bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Cases of Burkitt Lymphoma Presenting as Solid Ovarian Masses
Authors: Ngam Blessing N. , Brown James A. , Elit Lorraine , Bardin Richard L , Snell Mark J.
Published: May 2023

Background: Endemic Burkitt lymphoma has been associated with the Epstein Barr Virus (EBV), particularly in malaria-endemic regions. Primary ovarian Burkitt is an infrequent entity of this disease, and its diagnosis still poses a challenge.

Summary: We present two cases. The first case is that of a 23-year-old female G1P1 who presented with a two-week history of abdominal pain and progressive distension. Her examination was remarkable for two pelvic masses and signs of ascites. An ultrasound scan showed bilateral solid adnexal masses, ascites, and mild splenomegaly. Tumor markers for ovarian and germ cell tumors were within the normal ranges. Cytology on ascitic fluid was negative for malignancy. Lactate dehydrogenase (LDH) level and further imaging were not done due to financial constraints. With the working diagnosis of an ovarian epithelial malignancy versus drop metastases, she underwent exploratory laparotomy, total abdominal hysterectomy, bilateral salpingo-oophorectomy, appendectomy, omentectomy, and debulking of peritoneal implants. Histopathology two weeks after surgery showed Burkitt lymphoma. The second case is that of a 12-year old female whose management averted surgery, following the recommendations made from our first case.

Conclusion: Lymphomas are amongst the most common tumors in the pediatric age group. Clinicians should maintain a high index of suspicion in the face of fast-growing tumors. Burkitt lymphomas respond well to chemotherapy, and surgery is seldom necessary except for life-threatening presentations. Management of oncologic cases that are not straightforward should follow a multidisciplinary approach.
Fear and Anxiety among Dental Professionals to Combat Novel Coronovirus Disease (COVID-19) Outbreak in Mumbai city
Authors: Rodrigues Silvia , Patil Shruti , Shah Rohit , Ghangrekar Kashmira , Lakade Chaitrali
Published: May 2023

Objective:
Originating in China in December 2019, coronavirus disease 2019 (COVID-19) rapidly spread to more than 216 countries in the world by May 2020. Because dentists are at a higher risk of contracting the disease, the present study assessed the fear and anxiety among dental practitioners of becoming infected with COVID-19.

Methods:
An online cross-sectional questionnaire survey comprising 14 questions was conducted among dental practitioners of Mumbai. Age, gender,highest qualification, type of practice, years of practice, and place of residence were the demographic variables were recorded. It was a cross-sectional questionnaire-based study carried out among the dental professionals by using a 5-point Like rt scale to record the responses of study participants. Comparison of the mean score was done using t-test for 2 variables and analysis of variance for 3 or more than 3 variables. Multiple logistic regression analysis of the levels of fear with demographic variables was done. P < 0.05 was considered statistically significant.

Results:
Conclusions: The present study demonstrates cross-sectional data of fear and anxiety among dental practitioners during the COVID-19 outbreak. Heightened levels of fear observed in this study call for a nationwide analysisoffearamongdentistsanddeliberatemanagementstrategiesforthesame.
Key Words: anxiety, COVID-19, dental practitioners, fear, Mumbai, pandemic
Impacts of COVID-19 on alcohol use among help-seeking adults
Authors: Srinagesh Aradhana , Forthal Sarah , Madden Sean P. , Stein L. A. R. , Muench Frederick
Published: May 2023

The coronavirus (COVID-19) pandemic has been associated with both increased and decreased alcohol use. Authors explored reasons for increased and decreased alcohol use since the COVID-19 lockdown (March 2020) in a sample of help-seeking adults (HSA) participating in a remote-based alcohol reduction text-messaging intervention in the USA. At the time of recruitment, the HSA in this study were interested in reducing rather than stopping their alcohol consumption. An optional self-report questionnaire was completed by 324 participants (mean age 41.6 ± 10.2 years; 71.5% female; 83.9% White) in February 2021. Survey questions assessed sociodemographic factors, social stressors (quarantine conditions, employment status, changes to daily routine), and drinking patterns. Authors fit two ordinal logistic regression models: one for increased drinking and one for decreased drinking, as functions of the potential predictors and control variables. Most participants (n = 281; 87.0%) reported drinking more than usual since COVID-19 lockdown began. The most common self-reported reasons for drinking more were increased stress/anxiety (74.7%), boredom (69.4%), and spending more time at home (65.5%) whereas reasons for drinking less were less socializing (33.7%) and worrying about how alcohol would impact the immune system (31.5%). Identifying as female, severity of changes to daily routine, and increased access to alcohol were significantly associated with drinking more than usual. These data suggest that the general consequences of the pandemic in the general population (e.g., boredom) led to greater alcohol use among help-seeking adults attempting to reduce their drinking. Identifying these factors may help create more targeted interventions during public health crises.
aMplitude spectral area of ventricular fibrillation and amiOdarone Study in patients with out-of-hospital cArdIaC arrest. The MOSAIC study
Authors: Gentile Francesca Romana , Wik Lars , Aramendi Elisabete , Baldi Enrico , Isasi Iraia , Steen-Hansen Jon Erik , Compagnoni Sara , Fasolino Alessandro , Contri Enrico , Palo Alessandra , Primi Roberto , Bendotti Sara , Currao Alessia , Savastano Simone
Published: May 2023

Objective

Antiarrhythmic drugs are recommended for out of hospital cardiac arrest (OHCA) with shock-refractory ventricular fibrillation (VF). Amplitude Spectral Area (AMSA) of VF is a quantitative waveform measure that describes the amplitude-weighted mean frequency of VF, it correlates with intramyocardial adenosine triphosphate (ATP) concentration, it is a predictor of shock efficacy and an emerging indicator to guide defibrillation and resuscitation efforts. How AMSA might be influenced by amiodarone administration is unknown.

