bluuebunny/crossref_metadata_2025 huggingface dataset:
Ruxolitinib inhibits cytokine production by human lung macrophages without impairing phagocytic ability
Authors: Mantov Nikola , Zrounba Mathilde , Brollo Marion , Grassin-Delyle S , Glorion Matthieu , David Mélanie , Naline Emmanuel , Devillier Philippe , Salvator Hélène
Published: August 2022

Background: The Janus kinase (JAK) 1/2 inhibitor ruxolitinib has been approved in an indication of myelofibrosis and is a candidate for the treatment of a number of inflammatory or autoimmune diseases. We assessed the effects of ruxolitinib on lipopolysaccharide (LPS)- and poly (I:C)-induced cytokine production by human lung macrophages (LMs) and on the LMs’ phagocytic activity.

Methods: Human LMs were isolated from patients operated on for lung carcinoma. The LMs were cultured with ruxolitinib (0.5 × 10−7 M to 10–5 M) or budesonide (10–11 to 10–8 M) and then stimulated with LPS (10 ng·ml−1) or poly (I:C) (10 μg·ml−1) for 24 h. Cytokines released by the LMs into the supernatants were measured using ELISAs. The phagocytosis of labelled bioparticles was assessed using flow cytometry.

Results: Ruxolitinib inhibited both the LPS- and poly (I:C)-stimulated production of tumor necrosis factor alpha, interleukin (IL)-6, IL-10, chemokines CCL2, and CXCL10 in a concentration-dependent manner. Ruxolitinib also inhibited the poly (I:C)- induced (but not the LPS-induced) production of IL-1ß. Budesonide inhibited cytokine production more strongly than ruxolitinib but failed to mitigate the production of CXCL10. The LMs’ phagocytic activity was not impaired by the highest tested concentration (10–5 M) of ruxolitinib.

Conclusion: Clinically relevant concentrations of ruxolitinib inhibited the LPS- and poly (I:C)-stimulated production of cytokines by human LMs but did not impair their phagocytic activity. Overall, ruxolitinib’s anti-inflammatory activities are less intense than (but somewhat different from) those of budesonide—particularly with regard to the production of the corticosteroid-resistant chemokine CXCL-10. Our results indicate that treatment with a JAK inhibitor might be a valuable anti-inflammatory strategy in chronic obstructive pulmonary disease, Th1-high asthma, and both viral and non-viral acute respiratory distress syndromes (including coronavirus disease 2019).
Qu-Du-San-Jie decoction induces growth inhibition and vascular normalization in NF2-associated vestibular schwannoma
Authors: Lin Jie , Li Shi-Wei , Zhang Jing , Chu Fu-Hao , Li Cheng-Ze , Bie Zhi-Xu , Tang Han-Lu , Gao Shan , Li Ping , Liao Meng-Ting , Xin Tian-Xi , Zhao Fu , Liu Pi-Nan , Ding Xia
Published: August 2022

Background: Neurofibromatosis type 2 (NF2) is a rare genetic syndrome that predisposes individuals to develop bilateral vestibular schwannomas (VSs) causing a high risk of life-threatening neurological complications. Traditional treatment options for NF2-associated VS usually cause neurological damage, and to date, there are no FDA-approved pharmacotherapies for NF2. The aim of this study was to evaluate the antitumor efficacy of Qu-Du-San-Jie (QDSJ) decoction, a traditional Chinese medicine formula, on NF2-associated VS and to investigate the potential underlying mechanisms.

Methods: Ultra high-performance liquid chromatography-mass spectroscopy (UHPLC-MS) analysis was performed to identify the components of QDSJ and their targets. To determine the relationships between the putative targets of QDSJ and the differential genes of NF2-associated VS, the drug-disease crossover genes were screened using the UHPLC-MS data combined with our previous gene expression profiling data. The differentially expressed genes were imported into the STRING database to generate a PPI network. Differentially expressed gene targets and pathways were identified using GO and KEGG pathway enrichment analyses. The in vitro and in vivo drug efficacy of QDSJ decoction was tested using a patient-derived schwannoma cell line and a patient-derived xenograft mouse model, respectively. H&E staining, immunochemistry, and immunofluorescence staining were used to evaluate the cell proliferation and tumor vessels.

Results: A total of 133 compounds were identified in QDSJ decoction using UHPLC-MS analysis. Network pharmacology showed that the regulation of necroptosis, apoptosis, cell cycle, angiogenesis, adherens junction, and neuroactive ligand-receptor interaction could be associated with the efficacy of QDSJ in treating NF2-associated VS. Treatment with QDSJ induced necrotic cell death and apoptosis of schwannoma cells in vitro and suppressed the tumor growth in vivo. Histopathological analysis revealed areas of cell necrosis and enlarged tumor blood vessels in the QDSJ-treated tumors. The numbers of cells positive for Cyclin D1 and Ki-67 were significantly reduced in QDSJ-treated tumors compared to control tumors. Immunofluorescence staining of CD31 and αSMA showed a decreased number and density of tumor vessels and normalized vessel structure in QDSJ-treated tumors.

