bluuebunny/crossref_metadata_2025 huggingface dataset:
ESTANDARIZACIÓN DE LOS SUB-PROCESOS DE TRANSPORTE, RECEPCIÓN Y ARCHIVO DEL PROCESO ADMINISTRATIVO EN EMPRESA DE TELECOMUNICACIONES
Authors:   Universidad Libre, Fuentes Rojas Ever Ángel , Bejarano Pinto Juan Camilo , Bejarano Martínez Harold Giovanny
Published: April 2023

La estandarización y/o normalización de procesos es bastante útil para las empresas de hoy en día y más aún si son compañías que operan a nivel nacional como es el caso de una empresa de Telecomunicaciones, para poder saber el verdadero estado de los procedimientos a intervenir en la organización es necesario realizar varias reuniones, entrevistas y algunas encuestas presenciales o virtuales[1]. Se estableció que era necesaria la normalización de tres procesos administrativos como lo son: Recepción, archivo y transporte, se reconoce el trabajo que realiza el talento humano vinculado pero solamente se tienen documentados algunos procedimientos o están desactualizados, el paso a paso a seguir se hace por lógica lo que dificulta la capacitación de los nuevos empleados y la trazabilidad que se requiere ante una no conformidad. Se plantea como objetivo la generación de la estandarización con lineamientos sólidos en cada proceso y sub-proceso, además de ello la creación de nuevos documentos que sirvan como guía y base para la ejecución correcta de cada tarea, bien sea la más compleja e importante hasta la más sencilla. Los resultados de este proyecto permitieron la creación de un 90% de manuales e instructivos, 100% de formatos y la actualización de un 10% de los documentos de la empresa, a su vez se estima la reducción de un 19% de los tiempos en la ejecución de los sub-procesos.
Determinants of Zakat Paying Intentions: Evidences from SMEs’ Workers in Central Java, Indonesia
Authors: Rokhman Wahibur
Published: April 2023

This research aims to discover the factors that influence SMEs workers in zakat paying intention in Central Java, Indonesia. Five factors are studied, including the understanding of zakat, the environment of zakat practices, the credibility of zakat institutions, the function of information, and the roles of religious leaders. This study used 394 SMEs employees who worked in various fields, and the data was processed using multiple regression. The results of this study indicate that the understanding of zakat, the environment of zakat practices, the credibility of zakat institutions, the function of information, and the roles of religious leaders significantly influence zakat paying intention among SMEs workers. The implications of this research need awareness of the managers and stakeholders of zakat institutions need to pay attention to five factors: the understanding of zakat, the environment of zakat practices, the credibility of zakat institutions, the function of information, and the roles of religious leaders because they have a significant impact on the successful development of zakat management ecosystems.
Is Patellar Instability a Contraindication to Semiextended Tibial Nailing?
Authors: Ray Gabrielle S. , Leroy Taryn E. , Ryan Scott P. , Bragg Jack T. , Salzler Matthew J.
Published: April 2023

Case:

We report a 40-year-old woman with undiagnosed patellofemoral instability that worsened 8 months after intramedullary nailing of a distal left tibia fracture in the semiextended position through a partial medial parapatellar approach. Patella stability and asymptomatic knee function were restored after IM nail removal, medial patellofemoral ligament repair, and left tibial tubercle transposition.

Conclusion:

The optimal surgical approach for tibial IM nailing in patients with chronic patellar instability has not been described. Clinicians should be cognizant of the potential for worsening patellofemoral instability in these patients when using the medial parapatellar approach in the semiextended position.
Nonunion of Diaphyseal Humerus Fracture in an Infant
Authors: Beraún-Coronel Luis , Cardenas-Escalante Jhanella , Sinti-Paredes Diego A. , Chamorro-Robles Fredy , Porres-Varona William
Published: April 2023

Case:

A 9-month-old infant girl with Down syndrome presented with an atrophic nonunion of the right humerus diaphysis secondary to birth trauma. Surgical intervention included open reduction and external fixation plus cadaveric cancellous bone allograft and platelet-rich plasma and then was changed to an external fixator in axial compression. At 16 months after surgery, bone healing was achieved.

Conclusion:

Nonunions in infants are rare, and their treatment is a challenge; an adequate vascular supply with good stabilization and reduction are keys to management. We believe that the improvement in reduction and stability under axial compression were the keys to achieve consolidation.
Analysis of Fiqh Textbooks at Madrasah Tsanawiyah Class VII The Tasamuh Approach in the Perspective of Fiqh Wahbah Musthofa Al-Zuhaili
Authors: Ma'arif Syamsul , Hadi Muhammad Fazlurrahman
Published: April 2023

