bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Regional Ventilation During Recruitment Maneuver by Electrical Impedance Tomography in Dogs
Authors: Ambrósio Aline Magalhães , Sanchez Ana Flávia , Pereira Marco Aurélio Amador , Andrade Felipe Silveira Rego Monteiro De , Rodrigues Renata Ramos , Vitorasso Renato de Lima , Moriya Henrique Takachi , Fantoni Denise Tabacchi
Published: February 2022

Background

During protective mechanical ventilation, electrical impedance tomography (EIT) is used to monitor alveolar recruitment maneuvers as well as the distribution of regional ventilation. This technique can infer atelectasis and lung overdistention during mechanical ventilation in anesthetized patients or in the ICU. Changes in lung tissue stretching are evaluated by monitoring the electrical impedance of lung tissue with each respiratory cycle.

Objective

This study aimed to evaluate the distribution of regional ventilation during recruitment maneuvers based on the variables obtained in pulmonary electrical impedance tomography during protective mechanical ventilation, focusing on better lung recruitment associated with less or no overdistention.

Methods

Prospective clinical study using seven adult client–owned healthy dogs, weighing 25 ± 6 kg, undergoing elective ovariohysterectomy or orchiectomy. The animals were anesthetized and ventilated in volume-controlled mode (7 ml.kg−1) with stepwise PEEP increases from 0 to 20 cmH2O in steps of 5 cmH2O every 5 min and then a stepwise decrease. EIT, respiratory mechanics, oxygenation, and hemodynamic variables were recorded for each PEEP step.

Results

The results show that the regional compliance of the dependent lung significantly increased in the PEEP 10 cmH2O decrease step when compared with baseline (p < 0.027), and for the nondependent lung, there was a decrease in compliance at PEEP 20 cmH2O (p = 0.039) compared with baseline. A higher level of PEEP was associated with a significant increase in silent space of the nondependent regions from the PEEP 10 cmH2O increase step (p = 0.048) until the PEEP 15 cmH2O (0.019) decrease step with the highest values at PEEP 20 cmH20 (p = 0.016), returning to baseline values thereafter. Silent space of the dependent regions did not show any significant changes. Drive pressure decreased significantly in the PEEP 10 and 5 cmH2O decrease steps (p = 0.032) accompanied by increased respiratory static compliance in the same PEEP step (p = 0.035 and 0.018, respectively).

Conclusions

The regional ventilation distribution assessed by EIT showed that the best PEEP value for recruitment maintenance, capable of decreasing areas of pulmonary atelectasis in dependent regions promoting less overinflation in nondependent areas, was from 10 to 5 cmH2O decreased steps.
Chlorogenic Acid Attenuates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
Authors: Chen Jiali , Chen Daiwen , Yu Bing , Luo Yuheng , Zheng Ping , Mao Xiangbing , Yu Jie , Luo Junqiu , Huang Zhiqing , Yan Hui , He Jun
Published: February 2022

Chlorogenic acid (CGA) is a natural polyphenol that possesses potent antioxidant activity. However, little is known about its exact role in regulating the intestinal health under oxidative stress. This study was conducted to explore the effect of dietary CGA supplementation on intestinal barrier functions in weaned pigs upon oxidative stress. Twenty-four weaned pigs were allocated to three treatments and were given a basal diet (control) or basal diet containing CGA (1,000 mg/kg) for 21 days. Pigs were challenged by sterile saline (control) or diquat [10 mg/kg body weight (BW)] on the 15th day. Results showed that CGA attenuated the BW reduction, reduced the serum concentrations of diamine oxidase and D-lactate, and elevated serum antioxidant enzymes activities in diquat-challenged weaned pigs (P < 0.05). Moreover, diquat challenge decreased villus height and activities of sucrase and alkaline phosphatase in jejunum and ileum (P < 0.05), but CGA elevated the villus height and enzyme activities in the intestinal mucosa (P < 0.05). In addition, CGA not only decreased the expression levels of Bax, caspase-3, and caspase-9 (P < 0.05) but also elevated the expression levels of sodium glucose transport protein-1, glucose transporter-2, occludin, claudin-1, zonula occludens-1, and antioxidant genes such as nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1 in intestinal mucosa of weaned pigs upon oxidative stress (P < 0.05). These findings suggested that CGA can attenuate oxidative stress-induced growth retardation and intestinal mucosa disruption, which was linked to elevated antioxidative capacity and enhanced intestinal barrier integrity.
Influence of the Dynamic Effects and Grasping Location on the Performance of an Adaptive Vacuum Gripper
Authors: Maggi Matteo , Mantriota Giacomo , Reina Giulio
Published: February 2022

A rigid in-plane matrix of suction cups is widely used in robotic end-effectors to grasp objects with flat surfaces. However, this grasping strategy fails with objects having different geometry e.g., spherical and cylindrical. Articulated rigid grippers equipped with suction cups are an underinvestigated solution to extend the ability of vacuum grippers to grasp heavy objects with various shapes. This paper extends previous work by the authors in the development of a novel underactuated vacuum gripper named Polypus by analyzing the impact of dynamic effects and grasping location on the vacuum force required during a manipulation cycle. An articulated gripper with suction cups, such as Polypus, can grasp objects by adhering to two adjacent faces, resulting in a decrease of the required suction action. Moreover, in the case of irregular objects, many possible grasping locations exist. The model explained in this work contributes to the choice of the most convenient grasping location that ensures the minimum vacuum force required to manipulate the object. Results obtained from an extensive set of simulations are included to support the validity of the proposed analytical approach.
Emotional Dissonance, Job Stress, and Intrinsic Motivation of Married Women Working in Call Centers: The Roles of Work Overload and Work-Family Conflict
Authors: Jeon Moo-Kyeong , Yoon HyunJoong , Yang Yuha
Published: February 2022

