bluuebunny/crossref_metadata_2025 huggingface dataset:
Current and Future Salinity Intrusion in the South-Western Coastal Region of Bangladesh
Authors: Ashrafuzzaman Md , Artemi Cerdà , Santos Filipe Duarte , Schmidt Luísa
Published: May 2022

The southwestern coastal regions of Bangladesh (SWCRB) are highly exposed to saltwater intrusions brought about through cyclones and storm surges. These salinity intrusions are contributing to soil and water salinity in the coastal areas. This study aimed to determine the impact of these salinity intrusions on the quality of water and soil in three vulnerable coastal areas. In this investigation, water and soil samples were collected and analysed for pH, electrical conductivity (EC) and other trace elements. The analysis found many of the parameters to be higher than the recommended values. The study found that in soil samples there was a significant correlation between OM and ECe dS/m, as well as K and TN; and a highly significant correlation between TN and OM. This study further examined the historical salinity data at low and high tides to determine any patterns occurring alongside storm surges and cyclones. Water salinity statistics were obtained from the three locations of the Bangladesh Water Development Board (BWDB), which neighbours the study area. A Digital Evaluation Model (DEM) predicts the salinity induced by storm gushes in the corresponding impacted zones. Lastly, the study compared projections for future storm surges at current and predicted sea levels. Potential storm gushes circumstances from 1 to 9 m can impact up to 33% of the nation and 97% of the Shyamnagar Upazila. The occurrence of cyclone-related storms will increase and make cultivation and settlement in the region difficult. The predicted sea-level rises and saltwater contamination will intensify the adverse effects of salinity.
Women’s Empowerment as an Outcome of NGO Projects: Is the Current Approach Sustainable?
Authors: Al Hakim Ghenwa , Bastian Bettina Lynda , Ng Poh Yen , Wood Bronwyn P.
Published: May 2022

The area of women’s empowerment has attracted increasing attention among a wide range of interest groups, from authors to researchers to feminist scholars and non-governmental organizations (NGOs). This paper aims to identify the diverse understandings of women’s empowerment in the literature and to discuss empirical evidence from NGO projects in the field. A systematic literature review is employed that includes an analysis of relevant high-quality articles and research papers published in the Scopus database, as well as those produced by United Nations (UN) bodies and well-published authors. The findings highlighted four common understandings of women’s empowerment including granting women a voice, challenging existing power structures, the radical transformation of lives and livelihoods, and gender mainstreaming. The findings of these empirical studies on the role of NGOs in this field revealed understandings limited to granting women a voice and gender mainstreaming, thus reflecting the fact that limited knowledge of women’s empowerment hampers the ability of NGOs to serve women’s advancement and sustainable development. Furthermore, and given that these approaches and understandings still fall short of achieving social inclusion for women, it is recommended that these NGOs discharge their efforts toward initiating systemic change to actually sustain female empowerment in the communities in which they are active.
A Clustering Ensemble Method of Aircraft Trajectory Based on the Similarity Matrix
Authors: Chu Xiao , Tan Xianghua , Zeng Weili
Published: May 2022

Performing clustering analysis on a large amount of historical trajectory data can obtain information such as frequent flight patterns of aircraft and air traffic flow distribution, which can provide a reference for the revision of standard flight procedures and the optimization of the division of airspace sectors. At present, most trajectory clustering uses a single clustering algorithm. When other processing remains unchanged, it is difficult to improve the clustering effect by using a single clustering method. Therefore, this paper proposes a trajectory clustering ensemble method based on a similarity matrix. Firstly, a stacked autoencoder is used to learn a small number of features that are sufficiently representative of the trajectory and used as the input to the subsequent clustering algorithm. Secondly, each basis cluster is used to cluster the data set, and then a consistent similarity matrix is obtained by using the clustering results of each basis cluster. On this basis, using the deformation of the matrix as the distance matrix between trajectories, the agglomerative hierarchical clustering algorithm is used to ensemble the results of each basis cluster. Taking the Nanjing Lukou Airport terminal area as an example, the experimental results show that integrating multiple basis clusters eliminates the inherent randomness of a single clustering algorithm, and the trajectory clustering results are more robust.
Impact of Cropping Pattern and Climatic Parameters in Lower Chenab Canal System—Case Study from Punjab Pakistan
Authors: Mujtaba Ahmad , Nabi Ghulam , Masood Muhammad , Iqbal Mudassar , Asfahan Hafiz M. , Sultan Muhammad , Majeed Faizan , Hensel Oliver , Nasirahmadi Abozar
Published: May 2022

