bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating cognitive flexibility deficit in schizophrenia using task-based whole-brain functional connectivity
Authors: Wang Yanqing , Hu Xueping , Li Yilu
Published: November 2022

Background

Cognitive flexibility is a core cognitive control function supported by the brain networks of the whole-brain. Schizophrenic patients show deficits in cognitive flexibility in conditions such as task-switching. A large number of neuroimaging studies have revealed abnormalities in local brain activations associated with deficits in cognitive flexibility in schizophrenia, but the relationship between impaired cognitive flexibility and the whole-brain functional connectivity (FC) pattern is unclear.

Method

We investigated the task-based functional connectivity of the whole-brain in patients with schizophrenia and healthy controls during task-switching. Multivariate pattern analysis (MVPA) was utilized to investigate whether the FC pattern can be used as a feature to discriminate schizophrenia patients from healthy controls. Graph theory analysis was further used to quantify the degrees of integration and segregation in the whole-brain networks to interpret the different reconfiguration patterns of brain networks in schizophrenia patients and healthy controls.

Results

The results showed that the FC pattern classified schizophrenia patients and healthy controls with significant accuracy. Moreover, the altered whole-brain functional connectivity pattern was driven by a lower degree of network integration and segregation in schizophrenia, indicating that both global and local information transfers at the entire-network level were less efficient in schizophrenia patients than in healthy controls during task-switching processing.

Conclusion

These results investigated the group differences in FC profiles during task-switching and not only elucidated that FC patterns are changed in schizophrenic patients, suggesting that task-based FC could be used as a potential neuromarker to discriminate schizophrenia patients from healthy controls in cognitive flexibility but also provide increased insight into the brain network organization that may contribute to impaired cognitive flexibility.
Protocol of a randomized controlled trial to investigate the efficacy and neural correlates of mindfulness-based habit reversal training in children with Tourette syndrome
Authors: Li Yanlin , Yan Junjuan , Cui Linyu , Chu Jiahui , Wang Xianbin , Huang Xi , Li Ying , Cui Yonghua
Published: November 2022

Background

Tourette syndrome (TS) is a developmental neuropsychiatric disorder. Behavior therapy, especially habit reversal training (HRT), has gradually become regarded as one of the core therapies for TS. Mindfulness approaches can improve psychological adjustment and reduce stress and anxiety, suggesting potential benefits when incorporated into behavior therapy. To improve the efficacy of HRT, we combined it with mindfulness, an approach named mindfulness-based habitual reversal training (MHRT). The aim of this protocol is to investigate the efficacy and neural mechanisms of MHRT for TS.

Methods/design

We will perform a randomized control trial (RCT) to evaluate the efficacy and neural mechanisms of MHRT. The sample will include 160 participants (including 120 patients with TS and 40 healthy controls). The patient sample will be randomly divided into three groups exposed to three different types of training: MHRT, HRT, and psychoeducation and supportive therapy (PST). Participants will be assessed and undergo resting-state fMRI scans at baseline and at the end of the 12-week training. The Yale Global Tic Severity Scale (YGTSS) and Premonitory Urge for Tic Scale (PUTS) will be used to assess the severity of tic symptoms and premonitory urges. The primary outcomes are change scores on the YGTSS and other assessments from baseline and the end of the training. The secondary outcomes are the neural correlates of these trainings among these groups based on graph theory, which is used to characterize brain functional connectivity networks. The default mode network (DMN) and the salience network (SN) will be assessed (which have been associated with mindfulness as well as the generation of tic symptoms) by network parameters, including clustering coefficients and shortest path lengths. Changes in these network parameters will be regarded as the neural correlates of the behavioral training.

Discussion

MHRT was newly developed for the treatment of TS. MHRT may lead to greater reductions in tic severity than traditional HRT. Changes in the network parameters of the DMN and SN may show associations with the efficacy of MHRT.

Clinical trial registration

http://www.chictr.org.cn, ChiCTR2100053077, China.
Knowledge, attitudes, and practices toward tuberculosis among Jordanian university students
Authors: Abu-Humaidan Anas H. A. , Tarazi Alaa , Hamadneh Yazan , Al-leimon Ahmad , Al-leimon Obada , Aljahalin Mohammad , Ahmad Fatima , Awajan Dima , Alaridah Nader
Published: November 2022

Background

Tuberculosis (TB) is one of the leading causes of death from infectious diseases worldwide with numerous undiagnosed and untreated cases, emphasizing the need for TB awareness to minimize transmission and initiate early treatment. Data regarding the knowledge, attitudes, and practices (KAP) toward TB among Jordanians is lacking but requires attention given the massive migration spells to Jordan from neighboring countries in the past decade.

Methods

A descriptive cross-sectional study was conducted from May to June 2022. An online questionnaire was developed following World Health Organization (WHO) recommendations for TB KAP surveys and was distributed to Jordanian university students. The questionnaire documented sociodemographic data and measured participants' KAP toward TB. Descriptive and analytic statistics were used to report KAP levels and highlight relevant sociodemographic factors associated with better KAP.

