Deuterium Labels to Study Biodegradation of Plastics with Raman Spectroscopy&#160;
Authors: Müller Kara , Elsner Martin , Ivleva Natalia P. 
Published: March 2024

Biodegradable polymers are considered one of the solutions to the plastic accumulation problem in terrestrial and aquatic systems. It is important to ensure complete degradation since residual micro- and nanoplastics influence soil health and its biota. During biodegradation, microorganisms first colonize the plastic surface, where they then excrete enzymes responsible for the depolymerization. Finally, the mono- and oligomers are utilized by the microorganisms as energy sources (mineralization into CO2) or for biomass formation. Only by studying the last step the final fate of the anthropogenic pollutant is revealed. Conventionally CO2 concentrations are measured to monitor microbial activity in samples exposed to plastics in comparison to plastic-free controls. However, this is in no direct relation to the polymer and priming effects or unknown processes due to bacteria adaptation might blur the analysis. Stable isotope labels can be traced from the polymer into 13CO2, D2O and microbial biomass to overcome those obstacles. While many publications covered 13CO2 monitoring, only Zumstein et al. additionally traced the carbon label into fungal biomass with nanoscale secondary ion mass spectrometry.[1] In our approach, we use deuterium instead of carbon labels due to reduced costs and enhanced availability of labeled compounds. Although we lose the ability to contribute to a closed mass balance, we use non-destructive Raman microspectroscopy to gain additional chemical information on a single cell level. Heavier isotopes lead to a red shift of the according Raman band due to their larger mass. Deuteration of microbial lipids, proteins, DNA, and carbohydrates leads to an extensive shift of C-H vibrations into the Raman-silent region. C-D vibrations can therefore be quickly detected with a facilitated data analysis.We incubated the environmental bacterium Sphingomonas koreensis with deuterated polylactic acid (dPLA) in an aqueous medium at room temperature under aerobic conditions. After 3 weeks, we observed an additional biomass spectrum for about 50 % of the measured cells besides undeuterated biomass and dPLA particles. After 13 weeks, this spectrum was already recorded for all cells. While the biomass and C&#8209;H str. vibrations clearly indicate microbial biomass, the C-D vibrations of the additional spectra differ from reference deuterated biomass spectra obtained with glucose-d12 and D2O labeling. After comparing these untypical C-D vibrations to self-obtained and literature reference spectra, they were interpreted to originate from deuterated biomass with strongly deuterated lipids and inhibited labeling of proteins. Now that we can trace deuterium from labeled plastics into microbial biomass, we want to extend the approach to terrestrial environments. Therefore, cell isolation from the soil matrix was successfully adapted from the literature[2] to gain adequate Raman spectra. In ongoing experiments, environmental samples will first be exposed to unlabeled PLA for bacteria adaptation and then used for incubation with dPLA in soil microcosms.&#160;References:Zumstein, M.T., et al., Science Advances, 2018. 4(7): p. eaas9024.
Eichorst, S.A., et al., FEMS Microbiology Ecology, 2015. 91(10).
Acknowledgments: Deutsche Forschungsgemeinschaft (DFG) Project IV 110/2-2 and International Atomic Energy Agency (IAEA) for funding different parts of the research. Dr. J&#252;rgen Allgaier (FZ J&#252;lich) for providing the deuterated PLA.