bluuebunny/crossref_metadata_2025 huggingface dataset:
The Aggravating Role of Failing Neuropeptide Networks in the Development of Sporadic Alzheimer’s Disease
Authors: Jászberényi Miklós , Thurzó Balázs , Jayakumar Arumugam R. , Schally Andrew V.
Published: December 2024

Alzheimer’s disease imposes an increasing burden on aging Western societies. The disorder most frequently appears in its sporadic form, which can be caused by environmental and polygenic factors or monogenic conditions of incomplete penetrance. According to the authors, in the majority of cases, Alzheimer’s disease represents an aggravated form of the natural aging of the central nervous system. It can be characterized by the decreased elimination of amyloid β1–42 and the concomitant accumulation of degradation-resistant amyloid plaques. In the present paper, the dysfunction of neuropeptide regulators, which contributes to the pathophysiologic acceleration of senile dementia, is reviewed. However, in the present review, exclusively those neuropeptides or neuropeptide families are scrutinized, and the authors’ investigations into their physiologic and pathophysiologic activities have made significant contributions to the literature. Therefore, the pathophysiologic role of orexins, neuromedins, RFamides, corticotrope-releasing hormone family, growth hormone-releasing hormone, gonadotropin-releasing hormone, ghrelin, apelin, and natriuretic peptides are discussed in detail. Finally, the therapeutic potential of neuropeptide antagonists and agonists in the inhibition of disease progression is discussed here.
The microRNA-29ab1/Zfp36/AR Axis in the Hypothalamus Regulates Male-Typical Behaviors in Mice
Authors: Ma Jie , Lin Yingying , Xiong Wei , Liu Xiaoxue , Pan Minghui , Sun Jiazeng , Sun Yanan , Li Yixuan , Guo Huiyuan , Pang Guofang , Wang Xiaoyu , Ren Fazheng
Published: December 2024

Male-typical behaviors such as aggression and mating, which reflect sexual libido in male mice, are regulated by the hypothalamus, a crucial part of the nervous system. Previous studies have demonstrated that microRNAs (miRNAs), especially miR-29, play a vital role in reproduction and the neural control of behaviors. However, it remains unclear whether miR-29 affects reproduction through the hypothalamus-mediated regulation of male-typical behaviors. Here, we constructed two mouse knockout models by ablating either the miR-29ab1 or miR-29b2c cluster. Compared to WT, the ablation of miR-29ab1 in male mice significantly reduced the incidence of aggression by 60% and the incidence of mating by 46.15%. Furthermore, the loss of miR-29ab1 in male mice led to the downregulation of androgen receptor (AR) in the ventromedial hypothalamus. Transcriptomic analysis of the hypothalamus of miR-29ab1-deficient mice revealed inflammatory activation and aberrant expression of genes associated with male-typical behaviors, including Ar, Pgr, Htr4, and Htr2c. Using bioinformatics analysis and dual-luciferase reporter assays, we identified zinc finger protein 36 (Zfp36) as a direct downstream target gene of miR-29ab1. We subsequently showed that ZFP36 colocalized with AR in GT1-7 cells. Furthermore, inhibition of Zfp36 or RelB in GT1-7 cells led to an increase in AR expression. Collectively, our results demonstrate that the miR-29ab1/Zfp36/AR axis in the hypothalamus plays a pivotal role in the regulation of aggression and mating in male mice, providing a potential therapeutic target for treating infertility caused by low libido.
Bioengineered Extracellular Vesicle Hydrogel Modulating Inflammatory Microenvironment for Wound Management
Authors: Mu Yunfei , Ma Liwen , Yao Jia , Luo Dan , Ding Xianguang
Published: December 2024

Chronic wounds, frequently arising from conditions like diabetes, trauma, or chronic inflammation, represent a significant medical challenge due to persistent inflammation, heightened infection risk, and limited treatment solutions. This study presents a novel bioengineered approach to promote tissue repair and improve the healing environment. We developed a bioactive hydrogel patch, encapsulated zeolitic imidazolate framework-8 (ZIF-8) into extracellular vesicles (EVs) derived from anti-inflammatory M2 macrophages, and synthesized ZIF@EV, then embedded it in the sodium alginate matrix. This hydrogel structure enables the controlled release of therapeutic agents directly into the wound site, where it stimulates endothelial cell proliferation and promotes new blood vessel formation. These processes are key components of effective tissue regeneration. Crucially, the EV-infused patch influences the immune response by polarizing macrophages towards an M2 phenotype, shifting the wound environment from inflammation toward regenerative healing. When applied in a murine model of chronic wounds, the EV hydrogel patch demonstrated notable improvements in healing speed, quality, and tissue integration compared to traditional approaches such as growth factor therapies and foam dressings. These promising findings suggest that this bioactive hydrogel patch could serve as a versatile, practical solution for chronic wound management, providing an adaptable platform that addresses both the biological and logistical needs of wound care in clinical settings.
Understanding Vascular Calcification in Chronic Kidney Disease: Pathogenesis and Therapeutic Implications
Authors: Siracusa Chiara , Carabetta Nicole , Morano Maria Benedetta , Manica Marzia , Strangio Antonio , Sabatino Jolanda , Leo Isabella , Castagna Alberto , Cianflone Eleonora , Torella Daniele , Andreucci Michele , Zicarelli Maria Teresa , Musolino Michela , Bolignano Davide , Coppolino Giuseppe , De Rosa Salvatore
Published: December 2024

