bluuebunny/crossref_metadata_2025 huggingface dataset:
New Modulation Technique to Mitigate Common Mode Voltage Effects in Star-Connected Five-Phase AC Drives
Authors: Fernandez Markel , Sierra-Gonzalez Andres , Robles Endika , Kortabarria Iñigo , Ibarra Edorta , Martin Jose Luis
Published: January 2020

Star-connected multiphase AC drives are being considered for electromovility applications such as electromechanical actuators (EMA), where high power density and fault tolerance is demanded. As for three-phase systems, common-mode voltage (CMV) is an issue for multiphase drives. CMV leads to shaft voltages between rotor and stator windings, generating bearing currents which accelerate bearing degradation and produce high electromagnetic interferences (EMI). CMV effects can be mitigated by using appropriate modulation techniques. Thus, this work proposes a new Hybrid PWM algorithm that effectively reduces CMV in five-phase AC electric drives, improving their reliability. All the mathematical background required to understand the proposal, i.e., vector transformations, vector sequences and calculation of analytical expressions for duty cycle determination are detailed. Additionally, practical details that simplify the implementation of the proposal in an FPGA are also included. This technique, HAZSL5M5-PWM, extends the linear range of the AZSL5M5-PWM modulation, providing a full linear range. Simulation results obtained in an accurate multiphase EMA model are provided, showing the validity of the proposed modulation approach.
National Deadlock. Hot Nationalism, Dual Identities and Catalan Independence (2008–2019)
Authors: Quiroga Alejandro , Molina Fernando
Published: January 2020

The article explores the transformations of Spanish and Catalan national identities and the growth of the pro-independence movement in Catalonia following the 2008 global recession. It argues that the Great Recession provided a new historical context of hot nationalism in which Catalanist narratives of loss and resistance began to ring true to large sectors of Catalan society, whereas the Spanish constitutionalist narratives seemed increasingly outdated. The article also shows the limits of the process of mass nationalization by both the Catalan and the Spanish governments and the eventual ‘crystallization’ of an identity and political divide between pro and anti-independence supporters which split Catalan society down the middle and led to a sort of national identity deadlock.
Human Activity and Hydrogeochemical Processes Relating to Groundwater Quality Degradation in the Yuncheng Basin, Northern China
Authors: Gao Xubo , Li Xue , Wang Wanzhou , Li Chengcheng
Published: January 2020

Groundwater quality degradation has raised widespread concerns about water supplies and ecological crises in China. In this study, hydrogeochemistry, environmental stable isotopes (δ18O, δD), and principal component analysis were conducted together to reveal the mechanism’s response to the hydrogeochemical and quality degradation of groundwater in Yuncheng Basin, Northern China, so that reasonable water resource management strategies can be developed. The study reveals that groundwater faces a tremendous risk of quality decrease during the past decade: (1) the hydrochemical facies of groundwater shows that the bicarbonate and chloride type water was replaced with sulfate type water and the occupying area of SO4·Cl-Na, SO4·HCO3-Na type water expanded dramatically in shallow and intermediate-deep aquifers. (2) Major ion chemistry and hydrogen and oxygen isotope compositions indicate that the major hydrogeochemical processes responsible for groundwater quality deterioration include the dissolution of evaporates (i.e., halite, gypsum, and mirabilite), ion exchange, and evaporation process. Additionally, (3) anthropogenic activities (overutilization of fertilizer) have resulted in nitrate contamination, and have thereby led to groundwater quality degradation.
Effects of Tabata Training During Physical Education Classes on Body Composition, Aerobic Capacity, and Anaerobic Performance of Under-, Normal- and Overweight Adolescents
Authors: Domaradzki Jarosław , Cichy Ireneusz , Rokita Andrzej , Popowczak Marek
Published: January 2020

Physical education classes often fail to include sufficient exercise intensity to induce changes in body tissue composition and physical fitness. Short-term high-intensity interval training protocols incorporated into physical education lessons are one possible solution to this problem. Existing studies have not examined how individuals differing in body mass index (e.g., normal-weight, underweight) respond to high-intensity interval training exercises. Therefore, this study aimed to evaluate the effects of a Tabata protocol on body composition measurements, aerobic capacity, and motor performance in underweight and overweight adolescents (the experimental groups) vs normal-weight adolescents (here regarded as the control group). The sample included 58 adolescents (28 boys, mean age = 16.2 years; 30 girls, mean age = 16.2 years) who completed the high-intensity interval training and the following set of measurements pre- and post- intervention: height, weight, body fat percentage and waist-to-hip ratio, physical efficiency index (based on the Harvard Step Test), agility (in 4 × 10 shuttle run test), and lower-limb muscle power in vertical jump. The results showed that high-intensity interval training was effective in reducing body weight, waist-to-hip ratio, and body fat percentage only in overweight individuals. Improvement in aerobic capacity was found only in underweight and overweight boys. Further research should focus on the development of customized exercise protocols and their adaptation to girls and assess the sustainability of the changes induced.
Dog and Cat Ownership Predicts Adolescents’ Mental Well-Being: A Population-Based Longitudinal Study
Authors: Endo Kaori , Yamasaki Syudo , Ando Shuntaro , Kikusui Takefumi , Mogi Kazutaka , Nagasawa Miho , Kamimura Itsuka , Ishihara Junko , Nakanishi Miharu , Usami Satoshi , Hiraiwa-Hasegawa Mariko , Kasai Kiyoto , Nishida Atsushi
Published: January 2020

