bluuebunny/crossref_metadata_2025 huggingface dataset:
Pericentromeric satellite lncRNAs are induced in cancer-associated fibroblasts and regulate their functions in lung tumorigenesis
Authors: Enukashvily Natella I. , Ponomartsev Nikita V. , Ketkar Avanee , Suezov Roman , Chubar Anna V. , Prjibelski Andrey D. , Shafranskaya Daria D. , Elmshäuser Sabrina , Keber Corinna U. , Stefanova Vera N. , Akopov Andrey L. , Klingmüller Ursula , Pfefferle Petra I. , Stiewe Thorsten , Lauth Matthias , Brichkina Anna I.
Published: January 2023

The abnormal tumor microenvironment (TME) often dictates the therapeutic response of cancer to chemo- and immuno-therapy. Aberrant expression of pericentromeric satellite repeats has been reported for epithelial cancers, including lung cancer. However, the transcription of tandemly repetitive elements in stromal cells of the TME has been unappreciated, limiting the optimal use of satellite transcripts as biomarkers or anti-cancer targets. We found that transcription of pericentromeric satellite DNA (satDNA) in mouse and human lung adenocarcinoma was observed in cancer-associated fibroblasts (CAFs). In vivo, lung fibroblasts expressed pericentromeric satellite repeats HS2/HS3 specifically in tumors. In vitro, transcription of satDNA was induced in lung fibroblasts in response to TGFβ, IL1α, matrix stiffness, direct contact with tumor cells and treatment with chemotherapeutic drugs. Single-cell transcriptome analysis of human lung adenocarcinoma confirmed that CAFs were the cell type with the highest number of satellite transcripts. Human HS2/HS3 pericentromeric transcripts were detected in the nucleus, cytoplasm, extracellularly and co-localized with extracellular vesicles in situ in human biopsies and activated fibroblasts in vitro. The transcripts were transmitted into recipient cells and entered their nuclei. Knock-down of satellite transcripts in human lung fibroblasts attenuated cellular senescence and blocked the formation of an inflammatory CAFs phenotype which resulted in the inhibition of their pro-tumorigenic functions. In sum, our data suggest that satellite long non-coding (lnc) RNAs are induced in CAFs, regulate expression of inflammatory genes and can be secreted from the cells, which potentially might present a new element of cell-cell communication in the TME.
CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancer
Authors: Houthuijzen Julia M. , de Bruijn Roebi , van der Burg Eline , Drenth Anne Paulien , Wientjens Ellen , Filipovic Tamara , Bullock Esme , Brambillasca Chiara S. , Pulver Emilia M. , Nieuwland Marja , de Rink Iris , van Diepen Frank , Klarenbeek Sjoerd , Kerkhoven Ron , Brunton Valerie G. , Scheele Colinda L.G.J. , Boelens Mirjam C. , Jonkers Jos
Published: January 2023

Cancer-associated fibroblasts (CAFs) are abundantly present in the microenvironment of virtually all tumors and strongly impact tumor progression. Despite increasing insight into their function and heterogeneity, little is known regarding the origin of CAFs. Understanding the origin of CAF heterogeneity is needed to develop successful CAF-based targeted therapies. Through various transplantation studies in mice, we show that CAFs in both invasive lobular breast cancer and triple-negative breast cancer originate from mammary tissue-resident normal fibroblasts (NFs). Single-cell transcriptomics, in vivo and in vitro studies reveal the transition of CD26+ and CD26- NF populations into inflammatory CAFs (iCAFs) and myofibroblastic CAFs (myCAFs), respectively. Functional co-culture experiments show that CD26+ NFs transition into pro-tumorigenic iCAFs which recruit myeloid cells in a CXCL12-dependent manner and enhance tumor cell invasion via matrix-metalloproteinase (MMP) activity. Together, our data suggest that CD26+ and CD26- NFs transform into distinct CAF subpopulations in mouse models of breast cancer.
Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis
Authors: Fankhaenel Maria , Hashemi Farahnaz S. Golestan , Mourao Larissa , Lucas Emily , Hosawi Manal M. , Skipp Paul , Morin Xavier , Scheele Colinda L.G.J. , Elias Salah
Published: January 2023

Oriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved protein complex formed by the Gαi subunit of heterotrimeric G proteins, the Leucine-Glycine-Asparagine repeat protein (LGN) and the nuclear mitotic apparatus protein. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as an interactor of LGN in mammary epithelial cells. Annexin A1 acts independently of Gαi to instruct the accumulation of LGN and nuclear mitotic apparatus protein at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of Annexin A1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and luminogenesis in three-dimensional cultures of primary mammary epithelial cells. Our findings establish Annexin A1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.
PDGF inhibits BMP2-induced bone healing
Authors: Novak Sanja , Madunic Josip , Shum Laura , Vucetic Milan , Wang Xi , Tanigawa Hitoshi , Ghosh Mallika , Sanjay Archana , Kalajzic Ivo
Published: January 2023

Bone regeneration depends on a pool of bone/cartilage stem/progenitor cells and signaling mechanisms regulating their differentiation. Using in vitro approach, we have shown that PDGF signaling through PDGFRβ inhibits BMP2-induced osteogenesis, and significantly attenuates expression of BMP2 target genes. We evaluated outcomes of treatment with two anabolic agents, PDGF and BMP2 using different bone healing models. Targeted deletion of PDGFRβ in αSMA osteoprogenitors, led to increased callus bone mass, resulting in improved biomechanical properties of fractures. In critical size bone defects BMP2 treatment increased proportion of osteoprogenitors, while the combined treatment of PDGF BB with BMP2 decreased progenitor number at the injury site. BMP2 treatment induced significant bone formation and increased number of osteoblasts, while in contrast combined treatment with PDGF BB decreased osteoblast numbers. This is in vivo study showing that PDGF inhibits BMP2-induced osteogenesis, but inhibiting PDGF signaling early in healing process does not improve BMP2-induced bone healing.
Ion-tunable antiambipolarity in mixed ion–electron conducting polymers enables biorealistic organic electrochemical neurons
Authors: Harikesh Padinhare Cholakkal , Yang Chi-Yuan , Wu Han-Yan , Zhang Silan , Donahue Mary J. , Caravaca April S. , Huang Jun-Da , Olofsson Peder S. , Berggren Magnus , Tu Deyu , Fabiano Simone
Published: January 2023

