bluuebunny/crossref_metadata_2025 huggingface dataset:
Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro
Authors: Zhao Meng-Yun , Liu Pan , Sun Chen , Pei Li-Jian , Huang Yu-Guang
Published: April 2022

Introduction: Cervical cancer is common in women. The present standardized therapies including surgery, chemotherapy, and radiotherapy are still not enough for treatment. Propofol is the most commonly used intravenous anesthetic agent for induction and maintenance of anesthesia and has been shown to exert anti-malignancy effects on cancer cells, inducing oxidative stress and apoptosis. However, the biological effects of propofol have not yet been systematically assessed. In this study, we examined the ferroptosis-related changes caused by propofol and the chemotherapeutic agent paclitaxel besides apoptosis in vitro.

Methods: Cervical cancer cell lines (C-33A and HeLa) were treated with propofol alone (1, 2, 5, 10, and 20 μg/ml) or in combination with paclitaxel (0.5, 1, and 5 μg/ml). The viability was assessed using cell counting kit-8 (CCK8), apoptosis was detected by flow cytometry, morphological changes of mitochondria were examined using transmission electron microscope (TEM), cellular reactive oxygen species (ROS), and intracellular ferrous ions were determined by fluorescence microscope or confocal microscopy. The expression and cellular localization of apoptosis and ferroptosis-related molecules were detected by Western blot and multiplex immunohistochemistry (mIHC), respectively. Calcusyn software was used to determine whether propofol has a synergistic effect with paclitaxel.

Results: Propofol and paclitaxel inhibited C-33A and HeLa cell viability. There were also synergistic effects when propofol and paclitaxel were used in combination at certain concentrations. In addition, propofol promoted paclitaxel-induced cervical cancer cell death via apoptosis. ROS level and Fe2+ concentrations were also influenced by different drug treatments. Furthermore, propofol, propofol injectable emulsion, and paclitaxel induced ferroptosis-related morphological changes of mitochondria in C-33A and HeLa cells. Ferroptosis-related signaling pathways including SLC7A11/GPX4, ubiquinol/CoQ10/FSP1, and YAP/ACSL4/TFRC were found to be changed under drug treatments.

Conclusion: Propofol showed synergistic anticancer effects with paclitaxel in cervical cancer cells. Propofol and paclitaxel may induce ferroptosis of cervical cancer cells besides apoptosis.
Antitumor Effect of Simvastatin in Combination With DNA Methyltransferase Inhibitor on Gastric Cancer via GSDME-Mediated Pyroptosis
Authors: Xia Ying , Jin Yong , Cui Daxiang , Wu Xia , Song Cunfeng , Jin Weilin , Huang Hai
Published: April 2022

Gasdermin E (GSDME) is one of the executors of pyroptosis, a type of programmed lytic cell death, which can be triggered by caspase-3 activation upon stimulation. Silenced GSDME expression due to promoter hypermethylation is associated with gastric cancer (GC), which is confirmed in the present study by bioinformatics analysis and methylation-specific PCR (MSP) test of GC cell lines and clinical samples. GC cell lines and mouse xenograft models were used to investigate the pyroptosis-inducing effect of the common cholesterol-depleting, drug simvastatin (SIM), allied with upregulating GSDME expression by doxycycline (DOX)- inducible Tet-on system or DNA methyltransferase inhibitor 5-Aza-2′-deoxycytidine (5-Aza-CdR). Cell viability assessment and xenograft tumour growth demonstrated that the tumour inhibition effects of SIM can be enhanced by elevated GSDME expression. Morphological examinations and assays measuring lactate dehydrogenase (LDH) release and caspase-3/GSDME protein cleavage underlined the stimulation of pyroptosis as an important mechanism. Using short hairpin RNA (shRNA) knockdown of caspase-3 or GSDME, and caspase-3-specific inhibitors, we provided evidence of the requirement of caspase-3/GSDME in the pyroptosis process triggered by SIM. We conclude that reactivating GSDME expression and thereby inducing cancer cell-specific pyroptosis could be a potential therapeutic strategy against GC.
Dynamics of Erythroferrone Response to Erythropoietin in Rats
Authors: Xu Peng , Wong Raymond S. M. , Krzyzanski Wojciech , Yan Xiaoyu
Published: April 2022

Background: Erythroferrone (ERFE) is a hormone identified recently as a master regulator connecting iron homeostasis and erythropoiesis. Serum ERFE concentrations significantly increase in animals and humans with normal or impaired kidney function after receiving exogenous erythropoiesis-stimulating agents (ESAs), which suggests it might be a predictive factor for erythropoiesis. To evaluate whether ERFE is an early, sensitive biomarker for long-term erythropoietic effects of ESAs, we investigated the relationship between ERFE dynamics and time courses of major erythropoietic responses to ESA treatment.

Methods: Healthy rats received single dose and multiple doses (thrice a week for 2 weeks) of recombinant human erythropoietin (rHuEPO) at three dose levels (100, 450, and 1350 IU/kg) intravenously. The rHuEPO and ERFE concentrations in plasma were determined at a series of time points after dosing. Erythropoietic effects including red blood cell count and hemoglobin concentrations were continuously monitored for 24 days (single dose) or 60 days (multiple doses). The expansion of erythroblasts in bone marrow was quantified by flow cytometry analysis.

