/mL aquabides. Parameters observed were survival rate of embryo(SRe), hatching rate (HR) and expression of hrGFP gene. SRe was calculated before eggs hacthed, while hatchingrate (HR) was after all of eggs hatched. The activity of mBA promoter was analyzed by observation of hrGFP genetransient expression using a fluorescence microscope. The results of experiment showed that SRe (87,5%) andHR (79.2%) of control was respectevily higher than that of injected treatment (75.0% & 61.7%). Expression of hrGFPwas observed firstly at blastula (12 hours after fertilization) to 1-day-old larval stages (24 hours after hatching)with higher gene expression at blastula to late gastrula stages. Percentage of micronjected larvae expressinghrGFP at 6 hours after hatching reached 71.6 ± 6.7%. Conclusion was that mBA promoter could drove hrGFPexpression in common carp, hence it can be used to produce common carp transgenic by changing hrGFP withgenes correlated with important traits in aquaculture.
Fotoproteksi Kurkumin terhadap β-Karoten pada Berbagai Nisbah Molar serta Aktivitas Antioksidannya
Authors: Natalina Elly , Rahayu Puji , Sulistyowati Sulistyowati , Limantara Leenawaty
Published: December 2019

Curcumin and β-carotene are two kinds of pigments serve as antitumor, anticancer and antioxidant agents. Theantioxidant activity of curcumin is stronger and more stable as compared to β-carotene, so it can protect β-carotene degradation from light and oxygen. Therefore curcumin has higher antioxidant activity as well. Theresearch was conducted by mixing curcumin and β-carotene in several molarity ratios followed by iradiation usingvolpi lamp for 0, 10, 30, 60, and 90 minutes. The result shows that curcumin has photoprotection effect because itcan protect photooxidation of β-carotene. Mixing of curcumin and β-carotene 1:1 was the most stable ratio forprotecting β-carotene.
Pengaruh CdCl2 terhadap Produksi Eksopolisakarida dan Daya Hidup Azotobacter
Authors: Hindersah Reginawanti , Arief Dedeh Hudaya , Soemitro Soetijoso , Gunarto Lukman
Published: December 2019

The contamination of toxic heavy metal Cadmium (Cd) in soils will be endanger the human health because it ismore available comparing to another toxic heavy metals. One method of Cd-contaminated soil bioremediation isusing exopolysachharide-producing bacteria Azotobacter. Exopolysachharides (EPS) can mobilize Cd through theformation of complex Cd-EPS which sequentially can increase the availability of Cd for plants uptake. A laboratoryexperiment has been done to study the EPS production and the viability of six Azotobacter isolates in the liquidculture containing 0.01, 0.1, and 1 mM CdCl2. The bacteria were cultured in liquid medium with and without CdCl2 for72 hours at room temperature. The EPS production was determined by gravimetric method after precipitationusing acetone and centrifugation at 7000 rpm. The result was that all of Azotobacter isolates produce EPS in thepresence of CdCl2. In the culture with 1 mM CdCl2, the density of Azotobacter sp. isolate BS3, LK5, LKM6 increasedsignificantly, and that of isolate LH16 decreased. No significant effect of CdCl2 on the density of isolate BS2 andLH15. This research suggested that some Azotobacter isolates were relatively resistence to the Cd and could bedeveloped as biological agents in Cd-contaminated soil bioremediation.
Sintesis dan Aplikasi Karboksimetil Kitosan sebagai Inhibitor Korosi pada Baja Karbon dalam Air
Authors: Erna Maria , Emriadi Emriadi , Alif Admin , Arief Syukri , Noordin Mohd Jain
Published: December 2019

Carboxymethyl chitosan (CMC) was synthesized with different methods by reacting chitosan with monochloroaceticacid in the presence of sodium hydroxide under variation conditions. The above samples were characterized byFourier transform infrared spectroscopy (FTIR) and soluble in water in range of pH. The CMc were soluble in watera wide range of pH and applied as corrosion inhibitor for steel in water. The inhibiting influence of CMC was studiedby potentiodynamic polarization method. It was found thad corrosion rate was dependent on water pH and CMCconcentration. The results show that optimum the inhibition efficiency at pH 5 and 1 ppm concentration CMC, i.e.,77%. The adsorption of used compound on the steel surface obeys modified Langmuir isotherm. Polarizationmeasurement show that the CMC acts essentially as a anodic-type inhibitor.
Perilaku Disolusi Ketoprofen Tersalut Gel Kitosan-Karboksimetilselulosa (CMC)
Authors: Sugita Purwantiningsih , Achmadi Suminar Setiati , Yundhana Yuyu
Published: December 2019

