bluuebunny/crossref_metadata_2025 huggingface dataset:
The Methodological Quality of Studies on Physical Exercise in Adolescents with Cerebral Palsy: A Scoping Review of Systematic Reviews and Meta-Analyses
Authors: Nitz Alexandrina Cavalcante Rodrigues , Campos Maria João , Antunes Ana Amélia Moraes , da Silva Freitas Emilly , Toscano Chrystiane Vasconcelos Andrade , Ferreira José Pedro
Published: October 2024

Introduction: Systematic reviews and meta-analyses point to the benefits of physical exercise for adolescents with cerebral palsy, improving physical conditioning, muscle strength, balance, and walking speed. However, given the high number of reviews that include randomized and non-randomized studies, it is increasingly necessary to assess the methodological quality of these reviews. This scoping review investigated the methodological quality of systematic reviews and meta-analyses on the effects of physical exercise in adolescents with cerebral palsy to elucidate the methodological limitations of the research and the priorities to be observed in future research. Method: The electronic search used PubMed, Web of Science, and Cochrane. Studies published between 2016 and 2023 were selected. The terms used were “cerebral palsy” combined with “physical fitness”, “exercise”, and “physical activity”. Results: A total of 219 original reviews were selected. Of these, 19 reviews were included for data analysis. AMSTAR2 was used to assess the methodological quality of the reviews. Three reviews presented high methodological quality (15.78%) and three had moderate methodological quality (15.78%). The remaining reviews had low or critically low methodological quality, according to AMSTAR2. Interpretation: This study evidenced that systematic reviews have variable methodological quality and that new studies are still needed.
Factors Influencing Job Satisfaction among Mental Health Nurses: A Systematic Review
Authors: Hudays Ali , Gary Faye , Voss Joachim G. , Arishi Amal , Alfar Zainab A. , Algodimi Ali M. , Fitzpatrick Joyce J.
Published: October 2024

Background/Objectives: Job satisfaction is crucial for healthcare professionals, and understanding its influencing factors is essential for fostering a positive work environment, reducing turnover rates, and improving the overall quality of patient care. This systematic review examined the factors linked to nurse job satisfaction in psychiatric hospitals and diverse psychiatric settings, analyzing the findings through the lens of Herzberg’s theory. Despite existing evidence, gaps remain in understanding the differences in factors affecting job satisfaction across various settings. Methods: Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines, we conducted a comprehensive systematic review by searching six databases, namely PubMed, CINAHL, Cochrane Library, PsycINFO, Scopus, and APA PsycNet. Our search yielded an initial 567 studies published between January 2014 and February 2024, which were subsequently screened and evaluated based on predetermined inclusion criteria. Following this process, a total of 16 studies were deemed eligible for final analysis. Each of these selected studies underwent an independent review by two authors, utilizing the Joanna Briggs Institute checklist tool to ensure rigorous assessment. Results: The findings revealed that interpersonal relationships, working conditions, and recognition were the most frequently reported factors associated with nurse job satisfaction, along with various extrinsic, intrinsic, personal, emotional, and psychosocial factors. Notably, psychiatric hospitals showed a mix of personal, extrinsic, intrinsic, and psychosocial factors influencing job satisfaction, with demographic factors being the most frequently examined. In contrast, diverse psychiatric settings focused more on external aspects impacting job satisfaction. Conclusions: The review highlights the importance of both intrinsic and extrinsic factors and suggests that future research should employ more robust methods and consider psychiatric hospitals and other mental health contexts. Herzberg’s theory provides a valuable framework for understanding the factors associated with nurse job satisfaction.
Progress Achieved, Landmarks, and Future Concerns in Biomedical and Health Informatics
Authors: Ognjanović Ivana , Zoulias Emmanouil , Mantas John
Published: October 2024

Background: The biomedical and health informatics (BMHI) fields have been advancing rapidly, a trend particularly emphasised during the recent COVID-19 pandemic, introducing innovations in BMHI. Over nearly 50 years since its establishment as a scientific discipline, BMHI has encountered several challenges, such as mishaps, delays, failures, and moments of enthusiastic expectations and notable successes. This paper focuses on reviewing the progress made in the BMHI discipline, evaluating key milestones, and discussing future challenges. Methods: To, Structured, step-by-step qualitative methodology was developed and applied, centred on gathering expert opinions and analysing trends from the literature to provide a comprehensive assessment. Experts and pioneers in the BMHI field were assigned thematic tasks based on the research question, providing critical inputs for the thematic analysis. This led to the identification of five key dimensions used to present the findings in the paper: informatics in biomedicine and healthcare, health data in Informatics, nurses in informatics, education and accreditation in health informatics, and ethical, legal, social, and security issues. Results: Each dimension is examined through recently emerging innovations, linking them directly to the future of healthcare, like the role of artificial intelligence, innovative digital health tools, the expansion of telemedicine, and the use of mobile health apps and wearable devices. The new approach of BMHI covers newly introduced clinical needs and approaches like patient-centric, remote monitoring, and precision medicine clinical approaches. Conclusions: These insights offer clear recommendations for improving education and developing experts to advance future innovations. Notably, this narrative review presents a body of knowledge essential for a deep understanding of the BMHI field from a human-centric perspective and, as such, could serve as a reference point for prospective analysis and innovation development.
Use of In-Situ ESR Measurements for Mechanistic Studies of Free Radical Non-Catalytic Thermal Reactions of Various Unconventional Oil Resources and Biomass
Authors: Maqsood Hajra , Abu-Jdayil Basim , Tannous Joy H.
Published: October 2024

