bluuebunny/crossref_metadata_2025 huggingface dataset:
Mobile-based in-home telerehabilitation compared with in-hospital face-to-face rehabilitation for elderly patients after total hip arthroplasty in China's level 1 trauma center: a noninferiority randomized controlled trial
Authors: Zhou Yang , Lyu Yiming , Wang Qiaojie , Ma Yanhong , Huang Lihua , Zhang Xin
Published: January 2025

Background

Telerehabilitation is gaining popularity in European and American countries, but whether it can be successfully implemented in China still lacks support from clinical studies.

Objective

This trial aimed to determine if a home-based telerehabilitation method is clinically noninferior to standard in-hospital face-to-face rehabilitation for elderly patients with total hip arthroplasty (THA) in China.

Methods

This multicenter randomized controlled trial was conducted from January 2021 to June 2022 at The First Rehabilitation Hospital in Shanghai, Shanghai Jiao Tong University affiliated Sixth People's Hospital and Shanghai Tongji University affiliated Tenth People's Hospital. Sixty-four patients were recruited for this two-arm, single-assessor blinded, randomized controlled trial. The participants were randomly assigned to the in-home telerehabilitation group (TELE group) and the in-hospital physical therapist in-person group (PT group). The intervention consisted of a 12-week home-based rehabilitation program with video instructions and remote coaching on a mobile APP (TELE group). The PT group received a standard in-hospital rehabilitation intervention assisted by a physical therapist for one month and outpatient clinic for the next two months. Patients were evaluated at baseline, 4 weeks, and 12 weeks postoperatively employing functional tests (Timed Up & Go test and Berg balance test) and self-reported questionnaires (Hip disability and Osteoarthritis Outcome Score (HOOS) and Short Form 12 (SF-12)).

Results

There was no significant difference between the two groups for the demographic and clinical characteristics. 61 participants were analyzed (PT group: n = 31, women: 48.4% of participants; TELE group: n = 30, 33.3% of participants) whose median age was 70 and 69 years, in PT group (IQR: 63–73) and TELE group (IQR: 66–72) respectively. At 12 weeks follow-up evaluation, the main differences between the two groups regarding the HOOS gains, adjusted for baseline values, were close to zero (P > 0.05). There was no significant difference in primary and secondary outcome measures between the two groups.

Conclusion

Our results showed the noninferiority of in-home telerehabilitation and advocated its application as a reliable alternative to in-hospital face-to-face rehabilitation for patients who underwent THA.

Clinical Trial Registration

https://www.chictr.org.cn/, Chinese Clinical Trial Registry (Number: ChiCTR1900025825).
Interactions Between Trace Elements and Macro Minerals in Pregnant Heifers
Authors: Dias Betini Raquel S. , López Secundino , Montanholi Yuri R. , France James
Published: January 2025

There is a lack of information on mineral interactions that take place during a heifer’s pregnancy when nutrient demand is high. The objective of the present study was to evaluate the interactions between the macro minerals calcium (Ca), phosphorus (P), magnesium (Mg), sulphur (S), sodium (Na) and potassium (K) and the trace elements copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), selenium (Se), zinc (Zn) and cobalt (Co) in pregnant heifers. Twenty-four pregnant heifers (age 18.2 ± 0.14 months; 87.5 ± 4.74 days in gestation; 497 ± 8.5 kg of body weight) were used in a 7 d digestibility trial (indirect method using lignin as a marker) during which they were fed a diet that provided minerals in accordance with NASEM requirements for gestating beef cattle. At the end of the digestibility trial, blood (jugular venipuncture) and liver (biopsy) samples were collected from each heifer. Multiple linear regression models were developed based on the main correlations and by considering collinearity effects between variables. Iron intake positively affected Mo concentration in serum, which was >10 µg/dL for most of the animals. Apparent absorption of Fe was negatively affected by Mo intake and Ca to P ratio in feed, whereas Se absorption was negatively affected by Fe intake and positively by Mo intake. The absorption coefficients of Mo and Fe were inversely related, emphasizing the interactions between these elements. Serum Fe and Cu in the liver were positively correlated, while liver Fe was inversely related to liver Mn. Fewer interactions were observed between the macro minerals. In conclusion, within the interactions observed, those between Fe and Mo deserve special attention, as high dietary Fe is commonly found in typical ruminant diets, and high serum Mo may indicate molybdenosis. Furthermore, high Fe intake appears to impact Se and Mn utilization.
Availability of Zinc, Copper, Iron, Manganese, and Selenium in Feed Ingredients and Sources in Pigs
Authors: Choi Yohan , Hong Junseon , Lee Jihwan , Kim Minju
Published: January 2025

