bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel heterozygous PIK3CD mutation presenting with only laboratory markers of combined immunodeficiency
Authors: Mandola Amarilla B. , Dadi Harjit , Reid Brenda , Roifman Chaim M.
Published: May 2020

Introduction: Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Delta (PIK3CD) is one part of a heterodimer forming the enzyme phosphoinositide 3-kinase (PI3K), found primarily in leukocytes. PIK3CD generates phosphatidyl-inositol 3,4,5-trisphosphate (PIP3), and is involved in cell growth, survival, proliferation, motility, and morphology. An increasing number of patients have been described with heterozygous PIK3CD gain-of-function (GOF) mutations, leading to combined immunodeficiency with both B- and T-cell dysfunction. Patients suffer recurrent respiratory infections, often associated with bronchiectasis and ear and sinus damage, as well as severe recurrent or persistent infections by herpesviruses, including EBV-induced lymphoproliferation.

Aim: To present the clinical phenotypic variability of a novel PI3KCD mutation within a family.

Methods: Patient information was collected prospectively and retrospectively from medical records. Comprehensive immune work up, genetic, and signaling evaluation was performed.

Results: We describe here 2 patients, daughter and mother, with heterozygous PIK3CD mutation identified by whole exome sequencing and Sanger confirmation. The child was screen-positive by newborn screening for severe combined immunodeficiency (SCID). Cellular assays revealed an increase in the baseline phosphorylation of T cells in the patient. Furthermore, both patients had hyper-activation of the catalytic domain, resulting in increased phosphorylation of AKT upon activation.

Discussion: GOF mutations affecting the PIK3CD gene are associated with an increased risk for lymphoproliferation leading to Activated PIK3-delta syndrome (APDS). The clinical course of APDS is highly variable, ranging from combined immunodeficiency with recurrent infections, autoimmune complications, and requiring stem cell transplantation, through isolated antibody deficiency, to asymptomatic adults. Our patient is the first to be identified by newborn screening for SCID. Surprisingly, the clinical course has so far been unremarkable, as well, the mother appears to be completely asymptomatic. Nevertheless, the persistent lymphopenia indicates PIK3CD dysfunction. Because of the wide gap between laboratory findings and clinical manifestations, this kindred poses both a diagnostic as well treatment challenge.

Statement of novelty: We report here a novel PIK3CD mutation diagnosed due to abnormal newborn screen for SCID.
Double false-negative traps in urine routine test: a case report
Authors: Qian Xiang , Liang Hanyu , Ren Zhen
Published: May 2020

Background

Urinalysis is one of the most commonly performed tests in clinical practice and supplies important information for a series of clinical conditions, including renal and urinary tract diseases. The clinical laboratory often completes urinalysis through the combined use of urinary dry-chemistry and formed-element analyzers. Urine red blood cell (RBC) morphology test is often used to discriminate the source of hematuria by manual microscopy.

Case presentation

In this case report, we describe a 39-year-old woman with chronic glomerulonephritis (CGN) who underwent both urine routine test and RBC morphology test. Her RBC count was in the normal range and the occult blood test was negative in routine test, while the RBC morphology test indicated the presence of non-homogeneous hematuria.

Conclusions

Therefore, we analyzed the causes of false-negative result on the urine chemical analyzer and the automatic microscope system, respectively.
Evaluation of Soil Coefficients for Two-Parameter Subgrade Soil Model Based on Rigid Plate Loading Tests
Authors: Zhang Wang-Xi , Liu Rui , Yuan Xuan-Xun , Hwang Hyeon-Jong , Yi Wei-Jian
Published: May 2020

Two-parameter elastic foundation models have great potential for approximate soil-foundation-structure interaction analysis. A hurdle to the wide application of this model has been the difficulty of calibrating the two soil coefficients: the compression coefficient k and shear coefficient G. This paper presents a novel parameter calibration method for k and G based on a number of rigid plate loading tests. Empirical k and G values are also recommended. Experiments showed the validity of the proposed method. Two case studies verified the significance of the two-parameter model in the prediction of footing settlement when interactions among neighboring footings exist.
A Novel Apparatus for Dynamic-Static Coupling Tests on Gas-Adsorbed Coal
Authors: Zhang Bing , Wang Hanpeng , Yuan Liang , Li Shucai , Wang Wei , Zhang Chong
Published: May 2020

Laboratory testing is an efficient way to study the deformation and failure mechanisms of outburst coal. However, existing devices can only perform static loading tests on gas-adsorbed coal, which is not necessarily the stress condition encountered in coal seam outburst. Additionally, the traditional testing methods used by existing devices are not conducive to acquiring the dynamic performance of gas-adsorbed coal. In this study, a novel apparatus was developed to visualize gas-solid coupling. The apparatus can perform combined loading corresponding to a “static stress + impact disturbance” and a “static stress + gas pressure relief disturbance” on gas-adsorbed coal. The complex dynamic characteristics of the gas-adsorbed coal are comprehensively monitored by high-speed photography and a self-developed circumferential displacement test device. The effectiveness and reliability of the novel apparatus were confirmed by carrying out mechanical tests on gas-adsorbed coal subjected to a pressure relief disturbance and an impact disturbance. The test results showed that a dynamic disturbance can cause a sudden drop in the bearing capacity, a sudden increase in the volumetric strain, and rapid crack propagation in gas-adsorbed coal. The disturbance can even induce coal burst failure, which is very similar to the outburst phenomenon. The developed scientific apparatus can be used to study the deformation and failure mechanisms of gas-adsorbed coal under combined dynamic and static loading.
Performance Evaluation of SMA Mixtures Modified by Nanoclay
Authors: Ameli Alireza , Norouzi Navid , Jalali Farhang , Khabooshani Mohammad , Babagoli Rezvan , Mamaghani Farzin Poorheydari
Published: May 2020

