bluuebunny/crossref_metadata_2025 huggingface dataset:
Value-retained vs. impacts avoided: the differentiated contributions of remanufacturing, refurbishment, repair, and reuse within a circular economy
Authors: Russell Jennifer D. , Nasr Nabil Z.
Published: November 2022

Value-retention processes (VRPs), a collective term that includes practices of direct reuse, repair, refurbishment, and remanufacturing, can facilitate the cycling of products and components within a circular economy (CE). VRPs are often presented as alternatives to conventional manufacturing and consumption, and as mechanisms for avoiding negative environmental impacts (e.g., landfill) and mitigating issues of material scarcity. However, these practices are typically lumped together under generic ‘reuse’ strategies within sustainable materials management programs and policies. Further, there is a lack of clarity and data regarding how VRPs differ, and the extent to which they contribute to the avoidance of negative environmental and economic impacts. Using novel integrated product-, process, and economy-level models, we quantify select environmental and economic impact metrics for VRPs and conventional manufacturing across six case study products, in two industrialized economies (USA and China). Using this novel methodology, we demonstrate a basis for clear differentiation of VRPs as distinct strategies within a CE, and show that each VRP offers differing forms of value (e.g. cost reduction, labor opportunity, and material retention) and differing degrees of environmental and economic impacts (e.g., primary material requirement, embodied emissions, process emissions). In all cases, the product- and process-level comparative analyses indicate that VRPs present a clear opportunity for significantly reduced environmental impacts, relative to conventional manufacturing. This novel methodology provides an adaptive, comprehensive model that can support the decision of whether or not to engage in VRPs. By quantifying and evaluating VRPs in terms of their relative environmental and economic performance, the distinct avenues, expectations and outcomes for CE can be better integrated across diverse industry and product portfolios (International Resource Panel [29]).
Non-mosaic X monosomy (77,X) in a female dog with signs of virilization
Authors: Szczerbal Izabela , Malek Emilian , Rigillo Antonella , Lukomska Anna , Kacprzak Kamil , Gasparini Stefania , Nowacka-Woszuk Joanna , Stachowiak Monika , Aksoy Mehmet O. , Switonski Marek
Published: November 2022

A 14-month-old female Miniature Poodle dog with an enlarged clitoris and asymmetry in the placement of the teats was subjected to clinical, histopathological, and genetic studies. Macroscopically, the uterus and fallopian tubes appeared normal, while both ovaries were diffusely altered. At histology, the ovarian parenchyma was almost completely effaced by a diffuse hyperplasia of theca cells with atretic primary follicles. Chromosome analysis showed pure (non-mosaic) X monosomy (77,X). This finding was confirmed by the highly sensitive droplet digital PCR (ddPCR) approach. Despite the observed virilization, molecular analysis did not show the presence of Y-linked genes (SRY,ZFY, andTSPY1) in the blood cells or ovary tissue. To the best of our knowledge, this is the first case of X monosomy in a dog associated with virilization.
Synergy of ball milling, microwave irradiation, and deep eutectic solvents for a rapid and selective delignification: walnut shells as model for lignin-enriched recalcitrant biomass
Authors: Ahorsu Richard , Constanti Magda , de María Pablo Domínguez , Medina Francesc
Published: November 2022

The combination of ball milling (BM), microwave irradiation (MI), and deep eutectic solvents (DES) results synergistic for an efficient, selective, and very rapid (10 min) delignification of materials with high lignin content (ca. 50 wt%) such as walnut shells (WS). Lignin is dissolved in the DES, whereas the polysaccharide fractions remain suspended with limited degradation, due to the rapid pretreatment. After ball milling procedure (3 h), biomass loadings in the range of 100–200 g L−1 are selectively delignified in 10 min at 150 °C by using choline chloride:formic acid DES (1:2 molar ratio), rendering lignin yields of 60–80% (ca. ~ 40–60 g lignin L−1). Ball milling, microwave irradiation, and DES systems are much more efficient than ball milling, conventional heating, and DES system. The obtained lignins exhibited similar Fourier-transform infrared spectroscopy (FTIR) profile to that of milled wood lignin (MWL), indicating minimal functional group changes.

Graphical abstract
Governance on lithium mining shareholdings: expanding Environment, Social and Governance (ESG) indicators to economic regulation and raw material politics
Authors: Soberón Bravo Emilio
Published: November 2022

‘Environment, Social and Governance’ (ESG) considerations have received increasing attention as components that inform responsible investments in mining and global material supply. In ESG, governance variables have been mostly regarded as internal firm structures and broad national institutional strength, neglecting closer observations on economic regulation and national raw material politics. Improved governance data to support ESG evaluations should include historical and current relations between public governance bodies and private-sector firms. This paper examines the role of competition regulation agencies in weighing out and mediating relations between the State and multinational private-sector mining firms to unpack components that affect governance in mining. It does so by commenting on empirical data of the purchase held in 2018 of SQM shares—the world’s second largest lithium raw materials supplier—by Tianqi Lithium—one of the largest lithium materials producers in China. The paper discusses the place of equity markets in politics of nation-making and economic regulation in mining. It argues that beyond internal corporate structures, the governance criteria in the ESG profile of mining companies are largely affected by domestic economic regulation maturity, domestic political geography histories of private equity and imageries of corporate shares and natural resources. Furthermore, this paper argues that the effective governance of lithium supply in Chile is affected by political tensions between economic regulation and contractual regimes in mining.
Natural products in cosmetics
Authors: Liu Ji-Kai
Published: November 2022

