bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimizing Energy Efficiency with a Cloud-Based Model Predictive Control: A Case Study of a Multi-Family Building
Authors: Mylonas Angelos , Macià-Cid Jordi , Péan Thibault Q. , Grigoropoulos Nasos , Christou Ioannis T. , Pascual Jordi , Salom Jaume
Published: October 2024

The Energy Performance of Buildings Directive (EPBD) has set a target to achieve carbon-neutral building stock and generate 80% of its electricity from renewable sources by 2050. While Model Predictive Control (MPC) can contribute significantly to energy flexibility in buildings, its remote implementation remains relatively unexplored, especially in the residential sector. The purpose of this research is to demonstrate the reliability, robustness, and computational efficiency of a cloud-based application of an MPC called Smart Energy Management (SEM) on a multi-family residential building. The SEM was tested on a virtual building model in TRNSYS using an open-source distributed event streaming platform for data exchange and synchronization. Simplified models for thermal behavior prediction, including an R3C3 model of the building, were developed in C++. The SEM was evaluated in eight scenarios with varying weather conditions, optimization criteria, and runtime periods. The results demonstrate that the SEM maintains stability and robustness over a 2-week period with a 15-minute planning resolution while ensuring thermal comfort. The C++ implementation of the optimization algorithm enables SEM deployment on low-spec servers, supporting cost-effective applications in real buildings with minimal intervention.
Validation of a Model Predictive Control Strategy on a High Fidelity Building Emulator
Authors: Fop Davide , Yaghoubi Ali Reza , Capozzoli Alfonso
Published: October 2024

In recent years, advanced controllers, including Model Predictive Control (MPC), have emerged as promising solutions to improve the efficiency of building energy systems. This paper explores the capabilities of MPC in handling multiple control objectives and constraints. A first MPC controller focuses on the task of ensuring thermal comfort in a residential house served by a heat pump while minimizing the operating costs when subject to different pricing schedules. A second MPC controller working on the same system tests the ability of MPC to deal with demand response events by enforcing a time-varying maximum power usage limitation signal from the electric grid. Furthermore, multiple combinations of the control parameters are tested in order to assess their influence on the controller performance. The controllers are tested on the BOPTEST framework, which offers standardized test cases in high-fidelity emulation models, and pre-defined baseline control strategies to allow fair comparisons also across different studies. Results show that MPC is able to handle multi-objective optimal control problems, reducing thermal comfort violations by between 66.9% and 82% and operational costs between 15.8% up to 20.1%, depending on the specific scenario analyzed. Moreover, MPC proves its capability to exploit the building thermal mass to shift heating power consumption, allowing the latter to adapt its time profile to time-varying constraints. The proposed methodology is based on technologically feasible steps that are intended to be easily transferred to large scale, in-field applications.
Detection of Impedance Inhomogeneity in Lithium-Ion Battery Packs Based on Local Outlier Factor
Authors: Zhu Lijun , Wang Jian , Wang Yutao , Pan Bin , Wang Lujun
Published: October 2024

The inhomogeneity between cells is the main cause of failure and thermal runaway in Lithium-ion battery packs. Electrochemical Impedance Spectroscopy (EIS) is a non-destructive testing technique that can map the complex reaction processes inside the battery. It can detect and characterise battery anomalies and inconsistencies. This study proposes a method for detecting impedance inconsistencies in Lithium-ion batteries. The method involves conducting a battery EIS test and Distribution of Relaxation Times (DRT) analysis to extract characteristic frequency points in the full frequency band. These points are less affected by the State of Charge (SOC) and have a strong correlation with temperature, charge/discharge rate, and cycles. An anomaly detection characteristic impedance frequency of 136.2644 Hz was determined for a cell in a Lithium-ion battery pack. Single-frequency point impedance acquisition solves the problem of lengthy measurements and identification of anomalies throughout the frequency band. The experiment demonstrates a significant reduction in impedance measurement time, from 1.05 h to just 54 s. The LOF was used to identify anomalies in the EIS data at this characteristic frequency. The detection results were consistent with the actual conditions of the battery pack in the laboratory, which verifies the feasibility of this detection method. The LOF algorithm was chosen due to its superior performance in terms of FAR (False Alarm Rate), MAR (Missing Alarm Rate), and its fast anomaly identification time of only 0.1518 ms. The method does not involve complex mathematical models or parameter identification. This helps to achieve efficient anomaly identification and timely warning of single cells in the battery pack.
Assessing the Impact of Urbanization and Climate Change on Hydrological Processes in a Suburban Catchment
Authors: Kimbi Sharon Bih , Onodera Shin-ichi , Wang Kunyang , Kaihotsu Ichirow , Shimizu Yuta
Published: October 2024

Global urbanization, population growth, and climate change have considerably impacted water resources, making sustainable water resource management (WRM) essential. Understanding the changes in hydrological components is important for effective WRM, particularly in cities such as Higashi-Hiroshima, which is known for its saké brewing industry. This study used the Soil and Water Assessment Tool (SWAT) with Hydrological Response Units (HRUs) to achieve high spatial precision in assessing the impacts of land use change and climate variability on hydrological components in a suburban catchment in western Japan. Over the 30-year study period (1980s–2000s), land use change was the main driver of hydrological variability, whereas climate change played a minor role. Increased surface runoff, along with decrease in groundwater recharge, evapotranspiration, and baseflow, resulted in an overall reduction in water yield, with a 34.9% decrease in groundwater recharge attributed to the transformation of paddy fields into residential areas. Sustainable WRM practices, including water conservation, recharge zone protection, and green infrastructure, are recommended to balance urban development with water sustainability. These findings offer valuable insights into the strategies for managing water resources in rapidly urbanizing regions worldwide, emphasizing the need for an integrated WRM system that considers both land use and climate change impacts.
Exploring Opportunities and Challenges: SWOT Analysis for Advancing Smart Tech Solutions in Managing Lymantria dispar dispar Infestations in Forests of the European Union
Authors: Trigkas Sotirios J. , Eleftheriadou Nikoleta , Boukouvala Maria C. , Skourti Anna , Koukouli Maria , Kavallieratos Nickolas G.
Published: October 2024

