bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of the Equity in Formation of Investment Portfolio of a Shipping Company
Authors: Bazaluk Oleg , Zhykharieva Vlada , Vlasenko Olga , Nitsenko Vitalii , Streimikiene Dalia , Balezentis Tomas
Published: January 2022

The development of shipping companies relies on multiple financing channels and requires decisions to be made regarding fleet management. Therefore, a firm’s performance should be modeled based on multiple decision variables. The purpose of this paper is to develop a quantitative approach for determining the investment portfolio of a shipping company taking into account the equity value. The proposed model rests on the mathematical programming. The objective is to maximize the cumulative free cash flow to equity, associated with the implementation of a long-term fleet replenishment program. The distinctive feature of the approach is that different cost of debt financing options and different levels of starting capital are considered. The enterprise can then be determined based on the optimal value of free cash flows to equity for each ratio of starting capital and the number of acquired vessels.
Homogeneous Banach Spaces as Banach Convolution Modules over M(G)
Authors: Feichtinger Hans Georg
Published: January 2022

This paper is supposed to form a keystone towards a new and alternative approach to Fourier analysis over LCA (locally compact Abelian) groups G. In an earlier paper the author has already shown that one can introduce convolution and the Fourier–Stieltjes transform on (M(G),∥·∥M), the space of bounded measures (viewed as a space of linear functionals) in an elementary fashion over Rd. Bounded uniform partitions of unity (BUPUs) are easily constructed in the Euclidean setting (by dilation). Moving on to general LCA groups, it becomes an interesting challenge to find ways to construct arbitrary fine BUPUs, ideally without the use of structure theory, the existence of a Haar measure and even Lebesgue integration. This article provides such a construction and demonstrates how it can be used in order to show that any so-called homogeneous Banach space(B,∥·∥B) on G, such as (Lp(G),∥·∥p), for 1≤p<∞, or the Fourier–Stieltjes algebra FM(G), and in particular any Segal algebra is a Banach convolution module over (M(G),∥·∥M) in a natural way. Via the Haar measure we can then identify L1(G),∥·∥1 with the closure (of the embedded version) of Cc(G), the space of continuous functions with compact support, in (M(G),∥·∥M), and show that these homogeneous Banach spaces are essentialL1(G)-modules. Thus, in particular, the approximate units act properly as one might expect and converge strongly to the identity operator. The approach is in the spirit of Hans Reiter, avoiding the use of structure theory for LCA groups and the usual techniques of vector-valued integration via duality. The ultimate (still distant) goal of this approach is to provide a new and elementary approach towards the (extended) Fourier transform in the setting of the so-called Banach–Gelfand triple(S0,L2,S0′)(G), based on the Segal algebra S0(G). This direction will be pursued in subsequent papers.
Stochastic Analysis of Nonlinear Cancer Disease Model through Virotherapy and Computational Methods
Authors: Raza Ali , Awrejcewicz Jan , Rafiq Muhammad , Ahmed Nauman , Mohsin Muhammad
Published: January 2022

Cancer is a common term for many diseases that can affect anybody. A worldwide leading cause of death is cancer, according to the World Health Organization (WHO) report. In 2020, ten million people died from cancer. This model identifies the interaction of cancer cells, viral therapy, and immune response. In this model, the cell population has four parts, namely uninfected cells (x), infected cells (y), virus-free cells (v), and immune cells (z). This study presents the analysis of the stochastic cancer virotherapy model in the cell population dynamics. The model results have restored the properties of the biological problem, such as dynamical consistency, positivity, and boundedness, which are the considerable requirements of the models in these fields. The existing computational methods, such as the Euler Maruyama, Stochastic Euler, and Stochastic Runge Kutta, fail to restore the abovementioned properties. The proposed stochastic nonstandard finite difference method is efficient, cost-effective, and accommodates all the desired feasible properties. The existing standard stochastic methods converge conditionally or diverge in the long run. The solution by the nonstandard finite difference method is stable and convergent over all time steps.
Recent Advances in Robust Design for Accelerated Failure Time Models with Type I Censoring
Authors: Rivas-López María J. , Martín-Martín Raúl , García-Camacha Gutiérrez Irene
Published: January 2022

Many fields including clinical and manufacturing areas usually perform life-testing experiments and accelerated failure time models (AFT) play an essential role in these investigations. In these models the covariate causes an accelerant effect on the course of the event through the term named acceleration factor (AF). Despite the influence of this factor on the model, recent studies state that the form of AF is weakly or partially known in most real applications. In these cases, the classical optimal design theory may produce low efficient designs since they are highly model dependent. This work explores planning and techniques that can provide the best robust designs for AFT models with type I censoring when the form of the AF is misspecified, which is an issue little explored in the literature. Main idea is focused on considering the AF to vary over a neighbourhood of perturbation functions and assuming the mean square error matrix as the basis for measuring the design quality. A key result of this research was obtaining the asymptotic MSE matrix for type I censoring under the assumption of known variance regardless the selected failure time distribution. In order to illustrate the applicability of previous result to a study case, analytical characterizations and numerical approaches were developed to construct optimal robust designs under different contaminating scenarios for a failure time following a log-logistic distribution.
Convergence Investigation of XFEM Enrichment Schemes for Modeling Cohesive Cracks
Authors: Liu Guangzhong , Guo Jiamin , Bao Yan
Published: January 2022

