bluuebunny/crossref_metadata_2025 huggingface dataset:
Tourist Arrival Forecasting Using Multiscale Mode Learning Model
Authors: He Kaijian , Wu Don , Zou Yingchao
Published: August 2022

The forecasting of tourist arrival depends on the accurate modeling of prevalent data patterns found in tourist arrival, especially for daily tourist arrival, where tourist arrival changes are more complex and highly nonlinear. In this paper, a new multiscale mode learning-based tourist arrival forecasting model is proposed to exploit different multiscale data features in tourist arrival movement. Two popular Mode Decomposition models (MD) and the Convolutional Neural Network (CNN) model are introduced to model the multiscale data features in the tourist arrival data The data patterns at different scales are extracted using these two different MD models which dynamically decompose tourist arrival into the distinctive intrinsic mode function (IMF) data components. The convolutional neural network uses the deep network to further model the multiscale data structure of tourist arrivals, with the reduced dimensionality of key multiscale data features and finer modeling of nonlinearity in tourist arrival. Our empirical results using daily tourist arrival data show that the MD-CNN tourist arrival forecasting model significantly improves the forecasting reliability and accuracy.
Reinforcement Learning-Based Routing Protocols in Flying Ad Hoc Networks (FANET): A Review
Authors: Lansky Jan , Ali Saqib , Rahmani Amir Masoud , Yousefpoor Mohammad Sadegh , Yousefpoor Efat , Khan Faheem , Hosseinzadeh Mehdi
Published: August 2022

In recent years, flying ad hoc networks have attracted the attention of many researchers in industry and universities due to easy deployment, proper operational costs, and diverse applications. Designing an efficient routing protocol is challenging due to unique characteristics of these networks such as very fast motion of nodes, frequent changes of topology, and low density. Routing protocols determine how to provide communications between drones in a wireless ad hoc network. Today, reinforcement learning (RL) provides powerful solutions to solve the existing problems in the routing protocols, and designs autonomous, adaptive, and self-learning routing protocols. The main purpose of these routing protocols is to ensure a stable routing solution with low delay and minimum energy consumption. In this paper, the reinforcement learning-based routing methods in FANET are surveyed and studied. Initially, reinforcement learning, the Markov decision process (MDP), and reinforcement learning algorithms are briefly described. Then, flying ad hoc networks, various types of drones, and their applications, are introduced. Furthermore, the routing process and its challenges are briefly explained in FANET. Then, a classification of reinforcement learning-based routing protocols is suggested for the flying ad hoc networks. This classification categorizes routing protocols based on the learning algorithm, the routing algorithm, and the data dissemination process. Finally, we present the existing opportunities and challenges in this field to provide a detailed and accurate view for researchers to be aware of the future research directions in order to improve the existing reinforcement learning-based routing algorithms.
Aromatic Polyketides from the Deep-Sea Cold-Seep Mussel Associated Endozoic Fungus Talaromyces minioluteus CS-138
Authors: Song Qi , Yang Sui-Qun , Li Xiao-Ming , Hu Xue-Yi , Li Xin , Wang Bin-Gui
Published: August 2022

Five new aromatic polyketides, including a unique benzofuran derivative, talarominine A (1), and four chromone analogs talamins A–D (2–5), along with one known related metabolite, 5-hydroxy-7-methoxy-2,3-dimethylchromone (6), were isolated and identified from the Talaromyces minioluteus CS-138, an endozoic fungus obtained from the deep-sea cold seep mussel Gigantidas platifrons. Their chemical structures were elucidated by detailed analysis of their NMR spectra, HRESIMS and X-ray crystallographic data, and by comparison with literature data as well. The antibacterial and DPPH scavenging activities of compounds 1–6 were evaluated. Compounds 1–3 showed inhibitory activity against some of the tested bacteria whereas compounds 2 and 5 showed potent DPPH radical scavenging activities, which were better than that of the positive control butylated hydroxytoluene (BHT). This work is likely the first report on marine natural products of mussel-derived fungus living in cold seep environments.
Application of Green Chiral Chromatography in Enantioseparation of Newly Synthesized Racemic Marinoepoxides
Authors: Buljan Anđela , Roje Marin
Published: August 2022

Enantioseparation of the newly synthesized series of novel quinoline-2(1H)-one epoxide structures rac-6a–c and rac-8a–c, named marinoepoxides, is described. Marinoepoxide rac-6a, the key intermediate in the total synthesis of natural products marinoaziridines A and B, as well as their structural analogues, was synthesized by addition of the achiral ylide generated in situ from the sulfonium salt 5 or 7, to the carbon-oxygen double bond of the corresponding quinoline-2(1H)-one-4-carbaldehyde 4a–c in good yield. Separation of enantiomers of (±)-2,3,3-trisubstituted marinoepoxides rac-6a–c and (±)-trans-2,3-disubstituted marinoepoxides rac-8a–c was studied using two immobilized polysaccharide type chiral stationary phases (CSPs); tris-(3,5-dichlorophenylcarbamoyl)cellulose stationary phase (CHIRAL ART Cellulose-SC) and tris-(3,5-dimethylphenylcarbamoyl)amylose stationary phase (CHIRAL ART Amylose-SA). Enantioseparation conditions were explored by high-performance liquid chromatography (HPLC) using dimethyl carbonate/alcohol mixtures and n-hexane/ethanol (80/20, v/v) as mobile phase, and by supercritical fluid chromatography (SFC) using CO2/alcohol mixtures as mobile phase. In all examined racemates, enantioseparation was successfully achieved, but its efficiency largely depended on the structure of chiral selector and type/composition of the mobile phase.
Discovery of Ircinianin Lactones B and C—Two New Cyclic Sesterterpenes from the Marine Sponge Ircinia wistarii
Authors: Majer Thomas , Bhattarai Keshab , Straetener Jan , Pohlmann Justus , Cahill Patrick , Zimmermann Markus O. , Hübner Marc P. , Kaiser Marcel , Svenson Johan , Schindler Michael , Brötz-Oesterhelt Heike , Boeckler Frank M. , Gross Harald
Published: August 2022

