bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Experimental Approach to Understand the Transport of Nanodrugs
Authors: Palchoudhury Soubantika , Das Parnab , Ghasemi Amirehsan , Tareq Syed Mohammed , Sengupta Sohini , Han Jinchen , Maglosky Sarah , Almanea Fajer , Jones Madison , Cox Collin , Rao Venkateswar
Published: August 2023

Nanoparticle-based drugs offer attractive advantages like targeted delivery to the diseased site and size and shape-controlled properties. Therefore, understanding the particulate flow of the nanodrugs is important for effective delivery, accurate prediction of required dosage, and developing efficient drug delivery platforms for nanodrugs. In this study, the transport of nanodrugs including flow velocity and deposition is investigated using three model metal oxide nanodrugs of different sizes including iron oxide, zinc oxide, and combined Cu-Zn-Fe oxide synthesized via a modified polyol approach. The hydrodynamic size, size, morphology, chemical composition, crystal phase, and surface functional groups of the water-soluble nanodrugs were characterized via dynamic light scattering, transmission electron microscopy, scanning electron microscopy-energy dispersive X-ray, X-ray diffraction, and fourier transform infrared spectroscopy, respectively. Two different biomimetic flow channels with customized surfaces are developed via 3D printing to experimentally monitor the velocity and deposition of the different nanodrugs. A diffusion dominated mechanism of flow is seen in size ranges 92 nm to 110 nm of the nanodrugs, from the experimental velocity and mass loss profiles. The flow velocity analysis also shows that the transport of nanodrugs is controlled by sedimentation processes in the larger size ranges of 110–302 nm. However, the combined overview from experimental mass loss and velocity trends indicates presence of both diffusive and sedimentation forces in the 110–302 nm size ranges. It is also discovered that the nanodrugs with higher positive surface charges are transported faster through the two test channels, which also leads to lower deposition of these nanodrugs on the walls of the flow channels. The results from this study will be valuable in realizing reliable and cost-effective in vitro experimental approaches that can support in vivo methods to predict the flow of new nanodrugs.
Green Magnetic Nanoparticles CoFe2O4@Nb5O2 Applied in Paracetamol Removal
Authors: Oliveira Jessica R. P. , Ribas Laura S. , Napoli Jose S. , Abreu Eduardo , Diaz de Tuesta Jose L. , Gomes Helder T. , Tusset Angelo M. , Lenzi Giane G.
Published: August 2023

This study describes the synthesis of an innovative nanomaterial (patent application number BR 1020210000317) composed of cobalt ferrite functionalized in niobium pentoxide CoFe2O4@Nb5O2 (CFNb), synthesized via green synthesis using tangerine peel extract. The material emphasizes the combination of a magnetic material (which allows for easy recovery after application) with niobium pentoxide (a metal which is abundant in Brazil). CFNb was applied as a catalyst for the paracetamol (PCT) degradation by photocatalysis. The new materials were characterized through surface and pore analysis (SBET, SEXT, Smic, Vmic, and VTOTAL), photoacoustic spectroscopy (PAS), zero charge point (pHPZC, scanning electron microscopy (SEM/EDS), and X-ray diffraction (XRD). The reaction parameters studied included pH and catalyst concentration. The results indicated that the CFNb nanocatalysts were efficient in the paracetamol degradation, presenting better results in conditions of low pH (close to 2) and low catalyst concentration under irradiation of the 250 W mercury vapor lamp (greater than 28 mW·cm−2) at 60 min of reaction.
Improving Spiking Neural Network Performance with Auxiliary Learning
Authors: Cachi Paolo G. , Ventura Sebastián , Cios Krzysztof J.
Published: August 2023

The use of back propagation through the time learning rule enabled the supervised training of deep spiking neural networks to process temporal neuromorphic data. However, their performance is still below non-spiking neural networks. Previous work pointed out that one of the main causes is the limited number of neuromorphic data currently available, which are also difficult to generate. With the goal of overcoming this problem, we explore the usage of auxiliary learning as a means of helping spiking neural networks to identify more general features. Tests are performed on neuromorphic DVS-CIFAR10 and DVS128-Gesture datasets. The results indicate that training with auxiliary learning tasks improves their accuracy, albeit slightly. Different scenarios, including manual and automatic combination losses using implicit differentiation, are explored to analyze the usage of auxiliary tasks.
Dynamic Generation Method of Highway ETC Gantry Topology Based on LightGBM
Authors: Zou Fumin , Wang Weihai , Cai Qiqin , Guo Feng , Shi Rouyue
Published: August 2023

In Electronic Toll Collection (ETC) systems, accurate gantry topology data are crucial for fair and efficient toll collection. Currently, inaccuracies in the topology data can cause tolls to be based on the shortest route rather than the actual distance travelled, contradicting the ETC system’s purpose. To address this, we adopt a novel Gradient Boosting Decision Tree (GBDT) algorithm, Light Gradient Boosting Machine (LightGBM), to dynamically update ETC gantry topology data on highways. We use ETC gantry and toll booth transaction data from a province in southeast China, where ETC usage is high at 72.8%. From this data, we generate a candidate topology set and extract five key characteristics. We then use Amap API and QGIS map analysis to annotate the candidate set, and, finally, apply LightGBM to train on these features, generating the dynamic topology. Our comparison of LightGBM with 14 other machine learning algorithms showed that LightGBM outperformed the others, achieving an impressive accuracy of 97.6%. This methodology can help transportation departments maintain accurate and up-to-date toll systems, reducing errors and improving efficiency.
Expansion Theory of Deng’s Metric in [0,1]-Topology
Authors: Meng Bin , Chen Peng , Ba Xiaohui
Published: August 2023

