bluuebunny/crossref_metadata_2025 huggingface dataset:
Unusual Cell Structures and Organelles in Giardia intestinalis and Trichomonas vaginalis Are Potential Drug Targets
Authors: Benchimol Marlene , Gadelha Ana Paula , de Souza Wanderley
Published: November 2022

This review presents the main cell organelles and structures of two important protist parasites, Giardia intestinalis, and Trichomonas vaginalis; many are unusual and are not found in other eukaryotic cells, thus could be good candidates for new drug targets aimed at improvement of the chemotherapy of diseases caused by these eukaryotic protists. For example, in Giardia, the ventral disc is a specific structure to this parasite and is fundamental for the adhesion and pathogenicity to the host. In Trichomonas, the hydrogenosome, a double membrane-bounded organelle that produces ATP, also can be a good target. Other structures include mitosomes, ribosomes, and proteasomes. Metronidazole is the most frequent compound used to kill many anaerobic organisms, including Giardia and Trichomonas. It enters the cell by passive diffusion and needs to find a highly reductive environment to be reduced to the nitro radicals to be active. However, it provokes several side effects, and some strains present metronidazole resistance. Therefore, to improve the quality of the chemotherapy against parasitic protozoa is important to invest in the development of highly specific compounds that interfere with key steps of essential metabolic pathways or in the functional macromolecular complexes which are most often associated with cell structures and organelles.
Multivariate Analysis with XRD Data as a Fingerprinting Technique to Study Burned Soils
Authors: Rocha Débora R. , Barber Xavier , Jordán-Vidal Manuel M. , Urbano Alexandre , Melquiades Fábio L. , Thomaz Edivaldo L. , Mataix-Solera Jorge
Published: November 2022

Fire is a natural process with recognized recurrence. However, ongoing climate change and human activities are causing some disturbances in their natural regimes in most ecosystems. It is important to improve the methodologies used to evaluate the fire-induced changes in soils. This study aims at investigating the potential of the X-ray diffraction (XRD) technique to be used as a fingerprinting technique for burned soils. Multivariate analysis was employed to analyze the XRD data. Hierarchical Cluster Analysis (HCA) and local Partial Least Squares (PLS-2) models were performed. The soil samples are classified as Ferralsols and were collected from an Amazon region, Brazil, from forests, pastures and a slash-and-burn area. The studied temperatures ranged between 25 and 800 °C. Major differences were found for gibbsite, goethite and kaolinite contents due to dehydration. PLS-2 analysis presented better results than HCA as it provided information concerning the two features of the investigated soils, the collection site and the temperature. Therefore, it was possible to characterize soils from different sites and soils heated at different temperatures by using XRD data with multivariate analysis. Such methodology provided important information that may be used in areas with these environmental and soil conditions.
Magnetotactic Bacteria: From Evolution to Biomineralization and Biomedical Applications
Authors: Strbak Oliver , Hnilicova Petra , Gombos Jan , Lokajova Alica , Kopcansky Peter
Published: November 2022

The synthesis of magnetosomes in magnetotactic bacteria (MTB) represents probably one of Earth’s most ancient forms of biomineralization. The evolution of magnetosomes and the origin of magnetotaxis date back to the Archean Eon, 4.4–2.5 Ga ago. Magnetosomes consist of fine magnetite nanocrystals coated with a lipidic envelope. Their findings in eukaryotic cells and animals support the evolutionary success of otherwise energetically very demanding biocrystallization. Moreover, the conservation of magnetite biomineralization genes in all domains of life has been proposed very recently. Therefore, it is not surprising that magnetosomes have attracted attention from various scientific fields, including mineralogy, microbiology, biochemistry, biophysics, and bioengineering. Here, we review the most recent iron flow findings that lead to magnetite nanocrystals’ biomineralization in MTB. We emphasize the historical milestones that formed the evolution of magnetosomes and magnetotaxis functionality. Finally, we discuss the usability of these unique structures in biomedical, biotechnological, environmental, and nutritional applications.
Three-Dimensional Fault-Fold Growth Deciphered from Combined Seismic and Geological Data: A Case Study from the Xiongpo Anticline, Longmen Shan Piedmont
Authors: Li Xianyi , Zheng Xinru , Dai Xiangming , Almeida Rafael , Sun Chuang
Published: November 2022

The Xiongpo fault-fold belt shows prominent NE, ENE- and ~N–S-trending relief, which resulted from multi-stage upper crustal shortening in the Longmen Shan piedmont during the eastward growth of the eastern Tibetan Plateau. Previous studies have determined its 2D structural configurations from seismic profiles and field-based geological cross-sections. Here, we extend this analysis into the entire belt to explore the 3D structural evolution of this complex fault-fold belt and have built a 3D regional fault model. The results reveal along-strike variation of subsurface structural architecture of the Xiongpo fault-fold belt, which is characterized by transformation from a complex superimposition of a deep fault-bend fold beneath a shallow structural wedge in the center segment to a simple shallow fault-bend fold on both ends of the structure, and then to a trishear fault propagation fold on the plunging edges. This structural transformation determines the contrast between the NE-striking relief of the central segment, and the ENE- and ~N-S-striking relief in the two plunging zones. We combine our results with published low-temperature thermochronology and growth strata results to propose a three-stage evolution for the Xiongpo fault-fold belt that closely relates with regional stress field changes, including a NE-striking fault under the NW–SE compression between 40–25 Ma and 15–10 Ma, lateral propagation of the NE-striking fault and initiation of ENE-striking fault by WNW–ESE compression from ~5–2 Ma, ~N–S fault under ~E–W compression until the present. This work enhances our understanding of the stress field changes of eastern Tibet since the Late Eocene. It also can serve as a typical case study deciphering 3D fault-fold growth using seismic and geological imaging, which is helpful to understand 3D structural and landscape evolutions of other complex fault-fold belts worldwide.
Oxidative Dissolution of Low-Grade Ni-Cu Ore and Impact on Flotation of Pentlandite
Authors: Musuku Benjamin , Saari Eija , Dahl Olli
Published: November 2022