The European spongy moth, Lymantria dispar dispar (L.) (Lepidoptera: Erebidae), originating from Eurasia, is found in Europe, Africa, and North America. Its polyphagous larvae infest deciduous and coniferous trees, causing severe damage during mass outbreak years. Thus, it is listed as one of the top 100 invasive alien species worldwide. The management and containment of this pest vary significantly between Europe and North America, with North America exhibiting a more robust response regarding the containment of the outbreaks. This study evaluates the current state of the European Union (EU-27) forests’ legal, political and cooperative protection frameworks concerning L. dispar dispar. We identified active and potential new stakeholders to assess the level of national and international collaboration in forest protection. We conducted a SWOT analysis to propose new strategies and solutions, aiming for enhanced cooperation in protecting EU forests from L. dispar dispar outbreaks. Our findings highlight the potential of new monitoring and reporting technologies and the importance of increased social and political awareness through social media and public campaigns. These measures would enable more centralized and coordinated efforts among member states. A few of the most significant results in each category of SWOT analysis are as follows: for strengths, a well-established network of EU and national stakeholders exists; for opportunities, emerging innovative technologies, such as IoT, AI, and 5G, are transforming our approach to forest protection; for weaknesses, there is a lack of informed choices regarding proactive measures to contain the outbreak due to a lack of centralized EU coordination and the inefficiencies of national state policies. Finally, the numerous threats to the well-being of EU forests competing for the attention of institutions and relevant stakeholders is by far one of the most important parameters regarding the threats to the EU’s forest protection. The study advocates for a unified, technologically advanced approach to manage and mitigate L. dispar dispar impacts in European forests, emphasizing the need for strengthened international cooperation and the strategic implementation of innovative solutions.
Differences in COVID-19 Vaccination and Experiences among Patients with Hypertension in Colombia and Jamaica during the COVID-19 Pandemic
Authors: Duncan Jacqueline P. , Geng Siyi , Lindsay Carene , Ferguson Trevor S. , Mills Katherine T. , Lopez-Lopez Jose Patricio , He Hua , Lanza Paola , Marshall Allison N. , Williams Makeda J. , Tonwe Veronica , Reyes Mabel , Campo Alfonso , Lopez-Jaramillo Patricio , Tulloch-Reid Marshall K.
Published: October 2024

During the COVID-19 pandemic, hypertensive patients had increased infection and healthcare disruption in many low- and middle-income countries (LMICs) with limited vaccine access. The objective of this report is to describe COVID-19 experiences and vaccination uptake among hypertensive patients in Colombia and Jamaica. A cross-sectional study of patients with hypertension was conducted in primary care clinics in both countries between 2021 and 2022. Trained interviewers used a telephone-administered questionnaire to assess COVID-19 experiences (infection, vaccination, and healthcare access). A total of 576 patients (68.5% female, mean age: 67.5 years) participated. Health service disruption affecting access to care was low (<10%). Compared to Jamaica, more participants from Colombia reported testing positive for COVID-19, having family members or friends testing positive, losing family members or friends due to COVID-19, and being vaccinated. In logistic regression models, adjusted for age, sex, education, and rural/urban clinic status, fear of COVID-19 (OR 2.7, 95% CI: 1.2–6.1) and residence in Colombia (OR 5.9, 95% CI: 2.4–14.6) were associated with higher vaccination uptake. Disparities in access to COVID-19 testing and diagnosis may have influenced these country differences including fear of COVID-19 and vaccine uptake. Other factors need to be better understood to prepare for future pandemic responses.
Improving Mental Health Knowledge and Reducing Mental Health Stigma Among Public Safety Personnel: Comparison of Live vs. Online Psychoeducation Training Programs
Authors: Marks Madeline R. , Bowers Clint , Beidel Deborah C. , Ortman Jordan , Newins Amie R.
Published: October 2024

This study evaluates the effectiveness of a brief psychoeducation training program in reducing mental health stigma, both toward others and oneself, among public safety personnel, while also comparing the relative effectiveness of in-person and online training modalities. In total, 1686 public safety personnel in Florida received psychoeducation on the mental health impacts of public safety work. Participants completed pre- and post-training questionnaires assessing demographics, mental health knowledge, and mental health stigma toward others and themselves. Among the participants, 871 completed the training online, and 44 completed the training in-person. A paired samples t-test assessed changes in knowledge from pre- to post-test, and 2 × 2 repeated measures ANOVAs analyzed stigma-related data. Mental health knowledge increased and mental health stigma toward others decreased post-training, with no differences between training modalities. No changes in mental health self-stigma were found. Police officers reported significantly greater self-stigma than firefighters. Firefighters and dispatchers reported significantly less stigma toward others than police officers. This study found that both in-person and online psychoeducation can similarly improve mental health knowledge and reduce stigma toward others, which may help reduce barriers to seeking care.
Effects of Differentially Methylated CpG Sites in Enhancer and Promoter Regions on the Chromatin Structures of Target LncRNAs in Breast Cancer
Authors: Fan Zhiyu , Chen Yingli , Yan Dongsheng , Li Qianzhong
Published: October 2024

