bluuebunny/crossref_metadata_2025 huggingface dataset:
Hot Corrosion Behavior of Inconel 625 in Na2SO4 and V2O5 Molten Salt System
Authors: Li Liang , Li Lanfeng , Zhang Guofeng , Xue Hongdi , Cui Maomao , Wang Wenxu , Liu Dexue
Published: June 2023

This study aimed to examine the corrosion behavior of Inconel 625 in a molten salt system of sodium sulfate and vanadium pentoxide at varying temperatures and durations. The corrosion products, microstructure, and element distribution of hot extruded Inconel in Na2SO4 and V2O5 molten salt systems were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) analyses. This study demonstrates that corrosion of the alloy increases with time at a constant temperature. During the initial stage of corrosion, the surface of the alloy is primarily composed of a dense oxide layer consisting of Cr2O3 and NiO. However, after exposure to the salt bath for 24 h, a chemical reaction occurs between the alloy and vanadium (V), resulting in the formation of CrVO4 and Ni3V2O8. Furthermore, the intrusion of sulfur (S) element into the matrix leads to the formation of internal sulfides, including Ni-, Cr-, and Mo-based sulfides, which accelerate intergranular and intracrystalline corrosion. As the corrosion temperature rises, the surface microstructure of the corrosion layer transforms from powder to salt particles and then to massive particles. The corrosion products exhibit a clear stratification, while the alloy undergoes simultaneous oxidation and vulcanization processes.
Rapid Calibration of Nanoliter per Second Flow Rate by Image Processing Technology
Authors: Luo Jiawei , Yang Cheng , Shen Yan
Published: June 2023

The need for high-precision microflow control is increasingly evident across various fields. For instance, microsatellites employed in gravitational wave detection require flow supply systems with a high accuracy of up to 0.1 nL/s to achieve on-orbit attitude control and orbit control. However, conventional flow sensors are unable to provide the necessary precision in the nanoliter per second range, and thus, alternative methods are required. In this study, we propose the use of image processing technology for rapid microflow calibration. Our method involves capturing images of the droplets at the outlet of the flow supply system to rapidly obtain the flow rate, and we used the gravimetric method to verify the accuracy of our approach. We conducted several microflow calibration experiments within the 1.5 nL/s range and demonstrated that image processing technology can achieve the desired accuracy of 0.1 nL/s while saving more than two-thirds of the time required to obtain the flow rate within an acceptable margin of error compared to the gravimetric method. Our study presents an efficient and innovative approach to addressing the challenges of measuring microflows with high precision, particularly in the nanoliter per second range, and has the potential for widespread applications in various fields.
Exploring the Cholesterol-Modifying Abilities of Lactobacilli Cells in Digestive Models and Dairy Products
Authors: Ziarno Małgorzata , Zaręba Dorota , Ścibisz Iwona , Kozłowska Mariola
Published: June 2023

This study aimed to investigate the ability of lactic acid bacteria to remove cholesterol in simulated gastric and intestinal fluids. The findings showed that the amount of cholesterol removed was dependent on the biomass, viability, and bacterial strain. Some cholesterol binding was stable and not released during gastrointestinal transit. The presence of cholesterol affected the fatty acid profile of bacterial cells, potentially influencing their metabolism and functioning. However, adding cholesterol did not significantly impact the survival of lactic acid bacteria during gastrointestinal transit. Storage time, passage, and bacterial culture type did not show significant effects on cholesterol content in fermented dairy products. Variations in cell survival were observed among lactic acid bacteria strains in simulated gastric and intestinal fluids, depending on the environment. Higher milk protein content was found to be more protective for bacterial cells during gastrointestinal transit than fat content. Future research should aim to better understand the impact of cholesterol on lactic acid bacteria metabolism and identify potential health benefits.
Nyrdvomenshor Nephrite Deposit, Polar Urals, Russia
Authors: Kislov Evgeniy V. , Popov Mikhail P. , Nurmukhametov Firat M. , Posokhov Viktor F. , Vanteev Vladislav V.
Published: June 2023

We studied the quality characteristics, chemical, mineral and isotope composition of nephrite, diopsidite and rodingite of the Nyrdvomenshor nephrite deposit in the Polar Urals. We applied visual petrographic and mineralogical studies, X-ray spectral fluorescence, ICP-MS analysis, and a scanning electron microscope with a dispersive microanalysis system, to measure the oxygen isotope composition. According to its quality characteristics, the nephrite was substandard. Here, uvarovite, which forms idiomorphic grains, sometimes sheath-like and less often xenomorphic elongated, and substituting the chromite, was commonly encountered. The nephrite was formed due to both metamorphic and metasomatic processes. The serpentinite was replaced by diopsidite, which was then replaced by nephrite. The metamorphism intensified the metasomatism of the serpentinite melange and provided the cryptocrystalline tangled-fibrous structure of the nephrite. Then, metamorphism and metasomatism led to the formation of omphacite and the cracking of the nephrite, which reduced its quality. As these processes progressed, the contribution of the crustal fluid increased.
Plasmonic Cu2−xSe Mediated Colorimetric/Photothermal Dual-Readout Detection of Glutathione
Authors: Yan Guojuan , Ni Huanhuan , Li Xiaoxiao , Qi Xiaolan , Yang Xi , Zou Hongyan
Published: June 2023

