bluuebunny/crossref_metadata_2025 huggingface dataset:
Sasa quelpaertensis Leaf Extract Ameliorates Dyslipidemia, Insulin Resistance, and Hepatic Lipid Accumulation in High-Fructose-Diet-Fed Rats
Authors: Park Jeong Yong , Jang Mi Gyeong , Oh Jung Min , Ko Hee Chul , Hur Sung-Pyo , Kim Jae-Won , Baek Songyee , Kim Se-Jae
Published: December 2020

Background: Increased dietary fructose consumption is closely associated with lipid and glucose metabolic disorders. Sasa quelpaertensis Nakai possesses various health-promoting properties, but there has been no research on its protective effect against fructose-induced metabolic dysfunction. In this study, we investigated the effects of S. quelpaertensis leaf extract (SQE) on metabolic dysfunction in high-fructose-diet-fed rats. Methods: Animals were fed a 46% carbohydrate diet, a 60% high-fructose diet, or a 60% high-fructose diet with SQE (500 mg/kg of body weight (BW)/day) in drinking water for 16 weeks. Serum biochemical parameters were measured and the effects of SQE on hepatic histology, protein expression, and transcriptome profiles were investigated. Results: SQE improved dyslipidemia and insulin resistance induced in high-fructose-diet-fed rats. SQE ameliorated the lipid accumulation and inflammatory response in liver tissues by modulating the expressions of key proteins related to lipid metabolism and antioxidant response. SQE significantly enriched the genes related to the metabolic pathway, namely, the tumor necrosis factor (TNF) signaling pathway and the PI3K-Akt signaling pathway. Conclusions: SQE could effectively prevent dyslipidemia, insulin resistance, and hepatic lipid accumulation by regulation of metabolism-related gene expressions, suggesting its role as a functional ingredient to prevent lifestyle-related metabolic disorders.
Bactericides Based on Copper Nanoparticles Restrain Growth of Important Plant Pathogens
Authors: Varympopi Adamantia , Dimopoulou Anastasia , Theologidis Ioannis , Karamanidou Theodora , Kaldeli Kerou Alexandra , Vlachou Afroditi , Karfaridis Dimitrios , Papafotis Dimitris , Hatzinikolaou Dimitris G. , Tsouknidas Alexander , Skandalis Nicholas
Published: December 2020

Copper nanoparticles (CuNPs) can offer an alternative to conventional copper bactericides and possibly slow down the development of bacterial resistance. This will consequently lower the accumulation rate of copper to soil and water and lower the environmental and health burden imposed by copper application. Physical and chemical methods have been reported to synthesize CuNPs but their use as bactericides in plants has been understudied. In this study, two different CuNPs products have been developed, CuNP1 and CuNP2 in two respective concentrations (1500 ppm or 300 ppm). Both products were characterized using Dynamic Light Scattering, Transmission Electron Microscopy, Attenuated Total Reflection measurements, X-ray Photoelectron Spectroscopy, X-ray Diffraction and Scattering, and Laser Doppler Electrophoresis. They were evaluated for their antibacterial efficacy in vitro against the gram-negative species Agrobacterium tumefaciens, Dickeya dadantii, Erwinia amylovora, Pectobacterium carotovorum, Pseudomonas corrugata, Pseudomonas savastanoi pv. savastanoi, and Xanthomonas campestris pv. campestris. Evaluation was based on comparisons with two commercial bactericides: Kocide (copper hydroxide) and Nordox (copper oxide). CuNP1 inhibited the growth of five species, restrained the growth of P. corrugata, and had no effect in X. c. pv campestris. MICs were significantly lower than those of the commercial formulations. CuNP2 inhibited the growth of E. amylovora and restrained growth of P. s. pv. savastanoi. Again, its overall activity was higher compared to commercial formulations. An extensive in vitro evaluation of CuNPs that show higher potential compared to their conventional counterpart is reported for the first time and suggests that synthesis of stable CuNPs can lead to the development of low-cost sustainable commercial products.
Occurrence and Role of Selected RNA-Viruses as Potential Causative Agents of Watery Droppings in Pigeons
Authors: Łukaszuk Ewa , Stenzel Tomasz
Published: December 2020

The diseases with watery droppings (diarrhea and/or polyuria) can be considered some of the most severe health problems in domestic pigeons of various ages. Although they do not always lead to bird death, they can contribute to poor weight gains and hindered development of young pigeons and, potentially, to poor racing results in sports birds. The gastrointestinal tract disorders of pigeons may be of various etiology, but some of the causative agents are viral infections. This review article provides information collected from scientific reports on RNA-viruses belonging to the Astroviridae, Picornaviridae, and Coronaviridae families; the Avulavirinae subfamily; and the Rotavirus genus that might be implicated in such health problems. It presents a brief characterization, and possible interspecies transmission of these viruses. We believe that this review article will help clinical signs of infection, isolation methods, occurrence in pigeons and poultry, systemize and summarize knowledge on pigeon enteropathogenic viruses and raise awareness of the importance of disease control in pigeons.
Whole Genome Characterization and Evolutionary Analysis of G1P[8] Rotavirus A Strains during the Pre- and Post-Vaccine Periods in Mozambique (2012–2017)
Authors: Munlela Benilde , João Eva D. , Donato Celeste M. , Strydom Amy , Boene Simone S. , Chissaque Assucênio , Bauhofer Adilson F. L. , Langa Jerónimo , Cassocera Marta , Cossa-Moiane Idalécia , Chilaúle Jorfélia J. , O’Neill Hester G. , de Deus Nilsa
Published: December 2020

