bluuebunny/crossref_metadata_2025 huggingface dataset:
Arsenic-Induced Mitochondria-Mediated Apoptosis is Associated with PGC-1α Decrease in Rat Brain
Authors: Ding Bo , Ma Xinbo , Liu Yang , Ni Bangyao , Lu Siqi , Chen Yuting , Liu Xiaona , Zhang Wei
Published: May 2023

Chronic exposure to arsenic in drinking-water damage to cognitive function, and nerve cells apoptosis is one of primary characteristic. The damage of mitochondrial structure and/or function is one of the main characteristics of apoptosis. Peroxisome proliferator activated receptor γ Coactivator α (PGC-1α) is involved in the regulation of mitochondrial biogenesis, energy metabolism and apoptosis. In this study, we aimed to study role of PGC-1α in sodium arsenite (NaAsO2)-induced mitochondrial apoptosis in rat hippocampal cells. We discovered that arsenic-induced apoptosis increased in rat hippocampus increased with NaAsO2 (0, 2, 10, and 50 mg/L, orally via drinking water for 12 weeks) exposure by TUNEL assay, and the structure of mitochondria was incomplete, swollen, lysosomes and lipofuscin increasing, and nuclear membrane shrunk observed by transmission electron microscope. Furthermore, NaAsO2 reduced levels of Bcl-2 and PGC-1α, increased the levels of Bax and Cytochrome C expression. Moreover, correlation analysis showed that brain arsenic content was negatively correlated with PGC-1α level and brain ATP content, respectively; PGC-1α level was negatively correlated with apoptosis rate; Brain ATP content was positively correlated with PGC-1α level; but no significant correlation between ATP content and apoptosis has been observed in this study. Taken together, the results of present study indicate that NaAsO2-induced mitochondrial pathway apoptosis is related to the reduction of PGC-1α, accompanied by ATP depletion.
Improved Arterial Oxygenation by High Flow Nasal Cannula Oxygen Therapy in Older Patients with Severe Respiratory Failure in a Non-intensive Hospital Ward
Authors: Fimognari Filippo Luca , Bambara Valentina , Armentaro Giuseppe , Scarpino Paola , Settino Chiara , Filice Marco , Rizzo Massimo , Sciacqua Angela
Published: May 2023

Background. There are scant data about the effectiveness of high flow nasal cannula (HFNC) oxygen therapy in patients hospitalized with severe acute respiratory failure (ARF) in non-intensive medical wards, particularly regarding the effect on arterial oxygenation compared to conventional oxygen therapy (COT) and non-invasive ventilation (NIV) or continuous positive airway pressure (CPAP).
Methods. In a retrospective observational study, oxygenation parameters were measured before and immediately after HFNC initiation in 37 consecutive patients hospitalized in a geriatric ward in 2017.
Results. HFNC was used as escalation therapy for untreatable hypoxia after failure of NIV/CPAP (n=18) or COT (n=19). Twenty-two patients died, 2 were transferred to the intensive care unit, while 13 were discharged alive. A “do not intubate” status was identified in 17 of the 22 deceased patients. Partial pressure of oxygen (pO2, p< 0.0001), oxygen saturation (SO2, p< 0.0001), pO2/fraction of inspired oxygen ratio (p=0.004) and peripheral SO2 measured by pulse oximetry (p< 0.0001) significantly increased soon after HFNC application. Oxygenation improvements were greater after escalation from NIV/CPAP and in patients discharged alive.
Conclusion. HFNC significantly improved oxygenation in severe ARF after failure of COT or NIV/CPAP and may be particularly suitable for older patients hospitalized in non-intensive medical wards.
Prospective Clinical Trials to Advance the Study of Immunotherapy Toxicities
Authors: Cluxton Christopher , Naidoo Jarushka
Published: May 2023

Immune checkpoint inhibitors (ICIs) are a class of drug that produces durable and sustained anti-tumour responses in a wide variety of malignancies. The exponential rise in their use has been mirrored by a rise in immune-related adverse events (irAEs). Knowledge of such toxicities, as well as effective management algorithms for these toxicities, is essential to optimize clinical efficacy and safety. Currently, the guidelines for management of the irAEs are based largely on retrospective studies and case series. In this article, we review the current landscape of clinical trials investigating the management of irAEs with an aim to develop standardized, randomized controlled trial-based management algorithms for ICI-related toxicities.
Dysfunction of Ca2+ Channels in Hereditary and Chronic Cardiovascular Diseases
Authors: Шемарова Ирина Владимировна
Published: May 2023

Cardiovascular diseases (CVD) account for about 17.3 million annual deaths worldwide. 85% of these deaths occurred as a result of myocardial infarction (MI) and stroke. Chronic heart diseases, such as arterial hypertension (AH), coronary heart disease, various cerebrovascular diseases, dilated and hypertrophic cardiomyopathies are widespread, with a fairly high incidence of mortality and disability. Most of these diseases are characterized by cardiac arrhythmias, conduction and contractility disorders. Additionally, interruption of the electrical activity of the heart, the appearance of extensive ectopic foci and heart failure are all symptoms of a number of severe hereditary diseases.

The molecular mechanisms leading to the development of CVD are associated with impaired permeability and excitability of cell membranes and mainly caused by dysfunction of cardiac Ca2+ channels.

