bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes and Stability of Anterior Open Bite Treatment with Skeletal Anchorage in Non-Growing Patients and Adults Compared to the Results of Orthognathic Surgery Procedures: A Systematic Review
Authors: Malara Piotr , Bierbaum Susanne , Malara Beata
Published: December 2021

The objective of this review is to evaluate, on the basis of the available literature, if anterior open bite (AOB) can be successfully treated with the intrusion of molar teeth using skeletal anchorage in non-growing patients and adults and if this treatment modality provides comparable results to those obtained by orthognathic surgery procedures. Methods: A systematic review of published data in major databases from 2000 to 2021 was performed. Results: In total, 92 articles were included in title and abstract screening, and only 16 articles (11 concerning AOB correction by molar intrusion with skeletal anchorage, and five considering AOB treatment by orthognathic surgical intervention) qualified for thorough data extraction and analysis. Conclusions: On the basis of this review, it seems to be possible to obtain successful results for AOB treatment in non-growing patients and adults by means of the intrusion of molar teeth with skeletal anchorage. However, due to the different methods of assessing treatment outcomes used by different authors, it is not possible to state conclusively whether the treatment of AOB by means of molar intrusion with skeletal anchorage provides long-term results that are comparable to orthognathic surgery procedures.
Early Postoperative Pneumothorax Might Not Be ‘True’ Recurrence
Authors: Woo Wongi , Kim Chong Hoon , Kim Bong Jun , Song Seung Hwan , Moon Duk Hwan , Kang Du-Young , Lee Sungsoo
Published: December 2021

Objectives: To date, there is no consensual definition of what constitutes a postoperative recurrence of primary spontaneous pneumothorax (PSP), despite there being many studies reporting a high incidence of recurrence. This study aims to describe the long-term recurrence rates of pneumothorax and to suggest a possible way to differentiate recurrence events based on temporal patterns. Methods: This single-center study retrospectively evaluated all postoperative recurrence of PSP from January 2007 to May 2019. Patients’ demographics, history of pneumothorax, radiologic data, surgical technique, and the time between operation and recurrence were analyzed. Univariate and multivariable analyses were conducted to find potential risk factors related to long-term recurrence. Results: Of the 77 postoperative recurrent cases of pneumothorax, 21 (27.2%) occurred within 30 days after surgery and, thus, were classified as early recurrences (ER), while the remaining cases were classified as late recurrences (LR). There was no difference in preoperative variables between the two groups. However, the rate of incidence of second recurrence (SR), which represented a long-term prognosis, was significantly higher in the LR group (28.6% vs. 4.8%, p = 0.030). On univariate and multivariable analyses, late recurrence was the only significant factor predicting later recurrence events. Conclusion: Postoperative recurrence (PoR) within 30 days had a lower SR rate. Therefore, it might not be a ‘true’ postoperative recurrence with a favorable prognosis. Further studies investigating postoperative recurrence based on temporal patterns would be warranted to improve the classification of PoR.
Early Identification of Resuscitated Patients with a Significant Coronary Disease in Out-of-Hospital Cardiac Arrest Survivors without ST-Segment Elevation
Authors: Youn Chun-Song , Yi Hahn , Kim Youn-Jung , Song Hwan , Kim Namkug , Kim Won-Young
Published: December 2021

This study aimed to develop a machine learning (ML)-based model for identifying patients who had a significant coronary artery disease among out-of-hospital cardiac arrest (OHCA) survivors without ST-segment elevation (STE). This multicenter observational study used data from the Korean Hypothermia Network prospective registry (KORHN-PRO) gathered between October 2015 and December 2018. We used information available before targeted temperature management (TTM) as predictor variables, and the primary outcome was a significant coronary artery lesion in coronary angiography (CAG). Among 1373 OHCA patients treated with TTM, 331 patients without STE who underwent CAG were enrolled. Among them, 127 patients (38.4%) had a significant coronary artery lesion. Four ML algorithms, namely regularized logistic regression (RLR), random forest classifier (RF), CatBoost classifier (CBC), and voting classifier (VC), were used with data collected before CAG. The VC model showed the highest accuracy for predicting significant lesions (area under the curve of 0.751). Eight variables (older age, male, initial shockable rhythm, shorter total collapse duration, higher glucose and creatinine, and lower pH and lactate) were significant to ML models. These results showed that ML models may be useful in developing early predictive tools for identifying high-risk patients with a significant stenosis in CAG.
Characterizing the Effect of Water Content on Small-Strain Shear Modulus of Qiantang Silt
Authors: Zhang Lisha , Zhang Shimin , Liu Xin , Sun Yinsuo
Published: December 2021

Due to the impact of natural and artificial influence, such as waves, tides, and artificial dewatering, the small-strain shear modulus of soils may vary with the water content of soil, causing deformation of excavations and other earth structures. The present study used a resonant column device to investigate the effects of water content, void ratio, and confining pressure on the small-strain shear modulus of a silt extracted from an excavation site near Qiantang River in Hangzhou, China. The test results revealed that the effects of the three factors are not coupled and can be characterized by three individual equations. In particular, the small-strain shear modulus decreases with increasing water content under otherwise similar conditions, which can be characterized by a power function. The classical Hardin’s equation is modified to consider the effect of water content by introducing an additional function of water content.
Trichoderma reesei Contains a Biosynthetic Gene Cluster That Encodes the Antifungal Agent Ilicicolin H
Authors: Shenouda Mary L. , Ambilika Maria , Cox Russell J.
Published: December 2021