This research is motivated by the increasing fanaticism of the Fiqh school among students, and it's still found that fiqh books are too exclusive to discuss one particular school, without explaining the meaning of other schools. From this background, several questions arise, including what the attitude of tolerance in the Fiqh textbooks for class VII MTs? What is the concept of tolerance in the Tasamuh approach from the perspective of Fiqh Wahbah Musthofa al-Zuhaili? And what is the relevance of the tolerance attitude of Tasamuh's approach in the perspective of Fiqh Wahbah Musthofa al-Zuhaili in the seventh-grade Islamic Jurisprudence textbook? The purpose of this research is to be a reference for the government and other academics on how fiqh textbooks become more inclusive of differences. This type of research uses a literature study with a qualitative descriptive research approach, while the method of analysis is in the form of content analysis. From this research, it can be concluded: First, that not all Wasatiyah Islamic values are explained in Fiqh textbooks. Second, this part of the Fiqh textbook has accommodated different understandings of Islam well. However, it turns out that many parts of the textbook present one interpretation without including a different interpretation. Third, from several research results that researchers have examined that there is a link between intolerance that occurs in Indonesia and the concept of tolerance in textbooks. The researcher also found that most PAI and Tsanawiyah teachers stated that the Fiqh textbooks used in schools did not contain differences and tended to contain only one understanding of Islamic groups.
Technology Innovation of Ginger Spring Machine as a Traditional Jamu for Coronavirus Prevention
Authors: Nely Ana Mufarida  , ABIDIN ASROFUL
Published: April 2023

The COVID-19 disease, including its prevention and treatment, is becoming a lively conversation in the community. There are many ways to protect yourself from the coronavirus. Apart from doing social distancing, consuming traditional drinks can also be a great way to increase endurance. Jamu has been a part of traditional Indonesian medicine for a long time. Herbal medicine is believed to provide a number of health benefits as well as to treat various diseases. Traditional spices are quite effective in maintaining endurance and are one of the solutions against the increasingly aggressive coronavirus (Covid-19) outbreak. Ginger drink is one of the traditional drinks that is easy to make and has the power to increase endurance to avoid the coronavirus (Covid-19). Ginger contains the bioactive compound gingerol which can fight the respiratory syncytial virus that causes respiratory infections. To make the ginger drink, it is necessary to squeeze ginger from the dregs. So far, the processing is done manually by using a grate and squeezing it by hand. People who grate get tired, especially if they have to grate large amounts of ginger. Based on this, appropriate technological innovations need to be applied to increase production yield and quality of squeezed ginger, namely by making the design of "Automatic Ginger Squeezing Machine" which is quite practical and can be used on a household scale with a capacity of 35 kg/hour and a power of 750 Watts. Ginger that is processed in this machine will be immediately separated from the pulp. The production process will be shorter and more efficient because the ginger juice and pulp are separated automatically with a larger capacity. This machine uses stainless steel to ensure the quality of the processed product and maintain the health of the process. This machine works automatically from the beginning to the end of the ginger pressing process.
Comparing methods for estimating water surface elevation between gages in the Lower Mississippi River
Authors: Oliver Amanda , Murphy Catherine , Howe Edmund , Vest John
Published: April 2023

Predicting a water surface elevation (WSElev) at a particular location has a wide range of applications like determining if a levee will overtop or how much a dike notch will increase water flow into a secondary channel. Five existing methods for predicting the water’s surface, (1) daily slope, (2) average slope, (3) River Analysis System (RAS) 1D, (4) RAS 2D, and (5) Adaptive Hydraulics modeling system (AdH), were used to predict the Mississippi River’s daily water surface from 10 October 2014 to 31 May 2016 at Friar’s Point, Greenville, and Natchez gages. The error, calculated as the model-predicted water surface minus the gage-observed water surface, was compared among the methods. The average slope method, using Helena and Fair Landing gages, and the daily slope method, using either Memphis and Helena or Helena and Arkansas City gages, most closely estimated the observed WSElev. The RAS 1D predictions for Friar Point and Greenville produced more accurate estimates than the RAS 2D model and were the only estimates that did not show a pattern of over- or underestimation. When the daily slope method was applied to gages that were farther apart (Memphis and Arkansas City, Arkansas City and Vicksburg, or Vicksburg and Knoxville), the error became greater than most RAS 1D and 2D predictions. The low error and simple calculations of the daily slope and average slope methods using gages <110 river miles apart make these methods useful for calculating current and historic conditions. The lack of over- or underestimation in the RAS 1D predictions (for locations away from the edges of the model area) make this method a better choice for predicting average WSElevs and a good choice for forecasting future WSElevs.
Visual Data Mining: Digital Visual Activities In Network Society
Authors: Kristiyono Jokhanan , Islami Maulia Jayantina , Sari Ratna Puspita , Suprihatin Suprihatin
Published: April 2023

This article describes scientific discoveries that drive the phenomenon of visual cultural communication media in networked societies. Many types of research on visual culture have focused more on visual arts and mass media. However, few have yet addressed the problem of people's digital activities in searching for visual information on the Internet ( Visual search engine as a resource for visual data mining The amount of digital information, including visual information, is increasing exponentially.This research uses a literature study method with a qualitative approach. The primary data used in this study are studies on visual culture and visual search engines published in international peer-reviewed journals. Analysis of digital behavior and visual culture in a networked society is described as a research topic in previous research results. The conclusions drawn indicate that there is still a relatively deep and broad analysis of the study of visual culture when using digital communication media. There are few research studies on visual culture and digital activities in connected communities, especially people's visual information search behavior.
Association of Acute Myocardial Infarction with Ossification of the Posterior Longitudinal Ligament in Korea: A Nationwide Longitudinal Cohort Study
Authors: Kim Jaehwan , Kim Chai Yoon , Kim Jeong Gyun , Kim Hakyung , Sheen Seung Hun , Han Inbo , Sohn Seil
Published: April 2023

