bluuebunny/crossref_metadata_2025 huggingface dataset:
REDUPLIKASI VERBA BAHASA MUNA DIALEK KIOKO
Authors: Mustopa Ali , Abroor Awalul
Published: September 2024

Penelitian ini bertujuan mendeskripsikan reduplikasi verba bahasa Muna dialek Kioko. Penelitian ini menggunakan metode deskriptif kualitatif. Data yang dikumpulkan kemudian dianalisis melalui teknik top down (analisis menurun) untuk menganalisis bentuk reduplikasi verba bahasa Muna dialek Kioko, teknik semantis untuk menganalisis makna reduplikasi verba bahasa Muna dialek Kioko, dan teknik unsur pilar langsung untuk menganalisis fungsi reduplikasi verba bahasa Muna dialek Kioko. Reduplikasi verba bahasa Muna dialek Kioko dapat ditemukan tiga jenis reduplikasi yaitu reduplikasi utuh,  reduplikasi sebagian yaitu terjadinya pengulangan didua atau tiga suku kata pertama dan reduplikasi berafiks. Makna reduplikasi verba bahasa Muna dialek Kioko yaitu, (1) penuturan makna ‘banyak’, (2) tuturan makna ‘suruhan atau permintaan’, (3) tuturan makna ‘perbuatan tersebut ialah pada bentuk dasar yang berulang-ulang’, (4) tuturan makna ‘perbuatan ialah bentuk dasar yang dilakukan dengan santainya’, (5) tuturan mengenai perbuatan tersebut ialah dilakukan oleh dua belah pihak dan saling mengenai’, (6) tuturan makna yang menyatakan intensitas perasaan yang sebenarnya makna dari bentuk dasar. Fungsi reduplikasi ada dua yaitu fungsi gramatikal dan fungsi semantis. Fungsi reduplikasi verba bahasa Muna dialek Kioko ada yang bersifat derivasional (yang mengubah kelas kata) dan ada pula yang tidak merubah kelas kata ketika afiks berdistribusi dengan bentuk dasar sehingga membentuk kata ulang. Reduplikasi berafiks dalam reduplikasi verba bahasa Muna dialek Kioko yaitu prefiks (nee-, me-, ae-, po-, dan poka-), infiks (-um), sufiks (-ci, -e, -ane, dan –anda), konfiks (po-+-li dan po-+-ane), kombinasi afiks/infiks-sufiks (-um –no).
 Kata Kunci: Reduplikasi, bahasa Muna, dialek Kioko
First-trimester predictive models for adverse pregnancy outcomes—a base for implementation of strategies to prevent cardiovascular disease development
Authors: Hromadnikova Ilona , Kotlabova Katerina , Krofta Ladislav
Published: September 2024

Introduction

This study aimed to establish efficient, cost-effective, and early predictive models for adverse pregnancy outcomes based on the combinations of a minimum number of miRNA biomarkers, whose altered expression was observed in specific pregnancy-related complications and selected maternal clinical characteristics.

Methods

This retrospective study included singleton pregnancies with gestational hypertension (GH, n = 83), preeclampsia (PE, n = 66), HELLP syndrome (n = 14), fetal growth restriction (FGR, n = 82), small for gestational age (SGA, n = 37), gestational diabetes mellitus (GDM, n = 121), preterm birth in the absence of other complications (n = 106), late miscarriage (n = 34), stillbirth (n = 24), and 80 normal term pregnancies. MiRNA gene expression profiling was performed on the whole peripheral venous blood samples collected between 10 and 13 weeks of gestation using real-time reverse transcription polymerase chain reaction (RT-PCR).

Results

Most pregnancies with adverse outcomes were identified using the proposed approach (the combinations of selected miRNAs and appropriate maternal clinical characteristics) (GH, 69.88%; PE, 83.33%; HELLP, 92.86%; FGR, 73.17%; SGA, 81.08%; GDM on therapy, 89.47%; and late miscarriage, 84.85%). In the case of stillbirth, no addition of maternal clinical characteristics to the predictive model was necessary because a high detection rate was achieved by a combination of miRNA biomarkers only [91.67% cases at 10.0% false positive rate (FPR)].

Conclusion

The proposed models based on the combinations of selected cardiovascular disease-associated miRNAs and maternal clinical variables have a high predictive potential for identifying women at increased risk of adverse pregnancy outcomes; this can be incorporated into routine first-trimester screening programs. Preventive programs can be initiated based on these models to lower cardiovascular risk and prevent the development of metabolic/cardiovascular/cerebrovascular diseases because timely implementation of beneficial lifestyle strategies may reverse the dysregulation of miRNAs maintaining and controlling the cardiovascular system.
Web-based science communication at Research Institute level: balancing dissemination, dialogue and promotion in a major Italian scientific institution
Authors: Giuffredi Rita , Grasso Valentina , L’Astorina Alba
Published: September 2024

