bluuebunny/crossref_metadata_2025 huggingface dataset:
The “Lonely Game”: Baseball, Kierkegaard, and the Spiritual Life
Authors: Barnett Christopher B.
Published: April 2020

This essay aims to show that baseball's time-honored emphases on physical and spiritual discipline follow from its metaphysical imaginary. In turn, it will reason that Christian life and thought are capable of illuminating baseball—and vice versa. The argument will proceed as follows: First, both Christianity and baseball frame their worlds in terms of emanation (exitus) and return (reditus): “players” leave home and aim to return home; second, though players belong to a team or community (ecclesia), the task of returning home is ultimately a solitary one; it has to be done by the individual player, even if the team, too, benefits from the individual's undertaking; and third, the spiritual or attitudinal development of the individual is thus crucial: players have to attend to how they approach the “game,” particularly in terms of their internal comportment. This last point will receive special attention: it will be reasoned that Søren Kierkegaard's spiritual writings, tendered for the existential “upbuilding” (Opbyggelse) of “the single individual” (den Enkelte), might likewise offer upbuilding insights for the individuals who play baseball—a sport that John Updike once called “an essentially lonely game.”
Forecasting the cumulative number of COVID-19 deaths in China: a Boltzmann function-based modeling study
Authors: Gao Yuanyuan , Zhang Zuqin , Yao Wei , Ying Qi , Long Cheng , Fu Xinmiao
Published: April 2020

The COVID-19 outbreak is ongoing in China. Here, Boltzmann function-based analyses reveal the potential total numbers of COVID-19 deaths: 3,260 (95% confidence interval [CI], 3187–3394) in China; 110 (95% CI, 109–112) in Hubei Province; 3,174 (95% CI, 3095–3270) outside Hubei; 2,550 (95% CI, 2494–2621) in Wuhan City; and 617 (95% CI, 607–632) outside Wuhan.
Resveratrol reduces oxidative damage and inflammation in mice infected withTrichinella spiralis
Authors: Elgendy D.I. , Othman A.A. , Hasby Saad M.A. , Soliman N.A. , Mwafy S.E.
Published: April 2020

Trichinellosis is a serious food-borne zoonotic infection of cosmopolitan distribution. Currently, treatment for trichinellosis is far from ideal. Given the important role of oxidative stress and immune-mediated inflammation in the pathogenesis of trichinellosis, this study was designed to evaluate the possible protective effects of resveratrol (RSV) during the intestinal and muscular phases ofTrichinella spiralisinfection in mice. The oral administration of RSV at a dose of 20 mg/kg once daily for two weeks resulted in significant reductions in both adult and larval counts; significant improvements in the redox status of the small intestine and muscles; a significant reduction in interleukin 4, pentraxin 3 and vascular endothelial growth factor expression; and the mitigation of intestinal and muscular inflammation. In conclusion, this study identifies RSV as a promising agent for the treatment of experimental trichinellosis, and more studies in experimental animals and humans are worth consideration.
Cost of interspecific competition between trematode colonies
Authors: Mouritsen K.N. , Elkjær C.K.
Published: April 2020

In a range of trematode species, specific members of the parthenitae colony infecting the molluscan first intermediate host appear specialized for defence against co-infecting species. The evolution of such division of labour requires that co-infection entails fitness costs. Yet, this premise has very rarely been tested in species showing division of labour. Using Himasthla elongata (Himasthlidae) and Renicola roscovita (Renicolidae) infecting periwinkles Littorina littorea as study system, we show that the size of emerged cercariae is markedly reduced in both parasite species when competing over host resources. Cercarial longevity, on the other hand, is negatively influenced by competition only in R. roscovita. Season, which may impact the nutritional state of the host, also affects cercarial size, but only in H. elongata. Hence, our study underlines that cercarial quality is, indeed, compromised by competition, not only in the inferior R. roscovita (no division of labour) but also in the competitively superior H. elongata (division of labour).
Contact endoscopy for detection of residual or recurrent disease after radiotherapy for squamous cell carcinoma of the upper aerodigestive tract
Authors: Kumar S , Mishra A K , Galagali J R , Sethi A , Malik A
Published: April 2020

Objective

The aim of this study was to evaluate contact endoscopy in detecting local treatment failures post-radiotherapy in squamous cell carcinoma of the upper aerodigestive tract.

Method

A total of 135 consecutive patients with suspected residual or recurrent cancer after definitive radiotherapy underwent contact endoscopy before biopsy. Contact endoscopy findings were compared with histopathological examination findings. Contact endoscopy could not be completed in 7 patients (5.9 per cent) and histopathological examination was inconclusive in 5 patients (3.7 per cent). The findings of the remaining 123 patients were compared.

Results

The sensitivity, specificity and accuracy of contact endoscopy were 88.75, 88.72 and 86.99 per cent, respectively, with similar results across various sites of upper aerodigestive tract. Inter-observer kappa value was 0.86 (95 per cent confidence interval: 0.79–0.93). The intra-observer kappa value was 0.93 (95 per cent confidence interval: 0.87–1.00) for the first observer and 0.95 (95 per cent confidence interval: 0.90–1.00) for second and third observers.

