bluuebunny/crossref_metadata_2025 huggingface dataset:
Site-specific genome editing for hematopoetic stem cells transplantation-based gene therapy approaches
Authors: Lepik K. V , Popova M. O , Shakirova A. I , Sergeev V. S , Potter A. Y , Barkhatov I. M , Fehse B. , Afanasyev B. V
Published: October 2024

Allogeneic hematopoietic stem cell transplantation (HSCT) remains the sole universal curative approach for a number of hereditary diseases, such as severe combined immunodeficiency (SCID), severe non-SCID primary immunodeficiencies (non-SCID PID), hematologic diseases and part of lysosomal storage disorders. Unfortunately, to date, HSCT remains a high-risk procedure, especially in cases of poor performance status of the patient and lack of HLA-matched related donors. In those cases the correction of the patients autologous HSCs with gene therapy could be a promising alternative. Current paradigm of HSCT-based gene therapy approaches is based on the utilization of viral vectors, which may lead to the severe complications due to insertion mutagenesis. Throughout the last several years, new technologies of site-specific genome editing with endonucleases such as ZFNs, TALENs, and CRISPR/Cas9 were introduced. These enzymes may induce a DNA doublestranded break, homology-directed repair and insertion of functional copy of gene in precisely targeted locus. This review focuses on the advantages and disadvantages of the genome editing tools utilization that carries the great potential of changing the paradigm of gene therapy in the setting of HSCT.
Cell therapy of osteogenesis imperfecta
Authors: Sergeev V. S , Tichonenko T. I , Buklaev D. S , Baindurashvili A. G , Afanasiev B. V
Published: October 2024

Genetically determinated type I collagen structure anomaly causes the group of innate diseases known as osteogenesis imperfecta. Type I collagen is the basic component of the bone tissue organic matrix and is produced by osteoblasts. The replacement cell therapy can be a radical treatment option for the osteogenesis imperfecta if the high stable osteoblast chimerism level is reached. Analogously with the classical hematopoietic stem cell transplantation the replacement of hypothetic osteogenic stem cells is necessary for the stable osteoblasts engraftment. Stem cells with the potency to skeletal tissues differentiation were first described by A.J. Friedenstein in the bone marrow stroma. The evolution of the stromal stem cells vision leaded to the “mesenchymal stem cells”, “stem cells of skeletal tissues” and even “medical signal cells” concepts appearance. The transplantation of all listed cell types can lead to the increase of the clinical status in patients with osteogenesis imperfecta which is temporary yet. There was no success in the achievement of the stable osteoblasts engraftment during experimental and clinical studies at the moment. Such fact could be associated with the deficiency of fundamental knowledge about the source osteoblasts origin in vivo. At the same time taking into account extremely low range and poor efficiency of current severe osteogenesis imperfecta forms therapy approaches the cell therapy with ex vivo cultivated bone marrow stromal cells application is quite promising.
The effects of co-culture duration of cord blood cells with adipose tissue-derived stromal cells on hematopoietic precursors> amplification
Authors: Andreeva E. R , Andrianova I. V , Gornostaeva A. N , Bobyleva P. I , Balashova E. E , Buravkova L. B
Published: October 2024

Umbilical cord blood is considered as a valuable source of hematopoietic stem and progenitor cells (CB-HSPCs). The number of latter may be significantly enriched with ex vivo expansion. Thus, the optimization of culture conditions is essential for in vitro manipulations. Recently we have demonstrated that CB-HSPCs may be separated from unmanipulated CB nucleated cells through the adhesion to adipose tissue-derived MSCs. Further coculture was resulted in raizing of new polulation of floating CB-HSPCs significantly ehriched in primitive progentors The goal of this study was to optimize above mentioned protocol To determine the optimal conditions for adhesion and multiplication of CB-HSPCs, nucleated CB cells were co-cultured on adipose-tissue MSC layer for short (1-3 hours) and long-term (24-72 hours) duration Unattached cells were removed, adherent CB-HSPCs were further cultured for 72 hours, resulted in formation of floating population of CB-HSPCs. in each time point the number of attached CB-HSPCs, newly formed floating CB-HSPCs, CD34+ cells and CFUs among latter was examined. After 72 hours of nucleated CB cells co-culture, the number of adherent CD34+ cells peaked and was over than 70% of total CD34+ cells among nucleated CB cell samples Proposed experimental design has provided 4-fold enrichment of primitive CD34+ and 6-fold of CFUs number among newly formed HSPCs. BFU-Es comprised 80-90% of total CFUs regardless of time of nucleated CB cells coculture. Thus, 3 days of nucleated CB cells/adipose tissue-derived mesenchymal stromal cells co-culture provided peak of CD34+ cells' adhesion, amplification of latter resulted in rising of population maximally enriched both with undifferentiated and committed hematopoietic precursors
The role of LIF-STAT3 signaling in maintaining self-renewal and pluripotent state and in rat cells
Authors: Vaskova E. A , Sherstyuk V. V , Zakian S. M
Published: October 2024

Laboratory rat is one of the oldest and best studied objects of physiology and experimental medicine. However, rat pluripotent cells (embryonic and induced pluripotent stem cells) have been obtained relatively recently and remains poorly understood in terms of their transcriptome, proteome, epigenome as well as signaling cascades that maintain its self-renewal and pluripotency. In the study, the role of LIF-STAT3 cascade in maintaining self-renewal and pluripotency was investigated. It was shown that inhibition of the cascade led to cell cycle alteration, apoptotic and necrotic cell death. Additionally, RNA-seq data analysis was performed to identify transcripts level for LIF-STAT3 components. These results also suggest that LIF-STAT3 cascade plays an important role in self-renewal in rat pluripotent stem cells.
Development of tissue-engineered chitosan-polycaprolactone blends for vascular surgery
Authors: Zakharova I. S , Smirnova A. M , Zhiven' M. K , Saaya Sh. B , Shevchenko A. I , Zakian S. M , Ivanova L. N
Published: October 2024

