bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhanced axial resolution of lattice light sheet microscopy by fluorescence differential detection
Authors: Gan Yanhong , Ma Ye , Gong Wenwen , Liu Wenjie , Wang Ziang , Hao Xiang , Han Yubing , Kuang Cuifnag , Liu Xu
Published: July 2022

Lattice light-sheet microscopy (LLSM) is promising in long-term biological volumetric imaging due to its high spatiotemporal resolution and low phototoxicity. However, three-dimensional (3D) isotropic spatial resolution remains an unmet goal in LLSM because of its poorer axial resolution. Combing LLSM with fluorescence differential detection, namely LLSDM, has been proposed to improve the axial resolution of LLSM in simulation. It demonstrates the possibility of further enhancing the axial resolution in 3D volumetric imaging with LLSM by specifically discarding the off-focus photons captured using a complementary optical lattice (OL) profile generated with additional 0-π phase modulation at the objective pupil plane. The direct generation of the complementary lattice profile using the binary phase modulator conjugated to the sample plane for amplitude modulation, as used in LLSM, is also permittable. Nevertheless, the previously proposed configuration fails to provide a symmetric complementary lattice pattern along the axial axis, thus leading to the imbalanced off-focus photon suppression in the reconstructed images after subtraction [Opt. Lett. 45, 2854 (2020)10.1364/OL.393378]. Here, we modified the LLSDM theory which can produce an ideal complementary lattice pattern with central zero intensity and symmetrically distributed sidelobes. We also analyzed the impact of numerical aperture matching between the original and complementary lattice patterns and presented the consistency between the simulated and experimental results. As demonstrated by imaging the distribution of fluorescent beads and microtubules in fixed U2OS cells, as well as the dynamics of filopodia in live U2OS cells, LLSDM provides about 1.5 times improvement in axial resolution, and higher imaging contrast compared with traditional LLSM.
Machine learning-assisted design of polarization-controlled dynamically switchable full-color metasurfaces
Authors: Hu Lechuan , Ma Lanxin , Wang Chengchao , Liu Linhua
Published: July 2022

Dynamic color tuning has significant application prospects in the fields of color display, steganography, and information encryption. However, most methods for color switching require external stimuli, which increases the structural complexity and hinders the applicability of front-end dynamic display technology. In this study, we propose polarization-controlled hybrid metal-dielectric metasurfaces to realize full-color display and dynamic color tuning by altering the polarization angle of incident light without changing the structure and properties of the material. A bidirectional neural network is trained to predict the colors of mixed metasurfaces and inversely design the geometric parameters for the desired colors, which is less dependent on design experience and reduces the computational cost. According to the color recognition ability of human eyes, the accuracy of color prediction realized in our study is 93.18% and that of inverse parameter design is 92.37%. This study presents a simple method for dynamic structural color tuning and accelerating the design of full-color metasurfaces, which can offer further insight into the design of color filters and promote photonics research.
Flexible passive integrated photonic devices with superior optical and mechanical performance
Authors: Luo Ye , Sun Chunlei , Ma Hui , Wei Maoliang , Li Junying , Jian Jialing , Zhong Chuyu , Chen Zequn , Tang Renjie , Richardson Kathleen A. , Lin Hongtao , Li Lan
Published: July 2022

Flexible integrated photonics is a rapidly emerging technology with a wide range of possible applications in the fields of flexible optical interconnects, conformal multiplexing sensing, health monitoring, and biotechnology. One major challenge in developing mechanically flexible integrated photonics is the functional component within an integrated photonic circuit with superior performance. In this work, several essential flexible passive devices for such a circuit were designed and fabricated based on a multi-neutral-axis mechanical design and a monolithic integration technique. The propagation loss of the waveguide is calculated to be 4.2 dB/cm. In addition, we demonstrate a microring resonator, waveguide crossing, multimode interferometer (MMI), and Mach–Zehnder interferometer (MZI) for use at 1.55 µm, each exhibiting superior optical and mechanical performance. These results represent a significant step towards further exploring a complete flexible photonic integrated circuit.
Assessment of spectral properties of positive dysphotopsia or glare caused by LED sources for different types of intraocular lenses
Authors: Das Kamal K. , Kalapala Akhil R.
Published: July 2022

Light emitting diodes (LEDs) have become a major source of lighting conditions. The increased prevalence of LED light sources introduces new concerns for the spectral effects of positive dysphotopsia (PD) or glare type photic phenomena for pseudo-phakic patients with intraocular lenses (IOLs). A significant amount of work has been published in the area of spectral discomfort and sensitivity of LEDs as well as automotive lighting. The wavelength dependence or spectral properties of PD due to LEDs for IOLs has not been reported. This study, to our knowledge, is the first one to assess the glare characteristics of four commercially available IOL models with different material types and design features using an optical bench and non-sequential ray trace simulations with LEDs of different wavelengths. A novel approach of representing the reflected and transmitted IOL glare utilizing Fresnel coefficients is found to be in close agreement with the measurements.
Achieving sub-nanometer roughness on aspheric optical mold by non-contact polishing using damping-clothed tool
Authors: Zhang Pengfei , Li Linguang , Yang Zhe , Pan Bo , Zhang Meng , Guo Xiaoguang , Li Gang , Kim Daewook , Guo Jiang
Published: July 2022