Results

602 participants completed the survey; most were females (60.8%), in their first 3 years of school (84.4%), and from a healthcare field of study (57.0%). The knowledge section median score was 27 out of 51. Knowledge gaps in TB treatment, and to a lesser extent, TB transmission routes were identified. The attitudes section median score was 6 out of 9, attitudes were generally positive toward TB patients with no indication of a social stigma. The practice section median score was 6 out of 8, most participants would take the correct measures if they suspected being infected, yet around 41.0% were not confident that masks are important in preventing airborne diseases. Students in healthcare specialties had significantly better KAP scores and identifying as a smoker was associated with a lower practice score.

Conclusion

Although university students displayed satisfactory KAP scores, the focus should be aimed at informing students from non-healthcare fields on TB transmission routes, treatment options, and the role of masks in preventing disease transmission.
Performance measures to benchmark the grasping, manipulation, and assembly of deformable objects typical to manufacturing applications
Authors: Kimble Kenneth , Albrecht Justin , Zimmerman Megan , Falco Joe
Published: November 2022

The National Institute of Standards and Technology is developing performance tests and associated artifacts to benchmark research in the area of robotic assembly. Sets of components consistent with mechanical assemblies including screws, gears, electrical connectors, wires, and belts are configured for assembly or disassembly using a task board concept. Test protocols accompany the task boards and are designed to mimic low-volume, high-mixture assembly challenges typical to small and medium sized manufacturers. In addition to the typical rigid components found in assembled products, the task boards include many non-rigid component operations representative of wire harness and belt drive assemblies to support research in the area of grasping and manipulation of deformable objects, an area still considered to be an emerging research problem in robotics. A set of four primary task boards as well as competition task boards are presented as benchmarks along with scoring metrics and a method to compare robot system assembly times with human performance. Competitions are used to raise awareness to these benchmarks. Tools to progress and compare research are described along with emphasis placed on system competition-based solutions to grasp and manipulate deformable task board components.
Heavy metals and metalloids exposure and in vitro fertilization: Critical concerns in human reproductive medicine
Authors: Obasi Cecilia Nwadiuto , Frazzoli Chiara , Orisakwe Orish Ebere
Published: November 2022

Exposures to heavy metals and metalloids have been associated with decreased fecundity and fertility in couples conceiving via assisted reproduction. Heavy metals and metalloids can alter the homeostasis of critical hormones controlling sexual maturation by binding to critical hormones and receptors. This may disrupt the time course of sexual maturation directly or indirectly affecting reproductive competence in males and females. The present review aims to provide a summarized overview of associations between heavy metal exposure, reproductive concerns, and IVF outcomes. A systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) in Google Scholar, Scopus, EMBASE and PubMed databases. Initial search produced 1,351 articles from which 30 articles were eligible to be included in the systematic review. From our results, 16 articles reported associations between selected heavy metals and IVF outcomes, while 14 articles summarized the role of heavy metals in reproductive concerns. For the studies on IVF outcomes, different human samples were examined for heavy metals. Heavy metals and metalloids (Pb, Hg, Cd, Cr, Mn, As) correlated negatively with oocyte fertilization/pregnancy rates in hair, follicular fluid, serum, urine and seminal plasma samples, while Cd and Hg in whole blood samples showed no associations. For the studies on reproductive concerns, high levels of heavy metals/metalloids were implicated in the following conditions: infertility (Cd, Pb, Ba, U), spontaneous abortion/miscarriage (Pb, Cd, Sb), congenital heart disease (Al, Mg, Cd), PCOS (As, Cd, Hg, Pb), endometriosis (Pb) and uterine leiomyomata (Hg). Taken together, the results of our study suggest that the impact of heavy metals and metalloids exposure on reproductive health may contribute to the failure rates of in vitro fertilization.
Peri-urban agrifood systems and a landscape project in the southern Mediterranean. The case of the urban agglomeration of Sousse (Tunisia)
Authors: Hamrita Abdelkarim , Jiménez Daniel Ferrer , Rejeb Hichem
Published: November 2022

Peri-urban agrifood systems are often subject to urban pressure and other territorial factors, which justifies a detailed study and analysis from multiple points of view. The main objective of this work is to analyze the periurban agri-food systems of the urban agglomeration of Sousse (Tunisia), one of the most important in the country, from a landscape perspective. This work adds to the literature on policies and strategies for the planning and development of Tunisian cities, which are currently facing several environmental and social challenges, of which climate change, food security and sustainable development are the most important. This research focuses, as mentioned above, on the case of the urban agglomeration of Sousse (Tunisia), where the concurrence of different factors (political, environmental, economic, etc.), together with clearly deficient regulations and uncontrolled urban development, is threatening the survival of a traditional, largely family-based agricultural model, based on a mixed or polyculture production system, with olive groves predominating. The latter is vital to ensure the economic maintenance of many families and to actively contribute to food security, with the consequent production of varied, fresh, and quality food. In this sense, we present a specific method that, based on the integrative and strategic significance of landscapes, and on the Landscape Character Assessment methodology, employs the so-called “landscape project” as a tool to strengthen the resilience and sustainability of peri-urban agriculture and to prevent the loss of a landscape heritage that has always been considered crucial for the identity of the local population. The document highlights the importance of the concept of green infrastructure, which, when properly integrated into land-use planning instruments, can contribute to enhancing local and sustainable agri-food systems. It also points out the need to promote Agricultural Parks, a protected status already recognized in Spain and other countries, as instruments for management, promotion, and development, and puts forward some specific proposals for the enhancement and activation of peri-urban agricultural landscapes in the Sousse conurbation. In relation to this last aspect, it insists on the need to modify the current forms of governance, which would require greater leadership and involvement of the main actors in peri-urban agriculture.
Cloud-based cross-platform collaborative augmented reality in flutter
Authors: Carius Lars , Eichhorn Christian , Rudolph Linda , Plecher David A. , Klinker Gudrun
Published: November 2022

