bluuebunny/crossref_metadata_2025 huggingface dataset:
Correction: Mbuyisa et al. Impact of Foliar-Applied Plant Extracts on Growth, Physiological and Yield Attributes of the Potato (Solanum tuberosum L.). Agronomy 2024, 14, 38
Authors: Mbuyisa Siphokuhle , Bertling Isa , Ngcobo Bonga Lewis
Published: April 2024

In the original publication [...]
Epidemiology of Digital Dermatitis in Western Canadian Feedlot Cattle
Authors: Erickson Sarah , Booker Calvin , Song Jiming , Janzen Eugene , Jelinski Murray , Schwartzkopf-Genswein Karen
Published: April 2024

Digital dermatitis (DD) is an emerging disease in feedlot cattle. Our objective was to identify animal- and feedlot-level risk factors for DD by analyzing individual animal health records (n = 1,209,883) and feedlot-level records from western Canadian feedlots (n = 28) between 2014 and 2018, inclusive. The risk of a DD diagnosis was higher (incidence rate ratio (IRR) = 2.08, 95% CI 1.52 to 2.86) in cattle sourced from confined background operations (CB) versus cattle sourced from auction markets (AM). Conversely, ranch direct (RD) cattle were (IRR = 0.02, 95% CI 0.04 to 0.30) lower risk than AM cattle of being diagnosed with DD. The risk of being diagnosed with DD was higher in females than in males. The magnitude of the risk in females over males was influenced by annual DD incidence in low morbidity years (2014, 2017, and 2018) (IRR = 2.02, 95% CI 1.27 to 3.19), medium morbidity years (2016) (IRR = 2.95, 95% CI 1.64 to 5.33), and high morbidity years (2015) (IRR = 5.41, 95% CI 3.27 to 8.95). At the feedlot-level, the risk of a diagnosis of DD was lower in small capacity (SCF) versus large capacity feedlots (LCF) (IRR = 0.24, 95% CI 0.05 to 0.76). Future research should focus on identifying factors that may propagate disease transmission between cattle of different sexes and from different acquisition sources.
DNA Content in Embryonic Extracellular Vesicles Is Independent of the Apoptotic Rate in Bovine Embryos Produced In Vitro
Authors: Caamaño Diego , Cabezas Joel , Aguilera Constanza , Martinez Ioanna , Wong Yat Sen , Sagredo Daniela Sanhueza , Ibañez Belén , Rodriguez Sebastián , Castro Fidel Ovidio , Rodriguez-Alvarez Lleretny
Published: April 2024

Pre-implantation embryos release extracellular vesicles containing different molecules, including DNA. The presence of embryonic DNA in E-EVs released into the culture medium during in vitro embryo production could be useful for genetic diagnosis. However, the vesicles containing DNA might be derived from embryos suffering from apoptosis, i.e., embryos of bad quality. This work intended to confirm that embryos release DNA that is useful for genotyping by evaluating the effect of embryonic apoptosis on DNA content in E-EVs. Bovine embryos were produced by parthenogenesis and in vitro fertilization (IVF). On Day 5, morulae were transferred to individual cultures in an EV-depleted SOF medium. On Day 7, embryos were used to evaluate cellular apoptosis, and each culture medium was collected to evaluate E-EV concentration, characterization, and DNA quantification. While no effect of the origin of the embryo on the apoptotic rate was found, arrested morulae had a higher apoptotic rate. E-EVs containing DNA were identified in all samples, and the concentration of those vesicles was not affected by the origin or quality of the embryos. However, the concentration of DNA was higher in EVs released by the arrested parthenogenetic embryos. There was a correlation between the concentration of E-EVs, the concentration of DNA-positive E-EVs, and the concentration of DNA. There was no negative effect of apoptotic rate on DNA-positive E-EVs and DNA concentration; however, embryos of the best quality with a low apoptotic rate still released EVs containing DNA. This study confirms that the presence of DNA in E-EVs is independent of embryo quality. Therefore, E-EVs could be used in liquid biopsy for noninvasive genetic diagnosis.
Surgical Correction of a Sinus Venosus Atrial Septal Defect with Partial Anomalous Pulmonary Venous Connections Using Cardiac Computed Tomography Imaging and a 3D-Printed Model
Authors: Kim Kyung-Min , Moon Chang-Hwan , Lee Won-Jong , Kim Woo-Jin , Kim Mihyung , Jeong Jaemin , Lee Hae-Beom , Jeong Seong-Mok , Choi Ho-Jung , Hwang Tae Sung , Lee Hee Chun , Yu Jae Hyeon , Nam Aryung , Kim Dae-Hyun
Published: April 2024