The global cosmetics market reached US$500 billion in 2017 and is expected to exceed US$800 billion by 2023, at around a 7% annual growth rate. The cosmetics industry is emerging as one of the fastest-growing industries of the past decade. Data shows that the Chinese cosmetics market was US$60 billion in 2021. It is expected to be the world's number one consumer cosmetics market by 2050, with a size of approximately US$450 billion. The influence of social media and the internet has raised awareness of the risks associated with the usage of many chemicals in cosmetics and the health benefits of natural products derived from plants and other natural resources. As a result, the cosmetic industry is now paying more attention to natural products. The present review focus on the possible applications of natural products from various biological sources in skin care cosmetics, including topical care products, fragrances, moisturizers, UV protective, and anti-wrinkle products. In addition, the mechanisms of targets for evaluation of active ingredients in cosmetics and the possible benefits of these bioactive compounds in rejuvenation and health, and their potential role in cosmetics are also discussed.
Common terms of k-Pell numbers and Padovan or Perrin numbers
Authors: Normenyo Benedict Vasco , Rihane Salah Eddine , Togbé Alain
Published: November 2022

Let $$k\ge 2$$

k
≥
2

. A generalization of the well-known Pell sequence is the k-Pell sequence. For this sequence, the first k terms are $$0,\ldots ,0,1$$

0
,
…
,
0
,
1

and each term afterwards is given by the linear recurrence $$\begin{aligned} P_n^{(k)}=2P_{n-1}^{(k)}+P_{n-2}^{(k)}+\cdots +P_{n-k}^{(k)}. \end{aligned}$$

P
n

(
k
)

=
2

P

n
-
1

(
k
)

+

P

n
-
2

(
k
)

+
⋯
+

P

n
-
k

(
k
)

.

In this paper, we extend the previous work (Rihane and Togbé in Ann Math Inform 54:57–71, 2021) and investigate the Padovan and Perrin numbers in the k-Pell sequence.
Trait-based description of the agronomic and usage potential of a range of plantain varieties from Cameroon
Authors: Dépigny Sylvain , Damour Gaëlle
Published: November 2022

A highly diverse range of plantain varieties are cropped in West and Central Africa, and it is essential to enhance knowledge on this diversity to support farmers in their varietal choices. This study aims at proposing a new way to describe a panel of plantain varieties, based on their agronomic and usage potential. The agronomic trait values of nine plantain varieties, including five traditional varieties (Batard, Big Ebanga, Essong, French Clair and Mbouroukou n°3) representative of the diversity of the plantain group, and four plantain-like hybrids (CRBP39, D248, D535 and FHIA21) were recorded at flowering and harvest. The findings revealed very marked inter-varietal variations, in line with the features of plantain morphotaxonomic subgroups, as well as very high intra-varietal variations, especially for the Essong variety. This extent of intra-varietal variation suggests that traditional plantain multiplication methods could favour the emergence of mutations in plantain varieties and hence of high intra-varietal variability. Finally, the monitored agronomic traits were integrated as agronomic and usage potential indicators per variety and morphotaxonomic subgroup, which could support decision-making on plantain varieties.
Associative factors for tracheostomy in patients presenting with stridor or upper airway obstruction
Authors: Sharma P R , Ahmeidat A , Drever S , Shakeel M
Published: November 2022

Objective

This study aimed to identify associative factors for tracheostomy in patients presenting with airway obstruction.

Methods

Data from a tertiary hospital were reviewed to identify patients who presented with airway obstruction between 2009 and 2020. Patient demographics, causative pathology and treatments were analysed.

Results

The study identified 297 admitted patients. Of these, 66 underwent a tracheostomy and formed the ‘tracheostomy’ group and 231 formed the ‘other intervention’ group. The tracheostomy group had a higher mean age (p = 0.003), and higher percentages of males (p = 0.031) and smokers or ex-smokers (p = 0.020), compared to the other intervention group. The tracheostomy group also had a higher number of patients with a malignancy (p < 0.001) compared to the other intervention group.

Conclusion

Being older, male, a previous or current smoker, or developing airway obstruction due to a malignancy were found to be the main associative factors for requiring a tracheostomy.
Impact of studying practical instrumental music on the psychological well-being of disadvantaged university students
Authors: Devroop Karendra
Published: November 2022

The impact of practical instrumental music instruction on students’ psychological and sociological well-being is well documented in research literature. The extent to which these findings hold true for disadvantaged populations is unknown. Previous studies focused on young students with little to no research on disadvantaged young adults at university level. This study investigated the impact of group practical instrumental music instruction on the psychological well-being of disadvantaged university students. It particularly investigated changes in students’ optimism, self-esteem and happiness after participation in a wind ensemble. The study further looked at possible relationships between optimism, self-esteem, happiness and participation in an instrumental music ensemble. Results revealed increases in participant’s optimism, self-esteem and happiness and moderate to strong positive correlations between variables.
The final hospital need in children discharged from a cardiology acute care unit: a single-centre survey study
Authors: Ware Adam L. , Reiter Lauren , Winder Melissa , Kelly Dallin , Marietta Jennifer , Ohsiek Sonja , Ou Zhining , Presson Angela , Bailly David K.
Published: November 2022

Objective:

Children with heart disease may require inpatient care for many reasons, but ultimately have a final reason for hospitalisation prior to discharge. Factors influencing length of stay in paediatric cardiac acute care units have been described but the last reason for hospitalisation has not been studied. Our aim was to describe Final Hospital Need as a novel measure, determine Final Hospital Need in our patients, and describe factors associated with this Need.

