bluuebunny/crossref_metadata_2025 huggingface dataset:
Step-by-Step Growth of HKUST-1 on Functionalized TiO2 Surface: An Efficient Material for CO2 Capture and Solar Photoreduction
Authors: Di Credico Barbara , Redaelli Matteo , Bellardita Marianna , Calamante Massimo , Cepek Cinzia , Cobani Elkid , D’Arienzo Massimiliano , Evangelisti Claudio , Marelli Marcello , Moret Massimo , Palmisano Leonardo , Scotti Roberto
Published: August 2018

The present study reports on a simple preparation strategy of a hybrid catalyst, TiO2/HKUST-1, containing TiO2 anatase nanoparticles (NPs) with tailored morphology and photocatalytic activity coupled with a porous metal-organic framework (MOF), namely HKUST-1, as an advanced material for the CO2 photocatalytic reduction. In detail, TiO2/HKUST-1 catalyst was prepared via an easy slow-diffusion method combined with a step-by-step self-assembly at room temperature. The growth of crystalline HKUST-1 onto titania surface was achieved by functionalizing TiO2 nanocrystals, with phosphoesanoic acid (PHA), namely TiO2-PHA, which provides an intimate contact between MOF and TiO2. The presence of a crystalline and porous shell of HKUST-1 on the TiO2 surfaces was assessed by a combination of analytical and spectroscopic techniques. TiO2/HKUST-1 nanocomposite showed a significant efficiency in reducing CO2 to CH4 under solar light irradiation, much higher than those of the single components. The role of MOF to improve the photoreduction process under visible light was evidenced and attributed either to the relevant amount of CO2 captured into the HKUST-1 porous architecture or to the hybrid structure of the material, which affords enhanced visible light absorption and allows an effective electron injection from TiO2-PHA to HKUST-1, responsible for the photochemical reduction of CO2.
Adsorption Synthesis of Iron Oxide-Supported Gold Catalyst under Self-Generated Alkaline Conditions for Efficient Elimination of Carbon Monoxide
Authors: Pan Feng , Zhang Weidong , Ye Yuxiao , Huang Yixuan , Xu Yanzhe , Yuan Yufeng , Wu Feng , Li Jinjun
Published: August 2018

Goethite- and hematite-supported highly dispersed gold catalysts for carbon monoxide oxidation were synthesized by gold precursor adsorption onto the support materials in self-generated alkaline solutions. The support materials were prepared by reacting iron nitrate with excess sodium hydroxide. The residual minor alkali incorporated into the support could provide suitable alkaline conditions at approximately pH 8 for the hydrolysis of tetrachloroaurate anions and the subsequent adsorption process. Gold species underwent autoreduction to achieve activation during the synthesis. An increase in pH or temperature to 80 °C decreased the gold loading of the catalysts. The optimal catalysts could achieve complete oxidation of carbon monoxide at −20 °C.
Quality Control of Global Horizontal Irradiance Estimates through BSRN, TOACs and Air Temperature/Sunshine Duration Test Procedures
Authors: Ameen Bikhtiyar , Balzter Heiko , Jarvis Claire
Published: August 2018

Solar Radiation (SR) data are required for many disciplines and applications. The ground measurement of SR data is hampered by technical and operational errors. Therefore, several approaches have been developed to detect these errors. This study aimed to compare two quality tests of hourly Global Horizontal Irradiance (GHI) estimates through the Baseline Surface Radiation Network (BSRN) of the World Meteorological Organization (WMO) and Top of Atmosphere irradiance and Clear sky (TOACs) on a horizontal plane. Each of these tests has a threshold to pass data, which leads to different results. A newly developed quality test method is presented that uses Sunshine Duration (SD) and Air Temperature (AT) to check hourly GHI and is applied to data from 20 meteorological stations in northeast Iraq. The new method was validated using independent high quality data from six stations in various regions with the same climate regime. The method consists of several tests that compare ground data with upper and lower limits of radiation at the top of the atmosphere, using a clear sky radiation model and the relation between SD and AT with SR to determine data values of dubious quality. The rate of error flags generally range from 1% to 27%. The findings show that SD and AT can be used to support other quality tests and to detect nearly 2% additional dubious data values compared to BSRN and TOACs tests. The SD test tends to work like a consistency check but AT does not work like that according to the validation result. However, AT can be used to test the plausibility of data. The argument for using AT in this study may be impractical for other climate conditions. The results suggest that a combination of tests can lead to a better quality of ground data, especially when the components of SR are unviable. Using climate variables for further checks is another possibility.
The Impact of Biosurfactants on Microbial Cell Properties Leading to Hydrocarbon Bioavailability Increase
Authors: Kaczorek Ewa , Pacholak Amanda , Zdarta Agata , Smułek Wojciech
Published: August 2018

The environment pollution with hydrophobic hydrocarbons is a serious problem that requires development of efficient strategies that would lead to bioremediation of contaminated areas. One of the common methods used for enhancement of biodegradation of pollutants is the addition of biosurfactants. Several mechanisms have been postulated as responsible for hydrocarbons bioavailability enhancement with biosurfactants. They include solubilization and desorption of pollutants as well as modification of bacteria cell surface properties. The presented review contains a wide discussion of these mechanisms in the context of alteration of bioremediation efficiency with biosurfactants. It brings new light to such a complex and important issue.
Investigation of Approaches to Control the Compositions of Zn(Se,OH) Buffers Prepared by Chemical Bath Deposition Process for Cu(In,Ga)Se2 (CIGS) Solar Cells
Authors: Huang Chia-Hua , Jan Yueh-Lin , Chuang Wen-Jie , Lu Po-Tsung
Published: August 2018

