bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanical assessment of two hybrid plate designs for pancarpal canine arthrodesis under cyclic loading
Authors: Zderic Ivan , Varga Peter , Styger Ursula , Drenchev Ludmil , Gueorguiev Boyko , Asimus Erik , Saunders W. Brian , Kowaleski Michael , Boudrieau Randy J. , Déjardin Loic M.
Published: March 2023

Pancarpal canine arthrodesis (PCA) sets immobilization of all three carpal jointsviadorsal plating to result in bony fusion. Whereas the first version of the plate uses a round hole (RH) for the radiocarpal (RC) screw region, its modification into an oval hole (OH) in a later version improves versatility in surgical application. The aim of this study was to mechanically investigate the fatigue life of the PCA plate types implementing these two features–PCA-RH and PCA-OH. Ten PCA-RH and 20 PCA-OH stainless steel (316LVM) plates were assigned to three study groups (n = 10). All plates were pre-bent at 20° and fixed to a canine forelimb model with simulated radius, RC bone and third metacarpal bone. The OH plates were fixed with an RC screw inserted either most proximal (OH-P) or most distal (OH-D). All specimens were cyclically tested at 8 Hz under 320 N loading until failure. Fatigue life outcome measures were cycles to failure and failure mode. Cycles to failure were higher for RH plate fixation (695,264 ± 344,023)versusboth OH-P (447,900 ± 176,208) and OH-D (391,822 ± 165,116) plate configurations, being significantly different between RH and OH-D,p= 0.03. No significant difference was detected between OH-P and OH-D configurations,p= 0.09. Despite potential surgical advantages, the shorter fatigue life of the PCA-OH plate design may mitigate its benefits compared to the plate design with a round radiocarpal screw hole. Moreover, the failure risk of plates with an oval hole is increased regardless from the screw position in this hole. Based on these findings, the PCA plate with the current oval radiocarpal screw hole configuration cannot be recommended for clinical use.
Fullerenol inhibits tendinopathy by alleviating inflammation
Authors: Jiao Xin , Wang Zengguang , Li Yiming , Wang Tianchang , Xu Chen , Zhou Xianhao , Gan Yaokai
Published: March 2023

Tendinopathy is a common disease in orthopaedics, seriously affecting tendon functions. However, the effects of non-surgical treatment on tendinopathy are not satisfactory and surgical treatments possibly impair the function of tendons. Biomaterial fullerenol has been proved to show good anti-inflammatory effects on various inflammatory diseases. For in vitro experiments, primary rat tendon cells (TCs) were treated by interleukin-1 beta (IL-1β) combined with aqueous fullerenol (5, 1, 0.3 μg/mL). Then inflammatory factors, tendon-related markers, migration and signaling pathways were detected. For in vivo experiments, rat tendinopathy model was constructed by local injection of collagenase into Achilles tendons of rats and fullerenol (0.5, 1 mg/mL) was locally injected 7 days after collagenase injection. Inflammatory factors and tendon-related markers were also investigated. Fullerenol with good water-solubility showed excellent biocompatibility with TCs. Fullerenol could increase expression of tendon-related factors (Collagen I and tenascin C) and decrease expression of inflammatory factors (matrix metalloproteinases-3, MMP-3, and MMP-13) and reactive oxygen species (ROS) level. Simultaneously, fullerenol slowed the migration of TCs and inhibited activation of Mitogen-activated protein kinase (MAPK) signaling pathway. Fullerenol also attenuated tendinopathy in vivo, including reduction of fiber disorders, decrease of inflammatory factors and increase of tendon markers. In summary, fullerenol is a promising biomaterial that can be used to treat tendinopathy.
Maternal vaginal microbiome composition does not affect development of the infant gut microbiome in early life
Authors: Dos Santos Scott J. , Pakzad Zahra , Albert Arianne Y. K. , Elwood Chelsea N. , Grabowska Kirsten , Links Matthew G. , Hutcheon Jennifer A. , Maan Evelyn J. , Manges Amee R. , Dumonceaux Tim J. , Hodgson Zoë G. , Lyons Janet , Mitchell-Foster Sheona M. , Gantt Soren , Joseph K.S. , Van Schalkwyk Julie E. , Hill Janet E. , Money Deborah M.
Published: March 2023

Birth mode has been implicated as a major factor influencing neonatal gut microbiome development, and it has been assumed that lack of exposure to the maternal vaginal microbiome is responsible for gut dysbiosis among caesarean-delivered infants. Consequently, practices to correct dysbiotic gut microbiomes, such as vaginal seeding, have arisen while the effect of the maternal vaginal microbiome on that of the infant gut remains unknown. We conducted a longitudinal, prospective cohort study of 621 Canadian pregnant women and their newborn infants and collected pre-delivery maternal vaginal swabs and infant stool samples at 10-days and 3-months of life. Using cpn60-based amplicon sequencing, we defined vaginal and stool microbiome profiles and evaluated the effect of maternal vaginal microbiome composition and various clinical variables on the development of the infant stool microbiome. Infant stool microbiomes showed significant differences in composition by delivery mode at 10-days postpartum; however, this effect could not be explained by maternal vaginal microbiome composition and was vastly reduced by 3 months. Vaginal microbiome clusters were distributed across infant stool clusters in proportion to their frequency in the overall maternal population, indicating independence of the two communities. Intrapartum antibiotic administration was identified as a confounder of infant stool microbiome differences and was associated with lower abundances of Escherichia coli, Bacteroides vulgatus, Bifidobacterium longum and Parabacteroides distasonis. Our findings demonstrate that maternal vaginal microbiome composition at delivery does not affect infant stool microbiome composition and development, suggesting that practices to amend infant stool microbiome composition focus factors other than maternal vaginal microbes.
Evaluation of Mycobacterium avium subsp. paratuberculosis isocitrate lyase (IcL) and ABC transporter (BacA) knockout mutants as vaccine candidates
Authors: Eshraghisamani Razieh , Arrazuria Rakel , Luo Lucy , De Buck Jeroen
Published: March 2023

