bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Promising RILs for High Grain Zinc Through Genotype × Environment Analysis and Stable Grain Zinc QTL Using SSRs and SNPs in Rice (Oryza sativa L.)
Authors: Suman K. , Neeraja C. N. , Madhubabu P. , Rathod Santosha , Bej Sonali , Jadhav K. P. , Kumar J. Aravind , Chaitanya U. , Pawar Smita C. , Rani Surekha H. , Subbarao Lella V. , Voleti Sitapati R.
Published: February 2021

Polished rice is one of the commonly consumed staple foods across the world. However, it contains limited nutrients especially iron (Fe) and zinc (Zn). To identify promising recombinant inbred lines (RILs) for grain Zn and single plant yield, 190 RILs developed from PR116 and Ranbir Basmati were evaluated in two environments (E1 and E2). A subset of 44 contrasting RILs for grain Zn was screened in another two environments (E3 and E4). Phenotypic data was collected for 10 traits, viz., days to 50% flowering, plant height, panicle length, number of tillers, single plant yield (SPY), test weight, Fe and Zn in brown (IBR, ZBR), and polished rice (IPR, ZPR). Stepwise regression analysis of trait data in 190 RILs and a subset of 44 RILs revealed the interdependence of ZPR, ZBR, IPR, and IBR and the negative association of grain Zn with single plant yield. Based on the additive main effect and multiplicative interaction (AMMI) and genotype and genotype × environment interaction (GGE) analyses of the subset of 44 RILs across four environments (E1–E4), six promising RILs were identified for ZPR with >28 ppm. Mapping of 190 RILs with 102 simple sequence repeats (SSRs) resulted in 13 QTLs for best linear unbiased estimates (BLUEs) of traits including advantage over check (AOC). Using genotype-based sequencing (GBS), the subset of 44 RILs was mapped with 1035 single-nucleotide polymorphisms (SNPs) and 21 QTLs were identified. More than 100 epistatic interactions were observed. A major QTLqZPR.1.1(PV 37.84%) and another QTLqZPR.11.1(PV 15.47%) were identified for grain Zn in polished rice. A common major QTL (qZBR.2.1andqZPR.2.1) was also identified on chromosome 2 for grain Zn content across SSR and SNP maps. Two potential candidate genes related to transporters were identified based on network analyses in the genomic regions of QTL < 3 Mb. The RILs identified for grain Zn and SPY were nominated for national evaluation as under rice biofortification, and two QTLs identified based on BLUEs could be used in the rice biofortification breeding programs.
On Change of Soil Moisture Distribution With Vegetation Reconstruction in Mu Us Sandy Land of China, With Newly Designed Lysimeter
Authors: Cheng Yiben , Yang Wenbing , Zhan Hongbin , Jiang Qunou , Shi Mingchang , Wang Yunqi , Li Xinle , Xin Zhiming
Published: February 2021

Background

China’s so-called Three North Shelterbelt Program (3NSP) has produced a vast area of lined forest reconstruction in the semi-arid regions. This study uses the lined rain-fed Pinus sylvestris var. mongolica (PSM) sand-fixing forest in the eastern part of Mu Us Sandy Land in Northwestern China as an example to investigate the ecohydrological process in this region. Rain gauges, newly designed lysimeters and soil moisture sensors are used to monitor precipitation, deep soil recharge (DSR) and soil water content, where DSR specifically refers to recharge that can reach a depth more than 200 cm and eventually replenish the underneath groundwater reservoir.

Results

This study shows that there are two obvious moisture recharge processes in an annual base for the PSM forest soil: a snowmelt-related recharge process in the spring and a precipitation-related recharge process in the summer. The recharge depth of the first process can reach 180 cm without DSR occurring (in 2018). The second process results in noticeable DSR in 2018. Specifically, the DSR values over 2016–2018 are 1, 0.2, and 1.2 mm, respectively. To reach the recharge depths of 20, 40, 80, 120, 160, and 200 cm, the required precipitation intensities have to be 2.6, 3.2, 3.4, 8.2, 8.2, and 13.2 mm/d, respectively. The annual evapotranspiration in the PSM forest is 466.94 mm in 2016, 324.60 mm in 2017, and 183.85 mm in 2018.

Conclusion

This study concludes that under the current precipitation conditions (including both dry- and wet-years such as 2016–2018), water consumption of PSM somewhat equals to the precipitation amount, and PSM has evolved over years to regulate its evapotranspiration in response to annual precipitation fluctuations in Mu Us Sandy Land of China.
Biochemical Characterization and Differential Expression of PAL Genes Associated With “Translocated” Peach/Plum Graft-Incompatibility
Authors: Amri Rihab , Font i Forcada Carolina , Giménez Rosa , Pina Ana , Moreno María Ángeles
Published: February 2021