Conclusion

Contact endoscopy showed the same high sensitivity and specificity with low inter- and intra-observer variability in detecting post-radiotherapy failures in cancer of the upper aerodigestive tract as has been shown in non-irradiated tissues in earlier studies.
Remembering the Fall of the Habsburg Monarchy One Hundred Years on: Three Master Interpretations
Authors: Evans R. J. W.
Published: April 2020

This article was first conceived as a commemorative address for the centenary of the extinction of the Habsburg monarchy, which occurred in November 1918. It seeks to take a correspondingly broad view, geographically and chronologically, of the factors that occasioned that collapse. It addresses three main themes, structured loosely around three classic historiographical analyses of the monarchy as a whole. The great irony of the last phase of Habsburg rule in Central Europe is that it was undermined by precisely those elements in the politics and society of the region that seemed, on the face of things, to derive most advantage from it. The article concentrates on the long-term dysfunctionality caused by the evolution of the Hungarian and German problems, and by the progressive enfeeblement of dynastic institutions. It also engages more briefly with a countervailing phenomenon, that some of those interests most conspicuously spurned by central government might have been the readiest to rescue it. On the argument presented here, World War I, which finally brought the monarchy low, was a catalyst rather than an independent determiner of that outcome.
Intertwined Itineraries: Debt, Decolonization, and International Law in Post-World War II South Asia
Authors: Ramnath Kalyani
Published: April 2020

This Article brings a Tamil-speaking Chettiar widow and a Dutch scholar of international law - two seemingly disparate characters - together through a footnote. Set against the background of decolonizing South and Southeast Asia in the aftermath of World War Two, it follows the judgment in a little-known suit for recovery of debt, filed at a district-level civil court in Madras in British India, which escaped the attention of local legal practitioners, but made its way into an international law treatise compiled and written in Utrecht, twenty years later. Instead of using it to trace how South Asian judiciaries interpreted international law, the Article looks at why claims to international law were made by ordinary litigants like Chettiar women in everyday cases like debt settlements, and how they became “evidence” of state practice for international law. These intertwined itineraries of law, that take place against the Japanese occupation of Burma and the Dutch East Indies and the postwar reconstruction efforts in Rangoon, Madras and Batavia, show how jurisdictional claims made by ordinary litigants form an underappreciated archive for histories of international law. In talking about the creation and circulation of legal knowledges, this Article argues that this involves thinking about and writing from footnotes, postscripts and marginalia - and the lives that are intertwined in them.
Impact of improved observed hand hygiene on bloodstream infection rates in Ireland. A prospective segmented regression analysis, 2009–2016
Authors: Smiddy M.P. , Murphy O.M. , Savage E. , Fitzgerald A.P. , FitzGerald S. , Browne J.
Published: April 2020

Participation in European surveillance for bloodstream infection (BSI) commenced in Ireland in 1999 with all laboratories (n = 39) participating by 2014. Observational hand hygiene auditing (OHHA) was implemented in 2011. The aim of this study was to evaluate the impact of OHHA on hand hygiene compliance, alcohol hand rub (AHR) procurement and the incidence of sensitive and resistant Staphylococcus aureus and Enterococcus faecium and faecalis BSI. A prospective segmented regression analysis was performed to determine the temporal association between OHHA and outcomes. Observed hand hygiene improved from 74.7% (73.7–75.6) in 2011 to 90.8% (90.1–91.3) in 2016. AHR procurement increased from 20.1 l/1000 bed days used (BDU) in 2009 to 33.2 l/1000 BDU in 2016. A pre-intervention reduction of 2% per quarter in the ratio of methicillin sensitive Staphylococcus aureus BSI/BDU stabilized in the time period after the intervention (P < 0.01). The ratio of Methicillin resistant Staphylococcus aureus (MRSA) BSI/BDU was decreasing by 5% per quarter pre-intervention, this slowed to 2% per quarter post intervention, (P < 0.01). There was no significant change in the ratio of vancomycin sensitive (P = 0.49) or vancomycin resistant (P = 0.90) Enterococcus sp. BSI/BDU post intervention. This study shows national OHHA increased observed hand hygiene compliance and AHR procurement, however there was no associated reduction in BSI.
Increased neutrophil percentage-to-albumin ratio is associated with all-cause mortality in patients with severe sepsis or septic shock
Authors: Gong Yuqiang , Li Diwen , Cheng Bihuan , Ying Binyu , Wang Benji
Published: April 2020

There has been no study exploring the prognostic values of neutrophil percentage-to-albumin ratio (NPAR). We hypothesised that NPAR is a novel marker of inflammation and is associated with all-cause mortality in patients with severe sepsis or septic shock. Patient data were extracted from the MIMIC-III V1.4 database. Only the data for the first intensive care unit (ICU) admission of each patient were used and baseline data were extracted within 24 h after ICU admission. The clinical endpoints were 30-, 90- and 365-day all-cause mortality in critically ill patients with severe sepsis or septic shock. Cox proportional hazards models and subgroup analyses were used to determine the relationship between NPAR and these clinical endpoints. A total of 2166 patients were eligible for this analysis. In multivariate analysis, after adjustments for age, ethnicity and gender, higher NPAR was associated with increased risk of 30-, 90- and 365-day all-cause mortality in critically ill patients with severe sepsis or septic shock. Furthermore, after adjusting for more confounding factors, higher NPAR remained a significant predictor of all-cause mortality (tertile 3 vs. tertile 1: HR, 95% CI: 1.29, 1.04–1.61; 1.41, 1.16–1.72; 1.44, 1.21–1.71). A similar trend was observed in NPAR levels stratified by quartiles. Higher NPAR was associated with increased risk of all-cause mortality in critically ill patients with severe sepsis or septic shock.
Hungary’s Over-powerful Government Party and the Desperate Opposition
Authors: Susánszky Pál , Unger Anna , Kopper Ákos
Published: April 2020

