bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Blade-Coating Conditions on the Electrical and Optical Properties of Transparent Ag Nanowire Electrodes
Authors: Yoon Hyungsub , Matteini Paolo , Hwang Byungil
Published: January 2023

Optimizing the coating conditions for a doctor blading system is important when seeking to improve the performance of Ag nanowire electrodes. In this study, the effect of the blading height and speed on the optical and electrical properties of Ag nanowire electrodes was investigated. Ag nanowires were first spread on a PET substrate using a doctor blade with differing heights at a fixed blading speed. An increase in the blading height resulted in the degradation of the optical transmittance and stronger haze due to the higher probability of Ag nanowire agglomeration arising from the greater wet thickness. When the blading speed was varied, the optical transmittance and haze were unaffected up until 20 mm/s, followed by minor degradation of the optical properties at blading speeds over 25 mm/s. The higher speeds hindered the spread of the Ag nanowire solution, which also increased the probability of Ag nanowire agglomeration. However, this degradation was less serious compared to that observed with a change in the blading height. Therefore, optimizing the blading height was confirmed to be the priority for the production of high-performance transparent Ag nanowire electrodes. Our study thus provides practical guidance for the fabrication of Ag nanowire electrodes using doctor blading systems.
Functional Surface Generation by EDM—A Review
Authors: Nafi Muhammad Abdun , Jahan Muhammad Pervej
Published: January 2023

Electro-discharge machining (EDM) removes electrically conductive materials by high frequency spark discharges between the tool electrode and the workpiece in the presence of a dielectric liquid. Being an electrothermal process and with melting and evaporation being the mechanisms of material removal, EDM suffers from migration of materials between the tool and the workpiece. Although unwanted surface modification was considered a challenge in the past for many applications, this inherent nature of the EDM process has recently become of interest to the scientific community. As a result, researchers have been focusing on using the EDM process for surface modification and coating by targeted surface engineering. In order to engineer a surface or generate functional coatings using the electro-discharge process, proper knowledge of the EDM process and science of electro-discharge surface modification must be understood. This paper aims to provide an overview of the electro-discharge surface modification and coating processes, thus assisting the readers on exploring potential applications of EDM-based techniques of surface engineering and coating generation. This review starts with a brief introduction to the EDM process, the physics behind the EDM process, and the science of the surface modification process in EDM. The paper then discusses the reasons and purposes of surface modification and coating practices. The common EDM-based techniques reported in the literature for producing coatings on the surface are discussed with their process mechanisms, important parameters, and design considerations. The characterization techniques used for the analysis of modified surfaces and coating layers, as well as the tribological and surface properties of modified surfaces or coatings are discussed. Some of the important applications of EDM-based surface modification and coating processes are generating surfaces for protective coating, for aesthetic purposes, for enhancing the biocompatibility of implants, for improving corrosion resistance, for improving wear resistance, and for improving tribological performance. The current state of the research in these application areas is discussed with examples. Finally, suggestions are provided on future research directions and innovative potential new applications of the electro-discharge-based surface engineering and coating processes.
Exploring the Structural Variability of Dynamic Biological Complexes by Single-Particle Cryo-Electron Microscopy
Authors: DiIorio Megan , Kulczyk Arkadiusz
Published: January 2023

Biological macromolecules and assemblies precisely rearrange their atomic 3D structures to execute cellular functions. Understanding the mechanisms by which these molecular machines operate requires insight into the ensemble of structural states they occupy during the functional cycle. Single-particle cryo-electron microscopy (cryo-EM) has become the preferred method to provide near-atomic resolution, structural information about dynamic biological macromolecules elusive to other structure determination methods. Recent advances in cryo-EM methodology have allowed structural biologists not only to probe the structural intermediates of biochemical reactions, but also to resolve different compositional and conformational states present within the same dataset. This article reviews newly developed sample preparation and single-particle analysis (SPA) techniques for high-resolution structure determination of intrinsically dynamic and heterogeneous samples, shedding light upon the intricate mechanisms employed by molecular machines and helping to guide drug discovery efforts.
Interaction Energy Dependency on Pulse Width in ns NIR Laser Scanning of Silicon
Authors: Li Shunping , Wang Xinchang , Chen Guojie , Wang Zhongke
Published: January 2023

Laser ablation of semiconductor silicon has been extensively studied in the past few decades. In the ultrashort pulse domain, whether in the fs scale or ps scale, the pulse energy fluence threshold in the ablation of silicon is strongly dependent on the pulse width. However, in the ns pulse scale, the energy fluence threshold dependence on the pulse width is not well understood. This study elucidates the interaction energy dependency on pulse width in ns NIR laser ablation of silicon. The level of ablation or melting was determined by the pulse energy deposition rate, which was proportional to laser peak power. Shorter pulse widths with high peak power were likely to induce surface ablation, while longer pulse widths were likely to induce surface melting. The ablation threshold increased from 5.63 to 24.84 J/cm2 as the pulse width increased from 26 to 500 ns. The melting threshold increased from 3.33 to 5.76 J/cm2 as the pulse width increased from 26 to 200 ns, and then remained constant until 500 ns, the longest width investigated. Distinct from a shorter pulse width, a longer pulse width did not require a higher power level for inducing surface melting, as surface melting can be induced at a lower power with the longer heating time of a longer pulse width. The line width from surface melting was less than the focused spot size; the line appeared either as a continuous line at slow scanning speed or as isolated dots at high scanning speed. In contrast, the line width from ablation significantly exceeded the focused spot size.
Pathogenic Leptospira Infections in Hubei Province, Central China
Authors: Xu Jiale , Chen Jintao , Xiong Chaorui , Qin Lingxin , Hu Bing , Liu Manqing , Ren Yuting , Li Yirong , Cai Kun , Chen Liangjun , Hou Wei
Published: January 2023