Mozambique introduced the Rotarix® vaccine (GSK Biologicals, Rixensart, Belgium) into the National Immunization Program in September 2015. Although G1P[8] was one of the most prevalent genotypes between 2012 and 2017 in Mozambique, no complete genomes had been sequenced to date. Here we report whole genome sequence analysis for 36 G1P[8] strains using an Illumina MiSeq platform. All strains exhibited a Wa-like genetic backbone (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). Phylogenetic analysis showed that most of the Mozambican strains clustered closely together in a conserved clade for the entire genome. No distinct clustering for pre- and post-vaccine strains were observed. These findings may suggest no selective pressure by the introduction of the Rotarix® vaccine in 2015. Two strains (HJM1646 and HGM0544) showed varied clustering for the entire genome, suggesting reassortment, whereas a further strain obtained from a rural area (MAN0033) clustered separately for all gene segments. Bayesian analysis for the VP7 and VP4 encoding gene segments supported the phylogenetic analysis and indicated a possible introduction from India around 2011.7 and 2013.0 for the main Mozambican clade. Continued monitoring of rotavirus strains in the post-vaccine period is required to fully understand the impact of vaccine introduction on the diversity and evolution of rotavirus strains.
Clinic- and Hospital-Based Home Care, Outpatient Parenteral Antimicrobial Therapy (OPAT) and the Evolving Clinical Responsibilities of the Pharmacist
Authors: Docherty Toni , Schneider Jennifer J. , Cooper Joyce
Published: December 2020

Clinic- and hospital-based home care describes models of care where services commonly associated with hospital inpatient care are provided at the patient’s home or in an outpatient or community-based clinic. Hospital in the Home (HITH), also termed Hospital at Home (HaH) in parts of Europe and America, is a common and important example of this type of care. Other examples include infusion centers, skilled nursing facilities (particularly in the USA), self-administration models (including home infusion services) and administration through outpatient or community clinics. Different models of HITH care are used internationally and these encompass a wide range of services. Medication administration, particularly outpatient parenteral antimicrobial therapy (OPAT), is an important element in many of these models of care. There is a key role for pharmacists since the provision of medication is integral in this model of patient care outside the hospital setting. Data on the growing importance of HITH and OPAT as well as the administration of medications suited to clinic- and hospital-based home care, including subcutaneous and intramuscular injectables, immunoglobulins and other blood fractions, cancer chemotherapy, total parenteral nutrition, biologicals/biosimilars, vasopressors and enzymes, using differing service models, are described. The pharmacist’s role is evolving from that involved primarily with dose preparation and supply of medications. Their clinical expertise in medication management ensures that they are an integral member and leader in these models of care. Their role ensures the safe and quality use of medicines, particularly across transitions of care, with the pharmacist taking on the roles of educator and consultant to patients and health professional colleagues. Activities such as antimicrobial stewardship and ongoing monitoring of patients and outcomes is fundamental to ensure quality patient outcomes in these settings.
Warping Effects in Strongly Perturbed Metrics
Authors: Frasca Marco , Liberati Riccardo Maria , Rossi Massimiliano
Published: December 2020

A technique devised some years ago permits us to develop a theory regarding a regime of strong perturbations. This translates into a gradient expansion that, at the leading order, can recover the Belinsky-Kalathnikov-Lifshitz solution for general relativity. We solve exactly the leading order Einstein equations in a spherical symmetric case, assuming a Schwarzschild metric under the effect of a time-dependent perturbation, and we show that the 4-velocity in such a case is multiplied by an exponential warp factor when the perturbation is properly applied. This factor is always greater than one. We will give a closed form solution of this factor for a simple case. Some numerical examples are also given.
Religious Call in Eastern Orthodox Spirituality: A Theo-Phenomenological Approach
Authors: Turcan Nicolae
Published: December 2020

Drawing a clear line between phenomenology and theology remains a challenging endeavor. This article has two parts: The first one argues that, from a methodological point of view, there is a need for a theo-phenomenology, a phenomenology which acknowledges religious faith as a given. The second part of the article tries to present the essence of religious call in Eastern Orthodox spirituality. Using ideas such as appeal and communion, divine grace, love, prayer, fidelity, apophatic intentionality, and a hyper-intelligible gaze before the Revelation, I will describe the phenomenon of religious call—God calling man and man calling God. The conclusion shows that religious call and answer are existential and theandric experiences, where one can work on askesis, the fidelity of thought, and mystical experience. Life in the Holy Spirit no longer distinguishes between call and answer for one who became a son of God by grace, faith, and good works.
On the Influence of Religious Assumptions in Statistical Methods Used in Science
Authors: Hunter Cornelius
Published: December 2020

For several centuries, statistical testing has been used to support evolutionary theories. Given the diverse origins and applications of these tests, it is remarkable how consistent they are. One common theme among these tests is that they appear to be founded on the logical fallacy of a false dichotomy. Is this true? It would be somewhat surprising if such diverse and historically important works are all guilty of the same naïve fallacy. Here, I explore these works and their historical context. I demonstrate that they are not logically fallacious, but instead incorporate and require a religious assumption about how a Creator would act. I conclude that this religious assumption and its influence on science should be considered in models of the interaction between science and religion.
The Sustainability Factor: How Much Do Pension Expenditures Improve in Spain?
Authors: Devesa Enrique , Devesa Mar , Dominguez-Fabián Inmaculada , Encinas Borja , Meneu Robert
Published: December 2020