This paper focuses on Hungary’s illiberal regimes from the perspective of the opposition – a perspective we believe has been under-scrutinized so far. It argues that in order to understand the regime’s success it is crucial to explain why opposition parties have been unable to offer a credible alternative. The paper argues that the opposition is in a trap with three unfavourable factors mutually strengthening each other and undermining its ability for success. They are (1) the cartel party system; (2) the weak social embeddedness of opposition parties; and (3) lack of their presence in local politics. At the same time, we also point out that the rise of a tiny joke-party’s success (The Hungarian Two-Tailed Dog Party) highlights that these obstacles can be overcome even with very limited resources. Nevertheless, it is still unclear if the Dog Party’s innovative approach to doing politics could be ‘institutionalized’ – i.e. if it offers a feasible model strategy for opposition parties – or it is only an efficient way of mocking and criticizing how the political system operates.
Parallelism and Antithesis: Structural Principles in the Mind and in Literature from a Chinese Perspective
Authors: Longxi Zhang
Published: April 2020

Roman Jakobson famously defined poetry as pivoting on the metaphorical axis with parallelism as a major feature, and James Kugel argues that parallelism is the defining feature of biblical poetry. The parallel structure – including its variations of symmetry and antithesis – is crucial for classical Chinese poetry. In drawing on both Chinese and Western critical views on the symmetrical structure of parallelism and antithesis, this paper will explore the relationship between the cognitive and linguistic correlation in the formation of parallel structure in literary language, particularly poetry, and argue for the basis of parallelism as deeply embedded in the mind and manifested in literary expressions.
From Classical Compositions to Contemporary Seriality
Authors: Janhsen Angeli
Published: April 2020

Classical compositions, such as those of Claude Lorrain (1600–1680), show balanced situations where the depicted objects seem to represent beauty – they appear to exist in an ideal order; they seem right, once and for all. They allow their viewers, who live in a contingent world, believe that unity and beauty are attainable. English landscape gardens in the eighteenth century offered viewers the experience of moving in real settings that seem to reproduce the canvasses of classical landscape painting. Contemporary visitors know this is an imitation; they appreciate that this is not everyday reality but a kind of realized utopia. Artworks such as the early ones by Rembrandt Harmenszoon van Rijn (1606–1669) and those from the nineteenth century onwards (Paul Cézanne, Claude Monet), make us understand that a final rightness is not possible; that it is only possible to try again and again to attain the real – without ever arriving at the one right solution. In their endeavour to depict reality, these artists engage in a serial elaboration of the same subjects and objects of their art. These series of nearly the same subjects invoke the impossibility of getting it right once and for all. Piet Mondrian illustrates the development from seriality, as repeated attempts to depict the same with a difference, to the practice of seriality as an experiment in symmetry and balance that both goes back to Claude Lorrain and modernizes the concept of the ideal for application to abstract art. In contemporary art after the Second World War serial structures in single artworks were common, thus superseding compositions with their discredited promise of ideality. Series occurred not only in the contemporary visual arts, but also in literature, music and in the theatre. The repetitive structures of Minimal Art offer to the beholder the possibility of experiencing ‘reality’ in an age of media and fakes. My example will be the repetitive structures of Christian Boltanskiʼs oeuvre which provides an opportunity to reflect on the similarity and difference of people in contemporary democracies. My main argument is therefore that the change from composition to seriality corresponds to historical change.
Social Constructions of Rape at Columbia University and Barnard College, 1955–90
Authors: Abu-Odeh Desiree , Khan Shamus , Nathanson Constance A.
Published: April 2020

Sex on college campuses has fascinated scholars, reporters, and the public since the advent of coeducational higher education in the middle of the nineteenth century. But the emergence of rape on campus as a public problem is relatively recent. This article reveals the changing social constructions of campus rape as a public problem through a detailed examination of newspaper reporting on this issue as it unfolded at Columbia University and Barnard College between 1955 and 1990. Adapting Joseph R. Gusfield’s classic formulation of public problem construction, we show the ways police and other judicial and law enforcement authorities, feminists, university faculty, student groups, university administrators, and health professionals and institutions have struggled over ownership of how the problem should be defined and described, attribution of responsibility for addressing the problem, and prescriptions for what is to be done. Our findings show how beginning in the late 1960s and early 1970s, the simultaneous swelling of the women’s liberation movement and the exponential integration of women into previously male-dominated institutions of higher education and medicine catalyzed the creation of new kinds of knowledge, institutions, and expertise to address rape and sexual violence more broadly on college campuses. New actors—feminists and health professionals—layered frames of gender and health over those of crime and punishment to fundamentally transform how we understand rape on campus, and beyond.
Determining the critical period for broadleaf weed control in high-yielding cotton using mungbean as a mimic weed
Authors: Charles Graham W. , Sindel Brian M. , Cowie Annette L. , Knox Oliver G. G.
Published: April 2020