When simulating cohesive cracks in the XFEM framework, specific enrichment schemes are designed for the non-singular near-tip field and an iteration procedure is used to solve the nonlinearity problem. This paper focuses on convergence and accuracy analysis of XFEM enrichment schemes for cohesive cracks. Four different kinds of enrichment schemes were manufactured based on the development of XFEM. A double-cantilever beam specimen under an opening load was simulated by Matlab programming, assuming both linear and exponential constitutive models. The displacement and load factors were solved simultaneously by the Newton–Raphson iterative procedure. Finally, based on a linear or an exponential constitutive law, the influences of variations in these enrichment schemes, including (i) specialized tip branch functions and (ii) corrected approximations for blending elements, were determined and some conclusions were drawn.
Expression of Selected Genes and Circulating microRNAs in Patients with Celiac Disease
Authors: Domsa Elena Maria , Berindan-Neagoe Ioana , Budisan Livia , Braicu Cornelia , Para Ioana , Tantau Alina Ioana , Orasan Olga Hilda , Ciobanu Lidia , Pop Teodora Atena , Filip Gabriela Adriana , Leach Nicoleta , Negrean Vasile , Matei Daniela , Andreica Vasile
Published: January 2022

Background and Objectives: Celiac disease (CD) is an immune-mediated enteropathy with characteristic intestinal alterations. CD occurs as a chronic inflammation secondary to gluten sensitivity in genetically susceptible individuals. Until now, the exact cause of the disease has not been established, which is why new studies have appeared that address the involvement of various genes and microRNAs (miRNAs) in the pathogenesis. The aim of the study is to describe the expression of selected genes (Wnt family member 3, WNT3; Wnt family member 11, WNT11; tumor necrosis factor alpha, TNFα; mitogen-activated protein kinase 1, MAPK1; AKT serine/threonine kinase 3, AKT3; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha, PIK3CA; and cyclin D1, CCND1) and miRNAs (miR-192-5p, miR-194-5p, miR-449a and miR-638) in adult patients with CD. Materials and Methods: In total, 15 patients with CD at diagnosis (newly diagnosed), 33 patients on a gluten-free diet (GFD) for at least 1 year and 10 controls (control) were prospectively included. Blood samples were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: The results show that TNFα, MAPK1 and CCND1 were significantly overexpressed (p = 0.0249, p = 0.0019 and p = 0.0275, respectively) when comparing the newly diagnosed group to the controls. The other genes studied in CD patients were mostly with high values compared to controls, without reaching statistical significance. Among the miRNAs, the closest to a statistically significant value was miR-194-5p when the newly diagnosed group versus control (p = 0.0510) and GFD group versus control (p = 0.0671) were compared. The DIANA and miRNet databases identified significant functional activity for miR-449a and miR-192-5p and an interconnection of miR-194-5p and miR-449a with CCND1. Conclusions: In conclusion, genes and circulating miRNAs require further studies as they could represent important biomarkers in clinical practice.
Left Atrial Fibroelastoma as a Cause of Stroke: A Case Report
Authors: Lin Jing-Chun , Tsai Szu-Yu , Kuan Ta-Shen , Lin Wei-Pin
Published: January 2022

Cardiac papillary fibroelastoma is a benign and rare primary tumor of the heart that is most frequently located in the aortic or the mitral valves. Papillary fibroelastoma arising from the left atrium is exceedingly rare, comprising less than 7% of all cardiac papillary fibroelastomas. Tumors in this location could be a source of cardioembolic stroke, often in the anterior circulation of the cerebrum. A 66-year-old female presenting with right hemiparesis, central facial palsy, homonymous hemianopia, and dysarthria received intravenous thrombolysis for stroke without apparent improvement. Magnetic resonance imaging of the brain revealed ischemic infarction in the territories of the left middle and posterior cerebral arteries. A tumor with a maximal diameter of 2.3 cm was disclosed during workup for possible cardioembolic stroke with transthoracic echocardiography and computed tomography of the heart. The clinical course was complicated by stroke-in-evolution and hemorrhagic transformation. The patient underwent left atrial tumor excision and left atrium appendage closure. In-patient stroke rehabilitation programs were also initiated after the conditions stabilized. No clinically significant complications developed after the operation. Neurological functions improved and the patient was able to perform most basic daily living activities with some assistance. An exhaustive search for the cause of cardioembolic stroke is paramount, as management strategies may differ from patients with thrombotic stroke.
Vesiculobullous Diseases
Authors: Huang Simo , Hsu Sylvia , Motaparthi Kiran
Published: January 2022

A diverse range of inflammatory dermatoses are characterized by vesicles or bullae [...]
Polybenzimidazole Membrane Crosslinked with Epoxy-Containing Inorganic Networks for Organic Solvent Nanofiltration and Aqueous Nanofiltration under Extreme Basic Conditions
Authors: Lee Jaewon , Yang Hyeonmin , Bae Tae-Hyun
Published: January 2022

