bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Rootstocks on Blade Nutritional Content of Two Minority Grapevine Varieties Cultivated under Hyper-Arid Conditions in Northern Chile
Authors: Verdugo-Vásquez Nicolás , Gutiérrez-Gamboa Gastón , Villalobos-Soublett Emilio , Zurita-Silva Andrés
Published: February 2021

In the 90s, as in other countries, transformation of Chilean viticulture brought about the introduction and spread of European grapevine varieties which has resulted in a massive loss of minor local and autochthonous grapevine varieties traditionally grown in several wine growing regions. Fortunately, in recent years, autochthonous and minority varieties have been revalued due to their high tolerance to pests and diseases and because of their adaptation to thermal and water stress triggered by global warming. In this study, we assessed the nutritional status of two autochthonous grapevines grafted onto four different rootstocks under the hyper-arid climatic conditions of Northern Chile over three consecutive seasons. The results showed that R32 rootstock induced high N, P, Ca, Mg and Mn levels in blades compared to Harmony rootstock. R32 rootstock and to a lesser extent, 1103 Paulsen and 140 Ruggeri rootstocks kept balanced levels of nutrients in blades collected from Moscatel Amarilla and Moscatel Negra grapevine varieties. Additionally, Harmony presented slight nutritional imbalance compared to the rest of studied rootstocks due to its low absorption of Mg, Mn, Ca and P, and its high K absorption, which was exacerbated under warm weather and salinity soil conditions. These results may provide a basis for specific cultivar/rootstock/site combinations, a nutritional guide for the viticulturists of Northern Chile, and options to diversify their production favoring the use of minority and autochthonous varieties that adapt well to hyper-arid conditions of Northern Chile.
Human Placental NADPH Oxidase Mediates sFlt-1 and PlGF Secretion in Early Pregnancy: Exploration of the TGF-β1/p38 MAPK Pathways
Authors: Hernandez Isabelle , Chissey Audrey , Guibourdenche Jean , Atasoy Roger , Coumoul Xavier , Fournier Thierry , Beaudeux Jean-Louis , Zerrad-Saadi Amal
Published: February 2021

Preeclampsia, a hypertensive disorder occurring during pregnancy, is characterized by excessive oxidative stress and trophoblast dysfunction with dysregulation of soluble Fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF) production. Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase (Nox) is the major source of placental superoxide in early pregnancy and its activation with the subsequent formation of superoxide has been demonstrated for various agents including Transforming Growth Factor beta-1 (TGF-β1), a well-known p38 MAPK pathway activator. However, the bridge between Nox and sFlt-1 remains unknown. The purpose of this study was to explore the possible signaling pathway of TGF-β1/Nox/p38 induced sFlt-1 production in human chorionic villi (CV). Methods: Human chorionic villi from first trimester placenta (7–9 Gestational Weeks (GW)) were treated with TGF-β1 or preincubated with p38 inhibitor, SB203580. For NADPH oxidase inhibition, CV were treated with diphenyleneiodonium (DPI). The protein levels of phospho-p38, p38, phospho-Mothers Against Decapentaplegic homolog 2 (SMAD2), and SMAD2 were detected by Western blot. The secretion of sFlt-1 and PlGF by chorionic villi were measured with Electrochemiluminescence Immunologic Assays, and NADPH oxidase activity was monitored by lucigenin method. Results: We demonstrate for the first time that NADPH oxidase is involved in sFlt-1 and PlGF secretion in first trimester chorionic villi. Indeed, the inhibition of Nox by DPI decreases sFlt-1, and increases PlGF secretions. We also demonstrate the involvement of p38 MAPK in sFlt-1 secretion and Nox activation as blocking the p38 MAPK phosphorylation decreases both sFlt-1 secretion and superoxide production. Nevertheless, TGF-β1-mediated p38 activation do not seem to be involved in regulation of the first trimester placental angiogenic balance and no crosstalk was found between SMAD2 and p38 MAPK pathways. Conclusions: Thus, the placental NADPH oxidase play a major role in mediating the signal transduction cascade of sFlt-1 production. Furthermore, we highlight for the first time the involvement of p38 activation in first trimester placental Nox activity.
Differences between Professional and Amateur Cyclists in Endogenous Antioxidant System Profile
Authors: Martínez-Noguera Francisco Javier , Alcaraz Pedro E. , Ortolano-Ríos Raquel , Dufour Stéphane P. , Marín-Pagán Cristian
Published: February 2021

