bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytosociological Analysis of the Boreal Oligotrophic Pine Forests in the Southern Ural Region (Russia)
Authors: Martynenko Vasiliy , Shirokikh Pavel , Baisheva Elvira , Muldashev Albert , Fedorov Nikolay , Zhigunova Svetlana , Naumova Leniza
Published: August 2024

Floristic composition and syntaxonomy of the boreal oligotrophic pine forests covering vast areas in the boreal, hemiboreal, and temperate zones of continental Eurasia still remain poorly studied in the Southern Ural region. Using the Braun–Blanquet approach and TURBOVEG and JUICE software, the phytocoenotic diversity of boreal oligotrophic pine forests of the Southern Ural region was studied, and their position in the system of ecological and floristic classification of Eurasian vegetation was determined. Geobotanical data on boreal oligotrophic pine forests of Europe, including the European part of Russia; the Southern Urals; and Siberia were compared. A new alliance of oligotrophic boreal pine forests Brachypodio pinnati-Pinion sylvestris all. nov. hoc loco is described. The communities of this new alliance (i.e., five associations from the Southern Ural region) are characterized by a special floristic composition, occupying an intermediate position between the typical European oligotrophic pine forests of the alliance Dicrano-Pinion (Libbert 1933) Matuszkiewicz 1962 and oligotrophic (mainly psammophilous) South Siberian pine forests of the alliance Hieracio-Pinion Anenkhonov et Chytrý 1998. The communities of the alliance Brachypodio pinnati-Pinion sylvestris prefer to grow on poor soils with different moisture conditions. Due to intensive forestry activities, the distribution area of these forests has decreased, and these communities have been replaced by secondary birch forests. We have proposed a set of conservation measures to preserve these communities. A new association of oligotrophic pine forests Psephello sumensis-Pinetum sylvestris ass. nov. hoc loco is also described. These communities from the Kurgan region of Western Siberia were ordered into the alliance Dicrano-Pinion. It confirms the idea that the distribution area of this alliance may reach Siberia. Unlike the Southern Ural pine forests of the alliance Brachypodio pinnati-Pinion sylvestris, the recovery of these West Siberian pine forests after felling is quite high, and these communities do not require special measures for their protection.
Determinants of Deadwood Biomass under the Background of Nitrogen and Water Addition in Warm Temperate Forests
Authors: Hong Liang , Tang Shouzheng , Li Tao , Fu Liyong , Song Xinyu , Duan Guangshuang , Fu Jueming , Ma Lei
Published: August 2024

Climate change is exacerbating the vulnerability of temperate forests to severe disturbances, potentially increasing tree mortality rates. Despite the significance of this issue, there has been a lack of comprehensive research on tree survival across extensive forest areas under the background of global climate change. To fill this gap, we conducted a detailed analysis of tree survival within a canopy nitrogen and water addition experimental platform in central China, utilizing data from two censuses and evaluating contributing factors. Our findings revealed 283 dead trees within the plots, predominantly of very small diameters (1–10 cm). The distribution of these dead trees varied among subplots, influenced by both biotic and abiotic factors. Notably, three dominant tree species were responsible for 64.8% of the deadwood biomass. The study determined that both the breast diameter and the quantity of dead trees, affected by surrounding trees and environmental conditions, played a critical role in deadwood biomass accumulation. This research offers an in-depth examination of deadwood biomass patterns in a temperate forest, highlighting the need to consider both experiment treatments and abiotic elements like topography in studies of forest ecosystem carbon. The insights gained from this study enhance our understanding of warm temperate forests’ role in the global carbon cycle and offer valuable guidance for forest conservation and management strategies.
A Survey on the Use of Large Language Models (LLMs) in Fake News
Authors: Papageorgiou Eleftheria , Chronis Christos , Varlamis Iraklis , Himeur Yassine
Published: August 2024

The proliferation of fake news and fake profiles on social media platforms poses significant threats to information integrity and societal trust. Traditional detection methods, including rule-based approaches, metadata analysis, and human fact-checking, have been employed to combat disinformation, but these methods often fall short in the face of increasingly sophisticated fake content. This review article explores the emerging role of Large Language Models (LLMs) in enhancing the detection of fake news and fake profiles. We provide a comprehensive overview of the nature and spread of disinformation, followed by an examination of existing detection methodologies. The article delves into the capabilities of LLMs in generating both fake news and fake profiles, highlighting their dual role as both a tool for disinformation and a powerful means of detection. We discuss the various applications of LLMs in text classification, fact-checking, verification, and contextual analysis, demonstrating how these models surpass traditional methods in accuracy and efficiency. Additionally, the article covers LLM-based detection of fake profiles through profile attribute analysis, network analysis, and behavior pattern recognition. Through comparative analysis, we showcase the advantages of LLMs over conventional techniques and present case studies that illustrate practical applications. Despite their potential, LLMs face challenges such as computational demands and ethical concerns, which we discuss in more detail. The review concludes with future directions for research and development in LLM-based fake news and fake profile detection, underscoring the importance of continued innovation to safeguard the authenticity of online information.
Challenges in Assessing Repellency via the Behavioral Response by the Global Pest Tribolium castaneum to Protect Stored Grains
Authors: Rault Leslie C. , Morrison William R. , Gerken Alison R. , Bingham Georgina V.
Published: August 2024

