bluuebunny/crossref_metadata_2025 huggingface dataset:
A Home-Based Individual Cognitive Stimulation Program for Older Adults With Cognitive Impairment: A Randomized Controlled Trial
Authors: Silva Rosa , Bobrowicz-Campos Elzbieta , Santos-Costa Paulo , Cruz Ana Rita , Apóstolo João
Published: November 2021

Objective: This study aims to assess the feasibility and meaningfulness of a home-based individual cognitive stimulation (iCS) program delivered by caregivers to persons with cognitive impairment (PwCIs). It also aims to assess whether the older adults receiving this program improved their cognitive, neuropsychiatric, and depressive symptoms and quality of life and whether their caregivers improved their mental and physical health.

Methods: A randomized controlled trial (RCT) was conducted with PwCI-caregiver dyads recruited from the community. Participants were allocated to two groups: intervention (n = 28) and control (n = 24). The intervention group received the European Portuguese version of the Individual Cognitive Stimulation Program—Making a Difference 3 (MD3-P). The control group received usual care. The iCS therapy program was implemented three times a week for 12 weeks. Caregivers were supported by the researchers to deliver the sessions at home. Participants were assessed at baseline and at the end of the intervention (week 13). Feasibility and meaningfulness were assessed through the attrition rate, adherence, and degree of satisfaction with the sessions. Four interviews were conducted (after week 13) to understand participants’ experiences.

Results: The attrition rate was 23.1%. The dyads reported that they did not have high expectations about the iCS program before starting the study. Nevertheless, as the program evolved, caregivers noted that their family members had improved some areas of functioning. Intention-to-treat analysis based on group differences revealed a significant improvement in PwCIs’ cognition, specifically in their orientation and ability to follow commands. The intervention had no impact on other variables such as caregivers’ physical and mental health.

Conclusion: The iCS program implemented by caregivers showed promising results in improving PwCIs’ cognition. The participants who completed the intervention attributed a positive meaning to the MD3-P, confirming it as a valid non-pharmacological therapeutic approach to reducing frailty in PwCIs in community settings.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier [NCT03514095].
Resilience Programs for Children and Adolescents: A Systematic Review and Meta-Analysis
Authors: Pinto Tatiana Matheus , Laurence Paulo Guirro , Macedo Cristiane Rufino , Macedo Elizeu Coutinho
Published: November 2021

Resilience may be defined as the ability to recover and adapt to adverse situations. Given that resilience involves cognitive and behavioral aspects, it could be promoted based on strategies that favor them, especially during childhood and adolescence. As a result, several resilience-focused programs have been developed and studied. This systematic review of Randomized Controlled Trials (RCTs) aimed to assess resilience-focused programs for children (<12 years old) and adolescents (12–22 years old) compared to active (treatment as usual, other program modalities, and educational curriculum at school) or inactive (waiting list, no treatment) control groups. We performed a systematic review of meta-analyses of RCTs. The following databases were searched: Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, Embase, and PsycINFO. Two authors independently selected the studies, extracted the data, and assessed the studies’ risk of bias. Meta-analyses of random effects were conducted to calculate the standard mean differences (SMD) and 95% confidence interval (CI) of program effectiveness. Of the 17 RCTs that met the inclusion criteria, 13 provided sufficient data to assess the effectiveness of the programs after their implementation. Meta-analyses indicated overall effectiveness of the programs in promoting resilience (SMD = 0.48, 95% CI [0.15, 0.81], p = 0.0077). The subgroup analysis indicated effectiveness only among adolescents’ resilience (SMD = 0.48, 95% CI [0.08, 0.88], p = 0.02). The follow-up analysis also indicated evidence of continuation of results within a period of up to 6 months up (SMD = 0.12, 95% CI [−0.44, 0.69], p = 0.02). These results indicated the effectiveness of promoting resilience, especially in adolescents, and its continuation in follow-up analyses. These findings are promising in the field of resilience programs; however, further studies are necessary to analyze the different possible characteristics of programs and their results.

Clinical Trial Registration: [https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020179874], [CRD42020179874].
The Saliency of Snake Scales and Leopard Rosettes to Infants: Its Relevance to Graphical Patterns Portrayed in Prehistoric Art
Authors: Coss Richard G. , Charles Eric P.
Published: November 2021

Geometrically arranged spots and crosshatched incised lines are frequently portrayed in prehistoric cave and mobiliary art. Two experiments examined the saliency of snake scales and leopard rosettes to infants that are perceptually analogous to these patterns. Experiment 1 examined the investigative behavior of 23 infants at three daycare facilities. Four plastic jars (15×14.5cm) with snake scales, leopard rosettes, geometric plaid, and plain patterns printed on yellowish-orange paper inside were placed individually on the floor on separate days during playtime. Fourteen 7–15-month-old infants approached each jar hesitantly and poked it before handling it for five times, the criterion selected for statistical analyses of poking frequency. The jars with snake scales and leopard rosettes yielded reliably higher poking frequencies than the geometric plaid and plain jars. The second experiment examined the gaze and grasping behavior of 15 infants (spanning 5months of age) seated on the laps of their mothers in front of a table. For paired comparisons, the experimenter pushed two of four upright plastic cylinders (13.5×5.5cm) with virtually the same colored patterns simultaneously toward each infant for 6s. Video recordings indicated that infants gazed significantly longer at the cylinders with snake scales and leopard rosettes than the geometric plaid and plain cylinders prior to grasping them. Logistic regression of gaze duration predicting cylinder choice for grasping indicated that seven of 24 paired comparisons were not significant, all of which involved choices of cylinders with snake scales and leopard rosettes that diverted attention before reaching. Evidence that these biological patterns are salient to infants during an early period of brain development might characterize the integration of subcortical and neocortical visual processes known to be involved in snake recognition. In older individuals, memorable encounters with snakes and leopards coupled with the saliency of snake scales and leopard rosettes possibly biased artistic renditions of similar patterns during prehistoric times.
Gaining a Better Understanding of the Types of Organizational Culture to Manage Suffering at Work
Authors: Assens-Serra Jordi , Boada-Cuerva Maria , Serrano-Fernández María-José , Agulló-Tomás Esteban
Published: November 2021