Tissue engineering provides the opportunity to minimize some possible negative results of the synthetic vascular grafts in long-term follow-up. The choice of the optimal scaffold and cell source for seeding are key conditions to bring properties of vessel substitute to physiological. In some works it is shown that a chitosan-polycaprolactone blend is a suitable biodegradable material for tissue engineering. In this paper we suggest an efficient method to generate of tissue-engineered chitosan-polycaprolactone blends, cellularized by endothelial cells of human cardiac explants. The cells cultured on the blended membranes retain their functional properties: viability and proliferative properties; maintain specific endothelial antigens and synthesis of extracellular matrix. These results suggested that tissue-engineered chitosan-polycaprolactone blends seeded by endothelial cells of human cardiac explants may be potential to development of substitutes for small diameter blood vessels.
Cytokines and growth factors genes expression after subtotal liver resection in rats
Authors: Elchaninov A. V , Fatkhudinov T. Kh , Usman N. Y , Arutyunyan I. V , Makarov A. V , Kananykhina E. Y , Raimova E. Sh , Bolshakova G. B , Sukhikh G. T
Published: October 2024

Liver regeneration after 70% resection is one of the most studied models of tissue regeneration Small residual liver volume after massive hepatectomy or partial liver transplantation is a major cause of subsequent liver dysfunction known in oncology and transplantology as small-for-size syndrome The aim of this study was to evaluate the influence of 80% liver resection on cytokines and growth gens expression in rat Male Wistar rats were subjected to 80% and sacrificed at different times after surgery. Untreated animals served as controls Serum and liver samples were obtained to investigate liver function, cytokines and growth factors genes expression: il1b, il6, il10, tnfa, tweak, mmp9, fgf2, tgfb, hgf, vegf, sdfa, ang with quantitative RT-PCR. It was revealed two phases increased expression of genes that correspond to the first and second peak hepatocytes proliferation The first phase was characterized by increased expression mmp9, il6, il10, fgf2, tgfb. The second phase was characterized by increased expression of hgf, tnfa, tweak, il1b. Furthermore, we have found increased expression of the transcription factor SOX9 gene, and the gene encoding TWEAK, indicating a possible role for resident progenitor cells in the regenerating liver of rats after subtotal resection In our opinion the two phases of increased gene expression during liver regeneration in rats after subtotal resection are associated with two waves of hepatocyte proliferation, and the presence of growth factors reserves in the liver.
Problems of biomedical technologies legal regulation in Russia and abroad
Authors: Romanovsky G. B , Romanovskaya O. V
Published: October 2024

The work provides an overview of contemporary normative acts of the Russian Federation devoted to specific aspects of biomedicine: reproductive technologies, genetic engineering activity and genomic testing, treatment of embryos and fetal tissue. We analyze the draft federal law «On biomedical cell products», passed by the State Duma on 21 April 2015 in the first reading. The drawbacks of the project, presented the main points of view in terms of its legal regulation, formulated by a professional society of lawyers and experts in the field of cell technology. An overseas and international experience. In particular, the general provisions of the legislation of France, Germany, the Convention on Human Rights and Biomedicine, developed by the Council of Europe and adopted in Oviedo in 1997, the European Directive (2004/23 / EC) for the establishment of standards of quality and safety of organ donation, collection, testing, handling, storage and distribution of human tissues and cells We formulate a general conclusion on the need for the base of the Federal Law «On Biomedicine», which could only be resolved key issues that are at the peak of the scientific discussion of the professional community, affecting basic human rights All technological issues need to be resolved at the level of subordinate legislation
The dependence of proliferation rate of human dermal fibroblasts on growth medium composition and fetal bovine serum concentration
Authors: Fadeyev F. A , Lugovets D. V , Ulitko M. V , Leontyev S. L , Sazonov S. V
Published: October 2024

Application of dermal fibroblasts for therapy requires the use of large volume of cell material. The automatized cultivation provides stable conditions for GMP-grade cell production. To define the effect of serum concentration on proliferation rate, fibroblasts were cultured in growth media with different serum percentage. The proliferation rate was evaluated by the use of average period of cell population doubling. For choosing the optimal culture medium, different types of media together with their mixtures, produced by Gibco and PanEco, were explored. It was shown that exponential decrease of the period of cell population doubling occurs when the serum concentration rises from zero to 1 2%, and further increment of serum percentage is followed only by minor reduction of this value. The highest proliferation rate of dermal fibroblasts is obtained when they are cultivated in aMEM medium or in the mixtures of aMEM with F-12, Advanced DMEM with F-12 and Advanced DMEM with RPMI-1640 media (Gibco, USA).
Frequencies of the KITLG, BAK1 and SPRY4 alleles and genotypes associating with the development of testicular germ cell tumors, are increased in patients with testicular microlithiasis
Authors: Dantzev I. S , Ivkin E. V , Tryakin A. A , Bulanov A. A , Godlevski D. N , Latyshev O. Y , Rudenko V. V , Tyulyandin S. A , Volodko E. A , Okulov A. B , Loran O. B , Nemtsova M. V
Published: October 2024

