bluuebunny/crossref_metadata_2025 huggingface dataset:
Participatory evaluation of municipal obesity prevention clubs in Tehran city: Strengths, challenges, and future direction
Authors: Edalati Sareh , Omidvar Nasrin , Takian Amirhossein , Rasam Farzaneh , Ghodsi Delaram , Majdzadeh Reza
Published: February 2023

Background and aim

Community-based initiatives are important for obesity prevention. This study aimed to evaluate the activities of municipal obesity prevention clubs (OBCs) in Tehran, Iran, using a participatory approach.

Methods

The evaluation team was formed, and the members identified the OBC's strengths, and challenges and provided suggestions for change through a participatory workshop, observation, focus group discussions, reviewing relevant documents (n = 97), and 35 interviews with involved stakeholders. The MAXQDA software was used for data analysis.

Results

An empowerment training program for volunteers was identified as one of the strengths of OBCs. Despite the obesity prevention efforts of OBCs through public exercise sessions, healthy food festivals, and educational sessions, several challenges were identified that hinder participation in OBCs. These challenges included poor marketing strategies, poor training approaches in participatory planning, insufficient motivational support for volunteers, low perceived recognition of volunteers by the community, volunteers' low food and nutrition literacy, poor educational services in the communities, and limited funding for health promotion activities.

Conclusion

Weaknesses in all stages of community participation, including information, consultation, collaboration, and empowerment, in OBCs were detected. Facilitating a more enabling environment for informing and involving citizens, expanding neighborhood social capital, and involving health volunteers, academia, and all potential governmental sectors to collaborate for obesity prevention is recommended.
The prevalence of osteoporosis in China, a community based cohort study of osteoporosis
Authors: Wang Jing , Shu Bing , Tang De-zhi , Li Chen-guang , Xie Xing-wen , Jiang Li-juan , Jiang Xiao-bing , Chen Bo-lai , Lin Xin-chao , Wei Xu , Leng Xiang-yang , Liao Zhang-yu , Li Bao-lin , Zhang Yan , Cui Xue-jun , Zhang Qing , Lu Sheng , Shi Qi , Wang Yong-jun
Published: February 2023

Background

Osteoporosis has already been a growing health concern worldwide. The influence of living area, lifestyle, socioeconomic, and medical conditions on the occurrence of osteoporosis in the middle-aged and elderly people in China has not been fully addressed.

Methods

The study was a multicenter cross-sectional study on the middle-aged and elderly permanent residents, which gathered information of 22,081 residents from June 2015 to August 2021 in seven representative regions of China. The bone mineral density of lumbar vertebrae and hip were determined using the dual-energy X-ray absorptiometry densitometer instruments. Serum levels of bone metabolism markers were also measured. Information about education, smoking, and chronic diseases were also collected through face-to-face interviews. Age-standardized prevalence and 95% confidence intervals (CIs) of osteopenia and osteoporosis by various criteria were estimated by subgroups and overall based on the data of China 2010 census. The relationships between the osteoporosis or osteopenia and sociodemographic variables or other factors were examined using univariate linear models and multivariable multinomial logit analyses.

Results

After screening, 19,848 participants (90%) were enrolled for the final analysis. The age-standardized prevalence of osteoporosis was estimated to be 33.49%(95%CI, 32.80–34.18%) in the middle-aged and elderly Chinese permanent residents, for men and women was 20.73% (95% CI, 19.58–21.87%) and 38.05% (95% CI, 37.22–38.89%), respectively. The serum concentrations of bone metabolic markers, and calcium and phosphorus metabolism were influenced by age, body mass index (BMI), gender, education level, regions, and bone mass status. Women, aged 60 or above, BMI lower than 18.5 kg/m2, low education level including middle school, primary school and no formal education as well as current regular smoking, a history of fracture were all significantly associated with a higher risk of osteoporosis and osteopenia in the middle-aged and elderly people.

Conclusions

This study revealed dramatic regional differences in osteoporosis prevalence in China, and female, aged 60 or older, low BMI, low education level, current regular smoking, and a history of fracture were associated with a high risk of osteoporosis. More prevention and treatment resources should be invested into particular population exposed to these risk factors.
Implementation of UNICEF and WHO's care for child development package: Lessons from a global review and key informant interviews
Authors: Ahun Marilyn N. , Aboud Frances , Wamboldt Claire , Yousafzai Aisha K.
Published: February 2023

Introduction

In the last decade, there has been increased global policy and program momentum to promote early childhood development. The Care for Child Development (CCD) package, developed by UNICEF and the WHO, is a key tool responding to the global demand. The CCD package comprises two age-specific evidence-based recommendations for caregivers to 1) play and communicate and 2) responsively interact with their children (0–5 years) and was designed to be integrated within existing services to strengthen nurturing care for child development. The aim of this report was to provide an up-to-date global review of the implementation and evaluation of the CCD package.

Methods

In addition to a systematic review of 55 reports, we interviewed 23 key informants (including UNICEF and WHO personnel) to better understand the implementation of CCD.

