bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection and Analysis of VOCs in Cherry Tomato Based on GC-MS and GC×GC-TOF MS Techniques
Authors: Guan Sihui , Liu Chenxu , Yao Zhuping , Wan Hongjian , Ruan Meiying , Wang Rongqing , Ye Qingjing , Li Zhimiao , Zhou Guozhi , Cheng Yuan
Published: April 2024

Volatile organic compounds (VOCs) play a significant role in influencing the flavor quality of cherry tomatoes (Solanum lycopersicum var. cerasiforme). The scarcity of systematic analysis of VOCs in cherry tomatoes can be attributed to the constraints imposed by detection technology and other contributing factors. In this study, the cherry tomato cultivar var. ‘Zheyingfen1’ was chosen due to its abundant fruit flavor. Two detection technology platforms, namely the commonly employed headspace solid-phase microextraction—gas chromatography–mass spectrometry (HS-SPME-GC-MS) and the most advanced headspace solid-phase microextraction—full two-dimensional gas chromatography–time-of-flight mass spectrometry (HS-SPME-GC×GC-TOFMS), were employed in the analysis. The VOCs of cherry tomato cultivar var. ‘Zheyingfen1’ fruits at red ripening stage were detected. A combined total of 1544 VOCs were detected using the two aforementioned techniques. Specifically, 663 VOCs were identified by through the HS-SPME-GC-MS method, 1026 VOCs were identified by through the HS-SPME-GC×GC-TOFMS, and 145 VOCs were identified by both techniques. The identification of β-ionone and (E)-2-nonenal as the principal VOCs was substantiated through the application of the relative odor activity value (rOAV) calculation and subsequent analysis. Based on the varying contribution rates of rOAV, the analysis of sensory flavor characteristics revealed that cherry tomato cultivar var. ‘Zheyingfen1’ predominantly exhibited green and fatty attributes, accompanied by elements of fresh and floral flavor characteristics. In conclusion, our study conducted a comprehensive comparison of the disparities between these two methodologies in detecting VOCs in cherry tomato fruits. Additionally, we systematically analyzed the VOC composition and sensory flavor attributes of the cherry tomato cultivar var. ‘Zheyingfen1’. This research serves as a significant point of reference for investigating the regulatory mechanisms underlying the development of volatile flavor quality in cherry tomatoes.
A Facile Determination of Herbicide Residues and Its Application in On-Site Analysis
Authors: Sun Yifei , Tang Yan , Chen Zetao , Ge Miaoxiu , Xiong Wei , Wen Luhong
Published: April 2024

Abuse of herbicides in food safety is a vital concern that has an influence on the sustainable development of the world. This work presents, a modified ionization method with separation of the sample and carrier gas inlets, which was utilized for efficient ionization and analyte transfer of herbicides in crops. The working parameters of voltage, injective distance, desorption temperature, and the carrier gas flow rate were optimized to achieve the high efficiency of the transfer and ionization of the analyte. When it was applied in the analysis of herbicides in laboratory, the method exhibited excellent performance in achieving the quantitative detection of herbicides in solutions and residues spiked in an actual matrix with a limit of quantification of 1–20 μg/kg and relative standard deviations of less than 15%. Although a simple QuEchERS process was used, the programmable heating platform ensured efficient gasification and transfer of the target analyte, with the advantages of high speed and selectivity, avoiding the noted matrix effect. The method exhibited a relatively acceptable performance by using air as the discharged gas (open air). It could be used to monitor herbicide residues in the growth stage via on-site non-destructive analysis, which obtained low LODs by dissociating the herbicides from the crops without any pretreatment. It showed great potential for the supervision of the food safety market by achieving non-destructive detection of crops anytime and anywhere. This finding may provide new insights into the determination of pesticide emergence and rice quality assessment.
A Longitudinal Analysis of the Impact of Digital Technologies on Sustainable Food Production and Consumption in the European Union
Authors: Bocean Claudiu George
Published: April 2024

In today’s landscape, digital technologies hold immense potential in tackling challenges associated with food sustainability. This study aims to contextualize a broader investigation of food sustainability and digitalization within the agricultural sector. Its objective is to explore the influence of digital technologies on sustainable food production and consumption, particularly examining relationships among digital technologies, municipal waste, agricultural output, nitrogen emissions, methane emissions from agriculture, and Goal 12 Responsible Consumption and Production (SDG12). Through the use of Structural Equation Modeling, the empirical investigation scrutinizes the relationships between digital technology use and critical variables linked to food sustainability in a longitudinal analysis. The results highlight the significant impact of extensive digital technology use on municipal waste, sustainable production, and consumption, indirectly influencing greenhouse gas (GHG) emissions. Empirical research findings reveal a negative influence of digital technologies on responsible consumption and production (path coefficient −0.349, p values < 0.001), suggesting an impact of digital technologies on diminishing sustainability in consumption and production. The relationship between digital technologies and municipal solid waste is also negative (path coefficient −0.360, p values < 0.001), suggesting that the use of digital technologies can contribute to reducing the amount of municipal solid waste. Digitalization has the potential to improve the sustainability of supply chains by reducing resource consumption and greenhouse gas emissions associated with production and distribution operations.
Extraction, Identification, and In Vitro Anti-Inflammatory Activity of Feruloylated Oligosaccharides from Baijiu Distillers’ Grains
Authors: Yin Zhongtian , Liu Mengyao , Wang Bowen , Zhao Dongrui , Li Hehe , Sun Jinyuan
Published: April 2024