Organizational culture is a central concept in research due to its importance in organizational functioning and suffering of employees. To better manage suffering, it is necessary to better understand the intrinsic characteristics of each type of culture and also its relationships with the environment. In this study, we used the multiple regression analysis to analyze the capacity of eight environment variables, five business strategies, and eight organizational competencies to predict the presence of Clan, Market, and Hierarchy cultures (Cameron and Quinn, 1999) in a subsample of Spanish managers (n1 = 362) and a subsample of Peruvian managers (n2 = 1,317). Contrary to what most of the literature suggests, we found almost no relationship between the environmental variables and the culture types. Strategy and competencies, in contrast, do have a significant predictive capacity, showing 9 links with the Clan culture, 7 with the Hierarchy culture, and 10 with the Market culture. In conclusion, this study has found the important characteristics of the types of organizational culture that could be useful to better manage the suffering of employees.
Cellular Bioenergetics and AMPK and TORC1 Signalling in Blood Lymphoblasts Are Biomarkers of Clinical Status in FMR1 Premutation Carriers
Authors: Loesch Danuta Z. , Kemp Bruce E. , Bui Minh Q. , Fisher Paul R. , Allan Claire Y. , Sanislav Oana , Ngoei Kevin R. W. , Atkinson Anna , Tassone Flora , Annesley Sarah J. , Storey Elsdon
Published: November 2021

Fragile X Associated Tremor/Ataxia Syndrome (FXTAS) is a neurodegenerative disorder affecting carriers of premutation alleles (PM) of the X-linked FMR1 gene, which contain CGG repeat expansions of 55–200 range in a non-coding region. This late-onset disorder is characterised by the presence of tremor/ataxia and cognitive decline, associated with the white matter lesions throughout the brain, especially involving the middle cerebellar peduncles. Nearly half of older male and ~ 20% of female PM carriers develop FXTAS. While there is evidence for mitochondrial dysfunction in neural and some peripheral tissues from FXTAS patients (though less obvious in the non-FXTAS PM carriers), the results from peripheral blood mononuclear cells (PBMC) are still controversial. Motor, cognitive, and neuropsychiatric impairments were correlated with measures of mitochondrial and non-mitochondrial respiratory activity, AMPK, and TORC1 cellular stress-sensing protein kinases, and CGG repeat size, in a sample of adult FXTAS male and female carriers. Moreover, the levels of these cellular measures, all derived from Epstein- Barr virus (EBV)- transformed and easily accessible blood lymphoblasts, were compared between the FXTAS (N= 23) and non-FXTAS (n= 30) subgroups, and with baseline data from 33 healthy non-carriers. A significant hyperactivity of cellular bioenergetics components as compared with the baseline data, more marked in the non-FXTAS PMs, was negatively correlated with repeat numbers at the lower end of the CGG-PM distribution. Significant associations of these components with motor impairment measures, including tremor-ataxia and parkinsonism, and neuropsychiatric changes, were prevalent in the FXTAS subgroup. Moreover, a striking elevation of AMPK activity, and a decrease in TORC1 levels, especially in the non-FXTAS carriers, were related to the size of CGG expansion. The bioenergetics changes in blood lymphoblasts are biomarkers of the clinical status of FMR1 carriers. The relationship between these changes and neurological involvement in the affected carriers suggests that brain bioenergetic alterations are reflected in this peripheral tissue. A possible neuroprotective role of stress sensing kinase, AMPK, in PM carriers, should be addressed in future longitudinal studies. A decreased level of TORC1—the mechanistic target of the rapamycin complex, suggests a possible future approach to therapy in FXTAS.
Neurocognition and Social Cognition— Possibilities for Diagnosis and Treatment in Ultra-High Risk for Psychosis State
Authors: Rek-Owodziń Katarzyna , Tyburski Ernest , Waszczuk Katarzyna , Samochowiec Jerzy , Mak Monika
Published: November 2021

In recent decades, clinicians have developed the construct of ultra-high risk (UHR) for psychosis to characterize the prodromal phase of psychosis or classify people with weakly expressed psychotic symptoms. In this conceptual analysis, we have gathered up-to-date data about the clinical picture of neurocognition and social cognition in people at UHR for psychosis. We also discuss treatment options. A well-chosen therapeutic approach can help to deal with difficulties and delay or even prevent the development of full-blown psychotic disorders in the UHR group. Despite much evidence supporting the benefits of therapy, early interventions are still not as widely used as they should be. Thus, a better understanding of the UHR state is very important for all healthcare workers.
Occurrence of SARS-CoV-2 in Indoor Environments With Increased Circulation and Gathering of People
Authors: Dziedzinska Radka , Kralik Petr , Šerý Omar
Published: November 2021

