bluuebunny/crossref_metadata_2025 huggingface dataset:
Vicinagearth
Authors: Li Xuelong
Published: June 2024

With the development of science and technology, new safety requirements have arisen in low-altitude airspace and underwater space, such as low-altitude three-dimensional transportation, disaster rescue, marine ranching, underwater monitoring. In this paper, we mainly focus on the safety issues in Vicinagearth Space. A technology framework of Vicinagearth Safety (VS) is proposed therein, which covers low-altitude safety, underwater safety and cross-domain safety. The VS is a cutting-edge interdisciplinary and technical field, which is widely oriented to the cross-integration of many disciplines and technical fields, such as aerospace, oceanography, mechatronics, mathematics, physics, acoustics, optics, biology, life science, information science, computer science, control science, cybersecurity, energy, new materials, artificial intelligence, etc. Therefore, the VS framework is of great significance to the scientific innovation, technical improvement, economic development and social stability.
Development and validation of stability indicating HPTLC method for simultaneous estimation of Dapagliflozin and Linagliptin
Authors: Shukla Anchal , Chhalotiya Usmangani , Shah Dimal , Tandel Jinal , Kachhiya Heta , Parmar Mital
Published: June 2024

For the treatment of Type 2 Diabetes Mellitus, Dapagliflozin selectively inhibits the sodium-glucose co-transporter-2, while Linagliptin competitively and reversibly inhibits dipeptidyl peptidase-4 in a fixed dose combination (1:1). Dapagliflozin and Linagliptin estimation in bulk and tablet formulation was accomplished by the development and validation of a precise and accurate HPTLC method. The procedure used Toluene: Chloroform: Methanol: Triethylamine (7:2:1:0.2 v/v/v) as the mobile phase and HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase used. Dapagliflozin and Linagliptin were determined to have Rf values of 0.23 and 0.40 correspondingly. At 224 nm, densitometric analysis was performed in the absorbance mode. For Dapagliflozin and Linagliptin 200–1200 ng/band were the linear ranges in which the procedure produced results. Forced degradation studies were performed on both Dapagliflozin and Linagliptin active pharmaceutical ingredients like acid hydrolysis, base hydrolysis, chemical oxidation, dry heat and photodegradation studies. Dapagliflozin and Linagliptin are susceptible to acid hydrolysis, base hydrolysis, chemical oxidation while both the chemical substances are stable to dry heat and photolytic studies.
On the Budget and Atmospheric Fate of Sulfur Emissions From Large Volcanic Eruptions
Authors: Scaillet Bruno , Oppenheimer Clive
Published: June 2024

Today, volcanic sulfur emissions into the atmosphere are measured spectroscopically from the ground, air and space. For eruptions prior to the satellite era, two main sulfur proxies are used, the rock and ice core records, as illustrated by Peccia et al. (2023, https://doi.org/10.1029/2023gl103334). The first approach is based on calculations of the sulfur content of the magma, while the second uses traces of sulfur deposited in ice. Both approaches have their limitations. For glaciochemistry, the volcano responsible for a sulfur anomaly is often unknown and the atmospheric pathway by which the sulfur reached the ice uncertain. The petrologic method relies, too, on uncertain estimates of eruption size and a number of geochemical assumptions that are hard to verify. A deeper knowledge of processes occurring both within magma bodies prior to eruption, and within volcanic plumes in the atmosphere is needed to further our understanding of the impacts of volcanism on climate.
Evaluating the Use of Seasonal Surface Displacements and Time‐Variable Gravity to Constrain the Interior of Mars
Authors: Wagner N. L. , James P. B. , Ermakov A. I. , Sori M. M.
Published: June 2024

The mass transport of volatiles on Mars represents a seasonally changing load on the surface of the planet. Like on Earth, as mass is redistributed across the planet, the surface responds in a complex manner becoming displaced downwards or upwards. The magnitude and extent of displacement depend on the properties of the load and mechanical properties of the planetary interior. Based on new estimates of the height variation of the seasonal polar caps (SPCs), we predict local surface displacements of up to tens of millimeters with a strong degree 1 signal throughout the Martian year. The long‐wavelength portion of the displacement is potentially observable, with a magnitude of a few millimeters, located away from the SPC where one could realistically measure it with a landed or orbital mission. We also model the direct contribution of this process to observable time variable gravity, where we find the zonal coefficients to be in line with previous measurements, although with a smaller magnitude. Future measurements of this displacement could be used to help elucidate the composition of the interior of Mars using this process as a probe into the Martian interior. Furthermore, more refined measurements of time‐variable gravity would be a powerful tool in constraining the pole‐to‐pole volatile cycle present on Mars.
Coupling Reservoir Operation and Rainfall‐Runoff Processes for Streamflow Simulation in Watersheds
Authors: Vora Anav , Cai Ximing , Chen Yanan , Li Donghui
Published: June 2024

