bluuebunny/crossref_metadata_2025 huggingface dataset:
The Potential of Production Forests for Sustaining Lichen Diversity: A Perspective on Sustainable Forest Management
Authors: Lõhmus Piret , Lõhmus Asko
Published: November 2019

There is a critical gap in our knowledge about sustainable forest management in order to maintain biodiversity with respect to allocating conservation efforts between production forests and set-asides. Field studies on this question are notably scarce on species-rich, poorly detectable taxon groups. On the basis of forest lichen surveys in Estonia, we assessed the following: (i) how much production stands contribute to maintaining the full species pool and (ii) how forest habitat conditions affect this contribution for habitat specialist species. The field material was collected in a “semi-natural forestry” system, which mitigates negative environmental impacts of even-aged forestry and forestry drainage by frequently using natural regeneration, tree retention, and low intensity of thinnings. We performed standard-effort surveys of full assemblages of lichens and allied fungi (such as non-lichenized calicioid and lichenicolous fungi) and measured stand structure in 127 2 ha plots, in mainland Estonia. The plots represented four management stages (old growth, mature preharvest forests, clear-cut sites with retention trees, and clear-cut sites without retention trees). The 369 recorded species represent an estimated 70% of the full species pool studied. Our main finding was that production forests supported over 80% of recorded species, but only one-third appears tolerant of management intensification. The landscape-scale potential of production forests through biodiversity-friendly silviculture is approximately twice as high as the number of tolerant species and, additionally, many very rare species depend on setting aside their scattered localities. The potential is much smaller at the scale of individual stands. The scale effect emerges because multiple stands contribute different sets of sensitive and infrequent species. When the full potential of production forests is realized, the role of reserves is to protect specific old-growth dependent taxa (15% to 20% of the species pool). Our study highlights that production forests form a heterogeneous and dynamic target for addressing the biodiversity conservation principle of sustainable forest management.
An Analysis Applying InSAR of Subsidence Caused by Nearby Mining-Induced Earthquakes
Authors: Hejmanowski Ryszard , Malinowska Agnieszka A. , Witkowski Wojciech T. , Guzy Artur
Published: November 2019

Earthquake occurrence is usually unpredictable apart from sites in the vicinity of volcanoes. It is not easy to measure displacements caused by seismic phenomena using classical geodetic methods, which are based on point survey. Therefore, the surveying of ground movements caused by seismic events should be carried out continuously. Nowadays, remote sensing data and InSAR are often applied to monitor ground displacements in areas affected by seismicity. The effects of severe nearby mining-induced earthquakes have been discussed in the paper. The earthquakes occurred in 2017 and had a magnitude of 4.7 and 4.8. The distance between the epicenters of the mining-induced earthquakes was around 1.6 km. The aim of the investigation has been to analyze the spatio-temporal distribution of ground movements caused by the two tremors using the InSAR technique. Superposition of surface displacement has been studied in time and space. The main scientific aim has been to prove that in the areas where high-energy tremors occur, ground movements overlap. Due to proximity between the epicenters, the mining-induced earthquakes caused the formation of a large subsidence trough with the dimension of approximately 1.2 km × 4.2 km and total subsidence of ca. 116 mm. Two-time phases of subsidence were determined with temporal overlapping. The subsidence analysis has enhanced the cognition of the impact of mining-induced seismicity on the kinematics of surface changes. Moreover, the present work supports the thesis that InSAR is a valuable and adequately accurate technique to monitor ground displacements caused by mining induced earthquakes.
Evaluation of Remote-Sensing based Estimates of Actual Evapotranspiration over (Diverse Shape and Sized) Palmiet Wetlands
Authors: Ramatsabana Phatsimo , Tanner Jane , Mantel Sukhmani , Palmer Anthony , Ezenne Gloria
Published: November 2019

Accurately quantifying actual evapotranspiration (ETa) over wetlands is important for the improved management of these ecosystems, since 65% of them are threatened by clearing or drainage in South Africa. This study evaluated a range of available estimates of ETa over six palmiet wetlands, which are key ecological structures in terms of water regulation and sediment trapping. The research compared three remote sensing based products (a local product, FruitLook, and two global data products, MOD16 ET and EEFlux) across different rainfall years (2008 to 2019). Their outputs were validated, where possible, with limited ground-based scintillometer data on the Krom palmiet wetland, which indicated that MOD16 and EEFlux were most representative of ground-based measurements. We also compared the small pixel size EEFlux data over three wetlands with ETa over increasing buffers of land cover (100, 500, 1000 m) in order to validate the perception of these wetlands being high water users. While larger wetlands had slightly higher evaporative demands than adjacent areas, ETa over a small wetland was similar to neighboring land cover. The results indicate that palmiet wetland ETa is highly variable and dependent on external factors such as climate, wetland size and seasonality.
Prevention of Bone Resorption by HA/β-TCP + Collagen Composite after Tooth Extraction: A Case Series
Authors: Lin Hsi Kuei , Pan Yu Hwa , Salamanca Eisner , Lin Yu Te , Chang Wei Jen
Published: November 2019