Fatigue cracking and permanent deformation are two major distresses that occur in asphalt pavements. Several studies in that past have indicated that utilization of nanomaterials, especially nanoclays, has a significant influence on enhancing the performance of pavement. Therefore, the aim of the current paper is to evaluate the influence of montmorillonite nanoclay on intermediate and high-temperature performance of an original binder and mixture modified by styrene-butadiene-styrene (SBS). Different percentages of nanoclay were added to the original binder (1, 2, 3 and 4 % by the weight of the binder). The fatigue and rutting behavior of binders were evaluated through linear amplitude sweep and multiple stress creep recovery (MSCR) tests. To investigate the performance of mixtures, several mixture tests were implemented, such as static and dynamic creep, wheel track, and four-point beam fatigue tests. The results of the MSCR test revealed that the addition of nanoclay and polymer improves the rutting resistance of the binder, and among modified binders, binder modified with 4 % nanoclay and 5 % SBS has the lowest Jnr and highest percent recovery value. Based on the mixture performance tests results, by the addition of nanoclay, the fatigue and rutting resistance of mixtures increased up to 3 %, and by the addition of more nanoclay, a decreasing trend was observed.
RPE Cells Engulf Microvesicles Secreted by Degenerating Rod Photoreceptors
Authors: Ropelewski Philip , Imanishi Yoshikazu
Published: May 2020

Rhodopsin is mislocalized to the inner segment plasma membrane (IS PM) in various blinding disorders including autosomal-dominant retinitis pigmentosa caused by class I rhodopsin mutations. In these disorders, rhodopsin-laden microvesicles are secreted into the extracellular milieu by afflicted photoreceptor cells. Using aXenopus laevismodel expressing class I mutant rhodopsin or Na+/K+-ATPase (NKA) fused to Dendra2, we fluorescently labeled the microvesicles and found retinal pigment epithelial (RPE) cells are capable of engulfing microvesicles containing rhodopsin. A unique sorting mechanism allows class I mutant rhodopsin, but not NKA, to be packaged into the microvesicles. Under normal physiological conditions, NKA is not shed as microvesicles to the extracellular space, but is degraded intracellularly. Those studies provide novel insights into protein homeostasis in the photoreceptor IS PM.
ECE2 regulates neurogenesis and neuronal migration during human cortical development
Authors: Buchsbaum Isabel Y , Kielkowski Pavel , Giorgio Grazia , O'Neill Adam C , Di Giaimo Rossella , Kyrousi Christina , Khattak Shahryar , Sieber Stephan A , Robertson Stephen P , Cappello Silvia
Published: May 2020

During embryonic development, excitatory projection neurons migrate in the cerebral cortex giving rise to organised layers. Periventricular heterotopia (PH) is a group of aetiologically heterogeneous disorders in which a subpopulation of newborn projection neurons fails to initiate their radial migration to the cortex, ultimately resulting in bands or nodules of grey matter lining the lateral ventricles. Although a number of genes have been implicated in its cause, currently they only satisfactorily explain the pathogenesis of the condition for 50% of patients. Novel gene discovery is complicated by the extreme genetic heterogeneity recently described to underlie its cause. Here, we study the neurodevelopmental role of endothelin‐converting enzyme‐2 (ECE2) for which two biallelic variants have been identified in two separate patients with PH. Our results show that manipulation of ECE2 levels in human cerebral organoids and in the developing mouse cortex leads to ectopic localisation of neural progenitors and neurons. We uncover the role of ECE2 in neurogenesis, and mechanistically, we identify its involvement in the generation and secretion of extracellular matrix proteins in addition to cytoskeleton and adhesion.
T2-signal intensity, SSTR expression, and somatostatin analogs efficacy predict response to pasireotide in acromegaly
Authors: Coopmans Eva C , Schneiders Joppe J , El-Sayed Nour , Erler Nicole S , Hofland Leo J , van der Lely Aart-Jan , Petrossians Patrick , Potorac Julia , Muhammad Ammar , Neggers Sebastian J C M M
Published: May 2020

Objective

T2-signal intensity and somatostatin (SST) receptor expression are recognized predictors of therapy response in acromegaly. We investigated the relationship between these predictors and the hormonal and tumoral responses to long-acting pasireotide (PAS-LAR) therapy, which were also compared with responsiveness to first-generation somatostatin receptor ligands (SRLs).

Design

The PAPE study is a cohort study.

Methods

We included 45 acromegaly patients initially receiving SRLs, followed by combination therapy with pegvisomant, and finally PAS-LAR. We assessed tumor volume reduction (≥25% from baseline), IGF-1 levels (expressed as the upper limit of normal), and T2-weighted MRI signal and SST receptor expression of the adenoma.

Results

Patients with significant tumor shrinkage during PAS-LAR showed higher IGF-1 levels during PAS-LAR (mean (S.D.): 1.36 (0.53) vs 0.93 (0.43), P = 0.020), less IGF-1 reduction after first-generation SRLs (mean (S.D.): 0.55 (0.71) vs 1.25 (1.07), P = 0.028), and lower SST2 receptor expression (median (IQR): 2.0 (1.0–6.0) vs 12.0 (7.5–12.0), P = 0.040). Overall, T2-signal intensity ratio was increased compared with baseline (mean (S.D.): 1.39 (0.56) vs 1.25 (0.52), P = 0.017) and a higher T2-signal was associated with lower IGF-1 levels during PAS-LAR (β: −0.29, 95% CI: −0.56 to −0.01, P = 0.045). A subset of PAS-LAR treated patients with increased T2-signal intensity achieved greater reduction of IGF-1 (mean (S.D.): 0.80 (0.60) vs 0.45 (0.39), P = 0.016).

