bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated multi-omics analysis describes immune profiles in ischemic heart failure and identifies PTN as a novel biomarker
Authors: Xiong Ting , Li Quhuan , Wang Yifan , Kong Ying , Li Hailin , Liu Jie , Wu Yueheng , Zhang Fengxia
Published: December 2024

Introduction

Heart failure is a leading global cause of mortality, with ischemic heart failure (IHF) being a major contributor. IHF is primarily driven by coronary artery disease, and its underlying mechanisms are not fully understood, particularly the role of immune responses and inflammation in cardiac muscle remodeling. This study aims to elucidate the immune landscape of heart failure using multi-omics data to identify biomarkers for preventing cardiac fibrosis and disease progression.

Methods

We utilized multi-omics data to elucidate the intricate immune landscape of heart failure at various regulatory levels. Given the substantial size of our transcriptomic dataset, we used diverse machine learning techniques to identify key mRNAs. For smaller datasets such as our proteomic dataset, we applied multilevel data cleansing and enhancement using principles from network biology. This comprehensive analysis led to the development of a scalable, integrated -omics analysis pipeline.

Results

Pleiotrophin (PTN) had shown significant upregulation in multiple datasets and the activation of various molecules associated with dysplastic cardiac remodeling. By synthesizing these data with experimental validations, PTN was identified as a potential biomarker.

Discussion

The present study not only provides a comprehensive perspective on immune dynamics in IHF but also offers valuable insights for the identification of biomarkers, discovery of therapeutic targets, and development of drugs.
Insulin like growth factor 2 mRNA binding protein 2 regulates vascular development in cerebral arteriovenous malformations
Authors: Wang Lin-jian , Wu Yangyang , Xie Sha , Lian Hongkai
Published: December 2024

Background

Cerebral arteriovenous malformations (AVMs) are intricate vascular anomalies that disrupt normal cerebral blood flow, potentially leading to severe neurological complications. Although the pathology of AVMs is not fully understood, epigenetic mechanisms have been implicated in their formation.

Methods

Transcriptional differences between cerebral AVMs and normal tissues were analyzed using RNA sequencing (RNA-seq), identifying IGF2BP2 as a key differentially expressed gene. Comprehensive bioinformatics analysis, integrating multi-omics data such as RNA-seq and methylated RNA immunoprecipitation sequencing (MeRIP-seq), was employed to identify the downstream target gene of IGF2BP2. The roles of specific genes in vascular development were assessed using endothelial cell cultures and zebrafish models.

Results

Our analysis of RNA-seq data from cerebral AVMs and normal tissues identified IGF2BP2, a key N6-methyladenosine (m6A) reader, as significantly downregulated in cerebral AVMs. Functional studies showed that IGF2BP2 knockdown resulted in abnormal angiogenesis in endothelial cells and disrupted vascular development in zebrafish models. Mechanistically, IGF2BP2 regulates LGALS8 expression by modulating mRNA stability through m6A modification, and LGALS8 deficiency severely impairs angiogenesis in vitro and leads to cerebrovascular dysplasia in vivo.

Conclusion

Our findings suggest that IGF2BP2, via m6A-dependent regulation of LGALS8, is crucial for vascular development and presents potential targets for therapeutic intervention in cerebral AVMs.
Association between lifelines diet score with odds of nonalcoholic fatty liver disease and some novel anthropometric indices among adults: a case–control study
Authors: Asiaei Sahar , Sharifani Mohammad Sharif , Ghobadian Bijan , Baghdadi Ghazal , Biglari Fereshteh , Rahimlou Mehran
Published: December 2024

Background

Nonalcoholic Fatty Liver Disease (NAFLD) is a prevalent condition strongly associated with poor dietary habits and obesity. The Lifelines Diet Score (LLDS), a measure of adherence to a health-promoting diet, may reduce the risk of NAFLD. This study investigates the association between LLDS and NAFLD risk, as well as its relationship with novel anthropometric indices in adults.

Methods

This case–control study included 180 NAFLD patients and 250 controls aged 20–65 years from Valiasr Hospital, Zanjan, Iran. Dietary intake was assessed using a validated 147-item food frequency questionnaire, and LLDS was calculated by scoring food groups according to dietary guidelines. Anthropometric measurements included Body Mass Index (BMI), Waist Circumference (WC), A Body Shape Index (ABSI), Body Roundness Index (BRI), and Visceral Adiposity Index (VAI). Logistic regression models estimated the odds ratios (ORs) for NAFLD across LLDS quartiles.

Results

Participants in the highest LLDS quartile had significantly reduced odds of NAFLD compared to those in the lowest quartile (OR = 0.49; 95% CI: 0.30–0.65; p < 0.001). Gender-specific analysis revealed that LLDS had a stronger inverse association with NAFLD in females (OR = 0.45; 95% CI: 0.29–0.64) than in males (OR = 0.63; 95% CI: 0.40–0.79). LLDS was inversely associated with VAI (β = −1.14; 95% CI: −2.89, −0.3; p = 0.036), but no significant associations were observed with ABSI or BRI.