This study evaluated the trace mineral availability of zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), and selenium (Se) in major feed ingredients, including corn, wheat, soybean meal (SBM), and fish meal (FM). Additionally, we assessed the bioavailability of these minerals in pigs supplemented with inorganic, organic, and nano-sized forms prepared via hot-melt extrusion (HME). A total of 64 barrows (Yorkshire × Landrace × Duroc crossbreds) with an average initial body weight of 26.61 ± 4.12 kg were housed individually in metabolic cages. Pigs were allocated to eight experimental diets in a completely randomized design, with eight replicates per diet group. The apparent total tract digestibility (ATTD) of Zn and Cu was significantly higher in SBM and FM than in the other ingredients (p < 0.05). SBM exhibited higher ATTD and standardized total tract digestibility (STTD) for Fe and Mn than corn, wheat, and FM (p < 0.05). Corn and wheat demonstrated significantly greater digestibility of Se than SBM and FM (p < 0.05). Supplementation with nano-sized minerals prepared by HME increased the digestibility of Zn and Cu, as well as their concentrations in pigs’ serum and liver, while reducing the fecal excretion of these minerals (p < 0.05). Organic mineral forms significantly enhanced Se bioavailability, improving its digestibility and concentrations in the liver and pancreas compared to the inorganic form (p < 0.05). In growth performance, organic and nano-sized mineral sources significantly improved growth rate without the increase in feed intake (p < 0.05). In conclusion, Zn and Cu from high-protein ingredients such as SBM and FM were more digestible than those from grains. Furthermore, plant-based ingredients, such as corn, wheat, and SBM, exhibited greater digestibility of Fe and Se. Among the trace mineral sources, the organic and HME-nano forms improved the bioavailability of Zn, Cu, and Se, while reducing their fecal excretion, thereby enhancing the efficiency of mineral utilization.
Optimization of Seed-Receiving Mechanism in Belt-Driven Seed Guide Tube Based on High-Speed Videography Experiment
Authors: Ma Chengcheng , Zhao Zhihuan , Chen Xiaomei , Tang Youyuan , Song Ning , Xiao Yanfeng , Yang Xu
Published: January 2025

During high-speed corn sowing at 10 km/h, the rapid seed discharge resulting from the high rotation speed of the seed disc escalates the impact force of seeds as they are released from the seed metering device into the seed guiding apparatus, consequently diminishing the overall seeding efficiency of the seeder. This study employed high-speed videography to conduct experiments and optimize parameters for the seed-receiving mechanism of a belt-driven seed guide tube. By changing the clamping wheel speed and seed-receiving angle, the speed change curve and displacement trajectory of seeds under different conditions were obtained and analyzed. The findings demonstrate that the seed speed fluctuation is more stable, and the seed displacement trajectory achieves greater stability at a clamping wheel speed of 560 r·min−1. When the seed-receiving angle is set at 85°, the seed speed fluctuation becomes less apparent, resulting in a smoother seed displacement trajectory. Finally, the experimental results of high-speed cameras are confirmed by field tests. The findings of this study can act as a theoretical basis for the further optimization of the experimental belt-driven seed guide tube.
Conspicuous Animals Remain Alert When Under Cover but Do Not Differ in the Temporal Course of Vigilance from Less Conspicuous Species
Authors: Hofmann Gerhard , Mettke-Hofmann Claudia
Published: January 2025

Conspicuousness increases the risk of predation. One strategy to reduce this risk is to increase vigilance. We investigated the frequency of head movements as a measure of vigilance at waterholes in two related songbird species that differed in their conspicuousness: the Gouldian finch and the long-tailed finch. While both species showed a high frequency of head movements (high vigilance) when sitting in an open tree, the more colourful Gouldian finch remained more vigilant than the less conspicuous long-tailed finch when sitting under cover. Moreover, juvenile Gouldian finches had similar vigilance levels than adults; whereas, juveniles of the long-tailed finch were less vigilant, possibly because of staying in the family group. Furthermore, both species showed a similar temporal course of vigilance with a fast decrease in vigilance after landing in the tree, which was affected by group size. Single individuals had much higher vigilance than groups of birds. Finally, vigilance was higher at small waterholes. The results show that conspicuousness, uncertainty when arriving at waterholes, and higher perceived risk at small waterholes increased vigilance.
High Antimicrobial Susceptibility of Cloacal Enterococci and Escherichia coli from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing Escherichia coli ST69
Authors: Cardona-Cabrera Teresa , Martínez-Álvarez Sandra , González-Azcona Carmen , Gijón-García Carlos Javier , Alexandrou Olga , Catsadorakis Giorgos , Azmanis Panagiotis , Torres Carmen , Höfle Ursula
Published: January 2025

Background/Objectives: In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This study determines the antimicrobial resistance rates of cloacal enterococci and Escherichia coli in DPs. Methods: Fifty-two blood and cloacal swab samples were collected from 31 nestlings (20 DP/11 great white pelicans) hatched after the H5N1 outbreak at the Prespa colony and 21 subadult/adult DPs captured at a spring migration stopover. The swabs were inoculated in non-selective and chromogenic-selective media. Identification was performed using MALDI-TOF, and antimicrobial susceptibility was tested. The genetic content was characterized using PCR and sequencing, and the clonality of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates was characterized using Multilocus Sequence Typing. Results: Twenty-eight non-repetitive E. coli and 45 enterococci isolates were recovered in non-selective media; most of them were susceptible to all antibiotics tested (85.7% E. coli/91.1% enterococci). Three of the fifty-two samples (6%, all adults) contained ESBL-E. coli isolates (detected in chromogenic ESBL plates), all carrying the blaCTX-M-15 gene and belonging to the lineage ST69. Conclusions: Despite the susceptibility of most fecal E. coli and enterococci isolates to all antibiotics tested, the finding that E. coli of lineage ST69 carry blaCTX-M-15 is of concern. This high-risk clone needs further investigation to elucidate its primary sources and address the growing threat of antimicrobial resistance from an integrated “One Health” perspective. Furthermore, it is imperative to study the potential impacts of ESBL-E. coli on the endangered DP further.
Predictive Model for Deformation of Adjacent Pipelines Caused by Tunnel Boring in Twin-Lane Tunnels in Soft Ground Layers
Authors: Wang Kezhong , Zheng Qianjiang , Hang Maowen
Published: January 2025

