bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of the dynamics of anticonvulsant and anxiolytic action after oral administration of tin (II) chloride
Authors: Prokopchuk О. G. , Aleksandrova O. I. , Kravchenko I. A.
Published: September 2019

This article is devoted to the study of anticonvulsant and anxiolytic activity of tin (II) chloride by oral administration. The anxiolytic effect was established in models of the Open Field and Black-and-White Camera tests; the anticonvulsant activity of SnCl (II) was evaluated in model of acute generalized seizures with the determination of pentylenetetrazole (PTZ) minimum effective doses inducing clonic-tonic convulsions and tonic extension in test animals. Statistical indicators were calculated using the Microsoft Excel software package using the arithmetic mean value (M) and the mean deviation mean value (m). The obtained data show that tin (II) chloride is a promising compound for the prevention of anxiety states, as well as disorders containing the convulsive component. Three hours after the start of the experiment, the minimum doses of pentylenetetrazole for inducing clonic-tonic convulsions and tonic extension, exceeded the control group data by an average of 75%. A decrease in motor activity by half compared with the control in the open field test during the 5 hours of the experiment indicates the sedative effect of tin (II) chloride. Tin (II) chloride is a promising compound for the relief of convulsive states and correction of depressive disorders. Thus, it is of interest to further study the spectrum of its pharmacological activity in order the use it in medicine.
Evaluation of neuroprotective properties of Ademol with experimental traumatic brain injury conditions
Authors: Semenenko S. I. , Khodakivsky O. A. , Semenenko O. M. , Yakovleva O. O. , Semenenko N. O.
Published: September 2019

The choice of drugs in traumatic injuries of the head is one of the most complex problems in the complex treatment of such patients. The aim was to estimate the cerebroprotective action of Ademol on the indicator of lethality of rats against the background of an experimental traumatic brain injury (TBI). The experimental model of the TBI of severe severity was caused by the action of the flow of carbon dioxide under pressure that was created using a gas cylinder pneumatic gun. The therapeutic effect of Ademol on the model TBI was evaluated at doses of 1, 2 and 4 mg/kg intravenously. Pseudo-operative animals and control group received a 0.9% NaCl solution at a dose of 2 ml/kg. The results were processed using the statistical program StatPlus 2009. Used Fisher angular transformation when accounting for results in an alternative form. In the course of the experiment, it was found that in the control group at the end of the experiment, 8 days after the TBI model, death of 90% of the rats was recorded p<0.05. Ademol showed the highest protective effect on the brain at a dose of 2 mg/kg where the death of rats was 30% on the 8th day of the experiment. The obtained data indicate that among the studied doses 1; 2 and 4 mg/kg is conventionally effective at just 2 mg/kg, in which Ademol exhibited the highest neuroprotective activity (p<0.05).
Effect of angiolin on the functional state of the liver in experimental steatohepatitis
Authors: Pivtorak K. V.
Published: September 2019

For a real impact on the functional state of the liver in steatohepatitis, the appointment of metabolic correctors is suggested. The aim of the study is to evaluate the effect of the biologically active compound angiolin on markers of cytolysis and liver function in experimental nonalcoholic steatohepatitis. Experimental studies were performed on 110 white, non-linear, mature male rats weighing 180–200 g at the start of the experiment. A model of non-alcoholic steatohepatitis was created, rats were maintained for 8 weeks on a high-calorie, high-cholesterol diet. After creating the model some of the animals were transferred to a standard semi-synthetic starch-casein diet and administered for 4 weeks the biologically active compound Angiolin. The activity of enzyme markers of cytolysis — alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltranspeptidase (GGT) in serum was determined by standard methods. Liver biosynthetic function was assessed by the level of total protein and albumin. Statistical analysis of the obtained results was carried out with the use of the license program “STATISTICA 8” by Statsoft. After creating a model of non-alcoholic fatty liver disease in rats, an increase in body mass index occurred, high activity of ALT, AST, GGT was manifested and the concentration of total protein and albumin in the blood serum decreased compared to intact animals. An inverse correlation of mean strength was found between body mass index and total protein concentration as well as serum albumin. A strong inverse correlation between the value of ALT activity and the concentration of total protein and albumin in blood serum was established. The use of angiolin contributed to an increase in the concentration of total protein and albumin in the blood serum, an increase in the albumin / globulin coefficient, and a decrease in cytolysis. It was concluded that the biologically active compound angiolin positively affects the parameters of cytolysis and protein synthesis of the liver.
The role of nicotinamide in the correction of renal function in diabetic nephropathy
Authors: Yanitskaya L. V. , Osinskaya L. F. , Redko A. V.
Published: September 2019

