bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigate the Equivalence of Neutrons and Protons in Single Event Effects Testing: A Geant4 Study
Authors: Chiang Yueh , Tan Cher Ming , Chao Tsi-Chian , Lee Chung-Chi , Tung Chuan-Jong
Published: May 2020

Neutron radiation on advanced integrated circuits (ICs) is becoming important for their reliable operation. However, a neutron test on ICs is expensive and time-consuming. In this work, we employ Monte Carlo simulation to examine if a proton test can replace or even accelerate the neutron test, and we found that 200 MeV protons are the closest to resembling neutron radiation with five main differences. This 200 MeV concur with the suggestion from National Aeronautics and Space Administration (NASA, Washington, DC, USA). However, the impacts of the five differences on single event effects (SEEs) require future work for examination.
Special Issue: Movement Biomechanics and Motor Control
Authors: Frigo Carlo Albino
Published: May 2020

When Applied Science invited me to organize a Special Issue on Movement Biomechanics and Motor Control, more than one year ago, I was surprised, but also flattered by the invitation [...]
Construction of Ag3PO4/SnO2 Heterojunction on Carbon Cloth with Enhanced Visible Light Photocatalytic Degradation
Authors: Liu Min , Wang Guangxin , Xu Panpan , Zhu Yanfeng , Li Wuhui
Published: May 2020

In this study, the Ag3PO4/SnO2 heterojunction on carbon cloth (Ag3PO4/SnO2/CC) was successfully fabricated via a facile two-step process. The results showed that the Ag3PO4/SnO2/CC heterojunction exhibited a remarkable photocatalytic performance for the degradation of Rhodamine B (RhB) and methylene blue (MB), under visible light irradiation. The calculated k values for the degradation of RhB and MB over Ag3PO4/SnO2/CC are 0.04716 min−1 and 0.04916 min−1, which are higher than those calculated for the reactions over Ag3PO4/SnO2, Ag3PO4/CC and SnO2/CC, respectively. The enhanced photocatalytic activity could mainly be attributed to the improved separation efficiency of photogenerated electron-hole pairs, after the formation of the Ag3PO4/SnO2/CC heterojunction. Moreover, carbon cloth with a large specific surface area and excellent conductivity was used as the substrate, which helped to increase the contact area of dye solution with photocatalysts and the rapid transfer of photogenerated electrons. Notably, when compared with the powder catalyst, the catalysts supported on carbon cloth are easier to quickly recycle from the pollutant solution, thereby reducing the probability of recontamination.
Removal of Arsenic, Chromium and Uranium from Water Sources by Novel Nanostructured Materials Including Graphene-Based Modified Adsorbents: A Mini Review of Recent Developments
Authors: Tolkou Athanasia K. , Katsoyiannis Ioannis A. , Zouboulis Anastasios I.
Published: May 2020

Groundwater is commonly used as a drinking water resource all over the world. Therefore, groundwater contamination by toxic metals is an important issue of utmost concern for public health, and several technologies are applied for their effective removal, such as coagulation, ion exchange, adsorption, and membrane applications like reverse osmosis. Adsorption is acknowledged as a simple, effective and economic technology, which has received increased interest recently, despite certain limitations regarding operational applications. The respective scientific efforts have been specifically focused on the development and implementation of novel nano-structured adsorbent materials, which may offer extensive specific surface areas, much higher than the conventional adsorbents, and hence, are expected to present higher removal efficiencies of pollutants. In this paper, the recent developments of nanomaterial applications for arsenic, chromium and uranium removal from groundwaters are critically reviewed. Particularly, the use of novel composite materials, based mainly on hybrid metallic oxide nanoparticles and on composites based on graphene oxide (GO) (i.e., graphene-based hybrids), showed promising evidences to achieve efficient removal of toxic metals from water sources, even in full scale applications.
Study on Passenger Comfort Based on Human–Bus–Road Coupled Vibration
Authors: Wang Guichun , Zhang Jie , Kong Xuan
Published: May 2020

Nowadays, the comfort of drivers and passengers is drawing more and more attention. The interaction between vehicles and the asphalt road can cause coupled vibration and reduce the comfort of passengers. Therefore, the research on human–vehicle–road coupled vibration and the comfort of passengers is of great importance. In this paper, the three-dimensional human–bus–road coupled vibration system is established, including the bus model, the parallel biomechanical human model with 2 degrees-of-freedom (DOF), and road surface roughness condition. The proposed coupled model was then used to study the dynamic response of the system and the comfort evaluation of the human body. In the comfort evaluation, the annoyance rate based method was proposed to consider the randomness of passenger vibration, the difference of the psychosensory vibration, and the fuzziness of evaluation indicators. Compared to the fuzzy evaluation based on the ISO 2631 standard, the proposed annoyance rate based method gives a quantitative evaluation of human comfort. Not only the degree of comfort can be evaluated, but the percentage of people feeling uncomfortable can also be obtained. Finally, parametric studies were conducted to investigate the effects of road surface roughness, interlayer bonding condition, bus weight, bus speed, and sitting position. It is found that the road surface roughness has the most significant effect on human comfort.
The Effect of Pesticides on the Tomato Bacterial Speck Disease Pathogen Pseudomonas Syringae pv. Tomato
Authors: Butsenko Liudmyla , Pasichnyk Lidiia , Kolomiiets Yuliia , Kalinichenko Antonina
Published: May 2020

