bluuebunny/crossref_metadata_2025 huggingface dataset:
Zebrafish Patient-Derived Xenograft Model to Predict Treatment Outcomes of Colorectal Cancer Patients
Authors: Di Franco Gregorio , Usai Alice , Piccardi Margherita , Cateni Perla , Palmeri Matteo , Pollina Luca Emanuele , Gaeta Raffaele , Marmorino Federica , Cremolini Chiara , Dente Luciana , Massolo Alessandro , Raffa Vittoria , Morelli Luca
Published: June 2022

The use of zebrafish embryos for personalized medicine has become increasingly popular. We present a co-clinical trial aiming to evaluate the use of zPDX (zebrafish Patient-Derived Xenografts) in predicting the response to chemotherapy regimens used for colorectal cancer patients. zPDXs are generated by xenografting tumor tissues in two days post-fertilization zebrafish embryos. zPDXs were exposed to chemotherapy regimens (5-FU, FOLFIRI, FOLFOX, FOLFOXIRI) for 48 h. We used a linear mixed effect model to evaluate the zPDX-specific response to treatments showing for 4/36 zPDXs (11%), a statistically significant reduction of tumor size compared to controls. We used the RECIST criteria to compare the outcome of each patient after chemotherapy with the objective response of its own zPDX model. Of the 36 patients enrolled, 8 metastatic colorectal cancer (mCRC), response rate after first-line therapy, and the zPDX chemosensitivity profile were available. Of eight mCRC patients, five achieved a partial response and three had a stable disease. In 6/8 (75%) we registered a concordance between the response of the patient and the outcomes reported in the corresponding zPDX. Our results provide evidence that the zPDX model can reflect the outcome in mCRC patients, opening a new frontier to personalized medicine.
Genetic Polymorphisms in the 3′-Untranslated Regions of SMAD5, FN3KRP, and RUNX-1 Are Associated with Recurrent Pregnancy Loss
Authors: Kwon Min-Jung , Kim Ji-Hyang , Lee Jeong-Yong , Ko Eun-Ju , Park Hyeon-Woo , Shin Ji-Eun , Ahn Eun-Hee , Kim Nam-Keun
Published: June 2022

Recurrent pregnancy loss (RPL) is typically defined as two or more consecutive pregnancy losses prior to 20 weeks of gestation. Although the causes of idiopathic RPL are not completely understood, vascular development and glucose concentration were reported to correlate with the pregnancy loss. The TGF-β signaling pathway which plays a significant role in pregnancy is activated by the interaction between high glucose and SMAD signaling and affects the vascular cells. SMAD5 and RUNX-1 are involved in the TGF-β signaling pathway and contribute to advanced glycation end products (AGEs) production and vascular development. FN3KRP, a newly described gene, is also associated with vascular diseases and suggested to relate to AGEs. Therefore, in the present study, we investigated associations between RPL risk and genetic polymorphisms of SMAD5, FN3KRP, and RUNX-1 in 388 women with RPL and 280 healthy control women of Korean ethnicity. Participants were genotyped using real-time polymerase chain reaction and restriction fragment length polymorphism assay to determine the frequency of SMAD5 rs10515478 C>G, FN3KRP rs1046875 G>A, and RUNX-1 rs15285 G>A polymorphisms. We found that women with RPL had lower likelihoods of the FN3KRP rs1046875 AA genotype (adjusted odds ratio (AOR), 0.553; p = 0.010) and recessive model (AOR, 0.631; p = 0.017). Furthermore, combination analysis showed that SMAD5 rs10515478 C>G and FN3KRP rs1046875 G>A mutant alleles were together associated with reduced RPL risk. These findings suggest that the FN3KRP rs1046875 G>A polymorphism has a significant role on the prevalence of RPL in Korean women. Considering that it is the first study indicating a significant association between FN3KRP and pregnancy disease, RPL, our results suggest the need for further investigation of the role of FN3KRP in pregnancy loss.
Advances in Detection of Antibiotic Pollutants in Aqueous Media Using Molecular Imprinting Technique—A Review
Authors: Ayankojo Akinrinade George , Reut Jekaterina , Nguyen Vu Bao Chau , Boroznjak Roman , Syritski Vitali
Published: June 2022

Antibiotics constitute one of the emerging categories of persistent organic pollutants, characterised by their expansion of resistant pathogens. Antibiotic pollutants create a major public health challenge, with already identifiable detrimental effects on human and animal health. A fundamental aspect of controlling and preventing the spread of pollutants is the continuous screening and monitoring of environmental samples. Molecular imprinting is a state-of-the-art technique for designing robust biomimetic receptors called molecularly imprinted polymers (MIPs), which mimic natural biomolecules in target-selective recognition. When integrated with an appropriate sensor transducer, MIP demonstrates a potential for the needed environmental monitoring, thus justifying the observed rise in interest in this field of research. This review examines scientific interventions within the last decade on the determination of antibiotic water pollutants using MIP receptors interfaced with label-free sensing platforms, with an expanded focus on optical, piezoelectric, and electrochemical systems. Following these, the review evaluates the analytical performance of outstanding MIP-based sensors for environmentally significant antibiotics, while highlighting the importance of computational chemistry in functional monomer selection and the strategies for signal amplification and performance improvement. Lastly, the review points out the future trends in antibiotic MIP research, as it transits from a proof of concept to the much demanded commercially available entity.
ERK1/2 Signalling Pathway Regulates Tubulin-Binding Cofactor B Expression and Affects Astrocyte Process Formation after Acute Foetal Alcohol Exposure
Authors: Zheng Yin , Huo Jiechao , Yang Mei , Zhang Gaoli , Wan Shanshan , Chen Xiaoqiao , Zhang Bingqiu , Liu Hui
Published: June 2022