Sinus venosus atrial septal defects (SVASDs), concurrent with partial anomalous pulmonary venous connections (PAPVCs), are a rare congenital heart disease in dogs. Surgical correction is essential when clinical signs or significant hemodynamic changes are present. We aimed to report on the successful surgical correction of an SVASD with PAPVCs, using a computed tomography (CT)-based customized 3D cardiac model. A 10-month-old male poodle was referred for corrective surgery for an ASD. Echocardiography confirmed a hemodynamically significant left-to-right shunting flow through an interatrial septal defect and severe right-sided heart volume overload. For a comprehensive diagnosis, a CT scan was performed, which confirmed an SVASD with PAPVCs. A customized 3D cardiac model was used for preoperative decision-making and surgical rehearsal. The defect was repaired using an autologous pericardial patch under a cardiopulmonary bypass (CPB). Temporary pacing was applied for sinus bradycardia and third-degree atrioventricular block. The patient recovered from the anesthesia without further complications. The pacemaker was removed during hospitalization and the patient was discharged without complications 2 weeks post-surgery. At the three-month follow-up, there was no shunting flow in the interatrial septum and the right-sided volume overload had been resolved. The cardiac medications were discontinued, and there were no complications. This report indicates the validity of surgical correction under CPB for an SVASD with PAPVCs, and the advantages of utilizing a CT-based 3D cardiac model for preoperative planning to increase the surgical success rate.
Genetic Variation and Composition of Two Commercial Estonian Dairy Cattle Breeds Assessed by SNP Data
Authors: Värv Sirje , Põlluäär Tõnu , Sild Erkki , Viinalass Haldja , Kaart Tanel
Published: April 2024

The aims of this study were to assess the genomic relatedness of Estonian and selected European dairy cattle breeds and to examine the within-breed diversity of two Estonian dairy breeds using genome-wide SNP data. This study was based on a genotyped heifer population of the Estonian Red (ER) and Estonian Holstein (EH) breeds, including about 10% of all female cattle born in 2017–2020 (sample sizes n = 215 and n = 2265, respectively). The within-breed variation study focused on the level of inbreeding using the ROH-based inbreeding coefficient. The genomic relatedness analyses were carried out among two Estonian and nine European breeds from the WIDDE database. Admixture analysis revealed the heterogeneity of ER cattle with a mixed pattern showing several ancestral populations containing a relatively low proportion (1.5–37.0%) of each of the reference populations used. There was a higher FROH in EH (FROH = 0.115) than in ER (FROH = 0.044). Compared to ER, the long ROHs of EH indicated more closely related parents. The paternal origin of the genetic material used in breeding had a low effect on the inbreeding level. However, among EH, the highest genomic inbreeding was estimated in daughters of USA-born sires.
Risk Awareness and Attitude of German Farmers towards Biosecurity Measures
Authors: Herrmann Anna , Schulz Katja , Wischnewski Natalie , Brüssau Jule , Zeiler Eva , Sauter-Louis Carola
Published: April 2024

The implementation of management activities depends on both the attitude of the people performing the work and their understanding of why the work should be performed. In the context of animal husbandry, the implementation of such practices is crucial for the functionality of biosecurity. Therefore, it is important to know how farmers perceive biosecurity as a whole. An anonymous online survey was conducted among German farmers. In addition to general data about their farm, information about their existing concept of biosecurity, as well as about the assessment of possible introduction routes for animal diseases into the farm with regard to their likelihood, was gathered. Furthermore, information on measures to protect their farm against disease introduction were retrieved. Analysis showed that in general, farmers were aware of the importance of biosecurity and consequently had concepts of biosecurity on their farms. However, awareness about dangerous introduction routes for animal diseases into a farm was associated with a lack of knowledge of how to improve the measures in these areas. The role of the veterinarian in the context of biosecurity was highlighted and further problematic areas were indicated. Overall, the high level of commitment from farmers indicated a good implementation of daily practices.
Surveillance and Genetic Analysis of Low-Pathogenicity Avian Influenza Viruses Isolated from Feces of Wild Birds in Mongolia, 2021 to 2023
Authors: Kang Yong-Myung , Tseren Ochir Erdene-Ochir , Heo Gyeong-Beom , An Se-Hee , Jeong Hwanseok , Dondog Urankhaich , Myagmarsuren Temuulen , Lee Youn-Jeong , Lee Kwang-Nyeong
Published: April 2024

The introduction of novel highly pathogenic (HPAI) viruses into Korea has been attributed to recombination events occurring at breeding sites in the Northern Hemisphere. This has increased interest in monitoring and genetically analyzing avian influenza viruses (AIVs) in northern regions, such as Mongolia, which share migratory bird flyways with Korea. AIVs in Mongolia were monitored by analyzing 10,149 fecal samples freshly collected from wild birds from April to October in 2021 to 2023. The prevalence of AIVs in wild birds was 1.01%, with a total of 77 AIVs isolated during these 3 years. These 77 AIVs included hemagglutinin (HA) subtypes H1, H2, H3, H4, H6, H10 and H13 and neuraminidase (NA) subtypes N1, N2, N3, N6, N7 and N8. The most frequently detected subtype combinations were H3N8 (39.0%) and H4N6 (19.5%), although HPAI viruses were not detected. Genetic analysis indicated that theses AIVs isolated from Mongolian samples were closely related to AIVs in wild birds in Korea, including those of Eurasian lineage. These findings indicate the necessity of continuous AIV surveillance and monitoring, as HPAI viruses introduced into Korea may derive from strains in Mongolia.
The First Report of Pennella (Crustacea: Copepoda) Infesting Stenella coeruleoalba Stranded in Malta: Morphological and Genetic Analyses
Authors: Vella Adriana , Vella Noel
Published: April 2024

