bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Equilibrium of CFRP-RC Square Elements under Unequal Lateral Impact
Authors: AL-Bukhaiti Khalil , Yanhui Liu , Shichun Zhao , Abas Hussien , Aoran Dong
Published: June 2021

Building structure regularly needs reinforcement due to damage, specification requirements, and functional changes; carbon fiber reinforced polymer (CFRP) is widely used in structural reinforcement due to its high strength, lightweight, good corrosion resistance and easy construction. The regular square section reinforced concrete frame elements strengthened by CFRP material are taken as the research object. The dynamic response of CFRP to reinforced concrete elements under unequal lateral impact was discussed. This technical paper demonstrates that the test elements are subject to the bending failure mode, and the impact point and the near impact point support are severely damaged areas; the transversely wrapped elements are more abruptly broken, and the longitudinal wrapping elements and the number of wrapping layers can effectively reduce the level of damage. Analysis of the impact, deflection, and strain time history curves obtained in the test show that the wrapping mode and the number of layers have less influence on the impact force peak; the longitudinally wrapped elements and the plateau segment take longer. Dynamic equilibrium principle equation was proposed based on the experimental results. The horizontal partition plateau segment fluctuates greatly; the number of vertical wrap layers increases the plateau value. The larger the number of layers, the smaller the deflection caused by the impact. The longitudinal wrapping can effectively transmit the force.
Shear Band Evolution under Cyclic Loading and Fatigue Property in Metallic Glasses: A Brief Review
Authors: Wang Xiaodi , Wu Shaojie , Qu Ruitao , Zhang Zhefeng
Published: June 2021

The fatigue damage and fracture of metallic glasses (MGs) were reported to be dominated by shear band. While there exist several reviews about the fatigue behavior of MGs, an overview that mainly focuses on shear bands under cyclic loading is urgent, and is of great importance for the understanding of fatigue mechanisms and properties. In this review paper, based on the previous research results, the shear band evolution under cyclic loading including shear band formation, propagation and cracking, was summarized and elucidated. Furthermore, one strategy of enhancing the fatigue property through manipulating the microstructure to suppress the shear band formation was proposed. Additionally, the applications of the effect of annealing treatment and processing condition on fatigue behaviors were utilized to verify the strategy. Finally, several future directions of fatigue research in MG were presented.
Microstructural Evolutions of 2N Grade Pure Al and 4N Grade High-Purity Al during Friction Stir Welding
Authors: Nagira Tomoya , Liu Xiaochao , Ushioda Kohasaku , Fujii Hidetoshi
Published: June 2021

The grain refinement mechanisms along the material flow path in pure and high-purity Al were examined, using the marker insert and tool stop action methods, during the rapid cooling friction stir welding using liquid CO2. In pure Al subjected to a low welding temperature of 0.56Tm (Tm: melting point), the resultant microstructure consisted of a mixture of equiaxed and elongated grains, including the subgrains. Discontinuous dynamic recrystallization (DDRX), continuous dynamic recrystallization (CDRX), and geometric dynamic recrystallization are the potential mechanisms of grain refinement. Increasing the welding temperature and Al purity encouraged dynamic recovery, including dislocation annihilation and rearrangement into subgrains, leading to the acceleration of CDRX and inhibition of DDRX. Both C- and B/B^-type shear textures were developed in microstructures consisting of equiaxed and elongated grains. In addition, DDRX via high-angle boundary bulging resulted in the development of the 45° rotated cube texture. The B/B^ shear texture was strengthened for the fine microstructure, where equiaxed recrystallized grains were fully developed through CDRX. In these cases, the texture is closely related to grain structure development.
Numerical Study of the Micro-Jet Formation in Double Flow Focusing Nozzle Geometry Using Different Water-Alcohol Solutions
Authors: Belšak Grega , Bajt Saša , Šarler Božidar
Published: June 2021

The purpose of this work is to determine, based on the computational model, whether a mixture of a binary liquid is capable of producing longer, thinner and faster gas-focused micro-jets, compared to the mono-constituent liquids of its components. Mixtures of water with two different alcohols, water + ethanol and water + 2-propanol, are considered. The numerical study of pre-mixed liquids is performed in the double flow focusing nozzle geometry used in sample delivery in serial femtosecond crystallography experiments. The study reveals that an optimal mixture for maximizing the jet length exists both in a water + ethanol and in a water + 2-propanol system. Additionally, the use of 2-propanol instead of ethanol results in a 34% jet length increase, while the jet diameters and velocities are similar for both mixtures. Pure ethanol and pure 2-propanol are the optimum liquids to achieve the smallest diameter and the fastest jets. However, the overall aim is to find a mixture with the longest, the smallest and the fastest jet. Based on our simulations, it appears that water + 2-propanol mixture might be slightly better than water + ethanol. This study reveals the dominant effect of liquid viscosity on the jet breakup process in a flow focusing nozzles operated under atmospheric conditions.
Numerical Valuation of American Basket Options via Partial Differential Complementarity Problems
Authors: Hout Karel J. in’t , Snoeijer Jacob
Published: June 2021