Study dissolution behaviour of ketoprofen through optimum chitosan-CMC microcapsule has been carried out. Into228.6 ml of 1.0% (w/v) chitosan solution in 1% (v/v) acetic acid, 38.1 ml of CMC solution was added with concentrationvariation of 0.075; 0.0875; and 0.10% (w/v). Afterwards, 7.62 mL of glu was added slowly under stirring, withconcentrations varied: 3; 4.5; and 6% (v/v). All mixtures were shaked for 20 minutes for homogenization. Into eachmicrocapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added. Everymixture was then added with 5 ml of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature.Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. Thedissolution behaviour of optimum ketoprofen microcapsules were investigated in gastric and intestinal medium.Microcapsule morphology before and after dissolution as well as empty microcapsule (blank) were observed withSEM. Spray drying process had successfully coated ketoprofen in chitosan-CMC microcapsule. Optimization byusing Minitab Release 14 software showed that among the microcapsule compositions studied, CMC and glu of0.0925% (w/v) and 3.01% (v/v), respectively, optimum to coat ketoprofen at constant chitosan concentration 1.0%(w/v). Result of SEM morphology and In vitro dissolution profile showed that ketoprofen in chitosan-CMCmicrocapsule was relatively well than chitosan-guar gum microcapsule. Kinetically, dissolution of ketoprofen frommicrocapsule in intestinal pH condition was first order with release rate constant, k, of 7.285  10-4 % min-1 andrelease half-time, t1/2, of approximately 15 hours.
Pembuatan Biodiesel dari Minyak Kelapa melalui Reaksi Metanolisis Menggunakan Katalis CaCO3 yang dipijarkan
Authors: Padil Padil , Wahyuningsih Slamet , Awaluddin Amir
Published: December 2019

Biodiesel is one of alternative fuels. Biodiesel can be made from coconut oil and is referred to as cocodiesel. Thisresearch studies the optimum condition of cocodiesel production by methanolysis reaction between coconut oiland methanol using heterogen catalyst of calcium carbonate (CaCO 3) is calcined for 1.5 hours at 900oC. In order toget optimum condition, several experimental parameters are applied such as catalyst concentration 1-3 %-wt andmolar ratio of methanol/coconut oil 4:1-12:1. The optimum condition obtained from this experimental as follow:catalyst concentration was 2%, molar ratio of methanol/coconut oil was 8:1 at 600C, produced the higgest conversionof cocodiesel 75.02%. The cocodiesel meet the requirement of Standar Nasional Indonesia (SNI) specifications.Based on data analysis, the product has a qualification as diesel fuel. SNI Biodisel is density (40 0C) 850-890Kg/m3,kinematic viscosity (40 0C) 2,3-6,0 mm2/s, cetane number min 51, iod number max 115 gr iod/100 gram,moisture content max 0,05 % volum, acid number max 0,8 mg KOH/g, flash point min 100 0C.
Karakter Root Re-Growth Sebagai Parameter Toleransi Aluminium pada Tanaman Padi
Authors: Roslim Dewi Indriyani , Miftahudin Miftahudin , Suharsono Utut , Aswidinnoor Hajrial , Hartana Alex
Published: December 2019

Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can beselected from plant breeding program by one of the physiological parameters representing Al tolerance character,such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentrationand time exposure of Al stress that was able to differentiate the response of three local upland rice varieties(Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectivenessof root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments,which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) potexperiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 asplanting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitivevarieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrientculture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. Thesevariations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can beeffectively applied to screen rice F2 population that segregate to Al tolerance character.
Biodisel dari Minyak Nyamplung (Callophyllum inophyllum) dan Spiritus dengan Katalisator Kapur Tohor
Authors: Marnoto Tjukup , Sulistyawati Endang
Published: December 2019

Production of biodiesel has been done by many institutions; however the materials used are usually competedwith food such as palm oil, therefore it has lesser economical value. The use of Callophyllum inophyllum plant inIndonesia is very limited and the seeds are usually thrown away the contain high oil relatively. This plant is relativelyeasy to grow and has seasonal fruiting mostly planted as shading plant in rural area and also as land slide resistingplant. The plant is normally utilized for light construction and burning wood. The technology for producing biodieselis simple and can be easily introduced to the society. Biodiesel produced from easily obtained and low costmaterials will improve a society’s energy independency and well-being. This paper presents the process of producingbiodiesel from Callophyllum inophyllum oil and rubbing alcohol using superficial lime (CaO) as the solid catalystthat is cheap and easily obtained, therefore this technology can be easily transferred to the society and willimprove the economical value of the plant. Referring to the varieties used, relatively good condition was at 1:3 molratio of Callophyllum inophyllum oil to rubbing alcohol and the amount of catalyst used was 0.3-0.5% of the rubbingalcohol. Physical characteristics of the produced biodisel were such as specific gravity at 60/600F was 0.870, flashpoint P.M.cc was 1950C and pour point was 40C.
Senyawa Antimalaria dari Jamur Endofitik Tumbuhan Sambiloto (Andographis paniculata Nees)
Authors: Elfita Elfita , Muharni Muharni , Munawar Munawar , Salni Salni , Oktasari Ade
Published: December 2019