The exhaustion of conventional light oils necessitates the shift towards unconventional sources such as biomass, heavy oil, oil shale, and coal. Non-catalytic thermal cracking by a free radical mechanism is at the heart of the upgrading, prior to refining into valuable products. However, thermal pyrolysis is hindered by the formation of asphaltenes, precursors to coke, limiting cracking, causing equipment fouling, and reducing product stability. Free radicals are inherently present in heavy fractions and are generated during thermal processes. This makes these reactive intermediates central to understanding these mechanisms and limiting coking. Electron spin resonance (ESR) spectroscopy facilitates such mechanistic studies. Over the past decade, there has been no review of using in-situ ESR for studying thermal processes. This work begins with a brief description of free radicals’ chain reactions during thermal reactions and the wealth of information ESR provides. We then critically review the literature that uses ESR for mechanistic studies in thermal pyrolysis of biomass, heavy oil, shales, and coal. We conclude that limited literature exist, and more investigations are necessary. The key findings from existing literature are summarized to know the current state of knowledge. We also explicitly highlight the research gaps.
The Avoidance of Purine Stretches by Cancer Mutations
Authors: Vikhorev Aleksandr V. , Savelev Ivan V. , Polesskaya Oksana O. , Rempel Michael M. , Miller Richard A. , Vetcher Alexandre A. , Myakishev-Rempel Max
Published: October 2024

Purine stretches, sequences of adenine (A) and guanine (G) in DNA, play critical roles in binding regulatory protein factors and influence gene expression by affecting DNA folding. This study investigates the relationship between purine stretches and cancer development, considering the aromaticity of purines, quantified by methods like Hückel’s rule and NICS calculations, and the importance of the flanking sequence context. A pronounced avoidance of long purine stretches by typical cancer mutations was observed in public data on the intergenic regions of cancer patients, suggesting a role of intergenic sequences in chromatin reorganization and gene regulation. A statistically significant shortening of purine stretches in cancerous tumors (p value < 0.0001) was found. The insights into the aromatic nature of purines and their stacking energies explain the role of purine stretches in DNA structure, contributing to their role in cancer progression. This research lays the groundwork for understanding the nature of purine stretches, emphasizing their importance in gene regulation and chromatin restructuring, and offers potential avenues for novel cancer therapies and insights into cancer etiology.
Impaired Endothelium-Dependent Vasodilation and Increased Levels of Soluble Fms-like Tyrosine Kinase-1 Induced by Reduced Uterine Perfusion Pressure in Pregnant Rats: Evidence of Protective Effects with Sodium Nitrite Treatment in Preeclampsia
Authors: Da Silva Maria Luiza Santos , Gomes Sáskia Estela Biasotti , Martins Laisla Zanetoni , Rodrigues Serginara David , Toghi Cristal de Jesus , Dias-Junior Carlos Alan
Published: October 2024

Preeclampsia (PE) is a hypertensive disorder of pregnancy and is associated with increases in soluble fms-like tyrosine kinase-1 (sFlt-1) and reductions in nitric oxide (NO) levels. Placental ischemia and hypoxia are hypothesized as initial pathophysiological events of PE. Nitrite (NO metabolite) may be recycled back to NO in ischemic and hypoxic tissues. Therefore, this study examined the sodium nitrite effects in an experimental model of PE. Pregnant rats received saline (Preg group) or sodium nitrite (Preg + Na-Nitrite group). Pregnant rats submitted to the placental ischemia received saline (RUPP group) or sodium nitrite (RUPP + Na-Nitrite group). Blood pressure, placental and fetal weights, and the number of pups were recorded. Plasma levels of NO metabolites and sFlt-1 were also determined. Vascular and endothelial functions were also measured. Blood pressure, placental and fetal weights, the number of pups, NO metabolites, sFlt-1 levels, vascular contraction, and endothelium-dependent vasodilation in the RUPP + Na-Nitrite rats were brought to levels comparable to those in Preg rats. In conclusion, sodium nitrite may counteract the reductions in NO and increases in sFlt-1 levels induced by the placental ischemia model of PE, thus suggesting that increased blood pressure and vascular and endothelial dysfunctions may be attenuated by sodium nitrite-derived NO.
High Ki-67 Expression Predicting a Risk Factor for the Progression of Disease within 24 Months and Microenvironment in Follicular Lymphoma
Authors: Narita Hinako , Kuroiwa Kai , Kawaguchi Yukiko , Murai So , Sasaki Yosuke , Homma Mayumi , Kawamata Natsuki , Hayashi Hidenori , Nagao Kazuki , Okamura Reiko , Uesugi Yuka , Sasaki Yohei , Shimada Shotaro , Watanuki Megumi , Arai Nana , Yanagisawa Kouji , Shiozawa Eisuke , Yamochi Toshiko , Hattori Norimichi
Published: October 2024