We study the principal component analysis based approach introduced by Reisinger and Wittum (2007) and the comonotonic approach considered by Hanbali and Linders (2019) for the approximation of American basket option values via multidimensional partial differential complementarity problems (PDCPs). Both approximation approaches require the solution of just a limited number of low-dimensional PDCPs. It is demonstrated by ample numerical experiments that they define approximations that lie close to each other. Next, an efficient discretisation of the pertinent PDCPs is presented that leads to a favourable convergence behaviour.
On Hyers–Ulam and Hyers–Ulam–Rassias Stability of a Nonlinear Second-Order Dynamic Equation on Time Scales
Authors: Hamza Alaa E. , Alghamdi Maryam A. , Alharbi Mymonah S.
Published: June 2021

In this paper, we obtain sufficient conditions for Hyers–Ulam and Hyers–Ulam–Rassias stability of an abstract second–order nonlinear dynamic equation on bounded time scales. An illustrative example is given to show the applicability of the theoretical results.
Marine Alkaloids: Compounds with In Vivo Activity and Chemical Synthesis
Authors: Munekata Paulo E. S. , Pateiro Mirian , Conte-Junior Carlos A. , Domínguez Rubén , Nawaz Asad , Walayat Noman , Movilla Fierro Elena , Lorenzo José M.
Published: June 2021

Marine alkaloids comprise a class of compounds with several nitrogenated structures that can be explored as potential natural bioactive compounds. The scientific interest in these compounds has been increasing in the last decades, and many studies have been published elucidating their chemical structure and biological effects in vitro. Following this trend, the number of in vivo studies reporting the health-related properties of marine alkaloids has been increasing and providing more information about the effects in complex organisms. Experiments with animals, especially mice and zebrafish, are revealing the potential health benefits against cancer development, cardiovascular diseases, seizures, Alzheimer’s disease, mental health disorders, inflammatory diseases, osteoporosis, cystic fibrosis, oxidative stress, human parasites, and microbial infections in vivo. Although major efforts are still necessary to increase the knowledge, especially about the translation value of the information obtained from in vivo experiments to clinical trials, marine alkaloids are promising candidates for further experiments in drug development.
The Perspective and Challenge of Nanomaterials in Oil and Gas Wastewater Treatment
Authors: Liu Xiaoying , Ruan Wenlin , Wang Wei , Zhang Xianming , Liu Yunqi , Liu Jingcheng
Published: June 2021

Oil and gas wastewater refers to the waste stream produced in special production activities such as drilling and fracturing. This kind of wastewater has the following characteristics: high salinity, high chromaticity, toxic and harmful substances, poor biodegradability, and a difficulty to treat. Interestingly, nanomaterials show great potential in water treatment technology because of their small size, large surface area, and high surface energy. When nanotechnology is combined with membrane treatment materials, nanofiber membranes with a controllable pore size and high porosity can be prepared, which provides more possibilities for oil–water separation. In this review, the important applications of nanomaterials in wastewater treatment, including membrane separation technology and photocatalysis technology, are summarized. Membrane separation technology is mainly manifested in ultrafiltration (UF), nanofiltration (NF), and reverse osmosis (RO). It also focuses on the application of semiconductor photocatalysis technology induced by TiO2 in the degradation of oil and gas wastewater. Finally, the development trends of nanomaterials in oil and gas wastewater treatment are prospected.
Dynamics of Monolayer Growth in Vapor–Liquid–Solid GaAs Nanowires Based on Surface Energy Minimization
Authors: Hijazi Hadi , Dubrovskii Vladimir
Published: June 2021

The vapor–liquid–solid growth of III-V nanowires proceeds via the mononuclear regime, where only one island nucleates in each nanowire monolayer. The expansion of the monolayer is governed by the surface energetics depending on the monolayer size. Here, we study theoretically the role of surface energy in determining the monolayer morphology at a given coverage. The optimal monolayer configuration is obtained by minimizing the surface energy at different coverages for a set of energetic constants relevant for GaAs nanowires. In contrast to what has been assumed so far in the growth modeling of III-V nanowires, we find that the monolayer expansion may not be a continuous process. Rather, some portions of the already formed monolayer may dissolve on one of its sides, with simultaneous growth proceeding on the other side. These results are important for fundamental understanding of vapor–liquid–solid growth at the atomic level and have potential impacts on the statistics within the nanowire ensembles, crystal phase, and doping properties of III-V nanowires.
The Interplay between Nutrition, Innate Immunity, and the Commensal Microbiota in Adaptive Intestinal Morphogenesis
Authors: Bayer Franziska , Dremova Olga , Khuu My Phung , Mammadova Könül , Pontarollo Giulia , Kiouptsi Klytaimnistra , Soshnikova Natalia , May-Simera Helen Louise , Endres Kristina , Reinhardt Christoph
Published: June 2021

The gastrointestinal tract is a functionally and anatomically segmented organ that is colonized by microbial communities from birth. While the genetics of mouse gut development is increasingly understood, how nutritional factors and the commensal gut microbiota act in concert to shape tissue organization and morphology of this rapidly renewing organ remains enigmatic. Here, we provide an overview of embryonic mouse gut development, with a focus on the intestinal vasculature and the enteric nervous system. We review how nutrition and the gut microbiota affect the adaptation of cellular and morphologic properties of the intestine, and how these processes are interconnected with innate immunity. Furthermore, we discuss how nutritional and microbial factors impact the renewal and differentiation of the epithelial lineage, influence the adaptation of capillary networks organized in villus structures, and shape the enteric nervous system and the intestinal smooth muscle layers. Intriguingly, the anatomy of the gut shows remarkable flexibility to nutritional and microbial challenges in the adult organism.
Association between Hyperglycemia at Hospital Presentation and Hospital Outcomes in COVID-19 Patients with and without Type 2 Diabetes: A Retrospective Cohort Study of Hospitalized Inner-City COVID-19 Patients
Authors: Charoenngam Nipith , Alexanian Sara M. , Apovian Caroline M. , Holick Michael F.
Published: June 2021

