bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemical, Nutritional and Biological Evaluation of a Sustainable and Scalable Complex of Phytochemicals from Bergamot By-Products
Authors: Della Vedova Larissa , Gado Francesca , Vieira Taynara A. , Grandini Núbia A. , Palácio Thiago L. N. , Siqueira Juliana S. , Carini Marina , Bombardelli Ezio , Correa Camila R. , Aldini Giancarlo , Baron Giovanna
Published: March 2023

The present paper reports a sustainable raw material obtained from the by-products derived from the industrial production of bergamot (Citrus × Bergamia Risso & Poiteau) essential oils. The procedure to obtain the raw material is designed to maintain as much of the bioactive components as possible and to avoid expensive chemical purification. It consists of spray-drying the fruit juice obtained by squeezing the fruits, which is mixed with the aqueous extract of the pulp, i.e., the solid residue remained after fruit pressing. The resulting powder bergamot juice (PBJ) contains multiple bioactive components, in particular, among others, soluble fibers, polyphenols and amino-acid betaines, such as stachydrine and betonicine. LC-MS analysis identified 86 compounds, with hesperetin, naringenin, apigenin and eridictyol glucosides being the main components. In the second part of the paper, dose-dependent anti-inflammatory activity of PBJ and of stachydrine was found, but neither of the compounds were effective in activating Nrf2. PBJ was then found to be effective in an in vivo model of a metabolic syndrome induced by a high-sugar, high-fat (HSF) diet and evidenced by a significant increase of the values related to a set of parameters: blood glucose, triglycerides, insulin resistance, systolic blood pressure, visceral adipose tissue and adiposity index. PBJ, when given to control rats, did not significantly change these values; in contrast, they were found to be greatly affected in rats receiving an HSF diet. The in vivo effect of PBJ can be ascribed not only to bergamot polyphenols with well-known anti-inflammatory, antioxidant and lipid-regulating effects, but also to the dietary fibers and to the non-phenolic constituents, such as stachydrine. Moreover, since PBJ was found to affect energy homeostasis and to regulate food intake, a mechanism on the regulation of energy homeostasis through leptin networking should also be considered and deserves further investigation.
EAE of Mice: Enzymatic Cross Site-Specific Hydrolysis of H2B Histone by IgGs against H1, H2A, H2B, H3, and H4 Histones and Myelin Basic Protein
Authors: Urusov Andrey E. , Aulova Kseniya S. , Dmitrenok Pavel S. , Buneva Valentina N. , Nevinsky Georgy A.
Published: March 2023

Histones have vital roles in chromatin functioning and gene transcription. At the same time, they are pernicious in intercellular space because they stimulate systemic inflammatory and toxic responses. Myelin basic protein (MBP) is the major protein of the axon myelin–proteolipid sheath. Antibody-abzymes with various catalytic activities are specific features of some autoimmune diseases. IgGs against five individual histones (H2B, H1, H2A, H3, and H4) and MBP were isolated from the blood of experimental autoimmune encephalomyelitis-prone C57BL/6 mice by affinity chromatography. Abzymes corresponding to various stages of EAE development, including spontaneous EAE, myelin oligodendrocyte glycoprotein (MOG)- and DNA-histone complex-accelerated onset, as well as acute and remission stages, were analyzed. IgG-abzymes against MBP and five individual histones showed unusual polyreactivity in complex formation and enzymatic cross-reactivity in the specific hydrolysis of H2B histone. All IgGs against MBP and individual histones in 3-month-old mice (zero time) demonstrated from 4 to 11 different H2B hydrolysis sites. Spontaneous development of EAE during 60 days led to a significant change in the type and number of H2B hydrolysis sites by IgGs against the five histones and MBP. Mouse treatment with MOG and DNA-histone complex changed the type and number of H2B hydrolysis sites compared to zero time. The minimum number (3) of different H2B hydrolysis sites was found for IgGs against H3 20 days after mouse immunization with DNA-histone complex, whereas the maximum number (33) for anti-H2B IgGs was found 60 days after mouse treatment with DNA-histone complex. Overall, this is the first study to demonstrate that at different stages of EAE evolution, IgG-abzymes against five individual histones and MBP could significantly differ in the specific sites and number of H2B hydrolysis sites. Possible reasons for the catalytic cross-reactivity and significant differences in the number and type of histone H2B cleavage sites were analyzed.
CdSSe Nano-Flowers for Ultrasensitive Raman Detection of Antibiotics
Authors: Wang Kangkang , Li You , Wang Honggang , Qian Ziyue , Zhu Xiaokai , Hussain Sabir , Xie Liming
Published: March 2023

Surface-enhanced Raman scattering (SERS) technique is widely used for the highly sensitive detection of trace residues due to its unparalleled signal amplification ability and plays an important role in food safety, environmental monitoring, etc. Herein, CdSSe nano-flowers (CdSSe NFs) are synthesized via the chemical vapor deposition (CVD) method. CdSSe NFs thin film is used as a SERS substrate with an ultralow limit of detection (LOD, 10−14 M), high apparent enhancement factor (EF, 3.62 × 109), and excellent SERS stability (relative standard deviation, RSD = 3.05%) for probe molecules of Rh6G. Further, CdSSe NFs substrate is successfully applied in the sensitive, quantitative, and label-free analysis of ciprofloxacin (CIP) and enrofloxacin (ENR) antibiotics, which exhibit LODs of below 0.5 ppb. This excellent SERS platform may be widely utilized for sensitive life science and environmental sensing.
Cis-Nerolidol Inhibits MAP Kinase and NF-κB Signaling Pathways and Prevents Epithelial Tight Junction Dysfunction in Colon Inflammation: In Vivo and In Vitro Studies
Authors: Raj Vishnu , Venkataraman Balaji , Ojha Shreesh K. , Almarzooqi Saeeda , Subramanian Veedamali S. , Al-Ramadi Basel K. , Adrian Thomas E. , Subramanya Sandeep B.
Published: March 2023