In Pakistan, groundwater resources are depleting at an alarming rate due to intensive pumping, shifting of cropping patterns, and climate change vulnerability. The present study is aimed at investigating groundwater stress in the command area of Lower Chenab Canal (LCC) and associated branch canals. Groundwater stress is determined by considering the cropping patterns, surface water availability, groundwater levels, climatic variation, and crop water requirement (CWR) in the LCC command area. The climatic data is obtained from the Pakistan Meteorological Department (PMD) from 1990 to 2020. The records of temporal variation in cropping patterns are obtained from the Crop Reporting Service (CRS), Directorate of Agriculture, Lahore for the 1995–2020 period and classified according to Rabi season (November to April) and Kharif season (May to October). The LCC surface water flows data and groundwater levels are collected from the Punjab Irrigation Department (PID) Lahore from 2003 to 2018 and from 1995 to 2016, respectively. The CWR is estimated using the Cropwat 8.0 model and groundwater levels are estimated using the Inverse Distance Weighted (IDW) tool of ArcGIS software. It has been determined that Faisalabad, Sheikhupura, and Toba Tek Singh are highly groundwater stress cities having an average drawdown rate of 0.58 m/year. The surface water availability is also decreased from 7.75 to 4.81 billion cubic meters (Bm3) for the Kharif season whilst 4.17 to 2.63 Bm3 for the Rabi season. This study concluded that due to severe conditions in highly stressed areas, policy planners, decision-makers, and stakeholders should sincerely take some steps for maintaining groundwater levels either by capacity building workshops for the farmers or limiting the number of tubewells.
Effect of Mepiquat Chloride on Phenology, Yield and Quality of Cotton as a Function of Application Time Using Different Sowing Techniques
Authors: Murtza Khadija , Ishfaq Muhammad , Akbar Nadeem , Hussain Saddam , Anjum Shakeel Ahmad , Bukhari Najat A. , AlGarawi Amal Mohamed , Hatamleh Ashraf Atef
Published: May 2022

Mepiquat chloride (MC) is a plant growth regulator used to manage the rampant vegetative growth of cotton. A two-year field experiment was conducted at the Postgraduate Agricultural Research Station, University of Agriculture, Faisalabad, Pakistan, during 2017 and 2018 to investigate the influence of MC applied at different times on phenology, morphology, lint yield and quality of cotton cultivated using different sowing techniques. MC was applied 50 days after sowing (DAS), 60 DAS and 70 DAS to cotton planted in flat fields (flat sowing), ridges (ridge sowing) and beds (bed sowing). The interactive effect of MC application time and sowing technique did not influence crop phenology, morphology, and lint yield and quality. It was revealed that the crop planted on beds took fewer days to flower (10%) as compared to that on the flat field, and the bed-sown crop produced a higher number of opened bolls (60%) and was characterized by a higher boll weight (32%) and seed cotton yield (50%) in comparison to the flat-sown crop. A late application of MC (at 70 DAS) caused a significant reduction in the time to flowering (8%), with a simultaneous increase in the number of opened bolls (60%), boll weight (32%), ginning out turn (8%) and lint yield (27%) as compared to MC application at 50 DAS. In terms of lint quality, cotton planted on beds had better fiber uniformity (8%) compared to that on the flat field, while MC applied at 70 DAS produced better fiber fineness by 27% in comparison to MC applied earlier. Overall, cotton planting on beds and MC application at 70 DAS may help improve cotton yield and fiber quality and may help in the mechanical picking of cotton.
Comparison of Nutritional Profiles of Super Worm (Zophobas morio) and Yellow Mealworm (Tenebrio molitor) as Alternative Feeds Used in Animal Husbandry: Is Super Worm Superior?
Authors: Dragojlović Danka , Đuragić Olivera , Pezo Lato , Popović Ljiljana , Rakita Slađana , Tomičić Zorica , Spasevski Nedeljka
Published: May 2022

Edible insects are acknowledged as a valuable nutritional source and promising alternative to traditional feed ingredients, while the optimization of rearing conditions is required for their wider utilization in the animal feed industry. The main goal of this study was to compare and optimize the rearing conditions of the two species’ larvae and identify the most favorable nutritive composition of the full-fat larval meal. For that purpose, Tenebrio molitor (TM) and Zophobas morio (ZM) were reared on three different substrates and harvested after three time periods. An artificial neural network (ANN) with multi-objective optimization (MOO) was used to investigate the influence between the observed parameters as well as to optimize and determine rearing conditions. The optimization of the larval rearing conditions showed that the best nutritive composition of full-fat larval meal was obtained for ZM larvae reared on a mixture of cabbage, carrot and flaxseed and harvested after 104 days. The best nutritive composition contained 39.52% protein, 32% crude fat, 44.01% essential amino acids, 65.21 mg/100 g Ca and 651.15 mg/100 g P with a favorable ratio of 1.5 of n6/n3 fatty acids. Additionally, the incorporation of flaxseed in the larval diet resulted in an increase in C18:3n3 content in all samples.
Pathology and Prevalence of Antibiotic-Resistant Bacteria: A Study of 398 Pet Reptiles
Authors: Cristina Romeo T. , Kocsis Rudolf , Dégi János , Muselin Florin , Dumitrescu Eugenia , Tirziu Emil , Herman Viorel , Darău Aurel P. , Oprescu Ion
Published: May 2022