Leptospirosis is an important zoonosis that is caused by pathogenic Leptospira, which is considered to be a re-emerging infectious disease in many countries. Rodents are the most important reservoirs for both human and animal infection. An epidemiological survey of pathogenic Leptospira in rodents is important for the prevention and control of leptospirosis. In this study, a total of 964 rodents were captured from six cities in Hubei Province, and two pathogenic Leptospira species (L. interrogans and L. borgpetersenii) were detected using nested PCR with an overall prevalence of 4.8%. L. interrogans was distributed in five sampling sites, which may be the dominant species of pathogenic Leptospira in Hubei Province. In addition, Rattus norvegicus showed a relatively high infection rate, which may play an important role in the transmission and infection of pathogenic Leptospira. This study reveals the prevalence of pathogenic Leptospira in wild rodents in Hubei Province, suggesting that the risk of leptospirosis infection in Hubei Province still exists.
Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs
Authors: Moravkova Monika , Kostovova Iveta , Kavanova Katerina , Pechar Radko , Stanek Stanislav , Brychta Ales , Zeman Michal , Kubasova Tereza
Published: January 2023

Restrictions on the use of antibiotics in pigs lead to the continuous search for new probiotics serving as an alternative to antibiotics. One of the key parameters for probiotic bacteria selection is the absence of horizontally transmissible resistance genes. The aim of our study was to determine antibiotic susceptibility profiles in 28 Lactobacillus amylovorus isolates derived from the digestive tract of wild boars and farm pigs by means of the broth microdilution method and whole genome sequencing (WGS). We revealed genetic resistance determinants and examined sequences flanking resistance genes in these strains. Our findings indicate that L. amylovorus strains from domestic pigs are predominantly resistant to tetracycline, erythromycin and ampicillin. WGS analysis of horizontally transmissible genes revealed only three genetic determinants (tetW, ermB and aadE) of which all tetW and ermB genes were present only in strains derived from domestic pigs. Sequence analysis of coding sequences (CDS) in the neighborhood of the tetW gene revealed the presence of site-specific recombinase (xerC/D), site-specific DNA recombinase (spoIVCA) or DNA-binding transcriptional regulator (xre), usually directly downstream of the tetW gene. In the case of ermB, CDS for omega transcriptional repressor or mobilization protein were detected upstream of the ermB gene.
Oral Prevalence of Akkermansia muciniphila Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients
Authors: Shen Ching , Clawson James Brigham , Simpson Justin , Kingsley Karl
Published: January 2023

Akkermansia muciniphila (AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of evidence, the primary goal of this project is to survey an existing saliva repository to determine the overall prevalence of this organism and any associations with demographic or patient characteristics (age, sex, body mass index, race/ethnicity, orthodontic therapy). Using an approved protocol,, a total n = 141 pediatric samples from an existing saliva repository were screened using qPCR revealing 29.8% harbored AM with nearly equal distribution among males and females, p = 0.8347. Significantly higher percentages of pediatric, non-orthodontic patients were positive for AM (42.3%) compared with age-matched orthodontic patients (14.3%)—which were equally distributed among non-orthodontic males (42.1%) and non-orthodontic females (42.5%). In addition, analysis of the adult samples revealed that nearly equal percentages of males (18.2%) and females (16.7%) harbored detectable levels of salivary AM, p = 0.2035. However, a higher proportion of non-orthodontic adult samples harbored AM (21.3%) compared to orthodontic samples (12.8%, p = 0.0001), which was equally distributed among males and females. These results suggest that both age and the presence of orthodontic brackets may influence microbial composition and, more specifically, are associated with reduction in AM among both pediatric and adult populations from their baseline levels.
Agates from Mesoproterozoic Volcanics (Pasha–Ladoga Basin, NW Russia): Characteristics and Proposed Origin
Authors: Svetova Evgeniya N. , Svetov Sergei A.
Published: January 2023

Agate gemstones occurring in the Mesoproterozoic volcanic rocks of the Priozersk Formation (PrF) within the Pasha–Ladoga Basin (Fennoscandian Shield, NW Russia) were investigated to characterize the mineral and geochemical composition of the agates and provide new information concerning their origin. Optical and scanning electron microscopy, EDS microanalysis, X-ray powder diffraction, X-ray fluorescence spectrometry, Raman spectroscopy, inductively coupled plasma mass spectrometry (ICP-MS), and C-O isotope analysis were used for the study. Agate mineralization appears mostly as an infill of fissures, cavities, gas vesicles in massive and vesicular basalts, lava-breccias. The mineral composition of agates is dominated by alpha-quartz (fibrous chalcedony, microcrystalline and macrocrystalline quartz), but it also displays abundances of calcite. The characteristic red-brownish agate’s coloration is caused by multiple hematite inclusions distributed in an agate matrix. The study revealed the two phases of agate formation in the PrF volcanics, which are most likely controlled by two distinctly different fluids and/or their mixture. At first, agates appeared due to post-magmatic iron-rich fluids. The late hydrothermal activity was probably triggered by intrusion of gabbro-dolerite sill and resulted in the second phase of agate formation. We suggest that the late hydrothermal fluids remobilized the iron compounds from the crust of weathering underlying the PrF volcanics, which led to additional formation of vein agates and filling of gas vesicles with hematite-rich calcite/silica matter.
Effect of High Temperature Reconstruction and Modification on Phase Composition and Structure of Steel Slag
Authors: Hao Shuai , Luo Guoping , Lu Yuanyuan , An Shengli , Chai Yifan , Song Wei
Published: January 2023