Plants have been the chief source of compounds of medicine for thousand of years. Plants are also the source ofmany medicines for the majority of the world’s population. The role of biotechnology is very important for multiplying,conserving the spesies, and enhancing the production of secondary metabolites. Endophytic are microbes thatinhabit plants are currently considered to be a wellspring of novel secondary metabolites offering the potensial formedical and industrial exploitation. Plants with ethnobotanical history, for example sambiloto (Andographispaniculata Nees) are likely candidates for finding bioactive compounds. Isolation begin with cultivation of Aspergillusflavus fungi in 2 liter of Potato Dextrose Broth media for four weeks. Media is extracted into the solvent n-hexaneand ethylacetate following by evaporation. Ethylacetate extracts were separated by chromatography techniquesin order to get pure compound in the form of white crystal. Phytochemical tests showed that the isolated compoundis alkaloid. The molecular structure of the isolated compound was determined based on spectroscopic data,including UV, IR, 1H-NMR, 13C-NMR, HMQC, and HMBC spectrum. The compound was determined as7-hydroxypiranopiridin-4-on with molecule formula C8H7NO2 (Mr=149). The compound has antimalarial activityagainst Plasmodium falciparum 3D7, with IC50 values 0,201 μM.
Efek Sumber Karbon Berbeda terhadap Produksi â-Glukan oleh Saccharomyces Cerevisiae pada Fermentor Air Lift
Authors: Kusmiati Kusmiati , Thontowi Ahmad , Nuswantara Sukma
Published: December 2019

The need of â-glucan is increasing in food, medicine and cosmetic industry, because it becomes anticancer,antitumor and antiaging, increases immunosystem, and decreases cholesterol content in blood. The cell walls ofS. cerevisiae contain 80-90% polysaccharides that posses â-glucan. This research was aimed to obtain appropriatecarbon sources to increase the production of â-glucan. The carbon sources used were glucose, glucose commercial,sucrose and molases. The fermentation process was done by using air lift fermentor. The steps of fermentatonincluded regeneration of S. cerevisiae strain, preculture, fermentor preparation and running fermentor for 84hours. Sampling of S. cerevisiae culture was determined the cell growth by optical density (OD) usingspectrophotometer UV/VIS at ë 550 nm. The protein content was determined by Lowry method at ë 755 nm and thetotal glucose was measured by phenol sulphate method at ë 490 nm. The measurement result of cell growthshowed that the high intensity of S. cerevisiae in medium contain molases, but it did not show significant effectwhen compare to other carbon sources. The protein and carbohydrate contain in medium tended to decrease. Theresult of â-glucan on glucose, sucrose, glucose commercial and molases were 933,3, 1100, 1000, and966,7 mg/l. It can be concluded that sucrose and glucose commercial can replace the glucose to produce of â-glucan, because they are cheaper and easier to get. Beside that, molases can be used as an alternative carbonsource because it can produce of â-glucan as well as glucose.
Seleksi, Karakterisasi, dan Identifikasi Bakteri Penghasil Kitinase yang Diisolasi dari Gunung Bromo Jawa Timur
Authors: Soeka Yati Sudaryati , Sulistiani Sulistiani
Published: December 2019

Selection, characterization and identification of bacteria that can produce chitinase enzyme were isolated fromBromo Mountain, East Java. The 48 isolates were tested for capability to degrade chitine qualitatively, semiquantitatively and quantitatively. The result showed that 2 isolates, B2-4 and NA S4-1 could degrade chitin, withactivities of 4.8. 10-3 and 3.1 . 10-3 U/ml, after 1 and 2 days incubation respectively. By using molecular characterizationmethods, partial sequences of 16S rDNA and the primers 9F & 1510R were identified as Stenotrophomonas sp.
Pengaruh Likopen terhadap Penurunan Aktivitas Mitogen-Activated Protein Kinase (MAPK) dan Ekspresi Endothelin-1 (ET-1) pada Kultur Huvecs yang Dipapar Leptin
Authors: Fatmawati Heni , Satuman Satuman , Rudijanto Ahmad , Indra Muhammad Rasjad
Published: December 2019

The effect of obesity on vascular function is mediated by hormon leptin. Leptin has been proved to increaseoxidative stress in endothelial cell. The previous study has proven that leptin caused the endothelial dysfunction asa step of the atherogenesis. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesismechanism, which is stimulated a proinflamatory cytokine and adhesion molecules by MAPK and transcriptionfactor ET-1. Therefore, the aim of this research was to prove and to determine whether lycopene could decreasethe MAPK and ET-1 expression in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/mlleptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/ml leptinand 0 ìM lycopene, (2) induced by 500 ng/ml leptin for 12 hours, (3) induced by leptin and lycopene with concentration10; 25; 40; 55 and 75 ìM for 12 hours. Then the identification of MAPK was applied by using imunocytochemistrycompared with ELISA procedure on cell endothel culture lysate and ET-1 expression was measured by using RTPCR. It was showed that lycopene 10-25 ìM decreased MAPK and ET-1 expression significantly in HUVECs cultureinduced by leptin 500 ng/ml. Leptin was increased ERK1/2 MAPK and ET-1 expression in HUVECs culture and candecrease by lycopene. Optimum dose of lycopene is 10-25 ìM.
Kongruensi Unsur Idempoten Ortogonal dalam Aljabar Insidensi Finitary
Authors: Carnia Ema , Wahyuni Sri , Irawati Irawati , Setiadji Setiadji
Published: December 2019

