bluuebunny/crossref_metadata_2025 huggingface dataset:
MTT assay dataset of polyethylenimine coated graphenoxide nanosheets on breast cancer cell lines (MCF7, MDA-MB-231, MDA-MB-468)
Authors: Maleki Parichehr , Sadeghi Zahra , Shahryar Rahpeyma Sayyed , Taheri Mohammad , Raheb Jamshid
Published: March 2020

Graphen oxide has emerged as a promising tool in medical biotechnology due to its outstanding properties applicable in several fields as well as cell imaging, drug and gene delivery. Monolayer structure and high surface area of Graphen benefits elevated loading capacity of drugs rather than other nanomaterials. However Graphen oxide in physiological solutions has unfavourable reactions which confine it’s application in biomedical field without additional surface functionalization. Coating of graphenoxide by polyethylenimine is an approach to enhance biocompatibility of graphen oxide and also provides desirable physicochemical features for oligonucleotides delivery. The data presented here is related to graphenoxide-PEI characterisation and it’s cytotoxicity assay on variouse breast cancer cell lines including MDA-MB-468 and MDA-MB-231 and MCF7 by MTT assay.
Collaborative policy networks analysis in the policy-making process: Communal (Ulayat) land policy case in Riau Province, Indonesia
Authors: Marta Auradian , Suwaryo Utang , Sulaeman Affan , Agustino Leo
Published: March 2020

BACKGROUND: This research originated from empirical phenomena which showed that the result of communal land policies was not beneficial to indigenous people and led to lawsuits of interest groups and representatives of indigenous people. OBJECTIVE: This research examines the policy network in the policy-making process of communal land in Riau Province. We analysed the relationship between actors and each interest it carries to affect the policy process either in the policy implementation or policy formulation. METHODS: This research uses the qualitative approach with a case study method. This is an empirical inquiry that investigates phenomena in real life contexts where the boundaries between phenomena and contexts do not appear explicitly or clearly and makes use of various sources of evidence. RESULTS: The findings of the study show that although the composition of actors in communal land policy making in Riau Province have been heterogeneous, there are still limitations in identifying crucial issues faced by indigenous people. Meanwhile, there is also a change in the policy network at the stage of finalization and decision making which is limited to public actors or government. CONCLUSION: This study presented that the collaborative policy network determines the results of communal land policies in Riau Province. Collaborative policy network is not created, so policy outcomes are only determined by actors who are dominant in the policy network.
The current wave and determinants of brain-drain migration from China
Authors: Iqbal Kashif , Peng Hui , Hafeez Muhammad , Wang Yichu , Khurshaid  , Li Chenyu
Published: March 2020

BACKGROUND:

In the global competition for talent, the highly skilled and professional workers are increasingly being recognized as key drivers for innovation and economic prosperity.

OBJECTIVE:

The aim of this study was to investigate the wave and determinants of the brain-drain migration of skilled human capital from China.

METHODS:

We carried out a survey across a few rural and urban areas in China with 2,077 respondents by using a probability sampling technique. For empirical estimation, we employed the logistic regression estimation technique to compute and evaluate the data.

RESULTS:

The findings of this study suggest that high wages outside China and low wages within China is the top reason to move out of China. Additionally, more opportunities and better lifestyle away from China are key factors to push skilled human capital from China to host country. The findings of our study also indicate that better education abroad has a positive correlation with the brain-drain migration intention in China.

CONCLUSIONS:

The empirical results of the survey should be a high concern for the policy makers of China. Most survey respondents were young, well-educated and highly skilled, they identified several critical reasons that compel them to migrate from China.
Plasma Amyloid Concentration in Alzheimer’s Disease: Performance of a High-Throughput Amyloid Assay in Distinguishing Alzheimer’s Disease Cases from Controls
Authors: Feinkohl Insa , Schipke Carola G. , Kruppa Jochen , Menne Felix , Winterer Georg , Pischon Tobias , Peters Oliver
Published: March 2020

Background:

Collection of cerebrospinal fluid (CSF) for measurement of amyloid-β (Aβ) species is a gold standard in Alzheimer’s disease (AD) diagnosis, but has risks. Thus, establishing a low-risk blood Aβ test with high AD sensitivity and specificity is of outmost interest.

Objective:

We evaluated the ability of a commercially available plasma Aβ assay to distinguish AD patients from biomarker-healthy controls.

Method:

In a case-control design, we examined plasma samples from 44 AD patients (A + N+) and 49 controls (A–N–) from a memory clinic. AD was diagnosed using a combination of neuropsychological examination, CSF biomarker analysis and brain imaging. Total Aβ40 and total Aβ42 in plasma were measured through enzyme-linked immunosorbent assay (ELISA) technology using ABtest40 and ABtest42 test kits (Araclon Biotech Ltd.). Receiver operating characteristic (ROC) analyses with outcome AD were performed, and sensitivity and specificity were calculated.

Results:

Plasma Aβ42/40 was weakly positively correlated with CSF Aβ42/40 (Spearman’s rho 0.22; p = 0.037). Plasma Aβ42/40 alone was not able to statistically significantly distinguish between AD patients and controls (AUC 0.58; 95% CI 0.46, 0.70). At a cut-point of 0.076 maximizing sensitivity and specificity, plasma Aβ42/40 had a sensitivity of 61.2% and a specificity of 63.6%.

