bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Online Behavioural Responses to Transcranial Direct Current Stimulation in Stroke Patients with Anomia
Authors: Hope Thomas M. H. , Ondobaka Sasha , Akkad Haya , Nardo Davide , Pappa Katerina , Price Cathy J. , Leff Alexander P. , Crinion Jennifer T.
Published: March 2024

Anomia, or difficulty naming common objects, is the most common, acquired impairment of language. Effective therapeutic interventions for anomia typically involve massed practice at high doses. This requires significant investment from patients and therapists. Aphasia researchers have increasingly looked to neurostimulation to accelerate these treatment effects, but the evidence behind this intervention is sparse and inconsistent. Here, we hypothesised that group-level neurostimulation effects might belie a more systematic structure at the individual level. We sought to test the hypothesis by attempting to predict the immediate (online), individual-level behavioural effects of anodal and sham neurostimulation in 36 chronic patients with anomia, performing naming and size judgement tasks. Using clinical, (pre-stimulation) behavioural and MRI data, as well as Partial Least Squares regression, we attempted to predict neurostimulation effects on accuracies and reaction times of both tasks. Model performance was assessed via cross-validation. Predictive performances were compared to that of a null model, which predicted the mean neurostimulation effects for all patients. Models derived from pre-stimulation data consistently outperformed the null model when predicting neurostimulation effects on both tasks’ performance. Notably, we could predict behavioural declines just as well as improvements. In conclusion, inter-patient variation in online responses to neurostimulation is, to some extent, systematic and predictable. Since declines in performance were just as predictable as improvements, the behavioural effects of neurostimulation in patients with anomia are unlikely to be driven by placebo effects. However, the online effect of the intervention appears to be as likely to interfere with task performance as to improve it.
Peripheral Artery Disease Ultrasound Assessment in Predicting the Severity of Coronary Artery Disease
Authors: Olinic Maria , Lazar Florin-Leontin , Onea Horea-Laurentiu , Homorodean Calin , Ober Mihai , Tataru Dan , Spinu Mihail , Achim Alexandru , Olinic Dan-Mircea
Published: March 2024

Atherosclerosis in a progressive disease that is systemic in nature, and hence the simultaneous presentation of coronary artery disease (CAD) and peripheral artery disease (PAD) is not uncommon. As clinically manifested PAD is associated with worse cardiovascular outcomes, the timely identification of subclinical atherosclerosis seems of utmost importance. Ultrasonography (US) is an ideal imaging modality for assessing PAD that is easy to use, accurate, widely available and avoids unnecessary exposure to radiation. Several US parameters have been proposed in the assessment of PAD, with varying prognostic usefulness, depending on disease location. The aim of this review is to summarize the most important evidence available on the association between US-detected atherosclerosis in different vascular sites and the presence and severity of CAD, as well as the impact of the early detection of PAD on the outcomes of patients presenting with CAD.
Diagnosis of the Condition of Aquatic Ecosystems Using a Partial Assessment of Ecological and Trophic States: An Example of Small Lakes in Northern Poland
Authors: Markowski Maciej , Wojtasik Barbara
Published: March 2024

The current state of aquatic ecosystems was assessed for lakes in two river–lake systems: Święta Struga (Święte and Ocypelek) and Kałębnica (Czarne Południowe and Kałębie). Trophic states were determined using the Vollenweider, Nürnberg, and Carlson methods. Additionally, shoreline changes were analyzed for all lakes concerning their natural character, and physicochemical parameters were utilized as auxiliary elements in the assessment of the ecological state following the guidelines of Directive 2000/60/EC. Supplementary to these methods, the ecological evaluation of the littoral zone utilized meiobenthic organism grouping as an indicator. The results indicate significant differences among the studied reservoirs. Lake Święte is a reservoir bordering mesotrophy and eutrophy, with the ecological state of the littoral zone showing ongoing trophic development. Lake Ocypelek exemplifies a eutrophic reservoir undergoing progressive degradation. The lakes in the Kałębnica system also exhibit advanced trophic conditions, with Lake Kałębie being a eutrophic reservoir. Its shoreline has been significantly altered due to the development of the direct catchment area. In contrast, Lake Czarne Południowe serves as an example of a mesotrophic reservoir, with a substantially changed shoreline. Considering physicochemical elements and chlorophyll a concentration, the water quality for all examined lakes was found to be below the good category. The diagnosis of the condition of lake aquatic ecosystems requires an individualized approach to each of them. Therefore, employing various methods for their assessment, encompassing physico-chemical, biological, and morphological parameters, provides the opportunity to initiate potential remedial actions that will effectively contribute to their improvement. Conducting low-cost research involving a multidisciplinary approach to diagnose the state of aquatic ecosystems is becoming a crucial element in lake monitoring.
Comparison of Oncological Outcomes between Transperitoneal and Retroperitoneal Approaches in Laparoscopic Nephroureterectomies for Upper Tract Urothelial Carcinoma
Authors: Otoshi Taiyo , Yamasaki Takeshi , Matsue Taisuke , Yukimatsu Nao , Kato Minoru , Machida Yuichi , Iwai Tomoaki , Kuratsukuri Katsuyuki , Uchida Junji
Published: March 2024