Most follicular lymphomas (FLs) demonstrate an indolent clinical course with favorable outcomes; however, a fraction of patients experiences progression of disease within 24 months (POD24) and has adverse outcomes. This study aimed to determine the predictive risk factors for POD24 in patients with FL, and the characteristics of the microenvironment in FL with POD24. By multivariate analysis, we revealed that increased Ki-67 expression was associated with POD24 events in patients with FL (hazard ratio [HR]: 6.29, 95% confidence interval [CI]: 1.96–20.22, p = 0.0020). Additionally, patients with FL with POD24 demonstrated immune cell reduction by immunohistochemistry analysis. Our results help better understand the therapeutic strategies for FL with POD24.
Advances in microRNAs as Emerging Biomarkers for Colorectal Cancer Early Detection and Diagnosis
Authors: Ždralević Maša , Radović Andrijana , Raonić Janja , Popovic Natasa , Klisic Aleksandra , Vučković Ljiljana
Published: October 2024

Colorectal cancer (CRC) remains the second most common cause of cancer-related mortality worldwide, necessitating advancements in early detection and innovative treatment strategies. MicroRNAs (miRNAs), small non-coding RNAs involved in gene regulation, have emerged as crucial players in the pathogenesis of CRC. This review synthesizes the latest findings on miRNA deregulated in precancerous lesions and in CRC. By examining the deregulation patterns of miRNAs across different stages of CRC development, this review highlights their potential as diagnostic tools. We specifically analyse the roles and diagnostic relevance of four miRNAs—miR-15b, miR-21, miR-31, and miR-146a—that consistently exhibit altered expression in CRC. The current knowledge of their role in key oncogenic pathways, drug resistance, and clinical relevance is discussed. Despite challenges posed by the heterogeneity of the research findings on miRNA deregulation and their role in CRC, integrating miRNA diagnostics into current screening methods holds promise for enhancing personalized medicine approaches. This review emphasizes the transformative potential of miRNAs in CRC diagnosis, paving the way for improved patient outcomes and novel therapeutic paradigms.
Impacts of Maternal Preeclampsia Exposure on Offspring Neuronal Development: Recent Insights and Interventional Approaches
Authors: Zhang He , Lin Jinju , Zhao Huashan
Published: October 2024

Preeclampsia, a hypertensive disorder during pregnancy, frequently correlates with adverse neurological outcomes in offspring, including cognitive impairments, autism spectrum disorder, depressive disorder, attention deficit hyperactivity disorder, and cerebral palsy. Despite these known consequences, the understanding of neuronal damage in the offspring of preeclamptic mothers remains insufficient. Here, we review the neuronal abnormalities resulting from maternal preeclampsia exposure, which include disrupted neurogenesis, loss of neuronal cell integrity, accumulation of cellular debris, decreased synaptogenesis and myelination, and increased neurite growth stimulated by maternal preeclampsia serum. The underlying mechanisms potentially driving these effects involve microglial activation, inflammatory responses, and reduced angiogenesis. Intervention strategies aimed at improving fetal neuronal outcomes are also discussed, encompassing pharmacological treatments such as pravastatin, tadalafil, and melatonin, as well as non-pharmacological approaches like dietary modifications, maternal exercise, and standard care for children. These interventions hold promise for clinical application, offering avenues to address early neuronal abnormalities and prevent the onset of long-term neurological disorders.
Genome of Russian Snow-White Chicken Reveals Genetic Features Associated with Adaptations to Cold and Diseases
Authors: Yevshin Ivan S. , Shagimardanova Elena I. , Ryabova Anna S. , Pintus Sergey S. , Kolpakov Fedor A. , Gusev Oleg A.
Published: October 2024

Russian Snow White (RSW) chickens are characterized by high egg production, extreme resistance to low temperatures, disease resistance, and by the snow-white color of the day-old chicks. Studying the genome of this unique chicken breed will reveal its evolutionary history and help to understand the molecular genetic mechanisms underlying the unique characteristics of this breed, which will open new breeding opportunities and support future studies. We have sequenced and made a de novo assembly of the whole RSW genome using deep sequencing (250×) by the short reads. The genome consists of 40 chromosomes with a total length of 1.1 billion nucleotide pairs. Phylogenetic analysis placed the RSW near the White Leghorn, Fayoumi, and Houdan breeds. Comparison with other chicken breeds revealed a wide pool of mutations unique to the RSW. The functional annotation of these mutations showed the adaptation of genes associated with the development of the nervous system, thermoreceptors, purine receptors, and the TGF-beta pathway, probably caused by selection for low temperatures. We also found adaptation of the immune system genes, likely driven by selection for resistance to viral diseases. Integration with previous genome-wide association studies (GWAS) suggested several causal single nucleotide polymorphisms (SNPs). Specifically, we identified an RSW-specific missense mutation in the RALYL gene, presumably causing the snow-white color of the day-old chicks, and an RSW-specific missense mutation in the TLL1 gene, presumably affecting the egg weight.
Environmental Diagnosis through a Flow Cytometric Approach
Authors: Panza Giovanna , Frontalini Fabrizio , Ciacci Caterina , Protano Giuseppe , Montanari Mariele , Lopez Daniele , Nannoni Francesco , Papa Stefano , Ortolani Claudio , Rebecchi Federica , Fusi Vieri , Santolini Riccardo , Canonico Barbara
Published: October 2024

