bluuebunny/crossref_metadata_2025 huggingface dataset:
Physical Activity in Adolescents Living in Rural and Urban New Caledonia: The Role of Socioenvironmental Factors and the Association With Weight Status
Authors: Wattelez Guillaume , Frayon Stéphane , Caillaud Corinne , Galy Olivier
Published: August 2021

Physical activity (PA) is an important factor for the prevention of overweight and obesity, particularly during adolescence. This study focuses on the understudied adolescent population of New Caledonia with the aim to (1) determine the daily PA levels and estimate the sedentary time through out-of-school sitting time; (2) highlight the influence of sociodemographic and environmental factors, and (3) assess the associations of PA and sitting time with overweight and obesity. A sample of 508 school-going adolescents living in New Caledonia was surveyed about their PA habits using the International Physical Activity Questionnaire–Short Form, as well as about the context in which they usually engage in PA. The influences of the place of living and ethnic community were also investigated. Results indicated that about 66% of the adolescents performed an average of at least 60 min of PA daily. Both Melanesian adolescent boys and girls were more active than Caucasian adolescents but only when they lived in rural areas (females: 115 vs. 93 min/day, p = 0.018; males: 133 vs. 97 min/day, p = 0.018). Indeed, PA was reduced in an urban environment (females: 88 min/day; males: 95 min/day, p = 0.028; rural vs. urban in Melanesian adolescents). Melanesian adolescents also spent less time in out-of-school sitting than Caucasian adolescents independently of where they lived (females: 164 vs. 295 min/day, p < 0.001; males: 167 vs. 239 min/day, p = 0.001). Feeling safe was positively associated with PA levels (females: ORadj = 2.85, p < 0.001; males: ORadj = 4.45, p < 0.001). In the adolescent boys, accessibility to a suitable place was also an important factor (ORadj = 2.94, p = 0.002). Finally, while PA and sitting time were negatively associated with overweight in male adolescents (ORadj = 0.28, p = 0.044 and ORadj = 0.39, p = 0.004), they were not in females. Living in a rural area allowed the Melanesian adolescents to maintain a more active lifestyle with more physical activities and less sitting time. Our results also indicated that safety was an important driver for engagement in PA. The urban environment in New Caledonia appears to be a contributor of a less active lifestyle in adolescents.
Robustness of Bio-Inspired Visual Systems for Collision Prediction in Critical Robot Traffic
Authors: Fu Qinbing , Sun Xuelong , Liu Tian , Hu Cheng , Yue Shigang
Published: August 2021

Collision prevention sets a major research and development obstacle for intelligent robots and vehicles. This paper investigates the robustness of two state-of-the-art neural network models inspired by the locust’s LGMD-1 and LGMD-2 visual pathways as fast and low-energy collision alert systems in critical scenarios. Although both the neural circuits have been studied and modelled intensively, their capability and robustness against real-time critical traffic scenarios where real-physical crashes will happen have never been systematically investigated due to difficulty and high price in replicating risky traffic with many crash occurrences. To close this gap, we apply a recently published robotic platform to test the LGMDs inspired visual systems in physical implementation of critical traffic scenarios at low cost and high flexibility. The proposed visual systems are applied as the only collision sensing modality in each micro-mobile robot to conduct avoidance by abrupt braking. The simulated traffic resembles on-road sections including the intersection and highway scenes wherein the roadmaps are rendered by coloured, artificial pheromones upon a wide LCD screen acting as the ground of an arena. The robots with light sensors at bottom can recognise the lanes and signals, tightly follow paths. The emphasis herein is laid on corroborating the robustness of LGMDs neural systems model in different dynamic robot scenes to timely alert potential crashes. This study well complements previous experimentation on such bio-inspired computations for collision prediction in more critical physical scenarios, and for the first time demonstrates the robustness of LGMDs inspired visual systems in critical traffic towards a reliable collision alert system under constrained computation power. This paper also exhibits a novel, tractable, and affordable robotic approach to evaluate online visual systems in dynamic scenes.
Fast and Slow Adaptations of Interlimb Coordination via Reflex and Learning During Split-Belt Treadmill Walking of a Quadruped Robot
Authors: Aoi Shinya , Amano Takashi , Fujiki Soichiro , Senda Kei , Tsuchiya Kazuo
Published: August 2021

Interlimb coordination plays an important role in adaptive locomotion of humans and animals. This has been investigated using a split-belt treadmill, which imposes different speeds on the two sides of the body. Two types of adaptation have been identified, namely fast and slow adaptations. Fast adaptation induces asymmetric interlimb coordination soon after a change of the treadmill speed condition from same speed for both belts to different speeds. In contrast, slow adaptation slowly reduces the asymmetry after fast adaptation. It has been suggested that these adaptations are primarily achieved by the spinal reflex and cerebellar learning. However, these adaptation mechanisms remain unclear due to the complicated dynamics of locomotion. In our previous work, we developed a locomotion control system for a biped robot based on the spinal reflex and cerebellar learning. We reproduced the fast and slow adaptations observed in humans during split-belt treadmill walking of the biped robot and clarified the adaptation mechanisms from a dynamic viewpoint by focusing on the changes in the relative positions between the center of mass and foot stance induced by reflex and learning. In this study, we modified the control system for application to a quadruped robot. We demonstrate that even though the basic gait pattern of our robot is different from that of general quadrupeds (due to limitations of the robot experiment), fast and slow adaptations that are similar to those of quadrupeds appear during split-belt treadmill walking of the quadruped robot. Furthermore, we clarify these adaptation mechanisms from a dynamic viewpoint, as done in our previous work. These results will increase the understanding of how fast and slow adaptations are generated in quadrupedal locomotion on a split-belt treadmill through body dynamics and sensorimotor integration via the spinal reflex and cerebellar learning and help the development of control strategies for adaptive locomotion of quadruped robots.
Terrain-Based Shadow Correction Method for Assessing Supraglacial Features on the Greenland Ice Sheet
Authors: Leidman Sasha. Z. , Rennermalm Åsa K. , Lathrop Richard G. , Cooper Matthew. G.
Published: August 2021

