bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation of Antioxidant Protein Hydrolysates from Pleurotus geesteranus and Their Protective Effects on H2O2 Oxidative Damaged PC12 Cells
Authors: Liao Xiyu , Zhu Zhenjun , Wu Shujian , Chen Mengfei , Huang Rui , Wang Juan , Wu Qingping , Ding Yu
Published: November 2020

Pleurotus geesteranus is a promising source of bioactive compounds. However, knowledge of the antioxidant behaviors of P. geesteranus protein hydrolysates (PGPHs) is limited. In this study, PGPHs were prepared with papain, alcalase, flavourzyme, pepsin, and pancreatin, respectively. The antioxidant properties and cytoprotective effects against oxidative stress of PGPHs were investigated using different chemical assays and H2O2 damaged PC12 cells, respectively. The results showed that PGPHs exhibited superior antioxidant activity. Especially, hydrolysate generated by alcalase displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (91.62%), 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) radical scavenging activity (90.53%), ferric reducing antioxidant power, and metal ion-chelating activity (82.16%). Analysis of amino acid composition revealed that this hydrolysate was rich in hydrophobic, negatively charged, and aromatic amino acids, contributing to its superior antioxidant properties. Additionally, alcalase hydrolysate showed cytoprotective effects on H2O2-induced oxidative stress in PC12 cells via diminishing intracellular reactive oxygen species (ROS) accumulation by stimulating antioxidant enzyme activities. Taken together, alcalase hydrolysate of P. geesteranus protein can be used as beneficial ingredients with antioxidant properties and protective effects against ROS-mediated oxidative stress.
Binding Studies of AICAR and Human Serum Albumin by Spectroscopic, Theoretical, and Computational Methodologies
Authors: Hashempour Shokoufeh , Shahabadi Nahid , Adewoye Aishat , Murphy Brennen , Rouse Camaray , Salvatore Brian A. , Stratton Christopher , Mahdavian Elahe
Published: November 2020

The interactions of small molecule drugs with plasma serum albumin are important because of the influence of such interactions on the pharmacokinetics of these therapeutic agents. 5-Aminoimidazole-4-carboxamide ribonucleoside (AICAR) is one such drug candidate that has recently gained attention for its promising clinical applications as an anti-cancer agent. This study sheds light upon key aspects of AICAR’s pharmacokinetics, which are not well understood. We performed in-depth experimental and computational binding analyses of AICAR with human serum albumin (HSA) under simulated biochemical conditions, using ligand-dependent fluorescence sensitivity of HSA. This allowed us to characterize the strength and modes of binding, mechanism of fluorescence quenching, validation of FRET, and intermolecular interactions for the AICAR–HSA complexes. We determined that AICAR and HSA form two stable low-energy complexes, leading to conformational changes and quenching of protein fluorescence. Stern–Volmer analysis of the fluorescence data also revealed a collision-independent static mechanism for fluorescence quenching upon formation of the AICAR–HSA complex. Ligand-competitive displacement experiments, using known site-specific ligands for HSA’s binding sites (I, II, and III) suggest that AICAR is capable of binding to both HSA site I (warfarin binding site, subdomain IIA) and site II (flufenamic acid binding site, subdomain IIIA). Computational molecular docking experiments corroborated these site-competitive experiments, revealing key hydrogen bonding interactions involved in stabilization of both AICAR–HSA complexes, reaffirming that AICAR binds to both site I and site II.
The Effect of Filtration on Physical and Chemical Properties of Osmo-Dehydrated Material
Authors: Masztalerz Klaudia , Figiel Adam , Michalska-Ciechanowska Anna , Wojdyło Aneta , Nowicka Paulina , Lech Krzysztof
Published: November 2020

Osmotic dehydration (OD) performed in concentrated fruit juices used as osmotic solution (OS) comes with some limitations resulting from the material cell structure and is not entirely recognized at the moment. Filtration of the juice could provide some insight into the phenomena occurring throughout the OD. Therefore, the main aim of the study was to recognize the mechanism of selective penetration during OD and evaluate the effect of filtration on physical and chemical properties of osmo-dehydrated material. For this purpose, OD of pumpkin in non-filtrated and filtrated (filters 0.2, 0.45, 0.8, 1.2, 3, 5 and 8 μm) concentrated chokeberry juice was carried out in the study. Moreover, scanning electron microscope (SEM) images were provided. Total phenolic content (TPC) and antioxidant capacity measured by Ferric Reducing Antioxidant Potential (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC ABTS) of OS and the material were determined. It was found that even though filtration of osmotic solution had a moderate influence on the mass transfer, it greatly affected the chemical composition of dehydrated material. The best option, considering both chemical and physical properties of the dehydrated material, is the use of non-filtrated solution. However, when shorter time of OD is considered, much better results are obtained for filtrated solutions.
Structural Study of the Photo-Mediated Growth of Silver Nanoprisms
Authors: Knaapila Matti , Vainio Ulla , Canton Sophie E. , Karlsson Gunnel
Published: November 2020

