bluuebunny/crossref_metadata_2025 huggingface dataset:
105 Feed Additives That Optimize Nitrogen Bio-availability in Nursery Pigs Fed Reducing Crude Protein Diets
Authors: Tsai Tsung Cheng Cheng
Published: May 2021

Reducing dietary crude protein in early nursery has been suggested as a way to conquer the challenge of post-weaning diarrhea. In order to meet the amino acid requirement in RCP diets, feed grade amino acids are typically used, but the detrimental effect on growth performance associated with the aggressive usage of these amino acids resulted in the establishment of dietary limits. Given the fact that protein deposition rate is the highest in young pigs, lower amino acid consumption could slow the growth rate and feed efficiency in early nursery phase. Therefore, identifying feed additives that increase nutrient retention not only can further reduce dietary nitrogen but also maintain GI tract health. Among all feed additives that exert improvements on nutrient digestibility, peptide and organic acids will be discussed here. Peptide, a di- tri amino acid, is absorbed more efficiently than free amino acids in the small intestine through a peptide transporter at the enteric site. In addition, aside from meeting the amino acid requirement, the bioactive peptide possesses functions such as antimicrobial, antioxidant, anti-inflammatory, and as a stimulated Peptide transporter associated with nutrient absorption. Hence, a dietary peptide can help pigs meet the nutrient requirement in RCP diets. Organic acids, on the other hand, have been found to lower gastric pH which improves nutrient absorption, alters microbiome structure (via bacteriostatic and bactericidal) and promotes the growth and health of animals. In addition, organic acids are a ready energy source which is especially critical since nutrient supply disruption, due to low intake during early weaning, is commonly found in weaning pigs. It is noteworthy that variations of growth-promoting effects do exist for both peptide and organic acid products. Thus, understanding the mode of action and response of dietary peptide and organic acid supplements when used across different farm settings is important to help producers decide when to transfer to an antibiotic-free, low zinc operation.
189 Fundamentals, Common Mistakes, and Graduate Education in Statistics
Authors: Serão Nick V , Tokach Mike D , Paton Neil
Published: May 2021

Experimental design and statistical data analyses are fundamental components of animal science research. Proper design of experiments and adequate sampling permits testing hypotheses raised by researchers and sets the stage for collecting required data and subsequent statistical analysis. When designing experiments, researchers should respect rules of randomization of treatments to avoid statistical bias and permit proper inference to be drawn. Use of sample sizes that result in adequate statistical power to identify the hypothesized differences among factor levels of interest is key and should be driven by formal processes determining such. Best practices for data collection should be performed to obtain high quality data by reducing collection (e.g., mislabeling, improper technique) and measurement errors. With sound data, appropriate and optimal statistical methods should be used to generate valid results. The statistical method deployed should be chosen based on assumptions about residuals (e.g., normality, correlation, and homogeneity) and on the type of data (e.g., quantitative continuous or categorical). The appropriate statistical model used should also be consistent with the experimental design to validate the respective test statistics. The science of statistics is changing rapidly. With the development of high-throughput technologies, the generation of large datasets, high performance and sophisticated models and the interest in Big Data, the training of animal science graduate students in data management and rigorous statistical analyses is more important than ever. In order to meet the demands of current trends, animal science graduate students must be trained in several complex statistical and computational skills to meet the challenges imposed by these complicated, sophisticated and nuanced analytical methods. The livestock production sector will benefit from improved training, use of advanced and appropriate experimental designs, and collection and analysis of quality data in research.
260 Growth Performance and Gut Integrity of Nursery Pigs Fed Diet with butyric Acid and Enzymatically Hydrolyzed Yeast Product
Authors: Hong Jinsu , Kim Hee Seong , Patterson Rob , Woyengo Tofuko A
Published: May 2021

The objective of this study was to evaluate the effects of butyric acid (BA) and enzymatically hydrolyzed yeast product (EYP) on growth performance and gut health of nursery pigs. A total of 96 weaned pigs (initial body weight = 6.60 kg) were housed in 24 pens (4 pigs/pen) and fed 3 diets in a randomized complete block design. The diets were corn-soybean meal-based without or with 0.05% BA or 0.1% EYP. The diets were fed in 2 phases; Phase 1: d 0 to 7 and Phase 2: d 7 to 20. Growth performance and fecal score were determined by phase. One pig from in each pen was selected for measuring organ weights, gut permeability, and electrophysiological parameters of jejunum mounted in Ussing chambers. The selected pigs were sacrificed at a rate of 6 pigs (balanced for diets) per day from d 10 to 17. Dietary BA increased (P < 0.05) gain to feed ratio for d 7 to 20 by 5.8% and for entire study period by 15.8%. Fecal scores were unaffected by dietary BA or EYP. Dietary EYP increased (P < 0.05) weight of cecum as a proportion of live body weight by 36.4%. Dietary BA decreased (P < 0.05) the flow of fluorescein isothiocyanate-dextran from mucosal to serosal side of jejunum by 31.7%, implying that dietary BA reduced jejunal permeability. Jejunal short circuit current was decreased (P < 0.05) by dietary BA or EYP. However, jejunal trans-epithelial electrical resistance was unaffected by dietary BA or EYP. In conclusion, dietary BA improved feed efficiency and reduced jejunal permeability to fluorescein isothiocyanate-dextran, implying that it can improve the gut health of weaned pigs through reduced jejunal permeability to toxins. Dietary EYP increased caecum weight, implying that it can improve hindgut fermentation in weaned pigs.
254 Evaluation of Different Corn Milling Methods for High-moisture and Dry Corn on Finishing Cattle Performance and Carcass Characteristics
Authors: Coulson Caitlin A , Troyer Braden , McPhillips Levi J , Norman Mitch , Erickson Galen E
Published: May 2021