The presence of shadows in remotely sensed images can reduce the accuracy of land surface classifications. Commonly used methods for removing shadows often use multi-spectral image analysis techniques that perform poorly for dark objects, complex geometric models, or shaded relief methods that do not account for shadows cast on adjacent terrain. Here we present a new method of removing topographic shadows using readily available GIS software. The method corrects for cast shadows, reduces the amount of over-correction, and can be performed on imagery of any spectral resolution. We demonstrate this method using imagery collected with an uncrewed aerial vehicle (UAV) over a supraglacial stream catchment in southwest Greenland. The structure-from-motion digital elevation model showed highly variable topography resulting in substantial shadowing and variable reflectance values for similar surface types. The distribution of bare ice, sediment, and water within the catchment was determined using a supervised classification scheme applied to the corrected and original UAV images. The correction resulted in an insignificant change in overall classification accuracy, however, visual inspection showed that the corrected classification more closely followed the expected distribution of classes indicating that shadow correction can aid in identification of glaciological features hidden within shadowed regions. Shadow correction also caused a substantial decrease in the areal coverage of dark sediment. Sediment cover was highly dependent on the degree of shadow correction (k coefficient), yet, for a correction coefficient optimized to maximize shadow brightness without over-exposing illuminated surfaces, terrain correction resulted in a 49% decrease in the area covered by sediment and a 29% increase in the area covered by water. Shadow correction therefore reduces the overestimation of the dark surface coverage due to shadowing and is a useful tool for investigating supraglacial processes and land cover change over a wide variety of complex terrain.
Progress With Livestock Welfare in Extensive Production Systems: Lessons From Australia
Authors: Windsor Peter Andrew
Published: August 2021

The extensive livestock production industries are vital to the national economy of Australia. Continuing improvements to extensively-raised livestock welfare is desirable, necessary and in some situations mandatory, if the social license for animal sourced food and fiber production is to continue sustainably. However, meeting increasingly high welfare standards is challenging. The changing climate in this millennium, has seen the occurrence of two of the most severe drought periods on record in Australia, resulting in complex welfare issues arising from unforeseen disease, trade and environmental catastrophes. The onset of the first drought coincided with an uncontrolled epidemic of ovine paratuberculosis. It ended just prior to a temporary ban on live export of tropical cattle to Indonesia that induced a major market failure and led to severe morbidity and mortality on some beef properties. The second drought period progressed in severity and culminated in the most extreme bushfires recorded, causing unprecedented levels of mortality, morbidity and suffering in farmed animals and wildlife. Temperature extremes have also caused periodic heat-associated or cold-induced hyopthermia losses, requiring increased vigilance and careful management to reduce both temperature-induced stress during transport and the high ovine peri-parturient losses traditionally observed in extensive sheep farming. Several issues remain controversial, including surgical mulesing of wool sheep to manage flystrike, and the continuing live export trade of sheep and cattle. However, in reviewing the increasingly complex welfare challenges for the extensive livestock population industries that are export trade dependent and remain vulnerable to welfare activism, it appears progress has been made. These include development of prescribed livestock welfare Standards and Guidelines and the introduction of the Exporter Supply Chain Assurance System (ESCAS) to address export concerns. Further, the sheep mulesing crisis led to improved producer welfare attitudes and practices, including pain management during aversive husbandry procedures that is now occurring globally. Finally, innovations in animal welfare surveillance and assessment, are additional encouraging signs that suggest improving change management of extensive farm animal welfare is occurring that provides lessons well-beyond Australian shores.
Leishmaniosis in Rodents Caused by Leishmania infantum: A Review of Studies in the Mediterranean Area
Authors: Alcover M. Magdalena , Riera M. Cristina , Fisa Roser
Published: August 2021