After tooth extraction, alveolar ridge loss due to resorption is almost inevitable. Most of this bone loss occurs during the first six months after the extraction procedure. Many studies have indicated that applying socket-filling biomaterials after extraction can effectively reduce the resorption rate of the alveolar ridge. The purpose of this study was to investigate the clinical efficacy of the application of a hydroxyapatite/β-tricalcium plus collagen (HA/β-TCP + collagen) dental bone graft in dental sockets immediately after tooth extraction, so as to prevent socket resorption. The study was conducted on 57 extraction sockets located in the mandible and maxilla posterior regions in 51 patients. HA/β-TCP + collagen was inserted into all of the dental sockets immediately after extraction, and was covered with a flap. Follow-up was performed for three months after extraction, using radiographs and stents for the vertical and horizontal alveolar ridge measurements. A minimal alveolar bone width reduction of 1.03 ± 2.43 mm (p < 0.05) was observed. The height reduction showed a slight decrease to 0.62 ± 1.46 mm (p < 0.05). Radiographically, the bone height was maintained after three months, indicating a good HA/β-TCP + collagen graft performance in preserving alveolar bone. In conclusion, the HA/β-TCP + collagen graft demonstrated adequate safety and efficacy in dental socket preservation following tooth extraction.
The Association of Environmental Tobacco Smoke Exposure and Inflammatory Markers in Hospitalized Children
Authors: Mahabee-Gittens E. Melinda , Merianos Ashley L. , Fulkerson Patricia C. , Stone Lara , Matt Georg E.
Published: November 2019

Background: Environmental tobacco smoke (ETS) exposure is associated with altered cytokine levels in children. We sought to examine ETS exposure prevalence and the relationship between ETS exposure and cytokine levels in a sample of hospitalized children. (2) Methods: Inflammatory markers (IL-8, IL-1β, IL-10, and TNF-α) and cotinine were measured in saliva of hospitalized, nonsmoking children (N = 112). To assess the association between ETS exposure and immune system response, we built a multivariate regression model including the four inflammatory markers as the response variables and cotinine, age, sex, and discharge diagnosis as explanatory variables while assessing possible interaction effects. (3) Results: Mean age (SD) was 5.8(5.0) years; Geometric Mean (GeoM) cotinine = 1.8 [95% CI = 1.4–2.2]. Children with non-inflammatory other diagnoses had lower IL-10 (p = 0.003) and TNF-α (p = 0.009) levels than children with inflammatory other diagnoses. Children with asthma (p = 0.01) and bacterial illnesses and/or pneumonia (p = 0.002) had higher IL-8 levels. Independent of diagnosis, there was a significant curvilinear association between cotinine and IL-1β (p = 0.002) reflecting no association for cotinine levels <5 ng/mL and a positive association for >5 ng/mL. (4) Conclusions: Children with higher ETS exposure levels have higher IL-1β levels regardless of age, sex, and diagnosis. ETS exposure may increase pro-inflammatory immune responses in children and may interfere with native immune responses and the ability to heal and fight infection.
Exit and Entry Screening Practices for Infectious Diseases among Travelers at Points of Entry: Looking for Evidence on Public Health Impact
Authors: Mouchtouri Varvara A. , Christoforidou Eleni P. , an der Heiden Maria , Menel Lemos Cinthia , Fanos Margherita , Rexroth Ute , Grote Ulrike , Belfroid Evelien , Swaan Corien , Hadjichristodoulou Christos
Published: November 2019

A scoping search and a systematic literature review were conducted to give an insight on entry and exit screening referring to travelers at points of entry, by analyzing published evidence on practices, guidelines, and experiences in the past 15 years worldwide. Grey literature, PubMed. and Scopus were searched using specific terms. Most of the available data identified through the systematic literature review concerned entry screening measures at airports. Little evidence is available about entry and exit screening measure implementation and effectiveness at ports and ground crossings. Exit screening was part of the World Health Organisation’s (WHO) temporary recommendations for implementation in certain points of entry, for specific time periods. Exit screening measures for Ebola Virus Disease (EVD) in the three most affected West African countries did not identify any cases and showed zero sensitivity and very low specificity. The percentages of confirmed cases identified out of the total numbers of travelers that passed through entry screening measures in various countries worldwide for Influenza Pandemic (H1N1) and EVD in West Africa were zero or extremely low. Entry screening measures for Severe Acute Respiratory Syndrome (SARS) did not detect any confirmed SARS cases in Australia, Canada, and Singapore. Despite the ineffectiveness of entry and exit screening measures, authors reported several important concomitant positive effects that their impact is difficult to assess, including discouraging travel of ill persons, raising awareness, and educating the traveling public and maintaining operation of flights from/to the affected areas. Exit screening measures in affected areas are important and should be applied jointly with other measures including information strategies, epidemiological investigation, contact tracing, vaccination, and quarantine to achieve a comprehensive outbreak management response. Based on review results, an algorithm about decision-making for entry/exit screening was developed.
Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates
Authors: Miao Lingzhan , Guo Song , Liu Zhilin , Liu Songqi , You Guoxiang , Qu Hao , Hou Jun
Published: November 2019