There has been little success in controlling Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis, due to suboptimal diagnostics and the ineffectiveness of available vaccines. By knocking out BacA and IcL, genes required for MAP survival in dairy calves, two live-attenuated vaccine candidates were created. This study evaluated the host-specific attenuation of MAP IcL and BacA mutants in mouse and calf models, as well as the elicited immune responses. Deletion mutants were generated in MAP strain A1-157 through specialized transduction and found viable in vitro. First, the mutants’ attenuation and elicited cytokine secretion were assessed in a mouse model, 3 weeks after intraperitoneal inoculation with MAP strains. Later, vaccine strains were assessed in a natural host infection model where calves received 109CFU oral dose of MAP wild-type or mutant strains at 2 weeks old. Transcription levels of cytokines in PBMCs were evaluated at 12-, 14-, and 16-weeks post-inoculation (WPI) and MAP colonization in tissue was assessed at 4.5 months after inoculation. Whereas both vaccine candidates colonized mouse tissues similarly to wild-type strain, both failed to persist in calf tissues. In either mouse or calf models, gene deletion did not reduce immunogenicity. Instead, inoculation with ΔBacA induced a greater upregulation of proinflammatory cytokines than ΔIcL and wild-type in both models and a greater expansion of cytotoxic and memory T-cells than uninfected control in calves. ΔBacA and wild-type strains significantly increased secretion of IP-10, MIG, TNFα, and RANTES in mice serum compared to uninfected control. This agreed with upregulation of IL-12, IL-17, and TNFα in calves inoculated with ΔBacA at all time points. The ΔBacA also gave rise to greater populations of CD4+CD45RO+, and CD8+ cells than uninfected control calves at 16 WPI. Low survival rate of MAP in macrophages co-incubated with PBMCs isolated from the ΔBacA group indicated that these cell populations are capable of killing MAP. Overall, the immune response elicited by ΔBacA is stronger compared to ΔIcL and it is maintained over two different models and over time in calves. Further investigation is warranted to evaluate the BacA mutant's protection against MAP infection as a live attenuated vaccine candidate.
A framework for exploring futures of complex urban energy systems
Authors: Basu Sumedha , Bale Catherine S. E.
Published: March 2023

In order to address the climate crisis and provide citizens with clean, secure and affordable energy, urban energy systems need to transition. This is significant as urban energy systems are increasingly seen as complex systems for their close interactions with local urban society, while being interdependent with higher levels of governance. Decisions taken today will continue to influence the inhabitants of our cities for well over 50 years, locking in energy consumption patterns of the future. How, then, do we make decisions on the interventions needed to bring about a desirable future, and prepare for the probable and possible futures? In this paper, we consider the key characteristics of urban energy systems from a complexity science perspective in order to explore what methodologies in futures and foresight scholarship could be beneficial in supporting urban energy decision-making. To do this we have undertaken an integrative review—a method that allows review, synthesis, critique, and analysis of new and emerging topics across multiple disciplines and multiple literature types—and consider the findings in light of their usefulness in understanding complex systems, which are inherently uncertain. We consider how futures and foresight theories and methods can be applied in urban and energy studies, highlighting examples of where around the world these have been applied by organizations seeking to shape transitions. The many methods and approaches that exist under the futures' umbrella have not been applied to anywhere near their full potential in urban energy studies, despite the limitations of many of the planning and modeling exercises currently used. We use key learnings from existing futures and foresight scholarship, along with our understanding of urban energy systems as complex adaptive systems, to propose a theoretical and practical framework for exploring their futures. The framework encompasses concepts of futures, contextualization, mapping uncertainty, participatory processes, and futures governance. Although there is much further research work needed to test and operationalize this framework in an applied way with city stakeholders, we hope this charts a way forward in addressing the critical challenges faced by urban energy planners and their partners.
iNOS expressing macrophages co-localize with nitrotyrosine staining after myocardial infarction in humans
Authors: Wilmes Verena , Kur Ivan M. , Weigert Andreas , Verhoff Marcel A. , Gradhand Elise , Kauferstein Silke
Published: March 2023

Introduction

Inducible nitric oxide synthase (iNOS) produces micromolar amounts of nitric oxide (NO) upon the right stimuli, whose further reactions can lead to oxidative stress. In murine models of myocardial infarction (MI), iNOS is known to be expressed in infiltrating macrophages, which at early onset enter the infarcted zone and are associated with inflammation. In contrast cardiac tissue resident macrophages are thought to enhance regeneration of tissue injury and re-establish homeostasis. Both detrimental and beneficial effects of iNOS have been described, still the role of iNOS in MI is not fully understood. Our aim was to examine cell expression patterns of iNOS and nitrotyrosine (NT) production in human MI.

Material and Methods

We examined in postmortem human MI hearts the iNOS mRNA expression by means of qPCR. Further we performed immunohistochemical stainings for cell type identification. Afterwards a distance analysis between iNOS and NT was carried out to determine causality between iNOS and NT production.