We present a small-angle X-ray scattering (SAXS) study of the anisotropic photoinduced growth of silver (Ag) nanoprisms in aqueous dispersions. The growth of nearly spherical (<10 nm) Ag particles into large (>40 nm) and thin (<10 nm) triangular nanoprisms induced by 550 nm laser is followed in terms of particle size using indirect and direct methods for irradiation times up to 150 min. During the process, the surface-to-volume ratio of the particles decreased. The SAXS data of the initial solution fit well to the model of polydisperse spheres with pronounced average diameters around 7.4 nm and 10 nm. The data after 45 min irradiation fit well to the model containing approximately the same amount of the initial particles and the end product, the nanoprisms.
Medicinal Activities and Nanomedicine Delivery Strategies for Brucea javanica Oil and Its Molecular Components
Authors: Yoon Bo Kyeong , Lim Zheng Yi , Jeon Won-Yong , Cho Nam-Joon , Kim Jeong Hoon , Jackman Joshua A.
Published: November 2020

Brucea javanica oil (BJO) is widely used in traditional Chinese medicine to treat various types of cancer and inflammatory diseases. There is significant interest in understanding the medicinal activities of BJO and its molecular components, especially quassinoids, and in exploring how they can be incorporated into nanomedicine delivery strategies for improved application prospects. Herein, we cover the latest progress in developing different classes of drug delivery vehicles, including nanoemulsions, liposomes, nanostructured lipid carriers, and spongosomes, to encapsulate BJO and purified quassinoids. An introduction to the composition and medicinal activities of BJO and its molecular components, including quassinoids and fatty acids, is first provided. Application examples involving each type of drug delivery vehicle are then critically presented. Future opportunities for nanomedicine delivery strategies in the field are also discussed and considered within the context of translational medicine needs and drug development processes.
Ab-Initio Molecular Dynamics Simulation of Condensed-Phase Reactivity: The Electrolysis of Amino Acids and Peptides
Authors: Kiakojouri Ali , Nadimi Ebrahim , Frank Irmgard
Published: November 2020

Electrolysis is a potential candidate for a quick method of wastewater cleansing. However, it is necessary to know what compounds might be formed from bioorganic matter. We want to know if there are toxic intermediates and if it is possible to influence the product formation by the variation in initial conditions. In the present study, we use Car–Parrinello molecular dynamics to simulate the fastest reaction steps under such circumstances. We investigate the behavior of amino acids and peptides under anodic conditions. Such highly reactive situations lead to chemical reactions within picoseconds, and we can model the reaction mechanisms in full detail. The role of the electric current is to discharge charged species and, hence, to produce radicals from ions. This leads to ultra-fast radical reactions in a bulk environment, which can also be seen as redox reactions as the oxidation states change. In the case of amino acids, the educts can be zwitterionic, so we also observe complex acid–base chemistry. Hence, we obtain the full spectrum of condensed-phase chemistry.
Thuja occidentalis L. (Cupressaceae): Ethnobotany, Phytochemistry and Biological Activity
Authors: Caruntu Sonia , Ciceu Alina , Olah Neli Kinga , Don Ioan , Hermenean Anca , Cotoraci Coralia
Published: November 2020

Thuja occidentalis L. (Cupressaceae) has its origins in Eastern North America and is cultivated in Europe and Brazil as an ornamental tree, being known as the “tree of life” or “white cedar”. In traditional medicine, it is commonly used to treat liver diseases, bullous bronchitis, psoriasis, enuresis, amenorrhea, cystitis, uterine carcinomas, diarrhea, and rheumatism. The chemical constituents of T. occidentalis have been of research interest for decades, due to their contents of essential oil, coumarins, flavonoids, tannins, and proanthocyanidines. Pharmacology includes antioxidant, anti-inflammatory, antibacterial, antifungal, anticancer, antiviral, protective activity of the gastrointestinal tract, radioprotection, antipyretic, and lipid metabolism regulatory activity. Therefore, the present review represents the synthesis of all the relevant information for T. occidentalis, its ethnobotany, phytochemistry, and a thorough analysis of their pharmacological activities, in order to promote all the biological activities shown so far, rather than the antitumor activity that has promoted it as a medicinal species.
Developments in Vascular-Targeted Photodynamic Therapy for Urologic Malignancies
Authors: Nogueira Lucas , Tracey Andrew T. , Alvim Ricardo , Reisz Peter , Scherz Avigdor , Coleman Jonathan A. , Kim Kwanghee
Published: November 2020

With improved understanding of cancer biology and technical advancements in non-invasive management of urological malignancies, there is renewed interest in photodynamic therapy (PDT) as a means of focal cancer treatment. The application of PDT has also broadened as a result of development of better-tolerated and more effective photosensitizers. Vascular-targeted PDT (VTP) using padeliporfin, which is a water-soluble chlorophyll derivative, allows for tumor-specific cytotoxicity and has demonstrated efficacy in the management of urologic malignancies. Herein, we describe the evolution of photodynamic therapy in urologic oncology and the role of VTP in emerging treatment paradigms.
Rare-Earth Metal Complexes of the Antibacterial Drug Oxolinic Acid: Synthesis, Characterization, DNA/Protein Binding and Cytotoxicity Studies
Authors: Maciuca Ana-Madalina , Munteanu Alexandra-Cristina , Mihaila Mirela , Badea Mihaela , Olar Rodica , Nitulescu George Mihai , Munteanu Cristian V. A. , Bostan Marinela , Uivarosi Valentina
Published: November 2020