In this study, a novel polybenzimidazole (PBI)-based organic solvent nanofiltration (OSN) membrane possessing excellent stability under high pH condition was developed. To improve the chemical stability, the pristine PBI membrane was crosslinked with a silane precursor containing an epoxy end group. In detail, hydrolysis and condensation reaction of methoxysilane in the 3-glycidyloxypropyl trimethoxysilane (GPTMS) yields organic–inorganic networks within the PBI membrane structure. At the same time, the epoxy end groups on the organosiloxane network (Si–O–Si) reacted with amine groups of PBI to complete the crosslinking. The resulting crosslinked PBI membrane exhibited a good stability upon exposure to organic solvents and was not decomposed even in basic solution (pH 13). Our membrane showed an ethanol permeance of 27.74 LMHbar−1 together with a high eosin Y rejection of >90% under 10 bar operation pressure at room temperature. Furthermore, our PBI membrane was found to be operational even under an extremely basic condition, although the effective pore size was slightly enlarged due to the pore swelling effect. The results suggest that our membrane is a promising candidate for OSN application under basic conditions.
Preparation and Characterization of the Forward Osmosis Membrane Modified by MXene Nano-Sheets
Authors: Nie Yuqi , Xie Chaoxin , Wang Yi
Published: January 2022

The Forward Osmosis (FO) membrane was the core of FO technology. Obtaining a high water flux while maintaining a low reverse solute flux has historically been considered the gold standard for a perfect FO membrane. In a thin-film composite FO membrane, the performance of the membrane was determined not only by the material and structure of the porous support layer but also by the structural and chemical properties of the active selective layer. Researchers have selected numerous sorts of materials for the FO membranes in recent years and have produced exceptional achievements. Herein, the performance of the modified FO membrane constructed by introducing new two-dimensional nanomaterial MXene nano-sheets to the interfacial polymerization process was investigated, and the performance of these modified membranes was investigated using a variety of characterization and testing methods. The results revealed that the MXene nano-sheets played an important role in improving the performance of the FO membrane. Because of the hydrophilic features of the MXene nano-sheets, the membrane structure may be tuned within a specific concentration range, and the performance of the modified FO membrane has been significantly enhanced accordingly. The optimal membrane water flux was boosted by around 80%, while its reverse solute flux was kept to a minimum of the resultant membranes. It showed that the addition of MXene nanosheets to the active selective layer could improve the performance of the FO membrane, and this method showed promising application prospects.
Investigation of NH3 Desorption Kinetics on the LTA and SOD Zeolite Membranes
Authors: Gordina Natalya E. , Borisova Tatyana N. , Klyagina Ksenya S. , Astrakhantseva Irina A. , Ilyin Alexander A. , Rumyantsev Ruslan N.
Published: January 2022

The acidity characteristics of zeolite are highly significant, and understanding the acidic properties is essential for developing new types of zeolite catalysts. Zeolite membranes were synthesized using metakaolin, sodium hydroxide, and alumina with a molar ratio of 6Al2Si2O7:12NaOH:2Al2O3 as the starting ingredients. X-ray diffraction, scanning electron microscopy, and infrared spectroscopy were used for this study. N2 adsorption measurements determined the surface areas of the SOD zeolite membrane (115 m2/g) and the LTA membrane (150 m2/g). The units of absorbed water vapor were 40 and 60 wt% for the SOD membrane and the LTA membrane, respectively. The strength and number of acid sites of the synthesized LTA and SOD zeolite membranes were determined by temperature-programmed desorption of ammonia. As a result, the value of the total acidity of the LTA zeolite membrane is in the range of 0.08 × 1019 units/m2 while that of the sodalite membrane is an order of magnitude lower and is 0.006 × 1019 units/m2. The apparent activation energy values for desorption of ammonia from LTA and SOD zeolite membranes were calculated using data on the kinetics of desorption of ammonia at different heating rates. It was found that at temperatures below 250 °C, the degree of conversion of the activation energy values is no more than 35 kJ/mol, which corresponds to the desorption of physically bound ammonia. An increase in the activation values up to 70 kJ/mol (for SOD) and up to 80 kJ/mol (for LTA) is associated with the desorption of chemically bound ammonia from the samples.
The Microstructure and Mechanical Properties of 5083, 6005A and 7N01 Aluminum Alloy Gas Metal Arc-Welded Joints for High-Speed Train: A Comparative Study
Authors: Wu Laijun , Yang Biao , Han Xiaohui , Ma Guolong , Xu Bingxiao , Liu Yuhang , Song Xiaoguo , Tan Caiwang
Published: January 2022

This study aimed to conduct a comparative study on the microstructure and mechanical performance of 5083, 6005A and 7N01 Al joints used in China Railway High-speed (CRH) trains. We connected 10 mm-thick plates by three-layer and three-pass gas metal arc welding (GMAW). The results indicated that 6005A and 7N01 Al joints were more sensitive to grain boundary liquation in the partially melted zone (PMZ) than 5083 Al joins. Besides, recrystallization was obtained in heat-affected zones (HAZ). The 5083 Al joints experienced the most severe recrystallization and the grain size changed from 6.32 (BM) to 32.44 (HAZ) μm duo to intracrystalline strain induced by cold-rolled processes. The 7N01 Al alloys experienced the lowest extent of recrystallization and the grain size increased from 5.32 (BM) to 22.31 (HAZ) μm. Furthermore, significant precipitate evolution in the HAZ was observed. Original thin β” precipitates dissolved in HAZ of 6005A Al joints and transformed to the softer β phase. However, less precipitation transition was examined in 5083 and 7N01 Al joints. The precipitates’ evolution produced a softening region in HAZ of 6005A joints where the hardness was only 55 HV. The microhardness profile of the other two Al joints was less affected. The tensile strength of 5083, 6005A, and 7N01 Al alloy joints reached 323, 206 and 361 MPa, respectively. The 5083 Al and 6005A Al joints failed at HAZ near the fusion line while 7N01 Al joints failed at the fusion zone owing to the high strength of the base metal. The liquation, coarse grains by recrystallization, and precipitate evolution all decreased local strength, resulting in the fracture at HAZ.
Damage Analysis of Third-Generation Advanced High-Strength Steel Based on the Gurson–Tvergaard–Needleman (GTN) Model
Authors: Santos Rafael O. , Moreira Luciano P. , Butuc Marilena C. , Vincze Gabriela , Pereira António B.
Published: January 2022