Results

iNOS mRNA expression was significantly increased in infarcted regions of human MI hearts and iNOS protein expression was detected in resident macrophages in infarcted human hearts as well as in controls hearts, being higher in resident macrophages in MI hearts compared to control. Furthermore in MI and in healthy human hearts cells showing signs of NT production peaked within 10–15 µm proximity of iNOS+ cells.

Discussion

These results indicate that, unexpectedly, resident macrophages are the main source of iNOS expression in postmortem human MI hearts. The peak of NT positive cells within 10–15 µm of iNOS+ cells suggest an iNOS dependent level of NT and therefore iNOS dependent oxidative stress. Our results contribute to understanding the role of iNOS in human MI.
Bibliometric and visual analysis of RAN methylation in cardiovascular disease
Authors: Song Boce , Xie Beili , Liu Mingwang , Li Haohao , Shi Dazhuo , Zhao Fuhai
Published: March 2023

Background

RNA methylation is associated with cardiovascular disease (CVD) occurrence and development. The purpose of this study is to visually analyze the results and research trends of global RNA methylation in CVD.

Methods

Articles and reviews on RNA methylation in CVD published before 6 November 2022 were searched in the Web of Science Core Collection. Visual and statistical analysis was performed using CiteSpace 1.6.R4 advanced and VOSviewer 1.6.18.

Results

There were 847 papers from 1,188 institutions and 63 countries/regions. Over approximately 30 years, there was a gradual increase in publications and citations on RNA methylation in CVD. America and China had the highest output (284 and 259 papers, respectively). Nine of the top 20 institutions that published articles were from China, among which Fudan University represented the most. The International Journal of Molecular Sciences was the journal with the most studies. Nature was the most co-cited journal. The most influential writers were Zhang and Wang from China and Mathiyalagan from the United States. After 2015, the primary keywords were cardiac development, heart, promoter methylation, RNA methylation, and N6-methyladenosine. Nuclear RNA, m6A methylation, inhibition, and myocardial infarction were the most common burst keywords from 2020 to the present.

Conclusions

A bibliometric analysis reveals research hotspots and trends of RNA methylation in CVD. The regulatory mechanisms of RNA methylation related to CVD and the clinical application of their results, especially m6A methylation, are likely to be the focus of future research.
A magnetic measurement technique for small rare samples: Pre-study for Chang’e-5 returned and other extraterrestrial samples
Authors: Hou Yifei , Zhang Min , He Kuang , Shen Zhongshan , Dong Liang , Fan Yunchang , Qi Kaixian , Liu Shuangchi , Cai Shuhui , Qin Huafeng
Published: March 2023

Standard-size samples are usually used for traditional paleomagnetic studies. In some cases, small irregular samples are employed considering their amount and magnetic strength as well as experiment efficiency. With the development of deep space exploration, the demand for studying small and magnetic weak samples is increasing. In this study, we established a magnetic measurement technique for small rare samples. We designed low-background, sample-nondestructive and high-flexibility holders with various materials for different measurements of small samples. With the customized holders, we estimated uncertainty and reliability of magnetic measurements of small specimens from a terrestrial basalt. The results indicate that susceptibility and remanence of small specimens (e.g., 2-mm cubes) are more affected by measurement position than larger specimens (≥5 mm), which reminds us to pay special attention to consistency of the small specimen position during series of measurements. Moreover, susceptibility and remanence of small sister specimens (2-mm and 5-mm cubes) are more scattered than those of large ones (cube length ≥1 cm), mainly due to inhomogeneity of magnetic mineral abundance in the sample. Nevertheless, the average magnetic results of different-size specimens are generally consistent within error, indicating the inhomogeneity of small specimens can be averaged out and demonstrating the reliability of small samples during magnetic measurements. Finally, we tested our measurement system with a lunar meteorite, which mimics the Chang’e-5 returned samples in both size and mineralogy. The main minerals of the meteorite are plagioclase, pyroxene, troilite and kamacite. The isothermal remanent magnetization normalized paleointensity method recovered the expected laboratory field while the anhysteretic remanent magnetization corrected paleointensities are about 2-3 times the expected field, where the latter can be explained by inapplicability of the empirical f′ to the meteorite specimens. The anhysteretic remanent magnetization and IRM paleointensity calibration factor f′ and a of the lunar meteorite were calculated to be 3.89 and 3138 μT, respectively, which provides new constraints for the empirical values. The magnetic results of the lunar meteorite demonstrate the utility of our measurement system for small weak samples. This study provides technical support for paleomagnetic study of the Chang’e-5 and other extraterrestrial samples in the future.
Syndromic forms of congenital hyperinsulinism
Authors: Zenker Martin , Mohnike Klaus , Palm Katja
Published: March 2023

