bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Si Substrate Preparation Procedure on Polarity of Self-Assembled GaN Nanowires on Si(111): Kelvin Probe Force Microscopy Studies
Authors: Sobanska Marta , Garro Núria , Klosek Kamil , Cros Ana , Zytkiewicz Zbigniew R.
Published: November 2020

The growth of GaN nanowires having a polar, wurtzite structure on nonpolar Si substrates raises the issue of GaN nanowire polarity. Depending on the growth procedure, coexistence of nanowires with different polarities inside one ensemble has been reported. Since polarity affects the optical and electronic properties of nanowires, reliable methods for its control are needed. In this work, we use Kelvin probe force microscopy to assess the polarity of GaN nanowires grown by plasma-assisted Molecular Beam Epitaxy on Si(111) substrates. We show that uniformity of the polarity of GaN nanowires critically depends on substrate processing prior to the growth. Nearly 18% of nanowires with reversed polarity (i.e., Ga-polar) were found on the HF-etched substrates with hydrogen surface passivation. Alternative Si substrate treatment steps (RCA etching, Ga-triggered deoxidation) were tested. However, the best results, i.e., purely N-polar ensemble of nanowires, were obtained on Si wafers thermally deoxidized in the growth chamber at ~1000 °C. Interestingly, no mixed polarity was found for GaN nanowires grown under similar conditions on Si(111) substrates with a thin AlOy buffer layer. Our results show that reversal of nanowires’ polarity can be prevented by growing them on a chemically uniform substrate surface, in our case on clean, in situ formed SiNx or ex situ deposited AlOy buffers.
Investigation on Hybrid Energy Storage Systems and Their Application in Green Energy Systems
Authors: Ma Chao-Tsung , Hsieh Chin-Lung
Published: November 2020

Power systems all over the world have been under development towards microgrids integrated with renewable energy-based distributed generation. Due to the intrinsic nature of output power fluctuations in renewable energy-based power generation, the use of proper energy storage systems and integrated real-time power and energy control schemes is an important basis of sustainable development of renewable energy-based distributed systems and microgrids. The aim of this paper is to investigate the characteristics and application features of an integrated compound energy storage system via simulation and a small-scale hardware system implementation. This paper first discusses the main components, working principles and operating modes of the proposed compound energy storage system. Then, a detailed design example composed of supercapacitors, batteries, and various controllers used in two typical application scenarios, peak demand shaving and power generation smoothing, of a grid-connected microgrid is systematically presented. Finally, an experimental setup with proper power converters and control schemes are implemented for the verification of the proposed control scheme. Both simulation and implementation results prove that the proposed scheme can effectively realize desired control objectives with the proposed coordinated control of the two energy storage devices.
Compressed-Sensing-Based Time–Frequency Representation for Disturbance Characterization of Maglev On-Board Distribution Systems
Authors: Xing Lu , Wen Yinghong , Xiao Shi , Zhang Jinbao , Zhang Dan
Published: November 2020

The frequency variating source, linear generator, and switching devices lead to dynamic characteristics of the low-frequency conducted emissions within maglev on-board distribution systems. To track the time-varying feature of these disturbances, a joint time–frequency representation combined adaptive optimal kernel with compressed sensing technique is proposed in this paper. The joint representation is based on Wigner–Ville distribution, and employs adaptive optimal kernel to remove undesirable cross terms. The compressed sensing technique is introduced to deal with the tradeoff between cross-component reduction and auto-component smearing faced by kernel-function-based bilinear time–frequency representation. The time–frequency aggregation and accuracy performance of joint time–frequency representation is quantified using Rényi entropy and l1-norm. To verify its performance in disturbance signature analysis for distribution systems and primarily characterize the low-frequency conducted emissions of maglev, a maglev on-board distribution system experimental platform is employed to extract the low-frequency disturbances which pose threats to the controlling system. Comparison with Wigner–Ville distribution demonstrates the joint time–frequency representation method outperforms in tracking time-varying and transient disturbances of maglev on-board distribution systems.
New Insights on a µm-Scale into the Transformation Process of CH4 Hydrates to CO2-Rich Mixed Hydrates
Authors: Pan Mengdi , Ismail Nur Aminatulmimi , Luzi-Helbing Manja , Koh Carolyn A. , Schicks Judith M.
Published: November 2020

The global occurrences of natural gas hydrates lead to the conclusion that tremendous amounts of hydrocarbons are bonded in these hydrate-bearing sediments, serving as a potential energy resource. For the release of the hydrate-bonded CH4 from these reservoirs, different production methods have been developed during the last decades. Among them, the chemical stimulation via injection of CO2 is considered as carbon neutral on the basis of the assumption that the hydrate-bonded CH4 is replaced by CO2. For the investigation of the replacement process of hydrate-bonded CH4 with CO2 on a µm-scale, we performed time-resolved in situ Raman spectroscopic measurements combined with microscopic observations, exposing the CH4 hydrates to a CO2 gas phase at 3.2 MPa and 274 K. Single-point Raman measurements, line scans and Raman maps were taken from the hydrate phase. Measurements were performed continuously at defined depths from the surface into the core of several hydrate crystals. Additionally, the changes in composition in the gas phase were recorded. The results clearly indicated the incorporation of CO2 into the hydrate phase with a concentration gradient from the surface to the core of the hydrate particle, supporting the shrinking core model. Microscopic observations, however, indicated that all the crystals changed their surface morphology when exposed to the CO2 gas. Some crystals of the initial CH4 hydrate phase grew or were maintained while at the same time other crystals decreased in sizes and even disappeared over time. This observation suggested a reformation process similar to Ostwald ripening rather than an exchange of molecules in already existing hydrate structures. The experimental results from this work are presented and discussed in consideration of the existing models, providing new insights on a µm-scale into the transformation process of CH4 hydrates to CO2-rich mixed hydrates.
Future Sensors for Smart Objects by Printing Technologies in Industry 4.0 Scenario
Authors: Borghetti Michela , Cantù Edoardo , Sardini Emilio , Serpelloni Mauro
Published: November 2020