Vascular calcification (VC) is a biological phenomenon characterized by an accumulation of calcium and phosphate deposits within the walls of blood vessels causing the loss of elasticity of the arterial walls. VC plays a crucial role in the incidence and progression of chronic kidney disease (CKD), leading to a significant increase in cardiovascular mortality in these patients. Different conditions such as age, sex, dyslipidemia, diabetes, and hypertension are the main risk factors in patients affected by chronic kidney disease. However, VC may occur earlier and faster in these patients if it is associated with new or non-traditional risk factors such as oxidative stress, anemia, and inflammation. In chronic kidney disease, several pathophysiological processes contribute to vascular calcifications, including osteochondrogenic differentiation of vascular cells, hyperphosphatemia and hypercalcemia, and the loss of specific vascular calcification inhibitors including pyrophosphate, fetuin-A, osteoprotegerin, and matrix GLA protein. In this review we discuss the main traditional and non-traditional risk factors that can promote VC in patients with kidney disease. In addition, we provide an overview of the main pathogenetic mechanisms responsible for VC that may be crucial to identify new prevention strategies and possible new therapeutic approaches to reduce cardiovascular risk in patients with kidney disease.
Identification of MAPK Genes in Phaseolus vulgaris and Analysis of Their Expression Patterns in Response to Anthracnose
Authors: Liu Huiling , Wang Da , Wang Zhenyu , Zhao Tong , Zhang Jingying , Wang Yan , Qiao Hongyu , Han Yuzhu
Published: December 2024

The oil bean is a high-quality, economically valuable variety of kidney bean (Phaseolus vulgaris L.) that is widely cultivated in Northeast China. However, the prevalence of anthracnose, caused by a combination of factors, including continuous cropping over many years, has led to significant declines in both yield and quality. The mitogen-activated protein kinase (MAPK) cascade is a highly conserved plant cell signaling pathway that plays a pivotal role in plant growth and development, as well as responses to biotic stress. However, its role in the response of P. vulgaris to anthracnose infection has not previously been reported. We identified and characterized thirteen MAPK genes (PvMAPK01–PvMAPK13) in the P. vulgaris genome. These genes were found on eight of the eleven chromosomes of P. vulgaris, and phylogenetic analyses classified them into four previously established subgroups (A–D). Analysis of the cis-acting elements in their promoter regions revealed the presence of multiple elements associated with light, hormone regulation, stress responses, and growth and development. An analysis of intraspecific collinearity revealed that whole-genome and/or segmental duplication, rather than tandem duplication, has been the primary driver of PvMAPK family expansion in P. vulgaris. Transcriptome data revealed that the PvMAPKs differed in their tissue-specific expression patterns, with PvMAPK05 showing particularly high expression in stems and stem tips and PvMAPK07 and PvMAPK11 showing relatively low expression across all tissues. In general, expression of the PvMAPKs was higher in stems, stem tips, and pods than in other tissues and organs, suggesting that they may be particularly important for regulating stem and pod development. Analysis of the expression of PvMAPKs in field-grown plants infected or uninfected with anthracnose revealed that the relative expression levels of PvMAPK05, PvMAPK07, PvMAPK09, and PvMAPK11 exhibited particularly significant changes in response to anthracnose infection across different varieties, suggesting their potential involvement in the anthracnose response of Phaseolus vulgaris. This study reports the fundamental characteristics of the thirteen MAPK genes in P. vulgaris, documents their expression patterns in diverse tissues, and offers preliminary insights into their responses to anthracnose infection, establishing a foundation for subsequent functional validation.
Machine Learning and Metabolomics Predict Mesenchymal Stem Cell Osteogenic Differentiation in 2D and 3D Cultures
Authors: Klontzas Michail E. , Vernardis Spyros I. , Batsali Aristea , Papadogiannis Fotios , Panoskaltsis Nicki , Mantalaris Athanasios
Published: December 2024

Stem cells have been widely used to produce artificial bone grafts. Nonetheless, the variability in the degree of stem cell differentiation is an inherent drawback of artificial graft development and requires robust evaluation tools that can certify the quality of stem cell-based products and avoid source-tissue-related and patient-specific variability in outcomes. Omics analyses have been utilised for the evaluation of stem cell attributes in all stages of stem cell biomanufacturing. Herein, metabolomics in combination with machine learning was utilised for the benchmarking of osteogenic differentiation quality in 2D and 3D cultures. Metabolomics analysis was performed with the use of gas chromatography–mass spectrometry (GC-MS). A set of 11 metabolites was used to train an XGboost model which achieved excellent performance in distinguishing between differentiated and undifferentiated umbilical cord blood mesenchymal stem cells (UCB MSCs). The model was benchmarked against samples not present in the training set, being able to efficiently capture osteogenesis in 3D UCB MSC cultures with an area under the curve (AUC) of 82.6%. On the contrary, the model did not capture any differentiation in Wharton’s Jelly MSC samples, which are well-known underperformers in osteogenic differentiation (AUC of 56.2%). Mineralisation was significantly correlated with the levels of fumarate, glycerol, and myo-inositol, the four metabolites found most important for model performance (R2 = 0.89, R2 = 0.94, and R2 = 0.96, and p = 0.016, p = 0.0059, and p = 0.0022, respectively). In conclusion, our results indicate that metabolomics in combination with machine learning can be used for the development of reliable potency assays for the evaluation of Advanced Therapy Medicinal Products.
Prognostic Assessment with the Malnutrition Universal Screening Tool in Heart Transplant Recipients: A Pilot Study and a Single-Center Experience
Authors: Fabozzo Assunta , Lombardi Valentina , Cibin Giorgia , Bergonzoni Emma , Lorenzoni Giulia , Gregori Dario , Tessari Chiara , Bacich Daniela , D’Onofrio Augusto , Toscano Giuseppe , Gambino Antonio , Tarzia Vincenzo , Pradegan Nicola , Gerosa Gino
Published: December 2024