Nanoplastic (NP) contamination is becoming a pervasive issue as NPs, originating from microplastic particles, pose potentially harmful environmental impacts on aquatic ecosystems. The environmental hazards of NPs on microorganisms have been well documented in recent studies; however, little is known about their ecotoxicity effects on freshwater biofilms, which serve as important primary producers and decomposers and are highly connected with other ecosystem components. We investigated the effects of NPs on the microbial metabolic functions of freshwater biofilms in terms of carbon source utilization ability. Biofilm samples were collected, cultivated in a hydrodynamic flume for six weeks, and then exposed in polystyrene (PS) beads (100 nm in size) with different NP concentrations (1, 5, and 10 mg/L). BIOLOG ECO microplates were used to quantify carbon source utilization characteristics. The data were analyzed using average well-color development (AWCD), functional diversity indices, and principle component analysis (PCA). Results showed that the total carbon metabolic functions (represented by AWCD) remained constant (p > 0.05) with elevated NP concentrations, but some specific carbon sources (e.g., esters) changed in their utilization ability (p < 0.05). The microbial functional diversity (Shannon–Wiener diversity index, Simpson diversity index, and Shannon evenness index) was significantly reduced under 10 mg/L NPs (p < 0.05), indicating an inhibiting effect of NPs on biofilm metabolic diversity. This study examined NP ecotoxicity effects on microbial metabolic activities at the community level, but further studies are required to fully understand the mechanisms driving this change.
Spatiotemporal Analysis and Control of Landscape Eco-Security at the Urban Fringe in Shrinking Resource Cities: A Case Study in Daqing, China
Authors: Chen Xi , Xu Dawei , Fadelelseed Safa , Li Lianying
Published: November 2019

As the main bearing area of the ecological crisis in resource-rich cities, it is essential for the urban fringe to enhance regional ecological security during a city’s transformation. This paper takes Daqing City, the largest oilfield in China’s cold land, as an example. Based on remote sensing image data from 1980 to 2017, we use the DPSIR (Driving forces, Pressure, State, Impact, Response) framework and spatial auto-correlation analysis methods to assess and analyze the landscape eco-security change of the study area. From the perspective of time–space, the study area is partitioned, and control strategies are proposed. The results demonstrate that: (1) The landscape eco-security changes are mainly affected by oilfield exploitation and ecological protection policies; the index declined in 1980–2000 and increased in 2000–2017. (2) The landscape eco-security index has obvious spatial clustering characteristics, and the oil field is the main area of warning. (3) The study area determined the protection area of 1692.07 km2, the risk restoration area of 979.64 km2, and proposed partition control strategies. The results are expected to provide new decision-making ideas in order to develop land use management and ecological plans for the management of Daqing and other resource shrinking cities.
A Scientometric Review of Resource Recycling Industry
Authors: Wang Minxi , Liu Ping , Gu Zhaoliang , Cheng Hong , Li Xin
Published: November 2019

With rapid economic development and urbanization, a large number of primary resources are consumed and accumulate in society as recyclable resource, which causes great pressure on the environment. The development of the resource recycling industry (RRI) can reduce environmental impacts and achieve sustainable development and green growth. Scholars are paying more attention to the resource recycling industry (RRI), and the related literature continues to increase. There are over 7041 publications covering RRI in the Web of Science database from 1996 to 2018. This paper analyzes the time distribution characteristics of the literature and the status of the scientific research cooperation network using the visualization analysis software CiteSpace. The number of documents increased from 94 in 1996 to a peak of 963 in 2018. There is no relatively stable core author group. The number of papers published by “Chinese Acad Sci” ranks first among all research institutions. Document co-citation analysis and burst detection are adopted to assess the status and emerging trends in the RRI research domain. A publication by M.C. Monte on waste management is the most cited paper. Additionally, “green and sustainable and technology” and “science and technology—other topics” are the latest emerging subject categories in RRI research. Furthermore, “e-waste”, “reverse logistics” and “lean manufacturing” are emerging research trends for RRI, and “carbon emissions”, “policy”, “demolition waste”, “supply chain management” and “compressive strength” have become hot topics. These findings may provide inspiration for scholars to search for new research directions and ideas.
Trends in Overweight and Obesity Among Children and Adolescents in China from 1991 to 2015: A Meta-Analysis
Authors: Guo Yaru , Yin Xiaojian , Wu Huipan , Chai Xiaojiang , Yang Xiaofang
Published: November 2019

This meta-analysis of overweight and obesity (ow/ob) among children and adolescents in China from 1991 to 2015 provides a reference for promoting the healthy development of children and adolescents. The studies were retrieved from the China National Knowledge Infrastructure, Wanfang, and PubMed databases for the period from January 1991 to January 2018. The data were combined and analyzed, and the combined effect magnitude odds ratio and the 95% confidence interval were calculated. Publication bias was determined using Stata/SE12.0. We found that (1) the prevalence of ow/ob increased from 5.0% and 1.7% in 1991–1995 to 11.7% and 6.8% in 2011–2015, respectively, and the overweight rate was the greatest in 2006–2010; (2) from 1991 to 2015, the prevalence of ow/ob was greater in urban areas than in rural areas; (3) compared with girls, boys were more likely to be ow/ob; and (4) the prevalence rates of ow and ob were greater in infancy than in other growth stages, with values of 11.7% and 7.0%, respectively. The prevalence of ow/ob among Chinese children and adolescents showed significant differences based on region, sex, and age. An overall upward trend was observed that decreased slightly from 2011 to 2015.
Cellular Processes Involved in Jurkat Cells Exposed to Nanosecond Pulsed Electric Field
Authors: Li Huijuan , Liu Shibin , Yang Xue , Du Yongqian , Luo Jiezhang , Tan Jie , Sun Yulong
Published: November 2019

