bluuebunny/crossref_metadata_2025 huggingface dataset:
Peeling Away the Layers of Workplace Gossip: A Framework, Review, and Future Research Agenda to Study Workplace Gossip as a Dynamic and Complex Behavior
Authors: Begemann Vanessa , Lehmann-Willenbrock Nale , Stein Maie
Published: April 2023

Workplace gossip, a ubiquitous organizational behavior broadly defined as talking about an absent target in an evaluative way, has received increasing scientific attention over the past few years. The complexity and dynamism of the workplace gossip phenomenon create challenging research conditions such that studies focusing on the allegedly same type of workplace gossip can produce differential findings. To address this problem and better align theory and methodology, our manuscript first proposes a framework of workplace gossip that accounts for the interdependencies of the context-embeddedness and dynamism of workplace gossip. Guided by this framework, we systematically evaluate extant workplace gossip research, spanning a total of N = 104 empirical research articles. Highlighting current methodological challenges that indicate a neglect of the dynamic nuances and contexts of workplace gossip, we argue that previous organizational research painted an overly simplistic picture of workplace gossip. By looking beyond traditional organizational research designs, we derive recommendations to advance research on workplace gossip and, ultimately, to establish it as a complex and dynamic social interaction behavior.
Influence of Laser Treatment Medium on the Surface Topography Characteristics of Laser Surface-Modified Resorbable Mg3Zn Alloy and Mg3Zn1HA Nanocomposite
Authors: Bupesh Raja V. K. , Parande Gururaj , Kannan Sathish , Sonawwanay Puskaraj D. , Selvarani V. , Ramasubramanian S. , Ramachandran D. , Jeremiah Abishek , Akash Sundaraeswar K. , Satheeshwaran S. , Gupta Manoj
Published: April 2023

In this study, a Mg3Zn alloy and a Mg3Zn1HA nanocomposite were fabricated through disintegrated melt deposition, followed by the hot extrusion process. The specimens were subjected to fiber laser surface modification in air and demineralized water (DM) medium to determine the influence of the laser treatment medium on the microstructure, microhardness, grain size, and surface topography. During the laser treatment, the samples under the water medium produced a lower surface roughness when compared with the air medium. The atomic force microscope (AFM) surface topography scan showed the presence of surface modifications caused by the presence of nanohydroxyapatite (nHA) in the matrix, resulting in a 367.4% and 632.4% increase in the surface roughness (Ra) in the Mg3Zn1HA nanocomposite when compared with the Mg3Zn alloy in water and air media, respectively.
Microstructure, Phase Composition, and Mechanical Properties of a Layered Bimetallic Composite ER70S-6-ER309LSI Obtained by the WAAM Method
Authors: Kabaldin Yury , Shatagin Dmitrii , Ryabov Dmitrii , Solovyov Alexander , Kurkin Andrey
Published: April 2023

Currently, additive manufacturing technologies for materials and products are being implemented and improved. This is due to the possibility of creating workpieces with complex geometric shapes and specified functional gradient properties. The materials with the most complex functional properties demanded by the military–energy industry include bimetals of the “low-alloyed carbon steel—stainless chromium-nickel steel” type. One of the promising ways to obtain bimetallic products is the WAAM (Wire Arc Additive Manufacturing) technology. Despite the large scientific groundwork, the composition, structure, and properties of bimetallic composites produced by WAAM have not been sufficiently studied. The aim of the current work is to study the effect of WAAM parameters and the subsequent heat treatment on the composition, structure, and physical and mechanical properties of the bimetallic composite “ER70S-6-R309LSI”. Spectral, metallographic, and X-ray diffraction studies were carried out, as were mechanical tests of the samples obtained under various WAAM modes. In order to improve the composites’ properties, various types of heat treatments were applied. It is shown that the WAAM modes, the building strategy, and heat treatment determine the structure of layers and transition zones, as well as the mechanical characteristics of the composite. The structure of ER70S-6 in the composite is represented by the ferrite and the ferrite–cementite mixture (pearlite), and ER309LSI is represented by different ratios of austenite, δ-ferrite, carbide, and intermetallic phases. From the point of view of the mechanical properties, the most promising mode of surfacing is “Double Pulse”, followed by heat treatment by way of austenitization and normalization annealing. In this case, there is a decrease in the content of the δ-ferrite, a leveling of microhardness values, and a 40% increase in the tensile strength of the composite.
The Effects of Rumen-Protected Choline and Rumen-Protected Nicotinamide on Liver Transcriptomics in Periparturient Dairy Cows
Authors: Du Xue’er , Cui Zhijie , Zhang Rui , Zhao Keliang , Wang Lamei , Yao Junhu , Liu Shimin , Cai Chuanjiang , Cao Yangchun
Published: April 2023