Leishmaniosis infection begins when a phlebotomine sand fly vector inoculates pathogenic protozoan parasites of the genusLeishmaniainto a mammalian host. In the case ofLeishmania infantum, the domestic dog is considered to be the main parasite reservoir, and canine leishmaniosis (CanL) has a high mortality rate in untreated dogs. Hundreds of cases of human leishmaniosis (HL) are reported in the world each year, the incidence in Europe being relatively low. Leishmaniosis control is primarily focused on the dog, combining methods that prevent sand fly bites and boost host resistance to infection. However, these measures are only partially effective and new solutions need to be found. One of the main factors limiting CanL and HL control is the existence of a sylvaticLeishmaniatransmission cycle that interacts with the domestic cycle maintained by dogs. It is suspected that the main reservoir of infection in wildlife are rodents, whose expansion and rapid population growth worldwide is increasing the risk of human and zoonotic pathogen transfer. The aim of this review is therefore to analyze reports in the literature that may shed light on the potential role of rodents in the leishmaniosis transmission cycle in the Mediterranean area. Following the general methodology recommended for reviews, six databases (Google Scholar, Ovid, PubMed, Science Direct, Scopus and Web of Science) were explored for the period January 1995 to December 2020. The results extracted from 39 publications that met the established inclusion criteria were analyzed. It was found that 23 species of rodents have been studied in nine countries of the Mediterranean basin. Of the 3,643 specimens studied, 302 tested positive forL. infantuminfection by serology, microscopy and/or molecular techniques.
Pathological Features in 100 Deceased Patients With COVID-19 in Correlation With Clinical and Laboratory Data
Authors: Mikhaleva L. M. , Cherniaev A. L. , Samsonova M. V. , Zayratyants O. V. , Kakturskiy L. V. , Vasyukova O. A. , Birukov A. E. , Kontorshchikov A. S. , Sorokina A. V. , Sinelnikov M. Y.
Published: August 2021

Background: Autopsies on COVID-19 deceased patients have many limitations due to necessary epidemiologic and preventative measures. The ongoing pandemic has caused a significant strain on healthcare systems and is being extensively studied around the world. Clinical data does not always corelate with post-mortem findings. The goal of our study was to find pathognomonic factors associated with COVID-19 mortality in 100 post-mortem full body autopsies.

Materials and Methods: Following necessary safety protocol, we performed 100 autopsies on patients who were diagnosed with COVID-19 related death. The macroscopic and microscopic pathologies were evaluated along with clinical and laboratory findings.

Results: Extensive coagulopathic changes are seen throughout the bodies of diseased patients. Diffuse alveolar damage is pathognomonic of COVID-19 viral pneumonia, and is the leading cause of lethal outcome in younger patients. Extrapulmonary pathology is predominantly seen in the liver and spleen. Intravascular thrombosis is often widespread and signs of septic shock are often present.

Conclusion: The described pathological manifestations of COVID-19 in deceased patients are an insight into the main mechanisms of SARS-CoV-2 associated lethal outcome. The disease bears no obvious bias in severity, but seems to be more severe in some patients, hinting at genetic or epigenetic factors at play.
The Potential for Sialic Acid and Sialylated Glycoconjugates as Feed Additives to Enhance Pig Health and Production
Authors: Jahan Marefa , Francis Nidhish , Wynn Peter , Wang Bing
Published: August 2021

Swine are one of the most important agricultural species for human food production. Given the significant disease challenges confronting commercial pig farming systems, introduction of a new feed additive that can enhance animal performance by improving growth and immune status represents a major opportunity. One such candidate is sialic acid (Sia), a diverse family of nine-carbon acidic sugar, present in various organs and body fluid, as well as an essential structural and functional constituent of brain ganglioside of humans and animals. Sias are key monosaccharide and biomarker of sialylated milk oligosaccharide (Sia-MOS’s), sialylated glycoproteins and glycolipids in milk and all vertebrate cells. Sias accomplish many critical endogenous functions by virtue of their physiochemical properties and via recognition by intrinsic receptors. Human milk sialylated glycoconjugates (Sia-GC’s) are bioactive compounds known to act as prebiotics that promote gut microbiota development, gut maturation, pathogen resistance, immunomodulation, anti-inflammation and neurodevelopment. However, the importance of Sia in pig health, especially in the growth, development, immunity of developing piglet and in pig production remains unknown. This review aims to critically discuss the current status of knowledge of the biology and nutritional role of Sia and Sia-GC’s on health of both female sow and newborn piglets.
Steroidogenic Enzyme and Steroid Receptor Expression in the Equine Accessory Sex Glands
Authors: Ellerbrock Robyn E. , Podico Giorgia , Scoggin Kirsten E. , Ball Barry A. , Carossino Mariano , Canisso Igor F.
Published: August 2021

The expression pattern and distribution of sex steroid receptors and steroidogenic enzymes during development of the equine accessory sex glands has not previously been described. We hypothesized that equine steroidogenic enzyme and sex steroid receptor expression is dependent on reproductive status. Accessory sex glands were harvested from mature stallions, pre-pubertal colts, geldings, and fetuses. Expression of mRNA for estrogen receptor 1 (ESR1), estrogen receptor 2 (ESR2), androgen receptor (AR), 3β-Hydroxysteroid dehydrogenase/Δ5-4 isomerase (3βHSD), P450,17α hydroxylase, 17–20 lyase (CYP17), and aromatase (CYP19) were quantified by RT-PCR, and protein localization of AR, ER-α, ER-β, and 3βHSD were investigated by immunohistochemistry. Expression of AR, ESR2, CYP17, or CYP19 in the ampulla was not different across reproductive statuses (p > 0.1), while expression of ESR1 was higher in the ampulla of geldings and fetuses than those of stallions or colts (p < 0.05). AR, ESR1 and ESR2 expression were decreased in stallion vesicular glands compared to the fetus or gelding, while AR, ESR1, and CYP17 expression were decreased in the bulbourethral glands compared to other glands. ESR1 expression was increased in the prostate compared to the bulbourethral glands, and no differences were seen with CYP19 or 3β-HSD. In conclusion, sex steroid receptors are expressed in all equine male accessory sex glands in all stages of life, while the steroidogenic enzymes were weakly and variably expressed.
3D Insights into the Effects of Captivity on Wolf Mastication and Their Tooth Marks; Implications in Ecological Studies of Both the Past and Present
Authors: Courtenay Lloyd A. , Herranz-Rodrigo Darío , Yravedra José , Vázquez-Rodríguez José Mª , Huguet Rosa , Barja Isabel , Maté-González Miguel Ángel , Fernández Maximiliano Fernández , Muñoz-Nieto Ángel-Luis , González-Aguilera Diego
Published: August 2021