Acquired channelopathies are caused by metabolic disorders, increased tone of the sympathetic nervous system, age-related changes that lead to deterioration of coronary blood flow and hemodynamics. Currently known channelopathies, such as the long or short QT syndromes, Brugada and Lenegre syndromes, catecholaminergic polymorphic ventricular tachycardia, etc., are congenital and genetic disorders caused by mutations of genes "responsible" for the conductive properties of certain channel-forming proteins, including pore-forming subunits of Ca2+ channels.

Over the past 50 years, more than 100 varieties of ion channels have been found in the cardiovascular system cells. The relationship between the activity of these channels and cardiac pathology, as well as the general cellular biological function, has been intensively studied on several cell types and experimental animal models in vivo и in situ.

In this review, we discuss the origin of Ca2+ channelopathies and the role of Ca2+ channels of various types: L- R-, T-types voltage-gated calcium channels, RyR2, non-selective hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, transient receptor potential (TRPC, TRPM7, TRPA1) channels in the development of cardio-vascular pathology.
Climate Change Potential Impacts on the Tuna Fisheries in the EEZ of Tonga
Authors: Vaihola Siosaia , Kininmonth Stuart
Published: May 2023

The potential impacts of climate change on the distribution of tuna in Pacific Island Countries’ Exclusive Economic Zones have yet to be investigated rigorously, and so their persistence and abundance in these areas remain uncertain. Here, we estimate optimal fisheries areas for four tuna species; Albacore (Thunnus alalunga), Bigeye (Thunnus obesus), Skipjack (Katsuwonus pelamis), and Yellowfin (Thunnus albacares). We consider different climate change scenarios, RCP 2.6, RCP 4.5, RCP 6.0 and RCP 8.5, within a set of tuna catch records in the Exclusive Economic Zone of Tonga. Using environmental and CPUE datasets, species distribution modelling estimated and predicted these fisheries areas in the current and future climatic scenarios. Our projections indicate an expansion in area and a shift of productive areas to the southern part of this Exclusive Economic Zone of Tonga. This is an indication that future climatic scenarios might be suitable for the species under study however, changes in trophic layers, ocean currents and ocean chemistry might alter this finding. Information provided here will be relevant in planning future national actions towards proper management of these species.
Characteristics of Mental Toughness in Young Basketball Players of Different Age Groups
Authors: Pocius Eimantas , Malinauskas Romualdas
Published: May 2023

Assessing the development of skills that ensure personal mental toughness in adolescence is important because the increasing autonomy and socialization of adolescents during adolescence inevitably creates the conditions for the emergence of an increasing number of external stressors. Paradoxically, adolescents' confidence in their ability to cope with external stressors decreases during adolescence, which may lead to poorer sport performance in adolescent athletes. The aim of this study was to determine and analyze mental toughness skills in the cadet and junior age groups of young basketball players. An additional aim is to reveal how general (total) mental toughness and age predict each of the athletic mental toughness skills. The analysis of the results of the study revealed that players in the junior age group scored statistically significantly higher on the challenge, commitment, emotional control, life control, overall control, self-confidence in one's abilities, self-confidence in interpersonal interactions, overall self-confidence, total mental toughness, determination and visualization scales. Statistically significant correlations were found between mental toughness skills and the different age groups in all scales except positive cogni-tion and self-belief. Total mental toughness was a strong predictor of the athletic mental tough-ness skills, but age did not predict determination and self-beliefs skills.
Penerapan Motif Buketan Kamboja Bali Pada Make Up Fantasi Mitologi Yunani Dewi Alam Baka
Authors: Cyrilla luthfi Azarine
Published: May 2023

Tata rias wajah karakter dengan menyerupai buketan kamboja Bali pada makeup fantasi mitologi yunani dewi alam baka, kamboja bali ini memancarkan aura elegan yang terinspirasi oleh Persephone. tata rias wajah terlihat memiliki aura yang menawan jika memakai desain busana,.sebelum pembuatan desain busana harus dibuat sesuai dengan moodboardnya agar busana dan tata rias waja terlihat sesuai dengan tema maka tata rias tersebut dapat dianalisis sebagai berikut: Center of interest : hal yang ditujukan corak dari Motif Buketan Bali. Focal point

: titik pusat dari riasan terdapat pada penambahan warna merah orange. Harmoni hue : pemilihan warna eyeshadow yang dipilih menggunakan warna yang harmonis,. Axial balance : terdapat keseimbangan corak motif Buketan Bali pada wajah kanan dan kiri, meskipun ukuran pada kedua gambar simetris sama. Rhtym : adanya pengulangan Motif Buketan bali corak - corak garis. Repetition : terdapat pengulangan dari bunga kamboja dan tile.Kamboja makeup Fantasi Motif Buketan Kamboja Bali memancarkan aura elegan dan mitodologi fantasi. Busana memiliki model seperti fantasi dress dan aksesoris millineris seperti headpice yang membuat keseluruhan dari produk ini semakin memperkuat sumber ide persephone sang dewi kematian
PENGETAHUAN MAHASISWA TERHADAP PENGGUNAAN KOSMETIK NON-BPOM DI FAKULTAS PARIWISATA DAN PERHOTELAN UNIVERSITAS NEGERI PADANG
Authors: Nurhasanah  , Siska Yohanifa  , Siska Miga Dewi
Published: May 2023

