bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of the Mitochondrial Membrane Potential by the Cationic Dye JC-1 in L1210 Cells with Massive Overexpression of the Plasma Membrane ABCB1 Drug Transporter
Authors: Elefantova Katarina , Lakatos Boris , Kubickova Jana , Sulova Zdena , Breier Albert
Published: July 2018

JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient mitochondrial membrane potential (ΔΨ). The accumulation of JC-1 in these organelles leads to the formation J-aggregates (with a specific red fluorescence emission maximum at 590 nm), which is in addition to the typical green fluorescence of J-monomers (emission maximum of ∼529 nm). The lack of mitochondrial ΔΨ leads to the depression of JC-1 mitochondrial accumulation and a decrease in J-aggregate formation. Therefore, the ratio between the red and green fluorescence of cells loaded with JC-1 is often used for the detection of the mitochondrial membrane potential. However, JC-1 represents a suitable substrate of the multidrug transporter P-glycoprotein (P-gp). Therefore, the depression of the JC-1 content in intracellular space and particularly in the mitochondria to a level that is inefficient for J-aggregate formation could be expected in P-gp-positive cells. In the current paper, we proved this behavior on parental P-gp-negative L1210 (S) cells and their P-gp-positive variants obtained by either selection with vincristine (R) or transfection with the human gene encoding P-gp (T). P-glycoprotein inhibitors cyclosporine A and verapamil fail to restore JC-1 loading of the R and T cells to an extent similar to that observed in S cells. In contrast, the noncompetitive high affinity P-gp inhibitor tariquidar fully restored JC-1 accumulation and the presence of the typical red fluorescence of J-aggregates. In the presence of tariquidar, measurement of the JC-1 fluorescence revealed similar levels of mitochondrial membrane potential in P-gp-negative (S) and P-gp-positive cells (R and T).
Impact of Drosophila Models in the Study and Treatment of Friedreich’s Ataxia
Authors: Monnier Véronique , Llorens Jose Vicente , Navarro Juan Antonio
Published: July 2018

Drosophila melanogaster has been for over a century the model of choice of several neurobiologists to decipher the formation and development of the nervous system as well as to mirror the pathophysiological conditions of many human neurodegenerative diseases. The rare disease Friedreich’s ataxia (FRDA) is not an exception. Since the isolation of the responsible gene more than two decades ago, the analysis of the fly orthologue has proven to be an excellent avenue to understand the development and progression of the disease, to unravel pivotal mechanisms underpinning the pathology and to identify genes and molecules that might well be either disease biomarkers or promising targets for therapeutic interventions. In this review, we aim to summarize the collection of findings provided by the Drosophila models but also to go one step beyond and propose the implications of these discoveries for the study and cure of this disorder. We will present the physiological, cellular and molecular phenotypes described in the fly, highlighting those that have given insight into the pathology and we will show how the ability of Drosophila to perform genetic and pharmacological screens has provided valuable information that is not easily within reach of other cellular or mammalian models.
Simulated Microgravity Reduces Focal Adhesions and Alters Cytoskeleton and Nuclear Positioning Leading to Enhanced Apoptosis via Suppressing FAK/RhoA-Mediated mTORC1/NF-κB and ERK1/2 Pathways
Authors: Zhao Tuo , Li Rong , Tan Xin , Zhang Jun , Fan Cuihong , Zhao Qin , Deng Yulin , Xu Aizhang , Lukong Kiven Erique , Genth Harald , Xiang Jim
Published: July 2018

Simulated-microgravity (SMG) promotes cell-apoptosis. We demonstrated that SMG inhibited cell proliferation/metastasis via FAK/RhoA-regulated mTORC1 pathway. Since mTORC1, NF-κB, and ERK1/2 signaling are important in cell apoptosis, we examined whether SMG-enhanced apoptosis is regulated via these signals controlled by FAK/RhoA in BL6-10 melanoma cells under clinostat-modelled SMG-condition. We show that SMG promotes cell-apoptosis, alters cytoskeleton, reduces focal adhesions (FAs), and suppresses FAK/RhoA signaling. SMG down-regulates expression of mTORC1-related Raptor, pS6K, pEIF4E, pNF-κB, and pNF-κB-regulated Bcl2, and induces relocalization of pNF-κB from the nucleus to the cytoplasm. In addition, SMG also inhibits expression of nuclear envelope proteins (NEPs) lamin-A, emerin, sun1, and nesprin-3, which control nuclear positioning, and suppresses nuclear positioning-regulated pERK1/2 signaling. Moreover, rapamycin, the mTORC1 inhibitor, also enhances apoptosis in cells under 1 g condition via suppressing the mTORC1/NF-κB pathway. Furthermore, the FAK/RhoA activator, toxin cytotoxic necrotizing factor-1 (CNF1), reduces cell apoptosis, restores the cytoskeleton, FAs, NEPs, and nuclear positioning, and converts all of the above SMG-induced changes in molecular signaling in cells under SMG. Therefore, our data demonstrate that SMG reduces FAs and alters the cytoskeleton and nuclear positioning, leading to enhanced cell apoptosis via suppressing the FAK/RhoA-regulated mTORC1/NF-κB and ERK1/2 pathways. The FAK/RhoA regulatory network may, thus, become a new target for the development of novel therapeutics for humans under spaceflight conditions with stressed physiological challenges, and for other human diseases.
The Anti-Wrinkle Mechanism of Melatonin in UVB Treated HaCaT Keratinocytes and Hairless Mice via Inhibition of ROS and Sonic Hedgehog Mediated Inflammatory Proteins
Authors: Park Eun Kyung , Lee Hyo-Jung , Lee Hyemin , Kim Ju-Ha , Hwang Jisung , Koo Ja Il , Kim Sung-Hoon
Published: July 2018