The trili biosynthetic gene cluster (BGC) from the well-studied organism Trichoderma reesei was studied by heterologous expression in the fungal host Aspergillus oryzae. Coexpression of triliA and triliB produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by triliC then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by triliD affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the T. reesei trili BGC encodes biosynthesis of this potent antifungal agent. Unexpected A. oryzae shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.
Convolutional Neural Networks for Fully Automated Diagnosis of Cardiac Amyloidosis by Cardiac Magnetic Resonance Imaging
Authors: Agibetov Asan , Kammerlander Andreas , Duca Franz , Nitsche Christian , Koschutnik Matthias , Donà Carolina , Dachs Theresa-Marie , Rettl René , Stria Alessa , Schrutka Lore , Binder Christina , Kastner Johannes , Agis Hermine , Kain Renate , Auer-Grumbach Michaela , Samwald Matthias , Hengstenberg Christian , Dorffner Georg , Mascherbauer Julia , Bonderman Diana
Published: December 2021

Aims: We tested the hypothesis that artificial intelligence (AI)-powered algorithms applied to cardiac magnetic resonance (CMR) images could be able to detect the potential patterns of cardiac amyloidosis (CA). Readers in CMR centers with a low volume of referrals for the detection of myocardial storage diseases or a low volume of CMRs, in general, may overlook CA. In light of the growing prevalence of the disease and emerging therapeutic options, there is an urgent need to avoid misdiagnoses. Methods and Results: Using CMR data from 502 patients (CA: n = 82), we trained convolutional neural networks (CNNs) to automatically diagnose patients with CA. We compared the diagnostic accuracy of different state-of-the-art deep learning techniques on common CMR imaging protocols in detecting imaging patterns associated with CA. As a result of a 10-fold cross-validated evaluation, the best-performing fine-tuned CNN achieved an average ROC AUC score of 0.96, resulting in a diagnostic accuracy of 94% sensitivity and 90% specificity. Conclusions: Applying AI to CMR to diagnose CA may set a remarkable milestone in an attempt to establish a fully computational diagnostic path for the diagnosis of CA, in order to support the complex diagnostic work-up requiring a profound knowledge of experts from different disciplines.
RETRACTED: The Effects of Soundscapes in Relieving Stress in an Urban Park
Authors: Cao Xin , Hsu Yen
Published: December 2021

Urban parks are important urban public spaces that guarantee people recreation, create positive emotions and relieve stress. Emerging research has shown that natural soundscapes are associated with restorative landscapes in urban parks. However, there is still a lack of knowledge on the use of physiological indexes to evaluate the effects of natural sounds versus human-based sounds on stress relief. In this study, the three physiological indexes of skin conductance level, heart rate and heart rate variability were collected in Fuzhou West Lake Park with the help of Ergo LAB data platform, and a soundscape perception evaluation questionnaire was used to assess the degree of soundscape perceptions in the sample sites. The differences in the stress relieving effects of different urban park environments were analysed by applying the median test, the Wilcoxon test was applied to analyse the effects of soundscapes and urban park environments on relieving stress, and regression analysis was used to identify the important factors of restorative soundscapes. The results found that urban park environments provide a certain degree of stress relief, but the stress relieving effects of different urban park environments vary and that natural spaces play an important role in relieving stress. Urban park soundscapes are key to restorative environmental design, with natural sounds such as birdsong and stream sound being important factors of restorative soundscapes.
Protection and Revealing of Traditional Settlements and Cultural Assets, as a Tool for Sustainable Development: The Case of Kythera Island in Greece
Authors: Vythoulka Anastasia , Delegou Ekaterini T. , Caradimas Costas , Moropoulou Antonia
Published: December 2021

Preserving and highlighting cultural heritage is directly related to sustainable development. The adaptive reuse of cultural heritage buildings and traditional settlements can be a core issue in the implementation of a circular economy strategy, especially in remote areas. In this framework, the current study focuses on Kythera, an isolated Greek island. For the analysis of the study area, research in local archives and communication with the municipality and local stakeholders was conducted, while questionnaires addressed to Kythera’s residents and visitors were developed and processed. Thus, both the special features of the island and the needs of the local community were identified, leading to the proposal of three adaptive reuse schemes at a different scale and within a different management model. The first scheme is focused on the institution of “Eghorios Periousia” and proposes the adaptive reuse of the island’s English Schools as focal points for the promotion of Kythera’s identity. The second scheme focuses on the smaller declared traditional settlements and proposes the adaptive reuse of their housing stock as an affordable permanent residence solution. The third scheme focuses on the abandoned neighborhood of Mavrogiorgiannika in the traditional settlement of Karavas and proposes its adaptive reuse as agritourism accommodation facilities.
Imaging of Intracellular and Plasma Membrane Pools of PI(4,5)P2 and PI4P in Human Platelets
Authors: Bura Ana , Jurak Begonja Antonija
Published: December 2021