Methods:

Single-centre survey design. Discharging providers selected a Final Hospital Need from the following categories: cardiovascular, respiratory, feeding/fluid, haematology/ID, pain/sedation, systems issues, and other/wound issues. Univariable and multivariable analyses were performed separately for outcomes “cardiovascular” and “feeding/fluid.”

Measurements and Results:

Survey response rate was 99% (624 encounters). The most frequent Final Hospital Needs were cardiovascular (36%), feeding/fluid (24%) and systems issues (13%). Probability of Final Hospital Need “cardiovascular” decreased as length of stay increased. Multivariate analysis showed Final Hospital Need “cardiovascular” was negatively associated with aortic arch repair, Norwood procedure, and Final ICU Need “respiratory” and “other.” Final Hospital Need "feeding/fluid” was negatively associated with left-sided valve procedure, but positively associated with final ICU need “respiratory,” and tube feeding at discharge.

Conclusions:

Final Hospital Need is a novel measure that can be predicted by clinical factors including age, Final ICU Need, and type of surgery. Final Hospital Need may be utilised to track changes in clinical care over time and as a target for improvement work.
The Carolingian Answer to the Iconoclastic War and the Birth of Western Art
Authors: Stella Francesco
Published: November 2022

After a long quarrel scattered with persecutions, uprisings, dismissals and replacements of religious authorities, deaths, military expeditions, confiscations and attempts of assassinations in Greece, Italy and other European areas, the Council of Nicaea, in 787, imposed the victory of the iconodules in the Byzantine Empire. The West, especially the Kingdom of the Franks and the Lombards ruled by Charles, later known as Charlemagne, tried to take an official position in the synod of Frankfurt in 794 and in an odd and complex treatise, comprising four books, entitled Opus Caroli, or Libri Carolini, which were recently attributed by Ann Freeman to Theodulf of Orleans, one of the greatest intellectuals of his time. In this work, which we could call the first western treatise on images, the icon is freed from its ritual and cult value, and returned to its artistic use, thus determining, according to some scholars, the larger freedom of figurative representation that characterizes western religious art as compared with the Orthodox one. This stance is followed by a lively debate, involving many authors, the materials of which have not yet been translated and put into full circulation in historical-artistic research.
Diagnostic overshadowing clouding the efficient recognition of pediatric catatonia: a case series
Authors: Reinfeld Samuel , Gill Poonamdeep
Published: November 2022

Catatonia is a neuropsychiatric condition that causes disruption of movement, emotion, and behaviors. Children and adults with underlying psychiatric conditions are particularly susceptible to developing catatonia, which may result in medical and psychiatric complications. Although catatonia research has been growing at a rapid rate in the last 20 years, it continues to be met with inefficiencies in its diagnosis and incertitude in its treatment. In the pediatric population, catatonia is plagued by diagnostic overshadowing, where the catatonia is erroneously attributed to existing pathologies that lead to a prolonged disease state. This paper describes three pediatric patients with catatonia that fell victim to diagnostic overshadowing. More rigorous training and education are imperative to improve the efficient recognition and treatment of children with catatonia.
Building Copyright Confidence Through Community
Authors: Sewell Claire
Published: November 2022

A working knowledge of copyright is essential for academic library staff at all levels if they are to effectively support their users. However, it is an area that causes anxiety amongst staff who often feel underprepared to deal with the questions they receive. The Covid-19 pandemic and the corresponding online shift has highlighted the need for embedded copyright expertise but how do academic libraries equip their staff with the copyright knowledge they need and (perhaps crucially) give them the confidence to use it? Although formal training is available, copyright is a changeable area requiring ongoing education which is no longer practical in the age of limited training budgets. This case study of the approach at Cambridge University Libraries explores some of the potential reasons for staff apprehension around copyright, the range of copyright knowledge and skills needed in an academic library and how a collaborative approach has helped to increase the copyright confidence of staff across the institution. This article has been written by Claire Sewell, Research Support Librarian at the University of Cambridge.
Global trends and regional differences in the burden of anxiety disorders and major depressive disorder attributed to bullying victimisation in 204 countries and territories, 1999–2019: an analysis of the Global Burden of Disease Study
Authors: Hong C. , Liu Z. , Gao L. , Jin Y. , Shi J. , Liang R. , Maimaitiming M. , Ning X. , Luo Y.
Published: November 2022

Aim

This study aimed to analyse the temporal and spatial trends in the burden of anxiety disorders and major depressive disorder related to bullying victimisation on global, regional and country scales.