Conclusion

Higher LLDS scores are associated with a lower risk of NAFLD and reduced visceral adiposity, particularly in females. These findings highlight the importance of improving dietary quality as a preventive strategy for NAFLD.
Prognostic significance and therapeutic potential of guanosine triphosphate cyclohydrolase 1 in esophageal squamous cell carcinoma: clinical implications of ferroptosis and lipid peroxidation regulation
Authors: Sakano Masayoshi , Tomita Yoshinobu , Kanazawa Takumi , Ishibashi Sachiko , Ikeda Masumi , Oshita Haruna , Hananoi Yuri , Kato Yuki , Yamamoto Kurara , Furukawa Asuka , Kinoshita Mayumi , Haruki Shigeo , Tokunaga Masanori , Kinugasa Yusuke , Kurata Morito , Kitagawa Masanobu , Ohashi Kenichi , Yamamoto Kouhei
Published: December 2024

Background

Esophageal cancer, particularly esophageal squamous cell carcinoma (ESCC), is a leading cause of cancer-related death and has a poor prognosis. Despite the advancements in multidisciplinary therapies, resistance to conventional treatments warrants the development of novel therapeutic strategies. Ferroptosis, a form of cell death dependent on intracellular iron, has emerged as a potential mechanism for targeting cancer cells resistant to apoptosis. Guanosine triphosphate cyclohydrolase 1 (GCH1) has been identified as a novel antagonist of ferroptosis; however, its role in ESCC remains unclear. This study aimed to investigate the correlation between the expression and accumulation of the lipid peroxidation markers and regulators, including GCH1, in patients with ESCC and examined their prognostic significance. Furthermore, we investigated the relationship between lipid peroxidation regulators and cell death using an in vitro system to establish the basis for new therapeutic strategies.

Methods

We retrospectively analyzed 312 patients with ESCC who underwent radical esophagectomy at the Tokyo Medical and Dental University. Immunohistochemistry was performed to evaluate the expression of lipid peroxidation markers (4-hydroxy-2-nonenal) and regulators (glutathione peroxidase 4 [GPX4], ferroptosis suppressor protein 1 [FSP1], and GCH1). The correlation between these markers, clinicopathological features, and overall survival was assessed. In vitro experiments were performed using KYSE-150 cells to investigate the effects of GCH1 knockdown and overexpression on cell proliferation, cisplatin-induced cell death, and ferroptosis.

Results

Low GCH1 expression was significantly associated with a poor prognosis in patients with ESCC. GCH1 expression correlated with lymph node metastases, vessel invasion, and the pathological tumor stage. In vitro, GCH1-knockdown cells exhibited increased proliferation and resistance to cisplatin-induced cell death, whereas GCH1 overexpression reduced cell proliferation. Simultaneous inhibition of GPX4 and FSP1 induced mild cell death; however, GCH1 knockdown dramatically enhanced ferroptosis, suggesting a synergistic effect.

Conclusion

GCH1 is a critical prognostic factor for ESCC and plays a significant role in the regulation of cell proliferation and ferroptosis. Targeting GCH1 in combination with GPX4 and FSP1 inhibitors may offer a novel therapeutic strategy for overcoming resistance in ESCC. Further studies are warranted to elucidate the involved molecular mechanisms and validate these findings in vivo.
Case report: Carcinosarcoma of uterus in nulliparous women
Authors: Sun Feiyue , Li Xuelei , Kang Luyao , Wang Yiran , Li Hongyu , Zhu Hai
Published: December 2024

Background

Uterine carcinosarcoma (UCS), or malignant mixed Müllerian tumor, is a cancer that include both carcinomatous and sarcomatous components, resembling endometrial carcinoma. A 55-year-old woman was admitted to the hospital with postmenopausal vaginal bleeding. Gross examination of the specimen revealed brittle tissue in the fundus and the left wall of the endometrium. Postoperative pathology revealed a mixture of well-differentiated endometrioid adenocarcinoma and osteosarcoma. The patient was never given birth, which may be relevant to the diagnosis. Literature review suggests that being nulliparous may be a significant risk factor for developing uterine carcinosarcoma.

Case description

In December 2023, a 55-year-old female patient was admitted to the hospital with postmenopausal vaginal bleeding. Hysteroscopic surgery was performed, and the postoperative pathology showed endometrial cancer accompanied by ossified tissue with necrosis. The immunohistochemical results indicated positive Estrogen receptors (ER), positive Progesterone receptors (PR), ki67 positivity at 70%, negative PTEN, mutated positive p53, focal positive Pax-8, positive SATB2, positive Cytokeratin 7 (CK7), positive EMA and positive Vimentin (Vim). The patient was diagnosed with Uterine carcinosarcoma. On December 18, 2023, the patient underwent partial vaginal resection, bilateral salpingo-oophorectomy, pelvic lymph node dissection, and a sub-extensive laparoscopic hysterectomy. Postoperatively, the patients received radiotherapy and four cycles of chemotherapy in the DC regimen. As of July 2024, laboratory and impact test results showed no tumor recurrence. The patient’s disease-free survival (DFS) was seven months.