Phosphoinositides (PIs) are phosphorylated membrane lipids that have a plethora of roles in the cell, including vesicle trafficking, signaling, and actin reorganization. The most abundant PIs in the cell are phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol-4-monophosphate (PI4P). The localization and roles of both PI(4,5)P2 and PI4P are well established, is the broadly accepted methodological approach for their immunocytochemical visualization in different cell compartments in several cell lines. However, not much is known about these PIs in platelets (PLTs), the smallest blood cells that detect vessel wall injury, activate, and stop the bleeding. Therefore, we sought to investigate the localization of PI(4,5)P2 and PI4P in resting and activated PLTs by antibody staining. Here, we show that the intracellular pools of PI(4,5)P2 and PI4P can be detected by the established staining protocol, and these pools can be modulated by inhibitors of OCRL phosphatase and PI4KIIIα kinase. However, although resting PLTs readily stain for the plasma membrane (PM) pools of PI(4,5)P2 and PI4P, just a few activated cells were stained with the established protocol. We show that optimized protocol allows for the visualization of PI(4,5)P2 and PI4P at PM in activated PLTs, which could also be modulated by OCRL and PI4KIIIα inhibitors. We conclude that PI(4,5)P2 and PI4P are more sensitive to lipid extraction by permeabilizing agents in activated than in resting human PLTs, which suggests their different roles during PLT activation.
Fabrication of AlGaN High Frequency Bulk Acoustic Resonator by Reactive RF Magnetron Co-sputtering System
Authors: Chang Yu-Chen , Chen Ying-Chung , Cheng Chien-Chuan
Published: December 2021

In this study, aluminum gallium nitride (AlGaN) thin films are used as the piezoelectric layers to fabricate solidly mounted resonators (SMR) for high frequency acoustic wave devices. AlGaN film is deposited on a Bragg reflector, composed of three pairs of Mo and SiO2 films, through a reactive radio frequency (RF) magnetron co-sputtering system at room temperature. The optimized deposition parameters of AlGaN film have a sputtering power of 175 W for Al target, sputtering power of 25 W for GaN target, N2 flow ratio (N2/Ar + N2) of 60%, and sputtering pressure of 10 mTorr. The obtained AlGaN film has a smooth surface, uniform crystal grains, and strong c-axis orientation. The contents of Al and Ga in the AlGaN film, analyzed by energy dispersive X-ray spectroscopy (EDS) are 81% and 19%, respectively. Finally, the frequency response s11 of the obtained SMR device shows that the center frequency is 3.60 GHz, the return loss is about −8.62 dB, the electromechanical coupling coefficient (kt2) is 2.33%, the quality factor (Q) value is 96.93 and the figure of merit (FoM) value is 2.26.
Polyaniline Synthesized by Different Dopants for Fluorene Detection via Photoluminescence Spectroscopy
Authors: Beygisangchin Mahnoush , Abdul Rashid Suraya , Shafie Suhaidi , Sadrolhosseini Amir Reza
Published: December 2021

The effects of different dopants on the synthesis, optical, electrical and thermal features of polyaniline were investigated. Polyaniline (PANI) doped with p-toluene sulfonic acid (PANI-PTSA), camphor sulphonic acid (PANI-CSA), acetic acid (PANI-acetic acid) and hydrochloric acid (PANI-HCl) was synthesized through the oxidative chemical polymerization of aniline under acidic conditions at ambient temperature. Fourier transform infrared light, X-ray diffraction, UV-visible spectroscopy, field emission scanning electron microscopy, photoluminescence spectroscopy and electrical analysis were used to define physical and structural features, bandgap values, electrical conductivity and type and degree of doping, respectively. Tauc calculation reveals the optical band gaps of PANI-PTSA, PANI-CSA, PANI-acetic acid and PANI-HCl at 3.1, 3.5, 3.6 and 3.9 eV, respectively. With the increase in dopant size, crystallinity is reduced, and interchain separations and d-spacing are strengthened. The estimated conductivity values of PANI-PTSA, PANI-CSA, PANI-acetic acid and PANI-HCl are 3.84 × 101, 2.92 × 101, 2.50 × 10−2, and 2.44 × 10−2 S·cm−1, respectively. Particularly, PANI-PTSA shows high PL intensity because of its orderly arranged benzenoid and quinoid units. Owing to its excellent synthesis, low bandgap, high photoluminescence intensity and high electrical features, PANI-PTSA is a suitable candidate to improve PANI properties and electron provider for fluorene-detecting sensors with a linear range of 0.001–10 μM and detection limit of 0.26 nM.
Vacancy Defects in Ga2O3: First-Principles Calculations of Electronic Structure
Authors: Usseinov Abay , Koishybayeva Zhanymgul , Platonenko Alexander , Pankratov Vladimir , Suchikova Yana , Akilbekov Abdirash , Zdorovets Maxim , Purans Juris , Popov Anatoli I.
Published: December 2021

First-principles density functional theory (DFT) is employed to study the electronic structure of oxygen and gallium vacancies in monoclinic bulk β-Ga2O3 crystals. Hybrid exchange–correlation functional B3LYP within the density functional theory and supercell approach were successfully used to simulate isolated point defects in β-Ga2O3. Based on the results of our calculations, we predict that an oxygen vacancy in β-Ga2O3 is a deep donor defect which cannot be an effective source of electrons and, thus, is not responsible for n-type conductivity in β-Ga2O3. On the other hand, all types of charge states of gallium vacancies are sufficiently deep acceptors with transition levels more than 1.5 eV above the valence band of the crystal. Due to high formation energy of above 10 eV, they cannot be considered as a source of p-type conductivity in β-Ga2O3.
Infection Prevention Mask Consisting of Nanofiber Filter and Habutae Silk Fabrics
Authors: Suekawa Masayo , Hashizume Yuya , Tanoue Shuichi , Uematsu Hideyuki , Yamashita Yoshihiro
Published: December 2021