Foetal alcohol spectrum disorders (FASDs) are a spectrum of neurological disorders whose neurological symptoms, besides the neuronal damage caused by alcohol, may also be associated with neuroglial damage. Tubulin-binding cofactor B (TBCB) may be involved in the pathogenesis of FASD. To understand the mechanism and provide new insights into the pathogenesis of FASD, acute foetal alcohol exposure model on astrocytes was established and the interference experiments were carried out. First, after alcohol exposure, the nascent astrocyte processes were reduced or lost, accompanied by the absence of TBCB expression and the disruption of microtubules (MTs) in processes. Subsequently, TBCB was silenced with siRNA. It was severely reduced or lost in nascent astrocyte processes, with a dramatic reduction in astrocyte processes, indicating that TBCB plays a vital role in astrocyte process formation. Finally, the regulating mechanism was studied and it was found that the extracellular signal-regulated protease 1/2 (ERK1/2) signalling pathway was one of the main pathways regulating TBCB expression in astrocytes after alcohol injury. In summary, after acute foetal alcohol exposure, the decreased TBCB in nascent astrocyte processes, regulated by the ERK1/2 signalling pathway, was the main factor leading to the disorder of astrocyte process formation, which could contribute to the neurological symptoms of FASD.
Evaluation of the Efficacy of a New Dichoptic Digital Platform to Treat the Anisometropic and Isometropic Amblyopia
Authors: Abdal Md Oliullah , Bhombal Faiza , Nankani Gul J. , Nankani Sonia G. , Lad Shruti , Dholam Aditi , Kumari Richa , Mahajan Jinal , Piñero David P.
Published: June 2022

The aim of the current study was to evaluate the results of a novel dichoptic training program using an online platform in a group of subjects with refractive amblyopia, performing a comparative analysis of unilateral and bilateral amblyopic cases. For this purpose, a retrospective study analysis of data of 161 children (4–13 years) who underwent dichoptic treatment with the Bynocs® platform (Kanohi Eye Pvt. Ltd., Mumbai, India) was performed. In all cases, the therapy protocol consisted of sessions of training of 30 min daily 5 times a week for 6 weeks. Best corrected visual acuity (BCVA) in the non-dominant eye improved significantly with the treatment, with a mean change of 0.39 logMAR in the whole sample (p < 0.001). Regarding binocularity, the binocular function (BF) score also experienced a significant improvement (p < 0.001), with a mean change of 1.55 with therapy in the whole sample. The BCVA of the dominant eye only improved significantly (p < 0.001) in the isometropic amblyopic subgroup. In conclusion, the use of the dichoptic therapy with the digital platform evaluated allows an effective restoration of visual acuity and binocular function in children with anisometropic and isometropic amblyopia.
The Role of Psychological Inflexibility and Experiential Approach on Mental Health in Children and Adolescents: An Exploratory Study
Authors: Torres-Fernández Gloria , Rodríguez-Valverde Miguel , Reyes-Martín Salvador , Hernández-Lopez Mónica
Published: June 2022

The prevalence of mental health problems during childhood and adolescence is on the rise. There is a growing interest in the examination of personal variables that may function as risk factors and that may be targeted for effective intervention. This study explores the relationships amongst different aspects of psychological inflexibility (one, typically studied, focusing on the individual’s responding to unwanted emotions and cognitions, and another, more recently explored, focusing on the individual’s responding to desired thoughts and affective states), emotional intelligence, and mental health symptoms. A total of 129 school-going children (mean age: 11.16 years old) completed a battery of instruments comprising the Avoidance and Fusion Questionnaire-Youth (AFQ-Y17), the Experiential Approach Scale (EAS), the Emotional Intelligence Quotient Inventory (EQi-YV), and the Revised Child Anxiety and Depression Scale (RCADS-30). Results showed that both the AFQ-Y17 score and an EAS subscale score (Anxious Clinging) were significant independent predictors of mental health symptoms in general. Emotional intelligence was predictive only for depression, and both the AFQ-Y17 and the Anxious Clinging EAS subscale significantly incremented the predictive power of a hierarchical linear regression model including all three variables. These results underscore the relevance of psychological inflexibility for child/adolescent mental health, and the need to further explore a specific aspect of inflexibility regarding positive emotions and other appetitive private events.
How Human-like Behavior of Service Robot Affects Social Distance: A Mediation Model and Cross-Cultural Comparison
Authors: Li Linyao , Li Yi , Song Bo , Shi Zhaomin , Wang Chongli
Published: June 2022