Conclusion: Our study demonstrates that QDSJ decoction shows significant antitumor activity against NF2-associated schwannoma and is a possible candidate for future clinical trials.
A single-dose, randomized, open-labeled, parallel-group study comparing the pharmacokinetics, pharmacodynamics and safety of leuprolide acetate microspheres 3.75 mg and Enantone® 3.75 mg in healthy male subjects
Authors: Hu Xingjiang , Zhang Qiao , Zheng Yunliang , Zhai You , Xu Nana , Zhao Qingwei , Liu Jian , Wan Longyan , Luo Jindan
Published: August 2022

Leuprolide acetate microspheres developed by Shanghai Livzon Pharmaceutical Co., Ltd. (T) have been marketed in China for more than 10 years, benefiting a large number of patients, and will continue to play an important role in China. However, as a generic drug, it is unclear whether there is a difference in efficacy between T and the original product Enantone® (R). This study compared the differences in efficacy and safety of two 1-month depot formulations in 48 healthy Chinese male subjects by comparing multiple pharmacokinetic (PK) and pharmacodynamic (PD) parameters. The main research indicators were the PK parameters of leuprolide (Cmax, AUC0-t, AUC0-D7, and AUCD7-t) and the PD parameters of testosterone (Emax, AUEC0-t, AUEC0-D7, and AUECD7-t) after 42 days of administration. The Cmax, AUC0-t, AUC0-D7 and AUCD7-t of leuprolide were slightly higher in the T group than in the R group with 90% confidence intervals (CIs) of 94.43–118.53%, 109.13–141.88%, 109.53–139.54%, and 105.17–145.74%, respectively. No significant differences in the PD parameters (Emax, AUEC0-t, AUEC0-D7, and AUECD7-t) existed between the T and R groups, and 90% CIs were 62.80–93.57%, 88.17–110.55, 95.72%–118.50%, and 79.77–105.63, respectively. At 672 h (D28), the castration rate of T was 91.30% (21/23) and that of R was 60.87% (14/23). The PK characteristics were consistent and the inhibitory effects on testosterone levels were similar in both T and R groups; further, clinical safety was observed for both T and R formulations, suggesting that these two products can replace each other in clinical practice.

Clinical Trial Registration:http://www.chinadrugtrials.org.cn/clinicaltrials.searchlistdetail.dhtml, identifier CTR20200641.
Metabolomics profiles in acute-on-chronic liver failure: Unveiling pathogenesis and predicting progression
Authors: Liu Guofeng , Wang Xiaoze , Fan Xiaoli , Luo Xuefeng
Published: August 2022

Acute-on-chronic liver failure (ACLF) usually develops based on acute decompensation (AD) of cirrhosis and is characterized by intense systemic inflammation, multiple organ failure, and high short-term mortality. Validated biomarkers for the diagnosis and prognosis of ACLF remain to be clarified. Metabolomics is an emerging method used to measure low-molecular-weight metabolites and is currently frequently implemented to understand pathophysiological processes involved in disease progression, as well as to search for new diagnostic or prognostic biomarkers of various disorders. The characterization of metabolites in ACLF has recently been described via metabolomics. The role of metabolites in the pathogenesis of ACLF deserves further investigation and improvement and could be the basis for the development of new diagnostic and therapeutic strategies. In this review, we focused on the contributions of metabolomics on uncovering metabolic profiles in patients with ACLF, the key metabolic pathways that are involved in the progression of ACLF, and the potential metabolite-associated therapeutic targets for ACLF.
Postponement of the opacification of lentoid bodies derived from human induced pluripotent stem cells after lanosterol treatment—the first use of the lens aging model in vitro in cataract drug screening
Authors: Zhang Lifang , Qin Zhenwei , Lyu Danni , Lu Bing , Chen Zhijian , Fu Qiuli , Yao Ke
Published: August 2022

Purpose: Our previous study observed that human induced pluripotent stem cell (HiPSC)-derived lentoid bodies (LBs) became cloudy with extended culture time, partially mimicking the progress of human age-related cataracts (ARCs) in a dish. In the present study, lanosterol, a potential anticataract drug, was used to further verify the value of this model in drug screening for cataract treatment.

Methods: Mature LBs on day 25, which were differentiated from HiPSCs using the “fried egg” method, were continually cultured and treated with either dimethyl sulfoxide (control) or lanosterol. The LBs’ shape and opacity alterations were examined using light microscopy and mean gray value evaluation. The soluble and insoluble proteins were examined through SDS-PAGE gel electrophoresis combined with Coomassie blue staining. The protein aggregations were examined with immunofluorescence.

Results: The mature LBs became cloudy with an extended culture time, and the opacification of the LBs was partially prevented by lanosterol treatment. There was less increase in insoluble proteins in the lanosterol-treated LBs than in the control group. There were also fewer cells containing aggregated protein (αA‐crystallin and αB‐crystallin) puncta in the lanosterol-treated LBs than in the control LBs.

Conclusion: It was found that the opacification of LBs could be delayed by lanosterol treatment, which could be achieved by reducing protein aggregation, suggesting a promising HiPSC-derived drug-screening model for Age-related cataract.
Cysteamine affects skeletal development and impairs motor behavior in zebrafish
Authors: Chen Chao , Zheng Yongliang , Li Xue , Zhang Li , Liu Kangyu , Sun Sujie , Zhong Zilin , Hu Hongmei , Liu Fasheng , Xiong Guanghua , Liao Xinjun , Lu Huiqiang , Bi Yanlong , Chen Jianjun , Cao Zigang
Published: August 2022

