bluuebunny/crossref_metadata_2025 huggingface dataset:
Peptidomic Analysis of Neonate Umbilical Cord Blood for the Identification of Endogenous Peptides Involved in Hypoxic–Ischemic Encephalopathy
Authors: Dong Xiaohua , Zhao Jing , Shen Yinyin , Sun Qing , Wu Xiaohui , Zhu Yanqing , Yu Lingling , Zhao Yingmin
Published: August 2021

Neonatal hypoxic–ischemic encephalopathy (HIE) is a common neurological disorder triggered by perinatal cerebral ischemia and hypoxia. Accumulating evidence has shown that peptides have neuroprotective effects in nerve injury. However, the function of endogenous peptides in the pathogenesis of HIE has not been studied. In the present study, a comparative peptidomic profile was performed in the serum of the human umbilical cord blood with HIE (three patients) and the control group (three health control) by liquid chromatography–mass spectrometry (LC-MS). Our study demonstrated that a total of 49 peptides derived from 25 precursor proteins were differentially expressed in the serum of HIE compared with normal controls, including 33 upregulated peptides and 16 downregulated peptides. Each of the differentially expressed peptides has specific characteristics, including pI, Mw, and cleavage pattern. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the precursor proteins of differentially expressed peptides participate in the different biological process. Moreover, among the 49 differentially expressed peptides, 21 peptides were identified from the fibrinogen chain family, which plays a role in neurological diseases, suggesting that these peptides may play an important role in maintaining brain health. In conclusion, our results showed a comparative peptidomic profile from human umbilical cord blood of HIE patients and normal controls. These dysregulated peptides may have potentially important functions in umbilical cord blood with HIE and may be involved in the pathogenesis of the HIE.
An Automated Approach for General Movement Assessment: A Pilot Study
Authors: Fontana Camilla , Ottaviani Valeria , Veneroni Chiara , Sforza Sofia E. , Pesenti Nicola , Mosca Fabio , Picciolini Odoardo , Fumagalli Monica , Dellacà Raffaele L.
Published: August 2021

Objective: The objective of the study was to develop an automatic quantitative approach to identify infants with abnormal movements of the limbs at term equivalent age (TEA) compared with general movement assessment (GMA).

Methods: GMA was performed at TEA by a trained operator in neonates with neurological risk. GMs were classified as normal (N) or abnormal (Ab), which included poor repertoire and cramped synchronized movements. The signals from four micro-accelerometers placed on all limbs were recorded for 10 min simultaneously. A global index (KC_index), quantifying the characteristics of individual limb movements and the coordination among the limbs, was obtained by adding normalized kurtosis of the distribution of the first principal component of the acceleration signals to the cross-correlation of the jerk for the upper and lower limbs.

Results: Sixty-eight infants were studied. A KC_index cut-off of 201.5 (95% CI: 199.9–205.0) provided specificity = 0.86 and sensitivity = 0.88 in identifying infants with Ab movements.

Conclusions: KC_index provides an automatic and quantitative measure that may allow the identification of infants who require further neurological evaluation.
African and Asian Medicinal Plants as a Repository for Prospective Antiviral Metabolites Against HIV-1 and SARS CoV-2: A Mini Review
Authors: Anywar Godwin , Akram Muhammad , Chishti Muhammad Amjad
Published: August 2021

Introduction:The worldwide burden of viral infections has triggered a resurgence in the search for new and more efficient antiviral drugs. Scientists are also repurposing existing natural compounds such as the antimalarial drug artemisinin fromArtemesia annuaL. as potential drug candidates for some of the emerging and re-emerging viral infections such as covid-19

Aim:The aim of this review was to analyse the existing literature to explore the actual or potential natural antiviral compounds from African and Asian medicinal plants as lead compounds in the drug discovery process.

Methods:We searched the literature on African and Asian medicinal plant species as antiviral agents for HIV-1 and the novel coronavirus (SARS-CoV-2) in various databases and search engines such as Web of Science, Google Scholar and PubMed. The search was limited toin vitro,in vivo, and clinical studies and excludedin silicostudies.

Results:We present 16 plant species with actual or potential antiviral activity against HIV-1 and SARS-CoV-2. These plant species span the continents of Africa and Asia where they are widely used for treating several other ailments.

Conclusion:Natural compounds from plants can play a significant role in the clinical management of HIV/AIDS and the covid-19 pandemic. More research needs to be conducted to investigate the potential toxicities of the various compounds and their efficacies in clinical settings.
Baicalein Potentiated M1 Macrophage Polarization in Cancer Through Targeting PI3Kγ/ NF-κB Signaling
Authors: He Shan , Wang Shangshang , Liu Suqing , Li Zheng , Liu Xiao , Wu Jinfeng
Published: August 2021