Two new ircinianin-type sesterterpenoids, ircinianin lactone B and ircinianin lactone C (7 and 8), together with five known entities from the ircinianin compound family (1, 3–6) were isolated from the marine sponge Ircinia wistarii. Ircinianin lactones B and C (7 and 8) represent new ircinianin terpenoids with a modified oxidation pattern. Despite their labile nature, the structures could be established using a combination of spectroscopic data, including HRESIMS and 1D/2D NMR techniques, as well as computational chemistry and quantum-mechanical calculations. In a broad screening approach for biological activity, the class-defining compound ircinianin (1) showed moderate antiprotozoal activity against Plasmodium falciparum (IC50 25.4 μM) and Leishmania donovani (IC50 16.6 μM).
Adult Respiratory Syncytial Virus Infection and Hypoxic Cardiac Arrest—Coexistent or Causal? A Hypothesis-Generating Case Report
Authors: Schnaubelt Sebastian , Eibensteiner Felix , Merrelaar Marieke , Tihanyi Daniel , Strassl Robert , Clodi Christian , Domanovits Hans , Losert Heidrun , Holzer Michael
Published: August 2022

Respiratory syncytial virus (RSV) is a well-known pathogen in paediatric patients. However, it also causes substantial morbidity and mortality in adults, posing a major healthcare problem. We present a patient with chronic pulmonary conditions and an acute RSV infection, thus leading to cardiac arrest (CA). We speculate that RSV as the causative agent for CA should be considered in post-resuscitation care. From a wider public health perspective, immuno-naivety for RSV caused by the coronavirus disease 2019 pandemic may induce a severe rise in cases, morbidity, and mortality in the future.
Preconception Counseling in Patients with Hypothyroidism and/or Thyroid Autoimmunity
Authors: Țarnă Mihaela , Cima Luminița Nicoleta , Panaitescu Anca Maria , Martin Carmen Sorina , Sîrbu Anca Elena , Barbu Carmen Gabriela , Pavel Bogdan , Șerbănică Andreea Nicoleta , Fica Simona
Published: August 2022

Preconception counseling is an essential tool for preventing adverse pregnancy outcomes associated with thyroid dysfunction. The high prevalence of thyroid disease among women of reproductive age, and the increased risk of adverse pregnancy outcomes associated with thyroid dysfunction, emphasize the necessity for well-established screening and treatment criteria in the preconception period. We therefore conducted a literature review for relevant information on the screening, diagnosis and treatment of subclinical and overt hypothyroidism in women seeking pregnancy. While screening for thyroid disease is recommended only in the presence of risk factors, iodine supplementation should be recommended in most regions, with higher doses in areas with severe deficiency. Known hypothyroid women should be counseled about increasing their levothyroxine dose by 20–30% in the case of suspected or confirmed pregnancy (missed menstrual cycle or positive pregnancy test). Treating subclinical hypothyroidism appears to be beneficial, especially in the presence of autoimmunity or in patients undergoing artificial reproductive techniques. Regarding the management of TPOAb negative SCH women or euthyroid women with positive TPOAb, further research is necessary in order to make evidence-based recommendations.
Natural Course of Early Detected Acute Peripancreatic Fluid Collection in Moderately Severe or Severe Acute Pancreatitis
Authors: Lee Dong Wook , Kim Ho Gak , Cho Chang Min , Jung Min Kyu , Heo Jun , Cho Kwang Bum , Kim Sung Bum , Kim Kook Hyun , Kim Tae Nyeun , Han Jimin , Kim Hyunsoo
Published: August 2022

Background and Objectives: Acute peripancreatic fluid collection (APFC) is an acute local complication of acute pancreatitis (AP) according to the revised Atlanta classification. Sometimes APFC resolves completely, sometimes it changes into a pseudocyst or walled-off necrosis (WON), so called late complications. The aim of this study is to investigate the natural course of APFC detected on early computed tomography (CT) in moderately severe (MSAP) or severe AP (SAP). Materials and Methods: From October 2014 to September 2015, patients with MSAP or SAP were enrolled if there was APFC within 48 h of onset on imaging studies at six medical centers. The status of fluid collection was followed 4–8 weeks after onset. Initial laboratory findings, CT findings and clinical scoring systems were analyzed. Results: A total of 68 patients were enrolled and APFC was completely resolved in 32 (66.7%) patients in the MSAP group and 9 (34.6%) in the SAP group. Patients with a high bedside index for severity in acute pancreatitis (BISAP) score (≥3 points) were common in the SAP group. C-reactive protein (CRP) after 48 h from admission and BUN level were also high in the SAP group. In multivariate analysis, BISAP score (≥3 points), elevation of CRP after 48 h (≥150 mg/L) and nasojejunal feeding after 48 h were risk factors for the development of late complications. Conclusions: Spontaneous resolution of APFC was more common in MSAP group and APFC can be changed to pseudocyst or WON in patients with elevated BISAP score, CRP level after 48 h, and non-improved abdominal pain.
PB1F2 from Influenza A Virus Regulates the Interaction between Cytochrome C and Cardiolipin
Authors: Wang Yujuan , Wang Junfeng
Published: August 2022