We assess the overall watershed system representation via fully coupling a generic reservoir operation model with a conceptual rainfall‐runoff model. The performance of the coupled model is evaluated comprehensively by examining watershed outflow simulations, model parameter values, and a key internal flux of the watershed model (here reservoir inflow). Five published generic reservoir operation models are coupled with a watershed rainfall‐runoff model, and results are compared across the coupled models and one additional model called ResIgnore that ignores reservoir operation. Traditional loosely coupled watershed hydrologic models (where calibrated inflow is routed through reservoir operation models) are used as baselines to examine the differences in simulation performance and parameterization obtained from the fully coupled models. We find that fully coupling the Generic Data‐Driven Reservoir Operation Model (GDROM) and the Dynamically Zoned Target Release (DZTR) reservoir operation models with the rainfall‐runoff model obtains robust simulations of watershed outflow with realistic parameterization, suggesting that they can be reliably integrated into large‐scale hydrological models for simulating streamflow in heavily dammed watersheds. Our results also show that compared to ResIgnore, the fully coupled watershed models more accurately simulate the entire distribution of watershed outflow, obtain more realistic values of model parameters, and simulate reservoir inflow with higher accuracy. Finally, we note that the prediction intervals of watershed outflow obtained from the GDROM‐ and DZTR‐based fully coupled models consistently envelop observed watershed outflow across the study watersheds, indicating that GDROM and DZTR can be suitable reservoir components of large‐scale hydrology models.
Martian Dust Storm Spatial‐Temporal Analysis of Tentative Landing Areas for China's Tianwen‐3 Mars Mission
Authors: Tian Yuan , Li Bo , Rong Zhaojin , Qu Shaojie , Chen Shengbo
Published: June 2024

China's first Mars sampling return mission (Tianwen‐3) is designed to launch and retrieve samples around 2030. Three tentative landing areas (TLAs) (Amazonis, Chryse and Utopia Planitiae, i.e., TLA‐A, TLA‐C and TLA‐U) are selected based on elevation <−2,000 m and latitude between 17° and 30°N. As a dominant feature of Martian meteorology, dust storms manifest in all seasons and affect the accuracy and safety of Mars exploration missions. Tianwen‐3's landing, sampling and ascent phases are in the dust storm season. Therefore, analyzing dust storm activity around landing areas is significant for the Tianwen‐3 mission. According to Mars Daily Global Maps taken by Mars Orbiter Camera spanning Martian years 24–28, 2,476 dust storm events around the three TLAs were identified in this research. Dust storm temporal probabilities within TLA‐A, TLA‐C and TLA‐U were calculated as 0%–44.69%, 0%–66.29% and 0%–33.64%, separately. Dust storm spatial probabilities around the TLA‐A, TLA‐C and TLA‐U were computed, with ranges of 0%–10.71%, 0%–14.55% and 0%–19.96% during the T1 period (Ls = 161–309°), and 0%–6.75%, 0%–7.65% and 0%–8.26% during the T2 period (Ls = 342‐55°), respectively. Finally, considering the temporal and spatial distribution of dust storms, we recommend the T2 period as the launch scenario. Three safe periods (Ls = 2–18°, 4–12°, and 356–4°) were assigned for the entry‐descent‐landing (EDL) phase, along with one period (Ls = 45–55°) for the take‐off and ascent phase. Five circular landing zones with dust storm spatial probability <3% were selected for the Tianwen‐3 mission.
Volcanic Flank Collapse, Secondary Sediment Failure and Flow‐Transition: Multi‐Stage Landslide Emplacement Offshore Montserrat, Lesser Antilles
Authors: Kühn Michel , Berndt Christian , Watt Sebastian F. L. , Hornbach Matthew J. , Krastel Sebastian , Sass Kristina , Kutterolf Steffen , Freudenthal Tim , Huhn Katrin , Karstens Jens , Schramm Bettina , Elger Judith , Böttner Christoph , Klaeschen Dirk
Published: June 2024

Volcanic flank collapses, especially those in island settings, have generated some of the most voluminous mass transport deposits on Earth and can trigger devastating tsunamis. Reliable tsunami hazard assessments for flank collapse‐driven tsunamis require an understanding of the complex emplacement processes involved. The seafloor sequence southeast of Montserrat (Lesser Antilles) is a key site for the study of volcanic flank collapse emplacement processes that span subaerial to submarine environments. Here, we present new 2D and 3D seismic data as well as MeBo drill core data from one of the most extensive mass transport deposits offshore Montserrat, which exemplifies multi‐phase landslide deposition from volcanic islands. The deposits reveal emplacement in multiple stages including two blocky volcanic debris avalanches, secondary seafloor failure and a late‐stage erosive density current that carved channel‐like incisions into the hummocky surface of the deposit about 15 km from the source region. The highly erosive density current potentially originated from downslope‐acceleration of fine‐grained material that was suspended in the water column earlier during the slide. Late‐stage erosive turbidity currents may be a more common process following volcanic sector collapse than has been previously recognized, exerting a potentially important control on the observed deposit morphology as well as on the runout and the overall shape of the deposit.
Color coded metadevices toward programmed terahertz switching
Authors: He Weibao , Cheng Xiang’ai , Hu Siyang , Ren Ziheng , Yu Zhongyi , Wan Shun , Hu Yuze , Jiang Tian
Published: June 2024

Terahertz modulators play a critical role in high-speed wireless communication, non-destructive imaging, and so on, which have attracted a large amount of research interest. Nevertheless, all-optical terahertz modulation, an ultrafast dynamical control approach, remains to be limited in terms of encoding and multifunction. Here we experimentally demonstrated an optical-programmed terahertz switching realized by combining optical metasurfaces with the terahertz metasurface, resulting in 2-bit dual-channel terahertz encoding. The terahertz metasurface, made up of semiconductor islands and artificial microstructures, enables effective all-optical programming by providing multiple frequency channels with ultrafast modulation at the nanosecond level. Meanwhile, optical metasurfaces covered in terahertz metasurface alter the spatial light field distribution to obtain color code. According to the time-domain coupled mode theory analysis, the energy dissipation modes in terahertz metasurface can be independently controlled by color excitation, which explains the principle of 2-bit encoding well. This work establishes a platform for all-optical programmed terahertz metadevices and may further advance the application of composite metasurface in terahertz manipulation.
MXene-based all-solid flexible electrochromic microsupercapacitor
Authors: Guo Shanlu , Zhu Ruihe , Chen Jingwei , Liu Weilin , Zhang Yuxiang , Li Jianmin , Li Haizeng
Published: June 2024

