bluuebunny/crossref_metadata_2025 huggingface dataset:
The last-mile vehicle routing problem with delivery options
Authors: Tilk Christian , Olkis Katharina , Irnich Stefan
Published: June 2021

The ongoing rise in e-commerce comes along with an increasing number of first-time delivery failures due to the absence of the customer at the delivery location. Failed deliveries result in rework which in turn has a large impact on the carriers’ delivery cost. In the classical vehicle routing problem (VRP) with time windows, each customer request has only one location and one time window describing where and when shipments need to be delivered. In contrast, we introduce and analyze the vehicle routing problem with delivery options (VRPDO), in which some requests can be shipped to alternative locations with possibly different time windows. Furthermore, customers may prefer some delivery options. The carrier must then select, for each request, one delivery option such that the carriers’ overall cost is minimized and a given service level regarding customer preferences is achieved. Moreover, when delivery options share a common location, e.g., a locker, capacities must be respected when assigning shipments. To solve the VRPDO exactly, we present a new branch-price-and-cut algorithm. The associated pricing subproblem is a shortest-path problem with resource constraints that we solve with a bidirectional labeling algorithm on an auxiliary network. We focus on the comparison of two alternative modeling approaches for the auxiliary network and present optimal solutions for instances with up to 100 delivery options. Moreover, we provide 17 new optimal solutions for the benchmark set for the VRP with roaming delivery locations.
Accuracy of high-sensitive troponin depending on renal function for clinical outcome prediction in patients with acute heart failure
Authors: Ledwoch Jakob , Krauth Anna , Kraxenberger Jana , Schneider Alisa , Leidgschwendner Katharina , Schneider Vera , Müller Alexander , Laugwitz Karl-Ludwig , Kupatt Christian , Martens Eimo
Published: June 2021

High-sensitive troponin T (hs-TnT) is increasingly used for clinical outcome prediction in patients with acute heart failure (AHF). However, there is an ongoing debate regarding the potential impact of renal function on the prognostic accuracy of hs-TnT in this setting. The aim of the present study was to assess the prognostic value of hs-TnT within 6 h of admission for the prediction of 30-day mortality depending on renal function in patients with AHF. Patients admitted to our institution due to AHF were retrospectively included. Clinical information was gathered from electronic and paper-based patient charts. Patients with myocardial infarction were excluded. A total of 971 patients were enrolled in the present study. A negative correlation between estimated glomerular filtration rate (eGFR) and hsTnT was identified (Pearson r = − 0.16; p < 0.001) and eGFR was the only variable to be independently associated with hsTnT. The area under the curve (AUC) of hs-TnT for the prediction of 30-mortality was significantly higher in patients with an eGFR ≥ 45 ml/min (AUC 0.74) compared to those with an eGFR < 45 ml/min (AUC 0.63; p = 0.049). Sensitivity and specificity of the Youden Index derived optimal cut-off for hs-TnT was higher in patients with an eGFR ≥ 45 ml/min (40 ng/l: sensitivity 73%, specificity 71%) compared to patients with an eGFR < 45 ml/min (55 ng/l: sensitivity 63%, specificity 62%). Prognostic accuracy of hs-TnT in patients hospitalized for AHF regarding 30-day mortality is significantly lower in patients with reduced renal function.
Tree species, crown cover, and age as determinants of the vertical distribution of airborne LiDAR returns
Authors: Racine Etienne B. , Coops Nicholas C. , Bégin Jean , Myllymäki Mari
Published: June 2021

Key message

We assessed even-aged stand vertical distributions of LiDAR returns and found that tree species, age, and crown cover each have a distinct pattern that together explains up to 47% of the variation.

Light detection and ranging (LiDAR) provides information on the vertical structure of forest stands enabling detailed and extensive ecosystem study. The vertical structure is often summarized by scalar features and data-reduction techniques that limit the interpretation of results. Instead, we quantified the influence of three variables, species, crown cover, and age, on the vertical distribution of airborne LiDAR returns from forest stands. We studied 5428 regular, even-aged stands in Quebec (Canada) with five dominant species: balsam fir [Abies balsamea(L.) Mill.], paper birch (Betula papyriferaMarsh), black spruce [Picea mariana(Mill.) BSP], white spruce (Picea glaucaMoench) and aspen (Populus tremuloidesMichx.). We modeled the vertical distribution against the three variables using a functional general linear model and a novel nonparametric graphical test of significance. Results indicate that LiDAR returns from aspen stands had the most uniform vertical distribution. Balsam fir and white birch distributions were similar and centered at around 50% of the stand height, and black spruce and white spruce distributions were skewed to below 30% of stand height ($$p$$p<0.001). Increased crown cover concentrated the distributions around 50% of stand height. Increasing age gradually shifted the distributions higher in the stand for stands younger than 70-years, before plateauing and slowly declining at 90–120 years. Results suggest that the vertical distributions of LiDAR returns depend on the three variables studied.
Excess short-term mortality in noncritical patients with atrial fibrillation presenting to the emergency department
Authors: Niederdöckl Jan , Schwameis Michael , Herkner Harald , Domanovits Hans
Published: June 2021

Background

Mortality data of non-critically ill patients presenting with symptomatic atrial fibrillation to an emergency department are scarce. We aimed to analyze the short-term mortality of these patients compared with that of the general Austrian population.