The aim of this paper is to focus on a fuzzy metric called Deng’s metric in [0,1]-topology. Firstly, we will extend the domain of this metric function from M0×M0 to M×M, where M0 and M are defined as the sets of all special fuzzy points and all standard fuzzy points, respectively. Secondly, we will further extend this metric to the completely distributive lattice LX and, based on this extension result, we will compare this metric with the other two fuzzy metrics: Erceg’s metric and Yang-Shi’s metric, and then reveal some of its interesting properties, particularly including its quotient space. Thirdly, we will investigate the relationship between Deng’s metric and Yang-Shi’s metric and prove that a Deng’s metric must be a Yang-Shi’s metric on IX, and consequently an Erceg’s metric. Finally, we will show that a Deng’s metric on IX must be Q−C1, and Deng’s metric topology and its uniform structure are Erceg’s metric topology and Hutton’s uniform structure, respectively.
Understanding In-Line Connections Behavior from Experimental and Numerical Analyses on Rectangular and Circular Hollow Section Elements
Authors: Birdean Calin-Ioan , Both Ioan , Mărginean Ioan , Cernescu Anghel
Published: August 2023

Depending on the connection type, especially semi-rigid connections, the analyses of building structures offer accurate results function of the rigidity and ductility. The present paper analyzes the in-line connection of rectangular and circular hollow sections, categorized as semi-rigid connections, suitable for an architectural design of invisible joints. For such connection the standards do not cover an explicit design method. Experimental bending tests were performed on rectangular and circular hollow sections having the end plate fixed inside the profile and bolted by four and one high-strength bolts, respectively. The joint separation represents a serviceability criterion which was monitored using digital image correlation technique. Based on experimental results, a numerical model was validated using the finite element method. After the validation of the numerical model based on the experimental results, a parametric investigation was conducted to study the influence of the access hole, the preload level, the end plate thickness, and the axial force. The results show the small influence of the bolt preload, but the end plate thickness was of major importance. A reduction of the assembly rigidity was also caused by the manhole. The study shows the feasibility of the connection configuration with the end plate positioned inside the hollow profile.
Cost-Effective Imperfect Production-Inventory System under Variable Production Rate and Remanufacturing
Authors: Ganguly Baishakhi , Dey Bikash Koli , Pareek Sarla , Sarkar Biswajit
Published: August 2023

Several industries are facing many challenges in their production systems due to increasing customer demand. Customer demand is growing for products with innovative features that are flexible, good quality, and appealing. This paper presents a flexible production-inventory system that produces multiple parts of a product. Defective products may be produced during the production process. Those defective products are remanufactured immediately after inspection. Limited budget and space constraints are considered, along with product assembly. Based on different distribution functions, non-linear equations are calculated using the Kuhn–Tucker optimization technique. Numerical examples, a graphical representation, and sensitivity analysis are presented in this paper. The solution procedure evaluates the minimization of the total investment based on the χ2 distribution. This study examines electronic products those are more likely to be defective rather than perfect during production.
Secondary Metabolites, Biological Activities, and Industrial and Biotechnological Importance of Aspergillus sydowii
Authors: Ibrahim Sabrin , Mohamed Shaimaa , Alsaadi Baiaan , Althubyani Maryam , Awari Zainab , Hussein Hazem , Aljohani Abrar , Albasri Jumanah , Faraj Salha , Mohamed Gamal
Published: August 2023

Marine-derived fungi are renowned as a source of astonishingly significant and synthetically appealing metabolites that are proven as new lead chemicals for chemical, pharmaceutical, and agricultural fields. Aspergillus sydowii is a saprotrophic, ubiquitous, and halophilic fungus that is commonly found in different marine ecosystems. This fungus can cause aspergillosis in sea fan corals leading to sea fan mortality with subsequent changes in coral community structure. Interestingly, A. sydowi is a prolific source of distinct and structurally varied metabolites such as alkaloids, xanthones, terpenes, anthraquinones, sterols, diphenyl ethers, pyrones, cyclopentenones, and polyketides with a range of bioactivities. A. sydowii has capacity to produce various enzymes with marked industrial and biotechnological potential, including α-amylases, lipases, xylanases, cellulases, keratinases, and tannases. Also, this fungus has the capacity for bioremediation as well as the biocatalysis of various chemical reactions. The current work aimed at focusing on the bright side of this fungus. In this review, published studies on isolated metabolites from A. sydowii, including their structures, biological functions, and biosynthesis, as well as the biotechnological and industrial significance of this fungus, were highlighted. More than 245 compounds were described in the current review with 134 references published within the period from 1975 to June 2023.
Blood Biomarkers of Neonatal Sepsis with Special Emphasis on the Monocyte Distribution Width Value as an Early Sepsis Index
Authors: Mubaraki Murad A. , Faqihi Ayman , AlQhtani Fatmah , Hafiz Taghreed A. , Alalhareth Ahmed , Thagfan Felwa A. , Elshanat Sherif , Abdel-Gaber Rewaida A. , Dkhil Mohamed A.
Published: August 2023