Let X be a partially ordered set, R is a commutative ring with identity and FININC (X, R) denote finitary incidencealgebra of poset X over R. In this paper it will be seen congruence of two elements that are idempotent orthogonalin FININC (X, R) relative to the modulo Radical Jacobson of algebra. Review of this topic would be useful to examineisomorphism problems of the finitary incidence Algebra.
The Genus Licuala Wurmb (Arecaceae) in Java
Authors: Siregar Etti Sartina , Tjitrosoedirdjo Sri Sudarmiyati
Published: December 2019

The comprehensive revision of the genus has been done in the Malay Peninsula, New Guinea, Sumatra andKalimantan. In Java, the revision of Licuala has never been recently completed. This study was based onmorphological characters of specimens which are preserved in Herbarium Bogoriense. The several living collectionscultivated in Bogor Botanical Garden were studied. The aim of this study is to ascertain the correct names andimprove the species delimitation of Licuala in Java. The result shows that there are three species of Licuala inJava. They are L. gracilis, L. pumila and L. spinosa. Two previously known species have been placed into synonymies;L. flabellum to L. gracilis, and L. spectabilis to L. spinosa. Licuala gracilis is endemic for west Java.
Pengaruh Suhu terhadap Perkembangan Pradewasa Parasitoid Eriborus argenteopilosus Cameron (Hymenoptera: Ichneumonidae)
Authors: Nelly Novri , Habazar Trimurti , Syahni Rahmat , Buchori Damayanti
Published: December 2019

Temperature effect on development time of the preadult parasitoid Eriborus argenteopilosus Cameron(Hymenoptera: Ichneumonidae) were studied to know development time, degree days and survival rate.Crocidolomia pavonana (Fabricius)(Lepidoptera: Pyralidae) larvae was exposed to E. argenteopilosus female andreared at four different temperatures i.e 160C, 200C, 250C and 300C. Data were analyzed using anova and linearregression to calculate degree day. At 200C E. argenteopilosus showed the highest degree day and survival rate(18.67 %), while at 300C nothing adult parasitoid emergenced. Degree day to development time of parasitoid attemperature 200C i.e fase egg-adult: 300.05; egg-pupae 173.35; pupae-adult 171.
Adsorption Isotherms of Phenol Onto Adsorbents Derived from Egg Shell and Palm-Oil Shell
Authors: Asyhar Rayandra
Published: December 2019

The adsorption isotherms of phenol from aqueous solution onto adsorbents obtained from egg shell (ESA) andpalm shell (PSA) were investigated. The objectives of the investigation were to understand the effect of bothadsorbents on solution pH and to study the adsorption equilibrium of phenol onto the adsorbents. The effect ofadsorbent on pH of solution was studied by shaking the adsorbent of 0.1 to 1.5 g with 100 ml of acidic aqueoussolution for 30 min at room temperature. The adsorption experiments were performed by stirring appropriateamount of adsorbent with 100 ml of 50 mg/l concentration of phenol at constant temperature and pressure. TheLangmuir and Fraundlich adsorption models were applied to experimental data and the isotherm constants werecalculated using linier regression analysis. The results showed that the adsorption capacity of the adsorbentsincreases with increasing of dosage and contact time. Also, pH of solution affected the adsorption isotherm ofphenol, where maximum adsorption was observed at pH values lower than 9.
Kajian Awal Pemanfaatan Rumput Teki (Fimbristylis sp), Linggi (Penicum sp) dan Sianik (Carex sp) sebagai Serat Alami untuk Bahan Alat Penangkapan Ikan
Authors: Nofrizal Nofrizal , Ahmad Muchtar , Syofyan Irwandy , Habibie Ied
Published: December 2019

This research was aimed to study about the potential of fiber structure teki grass (Fimbristylis sp), linggi grass (Penicum sp) and sianik grass(Carex sp) as natural fibre for fishing gear materials. The parameter of this research are; water content, elongation, breaking strength andhistology of the fiber. This research used the observation and experimental method. The observation method is used to know the fiberstructure and water content of teki grass, linggi grass and sianik grass. The experimental method was used to test the breaking strength andelongation of the objects in wet and dry condition. Based on the histology observation, teki grass has an epidermis layers as fiber structure.It consists of parenchyma tissue and collenchymas tissue.Fiber structure of linggi grass and sianik grass are similar with teki grass. Sianikgrass has the most excessive epidermis layer than others i.e. 5.57 gram. The average water content of teki grass is 1.03 gram, and linggi grassis 0.17 gram. Based on the breaking strength test, sianik grass is stronger than others with average strength 9.05 kgf in wet condition and9.75 kgf in dry condition. Meanwhile, breaking strength of the teki grass and linggi grass is 3.20 kgf and 4.16 kgf in wet condition and 4.30kgf and 5.25 kgf in dry condition. The average of the elongation of sianik grass is longer than others i.e. 24.50 mm in wet condition and18.75 mm in dry condition. The elongation of teki grass is 9.05 mm in wet condition and 11.10 in dry condition. The average of linggi grasselongation is 12.20 mm in wet condition and 12.90 mm in dry condition. Based on the breaking strength and the elongation test, the resultshowed that sianik grass is the most potential natural fiber as fishing gear material.
Efek Pembukaan Lahan terhadap Karakteristik Biofisik Gambut pada Perkebunan Kelapa Sawit di Kabupaten Bengkalis
Authors: Suwondo Suwondo , Sabiham Supiandi , Sumardjo Sumardjo , Paramudya Bambang
Published: December 2019