Plasmonic nanomaterials have attracted great attention in the field of catalysis and sensing for their outstanding electrical and optical properties. Here, a representative type of nonstoichiometric Cu2−xSe nanoparticles with typical near-infrared (NIR) localized surface plasma resonance (LSPR) properties originating from their copper deficiency was applied to catalyze the oxidation of colorless TMB into their blue product in the presence of H2O2, indicating they had good peroxidase-like activity. However, glutathione (GSH) inhibited the catalytic oxidation of TMB, as it can consume the reactive oxygen species. Meanwhile, it can induce the reduction of Cu(II) in Cu2−xSe, resulting in a decrease in the degree of copper deficiency, which can lead to a reduction in the LSPR. Therefore, the catalytic ability and photothermal responses of Cu2−xSe were decreased. Thus, in our work, a colorimetric/photothermal dual-readout array was developed for the detection of GSH. The linear calibration for GSH concentration was in the range of 1–50 μM with the LOD as 0.13 μM and 50–800 μM with the LOD as 39.27 μM. To evaluate the practicability of the assay, tomatoes and cucumbers were selected as real samples, and good recoveries indicated that the developed assay had great potential in real applications.
Bioinspired Hierarchical Carbon Structures as Potential Scaffolds for Wound Healing and Tissue Regeneration Applications
Authors: Parikh Soham D. , Wang Wenhu , Nelson M. Tyler , Sulentic Courtney E. W. , Mukhopadhyay Sharmila M.
Published: June 2023

Engineered bio-scaffolds for wound healing provide an attractive treatment option for tissue engineering and traumatic skin injuries since they can reduce dependence on donors and promote faster repair through strategic surface engineering. Current scaffolds present limitations in handling, preparation, shelf life, and sterilization options. In this study, bio-inspired hierarchical all-carbon structures comprising carbon nanotube (CNT) carpets covalently bonded to flexible carbon fabric have been investigated as a platform for cell growth and future tissue regeneration applications. CNTs are known to provide guidance for cell growth, but loose CNTs are susceptible to intracellular uptake and are suspected to cause in vitro and in vivo cytotoxicity. This risk is suppressed in these materials due to the covalent attachment of CNTs on a larger fabric, and the synergistic benefits of nanoscale and micro-macro scale architectures, as seen in natural biological materials, can be obtained. The structural durability, biocompatibility, tunable surface architecture, and ultra-high specific surface area of these materials make them attractive candidates for wound healing. In this study, investigations of cytotoxicity, skin cell proliferation, and cell migration were performed, and results indicate promise in both biocompatibility and directed cell growth. Moreover, these scaffolds provided cytoprotection against environmental stressors such as Ultraviolet B (UVB) rays. It was seen that cell growth could also be tailored through the control of CNT carpet height and surface wettability. These results support future promise in the design of hierarchical carbon scaffolds for strategic wound healing and tissue regeneration applications.
Preparation of Hybrid Nanopigments with Excellent Environmental Stability, Antibacterial and Antioxidant Properties Based on Monascus Red and Sepiolite by One-Step Grinding Process
Authors: Li Shue , Yan Penji , Mu Bin , Kang Yuru , Wang Aiqin
Published: June 2023

This study is focused on the preparation, characterization, and multifunctional properties of intelligent hybrid nanopigments. The hybrid nanopigments with excellent environmental stability and antibacterial and antioxidant properties were fabricated based on natural Monascus red, surfactant, and sepiolite via a facile one-step grinding process. The density functional theory calculations demonstrated that the surfactants loaded on sepiolite were in favor of enhancing the electrostatic, coordination, and hydrogen bonding interactions between Monascus red and sepiolite. Thus, the obtained hybrid nanopigments exhibited excellent antibacterial and antioxidant properties, with an inhibition effect on Gram-positive bacteria that was superior to that of Gram-negative bacteria. In addition, the scavenging activity on DPPH and hydroxyl free radicals as well as the reducing power of hybrid nanopigments were higher than those of hybrid nanopigments prepared without the addition of the surfactant. Inspired by nature, gas-sensitive reversible alochroic superamphiphobic coatings with excellent thermal and chemical stability were successfully designed by combining hybrid nanopigments and fluorinated polysiloxane. Therefore, intelligent multifunctional hybrid nanopigments have great application foreground in related fields.
Clinical Pathobiochemistry of Vitamin B12 Deficiency: Improving Our Understanding by Exploring Novel Mechanisms with a Focus on Diabetic Neuropathy
Authors: Schleicher Erwin , Didangelos Triantafyllos , Kotzakioulafi Evangelia , Cegan Alexander , Peter Andreas , Kantartzis Konstantinos
Published: June 2023

Vitamin B12 (B12) is an essential cofactor of two important biochemical pathways, the degradation of methylmalonic acid and the synthesis of methionine from homocysteine. Methionine is an important donor of methyl groups for numerous biochemical reactions, including DNA synthesis and gene regulation. Besides hematological abnormalities (megaloblastic anemia or even pancytopenia), a deficiency in B12 may cause neurological symptoms, including symptoms resembling diabetic neuropathy. Although extensively studied, the underlining molecular mechanism for the development of diabetic peripheral neuropathy (DPN) is still unclear. Most studies have found a contribution of oxidative stress in the development of DPN. Detailed immunohistochemical investigations in sural nerve biopsies obtained from diabetic patients with DPN point to an activation of inflammatory pathways induced via elevated advanced glycation end products (AGE), ultimately resulting in increased oxidative stress. Similar results have been found in patients with B12 deficiency, indicating that the observed neural changes in patients with DPN might be caused by cellular B12 deficiency. Since novel results show that B12 exerts intrinsic antioxidative activity in vitro and in vivo, B12 may act as an intracellular, particularly as an intramitochondrial, antioxidant, independent from its classical, well-known cofactor function. These novel findings may provide a rationale for the use of B12 for the treatment of DPN, even in subclinical early states.
Soy-Based Tempeh Rich in Paraprobiotics Properties as Functional Sports Food: More Than a Protein Source
Authors: Subali Dionysius , Christos Revelo , Givianty Vasya , Ranti Alberta , Kartawidjajaputra Felicia , Antono Lina , Dijaya Rendy , Taslim Nurpudji , Rizzo Gianluca , Nurkolis Fahrul
Published: June 2023

