bluuebunny/crossref_metadata_2025 huggingface dataset:
The effects of feeding high concentrations of molasses and supplementing with nitrogen and unprotected tallow on intake and performance of dairy cows
Authors: Yan T. , Roberts D. J. , Higginbotham J.
Published: September 2010

A series of three replicated 3 × 3 Latin-square design experiments was carried out with lactating dairy cows (15 in experiments 1 and 3, and 18 in experiment 2). The objectives of these experiments were to investigate if the feeding of high concentrations of molasses would be detrimental to the performance or health of cows, and if the strategic supplementation of nitrogen or unprotected fat could enhance the efficiency of molasses feeding. In experiment 1, complete diets were based on grass silage with three inclusion rates of a liquid molasses-based supplement (Molaferm 20) to supply molasses dry matter (DM) of 125, 250 and 375 g/kg DM respectively. In experiment 2, the control diet contained (g/kg DM) 460 grass silage, 310 'molaferm 20' and 192 barley and 22 soya-bean meal, and was then supplemented with urea (12 g/kg DM) or soya-bean meal (177 g/kg DM). In experiment 3, the control diet was based on (g/kg DM) grass silage (460), molaferm 20 (310), barley (130), soya-bean meal (65) and oatfeed (20). Two concentrations, low and high, of unprotected tallow were added to the control diet (12·5 and 25·8 g/kg DM, respectively).

In experiment 1, cows suffered from some scouring on the highest molasses treatment, but recovered when changed to either the medium or low molasses treatment. However, there were no clinical symptoms of ill health observed during experiments 2 and 3. In experiment 1, animal intake and performance on the diets containing molasses DM of 125, 250 and 375 g/kg DM respectively were: total DM intake, 12·8, 16·2 and 18·6 kg/day (s.e.d. 1·03, P < 0·001); milk yield, 15·5,17·4 and 17·6 kg/day (s.e.d. 0·53, low v. medium or high, P < 0·01); fat concentration, 39·6, 39·7 and 40·0g/kg (s.e.d. 0·70, P>0·05); protein concentration, 31·6, 32·7 and 33·6g/kg (s.e.d. 0·22, P < 0·05). In experiment 2, supplementation of the control diet both with urea and soya-bean meal significantly increased DM intake (P < 0·01); milk yield (P < 0·01) and protein concentration in milk (P < 0·05), but had no significant effects on fat concentration in milk (P > 0·05). In experiment 3, there were no significant differences in DM intake between the three treatments. However, supplementation with both low and high concentrations of unprotected tallow significantly increased milk yield (P < 0·01) and significantly decreased fat (P < 0·01) and protein (P < 0·001) concentrations in milk.

It is concluded that lactating dairy cows could be given molasses up to 250 g/kg DM in grass silage-based complete diets without adverse effects on milk production or health. When a diet contained a high concentration of molasses (248 g/kg DM), supplementation with urea and soya-bean meal increased intake and milk production, and supplementation with unprotected tallow also increased milk yield, but fat and protein concentrations in milk were decreased.
The effect of immunization against somatostatin and β-agonist administration alone and in combination on growth and carcass composition in young steers
Authors: Dawson J. M. , Soar J. B. , Buttery P. J. , Craigon J. , Gill M. , Beever D. E.
Published: September 2010

The effect of active immunization against somatostatin and β-agonist administration alone and in combination on growth, carcass composition and endocrine responses was investigated in young steers given a pelleted diet of dried grass and barley (0·7: 0·3) ad libitum for 16 weeks. Immunization alone increased growth rate by proportionately 0·10 but had no effect on plasma growth hormone (GH) or insulin-like growth factor-1 (IGF-1) concentrations (P > 0·10). Carcass protein content was unchanged but fat content tended to be increased. This was possibly associated with increased (P < 0·10) plasma insulin concentrations observed in these animals. Cimaterol administration significantly increased growth rate (P < 0·05) and carcass weight (P < 0·01). Carcass protein content was significantly (P < 0·01) increased and carcass fat was significantly (P < 0·001) reduced. Administration of cimaterol to immunized animals resulted in an enhancement of the repartitioning effect of the pagonist although growth rates were intermediate between the separate treatments. The mechanism by which this was achieved is unclear. Plasma IGF-1 concentrations tended (P < 0·10) to be lower in these animals compared with controls. Plasma insulin concentrations were also reduced (P < 0·05) in these animals. Both immunized animals and those given cimaterol alone showed an increased GH response to a single intravenous injection of growth hormone releasing factor (GRF) (P < 0·05). Animals given the combined treatment however showed a reduced response suggesting that the effects were not mediated through direct changes in GH secretion. The improved lean:fat ratio in the carcass was observed to be mediated through an enhancement of the lipolytic response to the /J-agonist with only small effects on protein accretion. It is suggested that immunoneutralization of somatostatin may increase the sensitivity of adipose tissue to lipolytic stimuli. This could occur through changes in the secretion of gastrointestinal or pancreatic hormones as a result of neutralization of the inhibitory influences of somatostatin. Further work is required to clarify these effects.
The influence of terminal sire breed on carcass composition and eating quality of crossbred lambs
Authors: Ellis M. , Webster G. M. , Merrell B. G. , Brown I.
Published: September 2010

Carcass and eating quality characteristics of crossbred wether and female lambs sired by Charollais (C), Suffolk (S) and Texel (T) rams out of Mule ewes (Bluefaced Leicester ♂ × Scottish Blackface ♀ or Swaledale ♀J)were evaluated in a study carried out over a 3-year period in an upland flock. A total of 10 unrelated rams of each breed were used as sires. Lambs (no. = 280) were selected for slaughter at the same estimated subcutaneous fat level on four occasions within each year (i.e. at weaning in July and at approx. monthly intervals thereafter). Following slaughter, the left side of each carcass was physically dissected. A loin joint from lambs slaughtered in the 2nd and 3rd years of the study (no. = 184) was used to evaluate eating quality.