“Drug repositioning” is a current trend which proved useful in the search for new applications for existing, failed, no longer in use or abandoned drugs, particularly when addressing issues such as bacterial or cancer cells resistance to current therapeutic approaches. In this context, six new complexes of the first-generation quinolone oxolinic acid with rare-earth metal cations (Y3+, La3+, Sm3+, Eu3+, Gd3+, Tb3+) have been synthesized and characterized. The experimental data suggest that the quinolone acts as a bidentate ligand, binding to the metal ion via the keto and carboxylate oxygen atoms; these findings are supported by DFT (density functional theory) calculations for the Sm3+ complex. The cytotoxic activity of the complexes, as well as the ligand, has been studied on MDA-MB 231 (human breast adenocarcinoma), LoVo (human colon adenocarcinoma) and HUVEC (normal human umbilical vein endothelial cells) cell lines. UV-Vis spectroscopy and competitive binding studies show that the complexes display binding affinities (Kb) towards double stranded DNA in the range of 9.33 × 104 − 10.72 × 105. Major and minor groove-binding most likely play a significant role in the interactions of the complexes with DNA. Moreover, the complexes bind human serum albumin more avidly than apo-transferrin.
Obtaining Granules from Waste Tannery Shavings and Mineral Additives by Wet Pulp Granulation
Authors: Ławińska Katarzyna , Szufa Szymon , Modrzewski Remigiusz , Obraniak Andrzej , Wężyk Tomasz , Rostocki Andrzej , Olejnik Tomasz P.
Published: November 2020

This paper presents the results of research on the granulation process of leather industry waste, i.e., tanning shavings. It is economically justified to granulate this waste together with mineral additives that are useful in the processes of their further processing. Unfortunately, the granulation of raw, unsorted shavings does not obtain desired results due to their unusual properties. In this study, the possibilities of agglomeration of this waste were examined by a new method consisting of the production and then the granulation of wet pulp. During granulation, no additional binding liquid is added to the granulated bed. As part of this work, the specific surface of granulated shavings, the granulometric composition of the obtained agglomerates, and their strength parameters were determined. The use of a vibrating disc granulator, the addition of a water glass solution (in the pulp), dolomite, and gypsum made it possible to obtain durable, mechanically stable granules.
Redox Potential and Antioxidant Capacity of Bovine Bone Collagen Peptides towards Stable Free Radicals, and Bovine Meat Lipids and Proteins. Effect of Animal Age, Bone Anatomy and Proteases—A Step Forward towards Collagen-Rich Tissue Valorisation
Authors: Aubry Laurent , De-Oliveira-Ferreira Claude , Santé-Lhoutellier Véronique , Ferraro Vincenza
Published: November 2020

Collagen antioxidant peptides are being widely studied. However, no research has paid attention to biological parameters such as the age and anatomy of collagen-rich tissues, which can determine a change in tissue structure and composition, and then in bioactivity. Moreover, only few research works have studied and assessed peptides antioxidant activity on the food matrix. This work aimed to investigate the effect of bovine’s bone age and anatomy, and of six different enzymes, on the antioxidant activity of collagen peptides. Collagen was extracted from young and old bovine femur and tibia; six different enzymes were used for peptides’ release. The redox potential, the quenching of stable free radicals, and the antioxidant capacity on bovine meat lipids and proteins was evaluated, under heating from ambient temperature to 80 °C. Age and anatomy showed a significant effect; the influence of anatomy becomes most important with age. Each enzyme’s effectiveness toward age and anatomy was not the same. The greatest amount of peptides was released from young bones’ collagen hydrolysed with papain. The antioxidant activity was higher at higher temperatures, except for meat proteins. Assessing the effect of age and anatomy of collagen-rich tissues can promote a better application of collagen bioactive peptides.
Optical Diagnostics of Supercritical CO2 and CO2-Ethanol Mixture in the Widom Delta
Authors: Mareev Evgenii , Semenov Timur , Lazarev Alexander , Minaev Nikita , Sviridov Alexander , Potemkin Fedor , Gordienko Vyacheslav
Published: November 2020

The supercritical CO2 (scCO2) is widely used as solvent and transport media in different technologies. The technological aspects of scCO2 fluid applications strongly depend on spatial–temporal fluctuations of its thermodynamic parameters. The region of these parameters’ maximal fluctuations on the p-T (pressure-temperature) diagram is called Widom delta. It has significant practical and fundamental interest. We offer an approach that combines optical measurements and molecular dynamics simulation in a wide range of pressures and temperatures. We studied the microstructure of supercritical CO2 fluid and its binary mixture with ethanol in a wide range of temperatures and pressures using molecular dynamics (MD) simulation. MD is used to retrieve a set of optical characteristics such as Raman spectra, refractive indexes and molecular refraction and was verified by appropriate experimental measurements. We demonstrated that in the Widom delta the monotonic dependence of the optical properties on the CO2 density is violated. It is caused by the rapid increase of density fluctuations and medium-sized (20–30 molecules) cluster formation. We identified the correlation between cluster parameters and optical properties of the media; in particular, it is established that the clusters in the Widom delta acts as a seed for clustering in molecular jets. MD demonstrates that the cluster formation is stronger in the supercritical CO2-ethanol mixture, where the extended binary clusters are formed; that is, the nonlinear refractive index significantly increased. The influence of the supercritical state in the cell on the formation of supersonic cluster jets is studied using the Mie scattering technique.
Targeting Beta-Blocker Drug–Drug Interactions with Fibrinogen Blood Plasma Protein: A Computational and Experimental Study
Authors: González-Durruthy Michael , Concu Riccardo , Vendrame Laura F. Osmari , Zanella Ivana , Ruso Juan M. , Cordeiro M. Natália D. S.
Published: November 2020