Here, we document the stranding of a striped dolphin Stenella coeruleoalba (Meyen, 1833) (Mammalia: Delphinidae), which was found dead in Maltese waters in July 2020. The stranded dolphin exhibited a severe infestation of the mesoparasitic copepod, Pennella balaenoptera Koren and Danielssen, 1877 (Copepoda: Pennelidae). Parasites of this genus represent the largest known mesoparasites to infest cetaceans. Under normal circumstances, cetaceans may have a few P. balaenoptera individuals attached to them, but cetaceans with compromised health are more prone to heavy infestations. The identification of the parasite was accomplished through morphological and genetic analyses. This incident highlights the significance of monitoring mesoparasitic infestations, offering valuable insights into the health of cetacean populations and emphasizing the potential implications for conservation efforts in the region.
The Levels of Cortisol and Selected Biochemical Parameters in Red Deer Harvested during Stalking Hunts
Authors: Dziki-Michalska Katarzyna , Tajchman Katarzyna , Kowalik Sylwester , Wójcik Maciej
Published: April 2024

As a reactive species, the red deer is sensitive to both negative exogenous and endogenous stimuli. An intensive hunting period may have a particularly negative impact on game animals. The aim of this study was to determine the plasma cortisol level and biochemical parameters in 25 wild red deer (Cervus elaphus) harvested during stalking hunts in correlation with the sex and age of the animals. The mean cortisol concentrations in the stags and hinds analyzed in this study were similar (20.2 and 21.5 ng/mL, respectively). Higher HDL cholesterol values were found in the blood of the hinds than in stags (p < 0.05). Similarly, the mean levels of LDL cholesterol, lactate dehydrogenase, and alanine aminotransferase were higher by 21%, 16%, and 42%, respectively, in the blood of the hinds. In contrast, the levels of alkaline phosphatase, bilirubin, and aspartate aminotransferase were higher in the stags (by 30%, 49%, and 36%, respectively). There was a negative correlation of the cortisol concentration with urea and bilirubin and a positive correlation between cortisol and aspartate aminotransferase in the stags (p < 0.05). In turn, a negative correlation was found between the cortisol and urea levels in the hinds (p < 0.05). In summary, the stress caused by stalking hunts and the characteristic behavior of red deer during the mating season had an impact on chosen biochemical parameters. The increased concentration of cortisol resulted in a decrease in the carcass mass, which may lead to the deterioration of the physical condition of animals on hunting grounds.
SWOT and Root Cause Analyses of Antimicrobial Resistance to Oral Antimicrobial Treatment of Cystitis
Authors: Tyagi Pradeep , Tyagi Shachi , Stewart Laurence , Glickman Scott
Published: April 2024

Nearly 150 million cases of urinary tract infections (UTIs) are reported each year, of which uncomplicated cystitis triggers > 25% of outpatient prescriptions of oral antimicrobial treatment (OAT). OAT aids immune cells infiltrating the urothelium in eliminating uropathogens capable of invading the urothelium and surviving hyperosmotic urine. This self-evident adaptability of uropathogens and the short interval between the introduction of Penicillin and the first report of antimicrobial resistance (AMR) implicate AMR as an evolutionary conserved heritable trait of mutant strains selected by the Darwinian principle to survive environmental threats through exponential proliferation. Therefore, AMR can only be countered by antimicrobial stewardship (AMS) following the principle of the five Ds—drug, dose, duration, drug route, and de-escalation. While convenient to administer, the onset of the minimum inhibitory concentration (MIC) for OAT in urine leaves a window of opportunity for uropathogens to survive the first contact with an antimicrobial and arm their descendant colonies with AMR for surviving subsequent higher urine antimicrobial levels. Meanwhile, the initial dose of intravesical antimicrobial treatment (IAT) may be well above the MIC. Therefore, the widespread clinical use of OAT for cystitis warrants an analysis of the strengths, weaknesses, opportunity, and threats (SWOTs) and a root cause analysis of the AMR associated with OAT and IAT.
Analysis of Hybrid Ship Machinery System with Proton Exchange Membrane Fuel Cells and Battery Pack
Authors: Penga Jure , Vidović Tino , Radica Gojmir , Penga Željko
Published: April 2024

As marine traffic is contributing to pollution, and most vessels have predictable routes with repetitive load profiles, to reduce their impact on environment, hybrid systems with proton exchange membrane fuel cells (PEMFC-s) and battery pack are a promising replacement. For this purpose, the new approach takes into consideration an alternative to diesel propulsion with the additional benefit of carbon neutrality and increase of system efficiency. Additionally, in the developed numerical model, control of the PEMFC–battery hybrid energy system with balance of plant is incorporated with repowering existing vessels that have two diesel engines with 300 kWe. The goal of this paper is to develop a numerical model that analyzes and determines an equivalent hybrid ship propulsion system for a known traveling route. The developed numerical model consists of an interconnected system with the PEMFC stack and a battery pack as power sources. The numerical model was developed and optimized to meet the minimal required power demand for a successful route, which has variable loads and sees ships sail daily six times along the same route—in total 54 nautical miles. The results showed that the equivalent hybrid power system consists of a 300 kWe PEMFC stack and battery pack with 424 kWh battery and state of charge varying between 20 and 87%. To power this new hybrid power system, a hydrogen tank of 7200 L holding 284.7 kg at pressure of 700 bar is required, compared to previous system that consumed 1524 kg of diesel and generated 4886 kg of CO2.
YOLO-SAD: An Efficient SAR Aircraft Detection Network
Authors: Chen Junyi , Shen Yanyun , Liang Yinyu , Wang Zhipan , Zhang Qingling
Published: April 2024