Biointegrated neuromorphic hardware holds promise for new protocols to record/regulate signalling in biological systems. Making such artificial neural circuits successful requires minimal device/circuit complexity and ion-based operating mechanisms akin to those found in biology. Artificial spiking neurons, based on silicon-based complementary metal-oxide semiconductors or negative differential resistance device circuits, can emulate several neural features but are complicated to fabricate, not biocompatible and lack ion-/chemical-based modulation features. Here we report a biorealistic conductance-based organic electrochemical neuron (c-OECN) using a mixed ion–electron conducting ladder-type polymer with stable ion-tunable antiambipolarity. The latter is used to emulate the activation/inactivation of sodium channels and delayed activation of potassium channels of biological neurons. These c-OECNs can spike at bioplausible frequencies nearing 100 Hz, emulate most critical biological neural features, demonstrate stochastic spiking and enable neurotransmitter-/amino acid-/ion-based spiking modulation, which is then used to stimulate biological nerves in vivo. These combined features are impossible to achieve using previous technologies.
The largest amber-preserved flower revisited
Authors: Sadowski Eva-Maria , Hofmann Christa-Charlotte
Published: January 2023

Amber exquisitely preserves the delicate organs of fossil flowers for millions of years. However, flower inclusions can be rare and usually do not exceed 10 mm in size. Here we report an exceptionally large flower from late Eocene Baltic amber, measuring 28 mm across, which is about three times as large as most floral inclusions. This fossil was described over 150 years ago asStewartia kowalewskii(Theaceae) and has never been revised. The analysis of pollen extracted from the anthers of the flower inclusion, however, revealed strong affinities to Asian species ofSymplocos(Symplocaceae), prompting the new combinationSymplocos kowalewskiicomb. nov. et emend. This fossil represents the first record of Symplocaceae from Baltic amber and supports affinities of its flora to evergreen broadleaved and mixed mesophytic forests of present-day East and Southeast Asia. The rarity of such large-sized flower inclusions is likely due to the size of the resin outpouring and its properties, which might affect the embedding of plant organs.
Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs
Authors: Kawada Ryu , Jonouchi Tatsuya , Kagita Akihiro , Sato Masae , Hotta Akitsu , Sakurai Hidetoshi
Published: January 2023

Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1), resulting in the nuclear MBNL1 aggregation in the DM1 cells. Loss of MBNL1 function is the pivotal mechanism underlying the pathogenesis of DM1. To develop therapeutics for DM1, proper human in vitro models based on the pathologic mechanism of DM1 are required. In this study, we established robust in vitro skeletal muscle cell models of DM1 with patient-derived induced pluripotent stem cells (iPSCs) using the MyoD1-induced system and iPSCs-derived muscle stem cell (iMuSC) differentiation system. Our newly established DM1 models enable simple quantitative evaluation of nuclear MBNL1 aggregation and the downstream splicing defects. Quantitative analyses using the MyoD1-induced myotubes showed that CTGexp-deleted DM1 skeletal myotubes exhibited a reversal of MBNL1-related pathologies, and antisense oligonucleotide treatment recovered these disease phenotypes in the DM1-iPSCs-derived myotubes. Furthermore, iMuSC-derived myotubes exhibited higher maturity than the MyoD1-induced myotubes, which enabled us to recapitulate the SERCA1 splicing defect in the DM1-iMuSC-derived myotubes. Our quantitative and reproducible in vitro models for DM1 established using human iPSCs are promising for drug discovery against DM1.
Effect of metabolic health and obesity on all-cause death and CVD incidence in Korean adults: a retrospective cohort study
Authors: Kim Ye-Seul , Shin Sang-Jun , Kim Yonghwan , Kim Joungyoun , Kang Hee-Taik
Published: January 2023

This study aimed to investigate the risk of all-cause mortality and incidence of CVD according to metabolic health and body mass index (BMI) in Korean adults. This study was retrospectively designed using the National Health Insurance Service-National Health Screening Cohort data. Participants were divided into six groups according to two category of metabolic syndrome and three categories of BMI. Hazard ratios (HRs) and 95% confidence intervals (CIs) for the composite outcome (all-cause mortality and incidence of CVDs) were estimated using multivariable Cox proportional hazards regression models. 151,706 participants aged ≥ 40 years were enrolled; median follow-up period was 9.7 years in the study. Compared to metabolically healthy normal weight, the fully adjusted HRs (95% CIs) of metabolically healthy overweight, metabolically healthy obese, metabolically unhealthy normal weight, metabolically unhealthy overweight, and metabolically unhealthy obese for composite outcome were 1.07 (1.03–1.12), 1.12 (1.07–1.17), 1.33 (1.25–1.41), 1.28 (1.22–1.34), and 1.31 (1.26–1.37), respectively, in men, and 1.10 (1.05–1.16), 1.22 (1.16–1.29), 1.34 (1.26–1.43), 1.27 (1.19–1.34), and, 1.40 (1.34–1.47), respectively, in women. High BMI and metabolic unhealthiness were associated with an increased risk on the composite of all-cause mortality and incidence of CVD in both sexes.
Breast cancer patients from the Midwest region of the United States have reduced levels of short-chain fatty acid-producing gut bacteria
Authors: Shrode Rachel L. , Knobbe Jessica E. , Cady Nicole , Yadav Meeta , Hoang Jemmie , Cherwin Catherine , Curry Melissa , Garje Rohan , Vikas Praveen , Sugg Sonia , Phadke Sneha , Filardo Edward , Mangalam Ashutosh K.
Published: January 2023