A potential association between pet ownership and mental well-being is suggested, but there is a shortage of high-quality longitudinal studies that consider probable differences among different species. We aimed to examine whether ownership of the most popular pets (dogs and cats) would predict mental well-being. The Tokyo Teen Cohort (TTC), a prospective population-based birth cohort study, had dog and cat ownership data at age 10 and mental well-being score at ages 10 and 12 from 2584 adolescents. Linear regression analysis with adjusting for covariates showed that dog ownership had a positive effect on mental well-being compared to no dog ownership, however, cat ownership had a negative effect compared to no cat ownership. Two-factor mixed-design analysis of variance showed that dog ownership predicted maintained mental well-being, while cat ownership predicted progressing decline of mental well-being. Thus, dog and cat ownership may have different effects on adolescents’ mental well-being, implying that the underlying mechanisms that are activated by these types of ownership may differ.
High-Mobility Group Box 1 Protein Signaling in Painful Diabetic Neuropathy
Authors: Thakur Vikram , Sadanandan Jayanarayanan , Chattopadhyay Munmun
Published: January 2020

Diabetes is a global epidemic and more than 50% diabetic patients are also diagnosed with neuropathy, which greatly affects the quality of life of the patients. Available treatments are not always successful due to the limited efficacy and complications, such as addiction and dependency. Studies have implicated that high mobility group box1 (HMGB1) protein plays a crucial role in neuroinflammation and the development of neuropathic conditions. HMGB1 is a proinflammatory cytokine that can be released from necrotic cells in passive form or in response to inflammatory signals as an active form. HMGB1 is the ligand for the receptor for advanced glycation end products (RAGE), and toll-like receptors, (TLR)-2 and TLR4, which also indirectly activates C-X-C chemokine receptor type 4 (CXCR4). We investigated whether blocking of HMGB1 can reduce pain and inflammation in diabetic neuropathic animals to further understand the role of HMGB1 in diabetic neuropathy. Type 2 diabetic rats and mice were treated with natural inhibitor of HMGB1, glycyrrhizin (GLC) for five days/week for four weeks at a dose of 50 mg/kg per day by intraperitoneal injection. The animals were divided into three categories: naïve control, diabetic alone, diabetic with GLC treatment. All of the behavioral analyses were conducted before and after the treatment. The expression of inflammatory markers and changes in histone acetylation in the peripheral nervous system were measured by immunohistochemistry and Western blot analysis after the completion of the treatment. Our study revealed that TLR4, HMGB1, CXCR4, and Nod-like receptor protein 3 (NLRP3) levels were increased in the spinal and dorsal root ganglia (DRG) neurons of Type 2 diabetic mice and rats with painful neuropathy. GLC treatment inhibited the increases in TLR4, NLRP3, and CXCR4 expressions and improved the mechanical and thermal pain threshold in these animals. Immunohistochemical studies revealed that hyperglycemia mediated inflammation influenced HMGB1 acetylation and its release from the neurons. It also altered histone 3 acetylation in the microglial cells. The inhibition of HMGB1 by GLC prevented the release of HMGB1 as well as H3K9 acetylation. These findings indicate that the interruption of HMGB1 mediated inflammation could ameliorate diabetic neuropathy and might exhibit a unique target for the treatment.
Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury
Authors: Ferrero-Andrés Ana , Panisello-Roselló Arnau , Serafín Anna , Roselló-Catafau Joan , Folch-Puy Emma
Published: January 2020

Acute pancreatitis is an inflammatory disorder of the pancreas. Its presentation ranges from self-limiting disease to acute necrotizing pancreatitis (ANP) with multiorgan failure and a high mortality. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic, and water-soluble chemicals composed of repeating units of ethylene glycol. The present article explores the effect of PEG35 administration on reducing the severity of ANP and associated lung injury. ANP was induced by injection of 5% sodium taurocholate into the biliopancreatic duct. PEG35 was administered intravenously either prophylactically or therapeutically. Three hours after ANP induction, pancreas and lung tissue samples and blood were collected and ANP severity was assessed. To evaluate the inflammatory response, gene expression of pro-inflammatory cytokines and chemokine and the changes in the presence of myeloperoxidase and adhesion molecule levels were determined in both the pancreas and the lung. To evaluate cell death, lactate dehydrogenase (LDH) activity and apoptotic cleaved caspase-3 localization were determined in plasma and in both the pancreatic and lung tissue respectively. ANP-associated local and systemic inflammatory processes were reduced when PEG35 was administered prophylactically. PEG35 pre-treatment also protected against acute pancreatitis-associated cell death. Notably, the therapeutic administration of PEG35 significantly decreased associated lung injury, even when the pancreatic lesion was equivalent to that in the untreated ANP-induced group. Our results support a protective role of PEG35 against the ANP-associated inflammatory process and identify PEG35 as a promising tool for the treatment of the potentially lethal complications of the disease.
Electrophysiologic Effects of Growth Hormone Post-Myocardial Infarction
Authors: Stamatis Konstantinos V. , Kontonika Marianthi , Daskalopoulos Evangelos P. , Kolettis Theofilos M.
Published: January 2020

