bluuebunny/crossref_metadata_2025 huggingface dataset:
Advantages of Plate Antennas over Loop Antennas for Circular Polarization—Its Application in Array Antenna with a Simplified Feed
Authors: Hirose Kazuhide , Hirose Mitsuki , Mita Shintaro , Fujita Takumi , Nakano Hisamatsu
Published: October 2024

We analyze square antennas with one and quasi-two sources to reveal the relationship between loop and plate antennas. First, using the moment method, one-source antennas with corner truncation are investigated versus antenna height above the ground plane. As the height increases, the CP wave bandwidth of the plate antenna increases 17% for a 3 dB axial ratio criterion, whereas the loop antenna’s bandwidth remains less than 3%. Next, we study quasi-two-source antennas without corner truncation versus antenna height. The plate antenna’s bandwidth is found to reach 30%, which is wider than that of a loop antenna by a factor of two. Last, the plate antenna with quasi-two sources is applied in an array antenna, whose properties are compared to those of the corresponding loop antenna array. We present experimental results to verify the simulated results.
Robust Adaptive Sliding Mode Control Using Stochastic Gradient Descent for Robot Arm Manipulator Trajectory Tracking
Authors: Silaa Mohammed Yousri , Barambones Oscar , Bencherif Aissa
Published: October 2024

This paper presents an innovative control strategy for robot arm manipulators, utilizing an adaptive sliding mode control with stochastic gradient descent (ASMCSGD). The ASMCSGD controller significant improvements in robustness, chattering elimination, and fast, precise trajectory tracking. Its performance is systematically compared with super twisting algorithm (STA) and conventional sliding mode control (SMC) controllers, all optimized using the grey wolf optimizer (GWO). Simulation results show that the ASMCSGD controller achieves root mean squared errors (RMSE) of 0.12758 for θ1 and 0.13387 for θ2. In comparison, the STA controller yields RMSE values of 0.1953 for θ1 and 0.1953 for θ2, while the SMC controller results in RMSE values of 0.24505 for θ1 and 0.29112 for θ2. Additionally, the ASMCSGD simplifies implementation, eliminates unwanted oscillations, and achieves superior tracking performance. These findings underscore the ASMCSGD’s effectiveness in enhancing trajectory tracking and reducing chattering, making it a promising approach for robust control in practical applications of robot arm manipulators.
A Zero-Watermarking Algorithm Based on Vortex-like Texture Feature Descriptors
Authors: Li Fan , Wang Zhongxun
Published: October 2024

For effective copyright protection of digital images, this paper proposes a zero-watermarking algorithm based on local image feature information. The feature matrix of the algorithm is derived from the keypoint set determined by the Speeded-Up Robust Features (SURF) algorithm, and it calculates both the gradient feature descriptors and the vortex-like texture feature (VTF) descriptors of the keypoint set. Unlike traditional texture feature descriptors, the vortex-like texture feature descriptors proposed in this paper contain richer information and exhibit better stability. The advantage of this algorithm lies in its ability to calculate the keypoints of the digital image and provide a stable vector description of the local features of these keypoints, thereby reducing the amount of erroneous information introduced during attacks. Analysis of experimental data shows that the algorithm has good effectiveness, distinguishability, security, and robustness.
The Generation Rights Trading between Self-Owned Power Plants and New Energy Enterprises under the Conditions of Price Difference and Time-of-Use Pricing Settlement
Authors: Li Wei , Cheng Xiaolei , Gong Yuying , Qu Kaibo , Udabala  , Liu Jichun , Yu Xiang
Published: October 2024

Currently, the proportion of enterprise self-owned power plants (SPPs) is increasing, with a significant share occupied by small coal-fired units, severely affecting the absorption of new energy and causing substantial pollution. To address this issue, developing generation rights trading between SPPs and new energy enterprises is an effective solution. At present, research on generation rights trading is mainly based on early water and thermal generation rights replacement trading. This approach, to some extent, overlooks changes in electricity market policies. Based on this, a new generation rights trading bidding strategy incorporating price differences and time-of-use pricing settlement is proposed. Firstly, the relationship between price difference settlement and generation rights trading is studied and the win–win model of generation rights trading is improved. Secondly, in the time-of-use pricing settlement mode, the single bidding strategy is optimized with the objective of maximizing the total social benefits in the win–win model. Finally, an example analysis compares different bidding strategies under time-of-use pricing settlement. Even in the most extreme cases, the time-of-use bidding strategy can improve social benefits by 5.61% and reduce wind and solar curtailment by 7.25% compared to the single bid strategy. The results show that the optimized time-of-use bidding strategy significantly improves the efficiency of generation rights trading, greatly helping to promote the absorption of new energy and alleviate wind and solar power curtailment.
Bias Mitigation via Synthetic Data Generation: A Review
Authors: Shahul Hameed Mohamed Ashik , Qureshi Asifa Mehmood , Kaushik Abhishek
Published: October 2024