Congenital hyperinsulinism (CHI), also called hyperinsulinemic hypoglycemia (HH), is a very heterogeneous condition and represents the most common cause of severe and persistent hypoglycemia in infancy and childhood. The majority of cases in which a genetic cause can be identified have monogenic defects affecting pancreatic β-cells and their glucose-sensing system that regulates insulin secretion. However, CHI/HH has also been observed in a variety of syndromic disorders. The major categories of syndromes that have been found to be associated with CHI include overgrowth syndromes (e.g. Beckwith-Wiedemann and Sotos syndromes), chromosomal and monogenic developmental syndromes with postnatal growth failure (e.g. Turner, Kabuki, and Costello syndromes), congenital disorders of glycosylation, and syndromic channelopathies (e.g. Timothy syndrome). This article reviews syndromic conditions that have been asserted by the literature to be associated with CHI. We assess the evidence of the association, as well as the prevalence of CHI, its possible pathophysiology and its natural course in the respective conditions. In many of the CHI-associated syndromic conditions, the mechanism of dysregulation of glucose-sensing and insulin secretion is not completely understood and not directly related to known CHI genes. Moreover, in most of those syndromes the association seems to be inconsistent and the metabolic disturbance is transient. However, since neonatal hypoglycemia is an early sign of possible compromise in the newborn, which requires immediate diagnostic efforts and intervention, this symptom may be the first to bring a patient to medical attention. As a consequence, HH in a newborn or infant with associated congenital anomalies or additional medical issues remains a differential diagnostic challenge and may require a broad genetic workup.
Nickel oxide nanoparticles exposure as a risk factor for male infertility: “In vitro” effects on porcine pre-pubertal Sertoli cells
Authors: Arato Iva , Giovagnoli Stefano , Di Michele Alessandro , Bellucci Catia , Lilli Cinzia , Aglietti Maria Chiara , Bartolini Desirée , Gambelunghe Angela , Muzi Giacomo , Calvitti Mario , Eugeni Elena , Gaggia Francesco , Baroni Tiziano , Mancuso Francesca , Luca Giovanni
Published: March 2023

Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different industrial and biomedical fields. Several studies have reported that NiO NPs may affect the development of reproductive organs inducing oxidative stress and, resulting in male infertility. We investigated the in vitro effects of NiO NPs on porcine pre-pubertal Sertoli cells (SCs) which undergone acute (24 h) and chronic (from 1 up to 3 weeks) exposure at two subtoxic doses of NiO NPs of 1 μg/ml and 5 μg/ml. After NiO NPs exposure we performed the following analysis: (a) SCs morphological analysis (Light Microscopy); (b) ROS production and oxidative DNA damage, gene expression of antioxidant enzymes (c) SCs functionality (AMH, inhibin B Real-time PCR analysis and ELISA test); (d) apoptosis (WB analysis); (e) pro-inflammatory cytokines (Real-time PCR analysis), and (f) MAPK kinase signaling pathway (WB analysis). We found that the SCs exposed to both subtoxic doses of NiO NPs didn’t sustain substantial morphological changes. NiO NPs exposure, at each concentration, reported a marked increase of intracellular ROS at the third week of treatment and DNA damage at all exposure times. We demonstrated, un up-regulation of SOD and HO-1 gene expression, at both concentrations tested. The both subtoxic doses of NiO NPs detected a down-regulation of AMH and inhibin B gene expression and secreted proteins. Only the 5 μg/ml dose induced the activation of caspase-3 at the third week. At the two subtoxic doses of NiO NPs a clear pro-inflammatory response was resulted in an up-regulation of TNF-α and IL-6 in terms of mRNA. Finally, an increased phosphorylation ratio of p-ERK1/2, p-38 and p-AKT was observed up to the third week, at both concentrations. Our results show the negative impact of subtoxic doses NiO NPs chronic exposure on porcine SCs functionality and viability.
Osteocalcin modulates parathyroid cell function in human parathyroid tumors
Authors: Verdelli Chiara , Tavanti Giulia Stefania , Forno Irene , Vaira Valentina , Maggiore Riccardo , Vicentini Leonardo , Dalino Ciaramella Paolo , Perticone Francesca , Lombardi Giovanni , Corbetta Sabrina
Published: March 2023

Introduction

The bone matrix protein osteocalcin (OC), secreted by osteoblasts, displays endocrine effects. We tested the hypothesis that OC modulates parathyroid tumor cell function.

Methods

Primary cell cultures derived from parathyroid adenomas (PAds) and HEK293 cells transiently transfected with the putative OC receptor GPRC6A or the calcium sensing receptor (CASR) were used as experimental models to investigate γ-carboxylated OC (GlaOC) or uncarboxylated OC (GluOC) modulation of intracellular signaling.

Results

In primary cell cultures derived from PAds, incubation with GlaOC or GluOC modulated intracellular signaling, inhibiting pERK/ERK and increasing active β-catenin levels. GlaOC increased the expression of PTH, CCND1 and CASR, and reduced CDKN1B/p27 and TP73. GluOC stimulated transcription of PTH, and inhibited MEN1 expression. Moreover, GlaOC and GluOC reduced staurosporin-induced caspase 3/7 activity. The putative OC receptor GPRC6A was detected in normal and tumor parathyroids at membrane or cytoplasmic level in cells scattered throughout the parenchyma. In PAds, the membrane expression levels of GPRC6A and its closest homolog CASR positively correlated; GPRC6A protein levels positively correlated with circulating ionized and total calcium, and PTH levels of the patients harboring the analyzed PAds. Using HEK293A transiently transfected with either GPRC6A or CASR, and PAds-derived cells silenced for CASR, we showed that GlaOC and GluOC modulated pERK/ERK and active β-catenin mainly through CASR activation.

