bluuebunny/crossref_metadata_2025 huggingface dataset:
Family Member, Best Friend, Child or ‘Just’ a Pet, Owners’ Relationship Perceptions and Consequences for Their Cats
Authors: Bouma Esther M. C. , Reijgwart Marsha L. , Dijkstra Arie
Published: December 2021

Describing the relationship with one’s cat in human terms might reflect an underlying anthropomorphic view of the relationship which might be associated with an owner’s behavior towards their cat and the cat’s living environment. Owners self-categorized the relationship with their cat as either a ‘member of the family’, ‘as a child’, ‘best friend’, or ‘a pet animal’. The extent to which owner- and cat-related factors influence these four relationship descriptions are examined in survey data of approximately 1800 cat owners. Differences in outdoor access, care during absence of the owner, and access to the bedroom are examined between the four relationship perceptions. The owner’s age and household composition, ideas about their cat’s equality, support, and dependency, and whether their cat is a pedigree were significantly associated with relationship description and explained 46% of the variance. Owners who perceive their cat as a child or best friend see their cat as loyal, empathetic, equal to family, and dependent on them for love and care. Their cats are less often left in the care of others, are allowed more often in the bedroom and have less often (unrestricted) outdoor access. Moreover, cats perceived as children are more likely to live in a multi-cat household. Our results provide insight in the factors that are related to different (anthropomorphic) perceptions of the human–cat relationship and how perceptions relate to the living environment of cats.
Stress and Coping Strategies of Nurses Working with Patients Infected with and Not Infected with SARS-CoV-2 Virus
Authors: Puto Grażyna , Jurzec Maria , Leja-Szpak Anna , Bonior Joanna , Muszalik Marta , Gniadek Agnieszka
Published: December 2021

Introduction: Working during the COVID-19 pandemic is a particular challenge for nurses because, while performing their daily routines, they are exposed to physical and social consequences of the SARS-CoV-2 virus, which is accompanied by intensified stress. The aim of this study was to assess the intensity of stress and coping strategies applied by nurses working with both infected and non-infected patients with SARS-CoV-2 virus during the COVID-19 pandemic. Materials and Methods: The study was conducted between January and March 2021. Due to the epidemiological situation, the questionnaire was posted on Facebook in nurses’ groups and sent out via the “Messenger” and “WhatsApp” applications. Stress intensity was assessed by means of the Perceived Stress Scale (PSS-10), whereas coping strategies were assessed using the Mini-COPE stress coping inventory. Results: Among 151 surveyed nurses, more than half (52.3%) worked with infected patients and the remaining ones (47.7%) worked with non-infected patients. The level of stress perceived by nurses working with infected patients was higher than among nurses working with patients without SARS-CoV-2 infection (22.22 ± 5.94 vs. 20.21 ± 5.68, p = 0.03). The nurses working with infected patients were most likely to choose coping strategies focused on the problem (2.00 ± 0.62) and emotions (2.01 ± 0.69), whereas those working with non-infected patients usually chose strategies focused only on the problem (2.11 ± 0.58). Conclusions: During the COVID-19 pandemic, nurses working with SARS-CoV-2 patients experienced more intense stress than those working with non-infected patients. Nurses working with SARS-CoV-2 patients tended to cope with stress using strategies focused on the problem and on emotions, while those working with non-infected patients were more likely to choose strategies focused only on the problem.
Decoupling and Decomposition Analysis of Agricultural Carbon Emissions: Evidence from Heilongjiang Province, China
Authors: Huang Qinyi , Zhang Yu
Published: December 2021

Ensuring food security and curbing agricultural carbon emissions are both global policy goals. The evaluation of the relationship between grain production and agricultural carbon emissions is important for carbon emission reduction policymaking. This paper took Heilongjiang province, the largest grain-producing province in China, as a case study, estimated its grain production-induced carbon emissions, and examined the nexus between grain production and agricultural carbon emissions from 2000 to 2018, using decoupling and decomposition analyses. The results of decoupling analysis showed that weak decoupling occurred for half of the study period; however, the decoupling state and coupling state occurred alternately, and there was no definite evolving path from coupling to decoupling. Using the log mean Divisia index (LMDI) method, we decomposed the changes in agricultural carbon emissions into four factors: agricultural economy, agricultural carbon emission intensity, agricultural structure, and agricultural labor force effects. The results showed that the agricultural economic effect was the most significant driving factor for increasing agricultural carbon emissions, while the agricultural carbon emission intensity effect played a key inhibiting role. Further integrating decoupling analysis with decomposition analysis, we found that a low-carbon grain production mode began to take shape in Heilongjiang province after 2008, and the existing environmental policies had strong timeliness and weak persistence, probably due to the lack of long-term incentives for farmers. Finally, we suggested that formulating environmental policy should encourage farmers to adopt environmentally friendly production modes and technologies through taxation, subsidies, and other economic means to achieve low-carbon agricultural goals in China.
ESG Performance and Stock Price Volatility in Public Health Crisis: Evidence from COVID-19 Pandemic
Authors: Zhou Dongyi , Zhou Rui
Published: December 2021