To date, there has been no recent opinion that explores tempeh as a functional food that can improve sports performance. Hence, this opinion article aims to elaborate on recent findings on the potential effect on sports performance of soy-based tempeh. This opinion paper presents updated evidence based on literature reviews about soy-based tempeh and its relationship with sports performance. The paraprobiotic role of Lactobacillus gasseri for athletes has been found to restore fatigue and reduce anxiety. This is achieved by increasing protein synthesis activity in eukaryotic initiation factor-2 (EIF2) signaling known as an adaptive pathway for integrated stress response. In addition, these paraprobiotics prevent down-regulation associated with the oxidative phosphorylation gene, thereby contributing to the maintenance of mitochondrial function and recovery from fatigue. The authors believe that this opinion article will encourage researchers to continue to evolve soybean-based tempeh food products and increase the performance of athletes by consuming soy-based foods.
Alleviation Syndrome of High-Cholesterol-Diet-Induced Hypercholesterolemia in Mice by Intervention with Lactiplantibacillus plantarum WLPL21 via Regulation of Cholesterol Metabolism and Transportation as Well as Gut Microbiota
Authors: Zhao Kui , Qiu Liang , He Yao , Tao Xueying , Zhang Zhihong , Wei Hua
Published: June 2023

Probiotics are prospective for the prevention and treatment of cardiovascular diseases. Until now, systematic studies on the amelioration of hypercholesterolemia have been rare in terms of (cholesterol metabolism and transportation, reshaping of gut microbiota, as well as yielding SCFAs) intervention with lactic acid bacteria (LAB). In this study, strains of Lactiplantibacillus plantarum, WLPL21, WLPL72, and ZDY04, from fermented food and two combinations (Enterococcus faecium WEFA23 with L. plantarum WLPL21 and WLPL72) were compared for their effect on hypercholesterolemia. Comprehensively, with regard to the above aspects, L. plantarum WLPL21 showed the best mitigatory effect among all groups, which was revealed by decreasing total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels, upregulated cholesterol metabolism (Cyp27a1, Cyp7b1, Cyp7a1, and Cyp8b1) levels in the liver, cholesterol transportation (Abca1, Abcg5, and Abcg8) in the ileum or liver, and downregulated Npc1l1. Moreover, it reshaped the constitution of gut microbiota; specifically, the ratio of Firmicutes to Bacteroidetes (F/B) was downregulated; the relative abundance of Allobaculum, Blautia, and Lactobacillus was upregulated by 7.48–14.82-fold; and that of Lachnoclostridium and Desulfovibrio was then downregulated by 69.95% and 60.66%, respectively. In conclusion, L. plantarum WLPL21 improved cholesterol metabolism and transportation, as well as the abundance of gut microbiota, for alleviating high-cholesterol-diet-induced hypercholesterolemia.
Influence of Meal Sequence and Number of Teeth Present on Nutrient Intake Status: A Cross-Sectional Study
Authors: Kida Sayuri , Aoyama Norio , Fujii Toshiya , Taniguchi Kentaro , Yata Tomomi , Iwane Taizo , Yamamoto Tatsuo , Tamaki Katsushi , Minabe Masato , Komaki Motohiro
Published: June 2023

Intake of fiber, as well as protein, and lipid preloading help to control postprandial glycemic elevation in people with type 2 diabetes and in healthy individuals. However, there are few studies on the awareness of meal sequence and nutrient intake status that consider oral conditions. This cross-sectional study aimed to determine the effects of meal sequences on nutrient intake status and whether these relationships were related to the number of teeth present. The subjects were recruited from the Medical and Dental Collaboration Center of Kanagawa Dental University Hospital between 2018 and 2021. Medical and dental examinations were performed, and a questionnaire was used to determine whether the diet consisted of vegetables, meat or fish, and carbohydrates in that order. Nutrient intake status was assessed using the brief-type self-administered diet history questionnaire. Data were collected from 238 participants. The group with awareness of meal sequence ingested increased nutrients such as n-3 fatty acids, total dietary fiber, calcium, and vitamin C. Saturated fatty acid intake increased in those with fewer teeth, while it was not significantly related to meal sequence. In conclusion, our results showed that meal sequence was associated with nutrient intake status. In addition, the intake of saturated fatty acids increased when many teeth were lost, regardless of meal sequence.
Hematopoietic Stem Cell: Regulation and Nutritional Intervention
Authors: Sun Siyuan , Han Yingxue , Lei Yumei , Yu Yifei , Dong Yanbin , Chen Juan
Published: June 2023