Objective: This nationally matched longitudinal study aimed to investigate the relationship between acute myocardial infarction (AMI) and ossification of the posterior longitudinal ligament (OPLL) in Korea.Methods: We collected patient data from January 1, 2004 to December 31, 2015 from the National Health Insurance Service Health Screening Cohort. Patients with OPLL were defined as patients with the International Classification of Diseases, Tenth Revision code M48.8 (other specified spondylopathies) and were newly diagnosed through computed tomography imaging. The OPLL group had a total of 1,289 patients. The control group included 6,445 people. Utilizing the Kaplan-Meier technique, The incidence of AMI in both groups was estimated. A Cox proportional-hazards regression analysis was used to compute the AMI hazard ratio.Results: After controlling for age and sex, the hazard ratio of AMI in the OPLL group was 2.065 (95% confidence interval [CI], 1.228-3.474). The adjusted hazard ratio in the OPLL group was 2.209 after restricting the sample for demographics and concomitant medical conditions (95% CI, 1.311-3.721). In a subgroup analysis, the incidence of AMI was substantially greater in the OPLL group, which included women younger than 65 years and without hypertension, diabetes, or dyslipidemia.Conclusion: This nationwide longitudinal study found that patients with OPLL were at higher risk of AMI.
Organic Geochemistry of Carbonate Rocks, Silicites and Black Shales of the Vendian (Ediacaran) Khatyspyt Formation, Northeastern Siberia
Authors: Melnik D.S. , Parfenova T.M. , Grazhdankin D.V. , Rogov V.I.
Published: April 2023

—The paper presents the results of the study of organic geochemistry of carbonate rocks, silicites, and calcareous mudstones of the Vendian (Ediacaran) Khatyspyt Formation in the northeast of the Siberian Platform. The distribution of dispersed organic matter (OM) is shown to be uneven. The formation comprises carbonate-dominated intervals depleted in OM, with total organic carbon content (TOC) < 0.1%, or weakly enriched in OM (TOC up to 0.4%), interstratified with carbonate-shale, carbonate-shale-siliceous, carbonate-siliceous and siliceous intervals enriched in OM (TOC up to 1–4%). There is also a thin-layered calcareous mudstones anomalously enriched in OM (black shales with TOC at 10%) cropping out in the Khorbusuonka River valley (unit thickness 10 cm). The redox conditions in the water column varied during deposition of the Khatyspyt Formation. Certain intervals characterize a stratified water column and euxinic bottom water conditions. During the study, the assessment was conducted of the OM catagenesis and the generative potential of the Khatyspyt Formation. Based on the results of geochemical study and the synthesis of information on the geological structure of sedimentary succession in the northeastern Siberian Platform, the Khatyspyt Formation has been evaluated as one of the hydrocarbon sources for the territory.
A study on digital twin technology of Human-machine integration cross-belt Sorting System
Authors: Qu Yanbo , Zhao Ning , Zhang Haojue
Published: April 2023

So far nearly 110 billion pieces of express items have been handled by Chinese express delivery industry, with an average annual growth of 200%. Cross-belt sorting system serves as the most important express item sorting system and most of the sorting work has been automated fulfilled. However, operators are still needed and working pressure arisen with the growth of express items. Under the context of the industry 5.0, new technologies should be utilized to enhance both welfare of the workers and working efficiency of the cross-belt system. However, it’s hard to strike a good balance between express item handling efficiency and the happiness of operators. Digital twin technology bring new solution to this problem. Based on the ascertained human-machine features of the distribution center, the human-machine integration digital twin frame, consisting of physical space, virtual space, twin data center and supporting & application services, were proposed. The closed-loop interactions among biological human body, virtual human body, virtual equipment and physical equipment were achieved. The express item distribution center operation system was established based on the digital twin technologies. Besides, the physical-level data collection and storage, the human body fatigue efficiency conversion process on services levels and the virtual-level predication & decision-making were elaborated. Finally, methods to establish the cross-belt sorting system for the express item distribution center based on digital twin technologies were proposed. Feasibility of the aforesaid methods was verified by a simulation case study.
Chlorpyrifos in Fish and Seals from the Canadian Arctic
Authors: Muir Derek , Evans Marlene , Gamberg Mary , Houde Magali , Kirk Jane , Stern Gary , Ross Tyler , Thiemann Gregory , Morris Adam
Published: April 2023