Conclusion

The rate of childless in patients with uterine carcinosarcoma is at a high level.
Application of contrast-enhanced ultrasonography for gallbladder squamous cell carcinoma: a case report
Authors: Ruan Liqin , Wu Xiaoyong , Peng Guiping , Zhang Jing , Chen Weili
Published: December 2024

Preoperative diagnosis of Gallbladder squamous cell carcinoma (GBSCC) is difficult, and the contrast-enhanced ultrasound (CEUS) pattern has never been reported before. We present a case of GBSCC where CEUS revealed special findings that facilitated early diagnosis. CEUS demonstrated irregular peripheral ring-like enhancement during the arterial phase, with hypoenhancement in the late phases, and an irregular non-enhancing area persistently present in the center of the lesion.
Machine learning algorithms for the early detection of bloodstream infection in children with osteoarticular infections
Authors: Liu Yuwen , Wu Yuhan , Zhang Tao , Chen Jie , Hu Wei , Sun Guixin , Zheng Pengfei
Published: December 2024

Background

Bloodstream infection (BSI) poses a significant life-threatening risk in pediatric patients with osteoarticular infections. Timely identification of BSI is crucial for effective management and improved patient outcomes. This study aimed to develop a machine learning (ML) model for the early identification of BSI in children with osteoarticular infections.

Materials and methods

A retrospective analysis was conducted on pediatric patients diagnosed with osteoarticular infections admitted to three hospitals in China between January 2012 and January 2023. All patients underwent blood and puncture fluid bacterial cultures. Sixteen early available variables were selected, and eight different ML algorithms were applied to construct the model by training on these data. The accuracy and the area under the receiver operating characteristic (ROC) curve (AUC) were used to evaluate the performance of these models. The Shapley Additive Explanation (SHAP) values were utilized to explain the predictive value of each variable on the output of the model.

Results

The study comprised 181 patients in the BSI group and 420 in the non-BSI group. Random Forest exhibited the best performance, with an AUC of 0.947 ± 0.016. The model demonstrated an accuracy of 0.895 ± 0.023, a sensitivity of 0.847 ± 0.071, a specificity of 0.917 ± 0.007, a precision of 0.813 ± 0.023, and an F1 score of 0.828 ± 0.040. The four most significant variables in both the feature importance matrix plot of the Random Forest model and the SHAP summary plot were procalcitonin (PCT), neutrophil count (N), leukocyte count (WBC), and fever days.

Conclusions

The Random Forest model proved to be effective in early and timely identification of BSI in children with osteoarticular infections. Its application could aid in clinical decision-making and potentially mitigate the risk associated with delayed or inaccurate blood culture results.
A pilot study of the utility of a hospital-based school program for pediatric patients with cardiac diagnoses
Authors: Thibodaux Lia K. , Orr Ashley L. , Reisinger Debra L. , Fodstad Jill , Xu Guang , Wikel Kristin , Curtin Michelle
Published: December 2024

Introduction

Pediatric patients with complex cardiac diagnoses are at increased risk for physical, cognitive, and developmental complications. Formalized school support [i.e., individualized education programs (IEPs), Section 504 Accommodation Plans (Section 504 Plans)] that addresses the needs of these patients is necessary, and hospital-based school programs (HBSPs) have the potential to bolster the acquisition of academic support. In this pilot study, we look at the impact of one such HBSP.

Methods

Retrospective demographic and school support data for pediatric cardiac patients were analyzed.

Results

Our sample included 29 pediatric cardiac patients spanning two academic years. These patients had 100 HBSP encounters and 82 inpatient and 12 outpatient encounters, with 68.9% of patients having multiple encounters in a single year and 44.8% of patients being seen in both years. The HBSP made recommendations for patients to receive IEPs (N = 8) and Section 504 Plans (N = 13). The patients also submitted requests for medical homebound(N = 27), obtaining releases of information (N = 39), submitting medical reports (N = 10), and completing certificates of incapacity (N = 7). Statistical analyses revealed no significant relationships with patients entering or receiving a recommendation for an IEP or Section 504 Plan in any of their encounters with the HBSP on the basis of sex, race/ethnicity, school level, or rates of encounters in this sample.

Discussion

Similar to previous studies, these patients had high rates of IEPs/Section 504 Plans in place and continued to receive school recommendations through the HBSP. A high use of the HBSP was seen in the total number of encounters and communications (i.e., submitting to the school of record requests for classroom placement changes via medical homebound). Working with the HBSP provided access to information, formal support recommendations, and communication between medical and school settings in the form of changes in school status.
Reference ranges for complete blood count in children and adolescents with Down syndrome
Authors: Lattuada Martina , Capitoli Giulia , Casati Marco , Lazzerotti Alessandra , Maglia Oscar , Ferrari Giulia Maria , Fossati Chiara , Biondi Andrea , Cattoni Alessandro , Corti Paola
Published: December 2024

Introduction

Down syndrome (DS) is linked to unique hematopoietic characteristics that affect complete blood count (CBC) parameters. Accurate reference ranges are essential for proper CBC interpretation in this population.

Methods

This retrospective study analyzed 2,627 CBCs from 481 DS patients, aged 31 days to 18 years, at a tertiary care center in Italy. Patients with significant comorbidities were excluded to ensure a homogeneous group.

Results

CBC parameters were assessed to establish age- and sex-specific reference ranges. Centile charts were developed for each parameter, and an online tool was created to allow clinicians to compare individual CBC results with the new ranges. Comparisons with the general pediatric population revealed significant differences, particularly in hemoglobin, hematocrit, and mean corpuscular volume, which were higher in DS (p < 0.001). In contrast, a significant percentage of CBCs showed white blood cell counts below the 2.5th centile of healthy controls (p < 0.001), except for the 31 days–1 year age group. A similar trend was observed for lymphocytes (p < 0.001) in the 1-18 years group.

