bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Germination on Fatty Acid Composition in Cereal Grains
Authors: Al-Taher Fadwa , Nemzer Boris
Published: September 2023

Sprouted grains are gaining popularity as functional food ingredients. This study aimed to evaluate the lipid and fatty acid composition of eight sprouted grains (millet, amaranth, quinoa, wheat, rye, barley, buckwheat, and oat). The method used was germination for up to 72 h at temperatures ranging from 19–23 °C. In general, the lipid content increased in the various grains sprouted, providing a rich source of polyunsaturated fatty acids. The % oil yield ranged from 1.17 ± 0.02% in sprouted rye to 5.71 ± 0.26% in sprouted amaranth. Germinated oat showed the greatest increase in fat content, 54.3%, compared to the control. Polyunsaturated fatty acids were more prevalent in whole grains (46.9–75.6%) than saturated fatty acids (10.1–25.9%) and increased with sprouting. The primary fatty acids detected in the grains, in order of abundance, were linoleic, oleic, palmitic, linolenic, and stearic acids. Millet sprouts contained the lowest total saturated fatty acids and the highest polyunsaturated fatty acids. Amaranth had the highest amount of saturated fatty acids, while buckwheat contained the lowest quantity of polyunsaturated fatty acids. The lowest omega-6/omega-3 ratio was 7 to 1 in sprouted rye and 8 to 1 in sprouted barley.
The Principle of Steam Explosion Technology and Its Application in Food Processing By-Products
Authors: Wang Changrong , Lin Mengfan , Yang Qingyu , Fu Chenying , Guo Zebin
Published: September 2023

Steam explosion technology is an emerging pretreatment method that has shown great promise for food processing due to its ability to efficiently destroy the natural barrier structure of materials. This narrative review summarizes the principle of steam explosion technology, its similarities and differences with traditional screw extrusion technology, and the factors that affect the technology. In addition, we reviewed the applications in food processing by-products in recent years. The results of the current study indicate that moderate steam explosion treatment can improve the quality and extraction rate of the target products. Finally, we provided an outlook on the development of steam explosion technology with a reference for a wider application of this technology in the food processing field.
Heme Oxygenase 1-Mediated Anti-Inflammatory Effect of Extract from the Aerial Part of Heracleum moellendorffii Hance
Authors: Jang Hyun Young , Lee Syng-Ook
Published: September 2023

In this study, the anti-inflammatory effects of a methanolic extract from the aerial part of Heracleum moellendorffii Hance (HmAPE) and its underlying mechanisms were investigated. HmAPE demonstrated a significant reduction in nitric oxide production in lipopolysaccharide (LPS)-treated murine macrophage RAW264.7 cells, and HmAPE decreased the protein and mRNA expression of inducible nitric oxide synthase. Further mechanistic studies on inflammatory signaling pathways revealed that HmAPE-mediated downregulation of inflammatory gene expressions was not associated with mitogen-activated protein kinases or nuclear factor-κB signaling pathways. However, HmAPE treatment activated nuclear factor E2-related factor 2 (Nrf2) and upregulated heme oxygenase-1 (HO-1) expression, which is known to suppress pro-inflammatory cytokine production. Additionally, treatment with a selective HO-1 inhibitor, tin protoporphyrin IX, partially reversed the effects of HmAPE in LPS-treated RAW264.7 cells, indicating that HmAPE inhibited LPS-induced NO production, at least in part, through induction of Nrf2-mediated HO-1 expression. These findings suggest that HmAPE could serve as a potential edible source with anti-inflammatory properties, and further studies are required to ascertain its anti-inflammatory efficacy in vivo.
Exploring the Impact of Human–Animal Connections and Trust in Labeling Consumers’ Intentions to Buy Cage-Free Eggs: Findings from Taiwan
Authors: Chang Min-Yen , Chao Ching-Tzu , Chen Han-Shen
Published: September 2023

Recently, interest in animal welfare has steadily increased, driven by a growing focus on environmental, social, and governance (ESG) issues. This trend aligns with the Sustainable Development Goals (SDGs) set by the United Nations. This underscores the importance of comprehending consumer attitudes towards products that are respectful to animal welfare. This research aims to employ the Value-Attitude-Behavior (VAB) model as a theoretical basis to explore the behavioral intentions of Taiwanese consumers to buy cage-free eggs. To provide comprehensive insights into consumer behavior in this domain, the study examines key variables such as ‘human–nature connectedness’, ‘trust in labels’, and ‘attitude towards animal welfare’. By addressing an existing research gap in the literature and exploring consumer behavior in relation to animal welfare-friendly products, the research contributes to an area that has received limited attention. We gathered a total of 341 valid questionnaires for this research. Employing Structural Equation Modeling (SEM) along with Maximum Likelihood Estimation (MLE), we investigated the causal connections between variables. The study presents three key observations: First, consumers who value sustainability and ethics tend to maintain a positive perspective on free-range eggs. Second, a favorable stance towards cage-free eggs directly correlates with positive future behaviors. Lastly, label trustworthiness and a supportive view towards animal welfare heavily affect behavioral intentions in consumers. Given these insights and the growing significance of animal welfare in consumer choices, we recommend that participants in the food and livestock sector earnestly advocate for and back practices that prioritize animal well-being.
Chestnut Shell Polyphenols Inhibit the Growth of Three Food-Spoilage Bacteria by Regulating Key Enzymes of Metabolism
Authors: Wang Xinfang , Li Yue , Liu Suwen , Wang Hao , Chang Xuedong , Zhang Jingzheng
Published: September 2023

