bluuebunny/crossref_metadata_2025 huggingface dataset:
Pensamento criativo na prática didática de professores que ensinam matemática
Authors: Góes Heliza Colaço , Guérios Ettiène Cordeiro
Published: January 2024

Este artigo apresenta resultados de pesquisa de doutorado cuja abordagem é qualitativa, tendo como participantes seis professores que ensinam matemática da educação básica de Curitiba e região metropolitana. O objetivo é apresentar, a partir das vozes dos participantes, a “criatividade” como um dos subsídios oriundos da prática didática com abordagem em geometria por meio do método da modelização. O amparo teórico está em Morin; Moraes; Brandt; Suanno; De La Torre; Góes; e Guérios et al. Evidencia-se o pensamento criativo como um dos elementos do pensamento complexo, presente na essência do professor que ensina matemática e, além disso, apresenta-se o “Ser criativo” como constructo emergente das relações e dos entrelaces dos subsídios “criatividade” e “criar e recriar”, essencial e parte constitutiva para uma formação complexa.
Constituição da ação criativa em Modelagem Matemática
Authors: Setti Elenice Josefa Kolancko , Vertuan Rodolfo Eduardo
Published: January 2024

Este artigo apresenta parte dos resultados de uma pesquisa qualitativa que objetivou compreender como se constitui a ação criativa de um grupo de estudantes de um curso de Licenciatura em Matemática de uma universidade pública do oeste paranaense, ao lidar com atividades de Modelagem no contexto da disciplina de Modelagem Matemática I[1]. A partir das transcrições de gravações de um grupo durante o desenvolvimento das atividades, selecionamos excertos que apresentavam indícios da constituição da ação criativa; em seguida, tecemos constatações e sínteses para a construção de categorias. Com base nas análises de uma das categorias, verificou-se que as ações criativas se constituem por intervenções e elementos que funcionam ora como gatilhos iniciais, ora como gatilhos de criação e ora como intervenções inibidoras.
Criatividade matemática com histórias em quadrinhos no primeiro ano do ensino fundamental
Authors: Elert Cristiane Winkel , Grützmann Thaís Philipsen
Published: January 2024

O ensino da Matemática de forma criativa, a partir da literatura infantil, é o tema deste artigo, que busca analisar uma proposta prática realizada em sala de aula com 24 alunos do primeiro ano do Ensino Fundamental. Os referenciais teóricos utilizados apresentam o ensino da Matemática através da literatura, especificamente das histórias em quadrinhos enquanto gênero textual para a criação e resolução de problemas pelos alunos, bem como a criatividade matemática nesse processo. Os resultados e análises se deram a partir da criação de seis tirinhas com situações-problema de adição (acrescentar e reunir) e subtração (retirar), e diferentes estratégias de resolução. Percebemos a criança como protagonista em um ambiente criativo e com domínio das habilidades matemáticas presentes nos registros gráficos, bem como a possibilidade da criatividade matemática.
Criatividade em matemática e o estágio supervisionado
Authors: Leal Márcia Rodrigues , Fontes Líviam Santana , Santos Cleiton Rodrigues dos , Gontijo Cleyton Hércules
Published: January 2024

A pesquisa objetiva analisar as percepções de docentes formadores em relação às práticas ocorridas no decorrer do Estágio Supervisionado em cursos de licenciatura em Matemática, no que se refere ao desenvolvimento do processo criativo do estudante. Trata-se de uma pesquisa de natureza qualitativa. Os sujeitos da investigação são professores de instituições de ensino superior públicas em Goiás e Distrito Federal e as informações foram analisadas mediante análise de conteúdo, constituindo-se o estudo de caso. Dentre os resultados obtidos, destaca-se que o termo criatividade é compreendido nas dimensões originalidade e flexibilidade de pensamento, sendo que a maioria dos docentes se identifica como um professor criativo e que reconhece o processo criativo no desenvolvimento de suas atividades. Segundo a pesquisa, o Estágio Supervisionado em Matemática é uma fase de fundamental importância no processo de formação docente e pode propiciar um ambiente de elevação do potencial criativo dos estudantes.
Criatividade na formação de professores que ensinam matemática
Authors: Marcatto Flávia Sueli Fabiani
Published: January 2024

Esta investigação faz parte de um projeto de pesquisa sobre o desenvolvimento do raciocínio matemático através de tarefas de resolução e proposição de problemas na perspectiva do Modelo Exploratório de Resolução de Problemas e apresenta resultados de uma experiência realizada na formação de professores que ensinam matemática. A estudo tem cunho qualitativo e interpretativo com base empírica, apoiada na pesquisa baseada em design. O objetivo principal é implementar tarefas instrucionais de resolução de problemas na formação de professores que ensinam matemática para a constituição de uma base de conhecimento sobre a resolução de problemas e a criatividade. A liberdade permitida aos professores e futuros professores, quer no desenvolvimento do raciocínio matemático, quer na comunicação matemática parece ter influência na promoção de resoluções matemáticas pessoais, inovadoras e criativas.
Equações Polinomiais do 1º Grau
Authors: Oliveira Raimunda de , Nogueira Cleia Alves
Published: January 2024