Unlike traditional financial crises, COVID-19 is a global public health crisis with a significant negative impact on the global economy. Meanwhile, the stock market has been hit hard, and corporate share prices have become more volatile. However, the stock prices of some enterprises with good performance of ESG (Environment, Social, and Governance) are relatively stable in the epidemic. This paper selects ESG rating data from MSCI (Morgan Stanley Capital International) with better differentiation, adopts multiple regression and dummy variables, and adopts the Differences-in-Differences (DID)model with the help of COVID-19, an exogenous event. Empirical test the impact of ESG performance on the company’s stock price fluctuations. The results show that the stock price volatility of companies with good ESG performance is lower than that of companies with poor performance. Second, COVID-19 exacerbates volatility in company stock prices, but the increase in stock price volatility of companies with good ESG performance is small. That is, good ESG performance helps reduce the increase in stock price volatility due to COVID-19 shock, and plays a role in enhancing “resilience” and stabilizing stock prices. This paper provides new empirical evidence for the study of ESG performance and corporate stock price volatility, and puts forward relevant policy recommendations for enterprises and government departments.
Cascading Failure Analysis on Shanghai Metro Networks: An Improved Coupled Map Lattices Model Based on Graph Attention Networks
Authors: Ye Haonan , Luo Xiao
Published: December 2021

Analysis of the robustness and vulnerability of metro networks has great implications for public transport planning and emergency management, particularly considering passengers’ dynamic behaviors. This paper presents an improved coupled map lattices (CMLs) model based on graph attention networks (GAT) to study the cascading failure process of metro networks. The proposed model is applied to the Shanghai metro network using the automated fare collection (AFC) data, and the passengers’ dynamic behaviors are simulated by GAT. The quantitative cascading failure analysis shows that Shanghai metro network is robust to random attacks, but fragile to intentional attacks. Moreover, there is an approximately normal distribution between instant cascading failure speed and time step and the perturbation in a station which leads to steady state is approximately a constant. The result shows that a station surrounded by other densely distributed stations can trigger cascading failure faster and the cascading failure triggered by low-level accidents will spread in a short time and disappear quickly. This study provides an effective reference for dynamic safety evaluation and emergency management in metro networks.
Knowledge and Perceptions Regarding Coronavirus (COVID-19) among Pediatric Dentists during Lockdown Period
Authors: Mallineni Sreekanth , Nuvvula Sivakumar , Bhumireddy Jaya , Ismail Ahmad , Verma Priya , Sajja Rishitha , Alassaf Abdullah , Almulhim Basim , Alghamdi Sara , Saha Anupam , Goyal Virinder , Namineni Srinivas
Published: December 2021

Aim: To assess the knowledge and perceptions of COVID-19 among pediatric dentists based on their dependent source of information. Methods: A descriptive-analytical cross-sectional survey using a self-administered questionnaire with 23 questions was sent via Google forms to pediatric dentists. All participants were divided into three groups [postgraduate residents (PGs), private practitioners (PP), and faculty (F)]. The comparison of knowledge and perception scores was made based on occupation, source of information, and descriptive statistics used for the analysis using SPSS 21.0 (IBM, Armonk, NY, USA). Results: A total of 291 pediatric dentists completed the survey, and the majority of them were females (65%). Overall, good mean scores were obtained for knowledge (9.2 ± 1.07) and perceptions (5.6 ± 1.5). The majority of the participants used health authorities (45%) to obtain updates on COVID-19, while social media (35.1%) and both (19.6%) accounted for the next two. A statistically significant difference (p < 0.05) was found among different pediatric dentists groups for relying on the source of information. Conclusion: Overall good pediatric dentists showed sufficient knowledge regarding COVID-19. The pediatric dentists’ age, occupation, and source of information influenced knowledge regarding COVID-19, whereas perceptions were influenced by age and gender of the participants. Health authorities successfully educated pediatric dentists than the social media
Ischemic Heart Disease in Nigeria: Exploring the Challenges, Current Status, and Impact of Lifestyle Interventions on Its Primary Healthcare System
Authors: Nnate Daniel A. , Eleazu Chinedum O. , Abaraogu Ukachukwu O.
Published: December 2021

The burden of ischemic heart disease in Nigeria calls for an evidence-based, innovative, and interdisciplinary approach towards decreasing health inequalities resulting from individual lifestyle and poor socioeconomic status in order to uphold the holistic health of individuals to achieve global sustainability and health equity. The poor diagnosis and management of ischemic heart disease in Nigeria contributes to the inadequate knowledge of its prognosis among individuals, which often results in a decreased ability to seek help and self-care. Hence, current policies aimed at altering lifestyle behaviour to minimize exposure to cardiovascular risk factors may be less suitable for Nigeria’s diverse culture. Mitigating the burden of ischemic heart disease through the equitable access to health services and respect for the autonomy and beliefs of individuals in view of achieving Universal Health Coverage (UHC) requires comprehensive measures to accommodate, as much as possible, every individual, notwithstanding their values and socioeconomic status.
A Conceptual Framework Proposal for a Noise Modelling Service for Drones in U-Space Architecture
Authors: Langen Tommy , Nunavath Vimala , Dahle Ole Henrik
Published: December 2021

