bluuebunny/crossref_metadata_2025 huggingface dataset:
Nationwide study of eculizumab in paroxysmal nocturnal hemoglobinuria: Evaluation of treatment indications and outcomes
Authors: Schaap Charlotte C. M. , Heubel‐Moenen Floor C. J. I. , Nur Erfan , Bartels Marije , van der Heijden Olivier W. H. , de Jonge Emiel , Preijers Frank W. M. B. , Blijlevens Nicole M. A. , Langemeijer Saskia M. C. ,   the Dutch PNH Working Group
Published: February 2023

Eculizumab is an effective treatment for paroxysmal nocturnal hemoglobinuria (PNH). However, considering the risk of life‐threatening meningococcal disease, life‐long duration and costs, there are strict criteria for initiation of therapy. To evaluate the application and real‐world effectiveness of eculizumab in the Netherlands, a multicenter retrospective cohort study was conducted: indications and treatment outcomes were collected for 105 Dutch PNH patients. In all patients, eculizumab was initiated conforming to indications as formulated in the Dutch PNH guideline. According to recently published response criteria, 23.4% of the patients had reached a complete hematological response, 53.2% a good or partial response, and 23.4% a minor response after 12 months of therapy. In the majority of patients the response remained stable during long‐term follow‐up. The degree and relevance of extravascular hemolysis significantly differed between response groups (p = 0.002). Improvements of EORTC‐QLQc30 and FACIT‐fatigue scores were observed, however patients reported lower scores than the general population. A detailed evaluation of 18 pregnancies during eculizumab showed no maternal or fetal deaths, and no thromboembolic events during pregnancy. This study demonstrates that the majority of patients benefit from eculizumab when adhering to the indications as formulated in the Dutch PNH guideline. However, novel therapies are needed to further improve real‐world outcomes, such as hematological responses and quality of life.
Retention of passive integrated transponder tags in hatchery brook trout: Effect of tag size, implantation site, and double tagging
Authors: Peterson Douglas P. , Twibell Ronald G. , Piteo Matthew S.
Published: February 2023

A fundamental assumption of mark‐recapture studies is that individuals do not lose their marks or tags, because tag loss can lead to biased parameter estimates. Retention of 8‐mm full‐duplex and 12‐mm half‐duplex passive integrated transponder (PIT) tags implanted in the abdomen, opercular musculature, and dorsal sinus of age‐0+ hatchery male brook trout (Salvelinus fontinalis) was evaluated in the laboratory. Treatments included single‐ and double‐tagged fish. Survival, growth, and tag retention were monitored for 181–187 days for 640 tagged fish and 80 untagged controls. Survival to 180 days ranged 95%–100% and was not affected by tagging. PIT‐tagging caused a short‐term reduction in growth rates. Tag retention to 180 days was 100% for dorsal implantation, 83.0% with 8‐mm tags in the operculum, 97.5% with 8‐mm tags in the abdomen, and 99.6% with 12‐mm tags in the abdomen. Retention of opercular tags was positively related to size at tagging. Tag loss was independent of whether fish were single‐ or double‐tagged. Double tagging with one full‐ and one half‐duplex PIT tag in different body locations would hedge against data loss relative to single tagging, especially for longer‐lived and iteroparous species where tag loss through time is more likely.
Steroid administration for ischemic complications after radiofrequency ablation: A retrospective study
Authors: Wake Taijiro , Tateishi Ryosuke , Moriyama Makoto , Fukumoto Tsuyoshi , Yamada Tomoharu , Nakagomi Ryo , Nakatsuka Takuma , Sato Masaya , Minami Tatsuya , Uchino Koji , Nakagawa Hayato , Shiina Shuichiro , Koike Kazuhiko , Fujishiro Mitsuhiro
Published: February 2023

Aim

The aim of this study was to evaluate the effects of steroids on ischemic complications after radiofrequency ablation.

Methods

A total of 58 patients with ischemic complications were divided into two groups according to corticosteroid use or non‐use.

Results

A total of 13 patients who were administered steroids had a shorter duration of fever than those who were not administered steroids (median 6.0 vs. 2.0 days; p < 0.001). Linear regression analysis showed that steroid administration was associated with a reduction of 3.9 days in the duration of fever (p = 0.008).

Conclusions

Steroid administration for ischemic complications after radiofrequency ablation may reduce the risk of fatal outcomes by blocking systemic inflammatory reactions.
Seasonal variability in body mass index change among children enrolled in the Pediatric Obesity Weight Evaluation Registry: A step in the right direction
Authors: Lane Taylor S. , Sonderegger Derek L. , Binns Helen J. , Kirk Shelley , Christison Amy L. , Novick Marsha , Tucker Jared , King Eileen , Wallace Stephenie , Brazendale Keith , Kharofa Roohi Y. , Walka Sherry , Heer Hendrik ‘Dirk’ De ,   the POWER Work Group
Published: February 2023

Background

Research has shown children disproportionately gain excess weight over the summer months (vs. school months), with stronger effects for children with obesity. However, the question has not been investigated among children receiving care in paediatric weight management (PWM) programs.

Objective

To test for seasonal variability in weight change among youth with obesity in PWM care enrolled in the Pediatric Obesity Weight Evaluation Registry (POWER).

Method

Longitudinal evaluation of a prospective cohort from 2014 to 2019 among youth in 31 PWM programs. Change in percentage of the 95th percentile for BMI (%BMIp95) was compared by quarter.