Though melatonin is known to improve ultraviolet B (UVB)-induced oxidative damage and inflammatory conditions via the blockade of the nuclear factor (NF)-κB, interleukin (IL)-6, there is no report on the anti-wrinkle effect of melatonin to date. Hence in the present study, the anti-wrinkle mechanism of melatonin was elucidated in UVB treated HaCaT keratinocytes and hairless mice. Herein melatonin protected against a radical initiator tert-Butyl hydroperoxide (t-BOOH) induced reactive oxygen species (ROS) production, matrix metalloprotease 1 (MMP-1), pro-collagen and cytotoxicity in HaCaT keratinocytes. Additionally, melatonin suppressed the expression of sonic hedgehog (SHH) and GLI1 for hedgehog signaling and p-NF-κB, cyclooxygenase (COX-2), phospho-extracellular signal-regulated kinase-1 (p-ERK) for inflammatory responses in UVB treated HaCaT keratinocytes. Furthermore, melatonin protected skin from wrinkle formation, transdermal water loss in hairless mice irradiated by UVB for 8 weeks. Notably, melatonin prevented against epidermal thickness and dermal collagen degradation in UVB irradiated hairless mice by Hematoxylin and Eosin and Masson’s trichrome staining. Taken together, these findings suggest that melatonin reduces wrinkle formation via inhibition of ROS/SHH and inflammatory proteins such as NF-κB/COX-2/ERK/MMP1.
Comparative Proteomic Profiling between Each of Two Consecutive Developmental Stages of the Solanum Fruit Fly, Bactrocera latifrons (Hendel)
Authors: Chang Chiou Ling , Geib Scott M.
Published: July 2018

The Solanum fruit fly, Bactrocera latifrons (Hendel), has a complex life cycle including multiple stages (egg, larva, pupa, and adult). Understanding the details of “what”, “when”, “where”, “why”, and “how” many hundred thousand proteins operate in this insect, interact, and express between each two consecutive developmental stages at molecular level not only can expand our knowledge, but also lead to the development of novel fruit fly control techniques. We tried to find what, when, and where in this study. Why and how will be presented in upcoming papers. We conducted a proteome profiling using 2-D gel electrophoresis and mass spectrometry. Samples of 3-day-old eggs, 1- and 10-day-old larvae, 1- and 10-day-old pupae, 1- and 9-day-old females and males of B. latifrons were used. A custom peptide database, derived from the de novo B. latifrons whole genome assembly was used for peptide identification. Differentially expressed proteins (DEPs) with significant fold expression and protein functions between two consecutive developmental stages were identified, annotated, described, and listed in gel images and/or charts. With this foundational information, we are not only providing valuable information, but also any impacts due to the biotic or abiotic environmental factors can be identified and manipulated, and lead to further research on gene editing and biomarker discovery.
Vitamin D’s Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism
Authors: De Luca Paola , De Girolamo Laura , Perucca Orfei Carlotta , Viganò Marco , Cecchinato Riccardo , Brayda-Bruno Marco , Colombini Alessandra
Published: July 2018

Vitamin D is known to have immunomodulatory effects, is involved in osteo-cartilaginous metabolism, and may have a role in human intervertebral disc pathophysiology. Although a link between vitamin D receptor (VDR) gene variants and disc degeneration-related pathologies has been observed, its functional contribution to pathologic processes has not been assessed yet. The aim of this study was to investigate the response of disc cells to vitamin D in terms of the regulation of proliferation, metabolism, and inflammatory processes, with a particular focus on the FokI VDR genotype. However, although it was found that vitamin D had a pro-apoptotic effect regardless of genotype, an up-regulation of IL-1Ra and downregulation of IL-6 was found to be evident only in Ff cells. Regarding the metabolic effects, in Ff cells, vitamin D promoted an upregulation of the aggrecan in inflammatory conditions but did not have an effect on the expression of collagen-related markers. Moreover, cells bearing the Ff genotype were the most responsive to vitamin D in the upregulation of catabolic markers. In addition, in contrast to the FF genotype, vitamin D downregulated the vitamin D-dependent signaling pathway in inflamed Ff cells, counteracting the inflammation-mediated catabolic effects. In conclusion, Ff cells were found to be more responsive to the anti-inflammatory and catabolic effects of vitamin D, which is likely to be related to matrix remodeling.
A Novel Monoclonal Antibody Targets Mucin1 and Attenuates Growth in Pancreatic Cancer Model
Authors: Wu Guang , Maharjan Sony , Kim Dongbum , Kim Jung Nam , Park Byoung Kwon , Koh Heeju , Moon Kyungduk , Lee Younghee , Kwon Hyung-Joo
Published: July 2018