Methods

In this international multicentre observational study, all OHCAs receiving at least one shock were included. AMSA values were calculated by retrospectively analysing the pre-shock ECG interval of 2 s. Multivariable models were run and a propensity score based on the probability of receiving amiodarone was created to compare two randomly matched samples.

Results

2,077 shocks were included: 1,407 in the amiodarone group and 670 in the non-amiodarone group. AMSA values were lower in the amiodarone group [8.8 (6–12.7) mV·Hz vs. 9.8 (6–14) mV·Hz, p = 0.035]. In two randomly matched propensity score-based groups of 261 shocks, AMSA was lower in the amiodarone group [8.2 (5.8–13.5) mV·Hz vs. 9.6 (5.6–11.6), p = 0.042]. AMSA was a predictor of shock success in both groups but the predictive power was lower in the amiodarone group [Area Under the Curve (AUC) non-amiodarone group 0.812, 95%CI: 0.78–0.841 vs. AUC amiodarone group 0.706, 95%CI: 0.68–0.73; p < 0.001].

Conclusions

Amiodarone administration was independently associated with the probability of recording lower values of AMSA. In patients who have received amiodarone during cardiac arrest the predictive value of AMSA for shock success is significantly lower, but still statistically significant.
Study into impacts of formation sands on foam performance during drainage gas production—Take Qinghai Oilfield’s Sebei Gasfield for example
Authors: Li Nan , Jiang Weidong , Jiang Yixin , Yang Jinmin , Cao Guangqiang , Zheng Rui , Feng Daqiang , Wang Haoyu , Xu Zhiyu , Peng Bin , Li Furong , Kang Ruixin , Wu Qiubo
Published: May 2023

Foam drainage gas production is a gas production technology for low-pressure gas well in domestic and abroad industries. In Qinghai Oilfield’s Sebei Gasfield, sand existence often occurs during drainage production. In order to reveal the impacts of formation sands on drainage gas production, a lab test was conducted to make a study on foam performances of different foam drainage agents in case of sand existence. A laboratory test was employed to study the foam performances of different foam drainage agents under varies scenes of sand existence, to reveal the impacts of formation sands during foam drainage gas recovery. In this study, four foam drainage agents, i.e., OP-10, Sodium dodecyl benzene sulfonate, Hexadecyl Trimethyl Ammonium Bromide (CTAB), and Lauramidopropyl Hydroxy Sulfobetaine, were selected to compare with the field compound foam drainage agent QH-X1. Following patterns can be revealed by macroscopic and microscopic experimental results: Foam formed by an ionic-type foam drainage agent is more stable when the hydrophilic sand particles are smaller (which means they have larger specific surface areas and the adsorption energy levels between the sand and bubbles are higher); while foam formed by non-ionic foam drainage agent OP-10 has a short stable period because its polarity is weaker than that of ionic-type agent foam and the adsorbability of the foam on the hydrophilic particles is weak; under different sand conditions, the impacts of different foam drainage agent types on the average bubble area, bubble wall thickness, and Plateau Boundary are markedly different, and appear decreasing tendency with anionic foam drainage agents, is the trend can be more obvious when the sand particles are coarser. The study on QH-X1 shows that the foam parameters are generally lower as the sand particles are smaller, but the changing trends can be different after sand is involved. Particles constitute an adsorption film at the gas-liquid interface and a laminar structure within each interbubble thin liquid film, thus improving bubble coalescence and unloading stability. So, the inflow of the hydrophilic sands increases the stiffness, half-life period, and comprehensive rate of the QH-X1-induced foam.
Effects of dulaglutide combined with insulin degludec on glucose fluctuations and appetite in type 2 diabetes
Authors: Huang Jinxin , Hua Fei , Jiang Xiaohong , Zhang Xingguang , Yang Minxing , Wang Long , Huang Xiaolin , Luo Kaiming
Published: May 2023

Introduction

The aim of this study was to describe appetite and glucose fluctuation in type 2 diabetes mellitus patients initiating treatment with dulaglutide combined with insulin degludec.

Methods

This retrospective study of patients identified adults starting treatment with once-weekly (QW) dulaglutide combined with insulin degludec (experimental group) or insulin degludec alone (control group). Patients were followed for up to 6 months from treatment initiation. The clinical characteristics of patients, treatment patterns, CGM data, and appetite scores were obtained for the two groups.

Results

A total of 236 patients were included in this study. SDBG, MAGE, LAGE, and PPGE of the experimental group were lower than the control group’s (P < 0.05). The proportions of patients achieving a time in range (TIR) of ≥70% in the experimental group were higher than in the control group, with 43% and 10% on the second day, 88% and 47% on the fourth day, 95% and 47% on the seventh day, and 100% and 67% on the tenth day, respectively. Significant associations existed between TIR and the prevalence of islet function. At six months, 89.2% of patients in the experimental group were still using dulaglutide. Appetite decreased significantly at 1 week and increased at 3 months after treatment with dulaglutide.

