bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 4 from ZEB1 Is Regulated by K811 Acetylation to Promote Stability, NuRD Complex Interactions, EMT, and NSCLC Metastasis
Authors: Perez-Oquendo Mabel , Manshouri Roxsan , Tian Yanhua , Fradette Jared J. , Rodriguez B. Leticia , Kundu Samrat T. , Gibbons Don L.
Published: August 2023

qPCR Primer Sequences
Supplementary Table 2 from ZEB1 Is Regulated by K811 Acetylation to Promote Stability, NuRD Complex Interactions, EMT, and NSCLC Metastasis
Authors: Perez-Oquendo Mabel , Manshouri Roxsan , Tian Yanhua , Fradette Jared J. , Rodriguez B. Leticia , Kundu Samrat T. , Gibbons Don L.
Published: August 2023

Potential ZEB1 acetylation-dependent target genes.
Supplementary Figures 1-8 from ZEB1 Is Regulated by K811 Acetylation to Promote Stability, NuRD Complex Interactions, EMT, and NSCLC Metastasis
Authors: Perez-Oquendo Mabel , Manshouri Roxsan , Tian Yanhua , Fradette Jared J. , Rodriguez B. Leticia , Kundu Samrat T. , Gibbons Don L.
Published: August 2023

S1. K811 acetylation is integral to ZEB1 molecular weight. S2. &tilde225 kDa ZEB1 band regulates protein stability. S3. K811 acetylation facilitates ZEB1/NuRD interaction. S4. K811 acetylation directs the ZEB1/NuRD complex to bind at genomic promoters. S5. K811 acetylation decreased epithelial expression levels. S6. RNA-seq reveals K811 acetylation downregulates the expression of epithelial genes. S7. K811 acetylation in ZEB1 KO cells promotes lung adenocarcinoma migration and invasion. S8. K811 acetylation enhances metastatic potential of murine NSCLC cells.
Supplementary Data from MDM2 Inhibition Enhances Immune Checkpoint Inhibitor Efficacy by Increasing IL15 and MHC Class II Production
Authors: Langenbach Marlene , Giesler Sophie , Richtsfeld Stefan , Costa-Pereira Sara , Rindlisbacher Lukas , Wertheimer Tobias , Braun Lukas M. , Andrieux Geoffroy , Duquesne Sandra , Pfeifer Dietmar , Woessner Nadine M. , Menssen Hans D. , Taromi Sanaz , Duyster Justus , Börries Melanie , Brummer Tilman , Blazar Bruce R. , Minguet Susana , Turko Patrick , Levesque Mitchell P. , Becher Burkhard , Zeiser Robert
Published: August 2023

Supplementary Figures Legends and Methods
Figure S5 from A Comparative Study of Neuroendocrine Heterogeneity in Small Cell Lung Cancer and Neuroblastoma
Authors: Cai Ling , DeBerardinis Ralph J. , Xie Yang , Minna John D. , Xiao Guanghua
Published: August 2023

A meta-analysis example that summarizes the association between MEK inhibitor resistance and NE scores.
Figure 7 from A Comparative Study of Neuroendocrine Heterogeneity in Small Cell Lung Cancer and Neuroblastoma
Authors: Cai Ling , DeBerardinis Ralph J. , Xie Yang , Minna John D. , Xiao Guanghua
Published: August 2023

Similar and distinct NE score–associated gene dependencies in SCLC and neuroblastoma cell lines. Selection of SCLC (A) and neuroblastoma (B) vulnerabilities based on the consistency (positive correlation) between CRISPR and RNAi data, and anticorrelation between dependency data and gene expression data. Pearson correlation coefficients from RNAi-CRISPR (left), RNAi-RNA expr (middle), and CRISPR-RNA expr (right) correlations were computed for all genes. The distributions of these coefficients are plotted as diagonal panels; pairwise correlations among these three sets of correlation coefficients were visualized as scatter plots in the lower triangular panels and the Pearson correlation coefficients are printed in the upper triangular panels. The four SCLC subtype driver TFs and the neuroblastoma oncogenic driver MYCN all have high consistency between CRISPR and RNAi data and high anticorrelation between dependency data and gene expression data. Areas with r > 0.4 from RNAi-CRISPR correlation, and r < −0.4 from RNAi-RNA expr and CRISPR-RNAi correlation were demarcated by light gray squares. C, Correlation between NE scores and effect scores of selected dependencies in SCLC and neuroblastoma. The upper part of the heat map displays selected vulnerabilities for SCLC and was ordered by correlations between NE scores and the effect scores in SCLC cell lines; likewise, the lower part of the heat map displays selected vulnerabilities for neuroblastoma. Genes with magenta arrows are showcased in D–I. Cell lines are ordered by their NE scores and annotated with NE score and SCLC driver TF expression. D–I, Comparison of selected gene dependencies in SCLC and neuroblastoma. In each plot, variable names are shown in the diagonal boxes, and scatter plots display relationships between each pairwise combination of variables. Lower triangular plots are colored by NE scores whereas upper triangular plots for SCLC figures are colored by TF classes. Pearson correlation coefficients are provided in lower triangular boxes for SCLC and upper triangular boxes for neuroblastoma. Refer to legends in C for color annotations.
Figure 4 from A Comparative Study of Neuroendocrine Heterogeneity in Small Cell Lung Cancer and Neuroblastoma
Authors: Cai Ling , DeBerardinis Ralph J. , Xie Yang , Minna John D. , Xiao Guanghua
Published: August 2023