PB1F2 is a membrane associated protein encoded by the influenza virus gene in the host. Similar to endogenous pro-apoptotic proteins, it acts on the mitochondria of the host immune cells, inducing apoptosis of the cells. The PB1F2 protein has been demonstrated to facilitate the release of cytochrome c in addition to impairing the integrity of the inner mitochondrial membrane. This investigation focused on how the protein PB1F2 interacted with cardiolipin and cytochrome c. The regulation of PB1F2 on the binding of cytochrome c to cardiolipin in two kinds of in vitro membrane mimics was investigated by biophysical techniques. PB1F2 aids in the dissociation of cytochrome c-cardiolipin complexes in liposomes and nanodiscs. The results provide novel explanations and evidence for how PB1F2 functions as a viral virulence factor by inducing immune cell death.
Design of Enzyme Loaded W/O Emulsions by Direct Membrane Emulsification for CO2 Capture
Authors: Mondal Suchintan , Alke Bhavna , de Castro Aline Machado , Ortiz-Albo Paloma , Syed Usman Taqui , Crespo João G. , Brazinha Carla
Published: August 2022

Membrane-based gas separation is a promising unit operation in a low-carbon economy due to its simplicity, ease of operation, reduced energy consumption and portability. A methodology is proposed to immobilise enzymes in stable water-in-oil (W/O) emulsions produced by direct membrane emulsification systems and thereafter impregnated them in the pores of a membrane producing emulsion-based supported liquid membranes. The selected case-study was for biogas (CO2 and CH4) purification. Upon initial CO2 sorption studies, corn oil was chosen as a low-cost and non-toxic bulk phase (oil phase). The emulsions were prepared with Nadir® UP150 P flat-sheet polymeric membranes. The optimised emulsions consisted of 2% Tween 80 (w/w) in corn oil as the continuous phase and 0.5 g.L−1 carbonic anhydrase enzyme with 5% PEG 300 (w/w) in aqueous solution as the dispersed phase. These emulsions were impregnated onto a porous hydrophobic PVDF membrane to prepare a supported liquid membrane for gas separation. Lastly, gas permeability studies indicated that the permeability of CO2 increased by ~15% and that of CH4 decreased by ~60% when compared to the membrane without carbonic anhydrase. Thus, a proof-of-concept for enhancement of CO2 capture using emulsion-based supported liquid membrane was established.
Adsorptive Membrane for Boron Removal: Challenges and Future Prospects
Authors: Mehanathan Shaymala , Jaafar Juhana , Nasir Atikah , Rahman Roshanida , Ismail Ahmad , Illias Rosli , Othman Mohd , A Rahman Mukhlis , Bilad Muhammad , Naseer Muhammad
Published: August 2022

The complexity of removing boron compounds from aqueous systems has received serious attention among researchers and inventors in the water treating industry. This is due to the higher level of boron in the aquatic ecosystem, which is caused by the geochemical background and anthropogenic factors. The gradual increase in the distribution of boron for years can become extremely toxic to humans, terrestrial organisms and aquatic organisms. Numerous methods of removing boron that have been executed so far can be classified under batch adsorption, membrane-based processes and hybrid techniques. Conventional water treatments such as coagulation, sedimentation and filtration do not significantly remove boron, and special methods would have to be installed in order to remove boron from water resources. The blockage of membrane pores by pollutants in the available membrane technologies not only decreases their performance but can make the membranes prone to fouling. Therefore, the surface-modifying flexibility in adsorptive membranes can serve as an advantage to remove boron from water resources efficiently. These membranes are attractive because of the dual advantage of adsorption/filtration mechanisms. Hence, this review is devoted to discussing the capabilities of an adsorptive membrane in removing boron. This study will mainly highlight the issues of commercially available adsorptive membranes and the drawbacks of adsorbents incorporated in single-layered adsorptive membranes. The idea of layering adsorbents to form a highly adsorptive dual-layered membrane for boron removal will be proposed. The future prospects of boron removal in terms of the progress and utilization of adsorptive membranes along with recommendations for improving the techniques will also be discussed further.
Extraction and Characterization of Antioxidant Compounds in Almond (Prunus amygdalus) Shell Residues for Food Packaging Applications
Authors: Valdés Arantzazu , Garrigós María Carmen , Jiménez Alfonso
Published: August 2022

This work proposes the revalorization of almond shell (AS) wastes as an active additive for food packaging applications. A new microwave-assisted extraction (MAE) method to obtain extracts rich in polyphenolic compounds with high antioxidant capacity was optimized. An experimental design to optimize the MAE procedure through response surface methodology (RSM) using a Box–Behnken design was proposed. The effects of extraction temperature, irradiation time, ethanol:water concentration, and solvent pH at three levels were evaluated in terms of total phenolic content (TPC) and antioxidant activity (DPPH (2,2-diphenyl-1-picrylhydrazyl) and ferric reducing antioxidant power (FRAP) assays). The optimal conditions found were 57 min, 80 °C, pH 8, and 70% (v/v) ethanol. Optimized MAE extracts showed low soluble protein content (0.43 mg BSA g−1) and were rich in TPC (5.64 mg GAE g−1), flavonoids (1.42 mg CE g−1), and polysaccharides (1.59 mg glucose g−1), with good antioxidant capacity (2.82 mg AAE acid g−1). These results suggest the potential application of these extracts in the food industry as active additives. This strategy opens new pathways to valorize almond shell residues, contributing to the circular economy.
Implementation of a Sponge-Based Flexible Electronic Skin for Safe Human–Robot Interaction
Authors: Yang Kun , Xia Xinkai , Zhang Fan , Ma Huanzhou , Sang Shengbo , Zhang Qiang , Ji Jianlong
Published: August 2022