Background: Food security is an increasingly pressing global issue, and by 2050, food production will not be sufficient to feed the growing population. Part of global food insecurity can be attributed to post-harvest losses, including quantity and quality losses caused by stored-product pests like insects. It is thus timely to find management strategies to mitigate these losses and counteract food insecurity. The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), a global stored-product pest with a wide range of food sources, was used in this study to assess repellency to a selection of essential oils. Methods: Multiple behaviorally relevant methods were used to determine the efficacy of the essential oils in assays to pinpoint the most promising repellents. Experiments were used to assess individual and group behaviors with or without airflow and examined the behavioral variation in distance moved and the time spent away from the oil. Results: It was found that exposure to essential oils and conditions of experimentation considerably influenced T. castaneum’s behavioral response, but a clear candidate for repellency could not be chosen based on the collected data. Conclusions: Follow-up research is needed to pinpoint repellents for integrated pest management practices to protect grains from stored-product pests and to justify their use in and around commodities.
Use of Wing Geometric Morphometric Analysis and mtDNA to Identify Africanization of Apis mellifera in the Central Highlands of Ecuador
Authors: Masaquiza Diego , Rodríguez Lino Curbelo , Zapata José , Monar Joffre , Vaca Maritza , Porrini Leonardo , Eguaras Martin , Daniele Martin , Romero Dora , Arenal Amilcar
Published: August 2024

Seventy-five samples were collected from 15 beehives in the central highlands of Ecuador (Tungurahua–Chimborazo) to assess Africanization in managed bee populations using wing geometric morphometric and mitochondrial DNA analyses. The results indicated that when grouping the apiaries based on altitudinal floors into 2600–2800, 2801–3000, and 3001–3274 m above sea level, differences (p < 0.001) were observed. The morphotypes were similar in the first two floors, but the third indicated that altitude plays a crucial role in the differentiation of populations. When comparing with the pure subspecies, we found differences (p < 0.001); the nearest Mahalanobis distance was for Apis mellifera scutellata (D2 = 3.51), with 95.8% Africanization via father in the area. The maternal origin of all patterns belonged to lineage A (A. m. scutellata), with seven haplotypes. The most frequent haplotypes were A26 and A1; however, the A1q haplotype was not detected at the national level or in nearby countries. The identified haplotypes do not coincide with A4, which is predominant in South Africa and Brazil. The results indicate a double origin due to their presence in North Africa and the Iberian Peninsula. The formation of specific morphological groups within ecoregions is suggested.
Surgery Combined with Local Implantation of Doxorubicin-Functionalized Hydroxyapatite Halts Tumor Growth and Prevents Bone Destruction in an Aggressive Osteosarcoma
Authors: Liu Yang , Corbascio Tova , Huang Jintian , Engellau Jacob , Lidgren Lars , Tägil Magnus , Raina Deepak Bushan
Published: August 2024

Osteosarcoma treatment comprises pre-surgical chemotherapy followed by radical surgery and further chemotherapy cycles, but the prognosis has been far from satisfactory. No new drugs or treatment modalities have been developed for clinical use in the last four decades. We describe a nano-hydroxyapatite (HA)-based local drug delivery platform for the delivery of doxorubicin (DOX), a cornerstone drug in osteosarcoma treatment. The efficacy of the developed drug delivery system was evaluated in an orthotopic human osteosarcoma xenograft in the proximal tibia of mice. After tumor development, the tumor was surgically resected and the void filled with the following: (1) No treatment (G1); (2) nHA only (G2); (3) DOX-loaded nHA (G3). In-vivo tumor response was assessed by evaluating the tumor-induced osteolysis at 2 weeks using micro-CT followed by in-vivo PET-CT at 3 weeks and ex-vivo micro-CT and histology. Micro-CT imaging revealed complete destruction of the tibial metaphysis in groups G1 and G2, while the metaphysis was protected from osteolysis in G3. PET-CT imaging using 18F-FDG revealed high metabolic activity in the tumors in G1 and G2, which was significantly reduced in G3. Using histology, we were able to verify that local DOX delivery reduced the bone destruction and the tumor burden compared with G1 and G2. No off-target toxicity in the vital organs could be observed in any of the treatment groups histologically. This study describes a novel local drug adjuvant delivery approach that could potentially improve the prognosis for patients responding poorly to the current osteosarcoma treatment.
Towards the Wall or the Bridge? A Case Study of Host–Guest Symbiosis in a Chinese Heritage Tourism Site
Authors: Tao Hui , Chen Xiaoying , Sun Yehong , Wang Zhe
Published: August 2024

The close connection between community residents and tourists in heritage tourism sites strengthens the relationship between people and places. To explore the mechanisms of host–guest interaction and the driving factors of residents’ willingness to participate in tourism in heritage tourism destinations, this study adopts a mixed-method approach combining qualitative research and quantitative analysis. The results of the study show that host–guest symbiosis in heritage tourism destinations goes through four stages: identity qualification, bodily co-presence, common focus, and emotional solidarity. Quantitative research (SEM) reveals the impact of residents’ welcoming nature, emotional closeness, and sympathetic understanding towards tourists on the sense of community belonging, as well as the subsequent response of willingness to participate in tourism. Through the analysis of the host–guest interaction mechanisms and symbiotic relationships in heritage tourism sites, the key emotional factors influencing residents’ participation in tourism are revealed.
In Situ Synthesis of (Mo,Cr)Si2 Composites by Spark Plasma Sintering
Authors: Wang Yue-Yao , Zhang Guo-Hua
Published: August 2024

