bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment preferences and current practices regarding open tibial shaft fractures
Authors: Qian Shengjun , Shen Yechao , Sun Lingling , Wang Zhan
Published: July 2024

Purpose

The purpose of this study was to reveal the treatment preferences and current practices regarding open tibial shaft fracture (OTSF).

Patients and methods

Online surveys of treatment preferences and current practice of OTSF were conducted by orthopedic trauma doctors from various medical institutions in Zhejiang Province. The survey contains three modules. The first module is the basic information of the participants, the second module is the treatment patterns for Gustilo-Anderson type I-II (GA I/II), and the third module is the treatment patterns for Gustilo-Anderson type IIIA (GA IIIA). Furthermore, each treatment pattern was divided into four aspects, including antibiotic prophylaxis, irrigation and debridement, fracture stabilization, and wound management.

Results

A total of 132 orthopedic trauma doctors from 41 hospitals in Zhejiang province, participated the online surveys. In GA I-IIIA OTSF, more than three-quarters of participants considered <3 h as the appropriate timing of antibiotic administration after trauma. In fact, only 41.67% of participants administered antibiotics within 3 h after trauma. 90.91 and 86.36% of participants thought debridement within 6 h was reasonable for GA I/II and GA IIIA OTSF, respectively. However, in reality only about half of patients received debridement within 6 h on average. The most common reason for delayed debridement was patients’ transport delay. 87.88 and 97.3% of participants preferred secondary internal fixation following external fixation for GA I/II and GA IIIA OTSF, respectively. Additionally, over half of participants preferred use of locking plate for treating GA I-IIIA OTSF. The most common reasons for choosing delayed internal fixation for GA I-IIIA OTSF were infection risk and damage control. 78.79 and 65.91% supported immediate internal fixation after removing the external fixation for GA I-IIIA OTSF, respectively. Regarding wound closure, 86.36 and 63.64% of participants reported primary closure for GA I/II and GA IIIA OTSF, respectively. Over three fourths of participants agreed that preoperative and postoperative multiple wound cultures should be performed to predict infection for GA I-IIIA OTSF.

Conclusion

The study first presents the current preference and practice regarding management of GA I-IIIA OTSF in Zhejiang. Majority of surgeons in our study preferred secondary internal fixation following external fixation for GA I-IIIA OTSF and over half of surgeons preferred use of locking plate for treating GA I-IIIA OTSF. This study may provide a reference for trauma orthopedic surgeons in the treatment of GA I-IIIA OTSF.
The honey bee “hive” microbiota: meta-analysis reveals a native and aerobic microbiota prevalent throughout the social resource niche
Authors: Anderson Kirk E. , Copeland Duan C.
Published: July 2024

The microbiome of the honey bee worker hindgut has been explored thoroughly with culturing and next-generation sequencing revealing both composition and function. However, less effort has been devoted to the aerobic social niches associated with the hive environment and colony process. We performed a meta-analysis of 3,800+ publicly available 16S rRNA gene sequence libraries examining the hypothesis of a native aerobic microbiota associated with social interaction and colony resources. We selected high-throughput studies to represent tissue-specific samples, including nine distinct aerobic niches throughout the colony and hive, defined by social nutrient processing. These included queen and worker gut tissues, foregut, midgut, ileum, rectum, mouthparts, worker social glands, developing larvae, and secreted and stored nutrition. We found that the aerobic mouthparts, foregut and midgut niches of queens and workers share a significant portion of their microbiome with that of larval rearing and nutrient secretion and storage, defining the microbiota of the social resource niche. Characterized by species dominance and rapid growth, the social resource microbiota functions primarily in disease prevention at both the individual and colony level and may also function in social communication and gut microbiome resilience. Defining the microbiota of social function contributes to a systems-level understanding of host–microbial interactions in the honey bee.
Engaging people with lived experience of dementia in research meetings and events: insights from multiple perspectives
Authors: Snowball Ellen , Aiken Christine , Norman Myrna , Hykaway Wayne , Dempster Zoe , Itzhak Inbal , McLellan Emily , McGilton Katherine S. , Bethell Jennifer
Published: July 2024

This perspective article describes the experiences of engaging people with lived experience of dementia in research meetings and events from the perspectives of people with lived experience, researchers, trainees, audience members and others. We outline examples of engagement from different events and describe a video project, initiated by people with lived experience, conveying diverse views about becoming integral collaborators in the Canadian Consortium on Neurodegeneration in Aging (CCNA) annual Partners Forum and Science Days. We also report evaluation data from audiences and present a series of tips and strategies for facilitating this engagement, including practical considerations for supporting people with lived experience.
Word-of-mouth in agent-based simulation model of reverse logistics
Authors: Labelle Alexandre , Frayret Jean-Marc
Published: July 2024