Inflammation of the GI tract leads to compromised epithelial barrier integrity, which increases intestine permeability. A compromised intestinal barrier is a critical event that leads to microbe entry and promotes inflammatory responses. Inflammatory bowel diseases that comprise Crohn’s disease (CD) and ulcerative colitis (UC) show an increase in intestinal permeability. Nerolidol (NED), a naturally occurring sesquiterpene alcohol, has potent anti-inflammatory properties in preclinical models of colon inflammation. In this study, we investigated the effect of NED on MAPKs, NF-κB signaling pathways, and intestine epithelial tight junction physiology using in vivo and in vitro models. The effect of NED on proinflammatory cytokine release and MAPK and NF-κB signaling pathways were evaluated using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophages. Subsequently, the role of NED on MAPKs, NF-κB signaling, and the intestine tight junction integrity were assessed using DSS-induced colitis and LPS-stimulated Caco-2 cell culture models. Our result indicates that NED pre-treatment significantly inhibited proinflammatory cytokine release, expression of proteins involved in MAP kinase, and NF-κB signaling pathways in LPS-stimulated RAW macrophages and DSS-induced colitis. Furthermore, NED treatment significantly decreased FITC-dextran permeability in DSS-induced colitis. NED treatment enhanced tight junction protein expression (claudin-1, 3, 7, and occludin). Time-dependent increases in transepithelial electrical resistance (TEER) measurements reflect the formation of healthy tight junctions in the Caco-2 monolayer. LPS-stimulated Caco-2 showed a significant decrease in TEER. However, NED pre-treatment significantly prevented the fall in TEER measurements, indicating its protective role. In conclusion, NED significantly decreased MAPK and NF-κB signaling pathways and decreased tight junction permeability by enhancing epithelial tight junction protein expression.
Application of Computing as a High-Practicability and -Efficiency Auxiliary Tool in Nanodrugs Discovery
Authors: Xu Ke , Li Shilin , Zhou Yangkai , Gao Xinglong , Mei Jie , Liu Ying
Published: March 2023

Research and development (R&D) of nanodrugs is a long, complex and uncertain process. Since the 1960s, computing has been used as an auxiliary tool in the field of drug discovery. Many cases have proven the practicability and efficiency of computing in drug discovery. Over the past decade, computing, especially model prediction and molecular simulation, has been gradually applied to nanodrug R&D, providing substantive solutions to many problems. Computing has made important contributions to promoting data-driven decision-making and reducing failure rates and time costs in discovery and development of nanodrugs. However, there are still a few articles to examine, and it is necessary to summarize the development of the research direction. In the review, we summarize application of computing in various stages of nanodrug R&D, including physicochemical properties and biological activities prediction, pharmacokinetics analysis, toxicological assessment and other related applications. Moreover, current challenges and future perspectives of the computing methods are also discussed, with a view to help computing become a high-practicability and -efficiency auxiliary tool in nanodrugs discovery and development.
Impact of an Interprofessional Education Activity on Student Knowledge and Attitudes Regarding Patient Safety
Authors: Vyas Deepti , DelNero Tracey , Reece Benjamin
Published: March 2023

This study aimed to measure the impact of an interprofessional education (IPE) activity on student knowledge and attitudes regarding patient safety. Two 4 h IPE activities were designed to provide students with foundational information regarding patient safety. Interprofessional teams discussed the individual curricula and roles/responsibilities of each represented health profession. Teams then served on a mock committee tasked with completing a root cause analysis of a fictitious sentinel event. Students completed a pre/post-quiz and pre/post-attitudes survey to measure knowledge and attitudes. Five months later, students reconvened to serve on a second mock sentinel event committee. Students completed a post-activity survey after the second activity. Four hundred and seven students participated in the first activity, while two hundred and eighty participated in the second activity. Quiz score comparisons revealed improved knowledge, with post-quiz scores being significantly higher. Pre- and post-attitude survey comparisons indicated a significant improvement in participant attitudes towards interprofessional teamwork. Seventy-eight percent of students reported the IPE activity enhanced their ability to “engage other health professions students in shared patient-centered care”. This IPE activity resulted in knowledge and attitude improvement related to patient safety.
Grape Heterogeneity Index: Assessment of Overall Grape Heterogeneity Using an Aggregation of Multiple Indicators
Authors: Armstrong Claire E. J. , Previtali Pietro , Boss Paul K. , Pagay Vinay , Bramley Robert G. V. , Jeffery David W.
Published: March 2023