This research investigated the impact of Cr content on the properties of (Mo,Cr)Si2 composites. Composites with CrSi2 molar fractions ranging from 0% to 10% were fabricated using spark plasma sintering (SPS). The study undertook a systematic analysis of the surface morphology, phase composition, mechanical properties, and high-temperature oxidation resistance of the sintered samples across different compositions. Notably, the (Mo95,Cr5)Si2 composite sintered at 1400 °C exhibited enhanced properties, including a Vickers hardness of 11.6 GPa, a fracture toughness of 4.6 MPa·m1/2, and a flexural strength of 397 MPa. Upon oxidation at 1500 °C, the (Mo,Cr)Si2 composites formed a protective oxide layer comprised of SiO2 and Cr2O3. It was found that the generation and thickening of the protective oxide layer was promoted by the addition of moderate amounts of Cr to MoSi2.
Sliding Shear Failure of Basement-Clamped Reinforced Concrete Shear Walls
Authors: Schuler Harald
Published: August 2024

After an earthquake struck Chile in 1985, sliding shear failure was observed in the clamping zone of stabilising walls. Even houses with only four storeys can fail due to sliding shear below basement ceilings. This type of failure has received little attention in the past; however, it leads to premature failure of the clamping effect in the basement box. Bending deformations cannot fully develop, which significantly reduces the seismic safety; it is thus important to learn more about the sliding shear failure of basement-clamped shear walls. The overall behaviour is analysed based on three large-scale shear wall tests. The deformation states around the sliding shear zone are evaluated and a simple estimate is given of when sliding shear failure can occur.
An Analysis of Changes in the Harmfulness of the Bottle Packaging Process Depending on the Type of Heat-Shrinkable Film
Authors: Walichnowska Patrycja , Kruszelnicka Weronika , Mazurkiewicz Adam , Kłos Zbigniew , Rudawska Anna , Bembenek Michał
Published: August 2024

This article shows an analysis of selected stages of a machine’s life cycle environmental impact in the specific case of machines that package bottles in thermo-shrinkable film. As part of this analysis, laboratory tests were carried out to compare the performance properties of polyethylene films (with and without recycled material). Then, a life cycle assessment (LCA) was carried out within the specified system boundaries using the SimaPro program. Using the ReCiPe 2016 method, differences in the impact of the mass bottle packaging process on the categories human health, ecosystems and resources were determined depending on the shrink film used in the process. These tests showed that the tested batch of film with the addition of recyclates has similar functional properties to traditional ones and can therefore be used in the mass packaging process. The environmental analysis showed that changing the type of film to film with the addition of recyclates results in an almost 70% reduction in the potential negative impact of the process in terms of damage to health and ecosystems, and by 85% in terms of resources.
Effects of Shot Peening Pressure, Time, and Material on the Properties of Carburized Steel Shafts
Authors: Lu Shao-Quan , Chiu Liu-Ho , Chang Pei-Jung , Lin Chung-Kwei
Published: August 2024

Carburized steel shafts are commonly used in industry due to their good wear resistance and fatigue life. If the surface of carburized shafts exhibits an undesired tensile stress, shot peening treatment may be required to alter the stress condition on the surface. In the present study, the effects of shot peening pressure (3–5 kg/cm2), time (32–64 s), and material (stainless steel, carbon steel, and glass) on the residual stress, retained austenite, microhardness, and surface roughness of the carburized shafts were investigated. The experimental results showed that the surface residual tensile stress was changed into compressive stress after the shot peening treatment. The shot peening effects increased with the increasing peening pressure and time. In addition, a significant decrease in the amount of retained austenite in the subsurface region was observed. Peening with different materials can affect the peening effect. Using glass pellets exhibited the best shot peening effect but suffered massive pellet fracture during processing. In overall consideration, the optimal peening parameters for carburized steel shafts for practical industrial applications involved using the stainless-steel pellets with a peening pressure of 5 kg/cm2 and a peening time of 64 s. The maximum residual stress was −779 MPa at a depth of 0.02 mm, while the highest surface microhardness was 827 HV0.1.
Antioxidant and Skin-Whitening Efficacy of a Novel Decapeptide (DP, KGYSSYICDK) Derived from Fish By-Products
Authors: Lee Sung-Gyu , Hwang Jin-Woo , Kang Hyun
Published: August 2024

The skin is vulnerable to damage from ultraviolet rays and oxidative stress, which can lead to aging and pigmentation issues. This study investigates the antioxidant and whitening efficacy of a decapeptide (DP, KGYSSYICDK) derived from marine fish by-products and evaluates its potential as a new skin-whitening agent. DP demonstrated high antioxidant activity, showing comparable or superior performance to Vitamin C (Vit. C) in ferric reducing antioxidant power (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. In hydrogen peroxide (H2O2)-treated HaCaT cells, DP increased cell viability and reduced reactive oxygen species (ROS) generation. Furthermore, DP inhibited tyrosinase activity and decreased melanin production in α-melanocyte stimulating hormone (α-MSH)-induced B16F10 melanoma cells in a dose-dependent manner. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that DP reduces the mRNA expression of MITF, tyrosinase, and MC1R, thus suppressing melanin production. DP exhibits strong binding interactions with multiple amino acid residues of tyrosinase, indicating potent inhibitory effects on the enzyme. These results suggest that DP possesses significant antioxidant and whitening properties, highlighting its potential as a skin-whitening agent. Future research should focus on optimizing DP’s structure and exploring structure–activity relationships.
Metabolite Predictors of Breast and Colorectal Cancer Risk in the Women’s Health Initiative
Authors: Navarro Sandi L. , Williamson Brian D. , Huang Ying , Nagana Gowda G. A. , Raftery Daniel , Tinker Lesley F. , Zheng Cheng , Beresford Shirley A. A. , Purcell Hayley , Djukovic Danijel , Gu Haiwei , Strickler Howard D. , Tabung Fred K. , Prentice Ross L. , Neuhouser Marian L. , Lampe Johanna W.
Published: August 2024