With the increasing demand for multifunctional optoelectronic devices, flexible electrochromic energy storage devices are being widely recognized as promising platforms for diverse applications. However, simultaneously achieving high capacitance, fast color switching and large optical modulation range is very challenging. In this study, the MXene-based flexible in-plane microsupercapacitor was fabricated via a mask-assisted spray coating approach. By adding electrochromic ethyl viologen dibromide (EVB) into the electrolyte, the device showed a reversible color change during the charge/discharge process. Due to the high electronic conductivity of the MXene flakes and the fast response kinetics of EVB, the device exhibited a fast coloration/bleaching time of 2.6 s/2.5 s, a large optical contrast of 60%, and exceptional coloration efficiency. In addition, EVB acted as a redox additive to reinforce the energy storage performance; as a result, the working voltage window of the Ti3C2-based symmetric aqueous microsupercapacitor was extended to 1 V. Moreover, the device had a high areal capacitance of 12.5 mF cm−2 with superior flexibility and mechanical stability and showed almost 100% capacitance retention after 100 bending cycles. The as-prepared device has significant potential for a wide range of applications in flexible and wearable electronics, particularly in the fields of camouflage, anticounterfeiting, and displays.
Targeting a disintegrin and metalloprotease (ADAM) 17-CD122 axis enhances CD8+ T cell effector differentiation and anti-tumor immunity
Authors: Sun Lina , Jiao Anjun , Liu Haiyan , Ding Renyi , Yuan Ning , Yang Biao , Zhang Cangang , Jia Xiaoxuan , Wang Gang , Su Yanhong , Zhang Dan , Shi Lin , Sun Chenming , Zhang Aijun , Zhang Lianjun , Zhang Baojun
Published: June 2024

CD8+ T cell immune responses are regulated by multi-layer networks, while the post-translational regulation remains largely unknown. Transmembrane ectodomain shedding is an important post-translational process orchestrating receptor expression and signal transduction through proteolytic cleavage of membrane proteins. Here, by targeting the sheddase A Disintegrin and Metalloprotease (ADAM)17, we defined a post-translational regulatory mechanism mediated by the ectodomain shedding in CD8+ T cells. Transcriptomic and proteomic analysis revealed the involvement of post-translational regulation in CD8+ T cells. T cell-specific deletion of ADAM17 led to a dramatic increase in effector CD8+ T cell differentiation and enhanced cytolytic effects to eliminate pathogens and tumors. Mechanistically, ADAM17 regulated CD8+ T cells through cleavage of membrane CD122. ADAM17 inhibition led to elevated CD122 expression and enhanced response to IL-2 and IL-15 stimulation in both mouse and human CD8+ T cells. Intriguingly, inhibition of ADAM17 in CD8+ T cells improved the efficacy of chimeric antigen receptor (CAR) T cells in solid tumors. Our findings reveal a critical post-translational regulation in CD8+ T cells, providing a potential therapeutic strategy of targeting ADAM17 for effective anti-tumor immunity.
Structural and functional insights into the helicase protein E5 of Mpox virus
Authors: Zhang Weizhen , Liu Yusong , Yang Mengquan , Yang Jie , Shao Zhiwei , Gao Yanqing , Jiang Xinran , Cui Ruixue , Zhang Yixi , Zhao Xin , Shao Qiyuan , Cao Chulei , Li Huili , Li Linxi , Liu Hehua , Gao Haishan , Gan Jianhua
Published: June 2024

Mpox virus (MPXV) can cause mpox in humans. Due to its quick and wide spread in the past two years, mpox has turned into a significant public health concern. Helicase E5 is a multi-domain protein; its primer synthesis and DNA unwinding activity are required for genome uncoating and DNA replication of MPXV. However, the in vitro DNA unwinding activity has never been demonstrated. Here, we report the structural and biochemical studies of MPXV E5, showing that the full-length protein adopts an auto-inhibited conformation. Truncation of the N-terminus can recover the in vitro unwinding activity of E5 towards the forked DNA. Further structural analysis reveals that MPXV E5 shares a conserved mechanism in DNA unwinding and primer synthesis with the homologous proteins. These findings not only advance our understanding on the function of MPXV E5, but also provide a solid basis for the development of anti-poxvirus drugs.
Synthesis and biochemical characterization of naphthoquinone derivatives targeting bacterial histidine kinases
Authors: Ishikawa Teruhiko , Eguchi Yoko , Igarashi Masayuki , Okajima Toshihide , Mita Kohei , Yamasaki Yuri , Sumikura Kaho , Okumura Taisei , Tabuchi Yuna , Hayashi Chigusa , Pasqua Martina , Coluccia Marco , Prosseda Gianni , Colonna Bianca , Kohayakawa Chie , Tani Akiyoshi , Haruta Jun-ichi , Utsumi Ryutaro
Published: June 2024