At the time of sampling (2020/2021), the number of new cases of SARS-CoV-2-positive individuals in the Czech Republic significantly exceeded the numbers in neighboring countries and in the EU. In terms of the number of deaths, the country ranked near the top of the list. Legislative orders required wearing masks indoors, disinfecting surfaces in public places, and limiting the number of people per sales area in commercial spaces. Due to an situation, most schools and shops were closed. The entire country anticipated a total lockdown. To assess the risk to public health regarding SARS-CoV-2 transmission, air and surfaces were sampled in two public places: a post office and a shopping center. Samples were also collected at the COVID-19 unit at the local hospital. Neither air nor surface samples were positive for SARS-CoV-2 virus particles in the post office or shopping center. Positive results were found in the hospital ward, with floors being the most and highest contaminated surface. Based on our results, we believe that public places do not pose a risk in relation to SARS-CoV-2 transmission, especially when epidemiological measures to reduce transmission are followed, such as wearing masks, using disinfectant or limiting the number of customers per retail establishment.
Integrated Pest Management Protocols for Space-Based Bioregenerative Life Support Systems
Authors: Schuerger Andrew C.
Published: November 2021

Human missions to the Moon and Mars will necessarily increase in both duration and complexity over the coming decades. In the past, short-term missions to low-Earth orbit (LEO) or the Moon (e.g., Apollo) utilized physiochemical life support systems for the crews. However, as the spatial and temporal durations of crewed missions to other planetary bodies increase, physiochemical life support systems become burdened with the requirement of frequent resupply missions. Bioregenerative life support systems (BLSS) have been proposed to replace much of the resupply required of physiochemical systems with modules that can regenerate water, oxygen, and food stocks with plant-based biological production systems. In order to protect the stability and productivity of BLSS modules (i.e., small scale units) or habitats (i.e., large scale systems), an integrated pest management (IPM) program is required to prevent, mitigate, and eliminate both insect pests and disease outbreaks in space-based plant-growing systems. A first-order BLSS IPM program is outlined herein that summarizes a collection of protocols that are similar to those used in field, greenhouse, and vertical-farming agricultural systems. However, the space environment offers numerous unusual stresses to plants, and thus, unique space-based IPM protocols will have to be developed. In general, successful operation of space-based BLSS units will be guided by IPM protocols that (1) should be established early in the mission design phase to be effective, (2) will be dynamic in nature changing both spatially and temporally depending on the successional processes afoot within the crewed spacecraft, plant-growing systems, and through time; and (3) can prevent insect/phytopathology outbreaks at very high levels that can approach 100% if properly implemented.
Prospects for Abiotic Stress Tolerance in Crops Utilizing Phyto- and Bio-Stimulants
Authors: Rai Nidhi , Rai Shashi Pandey , Sarma Birinchi Kumar
Published: November 2021

Environmental stressors such as salinity, drought, high temperature, high rainfall, etc. have already demonstrated the negative impacts on plant growth and development and thereby limiting productivity of the crops. Therefore, in the time to come, more sustainable efforts are required in agricultural practices to ensure food production and security under such adverse environmental conditions. A most promising and eco-friendly way to achieve this goal would be to apply biostimulants to address the environmental concerns. Non-microbial biostimulants such as humic substances (HA), protein hydrolysate, plant-based products and seaweed extracts (SWE), etc. and/or microbial inoculants comprising of plant growth-promoting microbes such as arbuscular mycorrhizal fungi (AMF), fluorescent and non-fluorescentPseudomonas, Trichodermaspp.,Bacillusspp. etc. have tremendous potentiality to enhance plant growth, flowering, crop productivity, nutrient use efficiency (NUE) and translocation, as well as enhancing tolerance to a wide range of abiotic stresses by modifying physiological, biological and biochemical processes of the crop-plants. Similarly, application techniques and timing are also important to achieve the desired results. In this article we discussed the prospects of using seaweed, microbial, and plant-based biostimulants either individually or in combination for managing environmental stresses to achieve food security in a sustainable way. Particular attention was given to the modifications that take place in plant's physiology under adverse environmental conditions and how different biostimulants re-program the host's physiology to withstand such stresses. Additionally, we also discussed how application of biostimulants can overcome the issue of nutrient deficiency in agricultural lands and improve their use efficiency by crop plants.
Does Information Acquisition Influence the Adoption of Sustainable Land Management Practices? Evidence From Mpumalanga Province South Africa
Authors: Oduniyi Oluwaseun Samuel , Tekana Sibongile Sylvia
Published: November 2021