The structure and function of phenoyl oligosaccharides in baijiu distillers’ grains (BDGs) have not been identified and investigated yet. This study aimed to elucidate the major phenolic oligosaccharides present in BDGs, optimize their extraction process via a central composite design, and assess their anti-inflammatory properties utilizing the LPS-induced RAW264.7 inflammation model. The main results are as follows: feruloylated oligosaccharides (FOs) were identified as the main phenoyl oligosaccharides in BDGs with a structure of ferulic acid esterified on arabinooligosaccharide xylose. Then, the preparation process of FOs was optimized using the following conditions: pH 5, temperature 55 °C, time 12 h, xylanase addition amount 7 g/L, BDG concentration 120 g/L. Furthermore, the acquired FOs demonstrated notable scavenging activity against DPPH and ABTS free radicals, with Trolox equivalent values of 366.8 ± 10.38 and 0.35 ± 0.01 mM Trolox/mg sample, respectively. However, their efficacy was comparatively lower than that of ferulic acid. Finally, the obtained FOs could effectively inhibit the LPS-induced secretion of TNF-α, IL-6, and IL-1β and promote the secretion of IL-10 in RAW264.7 cells. Based on the above results, FOs from BDGs were determined to have certain antioxidant and anti-inflammatory activities.
Unravelling the Fractal Complexity of Temperature Datasets across Indian Mainland
Authors: Sankaran Adarsh , Plocoste Thomas , Geetha Raveendran Nair Arathy Nair , Mohan Meera Geetha
Published: April 2024

Studying atmospheric temperature characteristics is crucial under climate change, as it helps us to understand the changing patterns in temperature that have significant implications for the environment, ecosystems, and human well-being. This study presents the comprehensive analysis of the spatiotemporal variability of scaling behavior of daily temperature series across the whole Indian mainland, using a Multifractal Detrended Fluctuation Analysis (MFDFA). The analysis considered 1° × 1° datasets of maximum temperature (Tmax), minimum temperature (Tmin), mean temperature (Tmean), and diurnal temperature range (DTR) (TDTR = Tmax − Tmin) from 1951 to 2016 to compare their scaling behavior for the first time. Our results indicate that the Tmin series exhibits the highest persistence (with the Hurst exponent ranging from 0.849 to unity, and a mean of 0.971), and all four-temperature series display long-term persistence and multifractal characteristics. The variability of the multifractal characteristics is less significant in North–Central India, while it is highest along the western coast of India. Moreover, the assessment of multifractal characteristics of different temperature series during the pre- and post-1976–1977 period of the Pacific climate shift reveals a notable decrease in multifractal strength and persistence in the post-1976–1977 series across all regions. Moreover, for the detection of climate change and its dominant driver, we propose a new rolling window multifractal (RWM) framework by evaluating the temporal evolution of the spectral exponents and the Hurst exponent. This study successfully captured the regime shifts during the periods of 1976–1977 and 1997–1998. Interestingly, the earlier climatic shift primarily mitigated the persistence of the Tmax series, whereas the latter shift significantly influenced the persistence of the Tmean series in the majority of temperature-homogeneous regions in India.
In Vitro Antibacterial and Anti-Inflammatory Properties of Imidazolium Poly(ionic liquids) Microspheres Loaded in GelMA-PEG Hydrogels
Authors: Zhou Chao , Sun Mengdi , Wang Danni , Yang Mingmei , Loh Jia Ling Celestine , Xu Yawen , Zhang Ruzhi
Published: April 2024

Repairing damaged tissue caused by bacterial infection poses a significant challenge. Traditional antibacterial hydrogels typically incorporate various components such as metal antimicrobials, inorganic antimicrobials, organic antimicrobials, and more. However, drawbacks such as the emergence of multi-drug resistance to antibiotics, the low antibacterial efficacy of natural agents, and the potential cytotoxicity associated with metal antibacterial nanoparticles in hydrogels hindered their broader clinical application. In this study, we successfully developed imidazolium poly(ionic liquids) (PILs) polymer microspheres (APMs) through emulsion polymerization. These APMs exhibited notable antibacterial effectiveness and demonstrated minimal cell toxicity. Subsequently, we integrated the APMs into a gelatin methacryloyl (GelMA)—polyethylene glycol (PEG) hydrogel. This composite hydrogel not only showcased strong antibacterial and anti-inflammatory properties but also facilitated the migration of human skin fibroblasts (HSF) and human umbilical vein endothelial cells (HUVECs) and promoted osteogenic differentiation in vitro.
Liposome–Hydrogel Composites for Controlled Drug Delivery Applications
Authors: Binaymotlagh Roya , Hajareh Haghighi Farid , Chronopoulou Laura , Palocci Cleofe
Published: April 2024

Various controlled delivery systems (CDSs) have been developed to overcome the shortcomings of traditional drug formulations (tablets, capsules, syrups, ointments, etc.). Among innovative CDSs, hydrogels and liposomes have shown great promise for clinical applications thanks to their cost-effectiveness, well-known chemistry and synthetic feasibility, biodegradability, biocompatibility and responsiveness to external stimuli. To date, several liposomal- and hydrogel-based products have been approved to treat cancer, as well as fungal and viral infections, hence the integration of liposomes into hydrogels has attracted increasing attention because of the benefit from both of them into a single platform, resulting in a multifunctional drug formulation, which is essential to develop efficient CDSs. This short review aims to present an updated report on the advancements of liposome–hydrogel systems for drug delivery purposes.
Contradiction in Star-Allele Nomenclature of Pharmacogenes between Common Haplotypes and Rare Variants
Authors: Ahn Se Hwan , Park Yoomi , Kim Ju Han
Published: April 2024

