bluuebunny/crossref_metadata_2025 huggingface dataset:
Mosaic Loss of Chromosome Y Is Associated With Functional Outcome After Ischemic Stroke
Authors: Dorvall Malin , Pedersen Annie , Dumanski Jan P. , Söderholm Martin , Lindgren Arne G. , Stanne Tara M. , Jern Christina
Published: July 2023

BACKGROUND:

Mosaic loss of chromosome Y (LOY) is associated with cardiovascular and neurodegenerative diseases in men, and genetic predisposition to LOY is associated with poor poststroke outcome. We, therefore, tested the hypothesis that LOY itself is associated with functional outcome after ischemic stroke.

METHODS:

The study comprised male patients with ischemic stroke from the cohort studies SAHLSIS2 (Sahlgrenska Academy Study on Ischemic Stroke Phase 2; n=588) and LSR (Lund Stroke Register; n=735). We used binary logistic regression to analyze associations between LOY, determined by DNA microarray intensity data, and poor 3-month functional outcome (modified Rankin Scale score, >2) in each cohort separately and combined. Patients who received recanalization therapy were excluded from sensitivity analyses.

RESULTS:

LOY was associated with about 2.5-fold increased risk of poor outcome in univariable analyses (
P
<0.001). This association withstood separate adjustment for stroke severity and diabetes in both cohorts but not age. In sensitivity analyses restricted to the nonrecanalization group (n=987 in the combined cohort), the association was significant also after separate adjustment for age (odds ratio, 1.6 [95% CI, 1.1–2.4]) and when additionally adjusting for stroke severity and diabetes (odds ratio, 1.6 [95% CI, 1.1–2.5]).

CONCLUSIONS:

We observed an association between LOY and poor outcome after ischemic stroke in patients not receiving recanalization therapy. Future studies on LOY and other somatic genetic alterations in larger stroke cohorts are warranted.
Structural Stiffness and Mechanical Analysis of Fiber Wound Composite Sports Equipment Reinforced with Carbon Fiber Materials
Authors: Guo Jian
Published: July 2023

Continuous improvements in sports equipment are crucial for enhancing performance and ensuring safety. Carbon fiber composites, comprising carbon fibers and a matrix such as resin, metal, or ceramics, have exceptional strength and stiffness properties. This study focused on the application
of carbon fiber reinforced composites in sports equipment, with an emphasis on optimizing the stiffness of lightweight equipment. The research revealed that fiber-wound composites exhibit nonlinear behavior, and the overlapping fiber structure can impede damage propagation, leading to greater
in-plane shear failure strain compared to composite laminates. The study aimed to predict the mechanical behavior and structural stiffness of fiber-wound composite sports equipment reinforced with carbon fibers. The findings can be used to improve the design and manufacturing process of sports
equipment, leading to better performance and durability. The utilization of these materials in sports equipment may also contribute to reducing the weight of the equipment, thereby enhancing athlete’s performance and reducing the risk of injury.
Early cessation of calcineurin inhibitors is feasible post–haploidentical blood stem cell transplant: the ANZHIT 1 study
Authors: Moore John , Hamad Nada , Gottlieb David , Bajel Ashish , Ritchie David , Yeung David , Greenwood Matthew , Purtill Duncan , Tran Steven , Solterbeck Annie , Aarons Donna , Kwan John
Published: July 2023

Haploidentical hematopoietic stem cell transplant (haplo-HSCT) using posttransplant cyclophosphamide (PTCy) is appropriate for those who lack matched donors. Most studies using PTCy have been retrospective making conclusions difficult. ANZHIT-1 was a phase 2 study conducted at 6 Australian allogeneic HSCT centers. The primary end points were disease-free and overall survival at 2 years after HSCT. The reduced-intensity conditioning (RIC) included fludarabine, cyclophosphamide, and 200 cGy total body irradiation, and the myeloablative conditioning (MAC) was IV fludarabine and busulfan. PTCy, MMF and a calcineurin inhibitor (CNI) were used for graft-versus-host disease (GVHD) prophylaxis. CNIs were weaned and ceased by day +120 in eligible patients on day 60. Patients (n = 78) with hematological malignancies were included in the study, with a median follow-up of 732 days (range, 28-1728). HSCT was RIC in 46 patients and MAC in 32 patients. Disease-free survival probability at 2 years was 67.5% (95% [CI], 53.2-85.6) for MAC recipients and 68.3% (95% CI, 56.3-83.01) for RIC recipients. Transplant-related mortality (TRM) on day 100 and year 1 was 4.9% (95% CI, 1.6-15.3) and 17.9% (95% CI, 8.8-36.5), respectively, in the MAC group compared with 3.1% (95% CI, 0.8.1-12) and 11.6% (95% CI, 6-22.4), respectively, in the RIC group. The median time for elective cessation of CNI was day 142.5 days, with no excess chronic GVHD (cGVHD) or mortality. Of the evaluable patients, 71.6% discontinued immunosuppression 12 months after transplant. This prospective haplo-HSCT trial using PTCY demonstrated encouraging survival rates, indicating that early CNI withdrawal is feasible and safe.
Ultrasensitive chimerism enhances measurable residual disease testing after allogeneic hematopoietic cell transplantation
Authors: Kanaan Sami B. , Urselli Francesca , Radich Jerald P. , Nelson J. Lee
Published: July 2023