Recently, nanosecond pulsed electric field (nsPEF) has been considered as a new tool for tumor therapy, but its molecular mechanism of function remains to be fully elucidated. Here, we explored the cellular processes of Jurkat cells exposed to nanosecond pulsed electric field. Differentially expressed genes (DEGs) were acquired from the GEO2R, followed by analysis with a series of bioinformatics tools. Subsequently, 3D protein models of hub genes were modeled by Modeller 9.21 and Rosetta 3.9. Then, a 100 ns molecular dynamics simulation for each hub protein was performed with GROMACS 2018.2. Finally, three kinds of nsPEF voltages (0.01, 0.05, and 0.5 mV/mm) were used to simulate the molecular dynamics of hub proteins for 100 ns. A total of 1769 DEGs and eight hub genes were obtained. Molecular dynamic analysis, including root mean square deviation (RMSD), root mean square fluctuation (RMSF), and the Rg, demonstrated that the 3D structure of hub proteins was built, and the structural characteristics of hub proteins under different nsPEFs were acquired. In conclusion, we explored the effect of nsPEF on Jurkat cell signaling pathway from the perspective of molecular informatics, which will be helpful in understanding the complex effects of nsPEF on acute T-cell leukemia Jurkat cells.
IL-33/IL-31 Axis in Immune-Mediated and Allergic Diseases
Authors: Murdaca Giuseppe , Greco Monica , Tonacci Alessandro , Negrini Simone , Borro Matteo , Puppo Francesco , Gangemi Sebastiano
Published: November 2019

Several allergic and immunologic diseases including asthma, food allergy (FA), chronic spontaneous urticaria (CSU), atopic dermatitis (AD), systemic lupus erythematosus (SLE), systemic sclerosis (SSc), rheumatoid arthritis (RA), and Behçet’s disease (BD) are characterized by the involvement of Th2 immunity. Several mediators lead to immunoglobulin (Ig)E production, thus including key cytokines such as interleukin (IL)-4, IL-5, and IL-13. Among them, IL-31 and IL-33 have been recently studied as novel biomarkers and future therapeutic targets for allergic and immunological disorders. IL-31 is a proinflammatory cytokine—it regulates cell proliferation and is involved in tissue remodeling. IL-33, acting through its receptor suppression of tumorigenity (ST2L), is an alarmin cytokine from the IL-1 family, whose expression is mediated by tissue damage. The latter has a pleiotropic effect, as it may modulate specific and innate immune cells functions. To date, several researchers have investigated the involvement of IL-31 and IL-33 in several allergic and immune-mediated diseases. Further studies are needed to understand the future applications of these molecules as novel therapeutic agents. This paper aims to give the readers a complete and updated review of IL-31 and IL-33 involvement among the most common autoimmune and allergic disorders.
Impact of Carbon Nanomaterials on the Antioxidant System of Tomato Seedlings
Authors: González-García Yolanda , López-Vargas Elsy Rubisela , Cadenas-Pliego Gregorio , Benavides-Mendoza Adalberto , González-Morales Susana , Robledo-Olivo Armando , Alpuche-Solís Ángel Gabriel , Juárez-Maldonado Antonio
Published: November 2019

Tomato is one of the most economically important vegetables worldwide and is constantly threatened by various biotic and abiotic stress factors reducing the quality and quantity in the production of this crop. As an alternative to mitigate stress in plants, carbon nanomaterials (CNMs) have been used in agricultural areas. Therefore, the objective of the present work was to evaluate the antioxidant responses of tomato seedlings to the application via foliar and drench of carbon nanotubes (CNTs) and graphene (GP). Different doses (10, 50, 100, 250, 500, and 1000 mg L−1) and a control were evaluated. The results showed that the fresh and dry root weight increased with the application of CNMs. Regarding the antioxidant responses of tomato seedlings, the application of CNMs increased the content of phenols, flavonoids, ascorbic acid, glutathione, photosynthetic pigments, activity of the enzyme’s ascorbate peroxidase, glutathione peroxidase, catalase, and phenylalanine ammonia lyase as well as the content of proteins. Therefore, the use of carbon-based nanomaterials could be a good alternative to induce tolerance to different stress in tomato crop.
MicroRNA-29a Exhibited Pro-Angiogenic and Anti-Fibrotic Features to Intensify Human Umbilical Cord Mesenchymal Stem Cells—Renovated Perfusion Recovery and Preventing against Fibrosis from Skeletal Muscle Ischemic Injury
Authors: Su Wen-Hong , Wang Ching-Jen , Hung Yi-Yung , Lu Chun-Wun , Ou Chia-Yu , Tseng Shun-Hung , Tsai Ching-Chin , Kao Yun-Ting , Chuang Pei-Chin
Published: November 2019