Suffolk-sired lambs were heavier at slaughter (S: 42·2; C: 41·0; T: 40·7 (s.e. 0·35) kg) but had similar killing-out proportions compared with the other two breeds (S: 444; C: 450; T: 452 (s.e. 26) g/kg). Texel-sired lambs had higher lean proportion (S: 543; C: 550; T: 567 (s.e. 4·4) g/kg) and lower total carcass fat proportion (S: 240; C: 242; T: 233 (s.e. 5·3) g/kg) compared with S and C sired lambs. Bone proportion was similar for C and T lambs but was higher for S (S: 199; C: 191; T: 191 (s.e. 2·1) g/kg). Lean: bone (S: 2·75; C: 2·90; T: 2·99 (s.e. 0·037)) and lean:fat (S: 2·38; C: 2·40; T: 2·54 (s.e. 0·034)) ratios were higher for T-sired lambs. Female lambs were lighter (40·4 v. 42·2 (s.e. 0·24) kg), and had proportionately more intermuscular fat than wethers (122 v.118 (s.e. 1·3) g/kg) but the sex differences for other tissue proportions were small. Carcass weights and total carcass fat proportions increased and lean proportions decreased across the four slaughter dates. There were no effects of breed or sex on eating quality. However, samples from lambs slaughtered at weaning were judged to be more tender than those slaughtered later in the season.
The effect of diet in late pregnancy on colostrum production and immunoglobulin absorption in sheep
Authors: O'Doherty J. V. , Crosby T. F.
Published: September 2010

One hundred and twenty-six twin-bearing ewes were given either formic acid (FA) treated grass silage or grass/ molassed sugar-beet pulp (MSBP) silage in order to investigate factors affecting colostrum yield and quality and the efficiency of immunoglobulin (IgG) absorption. The experiment commenced on day 91 of pregnancy and the diets consisted ofFA-treated silage (Tl), FA-treated silage + soya-bean meal (SB) (72), MSBP silage (T3), MSBP silage + SB (T4), FA-treated silage + MSBP (T5), FA-treated silage + MSBP + SB (T6) or FA-treated silage + 150 g/kg of a crude protein concentrate (T7). Soya-bean meal was offered only in the last 3 weeks of pregnancy aiming for a total crude protein intake of220 g per ewe per day. Blood samples were taken from lambs in order to measure serum Ig concentrations. Ewes were milked at 1 h,10h and 18 h post lambing. Daily metabolizable energy intakes of 6·8, 11·4, 9·6, 12·8, 10·5, 13·7 and 14·7 (s.e. 0·58) M] per ewe were recorded for Tl to T7 respectively over the last 3 weeks of pregnancy. Respective crude protein intakes of 72, 213, 110, 225, 109, 215 and 175 (s.e. 5·64) g per ewe per day were recorded for Tl to T7 over the last 3 weeks of pregnancy. The addition of protein increased colostrum yield at 1 h (P < 0·01), 10 h (P < 0·01) and 18 h (P < 0·01) post lambing and total yield of colostrum to 18 h (P < 0·001). There was no significant response (P > 0·05) in colostrum production during the first 18 h following protein supplementation when the basic diet was FA-treated silage. There was no difference (P > 0·05) in colostrum production during the first 18 h between ewes offered MSBP treated silage or MSBP supplemented silage. Ewes offered FA-treated silage produced less colostrum during the first 18 h compared with ewes offered either MSBP treated or supplemented silage (P < 0·05). The production of colostral IgG during the first 18 h after parturition was related to the amount of colostrum produced; the greater the colostrum production the greater the Ig production. There was a significant quadratic relationship between the total amount of IgG ingested per kg lamb birth weight during the first 18 h and lamb serum IgG at 24 h (R2= 0·4005;P< 0·0001). Lamb serum Ig level increased linearly with increasing colostrum IgG consumption up to 15 g/kg lamb birth weight. Proportionately about 0·17 of the IgG ingested was present in the lamb's circulation at 24 h. Ewe protein supplementation pre-lambing increased the lamb's efficiency to absorb colostral IgG (P < 0·05) during the first 24 h. In conclusion, the data show positive relationships between feeding regimes, colostrum production and IgG transfer.
Genetic resistance of different genotypes of sheep to natural infections with gastro-intestinal nematodes
Authors: Romjali Endang , Pandey V. S. , Gatenby R. M. , Doloksaribu M. , Sakul H. , Wilson A. , Verhulst A.
Published: September 2010

During a 3-year period, from April 1991 to December 1993, in a flock with four lambing periods annually, faecal samples from lambs at weaning at the age of 3 months (no. = 1756), were examined for gastro-intestinal nematode eggs by the McMaster method. Faecal samples were also examined at the age of 9 (no. = 170), 12 (no. = 157), 15 (no. = 253) and 18 (no. = 85) months from the females only. From June to December 1993 packed cell volume (PCV) was also examined from lambs at weaning. Seven genotypes were used in this study.