As geographical location can impact the gut microbiome, it is important to study region-specific microbiome signatures of various diseases. Therefore, we profiled the gut microbiome of breast cancer (BC) patients of the Midwestern region of the United States. The bacterial component of the gut microbiome was profiled utilizing 16S ribosomal RNA sequencing. Additionally, a gene pathway analysis was performed to assess the functional capabilities of the bacterial microbiome. Alpha diversity was not significantly different between BC and healthy controls (HC), however beta diversity revealed distinct clustering between the two groups at the species and genera level. Wilcoxon Rank Sum test revealed modulation of several gut bacteria in BC specifically reduced abundance of those linked with beneficial effects such as Faecalibacterium prausnitzii. Machine learning analysis confirmed the significance of several of the modulated bacteria found by the univariate analysis. The functional analysis showed a decreased abundance of SCFA (propionate) production in BC compared to HC. In conclusion, we observed gut dysbiosis in BC with the depletion of SCFA-producing gut bacteria suggesting their role in the pathobiology of breast cancer. Mechanistic understanding of gut bacterial dysbiosis in breast cancer could lead to refined prevention and treatment.
TRAIL mediated apoptosis ruling and anticancer trigger by fine-tuned nano spheres of Fagonia cretica methanolic extracts as novel cancer regime
Authors: Ahmed Warda , Mansoor Qaisar , Ahmad Muhammad Sheeraz , Zainab Tayyaba , Shah Muhammad Ali
Published: January 2023

Fagonia cretica L. is a tropical plant of family Zygophyllaceae with wide range of medicinally important secondary metabolites. The low cellular uptake of the polar compounds in the extract of the plant limits its biological application. In present study efficacy of F. cretica modified bioactive nano-formulations for in vitro modulation of TRAIL mediated extrinsic apoptotic pathway as cancer therapy was investigated. F. cretica methanolic extracts were formulated at nano-scale for green synthesis of silver nanoparticles, albumin conjugation and liposomes encapsulation to enhance targeted bioactivity against cancer. Physical characterization of the synthesized nanoparticles was done by SEM, EDX and Zeta potential analyzer. In vitro cell viability assay MTT was done for MCF-7, Hep-2, HUH-7 and HCEC cell lines. Relative expression variation of the apoptotic pathway-associated genes was done by qRT-PCR. SEM revealed spherical shape of 56.62 ± 8.04, 143 ± 11.54 and 83.36 ± 38.73 nm size and zeta potential − 18.6, − 15.5 and − 18.3 mV for liposomes, silver and albumin nanoparticles. Silver nanoparticles showed highest anticancer activity in vitro than albumin and liposomes nanoparticles with IC50 0.101 ± 0.004, 0.177 ± 0.03 and 0.434 ± 0.022 mg/mL in MCF-7, Hep-2 and HUH-7 respectively. F. cretica albumin and silver nanoparticles upregulated the in vitro TRAIL, DR4, DR5 and FADD gene expression at statistically significant levels in Hep-2 cell lines. Nano-formulations of F. cretica proved therapeutically important biomolecules in vitro. The hypothesized modulation of extrinsic apoptosis pathway genes through the plant nanoparticles proved novel medicinal options for effective treatment of cancer and enhancing the bioavailability of the active plant metabolites.
A novel pH sensitive theranostic PLGA nanoparticle for boron neutron capture therapy in mesothelioma treatment
Authors: Sforzi Jacopo , Lanfranco Alberto , Stefania Rachele , Alberti Diego , Bitonto Valeria , Parisotto Stefano , Renzi Polyssena , Protti Nicoletta , Altieri Saverio , Deagostino Annamaria , Geninatti Crich Simonetta
Published: January 2023

This study aims to develop poly lactic-co-glycolic acid (PLGA) nanoparticles with an innovative imaging-guided approach based on Boron Neutron Capture Therapy for the treatment of mesothelioma. The herein-reported results demonstrate that PLGA nanoparticles incorporating oligo-histidine chains and the dual Gd/B theranostic agent AT101 can successfully be exploited to deliver a therapeutic dose of boron to mesothelioma cells, significantly higher than in healthy mesothelial cells as assessed by ICP-MS and MRI. The selective release is pH responsive taking advantage of the slightly acidic pH of the tumour extracellular environment and triggered by the protonation of imidazole groups of histidine. After irradiation with thermal neutrons, tumoral and healthy cells survival and clonogenic ability were evaluated. Obtained results appear very promising, providing patients affected by this rare disease with an improved therapeutic option, exploiting PLGA nanoparticles.
Microdeletions and microduplications linked to severe congenital disorders in infertile men
Authors: Kikas Triin , Punab Anna Maria , Kasak Laura , Poolamets Olev , Vihljajev Vladimir , Pomm Kristjan , Reiman Mario , Tjagur Stanislav , Korrovits Paul , Punab Margus , Laan Maris
Published: January 2023