Testicular microlithiasis is a random finding on ultrasound testicular examination. Testicular microlithiasis is of particular interest as an informative marker in men with an increased risk of testicular germ cell tumors. Genes KITLG, SPRY4 and BAK1 influence the development of the testes and spermatogenesis, their change results in a significant increase in the risk of testicular germ cell tumors. To determine the genetic factors that determine an increased risk of testicular germ cell tumors in patients with testicular microlithiasis, we investigated the frequency of alleles and genotypes of genes KITLG (rs995030, rs1508595), SPRY4 (rs4624820, rs6897876) and BAK1 (rs210138) in groups of fertile male, patients with testicular germ cell tumors and patients with testicular microlithiasis. For KITLG rs995030 we found significant differences in the frequency of the genotype GG in patients with testicular microlithiasis (p = 0.013) and in patients with testicular germ cell tumors (p = 0,0031) compared with the control. For KITLG rs1 508595 revealed significant differences in the frequency of G allele and GG genotype in patients with testicular microlithiasis (p = 0,002 for allele, p = 0,01 for genotype) and patients with testicular germ cell tumors (p = 0.0003 for allele; p = 0,014 for genotype). For BAK1 rs210138 we found significant differences in the frequency of G allele in patients with testicular microlithiasis, compared with the control group (p = 0,03). With a combination of high-risk genotypes study KITLG (rs995030, rs1508595) an BAK1 (rs210138) showed that the combination of these genotypes were significantly more common in patients with testicular germ cell tumors (p = 0,0001) and patients with testicular microlithiasis (p = 0,0053), on compared with the control. We have shown an increase in frequency of genotypes combination for testicular germ cell tumors at 4,6 times (OR: 4,669 [2,172-10,034]), and testicular microlithiasis - 3,3 times (OR: 3,355 [1,471-7,654]).
Plakophilin-2 gene knockout using CRISPR/Cas9 system
Authors: Khudiakov A. A , Kostina DA. A , Kostareva A. A , Malashicheva A. B
Published: October 2024

CRISPR/Cas9 technology opens up broad prospects for genome manipulations. Generation of gene knockout cell lines is one of the applications of this system. This approach allows to reveal previously unknown gene functions and to reproduce the effect of pathogenic mutations. The purpose of this study was to generate plakofilin-2 gene knockout system and assess its suitability for the study of the functional effect of this knockout.
Povyshenie effektivnosti zaseleniya biodegradiruemykh matriksov stromal'nymi i epitelial'nymi kletkami pri dinamicheskom kul'tivirovanii
Authors: Lyundup A. V , Demchenko A. G , Tenchurin T. H , Krasheninnikov M. E , Klabukov I. D , Shepelev A. D , Mamagulashvili V. G , Oganesyan R. V , Orehov A. S , Chvalun S. N , Dyuzheva T. G
Published: October 2024

Adhesion and proliferation of eucariotic cells are charac-terizated by high sensibility to the cultivation conditions, cell mediums, material surface properties, et al. The aim of this study was to improve seeding of stromal and epithelial cells on biodegradable matrices. We tested several polymers perspective for regenerative medicine: polycaprolacton, cellulose diacetate, PLGA, PLA/polycaprolacton. Electrospinning forming membranes had different porousity and fiber sizes. We developed a new method for 3D-matrix seeding with the use of rotation of scaffolds with cells. Based on optimal proliferation activity of the 3T3/NIH and MCF-7 cells we have chosen the scaffold compositions for multilayered cells seeding.
Molecular and cytogenetic features of primary myelofibrosis
Authors: Polushkina L. B , Martynkevich I. S , Shuvaev V. A , Fominykh M. S , Karyagina E. V , Savrilova A. M , Abdulkadyrov K. M
Published: October 2024

Primary myelofibrosis is a myeloproliferative neoplasm characterized by bone marrow fibrosis and the risk of leukemic transformation. Clonal hematopoiesis underlying this pathology is caused by transformation of hematopoietic stem cells by somatic mutations of the genome which may lead to both aberrant proliferation and differentiation. The variability of the clinical course and prognosis of primary myelofibrosis is largely determined by the spectrum of molecular and cytogenetic defects detected in tumor cells. This review describes the currently known somatic mutations defined in patients with primary myelofibrosis and possible ways of their pathogenic action are discussed. Recent data of the impact of molecular and cytogenetic abnormalities in clinical features and prognosis of the disease were analyzed.
C-kit and desmin-positive cells in expression in islets of pancreas during alloxan diabetes in rats
Authors: Kaligin M. S , Plushkina A. S , Titova A. A , Titova MA. A , Gumerova A. A , Kiassov A. P
Published: October 2024

Nowadays there are findings that C-kit-positive and desmin-positive stellate cells help to regenerate endocrine part of pancreas. But still it is unknown about their role and the way of differentiation of these cells during pancreas regeneration. That's why the aim of our work was to study the dynamic of these cells population during alloxan diabetes in rats. The work was made on 33 rats with experimental diabetes. Blood glucose, insulin and glucagon levels were analyzed. Also the expression of desmin (marker of stellate cells), a-SMA (marker of myofibroblast), C-kit (marker of endocrine stem cells), insulin and glucagon (marker of differentiated a- and р-cells of Langerhans islets) was studied. The expression of desmin was found after one day of experimental diabetes in islets cells of pancreas. Maximum of these cells was after the third day of the experiment. Also after one day of the experiment C-kit-positive cells, which expressed insulin and glucagon were found. We suppose that stellate cells are the main factor of microenvironment for differentiation of C-kit-positive progenitor cells into р-cells through the stage of glucagon-positive cells because stellate pancreas cells can produce different growth factors and components of intercellular matrics.
Use of stromal vascular fraction of adipose tissue for treatment of femur pseudoarthtosis: case report
Authors: Masgutov R. F , Salihov R. Z , Plaseichuk Yu. A , Salafutdinov I. I , Rizvanov A. A , Bogov A. A
Published: October 2024

