bluuebunny/crossref_metadata_2025 huggingface dataset:
Enterprise Design Thinking: An Investigation on User-Centered Design Processes in Large Corporations
Authors: Kwon Jieun , Choi Younghyun , Hwang Yura
Published: July 2021

Design thinking (DT) is considered a “human-centered” or “user-needs” process that leads to business innovation. Promising practices of DT application in real life have gained popularity in business, such as IBM’s the loop, IDEO’s 3I model, Design Council’s double diamond model, and Stanford’s d.school model. However, many existing studies have targeted DT models in a small group setting, such as a startup environment or a small group in an educational institution. The primary purpose of this paper was to investigate how large corporate environments that have multiple departments with a waterfall culture define and apply DT in real use. The first part of this paper introduces the most popular DT models used in the industry and some insights into their use in the field; the second part examines the experiences of 20 professionals who have experience with DT in the large organizational environment through in-depth interviews. As a result, this paper offers three major insights regarding the use of DT in the field. The third part of this study suggests an appropriate enterprise DT model for large corporations based on the problem-solving activities already undertaken by employees. This study is important as the first step toward understanding DT implementation in large corporations.
Diagnostic Accuracy of B-Mode- and Contrast-Enhanced Ultrasound in Differentiating Malignant from Benign Pleural Effusions
Authors: Safai Zadeh Ehsan , Weide Johanna , Dietrich Christoph Frank , Trenker Corinna , Koczulla Andreas Rembert , Görg Christian
Published: July 2021

Purpose: To evaluate the value of CEUS in differentiating malignant from benign pleural effusions (PEs). Methods: From 2008 to 2017, 83 patients with PEs of unknown cause were examined using B-mode thoracic ultrasound (B-TUS), CEUS, and cytological examination. The extent of enhancement of the pleural thickening, the presence of enhancement of septa or a solid mass within the PE, and the homogeneity of the enhancement in the associated lung consolidation, were examined. Subsequently, the diagnostic value of cytology, B-TUS, and CEUS in differentiating malignant from benign PEs was determined. Results: With CEUS, markedly enhanced pleural thickening and inhomogeneous enhanced lung consolidation were significantly more frequently associated with malignancy (p < 0.05). In the subgroup analysis, the use of CEUS increased the sensitivity from 69.2 to 92.3 in patients with initial negative cytology but clinical suspicion of malignant PE; it also increased the specificity from 63.0 to 90.0, the positive predictive value from 69.2 to 92.3, the negative predictive value from 63.0 to 90.0, and the diagnostic accuracy from 66.7 to 87.5, in the evaluation of PE malignancy. Conclusion: The use of clinically based B-TUS and CEUS as a complementary method to cytological evaluation may be beneficial for evaluating a PE of unknown cause. CEUS patterns of enhanced pleural thickening and inhomogeneous enhanced lung consolidation may suggest a malignant PE.
Evaluation of Integrin αvβ3 Expression in Murine Xenograft Models: [68Ga]Ga-DOTA-C(RGDfK) PET Study with Immunohistochemical Confirmation
Authors: Mitsuyuki Kosuke , Watabe Tadashi , Naka Sadahiro , Liu Yuwei , Tatsumi Mitsuaki , Shimosegawa Eku , Kato Hiroki
Published: July 2021

Tumor blood flow (TBF) is related to drug delivery and hypoxia, both of which can impact the efficacy of anti-cancer therapies. Although integrin αvβ3 expression is related to tumor angiogenesis, it remains unclear whether the degree of angiogenesis affects TBF. This study aimed to evaluate the expression of integrin αvβ3 in mouse tumor models using [68Ga]Ga-DOTA-c(RGDfK) peptide positron emission tomography (PET) and immunohistochemical staining. PET studies were conducted using mouse C6 glioma models and MIA PaCa-2 (n = 6 each). The [68Ga]Ga-DOTA-c(RGDfK) peptide was injected via the tail vein (2.17 ± 0.28 MBq), and 10 min static PET scans were performed. Immunohistochemical analysis was conducted using an integrin αVβ3 antibody. [68Ga]Ga-DOTA-c(RGDfK) peptide PET revealed higher uptake of the radiotracer in C6 gliomas than in MIA PaCa-2 tumors. The mean standardized uptake value was significantly higher in C6 gliomas (0.35 ± 0.058) than in MIA PaCa-2 tumors (0.17 ± 0.045). Histological analysis revealed intense integrin αVβ3 expression in the C6 gliomas, whereas the MIA PaCa-2 tumors had low expression levels. This study showed that the expression of integrin αvβ3 can be differentiated by the [68Ga]Ga-DOTA-c(RGDfK) peptide, suggesting the potential applicability of this peptide in the evaluation of the relationship between angiogenesis and TBF.
Laying the Groundwork to Investigate Diversity of Life Sciences Reading Lists in Higher Education and Its Link to Awarding Gaps
Authors: Taylor Maria , Hung Jacob , Che Thi Elena , Akinbosede Daniel , Petherick Katy J. , Pranjol Md Zahidul I.
Published: July 2021