Reptiles are potential reservoirs of bacteria that could be transmitted, thus becoming a zoonotic hazard. (1) Background: This three-year investigation surveyed the pathological status of 398 pet reptiles: chelonians, snakes (venomous/non-venomous), and lizards. The main pathological entities found were related to the skin, the sensory organs, the digestive system, the respiratory system, the cardiovascular system, the urinary system, the genitalia, the osteo–muscular tract, surgical issues, tumors, and intoxications. (2) Methods: In 25 individuals treated with antibiotics, no clinical healing was recorded, for this reason, an antimicrobial resistance profile analysis of the 43 samples gathered was processed. An antibiogram was performed using the VITEK®2 ID-GP (bio-Mérieux, Marcy l’Etoile, France) automated platform, with 22 bacterial strains being isolated. (3) Results: The statistics (ANOVA) revealed that the most common disease category was diseases of the digestive system, followed by diseases of the skin, respiratory system, nervous system, and reproductive system. A significant correlation (p < 0.01) between disease incidence and reptile species was reported, with correlations found between all species and diseases diagnosed. The most common bacteria isolated were Enterococcus faecalis, Pseudomonas aeruginosa, Stenotrophomas (Xanthomonas) maltophilia, Escherichia coli, Klebsiella oxytoca, and Salmonella spp., but Beta-hemolytic Streptococcus, Staphylococcus aureus, Citrobacter spp., and Proteus spp. were also identified. (4) Conclusions: These microorganisms revealed degrees of resistance against penicillins, cephalosporins, macrolides, lincosamides, aminoglycosides, and tetracyclines. The animals can be categorized according to their sensitivity to diseases in the following order (most sensitive to least sensitive): chelonians, venomous snakes, non-venomous snakes, and lizards.
Animal Protection, Law Enforcement, and Occupational Health: Qualitative Action Research Highlights the Urgency of Relational Coordination in a Medico-Legal Borderland
Authors: Rault Dawn , Adams Cindy L. , Springett Jane , Rock Melanie J.
Published: May 2022

Across Canada and internationally, laws exist to protect animals and to stop them from becoming public nuisances and threats. The work of officers who enforce local bylaws protects both domestic animals and humans. Despite the importance of this work, research in this area is emergent, but growing. We conducted research with officers mandated to enforce legislation involving animals, with a focus on local bylaw enforcement in the province of Alberta, Canada, which includes the city of Calgary. Some experts regard Calgary as a “model city” for inter-agency collaboration. Based on partnerships with front-line officers, managers, and professional associations in a qualitative multiple-case study, this action-research project evolved towards advocacy for occupational health and safety. Participating officers spoke about the societal benefits of their work with pride, and they presented multiple examples to illustrate how local bylaw enforcement contributes to public safety and community wellbeing. Alarmingly, however, these officers consistently reported resource inadequacies, communication and information gaps, and a culture of normalized disrespect. These findings connect to the concept of “medico-legal borderlands,” which became central to this study. As this project unfolded, we seized upon opportunities to improve the officers’ working conditions, including the potential of relational coordination to promote the best practices.
Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells
Authors: Mollichella Marie-Laure , Mechin Violaine , Royer Dany , Pageat Patrick , Asproni Pietro
Published: May 2022

The olfactory mucosa contains olfactory ecto-mesenchymal stem cells (OE-MSCs) which show stemness features, multipotency capabilities, and have a therapeutic potential. The OE-MSCs have already been collected and isolated from various mammals. The aim of this study was to evaluate the feasibility of collecting, purifying and amplifying OE-MSCs from the cat nasal cavity. Four cats were included in the study. Biopsies of olfactory mucosa were performed on anesthetized animals. Then, the olfactory OE-MSCs were isolated, and their stemness features as well as their mesodermal differentiation capabilities were characterized. Olfactory mucosa biopsies were successfully performed in all subjects. From these biopsies, cellular populations were rapidly generated, presenting various stemness features, such as a fibroblast-like morphology, nestin and MAP2 expression, and sphere and colony formation. These cells could differentiate into neural and mesodermal lineages. This report shows for the first time that the isolation of OE-MSCs from cat olfactory mucosa is possible. These cells showed stemness features and multilineage differentiation capabilities, indicating they may be a promising tool for autologous grafts and feline regenerative medicine.
Evaluation of Glycyrrhizic Acid Therapeutic Effect and Safety in Mycoplasma gallisepticum (HS Strain)-Infected Arbor Acres Broilers
Authors: Hu Fuli , Luo Ronglong , Duan Shuwen , Guo Qiao , Wang Lulu , Jiang Guangyang , Fan Changyong , Zou Mengyun , Wang Tengfei , Wang Yingjie , Sun Yingfei , Peng Xiuli
Published: May 2022

This study was conducted to evaluate the therapeutic effects and safety of GA in MG-infected broilers. Our results showed that the minimum inhibitory concentration of GA was 31.25 μg/mL. Moreover, GA inhibited the expression of MG adhesion protein (pMGA1.2) in the broilers’ lungs. GA treatment clearly decreased the morbidity of CRD and mortality in the MG-infected broilers. Compared with the model group, GA treatment significantly decreased gross air sac lesion scores and increased average weight gain and feed conversion rate in the MG-infected broilers. Histopathological examination showed GA treatment attenuated MG-induced trachea, immune organ and liver damage in the broilers. Moreover, GA treatment alone did not induce abnormal morphological changes in these organs in the healthy broilers. Compared with the model group, serum biochemical results showed GA treatment significantly decreased the content of total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, total bilirubin, creatinine, uric acid, total cholesterol, and increased the content of albumin/globulin, alkaline phosphatase, apolipoprotein B and apolipoprotein A-I. In conclusion, GA displayed a significant therapeutic efficacy regarding MG infection and had no adverse effects on the broilers (100 mg/kg/d).
Co-Application of Tetramycin and Matrine Improves Resistance of Kiwifruit against Soft Rot Disease and Enhances Its Quality and Amino Acids
Authors: Zhang Cheng , Li Wenzhi , Long Youhua , Su Yue , Zhang Qinghai
Published: May 2022