Results: ERFE significantly increased within a few hours and return to baseline at 24 h after rHuEPO treatment. The ERFE response was enhanced after repeated treatment, which was consistent with the observed expansion of erythroblasts in the bone marrow. In addition, the dynamics of ERFE showed double peaks at approximately 2 and 10 h after rHuEPO stimulation, and the ERFE baseline displayed a significant circadian rhythm. There was a strong positive correlation between peak values of short-term ERFE responses and the long-term hemoglobin responses.

Conclusion: The stimulated release of ERFE is a rapid process within 24 h. The second peak in the ERFE response to rHuEPO suggests the presence of a feedback mechanism counterregulating the ESA stimulation. The early increase of ERFE at 2 h appears to be a predictor of the hemoglobin response at 14 days after single dose of rHuEPO. Under multiple-dose regimen, the enhanced ERFE responses still correlate with the peak hemoglobin responses. The ERFE baseline also exhibits a circadian rhythm.
Evaluation of Potential Herb-Drug Interactions Between Shengmai Injection and Losartan Potassium in Rat and In Vitro
Authors: Niu Zhenchao , Qiang Tingting , Lin Wenyong , Li Yiping , Wang Keyan , Wang Dan , Wang Xiaolong
Published: April 2022

Aim: The present study aimed to explore the potential herb-drug interactions (HDI) between Shengmai injection (SMI) and losartan potassium (LOS) based on the expression profiles of cytochromes P450 (CYP450) and drug transporters in rat and in vitro.

Methods: Different concentrations of SMI were used to explore the influence of SMI on the antihypertensive efficacy of LOS in the hypertension rat model established by N (omega)-nitro-L-arginine methyl ester (L-NAME) for 4 weeks. Subsequently, the serum concentration levels of LOS and losartan carboxylic acid (EXP3174) were determined by Liquid Chromatography Mass Spectrometry (LC-MS) and pharmacokinetic analysis. Human liver microsomes, human multidrug resistance protein 1 (MDR1/P-gp), and breast cancer resistance protein (BCRP) vesicles, human embryonic kidney 293 cell line with stable expression of the organic anion transporting polypeptide 1B1 (HEK293-OATP1B1 cells) and mock-transfected HEK293 (HEK293-MOCK) cells were used to verify the effects of SMI on CYP450 enzymes and drug transporters in vitro.

Results: Low, medium, and high concentrations of SMI increased the antihypertensive efficacy of LOS to varying degrees. The high dose SMI increased the half-life (t1/2), the maximum plasma concentration (Cmax), the area under the plasma concentration-time curve (AUC) from time zero to the time of the last measurable plasma concentration (AUC0-t), AUC from time zero to infinity (AUC0-∞), and mean residence time (MRT) values of LOS and decreased its apparent volume of distribution (Vd) and clearance (CL) values. The AUC0-t, AUC0-∞, and MRT of LOS were increased, whereas the CL was decreased by the medium concentration of SMI. In addition, the high, medium, and low doses of SMI increased the relative bioavailability (Frel) of LOS. SMI exhibited no significant effects on the pharmacokinetics of EXP3174. In vitro, SMI exhibited different suppressive effects on the enzyme activity levels of CYP1A2 (6.12%), CYP2B6 (2.72%), CYP2C9 (14.31%), CYP2C19 (12.96%), CYP2D6 (12.26%), CYP3A4 (3.72%), CYP2C8 (10.00–30.00%), MDR1 (0.75%), OATP1B1(2.03%), and BCRP (0.15%).

Conclusion: In conclusion, SMI improved the antihypertensive efficacy of LOS in the L-NAME-induced hypertension rat model by increasing the concentration of LOS, while leaving the concentration of EXP3174 intact. SMI affected the pharmacokinetic properties of LOS by decreasing the elimination of LOS. These effects might partly be attributed to the inhibition of the activities of CYP3A4, CYP2C9, and of the drug transporters (P-gp, BCRP, and OATP1B1) by SMI, which need further scrutiny.
Single Cell RNA-Seq Analysis of Human Red Cells
Authors: Jain Vaibhav , Yang Wen-Hsuan , Wu Jianli , Roback John D. , Gregory Simon G. , Chi Jen-Tsan
Published: April 2022

Human red blood cells (RBCs), or erythrocytes, are the most abundant blood cells responsible for gas exchange. RBC diseases affect hundreds of millions of people and impose enormous financial and personal burdens. One well-recognized, but poorly understood feature of RBC populations within the same individual are their phenotypic heterogeneity. The granular characterization of phenotypic RBC variation in normative and disease states may allow us to identify the genetic determinants of red cell diseases and reveal novel therapeutic approaches for their treatment. Previously, we discovered diverse RNA transcripts in RBCs that has allowed us to dissect the phenotypic heterogeneity and malaria resistance of sickle red cells. However, these analyses failed to capture the heterogeneity found in RBC sub-populations. To overcome this limitation, we have performed single cell RNA-Seq to analyze the transcriptional heterogeneity of RBCs from three adult healthy donors which have been stored in the blood bank conditions and assayed at day 1 and day 15. The expression pattern clearly separated RBCs into seven distinct clusters that include one RBC cluster that expresses HBG2 and a small population of RBCs that express fetal hemoglobin (HbF) that we annotated as F cells. Almost all HBG2-expessing cells also express HBB, suggesting bi-allelic expression in single RBC from the HBG2/HBB loci, and we annotated another cluster as reticulocytes based on canonical gene expression. Additional RBC clusters were also annotated based on the enriched expression of NIX, ACVR2B and HEMGN, previously shown to be involved in erythropoiesis. Finally, we found the storage of RBC was associated with an increase in the ACVR2B and F-cell clusters. Collectively, these data indicate the power of single RBC RNA-Seq to capture and discover known and unexpected heterogeneity of RBC population.
Periodontitis Is Associated With Heart Failure: A Population-Based Study (NHANES III)
Authors: Yan Yan , Mao Min , Li Yan-Qin , Chen Yong-Ji , Yu He-Dong , Xie Wen-Zhong , Huang Qiao , Leng Wei-Dong , Xiong Jie
Published: April 2022

Objectives: The aim of this study was to investigate the relationship between periodontitis and heart failure using the Third National Health and Nutrition Examination Survey (NHANES III).