A significant part of the used pesticides does not reach the target organisms and, while remaining in the agrophytocenosis, influences all living organisms in it. Having a toxic and often mutagenic effect, pesticides induce morphological and physiological changes in the cells of microorganisms and are the cause of phenotypic heterogeneity of their populations. However, the effect of pesticides on phytopathogenic bacteria as non-target microorganisms remains out of the field of view for most researchers. However, the use of pesticides can lead to expansion of the diversity of existing phytopathogens and, as a consequence, complications of identification of the pathogens, loss of resistance by plants varieties, and increased harm from diseases caused by them. This study is focused on the effect of pesticides used in tomato plantations on the causative agent of bacterial speck of this crop—Pseudomonas syringae pv. tomato. The studies were carried out using the methods of classical microbiology. The mutagenic action of pesticides was recorded, taking into account the increase of the number of streptomycin resistance mutations in bacteria in the case of pesticide action. It is established that the fungicide aluminium phosethyl is characterised by a bacteriostatic effect on P. syringae pv. tomato. Deltamethrin insecticide does not affect the growth of P. syringae pv. tomato. However, there is an increase in the frequency of streptomycin resistance mutations in both studied strains of P. syringae pv. tomato after using deltamethrin. It is shown that the frequency of occurrence of R (rough colonies) forms of P. syringae pv. tomato IZ28 and IZ46 after using deltamethrin increased by 100 times when in comparison to the frequency of spontaneous morphological dissociation, or smooth-to-rough (S-R) mutation, of these bacteria. Therefore, aluminium phosethyl is characterised by moderate bacteriostatic action against P. syringae pv. tomato. Deltamethrin does not influence the growth of the pathogen of tomato speck but increases the frequency of formation of StrR mutants and R forms of phytopathogenic bacteria.
Distribution of Magnetic Field in 400 kV Double-Circuit Transmission Lines
Authors: Deltuva Ramūnas , Lukočius Robertas
Published: May 2020

A high-voltage AC double-circuit 400 kV overhead power transmission line runs from the city of Elk (Poland) to the city of Alytus (Lithuania). This international 400 kV power transmission line is potentially one of the strongest magnetic field-generating sources in the area. This 400 kV voltage double-circuit overhead transmission line and its surroundings were analyzed using the mathematical analytical methods of superposition and reflections. This research paper includes the calculation of the numerical values of the magnetic field and its distribution. The research showed that the values of the magnetic field strength near the international 400 kV power transmission line exceed the threshold values permitted by relevant standards. This overhead power line is connected to the general (50 Hz) power system and generates a highly intense magnetic field. It is suggested that experimental trials should be undertaken in order to determine the maximum values of the magnetic field strength. For the purpose of mitigating these values, it is suggested that the height of the support bars should be increased or that any individual and commercial activities near the object under investigation should be restricted.
Compensation of Measurement Uncertainty in a Remote Fetal Monitor
Authors: Arciniega-Montiel Sadot , Ronquillo-Lomeli Guillermo , Salas-Zúñiga Roberto , Salgado-Jiménez Tomás , Barriga-Rodríguez Leonardo
Published: May 2020

The perinatal mortality rate is very high throughout the world. A fetal monitor may be used remotely, and this would tackle the problem of continuous monitoring of high-risk pregnancies. There is evidence that current technology is of low reliability, and, therefore, of low precision to identify fetal health. In medical technological implementation, a safe, efficient, and reliable operation must be guaranteed, and the main problem is that remote fetal monitor gathers just a few samples, so the hypothesis of classical theory is not met. We are proposing an approach that improves the data’s lack of reliability that accompanies the use of a remote fetal monitor. The method refers to how, by using the existing technologies and the initial experimental data, it is possible to apply probabilistic models that are truly representative of each application. This leads to the characterization of properties of the statistics used to generate a representative probabilistic model without the need to consider the hard suppositions. Results show that, for every case study, it was possible to improve estimations of measurement uncertainty. The proposed method is a useful tool to increase the reliability of medical technology, especially for pieces of equipment where a health care professional is not available.
Individual Topological Analysis of Synchronization-Based Brain Connectivity
Authors: Lombardi Angela , Amoroso Nicola , Diacono Domenico , Monaco Alfonso , Tangaro Sabina , Bellotti Roberto
Published: May 2020

Functional connectivity analysis aims at assessing the strength of functional coupling between the signal responses in distinct brain areas. Usually, functional magnetic resonance imaging (fMRI) time series connections are estimated through zero-lag correlation metrics that quantify the statistical similarity between pairs of regions or spectral measures that assess synchronization at a frequency band of interest. Here, we explored the application of a new metric to assess the functional synchronization in phase space between fMRI time series in a resting state. We applied a complete topological analysis to the resulting connectivity matrix to uncover both the macro-scale organization of the brain and detect the most important nodes. The synchronization metric is also compared with Pearson’s correlation coefficient and spectral coherence to highlight similarities and differences between the topologies of the three functional networks. We found that the individual topological organization of the resulting synchronization-based connectivity networks shows a finer modular organization than that identified with the other two metrics and a low overlap with the modular partitions of the other two networks suggesting that the derived topological information is not redundant and could be potentially integrated to provide a multi-scale description of functional connectivity.
Numerical Study of Multilayer Planar Film Structures for Ideal Absorption in the Entire Solar Spectrum
Authors: Chen Wei , Liu Jing , Ma Wen-Zhuang , Yu Gao-Xiang , Chen Jing-Qian , Cai Hao-Yuan , Yang Cheng-Fu
Published: May 2020