Waldiomycin is an inhibitor of histidine kinases (HKs). Although most HK inhibitors target the ATP-binding region, waldiomycin binds to the intracellular dimerization domain (DHp domain) with its naphthoquinone moiety presumed to interact with the conserved H-box region. To further develop inhibitors targeting the H-box, various 2-aminonaphthoquinones with cyclic, aliphatic, or aromatic amino groups and naphtho [2,3-d] isoxazole-4,9-diones were synthesized. These compounds were tested for their inhibitory activity (IC50) against WalK, an essential HK for Bacillus subtilis growth, and their minimum inhibitory concentrations (MIC) against B. subtilis. As a result, 11 novel HK inhibitors were obtained as naphthoquinone derivatives (IC50: 12.6–305 µM, MIC: 0.5–128 µg ml−1). The effect of representative compounds on the expression of WalK/WalR regulated genes in B. subtilis was investigated. Four naphthoquinone derivatives induced the expression of iseA (formerly yoeB), whose expression is negatively regulated by the WalK/WalR system. This suggests that these compounds inhibit WalK in B. subtilis cells, resulting in antibacterial activity. Affinity selection/mass spectrometry analysis was performed to identify whether these naphthoquinone derivatives interact with WalK in a manner similar to waldiomycin. Three compounds were found to competitively inhibit the binding of waldiomycin to WalK, suggesting that they bind to the H-box region conserved in HKs and inhibit HK activity.
Carbon source competition within the wound microenvironment can significantly influence infection progression
Authors: Maslova Evgenia , EisaianKhongi Lara , Rigole Petra , Coenye Tom , McCarthy Ronan R.
Published: June 2024

It is becoming increasingly apparent that commensal skin bacteria have an important role in wound healing and infection progression. However, the precise mechanisms underpinning many of these probiotic interactions remain to be fully uncovered. In this work, we demonstrate that the common skin commensal Cutibacterium acnes can limit the pathogenicity of the prevalent wound pathogen Pseudomonas aeruginosa in vivo. We show that this impact on pathogenicity is independent of any effect on growth, but occurs through a significant downregulation of the Type Three Secretion System (T3SS), the primary toxin secretion system utilised by P. aeruginosa in eukaryotic infection. We also show a downregulation in glucose acquisition systems, a known regulator of the T3SS, suggesting that glucose availability in a wound can influence infection progression. C. acnes is well known as a glucose fermenting organism, and we demonstrate that topically supplementing a wound with glucose reverses the probiotic effects of C. acnes. This suggests that introducing carbon source competition within the wound microenvironment may be an effective way to prevent or limit wound infection.
Microbial strategies driving low concentration substrate degradation for sustainable remediation solutions
Authors: Yin Qidong , He Kai , Collins Gavin , De Vrieze Jo , Wu Guangxue
Published: June 2024

Microbial metabolism upholds a fundamental role in the sustainability of water ecosystems. However, how microorganisms surviving in low-concentration substrate water environments, including the existence of emerging compounds of interest, remains unclear. In this review, microbial strategies for concentrating, utilizing, and metabolizing of low concentration substrates were summarized. Microorganisms develop substrate-concentrating strategies at both the cell and aggregate levels in substrate-limited settings. Following, microbial uptake and transport of low-concentration substrates are facilitated by adjusting physiological characteristics and shifting substrate affinities. Finally, metabolic pathways, such as mixed-substrate utilization, syntrophic metabolism, dynamic response to nutrient variation, and population density-based mechanisms allow microorganisms to efficiently utilize low-concentration substrates and to adapt to challenging oligotrophic environments. All these microbial strategies will underpin devising new approaches to tackle environmental challenges and drive the sustainability of water ecosystems, particularly in managing low-concentration contaminants (i.e., micropollutants).
Spatial control of perilacunar canalicular remodeling during lactation
Authors: Sieverts Michael , Yee Cristal , Nemani Minali , Parkinson Dilworth Y. , Alliston Tamara , Acevedo Claire
Published: June 2024

Osteocytes locally remodel their surrounding tissue through perilacunar canalicular remodeling (PLR). During lactation, osteocytes remove minerals to satisfy the metabolic demand, resulting in increased lacunar volume, quantifiable with synchrotron X-ray radiation micro-tomography (SRµCT). Although the effects of lactation on PLR are well-studied, it remains unclear whether PLR occurs uniformly throughout the bone and what mechanisms prevent PLR from undermining bone quality. We used SRµCT imaging to conduct an in-depth spatial analysis of the impact of lactation and osteocyte-intrinsic MMP13 deletion on PLR in murine bone. We found larger lacunae undergoing PLR are located near canals in the mid-cortex or endosteum. We show lactation-induced hypomineralization occurs 14 µm away from lacunar edges, past a hypermineralized barrier. Our findings reveal that osteocyte-intrinsic MMP13 is crucial for lactation-induced PLR near lacunae in the mid-cortex but not for whole-bone resorption. This research highlights the spatial control of PLR on mineral distribution during lactation.
The effective treatment of dye-containing simulated wastewater by using the cement kiln dust as an industrial waste adsorbent
Authors: Syala Eslam , Sadik Wagih A. , El-Demerdash Abdel-Ghaffar M. , Mekhamer Waffa , El-Rafey M. Essam
Published: June 2024