This research was designed to test and extend the model of emotional dissonance. Previous models of emotional dissonance, such as the Job Demand-Resource (JD-R) and the Stress-Strain-Outcome (SSO) models, are limited in that they do not account for the influences of work and work–family-related conflicts. The present paper focused on emotional labor carried out by married women working in call centers. We developed the model of emotional dissonance influencing intrinsic motivation and job stress, with the moderating effects of work overload and work–family conflict. The data of 468 employees analyzed using least square regression showed that that emotional dissonance is positively related to job stress, but is negatively related to intrinsic motivation. Both work overload and work–family conflict were found to be significant moderators that aggravate the positive relationships between emotional dissonance and job stress, and the negative relationships between emotional dissonance and intrinsic motivation. Theoretical and practical implications on emotional labor and emotional dissonance are discussed.
Neural Network Prediction for Ice Shapes on Airfoils Using iceFoam Simulations
Authors: Strijhak Sergei , Ryazanov Daniil , Koshelev Konstantin , Ivanov Aleksandr
Published: February 2022

In this article the procedure and method for the ice accretion prediction for different airfoils using artificial neural networks (ANNs) are discussed. A dataset for the neural network is based on the numerical experiment results—obtained through iceFoam solver—with four airfoils (NACA0012, General Aviation, Business Jet, and Commercial Transport). Input data for neural networks include airfoil and ice geometries, transformed into a set of parameters using a parabolic coordinate system and Fourier series expansion. Besides input features include physical parameters of flow (velocity, temperature, droplets diameter, liquid water content, time of ice accretion) and angle of attack. The novelty of this work is in that the neural network dataset includes various airfoils and the data augmentation technique being a combination of all time slices. Several artificial neural networks (ANNs), fully connected networks (FCNNs), and convolutional networks (CNNs) were trained to predict airfoil ice shapes. Two different loss functions were considered. In order to improve performance of models, batch normalization and dropout layers were used. The most accurate results of ice shape prediction were obtained using CNN and FCNN that applied batch normalization and dropout layers to output neurons of each layer.
Mission Test Campaign for the EIRSAT-1 Engineering Qualification Model
Authors: Doyle Maeve , Dunwoody Rachel , Finneran Gabriel , Murphy David , Reilly Jack , Thompson Joseph , Reddy Akarapu Sai Krishna , Mangan Joseph , Walsh Sarah , Erkal Jessica , Marshall Fergal , Salmon Lána , Somers Eoghan , Ha Lily , Palma David , Martin-Carrillo Antonio , McBreen Sheila , McKeown David , O’Connor William , Shortt Brian , Uliyanov Alexey , Wall Ronan , Hanlon Lorraine
Published: February 2022

The compact, standardised form factor of CubeSats allows for the use of commercial off-the-shelf components, reducing traditional barriers to entry, such as cost and development time. More than 1500 of these small spacecraft have been launched in the past 20 years, with improving capabilities that enable a wide range of mission profiles. The Educational Irish Research Satellite, EIRSAT-1, is a CubeSat being developed by a student-led team with goals that span education, technology demonstration and science. A comprehensive mission test plan, in which in-flight conditions are simulated, has been developed for EIRSAT-1 and implemented using an engineering qualification model of the spacecraft. In addition to verifying 41 mission requirements, the successful execution of the mission test plan established that the full satellite system can perform the intended mission. Mission testing also proved to be an invaluable tool to prepare for launch and operations, providing the team with a more complete understanding of the satellite’s expected on-orbit behaviour. This work presents a detailed description of the mission test planning process and implementation, as well as key results and lessons learned. In doing so, this work aims to improve the on-orbit reliability of CubeSats by disseminating resources and good practice around mission testing.
Efficacy of Bacillus subtilis XZ18-3 as a Biocontrol Agent against Rhizoctonia cerealis on Wheat
Authors: Yi Yanjie , Luan Pengyu , Liu Shifei , Shan Youtian , Hou Zhipeng , Zhao Shuyun , Jia Shao , Li Ruifang
Published: February 2022

Rhizoctonia cerealis is a major fungal pathogen of wheat that causes great yield losses in all wheat-growing regions of the world. The biocontrol agent Bacillus subtilis XZ18-3 was investigated for inhibiting R. cerealis growth in wheat. The results of the mycelial growth test showed that the sterile filtrate of B. subtilis XZ18-3 could significantly inhibit the mycelial growth of R. cerealis and cause swelling and rupture of the mycelium. Observation by transmission electron microscopy indicated that the sterile filtrate could penetrate the cellular membrane of Rhizoctoniacerealis, resulting in organelle destruction. The effect of the sterile filtrates on the pathogen cells, shown through fluorescent microscopy using different stains, revealed the mechanism by which the sterile filtrate caused DNA fragmentation, accumulation of ROS and changes in cell membrane permeability. To reach a better treatment of the soil-borne fungi, the components of a wettable powder were screened and an optimised formula determined (30.0% kaolin, 4.0% polyvinyl alcohol, 8.0% Tween-80, 2.0% polyethylene glycol and 100% fermentation broth). A quality index analysis revealed that the wetting powder reached acceptable biological pesticide standards. Pot control experiments showed that the wettable powder of B. subtilis XZ18-3 effectively controlled the pathogens with an efficacy of 88.28%. This study has provided the potential biocontrol agents (BCAs) for wheat sharp eyespot disease.
Microclimate and Plant Transpiration of Tomato (Solanum lycopersicum L.) in a Sunken Solar Greenhouse in North China
Authors: Yang Li , Liu Haijun , Cohen Shabtai , Gao Zhuangzhuang
Published: February 2022