Background and Objectives: Early detection of neonatal sepsis is critical because it is potentially fatal. Therefore, sepsis biomarkers of sufficient sensitivity and specificity are needed. This study aimed to evaluate the utility of peripheral blood parameters as neonatal sepsis biomarkers and the diagnostic performance of the monocyte distribution width (MDW) in sepsis in a neonatal intensive care unit. Materials and Methods: A cross-sectional study was conducted from September 2019 to August 2020 at the King Saud University Medical City in Riyadh, Saudi Arabia. Samples were collected and organised as follows: 77 study cases were subdivided into two subgroups (other health complication (49) and sepsis (28)), and there were 70 controls. The causative microorganisms of neonatal sepsis were isolated. Peripheral blood samples were collected from each neonate in an ethylenediaminetetraacetic acid tube for a complete blood count and a leukocyte differential count. Moreover, the receiver operating characteristic (ROC) curve analysis was used to measure the diagnostic performance of the MDW. Results: The haematological parameters and neonatal sepsis cases had a considerable correlation. The MDW was the most significant haematological parameter. The ROC analysis of the MDW demonstrated that the area under the curve was 0.89 (95% confidence interval: 0.867 to 0.998) with a sensitivity of 89.3%, a specificity of 88.2%, and a negative predictive value of 97.2% at the cut-off point of 23. Conclusions: The use of haematological parameters is feasible and can be performed rapidly. Neonatal sepsis showed a strong correlation with leukopenia, anaemia, thrombocytopenia, and an elevated MDW value. Moreover, the ROC curve analysis confirmed the high diagnostic ability of the MDW in neonatal sepsis prediction.
Breakdown of Phospholipid Asymmetry Triggers ADAM17-Mediated Rescue Events in Cells Undergoing Apoptosis
Authors: Sperrhacke Maria , Leitzke Sinje , Ahrens Björn , Reiss Karina
Published: August 2023

ADAM17, a prominent member of the “Disintegrin and Metalloproteinase” (ADAM) family, controls vital cellular functions through the cleavage of transmembrane substrates, including epidermal growth factor receptor (EGFR) ligands such as transforming growth factor (TGF)-alpha and Epiregulin (EREG). Several ADAM17 substrates are relevant to oncogenesis and tumor growth. We have presented evidence that surface exposure of phosphatidylserine (PS) is pivotal for ADAM17 to exert sheddase activity. The scramblase Xkr8 is instrumental for calcium-independent exposure of PS in apoptotic cells. Xkr8 can be dually activated by caspase-3 and by kinases. In this investigation, we examined whether Xkr8 would modulate ADAM17 activity under apoptotic and non-apoptotic conditions. Overexpression of Xkr8 in HEK293T cells led to significantly increased caspase-dependent as well as PMA-induced release of EREG and TGF-alpha. Conversely, siRNA-mediated downregulation of Xkr8 in colorectal Caco-2 cancer cells led to decreased PS externalization upon induction of apoptosis, which was accompanied by reduced shedding of endogenously expressed EREG and reduced cell survival. We conclude that Xkr8 shares with conventional scramblases the propensity to upmodulate the ADAM-sheddase function. Liberation of growth factors could serve a rescue function in cells on the pathway to apoptotic death.
Improving the Corrosion Resistance of AZ91 Magnesium Alloy by Surface Coating TiO2 Layers
Authors: Wan Diqing , Tang Hao , Sun Yumeng , Zeng Guilin , Dong Shaoyun , Han Guoliang , Wang Yu , Yang Fan , Wang Yongyong
Published: August 2023

This study adopts the sol-gel method to prepare a TiO2 coating on the surface of the AZ91 magnesium alloy, hydrolyse C16H36O4Ti to generate the TiO2 coating and form a film with excellent corrosion resistance on the surface of an AZ91 magnesium alloy. The composition, surface structure and microstructure of the TiO2 coatings are characterised via X-ray diffraction (XRD) and scanning electron microscopy. The corrosion performance of the surface coatings was investigated through hydrogen evolution experiments and electrochemical tests. The results demonstrate that TiO2 sols prepared from a mixture of hydrochloric acid, deionised water, C16H36O4Ti and anhydrous ethanol can form stable layers on the surface of an AZ91 magnesium alloy after heat treatment. The results of hydrogen evolution experiments and electrochemical tests reveal that the TiO2 coating can effectively improve the corrosion resistance of the AZ91 magnesium alloy.
Pearlite Interlamellar Spacing and Vickers Micro-Hardness in the Necking Region of Cold-Drawn Pearlitic Steel Wires
Authors: Toribio Jesús , Ayaso Francisco-Javier , Rodríguez Rocío
Published: August 2023

The final aim of this paper is to study the microstructural changes in the necking region of progressively cold-drawn pearlitic steel wires by means of a thorough and detailed analysis of pearlite interlamellar spacing and Vickers micro-hardness in this special region. To this end, a set of progressively cold-drawn pearlitic steel wires belonging to a real manufacturing chain were subjected to standard tension tests, in such a manner that the tests were interrupted before the final fracture, i.e., the test development was aborted just at the necking instant. The microstructural changes during necking were evaluated by measuring the pearlite interlamellar spacing in the necking region, as well as the Vickers micro-hardness in the different points of it. The study of the afore-said microstructural changes preceding the final fracture was the final aim of the research, intending to determine the local areas in the necking region of the specimens in which microstructural changes are most evident, thereby affecting the local mechanical response of a specific cold-drawn steel at the moment of instability under load control during the standard tension test.
Numerical Simulation on Crack–Inclusion Interaction for Rib-to-Deck Welded Joints in Orthotropic Steel Deck
Authors: Luo Yuan , Liu Xiaofan , Chen Fanghuai , Zhang Haiping , Xiao Xinhui
Published: August 2023