Penelitian ini di latar belakangi akibat dari persepsi pra-review yang muncul selama pra-studi dan menemukan masalah sehubungan dengan tidak adanya informasi pada mahasiswa di Fakultas Pariwisata dan Perhotelan Universitas Negeri Padang tentang produk perawatan kecantikan non-BPOM. Alasan penelitian ini adalah untuk menentukan derajat informasi mahasiswa Fakultas Pariwisata dan Perhotelan Universitas Negeri Padang. Filosofi eksplorasi yang digunakan dalam penelitian ini adalah korelasional dengan metodologi kuantitatif. Populasi yang diambil dalam penelitian ini adalah mahasiswa Fakultas Pariwisata dan Perhotelan Universitas Negeri Padang BP Tahun 2019 sebanyak 724 orang. Prosedur pemeriksaan yang digunakan adalah strategi pemeriksaan purposive dengan jumlah sampel 82 orang. Strategi pengumpulan informasi menggunakan survei. Metode pemeriksaan informasi dengan mengggunakan SPSS 20. Hasil penelitian menunjukkan pengetahuan mahasiswa tentang kosmetik non-BPOM berada pada kategori tinggi yaitu 85,54 %. Hal ini berarti mahasiswa memiliki pengetahuan yang tinggi mengenai kosmetik non-BPOM di Fakultas Pariwisata dan Perhotelan Universitas Negeri Padang.
KONTRIBUSI HASIL BELAJAR MATA KULIAH KEWIRAUSAHAAN DENGAN MINAT BERWIRAUSAHA DI BIDANG FLORIST PADA MAHASISWA PENDIDIKAN TATA RIAS UNIVERSITAS NEGERI JAKARTA
Authors: Lilies Yulastri  , Silvia Fertyasari  , Lilis Jubaedah  , Lilis Jubaedah
Published: May 2023

Penelitian ini dilatarbelakangi dari kurangnya minat mahasispwa pendidikan tata rias dalam membuka usaha florist. Penelitian ini berutujuan untuk mengetahui seberapa besok kontribusi hasil belajar mata kuliah kewirausahaan terhadap minat berwirausaha di bidang florist pada mahasiswa pendidikam tata rias. Jenis penelitian ini adalah penelitian deskriptif dengan pendekatan korelasional dengan jumlah populasi 36 orang mahasiswa Pendidikan Tata Rias Universitas Negeri Jakarta angkatan 2018. Teknik penarikan sampel dalam penelitian ini menggunakan total population sampling. Data yang digunakan berupa data primer dan sekunder. Instrumen penelitian ini berupa angket dengan menggunakan skala likert yang terdiri dari 5 pilihan jawban. Uji persyaratan analisis. Uji persyaratan analisis dalam penelitian ini menggunakan uji Kolmogorov Smirnov yang menunjukan nilai probabilitas (sig) sebesar 0,070 > 0,05 taraf signifikan, sehingga kedua variabel dapat dinyatakan berdistribusi normal dan nilai linieritas memiliki nilai signifikan deviation from linierity sebesar 0,800 > 0,05. Pengujian hipotesis menggunakan uji regresi linier sederhana, hasil menunjukan r hitung sebesar memiliki nilai signifikan deviation from linierity sebesar 0,215 yang artinya kurang dari r tabel yaitu 0,3291, yang berarti Ha ditolak Ho diterima. Nilai korelasi sebesar 0.215 dan besarnya pengaruh variabel terikat sebesar 0,046. Disimpulakan bahwa kontribusi variabel bebas terhadap variabel terikat sebesar 4,6%, sedangkan sisanya dipengaruhi oleh variabel lain.

 

Kata kunci: Kontribusi, Mata Kuliah Kewirausahaan, Berwirausaha, Florist
Reputasi KAP dalam Memoderasi Pengaruh Ukuran Perusahaan, Profitabilitas, Solvabilitas dan Likuiditas Terhadap Audit Report Lag
Authors: Ma’sumah Lulu’il , Rusyida Wilda Yulia
Published: May 2023

This research was conducted to determine the effect of company size, profitability, solvency, and liquidity on audit report lag with the reputation of public accounting firms as a moderating variable in state-owned companies listed on the Indonesia Stock Exchange for the 2016-2020 period. This type of research is quantitative research. The data used secondary data using purposive sampling. This study conducted descriptive analysis tests, classical assumption tests, and hypothesis tests consisting of the coefficient of determination, t-test, and Moderated Regression Analysis (MRA). The results of this study show that company size, profitability, and liquidity affect the audit report lag companies. In contrast, solvency has no effect. The reputation of the public accounting firm cannot moderate the relationship between firm size, profitability, solvency, and liquidity in the audit report.
Persepsi Mahasiswa Akuntansi Non-Muslim Terhadap Mata Kuliah Akuntansi Syariah di Universitas Tadulako
Authors: Yulianti Yulianti , Muliati Muliati , Jurana Jurana , Halwi Muhammad Darma , Yamin Nina Yusnita , Mustamin Mustamin
Published: May 2023

The research aims to determine non-Muslim accounting students' perception of Sharia accounting courses. This study examines four dimensions: knowledge, experience, methods, and motivation. The data were analyzed using a qualitative descriptive method, and the data source was obtained from interviews. This study had six informants: five non-Muslim accounting students and one accounting lecturer. The research finding shows that from the knowledge dimensions, namely knowing the transparency of sellers and buyers (murabahah contracts), a different source of income, differences in PSAK. The second dimension of experience is that non-muslim accounting students are comfortable teaching lecturers, get benefits, and positively respond to Sharia accounting. The third dimension is the learning method provided, which accounting students like the discussion method during learning. The fourth dimension is motivation to work in Islamic banks and motivated by non-Muslim lecturers who research Islamic accounting.
Competências e habilidades na BNCC: análise reflexiva de uma prática pedagógica produzida e aplicada em uma escola da rede privada do município de Conceição das Alagoas/MG
Authors: Matias Layane , Fernandes Aned
Published: May 2023