Agent-based modeling and simulation is a method well suited for studying individual behavior and interactions among members of a population connected by social networks. Although the development of such simulation models can be relatively complex, it is even more challenging to develop models that are empirically valid. In the case of reverse logistics, the sophisticated and difficult-to-predict behavior of consumers must be modeled. In this paper, an agent-based simulation model of consumer behavior and interactions was configured to conduct a case study of the voluntary deposit collection program for wine bottles in the Val-Saint-François region of Quebec. As this collection program was officially launched in 2019, two empirical samples were obtained to test the validity of the model and study how social interactions such as word of mouth contributes to the success of the collection program. The first sample represents the amount of glass collected during the last 26 weeks of 2019, while the second sample covers the first 13 weeks of 2020. Having observed an increase in collection rates between 2019 and 2020, word of mouth was introduced into the model to explain this phenomenon. Statistical tests show that the model is indeed valid with the inclusion of diffusion of awareness, as the simulation results are significantly consistent with the empirical data. The validation of the model demonstrates the viability of using multiple heterogeneous data-sources to configure a simulation model based on the Theory of Planned Behavior without using Structural Equation Modeling.
Tracing the journey of elements from fish feed to Nile tilapia faeces to black soldier fly larvae: a comparative approach
Authors: Yakti Wael , Shaw Christopher , Müller Marcus , Mewis Inga , Kloas Werner , Ulrichs Christian
Published: July 2024

Introduction

The circular bioeconomy concept revolves around biological production cycles that reintroduce products or waste from one production system to another, aiming to maximize resource utilization while minimising environmental impact. The purpose of this study was to evaluate and compare the element flow when integrating black soldier fly larvae (BSF) production with Nile tilapia production using varying experimental fish feed.

Methods

Tilapia (42.5  ± 11.2  g) were reared in recirculating aquaculture systems (RAS) at 25.5°C for 10  weeks and fed equal daily rations of four experimental diets containing fishmeal (FM), poultry blood meal (PBM), black soldier fly meal (BSF) and poultry by-product meal (PM) as the single main protein source, respectively. Faeces was collected daily from settling columns installed in the RAS and subsequently fed to BSF larvae.

Results and discussion

The fish exhibited the highest biomass gain when fed with FM (1,001  g) or PM (901  g). The growth was lowest for those fed with PBM (406  g). The fish fed with PBM also produced the highest amount of faeces (234 g). When the fish faeces were utilized as a substrate for rearing black soldier fly (BSF) larvae and although the biomass gain did not differ significantly, the feed conversion ratio (FCR) varied among larvae fed with different fish faeces, ranging from 8.36 to 25.04. Furthermore, the concentration of analysed elements (Al, B, Ca, Co, Cu, Fe, K, Mg, Mn, Mo, Na, P, S, and Zn) varied based on the type of fish faeces provided. Results illustrate that a modulation of BSFL elemental composition is possible through manipulating the feed provided to the fish and emphasize the importance of fish feed composition for fish performance as well as the compositional quality of the larvae reared on the resulting fish faeces. Hence, if BSF larvae production is to be harnessed for the recycling of aquaculture sludge, ingredient choice and subsequent elemental composition of fish feeds are pivotal to larvae composition.
Early clinical outcomes of bipolar hemiarthroplasty for femoral neck fractures in elderly patients using the OCM approach: a retrospective study
Authors: Zheng Hongming , Kong Danhui , He Shuangjun , Jiang Boyi , Zhu Dongbo , Wu Shuhua , Wang Yaowei , Zhou Lijian , Xia Yan
Published: July 2024

Objective

This study aims to assess the early clinical outcomes of bipolar hemiarthroplasty for treating femoral neck fractures in elderly patients aged 75 and above using the Orthopädische Chirurgie München (OCM) approach.

Methods

A retrospective analysis was conducted on a cohort of 95 elderly patients who underwent bipolar hemiarthroplasty for Garden Type III and IV femoral neck fractures between January 2020 and December 2022. The participants were categorized into two groups according to the surgical approach used: the OCM approach and the posterior-lateral approach (PLA). The average follow-up duration was 11.20 ± 2.80 months for the OCM group and 11.12 ± 2.95 months for the PLA group, with both groups ranging from 6 to 18 months. Clinical outcomes assessed included surgical duration, incision length, postoperative hospital stay, time to ambulation, hemoglobin levels, serum creatine kinase (CK) levels, C-reactive protein (CRP) levels, pain (assessed using the Visual Analogue Scale, VAS), and functional recovery (evaluated through Harris hip scores). Additionally, complications such as intraoperative and postoperative fractures, deep vein thrombosis, wound infection, nerve injury, postoperative dislocation, leg length discrepancy, and Trendelenburg gait were monitored.

Results

There was no significant difference in the surgical duration between the OCM and PLA groups. However, the OCM group exhibited shorter incision lengths, reduced postoperative hospital stays, and earlier ambulation times compared to the PLA group. Significantly lower intraoperative blood loss, smaller decreases in hemoglobin levels on postoperative days 1 and 3, lesser hidden blood loss, and decreased levels of CK and CRP were observed in the OCM group. Pain levels, measured by VAS scores, were lower, and Harris hip scores, indicating functional recovery, were higher at 2 and 6 weeks postoperatively in the OCM group than in the PLA group. The incidence of complications, such as periprosthetic fractures, intramuscular venous thrombosis, hip dislocations, Trendelenburg gait, and leg length discrepancies, showed no significant differences between the groups.