This study compares the adsorption behavior of both Methylene Blue (MB) and Congo Red (CR) dyes on the surfaces of cement kiln dust (CKD) powder from the experimentally simulated wastewater solution. The cement kiln dust powder was characterized using X-ray Fluorescence (XRF), X-ray diffraction (XRD), N2 adsorption–desorption Brunauer–Emmett–Teller (BET), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) tests. The adsorption for such dyes was studied under varying mixing contact times, temperatures, and pH as well as various initial concentrations of both dyes and adsorbent using the batch mode experiments. Pseudo-first-order, pseudo-second-order, and intraparticle diffusion models were applied, and the results revealed that the pseudo-second-order fitted well to the kinetic data. Thermodynamic parameters stated that the adsorption process was endothermic. Studying Linear and nonlinear forms of Langmuir and Freundlich's adsorption isotherms revealed that the adsorption process was followed by both homogeneous mono-layer and heterogeneous multilayer coverage on the active sites of cement kiln dust particles. The data showed that the adsorption capacities of the methylene blue and Congo red dyes were 58.43 and 123.42 mg/g, respectively and cement kiln dust is an adsorbent with little cost for the treatment of wastewater.
Prediction and reliability analysis of shear strength of RC deep beams
Authors: Megahed Khaled
Published: June 2024

This study explores machine learning (ML) capabilities for predicting the shear strength of reinforced concrete deep beams (RCDBs). For this purpose, eight typical machine-learning models, i.e., symbolic regression (SR), XGBoost (XGB), CatBoost (CATB), random forest (RF), LightGBM, support vector regression (SVR), artificial neural networks (ANN), and Gaussian process regression (GPR) models, are selected and compared based on a database of 840 samples with 14 input features. The hyperparameter tuning of the introduced ML models is performed using the Bayesian optimization (BO) technique. The comparison results show that the CatBoost model is the most reliable and accurate ML model (R2 = 0.997 and 0.947 in the training and testing sets, respectively). In addition, simple and practical design expressions for RCDBs have been proposed based on the SR model with a physical meaning and acceptable accuracy (an average prediction-to-test ratio of 0.935 and a standard deviation of 0.198). Meanwhile, the shear strength predicted by ML models was then compared with classical mechanics-driven shear models, including two prominent practice codes (i.e., ACI318, EC2) and two previous mechanical models, which indicated that the ML approach is highly reliable and accurate over conventional methods. In addition, a reliability-based design was conducted on two ML models, and their reliability results were compared with those of two code standards. The findings revealed that the ML models demonstrate higher reliability compared to code standards.
Multi-scale entropy assessment of magnetoencephalography signals in schizophrenia
Authors: Wolfson E. J. , Fekete T. , Loewenstein Y. , Shriki O.
Published: June 2024

Schizophrenia is a severe disruption in cognition and emotion, affecting fundamental human functions. In this study, we applied Multi-Scale Entropy analysis to resting-state Magnetoencephalography data from 54 schizophrenia patients and 98 healthy controls. This method quantifies the temporal complexity of the signal across different time scales using the concept of sample entropy. Results show significantly higher sample entropy in schizophrenia patients, primarily in central, parietal, and occipital lobes, peaking at time scales equivalent to frequencies between 15 and 24 Hz. To disentangle the contributions of the amplitude and phase components, we applied the same analysis to a phase-shuffled surrogate signal. The analysis revealed that most differences originate from the amplitude component in the δ, α, and β power bands. While the phase component had a smaller magnitude, closer examination reveals clear spatial patterns and significant differences across specific brain regions. We assessed the potential of multi-scale entropy as a schizophrenia biomarker by comparing its classification performance to conventional spectral analysis and a cognitive task (the n-back paradigm). The discriminative power of multi-scale entropy and spectral features was similar, with a slight advantage for multi-scale entropy features. The results of the n-back test were slightly below those obtained from multi-scale entropy and spectral features.
Refraining from spontaneous face touch is linked to personality traits, reduced memory performance and EEG changes
Authors: Butz Kevin H. G. , Mueller Stephanie M. , Spille Jente L. , Martin Sven , Grunwald Martin
Published: June 2024

Spontaneous touches of one’s face (sFST) were suggested to serve cognitive-emotional regulation processes. During the pandemic, refraining from face-touching was recommended, yet, accompanying effects and the influence of personal attributes remain unclear. Ninety participants (45 female, 45 male) filled out a questionnaire concerning personality, anxiety screening and ADHD screening. Subsequently, they performed a delayed verbal memory recall task four times. After two times, sixty participants were instructed to refrain from face-touching (experimental group). Thirty participants did not receive behavioral instructions (control group). To identify face-touches and conduct further analysis, Video, EMG, and EEG data were recorded. Two samples were formed, depending on the adherence to completely refrain from face-touching (adherent, non-adherent sample) and compared to each other and the control group. EEG analyses uncovered that refraining from face-touching is accompanied by increased beta-power at sensorimotor sites and, exclusively in the non-adherent sample, at frontal sites. Decreased memory performance was found exclusively in subsamples, who non-adherently touched their face while retaining words. In terms of questionnaire results, lower Conscientiousness and higher ADHD screening scores were revealed by the non-adherent compared to the adherent sample. No differences were found among the subsamples. The presented results indicate that refraining from face-touching is related to personal attributes, accompanied by neurophysiological shifts and for a portion of humans by lower memory performance, supporting the notion that sFST serve processes beyond sensorimotor.
Examination of internal metabolome and VOCs profile of brewery yeast and their mutants producing beer with improved aroma
Authors: Jabłoński Sławomir Jan , Mielko-Niziałek Karolina Anna , Leszczyński Przemysław , Gasiński Alan , Kawa-Rygielska Joanna , Młynarz Piotr , Łukaszewicz Marcin
Published: June 2024

