bluuebunny/crossref_metadata_2025 huggingface dataset:
Direct Observation of Hillslope Scale StorAge Selection Functions in Experimental Hydrologic Systems: Geomorphologic Structure and Preferential Discharge of Old Water
Authors: Kim Minseok , Volkmann Till H. M. , Wang Yadi , Meira Neto Antonio A. , Matos Katarena , Harman Ciaran J. , Troch Peter A.
Published: January 2022

Spatially integrated water transport dynamics at the hillslope scale have rarely been observed directly, and underlying physical mechanisms of those dynamics are poorly understood. We present time‐variable transit time distributions and StorAge Selection (SAS) functions for a 28 days tracer experiment conducted at the Landscape Evolution Observatory, Biosphere 2, the University of Arizona, AZ, USA. The observed form of the SAS functions is concave, meaning that older water in the hillslope was preferentially discharged than younger water. The concavity is, in part, explained by the relative importance of advective and diffusive water dynamics and by the geomorphologic structure of the hillslopes. A simple numerical examination illustrates that, for straight plan‐shaped hillslopes, the saturated zone SAS function is concave when the hillslope Péclet (Pe) number is large (and thus when the advective water dynamics are more pronounced). We also investigated the effect of hillslope planform geometry on the saturated zone SAS function using a model and found that the more convergent the plan shape is, the more concave the SAS function is. A numerical examination indicates that the unsaturated zone SAS function is concave for straight and convergent hillslopes when the soil thickness is uniform. The concavity of those subcomponent SAS functions signifies that the hillslope scale SAS function is concave for straight or convergent plan shape hillslopes when the hillslope Pe number is high.
Geochemical Records Reveal Protracted and Differential Marine Redox Change Associated With Late Ordovician Climate and Mass Extinctions
Authors: Kozik Nevin P. , Gill Benjamin C. , Owens Jeremy D. , Lyons Timothy W. , Young Seth A.
Published: January 2022

The Ordovician (Hirnantian; 445 Ma) hosts the second most severe mass extinction in Earth history, coinciding with Gondwanan glaciation and increased geochemical evidence for marine anoxia. It remains unclear whether cooling, expanded oxygen deficiency, or a combination drove the Late Ordovician Mass Extinction (LOME). Here, we present combined iodine and sulfur isotope geochemical data from three globally distributed carbonate successions to constrain changes in local and global marine redox conditions. Iodine records suggest locally anoxic conditions were potentially pervasive on shallow carbonate shelves, while sulfur isotopes suggest a reduction in global euxinic (anoxic and sulfidic) conditions. Late Katian sulfate‐sulfur isotope data show a large negative excursion that initiated during elevated sea level and continued through peak Hirnantian glaciation. Geochemical box modeling suggests a combination of decreasing pyrite burial and increasing weathering are required to drive the observed negative excursion suggesting a ∼3% decrease of global seafloor euxinia during the Late Ordovician. The sulfur datasets provide further evidence that this trend was followed by increases in euxinia which coincided with eustatic sea‐level rise during subsequent deglaciation in the late Hirnantian. A persistence of shelf anoxia against a backdrop of waning then waxing global euxinia was linked to the two LOME pulses. These results place important constraints on local and global marine redox conditions throughout the Late Ordovician and suggest that non‐sulfidic shelfal anoxia—along with glacioeustatic sea level and climatic cooling—were important environmental stressors that worsened conditions for marine fauna, resulting in the second‐largest mass extinction in Earth history and the only example during an icehouse climate.
Visualizing the Distribution of Water in Nominally Anhydrous Minerals at the Atomic Scale: Insights From Atom Probe Tomography on Fayalite
Authors: Liu Jia , Taylor Sandra D. , Qafoku Odeta , Arey Bruce W. , Colby Robert , Eaton Arielle , Bartrand Jonah , Shutthanandan Vaithiyalingam , Manandhar Sandeep , Perea Daniel E.
Published: January 2022

Nominally anhydrous minerals (NAM) such as olivine are important reservoirs for water in the Earth’s upper mantle, although the association of water with crystallographic defects is poorly understood. Here, hydrated regions at the atomic scale in fayalite (Fe2SiO4) are directly resolved using atom probe tomography. Site‐specific analyses reveal regions that are either compositionally homogenous or contain nanometer‐long channels that are consistent with hydrated defects. Na as a minor element is also enriched within the interlayers, indicating the formation environment is associated with Na geochemically. The ability to directly visualize and characterize hydrated regions at the nanometer scale can provide critical insight into the material properties of NAM and their impact on planetary‐scale processes, such as the influence of water‐bearing minerals on mantle rheology and geodynamics.
Parsing the Kinetic Energy Budget of the Ocean Surface Mixed Layer
Authors: Zippel Seth F. , Farrar J. Thomas , Zappa Christopher J. , Plueddemann Albert J.
Published: January 2022

The total rate of work done on the ocean by the wind is of considerable interest for understanding global energy balances, as the energy from the wind drives ocean currents, grows surface waves, and forces vertical mixing. A large but unknown fraction of this atmospheric energy is dissipated by turbulence in the upper ocean. The focus of this work is twofold. First, we describe a framework for evaluating the vertically integrated turbulent kinetic energy (TKE) equation using measurable quantities from a surface mooring, showing the connection to the atmospheric, mean oceanic, and wave energy. Second, we use this framework to evaluate turbulent energetics in the mixed layer using 10 months of mooring data. This evaluation is made possible by recent advances in estimating TKE dissipation rates from long‐enduring moorings. We find that surface fluxes are balanced by TKE dissipation rates in the mixed layer to within a factor of two.
Sector‐Based Top‐Down Estimates of NOx, SO2, and CO Emissions in East Asia
Authors: Qu Zhen , Henze Daven K. , Worden Helen M. , Jiang Zhe , Gaubert Benjamin , Theys Nicolas , Wang Wei
Published: January 2022