Conclusion

The OCM approach for bipolar hemiarthroplasty in patients aged 75 and above with femoral neck fractures offers significant early clinical benefits over the traditional PLA, including faster recovery, reduced postoperative pain, and enhanced early functional recovery.
Effects of prebiotic (lactoferrin) and diclazuril on broiler chickens experimentally infected with Eimeria tenella
Authors: Abd El Monsef Asmaa G. , El Zohairy Nermin F. , Hassan Marwa F. , Salem Sanaa M. , Gouda Asmaa Aboelabbas , Mansour Mogda K. , Alkhaldi Abdulsalam A. M. , Alzaylaee Hind , Elmahallawy Ehab Kotb
Published: July 2024

Introduction

Avian coccidiosis presents a significant challenge to the poultry industry in Egypt, highlighting the urgent need for validating new drug targets offering promising prospects for the development of advanced anticoccidials. Although numerous reports highlight the activity of lactoferrin (LF) against various microorganisms, its potential against Eimeria has not been explored. The present study evaluated the potential anticoccidial effect of LF and diclazuril in broiler chickens experimentally infected with Eimeria tenella.

Methods

A total of 100 one-day-old broiler chicks were divided into five equal groups (20 each) as follows: Group 1 (G1) served as the normal healthy control group, Group 2 (G2) consisted of chickens infected with 1 × 105 sporulated E. tenella oocysts at 14 days of age, Group 3 (G3) comprised infected chickens treated with diclazuril (0.5 mL/L in drinking water) for 3 days successively, Group 4 (G4) included infected chickens treated with LF (at a dose of 250 mg/kg of diet) from one day of age until the end of the study, and Group 5 (G5) comprised infected chickens treated with both LF and diclazuril.

Results

The positive control group (G2) experienced significant reductions in body weight (BW), BW gain, serum glucose, lipase, amylase, total antioxidant capacity, several hematological indices, and total proteins, along with alterations in various antioxidant enzymes. Conversely, serum levels of aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatases (ALP), urea, creatinine, nitric oxide, mean corpuscular volume (MCV), White blood cells (WBCs), heterophils, alpha 2, beta 1, and liver contents of malondialdehyde were elevated in this group. Moreover, higher oocyst counts and lesion scores, along with histopathological alterations, were observed in G2. Remarkably, treatment with diclazuril and/or LF demonstrated potent antioxidant and anticoccidial effects, resulting in reduced shedding of oocysts, lesion scores, and lymphocytic infiltrates in the cecum. Additionally, these treatments improved the antioxidant and immune systems in chickens and restored all histopathological changes reported in the infected non-treated group (G2).

Conclusion

This study offers novel perspectives on the potential anticoccidial effects of the combination of LF and diclazuril in broiler chickens infected with E. tenella, highlighting the potential synergistic actions of LF in treating poultry coccidiosis.
Use of wound infusion catheters for delivery of local anesthetic following standing partial ostectomy of thoracolumbar vertebral spinous processes in horses is not associated with increased surgical site infections
Authors: Wickstead Francesca A. , Milner Peter I. , Bardell David A.
Published: July 2024

Background

Wound infusion catheters (WICs) have been used in humans and some veterinary species for post-operative local anesthetic administration following a variety of surgical procedures, aiming to reduce post-operative analgesia requirements and improve patient comfort. Benefit in reduction in pain, post-operative analgesia requirements and length of hospital stay are well documented in humans, but use of WICs may not have been widely adopted in veterinary species due to the concern of increased complications, such as dehiscence or surgical site infection (SSI), creating a barrier to their use. This study aimed to evaluate the use of WICs in horses undergoing standing partial ostectomy surgeries, document complications and investigate if the incidence of SSI was equivalent between those horses that did and did not have a WIC.

Methods

Clinical records were searched between January 2010–December 2023 for horses undergoing standing partial ostectomy surgery of thoracolumbar vertebral spinous processes at one institution. Population variables (age, breed, bodyweight), placement of a WIC or not, post-operative complications, analgesia protocols and surgical time were recorded. Horses received up to 0.1 mg kg−1 bupivacaine (0.5 mg mL−1) every 6–8 h via the WIC where one was placed. To compare SSI complication incidence between using or not using a WIC, a proportional independent equivalence test was used.

Results

There were 64 horses included in the final analysis with a WIC placed in 29/64 horses (45.3%) and 35/64 (54.7%) having no WIC placed at surgery. Incidence of short-term SSI was 11.4% (no WIC used) and 13.8% (WIC used), respectively. The difference in proportion of SSI between the presence or absence of a WIC was not significant [−0.024 (90% CI −0.181; 0.133); p = 0.94].