The solar greenhouse is a common protected structure for crop production when ambient temperatures are low. In the North China Plain (NCP) winter temperatures are very low and an improved solar greenhouse with a lowered soil surface (0.5–1.5 m deep), referred to as a sunken solar greenhouse (SSG), is used. A four-season experiment was conducted in a commercial SSG with tomato crops to characterize internal microclimate, sap flow (SF) and crop coefficients. Results show that temperature inside the SSG could be more than 20 °C higher than outside in winter, which favors tomato growth and resulted in acceptable yields. Daily total SF was related to solar radiation, vapor pressure deficit (VPD) and temperature, in that order, both in winter and summer. The decoupling coefficient (which is the ratio of radiative to aerodynamic influences on evapotranspiration) in daytime was 0.76 in winter and 0.84 in summer, indicating strong decoupling (i.e., predominance of radiative influences) of the internal environment where wind speed was low. Basal crop coefficients at the mid stage of crop growth averaged 1.15–1.43 in winter and 0.91–0.92 in spring and summer. Thus, in the SSG, for similar climatic conditions sap flow in winter was higher than that in summer, which should be considered in irrigation scheduling.
Impact of Market Participation on Household Food Security among Smallholder Irrigators in KwaZulu-Natal, South Africa
Authors: Cele Thobani , Mudhara Maxwell
Published: February 2022

Food insecurity is a global challenge. The reduction of hunger is a Sustainable Development Goals (SDG) target widely seen as a measure for evaluating the progress of the well-being of rural people. This study aimed to investigate the impact of collective action and market participation on household food security. Data were collected using a structured survey questionnaire from 243 randomly selected households in Msinga Local Municipality. The binary probit model findings revealed that farmers’ group membership, market participation, gender, the total number of cattle owned, farm income, and credit access increased the likelihood of a food-secure household. This paper’s findings have crucial implications for the government and other development agencies for improving household food-security status. The findings identified collective action and market participation to be contributing to food-insecurity alleviation. The government and private organisations need to encourage and empower existing farmers’ groups through community mobilisation.
Assessment of Soil Redistribution Following Land Rehabilitation with an Apple Orchard in Hilly Regions of Central Iran
Authors: Ayoubi Shamsollah , Mohammadi Ameneh , Abdi Mohammad Reza , Abbaszadeh Afshar Farideh , Wang Lin , Zeraatpisheh Mojtaba
Published: February 2022

This study was executed to explore soil redistribution and soil quality changes induced by land degradation and then rehabilitation by orchard plantation in different slope positions in a semi-arid region in central Iran. A total of 72 surface soil samples (0–30 cm) were collected from three land uses (natural rangelands, dryland farming, and apple orchards) in four slope positions (shoulder, backslope, footslope, and toeslope). The soil physicochemical properties and magnetic parameters were measured, and soil redistribution was determined in the selected soil samples using the 137Cs technique. The results showed that rangeland degradation and, subsequently, rainfed cultivation, led to a significant decline in the soil quality indicators, such as soil organic matter (SOM), total nitrogen (TN), available potassium (Kava), and available phosphorous (Pava), thus incurring further soil loss, as determined by the 137Cs technique. Conversely, the conversion and rehabilitation of drylands to apple orchards cultivated on the contour terraces improved soil quality significantly and decreased soil loss (p < 0.05) and soil quality grade (p < 0.01). Additionally, the findings indicated that slope positions relative to land use change had a reasonable impact on the variability of soil properties and soil loss and deposition. The results of 137Cs analysis showed that the drylands had the highest soil loss (185.3 t ha−1 yr−1) and maximum sedimentation (182. 5 t ha−1 yr−1) in the shoulder and footslope positions, respectively. The random forest model applied between 137Cs inventory and soil properties indicated that calcium carbonate equivalent (CCE), TN, Pava, Kava, and bulk density (ρb) could explain 75% of the total variability in 137Cs inventory with high R2 (0.94) and low RMSE (111.29). Magnetic measurements have shown great potential as a cost-effective and fast method for assessing soil redistribution in hilly regions, as confirmed by the findings of the 137Cs analysis, which agreed well with the magnetic susceptibility at low frequency (χlf). Overall, the results confirmed that restoring abandoned dryland by orchard cultivation may improve soil quality and diminish soil loss in the semi-arid region of Iran. However, further research is required to assess other aspects of the ecosystem affected by this restoration.
Glyphosate Excessive Use Affects Citrus Growth and Yield: The Vicious (and Unsustainable) Circle in Brazilian Orchards
Authors: Martinelli Rodrigo , Rufino Luiz Renato , Alcántara-de la Cruz Ricardo , da Conceição Patricia Marluci , Monquero Patricia Andrea , de Azevedo Fernando Alves
Published: February 2022