In recent years, there has been a rapid growth in the development and usage of flying drones due to their diverse capabilities worldwide. Public and private sectors will actively use drone technology in the logistics of goods and transporting passengers in the future. There are concerns regarding privacy and noise exposure in and around the rural and urban environment with the rapid expansion. Further, drone noise could affect human health. European Union has defined a service-orientated architecture to provide air traffic management for drones, called U-space. However, it lacks a noise modelling service (NMS). This paper proposes a conceptual framework for such a noise modelling service for drones with a use case scenario and verification method. The framework is conceptualized based on noise modelling from the aviation sector. The NMS can be used to model the noise to understand the accepted drone noise levels in different scenarios and take measures needed to reduce the noise impact on the community.
Spirituality in Patients at the End of Life—Is It Necessary? A Qualitative Approach to the Protagonists
Authors: García-Navarro E. Begoña , Medina-Ortega Alicia , García Navarro Sonia
Published: December 2021

Spirituality is the most unknown aspect of palliative care despite being the need that is most altered in the last moments of life. Objective. To identify on the one hand the spiritual needs of patients who are at the end of life and on the other hand, the way in which nursing professionals can work to provide effective accompaniment in this process. Method. A qualitative study was conducted which applied different data collection techniques. This was done to describe the phenomenon from a holistic perspective in relation to experts’ perceptions of the competencies required by health professionals and palliative patients’ spiritual needs. Semi-structured interviews were conducted within both populations. In order to analyze the qualitative data collected through interviews, discourse was analyzed according to the Taylor–Bodgan model and processed using Atlas.ti software. Results. Three well-differentiated lines of argument are extracted from the discourse in each of the groups, on the one hand in the group of patients they define the concept of spirituality, system of values and beliefs, and the Factors that influence the spirituality of patients at the end of life (differentiating palliative care areas/other areas) and on the other, the professionals agree with the patients in the line of argument of concept of spirituality although they define more metaphysical categories and the other two lines of argument that result are the spiritual attention in this process and the need for formation in spirituality. Conclusions. The provision of spiritual care gives meaning to the actions of nursing professionals when it comes to providing end-of-life care, achieving holistic care, humanizing death, and promoting a dignified end.
Impact of Shyness on Self-Esteem: The Mediating Effect of Self-Presentation
Authors: Bober Adrianna , Gajewska Ewa , Czaprowska Anna , Świątek Agata Hiacynta , Szcześniak Małgorzata
Published: December 2021

Background: Although the relationship between shyness and self-esteem is well described in the psychological literature, far less is known about the potential mechanisms that underlie this association. The main goal of the current work is to verify whether self-presentation acts as a mediating variable between both constructs. Methods: The study was carried out among 198 adults. The Revised Cheek and Buss Shyness Scale, the Rosenberg Self-Esteem Scale, and the Self-Presentation Style Questionnaire were applied. Results: A large and positive correlation coefficient was observed between the following variables: (1) self-esteem/self-promotion; (2) shyness/self-deprecation. All other variables correlated negatively: (1) shyness/self-esteem; (2) shyness/self-promotion; (3) self-esteem/self-deprecation; (4) self-promotion/self-deprecation. Moreover, both self-promotion and self-deprecation acted as mediators between life satisfaction and self-esteem. Conclusion: The outcomes of the present study show a new mediating aspect for the direct relationship between shyness and self-esteem in the form of two styles of self-presentation. The results indicate that the tendency of shy people to avoid others can have a lower effect on their overall sense of self-esteem when they try to present themselves in a clearly favorable light. By contrast, shyness may have a stronger impact on their sense of self-worth when they present themselves as helpless, unsure, and incompetent.
Release of Heavy Metals from Plastic Statuettes Used as Souvenirs and/or Toys Handled by Children
Authors: Pandolfi Pietro , Sammartino Maria Pia , Visco Giovanni , Avino Pasquale , Stillittano Virgilio
Published: December 2021

Different plastic toys are on sale in the Italian market: they are sold as souvenirs and/or as toys. Such statuettes, called Gongoli, represent a famous character (a soccer player, a politician, the Pope, etc.). In particular, these products are widely sold, but the material composition is not sufficiently defined. Further, the effect of the release of dangerous compounds on human health is not sufficiently documented. Following this hypothesis, a study on eight different statuettes was carried out both for evaluating the possible presence of heavy metals and for evidencing their release from these objects. Preliminary analysis by means of EDS spectroscopy highlighted the percentage chemical composition of different products, especially the presence of total Cr and Ni. Release tests evidenced the release of Cr, Cu, Ni, and Pb: Pb reached 74 mg kg−1, which is an interesting value even if it is lower than reported in the legislation. This study should be considered preliminary due to its limitations, such as the number of items investigated and the large variability found for some elements, but it highlights a serious problem related to the classification of these products which are marketed as souvenirs but manipulated by children.
Implementation and Outcomes of a Comprehensive Tobacco Free Workplace Program in Opioid Treatment Centers
Authors: Taing Matthew , Nitturi Vijay , Chen Tzuan A. , Kyburz Bryce , Martinez Leal Isabel , Correa-Fernández Virmarie , Obasi Ezemenari M. , Williams Teresa , Casey Kathleen , O’Connor Daniel P. , Koshy Litty , Britton Maggie , Drenner Kelli , Reitzel Lorraine R.
Published: December 2021