Design/methods

This study analyzed a consecutive series of non-critically ill adults presenting to the emergency department at the Medical University of Vienna between 2012 and 2016 with complaints related to atrial fibrillation. The study outcome was mortality during the observation period. Age-specific and sex-specific mortality rates per 100 person-years were calculated and compared with the mortality rates of the Austrian population during the same period.

Results

In total, 1754 patients with atrial fibrillation (43.1% female) were included in the study. During a median follow-up of 25 months, 248 of these patients died. Observed mortality rates were 7.8 per 100 person-years for females (95% confidence interval, CI 6.6–9.5) and 5.9 per 100 person-years for males (95% CI 5.0–7.1). Age-adjusted and sex-adjusted mortality rates were 2.8 (95% CI 2.3–3.3) and 2.7 (95% CI 2.2–3.2) per 100 person-years, respectively. Mortality rates for the Austrian population were 1.1 per 100 person-years for both females and males. Corresponding standardized mortality ratios were 2.5 for females (95% CI 2.1–3.0) and 2.4 for males (95% CI 2.0–2.9).

Conclusion

The short-term mortality of patients with symptomatic atrial fibrillation presenting to the emergency department was substantially higher compared with the general Austrian population.
Shallow groundwater chemical evolution, isotopic hyperfiltration, and salt pan formation in a hypersaline endorheic basin: Pilot Valley, Great Basin, USA
Authors: Mayo A. L. , Tingey D. G.
Published: June 2021

Endorheic basin brines are of economic significance as sources of boron, iodine, magnesium, potassium, sodium sulfate, sodium carbonate, and tungsten, and they are a major source of the critical metal lithium. Although evaporation is the primary hypersalinization driver for evaporative water bodies, recent investigations have proposed more novel mechanisms for some subsurface brine. This investigation explores shallow groundwater hypersalinization. The chemical evolution and isotopic fractionation of shallow hypersaline groundwater in the clay-rich arid endorheic basin sediments of Pilot Valley, Great Basin (USA), were investigated. Groundwater evolves from fresh in the mountain bedrock and alluvial fans, to brackish and saline at the alluvial fan–playa interface, and to hypersaline in the upper 12 m of basin sediments. Alluvial fan systems are isolated from each other and have varying groundwater3H and14C travel times. Nonevaporative in-situ isotopic fractionation of up to −8‰ in δ18O is attributed to clay sequence hyperfiltration. Groundwater flow-path sulfate and chloride mineral dissolution is the primary driving mechanism for both interface and basin groundwater evolution. Evaporation only impacts the groundwater quality in a small portion of the basin where the groundwater is within ~1 m of the ground surface. Here capillary action carries dissolved soluble salts to the land surface. Episodic flooding redissolves and carries the precipitated salt to the annually flooded salt pan where it accumulates as a salt crust during the dry season. The Pilot Valley model may help explain the buildup accumulative layers of soluble salt that when remobilized becomes subsurface brine.
Assessing TBM performance in heterogeneous rock masses
Authors: Sissins Sarah , Paraskevopoulou Chrysothemis
Published: June 2021

A major challenge that TBM performance is requested to deal with for a successful and effective progress is tunnelling through lithologically and geomechanically heterogeneous rock masses. Such heterogeneous environments are common and recent tunnel examples in the UK include the Hinckley Point C offshore cooling tunnels being driven through interbedded carbonaceous mudstone/shales and argillaceous limestone and the Anglo American’s Woodsmith Mine Mineral Transport System tunnel in Redcar Mudstone with beds of ironstone. This inherent geological heterogeneity leads to difficult tunnelling conditions that initially stem from predicting a sound and representative ground model that can be used to preliminary assess the TBM performance. In this work, an exhaustive review of existing TBM Penetration Rate (PR) methods identified that no models address the issue of parameter selection for heterogeneous rock masses comprising layers with different rock strengths. Consequently, new approaches are required for estimating rock mass behaviour and machine performance in such environments. In the presented work the Blue Lias Formation (BLI), which is characterised by its layered rock mass, comprising very strong limestone, interbedded with weak mudstone and shales, is investigated. BLI formation is considered herein being a representative example of lithological heterogeneity. Based on the fieldwork carried out in three localities in the Bristol Channel Basin (S. Wales and Somerset), geological models are produced based on which a geotechnical model is developed, and four ground types are determined. Implications of the current findings for TBM performance are assessed, including faulting, groundwater inflow and excavation stability with a particular focus on both PR and advance rate. A modified approach using the existing empirical models is proposed, developed and presented in this paper that can be used as a guide to determine TBM performance in heterogeneous rock masses reducing the risk of cost and time overruns.
Freezing: how do water mites (Acari: Hydrachnidia) survive exposure to sub-zero temperatures?
Authors: Zawal Andrzej , Czernicki Tomasz , Michoński Grzegorz , Bańkowska Aleksandra , Stryjecki Robert , Pešić Vladimir , Achrem Magdalena , Skorupski Jakub , Pakulnicka Joanna , Szlauer-Łukaszewska Agnieszka
Published: June 2021