Methods: Participants who had received a periodontal examination were included and investigated for the occurrence of heart failure. The included participants were divided into no/mild periodontitis and moderate/severe periodontitis groups according to their periodontal status. Weighted prevalence of heart failure was calculated, and weighted logistic regressions models were used to explore the association between periodontitis and heart failure. Possible influencing factors were then explored through subgroup analysis.

Results: Compared with that of the no/mild periodontitis group, the incidence of heart failure in participants with moderate/severe periodontitis was 5.72 times higher (95% CI: 3.76–8.72, p < 0.001). After adjusting for gender, age, race, body mass index, poverty income ratio, education, marital status, smoking status, drinking status, hypertension, diabetes, stroke, and asthma, the results showed that the incidence of heart failure in the moderate/severe group was 3.03 times higher (95% CI: 1.29–7.13, p = 0.012). Subgroup analysis showed that criteria, namely, male, 40–60 years old, non-Hispanic white, body mass index >30, poverty income ratio ≥1, not more than 12 years of education, currently drinking, stroke but no diabetes, or asthma supported moderate/severe periodontitis as a risk factor for heart failure (p < 0.05).

Conclusion: According to data from this nationally representative sample from the United States, periodontitis is associated with an increased risk of heart failure.
Measuring Perceived Research Competence of Junior Researchers
Authors: Marrs Sarah A. , Quesada-Pallarès Carla , Nicolai Korinthia D. , Severson-Irby Elizabeth A. , Martínez-Fernández J. Reinaldo
Published: April 2022

Graduates of doctoral (Ph.D.) programs are expected to be competent at designing and conducting research independently. Given the level of research competence needed to successfully conduct research, it is important that assessors of doctoral programs (e.g., faculty and staff) have a reliable and validated tool for measuring and tracking perceived research competence among their students and graduates. A high level of research competence is expected for all Ph.D. graduates worldwide, in addition to in all disciplines/fields. Moreover, graduates of Ph.D. programs may complete their studies in one country but then obtain a research position in another country, emphasizing the need to ensure that all doctoral programs are fostering similar levels of research competence. Thus, the purpose of this study was to gather additional evidence for validity and reliability of the Research Competence (R-Comp) scale. Specifically, we sought to extend the findings of by adapting the scale, translating it to other languages, and applying the tool with a sample of early stage researchers. Our findings provide initial evidence that the adapted PR-Comp is appropriate for use in three languages and across a variety of disciplines/programs of study.
The Magnetic Urtext: Restoration as Music Interpretation
Authors: Canazza Sergio , Schubert Emery , Chmiel Anthony , Pretto Niccolò , Rodà Antonio
Published: April 2022

This paper discusses historical-critical thought to address the problems of restoration and preservation of tape music, proposing viable solutions to the matter of digitizing the historically valuable data that exists on and is represented by magnetic tapes. A detailed program of research and restoration and some software for helping in creation of critical editions of the musical works are proposed. We also present some of the issues and controversies that must be considered and approaches we have applied in the preservation of tape music, highlighting how these interpretations can impact later performances (playback) of these tape documents. Fundamentally, we argue that the act of tape music restoration has a parallel with the interpretation of the “Urtext score” in the performance of music from the Common Practice Era.
Combined’ Neck/Back Pain and Psychological Distress/Morbidity Among the Saudi Population: A Cross-Sectional Study
Authors: Al-Ghamdi Sameer , Shubair Mamdouh M. , Angawi Khadijah , Al-Zahrani Jamaan , Khormi Abdulrahman Ali M. , Alshammari Reem Falah , Alshammari Nawaf Safaq , Aldahash Raed , Otayf Bander Yahya , Al-Zahrani Hayat Saleh , Aleshaiwi Manayir Sultan , Aldossari Khaled K.
Published: April 2022

Background

Psychological distress/morbidity is amongst the primary reason for the cause of pain at multiple sites, its progression, and recovery. Though still not very clear if physical pain in the neck or the back may predict psychological morbidities or not. Thus, we investigated the association between combined neck or back pain and psychological distress/morbidity.

Methods

A cross-sectional study was conducted in Al-Kharj, Saudi Arabia, including 1,003 individuals. The questionnaire comprised of General Health Questionnaire-12 (GHQ-12) and some questions about neck and back pain. Data analysis was done using statistical software SPSS version 26.0.

Results

The results of the multivariate analysis revealed a significant positive association between neck/back pain status and total GHQ score (unstandardized Beta = 2.442, P ≤ 0.0001). Having neck/back pain had almost a 2.5 times greater risk of psychological distress/morbidity. Further, females were more likely to have a higher risk of psychological distress/morbidity (unstandardized Beta = 1.334, P = 0.007) than males while adjusting for sociodemographic and clinical characteristics.