Discussion

These newly established DS-specific reference ranges provide clinicians with a crucial tool for evaluating CBC results, potentially reducing unnecessary tests and emphasizing the need for tailored clinical assessment in managing this unique population.
Ophthalmic corticosteroids-related adverse events: the FDA adverse event reporting system (FAERS) database pharmacovigilance study
Authors: Liu Chengzhi , Wang Xinyu , Cao Xusheng
Published: December 2024

Background

Corticosteroids are extensively used in ophthalmology, particularly for treating various inflammatory conditions. Despite their effectiveness, prolonged or high-dose corticosteroid use is associated with significant adverse drug reactions (ADRs), such as increased intraocular pressure, cataract formation, and secondary infections. However, there is currently no systematic study comparing the side effects of ophthalmic corticosteroids. This study aims to investigate the safety profiles of ophthalmic corticosteroids through pharmacovigilance analysis using the FDA Adverse Event Reporting System (FAERS) database.

Methods

We conducted a retrospective analysis of ADR reports related to commonly used ophthalmic corticosteroids from the FAERS database, covering the period from Q1 2004 to Q4 2023. Clinical features such as gender, age, administration route, and dosage form were also analyzed. Signal detection methods, including Reporting Odds Ratio (ROR), Proportional Reporting Ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), and the Multi-Item Gamma Poisson Shrinker (MGPS), were used to identify potential safety signals.

Results

A total of 9,854 ADRs related to ophthalmic corticosteroids were retrieved, with the most frequently reported drugs being Ozurdex (1,784 cases), Lotemax (3,239 cases), and Durezol (2,789 cases). Women accounted for a higher proportion of ADRs across most corticosteroids. ADR induction time analysis results showed that ADRs tend to occur in the early stages of drug use. The most common ophthalmic ADRs identified included eye inflammation, cataract, visual impairment, uveitis, eye pain, blurred vision, and retinal detachment. Additionally, Maxidex has been linked to endocrine disorders, while Ozurdex, Iluvien, and Triesence exhibited significant signals for product issues, likely related to their intraocular injection procedures. Notably, cataract was the most common PT among these drugs.

Conclusion

Our study reveals significant safety concerns related to using ophthalmic corticosteroids, particularly regarding adverse reactions that can impact visual function. These findings highlight the need for careful monitoring and individualized treatment plans to minimize the risk of ADRs in patients receiving corticosteroid therapy. Future studies combining FAERS data with large-scale clinical research are needed to explore these safety concerns further.
Hypoalbuminemia and cisplatin-induced acute kidney injury
Authors: Yin Wen-Jun , Huang Yu-Jie , Zhu Qing , Lin Xiao-Qing , Piao Hui-Ling , Yu Qian-Qian , Lai Chang-Hong , Zhou Guang-Liang , Zhou Ling-Yun , Liu Kun , Zuo Xiao-Cong , Zuo Shan-Ru
Published: December 2024

Background

Cisplatin binds to serum albumin in the body at a rate of 90%, and high levels of free cisplatin are a significant cause of its nephrotoxicity. Therefore, hypoalbuminemia theoretically poses a significant risk factor for cisplatin-induced acute kidney injury (CIA) and can be easily corrected. However, existing research results are inconsistent. Our aim is to confirm the association between hypoalbuminemia and CIA through a meta-analysis and a dual-center real-world data study.

Methods

First, we used a random-effects meta-analysis to summarize the odds ratio (OR) of the risk relationship between hypoalbuminemia and CIA. Then, we conducted a retrospective analysis of patients using cisplatin from Xiangya Third Hospital of Central South University (2014–2023) and Hunan Cancer Hospital (2019–2023) to analyze the relationship between hypoalbuminemia and CIA.

Results

The meta-analysis, which included six studies involving 4,359 cases, showed that hypoalbuminemia is associated with an increased risk of CIA (OR, 2.13; 95% CI, 1.37–3.32). A total of 5,452 and 25,214 patients from Xiangya Third Hospital and Hunan Cancer Hospital, respectively, were included. Both centers found a significant association between hypoalbuminemia and an increased risk of CIA (OR, 2.76; 95% CI, 1.94–3.93; OR, 2.88; 95% CI, 2.17–3.81), and the sensitivity analysis results were consistent.

Conclusion

Through meta-analysis and dual-center real-world data studies, we confirmed that hypoalbuminemia is an independent risk factor for CIA. Therefore, it is recommended that patients using cisplatin undergo serum albumin level testing and regular monitoring during treatment. Actively adjusting albumin levels may reduce the risk of CIA.
Influence of inhaled nitric oxide on bronchopulmonary dysplasia in preterm infants with PPHN or HRF at birth: a propensity score matched study
Authors: Huang Xue-rong , Wang Lian , Liang Guo-bao , Huang Sheng-qian , Feng Bao-ying , Zhu Lu , Fan Xu-fang , Yao Mu-lin , Zhang Jing , Wang Meng-jiao , Zheng Zhi , Zhu Yao , Duan Wen-li , Li Zhan-kui , Mao Jian , Ma Li , Xu Fa-lin , Wu Fan , Wei Qiu-fen , Liu Ling , Lin Xin-zhu
Published: December 2024

Background

Bronchopulmonary Dysplasia (BPD) is a chronic lung disease affecting preterm infants, with limited prevention and treatment options. Inhaled Nitric Oxide (iNO) is sometimes used to treat Persistent Pulmonary Hypertension of the Newborn (PPHN) and Hypoxemic Respiratory Failure (HRF), and its impact on BPD development remains debated.