Here, we have theoretically proposed an ideal structure of selective solar absorber with multilayer planar films, which can absorb the incident light throughout the entire solar spectrum (300–2500 nm) and over a wide angular range, whatever the polarization angle of 0°~90°. The efficiency of the proposed absorber is proven by the Finite-Difference Time Domain (FDTD) simulation. The average absorption rate over the solar spectrum is up to 96.6%. The planar design is extremely easy to fabricate and modify, and this structure does not require lithographic processes to finish the absorbers. Improvements of the solar absorber on the basis of planar multilayer-film structures is attributed to multiple asymmetric highly lossy Fabry–Perot resonators. Because of having many virtues, such as using different refractory and non-noble metals, having angle and polarization independence, and having ideal absorption for entire solar spectrum, our proposed absorbers are promising candidates for practical industrial production of the solar-energy harvesting.
Investigations of Object Detection in Images/Videos Using Various Deep Learning Techniques and Embedded Platforms—A Comprehensive Review
Authors: Murthy Chinthakindi Balaram , Hashmi Mohammad Farukh , Bokde Neeraj Dhanraj , Geem Zong Woo
Published: May 2020

In recent years there has been remarkable progress in one computer vision application area: object detection. One of the most challenging and fundamental problems in object detection is locating a specific object from the multiple objects present in a scene. Earlier traditional detection methods were used for detecting the objects with the introduction of convolutional neural networks. From 2012 onward, deep learning-based techniques were used for feature extraction, and that led to remarkable breakthroughs in this area. This paper shows a detailed survey on recent advancements and achievements in object detection using various deep learning techniques. Several topics have been included, such as Viola–Jones (VJ), histogram of oriented gradient (HOG), one-shot and two-shot detectors, benchmark datasets, evaluation metrics, speed-up techniques, and current state-of-art object detectors. Detailed discussions on some important applications in object detection areas, including pedestrian detection, crowd detection, and real-time object detection on Gpu-based embedded systems have been presented. At last, we conclude by identifying promising future directions.
Fast Simulation of Laser Heating Processes on Thin Metal Plates with FFT Using CPU/GPU Hardware
Authors: Mejia-Parra Daniel , Arbelaiz Ander , Ruiz-Salguero Oscar , Lalinde-Pulido Juan , Moreno Aitor , Posada Jorge
Published: May 2020

In flexible manufacturing systems, fast feedback from simulation solutions is required for effective tool path planning and parameter optimization. In the particular sub-domain of laser heating/cutting of thin rectangular plates, current state-of-the-art methods include frequency-domain (spectral) analytic solutions that greatly reduce the required computational time in comparison to industry standard finite element based approaches. However, these spectral solutions have not been presented previously in terms of Fourier methods and Fast Fourier Transform (FFT) implementations. This manuscript presents four different schemes that translate the problem of laser heating of rectangular plates into equivalent FFT problems. The presented schemes make use of the FFT algorithm to reduce the computational time complexity of the problem from O ( M 2 N 2 ) to O ( M N log ( M N ) ) (with M × N being the discretization size of the plate). The test results show that the implemented schemes outperform previous non-FFT approaches both in CPU and GPU hardware, resulting in 100 × faster runs. Future work addresses thermal/stress analysis, non-rectangular geometries and non-linear interactions (such as material melting/ablation, convection and radiation heat transfer).
SHS Produced TiB2-Si Powders for Selective Laser Melting of Ceramic-Based Composite
Authors: Liu Le , Aydinyan Sofiya , Minasyan Tatevik , Hussainova Irina
Published: May 2020

One of the main limitations for widespread additive manufacturing is availability and processability of the precursor materials feedstock. For the first time, this study reports the development of a “pomegranate-like” structured TiB2-Si ceramic-metalloid powder feedstock suitable for selective laser melting (SLM) of ceramic-based composite. The powder was produced via self-propagating high temperature synthesis (SHS) at a moderate combustion temperature of 1530 °C. The effective activation energy in the Ti-B-Si system for the slow step of the combustion process was estimated as 184 kJ. Conditions of SHS process demonstrated a strong influence on the properties of produced powders and, therefore, on SLM parameters and properties of the printed materials. The powders have demonstrated a high performance for manufacturing bulks of 56 wt%TiB2–44 wt%Si ceramic-based composite.
Towards a Better Representation of Fog Microphysics in Large-Eddy Simulations Based on an Embedded Lagrangian Cloud Model
Authors: Schwenkel Johannes , Maronga Björn
Published: May 2020