The article presents a case report of successful treatment of pseudarthrosis of the femur with the use of autologous cells of the stromal vascular fraction (SVF) derived from adipose tissue. Autologous SVF cells mixed with fibrin glue were transplanted into bone defect by injection under the control of electro-optical converter. Two months after injection, the patient reported the disappearance of pain. After 4 months we observed the recovery of function of the knee, X-ray shows signs of the fracture healing through the formation of bone callus.
Delivery of cord blood cells modified with adenoviral vectors expressing GDNF into the area of spinal cord injury stimulates recovery of motor function and supports a population of glial cells
Authors: Mukhamedshina Y. O , Shaymardanova G. F , Salafutdinov I. I , Rizvanov A. A , Povysheva T. V , Masgutova G. A , Nigmetzyanova M. V , Chelyshev Yu. A
Published: October 2024

On the model of rat spinal cord dosed contusion at Th8 level studied the effect of delivery into the area of damage of glialcell-line-derived neurotrophic factor gene using adenovirus-transduced of human umbilical cord blood mononuclear cells on motor recovery and maintaining a population of glial cells. The results show that the proposed method of gene-cell therapy can effectively stimulate the regeneration of posttraumatic spinal cord injury, which is manifested in the form of improved indicators of recovery of motor function, increasing the number of reactive astrocytes and oligodendrocytes progenitors.
Long-term results of autologous peripheral blood hematopoietic stem cell transplantation in patients with peripheral arterial diseases
Authors: Plotnikov M. V , Maksimov A. V , Mavlikeev M. O , Gazizov I. M , Tamakova V. P , Cherepnev G. V , Shamsutdinova I. I , Gumerova A. A , Kiassov A. P
Published: October 2024

The aim of our work was to assess long-term results of autologous peripheral blood hematopoietic stem cell application in patients with peripheral arterial diseases. Peripheral blood hematopoietic stem cells mobilized by granulocyte colony-stimulating factor were transplanted intramuscularly to 30 patients with peripheral arterial diseases (IIb stage by Pokrovsky). Standart tredmill test, ankle-brachial index estimation and ankle-brachial index restoration time estimation after loading were performed on 0, 3, 6, 12 and 60 months after transplantation. Immunohistochemical study of injured gastrocnemius muscle biopsies taken before peripheral blood hematopoietic stem cells transplantation and 3 months after the procedure was performed. Peripheral blood hematopoietic stem cells transplantation increases capillary density (22,4%, p = 0,0005). Ankle- brachial index increased by 18,1% on month 6 after transplantation without a tendency to change on month 12. 60 months after transplantation initial to hematopoietic stem cells transplantation ankle-brachial index rates were marked. Painless walking distance was increasing at all times of observation progressively, on month 60 no walking distance limitation was marked by most patients. Ankle-brachial index restoring time shows positive trend of the functional state of limb during the first year after transplantation, 60 months after transplantation it showed no «walking reserve» limitation in most patients. 5-years survival was 79%, death causes were stroke, cardiac pathology (3 cases), lung cancer. So, peripheral blood hematopoietic stem cells transplantation allows eliminating peripheral arterial diseases symptoms and preserving limb in long-term period. Autologous transplantation of peripheral blood hematopoietic stem cells has no complications and is safe for therapy of patients with peripheral arterial diseases II stage.
Genomic and non-genomic effects of glucocorticoids
Authors: Todosenko N. M , Koroleva Yu. A , Khaziakhmatova O. G , Yurova K. A , Litvinova L. S
Published: October 2024

The overview describes the structure of a classic nuclear glucocorticoid receptor associated bound in inactive form to proteins chaperone complex. We analyzed genomic mechanisms regulated the gene expression, as well as a fastacting glucocorticoid effects of not genomic nature.
Comparative characterization of assessive methods of the cytotoxic properties of biological scaffolds
Authors: Kuevda E. V , Gubareva E. A , Sotnichenko A. S , Gumenyuk I. S , Gilevich I. V , Nakohov R. Z
Published: October 2024

Assessment of cell viability and metabolic activity on the scaffolds is an important part of the decellularized matrix characterization and its application possibilities to create tissue-engineered constructs. A variety of methods for assessing the matrix cytotoxic properties is not always characterized by methodological unity and has spread parameters using different protocols that require further investigation. On recellularized biological rat lung and diaphragm matrices the basic methods for determining the cytotoxic properties of biological scaffolds were studied. It is established that widely described in foreign literature colorimetric method for assessing the optical density of the treated and control cells and samples with the subsequent comparative analysis does not give a complete representation of cell viability on the scaffold. Calculation of cell viability based on mathematical formulas seems to be the most objective method of the cytotoxic properties of biological scaffolds evaluation. A promising method for assessing cell activity on biological scaffolds is to measure fluorescent activity of Alamar Blue reagent followed by quantification of cells proliferation. Colorimetric analyses of the absorption of the same agent without regard to the fluorescence afford terminated information and require the development of algorithms for the integrated assessment of cell viability on the scaffolds.
Analysis of regenerative medicine products market
Authors: Korsakov I. N , Nadelyaeva I. I , Eremin I. I , Pulin A. A , Kotenko K. V , Zorin V. L
Published: October 2024

In recent years, based on review of normative documentation of Russian Federation, United States of America, Europe and Asian countries, financial reports and press releases of commercial and non-commercial organizations acting in the field of biological technologies and regenerative medicine, clinical trials databases, information resources of state institutions regulating medicinal drugs and regenerative medicine products circulation, significant growth of regenerative medicine products at advanced clinical stages is brought out, as well as significant growth of cumulative investments for regenerative medicine industry and growth of investments for this line of business on the part of major pharmaceutical and biotechnological companies. Analysis of regenerative medicine industry was performed, main factors for its course of development were recognized, and recommendations were provided on development of new Russian regenerative medicine markets
Umbilical cord blood as a source for allogeneic stem cell transplantation in children (the experience of Moscow Stem Cell Bank)
Authors: Shamanskaya T V , Astrelina T A , Podkolzina Z A , Karpova E E , Lebedeva L L , Skorobogatova E E , Paina O B , Yakovleva M B , Shamanskaya T V , Astrelina Т А , Podkolzina E A , Karpova E E , Lebedeva L L , Skorobogatova E V , Paina O V , Yakovleva M V
Published: October 2024