Objective

To assess whether iNO-related factors are potential contributors to the development of BPD Grade Ⅱ-Ⅲ in very premature infants (VPI) diagnosed with PPHN or HRF at birth using Propensity Score Matching (PSM).

Methods

We conducted a retrospective cohort study of infants born at 22–32 weeks gestation with PPHN or HRF, treated with iNO for over 3 h. PSM matched groups by gestational age, birth weight, and gender, etc. Multivariate logistic regression evaluated the association between iNO treatment and BPD outcomes to identify influencing factors, while Restricted Cubic Spline (RCS) and mediation analysis examined iNO dose effects and potential mediators like mechanical ventilation time and oxygenation index (OI).

Results

A higher initial iNO dose was significantly associated with a reduced risk of BPD Grade Ⅱ-Ⅲ (adjusted OR = 0.68, 95% CI: 0.52–0.89, p < 0.01). Additionally, administration of iNO within the first 7 days of life was identified as an important influencing factor No significant mediation effects were observed for factors such as mechanical ventilation time and OI.

Conclusion

A higher initial iNO dose within the first 7 days was associated with a reduced risk of BPD Grade Ⅱ-Ⅲ in VPI with PPHN or HRF.
Milícias in Rio de Janeiro: deconstructing the myth of a violent everyday peace through a feminist perspective
Authors: Gouveia Junior Luis
Published: December 2024

Milícias have proliferated across Brazil, engaging in criminal activities while maintaining close connections to the Brazilian state and politicians. They seek political validation by presenting themselves as combating drug traffickers and reducing violence, positioning peace and security as fundamental to their political appeal. Therefore, examining the role of peace within these criminal organizations through the lens of peace and conflict studies provides valuable insights into how Brazilian militias work. Through an analysis of the rhetoric used by politicians, the media, and residents in areas under milícias’ control, it becomes apparent that these criminal groups assert their role in establishing a form of “everyday peace.” However, this form of peace is inherently violent, resulting in a paradoxical concept of ‘violent everyday peace.’ Consequently, the feminist framework’s emphasis on the public/private distinction and the continuum of violence becomes crucial in addressing this paradox. By applying this feminist concept, it becomes evident that milícias generate a limited sense of peace in the public sphere while perpetuating violence in the private domain. Private violence, however, is often overlooked by those unaffected by milícia control, allowing these groups to garner political support. These findings enrich the study of criminal organizations in Brazil and the concept of everyday peace, which has not been previously explored in the context of Brazilian milícias.
A cost-reducing reimbursement programme? Effects of value-based reimbursement on healthcare costs
Authors: Eriksson Thérèse , Tropp Hans , Wiréhn Ann-Britt , Levin Lars-Åke
Published: December 2024

Value-based reimbursement programmes have become increasingly common in attempts to bend the cost curve of healthcare without negative effects on quality. The aim of this study was to analyse the effect of introducing a value-based reimbursement programme on the cost to third-party payer. We performed a retrospective observational study with a before and after design based on the introduction of a value-based reimbursement programme in Sweden. We analysed patient level cost data from inpatient and outpatient care of patients undergoing lumbar spine surgery, 2006–2015. The average 1-year episode cost decreased 11 percent during the first 2 years with the value-based reimbursement. The number of patients increased 22 percent during the same period, causing the total cost to increase by 8 percent. The value to third-party payer increased after the introduction of the value-based reimbursement since more patients were treated and attained a positive outcome. The decreased episode cost may be a result of better coordinated post-discharge care. Another explanation could be that costs previously borne by the third-party payer are shifted onto the healthcare providers. Thus, it is crucial that providers find a sustainable way of delivering care in the long term to retain value. Interlinking patient records facilitates a holistic perspective among healthcare providers raising awareness of health care utilization through the whole care chain.
Prevalence of depression and anxiety among university students in Jeddah, Saudi Arabia: exploring sociodemographic and associated factors
Authors: Malebari Azizah M. , Alamoudi Saeed O. , AL-Alawi Talal I. , Alkhateeb Anas A. , Albuqayli Adnan S. , Alothmany Hamzah N.
Published: December 2024

Introduction

Previous studies have shown that mental health issues such as depression and anxiety are on the rise globally, particularly among university students. The aim of this study is to assess the prevalence of depressive and anxiety symptoms among university students, and the associated potential risk factors, in Jeddah, Saudi Arabia.

Methods

A cross-sectional questionnaire study was conducted in a sample of 728 students who anonymously completed three sets of questionnaires: a sociodemographic and lifestyle information questionnaire, the Patient Health Questionnaire-9 (PHQ-9) to screen for depressive symptoms and the Generalized Anxiety Disorder-7 (GAD-7) scale to screen for anxiety symptoms.

Results

In a survey of 728 students, 81.5% reported depression and 63.6% anxiety, with no difference between medical and non-medical majors. Regular exercise, a history of psychological disorders, a diagnosis of chronic illness, and the use of antidepressant medications were significant indicators of depression. Significant anxiety markers included female gender, a diagnosis of chronic illness, a history of psychological disorders, the use of antidepressant medications, and smoking. Surprisingly, we did not observe any significant differences between the prevalence of depression or anxiety symptoms and common risk predictors, such as poor academic performance, low family income, and year of study.