The surface quality of optical lenses is highly required in imaging functions. Normally, ultra-precision turning is employed to fabricate the optical lenses. However, ultra-precision turning cannot meet the surface quality demands due to the tool marks. In this study, a new damping-clothed (DC) tool and chemical enhanced non-Newtonian ultrafine (CNNU) slurry for non-contact polishing are proposed to achieve sub-nanometer roughness on aspherical optical molds. A material removal model based on the hydrodynamic pressure and velocity simulation was established to calculate the dwell time in curved surface machining. The formation mechanism of sub-nanometer roughness is clarified. The proposed method and slurry were verified by the experiments in processing NiP alloy aspheric optical mold. After the process, surface roughness Sa achieved 0.54 nm and the form accuracy is less than PV 600 nm.
Lidar backscatter simulation for angular scanning of cirrus clouds with quasi-horizontally oriented ice crystals
Authors: Kustova Natalia , Konoshonkin Alexander , Kokhanenko Grigorii , Wang Zhenzhu , Shishko Victor , Timofeev Dmitriy , Borovoi Anatoli
Published: July 2022

Backscattering properties of ice crystals are numerically investigated in the case of plate-like quasi-horizontally oriented crystals of cirrus clouds. In this case, a vertically oriented lidar detects the specular reflection from the clouds while a lidar with angular scanning allows one to infer the microphysical properties like the transverse shape of the crystals. It is shown that the depolarization ratio as a function of the lidar tilt reveals a step at a lidar tilt of about 30° from the vertical. This step has been observed experimentally. Appearance of this step indicates that the transverse shapes of the plate-like crystals are regular.
Performance enhancement of nitrogen-polar GaN-based light-emitting diodes prepared by metalorganic chemical vapor deposition
Authors: Wang Yang , Wang Yusen , Zhang Lidong , Niu Yunfei , Yu Jiaqi , Ma Haotian , Lu Chao , Shi Zhifeng , Deng Gaoqiang , Zhang Baolin , Zhang Yuantao
Published: July 2022

Nitrogen-polar (N-polar) III-nitride materials have great potential for application in long-wavelength light-emitting diodes (LEDs). However, the poor quality of N-polar nitride materials hinders the development of N-polar devices. In this work, we report the enhanced performance of N-polar GaN-based LEDs with an optimized InGaN/GaN double quantum well (DQW) structure grown by metalorganic chemical vapor deposition. We improved the quality of the N-polar InGaN/GaN DQWs by elevating the growth temperature and introducing hydrogen as the carrier gas during the growth of the quantum barrier layers. N-polar LEDs prepared based on the optimized InGaN/GaN DQWs show significantly enhanced (by over 90%) external quantum efficiency and a weakened droop effect compared with a reference LED. More importantly, the optimized N-polar DQWs show a significantly longer emission wavelength than Ga-polar DQWs grown at the same QW growth temperature. This work provides a feasible approach to improving the quality of the N-polar InGaN/GaN QW structure, and it will promote the development of N-polar GaN-based long-wavelength light-emitting devices for micro-LED displays.
Non-planar illumination deflectometry for axicon metrology
Authors: Quach Henry , Kang Hyukmo , Jeong Byeongjoon , Choi Heejoo , Kim Daewook
Published: July 2022

We introduce an on-axis deflectometry test configuration for axicon metrology. Axicons are challenging to measure due to their characteristically steep, convex geometry. However, if an axicon is coaxially aligned with a camera and a surrounding cylindrical illumination source, high-resolution surface measurements can be obtained via the principle of deflectometry. Emitted from the temporally modulated source, light deflects at the conical surface and into the entrance pupil of a camera, illuminating the full axicon aperture except the

ø

0.5-mm rounded tip. Deflectometry measurements of a 100° and 140° axicon show holistic cone angle agreement within 0.035° against touch probe data and up to 7.93 root

μ

m mean square difference from a best-fit cone. We discuss the non-planar illumination architecture, sensitivity, and experimental results of arbitrary apex angle axicons.
Simultaneous measurement of displacement and temperature using a balloon-like hybrid fiber sensor
Authors: Santos João P. , Bierlich Jörg , Kobelke Jens , Ferreira Marta S.
Published: July 2022

A fiber sensor based on a silica capillary in a balloon-like shape for simultaneous measurement of displacement and temperature is proposed and experimentally demonstrated. The sensor is fabricated by splicing a segment of a hollow-core fiber between two single-mode fibers (SMF) and by creating a balloon shape with the capillary at the top-center position. The SMF–capillary–SMF configuration excites an antiresonant (AR) guidance, and the balloon shape enhances the Mach–Zehnder interferometer (MZI). Experimental results show that, for a balloon length of 4.0 cm and a capillary length of 1.2 cm, the AR is insensitive to displacement and its sensitivity to temperature is 14.3 pm/°C, while the MZI has a sensitivity to displacement of 1.68 nm/mm in the range between 0 and 5 mm and a sensitivity to temperature of 28.6 pm/°C, twice the value of the AR. The proposed fiber sensor has only one sensing element in one configuration, which makes it simple to fabricate as well as low cost.
Polarization multiplexed dissipative Kerr solitons in an on-chip micro-resonator
Authors: Geng Yong , Xiao Yanlan , Han Xinjie , Qiu Kun , Xu Jing , Zhou Heng
Published: July 2022