Top‐down estimates using satellite data provide important information on the sources of air pollutants. We develop a sector‐based 4D‐Var framework based on the GEOS‐Chem adjoint model to address the impacts of co‐emissions and chemical interactions on top‐down emission estimates. We apply OMI NO2, OMI SO2, and MOPITT CO observations to estimate NOx, SO2, and CO emissions in East Asia during 2005–2012. Posterior evaluations with surface measurements show reduced normalized mean bias (NMB) by 7% (NO2)–15% (SO2) and normalized mean square error (NMSE) by 8% (SO2)–9% (NO2) compared to a species‐based inversion. This new inversion captures the peak years of Chinese SO2 (2007) and NOx (2011) emissions and attributes their drivers to industry and energy activities. The CO peak in 2007 in China is driven by residential and industry emissions. In India, the inversion attributes NOx and SO2 trends mostly to energy and CO trend to residential emissions.
Improved Simulation of ENSO Variability Through Feedback From the Equatorial Atlantic in a Pacemaker Experiment
Authors: Bi Daohua , Wang Guojian , Cai Wenju , Santoso Agus , Sullivan Arnold , Ng Benjamin , Jia Fan
Published: January 2022

The tropical Pacific Ocean interacts with the tropical Atlantic Ocean on interannual time scales. However, most coupled climate models misrepresent and/or underestimate these teleconnections, which have important implications on the periodicity of the El Niño‐Southern Oscillation (ENSO). Imposing the observed sea surface temperature over the tropical Atlantic from 1970 onwards, a pacemaker experiment is constructed using the second version of the Australian Community Climate and Earth System Simulator Coupled Model (ACCESS‐CM2). Compared with control runs which reasonably simulate the interaction between ENSO and the north tropical Atlantic variability, the pacemaker experiment significantly improves the impact of the Atlantic Niño/Niña on ENSO. This enhanced teleconnection contributes to a more realistic ENSO periodicity compared with the control runs, thus suggesting that capturing the influence from equatorial Atlantic variability is important for an improved ENSO simulation.
Transitions of Dissolution Patterns in Rough Fractures
Authors: Wang Ting , Hu Ran , Yang Zhibing , Zhou Chen‐Xing , Chen Yi‐Feng , Zhou Chuang‐Bing
Published: January 2022

Rock fractures are ubiquitous in geological systems and usually provide dominant pathways for fluid flow in fractured reservoirs. When the flowing fluid is reactive, fracture dissolution expands the aperture and forms various dissolution patterns that amplify the pathways. Previous works focused on the dissolution processes in Hele‐Shaw cells (parallel‐plate) and porous media, but the transitions of dissolution patterns in radial rough fractures are not well understood. Here we combine flow‐visualization experiments with theoretical analysis to elucidate the transitions of dissolution patterns under various flow‐rate and reaction‐rate conditions. We observe and quantify three distinct dissolution morphologies as compact, wormhole, and uniform patterns. We show that the critical Péclet numbers, corresponding to the transitions from compact to wormhole and to uniform patterns, increase with the reaction rate. Based on the growth of dissolution channels in the ﬂow and transverse directions, we establish a theoretical model that describes the transitions of these three distinct dissolution patterns. The phase diagram predicted by the model exhibits good agreement with our experimental results and also well captures the pattern transitions reported in the previous studies. This work improves the understanding on how fracture aperture expands in the dissolution processes that lead to various dissolution channels. Our work is also critical for controlling the fluid flow behavior in dissolving natural rock fractures in subsurface flow systems.
Insights into the multifaceted role of circular RNAs: implications for Parkinson’s disease pathogenesis and diagnosis
Authors: Doxakis Epaminondas
Published: January 2022

Parkinson’s disease (PD) is a complex, age-related, neurodegenerative disease whose etiology, pathology, and clinical manifestations remain incompletely understood. As a result, care focuses primarily on symptoms relief. Circular RNAs (circRNAs) are a large class of mostly noncoding RNAs that accumulate with aging in the brain and are increasingly shown to regulate all aspects of neuronal and glial development and function. They are generated by the spliceosome through the backsplicing of linear RNA. Although their biological role remains largely unknown, they have been shown to regulate transcription and splicing, act as decoys for microRNAs and RNA binding proteins, used as templates for translation, and serve as scaffolding platforms for signaling components. Considering that they are stable, diverse, and detectable in easily accessible biofluids, they are deemed promising biomarkers for diagnosing diseases. CircRNAs are differentially expressed in the brain of patients with PD, and growing evidence suggests that they regulate PD pathogenetic processes. Here, the biogenesis, expression, degradation, and detection of circRNAs, as well as their proposed functions, are reviewed. Thereafter, research linking circRNAs to PD-related processes, including aging, alpha-synuclein dysregulation, neuroinflammation, and oxidative stress is highlighted, followed by recent evidence for their use as prognostic and diagnostic biomarkers for PD.
Cuffless blood pressure monitoring from a wristband with calibration-free algorithms for sensing location based on bio-impedance sensor array and autoencoder
Authors: Ibrahim Bassem , Jafari Roozbeh
Published: January 2022