This study presents a case for decolonising the life sciences curriculum to improve representation of the Black, Asian, and Minority Ethnic (BAME) scholars—a step in eliminating the race “awarding gap”. Here, we investigated diversity among authors in terms of ethnicity and gender of reading lists at the School of Life Sciences, University of Sussex. We show that the reading lists are not diverse and do not represent the demography of the student body. For instance, a disproportionately high number of authors in the reading lists are white 83.40 ± 5.70% (n = 977 authors), male 75.90 ± 5.40% (n = 878 authors), and of European descent. Additionally, our analysis of the geographical locations of publications reveals that a significantly high number of our materials stem from the USA or the UK, whereas the second highest global output of scientific literature (after the USA) comes from China, which is only featured in 1.02% of the reading list. Moreover, we constructively provide potential solutions to decolonise the curriculum of the University of Sussex’s School of Life Sciences by diversifying their reading lists. This study should help to establish a foundation, along with other work that is being conducted, to address the BAME awarding gap and to better showcase the work of women and ethnically underrepresented scientists in history and in modern day.
Privacy Preservation in Resource-Constrained IoT Devices Using Blockchain—A Survey
Authors: Iftikhar Zainab , Javed Yasir , Zaidi Syed Yawar Abbas , Shah Munam Ali , Iqbal Khan Zafar , Mussadiq Shafaq , Abbasi Kamran
Published: July 2021

With opportunities brought by Internet of Things (IoT), it is quite a challenge to assure privacy preservation when a huge number of resource-constrained distributed devices is involved. Blockchain has become popular for its benefits, including decentralization, persistence, immutability, auditability and consensus. With the implementation of blockchain in IoT, the benefits provided by blockchain can be derived in order to make IoT more efficient and maintain trust. In this paper, we discuss some applications of IoT in different fields and privacy-related issues faced by IoT in resource-constrained devices. We discuss some applications of blockchain in vast majority of areas, and the opportunities it brings to resolve IoT privacy limitations. We, then, survey different researches based on the implementation of blockchain in IoT. The goal of this paper is to survey recent researches based on the implementation of blockchain in IoT for privacy preservation. After analyzing the recent solutions, we see that the blockchain is an optimal way for preventing identity disclosure, monitoring, and providing tracking in IoT.
Experimental Study of a Lab-Scale Organic Rankine Cycle System for Heat and Water Recovery from Flue Gas in Thermal Power Plants
Authors: Kim Young-Min , Negash Assmelash , Shamsi Syed Safeer Mehdi , Shin Dong-Gil , Cho Gyubaek
Published: July 2021

Fossil fuel power plants can cause numerous environmental issues, owing to exhaust emissions and substantial water consumption. In a thermal power plant, heat and water recovery from flue gas can reduce CO2 emissions and water demand. High-humidity flue gas averts the diffusion of pollutants, enhances the secondary transformation of air pollutants, and leads to smog weather; hence, water recovery from flue gas can also help to lessen the incidence of white plumes and smog near and around the power plant. In this study, a lab-scale system for heat and water recovery from flue gas was tested. The flue gas was initially cooled by an organic Rankine cycle (ORC) system to produce power. This gas was further cooled by an aftercooler, using the same working fluid to condense the water and condensable particulate matter in the flue gas. The ORC system can produce approximately 220 W of additional power from flue gas at 140 °C, with a thermal efficiency of 10%. By cooling the flue gas below 30–40 °C, the aftercooler can recover 60% of the water in it.
Recent Advances in Nondestructive Evaluation of Wood: In-Forest Wood Quality Assessments
Authors: Wang Xiping
Published: July 2021

Efficient wood production and utilization requires knowing the wood quality attributes of forest resources relevant to various end uses, prescribing appropriate silvicultural treatments that positively influence wood quality, and then, at the time of harvesting, sorting and allocating standing timbers to the most appropriate markets [...]
Design of Chitosan and Alginate Emulsion-Based Formulations for the Production of Monolayer Crosslinked Edible Films and Coatings
Authors: Vieira Tiago M. , Moldão-Martins Margarida , Alves Vítor D.
Published: July 2021

This study aimed to develop edible monolayer emulsion-based barriers with polysaccharides as film-forming components (chitosan and sodium alginate), soy lecithin as a surfactant and olive oil as a hydrophobic barrier. Monolayer barriers in the form of films were prepared by casting filmogenic emulsions composed of 2% w/v chitosan (dissolved in lactic acid 1% v/v) or 1% w/v sodium alginate, with different lipid contents (25, 50 and 100% w/w biopolymer basis) and different surfactant concentrations (5, 10 and 25% w/w, lipid basis). Glycerol was used as a plasticizer (25 % w/w, biopolymer basis). After the emulsion drying process, the obtained stand-alone films were sprayed with a crosslinking solution, achieving an optimized crosslinker content of 3.2 mgCa2+/cm2 alginate film and 4 mg tripolyphosphate/cm2 chitosan film. The effect of oil and lecithin contents, as well the presence of crosslinking agents, on the film’s water vapour permeability (WVP), water vapour sorption capacity, mechanical properties and colour parameters, was evaluated. The results have shown that the lowest WVP values were obtained with formulations containing 25% lipid and 25% surfactant for chitosan films, and 100% lipid and 25% surfactant for alginate films. The application of the crosslinking agents decreased even further the WVP, especially for chitosan films (by 30%). Crosslinking also increased films’ resistance to deformation under tensile tests. Overall, the films developed present a good potential as polysaccharide-based barriers with increased resistance to water, which envisages the use of the designed formulations to produce either edible/biodegradable films or edible coatings.
Ecklonia cava Extract Exerts Anti-Inflammatory Effect in Human Gingival Fibroblasts and Chronic Periodontitis Animal Model by Suppression of Pro-Inflammatory Cytokines and Chemokines
Authors: Jung Jae-In , Kim Seonyoung , Baek Seung-Min , Choi Soo-Im , Kim Gun-Hee , Imm Jee-Young
Published: July 2021