We demonstrate polarization multiplexed dissipative Kerr solitons in an on-chip silicon nitride micro-resonator. In our experiment, TE- and TM-polarized solitons can be individually generated and controlled, thanks to their weak mutual interaction as the result of sufficiently different repetition rates and orthogonal polarization states. Furthermore, we find that TE- and TM-polarized solitons usually exhibit uncorrelated time jitters. Therefore, the frequency and phase coherence between the polarization multiplexed soliton microcombs change dramatically as a function of pump laser parameters, where by optimization, we achieve a narrow dual-microcomb beat note linewidth as small as 4.4 kHz. Potential applications of on-chip polarization multiplexed soliton microcombs include Kerr comb spectral expansion, dual-comb metrology, and measurement of quantum entanglements.
Femtosecond-laser-enabled simultaneous figuring and finishing of glass with a subnanometer optical surface
Authors: Chen Gong , Qiao Jie
Published: July 2022

We demonstrate simultaneous figuring and surface finishing of glass using a femtosecond laser. For the first time, to the best of our knowledge, we have achieved deterministic material removal with nanometer precision and maintained sub-nanometer surface roughness without inducing any mid-spatial-frequency errors to the initial surface. A dynamic pulse propagation model is established to predict the interaction process, including plasma generation and surface temperature. The interactive modeling and the experiments enable the selection of a set of laser parameters to achieve controllable optical figuring and finishing. This demonstration shows the potential for using femtosecond lasers for advanced freeform optic forming, finishing, and reduction of detrimental mid-spatial-frequency errors, and laser-ablation-based patterning used for fabrication of integrated photonics and lasers.
Histologically resolved multiomics enables precise molecular profiling of human intratumor heterogeneity
Authors: Chen Tao , Cao Chen , Zhang Jianyun , Streets Aaron , Li Tiejun , Huang Yanyi
Published: July 2022

Both the composition of cell types and their spatial distribution in a tissue play a critical role in cellular function, organ development, and disease progression. For example, intratumor heterogeneity and the distribution of transcriptional and genetic events in single cells drive the genesis and development of cancer. However, it can be challenging to fully characterize the molecular profile of cells in a tissue with high spatial resolution because microscopy has limited ability to extract comprehensive genomic information, and the spatial resolution of genomic techniques tends to be limited by dissection. There is a growing need for tools that can be used to explore the relationship between histological features, gene expression patterns, and spatially correlated genomic alterations in healthy and diseased tissue samples. Here, we present a technique that combines label-free histology with spatially resolved multiomics in unfixed and unstained tissue sections. This approach leverages stimulated Raman scattering microscopy to provide chemical contrast that reveals histological tissue architecture, allowing for high-resolution in situ laser microdissection of regions of interests. These microtissue samples are then processed for DNA and RNA sequencing to identify unique genetic profiles that correspond to distinct anatomical regions. We demonstrate the capabilities of this technique by mapping gene expression and copy number alterations to histologically defined regions in human oral squamous cell carcinoma (OSCC). Our approach provides complementary insights in tumorigenesis and offers an integrative tool for macroscale cancer tissues with spatial multiomics assessments.
“We face the same risk as the other health workers”: Perceptions and experiences of community pharmacists in Indonesia during the COVID-19 pandemic
Authors: Wulandari Luh Putu Lila , Khan Mishal , Probandari Ari , Batura Neha , Ferdiana Astri , Mashuri Yusuf Ari , Wibawa Tri , Daraninggar Dea , Dewi Berlian Kusuma , Day Ric , Jan Stephen , Schierhout Gill , Yeung Shunmay , Wiseman Virginia , Liverani Marco
Published: July 2022

In many countries, community pharmacies have played an important role during the COVID-19 pandemic, providing essential medicines and personal protective equipment (PPE), disseminating information on disease prevention and management, and referring clients to health facilities. In recognition of this, there are increasing calls for an improved understanding of the challenges and experiences faced by these providers during the COVID-19 pandemic, with a view to providing them with better support and guidance now and during future emergencies. Between January and February 2021 we conducted 21 qualitative interviews to explore the experiences, safety concerns, and attitudes of pharmacists and pharmacy technicians during the COVID-19 crisis in Indonesia, a country that has recorded more than four million cases since the start of the pandemic. Interview transcripts were analysed using thematic content analysis. Findings indicate that COVID-19 has had a significant impact on pharmacy practices in Indonesia. Most participants implemented preventive measures and adapted their business models to the changing circumstances. The shift to remote sales and home delivery allowed many pharmacies to maintain, and even increase their profit margins due to greater demand for medicines and PPE. However, many participants were concerned about the increased risk of infection due to limited social distancing and prolonged interactions with clients, many of whom displayed COVID-19 symptoms. Importantly, there was a general perception that the government did not sufficiently recognize these risks. In conclusion, the government should consider developing additional operational guidelines and regulatory frameworks to improve the safety, operation, and involvement of community pharmacies in the current pandemic response efforts and any future public health emergencies.
Partial masculinization of Aedes aegypti females by conditional expression of Nix
Authors: Kojin Bianca B. , Jakes Emma , Biedler James K. , Tu Zhijian , Adelman Zach N.
Published: July 2022

Background

Aedes aegypti, the main vector of dengue, yellow fever, and other arboviruses thrives in tropical and subtropical areas around the globe putting half of the world’s population at risk. Despite aggressive efforts to control the transmission of those viruses, an unacceptable number of cases occur every year, emphasizing the need to develop new control strategies. Proposals for vector control focused on population suppression could offer a feasible alternative method to reduce disease transmission. The induction of extreme male-biased sex ratios has been hypothesized to be able to suppress or collapse a population, with previous experiments showing that stable expression of the male determining factor Nix in A. aegypti is sufficient to convert females into fertile males.