Hematopoietic stem cells (HSCs) are crucial for the life maintenance of bio-organisms. However, the mechanism of HSC regulation is intricate. Studies have shown that there are various factors, either intrinsically or extrinsically, that shape the profile of HSCs. This review systematically summarizes the intrinsic factors (i.e., RNA-binding protein, modulators in epigenetics and enhancer–promotor-mediated transcription) that are reported to play a pivotal role in the function of HSCs, therapies for bone marrow transplantation, and the relationship between HSCs and autoimmune diseases. It also demonstrates the current studies on the effects of high-fat diets and nutrients (i.e., vitamins, amino acids, probiotics and prebiotics) on regulating HSCs, providing a deep insight into the future HSC research.
Biology, Control and Zoonotic Role of Disease Vectors
Authors: Morchón Rodrigo , Bueno-Marí Rubén , Bravo-Barriga Daniel
Published: June 2023

Vector-borne diseases result from the transmission of pathogens, including bacteria, parasites or viruses, by different hematophagous insects such as mosquitoes, phlebotomine sandflies, black flies, ticks, fleas, lice and triatomines, among others [...]
Application of Zebrafish as a Model for Anti-Cancer Activity Evaluation and Toxicity Testing of Natural Products
Authors: Shen Yifan , Sheng Ruilong , Guo Ruihua
Published: June 2023

Developing natural product-based anti-cancer drugs/agents is a promising way to overcome the serious side effects and toxicity of traditional chemotherapeutics for cancer treatment. However, rapid assessment of the in vivo anti-cancer activities of natural products is a challenge. Alternatively, zebrafish are useful model organisms and perform well in addressing this challenging issue. Nowadays, a growing number of studies have utilized zebrafish models to evaluate the in vivo activities of natural compounds. Herein, we reviewed the application of zebrafish models for evaluating the anti-cancer activity and toxicity of natural products over the past years, summarized its process and benefits, and provided future outlooks for the development of natural product-based anti-cancer drugs.
Tocotrienol-Rich Fraction Ameliorates the Aluminium Chloride-Induced Neurovascular Dysfunction-Associated Vascular Dementia in Rats
Authors: Shaikh Sohrab A. , Muthuraman Arunachalam
Published: June 2023

Neurovascular dysfunction leads to the second most common type of dementia, i.e., vascular dementia (VaD). Toxic metals, such as aluminium, increase the risk of neurovascular dysfunction-associated VaD. Hence, we hypothesized that a natural antioxidant derived from palm oil, i.e., tocotrienol-rich fraction (TRF), can attenuate the aluminium chloride (AlCl3)-induced VaD in rats. Rats were induced with AlCl3 (150 mg/kg) intraperitoneally for seven days followed by TRF treatment for twenty-one days. The elevated plus maze test was performed for memory assessment. Serum nitrite and plasma myeloperoxidase (MPO) levels were measured as biomarkers for endothelial dysfunction and small vessel disease determination. Thiobarbituric acid reactive substance (TBARS) was determined as brain oxidative stress marker. Platelet-derived growth factor-C (PDGF-C) expression in the hippocampus was identified using immunohistochemistry for detecting the neovascularisation process. AlCl3 showed a significant decrease in memory and serum nitrite levels, while MPO and TBARS levels were increased; moreover, PDGF-C was not expressed in the hippocampus. However, TRF treatment significantly improved memory, increased serum nitrite, decreased MPO and TBARS, and expressed PDGF-C in hippocampus. Thus, the results imply that TRF reduces brain oxidative stress, improves endothelial function, facilitates hippocampus PDGF-C expression for neovascularisation process, protects neurons, and improves memory in neurovascular dysfunction-associated VaD rats.
In Silico Development of Novel Benzofuran-1,3,4-Oxadiazoles as Lead Inhibitors of M. tuberculosis Polyketide Synthase 13
Authors: Irfan Ali , Faisal Shah , Zahoor Ameer Fawad , Noreen Razia , Al-Hussain Sami A. , Tuzun Burak , Javaid Rakshanda , Elhenawy Ahmed A. , Zaki Magdi E. A. , Ahmad Sajjad , Abdellattif Magda H.
Published: June 2023

Benzofuran and 1,3,4-oxadiazole are privileged and versatile heterocyclic pharmacophores which display a broad spectrum of biological and pharmacological therapeutic potential against a wide variety of diseases. This article reports in silico CADD (computer-aided drug design) and molecular hybridization approaches for the evaluation of the chemotherapeutic efficacy of 16 S-linked N-phenyl acetamide moiety containing benzofuran-1,3,4-oxadiazole scaffolds BF1–BF16. This virtual screening was carried out to discover and assess the chemotherapeutic efficacy of BF1–BF16 structural motifs as Mycobacterium tuberculosis polyketide synthase 13 (Mtb Pks13) enzyme inhibitors. The CADD study results revealed that the benzofuran clubbed oxadiazole derivatives BF3, BF4, and BF8 showed excellent and remarkably significant binding energies against the Mtb Pks13 enzyme comparable with the standard benzofuran-based TAM-16 inhibitor. The best binding affinity scores were displayed by 1,3,4-oxadiazoles-based benzofuran scaffolds BF3 (−14.23 kcal/mol), BF4 (−14.82 kcal/mol), and BF8 (−14.11 kcal/mol), in comparison to the binding affinity score of the standard reference TAM-16 drug (−14.61 kcal/mol). 2,5-Dimethoxy moiety-based bromobenzofuran-oxadiazole derivative BF4 demonstrated the highest binding affinity score amongst the screened compounds, and was higher than the reference Pks13 inhibitor TAM-16 drug. The bindings of these three leads BF3, BF4, and BF8 were further confirmed by the MM-PBSA investigations in which they also exhibited strong bindings with the Pks13 of Mtb. Moreover, the stability analysis of these benzofuran-1,3,4-oxadiazoles in the active sites of the Pks13 enzyme was achieved through molecular dynamic (MD) simulations at 250 ns virtual simulation time, which indicated that these three in silico predicted bio-potent benzofuran tethered oxadiazole molecules BF3, BF4, and BF8 demonstrated stability with the active site of the Pks13 enzyme.
Beneficial Effects of Zoledronic Acid on Tendons of the Osteogenesis Imperfecta Mouse (Oim)
Authors: Chretien Antoine , Mabilleau Guillaume , Lebacq Jean , Docquier Pierre-Louis , Behets Catherine
Published: June 2023