In this work, one of the most prevalent polypharmacology drug–drug interaction events that occurs between two widely used beta-blocker drugs—i.e., acebutolol and propranolol—with the most abundant blood plasma fibrinogen protein was evaluated. Towards that end, molecular docking and Density Functional Theory (DFT) calculations were used as complementary tools. A fibrinogen crystallographic validation for the three best ranked binding-sites shows 100% of conformationally favored residues with total absence of restricted flexibility. From those three sites, results on both the binding-site druggability and ligand transport analysis-based free energy trajectories pointed out the most preferred biophysical environment site for drug–drug interactions. Furthermore, the total affinity for the stabilization of the drug–drug complexes was mostly influenced by steric energy contributions, based mainly on multiple hydrophobic contacts with critical residues (THR22: P and SER50: Q) in such best-ranked site. Additionally, the DFT calculations revealed that the beta-blocker drug–drug complexes have a spontaneous thermodynamic stabilization following the same affinity order obtained in the docking simulations, without covalent-bond formation between both interacting beta-blockers in the best-ranked site. Lastly, experimental ultrasound density and velocity measurements were performed and allowed us to validate and corroborate the computational obtained results.
Characterization of Phenolic Compounds, Vitamin E and Fatty Acids from Monovarietal Virgin Olive Oils of “Picholine marocaine” Cultivar
Authors: Bouymajane Aziz , Oulad El Majdoub Yassine , Cacciola Francesco , Russo Marina , Salafia Fabio , Trozzi Alessandra , Rhazi Filali Fouzia , Dugo Paola , Mondello Luigi
Published: November 2020

Olive oil is an important product in the Mediterranean diet, due to its health benefits and sensorial characteristics. Picholine marocaine is the most cultivated variety in Morocco. The present research aims to evaluate the phenolic compounds, vitamin E and fatty acids of commercial Picholine marocaine virgin olive oils (VOOs) from five different North Moroccan provinces (Chefchaouen, Taounate, Errachidia, Beni Mellal and Taza), using HPLC-photodiode array (PDA)/electrospray ionization (ESI)-MS, normal phase (NP)-HPLC/ fluorescence detector (FLD) and GC-flame ionization detector (FID)/MS, respectively. The obtained results showed an average content of 130.0 mg kg−1 of secoiridoids (oleuropein aglycone, 10-hydroxy-oleuropein aglycone and ligstroside aglycone, oleocanthal and oleacein), 108.1 mg kg−1 of phenolic alcohols (tyrosol and hydroxytyrosol), 34.7 mg kg−1 of phenolic acids (caffeic acid, ferulic acid and elenolic acid), and 8.24 mg kg−1 of flavonoids (luteolin, luteolin glucoside, apigenin). With regard to vitamin E, α-tocopherol was the most abundant vitamin E (57.9 mg kg−1), followed by α-tocotrienol (2.5 mg kg−1), γ-tocopherol (4.5 mg kg−1) and β-tocopherol (1.9 mg kg−1), while δ-tocopherol was not detected. Moreover, 14 fatty acids were found and, among them, oleic acid (76.1%), linoleic acid (8.1%) palmitic acid (8.7%) and stearic acid (2.5%) were the major fatty acids detected. Finally, heat map and principal component analysis allowed us to classify the studied provinces in terms of VOO chemical composition: Chefchaouen (tyrosol and hydroxytyrosol), Taounate (oleuropein aglycone), Errachidia (ferulic acid, w-3 and w-6), Beni Mellal (oleocanthal) and Taza (luteolin and oleic acid).
Effects of β-glucan Rich Barley Flour on Glucose and Lipid Metabolism in the Ileum, Liver, and Adipose Tissues of High-Fat Diet Induced-Obesity Model Male Mice Analyzed by DNA Microarray
Authors: Mio Kento , Yamanaka Chiemi , Matsuoka Tsubasa , Kobayashi Toshiki , Aoe Seiichiro
Published: November 2020

We evaluated whether intake of β-glucan-rich barley flour affects expression levels of genes related to glucose and lipid metabolism in the ileum, liver, and adipose tissues of mice fed a high-fat diet. C57BL/6J male mice were fed a high-fat diet supplemented with high β-glucan barley, for 92 days. We measured the expression levels of genes involved in glucose and lipid metabolism in the ileum, liver, and adipose tissues using DNA microarray and q-PCR. The concentration of short-chain fatty acids (SCFAs) in the cecum was analyzed by GC/MS. The metabolic syndrome indices were improved by barley flour intake. Microarray analysis showed that the expression of genes related to steroid synthesis was consistently decreased in the liver and adipose tissues. The expression of genes involved in glucose metabolism did not change in these organs. In liver, a negative correlation was showed between some SCFAs and the expression levels of mRNA related to lipid synthesis and degradation. Barley flour affects lipid metabolism at the gene expression levels in both liver and adipose tissues. We suggest that SCFAs are associated with changes in the expression levels of genes related to lipid metabolism in the liver and adipose tissues, which affect lipid accumulation.
Zinc Deficiency—An Independent Risk Factor in the Pathogenesis of Haemorrhagic Stroke?
Authors: Grüngreiff Kurt , Gottstein Thomas , Reinhold Dirk
Published: November 2020

