bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Early Breast Cancer Recurrence Mimicking BIA-ALCL in a Patient With Multiple Breast Procedures
Authors: Materazzo Marco , Vanni Gianluca , Pellicciaro Marco , Anemona Lucia , Servadei Francesca , Giacobbi Erika , Farinaccio Andrea , Pistolese Chiara Adriana , Perretta Tommaso , Chiocchi Marcello , Bruno Valentina , Tacconi Federico , Sadri Amir , De Majo Adriano , Di Pasquali Camilla , Meucci Rosaria , Santori Francesca , Cotesta Maria , Buonomo Oreste Claudio
Published: March 2021

Breast reconstruction plays a fundamental role in the therapeutic process of breast cancer treatment and breast implants represents the leading breast reconstruction strategy. Breast Implant Associated-Anaplastic Large Cell Lymphoma (BIA-ALCL), locoregional recurrence in the skin flap, and skin flap necrosis are well-known complications following mastectomy and immediate breast reconstruction (IBR). We report a case of locoregional cancer recurrence in the mastectomy flap mimicking BIA-ALCL, in a patient who underwent 6 breast procedures in four facilities across 15 years including immediate breast reconstruction with macrotextured breast implants. Despite the rate and onset of the disease, clinicians should be aware of BIA-ALCL. Due to the risk of false negative results of fine needle aspiration, clinical suspicion of BIA-ALCL should drive clinicians' choices, aside from cytological results. In the present case, surgical capsulectomy of the abnormal periprosthesic tissue revealed locoregional recurrence.
Case Report: Surgical Management of Painful Manubriosternal Pseudoarthrosis
Authors: Bar-Haim Ronit , Shtarker Haim , Biswas Seema , Waksman Igor , Altman Edward
Published: March 2021

A 31-year-old male amateur bodybuilder presented with a 2-year history of chronic pain over the sternum and a clicking sensation in the chest wall on movement. Ultrasound, computed tomography (CT), and magnetic resonance imaging (MRI) showed no cause for his symptoms. Dynamic ultrasound scan performed at a specialist sports center revealed pseudoarthrosis of the manubriosternal joint (MSJ). After a period of conservative management (rest and analgesia), he failed to improve and underwent debridement and fusion of the MSJ with plates and screws. At follow-up 23 months later, he remains pain-free and has returned to weight lifting and bodybuilding.
Wood Vinegar as a Complex Growth Regulator Promotes the Growth, Yield, and Quality of Rapeseed
Authors: Zhu Kunmiao , Gu Sicheng , Liu Jiahuan , Luo Tao , Khan Zaid , Zhang Kangkang , Hu Liyong
Published: March 2021

Wood vinegar is formed by the condensation of smoke produced during the production of biochar. It mainly contains acetic acid, butyric acid, catechol, and phenol. Wood vinegar has a compound effect of promoting crop growth similar to plant growth regulators and is environmentally friendly. Moreover, it can enhance the biological and abiotic resistance of crops. In this study, foliar spraying was carried out systematically in the field with the hybrid variety of Huayouza 9 for two years to study the effects of wood vinegar and its compounds on the growth of rapeseed (Brassica napus L.). We applied four treatments with tap water as a control (CK), namely wood vinegar diluted 400-fold (M), M mixed with gibberellin (T1), M mixed with sodium D-gluconate (T2), and M mixed with melatonin (T3). They were sprayed in the seedling stage and overwintering stage, respectively. The results showed that the seed yield, the leaf area index, and the number of pods per plant of rapeseed treated with M increased by an average of 9.58%, 23.45%, and 23.80% in two years as compared to the CK, respectively. Compared with M, the seed yield of rapeseed treated with T1, T2, and T3 increased by an average of 7.88%, 6.90%, and 1.32% in two years, respectively. The treatments also improved the quality of rapeseed. In particular, the glucosinolate content of rapeseed treated with T2 and T3 decreased by an average of 12.83% and 6.72% in two years compared to the CK, respectively. The four treatments selected in the current study improved the resistance of rapeseed at the low temperature of 2–6 °C by increasing the activity of superoxide dismutase and proline and soluble protein contents, as compared to the CK. Besides, all treatments containing M reduced the incidence of Sclerotinia sclerotiorum and Peronospora parasitica (downy mildew) in rapeseed. More specifically, the T3 treatment significantly decreased the infection rate of these two diseases mentioned above by an average of 17.33% and 12.14% in two years compared to the CK, respectively. Therefore, the study and application of wood vinegar due to its compound effects on crop growth and yield is of great importance to sustainable agriculture, crop ecology, and environmental protection.
Peripheral Modulators of the Central Fatigue Development and Their Relationship with Athletic Performance in Jumper Horses
Authors: Arfuso Francesca , Giannetto Claudia , Giudice Elisabetta , Fazio Francesco , Panzera Michele , Piccione Giuseppe
Published: March 2021