Conclusion

The incidence of SSIs was equivalent between groups whether a WIC was used or not. WICs should be considered as part of a multi-modal analgesic approach in the post-operative period. Further research into local anesthetic dosing and its impact on rescue analgesia requirements and pain-scores is warranted.
Inter-Satellite Link Prediction with Supervised Learning: An Application in Polar Orbits
Authors: Ferrer Estel , Ruiz-De-Azua Joan A. , Betorz Francesc , Escrig Josep
Published: July 2024

Distributed space systems are increasingly valued in the space industry, as they enhance mission performance through collaborative efforts and resource sharing among multiple heterogeneous satellites. Additionally, enabling autonomous and real-time satellite-to-satellite communications through Inter-Satellite Links (ISLs) can further increase the overall performance by allowing cooperation without relying on ground links and extensive coordination efforts among diverse stakeholders. Given the constrained resources available onboard satellites, a crucial element of achieving cost-effective and autonomous cooperation involves minimizing energy wastage resulting from unsuccessful or unnecessary communication. To address this challenge, satellites must anticipate their ISL opportunities or encounters with minimal resource utilization. Building upon prior publications, this work presents further insights into the use of supervised learning to enable satellites to forecast their encounters without relying on orbit propagation. In particular, a more realistic definition of satellite encounters, along with a versatile solution applicable to all polar low-Earth orbit satellites is implemented. Results show that the trained model can anticipate encounters for realistic and unseen data from an available data source with a balance accuracy of around 90% and six times faster when compared with the well-known Simplified General Perturbation 4 orbital model.
Numerical Investigation of Flame-Acoustic Interaction at Resonant and Non-Resonant Conditions in a Model Combustion Chamber
Authors: Horchler Tim , Fechter Stefan , Hardi Justin
Published: July 2024

Despite considerable research effort in the past 60 years, the occurrence of combustion instabilities in rocket engines is still not fully understood. While the physical mechanisms involved have been studied separately and are well understood in a controlled environment, the exact interaction of fluid dynamics, thermodynamics, chemical reactions, heat-release and acoustics, ultimately leading to instabilities, is not yet known. This paper focuses on the investigation of flame-acoustic interaction in a model combustion chamber using detached-eddy simulation (DES) methods. We present simulation results for a new load point of combustion chamber H from DLR Lampoldshausen and explore the flame response to resonant and non-resonant external excitation. In the first part of the paper, we use time-averaged results from a steady-state flow field without siren excitation to calculate the combustion chamber Helmholtz eigenmodes and compare them to the experimental results. The second part of the paper presents simulation results at a non-resonant excitation frequency. These results agree very well with the experimental results at the same condition, although the numerical simulation systematically overestimates the oscillation amplitudes. In the third part, we show that a simulation with resonant siren excitation can correctly reproduce the shift in eigenmode frequencies that is also seen in the experiments. Additionally, for this new load point, we confirm previous numerical results showing a strong influence of transversal excitation on the shape of the dense LOx cores. This work also proposes a bombing method for determining the resonant eigenmode frequencies based on an unexcited steady-state DES by simulating the decay of a strong artificial pressure pulse inside the combustion chamber.
Loop-Ultrasound-Assisted Extraction: An Efficient Approach for the Recovery of Bioactive Compounds from Oak Bark
Authors: Quaratesi Ilaria , Calinescu Ioan , Lavric Vasile , Ferrara Vittoria , Badea Elena , Chipurici Petre , Dumbravă Elisa-Gabriela , Constantinescu Rodica-Roxana , Ignat Nicoleta Daniela , Popa Ioana
Published: July 2024

Oak bark is a by-product known for its richness in polyphenols, with tanning substances being particularly interesting for their application in different fields. Vegetable tannins are mostly utilized in the leather sector, but are also widely used as adhesives, in cement plasticizers and for medical and agrochemical applications owing to their natural antimicrobial activity. This study aimed to develop a green and efficient pilot-scale technique for extracting polyphenols from oak bark by ultrasound-assisted extraction (UAE) using a modified Dual-Frequency Reactor (DFR). Different parameters, such as extraction time, temperature, and solvent type (water, sodium hydroxide or sodium sulfite and bisulfite solutions) were investigated for their influence on the total phenolic content (TPC) and the quantity of dry extract. Control experiments by conventional methods were also performed. UAE at 50 °C yielded the highest TPC and dry extract (confirmed by ANOVA analysis, p < 0.05) in just 10 min, suggesting that UAE can be considered an energy- and cost-effective alternative to conventional techniques. The most suitable solvent was found to be a 0.5% sodium hydroxide solution. The molecular profile of the extracts was assessed by FTIR-ATR spectroscopy, revealing typical signals of tannins in all extracts. Furthermore, antimicrobial activity tests demonstrated the complete absence of Gram-positive and Gram-negative bacteria in the extracts, ensuring the suitability of the product for different kinds of application.
Responses of Rare and Abundant Bacterial Communities to Synergistic Phosphate Fertilization and Trichoderma Inoculation Meant to Improve Alfalfa Yields
Authors: Zhou Zhibo , Ding Yongyong , Liu Weijie , Jia Zhenyu , Zhang Fengge
Published: July 2024