In an era when ecological and environmental needs and responsibilities apply pressure on the world’s countries and sustainability takes centre stage, ecologic/environmental (E/E) laboratories stand as beacons of scientific inquiry, innovating, optimising, and applying various tests for a better knowledge of our natural resources and the quality status of ecosystems. The purpose of this review is to provide an overview of the use of flow cytometry (FC) as a tool for assessing environmental quality, mainly using living organisms and their biological changes as bioindicators. Cytometric approaches applied to both marine and terrestrial ecosystems ensure the detection of biochemical and functional status of the cells composing either an organ thereof or the organism itself. In addition to cytometric evaluations of the biotic matrix, a brief overview of the techniques for the environmental assessment of biotic and abiotic matrices using mass spectrometry is given. The technique involving the continuous monitoring of the chemical and physical parameters of water, sediment, and soil is basically incapable of detecting any additive and synergetic effects of toxicants on living organisms. Therefore, techniques employing bioindicators provide valuable information for environmental diagnosis, and several studies have demonstrated the strong relationship between specific environmental data and cell/organ behaviour.
Transcriptome and Metabolome Reveal Accumulation of Key Metabolites with Medicinal Properties of Phylloporia pulla
Authors: Jiang Ji-Hang , Li Qian-Zhu , Luo Xing , Yu Jia , Zhou Li-Wei
Published: October 2024

Phylloporia pulla, a macrofungal species in the Hymenochaetales, Basidiomycota, is known to enhance the nutritional and bioactive properties of rice through co-fermentation; however, its own secondary metabolites are not well understood. In this study, an integrative analysis of transcriptome and metabolome data revealed that the accumulation of steroids, steroid derivatives, and triterpenoids in P. pulla peaks during the mid-growth stage, while the genes associated with these metabolites show higher expression levels from the early to mid-growth stages. Weighted gene co-expression network analysis identified several modules containing candidate genes involved in the synthesis of steroids, steroid derivatives, and triterpenoids. Specifically, six key hub genes were identified, along with their connectivity to other related genes, as potential catalysts in converting the precursor lanosterol to celastrol. This study enhances our understanding of the secondary metabolites of P. pulla and is essential for the selective utilization of these bioactive compounds.
Multigene Panel Next-Generation Sequencing Techniques in the Management of Patients with Metastatic Colorectal Carcinoma: The Way Forward for Personalized Treatment? A Single-Center Experience
Authors: Matteucci Laura , Sullo Francesco Giulio , Gallio Chiara , Esposito Luca , Muratore Margherita , Rapposelli Ilario Giovanni , Calistri Daniele , Petracci Elisabetta , Rengucci Claudia , Capelli Laura , Chiadini Elisa , Ulivi Paola , Passardi Alessandro , Bittoni Alessandro
Published: October 2024

The efficacy and cost-effectiveness of Multigene Panel Next-Generation Sequencing (NGS) in directing patients towards genomically matched therapies remain uncertain. This study investigated metastatic colorectal cancer (mCRC) patients who underwent NGS analysis on formalin-fixed paraffin-embedded tumor samples. Data from 179 patients were analyzed, revealing no mutations in 39 patients (21.8%), one mutation in 83 patients (46.4%), and two or more mutations in 57 patients (31.8%). KRAS mutations were found in 87 patients (48.6%), including KRAS G12C mutations in 5 patients (2.8%), PIK3CA mutations in 40 patients (22.4%), and BRAF mutations in 26 patients (14.5%). Less common mutations were identified: ERBB2 in five patients (2.8%) and SMO in four patients (2.2%). Additionally, MAP2K1, CTNNB1, and MYC were mutated in three patients (2.4%). Two mutations (1.1%) were observed in ERBB3, RAF1, MTOR, JAK1, and FGFR2. No significant survival differences were observed based on number of mutations. In total, 40% of patients had druggable molecular alterations, but only 1.1% received genomically guided treatment, suggesting limited application in standard practice. Despite this, expanded gene panel testing can identify actionable mutations, aiding personalized treatment strategies in metastatic CRC, although current eligibility for biomarker-guided trials remains limited.
Therapeutic Potential of Intermittent Hypoxia in Atrial Fibrillation
Authors: Park Hyewon , Park Bokyeong , Kim Kyu-sung , Son Young Hoon , Park Sung Jin , Lee Kichang , Park Hyelim , Park Junbeom
Published: October 2024

Intermittent hypoxia (IH) has been extensively studied in recent years, demonstrating adverse and beneficial effects on several physiological systems. However, the precise mechanism underlying its cardiac effects on the heart remains unclear. This study aims to explore the effect of treatment on atrial fibrillation under IH conditions, providing data that can potentially be used in the treatment of heart disease. An atrial fibrillation (AF) model was induced by injecting monocrotaline (MCT, 60 mg/kg) into rats. The study included 32 rats divided into four groups: Control, Control + IH, AF, and AF + IH. We evaluated molecular changes associated with AF using ELISA and Western blot and performed electrophysiological experiments to evaluate AF. Arrhythmia-related calcium and fibrosis markers were investigated. Phosphorylation levels of CaMKII, Phospholamban, and RyR2 all increased in the AF group but decreased in the IH-exposed group. Additionally, fibrosis marker expressions such as SMA, MMP2, MMP9, and TGF-β increased in the AF group but were significantly downregulated with IH treatment. Connexin 43 and AQP4 expression were restored in the IH-treated group. These findings suggest that IH may prevent AF by downregulating the expression of calcium-handling proteins and fibrosis-associated proteins in an AF-induced rat model.
Synthesis and Characterization of Extremely Bulky Aminopyridinate Ligands and a Series of Their Groups 1 and 2 Metal Complexes
Authors: Earsad Arif M. , Paparo Albert , Evans Matthew J. , Jones Cameron
Published: October 2024

