bluuebunny/crossref_metadata_2025 huggingface dataset:
Boronate‐Based Fluorescence Probes for the Detection of Hydrogen Peroxide
Authors: Lampard Emma V. , Sedgwick Adam C. , Sun Xiaolong , Filer Katherine L. , Hewins Samantha C. , Kim Gyoungmi , Yoon Juyoung , Bull Steven D. , James Tony D.
Published: January 2018

In this work, we synthesized a series of boronate ester fluorescence probes (E)‐4,4,5,5‐tetramethyl‐2‐(4‐styrylphenyl)‐1,3,2‐dioxaborolane (STBPin), (E)‐N,N‐dimethyl‐4‐(4‐(4,4,5,5‐tetramethyl‐1,3,2‐dioxaborolan‐2‐yl)styryl)aniline (DSTBPin), (E)‐4‐(4‐(4,4,5,5‐tetramethyl‐1,3,2‐dioxaborolan‐2‐yl)styryl)benzonitrile (CSTBPin), (E)‐2‐(4‐(4‐methoxystyryl)phenyl)‐4,4,5,5‐tetramethyl‐1,3,2‐dioxaborolane (MSTBPin), (E)‐N,N‐dimethyl‐4‐(4‐(4,4,5,5‐tetramethyl‐1,3,2‐dioxaborolan‐2‐yl)styryl)naphthalen‐1‐amine (NDSTBPin), and N,N‐dimethyl‐4‐(2‐(4‐(4,4,5,5‐tetramethyl‐1,3,2‐dioxaborolan‐2‐yl)phenyl)oxazol‐5‐yl)aniline (DAPOX‐BPin) for the detection of hydrogen peroxide (H2O2). DSTBPin and MSTBPin displayed an “Off–On” fluorescence response towards H2O2, owing to the loss of the intramolecular charge transfer (ICT) excited state. Whereas, CSTBPin displayed a decrease in fluorescence intensity in the presence of H2O2 owing to the introduction of an ICT excited state. STBPin, on the other hand, produced a small fluorescence decrease, indicating the importance of an electron‐withdrawing or electron‐donating group in these systems. Unfortunately, the longer wavelength probe, NDSTBPin, displayed a decrease in fluorescence intensity. Oxazole‐based probe DAPOX‐BPin produced a “turn‐on” response. Regrettably, DAPOX‐BPin required large concentrations of H2O2 (>3 mm) to produce noticeable changes in fluorescence intensity and, therefore, no change in fluorescence was observed in the cell imaging experiments.
Quality of life, binge eating and sexual function in participants treated for obesity with sustained release naltrexone/bupropion
Authors: Halseth A. , Shan K. , Gilder K. , Malone M. , Acevedo L. , Fujioka K.
Published: January 2018

Objective

This multicenter, randomized, controlled, open‐label trial examined weight‐related quality of life, control over eating behaviour and sexual function after 26 weeks of treatment with either 32 mg naltrexone sustained release (SR)/360 mg bupropion SR plus a comprehensive lifestyle intervention program (NB + CLI, N = 153) or usual care (UC, N = 89), which included minimal lifestyle intervention.

Methods

Impact of Weight on Quality of Life‐Lite, Binge Eating Scale and Arizona Sexual Experiences Scale were assessed at baseline (BL) and weeks 16 and 26.

Results

NB + CLI and UC participants lost 9.46 and 0.94% respectively of initial body weight at week 26 (P < 0.0001). NB + CLI participants had greater improvements in Impact of Weight on Quality of Life‐Lite total score than UC participants (P < 0.0001). In participants with moderate/severe Binge Eating Scale scores at BL, 91% of NB + CLI and 18% of UC participants experienced categorical improvements. In participants with Arizona Sexual Experiences Scale‐defined sexual dysfunction at BL, 58% of NB + CLI and 19% of UC participants no longer met dysfunction criteria at week 26. The most frequent adverse events leading to discontinuation before week 26 in NB + CLI included nausea (10.5%); anxiety (3.3%); and headache, hypertension, insomnia and palpitations (1.3% each).

Conclusion

Compared with UC, participants treated with NB + CLI experienced greater improvements in weight‐related quality of life, control over eating behaviour, and sexual function.
Latitudinal trends in Freshwater Drum population dynamics: Facing a changing climate
Authors: Abner J.K. , Phelps Q.E.
Published: January 2018

Latitudinal trends are present in multiple groups of organisms. Widely distributed species experience broad environmental gradients with differences in climate that translates to differences in dynamic rate functions. When evaluating widely distributed fishes in a large river system, one particular interest is the influence of latitude on recruitment, growth, and mortality. This study explored effects of latitude, and therefore climate, on the population dynamics of Freshwater Drum Aplodinotus grunniens along the Upper Mississippi River. Descriptive examinations of the dynamic rate functions indicated that freshwater drum populations from more northern locations experienced greater variability in recruitment, smaller maximum lengths, longer lifespans, and lower mortality rates than their southern counterparts. One potential hypothesis to explain these results relates to the temperature differences between more northern and more southern latitudes. For further explanation, individual regressions were computed between average temperature and population characteristics. These analyses suggested that temperature is a major driver of freshwater drum population attributes along this latitudinal gradient. Furthermore, we have demonstrated that a potential increased thermal regime (i.e., mid‐century and end‐century time climate change) will likely have pronounced influence on freshwater drum population characteristics. This research has implications for understanding population dynamics of widely distributed organisms in large riverine systems.
A Comparative Analysis of Attitudes on Communication Toward Stem Cell Research and Regenerative Medicine Between the Public and the Scientific Community
Authors: Shineha Ryuma , Inoue Yusuke , Ikka Tsunakuni , Kishimoto Atsuo , Yashiro Yoshimi
Published: January 2018