Agricultural information plays a vital role in adopting agricultural technology. The study explored if information acquisition is related to the adoption of sustainable land management practices (SLMP) and jointly decided in Mpumalanga Province of South Africa. Primary data were collected through face-to-face interviews, using a proportionate random sampling technique to get 250 smallholder farmers to participate in the survey. A seemingly unrelated bivariate probit (SUBP) model and a recursive bivariate probit (RBP) model were adopted to examine the objective. The statistical estimation of the SUBP showed that there is a relationship, an empirical association between information acquisition and SLMP; while RBP estimation showed that information acquisition was exogenous in the adoption model; thus, the decision to acquire information and adopt SLMP was not jointly decided. Therefore, the study presents the determinants of information acquisition alongside with the adoption of SLPM. The result from the SUBP model, indicated that the years spent in school; agricultural extension service; the number of extension visits and the years of farming, influenced both information acquisition and the adoption of SLMP. The cost attached positively influenced the adoption of SLMP; while gender, marital status and age only influenced the information acquisition.
Sleeping Beauty: Anesthesia May Promote Relapse in Dogs With Diffuse Large B-Cell Lymphoma in Complete Remission After Chemo-Immunotherapy
Authors: Faroni Eugenio , Sabattini Silvia , Lenzi Jacopo , Guerra Dina , Comazzi Stefano , Aresu Luca , Mazzanti Alessia , Zanardi Stefano , Cola Veronica , Lotito Emilio , Marconato Laura
Published: November 2021

Surgery-induced stress and anesthesia-related immunosuppression are believed to play a critical role in human oncology patients. Studies have hypothesized that anesthesia influences patients' outcome, promoting tumor recurrence and metastasis. Aim of the study was to investigate whether anesthesia promoted relapse in dogs with diffuse large B-cell lymphoma (DLBCL). Medical records were searched for dogs with DLBCL, that were in complete remission (CR) after the same chemo-immunotherapy protocol. Dogs receiving anesthesia were included if the procedure was performed while in CR. Time to relapse (TTR) was obtained via Kaplan–Meier method. Association between anesthesia and relapse was assessed using a nested case-control design and estimated using conditional logistic regression. Sixty-one dogs with DLBCL were included. Overall median TTR was 329 days (95% CI, 281–377). Forty-eight (79%) dogs relapsed during the study period, while 13 (21%) were still in CR at data analysis closure. Eighteen (30%) dogs received anesthesia with opioids, propofol, and isoflurane or sevoflurane. The relative risk of lymphoma relapse for dogs undergoing anesthesia was significantly higher compared with dogs not undergoing anesthesia, with an odds ratio of 3.09 (P = 0.019) on multivariable analysis. Anesthesia may promote relapse in dogs with DLBCL treated with chemo-immunotherapy, although a role of perioperative stress cannot be ultimately excluded. Considering the high frequency of anesthetic procedures required for diagnostic and therapeutic protocols among oncology patients, it is of utmost interest to characterize the effects of single anesthetic agents on the immune system. Further prospective studies are needed to better define the impact of anesthesia on patients' outcome.
Research on the Spatial Network Structure and Influencing Factors of the Allocation Efficiency of Agricultural Science and Technology Resources in China
Authors: Wang Yameng , Chen Zhe , Wang Xiumei , Hou Mengyang , Wei Feng
Published: November 2021

The allocation efficiency of China’s agricultural science and technology resources (ASTR) varies in different regions and has a complicated spatial distribution pattern. To visually study whether there are correlations and mutual influences between the allocation efficiency of different regions, we use social network analysis methods (SNA). The study found that: (i) China’s allocation efficiency of ASTR has significant spatial correlation and spillover effects. The overall network density is declining. (ii) The spatial correlation network has significant regional heterogeneity. Some eastern provinces play “intermediaries” and “bridges” in the network. (iii) Geographical proximity, differences in economic development levels, industrial structure levels, and differences in urbanization have a significant impact on the formation of spatial association networks.
Regulation of Phosphorus Supply on Nodulation and Nitrogen Fixation in Soybean Plants with Dual-Root Systems
Authors: Li Hongyu , Wang Xiangxiang , Liang Quanxi , Lyu Xiaochen , Li Sha , Gong Zhenping , Dong Shoukun , Yan Chao , Ma Chunmei
Published: November 2021

Phosphorus (P) is an important nutrient affecting nodulation and nitrogen fixation in soybeans. To further investigate the relationship of phosphorus with soybean nodulation and nitrogen fixation, the seedling grafting technique was applied in this study to prepare dual-root soybean systems for a sand culture experiment. From the unfolded cotyledon stage to the initial flowering stage, one side of each dual-root soybean system was irrigated with nutrient solution containing 1 mg/L, 31 mg/L, or 61 mg/L of phosphorus (phosphorus-application side), and the other side was irrigated with a phosphorus-free nutrient solution (phosphorus-free side), to study the effect of local phosphorus supply on nodulation and nitrogen fixation in soybean. The results are described as follows: (1) Increasing the phosphorus supply increased the nodules weight, nitrogenase activity, ureide content, number of bacteroids, number of infected cells, and relative expression levels of nodule nitrogen fixation key genes (GmEXPB2, GmSPX5, nifH, nifD, nifK, GmALN1, GmACP1, GmUR5, GmPUR5, and GmHIUH5) in root nodules on the phosphorus-application side. Although the phosphorus-application and phosphorus-free sides demonstrated similar changing trends, the phosphorus-induced increases were more prominent on the phosphorus-application side, which indicated that phosphorus supply systematically regulates nodulation and nitrogen fixation in soybean. (2) When the level of phosphorus supply was increased from 1 mg/L to 31 mg/L, the increase on the P– side root was significant, and nodule phosphorus content increased by 57.14–85.71% and 68.75–75.00%, respectively; ARA and SNA were 218.64–383.33% and 11.41–16.11%, respectively, while ureide content was 118.18–156.44%. When the level of phosphorus supply was increased from 31mg/L to 61mg/L, the increase in the regulation ability of root and nodule phosphorus content, ARA, SNA, and ureide content were low for roots, and the value for nodules was lower than when the phosphorus level increased from 1 mg/L to 31 mg/L. (3) A high-concentration phosphorus supply on one side of a dual-root soybean plant significantly increased the phosphorus content in the aboveground tissues, as well as the roots and nodules on both sides. In the roots on the phosphorus-free side, the nodules were prioritized for receiving the phosphorus transported from the aboveground tissues to maintain their phosphorus content and functionality.
The Effects of Bioinoculants Based on Mycorrhizal and Trichoderma spp. Fungi in an Apple Tree Nursery under Replantation Conditions
Authors: Zydlik Zofia , Zydlik Piotr , Wieczorek Robert
Published: November 2021