Periodontitis is one of the most common chronic inflammatory diseases. The anti-inflammatory effect of the extract from brown algae Ecklonia cava was analyzed in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGF-1), the most abundant cells in gingival tissue. The gene expressions of cyclooxygenase-2 and interleukin-6 were decreased by 78 and 50%, respectively, at 100 μg/mL Ecklonia cava extract (ECE) treatment. The gene expressions of matrix metalloproteases (MMP-2 and MMP-8) and chemokines (macrophage inflammatory protein 1-alpha and stromal cell-derived factor 1) were also significantly down-regulated by ECE treatment (p < 0.05). The increased reactive oxygen species (ROS) production in HGF-1 cells by LPS stimulation was decreased by 30% at 100 μg/mL ECE treatment. The mitogen-activated protein kinase pathway and the nuclear factor-kappa B (NF-κB) signal activated by ROS were suppressed by ECE in a dose-dependent manner. ECE treatment (400 mg/kg, 8 weeks) significantly improved alveolar bone resorption in the ligature-induced chronic periodontitis rat model. ECE supplementation also lowered elevated mRNA expression of the receptor activator of nuclear factor-kappa B (RANKL)/osteoprotegerin (OPG) in the gingival tissue (p < 0.05). Therefore, ECE mitigated gingival tissue destruction and bone resorption associated with chronic periodontitis condition.
Constructing Ableism
Authors: Jenkins Stephanie
Published: July 2021

This essay builds upon research in disability studies through the extension of Garland-Thomson’s figure of the normate. I argue that biopower, through the disciplinary normalization of individual bodies and the biopolitics of populations, in the nineteenth-century United States produced the normate citizen as a white, able-bodied man. The normate citizen developed with the new political technology of power that emerged with the transition from sovereign power to biopower. I focus on the disciplinary normalization of bodies and the role of industrial capitalism in the construction of able-bodied norms. I argue that the medical model of disability is produced through a dual process of incorporation: the production of corporeal individuals and the localization of illness in the body.
NGS Analysis of Liquid Biopsy (LB) and Formalin-Fixed Paraffin-Embedded (FFPE) Melanoma Samples Using Oncomine™ Pan-Cancer Cell-Free Assay
Authors: Olbryt Magdalena , Rajczykowski Marcin , Bal Wiesław , Fiszer-Kierzkowska Anna , Cortez Alexander Jorge , Mazur Magdalena , Suwiński Rafał , Widłak Wiesława
Published: July 2021

Next-generation sequencing (NGS) in liquid biopsies may contribute to the diagnosis, monitoring, and personalized therapy of cancer through the real-time detection of a tumor’s genetic profile. There are a few NGS platforms offering high-sensitivity sequencing of cell-free DNA (cfDNA) samples. The aim of this study was to evaluate the Ion AmpliSeq HD Technology for targeted sequencing of tumor and liquid biopsy samples from patients with fourth-stage melanoma. Sequencing of 30 samples (FFPE tumor and liquid biopsy) derived from 14 patients using the Oncomine™ Pan-Cancer Cell-Free Assay was performed. The analysis revealed high concordance between the qPCR and NGS results of the BRAF mutation in FFPE samples (91%), as well as between the FFPE and liquid biopsy samples (91%). The plasma-tumor concordance of the non-BRAF mutations was 28%. A total of 17 pathogenic variants in 14 genes (from 52-gene panel), including TP53, CTNNB1, CCND1, MET, MAP2K1, and GNAS, were identified, with the CTNNB1S45F variant being the most frequent. A positive correlation between the LDH level and cfDNA concentration as well as negative correlation between the LDH level and time to progression was confirmed in a 22-patient cohort. The analysis showed both the potential and limitations of liquid biopsy genetic profiling using HD technology and the Ion Torrent platform.
Nuclear Organization during Hepatogenesis in Zebrafish Requires Uhrf1
Authors: Madakashira Bhavani P. , Zhang Chi , Macchi Filippo , Magnani Elena , Sadler Kirsten C.
Published: July 2021

Acquisition of cellular fate during development is initiated and maintained by well-coordinated patterns of gene expression that are dictated by the epigenetic landscape and genome organization in the nucleus. While the epigenetic marks that mediate developmental gene expression patterns during organogenesis have been well studied, less is known about how epigenetic marks influence nuclear organization during development. This study examines the relationship between nuclear structure, chromatin accessibility, DNA methylation, and gene expression during hepatic outgrowth in zebrafish larvae. We investigate the relationship between these features using mutants that lack DNA methylation. Hepatocyte nuclear morphology was established coincident with hepatocyte differentiation at 80 h post-fertilization (hpf), and nuclear shape and size continued to change until the conclusion of outgrowth and morphogenesis at 120 hpf. Integrating ATAC-Seq analysis with DNA methylation profiling of zebrafish livers at 120 hpf showed that closed and highly methylated chromatin occupies most transposable elements and that open chromatin correlated with gene expression. DNA hypomethylation, due to mutation of genes encoding ubiquitin-like, containing PHD and RING Finger Domains 1 (uhrf1) and DNA methyltransferase (dnmt1), did not block hepatocyte differentiation, but had dramatic effects on nuclear organization. Hepatocytes in uhrf1 mutants have large, deformed nuclei with multiple nucleoli, downregulation of nucleolar genes, and a complete lack of the nuclear lamina. Loss of lamin B2 staining was phenocopied by dnmt1 mutation. Together, these data show that hepatocyte nuclear morphogenesis coincides with organ morphogenesis and outgrowth, and that DNA methylation directs chromatin organization, and, in turn, hepatocyte nuclear shape and size during liver development.
Comparison of the Transcriptomic and Epigenetic Profiles of Gonadal Primordial Germ Cells of White Leghorn and Green-Legged Partridgelike Chicken Embryos
Authors: Dunislawska Aleksandra , Siwek Maria , Stadnicka Katarzyna , Bednarczyk Marek
Published: July 2021