The current study aimed to investigate whether peripheral modulators of serotoninergic function and neurohumoral factors’ changes in athletic horses during an official jumping competition, and to evaluate their relationship with the physical performance of competing horses. From 7 Italian Saddle mares (6–9 years; mean body weight 440 ± 15 kg), performing the same standardized warm-up and jumping course during an official class, heart rate (HR) was monitored throughout the competition. Rectal temperature (RT) measurement, blood lactate and glucose concentration, serum tryptophan, leucine, valine, the tryptophan/branched-chain amino-acids ratio (Try/BCAAs), dopamine, prolactin, and non-esterified fatty acids (NEFAs) were assessed before the exercise event (T0), at the end of the competition stage (5 min ± 10 s following the cessation of the exercise, TPOST5), and 30 min after the end of competition (TPOST30). Highest HR values were recorded during the course and at the outbound (p < 0.0001); blood lactate concentration and RT increased after exercise with respect to the rest condition (p < 0.0001). Lower leucine and valine levels (p < 0.01), and higher tryptophan, Try/BCAAs ratio, and NEFAs values were found at TPOST5 and TPOST30 with respect to T0 (p < 0.0001). A higher prolactin concentration was found at TPOST5 and TPOST30 compared to T0 (p < 0.0001), whereas dopamine showed decreased values after exercise compared to rest (p < 0.0001). Statistically significant correlations among the peripheral indices of serotoninergic function, neurohumoral factors, and athletic performance parameters were found throughout the monitoring period. The findings provide indirect evidence that the serotoninergic system may be involved in fatigue during jumper exercise under a stressful situation, such as competition, in which, in addition to physical effort, athletic horses exhibit more passive behavior.
PBP4 Is Likely Involved in Cell Division of the Longitudinally Dividing Bacterium Candidatus Thiosymbion Oneisti
Authors: Wang Jinglan , Alvarez Laura , Bulgheresi Silvia , Cava Felipe , den Blaauwen Tanneke
Published: March 2021

Peptidoglycan (PG) is essential for bacterial survival and maintaining cell shape. The rod-shaped model bacterium Escherichia coli has a set of seven endopeptidases that remodel the PG during cell growth. The gamma proteobacterium Candidatus Thiosymbion oneisti is also rod-shaped and attaches to the cuticle of its nematode host by one pole. It widens and divides by longitudinal fission using the canonical proteins MreB and FtsZ. The PG layer of Ca. T. oneisti has an unusually high peptide cross-linkage of 67% but relatively short glycan chains with an average length of 12 disaccharides. Curiously, it has only two predicted endopeptidases, MepA and PBP4. Cellular localization of symbiont PBP4 by fluorescently labeled antibodies reveals its polar localization and its accumulation at the constriction sites, suggesting that PBP4 is involved in PG biosynthesis during septum formation. Isolated symbiont PBP4 protein shows a different selectivity for β-lactams compared to its homologue from E. coli. Bocillin-FL binding by PBP4 is activated by some β-lactams, suggesting the presence of an allosteric binding site. Overall, our data point to a role of PBP4 in PG cleavage during the longitudinal cell division and to a PG that might have been adapted to the symbiotic lifestyle.
The Role of Antibiotic Resistant A. baumannii in the Pathogenesis of Urinary Tract Infection and the Potential of Its Treatment with the Use of Bacteriophage Therapy
Authors: Bagińska Natalia , Cieślik Martyna , Górski Andrzej , Jończyk-Matysiak Ewa
Published: March 2021

Acinetobacter baumannii are bacteria that belong to the critical priority group due to their carbapenems and third generation cephalosporins resistance, which are last-chance antibiotics. The growing multi-drug resistance and the ability of these bacteria to form biofilms makes it difficult to treat infections caused by this species, which often affects people with immunodeficiency or intensive care unit patients. In addition, most of the infections are associated with catheterization of patients. These bacteria are causative agents, inter alia, of urinary tract infections (UTI) which can cause serious medical and social problems, because of treatment difficulties as well as the possibility of recurrence and thus severely decrease patients’ quality of life. Therefore, a promising alternative to standard antibiotic therapy can be bacteriophage therapy, which will generate lower costs and will be safer for the treated patients and has real potential to be much more effective. The aim of the review is to outline the important role of drug-resistant A. baumannii in the pathogenesis of UTI and highlight the potential for fighting these infections with bacteriophage therapy. Further studies on the use of bacteriophages in the treatment of UTIs in animal models may lead to the use of bacteriophage therapy in human urinary tract infections caused by A. baumannii in the future.
Energy Investment Risk Assessment for Nations along China’s Belt & Road Initiative: A Deep Learning Method
Authors: Liang Panning , Yu Mingyang , Jiang Lincheng
Published: March 2021

In 2013, China proposed the “Belt & Road Initiative” which aims to invest the “Belt & Road” countries so as to help them develop their infrastructure and economy. China consumes the largest part of fossil energy of the whole world, so it is China’s priority to consider its energy supplying security. Therefore, it becomes urgent for China to invest the “Belt & Road” countries’ energy facilities. There comes a question: how to evaluate the overseas energy investment risk? To answer this question, this paper proposes a deep learning method to assess such risk of the 50 “Belt & Road” countries. Specifically, this paper first proposes an indicator system in which 6 main factors are separated into 36 sub-factors. This paper makes use of hierarchical convolution neural networks (CNN) to encode the historical statistics. The hierarchical structure could help CNN handle the long historical statistics more effectively and efficiently. Afterward, this paper leverages the self-attention layer to calculate the weights of each sub-factor. It could be observed that the resource potential is the most important indicator, while “years of China’s diplomatic relations” is the most important sub-indicator. Finally, we use a conditional random field (CRF) layer and softmax layer to compute the assessment scores of each country. Based on the experimental results, this paper suggests Russia, United Arab Emirates (UAE), Malaysia, Saudi Arabia, Pakistan, Indonesia, and Kazakhstan to be China’s most reliable choices for energy investment.
Intelligent Exploration Approaches Based on Utility Functions Optimization for Multi-Agent Environment Applications
Authors: Oñate-López José , Navarro Loraine , Quintero M. Christian G. , Pardo Mauricio
Published: March 2021