Uniform grape maturity can be sought by producers to minimise underripe and/or overripe proportions of fruit and limit any undesirable effects on wine quality. Considering that grape heterogeneity is a multifaceted phenomenon, a composite index summarising overall grape heterogeneity was developed to benefit vineyard management and harvest date decisions. A grape heterogeneity index (GHI) was constructed by aggregating the sum of absolute residuals multiplied by the range of values from measurements of total soluble solids, pH, fresh weight, total tannins, absorbance at 520 nm (red colour), 3-isobutyl-2-methoxypyrazine, and malic acid. Management of grape heterogeneity was also studied, using Cabernet Sauvignon grapes grown under four viticultural regimes (normal/low crop load, full/deficit irrigation) during the 2019/2020 and 2020/2021 seasons. Comparisons of GHI scores showed grape variability decreased throughout ripening in both vintages, then significantly increased at the harvest time point in 2020, but plateaued on sample dates nearing the harvest date in 2021. Irrigation and crop load had no effect on grape heterogeneity by the time of harvest in both vintages. Larger vine yield, leaf area index, and pruning weight significantly increased GHI score early in ripening, but no significant relationship was found at the time of harvest. Differences in the Ravaz index, normalised difference vegetation index, and soil electrical conductivity did not significantly change the GHI score.
Host Resistance to Parasitic Plants—Current Knowledge and Future Perspectives
Authors: Albanova Ivanela A. , Zagorchev Lyuben I. , Teofanova Denitsa R. , Odjakova Mariela K. , Kutueva Lyudmila I. , Ashapkin Vasily V.
Published: March 2023

Parasitic flowering plants represent a diverse group of angiosperms, ranging from exotic species with limited distribution to prominent weeds, causing significant yield losses in agricultural crops. The major damage caused by them is related to the extraction of water and nutrients from the host, thus decreasing vegetative growth, flowering, and seed production. Members of the root parasites of the Orobanchaceae family and stem parasites of the genus Cuscuta are among the most aggressive and damaging weeds, affecting both monocotyledonous and dicotyledonous crops worldwide. Their control and eradication are hampered by the extreme seed longevity and persistence in soil, as well as their taxonomic position, which makes it difficult to apply selective herbicides not damaging to the hosts. The selection of resistant cultivars is among the most promising approaches to deal with this matter, although still not widely employed due to limited knowledge of the molecular mechanisms of host resistance and inheritance. The current review aims to summarize the available information on host resistance with a focus on agriculturally important parasitic plants and to outline the future perspectives of resistant crop cultivar selection to battle the global threat of parasitic plants.
Biomass and Leaf Nutrition Contents of Selected Grass and Legume Species in High Altitude Rangelands of Kashmir Himalaya Valley (Jammu & Kashmir), India
Authors: Mugloo Javed A. , Khanday Mehraj ud din , Dar Mehraj ud din , Saleem Ishrat , Alharby Hesham F. , Bamagoos Atif A. , Alghamdi Sameera A. , Abdulmajeed Awatif M. , Kumar Pankaj , Abou Fayssal Sami
Published: March 2023

The yield and nutritional profile of grass and legume species in Kashmir Valley’s rangelands are scantly reported. The study area in this paper included three types of sites (grazed, protected, and seed-sown) divided into three circles: northern, central, and southern Kashmir. From each circle, three districts and three villages per district were selected. Most sites showed higher aboveground biomass (AGB) compared to belowground biomass (BGB), which showed low to moderate effects on biomass. The comparison between northern, central, and southern Kashmir regions revealed that AGB (86.74, 78.62, and 75.22 t. ha−1), BGB (52.04, 51.16, and 50.99 t. ha−1), and total biomass yield (138.78, 129.78, and 126.21 t. ha−1) were the highest in central Kashmir region, followed by southern and northern Kashmir regions, respectively. More precisely, AGB and total biomass yield recorded the highest values in the protected sites of the central Kashmir region, whereas BGB scored the highest value in the protected sites of southern Kashmir region. The maximum yield (12.5 t. ha−1) recorded among prominent grasses was attributed to orchard grass, while the highest crude fiber and crude protein contents (34.2% and 10.4%, respectively), were observed for Agrostis grass. The maximum yield and crude fiber content (25.4 t. ha−1 and 22.7%, respectively), among prominent legumes were recorded for red clover. The highest crude protein content (33.2%) was attributed to white clover. Those findings concluded the successful management of Kashmir rangelands in protected sites, resulting in high biomass yields along with the considerable nutritional value of grasses and legumes.
Conventional vs. Microwave-Assisted Hydrodistillation: Influence on the Chemistry of Sea Fennel Essential Oil and Its By-Products
Authors: Politeo Olivera , Popović Marijana , Veršić Bratinčević Maja , Koceić Petra , Ninčević Runjić Tonka , Mekinić Ivana Generalić
Published: March 2023