Mucin1 (MUC1) is a highly glycosylated transmembrane protein that plays a crucial role in the lubrication and protection of normal epithelial cells. However, MUC1 has emerged as a potential target for cancer therapy because it is overexpressed and functions in several types of cancers. Recently, we produced a monoclonal antibody (the anti-hMUC1 antibody) specific to the extracellular region of the MUC1 subunit MUC1-C to evaluate the utility of using anti-MUC1 antibodies in pancreatic cancer models. The anti-hMUC1 antibody recognized the MUC1-C protein in pancreatic cancer cells. Based on immunostaining and confocal image analyses, the anti-hMUC1 antibody initially bound to the cell membrane then was internalized in cancer cells that express MUC1. The anti-hMUC1 antibody suppressed epidermal growth factor (EGF)-mediated extracellular signal–regulated kinase (ERK) phosphorylation and cyclin D1 expression. When the anti-hMUC1 antibody was injected into a xenograft mouse model and traced using an in vivo imaging system, we observed that the anti-hMUC1 antibody was localized to MUC1-expressing pancreatic tumors. Importantly, the anti-hMUC1 monoclonal antibody suppressed pancreatic tumor growth in mice. According to immunohistochemistry analysis using a pancreatic cancer tissue array and the anti-hMUC1 antibody, MUC1 was highly expressed in human pancreatic cancer tissues compared to normal tissues. Therefore, we conclude that the anti-hMUC1 antibody specifically targets MUC1 and suppresses its function in pancreatic cancer in vitro and in vivo and can be further developed as a promising targeted therapy to treat pancreatic cancer.
DEAD-Box Protein RNA-Helicase DDX6 Regulates the Expression of HER2 and FGFR2 at the Post-Transcriptional Step in Gastric Cancer Cells
Authors: Tajirika Toshihiro , Tokumaru Yoshihisa , Taniguchi Kohei , Sugito Nobuhiko , Matsuhashi Nobuhisa , Futamura Manabu , Yanagihara Kazuyoshi , Akao Yukihiro , Yoshida Kazuhiro
Published: July 2018

The human DEAD/H-box RNA helicase DDX6 (RCK/p54) is a protein encoded by the fusion gene from the t(11;14)(q23;q32) chromosomal translocation observed in human B-cell lymphoma cell line RC-K8. DDX6 has a variety of functions such as translation initiation, pre-mRNA splicing, and ribosome assembly. However, details of the regulatory mechanism governing DDX6 and the functions of DDX6 are largely unknown. Previously, we reported that DDX6 is overexpressed in most malignant cell lines and clinical colorectal tumor samples and that DDX6 positively contributes to the pathogenesis of various cancers. In the current study, we aimed at revealing the function of DDX6 in HER2 and FGFR2 related human gastric cancer (GC) by using clinical samples and GC cell lines. DDX6 protein was overexpressed in about 60% of the clinical samples; HER2, in 35%; and FGFR2, in 30%, (n = 20). Interestingly, the DDX6 protein was overexpressed in all HER2-positive samples (n = 7), and in 83% (5 of 6) of the FGFR2-positive samples, which could reflect the contribution of DDX6 to the expression of HER2 and FGFR2. In the GC cell line MKN7, which has HER2 amplification, the knockdown of DDX6 by siR-DDX6 led to the decreased expression of the HER2 protein. On the other hand, the knockdown of HER2 did not influence the DDX6 expression. Similar results were also obtained for the KATO-III and HSC39 cell lines having amplified FGFR2 expression. The increased expression of DDX6 induced a significantly increased expression of the HER2 protein without increasing the mRNA expression. The results of an RNP Immunoprecipitation (RIP)-assay using GC cells indicated that the DDX6 protein acted as an RNA-binding protein for HER2 and FGFR2 mRNAs and positively regulated their post-transcriptional processes. These findings demonstrated that DDX6 was an upstream molecule that positively regulated the expression of HER2 and FGFR2 at the post-transcriptional step in GC cells.
Excellence in Turbomachinery Research: The Best of the 12th European Turbomachinery Conference
Authors: Manna Marcello
Published: July 2018

n/a
An Improved Genetic Algorithm with a New Initialization Mechanism Based on Regression Techniques
Authors: Hassanat Ahmad B. , Prasath V. B. Surya , Abbadi Mohammed Ali , Abu-Qdari Salam Amer , Faris Hossam
Published: July 2018

Genetic algorithm (GA) is one of the well-known techniques from the area of evolutionary computation that plays a significant role in obtaining meaningful solutions to complex problems with large search space. GAs involve three fundamental operations after creating an initial population, namely selection, crossover, and mutation. The first task in GAs is to create an appropriate initial population. Traditionally GAs with randomly selected population is widely used as it is simple and efficient; however, the generated population may contain poor fitness. Low quality or poor fitness of individuals may lead to take long time to converge to an optimal (or near-optimal) solution. Therefore, the fitness or quality of initial population of individuals plays a significant role in determining an optimal or near-optimal solution. In this work, we propose a new method for the initial population seeding based on linear regression analysis of the problem tackled by the GA; in this paper, the traveling salesman problem (TSP). The proposed Regression-based technique divides a given large scale TSP problem into smaller sub-problems. This is done using the regression line and its perpendicular line, which allow for clustering the cities into four sub-problems repeatedly, the location of each city determines which category/cluster the city belongs to, the algorithm works repeatedly until the size of the subproblem becomes very small, four cities or less for instance, these cities are more likely neighboring each other, so connecting them to each other creates a somehow good solution to start with, this solution is mutated several times to form the initial population. We analyze the performance of the GA when using traditional population seeding techniques, such as the random and nearest neighbors, along with the proposed regression-based technique. The experiments are carried out using some of the well-known TSP instances obtained from the TSPLIB, which is the standard library for TSP problems. Quantitative analysis is carried out using the statistical test tools: analysis of variance (ANOVA), Duncan multiple range test (DMRT), and least significant difference (LSD). The experimental results show that the performance of the GA that uses the proposed regression-based technique for population seeding outperforms other GAs that uses traditional population seeding techniques such as the random and the nearest neighbor based techniques in terms of error rate, and average convergence.
Aerial-Image Denoising Based on Convolutional Neural Network with Multi-Scale Residual Learning Approach
Authors: Chen Chong , Xu Zengbo
Published: July 2018