Increasing mixed chimerism (reemerging recipient cells) after allogeneic hematopoietic cell transplant (allo-HCT) can indicate relapse, the leading factor determining mortality in blood malignancies. Most clinical chimerism tests have limited sensitivity and are primarily designed to monitor engraftment. We developed a panel of quantitative polymerase chain reaction assays using TaqMan chemistry capable of quantifying chimerism in the order of 1 in a million. At such analytic sensitivity, we hypothesized that it could inform on relapse risk. As a proof-of-concept, we applied our panel to a retrospective cohort of patients with acute leukemia who underwent allo-HCT with known outcomes. Recipient cells in bone marrow aspirates (BMAs) remained detectable in 97.8% of tested samples. Absolute recipient chimerism proportions and rates at which these proportions increased in BMAs in the first 540 days after allo-HCT were associated with relapse. Detectable measurable residual disease (MRD) via flow cytometry in BMAs after allo-HCT showed limited correlation with relapse. This correlation noticeably strengthened when combined with increased recipient chimerism in BMAs, demonstrating the ability of our ultrasensitive chimerism assay to augment MRD data. Our technology reveals an underappreciated usefulness of clinical chimerism. Used side by side with MRD assays, it promises to improve identification of patients with the highest risk of disease reoccurrence for a chance of early intervention.
Understanding a mass in the paraspinal region: an anatomical approach
Authors: Creze Maud , Ghaouche Jessica , Missenard Gilles , Lazure Thierry , Cluzel Guillaume , Devilder Matthieu , Briand Sylvain , Soubeyrand Marc , Meyrignac Olivier , Carlier Robert-Yves , Court Charles , Bouthors Charlie
Published: July 2023

The paraspinal region encompasses all tissues around the spine. The regional anatomy is complex and includes the paraspinal muscles, spinal nerves, sympathetic chains, Batson’s venous plexus and a rich arterial network. A wide variety of pathologies can occur in the paraspinal region, originating either from paraspinal soft tissues or the vertebral column. The most common paraspinal benign neoplasms include lipomas, fibroblastic tumours and benign peripheral nerve sheath tumours. Tumour-like masses such as haematomas, extramedullary haematopoiesis or abscesses should be considered in patients with suggestive medical histories. Malignant neoplasms are less frequent than benign processes and include liposarcomas and undifferentiated sarcomas. Secondary and primary spinal tumours may present as midline expansile soft tissue masses invading the adjacent paraspinal region. Knowledge of the anatomy of the paraspinal region is of major importance since it allows understanding of the complex locoregional tumour spread that can occur via many adipose corridors, haematogenous pathways and direct contact. Paraspinal tumours can extend into other anatomical regions, such as the retroperitoneum, pleura, posterior mediastinum, intercostal space or extradural neural axis compartment. Imaging plays a crucial role in formulating a hypothesis regarding the aetiology of the mass and tumour staging, which informs preoperative planning. Understanding the complex relationship between the different elements and the imaging features of common paraspinal masses is fundamental to achieving a correct diagnosis and adequate patient management. This review gives an overview of the anatomy of the paraspinal region and describes imaging features of the main tumours and tumour-like lesions that occur in the region.
PINNED: identifying characteristics of druggable human proteins using an interpretable neural network
Authors: Cunningham Michael , Pins Danielle , Dezső Zoltán , Torrent Maricel , Vasanthakumar Aparna , Pandey Abhishek
Published: July 2023

The identification of human proteins that are amenable to pharmacologic modulation without significant off-target effects remains an important unsolved challenge. Computational methods have been devised to identify features which distinguish between “druggable” and “undruggable” proteins, finding that protein sequence, tissue and cellular localization, biological role, and position in the protein–protein interaction network are all important discriminant factors. However, many prior efforts to automate the assessment of protein druggability suffer from low performance or poor interpretability. We developed a neural network-based machine learning model capable of generating druggability sub-scores based on each of four distinct categories, combining them to form an overall druggability score. The model achieves an excellent performance in separating drugged and undrugged proteins in the human proteome, with an area under the receiver operating characteristic (AUC) of 0.95. Our use of multiple sub-scores allows the assessment of potential protein targets of interest based on distinct contributors to druggability, leading to a more interpretable and holistic model to identify novel targets.
May microbial ecological baseline exist in continental groundwater?
Authors: Zhong Sining , Zhou Shungui , Liu Shufeng , Wang Jiawen , Dang Chenyuan , Chen Qian , Hu Jinyun , Yang Shanqing , Deng Chunfang , Li Wenpeng , Liu Juan , Borthwick Alistair G. L. , Ni Jinren
Published: July 2023

Background

Microbes constitute almost the entire biological community in subsurface groundwater and play an important role in ecological evolution and global biogeochemical cycles. Ecological baseline as a fundamental reference with less human interference has been investigated in surface ecosystems such as soils, rivers, and ocean, but the existence of groundwater microbial ecological baseline (GMEB) is still an open question so far.

Results

Based on high-throughput sequencing information derived from national monitoring of 733 newly constructed wells, we find that bacterial communities in pristine groundwater exhibit a significant lateral diversity gradient and gradually approach the topsoil microbial latitudinal diversity gradient with decreasing burial depth of phreatic water. Among 74 phyla dominated by Proteobacteria in groundwater, Patescibacteria act as keystone taxa that harmonize microbes in shallower aquifers and accelerate decline in bacterial diversity with increasing well-depth. Decreasing habitat niche breadth with increasing well-depth suggests a general change in the relationship among key microbes from closer cooperation in shallow to stronger competition in deep groundwater. Unlike surface-water microbes, microbial communities in pristine groundwater are predominantly shaped by deterministic processes, potentially associated with nutrient sequestration under dark and anoxic environments in aquifers.