Previous studies on the human likeness of service robots have focused mainly on their human-like appearance and used psychological constructs to measure the outcomes of human likeness. Unlike previous studies, this study focused on the human-like behavior of the service robot and used a sociological construct, social distance, to measure the outcome of human likeness. We constructed a conceptual model, with perceived competence and warmth as mediators, based on social-identity theory. The hypotheses were tested through online experiments with 219 participants from China and 180 participants from the US. Similar results emerged for Chinese and American participants in that the high (vs. low) human-like behavior of the service robot caused the participants to have stronger perceptions of competence and warmth, both of which contributed to a smaller social distance between humans and service robots. Perceptions of competence and warmth completely mediated the positive effect of the human-like behavior of the service robot on social distance. Furthermore, Chinese participants showed higher anthropomorphism (perceived human-like behavior) and a stronger perception of warmth and smaller social distance. The perception of competence did not differ across cultures. This study provides suggestions for the human-likeness design of service robots to promote natural interaction between humans and service robots and increase human acceptance of service robots.
Characteristics, Progress and Trends of Urban Microclimate Research: A Systematic Literature Review and Bibliometric Analysis
Authors: Zhou Yichen , An Na , Yao Jiawei
Published: June 2022

Climate change has been a hot topic in recent years. However, the urban microclimate is more valuable for research because it directly affects people’s living environments and can be adjusted by technological means to enhance the resilience of cities in the face of climate change and disasters. This paper analyses the literature distribution characteristics, development stages, and research trends of urban microclimate research based on the literature on “urban microclimate” collected in the Web of Science core database since 1990, using CiteSpace and VOSviewer bibliometric software. It is found that the literature distribution of the urban microclimate is characterized by continuous growth, is interdisciplinary, and can be divided into four stages: nascent exploration, model quantification, diversified development and ecological synergy. Based on the knowledge mapping analysis of keyword clustering, annual overlap, and keyword highlighting, it can be predicted that the research on foreign urban land patch development has three hot trends—multi-scale modelling, multi-factor impact, and multi-policy guidance. The study’s findings help recognize the literature distribution characteristics and evolutionary lineage of urban microclimate research and provide suggestions for future urban microclimate research.
Effect of Welding Separation Characteristics on the Cyclic Behavior of Steel Plate Shear Walls
Authors: El-Sisi Alaa A. , Elgiar Mohammed M. , Maaly Hassan M. , Shallan Osman A. , Salim Hani A.
Published: June 2022

Currently, the steel plate shear wall (SPSW) is commonly used in high-rise steel buildings as a lateral load-resisting system due to its several advantages such as its lightweight and high ductility and strength. The SPSW consists of two main parts, i.e., the boundary frame and infill plate, which are connected by welding. The objective of this work is to study the effect of the infill plate weld separation on the seismic behavior of the SPSWs. A numerical method was proposed to have a comprehensive comparison of seismic behaviors of different separation characteristics. The model was validated by using previously published experimental works. Key parameters, such as load-carrying capacity, stiffness, and energy-dissipation capacity, were discussed extensively. The unstiffened SPSW (USPSW) system is more sensitive to the plate–beam separation than the plate–column one, especially the corner plate–beam separation. When plate–column welding separation occurs, the initial stiffness and the energy dissipation capacity are reduced by approximately 21% and 14%, respectively; however, the reductions are 36% and 20.5% in the case of beam welding separation.
Sensitivity Analysis for Pedestrian-Induced Vibration in Footbridges
Authors: Wei Xiaojun , Zhang Jingwei , Zhou Hao , Živanović Stana
Published: June 2022

This paper aims to provide a novel insight into the influence of uncertainties in system- and pedestrian-induced load parameters on the vibration response of footbridges. The study begins with a sensitivity analysis for the vertical vibration response of a representative footbridge to two loading cases: a single pedestrian and a crowd. Two methods are utilized: the Sobol’-based global sensitivity analysis method and the local sensitivity analysis method. Uncertainties in all model parameters (which include bridge and human body dynamics in a walking posture, as well as dynamic force generated by humans) are considered in stochastic response estimation. Parametric analysis is then performed to investigate the influence of the variation of the mean values of the bridge modal mass, damping ratio, and natural frequency on the results of global and local sensitivity analysis. Systematic comparison of the results of global and local sensitivity analysis is performed to identify their similarities and differences. It has been found that the sensitive parameters and their importance ranking strongly depend on bridge modal properties and loading scenarios (i.e., a single pedestrian or a crowd crossing). The damping ratio and natural frequency of the human body are found to be the only two insensitive parameters. Therefore, they could be treated as deterministic parameters in the stochastic estimation of human-induced vibration. Global sensitivity analysis is recommended as a choice for the sensitivity analysis of pedestrian-induced vibration of footbridges as it leads to more reliable results, owing to the advantage of characterizing model sensitivity over the entire input spaces.
Algorithmic Generation of Building Typology for Office Building Design
Authors: Androsics-Zetz Dóra Noémi , Kistelegdi István , Ercsey Zsolt
Published: June 2022

Numerous office building design optimizations are in international research to reduce energy consumption, optimize costs and provide optimal comfort. However, there is a lack of knowledge on the effects of geometry and space organization. This study deals with space organization problems and searches for all possible optimal building space structure configurations in terms of energy and comfort parameters using a mathematical algorithmic method. The methodology is based on the formulation of feasible architectural rules and their translation into an algorithm that can generate 2D floor plans satisfying all boundary conditions. In the framework of an exemplary modeling procedure, a 4-story office building geometry generation was carried out, resulting in 17-floor plan versions and 7 different building geometries. The resulting building shapes were classified by energy-related geometry parameters (envelope surface/useful area) for the future step of the research, where the cases will be compared with the help of building simulations. With the help of the method, it was possible to significantly narrow the search space, but future improvements are needed for faster work for wider applicability.
Analytical Procedure for Timber−Concrete Composite (TCC) System with Mechanical Connectors
Authors: Mirdad Md , Khan Rafid , Chui Ying
Published: June 2022