This paper investigated the effect of mineral surface oxidation on the floatability of Kevitsa low-grade Ni-Cu ore. Physicochemical measurements, ethylene diamine tetra acetic acid (EDTA) extraction, and oxygen uptake experiments were carried out with slurry and recycled process water samples obtained from the Kevitsa Cu-Ni sequential concentrator plant. The pH of recycled process water, copper flotation feed, and nickel flotation feed dropped by 0.7, 0.4, and 0.7 points, respectively, from May to July. The oxygen demand increased from recycled process water to the copper flotation feed, then dropped for the nickel flotation feed. The nickel flotation feed Redox potential (ORP) was lowest for July, while EDTA extractable metals increased from May to July. There was a 20% drop in nickel recoveries from May to July. Based on ORP measurements of the nickel flotation feed, good nickel flotation takes place in a moderately oxidizing (75–170 mV) and alkaline (9.2–9.7 pH) environment. Therefore, the ORP/pH of the nickel flotation feed is important to the nickel flotation. The results showed that at the Kevitsa plant, the grinding process is an electrochemically active environment, which, together with the incoming recycled process water quality, defines the degree of mineral surface oxidation for flotation. The increasing corrosiveness of the recycled process water increased mineral surface oxidation and depressed pentlandite flotation. Laboratory flotation experiments confirmed the observed poor plant flotation response when the corrosiveness of recycled process water increased. Total dissolved solids (TDS) was proven to be a reliable online parameter for the corrosiveness of the recycled process water and was inversely proportional to the pentlandite recovery. The findings of this study may help the plant develop ways to enable a timely response to changes in recycled process water quality to prevent harmful impacts on pentlandite flotation.
In Situ Nitrogen Functionalization of 2D-Ti3C2Tx-MXenes for High-Performance Zn-Ion Supercapacitor
Authors: Mateen Abdul , Ansari Mohd Zahid , Abbas Qasim , Muneeb Ahmed , Hussain Ahmad , Eldin Elsayed tag , Alzahrani Fatimah Mohammed , Alsaiari Norah Salem , Ali Shafaqat , Javed Muhammad Sufyan
Published: November 2022

Zinc (Zn) ion supercapacitors (ZISCs) have attracted considerable attention as a viable energy storage technology because they are cost-effective, safe, and environmentally friendly. However, cathode materials with suitable properties are rare and need to be explored. In this regard, metal carbides (MXenes) are a good choice for capacitive energy storage, but they exhibit low capacitance. The energy storage performance of MXenes can be bossed using functionalization with heteroatom doping, e.g., nitrogen (N), to simultaneously modify ZISCs’ fundamental characteristics and electrochemical properties. Herein, we present an in-situ N-functionalization of Ti3C2Tx-MXene via a hydrothermal reaction with urea (denoted as N-Ti3C2Tx-MXene). N-functionalization into Ti3C2Tx-MXene raised Ti3C2Tx-MXene’s interlayer spacing and boosted the Zn-ion storage in 1 M ZnSO4 electrolyte. The N-Ti3C2Tx-MXene electrode delivered an excellent specific capacitance of 582.96 F/g at 1 A/g and retained an outstanding cycle stability of 94.62% after 5000 cycles at 10 A/g, which is 1.8 times higher than pristine Ti3C2Tx-MXene at identical conditions. Moreover, the N-Ti3C2Tx-MXene//Zn device demonstrated a maximum capacitance of 153.55 F/g at 1 A/g, retained 92% of its initial value after 5000 cycles, and its Coulombic efficiency was ~100%. This strategy considerably reduced Ti3C2Tx-MXene nanosheet restacking and aggregation and enhanced electrochemical performance. Further, this research elucidated N-Ti3C2Tx-MXene’s charge–storage process and offered a fresh approach to the rational design of novel electrode materials for ZISCs.
Substitutional Diversity-Oriented Synthesis and In Vitro Anticancer Activity of Framework-Integrated Estradiol-Benzisoxazole Chimeras
Authors: Kovács Ferenc , Adamecz Dóra Izabella , Nagy Ferenc István , Papp Benedek , Kiricsi Mónika , Frank Éva
Published: November 2022

Hybridization of steroids and other pharmacophores often modifies the bioactivity of the parent compounds, improving selectivity and side effect profile. In this study, estradiol and 3′-(un)substituted benzisoxazole moieties were combined into novel molecules by structural integration of their aromatic rings. Simple estrogen starting materials, such as estrone, estradiol and estradiol-3-methylether were used for the multistep transformations. Some of the heterocyclic derivatives were prepared from the estrane precursor by a formylation or Friedel–Crafts acylation—oximation—cyclization sequence, whereas others were obtained by a functional group interconversion strategy. The antiproliferative activities of the synthesized compounds were assessed on various human cervical, breast and prostate cancer cell lines (HeLa, MCF-7, PC3, DU-145) and non-cancerous MRC-5 fibroblast cells. Based on the primary cytotoxicity screens, the most effective cancer-selective compounds were selected, their IC50 values were determined and their apoptosis-inducing potential was evaluated by quantitative real-time PCR. Pharmacological studies revealed a strong structure–function relationship, where derivatives with a hydroxyl group on C-17 exhibited stronger anticancer activity compared to the 17-acetylated counterparts. The present study concludes that novel estradiol-benzisoxazole hybrids exert remarkable cancer cell-specific antiproliferative activity and trigger apoptosis in cancer cells.
Lysinated Multiwalled Carbon Nanotubes with Carbohydrate Ligands as an Effective Nanocarrier for Targeted Doxorubicin Delivery to Breast Cancer Cells
Authors: Thakur Chanchal Kiran , Neupane Rabin , Karthikeyan Chandrabose , Ashby Charles R. , Babu R. Jayachandra , Boddu Sai H. S. , Tiwari Amit K. , Moorthy Narayana Subbiah Hari Narayana
Published: November 2022