Introduction and aims: Malnutrition is associated with increased morbidity and mortality in patients who undergo cardiac surgery. Nevertheless, objective assessment of malnourished patients undergoing heart transplantation (HT) is limited. We aimed to analyze the relationship between the malnutrition status and the early and late clinical outcomes of patients undergoing HT using a novel semi-quantitative tool. Methods: All patients aged ≥18 years who underwent HT between January 2015 and July 2020 in a single center were retrospectively evaluated and included in the study. The semi-quantitative Malnutrition Universal Screening Tool (MUST) score (already validated in heart failure) was calculated for each patient at the time of transplantation to assess their nutritional status. A propensity score weighting approach was performed to evaluate the association between the increase in MUST score and the risk of early complications and in-hospital mortality. A Cox regression analysis was performed to assess follow-up mortality. Results: A total of 168 HT patients (median age 58.4 years, IQR 49.5–65.2, men n = 128, 76%) were included within the study period. Their median preoperative BMI was 24.0 kg/m2 (IQR 21.2–27.9). Preoperative MUST scores of 0, 1, and ≥2 were found in 92 (55%), 24 (14%), and 52 (31%) patients, respectively. The median preoperative eGFR was 64.3 mL/min (IQR 49.0–83.2). An increase in MUST score (from 0 to 2) was not significantly related to major postoperative complications or in-hospital mortality. An analogous increase in MUST score was associated with increased follow-up mortality risk (hazard ratio 1.28, 95% CI 1.04–1.83, p = 0.024). Conclusions: Malnutrition assessed with the MUST score seems not to be associated with increased in-hospital mortality or major postoperative complications in patients who undergo HT, but according to our preliminary data it is related to patients’ long-term mortality.
Personalized Antifibrotic Therapy in CKD Progression
Authors: Delrue Charlotte , Eisenga Michele F. , Delanghe Joris R. , Speeckaert Marijn M.
Published: December 2024

Chronic kidney disease (CKD) is a chronic disorder characterized by kidney fibrosis and extracellular matrix accumulation that can lead to end-stage kidney disease. Epithelial-to-mesenchymal transition, inflammatory cytokines, the TGF-β pathway, Wnt/β-catenin signaling, the Notch pathway, and the NF-κB pathway all play crucial roles in the progression of fibrosis. Current medications, such as renin–angiotensin–aldosterone system inhibitors, try to delay disease development but do not stop or reverse fibrosis. This review emphasizes the growing need for tailored antifibrotic medications for CKD treatment. Precision medicine, which combines proteomic, metabolomic, and genetic data, provides a practical way to personalize treatment regimens. Proteomic signatures, such as CKD273, and genetic markers, such as APOL1 and COL4A5, help in patient stratification and focused therapy development. Two recently developed antifibrotic medications, nintedanib and pirfenidone, have been proven to diminish fibrosis in preclinical animals. Additionally, research is being conducted on the efficacy of investigational drugs targeting CTGF and galectin-3 in the treatment of kidney fibrosis.
Effects of Promotional Bundles with Non-Fungible Token (NFT) Fashion on Consumers’ Perceptions
Authors: Kim Seong Eun , Lee Jung Eun , Youn Song-yi
Published: December 2024

The rapid expansion of the non-fungible token (NFT) market, which grew over 200% in 2023 to reach $22 billion, has opened new avenues for fashion brands to engage consumers through digital fashion products under blockchain technology. This study investigated the effects of NFT promotional bundles that combine physical and NFT fashion items as a pair on consumer perceptions. By investigating the interaction effect between the brand type (luxury vs. non-luxury) and promotional bundle types (PHY+free NFT vs. NFT+free PHY), the research demonstrated how these bundles influenced consumers’ perceived value, risk, and authenticity according to the brand type. The findings showed that while a freebie physical item can enhance consumers’ perceived value of NFT products for non-luxury brands, it led to value-discounting inferences, particularly for luxury brands. This study contributes to the literature on NFT fashion by exploring consumer perceptions and providing insights for fashion retailers on effectively framing promotional bundles to maximize consumer engagement for NFT fashion products.
Observation of Boson Peak of Fragile Baltic Amber Glass by Terahertz Time-Domain Spectroscopy
Authors: Sasaki Toru , Oh Soo Han , Mori Tatsuya , Kojima Seiji
Published: December 2024

Amber is a fragile (in Angell’s classification) natural glass that has performed maturation processes over geological time. The terahertz dynamics of Baltic amber that was about 40 million years old were studied by terahertz time-domain spectroscopy (THz-TDS) in the frequency range of 0.2 and 6.0 THz. In general, the intensity of a boson peak is weak for fragile glass. In the terahertz transmission spectra of Baltic amber in the previous study, no boson peak was observed upon slow cooling. However, in the present study, upon rapid cooling down to 15 K, the boson peak was observed clearly at 0.36 THz by the suppression of ice nucleation of confined water. The dynamic correlation length determined by the boson peak frequency was compared with the static structure correlation length and the scale of the medium-range order as determined by the first sharp diffraction peak of X-ray diffraction (XRD) in the recent literature. It was found that the dynamic correlation length determined by THz-TDS was closely related to the static correlation length determined by the XRD analysis.
Investigation on a Novel Reinforcement Method of Grouting Sleeve Connection Considering the Absence of Reserved Reinforcing Bars in the Transition Layer
Authors: Gu Sheng , Yang Jun , Shen Saifeng , Li Xing
Published: December 2024