Aerial images are subject to various types of noise, which restricts the recognition and analysis of images, target monitoring, and search services. At present, deep learning is successful in image recognition. However, traditional convolutional neural networks (CNNs) extract the main features of an image to predict directly and are limited by the requirements of the training sample size (i.e., a small data size is not successful enough). In this paper, using a small sample size, we propose an aerial-image denoising recognition model based on CNNs with a multi-scale residual learning approach. The proposed model has the following three advantages: (1) Instead of directly learning latent clean images, the proposed model learns the noise from noisy images and then subtracts the learned residual from the noisy images to obtain reconstructed (denoised) images; (2) The developed image denoising recognition model is beneficial to small training datasets; (3) We use multi-scale residual learning as a learning approach, and dropout is introduced into the model architecture to force the network to learn to generalize well enough. Our experimental results on aerial-image denoising recognition reveal that the proposed approach is highly superior to the other state-of-the-art methods.
Synthesis, Structural, and Magnetic Characterization of a Mixed 3d/4f 12-Metallacrown-4 Family of Complexes
Authors: Athanasopoulou Angeliki A. , Carrella Luca M. , Rentschler Eva
Published: July 2018

A new family of complexes (tBu4N){[LnIII(O2CBut)4][12-MC-Mn(III)N(shi)-4]}·5CH2Cl2 (Ln = Gd (1) and Tb (2)), (tBu4N)2{[YIII(O2CBut)4][12-MC-Mn(III)N(shi)-4]}(ClO4) (3); where shiH3 = salicylhydroxamic acid; ButCO2− = pivalate ions; tBu4N = tetrabutylammonium and ClO4− = perchlorate ions, has been isolated. The reaction of salicylhydroxamic acid with Mn(O2CBut)2·2H2O, Ln(NO3)3·xH2O, tBu4NClO4 in the presence of morpholine (C4H9NO) led to the isolation of compounds 1–3. The complexes belong to the 12-MC-4 family of Metallacrowns (MCs) possessing a central {Mn4IIILnIII(µ-NO)4}11+ core with the four MnIII atoms occupying the periphery positions, while the formed [Mn–N–O] repeating unit, assists in the accommodation of the LnIII atom in the center of the ring. Peripheral ligation is provided by four η1:η1:μ pivalate ions. Direct current magnetic susceptibility (dc) measurements revealed the presence of predominant antiferromagnetic exchange interactions within the metal centers. A 1-J fitting model was used in order to quantify the MnIII–MnIII interactions and fitting of the data, for the diamagnetic YIII analogue, gave J = −3.74 cm−1 and gMn(III) = 2.07. Fitting of the {Mn4Gd} compound using a 2-J model, counting additionally for the MnIII–GdIII interactions, revealed values of J1 = −3.52 cm−1, J2 = −0.45 cm−1, and gMn(III) = 1.99.
Monitoring and Biosurveillance Tools for the Brown Marmorated Stink Bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae)
Authors: Acebes-Doria Angelita L. , Morrison William R. , Short Brent D. , Rice Kevin B. , Bush Hayley G. , Kuhar Thomas P. , Duthie Catherine , Leskey Tracy C.
Published: July 2018

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive pest of numerous agricultural crops with an increasing global distribution. Finding simple and reliable monitoring tools for H. halys agricultural and surveillance programs is imperative. In 2016, we compared standard pyramid traps to clear sticky cards attached atop wooden stakes and evaluated two commercially formulated lures (Trécé and AgBio) with low and high rates of the H. halys aggregation pheromone (PHER) and pheromone synergist (MDT) at 12 sites (low: 5 mg PHER + 50 mg MDT; high: 20 mg PHER + 200 mg MDT). In 2017, we reevaluated lure efficacy using only the clear sticky traps at six locations. Sites were classified as having low, moderate, or high relative population densities of H. halys in 2016, and as very low or low densities of H. halys in 2017. Although clear sticky traps captured fewer adults and nymphs than pyramid traps, their captures were generally correlated at all population levels indicating that clear sticky traps can reliably monitor H. halys presence and relative abundance regardless of relative population density. During both years, adult and nymphal captures were significantly greater in traps baited with Trécé lures than with AgBio lures. Captures were greater in traps baited with high loading rate lures for each lure type, and with the exception of traps baited with AgBio lures at high relative density sites in 2016, H. halys captures in traps with low and high loading rates of each lure type were correlated for both years. Comparison of yellow and clear sticky cards indicated they performed equally, but yellow cards captured more nontargets. In summary, clear sticky traps attached atop wooden posts and baited with H. halys pheromone and pheromone synergist lures are an effective option for this pest monitoring and detection.
A Survey on the Knowledge and Attitudes of Italian Medical Students toward Body Donation: Ethical and Scientific Considerations
Authors: Ciliberti Rosagemma , Gulino Matteo , Gazzaniga Valentina , Gallo Fabio , Vellone Valerio , De Stefano Francesco , Santi Pierluigi , Baldelli Ilaria
Published: July 2018