O presente trabalho propõe uma análise reflexiva das limitações dos conceitos de habilidades e competências propostos pela Base Nacional Comum Curricular ao trabalho docente. Tal análise é feita a partir de uma prática pedagógica aplicada à uma turma de 8º ano do ensino fundamental da rede particular de ensino do município de Conceição das Alagoas/MG. O objetivo da prática pedagógica aplicada foi avaliar se os conceitos referentes à BNCC podem, de alguma forma, limitar o trabalho docente. Diante disso, o processo de construção do plano de aula foi descrito e relatado no texto, a fim de promover uma melhor visualização dos processos práticos pedagógicos adotados. Apesar de a BNCC ser um documento vigente, é possível perceber que em seu processo de elaboração não houve efetiva participação dos professores e que isso acarreta um desconhecimento acerca de quais são as reais intenções do documento. Com isso, o docente desenvolve suas práticas a partir das vivencias e realidade do aluno, não considerando como referência as habilidades e competências da BNCC.
Intrusion Detection System and Fuzzy Ant Colony Optimization based Secured Routing in Wireless Sensor Networks
Authors: Subramani Shalini , M Selvi
Published: May 2023

Recent advances in Wireless Sensor Networks (WSNs) have brought the sensor based monitoring developments to the surface in many applications. In such a scenario, the security of communication is a major challenge in the effective delivery of the collected data due to the presence of malicious nodes. Moreover, since security and minimization of energy consumption are critical factors in designing techniques for multi-hop secure routing in wireless sensor networks, it is necessary to address the issues of security in the routing process. Hence, this paper proposes a novel intrusion detection system for enhancing the security and Fuzzy based Ant Colony Optimization based Secured Quality of Service Routing Protocol (F-ACO-SQoSRP) for increasing the security of communication and network performance in WSNs. Using this proposed Intrusion Detection System (IDS), the proposed model identifies the distinct and malicious behaviours of nodes. Additionally, a clustering algorithm has been proposed in this work, wherein the Cluster Head Selection (CHS) is based on Quality of Service (QoS) measures and the trust values of nodes are measured using the intrusion detection system results. The proposed ACO based routing framework also predicts the best optimum and secured path to allow for effective communication across each link. The simulation results obtained from this work proved that the proposed secured routing algorithm provides better performance in terms of security using robust trust values, increase in packet delivery ratio and network lifetime reduction in delay and energy consumption when this work is compared with the existing secured routing systems.
Feasibility study of Glucagon-like peptide-1 analogues for the optimization of Outcomes in Obese patients undergoing AbLation for Atrial Fibrillation (GOAL-AF)
Authors: Win Kyaw Zaw , Armstrong Matthew , Steeds Richard P , Kalla Manish
Published: May 2023

Background

Catheter ablation for atrial fibrillation is recommended for symptomatic patients after failed medical therapy. Ablation has a higher failure rate in obese patients, and both the prevalence of atrial fibrillation and obesity are increasing globally. The outcome of ablation can be improved if obese patients can achieve goal-oriented weight reduction prior to ablation. Conventional weight loss strategies however, can be difficult to access and can delay ablation, thereby risking a lower chance of maintaining sinus rhythm. Effective weight-loss medications, such as the glucagon-like peptide inhibitor-1 drugs, offer the potential for incremental impact on weight loss over a shorter period of time as a bridging therapy. The aim of this study is to assess the feasibility of using liraglutide, a glucagon-like peptide inhibitor-1, in producing weight loss in obese patients before catheter ablation.

Methods

The study is an open label, uncontrolled, prospective single centre feasibility study of daily liraglutide injections in the treatment of obese patients for at least 13 weeks before and 52 weeks after AF ablation. Adult patients with symptomatic AF whose body mass index ≥30 will be recruited from those planning to undergo ablation. Feasibility will be determined based on the recruitment rate, adherence to the medication and the amount of weight loss achieved over the study period. Exploratory outcomes include changes in atrial structure, function and fibrosis with weight loss evaluated by cardiac magnetic resonance imaging, electroanatomic mapping, and patient reported outcome measure.

Discussion

This study will allow us to determine whether the use of liraglutide in obese patients with atrial fibrillation undergoing ablation is feasible with adequate recruitment. The additional information on adherence and average weight loss over the study period will inform the design of a future definitive randomized controlled trial.

Trial registration

ClinicalTrials.gov (NCT05221229)
Registered on 2 February 2022 (https://clinicaltrials.gov/ct2/show/NCT05221229)

Trial funding

Metchley Park Medical Society and University of Birmingham Starter Fellowship, British Heart Foundation Accelerator Grant, Abbott Investigator Initiated Study Grant.
Demand Factors that Influence Local Furniture Marketing in Ghana: A Case Study of the Kumasi Metropolis, Ghana
Authors: Agyeman Justice Duah , Boateng Ransford Kwasi , Inyong Stephen , Antwi-Boasiako Charles , Ewudzie Job
Published: May 2023

