bluuebunny/crossref_metadata_2025 huggingface dataset:
Validating Medical Treatment Decision Makers in the Medical Records of a Teaching Hospital
Authors: Osman Abdi D , Smithies Lisa , Jones Daryl , HOWELL Jocelyn , Braitberg George
Published: November 2022

Background
A Medical Treatment Decision Maker (MTDM) is appointed to make medical treatment decision on behalf of a person who cannot make such decisions for themselves, provided they are reasonably available and willing to make such decisions. In the Emergency Department (ED), the clinicians’ ability to contact MTDMs is an essential for patient care, particularly in time-critical situations or when end of life discussion is necessary. Our primary objective was to review the verification process and assess the accuracy of MTDM contact numbers in the Health Information System (HIS) at our institution.
Methods
Quantitative method with retrospective observational study design with phone interview transcript was used. Data was imported from Microsoft Office Excel Spreadsheet to SPSS™ for analysis and statistical significance indicated by a two-sided P value < 0.05 and Confidence Interval (CI) range.
Results
Of the one hundred and sixty-nine patients selected, seventy six percent had a MTDM listed selected of whom 59% were available for follow up. The process of registering and validating a person as a MTDM in the HIS was found to be compliant with legal requirements. There was no reported, observed or known existing contact update alert process in the HIS after a period of patient record inactivity. Patient advancing age had statistically significant association with the number of call attempts made to reach the listed MTDM (P = 0.043; CI, -3.541 to -0.057) and the MTDM’s consent to participate (p = 0.023). Patient gender had statistically significant association with successfully contacting the listed MTDM (p = 0.036; CI, 0.012 to 0.355) and the number of call attempts made to reach the listed MTDM (P = 0.039; CI, 0.006 to 0.218).
Conclusion
Identification and listing of MTDMs for elderly patients need to improve, as the study found only 76% had MTDMs listed. In our sample only 59% responded to a contact call, a concerning result when considering the possible time critical nature of such a call. Four participants declined to consent as a call originating from a hospital extension is displayed as “No Caller ID” on smart phones. Concern about cybersecurity or data breaches, may impact on the ability to maintain accurate MTDM details
Eastern redcedar roots create legacy effects that suppresses growth of prairie species
Authors: Bennion Leland Dwyth , Ward David
Published: November 2022

The expansion of woody species from their historical ranges into grasslands is a global problem. Understanding the mechanisms that enable species to successfully establish and then re-encroach following their removal is critical to effectively managing problem species. Legacy effects are a mechanism that could be critical to the re-establishment of woody encroachers following their removal. Legacy effects occur when a species alters the biotic and abiotic environment in a way that affects communities that establish subsequently. In this study, we assess whether Juniperus virginiana, a North American woody encroacher, generates legacy effects that affect communities that establish following removal of this species from an experimental grass community. We treated three soil groups with different percentages of J. virginiana roots. We found escalating suppression of overall community biomass and the biomass of each of the individual species with increasing percentages of root treatments. Our results suggest that J. virginiana exudes an allelochemical into soils that inhibits the growth of certain grasses and thus has legacy effects on future occupants. We suggest that the inhibition of the development of grasses in areas where J. virginianahas been removed is a mechanism that favors the re-establishment of J. virginiana. Our results indicate the legacy effects of J. virginianamust be considered when conducting removal and restoration of J. virginiana infested lands.
WITHDRAWN: E-scooter-related orthopedic injuries and the treatments applied. Are these a new means of transportation or a new cause of trauma?
Authors: İğrek Servet , Ulusoy İbrahim
Published: November 2022

Introduction
E-scooters have become increasingly popular in Turkey due to easy accessibility and the widespread use of a rental system, but in parallel with this, the number of e-scooter-related injuries has increased gradually. The aim of this study was to determine the types of e-scooter-related orthopedic injuries, to evaluate hospitalization and surgical treatments, and to investigate the loss of work of patients and the burden incurred by the healthcare system.
Materials and Methods
This retrospective multicentre study included all orthopaedic referrals, who were admitted to two major trauma departments in Diyarbakır from January 2022 to July 2022. Patient data were analyzed, including demographic characteristics, injury pattern, types of injury and subsequent treatment.
Results
In a total of 62 patients, 105 orthopaedic injuries were identified in 62 patients, comprising 72.5% males and 27.5% females, with a median age of 34.21 years. Fifty-six (90.3%) patients were riders, and six were pedestrians. All the e-scooters were rented. There were 44 fractures (41.9 % of the total recorded injuries) including 8 (12.9 %) open fractures. Surgery was required by 32 patients (51.6%) and 35 (56.4%) required hospital admission leading to hospitalization of 3.7 days on average. The average job loss of working patients after injury was determined as 2.4 months. Helmet use was detected in 6.4% of the e-scooter users but no other protective equipment was detected in any of the patients, and 19,3% of the patients had a blood alcohol level of>10 mg/dl.
Conclusions
The injuries that may result from an e-scooter accident can have high mortality, long-term hospitalization and long-term job loss in the community. This imposes a significant financial burden on the national healthcare system and adversely affects public health. There is a need for precautions to be implemented such as infrastructure organisation, increased awareness of motor vehicle drivers and e-scooter drivers, and increased enforcement of rule compliance including not using e scooters after alcohol consumption and the use of protective equipment.
Screening of Atrial Fibrillation in Dental Practices: a qualitative feasibility study
Authors: Hassan Amaar , Moreno-Lopez Rosa , Lane Deirdre , Lip Gregory , Harris Rebecca , Mughal Asfa , Weir Corina
Published: November 2022