Weld defects such as porosity, inclusion, burn-through, and lack of penetration are difficult to detect and control effectively in an orthotropic steel deck (OSD), which will be a fatigue crack initiation site and lead to several fatigue cracking. The crack growth behavior in defective welded joints is different from that of defect-free joints. This study investigates crack–inclusion interaction for rib-to-deck welded joints in OSDs based on numerical simulation and linear elastic fracture mechanics (LEFM). A refined finite element model of a half U-rib with cracks and inclusions was established by using the FRANC3D-ABAQUS interactive technology. The full processes of the crack–inclusion interaction from approaching and penetrating were accurately simulated. Critical parameters, including the stress intensity factor (SIF), the shape factor, the growth rate, and the growth direction were analyzed. The stiff and soft inclusions amplify and shield the SIF of cracks when the crack grows to the local area of inclusions. During the entire process of crack growth, the soft and stiff inclusion accelerate and inhibit the crack growth, respectively. The stiff inclusion will lead to asymmetric growth of the crack shape, where the portion of the crack away from the inclusions has a higher growth rate. The soft and stiff inclusions will attract and repel the direction of crack growth at the proximal point, respectively.
Tempering Behavior of a Si-Rich Low-Alloy Medium-Carbon Steel
Authors: Borisov Sergey , Borisova Yuliya , Tkachev Evgeniy , Kniaziuk Tatiana , Kaibyshev Rustam
Published: August 2023

Owing to the addition of Si, 0.33C-1.8Si-1.44Mn-0.58Cr steel exhibits a unique tempering behavior. The tempering takes place in two distinct sequential stages that are significantly different from those in steels containing 0.2–0.5 wt.% of Si. Stage I is associated with the precipitation of transition carbides in a paraequilibrium manner, can take place in temperatures ranging from ~200 to ~474 °C, and concurrently increases strength, ductility, and toughness. Stage II is associated with the decomposition of retained austenite to bainitic ferrite and transition carbides. As a result, no significant effect of overlapping of Stage I with Stage II takes place. Stage III does not occur at temperatures below ~474 °C, since the precipitation of cementite in a orthoequilibrium manner is suppressed by the addition of 1.8 wt.% of Si. It was shown that a major portion of carbon atoms redistributes to Cottrell atmospheres under quenching. During low-temperature tempering at 200–400 °C, the precipitation of transition carbides consumes a large portion of carbon atoms, thereby increasing the number of ductile fractures and improving the impact toughness without strength degradation. The formation of chains of cementite particles on boundaries takes place in Stage IV at a tempering temperature of 500 °C. This process results in the full depletion of excess carbon from a ferritic matrix that provides increased ductility and toughness but decreased strength.
Metformin Can Attenuate Beta-Cell Hypersecretion—Implications for Treatment of Children with Obesity
Authors: Wen Quan , Stenlid Rasmus , Chowdhury Azazul Islam , Ciba Iris , Aydin Banu , Cerenius Sara Y. , Manell Hannes , Forslund Anders , Bergsten Peter
Published: August 2023

In children with obesity, insulin hypersecretion is proposed to precede insulin resistance. We investigated if metformin could be used to attenuate insulin secretion from palmitate-treated isolated islets and its implication for children with obesity. Human islets were exposed to palmitate for 0.5 or 1 day, when metformin was introduced. After culture, glucose-stimulated insulin secretion (GSIS) was measured. Children with obesity, who had received metformin for over six months (n = 21, age 13.9 ± 1.8), were retrospectively evaluated. Children were classified as either “reducing” or “increasing” based on the difference between AUC0–120 of insulin during OGTT before and after metformin treatment. In human islets, GSIS increased after culture in palmitate for up to 1 day but declined with continued palmitate exposure. Whereas adding metformin after 1 day of palmitate exposure increased GSIS, adding metformin after 0.5 days reduced GSIS. In children with “reducing” insulin AUC0–120 (n = 9), 2 h glucose and triglycerides decreased after metformin treatment, which was not observed in patients with “increasing” insulin AUC0–120 (n = 12). In isolated islets, metformin attenuated insulin hypersecretion if introduced when islet secretory capacity was maintained. In children with obesity, improved glycemic and lipid levels were accompanied by reduced insulin levels during OGTT after metformin treatment.
Current Biomarkers for Carotid Artery Stenosis: A Comprehensive Review of the Literature
Authors: Khan Hamzah , Shaikh Farah , Syed Muzammil H. , Mamdani Muhammad , Saposnik Gustavo , Qadura Mohammad
Published: August 2023

Carotid artery stenosis (CAS), an atherosclerotic disease of the carotid artery, is one of the leading causes of transient ischemic attacks (TIA) and cerebrovascular attacks (CVA). The atherogenic process of CAS affects a wide range of physiological processes, such as inflammation, endothelial cell function, smooth muscle cell migration and many more. The current gold-standard test for CAS is Doppler ultrasound; however, there is yet to be determined a strong, clinically validated biomarker in the blood that can diagnose patients with CAS and/or predict adverse outcomes in such patients. In this comprehensive literature review, we evaluated all of the current research on plasma and serum proteins that are current contenders for biomarkers for CAS. In this literature review, 36 proteins found as potential biomarkers for CAS were categorized in to the following nine categories based on protein function: (1) Inflammation and Immunity, (2) Lipid Metabolism, (3) Haemostasis, (4) Cardiovascular Markers, (5) Markers of Kidney Function, (6) Bone Health, (7) Cellular Structure, (8) Growth Factors, and (9) Hormones. This literature review is the most up-to-date and current comprehensive review of research on biomarkers of CAS, and the only review that demonstrated the several pathways that contribute to the initiation and progression of the disease. With this review, future studies can determine if any new markers, or a panel of the proteins explored in this study, may be contenders as diagnostic or prognostic markers for CAS.
The Design of a Dynamic Configurable Packet Parser Based on FPGA
Authors: Sun Ying , Guo Zhichuan
Published: August 2023