In current industrial production, robots have increasingly been taking the place of manual workers. With the improvements in production efficiency, accidents that involve operators occur frequently. In this study, a flexible sensor system was designed to promote the security performance of a collaborative robot. The flexible sensors, which was made by adsorbing graphene into a sponge, could accurately convert the pressure on a contact surface into a numerical signal. Ecoflex was selected as the substrate material for our sensing array so as to enable the sensors to better adapt to the sensing application scenario of the robot arm. A 3D printing mold was used to prepare the flexible substrate of the sensors, which made the positioning of each part within the sensors more accurate and ensured the unity of the sensing array. The sensing unit showed a correspondence between the input force and the output resistance that was in the range of 0–5 N. Our stability and reproducibility experiments indicated that the sensors had a good stability. In addition, a tactile acquisition system was designed to sample the tactile data from the sensor array. Our interaction experiment results showed that the proposed electronic skin could provide an efficient approach for secure human–robot interaction.
Investigation of an Electrochromic Device Based on Ammonium Metatungstate-Iron (II) Chloride Electrochromic Liquid
Authors: Kong Sifan , Zhang Guanguang , Li Muyun , Yao Rihui , Guo Chenxiao , Ning Honglong , Zhang Jianzhi , Tao Ruiqiang , Yan Haoyang , Lu Xubing
Published: August 2022

Even though electrochromism has been around for more than 50 years, it still has several issues. Multi-layered films, high manufacturing costs, and a short lifetime are present in existing electrochromic devices. We demonstrate a unique high-performance device with a basic structure and no solid electrochromic sheets in this work. In this device, the electrolyte layer is also avoided. The device uses an electrochromic solution prepared from a mixture of ammonium metatungstate and iron (II) chloride solution as a functional layer with reversible redox properties. The tungstate ions on the electrode surface are reduced when the device is colored, and the Fe2+ on the electrode surface is oxidized on another electrode surface. The generated Fe3+ in the mixed functional layer oxidizes the previously reduced tungstate ions as the device fades. We determined the ΔT (transmittance modulation) and response time among ammonium metatungstate ratios, iron (II) chloride ratios, and driven current density using DOE (design of experiment) trials. Using 0.175 mol/L ammonium metatungstate and 0.30 mol/L iron (II) chloride, a device with outstanding ΔT (more than 57% at 700 nm), a short response time (less than 10 s), and high coloring efficiency (160.04 cm2/C at 700 nm) is demonstrated.
3200 ppi Matrix-Addressable Blue MicroLED Display
Authors: Wu Meng-Chyi , Chung Ming-Che , Wu Cheng-Yeu
Published: August 2022

In this article, an active matrix (AM) micro light-emitting diode (MicroLED) display with a resolution of 1920 × 1080 and a high pixel density of 3200 pixels per inch (ppi) is reported. The single pixel with a diameter of 5 μm on the MicroLED array exhibits excellent characteristics, including a forward voltage of 2.8 V at 4.4 μA, an ideality factor of 1.7 in the forward bias of 2–3 V, an extremely low leakage current of 131 fA at −10 V, an external quantum efficiency of 6.5%, and a wall-plug efficiency of 6.6% at 10.2 A/cm2, a light output power of 28.3 μW and brightness of 1.6 × 105 cd/m2 (nits) at 1 mA. The observed blue shift in the electroluminent peak wavelength is only 6.6 nm from 441.2 nm to 434.6 nm with increasing the current from 5 μA to 1 mA (from 10 to 5 × 103 A/cm2). Through flip-chip bonding technology, the 1920 × 1080 bottom-emitting MicroLED display through the backside of a sapphire substrate can demonstrate high-resolution graphic images.
Multilayer Soft Photolithography Fabrication of Microfluidic Devices Using a Custom-Built Wafer-Scale PDMS Slab Aligner and Cost-Efficient Equipment
Authors: Nguyen Trieu , Sarkar Tanoy , Tran Tuan , Moinuddin Sakib M. , Saha Dipongkor , Ahsan Fakhrul
Published: August 2022

We present a robust, low-cost fabrication method for implementation in multilayer soft photolithography to create a PDMS microfluidic chip with features possessing multiple height levels. This fabrication method requires neither a cleanroom facility nor an expensive UV exposure machine. The central part of the method stays on the alignment of numerous PDMS slabs on a wafer-scale instead of applying an alignment for a photomask positioned right above a prior exposure layer using a sophisticated mask aligner. We used a manual XYZR stage attached to a vacuum tweezer to manipulate the top PDMS slab. The bottom PDMS slab sat on a rotational stage to conveniently align with the top part. The movement of the two slabs was observed by a monocular scope with a coaxial light source. As an illustration of the potential of this system for fast and low-cost multilayer microfluidic device production, we demonstrate the microfabrication of a 3D microfluidic chaotic mixer. A discussion on another alternative method for the fabrication of multiple height levels is also presented, namely the micromilling approach.
Acoustic Manipulation of Intraocular Particles
Authors: Leshno Ari , Kenigsberg Avraham , Peleg-Levy Heli , Piperno Silvia , Skaat Alon , Shpaisman Hagay
Published: August 2022