This study aimed to determine the relationships among hyperglycemia (HG), the presence of type 2 diabetes (T2D), and the outcomes of COVID-19. Demographic data, blood glucose levels (BG) measured on admission, and hospital outcomes of COVID-19 patients hospitalized at Boston University Medical Center from 1 March to 4 August 2020 were extracted from the hospital database. HG was defined as BG > 200 mg/dL. Patients with type 1 diabetes or BG < 70 mg/dL were excluded. A total of 458 patients with T2D and 976 patients without T2D were included in the study. The mean ± SD age was 56 ± 17 years and 642 (45%) were female. HG occurred in 193 (42%) and 42 (4%) of patients with and without T2D, respectively. Overall, the in-hospital mortality rate was 9%. Among patients without T2D, HG was statistically significantly associated with mortality, ICU admission, intubation, acute kidney injury, and severe sepsis/septic shock, after adjusting for potential confounders (p < 0.05). However, only ICU admission and acute kidney injury were associated with HG among patients with T2D (p < 0.05). Among the 235 patients with HG, the presence of T2D was associated with decreased odds of mortality, ICU admission, intubation, and severe sepsis/septic shock, after adjusting for potential confounders, including BG (p < 0.05). In conclusion, HG in the subset of patients without T2D could be a strong indicator of high inflammatory burden, leading to a higher risk of severe COVID-19.
Community-Based Efforts Aim to Improve the Food Environment within a Highly Obese Rural Appalachian County
Authors: Gillespie Rachel , DeWitt Emily , Norman-Burgdolf Heather , Dunnaway Brynnan , Gustafson Alison
Published: June 2021

Rural communities in Appalachia are displaying increased obesity prevalence, yet traditional interventions have not provided a broad enough impact to improve dietary consumption patterns. Therefore, expanding efforts that address the food environment and incorporate behavioral nudges through community-developed marketing strategies may be a viable mechanism to improve food and beverage choices within this unique population. This study installed shelf-wobblers across n = 5 gas stations in one rural Appalachian county in Kentucky. Smart Snacks were identified from store inventory lists utilizing the CDC Food Service Guideline for Federal Facilities calculator and were categorized into high-protein snacks, low-fat carbohydrate snacks, meal replacement snacks, and no-calorie beverages. NEMS-CS audits were conducted, and monthly sales data was collected at baseline and for six months thereafter for each store location. A difference-in-difference model was used, adjusting for total sales or total mean sales for each Smart Snack model to assess the percentage change within and between stores. Overall, percent change in mean sales and total sales across all stores resulted in a percentage increase of sales of Smart Snack items following wobbler installment. This study provides unique insight into how a community-driven approach to marketing can influence the sale of healthier food and beverage items.
Inflammatory Potential of the Diet and Incidence of Crohn’s Disease and Ulcerative Colitis in the EPIC-Spain Cohort
Authors: Guevara Marcela , Salamanca-Fernández Elena , Miqueleiz Estrella , Gavrila Diana , Amiano Pilar , Bonet Catalina , Rodríguez-Barranco Miguel , Huerta José María , Bujanda Luis , Sánchez María José , Chirlaque María Dolores , Agudo Antonio , Ardanaz Eva , Castilla Jesús
Published: June 2021

Diet may influence the development of inflammatory bowel disease through the modulation of inflammation. We investigated whether the inflammatory potential of the diet is associated with the risk of Crohn’s disease (CD) and ulcerative colitis (UC) in the Spanish cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC-Spain). The study included 32,633 participants aged 29–69 years. The inflammatory potential of the diet was measured by using an inflammatory score of the diet (ISD) based on a baseline dietary history questionnaire. Cox regression was used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). During 21 years (674,547 person-years) of follow-up, 32 and 57 participants developed CD and UC, respectively. In multivariable analysis, a one-standard deviation (SD) increment in the ISD (two-unit increase) was associated with a higher risk of CD (HR of 1.71; 95% CI: 1.05–2.80; p = 0.031). By contrast, ISD was not associated with UC (HR for one-SD increment of 0.89; 95% CI: 0.66–1.19; p = 0.436). Our results suggest that consuming a more pro-inflammatory diet may contribute to the risk of CD, supporting that a healthy diet might be beneficial in its prevention. Further, larger studies are needed to verify these findings.
Special Issue “Maternal DHA Impact on Child Neurodevelopment”
Authors: Duttaroy Asim
Published: June 2021

In this special issue, we have focused on the maternal docosahexaenoic acid, 22:6n-3 (DHA), on children’s neurodevelopment [...]
Coffee Consumption and Cardiovascular Diseases: A Mendelian Randomization Study
Authors: Yuan Shuai , Carter Paul , Mason Amy M. , Burgess Stephen , Larsson Susanna C.
Published: June 2021