In the field of agro-grassland, the synergism of phosphate (P) fertilization and Trichoderma inoculation in alfalfa production and the underlying mechanism of rare and abundant microbes that regulate rhizosphere soil processes in various environments are key but rarely studied topics. Here, we conducted field research through the inoculation/noninoculation of Trichoderma and five levels of phosphorus fertilizer to explore the biological relationships of rhizosphere soil properties, rare and abundant taxa, and alfalfa yields. Our results demonstrate that using Trichoderma inoculation and 15 g/m2 of phosphorus fertilizer exhibited optimal alfalfa yield compared with other treatments. alfalfa yields significantly (R2 = 0.33; p < 0.001) increased along with soil fertility. Both rare (RANOSIM = 0.900; p = 0.001) and abundant (RANOSIM = 0.769; p = 0.001) bacterial communities were significantly different under Trichoderma inoculation and P fertilization in a nonmetric multidimensional scaling (NMDS) analysis. Furthermore, different ecological processes dominated the rare and abundant bacterial community assembly. PLS-PM analysis showed that Trichoderma inoculation positively regulated the abundant bacteria community and P fertilization regimes manipulated the rare bacteria community, synergistically contributing to alfalfa yields. Overall, this article believes that inoculation with Trichoderma and appropriate application of phosphorus fertilizer can significantly increase alfalfa yield and affect soil enzyme activity, and the rhizosphere soil abundant and rare bacterial community characteristics have different responses to the synergistic effect of Trichoderma and phosphorus fertilizer. Our research emphasizes the fundamental role of abundant and rare microbes in maintaining crop production using Trichoderma inoculation and P fertilization. Therefore, distinguishing rare and abundant species is beneficial to comprehensively understanding microbial-driven processes and providing theoretical support for maintaining ecosystem productivity.
Empirical Modelling of Power Requirements in Olive Pruning Residue Shredding: Effects of Varying Moisture Content and Rotary Speeds
Authors: Yiğit Mete , Çanakcı Murad , Karayel Davut
Published: July 2024

Pruning residues, which occur every year in orchards and have many different utilization potentials, are an important issue for fruit producers. The shredding process is indispensable and critical for the utilization of these residues. The performance of the shredding process is affected by the operating parameters of the shredding machine as well as the moisture content of the residues to be shredded. In this study, olive pruning residues with three different moisture contents were shredded at three different rotor speeds in the developed shredding system. We determined how the power requirement of the shredder changed under different conditions, and empirical models were developed. The experiments showed that the average power requirement of the shredder ranged from 7.32 to 10.81 kW, and it was found that residues with low moisture content decreased the power values, while higher rotor speeds increased the power requirement. The developed final model has a mean absolute error (MAE) of 0.376, a root mean square error (RMSE) of 0.441, and a correlation coefficient (R2) of 0.859. The model serves as a reliable tool for estimating power requirements in the shredding of olive pruning residues, enabling the selection of the optimal rotor speed based on moisture content.
Genome-Wide Analysis of Soybean Mosaic Virus Reveals Diverse Mechanisms in Parasite-Derived Resistance
Authors: Yang Na , Qiu Yanglin , Shen Yixin , Xu Kai , Yin Jinlong
Published: July 2024

Plant viruses cause severe losses in agricultural production. Parasite-derived resistance (PDR) offers a promising avenue for developing disease-resistant varieties independent of resistance genes. However, for potyviruses with great agricultural importance, such as soybean mosaic virus (SMV), systematic research on viral genes that can be used for PDR has not been conducted. In this study, we transiently expressed the untranslated region (UTR) or each protein-coding cistron of SMV in Nicotiana benthamiana to evaluate their potential role in conferring PDR. A viral suppressor of RNA silencing (VSR) was also applied to investigate the possible mechanisms of the PDR. The results showed that the transient overexpression of UTR and each cistron of SMV could inhibit SMV infection. The expression of VSR in N. benthamiana leaves could compromise UTR and most of the SMV cistron-mediated inhibition of SMV infection, indicating the involvement of RNA silencing in PDR. In comparison, the expression of VSR could not compromise the PDR conferred by coat protein (CP), P3N-PIPO, cylindrical inclusion (CI), and NIa-Pro, suggesting that these viral cistrons may play roles in PDR at the protein level. These results reveal diverse mechanisms in PDR conferred by different viral cistrons and provide potential gene candidates that can be used for transgenic approaches against SMV.
Predictors for Green Energy vs. Fossil Fuels: The Case of Industrial Waste and Biogases in European Union Context
Authors: Popescu Catalin , Gabor Manuela Rozalia , Stancu Adrian
Published: July 2024

In the context of sustainability, the integration of renewable energy into industrial processes not only minimizes dependence on fossil fuels but also contributes to the efficient management of industrial waste. By transforming organic waste, including agri-food and urban waste, into biogas, green energy can be generated, thus reducing the impact on the environment and closing the loop of material used in the economic circuit. Thus, a sustainable system can be promoted, where resources are continuously reused and exploited. Statistical methods and a decision tree with the Classification and Regression Trees (CRT) algorithm were employed to analyze data. The paper focuses on the importance of industrial waste and biogas for the generation, transformation, and consumption of energy in the EU (European Union)-27 countries. To provide a thorough analysis, we have divided these countries based on real gross domestic product (GDP) per capita, grouping them above/below the annual average for the period 2012–2021/2022. Descriptive statistics revealed observable differences between the two groups, but the paper aimed to provide evidence regarding the existence of these differences as statistically significant. Using the Kolmogorov–Smirnov test, the non-normal distribution of the data was confirmed, requiring non-parametric inferential methods. The Mann–Whitney U test revealed statistically significant differences between the two groups for all the studied variables. This comprehensive approach highlights the distinct energy-related characteristics influenced by economic development in the EU-27.
Traits of Mortadella from Meat of Different Commercial Categories of Indigenous Dairy Cattle
Authors: Giosuè Cristina , Maniaci Giuseppe , Gannuscio Riccardo , Ponte Marialetizia , Pipi Marianna , Di Grigoli Antonino , Bonanno Adriana , Alabiso Marco
Published: July 2024