To investigate the effects of rumen-protected choline (RPC) and rumen-protected nicotinamide (RPM) on liver metabolic function based on transcriptome in periparturient dairy cows, 10 healthy Holstein dairy cows with similar parity were allocated to RPC and RPM groups (n = 5). The cows were fed experimental diets between 14 days before and 21 days after parturition. The RPC diet contained 60 g RPC per day, and the RPM diet contained 18.7 g RPM per day. Liver biopsies were taken 21 days after calving for the transcriptome analysis. A model of fat deposition hepatocytes was constructed using the LO2 cell line with the addition of NEFA (1.6 mmol/L), and the expression level of genes closely related to liver metabolism was validated and divided into a CHO group (75 μmol/L) and a NAM group (2 mmol/L). The results showed that the expression of a total of 11,023 genes was detected and clustered obviously between the RPC and RPM groups. These genes were assigned to 852 Gene Ontology terms, the majority of which were associated with biological process and molecular function. A total of 1123 differentially expressed genes (DEGs), 640 up-regulated and 483 down-regulated, were identified between the RPC and RPM groups. These DEGs were mainly correlated with fat metabolism, oxidative stress and some inflammatory pathways. In addition, compared with the NAM group, the gene expression level of FGF21, CYP26A1, SLC13A5, SLCO1B3, FBP2, MARS1 and CDH11 in the CHO group increased significantly (p < 0.05). We proposed that that RPC could play a prominent role in the liver metabolism of periparturient dairy cows by regulating metabolic processes such as fatty acid synthesis and metabolism and glucose metabolism; yet, RPM was more involved in biological processes such as the TCA cycle, ATP generation and inflammatory signaling.
Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry (Rubus chamaemorus L.)
Authors: Faleva Anna V. , Ul’yanovskii Nikolay V. , Onuchina Aleksandra A. , Falev Danil I. , Kosyakov Dmitry S.
Published: April 2023

Cloudberry (Rubus chamaemorus L.) is a circumpolar boreal plant rich in bioactive compounds and is widely used in food and in folk medicine. In this study, a combination of two-dimensional NMR spectroscopy and liquid chromatography–high-resolution mass spectrometry was used for the comprehensive characterization of secondary metabolites in cloudberry lipophilic and hydrophilic extracts. Special attention was paid to the leaf extractives, which are highly enriched in polyphenolic compounds, the content of which reaches 19% in the extract (in gallic acid equivalent). The chemical composition of the polyphenolic fraction is represented mainly by the glycosylated derivatives of flavonoids, hydroxycinnamic (primarily caffeic), gallic (including the structure of galloyl ascorbate) and ellagic acids, catechin, and procyanidins. The contents of aglycones in the polyphenolic fraction were 64 and 100 mg g−1 for flavonoids and hydroxycinnamic acids, respectively, while the content of free caffeic acid was 1.2 mg g−1. This determines the exceptionally high antioxidant activity of this fraction (750 mg g−1 in gallic acid equivalent) and the ability to scavenge superoxide anion radicals, which is 60% higher than that of Trolox. The lower polar fractions consist mainly of glycolipids, which include polyunsaturated linolenic acid (18:3), pentacyclic triterpenic acids, carotenoid lutein, and chlorophyll derivatives, among which pheophytin a dominates. Along with the availability, the high antioxidant and biological activities of cloudberry leaf extracts make them a promising source of food additives, cosmetics, and pharmaceuticals.
Cytotoxicity and Identification of Antibacterial Compounds from Baillonella toxisperma Bark Using a LC-MS/MS and Molecular Networking Approach
Authors: Essono Mintsa Morel , Kumulungui Brice Serge , Obiang Cédric Sima , Dussert Elodie , Choque Elodie , Herfurth Damien , Ravallec Rozenn , Ondo Joseph-Privat , Mesnard François
Published: April 2023

Baillonella toxisperma is a medicinal plant used in northern Gabon to treat microbial diseases. It is a plant well-known by local populations, but very few studies have focused on the molecules responsible for the antibacterial activities of B. toxisperma. This study proposes a dereplication strategy based on molecular networking generated from HPLC-ESI-Q/TOF data, allowing investigation of the molecules responsible for the antibacterial activity of B. toxisperma. From this strategy, eighteen compounds were putatively identified. All of these compounds belonged mainly to five families of natural compounds, including phenylpropanolamines, stilbenes, flavonoids, lignans and phenolic glycosides. The chemical study carried out from the bark of B. toxisperma allowed us to identify, for the first time, compounds such as resveratrol and derivatives, epicatechin, epigallocatechin and epigallocatechin gallate. In addition, antibacterial activity (diffusion method and microdilution) and cytotoxicity (Cell Counting Kit-8 (CCK-8 Assay)) in vitro were evaluated. The crude ethanolic extract, as well as the fractions of B. toxisperma, showed significant antibacterial activity. However, the ethanolic fractions F2 and F4 presented high antibacterial activity compared to the crude extract. Cytotoxicity studies on colon-cancer cells (Caco-2) and human keratinocyte cells (HaCaT) showed moderate cytotoxicity in both cell types. This study clearly shows the therapeutic potential of the ethanolic extract of the bark of B. toxisperma and provides information on the phytochemical composition and bioactive compounds of the plant.
Analysis of Acousto-Optic Phenomenon in SAW Acoustofluidic Chip and Its Application in Light Refocusing
Authors: Qin Xianming , Chen Xuan , Yang Qiqi , Yang Lei , Liu Yan , Zhang Chuanyu , Wei Xueyong , Wang Weidong
Published: April 2023

This paper describes and analyzes a common acousto-optic phenomenon in surface acoustic wave (SAW) microfluidic chips and accomplishes some imaging experiments based on these analyses. This phenomenon in acoustofluidic chips includes the appearance of bright and dark stripes and image distortion. This article analyzes the three-dimensional acoustic pressure field and refractive index field distribution induced by focused acoustic fields and completes an analysis of the light path in an uneven refractive index medium. Based on the analysis of microfluidic devices, a SAW device based on a solid medium is further proposed. This MEMS SAW device can refocus the light beam and adjust the sharpness of the micrograph. The focal length can be controlled by changing the voltage. Moreover, the chip is also proven to be capable of forming a refractive index field in scattering media, such as tissue phantom and pig subcutaneous fat layer. This chip has the potential to be used as a planar microscale optical component that is easy to integrate and further optimize and provides a new concept about tunable imaging devices that can be attached directly to the skin or tissue.
Surface Acoustic Wave Humidity Sensor: A Review
Authors: Memon Maria Muzamil , Liu Qiong , Manthar Ali , Wang Tao , Zhang Wanli
Published: April 2023

