bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Effect of Vegetation Index Based on Multiple Tree-Ring Parameters in the Central Tianshan Mountains
Authors: Song Jinghui , Zhang Tongwen , Fan Yuting , Liu Yan , Yu Shulong , Jiang Shengxia , Guo Dong , Hou Tianhao , Guo Kailong
Published: November 2023

Combining tree ring data with remote sensing data can help to gain a deeper understanding of the driving factors that influence vegetation change, identify climate events that lead to vegetation change, and improve the parameters of global vegetation index reconstruction models. However, it is currently not well understood how climate change at different elevations in the central Tianshan Mountains affects radial tree growth and the dynamics of forest canopy growth. We selected Schrenk spruce (Picea schrenkiana) tree core samples from different elevations in the central Tianshan Mountains. We analyzed the relationships of various tree-ring parameters, including tree-ring width, maximum latewood density (MXD), and minimum earlywood density (MID) chronologies, with 1982–2012 GIMMS (Global Inventory Modelling and Mapping Studies) NDVI (Normalized Difference Vegetation Index), 2001–2012 MODIS (moderate resolution imaging spectroradiometer) NDVI, and meteorological data. (1) There were strong correlations between tree-ring width chronologies and the lowest temperatures, especially in July. Tree-ring width chronologies at higher altitudes were positively correlated with temperature; the opposite pattern was observed at lower altitudes. MID chronologies were positively correlated with July temperature in high-altitude areas and mean temperature and highest temperature from May to September in low-altitude areas, and negatively correlated with precipitation during this period. MXD chronologies were mainly negatively correlated with precipitation. MXD chronologies were mainly positively correlated with temperature in April and May. (2) The correlations between MXD chronologies at each sampling point and NDVI in each month of the growing season were strong. Both MID and MXD chronologies were negatively correlated with GIMMS NDVI in July. The overall correlations between tree-ring parameters and MODIS NDVI were stronger than the correlations between tree-ring parameters and GIMMS NDVI in high-altitude areas; the opposite pattern was observed in low-altitude areas. Drought stress may be the main factor affecting tree ring parameters and NDVI. In the future, we should combine tree ring parameters with vegetation index to investigate a larger scale of forests.
Knowledge Distillation-Based GPS Spoofing Detection for Small UAV
Authors: Ren Yingying , Restivo Ryan D. , Tan Wenkai , Wang Jian , Liu Yongxin , Jiang Bin , Wang Huihui , Song Houbing
Published: November 2023

As a core component of small unmanned aerial vehicles (UAVs), GPS is playing a critical role in providing localization for UAV navigation. UAVs are an important factor in the large-scale deployment of the Internet of Things (IoT) and cyber–physical systems (CPS). However, GPS is vulnerable to spoofing attacks that can mislead a UAV to fly into a sensitive area and threaten public safety and private security. The conventional spoofing detection methods need too much overhead, which stops efficient detection from working in a computation-constrained UAV and provides an efficient response to attacks. In this paper, we propose a novel approach to obtain a lightweight detection model in the UAV system so that GPS spoofing attacks can be detected from a long distance. With long-short term memory (LSTM), we propose a lightweight detection model on the ground control stations, and then we distill it into a compact size that is able to run in the control system of the UAV with knowledge distillation. The experimental results show that our lightweight detection algorithm runs in UAV systems reliably and can achieve good performance in GPS spoofing detection.
Snapshot of the Probiotic Potential of Kluveromyces marxianus DMKU-1042 Using a Comparative Probiogenomics Approach
Authors: Ullah Mati , Rizwan Muhammad , Raza Ali , Xia Yutong , Han Jianda , Ma Yi , Chen Huayou
Published: November 2023

Kluyveromyces marxianus is a rapidly growing thermotolerant yeast that secretes a variety of lytic enzymes, utilizes different sugars, and produces ethanol. The probiotic potential of this yeast has not been well explored. To evaluate its probiotic potential, the yeast strain Kluyveromyces marxianus DMKU3-1042 was analyzed using next-generation sequencing technology. Analysis of the genomes showed that the yeast isolates had a GC content of 40.10–40.59%. The isolates had many genes related to glycerol and mannose metabolism, as well as genes for acetoin and butanediol metabolism, acetolactate synthase subunits, and lactic acid fermentation. The strain isolates were also found to possess genes for the synthesis of different vitamins and Coenzyme A. Genes related to heat and hyperosmotic shock tolerance, as well as protection against reactive oxygen species were also found. Additionally, the isolates contained genes for the synthesis of lysine, threonine, methionine, and cysteine, as well as genes with anticoagulation and anti-inflammatory properties. Based on our analysis, we concluded that the strain DMKU3-1042 possesses probiotic properties that make it suitable for use in food and feed supplementation.
Toxicological Profile and Anti-Inflammatory Effect of Mucoadhesive Gel from Residues of Agave sisalana and Punica granatum
Authors: Fracasso Júlia Amanda Rodrigues , Sikina Ingrid Yuri Galindo , da Costa Luísa Taynara Silvério , Guarnier Lucas Pires , Ribeiro-Paes João Tadeu , de Ferreira Fernando Yutaka , de Almeida Luan Victor Coelho , de Castro Silva Beatriz , de Barros Barbosa Débora , Ximenes Valdecir Farias , Venkli Desirre , Viel Amanda Martins , dos Santos Lucinéia
Published: November 2023