The faecal egg counts were affected by genotype, birth season, type of birth and rearing, sex and age. First generation cross of Sumatra × Barbados Blackbelly had lowest eggs per g of faeces (EPG) followed by Sumatra, St Croix, Sumatra × St Croix Fl and F2 and Sumatra × Java Fat tail. Lambs born in February-March when rainfall was low had lower EPG (V < 0·05) than those born in May-June or August-September. The EPG of lambs born and reared singly were significantly lower (P < 0·001) than those born twin, triplet or quadruplet. Female lambs had lower EPG than male lambs (P < 0·001). The EPG of lambs at 3 months were higher than at 9, 12, 15 or 18 months (P < 0·001).

PCV was affected by genotype, sex, type of birth and rearing and season of birth. There was a positive correlation between PCV and weaning weight of lambs and a negative correlation between EPG and weaning weight. These results show that the crosses of St Croix and Barbados Blackbelly with local Sumatra sheep are at least as resistant or more resistant to gastro-intestinal nematodes than pure Sumatra breed and therefore these breeds can be used in cross breeding programmes to improve the body size of local Sumatra sheep.
Ram-induced reproduction in seasonally anovular Corriedale ewes: MAP doses for oestrous induction, ram percentages and post-mating progestagen supplementation
Authors: Rodríguez Iglesias R. M. , Ciccioli N. H. , Irazoqui H.
Published: September 2010

Exploiting the ‘ram effect’ is an inexpensive alternative for out-of-season breeding in moderately seasonal breeds. For maximum efficacy in Corriedale sheep, oestrous ewes have to be introduced together with the teaser rams. Successful applications depend upon timely manipulation of the animals to induce oestrus in most ewes and to minimize the likelihood of successfully induced non-pregnant ewes returning to anoestrus during the mating period.

In the first of two experiments, the effects of two alternative ram: ewe ratios (1:6 or 1:12) and five different doses (0, 15, 30, 45 or 60 mg) of medroxy-progesterone acetate (MAP) applied in intravaginal sponges for 9 days were assessed on seasonally anovular adult Corriedale ewes. Control ewes (0 MAP) did not receive sponges, but were injected with 20 mg progesterone at ram introduction. A second experiment tested the efficacy of post-oestrus intravaginally applied MAP (30 mg; 7-day treatment starting on days 3 to 6 after oestrus) for improving reproductive variables of successfully induced ewes exposed to entire rams.

Proportions of ewes ovulating and ovulation rates (assessed through laparoscopy) did not differ significantly (P > 0·05) in ewes treated with various MAP doses (213/220 = 96·8% and 1·26 (s.e. 0·031) respectively) or injected with progesterone (56/58 = 96·6% and 1·21 (s.e. 0·058) respectively). The MAP content of the sponges did not affect (V > 0·05) the proportion of ewes ovulating (269/278), marked by the rams (197/213), returning to oestrus (81/197) or lambing among those not returning to first oestrus (87/119). More ewes were marked in the groups exposed to a higher ram percentage (79·7 to 70·6%; P < 0·001) but no other reproductive variable was affected by the ram: ewe ratio at ram introduction (P > 0·05). In the second experiment, post oestrus progestagen supplementation reduced the proportion of ewes returning to first oestrus from 27% to 16% (P < 0·05) but failed to improve lambing rate or the number of lambs born per ewe lambing. Thus, MAP doses as low as 0·25 of the commercial formulation (60 mg) induced oestrus in seasonally anovulatory Corriedale ewes exposed to rams and oestrous ewes. Increasing the ram: ewe ratio augmented the proportion of marked ewes but did not affect any other reproductive variable. Supplementing MAP after oestrus did not improve reproductive rate in Corriedale ewes induced to ovulate during the seasonal anoestrus.
Nutrition of draught oxen in semi-arid west Africa. 2. Effect of work on intake, apparent digestibility and rate of passage of food through the gastro-intestinal tract in draught oxen given crop residues
Authors: Fall A. , Pearson R. A. , Lawrence P. R. , Fernández-Rivera S.
Published: September 2010

Two experiments were conducted to investigate the relationships between work and intake and digestion of food by draught oxen given millet stover. In the first experiment, intake of millet stover, water intake, live weight, plasma concentrations of triiodothyronine, thyroxine and urea-nitrogen were measured in 18 animals that worked 0, 2 or 4 h/day in sequence during three 3-week experimental periods. Digestibility and rate of passage of food residues through the digestive tract were measured in a second experiment on 12 animals working either 0, 2-5 or 5 h/day in sequence during three 2-week experimental periods. Feeding behaviour was monitored on six animals working either 0, 2-5 or 5 h/day. Work did not affect intake of millet stover, apparent digestibilities and the rate of passage of digesta through the gastro-intestinal tract. This suggests that the nutrient supply from intake of roughages by ivorking oxen is unlikely to be sufficient to compensate for the extra energy expended during work. Food intake was affected by the quality of the millet stover offered. The level of intake of millet stover ivas proportional to the amount of leaves in the stover. Food intake increased also as work progressed. However, animals mobilized their body reserves to perform work. Animals consumed more water on working days than on days they were at-rest in shade. The heat stress that working animals were subjected to did not appear to interfere with their digestive function.
Responses of Romney sheep to selection for resistance or susceptibility to nematode infection
Authors: Morris C. A. , Vlassoff A. , Bisset S. A. , Baker R. L. , West C. J. , Hurford A. P.
Published: September 2010