Tobacco use is exceedingly high among individuals receiving care for opioid addiction, but not commonly addressed by clinicians in treatment settings. Taking Texas Tobacco Free (TTTF) is a comprehensive tobacco-free workplace (TFW) program that builds treatment centers’ capacity to address tobacco use with evidence-based tobacco cessation policies and practices. Here, we examine the process and outcomes of TTTF’s implementation within 7 opioid addiction centers. Program goals were structured according to the RE-AIM framework. Pre- and post-implementation data were collected from client facing and non-client facing employees to assess changes in education, training receipt, knowledge, and intervention behaviors, relative to program goals. Centers reported tobacco screenings conducted and nicotine replacement therapy (NRT) delivered through 6 months post-implementation. Overall, 64.56% of employees participated in TTTF-delivered tobacco education, with a 54.9% gain in tobacco control and treatment knowledge (p < 0.0001), and significant increases in exposure to education about tobacco use and harms among individuals with opioid use disorder (p = 0.0401). There were significant gains in clinicians’ receipt of training in 9/9 tobacco education areas (ps ≤ 0.0118). From pre- to post-implementation, there were mean increases in the use of the 5A’s (ask, advise, assess, assist, and arrange) and other evidence-based interventions for tobacco cessation, with statistically significant gains seen in NRT provision/referral (p < 0.0001). Several program goals were achieved or exceeded; however, 100% center participation in specialized clinical trainings was among notable exceptions. One program withdrew due to competing pandemic concerns; all others implemented comprehensive TFW policies. Overall, TTTF may have improved participating opioid treatment centers’ capacity to address tobacco use, although study limitations, including lower post-implementation evaluation response rates, suggest that results require replication in other opioid addiction treatment settings.
Does the COVID-19 Vaccine Still Work That “Most of the Confirmed Cases Had Been Vaccinated”? A Content Analysis of Vaccine Effectiveness Discussion on Sina Weibo during the Outbreak of COVID-19 in Nanjing
Authors: Gao Hao , Zhao Qingting , Ning Chuanlin , Guo Difan , Wu Jing , Li Lina
Published: December 2021

In July 2021, breakthrough cases were reported in the outbreak of COVID-19 in Nanjing, sparking concern and discussion about the vaccine’s effectiveness and becoming a trending topic on Sina Weibo. In order to explore public attitudes towards the COVID-19 vaccine and their emotional orientations, we collected 1542 posts under the trending topic through data mining. We set up four categories of attitudes towards COVID-19 vaccines, and used a big data analysis tool to code and manually checked the coding results to complete the content analysis. The results showed that 45.14% of the Weibo posts (n = 1542) supported the COVID-19 vaccine, 12.97% were neutral, and 7.26% were doubtful, which indicated that the public did not question the vaccine’s effectiveness due to the breakthrough cases in Nanjing. There were 66.47% posts that reflected significant negative emotions. Among these, 50.44% of posts with negative emotions were directed towards the media, 25.07% towards the posting users, and 11.51% towards the public, which indicated that the negative emotions were not directed towards the COVID-19 vaccine. External sources outside the vaccine might cause vaccine hesitancy. Public opinions expressed in online media reflect the public’s cognition and attitude towards vaccines and their core needs in terms of information. Therefore, online public opinion monitoring could be an essential way to understand the opinions and attitudes towards public health issues.
Occurrence of Antibiotic-Resistant Staphylococcus spp. in Orange Orchards in Thailand
Authors: Rattanapunya Siwalee , Deethae Aomhatai , Woskie Susan , Kongthip Pornpimol , Matthews Karl R.
Published: December 2021

Background: The widespread indiscriminate application of antibiotics to food crops to control plant disease represents a potential human health risk. In this study, the presence of antibiotic-resistant staphylococci associated with workers and orange orchard environments was determined. A total of 20 orchards (orange and other fruits) were enrolled in the study. Trees in the orange orchards were treated with ampicillin on a pre-determined schedule. Environmental samples (n = 60) included soil, water, and oranges; 152 hand and nasal samples were collected from 76 healthy workers. Antibiotic susceptibility profiles were determined for all staphylococcal isolates. Results: This investigation revealed that of the total Staphylococcus spp. recovered from the orange orchard, 30% (3/10) were resistant to erythromycin, 20% (2/10) were resistant to ampicillin, and 20% (2/10) resistant to both erythromycin and ampicillin. Conclusion: The application of antibiotics to orange trees in open production environments to halt the spread of bacterial disease presents risks to the environment and creates health concerns for Thai farmers using those agents. ARB on crops such as oranges may enter the global food supply and adversely affect public health.
Analyses and Correlation of Pathologic and Ocular Cutaneous Changes in Murine Graft versus Host Disease
Authors: Levy Robert B. , Mousa Hazem M. , Lightbourn Casey O. , Shiuey Eric J. , Latoni David , Duffort Stephanie , Flynn Ryan , Du Jing , Barreras Henry , Zaiken Michael , Paz Katelyn , Blazar Bruce R. , Perez Victor L.
Published: December 2021