The excessive use of glyphosate by Brazilian citrus growers leads to a vicious and unsustainable circle: Increasing the glyphosate use and the selection pressure of resistant/tolerant weeds, as well as the phytointoxication of the crop. In addition, there is speculation on the consequences of using glyphosate and the studies are not conclusive. Therefore, this study aimed to evaluate the glyphosate management in citrus orchards by assessing its effects in a 5-year field experiment using different doses and application frequencies. Here, we determine the weed control levels, the orchard growth and fruit yield, as well as the economic viability of the treatments. Higher weed control was observed more often with the increasing frequency of glyphosate application, and occasionally with increasing doses. However, some species predominated even at high glyphosate usage, such as BIDPI (Bidens pilosa (L.)), RAPRA (Raphanus raphanistrum (L.)), and ERICA (Conyza canadensis (L.) Cronquist). Phytotoxicity symptoms were demonstrated up to the fourth year of the orchard, and onwards the plants no longer expressed them. This was a highlight, since there was a decrease over time in growth (up to 5.3 m3) and fruit yield (up to 36.3 t ha−1), with losses that reached −56% of the total income. This is the first report to demonstrate that the increase in glyphosate usage can occasionally increase weed control, but it can also decrease orchard development and its financial viability.
Wax Extracted from Waste Cauliflower Leaves Shows Potential Antitranspirant Efficacy When Applied to Rapeseed Plants
Authors: Faralli Michele , Weerasinghe Minuka , Leung Gee-Sian , Marriott Ray , Miles Melville , Kettlewell Peter
Published: February 2022

The stress from excessive transpiration of water from plant leaves can damage crop growth during droughts. Specific commercial biostimulant products (antitranspirants) are available to reduce transpiration when applied to plants, but these products are expensive. Antitranspirants from waste would be cheaper, and the aim of this research was to extract leaf wax from a fresh produce processing waste and conduct a preliminary evaluation of its antitranspirant efficacy. Supercritical CO2 was used to extract wax from cauliflower leaves, and after formulating for spraying, the wax was applied to young rapeseed plants in three consecutive experiments. The wax was compared with a commercially-available terpene antitranspirant (di-1-p-menthene) for efficacy in reducing stomatal conductance and water use. In two of the three experiments, stomatal conductance was significantly reduced to similar extents by wax and by di-1-p-menthene, despite the wax being formulated and applied at a much lower concentration. Water use was reduced by wax in one experiment. Further research is needed on formulation, spraying conditions, and dose-response. These preliminary results demonstrate the potential for extracted leaf wax to act as a biostimulant and ameliorate plant drought stress.
The Effect of an Engineered Biostimulant Derived from Ascophyllum nodosum on Grass Yield under a Reduced Nitrogen Regime in an Agronomic Setting
Authors: Quille Patrick , Claffey Aisling , Feeney Ewan , Kacprzyk Joanna , Ng Carl K.-Y. , O’Connell Shane
Published: February 2022

Nitrogen (N) is the most important macronutrient used in modern agricultural systems to enhance crop yields; however, a significant amount of applied N is not taken up by the crop and is lost to the environment. Improving the nitrogen use efficiency (NUE) of crops can curb these environmental losses while concurrently delivering economic gains. Plant biostimulants have potential to improve NUE in agronomic settings. In this research, a granular N-containing fertilizer coated with the biostimulant PSI-362, an extract from the brown seaweed Ascophyllum nodosum, was applied to grass managed under different production systems to assess its impact on NUE. The role of soil type, pH, phosphorus (P) and potassium (K) on the efficacy of the biostimulant in improving NUE was assessed using lysimeters filled with six different soils. A significant increase in grass yield (29%) was found with PSI-362 addition at a 75% N rate over the 75% N control (8478 kg of dry matter (DM) ha−1 vs. 6772 kg of DM ha−1) over two years of trials under a simulated grazing platform of six rotations. The NUE increased to 96.6% for the PSI-362-treated grass compared to 82.8% for controls. Field-based evaluations demonstrated no decrease in yield and quality from harvested and grazed grass treated with the biostimulant when the N rate was reduced by 20 to 25%. Based on these results, the application of PSI-362 allows a reduction in nitrogen input by up to 25% without losses of grass yield or quality.
An Evaluation of Irradiation Treatment to Disinfect Soil Tare from Globodera spp.
Authors: Berger Beatrice , Schumann Lisa , Daub Matthias , König Stephan
Published: February 2022

As a severe danger to potato plants, potato cyst nematodes (PCNs) are regulated quarantine pests in many countries. Adhering soils to potato or other tuber crops provoke the uncontrolled spread of the pest after their industrial processing. The disinfection of soil tares to inactivate PCNs is therefore compulsory. In the present study, we test whether the viability and formation of PCN cysts are suppressed after treatment with γ and β irradiation. In a first experiment, nematode cysts were treated with γ or β irradiation without soil matrix to determine the minimum dose parameters in a range between 0–12 kGy. Subsequently, two representative soils that included cysts were irradiated. Irradiation effects on PCN viability and the formation of new cysts were evaluated via hatching tests and bioassays with susceptible potato plants, respectively. In addition, maize seedling growth was tested in irradiated soil tares. We found that next to γ also, β irradiation suppressed the hatch of PCN juveniles and its formation of cysts when placed in soil tares. The growth of maize seedlings was not impaired in irradiated soils compared to control soils. Both γ and β irradiation treatment offer an organism group selective and effective phytosanitary measures to disinfect soil tares from PCNs.
Jerusalem Artichoke as a Strategic Crop for Solving Food Problems
Authors: Manokhina Alexandra Anatolyevna , Dorokhov Alexey Semyonovich , Kobozeva Tamara Petrovna , Fomina Tatiana Nikolaevna , Starovoitov Viktor Ivanovich
Published: February 2022

