bluuebunny/crossref_metadata_2025 huggingface dataset:
Islam and Veterinary Science: Rethinking Animal Suffering Through Islamic Animal Ethics and the Evolving Definition of Halal Slaughter
Authors: Chao En-Chieh
Published: May 2022

In the midst of recent European activism against religious slaughter, the idea that religious slaughter is cruel to animals is often seen as commonsense, and the mandatory pre-slaughter stunning is often portrayed as the moral technology that assures animal welfare. Nevertheless, this portrayal seems to blur the fact that the current notion of animal welfare itself is built upon a changing selection of value assumptions, which are not without problems or academic debates. It also ignores the fact that contemporary veterinary scientists and Muslim scholars have been working together for four decades to learn more about farm animals and their suffering. Despite stereotypes, the idea of animal ethics is not foreign to Islam. In Islam, animals represent God's wisdom and wonder, and humans are obliged to attend to their health and living conditions. When killing animals for food is conducted, the slaughter must be done in the name of God as a sacred ritual in order to assure that the life of the animal is not taken lightly and that the slaughter is not a sign of hostility toward the universe. Before the act of sacrifice, the animal must be healthy, and no harm should be forced upon it. Accordingly, the requirement of pre-slaughter stunning has posed a question to Muslim scholars: Does stunning kill the animal or cause harm? What defines harm, and whose definition counts? This paper reconstructs a socio-technological history of halal slaughter through scientific research on animal suffering since the 1980s. On the basis of archival research of New Zealand veterinary scientists' works and in-depth interviews with Malaysian veterinary scientists, this article outlines three phases of the evolution of halal slaughter that aims to fulfill multiple sets of moral obligations toward farm animals, and demonstrates how veterinary scientists establish common ground between secular and Islamic animal ethics. In this vein, I am envisioning a possibility of veterinary anthropology that recognizes the field's trans-cultural characteristics, and continues to challenge the rigid binaries between the West and the Rest, and between science and culture.
Genomic Sequence and Pathogenicity of the Chicken Anemia Virus Isolated From Chicken in Yunnan Province, China
Authors: Dai Manman , Huang Yuwen , Wang Lin , Luo Jing , Yan Nan , Zhang Lin , Huang Haoqi , Zhou Jie , Li Ziwei , Xu Chenggang
Published: May 2022

Chicken anemia virus (CAV), which has been reported in many countries, causes severe anemia and immunosuppression in chickens. In this study, a CAV strain YN04 belonging to genotype A was first identified from infected chickens in Yunnan province, China. Moreover, the animal infection experiments further confirmed that the strain YN04 is a highly pathogenic strain, which can cause 86.67% mortality in chickens in the infection group. The mean death time of infected chickens was 13.1 days post infection (dpi). CAV infection induced severe anemia with significant decrease in packed cell volume (PCV), and serious atrophy and lesion of thymus and bursa with high viral load at 14 dpi. Besides, CAV infection caused a sharp decrease in chicken body weight and immune organ indices including the ratio of thymus or bursa to body weight at 21 dpi, which displayed the potential immunosuppression state at this stage. These findings enrich the epidemiological data on CAV and may provide information for preventing its further spread in Yunnan province, China.
Complete Genome Analysis of Highly Pathogenic Non-O1/O139 Vibrio cholerae Isolated From Macrobrachium rosenbergii Reveals Pathogenicity and Antibiotic Resistance-Related Genes
Authors: Zhou Yifan , Gu Shuwen , Li Jie , Ji Peng , Zhang Yingjie , Wu Congcong , Jiang Qun , Gao Xiaojian , Zhang Xiaojun
Published: May 2022

Non-O1/O139 Vibrio cholerae is a highly virulent pathogen that causes mass mortalities of various aquatic animals. In the present study, we sequenced the whole genome of non-O1/O139 V. cholerae GXFL1-4, isolated from Macrobrachium rosenbergii, to reveal the pathogenicity and antibiotic resistance. The result showed its genome contained two circular chromosomes and one plasmid with a total size of 4,282,243 bp, which harbored 3,869 coding genes. Among them, 3,047, 2,659, and 3,661 genes were annotated in the Clusters of Orthologous Genes (COG), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG), respectively. In addition, 372 potential virulence genes were predicted based on the Virulence Factor Database (VFDB) database, such as type II, III, IV, and VI secretion systems related genes, flagella genes, and pilus formation or motility-related genes. Blast results in the Comprehensive Antibiotic Resistance Database (CARD) database showed that the strain contained 148 antibiotic resistance-related genes belonging to 27 categories, such as efflux pump complex antibiotic resistance genes and antibiotic resistance gene cluster genes. The Pathogen-Host Interaction (PHI) database annotated 320 genes related to pathogen-host interaction, such as T3SS, virulence regulatory factors, transcriptional regulators, and two-component response regulator related genes. The whole-genome analysis suggested that the pathogenic non-O1/O139 V. cholerae strain GXFL1-4 might have a complex molecular mechanism of pathogenicity and antibiotic resistance. This study provides a wealth of information about non-O1/O139 V. cholerae genes related to its pathogenicity and drug resistance and will facilitate the understanding of its pathogenesis as well as the development of prevention and treatment strategies for the pathogen.
Gene Expression Profile Analysis of Human Epidermal Keratinocytes Expressing Human Papillomavirus Type 8 E7
Authors: Chen Xianzhen , Li Ma , Tang Yi , Liang Qichang , Hua Chunting , He Huiqin , Song Yinjing , Cheng Hao
Published: May 2022

Background: Human papillomavirus type 8 (HPV8) has been implicated in the progress of non-melanoma skin cancers and their precursor lesions. The HPV8 E7 oncoprotein plays a key role in the tumorigenesis of HPV-associated cutaneous tumors. However, the exact role of HPV8 E7 in human epidermal carcinogenesis has not been fully elucidated.