Human populations have been known to develop complex relationships with large carnivore species throughout time, with evidence of both competition and collaboration to obtain resources throughout the Pleistocene. From this perspective, many archaeological and palaeontological sites present evidence of carnivore modifications to bone. In response to this, specialists in the study of microscopic bone surface modifications have resorted to the use of 3D modeling and data science techniques for the inspection of these elements, reaching novel limits for the discerning of carnivore agencies. The present research analyzes the tooth mark variability produced by multiple Iberian wolf individuals, with the aim of studying how captivity may affect the nature of tooth marks left on bone. In addition to this, four different populations of both wild and captive Iberian wolves are also compared for a more in-depth comparison of intra-species variability. This research statistically shows that large canid tooth pits are the least affected by captivity, while tooth scores appear more superficial when produced by captive wolves. The superficial nature of captive wolf tooth scores is additionally seen to correlate with other metric features, thus influencing overall mark morphologies. In light of this, the present study opens a new dialogue on the reasons behind this, advising caution when using tooth scores for carnivore identification and contemplating how elements such as stress may be affecting the wolves under study.
Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway
Authors: Guo Shichao , Yang Jinyu , Qin Jianpeng , Qazi Izhar Hyder , Pan Bo , Zang Shengqin , Lv Tianyi , Deng Shoulong , Fang Yi , Zhou Guangbin
Published: August 2021

Previously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective was to explore whether melatonin could reduce oxidative stress during in vitro maturation of vitrified-warmed mouse germinal vesicle (GV) oocytes through the Nrf2 signaling pathway or its receptors. During in vitro maturation of vitrified-warmed mouse GV oocytes, there were decreases (p < 0.05) in the development rates of metaphase I (MI) oocytes and metaphase II (MII) and spindle morphology grades; increases (p < 0.05) in the reactive oxygen species (ROS) levels; and decreases (p < 0.05) in expressions of Nrf2 signaling pathway-related genes (Nrf2, SOD1) and proteins (Nrf2, HO-1). However, adding 10−7 mol/L melatonin to both the warming solution and maturation solutions improved (p < 0.05) these indicators. When the Nrf2 protein was specifically inhibited by Brusatol, melatonin did not increase development rates, spindle morphology grades, genes, or protein expressions, nor did it reduce vitrification-induced intracellular oxidative stress in GV oocytes during in vitro maturation. In addition, when melatonin receptors were inhibited by luzindole, the ability of melatonin to scavenge intracellular ROS was decreased, and the expressions of genes (Nrf2, SOD1) and proteins (Nrf2, HO-1) were not restored to control levels. Therefore, we concluded that 10−7 mol/L melatonin acted on the Nrf2 signaling pathway through its receptors to regulate the expression of genes (Nrf2, SOD1) and proteins (Nrf2, HO-1), and mitigate intracellular oxidative stress, thereby enhancing in vitro development of vitrified-warmed mouse GV oocytes.
Advancing Human–Animal Interaction to Counter Social Isolation and Loneliness in the Time of COVID-19: A Model for an Interdisciplinary Public Health Consortium
Authors: Hughes Angela M. , Braun Lindsey , Putnam Alison , Martinez Diana , Fine Aubrey
Published: August 2021

The mental and physical human costs of social isolation and loneliness—and their possible amelioration through human–animal interaction (HAI)—have both received intense attention since the onset of the COVID-19 pandemic, and its lockdowns, quarantines, and related mitigation measures. Concern about society’s “loneliness epidemic”, however, predates the pandemic, as does serious inquiry into HAI as a positive intervention. Recognizing the potential of companion animals to make a difference on an important public health issue, the Consortium on Social Isolation and Companion Animals—a novel partnership of the Human Animal Bond Research Institute (HABRI) and Mars Petcare—launched a joint initiative in 2019 to advance HAI research, address barriers to HAI, and support best practices in bringing together animals and people to ease loneliness. Beginning with a first-ever summit of multidisciplinary thought leaders, this collaboration has already yielded actionable insights and research projects. As a novel partnership initiative in the HAI field, it offers a promising model for future cross-disciplinary forward thinking to elevate HAI for the mutual benefit of companion animals and their welfare, as well as vulnerable human populations.
LSLSD: Fusion Long Short-Level Semantic Dependency of Chinese EMRs for Event Extraction
Authors: Zhai Pengjun , Wang Chen , Fang Yu
Published: August 2021