Research using the critical period for weed control (CPWC) has shown that high-yielding cotton crops are very sensitive to competition from grasses and large broadleaf weeds, but the CPWC has not been defined for smaller broadleaf weeds in Australian cotton. Field studies were conducted over five seasons from 2003 to 2015 to determine the CPWC for smaller broadleaf weeds, using mungbean as a mimic weed. Mungbean was planted at densities of 1, 3, 6, 15, 30, and 60 plants m−2 with or after cotton emergence and added and removed at approximately 0, 150, 300, 450, 600, 750, and 900 degree days of crop growth (GDD). Mungbean competed strongly with cotton, with season-long interference; 60 mungbean plants m−2 resulted in an 84% reduction in cotton yield. A dynamic CPWC function was developed for densities of 1 to 60 mungbean plants m−2 using extended Gompertz and exponential curves including weed density as a covariate. Using a 1% yield-loss threshold, the CPWC defined by these curves extended for the full growing season of the crop at all weed densities. The minimum yield loss from a single weed control input was 35% at the highest weed density of 60 mungbean plants m−2. The relationship for the critical time of weed removal was further improved by substituting weed biomass for weed density in the relationship.
Grandin Receives 2019 John Wahr Early Career Award
Authors:   AGU
Published: April 2020

Rapha�l Grandin received the 2019 John Wahr Early Career Award at AGU’s Fall Meeting 2019, held 9–13 December in San Francisco, Calif. The award is given “in recognition of major advances in geodesy.”
Dystrophic calcification and heterotopic ossification in fibrocartilaginous tissues of the spine in diffuse idiopathic skeletal hyperostosis (DISH)
Authors: Fournier Dale E. , Kiser Patti K. , Beach Ryan J. , Dixon S. Jeffrey , Séguin Cheryle A.
Published: April 2020

Diffuse idiopathic skeletal hyperostosis (DISH) is a prevalent noninflammatory spondyloarthropathy characterized by ectopic mineral formation along the anterolateral aspect of the vertebral column, yet little is known about its underlying pathogenesis. Our objective was to evaluate the histopathological features and composition of ectopic mineral within spinal tissues affected by DISH in humans. Thoracic spine segments from six embalmed cadaveric donors (one female and five males; median age 82 years) meeting the radiographic diagnostic criteria for DISH were evaluated using radiological, histological, and physical analyses. Overall, the histological features of ectopic mineralization at individual motion segments were heterogeneous, including regions of heterotopic ossification and dystrophic calcification. Heterotopic ossifications were characterized by woven and lamellar bone, multifocal areas of metaplastic cartilage, and bony bridges along the anterior aspect of the intervertebral disc space. Dystrophic calcifications were characterized by an amorphous appearance, a high content of calcium and phosphorus, an X-ray diffraction pattern matching that of hydroxyapatite, and radiodensities exceeding that of cortical bone. Dystrophic calcifications were found within the anterior longitudinal ligament and annulus fibrosus in motion segments both meeting and not meeting the radiographic criteria for DISH. In summary, our findings indicate that in DISH, ectopic mineral forms along the anterior aspect of the spine by both heterotopic ossification and dystrophic calcification of fibrocartilaginous tissues. Although both types of ectopic mineralization are captured by current radiographic criteria for DISH, dystrophic calcification may reflect a distinct disease process or an early stage in the pathogenesis of DISH.
The regulatory network among CircHIPK3, LncGAS5, and miR-495 promotes Th2 differentiation in allergic rhinitis
Authors: Zhu Xiaoyuan , Wang Xueping , Wang Ying , Zhao Yulin
Published: April 2020

Allergic rhinitis (AR) is a common allergic disease which is characterized by the promotion of Th2 differentiation of CD4+ T cells. However, the mechanisms underlying Th2 differentiation remain unclear. Non-coding RNAs play a critical role in Th2 differentiation, whereas few studies have revealed the interactions among long non-coding RNAs, circular RNAs, and microRNAs. In this study, the differential expressions of several circRNAs and lncRNAs were compared in nasal mucosa samples of AR patients and mice with experimentally induced AR as compared to healthy controls. The results showed that the highly expressed CircHIPK3 and LncGAS5 promoted Th2 differentiation of ovalbumin-induced CD4+ T cells and aggravated nasal symptoms of AR mice. We also found that CircHIPK3 and LncGAS5 induced the upregulation of Th2 cell-specific transcript factor GATA-3 via modulating their common target miR-495. Meanwhile, the intranasal administration of CircHIPK3 or LncGAS5 knockdown lentivirus decreased nasal symptoms of AR mice. In conclusion, our findings indicated that the interactions among CircHIPK3, LncGAS5, and miR-495 play a critical role in the regulation of Th2 differentiation in AR.
Magnetic field induced quantum phases in a tensor network study of Kitaev magnets
Authors: Lee Hyun-Yong , Kaneko Ryui , Chern Li Ern , Okubo Tsuyoshi , Yamaji Youhei , Kawashima Naoki , Kim Yong Baek
Published: April 2020

Recent discovery of the half quantized thermal Hall conductivity in $$\alpha$$
α
-RuCl$${}_{3}$$

3

, a candidate material for the Kitaev spin liquid, suggests the presence of a highly entangled quantum state in external magnetic fields. This field induced phase appears between the low field zig-zag magnetic order and the high field polarized state. Motivated by this experiment, we study possible field induced quantum phases in theoretical models of the Kitaev magnets, using the two dimensional tensor network approach or infinite tensor product states. We find various quantum ground states in addition to the chiral Kitaev spin liquid occupying a small area in the phase diagram. They form a band of emergent quantum phases in an intermediate window of external magnetic fields, somewhat reminiscent of the experiment. We discuss the implications of these results in view of the experiment and previous theoretical studies.
Dual functions of Aire CARD multimerization in the transcriptional regulation of T cell tolerance
Authors: Huoh Yu-San , Wu Bin , Park Sehoon , Yang Darren , Bansal Kushagra , Greenwald Emily , Wong Wesley P. , Mathis Diane , Hur Sun
Published: April 2020