Currently, no studies have examined the differences in endogenous antioxidant enzymes in professional and amateur cyclists and how these can influence sports performance. The aim of this study was to identify differences in endogenous antioxidants enzymes and hemogram between competitive levels of cycling and to see if differences found in these parameters could explain differences in performance. A comparative trial was carried out with 11 professional (PRO) and 15 amateur (AMA) cyclists. All cyclists performed an endogenous antioxidants analysis in the fasted state (visit 1) and an incremental test until exhaustion (visit 2). Higher values in catalase (CAT), oxidized glutathione (GSSG) and GSSG/GSH ratio and lower values in superoxide dismutase (SOD) were found in PRO compared to AMA (p < 0.05). Furthermore, an inverse correlation was found between power produced at ventilation thresholds 1 and 2 and GSSG/GSH (r = −0.657 and r = −0.635; p < 0.05, respectively) in PRO. Therefore, there is no well-defined endogenous antioxidant enzyme profile between the two competitive levels of cyclists. However, there was a relationship between GSSG/GSH ratio levels and moderate and submaximal exercise performance in the PRO cohort.
Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial
Authors: da Silva Madelaine Torres , de Almeida Barros Mourão Carlos Fernando , Mello-Machado Rafael Coutinho , Montemezzi Pietro , de Lima Barbosa Renata , Sartoretto Suelen Cristina , Leite Paulo Emílio Correa , Javid Kayvon , Kawase Tomoyuki , Alves Gutemberg Gomes , Calasans-Maia Mônica Diuana
Published: February 2021

This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (p < 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (p < 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (ρ = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb.
The Effect of Different Cleaning Methods on Needles for Assessing the Atmospheric Heavy Metal Retention Capacity of Three Coniferous Trees
Authors: Liu Tong , Zhang Mei , Wang Yihan , Tang Lili , Wang Kefan , Shi Fuchen
Published: February 2021

Urban air pollution has received increasing attention in recent years. To investigate the interaction between several heavy metal elements and the degree of atmospheric pollution, the leaves of three evergreen tree species—Chinese juniper (Juniperus chinensis L.), dragon juniper (Juniperus chinensis L. ‘Kaizuca’), and cedar (Cedrus deodara (Roxb. ex D.Don) G.Don)—were collected from main road intersections in the urban area of Tianjin, China. Two different treatments—water washing (WW) and ethanol washing (EW)—were used, and the contents of Cu, Mn, Cd, and Zn were measured in both washed and unwashed (UW) leaves. It was found that the heavy metal contents within Chinese juniper and dragon juniper were ranked as Mn > Zn > Cu > Cd, and the metal accumulation index (MAI) value was higher for dragon juniper. For the three plants, water washed off 5.36% to 58.58% of the total heavy metals in the needles, while ethanol washed off 16.08% to 71.60% of the total. Both washes were more effective for Cu, Zn, and Cd, and especially for the element Cd. Ethanol could clean off 38.64% to 71.60% of the total Cd from the leaves. Ethanol had a better elution effect compared to water, and the trend of the Cd content in the leaves of the three plants showed a change after the use of different washing methods, which suggests that the water washing may have masked the real difference.
Industrial Dust Explosions. A Brief Review
Authors: Eckhoff Rolf K. , Li Gang
Published: February 2021

This paper first addresses the question: what is a dust explosion? Afterwards, some specific issues are briefly reviewed: materials that can give dust explosions, factors influencing ignitability and explosibility of dust clouds, the combustion of dust clouds in air, ignition sources that can initiate dust explosions, primary and secondary dust explosions, dust flash fires, explosions of “hybrid mixtures”, and detonation of dust clouds. Subsequently, measures for dust explosion prevention and mitigation are reviewed. The next section presents the case history of an industrial dust explosion catastrophe in China in 2014. In the final section, a brief review is given of some current research issues that are related to the prevention and mitigation of dust explosions. There is a constant need for further research and development in all the areas elucidated in the paper.
Imaging the Human Thyroid Using Three-Dimensional Diffuse Optical Tomography: A Preliminary Study
Authors: Mimura Tetsuya , Okawa Shinpei , Kawaguchi Hiroshi , Tanikawa Yukari , Hoshi Yoko
Published: February 2021

Thyroid cancer is usually diagnosed by ultrasound imaging and fine-needle aspiration biopsy. However, diagnosis of follicular thyroid carcinomas (FTC) is difficult because FTC lacks nuclear atypia and a consensus on histological interpretation. Diffuse optical tomography (DOT) offers the potential to diagnose FTC because it can measure tumor hypoxia, while image reconstruction of the thyroid is still challenging mainly due to the complex anatomical features of the neck. In this study, we attempted to solve this issue by creating a finite element model of the human neck excluding the trachea (a void region). By reconstruction of the absorption coefficients at three wavelengths, 3D tissue oxygen saturation maps of the human thyroid are obtained for the first time by DOT.
The Use of Electroactive Halophilic Bacteria for Improvements and Advancements in Environmental High Saline Biosensing
Authors: Gaffney Erin M. , Simoska Olja , Minteer Shelley D.
Published: February 2021