Steers (n=600; Initial BW = 402 ± 17 kg) were fed for134 day to evaluate the effect of milling method and corn type on performance and carcass characteristics. Treatments were evaluated as a 2 × 3 factorial with factors being milling method (Automatic Ag® roller mill or hammer mill) and corn type (100% high-moisture, 100% dry, or 50:50 blend of high-moisture and dry corn). High-moisture corn was processed at harvested based on respective treatment and ensiled until trial initiation. Both dry corn and HMC were processed using a 16-mm screen in the hammer mill and the roller mill was adjusted to ensure all kernels were broken. There were no interactions between milling method and corn type for final BW, daily gain (ADG), or dry matter intake (DMI; P ≥ 0.32), but there was a tendency for an interaction for G:F (P = 0.09). Cattle fed 100% high-moisture corn processed with the Automatic Ag roller mill were 4.7% more efficient (P ≤ 0.01) with 55% lower fecal starch (P < 0.01) compared to high-moisture corn processed with the hammer mill. Cattle fed dry corn tended (P = 0.07) to have a greater live final BW regardless of milling type and had the greatest DMI (P ≤ 0.01) Intake decreased as high-moisture corn was increased in the diet. Due to no differences in ADG with lower DMI led to a 6% improvement (P ≤ 0.01) in G:F for steers fed HMC. There were no further effects (P ≥ 0.14) on performance or carcass traits regardless of milling method or corn type. Processing high-moisture corn using Automatic Ag roller mill improved feed efficiency compared to processing with a hammer mill when corn was included at 70% of the diet, but processing method had little effect when fed as dry corn or blended diets.
276 Evaluation of a Long-acting Growth-promoting Implant (Revalor-XS) as an Initial Implant in a Re-implant Program with a revalor-200 Terminal Implant in Feedlot Cattle: A Three-study Pooled Analysis
Authors: Crawford Grant I , Nichols Wade T , Hutcheson John P , Streeter Marshall N , Nuttelman Brandon L , Walter Lee-Anne J , Renter David G , Amrine David
Published: May 2021

Data from three large-pen feedlot studies were pooled to evaluate use of a long-acting implant (Revalor-XS) as an initial implant in a re-implant program. The three studies consisted of 2,764 steers in 40 pens, with an initial body weight (BW) of 271 kg. Treatments consisted of Revalor-IS [80 mg trenbolone acetate (TBA), 16 mg estradiol (E2)] administered on arrival, followed by Revalor-200 (200 mg TBA, 20 mg E2) terminal implant (IS/200) or Revalor-XS (80 mg TBA, 16 mg E2 uncoated; 120 mg TBA, 24 mg E2 coated; 200 mg TBA, 40 mg E2 total) on arrival followed by Revalor-200 terminal implant (XS/200). Steers were fed to equal days-on-feed (DOF) within study and averaged 210 DOF. Steers within treatment were re-implanted at same DOF in two studies (Day 124 on average followed by an 81-day terminal implant window). In the third study, IS/200 steers were implanted on Day 120 and XS/200 steers on Day 140 of the 217-day study. Final BW averaged 635.6 and 638.3 kg for IS/200 and XS/200, respectively (P = 0.21). There were no differences (P > 0.26) in DMI, ADG and Gain:Feed between treatments. Hot carcass weight tended (P = 0.07) to be greater with XS/200 (412.7 kg) compared with IS/200 (409.1 kg). Ribeye area was greater (P < 0.01) and fat thickness tended (P = 0.06) to be lower with XS/200 compared with IS/200. Distributions of USDA quality grades were not affected (P = 0.26) by treatment. Distributions of USDA yield grades (YG) were affected (P = 0.01) by treatment with a shift toward more YG 1 and 2 carcasses with XS/200 and more YG 4 and 5 carcasses with IS/200. This analysis indicates that using Revalor-XS as an initial implant leads to greater carcass weight and ribeye area and lower YG when compared with Revalor-IS.
272 Managing for Efficiency and Quality Through Diet and Implant Strategies on Steers Selected for Superior Marbling
Authors: Lundy Erika L , Beenken Aubree M , Wall Patrick B , Loy Daniel D
Published: May 2021

A 144-day study assessed the effects of dietary energy and implant potency to determine optimum strategies for managing feed conversion (F:G) and marbling. Fifty-four Angus steers (327 ± 8 kg) from Iowa State University’s herd genetically selected for enhanced marbling were stratified by initial bodyweight (BW), ultrasound intramuscular fat, and age to a 2 × 3 factorial. Dietary treatments included: low energy (1.30 Mcal NEg/kg DM, 18% roughage level; LE) or high energy finishing ration (1.39 Mcal NEg/kg DM, 8% roughage; HE). Implant treatments (IMP; Merck) included: no implant (NOIMP), Revalor-IS (RIS), or Revalor-200 (R200) on d 0 and 74. Steers were fed via bunks capturing daily individual feed disappearance (n = 9 steers/treatment). Steers were weighed on d 0, 74, and 144 and harvested on d 145. Data were analyzed in Proc Mixed of SAS with fixed effects of diet, IMP, and interaction. No interactions were observed for feedlot performance (P > 0.17). Steers fed HE had greater average daily gain (ADG) and final BW than LE steers (P < 0.01) while LE steers had greater F:G (P = 0.04). Final BW and ADG were greatest for R200, intermediate for RIS, and lowest for NOIMP (P < 0.01). F:G was greatest for NOIMP, intermediate for RIS, and lowest for R200 (P < 0.01). Steers fed HE had increased ribeye area (P < 0.01) and tended to have greater marbling score (P = 0.06; 809) than LE steers (769). While ribeye area increased in response to implant potency (P < 0.01), marbling score was not impacted (P = 0.21) by IMP (815, 771, 782, for NOIMP, RIS, R200, respectively). Overall, steers graded 100% Choice or higher and 55% Prime. These data suggest implants, when used appropriately, improve growth performance and efficiency in beef steers without compromising carcass quality.
145 Effect of Substandard Teats on Piglet and Sow Performance
Authors: Knauer Mark , Peppmeier Zack
Published: May 2021

