bluuebunny/crossref_metadata_2025 huggingface dataset:
Dysfunctional Attitudes Mediate the Relationship Between Childhood Emotional Neglect and Anhedonia in Young Adult Major Depression Patients
Authors: Wang Peilin , Zhang Nan , Ma Simeng , Kang Lijun , Wang Wei , Zong Xiaofen , Bai Hanping , Li Ruiting , Liu Zhongchun
Published: January 2022

Background

Childhood traumas are well-established risk factors for major depressive disorder (MDD). However, the relationship between childhood traumas types and MDD symptoms is unclear. The present study tested the hypothesis that childhood traumas affect specific types of anhedonia in depression and the mediating role of dysfunctional attitude.

Methods

Within this cross-sectional study, 310 young adult patients with MDD completed the PHQ-9, CTQ-SF, DAS, and SHAPS. The statistical analyses used the Mann-Whitney U test, Spearman's rank correlation, and multiple regression analysis. Mediation analyses were tested by the structural equation model (SEM).

Results

Spearman's rank correlation analysis showed positive correlations between the SHAPS, CTQ-SF, and DAS total score (p < 0.05). The EA, EN, PN, and SHAPS scores were positively correlated (p < 0.05). Among the four factors of anhedonia, social interaction and interest/pastimes were positively correlated with EA, EN, and PN (p < 0.05), the sensory experience was positively correlated with EN (p < 0.01), and diet did not correlate with childhood traumas. Stepwise regression analysis showed that dysfunctional attitude and emotional neglect were the main influencing factors of sensory experience (p < 0.001) and social interaction (p < 0.001). Dysfunctional attitude and physical neglect were the main factors influencing interest/pastimes (p < 0.001). SEM analysis found that dysfunctional mediated between childhood traumas and anhedonia.

Conclusions

The degree of anhedonia was related to dysfunctional attitudes and childhood traumas. The childhood emotional neglect experience was the most important and was related to sensory and social anhedonia. Dysfunctional attitudes played a mediating role between childhood neglect and anhedonia. Early psychotherapy targeting young adult MDD patients with childhood emotional neglect may help decrease symptoms of anhedonia.
The Impact of COVID-19 Epidemic on the Development of the Digital Economy of China—Based on the Data of 31 Provinces in China
Authors: Xu Aidi , Qian Fangbin , Pai Chih-Hung , Yu Na , Zhou Pan
Published: January 2022

This study investigates the impact of coronavirus disease 2019 (COVID-19) on economic development of China by measuring the HP financial index as an alternative variable of the digital economy. This study shows that economy of China developed further with the dissemination of COVID-19. Furthermore, the digital economy increased the level of economic development more prominently at the onset of COVID-19 pandemic. Moreover, an analysis of regional heterogeneity reveals that the eastern region maintained economic stability through its digital economy during COVID-19, while the central region improved its digital economy during COVID-19 pandemic. Although the economically underdeveloped western region has not suffered too seriously from COVID-19 pandemic, considering the sustained impact of disease and the uncertainty of its transmission speed, the region should vigorously develop its digital economy to manage public risk.
Online Rumor Diffusion Model Based on Variation and Silence Phenomenon in the Context of COVID-19
Authors: Ju Chunhua , Jiang Yihao , Bao Fuguang , Zou Bilin , Xu Chonghuan
Published: January 2022

In the era of mobile internet, information dissemination has made a new leap in speed and in breadth. With the outbreak of the coronavirus disease 2019 (COVID-19), the COVID-19 rumor diffusion that is not limited by time and by space often becomes extremely complex and fickle. It is also normal that a piece of unsubstantiated news about COVID-19 could develop to many versions. We focus on the stagnant role and information variants in the process of rumor diffusion about COVID-19, and through the study of variability and silence in the dissemination, which combines the effects of stagnation phenomenon and information variation on the whole communication system in the circulation of rumors about COVID-19, based on the classic rumor SIR (Susceptible Infected Recovered) model, we introduce a new concept of “variation” and “oyster”. The stability of the new model is analyzed by the mean field equation, and the threshold of COVID-19 rumor propagation is obtained later. According to the results of the simulation experiment, whether in the small world network or in the scale-free network, the increase of the immure and the silent probability of the variation can effectively reduce the speed of rumor diffusion about COVID-19 and is conducive to the dissemination of the truth in the whole population. Studies have also shown that increasing the silence rate of variation can reduce COVID-19 rumor transmission more quickly than the immunization rate. The interesting discovery is that at the same time, a higher rumor infection rate can bring more rumors about COVID-19 but does not always maintain a high number of the variation which could reduce variant tendency of rumors. The more information diffuses in the social group, the more consistent the version and content of the information will be, which proves that the more adequate each individual information is, the slower and less likely rumors about COVID-19 spread. This consequence tells us that the government needs to guide the public to the truth. Announcing the true information publicly could instantly contain the COVID-19 rumor diffusion well rather than making them hidden or voiceless.
Management of and Revitalization Strategy for Megacities Under Major Public Health Emergencies: A Case Study of Wuhan
Authors: Wu Xianguo , Chen Bin , Chen Hongyu , Feng Zongbao , Zhang Yun , Liu Yang
Published: January 2022