The reform of 2013 represented a qualitative leap in the reform of the Spanish pension system. Unlike its predecessors, it introduced two automatic resetting mechanisms similar to those of other European countries. The first is the sustainability factor, scheduled to come into effect in 2019 but delayed until 2023, and its ultimate reversal cannot be ruled out. The objective of this study was to quantify the savings, or the lowest expenditure, that can be achieved in the Spanish public contributory pension system by applying it. These savings are measured in terms of cash—of annual expenditure—and in terms of accrual by calculating its present actuarial value. Combining these two methods is one of the contributions of this work. This work was only intended to analyze the impact of the Sustainability Factor, therefore, it did not take into account the impact of the Pension Revaluation Index, which is the second mechanism introduced in the reform of the pension to 2013. An ad hoc projection method was used, combining microdata from the Continuous Sample of Working Lives (MCVL), aggregate data from the pension system, the financial-actuarial projection method, and actuarial techniques. The diversity of the data used is the second contribution of this work. The application of the sustainability factor would improve the viability of the system, since the savings that could be achieved, measured in terms of GDP for each year, would be 1.029% by 2050; 1.094% in 2057, the maximum; and 1.026% in the last year of projection. In terms of the present actuarial value and as a function of annual GDP, in 2050, the savings would be 1.27%, 1.40% in 2044, the maximum, and in 2067 it would decrease to 0.98%.
Images of Roman Imperial Denarii: A Curated Data Set for the Evaluation of Computer Vision Algorithms Applied to Ancient Numismatics, and an Overview of Challenges in the Field
Authors: Arandjelović Ognjen , Zachariou Marios
Published: December 2020

Automatic ancient Roman coin analysis only recently emerged as a topic of computer science research. Nevertheless, owing to its ever-increasing popularity, the field is already reaching a certain degree of maturity, as witnessed by a substantial publication output in the last decade. At the same time, it is becoming evident that research progress is being limited by a somewhat veering direction of effort and the lack of a coherent framework which facilitates the acquisition and dissemination of robust, repeatable, and rigorous evidence. Thus, in the present article, we seek to address several associated challenges. To start with, (i) we provide a first overview and discussion of different challenges in the field, some of which have been scarcely investigated to date, and others which have hitherto been unrecognized and unaddressed. Secondly, (ii) we introduce the first data set, carefully curated and collected for the purpose of facilitating methodological evaluation of algorithms and, specifically, the effects of coin preservation grades on the performance of automatic methods. Indeed, until now, only one published work at all recognized the need for this kind of analysis, which, to any numismatist, would be a trivially obvious fact. We also discuss a wide range of considerations which had to be taken into account in collecting this corpus, explain our decisions, and describe its content in detail. Briefly, the data set comprises 100 different coin issues, all with multiple examples in Fine, Very Fine, and Extremely Fine conditions, giving a total of over 650 different specimens. These correspond to 44 issuing authorities and span the time period of approximately 300 years (from 27 BC until 244 AD). In summary, the present article should be an invaluable resource to researchers in the field, and we encourage the community to adopt the collected corpus, freely available for research purposes, as a standard evaluation benchmark.
How We Understand “Complexity” Makes a Difference: Lessons from Critical Systems Thinking and the Covid-19 Pandemic in the UK
Authors: Jackson Michael C.
Published: December 2020

Many authors have sought to summarize what they regard as the key features of “complexity”. Some concentrate on the complexity they see as existing in the world—on “ontological complexity”. Others highlight “cognitive complexity”—the complexity they see arising from the different interpretations of the world held by observers. Others recognize the added difficulties flowing from the interactions between “ontological” and “cognitive” complexity. Using the example of the Covid-19 pandemic in the UK, and the responses to it, the purpose of this paper is to show that the way we understand complexity makes a huge difference to how we respond to crises of this type. Inadequate conceptualizations of complexity lead to poor responses that can make matters worse. Different understandings of complexity are discussed and related to strategies proposed for combatting the pandemic. It is argued that a “critical systems thinking” approach to complexity provides the most appropriate understanding of the phenomenon and, at the same time, suggests which systems methodologies are best employed by decision makers in preparing for, and responding to, such crises.
A Machine Learning Based Classification Method for Customer Experience Survey Analysis
Authors: Markoulidakis Ioannis , Rallis Ioannis , Georgoulas Ioannis , Kopsiaftis George , Doulamis Anastasios , Doulamis Nikolaos
Published: December 2020

Customer Experience (CX) is monitored through market research surveys, based on metrics like the Net Promoter Score (NPS) and the customer satisfaction for certain experience attributes (e.g., call center, website, billing, service quality, tariff plan). The objective of companies is to maximize NPS through the improvement of the most important CX attributes. However, statistical analysis suggests that there is a lack of clear and accurate association between NPS and the CX attributes’ scores. In this paper, we address the aforementioned deficiency using a novel classification approach, which was developed based on logistic regression and tested with several state-of-the-art machine learning (ML) algorithms. The proposed method was applied on an extended data set from the telecommunication sector and the results were quite promising, showing a significant improvement in most statistical metrics.
UBE1a Suppresses Herpes Simplex Virus-1 Replication
Authors: Ikeda Marina , Ito Akihiro , Sekine Yuichi , Fujimuro Masahiro
Published: December 2020