The Green-legged Partridgelike fowl is a native, dual-purpose Polish chicken. The White Leghorn has been intensively selected for several decades to mainly improve reproductive traits. Primordial germ cells (PGCs) represent the germline stem cells in chickens and are the only cells that can transfer the information stored in the genetic material from generation to generation. The aim of the study was to carry out a transcriptomic and an epigenetic comparison of the White Leghorn and Green-legged Partridgelike gonadal PGCs (gPGCs) at three developmental stages: days 4.5, 8, and 12 of the embryonic development. RNA and DNA were isolated from collected gPGCs. The RNA was further subjected to microarray analysis. An epigenetic analysis was performed based on the global methylation analysis and qMSP method for the particular silenced genes demonstrated in transcriptomic analysis. Statistically significant differences between the gPGCs from both breeds were detected on the day 8 of embryonic development. Global methylation analysis showed significant changes at the methylation level in the White Leghorn gPGCs on day 8 of embryonic development. The results suggest faster development of Green-legged Partridgelike embryos as compared to White Leghorn embryos. Changes in the levels of gene expression during embryonic development are determined by genetic and environmental factors, and this variability is influenced by breed and gender.
Assessment of Seismic Bedrock in Deep Alluvial Plains. Case Studies from the Emilia-Romagna Plain
Authors: Martelli Luca
Published: July 2021

The estimation of seismic shaking is essential for a realistic assessment of the local seismic hazard and the implementation of effective strategies for prevention and mitigation of the seismic risk. One of the most important aspects in the analysis of the site seismic assessment is the recognition of the seismic bedrock and its depth. Unfortunately, these data are not always easy to evaluate, especially in areas where the thickness of loose or poorly consolidated sediments is high. This article illustrates data and case studies from the Emilia-Romagna sector of the Po Plain, in order to provide examples and suggestions for the recognition of the seismic bedrock in alluvial and coastal areas characterised by significant thicknesses of unconsolidated sediments, using available data and not expensive geophysical surveys. The application of the proposed method indicates that the study area can be divided into four domains characterized by different depths of the seismic bedrock: the marginal or pede-Apennine belt, the high structural zones, the syncline/minor anticline zones, and the Po delta-coast zone.
Positional Accuracy Assessment of Digital Orthophoto Based on UAV Images: An Experience on an Archaeological Area
Authors: Korumaz Saadet Armağan Güleç , Yıldız Ferruh
Published: July 2021

Rapid development in UAV (unmanned aerial vehicle) photogrammetry made it preferable in many applications including cultural heritage documentation. Usability, quickness and accuracy of digital images have grabbed also the attention of archaeologists. Especially orthoimages by UAVs have become considerably significant in the field of archaeological heritage documentation since they are fast and accurate images of the object with high detailed information. However their accuracy and quality are the most important features of these images for archaeological documentation. The aim of this paper is to evaluate horizontal and vertical accuracy of an orthophoto taken by a fixed-wing UAV in an archaeological area. The evaluation is made according to ASPRS (American Society for Photogrammetry and Remote Sensing) Accuracy Standards for Digital Geospatial Data. The archaeological area, the name of which is Kubad Abad Palace in Beyşehir Province in Konya, is the only Anatolian Seljuk Palace structure that has survived to the present day. The study describes the orthophoto generation process and positional accuracy evaluation results within the frame of the importance of accuracy for archaeological documentation.
HBsAg Dampened STING Associated Activation of NK Cells in HBeAg-Negative CHB Patients
Authors: Zheng Bingqing , Yu Yating , Pan Zhaoyi , Feng Yujie , Zhao Huajun , Han Qiuju , Zhang Jian
Published: July 2021

NK cells play crucial roles in defending against persistent HBV. However, NK cells present dysfunction in chronic hepatitis B virus (CHB) infection, and the associated mechanism is still not fully understood. Except for the regulatory receptors, NK cells could also be regulated by the surface and intracellular pattern recognition receptors (PRRs). In the present study, we found that the level of the adaptor of DNA sensor STING in NK cells was significantly decreased in HBeAg-negative CHB patients, and it was positively associated with the degranulation ability of NK cells. Compared to NK cells from healthy donors, NK cells from HBeAg-negative CHB patients displayed a lower responsiveness to cGAMP stimulation. Further investigation showed that HBsAg could inhibit the STING expression in NK cells and suppress the response of NK cells to cGAMP. Significantly, STAT3 was identified to be a transcription factor that directly regulated STING transcription by binding to the promoter. In addition, STAT3 positively regulated the STING associated IFN-α response of NK cells. These findings suggested that STING is an important adaptor in NK cell recognition and activation, while HBsAg disturbs NK cell function by the STAT3-STING axis, providing a new mechanism of NK disability in HBeAg-negative CHB infection.
Cigarette Smoke Specifically Affects Small Airway Epithelial Cell Populations and Triggers the Expansion of Inflammatory and Squamous Differentiation Associated Basal Cells
Authors: Wohnhaas Christian T. , Gindele Julia A. , Kiechle Tobias , Shen Yang , Leparc Germán G. , Stierstorfer Birgit , Stahl Heiko , Gantner Florian , Viollet Coralie , Schymeinsky Jürgen , Baum Patrick
Published: July 2021

Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD) and causes remodeling of the small airways. However, the exact smoke-induced effects on the different types of small airway epithelial cells (SAECs) are poorly understood. Here, using air–liquid interface (ALI) cultures, single-cell RNA-sequencing reveals previously unrecognized transcriptional heterogeneity within the small airway epithelium and cell type-specific effects upon acute and chronic cigarette smoke exposure. Smoke triggers detoxification and inflammatory responses and aberrantly activates and alters basal cell differentiation. This results in an increase of inflammatory basal-to-secretory cell intermediates and, particularly after chronic smoke exposure, a massive expansion of a rare inflammatory and squamous metaplasia associated KRT6A+ basal cell state and an altered secretory cell landscape. ALI cultures originating from healthy non-smokers and COPD smokers show similar responses to cigarette smoke exposure, although an increased pro-inflammatory profile is conserved in the latter. Taken together, the in vitro models provide high-resolution insights into the smoke-induced remodeling of the small airways resembling the pathological processes in COPD airways. The data may also help to better understand other lung diseases including COVID-19, as the data reflect the smoke-dependent variable induction of SARS-CoV-2 entry factors across SAEC populations.
Integrative Transcriptomic and Proteomic Analysis Reveals an Alternative Molecular Network of Glutamine Synthetase 2 Corresponding to Nitrogen Deficiency in Rice (Oryza sativa L.)
Authors: Liang Ting , Yuan Zhengqing , Fu Lu , Zhu Menghan , Luo Xiaoyun , Xu Wuwu , Yuan Huanran , Zhu Renshan , Hu Zhongli , Wu Xianting
Published: July 2021

Nitrogen (N) is an essential nutrient for plant growth and development. The root system architecture is a highly regulated morphological system, which is sensitive to the availability of nutrients, such as N. Phenotypic characterization of roots from LY9348 (a rice variety with high nitrogen use efficiency (NUE)) treated with 0.725 mM NH4NO3 (1/4N) was remarkable, especially primary root (PR) elongation, which was the highest. A comprehensive analysis was performed for transcriptome and proteome profiling of LY9348 roots between 1/4N and 2.9 mM NH4NO3 (1N) treatments. The results indicated 3908 differential expression genes (DEGs; 2569 upregulated and 1339 downregulated) and 411 differential abundance proteins (DAPs; 192 upregulated and 219 downregulated). Among all DAPs in the proteome, glutamine synthetase (GS2), a chloroplastic ammonium assimilation protein, was the most upregulated protein identified. The unexpected concentration of GS2 from the shoot to the root in the 1/4N treatment indicated that the presence of an alternative pathway of N assimilation regulated by GS2 in LY9348 corresponded to the low N signal, which was supported by GS enzyme activity and glutamine/glutamate (Gln/Glu) contents analysis. In addition, N transporters (NRT2.1, NRT2.2, NRT2.3, NRT2.4, NAR2.1, AMT1.3, AMT1.2, and putative AMT3.3) and N assimilators (NR2, GS1;1, GS1;2, GS1;3, NADH-GOGAT2, and AS2) were significantly induced during the long-term N-deficiency response at the transcription level (14 days). Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that phenylpropanoid biosynthesis and glutathione metabolism were significantly modulated by N deficiency. Notably, many transcription factors and plant hormones were found to participate in root morphological adaptation. In conclusion, our study provides valuable information to further understand the response of rice roots to N-deficiency stress.
Genome Sequence of the Asian Honeybee in Pakistan Sheds Light on Its Phylogenetic Relationship with Other Honeybees
Authors: Tan Hongwei , Naeem Muhammad , Ali Hussain , Shakeel Muhammad , Kuang Haiou , Zhang Ze , Sun Cheng
Published: July 2021

In Pakistan, Apis cerana, the Asian honeybee, has been used for honey production and pollination services. However, its genomic makeup and phylogenetic relationship with those in other countries are still unknown. We collected A. cerana samples from the main cerana-keeping region in Pakistan and performed whole genome sequencing. A total of 28 Gb of Illumina shotgun reads were generated, which were used to assemble the genome. The obtained genome assembly had a total length of 214 Mb, with a GC content of 32.77%. The assembly had a scaffold N50 of 2.85 Mb and a BUSCO completeness score of 99%, suggesting a remarkably complete genome sequence for A. cerana in Pakistan. A MAKER pipeline was employed to annotate the genome sequence, and a total of 11,864 protein-coding genes were identified. Of them, 6750 genes were assigned at least one GO term, and 8813 genes were annotated with at least one protein domain. Genome-scale phylogeny analysis indicated an unexpectedly close relationship between A. cerana in Pakistan and those in China, suggesting a potential human introduction of the species between the two countries. Our results will facilitate the genetic improvement and conservation of A. cerana in Pakistan.
Kissing Bug Intrusions into Homes in the Southwest United States
Authors: Klotz Stephen A. , Smith Shannon L. , Schmidt Justin O.
Published: July 2021

Kissing bugs readily enter homes in the Sonoran Desert and bite the residents. Their saliva is highly antigenic, causing local and systemic skin reactions and life-threatening anaphylaxis. We attempted to determine what characteristics of homesites may have contributed to home intrusion by kissing bugs. Extensive and detailed information about the homes and the home environment was collected from 78 homeowners in Tucson who suffered kissing bug intrusions. Homeowners collected 298 Triatoma rubida in and around their homes. Of the homes entered by kissing bugs, 29 of 46 (63%) contained bugs harboring Trypanosoma cruzi. Although in the aggregate, homeowners were bitten > 2200 times, no individual tested positive for Chagas disease (N = 116). Although yearly intrusion likely occurs in some homes, T. rubida does not domiciliate within homesites in the Desert Southwest. We conclude there is little risk to homeowners for Chagas disease given the current behavior of resident kissing bugs and absent ingesting kissing bug fecal matter.
Increased Plasma Levels of Myosin Heavy Chain 11 Is Associated with Atherosclerosis
Authors: Takahashi Lisa , Ishigami Tomoaki , Tomiyama Hirofumi , Kato Yuko , Kikuchi Hiroyuki , Tasaki Koichiro , Yamashita Jun , Inoue Shigeru , Taguri Masataka , Nagao Toshitaka , Chikamori Taishiro , Ishikawa Yoshihiro , Yokoyama Utako
Published: July 2021