Hyperglycemia of diabetes mellitus leads to the activation of the polyol way of oxidation of glucose with the activation of the enzymes of aldose reductase and sorbitol dehydrogenase and of their coenzymes NADPH and NAD, which triggers the mechanism of formation of sorbitol. The consequences of these changes lead to microangiopathy of the tissues of the kidneys, which may be one of the pathogenetic mechanisms of diabetic nephropathy. In an accessible literature, the role of coenzymes of sorbitol pathway in the development of diabetic nephropathy is not sufficiently defined. The purpose of the study was to study the content of NAD and NADPH coenzymes, their correlation, and their role in the mechanism of kidney damage in diabetes mellitus and to predict the possible correction of these changes with the NAD-nicotinamide derivative. The study was conducted on a model of streptotrozectinic diabetes mellitus (single administration of streptozotocin in a dose of 60 mg per 1 kg of body weight). Four weeks after induction of diabetes, nicotinamide (100 mg per 1 kg body weight) was injected. The level of glucose was determined by the Accu-chek (Roshe Diagnostics, Switzerland) glucose meter. The content of NAD and NADH was determined in the non-protein extracts. The statistical analysis was carried out using the Microsoft Excel statistical analysis program. The difference between the indicators was considered statistically significant (p<0.05). The NAD level was reduced by 31%, the NAD/NADN ratio was 32%. The dependence of the ratio of NADP/NADPN in conditions of hyperglycemia of diabetes mellitus with clinical manifestations of diabetic nephropathy is determined. A decrease in the ratio of NADP/NADPN to 38% in the rat kidney in the cortical layer was detected. The introduction of nicotinamide normalized the reduced content of NAD diabetic rats. These results provide perspectives for further research in which nicotinamide can be used as a renal protector.
Features of the prevalence of TLR-2 gene polymorphism (Arg753Gln) in patients with acute brucellosis with liver damage
Authors: Huseynov Elchin Mammed oglu
Published: September 2019

Brucella have the potential influence for all organs of the human body, which is reflected by a significant polymorphism of clinical manifestations, as a result, not always timely diagnosis of the disease and the development of complications that result of disability. The aim of our study was to determine the characteristics of the prevalence of polymorphism (Arg753Gln) of the TLR-2 gene in patients with acute brucellosis with liver damage. 178 patients with clinical symptoms of brucellosis were examined. According to the criteria for inclusion in a study of 178 patients, only 120 persons, who were the main group, fully met all criteria. All patients were tested for polymorphism (Arg753Gln) TLR-2. Statistical processing the results of the study was performed using the programs “SPSS 20.0”, “STATISTICA 6.0”. Among 120 patients with acute brucellosis, the disease of the hepatobiliary system had only 43 patients, which was 35.8% according to the laboratory and instrumental methods of investigation. The Gln/Gln genotype of the TLR-2 gene was determined in 3.3 times more in patients with acute liver brucellosis than in the patients without liver damage. Among the carriers of the genotype Gln/Gln, there is a significantly increased risk of brucellosis (χ2=17.52; p=0.0002; OR=4.81; 95% CI [0.98-23.59]), while carries of the homozygous genotype Arg/Arg on the contrary, has a protective effect on the development of brucellosis (OR=0.11, 95% CI [0.04 - 0.33]).
Prevalence of anemia in patients with ankylosing spondylitis and peculiarities of hemopoiesis and ferrokinetics, depending on the degree of anemia
Authors: Zviahina O. V. , Shevchuk S. V. , Shevchuk O. V.
Published: September 2019

Anemic syndrome is a frequent complication of ankylosing spondylitis (AS), worsening the course of the disease and prognosis. The purpose of this work was to determine the frequency of anemia, relationship with age, sex and duration of the disease, as well as the peculiarities of hemopoiesis and ferrokinetics in patients with AS. 118 patients with AS and 26 controls were examined. Hematologic parameters were determined on the apparatus of ERMA PCE-210 (Japan), indicators of ferrokinetics were determined on a biochemical analyzer Humalyzer 2000 using sets in accordance with the instructions of the manufacturer. Statistical data was processed using the Microsoft Office Excel statistical software package. The probability of the results was estimated using Student's criteria (probable results were considered at p<0.05) and Fischer's criterion. Analysis of data showed that anemia was detected in 28.8% of patients. In 79.4%, anemia was mild, and 20.6% had an average degree of severity. Anemia was detected in 68.8% of women and 22.5% of men. Anemia was observed in 52.9% of patients with a disease duration of up to 5 years. According to cytometric indices, it is normocytic anemia in 55.9%, microcytic it is in 26.5%, and only in 17.6% is macrocytic. In patients with moderate severity of anemia, serum iron levels, ferritin and coefficient of saturation of transferrin were significantly lower, compared with light, and total iron binding capacity (TIBC) and sTfR levels were higher, p<0.05. Thus, among patients with SAR, anemia is detected in 28.8% of patients with predominantly mild degrees. Anemia is more often diagnosed in women than in men. There is no relationship between age and the occurrence of anemia. In debut disease, every second patient has anemic syndrome. According to the cytometric characteristic, anemia is a mild degree of normocytic, and anemia of middle degree is microcytic. Patients with microcytic anemia had the lowest levels of iron, ferritin, coefficient of saturation of transferrin, and the highest levels of TIBC and sTfR.
Comparison of the effectiveness of caudal and transforaminal epidural block in the patients with low back pain syndrome due to degenerative-dystrophic changes of spine
Authors: Moskovko G. S. , Nikitchuk Ya. V. , Kostiuchenko A. V. , Tytarenko N. V. , Semenenko A. I.
Published: September 2019