Osteogenesis imperfecta (OI) is a genetic disorder of connective tissue characterized by spontaneous fractures, bone deformities, impaired growth and posture, as well as extra-skeletal manifestations. Recent studies have underlined an impairment of the osteotendinous complex in mice models of OI. The first objective of the present work was to further investigate the properties of tendons in the osteogenesis imperfecta mouse (oim), a model characterized by a mutation in the COL1A2 gene. The second objective was to identify the possible beneficial effects of zoledronic acid on tendons. Oim received a single intravenous injection of zoledronic acid (ZA group) at 5 weeks and were euthanized at 14 weeks. Their tendons were compared with those of untreated oim (oim group) and control mice (WT group) by histology, mechanical tests, western blotting and Raman spectroscopy. The ulnar epiphysis had a significantly lower relative bone surface (BV/TV) in oim than WT mice. The tendon of the triceps brachii was also significantly less birefringent and displayed numerous chondrocytes aligned along the fibers. ZA mice showed an increase in BV/TV of the ulnar epiphysis and in tendon birefringence. The tendon of the flexor digitorum longus was significantly less viscous in oim than WT mice; in ZA-treated mice, there was an improvement of viscoelastic properties, especially in the toe region of stress-strain curve, which corresponds to collagen crimp. The tendons of both oim and ZA groups did not show any significant change in the expression of decorin or tenomodulin. Finally, Raman spectroscopy highlighted differences in material properties between ZA and WT tendons. There was also a significant increase in the rate of hydroxyproline in the tendons of ZA mice compared with oim ones. This study highlighted changes in matrix organization and an alteration of mechanical properties in oim tendons; zoledronic acid treatment had beneficial effects on these parameters. In the future, it will be interesting to better understand the underlying mechanisms which are possibly linked to a greater solicitation of the musculoskeletal system.
Biopolymer-Based Nanogel Approach in Drug Delivery: Basic Concept and Current Developments
Authors: Altuntaş Ebru , Özkan Burcu , Güngör Sevgi , Özsoy Yıldız
Published: June 2023

Due to their increased surface area, extent of swelling and active substance-loading capacity and flexibility, nanogels made from natural and synthetic polymers have gained significant interest in scientific and industrial areas. In particular, the customized design and implementation of nontoxic, biocompatible, and biodegradable micro/nano carriers makes their usage very feasible for a range of biomedical applications, including drug delivery, tissue engineering, and bioimaging. The design and application methodologies of nanogels are outlined in this review. Additionally, the most recent advancements in nanogel biomedical applications are discussed, with particular emphasis on applications for the delivery of drugs and biomolecules.
A Foundational Population Genetics Investigation of the Sexual Systems of Solanum (Solanaceae) in the Australian Monsoon Tropics Suggests Dioecious Taxa May Benefit from Increased Genetic Admixture via Obligate Outcrossing
Authors: Cantley Jason T. , Jordon-Thaden Ingrid E. , Roche Morgan D. , Hayes Daniel , Kate Stephanie , Martine Christopher T.
Published: June 2023

Solanum section Leptostemonum is an ideal lineage to test the theoretical framework regarding proposed evolutionary benefits of outcrossing sexual systems in comparison to cosexuality. Theoretically, non-cosexual taxa should support more genetic diversity within populations, experience less inbreeding, and have less genetic structure due to a restricted ability to self-fertilize. However, many confounding factors present challenges for a confident inference that inherent differences in sexual systems influence observed genetic patterns among populations. This study provides a foundational baseline of the population genetics of several species of different sexual systems with the aim of generating hypotheses of any factor—including sexual system—that influences genetic patterns. Importantly, results indicate that dioecious S. asymmetriphyllum maintains less genetic structure and greater admixture among populations than cosexual S. raphiotes at the same three locations where they co-occur. This suggests that when certain conditions are met, the evolution of dioecy may have proceeded as a means to avoid genetic consequences of self-compatibility and may support hypotheses of benefits gained through differential resource allocation partitioned across sexes. Arguably, the most significant finding of this study is that all taxa are strongly inbred, possibly reflective of a shared response to recent climate shifts, such as the increased frequency and intensity of the region’s fire regime.
Identification of Potential Phytochemical/Antimicrobial Agents against Pseudoperonospora cubensis Causing Downy Mildew in Cucumber through In-Silico Docking
Authors: Jhansirani Nagaraju , Devappa Venkatappa , Sangeetha Chittarada Gopal , Sridhara Shankarappa , Shankarappa Kodegandlu Subbanna , Mohanraj Mooventhiran
Published: June 2023