Graft versus host disease (GVHD) is initiated by donor allo-reactive T cells activated against recipient antigens. Chronic GVHD (cGVHD) is characterized by immune responses that may resemble autoimmune features present in the scleroderma and Sjogren’s syndrome. Unfortunately, ocular involvement occurs in approximately 60–90% of patients with cGVHD following allo-hematopoietic stem cell transplants (aHSCT). Ocular GVHD (oGVHD) may affect vision due to ocular adnexa damage leading to dry eye and keratopathy. Several other compartments including the skin are major targets of GVHD effector pathways. Using mouse aHSCT models, the objective was to characterize cGVHD associated alterations in the eye and skin to assess for correlations between these two organs. The examination of multiple models of MHC-matched and MHC-mismatched aHSCT identified a correlation between ocular and cutaneous involvement accompanying cGVHD. Studies detected a “positive” correlation, i.e., when cGVHD-induced ocular alterations were observed, cutaneous compartment alterations were also observed. When no or minimal ocular signs were detected, no or minimal skin changes were observed. In total, these findings suggest underlying cGVHD-inducing pathological immune mechanisms may be shared between the eye and skin. Based on the present observations, we posit that when skin involvement is present in aHSCT patients with cGVHD, the evaluation of the ocular surface by an ophthalmologist could potentially be of value.
GUN4 Affects the Circadian Clock and Seedlings Adaptation to Changing Light Conditions
Authors: Li Tao , Wu Rui , Liu Zhixin , Wang Jiajing , Guo Chenxi , Zhou Yaping , Bawa George , Sun Xuwu
Published: December 2021

The chloroplast is a key organelle for photosynthesis and perceiving environmental information. GENOME UNCOUPLED 4 (GUN4) has been shown to be required for the regulation of both chlorophyll synthesis, reactive oxygen species (ROS) homeostasis and plastid retrograde signaling. In this study, we found that growth of the gun4 mutant was significantly improved under medium strong light (200 μmol photons m−2s−1) compared to normal light (100 μmol photons m−2s−1), in marked contrast to wild-type (WT). Further analysis revealed that GUN4 interacts with SIGNAL RECOGNITION PARTICLE 54 KDA SUBUNIT (SRP43) and SRP54. RNA-seq analysis indicated that the expression of genes for light signaling and the circadian clock is altered in gun4 compared with (WT). qPCR analysis confirmed that the expression of the clock genes CLOCK-RELATED 1 (CCA1), LATE ELONGATION HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION 1 (TOC1) and PSEUDO RESPONSE REGULATOR 7 (PRR7) is significantly changed in the gun4 and srp54 mutants under normal and medium strong light conditions. These results suggest that GUN4 may coordinate the adaptation of plants to changing light conditions by regulating the biological clock, although it is not clear whether the effect is direct or indirect.
The Effects of Separate and Combined Treatment of Male Rats with Type 2 Diabetes with Metformin and Orthosteric and Allosteric Agonists of Luteinizing Hormone Receptor on Steroidogenesis and Spermatogenesis
Authors: Bakhtyukov Andrey A. , Derkach Kira V. , Sorokoumov Viktor N. , Stepochkina Anna M. , Romanova Irina V. , Morina Irina Yu. , Zakharova Irina O. , Bayunova Liubov V. , Shpakov Alexander O.
Published: December 2021

In men with type 2 diabetes mellitus (T2DM), steroidogenesis and spermatogenesis are impaired. Metformin and the agonists of luteinizing hormone/human chorionic gonadotropin(hCG)-receptor (LH/hCG-R) (hCG, low-molecular-weight allosteric LH/hCG-R-agonists) can be used to restore them. The aim was to study effectiveness of separate and combined administration of metformin, hCG and 5-amino-N-tert-butyl-2-(methylsulfanyl)-4-(3-(nicotinamido)phenyl)thieno[2,3-d]pyrimidine-6-carboxamide (TP3) on steroidogenesis and spermatogenesis in male rats with T2DM. hCG (15 IU/rat/day) and TP3 (15 mg/kg/day) were injected in the last five days of five-week metformin treatment (120 mg/kg/day). Metformin improved testicular steroidogenesis and spermatogenesis and restored LH/hCG-R-expression. Compared to control, in T2DM, hCG stimulated steroidogenesis and StAR-gene expression less effectively and, after five-day administration, reduced LH/hCG-R-expression, while TP3 effects changed weaker. In co-administration of metformin and LH/hCG-R-agonists, on the first day, stimulating effects of LH/hCG-R-agonists on testosterone levels and hCG-stimulated expression of StAR- and CYP17A1-genes were increased, but on the 3–5th day, they disappeared. This was due to reduced LH/hCG-R-gene expression and increased aromatase-catalyzed estradiol production. With co-administration, LH/hCG-R-agonists did not contribute to improving spermatogenesis, induced by metformin. Thus, in T2DM, metformin and LH/hCG-R-agonists restore steroidogenesis and spermatogenesis, with metformin being more effective in restoring spermatogenesis, and their co-administration improves LH/hCG-R-agonist-stimulating testicular steroidogenesis in acute but not chronic administration.
Regulation and Function of Laminin A5 during Mouse and Human Decidualization
Authors: Yang Zhen-Shan , Pan Hai-Yang , Shi Wen-Wen , Chen Si-Ting , Wang Ying , Li Meng-Yuan , Zhang Hai-Yi , Yang Chen , Liu Ai-Xia , Yang Zeng-Ming
Published: December 2021