Various conditions cause dispersions of particulate matter to circulate inside the anterior chamber of a human eye. These dispersed particles might reduce visual acuity or promote elevation of intraocular pressure (IOP), causing secondary complications such as particle related glaucoma, which is a major cause of blindness. Medical and surgical treatment options are available to manage these complications, yet preventive measures are not currently available. Conceptually, manipulating these dispersed particles in a way that reduces their negative impact could prevent these complications. However, as the eye is a closed system, manipulating dispersed particles in it is challenging. Standing acoustic waves have been previously shown to be a versatile tool for manipulation of bioparticles from nano-sized extracellular vesicles up to millimeter-sized organisms. Here we introduce for the first time a novel method utilizing standing acoustic waves to noninvasively manipulate intraocular particles inside the anterior chamber. Using a cylindrical acoustic resonator, we show ex vivo manipulation of pigmentary particles inside porcine eyes. We study the effect of wave intensity over time and rule out temperature changes that could damage tissues. Optical coherence tomography and histologic evaluations show no signs of damage or any other side effect that could be attributed to acoustic manipulation. Finally, we lay out a clear pathway to how this technique can be used as a non-invasive tool for preventing secondary glaucoma. This concept has the potential to control and arrange intraocular particles in specific locations without causing any damage to ocular tissue and allow aqueous humor normal outflow which is crucial for maintaining proper IOP levels.
SARS-CoV-2 Vaccination Coverage and Factors Associated with Low Uptake in a Cohort of People Living with HIV
Authors: Nomah Daniel Kwakye , Llibre Josep Maria , Díaz Yesika , Moreno Sergio , Aceiton Jordi , Bruguera Andreu , Gutiérrez-Macià Maria , Imaz Arkaitz , Suanzes Paula , Navarro Gemma , Orti Amat , Miro Jose Maria , Casabona Jordi , Reyes-Urueña Juliana ,   the PISCIS Study Group
Published: August 2022

People living with HIV (PLWH) are prioritised for SARS-CoV-2 vaccination due to their vulnerability to severe COVID-19. Therefore, the epidemiological surveillance of vaccination coverage and the timely identification of suboptimally vaccinated PLWH is vital. We assessed SARS-CoV-2 vaccination coverage and factors associated with under-vaccination among PLWH in Catalonia, Spain. As of 11.12.2021, 9945/14942 PLWH (66.6%) had received ≥1 dose of a SARS-CoV-2 vaccine. Non-Spanish origin (adjusted odds ratio (aOR) 0.64, 95% CI 0.59–0.70), CD4 count of 200–349 cells/μL (aOR 0.74, 95% CI 0.64–0.86) or 350–499 cells/μL (aOR 0.79, 95% CI 0.70–0.88), detectable plasma HIV-RNA (aOR 0.61 95% CI 0.53–0.70), and previous SARS-CoV-2 diagnosis (aOR 0.58 95% CI 0.51–0.65) were associated with under-vaccination. SARS-CoV-2 diagnosis (437 [9.5%] vs. 323 [3.5%], p < 0.001), associated hospitalisations (10 [2.3%] vs. 0 [0%], p < 0.001), intensive care unit admissions (6 [1.4%] vs. 0 [0%], p < 0.001), and deaths (10 [2.3%] vs. 0 [0%], p < 0.001) were higher among unvaccinated PLWH. Vaccination coverage was lower among PLWH with a CD4 count >200 cells/μL, detectable plasma HIV-RNA, previous SARS-CoV-2 diagnosis, and migrants. SARS-CoV-2 diagnosis, associated hospitalisations, and deaths among PLWH were lower among the vaccinated compared with the unvaccinated. SARS-CoV-2 vaccination prioritisation has not completely reached vulnerable PLWH with poorer prognosis. This information can be used to inform public health strategies.
A Comparison of Rhizospheric and Endophytic Bacteria in Early and Late-Maturing Pumpkin Varieties
Authors: Chen Siyu , Qin Renliu , Yang Da , Liu Wenjun , Yang Shangdong
Published: August 2022

To determine whether rhizospheric and endophytic bacteria contribute to the ripening of pumpkins, an analysis was conducted on rhizospheric and endophytic bacteria and soil fertility in the rhizospheres of early and late-maturing pumpkin varieties. The results showed higher nitrogen and abscisic acid content and more gibberellin-producing bacteria in the rhizospheres or endophytes of the early maturing varieties. Greater soil fertility and more abundant rhizospheric and endophytic bacterial genera with a greater metabolic function might be important mechanisms for early ripening. Rhodococcus, Bacillus, and Arthrobacter can be considered the functional bacteria in promoting pumpkin maturation. On the other hand, Ralstonia could be the functional bacterium that delays ripening.
Fungal Lignocellulose Utilisation Strategies from a Bioenergetic Perspective: Quantification of Related Functional Traits Using Biocalorimetry
Authors: Duong Hieu Linh , Paufler Sven , Harms Hauke , Schlosser Dietmar , Maskow Thomas
Published: August 2022

In the present study, we investigated whether a non-invasive metabolic heat flux analysis could serve the determination of the functional traits in free-living saprotrophic decomposer fungi and aid the prediction of fungal influences on ecosystem processes. For this, seven fungi, including ascomycete, basidiomycete, and zygomycete species, were investigated in a standardised laboratory environment, employing wheat straw as a globally relevant lignocellulosic substrate. Our study demonstrates that biocalorimetry can be employed successfully to determine growth-related fungal activity parameters, such as apparent maximum growth rates (AMGR), cultivation times until the observable onset of fungal growth at AMGR (tAMGR), quotients formed from the AMGR and tAMGR (herein referred to as competitive growth potential, CGP), and heat yield coefficients (YQ/X), the latter indicating the degree of resource investment into fungal biomass versus other functional attributes. These parameters seem suitable to link fungal potentials for biomass production to corresponding ecological strategies employed during resource utilisation, and therefore may be considered as fungal life history traits. A close connection exists between the CGP and YQ/X values, which suggests an interpretation that relates to fungal life history strategies.
Uninvited Guest: Arrival and Dissemination of Omicron Lineage SARS-CoV-2 in St. Petersburg, Russia
Authors: Gladkikh Anna , Dedkov Vladimir , Sharova Alena , Klyuchnikova Ekaterina , Sbarzaglia Valeriya , Arbuzova Tatiana , Forghani Majid , Ramsay Edward , Dolgova Anna , Shabalina Anna , Tsyganova Nadezhda , Totolian Areg
Published: August 2022