The development of radiation fog is influenced by multiple physical processes such as radiative cooling and heating, turbulent mixing, and microphysics, which interact on different spatial and temporal scales with one another. Once a fog layer has formed, the number of fog droplets and their size distribution have a particularly large impact on the development of the fog layer due to their feedback on gravitational settling and radiative cooling at the fog top, which are key processes for fog. However, most models do not represent microphysical processes explicitly, or parameterize them rather crudely. In this study we simulate a deep radiation fog case with a coupled large-eddy simulation (LES)–Lagrangian cloud model (LCM) approach for the first time. By simulating several hundred million fog droplets as Lagrangian particles explicitly (using the so-called superdroplet approach), we include a size-resolved diffusional growth including Köhler theory and gravitational sedimentation representation. The results are compared against simulations using a state of the art bulk microphysics model (BCM). We simulate two different aerosol backgrounds (pristine and polluted) with each microphysics scheme. The simulations show that both schemes generally capture the key features of the deep fog event, but also that there are significant differences: the drop size distribution produced by the LCM is broader during the formation and dissipation phase than in the BCM. The LCM simulations suggest that its spectral shape, which is fixed in BCMs, exhibits distinct changes during the fog life cycle, which cannot be taken into account in BCMs. The picture of the overall fog droplet number concentration is twofold: For both aerosol environments, the LCM shows lower concentrations of larger fog droplets, while we observe a higher number of small droplets and swollen aerosols reducing the visibility earlier than in the BCM. As a result of the different model formulation we observe higher sedimentation rates and lower liquid water paths for the LCM. The present work demonstrates that it is possible to simulate fog with the computational demanding approach of LCMs to assess the advantages of high-resolution cloud models and further to estimate errors of traditional parameterizations.
Total p-PAH Levels Nearby a Complex Industrial Area: A Tailored Monitoring Experiment to Assess the Impact of Emission Sources
Authors: Di Gilio Alessia , Palmisani Jolanda , Trizio Livia , Saracino Gaetano , Giua Roberto , de Gennaro Gianluigi
Published: May 2020

In this study, data on the hourly concentrations of the total particle-bound Polycyclic Aromatic Hydrocarbons (p-PAHs) collected between 1 August 2013 and 31 August 2014 by the air quality fence monitoring network of the biggest European steel plant, were analyzed. In contrast with what was predicted, the total p-PAH concentration did not decrease with distance from the steel plant, and higher concentrations were registered at the Orsini site, in the urban settlement, relative to the Parchi site, which is nearest to the coke ovens. Therefore, in order to identify and explain the cause of these high concentrations, a tailored monitoring experiment was carried out on a specific monitoring pathway by using a total p-PAHs monitor placed onto a cart. The real-time monitoring of the total p-PAH concentration on the road revealed to be a useful tool, which identified vehicular traffic as an important source of p-PAHs and highlighted the possible high short-term effect that vehicular traffic sources could have on the health of the exposed human population. Moreover, the study focused attention on the importance of the spatial representativeness of fixed monitoring stations, especially in a highly complex industrial area such as Taranto (Southern Italy).
Anti-Atherosclerotic Activity of (3R)-5-Hydroxymellein from an Endophytic Fungus Neofusicoccum parvum JS-0968 Derived from Vitex rotundifolia through the Inhibition of Lipoproteins Oxidation and Foam Cell Formation
Authors: Kim Jae-Yong , Kim Soonok , Shim Sang Hee
Published: May 2020

An endophytic fungus, Neofusicoccum parvum JS-0968, was isolated from a plant, Vitex rotundifolia. The chemical investigation of its cultures led to the isolation of a secondary metabolite, (3R)-5-hydroxymellein. It has been reported to have antifungal, antibacterial, and antioxidant activity, but there have been no previous reports on the effects of (3R)-5-hydroxymellein on atherosclerosis. The oxidation of lipoproteins and foam cell formation have been known to be significant in the development of atherosclerosis. Therefore, we investigated the inhibitory effects of (3R)-5-hydroxymellein on atherosclerosis through low-density lipoprotein (LDL) and high-density lipoprotein (HDL) oxidation and macrophage foam cell formation. LDL and HDL oxidation were determined by measuring the production of conjugated dienes and malondialdehyde, the amount of hyperchromicity and carbonyl content, conformational changes, and anti-LDL oxidation. In addition, the inhibition of foam cell formation was measured by Oil red O staining. As a result, (3R)-5-hydroxymellein suppressed the oxidation of LDL and HDL through the inhibition of lipid peroxidation, the decrease of negative charges, the reduction of hyperchromicity and carbonyl contents, and the prevention of apolipoprotein A-I (ApoA-I) aggregation and apoB-100 fragmentation. Furthermore, (3R)-5-hydroxymellein significantly reduced foam cell formation induced by oxidized LDL (oxLDL). Taken together, our data show that (3R)-5-hydroxymellein could be a potential preventive agent for atherosclerosis via obvious anti-LDL and HDL oxidation and the inhibition of foam cell formation.
Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta
Authors: Du Yuzhe , Grodowitz Michael J. , Chen Jian
Published: May 2020

