bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengembangan Organisasi Berbasis Kemaritiman Melalui Bidang Seni Rupa (Studi Kasus Rintara Jaya)
Authors: Hazmi Fariz Al , Ahyari Febri
Published: June 2023

Seni merupakan bentuk kreativitas masyarakat yang memiliki beragam fungsi seperti rekreasi, edukasi maupun sosial. Rintara Jaya merupakan kelompok organisasi yang bergerak di bidang maritim dan pemberdayaan masyarakat dengan konsep kesenian di dalamnya, khususnya seni rupa. Dalam konsepnya, Rintara Jaya menggunakan ilmu seni rupa sebagai program yang dijalankan dalam kegiatan pengabdian masyarakat. Untuk dapat melakukan meningkatkan perkembangan organisasi melalui kesenian, analisis SWOT dapat digunakan sebagai analisis dalam menentukan faktor internal dan eksternal. Metode yang digunakan dalam penelitian ini yaitu metode kualitatif dengan pendekatan studi kasus. Teknik pengumpulan data dilakukan dengan wawancara, observasi dan survey. Hasil analisis SWOT menunjukkan bahwa strategi yang dapat digunakan oleh Rintara Jaya yaitu strategi generik jenis ekspansi (ekspansion) dan variasi strategi yaitu diversifikasi konsentrik, penetrasi pasar dan pengembangan produk. Meskipun saat ini Rintara Jaya memiliki posisi yang baik sebagai organisasi, berbagai kegiatan seni yang telah dilakukan perlu dijadwalkan secara rutin mengingat program pemberdayaan membutuhkan pendampingan terus menerus terutama di wilayah pulau. Kemudian, konten yang menarik telah dilakukan Rintara Jaya membantu dalam membangun jaringan melalui media sosial dengan menyajikan visual-visual dan informasi seputar kemaritiman
Low-cost, high-resolution and no-manning distributed sensing system for the continuous monitoring of fruit growth in precision farming
Authors: Peppi Lorenzo Mistral , Zauli Matteo , Manfrini Luigi , Corelli Grappadelli Luca , De Marchi Luca , Traverso Pier Andrea
Published: June 2023

Accurate, continuous and reliable data gathering and recording about crop growth and state of health, by means of a network of autonomous sensor nodes that require minimal management by the farmer will be essential in future Precision Agriculture.
In this paper, a low-cost multi-channel sensor-node architecture is proposed for the distributed monitoring of fruit growth throughout the entire ripening season. The prototype presented is equipped with five independent sensing elements that can be attached each to a sample fruit at the beginning of the season and are capable of estimating the fruit diameter from the first formation up to the harvest. The sensor-node is provided with a LoRa transceiver for wireless communication with the decision making central, is energetically autonomous thanks to a dedicated energy harvester and an accurate design of power consumption, and each measuring channel provides sub-mm 9.0-ENOB effective resolution with a full-scale range of 12 cm. The accurate calibration procedure of the sensor-node and its elements is described in the paper, which allows for the compensation of temperature dispersion, noise and non-linearities. The prototype was tested on field in real application, in the framework of the research activity for next-generation Precision Farming performed at the experimental farm of the Department of Agricultural and Food Science of the University of Bologna, Cadriano, Italy.
Investigation of Measurement Invariance of Turkish Subtest on ABIDE 2016 in Relation to Characteristics of Teachers: Sub-sampling Method
Authors: ÜLKÜ Süleyman , ATAR Burcu
Published: June 2023

The Ministry of National Education carried out the ABIDE (Monitoring and Evaluation of Academic Skills) in 2016 in order to test the knowledge and skills of 8th-grade students. Since the ABIDE 2016 study was implemented for the first time in our country, it is very important to prove measurement invariance for the validity of the results. Within the scope of this research, the measurement invariance of the success of the students in the Turkish test according to the education level and professional experience of the teachers was examined. In the research, data were obtained from the Ministry of National Education, Directorate-General of Measurement, Evaluation, and Examination Services. Responses of students to the multiple-choice items in the ABIDE 2016 Turkish test and teacher questionnaire data were used in the study. All the data were used in the investigation of measurement invariance according to professional experience. Investigation of measurement invariance according to education level was carried out both by using and not using the method of sub-sampling. Factor 10 and Mplus 7 programs were used in the analysis of the data. At the end of the study, the Turkish achievement model provided all levels of measurement invariance among the student groups formed according to the professional experience and education level of the teachers.
The Discourse of Corruption in Mass Media: Polarization, Ideology, and Challenges
Authors: Sujoko Anang , Wahyudi Dicky , Abdillah Muzakky
Published: June 2023

This article presents the opposing point of view between Tempo Newspaper and Media Indonesia in reporting on the revision of the KPK Law after the presidential inauguration. The analysis focuses on the ideology reconstruction of both of those media. The method uses critical discourse analysis to uncover the invisible power, interests, and ideology behind the contents and elements of the articles and the production of a text on Tempo Newspaper and Media Indonesia. The ﬁndings that Media Indonesia's ideology in reporting the revision of the KPK Law is based on the political interests of the media owners, thus in this case, Media Indonesia considers the revision of the KPK Law as an effort to improve and strengthen the performance of the KPK with a pro-government narrative. Meanwhile, Tempo's ideology in reporting the revision of the KPK Law was based on elite interests, so the ideology used was investigative ideology by building a criticizing narrative based on the facts found. This study aims to increase public awareness of the importance of critically viewing this polarization; thus, the harmful excesses of political propaganda do not easily provoke them through mass media.
PART II: Whale Songs of Wars Not Yet Waged: The Demise of Natural-Born Killers through Human-Machine Teamings Yet to Come
Authors: Zweibelson Ben
Published: June 2023