Introduction: To increase the detection of unknown atrial fibrillation (AF), general practitioners have started screening their patients using small devices. It is thought that can also be utilised as they have regular access to patients.
Aims: This qualitative study aimed to explore perceptions and barriers of dental staff for screening of AF using a hand-held electronic device in primary dental care.
Setting: A large mixed NHS and private general dental practice in the Northwest of England
Methods: Views from staff included dentists, dental therapists, dental nurses, and managers. Interviews were semi-structured, face-to-face, audio recorded and transcribed verbatim. The interviews continued until there were no new themes or patterns emerging from the data and thematic saturation had been achieved.
Results: Eleven participants were interviewed, and main themes generated were methodology for screening, acceptability for screening within the practice, attitudes to screening, and implementation of screening. Overall, participants were positive about implementing screening in a dental practice but expressed concerns about time and remuneration. Staff also gave encouraging feedback regarding the simplicity of the portable screening device.
Conclusions: Participants felt that AF screening was a good concept but may be challenging to implement in NHS Dentistry, especially due to the pandemic.
Can we easily and accurately predict the occurrence of bone nonunion after posterior lumbar interbody fusion based on the lumbar indentation value and the new muscle health grade?
Authors: He Peifeng , Wang Minglang , Zhou Qingzhong , Lei Fei , Yuan Hao , Chen Zan , Li Songke , He Qiang , Feng Daxiong , Zheng Lipeng
Published: November 2022

Background: The degeneration of lumbar paraspinal muscles has been confirmed to affect bone nonunion after posterior lumbar interbody fusion combined with pedicle screw fixation (PLIF-PSF). However, there is still no convenient and accurate muscle evaluation method to predict bone nonunion after PLIF-PSF. This study aims to provide a rapid and feasible muscle evaluation method for clinical front-line surgeons to predict bone nonunion after PLIF-PSF.
Methods: A total of 236 patients who received PLIF-PSF at L3–L4, L4–L5, and L5-S1 segments and the follow-up for 1 year were analyzed retrospectively. Based on the analysis of the preoperative lumbar magnetic resonance imaging (MRI), the lumbar indentation value (LIV), the cross-sectional area of paralumbar muscles/body mass index (PL–CSA/BMI), and the Goutallier classification were measured at the intervertebral disc of the L4-L5 segment, and the new muscle health grade was performed. According to the bone nonunion of these patients evaluated by lumbar X-ray, they were divided into the bone union group and the bone nonunion group.
Results: In terms of LIV and PL–CSA/BMI, the bone union group was higher than the bone nonunion group at L4-L5 segments (p=0.02, 0.03). In terms of the Goutallier classification, the proportion of patients at grade 3 or 4 in the bone nonunion group was significantly higher than that in the bone union group (P < 0.01, =0.04, and =0.02). In addition, the proportion of patients at a poor muscle health grade in the bone nonunion group was significantly higher than that in the bone union group (P < 0.01, < 0.01, and < 0.01). Through the binary logistic regression, it can be revealed that the new muscle health grade was an independent predictor of bone nonunion after PLIF-PSF (p=0.01, 0.01, and 0.03).
Conclusions: Compared with LIV, PL–CSA/BMI, and Goutallier classification, the new muscle health classification is more effective in predicting bone nonunion, and it is expected to be applied in clinical practice. Moreover, it can be further confirmed that the degeneration of lumbar paraspinal muscles is an important factor affecting bone nonunion.
DWPMIndex: a distributed spatiotemporal data index
Authors: Tang Na , Luo Kaiyuan , Lu Jieyu , Li Jingjing , Tang Yong
Published: November 2022

With the GPS mobile devices were widely used and the rapid development of wireless network communication technology. A large number of Moving-Object data that contains spatiotemporal attributes has appeared in our worldwide. However, it is always a hot research issue about how to efficiently store, manage, query, and analyze these massive but valuable spatiotemporal data.This paper proposes a distributed NoSQL index, DWPMIndex (Distributed Weighted Phase-point Moving Object Index) which is based on the Hadoop platform, a distributed infrastructure developed by Apache. DWPMIndex mainly provides data partitioning technology, global index, and local index. We adopted a data partition algorithm for moving objects based on the sampled road segment data which satisfied "balanced partition" and "local dispersion". Furthermore, we creatively design two indexing data structures, i.e., QMS-tree (Quad-tree based on Median point to Split) and WPM-tree (Weighted Phase-point Moving Object Tree), which accelerates global and local indexing tremendously. DWPMIndex can support plentiful queries efficiently, including Spatio-temporal range query, spatial range query, and trajectory query. Experimental results show the powerful query efficiency and scalability of DWPMIndex.
A Field Study on Thermal Comfort Status of Vernacular Dwellings in Cold Regions of China, Taking Linyi as An Example
Authors: Duan Yuechen , Zhang Tao , Jiao ZhuangQing , Ye Xin , Hu QiNian , Gao Weijun
Published: November 2022