To meet the evolving demands of programmable networks and address the limitations of traditional fixed-type protocol parsers, we propose a dynamic and configurable low-latency parser implemented on an FPGA. The architecture consists of three protocol analysis modules and a TCAM-SRAM. Latency is reduced by optimizing the state machine and parallel extraction matching. At the same time, we introduce the chain mapping idea and container concept to formulate the matching and extraction rules of table entries and enhance the extensibility of the parser. Furthermore, our system supports dynamic configuration through SDN control, allowing flexible adaptation to diverse scenarios. Our design has been verified and simulated with a cocotb-based framework. The resulting architecture is implemented on Xilinx Ultrascale+ FPGAs and supports a throughput of more than 80 Gbps, with a maximum latency of only 36 nanoseconds for L4 protocol parsing.
Crop Productivity Boosters: Native Mycorrhizal Fungi from an Old-Growth Grassland Benefits Tomato (Solanum lycopersicum) and Pepper (Capsicum annuum) Varieties in Organically Farmed Soils
Authors: Koziol Liz , Bever James D.
Published: August 2023

This paper investigates the response of five tomato and five pepper varieties to native arbuscular mycorrhizal (AM) fungal inoculation in an organic farming system. The field experiment was conducted across a growing season at a working organic farm in Lawrence, KS, USA. The researchers hypothesized that native AM fungi inoculation would improve crop biomass production for both crop species, but that the magnitude of response would depend on crop cultivar. The results showed that both crops were significantly positively affected by inoculation. AM fungal inoculation consistently improved total pepper biomass throughout the experiment (range of +2% to +8% depending on the harvest date), with a +3.7% improvement at the final harvest for inoculated plants. An interaction between pepper variety and inoculation treatment was sometimes observed, indicating that some pepper varieties were more responsive to AM fungi than others. Beginning at the first harvest, tomatoes showed a consistent positive response to AM fungal inoculation among varieties. Across the experiment, AM fungi-inoculated tomatoes had +10% greater fruit biomass, which was driven by a +20% increase in fruit number. The study highlights the potential benefits of using native AM fungi as a soil amendment in organic farmed soils to improve pepper and tomato productivity.
Infection, Allergy, and Inflammation: The Role of Aspergillus fumigatus in Cystic Fibrosis
Authors: Poore T. Spencer , Zemanick Edith T.
Published: August 2023

Aspergillus fumigatus (Af) is a mold frequently detected in airway samples from people with cystic fibrosis (pwCF). Abnormal airway mucus may allow Af to germinate, resulting in airway infection or an allergic response. While Af is known to increase morbidity in pwCF, individual responses and the degree of impact on lung disease vary. Improved approaches to diagnosis, treatment, and prevention of Af, particularly the persistent Af infection, are needed. This update highlights our current understanding of Af pathophysiology in the CF airway, the effects of Af on pwCF, and areas of research needed to improve clinical outcomes.
A bvrR/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR/BvrS in Virulence and Membrane Integrity
Authors: Rivas-Solano Olga , Núñez-Montero Kattia , Altamirano-Silva Pamela , Ruiz-Villalobos Nazareth , Barquero-Calvo Elías , Moreno Edgardo , Chaves-Olarte Esteban , Guzmán-Verri Caterina
Published: August 2023

Brucella abortus is a bacterial pathogen causing bovine brucellosis worldwide. This facultative extracellular–intracellular pathogen can be transmitted to humans, leading to a zoonotic disease. The disease remains a public health concern, particularly in regions where livestock farming is present. The two-component regulatory system BvrR/BvrS was described by isolating the attenuated transposition mutants bvrR::Tn5 and bvrS::Tn5, whose characterization led to the understanding of the role of the system in bacterial survival. However, a phenotypic comparison with deletion mutants has not been performed because their construction has been unsuccessful in brucellae and difficult in phylogenetically related Rhizobiales with BvrR/BvrS orthologs. Here, we used an unmarked gene excision strategy to generate a B. abortus mutant strain lacking both genes, called B. abortus ∆bvrRS. The deletion was verified through PCR, Southern blot, Western blot, Sanger sequencing, and whole-genome sequencing, confirming a clean mutation without further alterations at the genome level. B. abortus ∆bvrRS shared attenuated phenotypic traits with both transposition mutants, confirming the role of BvrR/BvrS in pathogenesis and membrane integrity. This B. abortus ∆bvrRS with a non-antimicrobial marker is an excellent tool for continuing studies on the role of BvrR/BvrS in the B. abortus lifestyle.
Autophagy Alters the Susceptibility of Candida albicans Biofilms to Antifungal Agents
Authors: Shen Jiadi , Ma Ming , Duan Wei , Huang Yun , Shi Banruo , Wu Qiaochu , Wei Xin
Published: August 2023