Conclusions

By unveiling the biogeographic patterns and mechanisms controlling the community assembly of microbes in pristine groundwater throughout China, we firstly confirm the existence of GMEB in shallower aquifers and propose Groundwater Microbial Community Index (GMCI) to evaluate anthropogenic impact, which highlights the importance of GMEB in groundwater water security and health diagnosis.
Neodymium magnets migrated into an internal supravesical hernia: a rare case of foreign body ingestion in children
Authors: Ikegami Michiaki , Miyaki Yuichiro , Hamano Takashi , Taira Yurina , Takahashi Toshiaki
Published: July 2023

Background

Foreign body (FB) ingestion is a common event in children. The management of the ingested FB depends on the location, type, number, size of the FBs, patient age, and symptoms. Although most FBs pass spontaneously through the gastrointestinal tract without causing serious injuries, the ingestion of multiple high-powered magnetic pieces, especially neodymium magnets (NMs) increases the risks of morbidity and mortality. Supravesical hernia (SH) is rarely occurs in children, and few studies have reported SH in pediatric patients. We report an extremely rare case of ingested NMs that migrated into an internal SH in a pediatric patient.

Case presentation

A 3-year-old boy who had accidentally swallowed two NMs 3 days ago presented with vomiting and lower abdominal pain. X-ray imaging and computed tomography (CT) suspected the presence of a 1.0-cm radiopaque FB located in the terminal ileum dorsal side of the bladder. Although his abdominal pain was gradually getting better after oral feeding, repeat abdominal X-ray imaging showed that the FB was in a stagnant in position. Therefore, surgical intervention was planned to remove the FB 1 week after his admission. Under general anesthesia, laparoscopic and fluoroscopic examinations were performed and the cecum was found adhered to the retroperitoneum between the right medial umbilical fold and the right wall of the urinary bladder. The FB was presumed to be located at the tip of the incarcerated cecum in the retroperitoneal space. Peritoneum incision was started near the medial inguinal fossa, and the Retzius space was opened in a manner similar to the transabdominal pre-peritoneal approach for inguinal hernia repair. Consequently, the patient was diagnosed with internal SH with FB migration. The incarcerated cecum was pulled out, which revealed intestinal wall perforation. The FB remained in the retroperitoneal space in the pelvic cavity. The FB was easily removed using intestinal forceps and identified as combined two NMs. The postoperative course was good, and the patient was discharged on postoperative day 5.

Conclusions

We experienced an extremely rare case of a pediatric patient who swallowed multiple NMs that migrated into an internal SH, and the laparoscopic minimally invasive removal was successful.
Supporting validity: steps to contextualise applications for technology and assessment, for learning
Authors: Kemp Sandra , Goddard Viktoria C. T. , Boursicot Katharine , Fuller Richard , Nadarajah Vishna Devi
Published: July 2023

In the paper, the authors offer perspectives on the uses of technology and assessment, that support learning. The perspectives are viewed through validity (from the field of assessment) as a framework and they discuss four aspects of an interconnected technology, learning and assessment space that represent theory informed, authentic practice. The four are: 1) integrated coherence for learning, assessment and technology; 2) responsibilities for equity, diversity, inclusion and wellbeing; 3) sustainability; and 4) balancing resources in global contexts. The authors propose steps and considerations for medical and health professions educators who need to contextualise applications for technology, learning and assessment, for positive impact for learners, faculty, institutions and patient care.
The genome sequence of the hawkweed Cheilosia, Cheilosia urbana (Meigen, 1822)
Authors: Falk Steven , Gorše Iva ,   University of Oxford and Wytham Woods Genome Acquisition Lab,   Darwin Tree of Life Barcoding collective,   Wellcome Sanger Institute Tree of Life programme,   Wellcome Sanger Institute Scientific Operations: DNA Pipelines collective,   Tree of Life Core Informatics collective,   Darwin Tree of Life Consortium
Published: July 2023

We present a genome assembly from an individual female Cheilosia urbana (the hawkweed Cheilosia; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 546.9 megabases in span. Most of the assembly is scaffolded into 5 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 17.08 kilobases in length.
Modelling and mapping of soil erosion risk based on GIS and PAP/RAC guidelines in the watershed of Tassaoute (Central High-Atlas, Morocco)
Authors: Ziadi Khalid , Barakat Ahmed , El Aloui Abdenbi , Ouayah Mustapha , Namous Mustapha
Published: July 2023