Volatile organic compounds (VOCs) are metabolites pivotal in determining the aroma of various products. A well-known VOC producer of industrial importance is Saccharomyces cerevisiae, partially responsible for flavor of beers and wines. We identified VOCs in beers produced by yeast strains characterized by improved aroma obtained in UV-induced mutagenesis. We observed significant increase in concentration of compounds in strains: 1214uv16 (2-phenylethyl acetate, 2- phenylethanol), 1214uv31 (2-ethyl henxan-1-ol), 1214uv33 (ethyl decanoate, caryophyllene). We observed decrease in production of 2-phenyethyl acetate in strain 1214uv33. Analysis of intracellular metabolites based on 1H NMR revealed that intracellular phenylalanine concentration was not changed in strains producing more phenylalanine related VOCs (1214uv16 and 1214uv33), so regulation of this pathway seems to be more sophisticated than is currently assumed. Metabolome analysis surprisingly showed the presence of 3-hydroxyisobutyrate, a product of valine degradation, which is considered to be absent in S. cerevisiae. Our results show that our knowledge of yeast metabolism including VOC production has gaps regarding synthesis pathways for individual metabolites and regulation mechanisms. Detailed analysis of 1214uv16 and 1214uv33 may enhance our knowledge of the regulatory mechanisms of VOC synthesis in yeast, and analysis of strain 1214uv31 may reveal the pathway of 2-ethyl henxan-1-ol biosynthesis.
Designing prepacked aggregate concrete for improved mechanical properties and its field application in constructing steel tube concrete
Authors: Zhou Xiaojun , Bai Shiming , Zhang Yingda , Xie Lin , Zhou Xianliang
Published: June 2024

Prepacked aggregate concrete (PAC) is made by placing coarse aggregates of various sizes into a formwork and then filling the voids between coarse aggregate and grout. The mechanical performance of PAC is dominated by the compactness due to grout filling, but few study considered the pouring methods and grout performance synchronously. The coupled effect of pouring methods and grout performance on the compactness of PAC is investigated in this study. The results show that the gravity pouring method is only suitable for grouts with good flowability. The pump pouring method is more widely used. It can be adapted to grout with poorer fluidity and coarse aggregate with greater apparent density. The ultrasonic pulse velocity test method provides a relatively accurate evaluation of the compactness of PAC. Furthermore, due to the enhanced mechanical properties of PAC, the filed application potential in the preparation of steel tube concrete columns has also been confirmed, where the results exhibited that PAC based steel tube concrete contributed to an enhanced ductility and autogenous shrinkage.
Molecular profiling of NOD mouse islets reveals a novel regulator of insulitis onset
Authors: Mathisen Andreas Frøslev , Vacaru Andrei Mircea , Unger Lucas , Lamba Elena Mirela , Mardare Oana-Ana-Maria , Daian Laura Maria , Ghila Luiza , Vacaru Ana-Maria , Chera Simona
Published: June 2024

Non-obese diabetes (NOD) mice are an established, spontaneous model of type 1 diabetes in which diabetes develops through insulitis. Using next-generation sequencing, coupled with pathway analysis, the molecular fingerprint of early insulitis was mapped in a cohort of mice ranging from 4 to 12 weeks of age. The resulting dynamic timeline revealed an initial decrease in proliferative capacity followed by the emergence of an inflammatory signature between 6 and 8 weeks that increased to a regulatory plateau between 10 and 12 weeks. The inflammatory signature is identified by the activation of central immunogenic factors such as Infg, Il1b, and Tnfa, and activation of canonical inflammatory signaling. Analysis of the regulatory landscape revealed the transcription factor Atf3 as a potential novel modulator of inflammatory signaling in the NOD islets. Furthermore, the Hedgehog signaling pathway correlated with Atf3 regulation, suggesting that the two play a role in regulating islet inflammation; however, further studies are needed to establish the nature of this connection.
ANN-based swarm intelligence for predicting expansive soil swell pressure and compression strength
Authors: Jalal Fazal E. , Iqbal Mudassir , Khan Waseem Akhtar , Jamal Arshad , Onyelowe Kennedy , Lekhraj
Published: June 2024

This research suggests a robust integration of artificial neural networks (ANN) for predicting swell pressure and the unconfined compression strength of expansive soils (PsUCS-ES). Four novel ANN-based models, namely ANN-PSO (i.e., particle swarm optimization), ANN-GWO (i.e., grey wolf optimization), ANN-SMA (i.e., slime mould algorithm) alongside ANN-MPA (i.e., marine predators’ algorithm) were deployed to assess the PsUCS-ES. The models were trained using the nine most influential parameters affecting PsUCS-ES, collected from a broader range of 145 published papers. The observed results were compared with the predictions made by the ANN-based metaheuristics models. The efficacy of all these formulated models was evaluated by utilizing mean absolute error (MAE), Nash–Sutcliffe (NS) efficiency, performance index ρ, regression coefficient (R2), root mean square error (RMSE), ratio of RMSE to standard deviation of actual observations (RSR), variance account for (VAF), Willmott’s index of agreement (WI), and weighted mean absolute percentage error (WMAPE). All the developed models for Ps-ES had an R significantly > 0.8 for the overall dataset. However, ANN-MPA excelled in yielding high R values for training dataset (TrD), testing dataset (TsD), and validation dataset (VdD). This model also exhibited the lowest MAE of 5.63%, 5.68%, and 5.48% for TrD, TsD, and VdD, respectively. The results of the UCS model’s performance revealed that R exceeded 0.9 in the TrD. However, R decreased for TsD and VdD. Also, the ANN-MPA model yielded higher R values (0.89, 0.93, and 0.94) and comparatively low MAE values (5.11%, 5.67, and 3.61%) in the case of PSO, GWO, and SMA, respectively. The UCS models witnessed an overfitting problem because the aforementioned R values of the metaheuristics were 0.62, 0.56, and 0.58 (TsD), respectively. On the contrary, no significant observation was recorded in the VdD of UCS models. All the ANN-base models were also tested using the a-20 index. For all the formulated models, maximum points were recorded to lie within ± 20% error. The results of sensitivity as well as monotonicity analyses depicted trending results that corroborate the existing literature. Therefore, it can be inferred that the recently built swarm-based ANN models, particularly ANN-MPA, can solve the complexities of tuning the hyperparameters of the ANN-predicted PsUCS-ES that can be replicated in practical scenarios of geoenvironmental engineering.
Performance evaluation of geopolymer mortars containing waste ferrochrome slag and fly ash for sustainable green building
Authors: Koçyiğit Şermin
Published: June 2024

