bluuebunny/crossref_metadata_2025 huggingface dataset:
Formation mechanism and governance strategies of stigma in public health emergencies: Based on event system theory
Authors: Liu Shuzhen , Liu Yuanyuan , Guo Ming , Wang Rui , Sun Qiong , Zhu Rong
Published: January 2023

Introduction

With the new coronavirus (COVID-19) pandemic across the world, it is critical to propose effective strategies for stigma governance in public health emergencies in order to reduce negative effects caused by stigma. However, no known research has focused on the essential role of events in understanding stigma phenomenon from the perspective of external dynamic changes.

Methods

Based on the event system theory, this paper analyzes the evolution mode and characteristics of specific events in the process of stigmatization from strength, space and time aspects, and taking COVID-19 event as an example, 1202 questionnaires and empirical analysis were conducted.

Results and discussion

Our results reveal that event strength directly affects the results of stigmatization, and such impact appears to be more prominent with a novel, disruptive and critical event. In addition, spatial and temporal attributes represent the dynamic development of an event, and they can interact with event strength to regulate the relationship between event strength and outcomes. Finally, stigma governance strategies under public health emergencies from three aspects of event strength, space, and time were put forward.
Evaluation of the implementation effect of hepatitis C medical insurance reimbursement policy in China: A RWS based on medical institutions
Authors: Liu Yiyao , Gou Liangwen , Guo Zhaoting , Wu Zhiang , He Qin , Feng Haihuan , Hu Ming
Published: January 2023

Objectives

To evaluate the implementation effect of hepatitis C medical insurance reimbursement policy in China from the view of medical institutions.

Methods

The electronic medical record of a top tertiary hospital in Chengdu from January 2014 to December 2020 were extracted, and the interrupted time series model was used to analyze the changes in diagnosis and treatment behavior and disease burden of hepatitis C patients after the implementation of HCV insurance reimbursement policy.

Results

In terms of diagnosis and treatment, the number of visits (β2 = 19.290, P < 0.001) and treatments (β2 = 14.291, P < 0.01) increased instantaneously after the implementation of the outpatient reimbursement policy in Chengdu in 2018, and there was no significant change after the implementation of the single line payment policy for oral direct antiviral (DAA) drugs in 2019 (P > 0.05); in terms of medical expenses, the total treatment cost (β2 = 21439.3, P < 0.001), out-of-pocket expenses (β2 = 6109.44, P < 0.001) and drug expenses (β2 = 21889.8, P < 0.001) of hepatitis C patients have been significantly reduced after the implementation of the single-line payment policy.

Conclusion

Hepatitis C medical insurance reimbursement policy can promote hepatitis C patients to actively seek medical treatment, promote the widespread use of DAA scheme, reduce the burden of patients, and improve the treatment efficiency of hepatitis C.
Older adults' needs and requirements for a comprehensive exergame-based telerehabilitation system: A focus group study
Authors: Seinsche Julia , de Bruin Eling D. , Carpinella Ilaria , Ferrarin Maurizio , Moza Sotiria , Rizzo Francesco , Salatino Claudia , Giannouli Eleftheria
Published: January 2023

Introduction

Telerehabilitation in older adults using information and communication technologies (ICTs) provides therapy, which is potentially equally effective as traditional rehabilitation, yet more accessible. This study aimed to analyze the needs and requirements of older adults (OA) and healthcare-professionals (HP) toward ICTs and telerehabilitation in general as well as toward a specific novel exergame-based telerehabilitation system (COCARE system, Dividat).

Materials and methods

The COCARE telerehabilitation system enables individual training based on exergames, as well as an assessment system and a digital centralized case management. Six focus groups with in total 34 participants were conducted. A mixed-methods approach was used comprising questionnaires and semi-structured interviews.

Results

Both OA and HP would engage to an exergame-based telerehabilitation program. Major motivating factors are the relevance of such a training for health and the entertainment component of exergames. Main requirements are simplification of the system, variety, a personalized training, a constantly available contact person, and comprehensive instructions for use. Besides, HP praised the system's motivational effect, but remained concerned about risk of falls and social isolation.