Methods: To investigate the potential carcinogenic effects of HPV8 E7 on epithelial cells, we used RNA-sequencing technology to analyze the gene expression profile of HPV8 E7-overexpressed normal human epidermal keratinocytes (NHEKs).

Results: RNA-sequencing revealed 831 differentially expressed genes (DEGs) between HPV8 E7-expressing NHEKs and control cells, among which, 631 genes were significantly upregulated, and 200 were downregulated. Gene ontology annotation enrichment analysis showed that HPV8 E7 mainly affected the expression of genes associated with protein heterodimerization activity, DNA binding, nucleosomes, and nucleosome assembly. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that overexpression of HPV8 E7 affected the expression of gene clusters associated with viral carcinogenesis and transcriptional misregulation in cancer and necroptosis signaling pathways that reportedly play crucial roles in HPV infection promotion and cancer progression. We also found the DEGs, such as HKDC1 and TNFAIP3, were associated with epigenetic modifications, immune regulation, and metabolic pathways.

Conclusion: Our results demonstrate that the pro-carcinogenic effect of HPV8 expression in epithelial cells may be attributed to the regulatory effect of oncogene E7 on gene expression associated with epigenetic modifications and immune and metabolic status-associated gene expression. Although our data are based on an in vitro experiment, it provides the theoretical evidence that the development of squamous cell carcinoma can be caused by HPV.
Distribution and Clinical Significance of IL-17A in Tumor-Infiltrating Lymphocytes of Non-Small Cell Lung Cancer Patients
Authors: Xu Rui , Ke Xing , Shang Wenwen , Liu Shuna , Fu Xin , Wang Ting , Jin Shuxian
Published: May 2022

Objective: To investigate the distribution of IL-17A and its clinical significance in tumor infiltrating lymphocytes (TILs) of patients with non-small cell lung cancer (NSCLC).

Methods: Expression level of IL-17A in TILs of 3 paired NSCLC and paracancerous specimens was measured by qRT-PCR. The distribution of IL-17A in immune cell subsets of 15 paired NSCLC and paracancerous specimens was examined by flow cytometry. The correlation between IL-17A and clinical features of NSCLC was identified.

Results: IL-17A was significantly upregulated in TILs of NSCLC specimens than those of paracancerous ones (p < 0.0001). Meanwhile, T helper 17 cells (Th17 cells, p < 0.001), IL-17-secreting CD8+ T cells (Tc17 cells, p < 0.001) and IL-17-producing cells (γδT17 cells, p < 0.0001) were significantly abundant in TILs of NSCLC specimens than those of controls, and the higher abundance of the latter was much pronounced than that of the former two. Moreover, γδT17 cells in TILs were significantly correlated with lymphatic metastasis and CYFRA 21-1 level of NSCLC patients (p < 0.05).

Conclusion: Tumor infiltrated γδT cells are the main source of IL-17 in early-stage NSCLC, and IL-17 may be a vital regulator involved in the development of NSCLC.
Donor-Derived Cell-Free DNA for the Detection of Heart Allograft Injury: The Impact of the Timing of the Liquid Biopsy
Authors: Verhoeven Jeroen G. H. P. , Hesselink Dennis A. , Peeters Annemiek M. A. , de Jonge Evert , von der Thüsen Jan H. , van Schaik Ron H. N. , Matic Maja , Baan Carla C. , Manintveld O. C. , Boer Karin
Published: May 2022

Background: In heart transplant recipients, donor-derived cell-free DNA (ddcfDNA) is a potential biomarker for acute rejection (AR), in that increased values may indicate rejection. For the assessment of ddcfDNA as new biomarker for rejection, blood plasma sampling around the endomyocardial biopsy (EMB) seems a practical approach. To evaluate the effect of the EMB procedure on ddcfDNA values, ddcfDNA values before the EMB were pairwise compared to ddcfDNA values after the EMB. We aimed at evaluating whether it matters whether the ddcfDNA sampling is done before or after the EMB-procedure.

Methods: Plasma samples from heart transplant recipients were obtained pre-EMB and post-EMB. A droplet digital PCR method was used for measuring ddcfDNA, making use of single-nucleotide polymorphisms that allowed both relative quantification, as well as absolute quantification of ddcfDNA.

Results: Pairwise comparison of ddcfDNA values pre-EMB with post-EMB samples (n = 113) showed significantly increased ddcfDNA concentrations and ddcfDNA% in post-EMB samples: an average 1.28-fold increase in ddcfDNA concentrations and a 1.31-fold increase in ddcfDNA% was observed (p = 0.007 and p = 0.03, respectively).