The nomenclature of star alleles has been widely used in pharmacogenomics to enhance treatment outcomes, predict drug response variability, and reduce adverse reactions. However, the discovery of numerous rare functional variants through genome sequencing introduces complexities into the star-allele system. This study aimed to assess the nature and impact of the rapid discovery of numerous rare functional variants in the traditional haplotype-based star-allele system. We developed a new method to construct haplogroups, representing a common ancestry structure, by iteratively excluding rare and functional variants of the 25 representative pharmacogenes using the 2504 genomes from the 1000 Genomes Project. In total, 192 haplogroups and 288 star alleles were identified, with an average of 7.68 ± 4.2 cross-ethnic haplogroups per gene. Most of the haplogroups (70.8%, 136/192) were highly aligned with their corresponding classical star alleles (VI = 1.86 ± 0.78), exhibiting higher genetic diversity than the star alleles. Approximately 41.3% (N = 119) of the star alleles in the 2504 genomes did not belong to any of the haplogroups, and most of them (91.3%, 105/116) were determined by a single variant according to the allele-definition table provided by CPIC. These functional single variants had low allele frequency (MAF < 1%), high evolutionary conservation, and variant deleteriousness, which suggests significant negative selection. It is suggested that the traditional haplotype-based naming system for pharmacogenetic star alleles now needs to be adjusted by balancing both traditional haplotyping and newly emerging variant-sequencing approaches to reduce naming complexity.
Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm
Authors: Ding Xiaoyu , Tian Yongsheng , Qiu Yishu , Duan Pengfei , Wang Xinyi , Li Zhentong , Li Linlin , Liu Yang , Wang Linna
Published: April 2024

The giant grouper fish (Epinephelus lanceolatus), one of the largest and rarest groupers, is a fast-growing economic fish. Grouper sperm is often used for cross-breeding with other fish and therefore sperm cryopreservation is important. However, freezing damage cannot be avoided. Herein, we performed a transcriptome analysis to compare fresh and frozen sperm of the giant grouper with frozen storage times of 0, 23, 49, and 61 months. In total, 1911 differentially expressed genes (DEGs), including 91 in El-0-vs-El-23 (40 upregulated and 51 downregulated), 251 in El-0-vs-El-49 (152 upregulated and 69 downregulated), and 1569 in El-0-vs-El-61 (984 upregulated and 585 downregulated), were obtained in the giant grouper sperm. DEGs were significantly increased at 61 months of cryopreservation (p < 0.05). GO and KEGG enrichment analyses of the DEGs revealed significant enrichment in the pilus assembly, metabolic process, MAPK signaling pathway, apoptosis, and P53 signaling pathway. Time-series expression profiling of the DEGs showed that consistently upregulated modules were also significantly enriched in signaling pathways associated with apoptosis. Four genes, scarb1, odf3, exoc8, and atp5f1d, were associated with mitochondria and flagella in a weighted correlation network analysis. These genes may play an important role in the response to sperm freezing. The experimental results show that long-term cryopreservation results in freezing damage to the giant grouper sperm. This study provides rich data for studies of the mechanism underlying frozen fish sperm damage as well as a technical reference and evaluation index for the long-term cryopreservation of fish sperm.
Sex-Specific Differences in Cytokine Production Capacity in Patients with Gout Compared to Controls
Authors: Badii Medeea , Gaal Orsolya I. , Hotea Ioana , Nica Valentin , Mirea Andreea M. , Mărginean Dragoş , HINT Consortium HINT Consortium , Pamfil Cristina , Rednic Simona , Popp Radu A. , Crişan Tania O. , Joosten Leo A. B.
Published: April 2024

Gout, an inflammatory disease orchestrated by interleukin-1β activation and release, is more prevalent in men. The clinical profiles of patients with gout report differences by sex. This study aims to investigate sex-specific cytokine profiles in circulation and in stimulated peripheral blood mononuclear cells (PBMCs) of patients with gout and controls. Participants included in the gout group met the criteria of the American College of Rheumatology/European League Against Rheumatism (ACR/EULAR). The control group included individuals with varying levels of serum urate and absence of gout. PBMCs were stimulated in vitro for 24 h with various TLR ligands. Cytokines were determined in culture supernatants and plasma. Plasma IL-1Ra and high-sensitivity C-reactive protein (hsCRP) were higher in men with gout compared to men without gout whereas no significant differences in circulating cytokines were observed in women. PBMCs of patients with gout showed higher cytokine production of IL-1β, IL-1Ra, and TNF-α following 24 h stimulation, predominantly observed in women. We identified sex-specific cytokine production in gout in response to in vitro stimulation. While men with gout had higher levels of circulating cytokines, stimulated PBMCs of women with gout show an enhanced capacity for cytokine production. These data may suggest potentially different regulatory mechanisms of inflammation in men and women with gout.
A Phenomenological Inquiry of the Shift to Virtual Care Delivery: Insights from Front-Line Primary Care Providers
Authors: Halas Gayle , Baldwin Alanna , LaBine Lisa , MacKay Kerri , Singer Alexander , Katz Alan
Published: April 2024