Continuous monitoring of blood pressure (BP) is essential for the prediction and the prevention of cardiovascular diseases. Cuffless BP methods based on non-invasive sensors integrated into wearable devices can translate blood pulsatile activity into continuous BP data. However, local blood pulsatile sensors from wearable devices suffer from inaccurate pulsatile activity measurement based on superficial capillaries, large form-factor devices and BP variation with sensor location which degrade the accuracy of BP estimation and the device wearability. This study presents a cuffless BP monitoring method based on a novel bio-impedance (Bio-Z) sensor array built in a flexible wristband with small-form factor that provides a robust blood pulsatile sensing and BP estimation without calibration methods for the sensing location. We use a convolutional neural network (CNN) autoencoder that reconstructs an accurate estimate of the arterial pulse signal independent of sensing location from a group of six Bio-Z sensors within the sensor array. We rely on an Adaptive Boosting regression model which maps the features of the estimated arterial pulse signal to systolic and diastolic BP readings. BP was accurately estimated with average error and correlation coefficient of 0.5 ± 5.0 mmHg and 0.80 for diastolic BP, and 0.2 ± 6.5 mmHg and 0.79 for systolic BP, respectively.
Structural characterization of human importin alpha 7 in its cargo-free form at 2.5 Å resolution
Authors: Tsimbalyuk S. , Donnelly C. M. , Forwood J. K.
Published: January 2022

Shuttling of macromolecules between nucleus and cytoplasm is a tightly regulated process mediated through specific interactions between cargo and nuclear transport proteins. In the classical nuclear import pathway, importin alpha recognizes cargo exhibiting a nuclear localization signal, and this complex is transported through the nuclear pore complex by importin beta. Humans possess seven importin alpha isoforms that can be grouped into three subfamilies, with many cargoes displaying specificity towards these importin alpha isoforms. The cargo binding sites within importin alpha isoforms are highly conserved in sequence, suggesting that specificity potentially relies on structural differences. Structures of some importin alpha isoforms, both in cargo-bound and free states, have been previously solved. However, there are currently no known structures of cargo free importin alpha isoforms within subfamily 3 (importin alpha 5, 6, 7). Here, we present the first crystal structure of human importin alpha 7 lacking the IBB domain solved at 2.5 Å resolution. The structure reveals a typical importin alpha architecture comprised of ten armadillo repeats and is most structurally conserved with importin alpha 5. Very little difference in structure was observed between the cargo-bound and free states, implying that importin alpha 7 does not undergo conformational change when binding cargo. These structural insights provide a strong platform for further evaluation of structure–function relationships and understanding how isoform specificity within the importin alpha family plays a role in nuclear transport in health and disease.
Microwave versus radiofrequency ablation for the treatment of liver malignancies: a randomized controlled phase 2 trial
Authors: Radosevic Aleksandar , Quesada Rita , Serlavos Clara , Sánchez Juan , Zugazaga Ander , Sierra Ana , Coll Susana , Busto Marcos , Aguilar Guadalupe , Flores Daniel , Arce Javier , Maiques José María , Garcia-Retortillo Montserrat , Carrion José Antonio , Visa Laura , Villamonte María , Pueyo Eva , Berjano Enrique , Trujillo Macarena , Sánchez-Velázquez Patricia , Grande Luís , Burdio Fernando
Published: January 2022

Microwave (MWA) and radiofrequency ablation (RFA) are main ablative techniques for hepatocellular carcinoma (HCC) and colorectal liver metastasis (MT). This randomized phase 2 clinical trial compares the effectiveness of MWA and RFA as well as morphology of corresponding ablation zones. HCC and MT patients with 1.5–4 cm tumors, suitable for ablation, were randomized into MWA or RFA Groups. The primary endpoint was short-to-long diameter ratio of ablation zone (SLR). Primary technical success (TS) and a cumulative local tumor progression (LTP) after a median 2-year follow-up were compared. Between June 2015 and April 2020, 82 patients were randomly assigned (41 patients per group). For the per-protocol analysis, five patients were excluded. MWA created larger ablation zones than RFA (p = 0.036) although without differences in SLR (0.5 for both groups, p = 0.229). The TS was achieved in 98% (46/47) and 90% (45/50) (p = 0.108), and LTP was observed in 21% (10/47) vs. 12% (6/50) (OR 1.9 [95% CI 0.66–5.3], p = 0.238) of tumors in MWA vs. RFA Group, respectively. Major complications were found in 5 cases (11%) vs. 2 cases (4%), without statistical significance. MWA and RFA show similar SLR, effectiveness and safety in liver tumors between 1.5 and 4 cm.
Assessing the potential of a Trichoderma-based compost activator to hasten the decomposition of incorporated rice straw
Authors: Organo Nolissa D. , Granada Shaira Mhel Joy M. , Pineda Honey Grace S. , Sandro Joseph M. , Nguyen Van Hung , Gummert Martin
Published: January 2022