The outbreak of the COVID-19 pandemic in late 2019 has meant an uphill battle for city management. However, due to deficiencies in facilities and management experience, many megacities are less resilient when faced with such major public health events. Therefore, we chose Wuhan for a case study to examine five essential modules of urban management relevant to addressing the pandemic: (1) the medical and health system, (2) lifeline engineering and infrastructure, (3) community and urban management, (4) urban ecology and (5) economic development. The experience and deficiencies of each module in fighting the pandemic are analyzed, and strategies for revitalization and sustainable development in the future are proposed. The results show that in response to large-scale public health events, a comprehensive and coordinated medical system and good urban ecology can prevent the rapid spread of the epidemic. Additionally, good infrastructure and community management can maintain the operation of the city under the pandemic, and appropriate support policies are conducive to the recovery and development of the urban economy. These precedents provide insights and can serve as a reference for how to change the course of the pandemic in megacities that are still at risk, and they provide experience for responding to other pandemics.
Suicidal Ideation Is Associated With Excessive Smartphone Use Among Chinese College Students
Authors: Huang Qiuping , Lin Shuhong , Li Ying , Huang Shucai , Liao Zhenjiang , Chen Xinxin , Shao Tianli , Li Yifan , Cai Yi , Qi Jing , Shen Hongxian
Published: January 2022

Background

Suicidal ideation is the first step and a strong predictor of suicide. College students are at a considerably high risk of suicidal ideation, and smartphones are commonly used in this group. However, the relationship between suicidal ideation and smartphone use among Chinese college students is unclear. The current study aimed to investigate the prevalence of suicidal ideation among Chinese college students and its association with smartphone use and addiction factors.

Methods

A total of 439 college students participated the survey. We collected the demographic information, physical health, psychosocial factors (depressive symptoms, social support, sleep quality), characteristics of smartphone use, and mobile phone addiction (MPA). Suicidal ideation was measured with a single question, “did you feel that life was not worth living in the past 1 year?”

Results

The prevalence of suicidal ideation (“Yes” response) in the past year among Chinese college students was 7.5%. In binary logistic regression analysis, suicidal ideation was significantly correlated with less subjective social support (OR: 2.49,p= 0.049), lower utilization of social support (OR: 13.28,p= 0.012), more depressive symptoms (OR:4.96,p= 0.005), and more than 5 h of daily smartphone use (OR: 2.60,p= 0.025).

Conclusion

Considering the widely use of smartphones in Chinese colleges and the correlation with suicidal ideation, excessive phone use among college students should be given more attention by administrators and health workers. It is necessary to obtain more information about the intention of smartphone use, make full use of smartphones for health education, and monitor excessive use of smartphones, while improving social support and coping mechanisms for depression, to identify suicidal ideation and prevent suicidal behavior among Chinese college students.
Case Report: Bosworth Fracture-Dislocation managed by Closed Reduction and Conservative Treatment
Authors: Ji Wan-Bo , Xu Yao-Feng , Lu Zhen
Published: January 2022

Bosworth fracture-dislocation is a rare type of ankle injury, which in the typical radiologic are overlap of distal tibia and fibula in the anteroposterior view, posterior subluxation of the talus and syndesmosis dissociation in the lateral view, while in the CT scan, the fibula was displaced behind the posterior edge of the fibular notch (incisura tibiae), locked between the distal tibia and the displaced posterior malleolus fragment. Treatment can be challenging owing to the ignorance or failure of the initial reduction, resulting in an irreducible tibiotalar joint and tibiofibular syndesmosis reduction. To treat this complicated injury, emergent open surgery is always recommended for the first stage reduction to prevent adverse events. Successful closed reduction and conservative treatment of Bosworth fracture-dislocation is rare. This unique case presents a 25-year-old male with a Bosworth fracture-dislocation cured with closed reduction and U-shaped plaster splint. The patient was fully weight bearing and had no pain, and there were no limitations in the range of motion of the ankle and subtalar joints at 30 months of follow-up.
Direct Carotid Artery Exposure for Acute Cerebral Infarction in Hybrid Angiography Suite: Indications and Limitations
Authors: Chen Ching-Chang , Chen Chun-Ting , Wu Yi-Ming , Hsieh Po-Chuan , Yeap Mun-Chun , Chang Chien-Hung , Lin Chuan-Min , Chen Shao-Wei
Published: January 2022

Objectives

For the endovascular intervention of acute ischemic stroke, a transcervical route is an alternative approach in patients with challenging anatomical variations. Percutaneous puncture is a common way, but it can cause many fatal complications. Direct carotid artery exposure is an alternative for reducing complications. We demonstrate a technique of direct carotid exposure in patients for whom transfemoral or transbrachial approaches were impossible. We present patient outcomes and discuss the indications and limitations of this procedure.

Methods

We retrospectively reviewed the cases of patients undergoing direct carotid exposure for acute ischemic stroke in a hybrid angiography suite and presented the details of the technique.

Results

Among 548 consecutive patients with acute large vessel strokes who were treated by emergency endovascular thrombectomy or stenting between January 2015 and September 2020 in our center, only 8 (1.46%) required a transcervical approach. Of them, 7 underwent direct carotid exposure with successful recanalization and good clinical outcomes.