NE score association with members of the Myc oncogene family in SCLC and neuroblastoma. Copy number (A) and RNA expression (B) of Myc family genes in SCLC and neuroblastoma cell lines. Note that although MYC amplification was higher in the high-NE-score SCLC cell lines, its gene expression was higher in the low-NE-score cell lines for both SCLC and neuroblastoma lines. Frequent MYCL loss was found in neuroblastoma because MYCL is located in a frequently deleted region (chromosome 1p) in neuroblastoma. C, NE score versus Myc gene member expression in SCLC studies. “UTSW cell line” is a cell line dataset; “Drapkin_2018” is a PDX dataset; “Rudin_2012,” “George_2015,” “Jiang_2016,” and “Cai_2021” are all patient tumor datasets. NE score versus Myc gene member expression in neuroblastoma cell line datasets (D) and tumor datasets (E). Note that some of the same cell lines were profiled in multiple studies. F, Forest plots visualizing meta-analysis of NE score association with Myc family genes. MYC expression is consistently associated with lower NE scores in SCLC and neuroblastoma samples (left). MYCL expression positively correlates with NE scores in SCLC but not neuroblastoma samples (middle). MYCN expression positively correlates with NE scores in neuroblastoma but not SCLC samples. G, Relationship between MYC copy number and gene expression in neuroblastoma and SCLC cell lines. Note that MYC amplification is only observed in SCLC cell lines. H,MYCN gene expression positively correlate with NE scores while controlling for MYCN amplification status in neuroblastoma patient tumors.
Figure 2 from A Comparative Study of Neuroendocrine Heterogeneity in Small Cell Lung Cancer and Neuroblastoma
Authors: Cai Ling , DeBerardinis Ralph J. , Xie Yang , Minna John D. , Xiao Guanghua
Published: August 2023

Inter- and intra-cell line NE heterogeneity. A, Inter-cell line NE heterogeneity. NE scores for CCLE pan-cancer cell lines were ranked from high to low. SCLC and neuroblastoma lines were highlighted by colors. Although most of the SCLC and neuroblastoma lines have high NE scores, a few of them also have low NE scores. B, Consistent gene expression pattern for SCLC NE signature genes observed for SCLC and neuroblastoma cell lines. Cell lines are in columns. Red/blue column left to the heat map annotates the correlation between the gene expression and NE score; the expression of SCLC driver TFs and NE scores was annotated above the heat map. SCLC lines were further classified into four TF classes. C, Average NE scores from scRNA-seq data align well with NE scores from bulk RNA-seq data for pan-cancer cell lines. D, Distribution of NE scores for lung cancer and neuroblastoma cell line–based scRNA-seq data. Intra-cell line NE heterogeneity. High- and low-NE score cells are found to coexist within the same SCLC cell line NCI-H1048 (E) or neuroblastoma cell line SKNAS (F). Single cells are in columns. Because of the high dropout rate of scRNA-seq data, only the top abundantly expressed genes are visualized.
Data from Desmoglein 2 Functions as a Receptor for Fatty Acid Binding Protein 4 in Breast Cancer Epithelial Cells
Authors: Chen Dongmei , Wirth Keith M. , Kizy Scott , Muretta Joseph M. , Markowski Todd W. , Yong Peter , Sheka Adam , Abdelwahab Hisham , Hertzel Ann V. , Ikramuddin Sayeed , Yamamoto Masato , Bernlohr David A.
Published: August 2023

Abstract
Fatty acid binding protein 4 (FABP4) is a secreted adipokine linked to obesity and progression of a variety of cancers. Obesity increases extracellular FABP4 (eFABP4) levels in animal models and in obese breast cancer patients compared with lean healthy controls. Using MCF-7 and T47D breast cancer epithelial cells, we show herein that eFABP4 stimulates cellular proliferation in a time and concentration dependent manner while the non-fatty acid-binding mutant, R126Q, failed to potentiate growth. When E0771 murine breast cancer cells were injected into mice, FABP4 null animals exhibited delayed tumor growth and enhanced survival compared with injections into control C57Bl/6J animals. eFABP4 treatment of MCF-7 cells resulted in a significant increase in phosphorylation of extracellular signal-regulated kinase 1/2 (pERK), transcriptional activation of nuclear factor E2-related factor 2 (NRF2) and corresponding gene targets ALDH1A1, CYP1A1, HMOX1, SOD1 and decreased oxidative stress, while R126Q treatment did not show any effects. Proximity-labeling employing an APEX2–FABP4 fusion protein revealed several proteins functioning in desmosomes as eFABP4 receptor candidates including desmoglein (DSG), desmocollin, junction plankoglobin, desomoplankin, and cytokeratins. AlphaFold modeling predicted an interaction between eFABP4, and the extracellular cadherin repeats of DSG2 and pull-down and immunoprecipitation assays confirmed complex formation that was potentiated by oleic acid. Silencing of DSG2 in MCF-7 cells attenuated eFABP4 effects on cellular proliferation, pERK levels, and ALDH1A1 expression compared with controls.
Implications:
These results suggest desmosomal proteins, and in particular desmoglein 2, may function as receptors of eFABP4 and provide new insight into the development and progression of obesity-associated cancers.
Supplementary Table S2 from Progressive Spreading of DNA Methylation in the GSTP1 Promoter CpG Island across Transitions from Precursors to Invasive Prostate Cancer
Authors: Gupta Harshath , Inoue Hitoshi , Nakai Yasutomo , Nakayama Masashi , Jones Tracy , Hicks Jessica L. , Kumar Balasubramanian , Gurel Meltem , Nelson William G. , De Marzo Angelo M. , Yegnasubramanian Srinivasan
Published: August 2023