Aberrant DNA methylation plays a crucial role in breast cancer progression by regulating gene expression. However, the regulatory pattern of DNA methylation in long noncoding RNAs (lncRNAs) for breast cancer remains unclear. In this study, we integrated gene expression, DNA methylation, and clinical data from breast cancer patients included in The Cancer Genome Atlas (TCGA) database. We examined DNA methylation distribution across various lncRNA categories, revealing distinct methylation characteristics. Through genome-wide correlation analysis, we identified the CpG sites located in lncRNAs and the distally associated CpG sites of lncRNAs. Functional genome enrichment analysis, conducted through the integration of ENCODE ChIP-seq data, revealed that differentially methylated CpG sites (DMCs) in lncRNAs were mostly located in promoter regions, while distally associated DMCs primarily acted on enhancer regions. By integrating Hi-C data, we found that DMCs in enhancer and promoter regions were closely associated with the changes in three-dimensional chromatin structures by affecting the formation of enhancer–promoter loops. Furthermore, through Cox regression analysis and three machine learning models, we identified 11 key methylation-driven lncRNAs (DIO3OS, ELOVL2-AS1, MIAT, LINC00536, C9orf163, AC105398.1, LINC02178, MILIP, HID1-AS1, KCNH1-IT1, and TMEM220-AS1) that were associated with the survival of breast cancer patients and constructed a prognostic risk scoring model, which demonstrated strong prognostic performance. These findings enhance our understanding of DNA methylation’s role in lncRNA regulation in breast cancer and provide potential biomarkers for diagnosis.
Immune Checkpoint Inhibitor Therapy and Associations with Clonal Hematopoiesis
Authors: Singh Abhay , Trinchant Nuria Mencia , Mishra Rahul , Arora Kirti , Mehta Smit , Kuzmanovic Teodora , Zokaei Nikoo Maedeh , Singh Inderpreet , Przespolewski Amanda C. , Swaminathan Mahesh , Ernstoff Marc S. , Dy Grace K. , Yan Lunbiao , Sinha Eti , Sharma Shruti , Hassane Duane C. , Griffiths Elizabeth A. , Wang Eunice , Guzman Monica L. , Thota Swapna
Published: October 2024

Cancer cohorts are now known to be associated with increased rates of clonal hematopoiesis (CH). We sort to characterize the hematopoietic compartment of patients with melanoma and non-small cell lung cancer (NSCLC) given our recent population level analysis reporting evolving rates of secondary leukemias. The advent of immune checkpoint blockade (ICB) has dramatically changed our understanding of cancer biology and has altered the standards of care for patients. However, the impact of ICB on hematopoietic myeloid clonal expansion remains to be determined. We studied if exposure to ICB therapy affects hematopoietic clonal architecture and if their evolution contributed to altered hematopoiesis. Blood samples from patients with melanoma and NSCLC (n = 142) demonstrated a high prevalence of CH. Serial samples (or post ICB exposure samples; n = 25) were evaluated in melanoma and NSCLC patients. Error-corrected sequencing of a targeted panel of genes recurrently mutated in CH was performed on peripheral blood genomic DNA. In serial sample analysis, we observed that mutations in DNMT3A and TET2 increased in size with longer ICB exposures in the melanoma cohort. We also noted that patients with larger size DNMT3A mutations with further post ICB clone size expansion had longer durations of ICB exposure. All serial samples in this cohort showed a statistically significant change in VAF from baseline. In the serial sample analysis of NSCLC patients, we observed similar epigenetic expansion, although not statistically significant. Our study generates a hypothesis for two important questions: (a) Can DNMT3A or TET2 CH serve as predictors of a response to ICB therapy and serve as a novel biomarker of response to ICB therapy? (b) As ICB-exposed patients continue to live longer, the myeloid clonal expansion may portend an increased risk for subsequent myeloid malignancy development. Until now, the selective pressure of ICB/T-cell activating therapies on hematopoietic stem cells were less known and we report preliminary evidence of clonal expansion in epigenetic modifier genes (also referred to as inflammatory CH genes).
Advancing Treatment Options for Merkel Cell Carcinoma: A Review of Tumor-Targeted Therapies
Authors: Nammour Helena M. , Madrigal Karla , Starling Caroline T. , Doan Hung Q.
Published: October 2024

Although rare, Merkel cell carcinoma (MCC) is a highly aggressive and increasingly prevalent neuroendocrine cancer of the skin. While current interventions, including surgical resection, radiation, and immunotherapy have been employed in treating many patients, those who remain unresponsive to treatment are met with sparse alternatives and a grim prognosis. For this reason, it is of interest to expand the repertoire of available therapies for MCC patients who remain resistant to current primary interventions. Recently, our improved mechanistic understanding of aberrant cell signaling observed in both MCPyV-positive and -negative MCC has facilitated exploration into several small molecules and inhibitors, among them receptor tyrosine kinase inhibitors (TKIs) and somatostatin analogs (SSAs), both of which have positively improved response rates and reduced tumor volumes upon application to treatment of MCC. The introduction of such targeted therapies into treatment protocols holds promise for more personalized care tailored towards patients of diverse subtypes, thereby improving outcomes and mitigating tumor burden, especially for treatment-resistant individuals. In this review, we characterize recent findings surrounding targeted treatments that have been applied to MCC and provide an overview of emerging perspectives on translatable options that can be further developed to offer additional therapeutic avenues for patients with the disease.
Contrast Relative Humidity Response of Diverse Cowpea (Vigna unguiculata (L.) Walp.) Genotypes: Deep Study Using RNAseq Approach
Authors: Krylova Ekaterina A. , Burlyaeva Marina O. , Tvorogova Varvara E. , Khlestkina Elena K.
Published: October 2024