The aim of the present study, an attempt to shed light on the use of industrial-based wastes as alkali-activated binder (AAB) material is mainly. The present novel research work, the characterization of waste ferrochrome slag (FCS) and the performance of alkali-activated mortar consisting of fly ash (FA) were investigated. The characterization of used materials were carried out using advanced microstructural analysis techniques (XRF, XRD and SEM). A total of thirty two mortars are prepared using FCS (90–60%) and FA (10–40%) with 5 M, 10 M sodium hydroxide (NaOH), Na2SiO3/NaOH (SS/SH = 1 and 2) solution. All specimens were cured in an oven at 70 °C and 100 °C for 24 h. After oven curing, the geopolymer mortars were kept in the laboratory for 28 days and thermal and mechanical tests were applied to them. The A5 mixture (SS/SH = 1 with 10%FA, 90%FCS and 5 M NaOH) was found to be optimum in terms of thermal insulation properties, making it suitable for use in sustainable construction in terms of low energy cost through exterior insulation. The C8 mixture (SS/SH = 1 with 40%FA, 60% FCS and 10 M NaOH) was found to be optimum in terms of strength and durability, making it suitable for use in sustainable construction. As a result, in this study, an optimum mixture of waste FCS and FA was obtained and geopolymer building materials that provide thermal insulation and structural performance and are resistant to external influences were produced.
Age-adjusted 5-factor modified frailty index as a valuable tool for patient selection in bilateral simultaneous total knee arthroplasty
Authors: Yamaguchi Yuichi , Matsumura Yosuke , Fujii Masanori , Ide Shuya , Sakai Tatsuya , Nagamine Satomi , Eto Shuichi , Shimazaki Takafumi , Tajima Tomonori , Mawatari Masaaki
Published: June 2024

Although bilateral simultaneous total knee arthroplasty (BSTKA) is an effective treatment for bilateral knee osteoarthritis, safety concerns and lack of precise patient selection criteria persist. The purpose of this retrospective study was to determine the complication rate and the role of frailty in patient selection for BSTKA. We analyzed data from 434 patients who underwent BSTKA between February 2012 and January 2021, examining demographic factors and preoperative blood test results. Complications occurred in 77 patients (18%), with anemia requiring transfusion being the most common (26 patients, 5.9%). In the univariate analysis, age ≥ 75 years, age-adjusted Charlson Comorbidity Index ≥ 5, age-adjusted 5-factor modified Frailty Index (aamFI-5) ≥ 3, hemoglobin ≤ 11.0 g/dL, albumin ≤ 3.5 g/dL, estimated glomerular filtration rate < 45 ml/dl/1.73 m2, and D-dimer ≥ 2.0 μg/mL contributed to postoperative complications (p < 0.05). Multivariate analysis identified aamFI-5 ≥ 3 as an independent risk factor (p = 0.002). Our findings underscore the practical utility of aamFI-5 in predicting complications after BSTKA, providing valuable guidance to surgeons in the selection of BSTKA candidates and ultimately improving clinical outcomes.
Topical application of simvastatin acid sodium salt and atorvastatin calcium salt in vitiligo patients. Results of the randomized, double-blind EVRAAS pilot study
Authors: Niezgoda Anna , Winnicki Andrzej , Krysiński Jerzy , Niezgoda Piotr , Nowowiejska Laura , Czajkowski Rafał
Published: June 2024

Contemporary treatment of vitiligo remains a great challenge to practitioners. The vast majority of currently conducted clinical trials of modern therapeutic methods are focused on systemic medications, while there is only a very limited number of reports on new topical treatment in vitiligo. With their pleiotropic activities statins turned out to be efficient in the treatment of various autoimmune/autoinflammatory disorders. The randomized, double-blind placebo-controlled study of topical administration of the active forms of simvastatin and atorvastatin has been designed to evaluate their efficacy in patients with vitiligo. The study was registered in clinicaltrials.gov (registration number NCT03247400, date of registration: 11th August 2017). A total of 24 patients with the active form of non-segmental vitiligo were enrolled in the study. The change of absolute area of skin lesions, body surface area and vitiligo area scoring index were evaluated throughout the 12 week application of ointments containing simvastatin and atorvastatin. Measurements were performed with planimetry and processed using digital software. Use of active forms of simvastatin and atorvastatin did not result in a significant repigmentation of the skin lesions throughout the study period. Within the limbs treated with topical simvastatin, inhibition of disease progression was significantly more frequent than in the case of placebo (p = 0.004), while the difference was not statistically significant for atorvastatin (p = 0.082). Further studies of topical simvastatin in vitiligo patients should be considered.
The MYB-like protein MylA contributes to conidiogenesis and conidial germination in Aspergillus nidulans
Authors: Son Ye-Eun , Cho He-Jin , Park Hee-Soo
Published: June 2024