The third generation of advanced high-strength steels (AHSS) brought attention to the steel and automotive industries due to its good compromise between formability and production costs. This work evaluated a third-generation AHSS (USS CR980XG3TM) through microstructural and X-ray diffraction (XRD) analyses, uniaxial tensile and plane-strain tension testing, and numerical simulations. The damage behavior of this steel is described with the Gurson–Tvergaard–Needleman (GTN) model using an identification procedure based on the uniaxial tensile and initial microvoids data. The microstructure of the CR980XG3TM steel is composed of ferrite, martensite–austenite islands, and retained austenite with a volume fraction of 12.2%. The global formability of the CR980XG3TM steel, namely the product of the uniaxial tensile strength and total elongation values, is 24.3 GPa%. The Lankford coefficient shows a weak initial plastic anisotropy of the CR980XG3TM steel with the in-plane anisotropy close to zero (−0.079) and the normal anisotropy close to unity (0.917). The identified GTN parameters for the CR980XG3TM steel provided a good forecast for the limit strains defined according to ISO 12004-2 standard from the uniaxial tensile and plane-strain tension data.
Al Alloys and Casting Processes for Induction Motor Applications in Battery-Powered Electric Vehicles: A Review
Authors: Li Yuxian , Hu Anita , Fu Yintian , Liu Sufeng , Shen Wutian , Hu Henry , Nie Xueyuan
Published: January 2022

With the rapid expansion of battery-powered electric vehicles (BEVs) in the automotive industry, research interest in lightweight Al alloys as well as their casting processes and applications has increased considerably. The substitution of castable aluminum alloys with superior strengths and electrical conductivity for copper reduces the weight and size of electric induction motors, and improves the energy efficiency and driving range of the BEVs. The present article was intended to give a general introduction into the common cast Al aluminum alloys and their relevant processes, as well as to motivate the development of high strength and conductive Al alloys for the practical realization of Al applications in the motors of the BEVs. A number of cast alloy systems containing Cu, Si, Ni, Mg, Fe, and Ti were evaluated, in comparison to nanostructured wrought Al alloys. The conventional casting processes suitable for Al alloys, high pressure die casting, squeeze casting, and sand casting were described. Strengthening mechanisms including solid solution strengthening, precipitation strengthening, dislocation accumulation strengthening, and grain boundary strengthening were presented. The phenomenon of electrical conduction for Al alloys was outlined. The mechanical properties and electrical properties of the recently developed Al alloys for casting and deformation processes were comprehensively listed and critically reviewed in association with microstructural characteristics.
Discrete Event Simulation for Machine-Learning Enabled Mine Production Control with Application to Gold Processing
Authors: Peña-Graf Felipe , Órdenes Javier , Wilson Ryan , Navarra Alessandro
Published: January 2022

Interdisciplinary barriers separating data scientists and geometallurgists have complicated systematic attempts to incorporate machine-learning into mine production management; however, experiences in excavating a vein-hosted gold deposit within the Alhué region of Chile have led to methodological advances, which is the subject of the current paper. These deposits are subject to several challenges, from increasing orebody complexity and decreasing gold grades to the significant geological uncertainty that is intrinsic to these systems. These challenges then translate to mineral processing, which is already dealing with increased environmental and technological constraints. Geological uncertainty causes stockout risks that can be mitigated by the approach that is developed within this paper, which features alternate operational modes and related control strategies. A digital twin framework based on discrete event simulation (DES) and a customized machine-learning (ML) model is proposed to incorporate geological variation into decision-making processes, including the setting of trigger point that induces mode changes. Sample calculations that were based on a simulated processing plant that was subject to mineralogical feed changes demonstrated that the framework is a valuable tool to evaluate and mitigate the potential risks to gold mineral processing performance.
Postmortem Metabolomics Reveal Acylcarnitines as Potential Biomarkers for Fatal Oxycodone-Related Intoxication
Authors: Elmsjö Albert , Söderberg Carl , Jakobsson Gerd , Green Henrik , Kronstrand Robert
Published: January 2022