Until now, very little is known about the ability of adult and deutonymph water mites (Acari, Hydrachnidia) to survive in sub-zero temperatures. Information concerns mainly water mites from vernal astatic waters, and the knowledge has never been experimentally verified. To determine the sensitivity of water mites to freezing, experiments were conducted on (1) the impact of acclimatization, (2) temperature, and (3) duration of freezing on survival, (4) the survival rate of water mites from various types of water bodies, and (5) the survival rate of water mites from different climatic zones. The experiments were carried out in a phytotron chamber, and water mites were placed in containers (10 × 10 × 5 cm) filled with 4/5 of water for 10 specimens each. Water mites were identified to the species level after finishing the experiments. The temperature was lowered 1 °C every hour until the target temperature was reached. After a certain period of freezing (depending on the treatment) the temperature was raised by 1 °C every hour until it reached 4 °C. The time of the experiment was measured from the moment the desired temperature was reached (below 0 °C) until the ice thawed and the temperature of 4 °C was reached again. The highest survival rates had Limnochares aquatica, Piona nodata, Sperchon clupeifer and Lebertia porosa, followed by L. insignis, Hygrobates longipalpis, H. setosus, Limnesia undulatoides, Piona pusilla, Arrenurus globator, Hydrodroma despiciens, Piona longipalpis, Sperchonopsis verrucosa, Unionicola crassipes and Mideopsis crassipes; no specimens of Torrenticola amplexa survived. The following conclusions were drawn: (1) water mites can survive freezing to −2 °C, lower temperatures are lethal for them; (2) they survived better the short period of freezing (24–48 h) than the long period (168 h); (3) resistance to freezing seems to be an evolutionary trait of individual species, only partly related to the living environment; and (4) freezing survival rates are linked to the region of Europe and are much lower in Southern than in Central Europe.
The Effects of Choice on the Reading Comprehension and Enjoyment of Children with Severe Inattention and no Attentional Difficulties
Authors: Kakoulidou Myrofora , Le Cornu Knight Frances , Filippi Roberto , Hurry Jane
Published: June 2021

It has been proposed that enhancing motivation supports the learning of children with Attention Deficit Hyperactivity Disorder (ADHD). Less is known if inattentive children with no ADHD diagnosis may similarly benefit, when being motivated to engage in an academic task. Using a repeated-measures design, this study investigated the effects of text choice as an intrinsic motivator on the reading comprehension and enjoyment of Year 4 children attending mainstream primary schools (N = 92; aged 8–9 years; 48 boys); comparing those with no attentional difficulties and severe inattention. We hypothesized that 1) choice would increase reading comprehension and enjoyment 2) choice would increase the reading comprehension and enjoyment of children both with severe inattention and no attentional difficulties 3) choice effects would be significantly greater for children with severe inattention than those with no attentional difficulties. Children participated in a reading intervention that included a Choice (experimental) and a No Choice (control) condition. Child inattention was measured via a Virtual Reality Continuous Performance Task (Omission errors, Reaction Time Variability) and Teacher Ratings. Choice significantly increased reading comprehension, but not enjoyment compared with no choice. Choice improved the reading comprehension of children with both severe inattention and no attentional difficulties. Choice did not benefit the reading of severely inattentive children more than that of children with no attentional difficulties. These findings underline the educational benefits of choice for young readers both with severe inattention and no attentional difficulties, which are further discussed drawing on existing theory and research.
A qualitative study of the views of healthcare professionals on providing vaccines information to patients
Authors: Loftus Ruth , Sahm Laura J. , Fleming Aoife
Published: June 2021

Background Healthcare professionals (HCPs) such as pharmacists, general practitioners and practice nurses are a trusted source of vaccines information for patients in primary care. Global regulators have highlighted the key role of HCPs in fostering confidence in COVID-19 vaccines. Objective This study aims to gain insight into the views and experiences of HCPs on providing vaccines information to patients. Setting Primary care general practice surgeries and community pharmacies in Ireland. Methods Qualitative, semi-structured interviews were conducted with 14 HCPs (five General practitioners [GPs], four practice nurses and five community pharmacists) identified through purposive and convenience sampling. The interviews were analysed by inductive thematic analysis. Main outcome measure Participants’ views and experiences of providing vaccines information to patients. Results Five key themes were identified: roles and responsibilities, perception of risk, perception of the public, building a relationship, and emotion. HCPs were motivated by duty and care for their patients. They respected patient autonomy and were driven by their concern for public health. HCPs were influenced by their perception of risk and their perceptions of the public. HCPs practiced patient-centred care by providing tailored vaccines information. They favoured an approach of providing patients with information and support to make their own decision. The topic was emotive; HCPs empathised with patients but were also frustrated by their perceived inability to change some patients’ views. Conclusion The provision of vaccines information by HCPs to patients is multifactorial with participants mindful of patient autonomy and the HCP role to support vaccinations as a public health priority. Participants suggested that education and support on vaccines communication would enable them to support the vaccines uptake in their practice.
Curriculum and the Covid-19 crisis
Authors: Pinar William F.
Published: June 2021