Morocco watersheds, which provided many ecosystem services necessary for the socio-economic life of rural communities, are experiencing significant change and environmental problems. Therefore, examining potential soil erosion considered a major problem in the Moroccan highlands is very important to prioritize high erosion severity areas. Keeping in view of the above aspects, the present study aimed to evaluate and map areas at risk of water erosion in the upstream Tassaoute watershed (central High Atlas, Morocco), using the Priority Action Program/Regional Activity Center (PAP/RAC) method associated with Geographic Information Systems (GIS) and remote sensing. The PAP/RAC approach consisted of integrating the natural factors that influence water erosion, namely slope, lithology, vegetation cover and land use. This method provided an accurate cartographic product that reflects the reality of the state of soil degradation and the qualitative assessment of erosion. The generated erosion risk map of the study area showed that the phenomenon of erosion threatens this basin, especially in the middle and downstream, such that 40% of the basin surface has significant erosion and the high and very high degree of erosion represented 27% of the total surface of the study area. These results therefore demonstrated the PAP/CAR model reliability in assessing and mapping of water erosion risks in the upstream Tassaoute basin.
Minimizing Compositions of Differences-of-Convex Functions with Smooth Mappings
Authors: Le Thi Hoai An , Huynh Van Ngai , Dinh Tao Pham
Published: July 2023

We address the so-called DC (difference-of-convex functions) composite minimization problems (or DC composite programs) whose objective function is a composition of a DC function with a continuously differentiable mapping. We first develop an algorithm named DC composite algorithm (DCCA in short) for unconstrained DC composite programs and further extend to DC composite programs with constraints of inclusion associated with a smooth mapping and a closed convex set. The convergence analysis of the proposed algorithms is investigated. Applications of DCCA for two different problems, computation of the numerical radius of a square matrix and minimization of composite energies, are presented.
Tropical climate change and its impact on horticultural plants in Enrekang District, South Sulawesi, Indonesia
Authors: YASSI AMIR , DEMMALLINO EYMAL B. , SULTANI HAIRUL RACHMAN
Published: July 2023

Abstract.Yassi A, Demmallino EB, Sultani HR. 2023. Tropical climate change and its impact on horticultural plants in Enrekang District, South Sulawesi, Indonesia. Biodiversitas 24: 3073-3079. Global, regional, and local changes in climate are phenomenologically different and have distinct impacts on the horticultural and agricultural sectors. Therefore, this research aims to examine tropical climate change in South Sulawesi, Indonesia and analyze its impact on horticultural crops. Apost-positivistic interpretation framework of the case study tradition was used, while the location was determined by purposive sampling followed by comprehensive data collection. Data were analyzed using integrated techniques, and validity tests were carried outto assess thecredibility and reliability. The results indicated a local climate change marked by a differencein the pattern of distribution and intensity of extreme rainfall between 2015 and 2018. This was due toan increase in air temperature, which on average reached ±0.03731°C from the lowest of 22.50°C in 2011 to the highest of 23.12°C in 2020. In other words, it can be stated that the air temperature had increased by ±0.63°C in the last decade. The impact on various types of horticultural crops was marked by a decrease in the production of several kinds of Chinese cabbage,carrots, and green beans in 2015 as well asa very drastic reductionin potato crop production in 2018, while cabbage plants experienced a peak in production in 2018. Some types of horticultural crops such as shallots, cabbage and red chili began to stabilize and even increased their production in the aftermath of climate changeduring said period.
Predicting of Komodo dragon's potential prey habitat suitability using MaxEnt in Riung Nature Reserve, Flores, East Nusa Tenggara, Indonesia
Authors: PRAMATANA FADLAN , AINI YUSRATUL , RAMMANG NIXON , MAU YOSEP SERAN , ARSA I G.B. ADWITA , MAHMUD ARIEF
Published: July 2023

Abstract. Pramatana F, Aini Y, Rammang N, Mau YS, Arsa IGBA, Mahmud A. 2023. Predicting of Komodo dragon's potential prey habitat suitability using MaxEnt in Riung Nature Reserve, Flores East Nusa Tenggara. Biodiversitas 24: 3128-3139. The Komodo dragon (Varanus komodoensis Ouwens, 1912) is a big lizard species from the Varanidae family that belongs to the Endangered category (EN) listed on the IUCN red list and Appendix I CITES. This study aimed to reveal the distribution of potential preys Komodo dragons in Rinca Island, Komodo National Park, Manggarai District, East Nusa Tenggara, Indonesia, using Maximum Entropy (MaxEnt), which was collected using rapid assessment methods. The presences of the Komodo dragon's potential prey come from direct and indirect observation or previous studies. We collected 510 points of Komodo dragon prey presence in Riung, Ngada District, East Nusa Tenggara, Indonesia from six species, including cattle, but only used 127 points for analysis based on the correlation. Long-tailed macaque, wild boar, civet, Timor deer, feral horses, and cows were the potential prey for komodo in Riung. Most of the points come from cattle, such as cows. On the other hand, we used environmental habitat to represent prey habitats such as elevation, slope, land surface temperature, moisture index, vegetation index, and distance from specific objects such as distance from agriculture, rivers, road, savanna, and settlement. Komodo dragon's potential prey in Riung was distributed in savanna, mangrove, and lowland forest. The result showed three suitable habitats for the Komodo dragon's potential prey dominated by low and moderate-suitability areas.
Physiological aging in India: The role of the epidemiological transition
Authors: Krenz Astrid , Strulik Holger
Published: July 2023

We construct a cohort-based frailty index from age-related health deficits to investigate physiological aging in India over the period 1990-2019. During this period, the Indian states underwent at different speeds the epidemiological transition and experienced unprecedented economic growth. We show that the rate of physiological aging remained remarkably stable to the changing environment. Age-related health deficits increased by about 3 percent per year of age with little variation across states, ages, cohorts, and over time. We find that, with advancing epidemiological transition, health deficits for given age declined at the individual level (within states and within cohorts). Across cohorts born between 1900 and 1995, we show that, for given age, health deficits are higher for later-born cohorts until birth years around 1940 and remained trendless afterwards. We propose a selection-based theory of aging during the epidemiological transition that explains these facts.
Solute carrier family 35 member A2 (SLC35A2) is a prognostic biomarker and correlated with immune infiltration in stomach adenocarcinoma
Authors: Huang Zigao , Yang Hong , Lao Jingmao , Deng Wei
Published: July 2023