To reduce skin irritation and allergic symptoms caused by long-term mask use, we produced a mask with a filter effect by laminating nanofibers on habutae silk fabric, a specialty of Japan’s Fukui Prefecture, using the electrospinning method. We investigated the filter characteristics of silk fabrics with different weave structures (habutae, flat crepe, and twill). We found that woven fabrics alone could not sufficiently block particles finer than 1 μm, even when the fabric layers were overlapped. Therefore, we had a nanofiber filter layer fabricated on the surface of habutae fabric by the electrospinning method at a weight of 1 g/m2. The nanofibers removed more than 94% of 0.3 μm-particles, which are similar to the size of virus particles. However, the nanofiber layer was so dense that it caused an increase in pressure drop, so we made the nanofiber layer thinner and fabricated the filter on the surface of the habutae fabric at 0.5 g/m2. A three-dimensional mask consisting of two woven fabrics, one with a nanofiber layer on the inside and the other with a normal woven fabric without a nanofiber layer on the outside, was fabricated and tested on 95 subjects. The subjects reported that the nanofiber habutae masks were more comfortable than nonwoven masks. Moreover, the silk woven masks did not cause allergic symptoms such as skin irritation.
Synthesis of Dense MgB2 Superconductor via In Situ and Ex Situ Spark Plasma Sintering Method
Authors: Dadiel Joseph Longji , Naik Sugali Pavan Kumar , Pęczkowski Paweł , Sugiyama Jun , Ogino Hiraku , Sakai Naomichi , Kazuya Yokoyama , Warski Tymon , Wojcik Anna , Oka Tetsuo , Murakami Masato
Published: December 2021

In this study, high-density magnesium diboride (MgB2) bulk superconductors were synthesized by spark plasma sintering (SPS) under pressure to improve the field dependence of the critical current density (Jc-B) in MgB2 bulk superconductors. We investigated the relationship between sintering conditions (temperature and time) and Jc-B using two methods, ex situ (sintering MgB2 synthesized powder) and in situ (reaction sintering of Mg and B powder), respectively. As a result, we found that higher density with suppressed particle growth and suppression of the formation of coarse particles of MgB4 and MgO were found to be effective in improving the Jc-B characteristics. In the ex situ method, the degradation of MgB2 due to pyrolysis was more severe at temperatures higher than 850 °C. The sample that underwent SPS treatment for a short time at 850 °C showed higher density and less impurity phase in the bulk, which improved the Jc-B properties. In addition, the in situ method showed very minimal impurity with a corresponding improvement in density and Jc-B characteristics for the sample optimized at 750 °C. Microstructural characterization and flux pinning (fP) analysis revealed the possibility of refined MgO inclusions and MgB4 phase as new pinning centers, which greatly contributed to the Jc-B properties. The contributions of the sintering conditions on fP for both synthesis methods were analyzed.
Shear Strength Characteristics of Recycled Concrete Aggregate and Recycled Tire Waste Mixtures from Monotonic Triaxial Tests
Authors: Gabryś Katarzyna , Radzevičius Algirdas , Szymański Alojzy , Šadzevičius Raimondas
Published: December 2021

Recycled concrete aggregate (RCA) is a promising substitute for natural aggregates and the reuse of this material can benefit construction projects both economically and environmentally. RCA has received great attention in recent years in the form of aggregate as well as a geotechnical material of sand size. Next to RCA, another recycled material, which reduces the waste volume and is a part of the present challenges in civil engineering, is tire waste. Despite the good engineering properties of recycled tire waste (RTW), its use is still limited, even after almost 30 years since they were first introduced. To broaden the applicability of reused concrete and rubber, a further understanding of their properties and engineering behavior is required. For this reason, the main subject of this paper is composite materials that consist of anthropogenic soil recycled concrete aggregate (RCA) and crushed pieces of recycled tire waste (RTW). In this study, a series of isotropic consolidated drained triaxial tests were undertaken to characterize the shear strength of eight mixtures of variable grain-size distribution, rubber inclusion (RC), and fine fraction (FF) content. The results show that the introduction of rubber waste leads to changes in the strength parameters of the tested mixtures. Improvements in RCA shear strength were observed, the largest for the mixture M7 with 10% of recycled tire waste. Similarly, the effect of fine fraction content on the angle of internal friction and cohesion was found. Dilation characteristics were observed in all analyzed composites. Based on the results of all tests performed, including physical, geometric, chemical, and mechanical properties of the created composites, it can be stated that the samples would meet local road authority requirements for sub-base applications.
The Recent Progress China Has Made in the Backfill Mining Method, Part II: The Composition and Typical Examples of Backfill Systems
Authors: Li Shuai , Zhao Zeming , Yu Haoxuan , Wang Xinmin
Published: December 2021

The backfill mining method is a widely used mining method in the major mines of China, which can maximize the recovery of mineral resources and protect the environment. Currently, major mines in China are starting to build backfill systems to apply the backfill mining method, therefore, Part II reviews the progress China has made on creating backfill systems, of which the main contents include: (1) Composition of the backfill system common in Chinese mines; (2) Typical examples of the backfill system in China. Finally, Part II serves as a guide to begin a conversation, and to encourage experts and scholars to engage in the research of this field.
Bioactive Phytochemicals with Anti-Aging and Lifespan Extending Potentials in Caenorhabditis elegans
Authors: Okoro Nkwachukwu Oziamara , Odiba Arome Solomon , Osadebe Patience Ogoamaka , Omeje Edwin Ogechukwu , Liao Guiyan , Fang Wenxia , Jin Cheng , Wang Bin
Published: December 2021