Herpes simplex virus-1 (HSV-1) is the causative agent of cold sores, keratitis, meningitis, and encephalitis. HSV-1-encoded ICP5, the major capsid protein, is essential for capsid assembly during viral replication. Ubiquitination is a post-translational modification that plays a critical role in the regulation of cellular events such as proteasomal degradation, protein trafficking, and the antiviral response and viral events such as the establishment of infection and viral replication. Ub-activating enzyme (E1, also named UBE1) is involved in the first step in the ubiquitination. However, it is still unknown whether UBE1 contributes to viral infection or the cellular antiviral response. Here, we found that UBE1a suppressed HSV-1 replication and contributed to the antiviral response. The UBE1a inhibitor PYR-41 increased HSV-1 production. Immunofluorescence analysis revealed that UBE1a highly expressing cells presented low ICP5 expression, and vice versa. UBE1a inhibition by PYR-41 and shRNA increased ICP5 expression in HSV-1-infected cells. UBE1a reduced and retarded ICP5 protein expression, without affecting transcription of ICP5 mRNA or degradation of ICP5 protein. Additionally, UBE1a interacted with ICP27, and both partially co-localized at the Hsc70 foci/virus-induced chaperone-enriched (VICE) domains. PYR-41 reduced the co-localization of UBE1a and ICP27. Thus, our findings provide insights into the mechanism of UBE1a in the cellular response to viral infection.
IMU-838, a Developmental DHODH Inhibitor in Phase II for Autoimmune Disease, Shows Anti-SARS-CoV-2 and Broad-Spectrum Antiviral Efficacy In Vitro
Authors: Hahn Friedrich , Wangen Christina , Häge Sigrun , Peter Antonia Sophia , Dobler Gerhard , Hurst Brett , Julander Justin , Fuchs Jonas , Ruzsics Zsolt , Überla Klaus , Jäck Hans-Martin , Ptak Roger , Muehler Andreas , Gröppel Manfred , Vitt Daniel , Peelen Evelyn , Kohlhof Hella , Marschall Manfred
Published: December 2020

The ongoing pandemic spread of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) demands skillful strategies for novel drug development, drug repurposing and cotreatments, in particular focusing on existing candidates of host-directed antivirals (HDAs). The developmental drug IMU-838, currently being investigated in a phase 2b trial in patients suffering from autoimmune diseases, represents an inhibitor of human dihydroorotate dehydrogenase (DHODH) with a recently proven antiviral activity in vitro and in vivo. Here, we established an analysis system for assessing the antiviral potency of IMU-838 and DHODH-directed back-up drugs in cultured cell-based infection models. By the use of SARS-CoV-2-specific immunofluorescence, Western blot, in-cell ELISA, viral yield reduction and RT-qPCR methods, we demonstrated the following: (i) IMU-838 and back-ups show anti-SARS-CoV-2 activity at several levels of viral replication, i.e., protein production, double-strand RNA synthesis, and release of infectious virus; (ii) antiviral efficacy in Vero cells was demonstrated in a micromolar range (IMU-838 half-maximal effective concentration, EC50, of 7.6 ± 5.8 µM); (iii) anti-SARS-CoV-2 activity was distinct from cytotoxic effects (half-cytotoxic concentration, CC50, >100 µM); (iv) the drug in vitro potency was confirmed using several Vero lineages and human cells; (v) combination with remdesivir showed enhanced anti-SARS-CoV-2 activity; (vi) vidofludimus, the active determinant of IMU-838, exerted a broad-spectrum activity against a selection of major human pathogenic viruses. These findings strongly suggest that developmental DHODH inhibitors represent promising candidates for use as anti-SARS-CoV-2 therapeutics.
Advances in the Bioinformatics Knowledge of mRNA Polyadenylation in Baculovirus Genes
Authors: Peros Iván Gabriel , Cerrudo Carolina Susana , Pilloff Marcela Gabriela , Belaich Mariano Nicolás , Lozano Mario Enrique , Ghiringhelli Pablo Daniel
Published: December 2020

Baculoviruses are a group of insect viruses with large circular dsDNA genomes exploited in numerous biotechnological applications, such as the biological control of agricultural pests, the expression of recombinant proteins or the gene delivery of therapeutic sequences in mammals, among others. Their genomes encode between 80 and 200 proteins, of which 38 are shared by all reported species. Thanks to multi-omic studies, there is remarkable information about the baculoviral proteome and the temporality in the virus gene expression. This allows some functional elements of the genome to be very well described, such as promoters and open reading frames. However, less information is available about the transcription termination signals and, consequently, there are still imprecisions about what are the limits of the transcriptional units present in the baculovirus genomes and how is the processing of the 3′ end of viral mRNA. Regarding to this, in this review we provide an update about the characteristics of DNA signals involved in this process and we contribute to their correct prediction through an exhaustive analysis that involves bibliography information, data mining, RNA structure and a comprehensive study of the core gene 3′ ends from 180 baculovirus genomes.
First Evidence of Carp Edema Virus Infection of Koi Cyprinus carpio in Chiang Mai Province, Thailand
Authors: Pikulkaew Surachai , Phatwan Khathawat , Banlunara Wijit , Intanon Montira , Bernard John K.
Published: December 2020

