bluuebunny/crossref_metadata_2025 huggingface dataset:
Biosafety for Dental Patients During Dentistry Care After COVID-19: A Review of the Literature
Authors: Siles-Garcia Adriana Abigail , Alzamora-Cepeda Anais Gabriela , Atoche-Socola Katherine Joselyn , Peña-Soto Claudio , Arriola-Guillén Luis Ernesto
Published: July 2020

The world is currently changing due to coronavirus disease 2019 (COVID-19), and the field of dentistry is no stranger to this. The care of patients in the dental office involves very strict biosafety protocols, and patients must be aware of the protection barriers implemented to allow satisfactory, safe dental care. The purpose of this study was to synthesize and analyze the management of the current biosafety standards for dental patients since the arrival of the COVID-19 pandemic. A bibliographic search of the main sources of information including MEDLINE (by means of PubMed), Scopus, Science Direct, SCIELO, and Google Scholar was carried out. Articles published without language restriction, systematic reviews, literature reviews, and observational studies were included. We identified the biosafety measures that must be taken before, during, and after dental practice following the arrival of COVID-19. The main measures include telephone triage, temperature taking on arrival at the office, the organization of the waiting room, washing hands before entering the office, knowing the auxiliary radiographic exams of choice and what type of treatment can be performed, albeit with restrictions. In conclusion, dental patients must comply with all the biosafety measures established by international protection standards and implemented by dentists before, during, and after dental practice to reduce the possibility of COVID-19 infection.
Slavs and Snakes: Material Markers of Elite Identity in Viking Age Poland
Authors: Gardeła Leszek , Kajkowski Kamil
Published: July 2020

This article offers a new perspective on a diverse corpus of high-status Western Slavic objects from the domain of the Piast dynasty in Poland, dated between the tenth and eleventh centuriesad. It is proposed that the lavish zoomorphic decorations, often depicting snakes, found on jewellery, weapons, and equestrian equipment reflected Western Slavic pre-Christian religious ideas and served as material markers of elite identity. The results of this study lead to a more nuanced understanding of Western Slavic worldviews and their material expressions, paving the way for new investigations into cultural interactions both within and beyond the Slavic homelands.
Increasing Legal Certainty without Trust: Why Regulation 2019/515 Cannot Achieve the Unachievable
Authors: JAN Benjamin
Published: July 2020

More than 40 years after Cassis de Dijon, the mutual recognition in the field of goods is still a failure. The promise of this principle for ensuring both market access and regulatory diversity has not been kept. Therefore, today, businesses rarely rely on mutual recognition to sell their products in another Member State. In an attempt to stimulate this procedure further, the European Union legislator tried to simplify the procedures to be followed by businesses and public administrations through Regulation 2019/515. This article argues that, although the Regulation creates more legal certainty, it fundamentally fails to address the underlying problem of lack of trust that has stalled mutual recognition in the past.
ON THE SOLELY JURISDICTIONAL READING OF NONESTABLISHMENT
Authors: Gamwell Franklin I.
Published: July 2020

On the solely jurisdictional reading, the nonestablishment clause in the US Constitution's First Amendment was designed to confirm that power over politics in relation to religion was assigned solely to the several states. This article first summarizes two presentations of that view (those of Steven D. Smith and Akhil Reed Amar), offers a critique of it, and then outlines an alternative. The critique is theoretical, seeking to show the incoherence of the solely jurisdictional reading, such that any theorist who assumes its internal consistency cancels her or his own interpretation of the First Amendment. This incoherence is present because that reading assumes the suprarational character of religious or comprehensive convictions, even while those citizens who hold any such conviction believe that justice depends on the ultimate terms of political evaluation they affirm. On the alternative outlined, religious freedom makes sense if and only if the ultimate terms of evaluation are given in common (adult) human experience, and thus the question about them is itself rational.
The State-Owned Enterprise as an Identity: The Influence of Institutional Logics onGuanxiBehavior
Authors: Chen Xi
Published: July 2020

Previous research has debated whetherguanxipersists or declines with the development of formal institutions. This study addresses this debate by investigating how the development of formal institutions in China's state-owned organizations influences employees’guanxibehavior. Building on institutional logics theory, I propose thatguanxibehavior is a reaction to the socialist institutions adopted by state-owned enterprises (SOEs) and is associated with the collective identity of SOEs. Thus, employees’ identification with SOEs is the mechanism that influences theirguanxibehavior. A survey of 721 employees from 12 organizations comparedguanxibehavior across three types of organizations with different degrees of state ownership: SOEs, public firms, and joint ventures. The results showed that the employees of joint ventures identify less with SOEs and engage in lessguanxibehaviors than do SOE employees. The employees of public firms still identify with SOEs, and theirguanxibehavior did not differ from that of SOE employees. Identification with SOEs mediated the effect of organizational type onguanxibehavior, whereas formalization did not. Therefore, the development of formal institutions does not necessarily decreaseguanxibehavior, and its effect depends on whether the collective identity underlyingguanxiis changed. This study has important implications forguanxiresearch, institutional logics theory, and transition economies.
Structural equation modeling of the associations between amygdala activation, personality, and internalizing, externalizing symptoms of psychopathology
Authors: Neumann Craig S.
Published: July 2020

