bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitochondria-Related TFAM and POLG Gene Variants and Associations with Tumor Characteristics and Patient Survival in Head and Neck Cancer
Authors: Golubickaite Ieva , Ugenskiene Rasa , Bartnykaite Agne , Poskiene Lina , Vegiene Aurelija , Padervinskis Evaldas , Rudzianskas Viktoras , Juozaityte Elona
Published: February 2023

In 2020, 878,348 newly reported cases and 444,347 deaths related to head and neck cancer were reported. These numbers suggest that there is still a need for molecular biomarkers for the diagnosis and prognosis of the disease. In this study, we aimed to analyze mitochondria-related mitochondrial transcription factor A (TFAM) and DNA polymerase γ (POLG) single-nucleotide polymorphisms (SNPs) in the head and neck cancer patient group and evaluate associations between SNPs, disease characteristics, and patient outcomes. Genotyping was performed using TaqMan probes with Real-Time polymerase chain reaction. We found associations between TFAM gene SNPs rs11006129 and rs3900887 and patient survival status. We found that patients with the TFAM rs11006129 CC genotype and non-carriers of the T allele had longer survival times than those with the CT genotype or T-allele carriers. Additionally, patients with the TFAM rs3900887 A allele tended to have shorter survival times than non-carriers of the A allele. Our findings suggest that variants in the TFAM gene may play an important role in head and neck cancer patient survival and could be considered and further evaluated as prognostic biomarkers. However, due to the limited sample size (n = 115), further studies in larger and more diverse cohorts are needed to confirm these findings.
MCD Inhibits Lipid Deposition in Goat Intramuscular Preadipocytes
Authors: Yang Changheng , Li Qi , Lin Yaqiu , Wang Yong , Shi Hengbo , Huang Lian , Zhao Wangsheng , Xiang Hua , Zhu Jiangjiang
Published: February 2023

Malonyl-CoA decarboxylase (MCD) is a major regulator of fatty acid oxidation catalyzing the decarboxylation of malonyl coenzyme A (malonyl-CoA). Although its involvement in human diseases has been well studied, its role in intramuscular fat (IMF) deposition remains unknown. In this present study, 1726 bp of MCD cDNA was cloned (OM937122) from goat liver, including 5′UTR of 27 bp, 3′UTR of 199 bp, and CDS of 1500 bp, encoding 499 amino acids. In this present study, although the overexpression of MCD increased the mRNA expression of FASN and DGAT2, the expression of ATGL and ACOX1 was also activated significantly and resulted in a decrease in cellular lipid deposition in goat intramuscular preadipocytes. Meanwhile, the silencing of MCD increased the cellular lipid deposition and was accompanied by the expression activation of DGAT2 and the expression suppression of ATGL and HSL, despite the expression suppression of genes related to fatty acid synthesis, including ACC and FASN. However, the expression of DGAT1 was not affected significantly (p > 0.05) by the expression alteration of MCD in this present study. Furthermore, 2025 bp of MCD promoter was obtained and predicted to be regulated by C/EBPα, SP1, SREBP1, and PPARG. In summary, although different pathways may respond to the expression alteration of MCD, the expression of MCD was negatively correlated with the cellular lipid deposition in goat intramuscular preadipocytes. These data may be beneficial for enhancing our understanding of the regulation of IMF deposition in goats.
Inflammatory Biomarkers Differ among Hospitalized Veterans Infected with Alpha, Delta, and Omicron SARS-CoV-2 Variants
Authors: Park Catherine , Tavakoli-Tabasi Shahriar , Sharafkhaneh Amir , Seligman Benjamin J. , Hicken Bret , Amos Christopher I. , Chou Andrew , Razjouyan Javad
Published: February 2023

Mortality due to COVID-19 has been correlated with laboratory markers of inflammation, such as C-reactive protein (CRP). The lower mortality during Omicron variant infections could be explained by variant-specific immune responses or host factors, such as vaccination status. We hypothesized that infections due to Omicron variant cause less inflammation compared to Alpha and Delta, correlating with lower mortality. This was a retrospective cohort study of veterans hospitalized for COVID-19 at the Veterans Health Administration. We compared inflammatory markers among patients hospitalized during Omicron infection with those of Alpha and Delta. We reported the adjusted odds ratio (aOR) of the first laboratory results during hospitalization and in-hospital mortality, stratified by vaccination status. Of 2,075,564 Veterans tested for COVID-19, 29,075 Veterans met the criteria: Alpha (45.1%), Delta (23.9%), Omicron (31.0%). Odds of abnormal CRP in Delta (aOR = 1.85, 95% CI:1.64–2.09) and Alpha (aOR = 1.94, 95% CI:1.75–2.15) were significantly higher compared to Omicron. The same trend was observed for Ferritin, Alanine aminotransferase, Aspartate aminotransferase, Lactate dehydrogenase, and Albumin. The mortality in Delta (aOR = 1.92, 95% CI:1.73–2.12) and Alpha (aOR = 1.68, 95% CI:1.47–1.91) were higher than Omicron. The results remained significant after stratifying the outcomes based on vaccination status. Veterans infected with Omicron showed milder inflammatory responses and lower mortality than other variants.
Time to Lift up COVID-19 Restrictions? Public Support towards Living with the Virus Policy and Associated Factors among Hong Kong General Public
Authors: Mo Phoenix K. H. , Yu Yanqiu , Lau Mason M. C. , Ling Rachel H. Y. , Lau Joseph T. F.
Published: February 2023