Conclusion

ICTs for telerehabilitation are accepted by OA and HP but should be adapted hardware- and software-wise to address OA' age-stemming vulnerabilities (e.g., risk of falls) and low ICT literacy.
Prevalence of hepatitis B, C, and D virus infection in Haiti: A systematic review and meta-analysis
Authors: Vincent Jeanne Perpétue , Nyamasege Carolyn , Wang Su , Madec Yoann , Shimakawa Yusuke
Published: January 2023

Background

Viral hepatitis causes an important global health burden. In 2016, the World Health Assembly adopted an objective to globally eliminate this as a public health threat by 2030. However, significant gaps exist between countries in their progress. Haiti is the last country that has introduced infant hepatitis B vaccines into the routine immunization program in the Region of the Americas, and its schedule still does not incorporate birth dose vaccines. As the first step to raise awareness of viral hepatitis in this country, we conducted a systematic review and meta-analysis to estimate the prevalence of hepatitis B (HBV), C (HCV), and D (HDV) viruses in Haiti.

Methods

We searched PubMed, EMBASE, Web of Science and Scopus for studies reporting the prevalence of HBV, HCV and HDV among Haitian, with no language restriction, published until November 30th, 2021. Prevalence was pooled via a random-effects meta-analysis using a generalized linear mixed model with the logit link.

Results

Of 453 articles retrieved, 25 studies were included: 16 reported the prevalence of hepatitis B surface antigen (HBsAg), three for anti-HCV antibody, and six for both HBsAg and anti-HCV. No study was found for HDV prevalence. The pooled prevalence of HBsAg was 0.7% [95% confidence interval (CI): 0.3–1.4, I2 = 77.7%] among children, 3.5% (95% CI: 2.8–4.4, I2 = 93.2%) in the general adult population and 7.4% (95% CI: 4.0–13.3, I2 = 83.9%) in high-risk adult population. The pooled prevalence of anti-HCV antibody was 0.9% (95% CI: 0.6–1.4, I2 = 93.5%) among the general population and 1.4% (95% CI: 0.4–4.2, I2 = 0.0%) in high-risk adult population. No study reported the prevalence of anti-HCV antibody exclusively in children.

Interpretation

The prevalence of blood-borne hepatitis, particularly that of HBV, is substantial in Haiti. The introduction of birth dose hepatitis B vaccines and improving access to testing and treatment services should be urgently considered to meet the elimination goal.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022298081, identifier: PROSPERO (CRD42022298081).
Evolution of beverage portion sizes consumed in Brazil between 2008 and 2018
Authors: Cavalcante Jessica Brito , Vasconcelos Thais Meirelles de , Sichieri Rosely , Bezerra Ilana Nogueira
Published: January 2023

Objective

To describe the evolution of beverage portion sizes consumed in Brazil between 2008 and 2018.

Methods

Data from the dietary surveys of 2008–2009 and 2017–2018 Brazilian Household Budget Surveys, conducted with 34,003 and 46,164 individuals, respectively, were used to analyze the portion size of beverages. Food consumption data were used to group beverages according to nutritional characteristics, type and size of portions into eight groups: high-calorie soft drinks, fruit refreshments, alcoholic beverages, coffee/tea, fruit juices, milk and milk substitutes and ultra-processed milk-based beverages. The two-day food record and recall were considered to analyze the consumed portions. Comparisons between the surveys were done using Chi-Square tests and linear regression models.

Results

Between 2008 and 2018, the average portion consumed showed a significant increase for the group of alcoholic beverages (+29%), flavored juices (+11%), caloric soft drinks (+8%), milk and milk substitutes (+6%) and fruit juices (+5%); and reduction for the coffee/tea group (−11%). Analyzes by age group showed that among individuals between 20 and 40 years of age, the soft drinks and alcoholic beverage groups showed the greatest increase in portion size, +12 and +44%, respectively.