Conclusion: The EMB procedure causes iatrogenic injury to the allograft that results in an increase in ddcfDNA% and ddcfDNA concentrations. For the assessment of ddcfDNA as marker for AR, collection of plasma samples before the EMB procedure is therefore essential.
Plasma Biomarkers for Clinical Assessment of Bone Mineral Density in Heart Transplanted Patients—A Single-Center Study at Skåne University Hospital in Lund
Authors: Löfdahl Eveline , Ahmed Salaheldin , Ahmed Abdulla , Rådegran Göran
Published: May 2022

We aimed to identify plasma biomarkers that predict changes in bone mineral density (BMD) and increase the understanding of impaired BMD after heart transplantation (HT). Twenty-eight adult patients were included. Data, including densitometry and 29 plasma proteins, before and 1 year after HT were analyzed. Pre-HT plasma levels of fibroblast growth factor 23 (FGF23) correlated with post-HT T score in lumbar spine, adjusted for age, gender, and BMI (1.72 [95% CI 1.33; 2.22], p = 0.011). Change (∆; post-HT—pre-HT) in plasma levels of melusin correlated to ∆T score from the lumbar spine (p = 0.028). ∆plasma levels of TR-AP, ITGB2, and Stromelysin-1 correlated to ∆T score from the femoral neck (p < 0.05). However, no correlations remained after adjustments for age, gender, and BMI. In conclusion, elevated plasma FGF23 pre-HT predicted an increase in lumbar BMD after HT. However, the results are surprising since FGF23 is known to be inversely correlated with BMD. This may partly be explained by the complex pathophysiology in this particular cohort. Due to the explorative nature of the study and the small sample size, further investigations of biochemical markers on bone metabolism in this patient population are encouraged.
A Novel Method of CD31-Combined ABO Carbohydrate Antigen Microarray Predicts Acute Antibody-Mediated Rejection in ABO-Incompatible Kidney Transplantation
Authors: Tasaki Masayuki , Tateno Hiroaki , Sato Takashi , Tomioka Azusa , Kaji Hiroyuki , Narimatsu Hisashi , Saito Kazuhide , Nakagawa Yuki , Aoki Toshinari , Kamimura Masami , Ushiki Takashi , Okada Manabu , Miwa Yuko , Hotta Kiyohiko , Yoshida Yutaka , Takahashi Kota , Tomita Yoshihiko
Published: May 2022

Isohemagglutinin assays employing red blood cells (RBCs) are the most common assays used to measure antibody titer in ABO-incompatible kidney transplantation (ABOi KTx). However, ABO antigens expressed on RBCs are not identical to those of kidney and antibody titers do not always correlate with clinical outcome. We previously reported that CD31 was the main protein linked to ABO antigens on kidney endothelial cells (KECs), which was different from those on RBCs. We developed a new method to measure antibody titer using a microarray of recombinant CD31 (rCD31) linked to ABO antigens (CD31-ABO microarray). Mass spectrometry analysis suggested that rCD31 and native CD31 purified from human kidney had similar ABO glycan. To confirm clinical use of CD31-ABO microarray, a total of 252 plasma samples including volunteers, hemodialysis patients, and transplant recipients were examined. In transplant recipients, any initial IgG or IgM antibody intensity >30,000 against the donor blood type in the CD31-ABO microarray showed higher sensitivity, specificity, positive predictive value, and negative predictive value of AABMR, compared to isohemagglutinin assays. Use of a CD31-ABO microarray to determine antibody titer specifically against ABO antigens expressed on KECs will contribute to precisely predicting AABMR or preventing over immunosuppression following ABOi KTx.
Information Needs of People With Limited Health Literacy Regarding a New “Opt-Out” Organ Donation System: A Qualitative Study in the Netherlands
Authors: Rademakers Jany , Rolink Marlon , Heijmans Monique
Published: May 2022

Graphical Abstract
Coordinated Formation Guidance Law for Fixed-Wing UAVs Based on Missile Parallel Approach Method
Authors: Gong Zheng , Zhou Zan , Wang Zian , Lv Quanhui , Xu Jinfa , Jiang Yunpeng
Published: May 2022

This paper presents a classic missile-type parallel-approach guidance law for fixed-wing UAVs in coordinated formation flight. The key idea of the proposed guidance law is to drive each follower to follow the virtual target point. Considering the turning ability of each follower, the formation form adopts the semi-perfect rigid form, which does not require the vehicle positions form a rigid formation, and the orientations keep consensus. According to the mission characteristics of the follower following a leader and the leader following a route, three guidance laws for straight, turning, and circling flight are designed. A series of experiments demonstrate the proposed guidance law’s improved response and maneuvering stability. The results of hardware-in-the-loop simulations and real flight tests prove that the proposed guidance law satisfies the practical UAV formation flight control demands.
Performance Evaluation of Multiflight Ground Handling Process
Authors: Li Biao , Wang Liwen , Xing Zhiwei , Luo Qian
Published: May 2022

The ever-increasing high density of flights arouses an urgent requirement to improve the effectiveness and performance of ground handling in airport operation. The implementation of coordinated airport decision-making quantifies the ground handling process into a series of key milestone nodes, which is more conducive for operators to reduce resource consumption and flight delays. An innovative performance evaluation method for the multiflight ground handling process is proposed based on shared information of milestone nodes in the ground handling. A dynamic performance evaluation model is established, which should superimpose the performance evaluation results of the single-flight ground handling process. Meanwhile, the indicators and weights of the single-flight performance evaluation are obtained by combining the ground handling process prediction and expected value. As time evolves, a matrix method for the multiflight ground handling performance evaluation is proposed to combine the logic and evolution of the process. It is shown that the average prediction accuracy of single-flight ground handling process nodes can be increased to 87.63%. The experimental analysis demonstrates that the objectivity, effectiveness and dynamics of the proposed approach can be the basis for short-term tactics in airport.
Spatial and In-Depth Distribution of Soil Salinity and Heavy Metals (Pb, Zn, Cd, Ni, Cu) in Arable Irrigated Soils in Southern Kazakhstan
Authors: Suska-Malawska Małgorzata , Vyrakhamanova Assem , Ibraeva Marya , Poshanov Maksat , Sulwiński Marcin , Toderich Kristina , Mętrak Monika
Published: May 2022