Conclusions

Direct carotid exposure for endovascular stroke treatment is effective and advantageous in patients with challenging anatomical variations. Performing this procedure in carefully selected patients in the hybrid angiography suite can be beneficial in terms of open surgeries, saving time, and decreasing the risk of postoperative complications.
The Evidence for a Causal Link Between Disease and Damaging Behavior in Pigs
Authors: Boyle Laura A. , Edwards Sandra A. , Bolhuis J. Elizabeth , Pol Françoise , Šemrov Manja Zupan , Schütze Sabine , Nordgreen Janicke , Bozakova Nadya , Sossidou Evangelia N. , Valros Anna
Published: January 2022

Damaging behaviors (DB) such as tail and ear biting are prevalent in pig production and reduce welfare and performance. Anecdotal reports suggest that health challenges increase the risk of tail-biting. The prevalence of tail damage and health problems show high correlations across batches within and between farms. There are many common risk factors for tail-biting and health problems, notably respiratory, enteric and locomotory diseases. These include suboptimal thermal climate, hygiene, stocking density and feed quality. The prevalence of tail damage and health problems also show high correlations across batches within and between farms. However, limited evidence supports two likely causal mechanisms for a direct link between DB and health problems. The first is that generalized poor health (e.g., enzootic pneumonia) on farm poses an increased risk of pigs performing DB. Recent studies indicate a possible causal link between an experimental inflammation and an increase in DB, and suggest a link between cytokines and tail-biting. The negative effects of poor health on the ingestion and processing of nutrients means that immune-stimulated pigs may develop specific nutrient deficiencies, increasing DB. The second causal mechanism involves tail-biting causing poor health. Indirectly, pathogens enter the body via the tail lesion and once infected, systemic spread of infection may occur. This occurs mainly via the venous route targeting the lungs, and to a lesser extent via cerebrospinal fluid and the lymphatic system. In carcasses with tail lesions, there is an increase in lung lesions, abscessation, arthritis and osteomyelitis. There is also evidence for the direct spread of pathogens between biters and victims. In summary, the literature supports the association between poor health and DB, particularly tail-biting. However, there is insufficient evidence to confirm causality in either direction. Nevertheless, the limited evidence is compelling enough to suggest that improvements to management and housing to enhance pig health will reduce DB. In the same way, improvements to housing and management designed to address DB, are likely to result in benefits to pig health. While most of the available literature relates to tail-biting, we suggest that similar mechanisms are responsible for links between health and other DB.
Barriers to Utilizing Non-replacement Male Calves in the Australian Dairy Industry: A Qualitative Study
Authors: Vicic Veronika , Saliba Anthony J. , Campbell Michael A. , Quinn Jane C.
Published: January 2022

Male non-replacement calves in dairy systems represent an underutilized economic resource for dairy producers worldwide. Despite this, increasing the practice of rearing non-replacement male calves has significant barriers both in on-farm adoption and practice. Poor neonatal rearing practices, higher levels of morbidity and mortality, and disaggregated production pathways with multiple points of handling, have all been described as barriers to adoption of surplus calf production. To identify the critical decision-determining challenges associated with broader adoption of raising non-replacement stock, and to investigate the whole-of-value chain issues faced by dairy producers to rear non-replacement male calves, we undertook a series of semi-structured interviews with Australian dairy producers to interrogate their key challenges. To achieve this, a constructivist grounded theory approach was used to inform the process of analysis of in-depth interviews with Australian dairy producers regarding their current practices and perceptions. Five major themes emerged from these conversations that were key barriers to on-farm non-replacement calf rearing in the producer group participants. These were: impacts of drought on cost and availability of feed for these calves and the whole herd; the management requirements of non-replacement male calves as an additional workload to that of their current operation; their attitudes and current practices to and surrounding euthanasia; perceived ease of supply-chain access for these calves, and their perceptions of the economic value of dairy-beef product as a return on investment. Understanding the barriers to adoption of non-replacement calf rearing, and addressing the value proposition for dairy beef, can assist increased uptake of non-replacement calf rearing. These findings will allow development of strategies to address these barriers, and extension of viable management strategies to increase adoption of profitable business practices surrounding non-replacement male calf production.
Effects of Confounding Factors on Liver Stiffness in Two-Dimensional Shear Wave Elastography in Beagle Dogs
Authors: Cha Jinwoo , Kim Jayon , Ko Jaeeun , Kim Jaehwan , Eom Kidong
Published: January 2022

Background

Two-dimensional shear wave elastography (2D-SWE) is a powerful technique that can non-invasively measure liver stiffness to assess hepatic fibrosis.

Purpose

This study aimed to identify the effects of confounding factors, including anesthesia, breathing, and scanning approach, on liver stiffness when performing 2D-SWE in dogs.

Materials and Methods

Nine healthy Beagle dogs were included in this study. Hepatic 2D-SWE was performed, and liver stiffness was compared between conscious and anesthetized states, free-breathing and breath-holding conditions, and intercostal and subcostal approaches. For the anesthetized state, the breath-holding condition was subdivided into seven phases, which included forced-expiration (5 and 10 mL/kg), end-expiration (0 cm H2O), and forced-inspiration (5, 10, 15, and 20 cm H2O), and liver stiffness was compared among these phases. Changes in liver stiffness were compared between intercostal and subcostal approaches according to breathing phases.