Methods

Data were from the 2019 Global Burden of Disease (GBD) Study. We assessed the global disability-adjusted life years (DALYs, per 100 000 population) of anxiety disorders and major depressive disorder attributable to bullying victimisation by age, sex and geographical location. The percentage changes in age-standardised rates of DALYs were used to quantify temporal trends, and the annual rate changes across 204 countries and territories were used to present spatial trends. Furthermore, we examined the relationship between the sociodemographic index (SDI) and the burden of anxiety disorders as well as major depressive disorder attributable to bullying victimisation and its spatial and temporal characteristics globally.

Results

From 1990 to 2019, the global DALY rates of anxiety disorders and major depressive disorder attributable to bullying victimisation increased by 23.31 and 26.60%, respectively, with 27.27 and 29.07% for females and 18.88 and 23.84% for males. Across the 21 GBD regions, the highest age-standardised rates of bullying victimisation-related DALYs for anxiety disorders were in North Africa and the Middle East and for major depressive disorder in High-income North America. From 1990 to 2019, the region with the largest percentage increase in the rates of DALYs was High-income North America (54.66% for anxiety disorders and 105.88% for major depressive disorder), whereas the region with the slowest growth rate or largest percentage decline was East Asia (1.71% for anxiety disorders and −25.37% for major depressive disorder). In terms of SDI, this study found overall upward trends of bullying-related mental disorders in areas regardless of the SDI levels, although there were temporary downward trends in some stages of certain areas.

Conclusions

The number and rates of DALYs of anxiety disorders and major depressive disorder attributable to bullying victimisation increased from 1990 to 2019. Effective strategies to eliminate bullying victimisation in children and adolescents are needed to reduce the burden of anxiety disorders and major depressive disorder. Considering the large variations in the burden by SDI and geographic location, future protective actions should be developed based on the specific cultural contexts, development status and regional characteristics of each country.
Directors’ Report: East London Classics Summer School (August 2022)
Authors: Merali-Smith Sarah , Nongbri Alicia
Published: November 2022

This is a review of the 2nd East London Classics Summer School, run in August 2022. We include details of our daily schedule including language sessions, lectures by guest speakers, a visit to King's College London, and we give thanks to those who are supporting our initiative.
Complex skin modes in non-Hermitian coupled laser arrays
Authors: Liu Yuzhou G. N. , Wei Yunxuan , Hemmatyar Omid , Pyrialakos Georgios G. , Jung Pawel S. , Christodoulides Demetrios N. , Khajavikhan Mercedeh
Published: November 2022

From biological ecosystems to spin glasses, connectivity plays a crucial role in determining the function, dynamics, and resiliency of a network. In the realm of non-Hermitian physics, the possibility of complex and asymmetric exchange interactions ($$\left| {\kappa _{ij}} \right| \ne \left| {\kappa _{ji}} \right|$$

κ

i
j

≠

κ

j
i

) between a network of oscillators has been theoretically shown to lead to novel behaviors like delocalization, skin effect, and bulk-boundary correspondence. An archetypical lattice exhibiting the aforementioned properties is that proposed by Hatano and Nelson in a series of papers in late 1990s. While the ramifications of these theoretical works in optics have been recently pursued in synthetic dimensions, the Hatano–Nelson model has yet to be realized in real space. What makes the implementation of these lattices challenging is the difficulty in establishing the required asymmetric exchange interactions in optical platforms. In this work, by using active optical oscillators featuring non-Hermiticity and nonlinearity, we introduce an anisotropic exchange between the resonant elements in a lattice, an aspect that enables us to observe the non-Hermitian skin effect, phase locking, and near-field beam steering in a Hatano–Nelson laser array. Our work opens up new regimes of phase-locking in lasers while shedding light on the fundamental physics of non-Hermitian systems.
Quantification of cephalocaudal progression of jaundice in preterm infants
Authors: Dam-Vervloet Alida J. , de Boer Foky-Anna , Nijholt Ingrid M. , Poot Lieke , Bosschaart Nienke , van Straaten Henrica L. M.
Published: November 2022

Background

The cephalocaudal progression (CCP) of neonatal jaundice is a well-known phenomenon, but quantitative information on CCP in preterm infants is absent. In this study, CCP was quantified in preterm infants as a function of postnatal age and body location.

Methods

5.693 transcutaneous bilirubin (TcB) measurements were performed in 101 preterm infants from birth until postnatal day seven at five body locations (forehead, sternum, hipbone, tibia, ankle). Multi-level linear regression analysis was performed to evaluate the CCP as a function of body location and postnatal age. TcB measurements at all body locations and postnatal days were compared to total serum bilirubin (TSB) levels (N = 1.113).

Results

The overall average change in ratio of TcB compared to forehead was for sternum +0.04 [95% CI −0.02;0.09]; hipbone +0.05 [0.00;0.01]; tibia −0.33 [−0.38;−0.27] and ankle −0.62 [−0.68;−0.57]. No effect modification of CCP by sex, gestational age, birthweight, phototherapy, and TSB was found. The TcB maximally underestimated the TSB at the ankle −79.5 µmol [−0.1;159.2].

Conclusions

CCP is present in preterm infants and is relatively stable over time. Since TcB measurements on the tibia and ankle underestimate TSB significantly, we advise to use only measurement locations cephalic from the tibia; i.e., hipbone, sternum, and forehead.

Impact

Cephalocaudal progression (CCP) of jaundice in preterm infants, assessed by transcutaneous bilirubin (TcB) measurements, is substantial and rather stable over postnatal day 0 to 7.