Many studies have revealed numerous potential biomarkers for atherosclerosis, but tissue-specific biomarkers are still needed. Recent lineage-tracing studies revealed that smooth muscle cells (SMCs) contribute substantially to plaque formation, and the loss of SMCs causes plaque vulnerability. We investigated the association of SMC-specific myosin heavy chain 11 (myosin-11) with atherosclerosis. Forty-five patients with atherosclerosis and 34 control subjects were recruited into our study. In the atherosclerosis patients, 35 patients had either coronary artery disease (CAD) or peripheral artery disease (PAD), and 10 had both CAD and PAD. Coronary arteries isolated from five patients were subjected to histological study. Circulating myosin-11 levels were higher in the CAD or PAD group than in controls. The area under the receiver operating characteristic curve of myosin-11 was 0.954. Circulating myosin-11 levels in the CAD and PAD group were higher than in the CAD or PAD group, while high-sensitivity C-reactive protein concentrations did not differ between these groups. Multinomial logistic regression analyses showed a significant association of myosin-11 levels with the presence of multiple atherosclerotic regions. Myosin-11 was expressed in the medial layer of human atherosclerotic lesions where apoptosis elevated. Circulating myosin-11 levels may be useful for detecting spatial expansion of atherosclerotic regions.
Cognitive Behavioral Therapy of Patients with Somatic Symptoms—Diagnostic and Therapeutic Difficulties
Authors: Orzechowska Agata , Maruszewska Paulina , Gałecki Piotr
Published: July 2021

In every somatic disease we can find a psychological element, just as it is not uncommon for numerous physical symptoms to occur in a mental disease. Nowadays, the patient is no longer just the “owner” of the sick organ but is considered and treated as a “whole”. The interpenetration of somatic manifestations with mental health problems forces patients who experience subjective suffering, including mental suffering, from current symptoms to visit specialists from different fields of medicine, and their treatment does not bring about any improvement. Cognitive behavioral psychotherapy (CBT) is one form of therapy that attempts to respond to the needs of an increasing—in recent years—number of patients who demonstrate somatic disorders of a multifaceted nature. The co-occurrence of physical and mental disorders repeatedly makes it impossible to determine which symptoms were the cause and which were the effect; hence, it is difficult to establish clear boundaries between the categories of these disorders and diseases. The therapist, to whom the patient with somatic diseases is eventually referred, may be faced with a diagnostic dilemma, the solution of which will give direction to further psychotherapeutic work. The common feature of this group of patients is a strong focus on physical ailments, while omitting or almost completely ignoring the psychological factors involved. The purpose of this paper is to present the causally diverse circumstances in which a patient with physical symptoms needs diagnosis and therapeutic support from the perspective of a cognitive behavioral approach.
Risk Factors for Non-Contact Lower-Limb Injury: A Retrospective Survey in Pediatric-Age Athletes
Authors: Guan Yanfei , Bredin Shannon S. D. , Taunton Jack , Jiang Qinxian , Wu Nana , Li Yongfeng , Warburton Darren E. R.
Published: July 2021

Background: Risk factors for non-contact lower-limb injury in pediatric-age athletes and the effects of lateral dominance in sport (laterally vs. non-laterally dominant sports) on injury have not been investigated. Purpose: To identify risk factors for non-contact lower-limb injury in pediatric-age athletes. Methods: Parents and/or legal guardians of 2269 athletes aged between 6–17 years were recruited. Each participant completed an online questionnaire that contained 10 questions about the athlete’s training and non-contact lower-limb injury in the preceding 12 months. Results: The multivariate logistic regression model determined that lateral dominance in sport (adjusted OR (laterally vs. non-laterally dominant sports), 1.38; 95% CI, 1.10–1.75; p = 0.006), leg preference (adjusted OR (right vs. left-leg preference), 0.71; 95% CI, 0.53–0.95; p = 0.023), increased age (adjusted OR, 1.21; 95% CI, 1.16–1.26; p = 0.000), training intensity (adjusted OR, 1.77; 95% CI, 1.43–2.19; p = 0.000), and training frequency (adjusted OR, 1.36; 95% CI, 1.25–1.48; p = 0.000) were significantly associated with non-contact lower-limb injury in pediatric-age athletes. Length of training (p = 0.396) and sex (p = 0.310) were not associated with a non-contact lower-limb injury. Conclusions: Specializing in laterally dominant sports, left-leg preference, increase in age, training intensity, and training frequency indicated an increased risk of non-contact lower-limb injury in pediatric-age athletes. Future research should take into account exposure time and previous injury.
The Incidence of Radial Artery Occlusion in Critically Ill Patients after Cannulation with a Long Catheter
Authors: Wujtewicz Magdalena , Regent Bartosz , Marszałek-Ratnicka Rozalia , Smugała Aneta , Szurowska Edyta , Owczuk Radosław
Published: July 2021