Myeloblastosis (MYB)-like proteins are a family of highly conserved transcription factors in animals, plants, and fungi and are involved in the regulation of mRNA expression of genes. In this study, we identified and characterized one MYB-like protein in the model organism Aspergillus nidulans. We screened the mRNA levels of genes encoding MYB-like proteins containing two MYB repeats in conidia and found that the mRNA levels of four genes including flbD, cicD, and two uncharacterized genes, were high in conidia. To investigate the roles of two uncharacterized genes, AN4618 and AN10944, deletion mutants for each gene were generated. Our results revealed that AN4618 was required for fungal development. Therefore, we further investigated the role of AN4618, named as mylA, encoding the MYB-like protein containing two MYB repeats. Functional studies revealed that MylA was essential for normal fungal growth and development. Phenotypic and transcriptomic analyses demonstrated that deletion of mylA affected stress tolerance, cell wall integrity, and long-term viability in A. nidulans conidia. In addition, the germination rate of the mylA deletion mutant conidia was decreased compared with that of the wild-type conidia. Overall, this study suggests that MylA is critical for appropriate development, conidial maturation, dormancy, and germination in A. nidulans.
Hub stability in the calcium calmodulin-dependent protein kinase II
Authors: Chien Chih-Ta , Puhl Henry , Vogel Steven S. , Molloy Justin E. , Chiu Wah , Khan Shahid
Published: June 2024

The calcium calmodulin protein kinase II (CaMKII) is a multi-subunit ring assembly with a central hub formed by the association domains. There is evidence for hub polymorphism between and within CaMKII isoforms, but the link between polymorphism and subunit exchange has not been resolved. Here, we present near-atomic resolution cryogenic electron microscopy (cryo-EM) structures revealing that hubs from the α and β isoforms, either standalone or within an β holoenzyme, coexist as 12 and 14 subunit assemblies. Single-molecule fluorescence microscopy of Venus-tagged holoenzymes detects intermediate assemblies and progressive dimer loss due to intrinsic holoenzyme lability, and holoenzyme disassembly into dimers upon mutagenesis of a conserved inter-domain contact. Molecular dynamics (MD) simulations show the flexibility of 4-subunit precursors, extracted in-silico from the β hub polymorphs, encompassing the curvature of both polymorphs. The MD explains how an open hub structure also obtained from the β holoenzyme sample could be created by dimer loss and analysis of its cryo-EM dataset reveals how the gap could open further. An assembly model, considering dimer concentration dependence and strain differences between polymorphs, proposes a mechanism for intrinsic hub lability to fine-tune the stoichiometry of αβ heterooligomers for their dynamic localization within synapses in neurons.
Transcriptomic diversity of innate lymphoid cells in human lymph nodes compared to BM and spleen
Authors: Hashemi Elaheh , McCarthy Colleen , Rao Sridhar , Malarkannan Subramaniam
Published: June 2024

Innate lymphoid cells (ILCs) are largely tissue-resident, mostly described within the mucosal tissues. However, their presence and functions in the human draining lymph nodes (LNs) are unknown. Our study unravels the tissue-specific transcriptional profiles of 47,287 CD127+ ILCs within the human abdominal and thoracic LNs. LNs contain a higher frequency of CD127+ ILCs than in BM or spleen. We define independent stages of ILC development, including EILP and pILC in the BM. These progenitors exist in LNs in addition to naïve ILCs (nILCs) that can differentiate into mature ILCs. We define three ILC1 and four ILC3 sub-clusters in the LNs. ILC1 and ILC3 subsets have clusters with high heat shock protein-encoding genes. We identify previously unrecognized regulons, including the BACH2 family for ILC1 and the ATF family for ILC3. Our study is the comprehensive characterization of ILCs in LNs, providing an in-depth understanding of ILC-mediated immunity in humans.
ATP/azobenzene-guanidinium self-assembly into fluorescent and multi-stimuli-responsive supramolecular aggregates
Authors: Abodja Olivier , Touati Nadia , Morel Mathieu , Rudiuk Sergii , Baigl Damien
Published: June 2024

Building stimuli-responsive supramolecular systems is a way for chemists to achieve spatio-temporal control over complex systems as well as a promising strategy for applications ranging from sensing to drug-delivery. For its large spectrum of biological and biomedical implications, adenosine 5’-triphosphate (ATP) is a particularly interesting target for such a purpose but photoresponsive ATP-based systems have mainly been relying on covalent modification of ATP. Here, we show that simply mixing ATP with AzoDiGua, an azobenzene-guanidium compound with photodependent nucleotide binding affinity, results in the spontaneous self-assembly of the two non-fluorescent compounds into photoreversible, micrometer-sized and fluorescent aggregates. Obtained in water at room temperature and physiological pH, these supramolecular structures are dynamic and respond to several chemical, physical and biological stimuli. The presence of azobenzene allows a fast and photoreversible control of their assembly. ATP chelating properties to metal dications enable ion-triggered disassembly and fluorescence control with valence-selectivity. Finally, the supramolecular aggregates are disassembled by alkaline phosphatase in a few minutes at room temperature, resulting in enzymatic control of fluorescence. These results highlight the interest of using a photoswitchable nucleotide binding partner as a self-assembly brick to build highly responsive supramolecular entities involving biological targets without the need to covalently modify them.