The results of hematopoetic stem cell transplantation [HSCTJ of cord blood units collected by Stem Cell Bank of Moscow are described. From August 200Б till August 2009 1Б children with malignant hematological and non-malignant diseases have undergone umbilical cord blood transplantation ШСВТ). The median number of total nucleated cells and CD34+ cells infused was B,Ox107/kg [range 0,2-21,5) and 7,7x105/kg [range 0,5-40), accordingly. 9 (5B,25%) patients showed engraftment. The median time to neutrophil engraftment was 24 days [range 15-ЗБ). Acute GVHD grade l-ll was observed in 7[43,75%) patients, there were no cases of severe GVHD. Chronic GVHD was noted in KB,25%) patient. The transplant-related mortality at 100 days was 28,5%. The overall survival was 5B,2%, with a median time of observation of 11Б days. The results of our study showed that UCB is an alternative source of stem cells for HSCT in patients with malignant and non-malignant diseases.
GESTÃO DE SUSTENTABILIDADE EM PROCESSOS DE COMPRAS PÚBLICAS: UM ESTUDO EM INSTITUIÇÕES DE SAÚDE PÚBLICA BRASILEIRAS
Authors: Oliveira Fábio Batista de
Published: October 2024

A sustentabilidade é uma temática que vem se tornando cada vez mais importante em diferentes áreas sociais e econômicas na atualidade. Na gestão pública, em especial nas áreas da saúde já estão sendo implantadas práticas como o processo de licitação sustentável, com objetivo de diminuir danos ao meio ambiente e garantir melhor utilização de recursos pelo poder público.(Nonato, 2022.) Os apoios e os incentivos a prática do processo de licitação sustentável crescem a cada dia, tal iniciativa não deixa de seguir os ritos normais de uma licitação, assim como seu principal objetivo, o de adquirir seus itens através do menor preço. (Pedro Demarchi et al., 2022.) O conceito de compras sustentáveis traz incentivos a implementação de critérios de eficiência econômica e ambiental (de Oliveira & dos Santos, 2015). Este trabalho tem como objetivo propor uma revisão sistemática da literatura (RSL) com emprego das seguintes palavras-chave: licitação sustentável, práticas, sustentabilidade econômica, compras sustentáveis. Na metodologia foram selecionados trabalhos de acordo com o tema através da base scopus e web of sciences com proposta de análise para selecionar e identificar a relação com o tema e após avaliar e sintetizar as evidências que se apresentarem relevantes. Por meio deste trabalho, foi possível constatar que há expressiva participação em pesquisas de sustentabilidade nas compras públicas, porém ainda superficial em relação ao que precisa para tirar do papel o que dispõe a nova Lei de Licitações e contratos, a 14.133/2021. Como proposta para trabalhos futuros, identificamos a necessidade de continuidade dos estudos, em razão do fato de existirem, ainda, poucos estudos relacionados ao tema.
Проблемы чтения и понимания: от оцифрованного текста к гипертексту
Authors: Борисенко Наталья Анатольевна , Миронова Ксения Вадимовна
Published: October 2024

Поднимается проблема неоднородности цифрового чтения. По аналогии с континуумом реальности – виртуальности П. Милгрэма и Ф. Кишино предлагается рассматривать чтение с экрана не как единую монолитную систему, а как континуум, включающий множество модификаций. В теоретической части статьи представлена типология видов электронного текста и модификаций чтения. Анализируются как простые модификации (например, чтение оцифрованного текста), так и сложные, в том числе чтение гипертекста. Показано, что не только чтение интерактивного онлайн-текста, но и монокодового вербального в формате PDF существенно отличается от бумажного по ряду параметров. В качестве ключевого вопроса в проблематике цифрового чтения рассматривается проблема понимания. В практической части на выборке школьников 5–9-х классов представлены результаты эмпирического исследования половозрастных различий в уровне понимания монокодового оцифрованного текста. Выявлено, что при работе с бумажным текстом девочки успешнее (р ≤ 0,05), чем мальчики, выполняют задания на понимание, демонстрируя более внимательное отношение к фактуальной информации и лучше справляясь с определением концепта. Что касается чтения с экрана, значимых гендерных различий не обнаружено (р > 0,05).

In the article, the problem of heterogeneity of digital reading is raised. By analogy with Milgram and Kishino’s reality-virtuality continuum, it is proposed to consider screen reading not as a single monolithic system, but as a continuum that includes many modifications. The theoretical part of the article presents a typology of types of electronic text and modifications of reading. Both simple modifications (e.g., reading digitized text) and complex ones, including reading hypertext, are analyzed. It is shown that not only reading interactive online text, but also mono-code verbal text in PDF format differs significantly from paper reading in a number of parameters. Text comprehension is considered as a key issue in the problems of digital reading. In the practical part, using a sample of students in grades 5–9, the results of an empirical study of gender and age differences in the level of understanding of monocode digitized text are presented. It was revealed that when working with paper text, girls outperform boys in reading comprehension (p ≤ 0.05), demonstrating a more attentive attitude to factual information and better coping with tasks on conceptual information. Regarding screen reading, no significant gender differences were found (p > 0.05).
Analysis of Taurine Content in Microalgae Tetraselmis sp. Cultured at Different Salinities
Authors: Nurafiah Eva , Widiastuti Endang Linirin , Maharani Henni Wijayanti
Published: October 2024