The article argues that the Covid-19 crisis is a curriculum crisis, because it is a humanitarian crisis. Survival—physical, psychological, educational—is at stake. As educators have mobilized to meet the emergency, this introductory article to Prospects special issue on Curriculum Responsiveness to Crisis glimpses elements of that effort, both theoretical and practical. It concludes that the student—the individual person—should remain central to any conception of curriculum, to any organization of pedagogical communication, indeed to the very project of education itself.
BMI and dissatisfaction with life: contextual factors and socioemotional costs of obesity
Authors: Jarosz Ewa , Gugushvili Alexi
Published: June 2021

Purpose

In this study, we investigate whether individuals’ BMI categories are associated with being dissatisfied with one’s life, how this association is affected by the social comparison that individuals make, and what the role of the overall BMI levels in this process is.

Methods

We use data for 21,577 men and 27,415 women, collected in 2016 by the European Bank for Reconstruction and Development, from 34 countries in Europe, the Middle East, and Central Asia. To understand the moderating effect of contextual environment, we use multilevel mixed effect logistic regression models and data for national, regional, and cohort-specific BMI levels.

Result

We find that the association of BMI and dissatisfaction with life differs by gender, with overweight men being less likely to be dissatisfied with life than men with normal weight and obese women being more likely to be dissatisfied with life compared to women with normal weight. For contextual effects, we find that obese women in regions with low BMI levels are more likely to be dissatisfied with life. The effect of obesity on female life dissatisfaction is not observed in regions with high BMI levels. As for men, regional BMI levels affect the levels of life dissatisfaction but only for underweight men.

Conclusions

Our study adds additional nuance to the quality-of-life research by showing that the association between BMI and decreased life satisfaction is, at least partially, moderated by the contextual environment, and that the character of these effects differs by gender.
Metal–Organic Framework-Based Solid Acid Materials for Biomass Upgrade
Authors: Qin Yutian , Guo Jun , Zhao Meiting
Published: June 2021

Biomass is a green and producible source of energy and chemicals. Hence, developing high-efficiency catalysts for biomass utilization and transformation is urgently demanded. Metal–organic framework (MOF)-based solid acid materials have been considered as promising catalysts in biomass transformation. In this review, we first introduce the genre of Lewis acid and Brønsted acid sites commonly generated in MOFs or MOF-based composites. Then, the methods for the generation and adjustment of corresponding acid sites are overviewed. Next, the catalytic applications of MOF-based solid acid materials in various biomass transformation reactions are summarized and discussed. Furthermore, based on our personal insights, the challenges and outlook on the future development of MOF-based solid acid catalysts are provided. We hope that this review will provide an instructive roadmap for future research on MOFs and MOF-based composites for biomass transformation.
Prevalence of GII.4 Sydney Norovirus Strains and Associated Factors of Acute Gastroenteritis in Children: 2019/2020 Season in Guangzhou, China
Authors: Duan Lei , Yang Xiaohan , Xie Jia , Zhan Wenli , Zhang Changbin , Liu Hong , Wei Mengru , Tang Yuan , Zhao Hongyu , Luo Mingyong
Published: June 2021

Norovirus, the leading cause of non-bacterial acute gastroenteritis (AGE) worldwide, is constantly mutating. Continuous monitoring of the evolution of epidemic genotypes and emergence of novel genotypes is, therefore, necessary. This study determined the prevalence and clinical characteristics of norovirus strains in AGE in Guangzhou, China in 2019/2020 season. This study included children aged 2–60 months diagnosed with AGE in Guangzhou Women and Children Hospital, from August 2019 to January 2020. Norovirus was detected by real-time polymerase chain reaction and clinical data were obtained. Genotyping and phylogenetic analyses were performed with partial gene sequence fragments located within the open reading frames 1 and 2. During the study period, 168 children (61.3% males) were confirmed as norovirus infectious AGE. The main symptoms were diarrhoea and vomiting and 38 patients (22.6%) had seizures. Norovirus was mainly prevalent in October and November, and GII.4 Sydney[P31] was the major genotype circulating in Guangzhou. The phylogenetic tree showed that the Guangzhou strains had high homology with the strains circulating in 2017–2019 worldwide. GII.4 Sydney was the main prevalent norovirus genotype in Guangzhou from August 2019 to January 2020, which had more severe diarrhoea than those of other genotypes. These findings provide a valuable reference for the prevention, control, and treatment of norovirus in the future.
The retreat of the delta: a geomorphological history of the Po river basin during the twentieth century
Authors: Parrinello Giacomo , Bizzi Simone , Surian Nicola
Published: June 2021