Cowpea (Vigna unguiculata (L.) Walp.) is appreciated for its suitability for cultivation and obtaining good yields in relatively extreme farming conditions. It is resistant to high temperatures and drought. Moreover, food products prepared from Vigna are rich in many nutrients such as proteins, amino acids, carbohydrates, minerals, fiber, vitamins, and other bioactive compounds. However, in East and Southeast Asia, where the products of this crop are in demand, the climate is characterized by excessive humidity. Under these conditions, the vast majority of cowpea varieties tend to have indeterminate growth (elongated shoot length) and are unsuitable for mechanized harvesting. The molecular mechanisms for tolerance to high relative humidity remain the least studied in comparison with those for other abiotic stress factors (drought, heat, cold, flooding, etc.). The purpose of the work was to reveal and investigate differentially expressed genes in cowpea accessions having contrasting growth habits (determinate and indeterminate) under humid and drought conditions. We performed RNA-seq analysis using selected cowpea accessions from the VIR collection. Among the genotypes used, some have significant changes in their plant architecture in response to high relative humidity, while others were tolerant to these conditions. In total, we detected 1697 upregulated and 1933 downregulated genes. The results showed that phytohormone-related genes are involved in cowpea response to high relative humidity. DEGs associated with jasmonic acid signaling are proposed to be key contributors in the maintenance of compact architecture under humid conditions.
‘Selected’ Exosomes from Sera of Elderly Severe Obstructive Sleep Apnea Patients and Their Impact on Blood–Brain Barrier Function: A Preliminary Report
Authors: Guillot Pauline , Celle Sebastien , Barth Nathalie , Roche Frederic , Perek Nathalie
Published: October 2024

Obstructive sleep apnea syndrome (OSAS) affects a large part of the aging population. It is characterized by chronic intermittent hypoxia and associated with neurocognitive dysfunction. One hypothesis is that the blood–brain barrier (BBB) functions could be altered by exosomes. Exosomes are nanovesicles found in biological fluids. Through the study of exosomes and their content in tau and amyloid beta (Aβ), the aim of this study was to show how exosomes could be used as biomarkers of OSAS and of their cognitive disorders. Two groups of 15 volunteers from the PROOF cohort were selected: severe apnea (AHI > 30) and control (AHI < 5). After exosome isolation from blood serum, we characterized and quantified them (CD81, CD9, CD63) by western blot and ELISAs and put them 5 h in contact with an in vitro BBB model. The apparent permeability of the BBB was measured using sodium-fluorescein and TEER. Cell ELISAs were performed on tight junctions (ZO-1, claudin-5, occludin). The amount of tau and Aβ proteins found in the exosomes was quantified using ELISAs. Compared to controls, OSAS patients had a greater quantity of exosomes, tau, and Aβ proteins in their blood sera, which induced an increase in BBB permeability in the model and was reflected by a loss of tight junction’ expression. Elderly patients suffering severe OSAS released more exosomes in serum from the brain compartment than controls. Such exosomes increased BBB permeability. The impact of such alterations on the risk of developing cognitive dysfunction and/or neurodegenerative diseases is questioned.
The Critical Role of Phenylpropanoid Biosynthesis Pathway in Lily Resistance Against Gray Mold
Authors: Cui Qi , Li Xinran , Hu Shanshan , Yang Dongfeng , Abozeid Ann , Yang Zongqi , Jiang Junhao , Ren Ziming , Li Danqing , Li Dongze , Zheng Liqun , Qin Anhua
Published: October 2024

Gray mold caused by Botrytis elliptica is one of the most determinative factors of lily growth and has become a major threat to lily productivity. However, the nature of the lily B. elliptica interaction remains largely unknown. Here, comparative transcriptomic and metabolomic were used to investigate the defense responses of resistant (‘Sorbonne’) and susceptible (‘Tresor’) lily cultivars to B. elliptica infection at 24 hpi. In total, 1326 metabolites were identified in ‘Sorbonne’ and ‘Tresor’ after infection, including a large number of phenylpropanoids. Specifically, the accumulation of four phenylpropanes, including eriodictyol, hesperetin, ferulic acid, and sinapyl alcohol, was significantly upregulated in the B. elliptica-infected ‘Sorbonne’ compared with the infected ‘Tresor’, and these phenylpropanes could significantly inhibit B. elliptica growth. At the transcript level, higher expression levels of F3′M, COMT, and CAD led to a higher content of resistance-related phenylpropanes (eriodictyol, ferulic acid, and sinapyl alcohol) in ‘Sorbonne’ following B. elliptica infection. It can be assumed that these phenylpropanes cause the resistance difference between ‘Sorbonne’ and ‘Tresor’, and could be the potential marker metabolites for gray mold resistance in the lily. Further transcriptional regulatory network analysis suggested that members of the AP2/ERF, WRKY, Trihelix, and MADS-M-type families positively regulated the biosynthesis of resistance-related phenylpropanes. Additionally, the expression patterns of genes involved in phenylpropanoid biosynthesis were confirmed using qRT-PCR. Therefore, we speculate that the degree of gray mold resistance in the lily is closely related to the contents of phenylpropanes and the transcript levels of the genes in the phenylpropanoid biosynthesis pathway. Our results not only improve our understanding of the lily’s resistance mechanisms against B. elliptica, but also facilitate the genetic improvement of lily cultivars with gray mold resistance.
Plasma Lipidomics Reveals Lipid Signatures of Early Pregnancy in Mares
Authors: Perera Tharangani R. W. , Bromfield Elizabeth G. , Gibb Zamira , Nixon Brett , Sheridan Alecia R. , Rupasinghe Thusitha , Skerrett-Byrne David A. , Swegen Aleona
Published: October 2024