This study was conducted to elucidate whether microRNA-29a (miR-29a) and/or together with transplantation of mesenchymal stem cells isolated from umbilical cord Wharton’s jelly (uMSCs) could aid in skeletal muscle healing and putative molecular mechanisms. We established a skeletal muscle ischemic injury model by injection of a myotoxin bupivacaine (BPVC) into gastrocnemius muscle of C57BL/6 mice. Throughout the angiogenic and fibrotic phases of muscle healing, miR-29a was considerably downregulated in BPVC-injured gastrocnemius muscle. Overexpressed miR-29a efficaciously promoted human umbilical vein endothelial cells proliferation and capillary-like tube formation in vitro, crucial steps for neoangiogenesis, whereas knockdown of miR-29a notably suppressed those endothelial functions. Remarkably, overexpressed miR-29a profitably elicited limbic flow perfusion and estimated by Laser Dopple. MicroRNA-29a motivated perfusion recovery through abolishing the tissue inhibitor of metalloproteinase (TIMP)-2, led great numbers of pro-angiogenic matrix metalloproteinases (MMPs) to be liberated from bondage of TIMP, thus reinforced vascular development. Furthermore, engrafted uMSCs also illustrated comparable effect to restore the flow perfusion and augmented vascular endothelial growth factors-A, -B, and -C expression. Notably, the combination of miR29a and the uMSCs treatments revealed the utmost renovation of limbic flow perfusion. Amplified miR-29a also adequately diminished the collagen deposition and suppressed broad-wide miR-29a targeted extracellular matrix components expression. Consistently, miR-29a administration intensified the relevance of uMSCs to abridge BPVC-aggravated fibrosis. Our data support that miR-29a is a promising pro-angiogenic and anti-fibrotic microRNA which delivers numerous advantages to endorse angiogenesis, perfusion recovery, and protect against fibrosis post injury. Amalgamation of nucleic acid-based strategy (miR-29a) together with the stem cell-based strategy (uMSCs) may be an innovative and eminent strategy to accelerate the healing process post skeletal muscle injury.
Transcriptome Profiling Unravels a Vital Role of Pectin and Pectinase in Anther Dehiscence in Chrysanthemum
Authors: Li Qian , Wu Ze , Wu Huijun , Fang Weimin , Chen Fadi , Teng Nianjun
Published: November 2019

Chrysanthemum (Chrysanthemum morifolium (Ramat.) Kitamura) plants have great ornamental value, but their flowers can also be a source of pollen contamination. Previously, morphological and cytological studies have shown that anthers of some chrysanthemum cultivars such as ‘Qx-115′ fail to dehisce, although the underlying mechanism is largely unknown. In this study, we investigated the molecular basis of anther indehiscence in chrysanthemum via transcriptome analysis of a dehiscent cultivar (‘Qx-097′) and an indehiscent cultivar (‘Qx-115′). We also measured related physiological indicators during and preceding the period of anther dehiscence. Our results showed a difference in pectinase accumulation and activity between the two cultivars during dehiscence. Detection of de-esterified pectin and highly esterified pectin in anthers during the period preceding anther dehiscence using LM19 and LM20 monoclonal antibodies showed that both forms of pectin were absent in the stomium region of ‘Qx-097′ anthers but were abundant in that of ‘Qx-115′ anthers. Analysis of transcriptome data revealed a significant difference in the expression levels of two transcription factor-encoding genes, CmLOB27 and CmERF72, between ‘Qx-097′ and ‘Qx-115′ during anther development. Transient overexpression of CmLOB27 and CmERF72 separately in tobacco leaves promoted pectinase biosynthesis. We conclude that CmLOB27 and CmERF72 are involved in the synthesis of pectinase, which promotes the degradation of pectin. Our results lay a foundation for further investigation of the role of CmLOB27 and CmERF72 transcription factors in the process of anther dehiscence in chrysanthemum.
Different Forms of TFF2, A Lectin of the Human Gastric Mucus Barrier: In Vitro Binding Studies
Authors: Heuer Franziska , Stürmer René , Heuer Jörn , Kalinski Thomas , Lemke Antje , Meyer Frank , Hoffmann Werner
Published: November 2019

Trefoil factor family 2 (TFF2) and the mucin MUC6 are co-secreted from human gastric and duodenal glands. TFF2 binds MUC6 as a lectin and is a constituent of the gastric mucus. Herein, we investigated human gastric extracts by FPLC and identified mainly high- but also low-molecular-mass forms of TFF2. From the high-molecular-mass forms, TFF2 can be completely released by boiling in SDS or by harsh denaturing extraction. The low-molecular-mass form representing monomeric TFF2 can be washed out in part from gastric mucosa specimens with buffer. Overlay assays with radioactively labeled TFF2 revealed binding to the mucin MUC6 and not MUC5AC. This binding is modulated by Ca2+ and can be blocked by the lectin GSA-II and the monoclonal antibody HIK1083. TFF2 binding was also inhibited by Me-β-Gal, but not the α anomer. Thus, both the α1,4GlcNAc as well as the juxtaperipheral β-galactoside residues of the characteristic GlcNAcα1→4Galβ1→R moiety of human MUC6 are essential for TFF2 binding. Furthermore, there are major differences in the TFF2 binding characteristics when human is compared with the porcine system. Taken together, TFF2 appears to fulfill an important role in stabilizing the inner insoluble gastric mucus barrier layer, particularly by its binding to the mucin MUC6.
Queen Recognition Signals in Two Primitively Eusocial Halictid Bees: Evolutionary Conservation and Caste-Specific Perception
Authors: Steitz Iris , Brandt Katharina , Biefel Felix , Minat Ädem , Ayasse Manfred
Published: November 2019

Queen signals are known to regulate reproductive harmony within eusocial colonies by influencing worker behavior and ovarian physiology. However, decades of research have resulted in the identification of just a few queen signals, and studies of their mode of action are rare. Our aim was to identify queen recognition signals in the halictid bee Lasioglossum pauxillum and to analyze caste differences in the olfactory perception of queen signals in L. pauxillum and the closely related species L. malachurum. We performed chemical analyses and bioassays to test for caste differences in chemical profiles and worker behavior influenced by queen-specific compounds in L. pauxillum. Our results indicated that caste differences in the chemical profiles were mainly attributable to higher amounts of macrocyclic lactones in queens. Bioassays demonstrated a higher frequency of subordinate behavior in workers elicited by queen-specific amounts of macrocyclic lactones. Thus, macrocyclic lactones function as queen recognition signals in L. pauxillum, as in L. malachurum. Using electrophysiological analyses, we have demonstrated that queens of both tested species lack antennal reactions to certain macrocyclic lactones. Therefore, we assume that this is a mechanism to prevent reproductive self-inhibition in queens. Our results should stimulate debate on the conservation and mode of action of queen signals.
The Important Role of Immunotherapies in Acute Myeloid Leukemia
Authors: Greiner Jochen
Published: November 2019