Jerusalem artichoke is a staple for the raw material base of healthy food, beverages, feed, medicine, and fuel, in addition to being the source of its herbage and tubers. The choice of conditions for cultivating a variety depends on the purpose or direction for the crop and the products obtained from it. This research involved laboratory and on-farm studies of 16 Jerusalem artichoke varieties of different maturity groups cultivated on sod-podzolic sandy loam soils to determine their suitability for further use in feed production. The list of varieties in question is presented in this work. The gross yield of tubers obtained from varieties with early leaf wilting was 33.1–51.1 t/ha, whereas that from varieties with late leaf wilting was 14.4–43.9 t/ha. The total yield of Jerusalem artichoke raw biomass was 36.8–98.1 t/ha. Moreover, early varieties had a dominant mass of tubers in the total feed value structure (68%), whereas herbage mass (59%) prevailed in the total feed value structure of varieties with late leaf wilting. The highest total solids in tubers (25.0–26.4%) were found in varieties Korenevskiy, Novost VIRa, and Blank Brekos; whereas high inulin content was found in varieties Dieticheskiy, Kaluzhskiy, Korenevskiy, Nakhodka, Novost VIRa, and Blank Brekos. The aggregate feed value amounted to 37.0–103.4 thousand MJ/ha of metabolized energy; this exceeded energy production costs on properly selected varieties by two- to three-fold. The data obtained can be useful for growers in the introduction and evaluation of Jerusalem artichoke varieties to be cultivated for further usage and processing, including fodder purposes.
Selection and Characterization of a Bacillus Strain for Potential Application in Industrial Production of White Button Mushroom (Agaricus bisporus)
Authors: Büchner Rita , Vörös Mónika , Allaga Henrietta , Varga András , Bartal Attila , Szekeres András , Varga Sarolta , Bajzát Judit , Bakos-Barczi Nóra , Misz András , Csutorás Csaba , Hatvani Lóránt , Vágvölgyi Csaba , Kredics László
Published: February 2022

White button mushroom—Agaricus bisporus (J.E.Lange) Imbach—is among the most popular cultivated mushrooms worldwide. The most serious challenge in industrial mushroom production is the green mold disease caused by Trichoderma species. Our aim was to isolate and examine bacterial strains from mushroom casing material for their potential use as biocontrol agents. Twenty-seven bacterial strains were isolated and tested against mold pathogens of white button mushroom. The Bacillus velezensis strain SZMC 25431 was selected for further examination and tested under simulated Agaricus cultivation conditions against T. aggressivum SZMC 23834 in a 1200-L Fitotron SGC120 standard plant growth chamber. Our results showed that the bacterial treatment was effective against the pathogen in all cases, but the best results were achieved at an application concentration of 105 cells mL−1. Industrial-scale experiments were also carried out in Agaricus growing houses with a bearing surface of 480 m2: the bacterial suspension was mixed in water tanks applied for daily irrigation. The results suggest that the bacterial treatment may even increase the crop yield of A. bisporus. Based on our results, we concluded that the selected B. velezensis strain may potentially be used for biological and integrated treatment in Agaricus cultivation.
Race-Level Reporting of Incidents during Two Seasons (2015/16 to 2016/17) of Harness Racing in New Zealand
Authors: Gibson Michaela , Roca Fraga Fernando , Bolwell Charlotte , Gee Erica , Rogers Chris
Published: February 2022

The objective of this study was to describe the incident and non-incident reporting of harness racing in New Zealand, the primary injury and reporting outcomes, and to examine horse- and race-level variables associated with the odds of these outcomes. Retrospective stipendiary stewards’ reports of race day events during the 2015/16 to 2016/17 racing seasons were examined. The number of incident and non-incident events and binomial exact 95% confidence intervals (CI) were calculated per 1000 horse starts. Most reports were for non-incidents and an examination was requested for poor performance (11.06 per 1000 starts (95% CI = 10.23–11.89). Races with more than eight participants were 1.9 (95% CI = 1.13–3.4) times more likely to have an incident than races with eight or less participants. The low incidence of significant injuries such as fractures (0.13 per 1000 starts (95% CI = 0.03–0.23) reflects the lower risk of injury in harness racing compared to Thoroughbred racing. The high incidence of poor performance reports highlights the steward’s role in maintaining animal welfare to a high standard.
Aeromonas spp. Prevalence, Virulence, and Antimicrobial Resistance in an Ex Situ Program for Threatened Freshwater Fish—A Pilot Study with Protective Measures
Authors: Grilo Miguel L. , Amaro Guadalupe , Chambel Lélia , Marques Carolina S. , Marques Tiago A. , Gil Fátima , Sousa-Santos Carla , Robalo Joana I. , Oliveira Manuela
Published: February 2022