The fifth wave of the COVID-19 pandemic has caused an unprecedented toll on Hong Kong. As more countries are starting to lift COVID-19 restrictions, it would be important to understand the public attitudes towards lifting COVID-19 restrictions and to identify its associated factors. The present study examined the level of support towards the living with the virus (LWV) policy for COVID-19 among the public in Hong Kong and to identify the associations between resilient coping, self-efficacy and emotional distress with support towards the LWV policy. A random population-based telephone survey was conducted among 500 Hong Kong Chinese adults from 7 March to 19 April 2022, i.e., during the fifth wave of COVID-19 outbreak. Of the respondents, 39.6% showed a supportive attitude towards the LWV policy. Results from the structural equational modeling showed a positive correlation between resilient coping and self-efficacy. Resilient coping was associated with support towards the LWV policy directly and indirectly through a lower level of emotional distress. Self-efficacy had a direct association with support towards the LWV policy but its indirect association through emotional distress was not significant. Interventions that foster resilient coping and self-efficacy would be effective in reducing public emotional distress and promoting their positive view towards the LWV policy.
“We Are Not Different than Others”: A Qualitative Study of the Lived Experience of Hispanic Adolescents and Young Adults Living with Perinatally Acquired HIV
Authors: Fair Cynthia , Fraser Leandra , Zendt Mackenzie , Santana-Garces Maria , Homans James , Stek Alice , Operskalski Eva
Published: February 2023

Though Hispanic youth with perinatally acquired HIV (PHIV) comprise 14% of those living with PHIV, little research has documented their lived experiences. Eighteen Hispanic adolescents and young adults (AYA) with PHIV were recruited from two pediatric infectious disease clinics in California (mean age = 20.8 years, 12 females and 6 males). Interview transcripts were analyzed for emergent themes regarding relationships, childbearing intentions, and future career aspirations. Participants acknowledged HIV as cause for rejection and fear of transmission from partners. Most desired children in the future. Those with children (n = 7) expressed a strong desire to continue their education for the benefit of their children. Many did not view HIV as a barrier to their career aspirations. HIV influenced their daily lives. However, the challenges of poverty, loss, and trauma also significantly shaped their well-being. Health care providers offered emotional and instrumental support which helped AYA make progress towards their goals.
A Multi-Component Educational Intervention for Addressing Levels of Physical Activity and Sedentary Behaviors of Schoolchildren
Authors: Vilardell-Dávila Ana , Martínez-Andrade Gloria , Klünder-Klünder Miguel , Miranda-Lora América Liliana , Mendoza Eugenia , Flores-Huerta Samuel , Vargas-González Jorge Eduardo , Duque Ximena , Vilchis-Gil Jenny
Published: February 2023

Physical inactivity and a sedentary lifestyle are risk factors for excess weight and obesity in childhood. It is, therefore, necessary to adopt strategies which can modify these behaviors during childhood, the age at which habits are formed. This study aimed to evaluate the impact of an educational intervention using digital media and face-to-face activities involving children, parents, and the school community on the level of physical activity and sedentary behavior among schoolchildren. This was a secondary analysis of data obtained from a community trial in which students from four primary schools in Mexico City participated. Two schools were assigned to the intervention group (IG) and two to the control group (CG). The intervention lasted 12 months and included a face-to-face component, which involved sessions and workshops for parents and children, as well as visual material for children and a distance component utilizing electronic means (web portal and text messages to mobile phones) for parents. Anthropometric measurements were taken and information was collected on moderate to vigorous physical activity performed by the children and on the time that the schoolchildren spent in front of screens at the beginning of the study and at 6 and 12 months. Information on 201 children from the IG and 167 children from the CG was included in the analysis. At 12 months, the IG showed a mean decrease of 33.4 min/d [95% CI: −53.5 to −13.3] in screen time, while the CG showed an increase of 12.5 min/d [CI 95%: −10.5 to 35.6], p = 0.003. After 12 months of follow-up, applying this educational intervention reduced the time that schoolchildren spent in front of screens. Educational intervention is a feasible and accessible strategy for promoting changes in sedentary behaviors in the school-age population.
Ethnic-Regional Differences in the Allocation of High Complexity Spending in Brazil: Time Analysis 2010–2019
Authors: Martins Luiz Oscar Machado , dos Reis Marcio Fernandes , Chaoubah Alfredo , Rego Guilhermina
Published: February 2023

The following paper presents as a research problem the ethnic-regional differences in the allocation of high complexity spending in Brazil in an analysis from 2010 to 2019. This is a descriptive research in which a generalized linear model (GLM) was developed to analyze these hospital expenditures with high complexity procedures. The total spending on high complexity procedures in Brazil has increased over the past decade. The study shows that the lowest average expenditures are found in the North and Northeast regions. When comparing the spending between different ethnicities, it was observed that the only decrease between the years 2010 and 2019 was in the amount spent on procedures in indigenous people. The spending on male patients was significantly higher compared to female patients. The highest expenditures, on the other hand, are concentrated in the regions of state capitals favoring the strengthening of hub municipalities. Geographic inequalities in access still persist, even with most states already offering almost all procedures. The Brazilian territory is very heterogeneous and needs to organize its health system by regions, therefore integrated public policies and economic and social development are urgently needed.
Acceptance of a Mobile Telepresence Robot, before Use, to Remotely Supervise Older Adults’ Adapted Physical Activity
Authors: Mascret Nicolas , Temprado Jean-Jacques
Published: February 2023