Supplementary Table S2 shows the phased methylation of the GSTP1 promoter region across 102 lesions classified by density of methylation.
Supplementary Table S1 from Progressive Spreading of DNA Methylation in the GSTP1 Promoter CpG Island across Transitions from Precursors to Invasive Prostate Cancer
Authors: Gupta Harshath , Inoue Hitoshi , Nakai Yasutomo , Nakayama Masashi , Jones Tracy , Hicks Jessica L. , Kumar Balasubramanian , Gurel Meltem , Nelson William G. , De Marzo Angelo M. , Yegnasubramanian Srinivasan
Published: August 2023

Supplementary Table S1 shows patient and tumor characteristics from the patients who underwent radical prostatectomies for this study.
Supplementary Figure S2 from Progressive Spreading of DNA Methylation in the GSTP1 Promoter CpG Island across Transitions from Precursors to Invasive Prostate Cancer
Authors: Gupta Harshath , Inoue Hitoshi , Nakai Yasutomo , Nakayama Masashi , Jones Tracy , Hicks Jessica L. , Kumar Balasubramanian , Gurel Meltem , Nelson William G. , De Marzo Angelo M. , Yegnasubramanian Srinivasan
Published: August 2023

Supplementary Figure S2 shows the methylation rate by allele of the GSTP1 promoter region for partially methylated PIN alleles and demonstrates a hotspot of methylation around transcription factor binding sites and the transcription start site.
Supplementary Figure S1 from Progressive Spreading of DNA Methylation in the GSTP1 Promoter CpG Island across Transitions from Precursors to Invasive Prostate Cancer
Authors: Gupta Harshath , Inoue Hitoshi , Nakai Yasutomo , Nakayama Masashi , Jones Tracy , Hicks Jessica L. , Kumar Balasubramanian , Gurel Meltem , Nelson William G. , De Marzo Angelo M. , Yegnasubramanian Srinivasan
Published: August 2023

Supplementary Figure S1 shows the phased methylation by allele of the GSTP1 promoter region classified by density of methylation.
Supplemental Table 1 from Polymorphisms in the Nonhomologous End-joining DNA Repair Pathway are Associated with HPV Integration in Cervical Dysplasia
Authors: Geris Jennifer M. , Amirian E. Susan , Marquez-Do Deborah A. , Guillaud Martial , Dillon Laura M. , Follen Michele , Scheurer Michael E.
Published: August 2023

Supplemental Table 1: Tag SNPs and Calculated Odds Ratios
Supplementary Table S3 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Table S3 shows Total TILs and individual cell type vs TILs scores evaluated as a continuous variable and by median and Youden index cutoffs for BCR-free and metastasis-free survival.
Supplementary Table S1 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Table S1 shows the full list of differentially expressed genes in AA tumors along with log2 fold changes, p-values, and adjusted p-values.
Supplementary Figure S9 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S9 shows BCR-free and Metastasis-free survival for Total TILs scores dichotomized by median as well as Youden index cutoffs.
Supplementary Figure S7 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S7 shows non-significant relative cell type scores by Dx PSA Group.
Supplementary Figure S6 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S6 shows non-significant relative cell type scores by Gleason Sum.
Supplementary Figure S3 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S3 shows non-significant relative cell type scores by BCR status.
Supplementary Figure S11 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S11 shows BCR-free and Metastasis-free survival for cell types dichotomized by median cutoffs.
Supplementary Figure S10 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S10 shows BCR-free and Metastasis-free survival for top differentially expressed genes in AA tumors dichotomized by median cutoffs.
Supplementary Figure S1 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Supplementary Figure S1 shows differentially expressed gene pathways in AA tumors.
FIGURE 4 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Significant relative cell type scores for clinicopathologic variables. Relative cell type scores that demonstrated significance for GG (A), Gleason sum (B), metastasis (C), age at diagnosis group (D), and PSA (E) at diagnosis group are shown. Mann–Whitney U test (A, C) or Kruskal–Wallis test followed by Dunn multiple comparisons post-hoc test (B, D, E) were used to determine significance, as indicated by listed P values below 0.05. Each dot represents a patient score, and error bars correspond to mean ± SD.
FIGURE 3 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Immune cell profiling of prostate tumors based on clinicopathologic variables. Centered cell type scores are shown for race (A), BCR (B), GG (C), metastasis (D), Gleason sum (E), PSA at diagnosis (Dx PSA) group (F), and age (G) at diagnosis (Dx Age) group. H, Grid outlining cell type ratios for each clinicopathologic variable, based on Mann–Whitney U test (A–D) or Kruskal–Wallis test (E–G) at P values < 0.05 (blue), < 0.01 (red), and > 0.05 (gray).
FIGURE 2 from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Top differentially expressed immune-related genes associated with BCR-free and metastasis-free survival. Kaplan–Meier product-limit survival curves are plotted for differentially expressed genes, DVL2, KLRC2, LAIR2, ARG1, MAP3K5, and IFIH1, and their association with BCR-free (A–F) and metastasis-free survival (G–L). Significant genes are indicated by log-rank P value < 0.05.
Data from Immunologic Assessment of Tumors from a Race-matched Military Cohort Identifies Mast Cell Depletion as a Marker of Prostate Cancer Progression
Authors: Schafer Cara C. , Jiang Jiji , Elsamanoudi Sally , Nousome Darryl , Young Denise Y. , Song Yingjie , Sesterhenn Isabell A. , Chesnut Gregory T. , Tan Shyh-Han
Published: August 2023