Current human-machine dynamics in security affairs positions the human operator in the loop with artificial intelligence to conduct decisions and actions. As technological advancements in AI cognition, speed, and weapon sophistication increase, human operators are increasingly being shifted to an on the loop where AI takes more responsibility in warfare and defense decisions, whether tactical or even strategic. Human operators are also falling off the loop, trailing enhanced AI systems as the biological and physical limits because humans are not the same for artificial intelligence in narrow applications. Those likely will expand toward general AI in the coming decades, presenting significant strategic, organizational, and even existential concerns. Further, how natural humans respond and engage with increasingly advanced, even superintelligent AI as well as a singularity event will feature disruptive, transformative impacts on security affairs and even at a philosophical level discerning what war is.
Evaluation of the Effectiveness of Lean Six Sigma Approach for SARS-CoV-2 RT-PCR Turnaround Time (TAT) Improvement at a Hospital-Based Tertiary Laboratory
Authors: Lagamayo Dian , Agbay Rose Lou Marie , Datay-Lim Sarah Jane
Published: June 2023

Objectives. This study aims to evaluate the effectiveness of the Lean Six Sigma approach in improving procedure for (TAT) of reverse transcriptase polymerase chain reaction (RT-PCR) for SARS-CoV-2 testing at The Medical City. Specific objectives of the study are to determine the following: 1) baseline sigma and average TAT (in hours); 2) post-implementation sigma and average TAT (in hours) 3) compare if there is a significant improvement between baseline and post-implementation sigma and average TAT (in hours) 4) effect on workflow efficiency.
Methodology. Lean Six Sigma method for quality improvement was applied using DMAIC: Define, Measure, Improve, and Control. The root causes identified were lack of manpower, equipment, space, and manual and complex processes. Then, process wastes were identified, and corresponding proposed solutions were sustained in the control phase, such as standardization and the use of automation.  Measurement of turn-around time and six sigma of the process were performed for evaluation.
Results. Results showed a significant improvement in the TAT in RT-PCR results, with most results released within 24 hours. The pre-Lean Six Sigma data on TAT were as follows: 24.88% released within 24 hours; 65.14% released within 24-48 hours; 3.56% released within 48-72 hours, and 6.42% released in more than 72 hours. The post Lean Six Sigma TAT were as follows: 95.32% released within 24 hours; 4.29% released within 24 to 48 hours; 0.13% released within 48-72 hours, and 0.12% released more than 72 hours. The computed sigma post-implementation was increased from 3.56 to 4.82. The p-value was calculated using the chi-square test, and the computed chi-square statistic is 1894.1021. The p-value is <0.00001 and the result is significant at p<.05. Although there is a significant decrease in the volume of samples post implementation due to the changing COVID19 situation, real time TAT was improved. It also resulted to increased workflow efficiency with the use of lesser manpower with more appropriate utilization.
Conclusion. Applying the Lean Six Sigma method to improve quality processes in the laboratory is shown to be practical, cost-effective, and straightforward.
Profiling of Genetic Mutations among Adult Filipino Patients Diagnosed with Acute Myeloid Leukemia using Fluorescence In Situ Hybridization from 2014 to 2021
Authors: Calimag Aaron Pierre , Veloso Januario Antonio
Published: June 2023

Introduction. Among patients with Acute Myeloid Leukemia (AML), the karyotype at diagnosis is an important prognostic indicator for predicting outcomes. Several studies have been done to identify the most common cytogenetic abnormalities seen in patients in other countries, however, limited studies have been done in our setting.
Objective. The study aims to determine the most common abnormalities present among patients with AML referred for Fluorescence in situ Hybridization (FISH) at the National Kidney and Transplant Institute.
Methodology. The study included 131 adult patients with a mean age of 46. Fluorescence in situ Hybridization was used to identify the following cytogenetic abnormalities: t(8;21), 11q23 (MLL), 16q22 (CBFB-MYH11), t(15;17) (PML/RARA), t(9;22) (BCR/ABL), 7q31 deletion, and Monosomy 7.
Results. FISH was negative in 40% (n=53) of patients. 7q31 deletion is the most frequently identified cytogenetic abnormality among patients with a single abnormality (n=17, 13%) present and is the most frequently identified abnormality among patients with multiple abnormalities (n=26). 7q31 deletion is more frequently observed among patients between the ages 51 to 60 years old and among patients with AML with monocytic differentiation. 22% (n=29) of patients have multiple abnormalities, with the most common abnormalities to occur together are 7q31 deletion and t(8;21) (n=20, 15%). Patients with negative results and patients with multiple cytogenetic abnormalities are commonly seen within the 41 to 50 age group.
Conclusion. The current study provides a single-institution view of the cytogenetic abnormalities among adult Filipino patients with AML using FISH. Further investigation on the clinical history of these patients, with correlation with other methods, as well as epidemiologic studies are needed to better understand the similarities and differences seen from previously reported incidences.
Mountains in Oman Can Store Huge Amounts of CO2 if a Way Can Be Found Into the Tight Rock
Authors: Rassenfoss Stephen
Published: June 2023

Every year, the unusual mix of minerals in the Hajar Mountains near the coast of Oman traps 100,000 tons of carbon in the rock. That estimate is a tiny fraction of the potential of the mountain range and a few others like it in the world.