In the construction of modern multi-storey mass timber structures, a composite floor system commonly specified by structural engineers is the timber–concrete composite (TCC) system, where a mass timber beam or mass timber panel (MTP) is connected to a concrete slab with mechanical connectors. The design of TCC floor systems has not been addressed in timber design standards due to a lack of suitable analytical models for predicting the serviceability and safety performance of these systems. Moreover, the interlayer connection properties have a large influence on the structural performance of a TCC system. These connection properties are often generated by testing. In this paper, an analytical approach for designing a TCC floor system is proposed that incorporates connection models to predict connection properties from basic connection component properties such as embedment and withdrawal strength/stiffness of the connector, thereby circumventing the need to perform connection tests. The analytical approach leads to the calculation of effective bending stiffness, forces in the connectors, and extreme stresses in concrete and timber of the TCC system, and can be used in design to evaluate allowable floor spans under specific design loads and criteria. An extensive parametric analysis was also conducted following the analytical procedure to investigate the TCC connection and system behaviour. It was observed that the screw spacing and timber thickness remain the most important parameters which significantly influence the TCC system behaviour.
Digital Synthesis of Realistically Clustered Carbon Nanotubes
Authors: Susi Bryan T. , Tu Jay F.
Published: June 2022

A computational approach for creating realistically structured carbon nanotubes is presented to enable more accurate and impactful multi-scale modeling and simulation techniques for nanotube research. Much of the published literature to date involving computational modeling of carbon nanotubes simplifies their structure as being long and straight, and often existing as isolated individual nanotubes. However, imagery of nanotubes has shown over several decades that nanotubes agglomerate together and exhibit looping and curvature due both to inter- and intra-nanotube attraction. The research presented in this paper leverages multi-scale simulations consisting of a simple bead-spring model for initial nanotube relaxation followed by a differential geometry approach to create an atomic representation of carbon nanotubes, and then finalized with molecular dynamics simulations using the Tersoff potential model for carbon that allows dynamic bonding and cleavage. The result is atomically accurate representations of carbon nanotubes that exist as single nanotubes, or as clusters of multiple nanotubes. The presented approach is demonstrated using (5,5) single-walled carbon nanotubes. The synthesized nanotubes are shown to relax into the curving and looping structures observed in transmission or scanning electron microscopy, but also exhibit nano-scale defects due to buckling, crimping, and twisting that are resolved during the molecular dynamics simulations. These features locally compromise the desired strength characteristics of nanotubes and therefore the presented procedure will enable more accurate modeling and simulation of nanotubes in subsequent research by representing them less as the theoretically straight and independent entities, but as realistically imperfect.
Considerations for the Use of Machine Learning Extracted Real-World Data to Support Evidence Generation: A Research-Centric Evaluation Framework
Authors: Estevez Melissa , Benedum Corey M. , Jiang Chengsheng , Cohen Aaron B. , Phadke Sharang , Sarkar Somnath , Bozkurt Selen
Published: June 2022

A vast amount of real-world data, such as pathology reports and clinical notes, are captured as unstructured text in electronic health records (EHRs). However, this information is both difficult and costly to extract through human abstraction, especially when scaling to large datasets is needed. Fortunately, Natural Language Processing (NLP) and Machine Learning (ML) techniques provide promising solutions for a variety of information extraction tasks such as identifying a group of patients who have a specific diagnosis, share common characteristics, or show progression of a disease. However, using these ML-extracted data for research still introduces unique challenges in assessing validity and generalizability to different cohorts of interest. In order to enable effective and accurate use of ML-extracted real-world data (RWD) to support research and real-world evidence generation, we propose a research-centric evaluation framework for model developers, ML-extracted data users and other RWD stakeholders. This framework covers the fundamentals of evaluating RWD produced using ML methods to maximize the use of EHR data for research purposes.
GNAQ and GNA11 Genes: A Comprehensive Review on Oncogenesis, Prognosis and Therapeutic Opportunities in Uveal Melanoma
Authors: Silva-Rodríguez Paula , Fernández-Díaz Daniel , Bande Manuel , Pardo María , Loidi Lourdes , Blanco-Teijeiro María José
Published: June 2022