Owing to the rapid progress in stem cell research (SCR) and regenerative medicine (RM), society's expectation and interest in these fields are increasing. For effective communication on issues concerning SCR and RM, surveys for understanding the interests of stakeholders is essential. For this purpose, we conducted a large-scale survey with 2,160 public responses and 1,115 responses from the member of the Japanese Society for Regenerative Medicine. Results showed that the public is more interested in the post-realization aspects of RM, such as cost of care, countermeasures for risks and accidents, and clarification of responsibility and liability, than in the scientific aspects; the latter is of greater interest only to scientists. Our data indicate that an increased awareness about RM-associated social responsibility and regulatory framework is required among scientists, such as those regarding its benefits, potential accidents, abuse, and other social consequences. Awareness regarding the importance of communication and education for scientists are critical to bridge the gaps in the interests of the public and scientists.
Transparent, Flexible, Conformal Capacitive Pressure Sensors with Nanoparticles
Authors: Kim Hyeohn , Kim Gwangmook , Kim Taehoon , Lee Sangwoo , Kang Donyoung , Hwang Min‐Soo , Chae Youngcheol , Kang Shinill , Lee Hyungsuk , Park Hong‐Gyu , Shim Wooyoung
Published: January 2018

The fundamental challenge in designing transparent pressure sensors is the ideal combination of high optical transparency and high pressure sensitivity. Satisfying these competing demands is commonly achieved by a compromise between the transparency and usage of a patterned dielectric surface, which increases pressure sensitivity, but decreases transparency. Herein, a design strategy for fabricating high‐transparency and high‐sensitivity capacitive pressure sensors is proposed, which relies on the multiple states of nanoparticle dispersity resulting in enhanced surface roughness and light transmittance. We utilize two nanoparticle dispersion states on a surface: (i) homogeneous dispersion, where each nanoparticle (≈500 nm) with a size comparable to the visible light wavelength has low light scattering; and (ii) heterogeneous dispersion, where aggregated nanoparticles form a micrometer‐sized feature, increasing pressure sensitivity. This approach is experimentally verified using a nanoparticle‐dispersed polymer composite, which has high pressure sensitivity (1.0 kPa–1), and demonstrates excellent transparency (>95%). We demonstrate that the integration of nanoparticle‐dispersed capacitor elements into an array readily yields a real‐time pressure monitoring application and a fully functional touch device capable of acting as a pressure sensor‐based input device, thereby opening up new avenues to establish processing techniques that are effective on the nanoscale yet applicable to macroscopic processing.
2D Photovoltaic Devices: Progress and Prospects
Authors: Wang Lin , Huang Li , Tan Wee Chong , Feng Xuewei , Chen Li , Huang Xin , Ang Kah‐Wee
Published: January 2018

The discovery of the new class of 2D materials has stimulated extensive research interest for fundamental studies and applied technologies. Owing to their unique electronic and optical properties, which differ from their bulk counterparts and conventional optoelectronic materials, 2D materials at the atomic scale are very attractive for future photovoltaic devices. Over the past years, their great potential for photovoltaic applications has been widely investigated by creating a variety of specific device structures. Here, the recent progress made toward the exploitation of 2D materials for high‐performance photovoltaic applications is reviewed. By addressing both lateral and vertical configurations, the prospects offered by 2D materials for future generations of photovoltaic devices are elucidated. In addition, the challenges facing this rapidly progressing research field are discussed, and routes to commercially viable 2D‐material‐based photovoltaic devices are proposed.
Proactive encryption of personal area networks and small office‐home office networks under advanced encryption standard application
Authors: Moschos Achillefs , Papadimitriou Georgios , Nicopolitidis Petros
Published: January 2018

Encryption delay can be the limiting factor when transmitting data in small networks such as personal area networks (PANs) and small office‐home office networks (SOHOs). The problem arises when using predetermined encryption levels and thus the network delay is dependant on the method of encryption. In this paper, a more efficient method for conserving bandwidth in multipath networks is proposed when using advanced encryption standard (AES) as the encryption algorithm. By lowering the available choices of AES variants from 3 to 2, we can increase the encryption speed process while sacrificing a nonsignificant amount of security. In our setup, the network component that enforces the encryption of the packets decides between AES 128 and 192 and then we compare the results with the ones that use all 3 variants. This is achieved through multiple simulations runs and gathering of their results
Efficient and dynamic elliptic curve qu‐vanstone implicit certificates distribution scheme for vehicular cloud networks
Authors: Teniou Ahcene , Bensaber Boucif A.
Published: January 2018

In this paper, we introduce an efficient and dynamic elliptic curve qu‐vanstone implicit certificates distribution scheme for vehicular cloud networks. We are concerned about how to achieve efficiently and dynamically certificates distribution with a reduced cost. We design an efficient mechanism that reduces the communication cost and the computational overhead for more safety and robustness of intelligent transportation systems. Our proposal enables vehicles to request and obtain implicit certificates upon a secure request, which can be used for further signing exchanged messages. Due to the restricted nature of these certificates, a simple and efficient revocation method has been presented. It is literally based on selective revocation message delivery technique that reduces the number of messages needed for revocation phase and solves a bunch of drawbacks of existing solutions. An extensive analysis is performed to demonstrate how the proposed scheme can dynamically carry out an effective certificates distribution. We further discuss and evaluate simulation results to demonstrate the merits gained by the proposed protocol.
Evaluation of sequential Bayesian‐based method development procedures for chromatographic problems involving one, two, and three analytes
Authors: Wiczling Paweł
Published: January 2018