Industry 4.0 has radically been transforming the production processes and systems with the adoption of enabling technologies, such as Internet of things (IoT), big data, additive manufacturing (AM), and cloud computing. In this context, sensors are essential to extract information about production, spare parts, equipment health, and environmental conditions necessary for improving many aspects of industrial processes (flexibility, efficiency, costs, etc.). Sensors should be placed everywhere (on machines, smart devices, objects, and tools) inside the factory to monitor in real-time physical quantities such as temperature, vibrations, deformations that could affect the production. Printed electronics (PE) offers techniques to produce unconventional sensor and systems or to make conventional objects “smart”. This work aims to analyze innovative PE technologies—inkjet printing and aerosol jet printing in combination with photonic curing—as manufacturing technologies for electronics and sensors to be integrated into objects, showing a series of sensors fabricated by PE as applications that will be adopted for smart objects and Industry 4.0.
Performance Assessment of Some Practical Loss of Excitation Detection Schemes Employing a Realistic Model
Authors: Hasani Abbas , Bak Claus Leth , da Silva Filipe Miguel Faria
Published: November 2020

Loss of excitation (LOE) relay is one of the most essential protection elements for synchronous generators in power plants. During the last few decades, several LOE detection methods have been proposed, while limited schemes such as admittance- and impedance-based ones have been adopted for industrial applications. This study investigates and compares the behavior of some practical LOE detection schemes through extensive simulation scenarios, and from the reliability, speed, and security points of view. The simulation scenarios are accomplished by using the real-time-digital-simulator, where the phase domain model of the synchronous generator is used to develop a realistic and typical power generation system. Employing such a system, different types of complete and partial LOE incidents can be applied according to IEEE Standard C37.102-2006, while the performance of any scheme can be assessed through accurate and realistic LOE scenarios.
Determination of the Theoretical and Actual Working Volume of a Hydraulic Motor
Authors: Sliwinski Pawel
Published: November 2020

A new methodology of determination of the theoretical and actual working volume of a hydraulic motor based on the characteristics of the delivered flow rate into hydraulic motor vs. the rotational speed at a constant pressure drop in the working chambers is described in this paper. A new method of describing the delivered flow rate into a motor per one shaft revolution as a nonlinear function of the pressure drop in the motor working chamber is proposed. The influence of the flowmeter location in the measurement system on the result of the theoretical and actual working volume calculation is described. It is shown that, in order to assess the energy losses (volumetric and mechanical) in the motor, the actual working volume must be a polynomial function (third degree) of its pressure drop in the working chambers. The result of the experimental tests of the satellite hydraulic motor confirmed the validity of the proposed method. The result of the calculation of the theoretical working volume of the motor according to the proposed method was compared with the results of calculations according to known methods.
Functional Diversification of the Dihydroflavonol 4-Reductase from Camellia nitidissima Chi. in the Control of Polyphenol Biosynthesis
Authors: Jiang Lina , Fan Zhengqi , Tong Ran , Zhou Xingwen , Li Jiyuan , Yin Hengfu
Published: November 2020

Plant secondary metabolism is complex in its diverse chemical composition and dynamic regulation of biosynthesis. How the functional diversification of enzymes contributes to the diversity is largely unknown. In the flavonoids pathway, dihydroflavonol 4-reductase (DFR) is a key enzyme mediating dihydroflavanol into anthocyanins biosynthesis. Here, the DFR homolog was identified from Camellia nitidissima Chi. (CnDFR) which is a unique species of the genus Camellia with golden yellow petals. Sequence analysis showed that CnDFR possessed not only conserved catalytic domains, but also some amino acids peculiar to Camellia species. Gene expression analysis revealed that CnDFR was expressed in all tissues and the expression of CnDFR was positively correlated with polyphenols but negatively with yellow coloration. The subcellular localization of CnDFR by the tobacco infiltration assay showed a likely dual localization in the nucleus and cell membrane. Furthermore, overexpression transgenic lines were generated in tobacco to understand the molecular function of CnDFR. The analyses of metabolites suggested that ectopic expression of CnDFR enhanced the biosynthesis of polyphenols, while no accumulation of anthocyanins was detected. These results indicate a functional diversification of the reductase activities in Camellia plants and provide molecular insights into the regulation of floral color.
FLNC Expression Level Influences the Activity of TEAD-YAP/TAZ Signaling
Authors: Knyazeva Anastasia , Khudiakov Aleksandr , Vaz Raquel , Muravyev Aleksey , Sukhareva Ksenia , Sejersen Thomas , Kostareva Anna
Published: November 2020