Background and Objectives: Our aim was to clarify the oncological outcomes of the two different approaches to laparoscopic nephroureterectomies (LNUs) in Japan, and to examine whether there were any significant differences between the transperitoneal approach and the retroperitoneal approach. Materials and Methods: We retrospectively evaluated patients who underwent an LNU for upper tract urothelial carcinoma (UTUC) from January 2013 to December 2022. We identified 52 patients who underwent a transperitoneal LNU (tLNU) and 93 who underwent a retroperitoneal LNU (rLNU). We adopted age, smoking, and pT-stage matching, and 43 patients were classified in each group. We investigated the time from surgery to recurrence (RFS: recurrence-free survival), the time to death (OS: overall survival), and the time to non-urothelial-tract recurrence-free survival (NUTRFS). A Cox regression analysis was performed to evaluate the risk factors that influenced recurrence. Results: There were no significant differences in the RFS, OS, and NUTRFS between the two matched groups. In the multivariate Cox regression analysis, the pT stage (pT3≥ vs. pT2≤) had an HR = 2.09 and a p = 0.01, and was an independent prognostic risk factor regarding cancer recurrence. Conclusions: There were no significant differences in the oncological outcomes between the tLNU and rLNU groups. It is suggested that the transperitoneal approach should be selected for LNUs.
Be the Change You Want to See: Problem-Based Learning to Promote Diversity, Justice, Equity, Inclusion, Belonging, and Sustainability in the Classroom and Workplace
Authors: Renz Franziska M. , Vogel Julian U. N.
Published: March 2024

This study presents a problem-based learning (i.e., PBL) assignment to engage students around the areas of diversity, justice, equity, inclusion, belonging (i.e., DEI), and sustainability in the classroom and workplace. The assignment is developed based on the learning objectives of an upper division business course following an outlined three-step process. A pretest-posttest research design with a control group demonstrates that the experiential assignment is effective in enhancing students’ problem-solving skills which, according to employers, recent college graduates need to improve upon. The PBL approach also increases students’ interest in DEI and sustainability so that they want to make a difference in society. While students’ confidence in their preparedness to become a manager decreases over the course of a semester, this loss in confidence is mitigated by students’ active participation in the PBL assignment.
Residual Stresses and the Microstructure of Modeled Laser-Hardened Railway Axle Seats under Fatigue
Authors: Čapek Jiří , Trojan Karel , Kec Jan , Ganev Nikolaj , Černý Ivo , Mužík Tomáš
Published: March 2024

Railway wheels are usually attached to axles by press-fitting; therefore, the mechanical processes taking place during operation can result in failure, with fatal consequences for the axle seats. This manuscript describes the effect of laser hardening on the residual stress state, microstructural parameters (lattice defects—dislocations, crystallites, microstrains, etc.), and mechanical properties of laser-hardened EA1N steel railway axles under fatigue life conditions. Differences were found between ground, single-track, and multi-track hardened surfaces. Tensile residual stresses, low dislocation densities and hardnesses, and different microstructures (tempered cubic martensite) were found at the overlapped tracks and at the boundary of the heat-affected zone and bulk surface compared with the hardened zone. As a result, the surface treatment of axle seats by laser hardening improved the fatigue failure resistance compared with untreated seats. Optimal properties of the integrity of the axle seat surface were achieved, including fatigue resistance, which seems to be positively influenced mainly by sufficient hardness and the appropriate microstructure. The influence of the other investigated parameters was not evident, and was reduced by the presence of fretting corrosion and press-fitting.
Optimisation of the Heat Treatment Profile for Powder-Bed Fusion Built AlSi10Mg by Age Hardening and Ice-Water Quenching
Authors: Mfusi Busisiwe J. , Popoola Patricia Abimbola , Mathe Ntombizodwa R.
Published: March 2024

During powder-bed fusion (PBF), the irradiated material causes undesirable thermal stresses while experiencing large temperature oscillations over a rapid period. This requires the components produced by this technique to undergo thermal treatment. The characteristics of additively manufactured materials, which are rapid heating and cooling, do not accept conventional methods, such as thermal treatment, that alleviate stress for the removal of thermal stresses. In this research, the thermal treatment of age hardening is explored, in which AlSi10Mg is subjected to lower temperatures for longer periods of time. Other samples were thermally treated at 300 °C and 400 °C for various hours and quenched in ice water. This is conducted to identify the acceptable temperature and conditions that will improve the properties after thermal treatment without jeopardising other properties of the material and to investigate the effects of the thermal treatment profiles on the microstructural and mechanical characteristics of the AlSi10Mg samples.
Low-Temperature Impact Fracture Behavior of Medium Manganese Steel with Bcc-fcc Duplex Microstructures
Authors: Du Yu , Gao Xiuhua , Wang Xiaonan , Wu Hongyan , Sun Chao , Sun Guosheng , Du Linxiu
Published: March 2024

Impact fracture behavior at low temperatures was investigated in medium manganese steel with bcc-fcc duplex microstructures. The impact energy was above 150 J (−80~20 °C) and the fractography showed dimples for inter-critical annealing at 630 °C (QHA) because of the high retained austenite stability and low martensite dislocation density. The impact energy was from 180 J (20 °C) to 60 J (−80 °C) and the fractography was intergranular for inter-critical annealing at 610 °C (QLA) because of the low stability of RA and carbides precipitated at the prior austenite grain boundaries. The impact energy was below 60 J (−80~20 °C) and the fractography showed cleavage for direct quenching (DQ) because of the high dislocation density of martensite.
Applied Magnetic Field Increases Magnetic Anisotropy in HDDR-Processed Nd-Fe-B Alloy
Authors: Tener Zachary P. , Liu Xubo , Nlebedim Ikenna C. , Kramer Matthew J. , McGuire Michael A. , Kesler Michael S.
Published: March 2024