In this work, various sequential Bayesian‐based method development procedures associated with a search of isocratic chromatographic conditions ensuring baseline separation of one, two, and three analytes within the pre‐specified retention time window were evaluated. The accuracy and total analysis time of tested procedures were experimentally and theoretically verified and compared with the usual approaches utilizing one or two preliminary organic modifier gradients. The possession of strong and weakly informative priors about analytes was considered. The overall success rate of tested procedures was high with a small number of mistakes. It was shown that for simple problems considered here one is able to use a sequence of isocratic conditions to find the desired chromatogram. The use of one preliminary gradient was shown to be of similar accuracy and time requirements. Interestingly, the use of two preliminary gradients almost always required more time than procedures involving no or one preliminary gradient. Prior knowledge of analytes considerably shortened method development times for procedures involving no or one preliminary gradient. The sequential Bayesian‐based method development allows us to achieve a desired chromatographic separation by proper weighting of information available to the analyst at each stage of the method development process.
Adaptive distinguishing test cases of nondeterministic finite state machines: test case derivation and length estimation
Authors: El-Fakih Khaled , Yevtushenko Nina , Kushik Natalia
Published: January 2018

A top-down approach is presented for checking the existence and derivation of an adaptive distinguishing test case (called also an adaptive distinguishing sequence) for a nondeterministic finite state machine (NDFSM). When such a test case exists, the method returns a canonical test case that includes all other distinguishing tests of the given complete observable NDFSM. In the second part of the paper, a constructive approach is provided for deriving a class of complete observable NDFSMs with
n
states,
n
>  2, and 2

n

−
n
− 1 inputs such that a shortest adaptive distinguishing test case for each NDFSM in the intended class has the length (height) 2

n

−
n
− 1. In other words, we prove the reachability of the exponential upper bound on the length of a shortest adaptive distinguishing sequence for complete observable NDFSMs while for deterministic machines the upper bound is polynomial with respect to the number of states. For constructing the intended class of NDFSMs for a given
n
, we propose a special linear order over all the non-empty subsets without singletons of an
n
-element set. The obtained tight exponential upper bound initiates further research on identifying certain NDFSM classes where this upper bound is not reachable.
Spatial analyses of Ediacaran communities at Mistaken Point
Authors: Mitchell Emily G. , Butterfield Nicholas J.
Published: January 2018

Bedding-plane assemblages of Ediacaran fossils from Mistaken Point, Newfoundland, are among the oldest known records of complex multicellular life on Earth (dated to ~565 Ma). The in situ preservation of these sessile but otherwise deeply enigmatic organisms means that statistical analyses of specimen positions can be used to illuminate their underlying ecological dynamics, including the interactions between taxa.

Fossil assemblages on Mistaken Point D and E surfaces were mapped to millimeter accuracy using differentiated GPS. Spatial correlations between 10 well-defined taxa (Bradgatia, Charniid,Charniodiscus,Fractofusus, Ivesheadiomorphs, Lobate Discs,Pectinifrons,Plumeropriscum,Hiemalora, andThectardis) were identified using Bayesian network inference (BNI), and then described and analyzed using spatial point-process analysis. BNI found that the E-surface community had a complex web of interactions and associations between taxa, with all but one taxon (Thectardis) interacting with at least one other. The unique spatial distribution ofThectardissupports previous, morphology-based arguments for its fundamentally distinct nature. BNI revealed that the D-surface community showed no interspecific interactions or associations, a pattern consistent with a homogeneous environment.

On the E surface, all six of the abundant taxonomic groups (Fractofusus,Bradgatia, Charniid,Charniodiscus,Thectardis, andPlumeropriscum) were found to have a unique set of interactions with other taxa, reflecting a broad range of underlying ecological responses. Four instances of habitat associations were detected between taxa, of which two (Charniodiscus–PlumeropriscumandPlumeropriscum–Fractofusus) led to weak competition for resources. One case of preemptive competition between Charniid and Lobate Discs was detected. There were no instances of interspecific facilitation. Ivesheadiomorph interactions mirror those ofFractofususandCharniodiscus, identifying them as a form-taxonomic grouping of degradationally homogenized taphomorphs. The absence of increased fossil abundance in proximity to these taphomorphs argues against scavenging or saprophytic behaviors dominating the E-surface community.
The Valley of Juigalpa, Mayales River Subbasin Microregion (Chontales, Nicaragua) Date List I
Authors: Donner Natalia R , Geurds Alexander
Published: January 2018

The chronology of human presence in Chontales, central Nicaragua, was established by Franck Gorin (1989), who applied a combination of absolute dates and ceramic associations to build a sequence comprised of six different phases. However, interpretative and chronological issues have arisen due to two main problems. First, the sequence was based on stylistic associations to polychrome ceramics from Pacific Nicaragua. Second, the sample analyzed by Gorin shows contextual bias and lacks sufficient absolute dates. As a result, a comprehensive regional research plan was established to test the current accepted chronology, and redefine it where necessary. In this paper, we present the first date list for the Valley of Juigalpa, Mayales River subbasin (Chontales, Nicaragua). Contrary to Gorin’s proposal, which established a chronology from 500 BC through AD 1600, reports on new seventeen radiocarbon (14C) assays show a cultural sequence from 1420 ± 30 BP, or cal AD 595–660 (±2σ) through the present.
Why fast magnetic reconnection is so prevalent
Authors: Boozer Allen H.
Published: January 2018