Inflammation is a natural protective reaction of the body against endogenous and exogenous damage, such as tissue injuries, trauma, and infections. Thus, when the response is adequate, inflammation becomes a defense mechanism to repair damaged tissue, whereas when the response is inadequate and persistent, the increase in inflammatory cells, cytosines, and chymosins impair tissue regeneration and promote a response harmful to the organism. One example is chronic tissue inflammation, in which a simple lesion can progress to ulcers and even necrosis. In this situation, the anti-inflammatory medications available in therapy are not always effective. For this reason, the search for new treatments, developed from medicinal plants, has increased. In this direction, the plants Agave sisalana (sisal) and Punica granatum (pomegranate) are rich in saponins, which are secondary metabolites known for their therapeutic properties, including anti-inflammatory effects. Although Brazil is the world’s leading sisal producer, approximately 95% of the leaves are discarded after fiber extraction. Similarly, pomegranate peel waste is abundant in Brazil. To address the need for safe and effective anti-inflammatory treatments, this study aimed to create a topical mucoadhesive gel containing a combination of sisal (RS) and pomegranate residue (PR) extracts. In vitro experiments examined isolated and combined extracts, as well as the resulting formulation, focusing on (1) a phytochemical analysis (total saponin content); (2) cytotoxicity (MTT assay); and (3) a pharmacological assessment of anti-inflammatory activity (phagocytosis, macrophage spreading, and membrane stability). The results revealed saponin concentrations in grams per 100 g of dry extract as follows: SR—29.91 ± 0.33, PR—15.83 ± 0.93, association (A)—22.99 ± 0.01, base gel (G1)—0.00 ± 0.00, and association gel (G2)—0.52 ± 0.05. In MTT tests for isolated extracts, cytotoxicity values (µg/mL) were 3757.00 for SR and 2064.91 for PR. Conversely, A and G2 exhibited no cytotoxicity, with increased cell viability over time. All three anti-inflammatory tests confirmed the presence of this activity in SR, PR, and A. Notably, G2 demonstrated an anti-inflammatory effect comparable to dexamethasone. In conclusion, the gel containing SR and PR (i.e., A) holds promise as a novel herbal anti-inflammatory treatment. Its development could yield economic, social, and environmental benefits by utilizing discarded materials in Brazil.
The Genetic Diversity of White-Backed Planthoppers (Sogatella furcifera) between Myanmar and Yunnan Province of China
Authors: Liu Yue , Chan Khin Nyein , Li Xiangyong , Zhao Xueqing , Chu Dong , Yin Yanqiong , Liu Ying , Chen Aidong
Published: November 2023

In order to clarify the migration route and the source of white-backed planthopper (WBPH) (Sogatella furcifera) between Myanmar and Yunnan Province, China, we collected six populations throughout Myanmar and five populations around the border areas in Yunnan Province, China. A total of 790 base pairs in the mtDNA COI genes from 416 individuals were obtained. A total of 43 haplotypes were identified, among which 37 were unique haplotypes, and the remaining 6 were shared among different populations. Two common shared haplotypes (H_1 and H_2) had a widespread distribution in all populations and accounted for 88.8% of the total haplotype frequency, suggesting a high-level gene flow among the Myanmar and Yunnan populations. Bayesian skyline plot (BSP) analysis results indicated that the effective population size of WBPH expanded between about 10,000 and 7000 years ago, and S. furcifera might follow the post-LGM (Last Glacial Maximum) expansion pattern. Based on the total migrant (Nem) value, it can be deduced that north and northeast Myanmar were the primary migration sources for WBPH populations in the southwest and south Yunnan regions. This study aims to contribute to the sustainable regional management of this important rice pest and provide new insights into the genetic diversity of WBPH in Southeast Asia.
A Pilot Study on the Association of Internet Use with Sports Practice and Sex in Italian Adolescents
Authors: Toselli Stefania , Grigoletto Alessia , Zaccagni Luciana , Gualdi-Russo Emanuela , Rinaldo Natascia
Published: November 2023

The use of the Internet has several positive functions, but at the same time it also represents a health risk, especially for adolescents. The increased use of the Internet in recent decades has also been linked to an increase in problematic Internet use, which has now become a global social issue. During the COVID-19 pandemic, Internet use increased even more and influenced adolescents’ habits, leading to an increase in sedentary behaviors. The aim of this study was to understand whether Internet addiction differed between sportive and nonsportive adolescents and between males and females. We analyzed two samples of adolescents, with different behaviors regarding sports practice. Internet addiction was assessed by the administration of a validated questionnaire (UADI) via an online survey. Although all the mean values of both the subgroups fell into the “non-pathological” category, differences between sports groups and sexes were found, with different trends for each one of the five dimensions of Internet use. In conclusion, sportive adolescents generally showed less severe Internet addiction; therefore, encouraging sports practice can help to fight wrong habits related to a sedentary lifestyle. The promotion of sports practice in these ages is therefore a priority aspect, especially in females, who showed a general predisposition to escape from reality in comparison to males.
Genome-Wide Identification of Mango (Mangifera indica L.) MADS-Box Genes Related to Fruit Ripening
Authors: Zheng Bin , Wang Songbiao , Wu Hongxia , Ma Xiaowei , Xu Wentian , Xie Kunliang , Shangguan Lingfei , Fang Jinggui
Published: November 2023