Understanding the systemic biochemistry of early pregnancy in the mare is essential for developing new diagnostics and identifying causes for pregnancy loss. This study aimed to elucidate the dynamic lipidomic changes occurring during the initial stages of equine pregnancy, with a specific focus on days 7 and 14 post-ovulation. By analysing and comparing the plasma lipid profiles of pregnant and non-pregnant mares, the objective of this study was to identify potential biomarkers for pregnancy and gain insights into the biochemical adaptations essential for supporting maternal recognition of pregnancy and early embryonic development. Employing discovery lipidomics, we analysed plasma samples from pregnant and non-pregnant mares on days 7 and 14 post-conception using the SCIEX ZenoTOF 7600 system. This high-resolution mass spectrometry approach enabled us to comprehensively profile and compare the lipidomes across these critical early gestational timepoints. Our analysis revealed significant lipidomic alterations between pregnant and non-pregnant mares and between days 7 and 14 of pregnancy. Key findings include the upregulation of bile acids, sphingomyelins, phosphatidylinositols, and triglycerides in pregnant mares. These changes suggest enhanced lipid synthesis and mobilization, likely associated with the embryo’s nutritional requirements and the establishment of embryo–maternal interactions. There were significant differences in lipid metabolism between pregnant and non-pregnant mares, with a notable increase in the sterol lipid BA 24:1;O5 in pregnant mares as early as day 7 of gestation, suggesting it as a sensitive biomarker for early pregnancy detection. Notably, the transition from day 7 to day 14 in pregnant mares is characterized by a shift towards lipids indicative of membrane biosynthesis, signalling activity, and preparation for implantation. The study demonstrates the profound lipidomic shifts that occur in early equine pregnancy, highlighting the critical role of lipid metabolism in supporting embryonic development. These findings provide valuable insights into the metabolic adaptations during these period and potential biomarkers for early pregnancy detection in mares.
Peptide Activator Stabilizes DJ-1 Structure and Enhances Its Activity
Authors: Shih Jing-Yuan , Hsu Yuan-Hao Howard
Published: October 2024

DJ-1 is a vital enzyme involved in the maintenance of mitochondrial health, and its mutation has been associated with an increased risk of Parkinson’s disease (PD). Effective regulation of DJ-1 activity is essential for the well-being of mitochondria, and DJ-1 is thus a potential target for PD drug development. In this study, two peptides (15EEMETIIPVDVMRRA29 and 47SRDVVICPDA56) were utilized with the aim of enhancing the activity of DJ-1. The mechanisms underlying the activity enhancement by these two peptides were investigated using hydrogen/deuterium exchange mass spectrometry (HDXMS). The HDXMS results revealed distinct mechanisms. Peptide 1 obstructs the access of solvent to the dimer interface and stabilizes the α/β hydrolase structure, facilitating substrate binding to a stabilized active site. Conversely, peptide 2 induces a destabilization of the α/β hydrolase core, enhancing substrate accessibility and subsequently increasing DJ-1 activity. The binding of these two peptides optimizes the activity site within the dimeric structure. These findings offer valuable insights into the mechanisms underlying the activity enhancement of DJ-1 by the two peptides, potentially aiding the development of new drugs that can enhance the activity of DJ-1 and, consequently, advance PD treatment.
Double-Edged Sword Effect of Diet and Nutrition on Carcinogenic Molecular Pathways in Breast Cancer
Authors: Neagu Anca-Narcisa , Josan Claudiu-Laurentiu , Jayaweera Taniya M. , Weraduwage Krishan , Nuru Niyogushima , Darie Costel C.
Published: October 2024

Environmental exposure to a mixture of chemical xenobiotics acts as a double-edged sword, promoting or suppressing tumorigenesis and the development of breast cancer (BC). Before anything else, we are what we eat. In this review, we highlight both “the good” and “the bad” sides of the daily human diet and dietary patterns that could influence BC risk (BCR) and incidence. Thus, regularly eating new, diversified, colorful, clean, nutrient-rich, energy-boosting, and raw food, increases apoptosis and autophagy, antioxidation, cell cycle arrest, anti-inflammation, and the immune response against BC cells. Moreover, a healthy diet could lead to a reduction in or the inhibition of genomic instability, BC cell stemness, growth, proliferation, invasion, migration, and distant metastasis. We also emphasize that, in addition to beneficial compounds, our food is more and more contaminated by chemicals with harmful effects, which interact with each other and with endogenous proteins and lipids, resulting in synergistic or antagonistic effects. Thus, a healthy and diverse diet, combined with appropriate nutritional behaviors, can exert anti-carcinogenic effects and improve treatment efficacy, BC patient outcomes, and the overall quality of life of BC patients.
Comparative Analysis of Hepatic Gene Expression Profiles in Murine and Porcine Sepsis Models
Authors: Halimi Fëllanza , Vanderhaeghen Tineke , Timmermans Steven , Croubels Siska , Libert Claude , Vandewalle Jolien
Published: October 2024