We deposited zinc-based films with various ammonia (ammonium hydroxide; NH4OH) and selenourea concentrations, at the bath temperature of 80 °C, on soda-lime glass substrates using the chemical bath deposition (CBD) process. We analyzed the results using X-ray photoelectron spectroscopy (XPS), which showed binding energies of zinc, selenium, and oxygen. The as-deposited films, containing zinc selenide, zinc oxide, and zinc hydroxide, were also verified. The films prepared in this investigation can be referred to a zinc compound, characterized as Zn(Se,OH). A conformal coverage of the Zn(Se,OH) films, with the smooth surface morphologies, was obtained by optimizing the ammonia or selenourea concentrations in the deposition solutions. The Zn(Se,OH) films had a preferred (111) orientation, corresponding to a cubic crystal structure. The bandgap energies of the as-deposited Zn(Se,OH) films were determined from the optical absorption data, suggesting a dependence of the bandgap energies on the atomic percentages of ZnSe, Zn(OH)2 and ZnO in the films. The same variation tendency of the compositions and the bandgap energies for the films, deposited with an increment in the ammonia or selenourea concentrations was achieved, attributing to the facilitation of ZnSe formation. These results show that the compositions, and therefore the bandgap energies, can be controlled by the ammonia concentrations, or selenourea concentrations.
Development and Validation of Numerical Models for Evaluation of Foam-Vacuum Insulation Panel Composite Boards, Including Edge Effects
Authors: Biswas Kaushik
Published: August 2018

A combined finite element analysis (FEA) and experimental validation approach to estimating effective edge conductivities of vacuum insulation panels (VIPs) embedded in foam-VIP composites is presented. The edge conductivities were estimated by comparing the simulation results with measurements of small-scale (0.61 × 0.61 m) foam-VIP composites and using an error minimization method. The two composites contained multiple VIPs that were butt-jointed with each other in one composite and separated by foam insulation in the other. Edge conductivities were estimated by considering the neighboring materials, i.e., whether the VIPs were adjacent to other VIPs or foam insulation. Models incorporating the edge conductivities were then used to simulate additional small- and large-scale (2.44 × 1.22 m) composites for validation and evaluation of the overall thermal transmission properties. The simulations used either the same boundary conditions as the experiments or used the experimental parameters to define the appropriate boundary conditions.
Biodiesel by Transesterification of Rapeseed Oil Using Ultrasound: A Kinetic Study of Base-Catalysed Reactions
Authors: Encinar José María , Pardal Ana , Sánchez Nuria , Nogales Sergio
Published: August 2018

The objective of this work was to study the acceleration that ultrasound causes in the rate of biodiesel transesterification reactions. The effect of different operating variables, such as ultrasound power, catalyst (KOH) concentration and methanol:oil molar ratio, was studied. The evolution of the process was followed by gas chromatography, determining the concentration of methyl esters at different reaction times. The biodiesel was characterized by its density, viscosity, saponification and iodine values, acidity index, water content, flash and combustion points, cetane index and cold filter plugging point (CFPP), according to EN 14214 standard. High methyl ester yield and fast reaction rates were obtained in short reaction times. Ultrasound power and catalyst concentration had a positive effect on the yield and the reaction rate. The methanol:oil molar ratio also increased the yield of the reaction, but negatively influenced the process rate. The reaction followed a pseudo-first order kinetic model and the rate constants at several temperatures were determined. The activation energy was also determined using the Arrhenius equation. The main conclusion of this work is that the use of ultrasound irradiation did not require any additional heating, which could represent an energy savings for biodiesel manufacture.
Multifractal Characteristics and Classification of Tight Sandstone Reservoirs: A Case Study from the Triassic Yanchang Formation, Ordos Basin, China
Authors: Jiang Zhihao , Mao Zhiqiang , Shi Yujiang , Wang Daxing
Published: August 2018

Pore structure determines the ability of fluid storage and migration in rocks, expressed as porosity and permeability in the macroscopic aspects, and the pore throat radius in the microcosmic aspects. However, complex pore structure and strong heterogeneity make the accurate description of the tight sandstone reservoir of the Triassic Yanchang Formation, Ordos Basin, China still a problem. In this paper, mercury injection capillary pressure (MICP) parameters were applied to characterize the heterogeneity of pore structure, and three types of pore structure were divided, from high to low quality and defined as Type I, Type II and Type III, separately. Then, the multifractal analysis based on the MICP data was conducted to investigate the heterogeneity of the tight sandstone reservoir. The relationships among physical properties, MICP parameters and a series of multifractal parameters have been detailed analyzed. The results showed that four multifractal parameters, singularity exponent parameter (αmin), generalized dimension parameter (Dmax), information dimension (D1), and correlation dimension (D2) were in good correlations with the porosity and permeability, which can well characterize the pore structure and reservoir heterogeneity of the study area, while the others didn’t respond well. Meanwhile, there also were good relationships between these multifractal and MICP parameters.
Conceptual Synthesis of Speed Increasers for Wind Turbine Conversion Systems
Authors: Saulescu Radu , Neagoe Mircea , Jaliu Codruta
Published: August 2018