Results

Participants (N = 6816) were primarily ages 6–11 (48%), female (54%), 40% non‐Hispanic White, 26% Hispanic and 17% Black, and 73% had severe obesity. Children were enrolled on average 424.9 ± 401.5 days. Participants reduced their %BMIp95 every season, but compared with Quarter 3 (July–September), reductions were significantly greater in Q1 (Jan‐March, b = −0.27, 95%CI −0.46, −0.09), Q2 (April–June, b = −0.21, CI −0.40, −0.03), and Q4 (October–December, b = −0.44, CI −0.63, −0.26).

Conclusion and Relevance

Across 31 clinics nationwide, children reduced their %BMIp95 every season, but reductions were significantly smaller during the summer quarter. While PWM successfully mitigated excess weight gain during every period, summer remains a high‐priority time.
Patient admission scheduling problems with uncertain length of stay: optimization models and an efficient matheuristic approach
Authors: Guido Rosita
Published: February 2023

In this paper, we study two well‐known problems that are attracting increasing attention in the last years: patient admission and patient‐to‐room assignment problems. These two problems are often complicated by some patients, which may have fluctuations in length of stay (LOS). We propose an optimization model that plans patient admissions and patient stays considering fluctuations in LOS and does not allow overcrowded rooms, as typically required in real‐world cases. We develop an efficient matheuristic approach based on large neighborhood search and fix and optimize heuristic. We tested our optimization model and some variations of the objective function on benchmark instances from the literature. The computational results indicate the effectiveness of the proposed methods in improving the quality of care offered to the patients. Furthermore, the proposed approach can provide a useful support to decision makers for patient flow management and avoid disruptions in access to care due to bed shortages.
Can triage nurses accurately interpret the electrocardiogram in the emergency department to predict acute cardiovascular events? A prospective observational study
Authors: Sibilio Serena , Zaboli Arian , Magnarelli Gabriele , Canelles Massimiliano Fanni , Rella Eleonora , Pfeifer Norbert , Brigo Francesco , Turcato Gianni
Published: February 2023

Aims

The prompt recording of the electrocardiogram (ECG) and its correct interpretation is crucial to the management of patients who present to the emergency department (ED) with cardiovascular symptoms. Since triage nurses represent the first healthcare professionals evaluating the patient, improving their ability in interpreting the ECG could have a positive impact on clinical management. This real‐world study investigates whether triage nurses can accurately interpret the ECG in patients presenting with cardiovascular symptoms.

Design

Prospective, single‐centre observational study conducted in a general ED of General Hospital of Merano in Italy.

Methods

For all patients included, the triage nurses and the emergency physicians were asked to independently interpret and classify the ECGs answering to dichotomous questions. We correlated the interpretation of the ECG made by the triage nurses with the occurrence of acute cardiovascular events. The inter‐rater agreement in ECG interpretation between physicians and triage nurses was evaluated with Cohen's kappa analysis.

Results

Four hundred and ninety‐one patients were included. The inter‐rater agreement between triage nurses and physicians in classifying an ECG as abnormal was good. Patients who developed an acute cardiovascular event were 10.6% (52/491), and in 84.6% (44/52) of them, the nurse accurately classified the ECG as abnormal, with a sensitivity of 84.6% and a specificity of 43.5%.

Conclusion

Triage nurses have a moderate ability in identifying alterations in specific components of the ECG but a good ability in identifying patterns indicative of time‐dependent conditions correlated with major acute cardiovascular events.

Impact for Nursing

Triage nurses can accurately interpret the ECG in the ED to identify patients at high risk of acute cardiovascular events.

Reporting Method

The study was reported according to the STROBE guidelines.

No Patient or Public Contribution

The study did not involve any patients during its conduction.
Exploring the emergence of waste recovery and exchange in industrial clusters
Authors: Noori Shiva , Korevaar Gijsbert , Stikkelman Rob , Ramírez Andrea
Published: February 2023

Self‐organized industrial symbiosis (IS) starts with one actor's decision to invest in a waste recovery plant and the other actors' decision to buy the recovered flow. Technical and institutional conditions of the cluster influence actors' decisions. This paper explores the emergence of IS collaborations in industrial clusters under different techno‐economic conditions in the long term. We propose a mixed‐integer linear programming model that incorporates costs and constraints associated with waste recovery and exchange to study actors' investment decisions and investigate shaped symbiotic exchanges under rising energy prices and limited electricity supply. The approach is implemented in Iran's Persian Gulf Mining and Metals Special Economic Zone as a case study. The results revealed that changes in internal or external condition simultaneously influence the industrial and waste recovery plants. For instance, increasing energy prices without raising product prices significantly decreased the production level of industrial plants and, consequently, heat recovery potential. Furthermore, the waste heat recovery plants' contribution to improving the cluster's economic and environmental performance was not the same. Electricity recovery from a power plant's waste heat can result in 55 PJ grid electricity intake reduction and 720 M€ cluster cash flow increase. Recovered cooling or electricity from the steelmaking plant waste heat was consumed internally rather than shaping IS. These model outcomes show its capability to study IS within the socio‐technical structure of the cluster, not a standalone phenomenon. Implemented conceptualization offers a novel system‐level approach, which could be adjusted to assess other industrial development strategies.
Interventions to Reduce Inpatient and Discharge Opioid Prescribing for Postpartum Patients: A Systematic Review
Authors: Badreldin Nevert , Ditosto Julia D. , Holder Kai , Beestrum Molly , Yee Lynn M.
Published: February 2023

Introduction

As deaths related to opioids continue to rise, reducing opioid use for postpartum pain management is an important priority. Thus, we conducted a systematic review of postpartum interventions aimed at reducing opioid use following birth.