Ex situ breeding programs are important conservation tools for endangered freshwater fish. However, developing husbandry techniques that decrease the likelihood of disease, antimicrobial resistance, and virulence determinants acquisition during this process is challenging. In this pilot study, we conducted a captivity experiment with Portuguese nase (Iberochondrostoma lusitanicum), a critically endangered leuciscid species, to investigate the influence of simple protective measures (i.e., material disinfection protocols and animal handling with gloves) on the dynamics of a potential pathogenic genus, Aeromonas, as well as its virulence profiles and antimicrobial resistance signatures. Our findings show that antimicrobial resistance in Aeromonas spp. collected from I. lusitanicum significantly increased during the extent of the assay (5 weeks), with all isolates collected at the end of the study classified as multidrug-resistant. Additionally, humans handling fishes without protective measures were colonized by Aeromonas spp. The use of protective measures suggested a decreasing trend in Aeromonas spp. prevalence in I. lusitanicum, while bacterial isolates displayed significantly lower virulence index values when virulence phenotypical expression was tested at 22 °C. Despite this study representing an initial trial, which needs support from further research, protective measures tested are considered a simple tool to be applied in ex situ breeding programs for aquatic animals worldwide. Furthermore, current results raise concern regarding antimicrobial resistance amplification and zoonotic transmission of Aeromonas spp. in aquatic ex situ programs.
Domain-Aware Neural Architecture Search for Classifying Animals in Camera Trap Images
Authors: Jia Liang , Tian Ye , Zhang Junguo
Published: February 2022

Camera traps provide a feasible way for ecological researchers to observe wildlife, and they often produce millions of images of diverse species requiring classification. This classification can be automated via edge devices installed with convolutional neural networks, but networks may need to be customized per device because edge devices are highly heterogeneous and resource-limited. This can be addressed by a neural architecture search capable of automatically designing networks. However, search methods are usually developed based on benchmark datasets differing widely from camera trap images in many aspects including data distributions and aspect ratios. Therefore, we designed a novel search method conducted directly on camera trap images with lowered resolutions and maintained aspect ratios; the search is guided by a loss function whose hyper parameter is theoretically derived for finding lightweight networks. The search was applied to two datasets and led to lightweight networks tested on an edge device named NVIDIA Jetson X2. The resulting accuracies were competitive in comparison. Conclusively, researchers without knowledge of designing networks can obtain networks optimized for edge devices and thus establish or expand surveillance areas in a cost-effective way.
Development of a Composite Pain Scale in Foals: A Pilot Study
Authors: Lanci Aliai , Benedetti Beatrice , Freccero Francesca , Castagnetti Carolina , Mariella Jole , van Loon Johannes P. A. M. , Padalino Barbara
Published: February 2022

Prompt pain management is crucial in horses; however, tools to assess pain are limited. This study aimed to develop and pilot a composite scale for pain estimation in foals. The “Foal Composite Pain Scale” (FCPS) was developed based on literature and authors’ expertise. The FCPS consisted of 11 facial expressions, 4 behavioural items, and 5 physical items. Thirty-five pain-free foals (Control Group) and 15 foals experiencing pain (Pain Group) were used. Foals were video-recorded at different time points: the Control Group only at inclusion (C), while the Pain Group at inclusion (T1), after an analgesic treatment (T2), and at recovery (T3). Physical items were also recorded at the same time points. Videos were scored twice by five trained observers, blinded to group and time points, to calculate inter- and intra-observer reliability of each scale item. Fleiss’ kappa values ranged from moderate to almost perfect for the majority of the items, while the intraclass correlation coefficient was excellent (ICC = 0.923). The consistency of FCPS was also excellent (Cronbach’s alpha = 0.842). A cut-off ≥ 7 indicated the presence of pain. The Pain Group scores were significantly higher (p < 0.001) than the Control Group and decreased over time (T1, T2 > T3; p = 0.001). Overall, FCPS seems clinically applicable to quantify pain and improve the judgment of the quality of life in foals, but it needs modifications based on these preliminary findings. Consequently, further studies on a larger sample size are needed to test the feasibility and validity of the refined FCPS.
Preliminary Behavioural Observations of Horseback Safaris: Initial Insights into the Welfare Implications for Horses and Herbivorous Plains Game Species
Authors: Hodgson Evelyn , Rooney Nicola J. , Hockenhull Jo
Published: February 2022

In Africa, wildlife-watching experiences create substantial revenue from tourists that can finance wildlife conservation. Horseback safaris, where an experienced guide takes guests through the bush on horseback to observe plains game species, are a popular activity. Close encounters between ridden horses and game species are unnatural and potentially stressful situations, and horseback safaris may have adverse impacts on both the horses and the wildlife they have come to observe. This study aims to provide a preliminary insight into the behavioural responses of horses and herbivorous plains game species, including giraffe, zebra and impala, as a proxy measure of the potential welfare implications of horseback safaris. Seventeen group safari rides were observed encompassing 72 encounters with plains game species. Game species differed in their response to encounters with the horseback safari ride. Equine response behaviour appeared to be influenced by the species of game encountered. Horses seemed more wary of giraffe than other species, with a higher percentage of horses showing stationary and retreat behaviour at the start of giraffe encounters. They were also most likely to shy at giraffe. The behavioural responses suggest that game encounters can elicit a stress response in both animal groups, although it is not usually extreme, potentially indicating that some degree of habituation has occurred. Balancing the welfare of both the horses and the plains game species along with tourist preferences may be challenging in this context.
The Reproductive Success of Triturus ivanbureschi × T. macedonicus F1 Hybrid Females (Amphibia: Salamandridae)
Authors: Vučić Tijana , Ivanović Ana , Ajduković Maja , Bajler Nikola , Cvijanović Milena
Published: February 2022