In the forms of either herbs or functional foods, plants and their products have attracted medicinal, culinary, and nutraceutical applications due to their abundance in bioactive phytochemicals. Human beings and other animals have employed those bioactive phytochemicals to improve health quality based on their broad potentials as antioxidant, anti-microbial, anti-carcinogenic, anti-inflammatory, neuroprotective, and anti-aging effects, amongst others. For the past decade and half, efforts to discover bioactive phytochemicals both in pure and crude forms have been intensified using the Caenorhabditis elegans aging model, in which various metabolic pathways in humans are highly conserved. In this review, we summarized the aging and longevity pathways that are common to C. elegans and humans and collated some of the bioactive phytochemicals with health benefits and lifespan extending effects that have been studied in C. elegans. This simple animal model is not only a perfect system for discovering bioactive compounds but is also a research shortcut for elucidating the amelioration mechanisms of aging risk factors and associated diseases.
Investigating Antiarthritic Potential of Nanostructured Clove Oil (Syzygium aromaticum) in FCA-Induced Arthritic Rats: Pharmaceutical Action and Delivery Strategies
Authors: Shakeel Faiyaz , Alam Prawez , Ali Abuzer , Alqarni Mohammed H. , Alshetaili Abdullah , Ghoneim Mohammed M. , Alshehri Sultan , Ali Amena
Published: December 2021

The combined application of clove oil in a lipid nanocarrier opens a promising avenue for bone and joints therapy. In this study, we successfully developed a tunable controlled-release lipid platform for the efficient delivery of clove oil (CO) for the treatment of rheumatoid arthritis (RA). The ultra-small nanostructured lipid carriers co-loaded with CO (CONCs) were developed through an aqueous titration method followed by microfluidization. The CONCs appeared to be spherical (particle size of 120 nm), stable (zeta potential of −27 mV), and entrapped efficiently (84.5%). In toluene:acetone:glacial acetic acid (90:9:1 percent v/v/v) solvent systems, high-performance thin layer chromatography (HPTLC) analysis revealed the primary components in CO as eugenol (RF = 0.58). The CONCs greatly increased the therapeutic impact of CO in both in vitro and in vivo biological tests, which was further supported by excellent antiarthritic action. The CONC had an antiarthritic activity that was slightly higher than neat CO and slightly lower than standard, according to our data. The improved formulation inhibited serum lysosomal enzymes and proinflammatory cytokines while also improving hind leg function. This study provides a proof of concept to treat RA with a new strategy utilizing essential oils via nanodelivery.
Rationally Designed Bi2M2O9 (M = Mo/W) Photocatalysts with Significantly Enhanced Photocatalytic Activity
Authors: Wang Fangzhi , Zhou Xiaoyan , Li Jing , He Qiuyue , Zheng Ling , Liu Qing , Chen Yan , Zhang Guizhai , Liu Xintong , Li Hongda
Published: December 2021

Novel Bi2W2O9 and Bi2Mo2O9 with irregular polyhedron structure were successfully synthesized by a hydrothermal method. Compared to ordinary Bi2WO6 and Bi2MoO6, the modified structure of Bi2W2O9 and Bi2Mo2O9 were observed, which led to an enhancement of photocatalytic performance. To investigate the possible mechanism of enhancing photocatalytic efficiency, the crystal structure, morphology, elemental composition, and optical properties of Bi2WO6, Bi2MO6, Bi2W2O9, and Bi2Mo2O9 were examined. UV-Vis diffuse reflectance spectroscopy revealed the visible-light absorption ability of Bi2WO6, Bi2MO6, Bi2W2O9, and Bi2Mo2O9. Photoluminescence (PL) and photocurrent indicated that Bi2W2O9 and Bi2Mo2O9 pose an enhanced ability of photogenerated electron–hole pairs separation. Radical trapping experiments revealed that photogenerated holes and superoxide radicals were the main active species. It can be conjectured that the promoted photocatalytic performance related to the modified structure, and a possible mechanism was discussed in detail.
Sorafenib Nanomicelles Effectively Shrink Tumors by Vaginal Administration for Preoperative Chemotherapy of Cervical Cancer
Authors: Wang Jun , Lv Fengmei , Sun Tao , Zhao Shoujin , Chen Haini , Liu Yu , Liu Zhepeng
Published: December 2021

To investigate the potential of sorafenib (SF) in preoperative chemotherapy for cervical cancer to reduce tumor volume, sorafenib micelles (SF micelles) with good stability and high drug loading were designed. SF micelles were prepared by film hydration followed by the ultrasonic method. The results showed that the SF micelles were spherical with an average particle size of 67.18 ± 0.66 nm (PDI 0.17 ± 0.01), a considerable drug loading of 15.9 ± 0.46% (w/w%) and satisfactory stability in buffers containing plasma or not for at least 2 days. In vitro release showed that SF was gradually released from SF micelles and almost completely released on the third day. The results of in vitro cellular intake, cytotoxicity and proliferation of cervical cancer cell TC-1 showed that SF micelles were superior to sorafenib (Free SF). For intravaginal administration, SF micelles were dispersed in HPMC (SF micelles/HPMC), showed good viscosity sustained-release profiles in vitro and exhibited extended residence in intravaginal in vivo. Compared with SF micelles dispersed in N.S. (SF micelles/N.S.), SF micelles/HPMC significantly reduced tumor size with a tumor weight inhibition rate of 73%. The results suggested that SF micelles had good potential for preoperative tumor shrinkage and improving the quality life of patients.
Chemical Characterization and Biological Evaluation of New Cobalt(II) Complexes with Bioactive Ligands, 2-Picolinehydroxamic Acid and Reduced Schiff Base N-(2-Hydroxybenzyl)alanine, in Terms of DNA Binding and Antimicrobial Activity
Authors: Woźniczka Magdalena , Lichawska Marta , Sutradhar Manas , Chmiela Magdalena , Gonciarz Weronika , Pająk Marek
Published: December 2021