MADS-box genes play a crucial role in fruit ripening, yet limited research has been conducted on mango. Based on the conserved domains of this gene family, 84 MADS-box genes were identified in the mango genome, including 22 type I and 62 type II MADS-box genes. Gene duplication analysis revealed that both tandem duplication and segmental replication significantly contributed to the expansion of MADS-box genes in the mango genome, with purifying selection playing a vital role in the segmental duplication events within the MiMADS gene family. Cis-acting element analysis demonstrated that most MiMADS genes were hormonally regulated and participated in the growth, development, and stress resistance of mango fruit. Moreover, through expression pattern analysis and phylogenetic tree construction, we identified six MiMADS genes belonging to the SEP1 subfamily and two belonging to the AG subfamily as potential candidates involved in mango ripening regulation. Notably, Mi08g17750 and Mi04g18430 from the SEP1 subfamily were identified as key regulators inhibiting mango fruit maturation; their interaction network was also analyzed. These findings provide a foundation for further investigation into the regulatory mechanisms underlying mango ripening.
Impact of Prescribed Exercise on the Physical and Cognitive Health of Adults with Down Syndrome: The MinDSets Study
Authors: Merzbach Viviane , Ferrandino Michael , Gernigon Marie , Marques Pinto Jorge , Scruton Adrian , Gordon Dan
Published: November 2023

The duplication of chromosome 21, as evidenced in Down Syndrome (DS), has been linked to contraindications to health, such as chronotropic and respiratory incompetence, neuromuscular conditions, and impaired cognitive functioning. The purpose of this study was to examine the effects of eight weeks of prescribed exercise and/or cognitive training on the physical and cognitive health of adults with DS. Eighty-three participants (age 27.1 ± 8.0 years) across five continents participated. Physical fitness was assessed using a modified version of the six-minute walk test (6MWT), while cognitive and executive functions were assessed using the Corsi block test, the Sustained-Attention-To-Response Task (SART), and the Stroop task (STROOP). All were completed pre- and post-intervention. Participants were assigned to eight weeks of either exercise (EXE), 3 × 30 min of walking/jogging per week, cognitive training (COG) 6 × ~20 min per week, a combined group (COM), and a control group (CON) engaging in no intervention. 6MWT distance increased by 11.4% for EXE and 9.9% for COM (p < 0.05). For SART, there were positive significant interactions between the number of correct and incorrect responses from pre- to post-intervention when participants were asked to refrain from a response (NO-GO-trials) across all experimental groups (p < 0.05). There were positive significant interactions in the number of correct, incorrect, and timeout incompatible responses for STROOP in EXE, COG, and COM (p < 0.05). Walking generated a cognitive load attributed to heightened levels of vigilance and decision-making, suggesting that exercise should be adopted within the DS community to promote physical and cognitive well-being.
Race- and Gender-Specific Associations between Neighborhood-Level Socioeconomic Status and Body Mass Index: Evidence from the Southern Community Cohort Study
Authors: Giurini Lauren , Lipworth Loren , Murff Harvey J. , Zheng Wei , Warren Andersen Shaneda
Published: November 2023

Obesity and a low socioeconomic status (SES), measured at the neighborhood level, are more common among Americans of Black race and with a low individual-level SES. We examined the association between the neighborhood SES and body mass index (BMI) using data from 80,970 participants in the Southern Community Cohort Study, a cohort that oversamples Black and low-SES participants. BMI (kg/m2) was examined both continuously and categorically using cut points defined by the CDC. Neighborhood SES was measured using a neighborhood deprivation index composed of census-tract variables in the domains of education, employment, occupation, housing, and poverty. Generally, the participants in lower-SES neighborhoods were more likely to have a higher BMI and to be considered obese. We found effect modification by race and sex, where the neighborhood-BMI association was most apparent in White female participants in all the quintiles of the neighborhood SES (ORQ2 = 1.55, 95%CI = 1.34, 1.78; ORQ3 = 1.71, 95%CI = 1.48, 1.98; ORQ4 = 1.76, 95%CI = 1.52, 2.03; ORQ5 = 1.64, 95%SE = 1.39, 1.93). Conversely, the neighborhood-BMI association was mostly null in Black male participants (ORQ2 = 0.91, 95%CI = 0.72, 1.15; ORQ3 = 1.05, 95%CI = 0.84, 1.31; βQ4 = 1.00, 95%CI = 0.81, 1.23; ORQ5 = 0.76, 95%CI = 0.63, 0.93). Within all the subgroups, the associations were attenuated or null in participants residing in the lowest-SES neighborhoods. These findings suggest that the associations between the neighborhood SES and BMI vary, and that other factors aside from the neighborhood SES may better predict the BMI in Black and low-SES groups.
Inflammatory Bowel Disease and Irritable Bowel Syndrome: What Differences in Mentalization Abilities? A Scoping Review
Authors: Benfante Agata , Cisarò Fabio , Ribaldone Davide Giuseppe , Castelli Lorys , Sandroni Nikolas , Romeo Annunziata
Published: November 2023

