bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Convolutional Neural Networks for Diagnosis of Eosinophilic Esophagitis Based on Endoscopic Imaging
Authors: Okimoto Eiko , Ishimura Norihisa , Adachi Kyoichi , Kinoshita Yoshikazu , Ishihara Shunji , Tada Tomohiro
Published: April 2022

Subjective symptoms associated with eosinophilic esophagitis (EoE), such as dysphagia, are not specific, thus the endoscopic identification of suggestive EoE findings is quite important for facilitating endoscopic biopsy sampling. However, poor inter-observer agreement among endoscopists regarding diagnosis has become a complicated issue, especially with inexperienced practitioners. Therefore, we constructed a computer-assisted diagnosis (CAD) system using a convolutional neural network (CNN) and evaluated its performance as a diagnostic utility. A CNN-based CAD system was developed based on ResNet50 architecture. The CNN was trained using a total of 1192 characteristic endoscopic images of 108 patients histologically proven to be in an active phase of EoE (≥15 eosinophils per high power field) as well as 1192 normal esophagus images. To evaluate diagnostic accuracy, an independent test set of 756 endoscopic images from 35 patients with EoE and 96 subjects with a normal esophagus was examined with the constructed CNN. The CNN correctly diagnosed EoE in 94.7% using a diagnosis per image analysis, with an overall sensitivity of 90.8% and specificity of 96.6%. For each case, the CNN correctly diagnosed 37 of 39 EoE cases with overall sensitivity and specificity of 94.9% and 99.0%, respectively. These findings indicate the usefulness of CNN for diagnosing EoE, especially for aiding inexperienced endoscopists during medical check-up screening.
Thermoplastic Composite Materials Approach for More Circular Components: From Monomer to In Situ Polymerization, a Review
Authors: Valente Marco , Rossitti Ilaria , Biblioteca Ilario , Sambucci Matteo
Published: April 2022

To move toward eco-sustainable and circular composites, one of the most effective solutions is to create thermoplastic composites. The strong commitment of world organizations in the field of safeguarding the planet has directed the research of these materials toward production processes with a lower environmental impact and a strong propensity to recycle the polymeric part. Under its chemical properties, Nylon 6 is the polymer that best satisfies this specific trade-off. The most common production processes that use a thermosetting matrix are described. Subsequently, the work aimed at investigating the use of thermoplastics in the same processes to obtain comparable performances with the materials that are currently used. Particular attention was given to the in situ anionic polymerization process of Nylon 6, starting from the ε-caprolactam monomer. The dependencies of the process parameters, such as temperature, time, pressure, humidity, and concentration of initiators and activators, were therefore investigated with reference to the vacuum infusion technique, currently optimized only to produce thermosetting matrix composites, but promising for the realization of thermoplastic matrix composite; this is the reason why we chose to focus our attention on the vacuum infusion. Finally, three production processes of the polymeric matrix and glass fiber composites were compared in terms of carbon footprint and cumulative energy demand (CED) through life-cycle assessment (LCA).
Design of a Naturally Dyed and Waterproof Biotechnological Leather from Reconstituted Cellulose
Authors: da Silva Junior Claudio José Galdino , de Amorim Julia Didier Pedrosa , de Medeiros Alexandre D’Lamare Maia , de Holanda Cavalcanti Anantcha Karla Lafaiete , do Nascimento Helenise Almeida , Henrique Mariana Alves , do Nascimento Maranhão Leonardo José Costa , Vinhas Glória Maria , de Oliveira Souto Silva Késia Karina , de Santana Costa Andréa Fernanda , Sarubbo Leonie Asfora
Published: April 2022

Consumerism in fashion involves the excessive consumption of garments in modern capitalist societies due to the expansion of globalisation, especially at the beginning of the 21st Century. The involvement of new designers in the garment industry has assisted in creating a desire for new trends. However, the fast pace of transitions between collections has made fashion increasingly frivolous and capable of generating considerable interest in new products, accompanied by an increase in the discarding of fabrics. Thus, studies have been conducted on developing sustainable textile materials for use in the fashion industry. The aim of the present study was to evaluate the potential of a vegan leather produced with a dyed, waterproof biopolymer made of reconstituted bacterial cellulose (BC). The dying process involved using plant-based natural dyes extracted from Allium cepa L., Punica granatum, and Eucalyptus globulus L. The BC films were then shredded and reconstituted to produce uniform surfaces with a constant thickness of 0.10 cm throughout the entire area. The films were waterproofed using the essential oil from Melaleuca alternifolia and wax from Copernicia prunifera. The characteristics of the biotechnological vegan leather were analysed using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexibility and mechanical tests, as well as the determination of the water contact angle (°) and sorption index (s). The results confirmed that the biomaterial has high tensile strength (maximum: 247.21 ± 16.52 N) and high flexibility; it can be folded more than 100 times at the same point without breaking or cracking. The water contact angle was 83.96°, indicating a small water interaction on the biotextile. The results of the present study demonstrate the potential of BC for the development of novel, durable, vegan, waterproof fashion products.
Myocardial Fitness of Bicuspid Aortic Valve Athletes during COVID 19 Pandemic
Authors: Orlandi Melissa , Corsi Marco , Bini Vittorio , De Simone Luciano , Stefani Laura
Published: April 2022