The contribution of the furniture sector to economic development remains unabated in developing economies, Ghana however, is yet to fully experience the fair share of economic contribution of this industry to her Gross Domestic Product. The study examined the demand factors that influence local furniture marketing and the relative contribution of these factors to the marketing of local furniture. A total of 132 and 169 furniture producers and consumers respectively were selected through cluster and simple random sampling and accidental sampling respectively with a questionnaire-return rate of 82.6% and 87% each. Quantitative data were gathered through questionnaires and analyzed using 20.0 SPSS analysis Software Package. The Relative Importance Index (RII) was used to rank the demand factors and their relative contribution to local furniture marketing. The use of good quality aesthetic recorded 3.68 and that of door-to-door marketing of local furniture marketing (3.91) were the chief factors that accounted for high demand for local furniture marketing. Production of simple furniture that reflect the social status of consumers and rigorous product development and promotions recorded (4.62) and accounted for the relative contribution of the demand factors for local furniture marketing. Local furniture marketing would improve if designs reflected the social status of consumers, and producers invested in product development and promotions. In order to understand consumer demands producers would have to tailor production towards consumer demands so that local furniture would be attractive and sales and marketing would increase.
Conditions for adherence to videoconference-based programs promoting adapted physical activity in cancer patients: a realist evaluation
Authors: AROMATARIO Olivier , Cambon Linda , Alla François , Imbert Alexis , Pouchepadass Camille , Renvoisé Nathalie , Dauchy Sarah , Charles Cécile
Published: May 2023

Background
Although moderate physical activity (PA) during cancer treatment has been associated with improved quality of life, reduced side effects, and even lower mortality, many barriers to successful implementation remain. Digital technology has been perceived as an effective lever for overcoming access and motivational issues – the exponential use of videoconferencing during the Covid-19 pandemic has reinforced this promise – but few studies have been performed to confirm this assumption. The "VISIO-AJUST" study explored the factors affecting the conditions of adherence to video-conference-based PA programs in patients undergoing cancer treatment.
Methods
The VISIO-AJUST study was based on a qualitative successive case approach, guided by the principles of the realist evaluation, and applied to two French programs of PA, following three main steps: 1) Identification of factors likely to influence conditions of patients’ adherence; 2) Elaboration and testing of explanatory “Context-Mechanism-Outcome (CMO)” configurations; 3) Refinement of CMO configurations, in order to understand what, how, for whom, and under what circumstances video-conference-based PA programs work.
Results
Five main CMO configurations were found to be associated with adherence to video-conferencing-based PA programs, promoting (i) Accessibility and a supportive environment for adapted physical activity, (ii) A setting conducive to sociability despite distance, (iii) Confidence and security of practice, (iv) A combination of several motivational levers in favor of continuity of effort and progress, (v) Regularity of the sessions, progressiveness in the effort and evaluation of progress as a basis for the adoption of a regular physical practice.
Conclusion
This study provides original insights into the use of videoconferencing to enable patients to improve their PA during cancer treatment. Future research with long-term follow-up would allow for a better understanding of the key conditions promoting sustainable behavior change.
SRSF3/AMOTL1 Splicing Axis Promotes the Tumorigenesis of Nasopharyngeal Carcinoma through Regulating the Nucleus Translocation of YAP1
Authors: Xu Xiao-Chen , Jiang Jiaxin , Zhou Ya-Qing , He Shuai , Liu Yang , Wei Pan-Pan , Bei Jin-Xin , Sun Jian , Luo Chun-Ling
Published: May 2023

Dysregulation of serine/arginine splicing factors (SRSFs) and thereby the abnormal alternative splicing (AS) has been widely implicated in the development of multiple cancers, but scarcely investigated in nasopharyngeal carcinoma (NPC). Here we examine the expression of 12 classical SRSFs between 87 NPC and 10 control samples, revealing a significant upregulation of SRSF3 and its association with worse prognosis in NPC. Functional assays demonstrate that SRSF3 exerts oncogenic function in NPC progression. Transcriptome analysis reveals 1 934 SRSF3-regulated AS events in genes involved in cell cycle and mRNA metabolism, among which generation of a long isoform of AMOTL1 (AMOTL1-L) is further verified through a direct bond of SRSF3 RRM domain with exon 12 of AMOTL1 to promote exon inclusion. Functional studies reveal that AMOTL1-L promotes proliferation and migration of NPC cells, while AMOTL1-S does not. Moreover, overexpression of AMOTL1-L significantly rescues the abovementioned inhibitory effects of SRSF3 knockdown, but not AMOTL1-S. Furthermore, compared with AMOTL1-S, AMOTL1-L has a localization preference in the intracellular than cell membrane, leading to a stronger interaction with YAP1 to promote its translocation to nucleus. Taken together, our findings identify SRSF3/AMOTL1 as a novel alternative splicing axis with pivotal roles in NPC development, which could be served as promising prognostic biomarkers and therapeutic targets of NPC.
Validation of an improved reference freeze-dried direct agglutination test for the detection of leishmaniasis in the canine reservoir
Authors: Harith Abdallah el , Abass Elfadil , Martinkovic Franjo , Mansour Durria , Osman Hussam
Published: May 2023