To create a discretized prediction model for the deformation of an adjacent pipeline, the pipeline structure is discretized, the differential equations governing the longitudinal deformation of the pipeline are inferred, and the displacement expressions and the solution methods of the virtual nodes of each unit are provided after discretization. This approach is based on the Pasternak foundation beam theory. It aims to address the issue of the difficulty in predicting the deformation of the adjacent pipeline caused by shield tunneling in a saturated soft ground layer in the Yangtze River Delta. The deformation pattern of the surrounding soil is determined and confirmed through additional numerical simulation, and the discretized prediction model is contrasted with the conventional Winkler foundation beam model and the Pasternak foundation beam model. The findings demonstrate that the discrete prediction model is simpler to solve and more accurately describes the deformation characteristics of the adjacent pipeline as well as the deformation distribution law. The calculated deformation characteristics primarily appear as the adjacent pipeline’s deformation due to the double tunnel boring exhibiting a “mono-peak shape” with a large middle and small ends, which is consistent with the actual situation. The two main factors influencing the pipeline deformation are the shield tunneling distance and pipeline spacing; the former has a negative correlation with the pipeline deformation, while the latter has a positive correlation. This work can offer a straightforward deformation prediction technique for shield tunneling in the Yangtze River Delta’s saturated soft ground next to existing pipelines.
Quaternary Segmentation Characteristics of the Hunhe Fault, Northeast China
Authors: Wan Bo , Ha Guanghao , Zhao Xiaohui , Suo Rui
Published: January 2025

The northern segment of the Tanlu fault zone, which encompasses the Dunhua–Mishan and Yilan–Yitong fault zones, plays a critical role in the tectonic framework of Northeast China. This study focuses on the Hunhe fault, part of the Liaoning segment of the Dunhua–Mishan fault zone, which exhibits concealed characteristics and an NE–NEE orientation. We employ remote sensing and field investigations to accurately delineate the Hunhe fault’s location, scale, and tectonic activity. The findings indicate that the Hunhe fault displays significant spatial variability in tectonic activity. Some segments show evidence of late Quaternary activity, contradicting prior research that classified the Hunhe fault as an active fault during the MIS (Marine Isotope Stages) 20-103MIS 20-103- MIS6-19MIS6-19 period and assessed its seismic potential differently. Recent field investigations suggest considerable spatial variability in tectonic activity, indicating segmental characteristics. In this study, the Hunhe fault is divided into segments based on five aspects: the fault structure and movement characteristics of the fault; transverse faults and obstruction structures; geological and geomorphological characteristics; seismic features; and fault activity. The detailed segments are as follows: the Shenyang segment, the Fushun segment, the Zhangdang-Nan Zamu segment, and the Nan Zamu to Ying Emeng East section. These findings aim to enhance the understanding of the seismic hazard potential associated with the Hunhe fault, highlighting the need for ongoing research to address its complexities and implications for regional seismic risk assessment.
Study on Hematological and Biochemical Index of Blood and Vitreous Humor in the Celestial-Eye Goldfish
Authors: Li Rongni , Sun Yansheng , Zhang Xin , Li Wentong
Published: January 2025

In this study, we conducted an exploration of blood collection, hematological and biochemical comparative analysis of the unique vitreous humor and blood of the celestial-eye goldfish (CE) (Carassius auratus), and obtained hematological and biochemical indicators for healthy celestial goldfish blood. After exploring the optimal method for collecting blood and ocular fluid, blood and vitreous humor samples were drawn from healthy CE, and an automatic hematology analyzer was used to measure 17 physiological indicators in the blood and vitreous humor. Additionally, 20 biochemical indices were measured by an automatic biochemical analyzer. Experiments were also conductedto evaluate the effect of blood and vitreous humor collection on the subsequent survival rate of the CE. The results showed that the blood of CE exhibited values for 17 hematological indicators, including red blood cells (mean value: 2.19 × 1012/L), white blood cells (mean value: 62.21 × 109/L), hemoglobin (mean value:138.25 g/L), and no eosinophils or basophils. We also found that the levels of aspartate amino transferase (AST) and glutamyl transpeptidase (GGT) in the vitreous humor of CE were significantly higher than those in the serum (p < 0.01). No cells were detected in the vitreous humor of CE, and there was no significant difference in survival rate compared to normal goldfish after extracting blood and vitreous humor from CE (p > 0.05). This study provides fundamental data for the cultivation of healthy goldfish, the development of goldfish hematology, and further research on the development and formation of CE’s unique eye traits.
Quantifying the Carbon Reduction Potential of Urban Parks Under Extreme Heat Events Using Interpretable Machine Learning: A Case Study of Jinan, China
Authors: Yu Lemin , Li Wenru , Zheng Changhui , Lin Xiaowen
Published: January 2025