In this work, the problem of exploring an unknown environment with a team of agents and search different targets on it is considered. The key problem to be solved in multiple agents is choosing appropriate target points for the individual agents to simultaneously explore different regions of the environment. An intelligent approach is presented to coordinate several agents using a market-based model to identify the appropriate task for each agent. It is proposed to compare the fitting of the market utility function using neural networks and optimize this function using genetic algorithms to avoid heavy computation in the Non-Polynomial (NP: nondeterministic polynomial time) path-planning problem. An indoor environment inspires the proposed approach with homogeneous physical agents, and its performance is tested in simulations. The results show that the proposed approach allocates agents effectively to the environment and enables them to carry out their mission quickly.
Mixed Reality: A Reconsideration Based on Mixed Objects and Geospatial Modalities
Authors: Evangelidis Konstantinos , Papadopoulos Theofilos , Sylaiou Stella
Published: March 2021

We put forward a conceptualization of Mixed Reality as a blend of digital objects with real ones that coexist and interact with each other and they are also spatially referenced so that they are properly perceived in space by an observer that could potentially be at any position any time. In accordance with this statement, we have adopted the concept of a Mixed Object which is composed of a set of physical properties linked with a set of digital ones. In our case, the physical properties are acquired by employing geospatial technologies such as photogrammetry, laser scanning, unmanned aerial vehicles and positioning systems and are further processed in order to be visually displayed by utilizing Geographical Information Systems and Geovisualization frameworks in combination with traditional image processing techniques. We show that the Mixed Object approach is in conformance with Microsoft’s approach on Mixed Reality as the common space between humans, computers, and the environment and we further incorporate in these the Geospatial Linking Modalities. We finally provide an affordable MR experience as a proof of concept, by utilizing a smartphone for capturing and visualizing the environment, a visual tag for positioning purposes and freely available photogrammetrically mapped content and virtual objects to form a digital scene blended with the real environment.
Biomechanical Consequences of Tibial Insert Thickness after Total Knee Arthroplasty: A Musculoskeletal Simulation Study
Authors: Tzanetis Periklis , Marra Marco A. , Fluit René , Koopman Bart , Verdonschot Nico
Published: March 2021

The thickness of the tibial polyethylene (PE) insert is a critical parameter to ensure optimal soft-tissue balancing in the intraoperative decision-making procedure of total knee arthroplasty (TKA). However, there is a paucity of information about the kinetic response to PE insert thickness variations in the tibiofemoral (TF) joint, and subsequently, the secondary effects on the patellofemoral (PF) biomechanics. Therefore, the purpose of this study was to investigate the influence of varying PE insert thickness on the ligament and TF compressive forces, as well as on the PF forces and kinematics, after a cruciate-retaining TKA. A previous patient-specific musculoskeletal model of TKA was adapted to simulate a chair-rising motion in which PE insert thickness was varied with 2 mm increments or decrements compared to the reference case (9 mm), from 5 mm up to 13 mm. Greater PE insert thickness resulted in higher ligament forces and concurrently increased the TF compressive force by 21% (13 mm), but slightly unloaded the PF joint with 7% (13 mm) while shifting the patella distally in the trochlear groove, compared to the reference case. Thinner PE inserts showed an opposite trend. Our findings suggest that the optimal PE insert thickness selection is a trade-off between the kinetic outcomes of the TF and PF joints.
A Complete and Fast Analysis Procedure for Three-Phase Induction Motors Using Finite Element, Considering Skewing and Iron Losses
Authors: Carbonieri Matteo , Bianchi Nicola
Published: March 2021

This paper deals with a complete finite-element analysis procedure for squirrel cage induction motors, including the presence of skewing and the iron losses evaluation. The machine is analyzed performing only magneto-static finite element analyses. Saturation phenomena are carefully considered in any operating condition, avoiding long time-stepping analyses. The synergy between analytical and finite element model leads to a rapid and precise estimation of the rotor induced current, saving computational time. Furthermore, the procedure proposed in this paper allows the motor performance to be directly derived, without the preliminary knowledge of the machine equivalent circuit. In order to complete the analysis, skewing effect is included, using the 2-D multi-slice technique, based on static simulations. Experimental tests are carried out and reported in order to verify analysis results.
Heart Diseases Diagnose via Mobile Application
Authors: Güven Mesut , Hardalaç Fırat , Özışık Kanat , Tuna Funda
Published: March 2021

One of the oldest and most common methods of diagnosing heart abnormalities is auscultation. Even for experienced medical doctors, it is not an easy task to detect abnormal patterns in the heart sounds. Most digital stethoscopes are now capable of recording and transferring heart sounds. Moreover, it is proven that auscultation records can be classified as healthy or unhealthy via artificial intelligence techniques. In this work, an artificial intelligence-powered mobile application that works in a connectionless fashion is presented. According to the clinical experiments, the mobile application can detect heart abnormalities with approximately 92% accuracy, which is comparable to if not better than humans since only a small number of well-trained cardiologists can analyze auscultation records better than artificial intelligence. Using the diagnostic ability of artificial intelligence in a mobile application would change the classical way of auscultation for heart disease diagnosis.
Investigation of Thermal Effects of Radiofrequency Ablation Mediated with Iron Oxide Nanoparticles Dispersed in Agarose and Chitosan Solvents
Authors: Ashikbayeva Zhannat , Aitkulov Arman , Wolf Alexey , Dostovalov Alexander , Amantayeva Aida , Kurbanova Aliya , Inglezakis Vassilis J. , Tosi Daniele
Published: March 2021