Coffee consumption has been linked to a lower risk of cardiovascular disease in observational studies, but whether the associations are causal is not known. We conducted a Mendelian randomization investigation to assess the potential causal role of coffee consumption in cardiovascular disease. Twelve independent genetic variants were used to proxy coffee consumption. Summary-level data for the relations between the 12 genetic variants and cardiovascular diseases were taken from the UK Biobank with up to 35,979 cases and the FinnGen consortium with up to 17,325 cases. Genetic predisposition to higher coffee consumption was not associated with any of the 15 studied cardiovascular outcomes in univariable MR analysis. The odds ratio per 50% increase in genetically predicted coffee consumption ranged from 0.97 (95% confidence interval (CI), 0.63, 1.50) for intracerebral hemorrhage to 1.26 (95% CI, 1.00, 1.58) for deep vein thrombosis in the UK Biobank and from 0.86 (95% CI, 0.50, 1.49) for subarachnoid hemorrhage to 1.34 (95% CI, 0.81, 2.22) for intracerebral hemorrhage in FinnGen. The null findings remained in multivariable Mendelian randomization analyses adjusted for genetically predicted body mass index and smoking initiation, except for a suggestive positive association for intracerebral hemorrhage (odds ratio 1.91; 95% CI, 1.03, 3.54) in FinnGen. This Mendelian randomization study showed limited evidence that coffee consumption affects the risk of developing cardiovascular disease, suggesting that previous observational studies may have been confounded.
Water Intake in Pregnant Women in China, 2018: The Report of a Survey
Authors: Ding Ye , Xie Zhencheng , Lu Xiaolong , Luo Hongliang , Pan Han , Lin Xiaofang , Wu Jieshu , Wang Zhixu
Published: June 2021

Background: Adequate water intake in pregnant women plays an important role in their health and in fetal growth and development. However, there is insufficient applicable data to guide and evaluate the water intake of pregnant women in China. Based on a nationwide sample of pregnant women, we mainly aimed to investigate the daily total water intake (TWI) and the contribution of different beverages and food sources to the TWI, to assess the percentage of participants who comply with the adequate intake (AI) value of water set by the Chinese Nutrition Society (CNS) and the European Food Safety Authority (EFSA) and to analyze the contribution of different water sources to the daily total energy intake (TEI). Methods: A multi-stage sampling method was used to recruit pregnant women from 11 provinces and two municipalities in China. A 4-day online diary with a food atlas was used to assess water and dietary intake. Finally, 653 pregnant women were included in the analysis. The Mann–Whitney U test and the independent-sample t-test were used to compare the differences between related variables in different age groups or different gestational periods, and partial correlation was used to explore the correlation between water and energy intake. Results: The median daily TWI of pregnant women was 2190 mL, of which water from beverages and foods accounted for 52.9% and 47.1%, respectively. Approximately 80.5% of the water from beverages was mainly from plain water (r = 0.973), while in the part of the water from foods, dishes (32.4%) were the main contributors (r = 0.663). Only 16.4% and 43.8% of the total population met the TWI recommendation set by the CNS and EFSA, respectively. Among these, the contribution of the water from beverages was higher than that of the water from food. For those whose TWI did not reach the recommended level, the contribution of the water from beverages was almost equal to that of food. The median daily TEI of pregnant women was 1589 kcal, of which beverages accounted for 9.7%. Milk and milk derivatives (71.3%) were the main contributors to energy from beverages, accounting for 71.3% (r = 0.444). Although sugar-sweetened drinks only accounted for 10.1% of the energy from beverages, they were highly correlated with energy from beverages (r = 0.836). Through grouping analysis, age and gestational period had no significant effect on the above main results. Conclusions: This was the beginning of a nationwide study on the TWI of pregnant women in China, and the results provide evidence of the need for interventions to improve water intake among pregnant women and the revision of reference values for AI of TWI in pregnant women in China.
Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial
Authors: Khotib Junaidi , Gani Maria Apriliani , Budiatin Aniek Setiya , Lestari Maria Lucia Ardhani Dwi , Rahadiansyah Erreza , Ardianto Chrismawan
Published: June 2021

Bone defects and periodontal disease are pathological conditions that may become neglected diseases if not treated properly. Hydroxyapatite (HA), along with tricalcium phosphate and bioglass ceramic, is a biomaterial widely applied to orthopedic and dental uses. The in vivo performance of HA is determined by the interaction between HA particles with bone cells, particularly the bone mineralizing cells osteoblasts. It has been reported that HA-induced osteoblastic differentiation by increasing the expression of osteogenic transcription factors. However, the pathway involved and the events that occur in the cell membrane have not been well understood and remain controversial. Advances in gene editing and the discovery of pharmacologic inhibitors assist researchers to better understand osteoblastic differentiation. This review summarizes the involvement of extracellular signal-regulated kinase (ERK), p38, Wnt, and bone morphogenetic protein 2 (BMP2) in osteoblastic cellular regulation induced by HA. These advances enhance the current understanding of the molecular mechanism of HA as a biomaterial. Moreover, they provide a better strategy for the design of HA to be utilized in bone engineering.
Serum Extracellular Vesicle-Derived circHIPK3 and circSMARCA5 Are Two Novel Diagnostic Biomarkers for Glioblastoma Multiforme
Authors: Stella Michele , Falzone Luca , Caponnetto Angela , Gattuso Giuseppe , Barbagallo Cristina , Battaglia Rosalia , Mirabella Federica , Broggi Giuseppe , Altieri Roberto , Certo Francesco , Caltabiano Rosario , Barbagallo Giuseppe Maria Vincenzo , Musumeci Paolo , Ragusa Marco , Pietro Cinzia Di , Libra Massimo , Purrello Michele , Barbagallo Davide
Published: June 2021