Data on the clinical validity of DNA copy number variants (CNVs) in spermatogenic failure (SPGF) is limited. This study analyzed the genome-wide CNV profile in 215 men with idiopathic SPGF and 62 normozoospermic fertile men, recruited at the Andrology Clinic, Tartu University Hospital, Estonia. A two-fold higher representation of > 1 Mb CNVs was observed in men with SPGF (13%, n = 28) compared to controls (6.5%, n = 4). Seven patients with SPGF were identified as carriers of microdeletions (1q21.1; 2.4 Mb) or microduplications (3p26.3, 1.1 Mb; 7p22.3-p22.2, 1.56 Mb; 10q11.22, 1.42 Mb, three cases; Xp22.33; 2.3 Mb) linked to severe congenital conditions. Large autosomal CNV carriers had oligozoospermia, reduced or low-normal bitesticular volume (22–28 ml). The 7p22.3-p22.2 microduplication carrier presented mild intellectual disability, neuropsychiatric problems, and short stature. The Xp22.33 duplication at the PAR1/non-PAR boundary, previously linked to uterine agenesis, was detected in a patient with non-obstructive azoospermia. A novel recurrent intragenic deletion in testis-specific LRRC69 was significantly overrepresented in patients with SPGF compared to the general population (3.3% vs. 0.85%; χ2 test, OR = 3.9 [95% CI 1.8–8.4], P = 0.0001). Assessment of clinically valid CNVs in patients with SPGF will improve their management and counselling for general and reproductive health, including risk of miscarriage and congenital disorders in future offspring.
Assessing the external validity of machine learning-based detection of glaucoma
Authors: Li Chi , Chua Jacqueline , Schwarzhans Florian , Husain Rahat , Girard Michaël J. A. , Majithia Shivani , Tham Yih-Chung , Cheng Ching-Yu , Aung Tin , Fischer Georg , Vass Clemens , Bujor Inna , Kwoh Chee Keong , Popa-Cherecheanu Alina , Schmetterer Leopold , Wong Damon
Published: January 2023

Studies using machine learning (ML) approaches have reported high diagnostic accuracies for glaucoma detection. However, none assessed model performance across ethnicities. The aim of the study is to externally validate ML models for glaucoma detection from optical coherence tomography (OCT) data. We performed a prospective, cross-sectional study, where 514 Asians (257 glaucoma/257 controls) were enrolled to construct ML models for glaucoma detection, which was then tested on 356 Asians (183 glaucoma/173 controls) and 138 Caucasians (57 glaucoma/81 controls). We used the retinal nerve fibre layer (RNFL) thickness values produced by the compensation model, which is a multiple regression model fitted on healthy subjects that corrects the RNFL profile for anatomical factors and the original OCT data (measured) to build two classifiers, respectively. Both the ML models (area under the receiver operating [AUC] = 0.96 and accuracy = 92%) outperformed the measured data (AUC = 0.93; P < 0.001) for glaucoma detection in the Asian dataset. However, in the Caucasian dataset, the ML model trained with compensated data (AUC = 0.93 and accuracy = 84%) outperformed the ML model trained with original data (AUC = 0.83 and accuracy = 79%; P < 0.001) and measured data (AUC = 0.82; P < 0.001) for glaucoma detection. The performance with the ML model trained on measured data showed poor reproducibility across different datasets, whereas the performance of the compensated data was maintained. Care must be taken when ML models are applied to patient cohorts of different ethnicities.
PDJ amplicon in triple negative breast cancer
Authors: Roesler Alexander S. , Malasi Smriti , Koslosky Lori , Hartmayer Peter , Naab Tammey J. , Carter Jodi M. , Zahrieh David , Hillman David , Leon-Ferre Roberto A. , Couch Fergus J. , Goetz Matthew P. , Anderson Karen S. , Pockaj Barbara A. , Barrett Michael T.
Published: January 2023

Amplification of chromosome 9p24.1 targetingPD-L1,PD-L2, andJAK2(PDJ amplicon) is present in subsets of triple negative breast cancers (TNBCs) and is associated with poor clinical outcomes. However, the prevalence of PDJ+ TNBCs varies extensively across studies applying different methods for interrogating samples of interest. To rigorously assess the prevalence of PDJ amplicons in TNBC, its prognostic value and whether it is enriched by chemotherapy, we interrogated 360 TNBC samples including 74 surgical resections from patients treated in the neoadjuvant setting, and tissue microarrays (TMAs) with 31 cases from African American women and 255 resected non-metastatic cases, with a 3 color fluorescence in situ hybridization (FISH) assay targeting the 9p24.1 PDJ amplicon, 9q24.3, and 9q34.1. Samples with mean PDJ signal of > 4.5 copies, and ratios of PDJ/9q24 ≥ 2 and/or PDJ/9q34.1 ≥ 2 were called amplified (PDJ+). Correlative analyses included the association of tumor infiltrating lymphocytes (TILs) with PDJ amplicons in TNBCs. In addition, we investigated intratumor copy number of PDJ amplicons in PDJ+ and PDJ− TNBCs. Matched pre- and post-neoadjuvant treatment biopsies were available from patients (n = 6) to evaluate the effects of therapy on PDJ status. Our study provides a rigorous analysis of the prevalence, distribution, and clinical correlatives of the PDJ amplicon in TNBC.
A novel path towards limiting non-exhaust particulate matter emissions of a commercial friction material through the addition of metallurgical slag
Authors: Jayashree Priyadarshini , Matějka Vlastimil , Leonardi Mara , Straffelini Giovanni
Published: January 2023