Divergent breeding lines of Romney sheep, selected as lambs for consistently low or high faecal worm egg count (FEC) following natural multispecific challenge by nematode parasites, have been maintained at Wallaceville Animal Research Centre since 1979. From the start of the trial until 1992, 821 and 736 experimental lambs in lines selected for increased resistance (R) and increased susceptibility (S), respectively, were generated, with the use of 44 different sires. In order to assess genetic responses to selection, FEC and productivity data were analysed using restricted maximum likelihood procedures. By 1988 the two lines had diverged in average loge (FEC+100) by 0·67 log units, and by 1992 divergence had increased to 1·48 log units, representing 2·90 genetic standard deviations of divergence. No significant correlated responses were observed in live weights. However, by 1992 the mean score for breech soiling (dags) in the R line was proportionally 0·41 greater than in the S line (F < 0·001). Yearling fleece weight was proportionally 0·15 greater in S-line than in R-line animals when grazed together under identical levels of challenge (P < 0·001) but the lines did not differ significantly in fleece weight when they were grazed apart. The R line had significantly higher reproductive success than the S line (P < 0·05), as measured by lambs weaned per ewe mated (1·01 v. 0·92, respectively). It is concluded that selective breeding can change the degree of resistance or susceptibility to nematode infection in Romney sheep, and selection for productivity needs to be continued at the same time in any practical breeding programme.
The influence of heat production on voluntary food intake in growing pigs given protein-deficient diets
Authors: Ferguson N. S. , Gous R. M.
Published: September 2010

Ninety-six entire male Large White XLandrace pigs were assigned at 13 kg to one of six dietary crude protein (P) treatments (230 g/kg (P1), 201 g/kg (P2), 178 g/kg (P3), 151 g/kg (P4), 125 g/kg (P5), 93 g/kg (P6)) and one of four temperatures (T) (no. = 4) (18°C, 22°C, 26°C, 30°C), and were given food ad libitum until slaughter weight of 30 kg. At all temperatures gut fill was a constant proportion of food intake (Fl) (1·56) but this ratio varied with different protein concentrations. Food intake increased with decreasing temperature and with decreasing protein content to a maximum rate on P4 (1·347 kg) whereafter FI declined. There was a linear decrease in average daily gain (ADG) with decreasing protein content while temperature had a significant curvilinear effect on ADG and food conversion ratio (FCR) with maximum ADG (0·680 kg/day) at 26°C. Body protein content decreased as the dietary protein concentration declined below P3 and there was a corresponding increase in lipid content. Temperature had no effect on body protein content but had a significant effect on lipid content. Similar trends occurred in the rate of protein (PR) and lipid (LR) retention with maximum PR (117·1 g/day) attained on PI, P2 and P3. Protein and temperature had a significant effect on total heat loss (THL). Maximum THL occurred in the protein treatment that resulted in pigs consuming maximum FI. The efficiency of protein utilization increased with increasing temperature but the response was dependent on the protein supply. It is concluded that on low protein diets pigs increase their Fl to maintain potential protein growth until a point is reached where the animal can no longer compensate and FI will decline. The extent of the compensation will depend on the amount of heat the animal can lose which in turn is dependent on the environmental temperature.
Relationship of body condition score and live weight with body composition in mature Churra ewes
Authors: Frutos P. , Mantecón A. R. , Giráldez F. J.
Published: September 2010

Thirty-five mature Churra ewes, ranging in live weight from 30·3 to 52·6 kg and in body condition score from 1·25 to 4·00 were used to study the relationship between body condition score (BCS), live weight (LW) and body composition and fat distribution in ewes of this breed, which is one of the major sheep breeds of northern Spain. The procedure at slaughter and at subsequent dissection was designed to partition each body into two components, carcass and ‘non-carcass’. Right side carcasses and ‘non-carcass’ components were used to analyse the chemical composition. From the left side of the lumbar region a joint was cut and dissected into muscle, bone, subcutaneous and intermuscular fat. According to the results obtained, omental fat represented the highest proportion of total internal fat regardless of the level of fatness. Distribution of internal fat was similar to that observed in other milk production breeds. Regressions on LW explained more of the variation than those on BCS for individual internal fat depots and chemically determined ‘non-carcass’ fat. The prediction of total body fat afforded by LW was better than that provided by BCS. The subcutaneous and intermuscular fat depots in the lumbar joint were well correlated with BCS, carcass fat and total fat in the body, validating the use of this region for assessing BCS in Churra ewes. Nevertheless, the correlation coefficient with the omental depot was not statistically significant. The results of this study suggest that BCS was not as accurate for estimating body composition and fat depots in mature Churra ewes as has been shown previously in other breeds. The single most effective prediction index was LW. However, the utilization of both BCS and LW together provided more accurate estimations.
The effect of the introduction of the Thoka gene for fecundity on lamb production from Cheviot ewes
Authors: Russel A. J. F. , Alexieva S. A. , Elston D. A.
Published: September 2010