Chlorpyrifos (CPY), a widely used organothiophosphate insecticide, has been proposed for listing on Annex A of the Stockholm Convention on Persistent Organic Pollutants. While CPY has been widely measured in Arctic air and seawater, information on bioaccumulation of CPY in aquatic food webs in the Arctic is very limited. This study presents results of additional monitoring data on CPY in fish and marine mammal samples from the Canadian Arctic/sub-Arctic based on ongoing studies under Canada’s Northern Contaminants Program. The majority of the CPY data was from samples collected from 2011 to 2021 and analysed using USEPA Method 1699, involving quantification by high resolution mass spectrometry. The available dataset showed low detection frequencies of CPY ranging from zero in arctic grayling (Thymallus arcticus) to 52% in arctic cod (Boreogadus saida). To enable statistical analysis the non-detects were substituted with ½ the detection limit (½ DL) based on the assumption that the non-detects were not zero and previous studies showing CPY was consistently detected in water and air in the Canadian Arctic. Largest geometric mean CPY concentrations were found in lake whitefish (Coregonus clupeaformis) muscle (0.56 ng/g wet wt) while burbot (Lota lota) liver had the highest maximum concentration (8.2 ng/g). Log10 CPY concentrations (with ½ DL substitution) were significantly correlated with % lipid and length in lake trout (Salvelinus namaycush) muscle and with % lipid in burbot liver. Geomean concentrations in ringed seal (Pusa hispida) blubber from 8 communities ranged from 0.005 to 0.605 ng/g and showed no geo-spatial trends. Overall, the results indicate widespread but very low level contamination of Arctic fish and seals by CPY.
Therapeutic effects of the stromal vascular fraction and adipose-derived stem cells on osteoarthritis in an experimental rabbit model
Authors: Anjiki Kensuke , Matsumoto Tomoyuki , Kuroda Yuichi , Fujita Masahiro , Hayashi Shinya , Nakano Naoki , Maeda Toshihisa , Takashima Yoshinori , Kikuchi Kenichi , Ikuta Kenmei , Onoi Yuma , Tachibana Shotaro , Suda Yoshihito , Wada Kensuke , Matsushita Takehiko , Kuroda Ryosuke
Published: April 2023

Background The stromal-vascular fraction (SVF), comprising heterogeneous cell populations and adipose-derived stem cells (ADSCs), has therapeutic potential against osteoarthritis (OA); however, the underlying mechanism remains elusive. This study investigated the therapeutic effects of the heterogeneous cells in rabbit SVF on rabbit chondrocytes and compared them with those of two different ADSCs adjusted for their stem cell content.Methods Animals were divided into control, low-dose ADSCs (lADSC; 1×104 ADSCs), high-dose ADSCs (hADSC; 1×105 ADSCs), and SVF (1×105 SVF) groups. SVF and ADSCs isolated from rabbits were autografted into the knees after OA onset, and the animals were sacrificed 8 and 12 weeks after OA onset. OA progression and synovitis was analyzed by macroscopic and histological analyses, and the expression of collagen type II, SOX9, MMP-13, CD86 (M1) and CD163 (M2) was assessed using immunohistochemical and real-time polymerase chain reaction (RT-PCR). In vitro, chondrocytes were either co-cultured with SVF or lADSCs or alone (control), and the pellet size was evaluated. TGF-β and IL-10 concentrations in the medium were evaluated using an enzyme-linked immunosorbent assay. Additionally, immune-fluorescent double staining was performed for SVF and lADSCs to stain macrophages with F4/80, CD86, and CD163.Results OA progression was significantly suppressed, and synovitis was reduced in the SVF and hADSC groups. Compared to those in the control and lADSC groups, immunohistochemistry and RT-PCR revealed higher expression of collagen type Ⅱ and SOX9, lower expression of MMP-13 in cartilage, lower macrophage M1/M2 ratio, and higher expression of TGF-β, IL-10 in the synovium in the SVF and the hADSC groups. In the SVF group, the pellet size and TGF-β and IL-10 concentrations were significantly higher than those in the control and lADSC groups. Immune-fluorescent double staining revealed a significantly higher number of M2 than M1 macrophages in SVF.Conclusions The therapeutic effects of SVF and hADSCs superior on chondrocytes was superior with enhanced anabolic and inhibited catabolic factors than those of controls and lADSCs. Heterogeneous cells, mainly M2 macrophages in SVF, contributed to the therapeutic effect on chondrocytes and knee joint homeostasis by enhancing the growth factors and chondrocyte protective cytokines secretion.
MCDA-CRISPR-Cas12-based diagnostic platform for detection of Mycoplasma Pneumoniae Targeting the CARDS gene
Authors: Yang Tingyu , Xiao Jing , Li Feina , Wang Yonghong , Ma Qi , Chen Yuying , Wang Yi , Li Jieqiong
Published: April 2023

Mycoplasma pneumoniae (MP) is a leading cause of pediatric community-acquired pneumonia (CAP) and advanced techniques for Mycoplasma pneumoniae pneumonia (MPP) diagnosis are urgently needed. Here, a novel diagnostic test combined multiple cross displacement amplification (MCDA) with CRISPR-Cas12a system targeting the CARDS gene of MP, termed MP-MCC, were developed for MPP detection. The MCDA assay was employed for nucleic acid amplification, and the CRISPR-Cas12a/CrRNA complex was used to decode the amplification products. Then, the detection result was observed via real-time fluorescence. The optimal conditions for our assay include a MCDA reaction at 61°C for 40 min and a CRISPR detection at 37°C for ~ 5 min. The results showed that reaction products were detectable from as little as 10fg of pure MP templates and from approximately 10 copies of plasmids containing CARDS genes. The specificity in detecting MP strains was 100%, and there was no cross-reaction to non-MP strains. Furthermore, our assay was further validated using clinical samples, which offered high sensitivity and specificity for MPP diagnostic. Taken together, the detection technique developed here has advantages on rapidity, sensitivity and specificity for MP detection, which could be used as a potential tool for clinical MPP diagnosis.
The nurses' perceptions of the obligatory continuing professional development program in a large tertiary hospital
Authors: Zaitoun Rasha Abu , Battat Maher , sakleh Israa , Zabin Loai , ihudiebube-splendor Chikaodili
Published: April 2023