We investigate the effect of an applied magnetic field on the entire HDDR process using a customized reactor vessel and a warm-bore superconducting magnet. We analyzed the resulting properties produced at both a 0 applied field and a 2 Tesla applied field. We show that the application of a magnetic field throughout the HDDR process results in powders that exhibit a greater level of anisotropy compared to their ambient field counterparts.
A Load-Adaptive Driving Method for a Quasi-Continuous-Wave Laser Diode
Authors: Wu Yajun , Liu Wenqing , Sun Xinhui , Chen Jinxin , Cheng Gang , Chen Xi , Fu Yibin , Liu Pan , Zhang Tianshu
Published: March 2024

A quasi-continuous-wave (QCW) laser diode (LD) driver is commonly used to drive diode bars and stacks designed specifically for QCW operations in solid-state lasers. Such drivers are optimized to deliver peak current and voltage pulses to LDs while maintaining low average power levels. As a result, they are widely used in laser processing devices and laser instruments. Traditional high-energy QCW LD drivers primarily use capacitors as energy storage components and pulsed constant-current sources with op-amps and power metal-oxide-semiconductor field-effect transistors (MOSFETs) as their core circuits for generating repeated constant-current pulses. The drawback of this type of driver is that the driver’s output voltage needs to be manually adjusted according to the operating voltage of the load before use to maximize driver efficiency while providing a sufficient current. Another drawback is its inability to automatically adjust the output voltage to maintain high efficiency when the load changes during the driver operation. Drastic changes in the load can cause the driver to fail to function properly in extreme cases. Based on the above traditional circuit structure, this study designed a stability compensation circuit and realized a QCW LD driver for driving a GS20 diode stack with a maximum repetition rate of 100 Hz, a constant current of approximately 300 A, a load voltage of approximately 10 V, and a pulse width of approximately 300 μs. In particular, a high-efficiency, load-adaptive driving method was used with the MOSFETs in the critical saturation region (i.e., between the linear and saturated regions), controlling its power loss effectively while achieving maximum output current of the driver. The experiments demonstrated that the driver efficiency could be maintained at more than 80% when the load current varied from 50 to 300 A.
Broadband Balanced-to-Balanced Filtering Power Divider Using HMSIW-SSPP Transmission Line
Authors: Liu Hao , Xue Bing , Xu Jun
Published: March 2024

In this paper, a novel broadband balanced-to-balanced (BTB) filtering power divider (FPD) utilizing the half-mode substrate-integrated waveguide and spoof surface plasmon polariton (HMSIW-SSPP) hybrid transmission line is introduced. Initially, a new HMSIW-SSPP unit cell is proposed, demonstrating a lower upper cut-off frequency compared to the classical HMSIW-SSPP unit cell. Building upon this unit cell, a bandpass BTB FPD is devised employing dual-layer stacked substrates, enabling independent control over the passband’s lower and upper cut-off frequencies through specific physical dimensions. Additionally, the incorporation of isolation resistors and defected ground structures in the BTB FPD enhances differential-mode isolation and common-mode (CM) suppression between output ports. A manufactured and tested BTB FPD prototype validates this design method, showcasing a broad fractional bandwidth of 52.31% (6.72–11.48 GHz), output port isolation surpassing 14.25 dB, and transmitted CM suppression exceeding 34.05 dB.
Material Design of Ultra-Thin InN/GaN Superlattices for a Long-Wavelength Light Emission
Authors: Xiang Leilei , Zhang Enming , Kang Wenyu , Lin Wei , Kang Junyong
Published: March 2024

GaN heterostructure is a promising material for next-generation optoelectronic devices, and Indium gallium nitride (InGaN) has been widely used in ultraviolet and blue light emission. However, its applied potential for longer wavelengths still requires exploration. In this work, the ultra-thin InN/GaN superlattices (SL) were designed for long-wavelength light emission and investigated by first-principles simulations. The crystallographic and electronic properties of SL were comprehensively studied, especially the strain state of InN well layers in SL. Different strain states of InN layers were applied to modulate the bandgap of the SL, and the designed InN/GaN heterostructure could theoretically achieve photon emission of at least 650 nm. Additionally, we found the SL had different quantum confinement effects on electrons and holes, but an efficient capture of electron-hole pairs could be realized. Meanwhile, external forces were also considered. The orbital compositions of the valence band maximum (VBM) were changed with the increase in tensile stress. The transverse electric (TE) mode was found to play a leading role in light emission in normal working conditions, and it was advantageous for light extraction. The capacity of ultra-thin InN/GaN SL on long-wavelength light emission was theoretically investigated.
Behavior of Tantalum in a Fe-Dominated Synthetic Fayalitic Slag System—Phase Analysis and Incorporation
Authors: Schirmer Thomas , Hiller Jessica , Weiss Joao , Munchen Daniel , Lucas Hugo , Fittschen Ursula E. A. , Friedrich Bernd
Published: March 2024