Conclusion

Depression and anxiety had a high prevalence among undergraduates, regardless of their field of study; therefore, we recommend the implementation and expansion of community-based mental health prevention programs and mentorship programs. Universities with counseling programs should identify and eliminate factors that contribute to depression and anxiety.
Catalyzing sustainable development: insights from the international workshop on STI policies and innovation systems in Central America
Authors: Huete-Pérez Jorge A. , Hernández-Mondragón Alma Cristal , Massey Douglas S. , Cumba García Luz M. , Amadei Bernard , De León Sautú Nadia , Acosta Maria L. , Asensio Omar , Boright John , Cosgrove Serena , Hernández Hernández Emilio , López-Selva María , Manfredi Juan L. , Mondragón Fanor , Natera José M. , Picardo Joao Oscar C. , Rivero Santos Angelo , Rocha Harold O.
Published: December 2024

This article examines the landscape of Science, Technology, and Innovation policies in Central America, focusing on Nicaragua, Guatemala, Honduras, and El Salvador. These nations face significant challenges in leveraging STI for sustainable development, including financial constraints and limited resources. Additionally, Central America struggles with systemic issues such as corruption, violence, and high levels of emigration, further complicating efforts to advance STI. A workshop organized by Georgetown University's Science Technology and International Affairs program brought together scholars to discuss STI policies, resulting in key recommendations. The article highlights critical challenges, including over-reliance on state funding, stagnant researcher numbers, and the pressing need for research diversification. It emphasizes the importance of youth engagement, leadership, and resilience in shaping effective STI policies. Recommendations include investing in science education, establishing governmental scientific advisory bodies, promoting research diversity, and addressing climate change through STI strategies. The findings provide valuable insights for scholars, policymakers, and international organizations working with less developed nations globally.
Case Report: A case study of positive doping control by animal-to-human drug transfer after an athlete administered medicine in spray format, containing clostebol acetate, to a pet dog
Authors: Pokrywka Andrzej , Sitkowski Dariusz , Surała Olga , Gheddar Laurie , Kintz Pascal
Published: December 2024

The presence of a doping substance in an athlete's biological sample may not be only related to intentional pharmacological support. The unintended use of a prohibited substance may be due various reasons. This paper describes the case of a Polish canoeist preparing for the 2024 Summer Olympics in Paris who presented a positive doping test result, as a consequence of administering medication to her injured dog. The athlete used a Trofodermin cutaneous spray (containing clostebol acetate) for pet treatment, which resulted in human transfer during close contact and subsequent detection by doping authorities. To bolster the athlete's defense, it was essential to substantiate the scenario of an unconscious violation of anti-doping rules with scientific evidence. Hence, the decision was made to analyze and compare samples of the athlete's hair and her dog's fur. This investigation confirmed that clostebol absorption occurred through the skin of the hands, transfer during sleeping with the dog on the same bedding and/or inhalation (during the application of the medication, which was dispensed to the animal's paws). This defense was accepted by the Court of Arbitration for the Sport Anti-Doping Division, which subsequently found that the athlete committed an anti-doping rule violation, but under circumstances that amounted to a “no fault” scenario.
Accuracy of self-reported foot strike pattern detection among endurance runners
Authors: Vincent Heather K. , Coffey Kyle , Villasuso Aiden , Vincent Kevin R. , Sharififar Sharareh , Pezzullo Lydia , Nixon Ryan M.
Published: December 2024

Introduction

Foot strike pattern is often associated with running related injury and the focus of training and rehabilitation for athletes. The ability to modify foot strike pattern depends on awareness of foot strike pattern before being able to attempt change the pattern. Accurate foot strike pattern detection may help prevent running related injury (RRI) and facilitate gait modifications and shoe transitions. The purposes of this study were to determine the accuracy of self-reported foot strike pattern among endurance runners, to identify what factors were predictive of accurate foot strike detection and recent RRI.

Methods

This was a retrospective, cross-sectional study which included endurance runners (N = 710; 51.5% female; 35.4 ± 15.5 years; 51.6% were training competitively at the time of testing) with different running injury histories. Runners self-reported foot strike pattern [rearfoot, non-rearfoot (mid or forefoot), or “don't know”] and information about shoewear specifics. All runners performed a single session of running at self-selected speed on an instrumented treadmill with 3D motion capture and high-speed filming that verified actual foot strike. Logistic regression was used to predict accuracy of foot strike detection and RRI.

Results

Overall accuracy of foot strike detection was low (42.7%; p < 0.01). Self-reported foot strike was 28.3% for rearfoot, 47.0% for nonrearfoot forefoot strike and 24.6% did not know. Biomechanical analyses actually showed that 34% of rearfoot strikers accurately detected rearfoot strike, while 69.5% of non-rearfoot strikers self-reported accurate non-rearfoot strike (p < 0.05). Runners who “did not know” their strike had the highest prevalence of RRI compared to runners who self-reported nonrearfoot or rearfoot strike (73% vs. 56% and 58%; p < .001). After accounting for several variables, shoe heel-to-toe drop was a consistent predictor of accurate strike detection [OR = 0.93 (0.88–0.99); p = 0.026] and RRI in last six months [OR = 1. 1 (1.01–1.17); p = 0.018]. RRI were also predicted by recent shoe change [OR = 2.8 (1.7–4.6); p < 0.001].