Artificial intelligence (AI) is widely used in healthcare applications to perform various tasks. Although these models have great potential to improve the healthcare system, they have also raised significant ethical concerns, including biases that increase the risk of health disparities in medical applications. The under-representation of a specific group can lead to bias in the datasets that are being replicated in the AI models. These disadvantaged groups are disproportionately affected by bias because they may have less accurate algorithmic forecasts or underestimate the need for treatment. One solution to eliminate bias is to use synthetic samples or artificially generated data to balance datasets. Therefore, the purpose of this study is to review and evaluate how synthetic data can be generated and used to mitigate biases, specifically focusing on the medical domain. We explored high-quality peer-reviewed articles that were focused on synthetic data generation to eliminate bias. These studies were selected based on our defined inclusion criteria and exclusion criteria and the quality of the content. The findings reveal that generated synthetic data can help improve accuracy, precision, and fairness. However, the effectiveness of synthetic data is closely dependent on the quality of the data generation process and the initial datasets used. The study also highlights the need for continuous improvement in synthetic data generation techniques and the importance of evaluation metrics for fairness in AI models.
A Study on the Energy Efficiency of an Energy Management System for Convenience Stores
Authors: Thomyapitak Thitiporn , Saengsikhiao Piyanat , Vessakosol Passakorn , Taweekun Juntakan
Published: October 2024

This research presents a solution for improving energy efficiency in convenience stores by implementing a building energy management system (BEMS) that uses new logic control in air conditioning and refrigeration systems. These systems currently consume the most energy in convenience stores. Implementing this system not only reduces the energy consumption of the compressors in both systems but also minimizes energy loss due to low desired temperatures in the sale area while maintaining the cabinet temperature at the same level. An experiment was conducted at a 314-square-meter convenience store that was open from 6:00 a.m. to 11:00 p.m., and we demonstrated a 4.4-year payback period by controlling AC units close to the desired sale-area temperature of 25 degrees Celsius and increasing the suction pressure at a medium-temperature CDU by 0.3 bar or 31 kPa. This resulted in energy savings of 7.1 kilowatt-hours per day, or 2591.5 kilowatt-hours per year, for the air conditioning system and 2.8 kilowatt-hours per day, or 1022.0 kilowatt-hours per year, for the refrigeration system, resulting in a total energy savings of 9.9 kilowatt-hours per day, or 3613.5 kilowatt-hours per year. The convenience store can use the results of this research to improve the energy efficiency of its cooling system, which includes air conditioning and refrigeration systems, thereby promoting sustainable energy conservation.
Farmers’ Willingness to Accept Afforestation in Farming Land and Its Influencing Factors in Fragile Landscapes Based on the Contingent Valuation Method
Authors: Karki Sharada , Yokota Shigehiro
Published: October 2024

Afforestation (AF) in farmland has been widely used as an alternative and sustainable land-use practice to address socioeconomic and environmental challenges. The aim of this study is to estimate farmers’ willingness to accept (WTA) compensation and land, both of which are equally significant for policymakers to ensure the effective implementation of AF and achieve desired outcomes. This topic has not been sufficiently explored in previous research. This study focused on areas characterized by insecure farming conditions, backward economies, and fragile landscapes, where farmers are generally unfamiliar with AF or compensation for ecosystem services under payment for ecosystem services programs. It assessed their attitudes towards the WTA AF, compensation, and land as an alternative practice, which remains under-researched. This is crucial for designing effective AF programs in the future to improve livelihood and enhance the quantity and quality of the environment. This study used the contingent valuation method to estimate the minimum WTA compensation and maximum land for the forgone loss and alternative land-use practices. A questionnaire survey was conducted in Hupsekot municipality, Nepal, with 232 farmer households. The ordinal logistic regression model was used to analyze influencing factors of WTA compensation and land. The result showed that farmers’ average WTA compensation was NPR 1268.67 (USD 9.76)/Kattha/year, with 2.64 Kattha land available for AF. The factors, including socioeconomic characters and attitudes toward the environmental situation and forests, significantly influenced WTA values and provided potential target factors to achieve maximum AF land within a lower budget.
Arc Ignition Methods and Combustion Characteristics of Small-Current Arc Faults in High-Voltage Cables
Authors: Guo Wei , Cai Jing , Ji Hongquan , Li Huachun , Ren Zhigang , Men Yekun , Pan Zehua
Published: October 2024

High-voltage cables will continue to operate for a period of time in the event of a small current arc fault, which poses a risk of fire. Two simulated ignition methods, moving electrode and melting fuses, are proposed to analyze the ignition characteristics of low-current arcs. The ignition test was carried out, and the combustion effect was compared. The results indicate that the moving electrode ignition method can achieve long-distance arc ignition test when the current is small and is suitable for simulating the arc ignition situation of cable outer protective layer damage. By controlling the movement speed, it can be ensured that the arc will not be interrupted during the electrode movement process. However, the arc is difficult to sustain using the fuse melting method when the current is small and the distance is long. The fuse melting method is suitable for simulating insulation breakdown situations. The results show that the critical arc duration for cable ignition under five different current conditions of 2–10 A is 28 s, 21 s, 14 s, 9 s, and 4 s, respectively. The maximum height of the cable flame under 2–10 A arc current is 9–52 cm and 16–63 cm, respectively, when the arc duration is 50 s and 100 s. The self-ignition time of the cable after the arc extinguishing is 8–95 s and 14–261 s, respectively. The maximum temperature of the cable flame is positively correlated with arc current, and the maximum flame temperature of the cable under 2–10 A arc current is 540–980 °C. Based on the actual current monitoring data in cable tunnels, the research results can provide reference for the risk assessment and protection of cable tunnel fires.
Effects of Risk and Time Preferences on Diet Quality: Empirical Evidence from Rural Madagascar
Authors: Shiratori Sakiko , Abeysekara Mudduwa Gamaethige Dilini , Ozaki Ryosuke , Rafalimanantsoa Jules , Rasolonirina Andrianjanaka Britney Havannah
Published: October 2024