Conclusions

Patients unresponsive to SRLs with a lower SST2 receptor expression are more prone to achieve tumor shrinkage during PAS-LAR. Surprisingly, tumor shrinkage is not accompanied by a biochemical response, which is accompanied with a higher T2-signal intensity.
Multiple QTL Mapping in Autopolyploids: A Random-Effect Model Approach with Application in a Hexaploid Sweetpotato Full-Sib Population
Authors: da Silva Pereira Guilherme , Gemenet Dorcus C , Mollinari Marcelo , Olukolu Bode A , Wood Joshua C , Diaz Federico , Mosquera Veronica , Gruneberg Wolfgang J , Khan Awais , Buell C Robin , Yencho G Craig , Zeng Zhao-Bang
Published: May 2020

Genetic analysis in autopolyploids is a very complicated subject due to the enormous number of genotypes at a locus that needs to be considered. For instance, the number of...

In developing countries, the sweetpotato, Ipomoea batatas (L.) Lam. (2n=6x=90), is an important autopolyploid species, both socially and economically. However, quantitative trait loci (QTL) mapping has remained limited due to its genetic complexity. Current fixed-effect models can fit only a single QTL and are generally hard to interpret. Here, we report the use of a random-effect model approach to map multiple QTL based on score statistics in a sweetpotato biparental population (‘Beauregard’ × ‘Tanzania’) with 315 full-sibs. Phenotypic data were collected for eight yield component traits in six environments in Peru, and jointly adjusted means were obtained using mixed-effect models. An integrated linkage map consisting of 30,684 markers distributed along 15 linkage groups (LGs) was used to obtain the genotype conditional probabilities of putative QTL at every centiMorgan position. Multiple interval mapping was performed using our R package QTLpoly and detected a total of 13 QTL, ranging from none to four QTL per trait, which explained up to 55% of the total variance. Some regions, such as those on LGs 3 and 15, were consistently detected among root number and yield traits, and provided a basis for candidate gene search. In addition, some QTL were found to affect commercial and noncommercial root traits distinctly. Further best linear unbiased predictions were decomposed into additive allele effects and were used to compute multiple QTL-based breeding values for selection. Together with quantitative genotyping and its appropriate usage in linkage analyses, this QTL mapping methodology will facilitate the use of genomic tools in sweetpotato breeding as well as in other autopolyploids.
Pathways for Improving Inpatient Pediatric Asthma Care (PIPA): A Multicenter, National Study
Authors: Kaiser Sunitha V. , Jennings Brittany , Rodean Jonathan , Cabana Michael D. , Garber Matthew D. , Ralston Shawn L. , Fassl Bernhard , Quinonez Ricardo , Mendoza Joanne C. , McCulloch Charles E. , Parikh Kavita
Published: May 2020

BACKGROUND AND OBJECTIVES:

Pathways guide clinicians through evidence-based care of specific conditions. Pathways have been demonstrated to improve inpatient asthma care but mainly in studies at large, tertiary children’s hospitals. It remains unclear if these effects are generalizable across diverse hospital settings. Our objective was to improve inpatient asthma care by implementing pathways in a diverse, national sample of hospitals.

METHODS:

We used a learning collaborative model. Pathway implementation strategies included local champions, external facilitators and/or mentors, educational seminars, quality improvement methods, and audit and feedback. Outcomes included length of stay (LOS) (primary), early administration of metered-dose inhalers, screening for secondhand tobacco exposure and referral to cessation resources, and 7-day hospital readmissions or emergency revisits (balancing). Hospitals reviewed a sample of up to 20 charts per month of children ages 2 to 17 years who were admitted with a primary diagnosis of asthma (12 months before and 15 months after implementation). Analyses were done by using multilevel regression models with an interrupted time series approach, adjusting for patient characteristics.

RESULTS:

Eighty-five hospitals enrolled (40 children’s and 45 community); 68 (80%) completed the study (n = 12 013 admissions). Pathways were associated with increases in early administration of metered-dose inhalers (odds ratio: 1.18; 95% confidence interval [CI]: 1.14–1.22) and referral to smoking cessation resources (odds ratio: 1.93; 95% CI: 1.27–2.91) but no statistically significant changes in other outcomes, including LOS (rate ratio: 1.00; 95% CI: 0.96–1.06). Most hospitals (65%) improved in at least 1 outcome.

CONCLUSIONS:

Pathways did not significantly impact LOS but did improve quality of asthma care for children in a diverse, national group of hospitals.
Fertilización con nitrógeno, fósforo, potasio y calcio en mora (Rubus glaucus Benth.): Efecto sobre Antracnosis bajo condiciones controladas
Authors: Bautista Monetalegre Luis Gabriel , Bolaños Benavides Martha Marina , Fischer Gerhard , Argüelles Cárdenas Jorge Humberto
Published: May 2020