Evolving magnetic fields are shown to generically reach a state of fast magnetic reconnection in which magnetic field line connections change and magnetic energy is released at an Alfvénic rate. This occurs even in plasmas with zero resistivity; only the finiteness of the mass of the lightest charged particle, an electron, is required. The speed and prevalence of Alfvénic or fast magnetic reconnection imply that its cause must be contained within the ideal evolution equation for magnetic fields, $\unicode[STIX]{x2202}\boldsymbol{B}/\unicode[STIX]{x2202}t=\unicode[STIX]{x1D735}\times (\boldsymbol{u}\times \boldsymbol{B})$, where $\boldsymbol{u}(\boldsymbol{x},t)$ is the velocity of the magnetic field lines. For a generic $\boldsymbol{u}(\boldsymbol{x},t)$, neighbouring magnetic field lines develop a separation that increases exponentially, as $e^{\unicode[STIX]{x1D70E}(\ell ,t)}$ with $\ell$ the distance along a line. This exponentially enhances the sensitivity of the evolution to non-ideal effects. An analogous effect, the importance of stirring to produce a large-scale flow and enhance mixing, has been recognized by cooks through many millennia, but the importance of the large-scale flow $\boldsymbol{u}$ to reconnection is customarily ignored. In part this is due to the sixty-year focus of recognition theory on two-coordinate models, which eliminate the exponential enhancement that is generic with three coordinates. A simple three-coordinate model is developed, which could be used to address many unanswered questions.
Eight new species of Pyrenulaceae from the Neotropics, with a key to 3-septate Pyrgillus species
Authors: APTROOT André , SIPMAN Harrie J. M. , MERCADO DIAZ Joel Alejandro , MENDONÇA Cléverton de Oliveira , FEUERSTEIN Shirley Cunha , CUNHA-DIAS Iane Paula Rego , PEREIRA Thamires Almeida , CÁCERES Marcela Eugenia da Silva
Published: January 2018

Eight new species of Pyrenulaceae are described as new to science from Brazil, Guyana and Puerto Rico. Pyrenula sanguineomeandrata Aptroot & Mercado Diaz (with a thallus with red, KOH+ purple pigmentation of lines or a reticulum, simple ascomata with vertical ostioles, a deep red inspersed, KOH+ orange hamathecium, and dark brown 3-septate ascospores 25–29×10–12 μm) and P. sanguineostiolata Aptroot & Mercado Diaz (with a thallus with deeply immersed simple ascomata with vertical ostioles, which are superficial and bright red, and 3-septate ascospores 25–28×9–12 μm) are described from submontane evergreen forests in Puerto Rico. Pyrenula biseptata Aptroot & M. Cáceres (with simple ascomata with vertical ostioles, an inspersed hamathecium and 2-septate ascospores 11–12×4·5–5·0 μm) and P. xanthinspersa Aptroot & M. Cáceres (with an ecorticate thallus containing lichexanthone, simple ascomata with vertical ostioles, not inspersed hamathecium and 3-septate ascospores 14–17×6·0–7·5 μm) are described from rainforest in Amazonian Brazil. Pyrenula subvariabilis Aptroot & Sipman (with fused ascomata with lateral ostioles and submuriform ascospores 17–20(–25)×6–9 μm) and Sulcopyrenula biseriata Aptroot & Sipman (with a thallus containing lichexanthone, simple ascomata with lateral ostioles and lozenge-shaped ascospores with 8 locules, (13–)15–17(–20)×8–10 (width)×6–7 (thickness) μm) are described from savannahs in Guyana. Special attention is paid to the genus Pyrgillus: two new species from the 3-septate core group of this small genus are described from Brazil, viz. P. aurantiacus Aptroot & M. Cáceres (with a corticate thallus containing lichexanthone, mazaedium with orange, KOH+ violet, UV+ red pruina and ascospores of 13–16×6·0–7·5 μm) and P. rufus Aptroot & M. Cáceres (with a corticate thallus containing lichexanthone, mazaedium with dark red, KOH+ orange, UV+ red pruina and ascospores of 15·0–17·5×5·0–6·5 μm). An updated key to the 3-septate species of Pyrgillus is provided.
Estimating the timescale of Lobaria diversification
Authors: CORNEJO Carolina , SCHEIDEGGER Christoph
Published: January 2018

Using an ITS mutation rate as calibration reference, a three-locus timetree was generated for the genus Lobaria and its most important clades. The timetree resolved most clades with strong support and gave an estimate of the diversification time for Lobaria during the early Oligocene. A fossil impression from a 12–24 million-year-old Miocene deposit is hypothesized here to belong to an ancestral Lobaria species. Additionally, the age estimate indicates that the paleoclimate and the closing or opening of the Bering Strait played a major role in shaping the current distribution of most Lobaria species. It is hypothesized that the Bering land bridge acted as a major highway during warm-temperate climate periods, but as a barrier during Arctic climate times.
Maybe it's a grime [t]ing:th-stopping among urban British youth
Authors: Drummond Rob
Published: January 2018