Augmented Reality (AR) as a technology in the business area is utilized in new frontiers such as collaborative real-time experiences and cloud-based solutions. However, there is still a strong tendency towards game engines, which hinders widespread adoption for businesses. We present a collaborative AR framework (Flutter plugin) aimed at lowering the entry barriers and operating expenses of AR applications. A cross-platform and cloud-based solution combined with a web-based content management system (cloud) is a powerful tool for non-technical staff to take over operational tasks such as providing 3D models or moderating community annotations. To achieve cross-platform support, the AR Flutter plugin builds upon ARCore (Android) and ARKit (iOS) and unifies the two frameworks using an abstraction layer written in Dart. In this extensive description we present an in-depth summary of the concepts to realize the framework and prove its performance being on the same level as the native AR frameworks. This includes application-level metrics like CPU and RAM consumption and tracking-level qualities such as keyframes per second used by the underlying SLAM algorithm, detected feature points, and area of tracked planes. Our contribution closes a gap in today’s technological landscape by providing an AR framework with the familiar development process of cross-platform apps. Building upon on a content management system (cloud) and AR can be a game changer to achieve business objectives, while being not restrained to stand-alone single-purpose apps. This will trigger a potential paradigm shift for previously complex-to-realize applications relying on AR, e.g., in production and planning. The AR Flutter plugin is fully open-source, the code can be found at: https://github.com/CariusLars/ar_flutter_plugin.
A proposed methodology for trip recovery training without a specialized treadmill
Authors: Lee Youngjae , Alexander Neil B. , Madigan Michael L.
Published: November 2022

Falls are the leading cause of accidental injuries among adults aged 65 years and older. Perturbation-based balance training is a novel exercise-based fall prevention intervention that has shown promise in reducing falls. Trip recovery training is a form of perturbation-based balance training that targets trip-induced falls. Trip recovery training typically requires the use of a specialized treadmill, the cost of which may present a barrier for use in some settings. The goal of this paper is to present a methodology for trip recovery training that does not require a specialized treadmill. A trial is planned in the near future to evaluate its effectiveness. If effective, non-treadmill trip recovery training could provide a lower cost method of perturbation-based balance training, and facilitate greater implementation outside of the research environment.
Higher-Order Curvatures of Plane and Space Parametrized Curves
Authors: Crasmareanu Mircea
Published: November 2022

We start by introducing and studying two sequences of curvatures provided by the higher-order derivatives of the usual Frenet equation of a given plane curve C. These curvatures are expressed by a recurrence starting with the pair (0,−k) where k is the classical curvature function of C. Moreover, for the space curves, we succeed in introducing three recurrent sequences of curvatures starting with the triple (−k,0,τ). Some kinds of helices of a higher order are defined.
An Observer-Based Stiffness Estimation for Space Target Capture by Space Robot with Controllable Damping Mechanism
Authors: Chang Rui , Jia Qingxuan , Chu Ming , Zhang Xiaodong
Published: November 2022

The space target capturing task using the spacecraft-manipulator system (SMS) has special significance in on-orbit servicing due to its theoretical challenges and practical value. The contact force between the end effector (gripper) and the target exerted by the tumbling motion of the space target destabilizes the spacecraft base. A full-dimensional controllable damping mechanism (FDCDM) with a cross-axis structure was designed to buffer the transient impact force on the end joint. The introduction of a damping mechanism gives the space robot a variable stiffness and damping system, and a stiffness estimation algorithm is proposed to calibrate the system stiffness, as stiffness cannot be measured directly. The full-dimensional controllable damping mechanism (FDCDM) with a cross-axis structure is equivalent to a four-DOF tandem joint, and the whole-body dynamic model of the SMS endowed with a full-dimensional controllable damping mechanism (FDCDM) was established using the Kane equation. Then, an unknown input observer (UIO)-based identification theory is proposed to precisely estimate the internal flexibility torque and the corresponding joint stiffness. A model-based neural learning algorithm is proposed to update the variable parameter matrix of the observer. The simulation experiment results demonstrate that the flexibility torque and joint stiffness could be accurately estimated within the expected error, illustrating the feasibility and effectiveness of the proposed method.
Dynamic Numerical Simulation of Hybrid Rocket Motor with HTPB-Based Fuel with 58% Aluminum Additives
Authors: Tian Hui , Meng Xiangyu , Zhu Hao , Li Chengen , He Lingfei , Cai Guobiao
Published: November 2022