Este artigo apresenta resultados da análise de capítulos relacionados ao objeto de conhecimento “Equações Polinomiais do 1º grau”, nos livros didáticos de Matemática do 7º ano do Ensino Fundamental aprovados e distribuídos a instituições de ensino no Programa Nacional do Livro Didático (PNLD). O objetivo desta análise foi avaliar a potencialidade desses materiais presentes na maioria das salas de aula brasileiras para o estímulo da criatividade em Matemática. Foram analisados 12 livros didáticos, que compõem as coleções distribuídas no período de 2021/2024, de acordo com dados do Ministério da Educação (MEC). Concluímos que os exercícios contidos nos livros didáticos, em sua maioria, são caracterizados como problemas fechados que demandam processos cognitivos de baixa complexidade, como memorização e repetição de procedimentos.
Translanguaging as a Counter-Narrative in EFL Practice
Authors: Sugiharto Setiono
Published: January 2024

While concerns over inequalities of multilingualism – a real phenomenon in multilingual countries due to the positive attitudes toward English as a global language – should not be overlooked, there are occasions especially in a classroom context where multilingual speakers defy the exclusive use of English, and instead creatively mix the English language with their own mother tongues, resulting in translingual Englishes (Dovchin, Sultana & Pennycook, 2016). In this paper, I will show that despite the strict imposition of the English-only-policy in schools in Indonesia – a source of inequalities in learning and teaching in the country – both students and teachers manage to surreptitiously translanguage their interactions using varied linguistic codes for achieving successful communication in a class interaction. I see their translingual Englishes as a strategic practice initiated by the teachers to not only open up a space for them to reveal their real multilingual identities, but also to legitimize these identities. Finally, in teacher-dominated classrooms where students often keep silent and are unwilling to initiate a conversation and to argue over a controversial issue, translanguaging is a pedagogically useful practice for encouraging students to negotiate tensions that might occur in their effort to grapple with their learning of English. Thus, a focus on the ‘unequal’ in the classroom also leads to uncovering translingual spaces where efficient teaching and learning are facilitated, and multilingual identities affirmed.
Auxotrophy of Yersinia pestis Strains of Antiqua Biovar and Its Genetic Basis
Authors: Makashova M. A. , Kukleva L. M. , Chervyakova N. S. , Naryshkina E. A. , Kovrizhnikov A. V. , Eroshenko G. A. , Shvidenko I. G. , Kutyrev V. V.
Published: January 2024

The aim of the work was to study the growth dependence on amino acids in Yersinia pestis strains of different phylogenetic branches of the antiqua biovar and to determine the genetic basis of auxotrophy of these strains.

Materials and methods. We used 38 strains of Y. pestis, the main subspecies of the antiqua biovar, isolated in various foci of the world in the period of 1928–2020. The nutritional requirements of the strains were determined by incubation on Difco minimal agar with different sets of amino acids. Phylogenetic analysis of the strains was carried out using the Wombac 2.0 and SeaView 5.0.5 programs. Comparative analysis of the nucleotide sequences of the genes was performed using the BLAST algorithm and the Mega 7.0 program.

Results and discussion. The phylogenetic appurtenance of the investigated Y. pestis strains of antiqua biovar to the phylogenetic branches 0.ANT3, 0.ANT5, 1.ANT, 2.ANT3, 3.ANT2, 4.ANT has been determined. It is established that all studied strains have a common dependence of growth on three amino acids – phenylalanine, threonine, and methionine. In the majority of the strains of antiqua biovar of all phylogenetic branches, growth is also dependent on the presence of cysteine in the medium, except for a part of the strains of the phylogenetic branch 4.ANT. In 14 genes of sulfur and cysteine metabolism, 19 mutations have been identified. Each phylogenetic group of the antique biovar has a distinct profile of mutations in genes involved in cysteine biosynthesis. The leucine requirement of the strains belonging to phylogenetic branch 0.ANT5 has been established, possibly caused by a frame shift in the leuA gene. Strains of the 1.ANT branch isolated in the Democratic Republic of the Congo demonstrate an additional proline-dependent growth. The defined nutritional requirements in the strains and the genetic causes of auxotrophy complement the phenotypic and genetic characteristics of the phylogenetic branches of the antiqua biovar and can be used as genetic markers for the differentiation of these strains.
Cakrawala Di Bumi Nogosari Pengabdian Kepada Masyarakat Di Dusun Bulak Kunci Desa Nogosari Kecamatan Pacet
Authors: Iswanto Iswanto
Published: January 2024

Rasa syukur selalu tercurah kepada Allah SWT yang senantiasa melimpahkan rahmat, hidayah serta kesehatan, sehingga kami dapat melaksanakan program Kuliah Kerj Nyata Pencerahan (KKN-P) Universitas Muhammadiyah Sidoarjo tahun 2020. Kuliah Kerja Nyata (KKN) adalah program yang ditempuh mahasiswa Universitas Muhammadiyah Sidoarjo merupakan perwujudan dari salah satu Catur Dharma Perguruan Tinggi, yaitu bermaksud memberikan pengalaman secara langsung baik fisik maupun mental kepada calon sarjana dengan terjun bersama masyarakat dengan menerapkan ilmu jurusan masing-masing.
The Long-Lasting Exhumation History of the Ötztal-Stubai Complex (Eastern European Alps): New Constraints from Zircon (U–Th)/He Age-Elevation Profiles and Thermokinematic Modeling
Authors: Hölzer Kyra , Wolff Reinhard , Hetzel Ralf , Dunkl István
Published: January 2024