The main objectives of this study were to investigate the effects of the applied essential oil (EO) isolation method, conventional hydro-distillation (HD), and microwave-assisted hydro-distillation (MHD) on the chemical profile of sea fennel (Crithmum maritimum L.) essential oil and to investigate the main constituents present in the liquid by-products of EOs isolation (hydrolate and residual wastewater). Headspace-solid phase microextraction (HS-SPME) was used to isolate hydrolate components, while gas chromatography coupled with mass spectrometry (GC-MS) was used to detect and analyse the chemical constituents of the essential oils and hydrolates. The phenolic composition of the wastewater extracts was analysed by high performance liquid chromatography (HPLC). The EO obtained by MHD had a higher yield of limonene and sabinene. The chemical composition of the hydrolates differed from the EO compositions. The content of terpinen-4-ol in the MHD hydrolate was higher, while several compounds were detected in relatively high proportions only in the HD hydrolate. MHD also resulted in a higher phenolic content of the wastewater, where an increase in the concentration of chlorogenic acid was also observed. It can be concluded that the isolation method had a great influence on the profile of sea fennel EOs, especially on their corresponding hydrolates and residual wastewater extracts. Due to their valuable chemical composition, these by-products can be a cost-effective source of bioactive compounds that have great potential for use in various industries.
Application of Polysaccharide-Based Edible Coatings on Fruits and Vegetables: Improvement of Food Quality and Bioactivities
Authors: Cruz-Monterrosa Rosy G. , Rayas-Amor Adolfo A. , González-Reza Ricardo M. , Zambrano-Zaragoza María L. , Aguilar-Toalá José E. , Liceaga Andrea M.
Published: March 2023

Most foods derived from plant origin are very nutritious but highly perishable products. Nowadays, the food industry is focusing on the development of efficient preservation strategies as viable alternatives to traditional packaging and chemical treatments. Hence, polysaccharide-based edible coatings have been proposed because of their properties of controlled release of food additives and the protection of sensitive compounds in coated foods. Thus, this technology has allowed for improving the quality parameters and extends the shelf life of fruits and vegetables through positive effects on enzyme activities, physicochemical characteristics (e.g., color, pH, firmness, weight, soluble solids), microbial load, and nutritional and sensory properties of coated foods. Additionally, some bioactive compounds have been incorporated into polysaccharide-based edible coatings, showing remarkable antioxidant and antimicrobial properties. Thus, polysaccharide-based edible coatings incorporated with bioactive compounds can be used not only as an efficient preservation strategy but also may play a vital role in human health when consumed with the food. The main objective of this review is to provide a comprehensive overview of materials commonly used in the preparation of polysaccharide-based edible coatings, including the main bioactive compounds that can be incorporated into edible coatings, which have shown specific bioactivities.
Mechanical and Thermal Properties of Thermally Conductive Enhanced Paraffin/Gypsum Composites
Authors: Chen Chang , Wang Huan , Wang Yubin , Chen Yanxin , Jiu Shaowu
Published: March 2023

The low thermal conductivity of gypsum-based composites containing phase change materials (PCMs) has limited their application in construction materials. In this study, the mechanical and thermal properties and microstructures of paraffin (PA)/gypsum composites containing iron powder, copper powder, and expanded graphite were characterized by a universal testing machine, thermogravimetry, differential scanning calorimetry, and scanning electron microscopy. The PA/gypsum composites had optimal mechanical properties when the PA content was 20 wt.%. The compressive and flexural strengths were 9.46 MPa and 3.57 MPa, respectively. When the copper powder content increased, the densities and porosities of the PA/gypsum composites containing the copper powder did not largely change. The average density and porosity of the composites were 1.17 g/cm3 and 46%, respectively. The compressive and flexural strengths of the PA/gypsum composite containing 8 wt.% copper powder were optimal. A phase change temperature of 48.8 °C was obtained when 8 wt.% copper powder was added to the PA/gypsum composite. The thermal conductivities of the PA/gypsum composites were lower than those of PA/gypsum composites with 8 wt.% iron powder, 8 wt.% copper powder, or 8 wt.% expanded graphite. Parts of coarse calcium sulfate dihydrate crystals were present at the interfaces between the gypsum and PA, which indicated that the growth of calcium sulfate dihydrate crystals was affected by the copper powder.
PVDF Membrane-Based Dual-Channel Acoustic Sensor Integrating the Fabry–Pérot and Piezoelectric Effects
Authors: Yao Qingkai , Xie Linfang , Guo Xing , Yu Fapeng , Zhao Xian
Published: March 2023

A resonant acoustic wave detector combined with Fabry–Pérot interference (FPI) and piezoelectric (PE) effects based on a polyvinylidene fluoride (PVDF) piezoelectric film was proposed to enhance the ability of the sensor to detect acoustic signals in a specific frequency band. The deformation of circular thin films was indicated by the interference and piezoelectric effects simultaneously, and the noise level was decreased by the real-time convolution of the two-way parallel signal. This study reveals that, at the film’s resonance frequency, the minimum detection limits for the FPI and piezoelectric impacts on acoustic waves are 3.39 μPa/Hz1/2 and 20.8 μPa/Hz1/2, respectively. The convolution result shows that the background noise was reduced by 98.81% concerning the piezoelectric signal, and by 85.21% concerning the FPI signal. The convolution’s signal-to-noise ratio (SNR) was several times greater than the other two signals at 10 mPa. Therefore, this resonance sensor, which the FPI and the piezoelectric effect synergistically enhance, can be applied to scenarios of acoustic wave detection in a specific frequency band and with ultrahigh sensitivity requirements.
Description of Telemark Skiing Technique Using Full Body Inertial Measurement Unit
Authors: Aschenbrenner Piotr , Krawczyński Bartosz , Krawczyński Marcin , Grzywacz Tomasz , Erdmann Włodzimierz
Published: March 2023