Baicalein is one of the bioactive compounds extracted from Scutellaria baicalensis. Recent studies indicated the antitumor effects of baicalein, however, the underlying mechanisms are needed to be further determined. In this study, we found that baicalein could inhibit the tumor growth in mice models of breast cancer and melanoma and worked as an immunomodulator to promote the infiltration of tumor-associated macrophages (TAMs) and skew the TAMs towards the M1-like phenotype. Baicalein also induced M1-like phenotype polarization in THP-1-derived macrophages. Meanwhile, the expression of pro-inflammatory factors associated with M1 macrophages, including TNF-α, IL-1β, CXCL9 and CXCL10, were increased after baicalein treatment. Mechanistically, the RNA-seq data suggested that baicalein potentiated the M1 macrophage polarization via the NF-κB/TNF-α signaling pathway. ELISA and confocal microscopy assay confirmed that baicalein significantly induced the production of TNF-α and the activation of NF-κB, while TNF-α neutralization inhibited baicalein-induced macrophage polarization toward M1, and NF-κB P65 knock-down suppressed baicalein-induced TNF-α production in THP-1-derived macrophages. Phosphoinositide 3-kinase (PI3k) γ has been reported as a key molecule in macrophage polarization, and inhibition of PI3Kγ activates the NF-κB-related inflammatory signals. Our pharmacological network analysis predicted that PI3Kγ might be one of the major targets of baicalein. The results from the docking program and surface plasmon resonance (SPR) confirmed that baicalein displayed good binding activity to PI3Kγ. We further found that baicalein not only exhibited a direct inhibitory effect on the protein kinase activity of PI3Kγ, but also reduced the mRNA and protein expression of PI3Kγ, indicating that baicalein might be a novel PI3Kγ inhibitor. In summary, baicalein mediated the TAMs skewing to M1-TAMs, and then retarded tumor growth. These effects, at least in part, were linked to the PI3Kγ/NF-κB signaling.
Detection of Brief Episodes of Atrial Fibrillation Based on Electrocardiomatrix and Convolutional Neural Network
Authors: Salinas-Martínez Ricardo , de Bie Johannes , Marzocchi Nicoletta , Sandberg Frida
Published: August 2021

Background:Brief episodes of atrial fibrillation (AF) may evolve into longer AF episodes increasing the chances of thrombus formation, stroke, and death. Classical methods for AF detection investigate rhythm irregularity or P-wave absence in the ECG, while deep learning approaches profit from the availability of annotated ECG databases to learn discriminatory features linked to different diagnosis. However, some deep learning approaches do not provide analysis of the features used for classification. This paper introduces a convolutional neural network (CNN) approach for automatic detection of brief AF episodes based on electrocardiomatrix-images (ECM-images) aiming to link deep learning to features with clinical meaning.

Materials and Methods:The CNN is trained using two databases: the Long-Term Atrial Fibrillation and the MIT-BIH Normal Sinus Rhythm, and tested on three databases: the MIT-BIH Atrial Fibrillation, the MIT-BIH Arrhythmia, and the Monzino-AF. Detection of AF is done using a sliding window of 10 beats plus 3 s. Performance is quantified using both standard classification metrics and the EC57 standard for arrhythmia detection. Layer-wise relevance propagation analysis was applied to link the decisions made by the CNN to clinical characteristics in the ECG.

Results:For all three testing databases, episode sensitivity was greater than 80.22, 89.66, and 97.45% for AF episodes shorter than 15, 30 s, and for all episodes, respectively.

Conclusions:Rhythm and morphological characteristics of the electrocardiogram can be learned by a CNN from ECM-images for the detection of brief episodes of AF.
Stimulation of the Epithelial Na+ Channel in Renal Principal Cells by Gs-Coupled Designer Receptors Exclusively Activated by Designer Drugs
Authors: Soares Antonio G. , Contreras Jorge , Archer Crystal R. , Mironova Elena , Berdeaux Rebecca , Stockand James D. , Abd El-Aziz Tarek Mohamed
Published: August 2021

The activity of the Epithelial Na+ Channel (ENaC) in renal principal cells (PC) fine-tunes sodium excretion and consequently, affects blood pressure. The Gs-adenylyl cyclase-cAMP signal transduction pathway is believed to play a central role in the normal control of ENaC activity in PCs. The current study quantifies the importance of this signaling pathway to the regulation of ENaC activity in vivo using a knock-in mouse that has conditional expression of Gs-DREADD (designer receptors exclusively activated by designer drugs; GsD) in renal PCs. The GsD mouse also contains a cAMP response element-luciferase reporter transgene for non-invasive bioluminescence monitoring of cAMP signaling. Clozapine N-oxide (CNO) was used to selectively and temporally stimulate GsD. Treatment with CNO significantly increased luciferase bioluminescence in the kidneys of PC-specific GsD but not control mice. CNO also significantly increased the activity of ENaC in principal cells in PC-specific GsD mice compared to untreated knock-in mice and CNO treated littermate controls. The cell permeable cAMP analog, 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate, significantly increased the activity and expression in the plasma membrane of recombinant ENaC expressed in CHO and COS-7 cells, respectively. Treatment of PC-specific GsD mice with CNO rapidly and significantly decreased urinary Na+ excretion compared to untreated PC-specific GsD mice and treated littermate controls. This decrease in Na+ excretion in response to CNO in PC-specific GsD mice was similar in magnitude and timing as that induced by the selective vasopressin receptor 2 agonist, desmopressin, in wild type mice. These findings demonstrate for the first time that targeted activation of Gs signaling exclusively in PCs is sufficient to increase ENaC activity and decrease dependent urinary Na+ excretion in live animals.
Predictive Chromatography of Leaf Extracts Through Encoded Environmental Forcing on Phytochemical Synthesis
Authors: Bacong Junelle Rey C. , Juanico Drandreb Earl O.
Published: August 2021