Conclusion

Dulaglutide combined with insulin degludec significantly reduces glucose fluctuations in patients with type 2 diabetes mellitus and improves the TIR rate. However, the treatment on appetite could decrease in the first three months.
Response of leaf and soil C, N and P stoichiometry in different Pinus massoniana forest types to slope aspect in the Dabie mountains region of North subtropical, China
Authors: Qin Juan , Liu Ye , Bi Qingkang , Chen Ziqi , Zhang Bowen
Published: May 2023

Changes in slope aspect have important effects on the C, N and P nutrient contents and stoichiometric characteristics of plants and soils. As an important forest type in the subtropical region,Pinus massonianaforests play an important role in the restoration and development of forest ecosystems. In this study, the effect of slope aspect on the C, N and P contents and stoichiometry of leaves and soils of fourP. massonianaforest types (i.e., pureP. massonianaforest (PF),P. massoniana-Liquidambar formosanamixed forest (PLM),P. massoniana-Platycarya strobilaceamixed forest (PPM), andP. massoniana-Quercus acutissimamixed forest (PQM)) in north subtropical region of China were studied. The results showed that slope aspect had significant effects on soil C, N and P contents, leaf N content (LNC), soil C:P and N:P ratios, and leaf C:N and N:P ratios in fourP. massonianaforests. SOC, STN and STP contents differed significantly on the two slopes, with the three mixed forests having higher contents on the sunny slope than on the shady slope. Among the four forest types, the SOC, STN and STP contents of PQM were the highest on both slopes, and their soil C:P and N:P ratios were all the highest on the sunny slope. The LNC and leaf N:P ratios were higher on shady slope than on sunny slope, while the leaf C:N ratio was higher on sunny slope than on shady slope. At the species level, leaf N content and N:P ratio in the three deciduous species were higher than in the evergreen species ofP. massoniana, while leaf C content and C:N ratio were lower than inP. massoniana. The interaction between slope aspect and forest type caused a certain range of fluctuations in stoichiometry of the four forest types, but the overall ecological stoichiometry remained stable, and plant growth of the four forest types in the study area was limited by N. Slope-induced changes in soil nutrient content of the fourP. massonianaforest types directly affect leaf C:N:P stoichiometry, and soil N and P contents are key factors affecting leaf stoichiometry, especially soil P content.
Local adaptation, phenotypic plasticity, and species coexistence
Authors: Fontanari José F. , Matos Margarida , Santos Mauro
Published: May 2023

Understanding the mechanisms of species coexistence has always been a fundamental topic in ecology. Classical theory predicts that interspecific competition may select for traits that stabilize niche differences, although recent work shows that this is not strictly necessary. Here, we ask whether adaptive phenotypic plasticity could allow species coexistence (i.e., some stability at an equilibrium point) without ecological differentiation in habitat use. We used individual-based stochastic simulations defining a landscape composed of spatially uncorrelated or autocorrelated environmental patches, where two species with the same competitive strategies, not able to coexist without some form of phenotypic plasticity, expanded their ranges in the absence of a competition—colonization trade-off (a well-studied mechanism for species diversity). Each patch is characterized by a random environmental value that determines the optimal phenotype of its occupants. In such a scenario, only local adaptation and gene flow (migration) may interact to promote genetic variation and coexistence in the metapopulation. Results show that a competitively inferior species with adaptive phenotypic plasticity can coexist in a same patch with a competitively superior, non-plastic species, provided the migration rates and variances of the patches' environmental values are sufficiently large.
Research on an evaluation index system of critical emergency management capability based on machine learning in a complex scientific environment
Authors: Zhao Xianli , Hu Zhenlong
Published: May 2023

Introduction

A complex scientific environment requires multiple considerations for handling critical and emergency conditions with an addressing solution. Indexing and prioritizing are standard methods that are used in such settings to improve itinerary solutions. Significance of an indexing system relies on the benchmark solution and the strategy it implies.

Methods

The present study introduces an indexing strategy evaluation method (ISEM) to validate the efficiency of indexing systems. The proposed method identifies the root implication and the strategy parameters to address complex problems. The environmental and problem-specific parameters are determined to estimate the system's initial response. The capability through solution response, lag, and failure analysis is identified post the estimation through linear regression learning. The indexing system's operations are designed through linear itineraries to prevent interrupting failures. In addition, the environmental features are identified as augmenting factors to prevent strategy pausing across multiple indices.

Results and discussion

The proposed method employs linear analysis through itinerary levels of index evaluation for optimal, lagging, and failed implications. It also helps to identify specific reasons for solution improvement or retention from previous operations.
Sex-specific variations in global DNA methylation levels with age: a population-based exploratory study from North India
Authors: Kaushik Anshika , Chaudhary Vineet , Longkumer Imnameren , Saraswathy Kallur Nava , Jain Sonal
Published: May 2023

Purpose: Aging is one of the most important risk factors for a number of human diseases. Epigenetic alterations, including changes in DNA methylation patterns, have been reported to be one of the hallmarks of aging. Being a malleable process, the role of site-specific DNA methylation in aging is being extensively investigated; however, much less attention has been given to alterations in global DNA methylation with aging at the population level. The present study aims to explore overall and sex-specific variations in global DNA methylation patterns with age.