A flock of Cheviot ewes carrying the Thoka gene for fecundity was established in southern Scotland through the importation of frozen semen from two Icelandic rams. Ewes considered to be probable carriers of the Thoka gene were identified on the basis of measurements of ovulation rate as juveniles at 1·5 years of age and again as adults. Analysis of 3 years' records of 8926 Cheviot lambings and lambings of 351 Icelandic × Cheviot ewes considered to be probable carriers showed that fecundity increased with age in both genotypes, the number of lambs born per ewe mated being 1·07, 1·19, 1·31 and 1·41 in the Cheviots and 1·55, 1·74, 2·33 and 2·30 in the Icelandic-Cheviot crosses at first to fourth lambings respectively. The apparent effect of the crossbreeding on fecundity thus also increased with age, values at first to fourth lambings being 0·48, 0·55, 1·02 and 0·89 lambs per ewe mated, giving a weighted mean value of 0·61 (1·20 v. 1·81 lambs per ewe mated in Cheviot and Icelandic × Cheviot ewes respectively). There was a positive effect of the proportion of Icelandic ancestry on the number of lambs born per ewe mated and thus the observed increases in fecundity, although attributed principally to the effects of the Thoka gene, are also due in part to other genetic factors introduced from the Icelandic sheep in the crossbreeding programme. Lamb mortality to 6 weeks of age was only marginally higher in Icelandic × Cheviots (0·10) than in Cheviots (0·08). The increase in fecundity was achieved without an excessive proportion of large litters (<0·02 of probable carriers gave birth to quadruplets). The results demonstrate that the introduction of the Thoka gene to breeds such as the Cheviot can increase fecundity and hence the potential for more efficient lamb production.
The effect of nutrition on fibre growth in the alpaca
Authors: Russel A. J. F. , Redden H. L.
Published: September 2010

Twelve adult male alpacas were given either 0·67 (low) or 2·0 (high) × assumed maintenance requirements for a period of 6 weeks after which time each was transferred to the alternative level of nutrition for a further 6 weeks. Fibre samples were taken from two 10-cm2 areas on the mid-side position of each animal at 2, 6, 8 and 12 weeks, and measurements of fibre weight, yield (clean fibre weight/raw fibre weight), fibre diameter and fibre length made on the samples collected at weeks 6 and 12. The higher level of feeding resulted in higher clean fibre weight (low = 0·42 (s.e. 0·03); high = 0·53 (s.e. 0·04)mg/cm2 per day, P < 0·001) and fibre growth rate (low = 186 (s.e. 10); high = 223 (s.e. 14) yon/day, P < 0·05). Changes in yield (low = 0·917 (s.e. 0·006); high = 0·929 (s.e. 0·009)) and mean fibre diameter (low = 31·4 (s.e. 1·9); high = 32·1 (s.e. 1.6) \xm) were not statistically significant. Calculations showed that the increased weight of fibre attributed to the higher level of nutrition could be explained in terms of the observed increases in fibre-length and diameter but that, unlike the sheep in which the ratio fibre length: diameter remains relatively constant under varying nutritional regimes, the effect of nutrition in the alpaca has a proportionally larger effect on fibre length than on fibre diameter.
Predicting the effects of animal variation on growth and food intake in growing pigs using simulation modelling
Authors: Ferguson N. S. , Gous R. M. , Emmans G. C.
Published: September 2010

All pig nutrition models to date predict growth responses of either an individual animal or the average animal of a given population over time. Translating the predicted nutrient requirements from the average animal to the population introduces a number of errors as the cause-and-effect response of the average animal is different from the population response. To overcome the problem of estimating the requirements for a given population using models it is necessary to simulate a number of individuals representative of a population and then average these results. This approach however, requires a knowledge of those animal characteristics that vary between individuals and the nature of their distribution. In this paper a scaled growth rate constant (B*), protein weight at maturity (Pm) and the ratio of lipid to protein at maturity (LPRm) are the parameters used to define an individual animal. As no data existed from which the nature of the distribution of B*, Pm and LPRm can be estimated for pigs of different strains and sexes, and due to the impracticality of determining this variability by experimentation, a simulation model was used to estimate the variations within each parameter. In addition this paper quantifies the subsequent effects these distributions have on the genetic variability of average daily gains (ADG) and daily food intake (TI) over a live-weight range of 25 to 90 kg. Comparisons were made between the genetic variation determined by modelling and those published in the literature. The results indicated coefficients of variation for B*, Pm and LPRm of 0·01, 0·05 and 0·10, respectively. An increase in the variability of all three parameters resulted in an increase in the variation in ADG whilst only an increase in the variation of B* and LPRm affected the distribution of FI.
Selection of farm animals for non-linear traits and profit
Authors: Meuwissen T. H. E. , Goddard M. E.
Published: September 2010

According to animal breeding theory, profit after, say, 10 generations of selection is maximized when the usually non-linear profit function is approximated by a linear breeding goal where the linearization is at the population mean in generation 10 and the linear breeding goal is subsequently predicted by a linear index for which the animals are selected. The prediction of the population mean at generation 10 requires linear relationships among the traits that constitute the non-linear profit, because otherwise this prediction becomes very complicated.

A non-linear index is proposed that simply estimates the non-linear goal H =f(u) by Ĥ =f(û), where u = vector of genetic values for the traits and u is its (BLUP) estimate. This non-linear index does not require predictions of (future) population means and does not require linearly related traits.

To test these indices a simple meat production example was constructed where the non-linearity between the traits was due to the competition between weight and probability of survival for the same resources from food intake. In the model selection for weight and, in particular, for weight over costs (mainly food) led to reduced profits due to large reductions of survival rates. Although, the example was oversimplified, this should provide a warning for the use of oversimplified breeding goals, e.g. fitness traits may reduce by more than expected from base population genetic parameters.