Cysteamine is a kind of feed additive commonly used in agricultural production. It is also the only targeted agent for the treatment of cystinosis, and there are some side effects in clinical applications. However, the potential skeletal toxicity remains to be further elucidated. In this study, a zebrafish model was for the first time utilized to synthetically appraise the skeletal developmental defects induced by cysteamine. The embryos were treated with 0.35, 0.70, and 1.05 mM cysteamine from 6 h post fertilization (hpf) to 72 hpf. Substantial skeletal alterations were manifested as shortened body length, chondropenia, and abnormal somite development. The results of spontaneous tail coiling at 24 hpf and locomotion at 120 hpf revealed that cysteamine decreased behavioral abilities. Moreover, the level of oxidative stress in the skeleton ascended after cysteamine exposure. Transcriptional examination showed that cysteamine upregulated the expression of osteoclast-related genes but did not affect osteoblast-related genes expression. Additionally, cysteamine exposure caused the downregulation of the Notch signaling and activating of Notch signaling partially attenuated skeletal defects. Collectively, our study suggests that cysteamine leads to skeletal developmental defects and reduces locomotion activity. This hazard may be associated with cysteamine-mediated inhibition of the Notch signaling and disorganization of notochordal cells due to oxidative stress and apoptosis.
Dual solutions for double diffusion and MHD flow analysis of micropolar nanofluids with slip boundary condition
Authors: Khan Y. , Majeed Afraz Hussain , Rasheed Muhammad Afraz , Alameer A. , Shahzad Hasan , Irshad Sadia , Faraz N.
Published: August 2022

The present communication is designed to elucidate the flow attributes of micro-polar non-Newtonian liquid over stretching/shrinking surfaces. In addition, we have observed the stagnation aspect along with the velocity slip condition on the momentum field. The Fourier law of heat conduct, along with a physical aspect of stratified and heat generation absorption, are then used to model the temperature equation. The Buongiorno nanofluid model is used to study additional transport features. After a discussion of PDEs using similarity transformation, mathematical formulations of the given problem are supported in the form of an ordinary differential system. The solution of modeled governing equations containing physical effects is simulated by using the shooting method in conjunction with RK- Method. The significant effects of flow parameters that are associated with velocity, temperature, and concentration distribution for low and upper branch solutions are revealed through graphs and tables. Quantities of engineering concerns like skin friction coefficient and Nusselt number are also compared with previous results of critical values. Furthermore, it should be considered that as the micro-pole parameters are increased, the local skin friction coefficient and the local Nusselt number amplitude also rise.
Traceability of optical frequency to SI second via UTC(NIM)
Authors: Wang Yuzhuo , Lin Yige , Gao Yuan , Zhang Aimin
Published: August 2022

Absolute frequency measurement of an optical clock is a milestone towards the redefinition of second in the International System of Units. This work summarizes briefly the measurement of frequency ratio between an optical clock and the second, applying the frequency comparison link from UTC(NIM) to International Atomic Time. Some strategies and suggestions are proposed to reduce the uncertainty introduced by the measurement system and dead time, which guided the frequency measurement of three optical clocks at 10−16 level in the past 3 years.
High-Isolation wideband MIMO antenna with offset T-Shaped slots for 5G/WLAN applications
Authors: Chen Shuyi , Lu Na , Sun Jiaxing , Zhou Changfei , Li Changxian , Wu Di
Published: August 2022

A broadband multiple-input multiple-output (MIMO) antenna with high isolation operating in 3.14–5.42 GHz (2.28 GHz, 53.3%) is proposed for 5G smartphones. An offset T-shaped slot with dual functions is employed to improve the bandwidth of one antenna element and enhance the isolation. Then, the double L-shaped stubs are loaded to improve the impedance matching of the other antenna in the MIMO antenna. Finally, the 2 × 2 MIMO antenna is constructed by four T-shaped monopole antennas, two double L-shaped stubs, and two offset T-shaped slots. The proposed MIMO antenna is first simulated and then experimentally tested with a good agreement. High isolation of greater than 15 dB, high antenna efficiency of more than 54%, and low envelope correlation coefficient (ECC) of smaller than 0.08 are realized within 3.14–5.42 GHz. These good performances indicate that the proposed antenna can be applied for MIMO applications in smartphones.
System level modeling and analysis of TNF-α mediated sphingolipid signaling pathway in neurological disorders for the prediction of therapeutic targets
Authors: Banaras Sanam , Paracha Rehan Zafar , Nisar Maryum , Arif Ayesha , Ahmad Jamil , Tariq Saeed Muhammad , Mustansar Zartasha , Shuja Malik Nawaz , Paracha Rizwan Nasir
Published: August 2022