Based on decades of work by geologists studying this unique formation—known as the Samail Ophiolite—the highly reactive rocks called peridotites can theoretically trap one-half ton of CO2 per ton of that rock in the Hajar Mountains, which extend into the UAE.

A leading voice among the researchers who made the 100,000-ton estimate and developed ideas for removing trillions of tons of carbon from the atmosphere is Peter Kelemen, a professor at Columbia University in New York City.

In a 2019 story in Scientific American, Kelemen said that if it is possible to speed the pace of mineralization “by a factor of a million”—something he thinks is doable with a bit of engineering—“then you end up with a billion tons of CO2 per cubic kilometer of rock per year.”

That potential inspired an Omani entrepreneur, Talal Hasan, to start a company based on the work of Kelemen and colleagues including Juerg Matter, a geochemist now working at the University of Southampton in England. The result was startup 44.01, named for the molecular mass of carbon, where Kelemen serves as an advisor and Matter works part time.

The company’s website describes its plan: “Carbon mineralization in peridotite is happening all the time—we simply speed up the natural process.”

It is a simple-sounding goal. But what it will take to realize the vast potential is anything but simple. In an interview, Kelemen identified why: “The main concern is that the rocks are not very porous.”

Or very permeable, which helps explain why large amounts of highly reactive elements have remained untouched over the 96 million years these reactive minerals have been on land.

The hard rocks in this formation, particularly magnesium-rich olivine, were produced when magma flowed up from the mantle to a mid-ocean ridge where it cooled and the thick layer of rock spread out toward what is now Oman and the UAE, and something extraordinary happened.

When it reached the boundary between two plates, it normally would have returned to the depths of the earth. Instead, it collided with another ophioplite and was ultimately thrust up onto land.

When the theory of plate tectonics transformed geological thinking during the second half of the 20th century, this giant anomaly became a destination for geologists eager to study a rare outcrop of rock from the mantle, including Kelemen.

They also could have gone to New Guinea to look at a similar display of mantle rock or scattered sites in the western US, among other places, where ophiolites are present. But the Hajar Mountains comprise the biggest ophiolite of them all.

Speeding carbon mineralization from geological rates to the pace needed to help stop global warming poses a fundamental problem common to unconventional oil and gas—creating flow paths in tight rock on a limited budget.
Technology Focus: Deepwater Fields (May 2023)
Authors: Alwazzan Amir
Published: June 2023

The dearth of investments, burgeoning stringent environmental regulations, and the looming energy transition all can be serious hindrances to the oil and gas industry. While several questions are being asked about the steadfastness of alternatives to competently meet the growing demand for energy, our industry is mature enough not to trivialize the challenging future. Industry key players and associated organizations have to steer away from flawed assumptions in order to develop realistic, trustworthy, and value-creating strategies to safeguard the business imperative of sustainability.

Considering the admirable perpetual transformation of the deepwater sector and the crucial impetus for change in the industry, the yearning for aggrandized and augmented innovative advancements is needed more now than ever. Such betterments are essential for these developments to increase the success of finding new discoveries, enhance the operability of their fields, and demonstrate the valorization of their assets to sustain their viability. It is a journey that requires cultural organizational transformation rather than attentive changes in order to focus on abating the effects of emerging challenges while emulating the past success of being a hub for adapting sophisticated engineering solutions.

As the deepwater sector embraces the dramatic cutting-edge technical transformation with a digital deluge—artificial intelligence, machine learning, the Internet of things, and automation of operations and remote management—the operators of this sector have an uncontested opportunity to properly adopt and adapt to rigorous operational excellence management systems (OEMS) to balance the investments with the targeted benefits. Effective OEMS can significantly aid identification of bottlenecks; assess the existing processes, procedures, and protocols; and introduce the right solutions for improvement. They are of great benefit for operators to precisely and informatively prioritize in a timely manner areas of pioneering technical advancements needed to assure agility and resilience. It is time for our industry, and the deepwater section in particular, to heed Vince Lombardi’s quote: “Perfection is not attainable, but if we chase perfection, we can catch excellence.”

Recommended additional reading at OnePetro: www.onepetro.org.

SPE 207376 A Novel Approach to First-in-the-World Retrieval of Open-Water Vertical Tree by William Sin Yoong Liew, Petronas, et al.

OTC 31920 Reducing Wellbore Cooling Risks for Operational Success in Highly Depleted Sands in a Deepwater Reservoir by Jessica Avila, Chevron, et al.