Aircraft detection in SAR images of airports remains crucial for continuous ground observation and aviation transportation scheduling in all weather conditions, but low resolution and complex scenes pose unique challenges. Existing methods struggle with accuracy, overlapping detections, and missed targets. We propose You Only Look Once-SAR Aircraft Detector (YOLO-SAD), a novel detector that tackles these issues. YOLO-SAD leverages the Attention-Efficient Layer Aggregation Network-Head (A-ELAN-H) module to prioritize essential features for improved accuracy. Additionally, the SAR Aircraft Detection-Feature Pyramid Network (SAD-FPN) optimizes multi-scale feature fusion, boosting detection speed. Finally, Enhanced Non-Maximum Suppression (EH-NMS) eliminates overlapping detections. On the SAR Aircraft Detection Dataset (SADD), YOLO-SAD achieved 91.9% AP(0.5) and 57.1% AP(0.5:0.95), surpassing the baseline by 2.1% and 1.9%, respectively. Extensive comparisons on SADD further demonstrate YOLO-SAD’s superiority over five state-of-the-art methods in both AP(0.5) and AP(0.5:0.95). The outcomes of further comparative experiments on the SAR-AIRcraft-1.0 dataset confirm the robust generalization capability of YOLO-SAD, demonstrating its potential use in aircraft detection with SAR.
Potential for Biofilm Formation and Antibiotic Resistance of Staphylococci Isolated from Bulk Tank Milk Samples
Authors: Wiszniewska-Łaszczych Agnieszka , Szteyn Joanna , Sołtysiuk Marta , Kowalik Jarosław , Modzelewska-Kapituła Monika
Published: April 2024

The prevalence of staphylococci in the environment of humans, including food, may lead to the production of toxins and food poisoning in consumers. Additionally, staphylococci constitute a reservoir of genes determining antibiotic resistance. The study aimed to compare the antibiotic resistance and biofilm-forming ability of staphylococci isolated from bulk tank milk and assess the occurrence of animal-associated methicillin-resistant Staphylococcus aureus. The study consisted of isolation of strains from cow milk, confirming their belonging to Staphylococcus sp. and S. aureus, investigation of the presence of a gene determining the production of coagulase, and genes determining resistance to β-lactams, tetracyclines, vancomycin, natural penicillins, and aminopenicillins, as well as virulence genes determining biofilm production. There were 56 bacterial strains isolated, which belong to Staphylococcus spp. All isolates showed resistance to amoxicillin, erythromycin, and tetracycline. The lowest resistance was demonstrated for vancomycin (48.2%). The mecA gene was not detected in any of the isolates and there was no positive reaction to the PB2 protein, which indicates that none of the isolated strains was methicillin resistant. Only 26.8% of isolated strains did not show the ability to produce biofilm. Special attention should be paid to the health of dairy cows and the supervision of the use of antibiotics in the dairy herd environment because, even in milk from healthy cows, there is a real risk of the occurrence of multi-drug resistant bacteria.
Impact of Differing Instability Devices on Postural Sway Parameters
Authors: Wallace Kacey M. , Brown Michael R. , Pannell William C. , Daniels Jacob B. , Moore Juliana C. , McInnis Ayden K. , Donahue Paul T. , Garner John C.
Published: April 2024

Single-limb balance training is integral to preventing and rehabilitating lower extremity injuries. Previous investigations have shown that when using instability devices, differences may or may not exist in the postural sway parameters during use, depending on the specific devices being assessed. Thus, this investigation sought to examine the differences between a commonly used foam pad and a novel instability device (block) in postural sway measures. Twenty-two healthy individuals with no history of lower extremity injury or neurological disorders participated in this investigation. The participants performed three single-limb static balance exercises on a force platform sampling at 120 Hz. Each condition contained three 10-s trials separated by thirty seconds. The mean CoP values of the three trials in each condition were then compared using a within-subjects repeated-measures analysis of variance. Statistically significant differences were seen in the sway area between conditions (p = 0.009), the CoP path length (p = 0.007), the peak medial–lateral CoP displacement (p = 0.004), and the average sway velocity (p = 0.007). All variables displayed similar trends whereby the control condition showed significantly lower values than both instability devices, with no differences between devices. This investigation provides supporting evidence that different instability devices may deliver similar changes to postural sway parameters compared to control conditions. The novel block instability device used in this investigation may be used similarly to the traditional foam pad in prevention and rehabilitation settings based on the absence of differences between the two devices.
Enhancing Heartbeat Classification through Cascading Next Generation and Conventional Reservoir Computing
Authors: Arbateni Khaled , Benzaoui Amir
Published: April 2024

Electrocardiography (ECG) is a simple and safe tool for detecting heart conditions. Despite the diaspora of existing heartbeat classifiers, improvements such as real-time heartbeat identification and patient-independent classification persist. Reservoir computing (RC) based heartbeat classifiers are an emerging computational efficiency solution that is potentially recommended for real-time concerns. However, multiclass patient-independent heartbeat classification using RC-based classifiers has not been considered and constitutes a challenge. This study investigates patient-independent heartbeat classification by leveraging traditional RC and next-generation reservoir computing (NG-RC) solely or in a cascade. Three RCs were investigated for classification tasks: a linear RC featuring linear internal nodes, a nonlinear RC with a nonlinear internal node, and an NG-RC. Each of these has been evaluated independently using either linear ridge regression or multilayer perceptron (MLP) as readout models. Only three classes were considered for classification: the N, V, and S categories. Techniques to deal with the imbalanced nature of the data, such as the synthetic minority oversampling technique (SMOTE) and oversampling by replacement, were used. The MIT-BIH dataset was used to evaluate classification performance. The area under the curve (AUC) criterion was used as an evaluation metric. The NG-RC-based model improves classification performance and mitigates the overfitting issue. It has improved classification performance by 4.18% and 2.31% for the intra-patient and inter-patient paradigms, respectively. By cascading RC and NG-RC, the identification performance of the three heartbeat categories is further enhanced. AUCs of 97.80% and 92.09% were reported for intra- and inter-patient scenarios, respectively. These results suggest promising opportunities to leverage RC technology for multiclass, patient-independent heartbeat recognition.
Evaluation of Deviations for Horizontal Thin Walls Determined by Optical and Contact Methods for Milled Samples of Nickel Alloy Inconel 625
Authors: Kurpiel Szymon , Zagórski Krzysztof , Cieślik Jacek , Skrzypkowski Krzysztof , Brostow Witold
Published: April 2024