Results

No significant difference was observed in liver stiffness between the conscious and anesthetized states or between the free-breathing and breath-holding conditions. No significant difference was noted in liver stiffness among the breathing phases, except for forced-inspiration with high airway pressure (15 and 20 cm H2O in the intercostal approach and 10, 15, and 20 cm H2O in the subcostal approach), which was associated with significantly higher liver stiffness (p < 0.05). Liver stiffness was significantly higher in the subcostal approach than in the intercostal approach (p < 0.05). Changes in liver stiffness were significantly higher in the subcostal approach than in the intercostal approach in all forced-inspiratory phases (p < 0.05).

Conclusion

In conclusion, when performing 2D-SWE in dogs, liver stiffness is unaffected by anesthesia and free-breathing. To avoid inadvertent increases in liver stiffness, the deep inspiratory phase and subcostal approach are not recommended. Thus, liver stiffness should be interpreted considering these confounding factors.
Age at Time of Kidney Transplantation as a Predictor for Mortality, Graft Loss and Self-Rated Health Status: Results From the Swiss Transplant Cohort Study
Authors: Beerli Nadine , Denhaerynck Kris , Binet Isabelle , Dahdal Suzan , Dickenmann Michael , Golshayan Delaviz , Hadaya Karine , Huynh-Do Uyen , Schnyder Aurelia , De Geest Sabina M. , Mauthner Oliver
Published: January 2022

Introduction: The effect of age on health outcomes in kidney transplantation remains inconclusive. This study aimed to analyze the relationship between age at time of kidney transplantation with mortality, graft loss and self-rated health status in adult kidney transplant recipients.

Methods: This study used data from the Swiss Transplant Cohort Study and included prospective data of kidney transplant recipients between 2008 and 2017. Time-to-event analysis was performed using Cox’ regression analysis, and -in the case of graft loss- competing risk analysis. A random-intercept regression model was applied to analyse self-rated health status.

Results: We included 2,366 kidney transplant recipients. Age at transplantation linearly predicted mortality. It was also predictive for graft loss, though nonlinearly, showing that recipients aged between 35 and 55 years presented with the lowest risk of experiencing graft loss. No relationship of age with self-rated health status was detected.

Conclusion: Higher mortality in older recipients complies with data from the general population. The non-linear relationship between age and graft loss and the higher scored self-rated health status at all follow-up time-points compared to the pre-transplant status -regardless of age- highlight that age alone might not be an accurate measure for risk prediction and clinical decision making in kidney transplantation.
What Do These Findings Tell Us? Comment on Tinella et al. Cognitive Efficiency and Fitness-to-Drive along the Lifespan: The Mediation Effect of Visuospatial Transformations. Brain Sci. 2021, 11, 1028
Authors: Kelly Robert E. , Ahmed Anthony O. , Hoptman Matthew J.
Published: January 2022

Tinella et al.’s recent article [...]
Prenatal Detection of Congenital Duodenal Obstruction—Impact on Postnatal Care
Authors: Saalabian Kerstin , Friedmacher Florian , Theilen Till-Martin , Keese Daniel , Rolle Udo , Gfroerer Stefan
Published: January 2022

Background: Duodenal obstruction is a rare cause of congenital bowel obstruction. Prenatal ultrasound could be suggestive of duodenal atresia if polyhydramnios and the double bubble sign are visible. Prenatal diagnosis should prompt respective prenatal care, including surgery. The aim of this study was to investigate the rate and importance of prenatally diagnosed duodenal obstruction, comparing incomplete and complete duodenal obstruction. Methods: A retrospective, single-center study was performed using data from patients operated on for duodenal obstruction between 2004 and 2019. Prenatal ultrasound findings were obtained from maternal logbooks and directly from the investigating obstetricians. Postnatal data were obtained from electronic charts, including imaging, operative notes and follow-up. Results: A total of 33/64 parents of respective patients agreed to provide information on prenatal diagnostics. In total, 11/15 patients with complete duodenal obstruction and 0/18 patients with incomplete duodenal obstruction showed typical prenatal features. Prenatal diagnosis prompted immediate surgical treatment after birth. Conclusion: Prenatal diagnosis of congenital duodenal obstruction is only achievable in cases of complete congenital duodenal obstruction by sonographic detection of the pathognomonic double bubble sign. Patients with incomplete duodenal obstruction showed no sign of duodenal obstruction on prenatal scans and thus were diagnosed and treated later.
Length-Gauge Optical Matrix Elements in WIEN2k
Authors: Rubel Oleg , Blaha Peter
Published: January 2022

Hybrid exchange-correlation functionals provide superior electronic structure and optical properties of semiconductors or insulators as compared to semilocal exchange-correlation potentials due to admixing a portion of the non-local exact exchange potential from a Hartree–Fock theory. Since the non-local potential does not commute with the position operator, the momentum matrix elements do not fully capture the oscillator strength, while the length-gauge velocity matrix elements do. So far, length-gauge velocity matrix elements were not accessible in the all-electron full-potential WIEN2k package. We demonstrate the feasibility of computing length-gauge matrix elements in WIEN2k for a hybrid exchange-correlation functional based on a finite difference approach. To illustrate the implementation we determined matrix elements for optical transitions between the conduction and valence bands in GaAs, GaN, (CH3NH3)PbI3 and a monolayer MoS2. The non-locality of the Hartree–Fock exact exchange potential leads to a strong enhancement of the oscillator strength as noticed recently in calculations employing pseudopotentials (Laurien and Rubel: arXiv:2111.14772 (2021)). We obtained an analytical expression for the enhancement factor for the difference in eigenvalues not captured by the kinetic energy. It is expected that these results can also be extended to other non-local potentials, e.g., a many-body GW approximation.
Acknowledgment to Reviewers of Cosmetics in 2021
Authors:   Cosmetics Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Real-Time Improvement of a Trajectory-Tracking Control Based on Super-Twisting Algorithm for a Quadrotor Aircraft
Authors: González-Hernández Iván , Salazar Sergio , Lozano Rogelio , Ramírez-Ayala Oscar
Published: January 2022