Sphingomyelin (SM) belongs to a class of lipids termed sphingolipids. The disruption in the sphingomyelin signaling pathway is associated with various neurodegenerative disorders. TNF-α, a potent pro-inflammatory cytokine generated in response to various neurological disorders like Alzheimer’s disease (AD), Parkinson’s disease (PD), and Multiple Sclerosis (MS), is an eminent regulator of the sphingomyelin metabolic pathway. The immune-triggered regulation of the sphingomyelin metabolic pathway via TNF-α constitutes the sphingomyelin signaling pathway. In this pathway, sphingomyelin and its downstream sphingolipids activate various signaling cascades like PI3K/AKT and MAPK/ERK pathways, thus, controlling diverse processes coupled with neuronal viability, survival, and death. The holistic analysis of the immune-triggered sphingomyelin signaling pathway is imperative to make necessary predictions about its pivotal components and for the formulation of disease-related therapeutics. The current work offers a comprehensive in silico systems analysis of TNF-α mediated sphingomyelin and downstream signaling cascades via a model-based quantitative approach. We incorporated the intensity values of genes from the microarray data of control individuals from the AD study in the input entities of the pathway model. Computational modeling and simulation of the inflammatory pathway enabled the comprehensive study of the system dynamics. Network and sensitivity analysis of the model unveiled essential interaction parameters and entities during neuroinflammation. Scanning of the key entities and parameters allowed us to determine their ultimate impact on neuronal apoptosis and survival. Moreover, the efficacy and potency of the FDA-approved drugs, namely Etanercept, Nivocasan, and Scyphostatin allowed us to study the model’s response towards inhibition of the respective proteins/enzymes. The network analysis revealed the pivotal model entities with high betweenness and closeness centrality values including recruit FADD, TNFR_TRADD, act CASP2, actCASP8, actCASP3 and 9, cytochrome C, and RIP_RAIDD which profoundly impacted the neuronal apoptosis. Whereas some of the entities with high betweenness and closeness centrality values like Gi-coupled receptor, actS1PR, Sphingosine, S1P, actAKT, and actERK produced a high influence on neuronal survival. However, the current study inferred the dual role of ceramide, both on neuronal survival and apoptosis. Moreover, the drug Nivocasan effectively reduces neuronal apoptosis via its inhibitory mechanism on the caspases.
Sickle cell cerebrovascular reactivity to a CO2 stimulus: Too little, too slow
Authors: Forté Stéphanie , Sobczyk Olivia , Poublanc Julien , Duffin James , Hare Gregory M. T. , Fisher Joseph Arnold , Mikulis David , Kuo Kevin H. M.
Published: August 2022

Background: Despite increased cerebral blood flow (CBF), cerebral infarcts occur in patients with sickle cell disease (SCD). This suggests increased CBF does not meet metabolic demand possibly due to compromised cerebral vasodilatory response. Hypothesis: In adult SCD patients, cerebrovascular reactivity (CVR) and speed of vasodilatory response (tau) to a standardized vasodilatory stimulus, are reduced compared to normal subjects.

Methods: Functional brain imaging performed as part of routine care in adult SCD patients without known large vessel cerebral vasculopathy was reviewed retrospectively. CVR was calculated as the change in CBF measured as the blood-oxygenation-level-dependent (BOLD)-magnetic resonance imaging signal, in response to a standard vasoactive stimulus of carbon dioxide (CO2). The tau corresponding to the best fit between the convolved end-tidal partial pressures of CO2 and BOLD signal was defined as the speed of vascular response. CVR and tau were normalized using a previously generated atlas of 42 healthy controls.

Results: Fifteen patients were included. CVR was reduced in grey and white matter (mean Z-score for CVR −0.5 [−1.8 to 0.3] and −0.6 [−2.3 to 0.7], respectively). Tau Z-scores were lengthened in grey and white matter (+0.9 [−0.5 to 3.3] and +0.8 [−0.7 to 2.7], respectively). Hematocrit was the only significant independent predictor of CVR on multivariable regression.

Conclusion: Both measures of cerebrovascular health (CVR and tau) in SCD patients were attenuated compared to normal controls. These findings show that CVR represents a promising tool to assess disease state, stroke risk, and therapeutic efficacy of treatments in SCD and merits further investigation.
Effects of footwear with different longitudinal bending stiffness on biomechanical characteristics and muscular mechanics of lower limbs in adolescent runners
Authors: Chen Hairong , Shao Enze , Sun Dong , Xuan Rongrong , Baker Julien S. , Gu Yaodong
Published: August 2022

Background: Running shoes with carbon plates have been identified to have positive effects on improving running performance from a biomechanical perspective. However, the specific difference between the effects of carbon plates with different longitudinal bending stiffness (LBS)on biomechanical characteristics and muscular mechanics of lower limbs in adolescent runners remains unclear. This study aimed to identify the difference in biomechanical characteristics and muscular mechanics in lower limbs during running stance phases between wearing shoes with low longitudinal bending stiffness (Llbs) and high longitudinal bending stiffness (Hlbs) carbon plates in adolescent runners.

Methods: 10 male adolescent runners with a habit of daily running exercise (age: 13.5 ± 0.6 years; height: 166.3 ± 1.9 cm; bodyweight: 50.8 ± 3.1 kg; foot length: 25.4 ± 0.2 cm) were recruited and asked to conduct two times of tests by wearing shoes with Llbs and Hlbs carbon plates in a randomized order. Paired t-test and statistical parametric mapping (SPM) analysis were used to identify the difference in biomechanical characteristics and muscular mechanics in lower limbs during running stance phases.

Result: Under the condition of wearing shoes with Hlbs, the time of foot contact significantly increased, whereas the range of motion (ROM) of hip and metatarsophalangeal (MTP) in the sagittal plane significantly reduced as well as the peak moment of ankle joint in the sagittal plane. The activations of vastus medialis, vastus lateralis, flexor digitorum brevis (flex dig brevis), and flexor hallucis longus (flex hall long) significantly increased under the condition of wearing shoes with Hlbs. According to the results of the SPM analysis, the joint angles (hip, ankle, and MTP), the net joint moments (knee, ankle, and MTP), and the muscle forces (gluteus maximus and tibialis anterior) were significant difference during the running stance phase between conditions of wearing shoes with Hlbs and Llbs.