There is an expanding literature on the theoretical and empirical connections between personality and psychopathology, and their shared neurobiological correlates. Recent cybernetic theories of personality and psychopathology, as well as affective neuroscience theory, provide grounding for understanding neurobiological–personality–psychopathology (NPP) associations. With the emergence of large sample datasets (e.g., Human Connectome Project) advanced quantitative modeling can be used to rigorously test dynamic statistical representations of NPP connections. Also, research suggests that these connections are influenced by sex, and large samples provide the opportunity to examine how NPP associations might be moderated by sex. The current study used a large sample from the Duke Neurogenetics Study (DNS) to examine how amygdala activation to facial expressions was linked with self-report of personality traits and clinical interviews of internalizing and externalizing symptoms of psychopathology. Structural equation modeling results revealed direct associations of amygdala activation with personality trait expression, as well as indirect associations (though personality) with symptoms of psychopathology. Moreover, the NPP links were moderated by sex. The current results are in line with research that identifies a broader role played by the amygdala in personality and provide potential insights for continued research in personality neuroscience and recent theories on the neurobiology of personality.
Vegetation over the last glacial maximum at Girraween Lagoon, monsoonal northern Australia
Authors: Rowe Cassandra , Wurster Christopher M. , Zwart Costijn , Brand Michael , Hutley Lindsay B. , Levchenko Vladimir , Bird Michael I.
Published: July 2020

Northern Australia is a region where limited information exists on environments at the last glacial maximum (LGM). Girraween Lagoon is located on the central northern coast of Australia and is a site representative of regional tropical savanna woodlands. Girraween Lagoon remained a perennial waterbody throughout the LGM, and as a result retains a complete proxy record of last-glacial climate, vegetation and fire. This study combines independent palynological and geochemical analyses to demonstrate a dramatic reduction in both tree cover and woody richness, and an expansion of grassland, relative to current vegetation at the site. The process of tree decline was primarily controlled by the cool-dry glacial climate and CO2effects, though more localised site characteristics restricted wetland-associated vegetation. Fire processes played less of a role in determining vegetation than during the Holocene and modern day, with reduced fire activity consistent with significantly lower biomass available to burn. Girraween Lagoon's unique and detailed palaeoecological record provides the opportunity to explore and assess modelling studies of vegetation distribution during the LGM, particularly where a number of different global vegetation and/or climate simulations are inconsistent for northern Australia, and at a range of resolutions.
A Framework for Measuring Leaders’ Willingness to Use Force
Authors: CARTER JEFF , SMITH CHARLES E.
Published: July 2020

Political leaders’ willingness to use force is central to many explanations of foreign policy and interstate conflict. Unfortunately, existing indicators typically measure one aspect of this general concept, have limited coverage, and/or are not derived independently of leaders’ participation in interstate conflicts. We develop a strategy for constructing measures of leaders’ underlying willingness to use force with data on their background experiences, political orientations, and psychological traits in a Bayesian latent variable framework. Our approach produces measures of latent hawkishness for all national leaders between 1875 and 2004 that offer advantages over existing proxies along multiple dimensions, including construct validity, predictive validity, and measurement uncertainty. Importantly, our statistical framework allows scholars to build upon our measures by incorporating additional data and altering the assumptions underlying our models.
Increasing dietary n-6 fatty acids while keeping n-3 fatty acids stable decreases EPA in polar lipids of farmed Atlantic salmon (Salmo salar)
Authors: Hundal Bjørg K. , Liland Nina S. , Rosenlund Grethe , Bou Marta , Stubhaug Ingunn , Sissener Nini H.
Published: July 2020

There is an increased use of vegetable oils containing n-6 fatty acids (FA) in aquafeeds, and several trials indicate that there might be an increased requirement of EPA and DHA for Atlantic salmon when they are fed higher dietary n-6 FA. With a limited supply of EPA and DHA for production of aquafeeds, it is important to know how to efficiently use these FA to maintain growth and health of the fish. In the present trial, three diets containing equal amounts of n-3 FA (about 7·7 % of total FA) and different n-6:n-3 FA ratios (about 1, 2 and 6), as well as one diet with n-6:n-3 FA ratio at about 1 but twice as much n-3 FA, were fed to Atlantic salmon. Despite constant dietary n-3, increasing dietary n-6 led to significantly reduced n-3 in tissue polar lipids. Interestingly, EPA was significantly reduced while DHA was not. Maintaining a stable n-3 content in the polar lipids when increasing dietary n-6 FA was only obtained by simultaneously increasing the dietary n-3 content and with this maintaining the same n-6:n-3 FA ratio. Polar lipid n-6 FA in tissues thus primarily reflected the dietary n-6:n-3 FA ratio and not the absolute dietary n-6 FA content. Neutral lipids, on the other hand, reflected the dietary absolute levels of both n-3 and n-6 FA. This study indicates that a better use of dietary EPA is achieved by keeping the dietary n-6:n-3 FA ratio low.
Daily mycoprotein consumption for 1 week does not affect insulin sensitivity or glycaemic control but modulates the plasma lipidome in healthy adults: a randomised controlled trial
Authors: Coelho Mariana O. C. , Monteyne Alistair J. , Dirks Marlou L. , Finnigan Tim J. A. , Stephens Francis B. , Wall Benjamin T.
Published: July 2020