Filamin C (FLNC), being one of the major actin-binding proteins, is involved in the maintenance of key muscle cell functions. Inherited skeletal muscle and cardiac disorders linked to genetic variants in FLNC have attracted attention because of their high clinical importance and possibility of genotype-phenotype correlations. To further expand on the role of FLNC in muscle cells, we focused on detailed alterations of muscle cell properties developed after the loss of FLNC. Using the CRISPR/Cas9 method we generated a C2C12 murine myoblast cell line with stably suppressed Flnc expression. FLNC-deficient myoblasts have a significantly higher proliferation rate combined with an impaired cell migration capacity. The suppression of Flnc expression leads to inability to complete myogenic differentiation, diminished expression of Myh1 and Myh4, alteration of transcriptional dynamics of myogenic factors, such as Mymk and Myog, and deregulation of Hippo signaling pathway. Specifically, we identified elevated basal levels of Hippo activity in myoblasts with loss of FLNC, and ineffective reduction of Hippo signaling activity during myogenic differentiation. The latter was restored by Flnc overexpression. In summary, we confirmed the role of FLNC in muscle cell proliferation, migration and differentiation, and demonstrated for the first time the direct link between Flnc expression and activity of TEAD-YAP\TAZ signaling. These findings support a role of FLNC in regulation of essential muscle processes relying on mechanical as well as signaling mechanisms.
Role of the Common PRSS1-PRSS2 Haplotype in Alcoholic and Non-Alcoholic Chronic Pancreatitis: Meta- and Re-Analyses
Authors: Herzig Anthony F. , Génin Emmanuelle , Cooper David N. , Masson Emmanuelle , Férec Claude , Chen Jian-Min
Published: November 2020

The association between a common PRSS1-PRSS2 haplotype and alcoholic chronic pancreatitis (ACP), which was revealed by the first genome-wide association study of chronic pancreatitis (CP), has been consistently replicated. However, the association with non-ACP (NACP) has been controversial. Herein, we sought to clarify this basic issue by means of an allele-based meta-analysis of currently available studies. We then used studies informative for genotype distribution to explore the biological mechanisms underlying the association data and to test for gene-environment interaction between the risk haplotype and alcohol consumption by means of a re-analysis. A literature search was conducted to identify eligible studies. A meta-analysis was performed using the Review Manager software. The association between the risk genotypes and NACP or ACP was tested for the best-fitting genetic model. Gene-environment interaction was estimated by both case-only and multinomial approaches. Five and eight studies were employed for the meta-analysis of ACP and NACP findings, respectively. The risk allele was significantly associated with both ACP (pooled odds ratio (OR) 1.67, 95% confidence interval (CI) 1.56–1.78; p < 0.00001) and NACP (pooled OR 1.28, 95% CI 1.17–1.40; p < 0.00001). Consistent with a dosage effect of the risk allele on PRSS1/PRSS2 mRNA expression in human pancreatic tissue, both ACP and NACP association data were best explained by an additive genetic model. Finally, the risk haplotype was found to interact synergistically with alcohol consumption.
A High Quality Asian Genome Assembly Identifies Features of Common Missing Regions
Authors: Kim Jina , Sung Joohon , Han Kyudong , Lee Wooseok , Mun Seyoung , Lee Jooyeon , Bahk Kunhyung , Yang Inchul , Bae Young-Kyung , Kim Changhoon , Kim Jong-Il , Seo Jeong-Sun
Published: November 2020

The current human reference genome (GRCh38), with its superior quality, has contributed significantly to genome analysis. However, GRCh38 may still underrepresent the ethnic genome, specifically for Asians, though exactly what we are missing is still elusive. Here, we juxtaposed GRCh38 with a high-contiguity genome assembly of one Korean (AK1) to show that a part of AK1 genome is missing in GRCh38 and that the missing regions harbored ~1390 putative coding elements. Furthermore, we found that multiple populations shared some certain parts in the missing genome when we analyzed the “unmapped” (to GRCh38) reads of fourteen individuals (five East-Asians, four Europeans, and five Africans), amounting to ~5.3 Mb (~0.2% of AK1) of the total genomic regions. The recovered AK1 regions from the “unmapped reads”, which were the estimated missing regions that did not exist in GRCh38, harbored candidate coding elements. We verified that most of the common (shared by ≥7 individuals) missing regions exist in human and chimpanzee DNA. Moreover, we further identified the occurrence mechanism and ethnic heterogeneity as well as the presence of the common missing regions. This study illuminates a potential advantage of using a pangenome reference and brings up the need for further investigations on the various features of regions globally missed in GRCh38.
Multivariate Geostatistical Modeling and Risk Analysis of Beach Litter: A Case Study of Playa Blanca Beach, Chile
Authors: Maleki Mohammad , Soria Kevin
Published: November 2020

Beach litter is a worldwide problem that has several negative effects. A first step in preventing an environmental hazard is to determine and model the level of contamination. In this paper, geostatistical simulation is used to model two main forms of beach litter (cigarette butts and sharp items) in one of the most contaminated beaches in Antofagasta, Chile. A hundred realizations of cigarette butts and broken glass are generated to emulate their joint spatial distribution. The simulation results are used to classify the beach into different areas with respect to the risk of injury by broken glass and the level of contamination by cigarette butts. The models obtained can be used by local authorities in beach clean-up programs and by visitors to beaches in choosing the safest and cleanest areas. The results demonstrate the capability of geostatistical simulation algorithms to model different types of beach litter.
One Virus, Four Continents, Eight Countries: An Interdisciplinary and International Study on the Psychosocial Impacts of the COVID-19 Pandemic among Adults
Authors: Généreux Mélissa , Schluter Philip J. , Hung Kevin KC , Wong Chi Shing , Pui Yin Mok Catherine , O’Sullivan Tracey , David Marc D. , Carignan Marie-Eve , Blouin-Genest Gabriel , Champagne-Poirier Olivier , Champagne Éric , Burlone Nathalie , Qadar Zeeshan , Herbosa Teodoro , Ribeiro-Alves Gleisse , Law Ronald , Murray Virginia , Chan Emily Ying Yang , Pignard-Cheynel Nathalie , Salerno Sébastien , Lits Grégoire , d’Haenens Leen , Coninck David De , Matthys Koenraad , Roy Mathieu
Published: November 2020