Para buscar alternativas de manejo de la antracnosis causada por el hongo Colletotrichum gloeosporioides en el cultivo de mora (Rubus glaucus Benth.), en el Centro de Investigación Tibaitatá de la Corporación Colombiana de Investigación Agropecuaria AGROSAVIA (antes CORPOICA), se realizó un experimento con el objetivo de evaluar el efecto de la aplicación de los elementos mayores nitrógeno (N), fósforo (P), potasio (K) y calcio (Ca) sobre infecciones del hongo C. gloeosporioides cepa-52. Para el efecto se utilizó un diseño de bloques completos al azar con un arreglo de tratamientos en un diseño compuesto central ortogonal. Para evaluar la relación de los niveles de fertilización y la severidad de la enfermedad, se hizo una inoculación artificial en tallos de mora de Castilla con espinas, mediante discos de micelio de 0.5 cm a una concentración de 9.53 x 104 conidias. Las observaciones consistieron en: severidad de la enfermedad (S), periodo de incubación (PI) y tasa de desarrollo (r). Los análisis de datos se hicieron por el método de conglomerados sobre la variable severidad, un análisis de correlación de Pearson entre variables, así como una regresión para estimar el efecto de los nutrientes aplicados sobre la severidad de la cepa-52 de C. gloeosporioides. Los tratamientos se concentraron en cuatro grupos con los rangos (entre paréntesis) S (15.9% y 91.8%), PI (9 y 15.3) y r (0.0254 y 0.0468). Se observó correlación positiva y significativa entre S y r (P < 0.001) y negativa entre PI con S y r (P < 0.001). Mediante análisis de regresión se generó un modelo lineal que permitió comprobar la reducción de la severidad de la enfermedad con el incremento de la dosis de N y un incremento con los niveles de P y Ca aplicados.
Electrochemical quantification of chlorpyrifos in white (Allium cepa L.) and green (Allium fistulosum L.) onions Cuantificación voltamétrica de clorpirifos en cebolla de bulbo (Allium cepa L.) y cebolla de rama (Allium fistulosum L.)
Authors: García Colmenares José Mauricio , Páez Cepeda Leidy Paola , Chaparro Sandra Patricia
Published: May 2020

The Linear sweep voltammetry method (LSP) of a common pesticide such as chlorpyrifos (CP) an organophosphate pesticide (OPP) in white (Allium cepa L.) and green onions (Allium fistulosum L.) was investigated. A hanging mercury drop electrode (HMDE) Ag/AgCl was used as reference electrode and platinum as a counter electrode. The OPP was extracted from the onion through liquid-liquid partition using acetone/dichloromethane as the solvent. Validated method was compared with GC/ECD as a reference method. CP concentration was not different between the two analytical techniques; the analysis was performed with a confidence level of 90%, corresponding to the polarographic method. CP concentration was 0.8400 ± 0.062 ppm in Allium cepa and 0.062 ± 0.300 ppm in Allium fistulosum L. These values exceed the ADI and ARD values for chlorpyrifos established by current regulations.
Efecto de enmiendas agrícolas en las reservas de carbono y nitrógeno en Oxisoles de la Amazonía del Brasil
Authors: Sacramento José Augusto Amorim Silva do , Gomes Paulo Henrique Da Silva , Veloso Carlos Alberto Costa , Silva Arystides Resende , Dantas Edilândia Farias , Fajardo Juan Daniel Villacis
Published: May 2020

En un Oxisol Distrófico de textura arcillosa del campo experimental de la Empresa Brasileña de Producción Agropecuaria (Embrapa) en la ciudad de Belterra, estado de Pará (Brasil) se evalúo el efecto de los correctivos agrícolas (cal dolomita y yeso) en las reservas de carbono y nitrógeno en el suelo. Las muestras de suelo fueron tomadas en pequeñas calicatas de 0.60 x 0.60 x 0.60 en tres repeticiones y un diseño en bloques al azar con cinco tratamientos (cal/yeso -kg/ha) en sistemas agrícolas durante 5 años en rotación de maíz/soya bajo siembra directa (SSD). Los tratamientos (T), cal (GO)/yeso (GO), fueron: T1 (C0G0) = sin cal/sin yeso, T2 (C1G1) = 1000/500, T3 (C4G2) = 4000/1000, T4 (C4G2) = (4000/1000), más un testigo en bosque nativo (BNA) sin enmiendas. Las muestras fueron tomadas cada 10 cm hasta 60 cm de profundidad en el suelo. Los resultados mostraron que la aplicación de yeso más cal en sistema de siembra directa de cultivos comerciales maíz/soya aumenta la disponibilidad de nutrientes en las capas superiores del suelo hasta 20 cm de profundidad. Los mejores resultados en el perfil del suelo se obtuvieron con el tratamiento C4G2.
Effects of vitamin pre-sowing treatment on sweet maize seedlings irrigated with saline water
Authors: Pradi Vendruscolo Eduardo , Seleguini Alexsander
Published: May 2020

Salinity stress represents an obstacle for the production of plants of commercial interest, including sweet maize.  Among the techniques used to suppress the effects caused by excess salts in the soil is the exogenous application of vitamins.  Thus, the objective of this study was to evaluate the effect of the pre-sowing treatment of sweet maize seeds in solutions of thiamine, niacin and these two vitamins combined on the development of the plants irrigated with saline water.  The treatments were composed by the 24 h pre-sowing treatment with water, thiamine solution (thiamine 100 mg L-1), niacin solution (niacin 100 mg L-1) and a combined solution with these two vitamins (thiamine 50 mg L-1 + niacin 50 mg L-1) irrigated with saline water.  A control (pre-sowing treatment with water and irrigation with non-saline water) was also used.  It was observed that salinity stress affects the sweet maize initial development. Application of isolate thiamine or niacin and its combined application improves the relative contents of chlorophyll ‘a’ and total, height of shoot, leaf number, diameter of stem and shoot, root and total dry matter, reducing dry matter losses by 8.89%, 25.46% and 39.60%, respectively.  Soaking seeds for 24 h in thiamine and niacin solutions improve the initial growth of sweet maize plants under salt stress and a combined vitamin solution (thiamine 50 mg L-1 + niacin 50 mg L-1) effectively reduces the salt stress negative effect on the initial development of these plants.
Effects of humus and shading levels in the production of Lactuca canadensis L. seedlings
Authors: Pallaoro Dryelle Sifuentes , Arantes Cárita Rodrigues de Aquino , Correa Amanda Ribeiro , Camili Elisangela Clarete , Coelho Maria de Fátima Barbosa
Published: May 2020