The study included 258 (96 males, 162 females) of patients in the age of 52.2±14.16 years with pain in the lumbar-sacral part of spine due to degenerative-dystrophic changes. Among them: 30 patients received medication treatment (group 1, control), 114 transforaminal epidural block (group 2) and 114 — caudal epidural blockades (group 3, n = 114). Period of observation was 6 months. The assessment of the intensity of the pain was performed on the visual analog scale (VAS), the quality of life — on the Oswestry Disability Index (ODI) and the Short Form Health Survey-36 (SF-36). Caudal and transforaminal epidural blockades could effectively reduce the intensity of pain (p<0.05). 98.2% of patients reported regression of pain immediately after caudal epidural blockades, but the positive result remained during 6 months after procedure in only 59.6% of patients. Transforaminal epidural block contributed to a significant decrease the intensity of pain according to the VAS in the whole group and different monitoring periods in 76.4–91.2% of patients. Both methods are effective for treatment of the lumbar pain syndrome and are associated with a positive dynamic of patient quality of life. However, caudal epidural blockades are more effective in the short term.
Dynamics of clinical characteristics and biomarkers of coagulation hemostasis within the application of the endovenous electric welding for varicose disease of the lower extremities
Authors: Savoliuk S. I. , Glagolieva A. Yu.
Published: September 2019

The article presents the results of prospective longitudinal study, in which the dynamics of fibrinogen, D-dimer, the platelet count levels have been evaluated in comparison with clinical changes within the application of endovenous electric welding (EVEW) in automatic mode for varicose vein treatment in 33 patients. The statistical analysis of the results was performed using SPSS Inc. software. (Chicago, IL, USA, version 22.0). One week after the operation, a complete obliteration of the great saphenous vein, as determined by ultrasonography, was observed in 26 patients (78.8%), while in 7 patients, a remaining lumen was registered (21.2%); after 12 months, the complete occlusion of the trunk without signs of recanalization or varicose disease progression was determined in 100% patients. After 1 week, the symptoms of venous stasis (edema, heaviness in the lower extremities) disappeared in 32 (97%) patients; in one case, the edema remained until the day 12. The great saphenous vein occlusion after EVEW application was accompanied by changes in coagulation homeostasis, which reflected in the dynamics of D-dimer, fibrinogen and the platelet count. One week after surgery, these parameters were higher in patients with incomplete occlusion, indicating the maintenance of coagulation activity of the body as a component of treatment aimed to complete the process of insufficient vein obliteration. Gradual decrease in platelet count as one of the markers of cell apoptosis indicates a continuous decrease in the activity of venous wall inflammation. No blood flow impairment in the deep veins of the lower extremities or thrombophlebitic changes in the trunk of the great saphenous vein or its tributaries have been detected.
Frequency and nature of complications and hemostasis indicators in patients with ishemic artery disease, who had undergone percutaneous coronary angioplasty (based on the 18-month clinical observation)
Authors: Storozhuk N. V. , Dovgalyuk T. V. , Storozhuk B. H.
Published: September 2019

Determination of the nature, frequency and causes of restenosis/thrombosis in patients with ishemic artery disease and PKA in prospective observation is significant for the development of preventive measures depending on the clinical (comorbidity) and biochemical (state of the hemostasis system) characteristics of each patient. The goal is to study the frequency and nature of complications in patients with coronary artery disease and PKA and their hemostasis indicators. An 18-month follow-up (outpatient, inpatient and telephone mode) was performed for 91 patients, of whom 32 had a late restenosis/stent thrombosis in history (more than 6 months after stenting) and 59 – who did not have restenosis. All patients at the beginning of the study once determined the main indicators of hemostasis (soluble fibrin, D-dimer, fibrinogen and protein C). Patients followed by clinical observation did not receive anticoagulant treatment and received dual antiplatelet therapy. Statistical processing was performed using the methods of variation statistics and correlation analysis. The results of the study revealed that in the group of patients with restenosis and diabetes mellitus in the history, the risk of developing restenosis/thrombosis of the stent is twice as high as in the general group. Herewith, damage to other parts of the cardiovascular system is practically not excluded with the same frequency. The results of the study also revealed significant abnormalities in the system of hemostasis in patients with the presence of both restenosis/thrombosis and other vascular lesions. Thus, in patients with thrombotic complications, there is a sharp increase in the factor of prethrombosis – soluble fibrin, with a clear depression of the fibrinolytic linkage of hemostasis, as indicated by the low level of D-dimer. In addition, these violations of the sF/D-d ratio are associated with inhibition of the anticoagulant ability of hemostasis represented by the protein C. Consequently, the course of the disease in patients with IAD and PKA is closely related to the presence of comorbid states and hemostasis
Predictors of unfavorable course of acute myocarditis with reduced left ventricular ejection fraction
Authors: Cherniuk S. V.
Published: September 2019