COVID 19 pandemic has induced a large sedentarism in several kinds of sports. Some peculiar categories of athletes could particularly suffer from a prolonged inactivity as those affected by minimal cardiopathies as bicuspid aortic valve (BAV) athletes. This study aims to verify the myocardial performance in a restricted group of BAV athletes compared to a control group of agonistic athletes evaluated by traditional echocardiography and deformation parameters. 2D standard and deformations parameters were measured at rest conditions in BAV athletes and controls. Particularly EF, LVDD/LVS diameters, GLS rotation and twisting were considered as myocardial performance data; E/A, E1 and A1 as diastolic ones. All the 2D standard parameters measured were within the normal range in both groups, especially the EF value. Significant differences were found in the diastolic function with reduced values of E and E1 waves in BAV vs. controls. The strain analysis showed a significant reduction in GLS measured in 2C, 3C, 4C in BAV if compared to controls, while no significant differences were found in torsional and rotational parameters. These results are suggestive for a potential long term negative impact of inactivity on cardiac performance more evident in BAV athletes, if compared to athletes with normal aortic valve. GLS of LV and RV can be considered as a predictive parameter of this mild dysfunction and assumed as follow-up parameter to restore a progressive training.
Characterization of the Morphological Behavior of a Sand Spit Using UAVs
Authors: Paladio-Hernandez Alejandro , Salles Paulo , Arriaga Jaime , López-González José
Published: April 2022

Sand spits are common in wave-dominated environments; with enough sand supply, they can evolve to affect circulation and navigation in channels or inlets. The focus of this paper is on the navigation channel of the Sisal Port, located on the northwestern Yucatan Peninsula (YP) coast, where a sand spit grew and was monitored from its formation (June 2018) until navigation was practically blocked (November 2018). The YP coast is characterized as being microtidal, with significant wave heights ranging from 0.1 to 0.4 m (April to September), and in the presence of high energy events (cold fronts and storms), waves can reach heights of up to 2.5 m offshore at 10 m depth (October to February). Prior to the beginning of UAV surveys, we used photos (June–July 2018) from a stationary field camera and hydrodynamic data from models (WaveWatch III for waves and MARV software for tidal levels) to generate a qualitative description of the sand spit in the channel. Combining products from UAVs flights (DEMs) and hydrodynamic measurements (wave energy flux), we characterized the behavior and response of the sand spit, from its formation near the jetty head, through its consolidation in October 2018, to when a cold front with HS ∼2.5 m breached it in mid-November. The results show that spit formation takes place during calm conditions (e.g., periods dominated by sea breezes), and depending on the energy threshold of high energetic events, this new spit will consolidate or be breached. Migration of the spit is related to overwash events and changes in wave direction. The presented methodology provides a well-rounded tool for characterizing the morphological behavior of spits on a shallow coast, which can be useful for improving coastal management.
Maximum Power Control Algorithm for Power Take-Off System Based on Hydraulic System for Floating Wave Energy Converters
Authors: Roh Chan
Published: April 2022

In this study, a hydraulic system generator power converter was modeled to verify the performance of a hydraulic-based power take-off (PTO) system. Moreover, the characteristics and output performance of the PTO system were analyzed with various load control algorithms applied for maximum power control. The simulation performance was verified through a comparison with actual sea test results. Unlike previous studies on hydraulic-based PTO system control for input power performance, the performance of a hydraulic-based PTO system was analyzed through electrical load control in this study. The electrical load control was analyzed by applying a speed control algorithm based on the perturb and observe algorithm and an optimal torque control algorithm. A load control algorithm suitable for maximum power control of the PTO system was proposed by analyzing the characteristics and power generation performance of the system according to the control variables of each algorithm. The proposed optimal torque control algorithm proved to be suitable for maximum power control of the considered PTO system.
Detection of the Sinking State of Liquid Oil in Breaking Waves Based on Synthesized Data: A Behavior Process Study of Sunken and Submerged Oil
Authors: Fang Shibiao , Mu Lin , Liu Kuan , Liu Darong
Published: April 2022

In computer vision, pollutant detection is a highly concerning issue, and it has been widely used in the fields of pollutant identification, tracking, and precise positioning. In the ocean, oil tends to disperse into the water column as droplets under breaking waves, and it is called sunken and submerged oil. Aiming at the most difficult issue of identifying liquid submerged oil pollution, this paper proposes a method of synthesized data containing specific markers for oil detection. The Canny operator was used to remove the background of the liquid submerged oil. Then, affine transformation was applied to simulate the real situation of oil deformation. Linear mapping was presented by matrix multiplication, and translation was represented by vector addition. At last, bilinear interpolation was used to integrate the oil into the image of the laboratory pictures. In addition, this research randomly added interference information, so that the probability distribution of synthesized data was closer to the probability distribution of the real data. Then, this paper combined various methods to improve the accuracy of liquid oil detection, such as Feature Pyramid Networks, RoIAlign, difficult sample mining. Based on the above methods, 1838 images were synthesized in this paper and combined into a training set. The results show that the average accuracy of the oil detection is increased by 79.72%. The accuracy of the synthesized data method for labeled oil detection was 18.56% higher than that of oil detection without labeling. This research solves the difficulty of obtaining sunken and submerged oil images and the high cost of image annotation.
A Risk-Based Model Using Communication Distance Reduction for the Assessment of Underwater Continuous Noise: An Application to the Bottlenose Dolphin (Tursiops truncatus) Inhabiting the Spanish North Atlantic Marine Demarcation
Authors: Bou-Cabo Manuel , Lara Guillermo , Gutiérrez-Muñoz Paula , Saavedra C. , Miralles Ramón , Espinosa Víctor
Published: April 2022