The GNAQ and GNA11 genes are mutated in almost 80–90% of uveal melanomas in a mutually exclusive pattern. These genes encode the alpha subunits of the heterotrimeric G proteins, Gq and G11; thus, mutations of these genes result in the activation of several important signaling pathways, including phospholipase C, and activation of the transcription factor YAP. It is well known that both of them act as driver genes in the oncogenic process and it has been assumed that they do not play a role in the prognosis of these tumours. However, it has been hypothesised that mutations in these genes could give rise to molecularly and clinically distinct types of uveal melanomas. It has also been questioned whether the type and location of mutation in the GNAQ and GNA11 genes may affect the progression of these tumours. All of these questions, except for their implications in carcinogenesis, remain controversial. Uveal melanoma has a distinctive genetic profile, and specific recurrent mutations, which make it a potential candidate for treatment with targeted therapy. Given that the most frequent mutations are those observed in the GNAQ and GNA11 genes, and that both genes are involved in oncogenesis, these molecules, as well as the downstream signalling pathways in which they are involved, have been proposed as promising potential therapeutic targets. Therefore, in this review, special attention is paid to the current data related to the possible prognostic implications of both genes from different perspectives, as well as the therapeutic options targeting them.
Risk Factors and Clinicopathological Features for Developing a Subsequent Primary Cutaneous Squamous and Basal Cell Carcinomas
Authors: Ciążyńska Magdalena , Pabianek Marta , Sławińska Martyna , Reich Adam , Lewandowski Bogumił , Szczepaniak Katarzyna , Ułańska Małgorzata , Nejc Dariusz , Brodowski Robert , Sobjanek Michał , Owczarek Witold , Kamińska-Winciorek Grażyna , Lange Dariusz , Słowińska Monika , Wróbel Katarzyna , Bieniek Andrzej , Woźniacka Anna , Pękala Anika , Kuncman Łukasz , Salińska Magdalena , Noweta Marcin , Skibińska Małgorzata , Narbutt Joanna , Ciążyński Karol , Lewandowska Marta , Dziankowska-Zaborszczyk Elżbieta , Lesiak Aleksandra
Published: June 2022

Background: Patients with diagnosed keratinocyte carcinomas (KCs) have an increased risk of subsequent skin cancers development. Current studies indicate that patients with subsequent tumors should be followed up regularly. However, none of the studies indicate the connection between the specific subtypes and an increased risk for further KCs development. The study assesses the differences in the risk of developing a subsequent skin cancer after a previous diagnosis of KC, especially considering individual types of skin malignances, and identifies potential factors associated with an increased risk of new cutaneous tumor describing non-invasive diagnosis and monitoring. Methods: Pathology and medical records were examined to identify the characteristics of patients with multiple KCs diagnosed between 1999 and 2019. Results: The study group comprised 13,913 KCs occurring in 10,083 patients. Multiple KCs were observed in 2300 patients (22.8%). The analysis showed aggressive subtypes, multiple tumors, and male sex as significant prognostic factors. Conclusions: The most crucial risk factors for developing subsequent KC are being of a male gender, an aggressive tumor subtype, and previous history of multiple skin cancers. Basal cell carcinoma subtypes, such as infiltrative basosquamous, with aggressive growth patterns predispose not only to increased risk for the recurrence but are also expected to be at higher risk of subsequent KCs.
Unveiling the Potential of Novel Struvite–Humic Acid Composite Extracted from Anaerobic Digestate for Adsorption and Reduction of Chromium
Authors: Wang Xiqing , Muhmood Atif , Yu Haizhong , Li Yuqi , Fan Wenying , Tian Pengjiao
Published: June 2022

A novel struvite–humic acid composite (S–HA) was derived from an anaerobic digestate and evaluated for the adsorption and reduction of chromium [Cr (VI)] in this study. The results indicated that the struvite–humic acid composite (S–HA) contains higher contents of oxygen-containing and aromatic functional groups (47.05% and 34.13%, respectively) and a higher specific surface area (19.3 m2 g−1). These special characteristics of S–HA contributed to its higher adsorption capacity (207.69 mg g−1 and 254.47 mg g−1 for pseudo-first and second-order kinetic models, respectively) for chromium. Furthermore, XPS analysis showed that a portion of the bonded Cr (VI) was reduced to Cr (III) by carboxyl and hydroxyl functional groups, which oxidized and changed into ketone and phenol functional groups. Based on the findings, it was concluded that the phosphate–humic acid composite has an outstanding chromium adsorptive and reduction capacity. However, more research is needed to fully understand the potential of the struvite–humic acid composite for chromium adsorption and reduction.
Kinetic Study and Modeling of Wild-Type and Recombinant Broccoli Myrosinase Produced in E. coli and S. cerevisiae as a Function of Substrate Concentration, Temperature, and pH
Authors: Jiménez Adielis , Castillo Antonio , Mahn Andrea
Published: June 2022

The myrosinase enzyme hydrolyzes glucosinolates, among which is glucoraphanin, the precursor of the anticancer isothiocyanate sulforaphane (SFN). The main source of glucoraphanin is Brassicaceae; however, its natural concentration is relatively low, limiting the availability of SFN. An option to obtain SFN is its exogenous production, through enzymatic processes and under controlled conditions, allowing complete conversion of glucoraphanin to SFN. We characterized the kinetics of wild-type (BMYR) and recombinant broccoli myrosinases produced in E. coli (EMYR) and S. cerevisiae (SMYR) in terms of the reaction conditions. Kinetics was adjusted using empirical and mechanistic models that describe reaction rate as a function of substrate concentration, temperature, and pH, resulting in R2 values higher than 90%. EMYR kinetics differed significantly from those of BMYR and SMYR probably due to the absence of glycosylations in the enzyme produced in E. coli. BMYR and SMYR were subjected to substrate inhibition but followed different kinetic mechanisms attributed to different glycosylation patterns. EMYR (inactivation Ea = 76.1 kJ/mol) was more thermolabile than BMYR and SMYR. BMYR showed the highest thermostability (inactivation Ea = 52.8 kJ/mol). BMYR and EMYR showed similar behavior regarding pH, with similar pK1 (3.4 and 3.1, respectively) and pK2 (5.4 and 5.0, respectively), but differed considerably from SMYR.
Pixel Super-Resolution Phase Retrieval for Lensless On-Chip Microscopy via Accelerated Wirtinger Flow
Authors: Gao Yunhui , Yang Feng , Cao Liangcai
Published: June 2022