Myocardial infarction remains a major health-related problem with significant acute and long-term consequences. Acute coronary occlusion results in marked electrophysiologic alterations that can induce ventricular tachyarrhythmias such as ventricular tachycardia or ventricular fibrillation, often heralding sudden cardiac death. During the infarct-healing stage, hemodynamic and structural changes can lead to left ventricular dilatation and dysfunction, whereas the accompanying fibrosis forms the substrate for re-entrant circuits that can sustain ventricular tachyarrhythmias. A substantial proportion of such patients present clinically with overt heart failure, a common disease-entity associated with high morbidity and mortality. Several lines of evidence point toward a key role of the growth hormone/insulin-like growth factor-1 axis in the pathophysiology of post-infarction structural and electrophysiologic remodeling. Based on this rationale, experimental studies in animal models have demonstrated attenuated dilatation and improved systolic function after growth hormone administration. In addition to ameliorating wall-stress and preserving the peri-infarct myocardium, antiarrhythmic actions were also evident after such treatment, but the precise underlying mechanisms remain poorly understood. The present article summarizes the acute and chronic actions of systemic and local growth hormone administration in the post-infarction setting, placing emphasis on the electrophysiologic effects. Experimental and clinical data are reviewed, and hypotheses on potential mechanisms of action are discussed. Such information may prove useful in formulating new research questions and designing new studies that are expected to increase the translational value of growth hormone therapy after acute myocardial infarction.
Genome-Wide Identification, Expression Profile and Evolution Analysis of Karyopherin β Gene Family in Solanum tuberosum Group Phureja DM1-3 Reveals Its Roles in Abiotic Stresses
Authors: Xu Ya , Liu Lu , Zhao Pan , Tong Jing , Zhong Naiqin , Zhang Hongji , Liu Ning
Published: January 2020

In eukaryotic cells, nucleocytoplasmic trafficking of macromolecules is largely mediated by Karyopherin β/Importin (KPNβ or Impβ) nuclear transport factors, and they import and export cargo proteins or RNAs via the nuclear pores across the nuclear envelope, consequently effecting the cellular signal cascades in response to pathogen attack and environmental cues. Although achievements on understanding the roles of several KPNβs have been obtained from model plant Arabidopsis thaliana, comprehensive analysis of potato KPNβ gene family is yet to be elucidated. In our genome-wide identifications, a total of 13 StKPNβ (Solanum tuberosum KPNβ) genes were found in the genome of the doubled monoploid S. tuberosum Group Phureja DM1-3. Sequence alignment and conserved domain analysis suggested the presence of importin-β N-terminal domain (IBN_N, PF08310) or Exporin1-like domain (XpoI, PF08389) at N-terminus and HEAT motif at the C-terminal portion in most StKPNβs. Phylogenetic analysis indicated that members of StKPNβ could be classified into 16 subgroups in accordance with their homology to human KPNβs, which was also supported by exon-intron structure, consensus motifs, and domain compositions. RNA-Seq analysis and quantitative real-time PCR experiments revealed that, except StKPNβ3d and StKPNβ4, almost all StKPNβs were ubiquitously expressed in all tissues analyzed, whereas transcriptional levels of several StKPNβs were increased upon biotic/abiotic stress or phytohormone treatments, reflecting their potential roles in plant growth, development or stress responses. Furthermore, we demonstrated that silencing of StKPNβ3a, a SA- and H2O2-inducible KPNβ genes led to increased susceptibility to environmental challenges, implying its crucial roles in plant adaption to abiotic stresses. Overall, our results provide molecular insights into StKPNβ gene family, which will serve as a strong foundation for further functional characterization and will facilitate potato breeding programs.
Lack of PKCθ Promotes Regenerative Ability of Muscle Stem Cells in Chronic Muscle Injury
Authors: Fiore Piera Filomena , Benedetti Anna , Sandonà Martina , Madaro Luca , De Bardi Marco , Saccone Valentina , Puri Pier Lorenzo , Gargioli Cesare , Lozanoska-Ochser Biliana , Bouché Marina
Published: January 2020

Duchenne muscular dystrophy (DMD) is a genetic disease characterized by muscle wasting and chronic inflammation, leading to impaired satellite cells (SCs) function and exhaustion of their regenerative capacity. We previously showed that lack of PKCθ in mdx mice, a mouse model of DMD, reduces muscle wasting and inflammation, and improves muscle regeneration and performance at early stages of the disease. In this study, we show that muscle regeneration is boosted, and fibrosis reduced in mdxθ−/− mice, even at advanced stages of the disease. This phenotype was associated with a higher number of Pax7 positive cells in mdxθ−/− muscle compared with mdx muscle, during the progression of the disease. Moreover, the expression level of Pax7 and Notch1, the pivotal regulators of SCs self-renewal, were upregulated in SCs isolated from mdxθ−/− muscle compared with mdx derived SCs. Likewise, the expression of the Notch ligands Delta1 and Jagged1 was higher in mdxθ−/− muscle compared with mdx. The expression level of Delta1 and Jagged1 was also higher in PKCθ−/− muscle compared with WT muscle following acute injury. In addition, lack of PKCθ prolonged the survival and sustained the differentiation of transplanted myogenic progenitors. Overall, our results suggest that lack of PKCθ promotes muscle repair in dystrophic mice, supporting stem cells survival and maintenance through increased Delta-Notch signaling.
Novel Biomarkers of Hepatitis B and Hepatocellular Carcinoma: Clinical Significance of HBcrAg and M2BPGi
Authors: Baudi Ian , Inoue Takako , Tanaka Yasuhito
Published: January 2020