Taurine is a non-essential sulfur-containing amino acid with the chemical formula C2H7NO3S. These compounds play an important role in maintaining the smooth running of various processes in the body. Tetraselmis sp. is one of the microalgae known to have a taurine biosynthetic pathway via the serine/sulfate pathway. The purpose of this study was to determine the effect of culture salinity on taurine content produced by microalgae Tetraselmis sp. which was dissolved using 96% ethanol. The samples obtained were then tested using UV-Vis spectrophotometry using standard synthetic taurine as a comparison. Based on the research results, the maximum absorption was obtained at a wavelength of 630 nm, and the regression equation y = 0.001x + 0.033 was obtained and had a correlation coefficient of r = 1. Based on the analysis results showed that all samples contained taurine. Samples at a salinity of 25 ppt contained an average of 2.82 ml/100g of taurine, while a culture salinity of 35 ppt contained an average of 4.03 ml/100g of taurine. From these data it is known that the highest taurine level was found at a culture salinity of 35 ppt, while the lowest taurine level was at a culture salinity of 25 ppt. This indicates that the higher the culture salinity, the higher the taurine content produced by the microalgae Tetraselmis sp. The phenomenon of increased taurine due to salinity stress is thought to be due to an increase in free amino acids in microalgae cells to produce metabolites that are used to defend themselves due to salinity stress. The function of taurine in this microalgae is thought to be an organic osmolyte in the cells of the Tetraselmis sp. microalgae.
V.V. Rozanov and the Tsvetaev Brothers
Authors: Aleksey Lomonosov
Published: October 2024

The article, based on the correspondence of Ivan Vladimirovich and Dmitry Vladimirovich Tsvetaev brothers with V. V. Rozanov, reveals the history of creative relationship between famous historians and the philosopher. The little-known history of V.V. Rozanov's relationship with historian D.V. Tsvetaev is presented. For the fi rst time the author examines the letters of V.V. Rozanov and I.V. Tsvetaev, previously barely known to the scientifi c community. Their content explains the position of the editors of the “Novoye Vremya” journal in the legal proceedings between Professor I.V. Tsvetaev and the Minister of Public Education A.N. Schwartz. The article entails the publication of V.V. Rozanov and I.V. Tsvetaev letters discovered to date. The comments for the fi rst time present variants of the editorial proof-reading given by V.V. Rozanov at the time of I.V. Tsvetaev letter fragments publication.
Pelatihan Pendamping Nagari dalam Penyusunan Instrumen Survei Kependudukan Berbasiskan Digital di Nagari Anduring Kecamatan 2x11 Kayu Tanam Kabupaten Padang Pariaman
Authors: Suasti Yurni , Ramadhan Risky , Ikhwan Ikhwan , Frananda Hendry
Published: October 2024

Ketersediaan data kependudukan diberbagai tingkatan administrasi pemerintahan menjadi faktor kunci keberhasilan pelaksanaan program-program pembangunan maupun penyelenggaraan pemerintahan. Kenagarian Anduring memiliki 7 Korong yang sangat luas dan tersebar hingga perbatasan Kecamatan Lubuk Alung. Tersebarnya Korong tersebut membuat permasalahan kependudukan menjadi hal utama di Nagari ini. Berdasarkan penelusuran Lapangan yang dilakukan, data kependudukan di wilayah studi masih bersifat umum dan belum mencerminkan amanat UU No 24 Tahun 2013 tentang administrasi kependudukan. Permasalahan mitra yaitu (1) Belum tersedianya database kependudukan yang sistematik, terstruktur dan terintegrasi digital (2) Belum terkumpulnya data agregat kependudukan sesuai UU No 24 Tahun 2013 (3) Kurangnya kemampuan survey Lapangan kependudukan oleh Kader KB di Nagari Anduring. Solusi untuk mengatasi permasalahan diatas yaitu (1) Workshop dan FGD Bersama Mitra terkait “Data Kependudukan Terdigitalisasi menuju era 4.0” (2) Pelatihan penyusunan instrument kependudukan dengan kerja sama mitra yaitu Disdukcapil Provinsi Sumatera Barat. (3) Pelatihan survey Lapangan kependudukan melalui aplikasi Open Data Collect yang komprehensif dan teritegrasi dengan sistem informasi. Metode yang digunakan dalam pengabdian masyarakat ini adalah penerapan model Participatory Rural Appraisal (PRA). PRA merupakan metode penelitian aksi yang dikembangkan untuk meningkatkan partisipasi masyarakat/Perangkat nagari/pendamping desa/kader KB dalam pembangunan. Dimulai dari workshop dan FGD kemudian dilanjutkan pelatihan penyusunan instrumen survei kependudukan menggunakan aplikasi ODK Collect yang open acces.
Sosialisasi Pencegahan Radikalisme Pada Anak Usia Dini di RA Darussaadah Palangka Raya
Authors: Afifah Neela , Muzakki Muzakki , Aghanaita Aghanaita , Hidayati Sri , Setyawati Setyawati , Khairunnisa Khirunnisa
Published: October 2024