To the best of our knowledge, this study is the first to investigate CCP of jaundice in preterm infants as a function of postnatal age in preterm infants.

Our results demonstrate that TcB measurements at the tibia and ankle differ from the TSB beyond the clinically used TcB safety margins. We advise to perform TcB measurements only at locations cephalic from the tibia; i.e., hipbone, forehead, and sternum.
Microglia sequelae: brain signature of innate immunity in schizophrenia
Authors: Rodrigues-Neves A. Catarina , Ambrósio António. F. , Gomes Catarina A.
Published: November 2022

Schizophrenia is a psychiatric disorder with significant impact on individuals and society. The current pharmacologic treatment, which principally alleviates psychosis, is focused on neurotransmitters modulation, relying on drugs with severe side effects and ineffectiveness in a significant percentage of cases. Therefore, and due to difficulties inherent to diagnosis and treatment, it is vital to reassess alternative cellular and molecular drug targets. Distinct risk factors – genetic, developmental, epigenetic, and environmental – have been associated with disease onset and progression, giving rise to the proposal of different pathophysiological mechanisms and putative pharmacological targets. Immunity is involved and, particularly microglia – innate immune cells of the central nervous system, critically involved in brain development – have captured attention as cellular players. Microglia undergo marked morphologic and functional alterations in the human disease, as well as in animal models of schizophrenia, as reported in several original papers. We cluster the main findings of clinical studies by groups of patients: (1) at ultra-high risk of psychosis, (2) with a first episode of psychosis or recent-onset schizophrenia, and (3) with chronic schizophrenia; in translational studies, we highlight the time window of appearance of particular microglia alterations in the most well studied animal model in the field (maternal immune activation). The organization of clinical and translational findings based on schizophrenia-associated microglia changes in different phases of the disease course may help defining a temporal pattern of microglia changes and may drive the design of novel therapeutic strategies.
Unconventional short-range structural fluctuations in cuprate superconductors
Authors: Pelc D. , Spieker R. J. , Anderson Z. W. , Krogstad M. J. , Biniskos N. , Bielinski N. G. , Yu B. , Sasagawa T. , Chauviere L. , Dosanjh P. , Liang R. , Bonn D. A. , Damascelli A. , Chi S. , Liu Y. , Osborn R. , Greven M.
Published: November 2022

The interplay between structural and electronic degrees of freedom in complex materials is the subject of extensive debate in physics and materials science. Particularly interesting questions pertain to the nature and extent of pre-transitional short-range order in diverse systems ranging from shape-memory alloys to unconventional superconductors, and how this microstructure affects macroscopic properties. Here we use neutron and X-ray diffuse scattering to uncover universal structural fluctuations in La2-xSrxCuO4 and Tl2Ba2CuO6+δ, two cuprate superconductors with distinct point disorder effects and with optimal superconducting transition temperatures that differ by more than a factor of two. The fluctuations are present in wide doping and temperature ranges, including compositions that maintain high average structural symmetry, and they exhibit unusual, yet simple scaling behaviour. The scaling regime is robust and universal, similar to the well-known critical fluctuations close to second-order phase transitions, but with a distinctly different physical origin. We relate this behaviour to pre-transitional phenomena in a broad class of systems with structural and magnetic transitions, and propose an explanation based on rare structural fluctuations caused by intrinsic nanoscale inhomogeneity. We also uncover parallels with superconducting fluctuations, which indicates that the underlying inhomogeneity plays an important role in cuprate physics.
Climate change and land-use change impacts on future availability of forage grass species for Ethiopian dairy systems
Authors: Brychkova Galina , Kekae Kelebogile , McKeown Peter C. , Hanson Jean , Jones Chris S. , Thornton Philip , Spillane Charles
Published: November 2022

Forage grasses are central feed resources for livestock globally. In Ethiopian dairy systems, they serve as feed sources during both wet and dry seasons, yet escalating climate change could threaten forage supply. Here, we investigate projected climate change impacts on three forage grasses currently recommended for Ethiopian dairy systems. We determine areas of geographical suitability for each species using three climate projections generated by General Circulation Models (GCMs) and calculate their ability to meet predicted dry matter demand under four scenarios for livestock intensification and land availability. By 2050, Buffel grass (Cenchrus ciliaris) is likely to be negatively affected by climate change in regions such as Tigray, while Rhodes grass (Chloris gayana) and Napier grass (Cenchrus purpureus) may have improved suitability under future climates. Our findings suggest that feed demands could theoretically be met by production of these forage grasses under current and future climates. However, if land availability is reduced and herd composition shifts towards higher-productivity exotic breeds, forage resources will not meet cattle demand even with improved agronomic management.
Examining the impact of zinc on horizontal gene transfer in Enterobacterales
Authors: Ekhlas Daniel , Soro Arturo B. , Leonard Finola C. , Manzanilla Edgar G. , Burgess Catherine M.
Published: November 2022