Grafting is an ancient plant propagation technique widely used in horticultural crops, particularly in fruit trees. However, the involvement of two different species in grafting may lead to lack of affinity and severe disorders between the graft components, known as graft-incompatibility. This complex agronomic trait is traditionally classified into two categories: “localized” (weak graft unions with breaks in cambial and vascular continuity at the graft interface and absence of visual symptoms in scion leaves and shoots) and “translocated” (degeneration of the sieve tubes and phloem companion cells at the graft interface causing translocation problems in neighboring tissues, and reddening/yellowing of scion leaves). Over the decades, more attention has been given to the different mechanisms underlying the “localized” type of graft-incompatibility; whereas the phenylpropanoid-derived compounds and the differential gene expression associated with the “translocated” graft-incompatibility remain unstudied. Therefore, the aim of this study was to shed light on the biochemical and molecular mechanisms involved in the typical “translocated” graft-incompatibility of peach/plum graft-combinations. In this study, the “Summergrand” (SG) nectarine cultivar was budded on two plum rootstocks: “Adara” and “Damas GF 1869”. “Translocated” symptoms of incompatibility were shown and biochemically characterized in the case of “SG/Damas GF 1869” graft-combination, 3 years after grafting. Non-structural carbohydrates (soluble sugars and starch), phenolic compounds and antioxidant activity, were significantly enhanced in the incompatible graft-combination scion. Similarly, the enzymatic activities of the antioxidant enzyme peroxidase, the phenylalanine ammonia-lyase (PAL) and polyphenol oxidase involved in the phenylpropanoid pathway were significantly affected by the incompatible rootstock “Damas GF 1869”, inducing higher activities in the scion than those induced by the compatible rootstock “Adara”. In addition, a positive and strong correlation was obtained between total phenol content, antioxidant capacity and the expression of the key genes involved in the phenylpropanoid pathway,PAL1andPAL2. Regarding the “SG/Adara” graft-combination, there were neither external symptoms of “translocated” incompatibility nor significant differences in the biochemical and molecular parameters between scion and rootstock, proving it to be a compatible combination. The differential expression ofPALgenes together with the biochemical factors cited above could be good markers for the “translocated” peach/plum graft-incompatibility.
Silicon-Mediated Priming Induces Acclimation to Mild Water-Deficit Stress by Altering Physio-Biochemical Attributes in Wheat Plants
Authors: Hameed Arruje , Farooq Tahir , Hameed Amjad , Sheikh Munir Ahmad
Published: February 2021

Water-deficit stress negatively affects seed germination, seedling development, and plant growth by disrupting cellular and metabolic functions, reducing the productivity and yield of field crops. In this study, sodium silicate (SS) has been employed as a seed priming agent for acclimation to mild water-deficit stress by invoking priming memory in wheat plants. In pot experiments, the SS-primed (20, 40, and 60 mM) and non-primed control seeds were allowed to grow under normal and mild water-deficit conditions. Subsequently, known methods were followed for physiological and biochemical studies using flag leaves of 98-day mature wheat plants. The antioxidant and hydrolytic enzymes were upregulated, while proteins, reducing sugars, total sugars, and glycine betaine increased significantly in the flag leaves of wheat plants originated from SS-treated seeds compared to the control under mild water-deficit stress. Significant decreases in the malondialdehyde (MDA) and proline contents suggested a controlled production of reactive oxygen species, which resulted in enhanced cell membrane stability. The SS priming induced a significant enhancement in yield, plant biomass, and 100-grain weight of wheat plants under water-deficit stress. The improvement in the yield parameters indicated the induction of Si-mediated stress acclimation in SS-primed seeds that elicited water-deficit tolerance until the maturity of plants, ensuring sustainable productivity of climate-smart plants.
Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development
Authors: Li Lin , Liu Yuanchu , Huang Ying , Li Bin , Ma Wen , Wang Donghao , Cao Xiaoyan , Wang Zhezhi
Published: February 2021

Salvia miltiorrhiza Bunge (S. miltiorrhiza), a traditional Chinese medicinal herb, contains numerous bioactive components with broad range of pharmacological properties. By increasing the levels of endogenous jasmonate (JA) in plants or treating them with methyl jasmonate (MeJA), the level of tanshinones and salvianolic acids can be greatly enhanced. The jasmonate ZIM (JAZ) proteins belong to the TIFY family, and act as repressors, releasing targeted transcriptional factors in the JA signaling pathway. Herein, we identified and characterized 15 TIFY proteins present in S. miltiorrhiza. Quantitative reverse transcription PCR analysis indicated that the JAZ genes were all constitutively expressed in different tissues and were induced by MeJA treatments. SmJAZ3, which negatively regulates the tanshinones biosynthesis pathway in S. miltiorrhiza and the detailed molecular mechanism is poorly understood. SmJAZ3 acts as a bait protein to capture and identify a WD-repeat containing the protein SmWD40-170. Further molecular and genetic analysis revealed that SmWD40-170 is a positive regulator, promoting the accumulation of secondary metabolites in S. miltiorrhiza. Our study systematically analyzed the TIFY family and speculated a module of the JAZ-WD40 complex provides new insights into the mechanisms regulating the biosynthesis of secondary metabolites in S. miltiorrhiza.
Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species
Authors: Pan Meng , Zhu Haiyan , Tian Chengming , Huang Manrong , Fan Xinlei
Published: February 2021