In practical engineering, due to quality inspections of connections between prefabricated components and construction errors, reserved reinforcing bars in the transition layer may be partially insufficient or even completely absent. This defect significantly impacts the structural performance of sleeve connections, particularly under tensile or shear forces. This paper proposes a novel reinforcement method to address the connection issues caused by the absence of reserved reinforcing bars in the transition layer and verifies its feasibility through systematic experiments. To this end, this paper proposed a novel reinforcement method of grouting sleeve connection considering the absence of reserved bars in the transition layer, and 45 specimens with different reinforcement parameters were fabricated and tested under tension. Before verifying the reliability of the novel reinforcement method, nine specimens were fabricated and tested to verify the weldability of grouting sleeves and reinforcing bars. According to the test results, the fully grouted sleeves, including Grade 45 steel and Q345, showed good weldability with the HRB400 steel bars, while the ductile iron grouted sleeve showed poor weldability. When the single-sided welding length was greater than or equal to six times the diameter of the post-retrofitted connecting steel bar (D2), the primary failure mode observed in specimens utilizing the novel reinforcement method was the fracture of the prefabricated steel bar. The novel reinforcement method could be used to repair the defect of the grouting sleeve connection considering the absence of reserved reinforcing bars in the transition layer. When the single-sided welding length was 4D2, with a relative protective layer thickness of 2D2, and using C60 grade reinforcement material, this combination of conditions represented the critical condition to avoid weld failure between the grouting sleeve and the post-retrofitted connecting steel bars. In practical reinforcement projects, it is suggested that the single-sided welding length should be 5D2, the relative protective layer thickness should be 3D2, and the reinforcement material strength should be C60.
Our Experience and Clinical Findings in Perineal Burns: Implications for Patient Prognosis—A 3 Year Retrospective Study
Authors: Iordache Matei , Bordeanu-Diaconescu Eliza-Maria , Grosu-Bularda Andreea , Andrei Mihaela-Cristina , Frunza Adrian , Grama Sabina , Costache Raducu , Neagu Tiberiu-Paul , Lascar Ioan , Hariga Cristian-Sorin
Published: December 2024

Background and Objectives: Burn injury represents a very important public health problem that affects all age groups. Of all burns, of particular interest is that of the perineum. Despite the importance of the subject, unfortunately, the medical literature on this anatomical region is sparse. With this study we aim to analyze the characteristics of burns affecting the perineal area, the physiopathologic implications of this injury, the influence of patient prognosis, possible complications and therapeutic guidelines. Materials and Methods: This study is formed by a retrospective analysis of cases that were admitted over a period spanning 3 years with a total of 258 burned patients. After inclusion criteria, we selected 49 patients who had perineal burns and compared this group to a non-perineal burns lot of 198 patients (11 were excluded). We studied their characteristics and the demographical aspects that we deemed most important to their condition: age, sex, burn percentage of total body surface area (TBSA), the percentage of third-degree lesions, comorbidities, and associated infections, inhalation injuries and we calculated the significant scores such as the Abbreviated Burn Severity Index score (ABSI). Results: The patients in our study mostly had severe extensive burns (64.9% mean TBSA) which were also underlined by the mean ABSI of 10.88 ± 2.46 thus having a poor prognosis considering their age, the percentage of burned area, the presence of third-degree burns and inhalation injuries. In our study, perineal burns were usually associated with burns of adjacent regions abdominal wall burns comprising 51% and thigh burns comprising 97.9% of the associated injuries. This relationship both explains their presence in mostly severe cases with higher TBSA and also underlines the issues that derive from the burns of the perineum and their several complications which lead to an unbalance of the patients. The treatment of perineal burns still remains much debated in the literature when considering their indications and can become rather complex in the sequelae setting. Conclusions: The issue of burns remains one of the most important subjects in plastic surgery. Being a region hard to treat but with a big influence on patient evolution and survival chances prevention remains a key factor.
Talent Management Practices and Employee Retention: Does It Make a Difference?
Authors: Almashyakhi Ali Mohammed
Published: December 2024

The role of talent management is to ensure that employees’ special skills and talents are identified, and that the right jobs are assigned along their career paths. This study examines the relationship between talent management and employee retention within the Saudi Arabia government sector, specifically among civil service employees working for the Saudi government. A sample size of 440 participants was used in the study. The results revealed that talent management practices are positively related to employee retention. These findings highlight the importance for organizations, especially in the public sector, to implement effective talent retention strategies to retain their most skilled employees over the long term. This research contributes to closing the existing gap in understanding talent management and retention within the context of Saudi Arabia’s civil service sector.
The ARK2N (C18ORF25) Genetic Variant Is Associated with Muscle Fiber Size and Strength Athlete Status
Authors: Çığırtaş Rukiye , Bulgay Celal , Kazan Hasan Hüseyin , Akman Onur , Sporiš Goran , John George , Yusupov Rinat A. , Sultanov Rinat I. , Zhelankin Andrey V. , Semenova Ekaterina A. , Larin Andrey K. , Kulemin Nikolay A. , Generozov Edward V. , Jurko Damir , Ahmetov Ildus I.
Published: December 2024