Metabolomics has been used extensively to capture the exposome. We investigated whether prospectively measured metabolites provided predictive power beyond well-established risk factors among 758 women with adjudicated cancers [n = 577 breast (BC) and n = 181 colorectal (CRC)] and n = 758 controls with available specimens (collected mean 7.2 years prior to diagnosis) in the Women’s Health Initiative Bone Mineral Density subcohort. Fasting samples were analyzed by LC-MS/MS and lipidomics in serum, plus GC-MS and NMR in 24 h urine. For feature selection, we applied LASSO regression and Super Learner algorithms. Prediction models were subsequently derived using logistic regression and Super Learner procedures, with performance assessed using cross-validation (CV). For BC, metabolites did not increase predictive performance over established risk factors (CV-AUCs~0.57). For CRC, prediction increased with the addition of metabolites (median CV-AUC across platforms increased from ~0.54 to ~0.60). Metabolites related to energy metabolism: adenosine, 2-hydroxyglutarate, N-acetyl-glycine, taurine, threonine, LPC (FA20:3), acetate, and glycerate; protein metabolism: histidine, leucic acid, isoleucine, N-acetyl-glutamate, allantoin, N-acetyl-neuraminate, hydroxyproline, and uracil; and dietary/microbial metabolites: myo-inositol, trimethylamine-N-oxide, and 7-methylguanine, consistently contributed to CRC prediction. Energy metabolism may play a key role in the development of CRC and may be evident prior to disease development.
Mantle Volatiles and Heat Contributions to the Cu-Pb-Zn Mineralization in the Baoshan Deposit, South China: Constraints from He and Ar Isotopes
Authors: Huang Jinchuan , Peng Jiantang , Xie Tengxiang
Published: August 2024

The Baoshan deposit is one of the important Cu-Pb-Zn deposits associated with granitic rocks in the Nanling Range, South China. Here, we present He and Ar isotope data for the Baoshan deposit to decipher the contributions of mantle-derived volatiles and heat to its Cu-Pb-Zn mineralization. The ore-forming fluids in sphalerite and pyrite exhibited 3He/4He ratios up to 1.51 Ra. A linear correlation between He and Ar isotopes suggests that the ore-forming fluids were a mixture of a predominantly mantle-derived fluid with a high 3He/4He ratio and a shallow crustal fluid, characterized by a low 3He/4He ratio. The δ34S values of sulfides in the Baoshan deposit ranged from +2.30 to +5.21‰, consistent with the magma-derived sulfur. The calculated 3He/Q ratios for the ore-forming fluid exceeded those of mid-oceanic ridge hydrothermal solutions by 10 to 50 times, indicating that the ore-forming fluids acquired both heat and volatiles in a convective hydrothermal regime rather than a conductive one. Therefore, there is a significant contribution of mantle-derived volatiles, heat, and possibly metals, to the Cu-Pb-Zn mineralization in the Baoshan deposit, and the continuous influx of mantle-derived fluids/melts probably plays a crucial role during the Cu-Pb-Zn mineralization related to granitic rocks.
Egg Consumption and 4-Year Change in Cognitive Function in Older Men and Women: The Rancho Bernardo Study
Authors: Kritz-Silverstein Donna , Bettencourt Ricki
Published: August 2024

The effect of dietary cholesterol on cognitive function is debatable. While eggs contain high levels of dietary cholesterol, they provide nutrients beneficial for cognitive function. This study examined the effects of egg consumption on change in cognitive function among 890 ambulatory adults (N = 357 men; N = 533 women) aged ≥55 years from the Rancho Bernardo Study who attended clinic visits in 1988–1991 and 1992–1996. Egg intake was obtained in 1988–1991 with a food frequency questionnaire. The Mini-Mental Status Exam (MMSE), Trails B, and category fluency were administered at both visits to assess cognitive performance. Sex-specific multiple regression analyses tested associations of egg intake with changes in cognitive function after adjustment for confounders. The mean time between visits was 4.1 ± 0.5 years; average ages were 70.1 ± 8.4 in men and 71.5 ± 8.8 in women (p = 0.0163). More men consumed eggs at higher levels than women; while 14% of men and 16.5% of women reported never eating eggs, 7.0% of men and 3.8% of women reported intakes ≥5/week (p = 0.0013). In women, after adjustment for covariates, egg consumption was associated with less decline in category fluency (beta = −0.10, p = 0.0241). Other associations were nonsignificant in women, and no associations were found in men. Results suggest that egg consumption has a small beneficial effect on semantic memory in women. The lack of decline observed in both sexes suggests that egg consumption does not have detrimental effects and may even have a role in the maintenance of cognitive function.
Dietary Isoflavones Intake and Gastric Cancer
Authors: Natale Arianna , Fiori Federica , Parpinel Maria , Pelucchi Claudio , Negri Eva , La Vecchia Carlo , Rossi Marta
Published: August 2024