Pyrometallurgical processes produce slags that may contain valuable elements because of their high oxygen affinity. However, the concentration is extremely low, which causes losses. In fact, these elements, for example, tantalum and rare earth elements, are less than 1% recycled. To return such technologically important elements to the material cycle, pyrometallurgically is used to enrich them in the simplest possible compounds within the slag, which have favorable properties for recovery (morphology, crystal size, magnetic properties), allowing further mechanical separation. The purpose of modification of the slag system is to obtain engineered artificial minerals” (EnAM), a process in which targeted minerals with high element concentration are formed. In this article, this approach is investigated using tantalum-rich fayalitic slag, since this slag is commonly found in the industry for the pyrometallurgical treatment of waste electric and electronic equipment. Synthetic fayalitic slags in reducing environment under different cooling rates were produced with Ta addition. The characterization of the produced samples was carried out using powder X-ray diffraction (PXRD) and electron probe microanalysis (EPMA). Additionally, the speciation of Fe and Ta was accessible through X-ray absorption near-edge structure (XANES) spectroscopy. EPMA also provided a semiquantitative assessment of the Ta distribution in these individual compounds. In these slags, tantalum accumulated in perovskite-like oxidic and silicate compounds as well as in magnetic iron oxides. The enrichment factor is highest in tantalite/perovskite-type oxides (FexTayO6, CaxFeyTazO3) with up to 60 wt.% Ta and ‘tantalomagnetite’ (FeII(FeIII(2-5/3x)Tax)O4) with a maximum of ~30 wt.% Ta (only fast cooling). This is followed by a perovskite-like silicon containing oxide (XYO3) with 12–15 wt.% Ta (only slow cooling), and a hedenbergite-like compound (XYZ2O6) with a varying content of 0.3–7 wt.%. The Ta concentration in pure Fe, Fe(1-x)O, hercynitic spinel and hematite is negligible. Despite the very low phase fraction, the most promising EnAM compound is nevertheless perovskite-like tantalum oxide, as the highest enrichment factor was obtained. Tantalum-rich magnetite-like oxides also could be promising.
Application of Minerals for the Characterization of Geothermal Reservoirs and Cap Rock in Intracontinental Extensional Basins and Volcanic Islands in the Context of Subduction
Authors: Ledésert Béatrice A.
Published: March 2024

Whether from the near-surface or at great depths, geothermal energy aims to harness the heat of the Earth to produce energy. Herein, emphasis is put on geothermal reservoirs and their cap rock in crystalline rocks, in particular, the basements of sedimentary basins and volcanic islands in the context of subduction. This study is based on a case study of three examples from around the world. The aim of this paper is to show how the study of newly formed minerals can help the exploration of geothermal reservoirs. The key parameters to define are the temperature (maximum temperature reached formerly), fluid pathways, and the duration of geothermal events. To define these parameters, numerous methods are used, including optical and electronic microscopy, X-ray diffraction, microthermometry on fluid inclusions, chlorite geothermometry, and geochemistry analysis, including that of isotopes. The key minerals that are studied herein are phyllosilicates and, in particular, clay minerals, quartz, and carbonates. They are formed because of hydrothermal alterations in fracture networks. These minerals can have temperatures of up to 300 °C (and they can cool down to 50 °C), and sometimes, they allow for one to estimate the cooling rate (e.g., 150 °C/200 ka). The duration of a hydrothermal event (e.g., at least 63 Ma or 650 ka, depending on the site) can also be established based on phyllosilicates.
Two-Dimensional MXene as a Promising Adsorbent for Trihalomethanes Removal: A Density-Functional Theory Study
Authors: Gomaa Islam , Hosny Nasser Mohammed , Elhaes Hanan , Ezzat Hend A. , Elmahgary Maryam G. , Ibrahim Medhat A.
Published: March 2024

This groundbreaking research delves into the intricate molecular interactions between MXene and trihalomethanes (THs) through a comprehensive theoretical study employing density-functional theory (DFT). Trihalomethanes are common carcinogenic chlorination byproducts found in water sanitation systems. This study focuses on a pristine MXene [Mn+1·Xn] monolayer and its various terminal [Tx] functional groups [Mn+1·XnTx], strategically placed on the surface for enhanced performance. Our investigation involves a detailed analysis of the adsorption energies of THs on different MXene types, with the MXene-Cl layer emerging as the most compatible variant. This specific MXene-Cl layer exhibits remarkable properties, including a total dipole moment (TDM) of 12.443 Debye and a bandgap of 0.570 eV, achieved through meticulous geometry optimization and computational techniques. Notably, THs such as trichloromethane (CHCl3), bromide-chloromethane (CHBrCl2), and dibromochloromethane (CHBr2Cl) demonstrate the highest TDM values, indicating substantial changes in electronic and optical parameters, with TDM values of 16.363, 15.998, and 16.017 Debye, respectively. These findings highlight the potential of the MXene-Cl layer as an effective adsorbent and detector for CHF3, CHClF2, CHCl3, CHBrCl2, and CHBr2Cl. Additionally, we observe a proportional increase in the TDM and bandgap energy, indicative of conductivity, for various termination atom combinations, such as Mxene-O-OH, Mxene-O-F, Mxene-O-Cl, Mxene-OH-F, Mxene-F-Cl, and Mxene-OH-Cl, with bandgap energies measured at 0.734, 0.940, 1.120, 0.835, and 0.927 eV, respectively. Utilizing DFT, we elucidate the adsorption energies of THs on different MXene surfaces. Our results conclusively demonstrate the significant influence of the termination atom nature and quantity on MXene’s primitive TDM value. This research contributes to our understanding of MXene–THs interactions, offering promising avenues for the development of efficient adsorbents and detectors for THs. Ultimately, these advancements hold the potential to revolutionize water sanitation practices and enhance environmental safety.
Correction: Ji et al. Mesoporous Cobalt Oxide (CoOx) Nanowires with Different Aspect Ratios for High Performance Hybrid Supercapacitors. Nanomaterials 2023, 13, 749
Authors: Ji Haomin , Ma Yifei , Cai Zhuo , Yun Micun , Han Jiemin , Tong Zhaomin , Wang Mei , Suhr Jonghwan , Xiao Liantuan , Jia Suotang , Chen Xuyuan
Published: March 2024