Background
A crucial strategy for preserving skills and job motivation is continuing professional development. This research aims to evaluate how nurses at NNUH perceived the hospital's mandatory continuing professional development (CPD) program.
Method
The study was carried out in a tertiary hospital in Nablus, Palestine. A cross-sectional quantitative descriptive study was conducted from July to September 202 for 202 full-time and in-duty nurses at NNUH using the Chikaodili Ihudiebube-splendor questionnaire.
Results
The Web survey of 202 nurses shows that the nurse’s perception of mandatory continuing education for professional development is statistically significant with age, sex and marital status. The highest mean score was 3.29 for "Continuing education should be made mandatory for nurses," and 2.39 for that mandatory CPD programs are useful for nurses. However, the overall perceived relevance of these items was not positive (2.39 ± 0.77). The mean score for all items related to the reason for participation was greater than 2.5 and the participants had a significant positive score (3.19 ± 0.49). The remaining perceived obstacles to requiring ongoing education for professional growth were greater than 2.5, and the total perceived obstacle was positive (2.80 ± 0.66). Only one item, "Lack of employer's cooperation," fell below the cut-off point of 2.25.
Conclusions
Nurses mostly perceive that continuing education should be made mandatory for nurses, and the things I learn in mandatory continuing education programs will be useful to me. There are many reasons for participating in required education programs, and finally, nurses face many barriers to required continuing education for professional development at NNUH.
Implications
Special attention and targeted support should be provided to promote the continuing professional development of nursing staff. Nurses maintained a positive attitude towards continued professional development, but faced challenges.
Assessment of Multi-Perspective and Multi-Sensor Data for Urban Strata Mapping
Authors: Ramlal Bheshem , Archibald Daniel , Al-Tahir Raid , Sutherland Michael , Davis Dexter
Published: April 2023

Many countries have seen a significant increase in the number of high-rise and multi-story buildings with strata units with the shrinking availability of horizontal land space in urban areas. Most existing cadastres record tenure and associated information in a two-dimensional format, and therefore will need to be upgraded to 3D cadastres to facilitate the recording of title information for these strata units. The geospatial aspects of implementing a 3D cadastre require cost-effective, rapid, accessible, non-labor intensive, and accurate means by which complete data may be collected to model strata units. Existing data sources such as Building Information Models (BIMs) have been used to delineate these units. However, BIMs are not easily accessible in developing countries. There is no single spatial data source that can easily fulfill all the criteria of a 3D data source for mapping urban strata boundaries. However, leveraging multi-perspective and multi-sensor data can theoretically serve this purpose. In this paper, we report on research conducted to demonstrate the feasibility of using low cost and accessible cell phone data, integrated with higher accuracy terrestrial laser scan data, and drone aerial imagery to produce a multi-perspective 3D data source. It was found that the integration of these data sets as a 3D data source, resulted in absolute accuracies within centimetres and decimetres in the horizontal plane and within millimetres in the vertical plane. Overall, the integration of these three sources of data may be appropriate in meeting the needs for a low-cost source of 3D data.
Health-related internet use among outpatient oncology patients and the impact of the Covid-19 pandemic
Authors: Seynstahl Sarah , Erickson Nicole , Jost Nicole , Wuerstlein Rachel , Fey Theres , Nasseh Daniel , Casuscelli Jozefina , Theurich Sebastian , Heinemann Volker
Published: April 2023

Objective The aim of this study was to assess eHealth usage among oncology patients undergoing infusion therapy. Methods We used an extended version of the validated 21-item questionnaire “Use of information and communication technologies questionnaire (ICT)” developed by Seifert et al., in order to assess patient-reported information related to general internet abilities including health-related applications.
Results 303 patients participated. 299/303 participants reported regular internet usage. 72.6% (217/299) use the internet to search for health-related information and 79.1% (235/297) expressed readiness to communicate digitally with health care providers. Decreasing age and higher internet literacy correlated with a more frequent use of eHealth applications (p<0.001). Users of IOS tended to use therapy apps more frequently compared to users of other operating systems (p=0.034). 25% (68/275) reported increased internet usage during the pandemic.
Conclusion The majority of patients were regular internet users and expressed an openness to eHealth. Factors such as internet literacy and average age are important to consider when implementing new eHealth applications. Despite the positive influence of the pandemic on internet usage, there remains a gap between self-reported readiness and real use of eHealth applications.
Practice Implications eHealth applications should be introduced slowly into clinical settings with provided help.
eHealth applications should be introduced slowly into clinical settings regular support is provided
It is important to carefully consider the population’s characteristics to ensure successful implementation of eHealth applications
The pandemic is a driver for the use of the Internet including eHealth
Research on Carbon Allowance Allocation for Public Buildings in China Based on the Target of Carbon Emission Intensity Reduction in 2030
Authors: Zhang Luqi , Liu Yisheng , Du Zhuoqun
Published: April 2023