The Eastern European Alps formed during two orogenic cycles, which took place in the Cretaceous and Cenozoic, respectively. In the Ötztal-Stubai Complex—a thrust sheet of Variscan basement and Permo-Mesozoic cover rocks—the record of the first (Eoalpine) orogeny is well preserved because during the second (Alpine) orogeny, the complex remained largely undeformed. Here, new zircon (U–Th)/He (ZHe) ages are presented, and thermokinematic modeling is applied to decipher the cooling and exhumation histories of the central part of the Ötztal-Stubai Complex since the Late Cretaceous. The ZHe ages from two elevation profiles increase over a vertical distance of 1500 m from 56 ± 3 to 69 ± 3 Ma (Stubaital) and from 50 ± 2 to 71 ± 4 Ma (Kaunertal), respectively. These ZHe ages and a few published zircon and apatite fission track ages were used for inverse thermokinematic modeling. The modeling results show that the age data are well reproduced with a three-phase exhumation history. The first phase with relatively fast exhumation (~250 m/Myr) during the Late Cretaceous ended at ~70 Ma and is interpreted to reflect the erosion of the Eoalpine mountain belt. As Late Cretaceous normal faults occur at the margins of the Ötztal-Stubai Complex, normal faulting may have also contributed to the exhumation of the study area. Subsequently, a long period with slow exhumation (<10 m/Myr) prevailed until ~16 Ma. This long-lasting phase of slow exhumation suggests a rather low topography with little relief in the Ötztal-Stubai Complex until the mid-Miocene, even though the Alpine orogeny had already begun in the Eocene with the subduction of the European continental margin. Accelerated exhumation since the mid-Miocene (~230 m/Myr) is interpreted to reflect the erosion of the mountain belt due to the development of high topography in front of the Adriatic indenter and repeated glaciations during the Quaternary.
The Generalized AEP for Higher Order Nonhomogeneous Markov Information Source
Authors: Bai Lyu , Shi Zhiyan , Zhu Xiaoyu
Published: January 2024

The asymptotic equipartiton property (AEP) plays a crucial role in information theory, providing a theoretical foundation for understanding and analyzing aspects such as coding, compression, and the reliability of communication systems. In this paper, we mainly study the generalized AEP of higher order nonhomogeneous Markov information sources by establishing several strong deviation theorems. To achieve this, we first introduce the concepts of generalized sample divergence rate of th-order nonhomogeneous Markov information sources. Meanwhile, we give a class of generalized strong deviation theorems for moving average of the functions of + 1 variables for th-order nonhomogeneous Markov information sources, and also establish strong deviation theorems and strong limit theorems of the frequencies of occurrence of ordered tuples of states for this Markov information sources. Finally, the generalized AEP for th-order nonhomogeneous Markov information sources are establised. Our results also generalize some known results.
MSC(2020): 60J10; 60F15; 94A15
Deformation behaviour of concrete with different moisture contents subjected to compressive creep and cyclic loading
Authors: Kern Bianca , Podhajecky Anna-Lena , Lohaus Ludger , Haist Michael , Oneschkow Nadja
Published: January 2024

The expected long-term deformations of concrete structures are calculated using creep models, derived from experiments performed with constant mechanical loads. However, in the majority of real structures, such as bridges, constant creep loads are superimposed with cyclic loads of substantial magnitude. Additionally, such structures are subject to changes in environmental conditions (temperature and humidity). Deformation measurements of existing bridges, have shown significant underestimations by established creep models, which might be traced back to the superimposition of cyclic loads and different moisture contents. Therefore, the developments of strains, viscoplastic strains and modulus of elasticity under creep and cyclic loading of a normal strength concrete have been comparatively investigated for two different pore moisture contents (approx. 100% and 75%). The results show that viscous strains due to cyclic loading are significantly higher than those due to creep loading at the mean stress level of cyclic loading. Furthermore, the strains are higher for the higher moisture content. The differences in the development of the modulus of elasticity and viscoplastic strains of both load types give clear indication for load type dependent microstructural deformation mechanisms. The results obtained concerning the influence of the load type and the moisture content need to be considered for the improvement of existing models.
The effects of Phoniatric PREhabilitation in Head and Neck Cancer patients on Aspi-ration and Preservation of Swallowing (PREHAPS): Study protocol of a monocentric prospective randomized interventional outcome-blinded trial.
Authors: Dürr Stephan , Kuenzel Julian , Vester Sarah , Zeman Florian , Huppertz Gunnar , Koller Michael , Pfleger Gerda , Woertgen Annika , Salloum Hazem , Klinkhammer-Schalke Monika , Pukrop Tobias , Kummer Peter
Published: January 2024