Aggregate-like biomolecular assemblies are emerging as new conformational states with functionality. Aire, a transcription factor essential for central T cell tolerance, forms large aggregate-like assemblies visualized as nuclear foci. Here we demonstrate that Aire utilizes its caspase activation recruitment domain (CARD) to form filamentous homo-multimers in vitro, and this assembly mediates foci formation and transcriptional activity. However, CARD-mediated multimerization also makes Aire susceptible to interaction with promyelocytic leukemia protein (PML) bodies, sites of many nuclear processes including protein quality control of nuclear aggregates. Several loss-of-function Aire mutants, including those causing autoimmune polyendocrine syndrome type-1, form foci with increased PML body association. Directing Aire to PML bodies impairs the transcriptional activity of Aire, while dispersing PML bodies with a viral antagonist restores this activity. Our study thus reveals a new regulatory role of PML bodies in Aire function, and highlights the interplay between nuclear aggregate-like assemblies and PML-mediated protein quality control.
Ancestry deconvolution and partial polygenic score can improve susceptibility predictions in recently admixed individuals
Authors: Marnetto Davide , Pärna Katri , Läll Kristi , Molinaro Ludovica , Montinaro Francesco , Haller Toomas , Metspalu Mait , Mägi Reedik , Fischer Krista , Pagani Luca
Published: April 2020

Polygenic Scores (PSs) describe the genetic component of an individual’s quantitative phenotype or their susceptibility to diseases with a genetic basis. Currently, PSs rely on population-dependent contributions of many associated alleles, with limited applicability to understudied populations and recently admixed individuals. Here we introduce a combination of local ancestry deconvolution and partial PS computation to account for the population-specific nature of the association signals in individuals with admixed ancestry. We demonstrate partial PS to be a proxy for the total PS and that a portion of the genome is enough to improve susceptibility predictions for the traits we test. By combining partial PSs from different populations, we are able to improve trait predictability in admixed individuals with some European ancestry. These results may extend the applicability of PSs to subjects with a complex history of admixture, where current methods cannot be applied.
A salvage pathway maintains highly functional respiratory complex I
Authors: Szczepanowska Karolina , Senft Katharina , Heidler Juliana , Herholz Marija , Kukat Alexandra , Höhne Michaela Nicole , Hofsetz Eduard , Becker Christina , Kaspar Sophie , Giese Heiko , Zwicker Klaus , Guerrero-Castillo Sergio , Baumann Linda , Kauppila Johanna , Rumyantseva Anastasia , Müller Stefan , Frese Christian K. , Brandt Ulrich , Riemer Jan , Wittig Ilka , Trifunovic Aleksandra
Published: April 2020

Regulation of the turnover of complex I (CI), the largest mitochondrial respiratory chain complex, remains enigmatic despite huge advancement in understanding its structure and the assembly. Here, we report that the NADH-oxidizing N-module of CI is turned over at a higher rate and largely independently of the rest of the complex by mitochondrial matrix protease ClpXP, which selectively removes and degrades damaged subunits. The observed mechanism seems to be a safeguard against the accumulation of dysfunctional CI arising from the inactivation of the N-module subunits due to attrition caused by its constant activity under physiological conditions. This CI salvage pathway maintains highly functional CI through a favorable mechanism that demands much lower energetic cost than de novo synthesis and reassembly of the entire CI. Our results also identify ClpXP activity as an unforeseen target for therapeutic interventions in the large group of mitochondrial diseases characterized by the CI instability.
Antibacterial effects of nanopillar surfaces are mediated by cell impedance, penetration and induction of oxidative stress
Authors: Jenkins J. , Mantell J. , Neal C. , Gholinia A. , Verkade P. , Nobbs A. H. , Su B.
Published: April 2020

Some insects, such as dragonflies, have evolved nanoprotrusions on their wings that rupture bacteria on contact. This has inspired the design of antibacterial implant surfaces with insect-wing mimetic nanopillars made of synthetic materials. Here, we characterise the physiological and morphological effects of mimetic titanium nanopillars on bacteria. The nanopillars induce deformation and penetration of the Gram-positive and Gram-negative bacterial cell envelope, but do not rupture or lyse bacteria. They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of oxidative stress proteins. Our results indicate that nanopillars’ antibacterial activities may be mediated by oxidative stress, and do not necessarily require bacterial lysis.
High efficiency and stability of ink-jet printed quantum dot light emitting diodes
Authors: Xiang Chaoyu , Wu Longjia , Lu Zizhe , Li Menglin , Wen Yanwei , Yang Yixing , Liu Wenyong , Zhang Ting , Cao Weiran , Tsang Sai-Wing , Shan Bin , Yan Xiaolin , Qian Lei
Published: April 2020