Conclusion

The beverage portion sizes consumed in Brazil between 2008 and 2018 increased for the group of alcoholic beverages, flavored juices, caloric soft drinks, milk and milk substitutes, and fruit juices.
Understanding The Odds: Statistics in Public Health
Authors: Goodwin Gabriella , Ryu So Young
Published: January 2023

In the world of public health and medicine, researchers are often trying to discover new ways of understanding and preventing diseases and other negative health outcomes. When public health researchers want to examine the relationship between some sort of exposure, like smoking, and a disease, such as lung cancer, they will often start by calculating what is called an odds ratio. An odds ratio is a comparison of odds between people who were exposed and people who were not exposed. However, odds ratios can be tricky to understand, even for experienced researchers. In this article, we will break down the odds ratio by reviewing the concepts and calculations of probability and odds. We will also discuss how to interpret an odds ratio, and how these ratios can be useful in real-world applications.
“They attack the family and order”: Right-wing media about feminists and the political consequences of the women's strike in Poland
Authors: Żuk Piotr , Pacześniak Anna
Published: January 2023

The goals of this article are to describe and analyze the main themes in the narratives about feminists that can be found in the populist right-wing press in Poland. Although feminists—alongside LGBT circles, refugees and the left—had been treated as a threat to the existing order before, it was the women's revolt on Polish streets in late autumn 2020 that triggered a series of reports on the women's movement in the right-wing press. In addition to analyzing the content of right-wing weeklies on women's protests, the article also shows how these protests later influenced changes in the political attitudes of young people, social activism and the attitudes of opposition parties in Poland toward cultural and moral issues. The analysis is based on the content analysis of articles published in right-wing weeklies and survey data. The authors conclude that in contemporary Poland the women's movement can contribute to overthrowing not only the rule of the populist right but also the entire conservative order resulting from the post-1989 neoliberal transformation.
Mars’ ionosphere: The key for systematic exploration of the red planet
Authors: Sánchez-Cano Beatriz
Published: January 2023

The near three decades of continuous Mars’ exploration has opened the door to the understanding of the Martian space environment, which includes the solar wind, magnetosphere, ionosphere and atmosphere, and is a complex structure with simultaneous downward and upward couplings. However, we do not yet understand many of the physical processes that drive matter and energy flow between these couplings and within the various atmospheric reservoirs (including temporal and spatial changes on short time scales). Although each coupling plays an essential role for the system, understanding the fate of the ionosphere, as a natural sink of both internal (i.e., atmospheric cycles) and external (i.e., solar wind) energy inputs, is the key for a successful future systematic exploration of Mars.
Spatio-temporal comparisons of the hydrogen-alpha line width and ALMA 3 mm brightness temperature in the weak solar network
Authors: Tarr Lucas A. , Kobelski Adam R. , Jaeggli Sarah A. , Molnar Momchil , Cauzzi Gianna , Reardon Kevin P.
Published: January 2023

Comparisons between the Atacama Large Millimeter/sub-millimeter Array (ALMA) 3 mm emission and a range of optical and UV solar observations have found the strongest correspondence between the width of the hydrogen alpha line at 656.3 nm and the 3 mm brightness temperature. Previous studies on the oscillatory power of p-modes using ALMA Band 3 and Band 6 data in the 3–5 min period bandpass have found a confusing mix of results, with many reporting a complete lack of the p-mode enhancement typically found in other chromospheric observables. We study these issues using an extensive, publicly available coordinated data set targeting a region of weak network flux near disk center at time SOL 2017-03-17T15:42-16:45. We focus on the Interferometric Bidimensional Spectropolarimeter (IBIS) H-alpha and ALMA 3 mm data series. We confirm the strong correlation between the H-alpha line width and the 3 mm brightness temperature, but find a bimodal relation between the two diagnostics, with a shallower slope of 7.4e-5 Å/K in cooler regions and steeper slope of 1.2e-4 Å/K in hotter regions. The origin of the bimodal distribution is unknown, but does hold for the duration of the observations. Both slopes are steeper than a previously reported value, but this is likely due to systematic differences in the analysis. We then calculate the oscillatory power in the H-alpha and 3 mm data. The IBIS data clearly show the p-mode oscillations in spatially averaged power spectra while the ALMA data do not. However, when we remove IBIS data at times corresponding to the ALMA calibration windows, the spatially averaged power spectra for the two data series are nearly identical, with a Pearson correlation coefficient of 0.9895. Further, the power in the two bands remains strongly correlated when the spatial information is retained but the power is integrated over different temporal frequency bands. We therefore argue that the lack of observed p-modes in the ALMA data may be predominantly due to spectral windowing induced by the timing and duration of the calibration observations. Finally, we find that spatial maps of oscillatory power at 3 mm display the pattern of magnetic shadows and halos typically displayed by other chromospheric diagnostics.
Continuous cryotherapy vs. traditional cryotherapy after total knee arthroplasty: A systematic review and meta-analysis of randomized controlled trials
Authors: Liu Meng-Meng , Tian Mian , Luo Changqi , Wang Shicheng , Shao Long
Published: January 2023