Background:
Dysphagia, with its negative impact on life expectancy and quality of life, is a major side effect of head and neck squamous cell carcinoma (HNSCC). In a typical Head and Neck Cancer Center, more than half of patients are affected. Improving treatment, and ideally prevention respectively prehabilitation, therefore seems more than desirable.
Methods:
The study is planned as a monocentric, prospective, outcome-blinded, randomized interventional study comparing an advanced phoniatric-logopedic prehabilitation with a control (standard of care). Sixty patients (control group 30/intervention group 30) with an initial diagnosis of invasive HNSCC and curative treatment intention will be included over a period of 17 months. In addition to the previous standard, both groups will undergo both, detailed subjective assessment of swallowing function and quality of life by means of various questionnaires, as well as objective analyses by bioelectrical impedance measurements and phoniatric endoscopic swallowing examinations. In the intervention group, risk-related nutritional counseling (face-to-face) and phoniatric-logopedic prehabilitation are provided: Detailed counseling with video demonstration and exercises to strengthen and improve range of motion of the oral, pharyngeal, and laryngeal muscles (guided by exercise diary). Controls are performed 6 weeks, 3 and 6 months, as well as 9 or 12 months after the end of therapy during the regular tumor follow-up. Primary study endpoints are swallowing function and emotional distress at 6 weeks control visit.
Discussion:
Prehabilitation measures have already proven successful in other patient groups, e.g. transplant patients. In the field of head and neck oncology, interest in such concepts has increased significantly in recent years. However, usually only subgroups, e.g. patients with swallowing problems after radiochemotherapy alone, are in focus. Our study aims to investigate the general benefit of prehabilitation with regard to swallowing function, which is so important for protection of aspiration and quality of life.
Trial registration:
DRKS00029676 (German Clinical Trials Register https://drks.de/search/de/trial/DRKS00029676; International Clinical Trials Registry Platform https://trialsearch.who.int/Trial2.aspx?TrialID=DRKS00029676).
Preparation of Hybrid Fe 3 O 4 @SiO 2 Biopolymer Core-Shell Anticancer Drug Carrier for Sustainable Release of Doxorubicin
Authors: Chalal Samia , Haddadine Nabila , Thoburn John , Bakel Taha Yakoub , Dria Zakia , Bouslah Naima , Benaboura Ahmed , Beyaz Khaled , Guedouar Bendiba , Barille Regis , El-Shall Samy
Published: January 2024

Magnetic core-shell drug-carrier microcapsules were prepared and investigated using doxorubicin (Dox) as an anticancer drug model. First, superparamagnetic iron oxide nanoparticles (Fe3O4), were prepared and coated with silica SiO2 using a sol-gel method. Next, the Fe3O4@SiO2 nanoparticles were functionalized using the (3-aminopropyl) ethoxy silane (APS), to obtain the Fe3O4@SiO2-NH2. Afterward, the (Dox) was added to form the Fe3O4@SiO2-NH2-(Dox), and then the nanoparticles were wrapped in a polymer matrix by adding a chitosan/CaCl2 aqueous solution. The presence of divalent Ca2+ cations ensured hydrogel chain reticulation and the Fe3O4@SiO2-NH2-(Dox) encapsulation into the polymer matrix. The chitosan was chosen to shrink the hydrogel microcapsules' pores and limit the drug diffusion in acidic pH. Finally, a solution of dextran and polyvinyl pyrrolidone in chloroform was added to form the outermost shell structure. This core-shell magnetic drug-carrier microcapsule was noted system (A). The second drug-carrier microcapsule noted (B), was obtained by adding the (Dox) to the outermost polymer shell. The two-hybrid core-shell, drug carriers A, and B were observed by scanning electron microscopy (SEM) and characterized by ATR-FTIR spectroscopy, and their magnetic characteristics were confirmed. The drug diffusion kinetic study revealed that (A) had a greater drug release efficiency than (B), with an enhanced drug release effect at pH 5.8 and 7.4 associated with cancer cell environments, rather than pH 4.9 associated with healthy cell environments. Therefore, the magnetic drug carrier (A) was selected as a promising candidate for cancer cell targeting therapy.
The place of health in the EU-CELAC interregional cooperation from 2005 to 2023: a historical, empirical and prospective analysis.
Authors: Salgado Carolina
Published: January 2024

Background: much has been said by actors from different fields and perspectives about the manifold changes in world affairs triggered by the COVID-19 pandemic. In this context, it is to be expected that there will be impacts on long-standing partnerships such as the one between the European Union and the Community of Latin American and Caribbean Countries. However, few studies have demonstrated these impacts, either empirically, by uncovering their specificities or from a historical perspective, to allow for a reasonable methodological comparison of the patterns used to define the partnership and that have changed or have been affected in some way by the pandemic.
Results: through an in-depth qualitative assessment of primary and secondary sources, this article contributes to this research gap. It analyzes the patterns and changes or impacts in light of two strands of behavior that can make sense of EU-CELAC health cooperation - revisionist or reformist. The findings show an economy-driven health agenda as a new pattern of cooperation, which derives from EU reformist behavior after the pandemic.
Conclusions: the EU power to enforce its priorities in the context of health cooperation with CELAC is the main factor that will define how (and not just which) competing interests and capacities will be accommodated. The relevance of the study to the fields of global governance for health, interregional health cooperation and EU foreign policy is threefold. It shows us i.how two more international regimes are easily intertwined with health - trade and intellectual property - with the potential to deepen asymmetries and divergences even between long-standing strategic partners; ii.contrary to the idea that reformist behaviors are only adopted by actors who are dissatisfied with the status quo, the study shows us that the reformist actor can also be the one who has more material power and influence and who nevertheless challenges the success of cooperation in the name of new priorities and the means to achieve them; and iii.how the EU will find it difficult to operationalize its new priorities internally, among states and private actors, and with those of CELAC, given the history of intense disputes over health-related economic aspects.
Mechanical Methods for Materials Concentration of Lithium Iron Phosphate (LFP) Cells and Product Potential Evaluation for Recycling
Authors: Camargo Priscila Silva Silveira , Torres Gabriel Gomes Osório , Pacheco João Antônio Scherer , Cenci Marcelo Pilotto , Kasper Angela Cristina , Veit Hugo Marcelo
Published: January 2024