Two large-bodied newt species, Triturus ivanbureschi and T. macedonicus, hybridize in nature across the Balkan Peninsula. Consequences of hybridization upon secondary contact of two species include species displacement and asymmetrical introgression of T. ivanbureschi mtDNA. We set an experimental reciprocal cross of parental species and obtained two genotypes of F1 hybrids (with T. ivanbureschi or T. macedonicus mtDNA). When hybrids attained sexual maturity, they were engaged in mutual crossings and backcrossing with parental species. We followed reproductive traits over two successive years. Our main aim was to explore the reproductive success of F1 females carrying different parental mtDNA. Additionally, we tested for differences in reproductive success within female genotypes depending on the crossing with various male genotypes (hybrids or parental species). Both female genotypes had similar oviposition periods, number of laid eggs and hatched larvae but different body and egg sizes. Overall reproductive success (percentage of egg-laying females and viability of embryos) was similar for both genotypes. The type of crossing led to some differences in reproductive success within female genotypes. The obtained results suggest that processes that led to exclusion of T. macedonicus mtDNA in natural populations may be related to the survival at postembryonic stages of F2 generation or reproductive barriers that emerged in subsequent hybrid generations.
Investigation of Copy Number Variations (CNVs) of the Goat PPP3CA Gene and Their Effect on Litter Size and Semen Quality
Authors: Bai Yangyang , Zhang Taiyuan , Liu Nuan , Wang Congliang , Guo Zhengang , Pan Chuanying , Zhu Haijing , Lan Xianyong
Published: February 2022

Copy number variations (CNVs) have many forms of variation structure, and they play an important role in the research of variety diversity, biological evolution and disease correlation. Since CNVs have a greater impact on gene regulation and expression, more studies are being finalized on CNVs in important livestock and poultry species. The protein phosphatase 3 catalytic subunit alpha (PPP3CA) is a key candidate gene involved in the goat fecundity trait, and has important effects on precocious puberty, estrogen signal transduction pathways and oocyte meiosis. Additionally, PPP3CA also has a dephosphorylation effect in the process of spermatogonial stem cell meiosis and spermatogenesis. So far, there is no research on the relationship between the copy number variations of the PPP3CA gene and reproduction traits. Therefore, the purpose of this study was to determine the association between copy number variations in the goat PPP3CA gene and litter size and semen quality in Shaanbei white cashmere goats (SBWC) (n = 353) and Guizhou Heima goats (n = 64). Based on the association analysis, the results showed that only CNV1 and CNV2 within the PPP3CA gene were distinctly related to the first-birth litter size in female goats (p = 7.6802 × 10−11; p = 5.0895 × 10−9, respectively) and they were also significantly associated with the semen quality of SBWC goats (p < 0.05). In addition, individuals with Loss genotypes demonstrated better phenotypic performance compared to those with other types. Therefore, CNV1 and CNV2 of the PPP3CA gene are potentially useful for breeding, as they are linked to important goat reproduction traits.
Host–Parasite Relationships in Veterinary Parasitology: Get to Know Your Enemy before Fighting It
Authors: González-Miguel Javier
Published: February 2022

The evolutionary success of parasitism is directly related to the huge number of species that have evolved this way of life [...]
Epidemiology of Porcine Circovirus Type 2 Circulating in Wild Boars of Portugal during the 2018–2020 Hunting Seasons Suggests the Emergence of Genotype 2d
Authors: de Sousa Moreira Alícia , Santos-Silva Sérgio , Mega João , Palmeira Josman D. , Torres Rita T. , Mesquita João R.
Published: February 2022

Porcine circovirus type 2 (PCV-2) is associated with several syndromes affecting swine, also known as porcine-circovirus-associated diseases, of which post-weaning multi-systemic wasting syndrome stands out due to its high economic impact on swine production. Recent data suggest the increasing circulation of the PCV-2d genotype in several countries worldwide. To provide updated data on PCV-2 genotypes currently circulating in swine in Portugal, we screened wild boar stools collected from several districts across Portugal, during the 2018–2020 hunting seasons, for PCV-2 and genetically characterized detected strains. From a total of 76 stool samples of wild boar tested by PCR for the partial PCV-2 ORF2 gene, two sequences were obtained (2/76; 2.6%, 95% confidence interval: 0.032–9.18). Bidirectional sequencing showed that the sequences were 100% identical and both of the PCV-2d genotype, showing for the first time the presence of this genotype in Portugal. Monitoring wild PCV-2 reservoirs is important for both veterinary public health and economic reasons, since PCV-2 infection has a strong economic impact on the swine industry.
Multi-Center Agent Loss for Visual Identification of Chinese Simmental in the Wild
Authors: Zhao Jianmin , Lian Qiusheng , Xiong Neal N.
Published: February 2022