This study examines the online communication practices of research institutes affiliated with the Italian National Research Council. The analysis employs a combination of quantitative and qualitative methods to evaluate whether these institutes leverage digital technologies to transition from a unidirectional, strategic approach to communication towards a more dialogic and engaging approach with the public. Specifically, the study analyses the institutes’ websites, assessing their use of communication tools, content, target audience, knowledge flows, and contextualization of communication activities, while also highlighting disciplinary-based differences. Although recent research identified Research Institutes’ level as a promising ground for research communication to flourish, our study suggests that, on average, they still employ digital technologies in the same way as traditional media, disseminating information about institutional advancements rather than fostering interactive dialogue. Communication styles remain grounded in Dissemination and Strategic models, which restricts the ability of research institutes to develop a dynamic and transformative relationship with society.
Archaeological evidence of anthropogenic burning for food production in forested uplands of the Grand Canyon province, northern Arizona
Authors: Sullivan Alan P. , McNamee Calla , Wendel Martha , Mink Philip B. , Allen Susan E.
Published: September 2024

Despite convincing archaeological arguments about the global effects of human pyrogeography and their evolutionary significance, many of the implicated data sources are unavailable in research contexts that lack significant accumulations of charcoal or stands of fire-scarred trees. In view of the strong likelihood that hominins routinely ignited small, low-intensity landscape fires for millennia, we explore the role of cultural burning for food-production in an area of the American Southwest where anthropogenic fire has not been considered. To illustrate the virtues of a multidisciplinary approach, informed by Formation Theory and time perspectivism, we focus on the returns from macrobotanical and palynological analyses of samples recovered from a variety of archaeological and geoarchaeological contexts in the Upper Basin, a landform located south of the Grand Canyon in northern Arizona. Previous archaeobotanical studies of samples recovered from archaeological sites (ca. AD 500–1500) in the basin's pinyon-juniper woodlands are dominated by amaranth, chenopodium, and other economic ruderals. These findings support the “fire foodway” model that posits prehistoric Indigenous populations of the Upper Basin depended on these fire-following wild plants, rather than maize, by harvesting their abundant seeds and leaves from production locations that were created by low-intensity understory fires. In this paper, we present the results of new studies of archaeobotanical remains recovered from cut-back terraces and sedimentary contexts that (i) expand the evidence base for the fire-foodway model, (ii) provide a basis for proposing several types of prehistoric cultural burning practices, and (iii) introduce the outlines of the ruderal seed-bed hypothesis. Combined, these findings provide a new archaeological perspective on upland subsistence practices in the northern American Southwest. Our study also highlights biases of modern vegetation surveys that do not include archaeological data, and contributes to an appreciation of the extent to which biodiversity has declined because of widespread fire exclusion.
Risk factors for cardiometabolic health in Ghana: Cardiometabolic Risks Study Protocol-APTI Project
Authors: Hormenu Thomas , Salifu Iddrisu , Antiri Ebenezer Oduro , Paku Juliet Elikem , Arthur Aaron Rudolf , Nyane Benjamin , Ableh Eric Awlime , Gablah Augustine Mac-Hubert , Banson Cecil , Amoah Samuel , Ishimwe Marie Consolatrice Sage , Mugeni Regine
Published: September 2024

Introduction

Cardiometabolic diseases are rapidly becoming primary causes of death in developing countries, including Ghana. However, risk factors for these diseases, including obesity phenotype, and availability of cost-effective diagnostic criteria are poorly documented in an African-ancestry populations in their native locations. The extent to which the environment, occupation, geography, stress, and sleep habits contribute to the development of Cardiometabolic disorders should be examined.

Purpose

The overall goal of this study is to determine the prevalence of undiagnosed diabetes, prediabetes, and associated cardiovascular risks using a multi-sampled oral glucose tolerance test. The study will also investigate the phenotype and ocular characteristics of diabetes and prediabetes subgroups, as well as determine if lifestyle changes over a one-year period will impact the progression of diabetes and prediabetes.

Methods and analysis

The study employs a community-based quasi-experimental design, making use of pre- and post-intervention data, as well as a questionnaire survey of 1200 individuals residing in the Cape Coast metropolis to ascertain the prevalence and risk factors for undiagnosed diabetes and prediabetes. Physical activity, dietary habits, stress levels, sleep patterns, body image perception, and demographic characteristics will be assessed. Glucose dysregulation will be detected using oral glucose tolerance test, fasting plasma glucose, and glycated hemoglobin. Liver and kidney function will also be assessed. Diabetes and prediabetes will be classified using the American Diabetes Association criteria. Descriptive statistics, including percentages, will be used to determine the prevalence of undiagnosed diabetes and cardiovascular risks. Inferential statistics, including ANOVA, t-tests, chi-square tests, ROC curves, logistic regression, and linear mixed model regression will be used to analyze the phenotypic variations in the population, ocular characteristics, glycemic levels, sensitivity levels of diagnostic tests, etiological cause of diabetes in the population, and effects of lifestyle modifications, respectively. Additionally, t-tests will be used to assess changes in glucose regulation biomarkers after lifestyle modifications.

Ethics and dissemination

Ethics approval was granted by the Institutional Review Board of the University of Cape Coast, Ghana (UCCIRB/EXT/2022/27). The findings will be disseminated in community workshops, online learning platforms, academic conferences and submitted to peer-reviewed journals for publication.
Case report: A novel TLK2 variant with a neuropsychiatric phenotype from a Chinese family
Authors: Huang Hongmei , Qian Yue , Yang Chenlu , Li Shijie
Published: September 2024

Background

Tousled-like kinase 2 (TLK2) gene variant-related neurodevelopmental disorder was recently described. The haploinsufficiency of TLK2 was considered the most likely underlying disease mechanism, leading to a consistent neurodevelopmental phenotype. So far, only four studies, conducted on 49 patients from North America and Europe, have been reported.