The aerospace industry is imposing increasingly strict dimensional tolerances, which is forcing continuous development in component manufacturing. Ensuring tight dimensional tolerances is difficult for thin-walled structures due to their reduced stiffness, which are increasingly used in the aerospace industry, where titanium alloys and nickel alloys, among others, dominate. Developments in this area are causing a search for machining conditions that provide sufficient quality characteristics including dimensional and shape accuracy. We discuss, herewith, thin wall deformations in the horizontal orientation of Inconel 625 nickel alloy samples in cross-sections perpendicular and parallel to the direction of tool feed motion. We measured dimensional and shape accuracy using a 3D optical scanner and also using a coordinate measuring machine to correlate these results. We compared the results obtained by the two methods and obtained the maximum discrepancy of the results equal to around 8%. Samples made with adaptive cylindrical milling had similar values of thin wall deviations, with the smallest deviations observed for the sample made with the tool for high-performance machining using adaptive cylindrical milling.
A Systematic Review of Osteotomies to Correct Hallux Valgus in the First Metatarsal
Authors: Santos M. , Roseiro L. , Cortesão Seiça E. , Amaro A. M.
Published: April 2024

Hallux valgus is one of the most common deformities of the forefoot. When addressing hallux valgus, surgical management plays a central role, with the majority of techniques consisting of cutting (osteotomy) and reshaping the overall anatomy of the metatarsal and phalanx. Understanding and analyzing the results of many osteotomies to treat hallux valgus is essential. This systematic review aims to summarize a structure of the different practices used through osteotomies to correct hallux valgus in the metatarsal, the results obtained, and the methodologies used to provide a follow-up. This systematic search was carried out using three databases: the National Library of Medicine, Science Direct, and Sage Journals. This research mainly focused on analyzing the outcomes and post-operative results of the various osteotomies of the first metatarsal manuscripts that addressed more than just the procedure analysis or its results, which were excluded, as in the case of combined pathologies. Fifteen manuscripts were included for full-text analysis based on comparative/explanatory studies of surgical procedures for hallux valgus metatarsal osteotomies and their clinical outcomes. The clinical results were analyzed in two aspects: the dimensional analysis of the metatarsal before and after the surgical procedure and the technologies used to obtain the follow-up. This review will guide future research into the more comprehensive use of the metatarsal osteotomy process and where engineering can and should intervene and make the procedures more precise and straightforward.
Effect of Photobiomodulation Therapy Dosage on Orthodontic Movement, Temporomandibular Dysfunction and Third Molar Surgery Outcomes: A Five-Year Systematic Review
Authors: Parker Steven , Cronshaw Mark , Anagnostaki Eugenia , Mylona Valina , Lynch Edward , Grootveld Martin
Published: April 2024

(1) Background: This five-year systematic review seeks to assess the impact of oral and peri-oral photobiomodulation therapies (PBMTs) on the adjunctive management of deeper tissue biofunction, pathologies related to pain and inflammatory disorders and post-surgical events. (2) Methods: The search engines PubMed, Cochrane, Scopus, ScienceDirect, Google Scholar, EMBASE and EBSCO were used with appropriate Boolean operatives. The initial number of 14,932 articles was reduced to 261. Further exclusions performed to identify PBM therapy in third molar surgery, orthodontic and TMJ articles resulted in 19, 15 and 20 of these, respectively. Each paper was scrutinised to identify visible red–NIR laser wavelength PBM applications, concerning dosimetry and outcomes. (3) Results: A dataset analysis was employed using post hoc ANOVA and linear regression strategies, both with a Bonferroni correction (p < 0.05). The outcomes of articles related to oral surgery pain revealed a statistically significant relation between PBMT and a positive adjunct (p = 0.00625), whereas biofunction stimulation across all other groupings failed to establish a positive association for PBMT. (4) Conclusions: The lack of significance is suggested to be attributable to a lack of operational detail relating to laser operating parameters, together with variation in a consistent clinical technique. The adoption of a consistent parameter recording and the possible inclusion of laser data within ethical approval applications may help to address the shortcomings in the objective benefits of laser PBM.
Modification and Validation of the Soil–Snow Module in the INM RAS Climate Model
Authors: Chernenkov Alexey , Volodin Evgeny , Kostrykin Sergey , Tarasevich Maria , Vorobyeva Vasilisa
Published: April 2024