Over the last decade, national authorities and European administrations have made great efforts to establish methodological standards for the assessment of underwater continuous noise, especially under the requirements set by the Marine Strategy Framework Directive (MSFD). Through the MSFD implementation across EU Member States Marine Reporting Units (MRUs), it is intended to establish the Good Environmental Status (GES) whether it is achieved or not. The evaluation of the Sound Pressure Level (SPL) at the local or regional scale for 1/3 octave band of 63 Hz and 125 Hz and the identification of long temporary trends were considered to be a priority due to the valuable information they can offer in relation to continuous low-frequency noise. Nevertheless, the methodology to determine threshold values from which to evaluate the GES has become difficult to define, and new approaches and considerations are currently being discussed by groups of experts, such as the technical subgroup on underwater acoustics (TGnoise) and regional commissions (e.g., OSPAR). This work presents a methodology to perform the assessment of a given area, providing a risk index that is related to potential appearance of masking effect due to the underwater noise produced by marine traffic. The risk index is hinged on the calculation of area under curves defined by the density of animals and a variable related to underwater noise SPL, defined as percentage of communication distance reduction. At this stage, the methodology presented does not consider physiological or behavioral mechanisms to overcome the masking by animals. The methodology presented has been applied to the bottlenose dolphin (Tursiops truncatus) inhabiting the ABIES—NOR marine demarcation to illustrate the possible use of risk-based models to manage marine areas related to human pressures, such as marine traffic, with the potential adverse impact on a given species (e.g., masking effect).
DS-Based 2-DOF PID Controller of a Regasification System for LNG-Fueled Marine Engine
Authors: So Gun-Baek
Published: April 2022

As international regulations on greenhouse gas emissions are becoming stricter, the development of eco-friendly main engines is required for ocean-going ships. To use cryogenic LNG as a fuel for marine engines, it has to be converted to gas. This processing device is called an LNG regasification system. This paper presents a DS-based PID controller for glycol temperature control of a regasification system for LNG-fueled marine engines. In controller design, linear PID controllers have something of a catch-22 relationship: Fast response requires a large gain, which results in a large overshoot. To solve this problem, a DS-based 2-DOF PID controller is considered, which consists of a PID controller to reject disturbances in regulatory response and a set-point filter to reduce overshoot in servo response. The controller design focuses on improving disturbance rejection performance. The DS method is based on comparing the desired closed-loop characteristic equation with the closed-loop characteristic equation consisting of a control object and PID controller. The proposed controller is applied to the glycol temperature control of the regasification system for LNG-fuel marine engines, and its performance is compared with existing methods to show its effectiveness and applicability.
Development of a Real-Time PCR Assay to Identify and Distinguish between Cryptococcus neoformans and Cryptococcus gattii Species Complexes
Authors: Tay Enoch , Chen Sharon C-A. , Green Wendy , Lopez Ronald , Halliday Catriona L.
Published: April 2022

Cryptococcus neoformans and Cryptococcus gattii are the principle causative agents of cryptococcosis. Differences in epidemiological and clinical features, and also treatment, mean it is important for diagnostic laboratories to distinguish between the two species. Molecular methods are potentially more rapid than culture and cryptococcal antigen (CRAG) detection; however, commercial PCR-based assays that target Cryptococcus do not distinguish between species. Here, we developed a real-time PCR assay targeting the multicopy mitochondrial cytochrome b (cyt b) gene to detect C. neoformans and C. gattii in clinical specimens. Assay performance was compared with culture, histopathology, CRAG and panfungal PCR/DNA sequencing. The cyt b-directed assay accurately detected and identified all eight C. neoformans/gattii genotypes. High-resolution melt curve analysis unambiguously discriminated between the two species. Overall, assay sensitivity (96.4%) compared favorably with panfungal PCR (76.9%) and culture (14.5%); assay specificity was 100%. Of 25 fresh frozen paraffin embedded (FFPE) specimens, assay sensitivity was 96% (76% for panfungal PCR; 68% for histopathology). The Cryptococcus-specific PCR is a rapid (~4 h) sensitive method to diagnose (or exclude) cryptococcosis and differentiate between the two major species. It is suitable for use on diverse clinical specimens and may be the preferred molecular method for FFPE specimens where clinical suspicion of cryptococcosis is high.
Clinical and Microbiological Characteristics of Neonates with Candidemia and Impacts of Therapeutic Strategies on the Outcomes
Authors: Chen Yu-Ning , Hsu Jen-Fu , Chu Shih-Ming , Lai Mei-Yin , Lin Chih , Huang Hsuan-Rong , Yang Peng-Hong , Chiang Ming-Chou , Tsai Ming-Horng
Published: April 2022