Decidualization is essential to the establishment of pregnancy in rodents and primates. Laminin A5 (encoding by Laminin α5) is a member of the laminin family, which is mainly expressed in the basement membranes. Although laminins regulate cellular phenotype maintenance, adhesion, migration, growth, and differentiation, the expression, function, and regulation of laminin A5 during early pregnancy are still unknown. Therefore, we investigated the expression and role of laminin A5 during mouse and human decidualization. Laminin A5 is highly expressed in mouse decidua and artificially induced deciduoma. Laminin A5 is significantly increased under in vitro decidualization. Laminin A5 knockdown significantly inhibits the expression of Prl8a2, a marker for mouse decidualization. Progesterone stimulates the expression of laminin A5 in ovariectomized mouse uterus and cultured mouse stromal cells. We also show that progesterone regulates laminin A5 through the PKA-CREB-C/EBPβ pathway. Laminin A5 is also highly expressed in human pregnant decidua and cultured human endometrial stromal cells during in vitro decidualization. Laminin A5 knockdown by siRNA inhibits human in vitro decidualization. Collectively, our study reveals that laminin A5 may play a pivotal role during mouse and human decidualization via the PKA-CREB-C/EBPβ pathway.
The AEROPILs Generation: Novel Poly(Ionic Liquid)-Based Aerogels for CO2 Capture
Authors: Barrulas Raquel V. , López-Iglesias Clara , Zanatta Marcileia , Casimiro Teresa , Mármol Gonzalo , Carrott Manuela Ribeiro , García-González Carlos A. , Corvo Marta C.
Published: December 2021

CO2 levels in the atmosphere are increasing exponentially. The current climate change effects motivate an urgent need for new and sustainable materials to capture CO2. Porous materials are particularly interesting for processes that take place near atmospheric pressure. However, materials design should not only consider the morphology, but also the chemical identity of the CO2 sorbent to enhance the affinity towards CO2. Poly(ionic liquid)s (PILs) can enhance CO2 sorption capacity, but tailoring the porosity is still a challenge. Aerogel’s properties grant production strategies that ensure a porosity control. In this work, we joined both worlds, PILs and aerogels, to produce a sustainable CO2 sorbent. PIL-chitosan aerogels (AEROPILs) in the form of beads were successfully obtained with high porosity (94.6–97.0%) and surface areas (270–744 m2/g). AEROPILs were applied for the first time as CO2 sorbents. The combination of PILs with chitosan aerogels generally increased the CO2 sorption capability of these materials, being the maximum CO2 capture capacity obtained (0.70 mmol g−1, at 25 °C and 1 bar) for the CHT:P[DADMA]Cl30%AEROPIL.
Dietary Supplementation with Vitamin D, Fish Oil or Resveratrol Modulates the Gut Microbiome in Inflammatory Bowel Disease
Authors: Wellington Vivian Naa Amua , Sundaram Vijaya Lakshmi , Singh Soudamani , Sundaram Uma
Published: December 2021

Gastrointestinal health is influenced by the functional genes and metabolites generated by the human microbiome. As the volume of current biomedical and translational research indicates, the importance and impact of this ecosystem of microorganisms, especially those comprising the gut microbiome on human health, has become increasingly apparent. Changes to the gut microbiome are associated with inflammatory bowel disease (IBD), which is characterized by persistent intestinal inflammation. Furthermore, the lifetime dietary choices of their host may positively or negatively affect both the gut microbiome and its impact on IBD. As such, “anti-inflammatory” dietary supplements, their impact, and mechanisms in restoring gut microbiota homeostasis during IBD is an area of intensive research. Dietary supplementation may represent an important adjuvant treatment avenue for limiting intestinal inflammation in IBD. Overall, this review addresses the development of the gut microbiome, the significance of the gut microbiome in IBD, and the use of dietary supplements such as vitamin D, fish oil, and resveratrol in the mitigation of IBD-associated gut dysbiosis and intestinal inflammation.
Mitochondrial Retinopathies
Authors: Zeviani Massimo , Carelli Valerio
Published: December 2021

The retina is an exquisite target for defects of oxidative phosphorylation (OXPHOS) associated with mitochondrial impairment. Retinal involvement occurs in two ways, retinal dystrophy (retinitis pigmentosa) and subacute or chronic optic atrophy, which are the most common clinical entities. Both can present as isolated or virtually exclusive conditions, or as part of more complex, frequently multisystem syndromes. In most cases, mutations of mtDNA have been found in association with mitochondrial retinopathy. The main genetic abnormalities of mtDNA include mutations associated with neurogenic muscle weakness, ataxia and retinitis pigmentosa (NARP) sometimes with earlier onset and increased severity (maternally inherited Leigh syndrome, MILS), single large-scale deletions determining Kearns–Sayre syndrome (KSS, of which retinal dystrophy is a cardinal symptom), and mutations, particularly in mtDNA-encoded ND genes, associated with Leber hereditary optic neuropathy (LHON). However, mutations in nuclear genes can also cause mitochondrial retinopathy, including autosomal recessive phenocopies of LHON, and slowly progressive optic atrophy caused by dominant or, more rarely, recessive, mutations in the fusion/mitochondrial shaping protein OPA1, encoded by a nuclear gene on chromosome 3q29.
Analysis of the Genome of the Heavy Metal Resistant and Hydrocarbon-Degrading Rhizospheric Pseudomonas qingdaonensis ZCR6 Strain and Assessment of Its Plant-Growth-Promoting Traits
Authors: Chlebek Daria , Płociniczak Tomasz , Gobetti Sara , Kumor Agata , Hupert-Kocurek Katarzyna , Pacwa-Płociniczak Magdalena
Published: December 2021