This series on immunotherapies in acute myeloid leukemia (AML) aims to give readers new insights on established but also emerging immunotherapeutic approaches for AML patients [...]
LABAs and p38MAPK Inhibitors Reverse the Corticosteroid-Insensitivity of IL-8 in Airway Smooth Muscle Cells of COPD
Authors: Knobloch Jürgen , Jungck David , Kronsbein Juliane , Stoelben Erich , Ito Kazuhiro , Koch Andrea
Published: November 2019

Airway inflammation in chronic obstructive pulmonary disease (COPD) is partially insensitive/resistant to inhaled corticosteroids (ICS). ICS plus bronchodilator therapy has been discussed for COPD phenotypes with frequent exacerbations and participation of corticosteroid-sensitive type 2/eosinophilic inflammation. Neutralization of non-type 2/IL-8-associated airway inflammation by reversion of its corticosteroid-resistance might be a future strategy for other phenotypes. Human airway smooth muscle cells (HASMCs) produce corticosteroid-insensitive IL-8 in response to TNFα or LPS in stable disease stages or bacteria-induced exacerbations, respectively. p38-mitogen-activated-protein-kinases (p38MAPKs) are alternative therapeutic targets. Hypothesis: long-acting-β2-agonists (LABAs) reverse the corticosteroid-insensitivity of IL-8 by p38MAPK inhibition in HASMCs. Cultivated HASMCs from COPD subjects were pre-incubated with formoterol, salmeterol, fluticasone-propionate, BIRB796 (p38MAPKα, -γ, -δ inhibitor), and/or SB203580 (p38MAPKα and -β inhibitor) before stimulation with TNFα or LPS. IL-8 and MAPK-activities were measured by ELISA. Formoterol, salmeterol, and fluticasone did not or hardly reduced TNFα- or LPS-induced IL-8. BIRB796 and SB203580 reduced TNFα-induced IL-8. SB203580 reduced LPS-induced IL-8. Fluticasone/formoterol, fluticasone/salmeterol, and fluticasone/BIRB796, but not fluticasone/SB203580 combinations, reduced TNFα-induced IL-8 stronger than single treatments. All combinations including fluticasone/SB203580 reduced LPS-induced IL-8 stronger than single treatments. TNFα induced p38MAPKα and -γ activity. LPS induced p38MAPKα activity. Formoterol reduced TNFα-induced p38MAPKγ and LPS-induced p38MAPKα activity. LABAs reverse the corticosteroid-insensitivity of IL-8 in airway smooth muscles via p38MAPKγ in stable disease and via p38MAPKα in exacerbations. Our pre-clinical data indicate a utility for also adding ICS in non-type 2 inflammatory COPD phenotypes to bronchodilator therapy. Depending on phenotype and disease stage, isoform-specific p38MAPK blockers might also reverse corticosteroid-resistance in COPD.
A Study of Multi-Component Oscillating-Foil Hydrokinetic Turbines with a GPU-Accelerated Boundary Element Method
Authors: Koutsogiannakis Panagiotis E. , Filippas Evangelos S. , Belibassakis Kostas A.
Published: November 2019

A biomimetic semi-activated oscillating-foil device with multiple foils in a parallel configuration is studied for the extraction of marine renewable energy. For the present investigation, an unsteady boundary element method (BEM) is used for the simulation of 3D lifting flows. For this work, the device is assumed to be submerged far from the free surface and the sea bottom. However, the geometry of the body and the initial shape of the wake are general. For the numerical simulations, a high performance in-house GPU-accelerated code (GPU-BEM) is developed. For the calculation of singular integrals, an adaptive algorithm based on the Gauss–Lobatto quadrature is used. Concerning the numerical scheme of GPU-BEM, the convergence of the method was tested, the numerical characteristics were determined and the method was validated. A parametric study of a single-foil device is presented to determine the performance characteristics of such devices. Next, twin-foil devices are investigated in parallel and staggered configurations with a phase difference between the two foils. Finally, the multiple-foil parallel configuration is compared against turbines. After enhancement and further verification, the present method is proposed for the design and control of such biomimetic devices for the extraction of energy from waves and tidal currents nearshore.
Formal and Methodological Approaches to Applied Linguistics (Languages Special Issue)
Authors: Mujcinovic Sonja , Gómez Garzarán Eduardo
Published: November 2019

This Special Issue, entitled Formal and Methodological Approaches to Applied Linguistics, brings together recently published papers on various aspects of applied linguistics presented at the AESLA37 (Spanish Society of Applied Linguistics) conference in Valladolid held on 27–29 March 2019 [...]
Terahertz Time-Domain Reconstruction of Coating Microstratigraphy on Gilded Surfaces
Authors: Cacciari Ilaria , Ciofini Daniele , Baija Hubert , Siano Salvatore
Published: November 2019