With the deepening of Chinese rural revitalization in recent years, residents' requirements for indoor thermal environment are increasing. It is widely agreed that the indoor thermal environment of vernacular dwellings in cold areas of China is poor in winter, which seriously affects the thermal comfort and health status of residents. Therefore, in light of indoor thermal comfort improvement, this paper first measured the indoor thermal environment of vernacular dwellings in Linyi City, and found that the indoor thermal environment in winter is very bad, and the indoor thermal environment in summer is relatively comfortable. Secondly, by conducting a thermal sensory investigation, we found that the local residents' adaptability to the local cold climate in winter is greater than that to the hot and humid climate in summer. In addition, to identify residents’ comfort range, we established a scatter plot of the relationship between the ambient temperature and the residents' thermal sensory, which show that the residents' comfort range of 23.3℃~30.0℃ in summer and 11.25℃~17.5℃ in winter. This study compares the current indoor thermal environment of vernacular dwellings with the thermal comfort range of residents from the perspective of users, which can be used as a guide and hopefully these results can provide data support for the future indoor thermal comfort improvement in rural vernacular dwellings in Chinese cold rural areas.
Texture analyses of the lateral pterygoid muscle in young patients with temporomandibular disorder based on magnetic resonance imaging: a clinical study
Authors: Luo Dan , Qiu Cheng , Zhou Ruizhi , Shan Tao , Yan Wenjing , Yang Jianjun
Published: November 2022

Objectives
The aim of this study was to investigate the etiology of temporomandibular disorder (TMD) in young patients using clinical and magnetic resonance imaging (MRI) features of the lateral pterygoid muscle (LPM).
Materials and methods
The patients were divided into four groups: healthy control, disc without displacement (DWoD), disc displacement with reduction (DDWR), and disc displacement without reduction (DDWoR) groups. Demographic information and clinical symptoms of patients in each group were recorded. LPM textures on magnetic resonance imaging (MRI) were compared among all groups. LPM fasciculation was examined.
Results
Patient sex or age did not differ significantly among groups, while several clinical symptoms and signs and some LPM texture parameters did. The proportion of type B LPM fascicles was significantly higher in DDWR and DDWoR groups than in healthy control and DWoD groups.
Conclusion
In this study, patients in DDWR and DDWoR groups showed unclear texture, no obvious regularity, and relatively uniform gray distribution on MRI of LPM.
Clinical relevance
This study found that the texture of the LPM in young TMD patients was different from that in normal people, especially in TMD patients with anterior disc displacement. Changes of the LPM may affect the position of the articular disc, thus provides a direction for scholars to study the etiology of TMD.
Needs of early intervention services in community-based integrated mental health care systems in Japan
Authors: Uchino Takashi , Fukui Eriko , Takubo Youji , Iwai Momoko , Katagiri Naoyuki , Tsujino Naohisa , Imamura Haruhiko , Fujii Chiyo , Tanaka Kuniaki , Shimizu Tetsuo , Nemoto Takahiro
Published: November 2022

Purpose: There is a global movement to develop and implement community-based integrated mental health systems. The present study aimed to clarify needs and perceptions of early intervention in those systems among individuals with mental illness and healthy people in Japan.
Methods: A new questionnaire was developed for this internet survey. Data from 500 outpatients with depression and 500 healthy subjects were sampled according to the demographics of the Japanese population and were analyzed descriptively.
Results: Over 90% of healthy subjects and over 70% of patients were unaware of the common age of onset or lifetime prevalence of mental illness. Over 90% of the healthy subjects and about 70% of the patients could not describe any services where they would feel comfortable discussing mental health problems. In both groups, “adolescents and young adults” were ranked first as a target population for mental health and illness policies. The top requirements for the integrated care systems were mainly related to early intervention, such as promotion and awareness of correct knowledge of mental illness, preventive support for mental health problems before the onset of mental illness, and support for people in the early years after illness onset.
Conclusion: The dissemination of correct knowledge and awareness-raising actions for society and the implementation of specific services targeting the early stage of mental illness are societal requirements. These services need to be considered as components in the integrated mental health care systems that are currently being established in Japan.
DDoS Attack Detection and Classification Using Hybrid Model for Multi-controller SDN
Authors: Gebremeskel Tewelde Gebremedhin , Gemeda Ketema Adere , T Gopi Krishna , Perumalla Janaki Ramulu
Published: November 2022