OTC 31223 Triple Managed-Pressure-Drilling Technique for Drilling and Intelligent Completion Deployment on an Abandoned Deepwater Well by André Alonso Fernandes, Petrobras, et al.
Impact assessment of Climate change on Subarnarekha watershed of Ranchi stretch in Jharkhand
Authors: Kadave Kiran Prakash , Kumari Neeta
Published: June 2023

The present study deals with the assessment of impact of climate change on water bodies and land use land cover of the study area. Water supply gets adversely affected due to climate change. Subarnarekha River watershed in Ranchi region is selected as the study area. The study area coordinates are 23°27'1.27"N to 23°16'52.39"N and 85°39'51.98"E to 85°18'10.00"E. The selected river stretch is 50.7 kms. The water samples are taken seasonally from twelve sampling points and analysed for basic water quality parameters. The worst affected parameters were salinity and total dissolved solids. Rest of the quality parameters have also shown significant changes with respect to temperature. The climate change induced a change in rainfall and vegetation cover which have eventually caused the change in magnitude and intensity of hydrological variables such as runoff, evapotranspiration and baseflow. The ecosystem in arid and semi-arid areas is degraded as a result of land use/land cover change (LULC) due to climate change. With the use of ArcGIS software, NDWI (Normalized Difference Water Index) maps for pre monsoon and post monsoon is prepared to determine the coverage of water in the Subarnarekha watershed of Ranchi District with respect to time using the satellite data form LANDSAT 8 satellite. The Results of NDWI maps show there was difference in the area of water bodies in the post monsoon season.
Maternal-based paternal DNA methylation remodeling in rice zygote
Authors: Zhou Dao-Xiu , Liu Qian , Ma Xuan , Li Xue , Zhang Xin , Zhou Shao , Zhao Yu
Published: June 2023

Epigenetic reprogramming occurs during reproduction to reset the genome for early development. In flowering plants, mechanistic details of parental methylation remodeling in zygote remain elusive. Analysis of allelic-specific DNA methylation in rice hybrid zygotes and during early embryo development indicates that paternal DNA methylation is predominantly remodeled to match maternal allelic levels upon fertilization, which persists after the first zygotic division. The DMA methylation remodeling pattern supports the predominantly maternal-biased gene expression during zygotic genome activation (ZGA) in rice. However, parental allelic-specific methylations are reestablished at the globular embryo stage and associate with allelic-specific histone modification patterns in hybrids. These results reveal a maternal-controlled paternal DNA methylation remodeling pattern for zygotic genome reprograming and suggest existence of a chromatin memory allowing parental allelic-specific methylation to be maintained in the hybrid.
Development and Validation of a Short-Form Suboptimal Health Status Questionnaire
Authors: Sun Shuyu , Liu Hongzhi , Zheng Guo , Guan Qihua , Wang Yinghao , Wang Jie , Qi Yan , Yan Yuxiang , Wang Youxin , Wen Jun , Hou Haifeng
Published: June 2023

Background
Suboptimal health status (SHS) is a reversible, borderline state between optimal health and disease. Although this condition’s definition is widely understood, related questionnaires must be developed to identify individuals with SHS in various populations relative to predictive, preventive, and personalized medicine (PPPM/3PM). This study presents a short-form suboptimal health status questionnaire (the SHSQ-SF) that appears to possess sufficient reliability and validity to assess SHS in large-scale populations.
Methods
A total of 6,183 participants enrolled from southern China constituted a training set, while 4,113 participants from northern China constituted an external validation set. The SHSQ-SF includes nine key items from the Suboptimal Health Status Questionnaire-25 (SHSQ-25), an instrument that has been applied in Caucasians, Asians, and Africans. Item analysis and reliability and validity tests were carried out to validate the SHSQ-SF. The receiver operating characteristic (ROC) curve was used to identify an optimal cutoff value for SHS diagnosis.
Results
The Cronbach’s α coefficient for the training dataset was 0.902; the split-half reliability was 0.863. The Kaiser–Meyer–Olkin (KMO) value was 0.880, and Bartlett’s test of sphericity was significant (χ2 = 32,929.680, p < 0.05). Both Kaiser’s criteria (eigenvalues > 1) and the scree plot revealed one factor explaining 57.008% of the total variance. Standardized factor loadings for the confirmatory factor analysis (CFA) indices ranged between 0.59 and 0.74, with χ2/dƒ = 4.972, GFI = 0.996, CFI = 0.996, RFI = 0.989, and RMSEA = 0.031. The area under the ROC curve (AUC) was equal to 0.985 (95% CI: 0.983–0.988) for the training dataset. A cutoff value (≥ 11) was then identified for SHS diagnosis. The SHSQ-SF showed good discriminatory power for the external validation dataset (AUC = 0.975, 95% CI: 0.971–0.979) with a sensitivity of 96.2% and a specificity of 87.4%.
Conclusions
We developed a short form of the SHS questionnaire that demonstrated sound reliability and validity when assessing SHS in Chinese residents. From a PPPM/3PM perspective, the SHSQ-SF is recommended for rapid screening of individuals with SHS in large-scale populations.
Effect of salt stress on growth, physio-chemical mechanisms and anatomical alterations of Nigella sativa L
Authors: Akhter Noreen , Ali Ayesha , Aqeel Muhammad , Kausar Abida , Shahnaz Muhammad Muslim , Batool Riffat , Zahra Noreen , Noman Ali , Irshad Muhammad Kashif
Published: June 2023