The rising interest in healthier meat options prompted the exploration of alternatives to traditional pork-based products, incorporating meat from different livestock species, feeding regimens, and functional ingredients. This study investigates the production of healthier meat products by examining the physicochemical traits, fatty acid profile, and sensory properties of mortadella made with Cinisara meat of four young bulls and four adult cows, and four females of the Nebrodi Black Pig. All the animals were fed principally on natural resources. Nutritional analysis revealed different levels of moisture, protein, fat, and ash in raw materials, with pistachios contributing to a healthy fatty acid profile rich in monounsaturated and polyunsaturated fatty acids. Formulations using cow meat exhibited higher fat content and caloric value, resulting in sensory attributes such as more intense color, improved fat cube adhesion, and pronounced odors compared to young bull and control mortadella. Fatty acid analysis demonstrated distinctive profiles influenced by the meat type used and, as expected, bovine products showed higher contents of rumenic and other conjugated linoleic acids. Pork mortadella displayed greater ω6 and ω3 values, with a healthier ω6/ω3 ratio comparable to those found in cow products. Young bull mortadella showed the worse atherogenic and thrombogenic indices. The findings underscore the impact of raw materials on the nutritional and sensory attributes of mortadella, emphasizing the necessity for interventions to enhance fatty acid composition in processed meat products.
Effects of Steviol Glycosides on Growth Performance, Ruminal Fermentation and Microbial Diversity of Hu Sheep
Authors: Zhang Jianeng , Li Xiongxiong , Sha Yuzhu , Wang Zhengwen , Qi Shuai , Zhang Xia , Zhao Shengguo , Jiao Ting
Published: July 2024

The experiment was conducted to investigate the potential effects of steviol glycosides on growth performance, rumen fermentation processes, and microbial diversity in Hu sheep. A single-factor design was used for the trial. Twenty healthy weaned Hu lambs, possessing comparable body weights averaging 18.31 ± 1.24 kg, were randomly allocated into two distinct groups: the control group (CON) and the experimental group (STE), with each comprising 10 lambs. The CON was fed the basal diet, and the STE was supplemented with 0.07% steviol glycosides based on the basal diet. During the experimental period, variations in body weight and feed intake were closely monitored and recorded. After feeding for 90 d, blood was collected to determine blood biochemical indices, and rumen fluid samples were gathered for an in-depth analysis of rumen fermentation parameters and microbial diversity. The outcomes revealed no statistically significant differences in growth performance or serum biochemical indices between the two groups (p > 0.05). Rumen pH in STE and CON was within the normal range. The rumen ammonia nitrogen (NH3-N) and acetic acid (AA) content of STE decreased significantly compared with CON (p < 0.05). No significant variations were observed in the levels of other volatile fatty acids (VFAs) between the two groups (p > 0.05). The rumen microbial OTUs count, as well as the Shannon, Simpson, Chao1, and Ace indices, were notably lower in the STE group compared to the CON group (p < 0.05). Additionally, at the phylum level, the relative abundance of Firmicutes, Bacteroidetes, and Proteobacteria collectively accounted for over 97% of the total phylum composition. In comparison to the CON group, the STE group exhibited an increase in the relative abundance of Proteobacteria (p < 0.05), accompanied by a significant reduction in the relative abundance of Patescibacteria and Desulfobacteria (p < 0.05). At the genus level, there was a notable increase in the relative abundance of Prevotella_7 and Succinivibrionaceae_UCG_001 in the STE group, whereas the relative abundance of Rikenellaceae_RC9_gut_group significantly decreased (p < 0.05). According to the correlation analysis between rumen microflora and VFAs, the relative abundance of Succinivibrionaceae_UCG_001 displayed a significant negative correlation with AA (p < 0.05), whereas Lactobacillus exhibited a notable positive correlation with isobutyric acid (IBA) (p < 0.05). In summary, steviol glycosides had no significant effect on the production performance and blood biochemical indexes of Hu sheep. Steviol glycosides can improve rumen fermentation parameters and rumen microflora structure of Hu sheep and have a certain effect on rumen microbial diversity and composition.
Lithium Battery SoC Estimation Based on Improved Iterated Extended Kalman Filter
Authors: Wang Xuetao , Gao Yijun , Lu Dawei , Li Yanbo , Du Kai , Liu Weiyu
Published: July 2024