Due to the increasing energy crisis and climate warming, carbon emission reduction has attracted the attention of all countries. Carbon trading is an effective way to reduce carbon emissions. As one of the three major carbon emitters, the construction sector urgently needs to establish and improve the construction carbon trading market. Since the allocation of carbon emission rights is the first step to carbon trading, it is necessary to reasonably allocate carbon emissions to each province. This paper establishes a set of carbon emission allocation methods based on the principles of equity, efficiency, historical responsibility, emission reduction capacity and environmental level according to the relative emission reduction action target of 2030 proposed by the Chinese government. Correspondingly, urban population, carbon emission efficiency calculated by DEA model, historical cumulative CO2 emissions, ratio of R&D expenditure to GDP and forest coverage are selected as indicators and the entropy weight method is applied to allocate the amount of CO2. Taking the achievement of China's carbon peak target as an example, carbon emission quotas for public buildings in 2030 were calculated for each province. After verification of Gini coefficient and contribution coefficient, the allocation scheme is derived to be compatible with the economy, population and environment. The results show that the CO2 allocation shows a trend of gradual decrease from southeast to northwest, with Guangdong and Jiangsu having the highest carbon quotas and Qinghai and Xinjiang having the lowest. 33.3% of the provinces are under pressure to reduce emissions, with Xinjiang having the highest pressure to reduce emissions, and economically developed regions like Beijing, Zhejiang and Guangdong still face some pressure to reduce emissions. Finally, the allocation method proposed in this paper can be used as a reference for the building sector, and the allocation scheme can help policy makers in each province to formulate an effective and differentiated emission reduction strategy that ensure the successful achievement of emission reduction targets.
Surfactant deficiency increases SARS-CoV-2 canonical and non-canonical viral entry to lung epithelial cells and exacerbates intrapulmonary inflammatory responses
Authors: Leibel Sandra , McVicar Rachael , Kwong Elizabeth , Clark Alex , Alvarado Asuka , Grimmig Bethany , Nuryyev Ruslan , Young Randee , Lee Jamie , Peng Weiqi , Zhu Yanfang , Griffis Eric , Nowell Cameron , James Brian , Alarcon Suzie , Malhotra Atul , Gearing Linden , Hertzog Paul , Galapate Cheska , Galenkamp Koen , Commisso Cosimo , Smith Davey , Sun Xin , Carlin Aaron , Croker Ben , Snyder Evan
Published: April 2023

The prevalence of “Long COVID”, including among vaccinated patients, is just one of the conundrums that indicate how much remains unknown about the lung’s response to viral infection, particularly to SARS-CoV-2 for which the lung is the point of entry. Therefore, we used an in vitro human lung system to enable a prospective, unbiased, sequential single cell level analysis of pulmonary cell responses following infection by multiple strains of SARS-CoV-2. By starting with human induced pluripotent stem cells (hiPSCs) and emulating lung organogenesis, three-dimensional lung organoids were generated and infected in which several unexpected but pertinent insights emerged. First, SARS-CoV-2 tropism is much broader than previously believed: most lung cell types can be infected, if not through a canonical receptor-mediated route (e.g., via ACE2) then via a non-canonical “backdoor” endocytosis/micropinocytosis route. Such entry can be abrogated by FDA-approved endocytosis blockers, suggesting novel adjunctive therapies. Regardless of route-of-entry, the virus triggers a heretofore unrecognized lung epithelial cell-intrinsic autonomous innate immune response involving interferons and cytokine/chemokine production in the absence of hematopoietic cells or their derivatives. The virus can spread rapidly throughout human lung organoid cell cultures resulting in mitochondrial apoptosis mediated by the pro-survival protein Bcl-xL. This host cytopathic response to the virus may help explain persistent inflammatory signatures in a dysfunctional pulmonary environment of long COVID. The host response to the virus is, in part, dependent on the presence of pulmonary Surfactant Protein-B (SP-B), which plays an unanticipated role in signal transduction, viral resistance, dampens systemic inflammatory cytokine production, and minimizes the induction of apoptosis.
Extreme hepatectomy with modified ALPPS in a rat model: Gradual portal vein restriction associated with hepatic artery restriction
Authors: He Xiaoqin , Zhang Yuefeng , Ma Peng , Mou Zuo , Wang Wei , Yu Kaihuan , Wang Weixing
Published: April 2023

Background & aim: Associating liver partition and portal vein ligation (PVL) for staged hepatectomy (ALPPS) is a creative strategy for enlarging the future liver remnant (FLR) and increasing the tumor resectability rate. However, the indications for ALPPS must have a certain limit when the FLR is too small. We aimed to establish a modified ALPPS model with more widen applicability in rats.
Methods: An extreme ALPPS model was established in rodents with only a 6.5% FLR. The portal vein (PV) was subjected to restriction to different degrees, then the portal vein pressure (PVP) was measured. Then, different modifications of ALPPS, including hepatic artery restriction (HAR), gradual portal vein restriction (GPVR), and GPVR-associated HAR (HAR+GPVR), were applied in the extreme ALPPS models.
Results: PVL or PVR provoked an immediate increase in the PVP. The PVP in the PVR -1.28 mm, PVR -0.81 mm, PVR -0.63 mm, and PVL groups was 11.05±1.57 cmH2O, 16.18±1.92 cmH2O, 20.66±1.99 cmH2O, and 24.10±3.33 cmH2O, respectively, and the corresponding 3-day survival rate was 100%, 90.09%, 36.33% and 0, respectively. Then, in the extreme ALPPS model, the growth ratio of the FLR in the control, HAR, GPVR, and HAR+GPVR groups was 0.43±0.21, 0.50±0.16, 4.80±0.86, and 7.40±2.56, and as a consequence, the corresponding 30-day survival rate was 9.09%, 15.38%, 84.61% and 92.90%, respectively.
Conclusion: ALPPS itself has a limit, and high PVP after PVL contributes to postoperative death in the extreme ALPPS model. Furthermore, a modified method for extreme ALPPS is proposed, i.e., GPVR+HAR in place of PVL, which significantly improves the survival rate of extreme hepatectomy in rat models.
Thymoquinone-Loaded Self Nano-Emulsifying Drug Delivery System against Ischemia/Reperfusion Injury
Authors: BAHLOUL Badr , Chaabani Roua , Zahra Yosri , Kalboussi Nesrine , Kraiem Jamil , Sfar Souad , Mignet Nathalie , Abdennebi Hassen Ben
Published: April 2023