Environment fluctuations can influence a plant's phytochemical profile via phenotypic plasticity. This adaptive response ensures a plant's survival under fluctuating growth conditions. However, the resulting plant extract composition becomes unpredictable, which is a problem for highly standardized medicinal applications. Here we demonstrate, for the first time, the feasibility of tracking the changes in the phytochemical profile based on real-time measurements of a few environment and extract-preparation variables. As a result, we predicted the chromatograms of Blumea balsamifera extracts through an imputation-augmented convolutional neural network, which uses the image-transformed temporal measurements of the variables. We developed a sensor network that collected data in a greenhouse and a training algorithm that concurrently generated a data representation of the implicit plant-environment interactions leading to the mutable chromatograms of leaf extracts. We anticipate the generic applicability of the method for any plant and recognize its potential for addressing the standardization problems in plant therapeutics.
Phenotypic Diversity and Plasticity of Photoresponse Across an Environmentally Contrasting Family of Phytoflagellates
Authors: Clegg Mark R. , Wacker Alexander , Spijkerman Elly
Published: August 2021

Organisms often employ ecophysiological strategies to exploit environmental conditions and ensure bio-energetic success. However, the many complexities involved in the differential expression and flexibility of these strategies are rarely fully understood. Therefore, for the first time, using a three-part cross-disciplinary laboratory experimental analysis, we investigated the diversity and plasticity of photoresponsive traits employed by one family of environmentally contrasting, ecologically important phytoflagellates. The results demonstrated an extensive inter-species phenotypic diversity of behavioural, physiological, and compositional photoresponse across the Chlamydomonadaceae, and a multifaceted intra-species phenotypic plasticity, involving a broad range of beneficial photoacclimation strategies, often attributable to environmental predisposition and phylogenetic differentiation. Deceptively diverse and sophisticated strong (population and individual cell) behavioural photoresponses were observed, with divergence from a general preference for low light (and flexibility) dictated by intra-familial differences in typical habitat (salinity and trophy) and phylogeny. Notably, contrasting lower, narrow, and flexible compared with higher, broad, and stable preferences were observed in freshwater vs. brackish and marine species. Complex diversity and plasticity in physiological and compositional photoresponses were also discovered. Metabolic characteristics (such as growth rates, respiratory costs and photosynthetic capacity, efficiency, compensation and saturation points) varied elaborately with species, typical habitat (often varying more in eutrophic species, such as Chlamydomonas reinhardtii), and culture irradiance (adjusting to optimise energy acquisition and suggesting some propensity for low light). Considerable variations in intracellular pigment and biochemical composition were also recorded. Photosynthetic and accessory pigments (such as chlorophyll a, xanthophyll-cycle components, chlorophyll a:b and chlorophyll a:carotenoid ratios, fatty acid content and saturation ratios) varied with phylogeny and typical habitat (to attune photosystem ratios in different trophic conditions and to optimise shade adaptation, photoprotection, and thylakoid architecture, particularly in freshwater environments), and changed with irradiance (as reaction and harvesting centres adjusted to modulate absorption and quantum yield). The complex, concomitant nature of the results also advocated an integrative approach in future investigations. Overall, these nuanced, diverse, and flexible photoresponsive traits will greatly contribute to the functional ecology of these organisms, addressing environmental heterogeneity and potentially shaping individual fitness, spatial and temporal distribution, prevalence, and ecosystem dynamics.
Embryogenic Calli Induction and Salt Stress Response Revealed by RNA-Seq in Diploid Wild Species Gossypium sturtianum and Gossypium raimondii
Authors: Nie Hushuai , Wang Yali , Wei Chengcheng , Grover Corrinne E. , Su Ying , Wendel Jonathan F. , Hua Jinping
Published: August 2021

Wild cotton species can contribute to a valuable gene pool for genetic improvement, such as genes related to salt tolerance. However, reproductive isolation of different species poses an obstacle to produce hybrids through conventional breeding. Protoplast fusion technology for somatic cell hybridization provides an opportunity for genetic manipulation and targeting of agronomic traits. Transcriptome sequencing analysis of callus under salt stress is conducive to study salt tolerance genes. In this study, calli were induced to provide materials for extracting protoplasts and also for screening salt tolerance genes. Calli were successfully induced from leaves of Gossypium sturtianum (C1 genome) and hypocotyls of G. raimondii (D5 genome), and embryogenic calli of G. sturtianum and G. raimondii were induced on a differentiation medium with different concentrations of 2, 4-D, KT, and IBA, respectively. In addition, embryogenic calli were also induced successfully from G. raimondii through suspension cultivation. Transcriptome sequencing analysis was performed on the calli of G. raimondii and G. sturtianum, which were treated with 200 mM NaCl at 0, 6, 12, 24, and 48 h, and a total of 12,524 genes were detected with different expression patterns under salt stress. Functional analysis showed that 3,482 genes, which were differentially expressed in calli of G. raimondii and G. sturtianum, were associated with biological processes of nucleic acid binding, plant hormone (such as ABA) biosynthesis, and signal transduction. We demonstrated that DEGs or TFs which related to ABA metabolism were involved in the response to salt stress, including xanthoxin dehydrogenase genes (ABA2), sucrose non-fermenting 1-related protein kinases (SnRK2), NAM, ATAT1/2, and CUC2 transcription factors (NAC), and WRKY class of zinc-finger proteins (WRKY). This research has successfully induced calli from two diploid cotton species and revealed new genes responding to salt stress in callus tissue, which will lay the foundation for protoplast fusion for further understanding of salt stress responses in cotton.
CEO Turnover, Leadership Identity, and TMT Creativity in a Cross-Cultural Context
Authors: Rong Pengfei , Wang Chao
Published: August 2021