This study investigates the pattern of influence of blast furnace slag tempering on the composition and structure of steel slag. The chemical composition, equilibrium phase composition, microscopic morphological characteristics and elemental composition of microscopic regions of steel slag and blast furnace slag modified by high temperature reconstruction were analyzed using X-ray diffractometer (XRD), FactSage7.1 thermodynamic analysis software, mineral phase microscopy and field emission scanning electron microscopy. The results show that blast furnace slag blending can promote the generation of a low melting point phase in the slag, as well as reducing its melting temperature and improving its high temperature kinetic conditions. On the one hand, the incorporation of blast furnace slag was found to promote the generation of C2S in the steel slag and improve its gelling activity. Notably, at 1400 °C, the C2S content (mass fraction) of steel slag modified with 15% high temperature reconstruction reached 39.04%, while that of unmodified steel slag at this temperature was only 16.92%, i.e., only 1/4 of the C2S content in the modified slag. On the other hand, the incorporation of blast furnace slag inhibited the generation of a-C2S-C3P and calcium ferrate minerals, refined the grains of calcium–aluminum yellow feldspar, reduced the alkalinity and promoted the generation of silicate phases with high hydration activity in steel slag.
Selective Adsorption of Sodium Silicate on the Surface of Bastnaesite and Fluorite in Salicylhydroxamic Acid System under Alkaline Conditions
Authors: Wang Maoyuan , Xiong Wenliang , Xiao Junhui , Guo Yao , Deng Jie , Chen Da , Ouyang Anni , Lei Menglin , Zhang Lijun
Published: January 2023

During the flotation separation process of bastnaesite, it is difficult to separate bastnaesite from fluorite effectively. In this present study, sodium silicate (SS) can effectively improve the flotation separation effect of bastnaesite and fluorite in salicylhydroxamic acid (SHA) systemasa. Through relevant analyses, such as Zeta potential measurements, adsorption capacity tests, Fourier transform infrared (FTIR) spectroscopic analyses and X-ray photoelectron spectroscopy (XPS) tests, the selective suppressor of SS on fluorite was proven. At pH 10, the single mineral flotation results show that with the increase of SS dosage, the flotation recovery of fluorite rapidly decreases from 61.5% to 35.31%, while the flotation rate of bastnaesite is still high (recovery is 80.02%). Then, the experiment of artificial mixed ore proved that the flotation separation of fluorite and bastnaesite was effective under the appropriate dosage of inhibitor. The results of potentiodynamic measurement and an adsorption capacity test showed that the SiOOH3− structure of SS more easily reacted with fluorite, which further prevented the adsorption of SHA on the fluorite surface. FTIR test results and XPS analysis further showed that SS had a strong binding effect with the Ca site on the fluorite surface, but a weak binding effect with the Ce site on the bastnaesite surface. Consequently, SS can be used as an effective inhibitor in the flotation separation of fluorite and bastnaesite.
Synthesis and Antiprotozoal Activity of Azabicyclo-Nonane Pyrimidine Hybrids
Authors: Hinteregger Clemens , Dolensky Johanna , Seebacher Werner , Saf Robert , Mäser Pascal , Kaiser Marcel , Weis Robert
Published: January 2023

2,4-Diaminopyrimidines and (dialkylamino)azabicyclo-nonanes possess activity against protozoan parasites. A series of fused hybrids were synthesized and tested in vitro against pathogens of malaria tropica and sleeping sickness. The activities and selectivities of compounds strongly depended on the substitution pattern of both ring systems as well as on the position of the nitrogen atom in the bicycles. The most promising hybrids of 3-azabicyclo-nonane with 2-aminopyrimidine showed activity against P. falciparum NF54 in submicromolar concentration and high selectivity. A hybrid with pyrrolidino substitution of the 2-azabicyclo-nonane as well as of the pyrimidine moiety exhibited promising activity against the multiresistant K1 strain of P. falciparum. A couple of hybrids of 2-azabicyclo-nonanes with 2-(dialkylamino)pyrimidines possessed high activity against Trypanosoma brucei rhodesiense STIB900 and good selectivity.
Advances in the Synthesis of Fused 1,2,3-Triazoles via a MCR-Intramolecular Azide-Alkyne Cycloaddition Approach
Authors: P Rahul , Thomas Joice , Dehaen Wim , John Jubi
Published: January 2023

The present review narrates several reports which deal with the synthesis of fused 1,2,3-triazole containing scaffolds following a sequential multicomponent reaction (MCR)—intramolecular azide-alkyne cycloaddition (IAAC) approach. The reviewed reactions were cleverly designed so as to incorporate azide and alkyne functionalities in the MCR product which was then subjected to IAAC. The review is divided into two sections based on the number of components in the multicomponent reaction. We have aimed at a critical discussion and also have highlighted either advantages or disadvantages of each methodology.
Effects of Sonication and Thermal Pasteurization on the Nutritional, Antioxidant, and Microbial Properties of Noni Juice
Authors: Choo Yi Xuan , Teh Lai Kuan , Tan Chin Xuan
Published: January 2023