Methods: A total of 1,127 adult individuals (792 females) aged 30–75 years belonging to Haryana, North India, were recruited. Socio-demographic data was collected using a pretested interview schedule. Global DNA methylation analysis, of peripheral blood leucocyte (PBL) DNA, was performed using the ELISA-based colorimetric technique.

Results: Though the overall correlation analysis revealed a weak inverse trend between global DNA methylation and age, the adjusted regression model showed no significant association between global DNA methylation and age. In age-stratified analysis, global DNA methylation levels were found to be fairly stable until 60 years of age, followed by a decline in the above-60 age group. Further, no significant difference in DNA patterns methylation pattern was observed between males and females.

Conclusion: Overall, the study suggests a lack of association between global DNA methylation and age, especially until 60 years of age, and a similar DNA methylation pattern between males and females with respect to age.
Genetic analysis and QTL mapping of domestication-related traits in chili pepper (Capsicum annuum L.)
Authors: Lopez-Moreno Hector , Basurto-Garduño Ana Celia , Torres-Meraz Maria Alejandra , Diaz-Valenzuela Eric , Arellano-Arciniega Sergio , Zalapa Juan , Sawers Ruairidh J. H. , Cibrián-Jaramillo Angelica , Diaz-Garcia Luis
Published: May 2023

Chili pepper (Capsicum annuumL.) is one of the oldest and most phenotypically diverse pre-Columbian crops of the Americas. Despite the abundance of genetic resources, the use of wild germplasm and landraces in chili pepper breeding is limited. A better understanding of the evolutionary history in chili peppers, particularly in the context of traits of agronomic interest, can contribute to future improvement and conservation of genetic resources. In this study, an F2:3mapping population derived from a cross between aC. annuumwild accession (Chiltepin) and a cultivated variety (Puya) was used to identify genomic regions associated with 19 domestication and agronomic traits. A genetic map was constructed consisting of 1023 single nucleotide polymorphism (SNP) markers clustered into 12 linkage groups and spanning a total of 1,263.87 cM. A reciprocal translocation that differentiates the domesticated genome from its wild ancestor and other related species was identified between chromosomes 1 and 8. Quantitative trait locus (QTL) analysis detected 20 marker-trait associations for 13 phenotypes, from which 14 corresponded to previously identified loci, and six were novel genomic regions related to previously unexplored domestication-syndrome traits, including form of unripe fruit, seedlessness, deciduous fruit, and growth habit. Our results revealed that the genetic architecture ofCapsicumdomestication is similar to other domesticated species with few loci with large effects, the presence of QTLs clusters in different genomic regions, and the predominance of domesticated recessive alleles. Our analysis indicates the domestication process in chili pepper has also had an effect on traits not directly related to the domestication syndrome. The information obtained in this study provides a more complete understanding of the genetic basis ofCapsicumdomestication that can potentially guide strategies for the exploitation of wild alleles.
The importance of biomarker development for monitoring type 1 diabetes progression rate and therapeutic responsiveness
Authors: Fyvie Maxwell J. , Gillespie Kathleen M.
Published: May 2023

Type 1 diabetes (T1D) is an autoimmune condition of children and adults in which immune cells target insulin-producing pancreatic β-cells for destruction. This results in a chronic inability to regulate blood glucose levels. The natural history of T1D is well-characterized in childhood. Evidence of two or more autoantibodies to the islet antigens insulin, GAD, IA-2 or ZnT8 in early childhood is associated with high risk of developing T1D in the future. Prediction of risk is less clear in adults and, overall, the factors controlling the progression rate from multiple islet autoantibody positivity to onset of symptoms are not fully understood. An anti-CD3 antibody, teplizumab, was recently shown to delay clinical progression to T1D in high-risk individuals including adults and older children. This represents an important proof of concept for those at risk of future T1D. Given their role in risk assessment, islet autoantibodies might appear to be the most obvious biomarkers to monitor efficacy. However, monitoring islet autoantibodies in clinical trials has shown only limited effects, although antibodies to the most recently identified autoantigen, tetraspanin-7, have not yet been studied in this context. Measurements of beta cell function remain fundamental to assessing efficacy and different models have been proposed, but improved biomarkers are required for both progression studies before onset of diabetes and in therapeutic monitoring. In this mini-review, we consider some established and emerging predictive and prognostic biomarkers, including markers of pancreatic function that could be integrated with metabolic markers to generate improved strategies to measure outcomes of therapeutic intervention.
Monoclonal antibodies for prophylaxis and therapy of respiratory syncytial virus, SARS-CoV-2, human immunodeficiency virus, rabies and bacterial infections: an update from the World Association of Infectious Diseases and Immunological Disorders and the Italian Society of Antinfective Therapy
Authors: Esposito Susanna , Amirthalingam Gayatri , Bassetti Matteo , Blasi Francesco , De Rosa Francesco Giuseppe , Halasa Natasha B. , Hung Ivan , Osterhaus Albert , Tan Tina , Torres Juan Pablo , Vena Antonio , Principi Nicola
Published: May 2023