Pengabdian masyarakat ini dilatarbelakangi oleh minimnya pengetahuan guru tentang radikalisme dan cara pencegahannya, terutama pada anak usia dini. Oleh karena itu, perlu adanya sosialisasi mengenai radikalisme pada anak usia dini dan cara pencegahannya. Tujuan dari sosialisasi ini adalah untuk memberikan pemahaman, pengetahuan kepada guru tentang pencegahan radikalisme pada anak usia dini. Adapun sasaran dari sosialisasi ini adalah guru-guru di RA Darussa’adah Palangka Raya. Kegiatan pengabdian masyarakat ini dilaksanakan pada tanggal 25 November 2023 pada pukul 09.00 WIB sampai Selesai. Pada pelaksanaan kegiatan sosialisasi tersebut menunjukan bahwa semua peserta sangat antusias dalam mendengarkan pemaparan materi yang disampaikan dan aktif bertanya serta berdiskusi tentang radikalisme pada anak usia dini. Adapun peserta yang mengikuti kegiatan pengabdian ini terdiri dari 10 orang guru, 13 orang mahasiswa dan 4 orang dosen. Adapun hasil dari pengabdian dosen dan mahasiswa tentang sosialisasi pencegahan radikalisme pada anak usia dini di RA Darussa’adah Palangka Raya ialah guru dan mahasiswa yang berhadir dapat memahami tentang akibat, dampak atau bahaya dari radikalisme, serta mengetahui cara pencegahannya. Sehingga guru akan melakukan upaya penegahan radikalisme pada anak dengan cara membatasi penggunaan media sosial yang berlebihan, mengontrol tontonan anak-anak, dan pergaulan anak-anak.
Pelatihan Komputasi Tools Data Analysis untuk Meningkatkan Kompetensi Guru di Kepulauan Seribu
Authors: Santi Vera Maya , Rafli Muhammad , Indiyah Fariani Hermin , Rahayu Widyanti , Suyono Suyono , Handayani Dian , Sumargo Bagus , Siregar Dania , Ladayya Faroh
Published: October 2024

Peningkatan kemampuan analisis data kuantitatif bagi guru-guru menjadi suatu kebutuhan penting dalam konteks penelitian. Tingkat pemahaman analisis data kuantitatif guru yang masih rendah menegaskan perlunya program peningkatan kompetensi guru melalui pelatihan. Tujuan dari penelitian ini adalah untuk memperkuat kompetensi guru dalam mengembangkan dan menerapkan penelitian, khususnya dalam materi analisis statistika kuantitatif dengan menggunakan fitur analisis data di excel. Pelatihan ini diselenggarakan untuk guru SMP dan SMA di Kepulauan Seribu, melalui pendekatan ceramah, demonstrasi, dan praktik, diikuti oleh 23 peserta, yang terdiri dari 14 guru SMK dan 9 guru SMP. Berdasarkan analisis pre-test dan post-test dengan uji-t berpasangan, hasil menunjukkan bahwa p-value pada pre-test dan post-test adalah kurang dari α (0,05). Hal ini mengindikasikan bahwa peserta pelatihan mendapatkan pengetahuan baru, meningkatkan motivasi, dan keterampilan yang mendukung profesinya sebagai guru setelah mengikuti pelatihan.
Pendampingan Optimalisasi Social Commerce Bagi UMKM di Kelurahan Wangurer Barat Kota Bitung
Authors: Pandowo Aditya , Evinita Lenny
Published: October 2024

Penggunaan teknologi informasi sebagai alat penunjang promosi menjadi semakin hegemonik semenjak Covid 19 melanda dunia. Perpindahan pola pembelian secara daring turut mendukung percepatan penggunaan social commerce. Sesuai dengan visi dan misi pemerintah Kota Bitung yang hendak mewujudkan Kota Bitung sebagai kota digital menuju Smart City, maka dipandang perlu untuk memberikan pembekalan dalam bentuk pendampingan bagi pelaku UMKM Kota Bitung khususnya yang berada di Kelurahan Wangurer Barat, Kecamatan Madidir. Adapun yang terlibat dalam kegiatan ini adalah pemilik warung kelontongan, kuliner, dan bengkel sederhana. Pelaksanan pembekalan dilakukan dengan menggunakan metode ceramah, tutorial, praktik mandiri, dan diskusi dengan total kegiatan selama 8 jam. Temuan dalam kegiatan pengabdian ini menunjukkan kecenderungan penggunaan media sosial dan marketplace lainnya belum dilakukan secara optimal. Dengan pelaksanaan program pengabdian pada masyarakat ini, diharapkan adanya peningkatan penghasilan dan memperluas jangkauan penjualan bagi pelaku UMKM dalam memasarkan produknya melalui program pemasaran melalui media sosial.
Peningkatan Citra Produk Sentra Olahan Herbal Surabaya Melalui Sosialisasi Kemasan Ramah Lingkungan
Authors: Khoirotunnisa Fani
Published: October 2024

Kesadaran masyarakat tentang kelestarian lingkungan dan konsumsi produk ramah lingkungan meningkat. Sentra olahan herbal di Surabaya, dengan potensi besar dalam menyediakan minuman herbal sehat, menghadapi tantangan populer di kalangan generasi muda karena kemasan yang kurang menarik dan strategi pemasaran tradisional. Isu utama adalah desain kemasan yang ketinggalan zaman dan kurangnya inovasi pemasaran. Pengabdian ini bertujuan untuk meningkatkan citra produk melalui sosialisasi dan pelatihan tentang desain kemasan ramah lingkungan serta strategi pemasaran modern. Hasilnya menunjukkan perbaikan kualitas kemasan dan perluasan distribusi produk UMKM, baik di toko oleh-oleh maupun platform online. Implikasi penelitian ini adalah peningkatan daya saing produk herbal dan kontribusi pada keberlanjutan lingkungan
Usaha Pengolahan Ikan Siap Saji dari Ikan Segar Sebagai Pengganti Ikan Asin
Authors: Rini Tjipto , Sajekti Tjipto
Published: October 2024