Elucidating the cellular immune components underlying aggressive prostate cancer, especially among African American (AA) men who are disproportionately affected by this disease compared with Caucasian American (CA) men, will support more inclusive precision medicine treatment strategies. We aimed to evaluate which immune-related genes and cell types are differentially expressed in AA tumors and how immunobiology impacts prostate cancer progression.

We purified nucleic acid from tumor biopsies, obtained following radical prostatectomy, from 51 patients (AA = 26, CA = 25). Gene expression was measured using the NanoString platform from which we estimated immune cell abundances and assessed differences between groups based on clinicopathologic data. Product-limit estimates determined associations with biochemical recurrence (BCR)-free and metastasis-free survival.

DVL2 and KLRC2 were significantly upregulated in CA tumors and were also associated with worse disease progression. No significant differences in immune cell abundances by race were observed. Highly significant reductions in abundances of mast cells versus tumor-infiltrating lymphocytes (TIL) were found in men with high-grade pathologies and in men who later developed metastases. Low ratios of mast cells versus TILs were associated with worse BCR-free survival and metastasis-free survival.

Although estimated immune cell abundances were not different by race, we identified genes involved in metabolism and natural killer cell functions that were differentially expressed between AA and CA tumors. Among the entire cohort, depletion of mast cells within prostatectomy tumors was characteristic of advanced disease and susceptibility to disease progression.
Significance:
Our findings demonstrate that there are immune-related genes and pathways that differ by race. Impaired intratumoral cellular immune composition, especially for TIL-normalized mast cells, may be vital in predicting and contributing to prostate cancer disease progression.
Vibração das estruturas
Authors:   Universidade de São Paulo. Escola de Engenharia de São Carlos, Laier José Elias
Published: August 2023

Este texto é voltado para estudantes dos cursos de engenharia, especialmente de engenharia civil, bem como profissionais de engenharia em geral, tendo-se em mente que nos tempos atuais o conhecimento dos conceitos de dinâmica das estruturas vem ganhando importância crescente. O texto em questão é o resultado final do compromisso assumido pelo autor para com a Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Coordenação para o Aperfeiçoamento de Pessoal de Nível Superior, do Ministério da Educação e Cultura (CAPES-MEC), e Conselho Nacional de Desenvolvimento Científico e Tecnológico do Ministério da Ciência e Tecnologia (CNPq) em razão do apoio ao Programa de Pós-Doutorado do autor no Computational Mechanics Center de Southampton UK, no período de 1982 a 1983.
En búsqueda de un cine simbiótico: Shun de la ecuatoriana Sani Montahuano y Yollotl del mexicano Fernando Colin Roque
Authors: Paz y Miño Lucía Fernanda Romero
Published: August 2023

Este artículo desarrolla un análisis comparativo de los cortometrajes Shun (2021), de la cineasta ecuatoriana de nacionalidad indígena Sápara, Sani Montahuano, y Yollotl (2020) del cineasta mexicano Fernando Colin Roque.  El estudio se enmarca en las discusiones sobre el llamado giro ontológico desde la perspectiva del arte y la visualidad, y realiza su indagación desde una metodología visual crítica, que incluye un análisis composicional y otro interpretativo, a partir de la descripción de las películas y entrevistas a ambos autores. Se concluye que ambos cortometrajes representan la posibilidad de un cine simbiótico; uno cuya visualidad proponga una simbiosis inter-especies a partir de un tratamiento de la cámara que asuma diversas perspectivas corpóreas y sumergidas, mostrando la inter-representación entre humanos y no humanos. Siempre pensado como una potencialidad, este cine podría poner en relieve el pensamiento amerindio como posibilidad de respuesta ante la crisis climática que afecta particularmente a poblaciones y culturas empobrecidas y racializadas de América Latina.
Cymbidium × fugongense (Orchidaceae; Epidendroideae), a new natural hybrid from China: evidence from morphology and molecular analyses
Authors: KE SHIJIE , ZHANG CUILI , HUANG YE , ZHENG QIN-YAO , AHMAD SAGHEER , CHEN NANCHUAN , LAN SIREN , LIU ZHONGJIAN
Published: August 2023

This study illustrates and describes Cymbidium × fugongense, a natural hybrid of Cymbidium wenshanense and Cymbidium eburneum or Cymbidium maguanense, from Yunnan Province, China. Morphologically, it has white-pink sepals and petals; petals are mottled and contain purple-red patches at their centres. The midlobe of lip is purple-red, with occasionally purple-red mottling on the base and apex of side lobes. The column is purple-red and mottled ventrally. Molecular analysis of plastid DNA (matK and rbcL) sequence data confirmed the natural hybrid as related to C. wenshanense, whereas the nuclear (ITS) DNA clearly supported a relationship of this material to species of C. subgenus Cyperorchis.
Ostracods from the Kunming area of SW China, with description of two new species and male records of Cypridopsis vidua (O.F. Müller, 1776)
Authors: ZHAI DAYOU , FAN JIAWEI , WANG MIN
Published: August 2023