Conclusion: Running shoes with Llb carbon plates are appropriate for adolescent runners due to the advantages of biomechanical characteristics and muscular mechanics.
gH625-liposomes deliver PACAP through a dynamic in vitro model of the blood–brain barrier
Authors: Barra Teresa , Falanga Annarita , Bellavita Rosa , Laforgia Vincenza , Prisco Marina , Galdiero Stefania , Valiante Salvatore
Published: August 2022

The blood–brain barrier (BBB) selectively protects the central nervous system (CNS) from external insults, but its function can represent a limit for the passage of therapeutic molecules. Numerous in vitro models of the BBB have been realized in order to study the passage of drugs for neurodegenerative diseases, but these in vitro models are not very representative of the physiological conditions because of a limited supply of oxygen and nutrients due to static conditions. To avoid this phenomenon, we used a millifluidic bioreactor model that ensures a circulation of the medium and, therefore, of the nutrients, thanks to the continuous laminar flow. This dynamic model consists of a double-culture chamber separated by a membrane on which brain endothelial cells are cultured in order to evaluate the passage of the drug. Furthermore, in the lower chamber, SH-SY5Y were seeded as 3D spheroids to evaluate the drug passage through these cells. As nanodelivery system, we used liposomes functionalized with viral fusion peptide to evaluate the passage of a neuroprotective agent, pituitary adenylate cyclase-activating polypeptide (PACAP), through the dynamic in vitro model of the BBB. We showed that our nanodelivery system, made of functionalized liposomes and loaded with specific molecules, efficiently crosses the in vitro fluid-dynamic model of the BBB. Our findings represent an important step for further experimental investigations on PACAP administration as a therapeutic agent by an enhanced drug delivery system. Our results can improve the diffusion of good practice in neuroscience laboratories, helping to spread the 3R rules.
Compensatory effects of different exercise durations on non-exercise physical activity, appetite, and energy intake in normal weight and overweight adults
Authors: Liu Xiao-Mei , Wang Ke , Zhu Zheng , Cao Zhen-Bo
Published: August 2022

Objectives: To examine compensatory changes of different exercise durations on non-exercise physical activity (NEPA), appetite, and energy intake (EI) in normal and overweight adults, and to determine if different body mass index of individuals interact with these compensatory effects.

Methods: Ten normal weight adults (nine females and one male; age: 24.0 ± 0.4 years; BMI: 20.7 ± 0.5 kg/m2) and ten overweight adults (six females and four males; age: 24.5 ± 0.9 years; BMI: 25.9 ± 0.4 kg/m2) participated in this study. The participants completed two exercise trials: short-duration continuous training (SDCT) and long-duration continuous training (LDCT), i.e., a 40 min short-duration and an 80 min long-duration continuous training in a randomized order. Total physical activity and NEPA were monitored using an accelerometer for seven consecutive days, which involved a two-day baseline observation period (C-pre-Ex), three-day exercise intervention period (Ex), and two-day follow-up period (C-post-Ex). Blood samples were collected for appetite-related hormone analysis. Appetite score was assessed using the visual analogue scale. Energy intake was evaluated by weighing the food and recording diaries.

Results: The NEPA evaluation showed that it was higher for SDCT than for LDCT in the C-post-Ex period (F (1, 19) = 8.508, p = 0.009) in the total sample. Moreover, results also indicated that NEPA was lower for LDCT (F (2, 18) = 6.316, p = 0.020) and higher for SDCT (F (2, 18) = 3.889, p = 0.026) in the C-post-Ex period than in the C-pre-Ex and Ex periods in overweight group. Acyl-ghrelin revealed a main effect of time in the total sample and in normal weight and overweight groups; it was lower in the C-post-Ex period than in the C-pre-Ex and Ex periods (all p < 0.05). Total EI analysis revealed no significant changes in either the total sample or in the normal weight and overweight groups.

Conclusion: These findings demonstrate that short duration exercise led to a compensatory increment in NEPA, whereas long duration exercise induced a compensatory decrease in NEPA. Moreover, there was a higher and delayed compensatory response in overweight adults than in normal weight adults. Nevertheless, energy intake was not changed across time, regardless of exercise duration.
Fetal growth restriction and stillbirth: Biomarkers for identifying at risk fetuses
Authors: King Victoria J. , Bennet Laura , Stone Peter R. , Clark Alys , Gunn Alistair J. , Dhillon Simerdeep K.
Published: August 2022

Fetal growth restriction (FGR) is a major cause of stillbirth, prematurity and impaired neurodevelopment. Its etiology is multifactorial, but many cases are related to impaired placental development and dysfunction, with reduced nutrient and oxygen supply. The fetus has a remarkable ability to respond to hypoxic challenges and mounts protective adaptations to match growth to reduced nutrient availability. However, with progressive placental dysfunction, chronic hypoxia may progress to a level where fetus can no longer adapt, or there may be superimposed acute hypoxic events. Improving detection and effective monitoring of progression is critical for the management of complicated pregnancies to balance the risk of worsening fetal oxygen deprivation in utero, against the consequences of iatrogenic preterm birth. Current surveillance modalities include frequent fetal Doppler ultrasound, and fetal heart rate monitoring. However, nearly half of FGR cases are not detected in utero, and conventional surveillance does not prevent a high proportion of stillbirths. We review diagnostic challenges and limitations in current screening and monitoring practices and discuss potential ways to better identify FGR, and, critically, to identify the “tipping point” when a chronically hypoxic fetus is at risk of progressive acidosis and stillbirth.
Coupling the environmental impacts of reactive nitrogen losses and yield responses of staple crops in China
Authors: Abdo Ahmed I. , Sun Daolin , Li Yazheng , Yang Jiayue , Metwally Mohamed S. , Abdel-Hamed Enas M. W. , Wei Hui , Zhang Jiaen
Published: August 2022