Dietary isoflavones have been associated with a lower risk of gastric cancer (GC), but the evidence for this association is still limited. We investigated the association between isoflavone intake and GC risk using data from a case–control study including 230 incident, histologically confirmed GC cases and 547 controls with acute, non-neoplastic conditions. Dietary information was collected through a validated food frequency questionnaire (FFQ) and isoflavone intake was estimated using ad hoc databases. We estimated the odds ratios (OR) and the corresponding 95% confidence intervals (CI) of GC using logistic regression models, including terms for total energy intake and other major confounders. The OR for the highest versus the lowest tertile of intake was 0.65 (95%CI = 0.44–0.97, p for trend = 0.04) for daidzein, 0.75 (95%CI = 0.54–1.11, p for trend = 0.15) for genistein, and 0.66 (95%CI = 0.45–0.99, p for trend = 0.05) for total isoflavones. Stratified analyses by sex, age, education, and smoking showed no heterogeneity. These findings indicate a favorable effect of dietary isoflavones on GC.
Dietary Intakes of Copper and Selenium in Association with Bone Mineral Density
Authors: Pasco Julie A. , Anderson Kara B. , Williams Lana J. , Stuart Amanda L. , Hyde Natalia K. , Holloway-Kew Kara L.
Published: August 2024

The important trace elements, copper and selenium, have diverse effects on human health. As well as other important roles in living tissues, these trace elements are toxic at high levels but are key constituents of various enzymes and proteins essential for maintaining physiological health. However, links between dietary intakes of these elements, particularly copper, and bone mineral density (BMD) in humans remain uncertain. This study aimed to investigate whether dietary intakes of copper and selenium are associated with BMD in women. Dietary intakes of copper and selenium were assessed for 575 women in the Geelong Osteoporosis Study, using a detailed semi-quantitative food frequency questionnaire in conjunction with nutrition composition databases. Participants taking oral multivitamin preparations were excluded from analyses; 522 participants (ages 20–88 y) met the eligibility criteria. BMD at multiple skeletal sites was measured by dual energy X-ray absorptiometry (Lunar DPX-L). Separate multivariable regression models were developed to identify associations between copper and selenium intakes and BMD, after adjustments for age, anthropometry, other dietary factors, medication use, and lifestyle factors. Median (interquartile range) daily intake for copper was 1.5 mg (1.2–1.9) and for selenium, 72 μg (57–90). Low intakes (lowest tertile versus pooled upper tertiles) of copper and selenium were consistently associated with lower BMD at multiple skeletal sites. Fully adjusted models identified small but statistically significant differences in BMD, ranging from 1.8% to 4.0% for low copper intakes and 1.4% to 4.0% for low selenium intakes. Low dietary intakes of copper and selenium were both independently associated with lower BMD, at least in this sample of women. The results contribute to the evidence base for informing dietary recommendations for these trace elements with respect to their contributions to optimal bone health.
A Systematic Review of Clinical Practice Guidelines on the Management of Malnutrition in Children with Congenital Heart Disease
Authors: Kołodziej Maciej , Skulimowska Julia
Published: August 2024

Congenital heart disease (CHD) is one of the most common inborn disorders, with a prevalence of 0.8–1.2%. Affected children are often malnourished due to increased dietary requirements. This may lead to severe long-term complications. Several authoritative organizations have published guidelines addressing nutritional intervention in children with CHD. We aimed to systematically assess the consistency of recommendations, the methodological quality of these guidelines, and the quality of evidence supporting each recommendation. PubMed, Embase, the Cochrane Database, World Health Organization Global Index Medicus, and 16 scientific societies’ websites were searched for the period until September 2023. The guideline quality was assessed using the AGREE II tool. After screening 765 records, only 2 guidelines published in 2013 and 2022 met our inclusion criteria. The main reason for exclusion was the absence of any system for rating the evidence. The main issues concerned the lack of implementation advice or tools and the lack of criteria to measure the application of guideline recommendations. The included guidelines were of good quality and within specific recommendations, both publications were largely in agreement, and the score for the overall assessment was high (83%). There is a pressing need for comprehensive, multi-threaded guidelines incorporating implementation strategies and methods for the performance assessment of children with malnutrition and CHD.
Nuclear Receptors and Stress Response Pathways Associated with the Development of Oral Mucositis Induced by Antineoplastic Agents
Authors: Kagaya Moena , Uesawa Yoshihiro
Published: August 2024

Oral mucositis (OM) is one of the common adverse events associated with cancer treatment that decreases the quality of life and affects treatment outcomes. However, the medications used to manage OM are generally only palliative, and our knowledge of the syndrome is limited. The etiology of the syndrome is thought to be complex and multifactorial. We investigated the trends and characteristics of OM and estimated molecular initiating events (MIEs) associated with the development of the syndrome using the FDA Adverse Event Reporting System. The study of trends and characteristics suggested that OM is significantly more likely to occur in females and nonelderly patients and is likely to be induced by protein kinase inhibitors such as afatinib and everolimus. Next, we used Toxicity Predictor, an in-house quantitative structure–activity relationship system, to estimate OM-associated MIEs. The results revealed that the agonist activity of the human pregnane X receptor, thyroid-stimulating hormone-releasing hormone receptor, and androgen receptor may be associated with OM development. Our study findings are expected to help avoid the risk of OM induction during the drug discovery process and clinical use of antineoplastic agents.
A Compound Containing Aldehyde Dehydrogenase Relieves the Effects of Alcohol Consumption and Hangover Symptoms in Healthy Men: An Open-Labeled Comparative Study
Authors: Jeong In-Kyung , Han Anna , Jun Ji Eun , Hwang You-Cheol , Ahn Kyu Jeung , Chung Ho Yeon , Kang Bo Seung , Choung Se-Young
Published: August 2024