Whether chief executive officer (CEO) turnover can improve top management team (TMT) creativity is an important issue that remains to be solved. Based on the theoretical background of CEO turnover, team creativity, and cross-cultural context, this study proposes a theoretical model to answer the question and introduces leadership identity as a moderator simultaneously. The multiple regression analysis of data obtained from 903 executives in 104 top management teams revealed CEO voluntary resignation/internal succession pattern, CEO voluntary resignation/external succession pattern, and CEO forced resignation/internal succession pattern separately had a significant positive impact on TMT creativity in a cross-cultural context; leadership identity partially moderated the relationship between CEO turnover and TMT creativity. According to these findings, only three patterns of CEO turnover could promote TMT creativity, and leadership identity enhanced the positive effects of CEO voluntary resignation/internal succession pattern, CEO voluntary resignation/external succession pattern, and CEO forced resignation/internal succession pattern on TMT creativity in a cross-cultural context. These made up for the lack of theoretical research on the relationships among CEO turnover, TMT creativity and leadership identity, which could provide the scientific guidance to conduct the CEO turnover practice and improve TMT creativity in a cross-cultural context.
Entrepreneurship, Corporate Social Responsibilities, and Innovation Impact on Banks’ Financial Performance
Authors: Wei Jianhua , Xiong Rong , Hassan Marria , Shoukry Alaa Mohamd , Aldeek Fares Fawzi , Khader J. A.
Published: August 2021

The basic aim of this research was to check the impact of innovation, corporate social responsibilities (CSR), and entrepreneurship on the monetary performance of banks in five different countries: Qatar, Pakistan, China, the United States (US), and France. This research was conducted to measure the relationship of these factors and innovative workforce activities. The secondary data were collected from websites of twenty five banks in different countries, including Islamic and conventional banks. Different econometric analyses, such as descriptive statistical analysis, correlation coefficient test for measuring the interaction, and ordinary least square regression analysis for determining the impact of dependent and independent variables, were carried out. In the present study, entrepreneurship, CSR, and innovation were taken as independent variables. Board size, frequency of assemblies, and self-employed with large shareholders were included as sub-parts of entrepreneurship. On the other hand, the financial performance of banks was taken as the dependent variable. Return on assets (ROA) and return on equity (ROE) were considered parts of economic performance. The overall conclusions drawn in this study showed that there was a significant relationship between all the studied variables. The research provided useful insights into the long-debated question regarding the relevance of entrepreneurship and CSR.
Regulating Self-Image on Instagram: Links Between Social Anxiety, Instagram Contingent Self-Worth, and Content Control Behaviors
Authors: Lopez Richard B. , Polletta Isabel
Published: August 2021

Social media platforms have provided human beings with unprecedented ways to virtually connect with one another, creating a novel and complex arena for psychological research. Indeed, a growing body of research has uncovered links between social media use and various aspects of health and wellbeing. However, relatively little work has examined factors that characterize how people experience and regulate their online selves on particular platforms. In the present study, we recruited a large sample of active Instagram users (N= 247; ages 18–58) to complete a questionnaire battery that included measures of participants' social anxiety, their sense of self-worth tied to Instagram use, and specific content control behaviors on the Instagram platform (e.g., editing captions, disabling comments, etc.). Results indicated that participants with higher levels of social anxiety tended to have greater Instagram contingent self-worth, and this was then associated with some content control behaviors, including editing captions and photos and videos when sharing posts. These findings suggest that those who are more socially anxious interact with Instagram differently, and this may arise from self-worth that is wedded to their experiences on the platform. Overall, this work adds to a growing body of research highlighting the benefits and risks of social media use on psychological health.
The Influence of E-Learning and Emotional Intelligence on Psychological Intentions: Study of Stranded Pakistani Students
Authors: Alam Fahad , Yang Qing , Bhutto Muhammad Yaseen , Akhtar Nadeem
Published: August 2021

The COVID-19 pandemic has forced the government to close the educational institutions globally, to contain the infection of the disease, which has affected the academic activities of local and international students significantly. This unexpected shift from offline classes to online learning has created psychological disruption among the students. At that backdrop, this study aims to investigate the influence of e-learning and emotional intelligence (EI) on the study stress, burnout, and performance of Pakistani students by applying emotion regulation theory. The data (N = 387) is based on international students, enrolled in 10 different universities in China. The results indicate that both e-learning and EI have significantly affected perceived study stress, burnout, and performance of students. These findings have provided evidence that online classes and EI can influence study stress, burnout, and performance of students. The study concludes that EI has significant impact on the psychological pressure of a student.
The Association Between Sexism, Self-Sexualization, and the Evaluation of Sexy Photos on Instagram
Authors: Plieger Thomas , Groote Olivia , Hensky Rachel , Hurtenbach Lea , Sahler Sharon , Thönes Luise , Reuter Martin
Published: August 2021