Monoclonal antibodies (mABs) are safe and effective proteins produced in laboratory that may be used to target a single epitope of a highly conserved protein of a virus or a bacterial pathogen. For this purpose, the epitope is selected among those that play the major role as targets for prevention of infection or tissue damage. In this paper, characteristics of the most important mABs that have been licensed and used or are in advanced stages of development for use in prophylaxis and therapy of infectious diseases are discussed. We showed that a great number of mABs effective against virus or bacterial infections have been developed, although only in a small number of cases these are licensed for use in clinical practice and have reached the market. Although some examples of therapeutic efficacy have been shown, not unlike more traditional antiviral or antibacterial treatments, their efficacy is significantly greater in prophylaxis or early post-exposure treatment. Although in many cases the use of vaccines is more effective and cost-effective than that of mABs, for many infectious diseases no vaccines have yet been developed and licensed. Furthermore, in emergency situations, like in epidemics or pandemics, the availability of mABs can be an attractive adjunct to our armament to reduce the impact. Finally, the availability of mABs against bacteria can be an important alternative, when multidrug-resistant strains are involved.
Pathological pathway analysis in an experimental rheumatoid arthritis model and the tissue repair effect of acupuncture at ST36
Authors: Zhang Yue , Wang Hui , Gong Yi-Nan , Yang Fu-Ming , Wang Shen-Jun , Liu Yang-Yang , Gui Yong-Qing , Xie Fei , Xu Zhi-Fang , Guo Yi
Published: May 2023

Rheumatoid arthritis (RA) is an autoimmune disease that generally affects the joints. In the face of inflammation-induced cartilage and bone damage, RA treatment remains insufficient. While research evidence indicates that acupuncture can exert anti-inflammatory and analgesic effects, improve the joint function of RA patients, and delay the disease, data on whether it can promote RA repair are lacking. Findings from the present work demonstrated that both the antigen-induced arthritis (AIA) and collagen-induced arthritis (CIA) models can simulate joint swelling of RA. The AIA model was more stable than the CIA model, with a higher incidence of successful arthritis modeling. Moreover, the AIA mice model could simulate the signal molecules and related pathological processes of the autoimmune response in RA, as well as major pathways related to RA and antigen immune response mechanisms. Manual acupuncture (MA) at Zusanli (ST36) significantly improved paw redness and swelling, pain, and inflammatory cell infiltration in the joints in AIA mice. The therapeutic effect of MA on AIA is achieved primarily through the regulation of steroid hormone biosynthesis, cell metabolism, and tissue repair processes. MA at ST36 can increase the gene contents of tissue repair growth factors, including PEG3, GADD45A, GDF5, FGF5, SOX2, and ATP6V1C2 in the inflammatory side joints of AIA mice, as well as the gene expression of the anti-inflammatory cytokine IL-10. In conclusion, acupuncture may alleviate RA in the joints via modulating the tissue healing process.
Arterial wall fibrosis in Takayasu arteritis and its potential for therapeutic modulation
Authors: Misra Durga Prasanna , Singh Kritika , Sharma Aman , Agarwal Vikas
Published: May 2023

Arterial wall damage in Takayasu arteritis (TAK) can progress despite immunosuppressive therapy. Vascular fibrosis is more prominent in TAK than in giant cell arteritis (GCA). The inflamed arterial wall in TAK is infiltrated by M1 macrophages [which secrete interleukin-6 (IL-6)], which transition to M2 macrophages once the inflammation settles. M2 macrophages secrete transforming growth factor beta (TGF-β) and glycoprotein non-metastatic melanoma protein B (GPNMB), both of which can activate fibroblasts in the arterial wall adventitia. Mast cells in the arterial wall of TAK also activate resting adventitial fibroblasts. Th17 lymphocytes play a role in both TAK and GCA. Sub-populations of Th17 lymphocytes, Th17.1 lymphocytes [which secrete interferon gamma (IFN-γ) in addition to interleukin-17 (IL-17)] and programmed cell death 1 (PD1)-expressing Th17 (which secrete TGF-β), have been described in TAK but not in GCA. IL-6 and IL-17 also drive fibroblast activation in the arterial wall. The Th17 and Th1 lymphocytes in TAK demonstrate an activation of mammalian target organ of rapamycin 1 (mTORC1) driven by Notch-1 upregulation. A recent study reported that the enhanced liver fibrosis score (derived from serum hyaluronic acid, tissue inhibitor of metalloproteinase 1, and pro-collagen III amino-terminal pro-peptide) had a moderate-to-strong correlation with clinically assessed and angiographically assessed vascular damage. In vitro experiments suggest the potential to target arterial wall fibrosis in TAK with leflunomide, tofacitinib, baricitinib, or mTORC1 inhibitors. Since arterial wall inflammation is followed by fibrosis, a strategy of combining immunosuppressive agents with drugs that have an antifibrotic effect merits exploration in future clinical trials of TAK.
Single-cell sequencing technology in colorectal cancer: a new technology to disclose the tumor heterogeneity and target precise treatment
Authors: Wen Rongbo , Zhou Leqi , Peng Zhiying , Fan Hao , Zhang Tianshuai , Jia Hang , Gao Xianhua , Hao Liqiang , Lou Zheng , Cao Fuao , Yu Guanyu , Zhang Wei
Published: May 2023

Colorectal Cancer (CRC) is one of the most common gastrointestinal tumors, and its high tumor heterogeneity makes traditional sequencing methods incapable of obtaining information about the heterogeneity of individual cancer cells in CRC. Therefore, single-cell sequencing technology can be applied to better analyze the differences in genetic and protein information between cells, to obtain genomic sequence information of single cells, and to more thoroughly analyze the cellular characteristics and interactions in the CRC microenvironment. This will provide a more comprehensive understanding of colorectal cancer development and metastasis and indicate the treatment plan and prognosis. In this study, we review the application of single-cell sequencing to analyze the tumor microenvironment of CRC, explore the mechanisms involved in CRC metastasis and progression, and provide a reference for potential treatment options.
Determination of phytoplankton community structure and biomass with HPLC-CHEMTAX and microscopic methods during winter and summer in the Qinzhou Bay of the Beibu Gulf
Authors: Pan Huizhu , Xu Mingben , Lan Caibi , Ma Jixian , Li Jie , Lu Jiachang , Lai Junxiang
Published: May 2023