Contact and fumigation toxicity of four isothiocyanates (ITCs), including allyl isothiocyanate (AITC), 3-butenyl isothiocyanate (3BITC), 3-(methylthio) propyl isothiocyanate (3MPITC) and 2-phenylethyl isothiocyanate (2PEITC), were evaluated against the red imported fire ant worker, Solenopsis invicta Buren. 2PEITC and 3MPITC exhibited strong contact toxicity. The median lethal dose (LD50)value of AITC, 2PEITC and 3MPITC were 7.99, 2.36 and 2.09 µg/ant respectively. In addition, AITC and 3MPITC also showed strong fumigation toxicity but not 2PEITC. The median lethal concentration (LC50) values of AITC and 3MPITC were 32.49 and 57.6 µg/L, respectively. In contrast, 3BITC did not exhibit any contact and fumigation toxicity even at 100 μg/μL. Esterase (EST), glutathione S-transferase (GST) and acetylcholinesterase (AChE)-inhibiting activities were assessed for three ITCs in S. invicta workers. All three ITCs inhibited both EST and GST activities but not AChE. The in vitro half maximal inhibitory concentration (IC50)values of AITC, 2PEITC and 3MPITC for GST were 3.32, 0.61 and 0.66 µg/µL, respectively. These results suggested that naturally occurring ITCs might be potentially useful for developing fire ants control products.
Challenges in Matrix Metalloproteinases Inhibition
Authors: Laronha Helena , Carpinteiro Inês , Portugal Jaime , Azul Ana , Polido Mário , Petrova Krasimira T. , Salema-Oom Madalena , Caldeira Jorge
Published: May 2020

Matrix metalloproteinases are enzymes that degrade the extracellular matrix. They have different substrates but similar structural organization. Matrix metalloproteinases are involved in many physiological and pathological processes and there is a need to develop inhibitors for these enzymes in order to modulate the degradation of the extracellular matrix (ECM). There exist two classes of inhibitors: endogenous and synthetics. The development of synthetic inhibitors remains a great challenge due to the low selectivity and specificity, side effects in clinical trials, and instability. An extensive review of currently reported synthetic inhibitors and description of their properties is presented.
Genome-Wide Open Chromatin Methylome Profiles in Colorectal Cancer
Authors: Ishak Muhiddin , Baharudin Rashidah , Mohamed Rose Isa , Sagap Ismail , Mazlan Luqman , Mohd Azman Zairul Azwan , Abu Nadiah , Jamal Rahman , Lee Learn-Han , Ab Mutalib Nurul Syakima
Published: May 2020

The methylome of open chromatins was investigated in colorectal cancer (CRC) to explore cancer-specific methylation and potential biomarkers. Epigenome-wide methylome of open chromatins was studied in colorectal cancer tissues using the Infinium DNA MethylationEPIC assay. Differentially methylated regions were identified using the ChAMP Bioconductor. Our stringent analysis led to the discovery of 2187 significant differentially methylated open chromatins in CRCs. More hypomethylated probes were observed and the trend was similar across all chromosomes. The majority of hyper- and hypomethylated probes in open chromatin were in chromosome 1. Our unsupervised hierarchical clustering analysis showed that 40 significant differentially methylated open chromatins were able to segregate CRC from normal colonic tissues. Receiver operating characteristic analyses from the top 40 probes revealed several significant, highly discriminative, specific and sensitive probes such as OPLAH cg26256223, EYA4 cg01328892, and CCNA1 cg11513637, among others. OPLAH cg26256223 hypermethylation is associated with reduced gene expression in the CRC. This study reports many open chromatin loci with novel differential methylation statuses, some of which with the potential as candidate markers for diagnostic purposes.
Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace
Authors: Ramirez Maria Soledad , Bonomo Robert A. , Tolmasky Marcelo E.
Published: May 2020

Acinetobacter baumannii is a common cause of serious nosocomial infections. Although community-acquired infections are observed, the vast majority occur in people with preexisting comorbidities. A. baumannii emerged as a problematic pathogen in the 1980s when an increase in virulence, difficulty in treatment due to drug resistance, and opportunities for infection turned it into one of the most important threats to human health. Some of the clinical manifestations of A. baumannii nosocomial infection are pneumonia; bloodstream infections; lower respiratory tract, urinary tract, and wound infections; burn infections; skin and soft tissue infections (including necrotizing fasciitis); meningitis; osteomyelitis; and endocarditis. A. baumannii has an extraordinary genetic plasticity that results in a high capacity to acquire antimicrobial resistance traits. In particular, acquisition of resistance to carbapenems, which are among the antimicrobials of last resort for treatment of multidrug infections, is increasing among A. baumannii strains compounding the problem of nosocomial infections caused by this pathogen. It is not uncommon to find multidrug-resistant (MDR, resistance to at least three classes of antimicrobials), extensively drug-resistant (XDR, MDR plus resistance to carbapenems), and pan-drug-resistant (PDR, XDR plus resistance to polymyxins) nosocomial isolates that are hard to treat with the currently available drugs. In this article we review the acquired resistance to carbapenems by A. baumannii. We describe the enzymes within the OXA, NDM, VIM, IMP, and KPC groups of carbapenemases and the coding genes found in A. baumannii clinical isolates.
The Effect of a Fast-Releasing Hydrogen Sulfide Donor on Vascularization of Subcutaneous Scaffolds in Immunocompetent and Immunocompromised Mice
Authors: Smink Alexandra M. , Najdahmadi Avid , Alexander Michael , Li Shiri , Rodriquez Samuel , van Goor Harry , Hillebrands Jan-Luuk , Botvinick Elliot , Lakey Jonathan R. T. , de Vos Paul
Published: May 2020