This paper describes the modification of a simple land snow cover module of the INM RAS climate model. The possible liquid water and refreezing of meltwater in the snow layer are taken into account by the proposed parameterization. This is particularly important for modelling the transition season, as this phenomenon is mainly observed during the formation and melting of the snow cover when the surface temperature fluctuates around 0 °C. The snow density evolution simulation is also added. This parameterization is implemented in the INM-CM snow module and verified on observation data using the ESM-SnowMIP-like protocol. As a result, the INM-CM mean climate snow melt periods are refined, particularly in middle and high latitudes. The snow-covered area according to the model is also improved. In the future, a modified version of the land snow module can be used, coupled with a snow albedo model that takes into account snow metamorphism. This module can also be applied to sea ice snow.
A Heuristic Method for Solving Polynomial Matrix Equations
Authors: González-Santander Juan Luis , Sánchez Lasheras Fernando
Published: April 2024

We propose a heuristic method to solve polynomial matrix equations of the type ∑k=1makXk=B, where ak are scalar coefficients and X and B are square matrices of order n. The method is based on the decomposition of the B matrix as a linear combination of the identity matrix and an idempotent, involutive, or nilpotent matrix. We prove that this decomposition is always possible when n=2. Moreover, in some cases we can compute solutions when we have an infinite number of them (singular solutions). This method has been coded in MATLAB and has been compared to other methods found in the existing literature, such as the diagonalization and the interpolation methods. It turns out that the proposed method is considerably faster than the latter methods. Furthermore, the proposed method can calculate solutions when diagonalization and interpolation methods fail or calculate singular solutions when these methods are not capable of doing so.
Direct Current Stimulation over the Primary Motor Cortex, Cerebellum, and Spinal Cord to Modulate Balance Performance: A Randomized Placebo-Controlled Trial
Authors: Veldema Jitka , Steingräber Teni , von Grönheim Leon , Wienecke Jana , Regel Rieke , Schack Thomas , Schütz Christoph
Published: April 2024

Objectives: Existing applications of non-invasive brain stimulation in the modulation of balance ability are focused on the primary motor cortex (M1). It is conceivable that other brain and spinal cord areas may be comparable or more promising targets in this regard. This study compares transcranial direct current stimulation (tDCS) over (i) the M1, (ii) the cerebellum, and (iii) trans-spinal direct current stimulation (tsDCS) in the modulation of balance ability. Methods: Forty-two sports students were randomized in this placebo-controlled study. Twenty minutes of anodal 1.5 mA t/tsDCS over (i) the M1, (ii) the cerebellum, and (iii) the spinal cord, as well as (iv) sham tDCS were applied to each subject. The Y Balance Test, Single Leg Landing Test, and Single Leg Squat Test were performed prior to and after each intervention. Results: The Y Balance Test showed significant improvement after real stimulation of each region compared to sham stimulation. While tsDCS supported the balance ability of both legs, M1 and cerebellar tDCS supported right leg stand only. No significant differences were found in the Single Leg Landing Test and the Single Leg Squat Test. Conclusions: Our data encourage the application of DCS over the cerebellum and spinal cord (in addition to the M1 region) in supporting balance control. Future research should investigate and compare the effects of different stimulation protocols (anodal or cathodal direct current stimulation (DCS), alternating current stimulation (ACS), high-definition DCS/ACS, closed-loop ACS) over these regions in healthy people and examine the potential of these approaches in the neurorehabilitation.
The Tumor Microenvironment: Signal Transduction
Authors: Zhang Xianhong , Ma Haijun , Gao Yue , Liang Yabing , Du Yitian , Hao Shuailin , Ni Ting
Published: April 2024

In the challenging tumor microenvironment (TME), tumors coexist with diverse stromal cell types. During tumor progression and metastasis, a reciprocal interaction occurs between cancer cells and their environment. These interactions involve ongoing and evolving paracrine and proximal signaling. Intrinsic signal transduction in tumors drives processes such as malignant transformation, epithelial-mesenchymal transition, immune evasion, and tumor cell metastasis. In addition, cancer cells embedded in the tumor microenvironment undergo metabolic reprogramming. Their metabolites, serving as signaling molecules, engage in metabolic communication with diverse matrix components. These metabolites act as direct regulators of carcinogenic pathways, thereby activating signaling cascades that contribute to cancer progression. Hence, gaining insights into the intrinsic signal transduction of tumors and the signaling communication between tumor cells and various matrix components within the tumor microenvironment may reveal novel therapeutic targets. In this review, we initially examine the development of the tumor microenvironment. Subsequently, we delineate the oncogenic signaling pathways within tumor cells and elucidate the reciprocal communication between these pathways and the tumor microenvironment. Finally, we give an overview of the effect of signal transduction within the tumor microenvironment on tumor metabolism and tumor immunity.
The Chicken Egg: An Advanced Material for Tissue Engineering
Authors: Zhang Yuli , Pham Hieu M. , Tran Simon D.
Published: April 2024