The Qinzhou Bay, a typical semi-enclosed bay, is facing environmental pressure from local fast-growing industrial and aquacultural development. Dominant species of phytoplankton community (based on microscopic examination) show a trend of miniaturization, while pico-phytoplankton (based on CHEMTAX analysis) is widely distributed in Qinzhou Bay. However, most previous investigations of phytoplankton community based on microscopic method that undetected small-sized cell (< 3 μm), and limited by scarce studies on CHEMTAX analysis, the long-term dynamic data of small-size phytoplankton are lacking in Qinzhou Bay. It is recognized that combining microscopic examination with CHEMTAX analysis could provide a good taxonomic reliability for large cells and valuable information about small-size groups. In this study, microscopic examination and High Performance Liquid Chromatography (HPLC)-CHEMTAX analysis were employed to characterize the spatiotemporal variability of the phytoplankton community structure in Qinzhou Bay in winter and summer of 2021. The results of microscopic observations showed that the phytoplankton community was characterized by diatoms and dinoflagellates mainly. Diatoms dominated in both seasons, of which Skeletonema costatum bloom occurred in the summer. CHEMTAX analysis not only agreed well with microscopy data for diatoms and dinoflagellates, but also helped identification of other small-sized flagellates and cyanobacteria that hard to observe by microscope. The results of CHEMTAX analysis found that diatoms, prasinophytes and cryptophytes co-dominated the total chlorophyll a in winter while diatoms became the dominant group in summer. In addition, our results indicate that the proportion of small-sized flagellates has increased in the past decade in Qinzhou Bay, especially for cryptophytes. Temperature, nutrient availability, and selective grazing of oyster affected the succession of phytoplankton community from co-dominance of diatoms and flagellates in winter to absolute diatoms dominance in summer. The distribution of prasinophytes and cryptophytes on a spatial scale were related to the location of shellfish culture area and estuary, respectively, rather than by nutrients. Eutrophication, selective grazing of oyster and warming were the driving factors of long-term changes in phytoplankton composition in Qinzhou Bay. This study enhanced our understanding of entire phytoplankton community dynamics and its relationship with environmental factors in Qinzhou Bay.
International research in graphene-oxide based materials for net-zero energy, military and aeronautic applications catalysed by Tamaulipas, Mexico: a mini review
Authors: Caballero-Briones F. , Kaftelen-Odabaşı H. , Gnanaseelan N. , Ruiz-Perez F. , Tolentino-Hernandez R. V. , Kamaraj S. K. , Lopez Estrada S. M. , Espinosa-Faller F. J. , Jimenez-Melero E.
Published: May 2023

Graphene oxide, as a nanoscopic platform for functional materials, has been extensively studied for several applications. The present Mini Review stresses the collaborative research in graphene-oxide materials pivoted from the Group of Materials and Technologies for Energy, Health, and Environment at an Instituto Politecnico Nacional unit in Tamaulipas, in Northeastern Mexico, with Mexican, Turkish, and British collaborators. This review covers the recent works on photovoltaic and photocatalytic materials, coatings for thermonuclear reactors, and composites and metamaterials for military and aeronautic applications.
Clinical application of a modified predeposit autologous red blood cell apheresis in multistage spinal fusion: a single-center retrospective study
Authors: Xu Xiao-Ping , Que Wen-Jun , Yu Ze-Bo , Shen Jie-Liang , Hu Zhen-Ming , Yang Xiao-Liang , Hao Jie
Published: May 2023

Purpose

This study aimed to evaluate the efficacy and safety of predeposit autologous RBC apheresis (PARA) in patients undergoing multilevel spinal fusion surgery.

Methods

A total of 112 patients from January 2020 to June 2022 were divided into two groups according to PARA: the PARA group (n = 51) and the control group (n = 61). The baseline characteristics of the patients, outcomes, transfusion cost, hospitalization cost, length of stay, complications, and changes in hemoglobin and hematocrit levels between the two groups were compared.

Results

The baseline characteristics were similar in both groups. No significant differences were found in functional outcomes, including VAS score (p = 0.159), ODI score (p = 0.214), JOA score (p = 0.752), and SF-36 score (p = 0.188) between the PARA and control groups. The amount and rate of intraoperative and perioperative allogeneic RBC transfusion were significantly higher in the control group than in the PARA group (p < 0.001). The postoperative (9.04 ± 3.21 vs. 11.05 ± 3.84, p = 0.004) and total length of stay (15.78 ± 3.79 vs. 17.36 ± 4.08, p = 0.038) in the PARA group were significantly lower than those in the control group, respectively. Despite no difference in hospitalization cost (p = 0.737), the total blood transfusion cost in the PARA group was significantly lower, compared with the control group (p < 0.001). For safety evaluation, there were no significant differences in Hb and Hct levels between the two groups at admission, on postoperative day 1, and postoperative day 3, respectively (p > 0.05). Moreover, the number of postoperative infections in the PARA group was significantly lower than that in the control group (p = 0.038).