Case presentation

In this study, we reported a Chinese family with a TLK2-related neuropsychiatric phenotype. The proband, a boy aged 2 years and 6 months, presented with temper tantrums, mood lability, aggressiveness, congenital astigmatism, and distinctive facial dysmorphism. Whole-exome sequencing identified a novel heterozygous variation in TLK2 gene (c.49dupG, p. E17Gfs*10) in them. His father carried the same TLK2 gene variant and exhibited anxiety and irritability. The parental grandparents and other family members had no such variation. Moreover, the proband was found to have global developmental delay, autism-like symptoms, and mild elevated homo-vanillic acid (HVA) and 2,3-dihydroxy-2-methylbutyric acid levels tested in urine.

Conclusion

Herein, we identified a novel TLK2 variant from a Chinese family and reported a new neuropsychiatric phenotype. This study also expanded the genotype profile of the newly defined TLK2-related neurodevelopmental disorder.
Host long noncoding RNAs in bacterial infections
Authors: Cheng Yong , Liang Yurong , Tan Xuejuan , Liu Lin
Published: September 2024

Bacterial infections remain a significant global health concern, necessitating a comprehensive understanding of the intricate host−pathogen interactions that play a critical role in the outcome of infectious diseases. Recent investigations have revealed that noncoding RNAs (ncRNAs) are key regulators of these complex interactions. Among them, long noncoding RNAs (lncRNAs) have gained significant attention because of their diverse regulatory roles in gene expression, cellular processes and the production of cytokines and chemokines in response to bacterial infections. The host utilizes lncRNAs as a defense mechanism to limit microbial pathogen invasion and replication. On the other hand, some host lncRNAs contribute to the establishment and maintenance of bacterial pathogen reservoirs within the host by promoting bacterial pathogen survival, replication, and dissemination. However, our understanding of host lncRNAs in the context of bacterial infections remains limited. This review focuses on the impact of host lncRNAs in shaping host−pathogen interactions, shedding light on their multifaceted functions in both host defense and bacterial survival, and paving the way for future research aimed at harnessing their regulatory potential for clinical applications.
The relationship between anesthesia, surgery and postoperative immune function in cancer patients: a review
Authors: Guo Rui , Yang Wen-wen , Zhong Mao-lin , Rao Pan-guo , Luo Xin , Liao Bao-zhen , Lei Xing-heng , Ye Jun-ming
Published: September 2024

This review comprehensively examines the impact of anesthesia and surgical interventions on the immune function of cancer patients postoperatively. Recent studies have shown that surgery and its accompanying anesthesia management can significantly influence immune function in cancer patients, potentially affecting their prognosis. This review synthesizes clinical studies and basic research to summarize the specific effects of anesthesia methods, drugs, postoperative analgesia, intraoperative transfusion, surgical techniques, and trauma extent on the immune function of cancer patients post-surgery. Additionally, this review discusses optimization strategies based on current research, aiming to refine anesthesia and surgical management to maximize the preservation and enhancement of postoperative immune function in cancer patients, with the potential to improve clinical outcomes.
The role of cGAS-STING signaling in the development and therapy of head and neck squamous cell carcinoma
Authors: Shao Chengze , Chen Jiawen , Qiang Bi , Ye Junmei , Yan Fangrong , Zhu Yongbo
Published: September 2024

The cGAS-STING signaling pathway plays a critical role in innate immunity and defense against viral infections by orchestrating intracellular and adaptive immune responses to DNA. In the context of head and neck squamous cell carcinoma (HNSCC), this pathway has garnered significant attention due to its potential relevance in disease development and progression. HNSCC is strongly associated with risk factors such as smoking, heavy alcohol consumption, and human papillomavirus (HPV) infection. The presence or absence of HPV in HNSCC patients has been shown to have a profound impact on patient survival and prognosis, possibly due to the distinct biological characteristics of HPV-associated tumors. This review aims to provide a comprehensive overview of the current therapeutic approaches and challenges in HNSCC management, as well as the involvement of cGAS-STING signaling and its potential in the therapy of HNSCC. In addition, by advancing the present understanding of the mechanisms underlying this pathway, Activation of cGAS–STING-dependent inflammatory signaling downstream of chromosomal instability can exert both anti-tumoral and pro-tumoral effects in a cell-intrinsic manner, suggesting individualized therapy is of great importance. However, further exploration of the cGAS-STING signaling pathway is imperative for the effective management of HNSCC.
First evidence of predation on an adult porbeagle equipped with a pop-off satellite archival tag in the Northwest Atlantic
Authors: Anderson Brooke N. , Horstmyer Lauren , Ballard Kirsty L. , Dodd Jon F. , Sulikowski James A.
Published: September 2024