Methodology/Principal findings

Here, we report on the conditional expression of Nix in transgenic A. aegypti under the control of the tetracycline-dependent (Tet-off) system, with the goal of establishing repressible sex distortion. A masculinization phenotype was observed in three of the seven transgenic lines with females exhibiting male-like long maxillary palps and most importantly, the masculinized females were unable to blood feed. Doxycycline treatment of the transgenic lines only partially restored the normal phenotype from the masculinized transgenic lines, while RT-qPCR analysis of early embryos or adults showed no correlation between the level of masculinization and ectopic Nix expression.

Conclusions/Significance

While the conditional expression of Nix produced intersex phenotypes, the level of expression was insufficient to program full conversion. Modifications that increase both the level of activation (no tet) and the level of repression (with tet) will be necessary, as such this study represents one step forward in the development of genetic strategies to control vector-borne diseases via sex ratio distortion.
The impact of monosomies, trisomies and segmental aneuploidies on chromosomal stability
Authors: Hintzen Dorine C. , Soto Mar , Schubert Michael , Bakker Bjorn , Spierings Diana C. J. , Szuhai Karoly , Lansdorp Peter M. , Kluin Roel J. C. , Foijer Floris , Medema René H. , Raaijmakers Jonne A.
Published: July 2022

Aneuploidy and chromosomal instability are both commonly found in cancer. Chromosomal instability leads to karyotype heterogeneity in tumors and is associated with therapy resistance, metastasis and poor prognosis. It has been hypothesized that aneuploidyper seis sufficient to drive CIN, however due to limited models and heterogenous results, it has remained controversial which aspects of aneuploidy can drive CIN. In this study we systematically tested the impact of different types of aneuploidies on the induction of CIN. We generated a plethora of isogenic aneuploid clones harboring whole chromosome or segmental aneuploidies in human p53-deficient RPE-1 cells. We observed increased segregation errors in cells harboring trisomies that strongly correlated to the number of gained genes. Strikingly, we found that clones harboring only monosomies do not induce a CIN phenotype. Finally, we found that an initial chromosome breakage event and subsequent fusion can instigate breakage-fusion-bridge cycles. By investigating the impact of monosomies, trisomies and segmental aneuploidies on chromosomal instability we further deciphered the complex relationship between aneuploidy and CIN.
Clinical and survival differences during separate COVID-19 surges: Investigating the impact of the Sars-CoV-2 alpha variant in critical care patients
Authors: Ritchie Andrew I. , Kadwani Owais , Saleh Dina , Baharlo Behrad , Broomhead Lesley R. , Randell Paul , Waheed Umeer , Templeton Maie , Brown Elizabeth , Stümpfle Richard , Patel Parind , Brett Stephen J. , Soni Sanooj
Published: July 2022

A number of studies have highlighted physiological data from the first surge in critically unwell Covid-19 patients but there is a paucity of data describing emerging variants of SARS-CoV-2, such as B.1.1.7. We compared ventilatory parameters, biochemical and physiological data and mortality between the first and second COVID-19 surges in the United Kingdom, where distinct variants of SARS-CoV-2 were the dominant stain. We performed a retrospective cohort study investigating critically unwell patients admitted with COVID-19 across three tertiary regional ICUs in London, UK. Of 1782 adult ICU patients screened, 330 intubated and ventilated patients diagnosed with COVID-19 were included. In the second wave where B.1.1.7 variant was the dominant strain, patients were had increased severity of ARDS whilst compliance was greater (p<0.05) and d-dimer lower. The 28-day mortality was not statistically significant (1st wave: 42.2% vs 2nd wave: 39.8%). However, when adjusted for key covariates, the hazard ratio for 28-day mortality in those patients with B.1.1.7 was 3.79 (CI 1.04–13.8; p = 0.043) compared to the original strain. During the second surge in the UK, where the COVID-19 variant B.1.1.7 was most prevalent, significantly more patients presented to critical care with severe ARDS. Furthermore, mortality risk was significantly greater in our ICU population during the second wave of the pandemic in those patients with B.1.1.7. As ICUs are experiencing further waves (particularly by the delta (B.1.617.2) variant), we highlight the urgent need for prospective studies describing immunological and pathophysiological differences across novel emerging variants.
Seasonal roost selection and activity of a remnant population of northern myotis in Pennsylvania
Authors: Lewis Mattea A. , Turner Gregory G. , Scafini Michael R. , Johnson Joseph S.
Published: July 2022