This article addresses the development and experimental validation of a trajectory-tracking control for a miniature autonomous Quadrotor helicopter system (X4-prototype) using a robust algorithm control based on second-order sliding mode technique or also known as super-twisting algorithm in outdoor environments. This nonlinear control strategy guarantees the convergence in finite time to a desired path r(t) in the presence of external disturbances or uncertainties in the model affecting the appropriate behavior of our Quadrotor helicopter. For this purpose, a polynomial smooth curve trajectory is selected as a reference signal where the corresponding derivatives of the function are bounded. Moreover, we consider disturbances due to wind gusts acting on the aerial vehicle, and the reference signal is pre-programmed in an advanced autopilot system. The proposed solution consists of implementing a real-time control law based on super-twisting control using GPS measurements in order to obtain the position in the xy-plane to accomplish the desired trajectory. Simulation and experimental results of trajectory-tracking control are presented to demonstrate the performance and robustness of the proposed nonlinear controller in windy conditions.
Opinion Evolution in Divided Community
Authors: Weron Tomasz , Szwabiński Janusz
Published: January 2022

Our agent-based model of opinion dynamics concerns the current vast divisions in modern societies. It examines the process of social polarization, understood here as the partition of a community into two opposing groups with contradictory opinions. Our goal is to measure how mutual animosities between parties may lead to their radicalization. We apply a double-clique topology with both positive and negative ties to the model of binary opinions. Individuals are subject to social pressure; they conform to the opinions of their own clique (positive links) and oppose those from the other one (negative links). There is also a chance of acting independently, which alters the system’s behavior in various ways, depending on its magnitude. The results, obtained with both Monte-Carlo simulations and the mean-field approach, lead to two main conclusions: in such a system, there exists a critical quantity of negative relations that are needed for polarization to occur, and (rather surprisingly) independent actions actually support the process, unless their frequency is too high, in which case the system falls into total disorder.
Acknowledgment to Reviewers of Electricity in 2021
Authors:   Electricity Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Empirical Determination of Transitory and Persistent Delinquency in Chilean Youth: Validation of the Criminal Engagement Severity Scale “EGED”
Authors: Chesta Sergio , Pérez-Luco Ricardo , Alarcón Paula , Wenger Lorena , Concha-Salgado Andrés , García-Cueto Eduardo
Published: January 2022

Evidence from several longitudinal studies has established the relevance of the approach of evolutionary criminology to understanding and intervening with adolescent criminal offenders, seeking to halt the criminal behavior before its potential consolidation in adulthood. The aim of this study is to present the psychometric properties of the Criminal Engagement Severity Scale (EGED) to discriminate between transitory and persistent delinquency in Chilean adolescents of both sexes. The characteristics of the sample are revealed through descriptive analyses, and evidence of validity and reliability is provided that show its discriminant capacity using ROC curves and odds ratios, measures of internal consistency (Cronbach’s α and McDonald’s Ω), intraclass correlation, and unidimensional statistics. The results indicate that the EGED adequately discriminates between transitory and persistent delinquency, so that its use in contexts of assessment and intervention with adolescent criminal offenders can be recommended, because it helps to determine the intensity of the intervention required.
Deciphering the Genome-Wide Transcriptomic Changes during Interactions of Resistant and Susceptible Genotypes of American Elm with Ophiostoma novo-ulmi
Authors: Islam Md Tabibul , Coutin Jose Freixas , Shukla Mukund , Dhaliwal Amandeep Kaur , Nigg Martha , Bernier Louis , Sherif Sherif M. , Saxena Praveen K.
Published: January 2022

Dutch elm disease (DED), caused by Ophiostoma novo-ulmi (Onu), is a destructive disease of American elm (Ulmus americana L.). The molecular mechanisms of resistance and susceptibility against DED in American elm are still largely uncharacterized. In the present study, we performed a de novo transcriptome (RNA-sequencing; RNA-Seq) assembly of U. americana and compared the gene expression in a resistant genotype, ’Valley Forge’, and a susceptible (S) elm genotype at 0 and 96 h post-inoculation of Onu. A total of 85,863 non-redundant unigenes were identified. Compared to the previously characterized U. minor transcriptome, U. americana has 35,290 similar and 55,499 unique genes. The transcriptomic variations between ‘Valley Forge’ and ‘S’ were found primarily in the photosynthesis and primary metabolism, which were highly upregulated in the susceptible genotype irrespective of the Onu inoculation. The resistance to DED was associated with the activation of RPM1-mediated effector-triggered immunity that was demonstrated by the upregulation of genes involved in the phenylpropanoids biosynthesis and PR genes. The most significantly enriched gene ontology (GO) terms in response to Onu were response to stimulus (GO:0006950), response to stress (GO:0050896), and secondary metabolic process (GO:0008152) in both genotypes. However, only in the resistant genotype, the defense response (GO:0006952) was among the topmost significantly enriched GO terms. Our findings revealed the molecular regulations of DED resistance and susceptibility and provide a platform for marker-assisted breeding of resistant American elm genotypes.
3-Carbamoylmethyl-Indole-1-Carboxylic Acid Ethyl Ester
Authors: Mollova Yordanka , Angelov Plamen , Yanev Pavel
Published: January 2022