This open-labeled and comparative study aimed to test the efficacy and safety of a fermented rice extract-based substance containing yeast-fermented powder having aldehyde dehydrogenase (KisLip®, Pico Entech, Republic of Korea) in healthy male individuals. Healthy male subjects (n = 20) consumed 90 g of alcohol at their first visit. At the second visit, participants consumed 90 g of alcohol or alcohol with a low dose of KISLip® (2000 mg, KL-L) and then 90 g of alcohol or alcohol with a high dose of KISLip® (3000 mg, KL-H) at the third visit. The efficacy of KISLip® depends on the mutational status of important genes related to alcohol metabolism, including alcohol dehydrogenase (ADH1B), cytochrome P4502E1 (CYP2E1 (5B) and CYP2E1 (6)), and aldehyde dehydrogenase (ALDH2). KISLip® significantly reduced the highest level (Cmax) of alcohol and overall levels of acetaldehyde compared to the alcohol-only group in a dose-dependent manner. These significant effects of KISLip® on alcohol metabolism were observed independent of mutations in the four genes. In addition, hangover symptoms were significantly decreased in the KISLip® treated groups. During the study, the participants did not show any adverse events after KISLip® intake. This clinical study suggested that supplementation of KISLip® had beneficial effects on alcohol metabolism and might ameliorate the severity of hangovers without any adverse events.
Antitumor Effects and the Potential Mechanism of 10-HDA against SU-DHL-2 Cells
Authors: Tian Yuanyuan , Liu Xiaoqing , Wang Jie , Zhang Chuang , Yang Wenchao
Published: August 2024

10-hydroxy-2-decenoic acid (10-HDA), which is a unique bioactive fatty acid of royal jelly synthesized by nurse bees for larvae and adult queen bees, is recognized for its dual utility in medicinal and nutritional applications. Previous research has indicated that 10-HDA exerts antitumor effects on numerous tumor cell lines, including colon cancer cells, A549 human lung cancer cells, and human hepatoma cells. The present study extends this inquiry to lymphoma, specifically evaluating the impact of 10-HDA on the SU-DHL-2 cell line. Our findings revealed dose-dependent suppression of SU-DHL-2 cell survival, with an IC50 of 496.8 μg/mL at a density of 3 × 106 cells/well after 24 h. For normal liver LO2 cells and human fibroblasts (HSFs), the IC50 values were approximately 1000 μg/mL and over 1000 μg/mL, respectively. The results of label-free proteomics revealed 147 upregulated and 347 downregulated differentially expressed proteins that were significantly enriched in the complement and coagulation cascades pathway (adjusted p-value = 0.012), including the differentially expressed proteins prothrombin, plasminogen, plasminogen, carboxypeptidase B2, fibrinogen beta chain, fibrinogen gamma chain, and coagulation factor V. The top three hub proteins, ribosomal protein L5, tumor protein p53, and ribosomal protein L24, were identified via protein–protein interaction (PPI) analysis. This result showed that the complement and coagulation cascade pathways might play a key role in the antitumor process of 10-HDA, suggesting a potential therapeutic avenue for lymphoma treatment. However, the specificity of the effect of 10-HDA on SU-DHL-2 cells warrants further investigation.
A Comparative Study of the Rapid (IKr) and Slow (IKs) Delayed Rectifier Potassium Currents in Undiseased Human, Dog, Rabbit, and Guinea Pig Cardiac Ventricular Preparations
Authors: Ágoston Márta , Kohajda Zsófia , Virág László , Baláti Beáta , Nagy Norbert , Lengyel Csaba , Bitay Miklós , Bogáts Gábor , Vereckei András , Papp Julius Gy. , Varró András , Jost Norbert
Published: August 2024

To understand the large inter-species variations in drug effects on repolarization, the properties of the rapid (IKr) and the slow (IKs) components of the delayed rectifier potassium currents were compared in myocytes isolated from undiseased human donor (HM), dog (DM), rabbit (RM) and guinea pig (GM) ventricles by applying the patch clamp and conventional microelectrode techniques at 37 °C. The amplitude of the E-4031-sensitive IKr tail current measured at −40 mV after a 1 s long test pulse of 20 mV, which was very similar in HM and DM but significant larger in RM and GM. The L-735,821-sensitive IKs tail current was considerably larger in GM than in RM. In HM, the IKs tail was even smaller than in DM. At 30 mV, the IKr component was activated extremely rapidly and monoexponentially in each studied species. The deactivation of the IKr component in HM, DM, and RM measured at −40 mV. After a 30 mV pulse, it was slow and biexponential, while in GM, the IKr tail current was best fitted triexponentially. At 30 mV, the IKs component activated slowly and had an apparent monoxponential time course in HM, DM, and RM. In contrast, in GM, the activation was clearly biexponential. In HM, DM, and RM, IKs component deactivation measured at −40 mV was fast and monoexponential, while in GM, in addition to the fast component, another slower component was also revealed. These results suggest that the IK in HM resembles that measured in DM and RM and considerably differs from that observed in GM. These findings suggest that the dog and rabbit are more appropriate species than the guinea pig for preclinical evaluation of new potential drugs expected to affect cardiac repolarization.
The Association between Vitamin D Status and the Impact of Metformin on Hypothalamic–Pituitary–Thyroid Axis Activity in Women with Subclinical Hypothyroidism
Authors: Krysiak Robert , Kowalcze Karolina , Szkróbka Witold , Okopień Bogusław
Published: August 2024