Pop-off satellite archival tags (PSATs) have been increasingly deployed on marine megafauna to remotely monitor their movements, behavior, and environmental preferences. These tags have also allowed the occasional novel documentation of ecological interactions, such as predation on tagged animals. A PSAT deployed on a pregnant porbeagle Lamna nasus in the Northwest Atlantic suggests the shark was predated upon in mesopelagic waters near Bermuda. Predation was evident approximately 5 months after tagging based on depth and temperature data transmitted by the PSAT. Four days prior to PSAT pop-off, depth data indicated that the tag continued to descend and ascend in the water column while the temperature remained approximately 5°C above ambient levels, even at several hundred meters in depth, indicating ingestion. Given the location of predation and elevated temperature at depth recorded by the pregnant porbeagle’s tag, potential predators include endothermic shark species such as the white shark Carcharodon carcharias and shortfin mako Isurus oxyrhinchus. This is the first evidence of predation on a porbeagle globally and provides novel insight into inter-specific interactions for this large, threatened shark species.
Insights into foraging behavior from multi-day sound recording tags on goose-beaked whales (Ziphius cavirostris) in the Southern California Bight
Authors: Coates Shannon N. , Sweeney David A. , Falcone Erin A. , Watwood Stephanie L. , Rone Brenda K. , DeRuiter Stacy L. , Barlow Jay , Dolan Karin A. , Morrissey Ronald P. , DiMarzio Nancy A. , Jarvis Susan M. , Andrews Russel D. , Schorr Gregory S.
Published: September 2024

Goose-beaked whales (Ziphius cavirostris) are deep-diving cetaceans known for their elusive nature and specialized foraging behavior. In 2019 and 2020, six telemetry tags were deployed on these whales in Southern California, resulting in 395 h of acoustic and diving data. Foraging dives were manually identified by the presence of echolocation pulses and buzzes, and generalized additive models assessed factors influencing foraging behavior. The median bathymetric depth at foraging sites was 1,419 m (IQR = 359), and the maximum dive depth was highly correlated with bathymetry depth. Individuals started echolocating on descent at a median depth of 410 m (IQR = 74); pulses were not observed shallower than 292 m. Echolocation ceased during the bottom phase for 81.6% of dives, at a median depth of 1,265 m (IQR = 472); pulses were not observed shallower than 587 m on ascent. The median depth of buzzes was 1,215 m (IQR = 479) with 63% occurring during the bottom phase. Deeper dives correlated with longer durations of diving and echolocation, greater echolocation end depths, and wider bottom phase echolocation depth inter-quartile range. The median difference between dive depth and bottom phase median echolocation depth was 98.3 m (IQR = 48.5), suggesting whales in this region forage in a narrow band close to the seafloor. In the San Nicolas Basin, individuals exhibited longer echolocation durations, produced more pulses, and started and ended echolocating at greater depths compared to adjacent regions. These records validate and expand upon previous studies, providing insights into factors influencing foraging behavior in an area with high anthropogenic disturbance.
A comprehensive analysis of microbial community differences in four morphologies of mainstream anaerobic ammonia oxidation systems using big-data mining and machine learning
Authors: Zhou Shijie , Zhu Weidi , He Yuhang , Zhang Tianxu , Jiang Zhicheng , Zeng Ming , Wu Nan
Published: September 2024

Achieving carbon neutrality in wastewater treatment plants relies heavily on mainstream anaerobic ammonia oxidation. However, the stability of this process is often compromised, largely due to the significant influence of microbial morphology. This study analyzed 208 microbial samples using bioinformatics and machine learning (ML) across four different morphologies: Suspended Sludge (SS), Biofilm, Granular Sludge (GS) and the Integrated Fixed-film Activated Sludge process (IFAS). The results revealed IFAS’s notably complex and stable community structure, along with the identification of endemic genera and common genera among the four microbial morphologies. Through co-occurrence network analysis, the interaction between microorganisms of various genera was displayed. Utilizing the Extreme Gradient Boosting (XGBoost) model, a ML modeling framework based on microbiome data was developed. The ML-based feature importance analysis identified LD-RB-34 as a key organism in SS and BSV26 was an important bacterium in IFAS. Additionally, functional bacteria KF-JG30-C25 occupied a higher proportion in GS, and Unclassified Brocadiaceae occupied a higher proportion in Biofilm. Furthermore, dissolved oxygen, temperature and pH were identified as the primary factors determining microbial communities and influencing anammox activity. Overall, this study deepens our understanding of bacterial communities to enhance the mainstream anammox nitrogen removal.
Risk factors for regional lymph node metastasis in rectal neuroendocrine tumors: a population-based study
Authors: Li Ruizhen , Li Xiaofen , Wang Yan , Chang Chen , Lv Wanrui , Li Xiaoying , Cao Dan
Published: September 2024

Introduction

The identification of risk factors for regional lymph node (r-LN) metastasis in rectal neuroendocrine tumors (R-NETs) remains challenging. Our objective was to investigate the risk factors associated with patients diagnosed with R-NETs exhibiting r-LN metastasis.

Methods

Patient information was obtained from the Surveillance, Epidemiology, and End Results (SEER) database, complemented by data from the West China Hospital (WCH) databases. The construction cohort comprised patients diagnosed with R-NETs from the SEER database, while cases from the WCH database were utilized as the validation cohort. A novel nomogram was developed to predict the probability of r-LN metastasis, employing a logistic regression model.

Results

Univariate analysis identified four independent risk factors associated with poor r-LN metastasis: age (HR = 1.027, p < 0.05), grade (HR = 0.010, p < 0.05), T stage (HR = 0.010, p < 0.05), and tumor size (HR = 0.005, p < 0.05). These factors were selected as predictors for nomogram construction.