The objective of the study was to evaluate sow teat quality in relation to subsequent reproductive throughput. Data included one cohort of 42 Landrace × Large White second parity maternal line sows at the Tidewater Research Station (Plymouth, NC). Functional teats (FUNCTIONAL) were classified pre-farrow as acceptable (ACCEPTABLE) or substandard (SUBSTANDARD). Teats were categorized as SUBSTANDARD when teat size was ≤75% of ACCEPTABLE. At day 20 of lactation, ACCEPTABLE and SUBSTANDARD were assessed for the presence of a swollen mammary gland and piglets observed nursing SUBSTANDARD were recorded (24 piglets from 15 litters). Means for FUNCTIONAL, ACCEPTABLE and SUBSTANDARD were 15.07, 13.59 and 1.48 teats, respectively. Biological dam traits included birth weight (BWT), total number born (TNB), litter size at weaning (LSW) and piglet survival (LSW/TNB). Weaning weight (WWT) was considered a trait of the nurse dam. Means for TNB, LSW, litter BWT and litter WWT were 13.4, 10.9, 17.8 kg and 57.8 kg, respectively. Data was analyzed using a chi-square test for binary traits and linear mixed models for continuous traits. At weaning, a greater (P < 0.01) proportion of ACCEPTABLE had a functional mammary gland when compared to SUBSTANDARD (76 vs. 47%). Within litters, piglets nursing SUBSTANDARD tended (em>P = 0.09) to be 158 grams lighter at weaning in comparison to ACCEPTABLE. Yet BWT of SUBSTANDARD piglets did not differ (em>P = 0.60) from piglets nursing ACCEPTABLE. Across litters, an increase in one SUBSTANDARD tended (em>P = 0.07) to reduce litter WWT by 1.74 kilograms. An increase in one SUBSTANDARD tended (em>P < 0.07) to increase piglet survival by 3.5%. Results suggest substandard teats, identified pre-farrow, are less likely to have a functional mammary gland at weaning, impair piglet quality yet may enhance piglet survival.
236 Performance and Antibiotic Use of Piglets Vaccinated with an E. coli F4/F18 Vaccination for the Prevention of F18-ETEC Post-weaning Diarrhea
Authors: Vangroenweghe Frédéric A , Van Poucke Annelies , Defoort Pascal
Published: May 2021

Post-weaning Escherichia coli diarrhea (PWD) remains a major cause of economic losses for the pig industry. PWD, caused by enterotoxigenic E. coli (ETEC), typically provokes mild to severe watery diarrhea between 5 and 10 days after weaning. Recently, an oral live bivalent E. coli F4/F18 vaccine (Coliprotec® F4/F18; Elanco) is available on the European market, which reduces the impact of PWD provoked by F4-ETEC and F18-ETEC. The objective was to compare technical results of E. coli F4/F18 vaccination with previous standard therapeutic approach under field conditions. A 1100-sow farm with diagnosed problems of PWD due to F18-ETEC was selected. Control piglets received the standard treatment protocol with antimicrobials during the post-weaning phase. Vaccinated piglets were immunized at 18 days with the oral live bivalent E. coli F4/ F18 vaccine. At weaning, no standard group medication (ZnO and antibiotics) was applied for prevention of PWD. Piglets were fed a commercial dry feed. Several performance parameters were collected: weight at d0-47, ADWG, ADFI, FCR, TI100 and mortality. Statistical analysis was performed with JMP 14.0 – comparison of means. Oral E. coli F4/F18 vaccination significantly reduced the mortality rate (3.56% to 1.67%; P< 0.05) and TI100 (10 to 0 days; P< 0.05). All other performance parameters (ADWG, ADFI and FCR) were at the same level compared to pre-vaccination. Live E. coli F4/F18 vaccination against PWD resulted in similar technical performance parameters, in combination with a significant reduction in the mortality and medication use. In conclusion, control of PWD through vaccination is a good option in order to prevent piglets from the negative clinical outcomes of F18-ETEC infection during the post-weaning period.
16 What’s a Connected Barn and How Do We Use It?
Authors: Ramirez Brett
Published: May 2021