Most wind turbines (WT) are of the single-rotor type, which means they are simple, reliable and durable, but unlikely to convert more than 40% of the available wind energy. Different solutions are proposed to minimize WT energy loss and improve performance, such as the use of speed increasers, counter-rotating wind rotors or counter-rotating electric generators. Downsizing the design, saving weight and reducing the cost of WT conversion systems, while increasing their efficiency, have posed constant challenges to WT designers. Nevertheless, very little research in the field is concerned with, and partially recommends, the design of conversion systems. Therefore, the aim of this paper is to propose a specific algorithm for the conceptual synthesis of speed increasers integrated in WT conversion systems, starting with an inventory of all combinations of the main components of a conversion system that prove compatible for efficient functioning. The algorithm is structured in two sections: the first one includes a four-step approach to WT system design, while the second one follows a three-step procedure for identifying the speed increaser concept. Twenty-two variants of speed increasers are further generated and analyzed, four of which are innovative solutions proposed by the authors. The paper also provides guidelines for identifying the WT conversion system concept according to the circumstances of its application.
Study on the Appraisal of Tourism Demands and Recreation Benefits for Nanwan Beach, Kenting, Taiwan
Authors: Dong Chih-Ming , Lin Chien-Chi , Lin Shu-Ping
Published: August 2018

This study implemented a questionnaire survey on tourists to the Nanwan Beach, Kenting, Taiwan and then applied the travel cost method to appraise the recreation benefits of the Nanwan Beach. The truncated Poisson model (TPOIS), truncated negative binomial distribution model, and on-site Poisson model were applied in view of the errors caused by truncated samples and endogenous stratification, and the results indicated that: (1) The on-site Poisson model was more suitable than the other two models for estimating the recreation benefits of Nanwan; (2) the three recreational benefit indicators (consumer surplus, compensation variation, and equivalent variation) estimated using the TPOIS model were all significantly greater than those of the on-site Poisson model; (3) the on-site Poisson model estimated the price elasticity and income elasticity of the tourism demands for Nanwan as −0.329 and 0.187, respectively; and (4) on the basis of the on-site Poisson model, the consumer surplus for Nanwan was NT$9639 (approximately US$289) per person per visit, and the annual gross recreation benefits were approximate NT$8.022 billion. The results are expected to provide a valuable reference for management and planning policies of the Kenting National Park.
Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus
Authors: Zhu Jialei , Tian Ju , Wang Jun , Nie Shuijing
Published: August 2018

Poplar is an important research organism, and species in sections Tacamahaca and Aigeiros, have advantages in terms of stress resistance, ease of propagation, and fast growth. Poplar species are widely distributed and well-adapted in the world, presenting a large potential for genetic improvement. Hybridization between different species allows us to generate offspring with a unique combinations of traits. This approach has a huge potential for breeding new poplar varieties that could aid in controlling desertification in the arid and semi-arid zones of the “Three-North” in China. In this study, we carried out a cross test scheme with nine female and thirteen male poplar trees. A total of 105,401 seeds were collected from 117 crosses. Flowering phenology and seed maturation differences of the hybrid progeny were monitored in greenhouses. For male trees, Populus deltoides had the longest flowering time. For female trees, Populus pseudo-simonii showed the longest seed maturity time. The number of carpals and ovules were not the same in different females. Meanwhile, three carpals were found in P. pseudo-simonii. A highly significant positive correlation was found between the seed size and the Thousand Kernel Weight, as well as the seedling cotyledon length. During seed germination, non-radicle and non-hypocotyl seedlings were observed. We also observed a number of cotyledon variants, including single and fused cotyledons, two cotyledons with one cotyledon cracking into two parts, three cotyledons, as well as four cotyledons. These results lay a favorable foundation for combining the research between the sections Tacamahaca and Aigeiros in future work.
Fine Root Dynamics in Three Forest Types with Different Origins in a Subalpine Region of the Eastern Qinghai-Tibetan Plateau
Authors: Liu Shun , Luo Da , Yang Hongguo , Shi Zuomin , Liu Qianli , Zhang Li , Kang Ying
Published: August 2018

Fine roots play a crucial role in plant survival potential and biogeochemical cycles of forest ecosystems. Subalpine areas of the Eastern Qinghai-Tibetan Plateau have experienced different forest re-establishment methods after clear-cutting primary forest. However, little is known about fine root dynamics of these forests originating from artificial, natural and their combined processes. Here, we determined fine root traits (biomass, production and turnover rate) of three subalpine forest types, i.e., Picea asperata Mast. plantation forest (artificial planting, PF), natural secondary forest (natural without assisted regeneration, NF) and P. asperata broadleaved mixed forest (natural regeneration after artificial planting, MF) composed of planted P. asperata and naturally regenerated native broadleaved species. At the soil depth of 0–30 cm, fine root biomass was the highest in PF and fine root production was the highest in NF, and both were the lowest in MF. Fine root dynamics of the three forest types tended to decrease with soil depth, with larger variations in PF. Fine root biomass and production were the highest in PF in 0–10 cm soil layer but were not significantly different among forest types in the lower soil layers. There were positive correlations between these parameters and aboveground biomass across forest types in soil layer of 0–10 cm, but not in the lower soil layers. Fine root turnover rate was generally higher in mixed forests than in monocultures at all soil depths. In conclusion, the natural regeneration procedure after clear-cutting in the subalpine region of western Sichuan seems to be superior from the perspective of fine root dynamics.
It’s in the Water: Byzantine Borderlands and the Village War
Authors: Moralee Jason
Published: August 2018