Both mycorrhizal and Trichoderma spp. fungi are known for antagonistic effects against certain biological pathogens causing apple replant disease (ARD). The aim of this study was to assess the effectiveness of the bioinoculants based on endomycorrhizal and Trichoderma spp. fungi on the biological properties of soil as well as the parameters of the apple tree growths in a fruit tree nursery under replantation conditions. A two-year experiment was conducted on Jonagold apple trees grafted on to M.9 rootstock in western Poland. The trees were planted in the replant soil—from areas used for the production of apple trees, and in the crop rotation soil, that had not been used for nursery purposes before. A mycorrhizal inoculum and preparations containing Trichoderma spp. fungi were applied to the replant soil. Biological properties of the soil and the growth of the aerial and underground parts of the apple trees were assessed. The enzymatic (dehydrogenases and protease) and respiratory activity of the replant soil was significantly lower than that of the crop rotation soil. The apple trees grew worse when exposed to the ARD conditions. The effectiveness of applied bioinoculants in mitigating the effects of replantation in the nursery were shown. Both the treatment mycorrhization and the application of bioinoculants containing Trichoderma spp. increased the respiratory and enzymatic activity of the replant soil. The growth of the root system and the aerial parts of the trees (including leaves) was much better after the combined use of both types of fungi than in the replant soil that had not received the fungal treatment.
AS-Solar, a Tool for Predictive Maintenance of Solar Groundwater Pumping Systems
Authors: Cervera-Gascó Jorge , Montero Jesús , Moreno Miguel A.
Published: November 2021

Energy for water abstraction limits the viability of some irrigable areas. Increasing efficiency and introducing renewable energy can reduce energy cost. Solar pumping is a widely recognized renewable energy solution. These pumping systems suffer special wear out due to sudden changes and for having working conditions far from the nominal points. Thus, monitoring systems are becoming more frequent for maintenance issues. A new decision support system, named AS-Solar, was developed to perform predictive maintenance. This model permits detecting if the source of the anomaly in the pump performance is the pump, the electrical components (motor, variable frequency drive (VFD) or cables) or the pumping pipe. It demands real-time data from the monitoring system and an accurate simulation model, together with an optimization process that helps in the decision making in predictive maintenance. To validate the developed model, it was applied to a complex case study of a solar pumping system of 40 kWp that abstracts groundwater from nearly 200 m deep. This pumping system has a VFD, two lines of cables up to the pump and aggressive water with slimes, which causes different problems in the pumping system. In this case study, the AS-Solar model shows an acceptable accuracy, with a relative error (RE) of the 2.9% in simulated power and 7.9% in simulated discharge.
A Review: Soil Management, Sustainable Strategies and Approaches to Improve the Quality of Modern Viticulture
Authors: Cataldo Eleonora , Fucile Maddalena , Mattii Giovan Battista
Published: November 2021

Conservative and sustainable soil management in vineyards is an approach of primary importance not only for the yield (tons per hectare) and grapes’ quality (primary and secondary metabolites), but also for the greater preservation of the ecosystem. Compared to sustained-conventional tillage and perpetual applications of fertilizers and phytopharmaceutical, these techniques give a primary role for safeguarding biodiversity, conserving soil fertility, and keeping vegetative–productive balance. The soil and, consequently, the wine production are in fact an intimate ecosystem jeopardized not only by a reckless approach by man (technical input, such as pesticides, fuel, fertilizers, and herbicides, are estimated to be responsible for 24% of anthropogenic greenhouse gases emissions), but also by climate change, as rising summer temperatures and reduced precipitation leads to production declines and water shortages in the soil. In fact, there are several risks associated with unbalanced soil management, such as compaction, pollution, soil erosion, soil organic matter (SOM) depletion, and loss of biodiversity, that lead to a drop in grape quality and quantity. In this context, soil management in viticulture and sustainable strategies assume greater significance to improve the quality of modern viticulture. This review aims to highlight new agronomic techniques capable of enhancing the resilience of the system and contributing to conservation and ecosystem services provision, especially as wine consumers increasingly appreciate environmentally friendly farming practices. In particular, the review aims to focus the positive implications and repercussions as a result of these practices (e.g., compost, vermicompost, biochar, Ascophyllum nodosum, Arbuscular mycorrhizal fungi (AMF), Trichoderma, zeolite, partial root drying, cover cropping, and mulching).
Effects of Supplementation with a Quebracho Tannin Product as an Alternative to Antibiotics on Growth Performance, Diarrhea, and Overall Health in Early-Weaned Piglets
Authors: Ma Min , Chambers James K. , Uchida Kazuyuki , Ikeda Masanori , Watanabe Makiko , Goda Yuki , Yamanaka Daisuke , Takahashi Shin-Ichiro , Kuwahara Masayoshi , Li Junyou
Published: November 2021