Keeping recycling and the circular economy in mind, this study explores the reduction in emission of a highly optimized, commercially employed friction material formulation through the addition of metallurgical slags from a basic oxygen furnace in varying quantities from 6 to 38 wt%. The various compositions were paired with a pearlitic grey cast iron counterface and tested on a pin on disc tribometer. The friction coefficient and pin wear increased with the slag addition but were still within the permissible limit when compared to the original formulation. Specimens with higher slag content observed extremely compacted and extended secondary contact plateaus, which also recorded significant slag presence. The extended plateaus detached in the form of chunks from the mating surfaces, which settled on the equipment hardware and restricted the production of airborne particles. From an industrial symbiosis perspective, the addition of metallurgical slags proved to be a promising way of obtaining green friction materials with reduced emissions.
Changes within the central stalk of E. coli F1Fo ATP synthase observed after addition of ATP
Authors: Sobti Meghna , Zeng Yi C. , Walshe James L. , Brown Simon H. J. , Ishmukhametov Robert , Stewart Alastair G.
Published: January 2023

F1Fo ATP synthase functions as a biological generator and makes a major contribution to cellular energy production. Proton flow generates rotation in the Fo motor that is transferred to the F1 motor to catalyze ATP production, with flexible F1/Fo coupling required for efficient catalysis. F1Fo ATP synthase can also operate in reverse, hydrolyzing ATP and pumping protons, and in bacteria this function can be regulated by an inhibitory ε subunit. Here we present cryo-EM data showing E. coli F1Fo ATP synthase in different rotational and inhibited sub-states, observed following incubation with 10 mM MgATP. Our structures demonstrate how structural transitions within the inhibitory ε subunit induce torsional movement in the central stalk, thereby enabling its rotation within the Fο motor. This highlights the importance of the central rotor for flexible coupling of the F1 and Fo motors and provides further insight into the regulatory mechanism mediated by subunit ε.
In situutilization of photogenerated hydrogen for hydrogenation reaction over a covalent organic framework
Authors: Wang Guang-Bo , Xie Ke-Hui , Kan Jing-Lan , Xu Hai-Peng , Zhao Fei , Wang Yan-Jing , Geng Yan , Dong Yu-Bin
Published: January 2023

Thein situhydrogenation of nitroarenes under visible-light irradiation without any additional hydrogen source, following the photocatalytic hydrogen evolution photocatalyzed by a sp2-carbon conjugated COF, was successfully accomplished.
Photothermal steam reforming of methane over silica-supported nickel catalysts with temperature gradients
Authors: Sarwana Wirya , Takami Daichi , Yamamoto Akira , Yoshida Hisao
Published: January 2023

The present work demonstrated that the photo-formed temperature gradients changed the product selectivity in the photothermal steam reforming of methane.
Inorganic CsPbI2Br halide perovskites: from fundamentals to solar cell optimizations
Authors: Lim Eng Liang , Yang Jinxin , Wei Zhanhua
Published: January 2023

This review provides fundamental knowledge of inorganic CsPbI2Br perovskites and up-to-date/cutting-edge knowledge in solar cell applications.
Bioactive polysaccharides promote gut immunity via different ways
Authors: Wu Xincheng , Huang Xiaojun , Ma Wanning , Li Mingzhi , Wen Jiajia , Chen Chunhua , Liu Liandi , Nie Shaoping
Published: January 2023

Our study revealed that different polysaccharides promote gut immunity in different ways and provided a guideline for the development of superior intestinal immunomodulatory polysaccharides.
Ion transport on self-assembled block copolymer electrolytes with different side chain chemistries
Authors: Ramos-Garcés Mario V. , Senadheera Dodangodage Ishara , Arunagiri Karthik , Angelopoulou Polyxeni P. , Sakellariou Georgios , Li Ke , Vogt Bryan D. , Kumar Revati , Arges Christopher G.
Published: January 2023

Hydrophobic alkyl side chains steer water toward the charge-ion pair, giving rise to large interconnected water clusters that promote ion conduction.
Novel triphenylphosphonium amphiphilic conjugates of glycerolipid type: synthesis, cytotoxic and antibacterial activity, and targeted cancer cell delivery
Authors: Tsepaeva Olga V. , Nemtarev Andrey V. , Pashirova Tatiana N. , Khokhlachev Michail V. , Lyubina Anna P. , Amerkhanova Syumbelya K. , Voloshina Alexandra D. , Mironov Vladimir F.
Published: January 2023

We developed new mitochondriotropic compounds and drug delivery systems with a high cytotoxic effect toward HuTu-80 cells (IC50 0.1 μM), providing a significant selectivity (SI = 480) compared to the normal cells.
Non-equilibrium kinetics for improving ionic conductivity in garnet solid electrolyte
Authors: Wang Youwei , Wang Tiantian , Zhao Xiaolin , Liu Jianjun
Published: January 2023

The non-equilibrium configuration of [LiO6]-[LiO4]-[VLiO6] in Ta5+-doped Li7La3Zr2O12 reduces the activation energy of Li+-ion transport and improves the ionic conductivity.
Measurements of single-molecule electromechanical properties based on atomic force microscopy fixed-junction technique
Authors: Yu Lei , Zhang Mingyang , Chen Haijian , Xiao Bohuai , Chang Shuai
Published: January 2023

A fixed nanogap between electrodes is pre-set and the simultaneous electrical and mechanical properties of a single molecule are obtained from the time-resolved changes in both the current and force induced by stochastic metal–molecule binding.
Temperature-responsive and biocompatible nanocarriers based on clay nanotubes for controlled anti-cancer drug release
Authors: Hemmatpour Hamoon , Haddadi-Asl Vahid , Burgers Thomas C. Q. , Yan Feng , Stuart Marc C. A. , Reker-Smit Catharina , Vlijm Rifka , Salvati Anna , Rudolf Petra
Published: January 2023