Mentalization is a psychological process that enables individuals to understand the self and others in terms of intentional mental states. The aim of this scoping review was to provide an overview of the findings on mentalization in patients with inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). A literature search, in line with the Preferred Reporting Items for Systematic Review and Meta-analysis Protocols extension for Scoping Review guidelines, was conducted in the following bibliographic databases: PubMed, PsycINFO, and Scopus. Databases were queried using the following strings (with Boolean operators): (“mentaliz*” OR “metacogniti*” OR “theory of mind” OR “ToM” OR “reflective function*”) AND (“irritable bowel syndrome” OR “IBS” OR “inflammatory bowel disease” OR “IBD”). In line with the eligibility criteria, seven articles were included. Results showed that no significant differences in metacognitive ability were found between patients in the IBD and IBS groups. This review revealed the mentalizing difficulties for patients with IBD and IBS. These results should be interpreted with caution since they are based on a few studies that used different instruments to assess mentalizing processes. Future studies are needed to clarify the role of mentalization in patients with these gastrointestinal conditions.
Mmp12 Is Translationally Regulated in Macrophages during the Course of Inflammation
Authors: Kuntschar Silvia , Cardamone Giulia , Klann Kevin , Bauer Rebekka , Meyer Sofie Patrizia , Raue Rebecca , Rappl Peter , Münch Christian , Brüne Bernhard , Schmid Tobias
Published: November 2023

Despite the importance of rapid adaptive responses in the course of inflammation and the notion that post-transcriptional regulation plays an important role herein, relevant translational alterations, especially during the resolution phase, remain largely elusive. In the present study, we analyzed translational changes in inflammatory bone marrow-derived macrophages upon resolution-promoting efferocytosis. Total RNA-sequencing confirmed that apoptotic cell phagocytosis induced a pro-resolution signature in LPS/IFNγ-stimulated macrophages (Mϕ). While inflammation-dependent transcriptional changes were relatively small between efferocytic and non-efferocytic Mϕ; considerable differences were observed at the level of de novo synthesized proteins. Interestingly, translationally regulated targets in response to inflammatory stimuli were mostly downregulated, with only minimal impact of efferocytosis. Amongst these targets, pro-resolving matrix metallopeptidase 12 (Mmp12) was identified as a translationally repressed candidate during early inflammation that recovered during the resolution phase. Functionally, reduced MMP12 production enhanced matrix-dependent migration of Mϕ. Conclusively, translational control of MMP12 emerged as an efficient strategy to alter the migratory properties of Mϕ throughout the inflammatory response, enabling Mϕ migration within the early inflammatory phase while restricting migration during the resolution phase.
Neuronal Scaffold Protein ARMS Interacts with Synaptotagmin-4 C2AB through the Ankyrin Repeat Domain with an Unexpected Mode
Authors: Zhang Fa , Chen Jiasheng , Li Yahong , Ye Jin , Wang Chao
Published: November 2023

The ankyrin repeat-rich membrane spanning (ARMS), a transmembrane neuronal scaffold protein, plays a fundamental role in neuronal physiology, including neuronal development, polarity, differentiation, survival and angiogenesis, through interactions with diverse partners. Previous studies have shown that the ARMS negatively regulates brain-derived neurotrophic factor (BDNF) secretion by interacting with Synaptotagmin-4 (Syt4), thereby affecting neurogenesis and the development and function of the nervous system. However, the molecular mechanisms of the ARMS/Syt4 complex assembly remain unclear. Here, we confirmed that the ARMS directly interacts with Syt4 through its N-terminal ankyrin repeats 1–8. Unexpectedly, both the C2A and C2B domains of Syt4 are necessary for binding with the ARMS. We then combined the predicted complex structural models from AlphaFold2 with systematic biochemical analyses using point mutagenesis to underline the molecular basis of ARMS/Syt4 complex formation and to identify two conserved residues, E15 and W72, of the ARMS, as essential residues mediating the assembly of the complex. Furthermore, we showed that ARMS proteins are unable to interact with Syt1 or Syt3, indicating that the interaction between ARMS and Syt4 is specific. Taken together, the findings from this study provide biochemical details on the interaction between the ARMS and Syt4, thereby offering a biochemical basis for the further understanding of the potential mechanisms and functional implications of the ARMS/Syt4 complex formation, especially with regard to the modulation of BDNF secretion and associated neuropathies.
Comparative RNA-Seq Analysis Revealed Tissue-Specific Splicing Variations during the Generation of the PDX Model
Authors: Lee Eun Ji , Noh Seung-Jae , Choi Huiseon , Kim Min Woo , Kim Su Jin , Seo Yeon Ah , Jeong Ji Eun , Shin Inkyung , Kim Jong-Seok , Choi Jong-Kwon , Cho Dae-Yeon , Chang Suhwan
Published: November 2023

Tissue-specific gene expression generates fundamental differences in the function of each tissue and affects the characteristics of the tumors that are created as a result. However, it is unclear how much the tissue specificity is conserved during grafting of the primary tumor into an immune-compromised mouse model. Here, we performed a comparative RNA-seq analysis of four different primary-patient derived xenograft (PDX) tumors. The analysis revealed a conserved RNA biotype distribution of primary−PDX pairs, as revealed by previous works. Interestingly, we detected significant changes in the splicing pattern of PDX, which was mainly comprised of skipped exons. This was confirmed by splicing variant-specific RT-PCR analysis. On the other hand, the correlation analysis for the tissue-specific genes indicated overall strong positive correlations between the primary and PDX tumor pairs, with the exception of gastric cancer cases, which showed an inverse correlation. These data propose a tissue-specific change in splicing events during PDX formation as a variable factor that affects primary−PDX integrity.
Usable Security: A Systematic Literature Review
Authors: Di Nocera Francesco , Tempestini Giorgia , Orsini Matteo
Published: November 2023