Cardiac output monitoring is a common practice in critically ill patients. The PiCCO (pulse index continuous cardiac output) method requires artery cannulation. According to the manufacturer, the cannula in the radial artery should be removed after three days. However, longer monitoring is sometimes necessary. The aim of this study was to assess the incidence of radial artery occlusion (RAO) after three days of cannulation and to check whether five-day cannulation is related to a higher occlusion rate. An additional assessment was made to verify the presence of occlusion three, fourteen and thirty days after decannulation. The PiCCO cannula was inserted into the radial artery after the Barbeau test and Doppler assessment of blood flow. It was left for three or five days. Doppler was performed immediately after its removal and at three, fourteen and thirty days following decannulation. Thirty-seven patients were randomly assigned for three or five days of cannulation, and twenty-three of them were eligible for further analysis. RAO was found in thirteen (56.5%) patients. No statistical difference was found between the RAO rate for three and five day cannulations (p = 0.402). The incidence of RAO was lower when the right radial artery was cannulated (p = 0.022; OR 0.129). Radial artery cannulation with a PiCCO catheter poses a risk of RAO. However, the incidence of prolonged cannulation appeared to not increase the risk of artery occlusion. ClinicalTrials.gov ID NCT02695407.
Effects of Recombinant Human Growth Hormone Treatment, Depending on the Therapy Start in Different Nutritional Phases in Paediatric Patients with Prader–Willi Syndrome: A Polish Multicentre Study
Authors: Lecka-Ambroziak Agnieszka , Wysocka-Mincewicz Marta , Doleżal-Ołtarzewska Katarzyna , Zygmunt-Górska Agata , Wędrychowicz Anna , Żak Teresa , Noczyńska Anna , Birkholz-Walerzak Dorota , Stawerska Renata , Hilczer Maciej , Obara-Moszyńska Monika , Rabska-Pietrzak Barbara , Gołębiowska Elżbieta , Dudek Adam , Petriczko Elżbieta , Szalecki Mieczysław ,   on behalf of the Polish Coordination Group for rhGH Treatment
Published: July 2021

Recombinant human growth hormone (rhGH) treatment is an established management in patients with Prader–Willi syndrome (PWS), with growth promotion and improvement in body composition and possibly the metabolic state. We compared anthropometric characteristics, insulin-like growth factor 1 (IGF1) levels, metabolic parameters and the bone age/chronological age index (BA/CA) in 147 children with PWS, divided according to age of rhGH start into four groups, corresponding to nutritional phases in PWS. We analysed four time points: baseline, rhGH1 (1.21 ± 0.81 years), rhGH2 (3.77 ± 2.17 years) and rhGH3 (6.50 ± 2.92 years). There were no major differences regarding height SDS between the groups, with a higher growth velocity (GV) (p = 0.00) and lower body mass index (BMI) SDS (p < 0.05) between the first and older groups during almost the whole follow-up. IGF1 SDS values were lower in group 1 vs. other groups at rhGH1 and vs. groups 2 and 3 at rhGH2 (p < 0.05). Glucose metabolism parameters were favourable in groups 1 and 2, and the lipid profile was comparable in all groups. BA/CA was similar between the older groups. rhGH therapy was most effective in the youngest patients, before the nutritional phase of increased appetite. We did not observe worsening of metabolic parameters or BA/CA advancement in older patients during a comparable time of rhGH therapy.
Comparison of Efficacy between 120° and 180° Schlemm’s Canal Incision Microhook Ab Interno Trabeculotomy
Authors: Okada Naoki , Hirooka Kazuyuki , Onoe Hiromitsu , Murakami Yumiko , Okumichi Hideaki , Kiuchi Yoshiaki
Published: July 2021

We compared surgical outcomes in patients with either primary open-angle glaucoma or exfoliation glaucoma after undergoing combined phacoemulsification with either a 120° or 180° incision during a Schlemm’s canal microhook ab interno trabeculotomy (μLOT-Phaco). This retrospective comparative case series examined 52 μLOT-Phaco eyes that underwent surgery between September 2017 and December 2020. Surgical qualified success was defined as an intraocular pressure (IOP) of ≤20 mmHg, ≥20% IOP reduction with IOP-lowering medications, and no additional glaucoma surgery. Success rates were evaluated by Kaplan-Meier survival analysis. The number of postoperative IOP-lowering medications and occurrence of complications were also assessed. Mean preoperative IOP in the 120° group was 16.9 ± 7.6 mmHg, which significantly decreased to 10.9 ± 2.7 mmHg (p < 0.01) and 11.1 ± 3.1 mmHg (p = 0.01) at 12 and 24 months, respectively. The mean number of preoperative IOP-lowering medications significantly decreased from 2.8 ± 1.4 to 1.4 ± 1.4 (p < 0.01) at 24 months. Mean preoperative IOP in the 180° group was 17.1 ± 7.0 mmHg, which significantly decreased to 12.1 ± 3.2 mmHg (p = 0.02) and 12.9 ± 1.4 mmHg (p = 0.01) at 12 and 24 months, respectively. The mean number of preoperative IOP-lowering medications significantly decreased from 2.9 ± 1.2 to 1.4 ± 1.5 (p < 0.01) at 24 months. The probability of qualified success at 24 months in the 120° and 180° groups was 50.4% and 54.6%, respectively (p = 0.58). There was no difference observed for hyphema formation or IOP spikes. Surgical outcomes were not significantly different between the 120° and 180° incisions in Schlemm’s canal.
Predictors of Response and Survival to Multikinase Inhibitors in Radioiodine Resistant Differentiated Thyroid Cancer
Authors: Feola Tiziana , Cozzolino Alessia , Centello Roberta , Pandozzi Carla , Tarsitano Maria Grazia , Giannetta Elisa
Published: July 2021