Many older adults remain sedentary because they do not have access to specialized facilities or adapted physical activity (APA) teachers. To solve this health issue, mobile telepresence robots (MTRs) could allow APA sessions to be supervised by a teacher from a distant location. However, their acceptance has never been investigated in the context of APA. A sample of 230 French older adults filled out a questionnaire assessing the variables of the Technology Acceptance Model and their expectations for aging. The results showed that the more the older adults found the MTR useful, easy to use, enjoyable, and recommended by their entourage, the more they intended to use it. Moreover, older adults who expected higher health-related quality of life with aging are those who found the MTR more useful. Finally, older adults significantly found the MTR useful, easy to use, and pleasant to use for remote supervision of their physical activity.
Causal Relationships between Daily Physical Activity, Physical Function, and Cognitive Function Ultimately Leading to Happiness
Authors: Inada Yuna , Tohda Chihiro
Published: February 2023

Frailty is a common age-related condition linked with mobility disorders, long-term care, and death. To prevent frailty, physical activities are considered effective. Several studies have indicated that physical activity can influence mental health as well as body function. Physical activity, cognitive function, and subjective mental health must relate to each other. However, most studies only focus on one-to-one interactions. This observational study aims to clarify the overall relationship and causality between subjective mental health, daily physical activity, and physical and cognitive functions. We recruited 45 people (24 males and 21 females) over 65 years old. Participants visited the university twice and were subjected to activity measurements at home. To examine the causal relationships and related structures between the indicators, structural equation modeling was performed. The results suggest that daily physical activity explains physical function, physical function explains cognitive function, and cognitive function explains subjective mental health, quality of life, and happiness. This study is the first to clarify interactive relationships as an axis that start from daily physical activity to happiness in older adults. Upregulating daily physical activity may improve physical and cognitive functions as well as mental health; this might protect and ameliorate physical, mental, and social frailties.
Personalized Care for Patients with Cancer in the Precision-Medicine Era
Authors: Lopes-Júnior Luís Carlos , Veronez Luciana Chain
Published: February 2023

Important advances in cancer management have been made in the beginning of the 21st century [...]
Estimation of Travel Cost between Geographic Coordinates Using Artificial Neural Network: Potential Application in Vehicle Routing Problems
Authors: Lee Keyju , Chae Junjae
Published: February 2023

The vehicle routing problem (VRP) attempts to find optimal (minimum length) routes for a set of vehicles visiting a set of locations. Solving a VRP calls for a cost matrix between locations. The size of the matrix grows quadratically with an increasing number of locations, restricting large-sized VRPs from being solved in a reasonable amount of time. The time needed to obtain a cost matrix is expensive when routing engines are used, which solve shortest path problems in the back end. In fact, details on the shortest path are redundant; only distance or time values are necessary for VRPs. In this study, an artificial neural network (ANN) that receives two geo-coordinates as input and provides estimated cost (distance and time) as output is trained. The trained ANN model was able to estimate with a mean absolute percentage error of 7.68%, surpassing the quality of 13.2% with a simple regression model on Euclidean distance. The possibility of using a trained model in VRPs is examined with different implementation scenarios. The experimental results with VRPs confirm that using ANN estimation instead of Euclidean distance produces a better solution, which is verified to be statistically significant. The results also suggest that an ANN can be a better choice than routing engines when the trade-off between response time and solution quality is considered.
Identifying the Gut Virome of Diaphorina citri from Florida Groves
Authors: Lin Chun-Yi , Batuman Ozgur , Levy Amit
Published: February 2023

Asian citrus psyllid (Diaphorina citri) transmits the bacterial pathogen Candidatus Liberibacter asiaticus (CLas), the putative causative agent of citrus Huanglongbing disease (HLB). Insect-specific viruses can act against insects as their natural enemies, and recently, several D. citri-associated viruses were discovered. The insect gut plays an important role as not only a pool for diverse microbes but also as a physical barrier to prevent the spread of pathogens such as CLas. However, there is little evidence of the presence of D. citri-associated viruses in the gut and of the interaction between them and CLas. Here, we dissected psyllid guts collected from five growing regions in Florida, and the gut virome was analyzed by high throughput sequencing. Four insect viruses, including D. citri-associated C virus (DcACV), D. citri densovirus (DcDV), D. citri reovirus (DcRV), and D. citri flavi-like virus (DcFLV), were identified, and their presence in the gut, including an additional D. citri cimodo-like virus (DcCLV), were confirmed with PCR-based assays. Microscopic analysis showed that DcFLV infection leads to morphological abnormalities in the nuclear structure in the infected psyllid gut cells. The complex and diverse composition of microbiota in the psyllid gut suggests a possible interaction and dynamics between CLas and the D. citri-associated viruses. Our study identified various D. citri-associated viruses that localized in the psyllid gut and provided more information that helps to evaluate the potential vectors for manipulating CLas in the psyllid gut.
Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness
Authors: Lee Hyungbin , Bae Allen , Kim John , Kingsley Karl
Published: February 2023

Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability. Using an existing DPSC repository, n = 16 DPSC isolates were cultured and 96-well growth assays were performed, which revealed FN, LN5 and the combination of these were sufficient to induce statistically significant changes in growth among five (n = 5) DPSC isolates. In addition, the administration of FN (either alone or in combination) was sufficient to induce the expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), while LN5 induced the expression of ALP only, suggesting differential responsiveness among DPSCs. Moreover, these responses appeared to correlate with the expression of MSC biomarkers NANOG, Oct4 and Sox2. These results add to the growing body of evidence suggesting that functional biomaterials, such as ECM glycoproteins FN and LN5, are sufficient to induce phenotypic and differentiation-specific effects in a specific subset of DPSC isolates. More research will be needed to determine which biomarkers or additional factors are necessary and sufficient to induce the differentiation and development of DPSCs ex vivo and in vitro for biomedical applications.
A Review on Wire-Fed Directed Energy Deposition Based Metal Additive Manufacturing
Authors: Özel Tuğrul , Shokri Hamed , Loizeau Raphaël
Published: February 2023

Metal additive manufacturing has reached a level where products and components can be directly fabricated for applications requiring small batches and customized designs, from tinny body implants to long pedestrian bridges over rivers. Wire-fed directed energy deposition based additive manufacturing enables fabricating large parts in a cost-effective way. However, achieving reliable mechanical properties, desired structural integrity, and homogeneity in microstructure and grain size is challenging due to layerwise-built characteristics. Manufacturing processes, alloy composition, process variables, and post-processing of the fabricated part strongly affect the resultant microstructure and, as a consequence, component serviceability. This paper reviews the advances in wire-fed directed energy deposition, specifically wire arc metal additive processes, and the recent efforts in grain tailoring during the process for the desired size and shape. The paper also addresses modeling methods that can improve the qualification of fabricated parts by modifying the microstructure and avoid repetitive trials and material waste.
Vitamin A, Vitamin E, and Carotenoid Concentrations in Livers of Marine Toads (Rhinella marina)
Authors: Lee Hanna , Minter Larry J. , Tollefson Troy N. , Ridgley Frank , Smith Dustin , Treiber Kimberly , Scott Heather , Troan Brigid V. , Ange-van Heugten Kimberly D.
Published: February 2023

Global amphibian population decline is a major concern. Therefore, conservation centers have become increasingly needed for population sustainability breeding programs. This need makes proper nutrition programs while in human care imperative. The specific nutritional focus of this current research was to analyze vitamin A, vitamin E, and carotenoid (apocarotenoid, β-carotene, β-carotene ester, β-cryptoxanthin, lutein, lutein ester, zeaxanthin, and zeaxanthin ester) concentrations in the liver of 66 free-range marine toads (Rhinella marina) over a two-month period after entering human management. Toads were fed supplemented crickets randomly assigned to one of two diets: Diet 1 consisted of brown house crickets (Acheta domestica) gut loaded with Mazuri® Cricket Diet 5M38 and small amounts of sweet potato and carrots; Diet 2 consisted of an identical diet with the gut loaded crickets additionally dusted with Repashy® Superfoods Vitamin A Plus. Ten toads were euthanized prior to human management on Day 0 to assess baseline liver nutrients. Seven toads consuming each of the two diets (14 toads total) were euthanized on Days 9, 15, 32, and 62. Regardless of diet treatment, there were decreases (p < 0.05) in all the analyzed nutrient concentrations over the 62-day human management period. The results from this study indicate that higher dietary vitamin A, vitamin E, and carotenoid content may be needed and/or the cricket gut loading and dusting techniques used to feed the amphibians food source insects may be inadequate.
Effect of Spatial Characteristics of Farmland Plots on Transfer Patterns in China: A Supply and Demand Perspective
Authors: Guo Yang , Cui Meiling , Xu Zhigang
Published: February 2023

(1) Background: The tense relationship between man and land makes transferring farmland rights in the market critical for improving agricultural production efficiency and promoting large-scale agricultural management. (2) Methods: This study considers the impact of the spatial characteristics of farmland plots on the economies of scale of farmers in terms of farmland use and heterogeneity. The effect of plots’ area and location on the directional flow of plots in the farmland transfer market from the perspective of matching supply and demand is also investigated. An empirical test is conducted on farmer actions and plot characteristics data based on surveys from 2015 and 2018 in the Chinese provinces of Heilongjiang, Henan, Zhejiang, and Sichuan. (3) Results: The plots’ area and location affect economies of scale for different potential transfer plots. This leads to large plots and adjacent plots in the market transferring to large-scale households, while scattered small plots mainly transfer to ordinary households. (4) Conclusions: The fixed spatial characteristics of the plots determine the scattered circulation of farmland in the transfer market, hindering the centralized utilization of farmland and restricting efficiency in farmland transfer market allocation. The findings from the context of China are similar to what has been found elsewhere. This suggests the need for a unified trading platform for farmland transfer and strengthening the mutual transformation of land and agricultural machinery.
Improvement of Frictional Property of AISI D2 Tool Steel Surface against JIS SPFC 980Y Advanced High-Strength Steel by Using Laser Texturing Process
Authors: Daodon Witthaya , Saetang Viboon
Published: February 2023