Usable security involves designing security measures that accommodate users’ needs and behaviors. Balancing usability and security poses challenges: the more secure the systems, the less usable they will be. On the contrary, more usable systems will be less secure. Numerous studies have addressed this balance. These studies, spanning psychology and computer science/engineering, contribute diverse perspectives, necessitating a systematic review to understand strategies and findings in this area. This systematic literature review examined articles on usable security from 2005 to 2022. A total of 55 research studies were selected after evaluation. The studies have been broadly categorized into four main clusters, each addressing different aspects: (1) usability of authentication methods, (2) helping security developers improve usability, (3) design strategies for influencing user security behavior, and (4) formal models for usable security evaluation. Based on this review, we report that the field’s current state reveals a certain immaturity, with studies tending toward system comparisons rather than establishing robust design guidelines based on a thorough analysis of user behavior. A common theoretical and methodological background is one of the main areas for improvement in this area of research. Moreover, the absence of requirements for Usable security in almost all development contexts greatly discourages implementing good practices since the earlier stages of development.
Explainable Deep Learning Approach for Multi-Class Brain Magnetic Resonance Imaging Tumor Classification and Localization Using Gradient-Weighted Class Activation Mapping
Authors: Hussain Tahir , Shouno Hayaru
Published: November 2023

Brain tumors (BT) present a considerable global health concern because of their high mortality rates across diverse age groups. A delay in diagnosing BT can lead to death. Therefore, a timely and accurate diagnosis through magnetic resonance imaging (MRI) is crucial. A radiologist makes the final decision to identify the tumor through MRI. However, manual assessments are flawed, time-consuming, and rely on experienced radiologists or neurologists to identify and diagnose a BT. Computer-aided classification models often lack performance and explainability for clinical translation, particularly in neuroscience research, resulting in physicians perceiving the model results as inadequate due to the black box model. Explainable deep learning (XDL) can advance neuroscientific research and healthcare tasks. To enhance the explainability of deep learning (DL) and provide diagnostic support, we propose a new classification and localization model, combining existing methods to enhance the explainability of DL and provide diagnostic support. We adopt a pre-trained visual geometry group (pre-trained-VGG-19), scratch-VGG-19, and EfficientNet model that runs a modified form of the class activation mapping (CAM), gradient-weighted class activation mapping (Grad-CAM) and Grad-CAM++ algorithms. These algorithms, introduced into a convolutional neural network (CNN), uncover a crucial part of the classification and can provide an explanatory interface for diagnosing BT. The experimental results demonstrate that the pre-trained-VGG-19 with Grad-CAM provides better classification and visualization results than the scratch-VGG-19, EfficientNet, and cutting-edge DL techniques regarding visual and quantitative evaluations with increased accuracy. The proposed approach may contribute to reducing the diagnostic uncertainty and validating BT classification.
Spinal Involvement in Patients with Chronic Non-Bacterial Osteomyelitis (CNO): An Analysis of Distinctive Imaging Features
Authors: Byrdy-Daca Marta , Duczkowski Marek , Sudoł-Szopińska Iwona , Żelewska Marta , Piłat Krzysztof , Daca Filip , Nieciecki Michał , Sztwiertnia Paweł , Walecki Jerzy , Cieszanowski Andrzej , Świątkowski Jan , Bereźniak Marlena , Sułkowska Katarzyna , Czubak Jarosław , Gołębiowski Marek , Palczewski Piotr
Published: November 2023

Spinal involvement by chronic non-bacterial osteomyelitis (CNO) has been increasingly reported in recent years, often being presented as a diagnostic dilemma requiring differential diagnosis with bacterial spondylodiscitis and/or neoplasia. This study was aimed at identifying the imaging features of CNO facilitating its differentiation from other spinal diseases. Two radiologists assessed the imaging studies of 45 patients (16 male and 29 female, aged from 6 to 75 years, 15 children) with CNO collected from 5 referential centers. Spinal lesions were found in 17 patients (2 children and 15 adults), most often in the thoracic spine. In children, the lesions involved short segments with a destruction of vertebral bodies. In adults, the main findings were prominent bone marrow edema and osteosclerosis, endplate irregularities, and ankylosing lesions extending over long segments; paraspinal inflammation was mild and abscesses were not observed. In both children and adults, the involvement of posterior elements (costovertebral and facet joints) emerged as an important discriminator between CNO and neoplasia/other inflammatory conditions. In conclusion, a careful inspection of imaging studies may help to reduce the number of biopsies performed in the diagnostic process of CNO.
COPD after “Tabouna” Exposure: A Distinct Phenotype in Tunisian Women?
Authors: Hamdi Besma , Louhaichi Sabrine , Jebali Mohamed Aymen , Schlemmer Frédéric , Maitre Bernard , Hamzaoui Agnes
Published: November 2023