Candida albicans (C. albicans) reigns as a major cause of clinical candidiasis. C. albicans biofilms are known to increase resistance to antifungal agents, making biofilm-related infections particularly challenging to treat. Drug resistance is of particular concern due to the spread of multidrug-resistant fungal pathogens, while autophagy is crucial for the maintenance of cellular homeostasis. Therefore, this study aimed to investigate the effects of an activator and an inhibitor of autophagy on the susceptibility of C. albicans biofilms to antifungal agents and the related mechanisms. The susceptibility of C. albicans biofilms to different antifungal agents after treatment with or without the autophagy activator or inhibitor was evaluated using XTT assay. Alkaline phosphatase (ALP) activity and reactive oxygen species (ROS) level, as well as the expression of ROS-related and autophagy-related genes, were examined to evaluate the autophagic activity of C. albicans biofilms when treated with antifungal agents. The autophagosomes were observed by transmission electron microscopy (TEM). The susceptibility of C. albicans biofilms to antifungal agents changed when autophagy changed. The ALP activity and ROS level of C. albicans biofilms increased with the treatment of antifungal agents, and autophagosomes could be observed in C. albicans biofilms. Autophagy was involved in the susceptibility of C. albicans biofilms to antifungal agents.
Predicting Adverse Recanalization Therapy Outcomes in Acute Ischemic Stroke Patients Using Characteristic Gut Microbiota
Authors: Chou Ping-Song , Hung Wei-Chun , Yang I-Hsiao , Kuo Chia-Ming , Wu Meng-Ni , Lin Tzu-Chao , Fong Yi-On , Juan Chi-Hung , Lai Chiou-Lian
Published: August 2023

Recanalization therapy is the most effective treatment for eligible patients with acute ischemic stroke (AIS). Gut microbiota are involved in the pathological mechanisms and outcomes of AIS. However, the association of gut microbiota features with adverse recanalization therapy outcomes remains unclear. Herein, we investigated gut microbiota features associated with neurological deficits in patients with AIS after recanalization therapy and whether they predict the patients’ functional outcomes. We collected fecal samples from 51 patients with AIS who received recanalization therapy and performed 16S rRNA gene sequencing (V3–V4). We compared the gut microbiota diversity and community composition between mild to moderate and severe disability groups. Next, the characteristic gut microbiota was compared between groups, and we noted that the characteristic gut microbiota in patients with mild to moderate disability included Bilophila, Butyricimonas, Oscillospiraceae_UCG-003, and Megamonas. Moreover, the relative abundance of Bacteroides fragilis, Fusobacterium sp., and Parabacteroides gordonii was high in patients with severe disability. The characteristic gut microbiota was correlated with neurological deficits, and areas under the receiver operating characteristic curves confirmed that the characteristic microbiota predicted adverse recanalization therapy outcomes. In conclusion, gut microbiota characteristics are correlated with recanalization therapy outcomes in patients with AIS. Gut microbiota may thus be a promising biomarker associated with early neurological deficits and predict recanalization therapy outcomes.
The Role and Mechanism of Perilla frutescens in Cancer Treatment
Authors: Huang Shicong , Nan Yi , Chen Guoqing , Ning Na , Du Yuhua , Lu Doudou , Yang Yating , Meng Fandi , Yuan Ling
Published: August 2023

Perilla frutescens is an annual herb of the Labiatae family and is widely grown in several countries in Asia. Perilla frutescens is a plant that is used medicinally in its entirety, as seen in its subdivision into perilla seeds, perilla stalks, and perilla leaves, which vary more markedly in their chemical composition. Several studies have shown that Perilla frutescens has a variety of pharmacological effects, including anti-inflammatory, antibacterial, detoxifying, antioxidant, and hepatoprotective. In the absence of a review of Perilla frutescens for the treatment of cancer. This review provides an overview of the chemical composition and molecular mechanisms of Perilla frutescens for cancer treatment. It was found that the main active components of Perilla frutescens producing cancer therapeutic effects were perilla aldehyde (PAH), rosmarinic acid (Ros A), lignan, and isoestrogen (IK). In addition to these, extracts of the leaves and fruits of Perilla frutescens are also included. Among these, perilla seed oil (PSO) has a preventive effect against colorectal cancer due to the presence of omega-3 polyunsaturated fatty acids. This review also provides new ideas and thoughts for scientific innovation and clinical applications related to Perilla frutescens.
Zn-Induced Synthesis of Porous Fe-N,S-C Electrocatalyst with Iron-Based Active Sites Containing Sulfides, Oxides and Nitrides for Efficient Oxygen Reduction and Zinc-Air Batteries
Authors: Zhao Haiyan , Chen Li , Ni Nan , Lv Yang , Wang Hezhen , Zhang Jia , Li Zhiwen , Liu Yu , Geng Yubo , Xie Yan , Wang Li
Published: August 2023