Mycoprotein consumption has been shown to improve acute postprandial glycaemic control and decrease circulating cholesterol concentrations. We investigated the impact of incorporating mycoprotein into the diet on insulin sensitivity (IS), glycaemic control and plasma lipoprotein composition. Twenty healthy adults participated in a randomised, parallel-group trial in which they consumed a 7 d fully controlled diet where lunch and dinner contained either meat/fish (control group, CON) or mycoprotein (MYC) as the primary source of dietary protein. Oral glucose tolerance tests were performed pre- and post-intervention, and 24 h continuous blood glucose monitoring was applied throughout. Fasting plasma samples were obtained pre- and post-intervention and were analysed using quantitative, targeted NMR-based metabonomics. There were no changes within or between groups in blood glucose or serum insulin responses, nor in IS or 24 h glycaemic profiles. No differences between groups were found for 171 of the 224 metabonomic targets. Forty-five lipid concentrations of different lipoprotein fractions (VLDL, LDL, intermediate-density lipoprotein and HDL) remained unchanged in CON but showed a coordinated decrease (7–27 %; all P < 0·05) in MYC. Total plasma cholesterol, free cholesterol, LDL-cholesterol, HDL2-cholesterol, DHA and n-3 fatty acids decreased to a larger degree in MYC (14–19 %) compared with CON (3–11 %; P < 0·05). Substituting meat/fish for mycoprotein twice daily for 1 week did not modulate whole-body IS or glycaemic control but resulted in changes to plasma lipid composition, the latter primarily consisting of a coordinated reduction in circulating cholesterol-containing lipoproteins.
Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade
Authors: Dubey J. P. , Pena H. F. J. , Cerqueira-Cézar C. K. , Murata F. H. A. , Kwok O. C. H. , Yang Y. R. , Gennari S. M. , Su C.
Published: July 2020

Toxoplasma gondiiinfections are common in humans and animals worldwide. Domestic free-range chickens (Gallus domesticus) are excellent sentinels of environmental contamination withT. gondiioocysts because they feed on the ground. Chickens can be easily infected withT. gondii; however, clinical toxoplasmosis is rare in these hosts. Chickens are comparatively inexpensive and thus are good sentinel animals forT. gondiiinfections on the farms. Here, the authors reviewed prevalence, the persistence of infection, clinical disease, epidemiology and genetic diversity ofT. gondiistrains isolated from chickens worldwide for the past decade. Data on phenotypic and molecular characteristics of 794 viableT. gondiistrains from chickens are discussed, including new data onT. gondiiisolates from chickens in Brazil. This paper will be of interest to biologists, epidemiologists, veterinarians and parasitologists.
An examination of reciprocal associations between substance use and effortful control across adolescence using a bifactor model of externalizing symptoms
Authors: Paige Katie J. , Meisel Samuel N. , Colder Craig R.
Published: July 2020

Early adolescence is thought to represent a window of vulnerability when exposure to substances is particularly harmful, partly because the neurotoxic effects of adolescent substance use may derail self-regulation development. However, previous studies fail to account for externalizing symptoms, such as aggression and delinquency, that accompany adolescent substance use and may also derail the development of self-regulation. The current study aims to clarify whether the neurotoxic effects of adolescent substance use are associated with deficits in effortful control (EC) after accounting for externalizing symptoms and to examine reciprocal relationships between EC, externalizing symptoms, and substance use. A longitudinal sample of adolescents (N = 387) was used to estimate bifactor models of externalizing symptoms across five assessments (Mage = 11.6 to 19.9). The broad general externalizing factors were prospectively associated with declines in EC across adolescence and emerging adulthood. However, the narrow substance use specific factors were not prospectively associated with EC. Findings suggest that the broader externalizing context, but not the specific neurotoxic effects of substance use, may hamper self-regulation development. It is critical to account for the hierarchical structure of psychopathology, namely externalizing symptoms, when considering development of EC.
The evolution process and the cause of the spatial distribution of cinemas in the Shanghai settlements (1919–1943)
Authors: Zhao Weiqing
Published: July 2020

In the first decades of the twentieth century, Shanghai became China's most important film centre, for both production and distribution. The city hosted the largest number of movie theatres in the country. After the establishment of the first theatre in 1908 by a Spanish national, the distribution of movie theatres gradually transferred and expanded from Hongkew District, north of Suzhou Creek to the south bank of the International Settlement. This article examines the characteristics of the distribution of movie theatres in the city from the perspective of authorities’ policy, population density, population structure, traffic and cultural space. It analyses the factors leading to the particular geographical distribution and discusses the possible links between the space of cinema, urban development and urban cultural space.
Lost in the New Deal Realignment: GI Joe
Authors: Norpoth Helmut
Published: July 2020

Military service in World War II produced a generation of Democrats. This finding results from an examination of Gallup polls (1945–1953) that probed both party identification and wartime service. The 1944 election afforded soldiers an opportunity to vote for their commander in chief, and they did so by a large margin for Franklin D. Roosevelt—a Democrat. A vote under these circumstances is bound to leave lifelong marks on a cohort in its impressionable years, which was the life stage of many World War II soldiers. Further tests rule out the possibility that the Democratic tendency of soldiers was simply the result of their youthful age, lower socioeconomic status, urban background, union membership, race, or Southern region—all of which predict partisanship. Neither did the return to civilian life erode the Democratic edge of veterans. GI Joe is an unsung hero of what is widely known as the New Deal realignment.
Interaction between Alcohol Consumption and Apolipoprotein E (ApoE) Genotype with Cognition in Middle-Aged Men
Authors: Slayday Riki E. , Gustavson Daniel E. , Elman Jeremy A. , Beck Asad , McEvoy Linda K. , Tu Xin M. , Fang Bin , Hauger Richard L. , Lyons Michael J. , McKenzie Ruth E. , Sanderson-Cimino Mark E. , Xian Hong , Kremen William S. , Franz Carol E.
Published: July 2020