Oil palm plantations at peatland are faced with problems of land degraded, low productivity and produced to green house gas (GRG) emission.The objectives of this study were to land clearing effect of peatlands and to identified factors that affect the peatlands in the biophysicalcharacteristics. This study was conducted on District Bengkalis-Riau at marine and brakish peat. The data was collected using field survey anddocumentation. The descriptive analysis was used to determine biophysical characteristics and linier regresion was used to corelation performimportant factors. The research results showed that the biophysical characteristics of peatland experienced changes in the horizon profile,peat thickness, decomposition level, moisture content, ash content, pH, C-organic and biomass. The secondary peat swamp forest ofbiophysical characteristics have to different in the horizon profile, peat thickness, decomposition level, moisture content, ash content, pH,C-organic and biomass compared with marine and brackish peat.
Pengaruh Air Perasan Umbi Bawang Merah (Allium ascalonicum L.) terhadap Malondialdehid (MDA) Plasma Mencit yang diinduksi Hiperkolesterolemia
Authors: Ismawati Ismawati , Asni Enikarmila , Hamidy M Yulis
Published: December 2019

Malondialdehyde, a product of lipid peroxidation, is often used as an indicator to detect the oxidative stress damaged in the body. Lipidperoxidation is associated with progression of atherosclerosis. It is known that onion (Allium ascalonicum L.) can be used as an antioxidantand hypolipidemic agent, but whether onion can decrease hypercolesterolemia mice MDA plasma induced by yolk is still unknown. Thepurpose of this study was to investigate the effect of onion’s juice on hypercolesterolemia mice MDA plasma level. This was an experimentallaboratory research with post test only design with control. The measured parameters were cholesterol and MDA level on tested groups.Groups with treatment consist of yolks group, and 80% shallot juice, simvastatin and aquadest were separately used as positive and negativecontrol. The result showed that there was significant difference between high fat feed group which added 80% onion’s juice and high fat feedgroup only. Addition of 80% onion’s juice in high fat feed groups decreased MDA plasma level equal to normal feed group. In conclusion, itwas proved that onion’s juice decreased mice MDA plasma level.
Identifikasi Komponen Kimia Damar Mata Kucing (Shorea Javanica) dengan Metode Pirolisis-GC/MS
Authors: Mulyono Noryawati , Wijaya Christofora Hanny , Fardiaz Dedi , Rahayu Wuryaningsih Sri
Published: December 2019

The aim of this research was to identify chemical compounds in cat eye dammar. The method included functional groups characterizationby infrared spectrophotometer and identification using Pyrolisis-GC/MS. Infrared spectra of crude sample showed that there were somefunctional groups such as alkyl, carbonyl, vinyl, and hydroxyl. Identification by Pyrolisis-GC/MS showed that dammar consisted of at least67 compounds. This natural gum components could be categorized into 4 groups, i.e. tetra cyclic hydrocarbon (30 compounds, 49.57%),penta cyclic (3 compounds, 2.56%), C 15 compounds (11 compounds, 17.09%), and other group (23 compounds, 18.26%). According to thePy-GC/MS data, brassicasterol is the highest relative concentration in dammar, i.e. 20%.
Proliferasi Kalus dari Eksplan Hipokotil dan Kotiledon Tanaman Jarak Pagar (Jatropha curcas L.) pada Pemberian 2,4-D
Authors: Zulkarnain Zulkarnain , Lizawati Lizawati
Published: December 2019

The aim of this study was to develop an efficient method for the induction of embryogenic callus formation for in vitro propagation ofjatropha. Plant materials used were 30-days old in vitro seedlings, cut into hypocotyl and cotyledon (lower, middle and upper) sections.Medium used was MS composition supplemented with vitamins, 3% sucrose, 0.7% agar at pH 5.8 ± 1, and 2,4-D (0, 1, 2, 3, 4 dan5 mg l-1). Cultures were kept at temperature of 25 ± 1 0C with 50 μmol m-2 s-1 light intensity and 16-h photoperiod. The results indicated thatthe rate of callus formation depended on the source of explant, the application of 2,4-D, and the interaction of both. The fastest callusproliferation (2.33 days following initiation) was obtained on cotyledon explants cultured on medium without 2,4-D. The explant sourcesand 2,4-D concentrations also showed significant effect on the percentage of explant forming callus. The most callus formation (88.33%)was obtained on middle cotyledon cultured on 3 mg l-1 2,4-D, whereas the fewest (6.84%) was found on upper cotyledon cultured on mediumwithout 2,4-D. The colour of callus was dominated by white, light yellow, cream and brown with mostly compact structure, particularly onhypocotyl cultured on medium without 2,4-D. The texture of callus formed on hypocotyl treated with up to 4 mg l -1 2,4-D was dominatedby coarse appearance. In contrast, majority of callus proliferated on hypocotyl treated with 5 mg l -1 2,4-D or cotyledon treated with orwithout 2,4-D produced callus with smooth texture %.
Perbanyakan Tanaman Pulai Pandak (Rauwolfia serpentina L.) dengan Teknik Kultur Jaringan
Authors: Yunita Rossa , Endang Endang , Lestarai Gati
Published: December 2019