Conclusion:

In this sample, the high-throughput blood Aβ assay was not able to distinguish well between AD patients and controls. Whether or not the assay may be useful in large-scale epidemiological settings remains to be seen.
Optimal design for low iron-loss variable flux permanent magnet memory machine
Authors: Guo Liyan , Geng Qiang , Chen Wei , Wang Huimin
Published: March 2020

This paper proposes a kind of low iron-loss variable flux permanent magnet memory machine. The proposed memory machine can expand the speed range of machine, guarantee the operation efficiency of machine, avoid the irreversible demagnetization of PMs under high speed, and decrease the iron loss of the machine. Further, Taguchi method is used to optimize the proposed low iron-loss PM memory machine under magnetizing and demagnetizing current pulses respectively. Finally, the final optimum design scheme for the proposed low iron-loss variable flux permanent magnet memory machine is achieved by giving consideration to each optimization objective.
Analysing time pressure in professional tennis
Authors: Mlakar Miha , Kovalchik Stephanie Ann
Published: March 2020

Taking advantage of space and time is a major focus of tennis coaching yet few statistical measures exist to evaluate a player’s spatio-temporal performance in matches. The present study proposed the time to net as a single metric capturing both space and time characteristics of the quality of a shot. Tracking data from 2017 Australian Open allowed a detailed investigation of the characteristics and predictive value of the time-to-net in 33,913 men’s and 19,195 women’s shots. For groundstroke shots, the majority of men’s and women’s shots have a time-to-net between 200 and 800 ms. The expected time to net was found to vary significantly by gender, shot type, and where in a rally it occurred. We found considerable between-player differences in average time-to-net of groundstrokes when serving or receiving, indicating the potential for time-to-net to capture differences in playing style. Time-to-net increased prediction accuracy of point outcomes by 8 percentage points. These findings show that time to net is a simple spatio-temporal statistic that has descriptive and predictive value for performance analysis in tennis.
Prognostic Value of Histologic Subtype and Treatment Modality for T1a Kidney Cancers
Authors: Siev Michael , Renson Audrey , Tan Hung-Jui , Rose Tracy L. , Kang Stella K. , Huang William C. , Bjurlin Marc A.
Published: March 2020

Introduction:

To evaluate overall survival (OS) of T1a kidney cancers stratified by histologic subtype and curative treatment including partial nephrectomy (PN), percutaneous ablation (PA), and radical nephrectomy (RN).

Materials and Methods:

We queried the National Cancer Data Base (2004–2015) for patients with T1a kidney cancers who were treated surgically. OS was estimated by Kaplan-Meier curves based on histologic subtype and management. Cox proportional regression models were used to determine whether histologic subtypes and management procedure predicted OS.

Results:

46,014 T1a kidney cancers met inclusion criteria. Kaplan Meier curves demonstrated differences in OS by treatment for clear cell, papillary, chromophobe, and cystic histologic subtypes (all p < 0.001), but no differences for sarcomatoid ( p = 0.110) or collecting duct ( p = 0.392) were observed. Adjusted Cox regression showed worse OS for PA than PN among patients with clear cell (HR 1.58, 95% CI [1.44–1.73], papillary RCC (1.53 [1.34–1.75]), and chromophobe RCC (2.19 [1.64–2.91]). OS was worse for RN than PN for clear cell (HR 1.38 [1.28–1.50]) papillary (1.34 [1.16–1.56]) and chromophobe RCC (1.92 [1.43–2.58]). Predictive models using Cox proportional hazards incorporating histology and surgical procedure alone were limited (c-index 0.63) while adding demographics demonstrated fair predictive power for OS (c-index 0.73).

Conclusions:

In patients with pathologic T1a RCC, patterns of OS differed by surgery and histologic subtype. Patients receiving PN appears to have better prognosis than both PA and RN. However, the incorporation of histologic subtype and treatment modality into a risk stratification model to predict OS had limited utility compared with variables representing competing risks.
Improving timing of antibiotics in neonates with early onset sepsis – Quality improvement project
Authors: Ma Cheng , Levin Garret , Panda Sanjeet K. , Sambalingam Devaraj , Singh Ajay Pratap
Published: March 2020

BACKGROUND AND OBJECTIVES:

Early onset sepsis (EOS) is an important cause of neonatal morbidity and mortality. Timely administration of antibiotics is crucial in management. We initiated a quality improvement project to improve timely administration of antibiotics.

METHODS:

Primary drivers of change identified by the team were improving delivery of antibiotics from pharmacy and improving time to admit in the electronic medical record (EMR) in order to improve overall timeliness of antibiotics administration. Timings of antibiotics administration was tracked by using a control chart. Timings of antibiotics and outcomes of pre-intervention (December 2016) were compared with post intervention of PDSA cycles (January 2017–November 2018).

RESULTS:

There was statistically significant improvement in time to admission in electronic medical records over the time periods of pre-intervention, PDSA I and PDSA II ( p-value <  0.05) ( Table 1 ). Also, time to delivery of antibiotics from pharmacy was significantly reduced between PDSA cycles from 21 minutes to 9 minutes with improvement in overall workflow. An average time to infusion of antibiotics decreased from 70 minutes to 48 minutes. There was also overall improvement in number of neonates receiving antibiotics under 1 hour of decision making from 37% to 77%.