Sonication is recognized as a potential food processing method to improve the functional properties of fruit juice. This study evaluated the effects of different sonication durations (20, 40, and 60 min) and thermal pasteurization on the nutritional, antioxidant, and microbial properties of noni juice. Fresh noni juice served as the control. The main organic acids detected were malic (57.54–89.31 mg/100 mL) and ascorbic (17.15–31.55 mg/100 mL) acids. Compared with the fresh sample, the concentrations of these compounds were significantly improved (p < 0.05) in the 60 min sonicated sample but reduced (p < 0.05) in the pasteurized sample. Moreover, sonication for 60 min resulted in increments of scopoletin, rutin, and vanillic acid compared to the fresh sample. The antioxidant activity of the juice sample was improved in the sample sonicated for 60 min. Irrespective of juice processing method, the level of microbial counts in noni juice was within the satisfactory level over the 8 weeks of refrigerated (4 °C) storage. This study highlights the feasibility of using ultrasound processing to enhance the quality of noni juice on the industrial scale.
Efficient Hydrogen and Oxygen Evolution Catalysis Using 3D-Structured Nickel Phosphosulfide Nanosheets in Alkaline Media
Authors: Lin Lei , Fu Qiang , Hu Junbei , Wang Ran , Wang Xianjie
Published: January 2023

Water electrolysis offers a zero-carbon route to generate renewable energy conversion systems. Herein, a self-supported nickel phosphosulfide nanosheet (NS) electrocatalyst was fabricated at a low temperature on carbon cloth, which was then subjected to Ar etching to enhance its catalytic activity. Etching resulted in better hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) performance than other samples, with overpotentials of 103.1 mV (at 10 mA cm−2) and 278.9 mV (at 50 mA cm−2), respectively. The characterization results confirmed that Ar etching created a thin amorphous layer around the NiPS3 NSs, which increased the number of active sites and modulated their electronic structures. These 3D-structured NiPS3 NSs and their subsequent Ar etching process show promise for applications in overall water splitting in alkaline media.
Investigations on Potential Applications of CaMg(CO3)2 Nanoparticles
Authors: Han Ihn , Rhee Chulwoo , Kim Doyoung
Published: January 2023

Calcium magnesium carbonate nanoparticles (CaMg(CO3)2 NPs), well-known as dolomite, are formed by the replacement of half of the calcite minerals of limestone. The dolomite (CaMg(CO3)2) nanoparticles are composed of calcite (CaCO3) and magnesium carbonate (MgCO3), both of which offer promising strategies for maintaining growth and development in mammals and agricultural plants. A grounded mixture of dolomite limestone was prepared via colloidal precipitates for the synthesis of CaMg(CO3)2 NPs, and their characteristics were examined using XRD, particle size analysis by DLS, and surface morphology by SEM and TEM. X-ray photoelectron spectroscopy was used to investigate the binding energy of each element of the dolomite NPs. Spectroscopy revealed that colloidal precipitation is the ideal method for producing NPs. We assessed the numerous beneficial impacts of CaMg(CO3)2 NPs in diverse sectors such as agriculture, cancer treatment, and microbiology in this study. Furthermore, an in vivo study was also carried out on chickens to observe the effects of CaMg(CO3)2 NPs. The obtained results showed that the treated group with CaMg(CO3)2 NPs maintained a more uniform calcium absorption rate than the control group did. The findings of this study suggest that CaMg(CO3)2 NPs operate as a stimulant for plants and as an inhibitory agent for bacteria and cancer cells.
Repurposed Drugs in Gastric Cancer
Authors: Araújo Diana , Ribeiro Eduarda , Amorim Irina , Vale Nuno
Published: January 2023

Gastric cancer (GC) is one of the major causes of death worldwide, ranking as the fifth most incident cancer in 2020 and the fourth leading cause of cancer mortality. The majority of GC patients are in an advanced stage at the time of diagnosis, presenting a poor prognosis and outcome. Current GC treatment approaches involve endoscopic detection, gastrectomy and chemotherapy or chemoradiotherapy in an adjuvant or neoadjuvant setting. Drug development approaches demand extreme effort to identify molecular mechanisms of action of new drug candidates. Drug repurposing is based on the research of new therapeutic indications of drugs approved for other pathologies. In this review, we explore GC and the different drugs repurposed for this disease.
New Synthetic Quinoline (Qui) Derivatives as Novel Antioxidants and Potential HSA’s Antioxidant Activity Modulators—Spectroscopic Studies
Authors: Rogóż Wojciech , Owczarzy Aleksandra , Kulig Karolina , Zięba Andrzej , Maciążek-Jurczyk Małgorzata
Published: January 2023