The hepatitis B virus (HBV) cannot be removed completely from infected hepatocytes, owing to the presence of intrahepatic covalently closed circular DNA (cccDNA). As chronic hepatitis B (CHB) can progress to cirrhosis and hepatocellular carcinoma (HCC), predicting HCC development in high-risk patients with high viral replicative activity or advanced fibrosis is important. Novel serological biomarkers reflect intrahepatic viral replicative activity or the progression of liver fibrosis, indicating non-invasive alternatives to liver biopsy: (1) Hepatitis B core-related antigen (HBcrAg) correlates with serum HBV DNA and intrahepatic cccDNA. In CHB patients, a decrease in HBcrAg is associated with favorable outcomes. HBcrAg can predict HCC occurrence or recurrence. (2) Measurement of the Mac-2 binding protein glycosylation isomer (M2BPGi) has been introduced for the evaluation of liver fibrosis. An increase in M2BPGi in CHB patients is related to the progression of liver fibrosis and high potential (risk) of HCC development. Here, we describe the clinical applications of HBcrAg and M2BPGi in CHB patients. Additionally, because new potential therapeutic agents that eliminate intrahepatic cccDNA are being developed, monitoring of HBcrAg or M2BPGi might be suitable for evaluating therapeutic effects and the clinical outcomes. In conclusion, these would be appropriate surrogate markers for predicting disease progression.
ColXV Aggravates Adipocyte Apoptosis by Facilitating Abnormal Extracellular Matrix Remodeling in Mice
Authors: Xia Tianyu , Shen Zhentong , Cai Jiarui , Pan Miao , Sun Chao
Published: January 2020

The extracellular matrix (ECM) is a highly dynamic structural network and plays an essential role in cell behavior and regulation during metabolic homeostasis and obesity progression. Abnormal ECM remodeling impairs adipocyte plasticity required for diverse cellular functions. Collagen XV (ColXV) is a proteoglycan localized to the outermost layer of basement membranes (BMs) and forms a bridge between the BMs and the fibrillar collagen matrix. Nevertheless, how ColXV affects ECM composition and the reason for subsequent adipocyte apoptosis is still unclear. This report found, through RNA-seq data, that ColXV is linked to cell growth and ECM remodeling. Findings show that, in response to excessive expression of extracellular ColXV, the AMPK/mTORC1 pathway is strongly activated and triggers a cascade of mitochondrial apoptosis. This is the first study to make use of ECM three-dimensional reconstruction, based on decellularization in the adipose tissues and the study reveals that ColXV is an activation factor that alters ECM remodeling in adipose tissues. It was also demonstrated that the fibroblast growth factor 2 (FGF2)/fibroblast growth factor receptor 1 (FGFR1) axis involved in ECM remodeling is suppressed by ColXV due to reduction of FGF2 translocation to FGFR1. Furthermore, ColXV induced remodeling of ECM preceding apoptosis and continued to induce apoptosis in adipocytes. Collectively, our findings establish ColXV as a basement membrane collagen with homology to ColXVIII, indicating that it is one of the positive regulators for inducing ECM remodeling and further promoting adipocyte apoptosis.
The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression
Authors: Hansen Marten , Zeddies Sabrina , Meinders Marjolein , di Summa Franca , Rollmann Ewa , van Alphen Floris P.J. , Hoogendijk Arjan J. , Moore Kat S. , Halbach Melanie , Gutiérrez Laura , van den Biggelaar Maartje , Thijssen-Timmer Daphne C. , Auburger Georg W.J. , van den Akker Emile , von Lindern Marieke
Published: January 2020

Megakaryopoiesis is the process during which megakaryoblasts differentiate to polyploid megakaryocytes that can subsequently shed thousands of platelets in the circulation. Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34+/CD41+ megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to hemostasis. Mice deficient for Atxn2 did not display differences in bleeding times, but the expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), was deregulated and platelet aggregation upon specific triggers was reduced.
Acknowledgement to Reviewers of Molbank in 2019
Authors: Molbank Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
Self-Etch Silane Primer: Reactivity and Bonding with a Lithium Disilicate Ceramic
Authors: Dimitriadi Maria , Zinelis Spiros , Zafiropoulou Maria , Silikas Nikolaos , Eliades George
Published: January 2020

The aim of the study was to evaluate the stability, reactivity, and bond strength with a lithium disilicate ceramic of a self-etch silane primer (Monobond Etch and Prime/MEP). The stability was evaluated by 1H-,31P-NMR spectroscopy (before/after aging), and the reactivity by micro MIR-FTIR spectroscopy on Ge surfaces (0, 1, 24 h) using a prehydrolyzed silane primer (Calibra Silane Coupling Agent/CLB), as a control. The effect of MEP vs. 5% HF-etching on ceramic roughness was assessed by optical profilometry. The shear bond strength (SBS) of a resin composite bonded to polished ceramic surfaces treated with MEP, HF without silane (HF+NS), HF+CLB, and HF+MEP (n = 20) was evaluated after storage in water (A: 37 °C/1 week, B: 5000×/5–55 °C and C: 100 °C/24 h). Aging did not affect the silanol groups of MEP, but only the phosphate co-monomer. Silanols were reactive forming siloxanes, but exhibited lower consumption rate than CLB. HF-etching induced significantly higher values than MEP, in all the roughness parameters tested (Sa, Sz, Sdr, Sc, Sv), with the greatest differences found in Sdr and Sv. For SBS, MEP was inferior to all treatments/storage conditions, except of HF+NS in A, where the values were similar. However, on a HF-etched substrate, MEP provided highest strength and reliability.
Xanthones, A Promising Anti-Inflammatory Scaffold: Structure, Activity, and Drug Likeness Analysis
Authors: Feng Zheling , Lu Xiuqiang , Gan Lishe , Zhang Qingwen , Lin Ligen
Published: January 2020