The objective of this study was to verify levels of shade and doses of earthworm humus in the production of Lactuca canadensis L. seedlings.  The experimental design was completely randomized in a 4 x 5 factorial scheme (four levels of shade and five doses of humus).  The different levels were obtained using black polyethylene screen with 35, 50 and 70% shade and in full sun. The substrates were obtained by mixing commercial substrate for vegetables (Vivatto Slim ® plus), with earthworm humus in the proportions of 0, 15, 30, 45 and 60%.  There was no seedlings emergence in the full sun and, the treatment with 50% of shade allows the best development. The substrate without humus and with 60% presented superior results but the higher dose stood out for number of leaves (3.14) and chlorophyll content (32.9%), in 50% of shade. For the other variables, there was no difference between the commercial substrate and 60% of humus, so the commercial substrate can be used in the production of Lactuca canadensis L. seedlings, considering the ratio benefit/cost ratio.
Caracterización por colorimetría de accesiones de plátano del Sistema de Bancos de Germoplasma en Colombia
Authors: Enriquez Valencia Ayda Lilia , Ordoñez Santos Luis Eduardo , Caicedo Arana Alvaro , Rodriguez Henao Eberto
Published: May 2020

El color es uno de los atributos sensoriales que mejor define la calidad de un alimento, no obstante las investigaciones sobre el color superficial de pulpa de frutos de musáceas en Colombia son escasas. Esta información es de gran utilidad para identificar materiales promisorios destinados para consumo fresco y procesamiento agroindustrial. En este estudio se caracterizó el color superficial de la pulpa de 25 accesiones de plátano Musa sp. (Musaceae) del Banco de Germoplasma de Musáceas del Sistema de Bancos de Germoplasma de la Nación para la Alimentación y la Agricultura (SBGNAA) existente en la Corporación Colombiana de Investigación Agropecuaria (Agrosavia) en Palmira, Colombia. Las coordenadas CIEL*a*b* fueron determinadas por triplicado utilizando un colorímetro, entre ellas se calcularon parámetros de cromaticidad (C*), tonalidad (hº) y diferencia de color (ΔE). Las accesiones de plátano presentaron rangos de L*(17.31-79.30), a*(8.73-30.64). y b*(15.24-95.49), C*(17.77-98.22), hº(38.24-81.00) y ΔE*(147.07-6851.24). Las pulpas de plátano fueron clasificadas colorimétricamente entre amarillos tenues a amarillos intensos. La variación de las coordenadas colorimétricas a*, hº, y ΔΕ se ajustaron a una cinética de orden cero; y L*, b* y C* describieron una cinética de primer orden. Las accesiones Benedetta, Cachaco espermo, Cachaco sin bellota, Dominico mocho, Dominico mutante, GAEP-2, Hartón tigre y Maia Maoli Risaralda, fueron clasificadas como materiales promisorios para consumo en fresco o procesos agroindustriales, por los bajos valores de la constante de velocidad cinética (k) registrados en el presente estudio.
Effect of Bacillus subtilis and Bacillus amyloliquefaciens culture on the growth and yield of off-season potato (Solanum tuberosum L.)
Authors: Kantar Faik , Uysal Aysel
Published: May 2020

A study was carried out in order to investigate the effect of microbial fertilizer application on off-season potato (Solanum tuberosum L. cv. Universia) under field conditions in early spring and late autumn in 2016.  The experiment included Control (C, no application), Standard Fertilizer Application (SFA), Microbial Fertilizer Application (MFA) and SFA+MFA  treatments.  An equal mixture of three strains of Bacillus subtilis VKPM B-10641(DSM 24613), Bacillus amyloliquefaciens VKPM B-10642 (DSM 24614) and Bacillus amyloliquefaciens В-10643 (DSM 24615) was applied at 1x109 cfu/ml to tubers before planting and to plants at flowering stage in MFA and SFA+MFA plots.  Treatments affected the plant growth parameters.  SFA plots produced the highest tuber yields in both seasons.  MFA plots had earlier emergence time (40 days), higher number of stems per plant (3), higher number of leaves per plant (37.2), thicker stem diameter (10.15 mm), higher above ground biomass yields (5.42 t/ha), higher single tuber weight (104.21 g) and higher tuber yields (22.06 t/ha) compared with control plots (42 days, 2.2, 31.2, 9.15 mm, 4.40 t/ha, 90.88 g and 20.14 t/ha respectively).  Tuber yield  in MFA plots (26.56 t/ha) was equal to SFA plots (26.81 t/ha) in warmer autumn planting. Combination of SFA and MFA produced lower tuber yields (21.72 t/ha) than SFA treatment (26.81 t/ha).  In conclusion, Bacillus subtilis and Bacillus amyloliquefaciens gave comparable tuber yields to chemical fertilizer application in warmer autumn plantings, but lower tuber yields in colder spring conditions warranting further experiments with  cold tolerant psychrophilic bacterial strains for off-season potato production.
Plant growth and yield of butter kale (Brassica oleracea L. var. acephala), as influenced by the combined application of bovine manure and rock powder
Authors: Micaela Micaela B. Pereira , Dias Thiago Jardelino , Neriane Neriane Rodrigues de Lima , Evandra Evandra Da Silva Justino , Daivyd Daivyd S. Oliveira , Mario Mario L. Martins Veras
Published: May 2020