The low efficiency and fast degradation of devices from ink-jet printing process hinders the application of quantum dot light emitting diodes on next generation displays. Passivating the trap states caused by both anion and cation under-coordinated sites on the quantum dot surface with proper ligands for ink-jet printing processing reminds a problem. Here we show, by adapting the idea of dual ionic passivation of quantum dots, ink-jet printed quantum dot light emitting diodes with an external quantum efficiency over 16% and half lifetime of more than 1,721,000 hours were reported for the first time. The liquid phase exchange of ligands fulfills the requirements of ink-jet printing processing for possible mass production. And the performance from ink-jet printed quantum dot light emitting diodes truly opens the gate of quantum dot light emitting diode application for industry.
Prompt rewetting of drained peatlands reduces climate warming despite methane emissions
Authors: Günther Anke , Barthelmes Alexandra , Huth Vytas , Joosten Hans , Jurasinski Gerald , Koebsch Franziska , Couwenberg John
Published: April 2020

Peatlands are strategic areas for climate change mitigation because of their matchless carbon stocks. Drained peatlands release this carbon to the atmosphere as carbon dioxide (CO2). Peatland rewetting effectively stops these CO2 emissions, but also re-establishes the emission of methane (CH4). Essentially, management must choose between CO2 emissions from drained, or CH4 emissions from rewetted, peatland. This choice must consider radiative effects and atmospheric lifetimes of both gases, with CO2 being a weak but persistent, and CH4 a strong but short-lived, greenhouse gas. The resulting climatic effects are, thus, strongly time-dependent. We used a radiative forcing model to compare forcing dynamics of global scenarios for future peatland management using areal data from the Global Peatland Database. Our results show that CH4 radiative forcing does not undermine the climate change mitigation potential of peatland rewetting. Instead, postponing rewetting increases the long-term warming effect through continued CO2 emissions.
Publisher Correction: Crystal structure of a lipin/Pah phosphatidic acid phosphatase
Authors: Khayyo Valerie I. , Hoffmann Reece M. , Wang Huan , Bell Justin A. , Burke John E. , Reue Karen , Airola Michael V.
Published: April 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
An unperceived acoustic stimulus decreases reaction time to visual information in a patient with cortical deafness
Authors: Carlsen Anthony N. , Maslovat Dana , Kaga Kimitaka
Published: April 2020

Responding to multiple stimuli of different modalities has been shown to reduce reaction time (RT), yet many different processes can potentially contribute to multisensory response enhancement. To investigate the neural circuits involved in voluntary response initiation, an acoustic stimulus of varying intensities (80, 105, or 120 dB) was presented during a visual RT task to a patient with profound bilateral cortical deafness and an intact auditory brainstem response. Despite being unable to consciously perceive sound, RT was reliably shortened (~100 ms) on trials where the unperceived acoustic stimulus was presented, confirming the presence of multisensory response enhancement. Although the exact locus of this enhancement is unclear, these results cannot be attributed to involvement of the auditory cortex. Thus, these data provide new and compelling evidence that activation from subcortical auditory processing circuits can contribute to other cortical or subcortical areas responsible for the initiation of a response, without the need for conscious perception.
Hierarchically Nanoporous Pyropolymers Derived from Waste Pinecone as a Pseudocapacitive Electrode for Lithium Ion Hybrid Capacitors
Authors: Hyun Jong Chan , Kwak Jin Hwan , Lee Sang Moon , Choi Jaewon , Lee Kyu-Tae , Yun Young Soo
Published: April 2020

The non-aqueous asymmetric lithium ion hybrid capacitor (LIHC) is a tactical energy storage device composed of a faradic and non-faradic electrode pair, which aims to achieve both high energy and great power densities. On the other hand, the different types of electrode combinations cause severe imbalances in energy and power capabilities, leading to poor electrochemical performance. Herein, waste pinecone-derived hierarchically porous pyropolymers (WP-HPPs) were fabricated as a surface-driven pseudocapacitive electrode, which has the advantages of both faradic and non-faradic electrodes. The unique materials properties of WP-HPPs possessing high effective surface areas and hierarchically open nanopores led to high specific capacities of ~412 mA h g−1 and considerable rate/cycling performance as a cathode for LIHCs. In particular, nanometer-scale pores, approximately 3 nm in size, plays a key role in the pseudocapacitive charge storage behaviors because open nanopores can transport solvated Li-ions easily into the inside of complex carbon structures and a large specific surface area can be provided by the effective active surface for charge storage. In addition, WP-HPP-based asymmetric LIHCs assembled with a pseudocapacitive counterpart demonstrated feasible electrochemical performance, such as maximum specific energy and specific power of ~340 Wh kg−1 and ~11,000 W kg−1, respectively, with significant cycling stability.
Quantification for non-targeted LC/MS screening without standard substances
Authors: Liigand Jaanus , Wang Tingting , Kellogg Joshua , Smedsgaard Jørn , Cech Nadja , Kruve Anneli
Published: April 2020

Non-targeted and suspect analyses with liquid chromatography/electrospray/high-resolution mass spectrometry (LC/ESI/HRMS) are gaining importance as they enable identification of hundreds or even thousands of compounds in a single sample. Here, we present an approach to address the challenge to quantify compounds identified from LC/HRMS data without authentic standards. The approach uses random forest regression to predict the response of the compounds in ESI/HRMS with a mean error of 2.2 and 2.0 times for ESI positive and negative mode, respectively. We observe that the predicted responses can be transferred between different instruments via a regression approach. Furthermore, we applied the predicted responses to estimate the concentration of the compounds without the standard substances. The approach was validated by quantifying pesticides and mycotoxins in six different cereal samples. For applicability, the accuracy of the concentration prediction needs to be compatible with the effect (e.g. toxicology) predictions. We achieved the average quantification error of 5.4 times, which is well compatible with the accuracy of the toxicology predictions.
Campylobacter gastroenteritis in children in north-eastern Israel comparison with other common pathogens
Authors: Sakran Waheeb , Hexner-Erlichman Zufit , Spiegel Ronen , Batheesh Hamed , Halevy Raphael , Koren Ariel
Published: April 2020