Discussion

The novel nomogram serves as a reliable tool for predicting the risk of r-LN metastasis, providing clinicians with valuable assistance in identifying high-risk patients and tailoring individualized treatments.
The mechanics of the retina: Müller glia role on retinal extracellular matrix and modelling
Authors: Prieto-López Laura , Pereiro Xandra , Vecino Elena
Published: September 2024

The retina is a highly heterogeneous tissue, both cell-wise but also regarding its extracellular matrix (ECM). The stiffness of the ECM is pivotal in retinal development and maturation and has also been associated with the onset and/or progression of numerous retinal pathologies, such as glaucoma, proliferative vitreoretinopathy (PVR), age-related macular degeneration (AMD), epiretinal membrane (ERM) formation or uveitis. Nonetheless, much remains unknown about the biomechanical milieu of the retina, and specifically the role that Müller glia play as principal mechanosensors and major producers of ECM constituents. So far, new approaches need to be developed to further the knowledge in the field of retinal mechanobiology for ECM-target applications to arise. In this review, we focus on the involvement of Müller glia in shaping and altering the retinal ECM under both physiological and pathological conditions and look into various biomaterial options to more accurately replicate the impact of matrix stiffness in vitro.
The impact of pulmonary tuberculosis on SARS-CoV-2 infection: a nationwide cohort study
Authors: Lee Sang Hwan , Kim Yun Jin , Oh Jaehoon , Kang Hyunggoo , Yoo Kyung Hun , Ko Byuk Sung , Lim Tae Ho , Kim Bo-Guen , Lee Hyun , Kim Sang-Heon , Sohn Jang Won , Yoon Ho Joo , Choi Hayoung , Cho Yongil , Park Dong Won
Published: September 2024

Background

The interaction between COVID-19 and tuberculosis (TB) is not yet fully understood, and large-scale research on the mortality outcome of such dual infection has been limited. This study aimed to investigate the impact of PTB on mortality among patients with COVID-19 within a Korean population by conducting an extensive analysis of a nationwide large dataset.

Method

We investigated the mortality and disease severity among COVID-19 patients who had PTB in South Korea. This study analyzed 462,444 out of 566,494 COVID-19 patients identified between January 2020 and December 2021.

Result

A total of 203 COVID-19 with PTB patients and 812 matched COVID-19 without PTB were analyzed using 1:4 propensity score matching. COVID-19 patients with PTB exhibited higher in-hospital mortality (odds ratio (OR) 3.02, 95% confidence interval (CI) 1.45–6.27, p-value = 0.003) and were at increased risk of requiring conventional oxygen therapy (OR 1.57, 95% CI 1.10–2.25, p-value = 0.013) as well as high flow nasal cannula (HFNC) or noninvasive ventilation (NIV) oxygen therapy (OR 1.91, 95 CI 1.10–3.32, p-value = 0.022) compared to those without PTB. Compared to matched COVID-19 without PTB, co-infected patients showed increased mortality rates across various timeframes, including during hospitalization, and at 30 day and 90 day intervals. In-hospital mortality rates were particularly elevated among women, individuals with malignancy, and those with lower incomes. Furthermore, the increased in-hospital mortality among PTB patients persisted irrespective of the timing of TB diagnosis or vaccination status against COVID-19.

Conclusion

We suggest that physicians be aware of the risk of mortality and severity among COVID-19 patients with PTB; coinfection with COVID-19 is a critical situation that remains to be further explored and needs more attention in countries with an intermediate to high PTB burden.
Correlation between ratio of fasting blood glucose to high density lipoprotein cholesterol in serum and non-alcoholic fatty liver disease in American adults: a population based analysis
Authors: Jin Xianjing , Xu Jing , Weng Xiaochun
Published: September 2024

Background

Based on previous research, elevated fasting blood glucose (FBG) and decreased high-density lipoprotein cholesterol (HDL-C) levels are associated with non-alcoholic fatty liver disease (NAFLD). It is hypothesized that the prevalence of NAFLD may be proportional to the FBG-to-HDL-C ratio (GHR).

Methods

In this study, 3,842 participants from the National Health and Nutrition Examination Survey (NHANES) (2013–2020) were investigated. Liver steatosis was assessed using vibration-controlled transient elastography (VCTE). NAFLD was defined as controlled attenuation parameter (CAP) ≥288 dB/m.

Results

After adjusting for race, gender, age, diabetes, BMI, moderate activities, uric acid, albumin, ALT, GGT, ALP, total bilirubin and creatinine, multiple logistic regression analysis indicated a positive correlation between GHR and the prevalence of NAFLD (OR = 1.22, 95% CI = 1.17–1.28). Additionally, multiple linear regression analysis showed a positive correlation between GHR and the severity of liver steatosis according to CA p-values (β = 4.97, 95% CI: 4.28, 5.66). According to the subgroup analysis, the correlation was stronger in other race, participants at the age <50 years old and those with non-diabetes. In this study, a non-linear relationship and saturation effect between GHR and the prevalence of NAFLD was also revealed, characterized by an inverted L-shaped curve, with an inflection point of 7.443. Finally, the receiver operating characteristic (ROC) analysis suggested that the area under the curve (AUC) of GHR (AUC = 0.731) significantly exceeded that of FBG and HDL-C.