Researchers involved in skiing investigations postulate Telemark skiing as an alternative technique to Alpine skiing, which may be associated with lower injury risk. A free heel of the boot, and a boot that enables flexion of the toe, are characteristic features. The aim of this research was to compare three types of turns on Telemark skis, through a biomechanical description of each skiing technique. Seven professional skiers were investigated. Two cameras and the MyoMotion Research Pro system were utilized. Eighteen wireless IMU sensors were mounted on each skier’s body. For every skier, five runs were recorded for each of the three turning techniques. Velocity of run, range of movement, angular velocity in joints, time sequences, and order of initialization of movement were obtained. A higher velocity of skiing was obtained during the parallel (14.2 m/s) and rotational turns (14.9 m/s), compared to a low–high turn (8.9 m/s). A comparison of knee angles, revealed similar minimum (18 and 16 degrees) and maximum (143 and 147 degrees) values achieved during the parallel and rotational techniques, which differed considerably from the low–high technique (27 and 121 degrees, respectively). There were no significant differences in trunk rotation angles. A detailed analysis of the Telemark skiing technique revealed novel information on how turns are executed by well-trained skiers and the impact of different approaches.
A Power Allocation Scheme for MIMO-NOMA and D2D Vehicular Edge Computing Based on Decentralized DRL
Authors: Long Dunxing , Wu Qiong , Fan Qiang , Fan Pingyi , Li Zhengquan , Fan Jing
Published: March 2023

In vehicular edge computing (VEC), some tasks can be processed either locally or on the mobile edge computing (MEC) server at a base station (BS) or a nearby vehicle. In fact, tasks are offloaded or not, based on the status of vehicle-to-infrastructure (V2I) and vehicle-to-vehicle (V2V) communication. In this paper, device-to-device (D2D)-based V2V communication and multiple-input multiple-output and nonorthogonal multiple access (MIMO-NOMA)-based V2I communication are considered. In actual communication scenarios, the channel conditions for MIMO-NOMA-based V2I communication are uncertain, and the task arrival is random, leading to a highly complex environment for VEC systems. To solve this problem, we propose a power allocation scheme based on decentralized deep reinforcement learning (DRL). Since the action space is continuous, we employ the deep deterministic policy gradient (DDPG) algorithm to obtain the optimal policy. Extensive experiments demonstrate that our proposed approach with DRL and DDPG outperforms existing greedy strategies in terms of power consumption and reward.
System for Estimation of Human Anthropometric Parameters Based on Data from Kinect v2 Depth Camera
Authors: Krzeszowski Tomasz , Dziadek Bartosz , França Cíntia , Martins Francisco , Gouveia Élvio Rúbio , Przednowek Krzysztof
Published: March 2023

Anthropometric measurements of the human body are an important problem that affects many aspects of human life. However, anthropometric measurement often requires the application of an appropriate measurement procedure and the use of specialized, sometimes expensive measurement tools. Sometimes the measurement procedure is complicated, time-consuming, and requires properly trained personnel. This study aimed to develop a system for estimating human anthropometric parameters based on a three-dimensional scan of the complete body made with an inexpensive depth camera in the form of the Kinect v2 sensor. The research included 129 men aged 18 to 28. The developed system consists of a rotating platform, a depth sensor (Kinect v2), and a PC computer that was used to record 3D data, and to estimate individual anthropometric parameters. Experimental studies have shown that the precision of the proposed system for a significant part of the parameters is satisfactory. The largest error was found in the waist circumference parameter. The results obtained confirm that this method can be used in anthropometric measurements.
Design and Modeling of a Device Combining Single-Cell Exposure to a Uniform Electrical Field and Simultaneous Characterization via Bioimpedance Spectroscopy
Authors: Bettenfeld Rémi , Claudel Julien , Kourtiche Djilali , Nadi Mustapha , Schlauder Cyril
Published: March 2023

Previous studies have demonstrated the electropermeabilization of cell membranes exposed to an electric field with moderate intensity (<2 V/cm) and a frequency of <100 MHz. Bioimpedance spectroscopy (BIS) is an electrical characterization technique that can be useful in studying this phenomenon because it is already used for electroporation. In this paper, we report a device designed to perform BIS on single cells and expose them to an electric field simultaneously. It also allows cells to be monitored by visualization through a transparent exposure electrode. This device is based on a lab-on-a-chip (LOC) with a microfluidic cell-trapping system and microelectrodes for BIS characterization. We present numerical simulations that support the design of the LOC. We also describe the fabrication of the LOC and the first electrical characterization of its measurement bandwidth. This first test, performed on reference medium with a conductivity in the same order than human cells, confirms that the measurement capabilities of our device are suitable for electrical cells characterization.
State-of-the-Art Room Temperature Operable Zero-Bias Schottky Diode-Based Terahertz Detector Up to 5.56 THz
Authors: Yadav Rahul , Ludwig Florian , Faridi Fahd Rushd , Klopf J. Michael , Roskos Hartmut G. , Preu Sascha , Penirschke Andreas
Published: March 2023