This essay examines Byzantine military manuals created between the sixth to the tenth centuries for what they can reveal about Byzantine imperial attitudes toward the landscapes of war and those who inhabit them. Of foremost concern in these sources is the maintenance of ‘security’ (Greek: asphaleia) by commanders with the necessary quality of ‘experience’ (Greek: peira). Experience meant knowing how to best exploit the land, including the villages under Byzantine authority, in the prosecution of war. Exploitation in the name of security involved destroying villages, using villages and their inhabitants in ambushes, poisoning and seizing crops, evacuating villages, and using villages for the billeting of, at times undisciplined, soldiers. Villages were thus central to a Byzantine military strategy that is identified here as the ‘village war,’ a strategy that is analogous to security strategies evident in more recent conflicts. Through the juxtaposition of premodern and modern modalities of war, this essay intends to be a pointed reminder that the village war has deep roots in imperialist thought, and that the consequences of the village war profoundly reshape the lives of those caught up in its midst, particularly the peasantry.
Risk Assessment and Source Identification of Toxic Metals in the Agricultural Soil around a Pb/Zn Mining and Smelting Area in Southwest China
Authors: Wu Jinnan , Long Jian , Liu Lingfei , Li Juan , Liao Hongkai , Zhang Mingjiang , Zhao Chang , Wu Qiusheng
Published: August 2018

Mining and smelting activities are the primary sources of toxic metal pollution in China. The purpose of this study was to investigate the pollution risk and identify sources of metals in the arable soil of a Zn/Pb mining and smelting district located in Huize, in Southwest China. Topsoil (346) and profile (three) samples were collected and analyzed to determine the total concentrations of eight toxic elements (Cd, Hg, As, Pb, Cr, Cu, Zn and Ni). The results showed that the mean Cd, Hg, As, Pb, Cr, Cu, Zn and Ni concentrations were 9.07, 0.37, 25.0, 512, 88.7, 239, 1761 and 90.3 mg/kg, respectively, all of which exceeded both the Huize and Yunnan soil background levels. Overall the topsoil was quite acidic, with a mean pH of 5.51. The mean geoaccumulation index (Igeo) revealed that the pollution level was in the order of Pb > Zn > Cd > Hg > As > Ni > Cu > Cr. The ecological risk index (Ei) indicated that there were serious contamination risks for Cd and Hg, high risk for Pb, moderate risk for As, and Cd and Hg were the dominant contributors to the high combined ecological risk index (Er) with a mean parameter of 699 meaning a serious ecological risk. The Nemerow pollution index (Pn) showed that 99.1% of soil samples were highly polluted or worse. Horizontally, high concentrations of Cd, Hg, As, Pb and Zn appeared in the north and middle of the study area, while Cr, Cu and Ni showed an opposite trend. Vertically, as the depth increased, Cd, Hg, As, Pb and Zn contents declined, but Cr, Cu and Ni exhibited an increasing trend. The mobilities of the metals were in the order of Zn > Cd > Hg > As > Pb. Horizontal and vertical distribution, coupled with correlation analysis, PCA and CA suggested that Cd, Hg, As, Pb and Zn mainly came from the anthropogenic sources, whereas Cr and Ni had a lithogenic origin. The source of Cu was a combination of the presence of parent materials as well as human activities. This study provides a base for the local government to control the toxic metal pollution and restore the soil environment system and an effective method to identify the sources of the studied pollutants.
Spatially Explicit Age Segregation Index and Self-Rated Health of Older Adults in US Cities
Authors: Deng Guangran , Mao Liang
Published: August 2018

There have been mixed findings on whether residential (spatial) age segregation causes better or worse health in older adults. These inconsistencies can possibly be attributed to two limitations in the previous studies. First, many studies have used statistical age composition to indicate the residential age segregation in a community, but this statistic does not consider the spatial arrangement of the residents. Second, many national scale studies have focused on averaged (or global) associations between age segregation and senior health and have assumed that these associations represent the situation in every part of a country. Little attention has been paid to local patterns of such association in different places. To address these previously identified limitations, we calculated a spatially explicit age segregation index for each United States (US) city to replace the conventional age composition index. We derived data regarding 92,560 respondents aged 65 and above in 185 US urban areas from the Behavioral Risk Factor Surveillance System (BRFSS). We then examined global and local associations between spatial age segregation and the self-rated health of older adults across US cities. Our multilevel global analysis suggested that older adults living in age-segregated metropolitan areas experienced more mentally unhealthy days. On the other hand, the local regression analysis identified local clusters of positive associations between the age segregation and the elderly’s overall health status in western and southern metropolitan areas, but no significant associations in midwestern and northeastern cities. In short, we advocated for the use of a spatially explicit approach to deepen the understanding of the association between age segregation and senior health. The new age segregation metric and new analytic approach can offer new insights into the ongoing debate regarding aging in place.
Exploring the Factors Driving Changes in Farmland within the Tumen/Tuman River Basin
Authors: Kang Cholhyok , Zhang Yili , Paudel Basanta , Liu Linshan , Wang Zhaofeng , Li Ryongsu
Published: August 2018