Background

Solute carrier family 35 member A2 (SLC35A2) located on the X chromosome is considered involved in the UDP-galactose transport from cytosol to Golgi apparatus and endoplasmic reticulum. It has been reported that the SLC35A2 expression is associated with carcinogenesis in recent studies, however, its specific roles in cancer progression have not been exhaustively elucidated. Herein, a system analysis was conducted to evaluate the role of SLC35A2 in prognostic, and immunology in stomach adenocarcinoma (STAD).

Methods

The TIMER, GEPIA, UALCAN, Kaplan–Meier Plotter were employed to explore the SLC35A2 expression pattern and prognostic value in STAD. Genomic alterations were searched through the MEXPRESS and cBioPortal platforms. The LinkedOmics, GEPIA and Metascape databases were employed to explore the biological processes. The TIMER and TISIDB websites were utilized to investigate the relationships between SLC35A2 expression and immune cell infiltration. The associations between SLC35A2 expression and tumor mutational burden (TMB), microsatellite instability (MSI) in pan-cancer were explored using the SangerBox database.

Results

Compared to the normal gastric mucosa, SLC35A2 expression was significantly increased in STAD tissues, accompanied by the robust relationships with tumor grade, histological subtypes, TP53 mutation status, TMB and prognosis. SLC35A2 and its co-expression genes played the primarily roles in purine metabolism and purinosome, including the asparagine N-linked glycosylation, protein processing in endoplasmic reticulum, regulation of transcription involved in G1/S transition of mitotic cell cycle, with the potential to participate in the regulation of VEGFA-VEGFR2 signaling pathway. Concurrently, SLC35A2 expression was correlated with macrophages and CD4+T lymphocytes infiltration in STAD.

Conclusions

Our study has proposed that SLC35A2 correlated with immune cell infiltration could serve as a prognostic biomarker in STAD.
An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure for the quantification of topiramate in human serum and plasma
Authors: Salzmann Linda , Spescha Tino , Singh Neeraj , Kobel Anja , Fischer Vanessa , Schierscher Tobias , Bauland Friederike , Geistanger Andrea , Risch Lorenz , Geletneky Christian , Seger Christoph , Taibon Judith
Published: July 2023

Objectives

Topiramate is an antiepileptic drug (AED) used for the monotherapy or adjunctive treatment of epilepsy and for the prophylaxis of migraine. It has several pharmacodynamic properties that contribute to both its clinically useful properties and observed adverse effects. Accurate measurement of its concentration is therefore essential for dose adjustment/optimisation of AED therapy. Our aim was to develop and validate a novel reference measurement procedure (RMP) for the quantification of topiramate in human serum and plasma.

Methods

An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) method in combination with a protein-precipitation-based sample preparation allows for quantification of topiramate in human serum and plasma. To assure traceability to SI units, quantitative nuclear magnetic resonance (qNMR) was applied to characterize the reference material used as primary calibrator for this RMP. Matrix effects were determined by performing a post-column infusion experiment and comparing standard line slopes. Accuracy and precision was evaluated performing an extensive five day precision experiment and measurement uncertainty was evaluated according Guide to the Expression of Uncertainty in Measurement (GUM).

Results

The method enabled topiramate quantification within the range of 1.20–36.0 μg/mL without interference from structurally related compounds and no evidence of a matrix effect. Intermediate precision was ≤3.2 % and repeatability was 1.4–2.5 % across all concentration levels. The relative mean bias was −0.3 to 3.5 %. Expanded measurement uncertainties for target value assignment (n=6) were found to be ≤2.9 % (k=2) independent of the concentration level and the nature of the sample.

Conclusions

In human serum and plasma, the RMP demonstrated high analytical performance for topiramate quantification and fulfilled the requirements on measurement uncertainty. Traceability to SI units was established by qNMR content determination of the topiramate, which was used for direct calibration of the RMP. This RMP is, therefore, fit for purpose for routine assay standardization and clinical sample evaluation.
The GLP-1 agonist, exendin-4, stimulates LH secretion in female sheep
Authors: Simpson Elizabeth M , Clarke Iain J , Scott Christopher J , Stephen Cyril P , Rao Alexandra , Gunn Allan J
Published: July 2023