Passive consumption of sexually objectifying content on social networking sites (SNS) has been shown to result in lowered body satisfaction and self-esteem, particularly in women. However, deliberate evaluations of sexually objectifying social media content are scarce. Furthermore, associations between self-objectification and active use of SNS in terms of posting behavior have not been shown so far. The present study asked N = 916 participants to rate 28 Instagram screenshots on three dimensions, namely, whether the given photos were perceived as sexually revealing, appropriate, and attractive. The ratings were related to sexist attitudes, enjoyment of sexualization, and posting behavior of the participants. Sexism was negatively associated with the perceived appropriateness of the presented Instagram photos in women. Furthermore, there were substantial correlations between appropriateness and attractiveness evaluations of the presented photos and the self-sexualizing posting behavior and enjoyment of sexualization of female users. Only inconsistent effects could be found in men. Greater appreciation of objectification seems to go along with self-objectifying behavior, which may be due to an altered perception of what can be considered as sexually revealing. Although effects seem to be smaller in men, future research should also focus on male enjoyment of sexualization, which unfortunately was not considered in the present study.
Qatar Healthcare Workers' COVID-19 Vaccine Hesitancy and Attitudes: A National Cross-Sectional Survey
Authors: Kumar Rajeev , Alabdulla Majid , Elhassan Nahid M. , Reagu Shuja Mohd
Published: August 2021

Introduction: Healthcare workers are the critical frontline workforce of the COVD-19 pandemic and are considered a target group for vaccination. Hesitancy to vaccinate is a major concern that can jeopardize the vaccination programme. The hesitancy rates in the general population and healthcare workers (HCWs) vary globally, and more importantly, hesitancy in HCWs is of particular concern, as it can influence the wider population.

Materials and Methods: The present study evaluated the vaccine hesitancy rate and its sociodemographic and attitudinal factors among the HCWs in the state of Qatar. We conducted a national cross-sectional survey using a validated hesitancy measurement tool between October 15 and November 15, 2020. A total of 7,821 adults above the age of 18 years out of the 2.3 million adult Qatari residents completed the survey. While majority of the participants were from the general public, 1,546 participants were HCWs. Sociodemographic data, along with attitudes and beliefs around COVID-19 vaccination, were collected from the respondents.

Results: We found that 12.9% of the study participants showed vaccine hesitancy, defined as definitely or probably will not take the vaccine if offered, and 25.31% reported that they were unsure about the uptake of the COVID-19 vaccine. Female respondents were more hesitant toward the vaccine. Safety and efficacy concerns of vaccine were the significant predictors of vaccine hesitancy. The primary predictor for vaccine acceptance was a better understanding of the disease and vaccine.

Discussion: Overall, 1 in 8 HCWs were reluctant to get vaccinated against COVID-19, mainly due to concerns about the vaccine's efficacy and safety. Education about the vaccine's safety and efficacy can potentially improve acceptance among healthcare workers.
Workplace Relations and Opportunities for Career Development Impact the Retention of Veterinarians in Shelter Medicine
Authors: Powell Lauren , Reinhard Chelsea L. , Serpell James , Watson Brittany
Published: August 2021

Shelter medicine has grown considerably over recent years with many shelters hiring veterinarians for the first time or expanding their veterinary teams. As a result, there is a dearth of shelter veterinarians and retention has become a key concern for the field. The goal of this study was to describe veterinarians' perceptions of shelter medicine, and their feelings of job satisfaction, loneliness, and professional fulfillment. The sample included 52 shelter veterinarians, 39 previous shelter veterinarians and 130 non-shelter veterinarians (n = 221) who each completed an online survey. Current and previous shelter veterinarians had comparable perceptions regarding the appeal of most shelter medicine duties, although there were differences in the duties they performed within their job. More current shelter veterinarians participated in population management, policy development, administrative duties, and decision-making for individual patients (euthanasia, treatment, and adoptability). Considering other employment attributes, we found previous shelter veterinarians had lower mean rankings than current and non-shelter veterinarians regarding their interactions with administrative staff, ability to be part of a multiple veterinarian team and the availability of mentorship. Loneliness and professional fulfillment were mostly comparable between the groups, although previous shelter veterinarians were more likely to report they felt unhappy (X2 = 16.60, p = 0.02) and left out at work (X2 = 12.43, p = 0.02). Our findings suggest veterinarians who participate in decision-making for patients and shelter management procedures may be more willing to continue working in shelter medicine. Animal shelters should also employ strategies to improve workplace relationships and offer career development opportunities to improve job satisfaction and retention of veterinarians within the field.
Transmission Comparison for Cooperative Robotic Applications
Authors: Nandor Mark J. , Heebner Maryellen , Quinn Roger , Triolo Ronald J. , Makowski Nathaniel S.
Published: August 2021