Cytospora species are widely distributed and often occur as endophytes, saprobes or phytopathogens. They primarily cause canker and dieback diseases of woody host plants, leading to the growth weakness or death of host plants, thereby causing significant economic and ecological losses. In order to reveal the diversity of Cytospora species associated with canker and dieback diseases of coniferous trees in China, we assessed 11 Cytospora spp. represented by 28 fungal strains from symptomatic branches or twigs of coniferous trees, i.e., Juniperus procumbens, J. przewalskii, Picea crassifolia, Pinus armandii, P. bungeana, Platycladus orientalis in China. Through morphological observations and multilocus phylogeny of ITS, LSU, act, rpb2, tef1-α, and tub2 gene sequences, we focused on four novel Cytospora species (C. albodisca, C. discostoma, C. donglingensis, and C. verrucosa) associated with Platycladus orientalis. This study represented the first attempt to clarify the taxonomy of Cytospora species associated with canker and dieback symptoms of coniferous trees in China.
Genetic Control Diversity Drives Differences Between Cadmium Distribution and Tolerance in Rice
Authors: Chen Yi-Bo , Chen Yu-Chao , Zhu Yu-Xing , Li Sai , Deng Hua-bing , Wang Jiu-Rong , Tang Wen-Bang , Sun Liang
Published: February 2021

Rice, a staple crop for nearly half the planet’s population, tends to absorb and accumulate excessive cadmium (Cd) when grown in Cd-contaminated fields. Low levels of Cd can degrade the quality of rice grains, while high levels can inhibit the growth of rice plants. There is genotypic diversity in Cd distribution and Cd tolerance in different rice varieties, but their underlying genetic mechanisms are far from elucidated, which hinders genetic improvements. In this study, a joint study of phenotypic investigation with quantitative trait loci (QTLs) analyses of genetic patterns of Cd distribution and Cd tolerance was performed using a biparent population derived from japonica and indica rice varieties. We identified multiple QTLs for each trait and revealed that additive effects from various loci drive the inheritance of Cd distribution, while epistatic effects between various loci contribute to differences in Cd tolerance. One pleiotropic locus, qCddis8, was found to affect the Cd distribution from both roots to shoots and from leaf sheaths to leaf blades. The results expand our understanding of the diversity of genetic control over Cd distribution and Cd tolerance in rice. The findings provide information on potential QTLs for genetic improvement of Cd distribution in rice varieties.
Structural Characteristics of the Guaiacyl-Rich Lignins From Rice (Oryza sativa L.) Husks and Straw
Authors: Rosado Mario J. , Rencoret Jorge , Marques Gisela , Gutiérrez Ana , del Río José C.
Published: February 2021

Rice (Oryza sativa L.) is a major cereal crop used for human nutrition worldwide. Harvesting and processing of rice generates huge amounts of lignocellulosic by-products such as rice husks and straw, which present important lignin contents that can be used to produce chemicals and materials. In this work, the structural characteristics of the lignins from rice husks and straw have been studied in detail. For this, whole cell walls of rice husks and straw and their isolated lignin preparations were thoroughly analyzed by an array of analytical techniques, including pyrolysis coupled to gas chromatography-mass spectrometry (Py-GC/MS), nuclear magnetic resonance (NMR), and derivatization followed by reductive cleavage (DFRC). The analyses revealed that both lignins, particularly the lignin from rice husks, were highly enriched in guaiacyl (G) units, and depleted in p-hydroxyphenyl (H) and syringyl (S) units, with H:G:S compositions of 7:81:12 (for rice husks) and 5:71:24 (for rice straw). These compositions were reflected in the relative abundances of the different interunit linkages. Hence, the lignin from rice husks were depleted in β–O–4′ alkyl-aryl ether units (representing 65% of all inter-unit linkages), but presented important amounts of β–5′ (phenylcoumarans, 23%) and other condensed units. On the other hand, the lignin from rice straw presented higher levels of β–O–4′ alkyl-aryl ethers (78%) but lower levels of phenylcoumarans (β–5′, 12%) and other condensed linkages, consistent with a lignin with a slightly higher S/G ratio. In addition, both lignins were partially acylated at the γ-OH of the side-chain (ca. 10–12% acylation degree) with p-coumarates, which overwhelmingly occurred over S-units. Finally, important amounts of the flavone tricin were also found incorporated into these lignins, being particularly abundant in the lignin of rice straw.
Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability
Authors: McKenna Joseph F. , Gumber Hardeep K. , Turpin Zachary M. , Jalovec Alexis M. , Kartick Andre C. , Graumann Katja , Bass Hank W.
Published: February 2021