Neonatal candidemia is associated with significant morbidities and a high mortality rate. We aimed to investigate the clinical characteristics of Candida bloodstream infections in neonates and the impact of therapeutic strategies on the outcomes. We identified all the neonates with candidemia from a medical center in Taiwan over an 18-year period (2003–2021) and analyzed them. Clinical isolates were confirmed by DNA sequencing, and antifungal susceptibility testing was performed. The prognostic factors associated with clinical treatment failure (30-day, all-cause mortality and persistent candidemia > 72 h after antifungal agents) and in-hospital mortality were analyzed using logistic regression modeling. A total of 123 neonates with 139 episodes of candidemia were included in the study. The median (IQR) gestational age and birth weight of the neonates with candidemia were 29.0 (26.0–35.0) weeks and 1104.0 (762.0–2055) g, respectively. The most common Candida spp. was Candida albicans (n = 57, 41.0%), followed by C. parapsilosis (n = 44, 31.7%), Candida guilliermondii (n = 12, 8.6%), and C. glabrata (n = 11, 7.9%). The overall susceptibility to fluconazole was 81.3%, and the resistant rates against other antifungal agents were less than 3%. The cumulative mortality rate at 7 and 30 days after the first episode of candidemia was 11.3% and 32.3%, respectively. The overall in-hospital mortality rate was 42.3%. The treatment outcomes did not change over the study period and were not affected by delayed initiation of antifungal agents. Multivariate analysis showed that delayed catheter removal (odds ratio [OR], 5.54; 95% confidence interval [CI]: 1.93–15.86, p = 0.001), septic shock (OR, 7.88; 95% CI: 2.83–21.93, p < 0.001), and multiple chronic comorbidities (OR, 8.71; 95% CI: 1.82–41.81, p = 0.007) were independently associated with the final in-hospital mortality. We concluded that the overall mortality of neonatal candidemia has remained consistently high over the past decade. Prompt early catheter removal and an aggressive treatment strategy for neonatal candidemia with septic shock would be critical to improving patient outcomes.
A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats
Authors: Sakima Vinicius Tatsuyuji , Vega-Chacón Yuliana , Cerri Paulo Sergio , Shokeen Bhumika , Lux Renate , Mima Ewerton Garcia de Oliveira
Published: April 2022

Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressions were taken from the rats’ palate to individually fabricate acrylic devices. Mono- and multispecies biofilms of C. albicans, C. glabrata, and C. tropicalis were grown on the devices, which were then cemented on posterior teeth and kept in the rats’ palate for four weeks. Microbial samples from the palate and the device were quantified. Oral microbiome of rats inoculated with C. albicans was analyzed by 16S rRNA gene sequencing. Log10(CFU/mL) were analyzed by mixed or two-way MANOVA (α = 0.05). Candida spp. and acrylic device did not induce palatal inflammation macroscopically nor microscopically. Although there was an increase (p < 0.001) of the total microbiota and female rats demonstrated higher (p = 0.007) recovery of Candida spp. from the palate, the gender differences were not biologically relevant. The microbiome results indicate an increase in inflammatory microbiota and reduction in health-associated micro-organisms. Although Candida spp. and acrylic device did not induce DS in immunocompetent rats, the shift in microbiota may precede manifestation of inflammation.
Radiography and Computed Tomography Detection of Intimal and Medial Calcifications in Leg Arteries in Comparison to Histology
Authors: Vos Annelotte , Vink Aryan , Kockelkoren Remko , Takx Richard A. P. , Celeng Csilla , Mali Willem P. T. M. , Isgum Ivana , Bleys Ronald L. A. W. , de Jong Pim A.
Published: April 2022

Calcifications are common in the tunica intima and tunica media of leg arteries. There is growing interest in medial arterial calcifications, as they may be modifiable with treatment. We aimed to investigate radiography and computed tomography (CT) for the detection and characterization of both types of arterial calcification in leg arteries in relation to histology. In a postmortem study we therefore investigated 24 popliteal and 24 tibial arteries. The reference standard was presence of arterial calcification and the dominance of intimal or medial calcification on histology. Radiographs and CT scans were scored for presence of calcification and for dominant intimal or medial pattern based on prespecified criteria (annularity, thickness, continuity). Both radiography and CT detected 87% of histologically proven calcifications but missed mild calcifications in 13%. When only the arteries with detected calcifications were included, a moderate agreement was observed on intimal/medial location of calcifications between histology and radiography (correct in 19/24 arteries (79%); Kappa 0.58) or CT (correct in 33/46 arterial segments (72%); Kappa 0.48). With both modalities there was a slight tendency to classify intimal calcifications as being located in the media and to miss media calcification. Our study demonstrates the potential and limitations of both radiography and CT to detect and classify arterial calcifications in leg arteries.
An Idealized Clinicogenomic Registry to Engage Underrepresented Populations Using Innovative Technology
Authors: Silva Patrick , Dahlke Deborah Vollmer , Smith Matthew Lee , Charles Wendy , Gomez Jorge , Ory Marcia G. , Ramos Kenneth S.
Published: April 2022