The presence of carp edema virus (CEV) was confirmed in imported ornamental koi in Chiang Mai province, Thailand. The koi showed lethargy, loss of swimming activity, were lying at the bottom of the pond, and gasping at the water’s surface. Some clinical signs such as skin hemorrhages and ulcers, swelling of the primary gill lamella, and necrosis of gill tissue, presented. Clinical examination showed co-infection by opportunistic pathogens including Dactylogyrus sp., Gyrodactylus sp. and Saprolegnia sp. on the skin and gills. Histopathologically, the gill of infected fish showed severe necrosis of epithelial cells and infiltrating of eosinophilic granular cells. Electron microscope examination detected few numbers of virions were present in the cytoplasm of gill tissue which showed an electron dense core with surface membranes worn by surface globular units. Molecular detection of CEV DNA from gill samples of fish was performed by polymerase chain reaction (PCR) and confirmed by nested-PCR. Phylogenetic analyses revealed that CEV isolate had 99.8% homology with the CEV isolated from South Korea (KY946715) and Germany (KY550420), and was assigned to genogroup IIa. In conclusion, this report confirmed the presence of CEV infection of koi Cyprinus carpio in Chiang Mai province, Thailand using pathological and molecular approaches.
Intramuscular and Intradermal Electroporation of HIV-1 PENNVAX-GP® DNA Vaccine and IL-12 Is Safe, Tolerable, Acceptable in Healthy Adults
Authors: Edupuganti Srilatha , C. De Rosa Stephen , Elizaga Marnie , Lu Yiwen , Han Xue , Huang Yunda , Swann Edith , Polakowski Laura , A. Kalams Spyros , Keefer Michael , Maenza Janine , C. Wise Megan , Yan Jian , Morrow Matthew , Khan Amir , Boyer Jean , Humeau Laurent , White Scott , Sardesai Niranjan , Bagarazzi Mark , Gilbert Peter , Kublin James , Corey Lawrence , Weiner David ,   on behalf of the HVTN 098 Study Team,   the NIAID-Funded HIV Vaccine Trials Network
Published: December 2020

Background: Several techniques are under investigation to improve the immunogenicity of HIV-1 DNA vaccine candidates. DNA vaccines are advantageous due to their ease of design, expression of multiple antigens, and safety. Methods: The HVTN 098 trial assessed the PENNVAX®-GP DNA vaccine (encoding HIV env, gag, pol) administered with or without plasmid IL-12 at 0-, 1-, 3-, and 6-month timepoints via intradermal (ID) or intramuscular (IM) electroporation (EP) in healthy, adult participants. We report on safety, tolerability, and acceptability. Results: HVTN 098 enrolled 94 participants: 85 received PENNVAX®-GP and nine received placebo. Visual analog scale (VAS) pain scores immediately after each vaccination were lower in the ID/EP than in the IM/EP group (medians 4.1–4.6 vs. 6–6.5, p < 0.01). IM/EP participants reported greater pain and/or tenderness at the injection site. Most ID/EP participants had skin lesions such as scabs/eschars, scars, and pigmentation changes, which resolved within 6 months in 51% of participants (24/55). Eighty-two percent of IM/EP and 92% of ID/EP participant survey responses showed acceptable levels of discomfort. Conclusions: ID/EP and IM/EP are distinct experiences; however, HIV-1 DNA vaccination by either route was safe, tolerable and acceptable by most study participants.
Recombinant Enterovirus 71 Viral Protein 1 Fused to a Truncated Newcastle Disease Virus NP (NPt) Carrier Protein
Authors: Mustafa Suhaili , Abd-Aziz Noraini , Saw Wuan-Ting , Liew Sien-Yei , Yusoff Khatijah , Shafee Norazizah
Published: December 2020

Enterovirus 71 (EV71) is the major causative agent in hand, foot, and mouth disease (HFMD), and it mainly infects children worldwide. Despite the risk, there is no effective vaccine available for this disease. Hence, a recombinant protein construct of truncated nucleocapsid protein viral protein 1 (NPt-VP1198–297), which is capable of inducing neutralizing antibody against EV71, was evaluated in a mouse model. Truncated nucleocapsid protein Newcastle disease virus that was used as immunological carrier fused to VP1 of EV71 as antigen. The recombinant plasmid carrying corresponding genes was constructed by recombinant DNA technology and the corresponding protein was produced in Escherichia coli expression system. The recombinant NPt-VP1198–297 protein had elicited neutralizing antibodies against EV71 with the titer of 1:16, and this result is higher than the titer that is elicited by VP1 protein alone (1:8). It was shown that NPt containing immunogenic epitope(s) of VP1 was capable of inducing a greater functional immune response when compared to full-length VP1 protein alone. It was capable to carry larger polypeptide compared to full-length NP protein. The current study also proved that NPt-VP1198–297 protein can be abundantly produced in recombinant protein form by E. coli expression system. The findings from this study support the importance of neutralizing antibodies in EV71 infection and highlight the potential of the recombinant NPt-VP1198–297 protein as EV71 vaccine.
Serum Anti-HPV Antibody Titer as a Marker of Vaccine Effectiveness in Males with Genital Infection
Authors: De Toni Luca , Muscianisi Francesco , Corsini Christian , Ghezzi Marco , Di Nisio Andrea , Foresta Carlo , Garolla Andrea
Published: December 2020

Introduction: Persistent human papillomavirus (HPV) semen infection is increasingly associated with male infertility. Adjuvant HPV vaccination is suggested to reduce the time to clearance and the disease relapse in males with persistent HPV semen infection. However, only a sub-population of patients show a clinical benefit from adjuvant vaccination. Here, we aimed to address the effectiveness rate of HPV adjuvant vaccination in males with genital tract infection and the possible prognostic markers of healing. Methods: Clinical records from 379 patients with persistent seminal HPV detection, all receiving HPV adjuvant vaccination, were considered. Clinical data, including genital HPV-DNA assessment by INNO-LiPA genotyping, semen HPV-DNA analysis by FISH analysis and serum antibody titer, were collected at basal (T0) and after 6 months (T1) since the vaccination cycle ended. Results: Clearance of genital HPV-DNA was recorded in 326 (86%) patients. Serum HPV-antibody titer at T1 was the most important prognostic factor associated with HPV-DNA clearance. A serum antibody titer equal to or greater than the threshold value 1:125, obtained by ROC curve analysis, was prognostic of healing. Conclusions: Anti-HPV antibody represents a suitable marker of adequate immune response to HPV vaccination in patients with genital infection.
Safer Enteral Syringes
Authors: Guenter Peggi , Lyman Beth
Published: December 2020