Conclusion

Parathyroid gland emerges as a novel target of the bone secreted hormone osteocalcin, which may modulate tumor parathyroid CASR sensitivity and parathyroid cell apoptosis.
Association of BMI with erectile dysfunction: A cross-sectional study of men from an andrology clinic
Authors: Liu Yixun , Hu Xuechun , Xiong Mengneng , Li Jiyan , Jiang Xiaohua , Wan Yangyang , Bai Shun , Zhang Xiansheng
Published: March 2023

Abnormal body mass index (BMI) is associated with an increased risk of erectile dysfunction (ED). However, the relationship between different BMI categories and the levels of ED severity remains unclear. In the current study, 878 men from the andrology clinic in Central China were recruited. Erectile function was assessed by the International Index of Erectile Function (IIEF) scores. Questionnaires included questions about demographic characteristics (age, height, weight, educational status), lifestyle habits (drinking, smoking, sleep time), and medical history. Logistic regression was used to examine the association between ED risk and BMI. The incidence of ED was 53.1%. BMI was significantly higher in men from the ED group than in those from the non-ED group (P = 0.01). Compared with the normal weight group, obese men had a higher risk of ED (OR = 1.97, 95% CI = 1.25-3.14, P = 0.004), even after adjustment for potential confounders (OR = 1.78, 95% CI = 1.10-2.90, P = 0.02). Moreover, the positive correlation between obesity and moderate/severe ED severity was confirmed by logistic regression analysis (moderate/severe ED, OR = 2.71, 95% CI = 1.44-5.04, P = 0.002), even after adjusting for potential confounders (OR = 2.51 95% CI = 1.24-5.09, P = 0.01). Collectively, our findings indicate a positive correlation between obesity and the risk of moderate/severe ED. Clinicians could pay more attention to moderate/severe ED patients to maintain a healthy body weight to improve erectile function.
Ready to fly? Comparing acceptance and behavioral usage intentions of CO2-based aviation fuels in four European countries
Authors: Arning Katrin , Engelmann Linda , Ziefle Martina
Published: March 2023

Significantly increased global greenhouse gas emissions from aviation make the decarbonization of the aviation sector an urgent demand to combat climate change. One technical approach is the usage of Carbon Capture and Utilization technologies (CCU) to re-use CO2 as raw material and to produce CO2-based aviation fuels. As the social readiness is an essential component for a successful roll-out, this study investigates acceptance and behavioral usage intentions regarding CO2-based aviation fuels. We applied an empirical quantitative online questionnaire in four European countries (Spain, Norway, Netherlands, and Germany, N = 2,187). To get a comprehensive overview of the factors that predict social readiness, data on relevant impact factors were collected, including sociodemographic factors, awareness, attitudinal factors (innovation cautiousness, environmental awareness, flight shame), flight behavior as well as evaluations in terms of benefit and risk perceptions of CO2-based fuels. Employing hierarchical regression analyses we identified the impact of individual factors and fuel-related perceptions on the acceptance of and the willingness to use CO2-based aviation fuels. For the prediction of CO2-based fuel acceptance, benefit perceptions were the strongest predictor, followed by environmental awareness, risk perceptions, interest, and flight shame. For the behavioral intention to fly with CO2-based fuels, benefit perceptions showed the strongest impact, followed by environmental awareness, interest, and risk perceptions about technical quality and -maturity as well as health- and environmental risks. This was valid for all four European countries under study, even though there were also national differences: Norwegian respondents showed the lowest interest in and knowledge of CO2-based aviation fuels and the lowest acceptance. Spanish respondents reported the highest acceptance, while acceptance scores of German and Dutch residents ranged in between. Overall, the findings provide a pan-European insight into the social readiness for CO2-based aviation fuels and its determinants, providing targeted information on public adoption conditions and requirements for Carbon Capture and Utilization technology developers and the aviation industry.
A study of the effects of climate change and human activities on NPP of marsh wetland vegetation in the Yellow River source region between 2000 and 2020
Authors: Feng Xueke , Zhao Zhizhong , Ma Tao , Hu Bixia
Published: March 2023

Quantitative assessment of the impacts of climate change and human activities on marsh wetland is essential for the sustainable development of marsh wetland ecosystem. This study takes the marsh wetland in the Yellow River source region (YRSR) as the research object, using the method of residual analysis, the potential net primary productivity (NPPp) of marsh wetland vegetation in the YRSR between 2000 and 2020 was stimulated using the Zhou Guangsheng model, and the actual primary productivity (NPPa) of marsh wetland vegetation was download from MOD17A3HGF product, and the difference between them was employed to calculate the NPP affected by human activities, the relative contribution of climate change and human activities to the change of NPPa of marsh wetland vegetation was quantitatively evaluated. The results revealed that between 2000 and 2020, NPPa of marsh wetland vegetation increased in the YRSR by 95.76%, among which climate-dominated and human-dominated NPP change occupied by 66.29% and 29.47% of study areas, respectively. The Zoige Plateau in the southeast accounted for the majority of the 4.24% decline in the NPPa of the marsh wetland vegetation, almost all of which were affected by human activities. It is found that the warming and humidifying of climate, as well as human protective construction activities, are the important reasons for the increase of NPPa of marsh wetland vegetation in the YRSR. Although climate change remains an important cause of the increase in NPPa of marsh wetland vegetation, the contribution of human activities to the increase in NPPa of marsh wetland vegetation is increasing.
The importance of monsoon precipitation for foundation tree species across the semiarid Southwestern U.S.
Authors: Samuels-Crow Kimberly E. , Peltier Drew M. P. , Liu Yao , Guo Jessica S. , Welker Jeffrey M. , Anderegg William R. L. , Koch George W. , Schwalm Christopher , Litvak Marcy , Shaw John D. , Ogle Kiona
Published: March 2023