Soft rot disease caused by Botryosphaeria dothidea and Phomopsis sp. is the most serious fungal disease of the kiwifruit production area in southwest China. In this work, the role of the co-application of tetramycin and matrine in the resistance of kiwifruit fruits against soft rot disease and its effects on development, quality and amino acids of kiwifruit fruits were investigated. The results indicate that matrine exhibited an outstanding toxicity against B. dothidea RF-1 and Phomopsis sp. RF-2 with EC50 values of 0.442 and 0.332 mg kg−1. The foliar co-application of 0.3% tetramycin aqueous solutions (AS) 5000-fold liquid + 0.5% matrine AS 1000-fold liquid could effectively control soft rot disease with a control efficacy of 82.68%, which was significantly (p < 0.05) higher than 75.19% of 0.3% tetramycin AS 5000-fold liquid and significantly (p < 0.01) higher than 68.50% of 0.5% matrine AS 500-fold liquid. Moreover, the co-application of tetramycin and matrine was more effective than tetramycin or matrine alone in improving disease resistance, quality and amino acids of kiwifruit fruits. This study highlights that the co-application of tetramycin and matrine can be used as a practicable, cost-effective and environmentally friendly candidate or alternative approach for controlling soft rot disease of kiwifruit.
Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin
Authors: van Gent Miriam E. , van der Reijden Tanny J. K. , Lennard Patrick R. , de Visser Adriëtte W. , Schonkeren-Ravensbergen Bep , Dolezal Natasja , Cordfunke Robert A. , Drijfhout Jan Wouter , Nibbering Peter H.
Published: May 2022

Recently, using a deep learning approach, the novel antibiotic halicin was discovered. We compared the antibacterial activities of two novel bactericidal antimicrobial agents, i.e., the synthetic antibacterial and antibiofilm peptide (SAAP)-148 with this antibiotic halicin. Results revealed that SAAP-148 was more effective than halicin in killing planktonic bacteria of antimicrobial-resistant (AMR) Escherichia coli, Acinetobacter baumannii and Staphylococcus aureus, especially in biologically relevant media, such as plasma and urine, and in 3D human infection models. Surprisingly, SAAP-148 and halicin were equally effective against these bacteria residing in immature and mature biofilms. As their modes of action differ, potential favorable interactions between SAAP-148 and halicin were investigated. For some specific strains of AMR E. coli and S. aureus synergism between these agents was observed, whereas for other strains, additive interactions were noted. These favorable interactions were confirmed for AMR E. coli in a 3D human bladder infection model and AMR S. aureus in a 3D human epidermal infection model. Together, combinations of these two novel antimicrobial agents hold promise as an innovative treatment for infections not effectively treatable with current antibiotics.
An Evolutionary Conservation and Druggability Analysis of Enzymes Belonging to the Bacterial Shikimate Pathway
Authors: Frlan Rok
Published: May 2022

Enzymes belonging to the shikimate pathway have long been considered promising targets for antibacterial drugs because they have no counterpart in mammals and are essential for bacterial growth and virulence. However, despite decades of research, there are currently no clinically relevant antibacterial drugs targeting any of these enzymes, and there are legitimate concerns about whether they are sufficiently druggable, i.e., whether they can be adequately modulated by small and potent drug-like molecules. In the present work, in silico analyses combining evolutionary conservation and druggability are performed to determine whether these enzymes are candidates for broad-spectrum antibacterial therapy. The results presented here indicate that the substrate-binding sites of most enzymes in this pathway are suitable drug targets because of their reasonable conservation and druggability scores. An exception was the substrate-binding site of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, which was found to be undruggable because of its high content of charged residues and extremely high overall polarity. Although the presented study was designed from the perspective of broad-spectrum antibacterial drug development, this workflow can be readily applied to any antimicrobial target analysis, whether narrow- or broad-spectrum. Moreover, this research also contributes to a deeper understanding of these enzymes and provides valuable insights into their properties.
Integrative Comparative Assessment of Cold Acclimation in Evergreen and Deciduous Iris Species
Authors: Shao Lingmei , Xu Tong , Wang Xiaobin , Zhang Runlong , Wang Xiuyun , Ren Ziming , Zhang Jiaping , Xia Yiping , Li Danqing
Published: May 2022

Cold acclimation (CA) is a strategy which plants have evolved to increase freezing tolerance. Global climate change could obstruct CA and raise the probability of winter injury, especially for evergreens. Hence, understanding the regulatory mechanism of CA is crucial to improve freezing tolerance in evergreen plants. A comparative study on a pair of closely related evergreen and deciduous iris species in response to cold through CA was conducive to uncovering and complementing the knowledge of CA. We investigated morphological, physiological and biochemical changes, as well as the expression of associated genes in the functional leaves of both iris species from natural CA to deacclimation. Briefly, fast and strong CA in the evergreen iris might cause early expressions of BAM1, NCED3, GPX6, etc., which leads to strong enzyme activity of starch degradation, abscisic acid biosynthesis and reactive oxygen species scavenging. Additionally, genes belonging to the antioxidant system were mainly induced during deacclimation. These results suggest that interspecies differences in the leaf freezing tolerance of irises are associated with the rate and degree of CA, which activates multiple signaling networks with complex interactions and induces the transcription of cold-responsive genes. Moreover, the ICE–CBF–COR signaling cascade may integrate and initiate diverse cold-responsive pathways during CA of the evergreen iris. The findings of this study provide valuable insight to further research on CA mechanisms and implicate genes which could support breeding strategies in herbaceous perennials under climate changes.
Jelly Fig (Ficus awkeotsang Makino) Exhibits Antioxidative and Anti-Inflammatory Activities by Regulating Reactive Oxygen Species Production via NFκB Signaling Pathway
Authors: Lin Meng-Jin , Lin Ping , Wen Kuo-Ching , Chiang Hsiu-Mei , Lu Mei-Chun
Published: May 2022