Most irrigated lands in the Republic of Kazakhstan are in its southern part, in the large deltas and ancient alluvial plains in the basins of the rivers Syr Darya and Ili. The combination of climatic features and anthropogenic pressures leads to increased salinity and contamination of cultivated soils in this region, resulting in a qualitative and quantitative decline in crop production. The study’s primary goal was to assess soil secondary salinity and selected heavy metals (Pb, Zn, Cd, Ni and Cu) contamination in irrigated arable soils. To identify the potential source of soil pollution, we compared the concentration of salt and heavy metals (both total and mobile forms) in different soil types in three depths of soil profiles obtained from irrigated cultivated and non-cultivated (abounded) territory in the Shauldara massif in the southern part of Kazakhstan. All studied soils are prone to secondary salinization with either a medium or high content of sum of salts with domination by Na+ among cations and by SO42− among anions. The soil contamination with heavy metals was low, and, in most cases, except for cadmium, it was below the limits developed for arable soils in most countries. Soil contamination with cadmium results from contamination of the water used for irrigation of farmland.
In Vitro Induction and Primary Evaluation of Octoploid Plants in Saskatoon Berry (Amelanchier alnifolia Nutt.)
Authors: Kucharska Danuta , Podwyszyńska Małgorzata , Trzewik Aleksandra , Marasek-Ciołakowska Agnieszka , Pluta Stanisław , Seliga Łukasz
Published: May 2022

Polyploids of many species of horticultural plants are widely used in breeding programs since they are characterized by vigorous growth, larger organs, and greater resistance to biotic and abiotic stress. Saskatoon berry (Amelanchier alnifolia Nutt.) is in a form of diploid (2n = 2x = 34) and tetraploid. So far, no reports on induction of polyploidy in this species have been found. The aim of the study was to obtain Saskatoon berry octoploids with the use of in vitro shoot cultures. Shoots of the tetraploid of Canadian cultivars ‘Martin’ and ‘Smoky’ derived from in vitro cultures were used for induction of chromosome doubling. The shoot explants were incubated in a multiplication medium with the addition of one of the following antimitotics: colchicine, trifluralin, oryzalin or amiprophos methyl (APM) for two weeks (six days in the darkness and then eight days under a 16-h photoperiod). Then, the shoots were transferred to the multiplication medium without antimitotics and propagated in vitro by two subcultures. Octoploids were selected based on flow cytometry. Trifluralin showed the most phytotoxic effect. Six octoploids were obtained, four for ‘Martin’ after treatment with 250 mg L−1 of colchicine and two for ‘Smoky’ following the treatment with 5 mg L−1 of APM. All obtained octoploid individuals were multiplied and the level of ploidy was re-examined by flow cytometry and chromosome counting, which confirmed their octoploid genotype (2n = 8x = 136). They are probably the first octoploids of the genus Amelanchier in the world. The newly obtained octoploids were rooted in vitro, acclimatized to ex vitro conditions and grown in a greenhouse. Octoploids grew very slowly and showed a tendency to premature dormancy, which was manifested by inhibition of growth. In octoploid plants, the stomata were significantly larger and the number of stomata per 1 mm2 of leaf area was lower, compared to the tetraploid counterparts.
Improving the Authorization of Microbial Biological Control Products (MBCP) in the European Union within the EU Green Deal Framework
Authors: Helepciuc Florența-Elena , Todor Arpad
Published: May 2022

Developing sustainable agriculture by identifying non-chemical alternative Plant Protection Products (PPP) is a cornerstone in achieving long-sought environmental friendliness. Despite significant legislative and political efforts to promote biocontrol solutions and Integrated Pest Management (IPM), the literature points out the disadvantages posed by European Union’s (EU) two-tier system for Microbial Biological Control Agents (MBCA) approval and subsequent Microbial Biological Control Products (MBCP) authorization by each EU Member State (MS). Despite the disadvantages, in a recent article, we showed that the EU had outcompeted the US and other countries in approved MBCA in the last decades; however, MBCP approval at the national level lags. Achieving the EU Green Deal’s aim set out in the ‘Farm to Fork Strategy’ to reduce the use and risk of pesticides by 50% by 2030 is difficult without developing viable alternatives. Why do we not have higher MBCP availability and usage in the EU? Is it the current legislation, its poor application, or some other factors? The current legislative framework stimulated MBCA approval. Thus, we compare MBCA approval and MBCP authorization procedure to evaluate if MBCP authorization is more difficult and thus causes a bottleneck. We find that requirements for MBCP authorization are unnecessarily more complex. We recommend simplifying the MBCP dossier requirements and making them as similar to MBCA as possible to accelerate the MBCP authorization in more EU MS to increase their availability and integration in agronomic crops’ pest management plans.
Effective Field Immobilisation and Capture of Giraffe (Giraffa camelopardalis)
Authors: Deacon Francois , Daffue Willem , Nel Pierre , Higgs Ruan
Published: May 2022

One of the highest occurrences of mortalities among giraffes (Giraffa camelopardalis) takes place during immobilisations, captures and translocations. Common mistakes, human error, unforeseen risks, the awkward anatomy and the sheer size of the animal are leading factors for giraffes’ mortalities during these operations. Many risks can be circumvented but some risks are unpreventable, often due to terrain characteristics (rivers, deep ditches, holes and rocky terrain). From 2011 to 2021, seventy-five giraffes were successfully immobilised and captured to collect biological and physiological data from eight different study areas across South Africa. A 0% mortality and injury rate was achieved and, therefore, the techniques described in this paper are testimony to the advances and improvements of capture techniques and drugs. Biological information and capture experiences were noted for 75 immobilised giraffes, of which, knockdown time data were recorded for 43 individuals. Effective and safe immobilisation requires a competent team, proper planning, skill and knowledge. In this manuscript, we address procedures, techniques, ethical compliance, welfare and safety of the study animals. General experiences and lessons learned are also shared and should benefit future captures and immobilisations by limiting the risks involved. The sharing of experiences and information could influence and improve critical assessments of different capture techniques and can likely contribute to the success rate of immobilisation and translocation success for giraffes in the future.
Impact of Endemic Besnoitiosis on the Performance of a Dairy Cattle Herd
Authors: Anastácio Catarina , Bexiga Ricardo , Nolasco Sofia , Zúquete Sara , Delgado Inês L. S. , Nunes Telmo , Leitão Alexandre
Published: May 2022