The Growing demands for humidity detection in commercial and industrial applications led to the rapid development of humidity sensors based on different techniques. Surface acoustic wave (SAW) technology is one of these methods that has been found to provide a powerful platform for humidity sensing owing to its intrinsic features, including small size, high sensitivity, and simple operational mechanism. Similar to other techniques, the principle of humidity sensing in SAW devices is also realized by an overlaid sensitive film, which serves as the core element whose interaction with water molecules is responsible for overall performance. Therefore, most researchers are focused on exploring different sensing materials to achieve optimum performance characteristics. This article reviews sensing materials used to develop SAW humidity sensors and their responses based on theoretical aspects and experimental outcomes. Herein the influence of overlaid sensing film on the performance parameters of the SAW device, such as quality factor, signal amplitude, insertion loss, etc., is also highlighted. Lastly, a recommendation to minimize the significant change in device characteristics is presented, which we believe will be a good step for the future development of SAW humidity sensors.
A Two-Stage Screw Detection Framework for Automatic Disassembly Using a Reflection Feature Regression Model
Authors: Liu Quan , Deng Wupeng , Pham Duc Truong , Hu Jiwei , Wang Yongjing , Zhou Zude
Published: April 2023

For remanufacturing to be more economically attractive, there is a need to develop automatic disassembly and automated visual detection methods. Screw removal is a common step in end-of-life product disassembly for remanufacturing. This paper presents a two-stage detection framework for structurally damaged screws and a linear regression model of reflection features that allows the detection framework to be conducted under uneven illumination conditions. The first stage employs reflection features to extract screws together with the reflection feature regression model. The second stage uses texture features to filter out false areas that have reflection features similar to those of screws. A self-optimisation strategy and weighted fusion are employed to connect the two stages. The detection framework was implemented on a robotic platform designed for disassembling electric vehicle batteries. This method allows screw removal to be conducted automatically in complex disassembly tasks, and the utilisation of the reflection feature and data learning provides new ideas for further research.
Mixing in Two Types of Fluids Responsible for Some Carbonate-Hosted Pb–Zn Deposits, SW China: Insights from the Maoping Deposit
Authors: Wang Lei , Han Runsheng , Zhang Yan , Li Xiaodong
Published: April 2023

Carbonate-hosted Pb–Zn deposits are of major economic importance. The Sichuan–Yunnan–Guizhou metallogenetic belt (SYGMB), located on the western margin of the Yangtze Block, comprises over 400 carbonated-hosted Pb–Zn deposits. However, ore-forming fluids recorded in these deposits have led to controversy regarding ore genesis. We investigated a fluid system for the Maoping deposit in the SYGMB, based on fluid inclusions, and H–O–He–Ar isotopic studies. The results showed that ore-forming fluids in the Maoping deposit are characterized by mixing of high-temperature and low-salinity metamorphic fluids and low-temperature and high-salinity basinal brines. The Precambrian basement is considered to produce metamorphic fluids, while the basinal brines are said to originate from the Youjiang Basin. The mineralization at the depositional site appears to reflect the coincidence of the metamorphic fluids, basinal brines, Carboniferous coal seams, and structural-stratigraphic traps. Regional-scale data show large-scale heterogeneity in fluid properties, including basinal brines, metamorphic fluids, and organic fluids. Furthermore, the data suggest a precipitation model for some high-grade Pb–Zn deposits in the SYGMB. These findings will contribute to an understanding of deposit types in the SYGMB.
Simulating Bulk Ore Sorting Performance of a Panel Cave Mine: A Comparison between Two Approaches
Authors: Cetin Mahir Can , Li Genzhuang , Klein Bern , Futcher William
Published: April 2023

Conceptual bulk ore sorting studies are essential for determining a potential improvement in mine economics before undertaking on-site sensor trials. Two approaches, block modelling and drill core compositing, are applied to simulate the bulk ore sorting performance of mining operations. While one employs the grade data of a block model, the other approach utilizes composited drill core grades. This study aimed at comparing these two approaches by estimating in-situ grade heterogeneities and simulating the bulk ore sorting performances of the currently active caves of the Cadia East panel cave mine. The results show that block modelling tends to smooth the grade variability that initially exists in the drill core grade data. Particularly in the portions of the deposit where drilling is sparse or widely spaced compared to the selected block size, block modelling leads to lower grade heterogeneity and bulk ore sorting performance estimates. However, when the drill hole data is nonrepresentative of the area of interest, block modelling can predict more realistic bulk ore sorting performances compared to drill core grades. The assessments performed with the blocks and drill core composites of various sizes showed that grade heterogeneity was adversely affected by an increased sorting scale due to averaged metal grades.
Coherence of Bangui Magnetic Anomaly with Topographic and Gravity Contrasts across Central African Republic
Authors: Lemenkova Polina , Debeir Olivier
Published: April 2023