Conclusion

The combination of neck and back pain was significantly associated with the Saudi population’s psychological problems. Therefore, the Saudi government needs to devise high-risk strategies and allocate adequate resources to the cause so that at-risk people can be shielded from the adverse complications arising from this condition in the long run.
Eruptivity Criteria for Two-Dimensional Magnetic Flux Ropes in the Solar Corona
Authors: Rice Oliver E. K. , Yeates Anthony R.
Published: April 2022

We apply the magneto-frictional approach to investigate which quantity or quantities can best predict the loss of equilibrium of a translationally-invariant magnetic flux rope. The flux rope is produced self-consistently by flux cancellation combined with gradual footpoint shearing of a coronal arcade which is open at the outer boundary. This models the magnetic field in decaying active regions on the Sun. Such a model permits two types of eruption: episodic small events caused by shearing and relaxation of the overlying arcade, and major eruptions of the main low-lying coronal flux rope. Through a parameter study, we find that the major eruptions are best predicted not by individual quantities but by thresholds in the ratios of squared rope current to either magnetic energy or relative magnetic helicity. We show how to appropriately define the latter quantity for translationally-invariant magnetic fields, along with a related eruptivity index that has recently been introduced for three-dimensional magnetic fields. In contrast to previous configurations studied, we find that the eruptivity index has only a weak predictive skill, and in fact is lower prior to eruption, rather than higher. This is because the overlying background magnetic field has the same direction as the arcade itself. Thus we propose that there are a whole class of solar eruptions that cannot be predicted by a high eruptivity index.
Safeguarding Athletes Against Head Injuries Through Advances in Technology: A Scoping Review of the Uses of Machine Learning in the Management of Sports-Related Concussion
Authors: Tjønndal Anne , Røsten Stian
Published: April 2022

Sports injury prevention is an important part of the athlete welfare and safeguarding research field. In sports injury prevention, sport-related concussion (SRC) has proved to be one of the most difficult and complex injuries to manage in terms of prevention, diagnosis, classification, treatment and rehabilitation. SRC can cause long-term health issues and is a commonly reported injury in both adult and youth athletes around the world. Despite increased knowledge of the prevalence of SRC, very few tools are available for diagnosing SRC in athletic settings. Recent technological innovations have resulted in different machine learning and deep learning methodologies being tested to improve the management of this complex sports injury. The purpose of this article is to summarize and map the existing research literature on the use of machine learning in the management of SRC, ascertain where there are gaps in the existing research and identify recommendations for future research. This is explored through a scoping review. A systematic search in the three electronic databases SPORTDiscus, PubMed and Scopus identified an initial 522 studies, of which 24 were included in the final review, the majority of which focused on machine learning for the prediction and prevention of SRC (N = 10), or machine learning for the diagnosis and classification of SRC (N = 11). Only 3 studies explored machine learning approaches for the treatment and rehabilitation of SRC. A main finding is that current research highlights promising practical uses (e.g., more accurate and rapid injury assessment or return-to-sport participation criteria) of machine learning in the management of SRC. The review also revealed a narrow research focus in the existing literature. As current research is primarily conducted on male adolescents or adults from team sports in North America there is an urgent need to include wider demographics in more diverse samples and sports contexts in the machine learning algorithms. If research datasets continue to be based on narrow samples of athletes, the development of any new diagnostic and predictive tools for SRC emerging from this research will be at risk. Today, these risks appear to mainly affect the health and safety of female athletes.
Toxicological Assessment of Flavor Ingredients in E-Vapor Products
Authors: Sciuscio Davide , Calvino-Martin Florian , Kumar Ashutosh , Langston Timothy B. , Martin Elyette , Marescotti Diego , Mathis Carole , Hoeng Julia , Peitsch Manuel C. , Smith Donna C. , Gogova Maria , Vanscheeuwijck Patrick , Lee Kyeonghee M.
Published: April 2022

Many flavor ingredients are often used in potentially reduced-risk tobacco products (such as e-vapor products). Although most are “generally recognized as safe (GRAS)” when used in food, there is limited information available on their long-term health effects when delivered by inhalation. While obtaining route-of-exposure-specific toxicological data on flavor ingredients is critical to product evaluation, the large number of individual flavor ingredients available and their potential combinations render classical toxicological assessment approaches impractical, as they may require years of preclinical investigations and thousands of laboratory animals. Therefore, we propose a pragmatic approach in which flavor ingredients are initially assigned to groups of structurally related compounds (Flavor Groups), from which flavor group representatives (FGR) are then selected and tested individually and as a mixture in vitro and in vivo. The premise is that structurally related compounds would have comparable metabolic and biological activity and that the data generated using FGRs could support the toxicological assessment of other structurally related flavor ingredients of their respective Flavor Groups. This approach is explained in a step-wise manner and exemplified by a case study, along with its strengths, limitations as well as recommendations for further confirmatory testing. Once completed, this FGR approach could significantly reduce the time and resources required for filling the data gap in understanding the health risks of many flavor ingredients while also minimizing the need for laboratory animals.
Diagnostic Value of Six Tumor Markers for Malignant Pleural Effusion in 1,230 Patients: A Single-Center Retrospective Study
Authors: Fan Xin , Liu Yanqing , Liang Zhigang , Wang Shanshan , Yang Jing , Wu Aihua
Published: April 2022

Background:The diagnostic value of tumor markers in pleural effusion (PE) and serum for malignant pleural effusion (MPE) is still in debate. This study aimed to evaluate the diagnostic value of six tumor markers in PE, serum, and the corresponding PE/serum (PE/S) ratio in distinguishing MPE from benign pleural effusion (BPE).