A common example of wrong route errors is when parenteral syringes are used to draw up medications that are then inadvertently administered into an intravenous line rather than an enteral feeding tube. With the transition to safer enteral syringes with ENFit connectors in the United States, it is critical that nurses use the correct enteral syringes to prepare and administer medications. These wrong route errors are still happening and the need to move to the safer connectors and use them appropriately is vital.
Sepsis in the Time of COVID-19
Authors: Allen Caitlyn
Published: December 2020

This year, almost 2 million adults will contract this condition, and 270,000 will die as a result. Not COVID-19, sepsis. Cait Allen sat down with Dr. David F. Gaieski, emergency medicine physician and director of Emergency Critical Care at Jefferson Health, to understand what sepsis is, its relationship with COVID-19, and how we can—and must—prevent both.
STUDY OF MECHANOACTIVATION EFFECTIVENESS OF GRANITE SCREENING IN MILLS OF VARIOUS TYPES WHEN SYNTHESIS OF NANOSTRUCTURED BINDER
Authors: Evtushenko Evgeniy , Cherevatova A. , Kozhuhova N. , Osadchaya M. , Starostina Irina , Kozhuhova M.
Published: December 2020

In this paper, the possibility of obtaining nanostructured binders (NB) based on acidic igneous intrusive rocks is considered by the example of screening out granite using milling units of different types, followed by an assessment of the degree of effectiveness of their application for this type of aluminosilicate raw material. A comparative analysis of the degree of mechanoactiva-tion impact of them is carried out. A comparative analysis of the microstructure of samples of granite screening, crushed in three different milling units has been realized. It has been found that the type of milling unit significantly affects not only the degree of dispersion and the nature of the particle size distribution of particles, but also on morphological features of them. It was revealed that the most effective milling unit for obtaining (synthesizing) a binder system from granite screening is a ball mill, as the content of nano- and micro-level particles in this system are maximum, which ultimately determines its quality as a binder. The analysis of the structural, morphological, and strength characteristics of NB obtained from the granite screening in a ball mill, which is following similar data on a quartz NB and allow us to speak about the effectiveness of the use of a ball mill is a grinding machine and granite screening as a raw component for the synthesis of NB.
HISTORY OF HOLY TRINITY CATHEDRAL (YAKUTSK) IN CERAMIC BRICK
Authors: POTAPOVA A. , BELOLYUBSKAYA S. , Egorova A.
Published: December 2020

The study of the history of ceramic brick production in Yakutsk and the chronology of changing its nominal size is clearly seen on the example of stone buildings of the pre-revolutionary period, starting from the 18th century. Many architectural buildings made of ceramic brick have retained their integrity, uniqueness and exclusivity. Based on literary sources and archival documents, the main stages of the construction of the Trinity Cathedral (from 1708 to 1901) are traced, during which the unique architectural appearance of the first stone church in the city of Yakutsk is formed. Holy Trinity Cathedral in Yakutsk and recreating its historical appearance, bricks of the 18th and 19th centuries are selected to determine the durability of brickwork for the possibility of restoring the ancient architectural monument and their physical and mechanical properties are determined. The initial results of the survey of the building with a description of the structural features of the construction of brick walls and floors, the results of the study of the physical and mechanical properties of ceramic brick samples of different centuries are given. It is determined that ceramic bricks of the 18th century have a smaller grade in strength than bricks of the 19th century, but they are in good condition, which allows the restoration work. This study is another attempt to comprehend and understand the secrets of the masters, systematize and restore the chronology of the monument's history.
EXPERIÊNCIA DOCENTE COM APRENDIZAGEM SIGNIFICATIVA EM ETEC
Authors: Sanches Daniela Brugnaro Massari , Uemura Denise Tieko , Sguassábia Fernanda Cassiolato Marti , Garcia Samara Pereira
Published: December 2020

EXPERIÊNCIA DOCENTE COM APRENDIZAGEM SIGNIFICATIVA EM ETEC
AS ESCOLAS DO CENTRO PAULA SOUZA NAS PALAVRAS DE SEUS TRABALHADORES EXPRESSÕES DOCENTES EM MEIO A EXPERIÊNCIAS EDUCATIVAS
Authors: Oliveira Junior Wenceslao Machado de
Published: December 2020

AS ESCOLAS DO CENTRO PAULA SOUZA NAS PALAVRAS DE SEUS TRABALHADORES EXPRESSÕES DOCENTES EM MEIO A EXPERIÊNCIAS EDUCATIVAS
O TRABALHO DE CAMPO NA FORMAÇÃO DO TÉCNICO EM MEIO AMBIENTE
Authors: Oliveira João Victor Pavesi de
Published: December 2020

O TRABALHO DE CAMPO NA FORMAÇÃO DO TÉCNICO EM MEIO AMBIENTE
COMO É SER ALUNO DO ENSINO MÉDIO E ETIM? EXPERIÊNCIAS, AVENTURAS E SITUAÇÕES DOS ALUNOS NAS ETEC’S
Authors: Antonio Davi Gutierrez
Published: December 2020