The interactions between the geophysical processes and geodynamics of the lithosphere play a crucial role in the geologic structure of the Earth’s crust. The Bangui magnetic anomaly is a notable feature in the lithospheric structure of the Central African Republic (CAR) resulting from a complex tectonic evolution. This study reports on the coherence in the geophysical data and magnetic anomaly field analysed from a series of maps. The data used here include raster grids on free-air altimetric gravity, magnetic EMAG2 maps, geoid EGM2008 model and topographic SRTM/ETOPO1 relief. The data were processed to analyse the correspondence between the geophysical and geologic setting in the CAR region. Histogram equalization of the topographic grids was implemented by partition of the raster grids into equal-area patches of data ranged by the segments with relative highs and lows of the relief. The original data were compared with the equalized, normalized and quadratic models. The scripts used for cartographic data processing are presented and commented. The consistency and equalization of topography, gravity and geoid data were based using GMT modules ‘grdfft’ and ‘grdhisteq’ modules. Using GMT scripts for mapping the geophysical and gravity data over CAR shows an advanced approach to multi-source data visualization to reveal the relationships in the geophysical and topographic processes in central Africa. The results highlighted the correlation between the distribution of rocks with high magnetism in the central part of the Bangui anomaly, and distribution of granites, greenstone belts, and metamorphosed basalts as rock exposure. The correspondence between the negative Bouguer anomaly (<−80 mGal), low geoid values (<−12 m) and the extent of the magnetic anomaly with extreme negative values ranging from −1000 to −200 nT is identified. The integration of the multi-source data provides new insights into the analysis of crustal thicknesses and the average density of the Earth in CAR, as well as the magnitude of the magnetic fields with notable deviations caused by the magnetic flux density in the Bangui area related to the distribution of mineral resources in CAR.
Anticancer Effect of STING Agonist-Encapsulated Liposomes on Breast Cancer
Authors: Zhang Jibing , Cui Xiao , Huang Yujiao , Xu Xiangdong , Feng Changshun , Li Jun
Published: April 2023

Breast cancer is one of the most common cancers worldwide, posing a serious threat to human health. Recently, innate immunity has become a widely discussed topic in antitumor research. The STING pathway is an important component of innate immunity, and several STING agonists have been developed and applied in antitumor research. Dimeric amidobenzimidazole (diABZI) is one STING agonist and is a nucleotide analog with low serological stability and cell membrane permeability. In this study, we prepared diABZI-encapsulated liposomes (dLNPs) using the ammonium sulfate gradient method. The average particle size of the dLNPs was 99.76 ± 0.230 nm, and the encapsulation efficiency was 58.29 ± 0.53%. Additionally, in vivo and in vitro assays showed that the dLNPs had a sustained-release effect and that the circulation time in vivo was longer than 48 h. The expression of IFN-β and IFN-γ was elevated in mice treated with dLNPs. Moreover, we found that dLNPs can recruit CD8+ T cells to tumor tissue and exert antitumor effects. The dLNPs-treated group showed the most significant efficacy: the average tumor volume was 231.46 mm3, which decreased by 78.16% and 54.47% compared to the PBS group and diABZI group. Meanwhile, the hemolysis rate of the dLNPs was 2%, showing high biocompatibility. In conclusion, dLNPs can effectively suppress tumor growth and possess great potential in breast cancer therapy.
Link between Flavor Perception and Volatile Compound Composition of Dark Chocolates Derived from Trinitario Cocoa Beans from Dominican Republic
Authors: Guzmán Penella Santiago , Boulanger Renaud , Maraval Isabelle , Kopp Gabi , Corno Marcello , Fontez Bénédicte , Fontana Angélique
Published: May 2023

The chemical composition of dark chocolate has a significant impact on its complex flavor profile. This study aims to investigate the relationship between the volatile chemical composition and perceived flavor of 54 dark chocolate samples made from Trinitario cocoa beans from the Dominican Republic. The samples were evaluated by a trained panel and analyzed using gas chromatography-mass spectrometry (GC-MS) to identify and quantify the volatile compounds. Predictive models based on a partial least squares regression (PLS) allowed the identification of key compounds for predicting individual sensory attributes. The models were most successful in classifying samples based on the intensity of bitterness and astringency, even though these attributes are mostly linked to non-volatile compounds. Acetaldehyde, dimethyl sulfide, and 2,3-butanediol were found to be key predictors for various sensory attributes, while propylene glycol diacetate was identified as a possible marker for red fruit aroma. The study highlights the potential of using volatile compounds to accurately predict chocolate flavor potential.
Comparison of Ten Metal-Doped LaFeO3 Samples on Photocatalytic Degradation of Antibiotics in Water under Visible Light: Role of Surface Area and Aqueous Phosphate Ions
Authors: Bolognino Isabella , Pelosato Renato , Marcì Giuseppe , Natali Sora Isabella
Published: May 2023

Doping semiconducting oxides, such as LaFeO3 (LF), with metallic elements is a good strategy to improve the performance of photocatalysts. In this study, LF and ten different nanopowders metal-doped at the La or Fe site of LaFeO3 were evaluated in the photocatalytic degradation of ciprofloxacin (CP) and oxytetracycline (OTC). The following metals were used in the doping (mol%) process of LF: Pd 3% and 5%; Cu 10%; Mg 5%, 10%, and 20%; Ga 10%; Y 10% and 20%; and Sr 20%. The doped samples were synthetized using a citrate auto-combustion technique. From the X-ray diffraction (XRD) data, only a single crystalline phase, namely an orthorhombic perovskite structure, was observed except for trace amounts of PdO in the sample with Pd 5%. The specific surface area (SSA) ranged from 9 m2 g−1 (Ga 10%) to 20 m2 g−1 (Mg 20%). SEM images show that all samples were constituted from agglomerates of particles whose sizes ranged from ca. 20 nm (Mg 20%) to ca. 100 nm (Pd 5%). Dilute aqueous solutions (5 × 10−6 M) prepared for both CP and OTC were irradiated for 240 min under visible-light and in the presence of H2O2 (10−2 M). The results indicate a 78% removal of OTC with Cu 10% doped LF in a phosphate buffer (pH = 5.0). The degradation of CP is affected by pH and phosphate ions, with 78% (in unbuffered solution) and 54% (in phosphate buffer, pH = 5.0) removal achieved with Mg 10% doped LF. The reactions follow a pseudo-first order kinetic. Overall, this study is expected to deepen the assessment of photocatalytic activity by using substrates with different absorption capacities on photocatalysts.
Reactive Disperse Dyes Bearing Various Blocked Isocyanate Groups for Digital Textile Printing Ink
Authors: Jeong Subin , Kim Giyoung , Bae Hyoungeun , Kim Hyeokjin , Seo Eunjeong , Choi Sujeong , Jeong Jieun , Jung Hyocheol , Lee Sangho , Cheong Inwoo , Kim Jinchul , Park Youngil
Published: May 2023