This article examines how voicelessth-stopping (e.g.tingforthing) is used by a group of adolescents in Manchester, UK. The data come from an ethnographic project into the speech of fourteen to sixteen year olds who have been excluded from mainstream education. Althoughth-stopping is often strongly associated with black varieties of English, multiple regression analysis finds ethnicity not to be a statistically significant factor in its production. Instead, conversational context and involvement in aspects of particular social practices—grime (rap) and dancehall music—emerge as potentially more relevant. Subsequent interactional analysis adds support to this interpretation, illustrating how the feature is being used more or less strategically (and more or less successfully) by individuals in this context in order to adopt particular stances, thereby enacting particular identities that are only tangentially related to ethnicity. I argue that use ofth-stopping in this context indexes a particular street identity that is made more available through participation in grime especially. (th-stopping, youth language, identity, ethnography, grime, hip hop)*
The Development and Psychometric Evaluation of the Group Schema Therapy Rating Scale – Revised
Authors: Bastick Emily , Bot Suili , Verhagen Simone J. W. , Zarbock Gerhard , Farrell Joan , Brand-de Wilde Odette , Arntz Arnoud , Lee Christopher William
Published: January 2018

Background: Recent research has supported the efficacy of schema therapy as a treatment for personality disorders. A group format has been developed (group schema therapy; GST), which has been suggested to improve both the clinical and cost-effectiveness of the treatment. Aims: Efficacy studies of GST need to assess treatment fidelity. The aims of the present study were to improve, describe and evaluate a fidelity measure for GST, the Group Schema Therapy Rating Scale – Revised (GSTRS-R). Method: Following a pilot study on an initial version of the scale (GSTRS), items were revised and guidelines were modified in order to improve the reliability of the scale. Students highly experienced with the scale rated recorded GST therapy sessions using the GSTRS-R in addition to a group cohesion measure, the Harvard Community Health Plan Group Cohesiveness Scale – II (GCS-II). The scores were used to assess internal consistency and inter-rater reliability. Discriminant validity was assessed by comparing the scores on the GSTRS-R with the GCS-II. Results: The GSTRS-R displayed substantial internal consistency and inter-rater reliability, and adequate discriminate validity, evidenced by a weak positive correlation with the GCS-II. Conclusions: Overall, the GSTRS-R is a reliable tool that may be useful for evaluating therapist fidelity to GST model, and assisting GST training and supervision. Initial validity was supported by a weak association with GCS-II, indicating that although associated with cohesiveness, the instrument also assesses factors specific to GST. Limitations are discussed.
Can history improve big bang health reform? Commentary
Authors: Marchildon Gregory P.
Published: January 2018

At present, the professional skills of the historian are rarely relied upon when health policies are being formulated. There are numerous reasons for this, one of which is the natural desire of decision-makers to break with the past when enactingbig bangpolicy change. This article identifies the strengths professional historians bring to bear on policy development using the establishment and subsequent reform of universal health coverage as an example. Historians provide pertinent and historically informed context; isolate the forces that have historically allowed for major reform; and separate the truly novel reforms from those attempted or implemented in the past. In addition, the historian’s use of primary sources allows potentially new and highly salient facts to guide the framing of the policy problem and its solution. This paper argues that historians are critical for constructing a viable narrative of the establishment and evolution of universal health coverage policies. The lack of this narrative makes it difficult to achieve an accurate assessment of systemic gaps in coverage and access, and the design or redesign of universal health coverage that can successfully close these gaps.
EPSPSGene Amplification Primarily Confers Glyphosate Resistance among Arkansas Palmer amaranth (Amaranthus palmeri) Populations
Authors: Singh Shilpa , Singh Vijay , Lawton-Rauh Amy , Bagavathiannan Muthukumar V. , Roma-Burgos Nilda
Published: January 2018

Research was conducted to determine whether resistance to glyphosate among Palmer amaranth (Amaranthus palmeriS. Watson) populations within the U.S. state of Arkansas was due solely to increasedEPSPSgene copy number and whether gene copy number is correlated with resistance level to glyphosate. One hundred and fifteenA. palmeriaccessions were treated with 840 g ae ha−1glyphosate. Twenty of these accessions, selected to represent a broad range of responses to glyphosate, underwent further testing. Seven of the accessions were controlled with this dose; the rest were resistant. The effective dose to cause 50% injury (ED50) for susceptible accessions ranged from 28 to 207 g ha−1. The glyphosate-resistant (GR) accessions had ED50values ranging from 494 to 1,355 g ha−1, a 3- to 48-fold resistance level compared with the susceptible standard (SS). The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene relative copy number was determined for 20 accessions, 4 plants accession−1. Resistant plants from five GR accessions (38% of resistant plants tested) did not have increasedEPSPSgene copies. Resistant plants from the remaining eight GR accessions (62% of resistant plants tested) had 19 to 224 moreEPSPSgene copies than the SS. Among the accessions tested, injury declined 4% with every additionalEPSPScopy. ED50values were directly correlated withEPSPScopy number. The highly resistant accession MIS11-B had an ED50of 1,355 g ha−1and 150 gene copies. Partial sequences ofEPSPSfrom GR accessions withoutEPSPSamplification did not contain any of the known resistance-conferring mutations. Nearly 40% of GR accessions putatively harbor non–target site resistance mechanisms. Therefore, elevatedEPSPSgene copy number is associated with glyphosate resistance amongA. palmerifrom Arkansas.
High-resolution grayscale image hidden in a laser beam
Authors: Yue Fuyong , Zhang Chunmei , Zang Xiao-Fei , Wen Dandan , Gerardot Brian D , Zhang Shuang , Chen Xianzhong
Published: January 2018