Thermal ablation (TA) is known as an alternative therapy to surgery to treat tumors. However, TA-based therapy requires advanced approaches in order to prevent causing damage to healthy tissue around the tumor and selectively target the desired area. Nanoparticles are considered as a promising tool in biomedicine to fulfill these requirements. This study was carried out in order to analyze the effect of iron oxide nanoparticles on the temperature increment during radiofrequency ablation therapy of porcine liver. In addition, this research aimed to experimentally evaluate the impact of two solvents such as agarose and chitosan on the temperature change, when magnetic nanoparticles were dispersed in them. The iron oxide nanoparticles were synthesized by the solvothermal method demonstrating the magnetic properties by acting to the external magnetic field. To increase the local heat superparamagnetic nanoparticles (iron oxide magnetic nanoparticle (IONPs)) of the average size of 20 nm in size and the concentrations from 1 to 10 mg/mL of MNPs with a step size of 1 mg/mL were tested in 10 replicates for each concentration and solvent. Moreover, the temperature changes for dry liver, and 0 mg/mL concentration was checked for calibration and reference purposes. As a sensing system, advanced 16-FBG optical fiber sensors connected to an interrogator were employed allowing the temperature change to be monitored accurately in real time. A maximum temperature of about 142 °C was recorded by a 5 mg/mL concentration of iron oxide nanoparticles dispersed in the agarose solvent.
Numerical Optimization of a Microchannel Geometry for Nanofluid Flow and Heat Dissipation Assessment
Authors: Gonçalves Inês M. , Rocha César , Souza Reinaldo R. , Coutinho Gonçalo , Pereira Jose E. , Moita Ana S. , Moreira António L. N. , Lima Rui , Miranda João M.
Published: March 2021

In this study, a numerical approach was carried out to analyze the effects of different geometries of microchannel heat sinks on the forced convective heat transfer in single-phase flow. The simulations were performed using the commercially available software COMSOLMultiphysics 5.6® (Burlington, MA, USA) and its results were compared with those obtained from experimental tests performed in microchannel heat sinks of polydimethylsiloxane (PDMS). Distilled water was used as the working fluid under the laminar fluid flow regime, with a maximum Reynolds number of 293. Three sets of geometries were investigated: rectangular, triangular and circular. The different configurations were characterized based on the flow orientation, type of collector and number of parallel channels. The main results show that the rectangular shaped collector was the one that led to a greater uniformity in the distribution of the heat transfer in the microchannels. Similar results were also obtained for the circular shape. For the triangular geometry, however, a disturbance in the jet impingement was observed, leading to the least uniformity. The increase in the number of channels also enhanced the uniformity of the flow distribution and, consequently, improved the heat transfer performance, which must be considered to optimize new microchannel heat sink designs. The achieved optimized design for a heat sink, with microchannels for nanofluid flow and a higher heat dissipation rate, comprised a rectangular collector with eight microchannels and vertical placement of the inlet and outlet.
The Alterations in CD14 Expression in Periodontitis: A Systematic Review
Authors: Hirsch Vivian , Blufstein Alice , Behm Christian , Andrukhov Oleh
Published: March 2021

Objective: Cluster of differentiation (CD14) is an important protein involved in activating toll-like receptors by bacterial components. It exists as either a transmembrane or soluble protein, called mCD14 or sCD14, respectively. Several studies show that CD14 regulates the inflammatory response to periodontal pathogens, and its expression is altered in periodontitis, an inflammatory disease of tooth-supporting tissues. It is the intent of this review to investigate the levels of expression of mCD14 and sCD14 in peripheral blood monocytes, saliva, gingival crevicular fluid, and gingival tissue biopsies in periodontitis patients. Methods: PubMed, Scopus, Ovid/Medline, Embase, and the Cochrane Library were consulted for the online literature search. To ensure methodical quality, titles and abstracts were reviewed in accordance to the PRISMA guidelines. Data extraction and evaluation of the full texts were executed in agreement with the GRADE approach. Results: This systematic review shows that mCD14 levels are decreased in peripheral blood monocytes of periodontitis patients in comparison to healthy patients, while sCD14 levels in sera, gingival crevicular fluid (GCF), and biopsies of periodontitis patients have a tendency to be increased in comparison to healthy controls. The evaluation of CD14 in gingival biopsies and periodontal tissues elucidated the fact that interpretation of the data obtained with qPCR, ELISA, and flow cytometry is questionable.
Characteristics of Historical Precipitation in High Mountain Asia Based on a 15-Year High Resolution Dynamical Downscaling
Authors: Riley Collin , Rupper Summer , Steenburgh James W. , Strong Courtenay , Kochanski Adam K. , Wolvin Savanna
Published: March 2021