Background: COPD due to exposure to combustible biomass is an increasingly recognized phenotype, particularly among women who use traditional ovens, known as ‘Tabouna’, for baking bread. This paper aims to investigate the clinical and functional characteristics of COPD in Tunisian female patients attributed to the use of ‘Tabouna’. Methods: A retrospective single-center cohort study was conducted on patients recruited from the Department of Respiratory Disease at A. Mami Hospital, who were diagnosed with COPD between January 2014 and December 2022. The diagnosis of COPD adhered to the standards defined in GOLD 2022. Results: Out of the 95 women included in the study, 48 (50.5%) were exposed to tobacco smoke, while 47 (49.5%) were exposed to the ‘Tabouna’. The median age was 70.4 ± 11.5 years, ranging from 40 to 95 years. Patients exposed to biomass were notably older, with a median age of 75.4 compared to 64.6 (p = 0.04). A significant association was observed between COPD and biomass smoke exposure, both in women residing in rural and urban areas (p = 0.006). The frequency of patients exposed to biomass with comorbidities was higher than in the group exposed to tobacco, but only hypertension showed statistically significant results (p = 0.01). Tobacco smoke induced more impairment in lung function than biomass in the group with FEV1 ≤ 30% (p = 0.04). Long-acting muscarinic antagonists were more commonly prescribed to smokers (p = 0.04). Serious complications such as chronic respiratory failure and intensive care admissions were similar in both groups (p = 0.8 and 0.4). Conclusions: COPD in women after exposure to the ‘Tabouna’ was observed in older patients and characterized by delayed diagnosis. Despite these clinical differences, poor COPD outcomes were similar in both groups.
Laryngopharyngeal Reflux Scoring in a Pediatric Population
Authors: Abičić Ivan , Čović Marina , Zjalić Milorad , Bakula Marina , Marjanović Ksenija , Šestak Anamarija , Dmitrović Branko , Mendeš Tihana , Smolić Martina , Wu George Y. , Mihalj Hrvoje , Zubčić Željko , Včeva Andrijana
Published: November 2023

In recent years, the prevalence of laryngopharyngeal reflux has risen, especially among pediatric patients. The diagnosis of laryngopharyngeal reflux relies on patient history and clinical assessment using the Reflux Finding Score and Reflux Symptom Index as crucial diagnostic tools. Some studies have proposed a link between pepsin and laryngopharyngeal reflux, potentially triggering palatine tonsil hypertrophy. Our study aimed to investigate the correlation between laryngeal and pharyngeal manifestations of laryngopharyngeal reflux through two questionnaires and the presence of pepsin in saliva and palatine tonsils in a pediatric population. Pepsin in saliva was detected using a Western blot method, while immunohistochemistry assessed its presence in palatine tonsils. Although no statistically significant differences in Reflux Finding Score and Reflux Symptom Index were found between the immunohistochemistry-positive (IHC-positive) and immunohistochemistry-negative (IHC-negative) groups, median reflux symptom index and Reflux Finding Score values consistently trended higher in the IHC-positive group. This suggests a potential connection between elevated index values and pepsin presence in tonsillar tissue. Further investigations are essential to fully comprehend the clinical implications of these findings.
Parkinson’s Disease Non-Motor Subtypes Classification in a Group of Slovenian Patients: Actuarial vs. Data-Driven Approach
Authors: Petrijan Timotej , Zmazek Jan , Menih Marija
Published: November 2023

Background and purpose: The aim of this study was to examine the risk factors, prodromal symptoms, non-motor symptoms (NMS), and motor symptoms (MS) in different Parkinson’s disease (PD) non-motor subtypes, classified using newly established criteria and a data-driven approach. Methods: A total of 168 patients with idiopathic PD underwent comprehensive NMS and MS examinations. NMS were assessed by the Non-Motor Symptom Scale (NMSS), Montreal Cognitive Assessment (MoCA), Hamilton Depression Scale (HAM-D), Hamilton Anxiety Rating Scale (HAM-A), REM Sleep Behavior Disorder Screening Questionnaire (RBDSQ), Epworth Sleepiness Scale (ESS), Starkstein Apathy Scale (SAS) and Fatigue Severity Scale (FSS). Motor subtypes were classified based on Stebbins’ method. Patients were classified into groups of three NMS subtypes (cortical, limbic, and brainstem) based on the newly designed inclusion criteria. Further, data-driven clustering was performed as an alternative, statistical learning-based classification approach. The two classification approaches were compared for consistency. Results: We identified 38 (22.6%) patients with the cortical subtype, 48 (28.6%) with the limbic, and 82 (48.8%) patients with the brainstem NMS PD subtype. Using a data-driven approach, we identified five different clusters. Three corresponded to the cortical, limbic, and brainstem subtypes, while the two additional clusters may have represented patients with early and advanced PD. Pearson chi-square test of independence revealed that a priori classification and cluster membership were significantly related to one another with a large effect size (χ2(8) = 175.001, p < 0.001, Cramer’s V = 0.722). The demographic and clinical profiles differed between NMS subtypes and clusters. Conclusion: Using the actuarial and clustering approach, marked differences between individual NMS subtypes were found. The newly established criteria have potential as a simplified tool for future clinical research of NMS subtypes of Parkinson’s disease.
Improvement of Groin Pain in a Football Player with Femoroacetabular Impingement via a Correction of the Pelvic Position—A Case Report
Authors: Ludwig Oliver , Schneider Günther , Kelm Jens
Published: November 2023

Background: Femoroacetabular impingement is one possible cause for groin pain and can lead to long periods of absence for football players. In cam impingement, the end-grade position of the leg at kicking makes the hip particularly prone to faulty contact between the acetabulum and the femoral head. Studies suggest that the resting position of the pelvis in the sagittal plane may have an important role in the biomechanics of movement in the presence of cam impingement. Methods: A 19-year-old male competitive footballer complained of sudden groin pain during a period of low athletic load. Biomechanical tests (3D posture and isometric strength analyses) showed that unbalanced individual strength training had resulted in an increased forward tilt of the pelvis. At the same time, cam impingement was confirmed radiologically, which obviously contributed to the sudden onset of the symptoms. The kicking technique of the athlete showed increased hip and trunk flexion, which also indicated a muscular imbalance. Targeted strength and stretching exercises three times a week improved the pelvic position in terms of reduced anteversion. At the same time, the patient performed strength exercises to improve his kicking technique. Results: After 8 weeks, improvements in his pelvic position and global posture and increased muscle strength could be verified. At the same time, the athlete was free of complaints again. Conclusions: When groin pain occurs in football players with cam impingement, special attention should be paid to the resting position of the pelvis in the sagittal plane. Correcting increased pelvic anteversion can prevent unfavourable end-grade collisions of the acetabulum and femoral head during kicking with strong hip flexion and adduction. Possible changes in the pelvic position due to adverse individual strength training performed by young athletes should always be kept in mind.
Head-Mounted Displays for Upper Limb Stroke Rehabilitation: A Scoping Review
Authors: Fregna Giulia , Paoluzzi Chiara , Baroni Andrea , Cano-de-la-Cuerda Roberto , Casile Antonino , Straudi Sofia
Published: November 2023