Software-Defined Network (SDN) brings a lot of advantages to the world of networking through its flexibility and centralized management; however this centralized control makes it susceptible to different types of attacks. Distributed Denial of Service (DDoS) is one of the most dangerous attacks which can frequently launch DDoS attacks towards the controller in order to make it out of service. This work takes the special ability of SDN to propose a solution that an implementation running at the multi-controller to detect DDoS attack at the early stage. The method not only detect the attacks but also identify the attacking paths and start a mitigation process to provide protection for the network devices the moment an attack is detected. This method is based on the entropy variation of the destination host targeted with its IP address and can detect the attack within the first 250 packets of malicious traffic attacking a particular host. Then, fine-grained packet-based detection is performed using deep learning model to classify the attack into different type of attack categories. Lastly, the controller sends the updated traffic information to neighbor controllers. To avoid a single point of controller failure, a multi-controller which is a logically centralized and physically distributed controller has used. The issues related to lack of detailed attack description have been addressed using categorical classification which is allowed to make specific attack descriptions by considering the traffic coming to the controller. The chi-square (x2) test feature selection algorithm has also employed to reveal the most relevant features that scored the highest in the provided data set. The experiment result demonstrate that the proposed Long Short-Term Memory (LSTM) model achieved an accuracy of up to 99.42% using the data set CIC-DDoS2019 which has the potential to detect and classify the DDoS attack traffic effectively in the multi-controller SDN environment. In this regard, it has enhanced accuracy level to 0.42% when we compared with RNN-AE model with data set CIC-DDoS2019, while it has improved up to 0.44% in comparison with CNN model with different data set ICICDDoS2017.
Marital status is an independent prognostic factor for Intrahepatic cholangiocarcinoma patients: An analysis of the Surveillance, Epidemiology, and End Results (SEER) database
Authors: Yang Yong-jing , Diao Jian-dong , Xu De-quan , Wang Xue , Wu Chun-jiao
Published: November 2022

Objectives
The effect of marital status on the prognosis of intrahepatic cholangiocarcinoma (ICC) has not been strictly studied. Therefore, the present study focused on exploring whether marital status was related to ICC prognosis.
Methods
This study chose cases from Surveillance, Epidemiology, and End Results (SEER) database during the 2004–2015 period. Cancer-specific survival (CSS) and overall survival (OS) were compared between married and unmarried groups.
Results
We enrolled 4550 eligible cases, among them, 2760 were married (60.66%). Married subjects had more younger age (< 65 years), male sex, white race, tumor size ≥ 5cm, grade I/II, and along with higher proportions of underwent surgery, chemotherapy and radiotherapy (all P < 0.05). Married cases had the CSS rates of 14.29% and 9.06% at 3 and 5 years, while those for unmarried cases were 12.06% and 8.14%, separately (P < 0.0001). In the meantime, married cases had the OS rates of 13.30% and 8.06% at 3 and 5 years, whereas those for unmarried cases were 10.72% and 7.92%, separately (P < 0.0001). Upon multivariate regression, marital status was identified to be the factor that independently predicted patient prognosis, and married cases had superior CSS [hazard ratio (HR):0.903; 95% confidence interval (CI): 0.844–0.966; P = 0.003] as well as OS (HR: 0.903; 95%CI: 0.845–0.965; P = 0.003). Upon subgroup analysis, married cases had prolonged CSS as well as OS to unmarried cases of almost every subgroup, especially for patients who have not received surgery, chemotherapy, or radiotherapy.
Conclusion
This study identified marital status as the factor independently predicted ICC prognosis. The dismal prognostic outcome of unmarried cases may attribute to inadequate treatments, socioeconomic and psychosocial factors.
Ferroptosis-related ACSL3 and ACTC1 predict metastasis free survival for prostate cancer patients unfergoing radical radiotherapy
Authors: Shi Xu , Feng Dechao , Zhu Weizhen , Han Ping , Yang Lu , Wei Qiang , Wei Wuran
Published: November 2022

BACKGROUND
To develop a ferroptosis-related gene prognostic index (FGPI) to predict biochemical recurrence and metastasis for prostate cancer (PCa) patients undergoing radical radiotherapy.
METHODS
All analyses were conducted with R version 3.6.3 and its suitable packages.
RESULTS
ACSL3 and ACTC1 were used to establish the FGPI score. Patients in high-risk group had higher risk of BCR (HR: 2.52, 95%CI: 1.49–4.26; p = 0.001) and metastasis (HR: 4.89, 95%CI: 2.12–11.29; p = 0.001) than those in low-risk group, and these results were consistent with external validation of TCGA database (BCR: HR:1.74, 95%CI: 1.04–2.91; MFS: HR: 2.23, 95%CI: 1.44–3.44). Moreover, the diagnostic accuracy of FGPI was also confirmed. Patients in higher expression of lncRNA PART1 had significantly lower risk of metastasis compared to their counterpart (HR: 0.27, 95%CI: 0.10–0.72). Gene set enrichment analysis showed that high-risk patients were negatively associated with integrin signaling pathway, laminin interactions, and fatty acid biosynthesis. PHA-793887 might be sensitive to ACSL3 and ACTC1, and the possible cell lines used to investigate were PC3 and DU145.
CONCLUSIONS
We found that FGPI based on ACSL3 and ACTC1 might be used to predict BCR and metastasis probability for PCa patients.
Incorporating tree diversity for a better understanding of urban form-air quality relationships through mobile monitoring
Authors: Tian Ye , Wang An , Mora Simone , deSouza Priyanka , Yao Xiaobai , Duarte Fabio , Lin Hui , Ratti Carlo
Published: November 2022