Most existing medical event extraction methods have primarily adopted a simplex model based on either pattern matching or deep learning, which ignores the distribution characteristics of entities and events in the medical corpus. They have not categorized the granularity of event elements, leading to the poor generalization ability of the model. This paper proposes a diagnosis and treatment event extraction method in the Chinese language, fusing long short-level semantic dependency of the corpus, LSLSD, for solving these problems. LSLSD can effectively capture different levels of semantic information within and between event sentences in the electronic medical record (EMR) corpus. Moreover, the event arguments are divided into short word-level and long sentence-level, with the sequence annotation and pattern matching combined to realize multi-granularity argument recognition, as well as to improve the generalization ability of the model. Finally, this paper constructs a diagnosis and treatment event data set of Chinese EMRs by proposing a semi-automatic corpus labeling method, and an enormous number of experiment results show that LSLSD can improve the F1-value of event extraction task by 7.1% compared with the several strong baselines.
A Millimeter-Wave 4th-Harmonic Schottky Diode Mixer with Integrated Local Oscillator
Authors: Pérez-Escudero José M. , Quemada Carlos , Gonzalo Ramón , Ederra Iñigo
Published: August 2021

In this paper the design and experimental validation of a fourth-harmonic mixer based on Schottky diodes working around 300 GHz is presented. The main novelty of this work consists in the integration of an MMIC-based local oscillator, working around 75 GHz, and a mixer in the same metallic block housing. A prototype has been characterized using the Y-Factor method and yields a best measured conversion loss and an equivalent noise temperature of 14 dB and 9600 K, respectively. This performance is comparable to the state-of-the-art for this type of mixer.
Decoupling Control of Multi-DOF Supporting System of MLDSB
Authors: Zhao Jianhua , Han Fang , Wang Yongqiang , Zhang Xuze , Zhang Guoji , Du Guojun
Published: August 2021

Magnetic liquid double suspension bearing (MLDSB) is composed of an electromagnetic system and hydrostatic system and its bearing capacity and stiffness can be greatly improved. It is very suitable for occasions of medium speed, heavy load, and starting frequently. Due to the gyroscopic effect and interference between the supporting system, the space state of the rotor can be affected and the operation stability of the MLDSB can be reduced greatly. Therefore, the coupling features of the multi-degree of freedom (multi-DOF) system are analyzed and a decoupling controller is designed and verified in the paper. Firstly, the paper introduces the structural characteristics, stress forms, and control regulation mechanism. Then, the mathematical transfer function of the multi-DOF supporting system is established and the coupling principle is revealed. The state feedback decoupling controller is designed by means of a state feedback decoupling, and its decoupling effect is analyzed by the Simulink module. Finally, the single-DOF decoupling system is independently controlled by the use of the root locus method. The coupling characteristics between channel x and channel y are tested experimentally, and the decoupling controller is added and its effect tested. The results show that the state feedback decoupling controller can effectively reduce the coupling degree in the multi-DOF system and convert the multi-DOF coupling problem into a single-DOF independent control problem. The coupling effect of channel x and channel y is reduced by using the decoupling controller, with the subsequent displacement characteristic of the rotor increased, and then the operation stability of the MLDSB is improved greatly. This paper can enrich the support system of the MLDSB and provide a theoretical reference for stability control.
Methodology for the Concept Design of Locally Reinforced Composites
Authors: Berges Julius Moritz , Jacobs Georg , Stein Sebastian , Sprehe Jonathan
Published: August 2021

Locally load-optimized fiber-based composites, the so-called tailored textiles (TT), offer the potential to reduce weight and cost compared to conventional fiber-reinforced plastics (FRP). However, the design of TT has a higher complexity compared to FRP. Current approaches, focusing on solving this complexity for multiple objectives (cost, weight, stiffness), require great effort and calculation time, which makes them unsuitable for serial applications. Therefore, in this paper, an approach for the efficient creation of simplified TT concept designs is presented. By combining simplified models for structural design and cost estimation, the most promising concepts, regarding the cost, weight, and stiffness of TT parts, can be identified. By performing a parameter study, the cost, weight, and stiffness optima of a sample part compared to a conventional FRP component can be determined. The cost and weight were reduced by 30% for the same stiffness. Applying this approach at an early stage of product development reduces the initial complexity of the subsequent detailed engineering design, e.g., by applying methods from the state of the art.
Direct Measurement and Modeling of Intraglottal, Subglottal, and Vocal Fold Collision Pressures during Phonation in an Individual with a Hemilaryngectomy
Authors: Mehta Daryush D. , Kobler James B. , Zeitels Steven M. , Zañartu Matías , Ibarra Emiro J. , Alzamendi Gabriel A. , Manriquez Rodrigo , Erath Byron D. , Peterson Sean D. , Petrillo Robert H. , Hillman Robert E.
Published: August 2021