Understanding farmland changes and their mechanisms is important for food security and sustainable development. This study assesses the farmland changes and their drivers within the Tumen River of China and the Tuman River within the Democratic People’s Republic of Korea (DPR Korea) from 1991 to 2016 (1991–2000, 2000–2010, and 2010–2016). Farmland surfaces in Tumen/Tuman River Basin (TRB) for each of the years were mapped from satellite imagery using an object-based image segmentation and a support vector machine (SVM) approach. A logistic regression was applied to discern the mechanisms underlying farmland changes. Results indicate that cultivated surfaces changes within the two regions were characterized by large differences during the three time periods. The decreases of cultivated surface of −15.55 km2 (i.e., 0.55% of total cultivated surface area in 2000) and −23.61 km2 (i.e., 0.83% of total cultivated surface area in 2016) occurred in China between 1991 and 2000 and between 2010 and 2016, respectively; while an increase of 30.98 km2 (i.e., 1.09% of total cultivated surface area in 2010) was seen between 2000 and 2010. Cultivated surfaces increased within DPR Korea side over the three time periods; a marked increase, in particular, was seen between 1991 and 2000 by 443.93 km2 (i.e., 23.43% of total cultivated surface area in 2000), while farmland increased by 140.87 km2 (i.e., 6.92% of total cultivated surface area in 2010) and 180.86 km2 (i.e., 1.78% of total cultivated surface area in 2016), respectively, between 2000 and 2010 and between 2010 and 2016. We also found that expansions and contractions in farmland within both regions of the TRB were mainly influenced by topographic, soil, climatic, and distance factors, which had different importance degrees. Among these significant forces, the temperatures in the two regions were paramount positive factors on farmland changes during 1991–2016 and slope in China and precipitation in DPR Korea were the paramount negative factors affecting farmland changes, respectively. Additionally, except for between 2000 and 2010 in DPR Korea TRB region, most of the factors significantly influencing the farmland changes revealed the same positive or negative effects in different periods, because of mountainous topography. This study allows enhancing understanding of the mechanisms underlying farmland changes in the TRB.
On the Risk Assessment of Terrorist Attacks Coupled with Multi-Source Factors
Authors: Zhang Xun , Jin Min , Fu Jingying , Hao Mengmeng , Yu Chongchong , Xie Xiaolan
Published: August 2018

Terrorism has wreaked havoc on today’s society and people. The discovery of the regularity of terrorist attacks is of great significance to the global counterterrorism strategy. In this study, we improve the traditional location recommendation algorithm coupled with multi-source factors and spatial characteristics. We used the data of terrorist attacks in Southeast Asia from 1970 to 2016, and comprehensively considered 17 influencing factors, including socioeconomic and natural resource factors. The improved recommendation algorithm is used to build a spatial risk assessment model of terrorist attacks, and the effectiveness is tested. The model trained in this study is tested with precision, recall, and F-Measure. The results show that, when the threshold is 0.4, the precision is as high as 88%, and the F-Measure is the highest. We assess the spatial risk of the terrorist attacks in Southeast Asia through experiments. It can be seen that the southernmost part of the Indochina peninsula and the Philippines are high-risk areas and that the medium-risk and high-risk areas are mainly distributed in the coastal areas. Therefore, future anti-terrorism measures should pay more attention to these areas.
Aberrant Regulation of mRNA m6A Modification in Cancer Development
Authors: Luo Junyun , Liu Hui , Luan Siyu , He Chongsheng , Li Zhaoyong
Published: August 2018

N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNAs (mRNAs). The m6A modification in RNA can be catalyzed by methyltransferases, or removed by demethylases, which are termed m6A writers and erasers, respectively. Selective recognition and binding by distinct m6A reader proteins lead mRNA to divergent destinies. m6A has been reported to influence almost every stage of mRNA metabolism and to regulate multiple biological processes. Accumulating evidence strongly supports the correlation between aberrant cellular m6A level and cancer. We summarize here that deregulation of m6A modification, resulting from aberrant expression or function of m6A writers, erasers, readers or some other protein factors, is associated with carcinogenesis and cancer progression. Understanding the regulation and functional mechanism of mRNA m6A modification in cancer development may help in developing novel and efficient strategies for the diagnosis, prognosis and treatment of human cancers.
Transgenic Silkworm-Based Silk Gland Bioreactor for Large Scale Production of Bioactive Human Platelet-Derived Growth Factor (PDGF-BB) in Silk Cocoons
Authors: Chen Wenjing , Wang Feng , Tian Chi , Wang Yuancheng , Xu Sheng , Wang Riyuan , Hou Kai , Zhao Ping , Yu Ling , Lu Zhisong , Xia Qingyou
Published: August 2018