Objective:

Heavy alcohol consumption is associated with poorer cognitive function in older adults. Although understudied in middle-aged adults, the relationship between alcohol and cognition may also be influenced by genetics such as the apolipoprotein (ApoE) ε4 allele, a risk factor for Alzheimer’s disease. We examined the relationship between alcohol consumption, ApoE genotype, and cognition in middle-aged adults and hypothesized that light and/or moderate drinkers (≤2 drinks per day) would show better cognitive performance than heavy drinkers or non-drinkers. Additionally, we hypothesized that the association between alcohol use and cognitive function would differ by ApoE genotype (ε4+ vs. ε4−).

Method:

Participants were 1266 men from the Vietnam Era Twin Study of Aging (VETSA; M age = 56; range 51–60) who completed a neuropsychological battery assessing seven cognitive abilities: general cognitive ability (GCA), episodic memory, processing speed, executive function, abstract reasoning, verbal fluency, and visuospatial ability. Alcohol consumption was categorized into five groups: never, former, light, moderate, and heavy.

Results:

In fully adjusted models, there was no significant main effect of alcohol consumption on cognitive functions. However, there was a significant interaction between alcohol consumption and ApoE ε4 status for GCA and episodic memory, such that the relationship of alcohol consumption and cognition was stronger in ε4 carriers. The ε4+ heavy drinking subgroup had the poorest GCA and episodic memory.

Conclusions:

Presence of the ε4 allele may increase vulnerability to the deleterious effects of heavy alcohol consumption. Beneficial effects of light or moderate alcohol consumption were not observed.
Quantitative Flow‐Ecology Relationships Using Distributed Lag Nonlinear Models: Large Floods in the Murray River Could Have Delayed Effects on Aquatic Macroinvertebrates Lasting More Than Three Decades
Authors: Le Chi T. U. , Paul Warren L. , Gawne Ben , Suter Phillip J.
Published: July 2020

We aimed to quantify the delayed effects of flows on aquatic ecosystems using 33 years of monitoring data on macroinvertebrates and water quality and 51 years of hydrological data, spanning 2,300 km of the Murray River, Australia. By incorporating the delayed effects of hydrological indices, along with physicochemical variables, into generalized additive models to form distributed lag nonlinear models for macroinvertebrate richness and abundance, we found that the effects of floods on macroinvertebrates could last up to 32 years, depending on their magnitude. The models indicated that a large flood can cause an initial depression in macroinvertebrate abundance and richness, followed by a sustained increase persisting for over 25 years before returning to preflood levels. Twelve hydrological indices representing the magnitude of flow events were evaluated, with the q90/med (the 6‐monthly flow exceeded 10% of time, divided by the long‐term median) performing (slightly) better than other indices. Competing hypotheses for the mechanisms underlying the apparent long‐term effect of floods were considered, with the most plausible explanation being the flood‐mediated influx of allochthonous organic matter, especially coarse and large woody debris, that might drive the persistent change in the aquatic community. It is generally believed that macroinvertebrate communities recover quickly after floods, but our findings suggest that we may need to reconceptualize the effects that floods can have on aquatic macroinvertebrates and the communities they support, especially in the context of flow restoration and climate change.
Comparisons Between Tethyan Anorthosite‐Bearing Ophiolites and Archean Anorthosite‐Bearing Layered Intrusions: Implications for Archean Geodynamic Processes
Authors: Sotiriou Paul , Polat Ali
Published: July 2020

Elucidating the petrogenesis and geodynamic setting(s) of anorthosites in Archean layered intrusions and Tethyan ophiolites has significant implications for crustal evolution and growth throughout Earth history. Archean anorthosite‐bearing layered intrusions occur on every continent. Tethyan ophiolites occur in Europe, Africa, and Asia. In this contribution, the field, petrographic, petrological, and geochemical characteristics of 100 Tethyan anorthosite‐bearing ophiolites and 155 Archean anorthosite‐bearing layered intrusions are compared. Tethyan anorthosite‐bearing ophiolites range from Devonian to Paleocene in age are variably composite, contain anorthosites with highly calcic (An44–100) plagioclase and magmatic amphibole. These ophiolites formed predominantly at convergent plate margins, with some forming in mid‐ocean ridge, continental rift, and mantle plume settings. The predominantly convergent plate margin tectonic setting of Tethyan anorthosite‐bearing ophiolites is indicated by negative Nb and Ti anomalies and magmatic amphibole. Archean anorthosite‐bearing layered intrusions are Eoarchean to Neoarchean in age, have megacrystic anorthosites with highly calcic (An20–100) plagioclase and magmatic amphibole, and are interlayered with gabbros and leucogabbros and intrude pillow basalts. These Archean layered intrusions are interpreted to have predominantly formed at convergent plate margins, with the remainder forming in mantle plume, continental rift, oceanic plateau, postorogenic, anorogenic, mid‐ocean ridge, and passive continental margin settings. These layered intrusions predominantly crystallized from hydrous Ca‐ and Al‐rich tholeiitic magmas. The field, petrographic, and geochemical similarities between Archean and Tethyan anorthosites indicate that they were produced by similar geodynamic processes mainly in suprasubduction zone settings. We suggest that Archean anorthosite‐bearing layered intrusions and spatially associated greenstone belts represent dismembered subduction‐related Archean ophiolites.
A Semianalytical Interface Model of Soil Freeze/Thaw and Permafrost Evolution
Authors: Devoie Élise G. , Craig James R.
Published: July 2020