In the present study, a self nano-emulsifying drug delivery system (SNEDDS) was developed to evaluate the efficiency of TQ in hepatic ischemia/reperfusion. SNEDDS was pharmaceutically characterized to evaluate droplet size, morphology, zeta potential, thermodynamic stability, and dissolution/diffusion capacity. Animals were orally pre-treated during 10 days with TQ loaded SNEDDS. Biochemical analyses, haematoxylin-eosin staining, indirect immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR) were carried out to assess cell injury, oxidative stress, inflammation, and apoptosis. The TQ formulation showed good in vitro characteristics, including stable nanoparticle structure and size with high drug release rate. In vivo determinations revealed that TQ loaded SNEDDS pre-treatment of rats maintained cellular integrity by decreasing transaminase (ALT and AST) release and preserving the histological characteristics of their liver. The antioxidant ability of the formulation was proven by increased SOD activity, reduced MDA concentration and iNOS protein expression. In addition, this formulation exerted an anti-inflammatory effect evidenced by reduced plasma CRP concentration, MPO activity, gene expressions of TLR-4, TNF-α, NF-κB, and IL-6. Finally, the TQ loaded SNEDDS formulation promoted cell survival by enhancing the Bcl-2/Bax ratio. In conclusion, our results indicate that TQ encapsulated in SNEDDS significantly protects rat liver from I/R injury.
Green synthesis of TiO2 nanoparticles for furfural production by photohydrolysis of unexplored residual biomass: the nejayote
Authors: López-Mercado Janneth , González-Domínguez Martha-Isabel , Reynoso-Marin Francisco-Javier , Acosta Brenda , Smolentseva Elena , Nambo Apolo
Published: April 2023

Green synthesis is characterized by using plants’ secondary metabolites (S-Met) to reduce metal ions into metallic atoms, which are subsequently nucleated and agglomerated, forming the nanoparticles (NPs). Thereby, the significant diminishing in producing toxic waste during the green synthesis approach may be considered an environmentally friendly alternative. In the present work, titania (TiO2) NPs were greenly synthesized using Ricinus Communis (RC), Moringa Oleifera (MO) or Bougainvillea Spectabilis (BS) plant extracts. Obtained nanoparticles were characterized using XRD, SEM, EDS, BET, XPS and UV-vis techniques. The physicochemical and electronic properties of synthesized nanoparticles were improved concerning the reference material. The surface area increased up to 17 times, accompanied by a decrease in crystal size (∼50%) and gap energy value. Furthermore, the photocatalytic performance of the obtained samples was evaluated in the furfural production from nejayote, an unexplored industrial residual biomass. The furfural yield was twice higher using the sample obtained from the BS metabolites than those obtained with the other S-Met, attributed to the preferable formation of xylose over other pentoses. This work proved the viability of nanomaterial synthesis using common plants abundant in Latin-American applied for the waste transformation coming from an overall process such as nixtamalization, the nejayote.
pH-dependent crystal structures of a humanised therapeutic antibody Fab fragment: impact on molecular dynamics simulations and comparison to solution structures
Authors: Tang Jiazhi , Zhang Cheng , Castillo Nuria Codina , Lalaurie Christophe , Gao Xin , Dalby Paul , Kozielski Frank
Published: April 2023

Antibody-fragment (Fab) therapy development has the potential to be accelerated by computational modelling and simulations that predict their target binding, stability, formulation, manufacturability, and the impact of further protein engineering. Such approaches are currently predicated on starting with good crystal structures that closely represent those found under the solution conditions to be simulated. A33 Fab, is an undeveloped immunotherapeutic antibody candidate that was targeted to the human A33 antigen homogeneously expressed in 95% cases of primary and metastatic colorectal cancers. It is now used as a very well characterised testing ground for developing analytics, formulation and protein engineering strategies, and to gain a deeper understanding of mechanisms of destabilisation, representative of the wider therapeutic Fab platform. In this article, we report the structure of A33 Fab in two different crystal forms obtained at widely differing pH. These reveal important insights into the structural impact of pH on the ‘grafted’ complementarity-determining regions (CDRs) and the switch linker between the variable and the constant regions. It also enabled a direct evaluation of the impact of initial crystal structure selection on the outcomes of molecular dynamics simulations under different conditions, and their subsequent use for determining best fit solution structures using previously obtained small-angle x-ray scattering (SAXS) data. The differences in the two structures did not have a major impact on MD simulations regardless of the pH, other than a slight persistence of structure affecting the solvent accessibility of one of the predicted APR regions of A33 Fab. Interestingly, despite being obtained at pH 4 and pH 9, the two crystal structures were more similar to the SAXS solution structures obtained at pH 7, than to those at pH 4 or pH 9. Furthermore, the P65 crystal structure from pH 9 was also a better representation of the solution structures at any other pH, than was the P1 structure obtained at pH 4. Thus, while obtained at different pH, the two crystal structures may represent highly (P65) and lesser (P1) populated species that both exist at pH 7 in solution. These results now lay the foundation for confident MD simulations of A33 Fab that rationalise or predict behaviours in a range of conditions.
Immune-related cystitis due to immune checkpoint inhibitors: a case report
Authors: Li WenLai , Shi Kezhi , Li Xuanwei , Li Yue , Ma Congyuan , Zhu Ping
Published: April 2023