Methods:A total of 1,230 patients with PE (452 MPEs and 778 BPEs) were retrospectively included in the study. PE and serum levels of carcinoembryonic antigen (CEA), carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 125 (CA125), carbohydrate antigen 19-9 (CA19-9), cytokeratin 19 fragment (CYFRA 21-1), and neuron-specific enolase (NSE) were measured. The area under the curve (AUC) was used to assess the single and combined diagnostic values of the six tumor markers for MPE.

Results:The levels of the six tumor markers in PE, serum, and PE/S were significantly higher in MPE than that in BPE, except for serum CA125. PE CEA showed the highest AUC [0.890 (0.871–0.907)] at a cut-off value of 3.7 ng/ml compared to any single tumor marker using receiver operating characteristic (ROC) analysis. The specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), and negative likelihood ratio (NLR) of PE CEA were 74.1%, 95.5%, 90.5%, 86.4%, 16.47, and 0.27, respectively. The combination of PE CEA and serum CYFRA21-1 showed the best diagnostic performance with an AUC of 0.934 (sensitivity, 79.9%; specificity, 95.7%, PPV, 90.5; PLR, 17.35) among all two or three combinations. Besides, serum CYFRA21-1 was the best diagnostic tumor marker in distinguishing cytology-negative MPE from BPE at a cut-off value of 3.0 ng/ml.

Conclusion:PE CEA was the best diagnostic tumor marker in distinguishing MPE from BPE. Serum CYFRA21-1 was the best diagnostic tumor marker in distinguishing cytology-negative MPE from BPE. The combination of PE CEA and serum CYFRA21-1 could increase the diagnostic performance in distinguishing MPE from BPE and cytology-negative MPE from BPE.
Improving Winter Wheat Yield Forecasting Based on Multi-Source Data and Machine Learning
Authors: Sun Yuexia , Zhang Shuai , Tao Fulu , Aboelenein Rashad , Amer Alia
Published: April 2022

To meet the challenges of climate change, population growth, and an increasing food demand, an accurate, timely and dynamic yield estimation of regional and global crop yield is critical to food trade and policy-making. In this study, a machine learning method (Random Forest, RF) was used to estimate winter wheat yield in China from 2014 to 2018 by integrating satellite data, climate data, and geographic information. The results show that the yield estimation accuracy of RF is higher than that of the multiple linear regression method. The yield estimation accuracy can be significantly improved by using climate data and geographic information. According to the model results, the estimation accuracy of winter wheat yield increases dramatically and then flattens out over months; it approached the maximum in March, with R2 and RMSE reaching 0.87 and 488.59 kg/ha, respectively; this model can achieve a better yield forecasting at a large scale two months in advance.
Effects of Exogenous Brassinolide Application at the Silking Stage on Nutrient Accumulation, Translocation and Remobilization of Waxy Maize under Post-Silking Heat Stress
Authors: Xu Yueming , Zhang Xiaoyu , Yang Huan , Lu Dalei
Published: April 2022

Exogenous brassinolide (BR) application is a feasible measure to alleviate abiotic stresses on crop productivity. The effects of BR application at the silking-stage on the accumulation, translocation, and remobilization of dry matter (DM) and nutrients (nitrogen, phosphorus, and potassium) of waxy maize exposed to post-silking high temperature (HT) were studied using Jingkenuo2000 (JKN2000, heat-tolerant) and Yunuo7 (YN7, heat-sensitive) as materials. BR application mitigated the penalty of HT on grain yield. HT reduced the post-silking accumulation and increased the translocation of pre-silking DM and nutrients in YN7. In JKN2000, accumulation and remobilization of DM were unaffected by HT. The contribution rate of DM and nutrients translocation to grain yield were unaffected by HT in JKN2000 and increased in YN7. Under HT, the accumulation, translocation, and remobilization of DM were unaffected by BR application, whereas the nitrogen, phosphorus, and potassium response were dependent on hybrids. The harvest index of DM and nutrients in response to HT and BR were different between the two hybrids. In conclusion, BR application relieved the negative effects of HT mainly caused by the increased post-silking accumulation and remobilization of DM and nitrogen, and the alleviation was more obvious in the heat-tolerant hybrid.
Effect of Organic and Conventional Production on the Quality of Lemon “Fino 49”
Authors: Sánchez-Bravo Paola , Noguera-Artiaga Luis , Martínez-Tomé Juan , Hernández Francisca , Sendra Esther
Published: April 2022

Since the end of the 20th century, organic foods have gained interest within the world population. The lemon is a fruit that is acquiring great prominence in the markets. Its use is based on its appreciated aroma and its content of bioactive compounds, but these attributes are greatly influenced by agronomic practices. To study the influence that organic farming has on the quality of “Fino 49” lemon variety in Spain, this citrus fruit has been characterized based on its morphological, functional, aromatic, and sensory properties. The results showed that conventional farming led to larger and elongated fruits (121.75 g of fruit weight and 58.35 and 79.66 mm of equatorial and polar diameter, respectively), with a higher lightness (L*) in lemon skin (73.38) and higher content of organic acids (malic, citric, and succinic) and sugars (glucose and fructose). On the other hand, organic farming had a higher content of volatile compounds in lemon juice (2537 mg L−1), especially limonene (increase of ~20%), which is related to the greater odor and flavor found in the organic samples by consumers (4.8 and 5.2, respectively). Furthermore, organic lemons had a better acceptance rate by consumers.
Impacts of Air Velocity Treatments under Summer Conditions: Part II—Heavy Broiler’s Behavioral Response
Authors: Akter Suraiya , Liu Yingying , Cheng Bin , Classen John , Oviedo Edgar , Harris Dan , Wang-Li Lingjuan
Published: April 2022