Background: Data on the genetic factors contributing to inter-individual variability in muscle fiber size are limited. Recent research has demonstrated that mice lacking the Arkadia (RNF111) N-terminal-like PKA signaling regulator 2N (Ark2n; also known as C18orf25) gene exhibit reduced muscle fiber size, contraction force, and exercise capacity, along with defects in calcium handling within fast-twitch muscle fibers. However, the role of the ARK2N gene in human muscle physiology, and particularly in athletic populations, remains poorly understood. The aim of this study was threefold: (a) to compare ARK2N gene expression between power and endurance athletes; (b) to analyze the relationship between ARK2N gene expression and muscle fiber composition; and (c) to investigate the association between the functional variant of the ARK2N gene, muscle fiber size, and sport-related phenotypes. Results: We found that ARK2N gene expression was significantly higher in power athletes compared to endurance athletes (p = 0.042) and was positively associated with the proportion of oxidative fast-twitch (type IIA) muscle fibers in untrained subjects (p = 0.017, adjusted for age and sex). Additionally, we observed that the ARK2N rs6507691 T allele, which predicts high ARK2N gene expression (p = 3.8 × 10−12), was associated with a greater cross-sectional area of fast-twitch muscle fibers in strength athletes (p = 0.015) and was over-represented in world-class strength athletes (38.6%; OR = 2.2, p = 0.023) and wrestlers (33.8%; OR = 1.8, p = 0.044) compared to controls (22.0%). Conclusions: In conclusion, ARK2N appears to be a gene specific to oxidative fast-twitch myofibers, with its functional variant being associated with muscle fiber size and strength-athlete status.
Designing Microfluidic-Chip Filtration with Multiple Channel Networks for the Highly Efficient Sorting of Cell Particles
Authors: Chun Myung-Suk
Published: December 2024

Microfluidic-chip based hydrodynamic filtration is one of the passive sorting techniques that can separate cell or particle suspensions into subpopulations of different sizes. As the branch channels and side channels play an important role in maintaining particle focusing, their rational design is necessary for highly efficient sorting. A model framework involving multiple side and multiple branch channels has been developed by extending the analytical analysis of three-dimensional laminar flow in channel networks, which was previously validated by comparison with numerical simulations. Objective parameters were identified as the number of branch channels and each length of individual branches. The presence of multiple side channels causes an increase in the average fluid velocity in main and branch channels as the branch point shifts toward the end of the main channel, which differs from the behavior observed in a single side channel. The number of branches and their individual lengths decrease distinctly in the case of branch channels consisting of narrow and wide sections, which enables the compact design of a microfluidic-chip, being operated by a lower pressure drop under the same throughput. Sorting of bidisperse particles was accomplished with an optimally designed chip to verify this framework by achieving very high recovery and purity.
Controlled Hydrogen Loading of Magnesium Thin Films in KOH—Effects on the Hydride Nucleation and Growth Regimes
Authors: Guardi Giorgia , Sarapulova Angelina , Dsoke Sonia , Wagner Stefan , Pasquini Luca , Pundt Astrid
Published: December 2024

In this work, we propose a strategy to optimize electrochemical hydrogen loading in magnesium–palladium thin films, using 5 M KOH as an electrolyte. Mg thin films of thickness 26 nm were deposited on sapphire (0001) substrates and capped by a 32 nm Pd layer. By performing cyclic voltammetry with in situ optical microscopy, it appears that a loading potential of at least −1.2 V vs. Hg/HgO has to be achieved at the sample’s surface to trigger magnesium hydride formation. Loading potential effects are then further explored by hydrogenography, where different hydride formation mechanisms appear based on the actual potential. With a larger loading potential of −1.6 V vs. Hg/HgO, a magnesium hydride blocking layer is formed; in this case, Pd hydride temporarily forms in the capping layer as hydrogen diffuses towards the magnesium layer. Loading is optimized for a lower potential of −1.2 V vs. Hg/HgO, which leads to larger hydride precipitates and delays the blocking layer formation; in this case, Pd hydride only appears after the magnesium layer is completely hydrided.
Quercetin and Mesenchymal Stem Cell Metabolism: A Comparative Analysis of Young and Senescent States
Authors: Ivan Alexandra , Lukinich-Gruia Alexandra Teodora , Cristea Iustina-Mirabela , Pricop Maria-Alexandra , Calma Crenguta Livia , Simina Alina-Georgiana , Tatu Călin Adrian , Galuscan Atena , Păunescu Virgil
Published: December 2024

Quercetin is a natural flavonoid renowned for its potent antioxidant, anti-inflammatory, anti-diabetic, and antibacterial properties, making it a highly promising candidate for the treatment of various medical conditions. Our current study investigates the influence of quercetin on energy metabolism, fatty acid composition, oxidative stress gene expression, and sirtuin expression in early- and late-stage passages of stem cells derived from human exfoliated deciduous teeth (SHEDs). Mitochondrial respiration was analyzed by measuring oxygen consumption following a 24 h quercetin treatment, while fatty acid profiles were examined using gas chromatography–mass spectrometry (GC-MS). Additionally, quantitative PCR (qPCR) was used to assess the expression of oxidative stress genes and sirtuins. In younger SHEDs, quercetin enhances metabolic activity and mitochondrial respiration, although higher doses may decrease mitochondrial activity. Conversely, in older, senescent SHEDs, quercetin supports mitochondrial function at lower concentrations but appears to inhibit respiration at higher doses. These results suggest that quercetin may hold therapeutic potential for maintaining SHED viability and function, especially at lower doses in older cells. Further research is essential to fully elucidate a dose-dependent effect of quercetin and optimize its applications in regenerative medicine.
Effects of Substrate Temperature on Optical, Structural, and Surface Properties of Metal–Organic Vapor Phase Epitaxy-Grown MgZnO Films
Authors: Liu Jiamin , Xie Deng , Feng Zhe Chuan , Nafisa Manika Tun , Wan Lingyu , Qiu Zhi-Ren , Wuu Dong-Sing , Zhang Chuanwei , Yiin Jeffrey , Lin Hao-Hsiung , Lu Weijie , Klein Benjamin , Ferguson Ian T. , Liu Shiyuan
Published: December 2024