Here, a systematic study in order to assess the potential of THz time domain reflectometry for measuring the thicknesses of overpaint layers applied on original gilded surfaces was carried out. The work is part of a thorough characterization campaign, which is going on at the Rijksmuseum for addressing the conservation problems of a set of 19th century gilded picture frames on which heavy coatings were applied in previous undocumented restoration interventions. To perform such non-invasive thickness measurements, an analytical protocol based on Gaussian fits of the THz pulse-echo temporal profiles was optimized through the preparation of suitable technical samples and the comparison with direct thickness measurements. Finally, the methodology was validated by characterizing the microstratigraphy of an original sculptural element from a gilded picture frame in the Rijksmuseum collection. The results achieved show the effectiveness of the present approach in revealing multi-layered dielectric microstructures with a spatial resolution of about 30 µm when using a spectral range up to 1.5 THz.
Effects of Microwave Sintering Temperature and Holding Time on Mechanical Properties and Microstructure of Si3N4/n-SiC ceramics
Authors: Qiao Li , Wang Zhenhua , Lu Taiyi , Yuan Juntang
Published: November 2019

The n-SiC (nanometer SiC) is added to be the additive in order to improve the mechanical performance of Si3N4 ceramics. A microwave sintered the ceramics at different temperature and holding times. The results shows that the Si3N4/n-SiC ceramics (85 wt% Si3N4 + 5 wt% n-SiC + 5 wt% Al2O3 + 5 wt% Y2O3) have the best mechanical properties at 1600 °C, which is beneficial to the densification and β-Si3N4 phase formation for 10 min: the density, hardness, and fracture toughness were 97.1%, 14.44 GPa, and 7.77 MPa·m1/2, which increased by 2.8%, 7.0%, and 13.1%, respectively, when compared with the ceramics (90 wt% Si3N4 + 5 wt% Al2O3 + 5 wt% Y2O3).
A Multiple Twin-Roller Casting Technique for Producing Metallic Glass and Metallic Glass Composite Strips
Authors: Wu Yi , Zhang Long , Chen Sen , Li Wen , Zhang Haifeng
Published: November 2019

To date it has not been possible to produce metallic glass strips with a thickness larger than 150 μm via single-roller melt spinning technique, and it remains challenging to produce thick metallic glass strips. In this work, a multiple twin-roller casting technique is proposed for producing thick metallic glass and metallic glass composite strips. A triple twin-roller casting device, as a specific case of the multiple twin-roller, was designed and manufactured. The triple twin-roller device possesses a high cooling rate and involves a long contact time between the melt and the strip, which makes it an efficient technique for producing metallic glass strips that avoids crystallization, although the solidification temperature ranges of metallic glasses are as wide as several hundred Kelvins. The two prepared metallic glass (MG) strips are in a fully amorphous state, and the MG strip shows excellent capacity of stored elastic energy under 3-point bending. Furthermore, the Ti-based metallic glass composite strip produced via the triple twin-roller casting exhibits a novel microstructure with much finer and more homogenously orientated β-Ti crystals, as compared with the microstructure of metallic glass composites produced by the common copper mold casting technique.
Lead-Free Perovskites for Lighting and Lasing Applications: A Minireview
Authors: Ushakova Elena V. , Cherevkov Sergei A. , Kuznetsova Vera A. , Baranov Alexander V.
Published: November 2019

Research on materials with perovskite crystal symmetry for photonics applications represent a rapidly growing area of the photonics development due to their unique optical and electrical properties. Among them are high charge carrier mobility, high photoluminescence quantum yield, and high extinction coefficients, which can be tuned through all visible range by a controllable change in chemical composition. To date, most of such materials contain lead atoms, which is one of the obstacles for their large-scale implementation. This disadvantage can be overcome via the substitution of lead with less toxic chemical elements, such as Sn, Bi, Yb, etc., and their mixtures. Herein, we summarized the scientific works from 2016 related to the lead-free perovskite materials with stress on the lasing and lighting applications. The synthetic approaches, chemical composition, and morphology of materials, together with the optimal device configurations depending on the material parameters are summarized with a focus on future challenges.
Maize Straw as a Valuable Energetic Material for Biogas Plant Feeding
Authors: Mazurkiewicz Jakub , Marczuk Andrzej , Pochwatka Patrycja , Kujawa Sebastian
Published: November 2019

Maize has great potential, especially as a substrate for biofuels production. The aim of this paper is to analyze the possibility of usage in methane fermentation maize straw harvested in different weather conditions, which had an influence on different physical parameters, mainly the dry mass content. The research has shown that maize straw harvested in Central-Eastern Europe can have a broad spectrum of dry mass content, which is related to diverse weather conditions during autumn. However, independently from moisture content, maize straw can be a good (for more wet material) or very good (for more dried straw) substrate for the biogas plant. With the methane productivity reaching 201–207 m3/Mg of fresh mass, this material is a significantly better substrate than that typically used in Europe maize silage (approximately 105 m3/Mg FM). It was noted that the retention time for maize straw (36–42 days) is longer than in the case of maize silage (less than 30 days). However, this difference is quite small and can be accepted by the biogas plant operators.
On The Influence of Rotary Dresser Geometry on Wear Evolution and Grinding Process
Authors: Godino Leire , Alvarez Jorge , Muñoz Arkaitz , Pombo Iñigo
Published: November 2019