Antioxidant and anti-inflammatory activities of Ficus awkeotsang Makino extract (FAE) on Hs68 fibroblasts and BALB/c nude-mouse models are evaluated in this study. FAE was found to be non-toxic and showed high levels of DPPH, H2O2, and hydroxyl radical scavenging abilities; a ferrous chelating capacity; as well as ferric-reducing antioxidant capability. The antioxidant activity of FAE was strongly associated with polyphenolic content (flavonoids at 10.3 mg QE g−1 and total phenol at 107.6 mg GAE g−1). The anti-inflammatory activity of FAE and the underlying molecular mechanisms were also investigated. The a* value of the mouse dorsal skin after treatment with FAE at 1.5 mg/mL in addition to chronic UVB exposure was found to decrease by 19.2% during a ten-week period. The anti-inflammatory effect of FAE was evidenced by the decreased accumulation of inflammatory cells and skin thickness. Expression levels of UVB-induced inflammatory proteins, including ROS, NF-κB, iNOS, COX-2, and IL-6, were significantly reduced upon FAE treatment in vitro and in vivo. Collectively, our results suggest that the inhibition of ROS and UVB-induced activation of the NF-κB downstream signaling pathway by FAE, indicating considerable potential as a versatile adjuvant against free radical damage in pharmaceutical applications.
Effects of the SGLT2 Inhibition on Cardiac Remodeling in Streptozotocin-Induced Diabetic Rats, a Model of Type 1 Diabetes Mellitus
Authors: Rosa Camila Moreno , Campos Dijon Henrique Salome , Reyes David Rafael Abreu , Damatto Felipe Cesar , Kurosaki Lucas Yamada , Pagan Luana Urbano , Gomes Mariana Janini , Corrêa Camila Renata , Fernandes Ana Angelica Henrique , Okoshi Marina Politi , Okoshi Katashi
Published: May 2022

Clinical trials have shown that sodium glucose co-transporter 2 (SGLT2) inhibitors improve clinical outcomes in diabetes mellitus (DM) patients. As most studies were performed in Type 2 DM, the cardiovascular effects of SGLT2 inhibition still require clarification in Type 1 DM. We analyzed the effects of SGLT2 inhibitor dapagliflozin on cardiac remodeling in rats with streptozotocin-induced diabetes, an experimental model of Type 1 DM. Methods: Male Wistar rats were assigned into four groups: control (C, n = 14); control treated with dapagliflozin (C + DAPA, n = 14); diabetes (DM, n = 20); and diabetes treated with dapagliflozin (DM + DAPA, n = 20) for 8 weeks. Dapagliflozin dosage was 5 mg/kg/day. Statistical analyses: ANOVA and Tukey or Kruskal–Wallis and Dunn. Results: DM + DAPA presented decreased blood pressure and glycemia and increased body weight compared to DM (C 507 ± 52; C + DAPA 474 ± 50; DM 381 ± 52 *; DM + DAPA 430 ± 48 # g; * p < 0.05 vs. C; # p < 0.05 vs. C + DAPA and DM + DAPA). DM echocardiogram presented left ventricular and left atrium dilation with impaired systolic and diastolic function. Cardiac changes were attenuated by dapagliflozin. Myocardial hydroxyproline concentration and interstitial collagen fraction did not differ between groups. The expression of Type III collagen was lower in DM and DM + DAPA than their controls. Type I collagen expression and Type I-to-III collagen ratio were lower in DM + DAPA than C + DAPA. DM + DAPA had lower lipid hydroperoxide concentration (C 275 ± 42; C + DAPA 299 ± 50; DM 385 ± 54 *; DM + DAPA 304 ± 40 # nmol/g tissue; * p < 0.05 vs. C; # p < 0.05 vs. DM) and higher superoxide dismutase and glutathione peroxidase activity than DM. Advanced glycation end products did not differ between groups. Conclusion: Dapagliflozin is safe, increases body weight, decreases glycemia and oxidative stress, and attenuates cardiac remodeling in an experimental rat model of Type 1 diabetes mellitus.
Cybersecurity Vulnerabilities in Off-Site Construction
Authors: Nyamuchiwa Kudakwashe , Lei Zhen , Aranas Clodualdo
Published: May 2022