Surface friction in metal forming processes can be reduced by creating lubricant reservoirs at the interface between surfaces in contact, and a laser texturing process can be employed to produce the micro-dimples that act as the reservoirs on the surfaces. However, the role of the laser texturing parameters in the friction reduction of tool steel surfaces has still received very little attention. Therefore, this study aims to reduce the friction of the AISI D2 tool steel surface on which a nanosecond pulse laser was applied to create an array of micro-lubricant pockets for trapping lubricant. The effects of laser power, irradiation duration, and spacing distance between pockets on the pocket diameter, size of the heat-affected zone, surface friction, and wettability were investigated in this work. The average laser power in the range from 5 to 10 W and laser irradiation duration of 0.02 to 0.10 s were applied. The results showed that the increase in laser power and irradiation duration enlarged the pocket diameter and heat-affected zone. The largest pocket diameter of 40 µm was achievable by using 10 W laser power together with 0.10 s irradiation time. The pin-on-disc method was employed to determine the friction coefficient of the tool steel, where JIS SPFC 980Y advanced high-strength steel was used as a disc. The friction coefficient of laser-textured with different spacing distances of 150, 200, and 250 µm versus untextured surfaces was compared and found to vary depending on the applied normal load. The laser-textured surface having a pocket spacing distance of 150 µm and pocket density of 5.6%, offered the lowest friction coefficient of 0.097 on average for all tested loads, whereas the average friction coefficient of the untextured surface was 0.117. In addition, the wettability of textured surfaces was insignificantly changed compared to that of untextured ones, so the micro-lubricant pockets did not cause oleophobicity affecting the performance of lubrication. Well-defined micro-pockets using the most appropriate laser parameters, i.e., 10 W laser power with 0.10 s irradiation duration and 150 µm spacing distance, successfully reduced the sliding friction of contacting couples between the laser-textured tool steel and advanced high-strength steel surfaces. The low surface friction induced by the laser-fabricated micro-lubricant pockets has been feasible for the forming tool and die applications where the energy consumed in their operations can be minimized.
Basosquamous Cell Carcinoma of the Nipple-Areola Complex—Report of a Case
Authors: Raimondo Gabriele , Gallo Gaetano , D’Onghia Giuliano , Gabriele Giovanni , Izzo Luciano , Polistena Andrea , Esposito Luca , Giancontieri Paola , Macci Leonardo , D’Andrea Vito , Fiori Enrico , Basso Luigi
Published: February 2023

Basosquamous cell carcinoma (BSCC) is a rare malignancy usually arising on sun-exposed areas of the skin. BSCC is described as a rare variant of Basal cell carcinoma (BCC) which shows clinical and microscopic features of both BCC and of Squamous cell carcinoma (SCC). We report the case of a 70-year-old male with a cutaneous lesion of the nipple-areola complex (NAC); to the best of our knowledge, this is the first ever reported patient with BSCC in this area. The lesion had a fast growth, but, due to the COVID19 crisis, the patient only came to our observation one year after onset of this condition. Physical examination showed a bleeding red ulcerated lesion that involved the NAC, measuring 27 mm × 20 mm. Biopsy showed a BSCC. Pre-operative breast ultrasound scan, mammogram and MRI were all performed before surgery, which consisted of simple mastectomy and sentinel lymph-node biopsy. The patient was discharged home on the 4th post-operative day, and at 18-month follow-up there are no signs or clinical evidence of local recurrence or metastases. Diagnosis of BSCC of the nipple-areola complex requires high index of suspicion and a thorough differential diagnosis, management, and suitable radical treatment due to well described high rates of recurrence and of metastases. Differential diagnosis with similar lesions (e.g., Paget’s disease, Bowen’s disease, BCC, and SCC) should also be taken into account.
Effects of a Pseudomonas Strain on the Lipid Transfer Proteins, Appoplast Barriers and Activity of Aquaporins Associated with Hydraulic Conductance of Pea Plants
Authors: Martynenko Elena , Arkhipova Tatiana , Akhiyarova Guzel , Sharipova Guzel , Galin Ilshat , Seldimirova Oksana , Ivanov Ruslan , Nuzhnaya Tatiana , Finkina Ekaterina , Ovchinnikova Tatiana , Kudoyarova Guzel
Published: February 2023