This study assessed the feasibility of using a vegetable extract, MGM-P (quebracho tannin product), as an alternative to antibiotics for weaned piglets; it investigated MGM-P effects on growth performance, diarrhea, and overall health in early-weaned piglets. In total, 24 piglets were allocated to three treatment groups fed basal diets supplemented with 0, 0.2%, or 0.3% MGM-P for 20 days. The addition of 0.3% MGM-P to the diet of early-weaned piglets improved diarrhea incidence, hematological parameters, and intestinal mucosa structure. Furthermore, the addition of 0.2% or 0.3% MGM-P to the diet of early-weaned piglets did not affect their overall health. Importantly, MGM-P had no effects on average daily gain (ADG), average daily feed intake (ADFI), or feed conversion ratio (FCR). Gut morphology analysis showed that treatment with 0.3% MGM-P enhanced the jejunal villus height (p < 0.05) while reducing the ileal crypt depth (p < 0.05) and colon mucosal thickness (p < 0.05). Collectively, the findings suggested that the use of MGM-P as an alternative to dietary antibiotics could improve diarrhea incidence in early-weaned piglets without negative effects on growth performance or overall health.
An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II
Authors: Orsolini Morgan F. , Meyers Stuart A. , Dini Pouya
Published: November 2021

As the use of assisted reproductive technologies (ART) and in vitro embryo production (IVP) expand in the equine industry, it has become necessary to further our understanding of available semen selection techniques. This segment of our two-section review will focus on the selection of spermatozoa based on quality and sex for equine intracytoplasmic sperm injection (ICSI), as well as current and future developments in sperm sorting technologies. Ultimately, novel methods of semen selection will be assessed based on their efficacy in other species and their relevance and future application towards ARTs in the horse.
The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
Authors: Tvrdá Eva , Bučko Ondřej , Rojková Kristína , Ďuračka Michal , Kunová Simona , Kováč Ján , Benko Filip , Kačániová Miroslava
Published: November 2021

Bacteriospermia has become a serious factor affecting sperm quality in swine breeding, this is why antibiotics (ATBs) are a critical component of semen extenders. Due to ever-increasing antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available semen extenders to prevent a possible bacterial contamination of boar ejaculates. Three Androstar Plus extenders containing different combinations of antibiotics were used to process ejaculates from 30 healthy Duroc breeding boars. Androstar Plus without antibiotics was used as a control. The extended samples were stored at 17 °C for 72 h. Sperm motility, viability, mitochondrial activity, DNA integrity and oxidative profile of each extended sample were assessed following 24 h, 48 h and 72 h. Furthermore, selective media were used to quantify the bacterial load and specific bacterial species were identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicate that semen extenders enriched with ATBs ensured a significantly higher preservation of the sperm quality in comparison to the ATB-free control. The total bacterial count was significantly decreased in the extenders supplemented with ATBs (p < 0.001), however gentamycin alone was not effective enough against Gram-positive bacteria, while a few colonies of Enterococcus hirae, Bacillus subtilis and Corynebacterium spp. were present in the samples extended in the presence of a triple combination of ATBs. In conclusion, we may suggest that semen extenders enriched in antibiotics were not able to fully eliminate the bacteria present in the studied samples. Furthermore, selection of suitable antibiotics for semen extension should be accompanied by adequate hygiene standards during the collection and handling of boar ejaculates.
Management Factors Influence Animal Welfare and the Correlation to Infectious Diseases in Dairy Cows
Authors: Licitra Francesca , Perillo Laura , Antoci Francesco , Piccione Giuseppe , Giannetto Claudia , Salonia Rosario , Giudice Elisabetta , Monteverde Vincenzo , Cascone Giuseppe
Published: November 2021