Results

The CCD package has been or is being implemented in 54 low- and middle-income countries and territories, and it has been integrated into government services across the health, social, and education sectors in 26 countries. Across these contexts, CCD has been adapted in three primary ways: 1) translations of CCD materials (mostly counseling cards) into local language(s), 2) adaptations of CCD materials for the local context, vulnerable children, or a humanitarian/emergency setting (e.g., including local play activities, using activities that are better suited to children with visual impairments), and 3) substantive modifications to the content of CCD materials (e.g., expansion of play and communication activities, addition of new themes, creation of a structured curriculum). While there is promising evidence and examples of good implementation practice, there has been mixed experience about implementation of CCD with respect to adaptation, training, supervision, integration into existing services, and monitoring implementation fidelity and quality. For example, many users of CCD found difficulties with training the workforce, garnering buy-in from governments, and ensuring benefits for families, among others.

Discussion

Additional knowledge on how to improve the effectiveness, implementation fidelity and quality, and acceptance of CCD is needed. Based on the findings of the review we make recommendations for future efforts to implement CCD at-scale.
Argument-based human–AI collaboration for supporting behavior change to improve health
Authors: Kilic Kaan , Weck Saskia , Kampik Timotheus , Lindgren Helena
Published: February 2023

This article presents an empirical requirement elicitation study for an argumentation-based digital companion for supporting behavior change, whose ultimate goal is the promotion and facilitation of healthy behavior. The study was conducted with non-expert users as well as with health experts and was in part supported by the development of prototypes. It focuses on human-centric aspects, in particular user motivations, as well as on expectations and perceptions regarding the role and interaction behavior of a digital companion. Based on the results of the study, a framework for person tailoring the agent's roles and behaviors, and argumentation schemes are proposed. The results indicate that the extent to which a digital companion argumentatively challenges or supports a user's attitudes and chosen behavior and how assertive and provocative the companion is may have a substantial and individualized effect on user acceptance, as well as on the effects of interacting with the digital companion. More broadly, the results shed some initial light on the perception of users and domain experts of “soft,” meta-level aspects of argumentative dialogue, indicating potential for future research.
Neural networks to retrieve in water constituents applied to radiative transfer models simulating coastal water conditions
Authors: Hadjal Madjid , Paterson Ross , McKee David
Published: February 2023

Estimation of chlorophyll (CHL) using ocean colour remote sensing (OCRS) signals in coastal waters is difficult due to the presence of two other constituents altering the light signal: coloured dissolved organic material (CDOM) and mineral suspended sediments (MSS). Artificial neural networks (NNs) have the capacity to deal with signal complexity and are a potential solution to the problem. Here NNs are developed to operate on two datasets replicating MODIS Aqua bands simulated using Hydrolight 5.2. Artificial noise is added to the simulated signal to improve realism. Both datasets use the same ranges of in water constituent concentrations, and differ by the type of logarithmic concentration distributions. The first uses a Gaussian distribution to simulate samples from natural water conditions. The second uses a flat distribution and is intended to allow exploration of the impact of undersampling extremes at both high and low concentrations in the Gaussian distribution. The impact of the concentration distribution structure is assessed and no benefits were found by switching to a flat distribution. The normal distribution performs better because it reduces the number of low concentration samples that are relatively difficult to resolve against varying concentrations of other constituents. In this simulated environment NNs have the capacity to estimate CHL with outstanding performance compared to real in situ algorithms, except for low values when other constituents dominate the light signal in coastal waters. CDOM and MSS can also be predicted with very high accuracies using NNs. It is found that simultaneous retrieval of all three constituents using multitask learning (MTL) does not provide any advantage over single parameter retrievals. Finally it is found that increasing the number of wavebands generally improves NN performance, though there appear to be diminishing returns beyond ∼8 bands. It is also shown that a smaller number of carefully selected bands performs better than a uniformly distributed band set of the same size. These results provide useful insight into future performance for NNs using hyperspectral satellite sensors and highlight specific wavebands benefits.
Small fiber polyneuropathy: A new therapeutic target in patients with interstitial cystitis/bladder pain syndrome?
Authors: Whitman Wyatt , Sandberg Maxwell , Lee Peyton , Walker Stephen J.
Published: February 2023

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating chronic disease that, based on the variable efficacy observed with most therapeutic options, is difficult to treat effectively. A more targeted patient selection process for current and emerging therapeutic options would likely help to improve outcomes. This narrative review explores small fiber polyneuropathy (SFPN) in IC/BPS as part of a larger widespread pain phenotype and as a potential therapeutic target. Because SFPN is becoming increasingly implicated in polysyndromic pain disorders (e.g., IC/BPS, chronic pelvic pain, and fibromyalgia) in which immune dysregulation is a suspected pathophysiologic etiology, continued consideration should be given to immunomodulatory therapies such as intravenous immunoglobulin (IVIg). Moreover, since the small fibers affected in SFPN continue to grow even as people age, targeted treatment may prevent further destruction and provide long-term benefits as the fibers are given time to repair. In addition to therapeutic potential, having a definitive SFPN diagnosis may provide psychological benefit in a patient population for which symptoms have historically been attributed to negative psychological factors. Finally, based on emerging data in this area, we propose consideration be given to include SFPN testing in the work-up of patients with IC/BPS that are refractory to treatments or have multiple comorbid pain syndromes since it may be an indicator of the need for alternative therapies. We believe that SFPN will play an increasingly larger role in the clinical evaluation and management of polysyndromic pain disorders, including IC/BPS.
Ground-based infrastructure for monitoring and characterizing intermediate-scale ionospheric irregularities at mid-latitudes
Authors: Mrak Sebastijan , Coster Anthea J. , Groves Keith , Nikoukar Romina
Published: February 2023