Previous studies of the Recent non-marine ostracods of Yunnan Province in southwestern China yielded 42 species, among which only 18 species were reported based on living material. Such a low number is likely to underestimate the species diversity of ostracods in Yunnan, considering the geographical disparity among its different parts. In this study, we report 11 nominal species and one species left in open nomenclature from 22 samples collected from the Kunming area in eastern-central part of Yunnan. Among these species, seven are new to the province, including Notodromas semiovata n. sp., Chrissia acuminata n. sp., and the first records of the male of Cypridopsis vidua (O.F. Müller, 1776) outside the USA. A review of previous literature indicates that, with the exception of the two new species, all the other 23 species have Palaearctic (PA) distributions, while only 14 have Oriental (OL) distributions. Such a composition contradicts previous vertebrate-based zoogeographical subdivisions of China in which Yunnan was included in the OL region, and adds to the argument that the geographical distributions of various invertebrate groups need to be investigated to map the PA‒OL boundary in China more appropriately.
First record of Anteon xericum Olmi & van Harten (Hymenoptera, Dryinidae) from Palaearctic region
Authors: MEIDANI ZAHRA , KHAYRANDISH MOHAMMAD , OLMI MASSIMO , MADJDZADEH SEYED MASSOUD , MOGHADDAM MOSTAFA GHAFOURI
Published: August 2023

The Malaise trap program was implemented to carry out periodic sampling in southeastern Iran, specifically targeting Kerman province, in order to investigate and study specimens belonging to the family Dryinidae. Among the assemblage of collected specimens, notable identifications were made within the subfamily Anteoninae, including the discernment of Anteon xericum Olmi & van Harten. This significant finding signifies a previously unreported occurrence in the Palaearctic region (Iran). A detailed diagnosis of the species, an identification key to Iranian Anteon species, a modified key to the Anteon females of the Eastern Palaearctic, along with a distribution map are provided.
4. The Organisation of Digitisation
Authors: Cöster Mathias , Danielson Mats , Ekenberg Love , Gullberg Cecilia , Titlestad Gard , Westelius Alf , Wettergren Gunnar
Published: August 2023

Whilst digitisation is far from a new concept, many assume that simply introducing automation and information systems in various forms will be enough to make their organisation’s operations more efficient. This misconception can often lead to disarray and costly mistakes. Digital Transformation: Understanding Business Goals, Risks, Processes, and Decisions shows how to avoid such issues via careful consideration of what an enterprise really needs.

Unlike many other books on digital transformation, the authors do not dwell on database design or the details of implementing information systems. Instead, they emphasise the importance of a clear understanding of all aspects of an organisation in order to effectively implement and manage digital systems, from business goals and strategies to structuring information and making decisions, risk assessments, project management, organising, and procuring services and products.

Organised in eleven chapters, and drawing on examples from all over the world, this book will be of interest to university students of business administration, management, information systems, and computer science, as well as practitioners seeking to better understand how to handle digital transformation in their own organisation.
7. Probability and Risk Management
Authors: Cöster Mathias , Danielson Mats , Ekenberg Love , Gullberg Cecilia , Titlestad Gard , Westelius Alf , Wettergren Gunnar
Published: August 2023

Whilst digitisation is far from a new concept, many assume that simply introducing automation and information systems in various forms will be enough to make their organisation’s operations more efficient. This misconception can often lead to disarray and costly mistakes. Digital Transformation: Understanding Business Goals, Risks, Processes, and Decisions shows how to avoid such issues via careful consideration of what an enterprise really needs.

Unlike many other books on digital transformation, the authors do not dwell on database design or the details of implementing information systems. Instead, they emphasise the importance of a clear understanding of all aspects of an organisation in order to effectively implement and manage digital systems, from business goals and strategies to structuring information and making decisions, risk assessments, project management, organising, and procuring services and products.

Organised in eleven chapters, and drawing on examples from all over the world, this book will be of interest to university students of business administration, management, information systems, and computer science, as well as practitioners seeking to better understand how to handle digital transformation in their own organisation.
Protective effect of nanoparticle NLRP3 inhibitor (MCC950) on cranial nerves in rats with vascular cognitive impairment
Authors: Shu Peng , Xiao Jie , Wu Bihua
Published: August 2023