Multiwalled carbon nanotubes (MWCNTs) are elongated, hollow cylindrical nanotubes made of sp2 carbon. MWCNTs have attracted significant attention in the area of drug delivery due to their high drug-loading capacity and large surface area. Furthermore, they can be linked to bioactive ligands molecules via covalent and noncovalent bonds that allow for the targeted delivery of anticancer drugs such as doxorubicin. The majority of methodologies reported for the functionalization of MWCNTs for drug delivery are quite complex and use expensive linkers and ligands. In the present study, we report a simple, cost-effective approach for functionalizing MWCNTs with the carbohydrate ligands, galactose (GA), mannose (MA) and lactose (LA), using lysine as a linker. The doxorubicin (Dox)-loaded functionalized MWCNTs were characterized using FT-IR, NMR, Raman, XRD and FE-SEM. The drug–loaded MWCNTs were evaluated for drug loading, drug release and cell toxicity in vitro, in breast cancer cells. The results indicated that the carbohydrate-modified lysinated MWCNTs had greater Dox loading capacity, compared to carboxylated MWCNTs (COOHMWCNTs) and lysinated MWCNTs (LyMWCNTs). In vitro drug release experiments indicated that the carbohydrate functionalized LyMWCNTs had higher Dox release at pH 5.0, compared to the physiological pH of 7.4, over 120 h, indicating that they are suitable candidates for targeting the tumor microenvironment as a result of their sustained release profile of Dox. Doxorubicin-loaded galactosylated MWCNTs (Dox-GAMWCNTs) and doxorubicin loaded mannosylated MWCNTs (Dox-MAMWCNTs) had greater anticancer efficacy and cellular uptake, compared to doxorubicin–loaded lactosylated MWCNTs (Dox-LAMWCNTs) and pure Dox, in MDA-MB231 and MCF7 breast cancer cells. However, neither the ligand conjugated multiwall blank carbon nanotubes (GAMWCNTs, MAMWCNTs and LAMWCNTs) nor the lysinated multiwalled blank carbon nanotubes produced significant toxicity in the normal cells. Our results suggest that sugar-tethered multiwalled carbon nanotubes, especially the galactosylated (Dox-GAMWCNTs) and mannosylated (Dox-MAMWCNTs) formulations, may be used to improve the targeted delivery of anticancer drugs to breast cancer cells.
Synthesis of New 2,3-Dihydroindole Derivatives and Evaluation of Their Melatonin Receptor Binding Affinity
Authors: Volkova Maria S. , Efremov Alexander M. , Bezsonova Elena N. , Tsymliakov Michael D. , Maksutova Anita I. , Salykina Maria A. , Sosonyuk Sergey E. , Shevtsova Elena F. , Lozinskaya Natalia A.
Published: November 2022

2,3-Dihydroindoles are promising agents for the synthesis of new compounds with neuroprotective and antioxidant properties. Usually, these compounds are obtained by direct reduction of the corresponding indoles containing acceptor groups in the indole ring for its activation. In this work, we propose a synthetic strategy to obtain new 2,3-dihydroindole derivatives from the corresponding polyfunctional 2-oxindoles. Three methods were proposed for reduction of functional groups in the 2-oxindole and 2-chloroindole molecules using various boron hydrides. The possibility of chemoselective reduction of the nitrile group in the presence of an amide was shown. The proposed synthetic strategy can be used, for example, for the synthesis of new analogs of the endogenous hormone melatonin and other compounds with neuroprotective properties.
Ginger Constituent 6-Shogaol Attenuates Vincristine-Induced Activation of Mouse Gastroesophageal Vagal Afferent C-Fibers
Authors: Patil Mayur J. , Huang Yongming , Yu Mingwei , Dong Xinzhong , Undem Bradley J. , Yu Shaoyong
Published: November 2022

Chemotherapeutic agent-induced nausea and vomiting are the severe adverse effects that are induced by their stimulations on the peripheral and/or central emetic nerve pathways. Even though ginger has been widely used as an herbal medicine to treat emesis, mechanisms underlying its neuronal actions are still less clear. The present study aimed to determine the chemotherapeutic agent vincristine-induced effect on gastroesophageal vagal afferent nerve endings and the potential inhibitory role of ginger constituent 6-shogaol on such response. Two-photon neuron imaging studies were performed in ex vivo gastroesophageal-vagal preparations from Pirt-GCaMP6 transgenic mice. Vincristine was applied to the gastroesophageal vagal afferent nerve endings, and the evoked calcium influxes in their intact nodose ganglion neuron somas were recorded. The responsive nodose neuron population was first characterized, and the inhibitory effects of 5-HT3 antagonist palonosetron, TRPA1 antagonist HC-030031, and ginger constituent 6-shogaol were then determined. Vincristine application at gastroesophageal vagal afferent nerve endings elicited intensive calcium influxes in a sub-population of vagal ganglion neurons. These neurons were characterized by their positive responses to P2X2/3 receptor agonist α,β-methylene ATP and TRPA1 agonist cinnamaldehyde, suggesting their nociceptive placodal nodose C-fiber neuron lineages. Pretreatment with TRPA1 selective blocker HC-030031 inhibited vincristine-induced calcium influxes in gastroesophageal nodose C-fiber neurons, indicating that TRPA1 played a functional role in mediating vincristine-induced activation response. Such inhibitory effect was comparable to that from 5-HT3 receptor antagonist palonosetron. Alternatively, pretreatment with ginger constituent 6-shogaol significantly attenuated vincristine-induced activation response. The present study provides new evidence that chemotherapeutic agent vincristine directly activates vagal nodose nociceptive C-fiber neurons at their peripheral nerve endings in the upper gastrointestinal tract. This activation response requires both TRPA1 and 5-HT3 receptors and can be attenuated by ginger constituent 6-shogaol.
Evidence of the Beneficial Effects of Ursolic Acid against Lung Cancer
Authors: Kornel Amanda , Nadile Matteo , Tsiani Evangelia
Published: November 2022