Early prediction of myocarditis clinical course still remains one of the actual tasks of cardiological science, which has a significant practical value. The purpose of the study was to determine the predictors of unfavorable course of myocarditis with reduced ejection fraction (EF) of the left ventricle (LV) on the basis of follow-up during 24 months. The study included 90 patients with acute myocarditis and reduced LV EF, who were examined in the first month after the disease onset, after 6, 12 and 24 months of follow-up. All patients underwent for echocardiography, Holter monitoring of the electrocardiogram and cardiac magnetic resonance imaging. Statistical processing of the results was performed using the Excel XP software (Місrosoft Office, USA) and Statistica for Windows v. 6.0 (Statsoft, USA). It was found that restoration of cardiac contractility in patients with myocarditis and reduced LV EF began with an increase in the index of LV longitudinal global systolic strain, which occurred 6 months after the debut of the disease and was associated with a decrease in the number of LV segments affected by the inflammatory process, and a significant increase of LV EF and a decrease of LV end-diastolic volume were observed only 1 year after the disease onset. The predictors of cardiovascular events in patients with acute myocarditis during the next 24 months from the onset of the disease, were detected in the 1st month from the debut of the disease and included following pathological changes: value of the LV EF ≤ 30%; reduction of LV longitudinal global systolic strain ≤ 7.0%; evidence of non-sustained ventricular tachycardia, presence of inflammatory changes in ≥6 LV segments, presence of delayed enhancement in ≥ 5 LV segments. Prospects for further research are the creation of registries of patients with myocarditis on the basis of multicenter clinical trial results on a large number of patients surveyed in order to improve diagnosis, prediction of clinical course and identification of early diagnostic markers of cardiovascular events and quantitative risk assessment of their development.
Clinical and laboratory features of viral hepatitis B among HIV-patients in Zambia
Authors: Moroz L. V. , Soni S. Ch. , Chichirelo-Konstantynovych K. D. , Kulias S. M. , Popovych O. O.
Published: September 2019

Viral hepatitis B course in HIV patients in Zambia is still bad studied. At present, the study of the influence of immunosuppression on the clinical and laboratory features of viral hepatitis B acquires a special relevance. The aim of research is to investigate clinical and laboratory features of viral hepatitis B course among HIV-patients in Zambia. Epidemiological, descriptive, serological (HBV, HIV markers), biochemical (cytolysis and cholestasis syndromes’ indicators), statistic method for determining the reliability using average errors (m), t-criterion of the probability difference of the Student) methods are used. The results show HBV/HIV-positive Zambian patients reliably differ from HBV-monoinfected ones. As well, HBV/HIV-positive patients have significantly higher indexes of asteno-vegetatic, dyspeptic syndromes, hepato- and splenomegaly, cytolysis indexes (alanine aminotransferase, lactate dehydrogenase), which are associated with increase of HIV-infection’s stage. Hepatocellular deficiency’s indicators don’t differ reliably in comparison groups. Therefore, HBV/HIV-infected patients have more severe clinical and laboratory course than HBV-monoinfected ones.
Medical treatment of hernia of abdomen wall with the use of different plastic materials
Authors: Khimich S. D. , Zheliba M. D. , Reva A. I. , Funikov A. V. , Prevar A. P. , Formanchuk A. M. , Katelian O. V. , Rautskis V. P.
Published: September 2019

The purpose of the work is to analyze the results of surgical treatment of hernias using different methods of hernioplasty. The results of treatment of 176 patients with abdominal hernias, which were monitored from 1995 to 2008, were analyzed. Patients were divided into 2 groups. The first group (85 patients) included patients who underwent surgical treatment with autodermal plasty. Second group (91 patients) consisted of patients who underwent hernioplasty using mesh allografts. All patients were comprehensively examined. In obese patients, an antimicrobial film was used to glue the skin under the mammary glands and “folds of the abdomen” to prevent microbial contamination of the wound. All operated patients had a normal postoperative condition, and the postoperative period in both groups was almost the same. We did not observe complications from the side of the postoperative wound in patients of the 1st group. The wounds healed by primary intention. All patients were discharged on the 8–10 day in satisfactory condition. 2 relapses were noted: in one patient 1 month after surgery, in the second — 10 years after surgery. In the 2nd group, suppuration of the postoperative wound was observed in a patient with a II degree of obesity. The average number of bed-days in the 2nd group was approximately the same as in the 1st group. The number of bed-days statistically significantly increased with an increase in the degree of obesity in these patients. Thus, the use of mesh allografts is an effective method of treating hernias and they should be used strictly according to indications. Autodermal plastic surgery using a skin flap is also a reliable surgical method for the treatment of hernias. A significant difference in the indices in the treatment of patients of both groups (with the exception of the cost of mesh allografts) was not found.
Results of observation of the postoperative period in patients with surgical aortic pathology after surgical intervention using cardiopulmonary bypass
Authors: Khyzhniak K. A. , Volkova Y. V. , Sharlai K. Y. , Khartanovych M. V.
Published: September 2019