The mountains of High Mountain Asia serve as an important source of water for roughly one billion people living downstream. This research uses 15 years of dynamically downscaled precipitation produced by the Weather Research and Forecasting (WRF) model to delineate contrasts in precipitation characteristics and events between regions dominated by the Indian Summer Monsoon (ISM) versus westerly disturbances during the cool season (December to March). Cluster analysis reveals a more complex spatial pattern than indicated by some previous studies and illustrates the increasing importance of westerly disturbances at higher elevations. Although prior research suggests that a small number of westerly disturbances dominate precipitation in the western Himalaya and Karakoram, the WRF-downscaled precipitation is less dominated by infrequent large events. Integrated vapor transport (IVT) and precipitation are tightly coupled in both regions during the cool season, with precipitation maximizing for IVT from the south-southwest over the Karakoram and southeast-southwest over the western Himalaya. During the ISM, Karakoram precipitation is not strongly related to IVT direction, whereas over the western Himalaya, primary and secondary precipitation maxima occur for flow from the west-southwest and northwest, respectively. These differences in the drivers and timing of precipitation have implications for hydrology, glacier mass balance, snow accumulation, and their sensitivity to climate variability and change.
High-Resolution Numerical Modelling of Near-Surface Atmospheric Fields in the Complex Terrain of James Ross Island, Antarctic Peninsula
Authors: Matějka Michael , Láska Kamil , Jeklová Klára , Hošek Jiří
Published: March 2021

The Antarctic Peninsula belongs to the regions of the Earth that have seen the highest increase in air temperature in the past few decades. The warming is reflected in degradation of the cryospheric system. The impact of climate variability and interactions between the atmosphere and the cryosphere can be studied using numerical atmospheric models. In this study, the standard version of the Weather Research and Forecasting (WRF) model was validated on James Ross Island in the northern part of the Antarctic Peninsula. The aim of this study was to verify the WRF model output at 700 m horizontal resolution using air temperature, wind speed and wind direction observations from automatic weather stations on the Ulu Peninsula, the northernmost part of James Ross Island. Validation was carried out for two contrasting periods (summer and winter) in 2019/2020 to assess possible seasonal effects on model accuracy. Simulated air temperatures were in very good agreement with measurements (mean bias −1.7 °C to 1.4 °C). The exception was a strong air temperature inversion during two of the winter days when a significant positive bias occurred at the coastal and lower-altitude locations on the Ulu Peninsula. Further analysis of the WRF estimates showed a good skill in simulating near-surface wind speed with higher correlation coefficients in winter (0.81–0.93) than in summer (0.41–0.59). However, bias and RMSE for wind speed tended to be better in summer. The performance of three WRF boundary layer schemes (MYJ, MYNN, QNSE) was further evaluated. The QNSE scheme was generally more accurate than MYNN and MYJ, but the differences were quite small and varied with time and place. The MYNN and QNSE schemes tended to achieve better wind speed simulation quality than the MYJ scheme. The model successfully captured wind direction, showing only slight differences to the observed values. It was shown that at lower altitudes the performance of the model can vary greatly with time. The model results were more accurate during high wind speed southwestern flow, while the accuracy decreased under weak synoptic-scale forcing, accompanied by an occurrence of mesoscale atmospheric processes.
Butyrate Alleviates Cytokine-Induced Barrier Dysfunction by Modifying Claudin-2 Levels
Authors: Huang Xinyi , Oshima Tadayuki , Tomita Toshihiko , Fukui Hirokazu , Miwa Hiroto
Published: March 2021

Gastrointestinal (GI) disorders such as celiac disease and inflammatory bowel disease are attributed to intestinal barrier disruption. Imbalance of cytokines has been reported in the intestinal epithelium of patients with GI disorders. Short-chain fatty acids (SCFAs), derived from the fermentation of dietary fiber in the intestine, have been reported to benefit the intestinal barrier. Accordingly, we evaluated the effect of specific SCFAs on intestinal barrier function under cytokine-stimulated conditions. Caco-2 cells were cultured on insert membranes to generate monolayers, which then were used to investigate the effects of SCFAs. Tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), or interleukin-13 (IL-13) was added to the basolateral side of the membrane while SCFAs were added to the apical side. After a 24 h stimulation, transepithelial electrical resistance (TEER) was measured, and the protein levels of claudin-1, claudin-2, claudin-3, claudin-4, occludin, and zonula occludens-1 (ZO-1) were evaluated by Western blot. Butyrate, but not acetate, propionate, or succinate, ameliorated the TNF-α/IFN-γ-induced decrease in TEER. TNF-α/IFN-γ stimulation significantly increased the protein level of claudin-2 and decreased the level of claudin-3. Butyrate significantly attenuated the upregulation of claudin-2 induced by TNF-α/IFN-γ. Butyrate blocked the decrease in TEER and the upregulation of claudin-2 induced by IL-13 without changing the level of other tight junction proteins. Our results suggested that butyrate is the main component of SCFAs to alleviate barrier dysfunction and that claudin-2 is the major target of this SCFA.
3-Hydroxybutyrate as a Metabolite and a Signal Molecule Regulating Processes of Living Organisms
Authors: Mierziak Justyna , Burgberger Marta , Wojtasik Wioleta
Published: March 2021