The supply of organic matter in soil of arid and semiarid in Brazilian regions is very import, as it allows to increase crop yields.  However, there are few studies on alternative sources of fertilizer for vegetables.  Therefore, the objective was to evaluate the effect of different doses of bovine manure and rock powder (RP) on growth characteristics, concentration of chlorophyll, and physiological indices and production of (B. oleracea L. var. acephala).  The treatments were arranged in five randomized blocks in a factorial 4 x 4 reference doses of bovine manure (60, 120, 180 and 240 g pit-1) combined with doses of rock powder (6, 12, 18 and 24 g pit-1).  Doses of bovine manure and rock powder of 180 and 18 g, respectively, increased the growth of B. oleracea by plant height, stem diameter, leaf number.  The combination doses of bovine manure 120 g + 12 g rock powder and 120 g of bovine manure + 18 g rock powder, provided, in this order, larger leaf area results for the productivity of B. oleracea.  The bovine manure promotes greater concentration of CO2, net photosynthesis and stomatal conductance of B. oleracea plant, while rock powder limits these characteristics.  Intermediate doses of bovine manure and RP promote higher growth and yield of B. oleracea.
Efecto de la procedencia sobre el comportamiento productivo de Prosopis alba en plantación
Authors: Fontana María Laura , Pérez Víctor Ramón , Luna Claudia Verónica
Published: May 2020

Prosopis alba (algarrobo blanco) es una especie forestal nativa utilizada principalmente para aserrío en Argentina. La Ley 27.487/19 de fomento forestal considera las densidades y prácticas adecuadas para el logro de madera de calidad. Además del buen manejo, la correcta selección del origen del material genético es una estrategia importante para la obtención de buenos rendimientos de madera. Con el objeto de evaluar el comportamiento de plantas de P. alba de tres procedencias se establecieron ensayos en las localidades de Corrientes y Chaco (Argentina). Se trabajó con plantines logrados a partir de semillas de las procedencias Santiagueña, Chaqueña y Salta Norte. Una vez establecidos en campo se evaluó el índice de respuesta neta y cuatro variables dasométricas (altura total, diámetro a la altura del cuello, volumen de árbol en pie e incremento corriente anual). No se detectó efecto del origen geográfico de las plantas sobre la sobrevivencia. Considerando los parámetros dasométricos estudiados, la procedencia Salta Norte resultó superior respecto a las procedencias Chaqueña y Santiagueña. En Sáenz Peña (Chaco) todas las variables dasométricas evaluadas resultaron estadísticamente superiores, mientras que en Corrientes solamente el diámetro a la altura del cuello y altura total fueron superiores. Se observó que la procedencia Salta Norte se destaca como la de mejor comportamiento en ambas localidades. Por tanto, se concluye que la procedencia del germoplasma de P. alba influye en su comportamiento productivo.
Electrophoretic behavior of ewe milk proteins from local breeds Rembi and Ouled-Djellal of the Algerian central steppe
Authors: Illoul Assia Zobiri , Yabrir Benalia , Laoun Abbes , Mati Abderrahmane
Published: May 2020

In order to characterize the production of sheep milk in Algeria (North of Africa) and to detect molecular markers related to the constitution of the protein phase of this milk, we proposed to analyze the electrophoretic behaviors of caseins and serum proteins under various migration conditions (native, urea and SDS-PAGE), from milk collected during the first three months of the year, from two breeds Ouled-Djellal and Rembi, living in the central area steppe. The profiles obtained show a great similarity and homogeneity between the different samples of the milk of the two breeds of ewes studied as to the number and intensity of the revealed migration bands. Some of the latter are nevertheless distinguished from cow’s milk by different levels of migration and intensity, which require partial sequencing to be able to identify them with certainty.
Epistemologías otras en la investigación en diseño. Transformaciones para el diseño inclusivo
Authors: Rodríguez Cely Diana Milena , Ospina Salazar Ana Maria
Published: May 2020

Derivado de un ejercicio de reflexión se propone a través de este artículo evidenciar cómo las epistemologías otras provenientes de la experiencia de la discapacidad, pueden llegar a nutrir las metodologías participativas y la investigación en diseño. Centrado en una perspectiva desde el conocimiento situado y el diseño encarnado atravesado por las diferentes formas de investigar en diseño, se busca mostrar una mirada del diseño en el que éstas nuevas apuestas epistemológicas puedan ser posibles y que al mismo tiempo puedan funcionar como una herramienta de transformación en el marco del diseño inclusivo. Finalmente, de esto se concluye que este acercamiento epistémico a otras formas de ser y estar en el mundo desde el diseño, puede llegar tanto a igualar los principios de participación de los sujetos en la práctica del diseño, como a impactar las relaciones entre diseñador – usuario para que éstas se tornen más equitativas y paritarias, así como una forma de visibilizar y valorar nuevas formas de ser y estar en el mundo.
La bitácora de diseño, artefacto cognitivo de aprendizaje. Externalización de modelos mentales y metacognición
Authors: Gonzalez-Tobon Juanita , Cuervo Roberto , Hernández Mihajlovic Edgar , Camacho Jorge
Published: May 2020

La bitácora, un instrumento utilizado en los procesos de enseñanza-aprendizaje en diseño, suele utilizarse como registro del proyecto y no como un medio de externalización de los modelos mentales de los estudiantes y su transformación durante el curso de una asignatura. En este artículo de reflexión teórica se indaga por cómo la bitácora de diseño puede ser un artefacto cognitivo de aprendizaje para promover la metacognición en el proceso formativo. Para ello, se propone una aproximación cognitivista histórico-cultural en relación con el concepto de metacognición, como un aspecto fundamental para que se dé el aprendizaje en  profundidad y comprender así la bitácora de diseño desde la noción de artefacto cognitivo, la cual se construye en las relaciones entre los agentes involucrados en el aula. Se establece el marco conceptual desde donde se formulan las categorías y posibles variables para comprender las representaciones externas que se presentan en la bitácora de diseño: sus niveles físico, simbólico y cognitivo. Las representaciones externas, como elementos simbólicos, son evidencia de los modelos mentales del sujeto y su aproximación a la solución de problemas desde el pensamiento diseñístico. Como resultado, se presenta una matriz con rasgos observables de las dimensiones y procesos metacognitivos.
¿Cómo evaluar la cognición creativa al enseñar diseño industrial? Un insumo para su aprendizaje
Authors: Cuervo Pulido Roberto , Hernández Mihajlovic Edgar
Published: May 2020