The purpose of this paper is to report on the first in vivo application of a recently developed transoral, dual-sensor pressure probe that directly measures intraglottal, subglottal, and vocal fold collision pressures during phonation. Synchronous measurement of intraglottal and subglottal pressures was accomplished using two miniature pressure sensors mounted on the end of the probe and inserted transorally in a 78-year-old male who had previously undergone surgical removal of his right vocal fold for treatment of laryngeal cancer. The endoscopist used one hand to position the custom probe against the surgically medialized scar band that replaced the right vocal fold and used the other hand to position a transoral endoscope to record laryngeal high-speed videoendoscopy of the vibrating left vocal fold contacting the pressure probe. Visualization of the larynx during sustained phonation allowed the endoscopist to place the dual-sensor pressure probe such that the proximal sensor was positioned intraglottally and the distal sensor subglottally. The proximal pressure sensor was verified to be in the strike zone of vocal fold collision during phonation when the intraglottal pressure signal exhibited three characteristics: an impulsive peak at the start of the closed phase, a rounded peak during the open phase, and a minimum value around zero immediately preceding the impulsive peak of the subsequent phonatory cycle. Numerical voice production modeling was applied to validate model-based predictions of vocal fold collision pressure using kinematic vocal fold measures. The results successfully demonstrated feasibility of in vivo measurement of vocal fold collision pressure in an individual with a hemilaryngectomy, motivating ongoing data collection that is designed to aid in the development of vocal dose measures that incorporate vocal fold impact collision and stresses.
Redistribution of the Rydberg State Population Induced by Continuous-Spectrum Radiation
Authors: Chervinskaya Anastasia S. , Dorofeev Dmitrii L. , Zon Boris A.
Published: August 2021

We consider the redistribution of the Rydberg state population resulting from multistep cascade transitions induced by radiation with a continuous spectrum. The population distribution is analyzed within the space of quantum numbers n and l. The dynamics of the system are studied using both the numerical solution of kinetic equations and the diffusion approximation based on the Fokker–Planck equation. The main path of the redistribution process is determined.
Deep Minimum and a Vortex for Positronium Formation in Low-Energy Positron-Helium Collisions
Authors: Alrowaily Albandari W. , Ward Sandra J. , Van Reeth Peter
Published: August 2021

We find a zero in the positronium formation scattering amplitude and a deep minimum in the logarithm of the corresponding differential cross section for positron–helium collisions for an energy just above the positronium formation threshold. Corresponding to the zero, there is a vortex in the extended velocity field that is associated with this amplitude when one treats both the magnitude of the momentum of the incident positron and the angle of the scattered positronium as independent variables. Using the complex Kohn variational method, we determine accurately two-channel K-matrices for positron–helium collisions in the Ore gap. We fit these K-matrices using both polynomials and the Watanabe and Greene’s multichannel effective range theory taking into account explicitly the polarization potential in the Ps-He+ channel. Using the fitted K-matrices we determine the extended velocity field and show that it rotates anticlockwise around the zero in the positronium formation scattering amplitude. We find that there is a valley in the logarithm of the positronium formation differential cross section that includes the deep minimum and also a minimum in the forward direction.
Simulation-Based Clarification of Appropriate Factors for Presenting Phosphene in Two Directions Avoiding Electrical Interference
Authors: Kanamaru Manami , Tan Phan Xuan , Kamioka Eiji
Published: August 2021

Walking support systems are essential for blind people. In this study, the presentation of phosphene position is focused on as a method to detect obstacles for blind people. When the phosphene is used in a walking support system, it is necessary to accurately present the phosphene in at least three directions of the visual field. Controlling the presentation of phosphene position has been reported in several previous studies. However, methodologies to present phosphene in multiple directions without any electric interference have not as yet been investigated. In this study, therefore, appropriate stimulation factors are clarified by the simulation of electric field on the eyeball surface which is strongly related to the presentation of phosphene position in the visual field. As a result of the simulation, it was revealed that the distance of each electrode does not give a significant effect to the eyeball surface. However, the phase of alternating current significantly changed the electric field on the eyeball surface. From investigation of the simulation results, it was clarified that the transition of the electric field on the eyeball surface can be controlled using anti-phase stimulation. In addition, the methodology to present the phosphene at least in two directions was verified.
Effect of PACAP on Hypoxia-Induced Angiogenesis and Epithelial–Mesenchymal Transition in Glioblastoma
Authors: Maugeri Grazia , D’Amico Agata Grazia , Saccone Salvatore , Federico Concetta , Rasà Daniela Maria , Caltabiano Rosario , Broggi Giuseppe , Giunta Salvatore , Musumeci Giuseppe , D’Agata Velia
Published: August 2021

Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts different effects in various human cancer. In glioblastoma (GBM), PACAP has been shown to interfere with the hypoxic micro-environment through the modulation of hypoxia-inducible factors via PI3K/AKT and MAPK/ERK pathways inhibition. Considering that hypoxic tumor micro-environment is strictly linked to angiogenesis and Epithelial–Mesenchymal transition (EMT), in the present study, we have investigated the ability of PACAP to regulate these events. Results have demonstrated that PACAP and its related receptor, PAC1R, are expressed in hypoxic area of human GBM colocalizing either in epithelial or mesenchymal cells. By using an in vitro model of GBM cells, we have observed that PACAP interferes with hypoxic/angiogenic pathway by reducing vascular-endothelial growth factor (VEGF) release and inhibiting formation of vessel-like structures in H5V endothelial cells cultured with GBM-conditioned medium. Moreover, PACAP treatment decreased the expression of mesenchymal markers such as vimentin, matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) as well as CD44 in GBM cells by affecting their invasiveness. In conclusion, our study provides new insights regarding the multimodal role of PACAP in GBM malignancy.
DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions
Authors: Žuržul Nataša , Stokke Bjørn Torger
Published: August 2021