We discuss potential science investigations at mid-latitudes enabled by a modern, space-weather-grade, ground-based Radio-Frequency network of scintillation receivers which encompasses Global Navigation Satellite Systems and Beacon receivers, along with coherent radars, and leveraging radio astronomy infrastructure for space weather application. The primary scientific research addresses the controlling space weather drivers for the structuring of mid-latitude ionospheric plasma at intermediate scales (10s of meters—10s of kilometers), their relationship with larger density structures, and their impacts on the trans-ionospheric radio links. These irregularities scintillate the signals impairing the radio link integrity and the underpinning services. The suggested science investigations are currently unable to be fully accomplished because of missing high-fidelity and long-term observations at satisfactory spatial coverage. We discuss the physics responsible for the radio wave disturbances and their impacts, review the current state of knowledge based on available observations, and outline a plan for developing the necessary infrastructure by leveraging existing ground-based distributed observatories that will enable novel scientific investigations and will be synergistic with other geoscience divisions such as seismology, geology, and meteorology.
Cultured meat, clean meat,… queer meat? A vegan queer ecofeminist perspective on the implications of cellular agriculture
Authors: Jiménez Rodríguez Adriana
Published: February 2023

This perspective article briefly explores the social implications of cellular agriculture from a Vegan Queer Ecofeminist point of view by referring to a synthesis of currently agreed-upon (possible) positive and negative effects of post-animal agriculture and highlighting how these effects actually ignore key ethical problems inherent in animal agriculture itself. By invisibilizing these, discussions of cellular-ag remain in danger of obscuring the ways in which an intact and unexamined paradigm based on capitalist, patriarchal speciesism will continue to foment exploitative and unjust practices in a dying planet. The article emphasizes the urgent need to address the complexities of cellular-ag from a multidisciplinary perspective that actively engages with the demands of true global justice for all, nonhuman and human.
Case report: Suspected plasmablastic lymphoma in a dog resembling the clinical presentation in humans
Authors: Giuliano Antonio , Lam Sin Yan , Flecher Mayra C. , Horta Rodrigo S.
Published: February 2023

Plasmablastic lymphoma (PBL) is a rare form of lymphoma in people. PBL originates from plasmablasts and usually presents with swelling/mass in the mouth/neck. A 7-year-old Mongrel dog was presented for a large oral and neck mass. Cytology and histopathology were suggestive of a round cell tumor that was suspected to be lymphoma. An immunohistochemical (IHC) stain panel showed positive for CD18, thus supporting the diagnosis of round cell tumor, but negative for T- and B-cell lymphomas, CD3, CD20, and PAX-5. Other markers including cytokeratin AE1/3 (for epithelial cell origin), CD31 (for endothelial cells), SOX10 (for melanoma), IBa-1 (for histiocytic sarcoma), and CD117 (for mast cell tumor) were all negative. MUM-1 (for plasma cell differentiation) was strongly positive and CD79a (B cell and plasma cells) was also scantly positive. Based on the histopathology and immunohistochemistry results in combination with the clinical presentation, a suspected diagnosis of PBL was made. As per available literature, this is perhaps the first highly suspected case of PBL in a dog.
Distinct differences in immunological properties of equine orthobiologics revealed by functional and transcriptomic analysis using an activated macrophage readout system
Authors: Pezzanite Lynn M. , Chow Lyndah , Griffenhagen Gregg M. , Bass Luke , Goodrich Laurie R. , Impastato Renata , Dow Steven
Published: February 2023

Introduction

Multiple biological therapies for orthopedic injuries are marketed to veterinarians, despite a lack of rigorous comparative biological activity data to guide informed decisions in selecting a most effective compound. Therefore, the goal of this study was to use relevant bioassay systems to directly compare the anti-inflammatory and immunomodulatory activity of three commonly used orthobiological therapies (OTs): mesenchymal stromal cells (MSC), autologous conditioned serum (ACS), and platelet rich plasma (PRP).

Methods

Equine monocyte-derived macrophages were used as the readout system to compare therapies, including cytokine production and transcriptomic responses. Macrophages were stimulated with IL-1ß and treated 24 h with OTs, washed and cultured an additional 24 h to generate supernatants. Secreted cytokines were measured by multiplex immunoassay and ELISA. To assess global transcriptomic responses to treatments, RNA was extracted from macrophages and subjected to full RNA sequencing, using an Illumina-based platform. Data analysis included comparison of differentially expressed genes and pathway analysis in treated vs. untreated macrophages.