The microbial contamination of food poses a threat to human health. Chestnut shells, which are byproducts of chestnut processing, contain polyphenols that exert various physiological effects, and thus have the potential to be used in food preservation. This study investigates the bacteriostatic effect and mechanism(s) of the action of chestnut shell polyphenols (CSPs) on three food-spoilage bacteria, namely Bacillus subtilis, Pseudomonas fragi, and Escherichia coli. To this end, the effect of CSPs on the ultrastructure of each bacterium was determined using scanning electron microscopy and transmission electron microscopy. Moreover, gene expression was analyzed using RT-qPCR. Subsequent molecular docking analysis was employed to elucidate the mechanism of action employed by CSPs via the inhibition of key enzymes. Ultrastructure analysis showed that CSPs damaged the bacterial cell wall and increased permeability. At 0.313 mg/mL, CSPs significantly increased the activity of alkaline phosphatase and lactate dehydrogenase, as well as protein leakage (p < 0.05), whereas the activity of the tricarboxylic acid (TCA) cycle enzymes, isocitrate dehydrogenase and α-ketoglutarate dehydrogenase, were inhibited (p < 0.05). The expression levels of the TCA-related genes gltA, icd, sucA, atpA, citA, odhA, IS178_RS16090, and IS178_RS16290 are also significantly downregulated by CSP treatment (p < 0.05). Moreover, CSPs inhibit respiration and energy metabolism, including ATPase activity and adenosine triphosphate (ATP) synthesis (p < 0.05). Molecular docking determined that proanthocyanidins B1 and C1, the main components of CSPs, are responsible for the antibacterial activity. Therefore, as natural antibacterial substances, CSPs have considerable potential for development and application as natural food preservatives.
Fast and Simple UPLC–Q-TOF MS Method for Determination of Bitter Flavan-3-ols and Oligomeric Proanthocyanidins: Impact of Vegetable Protein Fining Agents on Red Wine Composition
Authors: Dias Lara , Milheiro Juliana , Ribeiro Miguel , Fernandes Cristina , Neves Nuno , Filipe-Ribeiro Luís , Cosme Fernanda , Nunes Fernando M.
Published: September 2023

Wine phenolic compounds, particularly proanthocyanidins (PAs), play a significant role in wine sensory characteristics, specifically bitterness and astringency. Although not consensual, flavan-3-ols and oligomeric PAs are generally considered the primary contributors to wine bitterness. Patatin, a vegetable protein fining agent, has been explored as an alternative to animal and synthetic fining agents for reducing wine bitterness. However, contradictory results exist regarding its effectiveness in removing flavan-3-ols and oligomeric PAs in red wines. In this work, a UPLC–Q-TOF MS/MS method was optimized and validated for accurately measuring flavan-3-ols, as well as dimeric and trimeric PAs, in red wines. The MS/MS analysis of flavan-3-ols, in addition to the typical fragmentation described in the literature, revealed an intense mass fragment resulting from the loss of C3O2 and C3O2 + H2O from the parent ion. It was observed that flavan-3-ols and PAs undergo oxidation during sample preparation, which was reversed by the addition of 5 g/L of ascorbic acid. The method demonstrated good linearity range (2 mg/L to 20 mg/L), detection limit (0.3 mg/L to 0.7 mg/L), quantification limit (0.8 mg/L to 2.2 mg/L), precision (repeatability 2.2% to 7.3%), and accuracy (recovery 98.5% to 100.5%). The application of patatin at different doses (5 g/L to 30 g/L) in two different red wine matrices did not reduce the levels of monomeric, dimeric, and trimeric PAs in red wines. However, similar behaviors were observed for pea protein and gelatin. Therefore, wine fining trials and efficiency measurements of the treatments in each matrix are strongly advised.
Dandelion (Taraxacum mongolicum) Extract Alleviated H2O2-Induced Oxidative Damage: The Underlying Mechanism Revealed by Metabolomics and Lipidomics
Authors: Chen Yannan , Fei Siyuan , Yu Xiaoting , Tan Mingqian
Published: September 2023

Dandelion has received wide attention in food and medicine fields due to its excellent antioxidant properties. Nonetheless, the underlying mechanism of this action has not yet been fully clarified, particularly at the metabolic level. Herein, the effects of dandelion extract (DE) on H2O2-induced oxidative damage was investigated. The results indicate that the DE alleviated H2O2-induced cell damage (increased by 14.5% compared to H2O2 group), reduced the reactive oxygen species (ROS) level (decreased by 80.1% compared to H2O2 group), maintained the mitochondrial membrane potential (MMP) level, and increased antioxidant-related enzyme activities. Importantly, the metabolic response of PC12 cells indicates that H2O2 disturbed phospholipid metabolism and damaged cell membrane integrity. In addition, energy metabolism, the central nervous system, and the antioxidant-related metabolism pathway were perturbed. In contrast, DE rescued the H2O2-induced metabolic disorder and further alleviated oxidative damage. Collectively, these findings provide valuable stepping stones for a discussion of the mechanism and show the promise of DE as a suitable additive for functional food products.
Effects of Fermentation on Bioactivity and the Composition of Polyphenols Contained in Polyphenol-Rich Foods: A Review
Authors: Yang Fan , Chen Chao , Ni Derang , Yang Yubo , Tian Jinhu , Li Yuanyi , Chen Shiguo , Ye Xingqian , Wang Li
Published: September 2023