Postmortem metabolomics has recently been suggested as a potential tool for discovering new biological markers able to assist in death investigations. Interpretation of oxycodone concentrations in postmortem cases is complicated, as oxycodone tolerance leads to overlapping concentrations for oxycodone intoxications versus non-intoxications. The primary aim of this study was to use postmortem metabolomics to identify potential endogenous biomarkers that discriminate between oxycodone-related intoxications and non-intoxications. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry data from 934 postmortem femoral blood samples, including oxycodone intoxications and controls positive and negative for oxycodone, were used in this study. Data were processed and evaluated with XCMS and SIMCA. A clear trend in group separation was observed between intoxications and controls, with a model sensitivity and specificity of 80% and 76%. Approximately halved levels of short-, medium-, and long-chain acylcarnitines were observed for oxycodone intoxications in comparison with controls (p < 0.001). These biochemical changes seem to relate to the toxicological effects of oxycodone and potentially acylcarnitines constituting a biologically relevant biomarker for opioid poisonings. More studies are needed in order to elucidate the potential of acylcarnitines as biomarker for oxycodone toxicity and their relation to CNS-depressant effects.
A Comparison of Mother’s Milk and the Neonatal Urine Metabolome: A Unique Fingerprinting for Different Nutritional Phenotypes
Authors: Dessì Angelica , Marzullo Alessandra , Corbu Sara , Bosco Alice , Cesare Marincola Flaminia , Pattumelli Maria Grazia , Mussap Michele , Pintus Roberta , Fanos Vassilios , Agostino Rocco
Published: January 2022

The ability of metabolomics to provide a snapshot of an individual’s metabolic state makes it a very useful technique in neonatology for investigating the complex relationship between nutrition and the state of health of the newborn. Through an 1H-NMR metabolomics analysis, we aimed to investigate the metabolic profile of newborns by analyzing both urine and milk samples in relation to the birth weight of neonates classified as AGA (adequate for the gestational age, n = 51), IUGR (intrauterine growth restriction, n = 14), and LGA (large for gestational age, n = 15). Samples were collected at 7 ± 2 days after delivery. Of these infants, 42 were exclusively breastfed, while 38 received mixed feeding with a variable amount of commercial infant formula (less than 40%) in addition to breast milk. We observed a urinary spectral pattern for oligosaccharides very close to that of the corresponding mother’s milk in the case of exclusively breastfed infants, thus mirroring the maternal phenotype. The absence of this good match between the infant urine and human milk spectra in the case of mixed-fed infants could be reasonably ascribed to the use of a variable amount of commercial infant formulas (under 40%) added to breast milk. Furthermore, our findings did not evidence any significant differences in the spectral profiles in terms of the neonatal customize centile, i.e., AGA (adequate for gestational age), LGA (large for gestational age), or IGUR (intrauterine growth restriction). It is reasonable to assume that maternal human milk oligosaccharide (HMO) production is not or is only minimally influenced by the fetal growth conditions for unknown reasons. This hypothesis may be supported by our metabolomics-based results, confirming once again the importance of this approach in the neonatal field.
Beneficial Effects of the Consumption of Hot-Water Extracts of Thinned Immature Mangos (Mangifera indica “Irwin”) on the Hypertriglyceridemia of Apolipoprotein E-Deficient Mice
Authors: Tajiri Hayato , Tanaka Wataru , Matsuyama Hiroki , Sugita Takuya , Hidaka Kenta , Yokoyama Daigo , Sakakibara Hiroyuki
Published: January 2022

The thinned immature fruit of the mango tree (Mangifera indica “Irwin”) are regarded as waste products. In this study, we evaluated the effects of daily consumption of a hot-water extract of thinned immature mango fruits (TIMEx) on the dyslipidemia of apolipoprotein E-deficient (ApoE−/−) mice. ApoE−/− mice and wild-type BALB/c mice were fed a 20% fat diet containing 0%, 0.1%, or 1.0% TIMEx for 8 weeks. Their body mass, food intake, and water consumption were unaffected by the TIMEx. The 1.0% TIMEx supplementation significantly reduced serum triglyceride, but not total cholesterol concentration. This effect was significant in ApoE−/− mice, but less marked under normal conditions in wild-type mice. In addition, the circulating concentrations of three hormones that regulate metabolism, resistin, leptin, and glucose-dependent insulinotropic polypeptide, were reduced by TIMEx consumption, which may be involved in its effect to prevent hypertriglyceridemia. However, none of the concentrations of TIMEx reduced the size of atherosclerotic plaque lesions. In conclusion, daily consumption of TIMEx ameliorates hypertriglyceridemia but not hypercholesterolemia in genetically predisposed mice.
Hepatitis B Virus Integration into Transcriptionally Active Loci and HBV-Associated Hepatocellular Carcinoma
Authors: Bousali Maria , Karamitros Timokratis
Published: January 2022

Hepatitis B Virus (HBV) DNA integrations into the human genome are considered major causative factors to HBV-associated hepatocellular carcinoma development. In the present study, we investigated whether HBV preferentially integrates parts of its genome in specific genes and evaluated the contribution of the integrations in HCC development per gene. We applied dedicated in-house developed pipelines on all of the available HBV DNA integration data and performed a statistical analysis to identify genes that could be characterized as hotspots of integrations, along with the evaluation of their association with HBV-HCC. Our results suggest that 15 genes are recurrently affected by HBV integrations and they are significantly associated with HBV-HCC. Further studies that focus on HBV integrations disrupting these genes are mandatory in order to understand the role of HBV integrations in clonal advantage gain and oncogenesis promotion, as well as to determine whether inhibition of the HBV-disrupted genes can provide a therapy strategy for HBV-HCC.
Structural and Metabolic Profiling of Lycopersicon esculentum Rhizosphere Microbiota Artificially Exposed at Commonly Used Non-Steroidal Anti-Inflammatory Drugs
Authors: Kovacs Emoke Dalma , Silaghi-Dumitrescu Luminita , Roman Cecilia , Tian Di
Published: January 2022