Results

All treatments reduced production of IL-1ß by macrophages. Secretion of IL-10 was highest in MSC-CM treated macrophages, while PRP lysate and ACS resulted in greater downregulation of IL-6 and IP-10. Transcriptomic analysis revealed that ACS triggered multiple inflammatory response pathways in macrophages based on GSEA, while MSC generated significant downregulation of inflammatory pathways, and PRP lysate induced a mixed immune response profile. Key downregulated genes in MSC-treated cultures included type 1 and type 2 interferon response, TNF-α and IL-6. PRP lysate cultures demonstrated downregulation of inflammation-related genes IL-1RA, SLAMF9, ENSECAG00000022247 but concurrent upregulation of TNF-α, IL-2 signaling, and Myc targets. ACS induced upregulation of inflammatory IL-2 signaling, TNFα and KRAS signaling and hypoxia, but downregulation of MTOR signaling and type 1 interferon signaling.

Discussion

These findings, representing the first comprehensive look at immune response pathways for popular equine OTs, reveal distinct differences between therapies. These studies address a critical gap in our understanding of the relative immunomodulatory properties of regenerative therapies commonly used in equine practice to treat musculoskeletal disease and will serve as a platform from which further in vivo comparisons may build.
Numerical Study on Unbalance Response of Dual-Rotor System Based on Nonlinear Bearing Characteristics of Active Magnetic Bearings
Authors: Wang Nianxian , Liu Mingzheng , Yao Junfu , Ge Pingping , Wu Huachun
Published: February 2023

The magnetic suspended dual-rotor system (MSDS) has the advantage of a high power density. The system can be used in high-speed rotating machinery. The major purpose of this study is to predict the unbalance response of the MSDS considering the nonlinear bearing characteristics of active magnetic bearings (AMBs). Firstly, the nonlinear bearing model was established by a non-linear magnetic circuit method (NMCM). The model considers magnetic flux leakage, magnetic saturation, and working position flotation accurately. Then, the dynamic model of the system was established by using the finite element method and solved by the Newmark-β method. Finally, the effects of external load, rotational speeds, and control parameters were studied. Axial trajectory diagrams, stability zone diagrams, and waterfall diagrams were employed to analyze the dynamic behaviors of the MSDS. The results indicate that the external load, rotational speeds, and control parameters have a significant impact on the unbalance response of the system. Super harmonics of rotational frequencies and their combined frequencies may be excited by heavy load conditions. Appropriate control parameters can suppress the nonlinear phenomena. The obtained results of this research will contribute to the design and fault diagnosis of MSDSs.
On the Self-Similarity in an Annular Isolator under Rotating Feedback Pressure Perturbations
Authors: Luo Zhongqi , Huang Hexia , Tan Huijun , Liang Gang , Lv Jinghao , Wu Yuwen , Li Liugang
Published: February 2023

In this paper, the transient flow simulation in an annular isolator under rotating feedback pressure perturbations simplified from the rotating denotation wave (RDW) is performed. The instantaneous flow characteristics and the self-similarity of the isolator flow-field are investigated in detail. It is found that a helical moving shock wave (MSW) and a quasi-toroidal terminal shock wave (TSW) are induced in the isolator. Hence, the flow-fields on the meridian planes could be classified into three zones, i.e., the undisturbed zone, the terminal shock wave/moving shock wave/boundary layer interaction (TSW/MSW/BLI) zone and the moving shock wave/boundary layer interaction (MSW/BLI) zone. The TSW/MSW/BLI zone is characterized by the coupling of the TSW/BLI and the MSW/BLI due to their small axial distance, which intensifies the adverse pressure gradient on the meridian planes, thus rolling up large separation bubbles developing along the MSW driven by the circular pressure gradient. In the MSW/BLI zone, the shock induces the boundary layer to separate, forming a helical vortex located at the foot of the MSW. During the upstream propagation process, the pattern of the MSWs transforms from a moving normal shock wave to a moving oblique shock wave with decreased strength. Moreover, after the collision with the MSWs, P, Temp and S of the flow elevate with the prompt decrease of va, while vθ increases to a higher level. Despite the deflection effect of the MSWs on the streamlines, the flow direction of the air still maintains an almost axial position at the exit, except in the adjacent region of the MSW. Likewise, three types of zones can be determined in the flow pattern at the exit: the rotating detonation wave/boundary layer interaction (RDW/BLI) zone, the expansion zone, and the vortices discharge zone. Comparing the transient flow patterns at different moments in one cycle and between adjacent cycles, an interesting discovery is that the self-similarity property is observed in the flow-field of the annular isolator under rotating feedback pressure perturbations. The global flow structure of the isolator at different moments shows good agreement despite its rotation with the RDW, and the surface pressure profiles of the corresponding meridian planes all match perfectly. Such a characteristic indicates that the rotation angular velocity of the TSW and the MSW are equal and hold invariant, and the isolator flow could be regarded as a quasi-steady flow. On this basis, the theoretical model of the inclination angles of the MSW by the coordinate transformation and velocity decomposition is developed and validated. The relative errors of the inclination angles between the predicted and measured results are below 3%, which offers a rapid method to predict the shape of the MSW, along with a perspective to better understand the physical meaning of the shape of the MSW.
Implementation Framework for Transformation of Peat Ecosystems to Support Food Security
Authors: Hidayat Dian Charity , Mizuno Kosuke , Said Chairil Abdini Abidin , Herdiansyah Herdis
Published: February 2023