Gastroenteritis is common among children. Campylobacter jejuni is one of the main causative bacterial pathogens, together with Shigella, Salmonella and invasive Escherichia coli. Campylobacteriosis is a zoonotic, usually self-limited disease that does not always require antibiotic treatment. In cases of protracted diarrhoea in healthy children or immunocompromised patients, antibiotic treatment is recommended, and the drug of choice is still macrolides, with very low resistance rates in Campylobacter species. However, it is crucial to isolate the causative organism, because some cases, such as Shigella encephalitis, call for initiation of empiric antibiotic treatment. In this study, we compared the incidence, epidemiology, clinical findings and laboratory results of gastroenteritis with dysentery caused by these organisms in children in our area. C. jejuni was found to be the leading pathogen in children hospitalized with bacterial gastroenteritis, followed by Shigella and Salmonella. Macrolides were the drug of choice for Campylobacter, and ceftriaxone and ciprofloxacin were the best empiric treatments for Shigella and Salmonella, respectively.
Neuro-Musculoskeletal Mapping for Man-Machine Interfacing
Authors: Kapelner Tamas , Sartori Massimo , Negro Francesco , Farina Dario
Published: April 2020

We propose a myoelectric control method based on neural data regression and musculoskeletal modeling. This paradigm uses the timings of motor neuron discharges decoded by high-density surface electromyogram (HD-EMG) decomposition to estimate muscle excitations. The muscle excitations are then mapped into the kinematics of the wrist joint using forward dynamics. The offline tracking performance of the proposed method was superior to that of state-of-the-art myoelectric regression methods based on artificial neural networks in two amputees and in four out of six intact-bodied subjects. In addition to joint kinematics, the proposed data-driven model-based approach also estimated several biomechanical variables in a full feed-forward manner that could potentially be useful in supporting the rehabilitation and training process. These results indicate that using a full forward dynamics musculoskeletal model directly driven by motor neuron activity is a promising approach in rehabilitation and prosthetics to model the series of transformations from muscle excitation to resulting joint function.
Model of Carbon Footprint Assessment for the Life Cycle of the System of Wastewater Collection, Transport and Treatment
Authors: Zawartka Paweł , Burchart-Korol Dorota , Blaut Agata
Published: April 2020

This article presents a model of the environmental assessment of the system of wastewater collection, transport and treatment. The model was developed based on an original environmental assessment method of a system consisting of four elements: septic tanks, household wastewater treatment plants, a sewerage system and a central wastewater treatment plant. To conduct the environmental assessment, the Life Cycle Assessment technique was applied. The Intergovernmental Panel on Climate Change (IPCC) method was also applied, which enabled the determination of the carbon footprint of the analysed wastewater management system. This article presents the outline of an original method applied to create a model and an inventory of the data necessary for environmental assessment and the application of the model for the environmental assessment of a system of wastewater collection, transport and treatment in a city with over 50.000 inhabitants. Three feasible variants (from a functional, technical, organizational and financial point of view) of the system’s development were analysed. The variants were subjected to comparative analysis using the solution. The obtained results, together with the assessment method can be used as a practical tool to assess whether the European Commission’s guidelines are met, as well as the challenges facing wastewater management in the circular economy are overcome.
Crystallographic characterization of a tri-Asp metal-binding site at the three-fold symmetry axis of LarE
Authors: Fellner Matthias , Huizenga Kamren G. , Hausinger Robert P. , Hu Jian
Published: April 2020

Detailed crystallographic characterization of a tri-aspartate metal-binding site previously identified on the three-fold symmetry axis of a hexameric enzyme, LarE from Lactobacillus plantarum, was conducted. By screening an array of monovalent, divalent, and trivalent metal ions, we demonstrated that this metal binding site stoichiometrically binds Ca2+, Mn2+, Fe2+/Fe3+, Co2+, Ni2+, Cu2+, Zn2+, and Cd2+, but not monovalent metal ions, Cr3+, Mg2+, Y3+, Sr2+ or Ba2+. Extensive database searches resulted in only 13 similar metal binding sites in other proteins, indicative of the rareness of tri-aspartate architectures, which allows for engineering such a selective multivalent metal ion binding site into target macromolecules for structural and biophysical characterization.
Optimization of cell viability assays to improve replicability and reproducibility of cancer drug sensitivity screens
Authors: Larsson Peter , Engqvist Hanna , Biermann Jana , Werner Rönnerman Elisabeth , Forssell-Aronsson Eva , Kovács Anikó , Karlsson Per , Helou Khalil , Parris Toshima Z.
Published: April 2020