Five new heteroligand cobalt(II) complexes with 2-picolinehydroxamic acid and reduced Schiff base, N-(2-hydroxybenzyl)alanine, were formed in an aqueous solution over a wide pH range. The coordination properties of ligands towards the metal ion were determined using a pH-metric method, and then the speciation model was confirmed by UV–Vis studies. A stacking interaction between the Schiff base phenol ring and the 2-picolinehydroxamic acid pyridine ring was found to improve the stability of the heteroligand species, indicating more effective coordination in mixed-ligand complexes than in their respective binary systems. The antimicrobial properties of heteroligand complexes were determined against Gram-negative and Gram-positive bacteria, as well as fungal strains. The formulation demonstrated the highest bacteriostatic and bactericidal activity (3.65 mM) against two strains of Gram-negative Helicobacter pylori bacteria and towards Candida albicans and Candida glabrata; this is important due to the potential co-existence of these microorganisms in the gastric milieu and their role in the development of gastritis. The binary complexes in the cobalt(II)—2-picolinehydroxamic acid system and 2-picolinehydroxamic acid were not cytotoxic against L929 mouse fibroblasts, neither freshly prepared solutions or after two weeks’ storage. By comparison, the heteroligand complexes within the range 0.91–3.65 mM diminished the metabolic activity of L929 cells, which was correlated with increased damage to cell nuclei. The concentration of the heteroligand species increased over time; therefore, the complexes stored for two weeks exhibited stronger anticellular toxicity than the freshly prepared samples. The complexes formed in an aqueous solution under physiological pH effectively bound to calf thymus DNA in an intercalative manner. This DNA-binding ability may underpin the antimicrobial/antifungal activity of the heteroligand complexes and their ability to downregulate the growth of eukaryotic cells.
Correction: Mudie et al. In Vitro-In Silico Tools for Streamlined Development of Acalabrutinib Amorphous Solid Dispersion Tablets. Pharmaceutics 2021, 13, 1257
Authors: Mudie Deanna M. , Stewart Aaron M. , Rosales Jesus A. , Adam Molly S. , Morgen Michael M. , Vodak David T.
Published: December 2021

The authors wish to make the following corrections to this paper [...]
Combining Human Genetics of Multiple Sclerosis with Oxidative Stress Phenotype for Drug Repositioning
Authors: Olla Stefania , Steri Maristella , Formato Alessia , Whalen Michael B. , Corbisiero Silvia , Agresti Cristina
Published: December 2021

In multiple sclerosis (MS), oxidative stress (OS) is implicated in the neurodegenerative processes that occur from the beginning of the disease. Unchecked OS initiates a vicious circle caused by its crosstalk with inflammation, leading to demyelination, axonal damage and neuronal loss. The failure of MS antioxidant therapies relying on the use of endogenous and natural compounds drives the application of novel approaches to assess target relevance to the disease prior to preclinical testing of new drug candidates. To identify drugs that can act as regulators of intracellular oxidative homeostasis, we applied an in silico approach that links genome-wide MS associations and molecular quantitative trait loci (QTLs) to proteins of the OS pathway. We found 10 drugs with both central nervous system and oral bioavailability, targeting five out of the 21 top-scoring hits, including arginine methyltransferase (CARM1), which was first linked to MS. In particular, the direction of brain expression QTLs for CARM1 and protein kinase MAPK1 enabled us to select BIIB021 and PEITC drugs with the required target modulation. Our study highlights OS-related molecules regulated by functional MS variants that could be targeted by existing drugs as a supplement to the approved disease-modifying treatments.
A Sustainable Alternative for Postharvest Disease Management and Phytopathogens Biocontrol in Fruit: Antagonistic Yeasts
Authors: Hernandez-Montiel Luis G. , Droby Samir , Preciado-Rangel Pablo , Rivas-García Tomás , González-Estrada Ramsés R. , Gutiérrez-Martínez Porfirio , Ávila-Quezada Graciela D.
Published: December 2021

Postharvest diseases of fruits caused by phytopathogens cause losses up to 50% of global production. Phytopathogens control is performed with synthetic fungicides, but the application causes environmental contamination problems and human and animal health in addition to generating resistance. Yeasts are antagonist microorganisms that have been used in the last years as biocontrol agents and in sustainable postharvest disease management in fruits. Yeast application for biocontrol of phytopathogens has been an effective action worldwide. This review explores the sustainable use of yeasts in each continent, the main antagonistic mechanisms towards phytopathogens, their relationship with OMIC sciences, and patents at the world level that involve yeast-based-products for their biocontrol.
Vapour Application of Sage Essential Oil Maintain Tomato Fruit Quality in Breaker and Red Ripening Stages
Authors: Chrysargyris Antonios , Rousos Charalampos , Xylia Panayiota , Tzortzakis Nikolaos
Published: December 2021