Empowered by pixel super-resolution (PSR) and phase retrieval techniques, lensless on-chip microscopy opens up new possibilities for high-throughput biomedical imaging. However, the current PSR phase retrieval approaches are time consuming in terms of both the measurement and reconstruction procedures. In this work, we present a novel computational framework for PSR phase retrieval to address these concerns. Specifically, a sparsity-promoting regularizer is introduced to enhance the well posedness of the nonconvex problem under limited measurements, and Nesterov’s momentum is used to accelerate the iterations. The resulting algorithm, termed accelerated Wirtinger flow (AWF), achieves at least an order of magnitude faster rate of convergence and allows a twofold reduction in the measurement number while maintaining competitive reconstruction quality. Furthermore, we provide general guidance for step size selection based on theoretical analyses, facilitating simple implementation without the need for complicated parameter tuning. The proposed AWF algorithm is compatible with most of the existing lensless on-chip microscopes and could help achieve label-free rapid whole slide imaging of dynamic biological activities at subpixel resolution.
Mitochondria and Doxorubicin-Induced Cardiomyopathy: A Complex Interplay
Authors: Schirone Leonardo , D’Ambrosio Luca , Forte Maurizio , Genovese Riccardo , Schiavon Sonia , Spinosa Giulia , Iacovone Giuliano , Valenti Valentina , Frati Giacomo , Sciarretta Sebastiano
Published: June 2022

Cardiotoxicity has emerged as a major side effect of doxorubicin (DOX) treatment, affecting nearly 30% of patients within 5 years after chemotherapy. Heart failure is the first non-cancer cause of death in DOX-treated patients. Although many different molecular mechanisms explaining the cardiac derangements induced by DOX were identified in past decades, the translation to clinical practice has remained elusive to date. This review examines the current understanding of DOX-induced cardiomyopathy (DCM) with a focus on mitochondria, which were increasingly proven to be crucial determinants of DOX-induced cytotoxicity. We discuss DCM pathophysiology and epidemiology and DOX-induced detrimental effects on mitochondrial function, dynamics, biogenesis, and autophagy. Lastly, we review the current perspectives to contrast the development of DCM, which is still a relatively diffused, invalidating, and life-threatening condition for cancer survivors.
Cell–Fibronectin Interactions and Actomyosin Contractility Regulate the Segmentation Clock and Spatio-Temporal Somite Cleft Formation during Chick Embryo Somitogenesis
Authors: Gomes de Almeida Patrícia , Rifes Pedro , Martins-Jesus Ana P. , Pinheiro Gonçalo G. , Andrade Raquel P. , Thorsteinsdóttir Sólveig
Published: June 2022

Fibronectin is essential for somite formation in the vertebrate embryo. Fibronectin matrix assembly starts as cells emerge from the primitive streak and ingress in the unsegmented presomitic mesoderm (PSM). PSM cells undergo cyclic waves of segmentation clock gene expression, followed by Notch-dependent upregulation of meso1 in the rostral PSM which induces somite cleft formation. However, the relevance of the fibronectin matrix for these molecular processes remains unknown. Here, we assessed the role of the PSM fibronectin matrix in the spatio-temporal regulation of chick embryo somitogenesis by perturbing (1) extracellular fibronectin matrix assembly, (2) integrin–fibronectin binding, (3) Rho-associated protein kinase (ROCK) activity and (4) non-muscle myosin II (NM II) function. We found that integrin–fibronectin engagement and NM II activity are required for cell polarization in the nascent somite. All treatments resulted in defective somitic clefts and significantly perturbed meso1 and segmentation clock gene expression in the PSM. Importantly, inhibition of actomyosin-mediated contractility increased the period of hairy1/hes4 oscillations from 90 to 120 min. Together, our work strongly suggests that the fibronectin–integrin–ROCK–NM II axis regulates segmentation clock dynamics and dictates the spatio-temporal localization of somitic clefts.
Layered Double Hydroxide/Nanocarbon Composites as Heterogeneous Catalysts: A Review
Authors: Tichit Didier , Álvarez Mayra G.
Published: June 2022

The synthesis and applications of composites based on layered double hydroxides (LDHs) and nanocarbons have recently seen great development. On the one hand, LDHs are versatile 2D compounds that present a plethora of applications, from medicine to energy conversion, environmental remediation, and heterogeneous catalysis. On the other, nanocarbons present unique physical and chemical properties owing to their low-dimensional structure and sp2 hybridization of carbon atoms, which endows them with excellent charge carrier mobility, outstanding mechanical strength, and high thermal conductivity. Many reviews described the applications of LDH/nanocarbon composites in the areas of energy and photo- and electro-catalysis, but there is still scarce literature on their latest applications as heterogeneous catalysts in chemical synthesis and conversion, which is the object of this review. First, the properties of the LDHs and of the different types of carbon materials involved as building blocks of the composites are summarized. Then, the synthesis methods of the composites are described, emphasizing the parameters allowing their properties to be controlled. This highlights their great adaptability and easier implementation. Afterwards, the application of LDH/carbon composites as catalysts for C–C bond formation, higher alcohol synthesis (HAS), oxidation, and hydrogenation reactions is reported and discussed in depth.
The Impact of Natural Disasters on Maternal Health: Hurricanes Irma and María in Puerto Rico
Authors: Lafarga Previdi Irene , Welton Michael , Díaz Rivera Jazmín , Watkins Deborah J. , Díaz Zulmarie , Torres Héctor R. , Galán Chrystal , Guilloty Natacha I. , Agosto Luis D. , Cordero José F. , Alshawabkeh Akram , Vélez Vega Carmen M.
Published: June 2022