The production and sales of lithium-ion batteries (LIB) are very rapidly expanding nowadays, causing a significant impact on the consumption of critical raw materials, such as lithium. Thus, developing and improving methods for the separation and recovery of materials from lithium-ion batteries (LIB) is necessary to ensure the supply of critical raw materials, as well as to meet the recycling targets set by some countries. This study evaluated and compared two mechanical routes to concentrate materials of LiFePO4 (LPF) cells. In addition, the economic, environmental and scarcity risk potential of the products obtained through the best mechanical route were evaluated. The first route involved 6 grinding cycles in a knife mill, followed by particle size separation into 3 fractions. The second route involved a single grinding cycle (knife and hammer mill were tested), followed by particle size separation into 6 fractions. The second route showed more promise, with obtaining fractions rich in (1) iron, (2) aluminum and copper, and (3) cathode materials. Additionally, less operating time and energy consumption was necessary. The hammer mill offered a better separation for the iron and the cathodic materials (LiFePO4), while the knife mill showed to be more effective in concentrating the aluminum and copper. The product potential evaluation of the best route revealed that the priority fractions for recycling in economic and in the environmental assessment in LFP2 are 2 < n < 9.5 mm (due Cu and Al) and n < 0.5 mm (due Li). Considering the scarcity risk, priority should be assigned to the recycling of the fraction n < 0.5 due to lithium.
Oxidative stress induces ferroptosis in tendon stem cells by regulating mitophagy through cGAS-STING pathway during tendinopathy
Authors: Zheng shizhong , gao yuanyuan , Sun wenshuang , Wang junrui , Zhao danli , Tian haoyuan , Qiu yangling , Ji shufan , Wang shuqi , Fu qiuyu , zhang feng , Zhang zili , Wang feixia , Shao jiangjuan , Meng jia
Published: January 2024

Tendinopathy is one of the most prevalent sports injury diseases in orthopedics. However, there is no effective treatment or medicine. Recently, the discovery of tendon stem cells (TSCs) provides a new perspective to find new therapeutic methods for Tendinopathy. Studies have shown that oxidative stress will inevitably cause TSCs injury during tendinopathy, but the mechanism has not been fully elucidated. Here, we report the oxidative damage of TSCs induced by H2O2 via ferroptosis, as well, treatment with H2O2 raised the proportion of mitochondria engulfed by autophagosomes in TSCs. The suppression of mitophagy by Mdivi-1 significantly attenuates the H2O2-induced ferroptosis in TSCs. Mechanically, H2O2 actives the cGAS-STING pathway, which can regulate the level of mitophagy. Interfering with cGAS could impair mitophagy and the classical ferroptotic events. In the rat model of tendinopathy, interference of cGAS could relieve tendon injury by inhibiting ferroptosis. Overall, these results provided novel implications to reveal the molecular mechanism of tendinopathy, by which pointed to cGAS as a potential therapeutic target for the treatment of tendinopathy.
PLCβ1 By-Passes Early Growth Response -1 to Induce and Maintain the Differentiation of Neuronal Cells
Authors: Scarlata Suzanne , González-Burguera Imanol , Lin Guanyu , de Jesús Maider López , Saumell-Esnaola Miquel , Barrondo Sergio , Caño Gontzal García del , Sallés Joan
Published: January 2024

The Gαq/phospholipase C-β (PLCβ) signaling system mediates calcium responses to variety of hormones and neurotransmitters. Recent studies suggest that PLCβ1 expression plays a role in the differentiation of two types of cultured neuronal cells (PC12 and SKNSH) through a mechanism independent of Gαq. Here, we show a similar increase in PLCβ1 expression when human NT2 cells are induced to differentiate either through AraC or retinoic acid. Preventing this increase abolishes differentiation. Surprisingly, transfecting PLCβ1 into undifferentiated PC12 or NT2 cells induces differentiation without the need for differentiating agents. Studies to uncover the underlying mechanism focused on the transcription factor early growth response 1 (Egr-1) which mediates the expression of many proteins involved in differentiation including PLCb1. We find that increased levels of cytosolic PLCβ1 in undifferentiated PC12 and NT2 cells promote localization of Egr-1 to the nucleus. This shift to the nucleus is correlated disruption between Egr-1 and its cytosolic binding partner (Tar RNA binding protein) when PLCβ1 is over-expressed. These studies present a novel mechanism through which PLCβ1 can modulate differentiation.
Flutter instability characteristics and mechanisms of Ziegler double pendulum with arbitrary mass, stiffness and damping
Authors: Wang Xiaochen , Fan Chenguang , Yang Shuo , Yang Zhichun , Yang Yiren , Sinapius Michael
Published: January 2024

In this paper, the flutter instability characteristics and mechanisms of the weakly damped Ziegler double pendulum subjected to the follower type circular loading are investigated. The double pendulum is assumed to be with arbitrary masse, stiffness and damping. Different with the existed mathematical analysis methods, the pendulum flutter instability conditions and the corresponding critical parameters are theoretically deduced and evaluated based on an energy method with clear physical meanings. By introducing the ratio parameters of the structural mass, stiffness and damping coefficients, the complex influences of the structural mass, stiffness and damping are discussed in details. In addition to the counter-intuitive “damping Ziegler Paradox” influence, the stabilizing and destabilizing influences of the pendulum’s mass and stiffness are observed and investigated. To clarify these complex influences, the power flow characteristics on the pendulum flutter instability occurrence are investigated. The results indicate that the mass or stiffness related powers on each coordinate constitute the two “power exchange twins”, and can affect the related destabilizing or stabilizing influences of the pendulum mass and stiffness, while the “damping Ziegler Paradox” influence can be affected by the energy transmission efficiency between the stiffness related powers on each coordinate. Correspondingly the flutter instability physical mechanisms are clarified for the first time from the energy perspectives.
The Effect of Advanced Age on Peri- and Post-operative Complications Following Pelvic Floor Repair Surgeries
Authors: Shelef Goni , Rotchild Matan , Sade Shanny , Shoham Ilana Vardi , Weintraub Adi Y.
Published: January 2024