In this study, the effect of common non-steroidal anti-inflammatory drugs on Lycopersicon esculentum rhizosphere microbiota was monitored. The experiments were performed with artificially contaminated soil with ibuprofen (0.5 mg·kg−1), ketoprofen (0.2 mg·kg−1) and diclofenac (0.7 mg·kg−1). The results evidenced that the rhizosphere microbiota abundance decreased especially under exposure to diclofenac (187–201 nmol·g−1 dry weight soil) and ibuprofen (166–183 nmol·g−1 dry weight soil) if compared with control (185–240 nmol·g−1 dry weight soil), while the fungal/bacteria ratio changed significantly with exposure to diclofenac (<27%) and ketoprofen (<18%). Compared with control samples, the average amount of the ratio of Gram-negative/Gram-positive bacteria was higher in rhizosphere soil contaminated with ibuprofen (>25%) and lower in the case of diclofenac (<46%) contamination. Carbon source consumption increased with the time of assay in case of the control samples (23%) and those contaminated with diclofenac (8%). This suggests that rhizosphere microbiota under contamination with diclofenac consume a higher amount of carbon, but they do not consume a larger variety of its sources. In the case of contamination with ibuprofen and ketoprofen, the consumption of carbon source presents a decreasing tendency after day 30 of the assay. Rhizosphere microbiota emitting volatile organic compounds were also monitored. Volatile compounds belonging to alcohol, aromatic compounds, ketone, terpene, organic acids, aldehyde, sulphur compounds, esters, alkane, nitrogen compounds, alkene and furans were detected in rhizosphere soil samples. Among these, terpene, ketone, alcohol, aromatic compounds, organic acids and alkane were the most abundant compound classes (>75%), but their percentage changed with exposure to diclofenac, ketoprofen and ibuprofen. Such changes in abundance, structure and the metabolic activity of Lycopersicon esculentum rhizosphere microbiota under exposure to common non-steroidal anti-inflammatory drugs suggest that there is a probability to also change the ecosystem services provided by rhizosphere microbiota.
Novel Clinical and Laboratorial Challenges in Aspergillosis
Authors: Sabino Raquel , Veríssimo Cristina
Published: January 2022

In recent years, research in the areas of Aspergillus and aspergillosis has continued to advance rapidly, including advancements in genomics, immunological studies, clinical areas, and diagnostic areas. Recently, new risk groups for the development of aspergillosis have emerged—patients with influenza- or COVID-19-ssociated pulmonary aspergillosis. The rise and spread of antifungal resistances have also become a clinical concern in some geographic areas and have drawn the attention of clinicians due to difficulties in treating these infections. In this paper, a snapshot of these issues is presented, emphasizing these novel clinical and laboratorial challenges in the aspergillosis field and focusing on their actual relevance.
Microbial Consortia Are Needed to Degrade Soil Pollutants
Authors: Zhang Ting , Zhang Houjin
Published: January 2022

Soil pollution is one of the most serious environmental problems globally due to the weak self-purification ability, long degradation time, and high cost of cleaning soil pollution. The pollutants in the soil can be transported into the human body through water or dust, causing adverse effects on human health. The latest research has shown that the clean-up of soil pollutants through microbial consortium is a very promising method. This review provides an in-depth discussion on the efficient removal, bio-adsorption, or carbonated precipitation of organic and inorganic pollutants by the microbial consortium, including PAHs, BPS, BPF, crude oil, pyrene, DBP, DOP, TPHP, PHs, butane, DON, TC, Mn, and Cd. In view of the good degradation ability of the consortium compared to single strains, six different synergistic mechanisms and corresponding microorganisms are summarized. The microbial consortium obtains such activities through enhancing synergistic degradation, reducing the accumulation of intermediate products, generating the crude enzyme, and self-regulating, etc. Furthermore, the degradation efficiency of pollutants can be greatly improved by adding chemical materials such as the surfactants Tween 20, Tween 80, and SDS. This review provides insightful information regarding the application of microbial consortia for soil pollutant removal.
SARS-CoV-2 Serological and Biomolecular Analyses among Companion Animals in Campania Region (2020–2021)
Authors: Cardillo Lorena , de Martinis Claudio , Brandi Sergio , Levante Martina , Cozzolino Loredana , Spadari Luisa , Boccia Federica , Carbone Carmine , Pompameo Marina , Fusco Giovanna
Published: January 2022