A physically based one‐dimensional sharp‐interface model of active layer evolution and permafrost thaw is presented. This computationally efficient, semianalytical, nonequilibrium solution to soil freeze‐thaw problems in partially saturated media is proposed as a component of hydrological models to describe seasonal ground ice, active layer evolution, and changes in permafrost temperature and extent. The model is developed and validated against the analytical Stefan solution and a finite volume coupled heat and mass transfer model of freeze‐thaw in unsaturated porous media. Unlike analytic models, the interface model provides a nonequilibrium solution to the heat equation while permitting a wide range of temporally variable boundary conditions and supporting the simulation of multiple interfaces between frozen and unfrozen soils. The model is implemented for use in discontinuous permafrost peatlands where soil properties are highly dependent on soil ice content and infiltration capacity is high. It is demonstrated that the model is suitable for the representation of variably saturated active layer and permafrost evolution in cases both with and without a talik.
Retraction Note: LAMP2 expression dictates azacytidine response and prognosis in MDS/AML
Authors: Dubois Alix , Furstoss Nathan , Calleja Anne , Zerhouni Marwa , Cluzeau Thomas , Savy Coline , Marchetti Sandrine , Hamouda Mohamed Amine , Boulakirba Sonia , Orange François , Lacas-Gervais Sandra , Karsenti Jean-Michel , Mounier Nicolas , Tamburini Jérôme , Puissant Alexandre , Luciano Frederic , Jacquel Arnaud , Auberger Patrick , Robert Guillaume
Published: July 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Transparent inorganic multicolour displays enabled by zinc-based electrochromic devices
Authors: Zhang Wu , Li Haizeng , Yu William W. , Elezzabi Abdulhakem Y.
Published: July 2020

Electrochromic displays have been the subject of extensive research as a promising colour display technology. The current state-of-the-art inorganic multicolour electrochromic displays utilize nanocavity structures that sacrifice transparency and thus limit their diverse applications. Herein, we demonstrate a transparent inorganic multicolour display platform based on Zn-based electrochromic devices. These devices enable independent operation of top and bottom electrochromic electrodes, thus providing additional configuration flexibility of the devices through the utilization of dual electrochromic layers under the same or different colour states. Zn–sodium vanadium oxide (Zn–SVO) electrochromic displays were assembled by sandwiching Zn between two SVO electrodes, and they could be reversibly switched between multiple colours (orange, amber, yellow, brown, chartreuse and green) while preserving a high optical transparency. These Zn–SVO electrochromic displays represent the most colourful transparent inorganic-based electrochromic displays to date. In addition, the Zn–SVO electrochromic displays possess an open-circuit potential (OCP) of 1.56 V, which enables a self-colouration behaviour and compelling energy retrieval functionality. This study presents a new concept integrating high transparency and high energy efficiency for inorganic multicolour displays.
Correction: Multi-trait analysis for genome-wide association study of five psychiatric disorders
Authors: Wu Yulu , Cao Hongbao , Baranova Ancha , Huang Hailiang , Li Sheng , Cai Lei , Rao Shuquan , Dai Minhan , Xie Min , Dou Yikai , Hao Qinjian , Zhu Ling , Zhang Xiangrong , Yao Yin , Zhang Fuquan , Xu Mingqing , Wang Qiang
Published: July 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Targeted inhibition of endothelial calpain delays wound healing by reducing inflammation and angiogenesis
Authors: Yi Chenlong , Wu Weihua , Zheng Dong , Peng Guangying , Huang Haoyue , Shen Zhenya , Teng Xiaomei
Published: July 2020

Wound healing is a multistep phenomenon that relies on complex interactions between various cell types. Calpains are a well-known family of calcium-dependent cysteine proteases that regulate several processes, including cellular adhesion, proliferation, and migration, as well as inflammation and angiogenesis. CAPNS1, the common regulatory subunit of Calpain-1 and 2, is indispensable for catalytic subunit stabilization and activity. Calpain inhibition has been shown to reduce organ damage in various disease models. Here, we report that endothelial calpain-1/2 is crucially involved in skin wound healing. Using a mouse genetic model where Capns1 is deleted only in endothelial cells, we showed that calpain-1/2 disruption is associated with reduced injury-activated inflammation, reduced CD31+ blood vessel density, and delayed wound healing. Moreover, in cultured HUVECs, inhibition of calpain reduced TNF-α-induced proliferation, migration, and tube formation. Deletion of Capns1 was associated with elevated levels of IκB and downregulation of β-catenin expression in endothelial cells. These observations delineate a novel mechanistic role for calpain in the crosstalk between inflammation and angiogenesis during skin repair.
Development and validation of prognostic index based on autophagy-related genes in patient with head and neck squamous cell carcinoma
Authors: Feng Hao , Zhong Linna , Yang Xiangjun , Wan Qianbing , Pei Xibo , Wang Jian
Published: July 2020

Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers worldwide, accounting for almost 50% of all malignancies in developing nations. Autophagy plays a vital role in cancer initiation, malignant progression, and resistance to treatment. However, autophagy-related gene sets have rarely been analyzed in HNSCC. Hence, it is necessary to assess its clinical and pathological significance in a larger cohort of patients with HNSCC. The purpose of this study was to establish a novel autophagy-related prognostic marker for HNSCC. We screened 232 autophagy-related genes (ARGs) and identified 38 differentially expressed ARGs in The Cancer Genome Atlas (TCGA) cohorts. The prognosis-related ARGs signature, established using the univariate and multivariate Cox proportional regression models, consists of 10 ARGs that could divide patients into high-risk and low-risk groups. Survival analysis indicated that patients in the high-risk group had dramatically shorter overall survival compared with their low-risk counterparts. Cox regression analysis further confirmed the independent prognostic value of the autophagy-related signature, and the area under the receiver operating characteristic curve of the combined prognostic model was 0.722. Finally, the efficacy of autophagy-related signature was also validated by an independent cohort from the Gene Expression Omnibus (GEO) database. Collectively, we successfully constructed a novel autophagy-related signature for the prediction of prognosis in patients with HNSCC.
PRDM15 is a key regulator of metabolism critical to sustain B-cell lymphomagenesis
Authors: Mzoughi Slim , Fong Jia Yi , Papadopoli David , Koh Cheryl M. , Hulea Laura , Pigini Paolo , Di Tullio Federico , Andreacchio Giuseppe , Hoppe Michal Marek , Wollmann Heike , Low Diana , Caldez Matias J. , Peng Yanfen , Torre Denis , Zhao Julia N. , Uchenunu Oro , Varano Gabriele , Motofeanu Corina-Mihaela , Lakshmanan Manikandan , Teo Shun Xie , Wun Cheng Mun , Perini Giovanni , Tan Soo Yong , Ong Chee Bing , Al-Haddawi Muthafar , Rajarethinam Ravisankar , Hue Susan Swee-Shan , Lim Soon Thye , Ong Choon Kiat , Huang Dachuan , Ng Siok-Bian , Bernstein Emily , Hasson Dan , Wee Keng Boon , Kaldis Philipp , Jeyasekharan Anand , Dominguez-sola David , Topisirovic Ivan , Guccione Ernesto
Published: July 2020

PRDM (PRDI-BF1 and RIZ homology domain containing) family members are sequence-specific transcriptional regulators involved in cell identity and fate determination, often dysregulated in cancer. The PRDM15 gene is of particular interest, given its low expression in adult tissues and its overexpression in B-cell lymphomas. Despite its well characterized role in stem cell biology and during early development, the role of PRDM15 in cancer remains obscure. Herein, we demonstrate that while PRDM15 is largely dispensable for mouse adult somatic cell homeostasis in vivo, it plays a critical role in B-cell lymphomagenesis. Mechanistically, PRDM15 regulates a transcriptional program that sustains the activity of the PI3K/AKT/mTOR pathway and glycolysis in B-cell lymphomas. Abrogation of PRDM15 induces a metabolic crisis and selective death of lymphoma cells. Collectively, our data demonstrate that PRDM15 fuels the metabolic requirement of B-cell lymphomas and validate it as an attractive and previously unrecognized target in oncology.
E2F-dependent transcription determines replication capacity and S phase length
Authors: Pennycook Betheney R. , Vesela Eva , Peripolli Silvia , Singh Tanya , Barr Alexis R. , Bertoli Cosetta , de Bruin Robertus A. M.
Published: July 2020

DNA replication timing is tightly regulated during S-phase. S-phase length is determined by DNA synthesis rate, which depends on the number of active replication forks and their velocity. Here, we show that E2F-dependent transcription, through E2F6, determines the replication capacity of a cell, defined as the maximal amount of DNA a cell can synthesise per unit time during S-phase. Increasing or decreasing E2F-dependent transcription during S-phase increases or decreases replication capacity, and thereby replication rates, thus shortening or lengthening S-phase, respectively. The changes in replication rate occur mainly through changes in fork speed without affecting the number of active forks. An increase in fork speed does not induce replication stress directly, but increases DNA damage over time causing cell cycle arrest. Thus, E2F-dependent transcription determines the DNA replication capacity of a cell, which affects the replication rate, controlling the time it takes to duplicate the genome and complete S-phase.
A perceptual scaling approach to eyewitness identification
Authors: Gepshtein Sergei , Wang Yurong , He Fangchao , Diep Dinh , Albright Thomas D.
Published: July 2020