Greenhouse gas emissions are primary drivers of climate change, and the intensification of extreme heat and urban heat island effects poses serious threats to urban ecosystems, public health, and energy consumption. This study systematically evaluated the carbon reduction potential of 369 urban parks in Jinan during extreme heat events using land surface temperature (LST) retrieval, combined with CatBoost + SHAP machine learning methods. Results indicate that the LST in Jinan ranged from 1.77 °C to 59.44 °C, and 278 parks exhibited significant cooling effects, collectively saving 2943 tons of CO2 per day—offsetting 11.28% of the city’s fossil fuel emissions. Small parks, such as community parks, demonstrated higher carbon-saving efficiency (CSE), while large ecological parks showed greater carbon-saving intensity (CSI). CSE was strongly correlated with vegetation coverage and surrounding population density, with efficiency increasing when the vegetation index was within 0.3–0.7 and population density ranged 0–5000 or 15,000–22,500 people. CSI was influenced by evapotranspiration and park geometric form, increasing significantly when the park area exceeded 250 hectares or evapotranspiration ranged 2.5–6.0. However, elevation and albedo negatively impacted both metrics, with the lowest CSI observed when elevation exceeded 150 m or albedo surpassed 18%.
Analyzing Gait Dynamics and Recovery Trajectory in Lower Extremity Fractures Using Linear Mixed Models and Gait Analysis Variables
Authors: Rezapour Mostafa , Seymour Rachel B. , Medda Suman , Sims Stephen H. , Karunakar Madhav A. , Habet Nahir , Gurcan Metin Nafi
Published: January 2025

In a prospective study, we examined the recovery trajectory of patients with lower extremity fractures to better understand the healing process in the absence of complications. Using a chest-mounted inertial measurement unit (IMU) device for gait analysis and collecting patient-reported outcome measures, we focused on 12 key gait variables, including Mean Leg Lift Acceleration, Stance Time, and Body Orientation. We employed a linear mixed model (LMM) to analyze these variables over time, incorporating both fixed and random effects to account for individual differences and the time since injury. This model also adjusted for varying intervals between assessments. Our study provided insights into gait recovery across different fracture types using data from 318 patients who experienced no complications or readmissions during their recovery. Through LMM analysis, we found that Tibia-Distal fractures demonstrated the fastest recovery, particularly in terms of mobility and strength. Tibia-Proximal fractures showed balanced improvements in both mobility and stability, suggesting that rehabilitation should target both strength and balance. Femur fractures exhibited varied recovery, with Diaphyseal fractures showing clear improvements in stability, while Distal fractures reflected gains in limb strength but with some variability in stability. To examine patients with readmissions, we conducted a Chi-squared test of independence to determine whether there was a relationship between fracture type and readmission rates, revealing a significant association (p < 0.001). Pelvis fractures had the highest readmission rates, while Tibia-Diaphyseal and Tibia-Distal fractures were more prone to infections, highlighting the need for enhanced infection control strategies. Femur fractures showed moderate readmission and infection rates, indicating a mixed risk profile. In conclusion, our findings emphasize the importance of fracture-specific rehabilitation strategies, focusing on infection prevention and individualized treatment plans to optimize recovery outcomes.
Molybdenum Can Regulate the Expression of Molybdase Genes, Affect Molybdase Activity and Metabolites, and Promote the Cell Wall Bio-Synthesis of Tobacco Leaves
Authors: Zhao Yuan , Zhang Yu , Zhang Kai , Tian Jiashu , Teng Huanyu , Xu Zicheng , Xu Jiayang , Shao Huifang , Jia Wei
Published: January 2025

Molybdenum (Mo) is widely used as a micronutrient fertilizer to improve plant growth and soil quality. However, the interactions between cell wall biosynthesis and molybdenum have not been explored sufficiently. This study thoroughly investigated the regulatory effects of different concentrations of Mo on tobacco cell wall biosynthesis from physiological and metabolomic aspects. The results indicate that Mo treatment increased the Mo content of tobacco variety K326. Moreover, it significantly up-regulated the gene expression levels of molybdases (NR, AO, SO, XDH) and molybdate transporters in tobacco, whereby the gene expression levels of NR were upregulated by 28.48%, 52.51%, 173.05%, and 246.21%, respectively; and MOT1 and MOT2 were upregulated by 21.49/8.67%, 66.05/30.44%, 93.05/93.26%, and 166.11/114.29%, respectively. Additionally, Mo treatment regulated the synthesis of related enzymes, effectively promoted plant growth, and significantly increased biomass and dry matter accumulation, with the biomass in the leaves increasing significantly by 30.73%, 40.72%, 46.34%, and 12.88%, respectively. The FT-NIR spectroscopy results indicate that after Mo was applied to the soil, the quantity of C-O-C, -COOH, C-H, and N-H functional groups increased. Concurrently, the contents of cellulose, hemicellulose, lignin, protopectin, and soluble pectin in the leaves significantly increased, wherein the content of soluble pectin and hemicellulose increased significantly by 31.01/288.82%, 40.69/343.43%, 69.93/241.73%, and 196.88/223.26%, respectively. Furthermore, the cell walls thickened, increasing the ability of the plant to withstand disturbances. The metabolic network diagrams indicate that Mo regulated galactose metabolism, and arginine and proline acid biosynthesis. The contents of carbohydrates, spermidine, proline, quinic acid, IAA, flavonoids, and other substances were increased, increasing the levels of polysaccharides and pectin within the cell wall, controlling lignin production, and successfully enhancing resistance to abiotic stress. These results offer important perspectives for further investigations into the role of trace elements.
NLRP3 Inflammasome-Mediated Osteoarthritis: The Role of Epigenetics
Authors: Liu Yuzhou , Wang Ying , Yan Ping , Cui Ning , Xu Kejin , Liu Da , Tian Yuan , Cao Lingling
Published: January 2025