Objectives
To assess whether advanced age is a risk factor for complications following pelvic organ prolapse (POP) repair surgeries using the Clavien-Dindo classification system.
Methods
In this retrospective cohort study, 260 women who had undergone POP repair surgery at the Soroka University Medical Center (SUMC) between the years 2017–2019 were included. A univariate analysis was conducted to compare the demographical, clinical, obstetrical and operative characteristics of patients by age group (younger or older than 70 years). We performed a similar analysis to assess for the possible association between several variables and post-operative complications. Variables that were found to be associated with post-operative complications (P < 0.2) were included in a multivariate analysis along with advanced age.
Results
During the 12 months follow-up period, more than half of the women had experienced at least one post-operative complication. Minor complications (grades 1–2 according to the Clavian-Dindo classification system) were the most common. One woman had died during the follow-up period, and none had experienced organ failure (grade 4). Hysterectomy, as part of POP surgery, was found to be significantly associated with post-operative complications. Additionally, grandmultiparity (> 5 births) showed a tendency towards an increased risk for post-operative complications, however this reached only borderline significance. We found no association between advanced age and post-operative complications.
Conclusions
POP repair surgeries are safe for women of all ages. Major complications (grades 3–5) are rare in all age groups. Although advanced age was associated with a higher prevalence of comorbidity and a higher grade of prolapse, no significant difference in the post-operative complications was found between age groups. Concomitant hysterectomy at the time of POP repair surgery is a risk factor for post-operative complications.
Effects of external magnetic field on flow separation, control and reattachment
Authors: Shenoy Vinayak Vinod , S AJITH KUMAR
Published: January 2024

The paper attempts to analyze the effect of transverse magnetic field on flow over a backward-facing inclined step using the open-source CFD solver OpenFOAM. The equations governing the twodimensional, incompressible, laminar, electrically conducting flow are discretized and solved using the PISO algorithm. The simulations are conducted for various backward step angles (10◦ ≤ α ≤90◦) and Hartmann Numbers (0≤ M ≤30) at fixed kinematic (Re) and magnetic (Rem) Reynolds Numbers of 100 and 0.04 respectively. The influence of step angle and Hartmann number on the reattachment length is explored with the help of streamlines, streamwise pressure gradient, and velocity profiles.
Spatiotemporal transcriptome analysis reveals that conversion of IKFZ1-mediated microglia underlies a therapy for intracerebral hemorrhage
Authors: qiu wenqiao , mei jie , tian jie , Gao Mou , guo Lili , xu tao , liu wei , Zhu Jianwei , zhang yi , hou xiaolin , xie yao , Xiong Huan , li xinda , wang yangyang , gao mingjun , wu anguo , chen longyi , Huang Lulin
Published: January 2024

Intracerebral hemorrhage (ICH) is a type of stroke with high morbidity and mortality. Activation of microglia in response to ICH can lead to neuroinflammation, which can further exacerbate brain damage and neuronal death. By integration of single-cell RNA sequencing (scRNA-seq) and spatial transcriptome (ST) technologies in rat ICH model, we demonstrated that a microglia subtype (MIkzf1) with strong expression of Ikzf1 exhibits a stage-specific response to ICH around the damaged region. MIkzf1 expressed neuroinflammatory factors and markedly drove neuronal damage by accelerated pyroptosis. By downregulation of Ikzf1, MIkzf1 can be converted to promoting neuronal remodeling microglia subtype MPtn (high expression of Ptn), a neurite growth-promoting subtype, accompanied by the improved ICH outcome. Thus, Ikzf1 is an important promoter of pathogenic microglia MIkzf1 in ICH and suppressing Ikzf1 can reverse MIkzf1 to the repair subtype MPtn. These findings underscore the potential role of Ikzf1 as a new therapeutic target for ICH.
Demonstration of molecular chirality discrimination by resonance Raman scattering
Authors: Eginligil Mustafa , Zhao Shengkai , Baltabayeva Balzhan , Zhang Mingrui , Wang Jianmin , Hao Yufeng , Huang Wei
Published: January 2024

The pseudo-Jahn-Teller (PJT) effect, a direct indicator of molecular instability, can be the reason of sub-meV split degenerate electronic states that can be used to discriminate enantiomers. In the exemplar chiral molecule of camphor (C10H16O), the nondegenerate vibrational states were previously identified, but there has been no evidence of PJT states. Here, linearly polarized Raman measurements were employed to demonstrate the PJT states, with splitting of 1.8 ± 0.2 cm-1, for scattering by chiral centers on carbon ring of camphor. This observation is attributed to the symmetry reduction and chirality-dependent C3 symmetry becoming intact by placing camphor on a SiO2/Si substrate. The depolarization ratio, the noncoincidence effect, and the molecular chirality-dependent polarization rotation of the PJT states manifested a clear distinction between enantiomers. More importantly, quantification of chirality was possible based on the occupancy of the PJT states at the depolarized state, which is applicable to similar chiral molecules.
TGEV non-structural protein ORF3b up-regulates the expression of SLA-DR at transcriptional level in monocyte-derived porcine dendritic cells
Authors: Ma Mengyao , Jia Xinhao , Liu hang , Qian Mengwei , Pang Bo , Zhang Honglei , Ma Shijie , zheng lanlan
Published: January 2024