The decline in northern myotis (Myotis septentrionalis) populations due to the disease white-nose syndrome (WNS) has led to the species receiving federal protection in the United States and Canada, requiring conservation of critical habitats. However, considerably more is known about summer habitat preferences of northern myotis compared to late summer through winter. Our goal was to describe the seasonal presence and habitat use of a remnant colony of northern myotis in central Pennsylvania. We radio-tagged 31 northern myotis and established 6 acoustic monitoring stations to document activity from 2017–2021. We found that roost trees used during the maternity season by reproductive females were occupied by bats during both summer (21 June–14 August) and autumn (15 August–31 October), indicating similar habitat use patterns between seasons. During this time, both males and females preferred to roost in dead and declining trees. No other variable influenced male use, but females also preferred trees located close to water and in forest stands with higher basal area than randomly located trees. Northern myotis with active transmitters never left the study area and were tracked to roosts until early November. During October and November, a female and male were tracked to an underground network of air-filled voids (the Milieu Souterrain Superficiel) we presume to be a hibernaculum. Northern myotis calls were recorded outside this roost between March and October, and bats were observed emerging from this roost during spring and autumn but not summer. Acoustic activity at this site exhibited a seasonal pattern that differed from acoustic activity near roost trees and foraging areas, with a peak of activity during late summer when northern myotis are known to swarm. These data show that northern myotis maternity roosts are used extensively outside of summer and may be vulnerable to forestry practices that occur even outside of the pup-rearing season. These data also support the growing evidence that some northern myotis hibernate outside of caves and mines.
Award rate inequities in biomedical research
Authors: Zimmermann Alessandra , Klavans Richard , Offhaus Heather M. , Grieb Teri A. , Smith Caleb
Published: July 2022

Purpose

The analysis of existing institutional research proposal databases can provide novel insights into science funding parity. The purpose of this study was to analyze the relationship between race/ethnicity and extramural research proposal and award rates across a medical school faculty and to determine whether there was evidence that researchers changed their submission strategies because of differential inequities across submission categories.

Method

The authors performed an analysis of 14,263 biomedical research proposals with proposed start dates between 2010–2022 from the University of Michigan Medical School, measuring the proposal submission and award rates for each racial/ethnic group across 4 possible submission categories (R01 & Equivalent programs, other federal, industry, and non-profit).

Results

Researchers from each self-identified racial/ethnic group (Asian, Black/African American, Hispanic/Latino) pursued a different proposal submission strategy than the majority group (White). The authors found that Black/African American researchers experienced negative award rate differentials across all submission categories, which resulted in the lowest R01 & Equivalent and Other Federal submission rates of any racial/ethnic group and the highest submission rate to non-profit sources. The authors did not find support for the hypothesis that researchers changed submission strategies in response to award rate inequalities across submission categories.

Conclusions

Biomedical researchers from different racial/ethnic groups follow markedly different proposal submission strategies within the University of Michigan Medical School. There is also a clear relationship between race/ethnicity and rates of proposal award. Black/African American and Asian researchers appear disadvantaged across all submission categories relative to White researchers. This study can be easily replicated by other academic research institutions, revealing opportunities for positive intervention.
Agreement between maximum and mean handgrip strength measurements in cancer patients
Authors: de Almeida Marques Rayne , de Souza Vanusa Felício , do Rosario Thainá Cezini , da Silva Garcia Maria Rita Pereira , Pereira Taísa Sabrina Silva , Marques-Rocha José Luiz , Guandalini Valdete Regina
Published: July 2022

Background and aim

Handgrip strength (HGS) can be used to identify probable sarcopenia, by measuring maximum strength and/or through the average of three measurements. This study analyzed the agreement between maximum and mean HGS measurements in identifying probable sarcopenia in cancer patients.

Methods

Adult individuals of both sexes diagnosed with malignant neoplasm were evaluated. HGS (kg/f) was measured in both hands and nutritional status defined by the Patient-Generated Subjective Global Assessment (PG-SGA). Bland-Altman, Intraclass Correlation Coefficient (ICC), and Cronbach’s Alpha tests were applied to assess the agreement between measurements.

Results

One hundred forty-one patients aged 60.0 ± 14.2 years were evaluated. There was a predominance of elderly (57.4%), male (53.2%), and non-white (58.2%) individuals, with tumors located in the lower gastrointestinal tract (GIT) (36.9%) and with suspected or some degree of malnutrition (61.0%). For men, the Bland-Altman test showed a mean error of 1.37 (95% CI—1.03 to 3.80) for dominant HGS (DHGS) and 1.50 (95% CI—1.60 to 4.60) for non-dominant HGS (NDHGS), while for women the values were 1.34 (95% CI—0.27 to 2.95) and 1.14 (95% CI—1.10 to 3.39), respectively. The ICC showed excellent reproducibility (> 0.90) and the Cronbach’s Alpha was satisfactory (0.99).