In eukaryotes, the nuclear envelope (NE) encloses chromatin and separates it from the rest of the cell. The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex physically bridges across the NE, linking nuclear and cytoplasmic components. In plants, these LINC complexes are beginning to be ascribed roles in cellular and nuclear functions, including chromatin organization, regulation of nuclei shape and movement, and cell division. Homologs of core LINC components, KASH and SUN proteins, have previously been identified in maize. Here, we characterized the presumed LINC-associated maize nucleoskeletal proteins NCH1 and NCH2, homologous to members of the plant NMCP/CRWN family, and MKAKU41, homologous to AtKAKU4. All three proteins localized to the nuclear periphery when transiently and heterologously expressed as fluorescent protein fusions in Nicotiana benthamiana. Overexpression of MKAKU41 caused dramatic changes in the organization of the nuclear periphery, including nuclear invaginations that stained positive for non-nucleoplasmic markers of the inner and outer NE membranes, and the ER. The severity of these invaginations was altered by changes in LINC connections and the actin cytoskeleton. In maize, MKAKU41 appeared to share genetic functions with other LINC components, including control of nuclei shape, stomatal complex development, and pollen viability. Overall, our data show that NCH1, NCH2, and MKAKU41 have characteristic properties of LINC-associated plant nucleoskeletal proteins, including interactions with NE components suggestive of functions at the nuclear periphery that impact the overall nuclear architecture.
Stressful Life Events and Subjective Well-Being in Vocational School Female Adolescents: The Mediating Role of Depression and the Moderating Role of Perceived Social Support
Authors: Ouyang Mingkun , Gui Danni , Cai Xiao , Yin Yulong , Mao Xiaoling , Huang Shaoxu , Zeng Pan , Wang Pengcheng
Published: February 2021

Stressful life events and subjective well-being are negatively related, but there is little research in the current literature exploring the mediating and moderating mechanisms underlying this association, especially for female adolescents in vocational schools who are subjected to undesirable life events. In the present study, we examined the mediating role of depression in the association between stressful life events and female adolescents’ subjective well-being, as well as the moderating role of perceived social support in the direct and indirect relations involved. The participants were 1,096 vocational school female adolescents, who completed the questionnaires regarding stressful life events, subjective well-being, depression, and perceived social support. The results showed that depression partially mediated the relation between stressful life events and subjective well-being. Importantly, perceived social support moderated the direct link between stressful life events and subjective well-being, and the indirect link between stressful life events and depression, but not the indirect link between depression and subjective well-being. Especially, female adolescents high in perceived social support displayed higher levels of subjective well-being and lower levels of depression in facing with stressful life events than those low in perceived social support. These findings highlight the mechanisms underlying the relationship between stressful life events and subjective well-being in vocational school female adolescents.
How Does Self-Concept Differentiation Work in Chinese Retirees: A Moderated Mediation Analysis
Authors: Zhu Changzheng , Zhu Min , Gao Xiangping , Liu Xiaoshi
Published: February 2021

Self-concept differentiation (SCD) is a sign of fragmentation of the self rather than specialization of role identities for its robust relationship with psychological adjustment. However, little is known about the mechanisms underlying the relationship between SCD and psychological adjustment. The aim of this study was to examine the mediating role of self-consistency and congruence (SCC) in the association between SCD and psychological adjustment (psychological well-being, depression, and anxiety), and the moderating role of age in the relationship between SCD and SCC. This moderated mediation model was examined among 158 Chinese retirees (mean age = 71.12, SD = 9.13), who completed measurements regarding SCD, SCC, psychological well-being, anxiety, and depression. The results showed that SCC partially mediated the links between SCD and the indices of psychological adjustment. Furthermore, age moderated this mediation effect, which was found in mean and high-age participants, but not in low-age ones. Our findings indicate that, at different age stages, the internal mechanisms of SCD affecting psychological adaptation are not the same, and a low differentiated or highly integrated self can serve as an adaptive resource to maintain high subjective well-being of the elderly and protect them from anxiety and depression.
Drawing on Dialogues in Arts-Based Dynamic Interpersonal Therapy (ADIT) for Complex Depression: A Complex Intervention Development Study Using the Medical Research Council (UK) Phased Guidance
Authors: Havsteen-Franklin Dominik , Oley Mary , Sellors Sarah Jane , Eagles Diane
Published: February 2021

Aim: The aim of this paper is to present the development and evaluation of an art psychotherapy brief treatment method for complex depression for patients referred to mental health services.

Background: Art Psychotherapy literature describes a range of processes of relational change through the use of arts focused and relationship focused interventions. Complex depression has a prevalence of 3% of the population in the West and it is recorded that in 2016 only 28% of that population were receiving psychological treatment. This study was developed to test the hypothesis of whether an accessible and acceptable approach to the treatment of complex depression could be developed in relation to existing evidence-based practice within mental health services.

Method: The United Kingdom Medical Research Council phased guidance for complex intervention development was used (Phases I and II) to develop the intervention. The process included producing a literature overview, systematic description of clinical practice, including a logic model and a clinical protocol. The art psychotherapy protocol described an arts-based dynamic interpersonal therapy approach (ADIT), offered 1:1 over 24 sessions. Further to this the intervention was tested for referrer acceptability. The intervention is in the early stages of evaluation, using changes to the patient's depression and anxiety measured pre- and post-treatment with a follow-up measure at 3 months following completion of treatment.