The development of powered assistive devices that integrate exoskeletal motors and muscle activation for gait restoration benefits from actuators with low backdrive torque. Such an approach enables motors to assist as needed while maximizing the joint torque muscles, contributing to movement, and facilitating ballistic motions instead of overcoming passive dynamics. Two electromechanical actuators were developed to determine the effect of two candidate transmission implementations for an exoskeletal joint. To differentiate the transmission effects, the devices utilized the same motor and similar gearing. One actuator included a commercially available harmonic drive transmission while the other incorporated a custom designed two-stage planetary transmission. Passive resistance and mechanical efficiency were determined based on isometric torque and passive resistance. The planetary-based actuator outperformed the harmonic-based actuator in all tests and would be more suitable for hybrid exoskeletons.
Signal Grass Deferred Pastures Fertilized with Nitrogen or Intercropped with Calopo
Authors: Chaves Carla Silva , Ribeiro Karina Guimarães , Pereira Odilon Gomes , da Fonseca Dilermando Miranda , Cecon Paulo Roberto , Gomide Carlos Augusto de Miranda
Published: August 2021

This study aimed to evaluate the accumulation, structural characteristics, and chemical composition of deferred signal-grass pastures that were subjected to four treatments: without nitrogen fertilization, intercropped with calopo (Calopogonium mucunoides), and fertilized with urea N (50 kg ha−1 and 100 kg ha−1) for 2 years. The design was in randomized blocks, with two blocks and two repetitions of each treatment per block. There were effects of the interaction between treatment and year on green dry mass, forage accumulation, density of vegetative tillers, and crude protein content (simulated grazing). The effects of the treatments on the height, falling index, green dry mass/dead dry mass ratio, number of dead, live and total tillers, and crude protein content (direct cutting) were also observed. Signal-grass–calopo-intercropping ensured adequate mass and forage accumulation and crude protein content equivalent to those of fertilized pastures. In addition, the intercropped pasture showed a higher percentage of leaves and a higher crude protein content compared with those for the other treatments (simulated grazing). The green dry mass/dead dry mass ratio was highest in the intercropped pasture and was equivalent to only that of the pasture fertilized with a low dose of nitrogen. Therefore, signal-grass–calopo-intercropping may be recommended for deferment.
Yield Components Stability Assessment of Peas in Conventional and Low-Input Cultivation Systems
Authors: Greveniotis Vasileios , Bouloumpasi Elisavet , Zotis Stylianos , Korkovelos Athanasios , Ipsilandis Constantinos G.
Published: August 2021

The primary purpose of this study was to explore yield stability of pea (Pisum sativum L.) cultivars based on stability index, with specific aim at studying cultivar behavior regarding yield of peas under both conventional and low-input cultivation systems. Five cultivars of peas were used in a strip-plot design. Correlations showed a significant positive relation between seed yield and some other traits. Indirect seed yield improvement may be implemented by improving pod length, which generally showed high stability indices in Greek mega-environment. Comparisons between conventional and low-input farming systems generally did not affect stability estimations, but revealed cultivars that exhibited stable performance, even in low-input farming systems. The additive main effects and multiplicative interaction (AMMI) biplot analysis, genotype by environment interaction (GGE) biplot analysis and analysis of variance (ANOVA) showed statistically significant differences between genotypes and environments, and also the farming system. This way, we have certain cultivars of peas to recommend for specific areas and farming system, in order to achieve the most stable performance. Vermio proved to be a stable cultivar for seed yield performance, in Giannitsa, Trikala and Kalambaka area, in low-inputs farming systems, while Olympos was the best in Florina area and low-input farming.
Impact of Nitrogen Rate in Conventional and Organic Production Systems on Yield and Bread Baking Quality of Soft Red Winter Wheat
Authors: Al-Zubade Ammar , Phillips Timothy , Williams Mark A. , Jacobsen Krista , Van Sanford David
Published: August 2021

Soft red winter wheat (SRW) is characterized by high yield and relatively low protein content. In Kentucky, there is growing demand from local artisan bread bakers for regionally produced flour, requiring production of grain with increased protein content and/or strength. The objective of this two-year field experiment was to evaluate the effect of nitrogen (N) management on five cultivars of winter wheat on yield and bread baking quality traits of modern and landrace SRW cultivars (Triticum aestivum L.). All five cultivars were evaluated using two N application rates in conventional and organic production systems. All traits measured were significantly affected by the agricultural production system and N rate, although plant height and other quality traits varied by study year. Significantly higher yields were achieved in the conventional system at a relatively low N rate (67.2 kg ha−1) in both study years (2017–2019) (p < 0.01). Results were variable by cultivar and a locally bred, high-yielding cultivar (Pembroke 2014) had the highest lactic acid solvent retention capacity score and thousand kernel weight of the cultivars evaluated. In addition, a landrace cultivar (Purple Straw) had the highest grain N and plant height. A French soft wheat, Soissons, had the highest sedimentation value and Pembroke 2016 achieved the highest yield. The findings from this study suggest the possibility of attaining a desirable grain with quality traits of SRW wheat that meets the needs of local bread wheat production in Kentucky through improving the optimization of cultivar selection, N management and specific considerations for conventional and organic systems.
Modeling Natural Light Availability in Skyscraper Farms
Authors: Eaton Michael , Harbick Kale , Shelford Timothy , Mattson Neil
Published: August 2021