Wastewater management is of considerable economic and environmental importance for the dyeing industry. Digital textile printing (DTP), which is based on sublimation transfer and does not generate wastewater, is currently being explored as an inkjet-based method of printing colorants onto fabric. It finds wide industrial applications with most poly(ethylene terephthalate) (PET) and nylon fibers. However, for additional industrial applications, it is necessary to use natural fibers, such as cotton. Therefore, to expand the applicability of DTP, it is essential to develop a novel reactive disperse dye that can interact with the fabric. In this study, we introduced a blocked isocyanate functional group into the dye to enhance binding to the fabric. The effect of sublimation transfer on fabrics as a function of temperature was compared using the newly synthesized reactive disperse dyes with different blocking groups based on pyrazole derivatives, such as pyrazole (Py), di-methylpyrazole (DMPy), and di-tert-butylpyrazole (DtBPy). Fabrics coated with the new reactive disperse dyes, including PET, nylon, and cotton, were printed at 190 °C, 200 °C, and 210 °C using thermal transfer equipment. In the case of the synthesized DHP-A dye on cotton at 210 °C, the color strength was 2.1, which was higher than that of commercial dyes and other synthesized dyes, such as DMP-A and DTP-A. The fastness values of the synthesized DHP-A were measured on cotton, and it was found that the washing and light fastness values on cotton are higher than those of commercial dyes. This study confirmed the possibility of introducing isocyanate groups into reactive disperse dyes.
Abscopal Effect, Extracellular Vesicles and Their Immunotherapeutic Potential in Cancer Treatment
Authors: Salazar Aleli , Chavarria Víctor , Flores Itamar , Ruiz Samanta , Pérez de la Cruz Verónica , Sánchez-García Francisco Javier , Pineda Benjamin
Published: May 2023

The communication between tumor cells and the microenvironment plays a fundamental role in the development, growth and further immune escape of the tumor. This communication is partially regulated by extracellular vesicles which can direct the behavior of surrounding cells. In recent years, it has been proposed that this feature could be applied as a potential treatment against cancer, since several studies have shown that tumors treated with radiotherapy can elicit a strong enough immune response to eliminate distant metastasis; this phenomenon is called the abscopal effect. The mechanism behind this effect may include the release of extracellular vesicles loaded with damage-associated molecular patterns and tumor-derived antigens which activates an antigen-specific immune response. This review will focus on the recent discoveries in cancer cell communications via extracellular vesicles and their implication in tumor development, as well as their potential use as an immunotherapeutic treatment against cancer.
Effect of Eleutheroside E on an MPTP-Induced Parkinson’s Disease Cell Model and Its Mechanism
Authors: Yao Yi , Liao Caiyu , Qiu Honghao , Liang Lishan , Zheng Wenying , Wu Liyan , Meng Fanxin
Published: May 2023

This research investigated the effects of eleutheroside E (EE) on the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease cell model and its mechanism. Methods: To create a cell model of Parkinson’s disease, MPTP (2500 μmol/L) was administered to rat adrenal pheochromocytoma cells (PC-12) to produce an MPTP group. Selegiline (50 μmol/L) and MPTP had been administered to the positive group beforehand. The eleutheroside E group was divided into low-, medium-, and high-concentration groups, in which the cells were pretreated with eleutheroside E at concentrations of 100 μmol/L, 300 μmol/L, and 500 μmol/L. Next, MPTP was added to the cells separately. The CCK-8 method was used to measure the cell survival rate. Apart from the CCK-8 method, mitochondrial membrane potential detection, cell reactive oxygen species (ROS) detection, and other methods were also adopted to verify the effect of low, medium, and high concentrations of eleutheroside E on the MPTP-induced cell model. Western blot analysis was used to detect changes in the expression of intracellular proteins CytC, Nrf2, and NQO1 to clarify the mechanism. The results are as follows. Compared with the MPTP group, the survival rates of cells at low, medium, and high concentrations of eleutheroside E all increased. The mitochondrial membrane potential at medium and high concentrations of eleutheroside E increased. The ROS levels at medium and high concentrations of eleutheroside E decreased. Moreover, the apoptosis rate decreased and the expression levels of the intracellular proteins CytC, Nrf2, and NQO1 were upregulated. Conclusion: Eleutheroside E can improve the MPTP-induced apoptosis of PC-12 cells by increasing the mitochondrial membrane potential and reducing the level of intracellular reactive oxygen species (ROS). Moreover, the apoptosis of cells is regulated by the expression of CytC, Nrf2, and NQO1 proteins.
α-Glucosidase Inhibitors from Two Mangrove-Derived Actinomycetes
Authors: Lu Xuejun , Zhang Manlai , Qiu Yixian , Liu Xiuxiu , Wang Cancan , Chen Jianwei , Zhang Huawei , Wei Bin , Yu Yanlei , Ying Youmin , Hong Kui , Wang Hong
Published: May 2023