Results: Phase I of the study provided a good basis for developing a logic model and protocol. The authors found that there was good clinical consensus about the use of a structured clinical art psychotherapy method (ADIT) and the literature overview was used to support specific examples of good practice. The verification of clinical coherence was represented by a logic model and clinical protocol for delivering the intervention. The acceptability study demonstrated very high levels of acceptability for referrers reporting that (i) ADIT was acceptable for patients with complex/major depression, (ii) that they were likely to refer to ADIT in the future (iii) that the use of arts was likely to improve accessibility (iv) the use of arts was likely to improve outcomes and (v) that offering ADIT was an effective use of mental health resources.

Discussion: Phase I of this intervention development study (following MRC guidance) demonstrated theoretical and practice coherence resulting in a clinical protocol and logic model. Whilst Phase II of this study showed promising results, Phase II would need to be sufficiently scaled up to a full trial to further test the intervention and protocol.
Videoconferencing Psychotherapy During the Pandemic: Exceptional Times With Enduring Effects?
Authors: Fernández-Álvarez Javier , Fernández-Álvarez Héctor
Published: February 2021

With the advent of COVID-19, a sudden, unexpected, and forced shift has been produced in the field of psychotherapy. Worldwide, many therapists closed their offices and started to deliver psychotherapy online through a screen. Although different media started to be incorporated, videoconferencing is undoubtedly the most common way in which therapists are doing therapy these days. This is catalyzing a rapid change in the practice of psychotherapy with probable lasting effects and deserves to be carefully reflected upon. Therefore, in this paper our aim is to outline the main challenges for a medium that may have arrived to stay. In that sense, we review the literature to describe the state-of-the-art regarding the main aspects of videoconferencing psychotherapy as well as to suggest possible avenues for future research and practice.
The Effects of an In-vehicle Collision Warning System on Older Drivers' On-road Head Movements at Intersections
Authors: Shichrur Rachel , Ratzon Navah Z. , Shoham Arava , Borowsky Avinoam
Published: February 2021

With age might come a decline in crucial driving skills. The effect of a collision warning system (CWS) on older drivers' head movements behavior at intersections was examined.

Methods: Twenty-six old-adults, between 55 and 64 years of age, and 16 Older drivers between 65 and 83 years of age, participated in the study. A CWS (Mobileye Inc.) and a front-back in-vehicle camera (IVC) were installed in each of the participants' own vehicles for 6 months. The CWS was utilized to identify unsafe events during naturalistic driving situations, and the IVC was used to capture head direction at intersections. The experimental design was conducted in three phases (baseline, intervention, and carryover), 2 months each. Unsafe events were recorded by the CWS during all phases of the study. In the second phase, the CWS feedback was activated to examine its effect on drivers' head movement' behavior at intersections.

Results: Older drivers (65+) drove significantly more hours in total during the intervention phase (M = 79.1 h, SE = 10) than the baseline phase (M = 39.1 h, SE = 5.3) and the carryover phase (M = 37.7 h, SE = 5.4). The study revealed no significant differences between the head movements of older and old-adult drivers at intersections. For intersection on the left direction, a significant improvement in drivers' head movements' behavior was found at T-junctions, turns and four-way intersections from phase 1 to phase 3 (p < 0.01), however, two intersection types presented a decrease along the study phases. The head movements' behavior at roundabouts and merges was better at phase 1 compared to phase 3 (p < 0.01). There was no significant reduction of the mean number of CWS unsafe events across the study phases.

Conclusions: The immediate feedback provided by the CWS was effective in terms of participants' head movements at certain intersections but was harmful in others. However, older drivers drove many more hours during the active feedback phase, implying that they trusted the system. Therefore, in the light of this complex picture, using the technological feedback with older drivers should be followed with an additional mediation or follow-up to ensure safety.
Efforts on Changing Lifestyle Behaviors May Not Be Enough to Improve Health-Related Quality of Life Among Adolescents: A Cluster-Randomized Controlled Trial
Authors: Bandeira Alexsandra da Silva , Beets Michael W. , Silveira Pablo Magno da , Lopes Marcus Vinicius Veber , Barbosa Filho Valter Cordeiro , da Costa Bruno G. G. , Silva Kelly Samara
Published: February 2021