The antioxidant activity of drugs, as well as the influence of drugs on the activity of endogenous antioxidant mechanisms in the human body is of great importance for the course of the disease and the treatment process. Due to the need to search for new therapeutic methods, the study of newly synthesized substances with potential therapeutic activity is necessary. This study aimed to designate some properties and characteristic parameters of new, synthetic quinoline three derivatives—1-methyl-3-allylthio-4-(4′-methylphenylamino)quinolinium bromide (Qui1), 1-methyl-3-allylthio-4-(3′-hydroxyphenylamino)quinolinium bromide (Qui2) as well as 1-methyl-3-allylthio-4-(4′-hydroxyphenylamino)quinolinium bromide (Qui3), including their antioxidant properties, as well as to analyse their activity as the potential modulators of Human Serum Albumin (HSA) antioxidant activity. In order to achieve the goal of the study, spectroscopic methods such as UV-Vis and circular dichroism (CD) spectroscopy have been used and based on the obtained data only slight and probably some surface interaction of quinoline derivatives (Qui1–Qui3) with HSA have been observed. The effect of Qui1–Qui3 on the HSA secondary structure was also insignificant. All analysed quinine derivatives have antioxidant activity against ABTS cation radical, in turn against DPPH radical, only Qui3 has noticeable antioxidant potential. The highest reduction potential by Qui3 as well as (Qui3 + HSA)complex has been shown. Qui3 mixed with HSA has mostly the synergistic effect against DPPH, ABTS and FRAP, while Qui1 and Qui2 in the presence of HSA mostly have a synergistic and additive effect towards ABTS, respectively. Based on the obtained results it can be concluded that Qui2 and Qui3 can be considered potential modulators of HSA antioxidant activity.
Recent Advances in Molecularly Imprinted Polymers for Antibiotic Analysis
Authors: Zhao Guangli , Zhang Yue , Sun Dani , Yan Shili , Wen Yuhao , Wang Yixiao , Li Guisheng , Liu Huitao , Li Jinhua , Song Zhihua
Published: January 2023

The abuse and residues of antibiotics have a great impact on the environment and organisms, and their determination has become very important. Due to their low contents, varieties and complex matrices, effective recognition, separation and enrichment are usually required prior to determination. Molecularly imprinted polymers (MIPs), a kind of highly selective polymer prepared via molecular imprinting technology (MIT), are used widely in the analytical detection of antibiotics, as adsorbents of solid-phase extraction (SPE) and as recognition elements of sensors. Herein, recent advances in MIPs for antibiotic residue analysis are reviewed. Firstly, several new preparation techniques of MIPs for detecting antibiotics are briefly introduced, including surface imprinting, nanoimprinting, living/controlled radical polymerization, and multi-template imprinting, multi-functional monomer imprinting and dummy template imprinting. Secondly, several SPE modes based on MIPs are summarized, namely packed SPE, magnetic SPE, dispersive SPE, matrix solid-phase dispersive extraction, solid-phase microextraction, stir-bar sorptive extraction and pipette-tip SPE. Thirdly, the basic principles of MIP-based sensors and three sensing modes, including electrochemical sensing, optical sensing and mass sensing, are also outlined. Fourthly, the research progress on molecularly imprinted SPEs (MISPEs) and MIP-based electrochemical/optical/mass sensors for the detection of various antibiotic residues in environmental and food samples since 2018 are comprehensively reviewed, including sulfonamides, quinolones, β-lactams and so on. Finally, the preparation and application prospects of MIPs for detecting antibiotics are outlined.
Efficient Adsorption and Extraction of Glutathione S-Transferases with Glutathione-Functionalized Graphene Oxide–Polyhedral Oligomeric Silsesquioxane Composite
Authors: Sun Jingqi , Jia Limin , Chen Xuwei
Published: January 2023

Glutathione S-transferases (GSTs) are important type-II detoxification enzymes that protect DNA and proteins from damage and are often used as protein tags for the expression of fusion proteins. In the present work, octa-aminopropyl caged polyhedral oligomeric silsesquioxane (OA–POSS) was prepared via acid-catalyzed hydrolysis of 3-aminopropyltriethoxysilane and polymerized on the surface of graphene oxide (GO) through an amidation reaction. Glutathione (GSH) was then modified to GO–POSS through a Michael addition reaction to obtain a GSH-functionalized GO–POSS composite (GPG). The structure and characteristics of the as-prepared GPG composite were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravity analysis, and surface charge analysis. The specific binding interactions between glutathione and GST gave GPG favorable adsorption selectivity towards GST, and other proteins did not affect GST adsorption. The adsorption behavior of GST on the GPG composite conformed to the Langmuir isotherm model, and the adsorption capacity of GST was high up to 364.94 mg g−1 under optimal conditions. The GPG-based solid-phase adsorption process was applied to the extraction of GST from a crude enzyme solution of pig liver, and high-purity GST was obtained via SDS-PAGE identification.
Interplay between Lignans and Gut Microbiota: Nutritional, Functional and Methodological Aspects
Authors: Baldi Simone , Tristán Asensi Marta , Pallecchi Marco , Sofi Francesco , Bartolucci Gianluca , Amedei Amedeo
Published: January 2023

Lignans are non-flavonoid polyphenols present in a wide range of foods frequently consumed in the Western world, such as seeds, vegetables and fruits, and beverages such as coffee, tea and wine. In particular, the human gut microbiota (GM) can convert dietary lignans into biologically active compounds, especially enterolignans (i.e., enterolactone and enterodiol), which play anti-inflammatory and anti-oxidant roles, act as estrogen receptor activators and modulate gene expression and/or enzyme activity. Interestingly, recent evidence documenting those dietary interventions involving foods enriched in lignans have shown beneficial and protective effects on various human pathologies, including colorectal and breast cancer and cardiovascular diseases. However, considering that more factors (e.g., diet, food transit time and intestinal redox state) can modulate the lignans bioactivation by GM, there are usually remarkable inter-individual differences in urine, fecal and blood concentrations of enterolignans; hence, precise and validated analytical methods, especially gas/liquid chromatography coupled to mass spectrometry, are needed for their accurate quantification. Therefore, this review aims to summarize the beneficial roles of enterolignans, their interaction with GM and the new methodological approaches developed for their evaluation in different biological samples, since they could be considered future promising nutraceuticals for the prevention of human chronic disorders.
Mineral Profile, Antioxidant, Anti-Inflammatory, Antibacterial, Anti-Urease and Anti-α-Amylase Activities of the Unripe Fruit Extracts of Pistacia atlantica
Authors: Benmohamed Mokhtar , Guenane Hamid , Messaoudi Mohammed , Zahnit Wafa , Egbuna Chukwuebuka , Sharifi-Rad Majid , Chouh Amina , Seghir Bachir Ben , Rebiai Abdelkrim , Boubekeur Sihem , Azli Tarek , Harrat Mohamed , Sawicka Barbara , Atanassova Maria , Yousfi Mohamed
Published: January 2023