We present the characterization of a Zero-bias Schottky diode-based Terahertz (THz) detector up to 5.56 THz. The detector was operated with both a table-top system until 1.2 THz and at a Free-Electron Laser (FEL) facility at singular frequencies from 1.9 to 5.56 THz. We used two measurement techniques in order to discriminate the sub-ns-scale (via a 20 GHz oscilloscope) and the ms-scale (using the lock-in technique) responsivity. While the lock-in measurements basically contain all rectification effects, the sub-ns-scale detection with the oscilloscope is not sensitive to slow bolometric effects caused by changes of the IV characteristic due to temperature. The noise equivalent power (NEP) is 10 pW/Hz in the frequency range from 0.2 to 0.6 THz and 17 pW/Hz at 1.2 THz and increases to 0.9 μW/Hz at 5.56 THz, which is at the state of the art for room temperature zero-bias Schottky diode-based THz detectors with non-resonant antennas. The voltage and current responsivity of ∼500 kV/W and ∼100 mA/W, respectively, is demonstrated over a frequency range of 0.2 to 1.2 THz with the table-top system.
Influence of Pitch on Surface Dose Distribution and Image Noise of Computed Tomography Scans
Authors: Yamada Kenji , Kawata Yoshiki , Amano Masafumi , Suzuki Hidenobu , Tominaga Masahide , Sasaki Motoharu , Nishiyama Hikaru , Harada Masafumi , Niki Noboru
Published: March 2023

This study evaluated the effect of pitch on 256-slice helical computed tomography (CT) scans. Cylindrical water phantoms (CWP) were measured using axial and helical scans with various pitch values. The surface dose distributions of CWP were measured, and reconstructed images were obtained using filtered back-projection (FBP) and iterative model reconstruction (IMR). The image noise in each reconstructed image was decomposed into a baseline component and another component that varied along the z-axis. The baseline component of the image noise was highest at the center of the reconstructed image and decreased toward the edges. The normalized 2D power spectra for each pitch were almost identically distributed. Furthermore, the ratios of the 2D power spectra for IMR and FBP at different pitch values were obtained. The magnitudes of the components varying along the z-axis were smallest at the center of the reconstructed image and increased toward the edge. The ratios of the 3D power spectra on the fx axis for IMR and FBP at different pitch values were obtained. The results showed that the effect of the pitch was related to the component that varied along the z-axis. Furthermore, the pitch had a smaller effect on IMR than on FBP.
Treatment of the Paretic Hand with a Robotic Glove Combined with Physiotherapy in a Patient Suffering from Traumatic Tetraparesis: A Case Report
Authors: Bressi Federica , Cricenti Laura , Bravi Marco , Pannunzio Fabiana , Cordella Francesca , Lapresa Martina , Miccinilli Sandra , Santacaterina Fabio , Zollo Loredana , Sterzi Silvia , Campagnola Benedetta
Published: March 2023

Background: cervical spinal cord injury leads to loss of upper limb functionality, which causes a decrease in autonomy to perform activities of daily living. The use of robotic technologies in rehabilitation could contribute to improving upper limb functionality and treatment quality. This case report aims to describe the potential of robotic hand treatment with Gloreha Sinfonia, in combination with conventional rehabilitation, in a tetraparetic patient. Material: fifteen rehabilitative sessions were performed. Evaluations were conducted pre-treatment (T0), post-treatment (T1), and at two-months follow-up (T2) based on: the upper-limb range of motion and force assessment, the FMA-UE, the 9-Hole Peg Test (9HPT), and the DASH questionnaire. A virtual reality game-based rating system was used to evaluate the force control and modulation ability. Results: the patient reported greater ability to use hands with less compensation at T1 and T2 assessments. Improvements in clinical scales were reported in both hands at T1, however, at T2 only did the dominant hand show further improvement. Improved grip strength control and modulation ability were reported for T1. However a worsening was found in both hands at T2, significant only for the non-dominant hand. The maximum force exerted increased from T0 to T2 in both hands. Conclusion: hand treatment combining physical therapy and Gloreha Sinfonia seems to have benefits in functionality and dexterity in tetraparetic patient in the short term. Further studies are needed to confirm these findings, to verify long-term results, and to identify the most appropriate modalities of robotic rehabilitation.
Bacterial Colony Phenotyping with Hyperspectral Elastic Light Scattering Patterns
Authors: Doh Iyll-Joon , Zuniga Diana Vanessa Sarria , Shin Sungho , Pruitt Robert E. , Rajwa Bartek , Robinson J. Paul , Bae Euiwon
Published: March 2023

The elastic light-scatter (ELS) technique, which detects and discriminates microbial organisms based on the light-scatter pattern of their colonies, has demonstrated excellent classification accuracy in pathogen screening tasks. The implementation of the multispectral approach has brought further advantages and motivated the design and validation of a hyperspectral elastic light-scatter phenotyping instrument (HESPI). The newly developed instrument consists of a supercontinuum (SC) laser and an acousto-optic tunable filter (AOTF). The use of these two components provided a broad spectrum of excitation light and a rapid selection of the wavelength of interest, which enables the collection of multiple spectral patterns for each colony instead of relying on single band analysis. The performance was validated by classifying microflora of green-leafed vegetables using the hyperspectral ELS patterns of the bacterial colonies. The accuracy ranged from 88.7% to 93.2% when the classification was performed with the scattering pattern created at a wavelength within the 473–709 nm region. When all of the hyperspectral ELS patterns were used, owing to the vastly increased size of the data, feature reduction and selection algorithms were utilized to enhance the robustness and ultimately lessen the complexity of the data collection. A new classification model with the feature reduction process improved the overall classification rate to 95.9%.
Assessment of Passive Upper Limb Stiffness and Its Function in Post-Stroke Individuals Wearing an Inertial Sensor during the Pendulum Test
Authors: de Lima Milene Soares Nogueira , dos Santos Couto Paz Clarissa Cardoso , Ribeiro Thais Gontijo , Fachin-Martins Emerson
Published: March 2023