The development of food estates, including peatland ecosystems, is expected to support food security in all regions equally. Technically, the widespread clearing of peatlands for cultivating a specific food crop has multiple environmental effects. The need for more attention to non-technical aspects related to the socioeconomic life of the surrounding community is also an obstacle to the sustainability of the food estate program. In support of food security and sovereignty, this study aimed to develop an implementation framework for sustainably utilizing peat ecosystems as arable land-producing food commodities. A systematic literature review in accordance with the PRISMA guidelines and field observations were used to develop ecosystem concepts by focusing on maintaining an ecosystem’s social, economic, and ecological aspects. This paper describes the history of peat ecosystem utilization, evaluates previous errors in peatland clearing for food, and analyzes the data using the perspective of peat hydrological unit to better understand the livelihood preferences of existing communities. The previous literature’s key findings served as the guidelines for constructing the implementation framework. First, this method identifies people’s natural resource-based livelihoods and describes peatland ecosystems. Second, it evaluates the long-term viability of livelihoods and identifies improvement levers. Finally, it facilitates increasing the scale of food commodities produced from sustainable livelihoods to meet market demand while maintaining ecosystem resilience.
Developing an NIRS Prediction Model for Oil, Protein, Amino Acids and Fatty Acids in Amaranth and Buckwheat
Authors: Shruti  , Shukla Alka , Rahman Saman Saim , Suneja Poonam , Yadav Rashmi , Hussain Zakir , Singh Rakesh , Yadav Shiv Kumar , Rana Jai Chand , Yadav Sangita , Bhardwaj Rakesh
Published: February 2023

Amaranth and buckwheat are two pseudo-cereals preferred for their high nutritional value, are gluten free and carry religious importance as fasting food. Germplasm resources are the reservoir of diversity for different traits, including nutritional characteristics. These resources must be evaluated to utilize their potential in crop improvement programs. However, conventional methods are labor-, cost- and time-intensive and prone to handling errors when applied to large samples. NIRS-based machine learning to predict different nutritional traits is applied in different food crops for multiple traits. NIRS prediction models are developed in this study using the mPLS regression technique for oil, protein, fatty acids and essential amino acid estimation in amaranth and buckwheat. Good RSQ external (power of determination) values were obtained for the above traits ranging from 0.72 to 0.929. Ratio performance deviation (RPD) value for most of the traits ranged between 2 and 3, except for valine (1.88) and methionine (3.55), indicating good prediction capabilities in the developed model. These prediction models were utilized in screening the germplasm of amaranth and buckwheat; the results obtained were in good agreement and confirmed the applicability of developed models. It will enable the identification of a trait-specific germplasm as a potential gene source and aid in crop improvement programs.
Evaluation of Biostimulatory Activity of Commercial Formulations on Three Varieties of Chickpea
Authors: Gabilondo Regina , Sánchez Jorge , Muñoz Pedro , Montero-Muñoz Iris , Mauri Pedro V. , Marín José , Mostaza-Colado David
Published: February 2023

Biostimulants are studied as a possible agricultural practice that anticipates the reproductive stages of chickpeas to avoid their coincidence with high temperatures and hydric stress periods. The effect of several types of biostimulants on different chickpea varieties was analyzed. The Blanco Sinaloa chickpea variety showed opposite patterns with respect to biostimulant effect on germination success and vegetative and radicular development when compared with two other chickpea varieties, namely Amelia, a well-known variety, and IMIDRA10, a recently developed variety. Blanco Sinaloa is cultured under water irrigation conditions, while Amelia and IMIDRA10 are used under rainfed conditions. Blanco Sinaloa and IMIDRA10 are Kabuli-type varieties, while Amelia is Desi-type. All varieties emerged 9 days after the sowing, but Amelia nascence was more abundant at the beginning, on day 9. On day 32, the picture was quite different, since Blanco Sinaloa had germinated 100% in practically all treatments, followed by Amelia and IMIDRA10. There were significant differences between plant lengths among the three varieties, since Blanco Sinaloa is much larger than Amelia and IMIDRA10. Blanco Sinaloa was the only variety in which the plant lengths of biostimulant-impregnated seeds were superior to those of untreated plants; that is, it was the only one that was positively affected by biostimulants. Chickpea seeds should be treated with biostimulants such that they are dry for sowing, because the mechanic seeder only works with dry seeds.
Biogeneration of Valuable Nanomaterials from Agro-Wastes: A Comprehensive Review
Authors: Nath Pinku Chandra , Ojha Amiya , Debnath Shubhankar , Sharma Minaxi , Sridhar Kandi , Nayak Prakash Kumar , Inbaraj Baskaran Stephen
Published: February 2023

The exponential growth of agricultural output is a result of the increasing world population. Agro-wastes are now acknowledged as an alternative material for the manufacture of renewable, inexpensive, and sustainable bio-composite-based products. Huge amounts of agricultural produce are often lost owing to a lack of processing facilities or storage space. Agriculture wastes contain a significant concentration of carbohydrates as well as various multifunctional groups and organic substances, such as polymeric proteins. Interestingly, nanotechnology provides better potential to transform agricultural wastes easily into valuable and cost-effective products, removing the need to utilize noxious chemicals, which can create a variety of health and environmental difficulties. Recently, there has been an increase in interest in eco-friendly nanomaterial (NM) production techniques that utilize extracts generated from agricultural waste. Such nanoparticles (NPs) have been extensively studied for numerous uses, including antibacterial agents, water purification, the breakdown of industrial colours, and many others. Consequently, the purpose of this review is to investigate the different sources, characteristics, and nano-management of agro-waste; valuable NMs derived from agro-waste; and their possible applications.
Effect of L-Tryptophan and L-Glutamic Acid on Carrot Yield and Its Quality
Authors: Rosa Robert , Hajko Larysa , Franczuk Jolanta , Zaniewicz-Bajkowska Anna , Andrejiová Alena , Mezeyová Ivana
Published: February 2023