The addition of aluminum (Al) to the fuel is an effective way to increase the regression rate of hybrid rocket motors (HRMs). Due to its high regression rate, the impact of the regression of combustion surface on the performance of HRMs cannot be ignored. Therefore, it is significant to establish a dynamic numerical simulation model to predict the performance of HRMs. In this study, the dynamic simulation model was established based on dynamic mesh technology and was verified by a firing test. The results show that the simulation results agree well with the experimental results, and the errors of the average thrust and combustion chamber pressure are 3.4% and 1.4%, respectively. The dynamic simulation shows that with the regression of the combustion surface, the vortex of the pre-combustion chamber is divided into two vortices. The vortex near the front of the grain will increase the regression rate downstream. The results show that the addition of Al can obviously improve the regression rate of HRMs. The fuel containing 58% Al can improve the regression rate by 88.8% compared with the fuel with pure hydroxyl-terminated polybutadiene (HTPB). Moreover, due to the higher combustion temperature and the scouring of metal particles, the ablation rate of the nozzle with carbon ceramic materials reaches 0.16 mm/s. This investigation provides a valuable reference for HRMs design and simulation.
Deep Muti-Modal Generic Representation Auxiliary Learning Networks for End-to-End Radar Emitter Classification
Authors: Zhu Zhigang , Yi Zhijian , Li Shiyao , Li Lin
Published: November 2022

Radar data mining is the key module for signal analysis, where patterns hidden inside of signals are gradually available in the learning process and its superiority is significant for enhancing the security of the radar emitter classification (REC) system. Owing to the disadvantage that radio frequency fingerprinting (RFF) caused by the imperfection of emitter’s hardware is difficult to forge, current deep-learning REC methods based on deep-learning techniques, e.g., convolutional neural network (CNN) and long short term memory (LSTM) are difficult to capture the stable RFF features. In this paper, an online and non-cooperative multi-modal generic representation auxiliary learning REC model, namely muti-modal generic representation auxiliary learning networks (MGRALN), is put forward. Multi-modal means that multi-domain transformations are unified to a generic representation. After this, the representation is employed to facilitate mining the implicit information inside of the signals and to perform the better model robustness, which is achieved by using the available generic genenation to guide the network training and learning. Online means the learning process of REC is only once and the REC is end-to-end. Non-cooperative denotes no demodulation techniques are used before the REC task. Experimental results on the measured civil aviation radar data demonstrate that the proposed method enables one to achieve superior performance.
Impact of Phosphorous Fertilization on Rape and Common Vetch Intercropped Fodder and Soil Phosphorus Dynamics in North China
Authors: Qu Jiahui , Li Lijun , Zhao Peiyi , Han Dongyu , Zhao Xinyao , Zhang Yanli , Han Li , Wang Ying
Published: November 2022

This study explores the effect of phosphorus (P) fractions, under P addition or not, based on a common vetch-rape model cropping system in alkaline soil. A two year field experiment was conducted at Tuzuo Banner modern agricultural Park in Inner Mongolia, China. Two phosphorus levels, including P0 (no fertilizer) and P45 (45 kg·ha−1 P), were performed in common vetch and rape either grown alone or intercropped. We analyzed the changes of the physicochemical properties and phosphorus fractions in the rhizosphere soil. Intercropping enhanced the common vetch and rape yield by 42.05% and 24.91%, on average, compared with corresponding sole cropping on an equivalent area basis. The average land equivalent ratio (LER) was 1.34. Intercropping had a significant AP concentration, of 65.32% and 33.99% at the P0 level, and 62.83% and 36.19% at the P45 level, respectively, compared to that of the sole common vetch and rape. With the application of P, intercropping improved the Resin-Pi and NaHCO3-Pi fraction (61.17%, 87.03% at the P0 level and 96.50%, 41.85% at the P45 level, compared to monocropped common vetch and rape in 2019). The changes in NaOH-Pi and NaOH-Po (except for NaOH-Pi in 2019) showed no significant difference between cropping systems. Intercropping significantly accumulated concentrations of HCl-P, while depleting Residual-P, in 2020. In conclusion, common vetch/rape with the addition of P polyculture stimulated rhizosphere soil P mobilization and had a yield advantage over sole cropping.
Design and Simulation Analysis of a Reverse Flexible Harvesting Device for Fresh Corn
Authors: Zhang Hongmei , Chen Bo , Li Zhijie , Zhu Chenhui , Jin E , Qu Zhe
Published: November 2022

Aiming at the problem of grain breakage during the harvesting of fresh corn, this paper theoretically analyzes the collision process between the ear picking device and the corn ear, and a flexible ear picking structure composed of flexible materials and buffer springs is determined. Combined with a new harvesting method that reverses the growth direction from top to bottom, a reverse flexible ear plucking device for fresh corn was designed. We used the ADAMS software to simulate the ear picking process of fresh corn, analyze the contact force between the rigid structure and flexible buffer structure under different picking claw speeds and stalk feeding speeds, and obtain the optimal parameter combination: the picking claw speed was 2 m/s, and the stalk feeding speed was 1 m/s. On the basis of the simulation, a reverse flexible fresh corn harvesting bench was built, and the optimal operating parameters were obtained from the test: the speed of ear picking claws was 2.11 m/s; the number of ear picking claws was four; the thickness of the flexible body was 4.52 mm; the stem feeding speed was 1.04 m/s; the corresponding grain breakage rate was 0.128%, which was far lower than the national standard (0.5%); and the ear impurity content was 0.3%, which was far lower than the national standard (2%). The results are consistent with the simulation results, proving that the model is reliable. This research achieved the harvest of fresh corn ears with a low grain damage rate, verified the possibility of reverse flexible ear picking, and provided a reference for the research and development of low-damage fresh corn harvesting machines.
Virulence Diversity of Puccinia striiformis f. sp. Tritici in Common Wheat in Russian Regions in 2019–2021
Authors: Gultyaeva Elena , Shaydayuk Ekaterina , Kosman Evsey
Published: November 2022