High-yielding synthetic routes to five new extremely bulky aminopyridine pro-ligands were developed, viz. (C5H3N-6-Ar1)N(H)Ar2-2; Ar1 = Trip, Ar2 = TCHP (HAmPy1), Ar* (HAmPy2) or Ar† (HAmPy3); Ar1 = TCHP, Ar2 = Ar* (HAmPy4) or Ar† (HAmPy5) (Trip = 2,4,6-triisopropylphenyl, TCHP = 2,4,6-tricyclohexylphenyl, Ar* = C6H2(CHPh2)2Me-2,6,4, Ar† = C6H2(CHPh2)2Pri-2,6,4. Four of these were deprotonated with LiBun in diethyl ether to give lithium aminopyridinate complexes which were dimeric for the least bulky ligand, [{Li(AmPy1)}2] or monomeric for the bulkier aminopyridinates, i.e., in [Li(AmPy2−4)(OEt2)]. One aminopyridine was deprotonated with MeMgI to give monomeric [Mg(AmPy3)I(OEt2)2]. When treated with sodium or potassium mirrors or 5% w/w Na/NaCl, over-reduction occurred, leading to the alkali metal aminopyridinates, [M(AmPy3)(η6-toluene)] (M = Na or K) or [{Na(AmPy3)}∞]. An attempted reduction of [Mg(AmPy3)I(OEt2)2] with a dimagnesium(I) compound led only to partial loss of diethyl ether and the formation of [(AmPy3)Mg(μ-I)2Mg(AmPy3)(OEt2)]. All prepared complexes have potential as ligand transfer reagents in salt metathesis reactions with metal halide complexes.
Soil Bacteria from the Namib Desert: Insights into Plant Growth Promotion and Osmotolerance in a Hyper-Arid Environment
Authors: Lopes Tiago , Santos Jacinta , Matos Diana , Sá Carina , Pina Diogo , Pinto Ricardo , Cardoso Paulo , Figueira Etelvina
Published: October 2024

The Namib Desert is characterized by a number of abiotic stresses, including high temperature, high salinity, osmotic pressure, alkaline pH, and limited water availability. In such environments, dry soils typically exhibit a low water potential, scarce nutrients, and high concentrations of dissolved ions, collectively creating a challenging habitat for microbial life. In this study, 89 bacterial isolates belonging to 20 genera were identified. Bacteria demonstrated significant osmotolerance, with some strains thriving at polyethylene glycol (PEG) concentrations exceeding 20%. Furthermore, these bacteria demonstrated halotolerance, high pH tolerance, and capacity to produce plant growth-promoting (PGP) traits under conditions of osmotic stress. Osmotolerant bacteria exhibited higher proficiency in siderophore production, potassium solubilization, and phosphorus solubilization, all of which are critical for supporting plant growth in nutrient-scarce and stressful environments, such as deserts. However, alginate production was higher in isolates that were less osmotolerant, indicating the potential for a compensatory mechanism in strains that were more sensitive. These findings highlight the complex strategies employed by desert bacteria to survive and support host plants in extreme environments. The present study not only enhances our understanding of microbial adaptations in arid ecosystems, but also provides important information for the development of potential applications for these bacteria in the reclamation of arid land and agricultural practices aimed at improving crop resilience to abiotic stress.
Moderate Sedation or Deep Sedation for ERCP: What Are the Preferences in the Literature?
Authors: Melita Giuseppinella , Tripodi Vincenzo Francesco , Pallio Socrate , Shahini Endrit , Vitello Alessandro , Sinagra Emanuele , Facciorusso Antonio , Mazzeo Anna Teresa , Choudhury Arup , Dhar Jahnvi , Samanta Jayanta , Maida Marcello Fabio
Published: October 2024

One of the most essential procedures for individuals with biliopancreatic disorders is endoscopic retrograde cholangiopancreatography (ERCP). It is based on the combination of endoscopy and radiology to study the biliopancreatic ducts and apply therapeutic solutions. ERCP is currently used to treat choledocholithiasis with or without cholangitis, as well as pancreatic duct stones, benign bile, and pancreatic leaks. On the other hand, ERCP is an unpleasant procedure that must be conducted under anesthetic (moderate sedation, deep sedation, or general anesthesia). With procedures becoming more challenging, the role of anesthesia in ERCP has become even more relevant, and the use of general anesthesia has become better defined. In the last decades, many drugs have been used and some new drugs, like dexmedetomidine, have been recently introduced for sedation or anesthesia during ERCP. Moreover, the scientific community is still divided on the level of sedation to be applied, as well as on appropriate airway management. We therefore performed a narrative review of the literature to assess currently available anesthetic medications for elective ERCP and evidence supporting their effectiveness.
On the Roles of Protein Intrinsic Disorder in the Origin of Life and Evolution
Authors: Uversky Vladimir N.
Published: October 2024

Obviously, the discussion of different factors that could have contributed to the origin of life and evolution is clear speculation, since there is no way of checking the validity of most of the related hypotheses in practice, as the corresponding events not only already happened, but took place in a very distant past. However, there are a few undisputable facts that are present at the moment, such as the existence of a wide variety of living forms and the abundant presence of intrinsically disordered proteins (IDPs) or hybrid proteins containing ordered domains and intrinsically disordered regions (IDRs) in all living forms. Since it seems that the currently existing living forms originated from a common ancestor, their variety is a result of evolution. Therefore, one could ask a logical question of what role(s) the structureless and highly dynamic but vastly abundant and multifunctional IDPs/IDRs might have in evolution. This study represents an attempt to consider various ideas pertaining to the potential roles of protein intrinsic disorder in the origin of life and evolution.
Formation and Mechano-Chemical Properties of Chromium Fluorides Originated from the Deposition of Carbon-Chromium Nanocomposite Coatings in the Reactive Atmosphere (Ar + CF4) during Magnetron Sputtering
Authors: Roślak Adam , Doering Józef , Strzałka Wioletta , Makówka Marcin , Jędrzejczak Anna , Kołodziejczyk Łukasz , Balcerzak Jacek , Jóźwiak Łukasz , Piwoński Ireneusz , Pawlak Wojciech
Published: October 2024