The novel coronavirus disease 2019 (COVID-19) pandemic brought about several features that increased the sense of fear and confusion, such as quarantine and financial losses among other stressors, which may have led to adverse psychosocial outcomes. The influence of such stressors took place within a broader sociocultural context that needs to be considered. The objective was to examine how the psychological response to the pandemic varied across countries and identify which risk/protective factors contributed to this response. An online survey was conducted from 29 May 2020–12 June 2020, among a multinational sample of 8806 adults from eight countries/regions (Canada, United States, England, Switzerland, Belgium, Hong Kong, Philippines, New Zealand). Probable generalized anxiety disorder (GAD) and major depression episode (MDE) were assessed. The independent role of a wide range of potential factors was examined using multilevel logistic regression. Probable GAD and MDE were indicated by 21.0% and 25.5% of the respondents, respectively, with an important variation according to countries/regions (GAD: 12.2–31.0%; MDE: 16.7–32.9%). When considered together, 30.2% of the participants indicated probable GAD or MDE. Several factors were positively associated with a probable GAD or MDE, including (in descending order of importance) weak sense of coherence (SOC), lower age, false beliefs, isolation, threat perceived for oneself/family, mistrust in authorities, stigma, threat perceived for country/world, financial losses, being a female, and having a high level of information about COVID-19. Having a weak SOC yielded the highest adjusted odds ratio for probable GAD or MDE (3.21; 95% confidence interval (CI): 2.73–3.77). This pandemic is having an impact on psychological health. In some places and under certain circumstances, however, people seem to be better protected psychologically. This is a unique opportunity to evaluate the psychosocial impacts across various sociocultural backgrounds, providing important lessons that could inform all phases of disaster risk management.
Changes of Mood and Cognitive Performance before and after a 100 km Nighttime Ultramarathon Run
Authors: Krokosz Daniel , Bidzan-Bluma Ilona , Ratkowski Wojciech , Li Keqiang , Lipowski Mariusz
Published: November 2020

Ultramarathons are becoming an increasingly popular endurance sport. Year after year, the demands on athletes’ skills and endurance increase. Ultramarathons are particularly taxing on athletes’ psychological functioning. This study assessed the relationships between taking part in a nighttime ultramarathon and changes in mood and cognitive functioning. The study included 20 experienced runners aged 26–57 (M = 37.29; SD = 7.94) who had M = 7.08, SD = 5.41 (range 3–44) years of experience running. There were 18 men and 2 women. The mood states were measured twice, just before the start of the run and shortly after crossing the finish line, using the Polish version of the UMACL UWIST Mood Adjective Checklist by Mathews, Chamberlain, and Jones. To assess cognitive functioning, the Stroop Color and Word Test and “Forward digit span” subtest from the Wechsler Adult Intelligence Scale were used. We observed statistically significant changes in the mood of the runners: tense arousal, associated with the experienced stress, was significantly higher before the run than immediately after the finish. Moreover, we observed an improvement in cognitive functioning after finishing the 100 km run on both of the trials on the Stroop color word test and on the forward digit span test.
Thermal Personalities of Older People in South Australia: A Personas-Based Approach to Develop Thermal Comfort Guidelines
Authors: Bennetts Helen , Arakawa Martins Larissa , van Hoof Joost , Soebarto Veronica
Published: November 2020

An important consideration for future age-friendly cities is that older people are able to live in housing appropriate for their needs. While thermal comfort in the home is vital for the health and well-being of older people, there are currently few guidelines about how to achieve this. This study is part of a research project that aims to improve the thermal environment of housing for older Australians by investigating the thermal comfort of older people living independently in South Australia and developing thermal comfort guidelines for people ageing-in-place. This paper describes the approach fundamental for developing the guidelines, using data from the study participants’ and the concept of personas to develop a number of discrete “thermal personalities”. Hierarchical Cluster Analysis (HCA) was implemented to analyse the features of research participants, resulting in six distinct clusters. Quantitative and qualitative data from earlier stages of the project were then used to develop the thermal personalities of each cluster. The thermal personalities represent different approaches to achieving thermal comfort, taking into account a wide range of factors including personal characteristics, ideas, beliefs and knowledge, house type, and location. Basing the guidelines on thermal personalities highlights the heterogeneity of older people and the context-dependent nature of thermal comfort in the home and will make the guidelines more user-friendly and useful.
Awareness of Marketing of Heated Tobacco Products and Cigarettes and Support for Tobacco Marketing Restrictions in Japan: Findings from the 2018 International Tobacco Control (ITC) Japan Survey
Authors: Craig Lorraine V. , Yoshimi Itsuro , Fong Geoffrey T. , Meng Gang , Yan Mi , Mochizuki Yumiko , Tabuchi Takahiro , Thrasher James F. , Xu Steve S. , Quah Anne C. K. , Ouimet Janine , Sansone Genevieve , Chung-Hall Janet
Published: November 2020