The PROTECT research Center funded by the NIH’s National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program was launched in 2010 to explore the impact of exposure to pollutants on the high rate of premature births in Puerto Rico. In September 2017, Hurricanes Irma and María devastated the archipelago, which caused: collapse of the electrical system, collapse of the communication system, limited access to clean water, food, gas, and health services, destruction of public (e.g., hospitals) and private property (e.g., houses) and more than 4500 deaths. Pregnant and postpartum individuals are especially vulnerable to natural disasters. They face difficulty obtaining adequate pre- and post-natal care, are exposed to increased risk of miscarriage, premature delivery, and giving birth to low birth weight babies during and after disasters and are also more likely to suffer physical and mental health problems compared to the general population during and after disasters. A face-to-face questionnaire was administered to PROTECT participants who were pregnant during hurricanes Irma or Maria or who became pregnant shortly after in order to identify hurricane-related sources of stress and other adverse effects. This paper is based on the answers to the open-ended question at the end of the questionnaire where participants were asked to share their experiences during and after the hurricanes. Among the 375 participants who completed the survey, 76 answers to the open-ended question were considered due to data saturation. The answers to the open-ended question were transcribed into a document in order to facilitate the coding process. The transcribed text was analyzed first to identify emerging categories and then coded to identify common themes as well as divergence among participants. The following themes were identified: pregnancy and birth challenges, lack of access to basic services, housing conditions, stressful working conditions, concerns about health, concerns about their children, and positive or protective aspects. The results indicate how the disruption in access to basic services has a unique impact on the physical and mental health of pregnant and post-partum women in an emergency situation. These findings point to the potential benefit of developing specific protocols designed for emergency preparedness aimed at this population, which can inform healthcare providers and community organizations in case of future events.
Microstructure and Properties in Simulated Seawater of Copper-Doped Micro-arc Coatings on TC4 Alloy
Authors: Zhang Yong , Yang Wei , Yu Sen , Wang Liqun , Ma Xiqun , Gao Wei , Lan Nan , Shao Wenting , Chen Jian
Published: June 2022

Micro-arc oxidation (MAO) ceramic coatings were prepared on TC4 titanium alloys by adding CuSO4 to a (NaPO3)6 base solution. The microstructures of the MAO coatings were characterized by scanning electron microscopy (SEM), energy dispersive (EDS), and X-ray photoelectron spectroscopy (XPS). The corrosion resistance and wear resistance of these coatings were evaluated via hydrochloric acid immersion of weight deficit and friction tests. Those results indicated the presence of Cu in the MAO coating in the form of CuO and Cu2O. Incorporation of CuSO4 results in a thickness and roughness increase in the coating. The coating has a lower coefficient of friction (0.2) upon the addition of 4 g/L of CuSO4. The antibacterial properties of the MAO coatings were maximized at 6 g/L of CuSO4. However, the corrosion resistance of the copper-doped MAO coating did not exceed the undoped coating. This study shows that the addition of CuSO4 to the electrolyte successfully prepared copper-containing micro-arc oxidation coatings, which improved the wear resistance and antibacterial properties of the coating.
Humic Acid Removal in Water via UV Activated Sodium Perborate Process
Authors: Yuan Deling , Zhai Zhihui , Zhu Eryu , Liu Huilin , Jiao Tifeng , Tang Shoufeng
Published: June 2022

Humic acid (HA) has complex molecular structure and is capable of adsorption, ion exchange, and chelation with organic and inorganic pollutants in water bodies, worsening water quality and jeopardizing human health and ecological environment. How to effectively remove HA from water is one of the research focuses of this paper. In this study, the UV-activated sodium perborate (SPB) synergistic system (UV/SPB) was established to eliminate HA in water. The effects of initial HA concentration, SPB dose, and initial pH value on the HA elimination were determined, and the main mechanisms of the synergy and HA degradation were explored. The outcomes show that the HA elimination ratio by the sole UV and only SPB system were only 0.5% and 1.5%, respectively. The HA removal of UV/SPB reached 88.8%, which can remove HA more effectively than other systems. Free radical masking experiment proved that hydroxyl radical produced by SPB activation is the main active substance for HA removal. The results of UV-vis absorption spectrum, absorbance ratio, specific UV absorbance, and excitation–emission matrix spectroscopy verified that the UV/SPB system can effectively decompose and mineralize HA.
Learning-Oriented QoS- and Drop-Aware Task Scheduling for Mixed-Criticality Systems
Authors: Ranjbar Behnaz , Alikhani Hamidreza , Safaei Bardia , Ejlali Alireza , Kumar Akash
Published: June 2022