Conclusion

Elevated GHR levels are independently associated with the severity of liver steatosis and the increased prevalence of NAFLD in American adults.
A combination analysis based on bioinformatics tools reveals new signature genes related to maternal obesity and fetal programming
Authors: Liu Chunhong , Lu Yulan , Huang Chunchuan , Zeng Yonglong , Zheng Yuye , Wang Chunfang , Huang Huatuo
Published: September 2024

Background

Maternal obesity significantly influences fetal development and health later in life; however, the molecular mechanisms behind it remain unclear. This study aims to investigate signature genes related to maternal obesity and fetal programming based on a genomic-wide transcriptional placental study using a combination of different bioinformatics tools.

Methods

The dataset (GSE128381) was obtained from Gene Expression Omnibus (GEO). The data of 100 normal body mass index (BMI) and 27 obese mothers were included in the analysis. Differentially expressed genes (DEGs) were evaluated by limma package. Thereafter, functional enrichment analysis was implemented. Then, weighted gene co-expression network analysis (WGCNA) and the least absolute shrinkage and selection operator (LASSO) analysis were used to further screening of signature genes. Simple linear regression analysis was used to assess the correlation between signature genes and newborn birth weight. Gene set enrichment analysis (GSEA) was implemented to study signaling pathways related to signature genes. The expression of the signature genes was also explored in 48 overweight mothers in the same dataset.

Results

A total of 167 DEGs were obtained, of which 122 were up-regulated while 45 were down-regulated. The dataset was then clustered into 11 modules by WGCNA, and the MEbrown was found as the most significant module related to maternal obesity and fetal programming (cor = 0.2, p = 0.03). The LASSO analysis showed that PTX3, NCF2, HOXB5, ABCA6, and C1orf162 are signature genes related to maternal obesity and fetal programming, which were increased in the placenta of obese mothers compared to those with normal BMI. The area under the curve (AUC) of the signature genes in the receiver operating characteristic curve (ROC) was 0.709, 0.660, 0.674, 0.667, and 0.717, respectively. Simple linear regression analysis showed that HOXB5 was associated with newborn birth weight. GSEA analysis revealed that these signature genes positively participate in various signaling pathways/functions in the placenta.

Conclusion

PTX3, NCF2, HOXB5, ABCA6, and C1orf162 are novel signature genes related to maternal obesity and fetal programming, of which HOXB5 is implicated in newborn birth weight.
SARS-CoV-2 replicates in the placenta after maternal infection during pregnancy
Authors: Radan Anda-Petronela , Renz Patricia , Raio Luigi , Villiger Anna-Sophie , Haesler Valérie , Trippel Mafalda , Surbek Daniel
Published: September 2024

Objectives

Pregnant women are at increased risk for severe SARS-CoV-2 infection and adverse neonatal outcome, primarily preterm birth and stillbirth. Our study aimed to investigate to which extent SARS-CoV-2 affects placental tissue and if viral replication within the placenta is evident, thus if there is a correlation between placental damage and adverse pregnancy outcome such as stillbirth.

Methods

We prospectively collected placentas from 61 SARS-CoV-2 infected pregnant women and 10 controls. Histopathological, immunohistochemical, and in situ hybridization studies were performed on all placentas with antibodies for SARS-CoV-2 proteins, ACE2, various immune cells, and inflammatory markers or probes for SARS-CoV-2 genes and an antisense strand.

Results

The measured scores of SARS-CoV-2 glycoprotein, nucleocapsid, and antisense strand indicating replication correlated with both the severity of maternal symptoms and presence of stillbirth. Specifically, 15/61 placentas exhibited replication, while the three cases with stillbirth had high or maximal replication scores. ACE2-H-score was significantly higher in COVID-19 patients, while the expression of various immune cells did not differ statistically. In multivariate analysis, presence of maternal comorbidities correlated with presence of severe COVID-19 infection.

Conclusion

We report evidence of active in vivo SARS-CoV-2 replication in the placenta after maternal infection in pregnancy in a case–control setting in a large population. Intensity of placental viral replication as well as viral levels were higher in women with severe or critical COVID-19 disease, supporting the rationale that severity of maternal SARS-CoV-2 infection could correlate with the severity of placentitis. Replication was maximal in cases of stillbirth, which suggests direct placental involvement in the pathophysiology of this dramatic outcome. Continuing to advocate for preventive measures against COVID-19 during pregnancy, including (re)vaccination, as well as appropriately counseling women with diagnosed infection, are of utter importance.
Development of a suite of short planetary health learning resources by students for students as future health professionals
Authors: Pais Rodrigues Catarina , Papageorgiou Evangelos , McLean Michelle
Published: September 2024

Planetary health recognizes the interdependencies between human health and the well-being of the Earth’s ecosystems. Human activities have led to the disruption and transformation of natural systems and a range of global environmental changes such as climate change, air pollution, and biodiversity loss. Health professionals must be equipped to deal with the health impacts of global environmental changes. This article describes the development and usage of a suite of 14 short online learning resources (‘bricks’) on Planetary Health on the ScholarRx platform. There are several principles that inform the development of these bricks, including learner-centric, peer learning, diversity, equity and inclusion, and authentic learning. The content is developed using a student-educator collaboration model, supported by an editorial team. The suite of 14 modules was published in June 2023, with the initial usage data promising with 1,990 views in the first 10 months. These digital, modular resources allow for easy dissemination and can be incorporated in different programs depending on context and need.
Association of EPA and DHA with age-related macular degeneration: a cross-sectional study from NHANES
Authors: Li Kewei , Liu Jialing , Li Xuhui , Liu Xiaozhu , Hu Pengcheng , He Ming
Published: September 2024

Purpose

This cross-sectional study conducted in the general US population investigated the association between dietary intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and the prevalence of AMD.