Due to over exploitation of its bark for medicinal herbs and made worse by problem in conventional breeding, Rauwolfia serpentina (Pulaipandak), has been considered rare and was currently reported to be an endangered species. Therefore, conservation measure is urgent to betaken. One of them is by in vitro propagation. In this research, in vitro propagation covers several activities, such as (1) shoot inductionwith the application of MS (Murashige and skoog) media enriched with ZPT 0.0; 0.1; 0.3 mg/l BAP combined with 0, 1, 2 mg/l 2ip, (2)shoot multiplication by using 0.0; 0.5; 1.0 mg/l BAP combined with 0.0; 0.1; 0.2 and 0.3 mg/l thidiazuron), (3) root induction IBA at theconcentration of 0.0; 0.5; 1.0; 1.5; 2.0; 2.5; 3.0 mg/l, and (4) acclimatization. The result showed that the best shoot induction for calli isthe in vitro stem by the application of MS + 0.3 mg/l BAP + 1 mg/l 2ip basic media. For shoot multiplication, the best media was MS + 0.5mg/l BAP + 0.1 mg/l ; while the best formula for root induction was MS + 1 mg/l IBA. The best media for plantlet acclimatization is compost+ soil mixture in 1:1 ratio.
Pewarisan Sifat Densitas Stomata dan Laju Kehilangan Air Daun (rate leaf water loss RWL) pada Kacang Tanah (Arachis hypogaea L.)
Authors: Adisyahputra Adisyahputra , Sudarsono Sudarsono , Setiawan Kukuh
Published: December 2019

The aim of this research is to analyze and examine the inheritance of stomatal density trait and RWL as a variable in drought tolerance ofpeanut. The experiment was conducted by using cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerantgenotype as male parent, including population off spring from hybrid cv. Kelinci (P1) with US 605 (P2). Stomatal density was determinedby making leaf imprint and by observing leaf imprint under microscope. Relative water loss was determined by dipping peanut leaf in PEG40% for 48 hours. Result of the analysis showed that stomatal density and RWL were not only controlled by qualitative characters of majorgene, but also controlled by quantitative character of minor gene by polygenic with the complex gene action. Both characters seem toinfluence more as genetic factor and have high level fixation additive varians which can give the opportunity to obtain the tolerant offspring.
Desain dan Analisis Pengukuran Viskositas dengan Metode Bola Jatuh Berbasis Sensor Optocoupler dan Sistem Akuisisinya pada Komputer
Authors: Warsito Warsito , Suciyati Sri Wahyu , Isworo Dyan
Published: December 2019

It has been designed and analyzed the low cost viscometer using falling ball method, the sample analyzed is glycerin fluid at 20oC. Two optocoupler circuits have been used as time measurement system of falling ball between two references point, light source used was infrared laser diode. The computer acquisition system use serial communication and it has been perfectly made and characterized. The velocity measurement system has 0.75 x 10-1 s resolution, but the resolution of integrated system both of hardware and acquisition software, is about 10-1 s. The theoretical viscosity value has been calculated and simulated to abtain the absolute viscosity value. This simulation results have been analyzed and compared with the experiment results. The correction factor for velocity calculation has been discussed and gave the optimum value of velocity correction factor (0.4425), regarding to the dimension of tube and ball used in this research. Based on the experiment, the viscosity of glycerin obtained was 1418.0309±1.6157 mPa.s., this value was with similar with the literatures and has a 0.57% of accuracy error (ε0).
Profil Plumbum (Pb) dan Cadmium (Cd) sebagai Kontaminan Dampak Penggunaan Agrokimia serta Remediasi Biji Kedelai Menggunakan Swelling Agent pada Khelasi dengan Asam Sitrat
Authors: Priyadi Sapto , Darmaji Purnama , Santoso Umar , Hastuti Pudji
Published: December 2019