The literature analysis did not indicate any studies on fluorination tests of carbon nanocomposite coatings doped with transition metals in a form of nanocrystalline metal carbide in amorphous carbon matrix (nc-MeC/a-C). As a model coating to investigate the effect of fluorination in a tetrafluoromethane (CF4) atmosphere, a nanocomposite carbon coating doped with chromium-forming nanocrystals of chromium carbides in a-C matrix (nc-CrC/a-C) produced by magnetron sputtering from graphite targets and using a Pulse-DC type medium frequency power supply was chosen. After the deposition of the gradient chromium carbonitride (CrCN) adhesive sublayer, the fluorination of the main coating was conducted in a reactive mode in an (Ar + CF4) atmosphere at various CF4 content. It was observed that the presence of CF4 in the atmosphere resulted in a reduced amount of chromium carbides formed in favor of chromium fluorides. Thus far, this is an observation that seems unnoticed by the carbon coatings researchers. Fluorine was assumed to bond much more readily to carbon than to chromium, due to the stability of tetrafluoromethane (CF4). The opposite seems to be true. The mechanical properties (nano-hardness and Young’s modulus) and tribological properties in the ‘pin-on-disc’ friction pair are presented, along with the analysis of bonds occurring between chromium, carbon, and fluorine by means of X-ray photoelectron spectroscopy (XPS).
Dynamic Behavior and Energy Absorption of Typical Porous Materials under Impacts
Authors: Xie Kui , Li Menglong , Shen Jianghua
Published: October 2024

Porous materials are known for their excellent energy absorption capability and, thus, are widely used in anti-impact applications. However, how the pore shape and size impact the failure mechanism and overall behavior of the porous materials under impact loading is still unclear or limitedly touched. Instead of using homogeneous solids for the porous material model, pores with various shapes and sizes were implanted in a solid to establish the porous materials that have true porous structures, which permits exploration of the local failure mechanism. The results revealed that differently shaped holes have two different dominant deformation modes. And due to their different local stress distributions, they enter the plastic phase earlier and, thus, have higher specific energy absorption. Meanwhile, the model changes from hardening to a quasi-zero stiffness model as the hole size increases. The application of this work can be extended into the field of impact resistance.
Microstructural and Performance Analysis of (TiAl)95−xCu5Nix Coatings Prepared via Laser Surface Cladding on Ti–6Al–4V Substrates
Authors: Jia Wenchang , Song Xiaojie , Zhu Yuming , Jiang Di , Liu Minglei , Ji Yupeng , Zhou Dazhou , Wang Yi
Published: October 2024

In this study, the surface of (Ti-6Al-4V)TC4 alloy was modified via laser cladding. The elemental composition of the coating was (TiAl)95−xCu5Nix, with Ni as the variable (where x = 0, 3, 6, and 9 at.%). Multi-principal alloy coatings were successfully prepared, and their constituent phases, microstructures, and chemical compositions were thoroughly investigated. The hardness and wear resistance of the coatings were analyzed, and the compositions and interfacial characteristics of the different phases were examined via transmission electron microscopy. The analysis revealed that Ni formed a solid solution and a eutectic structure in the Ti(Al, Cu)2 phase. These findings provide valuable insights into the coating properties. Moreover, reciprocal dry sliding friction experiments were conducted to investigate the wear mechanism. The results revealed a significant increase in wear resistance owing to the formation of a Ni solid solution and changes in the coating structure. Additionally, tensile tests demonstrated that the tensile strength of the coatings initially increased and then decreased with varying Ni content. By combining these results with various analyses, we determined that the coating exhibited optimal properties at a Ni content of 6 at.%. Overall, this study comprehensively investigated the microstructure and phase transition behavior of these coatings through various analytical techniques. These findings provide valuable guidance for further optimizing both the preparation process and the performance of the coatings. The coatings exhibit excellent wear resistance and could inspire the design of more advanced protective surfaces.
Progresses on Some Open Problems Related to Infinitely Many Symmetries
Authors: Lou Senyue
Published: October 2024