Malnutrition is a major concern in Madagascar. Eating a wide variety of nutritious food is necessary because Malagasy diets heavily rely on rice consumption. This study explored the barriers to dietary change towards diversification from the perspective of consumer behaviour. We analysed the impact of risk and time preferences on dietary diversity using economic experiments conducted with 539 rural lowland rice farmers in Central Highlands in Madagascar. The results showed that risk-averse or impatient individuals were more likely to have lower Household Dietary Diversity Score (HDDS), indicating poorer diet quality. Risk-averse people may not want to add different foods to meals as they perceive unfamiliar food as a ‘risk’; people who prefer immediate gratification may fail to invest in nutritious diets now to achieve better health in the future. Additionally, higher HDDS was observed among households with a female head who earned off-farm income and who had frequent market visits. These findings contribute to explaining the limited shift in nutritional transition in Madagascar and provide useful insights into nutritional policies promoting healthier food choices. Depending on the preferences, more focused support such as nutritional education, financial support, market development, and pre-commitment mechanisms could be provided to reward long-term nutritional benefits.
Against the Grain: Consumer’s Purchase Habits and Satisfaction with Gluten-Free Product Offerings in European Food Retail
Authors: Dean David , Rombach Meike , Vriesekoop Frank , Mongondry Philippe , Le Viet Hoa , Laophetsakunchai Sirasit , Urbano Beatriz , Briz Teresa , Xhakollari Vilma , Atasoy Güler , Turhan Mahir , Chrysostomou Stavroula , Hadjimbei Elena , Hassan Hussein , Bassil Maya , Arnala Sanna , Głąbska Dominika , Guzek Dominika , van den Berg Sophie , Ossel Lilian , Scannell Amalia , Rauniyar Puja , Bathrellou Eirini , Kontogianni Meropi , de Koning Wim
Published: October 2024

Across the world and within Europe, a growing number of consumers are choosing to buy gluten-free products. Motivations for a gluten-free diet and the consequences of consuming gluten are varied, from a medical necessity for those diagnosed with celiac disease to a range of health complications and discomfort for those who are gluten-intolerant. In this research, 7296 gluten-free consumers across 13 European countries responded to an online survey on the 33 types of gluten-free products purchased, how frequently they purchased them, their satisfaction with gluten-free quality and availability, the problems they have experienced, and the strategies they have employed to cope with these problems. The investigation examines whether and how these consumer attitudes and behaviors differ between those diagnosed with celiac disease, those who are gluten-intolerant, and those who are caregivers for others with a gluten-free diet. The results show that significant differences existed for all these habits and issues across the three gluten-free consumer groups. Specifically, caregivers purchased most of the gluten-free product types more frequently than the other two groups, experienced more availability problems, and were more likely to shop at multiple stores or make their own gluten-free products. Celiac-diagnosed consumers tended to buy gluten-free products more frequently than those who are gluten-intolerant, and they tended to be the most satisfied with the quality and range of gluten-free offerings. Despite purchasing frequency differences between the groups, the results suggest a similar hierarchy of gluten-free products that could provide the foundation for a European gluten-free food basket.
Arsenic in Rice and Rice-Based Products with Regard to Consumer Health
Authors: Rajkowska-Myśliwiec Monika , Ciemniak Artur , Karp Gabriela
Published: October 2024

Most articles on the exposure to arsenic (As) associated with rice and rice products come from Asia where these products are consumed in the largest quantities; relatively few of the articles have focused on European consumers. Since rice products can represent a significant contribution to overall arsenic exposure, the aim of the study was to determine the total arsenic content (tAs) in rice and the most commonly-consumed rice products available on the Polish market. The tAs determination was performed by hydride generation coupled to inductively-coupled plasma optical emission spectrometry (HG-ICP-OES). Because an inorganic form of As (iAs) is mutagenic and carcinogenic and about 100-fold more toxic than the organic form, an additional aim of the study was to assess the risk of its ingestion, assuming that it constitutes 67.7%, 72.7%, or 90% of tAs. In all products tested, the calculated iAs content was below the maximum permissible levels, and no threat was found for any of the analyzed Polish consumers, based on the mean rice consumption in Poland and the mean calculated iAs content. However, a potential health risk was noted among infants and young children, assuming maximum iAs levels and threefold higher consumption (16.2 g d−1). To avoid a risk of developing cancer, infants up to one year of age should consume no more than 32.2 g of the studied products per week, children under three years of age up to 68.7 g, and adults 243 g. Consumers should strive to include a variety of cereals in their daily diet and choose products shown to have low arsenic contamination levels based on testing and inspection rankings.
Strengthening Health Security at Ground Border Crossings: Key Components for Improved Emergency Preparedness and Response—A Scoping Review
Authors: Sami Diana G. , Chun Sungsoo
Published: October 2024