The aim of the work was to analyze the results of the observation of the postoperative period in patients with surgical aortic pathology after surgical intervention using cardiopulmonary bypass. 118 patients with surgical aortic pathology (SAP) were examined. Patients were divided into 2 groups. Group I included 46 patients who were additionally prescribed a solution of meglumin sodium succinate (reamberin), group II included 46 patients who were additionally assigned a solution of D-fructose-1,6-diphosphate sodium hydrate salt (esophosphine). The control group (K) consisted of 26 patients who had surgical pathology of the aorta, all the criteria coincided with patients of groups I and II, but did not have additional substances as part of intensive care (IT) in order to prevent postoperative cognitive dysfunction (PСD). Analyzed the patient's condition on a scale of postoperative mortality prediction EuroSCORE II, the depth of anesthesia with a BIS monitor, the qualitative composition of the leukocyte formula, nasal and rectal temperature, blood gas composition, electrolyte level, glucose, lactate, coagulogram indices, hemolysis and phosphates; levels of autoantibodies to brain antigens (MBP, calcium binding protein S-100, NSE and GBA) were determined in the serum by ELISA a day before the operation and on the first, third, seventh and fourteenth day after surgery. The morphometric and functional properties of erythrocytes were investigated the day before the operation (starting level), 12:00 after the operation, and on the third day of hospital stay. The initial assessment of the cognitive abilities of the patients was made the day before the operation, on the third, seventh and fourteenth day of hospital stay. Used to determine the magnitude of the coefficient of linear Pearson correlation. In the analysis, no significant differences were found between the preliminary data on the phosphorus content in the patients' blood, however, the recovery dynamics of its numbers differed in the K, I and II patients. So, in group K and in group I, after 12:00 after surgery, the level of phosphatemia was 0.86 ± 0.21 mmol/l and 0.85 ± 0.18 mmol / l, on the 3rd day of hospital stay — 0,94 ± 0.08 mmol/l and 0.97 ± 0.04 mmol/l, on the 7th day — 1.04 ± 0.16 mmol/l and 1.07 ± 0.21 mmol/l and on The 14th day — 1.08 ± 0.12 mmol/l and 1.1 ± 0.14 mmol/l. It can be noted that the dynamics of blood phosphatemia in patients of groups K and I was identical, its figures almost coincided in terms of the level of phosphorus in the blood and the corresponding number of patients in the variation rows of patients in both groups. It may be noted that there is an unconditional positive effect on the course of the perioperative period of the option of infusion therapy in the IT complex in patients of group II.
The effect of hormonal disbalance on the aging rate change in the patients with arterial hypertension and comorbid endocrinopathies
Authors: Nemtsova V. D. , Ilchenko I. A. , Zlatkina V. V.
Published: September 2019

Due to the growing number of patients with age-related diseases, the aim of the study was to investigate in the changes of aging rate (AR) in patients with comorbid course of arterial hypertension (H), type 2 diabetes mellitus (T2DM) and subclinical hypothyroidism (SH) and to study the features of these changes depending on hormonal imbalance. 118 patients (63 women and 55 men, average age — 53.6±4.3 years) were divided into 3 groups: group 1 (n=37) with isolated H; group 2 (n=42) — with H and T2DM; group 3 (n=39) — with H, T2DM and SH. The investigation program included: measurement of anthropometric parameters (blood pressure, height, body weight (BW), body mass index (BMI)), carbohydrate and thyroid metabolism using standard methods, biological age (BA) by V.P. Voitenko et al. Statistical processing was performed using the Statistica for Windows 8.0 software package. When evaluating AR, physiological aging was found in 8 patients (21.6%) of group 1, in 4 (9.5%) patients of group 2 and 3 (7.7%) of patients in group 3. In the overwhelming majority of the examined patients, premature aging (PA) was noted, however, the acceleration of PA between patients of groups 2 and 3 was not differ significantly (p>0.05). The increase in AR in group 2 patients was accompanied by an increase in BA by 7.2 years, in 3 group patients — by 7.3 years compared with their chronological age. A correlation analysis revealed a positive relationship between BMI and coefficient of aging rate (CAR) (r=0.679; p<0.05); BMI and BA (r=0.562; p<0.05) and CAR and the TSH level (r=0.050; p=0.388) in the 3rd group. Thus, the presence of hypertension and comorbid endocrinopathies — T2DM and SH significantly increases the AR and when assessing the effect of endocrine disorders, the presence of T2DM is more important than SH.
New functions and tasks of the teacher of the modern higher medical school in the preparation of qualified specialists
Authors: Denysiuk О. М. , Voloshchuk N. I. , Saienko А. V. , Pashynska О. S. , Melnyk A. V.
Published: September 2019