Background: Chronic cerebral hypoperfusion (CCH) is a common cause of Vascular cognitive impairment (VCI). The neuroinflammation caused by ischemia play a key role in the progression of the ailment process. The over activation of NLRP3/Caspase-1/IL-1β signaling aggravates
the inflammatory response. We investigated whether NLRP3 inhibitors have a protective effect on cranial nerves in rats with VCI. Methods: Male SD rats were selected arbitrarily and categorized into the sham operation group, CCH group and CCH+MCC950 group (10 mg/kg). The rat model of
CCH was formed by bilateral common carotid artery occlusion (BCCAO). The changes in rat’s cerebral blood flow were observed by laser speckle imaging after operation. The sham operation and the CCH group were given intraperitoneal (i.p) injection of normal saline, and the therapeutic
group was given i.p having dose of MCC950 (10 mg/kg). The behavior of the rats was analyzed through the Y maze and open field experiments, and the hematoxylin-eosin (HE) staining technique was employed to analyze the behavior of the rats. The histological changes of the rat hippocampus were
observed under the following conditions, cell apoptosis was observed by Tune staining, and the protein expressions of NLRP3, IL (interleukin)-1β, and cleaved-Caspase-1 in the hippocampus were analyzed by Western blot. Results: MCC950 treatment enhanced the working memory
of CCH rats, and the rats in each group showed no motor deficit. As shown by HE staining, inhibition of NLRP3 reduced neuronal damage caused by pyroptosis. In addition, MCC950 diminishes the appearance of NLRP3, IL-1β, and cleaved-Caspase-1 in the brain tissue of CCH rats by inhibiting
inflammasome formation. Conclusions: inhibitors can improve the learning and cognitive ability of rats with vascular cognitive impairment, and the mechanism might be linked to inhibiting the NLRP3/Caspase-1/IL-1β signaling and decreasing apoptosis.
Preparation and characterization of biochar and its effects on soil properties, rhizosphere bacterial and tobacco yield with reduced compound fertilizer application
Authors: Ma Huan-Jin , Lin Li , Xu Sheng-Guang , Chen Ze-Bin , Fan Zhi-Wei , Jiang Ming-Xing , Li Yu
Published: August 2023

Biochar is known to have soil-improving effects and has been applied in agricultural production. Continuous cropping of flue-cured tobacco is common due to limited land resources, which can lead to soil obstacles that negatively affect yield. This study aimed to use agricultural waste
as raw material to prepare biochar, to improve soil and alleviate continuous cropping obstacles. A 7-year continuous cropping filed of flue-cured tobacco variety ‘yunyan 87′ in Luoshui Town was selected, with four treatments, compound fertilizer (T1) alone, and biochar with 100,
75 and 50% amount of compound fertilizer (T2, T3 and T4), respectively. The physicochemical properties of biochar were studied, its structure was observed under an electron microscope. Its effects with reduced compound fertilizer application on agronomic traits, tobacco yield, soil enzyme
activity and rhizosphere bacteria communities were investigated. In the results, biochar increased the tobacco yield (by 10.84%) compared to the same amount of fertilizer without biochar. Biochar application with 75% compound fertilizer (T3) could increase leaf area and yield of flue-cured
tobacco significantly. T3 treatment had little effect on the phenoloxidase activity in the rhizosphere soil, however it significantly enhanced the activities of peroxidase, protease and urease by 56.84%, 50% and 11.94% respectively, compared with the T1 treatment. The α-diversity
was the highest Under the T1 treatment. Biochar application (T2–T4) increased the soil pH significantly. Under biochar application with the decrease of compound fertilizer, the relative abundance of Proteobacteria improved whereas the abundance of unidentified_Bacteria, Chloroflexi and
Gemmatimonadetes decreased. Overall, the study provided evidence that the use of biochar with reduced amount of compound fertilizer could effectively improve the growth, soil quality and alleviate the negative effects of continuous cropping on tobacco. These findings provided a reference for
the potential use of agricultural waste and promoting sustainable crop production.
Ophiopogonin A attenuates acute lung inflammation in Klebsiella pneumonia mice by regulating RELA (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
Authors: Ying Xiwang , Leng Baolang , Feng Xia , Du Kang , Liu Zhengchuang
Published: August 2023

Ophiopogonin are natural products from the roots of Ophiopogon japonicas with various pharmacological activities. This study explored the mechanism of ophiopogonin A to alleviate the acute inflammation of the lungs of Klebsiella pneumonia. 64 rats were equally assigned
into blank control group, injury model group (intratracheal injection of Klebsiella pneumonia to establish Klebsiella pneumonia model), Ophiopogonin A group and Ophiopogonin A+RELA group (combined group, on the basis of injury model group, received RELA mediated ophiopogonin
A), with the dose of 10 mg/kg. The histopathological damage was observed under the microscope after HE staining, as well as inflammatory cells in bronchoalveolar lavage fluid (BALF), cytokine levels, and expression of RELA. After HE staining, compared with injury model group, bronchial epithelium
structure in Ophiopogonin A group and combination group were relatively intact, the degree of infiltration of inflammatory cells was reduced, and the thickness of alveolar septum was reduced, especially in combination group. The pathological scores of Ophiopogonin A group and combination group
were significantly lower, with a further lower score in combination group. The number of inflammatory cells in BALF of rats in Ophiopogonin A group and combination group were significantly reduced, especially in combination group. Similarly, cytokine levels in BALF supernatant and serum of
rats in treatment group were reduced, especially in combination group (P < 0.05). Ophiopogonin A could down-regulate RELA, which was further reduced in combination group. In conclusion, ophiopogonin A can reduce the invasion and pathological damage of pathogens to lung tissue and
exert an anti-inflammatory ability.
Effect of Nrf2/ARE signaling pathways on oxidative damage induced by sodium fluoride in rat osteoblasts
Authors: Wang Qian , Cai Jiping , Fan Jinpeng , Wang Na , Wang Xi , Li Xiaohua , Li Yun , Tian Xiaochen
Published: August 2023