This study aimed to assess the effect of Besnoitia besnoiti infection on the reproductive and productive performance of a dairy cattle herd. A serological screening was performed by indirect fluorescent antibody test (IFAT) on every animal aged over one year (n = 262). Subsequently, 211 animals were clinically examined, with 96 of those being screened for detection of sclerocysts. The overall seroprevalence was 62.9% (CI95%: 56.1–69.5%). On clinical examination, 7.6% (16/211) of the animals presented chronic skin lesions, and 47.9% (46/96) had sclerocysts. Multivariate logistic regression showed that the time on herd represented a risk factor, and the odds of acquiring the infection increased 1.683× per additional year on herd, ranging from less than a year to 8 years. Seropositivity and the presence of sclerocysts revealed an association with a higher milk somatic cell count, which may have a considerable economic impact on dairy production. Regarding reproductive indicators, no negative impact could be associated with clinical besnoitiosis or positive serological results. In conclusion, our study highlights the need to thoroughly evaluate the economic impact of this emerging disease in dairy herd production to help with decision making at both herd and regional levels, particularly in endemic areas.
Effect of Microbial Phytase on Ileal Digestibility of Minerals, Plasma and Urine Metabolites, and Bone Mineral Concentrations in Growing–Finishing Pigs
Authors: Czech Anna , Samolińska Wioletta , Tomaszewska Ewa , Muszyński Siemowit , Grela Eugeniusz R.
Published: May 2022

The study was conducted to evaluate the effects of added phytase in the diet of pigs on utilization of minerals and to determine the essential levels of this supplement in compound feed. An additional aim of the study was a critical assessment of current recommendations regarding the level of phosphorus in the diet of pigs, taking into account the use of phytase. A total of 432 pigs were allotted to six dietary treatments, with nine replicate pens per treatment according to body weight (BW) and sex. The treatments included a negative control (NC) with reduced content of digestible phosphorus; the NC diet supplemented with 6-phytase produced by a genetically modified strain of Aspergillus oryzae in the amount of 250 (NC + 250), 500 (NC + 500), 1000 (NC + 1000), or 1500 (NC + 1500) FTU/kg of feed; and a positive control (PC) diet formulated to meet NCR nutrient requirements for pigs. The results showed that, the higher the phytase activity in the diet (NC+), the lower the concentration of P, Ca, and Mg in the chyme (p < 0.05). Pigs fed the PC and NC+ diets had higher plasma levels of P and Ca than group NC in both fattening periods (p < 0.05). The content of phosphorus in the femur of pigs increased with the level of phytase added to the diet (p < 0.05). The content of Cu and Zn in the femur of pigs in the growing period was higher in groups NC + 500 and NC + 1000 than in the remaining experimental groups (p < 0.001). The content of P and Ca in the feces was higher in pigs fed the PC diet in comparison to the remaining experimental groups in both fattening periods (p < 0.001). There was a decrease in the content of P, Ca, and Mg in the excreta of pigs fed NC+ diets in both fattening periods (p < 0.05). A linear decrease in excretion of zinc in the feces was noted in the case of high levels of phytase, i.e., 1000 and 1500 FTU (p < 0.001). Increasing the level of phytase decreased the Cu (p < 0.001) content in the urine of growing–finishing pigs. In conclusion, the analysis of the effects of the use of phytase in a range of 0–1500 FTU/kg in low-phosphorus diets for fattening pigs indicates that 1000 FTU is the most effective level of phytase for increasing utilization of minerals and reducing excretion of elements into the environment.
Measurement Duration but Not Distance, Angle, and Neighbour-Proximity Affects Precision in Enteric Methane Emissions when Using the Laser Methane Detector Technique in Lactating Dairy Cows
Authors: Boré Raphaël , Bruder Thiphaine , El Jabri Mohammed , March Margaret , Hargreaves Paul R. , Rouillé Benoît , Dewhurst Richard J. , Chagunda Mizeck G. G.
Published: May 2022

The laser methane detector (LMD), is a proprietary hand-held open path laser measuring device. Its measurements are based on infrared absorption spectroscopy using a semiconductor laser as a collimated excitation source. In the current study, LMD measurements were carried out in two experiments using 20 and 71 lactating dairy cows in Spain and Scotland, respectively. The study aimed at testing four assumptions that may impact on the reliability and repeatability of the LMD measurements of ruminants. The study has verified that there is no difference in enteric methane measurements taken from a distance of 3 m than from those taken at a distance of 2 m; there was no effect to the measurements when the measurement angle was adjusted from 90° to 45°; that the presence of an adjacent animal had no effect on the methane measurements; and that measurements lasting up to 240 s are more precise than those taken for a shorter duration. The results indicate that angle, proximity to other animals, and distance had no effects and that measurements need to last a minimum of 240 s to maintain precision.
Risk Factors for Exercise-Associated Sudden Cardiac Death in Thoroughbred Racehorses
Authors: Nath Laura , Stent Andrew , Elliott Adrian , La Gerche Andre , Franklin Samantha
Published: May 2022