Industry 4.0 is seeking to advance traditional construction practices towards more efficient and internet of things (IoT)-based construction practices, such as offsite construction. Offsite construction (OSC) allows for the simultaneous fabrication of building modules and onsite work. Integrating IoT technologies in construction practice is projected to improve the industry’s growth. However, there is an increase in cybersecurity vulnerabilities. Cyber threats are becoming more disruptive and targeted, resulting in monetary and infrastructure losses. Furthermore, the COVID pandemic and the instability in Europe have seen over 100% increases in cyber-attacks, and most industries have weak cybersecurity protocols. The adoption of cybersecurity frameworks in the construction industry is sluggish, and the existing security frameworks fall short in addressing the needs of the industry. This paper gives a concise review of the offsite construction value chain vulnerabilities. We explore the existing cybersecurity frameworks and identify their limitations. Cybersecurity is presented as one of the most crucial components that has received little or no attention in OSC. The future of OSC is promising with the incorporation of Industry 4.0 technologies; however, its development needs to consider more proactive security approaches and management techniques that are adapted to the current hostile cyber landscape.
In Vitro Antioxidant, Antibacterial and Mechanisms of Action of Ethanolic Extracts of Five Tunisian Plants against Bacteria
Authors: Nefzi Khaoula , Ben Jemaa Mariem , Baraket Mokhtar , Dakhlaoui Sarra , Msaada Kamel , Nasr Zouheir
Published: May 2022

Pistacia lentiscus, Rosmarinus officinalis, Erica multiflora, Calicotome villosa, and Phillyrea latifolia were considered important medicinal herbs and were used to treat various ailments. The present study was designed to evaluate the antioxidant and antimicrobial activities of ethanolic extracts (EEs). P. lentiscus and R. officinalis were the richest species in phenolic compounds. Similarly, both species showed the highest values of flavonoids. While the EEs of P. lentiscus, E. multiflora, and C. villosa had higher amounts of tannins. These phenolic compounds were evaluated by two different tests, namely diphenyl picrylhydrazyl (DPPH) and ferric iron-reducing power (FRAP). The IC50 values were found to be significant (p < 0.05) for P. lentiscus and E. multiflora. Similarly, both plants showed the highest ferric-reducing antioxidant power (FRAP). This study has been conducted to evaluate the antibacterial potential of EEs against selected bacteria—Gram-positive bacteria (Staphylococcus aureus ATCC 29213, Listeria monocytogenes ATCC 7644) and Gram-negative bacteria (Escherichia coli ATCC 8739, Salmonella typhimurium NCTC 6017)—and determine their modes of action. The ethanolic extracts inhibited bacterial growth by producing concentration-dependent zones of inhibition. Treatment with these extracts at their minimum inhibitory concentrations (MICs) showed a significant reduction (p < 0.05) in the viability of bacteria. The extracts did not induce total lysis. Bacteria organisms treated with EEs at MICs showed a significant (p < 0.05) loss of tolerance to NaCl (5%). Our results highlighted the use of plant extracts as natural antibacterials that can be safely used in health care and led to the understanding of the antibacterial mechanism of plant extracts.
Ultrafast Detection of SARS-CoV-2 Spike Protein (S) and Receptor-Binding Domain (RBD) in Saliva Using Surface-Enhanced Raman Spectroscopy
Authors: Abdullah Mohammed Ba , Dab Chahinez , Almalki Mohammed , Alnaim Abdullah , Abuzir Alaaedeen , Awada Chawki
Published: May 2022

Controlling contagious diseases necessitates using diagnostic techniques that can detect infection in the early stages. Although different diagnostic tools exist, there are still challenges related to accuracy, rapidity, cost-effectiveness, and ease of use. Surface-enhanced Raman spectroscopy (SERS) is a rapid, simple, less expensive, and accurate method. We continue our previous work published on SERS detection of the SARS-CoV-2 receptor-binding domain (RBD) in water. In this work, we replace water with saliva to detect SARS-CoV-2 proteins at very low concentrations and during a very short time. We prepared a very low concentration of 10−9 M SARS-CoV-2 spike protein (S) and SARS-CoV-2 receptor-binding domain (RBD) in saliva to mimic a real case scenario. Then, we drop them on a SERS substrate. Using modified SERS measurements on the control and the sample containing the biomolecules, confirmed the sensitivity of the target identification. This technique provides different diagnostic solutions that are fast, simple, non-destructive and ultrasensitive. Simulation of the real-world of silicon wire covered with silver and gold, were performed using an effective and accurate tool, COMSOL Multiphysics software, for the enhancement properties study.
Changes in Propeller Shaft Behavior by Fluctuating Propeller Forces during Ship Turning
Authors: Lee Ji-Woong , Vuong Quang Dao , Jeong Byongug , Lee Jae-ung
Published: May 2022

It is known that a ship’s shafting system can be adversely affected by hull deformation, variations in the engine power, the propeller load, and eccentric propeller thrusts, thereby increasingly affecting the behavior of the shaft’s movement. A deformed shafting system may also lead to a potential risk of bearing damage by causing a change in the local load of the rear part of the after-stern tube bearing of the propeller shaft. With this concern, a series of previous studies were focused on optimizing the effects of hull deformation by securing a proper level of propulsion shaft stability and optimizing the relative inclination angle and oil film retention based on a quasi-static state, that is, Rules for the Classification of Steel Ships and experiences of shipyards. However, despite our efforts to resolve this issue, marine accidents involving stern tube bearing damage have continued to occur under relatively unattended ship motions in a transient state, that is, a quasi-static state that can possibly cause sudden stern flow field changes. Therefore, to improve the stability of the propulsion shaft, it is necessary to understand ship motions and the conditions of shafting systems in a dynamic state and a transient state when the system is designed. From this point of view, this study investigated the effect of changes in eccentric propeller forces on the motion of the propeller shaft in a representative transient state of a 50,000 deadweight tonnage tanker by means of the strain gauge method and a displacement sensor. The research findings demonstrate that propeller thrust fluctuations have a direct effect on the shaft stability by significant changes in the shaft motion that can lead to unbalanced supporting loads on the stern tube bearing. These results clearly reproduce the cause of damage to the ship in which the accident occurred at a reliable level and will be a reference for establishing pragmatic guidelines for preventing damage to similar ships in the future.
Can Biochar Improve the Sustainability of Animal Production?
Authors: Graves Carly , Kolar Praveen , Shah Sanjay , Grimes Jesse , Sharara Mahmoud
Published: May 2022