The potential for a Trichoderma-based compost activator was tested for in-situ rice straw decomposition, under both laboratory and field conditions. Inoculation of Trichoderma caused a 50% reduction in the indigenous fungal population after 2 weeks of incubation for both laboratory and field experiments. However, the Trichoderma population declined during the latter part of the incubation. Despite the significant reduction in fungal population during the first 2 weeks of incubation, inoculated samples were found to have higher indigenous and total fungal population at the end of the experiments with as much as a 300% increase in the laboratory experiment and 50% during day-21 and day-28 samplings in the field experiment. The laboratory incubation experiment revealed that inoculated samples released an average of 16% higher amounts of CO2 compared to uninoculated straw in sterile soil samples. Unsterile soil inoculated with Trichoderma released the highest amount of CO2 in the laboratory experiment. In the field experiment, improved decomposition was observed in samples inoculated with Trichoderma and placed below ground (WTBG). From the initial value of around 35%, the C content in WTBG was down to 28.63% after 42 days of incubation and was the lowest among treatments. This is significantly lower compared with NTBG (No Trichoderma placed below ground, 31.1% C), WTSS (With Trichoderma placed on soil surface, 33.83% C), and NTSS (No Trichoderma placed on soil surface, 34.30% carbon). The WTBG treatment also had the highest N content of 1.1%. The C:N ratio of WTBG was only 26.27, 39.51% lower than the C:N ratio of NTBG, which is 43.43. These results prove that the Trichoderma-based inoculant has the potential to hasten the decomposition of incorporated rice straw.
Efficacy and safety of 3D printing-assisted percutaneous nephrolithotomy in complex renal calculi
Authors: Cui Dong , Yan Fengqi , Yi JiangPu , He Dali , Zhang Yichen , Zhang Zekai , Chen Yuntao , Jiao Yong , Zhang Bo
Published: January 2022

This study evaluated the efficacy and safety of 3D printing technology combined with percutaneous nephrolithotomy in the treatment of complex renal calculi. Ninety patients with complex renal calculi were randomly divided into a 3D printing group (45 patients) and a control group (45 patients). In the 3D printing group, a patient-specific 1:1 3D printing model was established based on the patient's thin-layer CT scanning data. A 3D printing model was used for preoperative communication between doctors and patients. Preoperative puncture training, channel design, residual stone prediction, and percutaneous nephrolithotomy were performed under the guidance of a 3D printing model and B-ultrasound. The control group was treated with the conventional B-ultrasound-guided puncture method. Results suggest that there was a statistically significant difference between the two groups (P < 0.05). The overall score of the doctor-patient communication objects in the 3D printing group was 19.32 ± 1.57 points, and in the control group, it was 14.51 ± 2.13 points. The operation time of the 3D printing group was 103.21 ± 13.49 min, and that of the control group was 126.12 ± 25.87 min. The calculi clearance rate of the 3D printing group was 96%, while that of the control group was 80%. The incidence of postoperative complications was 6.67% in the 3D printing group and 22.22% in the control group. Compared with traditional percutaneous nephrolithotomy, 3D printing technology combined with percutaneous nephrolithotomy can significantly enhance the effectiveness of doctor–patient communication, shorten operation time, reduce operation bleeding, improve the stone clearance rate, reduce the incidence of complications and shorten the length of hospital stay. The proposed method is thus a safe and effective method to treat complex renal calculi.
Brachial artery transposition versus catheters as tertiary vascular access for maintenance hemodialysis: a single-center retrospective study
Authors: Soma Yu , Murakami Masaaki , Nakatani Eiji , Sato Yoko , Tanaka Satoshi , Mori Kiyoshi , Sugawara Akira
Published: January 2022

Some hemodialysis patients are not suitable for creation of an arteriovenous fistula (AVF) or arteriovenous graft (AVG). However, they can receive a tunneled cuffed central venous catheter (tcCVC), but this carries risks of infection and mortality. We aimed to evaluate the safety and effectiveness of brachial artery transposition (BAT) versus those of tcCVC. This retrospective study evaluated hemodialysis patients who underwent BAT or tcCVC placement because of severe heart failure, hand ischemia, central venous stenosis or occlusion, inadequate vessels for creating standard arteriovenous access, or limited life expectancy. The primary outcome was whole access circuit patency. Thirty-eight patients who underwent BAT and 25 who underwent tcCVC placement were included. One-year patency rates for the whole access circuit were 84.6% and 44.9% in the BAT and tcCVC groups, respectively. The BAT group was more likely to maintain patency (unadjusted hazard ratio: 0.17, 95% confidence interval: 0.05–0.60, p = 0.006). The two groups did not have significantly different overall survival (log-rank p = 0.146), although severe complications were less common in the BAT group (3% vs. 28%, p = 0.005). Relative to tcCVC placement, BAT is safe and effective with acceptable patency in hemodialysis patients not suitable for AVF or AVG creation.
Simple questionnaires outperform behavioral tasks to measure socio-emotional skills in students
Authors: Boon-Falleur Mélusine , Bouguen Adrien , Charpentier Axelle , Algan Yann , Huillery Élise , Chevallier Coralie
Published: January 2022

Recent empirical research has shown that improving socio-emotional skills such as grit, conscientiousness and self-control leads to higher academic achievement and better life outcomes. However, both theoretical and empirical works have raised concerns about the reliability of the different methods used to measure socio-emotional skills. We compared the reliability and validity of the three leading measurements methods—a student-reported questionnaire, a teacher-reported questionnaire, and a behavioral task—in a sample of 3997 French students. Before analyzing the data, we polled 114 international researchers in cognitive development and education economics; most researchers in both fields predicted that the behavioral task would be the best method. We found instead that the teacher questionnaire was more predictive of students’ behavioral outcomes and of their grade progression, while the behavioral task was the least predictive. This work suggests that researchers may not be using optimal tools to measure socio-emotional skills in children.
Breath biomarkers of insulin resistance in pre-diabetic Hispanic adolescents with obesity
Authors: Khan Mohammad S. , Cuda Suzanne , Karere Genesio M. , Cox Laura A. , Bishop Andrew C.
Published: January 2022