The Pseudomonas qingdaonensis ZCR6 strain, isolated from the rhizosphere of Zea mays growing in soil co-contaminated with hydrocarbons and heavy metals, was investigated for its plant growth promotion, hydrocarbon degradation, and heavy metal resistance. In vitro bioassays confirmed all of the abovementioned properties. ZCR6 was able to produce indole acetic acid (IAA), siderophores, and ammonia, solubilized Ca3(PO4)2, and showed surface active properties and activity of cellulase and very high activity of 1-aminocyclopropane-1-carboxylic acid deaminase (297 nmol α-ketobutyrate mg−1 h−1). The strain degraded petroleum hydrocarbons (76.52% of the initial hydrocarbon content was degraded) and was resistant to Cd, Zn, and Cu (minimal inhibitory concentrations reached 5, 15, and 10 mM metal, respectively). The genome of the ZCR6 strain consisted of 5,507,067 bp, and a total of 5055 genes were annotated, of which 4943 were protein-coding sequences. Annotation revealed the presence of genes associated with nitrogen fixation, phosphate solubilization, sulfur metabolism, siderophore biosynthesis and uptake, synthesis of IAA, ethylene modulation, heavy metal resistance, exopolysaccharide biosynthesis, and organic compound degradation. Complete characteristics of the ZCR6 strain showed its potential multiway properties for enhancing the phytoremediation of co-contaminated soils. To our knowledge, this is the first analysis of the biotechnological potential of the species P. qingdaonensis.
Discrimination of Transgenic Canola (Brassica napus L.) and their Hybrids with B. rapa using Vis-NIR Spectroscopy and Machine Learning Methods
Authors: Sohn Soo-In , Pandian Subramani , Zaukuu John-Lewis Zinia , Oh Young-Ju , Park Soo-Yun , Na Chae-Sun , Shin Eun-Kyoung , Kang Hyeon-Jung , Ryu Tae-Hun , Cho Woo-Suk , Cho Youn-Sung
Published: December 2021

In recent years, the rapid development of genetically modified (GM) technology has raised concerns about the safety of GM crops and foods for human health and the ecological environment. Gene flow from GM crops to other crops, especially in the Brassicaceae family, might pose a threat to the environment due to their weediness. Hence, finding reliable, quick, and low-cost methods to detect and monitor the presence of GM crops and crop products is important. In this study, we used visible near-infrared (Vis-NIR) spectroscopy for the effective discrimination of GM and non-GM Brassica napus, B. rapa, and F1 hybrids (B. rapa X GM B. napus). Initially, Vis-NIR spectra were collected from the plants, and the spectra were preprocessed. A combination of different preprocessing methods (four methods) and various modeling approaches (eight methods) was used for effective discrimination. Among the different combinations, the Savitzky-Golay and Support Vector Machine combination was found to be an optimal model in the discrimination of GM, non-GM, and hybrid plants with the highest accuracy rate (100%). The use of a Convolutional Neural Network with Normalization resulted in 98.9%. The same higher accuracy was found in the use of Gradient Boosted Trees and Fast Large Margin approaches. Later, phenolic acid concentration among the different plants was assessed using GC-MS analysis. Partial least squares regression analysis of Vis-NIR spectra and biochemical characteristics showed significant correlations in their respective changes. The results showed that handheld Vis-NIR spectroscopy combined with chemometric analyses could be used for the effective discrimination of GM and non-GM B. napus, B. rapa, and F1 hybrids. Biochemical composition analysis can also be combined with the Vis-NIR spectra for efficient discrimination.
Endothelial ADAM17 Expression in the Progression of Kidney Injury in an Obese Mouse Model of Pre-Diabetes
Authors: Palau Vanesa , Jarrín Josué , Villanueva Sofia , Benito David , Márquez Eva , Rodríguez Eva , Soler María José , Oliveras Anna , Gimeno Javier , Sans Laia , Crespo Marta , Pascual Julio , Barrios Clara , Riera Marta
Published: December 2021

Disintegrin and metalloproteinase domain 17 (ADAM17) activates inflammatory and fibrotic processes through the shedding of various molecules such as Tumor Necrosis Factor-α (TNF-α) or Transforming Growht Factor-α (TGF-α). There is a well-recognised link between TNF-α, obesity, inflammation, and diabetes. In physiological situations, ADAM17 is expressed mainly in the distal tubular cell while, in renal damage, its expression increases throughout the kidney including the endothelium. The aim of this study was to characterize, for the first time, an experimental mouse model fed a high-fat diet (HFD) with a specific deletion of Adam17 in endothelial cells and to analyse the effects on different renal structures. Endothelial Adam17 knockout male mice and their controls were fed a high-fat diet, to induce obesity, or standard rodent chow, for 22 weeks. Glucose tolerance, urinary albumin-to-creatinine ratio, renal histology, macrophage infiltration, and galectin-3 levels were evaluated. Results showed that obese mice presented higher blood glucose levels, dysregulated glucose homeostasis, and higher body weight compared to control mice. In addition, obese wild-type mice presented an increased albumin-to-creatinine ratio; greater glomerular size and mesangial matrix expansion; and tubular fibrosis with increased galectin-3 expression. Adam17 deletion decreased the albumin-to-creatinine ratio, glomerular mesangial index, and tubular galectin-3 expression. Moreover, macrophage infiltration in the glomeruli of obese Adam17 knockout mice was reduced as compared to obese wild-type mice. In conclusion, the expression of ADAM17 in endothelial cells impacted renal inflammation, modulating the renal function and histology in an obese pre-diabetic mouse model.
Linoleic Acid Upregulates Microrna-494 to Induce Quiescence in Colorectal Cancer
Authors: Ogata Ruiko , Mori Shiori , Kishi Shingo , Sasaki Rika , Iwata Naoya , Ohmori Hitoshi , Sasaki Takamitsu , Nishiguchi Yukiko , Nakashima Chie , Goto Kei , Kawahara Isao , Fujiwara-Tani Rina , Kuniyasu Hiroki
Published: December 2021