Glioblastoma multiforme (GBM) is the most frequent and deadly human brain cancer. Early diagnosis through non-invasive biomarkers may render GBM more easily treatable, improving the prognosis of this currently incurable disease. We suggest the use of serum extracellular vesicle (sEV)-derived circular RNAs (circRNAs) as highly stable minimally invasive diagnostic biomarkers for GBM diagnosis. EVs were isolated by size exclusion chromatography from sera of 23 GBM and 5 grade 3 glioma (GIII) patients, and 10 unaffected controls (UC). The expression of two candidate circRNAs (circSMARCA5 and circHIPK3) was assayed by droplet digital PCR. CircSMARCA5 and circHIPK3 were significantly less abundant in sEVs from GBM patients with respect to UC (fold-change (FC) of −2.15 and −1.92, respectively) and GIII (FC of −1.75 and −1.4, respectively). Receiver operating characteristic curve (ROC) analysis, based on the expression of sEV-derived circSMARCA5 and circHIPK3, allowed us to distinguish GBM from UC (area under the curve (AUC) 0.823 (0.667–0.979) and 0.855 (0.704 to 1.000), with a 95% confidence interval (CI), respectively). Multivariable ROC analysis, performed by combining the expression of sEV-derived circSMARCA5 and circHIPK3 with preoperative neutrophil to lymphocyte (NLR), platelet to lymphocyte (PLR) and lymphocyte to monocyte (LMR) ratios, three known diagnostic and prognostic GBM markers, allowed an improvement in the GBM diagnostic accuracy (AUC 0.901 (0.7912 to 1.000), 95% CI). Our data suggest sEV-derived circSMARCA5 and circHIPK3 as good diagnostic biomarkers for GBM, especially when associated with preoperative NLR, PLR and LMR.
Aptamer Applications in Emerging Viral Diseases
Authors: Krüger Arne , de Jesus Santos Ana Paula , de Sá Vanessa , Ulrich Henning , Wrenger Carsten
Published: June 2021

Aptamers are single-stranded DNA or RNA molecules which are submitted to a process denominated SELEX. SELEX uses reiterative screening of a random oligonucleotide library to identify high-affinity binders to a chosen target, which may be a peptide, protein, or entire cells or viral particles. Aptamers can rival antibodies in target recognition, and benefit from their non-proteic nature, ease of modification, increased stability, and pharmacokinetic properties. This turns them into ideal candidates for diagnostic as well as therapeutic applications. Here, we review the recent accomplishments in the development of aptamers targeting emerging viral diseases, with emphasis on recent findings of aptamers binding to coronaviruses. We focus on aptamer development for diagnosis, including biosensors, in addition to aptamer modifications for stabilization in body fluids and tissue penetration. Such aptamers are aimed at in vivo diagnosis and treatment, such as quantification of viral load and blocking host cell invasion, virus assembly, or replication, respectively. Although there are currently no in vivo applications of aptamers in combating viral diseases, such strategies are promising for therapy development in the future.
Drug Delivery via the Suprachoroidal Space for the Treatment of Retinal Diseases
Authors: Naftali Ben Haim Liron , Moisseiev Elad
Published: June 2021

The suprachoroidal space (SCS), a potential space between the sclera and choroid, is becoming an applicable method to deliver therapeutics to the back of the eye. In recent years, a vast amount of research in the field has been carried out, with new discoveries in different areas of interest, such as imaging, drug delivery methods, pharmacokinetics, pharmacotherapies in preclinical and clinical trials and advanced therapies. The SCS can be visualized via advanced techniques of optical coherence tomography (OCT) in eyes with different pathologies, and even in healthy eyes. Drugs can be delivered easily and safely via hollow microneedles fitted to the length of the approximate thickness of the sclera. SCS injections were found to reach greater baseline concentrations in the target layers compared to intravitreal (IVT) injection, while agent clearance was faster with highly aqueous soluble molecules. Clinical trials with SCS injection of triamcinolone acetonide (TA) were executed with promising findings for patients with noninfectious uveitis (NIU), NIU implicated with macular edema and diabetic macular edema (DME). Gene therapy is evolving rapidly with viral and non-viral vectors that were found to be safe and efficient in preclinical trials. Here, we review these novel different aspects and new developments in clinical treatment of the posterior segment of the eye.
Radioimmunotheranostic Pair Based on the Anti-HER2 Monoclonal Antibody: Influence of Chelating Agents and Radionuclides on Biological Properties
Authors: Miranda Ana Cláudia Camargo , dos Santos Sofia Nascimento , Fuscaldi Leonardo Lima , Balieiro Luiza Mascarenhas , Bellini Maria Helena , Guimarães Maria Inês Calil Cury , de Araújo Elaine Bortoleti
Published: June 2021