The rapid deployment of virtual primary care visits served as a first-line response to COVID-19 and can now be examined for insights, particularly as virtual care is playing an ongoing role in patient care and consultations. Input from primary care providers directly responsible for virtual care delivery is needed to inform policies and strategies for quality care and interactions. The overarching goal of this research study was to examine the use of virtual care as a mechanism for primary healthcare delivery. A phenomenological approach investigated the shift in primary care service delivery as experienced by primary care providers and initiated during the COVID-19 pandemic. Focus groups were conducted with primary care providers (n = 21) recruited through email, advertisements, and professional organizations, exploring how virtual care was delivered, the benefits and challenges, workflow considerations, and recommendations for future use. Integrating virtual care was performed with a great deal of autonomy as well as responsibility, and overwhelmingly depended on the telephone. Technology, communication, and workflow flexibility are three key operational aspects of virtual care and its delivery. Providers highlighted cross-cutting themes related to the dynamics of virtual care including balancing risk for quality care, physician work/life balance, efficiency, and patient benefits. Primary care providers felt that virtual care options allowed increased flexibility to attend to the needs of patients and manage their practice workload, and a few scenarios were shared for when virtual care might be best suited. However, they also recognized the need to balance in-person and virtual visits, which may require guidelines that support navigating various levels of care. Overall, virtual care was considered a good addition to the whole ‘care package’ but continued development and refinement is an expectation for optimizing and sustaining future use.
Validation of the Health Index in the Postoperative Period: Use of the Nursing Outcome Classification to Determine the Health Level
Authors: Herrero Jaén Sara , González Aguña Alexandra , Fernández Batalla Marta , Gonzalo de Diego Blanca , Sierra Ortega Andrea , Rocha Martínez María del Mar , Barchino Plata Roberto , Jiménez Rodríguez María Lourdes , Santamaría García José María
Published: April 2024

Background: The postoperative period is the recovery time after surgery and is defined as an individual process whose purpose is to return the person to the state of normality and integrity that they had prior to surgery. Aim: Demonstrate the modification of the level of health of people in the early postoperative period through the development and validation of the Health Index Instrument, which is built from the Nursing Outcomes Classification (NOC) standardized language. Design: The design used a mixed method, which involved a first phase of instrument development and a second phase of instrument validation. Methods: The methods was based on focus group techniques with text analysis techniques, internal validation with a group of care language experts, external validation with a group of clinical nursing experts and a clinical validation with quantitative and qualitative analysis. A panel of experts in Language of Care evaluated the (NOC) labels and their correlation with the 11 Health Variables to construct the instrument. The instrument developed was subjected to external validation with a panel of clinical nurse experts in post-anesthesia care. The clinical validation included a cross-sectional descriptive study in a postoperative unit. The final sample of the cross-sectional descriptive study was 139 cases. Results: Of the 89 NOCs proposed in the preliminary construction phase of the instrument, 36 passed through the first round. Of those 36 NOCs, 25 passed through to the second round with a review performance and 11 directly as approved. The total number of approved NOCs were 4. The results of the research show that there are changes in the global score of the health level and in each health variable. It is observed that there was a significant increase in the scores of the health variables at admission and discharge (p < 0.001). Conclusions: The results of the data analysis show that six groups present a similar pattern of evolution of the health variables. A correlation was found between the time of stay in the unit with the scores obtained in the health variables, the physical functioning, comfort status and the presence of symptoms being particularly significant.
Downside of Helping Professions: A Comparative Study of Health Indicators and Health Behaviour among Nurses and Early Childhood Educators
Authors: Csima Melinda , Podráczky Judit , Cseh Szabolcs , Sipos Dávid , Garai Sára , Fináncz Judit
Published: April 2024

The activities of health care workers and early childhood educators have received increased attention both in lay public discourse and in scientific discourse. These professional groups play a significant role in shaping the health behaviours of those they interact with; thus, understanding the patterns they convey is of paramount importance. The aim of our study is a comparative analysis of health conditions and health behaviours of professionals working in Hungarian early childhood education and nurses working in the healthcare system (n = 1591). We carried out our quantitative, cross-sectional research using convenience sampling among healthcare professionals working in nursing job positions (n = 581) and as early childhood educators (n = 1010), in south-west Hungary. Diagnosed chronic illnesses affect early childhood educators at a significantly higher rate (p < 0.05): the prevalence of musculoskeletal disorders is particularly high among them, as a result of which they reported a significant degree of physical limitation in relation to work. In the context of mental health, comparing the professional groups, nurses’ indicators were significantly (p < 0.001) more unfavourable in all examined dimensions. Moreover, the comparison in terms of educational attainment directed attention to the worse indicators of non-graduates. In this context, early childhood educators are less affected by all three dimensions of burnout (p < 0.001). As for health behaviour, the smoking habits of nurses are more unfavourable (p < 0.05). Regarding screening tests, participation in cytological testing was significantly higher among nurses, whereas early childhood educators showed increased participation in mammography (p < 0.001). Our findings draw attention to the fact that early childhood educators are primarily affected by chronic musculoskeletal disorders, while healthcare workers are more affected by problems related to mental health. Mental well-being can be further endangered by the fact that both professional groups perceive low social appreciation for the work they carry out.
Perinatal Outcomes and Level of Labour Difficulty in Deliveries with Right and Left Foetal Position—A Preliminary Study
Authors: Witkiewicz Magdalena , Baranowska Barbara , Węgrzynowska Maria , Kiersnowska Iwona , Karzel Katarzyna , Bączek Grażyna , Sys Dorota , Scholz Anna , Crowther Susan , Teliga-Czajkowska Justyna , Tataj-Puzyna Urszula
Published: April 2024