Following its emergence at the end of 2021, the Omicron SARS-CoV-2 variant rapidly spread around the world and became a dominant variant of concern (VOC). The appearance of the new strain provoked a new pandemic wave with record incidence rates. Here, we analyze the dissemination dynamics of Omicron strains in Saint Petersburg, Russia’s second largest city. The first case of Omicron lineage BA.1 was registered in St. Petersburg on 10 December 2021. Rapid expansion of the variant and increased incidence followed. The peak incidence was reached in February 2022, followed by an observed decline coinciding with the beginning of spread of the BA.2 variant. SARS-CoV-2 lineage change dynamics were shown in three categories: airport arrivals; clinical outpatients; and clinical inpatients. It is shown that the distribution of lineage BA.1 occurred as a result of multiple imports. Variability within the BA.1 and BA.2 lineages in St. Petersburg was also revealed. On the basis of phylogenetic analysis, an attempt was made to trace the origin of the first imported strain, and an assessment was made of the quarantine measures used to prevent the spread of this kind of infection.
Insights into the Bacterial Diversity and Detection of Opportunistic Pathogens in Mexican Chili Powder
Authors: Hernández Gómez Yoali Fernanda , González Espinosa Jacqueline , Ramos López Miguel Ángel , Arvizu Gómez Jackeline Lizzeta , Saldaña Carlos , Rodríguez Morales José Alberto , García Gutiérrez María Carlota , Pérez Moreno Victor , Álvarez Hidalgo Erika , Nuñez Ramírez Jorge , Jones George H. , Hernández Flores José Luis , Campos Guillén Juan
Published: August 2022

Chili powder is the most frequently consumed spice in Mexican diets. Thus, the dissemination of microorganisms associated with chili powder derived from Capsicum annuum L. is significant during microbial quality analysis, with special attention on detection of potential pathogens. The results presented here describe the initial characterization of bacterial community structure in commercial chili powder samples. Our results demonstrate that, within the domain Bacteria, the most abundant family was Bacillaceae, with a relative abundance of 99% in 71.4% of chili powder samples, while 28.6% of samples showed an average relative abundance of 60% for the Enterobacteriaceae family. Bacterial load for aerobic mesophilic bacteria (AMB) ranged from 104 to 106 cfu/g, while for sporulated mesophilic bacteria (SMB), the count ranged from 102 to 105 cfu/g. Bacillus cereus sensu lato (s.l.) was observed at ca. ˂600 cfu/g, while the count for Enterobacteriaceae ranged from 103 to 106 cfu/g, Escherichia coli and Salmonella were not detected. Fungal and yeast counts ranged from 102 to 105 cfu/g. Further analysis of the opportunistic pathogens isolated, such as B. cereus s.l. and Kosakonia cowanii, using antibiotic-resistance profiles and toxinogenic characteristics, revealed the presence of extended-spectrum β-lactamases (ESBLs) and Metallo-β-lactamases (MBLs) in these organisms. These results extend our knowledge of bacterial diversity and the presence of opportunistic pathogens associated with Mexican chili powder and highlight the potential health risks posed by its use through the spread of antibiotic-resistance and the production of various toxins. Our findings may be useful in developing procedures for microbial control during chili powder production.
Phylogenomic Placement of American Southwest-Associated Clinical and Veterinary Isolates Expands Evidence for Distinct Cryptococcus gattii VGVI
Authors: Monroy-Nieto Juan , Bowers Jolene R. , Montfort Parker , Adame Guillermo , Taverna Constanza Giselle , Yaglom Hayley , Sykes Jane E. , Brady Shane , Mochon A. Brian , Meyer Wieland , Komatsu Kenneth , Engelthaler David M.
Published: August 2022

Whole-genome sequencing has advanced our understanding of the population structure of the pathogenic species complex Cryptococcus gattii, which has allowed for the phylogenomic specification of previously described major molecular type groupings and novel lineages. Recently, isolates collected in Mexico in the 1960s were determined to be genetically distant from other known molecular types and were classified as VGVI. We sequenced four clinical isolates and one veterinary isolate collected in the southwestern United States and Argentina from 2012 to 2021. Phylogenomic analysis groups these genomes with those of the Mexican VGVI isolates, expanding VGVI into a clade and establishing this molecular type as a clinically important population. These findings also potentially expand the known Cryptococcus ecological range with a previously unrecognized endemic area.
Evidence of Community-Wide Spread of Multi-Drug Resistant Escherichia coli in Young Children in Lusaka and Ndola Districts, Zambia
Authors: Bumbangi Flavien Nsoni , Llarena Ann-Katrin , Skjerve Eystein , Hang’ombe Bernard Mudenda , Mpundu Prudence , Mudenda Steward , Mutombo Paulin Beya , Muma John Bwalya
Published: August 2022

Increased antimicrobial resistance (AMR) has been reported for pathogenic and commensal Escherichia coli (E. coli), hampering the treatment, and increasing the burden of infectious diarrhoeal diseases in children in developing countries. This study focused on exploring the occurrence, patterns, and possible drivers of AMR E. coli isolated from children under-five years in Zambia. A hospital-based cross-sectional study was conducted in the Lusaka and Ndola districts. Rectal swabs were collected from 565 and 455 diarrhoeic and healthy children, respectively, from which 1020 E. coli were cultured and subjected to antibiotic susceptibility testing. Nearly all E. coli (96.9%) were resistant to at least one antimicrobial agent tested. Further, 700 isolates were Multi-Drug Resistant, 136 were possibly Extensively-Drug Resistant and nine were Pan-Drug-Resistant. Forty percent of the isolates were imipenem-resistant, mostly from healthy children. A questionnaire survey documented a complex pattern of associations between and within the subgroups of the levels of MDR and socio-demographic characteristics, antibiotic stewardship, and guardians’ knowledge of AMR. This study has revealed the severity of AMR in children and the need for a community-specific-risk-based approach to implementing measures to curb the problem.
S and Sr Isotope Compositions and Trace Element Compositions of the Middle Jurassic Evaporites in Eastern Tibet: Provenance and Palaeogeographic Implications
Authors: Fei Jinna , Shen Lijian , Guan Xin , Sun Zhicheng
Published: August 2022