Nigella sativa L. (Black cumin ) is a medicinal plant, well known for its uses as herbal medicine for the treatment of many health conditions like asthma, inflammation and bronchitis. It is an enriched source of protein, antioxidants and oil contents. In this study, Black cumin was studied for the estimation of osmoprotectants and anatomical features under salt stress. Black cumin seeds were obtained from the Ayub Agricultural Research Institute, Faisalabad (AARI). The experiment was planned in the Botanic Research area of Government College Women University Faisalabad. The experimental design was completely Randomized Design (CRD) with three replicates per treatment. After 4 weeks of germination, plants were treated with different concentrations of salt (control, 50, 100, 150 and 200 mM NaCl). After 6 weeks of salt treatment once in week plants were harvested and analyzed for different morpho-physiological, biochemical and anatomical attributes. Results showed that biomass production (fresh and dry weight) and plant height were decreased in Black cumin under salt stress. However, Plants with high to moderate (50 to 200 mM NaCl) salt concentration reduced root epidermal thickness depicting their adaptation strategy under salt stress. Furthermore, the area of the root and stem cortical cells was reduced in all salt-treated plants which avoid water loss during osmotic stress. Increasing salt stress in the growth-medium of Black cumin showed stunted growth and anatomical modifications.
Association between single nucleotide polymorphisms, TGF-β1 promoter methylation, and polycystic ovary syndrome
Authors: Gao Mengge , Liu Xiaohua , Gu Heng , Xu Hang , Zhong Wenyao , Wei Xiangcai , Zhong Xingming
Published: June 2023

Background
Polycystic ovarian syndrome (PCOS) is a common endocrine and metabolic disease in women. Hyperandrogenaemia (HA) and insulin resistance (IR) are the basic pathophysiological characteristics of PCOS. The aetiology of PCOS has not been fully identified and is generally believed to be related to the combined effects of genetic, metabolic, internal, and external factors. Current studies have not screened for PCOS susceptibility genes in a large population. Here, we aimed to study the effect of TGF-β1 methylation on the clinical PCOS phenotype.
Methods
In this study, three generations of family members with PCOS with IR as the main characteristic were selected as research subjects. Through whole exome sequencing and bioinformatic analysis, TGF-β1 was screened as the PCOS susceptibility gene in this family. The epigenetic DNA methylation level of TGF-β1 in peripheral blood was detected by heavy sulfite sequencing in patients with PCOS clinically characterised by IR, and the correlation between the DNA methylation level of the TGF-β1 gene and IR was analysed. We explored whether the degree of methylation of this gene affects IR and whether it participates in the occurrence and development of PCOS.
Results
The results of this study suggest that the hypomethylation of the CpG4 and CpG7 sites in the TGF-β1 gene promoter may be involved in the pathogenesis of PCOS IR by affecting the expression of the TGF-β1 gene.
Conclusions
This study provides new insights into the aetiology and pathogenesis of PCOS.
2D Quasi-Layered Material with Domino Structure
Authors: Lan Haihui , Wang Luyang , He Runze , Huang Shuyi , Yu Jinqiu , Luo Jingrui , Li Yiling , Lin Jiaxin , Zhang Shunping , Zeng Mengqi , Fu Lei
Published: June 2023

Interlayer coupling strength dichotomizes two-dimensional (2D) materials into layered and non-layered types. Traditionally, they can be regarded as atomic layers intrinsically linked via van der Waals (vdW) forces or covalent bonds, oriented orthogonally to their growth plane. In our work, we report an unprecedented material system that differentiates from layered and non-layered materials, termed quasi-layered domino-structured (QLDS) materials. Considering the skewed structure, the force orthogonal to the 2D QLDS-GaTe growth plane constitutes a synergistic blend of vdW forces and covalent bonds, with neither of them being perpendicular to the 2D growth plane. This feature endows remarkable anisotropy, second harmonic generation enhancement with a staggering susceptibility of 394.3 pm V−1, and outstanding hydrogen evolution reaction catalytic activity with an ultra-low overpotential of 41 mV at 10 mA cm−2 and a modest Tafel slope of 73 mV dec−1. These findings endow further applications arranged in nonlinear optics, sensors, and catalysis.
Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii
Authors: Matsumoto Yasuhiko , Sugiyama Yu , Nagamachi Tae , Yoshikawa Asami , Sugita Takashi
Published: June 2023

Trichosporon asahii is a conditional pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients.Hog1, a mitogen-activated protein kinase, is known to regulate the stress resistance of some pathogenic fungi, but its role in T. asahii has not been investigated. Here, we demonstrated that the hog1 gene-deficient T. asahii mutant is sensitive to high temperature, cell-membrane stress, oxidative stress, and antifungal drugs. The growth of the hog1 gene-deficient T. asahii mutant was delayed at 40˚C. The hog1 gene-deficient T. asahii mutant also exhibited sensitivity to sodium dodecyl sulfate, hydrogen peroxide, menadione, methyl methanesulfonate, UV exposure, and antifungal drugs such as amphotericin B under a glucose-rich condition. Under a glucose-restricted condition, the hog1 gene-deficient mutant exhibited sensitivity to NaCl and KCl. The virulence of the hog1gene-deficient mutant against silkworms was attenuated. Moreover, the cell viability of the hog1 gene-deficient mutant was decreased in the silkworm hemolymph. These phenotypes were restored by re-introducing the hog1 gene into the gene-deficient mutant. Our findings suggest that Hog1 has a critical role in regulating the cellular stress responses of T. asahii.
Absorption, translocation, and accumulation of the fungicide triadimefon in Pak choi (Brassica rapa var chinensis), pepper (Capsicum annuum) and cucumber (Cucumis sativus)
Authors: Song Shanshan , Yu Qing , Yuan Longwei , Li Qin , Hao Qian , Wu Guoli , Li Yu , Lai Yunsong
Published: June 2023