Lung cancer is the leading cause of cancer-related deaths globally. Despite current treatment approaches that include surgery, chemotherapy, radiation and immunotherapies, lung cancer accounted for 1.79 million deaths worldwide in 2020, emphasizing the urgent need to find novel agents and approaches for more effective treatment. Traditionally, chemicals derived from plants, such as paclitaxel and docetaxel, have been used in cancer treatment, and in recent years, research has focused on finding other plant-derived chemicals that can be used in the fight against lung cancer. Ursolic acid is a polyphenol found in high concentrations in cranberries and other fruits and has been demonstrated to have anti-inflammatory, antioxidant and anticancer properties. In this review, we summarize recent research examining the effects of ursolic acid and its derivatives on lung cancer. Data from in vitro cell culture and in vivo animal studies show potent anticancer effects of ursolic acid and indicate the need for clinical studies.
Carbon Dioxide Adsorption over Activated Carbons Produced from Molasses Using H2SO4, H3PO4, HCl, NaOH, and KOH as Activating Agents
Authors: Kiełbasa Karolina , Bayar Şahin , Varol Esin Apaydin , Sreńscek-Nazzal Joanna , Bosacka Monika , Miądlicki Piotr , Serafin Jarosław , Wróbel Rafał J. , Michalkiewicz Beata
Published: November 2022

Cost-effective activated carbons for CO2 adsorption were developed from molasses using H2SO4, H3PO4, HCl, NaOH, and KOH as activating agents. At the temperature of 0 °C and a pressure of 1 bar, CO2 adsorption equal to 5.18 mmol/g was achieved over activated carbon obtained by KOH activation. The excellent CO2 adsorption of M-KOH can be attributed to its high microporosity. However, activated carbon prepared using HCl showed quite high CO2 adsorption while having very low microporosity. The absence of acid species on the surface promotes CO2 adsorption over M-HCl. The pore size ranges that are important for CO2 adsorption at different temperatures were estimated. The higher the adsorption temperature, the more crucial smaller pores were. For 1 bar pressure and temperatures of 0, 10, 20, and 30 °C, the most important were pores equal and below: 0.733, 0.733, 0.679, and 0.536 nm, respectively.
Advances in Asymmetric Wettable Janus Materials for Oil–Water Separation
Authors: Zhang Jingjing , Wang Congcong , Xing Huwei , Fu Qian , Niu Chenxi , Lu Lingbin
Published: November 2022

The frequent occurrence of crude oil spills and the indiscriminate discharge of oily wastewater have caused serious environmental pollution. The existing separation methods have some defects and are not suitable for complex oil–water emulsions. Therefore, the efficient separation of complex oil–water emulsions has been of great interest to researchers. Asymmetric wettable Janus materials, which can efficiently separate complex oil–water emulsions, have attracted widespread attention. This comprehensive review systematically summarizes the research progress of asymmetric wettable Janus materials for oil–water separation in the last decade, and introduces, in detail, the preparation methods of them. Specifically, the latest research results of two-dimensional Janus materials, three-dimensional Janus materials, smart responsive Janus materials, and environmentally friendly Janus materials for oil–water separation are elaborated. Finally, ongoing challenges and outlook for the future research of asymmetric wettable Janus materials are presented.
First Evidence of a Combination of Terpinen-4-ol and α-Terpineol as a Promising Tool against ESKAPE Pathogens
Authors: Johansen Bianca , Duval Raphaël , Sergere Jean-Christophe
Published: November 2022

Antimicrobial resistance is a major public health issue raising growing concern in the face of dwindling response options. It is therefore urgent to find new anti-infective molecules enabling us to fight effectively against ever more numerous bacterial infections caused by ever more antibiotic-resistant bacteria. In this quest for new antibacterials, essential oils (or compounds extracted from essential oils) appear to be a promising therapeutic option. In the present work, we investigate the potential antibacterial synergy between a combination of terpinen-4-ol and α-terpineol (10:1) compared to standard tea tree oil. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined. Then, time kill assays, in vitro cytotoxicity and bactericidal activity on latent bacteria (persisters) were investigated. Finally, an in silico study of the pharmacokinetic parameters of α-terpineol was also performed. Altogether, our data demonstrate that the combination of terpinen-4-ol and α-terpineol might be a precious weapon to address ESKAPE pathogens.
Interactive Effect of Biological Agents Chitosan, Lentinan and Ningnanmycin on Papaya Ringspot Virus Resistance in Papaya (Carica papaya L.)
Authors: Fan Heling , Yan Xingxiang , Fu Mingqing , Liu Difa , Awan Abdul Waheed , Chen Ping , Rasheed Syed Majid , Gao Ling , Zhang Rongping
Published: November 2022