The origin of Middle Jurassic evaporites in the Qamdo Basin is still controversial because palaeontological studies have reported that they have both marine and continental characteristics. The 87Sr/86Sr ratios of the gypsum in the Middle Jurassic Dongdaqiao Formation in the Qamdo Basin range from 0.707602 to 0.708163, which are higher than that of contemporaneous seawater. Model calculations suggest that continental water prevailed over seawater during the precipitation of these evaporites. However, the majority of the gypsum samples have δ34S values of 15.3‰ to 16.3‰, which are consistent with that of contemporaneous seawater. This range of values (15.3‰ vs. 16.3‰) was likely caused by S isotope fractionation during evaporation because the δ34S values and Sr contents are negatively correlated. The δ34S values of the other three gypsum samples are 20.0‰, 20.5‰, and 20.8‰, which are significantly higher than that of Middle Jurassic seawater. The trace element compositions and scanning electron microscopy (SEM) observations indicate that these elevated δ34S values were caused by bacterial sulphate reduction (BSR). The Sr and S isotope systematics of the gypsums from the Dongdaqiao Formation demonstrate that the parent brines from which the evaporites precipitated were marine based with a large quantity of continental input. A comparison of the lithologies and Sr isotope compositions of the Middle Jurassic sequences in the Qamdo and Qiangtang Basins revealed that the Qiangtang Basin was mainly recharged by Jurassic seawater, while the Qamdo Basin was primarily recharged by continental water with some seawater-derived overflow from the Qiangtang Basin.
Multiphase Diagenetic Processes and Their Impact on Reservoir Character of the Late Triassic (Rhaetian) Kingriali Formation, Upper Indus Basin, Pakistan
Authors: Ahmad Imran , Shah Mumtaz Muhammad , Janjuhah Hammad Tariq , Trave Anna , Antonarakou Assimina , Kontakiotis George
Published: August 2022

Multiple episodes of dolomitization of the shallow marine carbonates of the Late Kingriali Formation resulted in regional scale mappable dolostone geobodies in the Kohat and Potwar sub-basins. With the exception of few unaltered patches of the host limestone, more than 90% of the carbonates of the studied formation are diagenetically altered by replacive dolomites with associated dolomite cementation. Petrographical and geochemical data interpretation reveals that during the initial stage of dolomitization, the precursor limestone was significantly modified by the fabric-retentive replacive dolomite (RD-I) and produced bulk dolostones with non-planar-a to planar-s crystals. Neomorphic recrystallization (RD-II) was observed as an overgrowth of the already formed RD-I dolomite crystals during progressive dolomitization. The seawater at shallow depths is enriched with Fe-ions due to its interaction with Fe-rich beds within the studied formation. The modified seawater actively participated in the formation of ferroan replacive dolomites (RD-III). Stable isotopic composition of the unaltered Echinoderm plates, calcite cement (CC-I), and RD-I demonstrates signatures of δ18O and δ13C within the limit of late Triassic marine seawater or modified seawater. Depletion in the stable oxygen isotopic composition (from −0.99‰ to −3.75‰ V-PDB) demonstrates that RD-II and RD-III were formed in a sequence with progressively higher temperature fluids than normal seawater. Precipitation of dolomite cements as cavity filling rhombs (DC-I) and crystal overgrowth (DC-II) with highly depleted δ18O values (−5.44‰ to −7.45‰ V-PDB) illustrates dolomite cementation at higher temperatures and greater depths. The highly depleted values of δ18O (up to −9.16‰ V-PDB) and (up to 0.42‰ V-PDB) for δ13C of saddle dolomite (SD-I) indicate the precipitation of SD-I as a cavity filling dolomite at considerable depth. Calcite cementation and calcitization actively participated in the early, middle, and late diagenetic modifications as interpreted from their petrographic and stable isotopic studies. Porosity enhancement is clearly demonstrated by dissolution, stylolization, fracturing, and replacement dolomitization. Dolomite and calcite cementation had a negative impact on the reservoir character and occluded the dolostone porosity to a greater extent.
Experimental Study on the Mechanical and Acoustic Emission Characteristics of Tuff with Different Moisture Contents
Authors: Dong Wenlong , Han Lijun , Meng Lingdong , Zhu Hexuan , Yan Shuai , Xu Changyu , Dong Yaning
Published: August 2022

Rock materials are often affected by water in underground engineering. In this study, the mechanical and failure characteristics of tuff under different moisture content were studied using laboratory tests. The moisture content variation of tuff was studied in water absorption tests, and the mechanical and failure characteristics of tuff under different moisture contents were studied through uniaxial compression tests with a Micro-II acoustic emission (AE) control acquisition system. The results showed that the moisture content of tuff increases rapidly at the initial stage of water absorption tests and stabilizes after 180 h of immersion. According to the results of uniaxial compression tests, both uniaxial compressive strength and elasticity modulus decreased with the increase in moisture content. The AE parameters analyses showed that, when the moisture content increased, the accumulated AE counts and energy gradually decreased, and the “quiet period” at the initial stage of uniaxial compression tests lasted longer, and the RA (rise time⁄amplitude)–AF (AE counts⁄duration) distribution and the failure characteristics verified that the failure patterns evolved from shear failure to tensile failure. Scanning electron microscopy was used to observe the morphology of the fracture surface and analyze the influence of moisture content on the fracture characteristics of the tuff at the mesoscopic level. The results of this research can be used as a basis for studying the influence of water on tuff.
Raman Spectroscopy Studies of Equilibrated Ordinary Chondrites with H and L Group and Shock Metamorphism Degrees
Authors: Zhang Yikai , Xia Zhipeng , Miao Bingkui , Huang Jing , Zhang Chuantong , Chen Yan , Chen Guozhu
Published: August 2022