The main activity orientations of instructor of higher medical educational institution in preparation of highly-skilled doctors as well as self-professional development (educational-methodical, scientific-research, social, educational) are outlined in the article. The necessity of introducing new active, interactive and informational-communication methods of education in conditions of “anthropocentric” model of education is substantiated. The roles and functions of modern instructor (preceptor, trainer, provider, mentor, coach, facilitator, assessor) in providing the conditions for obtained knowledge, competence and skills of students are disclosed in the article. It is shown that among the important functions of the teacher of the higher medical school is the formation of practical orientation and foreign language competences of the future doctor.
Ways of optimization of the teaching of the subject “Pathophysiology” and preparation of future doctors on the basis of National Pirogov Memorial Medical University, Vinnytsya
Authors: Rikalo N. A. , Piliponova V. V.
Published: September 2019

The main principles of organization of educational process on the Department of Pathophysiology of National Pirogov Memorial Medical University, Vinnytsya are highlighted. In order to improve the quality of the training of future doctors, along with standard teaching methods such as lectures, practical classroom and independent out-of-class studies, alternative methods such as conducting brain-rings are used to stimulate a creative approach to the study of discipline. This allows to improve the quality of students' training in accordance with European standards, to formulate a clinical thinking and creative approach.
Modern possibilities of application of the project method while organizing the educational process at the postgraduate level
Authors: Savoljuk S. I. , Chodos V. A. , Lusenko V. N. , Zavertilenko D. S.
Published: September 2019

The purpose of the work is to optimize the principles and methods of organizing independent work on the basis of interactive learning technologies (design method) in the context of planning the educational process of interns in the specialty “Surgery”, clinical residents, postgraduates and students (optional) in the cycles of thematic improvement within the program Cycles of the Chair of Surgery and Vascular Surgery of Shupyk National Medical Academy of Postgraduate Education. The use of traditional educational and pedagogical means, technologies and methods does not ensure the intensive development of all areas of activity at the postgraduate level. Overcoming these contradictions is possible provided that new modern and innovative approaches to the formation of knowledge and skills are taken into account, taking into account the existing possibilities of information and communication technologies. Solving the problem is possible through the use of interactive learning methods — the method of projects. The introduction of interactive methods in the educational process at all levels of postgraduate training is aimed at the systematic formation of knowledge of specialists who can solve problems in various areas of practical professional activities.
Methodical aspects of teaching sports medicine at the medical faculty of National Pirogov Memorial Medical University, Vinnytsya
Authors: Sarafinyuk L. A. , Kyrychenko Yu. V. , Kyrychenko V. I. , Stefanenko I. S.
Published: September 2019

In the modern educational space, the important task is to bring the current educational sport education program to European standards of education to improve the quality of training future medical doctors. The purpose of the work is to synthesize the methodological aspects of the subject “Sport Medicine” and the peculiarities of controlling the knowledge of the students of the 4th year of the medical faculty of National Pirogov Memorial Medical University, Vinnytsya. The educational process at the department is carried out according to the new curriculum by training specialists of educational qualification level “Specialist” in higher educational institutions of the Ministry of Health of Ukraine. Its main direction is strengthening of the motivation of studying the discipline “Sport medicine” and the formation of clinical thinking of medical students by the faculty and professors of the department. This is ensured by highlighting the professional orientation of the information given in practical classes and lectures, the constant updating of the program material by scientific facts, obtained through modern innovative technologies. Achievement of the set goal is due to the research work of the department staff. Methodical materials for students of the medical faculty have been created, in which a lot of attention is paid to the practical direction in future professional activities. The final assessment received by students after the completion of the study of this discipline consists of the average score for current progress, the evaluation of the results obtained for the registration of the medical-control card of the athlete and the athlete (form 061 and form 062) and the score obtained on the differentiated score. Department of physical education and exercise therapy National Pirogov Memorial Medical University, Vinnytsya is working on optimizing the educational process in order to prepare qualified doctors. The teaching staff from year to year updates the program material, which provides a high practical and theoretical level of our medical students.
Clinical features of bronchial asthma course in children of Vinnytska oblast, depending on age and level of the disease control
Authors: Zhmurchuk V. M.
Published: September 2019

According to various epidemiological studies, the prevalence of bronchial asthma (BA) in children in Ukraine ranges from 5 to 12%. Despite numerous studies of etiological factors, mechanisms of pathogenesis, the creation of international and national programs, until now it is not possible to take control of the morbidity and course of asthma. Therefore, the purpose of our study is to determine the clinical features of the course of bronchial asthma in children, depending on the age and level of disease control. We examined 227 children with asthma aged 6 to 17 years. In the objective examination, respiratory failure, shortness of breath, dry cough, nasal breathing difficulties, wheezy breathing, emphysema, bloating of the chest, were taken into account. The statistical processing of the results was carried out using the IBM SPSS Statistics, version 20 (2013), using parametric and non-parametric methods for evaluating the results. Under supervision were 181 boys (79.73±0.5%), from the total number of examined patients with asthma and 46 girls (20.27±1.98%). The control group included 40 practically healthy children aged 6 to 17 years. It was established that the persistent course of light and medium severity was noted 2.5 times more often than the severe course of asthma, and also 2.4 and 2 times more often (p≤0.01) in the age group of 6–11 years, unlike children 12–17 years old. Severe exacerbations were twice as likely to occur in patients with asthma-mediated etiology, and lungs — in patients with atopic asthma. Uncontrolled levels of the disease were noted 1.6–2 times more often in non-atopic form of asthma, than in its other variants. Thus, among children with asthma, mixed asthma and non-atopic asthma with an uncontrolled course, there is a clear need to find factors that are trigger in the development of exacerbations of the disease, determine their duration and severity, which helps to prevent the progression of asthma and avoid complications.
Ultraviolet bactericidal irradiation of the air of classrooms for junior lyceum-students and its efficiency
Authors: Redchits M. A. , Serheta I. V. , Redchits Ye. M.
Published: September 2019