Compatibility interactions between the host and the fungal proteins are necessary to successfully establish a disease in plants by fungi or other diseases. Photochemical and antimicrobial substances are generally known to increase plant resilience, which is essential for eradicating fungus infections. Through homology modeling and in silico docking analysis, we assessed 50 phytochemicals from cucumber (Cucumis sativus), 15 antimicrobial compounds from botanical sources, and six compounds from chemical sources against two proteins of Pseudoperonospora cubensis linked to cucumber downy mildew. Alpha and beta sheets made up the 3D structures of the two protein models. According to Ramachandran plot analysis, the QNE 4 effector protein model was considered high quality because it had 86.8% of its residues in the preferred region. The results of the molecular docking analysis showed that the QNE4 and cytochrome oxidase subunit 1 proteins of P. cubensis showed good binding affinities with glucosyl flavones, terpenoids and flavonoids from phytochemicals, antimicrobial compounds from botanicals (garlic and clove), and chemically synthesized compounds, indicating the potential for antifungal activity.
Cytotoxic, Anti-Hemolytic, and Antioxidant Activities of Ruta chalepensis L. (Rutaceae) Extract, Fractions, and Isolated Compounds
Authors: Elizondo-Luévano Joel H. , Rodríguez-Garza Nancy E. , Bazaldúa-Rodríguez Aldo F. , Romo-Sáenz César I. , Tamez-Guerra Patricia , Verde-Star María J. , Gomez-Flores Ricardo , Quintanilla-Licea Ramiro
Published: June 2023

Ruta chalepensis is an herb used to treat various ailments, and its potential cytotoxic effects on different tumor cell lines have been extensively studied. The present study aimed to evaluate the cytotoxic activity of R. chalepensis methanol extract (RCME), sub-partitions obtained from solvents of increasing polarity, and major compounds, as well as their hemolytic, anti-hemolytic, and antioxidant potential. The in vitro cytotoxic activity against the human hepatocarcinoma (HEP-G2) and the murine lymphoma cell line (L5178Y-R) was evaluated using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, whereas selectivity indices (SIs) were determined by comparing cytotoxicity against normal African green monkey kidney cells (VERO) and human peripheral blood mononuclear cells (PBMC). Hemolytic and anti-hemolytic activities were evaluated on human erythrocytes. The most effective cytotoxic treatment was evaluated for nitric oxide release by J774A.1 macrophages. Antioxidant activity of R. chalepensis material was also determined. Results showed that RCME produced significant (p < 0.05) cytotoxicity in HEP-G2 (IC50 = 1.79 µg/mL) and L5178Y-R (IC50 = 1.60 µg/mL) cells and exhibited high SIs (291.50 and 114.80, respectively). In addition, the n-hexane fraction (RCHF) showed an IC50 of 18.31 µg/mL in HEP-G2 cells and an SI of 9.48 in VERO cells, whereas the chloroform fraction (RCCF) evidenced an IC50 of 1.60 µg/mL in L5178Y-R cells and an SI of 34.27 in PBMC cells. Chalepensin (CHL), rutamarin (RTM), and graveolin (GRV), which are major components of R. chalepensis, showed high activity against L5178Y-R cells, with IC50 of 9.15, 15.13 and SI of 45.08 µg/mL, respectively. In addition, CHL, RTM, and GRV showed SIs of 24.76, 9.98, and 3.52, respectively, when compared with PBMC cells. RCME at concentrations of 125 µg/mL and 250 µg/mL, significantly (p < 0.05) decreased nitrite production in J774A.1 cells, when exposed to lipopolysaccharide. This study demonstrated that RCME showed significant cytotoxic activity against HEP-G2 and L5178Y-R cells, without affecting normal VERO, PBMC, and J774A.1 cells.
Synthesis and Characterization of Poly(DL-lactide) Containing Fluorene Structures
Authors: Yu Chung-Fu , Rwei Syang-Peng , Yang Shung-Jim , Tsen Wen-Chin , Lin Li-Huei
Published: June 2023

9,9-bis[4-(2-hydroxy-3-acryloyloxypropoxy)phenyl]fluorene (BPF) hydroxyl groups (-OH) were used as initiators in the ring-opening polymerization reaction with DL-lactide monomers at different molar ratios to synthesize a Poly(DL-lactide) polymer containing bisphenol fluorene structure and acrylate functional groups (DL-BPF). The polymer’s structure and molecular weight range were analyzed using NMR (1H, 13C) and gel permeation chromatography. DL-BPF was then subjected to photocrosslinking using the photoinitiator Omnirad 1173, resulting in the formation of an optically transparent crosslinked polymer. Characterization of the crosslinked polymer involved analyzing its gel content, refractive index, thermal stability (via differential scanning thermometry (DSC) and thermogravimetric analysis (TGA)), as well as conducting cytotoxicity tests. The crosslinked copolymer exhibited a maximum refractive index of 1.5276, a maximum glass transition temperature of 61.1 °C, and cell survival rates higher than 83% in the cytotoxicity tests.
Development of a Water Transmission Rate (WTR) Measurement System for Implantable Barrier Coatings
Authors: Buchwalder Sébastien , Nicolier Cléo , Hersberger Mario , Bourgeois Florian , Hogg Andreas , Burger Jürgen
Published: June 2023