Dressing is a critical issue for optimizing the grinding process. Dresser tool and dresser parameters must be designed according to the grinding wheel material, shape, or even the dimensional and geometrical tolerances of the workpiece and its surface roughness. Likewise, one of the problematic issues of dressers is the wear that they suffer. In order to tackle this issue, the present work characterized the wear of two rotary dressers by analysing the wear behaviour depending on the pit radius of the dressers while studying the influence of the wear on ground surfaces. This work showed that the rotary dresser with a higher pit radius presents wear that is approximately 28% higher than the dresser with a half pit radius.
Preparation and Photocathodic Protection Properties of ZnO/TiO2 Heterojunction Film Under Simulated Solar Light
Authors: Zhang Xiong , Chen Guanghui , Li Weihua , Wu Dianwu
Published: November 2019

In this work, a novel double layer made of ZnO nanorod arrays and TiO2 nanorod arrays with anticorrosion function were successfully prepared on fluorine-doped tin oxide (FTO) substrate by a simple low-temperature solvothermal method. As compared with the pure TiO2 and pure ZnO film, the combination of the two films presented higher photocathodic protection performance for 316 stainless steel (316 SS) and Q235 carbon steel in 3.5 wt% NaCl solution. The composite film with ZnO nanoparticles layer between ZnO nanorod arrays and TiO2 nanorod arrays exhibited the best photocathodic performance, which lowered the open circuit potential (OCP) of 316 SS and Q235 to −991 mV, −1066 mV, respectively. The results demonstrated that the formation of the uniform heterojunction film and the small difference in band alignment played important roles in the promotion of photocathodic protection performance.
Silver-Copper Oxide Heteronanostructures for the Plasmonic-Enhanced Photocatalytic Oxidation of N-Hexane in the Visible-NIR Range
Authors: Suarez Hugo , Ramirez Adrian , Bueno-Alejo Carlos J. , Hueso Jose L.
Published: November 2019

Volatile organic compounds (VOCs) are recognized as hazardous contributors to air pollution, precursors of multiple secondary byproducts, troposphere aerosols, and recognized contributors to respiratory and cancer-related issues in highly populated areas. Moreover, VOCs present in indoor environments represent a challenging issue that need to be addressed due to its increasing presence in nowadays society. Catalytic oxidation by noble metals represents the most effective but costly solution. The use of photocatalytic oxidation has become one of the most explored alternatives given the green and sustainable advantages of using solar light or low-consumption light emitting devices. Herein, we have tried to address the shortcomings of the most studied photocatalytic systems based on titania (TiO2) with limited response in the UV-range or alternatively the high recombination rates detected in other transition metal-based oxide systems. We have developed a silver-copper oxide heteronanostructure able to combine the plasmonic-enhanced properties of Ag nanostructures with the visible-light driven photoresponse of CuO nanoarchitectures. The entangled Ag-CuO heteronanostructure exhibits a broad absorption towards the visible-near infrared (NIR) range and achieves total photo-oxidation of n-hexane under irradiation with different light-emitting diodes (LEDs) specific wavelengths at temperatures below 180 °C and outperforming its thermal catalytic response or its silver-free CuO illuminated counterpart.
Marine Polysaccharides in Pharmaceutical Applications: Fucoidan and Chitosan as Key Players in the Drug Delivery Match Field
Authors: Barbosa Ana Isabel , Coutinho Ana Joyce , Costa Lima Sofia A. , Reis Salette
Published: November 2019

The use of marine-origin polysaccharides has increased in recent research because they are abundant, cheap, biocompatible, and biodegradable. These features motivate their application in nanotechnology as drug delivery systems; in tissue engineering, cancer therapy, or wound dressing; in biosensors; and even water treatment. Given the physicochemical and bioactive properties of fucoidan and chitosan, a wide range of nanostructures has been developed with these polysaccharides per se and in combination. This review provides an outline of these marine polysaccharides, including their sources, chemical structure, biological properties, and nanomedicine applications; their combination as nanoparticles with descriptions of the most commonly used production methods; and their physicochemical and biological properties applied to the design of nanoparticles to deliver several classes of compounds. A final section gives a brief overview of some biomedical applications of fucoidan and chitosan for tissue engineering and wound healing.
Role of Jumpstart Nutrition®, a Dietary Supplement, to Ameliorate Calcium-to-Phosphorus Ratio and Parathyroid Hormone of Patients with Osteoarthritis
Authors: Ganguly Apurba
Published: November 2019

The aim of this study was to use Jumpstart Nutrition® bone supplementing combination with vitamin-K2 and coenzyme-Q10 characterized by an innovative delivery system that improves bioavailability of calcium-to-phosphorus ratio (CPR) and parathyroid hormone (PTH) in the management of osteoarthritis (OA). This eight-week registry included 108 patients treated for symptomatic OA confirmed with radiological images. On top of that, 63 patients used Jumpstart Nutrition® supplement, mainly prepared with calcium, phosphorus, coenzyme-Q10, vitamin-K2, vitamin-D2, vitamin-C, folic acid, curcumin and boswellic acids. Rescue medication was also recommended. Patients’ pain and functional capacity through outcome measures—knee-injury osteoarthritis outcome scale (KOOS) and Karnofsky performance scale (KPS), biomarkers such as levels of CPR, PTH and 25-hydroxy-vitamin-D were evaluated for the groups with and without supplement using appropriate kits. After eight weeks, the levels of CPR and PTH were all significantly improved (p < 0.001), fewer subjects had to use rescue medication (p < 0.05) and variation of pain and functional capacity under KOOS and KPS (p < 0.05) of the patients in the supplement group compared to controls. This registry study indicates that Jumpstart Nutrition® can be used safely for effective management of OA patients for the amelioration of CPR, PTH and functional activities confirmed with biomarkers and radiological images correlated with the Kellgren-Lawrance scale.