3-Carbamoylmethyl-Indole-1-Carboxylic Acid Ethyl Ester (an ethoxycarbonyl derivative of indole-3-acetamide) is obtained by Friedel–Crafts type cyclocondensation of γ-functionalized acetoacetamide in neat polyphosphoric acid.
Evaluation of the Antimicrobial Effect of Graphene Oxide Fiber on Fish Bacteria for Application in Aquaculture Systems
Authors: Lee Ji Hyun , Yoo Heejoun , Ahn Yu Jin , Kim Hyoung Jun , Kwon Se Ryun
Published: January 2022

The growing importance of the domestic aquaculture industry has led not only to its continuous development and expansion but also to an increase in the production of wastewater containing pathogenic microorganisms and antibiotic-resistant bacteria. As the existing water purification facilities have a high initial cost of construction, operation, and maintenance, it is necessary to develop an economical solution. Graphene oxide (GO) is a carbon-based nanomaterial that is easy to manufacture, inexpensive and has excellent antimicrobial properties. In this study, the antimicrobial effect of GO polyester fibers on seven species of fish pathogenic bacteria was analyzed to evaluate their effectiveness in water treatment systems and related products. As a result of incubating GO polyester fibers with seven types of fish pathogenic bacteria for 1, 6, and 12 h, there was no antimicrobial effect in Vibrio harveyi, V. scopthalmi, and Edwardsiella tarda. In contrast, GO fibers showed antimicrobial effects of more than 99% against A. hydrophila, S. parauberis, S. iniae, and P. piscicola, suggesting the potential use of GO fibers in water treatment systems.
Mono- and Multi-Objective CFD Optimization of Graded Foam-Filled Channels
Authors: Mauro Gerardo Maria , Iasiello Marcello , Bianco Nicola , Chiu Wilson K. S. , Naso Vincenzo
Published: January 2022

Graded foam-filled channels are a very promising solution for improving the thermal performance of heat sinks because of their customized structures that leave large amounts of room for heat transfer enhancement. Accordingly, this paper proposes a comprehensive optimization framework to address the design of such components, which are subjected to a uniform heat flux boundary condition. The graded foam is achieved by parameterizing the spatial distributions of porosity and/or Pores Per Inch (PPI). Mono- and multi-objective optimizations are implemented to find the best combination of the foam’s fluid-dynamic, geometrical and morphological design variables. The mono-objective approach addresses the Performance Evaluation Criterion (PEC) as an objective function to maximize the thermal efficiency of graded foams. The multi-objective approach addresses different objective functions by means of Pareto optimization to identify the optimal tradeoff solutions between heat transfer enhancement and pressure drop reduction. Optimizations are performed by assuming a local thermal non-equilibrium in the foam. They allowed us to achieve a 1.51 PEC value with H* = 0.50, ReH = 15000, iε = iPPI = 0.50, ε(0) = 0.85, ε(1) = 0.97, PPI(0) = 5, PPI(1) = 40, and ks→f = 104 as the design variables. For the three multi-objective functions investigated, one can extrapolate the optimum from the Pareto front via the utopia criterion, obtaining h¯ = 502 W/m2 K and Δp = 80 Pa, NuH,unif¯ = 2790 and f = 42, ⟨Ts*⟩s¯= 0.011, and Δp* = 91. The optimal solutions provide original insights and guidelines for the thermal design of graded foam-filled channels.
Effect of Welding Speed on Microstructure Evolution and Mechanical Properties of Friction Stir Welded 2198 Al-Cu-Li Alloy Joints
Authors: Zhang Wenyan , Mao Yuqing , Yang Ping , Li Ning , Ke Liming , Chen Yu
Published: January 2022

In the present study, 2198 Al-Cu-Li alloys were successfully friction stir welded by using various welding speed ranges of 90~180 mm/min with an invariable rotation speed of 950 r/min. The effect of welding speed on microstructure evolution and mechanical properties of the joints was investigated. The results show that, with the welding speed decreasing, the size of the nugget zone (NZ) first increases and then decreases due to different welding temperatures. At a welding speed of 150 mm/min, the size of the NZ in all joints is the biggest and the “S” curve disappears. The equiaxed grains are finer, attributed to a higher degree of dynamic recrystallization, and a larger number of fine reprecipitated phase (δ’, β’ phases) particles are dispersively distributed in the NZ. Correspondingly, the joints have the highest tensile properties, and the tensile strength, yield strength and elongation are, respectively, 406 MPa, 289 MPa and 7.2%. However, compared to the base material, the tensile properties of all joints are reduced because a greater amount of δ’ and β’ phases particles are dissolved in the NZ. Only the joints produced at 150 mm/min are fractured in the TMAZ with detected deep dimples and tearing ridges, and a significant necking phenomenon is observed, which indicates a complete ductile fracture mode.
The Effect of Grain Size on the Diffusion Bonding Properties of SP700 Alloy
Authors: Zhang Qianwen , Wu Jianjun , Jiang Shaosong , He Gang
Published: January 2022