Lipid transfer proteins (LTPs) are known to be involved in suberin deposition in the Casparian bands of pea roots, thereby reinforcing apoplast barriers. Moreover, the Pseudomonas mandelii IB-Ki14 strain accelerated formation of the Casparian bands in wheat plants, although involvement of LTPs in the process was not studied. Here, we investigated the effects of P. mandelii IB-Ki14 on LTPs, formation of the Casparian bands, hydraulic conductance and activity of aquaporins (AQPs) in pea plants. RT PCR showed a 1.6-1.9-fold up-regulation of the PsLTP-coding genes and an increase in the abundance of LTP proteins in the phloem of pea roots induced by the treatment with P. mandelii IB-Ki14. The treatment was accompanied with increased deposition of suberin in the Casparian bands. Hydraulic conductance did not decrease in association with the bacterial treatment despite strengthening of the apoplast barriers. At the same time, the Fenton reagent, serving as an AQPs inhibitor, decreased hydraulic conductance to a greater extent in treated plants relative to the control group, indicating an increase in the AQP activity by the bacteria. We hypothesize that P. mandelii IB-Ki14 stimulates deposition of suberin, in the biosynthesis of which LTPs are involved, and increases aquaporin activity, which in turn prevents a decrease in hydraulic conductance due to formation of the apoplast barriers in pea roots.
Modification of the Cu/W Interface Cohesion by Segregation
Authors: Bodlos Rishi , Scheiber Daniel , Spitaler Jürgen , Romaner Lorenz
Published: February 2023

Cu/W composites are widely used in various industrial fields as they show thermomechanical properties suitable for a wide range of applications. Additionally, in semiconductor products, WTi in contact with Cu acts as a barrier material between Cu and Si/SiO2. Therefore, the bonding behavior of both Cu/W and Cu/WTi is of great economical interest, also with respect to the effects that impurities could have on the behaviour of the Cu/W(Ti) interface. The segregation behavior of relevant impurities has not been studied in detail before. In this work, we create atomistic models of the Cu/W and Cu/WTi interfaces, compare their energetics to previously known interfaces and study the effect of segregation on the interface cohesion. We find that all investigated segregants, i.e. Ti, Cl, S, Al, H, O, and vacancies weaken the cohesion of the interface.
Metabolomics-Based Mechanistic Insights into Revealing the Adverse Effects of Pesticides on Plants: An Interactive Review
Authors: Shahid Mohammad , Singh Udai B. , Khan Mohammad Saghir
Published: February 2023

In plant biology, metabolomics is often used to quantitatively assess small molecules, metabolites, and their intermediates in plants. Metabolomics has frequently been applied to detect metabolic alterations in plants exposed to various biotic and abiotic stresses, including pesticides. The widespread use of pesticides and agrochemicals in intensive crop production systems is a serious threat to the functionality and sustainability of agroecosystems. Pesticide accumulation in soil may disrupt soil–plant relationships, thereby posing a pollution risk to agricultural output. Application of metabolomic techniques in the assessment of the biological consequences of pesticides at the molecular level has emerged as a crucial technique in exposome investigations. State-of-the-art metabolomic approaches such as GC–MS, LC–MS/MS UHPLC, UPLC–IMS–QToF, GC/EI/MS, MALDI-TOF MS, and 1H-HR-MAS NMR, etc., investigating the harmful effects of agricultural pesticides have been reviewed. This updated review seeks to outline the key uses of metabolomics related to the evaluation of the toxicological impacts of pesticides on agronomically important crops in exposome assays as well as bench-scale studies. Overall, this review describes the potential uses of metabolomics as a method for evaluating the safety of agricultural chemicals for regulatory applications. Additionally, the most recent developments in metabolomic tools applied to pesticide toxicology and also the difficulties in utilizing this approach are discussed.
Fluorescence Sensing of Some Important Nitroaromatic Compounds by Using Polyaniline Ag Composite
Authors: Ture Satish Ashok , Pattathil Shruthy D. , Zing Bertrand Zing , Abbaraju Venkataraman
Published: February 2023

Conducting polymers (CPs) have contributed significantly to the field of sensing. The sensing of nitroaromatic compounds by fluorescence has recently gained more attention due to its sensitivity and selectivity. In this study, polyaniline (PANI) was functionalized by forming a polyaniline-Ag (PANI-Ag) composite and used as a fluorophore for sensing. The nitro groups present in nitroaromatic compounds (NACs) such as 2,4,6-trinitrophenol (picric acid-TNP) and Dinitrobenzene (DNB) act as electron-accepting molecules and quench the fluorescence of polymer chains by showing an amplified quenching effect in which trace amounts of electron-accepting NACs quench emissions of several fluorophore units. The PANI-Ag composite synthesized by interfacial polymerization was analyzed using UV-vis spectroscopy and Fourier-transform infrared (FTIR) spectroscopy for determination of molecular structure; X-ray powder diffraction (XRD) and scanning electron microscopy (SEM/EDAX) for its morphology, which is cubic crystalline silver; and thermogravimetric analysis (TGA) for the thermal stability. The fluorescence quenching mechanism was deduced from the Stern–Volmer plot. The quenching constant value (Ksv) obtained from the Stern–Volmer (S–V) plot was found to be Ksv = 0.1037 × 106 M−1 (TNP) and Ksv = 0.161 × 104 M−1 (DNB). The plot shows a single mechanism with formation of an exciplex complex for TNP with a photoinduced electron transfer (PET) mechanism. The limit of detection (LOD) is found to be TNP = 5.58 × 10−7 M, whereas DNB = 23.30 × 10−6 M shows that the PANI-Ag composite is a potential fluorophore for sensing of nitroaromatic compounds in trace levels.
Application of Clay Materials for Sorption of Radionuclides from Waste Solutions
Authors: Singh Bhupendra Kumar , Um Wooyong
Published: February 2023