Islet transplantation into subcutaneous polymer scaffolds has shown to successfully induce normoglycemia in type 1 diabetes models. Vascularization of these scaffolds is imperative for optimal control of glucose levels. We studied the effect of the vascular stimulator hydrogen sulfide (H2S) on the degree of vascularization of a scaffold and the role of the immune system in this process. Scaffolds were subcutaneously implanted in immunocompetent C57BL/6 and immunocompromised nude mice. Mice received twice-daily intraperitoneal injections of the fast-releasing H2S donor sodium hydrosulfide (NaHS, 25 or 50 μmol/kg) or saline for 28 days. After 63 days the vascular network was analyzed by histology and gene expression. Here we showed that the vascularization of a subcutaneous scaffold in nude mice was significantly impaired by H2S treatment. Both the CD31 gene and protein expression were reduced in these scaffolds compared to the saline-treated controls. In C57BL/6 mice, the opposite was found, the vascularization of the scaffold was significantly increased by H2S. The mRNA expression of the angiogenesis marker CD105 was significantly increased compared to the controls as well as the number of CD31 positive blood vessels. In conclusion, the immune system plays an important role in the H2S mediated effect on vascularization of subcutaneous scaffolds.
Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity
Authors: de Carvalho Felipe Mendes de Andrade , Schneider Jaderson Kleveston , de Jesus Carla Viviane Freitas , de Andrade Luciana Nalone , Amaral Ricardo Guimarães , David Jorge Maurício , Krause Laíza Canielas , Severino Patrícia , Soares Cleide Mara Faria , Caramão Bastos Elina , Padilha Francine Ferreira , Gomes Silvana Vieira Flores , Capasso Raffaele , Santini Antonello , Souto Eliana Barbosa , de Albuquerque-Júnior Ricardo Luiz Cavalcanti
Published: May 2020

Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).
Expression of SCD and FADS2 Is Lower in the Necrotic Core and Growing Tumor Area than in the Peritumoral Area of Glioblastoma Multiforme
Authors: Korbecki Jan , Kojder Klaudyna , Jeżewski Dariusz , Simińska Donata , Tarnowski Maciej , Kopytko Patrycja , Safranow Krzysztof , Gutowska Izabela , Goschorska Marta , Kolasa-Wołosiuk Agnieszka , Wiszniewska Barbara , Chlubek Dariusz , Baranowska-Bosiacka Irena
Published: May 2020

The expression of desaturases is higher in many types of cancer, and despite their recognized role in oncogenesis, there has been no research on the expression of desaturases in glioblastoma multiforme (GBM). Tumor tissue samples were collected during surgery from 28 patients (16 men and 12 women) diagnosed with GBM. The effect of necrotic conditions and nutritional deficiency (mimicking conditions in the studied tumor zones) was studied in an in vitro culture of human brain (glioblastoma astrocytoma) U-87 MG cells. Analysis of desaturase expression was made by qRT-PCR and the immunohistochemistry method. In the tumor, the expression of stearoyl–coenzyme A desaturase (SCD) and fatty acid desaturases 2 (FADS2) was lower than in the peritumoral area. The expression of other desaturases did not differ in between the distinguished zones. We found no differences in the expression of SCD, fatty acid desaturases 1 (FADS1), or FADS2 between the sexes. Necrotic conditions and nutritional deficiency increased the expression of the studied desaturase in human brain (glioblastoma astrocytoma) U-87 MG cells. The obtained results suggest that (i) biosynthesis of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) in a GBM tumor is less intense than in the peritumoral area; (ii) expressions of SCD, SCD5, FADS1, and FADS2 correlate with each other in the necrotic core, growing tumor area, and peritumoral area; (iii) expressions of desaturases in a GBM tumor do not differ between the sexes; and (iv) nutritional deficiency increases the biosynthesis of MUFA and PUFA in GBM cells.
Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae
Authors: Khonsari Bahar , Klassen Roland
Published: May 2020

Pus1-dependent pseudouridylation occurs in many tRNAs and at multiple positions, yet the functional impact of this modification is incompletely understood. We analyzed the consequences of PUS1 deletion on the essential decoding of CAG (Gln) codons by tRNAGlnCUG in yeast. Synthetic lethality was observed upon combining the modification defect with destabilized variants of tRNAGlnCUG, pointing to a severe CAG-decoding defect of the hypomodified tRNA. In addition, we demonstrated that misreading of UAG stop codons by a tRNAGlnCUG variant is positively affected by Pus1. Genetic approaches further indicated that mildly elevated temperature decreases the decoding efficiency of CAG and UAG via destabilized tRNAGlnCAG variants. We also determined the misreading of CGC (Arg) codons by tRNAHisGUG, where the CGC decoder tRNAArgICG contains Pus1-dependent pseudouridine, but not the mistranslating tRNAHis. We found that the absence of Pus1 increased CGC misreading by tRNAHis, demonstrating a positive role of the modification in the competition against non-synonymous near-cognate tRNA. Part of the in vivo decoding defects and phenotypes in pus1 mutants and strains carrying destabilized tRNAGlnCAG were suppressible by additional deletion of the rapid tRNA decay (RTD)-relevant MET22, suggesting the involvement of RTD-mediated tRNA destabilization.
Smell—Adding a New Dimension to Urinalysis
Authors: Visser Eva H. , Berkhout Daan J. C. , Singh Jiwanjot , Vermeulen Annemieke , Ashtiani Niloufar , de Boer Nanne K. , van Wijk Joanna A. E. , de Meij Tim G. , Bökenkamp Arend
Published: May 2020