Consumers seek safe, high-nutritional-value products, and therefore maintaining fresh produce quality is a fundamental goal in the food industry. In an effort to eliminate chemical-based sanitizing agents, there has been a shift in recent decades toward the usage of eco-friendly, natural solutions (e.g., essential oils-EOs). In the present study, tomato fruits (Solanum lycopersicum L. cv. Dafni) at breaker and red ripening stage were exposed to sage essential oils (EO: 50 μL L−1 or 500 μL L−1) for 2, 7 and 14 days, at 11 °C and 90% relative humidity (RH). Quality-related attributes were examined during (sustain effect—SE) and following (vapour-induced memory effect—ME; seven days vapours + seven days storage) vapour treatment. In breaker tomatoes, EO-enrichment (sustained effect) retained fruit firmness, respiration rates, and ethylene emission in low EO levels (50 μL L−1). In contrast, breaker fruit metabolism sped up in high EO levels of 500 μL L−1, with decreased firmness, increased rates of respiration and ethylene, and effects on antioxidant metabolism. The effects were more pronounced during the storage period of 14 days, comparing to the fruit exposed to common storage-transit practice. In red fruits, the EOs impacts were evidenced earlier (at two and seven days of storage) with increased rates of respiration and ethylene, increased β-carotene, and decreased lycopene content. In both breaker and red ripening fruit, EO application decreased weight losses. Considering the fruits pre-exposed to EOs, quality attributes were more affected in green fruits and affected to a lesser level in the red ones. Furthermore, based on appearance, color, and texture evaluations, organoleptic trials demonstrated an overwhelming preference for EO-treated red fruit during choice tests. EOs had lower effects on total phenolics, acidity, total soluble solids, and fruit chroma, with no specific trend for both breaker and red tomatoes. Natural volatiles may aid to retain fruit quality in parallel with their antimicrobial protection offered during storage and transportation of fresh produce. These effects may persist after the EO is removed from the storage conditions.
Plant Response to Mechanically-Induced Stress: A Case Study on Specialized Metabolites of Leafy Vegetables
Authors: Šic Žlabur Jana , Radman Sanja , Fabek Uher Sanja , Opačić Nevena , Benko Božidar , Galić Ante , Samirić Paola , Voća Sandra
Published: December 2021

Plants have evolved various adaptive mechanisms to environmental stresses, such as sensory mechanisms to detect mechanical stimuli. This plant adaptation has been successfully used in the production practice of leafy vegetables, called mechanical conditioning, for many years, but there is still a lack of research on the effects of mechanically-induced stress on the content of specialized metabolites, or phytochemicals with significant antioxidant activity. Therefore, the aim of this study was to determine the content of specialized metabolites and antioxidant capacity of lettuce and green chicory under the influence of mechanical stimulation by brushing. Mechanically-induced stress had a positive effect on the content of major antioxidants in plant cells, specifically vitamin C, total phenols, and flavonoids. In contrast, no effect of mechanical stimulation was found on the content of pigments, total chlorophylls, and carotenoids. Based on the obtained results, it can be concluded that induced mechanical stress is a good practice in the cultivation of leafy vegetables, the application of which provides high quality plant material with high nutritional potential and significantly higher content of antioxidants and phytochemicals important for human health.
Chemical Composition and Biological Activities of Tunisian Ziziphus lotus Extracts: Evaluation of Drying Effect, Solvent Extraction, and Extracted Plant Parts
Authors: Letaief Touka , Garzoli Stefania , Laghezza Masci Valentina , Mejri Jamel , Abderrabba Manef , Tiezzi Antonio , Ovidi Elisa
Published: December 2021

The Tunisian Ziziphus lotus plant was investigated to determine its phytoconstituents and evaluate its biological activities. In particular, the GC/MS technique was used to describe the chemical composition of Z. lotus active extracts and fractions. Among the obtained extracts, the yields of the dried root methanolic extract (29.80%) and the fruit aqueous extract (48.00%) were the highest ones. The dried root methanolic extract exhibited the highest amount in the total phenolics (186.44 ± 0.26 mg GAE/g DW), total flavonoids (102.50 ± 3.53 mg QE/g DW), and tannins (60.714 ± 2.2 mg catechin/g DW). The root aqueous extracts revealed the highest antioxidant activity with an IC50 of 8.96 ± 0.38 mg/L and 16.46 ± 0.60 mg/L for the ABTS•+ and DPPH• assays, respectively. The total antioxidant capacity was accorded to the methanolic extract of the dried roots with a value of 304.07 ± 1.11 µg AAE/mg. The drying process was found to improve the qualitative and quantitative properties of the Z. lotus extracts. The evaluation of the cytotoxic activity against the SH-SY5Y cell line was carried out using MTT assay. The petroleum ether and dichloromethane extracts of the dried roots showed relevant cytotoxic activities. The thin layer chromatography and the GC-MS/GC-FID analysis led to the identification of the 13-epimanool as a potent cytotoxic compound.
Dynamic Prediction of Chilo suppressalis Occurrence in Rice Based on Deep Learning
Authors: Tan Siqiao , Liang Yu , Zheng Ruowen , Yuan Hongjie , Zhang Zhengbing , Long Chenfeng
Published: December 2021