Background: Many studies have shown the negative influence of the foetus’s occiput posterior position during birth on the final perinatal outcome. This study aims to add to the discussion on the impact of foetus positioning on the course of labour and subjective assessment of the level of labour difficulty. Methods: The cross-sectional study took place from February 2020 to September 2021, and consisted of filling out observation forms and the assessment by the midwives and women of the level of labour difficulty. This study is based on the observation of 152 labours in low-risk women. Findings: When compared to left foetal positioning, labours in which the foetus was in the right position were longer and more frequently failed to progress (in 11.3% vs. 37.5%), and epidural was more frequently administrated (in 30.4% vs. 52.7%). Both women and midwives subjectively evaluated deliveries with a foetus in the right position as more difficult. Conclusions: The right positioning of the foetus was related to greater labour difficulty and worse perinatal outcomes. The position of the foetus’ head in relation to the pelvis should be considered as an indicator of the difficulty of labour and a support plan for the woman should be offered accordingly.
Social Media Campaigns: A Game Changer for the Prevention of Breast Cancer in Romania
Authors: Todor Raluca Dania , Brătucu Gabriel , Candrea Adina Nicoleta , Strempel Christian Gabriel , Anastasiu Costin Vlad
Published: April 2024

Social media can be used to raise awareness about health issues, especially concerning the importance of periodical screening. The present study aims to identify the role of social media awareness campaigns in the early detection of breast cancer, with a focus on Romania, a country with a high female mortality due to this disease. The research is performed using a survey, based on an online questionnaire, which was self-administered by the respondents included in two samples selected from a Facebook community of Romanian women. Sample A was composed of 1945 women who were not exposed to periodic campaigns regarding the importance of screening for the prevention and early detection of this type of disease, and Sample B was composed of 289 women who were exposed to such annual campaigns within the last 5 years. The results outline several differences among respondents from the two samples regarding their awareness of prevention necessity, as well as the frequency and chosen methods for breast cancer screening. The findings hold important implications for public authorities, which must intensify their efforts in raising women’s awareness regarding the importance of screening for the early detection and prevention of breast cancer.
The 8:1:1 Supplementation of Branched-Chain Amino Acids in High-Intensity Training: A Case Study of the Protective Effect on Rhabdomyolysis
Authors: Vicario-Merino Angel , Soriano Marcos A. , Jiménez-Ormeño Ester , Ruiz-Moreno Carlos , Gallo-Salazar Cesar , Areces-Corcuera Francisco
Published: April 2024

Introduction: The increasing prevalence of high-intensity sports activities, notably the burgeoning popularity of CrossFit, underscores the contemporary significance of such physical pursuits. The discernible protective impact of branched-chain amino acids on muscle fatigue and injuries is emerging as a noteworthy area of investigation. Within the realm of sports, integrating BCAA supplementation into dietary practices holds promise for aiding athletes in their recovery, particularly in mitigating Delayed-Onset Muscle Soreness. Methodology: This study adopted an experimental pilot design with repeated measures, employing a controlled and randomized approach through double-blind procedures. The participant engaged in high-intensity activity, specifically the CrossFit Karen® test, which entailed executing 150 wall ball throws (9 kg) to a height of 3 m. The trial incorporated three randomized supplementation conditions: BCAAs in an 8:1:1 ratio or a 2:1:1 ratio or a placebo condition. The participant consumed 15 g daily for 7 days, commencing 72 h prior to the initial blood sample and the first Karen® test. Results: In this study, BCAA supplementation at an 8:1:1 ratio demonstrated a discernible protective effect against muscular damage, as evidenced by creatine kinase values and ratings of perceived exertion.
Traditional Foods as a Way to Preserve the Genetic Diversity of the Grapevine (Vitis vinifera) in Tunisia
Authors: Saddoud Debbabi Olfa , Ben Slimane Mounira , Ben Hadj Alouane Rym Bouhlal , Montemurro Cinzia , Snoussi Hager , Miazzi Monica Marilena
Published: April 2024

Tunisia has a long tradition of viticulture, and the grapevine is used for a variety of traditional foods. The country has many plant-based dishes, which may have different compositions and names depending on the region of origin. Unfortunately, over the years, traditional preparations are no longer as popular as they used to be, due to both changing lifestyles and the introduction of new crops and foods. For example, the use of grapevine in traditional dishes has declined as traditions have been lost and habits have changed in favor of new, ready-to-eat foods. The close link between grapevine, culture and territory requires an appropriate evaluation and characterization of traditional foods in order to enhance and extend their use, ensure their sustainability and preserve the varieties used to produce them. This review analyzes the use of local grapevine varieties in different traditional dishes as a means to preserve an invaluable natural and cultural resource and heritage.
Evaluating Best Management Practice Efficacy Based on Seasonal Variability and Spatial Scales
Authors: Venishetty Vivek , Parajuli Prem B. , To Filip , Nepal Dipesh , Baker Beth , Gude Veera Gnaneswar
Published: April 2024