Context: Air pollution is a major threat to landscape ecology and public health. The conventional LUR (Land Use Regression) method has been developed mostly based on 2-D urban form and emission source information. Besides, the effects of tree diversity on air quality have not been adequately addressed.
Objectives: This study explores the integration of 2-D and 3-D urban form metrics and examines the impact of tree diversity in urban form-air quality relationships.
Methods: We introduce these predictors into the LUR tools using unique NO2 datasets collected through opportunistic mobile monitoring in the Bronx, New York, and Oakland, California, and further apply lacunarity to investigate the spatial scale sensitivity for regression predictors.
Results: The lacunarity-optimized model helps to reduce the computation burden by finding the upper limit of the spatial heterogeneity of predictors while keeping the model accuracy in both Bronx (R2≈0.50) and Oakland (R2≈0.79). Furthermore, although deciduous trees are surrounded by the highest NO2 concentrations (9.73 ppb and 3.61 ppb in the Bronx and Oakland, respectively), the increase in tree diversity could facilitate the reduction of NO2 concentration. However, according to the non-monotonic and marginal effects of tree diversity on NO2 concentration, a higher level of tree diversity is not always better.
Conclusions: It is reasonable to seek a balance between the diversity and dominance of tree species to effectively improve air quality on the city scale. The findings are useful for both environmental scientists striving for better air quality and urban planners that care for the well-being of cities.
A Benchmark of Pre-processing Effect on Single Cell RNA Sequencing Integration Methods
Authors: Anaissi Ali , Zandavi Seid Miad , Suleiman Basem , Alyassine Widad , Braytee Ali , Vafaee Fatemeh
Published: November 2022

Single-cell data analysis can transform the practice of personalised medicine by facilitating characterisation of disease-associated molecular changes across every single cell. Advanced single-cell multimodal assays can now simultaneously measure different types of molecules (e.g., DNA, RNA, Protein) across hundreds of thousands of individual cells to provide a complete molecular readout of the state of the cell. A key analytical challenge is to integrate single-cell measurements across different modalities. In recent years, different methods have been developed to integrate different molecular modalities of single cell measurements, yet there is no systematic evaluation on the performance of different techniques with respect to different pre-processig strategies. Here, we consider a general pipeline of single-cell data analyses comprising normalisation and data integration followed by the most popular dimensionality reduction methods where several algorithms can be adopted to each module in the pipeline. The performance of different algorithm combinations often depends on the sizes and characteristics of datasets. This paper explores the optimal framework of different algorithms using six datasets across diverse modalities, tissues and organisms. We use three evaluation metrics (Silhouette Coefficient Score, Adjusted Rand Index and Calinski-Harabasz Index) to explore the model's overall performance from the two aspects, including clustering performance and time efficiency. We conduct experiments based on combinations of seven normalization methods, four dimensional reduction methods, and five integration methods. Our results demonstrate that for the data integration module, the clustering performance of Seurat and Harmony are more prominent, but the time efficiency of Harmony was better. At the same time, the performance of Seurat for small data sets is superior. For the dimensionality reduction module, the UMAP method shows promising results in compatibility with the integration methods. The data normalization method could be differentiated according to different integration methods.
A Machine Learning based Resource Efficient Task Scheduler for Heterogeneous Computer Systems
Authors: Hayat Asad , Khalid Yasir Noman , Rathore Muhammad Siraj , Nadir Muhammad Nadeem
Published: November 2022

Heterogeneous computer systems are becoming mainstream due to disparate processing and performance capabilities of multi-core architectures. It consists of different type of devices, i.e., Central Processing Units (CPUs), accelerators, and Graphics Processing Units (GPUs). In the heterogeneous computing environment, if one device is more powerful in terms of computing capability, the scheduling schemes generally favor the powerful device, and that device becomes overloaded while the other device is underutilized. This load imbalance problem results in increased execution time. In this research, we propose load-balanced task scheduler combined with machine learning based device predictor. The device predictor is used to predict execution time both on CPU and GPU devices, and a device with shorter predicted execution time is considered as a suitable device for that particular task. However, it may happen that a high fraction of tasks map only on one type of device since that device is a suitable device for them. Such situation leads to the problem of load imbalance. We use work stealing based task scheduler as part of our solution that allows an idle device to process tasks from the queue of another’s device. In this way we can avoid load imbalance, minimize the overall execution time of tasks, and maximize the device utilization and throughput. We evaluate the performance of our proposed solution into two stages. Firstly, we measure the error rate of our machine learning predictor using three different algorithms (i.e., random forest, gradient boosting, and multiple linear regression). We demonstrate that random forest performs better with marginal error rate. Secondly, we compare the performance of work stealing task scheduler with other scheduling alternatives. Our results show that the proposed solution reduces execution time by 65.63%, increased resource utilization by 93.3%, and throughput by 65.5% in comparison to baseline scheduling schemes.
TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression
Authors: Wang Haodong , Cui Huaiping , Yang Xinjun , Peng Lipan
Published: November 2022

Background: The lack of obvious symptoms of early GC as well as the absence of sensitive and specific biomarkers results in poor clinical outcomes. High TUBA1C expression has been shown to be closely related to a poor prognosis in in various cancers, however, the role of TUBA1C in GC have not yet been elucidated. This study, for the first time, revealed that TUBA1C drives the growth of GC cell lines both in vitro and in vivo.
Methods: The expression of lncRNA EGFR-AS1 was detected in human GC cell lines by qRT–PCR. MS experiments following RNA pulldown assays found that EGFR-AS1 directly binds to TUBA1C, the CCK8, EdU, transwell, wound-healing, cell cycle assays and animal experiments were conducted to investigate the function of TUBA1C in GC. Combined with bioinformatics analyses, reveal interaction between E2F1, Ki-67, PCNA and TUBA1C by western blot. Rescue experiments furtherly demonstrated the relationship of EGFR-AS1and TUBA1C.
Results: TUBA1C was proved to be a direct target of EGFR-AS1, TUBA1C promotes gastric cancer proliferation, migration and invasion by accelerating the progression of the cell cycle from the G1 phase to the S phase and activating the expression of tumor suppressor genes: E2F1, Ki-67, and PCNA.
Conclusions: TUBA1C is a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression and could be a novel biomarker and therapeutic target for GC.
Cobalt and Calcium-based Metal-Organic Frameworks (MOFs) as an Energy and Advanced Electrode material for Supercapacitors
Authors: BM Omkaramurthy , G Krishnamurthy , Foro Sabine
Published: November 2022