There was an error in the original publication [...]
Visuo-Attentional and Phonological Deficits Explored in French Students with Dyslexia: Eye Movements Recorded during a Phonological Lexical Decision Task
Authors: Premeti Aikaterini , Isel Frédéric , Bucci Maria Pia
Published: March 2024

Whether dyslexia is caused by phonological or attentional dysfunction remains a widely debated issue. To enrich this debate, we compared the eye movements of 32 French university students with (14 students) and without (18 students) dyslexia while performing a delayed phonological lexical decision task on 300 visually presented stimuli. The processing stimuli involved either a lexical (i.e., words) or a non-lexical route relying on a grapheme-phoneme correspondence (pseudohomophones and pseudowords), while other stimuli involved only a visual search (consonant and symbol sequences). We recorded the number of fixations, the duration of the first fixation and the amplitude of saccades made on the stimuli. Compared to the controls, the participants with dyslexia made more fixations while reading regardless of the type of stimulus (lexical and non-lexical). Crucially, the participants with dyslexia exhibited longer first fixations in particular while reading phonologically challenging stimuli such as pseudohomophones and pseudowords compared to stimuli involving a simple visual search (consonants, symbols). Taken together, these results suggest that both visual and phonological impairments may be implicated in dyslexia, supporting the hypothesis that dyslexia is a multifactorial deficit.
Evaluation of Antiproliferative Properties of CoMnZn-Fe2O4 Ferrite Nanoparticles in Colorectal Cancer Cells
Authors: Narayanaswamy Venkatesha , Rah Bilal , Al-Omari Imaddin A. , Kamzin Alexander S. , Khurshid Hafsa , Muhammad Jibran Sualeh , Obaidat Ihab M. , Issa Bashar
Published: March 2024

The PEG-coated ferrite nanoparticles Co0.2Mn0.6Zn0.2Fe2O4 (X1), Co0.4Mn0.4Zn0.2Fe2O4 (X2), and Co0.6Mn0.2Zn0.2Fe2O4 (X3) were synthesized by the coprecipitation method. The nanoparticles were characterized by XRD, Raman, VSM, XPS, and TEM. The magnetic hyperthermia efficiency (MH) was determined for PEG-coated nanoparticles using an alternating magnetic field (AMF). X2 nanoparticles displayed the highest saturation magnetization and specific absorption rate (SAR) value of 245.2 W/g for 2 mg/mL in a water medium. Based on these properties, X2 nanoparticles were further evaluated for antiproliferative activity against HCT116 cells at an AMF of 495.25 kHz frequency and 350 G strength, using MTT, colony formation, wound healing assays, and flow cytometry analysis for determining the cell viability, clonogenic property, cell migration ability, and cell death of HCT116 cells upon AMF treatment in HCT116 cells, respectively. We observed a significant inhibition of cell viability (2% for untreated control vs. 50% for AMF), colony-forming ability (530 cells/colony for untreated control vs. 220 cells/colony for AMF), abrogation of cell migration (100% wound closure for untreated control vs. 5% wound closure for AMF), and induction of apoptosis-mediated cell death (7.5% for untreated control vs. 24.7% for AMF) of HCT116 cells with respect to untreated control cells after AMF treatment. Collectively, these results demonstrated that the PEG-coated (CoMnZn-Fe2O4) mixed ferrite nanoparticles upon treatment with AMF induced a significant antiproliferative effect on HCT116 cells compared with the untreated cells, indicating the promising antiproliferative potential of the Co0.4Mn0.4Zn0.2Fe2O4 nanoparticles for targeting colorectal cancer cells. Additionally, these results provide appealing evidence that ferrite-based nanoparticles using MH could act as potential anticancer agents and need further evaluation in preclinical models in future studies against colorectal and other cancers.
The Formulation and Characterization of Wound Dressing Releasing S-Nitrosoglutathione from Polyvinyl Alcohol/Borax Reinforced Carboxymethyl Chitosan Self-Healing Hydrogel
Authors: Palungan Juliana , Luthfiyah Widya , Mustopa Apon Zaenal , Nurfatwa Maritsa , Rahman Latifah , Yulianty Risfah , Wathoni Nasrul , Yoo Jin-Wook , Hasan Nurhasni
Published: March 2024