Implementing best management practices (BMPs) has proven to be an efficient method for reducing non-point source (NPS) pollutants. Agricultural NPS pollution is considered to be a major contributor to water quality impairment. This study aims to assess the variation in hydrologic and water quality outputs at field and watershed scales when BMPs are implemented using modeling approaches. The Yazoo River Watershed (YRW) is the largest watershed basin in the state of Mississippi with approximately 50% agricultural land. Runoff generated from agricultural areas carries sediments and nutrients. The Merigold watershed (MW) is a sub-basin of the YRW and a field-scale watershed with most of the land use being agriculture. It is essential to quantify the streamflow, sediment, total nitrogen (TN), and total phosphorus (TP) when BMPs are implemented. BMPs such as vegetative filter strips (VFS) and cover crops (CC) were tested in this study. The Soil and Water Assessment Tool (SWAT) model was applied to quantify the watershed’s hydrologic and water quality outputs. SWAT model accuracy assessment was performed by calibration and validation process using the Nash and Sutcliffe Efficiency Index (NSE). Model performance was satisfactory for monthly streamflow, with NSE values in the range of 0.62 to 0.81, and for daily sediments, TN, and TP load estimation, with NSE values of 0.21, 0.20, and 0.47, respectively. CC was planted after harvesting the main crop. Therefore, it is essential to quantify the seasonal reduction in pollutants. Water quality was improved after BMP implementation, and an overall decrease in streamflow, sediment, TN, and TP loads was observed for both MW and YRW during dry and wet seasons. Previous studies regarding seasonal assessments with CC implementation in the MW and YRW were limited. Therefore, the results from this study could be a unique addition to the scientific literature.
A Novel Address-Matching Framework Based on Region Proposal
Authors: Quan Yizhuo , Chang Yuanfei , Liang Linlin , Qiao Yanyou , Wang Chengbo
Published: April 2024

Geocoding is a fundamental component of geographic information science that plays a crucial role in various geographical studies and applications involving text data. Current mainstream geocoding methods fall into two categories: geodesic-grid prediction and address matching. However, the geodesic-grid-prediction method’s localization accuracy is hindered by the density of grid partitioning, struggling to strike a balance between prediction accuracy and grid density. Address-matching methods mainly focus on the semantics of query text. However, they tend to ignore keyword information that can be used to distinguish candidates and introduce potential interference, which reduces matching accuracy. Inspired by the human map-usage process, we propose a two-stage address-matching approach that integrates geodesic-grid prediction and text-matching models. Initially, a multi-level text-classification model is used to generate a retrieval region proposal for an input query text. Subsequently, we search for the most relevant point of interest (POI) within the region-proposal area using a semantics-based text-retrieval model. We evaluated the proposed method using POI data from the Beijing Chaoyang District. The experimental results indicate that the proposed method provides high address-matching accuracy, increasing Recall@1 by 0.55 to 1.56 percentage points and MRR@5 by 0.54 to 1.68 percentage points.
Oral Microbially-Induced Small Extracellular Vesicles Cross the Blood–Brain Barrier
Authors: Elashiry Mahmoud , Carroll Angelica , Yuan Jessie , Liu Yutao , Hamrick Mark , Cutler Christopher W. , Wang Qin , Elsayed Ranya
Published: April 2024

Porphyromonas gingivalis (Pg) and its gingipain proteases contribute to Alzheimer’s disease (AD) pathogenesis through yet unclear mechanisms. Cellular secretion of small extracellular vesicles or exosomes (EXO) increases with aging as part of the senescence-associated secretory phenotype (SASP). We have shown that EXO isolated from Pg-infected dendritic cells contain gingipains and other Pg antigens and transmit senescence to bystander gingival cells, inducing alveolar bone loss in mice in vivo. Here, EXO were isolated from the gingiva of mice and humans with/without periodontitis (PD) to determine their ability to penetrate the blood–brain barrier (BBB) in vitro and in vivo. PD was induced by Pg oral gavage for 6 weeks in C57B6 mice. EXO isolated from the gingiva or brain of donor Pg-infected (PD EXO) or control animals (Con EXO) were characterized by NTA, Western blot, and TEM. Gingival PD EXO or Con EXO were labeled and injected into the gingiva of uninfected WT mouse model. EXO biodistribution in brains was tracked by an in vivo imaging system (IVIS) and confocal microscopy. The effect of human PD EXO on BBB integrity and permeability was examined using TEER and FITC dextran assays in a human in vitro 3D model of the BBB. Pg antigens (RGP and Mfa-1) were detected in EXO derived from gingival and brain tissues of donor Pg-infected mice. Orally injected PD EXO from donor mice penetrated the brains of recipient uninfected mice and colocalized with hippocampal microglial cells. IL-1β and IL-6 were expressed in human PD EXO and not in Con EXO. Human PD EXO promoted BBB permeability and penetrated the BBB in vitro. This is the first demonstration that microbial-induced EXO in the oral cavity can disseminate, cross the BBB, and may contribute to AD pathogenesis.
Mechanisms Involved in the Link between Depression, Antidepressant Treatment, and Associated Weight Change
Authors: Kukucka Tomas , Ferencova Nikola , Visnovcova Zuzana , Ondrejka Igor , Hrtanek Igor , Kovacova Veronika , Macejova Andrea , Mlyncekova Zuzana , Tonhajzerova Ingrid
Published: April 2024