Images perceived by human eyes or recorded by cameras are usually optical patterns with spatially varying intensity or color profiles. In addition to the intensity and color, the information of an image can be encoded in a spatially varying distribution of phase or polarization state. Interestingly, such images might not be able to be directly viewed by human eyes or cameras because they may exhibit highly uniform intensity profiles. Here, we propose and experimentally demonstrate an approach to hide a high-resolution grayscale image in a square laser beam with a size of less than half a millimeter. An image with a pixel size of 300 × 300 nm is encoded into the spatially variant polarization states of the laser beam, which can be revealed after passing through a linear polarizer. This unique technology for hiding grayscale images and polarization manipulation provides new opportunities for various applications, including encryption, imaging, optical communications, quantum science and fundamental physics.
Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling
Authors: Sun Yan , Yang Dan , Xi Lei , Chen Yanlin , Fu Lixin , Sun Kexin , Yin Jiali , Li Xiaotian , Liu Shuiqing , Qin Yilu , Liu Manran , Hou Yixuan
Published: January 2018

Cancer-associated fibroblasts (CAFs) support tumorigenesis by stimulating cancer cell proliferation, and invasion, but how the premalignant stromal fibroblasts trigger epithelial changes remain unclear. We demonstrate that atypical ductal hyperplasia-associated fibroblasts (AHFs) are one kind of activated fibroblasts and stimulate cell growth and polarity change of epithelium-like tumor cell MCF-7 as CAFs-like fibroblasts. Microarray shows miR-200b and miR-200c are downregulated during AHFs and CAFs, and contribute to stromal fibroblast activity. Additionally, miR-200b/c with target gene IKKβ (inhibitor of nuclear factor kappa-B kinase β) control PAI-1 (plasminogen activator inhibitor-1) expression to regulate growth and polarity changes of MCF-7 cells through NF-κB pathway. Exploring the difference of AHFs in premalignant transformation is crucial for understanding the pathobiology of breast cancer progression.
The BAX/BAK-like protein BOK is a prognostic marker in colorectal cancer
Authors: Carberry Steven , D’Orsi Beatrice , Monsefi Naser , Salvucci Manuela , Bacon Orna , Fay Joanna , Rehm Markus , McNamara Deborah , Kay Elaine W. , Prehn Jochen H. M.
Published: January 2018

The intrinsic or mitochondrial apoptosis pathway is controlled by the interaction of antiapoptotic and pro-apoptotic members of the BCL-2 protein family. Activation of this death pathway plays a crucial role in cancer progression and chemotherapy responses. The BCL-2-related ovarian killer (BOK) possesses three BCL-2 homology domains and has been proposed to act in a similar pro-apoptotic pathway as the pro-apoptotic proteins BAX and BAK. In this study, we showed that stage II and III colorectal cancer patients possessed decreased levels of BOK protein in their tumours compared to matched normal tissue. BOK protein levels in tumours were also prognostic of clinical outcome but increased BOK protein levels surprisingly associated with earlier disease recurrence and reduced overall survival. We found no significant association of BOK protein tumour levels with ER stress markers GRP78 or GRP94 or with cleaved caspase-3. In contrast, BOK protein levels correlated with Calreticulin. These data indicate BOK as a prognostic marker in colorectal cancer and suggest that different activities of BOK may contribute to cancer progression and prognosis.
Downregulation of annexin A3 inhibits tumor metastasis and decreases drug resistance in breast cancer
Authors: Du Ruikai , Liu Bingjie , Zhou Lei , Wang Dong , He Xueyan , Xu Xiaojun , Zhang Lixing , Niu Chaoshi , Liu Suling
Published: January 2018

Annexin A3 (ANXA3) is dysregulated and plays an important role in various cancers. However, the role ofANXA3in breast cancer is still unclear. Here, we observed that the expression level ofANXA3was significantly upregulated in breast cancer tissues. ANXA3 knockdown inhibited cell invasion but promoted cell proliferation in both in vitro and in vivo assays. Furthermore, we found that ANXA3 knockdown inhibited the NFκB pathway via upregulating IκBα, resulting in mesenchymal–epithelial transition (MET) and a heterogeneity change of breast cancer stem cells (BCSCs). In addition, we demonstrated that ANXA3 knockdown increased the sensitivity of breast cancer cells to doxorubicin by increasing the drug uptake. The combination of ANXA3 knockdown and doxorubicin treatment simultaneously inhibited tumor growth and metastasis in vivo. This study described the role and mechanisms of ANXA3 in regulating BCSCs and breast cancer growth and metastasis, indicating that downregulating ANXA3 together with chemotherapy might be a novel therapeutic strategy for treating breast cancer.
Neutralization of CD95 ligand protects the liver against ischemia-reperfusion injury and prevents acute liver failure
Authors: Al-Saeedi Mohammed , Steinebrunner Niels , Kudsi Hassan , Halama Niels , Mogler Carolin , Büchler Markus W. , Krammer Peter H. , Schemmer Peter , Müller Martina
Published: January 2018