Conclusion

PARA was a novel, safe, and highly efficient technique for mass autologous blood preparation in a quite short preparation time. This method could significantly reduce the amount of allogeneic blood transfusion and length of stay, which could provide a theoretical basis for following clinical practice about the technique.
Egg yolk antibody combined with bismuth-based quadruple therapy in Helicobacter pylori infection rescue treatment: a single-center, randomized, controlled study
Authors: Cheng Sha , Li Huan , Luo Ju , Chi Jingshu , Zhao Wenfang , Lin Jiahui , Xu Canxia
Published: May 2023

Background

The increasing antibiotic resistance is the main issue causing Helicobacter pylori (H. pylori) eradication failure. As a nutritional supplement, Egg Yolk Antibody (Ig Y) provides a new approach for H. pylori infection rescue therapy.

Methods

In this randomized, controlled study, 100 H. pylori-positive patients with previous H. pylori eradication treatment were included. All individuals received standard bismuth-containing quadruple therapy twice daily (5 mg ilaprazole, 100 mg doxycycline, 500 mg clarithromycin or 1 g amoxicillin or 100 mg furazolidone, and 220 mg colloidal bismuth tartrate) for 14 days and were randomized to receive either twice daily 7 g Ig Y-H. pylori treatment (study group) or not (control group). 4 weeks after the end of treatment, urea breath tests were used to assess the H. pylori eradication rate. All participants scored by the Global Overall Symptom scale (GOS) and recorded adverse events during the trial.

Results

The H. pylori eradication rates were 84.0% (95% CI 73.5–94.5%) vs. 80.0% (95% CI 68.5–91.5%) in the study and control groups at intention-to-treat (ITT) analysis and 85.7% (95% CI 75.6–95.9%) vs. 80.0% (95% CI 68.5–91.5%) at per-protocol (PP) analysis, respectively. The number of over 80% symptom relief after treatment in the two groups was 27 (60%) and 12 (29.2%) (p < 0.05), and the incidences of adverse events were 4 (8%) and 6 (12%), respectively.

Conclusion

Both groups achieved satisfactory eradication efficiency in H. pylori rescue therapy and Ig Y-H. pylori effectively alleviates the symptoms with good compliance and fewer adverse effects.
Examining indicators of psychosocial risk and resilience in parents of autistic children
Authors: Dimachkie Nunnally Amanda , Factor Reina S. , Sturm Alexandra , Valluripalli Soorya Latha , Wainer Allison , Taylor Sandra , Ponzini Matthew , Abbeduto Leonard , Gulsrud Amanda C.
Published: May 2023

Background

Parents of autistic children experience increased levels of caregiver strain and adverse mental health outcomes, even in comparison to parents of children with other neurodevelopmental disabilities. Previous studies have largely attributed these increased levels of mental health concerns to their child behavioral concerns and autism symptomatology, but less attention has been given to other potential child factors, such as child adaptive functioning. Additionally, little is known about potential protective factors, such as parents’ emotion regulation (ER) abilities, that may ameliorate the experience of caregiver strain, anxiety, and depression.

Objective

The current study examined the impact of child characteristics (restricted and repetitive behaviors, adaptive functioning and behavioral concerns) on parent mental health outcomes (caregiver strain, anxiety, depression and wellbeing). Additionally, we explore parents’ ER abilities as a moderator of the impact of child characteristic on parents’ mental health outcomes.

Results

Results of linear mixed effect models indicated a significant relationship between parents’ ER abilities and all four parent outcomes. Additionally, children’s adaptive functioning abilities and repetitive behaviors (RRBs) were significant predictors of caregiving strain. Parents’ ER abilities were a significant moderator of the effect of children’s repetitive behaviors and adaptive functioning challenges on caregiver strain, such that better ER abilities mitigated the impact of child clinical factors on caregiver strain. Finally, a significant difference was detected for mothers’ and fathers’ mental health, with mothers reporting higher caregiver strain, and more symptoms of anxiety and depression than did fathers.

Conclusion

This study leveraged a large sample of autistic children and their biological parents to examine the relationship between children’s clinical characteristics and parents’ psychological wellbeing. Results indicate that, although parents of autistic children do experience high rates of internalizing mental health concerns that relate to child adaptive functioning and RRBs, parent ER abilities act as a protective factor against parents’ adverse mental health outcomes. Further, mothers in our sample reported significantly higher rates of depression, anxiety, and caregiver strain, as compared with fathers.
Source localization using recursively applied and projected MUSIC with flexible extent estimation
Authors: Hecker Lukas , Tebartz van Elst Ludger , Kornmeier Jürgen
Published: May 2023