Cardiac arrhythmias resulting in sudden cardiac death (SCD) are an important cause of racehorse fatalities. The objective of this study was to determine risk factors for SCD in Thoroughbreds by evaluating a sample with a policy of mandatory post-mortem following racing or training fatalities. Risk factors were compared between case horses with SCD (n = 57) and control horses with other fatal injury (OFI, n = 188) by univariable and multivariable logistic regression. Survival in years for horses with SCD was compared to OFI using the Kaplan–Meier method with log rank test. The following variables were most important in the multiple logistic model: Horses with SCD were more likely to die during training than during racing, SCD (42/57, 74%) vs. OFI (82/188, 44%; odds ratio [OR], 95% confidence interval [CI], 2.5, 1.2–5.4; p = 0.01), had fewer lifetime starts, median (interquartile range [IQR]), SCD (3.0 [0.0–9.0]) vs. OFI (9.0 [0.0–22.8]; OR, 95% CI, 0.96, 0.9–1.0; p = 0.02 and were less likely to be entire (uncastrated) males, SCD 9/57 (16%) vs. OFI (46/188, 25%; OR, 95% CI, 0.47, 0.1–0.9; p = 0.03). Survival in years (median (IQR)) for horses with SCD was 3.6 (3.1–4.4), which was shorter than OFI (4.5 [3.1–6.0], hazard ratio, 95%CI, 1.6,1.2–2.3; p < 0.001). SCD occurs more commonly in training than racing, which suggests exercise intensity is less important in precipitating this fatality. In this study, SCD occurred early in the careers of affected horses.
PM2.5 Air Pollution Prediction through Deep Learning Using Multisource Meteorological, Wildfire, and Heat Data
Authors: Muthukumar Pratyush , Nagrecha Kabir , Comer Dawn , Calvert Chisato Fukuda , Amini Navid , Holm Jeanne , Pourhomayoun Mohammad
Published: May 2022

Air pollution is a lethal global threat. To mitigate the effects of air pollution, we must first understand it, find its patterns and correlations, and predict it in advance. Air pollution is highly dependent on spatial and temporal correlations of prior meteorological, wildfire, and pollution structures. We use the advanced deep predictive Convolutional LSTM (ConvLSTM) model paired with the cutting-edge Graph Convolutional Network (GCN) architecture to predict spatiotemporal hourly PM2.5 across the Los Angeles area over time. Our deep-learning model does not use atmospheric physics or chemical mechanism data, but rather multisource imagery and sensor data. We use high-resolution remote-sensing satellite imagery from the Moderate Resolution Imaging Spectroradiometer (MODIS) instrument onboard the NASA Terra+Aqua satellites and remote-sensing data from the Tropospheric Monitoring Instrument (TROPOMI), a multispectral imaging spectrometer onboard the Sentinel-5P satellite. We use the highly correlated Fire Radiative Power data product from the MODIS instrument which provides valuable information about the radiant heat output and effects of wildfires on atmospheric air pollutants. The input data we use in our deep-learning model is representative of the major sources of ground-level PM2.5 and thus we can predict hourly PM2.5 at unparalleled accuracies. Our RMSE and NRMSE scores over various site locations and predictive time frames show significant improvement over existing research in predicting PM2.5 using spatiotemporal deep predictive algorithms.
Prediction of Fine Particulate Matter Concentration near the Ground in North China from Multivariable Remote Sensing Data Based on MIV-BP Neural Network
Authors: Wu Hailing , Zhang Ying , Li Zhengqiang , Wei Yuanyuan , Peng Zongren , Luo Jie , Ou Yang
Published: May 2022

Rapid urbanization and industrialization lead to severe air pollution in China, threatening public health. However, it is challenging to understand the pollutants’ spatial distributions by relying on a network of ground-based monitoring instruments, considering the incomplete dataset. To predict the spatial distribution of fine-mode particulate matter (PM2.5) pollution near the surface, we established models based on the back propagation (BP) neural network for PM2.5 mass concentration in North China using remote sensing products. According to our predictions, PM2.5 mass concentrations are affected by changes in surface reflectance and the dominant particle size for different seasons. The PM2.5 mass concentration predicted by the seasonal model shows a similar spatial pattern (high in the east but low in the west) influenced by the terrain, but shows high value in winter and low in summer. Compared to the ground-based data, our predictions agree with the spatial distribution of PM2.5 mass concentrations, with a mean bias of +17% in the North China Plain in 2017. Furthermore, the correlation coefficients (R) of the four seasons’ instantaneous measurements are always above 0.7, indicating that the seasonal models primarily improve the PM2.5 mass concentration prediction.
On the Detection of Snow Cover Changes over the Australian Snowy Mountains Using a Dynamic OBIA Approach
Authors: Rasouli Aliakbar A. , Cheung Kevin K. W. , Mohammadzadeh Alajujeh Keyvan , Ji Fei
Published: May 2022