While water vapor transmission rate (WVTR) measurement is standardly used to assess material permeability, a system able to quantify liquid water transmission rate (WTR) measurement is highly desirable for implantable thin film barrier coatings. Indeed, since implantable devices are in contact or immersed in body fluids, liquid WTR was carried out to obtain a more realistic measurement of the barrier performance. Parylene is a well-established polymer which is often the material of choice for biomedical encapsulation applications due to its flexibility, biocompatibility, and attractive barrier properties. Four grades of parylene coatings were tested with a newly developed permeation measurement system based on a quadrupole mass spectrometer (QMS) detection method. Successful measurements of gas and water vapor and the water transmission rates of thin parylene films were performed and validated, comparing the results with a standardized method. In addition, the WTR results allowed for the extraction of an acceleration transmission rate factor from the vapor-to-liquid water measurement mode, which varies from 4 to 4.8 between WVTR and WTR. With a WTR of 72.5 µm g m−2 day−1, parylene C displayed the most effective barrier performance.
The Religious Genesis of Conspiracy Theories and Their Consequences for Democracy and Religion: The Case of QAnon
Authors: Roche Cárcel Juan Antonio
Published: June 2023

Here, we will approach Conspiracy Theories (CTs) and, specifically, QAnon following the three traditional sociological fields of research. After an introduction in which we contextualise CDTs socially, culturally, economically and politically and in which we establish a conceptual map of what they mean, on the historical level (1), we will clarify their religious genesis, through the main analogies between them, magic and religion and their practices and rituals, as well as the conversion of conspiratorial agents into social agents of a religious nature. On the analytical side (2), we will deal with the QAnon belief system. Finally (3), from a critical perspective, we will describe the causes and harmful consequences of QAnon, both for religious sentiment itself and for democracy. We will conclude by pointing out that QAnon affects the coherence and stability of religious beliefs and democracy; in fact, it can be seen as libertarian authoritarianism and populism, advocating a sick freedom, the ultimate expression of the modern feeling of individual powerlessness and of a Modernity that has failed to deliver on its promises.
The Digital Media Sermon: Definitions, Evaluations, Considerations
Authors: O’Lynn Rob
Published: June 2023

The question driving this particular paper is how to understand the place of the sermon on the digital frontier. In hopes of accomplishing this rather abstract task, this essay will begin with an analysis of the concept of digital rhetoric as articulated by Richard Lanham, Douglas Eyman, and Angela Haas. Next, this paper will review the dissertation literature published since 2013 that address preaching and technology. Then, this essay will address the “best practices” research on preaching and technology from Tripp Hudgins, MichaelChan, Sunggu Yang, Casey Sigmon, and Joshua Schatzle published since 2019 to see what influence the dissertation research is having on functional conversations. Finally, a proposal for capturing the concept of “digital homiletics” will be articulated based on John McClure’s idea of theological invention.
Everyday Energy Information Literacy and Attitudes towards Energy-Related Decisions: Gender Differences among Finns
Authors: Keränen Teija , Enwald Heidi
Published: June 2023

Many household-level decisions are made in families and, in studies, gender has been found to be an important factor affecting both household energy consumption behavior and household-level decisions related to energy use. This article scrutinizes everyday energy information literacy (EEIL) based on qualitative data collected from Finnish households in 2018 and reflects the findings by the gender differences that emerged from the quantitative analysis. The data (n = 415) included Finnish households (n = 323) and the residents of Ii municipality (n = 92), a pioneering municipality striving for carbon neutrality. The results indicate that there are gender differences in the dimensions of EEIL. Scrutinizing the qualitative data revealed the nuances of the differences. The qualitative data brought depth to the analysis by deciphering examples of respondents’ views and perceived challenges in improving energy efficiency at home. The examples also illustrate the respondents’ need for energy counselling and trusted parties from whom they hope information and advice. The article provides new information on gender differences in EEIL. Gaining more information on different groups and their attitudes, capabilities, and preferences helps to achieve carbon neutrality targets as a society. The results may be utilized in tailored communication for specific target groups and in communities’ decision making and policies.
Characteristics of Internal Solitary Waves in the Timor Sea Observed by SAR Satellite
Authors: Zhang Yunxiang , Hong Mei , Zhang Yongchui , Zhang Xiaojiang , Cai Jiehua , Xu Tengfei , Guo Zilong
Published: June 2023

Internal solitary waves (ISWs) with features such as large amplitude, short period, and fast speed have great influence on underwater thermohaline structure, nutrient transport, and acoustic signal propagation. The characteristics of ISWs in hotspot areas have been revealed by satellite images combined with mooring observation. However, the ISWs in the Timor Sea, which is located in the outflow of the ITF, have not been studied yet and the characteristics are unrevealed. In this study, by employing the Synthetic Aperture Radar (SAR) images taken by the Sentinel-1 satellite from 2017 to 2022, the temporal and spatial distribution characteristics of ISWs in the Timor Sea are analyzed. The results show that most of ISWs appear in Bonaparte basin and its vicinity. The average wavelength of the ISWs is 248 m, and most of the wave lengths are less than 400 m. The peak line of ISWs is longer in deeper water. The underwater structures of two typical ISWs are reconstructed based on the Korteweg–de Vries (KdV) equation combined with mooring observation. This shows that, compared with the two-layer model, the continuous layered model is more suitable for reconstructing the underwater structures of ISWs. Further analysis shows that both the rough topography and the spring-neap tides contribute to the generation of ISWs in the Timor Sea. This study fills a gap in knowledge of ISWs in regional seas, such as the Timor Sea.
Hyperspectral Remote Sensing Images Feature Extraction Based on Spectral Fractional Differentiation
Authors: Liu Jing , Li Yang , Zhao Feng , Liu Yi
Published: June 2023