Current best practices in tumor registries provide a glimpse into a limited time frame over the natural history of disease, usually a narrow window around diagnosis and biopsy. This creates challenges meeting public health and healthcare reimbursement policies that increasingly require robust documentation of long-term clinical trajectories, quality of life, and health economics outcomes. These challenges are amplified for underrepresented minority (URM) and other disadvantaged populations, who tend to view the institution of clinical research with skepticism. Participation gaps leave such populations underrepresented in clinical research and, importantly, in policy decisions about treatment choices and reimbursement, thus further augmenting health, social, and economic disparities. Cloud computing, mobile computing, digital ledgers, tokenization, and artificial intelligence technologies are powerful tools that promise to enhance longitudinal patient engagement across the natural history of disease. These tools also promise to enhance engagement by giving participants agency over their data and addressing a major impediment to research participation. This will only occur if these tools are available for use with all patients. Distributed ledger technologies (specifically blockchain) converge these tools and offer a significant element of trust that can be used to engage URM populations more substantively in clinical research. This is a crucial step toward linking composite cohorts for training and optimization of the artificial intelligence tools for enhancing public health in the future. The parameters of an idealized clinical genomic registry are presented.
Correction: Rossignol, D.A.; Frye, R.E. Cerebral Folate Deficiency, Folate Receptor Alpha Autoantibodies and Leucovorin (Folinic Acid) Treatment in Autism Spectrum Disorders: A Systematic Review and Meta-Analysis. J. Pers. Med. 2021, 11, 1141
Authors: Rossignol Daniel A. , Frye Richard E.
Published: April 2022

In the original publication [...]
Whole-Genome Sequencing of 100 Genomes Identifies a Distinctive Genetic Susceptibility Profile of Qatari Patients with Hypertension
Authors: Alsamman Alsamman M. , Almabrazi Hakeem , Zayed Hatem
Published: April 2022

Essential hypertension (EH) is a leading risk condition for cardiovascular and renal complications. While multiple genes are associated with EH, little is known about its genetic etiology. Therefore, this study aimed to screen for variants that are associated with EH in 100 hypertensive/100 control patients comprising Qatari individuals using GWASs of whole-genome sequencing and compare these findings with genetic data obtained from more than 10,000 published peer-reviewed studies on EH. The GWAS analysis performed with 21,096 SNPs revealed 38 SNPs with a significant ≥4 log-p value association with EH. The two highest EH-associated SNPs (rs921932379 and rs113688672) revealed a significance score of ≥5 log-p value. These SNPs are located within the inter-genic region of GMPS-SETP14 and ISCA1P6-AC012451.1, respectively. Text mining yielded 3748 genes and 3078 SNPs, where 51 genes and 24 SNPs were mentioned in more than 30 and 10 different articles, respectively. Comparing our GWAS results to previously published articles revealed 194 that are unique to our patient cohort; of these, 13 genes that have 26 SNPs are the most significant with ≥4 log-p value. Of these genes, C2orf47-SPATS2L contains nine EH-associated SNPs. Most of EH-associated genes are related to ion gate channel activity and cardiac conduction. The disease–gene analysis revealed that a large number of EH-associated genes are associated with a variety of cardiovascular disorders. The clustering analysis using EH-associated SNPs across different ethnic groups showed high frequency for the minor allele in different ethnic groups, including Africans, East Asians, and South Asians. The combination of GWAS and text mining helped in identifying the unique genetic susceptibility profile of Qatari patients with EH. To our knowledge, this is the first small study that searched for genetic factors associated with EH in Qatari patients.
Assessment of Dynamic Changes in Stressed Volume and Venous Return during Hyperdynamic Septic Shock
Authors: Chalkias Athanasios , Laou Eleni , Papagiannakis Nikolaos , Spyropoulos Vaios , Kouskouni Evaggelia , Theodoraki Kassiani , Xanthos Theodoros
Published: April 2022

The present work investigated the dynamic changes in stressed volume (Vs) and other determinants of venous return using a porcine model of hyperdynamic septic shock. Septicemia was induced in 10 anesthetized swine, and fluid challenges were started after the diagnosis of sepsis-induced arterial hypotension and/or tissue hypoperfusion. Norepinephrine infusion targeting a mean arterial pressure (MAP) of 65 mmHg was started after three consecutive fluid challenges. After septic shock was confirmed, norepinephrine infusion was discontinued, and the animals were left untreated until cardiac arrest occurred. Baseline Vs decreased by 7% for each mmHg decrease in MAP during progression of septic shock. Mean circulatory filling pressure (Pmcf) analogue (Pmca), right atrial pressure, resistance to venous return, and efficiency of the heart decreased with time (p < 0.001 for all). Fluid challenges did not improve hemodynamics, but noradrenaline increased Vs from 107 mL to 257 mL (140%) and MAP from 45 mmHg to 66 mmHg (47%). Baseline Pmca and post-cardiac arrest Pmcf did not differ significantly (14.3 ± 1.23 mmHg vs. 14.75 ± 1.5 mmHg, p = 0.24), but the difference between pre-arrest Pmca and post-cardiac arrest Pmcf was statistically significant (9.5 ± 0.57 mmHg vs. 14.75 ± 1.5 mmHg, p < 0.001). In conclusion, the baseline Vs decreased by 7% for each mmHg decrease in MAP during progression of hyperdynamic septic shock. Significant changes were also observed in other determinants of venous return. A new physiological intravascular volume existing at zero transmural distending pressure was identified, termed as the rest volume (Vr).
Vague Pension Future: Empirical Evidence from the Israeli Radical Privatized Market
Authors: Wolf Ishay , Levi Smadar
Published: April 2022