Background

Cryotherapy is widely applied to relieve pain and improve functional outcomes after total knee arthroplasty (TKA). New cryotherapy devices have recently been developed to guarantee a fixed temperature for a prolonged time. Therefore, we conducted a systematic review and meta-analysis to compare continuous cryotherapy and traditional cryotherapy (ice bag or gel pack) for patients after TKA.

Methods

This study was conducted according to a predefined protocol registered on PROSPERO. Two independent reviewers performed an electronic database search of PubMed, Embase, Cochrane, Web of Science, Google Scholar, and ClinicalTrials.gov. Dichotomous outcomes were reported as risk difference (RD) with 95% confidence intervals (CIs), and continuous outcomes were reported as mean difference (MD), or standardized mean difference (SMD) with 95% CIs.

Results

Seven trials enrolling a total of 519 patients were included. There were no differences in pain intensity (MD: −0.54, 95% CI: −1.55 to 0.47; P = 0.30), analgesics consumption (MD: −0.37, 95% CI: −1.28 to 0.55; P = 0.43), postoperative range of motion (MD: 0.47, 95% CI: −4.09 to 5.03; P = 0.84), swelling of the knee joint, blood loss, change in hemoglobin, or transfusion rate. Meanwhile, there were no differences in length of hospital stay (MD: −0.77, 95% CI: −1.62 to 0.08; P = 0.07) and adverse events (RD: 0, 95% CI: −0.02 to 0.03; P = 0.74). In addition, continuous cryotherapy leads to extra costs and resources than traditional cryotherapy.

Conclusions

Continuous cryotherapy does not appear to offer significant benefits for TKA when compared with traditional cryotherapy. Based on currently available evidence, traditional cryotherapy is still recommended as continuous cryotherapy is not cost-effective. Further well-designed studies with larger sample sizes are warranted to further confirm these preliminary results.

PROSPERO Registration: Identifier [CRD42022308217].
Posterior unilateral approach with 270° spinal canal decompression and three-column reconstruction using double titanium mesh cage for thoracic and lumbar burst fractures
Authors: Shi Lei , Ge Qi-jun , Cheng Yun , Lin Lu , Yu Qing-Shuai , Cheng Si , Chen Xiao-Lin , Shen Hong-Quan , Chen Fu , Yan Zheng-jian , Wang Yang , Chu Lei , Ke Zhen-Yong
Published: January 2023

Objective

To evaluate the clinical effects of the posterior unilateral approach with 270° spinal canal decompression and three-column reconstruction using double titanium mesh cage (TMC) for thoracic and lumbar burst fractures.

Materials and methods

From May 2013 to May 2018, 27 patients with single-level thoracic and lumbar burst fractures were enrolled. Every patient was followed for at least 18 months. Demographic data, neurologic status, back pain, canal compromise, anterior body compression, operative time, estimated blood loss and surgical-related complications were evaluated. Radiographs were reviewed to assess deformity correction, anterior body height correction, bony fusion and TMC subsidence.