CONCLUSIONS:

In our study we were able to successfully implement our “antibiotics under one hour” goal. The ability to achieve this objective can be met across multi-institutions rendering care to newborns if the approach is multidisciplinary. Deleting obstructions in the process that involve admission, registration and entry into the EMR effectively reduced time.
Automatic left ventricle segmentation from cardiac magnetic resonance images using a capsule network
Authors: He Yangsu , Qin Wenjian , Wu Yin , Zhang Mengxi , Yang Yongfeng , Liu Xin , Zheng Hairong , Liang Dong , Hu Zhanli
Published: March 2020

PURPOSE:

Segmentation of magnetic resonance images (MRI) of the left ventricle (LV) plays a key role in quantifying the volumetric functions of the heart, such as the area, volume, and ejection fraction. Traditionally, LV segmentation is performed manually by experienced experts, which is both time-consuming and prone to subjective bias. This study aims to develop a novel capsule-based automated segmentation method to automatically segment the LV from images obtained by cardiac MRI.

METHOD:

The technique applied for segmentation uses Fourier analysis and the circular Hough transform (CHT) to indicate the approximate location of the LV and a network capsule to precisely segment the LV. The neurons of the capsule network output a vector and preserve much of the information about the input by replacing the largest pooling layer with convolutional strides and dynamic routing. Finally, the segmentation result is postprocessed by threshold segmentation and morphological processing to increase the accuracy of LV segmentation.

RESULTS:

We fully exploit the capsule network to achieve the segmentation goal and combine LV detection and capsule concepts to complete LV segmentation. In the experiments, the tested methods achieved LV Dice scores of 0.922±0.05 end-diastolic (ED) and 0.898±0.11 end-systolic (ES) on the ACDC 2017 data set. The experimental results confirm that the algorithm can effectively perform LV segmentation from a cardiac magnetic resonance image. To verify the performance of the proposed method, visual and quantitative comparisons are also performed, which show that the proposed method exhibits improved segmentation accuracy compared with the traditional method.

CONCLUSIONS:

The evaluation metrics of medical image segmentation indicate that the proposed method in combination with postprocessing and feature detection effectively improves segmentation accuracy for cardiac MRI. To the best of our knowledge, this study is the first to use a deep learning model based on capsule networks to systematically evaluate end-to-end LV segmentation.
Radiomics study for predicting the expression of PD-L1 in non-small cell lung cancer based on CT images and clinicopathologic features
Authors: Sun Zongqiong , Hu Shudong , Ge Yuxi , Wang Jun , Duan Shaofeng , Song Jiayang , Hu Chunhong , Li Yonggang
Published: March 2020

PURPOSE:

To predict programmed death-ligand 1 (PD-L1) expression of tumor cells in non-small cell lung cancer (NSCLC) patients by using a radiomics study based on CT images and clinicopathologic features.

MATERIALS AND METHODS:

A total of 390 confirmed NSCLC patients who performed chest CT scan and immunohistochemistry (IHC) examination of PD-L1 of lung tumors with clinic data were collected in this retrospective study, which were divided into two cohorts namely, training ( n = 260) and validation ( n = 130) cohort. Clinicopathologic features were compared between two cohorts. Lung tumors were segmented by using ITK-snap kit on CT images. Total 200 radiomic features in the segmented images were calculated using in-house texture analysis software, then filtered and minimized by least absolute shrinkage and selection operator (LASSO) regression to select optimal radiomic features based on its relevance of PD-L1 expression status in IHC results and develop radiomics signature. Radiomics signature and clinicopathologic risk factors were incorporated to develop prediction model by using multivariable logistic regression analysis. The receiver operating characteristic (ROC) curves were generated and the areas under the curves (AUC) were reckoned to predict PD-L1 expression in both training and validation cohorts.

RESULTS:

In 200 extracted radiomic features, 9 were selected to develop radiomics signature. In univariate analysis, PD-L1 expression of lung tumors was significantly correlated with radiomics signature, histologic type, and histologic grade ( p < 0.05, respectively). However, PD-L1 expression was not correlated with gender, age, tumor location, CEA level, TNM stage, and smoking ( p > 0.05). For prediction of PD-L1 expression, the prediction model that combines radiomics signature and clinicopathologic features resulted in AUCs of 0.829 and 0.848 in the training and validation cohort, respectively.

CONCLUSION:

The prediction model that incorporates the radiomics signature and clinical risk factors has potential to facilitate the individualized prediction of PD-L1 expression in NSCLC patients and identify patients who can benefit from anti-PD-L1 immunotherapy.
IPTEKS PENGENDALIAN INTERN DALAM PROSEDUR PENCAIRAN KREDIT KONSUMTIF PADA PT. BANK SULUTGO CABANG UTAMA
Authors: Maria Brigita , Chandra Vionalisa , Muliad Halim Ellena , Elim Inggriani
Published: March 2020

In conducting credit distribution to the public, there are several procedures that must be carried out before credit can be disbursed. Lending also needs to pay attention to internal controls within the company. The purpose of this study is to evaluate whether internal control in credit disbursement procedures is in accordance with the theory of control according to COSO. This research method is a qualitative method. Data collection techniques are interviews and documentation. The results of this study indicate that the company has not fully implemented internal controls based on COSO, particularly environmental control and monitoring.
自来水供水管理存在的问题及对策
Authors: 侯 桂兰
Published: March 2020

本文主要针对自来水供水管理存在的问题及对策展开深入研究,存在问题中,论述了供水规划较不科学、供水调度运行的经济性严重缺失、节水设备的问题较为突出等,然后有针对性地论述了几点完善对策,如制定供水规划方案、提高供水调度运行的经济性、加大供水管理工作力度、完善供水管理部门的管理体系、注重节水管理、提高供水管理人员的素质水平等,促进自来水供水管理工作的顺利进行,给予饮用水安全一定保证,避免自来水在供水管理方面出现问题。
对水利工程施工中堤坝防渗加固技术的运用研究分析
Authors: 刘 岗
Published: March 2020