Visual identification of cattle in the wild provides an essential way for real-time cattle monitoring applicable to precision livestock farming. Chinese Simmental exhibit a yellow or brown coat with individually characteristic white stripes or spots, which makes a biometric identifier for identification possible. This work employed the observable biometric characteristics to perform cattle identification with an image from any viewpoint. We propose multi-center agent loss to jointly supervise the learning of DCNNs by SoftMax with multiple centers and the agent triplet. We reformulated SoftMax with multiple centers to reduce intra-class variance by offering more centers for feature clustering. Then, we utilized the agent triplet, which consisted of the features and the agents, to enforce separation among different classes. As there are no datasets for the identification of cattle with multi-view images, we created CNSID100, consisting of 11,635 images from 100 Chinese Simmental identities. Our proposed loss was comprehensively compared with several well-known losses on CNSID100 and OpenCows2020 and analyzed in an engineering application in the farming environment. It was encouraging to find that our approach outperformed the state-of-the-art models on the datasets above. The engineering application demonstrated that our pipeline with detection and recognition is promising for continuous cattle identification in real livestock farming scenarios.
Icariin in Combination with Amoxycillin-Clavulanate and Ampicillin, but Not Vancomycin, Increases Antibiotic Sensitivity and Growth Inhibition against Methicillin-Resistant Staphylococcus aureus
Authors: Peris María Cardells , Martínez Alba , Ortíz Marina Pascual , Sheth Chirag C. , Veses Veronica
Published: February 2022

The widespread irrational use of antibiotics in recent years has resulted in an increase in the detection of multi-resistant bacterial strains, particularly methicillin-resistant Staphylococcus aureus (MRSA). The use of natural derivatives such as flavonoids is postulated as one of the most promising avenues to solve this emerging public health problem. The objective of the present study is to characterize the antimicrobial activity of icariin, a flavonoid compound isolated from a variety of plants of the Epimedium genus, against human and animal clinical MRSA isolates. Our study found that icariin alone did not have any antimicrobial effect on S. aureus or MRSA clinical isolates. However, icariin enhanced the effect of amoxycillin-clavulanate or ampicillin, whereas no effect was seen when used in combination with vancomycin. Specifically, co-incubation of S. aureus with amoxycillin-clavulanate plus icariin resulted in an increased proportion of dead cells, suggesting that this flavonoid potentially increases antimicrobial activity when used in combination with the beta-lactam antibiotic amoxycillin-clavulanate. Furthermore, we demonstrate that co-incubation of S. aureus with AmoxyClav plus icariin resulted in increased membrane disruption and growth inhibition. This study demonstrates the potential utility of icariin in permitting lower antibiotic therapeutic doses in alignment with strategies to reduce the spread of antibiotic resistance. Further research is required to determine the optimum concentration of icariin and to define clinically relevant combinations of flavonoid and antibiotic.
Antimicrobial and Antibiofilm Coating of Dental Implants—Past and New Perspectives
Authors: Esteves Guilherme Melo , Esteves João , Resende Marta , Mendes Luzia , Azevedo Andreia S.
Published: February 2022

Regarded as one of the best solutions to replace missing teeth in the oral cavity, dental implants have been the focus of plenty of studies and research in the past few years. Antimicrobial coatings are a promising solution to control and prevent bacterial infections that compromise the success of dental implants. In the last few years, new materials that prevent biofilm adhesion to the surface of titanium implants have been reported, ranging from improved methods to already established coating surfaces. The purpose of this review is to present the developed antimicrobial and antibiofilm coatings that may have the potential to reduce bacterial infections and improve the success rate of titanium dental implants. All referred coating surfaces showed high antimicrobial properties with effectiveness in biofilm control, while maintaining implant biocompatibility. We expect that by combining the use of oligonucleotide probes as a covering material with novel peri-implant adjuvant therapies, we will be able to avoid the downsides of other covering materials (such as antibiotic resistance), prevent bacterial infections, and raise the success rate of dental implants. The existing knowledge on the optimal coating material for dental implants is limited, and further research is needed before more definitive conclusions can be drawn.
Nocturnal Birds of Prey as Carriers of Staphylococcus aureus and Other Staphylococci: Diversity, Antimicrobial Resistance and Clonal Lineages
Authors: Silva Vanessa , Lopes Ana Filipa , Soeiro Vanessa , Caniça Manuela , Manageiro Vera , Pereira José Eduardo , Maltez Luís , Capelo José Luis , Igrejas Gilberto , Poeta Patrícia
Published: February 2022

Owls are nocturnal predators that inhabit urbanized and farmlands. They are in direct contact with other animals, both livestock and small wild rodents that they mostly feed on. Staphylococci can be both commensal and pathogenic bacteria that are widespread across the various ecological niches. We aimed to isolate staphylococci from owls and to characterize their antimicrobial resistance, virulence factors and genetic lineages. Swab samples were collected from the throat and cloaca of 114 owls admitted to two rehabilitation centers in Portugal. The identification of staphylococci species was performed by MALDI-TOF. Staphylococci antimicrobial resistance and virulence genes were investigated by means of the disk diffusion method and PCR. Staphylococcus aureus isolates were characterized by MLST, agr and spa-typing. Of the tested animals, 66 isolates were recovered, including 10 different species of staphylococci, of which 25 were coagulase-positive (CoPS) and 41 were coagulase-negative (CoNS). Twenty-three S. aureus were isolated, of which one mecC-MRSA was identified. The isolates were mainly resistant to penicillin, aminoglycosides, clindamycin and tetracycline. mecC-MRSA belonged to ST1245 and spa-type t843 and the remaining S. aureus were ascribed to 12 STs and 15 spa types. A high diversity of clonal lineages was identified among the S. aureus isolated from wild owls. Owls feed mainly on small rodents often exposed to waste and anthropogenic sources, which may explain the moderate prevalence of S. aureus in these animals.