To extract effective features for the terrain classification of hyperspectral remote-sensing images (HRSIs), a spectral fractional-differentiation (SFD) feature of HRSIs is presented, and a criterion for selecting the fractional-differentiation order is also proposed based on maximizing data separability. The minimum distance (MD) classifier, support vector machine (SVM) classifier, K-nearest neighbor (K-NN) classifier, and logistic regression (LR) classifier are used to verify the effectiveness of the proposed SFD feature, respectively. The obtained SFD feature is sent to the full connected network (FCN) and 1-dimensionality convolutional neural network (1DCNN) for deep-feature extraction and classification, and the SFD-Spa feature cube containing spatial information is sent to the 3-dimensionality convolutional neural network (3DCNN) for deep-feature extraction and classification. The SFD-Spa feature after performing the principal component analysis (PCA) on spectral pixels is directly connected with the first principal component of the original data and sent to 3DCNNPCA and hybrid spectral net (HybridSN) models to extract deep features. Experiments on four real HRSIs using four traditional classifiers and five network models have shown that the extracted SFD feature can effectively improve the accuracy of terrain classification, and sending SFD feature to deep-learning environments can further improve the accuracy of terrain classification for HRSIs, especially in the case of small-size training samples.
Multi-Scale Feature Interaction Network for Remote Sensing Change Detection
Authors: Zhang Chong , Zhang Yonghong , Lin Haifeng
Published: June 2023

Change detection (CD) is an important remote sensing (RS) data analysis technology. Existing remote sensing change detection (RS-CD) technologies cannot fully consider situations where pixels between bitemporal images do not correspond well on a one-to-one basis due to factors such as seasonal changes and lighting conditions. Existing networks construct two identical feature extraction branches through convolution, which share weights. The two branches work independently and do not merge until the feature mapping is sent to the decoder head. This results in a lack of feature information interaction between the two images. So, directing attention to the change area is of research interest. In complex backgrounds, the loss of edge details is very important. Therefore, this paper proposes a new CD algorithm that extracts multi-scale feature information through the backbone network in the coding stage. According to the task characteristics of CD, two submodules (the Feature Interaction Module and Detail Feature Guidance Module) are designed to make the feature information between the bitemporal RS images fully interact. Thus, the edge details are restored to the greatest extent while fully paying attention to the change areas. Finally, in the decoding stage, the feature information of different levels is fully used for fusion and decoding operations. We build a new CD dataset to further verify and test the model’s performance. The generalization and robustness of the model are further verified by using two open datasets. However, due to the relatively simple construction of the model, it cannot handle the task of multi-classification CD well. Therefore, further research on multi-classification CD algorithms is recommended. Moreover, due to the high production cost of CD datasets and the difficulty in obtaining them in practical tasks, future research will look into semi-supervised or unsupervised related CD algorithms.
Object-Oriented Clustering Approach to Detect Evolutions of ENSO-Related Precipitation Anomalies over Tropical Pacific Using Remote Sensing Products
Authors: Li Lianwei , Zhang Yuanyu , Xue Cunjin , Zheng Zhi
Published: June 2023

Precipitation extremes driven by the El Niño–Southern Oscillation (ENSO) are one of the critical ways in which the ENSO impacts the global climate, specifically in the tropical Pacific, where they have the potential to cause extreme weather conditions. However, existing approaches struggle to effectively identify the evolution of ENSO-related precipitation anomalies that change rapidly in spatial distribution. To address this challenge, we propose the object-oriented spatiotemporal clustering approach using remote sensing products (OSCAR) for detecting evolutions of ENSO-related precipitation anomalies. The OSCAR was validated using simulated datasets and applied to precipitation anomalies over the tropical Pacific. The simulation experiment demonstrates that the OSCAR outperforms the dual-constraint spatiotemporal clustering approach (DcSTCA) in accuracy, particularly for rapidly evolving precipitation anomaly variations. The application of the OSCAR demonstrates its ability to recognize the evolution of ENSO-related precipitation anomalies over the tropical Pacific, which may offer valuable references for global climate change research.
Creation and Verification of a High-Resolution Multi-Parameter Surface Meteorological Assimilation Dataset for the Tibetan Plateau for 2010–2020 Available Online
Authors: Wen Xiaohang , Zhu Xian , Li Maoshan , Chen Mei , Zhang Shaobo , Yang Xianyu , Zheng Zhiyuan , Qin Yikun , Zhang Yu , Lv Shihua
Published: June 2023

The Qinghai–Tibet Plateau (QTP) is a crucial component of the global climate system, influencing the regional and global climate through complex thermal and dynamic mechanisms. The high-altitude region, which is the largest part of the extra-polar cryosphere, encompasses extensive mountain glaciers, permafrost, and seasonally frozen land, making it highly sensitive to global climate change. However, the challenging environmental conditions, such as the harsh terrain and high altitude, coupled with sparse weather station distribution and weak observatory representation, make it difficult to accurately quantify the atmospheric conditions and land–atmosphere coupling systems and their effects on the surrounding areas. To address these challenges, we utilized the Weather Research and Forecasting (WRF) model and a three-dimensional variational (3DVAR) assimilation method to create a high-resolution assimilated dataset (HRAD). The QTP-HRAD, covering the spatial range of 70 to 110°E and 25 to 40°N, was validated using both surface weather station observations and the European Center for Medium-Range Weather Forecasts Reanalysis V5, and can now be utilized for further studies on land–atmosphere interactions, water cycling and radiation energy transfer processes, and extreme weather events in the region.