α-Glucosidase (AGS) inhibitors have been regarded as an ideal target for the management of type 2 diabetes mellitus (T2DM) since they can maintain an acceptable blood glucose level by delaying the digestion of carbohydrates and diminishing the absorption of monosaccharides. In the process of our endeavor in mining AGS inhibitors from natural sources, the culture broth of two mangrove-derived actinomycetes Streptomyces sp. WHUA03267 and Streptomyces sp. WHUA03072 exhibited an apparent inhibitory activity against AGS. A subsequent chemical investigation into the two extracts furnished 28 secondary metabolites that were identified by spectroscopic methods as two previously undescribed linear polyketides 1–2, four benzenoid ansamycins 3–6, fourteen cyclodipeptides 7–18, one prenylated indole derivative 19, two fusicoccane-type diterpenoids 20–21, two hydroxamate siderophore 22–23, and five others 24–28. Among all of the isolates, 11 and 24 were obtained from actinomycetes for the first time, while 20–21 had never been reported to occur in a marine-derived microorganism previously. In the in vitro AGS inhibitory assay, compounds 3, 8, 9, 11, 14, 16, and 17 exhibited potent to moderate activity with IC50 values ranging from 35.76 ± 0.40 to 164.5 ± 15.5 μM, as compared with acarbose (IC50 = 422.3 ± 8.4 μM). The AGS inhibitory activity of 3, 9, 14, 16, and 17 was reported for the first time. In particular, autolytimycin (3) represented the first ansamycin derivative reported to possess the AGS inhibitory activity. Kinetics analysis and molecular docking were performed to determine the inhibition types and binding modes of these inhibitors, respectively. In the MTT assay, 3, 8, 9, 11, 14, 16, and 17 exhibited no apparent cytotoxicity to the human normal hepatocyte (LO2) cells, suggesting satisfactory safety of these AGS inhibitors.
Lipid Mediators Metabolic Chaos of Asthmatic Mice Reversed by Rosmarinic Acid
Authors: Qin Tuo , Rong Xiaojuan , Zhang Xiaohui , Kong Lingfei , Kang Yutong , Liu Xuanlin , Hu Mengying , Liang Handong , Tie Cai
Published: May 2023

Background and objective: Asthma is a common chronic inflammatory disease of the airways with no known cure. Lipid mediators (LMs) are a kind of inflammatory signaling molecules which are believed to be involved in the development of asthma. Hyssopus cuspidatus Boriss. is a traditional Uyghur medicine, which is widely used in the treatment of asthma and other respiratory diseases. Extraction of Hyssopus cuspidatus Boriss. was reported to neutralize asthma symptoms. The purpose of the study was to investigate both the anti-inflammatory and immunoregulation properties of the Hyssopus cuspidatus Boriss. extract (SXCF) and its main active constituent, rosmarinic acid (RosA), in vivo. The effect of RosA, a major constituent of SXCF, was evaluated on an asthmatic model, with both anti-inflammatory and immunoregulation properties. Materials and methods: Anti-inflammatory effect of SXCF and RosA was assessed using OVA-induced asthma model mice by UPLC-MS/MS method. Results: Overall, RosA played a critical role in anti-asthma treatment. In total, 90% of LMs species that were significantly regulated by SXCF were covered. On the most important LMs associated with asthma, RosA equivalent induced similar effects as SXCF did. It is believed that some constituents in SXCF could neutralize RosA excessive impacts on LMs.
Redesigning Carbon–Carbon Backbone Polymers for Biodegradability–Compostability at the End-of-Life Stage
Authors: Mulchandani Neha , Narayan Ramani
Published: May 2023

Carbon–carbon backbone polymers are non-biodegradable, persistent plastics that have accumulated on land and oceans due to human activities. They degrade and fragment into microplastics and smaller particle sizes but do not biodegrade at an acceptable and practical rate. Their continual buildup in the natural environment precipitates serious detrimental impacts on human health and the environment, as extensively documented in the literature and media. Nearly 77% of global plastics produced are carbon–carbon backbone polymers. More importantly, 90% of packaging plastics (153.8 million metric tons) are non-biodegradable, persistent carbon–carbon backbone polymers. The recycling rate of these non-durable packaging plastics ranges from 0 to 4%. Re-designing carbon–carbon backbone polymers to labile ester backbone biodegradable–compostable polymers and treating them along with biodegradable organic waste (such as food, paper, and organic wastes) in managed industrial composting is environmentally responsible. Diverting 1 million metric tons of biodegradable organic wastes in MSW bound for landfills and open dumps to industrial composting results in 0.95 million metric tons CO2 equivalents of GHG emissions reduction. This perspective paper discusses strategies and rationales regarding the redesign of carbon–carbon backbone polymer molecules. It describes the carbon footprint reductions achievable by replacing petro-fossil carbon with plant biomass carbon. Biodegradability and compostability are frequently used but misunderstood and misused terms, leading to misleading claims in the marketplace. This paper presents the fundamentals of biodegradability and compostability of plastics and the requirements to be met according to ASTM/ISO international standards.
Synthesis and Structural Characterization of p-Carboranylamidine Derivatives
Authors: Harmgarth Nicole , Liebing Phil , Lorenz Volker , Engelhardt Felix , Hilfert Liane , Busse Sabine , Goldhahn Rüdiger , Edelmann Frank T.
Published: May 2023