Post mortem body donation (PMBD) for medical training and research plays a key role in medical-surgical education. The aim of this study is to evaluate Italian medical students’ awareness and attitudes regarding this practice. A questionnaire was sent to 1781 Italian medical students (MS). A total of 472 MS responded: 406 (92.91%) had a strongly positive attitude to PMBD, while 31 (7.09%) were not in favor. The majority of subjects were Catholic (56.36%), while 185 and 21 subjects, said that they did not hold any religious beliefs, or were of other religions, respectively. Multivariate analysis showed significant associations (p-values < 0.05) between PMBD and religion, as well as perceptions of PMBD as an act of altruism, a tool for learning surgical practices, body mutilation, and an act contrary to faith. Although Italian MS believed cadaver dissection to be an important part of their education, they did not know much about it and had not received training on this altruistic choice. As future doctors, MS can play an important role in raising public awareness of the importance of PMBD for medical education and research. Specific educational programs to improve knowledge of this topic among MS are needed.
Assessment of Cutting Performance of Cemented Tungsten Carbide Drills in Drilling Multidirectional T700 CFRP Plate
Authors: Ali Mohsin , Xiang Li , Yue Dong , Liu Guojian
Published: July 2018

The drilling of carbon fiber reinforced polymer (CFRP) plate is very critical in its structural applications in aeronautical, aerospace, and automobile industries. For ensuring the good quality of drilled holes in terms of least drilling damage and prolonged tool life, suitable selection of drill geometry and material, and drilling parameters—such as cutting speed and feed rate—are imperative in the drilling of CFRP plate. This study aims to evaluate the cutting performance of conventional two-flute twist drills made of cemented tungsten carbide YG6X (WC-6 wt % Co) for the dry drilling of the multidirectional T700 CFRP plate. The effects of varying cutting speed and tool wear pattern on the drilling performance are analyzed. The drilling performance is assessed in terms of hole quality by the qualitative and quantitative analysis of drilling-induced delamination and hole diameter. Furthermore, the correlation between the tool wear progression and the drilled hole quality is established. Through this assessment, a suitable set of drilling parameters, i.e., cutting speed of 9000 rpm and feed rate of 400 mm/min, is proposed for producing the best quality holes for multidirectional T700 CFRP plate.
Treatment of Infections Due to Aspergillus terreus Species Complex
Authors: Lass-Flörl Cornelia
Published: July 2018

The Aspergillus terreus species complex is found in a wide variety of habitats, and the spectrum of diseases caused covers allergic bronchopulmonary aspergillosis, Aspergillus bronchitis and/or tracheobronchitis, and invasive and disseminated aspergillosis. Invasive infections are a significant cause of morbidity and mortality mainly in patients with hematological malignancy. The section Terrei covers a total of 16 accepted species of which most are amphotericin B resistant. Triazoles are the preferred agents for treatment and prevention of invasive aspergillosis. Poor prognosis in patients with invasive A. terreus infections seems to be independent of anti-Aspergillus azole-based treatment.
Ultra-Low Friction on Tetrahedral Amorphous Diamond-Like Carbon (ta-C) Lubricated with Ethylene Glycol
Authors: Bachmann Svenja , Schulze Marcus , Krell Lisa , Merz Rolf , Wahl Michael , Stark Robert
Published: July 2018

Lubricated tetrahedral amorphous carbon coatings can show a very complex tribological behavior. In particular, friction regimes with extremely low friction have been observed. In tribological experiments with a ta-C/steel friction pair that was lubricated with ethylene glycol, we observed a sudden and very strong decrease in the effective friction coefficient from 0.45 to 0.01 after running-in. By varying different components of the tribological system after this abrupt decrease we investigated the role of the counter-body, the lubricant and the coating. To investigate the surface chemistry, static time-of-flight secondary ion mass spectrometry (ToF-SIMS), dynamic secondary ion mass spectrometry (SIMS), and X-ray photoelectron spectroscopy (XPS) measurements were performed. Using deuterated lubricants, ToF-SIMS measurements allowed us to distinguish adsorption of hydrogen and hydroxyl-groups from the lubricant from the adsorption from the environment. Deuterated hydroxyl-groups from the lubricant adsorbed to the surface during the experiment. In particular, more adsorbed deuterated hydroxyl-groups were detected prior to the sudden decrease in the friction coefficient. Thus, the sudden decrease in the coefficient of friction was most likely caused by an interplay between the lubricant, the ta-C coating and the counter-body which lead to the formation of transfer and adsorption layers.
1-(4-Hexylbenzoyl)-3-methylthiourea
Authors: Ruswanto Ruswanto , Mardianingrum Richa , Lestari Tresna , Nofianti Tita , Siswandono Siswandono
Published: July 2018

The 1-(4-hexylbenzoyl)-3-methylthiourea compound has been successfully synthesized by reacting 4-hexylbenzoyl chloride and 1-methylthiourea via the reflux method using a triethylamine catalyst. The 1-(4-hexylbenzoyl)-3-methylthiourea compound was identified by UV-visible, FT-IR, 13C/1H-NMR and Mass spectrophotometry. From the activity test on four cancer cell lines (HeLa, T47D, WiDr and MCF7 cell), it could be seen that it had better activity on four cancer cells than the control, hydroxyurea.
Environmentally-Friendly Extraction of Cellulose Nanofibers from Steam-Explosion Pretreated Sugar Beet Pulp
Authors: Yang Wengang , Feng Yanhong , He Hezhi , Yang Zhitao
Published: July 2018