The quest to reveal the physical essence of the infinitely many symmetries and/or conservation laws that are intrinsic to integrable systems has historically posed a significant challenge at the confluence of physics and mathematics. This scholarly investigation delves into five open problems related to these boundless symmetries within integrable systems by scrutinizing their multi-wave solutions, employing a fresh analytical methodology. For a specified integrable system, there exist various categories of n-wave solutions, such as the n-soliton solutions, multiple breathers, complexitons, and the n-periodic wave solutions (the algebro-geometric solutions with genus n), wherein n denotes an arbitrary integer that can potentially approach infinity. Each subwave comprising the n-wave solution may possess free parameters, including center parameters ci, width parameters (wave number) ki, and periodic parameters (the Riemann parameters) mi. It is evident that these solutions are translation invariant with respect to all these free parameters. We postulate that the entirety of the recognized infinitely many symmetries merely constitute linear combinations of these finite wave parameter translation symmetries. This conjecture appears to hold true for all integrable systems with n-wave solutions. The conjecture intimates that the currently known infinitely many symmetries is not exhaustive, and an indeterminate number of symmetries remain to be discovered. This conjecture further indicates that by imposing an infinite array of symmetry constraints, it becomes feasible to derive exact multi-wave solutions. By considering the renowned Korteweg–de Vries (KdV) equation and the Burgers equation as simple examples, the conjecture is substantiated for the n-soliton solutions. It is unequivocal that any linear combination of the wave parameter translation symmetries retains its status as a symmetry associated with the particular solution. This observation suggests that by introducing a ren-variable and a ren-symmetric derivative, which serve as generalizations of the Grassmann variable and the super derivative, it may be feasible to unify classical integrable systems, supersymmetric integrable systems, and ren-symmetric integrable systems within a cohesive hierarchical framework. Notably, a ren-symmetric integrable Burgers hierarchy is explicitly derived. Both the supersymmetric and the classical integrable hierarchies are encompassed within the ren-symmetric integrable hierarchy. The results of this paper will make further progresses in nonlinear science: to find more infinitely many symmetries, to establish novel methods to solve nonlinear systems via symmetries, to find more novel exact solutions and new physics, and to open novel integrable theories such as the ren-symmetric integrable systems and the possible relations to fractional integrable systems.
Enhanced Electrochemiluminescence of Luminol and-Dissolved Oxygen by Nanochannel-Confined Au Nanomaterials for Sensitive Immunoassay of Carcinoembryonic Antigen
Authors: Li Weibin , Yu Ruliang , Xi Fengna
Published: October 2024

Simple development of an electrochemiluminescence (ECL) immunosensor for convenient detection of tumor biomarker is of great significance for early cancer diagnosis, treatment evaluation, and improving patient survival rates and quality of life. In this work, an immunosensor is demonstrated based on an enhanced ECL signal boosted by nanochannel-confined Au nanomaterial, which enables sensitive detection of the tumor biomarker—carcinoembryonic antigen (CEA). Vertically-ordered mesoporous silica film (VMSF) with a nanochannel array and amine groups was rapidly grown on a simple and low-cost indium tin oxide (ITO) electrode using the electrochemically assisted self-assembly (EASA) method. Au nanomaterials were confined in situ on the VMSF through electrodeposition, which catalyzed both the conversion of dissolved oxygen (O2) to reactive oxygen species (ROS) and the oxidation of a luminol emitter and improved the electrode active surface. The ECL signal was enhanced fivefold after Au nanomaterial deposition. The recognitive interface was fabricated by covalent immobilization of the CEA antibody on the outer surface of the VMSF, followed with the blocking of non-specific binding sites. In the presence of CEA, the formed immunocomplex reduced the diffusion of the luminol emitter, resulting in the reduction of the ECL signal. Based on this mechanism, the constructed immunosensor was able to provide sensitive detection of CEA ranging from 1 pg·mL−1 to 100 ng·mL−1 with a low limit of detection (LOD, 0.37 pg·mL−1, S/N = 3). The developed immunosensor exhibited high selectivity and good stability. ECL determination of CEA in fetal bovine serum was achieved.
Overview of Venous Thromboembolism and Emerging Therapeutic Technologies Based on Nanocarriers-Mediated Drug Delivery Systems
Authors: Salavati Masoud , Arabshomali Arman , Nouranian Sasan , Shariat-Madar Zia
Published: October 2024

Venous thromboembolism (VTE) is a serious health condition and represents an important cause of morbidity and, in some cases, mortality due to the lack of effective treatment options. According to the Centers for Disease Control and Prevention, 3 out of 10 people with VTE will have recurrence of a clotting event within ten years, presenting a significant unmet medical need. For some VTE patients, symptoms can last longer and have a higher than average risk of serious complications; in contrast, others may experience complications arising from insufficient therapies. People with VTE are initially treated with anticoagulants to prevent conditions such as stroke and to reduce the recurrence of VTE. However, thrombolytic therapy is used for people with pulmonary embolism (PE) experiencing low blood pressure or in severe cases of DVT. New drugs are under development, with the aim to ensure they are safe and effective, and may provide an additional option for the treatment of VTE. In this review, we summarize all ongoing trials evaluating anticoagulant interventions in VTE listed in clinicaltrials.gov, clarifying their underlying mechanisms and evaluating whether they prevent the progression of DVT to PE and recurrence of thrombosis. Moreover, this review summarizes the available evidence that supports the use of antiplatelet therapy for VTE. Since thrombolytic agents would cause off-target effects, targeted drug delivery platforms are used to develop various therapeutics for thrombotic diseases. We discuss the recent advances achieved with thrombus-targeting nanocarriers as well as the major challenges associated with the use of nanoparticle-based therapeutics.
Multi-Wavelength Excitable Multicolor Upconversion and Ratiometric Luminescence Thermometry of Yb3+/Er3+ Co-Doped NaYGeO4 Microcrystals
Authors: Zeng Hui , Wang Yangbo , Zhang Xiaoyi , Bu Xiangbing , Liu Zongyi , Li Huaiyong
Published: October 2024