Pistacia Atlantica in folk medicine is used by Algerian traditional healers for treating a wide variety of diseases and conditions including dyspepsia, digestive problems, peptic ulcers, and, in particular, inflammatory diseases. The present study aimed to assess the phytochemical composition, in vitro antioxidant activity (using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS+, and reducing power methods), enzyme inhibitory activity (towards α-amylase and urease), antibacterial activity, and in vivo anti-inflammatory activity of the unripe fruit extracts of Pistacia atlantica collected from different parts of the Djelfa region of Algeria. According to the findings, various aqueous extracts exhibited significant antioxidant and enzymatic activities in all tests, but showed that they have a weak inhibitory effect against all tested bacterial strains. Twenty-one minerals comprising both macro- and microelements (Ba, Br, Ca, Cl, Co, Cr, Cs, Eu, Fe, K, Mg, Mn, Mo, Na, Rb, Sb, Sc, Sr, Th, U, and Zn) were determined using the technique of neutron activation analysis (INAA). The result indicates that the concentration of the mineral element is close to the minimal FAO recommendation. In addition, the result revealed significant anti-inflammatory activities. The data generated can be a valuable source of information for the pharmaceutical industry and medical research. These results suggest that the unripe fruit extracts of Pistacia atlantica have an appropriate potential to be utilized across a wide range of contexts as an agent with multifunctional uses, as well as a natural remedy for other physiological diseases.
Phytochemical Analysis and Antiproliferative Activity of Ulex gallii Planch. (Fabaceae), a Medicinal Plant from Galicia (Spain)
Authors: Bada Lucía , Pereira Renato B. , Pereira David M. , Lores Marta , Celeiro María , Quezada Elías , Uriarte Eugenio , Gil-Longo José , Viña Dolores
Published: January 2023

The genus Ulex comprises thirteen accepted species of perennial shrubs in the family Fabaceae. In Galicia (Spain) many of these are considered spontaneous colonizing species, which are easy to establish and maintain. Among them, Ulex gallii Planch. is used in traditional medicine for the same anti-infective, hypotensive and diuretic purposes as Ulex europaeus L., which is the most studied species. Likewise, some studies have described the antitumoral properties of several species. However, there are few scientific studies that justify the use of Ulex gallii Planch. and nothing has been reported about its composition to date. In our study, the entire plant was extracted with methanol and the crude extract was subjected to liquid phase extraction with distinct solvents, yielding three fractions: hexane (H), dichloromethane (D) and methanol (M), which were subsequently fractionated. The dichloromethane (D5, D7 and D8) and methanol (M4) sub-fractions showed antiproliferative activity on A549 (lung cancer) and AGS (stomach cancer) cell lines, and caspase 3/7 activity assessment and DNA quantification were also performed. Targeted analysis via UHPLC-QToF, in combination with untargeted analysis via MS-Dial, MS-Finder and Global Natural Products Social Molecular Networking (GNPS), allowed us to tentatively identify different metabolites in these sub-fractions, mostly flavonoids, that might be involved in their antiproliferative activity.
The Effects of Propolis on Viral Respiratory Diseases
Authors: Ożarowski Marcin , Karpiński Tomasz M.
Published: January 2023

Propolis remains an interesting source of natural chemical compounds that show, among others, antibacterial, antifungal, antiviral, antioxidative and anti-inflammatory activities. Due to the growing incidence of respiratory tract infections caused by various pathogenic viruses, complementary methods of prevention and therapy supporting pharmacotherapy are constantly being sought out. The properties of propolis may be important in the prevention and treatment of respiratory tract diseases caused by viruses such as severe acute respiratory syndrome coronavirus 2, influenza viruses, the parainfluenza virus and rhinoviruses. One of the main challenges in recent years has been severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing COVID-19. Recently, an increasing number of studies are focusing on the activity of various propolis preparations against SARS-CoV-2 as an adjuvant treatment for this infection. Propolis has shown a few key mechanisms of anti-SARS-CoV-2 action such as: the inhibition of the interaction of the S1 spike protein and ACE-2 protein; decreasing the replication of viruses by diminishing the synthesis of RNA transcripts in cells; decreasing the particles of coronaviruses. The anti-viral effect is observed not only with extracts but also with the single biologically active compounds found in propolis (e.g., apigenin, caffeic acid, chrysin, kaempferol, quercetin). Moreover, propolis is effective in the treatment of hyperglycemia, which increases the risk of SARS-CoV-2 infections. The aim of the literature review was to summarize recent studies from the PubMed database evaluating the antiviral activity of propolis extracts in terms of prevention and the therapy of respiratory tract diseases (in vitro, in vivo, clinical trials). Based upon this review, it was found that in recent years studies have focused mainly on the assessment of the effectiveness of propolis and its chemical components against COVID-19. Propolis exerts wide-spectrum antimicrobial activities; thus, propolis extracts can be an effective option in the prevention and treatment of co-infections associated with diseases of the respiratory tract.
Comprehensive Review of Recent Advances in Chiral A-Ring Flavonoid Containing Compounds: Structure, Bioactivities, and Synthesis
Authors: Zhang Changyue , Liu Yanzhi , Liu Xiaojing , Chen Xiaochuan , Chen Ruijiao
Published: January 2023