Broiler chickens exposed to heat stress adapt to various behavioral changes to regulate their comfortable body temperature, which is critical to ensure their performance and welfare. Hence, assessing various behavioral responses in birds when they are subjected to environmental changes can be essential for assessing their welfare under heat-stressed conditions. This study aimed to evaluate the effect of two air velocity (AV) treatments on heavy broilers’ behavioral changes from 43 to 54 days under summer conditions. Two AV treatments (high and low) were applied in six poultry growth chambers with three chambers per treatment and 44 COBB broilers per chamber from 28 to 61 days in the summer of 2019. Three video cameras placed inside each chamber (2.44 m × 2.44 m × 2.44 m in dimension) were used to record the behavior of different undisturbed birds, such as feeding, drinking, resting, standing, walking, panting, etc. The results indicate that the number of chickens feeding, drinking, standing, walking, sitting, wing flapping, and leg stretching changed under AV treatments. High AV increased the number of chickens feeding, standing, and walking. Moreover, a two-way interaction with age and the time of day can affect drinking and panting. This study provides insights into heavy broilers’ behavioral changes under heat-stressed conditions and AV treatments, which will help guide management practices to improve birds’ performance and welfare under commercial conditions in the future.
A Whole Genome Sequencing-Based Genome-Wide Association Study Reveals the Potential Associations of Teat Number in Qingping Pigs
Authors: Liu Zezhang , Li Hong , Zhong Zhuxia , Jiang Siwen
Published: April 2022

Teat number plays an important role in the reproductive performance of sows and the growth of piglets. However, the quantitative trait loci (QTLs) and candidate genes for the teat number-related traits in Qingping pigs remain unknown. In this study, we performed GWAS based on whole-genome single-nucleotide polymorphisms (SNPs) and insertions/deletions (Indels) for the total number of teats and five other related traits in 100 Qingping pigs. SNPs and Indels of all 100 pigs were genotyped using 10× whole genome resequencing. GWAS using General Linear Models (GLM) detected a total of 28 SNPs and 45 Indels as peak markers for these six traits. We also performed GWAS for the absolute difference between left and right teat number (ADIFF) using Fixed and random model Circulating Probability Unification (FarmCPU). The most strongly associated SNP and Indel with a distance of 562,788 bp were significantly associated with ADIFF in both GLM and FarmCPU models. In the 1-Mb regions of the most strongly associated SNP and Indel, there were five annotated genes, including TRIML1, TRIML2, ZFP42, FAT1 and MTNR1A. We also highlighted TBX3 as an interesting candidate gene for SSC14. Enrichment analysis of candidate genes suggested the Wnt signaling pathway may contribute to teat number-related traits. This study expanded significant marker-trait associations for teat number and provided useful molecular markers and candidate genes for teat number improvement in the breeding of sows.
Effects of Pelleting and Long-Term High-Temperature Stabilization on Vitamin Retention in Swine Feed
Authors: Wang Huakai , Li Longxian , Zhang Nan , Zhang Tuan , Ma Yongxi
Published: April 2022

The objective of this study was to study the effect of pelleting and long-term high-temperature stabilization on the retention of vitamin A, vitamin E, vitamin B2, and vitamin B6 in swine feed. Piglet diets (diet 1 and 3) were pelleted after conditioning at 83 °C for 120 s, and were high-temperature stabilized at 90 °C for 8.5 min after pelleting; the finishing pig diets (diet 2, 4, and 5) were pelleted after conditioning at 82 °C for 90 s, and were high-temperature stabilized at 85 °C for 9 min after pelleting; the samples were obtained before condition, after condition, after pelleting, and after cooling. The contents of vitamin A and vitamin E in diets 1–5 and vitamin B2, and vitamin B6 in diets 3–5 were detected. The results showed that: (1) the conditioning process had no significant effect on the retention of vitamin A, vitamin E, vitamin B2, and vitamin B6 in all experimental diets (p > 0.05); (2) the pelleting process and high-temperature stabilization process after pelleting had different degrees of influence on vitamins, among which the stabilization process had a more significant effect on the retention of vitamins. After pelleting and long-term high-temperature stabilization, the retention of vitamin A, vitamin E, and B2, and vitamin B6 were 68.8–77.3%, 56.9–90.1%, 63.8–70.3%, and 60.1–67.0%, respectively. In the process of pelleting and long-term high-temperature stabilization, the retention of vitamin A, vitamin E, vitamin B2, and vitamin B6 in the feed were significantly reduced (p < 0.05). Therefore, vitamin loss during high temperature and over a long period of time is worth considering, and vitamins must be over-supplemented.
Iron Effects on Clostridioides difficile Toxin Production and Antimicrobial Susceptibilities
Authors: Yamaki Jason , Chawla Swati , Tong Shirley , Lozada Kate Alison , Yang Sun
Published: April 2022