In Mixed-Criticality (MC) systems, multiple functions with different levels of criticality are integrated into a common platform in order to meet the intended space, cost, and timing requirements in all criticality levels. To guarantee the correct, and on-time execution of higher criticality tasks in emergency modes, various design-time scheduling policies have been recently presented. These techniques are mostly pessimistic, as the occurrence of worst-case scenario at run-time is a rare event. Nevertheless, they lead to an under-utilized system due to frequent drops of Low-Criticality (LC) tasks, and creation of unused slack times due to the quick execution of high-criticality tasks. Accordingly, this paper proposes a novel optimistic scheme, that introduces a learning-based drop-aware task scheduling mechanism, which carefully monitors the alterations in the behaviour of the MC system at run-time, to exploit the generated dynamic slacks for reducing the LC tasks penalty and preventing frequent drops of LC tasks in the future. Based on an extensive set of experiments, our observations have shown that the proposed approach exploits accumulated dynamic slack generated at run-time, by 9.84% more on average compared to existing works, and is able to reduce the deadline miss rate by up to 51.78%, and 33.27% on average, compared to state-of-the-art works.
Structural Degradation of O3-NaMnO2 Positive Electrodes in Sodium-Ion Batteries
Authors: Palluzzi Matteo , Silvestri Laura , Celeste Arcangelo , Tuccillo Mariarosaria , Latini Alessandro , Brutti Sergio
Published: June 2022

In this manuscript, we report an extensive study of the physico-chemical properties of different samples of O3-NaMnO2, synthesized by sol–gel and solid state methods. In order to successfully synthesize the materials by sol–gel methods a rigorous control of the synthesis condition has been optimized. The electrochemical performances of the materials as positive electrodes in aprotic sodium-ion batteries have been demonstrated. The effects of different synthesis methods on both structural and electrochemical features of O3-NaMnO2 have been studied to shed light on the interplay between structure and performance. Noticeably, we obtained a material capable of attaining a reversible capacity exceeding 180 mAhg−1 at 10 mAg−1 with a capacity retention >70% after 20 cycles. The capacity fading mechanism and the structural evolution of O3-NaMnO2 upon cycling have been extensively studied by performing post-mortem analysis using XRD and Raman spectroscopy. Apparently, the loss of reversible capacity upon cycling originates from irreversible structural degradations.
Recent Trends in Elpasolite Single Crystal Scintillators for Radiation Detection
Authors: Jin Taiguang , Hao Shuwei , Shang Yunfei , Lei Zuotao , Yang Chunhui
Published: June 2022

Scintillation detection has attracted great interest in nuclear medicine, nuclear radiation detection, high-energy physics, and non-destructive inspection. The elpasolite crystals with Ce3+ dopants are promising for these endeavors due to their modest light yield and extremely good proportionality when excited by the gamma ray. Moreover, the 6Li and 35Cl isotopes in elpasolite crystals endow them with excellent neutron detection capability. These features allow not only a high energy resolution but also a high detection sensitivity. The elpasolite scintillators also enable the precisely dual detection of gamma/neutron signals through pulse height discrimination (PHD) or pulse shape discrimination (PSD). In this work, we review recent investigations on using the typical elpasolite scintillators, including Ce3+-doped Cs2LiYCl6 (CLYC), Cs2LiLaCl6 (CLLC), and Cs2LiLaBr6 (CLLB), for the monitoring of gamma rays and neutrons. The scintillation properties, detection mechanism, and elpasolite crystal structure are also discussed with the aim of improving high-energy ray detection ability.
Coaxial Drainage versus Standard Chest Tube after Pulmonary Lobectomy: A Randomized Controlled Study
Authors: Bassi Massimiliano , Mottola Emilia , Mantovani Sara , Amore Davide , Pagini Andreina , Diso Daniele , Vannucci Jacopo , Poggi Camilla , De Giacomo Tiziano , Rendina Erino Angelo , Venuta Federico , Anile Marco
Published: June 2022

Chest tubes are routinely inserted after thoracic surgery procedures in different sizes and numbers. The aim of this study is to assess the efficacy of Smart Drain Coaxial drainage compared with two standard chest tubes in patients undergoing thoracotomy for pulmonary lobectomy. Ninety-eight patients (57 males and 41 females, mean age 68.3 ± 7.4 years) with lung cancer undergoing open pulmonary lobectomy were randomized in two groups: 50 received one upper 28-Fr and one lower 32-Fr standard chest tube (ST group) and 48 received one 28-Fr Smart Drain Coaxial tube (SDC group). Hospitalization, quantity of fluid output, air leaks, radiograph findings, pain control and costs were assessed. SDC group showed shorter hospitalization (7.3 vs. 6.1 days, p = 0.02), lower pain in postoperative day-1 (p = 0.02) and a lower use of analgesic drugs (p = 0.04). Pleural effusion drainage was lower in SDC group in the first postoperative day (median 400.0 ± 200.0 mL vs. 450.0 ± 193.8 mL, p = 0.04) and as a mean of first three PODs (median 325.0 ± 137.5 mL vs. 362.5 ± 96.7 mL, p = 0.01). No difference in terms of fluid retention, residual pleural space, subcutaneous emphysema and complications after chest tubes removal was found. In conclusion, Smart Drain Coaxial chest tube seems a feasible option after thoracotomy for pulmonary lobectomy. The SDC group showed a shorter hospitalization and decreased analgesic drugs use and, thus, a reduction of costs.