Yellow (stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major disease of common wheat worldwide. Disease epidemics in Russia have been frequent and destructive, mostly in the North Caucasus. However, over the last 5 years, the significance of Pst has markedly increased in other Russian regions. Therefore, the Pst virulence diversity was investigated in Triticum aestivum in six geographically distant regions of the European (North Caucasus, North-West, Low Volga, Central Black Earth region, and Volga-Vyatka) and Asian (West Siberia) parts of Russia, with strongly different climates, environmental conditions, and growing wheat genotypes. Seventy-nine virulence pathotypes among 117 isolates were identified using the 12 Avocet Yr gene lines (Yr1, Yr5, Yr6, Yr7, Yr8, Yr9, Yr10, Yr15, Yr17, Yr24, Yr27, and YrSp) and eight supplemental wheat differentials (Heines VII, Vilmorin 23, Hybrid 46, Strubes Dickkopf, Carstens V, Suwon 92/Omar, Nord Desprez, and Heines Peko). Only four pathotypes occurred in two or more regions. High variability was detected within Pst populations from Dagestan, Central, North-West, and West Siberia that postulated to form an intrapopulation subdivision of each of them into several subgroups. Most regional virulence groups of pathotypes were closely related, except for several small subgroups of pathotypes from West Siberia, Dagestan, North-West, and Central European regions. All Pst isolates were avirulent in lines with Yr5, Yr10, Yr15, and Yr24 genes. Virulence to Yr17 was detected for several isolates of two pathotypes, one each from the North-West and Low Volga regions. Variation in virulence frequency was observed in other differential lines.
Qualitative and Nutritional Evaluation of Paddlefish (Polyodon spathula) Meat Production
Authors: Simeanu Daniel , Radu-Rusu Răzvan-Mihail , Mintas Olimpia Smaranda , Simeanu Cristina
Published: November 2022

Polyodon spathula is a valuable species of sturgeon native to North America that has acclimatized very well in Europe. Detailed knowledge of the quantitative and qualitative productive performance of paddlefish meat is of interest. Through this article, we aimed to highlight the chemical composition, cholesterol, and collagen content of fillets issued from paddlefish aged two and three summers and to highlight, as well as the nutritional value, the profile of fatty acids and amino acids, the sanogenic indices and the biological value of proteins for the epaxial and hypaxial muscle groups. The chemical analysis of the fillets by age indicated slightly higher values in summer three, compared to summer two: +5.32% dry matter, +0.89% protein, +41.21% fat, therefore +10.94% gross energy and for collagen by 2.94%; instead, for water, minerals and the W/P ratio the values were lower by 1.52%, 10.08%, and 2.29%. The nutritional assessment revealed that paddlefish has a meat with high PUFA content (approx. 22% of total fatty acids) and good values of sanogenic indices (Polyunsaturation Index = 7.01–8.77; Atherogenic Index = 0.57; Thrombogenic Index = 0.38–0.39; Hypocholesterolemic Fatty Acids = 33.01–41.34; Hypocholesteromic/Hypercolesteromic Fatty Acids ratio = 1.9). Also, the proteins of these fish are of good quality for young and adult consumers (EAA index = 156.11; Biological Value = 158.46; Nutritional Index (%) = 28.30) and good enough for children (Essential Amino Acids Index = 96.41; Biological Value = 93.39; Nutritional Index (%) = 17.45).
Genetics, Genomics, and Breeding in Melon
Authors: Xu Longlan , He Yuhua , Tang Lingli , Xu Yongyang , Zhao Guangwei
Published: November 2022

Melon is an important horticultural crop worldwide. The high diversity of melon makes it a model plant for various different properties. Some quantitative trait loci or candidates have been discovered, but few were verified as limiting genetic transformation and genome editing systems. Identifying new genetic resources with resistance and special fruit quality traits is imperative to develop effective and useful breeding technologies in melon. This review describes the advances in genetics, genomics, and the breeding of melon and puts forward some recommendations in these areas.
Early Season Growth Responses of Resistant and Susceptible Cotton Genotypes to Reniform Nematode and Soil Potassium Application
Authors: Singh Bhupinder , Chastain Daryl R. , Stetina Salliana R. , Gardiner Emile S. , Snider John L.
Published: November 2022