The oncogene HER2 is an important molecular target in oncology because it is associated with aggressive disease and the worst prognosis. The development of non-invasive imaging techniques and target therapies using monoclonal antibodies is a rapidly developing field. Thus, this work proposes the study of the radioimmunotheranostic pair, [111In]In-DTPA-trastuzumab and [177Lu]Lu-DOTA-trastuzumab, evaluating the influence of the chelating agents and radionuclides on the biological properties of the radioimmunoconjugates (RICs). The trastuzumab was immunoconjugated with the chelators DTPA and DOTA and radiolabeled with [111In]InCl3 and [177Lu]LuCl3, respectively. The stability of the RICs was evaluated in serum, and the immunoreactive and internalization fractions were determined in SK-BR-3 breast cancer cells. The in vivo pharmacokinetics and dosimetry quantification and the ex vivo biodistribution were performed in normal and SK-BR-3 tumor-bearing mice. The data showed that there was no influence of the chelating agents and radionuclides on the immunoreactive and internalization fractions of RICs. In contrast, they influenced the stability of RICs in serum, as well as the pharmacokinetics, dosimetry and biodistribution profiles. Therefore, the results showed that the nature of the chelating agent and radionuclide could influence the biological properties of the radioimmunotheranostic pair.
Cholesterol Levels Affect the Performance of AuNPs-Decorated Thermo-Sensitive Liposomes as Nanocarriers for Controlled Doxorubicin Delivery
Authors: García Mónica C. , Naitlho Nabila , Calderón-Montaño José Manuel , Drago Estrella , Rueda Manuela , Longhi Marcela , Rabasco Antonio M. , López-Lázaro Miguel , Prieto-Dapena Francisco , González-Rodríguez María Luisa
Published: June 2021

Stimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D) delivery. Their interfacial and morphological properties, drug release behavior against temperature changes and cytotoxic activity against breast and ovarian cancer cells were studied. Langmuir isotherms were performed to identify the most stable combination of lipid components. Two mole fractions of cholesterol (3.35 mol% and 40 mol%, L1 and L2 series, respectively) were evaluated. Thin-film hydration and transmembrane pH-gradient methods were used for preparing the L and for D loading, respectively. The cationic surface of L allowed the anchoring of negatively charged AuNPs by electrostatic interactions, even inducing a shift in the zeta potential of the L2 series. L exhibited nanometric sizes and spherical shape. The higher the proportion of cholesterol, the higher the drug loading. D was released in a controlled manner by diffusion-controlled mechanisms, and the proportions of cholesterol and temperature of release media influenced its release profiles. D-encapsulated L preserved its antiproliferative activity against cancer cells. The developed liposomal formulations exhibit promising properties for cancer treatment and potential for hyperthermia therapy.
Dual-Acting Zeta-Potential-Changing Micelles for Optimal Mucus Diffusion and Enhanced Cellular Uptake after Oral Delivery
Authors: Malkawi Ahmad , Alrabadi Nasr , Kennedy Ross Allan
Published: June 2021

Context: Overcoming the intestinal mucosal barrier can be a challenge in drug delivery. Nanoemulsions with negative zeta potentials can effectively permeate the mucus layer, but those with positive zeta potentials are better taken up by cells; a nanoemulsion with capricious zeta potential from negative to positive can achieve both good permeation and high uptake. Objective: This study aimed to develop dual-acting zeta-potential-amphoteric micelles enabling optimal muco-permeation and enhancement of cellular uptake. Methods: A micellar pre-concentrate was prepared from 15% Labrasol, 15% Kolliphor EL, 30% Kolliphor RH 40, and 40% dimethylsulfoxide. The micellar pre-concentrate was loaded with anionic stearic acid (SA), forming ionic complexes with cationic polymers at a ratio of 25:1 with Eudragit RS 100 and Eudragit RL 100. Blank micelles and those containing complexes were separately diluted in physiological buffers and examined for their droplet sizes, polydispersity indices (PDIs), zeta potentials, and cytotoxicity. The SA release from the micellar complexes was evaluated in 0.1 mM phosphate buffer (pH 6.8) containing 0.001% fluorescein, thereby enabling an instant decrease in fluorescence. Finally, the micelles were loaded with the model drug fluorescein diacetate (FDA) and evaluated for their muco-permeation behavior and cellular uptake. Results: The micellar dilutions formed micelles at the critical micelle concentration (CMC) of 312 µg/mL and showed a uniform average droplet size of 14.2 nm, with a PDI < 0.1. Micellar dilutions were non-cytotoxic when used at 1:100 in a physiological medium. Micelles loaded with ionic complexes achieved a sustained release of 95.5 ± 3.7% of the SA in 180 min. Moreover, the zeta potential of the complex-loaded micelles shifted from −5.4 to +1.8 mV, whereas the blank micelles showed a stabilized zeta potential of −10 mV. Furthermore, the negatively charged blank and complex-loaded micelles exhibited comparable muco-permeation, with an overall average of 58.2 ± 3.7% diffusion of FDA. The complex-loaded micellar droplets, however, provided a significantly higher cellular uptake of the model drug FDA (2.2-fold, p ≤ 0.01) Conclusion: Due to undergoing a shift in zeta potential, the modified micelles significantly enhanced cellular uptake while preserving mucus-permeating properties.
SIR-PID: A Proportional–Integral–Derivative Controller for COVID-19 Outbreak Containment
Authors: Ianni Aldo , Rossi Nicola
Published: June 2021