Japan is one of the world’s largest cigarette markets and the top heated tobacco product (HTP) market. No forms of tobacco advertising, promotion, and sponsorship (TAPS) are banned under national law, although the industry has some voluntary TAPS restrictions. This study examines Japanese tobacco users’ self-reported exposure to cigarette and HTP marketing through eight channels, as well as their support for TAPS bans. Data are from the 2018 ITC Japan Survey, a cohort survey of adult exclusive cigarette smokers (n = 3288), exclusive HTP users (n = 164), HTP-cigarette dual users (n = 549), and non-users (n = 614). Measures of overall average exposure to the eight channels of cigarette and HTP advertising were constructed to examine differences in exposure across user groups and products. Dual users reported the highest exposure to cigarette and HTP advertising. Tobacco users (those who used cigarettes, HTPs, or both) reported higher average exposure to HTP compared to cigarette advertising, however non-users reported higher average exposure to cigarette compared to HTP advertising. Retail stores where tobacco or HTPs are sold were the most prevalent channel for HTP and cigarette advertising, reported by 30–43% of non-users to 66–71% of dual users. Non-users reported similar exposure to cigarette advertising via television and newspapers/magazines as cigarette smokers and dual users; however, advertising via websites/social media was lower among non-users and HTP users than among cigarette smokers and dual users (p < 0.05). Most respondents supported a ban on cigarette (54%) and HTP (60%) product displays in stores, and cigarette advertising in stores (58%).
Psychometric Properties and Reliability of the Referee Self-Efficacy Scale (REFS) in Volleyball Referees
Authors: Diotaiuti Pierluigi , Falese Lavinia , Mancone Stefania , Corrado Stefano , Mallia Luca , Zelli Arnaldo , Lucidi Fabio
Published: November 2020

Background: Volleyball officials require a combination of qualities, e.g., confidence, decisiveness, courage and mental toughness as very important attributes for their performance. Measurement of the self-efficacy of volleyball referees has not been studied with large samples; therefore, the aim of this study was to fill this gap in the research. Methods: Four-hundred and forty-five international volleyball referees participated in the study fulfilling the referee self-efficacy scale in the English version. Results: The confirmatory analysis verified the four-factor structure of the scale and its reliability in this specific sample of international volleyball referees. ANCOVA revealed a significant effect of the covariate “level of education” for all four dimensions of referees’ self-efficacy. Conclusions: Although English was not the mother tongue for most of the subjects in the sample, the scale was statistically reliable, and the items were easy to understand, thus making the tool very suitable to use for further studies on multilingual referees. The study also suggests enhancing the level of education of the officials because of its significant effect on the perceived self-efficacy during refereeing.
Land Suitability for Coffee (Coffea arabica) Growing in Amazonas, Peru: Integrated Use of AHP, GIS and RS
Authors: Salas López Rolando , Gómez Fernández Darwin , Silva López Jhonsy O. , Rojas Briceño Nilton B. , Oliva Manuel , Terrones Murga Renzo E. , Iliquín Trigoso Daniel , Barboza Castillo Elgar , Barrena Gurbillón Miguel Ángel
Published: November 2020

Peru is one of the world’s main coffee exporters, whose production is driven mainly by five regions and, among these, the Amazonas region. However, a combined negative factor, including, among others, climate crisis, the incidence of diseases and pests, and poor land-use planning, have led to a decline in coffee yields, impacting on the family economy. Therefore, this research assesses land suitability for coffee production (Coffea arabica) in Amazonas region, in order to support the development of sustainable agriculture. For this purpose, a hierarchical structure was developed based on six climatological sub-criteria, five edaphological sub-criteria, three physiographical sub-criteria, four socio-economic sub-criteria, and three restrictions (coffee diseases and pests). These were integrated using the Analytical Hierarchy Process (AHP), Geographic Information Systems (GIS) and Remote Sensing (RS). Of the Amazonas region, 11.4% (4803.17 km2), 87.9% (36,952.27 km2) and 0.7% (295.47 km2) are “optimal”, “suboptimal” and “unsuitable” for the coffee growing, respectively. It is recommended to orient coffee growing in 912.48 km2 of territory in Amazonas, which presents “optimal” suitability for coffee and is “unsuitable” for diseases and pests. This research aims to support coffee farmers and local governments in the region of Amazonas to implement new strategies for land management in coffee growing. Furthermore, the methodology used can be applied to assess land suitability for other crops of economic interest in Andean Amazonian areas.
Neither Excessive Nitric Oxide Accumulation nor Acute Hyperglycemia Affects the N-Acetylaspartate Network in Wistar Rat Brain Cells
Authors: Zyśk Marlena , Pikul Piotr , Kowalski Robert , Lewandowski Krzysztof , Sakowicz-Burkiewicz Monika , Pawełczyk Tadeusz
Published: November 2020