Methods

From database inception through September 1, 2021, we conducted a systematic search in Embase, MEDLINE, Cochrane Library, and Scopus including the following Medical Subject Heading (MeSH) terms: postpartum, pain management, opioid prescribing. Studies published in English, restricted to the United States, and evaluating interventions initiated following birth with outcomes including an assessment of change in opioid prescribing or use during the postpartum period (<8 weeks postpartum) were included. Authors independently screened abstracts and full articles for inclusion, extracted data, and assessed study quality using the Grading of Recommendations, Assessment, Development, and Evaluation tool and risk of bias using the Institutes of Health Quality Assessment Tools.

Results

A total of 24 studies met inclusion criteria. Sixteen studies evaluated interventions aimed at reducing postpartum opioid use during the inpatient hospitalization, and 10 studies evaluated interventions aimed at reducing opioid prescribing at postpartum discharge. Inpatient interventions included changes to standard order sets and protocols for the management of pain after cesarean birth. Such interventions resulted in significant decreases in inpatient postpartum opioid use in all but one study. Additional inpatient interventions, including use of lidocaine patches, postoperative abdominal binder, valdecoxib, and acupuncture were not found to be effective in reducing postpartum opioid use during inpatient hospitalization. Interventions targeting the postpartum period included individualized prescribing and state legislative changes limiting the duration of opioid prescribing for acute pain both resulted in decreased opioid prescribing or opioid use.

Discussion

A variety of interventions aimed at reducing opioid use following birth have shown efficacy. Although it is not known if any single intervention is most effective, these data suggest that implementation of any number of interventions may be advantageous in reducing postpartum opioid use.
Evaluation of the trueness and precision of conventional impressions versus digital scans for the all‐on‐four treatment in the maxillary arch: An in vitro study
Authors: Marshaha Nour Jamal , Azhari Amr Ahmed , Assery Mansour K , Ahmed Walaa Magdy
Published: February 2023

Purpose

To compare the accuracy of digitizing conventional impressions to intraoral surface scans for all‐on‐four treatment in the maxillary arch.

Materials and methods

An edentulous maxillary arch model with four implants placed in an all‐on‐four design was fabricated. Intraoral surface scans (n = 10) were obtained using an intraoral scanner after scan body insertion. For conventional polyvinylsiloxane impressions of the model, implant copings were inserted into the implant fixation for implant level, opened tray impressions (n = 10). The model and conventional impressions were digitized to obtain digital files. A reference file was created using a laboratory‐scanned conventional standard tessellation language (STL) file with analog to scan the body using exocad software. STL datasets from the two digital and conventional impression groups were superimposed with reference files to assess the 3D deviations. Two‐way ANOVA and paired‐samples t‐test was performed to assess the difference in trueness and examine the effects of impression technique and implant angulation on the deviation amount.

Results

No significant differences were found between the conventional impression and intraoral surface scan groups F(1, 76) = 2.705, p = 0.104. No significant differences were found between conventional straight and digital straight implants and between conventional and digital tilted implants F(1, 76) = .041, p = 0.841. No significant differences were found between conventional straight and conventional tilted implants p = 0.07 and between digital straight and digital tilted implants p = 0.08.

Conclusion

Digital scans were more accurate than conventional impressions. The digital straight implants were more accurate than the conventional straight implants, and the digital tilted implants were more accurate than the conventional tilted implants, with higher accuracy for digital straight implants.
Effects of School Policies and Programs on Violence Among All High School Students and Sexual and Gender Minority Students
Authors: Cruz Theresa H. , Ross‐Reed Danielle E. , FitzGerald Courtney A. , Overton Kathryn , Landrau‐Cribbs Erica , Schiff Melissa
Published: February 2023

BACKGROUND

Youth identifying as lesbian, gay, bisexual (LGB) and/or transgender/gender nonconforming (TGNC) are at increased risk of violence. School policies and practices may mitigate this risk.

METHODS

Researchers merged data from the 2016 New Mexico School Health Profiles and the 2017 New Mexico Youth Risk and Resiliency Survey. Researchers employed multivariable logistic regression to test the associations between school‐level measures and violence outcomes.

RESULTS

Genders and sexualities alliances (GSAs) were associated with reduced odds of lifetime forced sex among all, heterosexual cisgender, and LGB students, reduced odds of sexual violence among heterosexual cisgender students, and reduced odds of dating violence among LGB students. Inclusive sexual health education was associated with reduced odds of lifetime forced sex among LGB and TGNC students, reduced odds of sexual violence among LGB students, and increased odds of dating violence among heterosexual cisgender students. Inclusive teacher training was associated with increased odds of lifetime forced sex among TGNC students.

IMPLICATIONS FOR SCHOOL HEALTH POLICY, PRACTICE, AND EQUITY

Inclusive sexual health education and the presence of active GSAs may have the greatest potential for reducing violence, especially among LGB and TGNC students.