Forest dynamics in arid and semiarid regions are sensitive to water availability, which is becoming increasingly scarce as global climate changes. The timing and magnitude of precipitation in the semiarid southwestern U.S. (“Southwest”) has changed since the 21st century began. The region is projected to become hotter and drier as the century proceeds, with implications for carbon storage, pest outbreaks, and wildfire resilience. Our goal was to quantify the importance of summer monsoon precipitation for forested ecosystems across this region. We developed an isotope mixing model in a Bayesian framework to characterize summer (monsoon) precipitation soil water recharge and water use by three foundation tree species (Populus tremuloides [aspen], Pinus edulis [piñon], and Juniperus osteosperma [Utah juniper]). In 2016, soil depths recharged by monsoon precipitation and tree reliance on monsoon moisture varied across the Southwest with clear differences between species. Monsoon precipitation recharged soil at piñon-juniper (PJ) and aspen sites to depths of at least 60 cm. All trees in the study relied primarily on intermediate to deep (10-60 cm) moisture both before and after the onset of the monsoon. Though trees continued to primarily rely on intermediate to deep moisture after the monsoon, all species increased reliance on shallow soil moisture to varying degrees. Aspens increased reliance on shallow soil moisture by 13% to 20%. Utah junipers and co-dominant ñons increased their reliance on shallow soil moisture by about 6% to 12%. Nonetheless, approximately half of the post-monsoon moisture in sampled piñon (38-58%) and juniper (47-53%) stems could be attributed to the monsoon. The monsoon contributed lower amounts to aspen stem water (24-45%) across the study area with the largest impacts at sites with recent precipitation. Therefore, monsoon precipitation is a key driver of growing season moisture that semiarid forests rely on across the Southwest. This monsoon reliance is of critical importance now more than ever as higher global temperatures lead to an increasingly unpredictable and weaker North American Monsoon.
Predicting late-stage age-related macular degeneration by integrating marginally weak SNPs in GWA studies
Authors: Zhou Xueping , Zhang Jipeng , Ding Ying , Huang Heng , Li Yanming , Chen Wei
Published: March 2023

Introduction: Age-related macular degeneration (AMD) is a progressive neurodegenerative disease and the leading cause of blindness in developed countries. Current genome-wide association studies (GWAS) for late-stage age-related macular degeneration are mainly single-marker-based approaches, which investigate one Single-Nucleotide Polymorphism (SNP) at a time and postpone the integration of inter-marker Linkage-disequilibrium (LD) information in the downstream fine mappings. Recent studies showed that directly incorporating inter-marker connection/correlation into variants detection can help discover novel marginally weak single-nucleotide polymorphisms, which are often missed in conventional genome-wide association studies, and can also help improve disease prediction accuracy.

Methods: Single-marker analysis is performed first to detect marginally strong single-nucleotide polymorphisms. Then the whole-genome linkage-disequilibrium spectrum is explored and used to search for high-linkage-disequilibrium connected single-nucleotide polymorphism clusters for each strong single-nucleotide polymorphism detected. Marginally weak single-nucleotide polymorphisms are selected via a joint linear discriminant model with the detected single-nucleotide polymorphism clusters. Prediction is made based on the selected strong and weak single-nucleotide polymorphisms.

Results: Several previously identified late-stage age-related macular degeneration susceptibility genes, for example, BTBD16, C3, CFH, CFHR3, HTARA1, are confirmed. Novel genes DENND1B, PLK5, ARHGAP45, and BAG6 are discovered as marginally weak signals. Overall prediction accuracy of 76.8% and 73.2% was achieved with and without the inclusion of the identified marginally weak signals, respectively.

Conclusion: Marginally weak single-nucleotide polymorphisms, detected from integrating inter-marker linkage-disequilibrium information, may have strong predictive effects on age-related macular degeneration. Detecting and integrating such marginally weak signals can help with a better understanding of the underlying disease-development mechanisms for age-related macular degeneration and more accurate prognostics.
Factors associated with knowledge and vaccination intention for human papillomavirus on trans girls by their main caregiver: A cross-sectional study
Authors: Domínguez-Riscart Jesus , Ariza-Jimenez Ana-Belen , Baez-Castillo Celia , Mateo-Gavira Isabel
Published: March 2023

Introduction

Trans women are highly affected by the human papillomavirus (HPV) and are at risk of suffering from HPV-related diseases such as oropharyngeal, anal, penile, or neovaginal neoplasia. HPV vaccination seems to be a good strategy to reduce HPV-related diseases, mainly during the early age before the first sexual intercourse, but only cisgender girls are covered by the National Health Services, while some high-risk groups such as trans girls are not included. Achieving a high vaccination rate is important in the adolescent population, but there are many factors that could affect it, such as lack of knowledge about HPV or fear of side effects by patients and main caregivers. The aim of our study is to analyze the knowledge of trans girls’ main caregivers about HPV-related diseases in the general population and, in particular, in trans women, as well as factors associated with HPV vaccination intention.

Methods

A cross-sectional study was performed with the collaboration of main caregivers of adolescent trans girls, between 9 and 16 years old, assisted in two reference centers’ multidisciplinary Gender Diversity Units. Information was requested through a self-completed questionnaire: HPV-related diseases Knowledge Transwomen questionnaire (HPV-TQ) was elaborated based on a 19-item self-administered questionnaire and score was standardized from 0 to 19 points. Percentage of correct answers was calculated and defined by the group of high scores that showed over 70% correct answers.