Introduction: Proper identification and management of reservoir post kala-azar dermal leishmaniasis (PKDL) and canine visceral leishmaniasis (CVL) cases are prerequisites to the effective control of visceral leishmaniasis (VL) world-wide. Unlike PKDL, CVL still awaits effective improvement because of its cryptic nature, absence of Leishmania parasite in lesions or lymph-nodes and insensitivity of tools in current use. Because of the need for certain skill and equipment, both the liquid direct agglutination test (LQ-DAT) and freeze-dried direct agglutination test (FD-DAT) versions, present in comparison with the indirect immunofluorence (IFAT) or enzyme-linked immunosorbant assay (ELISA), practical and feasible diagnostic alternatives. Due to difficulties in local production or high application cost involved, routine application of direct agglutination test (DAT) was greatly hampered in major CVL endemic areas.
Aim: Validate performance of an improved FD-DAT to suit routine and large scale application in CVL endemic areas.
Methods: Introducing of citrate-saline formaldehyde (CSF) as anti-clumping agent to replace normal saline for antigen reconstitution and drastically however eligibly lowering concentration of promastigotes (1.4X107) in comparison with the original reference FD-DAT (> 5X107/ml), To ensure optimal safety, β-mercaptoethanol (β-ME) was replaced by urea or sodium dodecyle sulphate (SDS) as a serum reducing agent.
Results: Through improving the procedure for reconstitution of FD-DAT antigen with CSF a 150% reduction in the test application cost was achieved. Expired test batches (+ 4 years earlier), were successfully revitalized to full validity. As compared to 48-hour shelf-life time for the original, an FD-DAT batch re-constituted here with CSF maintained validity for + 12 months.
Conclusion: The highly concordant results with IFAT and ELISA (One Way ANOVA Test P = 0.142, Homogeneity of Variances P = 0.009) as routine CVL diagnostics further motivate application of the improved FD-DAT for detection of the disease in endemic areas.
An Evaluation of the Cost of Human Papilloma Virus (HPV) Vaccine Delivery In Zambia
Authors: Simuyemba Moses C , Chama-Chiliba Chitalu M , Chompola Abson , Sinyangwe Aaaron , Bchir Abdallah , Asiimwe Gilbert , Masiye Felix , Chibwesha Carla
Published: May 2023

Background
Human papillomavirus (HPV) is a common sexually transmitted infection and the leading cause of cervical cancer. The HPV vaccine is a safe and effective way to prevent HPV infection. In Zambia, the vaccine is given during Child Health to girls aged 14 years who are in and out of school in two doses over two years. The focus of this evaluation was to establish the cost to administer a single dose of the vaccine well as for full immunisation of two doses.
Methods
For HPV costing, both top-down and micro-costing approaches were used, depending on the cost data source, and economic costs were gathered from Expanded Programme for Immunisation Costing and Financing Project (EPIC). Data was collected from eight districts in four provinces, mainly using a structured questionnaire, document reviews and key informant interviews with staff at national, district and provincial levels.
Results
Findings show that schools made up 53.3% of vaccination sites, community outreach sites 30.9% and finally health facilities 15.8%. In terms of coverage for 2020, for the eight districts sampled, schools had the highest coverage at 96.0%. Community outreach sites were at 6.0% of the coverage and health facilities accounted for only 1.0% of the coverage. School based delivery had the lowest cost economic cost at USD13.2 per dose and USD 26.4 per fully immunised child (FIC). Overall financial costs were US$6.0 per dose and US$11.9 per fully immunised child. Overall economic costs taking all delivery models into account were US$23.0 per dose and US$46.0 per FIC. The main cost drivers were human resources, building overhead and vehicles, microplanning, supplies and service delivery/outreach. were the top cost drivers. Nurses, environmental health technicians and community-based volunteers were the most involved in HPV vaccination.
Conclusions
Future planning in Zambia and other African countries conducting HPV vaccination needs to prioritise these cost drivers as well as possibly find strategies to minimise some costs. Although not a challenge now due to Gavi support, vaccine costs are a major threat to sustainability in the long run. Countries like Zambia must find strategies to mitigate against this.
One Pot Multicomponent Synthesis of Highly Substituted Imidazoles Using Tetrabutylammonium Peroxy disulfate as a Catalyst
Authors: Nukala Ramesh , Gullapelli Kumaraswamy , Konakanchi Ramaiah
Published: May 2023

Tetra butyl ammonium Peroxy disulfate has been found to be an efficient and rapid medium for thesynthesis of novel substituted imidazoles. The current investigation involving different mole ratio of catalyst and different solvents were used. The employed strategy/methodology is efficient, economic and sustainable owing to its distinctive characteristics such as eco-friendly low reaction times, excellent yields, cost-effective due to avoid the use of toxic solvents and reusability of the catalyst, simple workup procedure, and extensive applicability. Results reveal that the 20% mole ratio and acetonitrile gave excellent yield. Structures of newly synthesized compounds have been confirmed by Proton NMR, 13CNMR, IR and mass spectra.
Association between cardiovascular health metrics and restless legs syndrome: A population-based study
Authors: Wang Dongxiao , Lin Jianping , Li Qingxia , Ma Xiuxia , Zhang Xuetong , Luo Shan , Liao Renfeng
Published: May 2023

Background Cardiovascular diseases is increasingly identified to be related to the restless legs syndrome (RLS). However, the relationship between Cardiovascular Health Metric (CVH) and RLS need to be further confirmed. The present study aimed to assess the association of overall CVH metric and 7 Simple’s Life (LS7) with the RLS risk.
Methods In a cross-sectional population-based study, 3,772 adults (57.6 ± 5.11 years of age) were recruited and completed the structured questionnaire between January 2 and May 21, 2022. Blood sample and other body measurements were obtained by trained nurses. The definition and score of CVH metric was determined by the attendance of LS7, and the RLS was diagnosed by the International Restless Legs Syndrome Study Group (IRLSSG) criteria. Multivariate logistic regression models were applied to examine the associations of overall CVH metric and its SL7 profiles with prevalence of RLS.
Results Overall, 301 (7.98%) were diagnosed with RLS. Multivariable logistic regression analysis showed that, in comparison to inadequate scores, higher scores of overall CVH metric and body mass index (BMI), physical activity, blood pressure and total cholesterol (TC) metric were negatively associated with the prevalence of RLS (multi-adjusted odd ratios [ORs] ranged from 0.32 to 0.67, all P for trend < 0.05). Per 1-SD increase in over CVH metric and each SL7 profile yielded the similar results (ORs ranged from 0.65 to 0.85, all P for trend < 0.05). Significant differences in the association of RLS with smoke profile and overall CVH metric were detected with females (P for interaction = 0.005) and older participants (P for interaction = 0.013), respectively.
Conclusion To be at an ideal behavioural CVH may be benefit in RLS, especially for women and older people. Interventions concerning to promote and preserve favourable CVH should be regarded in the prevention and treatment of RLS.
The effect of ultrasonic rolling on the fatigue performance of laser-welded TC4 titanium alloy joints
Authors: Jiahui Cong , Jiayuan Gao , Song Zhou , Zhichao Zhang , Jiahao Wang , Naijing Wang
Published: May 2023