Lighting is a major component of energy consumption in controlled environment agriculture (CEA) operations. Skyscraper farms (multilevel production in buildings with transparent glazing) have been proposed as alternatives to greenhouse or plant factories (opaque warehouses) to increase space-use efficiency while accessing some natural light. However, there are no previous models on natural light availability and distribution in skyscraper farms. This study employed climate-based daylight modeling software and the Typical Meteorological Year (TMY) dataset to investigate the effects of building geometry and context shading on the availability and spatial distribution of natural light in skyscraper farms in Los Angeles (LA) and New York City (NYC). Electric energy consumption for supplemental lighting in 20-storey skyscraper farms to reach a daily light integral target was calculated using simulation results. Natural lighting in our baseline skyscraper farms without surrounding buildings provides 13% and 15% of the light required to meet a target of 17 mol·m−2·day−1. More elongated buildings may meet up to 27% of the lighting requirements with natural light. However, shading from surrounding buildings can reduce available natural light considerably; in the worst case, natural light only supplies 5% of the lighting requirements. Overall, skyscraper farms require between 4 to 11 times more input for lighting than greenhouses per crop canopy area in the same location. We conclude that the accessibility of natural light in skyscraper farms in dense urban settings provides little advantage over plant factories.
Effects of Growing Cycle and Genotype on the Morphometric Properties and Glucosinolates Amount and Profile of Sprouts, Microgreens and Baby Leaves of Broccoli (Brassica oleracea L. var. italica Plenck) and Kale (B. oleracea L. var. acephala DC.)
Authors: Di Bella Maria Concetta , Toscano Stefania , Arena Donata , Moreno Diego A. , Romano Daniela , Branca Ferdinando
Published: August 2021

Some new foods (sprouts, microgreens and baby leaf) of the brassica genus are appreciated for their nutritional and nutraceutical values. The aim of this experimental trial was to improve the nutraceutical traits of these foods by evaluating the effects of the climatic condition, genotype, and plant growth stage on the development of greater quality in relation to these new foods. The morphometric and glucosinolates (GLSs) traits of three traditional Italian cultivars of Brassica oleracea crops, such as broccoli (B. oleracea var. italica), namely the traditional Sicilian landrace ‘Broccolo Nero’ (BN) and the commercial ‘Cavolo Broccolo Ramoso Calabrese’ (CR), as well as the commercial kale cultivar ‘Cavolo Laciniato Nero di Toscana’ (CL) (B. oleracea var. acephala DC.), were evaluated in an unheated greenhouse in Catania during the end of 2019 and the beginning of 2020. Plant growth was studied at different phenological stages—from seeds to sprouts, microgreens, and baby leaves—over two growing cycles, one in autumn–winter and the other in spring–summer. ‘Broccolo Nero’ (BN) broccoli showed more rapid growth and biomass production than the other two cultivars evaluated (i.e., weight, hypocotyl length, and leaf width). The GLS profile varied significantly (p < 0.05), in relation both to plant’s growth stage and to genotype. The highest amount of glucoraphanin was detected for BN microgreens and baby leaves, about 8 µmol g−1 d.w., whereas glucobrassicin and its related derivatives were about 14 µmol g−1 d.w. in microgreens and baby leaves of CL and about 15 µmol g−1 d.w. and 10 µmol g−1 d.w. for glucoraphanin in CR, respectively. These new foods can also be produced at home with simple and cheap equipment
Thermodynamic Concept of Water Retention and Physical Quality of the Soil
Authors: Smagin Andrey V.
Published: August 2021

The physical quality of the soil is determined by its interfacial interactions in conditions of variable water content. In this regard, water retention characteristics in the form of functions of water content and its thermodynamic potentials are used as indicators of physical quality and its dynamics in the soil. The combination of centrifugation and thermodesorption methods allowed for the first time the assessment of soil water potentials in the entire range of variation from 0 to 106 J/kg for a representative database (more than 400 samples) of the main genetic types of Eurasian soils, grouped into 5 FAO/USDA soil texture classes. The main fundamental achievement of the research is a physically based diagnosis of the critical values of water content and its thermodynamic potentials that separate the areas of dominance of various forms of soil water, physical forces, and mechanisms of interfacial interactions on the WRC-diagrams of the physical quality of the soil. Theoretical and experimental results of the study are of practical interest of sustainable agronomy for determining the optimal ranges of water content in the soil during plant cultivation, water saving, and salt protection in irrigation, mechanical tillage, and other technological operations.
Modelling the Organic Evolution of a Mediterranean Limestone Soil under Usual Cropping of Durum Wheat and Faba Bean
Authors: Ibrahim Hatem , Gaieb Sinda , Brahim Nadhem , Blavet Didier , Van den Meersche Karel , Pansu Marc
Published: August 2021