Literature data and materials of their own research indicate that there are significant deficiencies in the air environment of the closed educational facilities of modern schools of lyceums, which is the reason for the high level of acute respiratory illness among younger pupils in the autumn-winter period and requires the introduction of a set of measures for its optimization. The aim of the study was to provide scientific substantiation of methodological approaches to the development and arrangement of stationary ultraviolet units and to control the quality of bactericidal irradiation of the air environment of educational facilities of junior high school pupils, to determine the degree of effectiveness of its impact on the state of the air environment and the level of morbidity of students with temporary disability. Hygienic, light engineering, microbiological and statistical methods of research were used in the work. Statistical processing of the data was carried out using generally accepted methods. In order to improve the air environment of the educational facilities of junior high school students, their wide aeration was used in the spring and autumn periods of the year. For the first time a system of stationary irradiators with artificial sources of bactericidal ultraviolet radiation by BUB-30 lamps was developed and implemented directly in the training rooms used during the autumn-winter period during breaks after the first and last lessons. The results of the measures showed positive changes on the part of the leading correlates of the microclimate of the air environment. Total sowing of air by microorganisms improved in all seasons of the year, and during the winter period decreased by 1.8 times. Selected as a criterion for assessing the effectiveness of airspace optimization in educational facilities, the level of morbidity with temporary disability of schoolchildren during third year (р˂0.001) determined the presence of dynamic shifts of favorable content, indicating an increase in the level of adaptive resources of the organism of younger schoolchildren-lyceum pupils.
Modern views on etiopathogenesis of traumatic injuries of the lower jaw against the background of osteoporosis and the use of drugs for correction of the processes of reparative osteogenesis
Authors: Lykhytskyi О. О.
Published: September 2019

The purpose of the work is to analyze contemporary views on the etiopathogenesis of traumatic injuries of the lower jaw against the background of osteoporosis and the use of drugs to correct the processes of reparative osteogenesis. The analysis is based on a review of domestic and foreign articles and studies for 2006–2018, using the scientometric databases PubMed, eLIBRARY.RU, Web of Science. Damage to the lower jaw, namely its fractures, is a fairly common pathology both in Ukraine and in other countries of the world, and is associated with a violation of many key functions, in particular, nutrition and communication, and in case of complications, even a violation of aesthetic function. One of the factors that increase the likelihood of fracture formation is osteoporosis, a systemic disease of bone tissue characterized by a decrease in bone mass per unit volume and a violation of its microarchitecture. And if the problem of fractures of the lower jaw is sufficiently disclosed, then the features of the formation of fractures of this bone against the background of diseases, including against the background of osteoporosis, are not adequately addressed in both Ukrainian and foreign sources. This article presents modern works on the etiology and pathogenesis of traumatic injuries of the lower jaw against the background of osteoporosis and modern methods of correction of reparative osteogenesis.
Does every family couple that being treated with assisted reproductive technologies need to use preimplantation genetic testing for aneuploidy (PGT-A)?
Authors: Lyovkina O. L. , Derii S. S. , Kuzmenko Y. R.
Published: September 2019

The increase in the frequency of using assisted reproductive technologies (ART) dictates the need for pre-implantation diagnosis of embryos to determine which of them are euploid and recommended for embryo transfer (ET), since the main cause of implantation failures when using in vitro fertilization (IVF) is ET aneuploid or mosaic ETs or mosaic imitations that use in vitro fertilization (IVF) is ET aneuploid or mosaic imitative failures when using in vitro fertilization (IVF) is ET aneuploid or mosaic imitations. . For this purpose, the PGT-A technology was created, since the appearance of which and its further development there have been discussions about the feasibility of using PGT-A for each pair, which turned to the use of ART. The purpose of this literature review is to describe the current status of PGT-A and determine the prospects for its widespread adoption in the practice of reproductive medicine. A literature search was carried out in the PubMed and Cochrane databases for the last 10 years. An analysis of the literature has shown that IVF with PGT-A technology has significant advantages over traditional IVF; The technology has a number of technical and financial limitations, which makes it difficult to massively introduce technology into the practice of reproductive medicine, therefore at this stage there should be clear indications for using IVF with PGT-A.
Significance of aldosterone synthase gene (CYP11B2) polymorphism as a component of the renin-angiotensin-aldosterone system in the pathogenesis of myocardial hypertrophy in hypertension: literature review and own experience
Authors: Lozinska M. S. , Zhebel V. M.
Published: September 2019