MgZnO possesses a tunable bandgap and can be prepared at relatively low temperatures, making it suitable for developing optoelectronic devices. MgxZn1−xO (x~0.1) films were grown on sapphire by metal–organic vapor phase epitaxy under different substrate-growth temperatures Ts of 350–650 °C and studied by multiple characterization technologies like X-ray diffraction (XRD), spectroscopic ellipsometry (SE), Raman scattering, extended X-ray absorption fine structure (EXAFS), and first-principle calculations. The effects of Ts on the optical, structural, and surface properties of the Mg0.1Zn0.9O films were studied penetratively. An XRD peak of nearly 35° was produced from Mg0.1Zn0.9O (0002) diffraction, while a weak peak of ~36.5° indicated MgO phase separation. SE measurements and analysis determined the energy bandgaps in the 3.29–3.91 eV range, obeying a monotonically decreasing law with increasing Ts. The theoretical bandgap of 3.347 eV, consistent with the SE-reported value, demonstrated the reliability of the SE measurement. Temperature-dependent UV-excitation Raman scattering revealed 1LO phonon splitting and temperature dependency. Zn-O and Zn-Zn atomic bonding lengths were deduced from EXAFS. It was revealed that the surface Mg amount increased with the increase in Ts. These comprehensive studies provide valuable references for Mg0.1Zn0.9O and other advanced materials.
Mechanisms of Neurosyphilis-Induced Dementia: Insights into Pathophysiology
Authors: Fadel Aya , Hussain Hussain , Hernandez Robert J. , Clichy Silva Amanda Marie , Estil-las Amir Agustin , Hamad Mohammad , Saadoon Zahraa F. , Naseer Lamia , Sultan William C. , Sultan Carla , Schnepp Taylor , Jayakumar Arumugam R.
Published: December 2024

Neurosyphilis-induced dementia represents a severe manifestation of tertiary syphilis, characterized by cognitive and neuropsychiatric impairments. This condition arises from the progression of syphilis to the central nervous system, where the spirochete causes damage through invasion, chronic inflammation, and neurodegeneration. The pathophysiology involves chronic inflammatory responses, direct bacterial damage, and proteinopathies. Treponema pallidum triggers an inflammatory cascade, resulting in neuronal injury and synaptic dysfunction. Abnormal protein accumulations, including TAR DNA-binding protein 43 (TDP-43) and tau, contribute to neuronal loss and cognitive decline. Seizures, psychiatric symptoms, and motor deficits further complicate the progression of dementia. Diagnosis includes clinical assessment, cerebrospinal fluid analysis, and neuroimaging. Diagnostic tests include CSF-VDRL, FTA-ABS, and neuroimaging techniques such as MRI and PET scans, which help detect structural changes and confirm neurosyphilis. Management of neurosyphilis-induced dementia involves antibiotic therapy and psychotropic medications to address both infectious and symptomatic components. While penicillin remains the cornerstone of treatment, psychotropic agents, including haloperidol, risperidone, quetiapine, and divalproex sodium, can manage psychiatric symptoms. However, careful monitoring is required due to potential side effects and interactions with ongoing treatment. Overall, early diagnosis and comprehensive management are crucial for mitigating the cognitive and neuropsychiatric impairments associated with neurosyphilis-induced dementia.
Bioactivated Glucoraphanin Improves Cell Survival, Upregulating Phospho-AKT, and Modulates Genes Involved in DNA Repair in an In Vitro Alzheimer’s Disease Model: A Network-Transcriptomic Analysis
Authors: Minuti Aurelio , Mazzon Emanuela , Iori Renato , Chiricosta Luigi , Artimagnella Osvaldo
Published: December 2024

Background/Objectives: Alzheimer’s disease (AD) is one of the most common neurodegenerative diseases, for which a definitive cure is still missing. Recently, natural compounds have been investigated for their possible neuroprotective role, including the bioactivated product of glucoraphanin (GRA), the sulforaphane (SFN), which is highly rich in cruciferous vegetables. It is known that SFN alleviates neuronal dysfunction, apoptosis, and oxidative stress in the brain. In the light of this evidence, the aim of this study was to investigate the molecular effects of SFN pre-treatment in differentiated SH-SY5Y neurons exposed to β-amyloid (Aβ). Methods: To this end, we first evaluated first cell viability via the Thiazolyl Blue Tetrazolium Bromide (MTT) assay, and then we analyzed the transcriptomic profiles by next-generation sequencing (NGS). Finally, we used a network analysis in order to understand which biological processes are affected, validating them by Western blot assay. Results: SFN pre-treatment counteracted Aβ-induced loss of cell viability. The network-transcriptomic analysis revealed that SFN upregulates genes associated with DNA repair, such as ABRAXAS1, BRCA1, BRCA2, CDKN1A, FANCA, FANCD2, FANCE, NBN, and XPC. Finally, SFN also increased the phosphorylation of AKT, which is associated with DNA repair and cell survival. Conclusions: These data suggest that SFN is a natural compound that could be suitable in the prevention of AD, thanks to its neuroprotective role in increasing cell survival, potentially restoring DNA damage induced by Aβ exposure.
The Cardioprotective Effects of Nutritional Ketosis: Mechanisms and Clinical Implications
Authors: Venturini Claudia , Mancinelli Lucia , Matacchione Giulia , Olivieri Fabiola , Antonicelli Roberto
Published: December 2024