Insulin resistance (IR) affects a quarter of the world’s adult population and is a major factor in the pathogenesis of cardio-metabolic disease. In this pilot study, we implemented a non-invasive breathomics approach, combined with random forest machine learning, to investigate metabolic markers from obese pre-diabetic Hispanic adolescents as indicators of abnormal metabolic regulation. Using the ReCIVA breathalyzer device for breath collection, we have identified a signature of 10 breath metabolites (breath-IR model), which correlates with Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) (R = 0.95,p < 0.001). A strong correlation was also observed between the breath-IR model and the blood glycemic profile (fasting insulin R = 0.91,p < 0.001 and fasting glucose R = 0.80,p < 0.001). Among tentatively identified metabolites, limonene, undecane, and 2,7-dimethyl-undecane, significantly cluster individuals based on HOMA-IR (p = 0.003,p = 0.002, andp < 0.001, respectively). Our breath-IR model differentiates between adolescents with and without IR with an AUC-ROC curve of 0.87, after cross-validation. Identification of a breath signature indicative of IR shows utility of exhaled breath metabolomics for assessing systemic metabolic dysregulation. A simple and non-invasive breath-based test has potential as a diagnostic tool for monitoring IR progression, allowing for earlier detection of IR and implementation of early interventions to prevent onset of type 2 diabetes mellitus.
RNAseq reveals extensive metabolic disruptions in the sensitive SF-295 cell line treated with schweinfurthins
Authors: Weissenrieder J. S. , Weissenkampen J. D. , Reed J. L. , Green M. V. , Zheng C. , Neighbors J. D. , Liu D. J. , Hohl Raymond J.
Published: January 2022

The schweinfurthin family of natural compounds exhibit a unique and potent differential cytotoxicity against a number of cancer cell lines and may reduce tumor growth in vivo. In some cell lines, such as SF-295 glioma cells, schweinfurthins elicit cytotoxicity at nanomolar concentrations. However, other cell lines, like A549 lung cancer cells, are resistant to schweinfurthin treatment up to micromolar concentrations. At this time, the precise mechanism of action and target for these compounds is unknown. Here, we employ RNA sequencing of cells treated with 50 nM schweinfurthin analog TTI-3066 for 6 and 24 h to elucidate potential mechanisms and pathways which may contribute to schweinfurthin sensitivity and resistance. The data was analyzed via an interaction model to observe differential behaviors between sensitive SF-295 and resistant A549 cell lines. We show that metabolic and stress-response pathways were differentially regulated in the sensitive SF-295 cell line as compared with the resistant A549 cell line. In contrast, A549 cell had significant alterations in response genes involved in translation and protein metabolism. Overall, there was a significant interaction effect for translational proteins, RNA metabolism, protein metabolism, and metabolic genes. Members of the Hedgehog pathway were differentially regulated in the resistant A549 cell line at both early and late time points, suggesting a potential mechanism of resistance. Indeed, when cotreated with the Smoothened inhibitor cyclopamine, A549 cells became more sensitive to schweinfurthin treatment. This study therefore identifies a key interplay with the Hedgehog pathway that modulates sensitivity to the schweinfurthin class of compounds.
A randomised controlled trial evaluating the effect of a brief motivational intervention to promote breastfeeding in postpartum depression
Authors: Franco-Antonio C. , Santano-Mogena E. , Chimento-Díaz S. , Sánchez-García P. , Cordovilla-Guardia S.
Published: January 2022

Postpartum depression (PPD) is the most frequent psychiatric complication during the postnatal period. According to existing evidence, an association exists between the development of PPD and the maintenance of breastfeeding. A brief motivational intervention (bMI), based on the motivational interview, seems effective in promoting breastfeeding. The objective of this study was to analyse the impact of a bMI aiming to promote breastfeeding on the development of PPD and explore the mediating/moderating roles of breastfeeding and breastfeeding self-efficacy in the effect of the intervention on developing PPD. Eighty-eight women who gave birth by vaginal delivery and started breastfeeding during the immediate postpartum period were randomly assigned to the intervention group (bMI) or control group (breastfeeding education). Randomisation by minimisation was carried out. The breastfeeding duration was longer in the intervention group (11.06 (± 2.94) weeks vs 9.02 (± 4.44), p = 0.013). The bMI was associated with a lower score on the Edinburgh Postnatal Depression Scale, with a regression β coefficient of − 2.12 (95% CI − 3.82; − 0.41). A part of this effect was mediated by the effect of the intervention on the duration of breastfeeding (mediation/moderation index β = − 0.57 (95% CI − 1.30; − 0.04)). These findings suggest that a bMI aiming to promote breastfeeding has a positive impact preventing PPD mainly due to its effectiveness in increasing the duration of breastfeeding.
Characterization of cisplatin-loaded chitosan nanoparticles and rituximab-linked surfaces as target-specific injectable nano-formulations for combating cancer
Authors: Sultan Muhammad H. , Moni Sivakumar S. , Madkhali Osama A. , Bakkari Mohammed Ali , Alshahrani Saeed , Alqahtani Saad S. , Alhakamy Nabil A. , Mohan Syam , Ghazwani Mohammed , Bukhary Haitham A. , Almoshari Yosif , Salawi Ahmad , Alshamrani Meshal
Published: January 2022