Aldosterone synthase gene is one of the candidate genes responsible for the effects of aldosterone. In particular, there are some evidences concerning the influence of this gene on the myocardial remodeling in hypertension. The analysis and summary of the results of published researches devoted to the study of aldosterone synthase gene (CYP11B2) polymorphism and its importance in the processes of hypertensive myocardial remodeling are presented in the article. Pub Med and EMBASE databases were used. The results of the study of CYP11B2 polymorphism within the framework of the research work at the Department of Internal Medicine of Medical Faculty №2 are presented in the article. The aim of the study is to study the importance of polymorphism of aldosterone synthase gene in the pathogenesis and clinic of hypertension. It has been shown that among both, hypertensive and males, residents of Podillia region of Ukraine, the overwhelming majority were carriers of TC polymorphism of CYP11B2 gene. These data are in the agreement with the previous studies in the European population.
Calibration of Traffic Incident Simulation Models Using Field Data
Authors: Rompis Semuel Y. R. , Habtemichael Filmon G ,   Department of Civil Engineering, Sam Ratulangi University, Indonesia,   National Center for Sustainable Transportation Technology, Indonesia
Published: September 2019

This study presents a methodology to calibrate a traffic incident simulation model, particularly in a freeway. The queue length was used as the objective of the simulation model calibration in this study. The simulation model was set up using Traffic Simulation Model PTV. VISSIM. Multiple incident durations were simulated, and the generated queue lengths were compared to the observed queue lengths. The observed queue lengths were estimated using the LWR model and shockwave speeds calculated using the field data. The results confirm that calibrated VISSIM incident model can signify the shockwave propagation speeds and queue length in the event of freeway incident. Such a model can be implemented as an instrument for setting up traffic management strategies to alleviate the incident’s impact.
Mohs Micrographic Surgery Sine Microscopy: Is mass spectrometry an upcoming intraoperative cancer margin assessment tool?
Authors: Siu-Yung Chan Lawrence
Published: September 2019

A special kind of skin cancer surgery, named Mohs micrographic surgery (or Mohs surgery), was invented by a University of Wisconsin Otolaryngologist, Dr. Frederick Mohs [1]. The initial Mohs surgery procedure, also called chemosurgery, employed a zinc chloride-containing chemical paste to destroy cancerous tissue layer by layer under microscopic control [1]. The procedure was subsequently modified to the current and well-accepted form, where patients affected by non-melanoma skin cancers of certain facial locations, recurrent skin cancers, or skin cancers with certain aggressive histologic features would be treated by a layered excisional procedure without the pain associated with zinc chloride paste [2]. The current procedure, with the ability to determine cancer margin in an intraoperative fashion, utilizes microscopic examinations of stained frozen sections obtained from an excised layer of orientation-marked skin where the clinical cancer is visually observed, while the patient waits for the result. Microscopic identification of cancer at the margin of excised skin layer will necessitate the return of the patient to the surgical table for addition layer of excision [3]. The process repeats if needed, until all margins are clear of skin cancer, at which point the Mohs surgeon will close the wound defect, commonly utilizing adjacent tissue transfer technique called flap [4].
Application of A Simple and Reliable Cell Proliferation and Viability Assay in Green Tea Extract-and Catechin-Induced Growth Inhibition of Cancer Cells
Authors: Hu Xiaotang
Published: September 2019

Several colorimetric cell proliferation/viability assays have been widely used in basic research in recent years. Meanwhile, the limitations of these methods in some applications have been reported. We found a significant drawback when these common methods were used to evaluate the effect of green tea extract or catechins on cell proliferation and viability. To overcome this problem, a simple and reliable method was developed for measuring cell proliferation/viability in response to green tea extract or catechins. This method is based on the principle that the color intensity caused by green tea extract or catechins is proportionally correlated to the cell inhibition and cell death. This method is simple, reliable, and economic. By using this method, we demonstrated that the green tea extracts and catechins tested significantly inhibited both suspension leukemia and adherent epithelial cancer cells, which suggest that the green tea extract and its catechins are a potential cancer prevention and chemotherapy agent. It appears that multiple mechanisms are responsible for the green tea extract- and catechin-induced growth inhibition of the cells.
Appendicitis Mimicking Orchitis-A Case Report
Authors: RJ NG Bertrand , Pradeep Subramanian
Published: September 2019

Acute appendicitis is one of the most common emergency surgical procedure, yet atypical presentation sometimes can be challenging for clinician. I present a case of 19-year-old gentleman that initially presented with 1day history of bilateral testicular pain and lower abdominal pain. His past history includes a positive sexual history. Initial ultrasound of the testis showed bilateral orchitis and an equivocal appendix. With a significantly raised inflammatory marker and highly suspicious for appendicitis, a CT scan was obtained which showed perforated appendicitis and the patient underwent laparoscopic appendicectomy with resolution of symptoms after that. We encourage clinician to be aware of this clinical pitfall as patient can sometimes be managed in other department to minimise any delayed diagnosis or any unnecessary procedure.