Halophilic bacteria are remarkable organisms that have evolved strategies to survive in high saline concentrations. These bacteria offer many advances for microbial-based biotechnologies and are commonly used for industrial processes such as compatible solute synthesis, biofuel production, and other microbial processes that occur in high saline environments. Using halophilic bacteria in electrochemical systems offers enhanced stability and applications in extreme environments where common electroactive microorganisms would not survive. Incorporating halophilic bacteria into microbial fuel cells has become of particular interest for renewable energy generation and self-powered biosensing since many wastewaters can contain fluctuating and high saline concentrations. In this perspective, we highlight the evolutionary mechanisms of halophilic microorganisms, review their application in microbial electrochemical sensing, and offer future perspectives and directions in using halophilic electroactive microorganisms for high saline biosensing.
Systemic Issues Influencing Technical Certainty in Social Housing Programmes in a Small Island Developing State
Authors: Chadee Aaron , Ray Indrajit , Chadee Xsitaaz
Published: February 2021

Underestimating costs in major public sector social infrastructure development projects is a highly contested and problematic issue. Several gaps exist in the identification of root causes of cost overruns. Behavioural science academics advocate political and psychological explanations as root causes for cost overruns against technical factors, such as errors and omissions by engineers, practitioners and scientists. Most studies in this field primarily concentrate on developed countries. Although some studies have been undertaken in developing countries, very little attention is given to the Small Island Developing States (SIDS). This paper presents a case study of public sector social housing construction programmes in a Caribbean SIDS to further understand the root causes of cost overruns. Primary and secondary data spanning across two different political cycles were collected to test the concepts of whether political influences or technical influences are the true root causes of cost overruns. It was found that political explanations based on the psycho-strategic concept are the leading sources of cost risks on the final estimated contract price. This study strengthens the argument and discusses how strategic decisions emanating from the political directorate outweigh and influence informed technical decisions formulated during the planning stages.
Clinical CYP2D6 Genotyping to Personalize Adjuvant Tamoxifen Treatment in ER-Positive Breast Cancer Patients: Current Status of a Controversy
Authors: Mulder Tessa A. M. , de With Mirjam , del Re Marzia , Danesi Romano , Mathijssen Ron H. J. , van Schaik Ron H. N.
Published: February 2021

Tamoxifen is a major option for adjuvant endocrine treatment in estrogen receptor (ER) positive breast cancer patients. The conversion of the prodrug tamoxifen into the most active metabolite endoxifen is mainly catalyzed by the enzyme cytochrome P450 2D6 (CYP2D6). Genetic variation in the CYP2D6 gene leads to altered enzyme activity, which influences endoxifen formation and thereby potentially therapy outcome. The association between genetically compromised CYP2D6 activity and low endoxifen plasma concentrations is generally accepted, and it was shown that tamoxifen dose increments in compromised patients resulted in higher endoxifen concentrations. However, the correlation between CYP2D6 genotype and clinical outcome is still under debate. This has led to genotype-based tamoxifen dosing recommendations by the Clinical Pharmacogenetic Implementation Consortium (CPIC) in 2018, whereas in 2019, the European Society of Medical Oncology (ESMO) discouraged the use of CYP2D6 genotyping in clinical practice for tamoxifen therapy. This paper describes the latest developments on CYP2D6 genotyping in relation to endoxifen plasma concentrations and tamoxifen-related clinical outcome. Therefore, we focused on Pharmacogenetic publications from 2018 (CPIC publication) to 2021 in order to shed a light on the current status of this debate.
Scrutinizing Mechanisms of the ‘Obesity Paradox in Sepsis’: Obesity Is Accompanied by Diminished Formation of Neutrophil Extracellular Traps (NETs) Due to Restricted Neutrophil–Platelet Interactions
Authors: Cichon Iwona , Ortmann Weronika , Santocki Michal , Opydo-Chanek Malgorzata , Kolaczkowska Elzbieta
Published: February 2021

Systemic inflammation is a detrimental condition associated with high mortality. However, obese individuals seem to have higher chances of surviving sepsis. To elucidate what immunological differences exist between obese and lean individuals we studied the course of endotoxemia in mice fed high-fat diet (HFD) and ob/ob animals. Intravital microscopy revealed that neutrophil extracellular trap (NET) formation in liver vasculature is negligible in obese mice in sharp contrast to their lean counterparts (ND). Unlike in lean individuals, neutrophil influx is not driven by leptin or interleukin 33 (IL-33), nor occurs via a chemokine receptor CXCR2. In obese mice less platelets interact with neutrophils forming less aggregates. Platelets transfer from ND to HFD mice partially restores NET formation, and even further so upon P-selectin blockage on them. The study reveals that in obesity the overexaggerated inflammation and NET formation are limited during sepsis due to dysfunctional platelets suggesting their targeting as a therapeutic tool in systemic inflammation.
Particle Coherent Structures in Confined Oscillatory Switching Centrifugation
Authors: Romanò Francesco
Published: February 2021