Cellulose nanofibers (CNFs) with an average diameter of 22 nm were prepared from sugar beet pulp (SBP) via an environmentally-friendly method. Steam-explosion pretreated SBP was treated with hydrogen peroxide (H2O2) bleaching, high-speed blending, and ultrasonic treatment. Thermogravimetric analysis showed that hemicellulose was partially hydrolyzed in the steam-cooking stage, pectin was removed in the explosion stage, and lignin was removed by H2O2 bleaching. The removal of non-cellulosic components was confirmed by Fourier-transform infrared (FT-IR) spectroscopy. Morphological analysis showed that steam-explosion pretreatment largely extracted the binder materials of hemicellulose and pectin. This exposed the microfibrillated cellulosic fibers, which promoted subsequent nanofibrillation. X-ray diffraction showed that the CNFs had a crystallinity index of 62.3%. The CNFs had good thermal stability, and thus have potential for use as fillers in polymer matrices. The only chemical reagent used in this green method was H2O2. Combining H2O2 bleaching with steam explosion, high-speed blending, and ultrasonic treatment reduced the overall energy consumption and increased the efficiency of the CNFs extraction. The method, therefore, has potential application in industrial processes.
Enhancement of Dynamic Damping in Eco-Friendly Railway Concrete Sleepers Using Waste-Tyre Crumb Rubber
Authors: Kaewunruen Sakdirat , Li Dan , Chen Yu , Xiang Zhechun
Published: July 2018

There is no doubt that the use of waste rubber in concrete applications is a genius alternative because Styrene is the main component of rubber, which has a strong toxicity and is harmful to humans. Therefore, it will significantly reduce impacts on the environment when waste rubber can be recycled for genuine uses. In this paper, the dynamic properties of high-strength rubberised concrete have been investigated by carrying out various experiments to retain the compressive strength, tensile strength, flexural strength, electrical resistivity, and damping characteristics by replacing fine aggregates with micro-scale crumb rubber. Over 20 variations of concrete mixes have been performed. The experimental results confirm that a decrease in the compressive strength can be expected when the rubber content is increased. The new findings demonstrate that the high-strength concrete can be enhanced by optimal rubber particles in order to improve splitting tensile and flexural strengths, damping properties, and electrical resistivity. It is therefore recommended to consider the use of rubberised concrete (up to 10 wt. % crumb rubber) in designing railway sleepers as this will improve the service life of railway track systems and reduce wastes to the environment.
Four New C9 Metabolites from the Sponge-Associated Fungus Gliomastix sp. ZSDS1-F7-2
Authors: Zhang Jun , Yang Zhiqiang , Liang Yan , Zhong Liping , Lin Huiting , Zhong Balian , Li Liangchun , Xu Shihai , Liu Yonghong
Published: July 2018

Four new structurally related metabolites, one γ-lactone named gliomasolide F (1), one δ-lactone named gliomasolide G (2), and two medium-chain fatty acids named gliomacids A–B (3–4), each containing nine carbons in total, were identified from the sponge-associated fungus Gliomastix sp. ZSDS1-F7-2. The planar chemical structures of these novel C9 metabolites were elucidated by nuclear magnetic resonance (NMR) spectroscopic methods, in connection with the analysis of high-resolution mass spectrometry (HRMS) and infrared (IR) data. The absolute configuration of 1, was determined by comparisons of experimental circular dichroism (CD) and optical rotation (OR) value with corresponding ones computed by quantum chemistry. The relative configuration of 2 was determined by the Nuclear Overhauser effect spectroscopy (NOESY) spectrum, while its absolute configuration was tentatively determined in view of the biogenetic and biosynthetic relationships between 1 and 2. Compounds 3–4, originally as an inseparable mixture, were successfully isolated after chemical modifications. The stereo-chemistries of compounds 3–4 were assumed by comparison of 13C NMR with those of the similar moiety reported in literature, in addition to the biogenetic and biosynthetic relationships with 1. The plausible biosynthetic relationships among these four C9 metabolites were supposed. Biologically, compounds 1–4 showed no cytotoxic effect against HeLa cell line at concentrations up to 25 μg/mL, while 1 exhibited moderate antifouling activity against the settlement of Balanus amphitrite larvae with IC50 being 12.8 μg/mL and LC50 > 25 μg/mL. The co-occurrence of macrolides gliomasolides A—E and four C9 metabolites in the same fermentation culture made us assume that these C9 metabolites might be biosynthetic building blocks toward the construction of more complex macrolides such as gliomasolides A—E or other unidentified polyketides.
Marine Carbohydrate-Based Compounds with Medicinal Properties
Authors: Vasconcelos Ariana A. , Pomin Vitor H.
Published: July 2018

The oceans harbor a great diversity of organisms, and have been recognized as an important source of new compounds with nutritional and therapeutic potential. Among these compounds, carbohydrate-based compounds are of particular interest because they exhibit numerous biological functions associated with their chemical diversity. This gives rise to new substances for the development of bioactive products. Many are the known applications of substances with glycosidic domains obtained from marine species. This review covers the structural properties and the current findings on the antioxidant, anti-inflammatory, anticoagulant, antitumor and antimicrobial activities of medium and high molecular-weight carbohydrates or glycosylated compounds extracted from various marine organisms.
Microbial infection and its control in cases of symptomatic apical periodontitis: A review
Authors: Skučaitė Neringa , Pečiulienė Vytautė , Mačiulskienė Vita
Published: July 2018