Self-healing hydrogels often lack mechanical properties, limiting their wound-dressing applications. This study introduced S-Nitrosoglutathione (GSNO) to self-healing hydrogel-based wound dressings. Self-healing hydrogel mechanical properties were improved via polymer blends. Applying this hydrogel to the wound site allows it to self-heal and reattach after mechanical damage. This work evaluated polyvinyl alcohol (PVA)-based self-healing hydrogels with borax as a crosslinking agent and carboxymethyl chitosan as a mechanical property enhancer. Three formulations (F1, F4, and F7) developed self-healing hydrogels. These formulations had borax concentrations of 0.8%, 1.2%, and 1.6%. An FTIR study shows that borate ester crosslinking and hydrogen bonding between polymers generate a self-healing hydrogel. F4 has a highly uniform and regular pore structure, as shown by the scanning electron microscope image. F1 exhibited faster self-healing, taking 13.95 ± 1.45 min compared to other formulations. All preparations had pH values close to neutrality, making them suitable wound dressings. Formula F7 has a high drug content (97.34 ± 1.21%). Good mechanical qualities included high tensile stress–strain intensity and Young’s modulus. After 28 h of storage at −20 °C, 5 °C, and 25 °C, the self-healing hydrogel’s drug content dropped significantly. The Korsmeyer–Peppas release model showed that the release profile of GSNO followed Fickian diffusion. Thus, varying the concentration of crosslinking agent and adding a polymer affects self-healing hydrogels’ physicochemical properties.
Thankfulness: Kierkegaard’s First-Person Approach to the Problem of Evil
Authors: Schulz Heiko
Published: March 2024

The present paper argues that, despite appearance to the contrary, Kierkegaard’s writings offer promising argumentational resources for addressing the problem of evil. According to Kierkegaard, however, in order to make use of these resources at all, one must necessarily be willing to shift the battleground, so to speak: from a third- to a genuine first-person perspective, namely the perspective of what Climacus dubs Religiousness A. All (yet also only) those who seek deliberate self-annihilation before God—a God in relation to whom they perceive themselves always in the wrong—shall discover the ideal that an unwavering and in fact unconditional thankfulness (namely, for being forgiven) is to be considered the only appropriate attitude towards God and as such both necessary and sufficient for coming to terms with evil and suffering, at least in the life of someone making that discovery. I will argue that Kierkegaard’s (non-)pseudonymous writings provide reasons, at times unwittingly, for adopting the perspective of Religiousness A; however, I will also and ultimately argue that the principle of infinite thankfulness as a corollary of that perspective flounders when it comes to making sense of (the eschatological implications of) the suffering of others.
Holistic Approaches to Plant Stress Alleviation: A Comprehensive Review of the Role of Organic Compounds and Beneficial Bacteria in Promoting Growth and Health
Authors: Hathurusinghe Sandamali Harshani Kumari , Azizoglu Ugur , Shin Jae-Ho
Published: March 2024

Plants select microorganisms from the surrounding bulk soil, which act as a reservoir of microbial diversity and enrich a rhizosphere microbiome that helps in growth and stress alleviation. Plants use organic compounds that are released through root exudates to shape the rhizosphere microbiome. These organic compounds are of various spectrums and technically gear the interplay between plants and the microbial world. Although plants naturally produce organic compounds that influence the microbial world, numerous efforts have been made to boost the efficiency of the microbiome through the addition of organic compounds. Despite further crucial investigations, synergistic effects from organic compounds and beneficial bacteria combinations have been reported. In this review, we examine the relationship between organic compounds and beneficial bacteria in determining plant growth and biotic and abiotic stress alleviation. We investigate the molecular mechanism and biochemical responses of bacteria to organic compounds, and we discuss the plant growth modifications and stress alleviation done with the help of beneficial bacteria. We then exhibit the synergistic effects of both components to highlight future research directions to dwell on how microbial engineering and metagenomic approaches could be utilized to enhance the use of beneficial microbes and organic compounds.
Ontogenetic Changes in the Feeding Behaviour of Helicoverpa armigera Larvae on Pigeonpea (Cajanus cajan) Flowers and Pods
Authors: Volp Trevor M. , Zalucki Myron P. , Furlong Michael J.
Published: March 2024

Despite substantial research examining caterpillar–plant interactions, changes in the feeding behaviour of lepidopteran larvae as they develop are poorly understood. In this study, we investigated ontogenetic changes in the behaviour of Helicoverpa armigera larvae feeding on reproductive structures of pigeonpea (Cajanus cajan). Specifically, we examined the preference for and avoidance of pigeonpea flowers and pods of first, second, third, and fourth instar H. armigera larvae. We also conducted a no-choice assay to compare the ability of third and fourth instar larvae to penetrate pigeonpea pod walls, which act as a physical defence against herbivory. When presented with a choice between pigeonpea pods and flowers, different instars behaved differently. First and second instar larvae largely avoided pigeonpea pods, instead feeding on flowers; third instar larvae initially avoided pods, but by 24 h, did not strongly discriminate between the structures; and fourth instars demonstrated a preference for pods. When initially placed on pods, first instars were slower than other instars to leave these structures, despite pods being suboptimal feeding sites for small caterpillars. We identified a clear instar-specific ability to penetrate through the pod wall to reach the seeds. Most third instar larvae were unable to penetrate the pod wall, whereas most fourth instars succeeded. Third instars suffered a physiological cost (measured by relative growth rate) when boring through the pod wall, which was not observed in fourth instars. Our study further illuminates the insect–plant interactions of the H. armigera–pigeonpea system and provides evidence for the significant changes in feeding behaviour that may occur during lepidopteran larval development.
Effects of Rainfall Exclusion Treatment on Photosynthetic Characteristics of Black Locust in the Sub-Humid Region of the Loess Plateau, China
Authors: Guo Haining , Wang Yiran , Li Guoqing , Du Sheng
Published: March 2024