Human platelet derived growth factor (PDGF) is a major therapeutic protein with great demand in the clinical setting; however, its rate of supply is far from meeting needs. Here, we provide an effective strategy to produce PDGF-BB in large quantities using a transgenic silkworm. The codon-optimized PDGF-B gene regulated by the highly efficient sericin-1 expression system was integrated into the genome of a silkworm. The high transcriptional expression of the PDGF-BB gene in the transgenic silkworm competitively inhibited the transcription expression of the endogenous sericin-1 gene which caused a significant 37.5% decline. The PDGF-BB synthesized in the middle silk gland (MSG) of transgenic silkworms could form a homodimer through intermolecular disulfide bonds, which is then secreted into sericin lumen and finally, distributed in the sericin layer of the cocoon. In this study, a protein quantity of approximately 0.33 mg/g was found in the cocoon. Following a purification process, approximately 150.7 μg of recombinant PDGF-BB with a purity of 82% was purified from 1 g of cocoons. Furthermore, the bioactivity assays showed that the purified recombinant PDGF-BB was able to promote the growth, proliferation and migration of NIH/3T3 cells significantly. These results suggest that the silk gland bioreactor can produce active recombinant PDGF-BB as an efficient mitogen and wound healing agent.
A Cell Junctional Protein Network Associated with Connexin-26
Authors: Batissoco Ana C. , Salazar-Silva Rodrigo , Oiticica Jeanne , Bento Ricardo F. , Mingroni-Netto Regina C. , Haddad Luciana A.
Published: August 2018

GJB2 mutations are the leading cause of non-syndromic inherited hearing loss. GJB2 encodes connexin-26 (CX26), which is a connexin (CX) family protein expressed in cochlea, skin, liver, and brain, displaying short cytoplasmic N-termini and C-termini. We searched for CX26 C-terminus binding partners by affinity capture and identified 12 unique proteins associated with cell junctions or cytoskeleton (CGN, DAAM1, FLNB, GAPDH, HOMER2, MAP7, MAPRE2 (EB2), JUP, PTK2B, RAI14, TJP1, and VCL) by using mass spectrometry. We show that, similar to other CX family members, CX26 co-fractionates with TJP1, VCL, and EB2 (EB1 paralogue) as well as the membrane-associated protein ASS1. The adaptor protein CGN (cingulin) co-immuno-precipitates with CX26, ASS1, and TJP1. In addition, CGN co-immunoprecipitation with CX30, CX31, and CX43 indicates that CX association is independent on the CX C-terminus length or sequence. CX26, CGN, FLNB, and DAMM1 were shown to distribute to the organ of Corti and hepatocyte plasma membrane. In the mouse liver, CX26 and TJP1 co-localized at the plasma membrane. In conclusion, CX26 associates with components of other membrane junctions that integrate with the cytoskeleton.
Signalling Alterations in Bones of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Gene Deficient Mice
Authors: Józsa Gergő , Szegeczki Vince , Pálfi Andrea , Kiss Tamás , Helyes Zsuzsanna , Fülöp Balázs , Cserháti Csaba , Daróczi Lajos , Tamás Andrea , Zákány Róza , Reglődi Dóra , Juhász Tamás
Published: August 2018

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with diverse developmental roles, including differentiation of skeletal elements. It is a positive regulatory factor of chondrogenesis and osteogenic differentiation in vitro, but little is known about its in vivo role in bone formation. In our experiments, diaphyses of long bones from hind limbs of PACAP gene-deficient mice showed changes in thickness and increased staining intensity. Our main goal was to perform a detailed morphological and molecular biological analysis of femurs from PACAP knockout (KO) and wild type (WT) mice. Transverse diameter and anterior cortical bone thickness of KO femurs showed significant alterations with disturbed Ca2+ accumulation and collagen type I expression. Higher expression and activity of alkaline phosphatase were also observed, accompanied by increased fragility PACAP KO femurs. Increased expression of the elements of bone morphogenic protein (BMP) and hedgehog signalling was also observed, and are possibly responsible for the compensation mechanism accounting for the slight morphological changes. In summary, our results show that lack of PACAP influences molecular and biomechanical properties of bone matrix, activating various signalling cascade changes in a compensatory fashion. The increased fragility of PACAP KO femur further supports the role of endogenous PACAP in in vivo bone formation.
Individual Security and Network Design with Malicious Nodes
Authors: Janus Tomasz , Skomra Mateusz , Dziubiński Marcin
Published: August 2018

Networks are beneficial to those being connected but can also be used as carriers of contagious hostile attacks. These attacks are often facilitated by exploiting corrupt network users. To protect against the attacks, users can resort to costly defense. The decentralized nature of such protection is known to be inefficient, but the inefficiencies can be mitigated by a careful network design. Is network design still effective when not all users can be trusted? We propose a model of network design and defense with byzantine nodes to address this question. We study the optimal defended networks in the case of centralized defense and, for the case of decentralized defense, we show that the inefficiencies due to decentralization can be mitigated arbitrarily well when the number of nodes in the network is sufficiently large, despite the presence of the byzantine nodes.
Pathway Analysis of a Transcriptome and Metabolite Profile to Elucidate a Compensatory Mechanism for Taurine Deficiency in the Heart of Taurine Transporter Knockout Mice
Authors: Ito Takashi , Murakami Shigeru , Schaffer Stephen
Published: August 2018