Cobalt and Calcium - based metal-organic frameworks (Co-MOF and Ca-MOF) [Co (tpa) (mi) and Ca (tpa)] (tpa = terephthalic acid and mi = methyl imidazole) was synthesized through solvothermal method. Structural characterization revealed that metal centers are equally occupied by Co2+ and Ca2+ ion. Supercapacitive behaviour of synthesized Co-MOF and Ca-MOF were evaluated using cyclic voltammetry (CV), galvano static charge/discharge, and electrochemical impedance spectroscopy (EIS) measurements in 6 M KOH as electrolyte. The Co and Ca-MOFs exhibited outstanding specific capacitance of 1726 and 185Fg− 1 at a discharge current density of 1 Ag− 1, good rate capability, and 97.4% capacitance retention after 3000 cycles. To the best of our knowledge, Co-MOF and Ca-MOFs are used in supercapacitor and its encouraging properties indicate that Co/Ca-MOF can be promising candidate as an advanced electrode material for supercapacitors.
Contributions of vascular burden and amyloid abnormality to cognitive decline in memory clinic patients
Authors: Gils Veerle van , Ramakers Inez , Jansen Willemijn J. , Banning Leonie , Kučikienė Domantė , Costa Ana Sofia , Schulz Jörg Bernhard , Visser Pieter Jelle , Verhey Frans , Reetz Kathrin , Vos Stephanie J.B.
Published: November 2022

Background.
Alzheimer’s disease (AD) pathology and vascular burden are highly prevalent and often co-occur in elderly, but it remains unclear how both are associated with cognitive decline. We aimed to investigate whether amyloid abnormality and vascular burden synergistically contribute to cognitive decline in a memory clinic population.
Methods.
In this longitudinal study, 228 participants across the clinical AD spectrum (SCD, MCI, dementia) were included from Maastricht and Aachen university memory clinics. Amyloid abnormality (A) was measured by cerebrospinal fluid (CSF) Aβ-42, using data-driven cut-offs. Vascular burden (V) was defined as having either moderate to severe white matter lesions (Fazekas score ≥ 2), one or more microbleeds, intracranial hemorrhage or infarcts on MRI. Participants were divided into four groups: A-V-, A-V+, A + V-, and A + V+. Outcome measures were global cognition, memory, processing speed, executive functioning, and language z-scores. Linear mixed models were used, adjusted for age, sex, education, with center as random factor. Effects of individual MRI vascular measures and Aβ-42 abnormality on cognitive decline were also assessed. In secondary analyses, presence of vascular risk factors and disease were used as V definitions.
Results.
At baseline, the A + V + group scored worse on global cognition and language compared to all other groups. The A + V + group also had worse memory scores compared to A-V + and A-V- groups. Over time, with an average follow-up of 2.7 years (SD = 1.5), the A + V + and A + V- groups showed faster decline in global cognition than the A-V + and A-V- groups. Only the A + V- group showed decline on memory and language scores. The A-V + group did not differ from the A-V- group in any domain. Findings were similar in persons without dementia and when vascular risk factors and disease were used as V definitions. When assessing MRI vascular measures individually, no synergistic effects on cognitive decline were shown and only microbleeds were independently associated with executive functioning decline over time.
Conclusions.
Our study shows that amyloid abnormality predicts cognitive decline in a memory clinic population independent from vascular burden. Vascular burden has a minor contribution to cognitive decline in these patients. This has implications for prognostic procedures in memory clinic patients.
Nitrogen release and microbial community characteristics during the decomposition of field-incorporated corn straw in Northeast China
Authors: Yan Chao , Yang Daocheng , Xue Yinghao , Song Qiulai , Hou Zhaofang , Yan Shuangshuang , Lyu Xiaochen , Ma Chunmei , Xu Zhiyu , Gong Zhengping
Published: November 2022

Straw incorporation increases the amount of nutrients in soil and has a significant impact on soil nitrogen and microbial communities. However, no study has quantified the amount of nitrogen released and taken up by straw during its decomposition after it is incorporated into soil in the field. In this study, a method for estimating the amounts of nitrogen released and taken up by field-incorporated straw was proposed based on 15N-labeled corn straw, and the relationships between nitrogen release and uptake amounts and the related microbes were established. The results showed that the amounts of nitrogen released by the straw at different incorporation depths were not significantly different. Among all the treatments, the straw placed 15 cm above the soil surface and the straw mulched on the soil surface had the highest nitrogen uptake, and the straw placed 15 cm above the soil surface had the lowest net nitrogen release. The relative abundances of Burkholderiales, Flavobacteriales, Pseudomonadales, and Sphingobacteriales were significantly positively correlated with the 15N abundance and the nitrogen release speed of the straw. The relative abundances of Rhizobiales and Sphingomonadales were significantly negatively correlated with the 15N abundance in straw and significantly positively correlated with the amount of straw nitrogen uptake.
Optical Frequency Comb Generator Employing Serially Connected LiNbO3 Mach-Zehnder Modulator
Authors: Verma Priyanka , Singh Sukhbir
Published: November 2022