Dampak pandemi Covid-19 menyebabkan daya beli masyarakat menurun meskipun itu daya beli makanan. Penjual ikan segar dan pengolah ikan asin juga mengalami kerugian karena  hasil penangkapan ikan segar sangat berlimpah. Untuk mengatasi hal tersebut, para nelayan harus bisa mengubah cara penjualan. Tujuan kegiatan yaitu : melaksanakan penyuluhan kesehatan tentang bahaya formalin dan H2O2, melaksanakan pelatihan membuat ikan frozen dari ikan segar yang tahan sampai 7 bulan  serta pelatihan membuat laporan keuangan sederhana. Sebanyak 40 orang ibu-ibu nelayan ikut dalam kegiatan ini. Setelah 2 hari mengikuti kegiatan pengabdian masyarakat, kegiatan tersebut dievaluasi. Ternyata yang ikut kegiatan sampai selesai,  dan yang sudah bisa membuat ikan frozen serta bisa membuat laporan keuangan sederhana berjumlah 32 orang, berarti keberhasilan mencapai 80%. Seiring berjalannya waktu dan dengan pendampingan terus menerus oleh tenaga Puskesmas  (yang bertugas mengawasi produksi olahan mereka),  suatu saat nelayan di daerah Cilincing tersebut akan merubah cara pengolahan ikan hasil tangkapan mereka.
Evaluasi Capstone Project Desain Antarmuka Aplikasi untuk Guru MGMP TIK Surabaya
Authors: Ahmadiyah Adhatus Solichah , Sarno Riyanarto , Hidayati Shintami Chusnul , Anggraini Ratih Nur Esti , Sungkono Kelly Rosa , Munif Abdul
Published: October 2024

Peningkatan keterampilan desain aplikasi bagi guru MGMP TIK Surabaya penting untuk ditingkatkan guna mendukung kualitas pendidikan yang lebih baik. Kegiatan pengabdian kepada masyarakat ini dilakukan oleh tim dosen dan mahasiswa laboratorium manajemen cerdas informasi teknik informatika ITS dengan tujuan memberikan rekomendasi dan masukan terkait permasalahan desain antarmuka aplikasi yang dibuat oleh guru peserta pelatihan. Metode yang digunakan adalah evaluasi heuristik dan klasifikasi permasalahan menggunakan severity level. Hasil dari kegiatan ini menunjukkan bahwa guru menjadi tahu apa permasalahan yang ada di desain antarmuka yang dibuat dan mengetahui aksi perbaikan. Hal ini juga memperjelas penyampaian materi dengan permasalahan dan solusi nyata, sehingga dapat meningkatkan kualitas pembelajaran dan pengajaran
Pelatihan Pemasaran Digital padaYouth Centre Training Progragonist Generation for Society (SF-JPS) Timor Leste
Authors: Sakti Imanuel Madea , Belo Domingas Ximenes
Published: October 2024

Perkembangan teknologi digital yang pesat perlu diikuti dengan peningkatan kompetensi individu, salah satunya adalah pemasaran digital. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan kemampuan pemasaran digital melalui metode pelatihan. Peserta kegiatan adalah pengurus dan siswa dari Youth Centre Training Progragonist Generation for Society Timor Leste (Jerasaun Protagonista ba Sosiedade/SF-JPS) sebanyak 20 peserta dan dilaksanakan dengan metode pelatihan berupa pemaparan materi dan demonstrasi secara online melalui Zoom Meeting. Materi yang disampaikan dalam pelatihan mencakup penjelasan mengenai pemasaran digital dan diikuti dengan penjelasan dan praktik pembuatan desain grafis dengan aplikasi Canva serta pembuatan website dengan aplikasi Google Sites. Secara keseluruhan, hasil pelatihan menunjukkan bahwa peserta memperoleh pengetahuan dan keterampilan baru dalam hal kompetensi pemasaran digital. Dalam hal pelaksanaan kegiatan, terdapat beberapa keterbatasan seperti koneksi internet, perangkat komputer, dan durasi kegiatan yang belum maksimal.
Strategi Adaptasi Guru Muslim di Sekolah Katolik (Studi Kasus: Yayasan Prayoga Padang)
Authors: Fernanda Sari Maria , Nora Desri
Published: October 2024

Penelitian ini bertujuan untuk menganalisis bagaimana strategi adaptasi guru yang beragama muslim di sekolah yang mayoritas beragama katolik. Penelitian ini dilaksanakan di SMA Katolik Xaverius Padang dan SMA Don Bosco Padang. Penelitian ini dianalisis dengan menggunakan teori Strucktural Fungsional dengan skema AGIL yang dikemukan Talcott Parsons. Penelitian ini menggunakan pendekatan kualitatif dengan tipe penelitian studi kasus. Pemilihan informan dilakukan secara purposive sampling dengan jumlah informan 19 orang. Pengumpulan data dilakukan dengan cara observasi, studi dokumentasi dan wawancara mendalam. Keabsahan data dilakukan dengan teknik triangulasi data. Analisis data yang dilakukan adalah model Interaktif Miles dan Huberman. Hasil penelitian dari strategi adaptasi guru muslim di sekolah katolik adalah bagaimana guru muslim beradaptasi dengan rekan guru yang beragama lain dimana dalam beradaptasi dengan rekan guru yang berbeda keyakinan guru yang bearagama katolik terbuka terhadap guru yang beragama muslim meskipun guru beragama muslim merupakan minoritas di sekolah tetapi hal tersebut bukan menjadi masalah bagi guru yang beragama non muslim, justru para guru tersebut menjunjung toleransi yang tinggi. Kedua peduli terhadap sesama dimana seperti saat sholat guru muslim melakukan sholat di perpustakaan dan guru yang beragama non muslim pun menghargai dengan tidak ribut dan mengganggu guru yang sedang sholat, begitu juga sebaliknya saat guru yang beragama katolik melakukan pembiasaan atau doa angelus jam 12.00 WIB guru yang beragama muslim pun menghargai. Ketiga adalah keterlibatan dalam kegiatan di sekolah.