Magneto- and electroencephalography (M/EEG) are widespread techniques to measure neural activity in-vivo at a high temporal resolution but low spatial resolution. Locating the neural sources underlying the M/EEG poses an inverse problem, which is ill-posed. We developed a new method based on Recursive Application of Multiple Signal Classification (MUSIC). Our proposed method is able to recover not only the locations but, in contrast to other inverse solutions, also the extent of active brain regions flexibly (FLEX-MUSIC). This is achieved by allowing it to search not only for single dipoles but also dipole clusters of increasing extent to find the best fit during each recursion. FLEX-MUSIC achieved the highest accuracy for both single dipole and extended sources compared to all other methods tested. Remarkably, FLEX-MUSIC was capable to accurately estimate the level of sparsity in the source space (r = 0.82), whereas all other approaches tested failed to do so (r ≤ 0.18). The average computation time of FLEX-MUSIC was considerably lower compared to a popular Bayesian approach and comparable to that of another recursive MUSIC approach and eLORETA. FLEX-MUSIC produces only few errors and was capable to reliably estimate the extent of sources. The accuracy and low computation time of FLEX-MUSIC renders it an improved technique to solve M/EEG inverse problems both in neuroscience research and potentially in pre-surgery diagnostic in epilepsy.
Nicotinic acetylcholine receptors and learning and memory deficits in Neuroinflammatory diseases
Authors: Echeverria Valentina , Mendoza Cristhian , Iarkov Alex
Published: May 2023

Animal survival depends on cognitive abilities such as learning and memory to adapt to environmental changes. Memory functions require an enhanced activity and connectivity of a particular arrangement of engram neurons, supported by the concerted action of neurons, glia, and vascular cells. The deterioration of the cholinergic system is a common occurrence in neurological conditions exacerbated by aging such as traumatic brain injury (TBI), posttraumatic stress disorder (PTSD), Alzheimer’s disease (AD), and Parkinson’s disease (PD). Cotinine is a cholinergic modulator with neuroprotective, antidepressant, anti-inflammatory, antioxidant, and memory-enhancing effects. Current evidence suggests Cotinine’s beneficial effects on cognition results from the positive modulation of the α7-nicotinic acetylcholine receptors (nAChRs) and the inhibition of the toll-like receptors (TLRs). The α7nAChR affects brain functions by modulating the function of neurons, glia, endothelial, immune, and dendritic cells and regulates inhibitory and excitatory neurotransmission throughout the GABA interneurons. In addition, Cotinine acting on the α7 nAChRs and TLR reduces neuroinflammation by inhibiting the release of pro-inflammatory cytokines by the immune cells. Also, α7nAChRs stimulate signaling pathways supporting structural, biochemical, electrochemical, and cellular changes in the Central nervous system during the cognitive processes, including Neurogenesis. Here, the mechanisms of memory formation as well as potential mechanisms of action of Cotinine on memory preservation in aging and neurological diseases are discussed.
Radiotherapy combined with immunotherapy successfully treated one case of anaplastic thyroid cancer: A case report
Authors: Xing Yurou , Wang Yongsheng , Wu Xin
Published: May 2023

Background

Anaplastic thyroid cancer (ATC) is a rare but highly fatal form of thyroid cancer. This highly malignant tumor progresses rapidly and is prone to relapse and metastasis, with a poor prognosis. Novel treatments have improved survival in recent years, but the outcome of treatment is not satisfactory.

Case presentation

We report a case of multiple postoperative recurrences of papillary thyroid carcinoma that later transformed into undifferentiated carcinoma. The patient’s neck mass was huge and the operation was unsuitable. Then, she achieved remarkable tumor shrinkage by tislelizumab immunotherapy combined with radiotherapy.

Conclusion

This case indicates that radiotherapy combined with immunotherapy is a promising treatment for ATC. Such a combined approach warrants further study.
MRI-based clinical radiomics nomogram may predict the early response after concurrent chemoradiotherapy in locally advanced nasopharyngeal carcinoma
Authors: Wu Mengxing , Xu Weilin , Fei Yinjiao , Li Yurong , Yuan Jinling , Qiu Lei , Zhang Yumeng , Chen Guanhua , Cheng Yu , Cao Yuandong , Sun Xinchen , Zhou Shu
Published: May 2023

Objective

Tumor residue after concurrent chemoradiotherapy (CCRT) in nasopharyngeal carcinoma (NPC) patients often predicts poor prognosis. Thus, the objective of this retrospective study is to develop a nomogram that combines magnetic resonance (MRI) radiomics features and clinical features to predict the early response of locally advanced nasopharyngeal carcinoma (LA-NPC).

Methods

A total of 91 patients with LA-NPC were included in this study. Patients were randomly divided into training and validation cohorts at a ratio of 3:1. Univariate and multivariate analyses were performed on the clinical parameters of the patients to select clinical features to build a clinical model. In the training cohort, the Least Absolute Shrinkage and Selection Operator (LASSO) regression model was used to select radiomics features for construction of a radiomics model. The logistic regression algorithm was then used to combine the clinical features with the radiomics features to construct the clinical radiomics nomogram. Receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA) were drawn to compare and verify the predictive performances of the clinical model, radiomics model, and clinical radiomics nomogram.

Results

Platelet lymphocyte ratio (PLR) and nasopharyngeal tumor volume were identified as independent predictors of early response in patients with locally advanced nasopharyngeal carcinoma. A total of 5502 radiomics features were extracted, from which 25 radiomics features were selected to construct the radiomics model. The clinical radiomics nomogram demonstrated the highest AUC in both the training and validation cohorts (training cohort 0.975 vs 0.973 vs 0.713; validation cohort 0.968 vs 0.952 vs 0.706). The calibration curve and DCA indicated good predictive performance for the nomogram.

Conclusion

A clinical radiomics nomogram, which combines clinical features with radiomics features based on MRI, can predict early tumor regression in patients with LA-NPC. The performance of the nomogram is superior to that of either the clinical model or radiomics model alone. Therefore, it can be used to identify patients without CR at an early stage and provide guidance for personalized therapy.