The morphology of rivers and deltas, like many features of the Earth’s physical geography, is today subject to dramatic and rapid changes due to human actions. Deprived of sediment from their basins and besieged by rising sea levels, many deltas are at risk of complete disappearance. Despite a rich historical scholarship on rivers, we know little about the history of these important geomorphological processes. This paper sheds light on the geomorphological history of rivers by investigating the case of the Po River basin and its delta during the twentieth century. By combining the insights of fluvial geomorphology and a historical methodology, the paper analyses three main drivers of geomorphic alterations in the catchment that had an impact on the delta: hydroelectricity, sand and gravel mining, and methane extraction. In each case, it focuses on how experts, policy-makers, and overseers understood and regulated (or not) these geomorphic alterations. During much of the twentieth century, engineers and hydrologists monitored geomorphic processes with increasing detail, while state and business actors practiced multiple forms of sediment management. For most of the twentieth century, however, experts did not acknowledge the scale and nature of human-induced geomorphic alteration. Sediment management, moreover, did not take into account sediment scarcity until late in the century, and remained exclusively motivated by local concerns. Through this particular case, this paper offers insights on the historical limits to environmental expertise and policy when facing long term and large-scale geomorphic processes, and encourages a more sustained incorporation of fluvial geomorphology into the history of water systems.
Can group rights justify the denial of education to children? The Amish in the United States as a case study
Authors: Cohen-Almagor Raphael
Published: June 2021

Multiculturalism gives preference to group rights over individual rights. This may challenge democratic values. This paper focuses on the Amish denial of education from their adolescents. CriticizingWisconsin v. Yoder(Wisconsin v. Yoder406 U.S. 205 (1972)), the paper analyses the power of the Amish community over its members. The main questions are: Is it reasonable to deny the Amish adolescents’ standard American education? What are the limits of state interference in norms of illiberal communities who invoke separatism as a mechanism of cultural and religious preservation?
Effect of Mediterranean diet on liver enzymes: a systematic review and meta-analysis of randomised controlled trials
Authors: Sangouni Abbas Ali , Hassani Zadeh Shirin , Mozaffari-Khosravi Hassan , Hosseinzadeh Mahdieh
Published: June 2021

Elevated levels of liver enzymes are the main markers of liver dysfunction. Liver enzymes are the important indicators of non-alcoholic fatty liver disease (NAFLD) in the general population. Previous randomised clinical trials (RCT) investigated the effects of Mediterranean diet (MedDiet) as a plant-based diet on features of NAFLD like liver enzymes, but their results are contradictory. This study aimed to systematically review and meta-analyse RCT investigating the effect of MedDiet on liver enzymes. PubMed, Web of Science, Scopus and Google Scholar were searched until December 2020. A total of ten RCT (n705 participants) evaluating the effect of MedDiet on liver enzymes including aspartate aminotransferase (AST), alanine transaminase (ALT) andγ-glutamyltransferase (GGT) were included. A random effects model was used to estimate the pooled effect size. To evaluate the heterogeneity among the included studies, the Cochran’sQ-test and I-squared test were used. The MedDiet significantly reduced AST (weighted mean difference (WMD) = −0·38 IU/l; 95 % CI − 0·73, −0·03 IU/l;P= 0·03) and GGT (WMD = −0·16 IU/l; 95 % CI − 0·32, −0·006 IU/l;P= 0·04) but had no significant effect on ALT (WMD = −0·55 IU/l; 95 % CI − 1·25, 0·13 IU/l;P= 0·11). However, sensitivity analysis revealed that the overall effects of MedDiet on AST, GGT and ALT were significantly influenced by removing some studies. There was no publication bias based on Begg’s and Egger’s tests. Generally, MedDiet can improve liver enzymes. To better conclusion, further RCT investigating the effect of MedDiet on liver enzymes, especially in patients with NAFLD, are still required.
Loss of the central rachis and synaptonemal complexes during meiotic prophase in female Ascaris lumbricoides var. suum after exposure to albendazole
Authors: Goldstein P.
Published: June 2021

Albendazole, a benzimidazole anthelmintic, interferes with the formation of microtubules and inhibits meiosis in the nematode Ascaris lumbricoides var. suum. Pigs treated with albendazole had worms in their uteri that had a severely deteriorated central rachis, complete loss of synaptonemal complexes and irregular oocytes at meiotic prophase I. The nuclear matrix and envelope were poorly formed and there was formation of accessory nuclei. This study represents the first examination of the changes in meiotic nuclear architecture and meiotic chromosomes after exposure to albendazole. These results provide the basis for the loss of fecundity in A. suum after exposure to albendazole resulting in control in the population of the parasitic nematode.
Species distribution modelling of the Southern Ocean benthos: a review on methods, cautions and solutions
Authors: Guillaumot Charlène , Danis Bruno , Saucède Thomas
Published: June 2021