In order to improve the fatigue performance of TC4 titanium alloy laser welded joints, ultrasonic rolling treatment was adopted in this study, and different passes of rolling process were used. The research results show that the ultrasonic rolling treatment significantly improves the fatigue limit and fatigue life of the weldment. At room temperature, the fatigue strength of the weldment increases by 2.04–4.58%, and the corrosion fatigue life increases by 1.71 to 3.05 times. This study also deeply analyzed the significant effects of ultrasonic rolling treatment on surface morphology, microstructure, surface residual stress and microhardness to reveal its strengthening mechanism. It was found that the ultrasonic rolling treatment shifted the crack initiation point to the subsurface and formed a hard layer with high residual stress on the surface by applying significant static pressure input and multiple treatments. This change makes the fatigue stripes narrower and denser. Compared with the traditional weld surface treatment method, ultrasonic rolling treatment significantly improves the surface quality and fatigue performance of TC4 titanium alloy laser welded joints.
Evaluating knowledge, Attitudes, and Practices Regarding Interruption of Enteral Nutrition in ICU Medical staff:A descriptive cross-sectional survey
Authors: Yuanyuan Mi , fei Tian , Chao Zeng , Liu Jing , Haiyan Huang
Published: May 2023

Objectives: Investigate the status of knowledge, attitude, and practice(KAPs) on Interruption of enteral feeding among ICU medical staff, and explore influencing factors of KAPs score.
Design: questionnaires including general information as well as knowledge, attitude, and practice(KAPs) on Interruption of enteral feeding were distributed in November 2021 to 575 ICU medical staff. t-test and logistic and linear regressions were used to assess Status of interruption of enteral feeding and relevant influencing factors.
Setting: Hospitals in Hubei Province(n =14).
Participants: A total of 575 ICU medical staff including: nurses(n =401); doctors(n =153); all staff responded and 554 were included in the analysis.
Results: of the 554 participating ICU medical staff, the median score in knowledge, attitude and practice was 55(47, 60), 50(44, 50) ,73(67, 80),respectively. Having an advanced degree((bachelor's degree or above,p=0.007), the high grade hospital(Class III A, p =0.020), undertaking a more comprehensive training(Learned but not comprehensive,p=0.037;systematic learning, p <0.001), Main learning approaches(Books and Internet; p =0.029), the frequency in academic journals(often, p =0.005) was the contributing factor to score of total KAPs; undertaking a comprehensive the training(Systematic learning, p <0.001), High frequency in Academic journals(sometimes, p=0.008; often, p<0.001; always, p =0.032) was the contributing factor to score of knowledge dimension; Having an advanced degree(bachelor's degree or above, p =0.004) was the socre of attitude dimension; Having an Advanced degree(bachelor's degree or above, p =0.026) and undertaking a comprehensive the training(systematic learning, p =0.013) was the contributing factor to score of parctice dimension; There was a significant positive correlation between the total score and score of knowledge, attitude and practice (p <0.05).
Conclusions: Based on the findings, the hospital management department should formulate targeted training programs to further improve the level of enteral nutrition interruption among ICU medical staff.
Bacterial Isolates and Characteristics of Children With Febrile Neutropenia on Treatment for Cancer at Moi Teaching and Referral Hospital, Kenya.
Authors: Kipchumba Samuel , Njuguna Festus M. , Nyandiko Winstone M.
Published: May 2023

Purpose
This study aimed at identifying the patient characteristics of children with febrile neutropenia, the associated bacterial organisms, and their sensitivity patterns.
Methods
A descriptive cross-sectional study was carried out at the MTRH, paediatric oncology ward, from June 2021 to April 2022. A total of 110 children who developed fever and neutropenia while on chemotherapy were enrolled. Blood samples for culture were drawn aseptically. Patient characteristics were presented in frequency tables. Antimicrobial sensitivity patterns were plotted in tables against the bacterial isolates cultured. Chi-square/Fischer’s exact test were used to determine any association between patient characteristics, bacterial growth and antimicrobial sensitivity.
Results
Majority 66 (60%) were males. The median age was 6.3 (SD 3.7) years. Majority of the patients 71 (64.5%) had haematological malignancies, the most common being Acute Myeloid Leukaemia (AML). There was a significant association between severity of neutropenia and haematological malignancies p = 0.028. In total, 31/110 (28.2%) blood cultures were positive for bacterial growth. Gram-positive bacteria were more frequent at 20 (58.1%). The most common organism was Escherichia coli 6 (18.2%), followed by Staphylococcus aureus at 5 (15.2%.) All the isolates were sensitive to linezolid and vancomycin and also showed good sensitivity towards meropenem at 10/11 (90.9%). High resistance to cephalosporins was noted with ceftriaxone at 5/6 (83.3%), cefepime at 4/7 (57.1%) and ceftazidime at 3/4 (75%).
Conclusion
The most common malignancy associated with febrile neutropenia was AML. Gram-positive bacteria were the most common isolates. There was high resistance towards cephalosporins but all were sensitive to linezolid and vancomycin.
Multi-directional neutron dark-field imaging with single absorption grating
Authors: Busi Matteo , Shen Jiazhou , Bacak Michael , Zdora Marie , Valsecchi Jacopo , Strobl Markus
Published: May 2023