COMO É SER ALUNO DO ENSINO MÉDIO E ETIM? EXPERIÊNCIAS, AVENTURAS E SITUAÇÕES DOS ALUNOS NAS ETEC’S
PERAN ORANG TUA DALAM MEMBERIKAN PENDIDIKAN SEKS PADA ANAK USIA 6-13 TAHUN DI DESA SUNGAI KUYUNG KECAMATAN PANCUNG SOAL
Authors:   STKIP Pesisir Selatan, Ramadhani Ramadhani , Efendi Efendi ,   STKIP Pesisir Selatan
Published: December 2020

The purpose of this study is to illustrate the efforts made by parents in providing sex education to children aged 6-13 years. To explain what form of child questions about sex and to explain the obstacles faced by parents in providing sex education to children aged 6-13 years in Sungai Kuyung Kecamatan Pancung Soal Sub-District. This research is field research with descriptive qualitative methods. In the determination of the sample the author uses snowball sampling. The results of the research that has been conducted in Sungai Kuyung River Village are parents trying to provide sex education to the child and answer the child's questions about sex. Here parents play a role in teaching child sex abuse by applying when entering the parent's room by asking permission before entering, teaching how to look at the situation seen by the child. Then parents also avoid the child from free sex by separating the bed between the parent and brother or sister. Parents also explain about sex to their child as much as they can.
PENGARUH SUPERVISE KLINIS DAN SUPERVISE AKADEMIK KEPALA SEKOLAH TERHADAP KINERJA GURU DI SMP NEGERI 3 RAMBANG DANGKU KABUPATEN MUARA ENIM
Authors:   Pascasarjana, Universitas PGRI Palembang, Tamar Oktarina , Arafat Yasir , Mulyadi Mulyadi
Published: December 2020

his study describes the effect of clinical supervision and academic supervision of principals on teacher performance in state junior high school 3 Rambang Dangku Muara Enim Regency. This study uses quantitative methods. Techniques and data collection procedures through questionnaires, observation, and documentation. Data were analyzed using multiple linear regression. Based on the data analysis, the conclusion is 1) there is a clue to clinical supervision of teacher performance, meaning that clinical supervision can renew teacher performance. 2) There is an effect of principal academic supervision on teacher performance. 3) there are clinical supervision and academic supervision of principals on teacher performance, thus teaching skills and work motivation collectively on teacher performance. Clinical supervision and academic supervision of principals are able to affect teacher performance with a contribution of 24.1%
Особливості державної політики регулювання медичних послуг в Україні
Authors: Andriyash Victoria , Kozlova Liudmyla , Malikina Oksana , Polyakova Elena
Published: December 2020

У статті досліджено теоретичні та практичні основи державної політики щодо регулювання медичних послуг в Україні, що потребує підвищення рівня надання зазначених послуг та більш прозорої діяльності з надання таких послуг в умовах тривалого реформування в нашій країні. Авторами висвітлені особливості державного регулювання ринку медичних послуг, котрий перебуває у стані тривалого розвитку, котрий потребує жорсткого законодавчого регулювання для подальшого ефективного розвитку. Відзначено існування національних особливостей публічно-правового регулювання ринку медичних послуг в різних країнах, представлені практично діаметрально протилежними підходами і принципами. Здійснено аналіз державної політики України в галузі охорони здоров’я в якості організаційної та нормативної системи владних дій та управлінських рішень й основного регулятора здоров’я громадян. У статті сформульовано визначення державної політики регулювання медичних послуг з урахуванням специфіки медичної системи, окреслено проблеми в сфері організації ефективного державного регулювання медичних послуг та їх стандартизації, надані окремі рекомендації для створення ефективних механізмів державного впливу на ринок медичних послуг.
Управління освітою: зміна управлінської вертикалі
Authors: Sahuichenko Valentyna , Volokitina Nataliia
Published: December 2020

У статті акцентовано на необхідності проведення освітніх реформ для забезпечення якісної освіти, важливості зміни управлінської складової з поступовим переходом від державного управління до державно-громадського, залучення місцевої громади до підтримки освітніх реформ.
Проаналізовано розуміння керівниками навчальних закладів необхідності впровадження освітніх реформ, їх своєчасності, теоретико-методологічного обґрунтування реформування освітніх інституцій, ставлення до задекларованих процесів реформування за паритетним поділенням повноважень між державою і місцевою громадою.
Доведено, що практичні результати освітніх реформ, зокрема в закладах загальної середньої освіти залежать від управлінських компетенцій керівників цих закладів, які включають знання з питань теоретичної рефлексії досвіду про освіту, управління, виховання; з питань філософсько-антропологічного обґрунтування коеволюції освітніх інституцій і сучасного суспільства; культурологічних аспектів; синергетичних процесів; міждисциплінарного підходу; національної культури і інтеграційних процесів в освіті; входження до європейського освітнього простору.
У висновках за результатами проведеного експерименту підсумовано, що є причинами неякісної освіти, серед яких: непрофесійність наукових і науково-педагогічних працівників; слабке матеріально-технічне оснащення; відсутність сучасних компьютерів; незацікавленість учнів і студентів у навчанні; перевантаження учнів і педагогів; відсутність варіантів для індивідуальної освітньої траєкторіїґ; відсутність перспектив подальшого гідного працевлаштуванн та запропоновано шляхи для задіяння ефективних механізмів з питань підвищення якості освіти на курсах підвищення кваліфікації в регіональних інститутах післядипломної педагогічної освіти.
Державне управління процесами міжнародного співробітництва України в сфері інформаційних відносин
Authors: Shtyrov Oleksandr , Verba Svitlana
Published: December 2020