Apical periodontitis is an inflammation of dental periapical tissues developed as a response to colonization of microorganisms in root canal system. Etiology of periapical pathology is associated with different species of microorganisms that are not fully defined yet. The changes in the composition of root canal microbiota as well as other factors, such as host resistance to various infections and concomitant viral infection, etc., can influence development of the symptomatic apical periodontitis. Etiology of disease is reviewed in this article. The purpose of treatment of symptomatic apical periodontitis is to eliminate the infection in root canal system and to obtain relief of symptoms. It can be done by conventional root canal therapy, which can be combined with anti-inflammatory medication. Indications for antibiotic therapy in such cases are limited to particular occasions, which are considered in article. Nevertheless, findings show that usually dentists prescribe antibiotics improperly in clinical practice. It can render drugs ineffective against diseases of dental origin as well as against potentially fatal infectious diseases. Selection of antibiotics for the treatment of root canal infections is reviewed in this article. Importance of antimicrobial susceptibility testing is emphasized. There is a need for more research on microbial causes and interactions in different forms of apical periodontitis to improve diagnosis and treatment.
Assessment of urinary incontinence in the elderly using the InterRAI-AC instrument
Authors: Kučikienė Odeta , Lesauskaitė Vita , Macijauskienė Jūratė , Jievaltienė Goda
Published: July 2018

The aim of this study was to identify and evaluate the prevalence of urinary incontinence and risk factors that influenced it among inpatients treated in the departments of internal medicine. Material and methods. A total of 151 inpatients were questioned using a standardized geriatric questionnaire (InterRAI-Acute Care). Inpatients aged 65 years and more and who gave written informed consent were enrolled into the study. The mean age of the inpatients was 78±0.6 years. There were 58.9% of women and 41.06% of men. Results. Urinary incontinence was significantly influenced by the age of the inpatients. Inpatients with urinary incontinence were 3 years older on the average as compared to those without urinary continence (P<0.025). Women were more frequently affected than men (74.2% vs. 48.4%). A significant association between urinary and fecal incontinence and memory problems, movement disorders, delirium, dependence in daily activities, falls was established. The odds of having double incontinence were increased by dementia (OR=20.9; 95%, CI 2.3–186) and residual effects of a stroke (OR=3.5; 95%, CI 1.2–9.6). The prevalence of urinary incontinence decreased from 63.6% before hospitalization to 39.7% after hospitalization. According to standard medical documentation, urinary incontinence was diagnosed in 3.3% of cases, while using the interRAI-AC questionnaire, it was documented in 63.6% of cases. Conclusions. The prevalence of urinary incontinence increases in the elderly; therefore, it has to be investigated and treated. Memory problems, delirium, dependence in daily activities, movement disorders, and falls are directly related to the risk of urinary, fecal, and double incontinence among elderly inpatients. Double incontinence was significantly influenced by dementia (20.9 times) and residual effects of a stroke (3.5 times). Underdiagnosis of urinary and fecal incontinence in inpatients burdens the possibility of providing aid for elderly patients with this disorder.
Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro
Authors: Labanauskienė Jūratė , Šatkauskas Saulius , Kirvelienė Vida , Venslauskas Mindaugas , Atkočius Vydmantas , Didžiapetrienė Janina
Published: July 2018

The aim of our study was to determine if electroporation could improve the efficacy of photodynamic tumor therapy. A disadvantage of photodynamic therapy is a slow and in some cases insufficient accumulation of photosensitizer in tumor tissue, which could restrict the achievement of an efficient dose. Under the action of electric pulses, cells undergo membrane electroporation, which results in an increased permeability to various exogenous molecules. In this study, murine hepatoma MH22A cells were exposed to light in vitro in the presence of a photosensitizer, either chlorin e6 or aluminum phthalocyanine tetrasulfonate, following electroporation. Accumulation of the photosensitizers was registered by fluorescence microscopy. Cell viability was determined by the MTT assay. Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers.
Changes of vision and refraction in patients with thyroid pathology
Authors: Jankauskienė Jūratė , Jakštaitė Vaida , Smalinskas Vilmantas
Published: July 2018

Objective. The aim of the study was to assess the relationship between thyroid pathology and visual acuity, including refraction disorders. Material and methods. The data of visual acuity, refraction, and exophthalmos were analyzed, which were obtained from 105 patients with thyroid pathology. The data were compared with the findings of 23 healthy persons of similar age (control group). Results. In case of thyroid pathology, visual acuity (right eye 0.71±0.035, left eye 0.73±0.045) was worse than in the control group (right eye 0.89±0.06, P<0.05; left eye 0.92±0.07, P<0.05). Myopia dominated in patients with thyroid pathology (refraction: right eye M 1.1±0.61 D, left eye M 1.0±0.64 D). In patients with exophthalmos of ≥17 mm, visual acuity was significantly worse (right eye 0.68±0.04, left eye 0.68±0.03) than in persons of the control group (right eye 0.89±0.06, P<0.05; left eye 0.92±0.07, P<0.05), and myopic refraction was found more frequently (right eye M 1.3±0.48 D, left eye M 1.3±0.56 D). Visual acuity was significantly better in patients when exophthalmos was <17 mm (right eye 0.80±0.05, P<0.05; left eye 0.80±0.05, P<0.05) than in cases when exophthalmos was ≥17 mm. Conclusions. Thyroid pathology and exophthalmos have an influence on visual acuity and refraction. In case of thyroid pathology, visual acuity appears to be worse than in the control group, and myopic refraction is more frequent. Visual acuity was found to be worse in patients with exophthalmos being ≥17 mm than in the control group, and myopic refraction was found to be more frequent.
Impact of duration and treatment of arterial hypertension on health-related quality of life
Authors: Raškelienė Vilma , Babarskienė Marija , Macijauskienė Jūratė , Šeškevičius Arvydas
Published: July 2018