Background: Urinary tract infections (UTI) are among the most common infections in children. The primary tool to detect UTI is dipstick urinalysis; however, this has limited sensitivity and specificity. Therefore, urine culture has to be performed to confirm a UTI. Urinary volatile organic compounds (VOC) may serve as potential biomarker for diagnosing UTI. Previous studies on urinary VOCs focused on detection of UTI in a general population; therefore, this proof-of-principle study was set up in a clinical high-risk pediatric population. Methods: This study was performed at a tertiary nephro-urological clinic. Patients included were 0–18 years, clinically suspected of a UTI, and had abnormal urinalysis. Urine samples were divided into four groups, i.e., urine without bacterial growth, contamination, colonization, and UTI. VOC analysis was performed using an electronic nose (eNose) (Cyranose 320®) and VOC profiles of subgroups were compared. Results: Urinary VOC analysis discriminated between UTI and non-UTI samples (AUC 0.70; p = 0.048; sensitivity 0.67, specificity 0.70). The diagnostic accuracy of VOCs improved when comparing urine without bacterial growth versus with UTI (AUC 0.80; p = 0.009, sensitivity 0.79, specificity 0.75). Conclusions: In an intention-to-diagnose high-risk pediatric population, UTI could be discriminated from non-UTI by VOC profiling, using an eNose. Since eNose can be used as bed-side test, these results suggest that urinary VOC analysis may serve as an adjuvant in the diagnostic work-up of UTI in children.
Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput
Authors: He Limin , Sang Benliang , Wu Wenming
Published: May 2020

The traditional qPCR instrument is bulky, expensive, and inconvenient to carry, so we report a portable rotary real-time fluorescent PCR (polymerase chain reaction) that completes the PCR amplification of DNA in the field, and the reaction can be observed in real-time. Through the analysis of a target gene, namely pGEM-3Zf (+), the gradient amplification and melting curves are compared to commercial devices. The results confirm the stability of our device. This is the first use of a mechanical rotary structure to achieve gradient amplification curves and melting curves comparable to commercial instruments. The average power consumption of our system is about 7.6 W, which is the lowest energy consumption for real-time fluorescence quantification in shunting PCR and enables the use of our device in the field thanks to its self-contained power supply based on a lithium battery. In addition, all of the equipment costs only about 710 dollars, which is far lower than the cost of a commercial PCR instrument because the control system through mechanical displacement replaces the traditional TEC (thermoelectric cooler) temperature control. Moreover, the equipment has a low technical barrier, which can suit the needs of non-professional settings, with strong repeatability.
The Structural Performance of Reinforced Concrete Members with Monolithic Non-Structural Walls under Static and Dynamic Loads
Authors: Safi Walid Ahmad , Hibino Yo , Kusunoki Koichi , Mukai Tomohisa , Sanada Yasushi , Nakamura Izumi , Fukai Satoru
Published: May 2020

The required base shear and drift limit for post-disaster management buildings have increased in the Japanese Building Code following major seismic events. One method to satisfy these requirements for reinforced concrete frame buildings is to cast exterior non-structural concrete wall elements to be monolithic with frame elements, but without anchoring the longitudinal wall reinforcing. This provides additional stiffness and strength while limiting significant damage in the non-structural wall. In this study, the structural performances of such elements were evaluated using static and dynamic experimental tests. The result indicates that non-structural walls that were neither isolated by seismic slits nor anchored to the adjacent walls with longitudinal reinforcements experienced less damage and higher deformability compared with walls having seismic slits. The confinement reinforcing impact was not observed on the strength and drift capacity of the beam member, owing to the large number of transverse reinforcements. However, the confinements limited the damage and nearly prevented concrete crushing. The maximum horizontal load of the specimen could be predicted using cross-sectional analysis, and the authors propose a simple equation to predict it with sufficient accuracy.
Impact of the Reinforcement Detailing on Seismic Performance of Isolated Non-structural Walls
Authors: Safi Walid , Hibino Yo , Kusunoki Koichi , Sanada Yasushi , Mukai Tomohisa
Published: May 2020

Following the observation of severe damage to structurally isolated non-structural reinforced concrete walls after major earthquakes, researchers began to reassess the effectiveness and connection detail of non-structural walls to moment-resisting frames. A method to control damages to the non-structural wall, is to cast exterior non-structural concrete wall elements to be monolithic with frame elements, without anchoring the wall longitudinal bar. The non-anchorage of the wall longitudinal bar significantly increases the drift capacity of the wall and decreases damage. Using an experimental approach, this study assesses the influence of reinforcement detailing and quantity of the transverse reinforcements on the strength and drift capacity of the non-structural hanging wall. This study further evaluates the workability mechanism of the transverse reinforcements and reinforcement detailing with concrete. The non-anchorage of hanging walls, having boundary confinements, was found to exhibit a higher drift and strength capacity than similar walls with the anchored detailing without boundary confinements. The strength capacity of the anchored detailing hanging walls with minimum amounts of reinforcements was higher than that of the non-anchored specimen. The boundary confinements were found to be more influential on the capability of the hanging wall when placed along with non-anchored detailing reinforcement.
Colorectal Cancer and Probiotics: Are Bugs Really Drugs?
Authors: Lamichhane Purushottam , Maiolini Morgan , Alnafoosi Omar , Hussein Sedra , Alnafoosi Hasan , Umbela Stewart , Richardson Tayanna , Alla Nevien , Lamichhane Narottam , Subhadra Bobban , Deshmukh Rahul R.
Published: May 2020