This article proposes the evaluation of the passive movement of the affected elbow during the pendulum test in people with stroke and its correlation with the main clinical scales (Modified Ashworth Scale, Motor Activity Log, and Fulg Meyer). An inertial sensor was attached to the forearm of seven subjects, who then passively flexed and extended the elbow. Joint angles and variables that indicate viscoelastic properties, stiffness (K), damping (B), E1 amp, F1 amp, and relaxation indices were collected. The results show that the FM scale is significantly correlated with the natural frequency (p = 0.024). The MAL amount-of-use score correlates with the natural frequency (p = 0.024). The variables E1 amp, F1 amp, RI, and ERI are not correlated with the clinical scales, but they correlate with each other; the variable E1 amp correlates with F1 amp (p = 0.024) and RI (p = 0.024), while F1 amp correlates with ERI (p = 0.024). There was also a correlation between the natural frequency and K (r = 0.96, p = 0.003). Non-linear results were found for the properties of the elbow joint during the pendulum test, which may be due to the presence of neural and non-neural factors. These results may serve as a reference for future studies if alternative scales do not provide an accurate reflection.
A Lightweight Double-Stage Scheme to Identify Malicious DNS over HTTPS Traffic Using a Hybrid Learning Approach
Authors: Abu Al-Haija Qasem , Alohaly Manar , Odeh Ammar
Published: March 2023

The Domain Name System (DNS) protocol essentially translates domain names to IP addresses, enabling browsers to load and utilize Internet resources. Despite its major role, DNS is vulnerable to various security loopholes that attackers have continually abused. Therefore, delivering secure DNS traffic has become challenging since attackers use advanced and fast malicious information-stealing approaches. To overcome DNS vulnerabilities, the DNS over HTTPS (DoH) protocol was introduced to improve the security of the DNS protocol by encrypting the DNS traffic and communicating it over a covert network channel. This paper proposes a lightweight, double-stage scheme to identify malicious DoH traffic using a hybrid learning approach. The system comprises two layers. At the first layer, the traffic is examined using random fine trees (RF) and identified as DoH traffic or non-DoH traffic. At the second layer, the DoH traffic is further investigated using Adaboost trees (ADT) and identified as benign DoH or malicious DoH. Specifically, the proposed system is lightweight since it works with the least number of features (using only six out of thirty-three features) selected using principal component analysis (PCA) and minimizes the number of samples produced using a random under-sampling (RUS) approach. The experiential evaluation reported a high-performance system with a predictive accuracy of 99.4% and 100% and a predictive overhead of 0.83 µs and 2.27 µs for layer one and layer two, respectively. Hence, the reported results are superior and surpass existing models, given that our proposed model uses only 18% of the feature set and 17% of the sample set, distributed in balanced classes.
Multi-Sensor Medical-Image Fusion Technique Based on Embedding Bilateral Filter in Least Squares and Salient Detection
Authors: Li Jiangwei , Han Dingan , Wang Xiaopan , Yi Peng , Yan Liang , Li Xiaosong
Published: March 2023

A multi-sensor medical-image fusion technique, which integrates useful information from different single-modal images of the same tissue and provides a fused image that is more comprehensive and objective than a single-source image, is becoming an increasingly important technique in clinical diagnosis and treatment planning. The salient information in medical images often visually describes the tissue. To effectively embed salient information in the fused image, a multi-sensor medical image fusion method is proposed based on an embedding bilateral filter in least squares and salient detection via a deformed smoothness constraint. First, source images are decomposed into base and detail layers using a bilateral filter in least squares. Then, the detail layers are treated as superpositions of salient regions and background information; a fusion rule for this layer based on the deformed smoothness constraint and guided filtering was designed to successfully conserve the salient structure and detail information of the source images. A base-layer fusion rule based on modified Laplace energy and local energy is proposed to preserve the energy information of these source images. The experimental results demonstrate that the proposed method outperformed nine state-of-the-art methods in both subjective and objective quality assessments on the Harvard Medical School dataset.
Vulnerability Analysis and Security Assessment of Secure Keyboard Software to Prevent PS/2 Interface Keyboard Sniffing
Authors: Lee Kyungroul , Yim Kangbin
Published: March 2023