Conclusion

Despite the satisfactory agreement observed between maximum and mean HGS values, in this study, individuals of both sexes with probable sarcopenia were better identified through mean values.
Explosive and implosive root concepts: An analysis of music moods rooted by two influential rap artists
Authors: Nagayama Susumu , Mitsuhashi Hitoshi
Published: July 2022

This study proposes the notion of “root concepts” in cultural production, defined as a novel style and mode that a creator expresses at the initial field development phase, and that has a great influence on subsequent creators. We explore the role of root concepts in cultural evolution by focusing on their capacity to generate new combinations with other elements and examine how creators use root concepts jointly with other elements. Using data on artists and albums in the rap genre from the online database Allmusic, we view music moods as a type of experience that music generates and focus on music moods as a phenotype in studying styles and modes. We constructed a dataset of recombinatory patterns in the subsequent cultural production and identified two types of root concepts: implosive concepts, which artists use jointly with similar elements; and explosive concepts, which artists use in conjunction with highly diversified elements. Implosive concepts are exclusive because they require creators to have network contagions with those familiar with the root concepts and have strong and specific socio-economic identities. Previous research has suggested that finding a new combination is challenging owing to creators’ limited cognitive capacities and the resulting local search. Our finding presents an alternative explanation: some root concepts (i.e., implosive ones) possess innate characteristics that limit creators from experimentally integrating diversified elements. This study develops new opportunities for future research on the evolutionary growth of cultural production and knowledge fields.
Externally validated models for first diagnosis and risk of progression of knee osteoarthritis
Authors: McCabe Philippa Grace , Lisboa Paulo , Baltzopoulos Bill , Olier Ivan
Published: July 2022

Objective

We develop and externally validate two models for use with radiological knee osteoarthritis. They consist of a diagnostic model for KOA and a prognostic model of time to onset of KOA. Model development and optimisation used data from the Osteoarthritis initiative (OAI) and external validation for both models was by application to data from the Multicenter Osteoarthritis Study (MOST).

Materials and methods

The diagnostic model at first presentation comprises subjects in the OAI with and without KOA (n = 2006), modelling with multivariate logistic regression. The prognostic sample involves 5-year follow-up of subjects presenting without clinical KOA (n = 1155), with modelling with Cox regression. In both instances the models used training data sets of n = 1353 and 1002 subjects and optimisation used test data sets of n = 1354 and 1003. The external validation data sets for the diagnostic and prognostic models comprised n = 2006 and n = 1155 subjects respectively.

Results

The classification performance of the diagnostic model on the test data has an AUC of 0.748 (0.721–0.774) and 0.670 (0.631–0.708) in external validation. The survival model has concordance scores for the OAI test set of 0.74 (0.7325–0.7439) and in external validation 0.72 (0.7190–0.7373). The survival approach stratified the population into two risk cohorts. The separation between the cohorts remains when the model is applied to the validation data.

Discussion

The models produced are interpretable with app interfaces that implement nomograms. The apps may be used for stratification and for patient education over the impact of modifiable risk factors. The externally validated results, by application to data from a substantial prospective observational study, show the robustness of models for likelihood of presenting with KOA at an initial assessment based on risk factors identified by the OAI protocol and stratification of risk for developing KOA in the next five years.

Conclusion

Modelling clinical KOA from OAI data validates well for the MOST data set. Both risk models identified key factors for differentiation of the target population from commonly available variables. With this analysis there is potential to improve clinical management of patients.
Does knowing the influenza epidemic threshold has been reached influence the performance of influenza case definitions?
Authors: Soldevila Núria , Toledo Diana , Martínez Ana , Godoy Pere , Torner Núria , Rius Cristina , Jané Mireia , Domínguez Angela ,   the PIDIRAC Sentinel Surveillance Program of Catalonia
Published: July 2022

Background

Disease surveillance using adequate case definitions is very important. The objective of the study was to compare the performance of influenza case definitions and influenza symptoms in the first two epidemic weeks with respect to other epidemic weeks.

Methods

We analysed cases of acute respiratory infection detected by the network of sentinel primary care physicians of Catalonia for 10 seasons. We calculated the diagnostic odds ratio (DOR) and 95% confidence intervals (CI) for the first two epidemic weeks and for other epidemic weeks.

Results

A total of 4,338 samples were collected in the epidemic weeks, of which 2,446 (56.4%) were positive for influenza. The most predictive case definition for laboratory-confirmed influenza was the WHO case definition for influenza-like illness (ILI) in the first two epidemic weeks (DOR 2.10; 95% CI 1.57–2.81) and in other epidemic weeks (DOR 2.31; 95% CI 1.96–2.72). The most predictive symptom was fever. After knowing that epidemic threshold had been reached, the DOR of the ILI WHO case definition in children aged <5 years and cough and fever in this group increased (190%, 170% and 213%, respectively).

Conclusions

During influenza epidemics, differences in the performance of the case definition and the discriminative ability of symptoms were found according to whether it was known that the epidemic threshold had been reached or not. This suggests that sentinel physicians are stricter in selecting samples to send to the laboratory from patients who present symptoms more specific to influenza after rather than before an influenza epidemic has been declared.
Sparse representation of brain signals offers effective computation of cortico-muscular coupling value to predict the task-related and non-task sEMG channels: A joint hdEEG-sEMG study
Authors: Keihani Ahmadreza , Mohammadi Amin Mohammad , Marzbani Hengameh , Nafissi Shahriar , Haidari Mohsen Reza , Jafari Amir Homayoun
Published: July 2022