When probability of survival and weight were measured, a non-linear index of these non-linear traits gave the greatest genetic gains. Failure to update genetic parameters each generation severely reduced genetic gain and, if linear indices were used, it was also important to update the economic weights. When probability of survival was measured, profit could be calculated on each animal and included as a trait in the calculation of estimated breeding value. This yielded high genetic gain and did not require updating of genetic parameters or economic weights.
The effect of melatonin treatment in combination with progestagen pessaries on reproductive performance of ewes during the anoestrous period
Authors: Laliotis V. , Vosniakou A. , Lymberopoulos A. , Doney J. M. , Kouimtzis S.
Published: September 2010

The objective of this study was to compare the effects of melatonin implants combined with intravaginal, progestagen-impregnated pessaries with the conventional treatment of progestagen pessaries followed by pregnant mare serum gonadatrophin (PMSG) on the reproductive performance of ewes during the anoestrous period.

A commercial flock of 179 seasonally anoestrous Chios crossbred ewes was used. The ewes within the flock were randomly allocated to one of two groups. Ewes of group MP (melatonin + pessaries) received melatonin implants on 20 March (50 days before the target breeding date of 10 May) and were treated with intravaginal progestagen pessaries 35 days later (25 April). Ewes of group PP (pessaries + PMSG) were also treated with pessaries on 25 April. After 14 days the pessaries were removed from both groups and 500 i.u. PMSG was injected (i.m.) into ewes of group PP only. Fertile rams were introduced into both groups 24 h after sponge removal. Pregnancy rate and litter size were determined by ultrasound scanning 80 days after ram introduction.

Pregnancy rate at first oestrus was significantly higher in group MP than in group PP (57 v. 40%, P < 0·05). The overall conception rate during two oestrous cycles was significantly higher in group MP than in group PP (78 v. 59%, P < 0·02). The groups did not differ significantly in the mean number of foetuses per pregnant ewe that conceived during the first or second oestrus after pessary removal (1·78 and 1·87, respectively). The total number of foetuses per treated ewe was higher in group MP than in group PP (1·39 v. 1·10) as a consequence of the difference in pregnancy rate.
Differences in microbial fermentation of barley straw induced by its treatment with anhydrous ammonia
Authors: Fondevila M. , Muñoz G. , Castrillo C. , Vicente F. , Martín-Orúe S. M.
Published: September 2010

The effect of ammonia treatment of straw on both the rumen environment and the extent of its microbial fermentation was studied. Four rumen cannulated sheep were randomly given 700 g/day of untreated straw plus urea (US), ammonia-treated straw (TS) and alfalfa hay (AH) in a change-over design with three periods. Rumen pH was lower and ammonia-nitrogen and total volatile fatty acid (VFA) concentrations were higher (P < 0·001) with AH than with US or TS. With the straw diets, TS promoted a lower pH than US (P < 0·05), but differences were less than 0·3 units and the mean pH was never below 6·5. There were no differences between the straw diets in ammonia-nitrogen or VFA concentration (P > 0·05). When untreated barley straw (BS) and treated straw (TS) were incubated in situ disappearance of dry matter (dDM) at 12, 24 and 48 h (P < 0·01) and neutral-detergent fibre (dNDF) at 48 h (P < 0·001) were higher with TS. In vitro incubation showed a higher gas production with TS only after 36 h (P < 0·05) whereas gas from BS fermentation was higher up to 14 h (P < 0·05). Among diets, dDM, dNDF and gas production with US were numerically higher than with TS or AH throughout, although few significant differences were observed, except for a higher dDM at 12 (P < 0·01) and 24 (P < 0·10) h and a higher dNDF at 12 h (P < 0·10). Particle-associated enzymes were extracted from BS and TS incubated in the rumen for 4, 8, 22 and 24 h. Results ofxylanase and cellulase activities support those of straw incubation, with a drop between 4 and 8 h in TS diet. The concentration of residual phenolics per unit of incubated straws after 12 and 24 h show that phenolics release to the media was higher with the TS diet. Daily changes of phenolic concentration into rumen liquid was also higher with TS than with US (P < 0·001). The increased release of straw phenolics by ammoniation reduced the potential for rumen degradation of straw, mainly in the first hours of the fermentation period.
Parity influences the utilization of exogenous glucose in suckler anoestrous Charolais beef cows
Authors: Ponter A. A. , Grimard B. , Humblot P. , Novak N. , Khireddine B. , Sauvant D. , Thibier M. , Mialot J.-P.
Published: September 2010