Despite the benefits of red blood cell (RBC) transfusion therapy, it can render patients vulnerable to iron overload. The excess iron deposits in various body tissues cause severe complications and organ damage such as cardiotoxicity and mold infections. Clostridioides difficile infection (CDI) is the most common cause of nosocomial diarrhea among cancer patients and is associated with significant morbidity and mortality. Our study aims to determine the role of iron overload and the effects of iron chelators on CDI. Our results demonstrated that iron (Fe3+) stimulated the growth of C. difficile with increased colony formation units (CFU) in a dose-dependent manner. Exposure to excess iron also increased the gene expression levels of tcdA and tcdB. The production of C. difficile toxin A, necessary for the pathogenesis of C. difficile, was also elevated after iron treatment. In the presence of excess iron, C. difficile becomes less susceptible to metronidazole with significantly elevated minimum inhibitory concentration (MIC) but remains susceptible to vancomycin. Iron-stimulated colony formation and production of C. difficile toxins were effectively diminished by iron chelator deferoxamine co-treatment. Incorporating iron overload status as a potential factor in developing a risk prediction model of CDI and antibiotic treatment response may aid clinical practitioners in optimizing CDI management in oncology patients.
Antimicrobial Prescribing Confidence and Knowledge Regarding Drug Resistance: Perception of Medical Students in Malaysia and the Implications
Authors: Haque Mainul , Ara Tasim , Haq Md. Ahsanul , Lugova Halyna , Dutta Siddhartha , Samad Nandeeta , Rabiu Abubakar Abdullahi , Syed Mohdhar Sharifah Shasha Binti , Rahman Md. Mahabubur , Islam Salequl , Adnan Nihad , Ahmad Rahnuma , Binti Abdullah Shahidah Leong , Ismail Mohd Hafizi Bin , Godman Brian
Published: April 2022

Background: Worldwide, microbes are becoming more challenging by acquiring virulent skills to adapt and develop antimicrobial resistance (AMR). This is a concern as AMR increases morbidity, mortality, and costs. Consequently, physicians need to be trained on appropriate antimicrobial prescribing, starting as medical students. Objective: To evaluate medical students’ confidence in antimicrobial prescribing and AMR. Methods: Cross-sectional study assessing medical students’ knowledge, perception, and confidence in prescribing antimicrobials and AMR in a Malaysian University. A universal sampling method was used. Results: Most responding students believed that educational input regarding overall prescribing was sufficient. Regarding the principle of appropriate and accurate prescriptions, female medical students had less knowledge (odds ratio (OR) = 0.51; 95% confidence interval (CI) 0.25–0.99; p = 0.050). Year-IV and Year-V medical students had more excellent knowledge than Year-III students regarding confidence in potential antibiotic prescribing once qualified. Year-V students also showed an appreciably higher confidence in the broad principles of prescribing, including antibiotics for infectious diseases, compared to those in other years. Conclusion: Overall, medical students gain more knowledge and confidence regarding the potential prescribing of antimicrobials as their academic careers progress. This is important given concerns with the current excessive use of antimicrobials in Malaysia.
Breaking the Rebellion: Photodynamic Inactivation against Erwinia amylovora Resistant to Streptomycin
Authors: Wimmer Annette , Glueck Michael , Ckurshumova Wenzi , Liu Jun , Fefer Michael , Plaetzer Kristjan
Published: April 2022

Global crop production depends on strategies to counteract the ever-increasing spread of plant pathogens. Antibiotics are often used for large-scale treatments. As a result, Erwinia amylovora, causal agent of the contagious fire blight disease, has already evolved resistance to streptomycin (Sm). Photodynamic Inactivation (PDI) of microorganisms has been introduced as innovative method for plant protection. The aim of this study is to demonstrate that E. amylovora resistant to Sm (E. amylovoraSmR) can be killed by PDI. Two photosensitizers, the synthetic B17-0024, and the natural derived anionic sodium magnesium chlorophyllin (Chl) with cell-wall-permeabilizing agents are compared in terms of their photo-killing efficiency in liquid culture with or without 100 µg/mL Sm. In vitro experiments were performed at photosensitizer concentrations of 1, 10 or 100 µM and 5 or 30 min incubation in the dark, followed by illumination at 395 nm (radiant exposure 26.6 J/cm2). The highest inactivation of seven log steps was achieved at 100 µM B17-0024 after 30 min incubation. Shorter incubation (5 min), likely to represent field conditions, reduced the photo-killing to 5 log steps. Chlorophyllin at 100 µM in combination with 1.2% polyaspartic acid (PASA) reduced the number of bacteria by 6 log steps. While PASA itself caused some light independent toxicity, an antibacterial effect (3 log reduction) was achieved only in combination with Chl, even at concentrations as low as 10 µM. Addition of 100 µg/mL Sm to media did not significantly increase the efficacy of the photodynamic treatment. This study proves principle that PDI can be used to treat plant diseases even if causative bacteria are resistant to conventional treatment. Therefore, PDI based on natural photosensitizers might represent an eco-friendly treatment strategy especially in organic farming.
Plasma Free Thiol Levels during Early Sepsis Predict Future Renal Function Decline
Authors: van der Slikke Elisabeth C. , Boekhoud Lisanne , Bourgonje Arno R. , Olgers Tycho J. , ter Maaten Jan C. , Henning Robert H. , van Goor Harry , Bouma Hjalmar R.
Published: April 2022