Discussion

Accurate detection of actual foot strike by endurance runners varies by the actual foot strike type determined during testing and is associated shoe characteristics. These findings demonstrate the importance of accurately identifying foot strike pattern and recommending footwear as a factor if planning to use retraining to alter foot strike pattern.
Meeting international self-report muscle strengthening guidelines is associated with better cardiovagal baroreflex sensitivity in adults
Authors: Waghorn Jocelyn , Schwartz Beverly D. , Shivgulam Madeline E. , Wu Yanlin , Kimmerly Derek S. , O’Brien Myles W.
Published: December 2024

Engaging in muscle strengthening activities (e.g., resistance training) at least twice/week is promoted by (Inter)national movement guidelines. Cardiovagal baroreflex sensitivity (cvBRS) reflects the ability to modulate R-R interval in response to changes in systolic blood pressure. Given the current conflicting literature, this study posed to explore the relationship between self-report muscle strengthening frequency and spontaneous cvBRS. 114 adults (62 females; age: 33 ± 19 years, 22% >55 years; body mass index: 24.2 ± 3.7 kg/m2) self-reported their weekly muscle strengthening activity frequencies via the Physical Activity and Sedentary Behaviour Questionnaire. Supine beat-by-beat R-R intervals [electrocardiography; 1.00 ± 0.18 s (0.90–1.50 s)] and systolic blood pressure [via finger photoplethysmography; 116 ± 11 mmHg (93–151 mmHg)] were recorded for 10.7 ± 2.0 min (5.3–14.5 min). Spontaneous cvBRS was assessed using the sequence technique. Data were analyzed using multiple regressions adjusted for age, sex, body mass index. Participants completed 2 ± 2 (0–7) days/week of muscle strengthening activities (56% met guidelines), and average overall cvBRS was 14.9 ± 9.1 (3.1–48.4) ms/mmHg. Higher reported frequencies were positively associated with overall cvBRS (Adjusted R2 = 0.40, p < 0.001; β = 2.24, p < 0.001). Meeting muscle strengthening activity guidelines was associated with improved overall cvBRS (Adjusted R2 = 0.29, p < 0.001; β = 7.68, p < 0.001). All results were unchanged if cvBRS for up-sequences or down-sequences only were used (all, p < 0.001). In conclusion, engaging in muscle strengthening exercises and particularly meeting existing guidelines were associated with better beat-by-beat vagally-mediated blood pressure regulation.
Unlocking the genetic secrets of Dorper sheep: insights into wool shedding and hair follicle development
Authors: Yuan Xiaochun , Meng Ke , Wang Yayan , Wang Yifan , Pan Cuili , Sun Haoran , Wang Jankui , Li Xinhai
Published: December 2024

Dorper sheep is popular among farming enterprises with strong adaptability, disease resistance, and roughage tolerance, and an unique characteristic of natural shedding of wool. In a large number of observations on experimental sheep farms, it was found that the wool of some sheep still had not shed after May, thus manual shearing was required. Therefore, understanding the molecular mechanisms of normal hair follicles (HFs) development is crucial to revealing the improvement of sheep wool-related traits and mammalian skin-related traits. In this study, transcriptome analysis was performed on skin tissues of adult Dorper ewes in the shedding (S) and non-shedding (N) groups in September 2019, January 2020, and March 2020, respectively. The results identified 3,278 differentially expressed transcripts (DETs) in the three comparison groups within the S group, 720 DETs in the three comparison groups within the N group, and 1,342 DETs in the three comparison groups between the S-vs-N groups. Time-series expression analysis revealed 2 unique expression patterns in HF development, namely, elevated expression in the anagen phase (A pattern) and the telogen phase (T pattern). DETs with stage-specific expression had a significant presence in processes related to the hair cycle and skin development, and several classic signaling pathways involved in sheep HF development, such as Rap1, estrogen, PI3K-Akt, and MAPK, were detected. Combined analysis of DETs, time-series expression data, and weighted gene co-expression network analysis identified core genes and their transcripts influencing HF development, such as DBI, FZD3, KRT17, ZDHHC21, TMEM79, and HOXC13. Additionally, alternative splicing analysis predicted that the isoforms XM_004004383.4 and XM_012125926.3 of ZDHHC21 might play a crucial role in sheep HF development. This study is a valuable resource for explaining the morphology of normal growth and development of sheep HFs and the genetic foundation of mammalian skin-related traits. It also offers potential insights into factors influencing human hair advancement.
Momentum-Based Adaptive Laws for Identification and Control
Authors: Somers Luke , Haddad Wassim M.
Published: December 2024

In this paper, we develop momentum-based adaptive update laws for parameter identification and control to improve parameter estimation error convergence and control system performance for uncertain dynamical systems. Specifically, we introduce three novel continuous-time, momentum-based adaptive estimation and control algorithms and evaluate their effectiveness via several numerical examples. Our proposed adaptive architectures show faster parameter convergence rates as compared to the classical gradient descent and model reference adaptive control methods.
Improved Field Obstacle Detection Algorithm Based on YOLOv8
Authors: Zhou Xinying , Chen Wenming , Wei Xinhua
Published: December 2024