The wide application of nuclear resources in various fields has resulted in the production of radioactive waste, which poses a serious threat to lives and the environment. Nuclear waste contains long-lived radionuclides and, due to its mobility in environments, the proper management of generated waste is necessary. To impede the mobility of radionuclides in environments, various materials have been tested as suitable sorbents under different experimental conditions. In this review, we thoroughly discuss some key and recent contributions to the application of natural clays (NCs) and modified/functionalized clays (MCs) for the sorption of various radionuclides in their cationic and anion forms from (simulated) waste solutions under different experimental conditions. More specifically, we discuss the key developments toward the use of natural clays for the efficient sorption of various radioactive contaminates. Later, this review targets the modification/functionalization of natural clays using various organic moieties to improve their removal capacities for various radionuclides/hazardous ions present in waste solutions. Finally, we summarize the major aspects and highlight the key challenges to be addressed in future studies to further enhance the application of clays and clay-based materials for selective and effective removal of various radionuclides from waste solutions.
Peptidic Inhibitors and a Fluorescent Probe for the Selective Inhibition and Labelling of Factor XIIIa Transglutaminase
Authors: Gates Eric W. J. , Mansour Kian , Ebrahimi Samani Sahar , Shad Sammir , Kaartinen Mari T. , Keillor Jeffrey W.
Published: February 2023

Factor XIIIa (FXIIIa) is a transglutaminase of major therapeutic interest for the development of anticoagulants due to its essential role in the blood coagulation cascade. While numerous FXIIIa inhibitors have been reported, they failed to reach clinical evaluation due to their lack of metabolic stability and low selectivity over transglutaminase 2 (TG2). Furthermore, the chemical tools available for the study of FXIIIa activity and localization are extremely limited. To combat these shortcomings, we designed, synthesised, and evaluated a library of 21 novel FXIIIa inhibitors. Electrophilic warheads, linker lengths, and hydrophobic units were varied on small molecule and peptidic scaffolds to optimize isozyme selectivity and potency. A previously reported FXIIIa inhibitor was then adapted for the design of a probe bearing a rhodamine B moiety, producing the innovative KM93 as the first known fluorescent probe designed to selectively label active FXIIIa with high efficiency (kinact/KI = 127,300 M−1 min−1) and 6.5-fold selectivity over TG2. The probe KM93 facilitated fluorescent microscopy studies within bone marrow macrophages, labelling FXIIIa with high efficiency and selectivity in cell culture. The structure–activity trends with these novel inhibitors and probes will help in the future study of the activity, inhibition, and localization of FXIIIa.
Fractioning and Compared 1H NMR and GC-MS Analyses of Lanolin Acid Components
Authors: Morano Camillo , Dei Cas Michele , Bergamaschi Roberta F. , Palmisano Erika , Pallavicini Marco , Bolchi Cristiano , Roda Gabriella , Casati Sara
Published: February 2023

The management of food and food-related wastes represents a growing global issue, as they are hard to recycle and dispose of. Foremost, waste can serve as an important source of biomasses. Particularly, fat-enriched biomasses are receiving more and more attention for their role in the manufacturing of biofuels. Nonetheless, many biomasses have been set aside over the years. Wool wax, also known as lanolin, has a huge potential for becoming a source of typical and atypical fatty acids. The main aim of this work was to evaluate and assess a protocol for the fractioning of fatty acids from lanolin, a natural by-product of the shearing of sheep, alongside the design of a new and rapid quantitative GC-MS method for the derivatization of free fatty acids in fat mixtures, using MethElute™. As the acid portion of lanolin is characterized by the presence of both aliphatic and hydroxylated fatty acids, we also evaluated a procedure for the parting of these two species, by using NMR spectroscopy, benefitting of the different solubilities of the components in organic solvents. At last, we evaluated and quantified the fatty acids and the α-hydroxy fatty acids present in each attained portion, employing both analytical and synthetic standards. The performed analyses, both qualitative and quantitative, showed a good performance in the parting of the different acid components, and GC-MS allowed to speculate that the majority of α-hydroxylated fatty acids is formed of linear saturated carbon chains, while the totality of properly said fatty acids has a much more complex profile.
Effect of Europium Addition on the Microstructure and Dielectric Properties of CCTO Ceramic Prepared Using Conventional and Microwave Sintering
Authors: Evangeline T Gecil , Annamalai A Raja , Ctibor Pavel
Published: February 2023

In this work, Eu2O3-doped (CaCu3Ti4O12)x of low dielectric loss have been fabricated using both conventional (CS) and microwave sintering (MWS), where x = Eu2O3 = 0.1, 0.2, and 0.3, respectively. According to X-ray diffraction (XRD) and scanning electron microscope (SEM) reports, increasing the concentration of Eu3+ in the CCTO lattice causes the grain size of the MWS samples to increase and vice versa for CS. The X-ray photoelectron spectroscopy (XPS) delineated the binding energies and charge states of the Cu2+/Cu+ and Ti4+/Ti3+ transition ions. Energy dispersive spectroscopy (EDS) analysis revealed no Cu-rich phase along the grain boundaries that directly impacts the dielectric properties. The dielectric characteristics, which include dielectric constant (ε) and the loss (tan δ), were examined using broadband dielectric spectrometer (BDS) from 10 to 107 Hz at ambient temperature. The dielectric constant was >104 and >102 for CS and MWS samples at x > 0.1, respectively, with the low loss being constant even at high frequencies due to the effective suppression of tan δ by Eu3+. This ceramic of low dielectric loss has potential for commercial applications at comparatively high frequencies.
In Silico Identification of Novel Derivatives of Rifampicin Targeting Ribonuclease VapC2 of M. tuberculosis H37Rv: Rifampicin Derivatives Target VapC2 of Mtb H37Rv
Authors: Maurya Satyamvada , Jain Amita , Rehman Md Tabish , Hakamy Ali , Bantun Farkad , AlAjmi Mohamed F. , Singh Vineeta , Zehra Aafreen , Khan Feroz , Haque Shafiul , Mishra Bhartendu Nath
Published: February 2023