Results

A total of 65 main caregivers were included. Almost all main caregivers were mothers with a Caucasian ethnicity. The HPV-TQ average score was 11 (3.7) with an average correct answer of 58.1% (19.6). Only 17/65 (26.1%) of main caregivers were highly knowledgeable in HPV. Of 65 trans girls, 14 were already vaccinated (29.8% of trans girls over 12 years old); 78.5% were not vaccinated and only 21.5% had intentions to be vaccinated. The group with a high score in HPV-TQ had a longer follow-up at the transgender unit, a higher maternal vaccination rate, and a positive family history of HPV-related disease, especially in mothers.

Conclusion

Adolescent trans girls attended to in our units had a low rate and a low intention of vaccination against HPV. Education on and promotion and prevention of transgender HPV-related diseases should probably be implemented to achieve a higher knowledge and vaccination coverage in adolescent trans girls.
PIK3C2A is a prognostic biomarker that is linked to immune infiltrates in kidney renal clear cell carcinoma
Authors: Qin Chengdong , Liu Siyuan , Zhou Shiqi , Wang Qibo , Xia Xianghou , Hu Jiejie , Yuan Xiaohong , Wang Zongping , Yu Yang , Ma Dening
Published: March 2023

Background

Phosphoinositide 3-kinases (PI3Ks) are lipid enzymes that regulate a wide range of intracellular functions. In contrast to Class I and Class III PI3K, which have more detailed descriptions, Class II PI3K has only recently become the focus of functional research. PIK3C2A is a classical member of the PI3Ks class II. However, the role of PIK3C2A in cancer prognosis and progression remains unknown.

Methods

The expression pattern and prognostic significance of PIK3C2A in human malignancies were investigated using multiple datasets and scRNA-seq data. The PIK3C2A expression in renal clear cell carcinoma (KIRC) was then validated utilizing Western blot. The functional role of PIK3C2A in KIRC was assessed using combined function loss experiments with in vitro experiments. Furthermore, the correlation of PIK3C2A expression with tumor immunity was investigated in KIRC. The TCGA database was employed to investigate PIK3C2A functional networks.

Results

Significant decrease in PIK3C2A expression in KIRC, demonstrated that it potentially influences the prognosis of diverse tumors, particularly KIRC. In addition, PIK3C2A was significantly correlated with the T stage, M stage, pathologic stage, and histologic grade of KIRC. Nomogram models were constructed and used to predict patient survival based on the results of multivariate Cox regression analysis. PIK3C2A knockdown resulted in significantly increased KIRC cell proliferation. Of note, PIK3C2A expression demonstrated a significant correlation with the infiltrating levels of primary immune cells in KIRC.

Conclusion

These findings support the hypothesis that PIK3C2A is a novel biomarker for tumor progression and indicates dynamic shifts in immune infiltration in KIRC. Furthermore, aberrant PIK3C2A expression can influence the biological activity of cancer cells.
Single-cell analyses reveal the dynamic functions of Itgb2+ microglia subclusters at different stages of cerebral ischemia-reperfusion injury in transient middle cerebral occlusion mice model
Authors: Zeng Fanning , Cao Jun , Hong Zexuan , Liu Yujun , Hao Jie , Qin Zaisheng , Zou Xin , Tao Tao
Published: March 2023

Introduction

The underlying pathophysiological mechanisms of cerebral ischemia reperfusion injury (CIRI) is intricate, and current studies suggest that neuron, astrocyte, microglia, endothelial cell, and pericyte all have different phenotypic changes of specific cell types after ischemic stroke. And microglia account for the largest proportion after CIRI. Previous transcriptomic studies of ischemic stroke have typically focused on the 24 hours after CIRI, obscuring the dynamics of cellular subclusters throughout the disease process. Therefore, traditional methods for identifying cell types and their subclusters may not be sufficient to fully unveil the complexity of single-cell transcriptional profile dynamics caused by an ischemic stroke.

Methods

In this study, to explore the dynamic transcriptional profile of single cells after CIRI, we used single-cell State Transition Across-samples of RNA-seq data (scSTAR), a new bioinformatics method, to analyze the single-cell transcriptional profile of day 1, 3, and 7 of transient middle cerebral artery occlusion (tMCAO) mice. Combining our bulk RNA sequences and proteomics data, we found the importance of the integrin beta 2 (Itgb2) gene in post-modeling. And microglia of Itgb2+ and Itgb2- were clustered by the scSTAR method. Finally, the functions of the subpopulations were defined by Matescape, and three different time points after tMCAO were found to exhibit specific functions.

Results

Our analysis revealed a dynamic transcriptional profile of single cells in microglia after tMCAO and explored the important role of Itgb2 contributed to microglia by combined transcriptomics and proteomics analysis after modeling. Our further analysis revealed that the Itgb2+ microglia subcluster was mainly involved in energy metabolism, cell cycle, angiogenesis, neuronal myelin formation, and repair at 1, 3, and 7 days after tMCAO, respectively.