The chicken egg, an excellent natural source of proteins, has been an overlooked native biomaterial with remarkable physicochemical, structural, and biological properties. Recently, with significant advances in biomedical engineering, particularly in the development of 3D in vitro platforms, chicken egg materials have increasingly been investigated as biomaterials due to their distinct advantages such as their low cost, availability, easy handling, gelling ability, bioactivity, and provision of a developmentally stimulating environment for cells. In addition, the chicken egg and its by-products can improve tissue engraftment and stimulate angiogenesis, making it particularly attractive for wound healing and tissue engineering applications. Evidence suggests that the egg white (EW), egg yolk (EY), and eggshell membrane (ESM) are great biomaterial candidates for tissue engineering, as their protein composition resembles mammalian extracellular matrix proteins, ideal for cellular attachment, cellular differentiation, proliferation, and survivability. Moreover, eggshell (ES) is considered an excellent calcium resource for generating hydroxyapatite (HA), making it a promising biomaterial for bone regeneration. This review will provide researchers with a concise yet comprehensive understanding of the chicken egg structure, composition, and associated bioactive molecules in each component and introduce up-to-date tissue engineering applications of chicken eggs as biomaterials.
Transient Receptor Potential Canonical 5 (TRPC5): Regulation of Heart Rate and Protection against Pathological Cardiac Hypertrophy
Authors: Thakore Pratish , Clark James E. , Aubdool Aisah A. , Thapa Dibesh , Starr Anna , Fraser Paul A. , Farrell-Dillon Keith , Fernandes Elizabeth S. , McFadzean Ian , Brain Susan D.
Published: April 2024

TRPC5 is a non-selective cation channel that is expressed in cardiomyocytes, but there is a lack of knowledge of its (patho)physiological role in vivo. Here, we examine the role of TRPC5 on cardiac function under basal conditions and during cardiac hypertrophy. Cardiovascular parameters were assessed in wild-type (WT) and global TRPC5 knockout (KO) mice. Despite no difference in blood pressure or activity, heart rate was significantly reduced in TRPC5 KO mice. Echocardiography imaging revealed an increase in stroke volume, but cardiac contractility was unaffected. The reduced heart rate persisted in isolated TRPC5 KO hearts, suggesting changes in basal cardiac pacing. Heart rate was further investigated by evaluating the reflex change following drug-induced pressure changes. The reflex bradycardic response following phenylephrine was greater in TRPC5 KO mice but the tachycardic response to SNP was unchanged, indicating an enhancement in the parasympathetic control of the heart rate. Moreover, the reduction in heart rate to carbachol was greater in isolated TRPC5 KO hearts. To evaluate the role of TRPC5 in cardiac pathology, mice were subjected to abdominal aortic banding (AAB). An exaggerated cardiac hypertrophy response to AAB was observed in TRPC5 KO mice, with an increased expression of hypertrophy markers, fibrosis, reactive oxygen species, and angiogenesis. This study provides novel evidence for a direct effect of TRPC5 on cardiac function. We propose that (1) TRPC5 is required for maintaining heart rate by regulating basal cardiac pacing and in response to pressure lowering, and (2) TRPC5 protects against pathological cardiac hypertrophy.
A Novel Microfluidic Strategy for Efficient Exosome Separation via Thermally Oxidized Non-Uniform Deterministic Lateral Displacement (DLD) Arrays and Dielectrophoresis (DEP) Synergy
Authors: Wang Dayin , Yang Shijia , Wang Ning , Guo Han , Feng Shilun , Luo Yuan , Zhao Jianlong
Published: April 2024

Exosomes, with diameters ranging from 30 to 150 nm, are saucer-shaped extracellular vesicles (EVs) secreted by various type of human cells. They are present in virtually all bodily fluids. Owing to their abundant nucleic acid and protein content, exosomes have emerged as promising biomarkers for noninvasive molecular diagnostics. However, the need for exosome separation purification presents tremendous technical challenges due to their minuscule size. In recent years, microfluidic technology has garnered substantial interest as a promising alternative capable of excellent separation performance, reduced reagent consumption, and lower overall device and operation costs. In this context, we hereby propose a novel microfluidic strategy based on thermally oxidized deterministic lateral displacement (DLD) arrays with tapered shapes to enhance separation performance. We have achieved more than 90% purity in both polystyrene nanoparticle and exosome experiments. The use of thermal oxidation also significantly reduces fabrication complexity by avoiding the use of high-precision lithography. Furthermore, in a simulation model, we attempt to integrate the use of dielectrophoresis (DEP) to overcome the size-based nature of DLD and distinguish particles that are close in size but differ in biochemical compositions (e.g., lipoproteins, exomeres, retroviruses). We believe the proposed strategy heralds a versatile and innovative platform poised to enhance exosome analysis across a spectrum of biochemical applications.
Mapping the Neural Substrates of Cocaine Craving: A Systematic Review
Authors: Paludetto Letícia Silvestri , Florence Luiza Larrubia Alvares , Torales Julio , Ventriglio Antonio , Castaldelli-Maia João Maurício
Published: April 2024

Craving is one of the most important symptoms of cocaine use disorder (CUD) since it contributes to the relapse and persistence of such disorder. This systematic review aimed to investigate which brain regions are modulated during cocaine craving. The articles were obtained through searches in the Google Scholar, Regional BVS Portal, PubMed, and Scielo databases. Overall, there was a selection of 36 studies with 1574 individuals, the majority being participants with CUD, whereby about 61.56% were individuals with CUD and 38.44% were controls (mean age = 40.4 years). Besides the methodological points, the neurobiological investigations comprised fMRI (58.34%) and PET (38.89%). The induction of cocaine craving was studied using different methods: exposure to cocaine cues (69.45%), stressful stimuli, food cues, and methylphenidate. Brain activations demonstrated widespread activity across the frontal, parietal, temporal, and occipital lobes, basal ganglia, diencephalon, brainstem, and the limbic system. In addition to abnormalities in prefrontal cortex activity, abnormalities in various other brain regions’ activity contribute to the elucidation of the neurobiology of cocaine craving. Abnormalities in brain activity are justified not only by the dysfunction of dopaminergic pathways but also of the glutamatergic and noradrenergic pathways, and distinct ways of inducing craving demonstrated the involvement of distinct brain circuits and regions.
Indonesian Stakeholders’ Perspectives on Warning Signs and Beliefs about Suicide
Authors: Setiyawati Diana , Puspakesuma Nabila , Jatmika Wulan Nur , Colucci Erminia
Published: April 2024