水利工程中加固施工技术能够及时采取和现实情况相结合的方法提高工程质量,尽可能提高水的利用率以及产能量,促进水利业可持续发展。在当前水利工程建设施工的过程中,人们对水利堤坝工程的防渗施工工艺也越来越重视,因此我们在对其进行防渗加固施工的过程中,就要根据工程施工的实际情况来对其施工方法进行选择,从而保障水利工程的正常运行。本文对水利工程堤坝防渗加固施工中存在的问题,进行综合分析,希望能够为有关人员提供一定的借鉴与参考。
水利工程建筑设计的相关问题浅谈
Authors: 史 明
Published: March 2020

现阶段,完善水利建筑设计是一项长期性工作,准确把握时代发展的脉搏,调整水利工程建筑设计,在设计中实现建筑与生态景观的有机结合,成为设计人员关注的焦点。本文就将分析水利项目设计的目标、设计要点,并提出相应的改进策略,以供参考。
浅析给排水施工安全及给排水工程质量控制
Authors: 黄 静
Published: March 2020

由于给排水施工工程的质量和安全是影响居民日常生活质量的关键因素,在施工过程中管道噪声大,街道漏水,阀门松动等质量问题已成为影响给排水工程质量的重要因素。
简析水利水电工程中碾压混凝土大坝施工技术
Authors: 薛 艳蕾
Published: March 2020

水利工程主要用于控制和调配各类水资源,与国计民生息息相关。当前,人们对水利水电工程的施工要求不断提高,优化施工技术显得尤为重要。在水利水电工程施工过程中,优化碾压混凝土大坝施工技术,有利于提高大坝质量,促进工程安全运行。
立德树人视域下高职人才培养研究
Authors: 邓 韵雅
Published: March 2020

在中国经济和科技的快速发展过程中，对于高职院校培养创新型人才的迫切需求在逐渐提升。同时国家和学校对于高职学生创新素养的重视程度在不断提高，另一方面在高职院校中很多学生对创新理念还有一定的偏见。因此为了更好的加强培养高职院校创新型人才，必须将“立德树人”作为基本价值观进行工作引领，以关于核心素养的5C模型作为基本参考，进而提升高职创新人才培养工作的效率和质量。
Legal Regulation of Interaction of Security Service of Ukraine with Civil Society in Counteracting Terrorism
Authors: Helzhynskyi A. Yu.
Published: March 2020

The relevance of the research is determined by the prerequisites for the rise in the levels of terrorist threats in Ukraine and the development of international terrorism. In response to these threats, the state creates protective mechanisms, increasing the level of protection of the population, since this is one of its main tasks. However, this system does not fully meet its objectives without cooperation with civil society. That is why, one of the current tasks is to unite the efforts of the state and civil society institutions in fighting against terrorism.

Every citizen and civil society in the whole is a stakeholder and partner in the fight against terrorism. Citizens should be active participants. Therefore, the article provides a legal analysis of the interaction of the main agency in the state system of combating terrorist activity – the Security Service of Ukraine with civil society and clarifies the ways of implementing the state policy on counteracting terrorism.

The comparative legal method of the study analyzes the source base of national law concerning the interaction of the Security Service of Ukraine with civil society in fighting against terrorism.

The practical relevance of the study is determined by the ability to use the main points and conclusions of the study to further studies of the problems of the interaction of counter-terrorism actors with civil society.

As a result of the analysis of the normative base on the interaction of the Security Service of Ukraine with civil society in fighting against terrorism, it can be stated about the state's efforts to implement the main points of the UN Global Counter-Terrorism Strategy. In general, Ukraine's anti-terrorist system meets international standards in the field of counteracting terrorism. However, national legislation is the subject to improvement in the field of counteracting terrorism with the involvement of civil society institutions in eliminating the conditions and causes of terrorist activity.
The Concept and Essence of Administrative and Legal Protection of Tax Relations
Authors: Rohozinnikova K. S.
Published: March 2020

The concept and essence of administrative and legal protection of tax relations have been considered. It has been emphasized that the study of general theoretical ideas about the correlation between the concepts of legal security and legal protection will contribute to solving the tasks of the research. The provisions on the correlation of legal security and legal protection in the whole and as a part have been supported. The author has established peculiarities of administrative and legal protection compared with other types of legal protection of public relations: such activity is carried out by public administration agencies through administrative and legal means, including coercive ones. The essential components of administrative and legal protection, which should form the basis of its definition, include: prevention of negative phenomena; detection of possible violations; overcoming harmful consequences (restoration of violated rights); maintaining stable legal relations; prosecuting persons who encroach on the protected object.

The author has emphasized on the peculiarities of the purpose of using the means of administrative and legal protection of tax relations – protection of public interests in the field of taxation, as an organic and balanced combination of public and private interests, the satisfaction of which contributes to the sustainable development of society.

It has been found out that the content of administrative protection of a particular object is revealed through its means and measures. The analysis of sectoral studies has allowed to establish the following feature of administrative and legal protection measures: they are carried out by means of service nature, the list of which differs depending on the object of administrative legal protection, control and supervision, as well as by applying measures of administrative coercion.