We examine the future benefits of the Israeli privatized pension system, which is considered as a model of transition to funded pension systems worldwide. This research is based on an extensive database obtained from one of the largest traditional private funds in the market. The results paint a concerning picture regarding the adequacy of benefits and quality of life in old age. Israel’s radical privatized pension model signals a warning to other nations. We show that, even with high returns, most individuals cannot handle the magnitude of financial and labor risks accumulated during their career and retirement. We recommend more balanced government intervention as well as the use of risk-sharing mechanisms such as providing minimum pension guarantee and strengthening the unfunded social security pillar.
AbundanceR: A Novel Method for Estimating Wildlife Abundance Based on Distance Sampling and Species Distribution Models
Authors: Li Xinhai , Li Ning , Li Baidu , Sun Yuehua , Gao Erhu
Published: April 2022

Appropriate field survey methods and robust modeling approaches play an important role in wildlife protection and habitat management because reliable information on wildlife distribution and abundance is important for conservation planning and actions. However, accurately estimating animal abundance is challenging in most species, as usually only a small proportion of the population can be detected during surveys. Species distribution models can predict the habitat suitability index, which differs from species abundance. We designed a method to adjust the results from species distribution models to achieve better accuracy for abundance estimation. This method comprises four steps: (1) conducting distance sampling, recording species occurrences, and surveying routes; (2) performing species distribution modeling using occurrence records and predicting animal abundance in each quadrat in the study area; (3) comparing the difference between field survey results and predicted abundance in quadrats along survey routes, adjusting model prediction, and summing up to obtain total abundance in the study area; (4) calculating uncertainty from three sources, i.e., distance sampling (using detection rate), species distribution models (using R squared), and differences between the field survey and model prediction [using the standard deviation of the ratio (observation/prediction) at different zones]. We developed an R package called abundanceR to estimate wildlife abundance and provided data for the Tibetan wild ass (Equus kiang) based on field surveys at the Three-River-Source National Park, as well as 29 layers of environmental variables covering the terrestrial areas of the planet. Our method can provide accurate estimation of abundance for animals inhabiting open areas that can be easily observed during distance sampling, and whose spatial heterogeneity of animal density within the study area can be accurately predicted using species distribution models.
Could Lavender Farming Go from a Niche Crop to a Suitable Solution for Romanian Small Farms?
Authors: Vijulie Iuliana , Lequeux-Dincă Ana-Irina , Preda Mihaela , Mareci Alina , Matei Elena
Published: April 2022

Lavender crops have had an impressive continuous development in recent years, being currently a suitable alternative to other traditional crops because they can yield a high profit per hectare. This can be especially useful in Romania, with its high prevalence of subsistence and semi-subsistence farms. This study aims to analyse the issue of small emergent lavender farms in the context of the current Romanian agricultural background, including the framework mechanisms for implementing the Common Agricultural Policy at a national level. The research uses the qualitative survey method to provide broad, synthetic, analytical insights into small lavender farms/businesses in Romania, considering the perspective of the following two target groups: farm owners and civil servants with agricultural expertise. The main results show that both sample groups agree that lavender farms can be successful and satisfactory solutions. Increasing participation in information and training sessions may improve farmers’ access to financing mechanisms, but both small farmers and civil servants with agricultural expertise identify a series of problems, mainly regarding the absence of a dedicated market for lavender-based products and a lack of labour force, both essential for maintaining the farming–processing–commercialising chain. The authors also conclude that a more flexible and future harmonisation between Romania’s agricultural realities, the Common Agricultural Policy, and the National Rural Development Programme would improve lavender farming’s social and economic impact. Follow-up research may envisage more in-depth market analyses for this emerging sector in Romania, facing obvious competition, but which could also benefit from good practice exchanges in the region.
Tourism Planning in Underdeveloped Regions—What Has Been Going Wrong? The Case of Extremadura (Spain)
Authors: Leal-Solís Ana , Robina-Ramírez Rafael
Published: April 2022

The article proposes a planning model to develop tourism from a holistic perspective that includes economic, population and social aspects. Following a participatory methodology, the objective of the study is to propose a tourism planning model that contributes to the development of rural territories based on the involvement of tourism planners in the study. A total of 205 tourism planners and 443 tourism offices participated in the study. The data obtained were analysed using the PLS-SEM method. The results indicate that tourism planning has to be carried out by a professional team with diverse technical profiles that monitors the objectives set and adapts them to changes in the environment.
Humanitarian and Compassionate Applications: A Critical Look at Canadian Decision-Makers’ Assessment of Claims from “Vulnerable” Applicants
Authors: Delisle Anthony , Nakache Delphine
Published: April 2022