The prevalence of osteoarthritis (OA) notably surges with age and weight gain. The most common clinical therapeutic drugs are painkillers, yet they cannot impede the deteriorating course of OA. Thus, understanding OA’s pathogenesis and devising effective therapies is crucial. It is generally recognized that inflammation, pyroptosis, and OA progression are tightly linked. The activation of NLRP3 inflammasome can lead to the discharge of the pro-inflammatory cytokines Interleukin-1β and IL-18, intensifying subsequent inflammatory reactions and promoting OA development. Conversely, the imbalance caused by deacetylase-regulated NLRP3 inflammasome underlies the chronic mild inflammation related to degenerative diseases. Therefore, this article expounds on the mechanism of OA pathogenesis and the role of histone deacetylases (HDACs) in NLRP3 inflammasome-triggered OA, and illustrates the application of HDAC inhibitors in OA, striving to provide more insights into novel OA treatment approaches.
Advances and Challenges in Pediatric Sepsis Diagnosis: Integrating Early Warning Scores and Biomarkers for Improved Prognosis
Authors: Esposito Susanna , Mucci Benedetta , Alfieri Eleonora , Tinella Angela , Principi Nicola
Published: January 2025

Identifying and managing pediatric sepsis is a major research focus, yet early detection and risk assessment remain challenging. In its early stages, sepsis symptoms often mimic those of mild infections or chronic conditions, complicating timely diagnosis. Although various early warning scores exist, their effectiveness is limited, particularly in prehospital settings where accurate, rapid assessment is crucial. This review examines the roles of clinical prediction tools and biomarkers in pediatric sepsis. Traditional biomarkers, like procalcitonin (PCT), have improved diagnostic accuracy but are insufficient alone, often resulting in overprescription of antibiotics or delayed treatment. Combining multiple biomarkers has shown promise for early screening, though this approach can be resource-intensive and less feasible outside hospitals. Predicting sepsis outcomes to tailor therapy remains underexplored. While serial measurements of traditional biomarkers offer some prognostic insight, their reliability is limited, with therapeutic decisions often relying on clinical judgment. Novel biomarkers, particularly those identifying early organ dysfunction, hold potential for improved prognostic accuracy, but significant barriers remain. Many are only available in hospitals, require further validation, or need specialized assays not commonly available, limiting broader clinical use. Further research is needed to establish reliable protocols and enhance the clinical applicability of these tools. Meanwhile, a multifaceted approach that combines clinical judgment with existing tools and biomarkers remains essential to optimize pediatric sepsis management, improving outcomes and minimizing risks.
iNOS-Produced Nitric Oxide from Cancer Cells as an Intermediate of Stemness Regulation by PARP-1 in Colorectal Cancer
Authors: del Moral-Martinez María , Sánchez-Uceta Paula , Clemente-Gonzalez Ruben , Moreno-SanJuan Sara , Puentes-Pardo Jose D. , Khaldy Huda , Lopez-Perez David , Arnedo Javier , Casado Jorge , Martínez-Heredia Luis , Carazo Angel , León Josefa
Published: January 2025

PARP-1 has been linked to the progression of several types of cancer. We have recently reported that PARP-1 influences tumor progression in CRC through the regulation of CSCs in a p53-dependent manner. In this study, we propose that nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) could act as a mediator. We evaluated the expression of iNOS in a cohort of patients previously used to analyze the effects of PARP-1 on CRC in relation to p53 status. We also developed an in vitro model in which PARP-1 was stably overexpressed. In CRC patients, iNOS expression correlated with the differentiation grade, and with a high expression of CSC markers, although only in wild-type p53 tumors, as previously found for PARP-1. In vitro, overexpression of PARP-1 induced increased growth and stemness in wild-type p53 cells, while exerting the opposite effect on mutated ones, as expected. Treatment with 1400 W, a selective inhibitor of iNOS, or gene silencing of the gene counteracted the effects of PARP-1 in both p53 wild-type and p53 mutated cells. Given that the development of resistance has been demonstrated after treatment with PARP-1 inhibitors, iNOS could be considered a new therapeutic target in CRC, although only in patients with wild-type p53 tumors.
Quantification of Myocardial Biomarkers in Sudden Cardiac Deaths Using a Rapid Immunofluorescence Method for Simultaneous Biomarker Analysis
Authors: Sacco Matteo Antonio , Aquila Valerio Riccardo , Gualtieri Saverio , Raffaele Roberto , Verrina Maria Cristina , Tarda Lucia , Gratteri Santo , Aquila Isabella
Published: January 2025