An optical frequency comb having ~ 40 equally spaced frequency lines with 6 dB power deviation is proposed using serially connected LiNbO3 Mach-Zehnder modulator. Semiconductor optical amplifier is employed to get equally spaced and flat optical spectrum. The broadness of ultrashort pulse is enhanced by variation in various factors such as confinement factor and differential gain of SOA. The periodicity of input electrical and central wavelength of optical signal is controlled by tuning frequency and line spacing of equally spaced lines of comb.
Establishment of a new predictive model for the recurrence of upper urinary tract stones
Authors: Xia Kaiguo , Xu Yuexian , Qi Qiao , Huang Qingfeng , Yao Rui , Hao Zongyao
Published: November 2022

The purpose of this study is to construct a new prediction model to evaluate the recurrence risk of upper urinary tract stones in patients. We retrospectively reviewed the clinical data of 657 patients with upper urinary tract stones and divided them into stone recurrence group and non-recurrence group. Blood routine, urine routine, biochemical and urological CT examinations were searched from the electronic medical record, relevant clinical data were collected, including age, BMI, stones number and location, hyperglycemia, hypertension, and relevant blood and urine parameters. Then, independent sample t-test, Wilcoxon rank sum test, and Chi-square test were used to preliminarily analyze the data of two groups, and then LASSO and Logistic regression analysis were used to find out the significant difference indicators. Finally, R software was used to draw a nomogram to construct the model, and ROC curve was drawn to evaluate the sensitivity and specificity. The results showed that multiple stones (OR:1.832,95%CI:1.240–2.706), bilateral stones (OR:1.779,95%CI: 1.226–2.582), kidney stones (OR: 3.268, 95% CI: 1.638–6.518) and kidney ureteral stone (OR: 3.375, 95% CI:1.649–6.906) were high risks factors. And the stone recurrence risk was positively correlated with creatinine (OR:1.012,95%CI:1.006–1.018), urine pH (OR:1.967, 95%CI:1.343–2.883), Apo B (OR:4.189, 95%CI:1.985–8.841) and negatively correlated with serum phosphorus (OR:0.282, 95%CI:0.109–0.728). In addition, the sensitivity and specificity of the prediction model were 73.08% and 61.25%, diagnosis values were greater than any single variable. It means the model can effectively evaluate the recurrence risk of upper urinary stones, especially suitable for stone postoperative patients, to help reduce the possibility of postoperative stone recurrence.
Association between Available Blastocyst Formation Rate and Pregnancy Outcome Following Fresh Embryo Transfer
Authors: Wang Longmei , Qiu Pingping , Jiang Lizhi , Jiang Yufei , Li Ping
Published: November 2022

Background: Embryo quality usually has been regarded as a key predictor of successful implantation and pregnancy potential. The identification of embryos that have the capacity to implant and result in a healthy pregnancy is a crucial part of in vitro fertilization (IVF). Usually, the morphologically high quality embryos are chosen for embryo transfer in IVF treatment. The aim of this study was to assess the association between available blastocyst formation rate with pregnancy outcome following first fresh embryo transfer cycles. Our objective was to predict pregnancy outcome according to embryonic development potential, and provide a systematical individual treatment to adjust endometrial receptivity for the next transfer cycle.
Methods: This retrospective, single-center study including 512 fresh embryo transfers conducted between 11/2019–08/2021, which consisted of 385 cleavage-stage (day 3) and 127 blastocyst-stage (day 5) transfers. The two groups were divided into clinical pregnancy group and non-clinical pregnancy group respectively for comparison. The association with available blastocyst formation rate and the clinical pregnancy rate between day 3 or day 5 transfer groups were concerned.
Results: In the day 3 group, there were 275 clinical pregnancies, and the clinical pregnancy rate was 71.43%. Although the 2PN oocytes rate and available embryo rate at day 3 were significantly higher in clinical pregnancy group compared with non-clinical pregnancy group (P < 0.05), the blastocyst formation rate and the available blastocyst formation rate had no significant differences between the clinical pregnancy group and non-clinical pregnancy group (P > 0.05).
In the day 5 group, there were 81 clinical pregnancies, and the clinical pregnancy rate was 63.78%. All the baseline characteristics had no obvious differences between the clinical pregnancy group and non-clinical pregnancy group (P > 0.05). The blastocyst formation rate in the non-clinical pregnancy group was higher than that in the clinical pregnancy group, but the difference was not statistically significant (81.06% vs. 77.03%, P = 0.083). Interestingly, the available blastocyst formation rate was significantly higher than the clinical pregnancy group (66.19% vs. 60.79%, P = 0.014).
Conclusions: In fresh cycles, available blastocyst formation rate was not associated with pregnancy outcome with a day 3 embryo transfer. However, available blastocyst formation rate was negatively associated with pregnancy outcome with a day 5 embryo transfer.
A complicated aortic graft infection case after Bentall’s procedure
Authors: Zhang HongYu , Ke Yingjie , Chen Jiansong , Sun Tucheng , Yu Changjiang
Published: November 2022