Cardiovascular diseases (CVDs) persist as the primary cause of death worldwide, accounting for roughly 17.9 million fatalities each year. The prevalence of obesity, metabolic syndrome, and type 2 diabetes (key risk factors for CVD) continues to escalate at an alarming rate, necessitating novel therapeutic strategies to address this global health crisis. Nutritional ketosis, induced through ketogenic diets, modified fasting, intermittent fasting, and medium-chain triglyceride (MCT) oil consumption, has garnered attention for its potential cardioprotective effects. Ketosis is a metabolic state in which the body, due to a significantly reduced intake of carbohydrates, shifts its primary energy source from glucose to ketone bodies, i.e., beta-hydroxybutyrate (BHB), acetoacetate, and acetone, which are produced in the liver from fatty acids. This review examines the mechanisms by which ketone bodies, particularly BHB, mitigate cardiovascular risk. We focus mainly on the anti-inflammatory and antioxidative properties of BHB and summarize recent evidence to highlight the clinical relevance of ketosis in cardiometabolic health.
Inappropriate Diet Exacerbates Metabolic Dysfunction-Associated Steatotic Liver Disease via Abdominal Obesity
Authors: Xiang Minghui , Tian Xiaoli , Wang Hui , Gan Ping , Zhang Qian
Published: December 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) represents a refined categorization of non-alcoholic fatty liver disease (NAFLD), highlighting the intricate relationship between hepatic steatosis and metabolic dysfunction. Abdominal obesity (AO), a key diagnostic criterion for metabolic dysfunction, predominantly results from inappropriate diet and unhealthy dietary habits. To comprehensively investigate which dietary factors contribute to MASLD through AO and to understand the underlying biological mechanisms, we initially conducted a systematic review of meta-analysis articles in the PubMed database from the past decade, summarizing dietary factors that affect AO. Subsequently, we conducted targeted searches in the PubMed database for these dietary factors and provided a narrative review of the mechanisms of how these dietary factors lead to AO and how AO exacerbates MASLD. A diet characterized by excessive intake of energy, carbohydrates, fructose, or ultra-processed foods (UPFs) is considered inappropriate. Inappropriate diet leads to the formation of MASLD and AO by enhancing pathways such as de novo lipid synthesis (DNL) in the liver, insulin resistance (IR), gut–liver dysfunction, and inflammation. Dietary interventions for inappropriate diets can effectively intervene in and improve MASLD and AO. The mechanism of inappropriate diet on abdominal fat deposition is through excessive energy or the activation of the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1) to increase endocortisol secretion. Then, the excessive accumulation of visceral fat facilitates a rapid and augmented flux of free fatty acids (FFAs) to the liver and initiates a series of deleterious effects, including oxidative stress (OS), endoplasmic reticulum stress (ERS), activation of protein kinase C (PKC) pathways, and inflammation. Additionally, FFAs may mediate excessive lipid deposition and hepatocellular damage through the action of hormones. These pathways to liver damage exacerbate MASLD and progression to metabolic dysfunction-associated steatohepatitis (MASH) and fibrosis. Furthermore, investigating other potential mechanisms by which AO may influence MASLD could offer new recommendations for the treatment guidelines of MASLD.
Enhancing Nursing Students’ Attitudes Toward Older Adults Through an Artificial Intelligence Virtual Simulation: A Mixed-Method Design
Authors: White Anne , Maguire Mary Beth , Brown Austin
Published: December 2024

Background/Objectives: The projected increase from 58 million older adults in 2022 to 82 million by 2050 in the United States highlights the urgency of preparing nursing students to care for this aging population. However, studies reveal negative attitudes among nursing students toward older adults. A three-phased educational intervention that included an artificial intelligence (AI)-driven virtual simulation was implemented to address this. AI-generated simulations promise to expose marginalized groups and strengthen future nurses’ knowledge, skills, and attitudes. Methods: A convergent mixed-method design was used to measure the change in nursing students’ attitudes toward older adults, as measured by the UCLA Geriatrics Attitudes Survey and a Guided Reflection survey after participating in an Artificial Intelligence in Education learning event (n = 151). Results: The results indicate that post-intervention scores (M = 35.07, SD = 5.34) increased from pre-intervention scores (M = 34.50, SD = 4.86). This difference was statistically significant at the 0.10 significance level (t = 1.88, p = 0.06). The qualitative analysis indicated that the attitudes impacted were challenging and overcoming ageism, increased empathy and patience, and enhanced communication skills. Conclusions: Utilizing artificial intelligence technology during educational events effectively yields measurable learning outcomes. Cultivating positive attitudes toward older adults is essential for competent care in an aging society. This study was prospectively approved by the university’s Institutional Review Board (IRB) on 30 July 2021, IRB-FY22-3.
Increasing Self-Efficacy for the Management of Patients with Type 2 Diabetes Through an Advanced Practice Education Program for Primary Care Professionals
Authors: Yunis Bushra , Echevarría-Pérez Paloma , Morante Juan Jose Hernandez , Morales-Moreno Isabel
Published: December 2024