Arterial hypertension (AH) is one of the most important risk factors for development of ischemic heart disease; thus, control of AH and effective treatment are of great importance. Since arterial hypertension is commonly referred as asymptomatic condition, the question whether hypertensive condition is associated with the change of well-being and health-related quality of life is still debatable. The aim of the study. To evaluate the impact of duration and treatment of AH on health-related quality of life. Material and methods. The contingent of the study consisted of patients who arrived for a cardiologist’s consultation at the Clinic of Cardiology, Hospital of Kaunas University of Medicine. The patients were randomly selected for the study. The inclusion criteria were as follows: diagnosed arterial hypertension, diabetes mellitus, and the metabolic syndrome. Diagnosed ischemic heart disease (chronic and acute coronary syndromes and their complications) and severe concomitant diseases were exclusion criteria. A total of 101 patients (19 males and 82 females) met the inclusion criteria and consented to participate in the study. Their mean age was 58.03±5.63 years. The patients’ quality of life was evaluated using the Medical Outcomes Study short form 36-item questionnaire (SF-36 questionnaire), which comprises 36 questions grouped into eight domains. The questionnaire was filled in by the subjects independently. Other methods applied to the study were inquiry (for the evaluation of risk factors, lifestyle, and medical history), analysis of medical documents (cholesterol levels and glycemia in blood), and objective examination (height, weight, waist circumference, and arterial blood pressure). Results. The subjects with AH showed lower values compared to normotensive patients in the following domains: physical functioning (P=0.014), role limitations due to physical health (P=0.012), energy/vitality (P=0.016), and general health evaluation (P=0.023). We have not determined the differences in quality of life of the patients whose AH was regulated if compared to those patients without AH. The patients whose treatment of AH was not effective reported lower quality of life in the following SF-36 domains: physical functioning (P=0.003), role limitations due to physical health (P=0.003), general evaluation of health (P=0.017), energy/vitality (P=0.008), and emotional status (P=0.015), if compared to the patients without AH. Conclusions. Patients with AH reported lower quality of life in the following domains: physical functioning, role limitations due to physical health, energy/vitality, and general evaluation of health. Compared to patients without AH, the quality of life of the patients who had the effective treatment did not differ, whereas patients with ineffective treatment had the lower quality of life. Functioning is more statistically significantly limited due to physical health in patients with AH.
Myrianthus arboreus P. Beauv (Cecropiaceae) Extracts Accelerates Sexual Maturation, and Increases Fertility Index and Gestational Rate in Female Wistar Rats
Authors: Awounfack Charline Florence , Mvondo Marie Alfrede , Zingue Stéphane , Ateba Sylvin Benjamin , Djiogue Sefirin , Megnekou Rosette , Tantoh Ndinteh Derek , Njamen Dieudonné
Published: July 2018

Background: Despite the wide use of leaves of Myrianthus arboreus (Cecropiaceae) in several African countries including Cameroon as food and against amenorrhea and female infertility, it has never been tested for this purpose. Methods: Using immature female Wistar rats, the impact of M. arboreus on the sexual maturation parameters (vaginal opening, ovarian relative weight and follicle maturation, gonadotropins and ovarian hormones serum levels) and fertility index has been evaluated through a 30-day oral administration of aqueous and methanol extracts of leaves at the doses of 20, 110 and 200 g/kg/day. Results: Aqueous extract increased the ovarian relative weight (p < 0.001), progesterone (p < 0.001) and gonadotropins (p < 0.001) serum levels, and induced the maturation of ovarian follicles. The methanol extract additionally induced an early vaginal opening (p < 0.001), uterine growth (p < 0.01) and increased estradiol (p < 0.001) serum levels. The fertility index generally increased following treatments, while the gestation rate remained almost unaffected except at the highest tested dose of M. arboreus extracts where lowest values were observed. Conclusion: Globally, M. arboreus induced an early puberty onset and an increased fertility rate validating at least in part its traditional use for female infertility.
MiR-663, a MicroRNA Linked with Inflammation and Cancer That Is under the Influence of Resveratrol
Authors: Michaille Jean-Jacques , Piurowski Victoria , Rigot Brooke , Kelani Hesham , Fortman Emily C. , Tili Esmerina
Published: July 2018

Resveratrol (trans-3,5,4′-trihydroxystilbene, RSV) is a non-flavonoid dietary polyphenol with antioxidant, anti-inflammatory and anti-cancer properties that is primarily found in red berries. While RSV displays many beneficial effects in vitro, its actual effects in vivo or in animal models remain passionately debated. Recent publications suggest that RSV pleiotropic effects could arise from its capability to regulate the expression and activity of microRNAs, short regulators themselves capable of regulating up to several hundreds of target genes. In particular, RSV increases microRNA miR-663 expression in different human cell lines, suggesting that at least some of its multiple beneficial properties are through the modulation of expression of this microRNA. Indeed, the expression of microRNA miR-663 is reduced in certain cancers where miR-663 is considered to act as a tumor suppressor gene, as well as in other pathologies such as cardiovascular disorders. Target of miR-663 include genes involved in tumor initiation and/or progression as well as genes involved in pathologies associated with chronic inflammation. Here, we review the direct and indirect effects of RSV on the expression of miR-663 and its target transcripts, with emphasise on TGFβ1, and their expected health benefits, and argue that elucidating the molecular effects of different classes of natural compounds on the expression of microRNAs should help to identify new therapeutic targets and design new treatments.