For many people who have made Canada their home but have uncertain legal status and are ineligible to apply for permanent residence through other channels, the Humanitarian and Compassionate (H&C) application is the only available pathway to permanent residence and stability in Canada. Applications for permanent residence on H&C grounds have become a key component of Canada’s immigration system and yet this pathway remains under-researched. Drawing upon extensive desk research and the preliminary analysis of interview data, this article addresses this gap in the scholarship by offering a critical analysis of the H&C program. In it, we begin by discussing the specific challenges that this highly discretionary decision-making process poses for vulnerable applicants and suggest areas for improvement. We then focus on H&C applications and decisions that directly impact children and explain why a change in the Canadian application of the best interests of the child principle is required. Finally, we consider two recent trends in H&C cases: the sharp increase in the number of applications and the increasingly high rates of refusal. Throughout this analysis, we highlight the negative repercussions the current system has on the most vulnerable categories of migrants and the need to better understand these phenomena.
Photobiomodulation Therapy Combined with Static Magnetic Field Reduces Pain in Patients with Chronic Nonspecific Neck and/or Shoulder Pain: A Randomized, Triple-Blinded, Placebo-Controlled Trial
Authors: Teixeira Adeilson Matias , Leal-Junior Ernesto Cesar Pinto , Casalechi Heliodora Leão , Vanin Adriane Aver , de Paiva Paulo Roberto Vicente , Melo Fernando Hess Câmara , Johnson Douglas Scott , Tomazoni Shaiane Silva
Published: April 2022

Photobiomodulation therapy (PBMT) has been used to treat patients with chronic neck and/or shoulder pain. However, it is unknown whether the concurrent use of PBMT and static magnetic field (PBMT-sMF) also has positive effects in these patients. The aim of this study was to investigate the effects of PBMT-sMF versus placebo on pain intensity, range of motion (ROM) and treatment satisfaction in patients with chronic nonspecific neck and/or shoulder pain. A randomized controlled trial, with blinded assessors, therapists and patients was carried out. Seventy-two patients with chronic nonspecific neck and/or shoulder pain were randomized to either active PBMT-sMF (n = 36) or placebo PBMT-sMF (n = 36). Patients were treated twice weekly, over 3 weeks. Primary outcome was pain intensity, measured 15 min after the last treatment session and at 24-, 48-, 72-h, and 7-days after the last treatment. Secondary outcomes were ROM, patient’ treatment satisfaction, and adverse effects. PBMT-sMF was able to reduce pain intensity in all time points tested compared to placebo (p < 0.05). There was no difference between groups in the secondary outcomes (p > 0.05). Our results suggest that PBMT-sMF is better than placebo to reduce pain in patients with chronic nonspecific neck and/or shoulder pain at short-term.
The Platelet-Derived Growth Factor Pathway in Pulmonary Arterial Hypertension: Still an Interesting Target?
Authors: Solinc Julien , Ribot Jonathan , Soubrier Florent , Pavoine Catherine , Dierick France , Nadaud Sophie
Published: April 2022

The lack of curative options for pulmonary arterial hypertension drives important research to understand the mechanisms underlying this devastating disease. Among the main identified pathways, the platelet-derived growth factor (PDGF) pathway was established to control vascular remodeling and anti-PDGF receptor (PDGFR) drugs were shown to reverse the disease in experimental models. Four different isoforms of PDGF are produced by various cell types in the lung. PDGFs control vascular cells migration, proliferation and survival through binding to their receptors PDGFRα and β. They elicit multiple intracellular signaling pathways which have been particularly studied in pulmonary smooth muscle cells. Activation of the PDGF pathway has been demonstrated both in patients and in pulmonary hypertension (PH) experimental models. Tyrosine kinase inhibitors (TKI) are numerous but without real specificity and Imatinib, one of the most specific, resulted in beneficial effects. However, adverse events and treatment discontinuation discouraged to pursue this therapy. Novel therapeutic strategies are currently under experimental evaluation. For TKI, they include intratracheal drug administration, low dosage or nanoparticles delivery. Specific anti-PDGF and anti-PDGFR molecules can also be designed such as new TKI, soluble receptors, aptamers or oligonucleotides.
Computational Investigations on the Natural Small Molecule as an Inhibitor of Programmed Death Ligand 1 for Cancer Immunotherapy
Authors: Kumar Geethu S , Moustafa Mahmoud , Sahoo Amaresh Kumar , Malý Petr , Bharadwaj Shiv
Published: April 2022

Several therapeutic monoclonal antibodies approved by the FDA are available against the PD-1/PD-L1 (programmed death 1/programmed death ligand 1) immune checkpoint axis, which has been an unprecedented success in cancer treatment. However, existing therapeutics against PD-L1, including small molecule inhibitors, have certain drawbacks such as high cost and drug resistance that challenge the currently available anti-PD-L1 therapy. Therefore, this study presents the screening of 32,552 compounds from the Natural Product Atlas database against PD-L1, including three steps of structure-based virtual screening followed by binding free energy to refine the ideal conformation of potent PD-L1 inhibitors. Subsequently, five natural compounds, i.e., Neoenactin B1, Actinofuranone I, Cosmosporin, Ganocapenoid A, and 3-[3-hydroxy-4-(3-methylbut-2-enyl)phenyl]-5-(4-hydroxybenzyl)-4-methyldihydrofuran-2(3H)-one, were collected based on the ADMET (absorption, distribution, metabolism, excretion, and toxicity) profiling and binding free energy (>−60 kcal/mol) for further computational investigation in comparison to co-crystallized ligand, i.e., JQT inhibitor. Based on interaction mapping, explicit 100 ns molecular dynamics simulation, and end-point binding free energy calculations, the selected natural compounds were marked for substantial stability with PD-L1 via intermolecular interactions (hydrogen and hydrophobic) with essential residues in comparison to the JQT inhibitor. Collectively, the calculated results advocate the selected natural compounds as the putative potent inhibitors of PD-L1 and, therefore, can be considered for further development of PD-L1 immune checkpoint inhibitors in cancer immunotherapy.
Recombinant Mitochondrial Genomes Reveal Recent Interspecific Hybridization between Invasive Salangid Fishes
Authors: Balakirev Evgeniy
Published: April 2022