Triadimefon is a typical systemic fungicide, which is widely used in disease management of powdery mildew, rust disease, and southern blight. In this study, we profiled the absorption, translocation, and accumulation in three representative vegetable crops (Pak choi, cucumber, and pepper) by measuring fungicide residue after over-application. The fungicides were applied through entire-plant spraying (EPS), root-irrigation (RI), and middle-leaf-daubing (MLD). The half-life of triadimefon depends on the application method and plant species. The half-life was 5.42 d (Pak choi), 6.86 d (cucumber), and 6.73 d (pepper) in EPS, while it was 4.39 d (Pak choi), 6.30 d (cucumber), and 5.98 d (pepper) in RI. In the treatment of EPS, triadimefon is translocated both upward/outside and downward/inner-side from the daubed leaves in all the three vegetable crops. And the transfer amount in each organ reached a peak at the 2nd day after the fungicide application. In Pak choi, mesophyll deposited more fungicide than petiole; in cucumber and pepper, leaves deposited the biggest amount of triadimefon (about 0.3–0.5 mg·kg− 1), which is followed by stems. Roots and fruits accumulated the least triadimefon. Moreover, triadimefon can impact chlorophyll, root activity, and the activity of superoxide dismutase and catalase in Pak choi, indicating its potential as a plant growth regulator. These studies above provide new information to apply triadimefon safely and efficiently in production of Pak choi, cucumber, and pepper.
Developing Online Communication Training to Request Donation for Vascularized Composite Allotransplantation (VCA): Improving Performance to Match New US Organ Donation Targets
Authors: Siminoff Laura A. , Alolod Gerard P. , McGregor Hayley , Hasz Richard D. , Mulvania Patricia A. , Barker Laura K. , Gardiner Heather M.
Published: June 2023

Background: Approaching families of dying or newly deceased patients to donate organs requires specialized knowledge and a mastery of relational communication. As the transplantation field has progressed, Donation Professionals (DPs) are also leading conversations with family decision makers (FDMs) about the donation of uncommon anatomical gifts, such as face, hands, genitalia, referred to as Vascularized Composite Allotransplants (VCA) without much training or experience. To address the need for training, we adapted and beta tested an evidenced-based communication training program for donation discussions to VCA requests. The overarching goal of Communicating Effectively about Donation for Vascularized Composite Allotransplantation (CEaD-VCA) is to increase the number of VCA transplantations and to improve the socioemotional outcomes of FDMs.
Methods: We developed CEaD-VCA, an online, on-demand training program based on the previously tested, evidenced-based communication skills training program designed to train DPs to have conversations about solid organ donation. The training was modified utilizing data from a national telephone survey with DPs and results of 6 focus groups conducted with members of the general public. The survey and focus groups assessed knowledge, attitudes and barriers to VCA donation. The training was shaped by a partnership with a leading industry partner, the Gift of Life Institute.TM
Results: Using the results as a guide, the existing CEaD training program, consisting of interactive eLearning modules, was adapted to include technical information about VCA, foundational communication skills, and two interactive example VCA donation request scenarios to facilitate active learning. Forty-two DPs from two partner Organ Procurement Organizations (OPOs) participated in the beta test of CEaD-VCA. Pre- and post-test surveys assessed the impact of the training.
Conclusions: The training was scored highly by DPs in effectiveness and ease of use. This project created a standardized, accessible, and comprehensive training for DPs to communicate about VCA donation. CEaD-VCA is an example of how to develop a communication skills training for difficult conversations utilizing input from stakeholders, guided by communication theory. It also demonstrates how gaps in communication skills during medical education can be filled utilizing advanced online Learning Management Systems. The training specifically addresses new CMS rules concerning OPO performance metrics.
Che-1/miR-590-3p/TAZ axis sustains multiple myeloma disease.
Authors: Fanciulli Maurizio , Bruno Tiziana , Catena Valeria , Corleone Giacomo , Cortile Clelia , Cappelletto Maria , bellei barbara , De Nicola Francesca , Gumenyuk Svitlana , Marchesi Francesco , Annibali Ombretta , Blandino Giovanni , Agostino Silvia Di
Published: June 2023

Multiple myeloma (MM) is a blood disease characterized by the malignant accumulation of monoclonal plasma cells in the bone marrow. Among the pathological consequences of MM, defects in osteogenesis characterized by osteolytic lesions, osteopenia, and pathologic fractures are frequently described. Che-1/AATF (Che-1) is a co-transcriptional factor involved in MM transformation and proliferation. Here, we show that Che-1 expression in MM contributes to maintaining low level of WWTR1 (TAZ), a transcriptional coactivator downstream of the Hippo-signaling pathway. We report that the miR-590-3p, deriving from the mRNA splicing of the EIF4H host gene, can target TAZ, contributing to downregulating its expression in MM. Furthermore, we demonstrate by in vivo and in vitro experiments that Che-1 transcriptionally induces EIF4H gene. We provide data to support that miR-590-3p is secreted by MM cells in vitro and in vivo and that it can decrease TAZ levels and the physiological transcriptional expression of osteogenic-related genes, in mesenchymal stem cells committed to osteogenic differentiation. Our findings unveil an unexplored novel Che-1/miR-590-3p/TAZ axis in MM tumorigenesis by providing a rationale to explore the therapeutic potential of metastatic bone lesions.
Analysis of the economics and environmental friendliness of Area-compensated GSHPs in cold regions in China
Authors: wang pengxuan , Wang Yixi , Gao Weijun , Xu Tongyu , Wei Xindong , Shi Chunyan , Qi Zishu , Bai Li
Published: June 2023