The present study assessed dairy cow welfare through the application of the Italian National Animal Welfare Reference Center (CReNBA) checklist in 36 dairy farms located in Ragusa (Italy) subjected to two different management conditions, housing with free access to pasture (Group 1, farms n = 17) and indoor housing (Group 2, farms n = 19). Five areas of investigation were considered: Area A, “Farm management and personnel”; Area B, “Facilities and equipment”; Area C, “Animal-based measures”; Area D, “Inspection of microclimatic environmental conditions and alarm systems”; and Area E, “Biosecurity”. Blood samples were collected by coccygeal venipuncture from all animals (4081 cows). The specific antibodies against Mycobacterium avium subsp. paratuberculosis, Chlamydiophila abortus, Neospora caninum, bovine viral diarrhea virus, and the bovine herpesvirus were assessed by enzyme linked immunosorbent assay (ELISA) serological test. Group 1 (access to pasture) showed a lower value of percentage score recorded in Area A (p = 0.02) and E (p = 0.01) than Group 2 (indoor housing). Herpesvirus (Infectious bovine rhinotracheitis - IBR - detection of gB antibodies/IBR-gB) blood concentrations were higher in the cows housed indoor versus those with access to pasture (p = 0.01). Farm management and personnel (score A) was correlated with the level of bovine viral diarrhea virus (τ = 0.3754) and bovine-herpesvirus-specific antibodies (IBR-gB) (τ = 0.4159). “Biosecurity” percentage score showed a significant correlation with Chlamydiophila abortus (τ = −0.4621) in the cows with access to pasture and IBR-gB (τ = 0.3435) in the cows housed fully indoors. Group 2 showed a significantly reduced level of antibodies against Neospora caninum. In conclusion, differences in the welfare assessment score were observed in the “Farm management and personnel” and “Biosecurity” between the two management conditions. It had an effect on the prevalence of herpesvirus, which occurred more in cattle with access to pasture. Therefore, an accurate application of the checklist could be an instrument to prevent and control the spread of infections in farms.
A Matrilineal Study on the Origin and Genetic Relations of the Ecuadorian Pillareño Creole Pig Population through D-Loop Mitochondrial DNA Analysis
Authors: Vergara Amado Manuel Canales , Martínez Amparo Martínez , Bermejo Juan Vicente Delgado , Macri Martina , Nájera Pablo Rigoberto Andino , Duchi Nelson Antonio Duchi , Vargas Paula Alexandra Toalombo
Published: November 2021

Domestic pig breeds reached America on the second Columbus trip; from this date, Iberian pig genetic resources were disseminated throughout the continent, forming diverse creole breeds. These Ecuadorian Creole pigs are important for food production but have been genetically eroded since the introduction of transboundary breeds. In this study, we sought to characterize this erosion more thoroughly through mitochondrial DNA D-Loop analysis of Ecuadorian Pillareño Creole pigs from seven regions of Ecuador. To allow comparison, we also included in our analysis sequences from wild species, commercial lines, and domestic pigs, which were obtained from the NCBI GenBank database. Creole pigs’ population showed overall moderate Hd values and low π values, and a negative value of Tajima’s D was observed. The greatest differentiation from the Ecuadorian Pillareño Creole pigs was observed between Asian wild and Asian domestic pigs. The haplotype analysis revealed three different phylogenetic clades (A, E I, and E II) and 65 haplotypes. Ecuadorian Creole populations were grouped into nine haplotypes for Clade E I and E II, which have not previously been reported for Creole Pillareño populations. Our analysis indicates that in the establishment of Creole Pillareño pigs, individuals most likely separated from the Asian pig population and appear to be genetically influenced by European and Iberian populations raised in Spain.
Chronic Cholecystitis of Dogs: Clinicopathologic Features and Relationship with Liver
Authors: Mitsui Ikki , Ohtsuki Shigeaki , Uchida Kazuyuki
Published: November 2021

(1) Background: Chronic cholecystitis of dogs has not been vigorously investigated histopathologically. In addition, the relationship between gallbladder and liver diseases is not known. (2) Methods: We aimed to provide a hallmark for canine chronic cholecystitis using clinical data, histopathology, histochemistry, immunohistochemistry, and statistical analysis. (3) Results: Our investigation of 219 ultrasonographically abnormal surgically resected canine gallbladders revealed 189 cases (86.3%) of mucosal lymphoplasmacytic infiltration (chronic cholecystitis). Sludge, a gravity-dependent or nondependent fine granular hyperechoic material, was more prevalent (105/219, 47.9%) than mucocele (51/219, 23.2%) in this cohort. Mucosal lymphoid follicles were detected in 68/219 cases (31%), suggesting the influence of long-standing antigenic stimulation. Bacteria were histochemically detected in 41/60 (68.3%) of heavily inflamed gallbladders, 18/129 (14%) of lightly inflamed, and 3/18 (16.7%) of uninflamed gallbladders, suggesting a possible relationship between bacteria and chronic cholecystitis. Simultaneous liver biopsies revealed mild or no inflammation, changes consistent with primary portal vein hypoplasia, and mild hepatocellular degeneration. (4) Conclusions: Based on the results of our statistical analysis, we conclude that canine chronic cholecystitis is a long-standing inflammatory process of unknown (but possibly bacterial) etiology and that liver pathology is unlikely the cause of chronic cholecystitis in dogs.
Explaining the Unique Behavioral Characteristics of Elderly and Adults Based on Deep Learning
Authors: Byeon Yeong-Hyeon , Kim Dohyung , Lee Jaeyeon , Kwak Keun-Chang
Published: November 2021

In modern society, the population has been aging as the lifespan has increased owing to the advancement in medical technologies. This could pose a threat to the economic system and, in serious cases, to the ethics regarding the socially-weak elderly. An analysis of the behavioral characteristics of the elderly and young adults based on their physical conditions enables silver robots to provide customized services for the elderly to counter aging society problems, laying the groundwork for improving elderly welfare systems and automating elderly care systems. Accordingly, skeleton sequences modeling the changes of the human body are converted into pose evolution images (PEIs), and a convolutional neural network (CNN) is trained to classify the elderly and young adults for a single behavior. Then, a heatmap, which is a contributed portion of the inputs, is obtained using a gradient-weighted class activation map (Grad-CAM) for the classified results, and a skeleton-heatmap is obtained through a series of processes for the ease of analysis. Finally, the behavioral characteristics are derived through the difference matching analysis between the domains based on the skeleton-heatmap and RGB video matching analysis. In this study, we present the analysis of the behavioral characteristics of the elderly and young adults based on cognitive science using deep learning and discuss the examples of the analysis. Therefore, we have used the ETRI-Activity3D dataset, which is the largest of its kind among the datasets that have classified the behaviors of young adults and the elderly.
Hydrothermal Carbonization as Sustainable Process for the Complete Upgrading of Orange Peel Waste into Value-Added Chemicals and Bio-Carbon Materials
Authors: Satira Antonella , Paone Emilia , Bressi Viviana , Iannazzo Daniela , Marra Federica , Calabrò Paolo Salvatore , Mauriello Francesco , Espro Claudia
Published: November 2021