CONCLUSIONS

Findings highlight the important role of school policies and practices in addressing violence.
Natural occurrence of cucumber mosaic virus infecting Physalis peruviana plants in Brazil
Authors: Bello Vinicius Henrique , Carpim Larissa , Lorenzi Harri José , Rezende Jorge Alberto Marques , Kitajima Elliot Watanabe
Published: February 2023

In 2020, virus‐like symptoms such as leaf size reduction, mosaic, and blistering were observed in Physalis peruviana plants in Brazil. Isometric particles ca. 30 nm in diameter were observed by transmission electron microscope in leaf extracts of field‐symptomatic plants. Based on molecular assays and nucleotide sequencing of amplicons, cucumber mosaic virus (CMV, genus Cucumovirus) was identified in mixed infection with physalis rugose mosaic virus (PhyRMV, genus Sobemovirus). Phylogenetic analysis indicated that the CMV belongs to the subgroup IA. Symptoms like those of field symptomatic plants were observed on mechanically inoculated plants of P. peruviana. RT‐PCR confirmed CMV and PhyRMV infection. CMV was transmitted by Aphis gossypii to P. peruviana plants. This is the first report of CMV infecting P. peruviana plants in Brazil.
Affective Priming Effects by Task‐Relevant and Task‐Irrelevant Emotional Pictures: An ERP Study
Authors: Zhu Anqi , Takeda Yuji
Published: February 2023

The present study examined how task‐relevant and task‐irrelevant prime stimuli influence the affective priming effect. An emotional picture (unpleasant or neutral) and a checkerboard picture were presented simultaneously as a prime stimulus. Participants were required to pay attention to either the emotional picture (EP‐attended condition) or the checkerboard picture (CP‐attended condition). An emotionally neutral picture (target) was then presented, and participants were asked to evaluate the emotional valence of the target. The evaluation score was more negative following the unpleasant prime picture, and this priming effect was larger in the EP‐attended condition, in which the emotional prime picture was task‐relevant than in the CP‐attended condition, in which the emotional prime picture was task‐irrelevant. The late positive potentials (LPPs) elicited by the prime stimuli were larger when the emotional prime picture was unpleasant, whereas the LPPs elicited by the target stimuli were smaller when the emotional prime picture was unpleasant. More importantly, significant association between the LPP amplitudes and the magnitude of affective priming effects were observed in the EP‐attended condition but not in the CP‐attended condition. This indicates that the degree of the affective priming effect induced by the task‐relevant prime stimulus was related to attentional resource allocation, but this was not the case for the affective priming effect induced by the task‐irrelevant prime stimulus. The present results suggest that affective priming effects induced by task‐relevant and task‐irrelevant prime stimuli occur in a different manner in terms of attentional processing.
Declining Quantity and Quality of Births in Chile amidst the COVID‐19 Pandemic
Authors: Pesando Luca Maria , Abufhele Alejandra
Published: February 2023

Extensive demographic scholarship shows that the population‐level implications of mortality crises such as the COVID‐19 pandemic extend beyond mortality dynamics to affect fertility and family‐formation strategies. Using novel municipality‐level data from Chile covering all births that occurred between January 2017 and December 2021, this study explores trends in fertility and implications of the COVID‐19 pandemic for “quantum” and “quality” of births in the Chilean context. Building both a monthly and a yearly panel of 346 municipalities and leveraging fixed‐effects regression analyses, we focus on births and crude birth rates to measure quantum, while quality is assessed through the share of births that are low‐weight (LBW) and preterm (PTB). Our findings provide evidence of a significant drop in fertility in the wake of COVID—of the magnitude of a reduction of 1.3 live births per 1,000 individuals—which reaches a minimum around February 2021, followed by an incipient rebound in late 2021. Moreover, estimates on child health at birth suggest that the COVID period was associated with an increase in LBW and, foremost, PTB, by 1 and 2.2 percentage points, respectively. Findings from this study shed light on the role of policy interventions in the health arena and the linkages between short‐ and long‐run effects in relation to the various COVID‐19 waves in Chile.
Classifying asthma control using salivary and fecal bacterial microbiome in children with moderate‐to‐severe asthma
Authors: Blankestijn Jelle M. , Lopez‐Rincon Alejandro , Neerincx Anne H. , Vijverberg Susanne J. H. , Hashimoto Simone , Gorenjak Mario , Sardón Prado Olaia , Corcuera‐Elosegui Paula , Korta‐Murua Javier , Pino‐Yanes Maria , Potočnik Uroš , Bang Corinna , Franke Andre , Wolff Christine , Brandstetter Susanne , Toncheva Antoaneta A. , Kheiroddin Parastoo , Harner Susanne , Kabesch Michael , Kraneveld Aletta D. , Abdel‐Aziz Mahmoud I. , Maitland‐van der Zee Anke H. ,   the SysPharmPediA Consortium
Published: February 2023

Background

Uncontrolled asthma can lead to severe exacerbations and reduced quality of life. Research has shown that the microbiome may be linked with asthma characteristics; however, its association with asthma control has not been explored. We aimed to investigate whether the gastrointestinal microbiome can be used to discriminate between uncontrolled and controlled asthma in children.

Methods

143 and 103 feces samples were obtained from 143 children with moderate‐to‐severe asthma aged 6 to 17 years from the SysPharmPediA study. Patients were classified as controlled or uncontrolled asthmatics, and their microbiome at species level was compared using global (alpha/beta) diversity, conventional differential abundance analysis (DAA, analysis of compositions of microbiomes with bias correction), and machine learning [Recursive Ensemble Feature Selection (REFS)].