Polyphenols, as common components with various functional activities in plants, have become a research hotspot. However, researchers have found that the bioavailability and bioactivity of plant polyphenols is generally low because they are usually in the form of tannins, anthocyanins and glycosides. Polyphenol-rich fermented foods (PFFs) are reported to have better bioavailability and bioactivity than polyphenol-rich foods, because polyphenols are used as substrates during food fermentation and are hydrolyzed into smaller phenolic compounds (such as quercetin, kaempferol, gallic acid, ellagic acid, etc.) with higher bioactivity and bioavailability by polyphenol-associated enzymes (PAEs, e.g., tannases, esterases, phenolic acid decarboxylases and glycosidases). Biotransformation pathways of different polyphenols by PAEs secreted by different microorganisms are different. Meanwhile, polyphenols could also promote the growth of beneficial bacteria during the fermentation process while inhibiting the growth of pathogenic bacteria. Therefore, during the fermentation of PFFs, there must be an interactive relationship between polyphenols and microorganisms. The present study is an integration and analysis of the interaction mechanism between PFFs and microorganisms and is systematically elaborated. The present study will provide some new insights to explore the bioavailability and bioactivity of polyphenol-rich foods and greater exploitation of the availability of functional components (such as polyphenols) in plant-derived foods.
Proximate Composition and Bioactive Compounds of Cocoa Bean Shells as a By-Product from Cocoa Industries in Indonesia
Authors: Djali Mohamad , Santasa Kimberly , Indiarto Rossi , Subroto Edy , Fetriyuna Fetriyuna , Lembong Elazmanawati
Published: September 2023

Cocoa bean shell (CBS) is a by-product from cocoa processing which is abundant in Indonesia, one of the largest cocoa-producing countries. It has a great potential for being processed into food ingredients due to its comparable composition to cocoa nibs. The present study was conducted to identify the proximate composition and bioactive compounds in CBS produced at several cocoa industries in Indonesia utilizing different cocoa varieties (Criollo and Forastero) and processing techniques (fermented, non-fermented, pulp washing, and drying), which remain unknown. The results showed that the CBS derived from roasted Criollo cocoa pods in the Kendeng Lembu cocoa industry had ash and protein content of about 8.21% and 18.79%, respectively, which was higher than other industries. Additionally, the concentration of bioactive substances was higher here than it was elsewhere. This included total phenolic (136.2 mg GAE g−1) and theobromine (22.50 mg g−1). The lowest ash and protein concentration found in CBS was from Forastero cocoa pods, non-fermented like Sulawesi cocoa. These values were 6.48% and 15.70%, respectively. The concentration of theobromine (15.40 mg g−1) was also lower compared to other industries.
A Multi-Enzyme Cascade Response for the Colorimetric Recognition of Organophosphorus Pesticides Utilizing Core-Shell Pd@Pt Nanoparticles with High Peroxidase-like Activity
Authors: Majid Zainabu , Zhang Qi , Yang Zhansen , Che Huilian , Cheng Nan
Published: September 2023

In modern agricultural practices, organophosphorus pesticides or insecticides (OPs) are regularly used to restrain pests. Their limits are closely monitored since their residual hinders the capability of acetylcholinesterase (AChE) and brings out a threatening accumulation of the neurotransmitter acetylcholine (ACh), which affects human well-being. Therefore, spotting OPs in food and the environment is compulsory to prevent human health. Several techniques are available to identify OPs but encounter shortcomings like time-consuming, operating costs, and slow results achievement, which calls for further solutions. Herein, we present a rapid colorimetric sensor for quantifying OPs in foods using TMB as a substrate, a multi-enzyme cascade system, and the synergistic property of core-shell Palladinum@Platinum (Pd@Pt) nanoparticles. The multi-enzyme cascade response framework is a straightforward and effective strategy for OPs recognition and can resolve the previously mentioned concerns. Numerous OPs, including Carbofuran, Malathion, Parathion, Phoxim, Rojor, and Phosmet, were successfully quantified at different concentrations. The cascade method established using Pd@Pt had a simple and easy operation, a lower detection limit range of (1–2.5 ng/mL), and a short detection time of about 50 min. With an R2 value of over 0.93, OPs showed a linear range of 10–200 ng/mL, portraying its achievement in quantifying pesticide residue. Lastly, the approach was utilized in food samples and recovered more than 80% of the residual OPs.
Chenopodium quinoa’s Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet
Authors: Garcia Tejedor Aurora , Haros Claudia Monika , Laparra Llopis José Moisés
Published: September 2023