Ischemia-reperfusion injury is a common pathological process in liver surgery and transplantation, and has considerable impact on the patient outcome and survival. Death receptors are important mediators of ischemia-reperfusion injury, notably the signaling pathways of the death receptor CD95 (Apo-1/Fas) and its corresponding ligand CD95L. This study investigates, for the first time, whether the inhibition of CD95L protects the liver against ischemia-reperfusion injury. Warm ischemia was induced in the median and left liver lobes of C57BL/6 mice for 45 min. CD95Fc, a specific inhibitor of CD95L, was applied prior to ischemia. Hepatic injury was assessed via consecutive measurements of liver serum enzymes, histopathological assessment of apoptosis and necrosis and caspase assays at 3, 6, 12, 18 and 24 h after reperfusion. Serum levels of liver enzymes, as well as characteristic histopathological changes and caspase assays indicated pronounced features of apoptotic and necrotic liver damage 12 and 24 h after ischemia-reperfusion injury. Animals treated with the CD95L-blocker CD95Fc, exhibited a significant reduction in the level of serum liver enzymes and showed both decreased histopathological signs of parenchymal damage and decreased caspase activation. This study demonstrates that inhibition of CD95L with the CD95L-blocker CD95Fc, is effective in protecting mice from liver failure due to ischemia-reperfusion injury of the liver. CD95Fc could therefore emerge as a new pharmacological therapy for liver resection, transplantation surgery and acute liver failure.
Involvement of both caspase-8 and Noxa-activated pathways in endoplasmic reticulum stress-induced apoptosis in triple-negative breast tumor cells
Authors: Cano-González Ana , Mauro-Lizcano Marta , Iglesias-Serret Daniel , Gil Joan , López-Rivas Abelardo
Published: January 2018

Recent evidences indicate that triple-negative breast cancer (TNBC) cells with a mesenchymal phenotype show a basal activation of the unfolded protein response (UPR) that increases their sensitivity to endoplasmic reticulum (ER) stress although the underlying cell death mechanism remains largely unexplored. Here we show that both caspase-8-dependent and -independent apoptotic mechanisms are activated in TNBC cells undergoing sustained ER stress. Activation of the extrinsic apoptotic pathway by ER stress involves ATF4-dependent upregulation of tumor necrosis factor-related apoptosis-inducing ligand receptor 2 (TRAIL-R2/DR5). In addition, accumulation of BH3-only protein Noxa at the mitochondria further contributes to apoptosis following ER stress in TNBC cells. Accordingly, simultaneous abrogation of both extrinsic and intrinsic apoptotic pathways is required to inhibit ER stress-induced apoptosis in these cells. Importantly, persistent FLICE-inhibitory protein (FLIP) expression plays an adaptive role to prevent early activation of the extrinsic pathway of apoptosis upon ER stress. Overall, our data show that ER stress induces cell death through a pleiotropic mechanism in TNBC cells and suggest that targeting FLIP expression may be an effective approach to sensitize these tumor cells to ER stress-inducing agents.
c-FLIP is a target of the E3 ligase deltex1 in gastric cancer
Authors: Hsu Tzu-Sheng , Mo Shu-Ting , Hsu Ping-Ning , Lai Ming-Zong
Published: January 2018

The ubiquitin E3 ligase DELTEX1 (DTX1) is specifically downregulated in gastric cancer tissues, and expression of DTX1 is linked to better prognoses and survival in gastric cancer. Cellular FLICE inhibitory protein (c-FLIP) is known for its pivotal role in the resistance of cancer cells to death receptor-induced cell death. Here, we show that DTX1 is an E3 ligase for c-FLIP in gastric cancer cells. DTX1 promoted c-FLIP downregulation. Overexpression of DTX1 sensitized gastric cancer cells to TRAIL-induced apoptosis, whereas DTX1-knockdown attenuated apoptosis induction. DTX1 binds c-FLIPL and directs it into the endosome-lysosomal pathway for proteasome-independent degradation. Moreover, induction of DTX1 in AGS cells by geldanamycin conferred susceptibility of those cells to TRAIL-induced apoptosis. Our results reveal a tumor-suppressive role for DTX1 and suggest a new approach to increasing TRAIL efficacy by raising DTX1 levels in gastric cancer therapy. DTX1 also enhanced c-FLIP degradation and FasL-induced and TRAIL-induced apoptosis in T cells, suggesting that DTX1 constitutes one of the physiological mechanisms regulating c-FLIP stability.
SIRT1 induces epithelial-mesenchymal transition by promoting autophagic degradation of E-cadherin in melanoma cells
Authors: Sun Ting , Jiao Lin , Wang Yangxia , Yu Yan , Ming Liang
Published: January 2018

Melanoma is highly metastatic, and understanding of its molecular mechanism is urgently needed for the development of therapeutic targets and prognostic assessment for metastatic melanoma. SIRT1 is a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase, belonging to the mammalian sirtuin family. It has been reported that SIRT1 is associated with metastasis in various cancers. However, the molecular mechanism of SIRT1 in melanoma metastasis remains to be clarified. Here we report that SIRT1 induces the epithelial–mesenchymal transition (EMT) by accelerating E-cadherin degradation via autophagy and facilitates melanoma metastasis. Initially, we found that SIRT1 expression was frequently elevated in metastatic melanoma compared with primary melanoma. In addition, SIRT1 induced the EMT and promoted cell migration and invasion by decreasing E-cadherin expression. Further work demonstrated that SIRT1 accelerated the autophagic degradation of E-cadherin through deacetylation of Beclin 1. In addition, inhibition of autophagy recovered E-cadherin expression and suppressed cell migration and invasion by delaying the degradation of E-cadherin in SIRT1-overexpressing cells. Overall, our findings reveal a novel molecular mechanism for SIRT1 in melanoma metastasis, indicating that SIRT1 may serve as a viable therapeutic target for metastatic melanoma.
IRE1α deficiency promotes tumor cell death and eIF2α degradation through PERK dipendent autophagy
Authors: Storniolo Antonello , Alfano Vincenzo , Carbotta Sabino , Ferretti Elisabetta , Di Renzo Livia
Published: January 2018