To study the toxic mechanism of NaF on rat osteoblasts, the thiazole-blue tetrazole (MTT) method and alkaline phosphatase (ALP) method were used to evaluate the effect. Then, Comet assay was used to detect the effect of NaF on DNA damage in rat osteoblast model. NaF was selected to
affect the rat osteoblast model at 1.00, 2.00, and 4.00 mmol/L concentration and measured the effect of sodium fluoride at different exposure concentrations of antioxidant enzyme activity and content of oxidative damage products (ROS), to further observe protein expressions of Nuclear Factor
erythroid 2-Related Factor (Nrf2), Quinone oxidoreductase 1 (NQO1) and HO-1. We established the primary osteoblasts of suckling rats’ models by Sodium fluoride (NaF) to explore the oxidative stress and DNA damage of NaF in the rat osteoblasts. It was found that, the survival rate of
rat osteoblasts decreased as the concentration of NaF was increased after exposal to NaF at certain concentrations, and the antioxidant function was impaired, leading to oxidative stress and DNA damage. Consequently, our results indicated that, the Nrf2/ARE signaling pathway was activated
to initiate the expressions of downstream NQO1 and HO-1 antioxidant reactive proteins. In conclusion, the present study presented the oxidative stress of NaF in the rat osteoblasts at certain concentrations and DNA damage was observed. Nrf2 signaling was partly responsible for the oxidative
stress induced by Sodium fluoride.
Mechanism of cinobufacin anti-metastatic colorectal cancer based on integrated mining of proteomics and bioinformatics
Authors: Dong Yunfei , Bao Huiyan , Fang Zhengyu , Chen Zhi
Published: August 2023

In this study, the effector proteins of anti-metastatic colorectal cancer (CRC) were mined through proteomics based on the preliminary screening of cinobufacin anti-cancer genes using bioinformatics and the role of cinobufacin anti-metastatic CRC was explored. Proteomics was used to
analyze the differential expression of proteins in biopsy tissues of patients with metastatic CRC and bioinformatics was adopted to screen for cinobufacin tumor suppressor genes. The effects of proteomics-mined effector protein CyclinD1 on cell proliferation and colony formation were evaluated
by MTT assay and colony formation assay. The cinobufacin sensitivity of metastatic CRC cells were also assessed. After proteomic analysis, the common targets of “Cinobufacin-metastatic CRC” were screened out, among which the tumor-associated CyclinD1 was listed as its level was
increased in biopsy tissues of patients with metastatic CRC. The preliminary screening of cinobufacin anti-cancer genes based on bioinformatics found that tumor suppressor gene P53 was closely related to metastatic CRC. CyclinD1 had a relation with P53 mRNA. Further studies showed that P53
was the direct target of CyclinD1 and CyclinD1 overexpression enhanced the sensitivity of metastatic CRC cells to cinobufacin. Using bioinformatics-based gene database analysis technology and proteomics mining technology, the differential expression of proteomics in the biopsy tissues of patients
with metastatic CRC and the tumor suppressor genes regulated by cinobufacin were identified. CyclinD1 and P53 found by bioinformatics might be biomarkers for metastatic CRC prognosis, providing a new possible molecular target for anticancer therapy.
Carbon-based solid acids catalyze the synthesis of ethylene glycol monoacetate from ethylene glycol and acetic acid
Authors: Tian Zenan , Liu Jidong , Liu Yida , Li Wensong , Zhang Ridong , Lv Jianhua
Published: August 2023

Activation is essential for improving the structure and surface functional groups of carbon-based solid acids (CSA) during the preparation of carbon materials. This paper compared the catalytic activity of CSA treated non-, KOH-, H3PO4-, and ZnCl2-activation
in esterification of ethylene glycol (EG) and acetic acid (HAc) to synthesize ethylene glycol monoacetate (EGMA). The catalysts were characterized using N2 isothermal adsorption–desorption, acid-base titration, Fourier transform infrared (FT-IR) spectroscopy, scanning electron
microscopy (SEM), Raman spectrometer, X-ray photoelectron spectroscopy (XPS), and X-ray diffractometer analysis (XRD). The results showed that activation treatment clearly enhanced the CSA’s specific surface area and significantly influenced the distribution of surface functional groups.
Notably, the CSA activated with H3PO4 had a markedly higher density of –SO3H groups than the non- activated, KOH- activated, and ZnCl2-activated CSA. The high density of –SO3H on the catalyst surface is advantageous for the
synthesis of EGMA from EG and HAc, making the H3PO4-activated CSA the best performer. The reaction conditions were optimized on H3PO4-activated CSA, resulting in a 74.1% conversion of EG and an 81.8% selectivity of EGMA at 100 °C for 30 min,
and the EG/HAc molar ratio of 1:2. H3PO4-activated CSA was not obviously deactivated even after five cycles, and the decrease of its catalytic performance was related to the leaching of –SO3H on the catalyst surface. Overall, these findings suggest that
H3PO4-activated CSA offers the benefits of less cost, a simpler preparation method, and greater catalytic activity compared to other catalysts, making it a desirable option for the synthesis of EGMA.
Fabrication of co-polymeric nanoformulation loaded with antibiotic ofloxacin and immunosuppressant tacrolimus for pediatric sepsis-induced acute lung injury
Authors: Yuan Shuang , Qian Dai , Su Wanyun
Published: August 2023