A greenhouse study was conducted to investigate the roles that host plant resistance and soil potassium (K) levels play in affecting Rotylenchulus reniformis Linford and Oliveira (Tylenchida: Hoplolaimidae) (RN) populations and early season cotton (Gossypium hirsutum L.) growth. Two upland, RN-resistant cotton lines (G. barbadense introgressions: 08SS110-NE06.OP and 08SS100), a genetic standard (Deltapine 16) and a commercially available susceptible cultivar (PHY 490 W3FE) were evaluated at four different levels of K [100% of recommended rate, 150% of recommended, 50% of recommended, and a base level] from seeding until harvesting, 60 days after sowing (DAS). Quadratic functions (r2 = 0.82 to 0.95) best described the early season growth response of cotton genotypes to soil K. The base K level was associated with the lowest values for most morphological variables, including plant height (PH), mainstem nodes (MSN), leaf area, and dry weight at 30 DAS and 60 DAS. However, soil K did not affect RN population counts (RC). Additionally, soil K did not influence the rate of change in growth variables among genotypes. The resistant genotype 08SS110-NE06.OP showed greater growth in terms of time to first true leaf, PH, MSN, and above-ground dry weights compared to the commercially available susceptible genotype. No interaction between K and RN or genotype and RN was found in early season cotton growth. However, RC in pots of resistant genotypes was less than in pots of susceptible genotypes. Our research on the early season growth response to soil K by novel, RN-resistant genotypes and susceptible genotypes contributes to the development of improved RN resistance and fertilization management in cotton.
Simple and Rapid High-Performance Liquid Chromatography Method for Simultaneous Determination of Picloram and 2,4-D in Pesticide Formulations
Authors: Santilio Angela , Girolimetti Silvana , Picardo Valentina
Published: November 2022

Picloram and 2,4-D are systemic herbicides used to control a wide range of broad-leaved weeds and post-emergence annual and perennial broad-leaved weeds, respectively. The use of plant protection products containing Picloram and 2,4-D necessitates the development of analytical methods, useful for the laboratories focused on control, for monitoring. In this study, we designed and validated an analytical method for the rapid determination of picloram and 2,4-D by HPLC-DAD. The method involves the extraction of the substances by sonication of the sample with methanol, followed by dilution in acetonitrile, and direct injection on a liquid chromatography system, based on the use of a Gemini C18 column. We used an isocratic mobile elution consisting of acetonitrile and water acidified 1% with H3PO4 (50:50, v/v). We validated the proposed method, which demonstrated linearity within the concentration range of 0.01–0.028 mg/mL for picloram and 2,4-D, with a correlation coefficient (R2) of 0.9993 for picloram and 0.9999 for 2,4-D. We considered precision, repeatability and selectivity in the validation. The repeatability of the method expressed as percent of relative standard deviation (%RSD) was lower than 1% for both substances. The proposed method is suitable for the simultaneous determination of picloram and 2,4-D in pesticide formulations.
Effects of Feeding and Drinking Behavior on Performance and Carcass Traits in Beef Cattle
Authors: Pires Bianca V. , Reolon Henrique G. , Abduch Natalya G. , Souza Luana L. , Sakamoto Leandro S. , Mercadante Maria Eugênia Z. , Silva Rafael M. O. , Fragomeni Breno O. , Baldi Fernando , Paz Claudia C. P. , Stafuzza Nedenia B.
Published: November 2022

Feed and water efficiency are important traits to improve beef cattle production’s economic and environmental sustainability. This study evaluated residual feed intake (RFI) and residual water intake (RWI) and their relationship with performance, ingestive behavior, and carcass traits in Caracu beef cattle. The data were analyzed using a generalized linear model with least squares means. The ingestive behavior, performance, and carcass traits were influenced by sex (p < 0.05). Males showed higher dry matter intake (DMI), average daily gain (ADG), mid-test metabolic weight (BW0.75), rib eye area, and rump fat thickness than females, besides spending more time drinking and eating. Low RFI animals exhibited higher DMI than high RFI animals. Low RWI animals ingested 3.89 L/d of water further than high RWI animals. The interaction between sex and RWI influenced the DMI, BW0.75, and backfat thickness. The ingestive behavior of low and high RFI animals was similar, although high RWI animals visited a smaller number of drinkers than low RWI animals. Water intake positively affects productive efficiency, and the combined use of RWI and RFI may help improve the selection of more efficient animals contributing to reducing the costs of beef cattle production and improving environmental sustainability.
In Vitro Antimicrobial Activity of Piper betle Leaf Extract and Some Topical Agents against Methicillin-Resistant and Methicillin-Susceptible Staphylococcus Strains from Canine Pyoderma
Authors: Phensri Patcharaporn , Thummasema Kokaew , Sukatta Udomlak , Morand Serge , Pruksakorn Chantima
Published: November 2022

As multidrug-resistant methicillin-resistant staphylococci (MRS) is becoming more prevalent in canine pyoderma, the discovery of new therapeutic options is required. This study aimed to test the antimicrobial activity of crude Piper betle leaf extract and some topical antimicrobial agents against canine Staphylococcus clinical strains by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The results showed that the mean MICs of chlorhexidine, miconazole, crude P. betle leaf extract, azelaic acid, and benzoyl peroxide against Staphylococcus strains were 1.41, 1.62, 252.78, 963.49, and 1342.70 mg/L, respectively. Therefore, betel leaf extract demonstrated a superior efficacy to azelaic acid and benzoyl peroxide. Furthermore, the ratio of MBC/MIC of betel leaf extract was 1.75, indicating its bactericidal action. When applied to methicillin-resistant S. pseudintermedius (MRSP) and methicillin-susceptible S. pseudintermedius (MSSP), betel leaf extract was equally efficient towards both groups. S. pseudintermedius strains were more susceptible to betel leaf extract than S. schleiferi subsp. coagulans. In gas chromatography–mass spectrometry analysis, eugenol and hydroxychavicol appeared to be the major components of betel leaf extract. Given its efficacy, dogs with pyoderma could benefit from the use of betel leaf extract as a topical antimicrobial alternative.
Evaluation of Spray-Dried Bovine Hemoglobin Powder as a Dietary Animal Protein Source in Nile Tilapia, Oreochromis niloticus
Authors: Amer Shimaa A. , Farahat Mahmoud , Khamis Tarek , Abdo Samar A. , Younis Elsayed M. , Abdel-Warith Abdel-Wahab A. , Reda Rehab , Ali Sozan A. , Davies Simon J. , Ibrahim Rowida E.
Published: November 2022