This study explored the effects of Chenopodium quinoa’s ingredients on the major lipids’ hepatic profile and the functional selective differentiation of monocyte-derived macrophages and innate lymphoid cells in mice on a high-fat diet. Six-week-old Rag2-/- and Rag2-/-Il2-/- mice received (12 days) a low-molecular-weight protein fraction (LWPF) or the lipid fraction (qLF) obtained from the cold pressing of C. quinoa’s germen. At the end of the experiment, mouse serum and liver tissue were collected. The differences in triglycerides, phospholipids, and the major lipids profile were analyzed. Infiltrated monocyte-derived macrophages and innate lymphoid cells (ILCs) and the expression of liver metabolic stress-related mRNA were measured. In the Rag2-/- mice, feeding them LWPF appeared to improve, to a larger extent, their hepatic capacity to utilize fatty acids in comparison to the qLF by preventing the overwhelming of triglycerides (TGs), despite both reducing the hepatic lipid accumulation. An analysis of the hepatic major lipids profile revealed significant increased variations in the PUFAs and phospholipid composition in the Rag2-/- mice fed with the LWPF or LF. The Rag2-/-Il2-/- mice, lacking innate and adaptive lymphocytes, seemed resistant to mobilizing hepatic TGs and unresponsive to lipid accumulation when fed with the LF. Notably, only the Rag2-/- mice fed with the LWPF showed an increased proportion of hepatic CD68+F4/80+ cells population, with a better controlled expression of the innate immune ‘Toll-like’ receptor (TLR)-4. These changes were associated with an oriented expansion of pluripotential CD117+ cells towards ILC2s (CD117+KLRG1+). Thus, C. quinoa’s ingredients resulted in being advantageous for improving the mechanisms for controlling the hepatic lipotoxicity derived from a high-fat diet, promoting liver macrophage and ILCs expansion to a selective functional differentiation for the control of HFD-driven immune and metabolic disturbances.
Ethanolic Extract from Seed Residues of Sea Buckthorn (Hippophae rhamnoides L.) Ameliorates Oxidative Stress Damage and Prevents Apoptosis in Murine Cell and Aging Animal Models
Authors: Hua Zhongjie , Zhang Jiachan , Cheng Wenjing , Wang Changtao , Zhao Dan
Published: September 2023

Hippophae rhamnoides L. has been widely used in research and application for almost two decades. While significant progress was achieved in the examination of its fruits and seeds, the exploration and utilization of its by-products have received relatively less attention. This study aims to address this research gap by investigating the effects and underlying mechanisms of sea buckthorn seed residues both in vitro and in vivo. The primary objective of this study is to assess the potential of the hydroalcoholic extract from sea buckthorn seed residues (HYD-SBSR) to prevent cell apoptosis and mitigate oxidative stress damage. To achieve this, an H2O2-induced B16F10 cell model and a D-galactose-induced mouse model were used. The H2O2-induced oxidative stress model using B16F10 cells was utilized to evaluate the cellular protective and reparative effects of HYD-SBSR. The results demonstrated the cytoprotective effects of HYD-SBSR, as evidenced by reduced apoptosis rates and enhanced resistance to oxidative stress alongside moderate cell repair properties. Furthermore, this study investigated the impact of HYD-SBSR on antioxidant enzymes and peroxides in mice to elucidate its reparative potential in vivo. The findings revealed that HYD-SBSR exhibited remarkable antioxidant performance, particularly at low concentrations, significantly enhancing antioxidant capacity under oxidative stress conditions. To delve into the mechanisms underlying HYD-SBSR, a comprehensive proteomics analysis was conducted to identify differentially expressed proteins (DEPs). Additionally, a Gene Ontology (GO) analysis and an Encyclopedia of Genes and Genomes (KEGG) pathway cluster analysis were performed to elucidate the functional roles of these DEPs. The outcomes highlighted crucial mechanistic pathways associated with HYD-SBSR, including the PPAR signaling pathway, fat digestion and absorption, glycerophospholipid metabolism, and cholesterol metabolism. The research findings indicated that HYD-SBSR, as a health food supplement, exhibits favorable effects by promoting healthy lipid metabolism, contributing to the sustainable and environmentally friendly production of sea buckthorn and paving the way for future investigations and applications in the field of nutraceutical and pharmaceutical research.
Editorial to Special Issue—Food Brewing Technology and Brewing Microorganisms
Authors: Chen Yanru , Fu Guiming , Wang Jinjing , Cai Wenqin
Published: September 2023

Food brewing technology is an important technology in the modern worldwide food industry, which uses the specific traits of microorganisms to produce food by traditional or modern engineering techniques [...]
Recent Trends in Air Transport Research: A Bibliometric Analysis
Authors: Sallan Jose M. , Lordan Oriol
Published: September 2023

The aim of this manuscript is to detect recent trends in air transport research through a bibliometric analysis. We retrieved all articles published between 2013 and 2022 in the Q1 of the Transportation ranking of the Social Sciences Citation Index published in the Journal Citations Reports (Q1-T), and in the Journal of Air Transport Management. Among these records, we retained for the analysis the articles related to air transport. Analyzing the author keywords of articles and retaining the Top 10 cited articles in each of the samples, we identified six research topics for each of the samples. Both samples share the topics of Industry Analysis, Air Traffic Management and COVID-19 and Air Transport. The specific topics of Q1-T sample are High Speed Rail and Air Transport, Environmental Impact of Air Transport and UAV and Urban Air Mobility. We also found that materials of Q1-T are mainly written from the regulator perspective, and JATM materials from the perspective of airport and airline managers. We believe that these results are useful for administrators evaluating air transport academics, and air transport academics themselves looking for new avenues of research and stakeholders of the air transport industry interested in evidence-based decision making.
Development of Thixotropic Molecular Oleogels Comprising Alkylanilide Gelators by Using a Mixing Strategy
Authors: Ohsedo Yutaka
Published: September 2023