There is an urgent need to design and synthesize non-noble metal electrocatalysts (NNMEs) for the replacement of platinum-based electrocatalysts to enhance the sluggish oxygen reduction reaction (ORR) for Zn–air batteries and fuel cells. Herein, Fe-N,S-C materials were fabricated through two steps: first, reprecipitating hemin by adjusting the pH and, then, decorating it with melamine and cysteine in the presence of Zn2+. The resulting Fe-N,S-C-950 (Zn) was prepared after pyrolysis at 950 °C. Using this method, abundant iron-based active species with good dispersion were obtained. The fabrication of more micropores in Fe-N,S-C-950 (Zn) plays a positive role in the improvement of ORR activity. On comparison, Fe-N,S-C-950 (Zn) outperforms Fe-N,S-C-950 and Fe-N-C-950 (Zn) with respect to the ORR due to its larger specific surface area, porous structure, multiple iron-based active sites and N- and S-doped C. Fe-N,S-C-950 (Zn) achieves outstanding ORR performances, including a half-wave potential (E1/2) of 0.844 V and 0.715 V versus a reversible hydrogen electrode (RHE) in 0.1 M KOH and 0.1 M HClO4 solution, respectively. In addition, Fe-N,S-C-950 (Zn) shows an outstanding Zn–air battery performance with an open-circuit voltage (OCV) of 1.450 V and a peak power density of 121.9 mW cm−2, which is higher than that of 20 wt% Pt/C. As a result, the as-prepared electrocatalyst in this work shows the development of the Zn-assisted strategy combined with the assembly of porphyrins as NNMEs for the enhancement of the ORR in both alkaline and acidic solutions.
Unraveling the Role of Natural Sediments in sII Mixed Gas Hydrate Formation: An Experimental Study
Authors: Pan Mengdi , Schicks Judith M.
Published: August 2023

Considering the ever-increasing interests in natural gas hydrates, a better and more precise knowledge of how host sediments interact with hydrates and affect the formation process is crucial. Yet less is reported for the effects of sediments on structure II hydrate formation with complex guest compositions. In this study, experimental simulations were performed based on the natural reservoir in Qilian Mountain permafrost in China (QMP) due to its unique properties. Mixed gas hydrates containing CH4, C2H6, C3H8, and CO2 were synthesized with the presence of natural sediments from QMP, with quartz sands, and without sediments under identical p–T conditions. The promoting effects of sediments regardless of the grain size and species were confirmed on hydrate formation kinetics. The ice-to-hydrate conversion rate with quartz sand and natural QMP sediments increased by 23.5% and 32.7%, respectively. The compositions of the initial hydrate phase varied, but the difference became smaller in the resulting hydrate phases, having reached a steady state. Beside the structure II hydrate phase, another coexisting solid phase, neither ice nor structure I hydrate, was observed in the system with QMP sediments, which was inferred as an amorphous hydrate phase. These findings are essential to understand the mixed gas hydrates in QMP and may shed light on other natural hydrate reservoirs with complex gas compositions.
Fabrication and Biological Activities of All-in-One Composite Nanoemulsion Based on Blumea balsamifera Oil-Tea Tree Oil
Authors: Zhu Yue , Chen Teng , Feng Tingting , Zhang Jiaojiao , Meng Zejing , Zhang Ning , Luo Gang , Wang Zuhua , Pang Yuxin , Zhou Ying
Published: August 2023

Nanoemulsion is a new multi-component drug delivery system; the selection of different oil phases can give it special physiological activity, and play the role of “medicine and pharmaceutical excipients all-in-one”. In this paper, we used glycyrrhizic acid as the natural surfactant, and Blumea balsamifera oil (BB) and tea tree oil (TTO) as the mixed oil phase, to obtain a new green functional composite nanoemulsion. Using the average particle size and polydispersion index (PDI) as the evaluation criteria, the effects of the oil ratio, oil content, glycyrrhizic acid concentration, and ultrasonic time on the nanoemulsion were systematically investigated. The stability and physicochemical properties and biological activities of BB-TTO NEs prepared via the optimum formulation were characterized. The optimal prescription was BB: TTO = 1:1, 5% oil phase, 0.7% glycyrrhizic acid, and 5 min ultrasonication time. The mean particle size, PDI, and zeta potential were 160.01 nm, 0.125, and −50.94 mV, respectively. The nanoemulsion showed non-significant changes in stability after centrifugation, dilution, and 120 days storage. These nanoemulsions were found to exhibit potential antibacterial and anti-inflammatory activities. The minimal inhibitory concentration (MIC) of BB-TTO NEs against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa is 2975 μg/mL, 2975 μg/mL, and 5950 μg/mL, respectively. A lower level of inflammatory cell infiltration and proportion of fibrosis were found in the synovial tissue of AIA rats treated with BB-TTO NEs. These findings demonstrate that the BB-TTO NEs produced in this study have significant potential for usage in antibacterial and anti-inflammatory areas.
Identification of Salivary Metabolic Signatures Associated with Primary Sjögren’s Disease
Authors: Alt-Holland Addy , Huang Xuejian , Mendez Tatiana , Singh Mabi L. , Papas Athena S. , Cimmino Joseph , Bairos Tiffany , Tzavaras Elizabeth , Foley Elizabeth , Pagni Sarah E. , Baleja James D.
Published: August 2023