Ongoing social restrictions, including social distancing and lockdown, adopted by many countries to inhibit spread of the the COVID-19 epidemic, must attempt to find a trade-off between induced economic damage, healthcare system collapse, and the costs in terms of human lives. Applying and removing restrictions on a system with a given latency as represented by an epidemic outbreak (and formally comparable with mechanical inertia), may create critical instabilities, overshoots, and strong oscillations in the number of infected people around the desirable set-point, defined in a practical way as the maximum number of hospitalizations acceptable by a given healthcare system. A good understanding of the system reaction to any change of the input control variable can be reasonably achieved using a proportional–integral–derivative controller (PID), which is a widely used technique in various physics and technological applications. In this paper, this control theory to is proposed to be applied epidemiology, to understand the reaction of COVID-19 propagation to social restrictions and to reduce epidemic damages through the correct tuning of the containment policy. Regarding the synthesis of this interdisciplinary approach, the extended to the susceptible–infectious–recovered (SIR) model name “SIR-PID” is suggested.
Chelating Agents Usage in Optimization of Fracturing Fluid Rheology Prepared from Seawater
Authors: Othman Amro , Aljawad Murtada Saleh , Mahmoud Mohamed , Kamal Muhammad Shahzad , Patil Shirish , Bataweel Mohammed
Published: June 2021

Hydraulic fracturing consumes massive volumes of freshwater that is usually scarce and costly. Such operation is not sustainable, and hence seawater could be used as an alternative. Nevertheless, seawater has high total dissolved solids (TDS), affecting the fracturing fluid rheology and providing a damage potential to the subterranean hydrocarbon reservoirs. Resolving these issues requires fracturing fluid systems with many additives, which results in an expensive and non-eco-friendly system. This study proposes eco-friendly and biodegradable chelating agents that could replace many additives such as scale inhibitors and crosslinkers. The study aims to optimize the rheology of seawater fracturing fluids using a chelating agent and polymer. By optimizing N,N-Dicarboxymethyl glutamic acid (GLDA) conditions, high viscosity was achieved using a standard industry rheometer. The GLDA was mixed with carboxymethyl hydroxypropyl guar (CMHPG) polymer and tested in both deionized water (DW) and seawater (SW). The polymer was examined first, where the rheology did not show a time-dependent behavior. The polymer in SW showed a slightly higher viscosity than in DW. The GLDA and CMHPG were tested at different temperatures, pH, and concentrations. These sets showed a time-dependent viscosity behavior, which can be utilized in various fracturing steps. Results showed that the solution pH and GLDA concentration significantly impacted the fluid viscosity magnitude and behavior. The developed formulation is shear thinning, where the viscosity declines as the shear rate increases. The temperature negatively impacted the viscosity and caused the formulation to break. The study provided an understanding of how to optimize the rheology of SW fracturing fluid based on GLDA chelating and CMHPG polymer.
Ageing Effects in Mounting Media of Microscope Slide Samples from Natural History Collections: A Case Study with Canada Balsam and PermountTM
Authors: Schmid Thomas , Hidde Julia , Grünier Sophie , Jungnickel Robert , Dariz Petra , Riedel Jens , Neuhaus Birger
Published: June 2021

Microscope slide collections represent extremely valuable depositories of research material in a natural history, forensic, veterinary, and medical context. Unfortunately, most mounting media of these slides deteriorate over time, with the reason for this not yet understood at all. In this study, Raman spectroscopy, ultraviolet–visible (UV–Vis) spectroscopy, and different types of light microscopy were used to investigate the ageing behaviour of naturally aged slides from museum collections and the experimentally aged media of Canada balsam and Permount™, representing a natural and a synthetic resin, respectively, with both being based on mixtures of various terpenes. Whereas Canada balsam clearly revealed chemical ageing processes, visible as increasing colouration, Permount™ showed physical deterioration recognisable by the increasing number of cracks, which even often impacted a mounted specimen. Noticeable changes to the chemical and physical properties of these mounting media take decades in the case of Canada balsam but just a few years in the case of Permount™. Our results question whether or not Canada balsam should really be regarded as a mounting medium that lasts for centuries, if its increasing degree of polymerisation can lead to a mount which is no longer restorable.
Graphene-Assisted Polymer Waveguide Optically Controlled Switch Using First-Order Mode
Authors: Yang Yue , Lv Jiawen , Lin Baizhu , Cao Yue , Yi Yunji , Zhang Daming
Published: June 2021

All-optical devices have a great potential in optical communication systems. As a new material, graphene has attracted great attention in the field of optics due to its unique properties. We propose a graphene-assisted polymer optically controlled thermo-optic switch, based on the Ex01 mode, which can reduce the absorption loss of graphene. Graphene absorbs 980 nm pump light, and uses the heat generated by ohmic heating to switch on and off the signal light at 1550 nm. The simulation results show that, when the graphene is in the right position, we can obtain the power consumption of 9.5 mW, the propagation loss of 0.01 dB/cm, and the switching time of 127 μs (rise)/125 μs (fall). The switching time can be improved to 106 μs (rise) and 102 μs (fall) with silicon substrate. Compared with an all-fiber switch, our model has lower power consumption and lower propagation loss. The proposed switch is suitable for optically controlled fields with low loss and full polarization. Due to the low cost and easy integration of polymer materials, the device will play an important role in the fields of all-optical signal processing and silicon-based hybrid integrated photonic devices.
“Only I Am the Brahma”: Religion and Narrative in the Netflix Thriller Series Sacred Games (2018–2019)
Authors: Ashraf Ana
Published: June 2021