Zinc is an essential trace element for human health and plays a fundamental role in metabolic, immunological and many other biological processes. The effects of zinc are based on the intra- and extracellular regulatory function of the zinc ion (Zn2+) and its interactions with proteins. The regulation of cellular zinc homeostasis takes place via a complex network of metal transporters and buffering systems that react to changes in the availability of zinc in nutrition, chronic diseases, infections and many other processes. Zinc deficiency is associated with impairment of numerous metabolic processes, reduced resistance to infections due to impaired immune functions, changes in skin and its appendages and disorders of wound healing and haemostasis. While ischemic heart attacks (myocardial infarction) occur more frequently with meat-based normal diets, haemorrhagic strokes are more frequently observed with vegetarian/vegan diets. The causes are discussed as deficiencies of various micronutrients, such as vitamin B12, vitamin D, various amino acids and also zinc. In the present review, after a description of the functions of zinc and its resorption, a discussion of daily food intake will follow, with a special focus on the importance of food composition and preparation for the zinc balance. The close interrelationships between proteins, especially albumin and zinc will be discussed. Finally, the possible causes and consequences of a zinc deficiency on the blood vessels and blood coagulation are considered.
Remyelination-Promoting DNA Aptamer Conjugate Myaptavin-3064 Binds to Adult Oligodendrocytes In Vitro
Authors: Fereidan-Esfahani Mahboubeh , Yue Wei Ying , Wilbanks Brandon , Johnson Aaron J. , Warrington Arthur E. , Howe Charles L. , Rodriguez Moses , Maher Louis J.
Published: November 2020

We previously applied Systematic Evolution of Ligands by EXponential enrichment (SELEX) technology to identify myelin-specific DNA aptamers, using crude mouse central nervous system myelin as bait. This selection identified a 40-nucleotide aptamer (LJM-3064). Multiple biotinylated LJM-3064 molecules were conjugated to a streptavidin core to mimic a multimeric immunoglobulin M (IgM) antibody, generating 3064-BS-streptavidin (Myaptavin-3064). We previously showed that Myaptavin-3064 induces remyelination in the Theiler’s murine encephalomyelitis virus (TMEV) model of chronic spinal cord demyelination. While details of target binding and the mechanism of action remain unclear, we hypothesized that Myaptavin-3064 induces remyelination by binding to oligodendrocytes (OLs). We now report the results of binding assays using the human oligodendroglioma (HOG) cell line, applying both flow cytometry and immunocytochemistry (IC) to assay aptamer conjugate binding to cells. IC assays were applied to compare aptamer conjugate binding to primary embryonic mouse mixed cortical cultures and primary adult rat mixed glial cultures. We show that Myaptavin-3064 binds to HOG cells, with increased binding upon differentiation. In contrast, a negative control aptamer conjugate, 3060-BS, which did not promote central nervous system (CNS) remyelination, does not bind to HOG cells. Myaptavin-3064 did not bind to lung (L2) or kidney (BHK) cell lines. Total internal reflection fluorescence (TIRF) imaging indicates that Myaptavin-3064 binds at the cell membrane of live cells. In addition to HOG cells, Myaptavin-3064 binds to adult rat OLs, but not to embryonic mouse mixed cortical cultures. These data support the hypothesis that Myaptavin-3064 binds to a surface molecule on both rodent and human OLs in a manner that triggers a remyelination signal pathway.
Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae
Authors: El-Garawani Islam , El-Seedi Hesham , Khalifa Shaden , El Azab Islam H. , Abouhendia Marwa , Mahmoud Shaymaa
Published: November 2020

The usage of insects as a sustainable and functional natural products resource is a new promise in complementary and alternative medicine. The present study aimed to investigate the ability of Musca domestica (housefly) larval hemolymph (insect blood) to display the enhanced in vitro antioxidant and cytotoxic effects. The oxidative stress (OS) was elicited by inducing lipopolysaccharides (LPS) treatment as an exogenous stressor. Determination of superoxide dismutase 1 (SOD1), glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC), and mRNA and protein expressions of SOD1, was investigated as confirmatory markers of oxidative stress induction. Cytotoxicity on cancerous MCF-7 and normal Vero cells were also evaluated using an MTT assay at 24 h post-injection. The injection of LPS induced a significant (p < 0.05) increase in SOD, GSH and TAC, whereas, the MDA was diminished. Hemolymph was collected from normal and treated larvae after 6, 12 and 24 h. The M. domestica superoxide dismutase (MdSOD1) transcripts were significantly (p < 0.05) upregulated 6 and 12 h post-treatment, while a significant downregulation was observed after 24 h. Western blot analysis showed that MdSOD1 was expressed in the hemolymph of the treated larvae with an increase of 1.2 folds at 6 and 12 h and 1.6 folds at 24 h relative to the control group. LPS-treated larval hemolymphs exhibited significant cytotoxicity with respect to the untreated ones against MCF-7 while Vero cells showed no cytotoxicity for both hemolymphs. The DPPH free radical scavenging activity was examined and a significant antioxidant potential potency was observed at 6 h (50% maximal inhibitory concentration (IC50): 63.3 ± 3.51 µg/mL) when compared to the control M. domestica larval hemolymph (IC50: 611.7 ± 10.41 µg/mL). Taken together, M. domestica larval hemolymph exhibited enhanced antioxidant and consequently increased cytotoxic capacities under stressed conditions.
Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior in Simulated Human Body Fluids and Effect on Macrophages
Authors: Keil Claudia , Hübner Christopher , Richter Constanze , Lier Sandy , Barthel Lars , Meyer Vera , Subrahmanyam Raman , Gurikov Pavel , Smirnova Irina , Haase Hajo
Published: November 2020