The need for a two-vector consideration of administrative and legal protection means has been emphasized: their impact is directed both on the taxpayer and on the controlling agencies (revenue and fees agencies).
The contribution of self control to students’ discipline
Authors: Adlya Soeci Izzati , Yusuf A Muri , Effendi Mawardi
Published: March 2020

Good self control will lead individual to have discipline behavior. This research aims to describe the contribution of self control to students’ discipline. This research used a quantitative approach with a descriptive method. The subject of the research was 275 students. Scale was used to collect the data which consist of Self Control Scale and Students’ Discipline Scale. The data was analyzed by using descriptive statistic method and simple regression with SPSS version 20. The research findings shows significant contribution of self control to students’ discipline. Thus, self control gives significant impact to students’ discipline. This findings is based on data analysis which shows r score 0,590 means there is strong relationship between self control and students’ discipline.
Discriminação e preconceito linguístico: questões para promoção da saúde?
Authors: Falcão Ana Regina Graner , Souza Luiz Augusto de Paula , Costa Rogério da
Published: March 2020

As variedades linguísticas socialmente desprestigiadas em relação à chamada norma culta, podem configurar processos de discriminação e exclusão social em ambientes sociais diversos. A desqualificação social derivada da discriminação e do preconceito linguístico, quando naturalizada, pode sugerir que falantes discriminados tem dificuldades individuais de aprendizagem ou de saúde, por suposta alteração cognitiva, articulatória ou de comunicação. Este estudo partiu de situação concreta, vivenciada em clínica-escola universitária: procura de jovens e adultos com queixas relacionadas aos modos de falar, sob a premissa de que a discriminação desses modos poderia indicar agravo de saúde. O objetivo da pesquisa foi investigar e caracterizar essa demanda através de estudo de caso, com estratégia metodológica de Grupo de Discussão. Os resultados da pesquisa sugerem a existência de “patologização” das variações dos modos de falar, que levam a efeitos sociais e subjetivos com potencial para prejudicar a saúde, além das oportunidades de inserção social e econômica, e do exercício da cidadania. Situação que leva à indagação: não seria esta uma situação de vulnerabilidade a ser considerada em ações de promoção da saúde?

Palavras-chave: Promoção da Saúde, preconceito linguístico, vulnerabilidade em saúde.
Social Inequality among the Female Merchants of Ethnic Malay and Chinese
Authors: Ginting Eraskaita , Yusnaini Yusnaini
Published: March 2020

People in Jambi city assume that Pasar Hong Kong, a traditional market located in Jelutung sub-district, is “a Chinese market”, even some areas in the city of Jambi such as Jelutung, Koni, and Talang Banjar are dominated by the ethnic of Chinese. This research aims to explore how social inequality that occurs due to the advantages and disadvantages of an ethnic group so that it can affect individual attitudes that damage social capital. This research uses a case study approach with in-depth interviews and literature study as data collection techniques. The subjects of this study were ethnic Malay and Chinese female merchants in Pasar Hong Kong, Jambi city, Indonesia. This research found that social inequalities that occur among the female merchants of ethnic Malay and Chinese in Pasar Hongkong occur naturally, where both merchants and buyers have a high tolerance when interacting. Although sometimes there are differences in attitude when the merchants serve different ethnic buyers. The involvement of traditional leaders in managing social inequalities is very important due to the lack of assimilation among ethnic Malay and Chinese.
Identification of diatoms in a healthy Pennsylvania stream compared to three downstream sites impacted by Abandoned Mine Drainage
Authors: Arena Christopher M. , Galebach John L. , Mandichak Thomas M. , Engle J. Michael , Anderson Merrilee G.
Published: March 2020

Life in a healthy stream can be severely impacted by changes in pH and other water quality parameters. This study reveals differences in diatom diversity and water quality characteristics in a central Pennsylvania stream. One healthy site was compared to three nearby sites affected by abandoned mine drainage during a July sampling in 2013. Permanent slides were made and microscopically assessed for diatom identification. The healthy stream contained eleven diatom genera while the site most impacted by mine drainage showed only one diatom, Eunotia exigua. Data were analyzed for Shannon diversity index and species richness. Water samples showed differences in pH, aluminum, sulfate, and iron. This work demonstrates the use of diatoms as bioindicators of stream health.
Bdellovibrio bacteriovorus protects Caenorhabditis elegans from bacterial pathogens
Authors: Emmert Elizabeth A. B. , Haupt Zachary M. , Pflaum Katherine M. , Lasbury Jennifer L. , McGrath Justin P. , Collins Allison E. , Briand Christopher H.
Published: March 2020

Bdellovibrio bacteriovorus is a naturally predatory bacterium that multiplies inside Gram negative prey bacteria. There is much interest in using Bdellovibrio as a living antibiotic to control infections by Gram negative pathogens. In recent years Caenorhabditis elegans has proven to be an attractive animal model of bacterial pathogenesis for a range of pathogens. We have used the C. elegans animal pathogenesis model to examine the ability of B. bacteriovorus to protect nematodes from four bacterial pathogens. In all cases, nematodes treated with B. bacteriovorus and the pathogen survived at a significantly higher level than nematodes treated with the pathogen alone. Treatment with B. bacteriovorus alone was nontoxic to the worms. We monitored the persistence of E. coli K-12 and E. coli OP50 in both B. bacteriovorus treated nematodes and control nematodes. E. coli K-12 levels were significantly lower in B. bacteriovorus treated nematodes than in control nematodes one day after Bdellovibrio exposure and E. coli K-12 was eliminated from the worm gut two days faster in B. bacteriovorus treated nematodes. E. coli OP50 also demonstrated significantly lower levels in B. bacteriovorus treated nematodes and faster elimination from the worm gut. The successful use of B. bacteriovorus as a therapeutic agent in C. elegans indicates that it may be useful as a living antibiotic in other animal systems.
Detection of Borrelia and Ehrlichia in Rhipicephalus sanguineus
Authors: Vasquez-Espinoza Rosa , Beck David L.
Published: March 2020