A small spherical rigid particle in a cylindrical cavity is considered. The harmonic rotation of the cavity wall drives the background flow characterized by the Strouhal number Str, assumed as the first parameter of our investigation. The particle immersed in the flow (assumed Stokesian) has a Stokes number St=1 and a particle-to-fluid density ratio ϱ which is assumed as the second parameter of this study. Building on the theoretical understanding of the recently discovered oscillatory switching centrifugation for inertial particles in unbounded flows, we investigate the effect of a confinement. For the first time we study how the presence of a wall affects the particle trajectory in oscillatory switching centrifugation dynamics. The emergence of two qualitatively different particle attractors is characterized for particles centrifuged towards the cavity wall. The implication of two such classes of attractors is discussed focusing on the application to microfluidics. We propose some strategies for exploiting the confined oscillatory switching centrifugation for selective particle segregation and for the enhancement of particle interaction events.
Role of Albuminuria in Detecting Cardio-Renal Risk and Outcome in Diabetic Subjects
Authors: Pafundi Pia Clara , Garofalo Carlo , Galiero Raffaele , Borrelli Silvio , Caturano Alfredo , Rinaldi Luca , Provenzano Michele , Salvatore Teresa , De Nicola Luca , Minutolo Roberto , Sasso Ferdinando Carlo
Published: February 2021

The clinical significance of albuminuria in diabetic subjects and the impact of its reduction on the main cardiorenal outcomes by different drug classes are among the most interesting research focuses of recent years. Although nephrologists and cardiologists have been paying attention to the study of proteinuria for years, currently among diabetics, increased urine albumin excretion ascertains the highest cardio-renal risk. In fact, diabetes is a condition by itself associated with a high-risk of both micro/macrovascular complications. Moreover, proteinuria reduction in diabetic subjects by several treatments lowers both renal and cardiovascular disease progression. The 2019 joint ESC-EASD guidelines on diabetes, prediabetes and cardiovascular (CV) disease assign to proteinuria a crucial role in defining CV risk level in the diabetic patient. In fact, proteinuria by itself allows the diabetic patient to be staged at very high CV risk, thus affecting the choice of anti-hyperglycemic drug class. The purpose of this review is to present a clear update on the role of albuminuria as a cardio-renal risk marker, starting from pathophysiological mechanisms in support of this role. Besides this, we will show the prognostic value in observational studies, as well as randomized clinical trials (RCTs) demonstrating the potential improvement of cardio-renal outcomes in diabetic patients by reducing proteinuria.
Unraveling an Unusual Phenocopy of Hypertrophic Cardiomyopathy: MELAS Syndrome
Authors: Reid Anna B. , Venetucci Luigi , Schmitt Matthias , Nucifora Gaetano
Published: February 2021

The mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome is an uncommon cause of cardiac hypertrophy, fibrosis, and dysfunction. It shares similar features to numerous other causes of left ventricular hypertrophy, and therefore, because of its rarity, may not be immediately considered as a diagnosis. Prompt recognition of clinical and cardiac imaging features may expedite diagnosis and management. We report the case of a 38-year-old man admitted with neurological symptoms and in whom the diagnostic workup led to the diagnosis of MELAS syndrome with cardiac involvement.
Mathematical Beliefs Held by Costa Rican Pre-Service Teachers and Teacher Educators
Authors: Alfaro Víquez Helen , Joutsenlahti Jorma
Published: February 2021

Beliefs have been conceived as a hidden variable in mathematics education. It is important to know teachers’ beliefs as they can inform the way that teachers teach mathematics, make decisions in the classroom, and form opinions about the abilities of students. In Costa Rica, studies about beliefs have been conducted with in-service teachers, but there is no research on pre-service teachers and the beliefs they bring to the classroom from their teacher education programs (TEPs). This research aims to describe the beliefs held by 76 pre-service teachers and 19 teacher educators from four Costa Rican public universities, using the Teacher Education and Development Study in Mathematics (TEDS-M) questionnaire. The results suggest that both pre-service teachers and teacher educators believe in a constructivist orientation focused on the learner. Both groups support the view of mathematics as a process of inquiry and active learning and agree that mathematical skills are not fixed or associated with gender or culture. In the literature, the beliefs manifested by the participants are associated with positive results regarding student outcomes and teaching practices. Therefore, policymakers should be concerned with providing environments that allow and encourage teachers to continue with these belief orientations when they start teaching.
A Case Study on Data Mining Application in a Virtual Power Plant: Cluster Analysis of Power Quality Measurements
Authors: Jasiński Michał , Sikorski Tomasz , Kaczorowska Dominika , Rezmer Jacek , Suresh Vishnu , Leonowicz Zbigniew , Kostyła Paweł , Szymańda Jarosław , Janik Przemysław , Bieńkowski Jacek , Prus Przemysław
Published: February 2021