Species distribution modelling studies the relationship between species occurrence records and their environmental setting, providing a valuable approach to predicting species distribution in the Southern Ocean (SO), a challenging region to investigate due to its remoteness and extreme weather and sea conditions. The specificity of SO studies, including restricted field access and sampling, the paucity of observations and difficulties in conducting biological experiments, limit the performance of species distribution models. In this review, we discuss some issues that may influence model performance when preparing datasets and calibrating models, namely the selection and quality of environmental descriptors, the spatial and temporal biases that may affect the quality of occurrence data, the choice of modelling algorithms and the spatial scale and limits of the projection area. We stress the importance of evaluating and communicating model uncertainties, and the most common evaluation metrics are reviewed and discussed accordingly. Based on a selection of case studies on SO benthic invertebrates, we highlight important cautions to take and pitfalls to avoid when modelling the distribution of SO species, and we provide some guidelines along with potential methods and original solutions that can be used for improving model performance.
Household and child food insecurity and CVD risk factors in lower-income adolescents aged 12–17 years from the National Health and Nutrition Examination Survey (NHANES) 2007–2016
Authors: Fulay Aarohee P , Vercammen Kelsey A , Moran Alyssa J , Rimm Eric B , Leung Cindy W
Published: June 2021

Objective:

Household food insecurity is associated with CVD risk factors in low-income adults, but research on these associations among adolescents is inconsistent. This study investigates whether household and child food insecurity is associated with CVD risk factors in lower-income adolescents.

Design:

Cross-sectional. Multivariable linear regression assessed the association between household and child food security and CVD risk factors. Household and child food security was measured using the US Food Security Survey Module. The analyses were adjusted for adolescent’s age, sex, race/ethnicity, smoking status, physical activity and sedentary time, as well as household income and the head-of-household’s education and marital status.

Setting:

The USA.

Participants:

The sample was comprised of 2876 adolescents, aged 12–17 years, with household incomes at or below 300 % federal poverty line from the National Health and Nutrition Examination Survey cycles 2007–2016.

Results:

The weighted prevalence of household food insecurity in the analytic sample was 33·4 %, and the weighted prevalence of child food insecurity was 17·4 %. After multivariable adjustment, there were no significant associations between household and child food insecurity and BMI-for-age Z-score, systolic and diastolic blood pressure, HDL-cholesterol, total cholesterol, fasting TAG, fasting LDL-cholesterol and fasting plasma glucose.

Conclusions:

Despite observed associations in adults, household food insecurity was not associated with CVD risk factors in a national sample of lower-income adolescents. Child food insecurity was also not associated with CVD risk factors. More research should be conducted to confirm these associations.
The Social Foundations of Positivism: The Case of Late-Nineteenth-Century Italy
Authors: Riley Dylan , Emigh Rebecca Jean , Ahmed Patricia
Published: June 2021

What social conditions produce positivism? One position, common to both positivists and some of their major critics, suggests that positivism is an “ideology” or “worldview” of industrial capitalism. Positivism therefore resonates with the basic experience of capitalism for all social groups. Intellectuals draw on this experience in formulating positivist social science. A second position suggests that positivism is a strategy of distinction by which intellectuals attempt to accumulate symbolic capital against their rivals. This position suggests that positivism is a resource for establishing a social science that imitates the methodology of natural science. Our article argues for a third view focused on the internal structure of the intelligentsia as a social group. Positivism could emerge in both industrial capitalist and preindustrial contexts; however, the types of positivism differ in these two cases because the structure of the intelligentsia differs. In preindustrial contexts, such as nineteenth-century Italy, which is the focus of our analysis, positivists claim an ontological continuity between natural and social sciences. In industrial contexts, on the basis of which most theories of positivism rest, positivists claim a methodological similarity between natural and social sciences. We conclude our analysis by reflecting on the implications of our study for work on positivism and social ontology in the social sciences.
METTL3 promotes tumour development by decreasing APC expression mediated by APC mRNA N6-methyladenosine-dependent YTHDF binding
Authors: Wang Wei , Shao Fei , Yang Xueying , Wang Juhong , Zhu Rongxuan , Yang Yannan , Zhao Gaoxiang , Guo Dong , Sun Yingli , Wang Jie , Xue Qi , Gao Shugeng , Gao Yibo , He Jie , Lu Zhimin
Published: June 2021

The adenomatous polyposis coli (APC) is a frequently mutated tumour suppressor gene in cancers. However, whether APC is regulated at the epitranscriptomic level remains elusive. In this study, we analysed TCGA data and separated 200 paired oesophageal squamous cell carcinoma (ESCC) specimens and their adjacent normal tissues and demonstrated that methyltransferase-like 3 (METTL3) is highly expressed in tumour tissues. m6A-RNA immunoprecipitation sequencing revealed that METTL3 upregulates the m6A modification of APC, which recruits YTHDF for APC mRNA degradation. Reduced APC expression increases the expression of β-catenin and β-catenin-mediated cyclin D1, c-Myc, and PKM2 expression, thereby leading to enhanced aerobic glycolysis, ESCC cell proliferation, and tumour formation in mice. In addition, downregulated APC expression correlates with upregulated METTL3 expression in human ESCC specimens and poor prognosis in ESCC patients. Our findings reveal a mechanism by which the Wnt/β-catenin pathway is upregulated in ESCC via METTL3/YTHDF-coupled epitranscriptomal downregulation of APC.
A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker
Authors: Bozhilov Yavor K. , Downes Damien J. , Telenius Jelena , Marieke Oudelaar A. , Olivier Emmanuel N. , Mountford Joanne C. , Hughes Jim R. , Gibbons Richard J. , Higgs Douglas R.
Published: June 2021