In this contribution, the first amidinate and amidine derivatives of p-carborane are described. Double lithiation of p-carborane (1) with n-butyllithium followed by treatment with 1,3-diorganocarbodiimides, R–N=C=N–R (R = iPr, Cy (= cyclohexyl)), in DME or THF afforded the new p-carboranylamidinate salts p-C2H10B10[C(NiPr)2Li(DME)]2 (2) and p-C2H10B10[C(NCy)2Li(THF)2]2 (3). Subsequent treatment of 2 and 3 with 2 equiv. of chlorotrimethylsilane (Me3SiCl) provided the silylated neutral bis(amidine) derivatives p-C2H10B10[C{iPrN(SiMe3)}(=NiPr)]2 (4) and p-C2H10B10[C{CyN(SiMe3)}(=NCy)]2 (5). The new compounds 3 and 4 have been structurally characterized by single-crystal X-ray diffraction. The lithium carboranylamidinate 3 comprises a rare trigonal planar coordination geometry around the lithium ions.
The Potential Use of Plant Growth Regulators for Modification of the Industrially Valuable Volatile Compounds Synthesis in Hylocreus undatus Stems
Authors: Jakobina Maciej , Łyczko Jacek , Zydorowicz Kinga , Galek Renata , Szumny Antoni
Published: May 2023

The pitaya (dragon fruit) Hylocereus is a genus which belongs to the Cactaceae family. It is native to Mexico, occurring also in other regions of Central and South America. Pitaya fruit is mainly intended for consumption and for this reason the species is grown commercially. The fruit is a rich source of vitamins, biologically active compounds, and dietary fibre. Using in vitro culture can accelerate the process of reproduction and growth of pitaya plants. Profiling of volatile compounds contained in the stem of Hylocereus undatus was carried out using the SPME-GC-MS technique. The main compounds present were hexanal, 2-hexenal and 1-hexanol. The results showed differences in the occurrence of volatile compounds between plants grown in media with an addition of BA (6-benzylaminopurine) and IAA (indole-3-acetic acid), which have been used as plant growth regulators. Statistically significant differences between the contents of volatile compounds were observed in the case of 2-hexenal and 1-hexanol. The effect of BA on reducing the amount of volatile compounds was observed. However, introduction of IAA to the in vitro medium resulted in more compounds being synthesized. This study is the first to describe the volatile compounds in the pitaya stem. The results indicate that plant hormones are able to modify the profile of volatile compounds.
Studies of Dopamine Oxidation Process by Atmospheric Pressure Glow Discharge Mass Spectrometry
Authors: Dai Dongli , Zhu Yueqin , Zhu Zhenli , Qian Rong , Zhuo Shangjun , Liu Anqi , Li Xian , Li Wei , Chen Qiao
Published: May 2023

An atmospheric pressure glow discharge ionisation source was constructed and utilized to study the dopamine (DA) oxidation process coupling with mass spectrometry. During the DA oxidation process catalysed by polyphenol oxidase (PPO), six cationic intermediates were directly detected by the atmospheric pressure glow discharge mass spectrometry (APGD-MS). Combined with tandem mass spectrometry, the structures of the dopamine o-semiquinone radical (DASQ) and leukodopaminochrome radical (LDAC●) intermediates and structures of the isomers of dopaminochrome (DAC) and 5,6-dihydroxyindole (DHI) were further characterised with the introduction of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) and deuterium oxide (D2O) to APGD-MS. Meanwhile, UV–Vis studies confirmed the important role of PPO in catalyzing the DA oxidation reaction. Based on APGD-MS studies, a possible mechanism could be proposed for DA oxidation catalysed by PPO. Furthermore, APGD-MS could provide possibilities for the effective detection and characterisation of short-lived intermediates, even in complicated systems.
Chemical Characterisation, Antidiabetic, Antibacterial, and In Silico Studies for Different Extracts of Haloxylon stocksii (Boiss.) Benth: A Promising Halophyte
Authors: Rizvi Syed Nabil Raza , Afzal Samina , Khan Kashif-ur-Rehman , Aati Hanan Y. , Rao Huma , Ghalloo Bilal Ahmad , Shahzad Muhammad Nadeem , Khan Duraiz Ahmed , Esatbeyoglu Tuba , Korma Sameh A.
Published: May 2023

The objective of the study is to evaluate the chemical characterisation, and biological and in silico potential of Haloxylon stocksii (Boiss.) Benth, an important halophyte commonly used in traditional medicine. The research focuses on the roots and aerial parts of the plant and extracts them using two solvents: methanol and dichloromethane. Chemical characterisation of the extracts was carried out using total phenolic contents quantification, GC-MS analysis, and LC-MS screening. The results exhibited that the aerial parts of the plant have significantly higher total phenolic content than the roots. The GC-MS and LC-MS analysis of the plant extracts revealed the identification of 18 bioactive compounds in each. The biological evaluation was performed using antioxidant, antibacterial, and in vitro antidiabetic assays. The results exhibited that the aerial parts of the plant have higher antioxidant and in vitro antidiabetic activity than the roots. Additionally, the aerial parts of the plant were most effective against Gram-positive bacteria. Molecular docking was done to evaluate the binding affinity (BA) of the bioactive compounds characterised by GC-MS with diabetic enzymes used in the in vitro assay. The results showed that the BA of γ-sitosterol was better than that of acarbose, which is used as a standard in the in vitro assay. Overall, this study suggests that the extract from aerial parts of H. stocksii using methanol as a solvent have better potential as a new medicinal plant and can provide a new aspect to develop more potent medications. The research findings contribute to the scientific data of the medicinal properties of Haloxylon stocksii and provide a basis for further evaluation of its potential as a natural remedy.
Real-Time Flood Forecasting and Warning: A Comprehensive Approach toward HCI-Centric Mobile App Development
Authors: Alsabhan Waleed , Dudin Basil
Published: April 2023