In the present paper, we describe a potassium sensor based on DNA-aptamer functionalized hydrogel, that is capable of continuous label-free potassium ion (K+) monitoring with potential for in situ application. A hydrogel attached to the end of an optical fiber is designed with di-oligonucleotides grafted to the polymer network that may serve as network junctions in addition to the covalent crosslinks. Specific affinity toward K+ is based on exploiting a particular aptamer that exhibits conformational transition from single-stranded DNA to G-quadruplex formed by the di-oligonucleotide in the presence of K+. Integration of this aptamer into the hydrogel transforms the K+ specific conformational transition to a K+ concentration dependent deswelling of the hydrogel. High-resolution interferometry monitors changes in extent of swelling at 1 Hz and 2 nm resolution for the hydrogel matrix of 50 µm. The developed hydrogel-based biosensor displayed high selectivity for K+ ions in the concentration range up to 10 mM, in the presence of physiological concentrations of Na+. Additionally, the concentration dependent and selective K+ detection demonstrated in the artificial blood buffer environment, both at room and physiological temperatures, suggests substantial potential for practical applications such as monitoring of potassium ion concentration in blood levels in intensive care medicine.
Shared Genetic Background between Parkinson’s Disease and Schizophrenia: A Two-Sample Mendelian Randomization Study
Authors: Kim Kiwon , Kim Soyeon , Myung Woojae , Shim Injeong , Lee Hyewon , Kim Beomsu , Cho Sung Kweon , Yoon Joohyun , Kim Doh Kwan , Won Hong-Hee
Published: August 2021

Background and objectives: Parkinson’s disease (PD) and schizophrenia often share symptomatology. Psychotic symptoms are prevalent in patients with PD, and similar motor symptoms with extrapyramidal signs are frequently observed in antipsychotic-naïve patients with schizophrenia as well as premorbid families. However, few studies have examined the relationship between PD and schizophrenia. We performed this study to evaluate whether genetic variants which increase PD risk influence the risk of developing schizophrenia, and vice versa. Materials and Methods: Two-sample Mendelian randomization (TSMR) with summary statistics from large-scale genome-wide association studies (GWAS) was applied. Summary statistics were extracted for these instruments from GWAS of PD and schizophrenia; Results: We found an increase in the risk of schizophrenia per one-standard deviation (SD) increase in the genetically-predicted PD risk (inverse-variance weighted method, odds ratio = 1.10; 95% confidence interval, 1.05−1.15; p = 3.49 × 10−5). The association was consistent in sensitivity analyses, including multiple TSMR methods, analysis after removing outlier variants with potential pleiotropic effects, and analysis after applying multiple GWAS subthresholds. No relationships were evident between PD and smoking or other psychiatric disorders, including attention deficit hyperactivity disorder, autism spectrum disorder, bipolar affective disorder, major depressive disorder, Alzheimer’s disease, or alcohol dependence. However, we did not find a reverse relationship; genetic variants increasing schizophrenia risk did not alter the risk of PD; Conclusions: Overall, our findings suggest that increased genetic risk of PD can be associated with increased risk of schizophrenia. This association supports the intrinsic nature of the psychotic symptom in PD rather than medication or environmental effects. Future studies for possible comorbidities and shared genetic structure between the two diseases are warranted.
Preferences of Treatment Strategies among Women with Low-Risk DCIS and Oncologists
Authors: Byng Danalyn , Retèl Valesca , Engelhardt Ellen , Groothuis-Oudshoorn Catharina , van Til Janine , Schmitz Renée , van Duijnhoven Frederieke , Wesseling Jelle , Bleiker Eveline , van Harten Wim , on behalf of the Grand Challenge Precision Consortium
Published: August 2021

As ongoing trials study the safety of an active surveillance strategy for low-risk ductal carcinoma in situ (DCIS), there is a need to explain why particular choices regarding treatment strategies are made by eligible women as well as their oncologists, what factors enter the decision process, and how much each factor affects their choice. To measure preferences for treatment and surveillance strategies, women with newly-diagnosed, primary low-risk DCIS enrolled in the Dutch CONTROL DCIS Registration and LORD trial, and oncologists participating in the Dutch Health Professionals Study were invited to complete a discrete choice experiment (DCE). The relative importance of treatment strategy-related attributes (locoregional intervention, 10-year risk of ipsilateral invasive breast cancer (iIBC), and follow-up interval) were discerned using conditional logit models. A total of n = 172 patients and n = 30 oncologists completed the DCE. Patient respondents had very strong preferences for an active surveillance strategy with no surgery, irrespective of the 10-year risk of iIBC. Extensiveness of the locoregional treatment was consistently shown to be an important factor for patients and oncologists in deciding upon treatment strategies. Risk of iIBC was least important to patients and most important to oncologists. There was a stronger inclination toward a twice-yearly follow-up for both groups compared to annual follow-up.
Different Approaches for Case-Mix Adjustment of Patient-Reported Outcomes to Compare Healthcare Providers—Methodological Results of a Systematic Review
Authors: Sibert Nora Tabea , Pfaff Holger , Breidenbach Clara , Wesselmann Simone , Kowalski Christoph
Published: August 2021