Background/Objectives: Differential diagnosis of sudden cardiac death (SCD) remains challenging, particularly in cases lacking evident structural abnormalities. Cardiac markers have been proposed as useful tools for this differentiation in forensic contexts. However, key issues include the influence of postmortem interval (PMI) on marker stability and the limitations of traditional approaches that focus on pericardial fluid, which requires invasive sampling compared to peripheral blood. This study aimed to evaluate the potential of cardiac markers in peripheral blood for diagnosing SCD, addressing methodological concerns related to PMI, hemolysis, and sample handling. Methods: This study analyzed 5 cardiac markers (creatine kinase-MB [CK-MB], myoglobin, troponin I [TnI], BNP, and D-dimer) in peripheral blood samples from 42 autopsied cadavers, divided into an SCD group and a control group. Marker levels were quantified using immunofluorescence, with cases meticulously selected to exclude confounding factors such as chronic diseases, pulmonary thromboembolism, and drowning. The study also accounted for potential degradation due to PMI, and evaluated the accuracy of point-of-care testing (POCT) in forensic samples. Results: The study identified statistically significant differences in myoglobin and TnI levels between the SCD group and the control group, though myoglobin’s diagnostic reliability remains limited due to its lack of specificity for myocardial injury. TnI emerged as a more robust marker for SCD. Contrary to prior concerns, PMI showed no significant correlation with marker levels in samples handled without freeze–thaw cycles. Issues related to hemolysis were addressed, and no significant effects were observed from resuscitation maneuvers. Conclusions: This study supports the potential use of cardiac markers, particularly TnI, in peripheral blood for postmortem SCD diagnosis, emphasizing the importance of rapid and systematic analysis to minimize hemolysis-related variability. While further validation is needed to confirm these findings, this approach offers a less invasive, economical, and practical method for forensic investigations.
Outcomes of Brain Metastasis from Lung Cancer
Authors: Mobley James M. , Phillips Kerry I. , Chen Quan , Reusch Ellen , Reddy Niharika , Magsam Julia B. , McLouth Laurie E. , Huang Bin , Villano John L.
Published: January 2025

Background/Objectives: Little information has been published on patients diagnosed with brain metastasis secondary to lung cancer. Correlating outcome patterns (hospice care, lost to follow-up, death before hospice care or treatment) and specific characteristics of treated and untreated patients may identify subsets of patients who may benefit from treatment. Methods: We evaluated data from the Kentucky Cancer Registry and identified 284 cases who were diagnosed with brain metastasis secondary to non-small cell lung cancer (NSCLC) between 1 August 2016, and 31 December 2019. We evaluated type and timing of treatment received, as well as focused on those patients who did not receive treatment. For those patients who did not receive treatment, various characteristics that may have impacted their decision or ability to undergo follow-up were also evaluated. This included social history, disease burden, as well as oncology treatment timelines. Lastly, due to the high smoking rate in Kentucky, we conducted an analysis of patient tobacco use. Results: Our results show that 61 cases (21.8%) never received treatment for lung cancer with brain metastasis. Further analysis of the non-treated cases demonstrated that 19 cases (31.1%) never met with an oncology team while in the hospital or after discharge; 14 of the 61 cases (23.0%) were too sick to receive treatment and died prior to having the option of treatment; and 47 of the 61 cases (77.0%) may have had the option of treatment but declined. Conclusions: Historically, patients with brain metastases have faced poor prognoses and limited treatment options. However, advancements in systemic chemoimmunotherapy and targeted therapies have introduced new treatment possibilities, offering improved symptom control and the potential for prolonged survival. This analysis is crucial for identifying potential barriers to care, optimizing resource allocation, and guiding future research.
On-Site Detection of Ca and Mg in Surface Water Using Portable Laser-Induced Breakdown Spectroscopy
Authors: Wan Yuanxin , Ma Shixiang , Zheng Peichao , Zhao Xiande , Xing Zhen , Jiao Leizi , Tian Hongwu , Dong Daming
Published: January 2025

Ca and Mg are key constituents in surface water that are essential nutrients and vital indicators of water hardness. Rapid on-site measurement of Ca and Mg concentrations in surface water is important. However, traditional laboratory detection methods are complex and time-consuming, and on-site detection is difficult. In this study, a portable surface water detection method was developed using laser-induced breakdown spectroscopy with a miniaturized spectrometer LIBS and a liquid jet device for sample introduction. The device enables the rapid online in situ measurement of elemental concentrations in the water. The limits of detection for the rapid on-site detection of Ca and Mg in surface water were 11.58 and 2.57 mg/L, respectively. We applied this method to assess the concentrations of Ca and Mg in authentic water samples collected from rivers and ponds. The recovery rates for Ca and Mg were 90.83–101.74% and 93.43–108.74%, respectively. This method is suitable for rapid, on-site, and highly sensitive monitoring of Ca and Mg concentrations in the environment.
Cross-Sectional Analysis of IL-6, TNF-α, Adiponectin, Leptin, and Klotho Serum Levels in Relation to BMI Among Overweight and Obese Children Aged 10–14 in La Rioja, Spain
Authors: Fernández-Vallejo Beatriz , Monteagudo Francisco Jiménez , Romero Lourdes , Aznárez Maria Isabel López , Cobas María del Carmen Romero , Pérez-Martínez Laura
Published: January 2025