Molecular oleogels have the potential to be used as materials in healthcare applications. However, their design and synthesis are complex, thus requiring simple and effective methods for their preparation. This paper reports on alkylanilides that are low molecular-weight organogelators, which when appropriately mixed with different alkyl chain lengths could result in the formation of mixed molecular gels that exhibit excellent gel-forming ability and mechanical properties. In addition, the single and mixed molecular organogel systems were found to be applicable as single and mixed molecular oleogel systems capable of gelling oils such as olive oil and squalane. This has been found to be true, especially in molecular oleogel systems consisting of squalane, which is used as solvents in healthcare. The mixed squalene-molecular oleogel systems showed an increase in the critical (minimum) gelation concentration from 1.0 to 0.1 wt.% in the single system and an improvement in the thixotropic behavior recovery time. The thixotropic behavior of the molecular oleogels in the mixed system was quantitatively evaluated through dynamic viscoelasticity measurements; however, it was not observed for the single-system molecular oleogels. Scanning electron microscopy of the xerogels suggested that this behavior is related to the qualitative improvement of the network owing to the refinement of the mesh structure. These mixed molecular oleogels, composed of alkylanilides displaying such thixotropic behavior, could be used as candidates for ointment-base materials in the healthcare field.
Novel HSPG2 Gene Mutation Causing Schwartz–Jampel Syndrome in a Moroccan Family: A Literature Review
Authors: Brugnoni Raffaella , Marelli Daria , Iacomino Nicola , Canioni Eleonora , Cappelletti Cristina , Maggi Lorenzo , Ardissone Anna
Published: September 2023

Schwartz–Jampel syndrome type 1 (SJS1) is a rare autosomal recessive musculoskeletal disorder caused by various mutations in the HSPG2 gene encoding the protein perlecan, a major component of basement membranes. We report a novel splice mutation HSPG2(NM_005529.7):c.3888 + 1G > A and a known point mutation HSPG2(NM_005529.7):c.8464G > A, leading to the skipping of exon 31 and 64 in mRNA, respectively, in a Moroccan child with clinical features suggestive of SJS1 and carrying two compound heterozygous mutations in the HSPG2 gene detected by next-generation sequencing. Both parents harboured one mutation. Real-time and immunostaining analysis revealed down-regulation of the HSPG2 gene and a mild reduction in the protein in the muscle, respectively. We reviewed all genetically characterized SJS1 cases reported in literature, confirming the clinical hallmarks and unspecific instrumental data in our case. The genotype–phenotype correlation is very challenging in SJS1. Therapy is mainly focused on symptom management and several drugs have been administered with different efficacy.Here, we report the second case with spontaneous improvement.
Outcome Measures and Biomarkers for Clinical Trials in Hereditary Spastic Paraplegia: A Scoping Review
Authors: Siow Sue-Faye , Yeow Dennis , Rudaks Laura I. , Jia Fangzhi , Wali Gautam , Sue Carolyn M. , Kumar Kishore R.
Published: September 2023

Hereditary spastic paraplegia (HSP) is characterized by progressive lower limb spasticity. There is no disease-modifying treatment currently available. Therefore, standardized, validated outcome measures to facilitate clinical trials are urgently needed. We performed a scoping review of outcome measures and biomarkers for HSP to provide recommendations for future studies and identify areas for further research. We searched Embase, Medline, Scopus, Web of Science, and the Central Cochrane database. Seventy studies met the inclusion criteria, and eighty-three outcome measures were identified. The Spastic Paraplegia Rating Scale (SPRS) was the most widely used (27 studies), followed by the modified Ashworth Scale (18 studies) and magnetic resonance imaging (17 studies). Patient-reported outcome measures (PROMs) were infrequently used to assess treatment outcomes (28% of interventional studies). Diffusion tensor imaging, gait analysis and neurofilament light chain levels were the most promising biomarkers in terms of being able to differentiate patients from controls and correlate with clinical disease severity. Overall, we found variability and inconsistencies in use of outcome measures with a paucity of longitudinal data. We highlight the need for (1) a standardized set of core outcome measures, (2) validation of existing biomarkers, and (3) inclusion of PROMs in HSP clinical trials.
Genetic Diversity and Population Structure in Ethiopian Mustard (Brassica carinata A. Braun) as Revealed by Single Nucleotide Polymorphism Markers
Authors: Tesfaye Misteru , Feyissa Tileye , Hailesilassie Teklehaimanot , Kanagarajan Selvaraju , Zhu Li-Hua
Published: September 2023