Eyewitness misidentification accounts for 70% of verified erroneous convictions. To address this alarming phenomenon, research has focused on factors that influence likelihood of correct identification, such as the manner in which a lineup is conducted. Traditional lineups rely on overt eyewitness responses that confound two covert factors: strength of recognition memory and the criterion for deciding what memory strength is sufficient for identification. Here we describe a lineup that permits estimation of memory strength independent of decision criterion. Our procedure employs powerful techniques developed in studies of perception and memory: perceptual scaling and signal detection analysis. Using these tools, we scale memory strengths elicited by lineup faces, and quantify performance of a binary classifier tasked with distinguishing perpetrator from innocent suspect. This approach reveals structure of memory inaccessible using traditional lineups and renders accurate identifications uninfluenced by decision bias. The approach furthermore yields a quantitative index of individual eyewitness performance.
HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains
Authors: Francis Ashwanth C. , Marin Mariana , Singh Parmit K. , Achuthan Vasudevan , Prellberg Mathew J. , Palermino-Rowland Kristina , Lan Shuiyun , Tedbury Philip R. , Sarafianos Stefan G. , Engelman Alan N. , Melikyan Gregory B.
Published: July 2020

The early steps of HIV-1 infection, such as uncoating, reverse transcription, nuclear import, and transport to integration sites are incompletely understood. Here, we imaged nuclear entry and transport of HIV-1 replication complexes in cell lines, primary monocyte-derived macrophages (MDMs) and CD4+ T cells. We show that viral replication complexes traffic to and accumulate within nuclear speckles and that these steps precede the completion of viral DNA synthesis. HIV-1 transport to nuclear speckles is dependent on the interaction of the capsid proteins with host cleavage and polyadenylation specificity factor 6 (CPSF6), which is also required to stabilize the association of the viral replication complexes with nuclear speckles. Importantly, integration site analyses reveal a strong preference for HIV-1 to integrate into speckle-associated genomic domains. Collectively, our results demonstrate that nuclear speckles provide an architectural basis for nuclear homing of HIV-1 replication complexes and subsequent integration into associated genomic loci.
Author Correction: Representation of molecular structures with persistent homology for machine learning applications in chemistry
Authors: Townsend Jacob , Micucci Cassie Putman , Hymel John H. , Maroulas Vasileios , Vogiatzis Konstantinos D.
Published: July 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
SARS-CoV-2 proteome microarray for global profiling of COVID-19 specific IgG and IgM responses
Authors: Jiang He-wei , Li Yang , Zhang Hai-nan , Wang Wei , Yang Xiao , Qi Huan , Li Hua , Men Dong , Zhou Jie , Tao Sheng-ce
Published: July 2020

We still know very little about how the human immune system responds to SARS-CoV-2. Here we construct a SARS-CoV-2 proteome microarray containing 18 out of the 28 predicted proteins and apply it to the characterization of the IgG and IgM antibodies responses in the sera from 29 convalescent patients. We find that all these patients had IgG and IgM antibodies that specifically bind SARS-CoV-2 proteins, particularly the N protein and S1 protein. Besides these proteins, significant antibody responses to ORF9b and NSP5 are also identified. We show that the S1 specific IgG signal positively correlates with age and the level of lactate dehydrogenase (LDH) and negatively correlates with lymphocyte percentage. Overall, this study presents a systemic view of the SARS-CoV-2 specific IgG and IgM responses and provides insights to aid the development of effective diagnostic, therapeutic and vaccination strategies.
A cohort study of medication adherence among patients with chronic obstructive pulmonary disease in Egypt
Authors: Joël Ladner  , El Badrawy Mahinour , Nofal Anas , Saba Joseph , Audureau Etienne
Published: July 2020

Chronic obstructive pulmonary disease (COPD), the most common chronic respiratory disease, is expected to become the third leading cause of death worldwide in 2020. A prospective cohort study conducted in 2017 and 2018 aimed to identify factors associated with inhaler treatment adherence in patients with COPD in Cairo (Egypt). Physicians collected data regarding patient deaths, treatment-related adverse events, and patients’ social support (no support, patient, support by spouse, children, and siblings) from their patients with COPD. The reason for treatment discontinuation was categorized as per patient decision or per physician decision. Adherence was categorized as treatment continued or treatment stopped. Patients who decided to stop treatment were considered non-adherent to COPD therapy. A total of 1311 patients as well as 98 physicians and 205 pharmacists were included. Pharmacists and social support (spouse, children/siblings) were identified as key positive factors in patients’ decisions to adhere to their prescribed COPD treatment regimens. A total of 631 patients (48.1%) stopped the treatment, including 170 (27.0%) due to patient decision and 55 (8.7%) deceased. After Cox model analysis, a low number of patients (6–19) attended by the pharmacist was a significant predictive factor (hazard ratio [HR] = 1.40, 95% confidence interval [CI] = 1.03–1.91, p = 0.03) for deciding to stop treatment. A wife or husband (HR = 0.85, 95% CI = 0.72–1.02, p = 0.07) as well as children or brother/sister (HR = 0.77, 95% CI = 0.57–1.04, p = 0.08) provided a positive effect for continued treatment. Pharmacists are well positioned to play a role as an essential public health resource that can help improve adherence as well as social support that should be considered as an important component to improve adherence to long-term therapy in COPD as well as other chronic non-communicable diseases in low- and middle-income countries.
Linear response of a superfluid Fermi gas inside its pair-breaking continuum
Authors: Kurkjian H. , Tempere J. , Klimin S. N.
Published: July 2020