Results

Global diversity and DAA did not find significant differences between controlled and uncontrolled pediatric asthmatics. REFS detected a set of taxa, including Haemophilus and Veillonella, differentiating uncontrolled and controlled asthma with an average classification accuracy of 81% (saliva) and 86% (feces). These taxa showed enrichment in taxa previously associated with inflammatory diseases for both sampling compartments, and with COPD for the saliva samples.

Conclusion

Controlled and uncontrolled children with asthma can be differentiated based on their gastrointestinal microbiome using machine learning, specifically REFS. Our results show an association between asthma control and the gastrointestinal microbiome. This suggests that the gastrointestinal microbiome may be a potential biomarker for treatment responsiveness and thereby help to improve asthma control in children.
Mother and clinician experiences of a trial of a video feedback parent–infant intervention for mothers experiencing difficulties consistent with ‘personality disorder’: A qualitative interview study
Authors: Barnicot Kirsten , Parker Jennie , Kalwarowsky Sarah , Stevens Eloise , Iles Jane , Ramchandani Paul , Crawford Mike
Published: February 2023

Objectives

We explored mothers' and clinicians' experiences of a video feedback intervention adapted for perinatal ‘personality disorder’ (VIPP‐PMH) and the acceptability of a randomised controlled trial (RCT) examining its effectiveness.

Design

In‐depth qualitative interviews with participants from a two‐phase feasibility study of the VIPP‐PMH intervention. Participants were mothers experiencing enduring difficulties in managing emotions and relationships, consistent with a ‘personality disorder’, and their 6‐ to 36‐month‐old children.

Methods

Forty‐four qualitative interviews were conducted, including all nine mothers receiving VIPP‐PMH during the pilot phase, 25 of the 34 mothers participating in the RCT (14 allocated to the VIPP‐PMH arm and 9 from the control arm), 11 of the 12 clinicians delivering VIPP‐PMH and one researcher. Interview data were thematically analysed.

Results

Mothers described feeling motivated to take part in the research and understood the need for randomisation. Research visits were largely experienced positively, with some suggestions for improvement in questionnaire timing and accessibility. Almost all mothers initially felt anxious about being filmed, but reported positive experiences of the intervention, particularly valuing its non‐judgemental, positive and child‐focussed nature, their supportive relationship with the therapist and the insights they gained on their child.

Conclusions

The findings indicate the likely feasibility and acceptability of undertaking a future definitive RCT of the VIPP‐PMH intervention in this population. In designing a future trial, a positive and non‐judgemental therapeutic relationship will be important to allay mothers' anxieties about being filmed, and careful consideration should be given to the timing and accessibility of questionnaires used.
Killer to cure: Expression and production costs calculation of tobacco plant‐made cancer‐immune checkpoint inhibitors
Authors: Ridgley Laura A. , Falci Finardi Nicole , Gengenbach Benjamin B. , Opdensteinen Patrick , Croxford Zack , Ma Julian K.‐C. , Bodman‐Smith Mark , Buyel Johannes F. , Teh Audrey Y.‐H.
Published: February 2023

Immune checkpoint inhibitors (ICIs) have achieved huge clinical success. However, many still have limited response rates, and are prohibitively costly. There is a need for effective and affordable ICIs, as well as local manufacturing capacity to improve accessibility, especially to low‐to‐middle income countries (LMICs). Here, we have successfully expressed three key ICIs (anti‐PD‐1 Nivolumab, anti‐NKG2A Monalizumab, and anti‐LAG‐3 Relatimab) transiently in Nicotiana benthamiana and Nicotiana tabacum plants. The ICIs were expressed with a combination of different Fc regions and glycosylation profiles. They were characterized in terms of protein accumulation levels, target cell binding, binding to human neonatal Fc receptors (hFcRn), human complement component C1q (hC1q) and various Fcγ receptors, as well as protein recovery during purification at 100 mg‐ and kg‐scale. It was found that all ICIs bound to the expected target cells. Furthermore, the recovery during purification, as well as Fcγ receptor binding, can be altered depending on the Fc region used and the glycosylation profiles. This opens the possibility of using these two parameters to fine‐tune the ICIs for desired effector functions. A scenario‐based production cost model was also generated based on two production scenarios in hypothetical high‐ and low‐income countries. We have shown that the product accumulation and recovery of plant production platforms were as competitive as mammalian cell‐based platforms. This highlights the potential of plants to deliver ICIs that are more affordable and accessible to a widespread market, including LMICs.
Cross‐temporal grounding
Authors: Correia Fabrice , Merlo Giovanni
Published: February 2023

Cross‐temporal grounding is a type of grounding whereby present facts about the past (for example that Caesar was alive) are explained in terms of past facts (for example that Caesar is alive) rather than in terms of other present facts. This paper lays the foundations for a theory of cross‐temporal grounding. After introducing the general idea of a type of grounding connecting facts to past facts, we offer two arguments that past‐directed facts require cross‐temporal grounds—the ‘argument from intimacy’ and the ‘argument from past explanation’. We then go on to show that cross‐temporal grounding statements can be understood as instances of a type of explanation which is perfectly legitimate and intelligible, even if somewhat unusual. Finally, we explain how the logic of grounding can be extended to accommodate cross‐temporal grounding statements.
Full genomic sequence of the HLA‐A*02:01:01:241 allele identified by next‐generation sequencing
Authors: Rahaman Motiur , Bhattacharya Shatarupa , Mukherjee Gayatri , Mukherjee Budhaditya , Chakravorty Nishant
Published: February 2023