Internet enabled swine facilities with smart controllers are forging the future directions of swine management, data analytics, and research. Today’s smart controllers have capabilities far beyond just ventilation operation, they are sophisticated data acquisition and control systems with cloud-based platforms for data aggregation and visualization. This technology has promoted the concept of a connected barn – a facility with autonomous, real-time data capture, analysis, and decision making. Connected barns can monitor indoor environmental conditions, personnel traffic, ventilation function, power consumption, bin weights, weather, refrigerators, etc. This new abundance of customizable measurement options sampled at a high-resolution will fundamentally change swine production. There is often a general lack of information related to conditions/events the pigs experience inside a barn that impact short-/long-term productivity and provide context for interpreting conventional data. Information is now superimposable with other data streams and new relationships between, for example, pig activity, environment, feed intake, daily gain, water consumption, etc. can be discovered. This will enable new avenues in nutrition, health, genetics, technology, husbandry, and engineering because a unified data stream, across numerous spaces is possible. Connected barns provide the link between pigs, data, and their environment. While opportunities are limitless, several key challenges need addressing: representativeness and accuracy of data; diligent management of advanced technology; and cultivation of transdisciplinary knowledge to understand limitations and make thoughtful inquiries. A connected barn is no longer a future concept, but a tangible production system ripe with a myriad of opportunities that will shape the course of animal production.
150 A Factorial Approach to Estimate Energy Requirements and Feeding Levels for Boars in Studs
Authors: Lu Ning , Kleve-Feld Michael , Wilson Mark E , Vier Carine M , Cast Wayne R , Navales Ron A , Dritz Steve S , Orlando Uislei A
Published: May 2021

Boars are a source of genetic improvement and influence farrowing rate and litter size. Overfeeding boars increases incidence of feet and leg problems and reduces libido, whereas underfeeding boars may decrease semen output and libido. To help boar stud personnel estimate the energy requirements and base feeding levels, an interactive web application named PIC Optimum Boar Feeding Tool has been developed. The application encompasses three components, including an input interface using dynamic models to estimate energy requirement, an output interface displaying recommended feeding program, and functional modules facilitating the ease of use. The input interface requests users to provide the start and final weight of boars in isolation, isolation length, boar stud room temperature, number of collections per week, and dietary energy level. The daily energy requirement (Mcal of ME/day) is calculated using the following energy-demanding factors: 1) maintenance, 0.1823 × (body weight, kg)0.665; 2) weight gain, 0.00489 × (average daily gain, kg/d); 3) each degree below 17°C for individually penned boars on slatted floors, 0.00382 × (body weight, kg)0.75; 4) mating activity, 0.0043 × (body weight, kg)0.75; and 5) sperm production, 0.1. These factors and the user-defined inputs including dietary energy, are used to calculate a daily feeding amount. The users also have the option to calculate the recommended feeding level for boars of specified body weight. Other functional modules include a flank-to-flank measurement tool that estimates boar body weight and customized downloadable nutrient specifications. This web application incorporates a spectrum of biological, nutritional, and management aspects to allow boar stud personnel to easily make boar feeding decisions.
49 Histomorphic Analysis of the Effect of Day and Level of Colostrum Intake on Jejunum Development
Authors: Sheets Jocelyn , Suarez-Trujillo Aridany , Teeple Kelsey , Senn L Kirsten , Stewart Kara R , Casey Theresa
Published: May 2021

The first milk sows synthesize is colostrum, and is only available to the neonate right after birth. Colostrum provides immunity, nutrients, energy, and bioactive factors which are essential for the survival, growth, and development of newborn piglets. The first few days after birth the gastrointestinal (GI) tract undergoes developmental changes and rapid growth in response to bioactive factors in milk. We hypothesized that amount of colostrum a neonate consumes the first 24 h postnatal effects the development of the GI tract. The objectives of this study were to measure the histomorphic growth of the jejunum between birth (day 0, D0) and postnatal day 7 (D7) and to determine the effect of ingesting a high versus low amount of colostrum. Gilts were identified at birth and immediately euthanized (D0, n = 6) or bottle fed a 24 h colostrum dose of 10% (COL10, n = 7) or 20% (COL20, n = 7) of birth body weight. Colostrum fed neonates were returned to birth sows after 24 h and allowed to nurse naturally until postnatal (D7), when gilts were euthanized. Gilts were dissected and jejunum was removed, weighed and placed in buffered formalin for preparation of histological sections. Five µm sections were mounted on glass slides and stained with hematoxylin and eosin (H&E). Images were captured using light microscopy at 10X magnification. ImageJ was used to measure villi length, width, stromal area, epithelial area, and crypt length. T-test analysis indicated that there was no difference between COL10 and COL20 in any of the morphological features (P > 0.05), however between D0 and D7 villi width, epithelial area, and crypt length increased (P < 0.05). Differences in histomorphology across the first week postnatal was not affected by level of colostrum intake in the first 24 h postnatal.
PSIII-1 Effect of Rearing Cross-fostered Piglets in Litters of Differing Size Relative to Sow Functional Teat Number on Pre-weaning Growth and Mortality
Authors: Vande Pol Katherine D , Rivas Rafael Ovidio Bautista , Espinal Alicia Olivo , Harper Heath , Shull Caleb M , Brown Katie , Ellis Michael
Published: May 2021

Litter sizes of commercial sows have increased recently, often resulting in the number of piglets exceeding the sow functional teat number. The objective of this study was to determine the effects of litter size after cross-fostering on piglet pre-weaning mortality (PWM) and growth. A RCBD was used with 13 blocks of 3 litters (total 39 litters/561 piglets); blocking factors were farrowing day, sow parity, body condition score, and functional teat number. Three Litter Size treatments (LS) relative to sow functional teat number were compared: Under (2 piglets below); Equal (same number of piglets); Over (2 piglets above). Piglets were weighed 24 h after birth and allotted to LS to create litters with similar gender ratio and average and CV of birth weight. Weaning was at 19.5 ± 0.50 d, weights and PWM were recorded. Piglet weight data were analyzed using PROC MIXED of SAS; PWM data were analyzed using PROC GLIMMIX of SAS. Models included LS and sow within block. Litter sizes averaged 12.1, 14.1, and 16.1 for the Under, Equal, and Over treatments, respectively (P ≤ 0.05). The Under treatment tended (P = 0.07) to have greater weaning weights compared to the Equal and Over treatments (Table 1). The Under treatment had lower (P ≤ 0.05) PWM than the Over treatment, with the Equal treatment being intermediate and not different to the other 2 (P > 0.05; Table 1). In conclusion, reducing litter size after cross-fostering to two piglets below the number of functional teats of the sow decreased PWM and tended to increase weaning weights.
PSIII-3 Effect of Type of Desiccant Used to Dry Piglets at Birth on Postnatal Changes in Rectal Temperature
Authors: Espinal Alicia Olivo , Willard Naomi C , Vande Pol Katherine D , Moest Nicole K , Ellis Michael
Published: May 2021