Metformin inhibits enhanced secretion of anterior pituitary hormones. Its impact on prolactin and gonadotropin concentrations is absent in individuals with hypovitaminosis D. The aim of this prospective cohort study was to investigate whether vitamin D status determines the effect of metformin on hypothalamic–pituitary–thyroid axis activity in levothyroxine-naïve women. The study included three groups of women of reproductive age with subclinical non-autoimmune hypothyroidism, which were matched for age, thyroid-stimulating hormone (TSH) concentration, and insulin sensitivity: untreated women with vitamin D deficiency/insufficiency (group A), women effectively supplemented with exogenous calciferol (group B), and untreated women with normal 25-hydroxyvitamin D concentrations (25OHD) (group C). Owing to concomitant type 2 diabetes or prediabetes, all subjects were treated with metformin. Concentrations of 25OHD, TSH, total and free thyroid hormones, glucose, insulin, glycated hemoglobin (HbA1c), prolactin, and peripheral markers of thyroid hormone action were assayed before metformin treatment and six months later. Based on hormone concentration, structure parameters of thyroid homeostasis were calculated. Except for 25OHD concentrations, there were no between-group differences in baseline values of the measured variables. Metformin reduced glucose, the homeostatic model assessment 1 of insulin resistance ratio (HOMA1-IR), and HbA1c in all study group, but these effects were less pronounced in group A than in the remaining groups. The reduction in TSH and Jostel’s index was observed only in groups B and C, and its degree correlated with baseline TSH concentrations, baseline 25OHD concentrations, and the degree of improvement in HOMA1-IR. The drug did not affect circulating levels of 25OHD, free and total thyroid hormones, prolactin, other structure parameters of thyroid homeostasis, and markers of thyroid hormone action. The obtained results allow us to conclude that low vitamin D status in young women mitigates the impact of metformin on thyrotroph secretory function.
Anti-Inflammatory, Antibacterial, Anti-Biofilm, and Anti-Quorum Sensing Activities of the Diterpenes Isolated from Clinopodium bolivianum
Authors: Apaza Ticona Luis , Martínez Noguerón Ana , Sánchez Sánchez-Corral Javier , Montoto Lozano Natalia , Ortega Domenech Monserrat
Published: August 2024

This study reports for the first time the isolation of four diterpenoid compounds: 15-Hydroxy-12-oxo-abietic acid (1), 12α-hydroxyabietic acid (2), (−)-Jolkinolide E (3), and 15-Hydroxydehydroabietic acid (4) from Clinopodium bolivianum (C. bolivianum). The findings demonstrate that both the dichloromethane/methanol (DCMECB) extract of C. bolivianum and the isolated compounds exhibit significant anti-inflammatory (inhibition of NF-κB activation), antibacterial (primarily against Gram-positive bacteria), and anti-biofilm (primarily against Gram-negative bacteria) activities. Among the isolated diterpenes, compounds 3 and 4 showed notable anti-inflammatory effects, with IC50 values of 17.98 μM and 23.96 μM for compound 3, and 10.79 μM and 17.37 μM for compound 4, in the HBEC3-KT and MRC-5 cell lines. Regarding their antibacterial activity, compounds 3 and 4 were particularly effective, with MIC values of 0.53–1.09 μM and 2.06–4.06 μM, respectively, against the S. pneumoniae and S. aureus Gram-positive bacteria. Additionally, these compounds demonstrated significant anti-biofilm and anti-quorum sensing activities, especially against Gram-negative bacteria (H. influenzae and L. pneumophila). We also explain how compound 3 (BIC = 1.50–2.07 μM, Anti-QS = 0.31–0.64 μM) interferes with quorum sensing due to its structural homology with AHLs, while compound 4 (BIC = 4.65–7.15 μM, Anti-QS = 1.21–2.39 μM) destabilises bacterial membranes due to the presence and position of its hydroxyl groups. These results support the traditional use of C. bolivianum against respiratory infections caused by both Gram-positive and Gram-negative bacteria. Furthermore, given the increasing antibiotic resistance and biofilm formation by these bacteria, there is a pressing need for the development of new, more active compounds. In this context, compounds 3 and 4 isolated from C. bolivianum offer promising potential for the development of a library of new, more potent, and selective drugs.
Mechanical, Biological and In Vitro Degradation Investigation of Braided Scaffolds for Tendon and Ligament Tissue Engineering Based on Different Polycaprolactone Materials with Chitosan-Graft-PCL Surface Modification
Authors: Emonts Caroline , Bauer Benedict , Pitts Johannes , Roger Yvonne , Hoffmann Andrea , Menzel Henning , Gries Thomas
Published: August 2024

Injuries to tendons and ligaments are highly prevalent in the musculoskeletal system. Current treatments involve autologous transplants with limited availability and donor site morbidity. Tissue engineering offers a new approach through temporary load-bearing scaffolds. These scaffolds have to fulfill numerous requirements, the majority of which can be met using braiding combined with high-strength polycaprolactone (PCL) fibers. Considering regulatory requirements, several medical-grade PCL materials were assessed regarding their mechanical, degradational and cell biological properties. In the course of the investigation, an excellent fiber tensile strength of up to 850 MPa was achieved. The fibers were braided into multilayer scaffolds and scaled to match the human ACL. These were characterized regarding their morphology and their mechanical and degradational properties. Two strategies were followed to provide biological cues: (a) applying a chitosan-graft-PCL surface modification and (b) using non-circular fiber morphologies as topographical stimuli. Cell vitality assays showed generally positive cytocompatibility and no impairments due to the surface modification or material grade. The best cell vitality was achieved with a scaffold consisting of snowflake-shaped monofilaments combined with a 25° braiding angle. The surface modification equips the scaffold with a release platform for function molecules (as recently demonstrated) so that a holistic approach to addressing the numerous requirements is provided.
Rewilding as Re-Enchantment
Authors: De Vroey Linde
Published: August 2024