Transmissible gastroenteritis virus (TGEV) is a porcine intestinal pathogenic coronavirus that can cause acute intestinal diseases in pigs, especially in suckling pigletsunder two weeks with the mortality rate of 100%. Dendritic cell (DC) is an important antigen-presenting cell (APC), which plays vital roles in the initiation and regulation of immune responses in animals. In this study, we have used monocyte-derived porcine DCs as the in vitro model of APC to further study the pathogenic mechanism of TGEV. Our results revealed that TGEV could replicate in the monocyte-derived porcine DCs, while UV-inactivated TGEV failed to infect the cells. Notably, we found that TGEV infection of DCs resulted in a significant increase of the expression of swine leukocyte antigen II DR (SLA-DR), a vital molecule of the major histocompatibility complex class II (MHC-II) family. And we further demonstrated that TGEV non-structural protein ORF3b significantly promoted the expression of SLA-DR at the transcriptional level in porcine DCs. The results of this study help us better clarify the pathogenic mechanism of TGEV.
Test-Retest Repeatability of Patlak Slopes versus Standardized Uptake Values for Hypermetabolic Lesions and Normal Organs in an Oncologic PET/CT Population
Authors: Ince Semra , Laforest Richard , Ashrafinia Saeed , Smith Anne M. , Fraum Tyler J. , Wahl Richard L.
Published: January 2024

Purpose: We aimed to determine the test-retest repeatability of quantitative metrics based on the Patlak slope (PS) versus the standardized uptake value (SUV) among lesions and normal organs on oncologic [18F]FDG-PET/CT.
Procedures: This prospective, single-center study enrolled adults undergoing standard-of-care oncologic [18F]FDG-PET/CTs. Early (35-50 min post-injection) and late (75-90 min post-injection) SUV and PS images were reconstructed from dynamic whole-body PET data. Repeat imaging occurred within 7 days. Relevant quantitative metrics were extracted from lesions and normal organs. Repeatability was assessed via mean test-retest percent changes [T-RT %Δ], within-subject coefficients of variation (wCVs), and intra-class correlation coefficients (ICCs).
Results: Nine subjects (mean age, 61.7 ± 6.2 years; 6 females) completed the test-retest protocol. Four subjects collectively had 17 [18F]FDG-avid lesions. Lesion wCVs were higher (i.e., worse repeatability) for PS-early-max (16.2%) and PS-early-peak (15.6%) than for SUV-early-max (8.9%) and SUV-early-peak (8.1%), with similar early metric ICCs (0.95-0.98). Lesion wCVs were similar for PS-late-max (8.5%) and PS-late-peak (6.4%) relative to SUV-late-max (9.7%) and SUV-late-peak (7.2%), with similar late metric ICCs (0.93-0.98). There was a significant bias toward higher retest SUV and PS values in the lesion analysis (T-RT %Δ [95% CI]: SUV-late-max, 10.0% [2.6%, 17.0%]; PS-late-max, 20.4% [14.3%, 26.4%]) but not in the normal organ analysis.
Conclusion: Among [18F]FDG-avid lesions, the repeatability of PS-based metrics is similar to equivalent SUV-based metrics at late post-injection time points, making PS-based metrics suitable for tracking response to oncologic therapies. However, evaluation was somewhat limited by a bias toward higher retest values.
Diversity of Desmids in the Thattekkad Bird Sanctuary, Kerala, India: A Comprehensive Exploration
Authors: Bibina Puthuvalnikarthil Babu , Praseetha Ambazhathodi Prabhakaran , Ammini Chenathuparmbil Jose , Sreeku Vadakkethil Balakrishnan , Paul Parapully Tessy
Published: January 2024

Desmids are a group of exclusively freshwater green algae known for their remarkably diverse and captivating forms. Desmids are valued organisms due to their exceptional sensitivity to environmental changes, so they are considered to be potential tools for effective aquatic conservation management. The present study tries to account for the diversity of desmids in Thattekkad Bird Sanctuary, situated in Ernakulam District, the first Bird Sanctuary in Kerala and lies within the geographical limits of 10°08′ N 76°41′ E and 10.13° N 76.68° E. The study was carried out over a period of one year, from March 2022 to April 2023, and identified a total of seventy-four taxa, which are distributed under four families and 18 genera. Predominant genera included were Cosmarium (21), Euastrum (12), and Staurastrum (8). Notably Heimansia pusilla (L. Hilse) Coesel, Euastrum praemorsum (Nordstedt) Schmidle, Euastrum jenneri W. Archer, Staurastrum saltans Joshua var. javanicum Scott and Prescott, Cosmarium clepsydra Nordstedt var. bicardia (Reinsch) Croasdale are new reports to India, five species are new reports to Kerala and all species being novel to Thattekkad Bird sanctuary.
Characterization of Rice Accessions using Info-chemical and Visual Cues Emitted to Biologically Control Diopsis apicalis Dalman
Authors: Bocco Roland , Pegalepo Esther , Tamò Manuele , Togola Abou , Gandonou Christophe Bernard , Ndjiondjop Marie Noelle , Nwilene Francis , Sow Mounirou
Published: January 2024