Chronic non-healing wounds represent a substantial economic burden to healthcare systems and cause a considerable reduction in quality of life for those affected. Approximately 0.5–2% of the population in developed countries are projected to experience a chronic wound in their lifetime, necessitating further developments in the area of wound care materials. The use of aerogels for wound healing applications has increased due to their high exudate absorbency and ability to incorporate therapeutic substances, amongst them trace metals, to promote wound-healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate aerogels and their metal release upon incubation in human sweat or wound fluid substitutes. All aerogels show excellent liquid uptake from any of the formulas and high liquid holding capacities. Calcium is only marginally released into the swelling solvents, thus remaining as alginate bridging component aiding the absorption and fast transfer of liquids into the aerogel network. The zinc transfer quota is similar to those observed for common wound dressings in human and animal injury models. With respect to the immune regulatory function of zinc, cell culture studies show a high availability and anti-inflammatory activity of aerogel released Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial effectiveness versus cytotoxicity remains a significant challenge for which the alginate aerogels need to be improved in the future. An increased knowledge of the transformations that alginate aerogels undergo in the course of the fabrication as well as during wound fluid exposure is necessary when aiming to create advanced, tissue-compatible aerogel products.
Kinetic Study of the Thermal and Thermo-Oxidative Degradations of Polystyrene Reinforced with Multiple-Cages POSS
Authors: Blanco Ignazio , Cicala Gianluca , Tosto Claudio , Bottino Francesco Agatino
Published: November 2020

A comprehensive kinetics degradation study is carried out on novel multiple cages polyhedral oligomeric silsesquioxane (POSS)/polystyrene (PS) composites at 5% (w/w) of POSS to assess their thermal behavior with respect to the control PS and other similar POSS/PS systems studied in the past. The composites are synthesized by in situ polymerization of styrene in the presence of POSSs and characterized by 1H-NMR. The characteristics of thermal parameters are determined using kinetics literature methods, such as those developed by Kissinger and Flynn, Wall, and Ozawa (FWO), and discussed and compared with each other and with those obtained in the past for similar POSS/PS composites. A good improvement in the thermal stability with respect to neat polymer is found, but not with respect to those obtained in the past for polystyrene reinforced with single- or double-POSS cages. This behavior is attributed to the greater steric hindrance of the three-cages POSS compared with those of single- or double-cage POSS molecules.
Production of Fuels and Chemicals from a CO2/H2 Mixture
Authors: Yao Yali , Sempuga Baraka Celestin , Liu Xinying , Hildebrandt Diane
Published: November 2020

In order to explore co-production alternatives, a once-through process for CO2 hydrogenation to chemicals and liquid fuels was investigated experimentally. In this approach, two different catalysts were considered; the first was a Cu-based catalyst that hydrogenates CO2 to methanol and CO and the second a Fisher–Tropsch (FT) Co-based catalyst. The two catalysts were loaded into different reactors and were initially operated separately. The experimental results show that: (1) the Cu catalyst was very active in both the methanol synthesis and reverse-water gas shift (R-WGS) reactions and these two reactions were restricted by thermodynamic equilibrium; this was also supported by an Aspen plus simulation of an (equilibrium) Gibbs reactor. The Aspen simulation results also indicated that the reactor can be operated adiabatically under certain conditions, given that the methanol reaction is exothermic and R-WGS is endothermic. (2) the FT catalyst produced mainly CH4 and short chain saturated hydrocarbons when the feed was CO2/H2. When the two reactors were coupled in series and the presence of CO in the tail gas from the first reactor (loaded with Cu catalyst) significantly improves the FT product selectivity toward higher carbon hydrocarbons in the second reactor compared to the standalone FT reactor with only CO2/H2 in the feed.
Community Mental Health Nursing Consultation in a Public Bathhouse: A Spiritual Coping Resource
Authors: Simões Figueiredo Amélia , Rasquilho Vidal Teresa , Ferrito Cândida , Neves-Amado João , Sarreira-Santos Alexandra , Medeiros-Garcia Lurdes , Roldán-Merino Juan , Vollrath Ramirez Antonia
Published: November 2020