Positively affecting crop quality and yields, amino acids used as plant stimulants play a special role in ensuring global food security. L-tryptophan (L-Try) and L-glutamic acid (L-Glu) are important biostimulants that increase the yield of field crops and vegetables. Carrot is one of the most important vegetables due to its production volume in the world (sixth most consumed vegetable) and its nutritional value. The response of different plant species to amino acid application varies. The literature mainly deals with the effects of ready-made products containing a mixture of several amino acids, with no exhaustive studies on the effects of individual amino acids on carrot quality and yield. This paper is based on a two-year field experiment (2019–2020), in which the effect of two amino acids, L-Trp (7.5 g·ha−1) and L-Glu (60.0 g·ha−1), on carrot (Daucus carota L.) was investigated. They were applied to the leaves (FA) or both to the soil and to the leaves (S + FA), separately, (L-Trp or L-Glu) or as a mixture (L-Trp + L-Glu). The control plot was treated with mineral fertilizers only. The research was conducted as a field experiment in a split-block design. The yield of carrot storage roots and their content of dry matter, protein, sugars, total soluble solids (TSS), and ascorbic acid were determined. The amino acids positively affected the yield of carrots compared to the control, but only the synergistic action of L-tryptophan and L-glutamic acid increased it significantly. On average, for both amino acids the S + FA application increased the protein content and the marketable yield of storage roots significantly more than in response to FA treatment. A significant increase in marketable yield compared to the control was found after the combined soil and foliar application (S + FA) of all amino acid combinations, but the L-Trp + L-Glu mixture worked best. The storage roots of carrots grown on the plot with L-Trp + L-Glu contained significantly more protein and TSS than those on the control plot. The content of TSS was also positively affected by L-Trp used on its own, while L-Glu increased the content of ascorbic acid. Amino acids applied to the leaves (FA) increased the content of total sugars in the carrot roots more than when applied both to the soil and to the leaves (S + FA). Of all treatment combinations, the synergistic action of L-Trp and L-Glu made it possible to obtain the highest yields of carrot storage roots, containing the most protein and total soluble solids.
Environmental DNA Assay for the Detection of the American Bullfrog (Lithobates catesbeianus) in the Early Stages of the Invasion in the Ebre Delta
Authors: Sanz Nuria , Franch Nati , Araguas Rosa-Maria , Viñas Jordi , Vidal Oriol
Published: February 2023

The American bullfrog (Lithobates catesbeianus) is considered to be one of the most harmful invasive species. In the Iberian Peninsula, this species had been cited occasionally until the year 2018, when L. catesbeianus appeared in the Ebre Delta, and, for the first time, it started breeding in a territory of the Peninsula. Using environmental DNA (eDNA) analysis and visual surveys, the American bullfrog invasion in the Ebre Delta was monitored across two consecutive years (2019–2020). No specimens were observed in 2019, and results for the eDNA survey also failed to detect this species in the Delta. In 2020, two individuals were captured and, under the most conservative criteria to constrain the number of positive detections, eDNA analyses detected the presence of the American bullfrog in at least five locations. Performing an eDNA assay yielded a higher sensitivity with a lower sampling effort than traditional methods. Although the American bullfrog does not appear to still be well-established in the Ebre Delta, only a few bullfrog individuals could be enough for their establishment in suitable habitats. In this context, eDNA assays are essential tools to facilitate the detection, control, and eradication of this species in the first stage of the invasion process.
Temporal Response of Mesocarnivores to Human Activity and Infrastructure in Taihang Mountains, Central North China: Shifts in Activity Patterns and Their Overlap
Authors: Chen Yanzhi , Liu Beibei , Fan Deqing , Li Sheng
Published: February 2023

Mesocarnivores play essential roles in terrestrial ecosystems, but anthropocentric disturbances have profoundly transformed their intraguild interactions worldwide. In this study, we explored how a guild of four mesocarnivores (red fox Vulpes vulpes, leopard cat Prionailurus bengalensis, Asian badger Meles leucurus, and hog badger Arctonyx collaris) partition their temporal niche in the temperate montane forests in North China under different human influences. We conducted a systemic camera-trapping survey on the study species in the central Taihang Mountains from 2016 to 2020. With an extensive survey effort of 111,063 camera-days from 187 camera stations, we obtained 10,035 independent detections of the four mesocarnivores and examined the activity patterns of each species under different levels of human disturbance and their overlaps. The results showed that, while the leopard cat and the badgers shifted their activity towards nocturnality, the red fox showed no significant change. The leopard cat’s degree of nocturnality varied between growing and non-growing seasons, likely a response to avoid humans and other competitors. However, the activity overlaps between species pairs demonstrated no statistically significant difference, indicating a long-developed coexistence mechanism that is homogenous across the landscape. Demonstrating how mesocarnivores shift activity patterns in response to human risks while partitioning resources, this study enhances our understanding of mesocarnivore behavioral changes and interspecific interactions at human–nature interfaces.
Putative Role of CFSH in the Eyestalk-AG-Testicular Endocrine Axis of the Swimming Crab Portunus trituberculatus
Authors: Wang Mengen , Xu Rui , Tu Shisheng , Yu Qiaoling , Xie Xi , Zhu Dongfa
Published: February 2023