Excitation wavelength controllable lanthanide upconversion allows for real-time manipulation of luminescent color in a composition-fixed material, which has been proven to be conducive to a variety of applications, such as optical anti-counterfeiting and information security. However, current available materials highly rely on the elaborate core–shell structure in order to ensure efficient excitation-dependent energy transfer routes. Herein, multicolor upconversion luminescence in response to both near-infrared I and near-infrared II (NIR-I and NIR-II) excitations is realized in a novel but simple NaYGeO4:Yb3+/Er3+ phosphor. The remarkably enhanced red emission ratio under 1532 nm excitation, compared with that under 980 nm excitation, could be attributed to the Yb3+-mediated cross-relaxation energy transfers. Moreover, multi-wavelength excitable temperature-dependent (295–823 K) upconversion luminescence realizes a ratiometric thermometry relying on the thermally coupled levels (TCLs) of Er3+. Detailed investigations demonstrate that changing excitation wavelength makes little difference for the performances of TCL-based ratiometric thermometry of NaYGeO4:Yb3+/Er3+. These findings gain more insights to manipulate cross-relaxations for excitation controllable upconversion in single activator doped materials and benefit the cognition of the effect of excitation wavelength on ratiometric luminescence thermometry.
Unveiling New Horizons: Advancing Technologies in Cosmeceuticals for Anti-Aging Solutions
Authors: Alves Patrícia Lius Melo , Nieri Vitor , Moreli Fernanda de Campos , Constantino Ederson , de Souza Jocimar , Oshima-Franco Yoko , Grotto Denise
Published: October 2024

In the last years, the landscape of anti-aging cosmetics has been marked by significant advances in cosmeceutical delivery systems. This study aimed to conduct a systematic review of these technological innovations, with a focus on anti-aging effects, from 2018 to 2023. The methodology included a thorough search on PubMed and through gray literature, applying rigorous exclusion criteria. The descriptors were selected based on the Medical Subject Headings (MeSH). A total of 265 articles were found. Exclusion criteria were applied, and 90 of them were selected for full reading. After reading the full 90 articles, 52 were excluded, leaving 38 articles for final evaluation composing this review. The key findings highlighted a clear prevalence of studies exploring nanotechnology, including nanoparticles, niosomes, and liposomes. Most of the formulations analyzed in this review emphasize antioxidant activities, which play a crucial role in preventing premature aging caused by free radicals. The reviewed studies revealed specific activities, such as the reduction in melanin synthesis, the inhibition of enzymes involved in the skin aging process, and the prevention of morphological changes typical of aging.
Functional Nanomaterials for Sensing Devices: Synthesis, Characterization and Applications
Authors: Vercelli Barbara
Published: October 2024

The growing field of nanotechnology impacts many research areas, such as engineering, electronics, energy, environment, biology, and medicine [...]
Development and Biomedical Application of Non-Noble Metal Nanomaterials in SERS
Authors: Chen Liping , Liu Hao , Gao Jiacheng , Wang Jiaxuan , Jin Zhihan , Lv Ming , Yan Shancheng
Published: October 2024

Surface-enhanced Raman scattering (SERS) is vital in many fields because of its high sensitivity, fast response, and fingerprint effect. The surface-enhanced Raman mechanisms are generally electromagnetic enhancement (EM), which is mainly based on noble metals (Au, Ag, etc.), and chemical enhancement (CM). With more and more studies on CM mechanism in recent years, non-noble metal nanomaterial SERS substrates gradually became widely researched and applied due to their superior economy, stability, selectivity, and biocompatibility compared to noble metal. In addition, non-noble metal substrates also provide an ideal new platform for SERS technology to probe the mechanism of biomolecules. In this paper, we review the applications of non-noble metal nanomaterials in SERS detection for biomedical engineering in recent years. Firstly, we introduce the development of some more common non-noble metal SERS substrates and discuss their properties and enhancement mechanisms. Subsequently, we focus on the progress of the application of SERS detection of non-noble metal nanomaterials, such as analysis of biomarkers and the detection of some contaminants. Finally, we look forward to the future research process of non-noble metal substrate nanomaterials for biomedicine, which may draw more attention to the biosensor applications of non-noble metal nanomaterial-based SERS substrates.
Genome-Wide Identification of the RALF Gene Family and Expression Pattern Analysis in Zea mays (L.) under Abiotic Stresses
Authors: Xue Baoping , Liang Zicong , Liu Yue , Li Dongyang , Liu Chang
Published: October 2024

Rapid Alkalization Factor (RALF) is a signaling molecule in plants that plays a crucial role in growth and development, reproductive processes, and responses to both biotic and abiotic stresses. Although RALF peptides have been characterized in Arabidopsis and rice, a comprehensive bioinformatics analysis of the ZmRALF gene family in maize is still lacking. In this study, we identified 20 RALF genes in the maize genome. Sequence alignment revealed significant structural variation among the ZmRALF family genes. Phylogenetic analysis indicates that RALF proteins from Arabidopsis, rice, and maize can be classified into four distinct clades. Duplication events suggest that the expansion of the RALF gene family in maize primarily relies on whole-genome duplication. ZmRALF genes are widely expressed across various tissues; ZmRALF1/15/18/19 are highly expressed in roots, while ZmRALF6/11/14/16 are predominantly expressed in anthers. RNA-seq and RT-qPCR demonstrated that the expression levels of ZmRALF7, ZmRALF9, and ZmRALF13 were significantly up-regulated and down-regulated in response to PEG and NaCl stresses, respectively. Overall, our study provides new insights into the role of the RALF gene family in abiotic stress.