With the application of lithium batteries more and more widely, in order to accurately estimate the state of charge (SoC) of the battery, this paper uses the iterated extended Kalman filter (IEKF) algorithm to estimate the SoC. The Levenberg–Marquardt (LM) method is used to optimize the error covariance matrix of IKEF. Based on the hybrid pulse power characteristics experiment, a second-order Thevenin model with variable parameters is established on the MATLAB platform. The experimental results show that the proposed model is effective under the constant current discharge condition, the Federal Urban Driving Schedule (FUDS) condition, and the Beijing dynamic stress test (BJDST) condition. The results show that the simulation error of the improved LM-IEKF algorithm is less than 2% under different working conditions, which is lower than that of the IKEF algorithm. The improved algorithm has a fast convergence speed to the true value, and it has a good estimation accuracy in the case of large changes in external input current. Additionally, the fluctuation of error is relatively stable, which proves the reliability of the algorithm.
Dynamic Characteristics of λ-DNA Molecules Translocating through Tapered Microfluidic Channel System Driven by Electric Field Force
Authors: Niu Yong , Zhu Jie , Wang Jianfei , Li Yanjie , Liu Yanfei , Li Zhiwei , Dang Yang , Sun Dan , Wang Kaige
Published: July 2024

The dynamic characteristics of single DNA molecules translocating within micro/nano-channels are fundamental for a wide range of applications such as stretching, separating, mapping, and even sequencing of DNA molecules. In this study, a type of tapered microchannel chip with uniform height for all configurations was fabricated, with the major tapered structure having a length of 13 μm and a width that tapers from 5 μm to 20 μm. The dynamic characteristics such as the trajectories and velocities of λ-DNA molecules translocating from different positions driven by an external DC electric field force were systematically investigated by single-molecule fluorescence imaging technology. Some dynamic characteristics of DNA molecules translocation were found. Considering simply the effects of electrophoretic force and electro-osmotic force on the DNA molecules, the dynamic characteristics of DNA molecules are well understood. For example, the velocity of the DNA molecule is inversely proportional to the diameter of the tapered channel and the turning phenomena of the trajectory of the DNA molecules translocating through microchannels. This study is helpful and proposes new ideas for the design and development of microfluidic chips for the quantitative manipulation of DNA molecules.
Radiotherapy-Related Clinical and Functional Sequelae in Lung Cancer Survivors
Authors: Heredia-Ciuró Alejandro , Blasco-Valls Paula , Calvache-Mateo Andrés , Valenza-Peña Geraldine , López-López Laura , Ortiz-Rubio Araceli , Valenza Marie Carmen
Published: July 2024

It is known that lung cancer survivors experience sequelae due to the evolution of the disease and oncological treatment. However, there is no consensus about their sequelae after radiotherapy treatment. The aim of this study was to analyze the clinical–functional profile of lung cancer survivors who receive radiotherapy treatment. This prospective observational study evaluated lung cancer patients who were candidates for radiotherapy treatment in the oncological radiotherapy unit of San Cecilio University Hospital in Granada. Symptoms (i.e., fatigue, cough, and dyspnea), functionality (i.e., physical activity levels and muscle mass), and quality of life were evaluated pre- and post-radiotherapy treatment. Sixty-five participants were included, and sixty-one completed both evaluations. Patients showed a significant increase in symptoms (p < 0.05) and a decline in functionality (p < 0.001) after completing radiotherapy. Quality of life was significantly lower after completing radiotherapy treatment (p < 0.05). Lung cancer survivors showed an increase in symptoms, a decline in physical activity levels, and a decline in perceived quality of life after receiving radiotherapy treatment. These results provide more accurate knowledge about the functional impairment experienced by lung cancer patients and can facilitate the future management of impairment in lung cancer patients, thus improving their quality of life.
Recent Studies on the Healing Properties of Eicosapentaenoic Acid
Authors: Dospra Maria , Pavlou Panagoula , Papageorgiou Spyridon , Varvaresou Athanasia
Published: July 2024

Patients with diabetes, the elderly, and those who have certain inherited conditions are particularly vulnerable to atypical wound healing with lingering repercussions. Remarkably, the current remedies are insufficient since, despite the plethora of wound healing options, only limited efficacy is observed. This review is a bibliographic survey on eicosapentaenoic acid and its healing effects. It has been investigated in terms of its source of origin, structure, physico-chemical properties, and studies where healing action is demonstrated. Fatty acids, found in all layers of the skin, modify cell function and the synthesis of eicosanoids, reactive oxygen species, and cytokines, which affects skin structure and immunological condition. As a result, fatty acids influence both the inflammatory response and the wound-healing process. EPA is one of the dietary lipids that has a variety of health advantages. It functions in anti-inflammatory processes and the firmness of cell membranes and is integrated into numerous bodily parts. EPA has a crucial role in healthy fetal development and aging. It is a precursor to numerous metabolites that are powerful lipid mediators and are regarded by many researchers as being helpful in the treatment or prevention of several disorders. EPA supplement is used after surgery to lessen infections, accelerate wound healing, and speed up recovery, although, according to other researchers, the oral administration of omega-3 fatty acids, particularly the DHA and EPA combination, significantly slows down the healing of wounds and disrupts the structure of collagen through several mechanisms. The controversy in the reported literature is discussed and new technologies useful for the improvement of the wound healing process are also reported.
Experimental Bond Characterisation and Inverse Numerical Stress-Slip Law Identification for Adhesive Anchor Applications
Authors: Marcon Marco , Vorel Jan , Ninčević Krešimir , Wan-Wendner Roman
Published: July 2024