HLA‐A*02:01:01:241 differs from HLA‐A*02:01:01:01 by a single nucleotide substitution at position 178 in intron 1.
Social Media and Advertising Literacy of the Older Adults
Authors: Čábyová Ľudmila , Hrušková Alena , Rybnikárová Martina
Published: February 2023

In their study on advertising literacy, the authors focus their attention on the target group of  the older adults This is a very attractive target group that is always eager to shop and try new products. They also have sufficient financial resources to meet their interests and needs. This target group is also very attractive to advertisers, but their ability to understand advertising may be impaired compared to younger target groups. This is due to the use of new digital forms of advertising, which may not be as comprehensible to older adults. In their study, the authors surveyed the level of advertising literacy in a sample of older adults. The aim was to determine whether older adults understood the intent and objectives of advertising and whether they were able to identify the advertiser (advertiser). The authors also investigated the advertising literacy of older adults in relation to other marketing communication tools, such as public relations, product placement, and social media advertising.
Reviewer Acknowledgements for Studies in Media and Communication, Vol. 11, No. 1
Authors: Johnson Patricia
Published: February 2023

Studies in Media and Communication (SMC) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether SMC publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 11, Number 1Aina Fernàndez Aragonès, Tecnocampus-UPF, SpainAmie Jones, University of Georgia, USAAnita Šulentić, Edward Bernays University College, CroatiaAyşe Aslı Sezgin, Çukurova University, TurkeyCamelia Cmeciu, Danubius University of Galati, RomaniaGiorgio Poletti, University of Ferrara, ItalyHerman, Universitas HKBP Nommensen Pematangsiantar, IndonesiaImed Ben Labidi, Doha Institute for Graduate Studies, USAKrešimir Dabo, Edward Bernays University College, CroatiaKrešimir Žažar, University of Zagreb, CroatiaMatthias Degen, Westphalian University of Applied Sciences, GermanyMiguel Álvarez-Peralta, University Rey Juan Carlos, SpainMunira Fayzulloeva, Tajik State University of Law, TajikistanNavid Darvishzadeh, Georgia State University, USANingyang Chen, Soochow University, ChinaPadilla-Castillo Graciela, Complutense University of Madrid, SpainRafael Angel Salazar Martínez, University of Holguin-Mayari Municipality Center, CubaRebeca Suarez-Alvarez, Rey Juan Carlos University, SpainSixto-García José, University of Santiago de Compostela, SpainVerlumun Celestine Gever, University of Nigeria, NigeriaXiao Li, Hubei Institute of Fine Arts, ChinaXin Wang, North China Institute of Science and Technology, ChinaYoung Joon Lim, University of Texas Rio Grande Valley, USAYufan Sunny Qin, James Madison University, USA Patricia JohnsonEditorial AssistantOn behalf of,The Editorial Board of Studies in Media and CommunicationRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAURL: http://smc.redfame.com
Modelling and validation of defects on infrasound wind-noise-reduction pipe systems
Authors: Kristoffersen Samuel K. , Vincent Paul , Le Pichon Alexis , Denis Stéphane , Larsonnier Franck , Alcoverro Benoit , Gabrielson Thomas B.
Published: February 2023

Infrasound signals are detectable from many different sources, such as earthquakes and man-made explosions. Wind-generated turbulent noise can mask incoming infrasound signals; however, pipe-array wind-noise-reduction systems (WNRSs) have been designed to reduce the level of noise in the observed pressure time series. Given that the arrival times of the signals need to be well-known to calculate the source back azimuth and trace velocity, the response of the WNRS must be known in magnitude and phase. Previous work has been performed to optimize these systems and effectively model them. The goal of this research is to determine the effects of different defects which may occur during normal operation in typical field-experiment conditions. The models were extended to include the effects of defective systems, such as blockages or leaks. It was found that these models could effectively recreate the responses observed in an experimental setting, and several different defects were tested and are summarized in this paper.
Critiquing the Social Media Scholarship in Sport Studies: A Sport Entrepreneurship Analysis
Authors: Ratten Vanessa
Published: February 2023

Social media is endemic to the sport industry and ensures its global competitiveness. The aim of this article is to critique social media scholarship in sport studies by taking a sport entrepreneurship perspective. This approach is useful in expanding and building on the existing social media research by focusing on the innovation apparent in the sport industry. An overview of current literature on social media and sport entrepreneurship is discussed. This article concludes with a number of different research trajectories that are needed to advance the practice and scholarship of sport social media studies and sport entrepreneurship.
Hyperprofessionalized and Commodified: A Case Study Examination of FBS Bowl Games and the Utilization of Football Players as Programmatic Promotional Material
Authors: Corr Chris , Southall Richard M. , Southall Crystal , Hart Richard J.
Published: February 2023