The present study was carried out to develop cisplatin-loaded chitosan nanoparticles (CCNP) and cisplatin-loaded chitosan nanoparticle surface linked to rituximab (mAbCCNP) as targeted delivery formulations. The two formulations (CCNP and mAbCCNP) exhibited significant physicochemical properties. The zetapotential (ZP) values of CCNP and mAbCCNP were 30.50 ± 5.64 and 26.90 ± 9.09 mV, respectively; while their particle sizes were 308.10 ± 1.10 and 349.40 ± 3.20 z.d.nm, respectively. The poly dispersity index (PDI) of CCNP was 0.257 ± 0.030 (66.6% PDI), while that of mAbCCNP was 0.444 ± 0.007 (57.60% PDI). Differential scanning calorimetry (DSC) revealed that CCNP had endothermic peaks at temperatures ranging from 135.50 to 157.69 °C. A sharp exothermic peak was observed at 95.79 °C, and an endothermic peak was observed at 166.60 °C. The XRD study on CCNP and mAbCCNP revealed distinct peaks at 2θ. Four peaks at 35.38°, 37.47°, 49.29°, and 59.94° corresponded to CCNP, while three distinct peaks at 36.6°, 49.12°, and 55.08° corresponded to mAbCCNP. The in vitro release of cisplatin from nanoparticles followed zero order kinetics in both CCNP and mAbCCNP. The profile for CCNP showed 43.80% release of cisplatin in 6 h (R2 = 0.9322), indicating linearity of release with minimal deviation. However, the release profile of mAbCCNP showed 22.52% release in 4 h (R2 = 0.9416), indicating linearity with sustained release. In vitro cytotoxicity studies on MCF-7 ATCC human breast cancer cell line showed that CCNP exerted good cytotoxicity, with IC50 of 4.085 ± 0.065 µg/mL. However, mAbCCNP did not elicit any cytotoxic effect. At a dose of 4.00 µg/mL cisplatin induced early apoptosis and late apoptosis, chromatin condensation, while it produced secondary necrosis at a dose of 8.00 µg/mL. Potential delivery system for cisplatin CCNP and mAbCCNP were successfully formulated. The results indicated that CCNP was a more successful formulation than mAbCCNP due to lack of specificity of rituximab against MCF-7 ATCC human breast cancer cells.
Coordination dynamics of multi-agent interaction in a musical ensemble
Authors: Proksch Shannon , Reeves Majerle , Spivey Michael , Balasubramaniam Ramesh
Published: January 2022

Humans interact with other humans at a variety of timescales and in a variety of social contexts. We exhibit patterns of coordination that may differ depending on whether we are genuinely interacting as part of a coordinated group of individuals vs merely co-existing within the same physical space. Moreover, the local coordination dynamics of an interacting pair of individuals in an otherwise non-interacting group may spread, propagating change in the global coordination dynamics and interaction of an entire crowd. Dynamical systems analyses, such as Recurrence Quantification Analysis (RQA), can shed light on some of the underlying coordination dynamics of multi-agent human interaction. We used RQA to examine the coordination dynamics of a performance of “Welcome to the Imagination World”, composed for wind orchestra. This performance enacts a real-life simulation of the transition from uncoordinated, non-interacting individuals to a coordinated, interacting multi-agent group. Unlike previous studies of social interaction in musical performance which rely on different aspects of video and/or acoustic data recorded from each individual, this project analyzes group-level coordination patterns solely from the group-level acoustic data of an audio recording of the performance. Recurrence and stability measures extracted from the audio recording increased when musicians coordinated as an interacting group. Variability in these measures also increased, indicating that the interacting ensemble of musicians were able to explore a greater variety of behavior than when they performed as non-interacting individuals. As an orchestrated (non-emergent) example of coordination, we believe these analyses provide an indication of approximate expected distributions for recurrence patterns that may be measurable before and after truly emergent coordination.
PRMT3 inhibitor SGC707 reduces triglyceride levels and induces pruritus in Western-type diet-fed LDL receptor knockout mice
Authors: de Jong Laura M. , Zhang Zhengzheng , den Hartog Yvette , Sijsenaar Timothy J. P. , Martins Cardoso Renata , Manson Martijn L. , Hankemeier Thomas , Lindenburg Peter W. , Salvatori Daniela C. F. , Van Eck Miranda , Hoekstra Menno
Published: January 2022

Protein arginine methyltransferase 3 (PRMT3) is a co-activator of liver X receptor capable of selectively modulating hepatic triglyceride synthesis. Here we investigated whether pharmacological PRMT3 inhibition can diminish the hepatic steatosis extent and lower plasma lipid levels and atherosclerosis susceptibility. Hereto, male hyperlipidemic low-density lipoprotein receptor knockout mice were fed an atherogenic Western-type diet and injected 3 times per week intraperitoneally with PRMT3 inhibitor SGC707 or solvent control. Three weeks into the study, SGC707-treated mice developed severe pruritus and scratching-associated skin lesions, leading to early study termination. SGC707-treated mice exhibited 50% lower liver triglyceride stores as well as 32% lower plasma triglyceride levels. Atherosclerotic lesions were virtually absent in all experimental mice. Plasma metabolite analysis revealed that levels of taurine-conjugated bile acids were ~ threefold increased (P < 0.001) in response to SGC707 treatment, which was paralleled by systemically higher bile acid receptor TGR5 signalling. In conclusion, we have shown that SGC707 treatment reduces hepatic steatosis and plasma triglyceride levels and induces pruritus in Western-type diet-fed LDL receptor knockout mice. These findings suggest that pharmacological PRMT3 inhibition can serve as therapeutic approach to treat non-alcoholic fatty liver disease and dyslipidemia/atherosclerosis, when unwanted effects on cholesterol and bile acid metabolism can be effectively tackled.
A 2-month field cohort study of SARS-CoV-2 in saliva of BNT162b2 vaccinated nursing home workers
Authors: Saegerman Claude , Diep Anh Nguyet , Renault Véronique , Donneau Anne-Françoise , Stamatakis Lambert , Coppieters Wouter , Michel Fabienne , Breuer Christophe , Dandoy Margaux , Ek Olivier , Gourzones Claire , Schyns Joey , Goffin Emeline , Minner Frédéric , Durkin Keith , Artesi Maria , Bours Vincent , Bureau Fabrice , Gillet Laurent
Published: January 2022