The first reports of SARS-CoV-2 among domestic and wild animals, together with the rapid emergence of new variants, have created serious concerns regarding a possible spillback from animal hosts, which could accelerate the evolution of new viral strains. The present study aimed to investigate the prevalence and the transmission of SARS-CoV-2 among both owned and stray pets. A total of 182 dogs and 313 cats were tested for SARS-CoV-2. Specimens collected among owned and stray pets were subjected to RT-PCR and serological examinations. No viral RNA was detected, while anti-N antibodies were observed in six animals (1.3%), one dog (0.8%) and five cats (1.7%). Animals’ background revealed that owned cats, living with owners with COVID-19, showed significantly different prevalence compared to stray ones (p = 0.0067), while no difference was found among dogs. Among the seropositive pets, three owned cats also showed moderate neutralizing antibody titers. Pets and other species are susceptible to SARS-CoV-2 infection because of the spike affinity towards their ACE2 cellular receptor. Nevertheless, the risk of retransmission remains unclear since pet-to-human transmission has never been described. Due to the virus’ high mutation rate, new reservoirs cannot be excluded; thus, it is reasonable to test pets, mostly if living in households affected by COVID-19.
Impact of IFN-γ Deficiency on the Cardiomyocyte Function in the First Stage of Experimental Chagas Disease
Authors: Roman-Campos Danilo , Sales-Junior Policarpo , Costa Alexandre D. , Souza Diego Santos , Santos-Miranda Artur , Joviano-Santos Julliane V. , Ropert Catherine , Cruz Jader S.
Published: January 2022

Chagas disease (CD) is caused by the parasitic protozoan T. cruzi. The progression of CD in ~30% of patients results in Chagasic Cardiomyopathy (CCM). Currently, it is known that the inflammatory system plays a significant role in the CCM. Interferon-gamma (IFN-γ) is the major cytokine involved in parasitemia control but has also been linked to CCM. The L-type calcium current (ICa,L) is crucial in the excitation/contraction coupling in cardiomyocytes. Thus, we compared ICa,L and the mechanical properties of cardiomyocytes isolated from infected wild type (WT) and IFN-γ(−/−) mice in the first stage of T. cruzi infection. Using the patch clamp technique, we demonstrated that the infection attenuated ICa,L in isolated cardiomyocytes from the right and left ventricles of WT mice at 15 days post-infection (dpi), which was not observed in the IFN-γ(−/−) cardiomyocytes. However, ICa,L was attenuated between 26 and 30 dpi in both experimental groups. Interestingly, the same profile was observed in the context of the mechanical properties of isolated cardiomyocytes from both experimental groups. Simultaneously, we tracked the mortality and MCP-1, TNF-α, IL-12, IL-6, and IL-10 serum levels in the infected groups. Importantly, the IFN-γ(−/−) and WT mice presented similar parasitemia and serum inflammatory markers at 10 dpi, indicating that the modifications in the cardiomyocyte functions observed at 15 dpi were directly associated with IFN-γ(−/−) deficiency. Thus, we showed that IFN-γ plays a crucial role in the electromechanical remodeling of cardiomyocytes during experimental T. cruzi infection in mice.
Why Are Bifidobacteria Important for Infants?
Authors: Stuivenberg Gerrit A. , Burton Jeremy P. , Bron Peter A. , Reid Gregor
Published: January 2022

The presence of Bifidobacterium species in the maternal vaginal and fecal microbiota is arguably an evolutionary trait that allows these organisms to be primary colonizers of the newborn intestinal tract. Their ability to utilize human milk oligosaccharides fosters their establishment as core health-promoting organisms throughout life. A reduction in their abundance in infants has been shown to increase the prevalence of obesity, diabetes, metabolic disorder, and all-cause mortality later in life. Probiotic strains have been developed as supplements for premature babies and to counter some of these ailments as well as to confer a range of health benefits. The ability to modulate the immune response and produce short-chain fatty acids, particularly acetate and butyrate, that strengthen the gut barrier and regulate the gut microbiome, makes Bifidobacterium a core component of a healthy infant through adulthood.
Damage and Failure Characteristics of Surrounding Rock in Deep Circular Cavern under Cyclic Dynamic Load: A True Triaxial Experiment Investigation
Authors: Li Binglei , Gao Pengfei , Cao Yangbing , Gong Weiguo , Zhang Sui , Zhang Jianzhi
Published: January 2022

For ensuring safety and efficiency during the construction of deep engineering, it is essential to explore the failure mode of the surrounding rock mass under dynamic disturbance and high geo-stress. We conducted true triaxial load tests for rock-like material with a preexisting circular hole, and monitored the acoustic emission (AE) signal during the whole test. The result demonstrates the evolution characteristics of damage and failure mode with different cyclic dynamic load amplitudes and intermediate principal stress. With the increase in cyclic dynamic load amplitude or the decrease in intermediate principal stress, the failure source mainly occurs at the two horizontal side walls of the surrounding rock where the failure patterns change from the slabbing to wall caving and, finally, to rockburst. The former failure mode can actually serve as an important precursor for the latter. Based on such mechanisms, the precursor can be indirectly detected in forms of AE signal released by microcracking. The research can provide a reliable guidance for the rock stability control and faithfully forecasting the larger-scale failure during the excavation of deep circular cavern.
Comment on Volodichev et al. Archean Zircons with Omphacite Inclusions from Eclogites of the Belomorian Province, Fennoscandian Shield: The First Finding. Minerals 2021, 11, 1029
Authors: Skublov Sergey G. , Berezin Aleksey V. , Salimgaraeva Laysan I.
Published: January 2022

Volodichev et al. (Volodichev et al., 2021) reported on the first finding of omphacite (23%–25% Jd) inclusions in 2.68 Ga metamorphic zircons from Gridino eclogites and presented it as evidence for Archean eclogite-facies metamorphism in the Belomorian Mobile Belt. We believe that the Archean age of the garnets referred to by the above authors was estimated incorrectly. Our interpretation is that omphacite origin is related to Archean high-pressure granulite-facies metamorphism.
Numerical Simulation of a Single and Double-Rotor Impact Crusher Using Discrete Element Method
Authors: Bwalya Murray M. , Chimwani Ngonidzashe
Published: January 2022