Background: Childhood obesity is a major public health concern, being linked to an increased risk of metabolic disorders and cardiovascular disease. Even in childhood, obesity is associated with systemic low-grade inflammation, which is a critical factor in the development of atherosclerosis and a predictor of cardiovascular morbidity and mortality. Objectives: To describe the prevalence of obesity and examine the relationship between IL-6, TNF-α, adiponectin, leptin, the leptin/adiponectin (L/A) ratio, and Klotho levels with BMI in children. Methods: This cross-sectional study included children aged 10–14 years from La Rioja, Spain. Participants were selected based on BMI criteria for overweight (85th–95th percentiles) and obesity (>95th percentile). Socio-demographic and anthropometric data and blood samples were collected and analyzed for IL-6, TNF-α, adiponectin, leptin, and Klotho. Results: A total of 340 participants were included, with 276 (81.2%) classified as normal weight and 64 (18.8%) as overweight or obese. Mean age was similar between groups (p = 0.40). Obesity was more prevalent in males (59.4%, p = 0.048). Obese participants had higher mean birth weight (p = 0.003), current height (p = 0.04), BMI (p < 0.0001), and abdominal circumference (p < 0.0001). BMI correlated positively with leptin (r = 0.54, p = 0.0008) and the L/A ratio (r = 0.40, p = 0.025), showing sex-specific differences. Conclusions: This study underscores leptin and the L/A ratio as potential biomarkers of metabolic dysregulation in childhood obesity, particularly in females. Longitudinal studies are needed to confirm these findings and assess the clinical utility of these biomarkers in pediatric obesity management.
A Narrative Review of Biomarkers and Imaging in the Diagnosis of Acute Aortic Syndrome
Authors: Arslan Ümit , Jalalzai Izatullah
Published: January 2025

Acute aortic syndrome (AAS) encompasses a range of life-threatening conditions, including classical dissection, intramural hematoma, and penetrating aortic ulcer. Each of these conditions presents distinct clinical characteristics and carries the potential to progress to rupture. Because AAS can be asymptomatic or present with diverse symptoms, its diagnosis requires clinical evaluation, risk scoring, and biomarkers such as D-dimer (DD), C-reactive protein (CRP), homocysteine, natriuretic peptides (BNP), and imaging modalities like computed tomography (CT), magnetic resonance imaging (MRI), and echocardiography. While this review primarily focuses on widely used and clinically accessible biomarkers and imaging techniques, it also discusses alternative biomarkers proposed for diagnostic use. Although CT remains the gold standard for diagnosis, biomarkers facilitate rapid risk stratification, complementing imaging techniques. Emerging technologies, such as metabolomics, are reshaping diagnostic algorithms. Despite advances in diagnostic methods, challenges such as misdiagnosis and missed diagnoses persist. Ongoing research into novel biomarkers and innovative imaging techniques holds promise for improving diagnostic accuracy and patient outcomes.
Analyzing the Impact of Geoenvironmental Factors on the Spatiotemporal Dynamics of Forest Cover via Random Forest
Authors: Habeeb Hendaf N. , Mustafa Yaseen T.
Published: January 2025

Understanding the dynamic relationships between geoenvironmental factors and forest vegetation cover is crucial for effective forest management and planning. This study investigates the spatiotemporal dynamics of forest cover in the Duhok District in the Kurdistan Region of Iraq over a decade (2013–2023), emphasizing the impact of geoenvironmental factors via Random Forest algorithms and Landsat data. This research integrates datasets including fractional vegetation cover (FVC), groundwater levels, climate data, topography, and soil moisture data, offering a comprehensive analysis of the factors influencing forest cover. The results show that in 2013, altitude and rainfall were the primary factors influencing FVC, with areas of higher altitudes and adequate rainfall exhibiting up to 30% denser forest cover. By 2023, soil moisture and groundwater levels had emerged as the dominant factors, with soil moisture levels accounting for 25% of the variation in FVC. This shift underscores the increasing importance of water management strategies to maintain forest health. The Random Forest model demonstrated high predictive accuracy, achieving an R2 value of 0.918 (RMSE of 0.016 and MAE of 0.013) for 2013 and 0.916 (RMSE of 0.018 and MAE of 0.014) for 2023, underscoring the model’s robustness in handling nonlinear ecological processes. This study’s insights are crucial for guiding sustainable forest management practices and assisting decision-makers in formulating strategies for resource management, environmental preservation, and future planning. This study underscores the necessity of adaptive management strategies that consider evolving climatic and hydrological conditions, emphasizing continuous monitoring and advanced technologies to ensure the resilience of forest ecosystems.
Approaching Four Years of Encyclopedia
Authors: Barretta Raffaele , Wee Colin
Published: January 2025

The Encyclopedia journal, released in March 2021, will celebrate its fourth anniversary next year [...]
Recreating Place: Charles Fothergill and the Limits of Travel Writing
Authors: Perkins Pam
Published: January 2025

In 1806, Charles Fothergill, a young man with a strong interest in natural history, set out on a seven-month tour of Orkney and Shetland. His goal was to write a book about the islands that would emulate the work produced by the earlier traveller Thomas Pennant on Wales and mainland Scotland. Despite his ambition, Fothergill never succeeded in completing his book. His surviving manuscripts, which range from a rough working journal covering one part of his journey to some comments on botany that seem ready to go to press, suggest some of the difficulties that he might have found both in constructing a coherent narrative of his travels and in recreating a version of Pennant’s antiquarian and scientific travels at a time when tastes in travel writing were shifting to focus more on the pleasures of landscape and aesthetics.
Effects of Acute Lateral Ankle Sprain on Spinal Reflex Excitability and Time-to-Boundary Postural Control in Single-Leg Stance
Authors: Kim Joosung , Kim Kyung-Min
Published: January 2025