Colorectal cancer (CRC) is one of the most common types of cancer worldwide. There are many factors that predispose a patient to the disease such as age, family history, ethnicity, and lifestyle. There are different genetic factors and diseases that also increase a person’s risk for developing CRC. Studies have found associations between gut microbiome and the risk for developing versus protection against CRC. Normal gut microbiome aid in daily functions of the human body such as absorption, metabolism, detoxification, and regulation of inflammation. While some species of bacteria prevent CRC development and aid in therapeutic responses to various treatment regiments, other species seem to promote CRC pathogenesis. In this regard, many studies have been conducted to not only understand the biology behind these opposing different bacterial species; but also to determine if supplementation of these tumor opposing bacterial species as probiotics lends toward decreased risk of CRC development and improved therapeutic responses in patients with CRC. In this literature review, we aim to discuss the basics on colorectal cancer (epidemiology, risk factors, targets, treatments), discuss associations between different bacterial strains and CRC, and discuss probiotics and their roles in CRC prevention and treatment.
Catalysis and Fine Chemicals
Authors: Nomura Kotohiro , Kitiyanan Boonyarach
Published: May 2020

At the event of the International Symposium on Catalysis and Fine Chemicals 2018 (C&FC2018, 10–14 December 2018, Chulalongkorn University, Bangkok, Thailand) organized by PETROMAT (co-organized by Chemical Society of Thailand and CATSJ), we decided to organize a Special Issue entitled “Catalysis and Fine Chemicals” [...]
Hydrothermal Aging of Pd/LTA Monolithic Catalyst for Complete CH4 Oxidation
Authors: Friberg Ida , Wang Aiyong , Olsson Louise
Published: May 2020

Palladium-based catalysts are known to provide high CH4 oxidation activity. One drawback for these materials is that they often lose activity in the presence of water vapor due to the formation of surface hydroxyls. It is however possible to improve the water vapor tolerance by using zeolites as support material. In this study, we have investigated Pd supported on thermally stable LTA zeolite with high framework Si/Al ratio (Si/Al = ~44) for CH4 oxidation and the effect of hydrothermal aging at temperatures up to 900 °C. High and stable CH4 oxidation activity in the presence of water vapor was observed for Pd/LTA after hydrothermal aging at temperatures ≤ 700 °C. However, aging at temperatures of 800–900 °C resulted in catalyst deactivation. This deactivation was not a result of structural collapse of the LTA zeolite as the LTA zeolite only showed minor changes in surface area, pore volume, and X-ray diffraction pattern after 900 °C aging. We suggest that the deactivation was caused by extensive formation of ion-exchanged Pd2+ together with Pd sintering. These two types of Pd species appear to have lower CH4 oxidation activity and to be more sensitive to water deactivation compared to the well dispersed Pd particles observed on the LTA support prior to the hydrothermal aging. By contrast, Pd/Al2O3 was generally sensitive to water vapor no matter of the aging temperature. Although the aging caused extensive Pd sintering in Pd/Al2O3, only minor deterioration of the CH4 oxidation activity was seen. The results herein presented show that Pd/LTA is a promising CH4 oxidation catalyst, however Pd rearrangement at high temperatures (≥800 °C) is one remaining challenge.
Hydrothermal Synthesis of rGO-TiO2 Composites as High-Performance UV Photocatalysts for Ethylparaben Degradation
Authors: Ruidíaz-Martínez Miller , Álvarez Miguel A. , López-Ramón María Victoria , Cruz-Quesada Guillermo , Rivera-Utrilla José , Sánchez-Polo Manuel
Published: May 2020

A series of reduced graphene oxide-TiO2 composites (rGO-TiO2) were prepared by hydrothermal treatment using graphite and titanium isopropoxide as raw materials. The structural, surface, electronic, and optical properties of the prepared composites were extensively characterized by N2 adsorption, FTIR, XRD, XPS, Raman spectroscopy, and DRS. GO was found to be effectively reduced and TiO2 to be in pure anatase phase in all composites obtained. Finally, experiments were performed to evaluate the effectiveness of these new materials as photocatalysts in the degradation of ethylparaben (EtP) by UV radiation. According to the band-gap energies obtained (ranging between 3.09 eV for 4% rGO-TiO2 to 2.55 eV for 30% rGO-TiO2), the rGO-TiO2 composites behave as semiconductor materials. The photocatalytic activity is highest with a rGO content of 7 wt% (7% rGO-TiO2), being higher than observed for pure TiO2 (Eg = 3.20 eV) and achieving 98.6% EtP degradation after only 40 min of treatment. However, the degradation yield decreases with higher percentages of rGO. Comparison with rGO-P25 composites showed that a better photocatalytic performance in EtP degradation is obtained with synthesized TiO2 (rGO-TiO2), probably due to the presence of the rutile phase (14.1 wt %) in commercial P25.