Halloysite nanotubes decorated with poly(N-isopropylacrylamide) brushes exhibit temperature-responsive drug release behavior and accumulate in the lysosomes of HeLa cells.
In situfabrication of MIL-68(In)@ZnIn2S4heterojunction for enhanced photocatalytic hydrogen production
Authors: Tan Mengxi , Yu Chengye , Zeng Hua , Liu Chuanbao , Dong Wenjun , Meng Huimin , Su Yanjing , Qiao Lijie , Gao Lei , Lu Qipeng , Bai Yang
Published: January 2023

The type II heterojunction MIL-68(In)@ZIS with hierarchical loose structure is successfully synthesized byin-situgrowth. The optimized photocatalyst shows an excellent photocatalytic performance of 9.09 mmol g−1h−1 with great stability.
Total synthesis of haploscleridamine, villagorgin A and an approach towards lissoclin C
Authors: Roy Moumita Singha , Meng Xiaofeng , Koda Karuna , Shrestha Andrina , Putman Joshua I , Gout Delphine , Armstrong Daniel W. , Lovely Carl J.
Published: January 2023

Total syntheses of scalemic samples of the marine-derived indole alkaloids haploscleridamine and villagorgin A were accomplished from S-histidine relying on ring-closing metathesis reaction and a modified Fischer indole synthesis.
Synthesis of N-vinyl isothiocyanates and carbamates by the cleavage of NH-1,2,3-triazoles with one-carbon electrophiles
Authors: Motornov Vladimir , Beier Petr
Published: January 2023

Metal-free cascade reaction of NH-1,2,3-triazoles with one-carbon electrophiles, such as thiophosgene and triphosgene, led to N-vinylated ring cleavage products.
Xanthene-based functional dyes: towards new molecules operating in the near-infrared region
Authors: Kamino Shinichiro , Uchiyama Masanobu
Published: January 2023

This review article summarizes the recent advances in xanthene-based dyes operating in the far-red and near-infrared wavelength regions.
Halogen bonding between metal-bound I3− and unbound I2: the trapped I2⋯I3− intermediate in the controlled assembly of copper(i)-based polyiodides
Authors: Kinzhalov Mikhail A. , Ivanov Daniil M. , Shishkina Anastasia V. , Melekhova Anna A. , Suslonov Vitalii V. , Frontera Antonio , Kukushkin Vadim Yu. , Bokach Nadezhda A.
Published: January 2023

The identification of halogen bonded-intermediates is key to understanding the precise mechanism for the generation of I5− (and I82−) ligands from I2 and metal-coordinated I3−.
Additive free, N-heterocyclic nitrenium catalyzed photoreduction of cycloketone oxime esters
Authors: Su Xiao-Di , Yang Zhu-Sheng , Gong Wei , Wang Zhi-Xiang , Chen Xiang-Yu
Published: January 2023

N-Heterocyclic nitrenium iodide salts can be used as ideal charge transfer complex catalysts for the photoreduction of cycloketone oxime esters.
Novel electrospun fibers as carriers for delivering a biocompatible Sm(iii) nanodrug for cancer therapy: fabrication, characterization, cytotoxicity and toxicity
Authors: Fouad R. , Ali Amira A. M.
Published: January 2023

The current study represents the successful fabrication and characterization of a Sm(iii) nano complex based on 2-cyano-N′-((4-oxo-4H-chromen-3-yl)methylene)acetohydrazide (CCMA).
A chemically modified solid-state sensor for magnesium(ii) ions and esomeprazole magnesium potentiometric assay
Authors: Saad Ahmed S. , Ismail Nahla S. , Gaber Noran S. , Elzanfaly Eman S.
Published: January 2023

The use of electrochemical sensors offers a simple, affordable solution with great reliability.
N-Cyano sulfilimine functional group as a nonclassical amide bond bioisostere in the design of a potent analogue to anthranilic diamide insecticide
Authors: Kang On-Yu , Kim Eunsil , Lee Won Hyung , Ryu Do Hyun , Lim Hwan Jung , Park Seong Jun
Published: January 2023

To explore the potential of the N-cyano sulfilimine group as an amide bond isostere, a derivative of anthranilic diamide was synthesized and evaluated with regard to its physicochemical properties, permeability, and biological activity.
Novel A-π-D-π-A type non-fullerene acceptors of dithienyl diketopyrropopyrrole derivatives to enhance organic photovoltaic applications: a DFT study
Authors: Rani Mafia , Hadia N. M. A. , Shawky Ahmed M. , Mehmood Rana Farhat , Hameed Shanza , Zahid Saba , Iqbal Javed , Alatawi Naifa S. , Ahmed Asma , Khera Rasheed Ahmad
Published: January 2023

The investigated non-fullerene acceptor molecules TM1, TM2, TM3, TM4, TM5, TM6, and TM7 that are fashioned by making alterations at the terminal position of reference molecule TMR demonstrate significant absorption in the visible region.
A molecular dynamics study on adsorption mechanisms of polar, cationic, and anionic polymers on montmorillonite
Authors: Qu Yuanzhi , Lin Ling , Gao Shifeng , Yang Yukun , Huang Hongjun , Li Xin , Ren Han , Luo Wenjia
Published: January 2023

Adsorption mechanisms and desorption kinetics of anionic, cationic, and nonionic polymers on montmorillonite clay under different temperature, pressure, and salinity conditions are revealed by molecular dynamics simulations.
Cationic styryl dyes for DNA labelling and selectivity toward cancer cells and Gram-negative bacteria
Authors: Wangngae Sirilak , Ngivprom Utumporn , Khrootkaew Tunyawat , Worakaensai Suphanida , Lai Rung-Yi , Kamkaew Anyanee
Published: January 2023

Fluorescence-based techniques are essential for the analysis of nucleic acids. Two readily obtainable small cationic dyes with increased fluorescence following non-covalent DNA binding showed selectivity for Gram-negative bacteria and cancer cells.
Effects of CO2 atmosphere on low-rank coal pyrolysis based on ReaxFF molecular dynamics
Authors: Gu Chenkai , Jin Jing , Li Ye , Li Ruiyang , Dong Bo
Published: January 2023