Our previous studies showed that microinjection into the median eminence of the sheep of glucagon-like peptide- 1 (GLP-1) or its receptor agonist exendin-4 stimulates luteinising hormone (LH) secretion, but it is unknown whether the same effect may be obtained by systemic administration of the same. The present study measured the response in terms of plasma LH concentrations to intravenous (iv) infusion of exendin-4. A preliminary study showed that infusion of 2 mg exendin-4 into ewes produced a greater LH response in the follicular phase of the oestrous cycle than the luteal phase. Accordingly, the main study monitored plasma LH levels in response to either 0.5 mg or 2 mg exendin-4 or vehicle (normal saline) delivered by jugular infusion for 1 h in the follicular phase of the oestrous cycle. Blood samples were collected at 10 min intervals before, during and after infusion. Both doses of exendin-4 increased mean plasma LH concentrations and increased LH peripheral pulse amplitude. There was no effect on inter-pulse interval or timing of the preovulatory LH surge. These doses of exendin-4 did not alter plasma insulin or glucose concentrations. Quantitative PCR of the gastrointestinal tract samples from a population of ewes confirmed the expression of the preproglucagon gene (GCG). Expression increased aborally and was greatest in the rectum. It is concluded that endogenous GLP-1, most likely derived from the hindgut, may act systemically to stimulate LH secretion. The present data suggest that this effect may be obtained with levels of agonist that are lower than those functioning as an incretin.
Bullying e interações familiares: comparações entre meninos e meninas por meio da triangulação metodológica
Authors: Oliveira Wanderlei Abadio de , Silva Jorge Luiz da , Caravita Simona Carla Silvia , Silva Marta Angélica Iossi , Santos Manoel Antônio dos
Published: July 2023

O bullying afeta o desenvolvimento dos adolescentes. Este estudo objetivou comparar experiências de bullying e variáveis das interações familiares entre meninos e meninas. A amostra foi composta por 2.354 estudantes (50,7% meninas; M = 14,5 anos, DP = 2,0) que responderam duas escalas sobre bullying e interações familiares. 55 adolescentes participaram de entrevistas semiestruturadas. Os dados quantitativos foram analisados por meio do teste t de Student e o índice d de Cohen. A análise das entrevistas foi desenvolvida no software Atlas.TI. Identificou-se que os meninos eram mais agressores e vítimas-agressoras. Os resultados mistos indicaram poucas diferenças entre meninos e meninas no que se refere às variáveis analisadas. A comunicação positiva foi fator protetivo para as meninas, ao passo que o clima conjugal negativo e a punição física foram fatores que aumentam a vulnerabilidade para bullying ou vitimização para ambos os sexos. São discutidas implicações práticas para o enfrentamento do bullying.
COLOMBIA: PARADOJAS DE UN PAIS MARITIMO CON MENTALIDAD MEDITERRANEA
Authors: Bazurto Enrique Roman
Published: July 2023

Así como del mar surgió la vida, también apareció el hombre en una lenta evolución a lo largo de millones de años. Con el pausado devenir de los siglos, los líquidos caminos del océano fueron conduciendo a las Américas sus primeros pobladores oriundos del Asia y Polinesia, quizá también de Africa; o tal vez fue lo contrario, según las últimas teorías.
Historia Caribe Vol. 12 Núm. 30 (2017): Enero - Junio
Authors: Editorial Comité
Published: July 2023

Historia Caribe Vol. 12 Núm. 30 (2017): Enero - Junio
Historia Caribe Vol. 13 Núm. 32 (2018): Enero-Junio
Authors: Editorial Comité
Published: July 2023

Vol. 13 Núm. 32 (2018): Enero-Junio
Historia Caribe Vol. 13 Núm. 33 (2018): Julio-Diciembre
Authors: Editorial Comité
Published: July 2023

Vol. 13 Núm. 33 (2018): Julio-Diciembre
Historia Caribe Vol. 15 Núm. 36 (2020): Enero-Junio
Authors: Editorial Comité
Published: July 2023

Vol. 15 Núm. 36 (2020): Enero-Junio
Historia Caribe Vol. 15 Núm. 37 (2020): Julio-Diciembre
Authors: Editorial Comité
Published: July 2023

Historia Caribe Vol. 15 Núm. 37 (2020): Julio-Diciembre
Historia Caribe Vol. 16 Núm. 39 (2021): Julio - Diciembre
Authors: Editorial Comité
Published: July 2023

Historia Caribe Vol. 16 Núm. 39 (2021): Julio - Diciembre
En medio de las turbulentas pasiones: el uxoricidio en el Nuevo Reino de Granada (1779-1810)
Authors: López Solórzano Melvys Ornella
Published: July 2023

El presente artículo estudia el delito de uxoricidio a partir de tres casos ocurridos en el Nuevo Reino de Granada entre 1779-1810. Los móviles para que mujeres de diferentes calidades fueran asesinadas por sus cónyuges iban desde las ofensas al honor de los hombres, hasta el “incumplimiento” de las obligaciones conyugales. El estudio centra la atención en las calidades de las personas involucradas en el acto delictuoso, con un doble propósito. Por una parte, examinar las justificaciones que se le atribuyeron, de parte de los procuradores de pobres, al delito imputado y, por la otra, ver la correlación entre la calidad del condenado y la pena que se le impuso. Argumentos como el honor de los de abajo, la fe conyugal, la ceguera emocional y la irracionalidad propia de la condición de indio fueron esgrimidos por los defensores para obtener penas bastantes benévolas para sus defendidos, si se toma en cuenta la magnitud del delito.
Natalia Silva Prada, Pasquines, cartas y enemigos. Cultura del lenguaje infamante en la Nueva Granada y otros reinos americanos, siglos XVI y XVII. Bogotá: Editorial Universidad del Rosario (Textos de Ciencias Humanas), 2021.
Authors: Alzate Echeverri Adriana María
Published: July 2023