Taurine, which is abundant in mammalian tissues, especially in the heart, is essential for cellular osmoregulation. We previously reported that taurine deficiency leads to changes in the levels of several metabolites, suggesting that alterations in those metabolites might compensate in part for tissue taurine loss, a process that would be important in maintaining cardiac homeostasis. In this study, we investigated the molecular basis for changes in the metabolite profile of a taurine-deficient heart using pathway analysis based on the transcriptome and metabolome profile in the hearts of taurine transporter knockout mice (TauTKO mice), which have been reported by us. First, the genes associated with transport activity, such as the solute carrier (SLC) family, are increased in TauTKO mice, while the established transporters for metabolites that are elevated in the TauTKO heart, such as betaine and carnitine, are not altered by taurine deficiency. Second, the integrated analysis using transcriptome and metabolome data revealed significant increases and/or decreases in the genes involved in Arginine metabolism, Ketone body degradation, Glycerophospholipid metabolism, and Fatty acid metabolism in the KEGG pathway database. In conclusion, these pathway analyses revealed genetic compensatory mechanisms involved in the control of the metabolome profile of the taurine-deficient heart.
An Artificial Neural Network Model for Predicting Successful Extubation in Intensive Care Units
Authors: Hsieh Meng-Hsuen , Hsieh Meng-Ju , Chen Chin-Ming , Hsieh Chia-Chang , Chao Chien-Ming , Lai Chih-Cheng
Published: August 2018

Background: Successful weaning from mechanical ventilation is important for patients in intensive care units (ICUs). The aim was to construct neural networks to predict successful extubation in ventilated patients in ICUs. Methods: Data from 1 December 2009 through 31 December 2011 of 3602 patients with planned extubation in Chi-Mei Medical Center’s ICUs was used to train and test an artificial neural network (ANN). The input was 37 clinical risk factors, and the output was a failed extubation prediction. Results: One hundred eighty-five patients (5.1%) had a failed extubation. Multivariate analyses revealed that failure was positively associated with therapeutic intervention scoring system (TISS) scores (odds ratio [OR]: 1.814; 95% Confidence Interval [CI]: 1.283–2.563), chronic hemodialysis (OR: 12.264; 95% CI: 8.556–17.580), rapid shallow breathing (RSI) (OR: 2.003; 95% CI: 1.378–2.910), and pre-extubation heart rate (OR: 1.705; 95% CI: 1.173–2.480), but negatively associated with pre-extubation PaO2/FiO2 (OR: 0.529; 95%: 0.370–0.750) and maximum expiratory pressure (MEP) (OR: 0.610; 95% CI: 0.413–0.899). A multilayer perceptron ANN model with 19 neurons in a hidden layer was developed. The overall performance of this model was F1: 0.867, precision: 0.939, and recall: 0.822. The area under the receiver operating characteristic curve (AUC) was 0.85, which is better than any one of the following predictors: TISS: 0.58 (95% CI: 0.54–0.62; p < 0.001); 0.58 (95% CI: 0.53–0.62; p < 0.001); and RSI: 0.54 (95% CI: 0.49–0.58; p = 0.097). Conclusions: The ANN performed well when predicting failed extubation, and it will help predict successful planned extubation.
Developing Forest Cover Composites through a Combination of Landsat-8 Optical and Sentinel-1 SAR Data for the Visualization and Extraction of Forested Areas
Authors: Sharma Ram C. , Hara Keitarou , Tateishi Ryutaro
Published: August 2018

Mapping the distribution of forested areas and monitoring their spatio-temporal changes are necessary for the conservation and management of forests. This paper presents two new image composites for the visualization and extraction of forest cover. By exploiting the Landsat-8 satellite-based multi-temporal and multi-spectral reflectance datasets, the Forest Cover Composite (FCC) was designed in this research. The FCC is an RGB (red, green, blue) color composite made up of short-wave infrared reflectance and green reflectance, specially selected from the day when the Normalized Difference Vegetation Index (NDVI) is at a maximum, as the red and blue bands, respectively. The annual mean NDVI values are used as the green band. The FCC is designed in such a way that the forested areas appear greener than other vegetation types, such as grasses and shrubs. On the other hand, the croplands and barren lands are usually seen as red and water/snow is seen as blue. However, forests may not necessarily be greener than other perennial vegetation. To cope with this problem, an Enhanced Forest Cover Composite (EFCC) was designed by combining the annual median backscattering intensity of the VH (vertical transmit, horizontal receive) polarization data from the Sentinel-1 satellite with the green term of the FCC to suppress the green component (mean NDVI values) of the FCC over the non-forested vegetative areas. The performances of the FCC and EFCC were evaluated for the discrimination and classification of forested areas all over Japan with the support of reference data. The FCC and EFCC provided promising results, and the high-resolution forest map newly produced in the research provided better accuracy than the extant MODIS (Moderate Resolution Imaging Spectroradiometer) Land Cover Type product (MCD12Q1) in Japan. The composite images proposed in the research are expected to improve forest monitoring activities in other regions as well.
Protective Effect of Eckol against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice
Authors: Li Shulan , Liu Juan , Zhang Mengya , Chen Yuan , Zhu Tianxing , Wang Jun
Published: August 2018