Pyrolysis of low-rank coal in CO2 atmosphere can reduce carbon emissions while improving the yield and quality of pyrolysis tar.
Surface reconstruction on Ni2P@CC to form an ultrathin layer of Ni(OH)2for enhancing the capture and catalytic conversion of polysulfides in lithium–sulfur batteries
Authors: Peng Lin , Qu Meixiu , Sun Rui , Yang Weiwei , Wang Zhenhua , Sun Wang , Bai Yu
Published: January 2023

An ultrathin layer of Ni(OH)2isin situintroduced to the Ni2P surface by an electrochemical method to realize the regulation of surface properties of Ni2P. The S/Ni(OH)2–Ni2P@CC based cell exhibits superior cycle stability and high areal capacity.
Revisiting carboxylic group functionalization of silica sol–gel materials
Authors: Rivas M. Verónica , Arenas Muñetón María J. , Bordoni Andrea V. , Lombardo M. Verónica , Spagnuolo Carla C. , Wolosiuk Alejandro
Published: January 2023

In this review we present characterization and synthetic chemistry guidelines for obtaining COOH-based alkoxysilanes aiming at SiO2 modification. Applications of these derivatized materials related to biology and medicine are also discussed.
Color-tuning and boosting circularly polarized luminescence performance of axially chiral tetra-BF2complexes by post-modifications
Authors: Cui Luxia , Deyama Koichi , Ichiki Takafumi , Konishi Yuto , Horioka Ami , Harada Takunori , Ishibashi Kohei , Hisaeda Yoshio , Ono Toshikazu
Published: January 2023

Multicolored fluorescence, high ΦPLreaching 59%, and an efficientglumup to 0.01 with the brightness for circularly polarized luminescence (125.2 M−1 cm−1), were achieved in axially chiral tetra-BF2 complexes by substituent engineering.
Temporal Trends in Clinician-Assessed and Patient-Reported Swallowing Outcomes in Oropharyngeal Cancer Patients
Authors: Kabalan Sarah , Papadopoulos-Nydam Georgina , Jeffery Caroline C. , Rieger Jana , Constantinescu Gabriela
Published: January 2023

Background:

Dysphagia is a consequence of oropharyngeal squamous cell carcinoma (OPSCC) treatments and often results in a devastating reduction in quality of life (QoL; Nguyen et al., 2005; Pauloski, 2008).

Objectives:

This study aimed to report temporal trends in swallowing outcomes using the Modified Barium Swallow Impairment Profile (MBSImP) and the M.D. Anderson Dysphagia Inventory (MDADI) and to study the relationship between these two measures.

Method:

This was a retrospective review of clinical data collected in January 2013 to December 2017 from a tertiary care center. MBSImP PI scores and MDADI composite scores were collected pretreatment and 1, 6, and 12 months posttreatment. Data were analyzed in aggregate and stratified by treatment modality. To address the primary objective, descriptive statistics were used. To address the secondary objective, four Spearman tests were run between MBSImP PI and MDADI composite scores.

Results:

A total of 123 OPSCC participants were included. With respect to trends, MBSImP PI scores worsened 1 month posttreatment and remained impaired at 6 and 12 months. For MDADI composite scores, patient reports worsened 1 month posttreatment and subsequently improved at 6 and 12 months. MBSImP PI and MDADI composite scores were weakly negatively correlated (i.e., in agreement) at the pre- and 12-month posttreatment appointments.

Conclusions:

Swallowing outcomes in OPSCC patients have distinct yet predictable trends for both clinician-assessed and patient-reported swallowing outcomes during the first year following cancer treatment. However, unlike previous findings, these two types of measures were in agreement at the pretreatment and 12 months posttreatment appointments.
Retracted: Track and field competition data collection using a vision sensor system and machine learning
Authors: Meng Yan , Wu Yunming
Published: January 2023

Retraction: [Yan Meng, Yunming Wu, Track and field competition data collection using a vision sensor system and machine learning, IET Software 2023 (https://doi.org/10.1049/sfw2.12086)].

The above article from IET Software, published online on 11 January 2023 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the Editor‐in‐Chief, Hana Chockler, the Institution of Engineering and Technology (the IET) and John Wiley and Sons Ltd. This article was published as part of a Guest Edited special issue. Following an investigation, the IET and the journal have determined that the article was not reviewed in line with the journal's peer review standards and there is evidence that the peer review process of the special issue underwent systematic manipulation. Accordingly, we cannot vouch for the integrity or reliability of the content. As such we have taken the decision to retract the article. The authors have been informed of the decision to retract.
Spiral-like features in the disc revealed by Gaia DR3 radial actions
Authors: Palicio P. A. , Recio-Blanco A. , Poggio E. , Antoja T. , McMillan P. J. , Spitoni E.
Published: January 2023

Context. The so-called action variables are specific functions of the positions and velocities that remain constant along the stellar orbit. The astrometry provided by Gaia Early Data Release 3 (EDR3), combined with the velocities inferred from the Radial Velocity Spectrograph (RVS) spectra of Gaia DR3, allows for the estimation of these actions for the largest volume of stars to date.

Aims. We explore such actions with the aim of locating structures in the Galactic disc.

Methods. We computed the actions and the orbital parameters of the Gaia DR3 stars, assuming an axisymmetric model for the Milky Way. Using Gaia DR3 photometric data, we also selected a subset of giant stars with better astrometry as a control sample.