(1) Background: The striped rice stem borer (SRSB), Chilo suppressalis, has severely diminished the yield and quality of rice in China. A timely and accurate prediction of the rice pest population can facilitate the designation of a pest control strategy. (2) Methods: In this study, we applied multiple linear regression (MLR), gradient boosting decision tree (GBDT), and deep auto-regressive (DeepAR) models in the dynamic prediction of the SRSB population occurrence during the crop season from 2000 to 2020 in Hunan province, China, by using weather factors and time series of related pests. (3) Results: This research demonstrated the potential of the deep learning method used in integrated pest management through the qualitative and quantitative evaluation of a reasonable validating dataset (the average coefficient of determination Rmean2 for the DeepAR, GBDT, and MLR models were 0.952, 0.500, and 0.166, respectively). (4) Conclusions: The DeepAR model with integrated ground-based meteorological variables, time series of related pests, and time features achieved the most accurate dynamic forecasting of the population occurrence quantity of SRSB as compared with MLR and GBDT.
Sequencing SARS-CoV-2 in a Malaria Research Laboratory in Mali, West Africa: The Road to Sequencing the First SARS-CoV-2 Genome in Mali
Authors: Dara Antoine , Kouriba Bourema , Daou Amadou , Sangare Abdoul Karim , Kassogue Djibril , Dara Charles , Djimde Abdoulaye A.
Published: December 2021

Next-generation sequencing (NGS) has become a necessary tool for genomic epidemiology. Even though the utility of genomics in human health has been proved, genomic surveillance has never been as important as during the COVID-19 pandemic. This has been demonstrated by the recent use of genomic surveillance to detect new variants of SARS-CoV-2 in the United Kingdom, South Africa, and Brazil. Until recently, Malian scientists did not have access to any local NGS platform, and samples had to be shipped abroad for sequencing. Here, we report on how we adapted a laboratory setup for Plasmodium research to generate the first complete SARS-CoV-2 genome locally. Total RNA underwent a library preparation using an Illumina TruSeq stranded RNA kit. A metagenomics sequencing was performed on an Illumina MiSeq platform, which was followed by bioinformatic analyses on a local server in Mali. We recovered a full genome of SARS-CoV-2 of 29 kb with an average depth coverage of 200×. We have demonstrated our capacity to generate a high-quality genome with limited resources and highlight the need to develop genomics capacity locally to solve health problems. We discuss challenges related to access to reagents during a pandemic period and propose some home-made solutions.
Adaptation to the Risks of Digitalization: New Survival Trends for States in a Multipolar World
Authors: Ragulina Julia V. , Ukolov Vladimir F. , Shabunevich Oleg V.
Published: December 2021

The purpose is to study the new survival trends for states in a multipolar world, determine the successfulness of adaptation to the digitalization of different growth poles, and develop the applied recommendations to improve the practice of adaptation to the risks of digitalization of these growth poles. Design/methodology/approach. The authors use the methods of economic statistics: variation analysis, trend analysis, correlation analysis, and regression analysis. Findings. The commonness of strategies of adaptation to the risks of digitalization for different poles of the world economy is substantiated, and two universal mechanisms—talent management and development of science—are found. The originality of this research is due to the consideration of digitalization from a new view—from the positions of setting states at the brink of survival due to the aggressive digital competition and high complexity of ensuring global competition in a quickly changing digital landscape. The uniqueness of this research is due to taking into account the specific features in a multipolar world. The practical implementation of the offered recommendations opens future perspectives for more successful survival trends in a multipolar world and the improvement of their adaptation to risks digitalization by 69.91% in G7 countries (on average) and by 88.40% in BRICS countries (on average).
Análise das prescrições de ansiolíticos e antidepressivos antes e durante a pandemia da COVID-19/ Analysis of prescriptions for anxiolytics and antidepressants before and during the COVID-19 Pandemic
Authors: Piga Bruna Maria Fava , Shima Vivian Taciany Bonassoli , Romanich Francine Maery Dias Ferreira
Published: December 2021

A ansiedade e a depressão são as doenças mentais que mais afetam a população, podendo atingir de 10-15% da população mundial. O tratamento é realizado por fármacos ansiolíticos e antidepressivos, que exercem sua função no sistema nervoso central (SNC), aumentando a disponibilidade de neurotransmissores, como dopamina, noradrenalina e serotonina. Acredita-se que em tempos de pandemia como a de COVID-19, iniciada no ano de 2020, a incidência dessas doenças aumentou, e consequentemente o consumo desses fármacos. Por isso esse trabalho teve por objetivo avaliar se houve aumento na dispensação de ansiolíticos e antidepressivos antes e durante a pandemia de COVID-19 em uma farmácia pública e privada do município de Rondon – PR, nos anos de 2019 e 2020.O trabalho foi realizado por meio de um estudo descritivo a partir da análise de dados coletados de arquivos de dispensações de medicamentos ansiolíticos e antidepressivos, registrados na base de dados das farmácias analisadas. A classe terapêutica que sofreu o maior aumento foram os antidepressivos, com aumento de 37% na farmácia privada. Em contrapartida na farmácia pública houve uma redução de 30% das prescrições de antidepressivos, em 2020 comparado ao ano anterior. Em relação aos ansiolíticos, observou-se diminuição de 15% nas dispensações no setor público, enquanto na farmácia comercial foi observada uma redução de apenas 3% no total, apesar de alguns fármacos desta classe terem apresentado um aumento. A partir desses resultados foi possível visualizar a repercussão do isolamento social ocasionado pela pandemia do Coronavírus e o impacto causado na saúde mental da população.