Background

Nursing home (NH) residents have been severely affected during the COVID-19 pandemic because of their age and underlying comorbidities. Infection and outbreaks in NHs are most likely triggered by infected workers. Screening for asymptomatic NH workers can prevent risky contact and viral transmission to the residents. This study examined the effect of the BNT162b2 mRNA COVID‑19 (Comirnaty®; BioNTech and Pfizer) vaccination on the saliva excretion of SARS-CoV-2 among NH workers, through weekly saliva RT-qPCR testing.

Methods

A 2-month cohort study was conducted among 99 NHs in the Walloon region (Belgium), at the start of February 2021. Three groups of workers, i.e., non-vaccinated (n = 1618), one-dosed vaccinated (n = 1454), and two-dosed vaccinated (n = 2379) of BNT162b2 mRNA COVID‑19 vaccine, were followed-up weekly. Their saliva samples were used to monitor the shedding of SARS-CoV-2. All positive samples were sequenced and genotyped to identify the circulating wild-type virus or variants of concern.

Results

The protection fraction against the excretion of the SARS-CoV-2 in the saliva samples of the workers after the second dose is estimated at 0.90 (95% CI: 0.18; 0.99) at 1 week and 0.83 (95% CI: 0.54; 0.95) at 8 weeks. We observe more circulating SARS-CoV-2 and a greater variability of viral loads in the unvaccinated group compared to those of the vaccinated group.

Conclusions

This field cohort study advances our knowledge of the efficacy of the mRNA BNT162b2 COVID-19 vaccine on the viral shedding in the saliva specimens of vaccinated NH workers, contributing to better decision-making in public health interventions and management.
Synthesis of β-nitro ketones from geminal bromonitroalkanes and silyl enol ethers by visible light photoredox catalysis
Authors: Cao Haoying , Ma Shanshan , Feng Yanhong , Guo Yawen , Jiao Peng
Published: January 2022

Photocatalytic coupling reaction between a TMS enol ether and a gem-bromonitroalkane furnishes a ketone bearing β-NO2 group on a tertiary carbon, which is easily converted into a β-NH2 ketone. α-Nitro radicals are intermediates.
A switchable terahertz device combining ultra-wideband absorption and ultra-wideband complete reflection
Authors: Zheng Zhipeng , Zheng Ying , Luo Yao , Yi Zao , Zhang Jianguo , Liu Zhimin , Yang Wenxing , Yu Yang , Wu Xianwen , Wu Pinghui
Published: January 2022

A metamaterial absorber based on the thermotropic phase change material VO2 has been designed. Flexible switching of absorption performance (bandwidth is 3.3 THz) can be achieved through temperature adjustment.
Double hydrophilic copolymers – synthetic approaches, architectural variety, and current application fields
Authors: Nabiyan Afshin , Max Johannes B. , Schacher Felix H.
Published: January 2022

Double hydrophilic copolymers consist of two chemically different but water-soluble segments. We present synthetic approaches and both established as well as emerging applications such as photocatalysis or sensing.
Robust MOF-derived carbon-supported bimetallic Ni–Co catalysts for aqueous phase hydrodeoxygenation of vanillin
Authors: Zhang Yunpeng , Zhao Jingwen , Fan Guoli , Yang Lan , Li Feng
Published: January 2022

MOF-derived carbon-supported bimetallic Ni–Co catalysts exhibited excellent catalytic activity and high stability for aqueous phase hydrodeoxygenation of vanillin.
Synthesis of rhombohedral LaCuO3 thin films using the oxidation effect of NaClO solution
Authors: Asanuma Shutaro
Published: January 2022

In this study, I demonstrated rhombohedral LaCuO3 thin film can be synthesized without using the high-pressure and high-temperature method but using the oxidation effect of NaClO solution.
Sustainability-inspired upcycling of waste polyethylene terephthalate plastic into porous carbon for CO2 capture
Authors: Yuan Xiangzhou , Kumar Nallapaneni Manoj , Brigljević Boris , Li Shuangjun , Deng Shuai , Byun Manhee , Lee Boreum , Lin Carol Sze Ki , Tsang Daniel C. W. , Lee Ki Bong , Chopra Shauhrat S. , Lim Hankwon , Ok Yong Sik
Published: January 2022

Industrial-scale upcycling of waste polyethylene terephthalate (PET) plastic into porous carbon globally for CO2 capture was verified as a multifunctional alternative to conventional CO2 absorption and plastic waste management technologies.
A floating 5 μm-diameter needle electrode on the tissue for damage-reduced chronic neuronal recording in mice
Authors: Yamashita Koji , Sawahata Hirohito , Yamagiwa Shota , Yokoyama Shohei , Numano Rika , Koida Kowa , Kawano Takeshi
Published: January 2022