This research were aimed to know the plumbum (Pb) and cadmium (Cd) profile on soybean seeds as contaminants of the impact use of agrochemistry material impact and to know of swelling agent of N,N-dimethylformamide (DMF) needs at certain level of pH in chelation with citric acid. The results showed that the content of Pb in soybean seeds at cultivation system agrochemistry materials i.e. 0.63 ppm, while cultivation system without agrochemistry materials i.e. 0.46 ppm. Cd content in soybean seeds at cultivation system agrochemistry materials i.e. 0.05 ppm and the cultivation system without agrochemistry materials i.e. 0.1 ppm. Acceptable daily intake in terms of Pb content in soybean after treatment increases to 2.93 times compared before treatment (11.3379 to 33.2099 g per body weight per day). Acceptable daily intake in terms of the content of the Cd in soybean after treatment increases to 5.26 times compared before treatment. The value of the bio-concentration factor which expressed an accumulation of Pb in soybean seeds on both cultivation is low system (BCF<250), while for Cd is a moderate to high level (1.000250). Cd reduction on soybeans seeds for all chelation treatment was maximum (not detected-detection limits was 0.01 ppm), while the highest reduction of Pb occurs at swelling agent N, N-dimethylformamide 15 mL at pH 10.
Sintesis Tiga Peptida Bergugus Pelindung sebagai Prekursor Komponen Vaksin Influenza Universal
Authors: Subroto Toto , Hardianto Ari , Kahari Abdul Alim , Pradnjaparamita Tika
Published: December 2019

Current highly effective conventional vaccine to halt the spread of bird flu has not been invented yet because of susceptiblemutation of influenza virus. In spite of undergoing mutation which causes the amino acid sequence change, influenzaviruses maintain conservation at ectodomain of M2 protein, especially M2e(2-16) (SLLTEVETPIRNEW). The use ofconserved epitope M2e(2-16) in epitope-based vaccine potentially produces universal influenza vaccine. In designingepitope-based vaccine, the M2e(2-16) needs to be coupled with T helper epitope, P25, which is subsequently mentioned asM2e(2-16)-K-P25 (SLLTEVETPIRNEWGKKKL IPNASLIENCTKAEL). The M2e(2-16)-K-P25 was synthesized usingconvergent solid phase peptide synthesis strategy because of the size of the sequence. In this strategy, four peptideprecursors of M2e(2-16)-K-P25; SLLTEVETP (F1), IRNEWGK (F2), KLIPNASLI (F3), and ENCTKAEL (F4); were synthesizedin advance. After the precursors ready, coupling reaction was performed to obtain M2e(2-16)-K-P25. In the previousresearch, F3 has been obtained in high purity through Fmoc/tBu solid phase peptide synthesis method. In this conductedresearch, the three remaining precursors; F1, F2, and F4; were synthesized by the same method. Each peptide was analysedby thin layer chromatography, HPLC, and mass spectroscopy methods. F1, F2 and F4 were successfully synthesized andeach of them was detected at 1490.0, 1874.8 and 1881.9 amu, respectively. However, F1 was not possible to purify becauseof its insolubility in various solvents.
Kajian Kondisi Terumbu Karang dan Strategi Pengelolaannya di Pulau Panjang, Air Bangis, Kabupaten Pasaman Barat
Authors: Oktarina Angreini , Kamal Eni , Suparno Suparno
Published: December 2019

Coral reef ecosystems are essential for the survival of marine life, but the presence of coral reef are declined and decayed.Methods used in this study was a survey method. To retrieve the condition of coral cover using the line intercept transectwhile to determine management strategies using SWOT analysis. The average percentage of coral cover in 8 point researchstations at 50.10% are still in the good condition, which is in the north 3 m depth 53.51%, north 7 m depth 68.14%, east 3 mdepth 93.29%, east 7 m depth 15.93%, south 3 m depth 75.17%, south 7 m depth 20.53%, west 3 m depth 41.50%, and west7 m depth 32.79%. The strategies for coral reef ecosystem management, among others: 1) community-based management, 2)forming Kelompok Masyarakat Pengawas (Pokmaswas) institutions, 3) establish in Panjang Island as a marine conservationareas, 4) Ko-Management-based, 5) effort to improve the economy in order to support the increased knowledge of theimportance of conservation and management of coral reef ecosystem.
Distribusi dan Kemelimpahan Spesies Tumbuhan Bawah pada Naungan Pinus mercusii,Acacia auriculiformis danEucalyptus alba di Hutan Gama Giri Mandiri, Yogyakarta
Authors: Nahdi Maizer Said , Darsikin Darsikin
Published: December 2019

The purpose of this research was to study distribution and abundance of (shrubs, herbs, and rumput) under the three stands,i.e. Pinus mercusii, Acacia auriculiformis and Eucalyptus alba and environmental factors influencing them in Gama Girimandiri Forest. Data were collected in April-May 2011, using square plots. Plots were located in three locations by followinga stratified random sampling method. We administered 10 repetitions, and plot size 5x5 m for shrubs and herbs, and 1X1 m forgrass. The species found in each stand were identified and calculated for their density, frequency, domination, importantvalue, diversity index, and those parameters’ correlation with the measured environmental factors. The study shows that 59species of herbs and shrubs, and 12 species of grass. In the three stands, it could be shown various domination of grass,meanwhile there is no dominant species at the level of herbs and shrubs. The highest density, measured by diversity index,was observed at the stand of Pinus (3,13), followed by Acacia (3,12) and Eucalyptus (2,8). The distribution and density ofMemecylon sp and Oplismenus burmanni were observed at their highest value under the stand of Pinus, Lantana camaraLinn (37.2). At the same fashion, Paspalum conjugatum (58.3) does under the stand of Acacia, and Desmodium triflorum(64,2) and Ischaemum magnum (122) under Eucalyptus. Lantana camara could always be found under the three stands, ithad high important value, categorized as strategy species and invasive. Light density and pH significantly influenced thediversity index of shrubs and herbs. In addition, only soil temperature gave an effect to grass.
Respon Histopatologis Hepar Mencit (Mus musculus) yang Diinduksi Benzo(α)Piren terhadap Pemberian Taurin dan Ekstrak Daun Sirsak (Annona muricata)
Authors: Agata Annisa , Widiastuti Endang Linirin , Susanto G. Nugroho , ' Sutyarso
Published: December 2019