Superplastic forming and diffusion bonding (SPF/DB) has been recognized as a viable manufacturing technology. However, the basic understanding of grain size and its effects on the quality of diffusion bonds is still limited. In this study, a certain type of SP700 alloy with different grain sizes is bonded at superplastic temperature. The experimental results indicate that the same materials, if coarse-grained, may not readily bond under identical conditions of pressure, temperature, and time. This type of bonding is possible because of the presence of many grain boundaries in fine-grained materials that act as short-circuit paths for diffusion. In addition, grain-boundary migration is also faster in fine-grained than in coarse-grained materials. Fractographic studies show that the dimples on the coarse-grained specimen have large dimensions compared with that in the fine-grained material, indicating that heterogeneous deformation develops in the coarse-grained specimen during tension.
Design of H-Shape Chamber in Thermal Bubble Printer
Authors: Peng Xishun , Lu Anjiang , Sun Qiliang , Xu Naitao , Xie Yibo , Wu Jiawen , Cheng Jin
Published: January 2022

The utilization rate of ink liquid in the chamber is critical for the thermal bubble inkjet head. The difficult problem faced by the thermal bubble inkjet printing is how to maximize the use of ink in the chamber and increase the printing frequency. In this paper, by adding a flow restrictor and two narrow channels into the chamber, the H-shape flow-limiting structure is formed. At 1.8 μs, the speed of bubble expansion reaches the maximum, and after passing through the narrow channel, the maximum reverse flow rate of ink decreased by 25%. When the vapor bubble disappeared, the ink fills the nozzle slowly. At 20 μs, after passing through the narrow channel, the maximum flow rate of the ink increases by 39%. The inkjet printing frequency is 40 kHz, and the volume of the ink droplet is about 13.1 pL. The structure improves the frequency of thermal bubble inkjet printing and can maximize the use of liquid in the chamber, providing a reference for cell printing, 3D printing, bioprinting, and other fields.
Genetic Diversity of Trypanosoma cruzi in Panama Inferred by Multi-locus Sequence Typing of Mitochondrial Genes
Authors: Calzada Jose E. , Samudio Franklyn , de Juncá Corina , Pineda Vanessa , Burleigh Barbara A. , Saldaña Azael
Published: January 2022

The objective of this study was to provide information on Trypanosoma cruzi genetic diversity among isolates obtained from different biological sources circulating in endemic areas of Panama. Initial discrete typing units (DTUs) assignment was performed evaluating three single locus molecular markers (mini-exon, heat shock protein 60 and glucose-6-phosphate isomerase genes). Further diversity within TcI lineages was explored using a multi-locus sequence typing approach with six maxicircle genes. Haplotype network analysis and evolutionary divergency estimations were conducted to investigate the genetic relatedness between Panamanian TcI isolates and isolates from different endemic regions in the Americas. Our molecular approach validated that TcI is the predominant DTU circulating in Panama across different hosts and vector species, but also confirmed the presence of TcIII and TcVI circulating in the country. The phylogenetic tree topography for most Panamanian TcI isolates displayed a high level of genetic homogeneity between them. The haplotype network analysis inferred a higher genetic diversity within Panamanian TcI isolates, displaying eight different haplotypes circulating in endemic regions of the country, and revealed geographical structuring among TcI from different endemic regions in the Americas. This study adds novelty on the genetic diversity of T. cruzi circulating in Panama and complements regional phylogeographic studies regarding intra-TcI variations.
Bacillus subtilis PS-216 Antagonistic Activities against Campylobacter jejuni NCTC 11168 Are Modulated by Temperature, Oxygen, and Growth Medium
Authors: Šimunović Katarina , Stefanic Polonca , Klančnik Anja , Erega Andi , Mandic Mulec Ines , Smole Možina Sonja Smole
Published: January 2022

As the incidence of Campylobacter jejuni and campylobacteriosis grows, so does the need for a better understanding and control of this pathogen. We studied the interactions of C. jejuni NCTC 11168 and a potential probiotic, Bacillus subtilis PS-216, in cocultures at different starting ratios and temperatures (20 °C, 37 °C, 42 °C), under different atmospheres (aerobic, microaerobic), and in different growth media (Mueller–Hinton, chicken litter medium, chicken intestinal-content medium). Under microaerobic conditions, B. subtilis effectively inhibited the growth of C. jejuni at 42 °C (log reduction, 4.19), even when C. jejuni counts surpassed B. subtilis by 1000-fold in the starting inoculum. This inhibition was weaker at 37 °C (log reduction, 1.63), while no impact on CFUs was noted at 20 °C, which is a temperature nonpermissive of C. jejuni growth. Under aerobic conditions, B. subtilis supported C. jejuni survival. B. subtilis PS-216 inhibited the growth of C. jejuni in sterile chicken litter (4.07 log reduction) and in sterile intestinal content (2.26 log reduction). In nonsterile intestinal content, B. subtilis PS-216 was able to grow, to a lesser extent, compared to Mueller–Hinton media, still showing potential as a chicken probiotic that could be integrated into the chicken intestinal microbiota. This study showed the strong influence of environmental parameters on the variability of C. jejuni and B. subtilis interactions. Furthermore, B. subtilis PS-216 antagonism was strongest against C. jejuni NCTC 11168 under conditions that might represent conditions in the chicken environment (42 °C, microaerobic atmosphere, chicken litter medium).
Dynamics of the Queensland Fruit Fly Microbiome through the Transition from Nature to an Established Laboratory Colony
Authors: Majumder Rajib , Taylor Phillip W. , Chapman Toni A.
Published: January 2022