The papaya industry is mainly impacted by viral diseases, especially papaya ringspot disease (PRSD) caused by papaya ringspot virus (PRSV). So far, research on the interaction between Chitosan, Lentinan and Ningnanmycin on PRSD has not been reported. This research studied the controlled and interactive effect of three biological agents, namely, Chitosan (C), Lentinan (L) and Ningnanmycin (N), on PRSV in papaya, individually and collectively. The changes in disease index, controlled effect, Peroxidase (POD), Polyphenol oxidase (PPO), Superoxide dismutase (SOD), growth and development of plants were observed at the seedling stage, in pots, and at the fruiting stage, in the field. The appearance and nutrient contents of fruits were measured during the fruit stage. The disease index of PRSV, at seedling and fruiting stages, was significantly lower for chitosan, lentinan and ningnanmycin and their interactive effect, compared to a control check treatment. The activity of the defense enzymes could be improved by the three kinds of biological agents and their interactive effect, especially lentinan and ningnanmycin. The chlorophyll content, plant height, stem diameter and fruit quality rose significantly under chitosan, lentinan and ningnanmycin treatments. The interaction of LN could inhibit PRSV disease at the seedling and fruiting stages of papaya, and promote the growth of plants and the quality of fruit at the fruit stage. Hence, this study provides the theoretical foundation for the biological control of papaya ringspot disease.
Unveiling a New Selenocyanate as a Multitarget Candidate with Anticancer, Antileishmanial and Antibacterial Potential
Authors: Ramos-Inza Sandra , Henriquez-Figuereo Andreina , Moreno Esther , Berzosa Melibea , Encío Ignacio , Plano Daniel , Sanmartín Carmen
Published: November 2022

Currently, cancer, leishmaniasis and bacterial infections represent a serious public health burden worldwide. Six cinnamyl and benzodioxyl derivatives incorporating selenium (Se) as selenocyanate, diselenide, or selenide were designed and synthesized through a nucleophilic substitution and/or a reduction using hydrides. Ferrocene was also incorporated by a Friedel–Crafts acylation. All the compounds were screened in vitro for their antiproliferative, antileishmanial, and antibacterial properties. Their capacity to scavenge free radicals was also assessed as a first approach to test their antioxidant activity. Benzodioxyl derivatives 2a–b showed cytotoxicity against colon (HT-29) and lung (H1299) cancer cell lines, with IC50 values below 12 µM, and were also fairly selective when tested in nonmalignant cells. Selenocyanate compounds 1–2a displayed potent antileishmanial activity in L. major and L. infantum, with IC50 values below 5 µM. They also exhibited antibacterial activity in six bacterial strains, notably in S. epidermidis with MIC and MBC values of 12.5 µg/mL. Ferrocene-containing selenide 2c was also identified as a potent antileishmanial agent with radical scavenging activity. Remarkably, derivative 2a with a selenocyanate moiety was found to act as a multitarget compound with antiproliferative, leishmanicidal, and antibacterial activities. Thus, the current work showed that 2a could be an appealing scaffold to design potential therapeutic drugs for multiple pathologies.
Mixed Cultures of Saccharomyces kudravzevii and S. cerevisiae Modify the Fermentation Process and Improve the Aroma Profile of Semi-Sweet White Wines
Authors: Błaszczyk Urszula , Satora Paweł , Noga Łukasz
Published: November 2022

The purpose of the study was to evaluate the impact of the Saccharomyces cerevisiae and S. kudriavzevii mixed culture on the fermentation, chemical and aromatic composition of semi-sweet white wines. The variables tested in the experiment were the initial ratio of yeast in mixed cultures and the time of inoculation of the S. kudriavzevii co-culture. The addition of S. kudriavzevii to the inoculum did not significantly change the chemical composition of the wines obtained. No reduction in ethanol yield was found in mixed culture fermented wines; however, in some variants of the experiment, the ethanol content was higher. The mixed cultures of S. cerevisiae and S. kudriavzevii increased the level of volatile compounds in white grape wines. Wines fermented with the co-culture of S. kudriavzevii were characterized by a more diversified ester profile. The mixed cultures of S. cerevisiae and S. kudriavzevii raised the levels of terpenes in white wines. The most promising results were obtained for mixed culture variants, in which S. kudriavzevii was sequentially inoculated on the sixth day of fermentation.
Recent Achievements in Electrochemical and Optical Nucleic Acids Based Detection of Metal Ions
Authors: Jarczewska Marta , Szymczyk Anna , Zajda Joanna , Olszewski Marcin , Ziółkowski Robert , Malinowska Elżbieta
Published: November 2022

Recently nucleic acids gained considerable attention as selective receptors of metal ions. This is because of the possibility of adjusting their sequences in new aptamers selection, as well as the convenience of elaborating new detection mechanisms. Such a flexibility allows for easy utilization of newly emerging nanomaterials for the development of detection devices. This, in turn, can significantly increase, e.g., analytical signal intensity, both optical and electrochemical, and the same can allow for obtaining exceptionally low detection limits and fast biosensor responses. All these properties, together with low power consumption, make nucleic acids biosensors perfect candidates as detection elements of fully automatic portable microfluidic devices. This review provides current progress in nucleic acids application in monitoring environmentally and clinically important metal ions in the electrochemical or optical manner. In addition, several examples of such biosensor applications in portable microfluidic devices are shown.
Design, Synthesis, and Bioassay of 2′-Modified Kanamycin A
Authors: Yan Ribai , Li Xiaonan , Liu Yuheng , Ye Xinshan
Published: November 2022