Several in vitro studies have shown the potential hepatoprotective properties of eckol, a natural phlorotannin derived from the brown alga. However, the in vivo hepatoprotective potential of eckol has not been determined. In this study, we performed an in vivo study to investigate the protective effect of eckol and its possible mechanisms on the carbon tetrachloride (CCl4)-induced acute liver injury model in mice. Results revealed that eckol pre-treatment at the dose of 0.5 and 1.0 mg/kg/day for 7 days significantly suppressed the CCl4-induced increases of alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum and meliorated morphological liver injury. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) analysis showed that the number of positive apoptotic hepatocytes in the eckol-treated group was lower than that in the CCl4 model group. Western blotting analysis also demonstrated the enhanced expression of bcl-2 and suppressed expression of cleaved caspase-3 by eckol. The CCl4-induced oxidative stress in liver was significantly ameliorated by eckol, which was characterized by reduced malondialdehyde (MDA) formations, and enhanced superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and glutathione (GSH) content. Moreover, the CCl4-induced elevations of pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were markedly suppressed in the eckol-treated group. However, eckol enhanced the level of IL-10, a potent anti-inflammatory cytokine, and recruited CD11c+ dendritic cells into the liver tissues of CCl4-treated mice. These results indicated that eckol has the protective effect on CCl4-induced acute liver injury via multiple mechanisms including anti-apoptosis, anti-oxidation, anti-inflammation and immune regulation.
Modification of Nanofiber Support Layer for Thin Film Composite Forward Osmosis Membranes via Layer-by-Layer Polyelectrolyte Deposition
Authors: Gonzales Ralph , Park Myoung , Tijing Leonard , Han Dong , Phuntsho Sherub , Shon Ho
Published: August 2018

Electrospun nanofiber-supported thin film composite membranes are among the most promising membranes for seawater desalination via forward osmosis. In this study, a high-performance electrospun polyvinylidenefluoride (PVDF) nanofiber-supported thin film composite (TFC) membrane was successfully fabricated after molecular layer-by-layer polyelectrolyte deposition. Negatively-charged electrospun polyacrylic acid (PAA) nanofibers were deposited on electrospun PVDF nanofibers to form a support layer consisted of PVDF and PAA nanofibers. This resulted to a more hydrophilic support compared to the plain PVDF nanofiber support. The PVDF-PAA nanofiber support then underwent a layer-by-layer deposition of polyethylenimine (PEI) and PAA to form a polyelectrolyte layer on the nanofiber surface prior to interfacial polymerization, which forms the selective polyamide layer of TFC membranes. The resultant PVDF-LbL TFC membrane exhibited enhanced hydrophilicity and porosity, without sacrificing mechanical strength. As a result, it showed high pure water permeability and low structural parameter values of 4.12 L m−2 h−1 bar−1 and 221 µm, respectively, significantly better compared to commercial FO membrane. Layer-by-layer deposition of polyelectrolyte is therefore a useful and practical modification method for fabrication of high performance nanofiber-supported TFC membrane.
Electrodeposition Kinetics of Ni/Nano-Y2O3 Composite Coatings
Authors: Zhou Xinyu , Wang Yiyong , Liu Xianglin , Liang Zhipeng , Jin Hui
Published: August 2018

Ni/nano-Y2O3 composite films were successfully prepared by electrochemical deposition using an acid sulfamate bath. The influence of solid particles added to electrolyte on electrodeposition was investigated by electrochemical measurement methods. The linear sweep voltammetry test showed that the composite deposition took place at a greater potential than that of nickel, and the presence of nano-Y2O3 decreased cathodic polarization. Chronoamperometry studies indicated that the nucleation model of both deposits similarly approached the theoretical instantaneous nucleation mode based on the Scharifker–Hills model. The Y2O3 particles adsorbed on the cathodic surface were shown to facilitate the nucleation/growth of the nickel matrix which is consistent with the deposition kinetics parameters calculated by non-linear fitting experimental curves. The results of electrochemical impedance spectroscopy showed that the presence of Y2O3 particles in a bath is beneficial for the decrease in charge transfer resistance in the deposition. The atomic force microscopy observations of both deposits obtained in the initial electrodeposition stage confirmed that the Ni-Y2O3 composite had a higher grain number and finer mean grain size.
Additive Manufactured A357.0 Samples Using the Laser Powder Bed Fusion Technique: Shear and Tensile Performance
Authors: Denti Lucia
Published: August 2018

New aluminium alloys, with lower silicon content than in the first-developed formulations, have recently been introduced in the field of Additive Manufacturing and are dedicated to automotive applications. As they are relatively new, mechanical characterization under standard protocols of the automotive field are of utmost scientific as well as industrial relevance. The paper addresses the mechanical properties and microstructure of A357.0. Static tensile and shear tests of samples built by Laser Powder Bed Fusion, with different orientations in the machine work volume, have been performed. The aim was to identify possible anisotropy in the tensile and shear behaviour of this innovative alloy. Particularly for shear, the effect of adhesion between the layers onto shear strength was studied. Results analysis, by means of statistical tools, allows for the affirmation that no tensile modulus or yield strength anisotropy is observed. Instead, a small (yet statistically significant) increase in both shear- and tensile strength and a decrease in ductility are obtained as the direction of the specimens approaches the growth direction. Scanning Electron Microscope (SEM) observation of the failure mechanisms assisted in the interpretation of the results, by relating different failure modes to the relative orientation of loads versus the directions of inherent anisotropy in Laser Powder Bed Fusion processes.