The suicide rate in Indonesia is considered low among Asian countries, but the underreporting rate is at a staggering 303%, and the latest reports suggest an increase in suicidal behaviour, particularly among young people. As a multicultural country, Indonesia has a complex system of beliefs about suicide. Thus, various aspects specific to Indonesia must be considered in understanding and preventing suicide. This paper explores Indonesian stakeholders’ perspectives through semi-structured interviews and focus group discussions. A total of 9 participants were individually interviewed, and 42 were involved in focus group discussions. They were mainly people with lived experiences of suicide. The other stakeholders were Indonesian experts who have experience in dealing with suicidal behaviour, helping people with a lived experience of suicide, or were involved in suicide prevention. Indonesian stakeholders highlighted various general and contextualised aspects concerning suicide. These aspects included a wide range of cultural beliefs and culturally specific warning signs, which included “bingung” (confusion) and longing for deceased persons. Other cultural beliefs such as viewing suicide as infectious, unpreventable, and guided by ancient spirits, and as an honourable act in some circumstances, also emerged. These findings can inform suicide prevention programs, including suicide prevention guidelines for Indonesia.
Unconscious Integration of Categorical Relationship of Two Subliminal Numbers in Comparison with “5”
Authors: Li Changjun , Liu Qingying , Liu Yingjuan , Jou Jerwen , Tu Shen
Published: April 2024

Many studies have shown that the brain can process subliminal numerals, i.e., participants can categorize a subliminal number into two categories: greater than 5 or less than 5. In the context of many studies on the unconscious integration of multiple subliminal stimuli, the issue of whether multiple subliminal numbers can be integrated is contentious. The same-different task is regarded as a perfect tool to explore unconscious integration. In the two experiments reported, we used a same-different task in which a pair of masked prime numbers was followed by a pair of target numbers, and participants were asked to decide whether the two target numbers were on the same (both smaller or larger than 5) or different sides (one smaller, the other larger than 5) of 5 in magnitude. The results indicated that the prime numbers could be categorized unconsciously, which was reflected by the category priming effect, and that the unconscious category relationship of the two prime numbers could affect the judgment on the category relationship of the two target numbers, as reflected by the response priming effect. The duration of the prime-to-target interstimulus interval (ISI) was also manipulated, showing a positive compatibility effect (PCE) of category priming and a negative compatibility effect (NCE) of response priming no matter whether the ISI was short (50 ms) or long (150 ms). The NCE, which occurred when the prime-to-target ISI was relatively short in this study, contradicted the conventional view but was consistent with previous results of unconscious integration based on an attention modulation mechanism. Importantly, this study provided evidence for the still-under-debate issue of numerical information integration.
A New Experimental Setup to Characterize Binder–Vegetal Particle Compatibility in Plant-Based Concrete
Authors: Prud’Homme Elodie , Delhomme Fabien , Julliot Clara , Corvalan Loïc , Amziane Sofiane , Toussaint Evelyne , Marceau Sandrine
Published: April 2024

The good insulation properties and the low carbon footprint of vegetal concretes make them promising materials whose use tends to grow continuously. To produce optimized building materials, a better understanding of the interfacial transition zone (ITZ) between vegetal particles and cement paste in terms of the reactions involved and the size of the impacted surface was investigated. This research led to the setting of a reliable visual test to observe ITZ, which enables the monitoring of its appearance and development. Different combinations of vegetal particles and cement pastes were tested to compare the formed ITZ: hemp, rapeseed, and bamboo into Portland and Prompt cement. Finally, a clear link was drawn between the sugar concentration and the size of ITZ. Thanks to image analysis, it was shown that ITZ is due to physico-chemical reactions, with the extraction of free saccharide molecules from the vegetal and water suction followed by their release into the cement paste.
Power Dynamics in Collaborative Governance Processes: A Case Study of a Disadvantaged Neighbourhood in Southern Spain
Authors: Delgado-Baena Antonio , Sianes Antonio
Published: April 2024

This study focuses on assessing collaborative governance from the perspective of power dynamics in a disadvantaged neighbourhood in southern Spain: Las Palmeras. Increasing global urbanisation in recent decades has exacerbated problems of segregation within cities, posing a major challenge to achieving healthy urban environments for harmonious coexistence. Public policies have struggled to address this challenge, particularly following the adoption of the United Nations’ 2030 Agenda for Sustainable Development and its Sustainable Development Goals (SDGs), which have underlined the importance of fostering inclusiveness and sustainability in urban environments. In this context, collaborative governance has emerged as a prominent strategy for democratising the implementation of public policies in urban contexts. However, the efficacy of collaborative governance is hampered by the dynamics of power relations, which limits its effectiveness. The aim of this study is understanding how these dynamics mediate collaborative processes in urban regeneration contexts, an area little addressed so far. Through a case study and the systematisation of experiences, the study proposes strategic recommendations for managing these dynamics within collaborative governance. The insights drawn from this study can serve as a basis for better addressing urban challenges, thus fostering greater inclusiveness and sustainability in urban areas as well as offering valid lines for future research in the field.