Sepsis is a complicated, life-threatening illness caused by pathogen invasion triggered by a dysfunctional immune system in the host. Even though several antimicrobial and immunosuppressive therapies have been suggested, no clinically viable treatments for sepsis exist. This research
aims to find a way to cure sepsis and reduce its symptoms by creating a nanoparticle system that specifically targets inflammation and drug delivery sites. To fabricate the PLGA-NPs loaded with the wide-range antibiotic Ofloxacin (OFX) and the immunosuppressant anti-inflammatory Tacrolimus
(TCR), we opted for the biocompatible and biodegradable PLGA (poly(lactide-co-glycolide acid)). Grafting the γ3 peptides that can selectively conjugate to the intercellular adhesion molecule-1 (ICAM-1), which is abundantly articulated on the inflammatory endothelium cells surface,
confers the targeting capacity of the nanoparticles toward inflammatory areas. Good biocompatibility, lower hemolytic rate, and minimal toxicity are hallmarks of dual drug-loaded γ3@PLGA nanoparticles. This work presents a straightforward and sturdy nanoplatform for treating pediatric
sepsis-induced acute lung injury as a first step toward developing multifunctional nanomedicine for clinical translation.
Platycodin D regulates HAX1 to promote TRAIL apoptosis after interventional liver cancer and induces programmed necrosis in cancer cells
Authors: Mao Yingmin , Hu Zhe , Zeng Yunjian , Shi Zili , Zhou Yuqi
Published: August 2023

Platycodin D (PD) is a tumor suppressor in cancers. However, its role in liver cancer, especially after interventional liver cancer, remains unknown. We detected the expression of HAX-1 after interventional liver cancer by IHC and reverse transcription-polymerase chain reaction, RT-PCR
(RT-PCR), and assessed cell proliferation by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide (MTT). PD was down-regulated in CD44+CD24−/low HCC cells. In addition, the results showed that PD could induce Tumor necrosis factor-associated
apoptosis-inducing ligands (TRAIL) to be sensitive to apoptosis. HCLS1 associated protein X-1 (HAX-1) is directly regulated by PD in liver cancer cells. We demonstrate that PD promotes apoptosis through mitochondrial/ROS pathway. PD increases TNBCSCs sensitivity to apoptosis via targeting
HAX-1 and it might be useful in the treatment of HCC after interventional procedures.
Mechanism of Apatinib gene carried with carbon nanotube in regulating the growth and chemosensitivity of human papilloma virus through inducing AMPK/TSC2/mTOR signal pathway
Authors: Xu Xing , Liang Mengjie
Published: August 2023

This study assesses the mechanism of Apatinib gene carried with CNT in regulating the growth and chemosensitivity of HPV through inducing AMPK/TSC2/mTOR signal pathway. SiHa cells were divided into blank set, empty carrier set, Apatinib set and Apatinib set carried with CNT randomly. Cell cycle of SiHa cells was detected through CCK-8 test, clone formation test and FCM. The mRNA presentation of Cyelin D3 was detected with Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The protein expression of Cyelin D3, presentation and activation of AMPK, TSC2 and mTOR was detected with Western Blot assay. Expression of VEGFR-2 in Apatinib set and Apatinib set carried with Carbon nanotube (CNT) was reduced. The proliferative rate in Apatinib set was lower than in control set notably. The rate of clone formation in Apatinib set carried with CNT was declined notably compared with control set. The cell cycle was restrained in Apatinib set carried with CNT. The IC50 concentration of TAX in Apatinib set carried with CNT was lower than in control set. The expression of p-AMPK in Apatinib set and Apatinib set carried with CNT was elevated compared with control set. The active expression of AMPK was prompted in Apatinib set. And phosphorylation of mTOR was restrained. The growth of HPV was restrained and chemosensitivity of HPV was improved by Apatinib gene carried with CNT through inducing AMPK/TSC2/mTOR signal pathway.
Analysis of the mechanism of liquiritigenin in promoting cardiomyocyte regeneration based on miRNA-34b-TIA-1-SG molecular network-mediated autophagy
Authors: Zheng Jiadi , Zheng Nan , Lin Meise
Published: August 2023

This study aims to clarify that miRNA-34b affects the autophagy through regulating TIA-1-SG and explore the role of miRNA-34b-TIA-1-SG molecular network-mediated autophagy in cardiomyocyte regeneration. 32 patients with autophagy injury caused by myocardial ischemia perfusion admitted
to the hospital were included and 32 healthy controls were enrolled at the same time. ELISA was used to detect miRNA-34b level. Cardiomyocytes were isolated and cultured by adherence method and the relationship between miRNA-34b and TIA-1-SG was analyzed by dual-luciferase gene reporter assay.
The correlation between myocardial autophagy and liquiritigenin treatment was assessed along with qRT-PCR analysis of the changes of genes (TIA-1, SG, ULK1, OsATG7, FAM176A, Beclin 1). miRNA-34b expression in 32 patients with myocardial autophagy injury was higher than normal group. miRNA-34b
binds to the 3′-UTR of TIA-1 and transfection of miRNA-34b inhibitor increased the luciferase activity, which was not affected in pMIR-TIA-1-mut group, confirming the relationship between miRNA-34b and TIA-1. The addition of liquiritigenin can promote the reduction of expression of myocardial
autophagy injury-related genes. In the absence of liquiritigenin, the expression of genes related to myocardial autophagy injury increased significantly. Autophagy imbalance plays an important role in myocardial infarction, which participates in myocardial infarction. miRNA-34b can target
and regulate TIA-1 gene, promote myocardial infarction repair, and inhibit the state of autophagy by regulating autophagy-related gene TIA-1. Liquiritigenin regulates autophagy imbalance in myocardial infarction to delay myocardial remodeling and improve prognosis.