Schools have been the main context for physical activity (PA) and sedentary behavior (SB) interventions among adolescents, but there is inconsistent evidence on whether they also improve dimensions of the health−related quality of life (HRQoL). The aim of this study was to evaluate the effects of a school-based active lifestyle intervention on dimensions of HRQoL. A secondary aim was to verify whether sex, age, and HRQoL at baseline were moderators of the intervention effect. A cluster-randomized controlled trial was conducted at three control and three intervention schools in Florianopolis, Brazil. All students from 7th to 9th grade were invited to participate. A school year intervention, designed primarily to increase PA and reduce SB, included strategies focused on (i) teacher training on PA, SB, and nutrition, and availability of teaching materials related to these contents; (ii) environmental improvements (i.e., creation and revitalization of spaces for the practice of PA in school); and (iii) education strategies, with the availability of folders and posters regarding PA, SB, and nutrition. Participants and the research staffs were not blinded to group assignment, but a standardized evaluation protocol was applied at baseline and after the intervention (March and November 2017) using the KIDSCREEN−27 to assess HRQoL across five dimensions. Mixed linear models were performed to evaluate the effect of the Movimente intervention on the five HRQoL dimensions. Of the 921 students who answered the questionnaire at baseline, 300 and 434 completed the study in control and intervention groups, respectively (dropouts: 20%). The results revealed no significant effects of the intervention on any HRQoL dimensions. A reduction of the school environment dimension was observed in both the control (-2.44; 95% CI: -3.41 to -1.48) and intervention groups (-2.09; 95% CI: -2.89 to -1.30). Sensitivity analyses showed that students in the highest baseline tertiles of HRQoL in any dimension had a reduction in their respective scores from pre- to post-intervention in both school groups. In conclusion, our results demonstrated no intervention effect on HRQoL dimensions and those students with the highest levels of HRQoL at baseline on all dimensions reduced from pre to post-intervention.

Clinical Trial Registration

The trial is registered at the Clinical Trial Registry (Trial ID: NCT02944318; date of registration: October 18, 2016).
Habits and Psychological Factors Associated With Changes in Physical Activity Due to COVID-19 Confinement
Authors: León-Zarceño Eva , Moreno-Tenas Antonio , Boix Vilella Salvador , García-Naveira Alejo , Serrano-Rosa Miguel Angel
Published: February 2021

The confinement that COVID-19 has brought about has had a negative influence on people’s psychological health. However, this impact is not widespread throughout the population, and men and women may be affected differently and it is not known what protective factors may exist. In this sense, physical activity has classically been shown to be a habit associated with psychological health. The study aimed to analyze the impact of confinement on psychological health (psychological well-being, coping, emotions, and perception of daily difficulties), taking into account gender, and perceived changes in physical activity. After the project was approved by the University’s Ethics Commission, the participants, after signing the informed consent, completed the online questionnaires during the days from 6 to 20 April, the time when, in Spain, confinement was in place and the highest peak of deaths and infections from COVID-19 occurred. A total of 457 Spanish participants (247 men and 210 women) were evaluated in psychological well-being, in its adaptation to Spanish, in coping, with the Spanish adaptation of the COPE Inventory, in daily habits and difficulties (ad hoc questionnaire) and the level of physical activity they had (sedentary, active, and federated players) was recorded. Besides, the perceived change in physical activity due to confinement was recorded. The results showed that perceived emotions, difficulties for certain routines, psychological well-being, and coping differed according to sex. On the other hand, depending on the change in physical activity habits, it was observed that participants who increased their physical activity responded differently in the perception of emotions, and difficulties for routines and in psychological well-being. Finally, differences were also observed in most variables between sedentary, active, and federated participants. Results are discussed highlighting the importance of physical activity as a moderating factor of the impact of confinement.
Understanding the Multidimensional and Dynamic Nature of Facial Expressions Based on Indicators for Appraisal Components as Basis for Measuring Drivers' Fear
Authors: Zhang Meng , Ihme Klas , Drewitz Uwe , Jipp Meike
Published: February 2021

Facial expressions are one of the commonly used implicit measurements for the in-vehicle affective computing. However, the time courses and the underlying mechanism of facial expressions so far have been barely focused on. According to the Component Process Model of emotions, facial expressions are the result of an individual's appraisals, which are supposed to happen in sequence. Therefore, a multidimensional and dynamic analysis of drivers' fear by using facial expression data could profit from a consideration of these appraisals. A driving simulator experiment with 37 participants was conducted, in which fear and relaxation were induced. It was found that the facial expression indicators of high novelty and low power appraisals were significantly activated after a fear event (high novelty: Z = 2.80, p < 0.01, rcontrast = 0.46; low power: Z = 2.43, p < 0.05, rcontrast = 0.50). Furthermore, after the fear event, the activation of high novelty occurred earlier than low power. These results suggest that multidimensional analysis of facial expression is suitable as an approach for the in-vehicle measurement of the drivers' emotions. Furthermore, a dynamic analysis of drivers' facial expressions considering of effects of appraisal components can add valuable information for the in-vehicle assessment of emotions.
Percentiles and Principal Component Analysis of Physical Fitness From a Big Sample of Children and Adolescents Aged 6-18 Years: The DAFIS Project
Authors: Iglesias-Soler Eliseo , Rúa-Alonso María , Rial-Vázquez Jessica , Lete-Lasa Jose Ramón , Clavel Iván , Giráldez-García Manuel A. , Rico-Díaz Javier , Corral Miguel Rodríguez-Del , Carballeira-Fernández Eduardo , Dopico-Calvo Xurxo
Published: February 2021