This study detected the spatial changes in Snow Cover Area (SCA) over the Snowy Mountains in New South Wales, Australia. We applied a combination of Object-Based Image Analysis (OBIA) algorithms by segmentation, classification, and thresholding rules to extract the snow, water, vegetation, and non-vegetation land covers. For validation, the Maximum Snow Depths (MSDs) were collected at three local snow observation sites (namely Three Mile Dam, Spencer Creek, and Deep Creek) from 1984 to 2020. Multiple Landsat 5, 7, and 8 imageries extracted daily MSDs. The process was followed by applying an Estimation Scale Parameter (ESP) tool to build the local variance (LV) of object heterogeneity for each satellite scene. By matching the required segmentation parameters, the optimal separation step of the image objects was weighted for each of the image bands and the Digital Elevation Model (DEM). In the classification stage, a few land cover classes were initially assigned, and three different indices—Normalized Differential Vegetation Index (NDVI), Surface Water Index (SWI), and a Normalized Differential Snow Index (NDSI)—were created. These indices were used to adjust a few classification thresholds and ruleset functions. The resulting MSDs in all snow observation sites proves noticeable reduction trends during the study period. The SCA classified maps, with an overall accuracy of nearly 0.96, reveal non-significant trends, although with considerable fluctuations over the past 37 years. The variations concentrate in the north and south-east directions, to some extent with a similar pattern each year. Although the long-term changes in SCA are not significant, since 2006, the pattern of maximum values has decreased, with fewer fluctuations in wet and dry episodes. A preliminary analysis of climate drivers’ influences on MSD and SCA variability has also been performed. A dynamic indexing OBIA indicated that continuous processing of satellite images is an effective method of obtaining accurate spatial–temporal SCA information, which is critical for managing water resources and other geo-environmental investigations.
Non-Stationary Hydrological Regimes Due to Climate Change: The Impact of Future Precipitation in the Spillway Design of a Reservoir, Case Study: Sube y Baja Dam, in Ecuador
Authors: Herbozo Jorge Enrique , Muñoz Luis Eduardo , Guerra María José , Minaya Veronica , Haro Patricia , Carrillo Veronica , Manciati Carla , Campozano Lenin
Published: May 2022

Changes in flood loads and reservoir levels, produced by climate change (CC), represent an increasing concern for dam safety managers and downstream populations, highlighting the need to define adaptation strategies based on the dam failure risk management framework. Currently, thousands of dams worldwide, varying in use, age, and maintenance, may represent a threat to downstream cities in the case of structural failure. Several studies relate the failure of dams to several issues in the spillway, which may be even more vulnerable in CC conditions. This study provides a review of dam safety threats due to CC and approaches for the design/redesign of the spillway to cope with CC. A general four-stage methodology is proposed: data gathering and hydro-climatic, hydrological, and hydraulic analyses. Afterward, this methodology is applied to the spillway design for the Sube y Baja dam in Ecuador. The Probable Maximum Precipitation (PMP) increases around 20% considering CC under the Representative Concentration Pathway 8.5. Such an increment derived a 25% increase in the spillway maximum flow. These results show that the non-stationary hydrological regimes related to CC require a revision of engineering design criteria for hydraulic structures in general, and call for a consensus on design variables under CC.
How Paretic and Non-Paretic Ankle Muscles Contract during Walking in Stroke Survivors: New Insight Using Novel Wearable Ultrasound Imaging and Sensing Technology
Authors: Lyu Pei-Zhao , Zhu Ringo Tang-Long , Ling Yan To , Wang Li-Ke , Zheng Yong-Ping , Ma Christina Zong-Hao
Published: May 2022

Abnormal muscle tone and muscle weakness are related to gait asymmetry in stroke survivors. However, the internal muscle morphological changes that occur during walking remain unclear. To address this issue, this study investigated the muscle activity of the tibialis anterior (TA) and medial gastrocnemius (MG) of both the paretic and non-paretic sides during walking in nine stroke survivors, by simultaneously capturing electromyography (EMG), mechanomyography (MMG), and ultrasound images, and using a validated novel wearable ultrasound imaging and sensing system. Statistical analysis was performed to examine the test–retest reliability of the collected data, and both the main and interaction effects of each “side” (paretic vs. non-paretic) and “gait” factors, in stroke survivors. This study observed significantly good test–retest reliability in the collected data (0.794 ≤ ICC ≤ 0.985), and significant differences existed in both the side and gait factors of the average TA muscle thickness from ultrasound images, and in the gait factors of TA and MG muscle’s MMG and EMG signals (p < 0.05). The muscle morphological characteristics also appeared to be different between the paretic and non-paretic sides on ultrasound images. This study uncovered significantly different internal muscle contraction patterns between paretic and non-paretic sides during walking for TA (7.2% ± 1.6%) and MG (5.3% ± 4.9%) muscles in stroke survivors.
Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture
Authors: Wei Chunyang , Yu Chengzhuang , Li Shanshan , Li Tiejun , Meng Jiyu , Li Junwei
Published: May 2022

Cell culture plays an essential role in tissue engineering and high-throughput drug screening. Compared with two-dimensional (2D) in vitro culture, three-dimensional (3D) in vitro culture can mimic cells in vivo more accurately, including complex cellular organizations, heterogeneity, and cell–extracellular matrix (ECM) interactions. This article presents a droplet-based microfluidic chip that integrates cell distribution, 3D in vitro cell culture, and in situ cell monitoring in a single device. Using the microfluidic “co-flow step emulsification” approach, we have successfully prepared close-packed droplet arrays with an ultra-high-volume fraction (72%) which can prevent cells from adhering to the chip surface so as to achieve a 3D cell culture and make scalable and high-throughput cell culture possible. The proposed device could produce droplets from 55.29 ± 1.52 to 95.64 ± 3.35 μm, enabling the diverse encapsulation of cells of different sizes and quantities. Furthermore, the cost for each microfluidic CFSE chip is approximately USD 3, making it a low-cost approach for 3D cell culture. The proposed device is successfully applied in the 3D culture of saccharomyces cerevisiae cells with an occurrence rate for proliferation of 80.34 ± 3.77%. With low-cost, easy-to-operate, high-throughput, and miniaturization characteristics, the proposed device meets the requirements for 3D in vitro cell culture and is expected to be applied in biological fields such as drug toxicology and pharmacokinetics.
Fiber-Optic Distributed Sensing Network for Thermal Mapping of Gold Nanoparticles-Mediated Radiofrequency Ablation
Authors: Sametova Akbota , Kurmashev Sabit , Ashikbayeva Zhannat , Amantayeva Aida , Blanc Wilfried , Atabaev Timur Sh. , Tosi Daniele
Published: May 2022