The modeling of carbon (C) and nitrogen (N) fluxes between microorganisms and plants in pure and associated cultures of durum wheat and faba bean demonstrated a close link between the C and N cycles in agroecosystems. The MOMOS (microorganisms and organic matter of soils) model integrates simplified descriptions of photosynthesis (origin of organic C in soil), N microbial exchange (soil origin for N), N fixation (atmospheric origin for N), and plant growth with an organic matter decomposition core that has the soil microbial community at its center. This work provides estimates of the exchange parameters between plant organs and microbes, which were compared to literature data when available. In a connection with photosynthesized C, the root demand for inorganic N can be adjusted by its microbial production. Our approach is a new methodology for improving plant production, by optimizing the interactions with soil microorganisms. Additionally, the coupling of plant growth and microbial processes enabled determining changes of the organic compartments of soil. In the unfertilized limestone soil of this study, sequestration was found to be located in the labile microbial metabolites for one year, then significantly transferred to stable humus during 6-year intercropping. Thus, we propose the MOMOS mathematical tool, not only for guiding ecological intensification, but also related to the management of agroecosystems for climate change mitigation.
Adaptation of Grain Legumes to Terminal Drought after Rice Harvest in Timor-Leste
Authors: Gusmao Marcal , Freitas Angelo da Costa , Peregrina Avelina M. , Siddique Kadambot H. M.
Published: August 2021

In Timor-Leste, most paddy fields are abandoned after rice harvest due to limited water resources for another rice production cycle, particularly in lowland coastal areas. There is substantial scope for including legumes and other crops in the rice–fallow system in Timor-Leste. This study investigated the adaptation of grain legumes to terminal drought. The experiment was undertaken in 2018 and 2019 at field sites in Vemase and Laleia, respectively, on the northeast coast of Timor-Leste. The experiments used a split-plot design with two factors (water treatment and species) and three blocks (Vemase site) or four blocks (Laleia site). In 2018, the water treatments were well-watered control (W0), water withheld from flower initiation to maturity (W1), and water withheld after seedling establishment to maturity (W2). In 2019, the water treatments were well-watered control (W0) and water withheld from flower initiation to maturity (W1). Grain legumes were mungbean and soybean tested against grass pea (cv. Ceora), a well-known drought-adapted grain legume. The measured parameters included soil water content, crop phenology, plant growth and development, yield and yield components. The experiments revealed that mungbean is the most suitable grain legume crop after rice harvest under moderate drought conditions, while soybean is the preferred option under severe drought. Grass pea could be the best adaptive grain legume under severe drought in Timor-Leste when combined with the worsening conditions of climate change.
Improved Oral Health and Adaptation to Treatment in Dogs Using Manual or Ultrasonic Toothbrush or Textile of Nylon or Microfiber for Active Dental Home Care
Authors: Olsén Lena , Brissman Anna , Wiman Sara , Eriksson Fanny , Kaj Camilla , Brunius Enlund Karolina
Published: August 2021

Poor oral health is a common problem in dogs causing pain and impaired welfare. The gold standard to maintain good oral health and prevent periodontitis is daily tooth brushing. However, many dog owners find it difficult, and dogs often show aversive behavior. There are several different tools available, and in this intra-subject study, four different active dental home care treatment options were examined: manual and ultrasonic toothbrush, a nylon glove, and a microfiber finger cloth. The treatments were evaluated for efficacy against gingivitis, plaque, and calculus in beagle dogs. Each dog received two treatments on the buccal sides of the teeth once daily, either two types of toothbrushes (N = 10) or two types of textiles (N = 11), on separate sides of the mouth. The dogs’ teeth were assessed before and after the five-week treatment period, with the assessor blinded by additional, similar dogs (N = 7). The study also assessed the dogs’ stress levels during treatment using a fear, anxiety, stress (FAS) protocol. All four treatments improved dog oral health, reducing gingivitis and plaque. Furthermore, the amount of calculus also decreased in some individuals. Adaptation to dental care occurred in the dogs over time. This information can be used to encourage dog owners to brush their dogs’ teeth.
Anti-Wrinkle Effect of Isatis indigotica Leaf Extract: Evaluation of Antioxidant, Anti-Inflammation, and Clinical Activity
Authors: Kim Jin Hyeok , Gao Dan , Jeong Won Seok , Kim Cheong Taek , Cho Chong Woon , Kim Hyung Min , Kang Jong Seong
Published: August 2021

Isatis indigotica leaf is an oriental herbal medicine that has been known for various pharmacological effects. However, its anti-wrinkle activity has not been fully evaluated. Therefore, we evaluated the anti-wrinkle effect of I. indigotica leaf extract on human skin. The purified extract inhibited 85.4% of 2,2-diphenyl-1-1picrylhydrazyl and 72.2% of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radicals at a concentration of 1 mg/mL. Nitrite production was reduced by 30% after treatment with 50 μg/mL of extract. Three fractions from the extract downregulated the mRNA expression of matrix metalloproteinase-1 and -3 and upregulated the expression of interleukin 4. Among the three fractions, fraction 2 exhibited the highest activity. The major component of the extract was identified as 3,4,5-trimethoxycinnamic acid by liquid chromatography coupled with mass spectrometry. Molecular docking was conducted to predict the binding mechanism of 3,4,5-trimethoxycinnamic with matrix metalloproteinase-1 and -3, and their binding energies were −5.20 and −4.89 kcal/mol, respectively. In a clinical trial, five roughness values of visiometer and visual score were significantly reduced in treated groups compared with the placebo group after 8 weeks. I. indigotica leaf extract inhibits wrinkle formation, and could be a potential anti-wrinkle agent. This is the first clinical trial demonstrating its anti-wrinkle activity.