Chemical modification of old drugs is an important way to obtain new ones, and it has been widely used in developing new aminoglycoside antibiotics. However, many of the previous modifying strategies seem arbitrary for their lack of support from structural biological detail. In this paper, based on the structural information of aminoglycoside and its drug target, we firstly analyzed the reason that some 2′-N-acetylated products of aminoglycosides caused by aminoglycoside-modifying enzyme AAC(2′) can partially retain activity, and then we designed, synthesized, and evaluated a series of 2′-modified kanamycin A derivatives. Bioassay results showed our modifying strategy was feasible. Our study provided valuable structure–activity relationship information, which would help researchers to develop new aminoglycoside antibiotics more effectively.
Applications of Nanomaterials in Microbial Fuel Cells: A Review
Authors: Abd-Elrahman Nabil. K. , Al-Harbi Nuha , Basfer Noor M. , Al-Hadeethi Yas , Umar Ahmad , Akbar Sheikh
Published: November 2022

Microbial fuel cells (MFCs) are an environmentally friendly technology and a source of renewable energy. It is used to generate electrical energy from organic waste using bacteria, which is an effective technology in wastewater treatment. The anode and the cathode electrodes and proton exchange membranes (PEM) are important components affecting the performance and operation of MFC. Conventional materials used in the manufacture of electrodes and membranes are insufficient to improve the efficiency of MFC. The use of nanomaterials in the manufacture of the anode had a prominent effect in improving the performance in terms of increasing the surface area, increasing the transfer of electrons from the anode to the cathode, biocompatibility, and biofilm formation and improving the oxidation reactions of organic waste using bacteria. The use of nanomaterials in the manufacture of the cathode also showed the improvement of cathode reactions or oxygen reduction reactions (ORR). The PEM has a prominent role in separating the anode and the cathode in the MFC, transferring protons from the anode chamber to the cathode chamber while preventing the transfer of oxygen. Nanomaterials have been used in the manufacture of membrane components, which led to improving the chemical and physical properties of the membranes and increasing the transfer rates of protons, thus improving the performance and efficiency of MFC in generating electrical energy and improving wastewater treatment.
Synthesis, Characterization and DFT Study of a New Family of High-Energy Compounds Based on s-Triazine, Carborane and Tetrazoles
Authors: Makarenkov Anton V. , Kiselev Sergey S. , Kononova Elena G. , Dolgushin Fedor M. , Peregudov Alexander S. , Borisov Yurii A. , Ol’shevskaya Valentina A.
Published: November 2022

An efficient one-pot synthesis of carborane-containing high-energy compounds was developed via the exploration of carbon–halogen bond functionalization strategies in commercially available 2,4,6-trichloro-1,3,5-triazine. The synthetic pathway first included the substitution of two chlorine atoms in s-triazine with 5-R-tetrazoles (R = H, Me, Et) units to form disubstituted tetrazolyl 1,3,5-triazines followed by the sequential substitution of the remaining chlorine atom in 1,3,5-triazine with carborane N- or S-nucleophiles. All new compounds were characterized by IR- and NMR spectroscopy. The structure of four new compounds was confirmed by single crystal X-ray diffraction analysis. The density functional theory method (DFT B3LYP/6-311 + G*) was used to study the geometrical structures, enthalpies of formation (EOFs), energetic properties and highest occupied and lowest unoccupied molecular orbital (HOMO and LUMO) energies and the detonation properties of synthesized compounds. The DFT calculation revealed compounds processing the maximum value of the detonation velocity or the maximum value of the detonation pressure. Theoretical terahertz frequencies for potential high-energy density materials (HEDMs) were computed, which allow the opportunity for the remote detection of these compounds.
New Pyrimidine-5-Carbonitriles as COX-2 Inhibitors: Design, Synthesis, Anticancer Screening, Molecular Docking, and In Silico ADME Profile Studies
Authors: AL-Ghulikah Hanan A. , El-Sebaey Samiha A. , Bass Amr K. A. , El-Zoghbi Mona S.
Published: November 2022

Two series of cyanopyrimidine hybrids were synthesized bearing either benzo[d]imidazole, benzo[d]oxazole, benzo[d]thiazole, and benzo[b]thiophene derivatives via methylene amino linker 3a–3d (Formula A) or various sulphonamide phenyl moieties 5a–5d (Formula B) at the C-2 position. All compounds’ cyclooxygenase COX-2 inhibitory activities were evaluated, and all synthesized compounds demonstrated potent activity at minimal concentrations, with IC50 values in the submicromolar range. Compounds 3b, 5b, and 5d were discovered to be the most active pyrimidine derivatives, with the highest COX-2 percent inhibition and IC50 values being nearly equal to Celecoxib and approximately 4.7-, 9.3-, and 10.5-fold higher than Nimesulide. Furthermore, the pyrimidine derivatives 3b, 5b, and 5d demonstrated anticancer activity comparable to or better than doxorubicin against four cell lines, i.e., MCF-7, A549, A498, and HepG2, with IC50 values in nanomolar in addition to low cytotoxicity on the normal W38-I cell line. The effect of compound 5d on cell cycle progression and apoptosis induction was investigated, and it was found that compound 5d could seize cell growth at the sub-G1 and G2/M phases, as well as increase the proportion of early and late apoptotic rates in MCF-7 cells by nearly 13- and 60-fold, respectively. Moreover, in silico studies for compounds 3b, 5b, and 5d revealed promising findings, such as strong GIT absorption, absence of BBB permeability, nil-to-low drug–drug interactions, good oral bioavailability, and optimal physicochemical properties, indicating their potential as promising therapeutic candidates.
High-Temperature Magnesiothermic Reduction Enables HF-Free Synthesis of Porous Silicon with Enhanced Performance as Lithium-Ion Battery Anode
Authors: Zuo Xiuxia , Yang Qinghua , He Yaolong , Cheng Ya-Jun , Yin Shanshan , Zhu Jin , Müller-Buschbaum Peter , Xia Yonggao
Published: November 2022