У статті розглядаються особливості державного управління процесами міжнародного співробітництва України у сфері інформаційних відносин, зокрема характеристика процесів міжнародного співробітництва у сфері інформаційних відносин, аналіз державної політики у сфері міжнародних відносин до шляхів удосконалення механізму державного управління сферою інформаційних відносин.
Міжнародне співробітництво у сфері інформаційних відносин відбувається в процесі міжнародної інформаційної діяльності між двома або більше країнами на світовому ринку послуг. Основою є надання громадянам, державним органам, підприємствам, установам та організаціям офіційної інформації (у документальному вигляді або публічно оголошеною) про різні події та явища на міжнародному рівні. Це може бути, наприклад, зовнішньополітична діяльність України, події в інших країнах, а також цілеспрямоване розповсюдження за межі України інформації про нашу країну.
Міжнародні інформаційні відносини є доволі новим поняттям на світовому просторі, але за досить короткий час стали важливими та впливовими на загальний економічний, політичний, соціальний стан країн-учасниць.
Міжнародна просвітницька діяльність полягає у наданні всім учасникам (наприклад, громадянам, державним органам, бізнесу) інформації про зовнішньополітичну діяльність України, яка офіційно задокументована або публічно оприлюднена, а також цілеспрямовано поширювати вичерпну інформацію за межами країни про Україну через, наприклад, засоби масової інформації, державні установи та громадян.
Встановлено, що в умовах сучасного стану розвитку суспільного розвитку, а також враховуючи ситуацію українського та міжнародного інформаційного простору, необхідно посилити роль держави у забезпеченні доступу громадян України до оперативної інформації та гарантувати захист і достовірність національного інформаційного простору.
Європейський досвід у сфері взаємодії держави та інститутів громадянського суспільства
Authors: Shtyrov Alexander , Yemelyanov Volodymyr
Published: December 2020

У статті розглянуто питання взаємодії держави та громадянського суспільства в контексті досвіду європейських країн, визначено форми такої взаємодії, можливість застосування європейського досвіду в Україні.
Вказано на те що розбудова демократичної, правової, соціальної держави є ключовим та важливим пріоритетом, а реалізація надважливого стратегічного курсу на інтеграцію з Європейським Союзом – є кінцевою метою цього процесу, цьому сприяє також забезпечення всебічного входження України у європейський політичний, економічний і правовий простір.
Розкрито взаємодію інститутів громадянського суспільства і держави яка має формуватися на європейських загальноприйнятих засадах, таких як взаємна співпраця, партнерство.
Показано що держава формуючи інституційно-правове середовище всебічно сприяє взаємодії та розвитку громадянського суспільства.
Зазначено що сучасною тенденцією у відносинах державних органів та інститутів громадянського суспільства є трансформація системи грантів у систему контрактів, яка посилює скорочення фінансової залежності громадянського суспільства від державної влади.
Констатовано що загалом, у країнах ЄС поступово відмовляються від формату засідань консультативно-дорадчих органів на користь онлайн-консультацій з громадськістю.
Merayakan Persahabatan Yang Memerdekakan Refleksi Atas Pandangan Odo Casel Tentang Misteri Kehadiran Karya Keselamatan Dalam Liturgi
Authors: Manik Pius
Published: December 2020

Scripture shows that liturgy is the basic reason why GOD saved his people so that God’s chosen people might freely worship God (Ex 4:23) in spirit and truth’ (Jn 4:24). Liturgy becomes a mediator and a moment for the real presence of God’s saving deed. The relationship between God and His/Her people is drawn into a reality in our liturgy.  Odo Casel reflects on our relationship with God is not a mere speculative idea but it is an objective reality that happens in our daily life. The Encyclic Mediator Dei emphasizes the Divine aspect of God’s grace in the history of salvation that restored our relationship with God.
Mampukah Citra Merek, Fasilitas dan Kepercayaan Meningkatkan Kepuasan Pengunjung Taman Hewan Kota Pematangsiantar
Authors: Sinaga Onita Sari , Putri Debi Eka , Banjarnahor Astri Rumondang , Halim Fitria , Halim Fitria , Sudirman Acai
Published: December 2020

The manifestation of customer satisfaction can be seen from the pattern of consumer behavior on a product of goods and services. Many factors can influence consumer behavior, including product quality, price offered, promotion mechanism presented, ease of seeking purchase places, and many other factors that can influence it. This study aimed to examine the effect of brand image, facilities, and consumer trust on customer satisfaction, both partially and simultaneously. This study used a quantitative method. The data were obtained from the distribution of questionnaires to 321 respondents who were the sample of the population studied. The data analysis technique used to test the hypothesis was multiple regression analysis which included the t-test, F-test, and the coefficient of determination test. The results of the study with the t-test model explained that: the brand image had an effect on customer satisfaction, facilities had an effect on customer satisfaction, consumer trust had an effect on customer satisfaction. Furthermore, the results of the simultaneous test (F-test) stated that brand image, facilities, and consumer trust had a significant effect on customer satisfaction. The coefficient of determination test explained that customer satisfaction n was influenced by brand image, facilities, and consumer trust by 71.6% and the remaining 28.4% was influenced by other factors not examined in this study.