Cropland reactive nitrogen losses (Nr) are of the greatest challenges facing sustainable agricultural intensification to meet the increases in food demand. The environmental impacts of Nr losses and their yield responses to the mitigation strategies were not completely evaluated. We assessed the environmental impacts of Nr losses in China and coupled the efficiency of mitigation actions with yield responses. Datasets about Nr losses in China were collected, converted into potentials of acidification (AP), global warming (GWP), and aquatic eutrophication (AEP), and analyzed by a meta-analysis program. Results showed that producing 1 Mg of rice grains had the highest AP (153 kg acid equiv.), while wheat had the highest GWP and AEP (74 kg CO2 equiv. and 0.37 kg PO4 equiv., respectively). Using the conventional rates (averagely, 200, 230, and 215 kg N ha−1) of urea as a surface application to produce 131.4, 257.2, and 212.1 Tg of wheat, maize, and rice resulted in 17–33 Tg, 7–10 Tg, and 6–87 Gg of AP, GWP, and AEP, respectively. For their balanced effect on reducing AP, GWP, and AEP while maximizing yields, inhibitors, and subsurface application could be set as the best mitigation strategies in wheat production. Inhibitors usage and biochar are strongly recommended strategies for sustainable production of maize. None of the investigated strategies had a balanced effect on rice yield and the environment, thus new mitigation technologies should be developed.
Investigating the differences between females perceive same-gender and heterogender sex robots regarding adoption and intentions
Authors: Li Yuanjun
Published: August 2022

The market for sex robots is on the rise with the development of human–computer interaction. However, most sex robots on the market are presented as male-friendly products. This issue may limit and hinder females' adoption and utilization of sex robots. This paper was to take females as the research subjects exploring and verifying several concerns based on previous theories and to conduct primary research and quantitative method to investigate: (i) how females differently perceive same-gender and heterogender sex robots; (ii) their attitudes and the knowledge or definition of sex robots; and (iii) their intention of adopting heterogender robots. This study confirmed several previous theories and provided new findings and insights. Females are more likely to feel threatened by the presence of same-gender sex robots. Their negative attitudes are related to the way that sex robots exist. They are jealous of same-gender sex robots; nevertheless, this should not be attributed to their negative perception of sex robots since they also have positive perceptions and intentions to adopt a sex robot. They define sex robots more as sexual products than as engaging in the prostitution industry.
Do financial performance and firm’s value affect the quality of corporate social responsibility disclosure: Moderating role of chief executive officer’s power in China
Authors: Na Cao , Tian Gaoliang , Rauf Fawad , Naveed Khwaja
Published: August 2022

This paper investigates the correlation between the quality of corporate social responsibility disclosure (CSRD) and financial performance (FP). It also investigates the moderating role of chief executive officer power (CEOP) in the relationship between the quality of CSR disclosure and firm value (FV) in Chinese listed companies. The evidential research used the up-to-date sample (3, 248) of unbalanced findings for the period of 2014–2020, from the registered Chinese firms in the Shenzhen and Shanghai Stock Exchanges as samples for the study. As a starting point technique, the STATA 15 has been used to test pooled ordinary least squares (OLS) regression on a sample of Chinese listed companies. We use 1-year lagged regression and two SLS regressions to monitor the potential endogeneity problem. The imbalanced data set was received from the China Stock Market and Accounting Research (CSMAR) web page, which is the most significant source of information for Chinese publicly listed firms. Data on CSR information items and media reporting are compiled manually. The findings of the study revealed that there are positive FP consequences for the companies engaged in the quality of CSR disclosure. We also report that higher CEO power negatively enhances the quality of CSR disclosure effect on the FP of FV. The research investigates the impact of CSR disclosure and FP by presenting evidence of the moderating role of CEO power. Therefore, it is suggested that a higher law for CSR engagement and disclosure be implemented in China, and robust measures for the implementation of CEO power, although there are financial advantages to be gained. A key relevance to the empirical quality of CSR disclosure research can be recognized as the moderating role of CEO power in the quality of CSR disclosure, FP, and FV in the context of Chinese study. The findings are robust with the use of an instrumental variable method.
The impact of potentially modifiable risk factors for stroke in a middle-income area of China: A case-control study
Authors: Wu Yuhang , Chen Xiaoyun , Hu Songbo , Zheng Huilie , Chen Yiying , Liu Jie , Xu Yan , Chen Xiaona , Zhu Liping , Yan Wei
Published: August 2022

Aims

To reveal the impact of eleven risk factors on stroke and provide estimates of the prevention potential.

Methods

We completed a multicenter case-control study in Jiangxi, China, a middle-income area. Neuroimaging examination was performed in all cases. Controls were stroke-free adults recruited from the community in the case concentration area. Conditional logistic regression and unconditional logistic regression were used for subgroup analysis of stroke type, and other groups (sex, age and urban-rural area), respectively. Odds ratios (ORs) and their population attributable risks (PARs) were calculated, with 95% confidence intervals.

Results

A total of 43,615 participants (11,735 cases and 31,880 controls) were recruited from February to September 2018, of whom we enrolled 11,729 case-control pairs. Physical inactivity [PAR 69.5% (66.9–71.9%)] and hypertension [53.4% (49.8–56.8%)] were two major risk factors for stroke, followed by high salt intake [23.9% (20.5–27.3%)], dyslipidemia [20.5% (17.1–24.0%)], meat-based diet [17.5% (14.9–20.4%)], diabetes [7.7% (5.9–9.7%)], cardiac causes [5.3% (4.0–6.7%)], alcohol intake [4.7% (0.2–10.0%)], and high homocysteine [4.3% (1.4–7.4%)]. Nine of these factors were associated with ischemic stroke, and five were associated with intracerebral hemorrhage. Collectively, eleven risk factors accounted for 59.9% of the PAR for all stroke (ischemic stroke: 61.0%; intracerebral hemorrhage: 46.5%), and were consistent across sex (men: 65.5%; women: 62.3%), age (≤55: 65.2%; >55: 63.5%), and urban-rural areas (city: 62.2%; county: 65.7%).