Rewilding is regularly connected to re-enchantment. In some rewilding projects, ecological restoration merges with narratives of wonder, enchantment and spirituality. While rewilding’s association with re-enchantment appears as part of its appeal, it is also criticised as anthropocentric, escapist or naive. This article aims to formulate a thorough conceptual understanding of rewilding as re-enchantment by situating it within the critical framework of (dis)enchantment developed in the early 20th century by Weber and Horkheimer and Adorno. Connecting this framework to contemporary, phenomenologically inspired accounts of enchantment and critiques on the mechanisation of nature, this article aims towards a new perspective on rewilding as a critical discursive practice of re-enchantment. Rewilding, like re-enchantment, can be seen as a valuable attempt to formulate alternatives to the modern paradigm and programme of disenchantment. Situating rewilding within a broader cultural context and historical perspective, this approach allows for assessing rewilding as part of modern (counter-)culture at large. Moreover, through a detailed account of (re-)enchantment in rewilding on a phenomenological, theoretical and relational level, this article gradually develops a conceptual understanding of re-enchantment as a valuable concept for ecological restoration and cultural transformation.
Study on the Estimation of Leaf Area Index in Rice Based on UAV RGB and Multispectral Data
Authors: Zhang Yuan , Jiang Youyi , Xu Bo , Yang Guijun , Feng Haikuan , Yang Xiaodong , Yang Hao , Liu Changbin , Cheng Zhida , Feng Ziheng
Published: August 2024

Leaf area index (LAI) is a key variable for monitoring crop growth. Compared with traditional measurement methods, unmanned aerial vehicle (UAV) remote sensing offers a cost-effective and efficient approach for rapidly obtaining crop LAI. Although there is extensive research on rice LAI estimation, many studies suffer from the limitations of models that are only applicable to specific scenarios with unclear applicability conditions. In this study, we selected commonly used RGB and multispectral (Ms) data sources, which contain three channels of color information and five multi-band information, respectively, combined with five different spatial resolutions of data at intervals of 20–100 m. We evaluated the effectiveness of models using single- and multi-feature variables for LAI estimation in rice. In addition, texture and coverage features other than spectra were introduced to further analyze their effects on the inversion accuracy of the LAI. The results show that the accuracy of the model established with multi-variables under single features is significantly higher than that of the model established with single variables under single features. The best results were obtained using the RFR (random forest regression) model, in which the model’s R2 is 0.675 and RMSE is 0.886 for multi-feature VIs at 40 m. Compared with the analysis results of Ms and RGB data at different heights, the accuracy of Ms data estimation results fluctuates slightly and is less sensitive to spatial resolution, while the accuracy of the results based on RGB data gradually decreases with the increase in height. The estimation accuracies of both Ms and RGB data were improved by adding texture features and coverage features, and their R2 improved by 9.1% and 7.3% on average. The best estimation heights (spatial resolution) of the two data sources were 40 m (2.2 cm) and 20 m (0.4 cm), with R2 of 0.724 and 0.673, and RMSE of 0.810 and 0.881. This study provides an important reference for the estimation of rice LAI based on RGB and Ms data acquired using the UAV platform.
SOD-YOLO: Small-Object-Detection Algorithm Based on Improved YOLOv8 for UAV Images
Authors: Li Yangang , Li Qi , Pan Jie , Zhou Ying , Zhu Hongliang , Wei Hongwei , Liu Chong
Published: August 2024

The rapid development of unmanned aerial vehicle (UAV) technology has contributed to the increasing sophistication of UAV-based object-detection systems, which are now extensively utilized in civilian and military sectors. However, object detection from UAV images has numerous challenges, including significant variations in the object size, changing spatial configurations, and cluttered backgrounds with multiple interfering elements. To address these challenges, we propose SOD-YOLO, an innovative model based on the YOLOv8 model, to detect small objects in UAV images. The model integrates the receptive field convolutional block attention module (RFCBAM) in the backbone network to perform downsampling, improving feature extraction efficiency and mitigating the spatial information sparsity caused by downsampling. Additionally, we developed a novel neck architecture called the balanced spatial and semantic information fusion pyramid network (BSSI-FPN) designed for multi-scale feature fusion. The BSSI-FPN effectively balances spatial and semantic information across feature maps using three primary strategies: fully utilizing large-scale features, increasing the frequency of multi-scale feature fusion, and implementing dynamic upsampling. The experimental results on the VisDrone2019 dataset demonstrate that SOD-YOLO-s improves the mAP50 indicator by 3% compared to YOLOv8s while reducing the number of parameters and computational complexity by 84.2% and 30%, respectively. Compared to YOLOv8l, SOD-YOLO-l improves the mAP50 indicator by 7.7% and reduces the number of parameters by 59.6%. Compared to other existing methods, SODA-YOLO-l achieves the highest detection accuracy, demonstrating the superiority of the proposed method.