Football Bowl Subdivision (FBS) football games are presented in a hypercommercialized manner by television broadcast networks through the targeted use of in-game graphics and corporatized content. While commercialized FBS football broadcast components have been analyzed within the frameworks of a hypercommercialized National Collegiate Athletic Association and media institutional logics, an analysis of commentator language has yet to be examined within the larger institutional field of FBS football broadcasts. Utilizing agenda setting and media framing as frameworks, this case study examined the manner in which commentators frame FBS football players as professionals in a hypercommercialized institutional setting. From a sample of 18 FBS bowl games during the 2019–20 season, discourse and thematic analysis reveal that commentators frame FBS football players in the context of their future professional opportunities (i.e., National Football League). The framing of FBS football players as professionals aligns with extant literature examining the broader institutional field of broadcast media and logics pervasive in the National Collegiate Athletic Association as an organization. The commodification of FBS football players as integral components to strategic programmatic content promoting future broadcast programming is discussed.
Sport for Development and Decolonization in a Settler Colonial State: Physical Culture in the Lives of Indigenous Peoples Incarcerated in Canadian Prisons
Authors: Norman Mark , Clifford Alicia G. , Henry Robert
Published: February 2023

This article considers if sport, broadly defined, can be constructed as a decolonizing practice for Indigenous Peoples incarcerated in Canadian prisons. Situating our analysis within transformative and decolonizing approaches to sport for development, we bring together disparate literatures—on settler colonialism and Indigenous incarceration, decolonization and Indigenous resurgence, and sport and incarceration—to critically analyze possibilities and limitations of sport as a vehicle for decolonization in an inherently colonial institution. Recognizing the structural constraints to such a process, we also critique the settler colonial state and criminal justice system in which many Indigenous Peoples are enmeshed. The article contributes to sport for development’s ongoing engagement with issues of decolonization and criminal justice.
Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility
Authors: Islam Salim T. , Jolivet Nicolas Y. , Cuzin Clémence , Belgrave Akeisha M. , My Laetitia , Fleuchot Betty , Faure Laura M. , Mahanta Utkarsha , Kezzo Ahmad A. , Saïdi Fares , Sharma Gaurav , Fiche Jean-Bernard , Bratton Benjamin P. , Herrou Julien , Nollmann Marcelo , Shaevitz Joshua W. , Durand Eric , Mignot Tâm
Published: February 2023

The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focal-adhesion (bFA) sites to power gliding motility. Using total internal reflection fluorescence and force microscopies, we identify the von Willebrand A domain-containing outer-membrane (OM) lipoprotein CglB as an essential substratum-coupling adhesin of the gliding transducer (Glt) machinery at bFAs. Biochemical and genetic analyses reveal that CglB localizes to the cell surface independently of the Glt apparatus; once there, it is recruited by the OM module of the gliding machinery, a heteroligomeric complex containing the integral OM β barrels GltA, GltB, and GltH, as well as the OM protein GltC and OM lipoprotein GltK. This Glt OM platform mediates the cell-surface accessibility and retention of CglB by the Glt apparatus. Together, these data suggest that the gliding complex promotes regulated surface exposure of CglB at bFAs, thus explaining the manner by which contractile forces exerted by inner-membrane motors are transduced across the cell envelope to the substratum.
Giant proteins in a giant cell: Molecular basis of ultrafast Ca
2+
-dependent cell contraction
Authors: Zhang Jing , Qin Weiwei , Hu Che , Gu Siyu , Chai Xiaocui , Yang Mingkun , Zhou Fang , Wang Xueyan , Chen Kai , Yan Guanxiong , Wang Guangying , Jiang Chuanqi , Warren Alan , Xiong Jie , Miao Wei
Published: February 2023

The giant single-celled eukaryote,
Spirostomum
, exhibits one of the fastest movements in the biological world. This ultrafast contraction is dependent on Ca
2+
rather than ATP and therefore differs to the actin-myosin system in muscle. We obtained the high-quality genome of
Spirostomum minus
from which we identified the key molecular components of its contractile apparatus, including two major Ca
2+
binding proteins (Spasmin 1 and 2) and two giant proteins (GSBP1 and GSBP2), which act as the backbone and allow for the binding of hundreds of spasmins. The evidence suggests that the GSBP-spasmin protein complex is the functional unit of the mesh-like contractile fibrillar system, which, coupled with various other subcellular structures, provides the mechanism for repetitive ultrafast cell contraction and extension. These findings improve our understanding of the Ca
2+
-dependent ultrafast movement and provide a blueprint for future biomimicry, design, and construction of this kind of micromachine.
Biomolecular actuators for genetically selective acoustic manipulation of cells
Authors: Wu Di , Baresch Diego , Cook Colin , Ma Zhichao , Duan Mengtong , Malounda Dina , Maresca David , Abundo Maria P. , Lee Justin , Shivaei Shirin , Mittelstein David R. , Qiu Tian , Fischer Peer , Shapiro Mikhail G.
Published: February 2023

The ability to physically manipulate specific cells is critical for the fields of biomedicine, synthetic biology, and living materials. Ultrasound has the ability to manipulate cells with high spatiotemporal precision via acoustic radiation force (ARF). However, because most cells have similar acoustic properties, this capability is disconnected from cellular genetic programs. Here, we show that gas vesicles (GVs)—a unique class of gas-filled protein nanostructures—can serve as genetically encodable actuators for selective acoustic manipulation. Because of their lower density and higher compressibility relative to water, GVs experience strong ARF with opposite polarity to most other materials. When expressed inside cells, GVs invert the cells’ acoustic contrast and amplify the magnitude of their ARF, allowing the cells to be selectively manipulated with sound waves based on their genotype. GVs provide a direct link between gene expression and acoustomechanical actuation, opening a paradigm for selective cellular control in a broad range of contexts.
Reduced insulin signaling in neurons induces sex-specific health benefits
Authors: Baghdadi Maarouf , Nespital Tobias , Mesaros Andrea , Buschbaum Sandra , Withers Dominic J. , Grönke Sebastian , Partridge Linda
Published: February 2023