Background：Immune checkpoint inhibitors (ICIs) have been proven to be beneficial in multiple advanced malignancies. However, the widespread use of ICIs also occurred with various immune-related adverse events (irAEs). However, while various immune-related adverse events related to immune checkpoint inhibitors have been reported, there are few reports of lower urinary tract symptoms.
Case presentation：A 42-year-old woman with lung cancer who was being treated with sintilimab, anlotinib, and denosumab presented to the nephrology department with frequent micturition, urgency, odynuria and gross hematuria. Initial laboratory tests did not reveal bacteria, but CT examination suggested cystitis. After empiric antiinfective therapy, the above symptoms did not improve significantly. The patient stopped taking sinlizumab and completed cystoscopy. Based on the combined clinical manifestations and laboratory findings, he was diagnosed with immune-related cystitis. Symptomatic relief was achieved via steroid treatment. Thereafter, the patient has been followed for 3 months without any symptoms or recurrence of immune-related cystitis
Conclusions: immune-related cystitis is a commonly misdiagnosed disease. However, early diagnosis, treatment and prophylaxis through accumulated clinical data can help patients achieve a good prognosis. Therefore, clinicians need to be well aware of the variety of clinical characteristics and treatment options of this disease.
Cross-continental environmental and genome-wide
association study on children and adolescent anxiety
and depression.
Authors: Thapaliya Bishal , Ray Bhaskar , Farahdel Britny , Suresh Pranav , Sapkota Ram , Holla Bharath , Mahadevan Jayant , Chen Jiayu , Vaidya Nilakshi , Perrone-Bizzozero Nora , Benegal Vivek , Schumann Gunter , Calhoun Vince , Liu Jingyu
Published: April 2023

Anxiety and depression in children and adolescents warrant special attention as a public health issue given their devastating
and long-term effects on development and mental health. Multiple factors, ranging from genetic vulnerabilities to environmental
stressors, influence the risk for the disorders. This study investigated the impact of environmental factors and genomics on
anxiety and depression in children and adolescents across three cohorts: the Adolescent Brain and Cognitive Development
Study (US), the Consortium on Vulnerability to Externalizing Disorders and Addictions (India), and IMAGEN (Europe). Linear
mixed-effect models, recursive feature elimination regression, and LASSO regression models were used to identify the
environmental impact on anxiety/depression. Genome-wide association analyses were then performed for all three cohorts
with consideration of significant environmental effects. The most significant and consistent environmental factors were early
life stress and school risk. A novel SNP, rs79878474 in chr11p15, was identified as the most promising SNP associated with
anxiety and depression. Gene set analysis found significant enrichment in regions of chr11p15 and chr3q26, in the function of
potassium channels and insulin secretion, particularly Kv3, Kir-6.2, SUR potassium channels encoded by the KCNC1, KCNJ11,
and ABCCC8 genes, respectively, in chr11p15. Tissue enrichment analysis showed significant enrichment in the small intestine
and a trend of enrichment in the cerebellum. The study highlights the consistent impact of early life stress and school risk
on anxiety and depression during development and suggests the potential role of mutations in potassium channels and the
cerebellum region. Further investigation is needed to better understand these findings.
Prognostic value of immunogenic cell death genes in bladder cancer
Authors: Li Yongchen , Gao Yukui , Yang Zhen , Wu Yudong
Published: April 2023

Background: The poor prognosis of bladder cancer (BLCA) highlights the need for the development of an effective and accurate prognostic model that can guide its diagnosis and treatment. Accumulating evidence suggest that immunogenic cell death (ICD) genes are involved in the BLCA tumorigenesisand which indicate their promise for the development of novel cell death-targeted treatments.
Methods: We extracted 433 BLCA samples from The Cancer Genome Atlas and screened them with univariate Cox, multivariate, and lasso Cox regression analyses. We identified 19 ICD-related differentially expressed genes (DEGs) and used these to create a risk model. The effectiveness of the model was verified using two independent clinical datasets, GSE13507 and GSE32894, while its predictive ability was analyzed via Kaplan-Meier and receiver operating characteristic curve analyses of survival. Finally, we used decision curve analysis to evaluate the 1-, 3-, and 5-year clinical effectiveness of the prognostic model.
Conclusions: We established a risk prognostic model based on ICD-related DEGs in BLCA. This model demonstrated high predictive accuracy for the overall survival of patients with BLCA, paving the way for an improved understanding of the disease.