Porous silicon-based anode materials have gained much interest because the porous structure can effectively accommodate volume changes and release mechanical stress, leading to improved cycling performance. Magnesiothermic reduction has emerged as an effective way to convert silica into porous silicon with a good electrochemical performance. However, corrosive HF etching is normally a mandatory step to improve the electrochemical performance of the as-synthesized silicon, which significantly increases the safety risk. This has become one of the major issues that impedes practical application of the magnesiothermic reduction synthesis of the porous silicon anode. Here, a facile HF-free method is reported to synthesize macro-/mesoporous silicon with good cyclic and rate performance by simply increasing the reduction temperature from 700 °C to 800 °C and 900 °C. The mechanism for the structure change resulting from the increased temperature is elaborated. A finite element simulation indicated that the 3D continuous structure formed by the magnesiothermic reduction at 800 °C and 900 °C could undertake the mechanical stress effectively and was responsible for an improved cyclic stability compared to the silicon synthesized at 700 °C.
Antihypothyroid Effect of Salidroside
Authors: Korbozova Nazym K. , Kudrina Nataliya O. , Zhukova Nataliya A. , Grazhdannikov Alexander E. , Blavachinskaya Irina V. , Seitimova Gulnaz A. , Kulmanov Timur E. , Tolstikova Tatyana G. , Terletskaya Nina V.
Published: November 2022

In terms of prevalence, thyroid pathology, associated both with a violation of the gland function and changes in its structure, occupies one of the main places in clinical endocrinology. The problem of developing low-toxic and highly effective herbal preparations for the correction of thyroid hypofunction and its complications is urgent. Salidroside is a glucoside of tyrosol, found mostly in the roots of Rhodiola spp., and has various positive biological activities. The purpose of this study was to study the antihypothyroid potential of salidrosid-containing extract from R. semenovii roots, which was evaluated on a mercazolyl hypothyroidism model. We showed that extract containing salidroside is a safe and effective means of hypothyroidism correction, significantly reducing (p ≤ 0.001) the level of thyroid-stimulating hormone and increasing the level of thyroid hormones. The combined use of R. semenovii extract with potassium iodide enhances the therapeutic effect of the extract by 1.3-times.
Novel In Situ Growth of ZIF-8 in Porous Epoxy Matrix for Mechanically Robust Composite Electrolyte of High-Performance, Long-Life Lithium Metal Batteries
Authors: Zhang Wenjie , Long Jianlin , Wang Haijun , Lan Jinle , Yu Yunhua , Yang Xiaoping
Published: November 2022

Polymer electrolytes (PEs) with high flexibility, low cost, and excellent interface compatibility have been considered as an ideal substitute for traditional liquid electrolytes for high safety lithium metal batteries (LMBs). Nevertheless, the mechanical strength of PEs is generally poor to prevent the growth of lithium dendrites during the charge/discharge process, which seriously restricts their wide practical applications. Herein, a mechanical robust ZIF-8/epoxy composite electrolyte with unique pore structure was prepared, which effectively inhibited the growth of lithium dendrites. Meanwhile, the in situ growth of ZIF-8 in porous epoxy matrix can promote the uniform flux and fast transport of lithium ions. Ultimately, the optimal electrolyte shows high ionic conductivity (2.2 × 10−3 S cm−1), wide electrochemical window (5 V), and a large Li+ transference number (0.70) at room temperature. The Li||NCM811 cell using the optimal electrolyte exhibits high capacity and excellent cycling performance (83.2% capacity retention with 172.1 mA h g−1 capacity retained after 200 cycles at 0.2 C). These results indicate that the ZIF-8/epoxy composite electrolyte is of great promise for the application in LMBs.
Modification of Polymeric Carbon Nitride with Au–CeO2 Hybrids to Improve Photocatalytic Activity for Hydrogen Evolution
Authors: Zhang Linzhu , Chen Lu , Xia Yuzhou , Liang Zhiyu , Huang Renkun , Liang Ruowen , Yan Guiyang
Published: November 2022

The construction of a multi-component heterostructure for promoting the exciton splitting and charge separation of conjugated polymer semiconductors has attracted increasing attention in view of improving their photocatalytic activity. Here, we integrated Au nanoparticles (NPs) decorated CeO2 (Au–CeO2) with polymeric carbon nitride (PCN) via a modified thermal polymerization method. The combination of the interfacial interaction between PCN and CeO2 via N-O or C-O bonds, with the interior electronic transmission channel built by the decoration of Au NPs at the interface between CeO2 and PCN, endows CeAu–CN with excellent efficiency in the transfer and separation of photo-induced carriers, leading to the enhancement of photochemical activity. The amount-optimized CeAu–CN nanocomposites are capable of producing ca. 80 μmol· H2 per hour under visible light irradiation, which is higher than that of pristine CN, Ce–CN and physical mixed CeAu and PCN systems. In addition, the photocatalytic activity of CeAu–CN remains unchanged for four runs in 4 h. The present work not only provides a sample and feasible strategy to synthesize highly efficient organic polymer composites containing metal-assisted heterojunction photocatalysts, but also opens up a new avenue for the rational design and synthesis of potentially efficient PCN-based materials for efficient hydrogen evolution.
Potential Anti-Tumor Activity of Nardoguaianone L Isolated from Nardostachys jatamansi DC. in SW1990 Cells
Authors: Sang Chun-Yan , Zheng Yi-Dan , Ma Li-Mei , Wang Kai , Wang Cheng-Bo , Chai Tian , Eshbakova Komila A. , Yang Jun-Li
Published: November 2022