It has been shown in recent studies that the crustacean female sex hormone (CFSH) plays a crucial role in the development of secondary sexual characteristics in Decapoda crustaceans. However, research on the function of CFSH in the eyestalk-AG-testicular endocrine axis has been inadequate. We cloned and identified a homolog of CFSH, PtCFSH, in this study. RT-PCR showed that PtCFSH was mainly expressed in the eyestalk. A long-term injection of dsPtCFSH and recombinant PtCFSH (rPtCFSH) in vivo showed opposite effects on spermatogenesis-related gene expression and histological features in the testis of P. trituberculatus, and was accompanied by changes in AG morphological characteristics and PtIAG expression. In addition, the phosphorylated-MAPK levels and the expression of several IIS pathway genes in the testis was changed accordingly in two treatments, suggesting that PtCFSH may regulate the testicular development via IAG. The hypothesis was further validated by a mixed injection of both dsPtCFSH and dsPtIAG in vivo. The following in vitro studies confirmed the negatively effects of PtCFSH on AG, and revealed that the PtCFSH can also act directly on the testis. Treatment with rPtCFSH reduced the cAMP and cGMP levels as well as the nitric oxide synthetase activity. These findings provide vital clues to the mechanisms of CFSH action in both the eyestalk-AG-testis endocrinal axis and its direct effects on the testis.
Antibiotic Drug Resistance Pattern of Uropathogens in Pediatric Patients in Pakistani Population
Authors: Iqbal Zakia , Sheikh Ahsan Sattar , Basheer Anwaar , Hafsa Hadiqa tul , Ahmed Mehboob , Sabri Anjum Nasim , Shahid Samiah
Published: February 2023

The common prevalent diseases in the age of 0 to 6 are related to urinary tract infections. If not properly diagnosed, they will lead to urological and nephrological complications. Uropathogens are developing resistance against most drugs and are harder to treat. A study was done on the inpatients and outpatients of the two hospitals located in Lahore. A total of 39,750 samples that were both male and female were collected. Escherichia and Klebsiella were found in 234 samples based on biochemical characterization, growth on CLED agar, and white blood cell/pus cell (WBC) microscopy. In comparison to males, female samples had a higher number of uropathogens (1:1.29). From the samples of Shaikh Zayed Hospital (SZH), the ratio of Klebsiella to Escherichia (1:1.93) was reported, while this ratio was 1.84:1 from the Children Hospital (CH). The incidence of UTI was higher in the month of September. Randomly selected Escherichia and Klebsiella were verified via a 16S rRNA sequence. Antibiotic resistance profiling of isolated bacterial strains was done against 23 antibiotics. The most efficient antibiotics against Klebsiella and Escherichia were colistin sulphate (100% sensitivity against bacteria from CH; 99.3% against strains from SZH) and polymyxin B (100% sensitivity against strains from SZH; 98.8% against strains from CH). Sensitivity of the total tested strains against meropenem (74%, SZH; 70% CH), Fosfomycin (68%, SZH; 73% CH strains), amikacin (74% SZH; 55% CH), and nitrofurantoin (71% SZH;67% CH) was found, Amoxicillin, ampicillin, and cefuroxime showed 100 to ≥90% resistance and are the least effective.
Schizochytrium spp. Dietary Supplementation Modulates Immune-Oxidative Transcriptional Signatures in Monocytes and Neutrophils of Dairy Goats
Authors: Kyriakaki Panagiota , Mavrommatis Alexandros , Tsiplakou Eleni
Published: February 2023