This article discusses the design, development, and usability assessment of a mobile system for producing hydrological predictions and sending flood warnings in response to the desire for human-centered technology to better the management of flood occurrences. Our work acts as a bibliographic reference for understanding what others have attempted and found, as well as gives an integrated set of recommendations. Furthermore, our guidelines offer guidance to aid in the design of mobile GIS-based hydrological models for mobile devices. We concentrate on the full design of a human–computer interaction framework for an effective flood prediction and warning system. In addition, we analyze and address the current user needs and requirements for building a user interface for mobile real-time flood forecasting in a methodical manner. Although a functional prototype was created, the primary objective of this research was to comprehend the complexity of possible users’ demands and actual use situations in order to solve the problem of comparable systems being difficult to use. After consulting with possible consumers, application design standards were established and implemented in the initial prototype. Focusing on user demands and attitudes, special consideration was given to the usability of the mobile interface. To develop the application, a variety of assessment methods are added. The conclusion of the examination was that the system is efficient and effective.
The Effect of Lithium Ion Leaching from Calcined Li–Al Hydrotalcite on the Rapid Removal of Ni2+/Cu2+ from Contaminated Aqueous Solutions
Authors: Chen Yu-Jia , Uan Jun-Yen
Published: April 2023

A layered double hydroxide (LDH) calcined-framework adsorbent was investigated for the rapid removal of heavy metal cations from plating wastewater. Li–Al–CO3 LDH was synthesized on an aluminum lathe waste frame surface to prepare the sorbent. The calcination treatment modified the LDH surface properties, such as the hydrophilicity and the surface pH. The change in surface functional groups and the leaching of lithium ions affected the surface properties and the adsorption capacity of the heavy metal cations. A zeta potential analysis confirmed that the 400 °C calcination changed the LDH surface from positively charged (+10 mV) to negatively charged (−17 mV). This negatively charged surface contributed to the sorbent instantly bonding with heavy metal cations in large quantities, as occurs during contact with wastewater. The adsorption isotherms could be fitted using the Freundlich model. The pseudo-second-order model and the rate-controlled liquid-film diffusion model successfully simulated the adsorption kinetics, suggesting that the critical adsorption step was a heterogeneous surface reaction. This study also confirmed that the recovered nickel and/or copper species could be converted into supported metal nanoparticles with a high-temperature hydrogen reduction treatment, which could be reused as catalysts.
Intergranular Spin Dependent Tunneling Dominated Magnetoresistance in Helimagnetic Manganese Phosphide Thin Films
Authors: Muchharla Baleeswaraiah , Madhogaria Richa Pokharel , Detellem Derick , Hung Chang-Ming , Chanda Amit , Mudiyanselage Nivarthana W. Y. A. Y. , Duong Anh Tuan , Trinh Minh-Tuan , Witanachchi Sarath , Phan Manh-Huong
Published: April 2023

Helical magnets are emerging as a novel class of materials for spintronics and sensor applications; however, research on their charge- and spin-transport properties in a thin film form is less explored. Herein, we report the temperature and magnetic field-dependent charge transport properties of a highly crystalline MnP nanorod thin film over a wide temperature range (2 K < T < 350 K). The MnP nanorod films of ~100 nm thickness were grown on Si substrates at 500 °C using molecular beam epitaxy. The temperature-dependent resistivity ρ(T) data exhibit a metallic behavior (dρ/dT > 0) over the entire measured temperature range. However, large negative magnetoresistance (Δρ/ρ) of up to 12% is observed below ~50 K at which the system enters a stable helical (screw) magnetic state. In this temperature regime, the Δρ(H)/ρ(0) dependence also shows a magnetic field-manipulated CONE + FAN phase coexistence. The observed magnetoresistance is dominantly governed by the intergranular spin dependent tunneling mechanism. These findings pinpoint a correlation between the transport and magnetism in this helimagnetic system.
Chitin-Derived Nitrogen-Doped Carbon Nanopaper with Subwavelength Nanoporous Structures for Solar Thermal Heating
Authors: Yeamsuksawat Thanakorn , Zhu Luting , Kasuga Takaaki , Nogi Masaya , Koga Hirotaka
Published: April 2023

Sustainable biomass-derived carbons have attracted research interest because of their ability to effectively absorb and convert solar light to thermal energy, a phenomenon known as solar thermal heating. Although their carbon-based molecular and nanoporous structures should be customized to achieve enhanced solar thermal heating performance, such customization has insufficiently progressed. In this study, we transformed a chitin nanofiber/water dispersion into paper, referred to as chitin nanopaper, with subwavelength nanoporous structures by spatially controlled drying, followed by temperature-controlled carbonization without any pretreatment to customize the carbon-based molecular structures. The optimal carbonization temperature for enhancing the solar absorption and solar thermal heating performance of the chitin nanopaper was determined to be 400 °C. Furthermore, we observed that the nitrogen component, which afforded nitrogen-doped carbon structures, and the high morphological stability of chitin nanofibers against carbonization, which maintained subwavelength nanoporous structures even after carbonization, contributed to the improved solar absorption of the carbonized chitin nanopaper. The carbonized chitin nanopaper exhibited a higher solar thermal heating performance than the carbonized cellulose nanopaper and commercial nanocarbon materials, thus demonstrating significant potential as an excellent solar thermal material.