Assessing physical fitness has emerged as a proxy of the health status of children and adolescents and therefore as relevant from a public health point of view. DAFIS is a project included in Plan Galicia Saudable (Healthy Galicia Plan) of the regional government of Galicia (Spain). DAFIS consists of an on-line software devoted to record the results of a standard physical fitness protocol carried out as a part of the physical education curriculum. The aims of this study were: to obtain normative values of physical fitness of the Galician school population evaluated in the DAFIS project, and to identify a reduced number of components and tests able to capture a significant amount of the variability in the physical fitness of children and adolescents. From an initial sample of 27784 records, 15287 cases (7543 males, 7744 females) were considered after filtering. Generalized Additive Models for Location, Scale and Shape were used for obtaining percentile curves and tables for each sex. Furthermore, a principal components analysis was performed, selecting the number of components by applying the Kaiser’s rule and selecting a subset of variables considering the correlation between each variable and the components. Percentile curves and normative values are reported for each test and sex. Physical fitness was better in boys than in girls throughout age groups, except for flexibility that was consistently higher in girls. Two main components were detected throughout age groups: the first one representing body composition and partially cardiorespiratory fitness and the second one muscular fitness. For boys and girls, waist to height ratio had the highest correlations with the first component in four out of six age groups. The highest correlation with the second component, was most frequently observed for the handgrip test both in boys and girls (four out of six age groups). This study provides evidence about the utility of school community actions like DAFIS aimed to track the health-related fitness of children and adolescents. The results suggest that fat mass distribution (i.e., waist to height ratio and waist circumference) and muscular performance (mainly handgrip) concentrate a high proportion physical fitness variance.
Controlling Video Stimuli in Sign Language and Gesture Research: The OpenPoseR Package for Analyzing OpenPose Motion-Tracking Data in R
Authors: Trettenbrein Patrick C. , Zaccarella Emiliano
Published: February 2021

Researchers in the fields of sign language and gesture studies frequently present their participants with video stimuli showing actors performing linguistic signs or co-speech gestures. Up to now, such video stimuli have been mostly controlled only for some of the technical aspects of the video material (e.g., duration of clips, encoding, framerate, etc.), leaving open the possibility that systematic differences in video stimulus materials may be concealed in the actual motion properties of the actor’s movements. Computer vision methods such as OpenPose enable the fitting of body-pose models to the consecutive frames of a video clip and thereby make it possible to recover the movements performed by the actor in a particular video clip without the use of a point-based or markerless motion-tracking system during recording. The OpenPoseR package provides a straightforward and reproducible way of working with these body-pose model data extracted from video clips using OpenPose, allowing researchers in the fields of sign language and gesture studies to quantify the amount of motion (velocity and acceleration) pertaining only to the movements performed by the actor in a video clip. These quantitative measures can be used for controlling differences in the movements of an actor in stimulus video clips or, for example, between different conditions of an experiment. In addition, the package also provides a set of functions for generating plots for data visualization, as well as an easy-to-use way of automatically extracting metadata (e.g., duration, framerate, etc.) from large sets of video files.
The Measurement of Individual Differences in Cognitive Biases: A Review and Improvement
Authors: Berthet Vincent
Published: February 2021

Individual differences have been neglected in decision-making research on heuristics and cognitive biases. Addressing that issue requires having reliable measures. The author first reviewed the research on the measurement of individual differences in cognitive biases. While reliable measures of a dozen biases are currently available, our review revealed that some measures require improvement and measures of other key biases are still lacking (e.g., confirmation bias). We then conducted empirical work showing that adjustments produced a significant improvement of some measures and that confirmation bias can be reliably measured. Overall, our review and findings highlight that the measurement of individual differences in cognitive biases is still in its infancy. In particular, we suggest that contextualized (in addition to generic) measures need to be improved or developed.
Embodied Displays of “Doing Thinking.” Epistemic and Interactive Functions of Thinking Displays in Children's Argumentative Activities
Authors: Heller Vivien
Published: February 2021

This study investigates moments in which one participant in an interaction embodies that he is “doing thinking,” a display that is commonly referred to as “thinking face. ” From an interactional perspective, it is assumed that embodied displays of “doing thinking” are a recurring social practice and serve interactive functions. While previous studies have examined thinking faces primarily in word searches and storytelling, the present study focuses on argumentative activities, in which children engage in processes of joint decision-making. The paper has two interrelated aims. The first aim is to describe how multiple modalities—beyond the face—are temporally coordinated to create multimodal gestalts of “doing thinking.” It is shown that thinking displays not only involve dynamic imaginative gaze but also stylized bodily postures. The second aim is to generate knowledge about the functions of thinking displays in children's argumentative activities. The analysis describes how both speakers and recipients use thinking displays in different turn positions and align them with verbal talk or silence. The data for this study comprise video recordings of decision-making processes in groups of older children. Drawing on a multimodal approach to situated interaction, it will be proposed that embodied displays of “doing thinking” provide a resource to shape participation frameworks, mark epistemic stances and create epistemic ecologies for collaborative reasoning. By investigating thinking displays in a particular conversational activity, the study sheds light on the diversity and context-sensitive functionality of thinking displays. It also contributes to recent research on children's collaborative reasoning as an embodied discursive practice.
Variability of Practice, Information Processing, and Decision Making—How Much Do We Know?
Authors: Czyż Stanisław H.
Published: February 2021