All piglets experience hypothermia immediately after birth, which can be a predisposing factor for pre-weaning mortality. Drying piglets at birth with a desiccant reduces the extent and duration of postnatal temperature decline. This study compared the effectiveness of different types of commercially-available desiccant products using a CRD with four treatments (applied at birth): Control (piglets not dried) and 3 Desiccant Product treatments [dried with a Mineral-based, Cellulose-based, or Mixed (mineral and cellulose-based) desiccant]. Sows (40) and litters (546 piglets) were randomly allotted to a treatment at the birth of the first piglet. Sows were housed in individual farrowing crates within pens; a heat lamp was suspended over one side of each pen. Room temperature was set at 22.8°C throughout farrowing. Piglets were weighed at birth, those on the Desiccant Product treatments were coated with desiccant until completely dry, and then returned to the pen. Piglet rectal temperatures were measured at 0, 15, 30, 45, 60 and 120 min after birth. Data were analyzed using PROC MIXED of SAS (SAS Inst. Inc., Cary, NC). The model included the fixed effects of treatment, measurement time (repeated measure), and the interaction. There was no effect (P > 0.05) of treatment on rectal temperatures at birth. At all other measurement times, piglets on the Control treatment had lower (P < 0.05) rectal temperatures than those on the 3 Desiccant Product treatments, which had similar (P > 0.05) rectal temperatures. These results suggest that the 3 commercial desiccant products evaluated were equally effective at minimizing the extent and duration of piglet rectal temperature decline in the early postnatal period.
PSIII-8 Genetic Parameter Estimates for Bull Prolificacy and Its Relationship with Scrotal Circumference in a Commercial Beef Cattle Population
Authors: Russell Chad A , Pollak E J , Spangler Matthew L
Published: May 2021

The commercial beef cattle industry relies heavily on the use of natural service sires. Either due to the size of breeding herds or to safe-guard against injury during the breeding season, multiple-sire breeding pastures are utilized. Although each bull might be given an equal opportunity to produce offspring, evidence suggest that there is substantial variation in the number of calves sired by each bull in a breeding pasture. DNA-based paternity assignment enables correct assignment of calves to their respective sires in multi-sire pastures and presents an opportunity to investigate the degree to which this trait complex is under genetic control. Field data from a large commercial ranch were used to estimate genetic parameters for calf count (CC; n=623) and yearling scrotal circumference (SC; n=1962) using univariate and bivariate animal models. Average CC and SC were 12.1±11.1 calves and 35.4±2.30 cm, respectively. Average number breeding seasons per bull and bulls per contemporary group were 1.40 and 24.9, respectively. The model for CC included fixed effects of age during the breeding season (in years) and contemporary group (concatenation of breeding pasture and year). Random effects included additive genetic and permanent environmental effects, and a residual. The model for SC included fixed effects of age (in days) and contemporary group (concatenation of month and year of measurement). Random effects included an additive genetic effect and a residual. Univariate model heritability estimates for CC and SC were 0.237±0.156 and 0.456±0.072, respectively. Similarly, the bivariate model resulted in heritability estimates for CC and SC of 0.240±0.155 and 0.461±0.072, respectively. Repeatability estimates for CC from univariate and bivariate models were 0.517±0.054 and 0.518±0.053, respectively. The estimate of genetic correlation between CC and SC was 0.270±0.220. Parameter estimates suggest that both CC and SC would respond favorably to selection and that CC is moderately repeatable.
PSII-21 The Effects of Lactobacillus Fermentum on Nursery Pig Performance
Authors: Knapp Josh K , Bartenslager Alison , Winkel Shana M , Trenhaile-Grannemann Melanie D , San Andres Joice V , Nguyen Khang , Wittler J’Nan , Kinstler Sydney , Fernando Samodha , Miller Phillip , Burkey Thomas
Published: May 2021

The use of probiotics may be an effective strategy in sustainable pig production. The objective of this experiment was to examine the effects of Lactobacillus fermentum (LF) on nursery pig performance. Weaned pigs (n = 70; average initial BW 6.464 kg) were randomly allotted to 1 of 3 treatments, forming 6 replicates per treatment. Treatments included: 1) pigs fed a standard nursery diet (NRC 2012); CTL; 2) pigs fed CTL with the addition of 10^10 CFU LF per day for phase 1 (wk 2 and 3; LF1); and 3) pigs fed CTL with the addition of 10^10 CFU LF per day for phase 1 and phase 2 (wk 2 thru 5). A common diet was fed during adaptation (wk 1) and during a wash out phase (wk 6). Individual piglet BW were recorded weekly along with pen feed disappearance. No differences were observed in BW by the end of phase 1; at the end of phase 2 the pigs fed LF had a lower BW than the others (19.38, 19.22, 17.3 kg; P = 0.031). This was associated with a reduced feed efficiency seen in week 5 (0.679, 0.656, 0.445; P = 0.0404). However, no differences (P > 0.10) were observed in BW of the pigs throughout the trial and final BW were 24.15, 24.45, 24.3 kg. No differences were observed in ADFI between treatments (ADFI Phase 1: 0.359, 0.366, 0.353 kg/d;P = 0.965; Phase 2: 0.906, 0.896, 0.843 kg/d; P = 0.578; Phase 3: 1.183, 1.227, 1.196 kg; P = 0.920). Results suggest that pigs fed diets supplemented with Lactobacillus fermentum performed similar to pigs fed a conventional diet.
PSII-20 Energy Evaluation of Heat-treated and Intact Extruded-expelled Soybean Meal for Growing Pigs
Authors: Koo Bonjin , Adeshakin Olumide , Nyachoti Martin
Published: May 2021