Sepsis remains a huge unmet medical need for which no approved drugs, besides antibiotics, are on the market. Despite the clinical impact of sepsis, its molecular mechanism remains inadequately understood. Recent insights have shown that profound hepatic transcriptional reprogramming, leading to fatal metabolic abnormalities, might open a new avenue to treat sepsis. Translation of experimental results from rodents to larger animal models of higher relevance for human physiology, such as pigs, is critical and needs exploration. We performed a comparative analysis of the transcriptome profiles in murine and porcine livers using the following sepsis models: cecal ligation and puncture (CLP) in mice and fecal instillation (FI) in pigs, both of which induce polymicrobial septic peritonitis, and lipopolysaccharide (LPS)-induced endotoxemia in pigs, inducing sterile inflammation. Using bulk RNA sequencing, Metascape pathway analysis, and HOMER transcription factor motif analysis, we were able to identify key genes and pathways affected in septic livers. Conserved upregulated pathways in murine CLP and porcine LPS and FI generally comprise typical inflammatory pathways, except for ER stress, which was only found in the murine CLP model. Conserved pathways downregulated in sepsis comprise almost exclusively metabolic pathways such as monocarboxylic acid, steroid, biological oxidation, and small-molecule catabolism. Even though the upregulated inflammatory pathways were equally induced in the two porcine models, the porcine FI model more closely resembles the metabolic dysfunction observed in the CLP liver compared to the porcine LPS model. This comprehensive comparison focusing on the hepatic responses in mouse CLP versus LPS or FI in pigs shows that the two porcine sepsis models generally resemble quite well the mouse CLP model, with a typical inflammatory signature amongst the upregulated genes and metabolic dysfunction amongst the downregulated genes. The hepatic ER stress observed in the murine model could not be replicated in the porcine models. When studying metabolic dysfunction in the liver upon sepsis, the porcine FI model more closely resembles the mouse CLP model compared to the porcine LPS model.
Quantitative Trait Locus Mapping Combined with RNA Sequencing Identified Candidate Genes for Resistance to Powdery Mildew in Bitter Gourd (Momordica charantia L.)
Authors: Huang Rukui , Liang Jiazuo , Ju Xixi , Huang Yuhui , Huang Xiongjuan , Chen Xiaofeng , Liu Xinglian , Feng Chengcheng
Published: October 2024

Improving the powdery mildew resistance of bitter gourd is highly important for achieving high yield and high quality. To better understand the genetic basis of powdery mildew resistance in bitter gourd, this study analyzed 300 lines of recombinant inbred lines (RILs) formed by hybridizing the powdery mildew-resistant material MC18 and the powdery mildew-susceptible material MC402. A high-density genetic map of 1222.04 cM was constructed via incorporating 1,996,505 SNPs generated by resequencing data from 180 lines, and quantitative trait locus (QTL) positioning was performed using phenotypic data at different inoculation stages. A total of seven QTLs related to powdery mildew resistance were identified on four chromosomes, among which qPm-3-1 was detected multiple times and at multiple stages after inoculation. By selecting 18 KASP markers that were evenly distributed throughout the region, 250 lines and parents were genotyped, and the interval was narrowed to 207.22 kb, which explained 13.91% of the phenotypic variation. Through RNA-seq analysis of the parents, 11,868 differentially expressed genes (DEGs) were screened. By combining genetic analysis, gene coexpression, and sequence comparison analysis of extreme materials, two candidate genes controlling powdery mildew resistance in bitter gourd were identified (evm.TU.chr3.2934 (C3H) and evm.TU.chr3.2946 (F-box-LRR)). These results represent a step forward in understanding the genetic regulatory network of powdery mildew resistance in bitter gourd and lay a molecular foundation for the genetic improvement in powdery mildew resistance.
Alteration in sB7-H4 Serum Levels and Placental Biomarker Expression after Therapeutic Plasma Exchange in Early-Onset Preeclampsia Patients
Authors: Duan Liyan , Ma Yuyang , Reisch Beatrix , Hadrovic Elina , Mach Pawel , Kimmig Rainer , Jahn Michael , Köninger Angela , Iannaccone Antonella , Gellhaus Alexandra
Published: October 2024

Therapeutic plasma exchange (TPE) is a widely used treatment for numerous diseases including pregnancy-related conditions. Our prior study on 20 early-onset preeclampsia patients undergoing TPE revealed a significant extension in pregnancy duration and reduced serum levels of sFlt-1, sFlt-1/PlGF, and sEndoglin. Here, we investigated the impact of TPE on serum sB7-H4, an immunological checkpoint molecule, and placental proteins (Flt-1, Eng, B7-H4, iNOS, TNF-α) in TPE-treated early-onset preeclampsia patients (N = 12, 23 + 2–28 + 5 weeks), conventionally treated counterparts (N = 12, 23 + 5–30 weeks), and gestational age-matched controls (N = 8, 22 + 4–31 + 6 weeks). Immunoblotting, ELISA, and co-immunohistochemistry were used for biomarker analysis, including placental inflammation factors (iNOS, TNF-α). The results showed that TPE extended pregnancy by a median of 6.5 days in this cohort of early-onset preeclampsia. Serum sB7-H4, sFlt-1, and sEndoglin levels decreased, along with reduced expression of their membrane-bound proteins in placental tissue upon TPE treatment. Moreover, TPE-treated patients displayed reduced placental inflammation compared to preeclampsia patients receiving standard-of-care treatment. In conclusion, TPE may improve pregnancy outcomes in early-onset preeclampsia by lowering circulating levels of sB7-H4, sFlt-1, and sEndoglin, as well as reducing placental inflammation. This translational approach holds promise for enhancing placental function and extending gestation in high-risk pregnancies including very preterm PE or HELLP cases.
Discovery of Novel Thiazole-Based SIRT2 Inhibitors as Anticancer Agents: Molecular Modeling, Chemical Synthesis and Biological Assays
Authors: Piacente Francesco , Guccione Giorgia , Scarano Naomi , Lunaccio Dario , Miro Caterina , Abbotto Elena , Salis Annalisa , Tasso Bruno , Dentice Monica , Bruzzone Santina , Cichero Elena , Millo Enrico
Published: October 2024