Natural products (NPs) were a rich source of diverse bioactive molecules. Most anti-tumor agents were built on natural scaffolds. Nardostachys jatamansi DC. was an important plant used to process the traditional Chinese herbal medicines “gansong”. Pancreatic cancer was the fourth most common cause of cancer-related death in the world. Hence, there was an urgent need to develop novel agents for the treatment of pancreatic cancer. In this paper, nardoguaianone L (G-6) is isolated from N. jatamansi, which inhibited SW1990 cells colony formation and cell migration, and induced cell apoptosis. Furthermore, we analyzed the differential expression proteins after treatment with G-6 in SW1990 cells by using iTRAQ/TMT-based quantitative proteomics technology, and the results showed that G-6 regulated 143 proteins’ differential expression by GO annotation, including biological process, cellular component, and molecular function. Meanwhile, KEGG enrichment found that with Human T-cell leukemia virus, one infection was the most highly enhanced pathway. Furthermore, the MET/PTEN/TGF-β pathway was identified as a significant pathway that had important biological functions, including cell migration and motility by PPI network analysis in SW1990 cells. Taken together, our study found that G-6 is a potential anti-pancreatic cancer agent with regulation of MET/PTEN/TGF-β pathway.
Phenolic Profile, EPR Determination, and Antiproliferative Activity against Human Cancer Cell Lines of Anthyllis vulneraria Extracts
Authors: Ouerfelli Manel , Metón Isidoro , Codina-Torrella Idoia , Almajano María Pilar
Published: November 2022

In the current work, the leaf and flower extracts of Anthyllis vulneraria were evaluated for their chemical characterization using HPLC-MS and for their radical scavenging capacity toward methoxy radicals produced by a Fenton-type reaction using an electron paramagnetic resonance (EPR) spectroscopy assay. The in vitro antiproliferative activity of these extracts against several human-derived cancer cells (breast: MCF-7; cervical: HeLa; hepatocellular: HepG2) was also evaluated. The results showed that the Anthyllis vulneraria leaf extract was characterized by 17 different phenolic compounds, among which phenolic acids were the most abundant, while its flower extract exhibited higher contents of flavonoids. Furthermore, Anthyllis vulneraria extracts demonstrated a potent radical scavenging activity against methoxy radicals. Both extracts also significantly reduced the viability of the different cancer cell lines. The results of the current study suggested that Anthyllis vulneraria extracts are a promising source of antioxidant compounds with health benefits and pointed to their potential use for treating cancer and developing novel therapeutic agents.
Authenticity Assessment from Sesame Seeds to Oil and Sesame Products of Various Origin by Differential Scanning Calorimetry
Authors: Rajagukguk Yolanda Victoria , Utcu Mert Atakan , Islam Mahbuba , Muzolf-Panek Małgorzata , Tomaszewska-Gras Jolanta
Published: November 2022

The aim of this study was to conduct thermal characterization of sesame seeds and oils from various geographical origins (Ethiopia, India, Nigeria, Sudan, Turkey), different method of extraction (hexane and cold-pressing), and different types of derived products (halva and tahini). Thermal characterization was investigated using differential scanning calorimetry (DSC), which showed that origin of the seeds has no influence on the melting profile of sesame oil (peak temperature and enthalpy). Method of extraction (hexane and cold-pressing) influenced the peak temperatures of the resulting oils (p ≤ 0.05). The addition of 20% of palm olein to pure sesame oil influenced the significant changes in thermodynamic parameters such as peak temperature (Tm2), which was lowered from −5.89 °C to −4.99 °C, peak half width (T1/2), elevated from 3.01 °C to 4.52 °C, and the percentage of first peak area (% peak 1) lowered from 87.9 to 73.2% (p ≤ 0.05). The PCA method enabled to distinguish authentic and adulterated sesame oils of various origins. There were no significant differences in thermal properties among the products (halva, tahini) and the authentic sesame oil (p > 0.05). The obtained results showed DSC feasibility to characterize sesame oil and sesame products in terms of authenticity.
A Two-Stage Culture Strategy for Scenedesmus sp. FSP3 for CO2 Fixation and the Simultaneous Production of Lutein under Light and Salt Stress
Authors: Li Jiawei , Zhao Xinqing , Chang Jo-Shu , Miao Xiaoling
Published: November 2022

In this study, Scenedesmus sp. FSP3 was cultured using a two-stage culture strategy for CO2 fixation and lutein production. During the first stage, propylene carbonate was added to the medium, with 5% CO2 introduced to promote the rapid growth and CO2 fixation of the microalgae. During the second stage of cultivation, a NaCl concentration of 156 mmol L−1 and a light intensity of 160 μmol m−2 s−1 were used to stimulate the accumulation of lutein in the microalgal cells. By using this culture method, high lutein production and CO2 fixation were simultaneously achieved. The biomass productivity and carbon fixation rate of Scenedesmus sp. FSP3 reached 0.58 g L−1 d−1 and 1.09 g L−1 d−1, with a lutein content and yield as high as 6.45 mg g−1 and 2.30 mg L−1 d−1, respectively. The results reveal a commercially feasible way to integrate microalgal lutein production with CO2 fixation processes.