Este libro de la historiadora Natalia Silva Prada es el resultado de muchos años de trabajo sobre la problemática del lenguaje infamante en el mundo hispanoamericano de los siglos XVI y XVII. El libro concentra y amplía sus investigaciones al respecto, publicadas en formatos más reducidos como artículos y capítulos de libro desde hace 20 años. Es una obra madura, que interroga algunas ideas que se han ido volviendo lugar común sobre la historia de la cultura política en el mundo hispanoamericano colonial temprano.
José Trinidad Polo Acuña y Rafael Enrique Acevedo Puello. Circulación, negocios y libros en la República de Colombia Siglo XIX y Primera mitad del siglo XX. Medellín: La Carreta Editores, 2019.
Authors: García Charris Eva Sandrin
Published: July 2023

Los editores, José Trinidad Polo Acuña, doctor en Historia por la Universidad Central De Venezuela, y Rafael Enrique Acevedo Puello, doctor en Historia por la Universidad de los Andes (Colombia), quienes además son autores, nos presentan en esta oportunidad un libro fundamental para comprender procesos de carácter históricos que tiene como eje articulador la noción de circulación. Por una parte, aplicada a libros, ideas e imágenes que fueron elementos claves para comprender el desarrollo de la sociedad. Además, nos permite identificar su vinculación con la aparición de importantes figuras como los autores, lectores y el papel que representaron las bibliotecas en el caso particular de pueblos de la región Caribe. Por la otra, conectada esta noción directamente a procesos de orden económico que se desarrollaron en diferentes espacios del Caribe de Colombia en los que emergieron comerciantes, negociantes y empresarios. Estas variables poco han sido abordadas en la historiografía colombiana, por lo cual, la obra constituye una invitación y punto de arranque para empezar a estudiar el tema.
Mujeres y cooperación salesiana en ciudades y pueblos argentinos (fines del siglo XIX a 1930)
Authors: Bracamonte Lucía
Published: July 2023

Este artículo tiene por objetivo analizar los roles de cooperadoras salesianas laicas de distintas ciudades y pueblos de la Argentina entre fines del siglo XIX y 1930. A través de cartas, se estudia a aquellas que no integraban comisiones de la Pía Unión de Cooperadores Salesianos y que, por ello, han dejado menos huellas documentales y tenido menor visibilidad historiográfica que las que sí lo hacían. Algunas de estas cooperadoras no se limitaron a cumplir sus deberes como integrantes de esa tercera orden, sino que actuaron para obtener otros beneficios para sus personas, sus núcleos parentales o emprendimientos religiosos de sus comunidades.
De la participación a la acción política. Las secciones femeninas de los partidos políticos en Cuba, 1925-1959
Authors: Ramírez Chicharro Manuel
Published: July 2023

Durante la República, los partidos políticos cubanos crearon secciones dentro de sus estructuras, como la laboral y la estudiantil. Este artículo estudia cómo las secciones femeninas, creadas en el contexto de las luchas sufragistas, fueron utilizadas por los partidos políticos para extender su doctrina entre las potenciales votantes. Poco después, estas asumieron competencias más allá del apoyo propagandístico, desarrollando campañas educativas o integrando la defensa civil de Cuba durante la II Guerra Mundial. Después, en los años cincuenta, algunas llegaron a constituir importantes vías de inserción de las mujeres en la insurrección contra la dictadura de Fulgencio Batista.
Arribo del cinematógrafo al exótico Caribe. Elementos para un acercamiento comparado a la industria del cine de la región
Authors: López Lizarazo Carlos
Published: July 2023

Este artículo mira críticamente el arribo del cinematógrafo Lumière al Caribe, mediante un análisis comparado en algunos de sus países, con el objetivo de analizar su impacto en las prácticas culturales de una coyuntura bisagra entre los siglos XIX y XX. Prácticas afectadas con el nuevo invento en la constitución identitaria regional mediante el consumo de películas y luego por su producción en clave exótica. Clave que orientó las prácticas de comunicación sobre la región y la presentaron entre la clásica convención exótica de barbarie, atraso o salvajes y el deseo de sus élites de presentarse como “modernos”.
S. S. Zimnitsky — the creator of the functional direction in internal medicine. The outset — research in gastroenterology
Authors: Оslopov V. N. , Khazova E. V. , Khasanov N. R. , Oslopova Yu. V. , Mishanina Yu. S. , Oslopova D. V.
Published: July 2023

Semen Semenovich Zimnitsky is an outstanding Russian internist of the early XX century. He created a functional direction and made a significant contribution to internal medicine, infectiology, immunology and microbiology, but his studies of gastroenterology are fundamental. The Zimnitsky’s works on hepatology, pancreatic diseases, and balneology are interesting. S. S. Zimnitsky discovered 5 types of gastric secretion, which turned out to be reversible, and proposed a repeated breakfast. The pinnacle of his work is Zimnitsky’s test, which makes it possible to determine the renal function without resorting to any invasive procedures and in no way burdening the patient. The onset of test creation was laid by S. S. Zimnitsky in his doctoral dissertation devoted to gastroenterology.
Social attractiveness of Russian higher medical education for international students
Authors: Chumakov V. I. , Farmanyan R. R.
Published: July 2023

The article is devoted to the study of social attractiveness of Russian higher medical education for foreign students. The importance of promotion of educational services of Russian universities is highlighted. Criteria for choosing a university by foreign students were assessed. The importance of modern and effective communication channels of the university and foreign applicants, modern infrastructure and learning technologies was determined. The work contains recommendations for improving the attractiveness of medical universities for foreigners.
The aims of humanities in the training of medical students on the example of history subjects
Authors: Chumakov V. I.
Published: July 2023