Sepsis is a life-threatening syndrome characterized by acute organ dysfunction due to infection. In particular, acute kidney injury (AKI) is common among patients with sepsis and is associated with increased mortality and morbidity. Oxidative stress is an important contributor to the pathogenesis of sepsis-related AKI. Plasma free thiols (R-SH) reflect systemic oxidative stress since they are readily oxidized by reactive species and thereby serve as antioxidants. Here, we aimed to assess the concentrations of serum free thiols in sepsis and associate these with major adverse kidney events (MAKE). Adult non-trauma patients who presented at the emergency department (ED) with a suspected infection were included. Free thiol levels and ischemia-modified albumin (IMA), a marker of oxidative stress, were measured in plasma at baseline, at the ward, and at three months, and one year after hospitalization. Plasma free thiol levels were lower at the ED visit and at the ward as compared to three months and one year after hospital admission (p < 0.01). On the contrary, plasma levels of IMA were higher at the ED and at the ward compared to three months and one year after hospital admission (p < 0.01). Furthermore, univariate logistic regression analyses showed that plasma free thiol levels at the ED were inversely associated with long-term renal function decline and survival at 90 days (MAKE90) and 365 days (MAKE365) (OR 0.43 per standard deviation [SD] [0.22–0.82, 95% CI], p = 0.011 and OR 0.58 per SD [0.34–0.96, 95% CI], p = 0.035, respectively). A multivariate regression analysis revealed an independent association of plasma free thiols at the ED (OR 0.52 per SD [0.29–0.93, 95% CI], p = 0.028) with MAKE365, even after adjustments for age, eGFR at the ED, SOFA score, and cardiovascular disease. These data indicate the clear role of oxidative stress in the pathogenesis of sepsis-AKI, as reflected in the lower plasma free thiol levels and increased levels of IMA.
Effect of Weld Pool Flow and Keyhole Formation on Weld Penetration in Laser-MIG Hybrid Welding within a Sensitive Laser Power Range
Authors: Jing Haohao , Ye Xin , Hou Xiaoqi , Qian Xiaoyan , Zhang Peilei , Yu Zhishui , Wu Di , Fu Kuijun
Published: April 2022

The weld penetration variation in laser-MIG hybrid welding under sensitive laser power range was investigated by welding experiments and CFD (computational fluid dynamics) simulation. During this investigation, joints of AH36 sheets were welded with varying laser powers by the laser-MIG hybrid welding process. In addition, the CFD model was established based on experimental parameters and measurement results. Moreover, surface tension, electromagnetic force, buoyancy, recoil pressure, evaporative condensation, evaporative heat exchange, melt drop transfer, and other factors were considered. The influence of various factors on molten pool depth and keyhole depth were studied, including temperature, velocity, and flow direction of liquid metal. The results show that the weld-forming effect is better at the laser power is 7.5 kW in the range of sensitive laser power. After the keyhole is formed, its depth gradually entered the stage of linear increase, oscillation increase, and oscillation balance. Increasing the laser power can effectively shorten the time of the two growth stages and allow the keyhole to enter the balance stage earlier. During the oscillation balance state of the keyhole, the molten metal under the keyhole flowed to the molten pool root in the reverse direction of welding; it can also promote weld penetration.
Changes of Physical and Mechanical Properties of Coral Reef Limestone under CO2–Seawater–Rock Interaction
Authors: Zhong Yu , Li Qi , Wang Ren , Yao Ting
Published: April 2022

Large amounts of anthropogenic CO2 in the atmosphere are taken up when the ocean alters the seawater carbonate system, which could have a significant impact on carbonate-rich sediments. Coral reef limestone is a special biogenic carbonate, which is mainly composed of calcium carbonate. When carbonate-rich rocks are brought into contact with a CO2 weak acid solution, they will be dissolved, which may affect the physical and mechanical properties of the rock. In this paper, the physical and chemical interactions between CO2, seawater and the framework structure reef limestone were studied based on an experiment conducted in a hydrothermal reactor. The solution was analyzed for dissolved Ca2+ concentration during the reaction, and the rock mass, effective volume (except for the volume of open pores), permeability, images from electron microscopy and X-ray microtomography were contrasted before and after immersion. The uniaxial compressive and tensile strength tests were conducted, respectively, to clarify the mechanical response of the rock after the reaction. The results indicate that dissolution occurred during the reaction, and the calcium ions of the solution were increased. The physical properties of the rock were changed, and the permeability significantly increased. Because the rocks were soaked for only 15 days, the total cumulative amount of calcium carbonate dissolved was less, and the mechanical properties were not affected.
The New Physics in LILITA_N21: An Improved Description of the Reaction 190 MeV 40Ar + 27Al
Authors: Di Nitto Antonio , Davide Federico , Vardaci Emanuele , Bianco Davide , La Rana Giovanni , Mercogliano Daniela
Published: April 2022

In this paper, light charged particle emission in the evaporation residue channel for the 190 MeV 40Ar + 27Al reaction leading to 67Ga composite nuclei at Ex = 91 MeV and angular momentum up to 46 ℏ has been re-analyzed. The main goal was to study the decay of 67Ga on the basis of an extended set of observables in order to provide a description of the evaporative decay cascades using the multistep Monte Carlo approach. The proton and α-particle energy spectra along with their angular distributions and ratios of differential multiplicities have been considered. The measured observables were compared with statistical model calculations. Having used a single-step Monte Carlo approach and standard parameters decades ago, the model does not provide a good description of the full dataset. Only a subset of the data was reproduced by assuming emitting nuclei with very large deformed shapes in a previous work published in the late 1980s. In the reported analysis, better agreement has been observed. Using the new transmission coefficients from the Optical Model, the parameters of which have recently been derived, the multi-step approach and the introduction of a nuclear shape description based on the nuclear stratosphere allowed us to realize a significant improvement.