Many single nucleotide variants (SNVs) associated with human traits and genetic diseases are thought to alter the activity of existing regulatory elements. Some SNVs may also create entirely new regulatory elements which change gene expression, but the mechanism by which they do so is largely unknown. Here we show that a single base change in an otherwise unremarkable region of the human α-globin cluster creates an entirely new promoter and an associated unidirectional transcript. This SNV downregulates α-globin expression causing α-thalassaemia. Of note, the new promoter lying between the α-globin genes and their associated super-enhancer disrupts their interaction in an orientation-dependent manner. Together these observations show how both the order and orientation of the fundamental elements of the genome determine patterns of gene expression and support the concept that active genes may act to disrupt enhancer-promoter interactions in mammals as in Drosophila. Finally, these findings should prompt others to fully evaluate SNVs lying outside of known regulatory elements as causing changes in gene expression by creating new regulatory elements.
SARS-CoV-2-mediated dysregulation of metabolism and autophagy uncovers host-targeting antivirals
Authors: Gassen Nils C. , Papies Jan , Bajaj Thomas , Emanuel Jackson , Dethloff Frederik , Chua Robert Lorenz , Trimpert Jakob , Heinemann Nicolas , Niemeyer Christine , Weege Friderike , Hönzke Katja , Aschman Tom , Heinz Daniel E. , Weckmann Katja , Ebert Tim , Zellner Andreas , Lennarz Martina , Wyler Emanuel , Schroeder Simon , Richter Anja , Niemeyer Daniela , Hoffmann Karen , Meyer Thomas F. , Heppner Frank L. , Corman Victor M. , Landthaler Markus , Hocke Andreas C. , Morkel Markus , Osterrieder Nikolaus , Conrad Christian , Eils Roland , Radbruch Helena , Giavalisco Patrick , Drosten Christian , Müller Marcel A.
Published: June 2021

Viruses manipulate cellular metabolism and macromolecule recycling processes like autophagy. Dysregulated metabolism might lead to excessive inflammatory and autoimmune responses as observed in severe and long COVID-19 patients. Here we show that SARS-CoV-2 modulates cellular metabolism and reduces autophagy. Accordingly, compound-driven induction of autophagy limits SARS-CoV-2 propagation. In detail, SARS-CoV-2-infected cells show accumulation of key metabolites, activation of autophagy inhibitors (AKT1, SKP2) and reduction of proteins responsible for autophagy initiation (AMPK, TSC2, ULK1), membrane nucleation, and phagophore formation (BECN1, VPS34, ATG14), as well as autophagosome-lysosome fusion (BECN1, ATG14 oligomers). Consequently, phagophore-incorporated autophagy markers LC3B-II and P62 accumulate, which we confirm in a hamster model and lung samples of COVID-19 patients. Single-nucleus and single-cell sequencing of patient-derived lung and mucosal samples show differential transcriptional regulation of autophagy and immune genes depending on cell type, disease duration, and SARS-CoV-2 replication levels. Targeting of autophagic pathways by exogenous administration of the polyamines spermidine and spermine, the selective AKT1 inhibitor MK-2206, and the BECN1-stabilizing anthelmintic drug niclosamide inhibit SARS-CoV-2 propagation in vitro with IC50 values of 136.7, 7.67, 0.11, and 0.13 μM, respectively. Autophagy-inducing compounds reduce SARS-CoV-2 propagation in primary human lung cells and intestinal organoids emphasizing their potential as treatment options against COVID-19.
Systematic detection of functional proteoform groups from bottom-up proteomic datasets
Authors: Bludau Isabell , Frank Max , Dörig Christian , Cai Yujia , Heusel Moritz , Rosenberger George , Picotti Paola , Collins Ben C. , Röst Hannes , Aebersold Ruedi
Published: June 2021

To a large extent functional diversity in cells is achieved by the expansion of molecular complexity beyond that of the coding genome. Various processes create multiple distinct but related proteins per coding gene – so-called proteoforms – that expand the functional capacity of a cell. Evaluating proteoforms from classical bottom-up proteomics datasets, where peptides instead of intact proteoforms are measured, has remained difficult. Here we present COPF, a tool for COrrelation-based functional ProteoForm assessment in bottom-up proteomics data. It leverages the concept of peptide correlation analysis to systematically assign peptides to co-varying proteoform groups. We show applications of COPF to protein complex co-fractionation data as well as to more typical protein abundance vs. sample data matrices, demonstrating the systematic detection of assembly- and tissue-specific proteoform groups, respectively, in either dataset. We envision that the presented approach lays the foundation for a systematic assessment of proteoforms and their functional implications directly from bottom-up proteomic datasets.
Active coacervate droplets are protocells that grow and resist Ostwald ripening
Authors: Nakashima Karina K. , van Haren Merlijn H. I. , André Alain A. M. , Robu Irina , Spruijt Evan
Published: June 2021