The article is devoted to the study of the place of history subjects in the formation of general cultural competencies of medical students. A theoretical analysis of the topic in domestic and foreign history and pedagogical science has been carried out. The necessity of history subjects in the system of higher professional medical education is substantiated. Based on many years of experience, pedagogical technologies developed by the authors are presented that most fully form general cultural and professional competencies within historical subjects. The data of pedagogical research on the topic are given, the opinions of students on the content of history subjects, on the process of forming knowledge, skills and abilities of medical students are explicated. The practical significance of the study for improving the pedagogical process in a medical university is reflected.
Patterns of localized deformation at pre-fracture stage in carbon steel – stainless steel bimetal
Authors: Barannikova S. A. , Li Yu. V.
Published: July 2023

The work is devoted to the study of strain localization at macroscale level during parabolic mechanical hardening and pre-fracture under quasi-static loading of a carbon steel – stainless steel bimetal. The problem of estimating the scale of the phenomena that determine plasticity is decisive in the development of any theories of plastic deformation, in particular, dislocation theories. The main difficulty in constructing such theories is the reconciling the dislocation scales, characteristic for most deformation and mechanical hardening mechanisms, with macroscopic parameters of deformation processes. In the framework of the autowave model of localized plastic deformation, this problem can be reduced to the possibility of obtaining parameters from the results of macroscale observations of localized plastic flow development. During the experiments, it was confirmed that in a bimetal at any forming stage, a specific pattern of localization centers distribution is spontaneously generated - a pattern of localized plastic flow. The shape of such patterns is determined by the law of mechanical hardening acting in the material. It is shown that the observed localization patterns can be used as an informative feature in predicting the plasticity margin. In the process of uniaxial tension at the stage of parabolic mechanical hardening of the bimetal, the deformation mode is realized with the formation of several potential fracture centers. It was established that at the pre-fracture stage, during the time evolution of the wave pattern of deformation localization, the zone of active plastic deformation narrows, but the number of centers in it either remains the same with a decrease in the distance between them, or even increases. The result of this process is the formation of a macroscopic neck, and then fracture. At the pre-fracture stage, the collapse point indicates the place of future fracture and signals the need to stop the deformation process in order to avoid the fracture of the bimetallic material. Thus, the well-known manifestation of deformation macroscopic localization – formation of a neck – is preceded by complex phenomena of mutually coordinated motion of localized plasticity centers at the pre-fracture stage.
Lead and zinc selective extraction from EAF dust while heating in resistance furnace with flowing argon
Authors: Podusovskaya N. V. , Komolova O. A. , Grigorovich K. V. , Pavlov A. V. , Aksenova V. V. , Rumyantsev B. A. , Zheleznyi M. V.
Published: July 2023

The elemental and phase compositions of electric arc furnace (EAF) dust from PJSC Severstal were studied. We carried out the thermodynamic modeling of zinc and lead selective extraction process and determined its possible mechanisms. EAF dust was heated in the temperature range of 20 – 1300 °C in vacuum resistance furnace and the Tamman furnace with flowing argon. Experiments in the vacuum resistance furnace with linear heating showed that lead and zinc removal from the sample occurs in the temperature range of 800 – 1200 °C, with higher lead removal rate. Intensive lead removal was observed at temperature above 1000 °C, while intensive zinc removal occurs at temperature above 1200 °C. Clarifying isothermal experiments performed in the Tamman furnace showed that lead complete transition to the gas phase was achieved at a temperature of 1100 °C (holding time – 12 min) and at a temperature of 1200 °C (holding time – 6 min or more). At the same time, zinc removal was observed in the amount of 14.4 % ratio and 32.2 % ratio, respectively, which allows us to conclude that it is possible to consistently obtain two products: lead and zinc mixture and zinc not contaminated with lead. When comparing experimental and thermodynamic modeling data, the reactions that are most likely to occur during the carbon reduction of lead- and zinc-containing phases were determined.
Parametric model of a three-roll unit of radial-shear rolling mini-mill
Authors: Troitskii D. V. , Gamin Yu. V. , Galkin S. P. , Budnikov A. S.
Published: July 2023

The article discusses the main structural features of radial-shear rolling mini-mills and their most common sizes. A generalized algorithm for designing such mills using modern CAD systems is described. The main approaches to the methodology of software adaptive design of models in engineering are listed with their features and differences. In particular, the methodology of horizontal modeling, explicit modeling methodology, and resilient modeling strategy are considered. The article describes the method of virtual squeezes and presents the main geometric scheme of the spatial position of the rollers of the longitudinal profile. The data obtained as a result of the calculations were encoded and summarized in tables. The formulas presented were used in the parametric design of the roller unit of the three-roller mill 30-70 using Autodesk Inventor software. The obtained parametric model, using classical formulas of the virtual squeezes method, allows for automatic reconstruction of the deformation zone for new initial parameters. The developed model is applicable for three-roller mills with working roll angles δ = 5 – 15° and feed angles β = 18 – 22°. The article presents sketches and diagrams of the constructed model for different rolling angles – 5, 10, and 15°. As the rolling angle increases, a noticeable increase in the conicity of the roller is observed. The vector of future research on improving the obtained software model was indicated. Further research on improving the parametric model will include expanding the set of existing parameters to include the frame and full set of roller connections – neck, cover, pressing device, etc.