Background/Objectives: Acute lateral ankle sprain (ALAS) affects balance, often assessed by changes in traditional center of pressure (COP) parameters. Spatiotemporal measures of COP and time-to-boundary (TTB) analysis may offer improved sensitivity in detecting postural deviations associated with ALAS. However, the neurophysiological mechanism underlying these changes remains unknown. This study aimed to explore the effects of ALAS on spinal reflex excitability in the fibularis longus (FL) during single-leg balance and TTB parameters following ALAS. Methods: Fourteen participants with and without ALAS were recruited within 14 days from the onset of the injury. We assessed FL spinal reflex excitability and postural control during a single-leg stance. The primary outcomes included the H/M ratio, H-latency, and TTB parameters. For H-reflex testing, the peripheral electrical stimulation was delivered at the sciatic nerve before bifurcating into the tibial and common fibular nerve while participants maintained a single-leg balance position with the involved side of the limb. The TTB parameters of the medial–lateral (ML) and anterior–posterior (AP) directions of the mean, SD, and minimum were assessed, which indicate postural correction and strategies. Results: Patients with ALAS had a significantly lower AP-TTB minimum compared with healthy uninjured controls, with a moderate effect size (p = 0.039; d = −0.83). However, there was no significant difference in the H/M ratio (ALAS: 0.29 ± 0.16 vs. CON: 0.24 ± 0.10; p = 0.258) and H-reflex latency (ALAS: 34.6 ± 1.92 vs. CON: 33.8 ± 1.75 ms; p = 0.277); Conclusions: These results indicate that reflex control at the spinal level may have a minimal role in response to balance deficits following ALAS.
Global Tomato Production: Price Sensitivity and Policy Impact in Mexico, Türkiye, and the United States
Authors: Govindasamy Ramu , Ceylan Rahmiye Figen , Özkan Burhan
Published: January 2025

Tomato, a vital subtropical vegetable crop, is in demand globally but is produced in limited regions. Recently, its supply has become increasingly influenced by internal and external production factors. This study analyzed the impact of price fluctuations and evolving agricultural support schemes on tomato production in three key producers: Mexico, Türkiye, and the United States, which play significant roles in the global market with specialized production and trade. Using time-series price response data from 1991 to 2022, the research examined market prices, government support policies, and international trade agreements. Long-term price effects were similar in Türkiye and the USA but negligible in Mexico. Short-term price differences were positive across all countries, with the strongest impact in the USA. Financial support programs increased supplies in alignment with time-based effects. Deviations from long-term equilibrium were corrected in all countries, with Türkiye showing the fastest recovery. The results suggest that decoupled supports positively influence supply and merit further promotion.
Diploid Ancestor Tracing of Allopolyploid Cultivars in Camellia reticulata Based on ITS and RPB2 Sequences
Authors: Fan Zhifeng , Zheng Wei , Yan Chengmin , Xu Xiaodan
Published: January 2025

Camellia reticulata is a well-known ornamental species with a type specimen that is an allohexaploid, possibly descended from diploid ancestors like C. saluenensis, C. pitardii, and diploid C. reticulata. With over 1500 years of cultivation, heritage C. reticulata cultivars show varied ploidy levels, including hexaploid, octoploid, and decaploid forms, though their diploid ancestors are still unidentified. This study aims to trace these diploid ancestors by cloning and sequencing ITS from 25 taxa and RPB2 from 21 taxa across various ploidy levels of C. reticulata and its traditional cultivars and related species, combined with their fruit morphology data. Analyses of the ITS and RPB2 sequences suggest that the diploid ancestors of C. reticulata and its traditional cultivars may include C. saluenensis, C. pitardii, and diploid C. reticulata, while excluding C. mairei and C. polyodonta. Morphological analysis showed that diploid C. reticulata has significantly larger fruit weight, diameter, and pericarp thickness compared to C. pitardii, C. saluenensis, and both tetraploid and hexaploid C. reticulata. Since diploid ancestors of allopolyploids are often classified as distinct taxa, we suggest that diploid C. reticulata be recognized as a new variety of C. pitardii, as its ITS sequences are closely aligned with C. pitardii. This study offers key insights into the origin, evolution, and breeding of C. reticulata.
Athletes’ Knowledge of Pelvic Floor Dysfunction and Their Knowledge of and Engagement with Pelvic Floor Muscle Training: A Scoping Review
Authors: Magor Jacinta , Martin Romany , Bird Marie-Louise
Published: January 2025

Introduction: Pelvic floor dysfunction (PFD) is prevalent among athletes. Investigating whether athletes are practicing pelvic floor muscle training (PFMT) will assist in delineating the factors underlying the burden of PFD in this population. Additionally, investigating athletes’ knowledge of PFD and knowledge of and attitudes toward PFMT may inform interventions to improve the practice of PFMT. Aims: This scoping review aimed to collate the available evidence regarding athletes’ knowledge of PFD and their knowledge of and engagement with PFMT. Furthermore, this review aimed to determine the types of athletes (sports and competition levels) research had been conducted on and the definitions of PFD and PFMT most used. Methods: Six databases were searched up to January 2024. No study design or publication types were restricted; however, non-English articles were excluded due to resource constraints. Additional publications were identified through the reference lists of included articles. Data were synthesized and presented under subheadings relevant to the aims of this review. Results: Thirty-five publications were included for data extraction. Athletes had low practice of PFMT, poor knowledge of PFD and PFMT, but positive attitudes toward PFMT. One publication reported that higher levels of knowledge were associated with a lower prevalence of PFD. The athletic population was heterogeneous in sport type and competition level, and the definitions of PFD and PFMT were ill-defined. Conclusions: Athletes have a low practice of PFMT and poor knowledge of PFD and PFMT. The education of athletes may have a role in supporting pelvic floor health.