In this work, we report the design of an optical fiber distributed sensing network for the 2-dimensional (2D) in situ thermal mapping of advanced methods for radiofrequency thermal ablation. The sensing system is based on six high-scattering MgO-doped optical fibers, interleaved by a scattering-level spatial multiplexing approach that allows simultaneous detection of each fiber location, in a 40 × 20 mm grid (7.8 mm2 pixel size). Radiofrequency ablation (RFA) was performed on bovine phantom, using a pristine approach and methods mediated by agarose and gold nanoparticles in order to enhance the ablation properties. The 2D sensors allow the detection of spatiotemporal patterns, evaluating the heating properties and investigating the repeatability. We observe that agarose-based ablation yields the widest ablated area in the best-case scenario, while gold nanoparticles-mediated ablation provides the best trade-off between the ablated area (53.0–65.1 mm2, 61.5 mm2 mean value) and repeatability.
Quantum Mechanical Aspects in the Pathophysiology of Neuropathic Pain
Authors: Nawafleh Sager , Qaswal Abdallah Barjas , Alali Obada , Zayed Fuad Mohammed , Al-Azzam Ahmed Mahmoud , Al-Kharouf Khaled , Ali Mo’ath Bani , Albliwi Moath Ahmad , Al-Hamarsheh Rawan , Iswaid Mohammad , Albanna Ahmad , Enjadat Ahmad , Al-Adwan Mohammad Abu Orabi , Dibbeh Khaled , Shareah Ez-Aldeen Abu , Hamdan Anas , Suleiman Aiman
Published: May 2022

Neuropathic pain is a challenging complaint for patients and clinicians since there are no effective agents available to get satisfactory outcomes even though the pharmacological agents target reasonable pathophysiological mechanisms. This may indicate that other aspects in these mechanisms should be unveiled to comprehend the pathogenesis of neuropathic pain and thus find more effective treatments. Therefore, in the present study, several mechanisms are chosen to be reconsidered in the pathophysiology of neuropathic pain from a quantum mechanical perspective. The mathematical model of the ions quantum tunneling model is used to provide quantum aspects in the pathophysiology of neuropathic pain. Three major pathophysiological mechanisms are revisited in the context of the quantum tunneling model. These include: (1) the depolarized membrane potential of neurons; (2) the cross-talk or the ephaptic coupling between the neurons; and (3) the spontaneous neuronal activity and the emergence of ectopic action potentials. We will show mathematically that the quantum tunneling model can predict the occurrence of neuronal membrane depolarization attributed to the quantum tunneling current of sodium ions. Moreover, the probability of inducing an ectopic action potential in the axons of neurons will be calculated and will be shown to be significant and influential. These ectopic action potentials are generated due to the formation of quantum synapses which are assumed to be the mechanism behind the ephaptic transmission. Furthermore, the spontaneous neuronal activity and the emergence of ectopic action potentials independently from any adjacent stimulated neurons are predicted to occur according to the quantum tunneling model. All these quantum mechanical aspects contribute to the overall hyperexcitability of the neurons and to the pathogenesis of neuropathic pain. Additionally, providing a new perspective in the pathophysiology of neuropathic pain may improve our understanding of how the neuropathic pain is generated and maintained and may offer new effective agents that can improve the overall clinical outcomes of the patients.
The Effects of Attention on the Syllable-Induced Prepulse Inhibition of the Startle Reflex and Cortical EEG Responses against Energetic or Informational Masking in Humans
Authors: Yang Xiaoqin , Liu Lei , Yang Pengcheng , Ding Yu , Wang Changming , Li Liang
Published: May 2022

Prepulse inhibition (PPI) is the reduction in the acoustic startle reflex (ASR) when the startling stimulus (pulse) is preceded by a weaker, non-starting stimulus. This can be enhanced by facilitating selective attention to the prepulse against a noise-masking background. On the other hand, the facilitation of selective attention to a target speech can release the target speech from masking, particularly from speech informational masking. It is not clear whether attentional regulation also affects PPI in this kind of auditory masking. This study used a speech syllable as the prepulse to examine whether the masker type and perceptual spatial attention can affect the PPI or the scalp EEG responses to the prepulse in healthy younger-adult humans, and whether the ERPs evoked by the prepulse can predict the PPI intensity of the ASR. The results showed that the speech masker produced a larger masking effect than the noise masker, and the perceptual spatial separation facilitated selective attention to the prepulse, enhancing both the N1 component of the prepulse syllable and the PPI of the ASR, particularly when the masker was speech. In addition, there was no significant correlation between the PPI and ERPs under any of the conditions, but the perceptual separation-induced PPI enhancement and ERP N1P2 peak-to-peak amplitude enhancement were correlated under the speech-masking condition. Thus, the attention-mediated PPI is useful for differentiating noise energetic masking and speech informational masking, and the perceptual separation-induced release of the prepulse from informational masking is more associated with attention-mediated early cortical unmasking processing than with energetic masking. However, the processes for the PPI of the ASR and the cortical responses to the prepulse are mediated by different neural mechanisms.