Animal production is a significant contributor of organic and inorganic contaminants in air, soil, and water systems. These pollutants are present beginning in animal houses and impacts continue through manure storage, treatment, and land application. As the industry is expected to expand, there is still a lack of affordable, sustainable solutions to many environmental concerns in animal production. Biochar is a low-cost, sustainable biomaterial with many environmental remediation applications. Its physicochemical properties have been proven to provide environmental benefits via the adsorption of organic and inorganic contaminants, promote plant growth, improve soil quality, and provide a form of carbon sequestration. For these reasons, biochar has been researched regarding biochar production, and application methods to biological systems have a significant influence on the moisture content, pH, microbial communities, and carbon and nitrogen retention. There remain unanswered questions about how we can manipulate biochar via physical and chemical activation methods to enhance the performance for specific applications. This review article addresses the positive and negative impacts of biochar addition at various stages in animal production from feed intake to manure land application.
Efficacy and Safety of Hybrid Cardiac Telerehabilitation in Patients with Hypertrophic Cardiomyopathy without Left Ventricular Outflow Tract Obstruction and Preserved Ejection Fraction—A Study Design
Authors: Sadowski Krzysztof , Piotrowicz Ryszard , Kłopotowski Mariusz , Wolszakiewicz Jadwiga , Lech Agnieszka , Witkowski Adam , Smolis-Bąk Edyta , Kowalik Ilona , Mierzyńska Anna , Piotrowska Dorota , Dobrowolski Piotr , Dąbrowski Maciej , Sadowy Ewa , Piotrowicz Ewa
Published: May 2022

Hypertrophic cardiomyopathy (HCM) is the most common congenital disease increasing the risk of sudden cardiac death. For many years, HCM patients were excluded from exercise training. However, there are data showing that patients with HCM undergoing supervised exercise training could improve physical performance without serious adverse events. A project was designed as a randomized clinical trial to assess the effectiveness and safety of hybrid cardiac rehabilitation (HCR)—a combination of hospital-based cardiac rehabilitation (1 month) with a new form of home-based telemonitored cardiac rehabilitation (2 months) in HCM patients without left ventricular (LV) outflow tract obstruction and preserved systolic function. Sixty patients who fulfil the inclusion criteria have been randomly assigned (1:1) to either HCR plus usual care (training group) or usual care only (control group). The primary endpoint is a functional capacity evaluated by peak oxygen uptake (pVO2). Secondary endpoints include workload time during the cardiopulmonary exercise testing, a six-minute walk test distance, NT-pro BNP level, echocardiographic parameters of the left ventricular diastolic function (E/A, E/e’, myocardial strain rate), right ventricular systolic pressure, a gradient in the LV outflow tract, and quality of life. The tertiary analysis includes safety, acceptance and adherence to the HCR program. Our research will provide innovative data on the effectiveness and safety of hybrid cardiac rehabilitation in HCM patients without LV outflow tract obstruction and preserved systolic function. Clinical trials registry: ClinicalTrials.gov Identifier NCT03178357.
Influence of Broadleaved Wood Conditioning by Pulsed Electric Field on Its Combustion Heat Characteristics
Authors: Popardowski Ernest , Kiełbasa Paweł
Published: May 2022

This publication presents changes in sawdust of selected deciduous trees as a consequence of impulse electric field (PEF) stimulation. The analyzed changes concerned the time–temperature characteristics created during the measurement of the heat of combustion of the audited material. Based on experience from previous studies, two alternatives of electric field strength and one variant of capacitor discharges (pulses) were adopted. The results were compared with the sample not treated with PEF. The selected parameters were the result of previous studies, in which the applied variants seemed to be the most promising, i.e., they gave the most diverse results. The research presented in this work has shown that the pulsed electric field affects the time and temperature characteristics of biological material. The changes are most pronounced for the last period of the combustion process, from the moment the maximum temperature was reached to the end of the process. The obtained results indicate that birch and ash react to PEF conditioning in a similar manner. The second group, due to the similarity of the obtained results, is oak and linden. It seems that, apart from the electric field strength, the obtained results are also influenced by the cellulose content in the tested wood. The described process has a very low energy-efficiency, but the reduction of the energy needed to generate the impulse could lead to the possibility of applying the obtained results in industry.
Double Linear Transformer for Background Music Generation from Videos
Authors: Yang Xueting , Yu Ying , Wu Xiaoyu
Published: May 2022