Cortico-muscular interactions play important role in sensorimotor control during motor task and are commonly studied by cortico-muscular coherence (CMC) method using joint electroencephalogram-surface electromyogram (EEG-sEMG) signals. As noise and time delay between the two signals weaken the CMC value, coupling difference between non-task sEMG channels is often undetectable. We used sparse representation of EEG channels to compute CMC and detect coupling for task-related and non-task sEMG signals. High-density joint EEG-sEMG (53 EEG channels, 4 sEMG bipolar channels) signals were acquired from 15 subjects (30.26 ± 4.96 years) during four specific hand and foot contraction tasks (2 dynamic and 2 static contraction). Sparse representations method was applied to detect projection of EEG signals on each sEMG channel. Bayesian optimization was employed to select best-fitted method with tuned hyperparameters on the input feeding data while using 80% data as the train set and 20% as test set. K-fold (K = 5) cross-validation method was used for evaluation of trained model. Two models were trained separately, one for CMC data and the other from sparse representation of EEG channels on each sEMG channel. Sensitivity, specificity, and accuracy criteria were obtained for test dataset to evaluate the performance of task-related and non-task sEMG channels detection. Coupling values were significantly different between grand average of task-related compared to the non-task sEMG channels (Z = -6.33, p< 0.001, task-related median = 2.011, non-task median = 0.112). Strong coupling index was found even in single trial analysis. Sparse representation approach (best fitted model: SVM, Accuracy = 88.12%, Sensitivity = 83.85%, Specificity = 92.45%) outperformed CMC method (best fitted model: KNN, Accuracy = 50.83%, Sensitivity = 52.17%, Specificity = 49.47%). Sparse representation approach offers high performance to detect CMC for discerning the EMG channels involved in the contraction tasks and non-tasks.
Aspalathus linearis suppresses cell survival and proliferation of enzalutamide-resistant prostate cancer cells via inhibition of c-Myc and stability of androgen receptor
Authors: Wang Bi-Juan , Huang Shih-Han , Kao Cheng-Li , Muller Christo J. F. , Wang Ya-Pei , Chang Kai-Hsiung , Wen Hui-Chin , Yeh Chien-Chih , Shih Li-Jane , Kao Yung-Hsi , Huang Shu-Pin , Li Chia-Yang , Chuu Chih-Pin
Published: July 2022

Enzalutamide, a nonsteroidal antiandrogen, significantly prolonged the survival of patients with metastatic castration-resistant prostate cancer (CRPC). However, patients receiving enzalutamide frequently develop drug resistance. Rooibos (Aspalathus linearis) is a shrub-like leguminous fynbos plant endemic to the Cedarberg Mountains area in South Africa. We evaluated the possibility of using a pharmaceutical-grade green rooibos extract (GRT, containing 12.78% aspalathin) to suppress the proliferation and survival of enzalutamide-resistant prostate cancer (PCa) cells. Treatment with GRT dose-dependently suppressed the proliferation, survival, and colony formation of enzalutamide-resistant C4-2 MDV3100r cells and PC-3 cells. Non-cancerous human cells were more resistant to GRT treatment. GRT suppressed the expression of proteins involved in phosphoinositide 3-kinase (PI3K)-Akt signaling, androgen receptor (AR), phospho-AR (Ser81), cyclin-dependent kinase 1 (Cdk1), c-Myc and Bcl-2 but increased the expression of apoptotic proteins. Overexpression of c-Myc antagonized the suppressive effects of GRT, while knockdown of c-Myc increased the sensitivity of PCa cells to GRT treatment. Expression level of c-Myc correlated to resistance of PCa cells to GRT treatment. Additionally, immunofluorescence microscopy demonstrated that GRT reduced the abundance of AR proteins both in nucleus and cytoplasm. Treatment with cycloheximide revealed that GRT reduced the stability of AR. GRT suppressed protein expression of AR and AR’s downstream target prostate specific antigen (PSA) in C4-2 MDV3100r cells. Interestingly, we observed that AR proteins accumulate in nucleus and PSA expression is activated in the AR-positive enzalutamide-resistant PCa cells even in the absence of androgen. Our results suggested that GRT treatment suppressed the cell proliferation and survival of enzalutamide-resistant PCa cells via inhibition of c-Myc, induction of apoptosis, as well as the suppression of expression, signaling and stability of AR. GRT is a potential adjuvant therapeutic agent for enzalutamide-resistant PCa.
Economic impact of a machine learning-based strategy for preparation of blood products in brain tumor surgery
Authors: Tunthanathip Thara , Sae-heng Sakchai , Oearsakul Thakul , Kaewborisutsakul Anukoon , Taweesomboonyat Chin
Published: July 2022

Background

Globally, blood donation has been disturbed due to the pandemic. Consequently, the optimization of preoperative blood preparation should be a point of concern. Machine learning (ML) is one of the modern approaches that have been applied by physicians to help decision-making. The main objective of this study was to identify the cost differences of the ML-based strategy compared with other strategies in preoperative blood products preparation. A secondary objective was to compare the effectiveness indexes of blood products preparation among strategies.

Methods

The study utilized a retrospective cohort design conducted on brain tumor patients who had undergone surgery between January 2014 and December 2021. Overall data were divided into two cohorts. The first cohort was used for the development and deployment of the ML-based web application, while validation, comparison of the effectiveness indexes, and economic evaluation were performed using the second cohort. Therefore, the effectiveness indexes of blood preparation and cost difference were compared among the ML-based strategy, clinical trial-based strategy, and routine-based strategy.