Background: Ground crossing borders are considered a threat to health security due to their complex and challenging environments. The objective of this scoping review is to identify the main themes crucial for implementing effective health management at ground crossing borders to maintain health security. Methods: This scoping study was conducted following the methodological framework developed by Arksey and O’Malley and the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Documents published from January 2005 to December 2023 were searched for using PubMed, Scopus, Web of Science, and UN databases. Two reviewers screened and reviewed eligible studies in three stages: duplicate identification and elimination, title and abstract screening, and full-text assessment. Data were charted and grouped into themes, the frequency of each theme and its percentage was calculated, and then thematic analysis was conducted. Results: Forty-five studies met the inclusion criteria. Areas of research were grouped into eight themes: “Infection Prevention and Control measures (IPC) (17%)”, “Collaboration, Coordination, and Partnership (17%)”, “Research and Data Sharing (15%)”, “Build Border Health Capacity (13.5%)”, “Planning (13.5%)”, “Communication (13%)”, “Legislations and Frameworks (7%)”, and “Services and Assistance for At-Risk Groups (4%)”. Through this scoping review, we found that the eight themes are interconnected, and are crucial for implementing effective health management at the ground crossing borders and for better emergency preparedness responses among countries.
Identifying Fire Blight-Resistant Malus sieversii Rootstocks Grafted with Cultivar ‘Aport’ Using Monitoring Data
Authors: Taskuzhina Aisha , Pozharskiy Alexandr , Jumanova Zhulduzay , Soltanbekov Sagi , Issina Zhanna , Kerimbek Nazym , Kapytina Anastasiya , Khusnitdinova Marina , Sagitov Abay , Darubayev Alibi , Seisenova Aigerim , Omarov Yerlan , Gritsenko Dilyara
Published: October 2024

In the present study, the most valuable cultivar ‘Aport krovavo-krasnyy’ was grafted onto M. sieversii genotypes harvested from 11 populations in Dzungarian Alatau and Ile Alatau to identify ones resistant to Erwinia amylovora. The wild apple populations included in the present research have not been previously explored. Seedling population 10, developed using rootstocks from a M. sieversii population growing in Turgen, demonstrated the highest resistance to Erwinia amylovora, showing no fire blight symptoms and no positive PCR results for E. amylovora during the eight years of monitoring in the Talgar field (Kazakhstan) from 2015 to 2022. The population from Steep Tract (seedling population 1) was also valuable for breeding and reduced the pathogen distribution to below 30%. Genotypes from a genetic reserve (seedling population 5) were the most susceptible among the researched populations, with a disease distribution level of 24–95%. In seedling population 5, trees affected at least twice by the pathogen exhibited wilting, shepherd’s crook formation, leaf necrosis, and occasional exudate droplets, while trees in other combinations primarily showed shoot wilting and leaf death. Fire blight disease also developed more rapidly within the plant in seedling population 5; by 2020, one tree nearly died after only two infections.
Latent Tuberculosis in Healthcare Professionals: A Cross-Sectional Study
Authors: Garzón Prados Marta , Jiménez Sampelayo Laura , Tovar-Gálvez María Isabel , Sánchez-García Juan Carlos , Cortés-Martín Jonathan , Piqueras-Sola Beatriz , Rodríguez-Blanque Raquel
Published: October 2024

Tuberculosis (TB) is a dangerous disease caused by the bacterium Mycobacterium tuberculosis. Its transmission occurs through aerosols, making healthcare workers particularly vulnerable to it. At the San Cecilio Clinical University Hospital in Granada (HUCSC), preventive measures such as the use of personal protective equipment (PPE) and IGRA (interferon-gamma release assay) tests were implemented to detect latent tuberculosis infection. At HUCSC, between 2022 and 2024, 4.75% (92/1936) of workers were found to have positive IGRA test results, with no significant differences observed between professional categories. However, variability in positive IGRA rates was noted across different medical departments, with pulmonology and sample handling being the most affected. Additionally, a relationship was found between age and the likelihood of obtaining a positive IGRA test result. These findings underscore the importance of targeted preventive measures and a multifaceted approach to controlling and preventing tuberculosis in workplace environments.
HNF1B Transcription Factor: Key Regulator in Renal Physiology and Pathogenesis
Authors: Sánchez-Cazorla Eloísa , Carrera Noa , García-González Miguel Ángel
Published: October 2024

The HNF1B gene, located on chromosome 17q12, encodes a transcription factor essential for the development of several organs. It regulates the expression of multiple genes in renal, pancreatic, hepatic, neurological, and genitourinary tissues during prenatal and postnatal development, influencing processes such as nephrogenesis, cellular polarity, tight junction formation, cilia development, ion transport in the renal tubule, and renal metabolism. Mutations that alter the function of Hnf1b deregulate those processes, leading to various pathologies characterized by both renal and extrarenal manifestations. The main renal diseases that develop are polycystic kidney disease, hypoplastic or dysplastic kidneys, structural abnormalities, Congenital Anomalies of the Kidney and Urinary Tract (CAKUT), and electrolyte imbalances such as hyperuricemia and hypomagnesemia. Extrarenal manifestations include Maturity-Onset Diabetes of the Young (MODY), hypertransaminasemia, genital and urinary tract malformations, Autism Spectrum Disorder (ASD), and other neurodevelopmental disorders. Patients with HNF1B alterations typically carry either punctual mutations or a monoallelic microdeletion in the 17q12 region. Future research on the molecular mechanisms and genotype–phenotype correlations in HNF1B-related conditions will enhance our understanding, leading to improved clinical management, genetic counseling, monitoring, and patient care.
TR3-56 and Treg Regulatory T Cell Subsets as Potential Indicators of Graft Tolerance Control in Kidney Transplant Recipients
Authors: Rubino Valentina , Carriero Flavia , Palatucci Anna Teresa , Giovazzino Angela , Salemi Fabrizio , Carrano Rosa , Sabbatini Massimo , Ruggiero Giuseppina , Terrazzano Giuseppe
Published: October 2024