An experiment was performed to evaluate the energy contents of extruded-expelled soybean meal (EESBM) and the effects of thermal treatment on energy utilization in growing pigs. Eighteen growing barrows (18.03 ± 0.61 kg initial body weight) were individually housed in metabolism crates and randomly allotted to one of three dietary treatments to give six replicates per treatment. The three experimental diets were: a corn-soybean meal-based basal diet and two test diets with simple substitution of a basal diet with intact EESBM or heat-treated EESBM in a 70:30 ratio. Intact EESBM was autoclaved at 120°C for 60 mins to make heat-treated EESBM (heat-EESBM). Pigs were fed the experimental diets for 16 d, including 10 d for adaptation and 6 d for total collection of feces and urine. Pigs were then moved into indirect calorimetry chambers to determine 24-h heat production and 12-h fasting heat production. The energy contents of the tested DESBM were calculated by using the difference method. All data were analyzed using the Mixed procedure of SAS with the individual pig as the experimental unit. Pigs fed heat-EESBM diets showed lower (P < 0.05) apparent total tract digestibility of dry matter (DM), gross energy, and nitrogen than those fed intact EESBM. A trend (P < 0.10) was observed for greater heat increments in pigs fed intact EESBM than those fed heat-EESBM. This resulted in intact EESBM having greater (P < 0.05) digestible energy (DE) and metabolizable energy (ME) contents than heat-EESBM but comparable net energy contents between intact and heat-EESBM. In conclusion, respective values of DE, ME, and net energy are 4,591 kcal/kg, 4,099 kcal/kg, and 3,242 kcal/kg on a DM basis. However, thermal damage during EESBM production should be considered in terms of DE and ME content of EESBM fed to growing pigs.
PSII-16 Effects of Oligosaccharide-based Polymer on Blood Profiles in Weanling Pigs Experimentally Infected with a Pathogenic E. coli
Authors: Kim Kwangwook , He Yijie , Jinno Cynthia , Yang Seijoo , Li Xunde , Bravo David , Cox Eric , Liu Yanhong
Published: May 2021

The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Blood samples were collected before E. coli inoculation (d 0), and on d 2, 5, 8, and 11 post-inoculation (PI). Total and differential blood cell count were analyzed by CBC test. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Supplementation of oligosaccharide-based polymer linearly (P < 0.05) reduced white blood cell counts, neutrophils, eosinophils, and basophils on d 2 PI, and neutrophils on d 5 PI, compared with the control. No differences were observed in total and differential white blood cell counts among AGP and two oligosaccharide-based polymer treatments except that pigs fed with AGP had greater (P < 0.05) lymphocytes on d 2 PI compared with pigs fed with oligosaccharide-based polymer diets. Supplementation of low dose oligosaccharide-based polymer or AGP reduced (P < 0.05) red blood cell count and packed cell volume on d 2 PI, whereas inclusion of high dose oligosaccharide-based polymer or AGP reduced (P < 0.05) packed cell volume on d 5 PI, compared with the control. In conclusion, supplementation of oligosaccharide-based polymer may alleviate the systemic inflammation caused by F18 E. coli infection.
PSIII-9 The Effect of Sire Line on Reproductive Performance During Lactation
Authors: Manu H , Fletcher M , Fang K , Junior Sudario Roberto Silva , Dunkelberger J , Boler D , Eggert J , Baidoo S K
Published: May 2021

The objective of this study was to estimate the effect of sire line on sow and piglet performance during lactation. Commercial Landrace x Large White females (n = 60) were bred to one of the following sire lines: a purebred Duroc line (Line S1), a synthetic (Line S2), or purebred Duroc (Line S3) from a different genetic source. Matings were balanced by line and parity. Females were fed a common gestation and lactation diet during these respective phases. Sow and piglet data were analyzed as a completely randomized design using the GLM procedure of SAS. Results show that, during lactation, females bred to line S3 lost significantly (P = 0.01) more BW (-16.93 ± 3.56 kg) than females bred to line S2 (-2.00 ± 3.50 kg). However, no evidence of a difference (P = 0.18) in BW loss was detected between females bred to line S3 (-16.93 ± 3.56 kg) vs. Line S1 (-7.90 ± 3.56 kg). Further, sows bred to line S3 weaned significantly (P = 0.03) more piglets (12.0 ± 0.28) than females bred to line S2 (10.9 ± 0.27), but not (P = 0.1) compared to females bred to line S1 (11.7 ± 0.27). No significant effect of line (P > 0.05) or parity (P > 0.05) on total number of piglet’s born, number born alive, number of stillborn piglets, or preweaning mortality was detected. The number of mummified fetus tended to be lower in line S2 (0.17 vs 0.66; P = 0.079) and line S3 (0.12 vs 0.66; P = 0.051) sows compared with line S1 sows, respectively. In conclusion, sire line did not have a significant effect on total number born, number born alive, number stillborn, or preweaning mortality. However, females bred to line S3 lost significantly more BW during lact but weaned significantly more piglets than females bred to line S2.
PSIII-13 Effects of Saccharomyces Cerevisiae Fermentation Products on Lactating Sow Performance
Authors: Garcia Ricardo M , Thayer Morgan T , Mills Kayla M , Richert Jacob A , Sharp Katharine G , Rulon Karissa N , Petry Derek B , Robertson Karen , Stewart Kara R , Radcliffe John S , Schinckel Allan P , Richert Brian T
Published: May 2021