Evaluar la creatividad en diseño industrial suele centrarse en los resultados artefactuales más que en las habilidades cognitivas que la soportan, reduciendo el enfoque evaluativo a una dimensión instrumental. Esto puede suceder porque la cognición es un proceso discreto, que ocurre en la mente del sujeto sin ser observable de manera directa, a diferencia del resultado artefactual final. De ahí la pregunta por cómo evaluar las habilidades de la cognición creativa en los procesos de enseñanza-aprendizaje del diseño enmarcada en la evaluación para el aprendizaje. Para dar respuesta a ello se formuló un modelo de evaluación para favorecer la enseñanza de las habilidades de la cognición creativa en los procesos proyectuales en diseño industrial, no solo desde un enfoque psicométrico, sino como insumo para gestionar los procesos de enseñanza-aprendizaje. El modelo midió cuatro variables propias del pensamiento divergente: fluidez, flexibilidad, originalidad y elaboración en las dos fases del proceso proyectual de diseño: problematización e ideación. Este ejercicio visibilizó algunas habilidades cognitivas creativas mediante el diseño de instrumentos de medición e interpretación de las variables, lo que permitió que estudiantes y profesores conocieran su nivel de desarrollo, haciendo consciencia sobre dichas habilidades aplicadas al proyecto de diseño industrial.
Using prototypes to produce high-resolution systemic future maps. A proposed model for design research and knowledge
Authors: De la Rosa Munar Juan Alfonso , Ruecker Stan
Published: May 2020

Design’s arguments of innovative transformation and its constant search for a preferred future have become a contemporary principle of the discipline, and yet most design models limit their process to the production of the next stage of incremental innovation. This approach to the future carries significant systemic problems that can go from unexpected behavioral changes to unintended discrimination against certain groups, especially when addressing complex social problems and transformations. Avoiding these systemic problems might require the use of Design Research to study the conditions that produced them. However, design researchers seem to still disagree on the nature of Design Research, and the specific knowledge that can be produced through it. This paper seeks to introduce a possible model for design research that integrates various design theories to help obtain a more sophisticated view of the systemic situation of possible preferred futures. The goal of these process is to seek to produce a better understanding of how stakeholders envision their future, their intentions, values and needs as a systemic view within any given socio-technical system.
Justicia social y diseño
Authors: Sarmiento Pelayo Martha Patricia , Fernandez Moreno Carmen Aleida
Published: May 2020

Este artículo vincula la justicia social con el diseño. Para iniciar, presenta algunas de las definiciones más aceptadas sobre la justicia. Posteriormente, retoma los planteamientos de la justicia con equidad de John Rawls y los aportes del enfoque de capacidades de Martha Nussbaum; ambos autores encuadran sus ideas en la justicia distributiva. Desde la tradición crítica, se presenta la apuesta de justicia como reconocimiento de Axel Honneth. Dialogando con estas dos perspectivas, que nutren la actual concepción de justicia social, se propone cómo el diseño contribuye a alcanzarla.
Creatividad situada como metáfora de inclusión
Authors: Herrera Castiblanco Samuel Alberto
Published: May 2020

En este artículo se presentan algunos planteamientos sobre creatividad situada que constituyen puentes de reflexión inclusiva de las lógicas cotidianas experimentadas por una mipyme que produce zapatos drag queen en el barrio Restrepo de Bogotá (Colombia). Constituye un ejercicio de comprensión, a partir de las acciones que sus actores realizan para visibilizar su mundo creativo. Este artículo establece planos de discusión a partir de las dinámicas derivadas de la entre la creatividad y la inclusión.
The Pharmacological Evaluation of Flax Seed Oil
Authors: Duran Derya
Published: May 2020

Flax seed oil is important food and nutrition sources. It has a low content of saturated fatty acids (9% of all the fatty acids), a moderate content of single saturated fatty acids (about 18%) and a high content of unsaturated fatty acids (about 73%). This oil can be used as a nutritional supplement and is significant in respect of containing omega-3 fatty acid, especially a high level of ALA and a low level of omega-6 fatty acids. The protein content of flax seed varies between 20-30%, and it contains a high level of globulin (linin and conlinin) and gluten. The proportion of non-protein nitrogen constitutes 21.7% of the total nitrogen content. The total nitrogen content has been reported as 3.25g/100 grams of seed. Today, flax seed stands out among the functional additives in the food industry due to the presence of α-linolenic acid, lignans and fibers in its content. Among the oils obtained from seeds, flax seed comes into prominence since it has high contents of α-linoleic acid (ALA, 18:3n-3) and lignans. Flax seed contains oil by 35-45%, and 45-52% of it consists of ALA. ALA is classified as omega-3 group fatty acid and has anti-inflammatory, antithrombotic and antiarrhythmic characteristics.
Lexicalising phonological structure in morphemes
Authors: Nasukawa Kuniya
Published: May 2020

By comparing different theoretical models of phonological representation, this paper considers (i) what kinds of properties are lexically specified in morpheme-internal phonological structure, and (ii) how this morpheme-internal phonological structure is constructed before being stored in the mental lexicon. The aim is to contribute to the ongoing development of a model which can characterize the lexicalisation of phonological structure within morphemes.
SO SÁNH MỘT SỐ ĐẶC ĐIỂM ĐỘNG LỰC VÀ TRẦM TÍCH VEN BỜ CHÂU THỔ SÔNG HỒNG VÀ DƯƠNG TỬ
Authors: Vinh Vu Duy , Hai Nguyen Minh
Published: May 2020