The N-acetylaspartate network begins in neurons with N-acetylaspartate production catalyzed by aspartate N-acetyltransferase from acetyl-CoA and aspartate. Clinical studies reported a significant depletion in N-acetylaspartate brain level in type 1 diabetic patients. The main goal of this study was to establish the impact of either hyperglycemia or oxidative stress on the N-acetylaspartate network. For the in vitro part of the study, embryonic rat primary neurons were treated by using a nitric oxide generator for 24 h followed by 6 days of post-treatment culture, while the neural stem cells were cultured in media with 25–75 mM glucose. For the in vivo part, male adult Wistar rats were injected with streptozotocin (65 mg/kg body weight, ip) to induce hyperglycemia (diabetes model) and euthanized 2 or 8 weeks later. Finally, the biochemical profile, NAT8L protein/Nat8l mRNA levels and enzymatic activity were analyzed. Ongoing oxidative stress processes significantly affected energy metabolism and cholinergic neurotransmission. However, the applied factors did not affect the N-acetylaspartate network. This study shows that reduced N-acetylaspartate level in type 1 diabetes is not related to oxidative stress and that does not trigger N-acetylaspartate network fragility. To reveal why N-acetylaspartate is reduced in this pathology, other processes should be considered.
PIMT Binding to C-Terminal Ala459 of CAIX Is Involved in Inside-Out Signaling Necessary for Its Catalytic Activity
Authors: Simko Veronika , Belvoncikova Petra , Csaderova Lucia , Labudova Martina , Grossmannova Katarina , Zatovicova Miriam , Kajanova Ivana , Skultety Ludovit , Barathova Monika , Pastorek Jaromir
Published: November 2020

Human carbonic anhydrase IX (CAIX), a unique member of the α carbonic anhydrase family, is a transmembrane glycoprotein with high enzymatic activity by which CAIX contributes to tumorigenesis through pH regulation. Due to its aberrant expression, CAIX is considered to be a marker of tumor hypoxia and a poor prognostic factor of several human cancers. Hypoxia-activated catalytic function of CAIX is dependent on posttranslational modification of its short intracellular domain. In this work, we have identified that C-terminal Ala459 residue, which is common across CAIX of various species as well as additional transmembrane isoforms, plays an important role in CAIX activation and in pH regulation. Moreover, structure prediction I-TASSER analysis revealed involvement of Ala459 in potential ligand binding. Using tandem mass spectrometry, Protein-L-isoaspartyl methyltransferase (PIMT) was identified as a novel interacting partner, further confirmed by an in vitro pulldown assay and an in situ proximity ligation assay. Indeed, suppression of PIMT led to increased alkalinization of culture media of C33a cells constitutively expressing CAIX in hypoxia. We suggest that binding of PIMT represents a novel intracellular signal required for enzymatic activity of CAIX with a potential unidentified downstream function.
Novel Therapies for Relapsed or Refractory Diffuse Large B-Cell Lymphoma
Authors: Harris Leonard Jeff , Patel Kruti , Martin Michael
Published: November 2020

The most common type of non-Hodgkin lymphoma in adults is diffuse large B-cell (DLBCL). There is a historical unmet need for more effective therapies in the 2nd and 3rd line setting. Emerging immunochemotherapies have shown activity in small studies of heavily pre-treated patients with prolonged remissions achieved in some patients. Anti-CD19 CAR (chimeric antigen receptor) T cells are potentially curative in the 3rd line and beyond setting and are under investigation in earlier lines of therapy. Antibody-drug conjugates (ADC’s) such as polatuzumab vedotin targeting the pan-B-cell marker CD79b has proven effectiveness in multiply-relapsed DLBCL patients. Tafasitamab (MOR208) is an anti-CD19 monoclonal antibody producing prolonged remissions when combined with Lenalidomide (LEN) in patients who were not candidates for salvage chemotherapy or autologous stem cell transplant. Selinexor, an oral, small-molecule selective inhibitor of XPO1-mediated nuclear export (SINE), demonstrated prolonged activity against heavily-pretreated DLBCL without cumulative toxicity and is being investigated as part of an oral, chemotherapy-free regimen for relapsed aggressive lymphoma. This article reviews current strategies and novel therapies for relapsed/refractory DLBCL.
Variants of STAR, AMH and ZFPM2/FOG2 May Contribute towards the Broad Phenotype Observed in 46,XY DSD Patients with Heterozygous Variants of NR5A1
Authors: Martínez de LaPiscina Idoia , Mahmoud Rana AA , Sauter Kay-Sara , Esteva Isabel , Alonso Milagros , Costa Ines , Rial-Rodriguez Jose Manuel , Rodríguez-Estévez Amaia , Vela Amaia , Castano Luis , Flück Christa E.
Published: November 2020

Variants of NR5A1 are often found in individuals with 46,XY disorders of sex development (DSD) and manifest with a very broad spectrum of clinical characteristics and variable sex hormone levels. Such complex phenotypic expression can be due to the inheritance of additional genetic hits in DSD-associated genes that modify sex determination, differentiation and organ function in patients with heterozygous NR5A1 variants. Here we describe the clinical, biochemical and genetic features of a series of seven patients harboring monoallelic variants in the NR5A1 gene. We tested the transactivation activity of novel NR5A1 variants. We additionally included six of these patients in a targeted diagnostic gene panel for DSD and identified a second genetic hit in known DSD-causing genes STAR, AMH and ZFPM2/FOG2 in three individuals. Our study increases the number of NR5A1 variants related to 46,XY DSD and supports the hypothesis that a digenic mode of inheritance may contribute towards the broad spectrum of phenotypes observed in individuals with a heterozygous NR5A1 variation.
Tunable Supramolecular Chirogenesis in the Self-Assembling of Amphiphilic Porphyrin Triggered by Chiral Amines
Authors: Savioli Marco , Stefanelli Manuela , Magna Gabriele , Zurlo Francesca , Caso Maria Federica , Cimino Rita , Goletti Claudio , Venanzi Mariano , Di Natale Corrado , Paolesse Roberto , Monti Donato
Published: November 2020