Discussion

Our results suggested that Itgb2+ microglia act as a time-specific multifunctional immunomodulatory subcluster during CIRI, and the underlying mechanisms remain to be further investigated.
Emerging insights into the role of ferroptosis in the pathogenesis of autoimmune diseases
Authors: Fan Junyu , Jiang Ting , He Dongyi
Published: March 2023

Ferroptosis, a novel type of regulated cell death mediated by iron-dependent lipid oxidation, was discovered a decade ago. Significant progress has been made in our knowledge of ferroptosis and immune dysfunction. This review covers recent advancements in the interaction of ferroptosis and the immune system, with an emphasis on autoimmune diseases. The critical regulators of ferroptosis are summarized in the context of reactive oxygen species biology, lipid metabolism, and iron homeostasis. The molecular crosstalk between ferroptosis and different immune cells is also highlighted. Future research is expected to yield new insights into the mechanisms governing ferroptosis and its potential therapeutic benefits in autoimmune diseases.
Acute exercise and high-glucose ingestion elicit dynamic and individualized responses in systemic markers of redox homeostasis
Authors: Thomas Hannah J. , Ang Teddy , Morrison Dale J. , Keske Michelle A. , Parker Lewan
Published: March 2023

Background

Biomarkers of oxidation-reduction (redox) homeostasis are commonly measured in human blood to assess whether certain stimuli (e.g., high-glucose ingestion or acute exercise) lead to a state of oxidative distress (detrimental to health) or oxidative eustress (beneficial to health). Emerging research indicates that redox responses are likely to be highly individualized, yet few studies report individual responses. Furthermore, the effects of complex redox stimuli (e.g., high-glucose-ingestion after exercise) on redox homeostasis remains unclear. We investigated the effect of acute exercise (oxidative eustress), high-glucose ingestion (oxidative distress), and high-glucose ingestion after exercise (both oxidative eu/distress), on commonly measured redox biomarkers in serum/plasma.

Methods

In a randomized crossover fashion, eight healthy men (age: 28 ± 4 years; BMI: 24.5 ± 1.5 kg/m2 [mean ± SD]) completed two separate testing conditions; 1) consumption of a high-glucose mixed-nutrient meal (45% carbohydrate [1.1 g glucose.kg-1], 20% protein, and 35% fat) at rest (control trial), and 2) consumption of the same meal 3 h and 24 h after 1 h of moderate-intensity cycling exercise (exercise trial). Plasma and serum were analyzed for an array of commonly studied redox biomarkers.

Results

Oxidative stress and antioxidant defense markers (hydrogen peroxide, 8-isoprostanes, catalase, superoxide dismutase, and nitrate levels) increased immediately after exercise (p < 0.05), whereas nitric oxide activity and thiobarbituric acid reactive substances (TBARS) remained similar to baseline (p > 0.118). Nitric oxide activity and nitrate levels decreased at 3 h post-exercise compared to pre-exercise baseline levels. Depending on when the high-glucose mixed nutrient meal was ingested and the postprandial timepoint investigated, oxidative stress and antioxidant defense biomarkers either increased (hydrogen peroxide, TBARS, and superoxide dismutase), decreased (hydrogen peroxide, 8-isoprostanes, superoxide dismutase, nitric oxide activity, nitrate, and nitrite), or remained similar to pre-meal baseline levels (hydrogen peroxide, 8-isoprostanes, TBARS, catalase, superoxide dismutase and nitrite). Redox responses exhibited large inter-individual variability in the magnitude and/or direction of responses.

Conclusion

Findings highlight the necessity to interpret redox biomarkers in the context of the individual, biomarker measured, and stimuli observed. Individual redox responsiveness may be of physiological relevance and should be explored as a potential means to inform personalized redox intervention.
Association between C-reactive protein-albumin-lymphocyte (CALLY) index and overall survival in patients with colorectal cancer: From the investigation on nutrition status and clinical outcome of common cancers study
Authors: Yang Ming , Lin Shi-Qi , Liu Xiao-Yue , Tang Meng , Hu Chun-Lei , Wang Zi-Wen , Zhang Qi , Zhang Xi , Song Meng-Meng , Ruan Guo-Tian , Zhang Xiao-Wei , Liu Tong , Xie Hai-Lun , Zhang He-Yang , Liu Chen-An , Zhang Kang-Ping , Li Qin-Qin , Li Xiang-Rui , Ge Yi-Zhong , Liu Yu-Ying , Chen Yue , Zheng Xin , Shi Han-Ping
Published: March 2023

Background

Colorectal cancer (CRC) is among the most common malignant cancers worldwide, and its development is influenced by inflammation, nutrition, and the immune status. Therefore, we combined C-reactive protein (CRP), albumin, and lymphocyte, which could reflect above status, to be the CRP-albumin-lymphocyte (CALLY) index, and evaluated its association with overall survival (OS) in patients with CRC.

Methods

The clinicopathological and laboratory characteristics of 1260 patients with CRC were collected from the Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) study. Cox regression analysis was performed to assess the association between the CALLY index and OS. A nomogram including sex, age, the CALLY index and TNM stage was constructed. The Concordance Index (C-index) was utilized to evaluate the prognostic value of the CALLY index and classical CRC prognostic factors, such as modified Glasgow prognostic score (mGPS), neutrocyte to lymphocyte ratio (NLR), systemic immune inflammation index (SII), and platelet to lymphocyte ratio (PLR), as well as to assess the prognostic value of the nomogram and TNM stage.

Results

Multivariate Cox regression analyses demonstrated that the CALLY index was independently associated with OS in patients with CRC [Hazard ratio (HR) = 0.91, 95% confidence interval (CI) = 0.87-0.95, P<0.001]. The CALLY index showed the highest prognostic value (C-index = 0.666, 95% CI = 0.638-0.694, P<0.001), followed by mGPS, NLR, SII, and PLR. The nomogram demonstrated higher prognostic value (C-index = 0.784, 95% CI = 0.762-0.807, P<0.001) than the TNM stage.

Conclusion

The CALLY index was independently associated with OS in patients with CRC and showed higher prognostic value than classical CRC prognostic factors. The nomogram could provide more accurate prognostic prediction than TNM stage.