Ground Source Heat Pump is widely used in heating and cooling as a clean and efficient energy utilization technology. In cold regions, ground source heat pump systems have significant economic and environmental benefits. In this paper, we analyze the economic and environmental benefits of Area-compensated ground source heat pump systems in cold regions that achieve cooling and heating load balance through area compensation technology to assess their potential for sustainable development and environmental protection. The results show that the Area-compensated ground source heat pump system can operate stably and efficiently over a long period of time, and although the initial investment in the system is high, the initial investment can be recovered in a relatively short period of time by reducing energy consumption and operating costs. Compared with traditional heating and air conditioning systems, ground source heat pump systems can significantly save energy consumption and operating costs. At the same time, with the long-term operation of Area-compensated ground source heat pump systems, carbon dioxide emissions and air pollution can be greatly reduced compared to conventional systems. Area-compensated ground source heat pump systems provide a new way for sustainable development and environmental protection in cold regions by reducing energy consumption and operating costs, greenhouse gas emissions and pollutant release, and support the promotion of ground source heat pump systems in practical applications.
Long-term plasma-activated-water irrigation improves fruit yield in tomato
Authors: Priatama Ryza A. , Beak Hong Kwan , Song Ilchan , Park Soon-ju , Lee Young Koung
Published: June 2023

Plasma agriculture has recently gained popularity owing to its potential applications in precultivation, cultivation, and postharvest processes. Plasma treatments are beneficial seed-priming agents for improving seed germination. The increase plant growth during the seedling stage and enhance fruit storage duration during postharvest treatment. However, little research has been conducted to confirm the long-term effects of plasma-activated water (PAW) treatment on plant cultivation during stages from germination to harvest. Here, we demonstrate the utilization of PAW, generated from a single dielectric barrier discharge (SDBD) device, to understand the functional role of PAW in the cultivation of tomato ‘Micro-Tom’, from germination to harvest. PAW treatment enhanced the growth of young seedlings and increased the number of flowers, plant biomass, and yield compared with the control. Moreover, no adverse effects on the characteristics of fruits produced by PAW-treated plants were observed. These results highlight the potential of PAW as an alternative ecofriendly nutrient source for plant irrigation under nutrient-limiting conditions, during all the stages from seeds to fruits, and offer promising implications for future agricultural practices.
Long-term follow-up after the treatment of obstructive cuspids in the maxilla causing severe root resorption of the lateral incisors: two case reports
Authors: LI Ning , YANG Liu , YANG Qian , WANG Hongning , XU Xiaolin , WANG Tiejun
Published: June 2023

Background
Root resorption of adjacent teeth due to obstructed canines is common, and orthodontic treatment often leads to secondary resorption or even loss of adjacent roots. Clinical reports of long-term stability after treatment are rare.
Case presentation:
This study reports two cases of maxillary cuspid obstruction resulting in severe root resorption of the adjacent lateral incisors. Surgical exposure, orthodontic retraction and alignment of the impacted cuspids successfully retracted the impacted cuspids in both cases, and the resorbed lateral incisors were stable with no significant loosening and normal pulp vitality after treatment and 5 and 10 year follow-up.
Conclusions
Light orthodontic force may be used to move teeth with root resorption of adjacent teeth due to obstructive teeth. The path and direction in which the teeth are moved must be specifically designed so that the adjacent roots are not resorbed and long-term stability can be achieved.
Prevalence and impact of atrial fibrillation in elderly patients with hypertrophic cardiomyopathy
Authors: Song Changpeng , Cui Jingang , Zheng Xinxin , Lu Jie , Guo Xinli , Wang Shuiyun , Huang Xiaohong
Published: June 2023

Background: The study sought to identify the prevalence and the influence of atrial fibrillation (AF) on clinical course and outcomes in elderly patients with hypertrophic cardiomyopathy (HCM).
Methods: From January 2014 to December 2017, 351 consecutive elderly patients diagnosed with HCM in our center were enrolled in this study. Clinical and echocardiographic variables were assessed in association with AF. The effect of AF on all-cause and cardiovascular mortality was evaluated with multivariate Cox proportional hazards models.
Results: Of 351 patients (53% males, mean age 70.6 ± 5.2 years) with HCM, 122 (35%) had AF. AF was related to New York Heart Association (NYHA) class III/IV (OR=2.14, 95%CI: 1.27-3.63, P=0.005), history of stroke (OR=2.39, 95%CI: 1.16-4.91, P=0.018), left atrial dimension (OR=1.13, 95%CI: 1.08-1.19, P<0.001) and pulmonary hypertension (OR=2.91, 95%CI: 1.19-7.10, P=0.019). Over a median of follow-up period of 67 ± 22 months, 72 patients (21%) died. The five-year survival free from all-cause mortality was 83%, 70%, and 89% in the overall HCM population, AF, and non-AF groups, respectively. AF was a significantly independent predictor of all-cause mortality (HR=1.80, 95% CI: 1.02-3.86, P=0.042) and cardiovascular mortality (HR=1.89, 95% CI: 1.08-3.31, P=0.026). In addition, age and NYHA class III/IV were also independent predictors of all-cause and cardiovascular mortality.
Conclusion: AF was common and related to a variety of clinical variables in elderly patients with HCM. As AF was independently associated with poor outcomes, comprehensive managements of AF were important for those elderly patients with HCM and AF.
Comparison of cisplatin-based versus standard preoperative chemotherapy in patients with operable triple-negative breast cancer: Propensity score matching and inverse probability of treatment weighting analysis
Authors: Yamaguchi Ayane , Kawaguchi Kosuke , Kawanishi Kana , Maeshima Yurina , Nakakura Akiyoshi , Kataoka Tatsuki , Takahara Sachiko , Takada Masahiro , Kawashima Masahiro , Sakita-Kawaguchi Nobuko , Kotake Takeshi , Suzuki Eiji , Shimizu Hanako , Torii Masae , Morita Satoshi , ishiguro Hiroshi , Toi Masakazu
Published: June 2023