The transition from nature to laboratory or mass rearing can impose significant physiological and evolutionary impact on insects. The Queensland fruit fly (also known as ‘Qfly’), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is a serious economic pest that presents major challenges for horticulture industries in Australia. The sterile insect technique (SIT) is being developed to manage outbreaks in regions that remain free of Qfly and to suppress populations in regions where this species is endemic. The biology of Qfly is intimately connected to its microbiome. Therefore, changes in the microbiome that occur through domestication have implications for SIT. There are numerous studies of the microbiome in Qfly larvae and adults, but there is little information on how the microbiome changes as Qfly laboratory colonies are established. In this study, high-throughput Illumina sequencing was used to assess the Qfly microbiome in colonies reared from wild larvae, collected from fruit, for five generations, on a gel-based larval diet. Beta diversity analysis showed that the bacterial communities from Generation 5 (G5) clustered separately from earlier generations. At the genus level, bacterial communities were significantly different between the generations and mostly altered at G5. However, communities were found similar at phyla to family taxonomic levels. We observed high abundance of Morganella and Burkholderia at the genus level in the larval and pupal stages respectively at G5, but these were not detected in earlier generations. Overall, our findings demonstrate that the domestication process strongly affects the Qfly microbiome and prompts questions about the functional relationship between the Qfly and its microbiome, as well as implications for the performance of insects that have been domesticated and mass-reared for SIT programs.
PREGO: A Literature and Data-Mining Resource to Associate Microorganisms, Biological Processes, and Environment Types
Authors: Zafeiropoulos Haris , Paragkamian Savvas , Ninidakis Stelios , Pavlopoulos Georgios A. , Jensen Lars Juhl , Pafilis Evangelos
Published: January 2022

To elucidate ecosystem functioning, it is fundamental to recognize what processes occur in which environments (where) and which microorganisms carry them out (who). Here, we present PREGO, a one-stop-shop knowledge base providing such associations. PREGO combines text mining and data integration techniques to mine such what-where-who associations from data and metadata scattered in the scientific literature and in public omics repositories. Microorganisms, biological processes, and environment types are identified and mapped to ontology terms from established community resources. Analyses of comentions in text and co-occurrences in metagenomics data/metadata are performed to extract associations and a level of confidence is assigned to each of them thanks to a scoring scheme. The PREGO knowledge base contains associations for 364,508 microbial taxa, 1090 environmental types, 15,091 biological processes, and 7971 molecular functions with a total of almost 58 million associations. These associations are available through a web portal, an Application Programming Interface (API), and bulk download. By exploring environments and/or processes associated with each other or with microbes, PREGO aims to assist researchers in design and interpretation of experiments and their results. To demonstrate PREGO’s capabilities, a thorough presentation of its web interface is given along with a meta-analysis of experimental results from a lagoon-sediment study of sulfur-cycle related microbes.
Trypanosomes of the Trypanosoma theileri Group: Phylogeny and New Potential Vectors
Authors: Brotánková Anna , Fialová Magdaléna , Čepička Ivan , Brzoňová Jana , Svobodová Milena
Published: January 2022

Trypanosomes belonging to Trypanosoma theileri group are mammalian blood parasites with keds and horse fly vectors. Our aim is to study to vector specificity of T. theileri trypanosomes. During our bloodsucking Diptera survey, we found a surprisingly high prevalence of T. theileri trypanosomes in mosquitoes (154/4051). Using PCR and gut dissections, we detected trypanosomes of T. theileri group mainly in Aedes mosquitoes, with the highest prevalence in Ae. excrucians (22%), Ae. punctor (21%), and Ae. cantans/annulipes (10%). Moreover, T. theileri group were found in keds and blackflies, which were reported as potential vectors for the first time. The vectorial capacity was confirmed by experimental infections of Ae. aegypti using our isolates from mosquitoes; sand fly Phlebotomus perniciosus supported the development of trypanosomes as well. Infection rates were high in both vectors (47–91% in mosquitoes, 65% in sandflies). Furthermore, metacyclic stages of T. theileri trypanosomes were observed in the gut of infected vectors; these putative infectious forms were found in the urine of Ae. aegypti after a second bloodmeal. On the contrary, Culex pipiens quinquefasciatus was refractory to experimental infections. According to a phylogenetic analysis of the 18S rRNA gene, our trypanosomes belong into three lineages, TthI, ThII, and a lineage referred to as here a putative lineage TthIII. The TthI lineage is transmitted by Brachycera, while TthII and ThIII include trypanosomes from Nematocera. In conclusion, we show that T. theileri trypanosomes have a wide range of potential dipteran vectors, and mosquitoes and, possibly, sandflies serve as important vectors.