Inflammation is the body’s self-protective response to multiple stimulus, from external harmful substances to internal danger signals released after trauma or cell dysfunction. Many diseases are considered to be related to inflammation, such as cancer, metabolic disorders, aging, and neurodegenerative diseases. Current therapeutic approaches include mainly non-steroidal anti-inflammatory drugs and glucocorticoids, which are generally of limited effectiveness and severe side-effects. Thus, it is urgent to develop novel effective anti-inflammatory therapeutic agents. Xanthones, a unique scaffold with a 9H-Xanthen-9-one core structure, widely exist in natural sources. Till now, over 250 xanthones were isolated and identified in plants from the families Gentianaceae and Hypericaceae. Many xanthones have been disclosed with anti-inflammatory properties on different models, either in vitro or in vivo. Herein, we provide a comprehensive and up-to-date review of xanthones with anti-inflammatory properties, and analyzed their drug likeness, which might be potential therapeutic agents to fight against inflammation-related diseases.
Nickel-Catalyzed Removal of Alkene Protecting Group of Phenols, Alcohols via Chain Walking Process
Authors: Meng Chenkai , Niu Haolin , Ning Juehan , Wu Wengang , Yi Jun
Published: January 2020

An efficient nickel-catalyzed removal of alkene protection group under mild condition with high functional group tolerance through chain walking process has been established. Not only phenolic ethers, but also alcoholic ethers can be tolerated with the retention of stereocenter adjacent to hydroxyl group. The new reaction brings the homoallyl group into a start of new type of protecting group.
Synergistic Antitumor Effects on Drug-Resistant Breast Cancer of Paclitaxel/Lapatinib Composite Nanocrystals
Authors: Wang Jun , Lv Feng-Mei , Wang Dong-Li , Du Jian-Liang , Guo Hai-Yan , Chen Hai-Ni , Zhao Shou-Jin , Liu Zhe-Peng , Liu Yu
Published: January 2020

Drug resistance presents serious difficulties for cancer treatment. A combination of paclitaxel (PTX) and lapatinib (LAPA) shows potentials in multiple drug resistant cancers in the clinic, but it is almost impossible to deliver these two drugs to the tumor at the same time with the best proportion by simple co-administration of the respective current formualtions for their different pharmacokinetic profiles. Here composite nanocrystals of PTX and LAPA (cNC) were designed with a ratio of 2:1 (w/w), which was their intracellular ratio at the best synergistic efficacy on a drug-resistant cancer cell line (MCF-7/ADR). Such cNC were prepared using a bottom-up method to achieve a nearly spherical appearance and a narrow size distribution of 95.1 ± 2.1 nm. For nanocrystal stabilization, Polyethylene glycol (PEG) coating was introduced into the cNC via polydopamine (PDA) coating in order to get a PEGylated composite nanocrystal (cNC@PDA-PEG) with nanoscale size (170.5 ± 1.4 nm), considerable drug loading (PTX: 21.33 ± 1.48%, LAPA: 10.95 ± 1.24%) and good stability for at least 4 days in plasma-containing buffers. Differential scanning calorimeter (DSC) and XRD data both indicated the different crystalline states of the cNC as well as the cNC@PDA-PEG in comparison with bulk drugs. In vitro release data showed that PTX and LAPA were gradually and completely released from cNC@PDA-PEG in 3 days, while drug release from bulk drugs or cNC was only 30%. cNC@PDA-PEG also showed negligible hemolysis in vitro. Cellular uptake experiments in the MCF-7/ADR cell line showed that the nanocrystals entered the cells in a complete form through endocytosis and then released the drug in the cell. cNC@PDA-PEG inhibits the growth of this drug-resistant cell more effectively than the unmodified version (cNC). In summary, PEGylated PTX and LAPA composite nanocrystals showed the potential for treament of drug-resistant tumors by simultaneously delivering two drugs to tumor cells with the best proportion.
Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of Endophytic Fusarium culmorum Strains with Different Effects on Cereals
Authors: Jaroszuk-Ściseł Jolanta , Nowak Artur , Komaniecka Iwona , Choma Adam , Jarosz-Wilkołazka Anna , Osińska-Jaroszuk Monika , Tyśkiewicz Renata , Wiater Adrian , Rogalski Jerzy
Published: January 2020