Background/Objectives: Previous studies have shown that primary care (PC) professionals have a low knowledge about the management of patients with type 2 diabetes, despite being one of the most common chronic diseases. The objective of this study is to analyze the impact of an educational program for health professionals on the metabolic control of their patients diagnosed with type 2 diabetes. Methods: This work follows a quasi-experimental longitudinal design following a double perspective. First, an educational intervention was conducted on primary care health professionals. Previous diabetes knowledge was evaluated on 157 PC health professionals. Those with lower knowledge scores (<3.5 out of 7) were allocated to the intervention group. An 8-week advanced education program was conducted on 77 PC health professionals. Self-efficacy and quality of care were evaluated. Second, a prospective cohort study was conducted to evaluate changes in metabolic parameters in their patients with type 2 diabetes. A total of 4099 patients with type 2 diabetes attending PC services were divided depending on the formation of health professionals. Biochemical and other clinical parameters were determined at baseline and after 12 months; the study was allocated in the Primary Health Centers of Meuhedet North District (Israel), from January 2022 to June 2023. Changes from the baseline were compared using ANOVA. Additionally, a mixed-effect model was conducted to capture variability within primary care staff and between groups of patients. Results: The education program significantly improved health staff knowledge (p < 0.001) and all dimensions of self-efficacy (p < 0.001 in all cases). These improvements were mirrored in patients’ outcomes, since those managed by health professionals attending the advanced practice education showed, after 6 months, better glucose (p < 0.001), HbA1c (p < 0.001), and eGFR (p = 0.006) levels. Conclusions: The advanced practice education program oriented to PC professionals was able to significantly improve their self-efficacy and perceived quality of care, which induced a significant effect on metabolic markers of patients with type 2 diabetes. Overall, the data reinforce the usefulness of advanced education programs, especially in chronic complex diseases like type 2 diabetes.
Placental Macrovascular Pattern from Pregnancies with Maternal Hypertensive and Fetal Growth Capacity Complications
Authors: Makhambetova Kamilya , Kamyshanskiy Yevgeniy , Ponamareva Olga , Amirbekova Zhanna , Oshakhtiyeva Nazerke , Kunanbaeva Saule
Published: December 2024

Histomorphometric measurements of the wall thickness and internal diameter of the macrovessels of the chorionic villi of placentas from pregnancies complicated by preeclampsia or fetal growth restriction in comparison with normotensive pregnancy. Methods: The research included placentas from singleton pregnancies complicated by preeclampsia and/or fetal growth restriction, women delivered in medical institutions in Karaganda city (Kazakhstan). Placentas were divided into three groups: PE (n = 59), isolated FGR (n = 24), and PE with FGR (n = 41). The control group consisted of normotensive pregnancies, compared by gestation period. Placental examination and selection of placental tissue fragments were carried out in accordance with the consensus recommendations of the Amsterdam Placental Workshop Group. The sections were stained with hematoxylin and eosin and Masson trichrome. Morphometric measurements were performed using ImageJ software version 1.52p. Results: Our data showed that, in the PE group, there was a significant decrease in the wall thickness of the proximal and distal vessels with an increase in internal diameter compared with the control group (p < 0.01). In the PE + FGR group, there was a thickening of the wall of the proximal part of the vessels with a decrease in their lumen and a decrease in the wall thickness of the vessels with an increase in the lumen in the distal part compared with the control group (p < 0.01). Conclusions: Two histopatterns of placental macrovessels in preeclampsia were revealed: the histophenotype of diffuse (proximal and distal) ectatic macroangiopathy with a thin vascular wall with a decrease in the thickness of the muscle layer and the histophenotype of proximal fibromuscular sclerosis with vascular obliteration/spasm and distal ectatic macroangiopathy. We believe that significant structural differences in vascular remodeling may reflect the different temporal and spatial nature of the pathological factor. Future research is needed to investigate the associations between histopatterns of placental vascular remodeling in preeclampsia and long-term perinatal/maternal outcomes.
Nanoencapsulation of Achyrocline satureioides (Lam) DC—Essential Oil and Controlled Release: Experiments and Modeling
Authors: da Silva Caroline G. F. , Petró Rafaela R. , de Castro Jéssica H. , Almeida Rafael N. , Cassel Eduardo , Vargas Rubem M. F.
Published: December 2024

Background/Objectives: Degradation by physical and chemical agents affects the properties of essential oils; therefore, this study aimed to protect the volatile compounds present in essential oils through biopolymer encapsulation. Methods: The Achyrocline satureioides (Lam) DC. essential oil was obtained by steam distillation at 2.5 bar. The nano-sized physical coating of the active oil core resulted in an optimal polymer/oil ratio of 1:3 and particle diameter of 178 nm. The particle morphology was evaluated using scanning electron microscopy and transmission electron microscopy. The inclusion of the essential oil in the polymer was confirmed using thermogravimetric analysis. Results: The pH of the formulation remained stable for 90 days, and controlled release and encapsulation efficiencies were evaluated. Formulations were evaluated using the perfumery radar technique, which indicated a predominantly woody profile. The diffusion of fragrant compounds in the air was assessed over time and mathematically modeled. Conclusions: The produced nanostructures were efficient for the controlled release of volatile compounds from the essential oil of Achyrocline satureioides.