Ethiopian mustard (Brassica carinata A. Braun) is currently one of the potential oilseeds dedicated to the production for biofuel and other bio-industrial applications. The crop is assumed to be native to Ethiopia where a number of diversified B. carinata germplasms are found and conserved ex situ. However, there is very limited information on the genetic diversity and population structure of the species. This study aimed to investigate the genetic diversity and population structure of B. carinata genotypes of different origins using high-throughput single nucleotide polymorphism (SNP) markers. We used Brassica 90K Illumina InfiniumTM SNP array for genotyping 90 B. carinata genotypes, and a total of 11,499 informative SNP markers were used for investigating the population structure and genetic diversity. The structure analysis, principal coordinate analysis (PcoA) and neighbor-joining tree analysis clustered the 90 B. carinata genotypes into two distinct subpopulations (Pop1 and Pop2). The majority of accessions (65%) were clustered in Pop1, mainly obtained from Oromia and South West Ethiopian People (SWEP) regions. Pop2 constituted dominantly of breeding lines and varieties, implying target selection contributed to the formation of distinct populations. Analysis of molecular variance (AMOVA) revealed a higher genetic variation (93%) within populations than between populations (7%), with low genetic differentiation (PhiPT = 0.07) and poor correlation between genetic and geographical distance (R = 0.02). This implies the presence of gene flow (Nm > 1) and weak geographical structure of accessions. Genetic diversity indices showed the presence of moderate genetic diversity in B. carinata populations with an average genetic diversity value (HE = 0.31) and polymorphism information content (PIC = 0.26). The findings of this study provide important and relevant information for future breeding and conservation efforts of B. carinata.
Coping with Fungal Diseases in Crops: New Advances in Genomics, Breeding and Management
Authors: Mazzucotelli Elisabetta , Mastrangelo Anna Maria
Published: September 2023

This Special Issue comprises a collection of eight peer-reviewed articles centered around the plant–pathogen interaction with the aim of proposing strategies that enhance plant resistance to pathogens and limit the damage to crop production, utilizing a multidisciplinary approach [...]
Editorial for the Special Issue “Genetics Studies on Wheat”
Authors: Ma Hongxiang
Published: September 2023

Wheat (Triticum aestivum L [...]
Multisite Lifestyle for Older People after the Fukushima Nuclear Disaster
Authors: Ito Naomi , Amir Isamu , Saito Hiroaki , Moriyama Nobuaki , Furuyama Ayako , Singh Priya , Montesino Stephanie , Yamamoto Chika , Sato Mika , Abe Toshiki , Zhao Tianchen , Tsubokura Masaharu
Published: September 2023

After the Fukushima nuclear power plant disaster in 2011, the Japanese government implemented a return policy, lifting most evacuation orders in former evacuation areas. Consequently, the return of residents is currently underway. However, it has become common for a large number of residents to carry out multisite living, a lifestyle involving returning to their hometown while maintaining their house at the evacuation site, or living at more than two sites. This report focuses on one aspect of the secondary effects of the nuclear incident, which forced affected residents to adopt a multisite lifestyle. Disasters always have a strong impact, via displacement, on those who are socially vulnerable, such as older people in an ageing society. They need intense support to resume their daily life as it was before the incident. For this report, we interviewed an elderly lady in her 90s, who is executing “multisite living” at evacuation sites, in order to obtain reassurance from neighbours and the local community. Our findings may provide valuable suggestions on how older people can restart their lives with the local community in an ageing society after disasters, which could apply to any kind of disaster preparedness.
Pragmatic Systemic Solutions to the Wicked and Persistent Problem of the Unprofessional Disruptive Physician in the Health System
Authors: Peisah Carmelle , Williams Betsy , Hockey Peter , Lees Peter , Wright Danette , Rosenstein Alan
Published: September 2023

We have always had and will always have “disruptive” or “dysfunctional” doctors behaving unprofessionally within healthcare institutions. Disruptive physician behaviour (also called “unprofessional behaviour”) was described almost 150 years ago, but remains a persistent, wicked problem in healthcare, largely fuelled by systemic inaction. In this Commentary, we aim to explore the following aspects from a systemic lens: (i) the gaps in understanding systemic resistance and difficulty in addressing this issue; and (ii) pragmatic approaches to its management in the healthcare system. In doing so, we hope to shift the systemic effect from nihilism and despair, to one of hopeful realism about disruptive or unprofessional behaviour. We suggest that solutions lie in cultural change to ensure systemic awareness, responsiveness and early intervention, and an understanding of what systemic failure looks like in this context. Staff education, policies and procedures that outline a consistent reporting and review process including triaging the problem, its source, its effects, and the attempted solutions, are also crucial. Finally, assessment and intervention from appropriately mental-health-trained personnel are required, recognising that this is a complex mental health problem. We are not doing anyone any favours by ignoring, acting as bystanders, or otherwise turning a blind eye to disruptive or unprofessional behaviour; otherwise, we share culpability.
Risk Factors Linked to Violence in Female Same-Sex Couples in Hispanic America: A Scoping Review
Authors: Garay-Villarroel Leonor , Castrechini-Trotta Angela , Armadans-Tremolosa Immaculada
Published: September 2023