(1) Background: The spiritual dimension’s importance in health/disease processes is widely recognized, also being demonstrated by scientific evidence. Hence, its study is crucial, particularly with respect to a Mental health nursing consultation occurring in a community resource, such as a public bathhouse. This study aims to identify the nursing interventions of spiritual nature developed over 5 years in the abovementioned setting, thus characterizing the Portuguese reality; (2) Methods: Observational, cross-sectional and quantitative study, based on a sample comprising all users who attended a community Mental health nursing consultation, from March 2015, to 31 December 2019; (3) Results: A total of 205 nursing appointments were performed, from which emerged a set of 346 diagnoses, resulting in 455 nursing interventions. Of the latter, some deserve to be highlighted, due to a greater prevalence: “listening” (61; 13%), “supporting” (38; 8%), “promoting self-esteem” (37; 8%), “monitoring vital signs” (31; 7%), and “identifying attitude towards care” (25; 5%); (4) Conclusions: even though some of the interventions performed during the consultations were associated with the spiritual dimension, the collected data points towards a need for new diagnoses and nursing interventions, namely, those which may help mitigate spiritual distress.
A Decade of Modern Bridge Monitoring Using Terrestrial Laser Scanning: Review and Future Directions
Authors: Rashidi Maria , Mohammadi Masoud , Sadeghlou Kivi Saba , Abdolvand Mohammad Mehdi , Truong-Hong Linh , Samali Bijan
Published: November 2020

Over the last decade, particular interest in using state-of-the-art emerging technologies for inspection, assessment, and management of civil infrastructures has remarkably increased. Advanced technologies, such as laser scanners, have become a suitable alternative for labor intensive, expensive, and unsafe traditional inspection and maintenance methods, which encourage the increasing use of this technology in construction industry, especially in bridges. This paper aims to provide a thorough mixed scientometric and state-of-the-art review on the application of terrestrial laser scanners (TLS) in bridge engineering and explore investigations and recommendations of researchers in this area. Following the review, more than 1500 research publications were collected, investigated and analyzed through a two-fold literature search published within the last decade from 2010 to 2020. Research trends, consisting of dominated sub-fields, co-occurrence of keywords, network of researchers and their institutions, along with the interaction of research networks, were quantitatively analyzed. Moreover, based on the collected papers, application of TLS in bridge engineering and asset management was reviewed according to four categories including (1) generation of 3D model, (2) quality inspection, (3) structural assessment, and (4) bridge information modeling (BrIM). Finally, the paper identifies the current research gaps, future directions obtained from the quantitative analysis, and in-depth discussions of the collected papers in this area.
Uncertainty Analysis of Object-Based Land-Cover Classification Using Sentinel-2 Time-Series Data
Authors: Ma Lei , Schmitt Michael , Zhu Xiaoxiang
Published: November 2020

Recently, time-series from optical satellite data have been frequently used in object-based land-cover classification. This poses a significant challenge to object-based image analysis (OBIA) owing to the presence of complex spatio-temporal information in the time-series data. This study evaluates object-based land-cover classification in the northern suburbs of Munich using time-series from optical Sentinel data. Using a random forest classifier as the backbone, experiments were designed to analyze the impact of the segmentation scale, features (including spectral and temporal features), categories, frequency, and acquisition timing of optical satellite images. Based on our analyses, the following findings are reported: (1) Optical Sentinel images acquired over four seasons can make a significant contribution to the classification of agricultural areas, even though this contribution varies between spectral bands for the same period. (2) The use of time-series data alleviates the issue of identifying the “optimal” segmentation scale. The finding of this study can provide a more comprehensive understanding of the effects of classification uncertainty on object-based dense multi-temporal image classification.
Laser-Facilitated Additive Manufacturing Enables Fabrication of Biocompatible Neural Devices
Authors: Behrens Ailke , Stieghorst Jan , Doll Theodor , Froriep Ulrich P.
Published: November 2020

Current personalized treatment of neurological diseases is limited by availability of appropriate manufacturing methods suitable for long term sensors for neural electrical activities in the brain. An additive manufacturing process for polymer-based biocompatible neural sensors for chronic application towards individualized implants is here presented. To process thermal crosslinking polymers, the developed extrusion process enables, in combination with an infrared (IR)-Laser, accelerated curing directly after passing the outlet of the nozzle. As a result, no additional curing steps are necessary during the build-up. Furthermore, the minimal structure size can be achieved using the laser and, in combination with the extrusion parameters, provide structural resolutions desired. Active implant components fabricated using biocompatible materials for both conductive pathways and insulating cladding keep their biocompatible properties even after the additive manufacturing process. In addition, first characterization of the electric properties in terms of impedance towards application in neural tissues are shown. The printing toolkit developed enables processing of low-viscous, flexible polymeric thermal curing materials for fabrication of individualized neural implants.
Cross-Modal Effect of Presenting Food Images on Taste Appetite
Authors: Tomono Keisuke , Tomono Akira
Published: November 2020

We researched a method to objectively evaluate the presence of food images, for the purpose of applying it to digital signage. In this paper, we defined the presence of food images as a sensation that makes us recognize that food is there, and investigated the relationship between that recognition and the salivary secretion reaction. If saliva secretion can be detected by a non-invasive method, it may be possible to objectively estimate the presence of the viewer from the outside. Two kinds of experiments were conducted. STUDY 1 included presentations of popular cooking images, which portrayed a sense of deliciousness, and evaluated changes in the volume of saliva secretions and cerebral blood flow near the temples. STUDY 2 included comparisons of changes between presenting images only and images with corresponded smells. The images included scenes that introduced foods (i.e., almond pudding cake/bergamot orange) that were relatively simple, so that they did not induce the subjects themselves. As a result, we clarified the cross-modal effects that were closely related to sense of presence and salivation. Moreover, we clarified presentation of images with smells to improve one’s sense of presence, even though the images were relatively simple.
An Innovative Approach to Surveying the Geometry of Visibility Triangles at Railway Level Crossings
Authors: Kampczyk Arkadiusz
Published: November 2020