Major depressive disorder is a severe mood disorder associated with a marked decrease in quality of life and social functioning, accompanied by a risk of suicidal behavior. Therefore, seeking out and adhering to effective treatment is of great personal and society-wide importance. Weight changes associated with antidepressant therapy are often cited as the reason for treatment withdrawal and thus are an important topic of interest. There indeed exists a significant mechanistic overlap between depression, antidepressant treatment, and the regulation of appetite and body weight. The suggested pathomechanisms include the abnormal functioning of the homeostatic (mostly humoral) and hedonic (mostly dopaminergic) circuits of appetite regulation, as well as causing neuromorphological and neurophysiological changes underlying the development of depressive disorder. However, this issue is still extensively discussed. This review aims to summarize mechanisms linked to depression and antidepressant therapy in the context of weight change.
Hollow Mesoporous Silica Nanoparticles as a New Nanoscale Resistance Inducer for Fusarium Wilt Control: Size Effects and Mechanism of Action
Authors: Ding Chaopu , Zhang Yunfei , Chen Chongbin , Wang Junfang , Qin Mingda , Gu Yu , Zhang Shujing , Wang Lanying , Luo Yanping
Published: April 2024

In agriculture, soil-borne fungal pathogens, especially Fusarium oxysporum strains, are posing a serious threat to efforts to achieve global food security. In the search for safer agrochemicals, silica nanoparticles (SiO2NPs) have recently been proposed as a new tool to alleviate pathogen damage including Fusarium wilt. Hollow mesoporous silica nanoparticles (HMSNs), a unique class of SiO2NPs, have been widely accepted as desirable carriers for pesticides. However, their roles in enhancing disease resistance in plants and the specific mechanism remain unknown. In this study, three sizes of HMSNs (19, 96, and 406 nm as HMSNs-19, HMSNs-96, and HMSNs-406, respectively) were synthesized and characterized to determine their effects on seed germination, seedling growth, and Fusarium oxysporum f. sp. phaseoli (FOP) suppression. The three HMSNs exhibited no side effects on cowpea seed germination and seedling growth at concentrations ranging from 100 to 1500 mg/L. The inhibitory effects of the three HMSNs on FOP mycelial growth were very weak, showing inhibition ratios of less than 20% even at 2000 mg/L. Foliar application of HMSNs, however, was demonstrated to reduce the FOP severity in cowpea roots in a size- and concentration-dependent manner. The three HMSNs at a low concentration of 100 mg/L, as well as HMSNs-19 at a high concentration of 1000 mg/L, were observed to have little effect on alleviating the disease incidence. HMSNs-406 were most effective at a concentration of 1000 mg/L, showing an up to 40.00% decline in the disease severity with significant growth-promoting effects on cowpea plants. Moreover, foliar application of HMSNs-406 (1000 mg/L) increased the salicylic acid (SA) content in cowpea roots by 4.3-fold, as well as the expression levels of SA marker genes of PR-1 (by 1.97-fold) and PR-5 (by 9.38-fold), and its receptor gene of NPR-1 (by 1.62-fold), as compared with the FOP infected control plants. Meanwhile, another resistance-related gene of PAL was also upregulated by 8.54-fold. Three defense-responsive enzymes of POD, PAL, and PPO were also involved in the HMSNs-enhanced disease resistance in cowpea roots, with varying degrees of reduction in activity. These results provide substantial evidence that HMSNs exert their Fusarium wilt suppression in cowpea plants by activating SA-dependent SAR (systemic acquired resistance) responses rather than directly suppressing FOP growth. Overall, for the first time, our results indicate a new role of HMSNs as a potent resistance inducer to serve as a low-cost, highly efficient, safe and sustainable alternative for plant disease protection.
Characterization of Alternative Splicing in High-Risk Wilms’ Tumors
Authors: Trink Yaron , Urbach Achia , Dekel Benjamin , Hohenstein Peter , Goldberger Jacob , Kalisky Tomer
Published: April 2024

The significant heterogeneity of Wilms’ tumors between different patients is thought to arise from genetic and epigenetic distortions that occur during various stages of fetal kidney development in a way that is poorly understood. To address this, we characterized the heterogeneity of alternative mRNA splicing in Wilms’ tumors using a publicly available RNAseq dataset of high-risk Wilms’ tumors and normal kidney samples. Through Pareto task inference and cell deconvolution, we found that the tumors and normal kidney samples are organized according to progressive stages of kidney development within a triangle-shaped region in latent space, whose vertices, or “archetypes”, resemble the cap mesenchyme, the nephrogenic stroma, and epithelial tubular structures of the fetal kidney. We identified a set of genes that are alternatively spliced between tumors located in different regions of latent space and found that many of these genes are associated with the epithelial-to-mesenchymal transition (EMT) and muscle development. Using motif enrichment analysis, we identified putative splicing regulators, some of which are associated with kidney development. Our findings provide new insights into the etiology of Wilms’ tumors and suggest that specific splicing mechanisms in early stages of development may contribute to tumor development in different patients.
High-Resolution Cryo-Electron Microscopy Structure Determination of Haemophilus influenzae Tellurite-Resistance Protein A via 200 kV Transmission Electron Microscopy
Authors: Tran Nhi L. , Senko Skerdi , Lucier Kyle W. , Farwell Ashlyn C. , Silva Sabrina M. , Dip Phat V. , Poweleit Nicole , Scapin Giovanna , Catalano Claudio
Published: April 2024