Sorafenib and lenvatinib are the only multikinase inhibitors (MKIs) approved for the treatment of radioactive iodine refractory differentiated thyroid cancer (RR-DTC). Although they have been demonstrated to improve progression free survival and overall response rate, the risk of toxicities is very high, worsening patients’ quality of life. Therefore, predicting MKI treatment outcomes in the setting of RR-DTC is very challenging for optimizing patients’ management. The current review provides an overview of the predictive factors for the response and survival of sorafenib and lenvatinib in RR-DTC. In this setting, a systemic therapy should be considered after conducting a multidisciplinary discussion aimed at evaluating the risk-benefit ratio of the treatment and taking into account several clinical, biochemical, and molecular factors. Age, performance status, and cancer-related symptoms are the most important clinical markers to be considered prior to starting MKI treatment, together with tumor burden. Some tissue and circulating biomarkers have been investigated, those involved in the angiogenic pathways being the most promising. Finally, prospective clinical trials aimed at evaluating predictive markers for therapeutic response are needed for tailoring patient management and allowing more appropriate treatment choices.
Association between CYP3A5 Polymorphism and Statin-Induced Adverse Events: A Systemic Review and Meta-Analysis
Authors: Yee Jeong , Kim Hamin , Heo Yunhee , Yoon Ha-Young , Song Gonjin , Gwak Hye-Sun
Published: July 2021

Purpose: Cytochrome P450 (CYP) is involved in the metabolism of statins; CYP3A5 is the main enzyme responsible for lipophilic statin metabolism. However, the evidence of the association between CYP3A5*3 polymorphism and the risk of statin-induced adverse events remains unclear. Therefore, this study aimed to perform a systematic review and meta-analysis to investigate the relationship between the CYP3A5*3 polymorphism and the risk of statin-induced adverse events. Methods: The PubMed, Web of Science, and EMBASE databases were searched for qualified studies published until August 2020. Observational studies that included the association between statin-induced adverse events and the CYP3A5*3 polymorphism were reviewed. The odds ratios (ORs) and 95% confidence intervals (CIs) were evaluated to assess the strength of the relationship. The Mantel–Haenszel method was used to provide the pooled ORs. Heterogeneity was estimated with I2 statistics and publication bias was determined by Begg’s and Egger’s test of the funnel plot. Data analysis was performed using Review Manager (version 5.4) and R Studio (version 3.6). Results: In total, data from 8 studies involving 1614 patients were included in this meta-analysis. The CYP3A5*3 polymorphism was found to be associated with the risk of statin-induced adverse events (*3/*3 vs. *1/*1 + *1/*3: OR = 1.40, 95% CI = 1.08–1.82). For myopathy, the pooled OR was 1.30 (95% CI: 0.96–1.75). The subgroup analysis of statin-induced myopathy revealed a trend, which did not achieve statistical significance. Conclusions: This meta-analysis demonstrated that the CYP3A5*3 polymorphism affected statin-induced adverse event risk. Therefore, CYP3A5 genotyping may be useful to predict statin toxicity.
A Novel Energy-Efficient Clustering Algorithm for More Sustainable Wireless Sensor Networks Enabled Smart Cities Applications
Authors: Yousif Zahid , Hussain Intesab , Djahel Soufiene , Hadjadj-Aoul Yassine
Published: July 2021

Wireless Sensor Networks (WSNs) is a major sensing technology that has revolutionized the way information is collected, processed, and used in many smart cities’ applications that rely on sensing technologies for event detection and monitoring. Despite the multiple benefits that such technology offers, the quick depletion of sensors’ battery power represents a major concern, mainly due to the extensive computational tasks and communication operations performed by individual sensors. Indeed, the cost of replacing batteries can be prohibitively expensive, especially when sensors are deployed in areas where access is difficult, in urbanized cities. To extend sensors’ lifetime, this paper proposes a new variant of LEACH protocol named LEACH enhanced with probabilistic cluster head selection (LEACH-PRO). LEACH-PRO introduces several measures to extend WSNs nodes’ lifetime such as cluster head node selection using a probabilistic function based on maximum residual energy and minimum distance to the sink. The obtained simulation results have proven the supremacy of LEACH-PRO over LEACH and direct transmission protocol in terms of the achieved network lifetime and the generated traffic overhead. Most importantly, LEACH-PRO will significantly extend the sensors’ lifetime, which would make this type of deployment more viable in smart city scenarios.
Sambaquis from the Southern Brazilian Coast: Landscape Building and Enduring Heterarchical Societies throughout the Holocene
Authors: DeBlasis Paulo , Gaspar Madu , Kneip Andreas
Published: July 2021

This paper presents a heterarchical model for the regional occupation of the sambaqui (shellmound) societies settled in the southern coast of Santa Catarina, Brazil. Interdisciplinary approaches articulate the geographical scope and environmental dynamics of the Quaternary with human occupation patterns that took place therein between the middle and late Holocene (approximately 7.5 to 1.5 ky BP). The longue durée perspective on natural and social processes, as well as landscape construction, evince stable, integrated, and territorially organized communities around the lagoon setting. Funerary patterns, as well as mound distribution in the landscape, indicate a rather equalitarian society, sharing the economic use of coastal resources in cooperative ways. This interpretation is reinforced by a common ideological background involving the cult of the ancestors, which seems widespread all over the southern Brazilian shores along that period of time. Such a long-lived cultural tradition has endured until the arrival of fully agricultural Je and Tupi speaking societies in the southern shores.