The interspecific recombination of the mitochondrial (mt) genome, if not an experimental artifact, may result from interbreeding of species with broken reproductive barriers, which, in turn, is a frequent consequence of human activities including species translocations, habitat modifications, and climate change. This issue, however, has not been addressed for Protosalanx chinensis and other commercially important and, simultaneously, invasive salangid fishes that were the product of successful aquaculture in China. To assess the probability of interspecific hybridization, we analyzed the patterns of diversity and recombination in the complete mitochondrial (mt) genomes of these fishes using the GenBank resources. A sliding window analysis revealed a non-uniform distribution of the intraspecific differences in P. chinensis with four highly pronounced peaks of divergence centered at the COI, ND4L-ND4, and ND5 genes, and also at the control region. The corresponding divergent regions in P. chinensis show a high sequence similarity (99–100%) to the related salangid fishes, Neosalanx tangkahkeii and N. anderssoni. This observation suggests that the divergent regions of P. chinensis may represent a recombinant mitochondrial DNA (mtDNA) containing mt genome fragments belonging to different salangid species. Indeed, four, highly significant (pairwise homoplasy index test, P < 0.00001) signals of recombination have been revealed at coordinates closely corresponding to the divergent regions. The recombinant fragments are, however, not fixed, and different mt genomes of P. chinensis are mosaic, containing different numbers of recombinant events. These facts, along with the high similarity or full identity of the recombinant fragments between the donor and the recipient sequences, indicate a recent interspecific hybridization between P. chinensis and two Neosalanx species. Alternative hypotheses, including taxonomical misidentifications, sequence misalignments, DNA contamination, and/or artificial PCR recombinants, are not supported by the data. The recombinant fragments revealed in our study represent diagnostic genetic markers for the identification and distinguishing of hybrids, which can be used to control the invasive dynamics of hybrid salangid fishes.
Learning from the Community to Predict Nutrition Status of Children Aged 6–24 Months in Gulu District, Northern Uganda: A Case Control Study
Authors: Ssenyondo Muzafaru , Bachou Hanifa , Bukenya Richard , Kajjura Richard , Guwatudde David
Published: April 2022

The feeding and caring practices of infants and young children are critical to children’s nutrition status and development milestones. Most nutrition studies have focused on unfavorable factors that contribute to malnutrition rather than favorable factors that promote good nutrition status among children. This study aimed at identifying predictors of normal nutrition status among children aged 6–24 months in Gulu District, Northern Uganda. A matched case-control study was conducted on a sample of 300 (i.e., 100 cases and 200 controls) purposively selected children during October–December 2021. Controls were children that had normal nutrition status, whereas cases with undernourished children had at least one type of undernutrition. Logistic regression was used to determine the predictors of good nutrition status using odds ratios (ORs). The mean age of the cases and controls was 15 months (SD ± 6) and 13 months (SD ± 5), respectively. At multivariable analysis, breastfeeding in the first hour of the child’s life (AOR = 3.31 95% CI. 1.52–7.23), use of family planning (AOR = 2.21 95% CI. 1.25–3.90), number of under-fives in the household (AOR = 0.31 95% CI. 0.13–0.73) and hand washing with soap (AOR = 3.63 95% CI. 1.76–7.49) were significantly independently associated with a child’s good nutrition status. Interventions that can improve children’s nutrition status include breastfeeding in the first hour of child’s life, use of family planning methods, child spacing and hand washing with soap.
A Comprehensive Review on the Risk of Metabolic Syndrome and Cardiovascular Disease after Liver Transplantation
Authors: Chauhan Kashyap , Khan Adnan , Chowdhury Salil , Ross Heather M. , Parra Natalia Salinas , Halegoua-DeMarzio Dina
Published: April 2022

Survival rates after liver transplantation have increased dramatically over the past 20 years. Cardiovascular disease is the most common extra-hepatic cause of mortality in the long-term post liver transplant. This is intimately linked with both the higher pre-existing rates of metabolic syndrome in these patients as well as increased propensity to develop de novo metabolic syndrome post-transplant. This unfavorable metabolic profile that contributes to cardiovascular disease is multifactorial and largely preventable. This review explores metabolic syndrome and cardiovascular disease and their contributory factors post liver transplantation to highlight areas for potential intervention and thus reduce the significant morbidity and mortality of patients due to metabolic syndrome and cardiovascular disease.