To satisfy the obstacle avoidance requirements of unmanned agricultural machinery during autonomous operation and address the challenge of rapid obstacle detection in complex field environments, an improved field obstacle detection model based on YOLOv8 was proposed. This model enabled the fast detection and recognition of obstacles such as people, tractors, and electric power pylons in the field. This detection model was built upon the YOLOv8 architecture with three main improvements. First, to adapt to different tasks and complex environments in the field, improve the sensitivity of the detector to various target sizes and positions, and enhance detection accuracy, the CBAM (Convolutional Block Attention Module) was integrated into the backbone layer of the benchmark model. Secondly, a BiFPN (Bi-directional Feature Pyramid Network) architecture took the place of the original PANet to enhance the fusion of features across multiple scales, thereby increasing the model’s capacity to distinguish between the background and obstacles. Third, WIoU v3 (Wise Intersection over Union v3) optimized the target boundary loss function, assigning greater focus to medium-quality anchor boxes and enhancing the detector’s overall performance. A dataset comprising 5963 images of people, electric power pylons, telegraph poles, tractors, and harvesters in a farmland environment was constructed. The training set comprised 4771 images, while the validation and test sets each consisted of 596 images. The results from the experiments indicated that the enhanced model attained precision, recall, and average precision scores of 85.5%, 75.1%, and 82.5%, respectively, on the custom dataset. This reflected increases of 1.3, 1.2, and 1.9 percentage points when compared to the baseline YOLOv8 model. Furthermore, the model reached 52 detection frames per second, thereby significantly enhancing the detection performance for common obstacles in the field. The model enhanced by the previously mentioned techniques guarantees a high level of detection accuracy while meeting the criteria for real-time obstacle identification in unmanned agricultural equipment during fieldwork.
Correction: Kim et al. Spatial Correlations between Nitrogen Budgets and Surface Water and Groundwater Quality in Watersheds with Varied Land Covers. Agriculture 2024, 14, 429
Authors: Kim Deok-Woo , Chung Eu Gene , Na Eun Hye , Kim Youngseok
Published: December 2024

Missing Funding [...]
Effects of Biological Additives on the Fermentation Quality and Microbial Community of High-Moisture Oat Silage
Authors: Huang Yifei , Zhang Siling , Hu Maoling , Yang Shuyuan , Hui Ailing , Zhang Wencheng , Wu Zeyu
Published: December 2024

The primary objective of this study was to explore the effects of biological additives, including Streptococcus bovis (SB), Bacillus subtilis (BS), xylanase (XT), and their combined treatments, including SB + BS (SBBS), SB + XT (SBXT), and BS + XT (BSXT), on the chemical composition, fermentation characteristics, and microbial community of high-moisture oat silage. Compared with the CK group (control group without additives), SB and SBBS treatments increased the lactic acid content (p < 0.05) and reduced the contents of acetic acid, propionic acid, butyric acid, and ammonia nitrogen in silage (p < 0.05). XT, SBXT, and BSXT treatments decreased the neutral detergent fiber and acid detergent fiber contents (p < 0.05), increasing the water-soluble carbohydrate content (p < 0.05). The SB, SBBS, and SBXT treatments increased the abundance of Lactiplantibacillus (p < 0.05) and significantly decreased microbial richness with diversity (p < 0.05), improving the microbial community structure in silage. The addition of XT increased the relative abundance of Clostridium and Enterobacteriaceae, but its combination with SB and BS increased the abundance of Lactiplantibacillus and inhibited the development of undesirable bacteria. Moreover, different additives changed the metabolism of carbohydrates, amino acids, energy, cofactors and vitamins of bacterial communities during ensiling. In summary, the addition of SB and SBBS was more conducive to improving the fermentation characteristics of oat, while XT, SBXT, and BSXT performed better in degrading lignocellulose in plants.
Next-Generation Nitrate, Ammonium, Phosphate, and Potassium Ion Monitoring System in Closed Hydroponics: Review on State-of-the-Art Sensors and Their Applications
Authors: Hong Yeonggeeol , Lee Jooyoung , Park Sangbae , Kim Jangho , Jang Kyoung-Je
Published: December 2024

Closed hydroponics is an environmentally friendly and economical method for growing crops by circulating a nutrient solution while measuring and supplementing various ions contained in the solution. However, conventional monitoring systems in hydroponics do not measure individual ions in the nutrient solution; instead, they predict the total ion content from the pH and electrical conductivity (EC). This method cannot be used to supplement individual ions and adjusts the concentration of the circulating nutrient solution by diluting or supplying a premixed nutrient solution. A more advanced system should be able to identify the concentration of each ion in the nutrient solution and supplement any deficient ions, thus requiring individual ion monitoring systems. Therefore, we first investigated the nitrate, ammonium, phosphate, and potassium (NPK) ion concentration and pH range commonly used for nutrient solutions. Subsequently, we discuss the latest research trends in electrochemical and optical sensors for measuring NPK ions. We then compare the conventional monitoring system (pH and EC-based) and advanced monitoring systems (individual ion sensors) and discuss the respective research trends. In conclusion, we present the hurdles that researchers must overcome in developing agricultural ion sensors for advanced monitoring systems and propose the minimum specifications for agricultural NPK ion sensors.