The search and development of effective sirtuin small molecule inhibitors (SIRTIs) continues to draw great attention due to their wide range of pharmacological applications. Based on SIRTs’ involvement in different biological pathways, their ligands were investigated for many diseases, such as cancer, neurodegenerative disorders, diabetes, cardiovascular diseases and autoimmune diseases. The elucidation of a substantial number of SIRT2–ligand complexes is steering the identification of novel and more selective modulators. Among them, SIRT2 in the presence of the SirReal2 analog series was the most studied. On this basis, we recently reported structure-based analyses leading to the discovery of thiazole-based compounds acting as SIRT2 inhibitors (T1, SIRT2 IC50 = 17.3 µM). Herein, ligand-based approaches followed by molecular docking simulations allowed us to evaluate in silico a novel small series of thiazoles (3a–3d and 5a, 5d) as putative SIRT2 inhibitors. Results from the computational studies revealed comparable molecular interaction fields (MIFs) and docking positionings of most of these compounds with respect to reference SIRT2Is. Biochemical and biological assays validated this study and pointed to compound 5a (SIRT2 IC50 = 9.0 µM) as the most interesting SIRT2I that was worthy of further development as an anticancer agent.
Comprehensive Analysis of BrDUF506 Genes Across the Brassica rapa Genome Uncovers Potential Functions in Sexual Reproduction and Abiotic Stress Tolerance
Authors: Zhu Guangqi , Wang Jingxuan , He Shuang , Liang Kexin , Zhang Renyi , Huang Jiabao , Yang Xueqin , Zhang Xiaojing
Published: October 2024

The Domain of Unknown Function 506 (DUF506) belongs to the PD-(D/E) XK nuclease superfamily and has been reported to play critical roles in growth and development as well as responses to abiotic stresses. However, the function of DUF506 genes in Brassica rapa (B. rapa) remains unclear. In this study, a total of 18 BrDUF506 genes were identified and randomly distributed across eight chromosomes, categorized into four subfamilies. Analyzing their promoter sequences has uncovered various stress-responsive elements, such as those for drought, methyl jasmonate (MeJA), and abscisic acid (ABA). Bra000098 and Bra017099 exhibit significantly enhanced expression in response to heat and drought stress. Protein interaction predictions indicate that Bra000098 homolog, At2g38820, is interacting with ERF012 and PUB48 and is involved in abiotic stress regulation. Furthermore, gene expression profiling has identified Bra026262 with a high expression level in flowers and significantly decreased in female sterile mutants. Protein interaction prediction further revealed that its homolog, At4g32480, interacts with MYB and AGL proteins, suggesting the potential roles in female gametophyte development. The current study enhances our understanding of the functional roles of BrDUF506s, providing significant insights that are valuable in investigating sexual reproduction and abiotic stress responses in B. rapa.
Structural Evolution of Olivine during Mechanochemically Assisted Mineral Carbonation under CO2 Flow
Authors: Cau Costantino , Taras Alessandro , Masia Gabriele , Caggiu Laura , Enzo Stefano , Garroni Sebastiano , Murgia Fabrizio , Mulas Gabriele
Published: October 2024

The mechanism of the mechanically assisted mineral carbonation of commercial olivine under the flow of a carbon dioxide (CO2)/nitrogen (N2) mixture has been elucidated by ex situ powder X-ray diffraction and Fourier-transform infrared spectroscopy. The overall CO2 conversion depends on the rotational frequency of the mill’s engine, and it reaches 85% within 90 min of mechanical treatment at a flow rate of 2.5 L min−1. By tuning the frequency of rotation, the kinetics of CO2 conversion unveil a complex reaction pathway involving subsequent steps. Structural analyses suggest that clinochlore, a magnesium (Mg-)- and iron (Fe-)-containing aluminosilicate gathered among the components of olivine, is formed and consumed in different stages, thus promoting the CO2 sequestration that eventually results in the formation of hydrated and anhydrous Mg-based carbonates.
Becoming a “Hungry Mind”: Stability and Change in Need for Cognition across Adolescence
Authors: Lavrijsen Jeroen , Aerts Evelien , Preckel Franzis , Ramos Alicia , Verschueren Karine
Published: October 2024