3-hydroxybutyrate (3-HB) as a very important metabolite occurs in animals, bacteria and plants. It is well known that in animals, 3-HB is formed as a product of the normal metabolism of fatty acid oxidation and can therefore be used as an energy source in the absence of sufficient blood glucose. In microorganisms, 3-HB mainly serves as a substrate for the synthesis of polyhydroxybutyrate, which is a reserve material. Recent studies show that in plants, 3-HB acts as a regulatory molecule that most likely influences the expression of genes involved in DNA methylation, thereby altering DNA methylation levels. Additionally, in animals, 3-HB is not only an intermediate metabolite, but also an important regulatory molecule that can influence gene expression, lipid metabolism, neuronal function, and overall metabolic rate. Some of these effects are the direct effects of 3-HB itself, while others are indirect effects, regulated by the metabolites into which 3-HB is converted. One of the most important regulatory functions of 3-HB is the inhibition of the activity of histone deacetylases and thus the epigenetic regulation of many genes. Due to the number of functions of this compound, it also shows promising therapeutic properties.
Getting to Know Endometriosis-Related Infertility Better: A Review on How Endometriosis Affects Oocyte Quality and Embryo Development
Authors: Simopoulou Mara , Rapani Anna , Grigoriadis Sokratis , Pantou Agni , Tsioulou Petroula , Maziotis Evangelos , Tzanakaki Despina , Triantafyllidou Olga , Kalampokas Theodoros , Siristatidis Charalampos , Bakas Panagiotis , Vlahos Nikolaos
Published: March 2021

Endometriosis-related infertility describes a case of deteriorated fecundity when endometriosis is diagnosed. Numerous mechanisms have been proposed in an effort to delineate the multifaceted pathophysiology that induces impairment of reproductive dynamics in patients with endometriosis. In this critical analysis, authors present the plethora of molecular events that are entailed and elaborate on how they potentially impair the oocyte’s and embryo’s competence in patients with endometriosis. Reactive oxygen species, dysregulation of the immune system and cellular architectural disruption constitute the crucial mechanisms that detrimentally affect oocyte and embryo developmental potential. The molecular level impairment of the reproductive tissue is discussed, since differentiation, proliferation and apoptosis constitute focal regulatory cellular functions that appear severely compromised in cases of endometriosis. Mapping the precise molecular mechanisms entailed in endometriosis-related infertility may help delineate the complex nature of the disorder and bring us a step closer to a more personalized approach in understanding, diagnosing and managing endometriosis-related infertility.
The Effect of Maternal Immune Activation on Social Play-Induced Ultrasonic Vocalization in Rats
Authors: Gzielo Kinga , Potasiewicz Agnieszka , Litwa Ewa , Piotrowska Diana , Popik Piotr , Nikiforuk Agnieszka
Published: March 2021

Prenatal maternal infection is associated with an increased risk of various neurodevelopmental disorders, including autism spectrum disorders (ASD). Maternal immune activation (MIA) can be experimentally induced by prenatal administration of polyinosinic:polycytidylic acid (poly I:C), a synthetic viral-like double-stranded RNA. Although this MIA model is adopted in many studies, social and communicative deficits, included in the first diagnostic criterion of ASD, are poorly described in the offspring of poly(I:C)-exposed dams. This study aimed to characterize the impact of prenatal poly(I:C) exposure on socio-communicative behaviors in adolescent rats. For this purpose, social play behavior was assessed in both males and females. We also analyzed quantitative and structural changes in ultrasonic vocalizations (USVs) emitted by rats during the play test. Deficits of social play behaviors were evident only in male rats. Males also emitted a significantly decreased number of USVs during social encounters. Prenatal poly(I:C) exposure also affected acoustic call parameters, as reflected by the increased peak frequencies. Additionally, repetitive behaviors were demonstrated in autistic-like animals regardless of sex. This study demonstrates that prenatal poly(I:C) exposure impairs socio-communicative functioning in adolescent rats. USVs may be a useful tool for identifying early autistic-like abnormalities.
Limitations of Flow Diverters in Posterior Communicating Artery Aneurysms
Authors: ten Brinck Michelle F. M. , Rigante Luigi , Shimanskaya Viktoria E. , Bartels Ronald H. M. A. , Meijer Frederick J. A. , Wakhloo Ajay K. , de Vries Joost , Boogaarts Hieronymus D.
Published: March 2021

Background: Treatment of intracranial aneurysms with flow diverters (FDs) showed promising results. However, a subset of patients treated for posterior communicating artery (PComA) aneurysms has variable occlusion rates. Especially the fetal type-associated PComA aneurysms seemed to respond differently to treatment. We analyze our series of fetal type PComA aneurysms treated with a FD. The literature on this subject is reviewed. Methods: Data from patients treated with FD for all PComA aneurysms at the RadboudUMC Nijmegen were retrospectively analysed. Primary end-point was complete aneurysm occlusion at six months. Secondary end-points were clinical outcome, treatment safety, and results of secondary treatment after non-closure. The results for the fetal PComA aneurysms were compared to the literature. Results: Nineteen consecutive patients harboring 21 PComA aneurysms were treated. Three aneurysms had ipsilateral fetal type PCA (14.3%). Overall, none of the fetal type PcomA aneurysm showed complete occlusion versus 77.8% of the others (p = 0.03). Mortality and permanent morbidity rates were respectively 5.3% and 0%. Conclusions: FD treatment for PComA aneurysm with fetal type circulation seemed to be less effective compared to other types of PComA aneurysms. Flow characteristics at the PComA bifurcation are thought to be causative Alternative strategies should be considered as first line treatment.
Aspirin and Statin Use and the Risk of Gallbladder Cancer
Authors: Prasai Kritika , Tella Sri Harsha , Yadav Siddhartha , Kommalapati Anuhya , Mara Kristin , Mady Mohamed , Hassan Mohamed A. , Wongjarupong Nicha , Rodriguez-Payan Natalia , Borad Mitesh , Patel Tushar , Roberts Lewis R. , Mahipal Amit
Published: March 2021