Railway level crossings (RLCs) in Poland are classified according to their protection systems. Category D, which is a form of passive RLC, aims to ensure safe and efficient operation. Surveying is essential to prepare and control the geometry of the visibility triangles used at RLCs. This article presents a new approach to monitoring the geometry of visibility triangles of RLCs using an electronic total station and a magnetic measuring square (MMS). Its main assumptions are presented together with the application of the innovative measuring instruments. Visibility is demonstrated taking into account the angles of intersection of the road axis with the track axis of the railway line and additional attributes related to the analysis and evaluation of general visibility conditions. The research highlights controversies that have received special attention against the background of the safety status of railway level crossings. As a case study, the RLC located on a single-track railway line in Poland is examined. The final section presents applications of the results obtained according to the proposed methodology. It is shown that the proposed approach is practical and effective. In addition to surveyors, the survey methodology can be used by road and rail traffic engineers and policy makers to further improve traffic safety at RLCs. This is an important global research task.
A Stackelberg Game-Based Caching Incentive Scheme for Roadside Units in VANETs
Authors: Wang Yang , Lin Yuankun , Chen Lingyu , Shi Jianghong
Published: November 2020

As a key technology of intelligent transportation systems (ITS), vehicular ad hoc networks (VANETs) have been promising to provide safety and infotainment for drivers and passengers. To support different applications about traffic safety, traffic efficiency, autonomous driving and entertainment, it is important to investigate how to effectively deliver content in VANETs. Since it takes resources such as bandwidth and power for base stations (BSs) or roadside units (RSUs) to deliver content, the optimal pricing strategy for BSs and the optimal caching incentive scheme for RSUs need to be studied. In this paper, a framework of content delivery is proposed first, where each moving vehicle can obtain small-volume content files from either the nearest BS or the nearest RSU according to the competition among them. Then, the profit models for both BSs and RSUs are established based on stochastic geometry and point processes theory. Next, a caching incentive scheme for RSUs based on Stackelberg game is proposed, where both competition sides (i.e., BSs and RSUs) can maximize their own profits. Besides, a backward introduction method is introduced to solve the Stackelberg equilibrium. Finally, the simulation results demonstrate that BSs can obtain their own optimal pricing strategy for maximizing the profit as well as RSUs can obtain the optimal caching scheme with the maximum profit during the content delivery.
Effects of Wearable Devices with Biofeedback on Biomechanical Performance of Running—A Systematic Review
Authors: Giraldo-Pedroza Alexandra , Lee Winson Chiu-Chun , Lam Wing-Kai , Coman Robyn , Alici Gursel
Published: November 2020

This present review includes a systematic search for peer-reviewed articles published between March 2009 and March 2020 that evaluated the effects of wearable devices with biofeedback on the biomechanics of running. The included articles did not focus on physiological and metabolic metrics. Articles with patients, animals, orthoses, exoskeletons and virtual reality were not included. Following the PRISMA guidelines, 417 articles were first identified, and nineteen were selected following the removal of duplicates and articles which did not meet the inclusion criteria. Most reviewed articles reported a significant reduction in positive peak acceleration, which was found to be related to tibial stress fractures in running. Some previous studies provided biofeedback aiming to increase stride frequencies. They produced some positive effects on running, as they reduced vertical load in knee and ankle joints and vertical displacement of the body and increased knee flexion. Some other parameters, including contact ground time and speed, were fed back by wearable devices for running. Such devices reduced running time and increased swing phase time. This article reviews challenges in this area and suggests future studies can evaluate the long-term effects in running biomechanics produced by wearable devices with biofeedback.
Ligand-Based and Structured-Based In Silico Repurposing Approaches to Predict Inhibitors of SARS-CoV-2 Mpro Protein
Authors: Juárez-Saldívar Alfredo , Lara-Ramírez Edgar E. , Reyes-Espinosa Francisco , Paz-González Alma D. , Villalobos-Rocha Juan Carlos , Rivera Gildardo
Published: November 2020

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a coronavirus that causes the pandemic Coronavirus Disease 2019 (COVID-19). There is no current specific treatment for this new coronavirus. In this study, we employed a virtual screening repurposing strategy to search for potential SARS-CoV-2 Mpro inhibitors. The databases PDB, ChEMBL, BindingDB and DrugBank were queried with several filtering steps based on ligand-based and structure-based approaches. As a result, we obtained 58 molecules (37 from ChEMBL and 21 from DrugBank) that potentially inhibit SARS-CoV-2 Mpro. These molecules have on their chemical structure functional groups that favor stronger docking scores than the inhibitor N3. Several of these molecules are reported experimentally as SARS-CoV Mpro inhibitors. Hence, a combined virtual screening strategy allowed finding chemical compounds with a high potential for the inhibition of SARS-CoV-2 Mpro.