Results. We find that the maps of the percentiles of the radial action JR reveal arc-like segments. We found a high JR region centered at R ≈ 10.5 kpc of 1 kpc width, as well as three arc-shape regions dominated by circular orbits at inner radii. We also identified the spiral arms in the overdensities of the giant population.

Conclusions. For Galactic coordinates (X, Y, Z), we find good agreement with the literature in the innermost region for the Scutum-Sagittarius spiral arms. At larger radii, the low JR structure tracks the Local arm at negative X, while for the Perseus arm, the agreement is restricted to the X <  2 kpc region, with a displacement with respect to the literature at more negative longitudes. We detected a high JR area at a Galactocentric radii of ∼10.5 kpc, consistent with some estimations of the Outer Lindblad Resonance location. We conclude that the pattern in the dynamics of the old stars is consistent in several places with the spatial distribution of the spiral arms traced by young populations, with small potential contributions from the moving groups.
The CARMENES search for exoplanets around M dwarfs
Authors: Blanco-Pozo J. , Perger M. , Damasso M. , Anglada Escudé G. , Ribas I. , Baroch D. , Caballero J. A. , Cifuentes C. , Jeffers S. V. , Lafarga M. , Kaminski A. , Kaur S. , Nagel E. , Perdelwitz V. , Pérez-Torres M. , Sozzetti A. , Viganò D. , Amado P. J. , Andreuzzi G. , Béjar V. J. S. , Brown E. L. , Del Sordo F. , Dreizler S. , Galadí-Enríquez D. , Hatzes A. P. , Kürster M. , Lanza A. F. , Melis A. , Molinari E. , Montes D. , Murgia M. , Pallé E. , Peña-Moñino L. , Perrodin D. , Pilia M. , Poretti E. , Quirrenbach A. , Reiners A. , Schweitzer A. , Zapatero Osorio M. R. , Zechmeister M.
Published: January 2023

Context. Detecting a planetary companion in a short-period orbit through radio emission from the interaction with its host star is a new prospect in exoplanet science. Recently, a tantalising signal was found close to the low-mass stellar system GJ 1151 using LOFAR observations.

Aims. We studied spectroscopic time-series data of GJ 1151 in order to search for planetary companions, investigate possible signatures of stellar magnetic activity, and to find possible explanations for the radio signal.

Methods. We used the combined radial velocities measured from spectra acquired with the CARMENES, HARPS-N, and HPF instruments, extracted activity indices from those spectra in order to mitigate the impact of stellar magnetic activity on the data, and performed a detailed analysis of Gaia astrometry and all available photometric time series coming from the MEarth and ASAS-SN surveys.

Results. We found a M > 10.6 M⊕ companion to GJ 1151 in a 390d orbit at a separation of 0.57 au. Evidence for a second modulation is also present; this could be due to long-term magnetic variability or a second (substellar) companion. The star shows episodes of elevated magnetic activity, one of which could be linked to the observed LOFAR radio emission. We show that it is highly unlikely that the detected GJ 1151 c, or any additional outer companion is the source of the detected signal. We cannot firmly rule out the suggested explanation of an undetected short-period planet that could be related to the radio emission, as we establish an upper limit of 1.2 M⊕ for the minimum mass.
Reflection on the Communication of Urban Image of Chaoshan Area in the Era of New Media
Authors: Fang Huan , Zhang Tian , Zhang Yanting
Published: January 2023

Since the “13th Five-Year Plan”, China has made remarkable achievements in the construction of new urbanization, and its development has also shifted from increment and expansion to a new stage dominated by stock renewal. In the tide of diversified construction of city, Chaoshan area with strong regional characteristics and cultural deposit has been frequently frustrated in shaping its urban image. Many untrue public opinions and rumors even appear on new media platforms and are widespread. How to effectively communicate the urban brand image of Chaoshan area and therefore promote regional economic development has become an aspiration of every Chaoshan person. In this paper, by collecting and analyzing questionnaires, we design a “CBIO” model. In order to improve communication effect of urban image of Chaoshan area, communication subjects should paid attention to key factors such as communication tactics, urban brand positioning and urban infrastructure construction.
On Strengthening the Dissemination of Positive Chinese Traditional Culture in Colleges and Universities in the New Media Era
Authors: Li Kexin
Published: January 2023

General Secretary Xi Jinping once profoundly pointed out that “Positive Chinese traditional culture is the root and soul of the everlasting Chinese nation”.1 However, the traditional communication mode of positive Chinese traditional culture is facing challenges and limitations in colleges and universities in the new media era. The development of a comprehensive new media platform is an inevitable trend to strengthen the dissemination of positive Chinese traditional culture in colleges and universities. The coexistence and collision of traditional media and new media has brought opportunities and challenges to the spread of positive Chinese traditional culture. To attract young audiences, mainly college students, it is necessary to expand the effective communication paths of positive Chinese traditional culture in colleges and universities in the new media era by means of content transformation, as well as the online and offline combination from the aspects of communication mode, communication form and communication content.
Research on the Development of Voice Assistants in the Era of Artificial Intelligence
Authors: Huang Yuqi
Published: January 2023

Voice assistants have gradually occupied an important position in the products of many electronics companies. Artificial Intelligence voice assistants are able to interpret human speech and respond. Users can ask their assistant questions and manage other essential tasks such as email calendars through verbal commands. This paper analyzes the artificial intelligence voice assistant through the method of comparative analysis. The author studies the development situation of intelligent voice assistants, and compares the differences between Chinese and foreign voice assistants, and finally discusses the relationship between voice intelligent assistants and people’s lives. The author found that users in different countries have different functional preferences for using voice assistants, but they can help people’s work and life to a great extent. In other words, voice assistants play an important role in contemporary society. Therefore, people need to better understand the relationship between humans and machine