Sensors of endoplasmic reticulum (ER) stress function in a co-ordinated manner. In the present study we investigated the relationship between IRE1α and PERK pathways and survival of ER stressed U937 cells and BC3 cells. To this end, we investigated the effects of a subcytotoxic concentration of Tunicamycin in IRE1α-proficient and in IRE1α-deficient cells, by pharmacological inhibition with 4μ8 C or down-regulation by specific siRNA. We show that either type of IRE1α deficiency affects eIF2α expression and causes cell death increase. GSK2606414, a PERK inhibitor, and PERK specific siRNA prevent eIF2α down-regulation and restore cell survival. Degradation of this protein is due to autophagy, as it is prevented by bafilomycin and not by proteasome inhibition. Furthermore, activation of the autophagy flux is PERK dependent. Also the Cathepsin B inhibitor CA074 prevents eIF2α from degradation and reduces cell death. Altogether, these results show that IRE1α deficiency in ER stressed cells leads to an unexpected decrease of eIF2α, an important molecule for protein translation, through PERK dependent autophagy. Thus, IRE1/XBP1 inhibitors may represent a feasible strategy for tumor therapy, while PERK inhibitors may vanish the goal.
Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis
Authors: Matthes Frank , Hettich Moritz M. , Schilling Judith , Flores-Dominguez Diana , Blank Nelli , Wiglenda Thomas , Buntru Alexander , Wolf Hanna , Weber Stephanie , Vorberg Ina , Dagane Alina , Dittmar Gunnar , Wanker Erich , Ehninger Dan , Krauss Sybille
Published: January 2018

Alzheimer’s disease (AD) is characterized by two neuropathological hallmarks: senile plaques, which are composed of amyloid-β (Aβ) peptides, and neurofibrillary tangles, which are composed of hyperphosphorylated tau protein. Aβ peptides are derived from sequential proteolytic cleavage of the amyloid precursor protein (APP). In this study, we identified a so far unknown mode of regulation of APP protein synthesis involving the MID1 protein complex: MID1 binds to and regulates the translation of APP mRNA. The underlying mode of action of MID1 involves the mTOR pathway. Thus, inhibition of the MID1 complex reduces the APP protein level in cultures of primary neurons. Based on this, we used one compound that we discovered previously to interfere with the MID1 complex, metformin, for in vivo experiments. Indeed, long-term treatment with metformin decreased APP protein expression levels and consequently Aβ in an AD mouse model. Importantly, we have initiated the metformin treatment late in life, at a time-point where mice were in an already progressed state of the disease, and could observe an improved behavioral phenotype. These findings together with our previous observation, showing that inhibition of the MID1 complex by metformin also decreases tau phosphorylation, make the MID1 complex a particularly interesting drug target for treating AD.
A cerebellar mechanism for learning prior distributions of time intervals
Authors: Narain Devika , Remington Evan D. , Zeeuw Chris I. De , Jazayeri Mehrdad
Published: January 2018

Knowledge about the statistical regularities of the world is essential for cognitive and sensorimotor function. In the domain of timing, prior statistics are crucial for optimal prediction, adaptation and planning. Where and how the nervous system encodes temporal statistics is, however, not known. Based on physiological and anatomical evidence for cerebellar learning, we develop a computational model that demonstrates how the cerebellum could learn prior distributions of time intervals and support Bayesian temporal estimation. The model shows that salient features observed in human Bayesian time interval estimates can be readily captured by learning in the cerebellar cortex and circuit level computations in the cerebellar deep nuclei. We test human behavior in two cerebellar timing tasks and find prior-dependent biases in timing that are consistent with the predictions of the cerebellar model.
Early Cambrian fuxianhuiids from China reveal origin of the gnathobasic protopodite in euarthropods
Authors: Yang Jie , Ortega-Hernández Javier , Legg David A. , Lan Tian , Hou Jin-bo , Zhang Xi-guang
Published: January 2018

Euarthropods owe their evolutionary and ecological success to the morphological plasticity of their appendages. Although this variability is partly expressed in the specialization of the protopodite for a feeding function in the post-deutocerebral limbs, the origin of the former structure among Cambrian representatives remains uncertain. Here, we describeAlacaris mirabilisgen. et sp. nov. from the early Cambrian Xiaoshiba Lagerstätte in China, which reveals the proximal organization of fuxianhuiid appendages in exceptional detail. Proximally, the post-deutocerebral limbs possess an antero-posteriorly compressed protopodite with robust spines. The protopodite is attached to an endopod with more than a dozen podomeres, and an oval flap-shaped exopod. The gnathal edges of the protopodites form an axial food groove along the ventral side of the body, indicating a predatory/scavenging autecology. A cladistic analysis indicates that the fuxianhuiid protopodite represents the phylogenetically earliest occurrence of substantial proximal differentiation within stem-group Euarthropoda illuminating the origin of gnathobasic feeding.
Predicting phase behavior of grain boundaries with evolutionary search and machine learning
Authors: Zhu Qiang , Samanta Amit , Li Bingxi , Rudd Robert E. , Frolov Timofey
Published: January 2018

The study of grain boundary phase transitions is an emerging field until recently dominated by experiments. The major bottleneck in the exploration of this phenomenon with atomistic modeling has been the lack of a robust computational tool that can predict interface structure. Here we develop a computational tool based on evolutionary algorithms that performs efficient grand-canonical grain boundary structure search and we design a clustering analysis that automatically identifies different grain boundary phases. Its application to a model system of symmetric tilt boundaries in Cu uncovers an unexpected rich polymorphism in the grain boundary structures. We find new ground and metastable states by exploring structures with different atomic densities. Our results demonstrate that the grain boundaries within the entire misorientation range have multiple phases and exhibit structural transitions, suggesting that phase behavior of interfaces is likely a general phenomenon.