Neutron dark-field imaging is a powerful technique for investigating the microstructural properties of materials through high-resolution full-field mapping of small-angle scattering. However, conventional neutron dark-field imaging utilizing Talbot-Lau interferometers is limited to probing only one scattering direction at a time. Here, we introduce a novel multi-directional neutron dark-field imaging approach that utilizes a single absorption grating with a two-dimensional pattern to simultaneously probe multiple scattering directions. The method is demonstrated to successfully resolve fiber orientations in a carbon compound material as well as the complex morphology of the transformed martensitic phase in additively manufactured stainless steel dogbone samples after mechanical deformation. The latter results reveal a preferential alignment of transformed domains parallel to the load direction, which is verified by EBSD. The measured real-space correlation functions are in good agreement with those extracted from the EBSD map. Our results demonstrate that multi-directional neutron dark-field imaging is overcoming significant limitations of conventional neutron dark-field imaging in assessing complex heterogeneous anisotropic microstructures and providing quantitative structural information on multiple length scales.
mm-Wave Radar-Based Multiple Patients’ Breathing and Heart Rates Measurement
Authors: Ahmed Sajid , Abdullah Muhammad , Alouini Mohamed-Slim
Published: May 2023

This paper demonstrates an mm-wave frequency-modulated continuous-wave (FMCW) radar to simultaneously measure multiple stationary patients’ breathing and heart rates. The proposed algorithm cancels the background interference and applies fast-Fourier-transform on fast-time samples to measure the location and on slow-time samples to measure the breathing and heart rates. The algorithm is implemented on a commercially available Infineon’s "Position2Go" module, which is one transmit and two receive antenna FMCW radar and operates at 24GHz. In contrast to the reported systems, our inexpensive system can reliably measure multiple patients’ vital signs and localize them in range. The proposed system can find applications in the healthcare system by offering a centralized inexpensive wireless vital sign monitoring of multiple patients without fixing any probe on the patient’s body.
Transparent, Mechanically Robust, Low-temperature- tolerance, and Stretchable Ionogels Enhanced by Konjac Glucomannan toward Wireless Strain Sensors
Authors: Ye Zhifan , Zheng Yijia , Jia Qihan , Wang Haibo , Xiong Junjie , Wang Shuang
Published: May 2023

Electronic skins (E-skins) are capable of detecting human health and movement, which has promising potential in the fields of human-machine interactions and artificial intelligence. However, traditional hydrogels-based E-skins suffer from poor mechanical strength, low conductivity, and instability owing to the evaporation of water. Herein, to advance the unsatisfaction of E-skins above, a semi-interpenetrating network developed by polysaccharide biomass konjac glucomannan (KGM) was introduced into covalent-crosslinked network (PAA-co-PAM) to synthesize a transparent, tough, nonvolatile, and highly stretchable ionogel with an ionic liquid (EMIM:DCA) as conductive media. This ionogel exhibited extraordinary mechanical strength (tensile strength 2.77 MPa), outstanding mechanically durability (100 stretching cycles of 250%) and elongation (elongation at break 997%). More importantly, the ionogel demonstrated remarkable anti-freezing performance (high flexibility at -20℃) and high conductivity (3.94 mS/cm) in the absence of water. Besides, after assembly from konjac glucomannan-enhanced ionogel, the sensor exhibited comprehensive strain sensing performance, which could effectively and accurately monitor the human motion via Bluetooth transmission. This strategy paves the way for a viable new generation of multifunctional biomimetic super-sensitive sensors, which have great promise for applications such as intelligent devices, health detection, and biomedical monitoring in harsh conditions.
WITHDRAWN: Assessment of statistical and machine learning methods for landslide susceptibility of Pithoragarh, Uttarakhand (India)
Authors: Bhardwaj Vanshika , Singh Kanwarpreet
Published: May 2023

Landslides are the most prevalent natural hazard in hilly regions of India. These can have a significant impact on the economy of a nation. This study examines the landslide susceptibility of the Pithoragarh, Uttarakhand, India, utilising various susceptibility methods, such as Frequency Ratio (FR), Information Value (IV), Weight of Evidence (WOE), and Certainty Factor (CF). The LSZ modelling was performed using fourteen landslide causative factors. Based on past landslide data, landslide locations were identified, which were further divided into a 70/30 ratio, with 70 representing training and 30 representing validation. Validation of the findings of the predicted maps of landslide susceptibility using Area under Curve (AUC) indicates that the predicted map using the FR approach has the highest prediction rate compared to other methods used for landslide susceptibility prediction. Also to check the feasibility of the machine learning method, we have considered logistic regression (LR) analysis using five out of fourteen factors. AUC revealed that LR has higher accuracy than Analytical Hierarchy Process (AHP) and Shannon Entropy (SE). Also, validation of all the models was done using Landslide Density Index (LDI) which shows the validity of all models. Thus, the results of all models can be used to predict landslide susceptibility in Pithoragarh.