The Red river delta coastal area (Vietnam) and Yangtze river delta coastal area (China) not only play an important role in developmental process of each country but also show typical characteristics of hydrodynamics and sediment dynamics of estuaries and delta coastal areas. Based on previous studied results of hydro-sediment dynamics in two delta coastal zones that were published, this paper gives a comparison of hydrodynamics and sediment dynamics of the coastal zones of Red river and Yangtze river. The results showed that there are some similar features of these two regions such as riverine hydrology, decreased fluvial sediment flux due to the dam, grain size of suspended sediment, alongshore sediment and morphological change. Besides, these two regions also have some distinct characteristics such as tidal regime and residual field currents.
Some features of marine dynamics caused by damrey typhoon (11/2017) and its impacts on coral reef in Ninh Van waters, Khanh Hoa province
Authors: Long Bui Hong , Chung Tran Van , Thu Phan Minh , Y. Latupov
Published: May 2020

Typhoon Damrey (Vietnamese name is Typhoon No.12 in 2017) landed in South Phu Yen - North Khanh Hoa provinces in the morning of 4th Nov 2017 causing severe damage to people, material and serious consequences on the social and economy of the region (especially Khanh Hoa Province). So far, there have been no published papers and reports on the results of analysis of observing and monitoring data, calculations on marine dynamics as well as their impact on some marine ecosystems. Based on the results of coral reef survey in the study area before and after storm as well as hydrodynamic model results, the paper presents some results of data analysis of monitoring water level, calculations of wave field, currents caused by Damrey Typhoon in coastal waters of Khanh Hoa Province. On that basis, there have initial comments on the impact of storm on the coral reef of this region for management, forecasting and mitigation of local natural disaster damage in the future.
Assessment of soil and soil-water salinity in Ben Tre province by electromagnetic technology
Authors: Thanh Le Ngoc , Dung Nguyen Quang , Tung Luu Hai
Published: May 2020

Assessment of soil and soil-water salinity is essential in agricultural production, therefore it is necessary to find out the non-costly, effective, rapid and reliable integrated methodology for this purpose. The paper presents the results of using the electromagnetic induction instrument EM31-MK2Ô in combination with collecting and analyzing soil and soil-water samples, and applying GIS and geostatistical techniques to assess the current status of soil and soil-water salinity in Ben Tre province. Apparent soil electrical conductivity ECa measured from ground surface to 6 m in depth increases from inland to the sea in northwest - southeast direction; ECa is closely related to topsoil salinity to 30 cm deep and to soil-water salinity at depth of 10–100 cm. Current status of soil and soil-water salinity in 2018 was assessed with a 4-fold increase in information, from 16 km2/data point to 4 km2/data point. Consequently four maps were established, consisting of electrical conductivity ECe and total solube salt TSS distributions of soil; electrical conductivity σw and total dissolved solid TDS distributions of soil-water.
DNA barcoding for identification of some fish species (Carangidae) in Vietnam coastal area
Authors: Thu Pham The , Linh Nguyen Manh , Quan Nguyen Van , Chien Pham Van , Ly Dao Huong , Hiep Le Ba Hoang
Published: May 2020

Carangidae family has got about 148 species belonging to 32 genera. In Vietnam, Carangidae is of high commercial value and playing an important role in the ecosystem. In the context Vietnam has received yellow card for seafood since Nov. 2017 by the EU, in which one of the main reasons was related to the restriction of traceability. In this study, DNA barcoding technique of mitochondrial cytochrome oxidase I (COI) gene was used to classify 56 specimens of Carangidae from three coastal areas (Northern, Central and Southern) in Vietnam to evaluate the effectiveness compared to the morphological classification method. Results showed that 21 species belonging to 16 genera were determined by the COI barcode while 18 species (16 genera) were determined when using morphological method. Seriola quinqueradiata and Trachinotus anak were newly recorded in Vietnam. From 56 sequences with 660 bp of mtDNA (COI), total 27 haplotypes were detected; haplotype diversity (h) and nucleotide diversity (π) were 0.903 ± 0.00060 and 0.14%, respectively. The DNA barcodes of COI gene of 21 species in Carangidae which were developed in this study could be used as a basis for comparison and traceability of their products. In addition, the results showed the high potentiality in using COI barcode to identify Carangidae fish in Vietnam.
Threadfin bream (Nemipteridae) resources in the sea of Vietnam based on the bottom trawl surveys
Authors: Anh Tran Nhat , Cuong Tran Van
Published: May 2020

The threadfin bream (Nemipteridae) resources in the sea of Vietnam were analysed using the results of  bottom trawl surveys conducted by the assessment of living marine resources project in Vietnam (ALMRV) in period 2000–2005 and comprehensive investigation of marine fisheries resources project in Vietnam sea during 2012–2013. Results showed that a total of 23 species belonging to 4 genera of Nemipterus, Parascolopsis, Pentapodus and Scolopsis were recorded. The Southeast region has the highest number of species encountered with 26 species, and the lowest number in Central waters with 16 species. The highest number of species was encountered at 50–100 m depth range with 25 species and the lowest number at the depth of less than 20 m with 9 species. Catch of threadfin bream comprised more than 1% of total catch of surveys. In threadfin bream family, genus Nemipterus was the most abundant, the catch of this genus contributed from 75.1% to 88.4% of the family catch. The catch per unit effort decreased from Northern region to Southern region, while the catch in the Northeast monsoon was higher than in the Southwest monsoon. The biomass of threadfin bream in Vietnamese water was estimated to be 7,534 to 12,229 tons depending on the monsoon patterns.