The present study evaluated the potential effects of dietary inclusion of spray-dried bovine hemoglobin powder (SDBH) on the growth, gene expression of peptide and amino acid transporters, insulin growth factor-1 (IGF-1) and myostatin, digestive enzymes activity, intestinal histomorphology and immune status, immune-related gene expression, and economic efficiency in Nile tilapia, Oreochromis niloticus. Two hundred twenty-five fingerlings (32.38 ± 0.05 g/fish) were distributed into five treatments with five dietary inclusion levels of SDBH: 0, 2.5, 5, 7.5, and 10% for a ten-week feeding period. Dietary inclusion of SDBH linearly increased the final body weight (FBW), total weight gain (TWG), specific growth rate (SGR), and protein efficiency ratio (PER). Additionally, a linear decrease in feed conversion ratio (FCR) and daily feed intake relative to the daily BW was reported in the highest inclusion levels (7.5 and 10%). Dietary inclusion of SDBH was associated with a significant increase in the intestinal villous height (VH), villous width (VW), villous height: crypt depth ratio (VH: CD), and muscle coat thickness (MCT), with the highest values reported in SDBH7.5 group. Increased serum growth hormone levels and decreased serum leptin hormone levels were also reported by increasing the SDBH level. The serum glucose level was decreased in the SDBH7.5 and SDBH10 groups. The digestive enzymes’ activity (amylase and protease) was increased by increasing the SDBH inclusion level. An up-regulation in the expression of peptide and amino acid transporters, IGF-1, and down-regulation of myostatin was reported in the SDBH2.5 to SDBH7.5 groups. Spleen sections showed more lymphoid elements, especially in the SDBH2.5 and SDBH7.5 groups. The SDBH inclusion increased the serum lysozyme activity, nitric oxide (NO), and complement 3 (C3) levels, with the highest values recorded in the SDBH5 group. The phagocytic % and the phagocytic index were increased by increasing the SDBH inclusion %. The expressions of immune-related genes (transforming growth factor-beta (TGF-β), Toll-like receptor 2 (TLR2), and interleukin 10 (IL10)) were up-regulated by SDBH inclusion with the highest expression in the SDBH5 group. Economically, the feed costs and feed costs/kg gain were linearly decreased in the SDBH7.5 and SDBH10 diets. In conclusion, spray-dried bovine hemoglobin powder could be used as a protein source for up to 10% of the diets of Nile tilapia for better growth and immune status of fish.
Evaluation of Shiitake Mushroom (Lentinula edodes) Supplementation on the Blood Parameters of Young Thoroughbred Racehorses
Authors: Soroko-Dubrovina Maria , Górniak Wanda , Zielińska Paulina , Górniak Aleksander , Čebulj-Kadunc Nina , Korczyński Mariusz
Published: November 2022

The aim of this study was to evaluate the effect of shiitake mushroom (Lentinula edodes) supplementation on the hematology and biochemical blood parameters of young Thoroughbred racehorses. The study was conducted with 20 horses divided into two groups: the supplemented and the control group. The supplemented group was given 30 g of L. edodes daily for four months. One blood sample was collected four times from each horse at four-week intervals. The hematology analysis in the supplemented group showed a higher level of monocytes at day 56 when compared to the control group (p = 0.000986). Biochemical analysis showed that alkaline phosphatase is most sensitive to shiitake mushroom supplementation, with statistically significant lower levels in supplemented group compared to the control group on all individual days of blood sampling. It was also found that supplementation had an effect on the decrease of glucose levels on days 28 (p = 0.009109) and 56 y (p = 0.025749), on reduction aspartate aminotransferase level on day 56 (p = 0.017258) and a decrease of lactic acid on day 28 of sampling (p = 0.037636). Cholesterol levels decreased consistently in all individual days of blood sampling. Further studies are needed to show the influence of supplementation with shiitake mushroom in larger groups of horses over a longer period.
Transportation Stress Increases Fos Immunoreactivity in the Paraventricular Nucleus, but Not in the Nucleus of the Hippocampal Commissure in the Pekin Duck, Anas platyrhynchos domesticus
Authors: Tonissen Sara , Tetel Victoria , Fraley Gregory S.
Published: November 2022