Cancer is a disease that is characterized by the existence of damage and cell abnormality in growth and differentiation. Liver cancer is a disorder of hepar tissue derivated from its tumors. Taurine is known as antioxidant but its role as anticancer needs to be explored more as well the role of Annona muricata leaf extract which is believed to have its role as anticancer substance. This research, therefore, aimed to explore the effect of taurine and Annona muricata leaf extract on the hepar histopathology of male mice (Mus musculus) induced by benzo(α)pyren in vivo. This research was carried out by using a complete randomized design, which consisted of 5 treatment groups which was repeated 5 times. Group I was given 0.2 mL corn oil for 15 days, group II was induced by benzo(α)pyren without taurin nor A. Muricata leaf extract for 10 days, group III was given 7.8 mg taurine/BW/day (twice a day) starting from the 15 th days before the induction of benzo(α)pyren, group IV, after induced with benzo(α)pyren, taurine was given with dosage of 7.8 mg/BW/day, group V, after induced with benzo(α)pyren, soursop leaf extract was given with amount of 277.8 mg/BW/day. Data analyzed by Kruskal-Wallis test and one way ANOVA with Fisher test (p>0.05). The results indicated that taurine had ability to recover the liver tissue induced by benzo(α)pyren as (carcinogenic) while, Annona muricata leaf extract had not shown any recover of tissue damage
Perception of Stigma among Caregivers of Mentally Ill People
Authors: Pandey Srijana
Published: December 2019

The lives of people with mental illness are altered by the symptoms of the illness, attitudes of people that view symptoms as threatening and uncomfortable, which foster stigma and discrimination within social circles. The catch of the study was to assess the stigmatizing beliefs among Caregivers of persons with mental illness. A descriptive and exploratory study was conducted with purposive sampling technique among 50 caregivers with interview method of mental illness patient with purposive sampling technique at Nepal Medical College teaching Hospital, Jorpati, Kathmandu, Nepal in Oct 2017- Nov 2017. The study revealed that the most of caregivers (62%) feel burdened financially, and about 64% think that people with mental illness are dangerous. Most of the caregivers (70%) think that the mentally ill people are violent. More than half (54%) of the caregivers think voluntary organizations are not doing adequate work in the field of mental health and 72%feel health organizations are giving less priority to mental illness as compared to other disorder. Most of the caregivers (66%) think that their occupational life has been interfered by the family member’s illness, and 56% don’t think that there are adequate law or rules regarding mental illness. In conclusion, care givers experience stigma such as concealment, negative experience, emotional stigma as well as work related stigma which affects the health and recovery of mentally ill persons. Compliance to medicine was poor and relapse rate was high which also have impact on the life of caregiver.
Profile of Cosmetic Consultation in a General Dermatology Clinic
Authors: Thapa Deeptara Pathak , Pradhan S
Published: December 2019

Cosmetic Dermatology has garnered tremendous interest all over the world. With the advent of modern technologies and developments, demand for non-surgical aesthetic procedures is on a rise. The purpose of the study is to know the types of cosmetic consultations and procedures being done in a General Dermatology setup. Patients who visited outpatient dermatology department of a multi-specialty, Nidan Hospital, Lalitpur, between May 2017 to April 2018 were included. Details of patients’ demographic profile were filled in a preset performa. Other information related to cosmetic consultation including dermatological diagnosis, awareness of cosmetic procedures and cosmetic procedures etc were also included. 160 patients attended the dermatology outpatient during the study period. Seventy four percent of them were females. Thirty six percent of the cases were between 26years and 35 years. Forty three percent of the cohort were working. Majority of them had Bachelors degree (57%). Twenty six patients who attended the OPD wanted cosmetic consultation. The type of cosmetic consultation, majority sought for was wrinkles (37%). Among the cosmetic procedures performed, chemical peeling was done in 59%, mesotherapy in 16%, mole removal in 12% and remaining 13% underwent topical treatment. Statistical significance was seen between Cosmetic consultation and economic status (p=0.000) and between cosmetic consultation and employment status (p=0.007). Almost a quarter of patients attending a general dermatology OPD sought cosmetic consultation. Females, young adults who are employed, well-educated and from good socio-economic status seek cosmetic services.