Intimate partner violence (IPV) among women is an understudied topic in Hispanic Americans; therefore, we aim to describe this phenomenon and its associated risk factors in comparison with other sexual orientations and practices. A scoping review was carried out using the following databases: Scopus, Web of Science, Redalyc, Scielo.org, and Dialnet. The following keywords were used: same-sex, intragender, couple, domestic, and partner violence. The inclusion criteria applied were studies published between 2000 and 2022 with a minimum participation of 15% of Hispanic Americans, resulting in 23 articles. The findings showed a lower presence of studies on violence in women compared to men. Minority stress, power dynamics, social support, and childhood experiences of violence, which are related and complementary to each other, were identified as risk factors. We concluded that there is little research on IPV among women. In addition, studies require a renewed focus to comprehend this type of violence, which cannot be equated with those of heterosexual couples. This approach continues to perpetuate the invisibility of this problem, and, therefore, a more inclusive and specific perspective is needed.
The Influence of the COVID-19 Pandemic on Colorectal Cancer Secondary Preventive Healthcare Measures
Authors: Bărbulescu Linda-Nicoleta , Rădulescu Virginia-Maria , Bărbulescu Lucian-Florentin , Mogoantă Stelian-Ștefăniță
Published: September 2023

This study aims to assess the impact of SARS-CoV-2 on a population enrolled in a pilot colorectal screening program started by a family doctor in Romania. This observational retrospective study was spread over 43 months, respectively, from October 2019 to April 2023, and included 169 patients. The primary objective was to compare the pre-pandemic, pandemic, and post-pandemic periods to observe significant changes. The secondary objective was to study the correlation between vaccination against SARS-CoV-2 and participation in the study, especially in the age range class of interest—50–74 years. These data are important because Romanian Healthcare policymakers can use them to estimate the participation rate in a future national colorectal cancer screening and how to adjust and facilitate their communications with the targeted population. The rise of COVID-19 significantly negatively impacted the number of patients adhering to the pilot colorectal cancer screening and the number of test results during the pandemic period. However, in the post-pandemic period, the number of patients who joined the study, and the number of fecal occult blood tests was greater than expected (128.74%). We observed that screening participation was associated with vaccination (78.11% of patients had a complete COVID-19 vaccine scheme).
What Do Students’ Questionnaire Responses Tell Us about Their Language around Person-Centred Care? An Exploratory Sentiment Analysis
Authors: Wood Helen , Brand Gabrielle , Clifford Rhonda , Kado Sinead , Lee Kenneth , Seubert Liza
Published: September 2023

There is a global movement for health and social care to be person-centred: supporting people’s active participation when making health decisions and considering their opinions, beliefs, and needs. The World Health Organization recommend the inclusion of person-centred care in health and social care provision. This research aimed to explore Australian health and social care profession students’ language around person-centred care. Final-year health and social care professions students, attending one of two Australian universities, participated in an online questionnaire. Responses were analysed and themed to an existing person-centred care framework, then a sentiment analysis was applied to each response. Of the responses collected from 90 students, 235 statements were linked to the four core values of the person-centred care framework: cultivating communication (44%); respectful and compassionate care (35%); engaging patients in managing their care (20%); and integration of care (<1%). Within these, 24 statements were positively aligned (10%); 100 statements were neutral (43%); and 111 statements contained negative sentiments (47%). Almost half of the responses were not aligned with the core values of person-centred care. This suggests that many of the final-year students are not yet conceptualizing care using a person-centred approach.
Editorial for Special Issue “Maternal, Fetal and Neonatal Health”
Authors: Abdel-Latif Mohamed E.
Published: September 2023

The maternal, foetal, and neonatal health field has witnessed remarkable advancements in recent years, driven by cutting-edge research and innovative technologies [...]
Bridging the Gap: Exploring Opportunities, Challenges, and Problems in Integrating Assistive Technologies, Robotics, and Automated Machines into the Health Domain
Authors: Giansanti Daniele
Published: September 2023

The field of healthcare is continually evolving and advancing due to new technologies and innovations [...]
The Key Role of Empathy in the Relationship between Age and Social Support
Authors: Guariglia Paola , Palmiero Massimiliano , Giannini Anna Maria , Piccardi Laura
Published: September 2023

Aging involves several changes depending on genetic and behavioral factors, such as lifestyle and the number and quality of social relationships, which in turn can be influenced by empathy. Here, the change in the perceived social support across the lifespan as a function of empathy was investigated, considering the mediating role of empathy after controlling for gender and education. In total, 441 people (18–91 years old) filled in the Italian short version of the Interpersonal Support Evaluation List (ISEL-12), the Social Support Questionnaire (SSQ6), as well as the Empathy Questionnaire (EQ), and the Reading the Mind in the Eyes test (RMET). The mediation analyses with ISEL-12 showed that age and the EQ fully mediated the relationship between age and appraisal, belonging, and tangible scores. Further, the EQ fully mediated only the relationship between age and SSQ6-People. These results showed that empathic skills are key in the relationships between age and social support. This suggests that empathy can trigger social support and, ultimately, well-being if stimulated across the lifespan, especially from a young age; this would help to form the socio-emotional competence across the years as a sort of cushion that can be useful in the older to fulfill active aging.
Factors Affecting the Life Satisfaction of School-Aged Children with Vietnamese Immigrant Mothers in Korea
Authors: Cho Yoon-Hee , Lee Joohyun
Published: September 2023