Exopolymeric substances (EPS) can determine plant-microorganism interactions and have great potential as bioactive compounds. The different amounts of EPS obtained from cultures of three endophytic Fusarium culmorum strains with different aggressiveness—growth promoting (PGPF), deleterious (DRMO), and pathogenic towards cereal plants—depended on growth conditions. The EPS concentrations (under optimized culture conditions) were the lowest (0.2 g/L) in the PGPF, about three times higher in the DRMO, and five times higher in the pathogen culture. The EPS of these strains differed in the content of proteins, phenolic components, total sugars, glycosidic linkages, and sugar composition (glucose, mannose, galactose, and smaller quantities of arabinose, galactosamine, and glucosamine). The pathogen EPS exhibited the highest total sugar and mannose concentration. FTIR analysis confirmed the β configuration of the sugars. The EPS differed in the number and weight of polysaccharidic subfractions. The EPS of PGPF and DRMO had two subfractions and the pathogen EPS exhibited a subfraction with the lowest weight (5 kDa). The three EPS preparations (ethanol-precipitated EP, crude C, and proteolysed P) had antioxidant activity (particularly high for the EP-EPS soluble in high concentrations). The EP-EPS of the PGPF strain had the highest antioxidant activity, most likely associated with the highest content of phenolic compounds in this EPS.
A Quantitative Analysis Model Established to Determine the Concentration of Each Source in Mixed Astaxanthin from Different Sources
Authors: Du Xiping , Wang Xin , Bai Manli , Liu Shaosong , Huang Gaoling , Zhang Qin , Ni Hui , Chen Feng
Published: January 2020

Astaxanthin from different sources possesses different biological activities and optical isomers. The ingredients of astaxanthin mixtures from different sources on the market have often been mislabeled. Therefore, it is important to determine the sources of astaxanthin and their respective concentrations in a mixture. To solve this problem, a quantitative analysis model was established and further verified. The results showed that the deviation between the calculated concentration and the actual concentration ranged from 0 to 7 µg/mL, and the recovery rate was between 88.90% and 103.56%. This indicates that the quantitative analysis model of astaxanthin was feasible and reliable. This study not only has important applications in the astaxanthin mixture component determination but may also shed light on the quantitative analysis of other sample mixtures with stereoisomers from different sources.
Lipidomic Analysis Reveals Specific Differences between Fibroblast and Keratinocyte Ceramide Profile of Patients with Psoriasis Vulgaris
Authors: Łuczaj Wojciech , Wroński Adam , Domingues Pedro , Domingues M Rosário , Skrzydlewska Elżbieta
Published: January 2020

Ceramides are important lipid metabolites for primal skin functions. There is increasing evidence that alteration of the profile and metabolism of ceramides is associated with skin diseases, such as psoriasis vulgaris. Most studies have reported alteration in ceramide content in the stratum corneum, but these have been scarcely reported for other skin layers. In the present work, we aimed to explore changes in the ceramide profile of fibroblasts and keratinocytes in patients with psoriasis vulgaris and healthy subjects. Using the reversed-phase liquid chromatography-quadrupole-time-of-flight-tandem-mass spectrometry (RPLC-QTOF-MS/MS) platform, we identified ceramide containing non-hydroxy fatty acid ([N]), α-hydroxy fatty acid ([A]), and esterified ω-hydroxy fatty acid ([EO]) and 3 sphingoid bases, dihydrosphingosine ([DS]), sphingosine ([S]), and phytosphingosine ([P]). We found that in the keratinocytes of patients with psoriasis, CER[NS], CER[NP], CER[AS], CER[ADS], CER[AP] and CER[EOS] tended to be expressed at higher relative levels, whereas CER[NDS] tended to be expressed with lower levels than in healthy subjects. In the case of fibroblasts, significant differences were observed, mainly in the three ceramide classes (CER[AS], CER[ADS] and CER[EOS]), which were expressed at significantly higher levels in patients with psoriasis. The most significant alteration in the fibroblasts involved elevated levels of CER[EOS] that contained ester-linked fatty acids. Our findings provide insights into the ceramide profile in the dermis and epidermis of patients with psoriasis and contribute for the research in this field, focusing on the role of keratinocyte-fibroblast crosstalk in the development of psoriasis vulgaris.
Effect of the Shape of Styrene–Acrylonitrile Water-Filter Housings on the Destructive Pressure, Crack-Initiation, Propagation Conditions and Fracture Toughness of Styrene–Acrylonitrile
Authors: Zorc Borut , Zorc Matija , Kosec Borut , Nagode Aleš
Published: January 2020

A destructive pressure test of styrene–acrylonitrile (SAN) water-filter housings showed the influence of the shape and specific details of the housings on their critical areas and their destructive pressure. The destructive pressure varies by as much as 37 bar due to different dominant stresses in the individual types of housings. In critical areas of the housings, geometrical stress concentrators generally exist. For this reason, the stress caused by the internal pressure is locally 2.75–3.4 times greater than that expected based on the water pressure, which means that cracks are initiated in these places. However, the bottom of the housings can be in a form such that the maximum stress and the crack originates in its central part without the influence of local stress concentrators. The tensile strength of the SAN is theoretically estimated at 73 N/mm2, which is comparable with the literature data. The fracture toughness of the SAN is typically low, theoretically estimated in the range 1.45–3.55 MPa·m1/2, and strongly depends on the degree of the wall’s stress-increasing rate or the crack-propagation rate. Therefore, at various crack-propagation rates, the critical crack depths are also different, in the range 100–600 μm. Due to this, the critical thickness for brittle fracture in the SAN is also different; it is ten times greater than the critical crack length. The characteristic of a sub-critical crack, i.e., the mirror zone, is its macroscopically smooth surface, which is microscopically very finely roughened. In the case of a sufficiently slowly growing sub-critical crack, the surface of the mirror zone contains characteristic parabolic markings. The over-critical, sufficiently rapidly growing cracks generally grow mainly in the plane-strain state and only the final thin layer of the remaining wall thickness breaks in the plane-stress state. The over-critical, sufficiently slowly growing cracks grow in the plane-stress state with a strong shear plastic tearing.
High-Specificity Digital Architecture for Real-Time Recognition of Loss of Balance Inducing Fall
Authors: De Venuto Daniela , Mezzina Giovanni
Published: January 2020