Upper extremity (UE) paresis is one of the most frequent and disabling clinical consequences after stroke. Head-Mounted Displays (HMDs) are wearable virtual reality devices that seem effective in promoting the recovery of functional abilities by increasing adherence levels in this population. This scoping review is aimed at collecting available evidence on the use of HMD-based immersive virtual reality systems for UE rehabilitation treatment in stroke survivors. Four electronic bibliographic databases were consulted from inception until 18 January 2023. A total of 19 clinical trials in which HMDs were used as a clinical tool for increasing UE functioning, as a single intervention or in adjunct to other rehab treatments, were included; no restrictions were applied for UE paresis severity or stroke onset. The large majority of the clinical trials involved chronic stroke patients (15 out of 19), with a wide range of UE impairments. Overall, HMD use seemed to be well-tolerated and promising for increasing UE motor function in adult chronic stroke survivors, with benefits in subjects’ arm use and independence. The possibility of executing highly realistic and task-oriented movements appears to be promising in enhancing gesture relevance, thus promoting new motor strategies in a “virtual ecological way”. Across studies, we found a high heterogeneity in protocol design and a lack of reporting that prevents us drawing conclusions regarding potential subgroups of patients that could benefit more from HMD-based interventions or suggested treatment modalities.
Neutrophil-to-Lymphocyte Ratio in Acute Exacerbation of Idiopathic Pulmonary Fibrosis
Authors: Arai Toru , Takimoto Takayuki , Takeuchi Naoko , Minomo Shojiro , Kagawa Tomoko , Inoue Yoshikazu
Published: November 2023

Background: This study aimed to clarify the prognostic value of the neutrophil-to-lymphocyte ratio (NLR) in patients with acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF). Methods: Eighty-six patients diagnosed with AE-IPF were included in this single-center retrospective study. The NLR was calculated by dividing the peripheral neutrophil count by the peripheral lymphocyte count. The cut-off values of the NLR for predicting 90-day survival were determined using receiver operating characteristic curve analysis. Oxygenation deterioration on days 4 and 8 relative to that on day 1 was clinically defined. The prognostic value of NLR was evaluated using Cox proportional hazard regression analysis. Results: The cut-off value of day-1, day-4, and day-8 NLRs for predicting 90-day survival was 12.13, 14.90, and 10.56, respectively. A higher day-1 NLR was a significant predictor of a poor prognosis in univariate and multivariate analyses. Survival was significantly better in patients without oxygenation deterioration on days 4 and 8 than in those with deterioration. Day-4 and day-8 NLR could predict 90-day survival in patients without oxygenation deterioration. Conclusions: Day-1 NLR was a useful predictor of 90-day survival in AE-IPF. Further, monitoring day-4 and day-8 NLRs and evaluating oxygenation deterioration may be useful for managing AE-IPF.
Computer-Assisted Intramedullary Nailing of Intertrochanteric Fractures Did Not Prevent Tip–Apex Distance Outliers
Authors: Hansen Rasmus Holm , Rölfing Jan Duedal , Nielsen Christian Lind , Brink Ole , Gundtoft Per Hviid
Published: November 2023

Intertrochanteric femoral fractures are commonly treated with intramedullary nails (IMNs). A tip–apex distance (TAD) of more than 20–25 mm is associated with an increased risk of cut-out. The Stryker Adaptive Positioning System (ADAPT) is a computer-assisted navigation system designed to reduce TADs. We aim to assess if the ADAPT reduces the number of outliers with a TAD > 20 mm. All patients with intertrochanteric fractures treated with an IMN between 1 September 2020 and 12 March 2022 were included. Patients were included in three periods: a pre-ADAPT period (55 patients); an ADAPT period (50 patients), where it was compulsory to use the system; and a post-ADAPT period after the discontinuation of the system (59 patients). The TADs and lag screw protrusions beyond the lateral cortex were measured. The median TADs in the three periods were 17.0 mm (8–31 mm), 15.5 mm (9–30 mm), and 18.0 mm (11–32 mm), respectively. The absolute number of outliers with a TAD > 20 mm decreased from 15/55 patients in the pre-ADAPT period to 11/50 patients during the ADAPT period. This observation was not statistically significant, but this is likely due to the lack of power of the present study to show changes of this magnitude. However, our expectation that the ADAPT would diminish outliers markedly or close to zero outliers was not met, as we observed 11/50 = 22% outliers with a TAD > 20 mm when using computer-assisted surgery, i.e., ADAPT and Gamma3 for intertrochanteric fractures. Based on these findings, the use of the ADAPT was discontinued at our level 1 trauma center.
Evaluation of Gelatin/Hyaluronic Acid-Generated Bridging in a 3D-Printed Titanium Cage for Bone Regeneration
Authors: Park Seong-Su , Farwa Ume , Hossain Mosharraf , Im Soobin , Lee Byong-Taek
Published: November 2023