Ordinary chondrites are the most common type of chondrites. As a non-destructive, rapid, and semi-quantitative technology, Raman spectroscopy is widely used in geoscience. This paper presents the results of a Raman spectroscopic study that we conducted for 16 ordinary chondrites with different chemical groups and variable degrees of shock metamorphism. We found that: (1) the relationship between the Fe composition of olivine and pyroxene and the characteristic peaks of the Raman spectrum established by predecessors cannot be refined to the range of meteorites, the shock on meteorites also affects the Raman spectral characteristics of minerals and (2) the full width at half maximum (FWHM) of the shocked minerals (including high-pressure minerals) in meteorites increases in the Raman spectrum, however, no clear numerical relationship with pressure was found. Based on these data, we assess that the feasibility of Raman spectroscopy for the classification of chemical group and shock metamorphism in ordinary chondrites is not well established.
Correction: Wang et al. Exploration of the Inhibitory Potential of Varespladib for Snakebite Envenomation. Molecules 2018, 23, 391
Authors: Wang Yiding , Zhang Jing , Zhang Denghong , Xiao Huixiang , Xiong Shengwei , Huang Chunhong
Published: August 2022

The authors would like to correct an error in the original publication [...]
[1,5]-Hydride Shift Triggered N-Dealkylative Cyclization into 2-Oxo-1,2,3,4-tetrahydroquinoline-3-carboxylates via Boronate Complexes
Authors: Zaitseva Elvira R. , Ivanov Dmitrii S. , Smirnov Alexander Yu. , Mikhaylov Andrey A. , Baleeva Nadezhda S. , Baranov Mikhail S.
Published: August 2022

A new simple one-pot two-step protocol for the synthesis of 2-oxo-1,2,3,4-tetrahydroquinoline-3-carboxylate from 2-(2-(benzylamino)benzylidene)malonate under the action of BF3·Et2O was developed. It was shown that the reaction proceeds through the formation of a stable iminium intermediate containing a difluoroboryl bridge in the dicarbonyl fragment of the molecule.
Analysis of Lipids in Green Coffee by Ultra-Performance Liquid Chromatography–Time-of-Flight Tandem Mass Spectrometry
Authors: Liu Yijun , Chen Min , Li Yimin , Feng Xingqin , Chen Yunlan , Lin Lijing
Published: August 2022

Lipid components in green coffee were clarified to provide essential data support for green coffee processing. The types, components, and relative contents of lipids in green coffee were first analyzed by ultra-performance liquid chromatography–time-of-flight tandem mass spectrometry (UPLC-TOF-MS/MS). The results showed that the main fatty acids in green coffee were linoleic acid (43.39%), palmitic acid (36.57%), oleic acid (8.22%), and stearic acid (7.37%). Proportionally, the ratio of saturated fatty acids/unsaturated fatty acids/polyunsaturated fatty acids was close to 5.5:1:5.2. A total of 214 lipids were identified, including 15 sterols, 39 sphingosines, 12 free fatty acids, 127 glycerides, and 21 phospholipids. The main components of sterols, sphingosines, free fatty acids, glycerides, and phospholipids were acylhexosyl sitosterol, ceramide esterified omega-hydroxy fatty acid sphingosine, linoleic acid, and triglyceride, respectively. UPLC-TOF-MS/MS furnished high-quality and accurate information on TOF MS and TOF MS/MS spectra, providing a reliable analytical technology platform for analyzing lipid components in green coffee.
DES-Based Biocatalysis as a Green Alternative for the l-menthyl Ester Production Based on l-menthol Acylation
Authors: Ion Sabina , Olănescu Florentina , Teodorescu Florina , Tincu Robert , Gheorghe Daniela , Pârvulescu Vasile I. , Tudorache Mădălina
Published: August 2022

The deep eutectic solvent (DES)-based biocatalysis of l-menthol acylation was designed for the production of fatty acid l-menthyl ester (FME) using fatty acid methyl ester (FAME). The biocatalytic reaction was assisted by a lipase enzyme in the DES reaction medium. ւՒ-menthol and fatty acids (e.g., CA—caprylic acid; OA—oleic acid; LiA—linoleic acid; and LnA—linolenic acid) were combined in the binary mixture of DES. In this way, the DES provided a nonpolar environment for requested homogeneity of a biocatalytic system with reduced impact on the environment. The screening of lipase enzyme demonstrated better performance of immobilized lipase compared with powdered lipase. The performance of the biocatalytic system was evaluated for different DES compositions (type and concentration of the acid component). l-menthol:CA = 73:27 molar ratio allowed it to reach a maximum conversion of 95% methyl lauric ester (MLE) using a NV (Candida antarctica lipase B immobilized on acrylic resin) lipase biocatalyst. The recyclability of biocatalysts under optimum conditions of the system was also evaluated (more than 80% recovered biocatalytic activity was achieved for the tested biocatalysts after five reaction cycles). DES mixtures were characterized based on differential scanning calorimetry (DSC) and refractive index analysis.