Results

The average preoperative percentages of canal compromise and anterior body height compression were 58.4% and 50.5%, respectively. All surgeries were successfully completed in one phase, the operative time was 151.5 ± 25.5 min (range: 115–220 min), the estimated blood loss was 590.7 ± 169.9 ml (range: 400–1,000 ml). Neurological function recovery was significantly improved except for 3 grade A patients. The preoperative visual analog scale (VAS) scores for back pain were significantly decreased compared with the values at the last follow-up (P = 0.000). The correct deformity angle was 12.4 ± 4.7° (range: 3.9–23.3°), and the anterior body height recovery was 96.7%. The TMC subsidence at the last follow-up was 1.3 ± 0.7 mm (range: 0.3–3.1 mm). Bony fusion was achieved in all patients.

Conclusion

The posterior unilateral approach with 270° spinal canal decompression and three-column reconstruction using double TMC is a clinically feasible, safe and alternative treatment for thoracic and lumbar burst fractures.
A low-carbon high inulin diet improves intestinal mucosal barrier function and immunity against infectious diseases in goats
Authors: Yuan Chunmei , Wang Shuiping , Gebeyew Kefyalew , Yang Xin , Tang Shaoxun , Zhou Chuanshe , Khan Nazir Ahmad , Tan Zhiliang , Liu Yong
Published: January 2023

Introduction

Abrupt weaning is a major stressful event, contributing to intestinal abnormalities and immune system dysfunction in weaned kids. Inulin is a prebiotic fiber with many positive functions, including promoting intestinal fermentation and enhancing host immunity in monogastric animals. However, the effects of a high-inulin, energy-rich diet on ruminal fermentation characteristics, methane emission, growth performance, and immune systems of weaned kids have not been investigated.

Methods

A fully automated in vitro fermentation system was used to investigate ruminal fermentation characteristics and methane emission of a mixed substrate of inulin and fat powder (1.31: 1) in comparison with maize grain-based starter concentrate. During a 1-week adaptation and 4-week trial phase, 18 weaned kids (8.97 ± 0.19 kg) were randomly assigned to two groups, one with a conventional diet (83% maize grain; CON) and the other with a low-carbon, high-inulin diet (41.5% maize grain, 14.4% fat powder, 18.9% inulin; INU).

Results

In the in vitro rumen fermentation experiment, the total gas production was not different (p > 0.05); however, a lower (p < 0.05) methane production was observed for INU as compared to CON. The average daily gain and the ratio of feed intake and growth performance of kids fed with INU were higher (p < 0.05) than those fed with CON. Serum concentrations of alanine transaminase (ALT) and lactate dehydrogenase (LDH) were lower (p < 0.05), whereas the concentration of high-density lipoprotein (HDL) and cholesterol (CHOL) were higher (p < 0.05) in kids fed with the INU diet as compared CON. Dietary inulin significantly increased (p < 0.05) the secretion of immunoglobulins (IgA, IgG, and IgM) and inflammatory cytokines (IFN-γ and IL-10) in ileum tissue. Although no differences (p > 0.05) were observed in mRNA expression of tight junction markers, the INU diet tended to increase (p = 0.09) gene expression of ribosomal protein S6 kinase beta-1 (P70S6K) in the mammalian target of rapamycin (mTOR) pathway of longissimus dorsi muscle.

Conclusion

Our findings highlighted that a low-carbon high-inulin energy-rich diet could be used as a promising strategy to improve gut immunity and growth performance of weaned kids under abrupt weaning stress and reduce methane production.
Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics
Authors: Li Aoyun , Wang Meng , Zhang Yu , Lin Zhengrong , Xu Mengen , Wang Lei , Kulyar Muhammad Fakhar-e-Alam , Li Jiakui
Published: January 2023