Rhipicephalus sanguineus, the brown dog tick, is endemic throughout the world wherever domestic dogs are present. It has been recently reported by some veterinarians in the city of Laredo, Texas, USA, that Lyme disease, the most common tick-borne disease in the Northern United States, is present in local domestic dogs. Fully engorged R. sanguineus ticks were collected and their DNA was purified. The ticks were screened to determine the prevalence of Borrelia, Rickettsia and Ehrlichiaspecies. Sequences related to Borrelia burgdorferiin 9.8% (n=11/112), “Candidatus Borrelia lonestari” in 16.9% (n=19/112) and Ehrlichia canis in 12.5% (n=14/112) were detected by PCR. Sequencing has confirmed the presence of DNA from Ehrlichia canisand “Candidatus B. lonestari”, corroborating that Borrelia and Ehrlichia are present in domestic dogs in South Texas.
Frequency of Antibiotic Residues in a Central Wisconsin Dairy
Authors: Beilke Morgan L. , Fritz Jeffery D.
Published: March 2020

Antibiotics, used to maintain healthy dairy animals, persist in milk produced by treated animals for several days after therapy. Antibiotic residues, if present in the milk supply, negatively impact suitability for processing and consumption. This is an area of concern in the dairy industry and for the general public. This study explores the incidence of antibiotic residues in milk provided to a Central Wisconsin dairy. Random, unidentified samples were obtained over a four month period from producers located within a 100-mile radius of a dairy located in Granton, Wisconsin. All samples were tested within one week of their receipt, and maintained at refrigerated temperatures at all times prior to testing. Following testing, the origin of the samples was revealed. Samples were derived from two milk populations: those intended for human consumption (bulk milk), and those from cows under surveillance following antibiotic treatment (milk removed from the commercial supply). All bulk milk samples tested negative for over 55 different antibiotic residues, while all samples from the surveillance population tested positive for antibiotic residues. Our results are consistent with those observed nationally, and suggest that dairy producers recognize the ongoing concern of antibiotic contamination in the milk supply and are taking steps to prevent antibiotic contamination in milk. Our findings also suggest the public should have minimal concern with respect to antibiotic residues in the commercial milk supply.
Characterization of Indigenous Bacterial Communities in Crude-Oil Impacted Sites at Obagi Town, Onelga, Rivers State, Nigeria
Authors: Chikere Chioma Blaise , Ajuzieogu Chinyere Augusta , Miller Michael Chukwugoziem
Published: March 2020

Hydrocarbon utilizers are expected to be indigenous in crude-oil polluted environments. The isolation and characterization of hydrocarbon utilizers is often a key strategy in bioremediation of hydrocarbon-polluted environments. In this study, crude-oil polluted soil samples from Obagi town, Onelga, Rivers state were enumerated and characterized for putative hydrocarbon utilizing bacterial populations. Biochemical characterization identified five bacterial species representative of five genera: Bacillus, Pseudomonas, Acinetobacter, Micrococcus and Staphylococcus. Amongst the genera of bacteria isolated, Bacillus had the highest frequency of occurrence (40%). The mean count of total heterotrophic bacteria was 1.7 X 107 cfu/g, while hydrocarbon utilizing bacteria (HUB) count mean density was 1.0 X 107 cfu/g for the three soil samples. Statistical analyses revealed no significant difference at p>0.05 between Total Heterotrophic Bacterial (THB) and Hydrocarbon Utilizing Bacterial (HUB) counts, suggesting that most of the bacteria present in the sampled sites were hydrocarbon utilizers. Findings from this study suggest the presence of indigenous putative hydrocarbon utilizing bacteria in the crude-oil polluted soil of Obagi town. Hence, a promising potential exists for future bioremediation studies on the site.
Cutting wedge: bacterial community diversity and structure associated with the cheese rind and curd of seven natural rind cheeses
Authors: Wei Lei , Rubenstein Rebecca J. , Hanlon Kathleen M. , Wade Heidi , Peterson Celeste N. , Klepac-Ceraj Vanja
Published: March 2020

The microorganisms that inhabit cheese contribute greatly to the flavor and development of the final product. While the rind and curd microbiota have been characterized separately, there is limited information on how the structure and function of microbial communities in rinds and curds vary within and amongst cheeses. To better understand the differences in community structure and function between communities of cheese rinds and curds, we combined culture-based methods with culture-independent community profiling of curds and rinds. Rinds contained greater taxonomic diversity than curds. Lactobacillales dominated curd communities while members from the order Actinomycetales were found in high abundance in rind communities. Communities varied more between rinds and curds than among cheeses produced from different milk types. To better understand microbial community functions, we cultured and assayed isolates for antibiotic susceptibility and carbon source utilization. Among European and U.S. cheeses, 70% of all susceptible isolates were cultured from U.S. cheeses. Overall, our study explored the differences within and between rind and curd microbial communities of natural rind cheeses, provided insights into the environmental factors that shape microbial communities, and demonstrated that at the community and isolate level the cheese microbiome was diverse and metabolically complex.
Characterization of prodiginine compounds produced by a Vibrio species isolated from salt flat sediment along the Florida Gulf Coast
Authors: Morgan Stephanie , Thomas Matthew J. , Walstrom Katherine M. , Warrick Eric C. , Gasper Brittany J.
Published: March 2020