The mesic-origin species Robinia pseudoacacia L. (black locust) is widely planted in the semiarid and sub-humid areas of the Loess Plateau for the reforestation of vegetation-degraded land. Under the scenario of changing precipitation patterns, exploring the response of photosynthesis to drought allows us to assess the risk to sustainable development of these plantations. In this study, paired plots were established including the control and a treatment of 30% exclusion of throughfall (since 2018). The photosynthetic characteristics were investigated using a portable photosynthesis system for four periods in the full-leaf growing season of 2021–2022, the fourth and fifth years, on both treated and controlled sampling trees. Leaf gas exchange parameters derived from diurnal changing patterns, light response curves, and CO2 response curves showed significant differences except for period II (9–11 September 2021) between the two plots. The photosynthetic midday depression was observed in 2022 in the treated plot. Meanwhile, the decline of net photosynthetic rate in the treated plot was converted from stomatal limitation to non-stomatal limitation. Furthermore, we observed that black locust adapted to long-term water deficiency by reducing stomatal conductance, increasing water use efficiency and intrinsic water use efficiency. The results demonstrate that reduction in precipitation would cause photosynthesis decrease, weaken the response sensitivity to light and CO2, and potentially impair photosynthetic resilience of the plantations. They also provide insights into the changes in photosynthetic functions under global climate change and a reference for management of plantations.
Light Quality Effect on Internal N Retranslocation in Podocarpus macrophyllus Precultured with Exponential Nutrient Loading
Authors: Wang Yige , Sun Xiangyang , Li Suyan
Published: March 2024

Streetlamp light is inevitable in the night landscape of a city and may affect the phenology of newly planted ornamental plants, but it has rarely been fully examined. Newly transplanted ornamental plants probably suffer periodic shocks, which mainly result from the inefficient reuse of internal nutrients for new growth. Exponential nutrient loading (ENL) is well known for its ability to overcome transplant shocks by promoting retranslocation for the reuse of strengthened nutrients from internal reserves in precultured seedlings. Transplantation to urbanized lands is distinct from that of montane areas; this is mainly due to a high frequency of exposure to the artificial illumination of night lighting. It is suspected that this lighting modifies vegetative phenology and generates potential risks by increasing reliance on internal nutrient retranslocation. In this study, Podocarpus macrophyllus seedlings were cultured with ENL at low and high rates of nitrogen (N) deliveries (40 and 120 mg N seedling−1, respectively), and the high-rate treatment was identified as being able to trap seedlings within toxic states. A labeled 15N isotope was pulsed to transplanted seedlings exposed to simulated light qualities in red, green, and blue light spectra. The seedlings harvested at one month showed rare responses to the interactive spectra and preculture treatments, but most of them responded to the low-rate N preculture treatment with stronger abilities in terms of the reuse of internal N and the synthesizing of photosynthetic pigments. In conclusion, it was verified that night light enforces the effect on newly transplanted plants; the red light invoked internal N for reuse, and the blue light promoted the uptake of the current N. The internal N reserve established through preculture ENL rarely made a contribution to the night light effect, except for the enhancement of height growth in the red light. The red light spectrum was recommended for the exposure of newly transplanted seedlings due to its effect on the enhancement of the retranslocation of internal N and the induction of a steady state of uptake from the current N input.
Comparative Analysis of Sucrose-Regulatory Genes in High- and Low-Sucrose Sister Clones of Sugarcane
Authors: Khan Qaisar , Qin Ying , Guo Dao-Jun , Huang Yu-Yan , Yang Li-Tao , Liang Qiang , Song Xiu-Peng , Xing Yong-Xiu , Li Yang-Rui
Published: March 2024

Sugarcane is a significant primitive source of sugar and energy worldwide. The progress in enhancing the sugar content in sugarcane cultivars remains limited due to an insufficient understanding of specific genes related to sucrose production. The present investigation examined the enzyme activities, levels of reducing and non-reducing sugars, and transcript expression using RT-qPCR to assess the gene expression associated with sucrose metabolism in a high-sucrose sugarcane clone (GXB9) in comparison to a low-sucrose sister clone (B9). Sucrose phosphate synthase (SPS), sucrose phosphate phosphatase (SPP), sucrose synthase (SuSy), cell wall invertase (CWI), soluble acid invertase (SAI), and neutral invertase (NI) are essential enzymes involved in sucrose metabolism in sugarcane. The activities of these enzymes were comparatively quantified and analyzed in immature and maturing internodes of the high- and low-sucrose clones. The results showed that the higher-sucrose-accumulating clone had greater sucrose concentrations than the low-sucrose-accumulating clone; however, maturing internodes had higher sucrose levels than immature internodes in both clones. Hexose concentrations were higher in immature internodes than in maturing internodes for both clones. The SPS and SPP enzymes activities were higher in the high-sucrose-storing clone than in the low-sucrose clone. SuSy activity was higher in the low-sucrose clone than in the high-sucrose clone; further, the degree of SuSy activity was higher in immature internodes than in maturing internodes for both clones. The SPS gene expression was considerably higher in mature internodes of the high-sucrose clones than the low-sucrose clone. Conversely, the SuSy gene exhibited up-regulated expression in the low-sucrose clone. The enhanced expression of SPS in the high-sucrose clone compared to the low-sucrose clone suggests that SPS plays a major role in the increased accumulation of sucrose. These findings provide the opportunity to improve sugarcane cultivars by regulating the activity of genes related to sucrose metabolism using transgenic techniques.
MADS-Box Family Genes in Lagerstroemia indica and Their Involvement in Flower Development
Authors: Qiao Zhongquan , Deng Fuyuan , Zeng Huijie , Li Xuelu , Lu Liushu , Lei Yuxing , Li Lu , Chen Yi , Chen Jianjun
Published: March 2024