Active coacervate droplets are liquid condensates coupled to a chemical reaction that turns over their components, keeping the droplets out of equilibrium. This turnover can be used to drive active processes such as growth, and provide an insight into the chemical requirements underlying (proto)cellular behaviour. Moreover, controlled growth is a key requirement to achieve population fitness and survival. Here we present a minimal, nucleotide-based coacervate model for active droplets, and report three key findings that make these droplets into evolvable protocells. First, we show that coacervate droplets form and grow by the fuel-driven synthesis of new coacervate material. Second, we find that these droplets do not undergo Ostwald ripening, which we attribute to the attractive electrostatic interactions and translational entropy within complex coacervates, active or passive. Finally, we show that the droplet growth rate reflects experimental conditions such as substrate, enzyme and protein concentration, and that a different droplet composition (addition of RNA) leads to altered growth rates and droplet fitness. These findings together make active coacervate droplets a powerful platform to mimic cellular growth at a single-droplet level, and to study fitness at a population level.
Selective hydroboration of unsaturated bonds by an easily accessible heterotopic cobalt catalyst
Authors: Li Chuhan , Song Shuo , Li Yuling , Xu Chang , Luo Qiquan , Guo Yinlong , Wang Xiaoming
Published: June 2021

Homogeneous earth-abundant metal catalysis based on well-defined molecular complexes has achieved great advance in synthetic methodologies. However, sophisticated ligand, hazardous activator and multistep synthesis starting from base metal salts are generally required for the generation of active molecular catalysts, which may hinder their broad application in large scale organic synthesis. Therefore, the development of metal cluster catalysts formed in situ from simple earth-abundant metal salts is of importance for the practical utilization of base metal resource, yet it is still in its infancy. Herein, a mixture of catalytic amounts of cobalt (II) iodide and potassium tert-butoxide is discovered to be highly active for selective hydroboration of vinylarenes and dihydroboration of nitriles, affording a good yield of diversified hydroboration products that without isolation can readily undergo further one pot transformations. It should be highlighted that the alkoxide-pinacolborane combination acts as an efficient activation strategy to activate cobalt (II) iodide for the generation of metastable heterotopic cobalt catalysts in situ, which is proposed to be catalytically active species.
A catalog of the diversity and ubiquity of bacterial microcompartments
Authors: Sutter Markus , Melnicki Matthew R. , Schulz Frederik , Woyke Tanja , Kerfeld Cheryl A.
Published: June 2021

Bacterial microcompartments (BMCs) are organelles that segregate segments of metabolic pathways which are incompatible with surrounding metabolism. BMCs consist of a selectively permeable shell, composed of three types of structurally conserved proteins, together with sequestered enzymes that vary among functionally distinct BMCs. Genes encoding shell proteins are typically clustered with those for the encapsulated enzymes. Here, we report that the number of identifiable BMC loci has increased twenty-fold since the last comprehensive census of 2014, and the number of distinct BMC types has doubled. The new BMC types expand the range of compartmentalized catalysis and suggest that there is more BMC biochemistry yet to be discovered. Our comprehensive catalog of BMCs provides a framework for their identification, correlation with bacterial niche adaptation, experimental characterization, and development of BMC-based nanoarchitectures for biomedical and bioengineering applications.
Giant multiple caloric effects in charge transition ferrimagnet
Authors: Kosugi Yoshihisa , Goto Masato , Tan Zhenhong , Kan Daisuke , Isobe Masahiko , Yoshii Kenji , Mizumaki Masaichiro , Fujita Asaya , Takagi Hidenori , Shimakawa Yuichi
Published: June 2021

Caloric effects of solids can provide us with innovative refrigeration systems more efficient and environment-friendly than the widely-used conventional vapor-compression cooling systems. Exploring novel caloric materials is challenging but critically important in developing future technologies. Here we discovered that the quadruple perovskite structure ferrimagnet BiCu3Cr4O12 shows large multiple caloric effects at the first-order charge transition occurring around 190 K. Large latent heat and the corresponding isothermal entropy change, 28.2 J K−1 kg−1, can be utilized by applying both magnetic fields (a magnetocaloric effect) and pressure (a barocaloric effect). Adiabatic temperature changes reach 3.9 K for the 50 kOe magnetic field and 4.8 K for the 4.9 kbar pressure, and thus highly efficient thermal controls are achieved in multiple ways.
Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation
Authors: Sakdapetsiri Chatsuda , Kaokhum Nitchakarn , Pinyakong Onruthai
Published: June 2021

Exiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal was achieved within 9 days when the starved state of immobilized cells was used. The oil removal activity of the immobilized cells was maintained in the presence of oil dispersant (89%) and at pH values of 7–9. Meanwhile, pH, oil concentration and salinity affected the oil removal efficacy. The immobilized cells could be reused for at least 5 cycles. The Arrhenius equation describing the relationship between the rate of reaction and temperature was validated as a useful model of the kinetics of retention of activity by an immobilized biocatalyst. It was estimated that the immobilized cells could be stored in a non-vacuum bag containing phosphate buffer (pH 7.0) at 30 °C for 39 days to retain the cells at 107 CFU g−1 carrier and more than 50% degradation activity. These results indicated the potential of using bio-cord-immobilized crude oil-degrading Exiguobacterium sp. AO-11 as a bioremediation product in a marine environment.