This work was designed to study the influence of parity (multiparous (MP, no. = 9) or primiparous (PP, no. = 10)) and feeding level (control diet (CD; 1·00) or restricted diet (RD; proportionately 0·70 of requirements) in a 2×2 factorial design on the utilization of exogenous glucose in the suckler anoestrous Charolais beef cow. Lack of ovarian activity was confirmed by low circulating progesterone concentrations measured in weekly blood samples taken after parturition. An injection of glucose (1·5 mmol/kg live weight) lasting 20 min was given 30 days and 50 days post partum. Blood samples were collected before and for 120 min after the start of the injection and plasma insulin, glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate and urea were measured. Although plasma glucose concentrations were not significantly different between treatment groups in the period immediately after the injection of glucose, CD PP animals had higher plasma insulin levels than CD MP animals (at 25 min: 596·4, 283·8, 435·1 and 309·6 pmol/l,P< 0·05 for groups PP CD, MP CD, PP RD and MP RD respectively). Plasma NEFA concentrations were initially higher in the RD groups compared with the CD groups but were reduced by the glucose injection (P < 0·001). However, the PP RD cows took longer to respond to the exogenous glucose compared with the MP RD cows (at 25 min: PP RD: 133·2 and MP RD: 57·5 eq/l,P< 0·05). In conclusion, suckler anoestrous PP cows appear to require higher plasma insulin levels than MP cows to metabolize exogenous glucose and underfed PP cows were slower to reduce plasma NEFA after glucose injection than underfed MP cows.
Selection for high and low prolificacy in Cambridge sheep
Authors: Ap Dewi I. , Owen J. B. , Axford R. F. E. , Beigi-Nassiri M. T.
Published: September 2010

Previous reports have suggested the presence of a major gene influencing prolificacy in the Cambridge sheep breed. To estimate the effect of such a gene, high and low prolificacy groups were established in a Cambridge sheep flock between lambing years 1990 and 1993. In 1990-1991 ovulation rate (OR) was used as the basis for allocating ewes into groups but for 1992-1993 litter size was used also as a secondary selection criterion. In 1990 and 1991 small groups (each with one ram) of extreme phenotype were formed. In 1992 and 1993, six high and six low groups were formed using all available ewes, increasing the number of observations but with less selection pressure for high and low prolificacy. Results from the groups were interpreted on the basis of a major gene with additive effect resulting in three distinct genotypes (CC, Cc and cc). It was assumed, because of the selection method adopted, that CC ewes were exclusively in the high groups, heterozygotes (Cc) were distributed between the high and low groups and that cc ewes were exclusively in the low groups. In 1990-1991 there was a difference in OR of 4·0 between ewes allocated to the high and low groups. In 1992-1993 the difference was 1·9. Litter size differences between groups averaged 0·73. Whilst the high group progeny had higher OR, the differences between groups were less than differences observed between groups based on selected dam records, possibly a reflexion of the young age at which progeny records were collected. Differences between the high and low groups suggest a gene effect for adult ewes of approximately 2·0, with expected OR, above a basal level of 2·0, of 4·0 and 6·0 for heterozygous and homozygous carriers respectively. The effect of the gene in young ewes (predominantly 1 to 2 years) was approximately 0·8.
Genetic parameters for lamb weight at different ages and wool production in Baluchi sheep
Authors: Yazdi M. H. , Engström G. , Näsholm A. , Johansson K. , Jorjani H. , Liljedah L.-E.
Published: September 2010

The Baluchi breed is the most common native breed of Iran adapted to harsh environments in the eastern parts of the country. The data used in the present study, collected from two research flocks at the Abbasabad sheep breeding station in north-east Iran, included 20 534 animals descended from 363 sires, 5992 dams, 282 maternal grandsires, and 2865 maternal granddams during the period 1966 to 1989. The traits recorded were: birth weight (BW), weaning weight (WW), weight at 6 months (W6), weight at 12 months (YW), pre-weaning gain (WG), post-weaning gain (PWG), lamb fleece weight (LFW), ewe fleece weight sheared before first joining (FW1) and adult ewe fleece weight (FW). Genetic parameters, estimated with restricted maximum likelihood and a two-trait animal model, were similar in the two flocks. Direct heritabilities for the various body weight traits were moderate and varied between 0·13 and 0·32, while the maternal heritabilities were low and varied between 0·01 and 0·12. Direct and maternal genetic correlations between WW and weights at later ages were moderate to high (0·59 to 0·96). Direct heritabilities of weight gain measures varied between 0·12 and 0·19, while no significant maternal influence on either of these weight gain measures could be detected. The estimates of direct genetic correlation between WG and PWG were positive and varied between 0·54 and 0·74, while negative maternal genetic correlation (0·17 on average) between WG and PWG was detected. For LFW, direct heritability was low and no maternal heritability could be shown. For FW1, both direct and maternal genetic influences were demonstrated (0·07 to 0·26). Direct genetic correlation between LFW and FW1 was very low and close to zero, while maternal genetic correlation was positive and relatively high (0·72 on average). The relative contributions to phenotypic variance from variance components due to common environmental effects ranged from 0·01 to 0·15 for all traits. The repeatability of FW was low (0·03 to 0·12).
Timing of melatonin treatment: differential effects on oestrus and coat growth in goats
Authors: Forsyth I. A. , Gebbie F. E. , Arendt J.
Published: September 2010