This study assessed the host plant selection behavior of female stalk-eyed flies (SEF) or Diopsis apicalis, where a Y-tube olfactometer was used to compare the attractiveness of SEF to the odor of leaves from four rice varieties (ITA306, WAB56-104, CG14, and RAM55). Another step of the evaluation consisted of pairing leaf odors from two rice varieties. Also, potted plants of the tested varieties were displayed in a screened cage and submitted to SEF female selection. Results showed that the odor produced by leaves from rice varieties CG14, WAB56-104, and ITA306 significantly attracted SEF with 81%, 70%, and 97%, respectively, while SEF females were rarely attracted by the odor of leaves from the resistant rice variety RAM55 with 35%. Results suggested that the use of a Y-tube olfactometer was similar to that obtained in the case of a screened cage. Resistance exhibited by rice variety CG14 against SEF is related to an antibiosis mechanism, while that in RAM55 is an antixenosis one. The authors recommend CG14 and RAM55 as candidates for breeding to create resistant lines against SEF.
Ensemble-based design of tau to inhibit aggregation while preserving biological activity
Authors: Joachimiak Lukasz , Bali Sofia , Singh Ruhar , Wydorski Pawel , Wosztyl Aleksandra , Perez Valerie , Chen Dailu , Rizo Josep
Published: January 2024

The microtubule-associated protein tau is implicated in neurodegenerative diseases characterized by amyloid formation. Mutations associated with frontotemporal dementia increase tau aggregation propensity and disrupt its endogenous microtubule-binding activity. The structural relationship between aggregation propensity and biological activity remains unclear. We employed a multi-disciplinary approach, including computational modeling, NMR, cross-linking mass spectrometry, and cell models to design tau sequences that stabilize its structural ensemble. Our findings reveal that substitutions near the conserved ‘PGGG’ beta-turn motif can modulate local conformation, more stably engaging in interactions with the 306VQIVYK311 amyloid motif to decrease aggregation in vitro and in cells. Designed tau sequences maintain microtubule binding and explain why 3R isoforms of tau exhibit reduced pathogenesis over 4R isoforms. We propose a simple mechanism to reduce the formation of pathogenic species while preserving biological function, offering insights for therapeutic strategies aimed at reducing protein misfolding in neurodegenerative diseases.
Development of a parametric design and optimization platform for a helicopter tail-rotor driveline
Authors: Huang Zhonghe , Dong Jianxiong , Sang Yanwei , Liu Chuliang
Published: January 2024

A helicopter tail-rotor driveline incorporates multiple shafts, couplings, and dampers to transmit power from the middle gear to the tail propeller. Designers face a challenge in adjusting parameters for these components due to a lack of reference information on parameter design and optimization. To address this gap, this study presents a parametric design software platform for facilitating parameter analysis and optimization. A mathematical model is developed to capture the system behavior and precise boundary conditions, considering all relevant components and their interactions. The software design issues, basic modules, architecture, and interfaces are developed using MATLAB App Designer. Generative design is employed to create a damper's parameters optimization function, combining a design-driven process with analytical calculations and expandable multi-objective algorithms. A numerical calculation module and a response analysis module are designed to present comprehensive dynamic responses. The software is utilized to simulate a helicopter tail-rotor driveline, providing insights into multi-stage damping characteristics and the combined effects of rub impact and misalignment. A multi-objective optimization case is conducted by generative design module to enhance damping and durability of the damper. The simulation results of the software are validated using test results from a test rig designed to mimic an actual helicopter tail-rotor driveline. This study serves as a valuable reference for software development in shaft/damper systems and similar rotor machinery.
Prediction Model for Lateral Lymph Node Metastasis of Papillary Thyroid Carcinoma in Children and Adolescents Based on Ultrasound Imaging and Clinical Features: a retrospective study
Authors: Lin Shiyang , Zhong Yuan , Lin Yidi , Liu Guangjian
Published: January 2024

Background
Lateral lymph node metastases (LNM) for pediatric patients with papillary thyroid cancer (PTC) is an independent risk factor for recurrence. We aimed to identify risk factors and establish a prediction model for lateral LNM before surgery in children and adolescents with PTC.
Methods
A retrospective cohort study was conducted with data obtained from 63 minors with PTC between 2014 and 2023. The clinical factors, ultrasound (US) features of primary tumor, and pathology records of patients were collected and analyzed. Categorical variables were analyzed using the χ2 test. Logistic regression analysis was used to determine independent predictors. The prediction model is established from the regression coefficient. The area under receiver operating characteristic (ROC) curve was used to evaluate the predictive performance of risk factors and the combined predictor.
Results
21 minors with PTC had lateral LNM (33.3%). Logistic regression revealed that independent risk factors of lateral LNM were multifocality, tumor size, sex, and age. The prediction model is: 6.48–2.87 × Tumor size- 1.57 × Multifocality- 2.43 × Sex- 4.06 × Age. Compared to single risk factor, the area under the receiver operating characteristic curve (AUC) of the combined predictors was significantly higher (0.842) with a sensitivity of 71.4% and a specificity of 81.0%.
Conclusions
The independent risk factors for lateral LNM were multifocality and tumor size of primary tumor on US imaging, sex and age. Our model provides better performance than US imaging and clinical features along in predicting the status of lateral LNM for minors PTC patients.