The emergence of multi-drug-resistant Mycobacterium tuberculosis (Mtb) strains has rendered many of the currently available anti-TB drugs ineffective. Hence, there is a pressing need to discover new potential drug targets/candidates. In this study, attempts have been made to identify novel inhibitors of the ribonuclease VapC2 of Mtb H37Rv using various computational techniques. Ribonuclease VapC2 Mtb H37Rv’s protein structure was retrieved from the PDB databank, 22 currently used anti-TB drugs were retrieved from the PubChem database, and protein–ligand interactions were analyzed by docking studies. Out of the 22 drugs, rifampicin (RIF), being a first-line drug, showed the best binding energy (−8.8 Kcal/mol) with Mtb H37Rv VapC2; hence, it was selected as a parent molecule for the design of its derivatives. Based on shape score and radial plot criteria, out of 500 derivatives designed through SPARK (Cresset®, Royston, UK) program, the 10 best RIF derivatives were selected for further studies. All the selected derivatives followed the ADME criteria concerning drug-likeness. The docking of ribonuclease VapC2 with RIF derivatives revealed the best binding energy of −8.1 Kcal/mol with derivative 1 (i.e., RIF-155841). A quantitative structure–activity relationship study revealed that derivative 1’s activity assists in the inhibition of ribonuclease VapC2. The stability of the VapC2–RIF155841 complex was evaluated using molecular dynamics simulations for 50 ns and the complex was found to be stable after 10 nsec. Further, a chemical synthesis scheme was designed for the newly identified RIF derivative (RIF-155841), which verified that its chemical synthesis is possible for future in vitro/in vivo experimental validation. Overall, this study evaluated the potential of the newly designed RIF derivatives with respect to the Mtb VapC2 protein, which is predicted to be involved in some indispensable processes of the related pathogen. Future experimental studies regarding RIF-155841, including the exploration of the remaining RIF derivatives, are warranted to verify our current findings.
Virtual Practical Examination for Student Nurse Educators in Health Sciences Education during the COVID-19 Pandemic: A Narrative Review
Authors: Matlhaba Kholofelo Lorraine
Published: February 2023

(1) Background: There is a gap in the literature that explores challenges and opportunities relating to virtual or e-assessment health science education with particular relevance to the Health Sciences Education practical examination for student nurse educators. Therefore, this review aimed to address this gap and provide recommendations for enhancing identified opportunities and for overcoming identified challenges.; (2) Methods: The review was conducted across Google Scholar, PubMed/MEDLINE, Science Direct, Directory of Open Access Journals, Complementary Index, SCOPUS, and the Cumulative Index to Nursing and Allied Health Literature (CINAHL) with the intention of identifying opportunities and challenges presented by e-assessment in the HSE practical examination for student nurse educators during the COVID-19 pandemic.; (3) Results: The following aspects are discussed: (1) opportunities, including benefits, for both student nurse educators and facilitators and opportunities for Nursing Education; and (2) challenges, including issues with accessibility and connectivity as well as the attitudes of both students and facilitators.; (4) Conclusions: Despite challenges which included connectivity issues that led to frustration and stress, the unpreparedness and attitudes of students and facilitators, there are some opportunities that have emerged from e-assessment that can be beneficial to both the students and the facilitators, as well as the institutions. These include a reduced administrative burden, improved teaching and learning, and immediate feedback from facilitators to students and from students to facilitators.
A Review on the Immunological Response against Trypanosoma cruzi
Authors: Macaluso Giusi , Grippi Francesca , Di Bella Santina , Blanda Valeria , Gucciardi Francesca , Torina Alessandra , Guercio Annalisa , Cannella Vincenza
Published: February 2023

Chagas disease is a chronic systemic infection transmitted by Trypanosoma cruzi. Its life cycle consists of different stages in vector insects and host mammals. Trypanosoma cruzi strains cause different clinical manifestations of Chagas disease alongside geographic differences in morbidity and mortality. Natural killer cells provide the cytokine interferon-gamma in the initial phases of T. cruzi infection. Phagocytes secrete cytokines that promote inflammation and activation of other cells involved in defence. Dendritic cells, monocytes and macrophages modulate the adaptive immune response, and B lymphocytes activate an effective humoral immune response to T. cruzi. This review focuses on the main immune mechanisms acting during T. cruzi infection, on the strategies activated by the pathogen against the host cells, on the processes involved in inflammasome and virulence factors and on the new strategies for preventing, controlling and treating this disease.