Aspirin and statin drugs have been associated with reduced risk of several gastrointestinal cancers, but their association with gallbladder cancer (GBC) has not been well established. We evaluated the association of aspirin and statins with the risk of GBC. Patients with GBC managed at Mayo Clinic between 2000 and 2019 were matched 1:2 with a general patient pool by age and sex. Univariable and multivariable logistic regression models were used to assess associations between GBC and aspirin or statin use. The analysis included 795 cases and 1590 controls, with a median age of 67 years. Aspirin or statin use alone or in combination was higher in controls (p < 0.001). Univariate analysis showed that the use of aspirin [odds ratio (OR): 0.11; 95%CI: 0.08–0.15] or statins (OR: 0.29; 95%CI: 0.20–0.40) and their combined use (OR: 0.18; 95%CI: 0.13–0.24) was associated with lower risk of GBC. Multivariable analysis revealed that aspirin (OR: 0.12; 95%CI: 0.09–0.16) and combined statins and aspirin (OR: 0.46; 95%CI: 0.31–0.67) were associated with lower risk of GBC. Aspirin alone or in combination with statins is associated with a strongly reduced risk of GBC. Further prospective studies are needed to confirm these results and to elucidate their mechanisms.
Selective Catalytic Oxidation of Toluene to Benzaldehyde: Effect of Aging Time and Calcination Temperature Using CuxZnyO Mixed Metal Oxide Nanoparticles
Authors: Alharbi Khadijah H. , Alsalme Ali , Aloumi Ahmed Bader A. , Siddiqui Mohammed Rafiq H.
Published: March 2021

Oxidation is an important organic transformation, and several catalysts have been reported for this conversion. In this study, we report the synthesis of mixed metal oxide CuxZnyO, which is prepared by a coprecipitation method by varying the molar ratio of Cu and Zn in the catalytic system. The prepared mixed metal oxide CuxZnyO was evaluated for catalytic performance for toluene oxidation. Various parameters of the catalytic evaluation were studied in order to ascertain the optimum condition for the best catalytic performance. The results indicate that aging time, calcination temperature, reaction temperature, and feed rate influence catalytic performance. It was found that the catalyst interfaces apparently enhanced catalytic activity for toluene oxidation. The XRD diffractograms reveal the crystalline nature of the mixed metal oxide formed and also confirm the coexistence of hexagonal and monoclinic crystalline phases. The catalyst prepared by aging for 4 h and calcined at 450 °C was found to be the best for the conversion of toluene to benzaldehyde while the reactor temperature was maintained at 250 °C with toluene fed into the reactor at 0.01 mL/min. The catalyst was active for about 13 h.
Polyoxometalate Dicationic Ionic Liquids as Catalyst for Extractive Coupled Catalytic Oxidative Desulfurization
Authors: Li Jingwen , Guo Yanwen , Tan Junjun , Hu Bing
Published: March 2021

Wettability is an important factor affecting the performance of catalytic oxidative desulfurization. In order to develop an efficient catalyst for the extractive coupled catalytic oxidative desulfurization (ECODS) of fuel oil by H2O2 and acetonitrile, a novel family of imidazole-based polyoxometalate dicationic ionic liquids (POM-DILs) [Cn(MIM)2]PW12O40 (n = 2, 4, 6) was synthesized by modifying phosphotungstic acid (H3PW12O40) with double imidazole ionic liquid. These kinds of catalysts have good dispersity in oil phase and H2O2, which is conducive to the deep desulfurization of fuel oil. The catalytic performance of the catalysts was studied under different conditions by removing aromatic sulfur compound dibenzothiophene (DBT) from model oil. Results showed that [C2(MIM)2]PW12O40 had excellent desulfurization efficiency, and more than 98% of DBT was removed under optimum conditions. In addition, it also exhibited good recyclability, and activity with no significant decline after seven reaction cycles. Meanwhile, dibenzothiophene sulfone (DBTO2), the only oxidation product of DBT, was confirmed by Gas Chromatography-Mass Spectrometry (GC-MS), and a possible mechanism of the ECODS process was proposed.
Oxidative Stress and the Intersection of Oncogenic Signaling and Metabolism in Squamous Cell Carcinomas
Authors: Choe Joshua H. , Mazambani Simbarashe , Kim Tae Hoon , Kim Jung-whan
Published: March 2021

Squamous cell carcinomas (SCCs) arise from both stratified squamous and non-squamous epithelium of diverse anatomical sites and collectively represent one of the most frequent solid tumors, accounting for more than one million cancer deaths annually. Despite this prevalence, SCC patients have not fully benefited from recent advances in molecularly targeted therapy or immunotherapy. Rather, decades old platinum-based or radiation regimens retaining limited specificity to the unique characteristics of SCC remain first-line treatment options. Historically, a lack of a consolidated perspective on genetic aberrations driving oncogenic transformation and other such factors essential for SCC pathogenesis and intrinsic confounding cellular heterogeneity in SCC have contributed to a critical dearth in effective and specific therapies. However, emerging evidence characterizing the distinct genomic, epigenetic, and metabolic landscapes of SCC may be elucidating unifying features in a seemingly heterogeneous disease. In this review, by describing distinct metabolic alterations and genetic drivers of SCC revealed by recent studies, we aim to establish a conceptual framework for a previously unappreciated network of oncogenic signaling, redox perturbation, and metabolic reprogramming that may reveal targetable vulnerabilities at their intersection.
Photocontrollable Resistivity Change in Nanoparticle-Doped Liquid Crystal Alignment Layer: Voltage Holding and Discharging Properties of Fringe-Field Switching Liquid Crystal Modes
Authors: Ko Jeong-Hoon , Choi Jun-Chan , Lee Dong-Jin , Lee Jae-Won , Kim Hak-Rin
Published: March 2021