Conclusion

The 11 risk factors associated with stroke identified will provide an important reference for evidence-based planning for stroke prevention in middle-income areas. There is an urgent need to improve awareness, management and control of behavioral and metabolic risk factors, particularly to promote physical activity and reduce blood pressure.
The Significance of Software Engineering to Forecast the Public Health Issues: A Case of Saudi Arabia
Authors: Al-Ahmadi Haneen Hassan
Published: August 2022

In the recent years, public health has become a core issue addressed by researchers. However, because of our limited knowledge, studies mainly focus on the causes of public health issues. On the contrary, this study provides forecasts of public health issues using software engineering techniques and determinants of public health. Our empirical findings show significant impacts of carbon emission and health expenditure on public health. The results confirm that support vector machine (SVM) outperforms the forecasting of public health when compared to multiple linear regression (MLR) and artificial neural network (ANN) technique. The findings are valuable to policymakers in forecasting public health issues and taking preemptive actions to address the relevant health concerns.
Urine metabolomics analysis of sleep quality in deep-underground miners: A pilot study
Authors: Wen Qiao , Zhou Jing , Sun Xiaoru , Ma Tengfei , Liu Yilin , Xie Yike , Wang Ling , Cheng Juan , Wen Jirui , Wu Jiang , Zou Jian , Liu Shixi , Liu Jifeng
Published: August 2022

Background

In previous questionnaire surveys of miners, sleep disorders were found among underground workers. The influence of the special deep-underground environment and its potential mechanism are still unclear. Therefore, this study intends to utilize LC-MS metabolomics to study the potential differences between different environments and different sleep qualities.

Methods

Twenty-seven miners working at 645–1,500 m deep wells were investigated in this study, and 12 local ground volunteers were recruited as the control group. The Pittsburgh Sleep Quality Index (PSQI) was used to examine and evaluate the sleep status of the subjects in the past month, and valuable basic information about the participants was collected. PSQI scores were obtained according to specific calculation rules, and the corresponding sleep grouping and subsequent analysis were carried out. Through liquid chromatography-mass spectrometry (LC-MS) non-targeted metabolomics analysis, differences in metabolism were found by bioinformatics analysis in different environments.

Results

Between the deep-underground and ground (DUvsG) group, 316 differential metabolites were identified and 125 differential metabolites were identified in the good sleep quality vs. poor sleep quality (GSQvsPSQ) group. The metabolic pathways of Phenylalanine, tyrosine and tryptophan biosynthesis (p = 0.0102) and D-Glutamine and D-glutamate metabolism (p = 0.0241) were significantly enriched in DUvsG. For GSQvsPSQ group, Butanoate metabolism was statistically significant (p = 0.0276). L-Phenylalanine, L-Tyrosine and L-Glutamine were highly expressed in the deep-underground group. Acetoacetic acid was poorly expressed, and 2-hydroxyglutaric acid was highly expressed in good sleep quality.

Conclusions

The influence of the underground environment on the human body is more likely to induce specific amino acid metabolism processes, and regulate the sleep-wake state by promoting the production of excitatory neurotransmitters. The difference in sleep quality may be related to the enhancement of glycolytic metabolism, the increase in excitatory neurotransmitters and the activation of proinflammation. L-phenylalanine, L-tyrosine and L-glutamine, Acetoacetic acid and 2-hydroxyglutaric acid may be potential biomarkers correspondingly.
Interventions for social and community participation for adults with intellectual disability, psychosocial disability or on the autism spectrum: An umbrella systematic review
Authors: Giummarra Melita J. , Randjelovic Ivana , O’Brien Lisa
Published: August 2022

Objective

This umbrella systematic review examined the effectiveness, facilitators, and barriers of interventions for social, community and civic participation for adults on the autism spectrum, or with intellectual or psychosocial disability.

Data Sources

Eight databases were searched to identify eligible reviews defined by the: Sample (≥50% adults on the autism spectrum or with intellectual or psychosocial disability), Phenomena of Interest (interventions in community settings that aimed to improve social, community or civic participation, or capacity to participate), Design (any), Evaluation (any method that evaluated impacts on participation or capacity to participate), and Research type (reviews as journal articles, dissertations or in grey literature, in English, published 2010-2020).

Review Methods

Rapid review methods were used. One researcher screened 27,890 records and 788 potentially eligible full texts. A second reviewer independently screened 20% of records, and ambiguous full text publications. Study quality was extracted, and review quality was assessed with the Assessing Methodological Quality of Systematic Reviews (AMSTAR) checklist. Data from 522 studies in 57 eligible systematic reviews were extracted for narrative synthesis. The Corrected Covered Area (CCA) was calculated to indicate overlap between reviews.

Results

There was a pooled sample of 28,154 study participants, predominantly from studies in North America, the UK and Europe. There was very low overlap between reviews (CCA = 0.3%). Reviews were predominantly low quality: 77.2% of reviews met <50% of AMSTAR criteria. Most studies were low (45.4%) or moderate (38.3%) quality. Three broad intervention categories improved participation, inclusion and belonging outcomes: (1) interventions to help people identify and connect with participation opportunities (e.g., person centred planning); (2) participation opportunities or activities (e.g., joining a community group, sports or outdoor activities, or arts-based activities); and (3) supports to build skills and capacity to participate socially and in the community.