Patient-reported outcomes (PROs) are increasingly being used to compare the quality of outcomes between different healthcare providers (medical practices, hospitals, rehabilitation facilities). However, such comparisons can only be fair if differences in the case-mix between different types of provider are taken into account. This can be achieved with adequate statistical case-mix adjustment (CMA). To date, there is a lack of overview studies on current CMA methods for PROs. The aim of this study was to investigate which approaches are currently used to report and examine PROs for case-mix-adjusted comparison between providers. A systematic MEDLINE literature search was conducted (February 2021). The results were examined by two reviewers. Articles were included if they compared (a) different healthcare providers using (b) case-mix-adjusted (c) patient-reported outcomes (all AND conditions). From 640 hits obtained, 11 articles were included in the analysis. A wide variety of patient characteristics were used as adjustors, and baseline PRO scores and basic sociodemographic and clinical information were included in all models. Overall, the adjustment models used vary considerably. This evaluation is an initial attempt to systematically investigate different CMA approaches for PROs. As a standardized approach has not yet been established, we suggest creating a consensus-based methodological guideline for case-mix adjustment of PROs.
PSMA-Targeting Radiopharmaceuticals for Prostate Cancer Therapy: Recent Developments and Future Perspectives
Authors: El Fakiri Mohamed , Geis Nicolas M. , Ayada Nawal , Eder Matthias , Eder Ann-Christin
Published: August 2021

Prostate cancer (PC) is the second most common cancer among men, with 1.3 million yearly cases worldwide. Among those cancer-afflicted men, 30% will develop metastases and some will progress into metastatic castration-resistant prostate cancer (mCRPC), which is associated with a poor prognosis and median survival time that ranges from nine to 13 months. Nevertheless, the discovery of prostate specific membrane antigen (PSMA), a marker overexpressed in the majority of prostatic cancerous tissue, revolutionised PC care. Ever since, PSMA-targeted radionuclide therapy has gained remarkable international visibility in translational oncology. Furthermore, on first clinical application, it has shown significant influence on therapeutic management and patient care in metastatic and hormone-refractory prostate cancer, a disease that previously had remained immedicable. In this article, we provide a general overview of the main milestones in the development of ligands for PSMA-targeted radionuclide therapy, ranging from the firstly developed monoclonal antibodies to the current state-of-the-art low molecular weight entities conjugated with various radionuclides, as well as potential future efforts related to PSMA-targeted radionuclide therapy.
USP19 and RPL23 as Candidate Prognostic Markers for Advanced-Stage High-Grade Serous Ovarian Carcinoma
Authors: Kang Haeyoun , Choi Min Chul , Kim Sewha , Jeong Ju-Yeon , Kwon Ah-Young , Kim Tae-Hoen , Kim Gwangil , Joo Won Duk , Park Hyun , Lee Chan , Song Seung Hun , Jung Sang Geun , Hwang Sohyun , An Hee Jung
Published: August 2021

Ovarian cancer is one of the leading causes of deaths among patients with gynecological malignancies worldwide. In order to identify prognostic markers for ovarian cancer, we performed RNA-sequencing and analyzed the transcriptome data from 51 patients who received conventional therapies for high-grade serous ovarian carcinoma (HGSC). Patients with early-stage (I or II) HGSC exhibited higher immune gene expression than patients with advanced stage (III or IV) HGSC. In order to predict the prognosis of patients with HGSC, we created machine learning-based models and identified USP19 and RPL23 as candidate prognostic markers. Specifically, patients with lower USP19 mRNA levels and those with higher RPL23 mRNA levels had worse prognoses. This model was then used to analyze the data of patients with HGSC hosted on The Cancer Genome Atlas; this analysis validated the prognostic abilities of these two genes with respect to patient survival. Taken together, the transcriptome profiles of USP19 and RPL23 determined using a machine-learning model could serve as prognostic markers for patients with HGSC receiving conventional therapy.
Microbiota-Gut-Brain Communication in the SARS-CoV-2 Infection
Authors: Manosso Luana M. , Arent Camila O. , Borba Laura A. , Ceretta Luciane B. , Quevedo João , Réus Gislaine Z.
Published: August 2021

The coronavirus disease of 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome 2 (SARS-CoV-2). In addition to pneumonia, individuals affected by the disease have neurological symptoms. Indeed, SARS-CoV-2 has a neuroinvasive capacity. It is known that the infection caused by SARS-CoV-2 leads to a cytokine storm. An exacerbated inflammatory state can lead to the blood–brain barrier (BBB) damage as well as to intestinal dysbiosis. These changes, in turn, are associated with microglial activation and reactivity of astrocytes that can promote the degeneration of neurons and be associated with the development of psychiatric disorders and neurodegenerative diseases. Studies also have been shown that SARS-CoV-2 alters the composition and functional activity of the gut microbiota. The microbiota-gut-brain axis provides a bidirectional homeostatic communication pathway. Thus, this review focuses on studies that show the relationship between inflammation and the gut microbiota–brain axis in SARS-CoV-2 infection.