Identification of early signatures of immune rejection represents a key challenge in the clinical management of kidney transplant. To address such an issue, we enrolled 53 kidney transplant recipients without signs of graft rejection, no infectious episodes and no change in the immunosuppressive regimen in the last 6 months. An extensive immune profile revealed increased activation of the T cells, a decreased amount and growth ability of the Treg and a higher level of the TR3-56 regulatory T cell subset, described by us as involved in the preferential control of cytotoxic T lymphocytes. In renal transplant recipients, the high level of the TR3-56 cells associates with a reduction in both the amount and the growth ability of the Treg. Moreover, when the transplanted subjects were categorised according to their stable or unstable disease status, as defined by changes in serum creatinine ≥0.2 mg/dL in two consecutive detections, a higher TR3-56 level and defective Treg growth ability were observed to characterise patients with unstable graft control. Further studies are required to substantiate the hypothesis that immune profiling, including TR3-56 evaluation, might represent a valuable diagnostic tool to identify patients at risk of developing significant anti-donor allo-immune responses.
Generation and Characterization of hiPS Lines from Three Patients Affected by Different Forms of HPDL-Related Neurological Disorders
Authors: Baggiani Matteo , Damiani Devid , Privitera Flavia , Della Vecchia Stefania , Tessa Alessandra , Santorelli Filippo Maria
Published: October 2024

Hereditary spastic paraplegias are rare genetic disorders characterized by corticospinal tract impairment. Spastic paraplegia 83 (SPG83) is associated with biallelic mutations in the HPDL gene, leading to varied severities from neonatal to juvenile onset. The function of HPDL is unclear, though it is speculated to play a role in alternative coenzyme Q10 biosynthesis. Here, we report the generation of hiPS lines from primary skin fibroblasts derived from three SPG83 patients with different HPDL mutations, using episomal reprogramming. The patients’ clinical characteristics are carefully listed. The hiPS lines were meticulously characterized, demonstrating typical pluripotent characteristics through immunofluorescence assays for stemness markers (OCT4, TRA1-60, NANOG, and SSEA4) and RT-PCR for endogenous gene expression. Genetic integrity and identity were confirmed via Sanger sequencing and short tandem repeat analysis. These hiPS cells displayed typical pluripotent characteristics and were able to differentiate into neocortical neurons via a dual SMAD inhibition protocol. In addition, HPDL mutant neurons assessed via long-term culturing were able to achieve effective maturation, similarly to their wild-type counterparts. The HPDL hiPS lines we generated will provide a valuable model for studying SPG83, offering insights into its molecular mechanisms and potential for developing targeted therapies.
Microfluidic Affinity Selection of B-Lineage Cells from Peripheral Blood for Minimal Residual Disease Monitoring in Pediatric B-Type Acute Lymphoblastic Leukemia Patients
Authors: Witek Malgorzata A. , Larkey Nicholas E. , Bartakova Alena , Hupert Mateusz L. , Mog Shalee , Cronin Jami K. , Vun Judy , August Keith J. , Soper Steven A.
Published: October 2024

Assessment of minimal residual disease (MRD) is the most powerful predictor of outcome in B-type acute lymphoblastic leukemia (B-ALL). MRD, defined as the presence of leukemic cells in the blood or bone marrow, is used for the evaluation of therapy efficacy. We report on a microfluidic-based MRD (MF-MRD) assay that allows for frequent evaluation of blood for the presence of circulating leukemia cells (CLCs). The microfluidic chip affinity selects B-lineage cells, including CLCs using anti-CD19 antibodies poised on the wall of the microfluidic chip. Affinity-selected cells are released from the capture surface and can be subjected to immunophenotyping to enumerate the CLCs, perform fluorescence in situ hybridization (FISH), and/or molecular analysis of the CLCs’ mRNA/gDNA. During longitudinal testing of 20 patients throughout induction and consolidation therapy, the MF-MRD performed 116 tests, while only 41 were completed with multiparameter flow cytometry (MFC-MRD) using a bone marrow aspirate, as standard-of-care. Overall, 57% MF-MRD tests were MRD(+) as defined by CLC numbers exceeding a threshold of 5 × 10−4%, which was determined to be the limit of quantitation. Above a threshold of 0.01%, MFC-MRD was positive in 34% of patients. The MF offered the advantage of the opportunity for efficiently processing small volumes of blood (2 mL), which is important in the care of pediatric patients, especially infants. The minimally invasive means of blood collection are of high value when treating patients whose MRD is typically tested using an invasive bone marrow biopsy. MF-MRD detection can be useful for stratification of patients into risk groups and monitoring of patient well-being after completion of treatment for early recognition of potential impending disease recurrence.
Ursolic Acid Regulates Immune Balance, Modulates Gut Microbial Metabolism, and Improves Liver Health in Mice
Authors: Zhao Man , Cui Yali , Wang Fengxia , Wu Fengyang , Li Chong , Liu Shudong , Chen Baojiang
Published: October 2024