In liquid crystal (LC) displays, deriving an optimum resistance level of an LC alignment polyimide (PI) layer is important because of the trade-off between the voltage holding and surface-discharging properties. In particular, to apply a power-saving low-frequency operation scheme to fringe-field switching (FFS) LC modes with negative dielectric LC (n-LC), delicate material engineering is required to avoid surface-charge-dependent image flickering and sticking problems, which severely degrade with lowering operation frequency. Therefore, this paper proposes a photocontrolled variable-resistivity PI layer in order to systematically investigate the voltage holding and discharging properties of the FFS n-LC modes, according to the PI resistivity (ρ) levels. By doping fullerene into the high-ρ PI as the photoexcited charge-generating nanoparticles, the ρ levels of the PI were continuously controllable with a wide tunable range (0.95 × 1015 Ω∙cm to 5.36 × 1013 Ω∙cm) through Ar laser irradiation under the same LC and LC alignment conditions. The frequency-dependent voltage holding and discharge behaviors were analyzed with photocontrolled ρ variation. Thus, the proposed experimental scheme is a feasible approach in PI engineering for a power-saving low-frequency FFS n-LC mode without the image flickering and image sticking issues.
Seroprevalence of Anti-SARS-CoV-2 IgG and IgM among Adults over 65 Years Old in the South of Italy
Authors: Polvere Immacolata , Parrella Alfredina , Casamassa Giovanna , D’Andrea Silvia , Tizzano Annamaria , Cardinale Gaetano , Voccola Serena , Porcaro Piercarmine , Stilo Romania , Vito Pasquale , Zotti Tiziana
Published: March 2021

SARS-CoV-2 is a zoonotic betacoronavirus associated with worldwide transmission of COVID-19 disease. By the beginning of March, WHO reported about 113,820,000 confirmed cases including more than 2,527,000 deaths all over the world. However, the true extent of virus circulation or its real infection/fatality ratio is not well-estimated due to the huge portion of asymptomatic infections. In this observational study, we have estimated the prevalence of specific immunoglobulin M and G directed towards SARS-CoV-2 antigen in a cohort of 1383 adult volunteers aged over 65 years old, living in the district of Benevento, in the South of Italy. Serological screening was carried out on capillary blood in September 2020, seven months after pandemic outbreak in Italy, to evaluate virus circulation and antibody response among elderly adults, in which severe symptoms due to viral infection are more common. The overall seroprevalence of anti-SARS-CoV-2 antibodies was 4.70% (CI 3.70%–5.95%) with no statistically significant differences between sexes. Among these, 69.69% (CI 55.61%–77.80%) tested positive to IgM, 23.08% (CI 14.51%–34.64%) to IgG and 9.23% (CI 4.30%–18.71%) was positive for both. All patients that were positive to IgM underwent molecular testing through RT-qPCR on oral-rhino pharyngeal swabs and only one specimen was positive for SARS-CoV-2 RNA detection. Instead, the presence of IgG from screened volunteers was confirmed by re-testing serum samples using both an ELISA assay validated for in vitro diagnostic use (IVD) and a recently published synthetic peptide-based ELISA assay. In conclusion, our report suggests that (1) early restrictions were successful in limiting COVID-19 diffusion in the district of Benevento; (2) rapid serological analysis is an ideal testing for both determining real seroprevalence and massive screening, whereas detection of viral RNA remains a gold standard for identification of infected patients; (3) even among people without COVID-19 related symptoms, the antibody response against SARS-CoV-2 antigens has individual features.
Social Cognition in Multiple Sclerosis: A 3-Year Follow-Up MRI and Behavioral Study
Authors: Ziccardi Stefano , Pitteri Marco , Genova Helen M. , Calabrese Massimiliano
Published: March 2021

Social cognition (SC) has become a topic of widespread interest in the last decade. SC deficits were described in multiple sclerosis (MS) patients, in association with amygdala lesions, even in those without formal cognitive impairment. In this 3-year follow-up study, we aimed at longitudinally investigating the evolution of SC deficits and amygdala damage in a group of cognitive-normal MS patients, and the association between SC and psychological well-being. After 3 years (T3) from the baseline examination (T0), 26 relapsing-remitting MS patients (RRMS) were retested with a neuropsychological battery and SC tasks (theory of mind, facial emotion recognition, empathy). A SC composite score (SCcomp) was calculated for each patient. Emotional state, fatigue, and quality of life (QoL) were also evaluated. RRMS patients at T3 underwent a 3T-MRI as performed at T0, from which were calculated both volume and cortical lesion volume (CLV) of the amygdalae. Compared to T0, at T3 all RRMS patients were still cognitive-normal and remained stable in their global SC impaired performance. At T0, SCcomp correlated with amygdala CLV (p = 0.002) while, at T3, was more associated with amygdala volume (p = 0.035) rather than amygdala CLV (p = 0.043). SCcomp change T3-T0 correlated with global emotional state (p = 0.043), depression (p = 0.046), anxiety (p = 0.034), fatigue (p = 0.025), and QoL-social functioning (p = 0.033). We showed the longitudinal stability of SC deficits in cognitive-normal RRMS patients, mirroring the amygdala structural damage and the psychological well-being. These results highlight that SC exerts a key role in MS.