Supramolecular chirality is one of the most important issues in different branches of science and technology, as stereoselective molecular recognition, catalysis, and sensors. In this paper, we report on the self-assembly of amphiphilic porphyrin derivatives possessing a chiral information on the periphery of the macrocycle (i.e., D- or L-proline moieties), in the presence of chiral amines as co-solute, such as chiral benzylamine derivatives. The aggregation process, steered by hydrophobic effect, has been studied in aqueous solvent mixtures by combined spectroscopic and topographic techniques. The results obtained pointed out a dramatic effect of these ligands on the morphology and on the supramolecular chirality of the final self-assembled structures. Scanning electron microscopy topography, as well as fluorescence microscopy studies revealed the formation of rod-like structures of micrometric size, different from the fractal structures formerly observed when the self-assembly process is carried out in the absence of chiral amine co-solutes. On the other hand, comparative experiments with an achiral porphyrin analogue strongly suggested that the presence of the prolinate moiety is mandatory for the achievement of the observed highly organized suprastructures. The results obtained would be of importance for unraveling the intimate mechanisms operating in the selection of the homochirality, and for the preparation of sensitive materials for the detection of chiral analytes, with tunable stereoselectivity and morphology.
Salivary Stress/Immunological Markers in Crohn’s Disease and Ulcerative Colitis
Authors: Finamore Alberto , Peluso Ilaria , Cauli Omar
Published: November 2020

There is continuous and growing interest in research into new alternatives to standard biomarkers to detect and follow-up disease, reducing physical and psychological stress in patients needing regular and invasive medical examinations for the evaluation of pathologies, including inflammatory bowel diseases (IBD). Saliva is one of the most promising body fluids in the research of new biomarkers, thanks to the large number of molecules it contains. Many molecules present in saliva are often directly correlated to their concentration in the blood but may be affected by the condition of the oral cavity. This means that a careful selection of a specific biomarker is required for each pathology, especially pathologies such as IBD, which may induce inflammation in the oral cavity. Here, we analyze the currently used and the proposed new salivary biomarkers (i.e., calprotectin, cytokines, IgA, cortisol, and oxidative stress markers) for the detection and follow-up of the main subtypes of IBD, known as ulcerative colitis and Crohn’s disease.
Use of Semiochemicals for the Management of the Redbay Ambrosia Beetle
Authors: Martini Xavier , Hughes Marc A. , Conover Derrick , Smith Jason
Published: November 2020

This review highlights current advances in the management of the redbay ambrosia beetle, Xyleborus glabratus, a primary vector of the pathogenic fungus, Raffaelea lauricola, that causes laurel wilt. Laurel wilt has a detrimental effect on forest ecosystems of southeastern USA, with hundreds of millions of Lauraceae deaths. Currently, preventive measures mostly focus on infected-tree removal to potentially reduce local beetle populations and/or use of preventative fungicide applications in urban trees. Use of semiochemicals may offer an opportunity for the management of X. glabratus. Research on attractants has led to the development of α-copaene lures that are now the accepted standards for X. glabratus sampling. Research conducted on repellents first included methyl salicylate and verbenone and attained significant reduction in the number of X. glabratus captured on redbay and swamp bay trees treated with verbenone. However, the death rate of trees protected with verbenone, while lower compared to untreated trees, is still high. This work underscores the necessity of developing new control methods, including the integration of repellents and attractants into a single push-pull system.
Assessment of a Novel Adult Mass-Rearing Cage for Aedes albopictus (Skuse) and Anopheles arabiensis (Patton)
Authors: Maïga Hamidou , Mamai Wadaka , Bimbilé Somda Nanwintoum Séverin , Wallner Thomas , Poda Bèwadéyir Serge , Salvador-Herranz Gustavo , Argiles-Herrero Rafael , Yamada Hanano , Bouyer Jérémy
Published: November 2020

Successful implementation of the sterile insect technique (SIT) against Aedes albopictus and Anopheles arabiensis relies on a continuous supply of sterile males. To meet this requirement, optimization of the mass-rearing techniques is needed. This study, therefore, aims to assess a new mass-rearing cage (MRC) in terms of egg production efficiency and egg hatch rate (quality). In addition, adult survival was evaluated based on a cage adult-index for Ae. albopictus. Moreover, the cage’s suitability for use in mass An. arabiensis egg production was compared to that of the FAO/IAEA Anopheles reference cage. In Ae. albopictus rearing, the new MRC produced 1,112,110 eggs per cage following six blood meals, with minimum loss of eggs in the egging water. Furthermore, the adult index gave a good proxy of daily mortality rates in Ae. albopictus. In An. arabiensis rearing, about 130,000 eggs per egg batch were collected both from the new and the reference MRC. These findings suggest that the new MRC prototype is efficient in terms of egg production and can be used for mass-rearing in SIT programs targeting Ae. albopictus as well as An. arabiensis. The adult index was also positively validated for the detection of unusual mortality rates in Ae. albopictus mass-rearing facilities. Overall, the new MRC has shown several advantages; however, further improvements are necessary to minimize escapes during the egg collection processes.
Updated Strategy and Scope of the Multidisciplinary Scientific Journal J
Authors: Vazquez Franck
Published: November 2020