Membrane proteins constitute about 20% of the human proteome and play crucial roles in cellular functions. However, a complete understanding of their structure and function is limited by their hydrophobic nature, which poses significant challenges in purification and stabilization. Detergents, essential in the isolation process, risk destabilizing or altering the proteins’ native conformations, thus affecting stability and functionality. This study leverages single-particle cryo-electron microscopy to elucidate the structural nuances of membrane proteins, focusing on the SLAC1 bacterial homolog from Haemophilus influenzae (HiTehA) purified with diverse detergents, including n-dodecyl β-D-maltopyranoside (DDM), glycodiosgenin (GDN), β-D-octyl-glucoside (OG), and lauryl maltose neopentyl glycol (LMNG). This research not only contributes to the understanding of membrane protein structures but also addresses detergent effects on protein purification. By showcasing that the overall structural integrity of the channel is preserved, our study underscores the intricate interplay between proteins and detergents, offering insightful implications for drug design and membrane biology.
Bactericidal Chitosan Derivatives and Their Superabsorbent Blends with ĸ-Carrageenan
Authors: Lewicka Kamila , Smola-Dmochowska Anna , Śmigiel-Gac Natalia , Kaczmarczyk Bożena , Janeczek Henryk , Barczyńska-Felusiak Renata , Szymanek Izabela , Rychter Piotr , Dobrzyński Piotr
Published: April 2024

The aim of this work is research dedicated to the search for new bactericidal systems for use in cosmetic formulations, dermocosmetics, or the production of wound dressings. Over the last two decades, chitosan, due to its special biological activity, has become a highly indispensable biopolymer with very wide application possibilities. Reports in the literature on the antibacterial effects of chitosan are very diverse, but our research has shown that they can be successfully improved through chemical modification. Therefore, in this study, results on the synthesis of new chitosan-based Schiff bases, dCsSB-SFD and dCsSB-PCA, are obtained using two aldehydes: sodium 4-formylbenzene-1,3-disulfonate (SFD) and 2-pyridine carboxaldehyde (PCA), respectively. Chitosan derivatives synthesized in this way demonstrate stronger antimicrobial activity. Carrying out the procedure of grafting chitosan with a caproyl chain allowed obtaining compatible blends of chitosan derivatives with κ-carrageenan, which are stable hydrogels with a high swelling coefficient. Furthermore, the covalently bounded poly(ε-caprolactone) (PCL) chain improved the solubility of obtained polymers in organic solvents. In this respect, the Schiff base-containing polymers obtained in this study, with special hydrogel and antimicrobial properties, are very promising materials for potential use as a controlled-release formulation of both hydrophilic and hydrophobic drugs in cosmetic products for skin health.
Using Targeted Metabolomics to Unravel Phenolic Metabolites of Plant Origin in Animal Milk
Authors: Agulló Vicente , Favari Claudia , Pilla Niccolò , Bresciani Letizia , Tomás-Barberán Francisco A. , Crozier Alan , Del Rio Daniele , Mena Pedro
Published: April 2024

Milk holds a high nutritional value and is associated with diverse health benefits. The understanding of its composition of (poly)phenolic metabolites is limited, which necessitates a comprehensive evaluation of the subject. This study aimed at analyzing the (poly)phenolic profile of commercial milk samples from cows and goats and investigating their sterilization treatments, fat content, and lactose content. Fingerprinting of phenolic metabolites was achieved by using ultra-high-performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS/MS). Two hundred and three potential microbial and phase II metabolites of the main dietary (poly)phenols were targeted. Twenty-five metabolites were identified, revealing a diverse array of phenolic metabolites in milk, including isoflavones and their microbial catabolites equol and O-desmethylangolensin, phenyl-γ-valerolactones (flavan-3-ol microbial catabolites), enterolignans, urolithins (ellagitannin microbial catabolites), benzene diols, and hippuric acid derivates. Goat’s milk contained higher concentrations of these metabolites than cow’s milk, while the sterilization process and milk composition (fat and lactose content) had minimal impact on the metabolite profiles. Thus, the consumption of goat’s milk might serve as a potential means to supplement bioactive phenolic metabolites, especially in individuals with limited production capacity. However, further research is needed to elucidate the potential health effects of milk-derived phenolics.
NLRP3 Inflammasome in Acute and Chronic Liver Diseases
Authors: Sayaf Katia , Battistella Sara , Russo Francesco Paolo
Published: April 2024

NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) is an intracellular complex that upon external stimuli or contact with specific ligands, recruits other components, forming the NLRP3 inflammasome. The NLRP3 inflammasome mainly mediates pyroptosis, a highly inflammatory mode of regulated cell death, as well as IL-18 and IL-1β production. Acute and chronic liver diseases are characterized by a massive influx of pro-inflammatory stimuli enriched in reactive oxygen species (ROS) and damage-associated molecular patterns (DAMPs) that promote the assemblage and activation of the NLRP3 inflammasome. As the major cause of inflammatory cytokine storm, the NLRP3 inflammasome exacerbates liver diseases, even though it might exert protective effects in regards to hepatitis C and B virus infection (HCV and HBV). Here, we summarize the current knowledge concerning NLRP3 inflammasome function in both acute and chronic liver disease and in the post liver transplant setting, focusing on the molecular mechanisms involved in NLRP3 activity.