Falls are a significant cause of loss of independence, disability and reduced quality of life in people with Parkinson’s disease (PD). Intervening quickly and accurately on the postural instability could strongly reduce the consequences of falls. In this context, the paper proposes and validates a novel architecture for the reliable recognition of losses of balance situations. The proposed system addresses some challenges related to the daily life applicability of near-fall recognition systems: the high specificity and system robustness against the Activities of Daily Life (ADL). In this respect, the proposed algorithm has been tested on five different tasks: walking steps, sudden curves, chair transfers via the timed up and go (TUG) test, balance-challenging obstacle avoidance and slip-induced loss of balance. The system analyzes data from wireless acquisition devices that capture electroencephalography (EEG) and electromyography (EMG) signals. The collected data are sent to two main units: the muscular unit and the cortical one. The first realizes a binary ON/OFF pattern from muscular activity (10 EMGs) and triggers the cortical unit. This latter unit evaluates the rate of variation in the EEG power spectrum density (PSD), considering five bands of interest. The neuromuscular features are then sent to a logical network for the final classification, which distinguishes among falls and ADL. In this preliminary study, we tested the proposed model on 9 healthy subjects (aged 26.3 ± 2.4 years), even if the study on PD patients is under investigation. Experimental validation on healthy subjects showed that the system reacts in 370.62 ± 60.85 ms with a sensitivity of 93.33 ± 5.16%. During the ADL tests the system showed a specificity of 98.91 ± 0.44% in steady walking steps recognition, 99.61 ± 0.66% in sudden curves detection, 98.95 ± 1.27% in contractions related to TUG tests and 98.42 ± 0.90% in the obstacle avoidance protocol.
Label-Free Impedimetric Immunosensors Modulated by Protein A/Bovine Serum Albumin Layer for Ultrasensitive Detection of Salbutamol
Authors: Lin Chia-Hung , Lin Ming-Jie , Huang Jie-De , Chuang Yu-Sheng , Kuo Yu-Fen , Chen Jung-Chih , Wu Ching-Chou
Published: January 2020

The sensing properties of immunosensors are determined not only by the amount of immobilized antibodies but also by the number of effective antigen-binding sites of the immobilized antibody. Protein A (PA) exhibits a high degree of affinity with the Fc part of IgG antibody to feasibly produce oriented antibody immobilization. This work proposes a simple method to control the PA surface density on gold nanostructure (AuNS)-deposited screen-printed carbon electrodes (SPCEs) by mixing concentration-varied PA and bovine serum albumin (BSA), and to explore the effect of PA density on the affinity attachment of anti-salbutamol (SAL) antibodies by electrochemical impedance spectroscopy. A concentration of 100 μg/mL PA and 100 μg/mL BSA can obtain a saturated coverage on the 3-mercaptoproponic acid (MPA)/AuNS/SPCEs and exhibit a 50% PA density to adsorb the amount of anti-SAL, more than other concentration-varied PA/BSA-modified electrodes. Compared with the randomly immobilized anti-SAL/MPA/AuNS/SPCEs and the anti-SAL/PA(100 μg/mL):BSA(0 μg/mL)/MPA/AuNS/SPCE, the anti-SAL/PA(100 μg/mL): BSA(100 μg/mL)/MPA/AuNS/SPCE-based immunosensors have better sensing properties for SAL detection, with an extremely low detection limit of 0.2 fg/mL and high reproducibility (<2.5% relative standard deviation). The mixture of PA(100 μg/mL):BSA(100 μg/mL) for the modification of AuNS/SPCEs has great promise for forming an optimal protein layer for the oriented adsorption of IgG antibodies to construct ultrasensitive SAL immunosensors.
IoT Based Architecture for Model Predictive Control of HVAC Systems in Smart Buildings
Authors: Carli Raffaele , Cavone Graziana , Ben Othman Sarah , Dotoli Mariagrazia
Published: January 2020

The efficient management of Heating Ventilation and Air Conditioning (HVAC) systems in smart buildings is one of the main applications of the Internet of Things (IoT) paradigm. In this paper we propose an IoT based architecture for the implementation of Model Predictive Control (MPC) of HVAC systems in real environments. The considered MPC algorithm optimizes on line, in a closed-loop control fashion, both the indoor thermal comfort and the related energy consumption for a single zone environment. Thanks to the proposed IoT based architecture, the sensing, control, and actuating subsystems are all connected to the Internet, and a remote interface with the HVAC control system is guaranteed to end-users. In particular, sensors and actuators communicate with a remote database server and a control unit, which provides the control actions to be actuated in the HVAC system; users can set remotely the control mode and related set-points of the system; while comfort and environmental indices are transferred via the Internet and displayed on the end-users’ interface. The proposed IoT based control architecture is implemented and tested in a campus building at the Polytechnic of Bari (Italy) in a proof of concept perspective. The effectiveness of the proposed control algorithm is assessed in the real environment evaluating both the thermal comfort results and the energy savings with respect to a classical thermostat regulation approach.
Electronic Nose with Digital Gas Sensors Connected via Bluetooth to a Smartphone for Air Quality Measurements
Authors: Arroyo Patricia , Meléndez Félix , Suárez José Ignacio , Herrero José Luis , Rodríguez Sergio , Lozano Jesús
Published: January 2020