Antimicrobial resistance is one of the main international health concerns for humans, animals, and the environment, and substantial efforts have focused on reducing its development and spread. While there is evidence for correlations between antimicrobial usage and antimicrobial resistance development, specific information on the effect of heavy metal/antimicrobial usage on bacterial conjugation is more limited. The aim of this study was to investigate the effects of zinc and antimicrobials in different concentrations on horizontal gene transfer of an ampicillin resistance gene, using a multi-drug resistant Escherichia coli donor strain and three different Salmonella enterica serovars as recipient strains. Differences in conjugation frequencies for the different Salmonella recipients were observed, independent of the presence of zinc or the antimicrobials. Selective pressure on the recipient strains, in the form of ampicillin, resulted in a decrease in conjugation frequencies, while, the presence of rifampicin resulted in increases. Zinc exposure affected conjugation frequencies of only one of the three recipient strains, thus the effect of zinc on conjugation frequencies seemed to be concentration and strain dependent. Furthermore, differences in growth rates due to plasmid carriage were observed for one of the Salmonella strains.
Association of right precuneus compression with apathy in idiopathic normal pressure hydrocephalus: a pilot study
Authors: Chadani Yoshihiro , Kashibayashi Tetsuo , Yamamoto Takahiro , Tsuda Atsushi , Fujito Ryoko , Akamatsu Masanori , Kamimura Naoto , Takahashi Ryuichi , Yamagami Takuji , Furuya Hirokazu , Ueba Tetsuya , Saito Motoaki , Inoue Keiji , Kazui Hiroaki
Published: November 2022

Apathy is frequently observed in idiopathic normal pressure hydrocephalus (iNPH) and worsens cognitive impairment and gait disturbance. In this study, we evaluated the regions associated with apathy in iNPH using statistical imaging analysis on the whole brain, both in terms of cerebral blood flow and gray matter volume. Twenty-seven patients with iNPH were assigned to two groups based on their scores on the neuropsychiatric inventory items related to apathy; 18 patients were assigned to the group with apathy (iNPH + APA) and 9 to the group without apathy (iNPH − APA). The magnetic resonance images and cerebral blood flow single-photon emission computed tomography data of the two groups were compared using statistical parametric mapping 12. The regional gray matter volume of the right precuneus was significantly larger in the iNPH + APA group than in the iNPH − APA group, but the regional cerebral blood flow in any region of the brain was not significantly different between the two groups. These results suggested that the larger gray matter volume, which is thought to reflect gray matter compression, in the precuneus might be involved in apathy in iNPH.
New evidence of the emergence of the East Asian monsoon in the early Palaeogene
Authors: Su Li-Fei , Zhang Qian-Qian , Sun Yan-Kun , Zhang Shao-Liang , Smith Thierry , Li Cheng-Sen
Published: November 2022

Previous palaeoenvironmental reconstructions have implied that East Asia was dominated by a zonal climate pattern during the Eocene, with an almost latitudinal arid/semiarid band at ~ 30° N. However, this long-standing model has recently been challenged by growing body of multidisciplinary evidence. Some studies indicated that central China was characterized by climatic fluctuations between humid and drier conditions during the Early Eocene, akin to the present East Asian monsoon (EAM) regime. Using palynological assemblages in the Tantou Basin, central China, we quantitatively reconstructed climate changes from the Late Palaeocene to Early Eocene to better understand climate change in central China. Palynological assemblages revealed that the coniferous and broad-leaved mixed forest in this area received no less than 800 mm of annual precipitation and experienced a climate change from warm and wet to relatively cool and dry. According to palaeoclimate curves, a sudden climate change occurred in the Early Eocene, with the mean annual temperature and precipitation decreasing by 5.1 °C and 214.8 mm, respectively, and the climate became very similar to the present climate, which is controlled by the monsoon. Therefore, this significant climate change during the Early Eocene may signal the emergence of the EAM in East Asia.
Cordyceps cicadae and Cordyceps gunnii have closer species correlation with Cordyceps sinensis: from the perspective of metabonomic and MaxEnt models
Authors: Zhang Min , Sun Xiao , Miao Yujing , Li Minhui , Huang Linfang
Published: November 2022

Cordyceps sinensis is a second-class nationally-protected medicinal fungus and functional food. Cordyceps sinensis resources are endangered, and finding new medicinal materials is a fast and economical way to meet the current demonstrated demand, which can effectively solve the shortage of C. sinensis resources. In this study, the metabolite characteristics of Cordyceps were comprehensively revealed by LC-QTOF-MS technology. The maxent model can be used to predict the habitat suitability distribution of Cordyceps and screen out the main climatic factors affecting its distribution. The correlation model between climate factors and chemical components was established by Pearson correlation analysis. Finally, based on the analysis of climate factors and metabolites, we will analyze the high correlation species with C. sinensis, and develop them as possible alternative species of C. sinensis in the future. The results showed that the suitable area of Cordyceps cicadae demonstrated a downward trend, while that of C. sinensis, Cordyceps militaris and Cordyceps gunnii demonstrated an upwards trend. The suitable areas all shifted to the northwest. The temperature seasonality and max temperature of the warmest month are the maximum climatic factors affecting nucleosides. Compared with C. sinensis, the metabolic spectrum similarities of C. cicadae, C. militaris, and C. gunnii were 94.42%, 80.82%, and 91.00%, respectively. Cordyceps sinensis, C. cicadae, and C. gunnii were correlated well for compounds and climate factors. This study will explore whether C. cicadae, C. militaris and C. gunnii can be used as substitutes for C. sinensis. Our results may provide a reference for resource conservation and sustainable utilization of endangered C. sinensis.
Experiment on monitoring leakage of landfill leachate by parallel potentiometric monitoring method
Authors: Hu Xinmin , Han Yalu , Wang Yong , Zhang Xiaopei , Du Lizhi
Published: November 2022