Lactating sows (N=140, York x Landrace) were used to evaluate the effects of a liquid prototype (LIQP) and dry (XPC®; Diamond V) Saccharomyces cerevisiae feed additives on sow and litter performance. Sows were fed a common gestation diet (0.55% SID-Lysine) until d112 of pregnancy and then allotted to lactation treatments: 1) Control diet (CON; 1.00% SID-Lysine), 2) CON +15 mL of LIQP from d112 to weaning (LIQ), 3) CON +0.20% of XPC from d112 to weaning (DRY), and 4) DRY +15 mL of LIQP from d112 to d7 post-farrowing (D+L). The LIQP was given once daily using an oral dose gun and XPC was included in the feed. Immunoglobulin concentrations were estimated on colostrum samples using Brix refractometer and piglet d 1 serum immunocrit ratio and plasma IgA and IgG. Daily sow water intake and daily feed intake (DFI) from d112 of gestation to d7 post-farrowing and weekly feed intake (ADFI) were recorded. There were no treatment effects on sow BW, backfat, or loin depth (P >0.05) although sows from LIQ group had numerically smaller BW reductions compared to CON sows (4.9% vs 7.2%, P=0.19). LIQ sows had greater DFI and CON lower DFI during week 1 of lactation (P=0.04) as well as ADFI for weeks 2, 3, and overall lactation period (P< 0.01) with DRY and D+L sows being intermediate. Water intake, immunoglobulins, and litter performance did not differ among treatments (P >0.05) although pigs from LIQ sows, compared to CON, had numerically increased weaning (6.14 vs 5.82kg, P >0.05) and litter weights (63.2 vs 60.1kg, P >0.05). LIQ sows had 0.7 d reduced wean-to-estrus interval (P< 0.001) and tended to have greater conception rates (P=0.07). In conclusion, LIQ supplementation of lactating sows improves feed intake, allowing sows to keep body reserves, and have better subsequent rebreeding performance.
PSIII-14 Hybrid Rye Can Replace Corn in Diets for Growing Pigs Without Impacting Growth Performance Although Pigs Prefer to Eat Corn Rather Than Hybrid Rye If Given a Choice
Authors: McGhee Molly L , Stein Hans H
Published: May 2021

Two experiments were conducted to test the hypotheses that feed preference and growth performance will not be affected if hybrid rye replaces corn in diets for growing pigs. In experiment 1, 36 pigs (32.0 ± 1.8 kg) were housed for 8 d with one gilt and one barrow in each pen. Each pen had 2 feeders containing a corn-based or a hybrid rye-based diet. Feeder positions were switched daily, and feed allotments and disappearances were recorded daily. In experiment 2, 128 pigs (27.2 ± 2.2 kg) were allotted to 32 pens with 4 pigs/pen and 8 replicate pens per diet. A corn-based basal diet and 3 diets containing 22, 45, or 67% hybrid rye were fed for 27 d. Body weights were determined at the start and conclusion of the experiment. Experiment 1 data were analyzed by a paired t-test and experiment 2 data were analyzed using SAS Proc Mixed with diet as the fixed effect and pen as a random effect. Contrast statements were used to test linear and quadratic effects of including graded levels of hybrid rye in diets. In experiment 1, preference for the hybrid rye diet was less (P < 0.05) than for the corn diet on each day and for the overall experiment (Table 1). In experiment 2, body weights of pigs, average daily gain (ADG), and gain:feed did not differ among treatments. Average daily feed intake tended to decrease (linear, P < 0.10) with increased hybrid rye inclusion. Taste preference and satiating effects of dietary fiber in the gastrointestinal tract may contribute to the reduced consumption of hybrid rye in both experiments. Nevertheless, the observation that diet did not influence ADG or gain:feed indicate that growing pigs may be fed diets with high inclusion rates of hybrid rye without negatively impacting growth.
PSIII-16 Growth Performance of Weaned Pigs Fed Barley Differing in Fermentable Starch and Fiber Profile
Authors: Zannatta Joaquin J Sanchez , Wang L F , Beltranena Eduardo , Beattie Aaron D , Newkirk Rex N , Zijlstra Ruurd T
Published: May 2021