Purpose:
The efficacy of carboplatin is non-equivalent to that of cisplatin (CDDP) for various tumor types in curative settings. However, the role of CDDP in operable triple-negative breast cancer (TNBC) patients remains unknown. We conducted a multicenter observational study to examine the effects of CDDP added to preoperative chemotherapy in patients with TNBC.
Methods:
This retrospective study consecutively included previously untreated patients with stage I–III TNBC treated with preoperative chemotherapy with or without CDDP. The primary endpoint was distant disease-free survival (DDFS). Propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) were used to minimize confounding biases in comparisons between the two groups.
Results:
A total of 138 patients were enrolled in the study. Of these, 52 were in the CDDP group and 86 in the non-CDDP group. DDFS was significantly better in the CDDP group than in the non-CDDP group (unadjusted hazard ratio (HR) 0.127 and p < 0.001, PSM HR 0.141 and p < 0.003, IPTW HR 0.123 and p = < 0.001). Furthermore, among the patients with residual cancer burden (RCB) class II/III, DDFS was better in the CDDP group than in the non-CDDP group (unadjusted HR 0.192 and p = 0.013, PSM HR 0.237 and p = 0.051, IPTW HR 0.124 and p = 0.059).
Conclusions:
Our study showed that CDDP-containing regimens achieved favorable prognoses in patients with operable TNBC, especially for the RCB class II/III population. Confirmative studies are warranted to elucidate the role of CDDP in TNBC treatment.
Effect of Temperature Humidity Index (THI) on Resting Pattern of Dairy Cows in Different regions of Sri Lanka
Authors: weerasinghe Gayani , Rajapaksha Eranda , Samarakone Thusith
Published: June 2023

The ability to spend more time resting is extremely important for dairy cattle welfare. Through this study, lying behaviour of dairy cows belonged to Up-Country (UP), Mid-Country (MC), Coconut-Triangle (CT) and Western-Province (WP) of Sri Lanka was studied to get an insight into their resting pattern. Resting pattern was assessed in 170 Holstein-Friesian/Jersey crossed, tie-stalled lactating cows (UP = 49, MC = 49, CT = 48 and WP = 24) using electronic data loggers. Proper resting behaviours were attained in UP and MC regions with longer lying time (11.7, 11.3 h/d) and lying bout durations (78.0, 84.9 min) respectively where better THI ranges were observed compared to other regions. WP and CT resulted with lower lying times (9.00, 9.26 h/d) and lying bout durations (48.3, 64.3 min) respectively with higher THI ranges. Cows in all regions prefer to lie on their left side (UP-6.1, MC-6.1, CT-4.4, WP-4.7 h/d) and was significantly differ (P < 0.05) between MC-WP, MC-CT, UP-CT and UP-WP. A negative significant (P < 0.05) correlation was observed between THI and lying behaviour of cows. Unique pattern of diurnal lying down was detected with significant effect (P < 0.05) of time (hour) and Time x Treatment interaction. More lying down was observed in late night and early morning with a peak rest around the hours of 21:00–05:00. Overall, according to the results of the current study, the existing environmental conditions of four regions affected the resting pattern of dairy cows and, identification of possible mechanisms to modify cow’s environment to enhance cow comfort is vital.
Predictive value of combined diagnosis of albumin with alkaline phosphatase or monocyte percentage on the first day of admission to hospital for the risk of death from sepsis
Authors: Zhang Nannan , Tong Wenjing , Gui Yonghui , Yang Peng
Published: June 2023

Objectives: The objective of this study was to evaluate the predictive value of the combined diagnosis of albumin with alkaline phosphatase or monocyte percentage in the risk of death from sepsis.
Methods: A total of 185 patients admitted to the First Affiliated Hospital of Anhui Medical University from February 2022 to January 2023 were selected as the research objects.Laboratory data is selected at admission.To identify potential independent risk factors for sepsis, a multivariate logistic regression analysis was performed.Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive accuracy of ALP, MONO% combined with ALB or the combined diagnosis of all three for mortality outcome in patients with sepsis.
Results: Multiple logistic regression analysis showed that ALB, ALP, and MONO% were independent risk factors for death in patients with sepsis. As shown in Table 3, compared with the diagnosis of ALB alone. ALP or MONO% were diagnosed in combination with ALB or with all three, when all negative predictive values > 96%,the positive predictive value was increased from 36.36% to 42.11%, 46.88% and 47.05%; and the positive likelihood ratio was increased from 4.71 to 6.00 ,7.28 and 7.33 when in the case of negative likelihood ratio < 0.3 for all.
Conclusions: Our study demonstrates that the combined diagnosis of ALP or MONO% with ALB or the combined diagnosis of the three had higher diagnostic value than the single diagnosis of ALB. And the results of this study provide some laboratory basis for the combined laboratory multi-indicator diagnosis of patients with sepsis at risk of death.