Conclusions

The evidence highlighted that improved social and community participation requires purposeful strategies that identify meaningful participation preferences (e.g., where, when, how, and with whom) and provide support to build capacity or enable ongoing participation. Community capacity building, peer support and advocacy may also be needed to make the community more accessible, and to enable people to exercise genuine choice.
The impact of task workload on cybersickness
Authors: Sepich Nathan C. , Jasper Angelica , Fieffer Stephen , Gilbert Stephen B. , Dorneich Michael C. , Kelly Jonathan W.
Published: August 2022

This study explored the impact of task workload on virtual reality (VR) cybersickness. Cybersickness is a negative side effect of using VR to which many users are susceptible. Previous research on the impact on task workload on cybersickness has yielded no consistent relationships, but given that task workload requires attentional resources, it is worth further investigation of how a demand on attention might increase or decrease cybersickness. In this study, mental workload of participants (N = 151) was modified in three different task groups within the same virtual environment (VE). The Cybersickness Corn Maze VR testbed contained cybersickness-inducing stimuli and tasks with varying workload. The 0-Back group used a controller to select an object as a visual attention task. The 2-Back group performed the 2-Back memory detection task, using a controller to collect objects that matched the object presented two objects ago. The No-Task group passively moved through the environment and was not given a controller. Workload, cybersickness, dropout rate, presence, and task accuracy were compared across groups. Workload was found to be statistically significantly different in each group: highest in the 2-Back group, medium in the 0-Back group, and lowest in the No-Task group, validating the task design. Cybersickness in the 2-Back group was significantly higher than in the No-Task (140% higher) and 0-Back (54% higher) groups measured by the change in simulator sickness questionnaire (SSQ) total severity (Post SSQ - Pre SSQ). The rate of participants’ dropout due to cybersickness was significantly higher in 2-back (33%) as compared with 0-Back (10%), but 0-Back and No-Task dropout rates were not significantly different. These results indicate that 1) task workload affects cybersickness and 2) its effect could be based on a threshold of workload. Presence increased with the addition of a task but plateaued between the 0-Back and 2-Back groups, suggesting that presence can be affected by task workload but only to a certain extent. Task accuracy was shown to negatively correlate with cybersickness within the task groups. A relationship between workload and cybersickness was found and warrants further research into these concepts. This work highlights the need for task workload and attention to be studied as components of the mechanisms underlying cybersickness.
Struma ovarii with contralateral ovarian teratoma: A case report
Authors: Ren Xinjie , Guo Zhaoyou , Bai Jiao
Published: August 2022

Struma ovarii (SO) is a rare form of ovarian teratoma in which the thyroid tissue constitutes over 50% of the tumor. SO comprise 1% of all ovarian tumors. We report the case of a 61-year-old woman who was admitted to the hospital because of frequent urination and dysuria. Abdominal magnetic resonance imaging revealed a mass measuring approximately 16 cm in diameter in the right adnexal area. After transabdominal bilateral adnexectomy, pathological examination revealed a teratoma of the ovary on the right and goiter of the ovary with focal thyroid cancer on the left side. Subsequent total thyroidectomy was performed, and no cancer was found on pathological examination. The patient was treated with thyroxine for a long time after the operation, and there was no recurrence 3 years after diagnosis.
Staged surgery for closed Lisfranc injury with dislocation
Authors: He Wenbao , Xia Jiang , Zhou Haichao , Li Zhendong , Zhao Youguang , Yang Yunfeng , Li Bing
Published: August 2022

Objective

To investigate the clinical efficacy of staged surgery for patients with closed Lisfranc injury and dislocation.

Methods

This study included 48 patients with acute closed Lisfranc injury and dislocation admitted between July 2016 and July 2021. The patients were divided into two groups. 23 patients in group A underwent staged surgeries included emergency reduction within 4–8 h after injury, and open reduction and internal fixation of Lisfranc injury and first tarsometatarsal joint fusion after the swelling had subsided. 25 patients in group B underwent open reduction and internal fixation as an elective procedure after the swelling had subsided. American Orthopedic Foot and Ankle Society (AOFAS) midfoot scores and visual analog scale (VAS) scores were used for assessment at the final follow-up.

Results

A total of 48 patients with closed Lisfranc injury and dislocation were included. The lengths of hospitalization were 11.52 ± 1.61 day and 19.80 ± 2.37 day in groups A and B, respectively. The total lengths of surgery were 67.34 ± 1.71 min and 104.36 ± 8.31 min in groups A and B, respectively. 48 patients completed the final follow-up (follow-up period range: 12–24 months, mean: 18 months). All fractures had healed at 12–18 weeks after surgery (mean: 14.6 weeks). At the 1-year postoperative follow-up, the AOFAS and VAS score was 86.87 ± 4.24 and 1.91 ± 0.78, respectively, during weight-bearing walking in group A patients and 71.72 ± 5.46 and 3.20 ± 1.17 in group B. By the end of the follow-up period, only 2 patients in group B had developed traumatic arthritis and no patients had joint re-dislocation or required secondary surgery.

Conclusion

Staged surgery for closed Lisfranc injury with dislocation reduced the incidence of perioperative complications and achieved good surgical outcomes while shortening the lengths of surgery and hospitalization.