Barley grain containing more fermentable starch or fiber might be an attractive energy source in weaned pig diets due to benefits on gut health. Barley rapidly-fermentable carbohydrates may serve as prebiotic and slowly-fermentable fiber may decrease diarrhea in weaned pigs. Steam-explosion processing may disrupt the fiber matrix of hulls, increasing slowly-fermentable fiber of barley. To explore, 220 pigs were fed 1 of 5 diets containing 60% cereal grain: 1) low-fermentable hulled barley (LFB); 2) LFB steam-exploded (LFB-E; 1.2 MPa, 120 s); 3) high β-glucan (10% DM) hull-less barley (HFB); 4) high amylose (17% DM) hull-less barley (HFA); or 5) low-fermentable wheat (LFW). Diets were fed starting 1-week post-weaning and formulated to provide 2.4 and 2.3 Mcal net energy (NE)/kg, 5.5 and 5.1 g standardized ileal digestible lysine/Mcal NE for phase 1 (day 1–14) and phase 2 (day 15–35), respectively. For the entire trial (day 1–35), average daily feed intake (ADFI) and average daily gain (ADG) of pigs did not differ among diets. Gain:feed (G:F) did not differ between LFB and LFW diets, but steam-explosion of hulled barley reduced (P < 0.05) G:F. Feces consistency did not differ between LFB and LFW diets, but was better (P < 0.05) for LFB than HFB, HFA and LFB-E diets. For phase 1, G:F of pigs was lower (P < 0.05) for LFB-E diet than LFW diet. For days 22–28, LFB-E diet had greater (P < 0.01) ADFI than HFA diet and tended (P = 0.09) to have a greater ADG than HFB diet. In conclusion, hulled or hull-less barley grain replaced wheat grain without affecting growth performance in weaned pigs. Hulled barley increased feces consistency. Steam-explosion of hulled barley did not increase growth performance of weaned pigs. Barley grain is an attractive energy source for weaned pigs for managing growth and feces consistency.
PSIII-12 In vitro Evaluation of Purified Fiber Sources for Production of Short-chain Fatty Acids Using Pig Cecal Content as an Inoculum
Authors: Padilla Gabriela E Martinez , Jha Rajesh , Fellner Vivek , van Heugten Eric
Published: May 2021

This study evaluated short-chain fatty acid (SCFA) production from purified fiber sources when fermented in vitro using pig cecal contents as an inoculum. Fiber sources of interest were inulin from chicory root (native and long-chain inulin with 90 and 98% fiber, respectively), pectin from citrus peel (high methoxyl pectin), resistant starch (native starch), potato starch (commercial grade), and β-glucan (β-1,3;β-1,6 yeast-derived). Cellulose and cornstarch were used as indigestible and highly digestible carbohydrates, respectively. Triplicate samples of substrates (2 g) were subjected to enzymatic hydrolysis with pepsin and pancreatin for 6 h. Subsequently, hydrolyzed residues (200 mg) were incubated under anaerobic conditions at 39°C with 30 mL solution of cecal inoculum collected from 3 sows fed a standard commercial diet and buffered mineral solution. After 48 h of incubation, solutions from fermented samples were analyzed for pH, SCFA, and branched-chain fatty acids (BCFA) using gas-liquid chromatography. Enzymatic hydrolysis had no effect on digestion of β-glucan, but total SCFA concentration after fermentation was highest (26.13 mmol/g) followed by resistant starch (22.61 mmol/g) and potato starch (22.20 mmol/g) and was lowest for cellulose (13.91 mmol/g). In contrast, native inulin was highly digested during enzymatic hydrolysis, resulting in the lowest substrate available for fermentation (11.84% DM) and the highest pH (5.98). Enzymatic hydrolysis and fermentation of resistant starch increased (P< 0.001) concentrations of acetate (0.60 mg/g), whereas potato starch and β-glucan yielded more butyrate (0.60 and 0.54 mg/g respectively), and β-glucan resulted in greater (P< 0.001) propionate concentrations (0.69 mg/g). Pectin resulted in the highest fermentation (82.38% DM disappearance) and the lowest pH (4.03) compared to the other fiber sources (P< 0.001) and yielded the lowest BCFA concentration (1.89 mM, P< 0.001). Results suggest that fermentation of resistant starch, potato starch, and β-glucan produced higher SCFA concentrations, while pectin resulted in a decreased pH of fermentation solution.
PSIV-19 Flavors and Sweeteners in Nursery Pig Diets Increased Intake and Reduced Soft Feces in the First Weeks After Weaning
Authors: Perez-Palencia Jorge Y , Chen Xixi , Lv Jirong , Resende Maíra , Chaves Rhuan F , Zeamer Katelyn , Clizer David A , Samuel Ryan S , Levesque Crystal L
Published: May 2021

The stressful events associated with weaning predispose piglets to reduced feed intake, which in concert with immature digestive and immune systems, results in increased incidences of diarrhea and poor performance. Flavors and sweeteners can be used in diets for young pigs as a means of attracting them to dry feed and increase their feed intake and performance after weaning. The objective of this study was to evaluate the effects of dietary inclusion of flavors and sweeteners and their combination on growth performance and post-weaning gut health of nursery pigs. A total of 1,144 weaned pigs (initial BW 6.61 ± 0.2 kg) were used in a randomized complete block design and assigned to one of four dietary treatments, with 11 replicates (pens) of 26 piglets per pen. Dietary treatments were 1) Control: standard 4-phase nursery feeding program; 2) Sweetener: control plus sweetener (0.015%); 3) Flavor: control plus flavoring (0.05%); 4) Combination: control plus sweetener and flavoring. Experimental diets were provided at a budget of 0.9, 3.4, 4.5, and 24 kg/pig in Phase 1, 2, 3, and 4, respectively. A common phase 5 diet was provided ad libitum until d56 after weaning. Feed disappearance and body weight were measured every other week. A pen fecal score assessment was performed at d4, 7, 10, and 14 after weaning. Overall, there were no differences among dietary treatments for growth performance. However, during the first two weeks post-weaning pigs fed diets containing flavors and sweeteners had a greater (P = 0.097) ADFI than control pigs. On d14, pigs fed diets containing flavors and sweeteners had less incidence of soft and watery feces (χ2 < 0.05) compared to control pigs. This study provides evidence that dietary inclusion of flavors and sweeteners has important benefits to the overall health of nursery pigs likely related to feed intake in the period immediately after weaning.