Need for Cognition has been established as a key intellectual investment trait shaping students’ academic development. However, little is yet known about its malleability, particularly in youth. This study investigated stability and change in Need for Cognition in a large longitudinal sample of 3409 adolescents from 166 classes in 27 schools in Flanders. Participants reported on their Need for Cognition in Grades 7, 8, 11 and 12. First, the latent rank-order stability of Need for Cognition between Grades 7 and 11 was found to be r = 0.50. This stability was of a similar magnitude to that of the Big Five personality traits observed in the same sample and larger than that of academic intrinsic motivation. Second, trajectories of Need for Cognition over time were found to vary between individuals. In particular, three trajectory classes could be identified, differentiated mainly by the initial level of Need for Cognition (i.e., a low, medium, and high trajectory). Finally, cognitive ability, cognitive stimulation at home, and parental autonomy support, but not parental educational level, were associated with higher odds of belonging to the high Need for Cognition trajectory and lower odds of belonging to the low Need for Cognition trajectory.
Unmanned Ship Collision Avoidance Action Plan Deduction Method under Man–Machine Interactive Negotiation in Collision Avoidance Scenarios
Authors: Zheng Jian , Liu Baoshuo , Li Yun , Huang Changhai
Published: October 2024

With the development of artificial intelligence technology, the future water traffic environment will present a new pattern of coexistence of manned ships and unmanned ships, because unmanned ships are different from manned ships in situation understanding, collision avoidance decision-making, and so on. Therefore, the obstacle avoidance planning between unmanned ships and manned ships becomes extremely complex, and collision avoidance behavior scheme deduction becomes a key step in solving the problems related to situation understanding and collision avoidance decision-making in collision avoidance scenarios. In this paper, the situation understanding of the pilot for different collision avoidance situations is integrated into the dynamic obstacle avoidance model, and an intelligent navigation collision avoidance system is proposed to assist in deducing the collision avoidance action plan of the unmanned ship in the man–machine coexistence scenario. The intelligent navigation collision avoidance system is divided into two parts, namely a ship situation understanding part and a ship obstacle avoidance part, wherein ship situation understanding is used for realizing the transition of the collision state of the unmanned ship in the deduction process by constructing a collision-state set and a behavior decision set by using a finite state machine (FSM). Regarding ship obstacle avoidance, ship velocity obstacle is calculated based on the reciprocal velocity obstacle method (RVO), and the collision avoidance action is selected by using the behavior decision generated by the FSM to realize the dynamic collision avoidance deduction of the unmanned ship. In this study, the validity and effectiveness of the intelligent navigation collision avoidance system proposed in this paper are verified by case studies in a variety of collision avoidance scenarios. The system successfully solves the problem of intelligent collision avoidance planning, provides reliable support for the intelligent collision avoidance of unmanned ships, provides a feasible solution for safety and efficiency in sea navigation, and provides a valuable reference for the design and development of future intelligent navigation collision avoidance systems for ships.
Atomically Dispersed High-Active Site Density Copper Electrocatalyst for the Reduction of Oxygen
Authors: Jiang Tao , Jiang Hongli , Wang Weibin , Mu Hao , Zhang Ying , Li Bo
Published: October 2024

Enlarging the M-Nx active-site density is an effective route to enhance the ORR performance of M-N-C catalysts. In this work, a single-atom catalyst Cu–N@Cu–N–C with enlarged Cu–N4 active site density was prepared by the second doping and pyrolysis (SDP) of Cu–N–C derived from Cu-doped zeolite imidazole frameworks. The half-wave potentials of Cu–N@Cu–N–C were measured as 0.85 V in alkaline electrolyte and 0.75 V in acidic media, which was 50 mV and 60 mV higher than that of Cu–N–C, respectively. N2 adsorption–desorption isotherm curves and corresponding pore distribution analysis were used to verify the successful filling of additional Cu and N in micropores of Cu–N–C after SDP. The obvious increase in Cu contents for Cu–N@Cu–N–C (1.92 wt%) compared with Cu–N–C (0.88 wt%) tested by ICP demonstrated the successful doping of Cu into Cu–N–C. XAFS analysis confirmed the presence of Cu–N4 single-atom active centers in Cu–N@Cu–N–C. The N 1 s high-resolution XPS results proved a great increase in Cu–N4 contents from 13.15% for Cu–N–C to 18.36% for Cu–N@Cu–N–C. The enhanced ORR performance of Cu–N@Cu–N–C was attributed to the enlargement of Cu–N4 active site density, providing an effective route for the preparation of efficient and low-cost ORR catalysts.
Friction Characteristics of Low and High Strength Steels with Galvanized and Galvannealed Zinc Coatings
Authors: Kim Ji-Young , Yoon Seung-Chae , Jin Byeong-Keuk , Jeon Jin-Hwa , Hyun Joo-Sik , Lee Myoung-Gyu
Published: October 2024

As vehicle body structures become stronger and part designs more complex for lightweight, controlling frictional properties in automotive press forming has gained critical importance. Friction, a key factor in formability, is influenced by variables such as contact pressure, sliding velocity, sheet strength, and coatings. This study investigates the friction characteristics of steels with tensile strengths of 340 MPa and 980 MPa, under galvanized (GI) and galvannealed (GA) zinc coatings. Experimental results reveal that asperity flattening, a significant factor in determining friction, increases with contact pressure normalized by tensile strength, particularly for GI-coated steels. However, the relationship between friction and surface flattening deviates from conventional expectations, with the friction coefficient initially rising with increased flattening area up to ~20% before decreasing as flattening progresses. These findings suggest that traditional empirical formulas may not fully capture friction behavior under specific conditions. By understanding this inflection point, where friction reduces under high contact pressure, the study provides valuable insights for optimizing formability and improving sheet metal forming processes, especially in scenarios where precise friction control is critical for producing high-quality automotive parts.