Flavonoids are a group of natural polyphenolic substances that are abundant in vegetables, fruits, grains, and tea. Chiral A-ring-containing flavonoids are an important group of natural flavonoid derivatives applicable in a wide range of biological activities such as, cytotoxic, anti-inflammatory, anti-microbial, antioxidant, and enzyme inhibition. The desirable development of chiral A-ring-containing flavonoids by isolation, semi-synthesis or total synthesis in a short duration proves their great value in medicinal chemistry research. In this review, the research progress of chiral A-ring-containing flavonoids, including isolation and extraction, structural identification, pharmacological activities, and synthetic methods, is comprehensively and systematically summarized. Furthermore, we provide suggestions for future research on the synthesis and biomedical applications of flavonoids.
A DFT Study of Alkaline Earth Metal-Doped FAPbI3 (111) and (100) Surfaces
Authors: RaeisianAsl Maryam , Sarabadani Tafreshi Saeedeh , de Leeuw Nora H.
Published: January 2023

Density functional theory calculations have been performed to study the effect of replacing lead by alkaline earth metals on the stability, electronic and optical properties of the formamidinium lead triiodide (FAPbI3) (111) and (100) surfaces with different terminations in the form of FAPb1-xAExI3 structures, where AE is Be, Mg or Ca. It is revealed that the (111) surface is more stable, indicating metallic characteristics. The (100) surfaces exhibit a suitable bandgap of around 1.309 and 1.623 eV for PbI5 and PbI6 terminations, respectively. Increases in the bandgaps as a result of Mg- and Ca-doping of the (100) surface were particularly noted in FAPb0.96Ca0.04I3 and FAPb0.8Ca0.2I3 with bandgaps of 1.459 and 1.468 eV, respectively. In the presence of Be, the band gap reduces critically by about 0.315 eV in the FAPb0.95Be0.05I3 structure, while increasing by 0.096 eV in FAPb0.96Be0.04I3. Optimal absorption, high extinction coefficient and light harvesting efficiency were achieved for plain and doped (100) surfaces in the visible and near UV regions. In order to improve the optical properties of the (111)-PbI3 surface in initial visible areas, we suggest calcium-doping in this surface to produce FAPb0.96Ca0.04I3, FAPb0.92Ca0.08I3, and FAPb0.88Ca0.12I3 structures.
Analytical Validation of SOD2 Genotyping
Authors: Debeljak Marija , Riel Stacy , Lin Ming-Tseh , Eshleman James R. , Paller Channing J.
Published: January 2023

Manganese superoxide dismutase-2 (SOD2) plays a crucial role in cells’ protection against mitochondrial oxidative damage. A genetic polymorphism in the mitochondrial targeting sequence of the SOD2 gene has been implicated in various diseases, including prostate cancer. Paller et al. have shown an increase in prostate-specific antigen (PSA) doubling time in patients with the Ala/Ala (wildtype) genotype when treated with pomegranate/grape extract antioxidants. We developed and validated a pyrosequencing assay that detects the common germline SOD2 SNP (rs_4880) with the aim of identifying men with castrate-resistant prostate cancer eligible for an antioxidant therapy clinical trial. We first selected 37 samples from the 1000 genomes study with known genotypes determined using Illumina-based sequencing and confirmed them by Sanger sequencing. In a blinded design, we then performed the new pyrosequencing assay on these samples and assigned genotypes. Genotypes for all 37 samples (13 homozygous Ala, 12 heterozygous Ala/Val, and 12 homozygous Val) were all concordant by pyrosequencing. The pyrosequencing assay has been live since May 2018 and has proven to be robust and accurate.
Ultrasensitive Luteolin Electrochemical Sensor Based on Novel Lamellar CuZn@ Nitrogen-Containing Carbon Nanosheets
Authors: Li Yuhong , Yang Yaqi , Li Jiejun , Zhang Li , Zhao Pengcheng , Fei Junjie , Xie Yixi
Published: January 2023

The Cu/Zn-zeolitic imidazolate framework (Cu/Zn-ZIF) was synthesized using the traditional hydrothermal method, and its surface morphology was controlled by adding polyvinylpyrrolidone (PVP) during its synthesis. It was then calcined at 800 °C to form the nitrogen-containing carbon material CuZn@NC, which improved the electron transfer rate. Scanning electron microscopy (SEM), X-ray crystal diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were used to investigate the surface morphology and structure. Finally, the electrochemical sensing platform for luteolin was effectively constructed by changing the metal–ion ratio during synthesis to achieve the most suitable electrode material. The sensor platform detects luteolin well, with an operating curve equation of Ip (A) = 0.0571C (nM) − 1.2913 and a minimum detection limit of 15 nM, and the platform has been successfully employed for luteolin detection in real samples.
Formulation, Optimization and Evaluation of Cytarabine-Loaded Iron Oxide Nanoparticles: From In Vitro to In Vivo Evaluation of Anticancer Activity
Authors: Fule Ritesh , Kaleem Mohammed , Asar Turky Omar , Rashid Md Abdur , Shaik Rasheed A. , Eid Basma G. , Nasrullah Mohammed Z. , Ahmad Aftab , Kazmi Imran
Published: January 2023