Cancer drug development has been riddled with high attrition rates, in part, due to poor reproducibility of preclinical models for drug discovery. Poor experimental design and lack of scientific transparency may cause experimental biases that in turn affect data quality, robustness and reproducibility. Here, we pinpoint sources of experimental variability in conventional 2D cell-based cancer drug screens to determine the effect of confounders on cell viability for MCF7 and HCC38 breast cancer cell lines treated with platinum agents (cisplatin and carboplatin) and a proteasome inhibitor (bortezomib). Variance component analysis demonstrated that variations in cell viability were primarily associated with the choice of pharmaceutical drug and cell line, and less likely to be due to the type of growth medium or assay incubation time. Furthermore, careful consideration should be given to different methods of storing diluted pharmaceutical drugs and use of DMSO controls due to the potential risk of evaporation and the subsequent effect on dose-response curves. Optimization of experimental parameters not only improved data quality substantially but also resulted in reproducible results for bortezomib- and cisplatin-treated HCC38, MCF7, MCF-10A, and MDA-MB-436 cells. Taken together, these findings indicate that replicability (the same analyst re-performs the same experiment multiple times) and reproducibility (different analysts perform the same experiment using different experimental conditions) for cell-based drug screens can be improved by identifying potential confounders and subsequent optimization of experimental parameters for each cell line.
Deep microbial proliferation at the basalt interface in 33.5–104 million-year-old oceanic crust
Authors: Suzuki Yohey , Yamashita Seiya , Kouduka Mariko , Ao Yutaro , Mukai Hiroki , Mitsunobu Satoshi , Kagi Hiroyuki , D’Hondt Steven , Inagaki Fumio , Morono Yuki , Hoshino Tatsuhiko , Tomioka Naotaka , Ito Motoo
Published: April 2020

The upper oceanic crust is mainly composed of basaltic lava that constitutes one of the largest habitable zones on Earth. However, the nature of deep microbial life in oceanic crust remains poorly understood, especially where old cold basaltic rock interacts with seawater beneath sediment. Here we show that microbial cells are densely concentrated in Fe-rich smectite on fracture surfaces and veins in 33.5- and 104-million-year-old (Ma) subseafloor basaltic rock. The Fe-rich smectite is locally enriched in organic carbon. Nanoscale solid characterizations reveal the organic carbon to be microbial cells within the Fe-rich smectite, with cell densities locally exceeding 1010 cells/cm3. Dominance of heterotrophic bacteria indicated by analyses of DNA sequences and lipids supports the importance of organic matter as carbon and energy sources in subseafloor basalt. Given the prominence of basaltic lava on Earth and Mars, microbial life could be habitable where subsurface basaltic rocks interact with liquid water.
Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling
Authors: Yoshino Daisuke , Funamoto Kenichi , Sato Kakeru , Kenry  , Sato Masaaki , Lim Chwee Teck
Published: April 2020

Vascular tubulogenesis is tightly linked with physiological and pathological events in the living body. Endothelial cells (ECs), which are constantly exposed to hemodynamic forces, play a key role in tubulogenesis. Hydrostatic pressure in particular has been shown to elicit biophysical and biochemical responses leading to EC-mediated tubulogenesis. However, the relationship between tubulogenesis and hydrostatic pressure remains to be elucidated. Here, we propose a specific mechanism through which hydrostatic pressure promotes tubulogenesis. We show that pressure exposure transiently activates the Ras/extracellular signal-regulated kinase (ERK) pathway in ECs, inducing endothelial tubulogenic responses. Water efflux through aquaporin 1 and activation of protein kinase C via specific G protein–coupled receptors are essential to the pressure-induced transient activation of the Ras/ERK pathway. Our approach could provide a basis for elucidating the mechanopathology of tubulogenesis-related diseases and the development of mechanotherapies for improving human health.
DNA copy number motifs are strong and independent predictors of survival in breast cancer
Authors: Pladsen Arne V. , Nilsen Gro , Rueda Oscar M. , Aure Miriam R. , Borgan Ørnulf , Liestøl Knut , Vitelli Valeria , Frigessi Arnoldo , Langerød Anita , Mathelier Anthony ,   OSBREAC, Bathen Tone F. , Borgen Elin , Børresen-Dale Anne-Lise , Engebråten Olav , Fritzman Britt , Garred Øystein , Geisler Jürgen , Geitvik Gry Aarum , Hofvind Solveig , Kristensen Vessela , Kåresen Rolf , Langerød Anita , Lingjærde Ole Christian , Mælandsmo Gunhild Mari , Naume Bjørn , Russnes Hege G. , Sahlberg Kristine Kleivi , Sauer Torill , Skjerven Helle Kristine , Schlichting Ellen , Sørlie Therese , Engebråten Olav , Kristensen Vessela , Wedge David C. , Van Loo Peter , Caldas Carlos , Børresen-Dale Anne-Lise , Russnes Hege G. , Lingjærde Ole Christian
Published: April 2020

Somatic copy number alterations are a frequent sign of genome instability in cancer. A precise characterization of the genome architecture would reveal underlying instability mechanisms and provide an instrument for outcome prediction and treatment guidance. Here we show that the local spatial behavior of copy number profiles conveys important information about this architecture. Six filters were defined to characterize regional traits in copy number profiles, and the resulting Copy Aberration Regional Mapping Analysis (CARMA) algorithm was applied to tumors in four breast cancer cohorts (n = 2919). The derived motifs represent a layer of information that complements established molecular classifications of breast cancer. A score reflecting presence or absence of motifs provided a highly significant independent prognostic predictor. Results were consistent between cohorts. The nonsite-specific occurrence of the detected patterns suggests that CARMA captures underlying replication and repair defects and could have a future potential in treatment stratification.