In this study, a simple and green protocol to obtain hydrochar and high-added value products, mainly 5-hydroxymethylfurfural (5-HMF), furfural (FU), levulinic acid (LA) and alkyl levulinates, by using the hydrothermal carbonization (HTC) of orange peel waste (OPW) is presented. Process variables, such as reaction temperature (180–300 °C), reaction time (60–300 min), biomass:water ratio and initial pH were investigated in order to find the optimum conditions that maximize both the yields of solid hydrochar and 5-HMF and levulinates in the bio-oil. Data obtained evidence that the highest yield of hydrochar is obtained at a 210 °C reaction temperature, 180 min residence time, 6/1 w/w orange peel waste to water ratio and a 3.6 initial pH. The bio-products distribution strongly depends on the applied reaction conditions. Overall, 180 °C was found to be the best reaction temperature that maximizes the production of furfural and 5-HMF in the presence of pure water as a reaction medium.
A Graph Theory-Based Method for Dynamic Modeling and Parameter Identification of 6-DOF Industrial Robots
Authors: Cheng Jun , Bi Shusheng , Yuan Chang , Chen Lin , Cai Yueri , Yao Yanbin
Published: November 2021

At present, the absolute positioning accuracy and control accuracy of industrial serial robots need to be improved to meet the accuracy requirements of precision manufacturing and precise control. An accurate dynamic model is an important theoretical basis for solving this problem, and precise dynamic parameters are the prerequisite for precise control. The research of dynamics and parameter identification can greatly promote the application of robots in the field of precision manufacturing and automation. In this paper, we study the dynamical modeling and dynamic parameter identification of an industrial robot system with six rotational DOF (6R robot system) and propose a new method for identifying dynamic parameters. Our aim is to provide an accurate mathematical description of the dynamics of the 6R robot and to accurately identify its dynamic parameters. First, we establish an unconstrained dynamic model for the 6R robot system and rewrite it to obtain the dynamic parameter identification model. Second, we establish the constraint equations of the 6R robot system. Finally, we establish the dynamic model of the constrained 6R robot system. Through the ADAMS simulation experiment, we verify the correctness and accuracy of the dynamic model. The experiments prove that the result of parameter identification has extremely high accuracy and the dynamic model can accurately describe the 6R robot system mathematically. The dynamic modeling method proposed in this paper can be used as the theoretical basis for the study of 6R robot system dynamics and the study of dynamics-based control theory.
Tailored Lace: Moldless Fabrication of 3D Bio-Composite Structures through an Integrative Design and Fabrication Process
Authors: Lehrecke August , Tucker Cody , Yang Xiliu , Baszynski Piotr , Dahy Hanaa
Published: November 2021

This research demonstrates an integrative computational design and fabrication workflow for the production of surface-active fibre composites, which uses natural fibres, revitalises a traditional craft, and avoids the use of costly molds. Fibre-reinforced polymers (FRPs) are highly tunable building materials, which gain efficiency from fabrication techniques enabling controlled fibre direction and placement in tune with load-bearing requirements. These techniques have evolved closely with industrial textile processes. However, increased focus on automation within FRP fabrication processes have overlooked potential key benefits presented by some lesser-known traditional techniques of fibre arrangement. This research explores the process of traditional bobbin lace-making and applies it in a computer-aided design and fabrication process of a small-scale structural demonstrator in the form of a chair. The research exposes qualities that can expand the design space of FRPs, as well as speculates about the potential automation of the process. In addition, Natural Fibre-Reinforced Polymers (NFRP) are investigated as a sustainable and human-friendly alternative to more popular carbon and glass FRPs.
Experimental and Finite Element Analysis of External ALC Panel Steel Frames with New Semi-Rigid Connector
Authors: Ding Kewei , Zong Da , Liu Yunlin , He Shulin , Shen Wanyu
Published: November 2021

In this paper, a new ALC panel connector was proposed. It has a good engineering economy and high fault tolerance. A quasistatic loading experiment was carried out to verify the feasibility of the external ALC panel steel frame under seismic loading. The test phenomena, hysteretic curve, skeleton curve, stiffness degradation, and energy dissipation of two sets of full-scale specimens were analyzed and discussed. Moreover, the simulation of pendulous Z-panel connectors with different thicknesses was carried out using ABAQUS software. The comparison reveals that the semi-rigid connection has a full hysteresis curve, good energy dissipation capacity, and a 15% increase in peak load capacity. Finally, similar results for different thicknesses in the use of pendulous Z-panel connectors reveal that using the 6 mm connector may be the most economical solution for engineering.