This paper introduces a miniaturized personal electronic nose (39 mm × 33 mm), which is managed through an app developed on a smartphone. The electronic nose (e-nose) incorporates four new generation digital gas sensors. These MOx-type sensors incorporate a microcontroller in the same package, being also smaller than the previous generation. This makes it easier to integrate them into the electronics and improves their performance. In this research, the application of the device is focused on the detection of atmospheric pollutants in order to complement the information provided by the reference stations. To validate the system, it has been tested with different concentrations of NOx including some tests specifically developed to study the behavior of the device in different humidity conditions. Finally, a mobile application has been developed to provide classification services. In this regard, a neural network has been developed, trained, and integrated into a smartphone to process the information retrieved from e-nose devices.
Blockchain-based Reputation for Intelligent Transportation Systems
Authors: Hîrţan Liviu-Adrian , Dobre Ciprian , González-Vélez Horacio
Published: January 2020

A disruptive technology often used in finance, Internet of Things (IoT) and healthcare, blockchain can reach consensus within a decentralised network—potentially composed of large amounts of unreliable nodes—and to permanently and irreversibly store data in a tamper-proof manner. In this paper, we present a reputation system for Intelligent Transportation Systems (ITS). It considers the users interested in traffic information as the main actors of the architecture. They securely share their data which are collectively validated by other users. Users can choose to employ either such crowd-sourced validated data or data generated by the system to travel between two locations. The data saved is reliable, based on the providers’ reputation and cannot be modified. We present results with a simulation for three cities: San Francisco, Rome and Beijing. We have demonstrated the impact of malicious attacks as the average speed decreased if erroneous information was stored in the blockchain as an implemented routing algorithm guides the honest cars on other free routes, and thus crowds other intersections.
The Role of Consumers in the Achievement of Corporate Sustainability through the Reduction of Unfair Commercial Practices
Authors: Mihajlović Borko
Published: January 2020

The subject of this article is a determination of the potential of the prohibition of unfair commercial practice, as an institution of consumer law, established to strengthen consumers’ protection, to contribute to the achievement of corporate sustainability. The main objective of the paper is the identification of situations in which certain companies’ actions may at the same time be considered as unsustainable and socially irresponsible behavior, as well as unfair commercial practice. There are three such characteristic situations that were the subject of thorough analysis in the paper: (1) company’s constantly invoking to the concepts of corporate sustainability and corporate social responsibility (CSR) in its marketing activities, which is essentially incorrect because the company in practice breaches some of the basic postulates of these concepts, (2) breach of company’s code of conduct which contains the principles of corporate sustainability and CSR, and which the company has undertaken to be bound, (3) conducting many concrete acts which represent unfair commercial practice, and simultaneously have negative impact on the corporate sustainability and CSR. From the analysis of these three situations arises the conclusion that there is a possibility for indirect legal sanctioning of the unsustainable and socially irresponsible behavior of companies through the application of the consumer law rules on the prohibition of unfair commercial practice.
Aplicação da metodologia SMED para redução no tempo de setup na produção de embalagens cartonadas
Authors: José Ceron Branco Fábio , Pereira Silva Eduardo
Published: January 2020

Este trabalho apresenta um estudo de caso que utiliza de uma das ferramentas do sistema de gestão TPM (Total Productive Maintenance) ou "Manutenção Produtiva Total", conhecida como SMED (Single Minute Exchange of Die) "Troca de ferramental em apenas um digito de minuto", criada por Shigeo Shingo. Tem como objetivo propor um modelo de aplicação desta metodologia para auxiliar fábricas de qualquer ramo que tenham em seus processos de produção lotes diferenciados a obterem alta flexibilidade com a redução em seus tempos de troca de ferramental, denominado SETUP, estando assim aptas a corresponderem às demandas de seus clientes por lotes cada vez menores. Conclui-se esta pesquisa enfatizando-se que com a implantação dos passos desta metodologia, foi possível alcançar resultados financeiros expressivos, tangíveis no momento em que possibilita minimizar perdas que impactam diretamente nos KPI (Key Process Indicators) "indicadores chaves de processo", como abordado neste estudo, como o Overall equipment effectiveness (OEE) "indicador global de eficiência" de uma linha de produção flexográfica de uma empresa de embalagens cartonadas, tal como definiu-se um padrão único de operação para que se mantivesse este indicador em seu nível tecnológico mantido e de fácil execução.
The epistemological crisis of Marxian economic theory
Authors: Petersen Matias
Published: January 2020

In Ethics in the Conflicts of Modernity, MacIntyre argues that neo-Aristotelians have much to learn from Marx’s economic theory, not only for understanding the nature of capitalism, but also for thinking about alternative social and political institutions. This article outlines the arguments given by MacIntyre for embracing Marxian economic theory and argues that if Marxian economics is a tradition of enquiry, in the MacIntyrean sense of the term, we should take seriously the debates within this tradition in order to conclude whether it has been able to withstand internal and external criticism. I argue that Marxian economic theory, as a tradition of enquiry, has been defeated by its opponents and that a synthesis between Aristotelian moral philosophy and Marxian economics is an obstacle to the development of MacIntyre’s political philosophy.