One of the recent trends that concern renewable energy sources and energy storage systems is the concept of virtual power plants (VPP). The majority of research now focuses on analyzing case studies of VPP in different issues. This article presents the investigation that is based on a real VPP. That VPP operates in Poland and consists of hydropower plants (HPP), as well as energy storage systems (ESS). For specific analysis, cluster analysis, as a representative technique of data mining, was selected for power quality (PQ) issues. The used data represents 26 weeks of PQ multipoint synchronic measurements for 5 related to VPP points. The investigation discusses different input databases for cluster analysis. Moreover, as an extension to using classical PQ parameters as an input, the application of the global index was proposed. This enables the reduction of the size of the input database with maintaining the data features for cluster analysis. Moreover, the problem of the optimal number of cluster selection is discussed. Finally, the assessment of clustering results was performed to assess the VPP impact on PQ level.
Estimation of Self-Sufficiency Rate in Detached Houses Using Home Energy Management System Data
Authors: Oota Masato , Iwafune Yumiko , Ooka Ryozo
Published: February 2021

Japan’s energy consumption in 2018 was about 2.5 times that in 1975, with the increase in the household sector being the largest at 28%. Most of primary energy is still fossil fuel, and it is urgent to reduce energy consumption in the household sector. The purpose of this paper was to identify ways to reduce household energy consumption without compromising the quality of life in residence. However, the reduction methods vary by region, building specifications, household type, equipment specifications, season, and weather. The value of this paper is based on a systematic analysis of home energy management systems (HEMS) data from about 50,000 households under various conditions. We are analyzing ways to reduce energy consumption. Few studies have analyzed this much back-up data, which is likely to lead to a reduction in CO2 emissions across the household sector. To explore ways to reduce energy consumption in this sector, the company has introduced and provided services for home energy management systems (HEMS) since 2011 and is currently collecting HEMS data for up to 50,000 households. In order to grasp the actual state of energy consumption in each household, HEMS data are systematically analyzed, necessary conditions for energy reduction and self-sufficiency rate (SSR) improvement are analyzed, and energy consumption under certain conditions is estimated using storage batteries (SB) and heat pump water heaters (HPWH). In addition, energy consumption was investigated by actual measurement and simulation for several hundred households. Since power generation and consumption vary greatly depending on the region, building specifications, household type, equipment specifications, season, weather, etc., it is necessary to analyze these factors systematically. As a conclusion, in order to improve SSR, it is necessary to (1) reduce surplus power consumption and energy consumption of heat pump water heaters (HPWHs), (2) increase solar power generation, and (3) increase the size of SB. This study contributes to the spread of advanced housing and the reduction of CO2 emissions in the household sector.
Bigger Is Not Always Better: Review of Small Wind in Brazil
Authors: Araújo Fábio Ricardo Procópio de , Pereira Marcio Giannini , Freitas Marcos Aurélio Vasconcelos , da Silva Neilton Fidelis , Dantas Eduardo Janser de Azevedo
Published: February 2021

This century registers a significant expansion in the wind power market. However, the vast majority of these investments are concentrated in large wind turbines. The century begins with an installed capacity of about 20 GW, which reaches 650 GW in 2019. On the other hand, it is important to highlight that small wind turbines have not followed this virtuous path, a fact that is evident in Brazil’s reality. In this context, the article aims at evaluating the current situation of the wind energy market for Small Wind Turbines in Brazil (SWT) and its future perspectives, identifying the main characteristics of the sector, its challenges, and opportunities. It is an exploratory–explanatory research study that investigates the theme, generating knowledge that turns to practical application, as it seeks answers to solve local interests. This methodological approach provides objective evidence that the production of knowledge about the use of SWT in Brazil still remains embryonic, shaded by the impressive progress registered by the major wind farms in the country, despite all the potential and socioeconomic and environmental attractions. This fact credits the revision research with an innovative role in the apprehension of knowledge related to the development of SWT in Brazil.
Multi-Criteria Fuzzy Evaluation of the Planned Offshore Wind Farm Investments in Poland
Authors: Ziemba Paweł
Published: February 2021

In recent years, the dynamic development of renewable energy has been visible all over the world, including Poland. Wind energy is one of the most used renewable energy sources. In Poland, by 2030, it is planned to commission at least six offshore wind farms with a total capacity of 3.8 GW. It is estimated that these investments will increase Poland’s GDP by approximately PLN 60 billion and increase tax revenues by PLN 15 billion. Therefore, they could be a strong stimulus for the development of the Polish economy and may be of great importance in recovering from the crisis caused by the economic constraints related to the COVID-19 pandemic. The aim of the article is a multi-criteria evaluation of the investments planned in Poland in offshore wind farms and identification of potentially the most economically effective investments. To account for the uncertainty in this decision problem, a modified fuzzy Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method was used and a comprehensive sensitivity analysis was performed. As a result of the research, a ranking of the considered projects was constructed and the most preferred investments were identified. Moreover, it has been shown that all the investments considered are justified and recommended.
Lithium-Ion Capacitors: A Review of Design and Active Materials
Authors: Lamb Jacob J. , Burheim Odne S.
Published: February 2021