Background: The aortic graft infection (AGI) is fatal and has rare evidences of treatment. Here, we report a complicated AGI case after Bentall’s procedure.
Case presentation: A 54-year-old male with a history of Bentall’s procedure, ruptured right sinus aneurysm, ventricular septal defect(VSD) repair and right ventricular outflow tract stenosis(RVOTS) repair was admitted in our hospital with the chief complaint of poor heeling of incision, chilling and fever. Transthoracic echocardiography(TTE), contrast enhanced computed tomography(CECT) found a perigraft leakage pseudoaneurysm. After conservative treatment, the patient was underwent re-operation due to the growing giant pseudoaneurysm. After 8 weeks antibiotic therapy, the patient discharged with no complications.
Conclusions: The treatment of AGI is comprehensive. Successful management requires a prompt diagnosis,sensitive and long-term postoperative antimicrobial therapy, and early surgery depending on the patients’ status and clinician’s experience.
WITHDRAWN: Rapid quantitative analysis of calcium in infant formula powder assisted by long short-term memory with variable importance using laser-induced breakdown spectroscopy
Authors: Ding Yu , Yang Lin-yu , Chen Wen-jie , Chen Jing , Zhao Xing-qiang , Luo Yong
Published: November 2022

Calcium is the main mineral responsible for healthy bone growth in infants. In this study, LIBS was combined with a variable importance-based long short-term memory (VI-LSTM) for the quantitative analysis of calcium in infant formula powder. Firstly, the full spectra were used to establish PLS and LSTM models. The R2 and root-mean-square error (RMSE) of the test set (R2P and RMSEP) were 0.1460 and 0.0093 in the PLS method, respectively, and 0.1454 and 0.0091 in the LSTM model, respectively. To improve the quantitative performance, variable selection based on variable importance was introduced to evaluate the contribution of input variables. The variable importance-based PLS (VI-PLS) model had R2P and RMSEP of 0.1454 and 0.0091, respectively, whereas the VI-LSTM model had R2P and RMSEP of 0.9845 and 0.0037, respectively. Compared with the LSTM model, the number of input variables in the VI-LSTM model was reduced to 276, R2P was improved by 114.63%, and RMSEP was reduced by 46.38%. The mean relative error of the VI-LSTM model was 3.33%. This study confirms the predictive ability of the VI-LSTM model for calcium element in infant formula powder. Thus, combining VI-LSTM modeling and LIBS has great potential for the quantitative elemental analysis of dairy products.
Variations in wood traits and specific gravity in male clone of Populus deltoides Bartr. Ex Marsh
Authors: Aziz Massarat
Published: November 2022

The present study deals with the variations of wood anatomical characters and specific gravity in male F 1 offspring of Populus deltoides. ANOVA indicated that within-ramet difference between the means at differents heights of male offspring clone were significant for specific gravity only and non-significant for other wood traits studied. The specific gravity also increases from base to top of the ramet following an increasing trend. The variations for fiber length and vessel element length were significant for direction. The fiber length, fiber wall thickness and vessel element length significantly varied for different radial position. The interactions of height*direction and direction*position were non-significant for all wood traits except fiber outer diameter in height*direction interaction. The non-significant variations in different wood elements of single ramet of Populus deltoides due to peripheral and radial-direction may be related to the early maturity of clone raised tree. It seems non-significant variations in the wood element dimension’s at different directions of Populus deltoides regulate the homogenous wood properties in the discs. The different types of ratios such as Runkel Ratio, shape factor, fiber length/diameter ratio and wall ratio were determined and are important particularly for determining the suitability of a particular material for pulping and paper making. The objectives of the study is to evaluate the wood quality on the basis of specific gravity, Vessel and fiber morphology and other wood anatomical parameters in the F1 male clone of Populus deltoides.
Interpolation numerical integration method for extremely high-accuracy calculation of ordinary differential equations
Authors: Fukuchi Tsugio
Published: November 2022

There are three types of problems that arise in the numerical analysis of differential equations: (i) the initial value problem (IVP), (ii) the boundary value problem (BVP), and (iii) IVP + BVP. In general, it is natural that (i) and (ii) are thoroughly examined before the study of (iii). Thus far, it has been shown that the BVP of one-dimensional (1D) Poisson equations can be solved with extremely high accuracy and speed using the interpolation finite difference method (IFDM). Furthermore, it has been found that a high-accuracy numerical calculation system for the IVP can be constructed by applying the polynomial interpolation method, as in the case of the BVP of the 1D Poisson equation. This method is called the interpolation numerical integration method (INIM). Numerical integration is possible with an arbitrary accuracy order by specifying the degree of the interpolation polynomial for representing the integrand. The proposed calculation method enables numerical calculations that seem to have no error. IVPs are usually calculated by the fourth-order accuracy Runge‒Kutta method, which is often sufficient for engineering purposes. However, in this paper, we report that a numerical algorithm that agrees with the exact solution up to 15 significant digits has theoretical value.