The Discrete element method (DEM) is an invaluable tool for studying comminution as it provides detailed information that can help with process analysis as well as trying out new equipment designs before the equipment is physically built. The DEM was used to analyse previous experimental work to gain some insight into the comminution process in an impact crusher with a single impeller. Further DEM simulations were done on a crusher with a second impeller installed. The energy spectra and threshold energy levels calculated from the drop-weight test were used as the basis of comparison. The simulations indicate that even at much lower speeds, the performance of a double impeller impact crusher is exceedingly superior. However, the energy associated with the double impeller impact crusher is much higher and energy intensification, rather than energy efficiency, is the main gain of the double impeller design. The double impeller also offers more operational flexibility, such as spacing between the impellers, which can be tailored to the particle size range being handled.
Zircon (U-Th)/He Closure Temperature Lower Than Apatite Thermochronometric Systems: Reconciliation of a Paradox
Authors: Gérard Benjamin , Robert Xavier , Grujic Djordje , Gautheron Cécile , Audin Laurence , Bernet Matthias , Balvay Mélanie
Published: January 2022

Here, we present seven new zircon (U-Th)/He (ZHe) ages and three new zircon fission track (ZFT) ages analyzed from an age-elevation profile (Machu Picchu, Peru). ZFT data present ages older than those obtained with other thermochronological data, whereas the ZHe data interestingly present ages similar to those obtained with apatite (U-Th)/He (AHe). It has been proposed that He retention in zircon is linked to the damage dose, with an evolution of the closure temperature from low values associated with a low α-dose (<1016 α/g), subsequently increasing before decreasing again at a very high α-dose (>1018 α/g). Studies have focused on He diffusion behavior at high α-dose, but little is known at low doses. We propose that the ZHe closure temperature at α-dose ranging from 6 × 1015 to 4 × 1016 α/g is in the range of ~60–80 °C. This value is lower than that proposed in the current damage model ZRDAAM and demonstrates that the ZHe and AHe methods could have similar closure temperatures at low α-dose (i.e., similar ages). These new data strengthen our previous geological conclusions and even highlight a cooling rate approximately twice as important as that deduced from AHe and apatite fission track data alone at Machu Picchu.
The Influence of Hop Phenolic Compounds on Dry Hopping Beer Quality
Authors: Gribkova Irina N. , Kharlamova Larisa N. , Lazareva Irina V. , Zakharov Maxim A. , Zakharova Varvara A. , Kozlov Valery I.
Published: January 2022

Background: The article considers the phenolic hop compounds’ effect on the quality indicators of finished beer. The topic under consideration is relevant since it touches on the beer matrix colloidal stability when compounds with potential destabilizing activity are introduced into it from the outside. Methods: The industrial beer samples’ quality was assessed by industry-accepted methods and using instrumental analysis methods (high-performance liquid chromatography methods—HPLC). The obtained statistical data were processed by the Statistics program (Microsoft Corporation, Redmond, WA, USA, 2006). Results: The study made it possible to make assumptions about the functional dependence of the iso-α-bitter resins and isoxanthohumol content in beer samples. Mathematical analysis indicate interactions between protein molecules and different malted grain and hop compounds are involved in beer structure, in contrast to dry hopped beer, where iso-a-bitter resins, protein, and coloring compounds were significant, with a lower coefficient of determination. The main role of rutin in the descriptor hop bitterness has been established in kettle beer hopping technology, and catechin in dry beer hopping technology, respectively. The important role of soluble nitrogen and β-glucan dextrins in the perception of sensory descriptors of various technologies’ beers, as well as phenolic compounds in relation to the formation of bitterness and astringency of beer of classical technology and cold hopping, has been shown. Conclusions: The obtained mathematical relationships allow predicting the resulting beer quality and also make it possible to create the desired flavor profiles.
Study of Structural and Optical Properties of Electrodeposited Silicon Films on Graphite Substrates
Authors: Islam Muhammad Monirul , Said Hajer , Hamzaoui Ahmed Hichem , Mnif Adel , Sakurai Takeaki , Fukata Naoki , Akimoto Katsuhiro
Published: January 2022

Silicon (Si) films were deposited on low-cost graphite substrates by the electrochemical reduction of silicon dioxide nanoparticles (nano-SiO2) in calcium chloride (CaCl2), melted at 855 °C. Cyclic voltammetry (CV) was used to analyze the electrochemical reduction mechanism of SiO2 to form Si deposits on the graphite substrate. X-ray diffraction (XRD) along with Raman and photoluminescence (PL) results show that the crystallinity of the electrodeposited Si-films was improved with an increase of the applied reduction potential during the electrochemical process. Scanning electron microscopy (SEM) reveals that the size, shape, and morphology of the Si-layers can be controlled from Si nanowires to the microcrystalline Si particles by controlling the reduction potentials. In addition, the morphology of the obtained Si-layers seems to be correlated with both the substrate materials and particle size of the feed materials. Thus, the difference in the electron transfer rate at substrate/nano-SiO2 interface due to different applied reduction potentials along with the dissolution rate of SiO2 particles during the electrochemical reduction process were found to be crucial in determining the microstructural properties of the Si-films.