The journal J was launched at the beginning of 2018 [...]
The Activity of N-acetyl-β-hexosaminidase in the Blood, Urine, Cerebrospinal Fluid and Vitreous Humor Died People Due to Alcohol Intoxication
Authors: Ptaszyńska-Sarosiek Iwona , Chojnowska Sylwia , Szajda Sławomir Dariusz , Szeremeta Michał , Wardaszka Zofia , Cwalina Urszula , Niemcunowicz-Janica Anna , Waszkiewicz Napoleon
Published: November 2020

Background: The article aimed to assess the activity of the hexosaminidase (HEX) and its HEX A and HEX B isoenzymes in persons who suddenly died due to ethanol poisoning and explain the cause of their death. Methods: The research involved two groups of the deceased group A—22 people (20 males, 2 females; the average age 46 years) who died due to alcohol intoxication (with the blood alcohol content of 4‰ and above in all biological materials at the time of death—blood, urine, cerebrospinal fluid, and vitreous humor), and group B—30 people (22 males, 8 females; the average age 54 years), who died suddenly due to other reasons than alcohol. Results: The highest activity of the HEX was found in the serum of A and B groups. A significantly lower activity of HEX, HEX A, and HEX B was observed in the urine of group A in comparison to the sober decedents. Conclusion: The lower activity of HEX and its isoenzymes in the dead’s urine due to ethanol poisoning may suggest its usefulness as a potential marker of harmful alcohol drinking. Damage done to the kidneys by ethanol poisoning may be one of the possible mechanisms leading to death. Kidneys may be damaged intravitally via the inflammatory agent. Thus, it is necessary to conduct further research to evaluate the diagnostic usefulness of exoglycosidases while determining the death mechanisms of people who lost their lives due to ethanol poisoning.
Videocapillaroscopy of the Oral Mucosa in Patients with Diabetic Foot: Possible Diagnostic Role of Microangiopathic Damage?
Authors: Scardina Giuseppe A. , Guercio Giovanni , Valenti Cesare F. , Tegolo Domenico , Messina Pietro
Published: November 2020

Introduction: Diabetic foot represents one of the most serious and expensive complications of diabetes and is subject to a high percentage of amputations that are almost always preceded by ulcers ascribable to neuropathy and/or vasculopathy. Videocapillaroscopy (VCS) can be a valuable aid in order to uncover morpho-structural anomalies in the vascular bed, both at the level of the oral mucosa and at the level of the terminal vessels of the lower limb. Materials and methods: Sixty subjects divided into 4 groups were enrolled: 15 healthy subjects; 15 patients with diabetes for more than 10 years without ulcerative foot lesions; 15 patients with neuropathic diabetic foot (clinical diagnosis, MDNS); 15 patients with ischemic diabetic foot (clinical diagnosis, ABI, lower limb doppler). A complete videocapillaroscopic mapping of the oral mucosa was carried out on each patient. The areas investigated were: labial mucosa, the retro-commissural region of the buccal mucosa, and the vestibular masticatory mucosa (II and V sextant). Results: The analysis of the morphological and densitometric characteristics of the capillaries revealed the following: a significant reduction in capillary density in neuropathic (mean ± SD 7.32 ± 2.1) and ischemic patients (mean ± SD 4.32 ± 3.2) compared to the control group of patients (both diabetic mean ± SD 12.98 ± 3.1 and healthy mean ± SD 19.04 ± 3.16) (ANOVA test and Bonferroni t test p < 0.05); a reduction in the average length of the capillaries and a significant increase in tortuosity (ANOVA test and Bonferroni t test p < 0.05). In the neuropathic patients, a recurrent capillaroscopic pattern that we defined as “sun” was found, with capillaries arranged radially around an avascular area. Conclusions: The data obtained from this preliminary study suggest a potential diagnostic role of oral capillaroscopy in the early and subclinical identification of microangiopathic damage in patients with diabetic foot.
Advances in Machine Learning Detecting Changeover Processes in Cyber Physical Production Systems
Authors: Engelmann Bastian , Schmitt Simon , Miller Eddi , Bräutigam Volker , Schmitt Jan
Published: November 2020

The performance indicator, Overall Equipment Effectiveness (OEE), is one of the most important ones for production control, as it merges information of equipment usage, process yield, and product quality. The determination of the OEE is oftentimes not transparent in companies, due to the heterogeneous data sources and manual interference. Furthermore, there is a difference in present guidelines to calculate the OEE. Due to a big amount of sensor data in Cyber Physical Production Systems, Machine Learning methods can be used in order to detect several elements of the OEE by a trained model. Changeover time is one crucial aspect influencing the OEE, as it adds no value to the product. Furthermore, changeover processes are fulfilled manually and vary from worker to worker. They always have their own procedure to conduct a changeover of a machine for a new product or production lot. Hence, the changeover time as well as the process itself vary. Thus, a new Machine Learning based concept for identification and characterization of machine set-up actions is presented. Here, the issue to be dealt with is the necessity of human and machine interaction to fulfill the entire machine set-up process. Because of this, the paper shows the use case in a real production scenario of a small to medium size company (SME), the derived data set, promising Machine Learning algorithms, as well as the results of the implemented Machine Learning model to classify machine set-up actions.