To avoid winter scarcity of fresh goat milk, simple methods of advancing the season of kidding would be commercially valuable. A combination of long-day light treatment followed by melatonin is successful but other aspects of seasonality including coat growth are also reset. To investigate whether effects on breeding season and coat growth can be dissociated, British Saanen dairy goats (no. = 30) were randomly allocated to one of six groups. Control goats were untreated. Goats in the other five groups received 2 months of treatment with 20 h light: 4 h darkness (20L: 4D) and were then given 3 mg melatonin orally at 16.00 hfor 2 months. The treatments started on 11 January (group 1), 29 March (group 2), 14 June (group 3), 30 August (group 4) and 8 November (group 5). Weekly blood samples were taken for the measurement of progesterone to monitor ovarian activity and prolactin. Side patches (10 cm ×10 cm) were clipped monthly from alternate sides and weighed to measure coat growth. The onset of ovarian activity was advanced from a median date of 15 November in controls to 24 May in group 1 (P < 0·01), 16 August in group 2 (P < 0·05) and 18 October in group 3 (? < 0·05). Groups 4 and 5 showed no significant change in the onset of oestrus (median onset dates 8 November and 1 November, respectively). Group 1 goats came into season again with controls in November. In all except group 5, treatment interacted with time to affect significantly the growth of the coat. The pattern of coat growth was most altered (P < 0·001) in groups 1 and 2. This was associated with effects on plasma prolactin concentrations of light stimulation and melatonin suppression. Group 1 goats resynchronized with control goats to show a coat of normal weight in the winter following treatment. Division of milking goat herds into spring and autumn kidding groups is, therefore, a practical possibility, but effects on overall milk yield require study.
Responses of young calves, on two levels of feeding, to transportation
Authors: Schrama J. W. , Heetkamp M. J. W. , Verstegen M. W. A. , Schouten W. G. P. , van der Veen F. , Helmond F. A.
Published: September 2010

The effect of transportation on energy metabolism in young calves was assessed at two feeding levels by indirect calorimetry during a 5·5-day experimental period. Twenty-six Holstein-Friesian male calves were assigned in a 2 × 2 factorial arrangement of treatments. Calves were fed below or near the maintenance requirements (270 or 420 k) metabolizable energy per kg M0·75 per day). At 5 days of age, 13 calves were transported. The applied transportation procedure was aimed to mimic common transport of young calves. The other 13 calves were controls. Metabolizability of dietary energy was low (0·808), but similar for control and transported calves. Despite the similar salivary cortisol concentrations after transportation, heat production was higher by 23 kJ/kg M0·75 per day in transported than in control calves. Calves were not in a steady-state regarding their energy metabolism. Heat production decreased with time. Both transportation and feeding level influenced the decline in heat production with time. Only during the first 3 days after transportation was heat production enhanced, suggesting a relatively short-term effect of transportation in young calves. The impact of transportation on heat production and time related alterations in heat production were not related to the calfs activity.
Phenotypic variation in residual food intake of mice at different ages and its relationship with efficiency of growth, maintenance and body composition
Authors: Archer J. A. , Pitchford W. S.
Published: September 2010

Food intake and body weight of 119 mice was measured from 3 to 18 weeks of age. Residual food intake was calculated for each week as the variation in food intake independent of variation in weight gain, weight maintained and sex. Growth efficiency and maintenance requirement were calculated by fitting curves to data from 3 to 18 weeks. The repeatability of residual food intake was low in young mice, but increased as they matured. Growth efficiency was correlated with residual food intake in very young mice. Residual food intake was not correlated with maintenance requirement in young mice, but as mice matured the correlation of residual food intake with maintenance requirement increased to 0·6. Body composition at maturity was correlated with residual food intake and maintenance requirement of mature mice, but a large proportion of the variation in residual food intake and maintenance requirement was independent of body composition. The results suggest that the age at which residual food intake is measured is important if it is to be used as a criterion for selection for efficiency.
Evaluation of milk allantoin excretion as an index of microbial protein supply in lactating dairy cows
Authors: Shingfield K. J. , Offer N. W.
Published: September 2010

The potential of milk allantoin as an index ofmicrobial protein supply was evaluated in two experiments conducted with 12 multiparous Holstein-Friesian dairy cows that examined the effects of altering the supply of metabolizable energy (ME) and fermentable metabolizable energy (FME). In the first experiment, late lactation cows received a 14·3 kg dry matter (DM) per day basal diet consisting of (g/kg DM) chopped barley straw (415), soya-bean meal (322), molassed sugar-beet pulp (197), molasses (33), urea (17) and a vitamin and mineral supplement (17) for a 21-day co-variance period. During four 16-day periods, six treatments were allocated randomly to cows, consisting of potato starch (1, 2 or 3 kg DM per day) or fat supplements (0·64, 1·27 or 1·91 kg DM per day). In the second experiment, early lactation cows received 40 kg/day (fresh weight, (FW)) of silage (307 g/kg toluene-corrected dry matter, 149 g/kg DM crude protein and 11·6 MJ/kg ME). During three 21-day periods, four treatments were evaluated consisting of supplements of either 4·1 (LI) or 8·1 (12) kg fresh weight per day of a low fat concentrate (acid hydrolysis ether extract (AHEE) 45 g/kg DM) or 3·8 (HI) or 7·5 (H2) kg fresh weight per day of a high fat concentrate (AHEE 110 g/kg DM). Both experiments showed individual cow milk allantoin concentration or excretion to be poorly correlated with urinary purine derivative excretion or calculated microbial protein supply. Use of treatment mean (TM) values dramatically improved these relationships. For pooled TM (no. = 10) values from both experiments, close relationships existed between milk allantoin excretion and concentration with milk yield (r values 0·991 and 0·883, respectively). Auto-correlation with milk yield appeared to account for milk allantoin excretion and concentration being highly correlated with urinary purine derivative excretion (r values 0·908 and 0·934, respectively) and calculated microbial protein supply (r values 0·938 and 0·945, respectively). Current experimental data indicates that measurement of milk allantoin is not a reliable indicator of microbial protein supply for individual cows.