Commercial poultry undergo transportation during their life, and the effects of transportation can negatively impact poultry production and welfare. In order to maintain physiological homeostasis, the hypothalamic–pituitary–adrenal axis (HPA) works to respond to stressors. Previous studies by others have shown contradictory effects of transportation on corticosterone release. However, recent studies from our lab and by others have shown that cortisol may also be an important hormone in the avian HPA. The purpose of our current study was to determine the effects of transportation stress on the stimulation of brain nuclei that regulate the HPA in birds, and on glucocorticoid (GC) secretion. To test this hypothesis, we collected blood and brain samples from developer drakes and hens (N = 10 per sex/time point): 24 h prior to transportation, immediately after transportation, 24 h after transportation, and 1 week after transportation. Serum GC levels and fos immunocytochemistry (ICC) within the nucleus of the hippocampal commissure (NHpC) and paraventricular nucleus (PVN) were measured. Data were analyzed using a two-way ANOVA. Post hoc analysis was completed using a Fisher’s PLSD with a p < 0.05 considered significant. We observed a sex difference (p < 0.05) in both corticosterone and cortisol secretion in Pekin ducks, although neither GC showed a significant increase in secretion associated with transportation. However, we did observe a significant (p < 0.05) increase in fos-like immunoreactivity for 24 h in the PVN, but not in the NHpC. Further studies are required to determine the specific role that GCs play in the avian stress response and the short-term stressors that could have long-term physiological effects on birds.
Revealing Genetic Diversity and Population Structure of Endangered Altay White-Headed Cattle Population Using 100 k SNP Markers
Authors: Liu Bo , Tao Weikun , Feng Donghe , Wang Yue , Heizatuola Nazigul , Ahemetbai Tenes , Wu Weiwei
Published: November 2022

Understanding the genetic basis of native cattle populations that have adapted to the local environment is of great significance for formulating appropriate strategies and programs for genetic improvement and protection. Therefore, it is necessary to understand the genetic diversity and population structure of Altay white-headed cattle so as to meet the current production needs under various environments, carry out continuous genetic improvement, and promote rapid adaptation to changing environments and breeding objectives. A total of 46 individual samples of endangered Xinjiang Altay white-headed cattle were collected in this study, including nine bulls and 37 cows. To collect genotype data, 100 k SNP markers were used, and then studies of genetic diversity, genetic structure, inbreeding degree, and family analysis were carried out. A total of 101,220 SNP loci were detected, and the genotype detection rate for individuals was ≥90%. There were 85,993 SNP loci that passed quality control, of which 93.5% were polymorphic. The average effective allele number was 0.036, the Polymorphism Information Content was 0.304 and the minimum allele frequency was 0.309, the average observed heterozygosity was 0.413, and the average expected heterozygosity was 0.403. The average genetic distance of Idengtical By State (IBS) was 0.3090, there were 461 ROH (genome-length homozygous fragments), 76.1% of which were between 1 and 5 MB in length, and the average inbreeding coefficient was 0.016. The 46 Altay white-headed cattle were divided into their families, and the individual numbers of each family were obviously different. To sum up, the Altay white-headed cattle conservation population had low heterozygosity, a high inbreeding degree, few families, and large differences in the number of individuals in each family, which can easily cause a loss of genetic diversity. In the follow-up seed conservation process, seed selection and matching should be carried out according to the divided families to ensure the long-term protection of Altay white-headed cattle genetic resources.
Effects of Dietary Oregano Essential Oil on Cecal Microorganisms and Muscle Fatty Acids of Luhua Chickens
Authors: Wu Tao , Yang Farong , Jiao Ting , Zhao Shengguo
Published: November 2022

This experiment was conducted to investigate the effects of oregano essential oil on the cecal microorganisms and muscle fatty acids of Luhua chickens. One hundred and twenty 49-day-old healthy dewormed Luhua chickens were randomly divided into four groups with three replicates per group and ten chickens per replicate. The corn–quinoa and soybean meal diets were supplemented with 0 (Q8 group), 50 (QO50 group), 100 (QO100 group) and 150 mg·kg−1 (QO150 group) of oregano essential oil, respectively, and the experiment lasted for 75 days. The composition of intestinal flora was detected by Illumina sequencing of the 16S rRNA V4 region, and the composition and content of fatty acids in the muscles were analyzed by gas chromatography. The results showed that dietary oregano essential oil can effectively increase the contents of elaidic acid (C18:ln9t), polyunsaturated fatty acids (PUFAs) and n-3 polyunsaturated fatty acids (n-3 PUFAs) in breast muscle tissues. However, the fatty acid composition and PUFA content in leg muscle tissues were not significantly improved. According to a 16S rRNA high-throughput sequencing analysis, dietary oregano essential oil supplementation with a certain concentration can change the cecal microbial community composition of broilers. At the phylum level, Elusimicrobia in the QO150 group was significantly lower than that in Q8 group (p < 0.05). At the genus level, Phascolarctobacterium, Parasutterella and Bilophila in the experimental groups (QO50, QO100 and QO150) were significantly lower than those in the Q8 group (p < 0.05). An enrichment analysis of the microbial function found that the amino acid metabolism, energy metabolism, metabolism, signal transduction and genetic information processing were mainly enriched in the experimental groups, which promoted the digestion and absorption of nutrients and enhanced intestinal barrier functioning. An analysis of the association between fatty acids and microbes found that the abundance of microbiota was significantly correlated with partially saturated fatty acids (SFAs) and unsaturated fatty acids (UFAs) (p < 0.05). In conclusion, the dietary addition of oregano essential oil can effectively improve cecal microbial community composition, promote the digestion and absorption of nutrients, and enhance intestinal barrier functioning. It can significantly improve the content of some fatty acids, and there was a certain correlation between caecum microorganisms and fatty acid deposition in muscles.