Methods

Data from the National Health and Nutrition Examination Survey (NHANES) were utilized, including 4,842 participants aged 40 years and older. Dietary EPA and DHA intake data were collected through two 24-h dietary recall interviews and adjusted for weight. AMD was determined by a standardized grading system based on the presence of key features of AMD in color photographs of the macula. Multivariate logistic regression and restricted cubic spline models evaluated the associations between dietary EPA and DHA intake and AMD. Subgroup analysis and interaction analysis explored the influence of covariates.

Results

A total of 4,842 participants were included. In the multivariate-adjusted model 2, the odds ratios (ORs) with 95% confidence intervals (CIs) for AMD were 0.86 (0.75, 0.99) and 0.88 (0.80, 0.97) per unit increase in dietary EPA and DHA intake, respectively. Interaction testing revealed significant effect modification by age, education, and BMI on the EPA-AMD association, indicating these factors significantly impacted this inverse relationship (p-interaction < 0.05). Similarly, age, education, BMI, and cataract surgery history modified the inverse DHA-AMD association (p-interaction < 0.05). Dose-response analyses demonstrated a negative correlation between dietary EPA and DHA intake with AMD prevalence (p-nonlinearity = 0.184 and 0.548, respectively).

Conclusion

Our findings suggested that higher dietary EPA and DHA intake could be associated with lower AMD risk in older US adults. Age, education level, BMI, and history of cataract surgery may influence this inverse association.
Association between the systemic inflammation response index and mortality in the asthma population
Authors: Xu Feng , Jiang Hui , Li Fanglan , Wen Yan , Jiang Pan , Chen Feng , Feng Yongwen
Published: September 2024

Background

As a novel indicator of inflammation, the relationship between the systemic immune-inflammation index (SIRI) and mortality in patients with asthma remains uncertain. Our study aimed to explore the association between SIRI and mortality in asthma patients.

Methods

Data from the National Health and Nutrition Examination Survey (NHANES) for US adults from 2001 to 2018 were included in this study. Then, we divided all patients into three groups based on SIRI tertiles and used multivariable weighted Cox regression analysis, smoothing curve fitting, survival curve analysis, and subgroup analysis to investigate the relationship between SIRI and asthma.

Results

A total of 6,156 participants were included in the study, with each SIRI tertile consisting of 2052 individuals. Asthma patients with higher SIRI levels were older, had a higher level of education, were more likely to be married, and had a higher chance of being smokers. In Cox proportional-hazards models, the highest SIRI group showed higher hazard ratios (HRs) for all-cause mortality in individuals with asthma after adjusting for potential confounders. The restricted cubic spline analysis indicated a non-linear relationship between SIRI and all-cause mortality. The Kaplan–Meier survival curves showed that patients with higher SIRI levels had a higher risk of all-cause mortality. Subgroup analyses revealed SIRI’s association with all-cause mortality across various demographics, including age, sex, race, education levels, smoking status, and marital status.

Conclusion

Our study provides evidence for the relationship between SIRI and mortality in asthma patients. SIRI may potentially serve as a predictive tool for evaluating asthma mortality rates.
Effects of developmental stages, sex difference, and diet types of the host marmalade hoverfly (Episyrphus balteatus) on symbiotic bacteria
Authors: Wang Xiaoyun , Huangfu Ningbo , Chen Lulu , Zhang Kaixin , Li Dongyang , Gao Xueke , Li Bingbing , Wang Li , Zhu Xiangzhen , Ji Jichao , Luo Junyu , Cui Jinjie
Published: September 2024

Introduction

Symbiotic bacteria play key roles in a variety of important life processes of insects such as development, reproduction and environmental adaptation, and the elucidation of symbiont population structure and dynamics is crucial for revealing the underlying regulatory mechanisms. The marmalade hoverfly (Episyrphus balteatus) is not only a remarkable aphid predator, but also a worldwide pollinator second to honeybees. However, its symbiont composition and dynamics remain unclear.

Methods

Herein, we investigate the symbiotic bacterial dynamics in marmalade hoverfly throughout whole life cycle, across two sexes, and in its prey Megoura crassicauda by 16S rRNA sequencing.

Results

In general, the dominant phyla were Proteobacteria and Firmicutes, and the dominant genera were Serratia and Wolbachia. Serratia mainly existed in the larval stage of hoverfly with the highest relative abundance of 86.24% in the 1st instar larvae. Wolbachia was found in adults and eggs with the highest relative abundance of 62.80% in eggs. Significant difference in species diversity was observed between the adults feeding on pollen and larvae feeding on M. crassicauda, in which the dominant symbiotic bacteria were Asaia and Serratia, respectively. However, between two sexes, the symbionts exhibited high similarity in species composition. In addition, our results suggested that E. balteatus obtainded Serratia mainly through horizontal transmission by feeding on prey aphids, whereas it acquired Wolbachia mainly through intergeneration vertical transmission. Taken together, our study revealed the effects of development stages, diet types and genders of E. balteatus on symbionts, and explored transmission modes of dominant bacteria Serratia and Wolbachia.