Decision-making is a complex action requiring efficient information processing. Specifically, in movement in which performance efficiency depends on reaction time, e.g., open-loop controlled movements, these processes may play a crucial role. Information processing includes three distinct stages, stimulus identification, response selection, and response programming. Mainly, response selection may play a substantial contribution to the reaction time and appropriate decision making. The duration of this stage depends on the number of possible choices an individual has to “screen” to make a proper decision. Given that reaction time is crucial in many sports, the possibilities of reducing it through practice are very tempting. The information processing and its relationship to the manner an individual is practicing are discussed. Especially the variability of practice issues will be explored. In variable practice conditions, an individual has to react to one or more stimuli and has to produce one of the many variations of the same movement or different movements they learned. One has to identify a stimulus appropriately and has to select a response optimally, i.e., choosing from as few choices as possible to reduce the reaction time. On the other hand, in constant practice conditions, an individual can be exposed to one or many stimuli. Still, there is only one variation of the movement that can be executed in the presence of a learned stimulus. Based on the information processing theory and the results of the research focusing on variability of practice, I discuss how the practice conditions may affect reaction time and, as a result, the decision-making process. I conceptually frame the possible implications of practice conditions on decision making related to information processing. In this review, a possible mechanism and relationship between practice conditions and decision-making are presented. Future research directions are presented.
Impairment in Emotional Intelligence May Be Mood-Dependent in Bipolar I and Bipolar II Disorders
Authors: Kuo Shih-Yu , Chang Yun-Hsuan , Wang Tzu-Yun , Tseng Huai-Hsuan , Huang Chih-Chun , Chen Po See , Lane Hsien-Yuan , Yang Yen Kuang , Lu Ru-Band
Published: February 2021

Background: An emotional intelligence (EI) deficit has been noticed in euthymic bipolar spectrum disorder (BD) patients. However, whether this deficit is affected by mood or subtype is unclear.

Objectives:The aim of this study was to investigate whether an EI deficit is mood-dependent, and which mood symptoms have more impact on EI in BD.

Methods: Two hundred and thirty participants aged between 18 and 65 years old were recruited [130 BD patients (51 bipolar I disorder (BDI) and 79 bipolar II disorder (BDII): 39.2% males; 91 healthy controls (HCs): 48.4% males)]. The Mayer–Salovey–Caruso Emotional Intelligence Test (MSCEIT), which contains experiential and strategic EI ratings, was used to assess social cognition. The Hamilton Depression Rating Scale (HDRS) and the Young's Mania Rating Scale (YMRS) were used for evaluating the severity [HAMD and YMRS scores ≦7 were euthymic (BDeut) and HAMD YMRS sores ≧8 were episodic (BDepi)]. Analyses of covariance (ANCOVA) were performed, with adjustment for background information between the BD patients and HCs.

Results: The results showed that, compared to the HCs, the BDeut patients showed no difference in any MSCEIT measures, while the BDepi patients showed lower scores in all MSCEIT measures, except for perceiving emotions. In addition, a main effect of mood state instead of BD subtype was found for the managing emotions branch (p < 0.0007). Regression analyses showed that the duration of illness and HDRS scores were correlated with the scores in the strategic area of the MSCEIT, while age and YMRS scores were more relevant to the scores in the experiential area of the MSCEIT.

Conclusion: The results confirm that an EI deficit is mood-dependent in BD patients. In addition, a depressive mood is more related to the strategic EI area, while a manic mood is correlated with the experiential EI area. Understanding the different domains of EI deficits in BD patients may be helpful for developing interventions for BD.
Intermittent Theta-Burst Stimulation Over the DorsoLateral PreFrontal Cortex (DLPFC) in Healthy Subjects Produces No Cumulative Effect on Cortical Excitability
Authors: Bouaziz Noomane , Laidi Charles , Thomas Fanny , Schenin-King Andrianisaina Palmyre , Moulier Virginie , Januel Dominique
Published: February 2021

Background: Intermittent Theta Burst Stimulation (iTBS) is a design of repetitive Transcranial Magnetic Stimulation (rTMS) and could be a candidate to replace rTMS in the treatment of depression, thanks to its efficacy, shorter duration, and ease of use. The antidepressant mechanism of iTBS, and whether this mechanism is mediated by a modulation of cortical excitability, remains unknown.

Methods: Using a randomized double-blind, sham-controlled trial, 30 healthy volunteers received either iTBS or a sham treatment targeting the left DorsoLateral PreFrontal Cortex (L-DLPFC), twice a day over 5 consecutive days. Cortical excitability was measured before and after the 5 days of stimulation.

Results: No difference in cortical excitability was observed between active or sham iTBS.

Conclusion: Our study does not support any effect on cortical excitability of repetitive iTBS targeting the L-DLPFC.