Online security threats have arisen through Internet banking hacking cases, and highly sensitive user information such as the ID, password, account number, and account password that is used for online payments has become vulnerable. Many security companies have therefore researched protection methods regarding keyboard-entered data for the introduction of defense techniques. Recently, keyboard security issues have arisen due to the production of new malicious codes by attackers who have combined the existing attack techniques with new attack techniques; however, a keyboard security assessment is insufficient here. The research motivation is to serve more secure user authentication methods by evaluating the security of information input from the keyboard device for the user authentication, including Internet banking service. If the authentication information input from the keyboard device is exposed during user authentication, attackers can attempt to illegal login or, worst, steal the victim’s money. Accordingly, in this paper, the existing and the new keyboard-attack techniques that are known are surveyed, and the results are used as the basis for the implementation of sample malicious codes to verify both a security analysis and an assessment of secure keyboard software. As a result of the experiment, if the resend command utilization attack technique is used, 7 out of 10 companies’ products expose keyboard information, and only 1 company’s products detect it. The fundamental reason for these vulnerabilities is that the hardware chip related to the PS/2 interface keyboard does not provide security facilities. Therefore, since keyboard data exposure does not be prevented only by software, it is required to develop a hardware chip that provides security facilities.
Animals Other than Human Animals and Their Claim to Equal Consideration in Coronavirus and Criminological Study: Examining Harm to Domesticated Dogs during COVID-19 in the UK
Authors: Gregory Rebekah Kim
Published: March 2023

While the financial and social support afforded to United Kingdon (UK) ‘animals other than human animals’ (AOTHAs) welfare charities, such as the RSPCA and Dogs Trust, could suggest that they are valued intrinsically within Western society criminological texts largely omit them from recognition, with some important exceptions, see non-speciesist criminology. Most human animals likely do not want to directly or indirectly harm AOTHAs and even value relationships with “companion” AOTHAs. Regardless, AOTHAs have been victimized throughout history and continue to be. This article examines harm to AOTHAs in the context of the global COVID-19 pandemic to argue that abuse proliferates where harmful subjectivities are generated by society’s acceptance of (1) the anthropocentric culture, and (2) when humanity values their individual advancement within the competitive consumer culture. Companion dogs were specifically focused upon within this article due to their so called close emotional and physical proximity to human animals, with proximity meaning that they were directly impacted by the lockdown measures implemented. The coronavirus pandemic was addressed by governments throughout the world by initiating an array of social restrictions. Because of these social restrictions, millions of individuals within England, and in countries such as the United States of America (USA), decided to adopt or purchase dogs for a variety of reasons, including to help them mitigate feelings of isolation and loneliness and to provide them with an excuse to participate in outdoor exercise. In order to determine the impact that the coronavirus pandemic has had upon the plight of domesticated companion dogs within England, semi-structured interviews, document analyses, and observation research were undertaken. The initial analysis of data presented here suggests that the coronavirus pandemic threatened the wellbeing of dogs within England, with their reproductive, physical, medical, and psychological wellbeing being put at risk.
Proportions of Wood Members in Japanese Traditional Architecture—A Comparison of the Kiwari-Sho and Measurements of Building Remains
Authors: Kim Byungjin
Published: March 2023

Since written reference works on ancient design techniques are still extant, it is possible to gain an understanding of the development of these designs. In particular, we are able to investigate dimensional standards used for wood members using the Kiwari (木割: a method of determining the dimensions of all wood members using a proportion determined based on the diameter of the columns) in Kiwari-sho (木割書: a manual of dimensional standards for wood members). The objective of this study was to confirm whether the design techniques that have been collected under the term “dimensional standards for building (木割術: Kiwari-jutsu)” were actually utilized in the construction of buildings and whether they are a sustainable technology. This study will provide an understanding of the correlation between the techniques used and the dimensions of the remains of structures putatively built using such techniques. Furthermore, based on this analysis, this study will be useful in both quantifying the influence that dimensional standards techniques had on buildings at the time of their construction and as reference material that can be used when restoring the remains of cultural properties.
Awareness and Profiling of High-Risk Asbestos Exposure Groups in Australia
Authors: Khamhing Katrina , McArdle Shane , Ross Justine
Published: March 2023

The increase in home improvement activity during the COVID-19 pandemic gave rise to concerns of increased asbestos exposure risk. This paper describes high-risk asbestos exposure groups based on current home improvement trends in Australia. A series of quantitative and qualitative studies were commissioned to better understand the attitudes, motivations, and behaviours of home improvers in Australia. In 2021, two in three Australian adults were inclined to undertake home improvement projects—big or small—with or without professional help, underscoring the importance of improving the asbestos safety knowledge and capacity of this cohort. The studies commissioned across 2020 and 2021 provide a deep analysis into this cohort, defining who they are and the segments that make up home improvers, their behaviours, and their asbestos awareness and attitudes. This knowledge enables the development and implementation of a range of targeted campaigns to increase asbestos awareness and prevent potential exposure to asbestos fibres.
Placemaking in the Periphery: Leveraging Liminoid Spaces for Host Promotions and Experience Creation at the Japan 2019 Rugby World Cup
Authors: Hayes Christopher J.
Published: March 2023

This research explores the concept of placemaking in the context of sports events tourism, using the case study of the 2019 Rugby World Cup hosted in Japan. The study investigates how host cities used liminoid spaces between transport hubs and stadiums to create a festive atmosphere and articulate the identity of the place itself. Employing a novel ethnographic methodology centred around walking and participatory methods, the researcher occupied a dual tourist-researcher role, immersing himself in the liminoid space. Findings suggest that the use of such spaces was innovative and successful in creating a sense of place and developing a festive atmosphere through which visitors moved. However, the study acknowledges that these strategies may not be applicable to all sports events and that the direct experiences of visitors through ethnographic methods do not allow for empirical claims about the success of strategies or their longitudinal effects. Nevertheless, the study highlights the potential of placemaking in the periphery of sports events to enhance the visitor experience and promote the identity of the host destination.