In order to determine the adhesive anchors’ capacity under tensile loading, two test methods (confined and unconfined) are suggested in the guideline. Improvements for one of the two configurations are proposed and tested in this paper. The alternative setup currently being evaluated is a modified version of the one proposed by the RILEM technical recommendation, which is used to determine the bond properties of reinforced concrete elements. Finding a better and more consistent definition of the bonded length, removing the concrete surface contribution from the determined bond resistance, and testing the stability improvements were considered during the proposed setup development. In order to describe the adhesive anchor system’s behaviour, a stress-slip relation (law), which relates the local axial bond stress to the local axial slippage, can be used. Analytical and numerical procedures have been proposed in the literature in order to determine such a law. Here, an inverse calibration method based on Sequentially Linear Analysis (SLA) is applied in order to identify the stress-slip law of pull-out tests.
Development of Thrust, Torque, and Power Estimation Model, and Prediction Performance of Earth Pressure Balance Tunnel Boring Machine in Mixed-Face Strata
Authors: Zhai Shufang , Song Yingjie , Tian Hao
Published: July 2024

In this paper, a theoretical estimation model of TBM thrust, torque, and power in the rock–soil interface (RSI) of mixed ground is developed, including a new force model for the drag cutter that accounts for chamber pressure and soil friction. A distribution model of the disc cutters and drag cutters on the cutterhead adaptable to different excavation surfaces is built in order to visualize the cutting process as the TBM cutterhead rotates, and a program is created and that runs smoothly using the Python version 3.8, which can recognize the numbers and calculate the forces of the disc cutters and drag cutters in the soft and hard strata, respectively. Then, combining with friction forces and chamber pressure calculated by the program, the variation in torque, thrust, and power are produced. Subsequently, a new index (MPPI), which considers both the thrust of the TBM and cutterhead torque, for forecasting TBM tunneling performance in composite strata is presented. The reliability of the index as well as the estimation model are validated using data from an actual project to offer recommendations for future tunneling projects.
STM-Suite, an Online Platform for the Assessment of Memory Functions Discriminates among Subgroups of Children with Different Types of Specific Learning Disorders
Authors: Giorgetti Marisa , Bombacigno Roberto , Toraldo Alessio , Lorusso Maria Luisa
Published: July 2024

A deficit in short-term memory (STM) functions characterizes many neurodevelopmental disorders, in particular, specific learning disorders. Hence, there is a need to develop a web-based platform capable of testing specific variables and administration conditions in a controlled manner. The platform herein presented allows for the assessment of short-term memory (STM) items and order components in a series of different conditions. Stimulus types, presentation, and response modalities were appropriately selected to assess the impact of those variables on memory performances. The usefulness of such a systematic, fine-grained analysis of STM functions was tested by applying the complete assessment in a group of 100 school-age children (47 Typically Developing children and 53 children with learning disorders) and evaluating the capacity of the software to highlight different specific memory processes activated during reading, writing, and calculation. A cluster analysis was applied to the learning performances of the whole group, and a four-cluster solution representing the best division into subgroups of learning disorders (affecting reading, writing, and mathematical skills, variously combined) also showed clear-cut differences in the children’s STM profiles. This confirms the potential and the usefulness of the tool for the characterization of STM in school-age children.
Digital Orofacial Identification Technologies in Real-World Scenarios
Authors: Corte-Real Ana , Ribeiro Rita , Almiro Pedro Armelim , Nunes Tiago
Published: July 2024

Three-dimensional technology using personal data records has been explored for human identification. The present study aimed to explore two methodologies, photography and orofacial scanning, for assessing orofacial records in forensic scenarios, highlighting their impact on human identification. A pilot and quasi-experimental study was performed using Canon 5D-Full Frame equipment (Tokyo, Japan) and an i700 scanner (Medit, Lusobionic, Portugal) (Seoul, Republic of Korea) with Medit Scan for Clinics (MSC) and Smile Design software (V3.3.2). The sample included living patients (n = 10) and individuals in forensic cases (n = 10). The study was divided into two complementary phases: (i) data collection using 2D and 3D technologies and (ii) visual comparison by superimposition procedures, 3D dental images with 3D facial records (3D–3D), and 2D photography with screen printing of 3D facial records (2D-3S). Statistical analyses were performed using descriptive procedures (Likert scale) and the Mann–Whitney U test. The Mann–Whitney U test comparing the data (n = 220 records) from living individuals and those in forensic cases identified statistically significant differences in the performance of the photographic methods for evaluating intraoral mineralisation (p = 0.004), intraoral soft tissues (p = 0.016), intraoral distortion (p = 0.005) and the scan methods for intraoral extra devices (p = 0.003) and extraoral soft tissues (p = 0.005). A visual comparison (n = 40) allowed 3D–3D superimposition. Additionally, 2D-3S superimposition qualitatively identified the middle third of the face as the corporal area within the anatomical features required for successful surgery. In conclusion, the present study presented evidence-based data suggesting that the IO scan method, as an emergent technology, should be explored as a valuable tool in forensic facial identification in real-world scenarios.