Results

Over a 2-year period, the crossmatch to transfusion (C/T) ratio, transfusion probability (Tp), and transfusion index (Ti) of the ML-based strategy were 1.10, 57.0%, and 1.62, respectively, while the routine-based strategy had a C/T ratio of 4.67%, Tp of 27.9%%, and Ti of 0.79. The overall costs of blood products preparation among the ML-based strategy, clinical trial-based strategy, and routine-based strategy were 30, 061.56$, 57,313.92$, and 136,292.94$, respectively. From the cost difference between the ML-based strategy and routine-based strategy, we observed cost savings of 92,519.97$ (67.88%) for the 2-year period.

Conclusion

The ML-based strategy is one of the most effective strategies to balance the unnecessary workloads at blood banks and reduce the cost of unnecessary blood products preparation from low C/T ratio as well as high Tp and Ti. Further studies should be performed to confirm the generalizability and applicability of the ML-based strategy.
Staphylococcus aureus-induced immunosuppression mediated by IL-10 and IL-27 facilitates nasal colonisation
Authors: Kelly Alanna M. , Leech John M. , Doyle Sarah L. , McLoughlin Rachel M.
Published: July 2022

Staphylococcus aureus persistently colonises the anterior nares of a significant proportion of the healthy population, however the local immune response elicited during S. aureus nasal colonisation remains ill-defined. Local activation of IL-17/IL-22 producing T cells are critical for controlling bacterial clearance from the nasal cavity. However, recurrent and long-term colonisation is commonplace indicating efficient clearance does not invariably occur. Here we identify a central role for the regulatory cytokine IL-10 in facilitating bacterial persistence during S. aureus nasal colonisation in a murine model. IL-10 is produced rapidly within the nasal cavity following S. aureus colonisation, primarily by myeloid cells. Colonised IL-10-/- mice demonstrate enhanced IL-17+ and IL-22+ T cell responses and more rapidly clear bacteria from the nasal tissues as compared with wild-type mice. S. aureus also induces the regulatory cytokine IL-27 within the nasal tissue, which acts upstream of IL-10 promoting its production. IL-27 blockade reduces IL-10 production within the nasal cavity and improves bacterial clearance. TLR2 signalling was confirmed to be central to controlling the IL-10 response. Our findings conclude that during nasal colonisation S. aureus creates an immunosuppressive microenvironment through the local induction of IL-27 and IL-10, to dampen protective T cell responses and facilitate its persistence.
Las mujeres muertas de Odiseo: almas de silente locuacidad
Authors: Gómez Pilar
Published: July 2022

En la compleja estructura de la Odisea, siempre una mujer marca jalones decisivos en el retorno de Odiseo a Ítaca, y deviene esencial para configurar un relato cuyo protagonista de forma paulatina debe reingresar en el hilo de la narración y recobrar su identidad. Por ello, los espacios determinantes en esta restitución están vinculados a nombres femeninos.
Este trabajo se centra en el espacio de Odisea más antitético al mundo real, el inframundo, donde no solo las palabras de Tiresias preparan al héroe para culminar con éxito su regreso, sinó también la voz y el silencio de las mujeres muertas. La katábasis del canto once es uno de los episodios del poema que mejor señala la condición de Odiseo como héroe de límites. Ante el plural universo femenino de la Odisea, las sombras de las heroínas que Odiseo contempla en el Hades tal vez no son solo una interpolación o un guiño a un potencial público femenino. Si se observa quiénes son las féminas mencionadas, qué recuerda de ellas el poeta y cómo son caracterizadas, quizá la selección de heroínas –en particular aquellas que son perversas y aquellas otras que solo son mencionadas– no es casual y pueda conjeturarse la inclusión de todas ellas como un refuerzo para singularizar, por contraste, a la propia Penélope
Duas Passagens Camonianas
Authors: B. Penafiel André
Published: July 2022

O artigo discute o sentido literal de duas estâncias d’Os Lusíadas, de Camões, questionando se as leituras tradicionalmente oferecidas pelos editores estariam corretas ou se devemos admitir pequenas emendas textuais. A primeira passagem (Canto II, estância 75) respeita ao infinitivo flexionado; a segunda passagem (Canto IX, estância 70) envolve a pontuação. A análise de cada passagem inclui discussões sobre gramática e semântica. Dicionários e outras passagens de Camões foram consultados. Os resultados alcançados foram distintos. Quanto à passagem do Canto II, parece preferível manter a leitura tradicional. Embora não se alcance uma leitura original do texto camoniano, o percurso percorrido merece ser registrado, na medida em que esclarece um pouco a gramática da língua portuguesa, além de documentar o uso de Camões. Já a passagem do Canto IX merece ser pontuada de maneira ligeiramente diferente. Alguns editores parecem ter se equivocado quanto ao sentido pretendido pelo Poeta e a análise soluciona este pequeno problema textual de maneira satisfatória. A discussão de duas passagens e o cotejo de diversas edições, que pouco diferem entre si, convida, na conclusão, a uma pergunta mais ampla, a saber, se há necessidade de reeditar Os Lusíadas ou se as edições existentes satisfazem. O estudo, apesar de modesto, deverá atrair o interesse de estudiosos, bem como de amantes da língua portuguesa e da poesia de Camões que poderão, a partir dele, refletir sobre aspectos que não lhe ocorreriam espontaneamente. Convida também à saudável reflexão sobre o papel e a necessidade de novas edições d’Os Lusíadas.