Innovative drug delivery systems based on iron oxide nanoparticles (INPs) has generated a lot of interest worldwide and have prime biomedical benefits in anticancer therapy. There are still issues reported regarding the stability, absorption, and toxicity of iron oxide nanoparticles (INPs) when administered due to its rapid surface oxidation and agglomeration with blood proteins. To solve this problem, we have synthesized trehalose-coated stabilized iron oxide nanoparticles (TINPs) by a co-precipitation technique. The surface coating of INPs with trehalose helps to improve the stability, prevents protein binding, and increase absorption uptake inside the body. Developed TINPs was then loaded with anticancer drug cytarabine by chemical crosslinking encapsulation method using suitable solvent. Engineered cytarabine-loaded trehalose-coated stabilized iron oxide nanoparticles (CY-TINPs) were optimized for particle size, zeta potential (−13.03 mV), and solid-state characterization such as differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), and transmission electron microscope (TEM) studies. The particle size of 50 nm was achieved for developed CY-TINPs. The developed CY-TINPs was further evaluated for in vitro cell line investigations which confirmed potential cytotoxic activity. Developed CY-TINPs show remarkable enhancement in in vivo pharmacokinetic parameters Cmax as 425.26 ± 2.11 and AUC0–72 as 11,546.64 ± 139.82 as compared to pure drug. Compared to traditional drug delivery, the CY-TINPs formulation can effectively delay release, improve bioavailability, and boost cytotoxic activity against tumors.
Kinetics, Electronic Properties of Filled Carbon Nanotubes Investigated with Spectroscopy for Applications
Authors: Kharlamova Marianna V.
Published: January 2023

The paper is dedicated to the discussion of kinetics of growth, and electronic properties of filled carbon nanotubes investigated by spectroscopy for applications. The paper starts with discussion of growth of carbon nanotubes inside metallocene-filled carbon nanotubes. Nickelocene, cobaltocene are considered for growth of carbon nanotubes. Then, the investigations of filled carbon nanotubes by four spectroscopic techniques are discussed. Among them are Raman spectroscopy, near edge X-ray absorption fine-structure spectroscopy, photoemission spectroscopy, optical absorption spectroscopy. It is discussed that metal halogenides, metal chalcogenides, metals lead to changes in electronic structure of nanotubes with n- or p-doping. The filling of carbon nanotubes with different organic and inorganic substances results in many promising applications. This review adds significant contribution to understanding of the kinetics and electronic properties of filled SWCNTs with considering new results of recent investigations. Challenges in various fields are analyzed and summarized, which shows the author’s viewpoint of progress in the spectroscopy of filled SWCNTs. This is a valuable step toward applications of filled SWCNTs and transfer of existing ideas from lab to industrial scale.
Silicon Nanowire-Assisted High Uniform Arrayed Waveguide Grating
Authors: Yuan Shuo , Feng Jijun , Yu Zhiheng , Chen Jian , Liu Haipeng , Chen Yishu , Guo Song , Huang Fengli , Akimoto Ryoichi , Zeng Heping
Published: January 2023

Determining how to improve the non-uniformity of arrayed waveguide grating (AWG) is of great significance for dense wavelength division multiplexing (DWDM) systems. In this work, a silicon nanowire-assisted AWG structure is proposed, which can achieve high uniformity with a low insertion loss. The article compares the effect of nanowire number and shape on uniformity and insertion loss, finding that double nanowires provide the best performance. Double nanowires with a width of 230 nm and length of 3.5 μm can consist of a slot configuration between arrayed waveguides, both connecting to the star coupler and spacing 165 nm from the waveguides. Compared with conventional 8- and 16-channel AWGs with channel spacing of 200 GHz, the non-uniformity of the presented structure can be improved from 1.09 and 1.6 dB to 0.24 and 0.63 dB, respectively. The overall footprint of the device would remain identical, which is 276 × 299 or 258 × 303 μm2 for the 8- or 16-channel AWG. The present high uniformity design is simple and easy to fabricate without any additional insertion loss, which is expected to be widely applied in the highly integrated DWDM systems.
Towards Efficient Electrically-Driven Deep UVC Lasing: Challenges and Opportunities
Authors: Nikishin Sergey , Bernussi Ayrton , Karpov Sergey
Published: January 2023

The major issues confronting the performance of deep-UV (DUV) laser diodes (LDs) are reviewed along with the different approaches aimed at performance improvement. The impact of threading dislocations on the laser threshold current, limitations on heavy n- and p-doping in Al-rich AlGaN alloys, unavoidable electron leakage into the p-layers of (0001) LD structures, implementation of tunnel junctions, and non-uniform hole injection into multiple quantum wells in the active region are discussed. Special attention is paid to the current status of n- and p-type doping and threading dislocation density reduction, both being the factors largely determining the performance of DUV-LDs. It is shown that most of the above problems originate from intrinsic properties of the wide-bandgap AlGaN semiconductors, which emphasizes their fundamental role in the limitation of deep-UV LD performance. Among various remedies, novel promising technological and design approaches, such as high-temperature face-to-face annealing and distributed polarization doping, are discussed. Whenever possible, we provided a comparison between the growth capabilities of MOVPE and MBE techniques to fabricate DUV-LD structures.