Ursolic acid (UA) has demonstrated significant immunomodulatory and hepatoprotective effects; however, the underlying mechanisms remain unclear. This study aims to analyze the impact of UA on the gut microbiome, metabolome, and liver transcriptome, investigate UA’s role in maintaining gut immune homeostasis and liver health, and evaluate the potential contributions of gut microbes and their metabolites to these beneficial effects. Our findings indicate that UA enhances immune balance in the jejunum, fortifies intestinal barrier function, and promotes overall gut health. UA modulates the intestinal microbiota and its metabolic processes, notably increasing the abundance of beneficial bacteria such as Odoribacter and Parabacteroides, along with their metabolites, including ornithine and lactucin. Additionally, UA inhibits the expression of interleukin-1 receptor 1 (IL1R1) and calcium (Ca2+) voltage-gated channel auxiliary subunit beta 2 (CACNB2) while enhancing the synthesis pathways of retinol and ascorbic acid, thereby exerting a protective influence on liver function. In summary, UA enhances intestinal immune homeostasis and promotes liver health, with these advantageous effects potentially mediated by beneficial bacteria (Odoribacter and Parabacteroides) and their metabolites (ornithine and lactucin).
Self-Locking in Collapsed Carbon Nanotube Stacks via Molecular Dynamics
Authors: Pedrielli Andrea , Taioli Simone , Pugno Nicola Maria
Published: October 2024

Self-locking structures are often studied in macroscopic energy absorbers, but the concept of self-locking can also be effectively applied at the nanoscale. In particular, we can engineer self-locking mechanisms at the molecular level through careful shape selection or chemical functionalisation. The present work focuses on the use of collapsed carbon nanotubes (CNTs) as self-locking elements. We start by inserting a thin CNT into each of the two lobes of a collapsed larger CNT. We aim to create a system that utilises the unique properties of CNTs to achieve stable configurations and enhanced energy absorption capabilities at the nanoscale. We used molecular dynamics simulations to investigate the mechanical properties of periodic systems realised with such units. This approach extends the application of self-locking mechanisms and opens up new possibilities for the development of advanced materials and devices.
Comparison of Mitochondrial Genome Expression Differences among Four Skink Species Distributed at Different Latitudes under Low-Temperature Stress
Authors: Zhan Lemei , He Jingyi , Ding Lingyi , Storey Kenneth B. , Zhang Jiayong , Yu Danna
Published: October 2024

Continual climate change strongly influences temperature conditions worldwide, making ectothermic animals as suitable species for studying the potential impact of climate change on global biodiversity. However, the study of how lizards distributed at different latitudes respond to climate change at the transcriptome level is still insufficient. According to the Climatic Variability Hypothesis (CVH), the range of climate fluctuations experienced by terrestrial animals throughout the year increases with latitude, so individuals at higher latitudes should exhibit greater thermal plasticity to cope with fluctuating environments. Mitochondria, as the energy center of vertebrate cells, may indicate species’ plasticity through the sensitivity of gene expression. In this study, we focused on the changes in transcript levels of liver mitochondrial protein-coding genes (PCGs) in skinks from the genus Plestiodon (P. capito and P. elegans) and the genus Scincella (S. modesta and S. reevesii) under low-temperature conditions of 8 °C, compared to the control group at 25 °C. Species within the same genus of skinks exhibit different latitudinal distribution patterns. We found that the two Plestiodon species, P. elegans and P. capito, employ a metabolic depression strategy (decreased transcript levels) to cope with low temperatures. In contrast, the two Scincella species show markedly different patterns: S. modesta exhibits significant increases in the transcript levels of six genes (metabolic compensation), while in S. reevesii, only two mitochondrial genes are downregulated (metabolic depression) compared to the control group. We also found that P. capito and S. modesta, which live at mid-to-high latitudes, exhibit stronger adaptive responses and plasticity at the mitochondrial gene level compared to P. elegans and S. reevesii, which live at lower latitudes. We suggest that this enhanced adaptability corresponds to more significant changes in a greater number of genes (plasticity genes).
Localization and Tumor Growth Inhibition of I-131-Labeled Monoclonal Antibody ERIC1 in a Subcutaneous Xenograft Model of Small Cell Lung Cancer in SCID Mice
Authors: Fischer Thomas , Dietrich Christopher , Dietlein Felix , Muñoz Vázquez Sergio , Zimmermanns Beate , Krapf Philipp , Sudbrock Ferdinand , Drzezga Alexander , Dietlein Markus , Schomäcker Klaus
Published: October 2024