Many music generation research works have achieved effective performance, while rarely combining music with given videos. We propose a model with two linear Transformers to generate background music according to a given video. To enhance the melodic quality of the generated music, we firstly input note-related and rhythm-related music features separately into each Transformer network. In particular, we pay attention to the connection and the independence of music features. Then, in order to generate the music that matches the given video, the current state-of-the-art cross-modal inference method is set up to establish the relationship between visual mode and sound mode. Subjective and objective experiment indicate that the generated background music matches the video well and is also melodious.
Experimental Method for Flow Calibration of the Aircraft Liquid Cooling System
Authors: Zhao Yingjie , Yang Fan , Ma Yijiang
Published: May 2022

In the process of aircraft operation, the flow calibration of aircraft liquid cooling system has always been one of the research hotspots in engineering. Based on the principle of the differential pressure method, a new experimental flow calibration method is proposed for the aircraft liquid cooling system in this paper. In the reducer and the square bend of the aircraft liquid cooling system, the pressure difference will be generated. The flowmeter is used to measure the flow of the coolant, and the flow rate coefficient of the aircraft liquid cooling system can be calibrated. The experimental platform is established to conduct the flow calibration of the aircraft liquid cooling system, and the influence of the temperature and imported pressure on the flow will be investigated. Results indicate that the experimental method proposed is very effective, and the flow calibration can be realized without damaging the aircraft liquid cooling system.
Research and Application of a Prefabricated Spatial Reticulated Shell Support System for Large Cross-Section Tunnel in a Complex Urban Environment
Authors: Huang Mingli , Song Yuan , Zhang Xudong
Published: May 2022

To solve the technical problems of slow construction progress, low mechanization and high risk of shallow buried large cross-section tunnels in a complex urban environment, a series of spatial reticulated shell (SRS) support structures are developed in this paper. Moreover, the equipment of a multifunctional operation trolley is developed to install the SRS arch, and the construction technology system of the prefabricated SRS structure is proposed for a large cross-section tunnel. Therefore, the deformation characteristics of the end-plate joint component and jointless component are clarified by laboratory experiments. The construction mechanics’ simulation of the SRS arch is performed to obtain the tunnel deformation and structure stress based on the tunnel project of Panyu Square Station of Guangzhou Metro. A field application of the prefabricated SRS arch is carried out to realize the mechanized construction operation. The obtained results reveal that the end-plate joint component has better ductility, and its ultimate bearing capacity is basically consistent with the jointless component. The SRS arch can effectively control the deformation of the surrounding rock, improve the stress state of the structure, and reduce the plastic zone of shotcrete by numerical simulations. The overall stress of the SRS arch by field measurement represents the characteristics of “bigger on the upside and smaller on the downside” and “uneven symmetry”. Additionally, the successful application of the prefabricated SRS arch provides a scientific reference for mechanized construction of large cross-section tunnels.
Experimental Study on the Dust-Cleaning Performance of New Structure Microporous Membrane Filter Plate
Authors: Chen Lumin , Liu Zhe , Sun Yi , Qian Fuping , Han Yunlong , Lu Jinli
Published: May 2022

On the basis of the existing dust collector structure, this study designed a fan-shaped new structure microporous membrane filter plate (NSMMFP). The pressure distribution law of the NSMMFP can be obtained by measuring the wall surface peak pressure under different injection pressures. The powder attachment experiment was carried out to explore the influence of the dust moisture content on the dust stripping rate (DSR), and a high-speed camera was used to observe the peeling process of the dust. The results show that the peak pressure of each measuring point and the average wall surface peak pressure gradually increase with the injection pressure. The dust stripping quality (DSQ) and rate show an increasing trend as a whole as the injection distance. The DSR of the filter plate shows a downward trend when the dust quality G increases, while DSQ shows the opposite trend. Furthermore, as the dust moisture content increases, the DSQ and DSR gradually decrease. As the dust moisture content increases, the dust attached to the surface of the filter plate is more fragmented and peels from the surface of the filter plate during the dust cleaning process.
Optimization of the Efficient Extraction of Organic Components in Atmospheric Particulate Matter by Accelerated Solvent Extraction Technique and Its Application
Authors: Zhang Hao , Ren Yanqin , Wei Jie , Ji Yuanyuan , Bai Xurong , Shao Yanqiu , Li Hong , Gao Rui , Wu Zhenhai , Peng Zhijian , Xue Feng
Published: May 2022

Organic components in atmospheric fine particulate matter have attracted much attention and several scientific studies have been performed, although most of the sample extraction methods are time consuming and laborious. Accelerated solvent extraction (ASE) is a new sample extraction method offering number of advantages, such as low extraction cost, reduced solvent and time consumption, and simplified extraction protocols. In order to optimize ASE methods to determine the concentrations of organic compounds in atmospheric fine particulate matter, different parameters were set out for the experiment, and the optimal method was selected according to the recoveries of the standard (i.e., n−alkanes and polycyclic aromatic hydrocarbons (PAHs)). This study also involves a comparison of the optimal method with the traditional method of ultrasonic extraction (USE). In addition, the optimized method was applied to measure the mass concentrations of organic compounds (n−alkanes and PAHs) in fine particulate matter samples collected in Beijing. The findings showed that the average recovery of target compounds using ASE was 96%, with the majority of compounds falling within the confidence levels, and the ASE recoveries and precision were consistent with the USE method tested. Furthermore, ASE combines the advantages of high extraction efficiency, automation, and reduced solvent use. In conclusion, the optimal ASE methods can be used to extract organic components in atmospheric particulate matter and serve as a point of reference for the development of analytical methodologies for assessing organic compounds in atmospheric particulate matter in China.