The high propensity of dietary polyunsaturated fatty acids (PUFA) to oxidation can induce a cascade of cellular immune-oxidative imbalances. On the other hand, PUFA, namely docosapentaenoic acid (ω6-DPA) and docosahexaenoic acid (DHA) can exert immunomodulatory effects by suppressing a pro-inflammatory response. Thus, the objective of this study was to investigate the effect of dietary supplementation with Schizochytrium spp. levels, rich in both ω6-DPA and DHA on the transcriptional profiling of genes involved in oxidative homeostasis and innate immunity of dairy goats’ monocytes and neutrophils. Twenty-four dairy goats were divided into four homogeneous sub-groups; the diet of the control group (CON) had no Schizochytrium spp. while those of the treated groups were supplemented daily with 20 (ALG20), 40 (ALG40), and 60 (ALG60) g/goat/day. The mRNA levels of MGST1 in neutrophils were downregulated (p = 0.037), while in monocytes, SOD2 and SOD3 were downregulated (p = 0.010 and p = 0.044, respectively) in ALG60 compared to the CON group. GPX2 mRNA levels were downregulated (p = 0.036) in ALG20 and ALG60 compared to the CON group in neutrophils. NOX1 was upregulated (p = 0.043) in the neutrophiles of ALG60-goats. NOX2 was upregulated (p = 0.042) in the monocytes of ALG40-fed goats, while higher (p = 0.045) levels were also found in the ALG60 group in neutrophils. The mRNA levels of COX2 were downregulated (p = 0.035) in monocytes of the ALG40 and ALG60 groups. The mRNA levels of PTGER2 were also downregulated (p = 0.004) in monocytes of Schizochytrium-fed goats, while in neutrophils, significant downregulation (p = 0.024) was only found for ALG60 compared to the CON group. ALOX5AP mRNA levels were significantly decreased (p = 0.033) in ALG60 compared to the CON group in monocytes. LTA4H mRNA levels were increased (p = 0.015) in ALG60 compared to ALG20 and ALG40 groups in monocytes, while in neutrophils, a significant downregulation (p = 0.028) was observed in ALG20 compared to the CON group. The inclusion of more than 20 g Schizochytrium spp./day in goats’ diet induced imbalances in mechanisms that regulate the antioxidant system, while downregulated the expression of pro-inflammatory pathways in monocytes and neutrophils.
Development of Dairy Products Fortified with Plant Extracts: Antioxidant and Phenolic Content Characterization
Authors: Kandyliari Aikaterini , Potsaki Panagiota , Bousdouni Panoraia , Kaloteraki Chrysoula , Christofilea Martha , Almpounioti Kalliopi , Moutsou Andreani , Fasoulis Chistodoulos K. , Polychronis Leandros V. , Gkalpinos Vasileios K. , Tzakos Andreas G. , Koutelidakis Antonios E.
Published: February 2023

In recent decades, there has been growing interest in the fortification of dairy products with antioxidants and phenolics derived from plant byproducts and herbs. The present study focused on the analysis of dairy products, including kefir, cream cheese, yogurt, and vegan yogurt, enhanced with aqueous extracts of plant byproducts (Citrus aurantium peel, Citrus limon peel and Rosa canina seed) and herbs (Sideritis spp., Hypericum perforatum, Origanum dictamnus, Mentha pulegium L., Melissa oficinallis, Mentha spicata L. and Lavandula angustifolia) to characterize their antioxidant content, phenolic profile, and organoleptic characteristics. Antioxidant and phenolic content were determined by Folin–Ciocalteu and ferric reducing antioxidant power (FRAP) assays and presented values up to 46.61 ± 7.22 mmol Fe2+/L and 82.97 ± 4.29 mg gallic acid (GAE)/g, respectively for the aqueous extracts, as well as up to 0.68 ± 0.06 mmol Fe2+/L and 2.82 ± 0.36 mg GAE/g for the fortified dairy products. The bioavailability of antioxidants and phenolics in fortified foods was determined after in vitro digestion and ranged between 4 and 68%. The phytochemical profile of the aqueous extracts was determined by mass spectrometry, and 162 phytochemicals were determined, from which 128 belong to the polyphenol family including flavonoids and phenolic acids. Furthermore, most of the identified compounds have been recorded to possess enhanced antioxidant capacity in correlation to the in vitro findings. Finally, organoleptic evaluation showed an overall acceptability around 3.0 ± 1.0 on a 5-point scale. In conclusion, the studied plants and herbal extracts can be used for the fortification of a variety of dairy products with potential positive effects on human health.
Development of the Algorithmic Basis of the FCAZ Method for Earthquake-Prone Area Recognition
Authors: Agayan Sergey M. , Dzeboev Boris A. , Bogoutdinov Shamil R. , Belov Ivan O. , Dzeranov Boris V. , Kamaev Dmitriy A.
Published: February 2023

The present paper continues the series of publications by the authors devoted to solving the problem of recognition regions with potential high seismicity. It is aimed at the development of the mathematical apparatus and the algorithmic base of the FCAZ method, designed for effective recognition of earthquake-prone areas. A detailed description of both the mathematical algorithms included in the FCAZ in its original form and those developed in this paper is given. Using California as an example, it is shown that a significantly developed algorithmic FCAZ base makes it possible to increase the reliability and accuracy of FCAZ recognition. In particular, a number of small zones located at a fairly small distance from each other but having a close “internal” connection are being connected into single large, high-seismicity areas.
The Loop-Mediated Isothermal Amplification for the Rapid Detection of Porphyromonas Gingivalis
Authors: Lenkowski Marcin , Nijakowski Kacper , Woś Patryk , Kaczmarek Mariusz , Surdacka Anna
Published: February 2023

Periodontitis is a highly prevalent oral disease occurring across the globe, resulting from an interaction of many predisposing factors. Our study aimed to determine some chosen periopathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, Tannarella forsythia) using three different methods: loop-mediated isothermal amplification (LAMP), polymerase chain reaction (PCR) and real-time PCR (qPCR), and to compare their sensitivity and specificity. The study included 62 patients with diagnosed periodontitis. Gingival crevicular fluid was collected from all patients to take samples of bacterial DNA for the further laboratory molecular analyses. In comparison to the gold standard (qPCR), the best diagnostic quality parameters were achieved for LAMP using the TE buffer for the P. gingivalis determination. Therefore, the LAMP is an analytical technique that could be used to quickly assess the presence of periopathogens in an outpatient setting.