Reduced activity of insulin/insulin-like growth factor signaling (IIS) extends health and life span in mammals. Loss of the
insulin receptor substrate 1 (Irs1)
gene increases survival in mice and causes tissue-specific changes in gene expression. However, the tissues underlying IIS-mediated longevity are currently unknown. Here, we measured survival and health span in mice lacking IRS1 specifically in liver, muscle, fat, and brain. Tissue-specific loss of IRS1 did not increase survival, suggesting that lack of IRS1 in more than one tissue is required for life-span extension. Loss of IRS1 in liver, muscle, and fat did not improve health. In contrast, loss of neuronal IRS1 increased energy expenditure, locomotion, and insulin sensitivity, specifically in old males. Neuronal loss of IRS1 also caused male-specific mitochondrial dysfunction, activation of
Atf4
, and metabolic adaptations consistent with an activated integrated stress response at old age. Thus, we identified a male-specific brain signature of aging in response to reduced IIS associated with improved health at old age.
Acoustic streaming enabled moderate swimming exercise reduces neurodegeneration in
C. elegans
Authors: Bhadra Joyita , Sridhar Nakul , Fajrial Apresio Kefin , Hammond Nia , Xue Ding , Ding Xiaoyun
Published: February 2023

Regular physical exercise has been shown to delay and alleviate neurodegenerative diseases. Yet, optimum physical exercise conditions that provide neuronal protection and exercise-related factors remain poorly understood. Here, we create an Acoustic Gym on a chip through the surface acoustic wave (SAW) microfluidic technology to precisely control the duration and intensity of swimming exercise of model organisms. We find that precisely dosed swimming exercise enabled by acoustic streaming decreases neuronal loss in two different neurodegenerative disease models of
Caenorhabditis elegans
, a Parkinson’s disease model and a tauopathy model. These findings highlight the importance of optimum exercise conditions for effective neuronal protection, a key characteristic of healthy aging in the elderly population. This SAW device also paves avenues for screening for compounds that can enhance or replace the beneficial effects of exercise and for identifying drug targets for treating neurodegenerative diseases.
Making It Matter: Increasing Student-Perceived Value of Microbiology through Reflective and Critical News Story Analysis
Authors: Rholl Drew A. , Cheeptham Naowarat , Lal Archana , Kleinschmit Adam J. , Parks Samantha T. , Mestrovic Tomislav
Published: February 2023

Even before coverage and updates on COVID-19 became a daily event in mainstream news, mass media was already full of science-focused current events stories. While relevant to our everyday lives, many popular press science articles overstate conclusions, misstate details or, at worst, purposefully spread disinformation.
Glycogen Metabolism in Candida albicans Impacts Fitness and Virulence during Vulvovaginal and Invasive Candidiasis
Authors: Miao Jian , Regan Jessica , Cai Chun , Palmer Glen E. , Williams David L. , Kruppa Michael D. , Peters Brian M.
Published: February 2023

Glycogen is a highly branched polymer of glucose and is used across the tree of life as an efficient and compact form of energy storage. Whereas glycogen metabolism pathways have been studied in model yeasts, they have not been extensively explored in pathogenic fungi.
Chromosome-Level Genome Assembly of the Yeast Candida verbasci
Authors: Brejová Broňa , Hodorová Viktória , Lichancová Hana , Peričková Eunika , Šoucová Veronika Anna , Sipiczki Matthias , Vinař Tomáš , Nosek Jozef
Published: February 2023

Candida verbasci
is an anamorphic ascomycetous yeast. We report the genome sequence of its type strain, 11-1055 (CBS 12699). The nuclear genome assembly consists of seven chromosome-sized contigs with a total size of 12.1 Mbp and has a relatively low G+C content (28.1%).
Complete Genome Sequences of Bioluminescent Staphylococcus aureus Strains Xen31 and Xen36, Derived from Two Clinical Isolates
Authors: Blaise Océane , Leseigneur Clarisse , Cokelaer Thomas , Capuzzo Elena , Frescaline Nadira , Dussurget Olivier
Published: February 2023

Here, we report complete genome sequences of two clinical isolates of
Staphylococcus aureus
, namely, Xen31 and Xen36, which have been genetically modified to express an optimized
Photorhabdus luminescens
luciferase operon. Xen31 and Xen36 are bioluminescent strains used widely for investigation of bacterial pathogenesis, drug discovery, and development of novel therapies.
Complete Genome Sequence of Acinetobacter baumannii Strain KBN10P04593, a Clinical Isolate from South Korea
Authors: Islam Md. Maidul , Kim Jeongah , Kim Kyeongmin , Lee Je Chul , Shin Minsang
Published: February 2023

Acinetobacter baumannii
is an opportunistic nosocomial pathogen that is responsible for various life-threating infections in immunocompromised hosts. We present the complete 3.93-Mb genome sequence of
A. baumannii
KBN10P04593, generated by combining PacBio and Illumina technologies.