Lithium-ion capacitors (LICs) have gained significant attention in recent years for their increased energy density without altering their power density. LICs achieve higher capacitance than traditional supercapacitors due to their hybrid battery electrode and subsequent higher voltage. This is due to the asymmetric action of LICs, which serves as an enhancer of traditional supercapacitors. This culminates in the potential for pollution-free, long-lasting, and efficient energy-storing that is required to realise a renewable energy future. This review article offers an analysis of recent progress in the production of LIC electrode active materials, requirements and performance. In-situ hybridisation and ex-situ recombination of composite materials comprising a wide variety of active constituents is also addressed. The possible challenges and opportunities for future research based on LICs in energy applications are also discussed.
Non-Patent Literature
Authors: Velayos-Ortega Gema , López-Carreño Rosana
Published: February 2021

Non-patent literature is defined as scientific publications, technical standards, conference proceedings, clinical trials, books, manuals, technical or research reports, or any other technical scientific material which is cited in patents to show what has already been published and disseminated about the invention to be patented, in order to justify its novelty. These documents are considered technically relevant to the patent granting procedure and are cited along with other patents related to the same subject matter.
Sprouting of Sorghum (Sorghum bicolor [L.] Moench): Effect of Drying Treatment on Protein and Starch Features
Authors: Marchini Mia , Marti Alessandra , Folli Claudia , Prandi Barbara , Ganino Tommaso , Conte Paola , Fadda Costantino , Mattarozzi Monica , Carini Eleonora
Published: February 2021

The nutritional and physicochemical properties of sorghum proteins and starch make the use of this cereal for food production challenging. Sprouting is a cost-effective technology to improve the nutritional and functional profile of grains. Two drying treatments were used after sorghum sprouting to investigate whether the drying phase could improve the protein and starch functionalities. Results showed that the drying treatment at lower temperature/longer time (40 °C for 12 h) extended the enzymatic activity that started during sprouting compared to the one performed at higher temperature/shorter time (50 °C for 6 h). An increased protein hydrolysis and water- and oil-holding capacity were found in the flour obtained by the former treatment. Higher protein matrix hydrolysis caused high exposure of starch to enzymes, thus increasing its digestibility, while worsening the technological functionality. Overall, modulating drying conditions could represent a further way, in addition to sprouting, to improve sorghum flour’s nutritional profile.
World Heritage on the Move: Abandoning the Assessment of Authenticity to Meet the Challenges of the Twenty-First Century
Authors: Khalaf Roha W.
Published: February 2021

At a time when climate change, conflicts, disasters, and other global crises and challenges are increasingly affecting World Heritage properties, the utility of conservation assessment standards must be rethought. This article proposes abandoning the assessment of authenticity to treat properties less as “things”, deemed authentic or not, and more as evolving “processes” that embrace continuity and compatible change, which, it is argued, helps meet the challenges of the twenty-first century, namely climate change mitigation and adaptation; building back better after conflicts, disasters, or pandemics; and, ultimately, achieving sustainable development goals. Drawing on policy analysis and a wide range of literature, the article explains why authenticity is not a useful concept and why the idea of “heritage as process” is more relevant to the contemporary world. It shows how this idea can be put into effect and linked to Outstanding Universal Value, integrity, protection and management, which are already requirements in UNESCO’s Operational Guidelines for the implementation of the World Heritage Convention. In doing so, it contributes to aligning the implementation of the Convention with that of the global agendas of our time.
Influence of the COVID-19 Pandemic on Dental Emergency Admissions in an Urgent Dental Care Service in North Italy
Authors: Cagetti Maria Grazia , Balian Araxi , Camoni Nicole , Campus Guglielmo
Published: February 2021

A retrospective study was performed to verify if the number of admissions for urgent dental care in the Urgent Dental Care Service of San Paolo Hospital in Milan (Italy) was directly related to the different phases of the COVID-19 pandemic. Different periods were analyzed: 25 March–5 April 2019 (pre-COVID); 23 March–3 April 2020 (lockdown); 8 June–19 June 2020 (reopening); and November 9–November 20 (second wave). Raw data regarding admissions, diagnoses, and treatments were extracted. Descriptive and bivariate analyses were performed. The survey included 901 admissions, 285 in pre-COVID, 93 during lockdown, 353 in reopening, and 170 in the second wave. In each time period, statistically significant differences were found in the prevalence of each kind of diagnoses (χ2(3) = 20.33 p = 0.01 for endodontic emergencies, χ2(3) = 29.05 p < 0.01 for cellulitis/phlegmon, χ2(3) = 28.55 p < 0.01 for periodontal emergencies, Fisher’s Exact Test p < 0.01 for trauma, and χ2(3) = 59.94 p < 0.01 for all other kinds of diagnosis). A remarkable increase in consultations (+186.36%) and other treatments (+90.63%) occurred during reopening. Tooth extraction was the most frequently delivered treatment, but suffered the largest reduction during lockdown (−79.82%). The COVID-19 pandemic has highly affected dental activity in north Italy, underling the weaknesses of a private dental system in a pandemic scenario.
The Mediating Effects of Protective Behavioral Strategies on the Relationship between Addiction-Prone Personality Traits and Alcohol-Related Problems among Emerging Adults
Authors: Nogueira-Arjona Raquel , Thompson Kara , Milios Athena , Maloney Alyssa , Krupa Terry , Dobson Keith S. , Chen Shu-Ping , Stewart Sherry H.
Published: February 2021