Prodiginines are secondary metabolites produced by several known species of bacteria. These metabolites are known for their bright pigmentation and their potential medicinal uses. Biosynthesis of prodiginine compounds, including the well-studied prodigiosin, has been well characterized in Serratia marcescens and other bacterial species, including several marine bacteria. In an effort to isolate and identify natural products from marine organisms, an environmental sample was taken from a salt flat along the Florida Gulf Coast and cultured for bacterial growth. A bacterial species that produces a vibrant pink pigment was isolated and identified as a member of the Vibrio genus and was named MI-2. Whole genome sequencing identified a 13-gene operon with homology to the S. marcescens prodigiosin biosynthetic operon. The pigment produced by MI-2 was hypothesized to be composed of prodigiosin or related prodiginine compounds and was purified by flash column chromatography and identified by mass spectrometry.
Molecular Pathogenesis of Bacillus spp., with Emphasis on the Dairy Industry
Authors: Grutsch Alyssa A. , Nimmer Pierre S. , Pittsley Rachel H. , Kornilow Katherine G. , McKillip John L.
Published: March 2020

The bacterial species Bacillus cereus accounts for 1.4-12% of foodborne illness outbreaks worldwide, a statistic that is certainly an underestimate. This bacterial genus is capable of contaminating a wide range of food products, including rice, chicken, vegetables, spices, and dairy products. B. cereus endospores are partially resistant to pasteurization, dehydration, gamma radiation, and other physical stresses used in food processing, and their adhesive characteristics promote biofilm-forming capability on a variety of substrates in dairy operations. B. cereus and other closely-related species produce several types of exotoxins, including at least four hemolysins, three phospholipases, a heat/acid stable emetic toxin called cereulide, and three well-studied heat-labile enterotoxins that all cause gastroenteritis following ingestion. While a great deal of information on virulence gene presence and expression is known in B. cereus, very little has been done to explore the virulence potential of thermoduric spore-formers that may be found in ultrahigh temperature (UHT) pasteurized milk, and their ability to produce biofilms. Biofilm production is understood to be under similar regulation as toxins and other extracellular virulence determinants. This chapter describes the current status of knowledge with Bacillus spp. relevant to the dairy industry, virulence potential, and biofilm production from the perspective of food safety.
浅析城市轨道交通建设工程质量管理
Authors: 董 延鹏
Published: March 2020

近年来，在我国社会经济快速发展的推动下，使得我国城市化发展取得了显著的成绩，城市轨道交通项目从某种层面上能够反映出一个城市的发展状态，但是就现如今我国城市轨道交通工程实际情况来说，整体质量方面还存在诸多的问题需要进一步的加以解决，正是这些质量问题的存在往往会造成施工单位的经济损失，并且也不利于轨道交通事业稳定健康发展。鉴于此，我们需要加大力度针对城市轨道交通工程质量加以管控，从根本上保证城市轨道工程质量。这篇文章主要围绕城市轨道交通建设工程质量管理工作展开全面分析研究，希望能够对城市轨道交通建设工作的良好发展有所助益。
地理信息大数据在国土空间规划中的应用研究
Authors: 王 骞
Published: March 2020

在当前时期，科技发展的速度持续加快，很多的行业已经对大数据予以应用，这就使得自身的发展更为稳健。我们国家对国土空间规划是十分重视的，此项工作和国民基础建设存在紧密的关联性，在展开生态文明建设时，此项工作也是十分关键的，所以说，必须要针对数据规划展开更为深入的研究，这样方可使得空间规划能够获得更为坚实的理论基础。对地理信息大数据展开分析时，将“3S”之类的技术予以充分应用，可以使得规划工作有序展开，预期目标也能够切实达成。
建筑工程管理中信息化的应用探讨
Authors: 顾 高峰 , 严 荣林
Published: March 2020

在当前时期，信息化技术已经在很多领域中得到应用，并使得大家的生活出现很大变化。从建筑工程来说，在展开管理工作时，若想使得管理更具实效性，应该要充分利用信息化技术，这样可以使得工程管理变得更为完善，同时可使得管理更具系统，这样一来，管理过程中发生问题的几率就会变得较低，企业所具有的竞争实力也会得到增强，并能够紧跟社会发展脚步。
浅谈改进农村生态环境促进生态农业经济发展
Authors: 崔 玉芳
Published: March 2020

农业在国家经济发展和综合国力的提升方面的作用是十分巨大的，其不仅与民众的生活密切相关，并且是社会发展的重要基础。在当前经济快速发展的趋势下，农业发展务必要进行不断的改革发展，全面引入最前沿的生产技术，全面落实生态农业，促进农业产量不断增加，为国家健康发展创造良好的基础。
THE ESSENCE AND INTERRELATION OF THE BASIC CONCEPTS OF THE THEORY OF CHANGE MANAGEMENT IN EDUCATION IN THE PHILOSOPHICAL AND SOCIAL ASPECT.
Authors: Bodnar Oksana , Horishna Oksana
Published: March 2020

Abstract. The formation of competitive ability of our state on the world stage led to the development of change management theory, in particular, in the educational field. The modernization of education is impossible without understanding the philosophical and social aspects of differentiation of the conceptual apparatus in the theory of change management. The urgency of the problem is enshrined in such normative documents as the Law of Ukraine on Education and the Concept of the New Ukrainian School.