We study the signatures of the collective modes of a superfluid Fermi gas in its linear response functions for the order-parameter and density fluctuations in the Random Phase Approximation (RPA). We show that a resonance associated to the Popov-Andrianov (or sometimes “Higgs”) mode is visible inside the pair-breaking continuum at all values of the wavevector q, not only in the (order-parameter) modulus-modulus response function but also in the modulus-density and density-density responses. At nonzero temperature, the resonance survives in the presence of thermally broken pairs even until the vicinity of the critical temperature Tc, and coexists with both the Anderson-Bogoliubov modes at temperatures comparable to the gap Δ and with the low-velocity phononic mode predicted by RPA near Tc. The existence of a Popov-Andrianov-“Higgs” resonance is thus a robust, generic feature of the high-energy phenomenology of pair-condensed Fermi gases, and should be accessible to state-of-the-art cold atom experiments.
Axial loading and posture cues in contraction of transversus abdominis and multifidus with exercise
Authors: Owen Patrick J. , Rantalainen Timo , Scheuring Richard A. , Belavy Daniel L.
Published: July 2020

Astronauts are at increased risk of spine injury. With a view to developing training approaches for the muscles of the spine in microgravity, this study examined the effects of axial loading and postural cues on the contraction of transversus abdominis and lumbar multifidus in supine lying using a novel exercise device (GravityFit). Thirty (18 males and 12 females) endurance-trained runners without a history of spinal pain aged 33–55 years were recruited. Magnetic resonance imaging (MRI) was performed under one rest and five exercise conditions, which involved variations in axial loading and postural cues. Whole volume of the abdominal and lumbar paraspinal muscles was imaged and transversus abdominis thickness and length and multifidus anteroposterior and mediolateral thickness measured. Transversus abdominis contraction was greatest in the ‘stretch tall plus arm extension’ (length, − 15%, P < 0.001; thickness, + 19%, P < 0.001) and ‘stretch tall plus arm extension and thoracic cue’ (length, − 16%, P < 0.001; thickness, + 18%, P < 0.001) conditions. The contraction of multifidus was the greatest in the ‘arm extension and thoracic cue’ (anteroposterior, + 3.0%, P = 0.001; mediolateral, − 4.2%, P < 0.001) and ‘stretch tall plus arm extension and thoracic cue’ (anteroposterior, + 6.0%, P < 0.001; mediolateral, − 2.1%, P = 0.022) conditions. This study provides proof-of-principle for an exercise approach that may be used to facilitate the automatically contraction of the transversus abdominis and multifidus muscles. Axial loading of the body, with or without arm loading, most consistently led to contraction of the transversus abdominis and lumbar multifidus muscles, and regional differences existed in the contraction within the muscles.
Acute stress improves the effectivity of cognitive emotion regulation in men
Authors: Langer Katja , Hagedorn Bianca , Stock Lisa-Marie , Otto Tobias , Wolf Oliver T. , Jentsch Valerie L.
Published: July 2020

Emotion regulation is crucial for coping with stressors but in turn can also be influenced by stress. Initial studies provided mixed evidence showing either beneficial or impairing stress effects on cognitive emotion regulation depending on stress timing, sex or the regulatory strategy. Here, we investigated the impact of acute stress on different emotion regulation strategies in men and women. N = 118 healthy participants were subjected to the Trier Social Stress Test or a control condition after which they completed an emotion regulation paradigm, requiring them to regulate their emotions in response to negative pictures using reappraisal or distraction. Cortisol levels were repeatedly measured to quantify changes in HPA axis activity. Affective ratings and pupil dilation served to measure emotion regulation success and the cognitive effort to regulate emotions. Stress reduced arousal and increased valence and success ratings for reappraisal in men, whereas no significant stress effects were found in women. Moreover, stressed men displayed a significant expansion of pupil diameter during reappraisal suggesting enhanced cognitive regulatory engagement, which ultimately may have led to better emotion regulation outcomes. Cortisol secretion positively correlated with subjective reappraisal success in men, suggesting a glucocorticoid-driven mechanism that may promote emotion regulatory performance in the aftermath of stress.
Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure
Authors: Kelly Sandra M. , Lanigan Noreen , O’Neill Ian J. , Bottacini Francesca , Lugli Gabriele Andrea , Viappiani Alice , Turroni Francesca , Ventura Marco , van Sinderen Douwe
Published: July 2020

In the current study, we show that biofilm formation by various strains and species belonging to Bifidobacterium, a genus that includes gut commensals with reported health-promoting activities, is induced by high concentrations of bile (0.5% (w/v) or higher) and individual bile salts (20 mM or higher), rather than by acid or osmotic stress. The transcriptomic response of a bifidobacterial prototype Bifidobacterium breve UCC2003 to such high bile concentrations was investigated and a random transposon bank of B. breve UCC2003 was screened for mutants that affect biofilm formation in order to identify genes involved in this adaptive process. Eleven mutants affected in their ability to form a biofilm were identified, while biofilm formation capacity of an insertional mutation in luxS and an exopolysaccharide (EPS) negative B. breve UCC2003 was also studied. Reduced capacity to form biofilm also caused reduced viability when exposed to porcine bile. We propose that bifidobacterial biofilm formation is an adaptive response to high concentrations of bile in order to avoid bactericidal effects of high bile concentrations in the gastrointestinal environment. Biofilm formation appears to be a multi-factorial process involving EPS production, proteins and extracellular DNA release, representing a crucial strategy in response to bile stress in order to enhance fitness in the gut environment.