This study evaluates the efficacy of [131I]I-ERIC1 in targeting and inhibiting the growth of SCLC tumors in mice, focusing on tumor accumulation and regression and potential side effects. NCAM-positive NCI-H69 SCLC cells were implanted in CB 17 SCID mice, and [131I]I-ERIC1 biokinetics were measured in organs and tissues at four post-injection time points (24, 72, 96, and 120 h). The experimental series compared tumor growth, survival, and changes in blood counts among three treatment groups (1, 2, or 3 MBq) and a control group, with treatments initiated either two or five days post implantation. [131I]I-ERIC1 was synthesized with >95% radiochemical purity and a specific activity of 15 TBq/mmol. Tumor activity peaked at 31.5 ± 6.6% ID/g after four days, demonstrating significant antitumor efficacy, which resulted in sustained remission and extended survival. Hematological toxicity was observed, with the optimal dose identified as 2 MBq per animal administered two days post implantation. [131I]I-ERIC1 shows promise as a theranostic agent for personalized cancer treatment by effectively targeting SCLC tumors with manageable side effects. However, further studies are required to optimize dosing strategies and minimize toxicity.
Improvement of Quantification of Myocardial Synthetic ECV with Second-Generation Deep Learning Reconstruction
Authors: Morioka Tsubasa , Kato Shingo , Onoma Ayano , Izumi Toshiharu , Sakano Tomokazu , Ishikawa Eiji , Sawamura Shungo , Yasuda Naofumi , Nagase Hiroaki , Utsunomiya Daisuke
Published: October 2024

Background: The utility of synthetic ECV, which does not require hematocrit values, has been reported; however, high-quality CT images are essential for accurate quantification. Second-generation Deep Learning Reconstruction (DLR) enables low-noise and high-resolution cardiac CT images. The aim of this study is to compare the differences among four reconstruction methods (hybrid iterative reconstruction (HIR), model-based iterative reconstruction (MBIR), DLR, and second-generation DLR) in the quantification of synthetic ECV. Methods: We retrospectively analyzed 80 patients who underwent cardiac CT scans, including late contrast-enhanced CT (derivation cohort: n = 40, age 71 ± 12 years, 24 males; validation cohort: n = 40, age 67 ± 11 years, 25 males). In the derivation cohort, a linear regression analysis was performed between the hematocrit values from blood tests and the CT values of the right atrial blood pool on non-contrast CT. In the validation cohort, synthetic hematocrit values were calculated using the linear regression equation and the right atrial CT values from non-contrast CT. The correlation and mean difference between synthetic ECV and laboratory ECV calculated from actual blood tests were assessed. Results: Synthetic ECV and laboratory ECV showed a high correlation across all four reconstruction methods (R ≥ 0.95, p < 0.001). The bias and limit of agreement (LOA) in the Bland–Altman plot were lowest with the second-generation DLR (hybrid IR: bias = −0.21, LOA: 3.16; MBIR: bias = −0.79, LOA: 2.81; DLR: bias = −1.87, LOA: 2.90; second-generation DLR: bias = −0.20, LOA: 2.35). Conclusions: Synthetic ECV using second-generation DLR demonstrated the lowest bias and LOA compared to laboratory ECV among the four reconstruction methods, suggesting that second-generation DLR enables more accurate quantification.
The Prognostic Role of Advanced Lung Cancer Inflammation Index in Patients with Idiopathic Pulmonary Fibrosis
Authors: Bozkuş Fulsen , Keskin Olgun
Published: October 2024

Background: The advanced lung cancer inflammation index (ALI) is an innovative and thorough measure designed to assess both inflammation and nutritional status. It includes parameters such as albumin, body mass index (BMI), and the neutrophil-to-lymphocyte ratio (NLR). This research seeks to evaluate the prognosis of idiopathic pulmonary fibrosis (IPF) patients by integrating both inflammation and nutritional status, distinguishing it from conventional inflammation biomarkers. Methods: This study included 102 patients with IPF. Clinical data were extracted from the patients’ medical records. NLR and ALI scores were calculated based on data collected at the initiation of antifibrotic treatment using the following formulas: Neut/Lym for NLR and albumin × BMI/NLR for ALI. Results: ALI values were assessed across various IPF patient subgroups based on gender–age–physiology (GAP) stages (1, 2, and 3), forced vital capacity (FVC) (median split: <70% vs. ≥70%), diffusing capacity for carbon monoxide (DLCO) (<51% vs. ≥51%), 6-Minute Walk Test (6MWT) (<350 vs. ≥350), and the Charlson comorbidity index (CCI) (≤1 vs. >1). Significant differences in ALI were observed with respect to GAP stages, FVC, DLCO, and 6MWT categories (p = 0.000 for all), but not for CCI categories (p = 0.233). Receiver operating characteristic (ROC) curve analysis revealed that ALI had a sensitivity of 63.6% and a specificity of 98.9% at a threshold of 11.2 (AUC = 0.945, 95% CI 0.892–0.998, p < 0.000). Conclusions: Our findings indicate that ALI levels are significantly associated with disease severity and mortality in IPF patients.
Leukocyte Indices as Markers of Inflammation and Predictors of Outcome in Heart Failure with Preserved Ejection Fraction
Authors: Poledniczek Michael , Kronberger Christina , List Luca , Gregshammer Bernhard , Willixhofer Robin , Ermolaev Nikita , Duca Franz , Binder Christina , Rettl René , Badr Eslam Roza , Camuz Ligios Luciana , Nitsche Christian , Hengstenberg Christian , Kastner Johannes , Bergler-Klein Jutta , Kammerlander Andreas Anselm
Published: October 2024