Alcohol consumption and associated harms are an issue among emerging adults, and protective behavioral strategies (PBS) are actions with potential to minimize these harms. We conducted two studies aimed at determining whether the associations of at-risk personality traits (sensation-seeking [SS], impulsivity [IMP], hopelessness [HOP], and anxiety-sensitivity [AS]) with increased problematic alcohol use could be explained through these variables’ associations with decreased PBS use. We tested two mediation models in which the relationship between at-risk personality traits and increased problematic alcohol use outcomes (Study 1: Alcohol volume; Study 2: Heavy episodic drinking and alcohol-related harms) was partially mediated through decreased PBS use. Two samples of college students participated (N1 = 922, Mage1 = 20.11, 70.3% female; N2 = 1625, Mage2 = 18.78, 70.3% female). Results partially supported our hypotheses, providing new data on a mechanism that helps to explain the relationships between certain at-risk personality traits and problematic alcohol use, as these personalities are less likely to use PBS. In contrast, results showed that AS was positively related to alcohol-related harms and positively related to PBS, with the mediational path through PBS use being protective against problematic alcohol use. This pattern suggests that there are other factors/mediators working against the protective PBS pathway such that, overall, AS still presents risks for alcohol-related harms.
SERS-Based Colloidal Aptasensors for Quantitative Determination of Influenza Virus
Authors: Gribanyov Dmitry , Zhdanov Gleb , Olenin Andrei , Lisichkin Georgii , Gambaryan Alexandra , Kukushkin Vladimir , Zavyalova Elena
Published: February 2021

Development of sensitive techniques for rapid detection of viruses is on a high demand. Surface-enhanced Raman spectroscopy (SERS) is an appropriate tool for new techniques due to its high sensitivity. DNA aptamers are short structured oligonucleotides that can provide specificity for SERS biosensors. Existing SERS-based aptasensors for rapid virus detection had several disadvantages. Some of them lacked possibility of quantitative determination, while others had sophisticated and expensive implementation. In this paper, we provide a new approach that combines rapid specific detection and the possibility of quantitative determination of viruses using the example of influenza A virus.
Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation
Authors: Sedlář Antonín , Trávníčková Martina , Matějka Roman , Pražák Šimon , Mészáros Zuzana , Bojarová Pavla , Bačáková Lucie , Křen Vladimír , Slámová Kristýna
Published: February 2021

Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A165 on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A165 have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.
Synthesis, Characterization, and Encapsulation of Novel Plant Growth Regulators (PGRs) in Biopolymer Matrices
Authors: Vlahoviček-Kahlina Kristina , Jurić Slaven , Marijan Marijan , Mutaliyeva Botagoz , Khalus Svetlana V. , Prosyanik Alexander V. , Vinceković Marko
Published: February 2021

Novel plant growth regulators (PGRs) based on the derivatives of dehydroamino acids 2,3-dehydroaspartic acid dimethyl ester (PGR1), Z-isomer of the potassium salt of 2-amino-3-methoxycarbonylacrylic acid (PGR2) and 1-methyl-3-methylamino-maleimide (PGR3) have been synthesized and their growth-regulating properties investigated. Laboratory testing revealed their plant growth-regulating activity. PGR1 showing the most stimulating activity on all laboratory tested cultures were used in field experiments. Results showed that PGR1 is a highly effective environmentally friendly plant growth regulator with effects on different crops. Biopolymeric microcapsule formulations (chitosan/alginate microcapsule loaded with PGR) suitable for application in agriculture were prepared and characterized. Physicochemical properties and release profiles of PGRs from microcapsule formulations depend on the molecular interactions between microcapsule constituents including mainly electrostatic interactions and hydrogen bonds. The differences in the microcapsule formulations structure did not affect the mechanism of PGRs release which was identified as diffusion through microcapsules. The obtained results opened a perspective for the future use of microcapsule formulations as new promising agroformulations with a sustained and target release for plant growth regulation.
In Search of Molecular Markers for Cerebellar Neurons
Authors: Tam Wing Yip , Wang Xia , Cheng Andy S. K. , Cheung Kwok-Kuen
Published: February 2021