The accumulation of municipal solid waste (MSW) in landfills often becomes a serious pollution source of geological environment and groundwater. The geological environment is the carrier of the landfill, and also the main pollution object of the landfill. The main pollution modes of the landfill site to the surrounding geological environment are purging, flushing, leachate, etc. If the leachate leakage cannot be found and repaired in time, it will cause serious harm to the geological environment and groundwater. The cost of geological environment and groundwater sampling through borehole surveys is high. Therefore, monitoring the seepage path and migration law of leachate is of great significance for determining the pollution range of the landfill site. In this study, by adjusting the grids of different sizes and changing the flow rate of leachate, the monitoring of fluid migration of different types of leachate was strengthened. The results show that the parallel potential monitoring method can quickly reflect the location and number of leachate points and the migration law of leachate. It provides effective reference data for landfill leachate monitoring.
C1QA, C1QB, and GZMB are novel prognostic biomarkers of skin cutaneous melanoma relating tumor microenvironment
Authors: Liang Zhuoshuai , Pan Lingfeng , Shi Jikang , Zhang Lianbo
Published: November 2022

Skin cutaneous melanoma (SKCM) is the most lethal form of skin cancers owing to high invasiveness and high metastatic potential. Tumor microenvironment (TME) provides powerful evidences for discerning SKCM, raising the prospect to identify biomarkers of SKCM. Based on the transcriptome profiles of patients with SKCM and the corresponding clinical information from The Cancer Genome Atlas (TCGA), we used ESTIMATE algorithm to calculate ImmuneScore and StromalScore and identified the TME-Related differentially expressed genes (DEGs), than the intersected TME-Related DEGs were used for subsequent functional enrichment analysis. Protein–protein interaction (PPI) analysis was used to identify the functionality-related DEGs and univariate Cox regression analysis was used to identify the survival-related DEGs. Furthermore, SKCM-related DEGs were identified based on two Gene Expression Omnibus (GEO) datasets. Finally, we intersected functionality-related DEGs, survival-related DEGs, and SKCM-related DEGs, ascertaining that six DEGs (CCL4, CXCL10, CCL5, GZMB, C1QA, and C1QB) function as core TME-related genes (CTRGs). Significant differences of GZMB, C1QA, and C1QB expressions were found in gender and clinicopathologic staging of SKCM. High levels of GZMB, C1QA, and C1QB expressions were associated with favorable prognosis. Gene set enrichment analysis (GSEA) showed that cell–cell interaction, cell behavior, and intracellular signaling transduction may be mainly involved in both C1QA, C1QB and GZMB expressions and metabolism of phospholipid and amino acid, transcription, and translation may be implicated in low GZMB expressions. C1QA, C1QB, and GZMB are novel SKCM-relating CTRGs, providing promising immune-related prognostic biomarkers for SKCM.
Sensory stimulation for upper limb amputations modulates adaptability of cortical large-scale systems and combination of somatosensory and visual inputs
Authors: Ding Keqin , Chen Yunru , Bose Rohit , Osborn Luke E. , Dragomir Andrei , Thakor Nitish V.
Published: November 2022

Touch-like phantom limb sensations can be elicited through targeted transcutaneous electrical nerve stimulation (tTENS) in individuals with upper limb amputation. The corresponding impact of sensory stimulation on cortical activity remains an open question. Brain network research shows that sensorimotor cortical activity is supported by dynamic changes in functional connections between relevant brain regions. These groups of interconnected regions are functional modules whose architecture enables specialized function and related neural processing supporting individual task needs. Using electroencephalographic (EEG) signals to analyze modular functional connectivity, we investigated changes in the modular architecture of cortical large-scale systems when participants with upper limb amputations performed phantom hand movements before, during, and after they received tTENS. We discovered that tTENS substantially decreased the flexibility of the default mode network (DMN). Furthermore, we found increased interconnectivity (measured by a graph theoretic integration metric) between the DMN, the somatomotor network (SMN) and the visual network (VN) in the individual with extensive tTENS experience. While for individuals with less tTENS experience, we found increased integration between DMN and the attention network. Our results provide insights into how sensory stimulation promotes cortical processing of combined somatosensory and visual inputs and help develop future tools to evaluate sensory combination for individuals with amputations.
Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation
Authors: Meyer Sophie , Lauridsen Henrik , Pedersen Kathrine , Andersson Sofie Amalie , van Ooij Pim , Willems Tineke , Berger Rolf M. F. , Ebels Tjark , Jensen Bjarke
Published: November 2022

Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw 1789) as model system of these diseases. Using micro-echocardiography, we assessed the form and function of the heart of the axolotl, an amphibian, and compared this to human DILV (n = 3). The main finding was that both in the axolotl and DILV, blood flows of disparate oxygen saturation can stay separated in a single ventricle. In the axolotl there is a solitary ventricular inlet and outlet, whereas in DILV there are two separate inlets and outlets. Axolotls had a lower resting heart rate compared to DILV (22 vs. 72 beats per minute), lower ejection fraction (47 vs. 58%), and their oxygen consumption at rest was higher than peak oxygen consumption in DILV (30 vs. 17 ml min−1 kg−1). Concerning the ventricular myocardial organization, histology showed trabeculations in ET (n = 5) are much closer to the normal human setting than to the axolotl setting. We conclude that the axolotl heart resembles some aspects of DILV and ET albeit substantial species differences exist.
Characterisation of urban environment and activity across space and time using street images and deep learning in Accra
Authors: Nathvani Ricky , Clark Sierra N. , Muller Emily , Alli Abosede S. , Bennett James E. , Nimo James , Moses Josephine Bedford , Baah Solomon , Metzler A. Barbara , Brauer Michael , Suel Esra , Hughes Allison F. , Rashid Theo , Gemmell Emily , Moulds Simon , Baumgartner Jill , Toledano Mireille , Agyemang Ernest , Owusu George , Agyei-Mensah Samuel , Arku Raphael E. , Ezzati Majid
Published: November 2022

The urban environment influences human health, safety and wellbeing. Cities in Africa are growing faster than other regions but have limited data to guide urban planning and policies. Our aim was to use smart sensing and analytics to characterise the spatial patterns and temporal dynamics of features of the urban environment relevant for health, liveability, safety and sustainability. We collected a novel dataset of 2.1 million time-lapsed day and night images at 145 representative locations throughout the Metropolis of Accra, Ghana. We manually labelled a subset of 1,250 images for 20 contextually relevant objects and used transfer learning with data augmentation to retrain a convolutional neural network to detect them in the remaining images. We identified 23.5 million instances of these objects including 9.66 million instances of persons (41% of all objects), followed by cars (4.19 million, 18%), umbrellas (3.00 million, 13%), and informally operated minibuses known as tro tros (2.94 million, 13%). People, large vehicles and market-related objects were most common in the commercial core and densely populated informal neighbourhoods, while refuse and animals were most observed in the peripheries. The daily variability of objects was smallest in densely populated settlements and largest in the commercial centre. Our novel data and methodology shows that smart sensing and analytics can inform planning and policy decisions for making cities more liveable, equitable, sustainable and healthy.
Associations between subcutaneous adipocyte hypertrophy and nonalcoholic fatty liver disease
Authors: Holmer Magnus , Hagström Hannes , Chen Ping , Danielsson Olof , Aouadi Myriam , Rydén Mikael , Stål Per
Published: November 2022

Adipocyte hypertrophy and expression of adipokines in subcutaneous adipose tissue (SAT) have been linked to steatosis, nonalcoholic steatohepatitis (NASH) and fibrosis in morbidly obese (BMI ≥ 40 kg/m2) subjects. It is unknown if this is also true for subjects with NAFLD with lesser degrees of obesity (BMI < 35 kg/m2). Thirty-two subjects with biopsy-proven NAFLD and 15 non-diabetic controls matched for BMI underwent fine-needle biopsies of SAT. Adipocyte volume was calculated. RNA-sequencing of SAT was performed in a subset of 20 NAFLD patients. Adipocyte volume and gene expression levels were correlated to the presence of NASH or significant fibrosis. Subjects with NAFLD had larger adipocyte volume compared with controls, (1939 pL, 95% CI 1130–1662 vs. 854 pL, 95% CI 781–926, p < 0.001). There was no association between adipocyte volume and the presence of NASH. Gene expression of adipokines previously described to correlate with NASH in morbid obesity, was not associated with NASH or fibrosis. Our results suggest that persons with NAFLD have larger SAT adipocytes compared with controls and that adipocytes are involved in the pathophysiology of hepatic steatosis in NAFLD. However, adipocyte volume was not associated with NASH or fibrosis in NAFLD subjects with varying degrees of obesity.
Hybrid fractal acoustic metamaterials for low-frequency sound absorber based on cross mixed micro-perforated panel mounted over the fractals structure cavity
Authors: Singh Sanjeet Kumar , Prakash Om , Bhattacharya Shantanu
Published: November 2022

The proposed work enumerates a hybrid thin, deep-subwavelength (2 cm) acoustic metamaterials acting as a completely new type of sound absorber, showing multiple broadband sound absorption effects. Based on the fractal distribution of Helmholtz resonator (HRs) structures, integrated with careful design and construct hybrid cross micro-perforated panel (CMPP) that demonstrate broad banding approximately one-octave low-frequency sound absorption behavior. To determine the sound absorption coefficient of this novel type of metamaterial, the equivalent impedance model for the fractal cavity and the micro-perforated Maa’s model for CMPP are both used. We validate these novel material designs through numerical, theoretical, and experimental data. It is demonstrated that the material design possesses superior sound absorption which is primarily due to the frictional losses of the structure imposed on acoustic wave energy. The peaks of different sound absorption phenomena show tunability by adjusting the geometric parameters of the fractal structures like cavity thickness ‘t’, cross perforation diameter of micro perforated panel, etc. The fractal structures and their perforation panel are optimized dimensionally for maximum broadband sound absorption which is estimated numerically. This new kind of fractals cavity integrated with CMPP acoustic metamaterial has many applications as in multiple functional materials with broad-band absorption behavior etc.