Background: The pathophysiology of heart failure (HF) with preserved ejection fraction (HFpEF) is suggested to be influenced by inflammation. Leukocyte indices, including the neutrophil–lymphocyte ratio (NLR), the monocyte–lymphocyte ratio (MLR), and the pan-immune inflammation value (PIV), can be utilized as biomarkers of systemic inflammation. Their prognostic utility is yet to be fully understood. Methods: Between December 2010 and May 2023, patients presenting to a tertiary referral center for HFpEF were included into a prospective registry. The association of the NLR, MLR, and PIV with the composite endpoint of all-cause mortality and HF-related hospitalization was tested utilizing Cox regression analysis. Results: In total, 479 patients (median 74.3, interquartile range (IQR): 69.22–78.3 years, 27.8% male) were included. Patients were observed for 43 (IQR: 11–70) months, during which a total of 267 (55.7%) patients met the primary endpoint. In a univariate Cox regression analysis, an above-the-median NLR implied a hazard ratio (HR) of 1.76 (95%-confidence interval (CI): 1.38–2.24, p < 0.001), an MLR of 1.46 (95%-CI: 1.14–1.86, p = 0.003), and a PIV of 1.67, 95%-CI: 1.30–2.13, p < 0.001) for the composite endpoint. After adjustment in a step-wise model, the NLR (HR: 1.81, 95%-CI: 1.22–2.69, p = 0.003), the MLR (HR: 1.57, 95%-CI: 1.06–2.34, p = 0.026), and the PIV (HR: 1.64, 95%-CI: 1.10–2.46, p = 0.015) remained significantly associated with the combined endpoint. Conclusions: The NLR, the MLR, and the PIV are simple biomarkers independently associated with outcomes in patients with HFpEF.
Infective Endocarditis by Listeria Species—A Systematic Review
Authors: Kypraiou Despoina , Konstantaraki Maria , Tsantes Andreas G. , Ioannou Petros
Published: October 2024

Infective endocarditis (IE) is a disease associated with significant morbidity and mortality. It is more commonly caused by Gram-positive cocci, but Gram-positive bacilli may seldom cause the disease. Listeria monocytogenes is an aerobic Gram-positive coccobacillus and a foodborne and opportunistic pathogen most commonly causing gastrointestinal infections, even though bacteremia, sepsis, meningitis, and fetal infections may also occur. Listeria IE has rarely been described, with most reports being case reports or case series. Thus, the characteristics of this disease remain largely unknown. This systematic review aimed to present all published Listeria IE studies and describe their characteristics. A search of PubMed, Scopus, and the Cochrane Library for studies providing information on epidemiology, clinical findings, treatment, and outcome of Listeria IE cases was performed. A total of 54 studies containing data from 62 patients were included. Among all patients, 64.5% were male; the median age was 69 years. Among all patients, 54.8% had a history of a prosthetic valve. The aortic valve was the most commonly affected, followed by the mitral. Fever, heart failure, and embolic phenomena were the most commonly encountered clinical findings. The only isolated species was L. monocytogenes. Antimicrobial resistance was relatively low for aminopenicillins and aminoglycosides, the most commonly used antimicrobials for treating L. monocytogenes IE. Surgery was performed in 27.4% of patients. Mortality was 37.1%. Patients who survived were more likely to have had a prosthetic valve, to have necessitated transesophageal echocardiography for the diagnosis, to have mitral valve IE, and to have had surgical management; however, no factor was identified in a multivariate logistic regression model as an independent factor for overall mortality.
Rationale and Protocol of the ETERNITY-ITA Study: Use of Etelcalcetide for Preserving Vitamin K-Dependent Protein Activity—An Italian Study
Authors: Fusaro Maria , Aghi Andrea , Marino Carmela , Mallamaci Francesca , Plebani Mario , Zaninotto Martina , Grano Maria , Colucci Silvia , Gallieni Maurizio , Nickolas Thomas L. , Giannini Sandro , Sella Stefania , Simioni Paolo , Bazzocchi Alberto , Guglielmi Giuseppe , Taddei Fulvia , Schileo Enrico , Versace Maria Carmela , Tripepi Giovanni
Published: October 2024

Background/Objectives: Chronic kidney disease and mineral bone disorders (CKD-MBD) are frequently associated with an increased risk of both vascular calcifications (VCs) and bone fractures (BFs). The complex pathogenesis of VCs and BFs involves various factors such as calcium overload, phosphate imbalance, and secondary hyperparathyroidism. Key players, such as the vitamin K-dependent proteins (VKDPs) matrix Gla protein (MGP) and bone Gla protein (BGP), have pivotal roles both for VCs and BFs. The VIKI study highlighted that hemodialysis patients treated with calcimimetics had higher levels of total BGP and MGP compared to those untreated, suggesting a potential protective effect of these drugs on BFs and VCs beyond the beneficial effect of reducing PTH levels. Methods: ETERNITY-ITA is a multi-center, comparative effectiveness, observational, longitudinal study that will enroll 160 hemodialysis patients (80 patients treated with Etelcalcetide and 80 age- and sex-matched patients treated with calcitriol or vitamin D analogs). Nephrologists will tailor the target dose of Etelcalcetide on an individual level to achieve the KDIGO PTH target. In the Etelcalcetide-treated group, the addition of calcitriol will be allowed when required by clinical practice (for correction of hypocalcemia). Conclusions: This study will evaluate the real-world effect of Etelcalcetide on VKDP levels, such as BGP and MGP, at 3, 9, and 18 months from baseline. The resulting preservation of vascular and bone health will be assessed for the first time by examining aortic and iliac artery calcifications and vertebral fractures, respectively.