Cancer dormancy is a state characterized by the quiescence of disseminated cancer cells, and tumor recurrence occurs when such cells re-proliferate after a long incubation period. These cancer cells tend to be treatment resistant and one of the barriers to successful therapeutic intervention. We have previously reported that long-term treatment of cancer cells with linoleic acid (LA) induces a dormancy-like phenotype. However, the mechanism underpinning this effect has not yet been clarified. Here, we investigate the mechanism of LA-induced quiescence in cancer cells. We first confirmed that long-term treatment of the mouse colorectal cancer cell line CT26 with LA induced quiescence. When these cells were inoculated subcutaneously into a syngeneic mouse and fed with an LA diet, the inoculated cancer cells maintained the quiescent state and exhibited markers of dormancy. LA-treated CT26 cells showed reduced oxidative phosphorylation, glycolysis, and energy production as well as reduced expression of the regulatory factors Pgc1α and MycC. MicroRNA expression profiling revealed that LA induced an upregulation in miR-494. The expression of Pgc1α and MycC were both induced by an miR-494 mimic, and the LA-induced decrease in gene expression was abrogated by an miR-494 inhibitor. The expression of miR-494 was enhanced by the mitochondrial oxidative stress produced by LA. In a syngeneic mouse subcutaneous tumor model, growth suppression by an LA diet and growth delay by LA pretreatment + LA diet were found to have similar effects as administration of an miR-494 mimic. In contrast, the effects of LA were abrogated by an miR-494 inhibitor. Analysis of human colorectal cancer tissue revealed that miR-494 was present at low levels in non-metastatic cases and cases with simultaneous liver metastases but was expressed at high levels in cases with delayed liver metastases, which also exhibited reduced expression of PGC1α and MYCC. These results suggest that miR-494 is involved in cancer dormancy induced by high levels of LA intake and that this microRNA may be valuable in targeting dormant cancer cells.
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
Authors: Oh Jin-Su , Seong Geum-Su , Kim Yong-Deok , Choung Se-Young
Published: December 2021

The prevalence of atopic dermatitis (AD), a disease characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, is rapidly increasing worldwide. Deacetylasperulosidic acid (DAA) has anti-atopic activity in the three main cell types associated with AD: keratinocytes, mast cells, and eosinophils. Our study investigated the anti-atopic activity of DAA in 2,4-dinitrochlorobenzene-induced NC/Nga mice. DAA alleviated the symptoms of AD, including infiltration of inflammatory cells (mast cells and eosinophils), epidermal thickness, ear thickness, and scratching behavior. Furthermore, DAA reduced serum IgE, histamine, and IgG1/IgG2a ratio and modulated the levels of AD-related cytokines and chemokines, namely interleukin (IL)-1β, IL-4, IL-6, IL-9, IL-10, IL-12, tumor necrosis factor-α, interferon-γ, thymic stromal lymphopoietin, thymus and activation-regulated chemokine, macrophage-derived chemokine, and regulated on activation the normal T cell expressed and secreted in the serum. DAA restored immune balance by regulating gene expression and secretion of Th1-, Th2-, Th9-, Th17-, and Th22-mediated inflammatory factors in the dorsal skin and splenocytes and restored skin barrier function by increasing the expression of the pro-filaggrin gene and barrier-related proteins filaggrin, involucrin, and loricrin. These results suggest DAA as a potential therapeutic agent that can alleviate the symptoms of AD by reducing pruritus, modulating immune imbalance, and restoring skin barrier function.
Morphometry and Stiffness of Red Blood Cells—Signatures of Neurodegenerative Diseases and Aging
Authors: Strijkova-Kenderova Velichka , Todinova Svetla , Andreeva Tonya , Bogdanova Desislava , Langari Ariana , Danailova Avgustina , Krumova Sashka , Zlatareva Elena , Kalaydzhiev Nikolay , Milanov Ivan , Taneva Stefka G.
Published: December 2021

Human red blood cells (RBCs) are unique cells with the remarkable ability to deform, which is crucial for their oxygen transport function, and which can be significantly altered under pathophysiological conditions. Here we performed ultrastructural analysis of RBCs as a peripheral cell model, looking for specific signatures of the neurodegenerative pathologies (NDDs)—Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS) and Alzheimer’s disease (AD), utilizing atomic force (AFM) and conventional optical (OM) microscopy. We found significant differences in the morphology and stiffness of RBCs isolated from patients with the selected NDDs and those from healthy individuals. Neurodegenerative pathologies’ RBCs are characterized by a reduced abundance of biconcave discoid shape, lower surface roughness and a higher Young’s modulus, compared to healthy cells. Although reduced, the biconcave is still the predominant shape in ALS and AD cells, while the morphology of PD is dominated by crenate cells. The features of RBCs underwent a marked aging-induced transformation, which followed different aging pathways for NDDs and normal healthy states. It was found that the diameter, height and volume of the different cell shape types have different values for NDDs and healthy cells. Common and specific morphological signatures of the NDDs were identified.