The philosophy of change pervades both classical and modern philosophical sciences, in which the leading idea is the general variability of events. In the social context, scientists will highlight the problem of managing human life in the environment, which is directly the task of education.

Today, the educational institution is managed in line with changes. It has been investigated that the philosophical aspects of change management relate to particular concepts. It is substantiated that the concepts of «change» and «development», «process» and «evolution», «change» and «innovation» are in a dialectical connection to the extent of development of society. However, these concepts cannot substitute each other

Project developments specialize in the practical management of changes in educational institutions that apply and deepen the change of a single apparatus in philosophical and social work, which allows to shift a deeper understanding of the integral essential management processes.

The prospect of further research is the study of the social effects of applying change management mechanisms in general secondary education.
APPLICATION OF INNOVATION METHODS AND TECHNOLOGIES DURING TEACHING ECONOMIC DISCIPLINES IN PROFESSIONAL TRAINING OF FUTURE ENGINEERS
Authors: Bobrikova Julia
Published: March 2020

Abstract. The article analyzes the use of innovative methods and technologies in the process of preparation of the future engineer-teacher and determines and substantiates the influence of the use of innovative methods and technologies on the development of pedagogical skills of future engineers-pedagogues - participants of innovative processes, improvement of qualitative indicators of teaching methods and use technologies for future professionals.
The relevance of the use of methods and technologies in the teaching of economic disciplines in the process of professional training of future engineer-teacher, which are to teach the future specialist to independently acquire knowledge and apply them to solve new cognitive and practical problems; promote the development of communication skills; expand the circle of communication; to foster the ability to use research techniques: to collect and analyze information, to make hypotheses, to draw conclusions. The methods of interactive learning and their organizational and methodical forms are considered, which are the most optimal for the use of certain types of training in the preparation of specialists.
The role of innovative methods and technologies in shaping future professionals' professional readiness, thinking and behavior has been clarified.
The complex of professionally significant qualities of future engineers-educators necessary for successful professional activity is characterized and special attention is given to modern information technologies, their opportunities for improvement of professional skills.
The conclusions are drawn and the prospects for further research within the given direction are indicated.
SPECIAL FEATURES OF FORMING THE READINESS OF FUTURE MILITARY PILOTS FOR PROFESSIONAL INTERACTION WHEN USING THE COMMON AIR SPACE
Authors: Bratslavska Alla
Published: March 2020

Abstract. The article is devoted to the special features of the military pilots training and the problem of forming their readiness for professional interaction when using the joint airspace with other countries. The article deals with the specifics of a military pilot’s job and the specificity of military pilots training at the Kharkov National Air Force University, in particular, at the Aviation English Department. The components of military pilots' readiness for professional interaction, criteria and levels of such readiness are determined.
It is emphasized that readiness for professional interaction in the common airspace is a necessary requirement today in view of the active development of cooperation and formation of interoperability between Ukrainian aviation and foreign partners. Such readiness is impossible without a proper level of military pilots’ foreign language command. Successful formation of future aviation pilots' readiness for professional interaction while using common airspace requires the modernization of cadets training and the use of innovative pedagogical technologies that promote personality development and increase the training effectiveness.
EVALUATION OF PEDAGOGICAL ACTIVITY OF TEACHERS OF THE GENERAL SECONDARY EDUCATION AS AN ELEMENT OF THE INTERNAL QUALITY ASSURANCE SYSTEM
Authors: Grygorash Viktor
Published: March 2020

Abstract. The Law of Ukraine “On Education” provides for the establishment of an internal quality assurance system for education, which in particular may include criteria, rules and procedures for evaluating pedagogical activity. Therefore, a topical issue is the development of a number of internal documents that regulate both the internal audit processes in general and the objective evaluation of pedagogical activity both during the period of certification and within the operational monitoring of educational activities.
To a certain extent the requirements for pedagogical activity, some means of its assessment, an indicative list of activities that are subject to study, defined by the current regulatory documents, in particular, the Model provisions about certification of pedagogical staff. This provision provides for the study of pedagogical activity while attending classes, taking into account the level of educational achievement of students, studying pedagogical documentation, analysis of the methodological work of the teacher, etc. It is envisaged to evaluate the employee's performance of their duties, professional and personal qualities (initiative, competence, organization, etc.). Instead, the mechanism for assessing such different areas of educational activity is determined by each institution on its own, which leads to significant differences between the requirements for a specialist of a certain category in different institutions of general secondary education.
The Draft Law of Ukraine "On Complete General Secondary Education" defines the existence of an internal quality assurance system in educational institutions, which is formed by the educational institution taking into account the methodological recommendations developed by the State Service for Quality of Education of Ukraine. These recommendations include, in particular, the evaluation of areas such as the effectiveness of planning pedagogical activities, the use of modern educational approaches to the organization of the educational process, the system of professional development, cooperation with participants in the educational process, compliance with academic integrity and more. These relatively new criteria require significant changes in the internal system of assessment of pedagogical activity, necessitating the development of appropriate tools and mechanisms.
The article examines the level of research of the identified problem and emphasizes the need to create a standardized procedure for evaluating pedagogical activity in the institution of general secondary education (ZSSO). Certain approaches and tools, in particular qualimetric models, are proposed to evaluate the pedagogical performance of general secondary education staff.