Low life satisfaction among multicultural children is an important issue related to children’s mental health in Korea. The purpose of this study was to identify factors influencing the life satisfaction of children whose Vietnamese mothers migrated to Korea for marriage. Data from the Multicultural Adolescents Panel Study (MAPS) conducted by the National Youth Policy Institute (NYPI) in 2020 were used. The participants were 586 elementary school students. The mean age was 11.01 years (SD 0.19), and 52.0% were girls. Based on Engel’s biopsychosocial (BPS) model, biological factors (gender, physical health, and body mass index), psychological factors (mental health, acculturative stress, self-esteem, and general stress), and sociocultural factors (family economic status, social support, and parenting style) were measured and analyzed by using hierarchical multiple regression. In the final model, self-esteem (β = 0.399, p < 0.001), parental social support (β = 0.184, p < 0.001), and neglectful parenting style (β = 0.085, p = 0.031) were positively related to children’s life satisfaction, whereas general stress (β = −0.213, p < 0.001) and acculturative stress (β = −0.104, p = 0.002) were negatively related. The overall explanatory power was 41.7%. In conclusion, it is necessary to develop a specialized program that focuses on strengthening the bonds between parents and their children to enhance family functioning for multicultural families.
The Singapore Stone: Documenting the Origins, Destruction, Journey and Legacy of an Undeciphered Stone Monolith
Authors: Yap Kelvin Cahya , Jiao Tony (Wenyao) , Perono Cacciafoco Francesco
Published: September 2023

The Singapore Stone was a large monolith present at the mouth of the Singapore River, clad with a faded inscription that was a point of interest for local and foreign antiquarians and other enthusiasts, as no person—native or otherwise—could decipher the meaning of its tongue. Tragically, the stone was blasted in 1848 by East India Company engineers as part of works to widen the mouth of the river. Only four fragments were saved; these were sent to Calcutta’s Asiatic Society of Bengal and later placed in the custody of the Indian Museum. Today, only one fragment remains, which was returned to Singapore in 1919 and at present is displayed in the National Museum of Singapore. Over the past century and a half, there has been great interest in the fate of the lost fragments and in the mysterious inscription that the fragments hold. There have been various attempts at deciphering the Stone, with a variety of suggested interpretations and languages. This research paper compiles and documents both the physical journey of the fragments and the various attempts at deciphering them, aiming to comprehensively detail the Stone’s origins and journey from its erection to its present residence while providing an analysis of the past attempts at decipherment and the future of this effort.
Serratia marcescens Outbreak at a Correctional Facility: Environmental Sampling, Laboratory Analyses and Genomic Characterization to Assess Sources and Persistence
Authors: Ferguson Donna , Ryder Rahil , Lunsford Rawni , Dash Arie , Kamali Amanda , Kimura Akiko , Crandall John , Mukhopadhyay Rituparna , Dowless Heather , Ortiz Nancy , Jue Nathaniel K.
Published: September 2023

Serratia marcescens is an environmental bacterium and clinical pathogen that can cause an array of infections. We describe an environmental sampling and comparative genomics approach used to investigate a multi-year outbreak of S. marcescens at a correctional facility. Whole genome sequencing analysis revealed a predominant cluster of clonally related S. marcescens from nine patient cases and items associated with illicit drug use. Closely related strains found among items associated with case-patient cells and diluted Cell Block 64 (CB64), a quaternary ammonium disinfectant, and Break Out (BO), a multipurpose cleaner, highlighted their role as environmental reservoirs for S. marcescens in this outbreak. Comparative genomic analysis suggested outbreak strains were both persistent (identical strains found over long periods and in multiple locations of the correctional facility) and diverse (strains clustered with multiple global samples from NCBI database). No correlation was found between antimicrobial resistance (AMR) genes of outbreak strains; NCBI strains have more AMR genes. Principal component analysis (PCA) of virulence factors associated with persistence and infectivity indicated variation based on phylogroups, including the predominant cluster; identifiable variations among environmental versus clinical strains were not observed. Identification of multiple distinct genetic groups highlights the importance of putting epidemiological genomic studies in a proper genetic context.
Exploring Divergent Patterns and Dynamics of Urban and Active Rural Developments—A Case Study of Dezhou City
Authors: Zhong Huimin , Liu Zhengjia , Huang Yihang
Published: September 2023

Clarifying urban-rural spatial explicit structure changes is of great significance for understanding the urban-rural relationship evolution. Previous studies have mostly focused on urban internal spatial structure evolutions and less on the regional scale when it comes to exploring urban and rural evolutions. Nighttime light can timely reflect the human activities in regions and provides great potential for investigating the evolutions of urban and rural spatial explicit structures. Here, taking Dezhou City, a rapidly urbanizing city in China, as a case study, we employed the local contour tree method and nighttime light data to map urban and active rural extents from 2012 to 2020 and further explored their respective development processes. This study showed that unlike in rural regions, the internally explicit structures of urban regions were more complex, and there were often multiple hotspots inside them. The area of the urban-rural region increased significantly by 39.3% from 2012 to 2020 (p < 0.05). Populations were greatly responsible for the spatial explicit structure changes of urban and active rural regions. The urban and rural region rankings of the identified counties were basically consistent with the urban and rural population rankings. Unlike the perspectives of earlier land use (i.e., built-up land or impervious surface), this study underlined urban and active rural regions in view of the scope of active human activities. These results can likely help policymakers understand current active human activity extents and provide a data-based reference for future public services and infrastructure planning.