3D-printed titanium (Ti) cages present an attractive alternative for addressing issues related to osteoporosis-induced fractures, accidental fractures, and spinal fusion surgery due to disc herniation. These Ti-based bone implants possess superior strength compared to other metals, allowing for versatile applications in orthopedic scenarios. However, when used as standalone solutions, certain considerations may arise, such as interaction with soft tissues. Therefore, to overcome these issues, the combination with hydrogel has been considered. In this study, to impart Ti with regenerative abilities a 3D-printed Ti cage was loaded with gelatin and hyaluronic acid (G-H) to improve the cell attachment ability of the Ti-based bone implants. The void spaces within the mesh structure of the 3D Ti cage were filled with G-H, creating a network of micro-sized pores. The filled G-H acted as the bridge for the cells to migrate toward the large inner pores of the 3D Ti cage. Due to the microporous surface and slow release of gelatin and hyaluronic acid, the biocompatibility of the coated Ti cage was increased with an elevation in osteoconduction as depicted by the up-regulation of bone-related gene expressions. The in vivo implantation in the rabbit femur model showed enhanced bone regeneration due to the coated G-H on the Ti cage compared to the pristine hollow Ti cage. The G-H filled the large holes of the 3D Ti cage that acted as a bridge for the cells to travel inside the implant and aided in the fast regeneration of bone.
YOLO-PAM: Parasite-Attention-Based Model for Efficient Malaria Detection
Authors: Zedda Luca , Loddo Andrea , Di Ruberto Cecilia
Published: November 2023

Malaria is a potentially fatal infectious disease caused by the Plasmodium parasite. The mortality rate can be significantly reduced if the condition is diagnosed and treated early. However, in many underdeveloped countries, the detection of malaria parasites from blood smears is still performed manually by experienced hematologists. This process is time-consuming and error-prone. In recent years, deep-learning-based object-detection methods have shown promising results in automating this task, which is critical to ensure diagnosis and treatment in the shortest possible time. In this paper, we propose a novel Transformer- and attention-based object-detection architecture designed to detect malaria parasites with high efficiency and precision, focusing on detecting several parasite sizes. The proposed method was tested on two public datasets, namely MP-IDB and IML. The evaluation results demonstrated a mean average precision exceeding 83.6% on distinct Plasmodium species within MP-IDB and reaching nearly 60% on IML. These findings underscore the effectiveness of our proposed architecture in automating malaria parasite detection, offering a potential breakthrough in expediting diagnosis and treatment processes.
Machine Learning-Based Image Processing for Ice Concentration during Chukchi and Beaufort Sea Trials
Authors: Kim Huichan , Park Sunho , Jeong Seong-Yeob
Published: November 2023

Growing interest in finding the optimal route through the arctic ocean, and sea ice concentration is also emerging as a factor to be considered. In this paper, an algorithm to calculate the sea ice concentration was developed based on the images taken during the Arctic voyage of the Korean icebreaker ARAON in July 2019. A sea ice concentration calculation program was developed using the image processing functions in open-source image processing library, called OpenCV. To develop the algorithm, parameter studies were conducted on red, green, blue (RGB) color space and hue, saturation, value (HSV) color space, and k-means clustering. To verify the algorithm for sea ice concentration calculation, it was applied to images taken during Araon’s Arctic voyages. Lens curvature and view point were corrected through camera calibration. To improve the accuracy of sea ice concentration calculation, a binarization model based on random forest was proposed. A parameter study for training image numbers and tree numbers was conducted to establish the random forest model. The calculated sea ice concentrations by random forest and k-means clustering were compared and discussed.
Solid-State Fermentation with White Rot Fungi (Pleurotus Species) Improves the Chemical Composition of Highland Barley Straw as a Ruminant Feed and Enhances In Vitro Rumen Digestibility
Authors: Wang Yuqiong , Gou Changlong , Chen Liming , Liao Yangci , Zhang Hang , Luo Lilong , Ji Jiahang , Qi Yu
Published: November 2023

Lignin degradation is important for enhancing the digestibility and improving the nutritive quality of ruminant feeds. White rot fungi are well known for their bioconversion of lignocellulosic biomass. The objective of this paper was to evaluate whether Lentinus sajor-caju, Pleurotus ostreatus, Phyllotopsis rhodophylla, Pleurotus djamor, Pleurotus eryngii, and Pleurotus citrinopileatus treatments altered the chemical compositions of highland barley straw constituents and enhanced their nutritional value as a ruminant feed. All white rot fungi significantly increased the relative crude protein (CP), ethyl ether extract (EE), starch, soluble protein (SP), and non-protein nitrogen (NPN) contents but decreased the ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and acid detergent insoluble protein (ADFIP) contents. In addition, L. sajor-caju treatment increased (p < 0.001) the levels of PA, PB2, PB3, CA, CB1, CB2, and CNSC, but reduced (p < 0.001) the PC and CC in the solid-state fermentation of highland barley straw. Maximum ligninlysis (50.19%) was optimally produced in the presence of 1.53% glucose and 2.29% urea at 22.72 ℃. The in vitro dry matter digestibility and total volatile fatty acid concentrations of fermented highland barley straw, as well as the fermentability, were optimized and improved with L. sajor-caju, which degraded the lignocellulose and improved the nutritional value of highland barley straw as a ruminant feed.