MADS-box is a key transcription factor regulating the transition to flowering and flower development. Lagerstroemia indica ‘Xiang Yun’ is a new cultivar of crape myrtle characterized by its non-fruiting nature. To study the molecular mechanism underlying the non-fruiting characteristics of ‘Xiang Yun’, 82 MADS-box genes were identified from the genome of L. indica. The physicochemical properties of these genes were examined using bioinformatics methods, and their expression as well as endogenous hormone levels at various stages of flower development were analyzed. The results showed that LiMADS genes were primarily classified into two types: type I and type II, with the majority being type II that contained an abundance of cis-acting elements in their promoters. By screening nine core proteins by predicted protein interactions and performing qRT-PCR analysis as well as in combination with transcriptome data, we found that the expression levels of most MADS genes involved in flower development were significantly lower in ‘Xiang Yun’ than in the wild type ‘Hong Ye’. Hormonal analysis indicated that ‘Xiang Yun’ had higher levels of iP, IPR, TZR, and zeatin during its early stages of flower development than ‘Hong Ye’, whereas the MeJA content was substantially lower at the late stage of flower development of ‘Hong Ye’. Finally, correlation analysis showed that JA, IAA, SA, and TZR were positively correlated with the expression levels of most type II genes. Based on these analyses, a working model for the non-fruiting ‘Xiang Yun’ was proposed. During the course of flower development, plant hormone response pathways may affect the expression of MADS genes, resulting in their low expression in flower development, which led to the abnormal development of the stamen and embryo sac and ultimately affected the fruiting process of ‘Xiang Yun’.
Effect of Functionalization of Texturized Polypropylene Surface by Silanization and HBII-RGD Attachment on Response of Primary Abdominal and Vaginal Fibroblasts
Authors: Quiles Maria Teresa , Rodríguez-Contreras Alejandra , Guillem-Marti Jordi , Punset Miquel , Sánchez-Soto Miguel , López-Cano Manuel , Sabadell Jordi , Velasco Janice , Armengol Manuel , Manero Jose Maria , Arbós Maria Antònia
Published: March 2024

Soft tissue defects, such as incisional hernia or pelvic organ prolapse, are prevalent pathologies characterized by a tissue microenvironment rich in fragile and dysfunctional fibroblasts. Precision medicine could improve their surgical repair, currently based on polymeric materials. Nonetheless, biomaterial-triggered interventions need first a better understanding of the cell-material interfaces that truly consider the patients’ biology. Few tools are available to study the interactions between polymers and dysfunctional soft tissue cells in vitro. Here, we propose polypropylene (PP) as a matrix to create microscale surfaces w/wo functionalization with an HBII-RGD molecule, a fibronectin fragment modified to include an RGD sequence for promoting cell attachment and differentiation. Metal mold surfaces were roughened by shot blasting with aluminum oxide, and polypropylene plates were obtained by injection molding. HBII-RGD was covalently attached by silanization. As a proof of concept, primary abdominal and vaginal wall fasciae fibroblasts from control patients were grown on the new surfaces. Tissue-specific significant differences in cell morphology, early adhesion and cytoskeletal structure were observed. Roughness and biofunctionalization parameters exerted unique and combinatorial effects that need further investigation. We conclude that the proposed model is effective and provides a new framework to inform the design of smart materials for the treatment of clinically compromised tissues.
Stability Analysis and Design of n-DOF Vibration Systems Containing Both Semi-Active and Passive Mechanical Controllers
Authors: Wang Kai , Xu Wei
Published: March 2024

This paper is concerned with the stability analysis and design of the n-DOF (n-degree-of-freedom) mass-chain vibration systems containing both semi-active and passive mechanical controllers. Based on Lyapunov’s stability theory, sufficient conditions are derived for the n-DOF vibration system containing a semi-active switched inerter and a passive mechanical network with the first-order admittance to be globally asymptotically stable. Furthermore, the optimization designs of a quarter-car vibration control system and a three-storey building vibration system are conducted together with the derived stability results, and the instability cases contradicting the stability conditions are presented for illustration. The optimization and simulation results show that the combination of semi-active and passive mechanical controllers in vibration systems can clearly enhance system performances in comparison with the conventional semi-active or passive control. The novelty of this paper is that the stability problem of a general n-DOF vibration system that simultaneously contains a semi-active controller and a first-order passive controller is investigated for the first time, where such a system combines the advantages of both semi-active and passive mechanical controllers. The investigations and results can provide an essential foundation for further exploring the stability problems of more general systems, and can be applied to the controller designs of many vibration systems in practice.