We propose neural recording using a 5 μm-diameter needle electrode device, which enables following the brain's motion. The implanted device in the mouse brain showed stable recording of neuronal signals for 6 months and reduced tissue damage.
Composite chitosan and quaternary ammonium modified nanofibrillar cellulose anion exchange membranes for direct ethanol fuel cell applications
Authors: Hren Maša , Gorgieva Selestina
Published: January 2022

Fuel cells are a promising technology for energy production, but their commercialization is hindered mainly due to high costs. Direct alkaline ethanol fuel cells (DAFC) are receiving increasing attention as they can utilize cheaper, non-precious metal catalysts. A vital component of a DAFC is the anion exchange membrane (AEM). Currently, the commercially available AEMs don’t possess satisfactory properties. This indicates a need for the development of new highly efficient, environmentally friendly, and economically viable AEMs. Synthesis of synthetic polymer AEMs is usually complex and time-consuming, as well as environmentally unfriendly. Therefore, it is highly desired that the membrane material is bio-renewable, non-toxic and environmentally benign. In this work, a series of biopolymer membranes were designed by a simple, cost-effective, dispersion-casting procedure, fully complying with green-chemistry principles. Design of experiments was used as a methodology for identifying optimal combinations of influencing factors and their relations within selected responses. The obtained chitosan-Mg(OH)2 composite membranes containing modified nanofibrillar cellulose (CNF) fillers with quaternary ammonium groups were investigated for their mechanical properties, swelling ratio, ethanol permeability and ion exchange properties. Obtained data suggest the applicability of newly prepared, biopolymeric composites as eco-friendly AEMs in DAFC technologies.
Characterization and experimental comparison of commercial PEMFC stacks for marine applications
Authors: Gadducci Eleonora , Lamberti Thomas , Magistri Loredana , Rivarolo Massimo , Dellacasa Andrea , Campora Barbara , Borgogna Gerardo , Lancella Agnese , Speranza Enrico , Voiello Andrea
Published: January 2022

PEM Fuel Cells are considered among the most promising technologies for hydrogen utilization in both stationary and automotive applications. The number of FC installations on board ships – alone or in hybrid configuration with batteries – is increasing significantly, although international regulations that drive their installation are still missing. In this scenario, the project TecBia aims to identify a dedicated test protocol and the best commercial PEMFC technology for marine applications, assessing the integration of a 140 kW PEMFC system on the Zero Emission Ultimate Ship (ZEUS) vessel. The system design and technology provider has been chosen after a technical comparison based on a dedicated experimental campaign. The experimental campaign had two goals: (i) analyse the performance of the different PEMFC systems to define the best characteristics for maritime applications; (ii) verify the compliance with naval requirements with reference to current and future standards. The present study shows the resulting test protocol for FC Systems (FCS) for maritime applications, defined starting from the existing international regulations on FCS installations and on naval environment requirements; the results of its application on the commercial system chosen for the installation on ZEUS are reported.
Single step polyol synthesis of highly stable Pt/C/SiO2 catalysts for use in PEMFCs: effects of pH, temperature and W/EG ratio
Authors: Sousa Eva , Delgado Sofia , Lagarteira Tiago , Mendes Adélio
Published: January 2022

Hybrid supports have been proposed as a new alternative to increase the stability of ORR catalysts used in PEMFCs for automotive applications since they are known to be stable under harsh conditions. In this work, Pt nanoparticles were deposited over C/SiO2, via single-step polyol method, to take advantage of the corrosion-resistance properties of silica nanoparticles. In fact, the synthesis parameters, namely, pH, temperature, and glycol concentration had a remarkable impact on the Pt size-distribution, crystallinity, and dispersion over the C/SiO2 supports. A maximum ORR activity and stability was obtained for Pt/C/SiO2 catalysts produced at 1:6 W/EG (v/v). The addition of SiO2 nanoparticles to the carbon structure showed their ability to effectively inhibit support corrosion and Pt nanoparticles detachment and/or growth, with the pH adjustments being critical for obtaining highly stable C/SiO2 supports. Pt/C/SiO2 synthetized under acidic conditions revealed the highest stability when subjected to accelerated stress tests (ASTs), losing only 30 % of the initial electrochemically active surface area (ECSA) of Pt after 4 000 cycles from 0.6 to 1 V (vs RHE), whereas the commercial Pt/C revealed > 50 % of ECSA loss.
Response Surface Methodology for 30 kW PEMFC stack characterization
Authors: Gadducci Eleonora , Saccaro Stefano , Rivarolo Massimo , Magistri Loredana
Published: January 2022

Hydrogen is a promising energy carrier to allow the reach of the zero-emission targets established for the next years. Polymeric Electrolyte Membrane FC are studied inside the HI-SEA laboratory of the University of Genoa, to assess the opportunities of this technology on marine applications. Here, 8 PEMFC stacks, sized 30 kW each for a total power installation of 240 kW, have been tested to draw guidelines for the best system design onboard ships and to deepen the know-how on the experimental management of the technology. During the tests, it was possible to observe the reciprocal influence of some parameters, which may influence the system efficiency. In this work, a statistical investigation is developed to quantify the cell voltage variation correlated to the values of temperature and current. This has been possible thanks to Design Expert (DE), a software developed by Stat-EASE, Inc. Through the Design of Experiment approach, it is possible to evaluate the significance of variables in the FC system, called factors. The experiment under consideration is also characterized by non-controllable factors, cause of disturbances that induce further variability in the response. Eventually, it was possible to analyse the significance of the parameters involved, to build a regression model by performing the analysis of variance with which the significant values are identified, and to assess the presence of outliers.