Discussion

Our findings lay a foundation for further studying the roles of symbiotic bacteria in E. balteatus life cycle, which will benefit for revealing the co-adaptation mechanisms of insects and symbiotic bacteria.
Geographic and environmental impacts on gut microbiome in Himalayan langurs (Semnopithecus schistaceus) and Xizang macaques (Macaca mulatta vestita)
Authors: Wang Xueyu , Li Hong , Yang Yumin , Wu Zhijiu , Wang Zhixiang , Li Dayong , Xia Wancai , Zou Shuzhen , Liu Yujia , Wang Fan
Published: September 2024

Introduction

Gut microbiome plays a crucial role in the health of wild animals. Their structural and functional properties not only reflect the host’s dietary habits and habitat conditions but also provide essential support for ecological adaptation in various environments.

Methods

This study investigated the gut microbiome of Himalayan langurs (Semnopithecus schistaceus) and Xizang macaques (Macaca mulatta vestita) across different geographic regions using 16S rRNA gene and metagenomic sequencing.

Results

Results showed distinct clustering patterns in gut microbiota based on geographic location. Soil had an insignificant impact on host gut microbiome. Himalayan langurs from mid-altitude regions exhibited higher levels of antibiotic resistance genes associated with multidrug resistance, while Xizang macaques from high-altitude regions showed a broader range of resistance genes. Variations in carbohydrate-active enzymes and KEGG pathways indicated unique metabolic adaptations to different environments.

Discussion

These findings provide valuable insights into the health and conservation of these primates and the broader implications of microbial ecology and functional adaptations in extreme conditions.
Spatial differentiation and influencing factors of effective phosphorus in cultivated soil in the water source area of the mid-route of South-to-North water transfer project
Authors: Wu Zhengxiang , Zhou Yang , Wang Miao
Published: September 2024

The long-term application of phosphate fertilizers in agricultural production leads to a large accumulation of phosphorus in the soil. When it exceeds a certain limit, phosphorus will migrate to surrounding water bodies through surface runoff and other mechanisms, potentially causing environmental risks such as eutrophication of water bodies and increasing the risk of water source pollution. This study takes Shiyan City, the water resources area of the mid-route of the South-to-North Diversion Project (MSDP), as the study area. Based on 701 sampling points of topsoil, geostatistics and geodetectors were used to explore the spatial heterogeneity and influencing factors of available phosphorus (AP) in the topsoil of the area. The results show that the effective phosphorus content in the topsoil of the study area ranges from 0.30 to 146.00 mg/kg, with an average value of 14.28 mg/kg, showing strong variability characteristics. Geostatistical analysis shows that among all theoretical models, the exponential model has the best fitting effect, with a lump gold effect of 0.447 and a range of 82,000 m. The soil available phosphorus content shows an increasing trend from the Central Valley lowlands to the surrounding mountainous hills. Among them, elevation is the main controlling factor for the spatial variation of available phosphorus in the topsoil, followed by soil types, planting systems, annual precipitation, and organic matter. The non-linear enhancement or dual-factor enhancement among various environmental factors reveals the diversity and complexity of spatial heterogeneity affecting available phosphorus content in cultivated soil. This study could provide scientific references for maintaining ecological security in the water source area of the MSDP, improving the precise management of AP, and enhancing cultivated land quality.
A comprehensive review of influenza B virus, its biological and clinical aspects
Authors: Ashraf Muhammad Awais , Raza Muhammad Asif , Amjad Muhammad Nabeel , ud Din Ghayyas , Yue Lihuan , Shen Bei , Chen Lingdie , Dong Wei , Xu Huiting , Hu Yihong
Published: September 2024

Influenza B virus (IBV) stands as a paradox, often overshadowed by its more notorious counterpart, influenza A virus (IAV). Yet, it remains a captivating and elusive subject of scientific inquiry. Influenza B is important because it causes seasonal flu outbreaks that can lead to severe respiratory illnesses, including bronchitis, pneumonia, and exacerbations of chronic conditions like asthma. Limitations in the influenza B virus’s epidemiological, immunological, and etiological evolution must be addressed promptly. This comprehensive review covers evolutionary epidemiology and pathogenesis, host-virus interactions, viral isolation and propagation, advanced molecular detection assays, vaccine composition and no animal reservoir for influenza B virus. Complex viral etiology begins with intranasal transmission of influenza B virus with the release of a segmented RNA genome that attacks host cell machinery for transcription and translation within the nucleus and the release of viral progeny. Influenza B virus prevalence in domesticated and wild canines, sea mammals, and birds is frequent, yet there is no zoonosis. The periodic circulation of influenza B virus indicates a 1–3-year cycle for monophyletic strain replacement within the Victoria strain due to frequent antigenic drift in the HA near the receptor-binding site (RBS), while the antigenic stability of Yamagata viruses portrays a more conservative evolutionary pattern. Additionally, this article outlines contemporary antiviral strategies, including pharmacological interventions and vaccination efforts. This article serves as a resource for researchers, healthcare professionals, and anyone interested in the mysterious nature of the influenza B virus. It provides valuable insights and knowledge essential for comprehending and effectively countering this viral foe, which continues to pose a significant public health threat.