Sacred Games (2018–2019), based on Vikram Chandra’s novel of the same title, is India’s first Netflix crime thriller series. This series shows how the lives of a Sikh policeman, Sartaj Singh, and a powerful gangster, Ganesh Eknath Gaitonde, weave together in a mission to save Mumbai from a nuclear attack. The series immediately received critical acclaim and viewers’ appreciation, but the way the series represents the (mis)use of metanarratives of religious and political ideologies, as they come to influence Gaitonde’s life, needs further perusal. For this purpose, this article investigates how Gaitonde’s life, and its abrupt end, are shaped and challenged by the larger ideological and religious metanarratives of his milieu. At the same time, this article examines Gaitonde’s ability to gain control over his own narrative despite the overwhelming presence of these metanarratives. More specifically, Gaitonde’s transgressive will and his desire to tell his story are brought under scrutiny. Along with the analysis of Gaitonde’s character, this article also examines how the use of various cinematic and narrative techniques heightens self-reflexivity and metafictionality in Sacred Games and emphasizes the role of mini-narratives as unique, singular, and contingent, in contrast to the generic, universal, and permanent tones of metanarratives.
A Compressed and High-Accuracy Star Tracker with On-Orbit Deployable Baffle for Remote Sensing CubeSats
Authors: Liu Xinyuan , Xing Fei , Fan Shaoyan , You Zheng
Published: June 2021

CubeSats have been widely used in remote sensing applications such as global coverage, hotspots revisited, etc. However, due to the strict size limitation, the high-accuracy measuring instruments such as star tracker are too large to be applied in CubeSat, thus causing insufficient accuracy in satellite attitude and image positioning. In order to reduce the volume of star tracker without compromising the performance, the relationship between the volume and pointing accuracy or dynamic performance is studied and an optimization model of star tracker with a minimum volume is proposed. Compared with the traditional star tracker, a deployable star tracker with a novel deployable baffle and surrounded circuit structure is designed. The baffle consists of nested three-stage sub-baffles with a scientifically analyzed and verified taper to achieve smooth deployment and compression. The special circuit structure surrounds the lens and can be compressed in the inner sub-baffle. Therefore, the deployable star tracker can be compressed to the smallest volume and the sub-baffles can be deployed to the accurate position without self-lock risk. The experimental results verify its deployment accuracy and reliability as well as space environmental adaptability. The deployable star tracker has almost the same results on stray light suppression ability, pointing accuracy (better than 3″ (3σ)) and dynamic performance (up to 3°/s) with the traditional star tracker. Furthermore, an integrated attitude determination and control system based on the deployable star tracker for CubeSat is further designed and implemented to support high-accuracy remote sensing.
Integrated Laser Scanner Techniques to Produce High-Resolution DTM of Vegetated Territory
Authors: Marotta Federica , Teruggi Simone , Achille Cristiana , Vassena Giorgio Paolo Maria , Fassi Francesco
Published: June 2021

The paper presents the first part of a research project concerning the creation of 3D terrain models useful to understand landslide movements. Thus, it illustrates the creation process of a multi-source high-resolution Digital Terrain Model (DTM) in very dense vegetated areas obtained by integrating 3D data coming from three sources, starting from long and medium-range Terrestrial Laser Scanner up to a Backpack Indoor Mobile Mapping System. The point clouds are georeferenced by means of RKT GNSS points and automatically filtered using a Cloth Simulation Filter algorithm to separate points belonging to the ground. Those points are interpolated to produce the DTMs which are then mosaicked to obtain a unique multi-resolution DTM that plays a crucial role in the detection and identification of specific geological features otherwise visible. Standard deviation of residuals of the DTM varies from 0.105 m to 0.176 m for Z coordinate, from 0.065 m to 0.300 m for X and from 0.034 m to 0.175 m for Y. The area under investigation belongs to the Municipality of Piuro (SO) and includes both the town and surrounding valley. It was affected by a dramatic landslide in 1618 that destroyed the entire village. Numerous challenges have been faced, caused both by the characteristics of the area and the processed data. The complexity of the case study turns out to be an excellent test bench for the employed technologies, providing the opportunity to precisely identify the needed direction to obtain future promising results.
Construction and Application of a Knowledge Graph
Authors: Hao Xuejie , Ji Zheng , Li Xiuhong , Yin Lizeyan , Liu Lu , Sun Meiying , Liu Qiang , Yang Rongjin
Published: June 2021

With the development and improvement of modern surveying and remote-sensing technology, data in the fields of surveying and remote sensing have grown rapidly. Due to the characteristics of large-scale, heterogeneous and diverse surveys and the loose organization of surveying and remote-sensing data, effectively obtaining information and knowledge from data can be difficult. Therefore, this paper proposes a method of using ontology for heterogeneous data integration. Based on the heterogeneous, decentralized, and dynamic updates of large surveying and remote-sensing data, this paper constructs a knowledge graph for surveying and remote-sensing applications. First, data are extracted. Second, using the ontology editing tool Protégé, a knowledge graph mode level is constructed. Then, using a relational database, data are stored, and a D2RQ tool maps the data from the mode level’s ontology to the data layer. Then, using the D2RQ tool, a SPARQL protocol and resource description framework query language (SPARQL) endpoint service is used to describe functions such as query and reasoning of the knowledge graph. The graph database is then used to display the knowledge graph. Finally, the knowledge graph is used to describe the correlation between the fields of surveying and remote sensing.