The cerebellum, the region of the brain primarily responsible for motor coordination and balance, also contributes to non-motor functions, such as cognition, speech, and language comprehension. Maldevelopment and dysfunction of the cerebellum lead to cerebellar ataxia and may even be associated with autism, depression, and cognitive deficits. Hence, normal development of the cerebellum and its neuronal circuitry is critical for the cerebellum to function properly. Although nine major types of cerebellar neurons have been identified in the cerebellar cortex to date, the exact functions of each type are not fully understood due to a lack of cell-specific markers in neurons that renders cell-specific labeling and functional study by genetic manipulation unfeasible. The availability of cell-specific markers is thus vital for understanding the role of each neuronal type in the cerebellum and for elucidating the interactions between cell types within both the developing and mature cerebellum. This review discusses various technical approaches and recent progress in the search for cell-specific markers for cerebellar neurons.
Evidence for 2-Methoxyestradiol-Mediated Inhibition of Receptor Tyrosine Kinase RON in the Management of Prostate Cancer
Authors: Batth Izhar Singh , Huang Shih-Bo , Villarreal Michelle , Gong Jingjing , Chakravarthy Divya , Keppler Brian , Jayamohan Sridharan , Osmulski Pawel , Xie Jianping , Rivas Paul , Bedolla Roble , Liss Michael A. , Yeh I-Tien , Reddick Robert , Miyamoto Hiroshi , Ghosh Rita , Kumar Addanki P.
Published: February 2021

2-Methoxyestradiol (2-ME2) possesses anti-tumorigenic activities in multiple tumor models with acceptable tolerability profile in humans. Incomplete understanding of the mechanism has hindered its development as an anti-tumorigenic compound. We have identified for the first-time macrophage stimulatory protein 1 receptor (MST1R) as a potential target of 2-ME2 in prostate cancer cells. Human tissue validation studies show that MST1R (a.k.a RON) protein levels are significantly elevated in prostate cancer tissues compared to adjacent normal/benign glands. Serum levels of macrophage stimulatory protein (MSP), a ligand for RON, is not only associated with the risk of disease recurrence, but also significantly elevated in samples from African American patients. 2-ME2 treatment inhibited mechanical properties such as adhesion and elasticity that are associated with epithelial mesenchymal transition by downregulating mRNA expression and protein levels of MST1R in prostate cancer cell lines. Intervention with 2-ME2 significantly reduced tumor burden in mice. Notably, global metabolomic profiling studies identified significantly higher circulating levels of bile acids in castrated animals that were decreased with 2-ME2 intervention. In summary, findings presented in this manuscript identified MSP as a potential marker for predicting biochemical recurrence and suggest repurposing 2-ME2 to target RON signaling may be a potential therapeutic modality for prostate cancer.
Neuromonitoring of the Recurrent Laryngeal Nerve Reduces the Rate of Bilateral Vocal Cord Dysfunction in Planned Bilateral Thyroid Procedures
Authors: Smaxwil Constantin , Aleker Miriam , Altmeier Julia , Naddaf Ali , Busch Mirjam , Wagner Joachim , Harsch Simone , Ploner Oswald , Zielke Andreas
Published: February 2021

Purpose: Bilateral vocal cord dysfunction (bVCD) is a rare but feared complication of thyroid surgery. This long term retrospective study determined the effect of intraoperative neuromonitoring (IONM) of the recurrent laryngeal nerve (RLN) during thyroid surgeries with regard to the rate of bVCD and evaluated the frequency as well as the outcome of staged operations. Methods: Retrospective analysis of prospectively documented data (2000–2019) of a tertiary referral centers’ database. IONM started in 2000 and, since 2010, discontinuation of surgery was encouraged in planned bilateral surgeries to prevent bVCD, if non-transient loss of signal (ntLOS) occurred on the first side. Datasets of the most recent 40-month-period were assessed in detail to determine the clinical outcome of unilateral ntLOS in planned bilateral thyroid procedures. Results: Of 22,573 patients, 65 had bVCD (0.288%). The rate of bVCD decreased from 0.44 prior to 2010 to 0.09% after 2010 (p < 0.001, Chi2). Case reviews of the most recent 40 months period identified ntLOS in 113/3115 patients (3.6%, 2.2% NAR), of which 40 ntLOS were recorded during a planned bilateral procedure (n = 952, 2.1% NAR). Of 21 ntLOS occurring on the first side of the bilateral procedure, 15 procedures were stopped, subtotal contralateral resections were performed, and thyroidectomy was continued in 3 patients respectively, with the use of continuous vagal IONM. Eighteen cases of VCD were documented postop, and all but one patient had a full recovery. Seven patients had staged resections after 1 to 18 months (median 4) after the first procedure. Conclusion: IONM facilitates reduced postoperative bVCD rates. IONM is, therefore, recommendable in planned bilateral procedures. The rate of non-complete bilateral surgery after intraoperative non-transient LOS was 2%.