Sjögren’s disease (SjD) is the second most prevalent autoimmune disorder that involves chronic inflammation of exocrine glands. Correct diagnosis of primary SjD (pSjD) can span over many years since disease symptoms manifest only in advanced stages of salivary and lachrymal glandular destruction, and consensus diagnostic methods have critical sensitivity and selectivity limitations. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the composition of metabolites in unstimulated saliva samples from 30 pSjD subjects and 30 participants who do not have Sjögren’s disease (non-Sjögren’s control group, NS-C). Thirty-four metabolites were quantified in each sample, and analysis was conducted on both non-normalized (concentration) and normalized metabolomics data from all study participants (ages 23–78) and on an age-restricted subset of the data (ages 30–70) while applying false discovery rate correction in determining data significance. The normalized data of saliva samples from all study participants, and of the age-restricted subset, indicated significant increases in the levels of glucose, glycerol, taurine, and lactate, as well as significant decreases in the levels of 5-aminopentanoate, acetate, butyrate and propionate, in subjects with pSjD compared to subjects in the NS-C group. Additionally, a significant increase in choline was found only in the age-restricted subset, and a significant decrease in fucose was found only in the whole study population in normalized data of saliva samples from the pSjD group compared to the NS-C group. Metabolite concentration data of saliva samples from all study participants, but not from the age-restricted subset, indicated significant increases in the levels of glucose, glycerol, taurine, and lactate in subjects with pSjD compared to controls. The study showed that NMR metabolomics can be implemented in defining salivary metabolic signatures that are associated with disease status, and can contribute to differential analysis between subjects with pSjD and those who are not affected with this disease, in the clinic.
Influence of Ligand Environment Stoichiometry on NIR-Luminescence Efficiency of Sm3+, Pr3+ and Nd3+ Ions Coordination Compounds
Authors: Polikovskiy Trofim , Korshunov Vladislav , Metlin Mikhail , Gontcharenko Viktoria , Metlina Darya , Datskevich Nikolay , Kiskin Mikhail , Belousov Yury , Tsorieva Alisia , Taydakov Ilya
Published: August 2023

Six new complexes of the ligand HQcy (-4-(cyclohexanecarbonyl)-5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one) and Ln3+ ions with emission in the near-infrared (Nd3+) or visible and near-infrared (Sm3+, Pr3+) spectral regions were synthesized and characterized using various methods, including single crystal X-ray diffraction. The study demonstrated that both tris complexes [LnQcy3(H2O)(EtOH)] and tetrakis-acids [H3O][LnQcy4] can be synthesized by varying the synthetic conditions. The photochemical properties of the complexes were investigated experimentally and theoretically using various molecular spectroscopy techniques and Judd–Ofelt theory. The objective was to quantitatively and qualitatively disclose the influence of complex stoichiometry on its luminescence properties. The study showed that the addition of an extra ligand molecule (in the tetrakis species) increased molar extinction by up to 2 times, affected the shape of photoluminescence spectra, especially of the Pr3+ complex, and increased the quantum yield of the Sm3+ complex by up to 2 times. The results obtained from this study provide insights into the luminescent properties of lanthanide coordination compounds, which are crucial for the design and development of novel photonic materials with tailored photophysical properties.
Oxide Materials for Thermoelectric Conversion
Authors: Liu Yucen , Zhi Jun , Li Wannuo , Yang Qian , Zhang Long , Zhang Yuqiao
Published: August 2023

Thermoelectric technology has emerged as a prominent area of research in the past few decades for harnessing waste heat and improving the efficiency of next-generation renewable energy technologies. There has been rapid progress in the development of high-performance thermoelectric materials, as measured by the dimensionless figure of merit (ZT = S2 · σ · κ−1). Several heavy-metal-based thermoelectric materials with commercial-level performance (ZT = 1) have so far been proposed. However, the extensive application of these materials still faces challenges due to their low thermal/chemical stability, high toxicity, and limited abundance in the Earth’s crust. In contrast, oxide-based thermoelectric materials, such as ZnO, SrTiO3, layered cobalt oxides, etc., have attracted growing interest as they can overcome the limitations of their heavy-metal-based counterparts. In this review, we summarize the recent research progress and introduce improvement strategies in oxide-based thermoelectric materials. This will provide an overview of their development history and design schemes, ultimately aiding in enhancing the overall performance of oxide-based thermoelectric materials.
Synthesis and Characterization of Methoxy-Exfoliated Montmorillonite Nanosheets as Potential Carriers of 5-Fluorouracil Drug with Enhanced Loading, Release, and Cytotoxicity Properties
Authors: Alqahtani Mashael D. , Bin Jumah May N. , Al-Hashimi Abdulrahman , Allam Ahmed A. , Abukhadra Mostafa R. , Bellucci Stefano
Published: August 2023

Natural bentonite clay (BE) underwent modification steps that involved the exfoliation of its layers into separated nanosheets (EXBE) and further functionalization of these sheets with methanol, forming methoxy-exfoliated bentonite (Mth/EXBE). The synthetically modified products were investigated as enhanced carriers of 5-fluorouracil as compared to raw bentonite. The modification process strongly induced loading properties that increased to 214.4 mg/g (EXBE) and 282.6 mg/g (Mth/EXBE) instead of 124.9 mg/g for bentonite. The loading behaviors were illustrated based on the kinetic (pseudo-first-order model), classic isotherm (Langmuir model), and advanced isotherm modeling (monolayer model of one energy). The Mth/EBE carrier displays significantly higher loading site density (95.9 mg/g) as compared to EXBE (66.2 mg/g) and BE (44.9 mg/g). The loading numbers of 5-Fu in each site of BE, EXBE, and Mth/EXBE (>1) reflect the vertical orientation of these loaded ions involving multi-molecular processes. The loading processes that occurred appeared to be controlled by complex physical and weak chemical mechanisms, considering both Gaussian energy (<8 KJ/mol) as well as loading energy (<40 KJ/mol). The releasing patterns of EXBE and Mth/EXBE exhibit prolonged and continuous properties up to 100 h, with Mth/EXBE displaying much faster behaviors. Based on the release kinetic modeling, the release reactions exhibit non-Fickian transport release properties, validating cooperative diffusion and erosion release mechanisms. The cytotoxicity of 5-Fu is also significantly enhanced by these carriers: 5-Fu/BE (8.6% cell viability), 5-Fu/EXBE (2.21% cell viability), and 5-Fu/Mth/EXBE (0.73% cell viability).