Probiotics have attracted attention due to their multiple health benefits to the host. Yaks inhabiting the Tibetan plateau exhibit excellent disease resistance and tolerance, which may be associated with their inner probiotics. Currently, research on probiotics mainly focuses on their positive effects on the host, but information regarding their genome remains unclear. To reveal the potential functional genes of Bacillus subtilis isolated from yaks, we sequenced its whole genome. Results indicated that the genomic length of Bacillus subtilis was 866,044,638 bp, with 4,429 coding genes. The genome of this bacteria was composed of one chromosome and one plasmid with lengths of 4,214,774 and 54,527 bp, respectively. Moreover, Bacillus subtilis contained 86 tRNAs, 27 rRNAs (9 16S_rRNA, 9 23S_rRNA, and 9 5S_rRNA), and 114 other ncRNA. KEGG annotation indicated that most genes in Bacillus subtilis were associated with biosynthesis of amino acids, carbon metabolism, purine metabolism, pyrimidine metabolism, and ABC transporters. GO annotation demonstrated that most genes in Bacillus subtilis were related to nucleic acid binding transcription factor activity, transporter activity, antioxidant activity, and biological adhesion. EggNOG uncovered that most genes in Bacillus subtilis were related to energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism. CAZy annotation found glycoside hydrolases (33.65%), glycosyl transferases (22.11%), polysaccharide lyases (3.84%), carbohydrate esterases (14.42%), auxiliary activities (3.36%), and carbohydrate-binding modules (22.59%). In conclusion, this study investigated the genome and genetic properties of Bacillus subtilis derived from yaks, which contributed to understanding the potential prebiotic mechanism of probiotics from the genetic perspective.
Epigenetic biomarkers for animal welfare monitoring
Authors: Whelan Rose , Tönges Sina , Böhl Florian , Lyko Frank
Published: January 2023

Biomarkers for holistic animal welfare monitoring represent a considerable unmet need in veterinary medicine. Epigenetic modifications, like DNA methylation, provide important information about cellular states and environments, which makes them highly attractive for biomarker development. Up until now, much of the corresponding research has been focused on human cancers. However, the increasing availability of animal genomes and epigenomes has greatly improved our capacity for epigenetic biomarker development. In this review, we provide an overview about animal DNA methylation patterns and the technologies that enable the analysis of these patterns. We also describe the key frameworks for compound DNA methylation biomarkers, DNA methylation clocks and environment-specific DNA methylation signatures, that allow complex, context-dependent readouts about animal health and disease. Finally, we provide practical examples for how these biomarkers could be applied for health and environmental exposure monitoring, two key aspects of animal welfare assessments. Taken together, our article provides an overview about the molecular and biological foundations for the development of epigenetic biomarkers in veterinary science and their application potential in animal welfare monitoring.
Electromechanical Coupling Dynamic and Vibration Control of Robotic Grinding System for Thin-Walled Workpiece
Authors: Liu Yufei , Tang Dong , Ju Jinyong
Published: January 2023

The robotic grinding system for a thin-walled workpiece is a multi-dimensional coupling system composed of a robot, a grinding spindle and the thin-walled workpiece. In the grinding process, a dynamic coupling effect is generated, while the thin-walled workpiece stimulates elastic vibration; the grinding spindle, as an electromechanical coupling actuator, is sensitive to the elastic vibration in the form of load fluctuations. It is necessary to investigate the electromechanical coupling dynamic characteristics under the vibration coupling of the thin-walled workpiece as well as the vibration control of the robotic grinding system. Firstly, considering the dynamic coupling effect between the grinding spindle and thin-walled workpiece, a dynamic model of the grinding spindle and thin-walled workpiece coupling system is established. Secondly, based on this established coupling dynamic model, the vibration characteristics of the thin-walled workpiece and the electromechanical coupling dynamic characteristics of the grinding spindle are investigated. Finally, a speed adaptive control system for the grinding spindle is designed based on a fuzzy PI controller, which can achieve a stable speed for the grinding spindle under vibration coupling and has a certain suppression effect on the elastic vibration of the thin-walled workpiece at the same time.
Modeling Conflicts at Work: The Case of Elementary School Employees in Croatia
Authors: Kostelić Katarina , Paulišić Morena , Gonan Božac Marli
Published: January 2023

This paper aims to explore conflict as a process that includes antecedents, management strategy, and outcomes while examining the role of affective states in an elementary school working environment. After a theoretical overview and systematization of the conflict elements, a questionnaire was developed that satisfies internal reliability and construct validity. Conflicts at the workplace were modeled with Bayesian SEM, and the results point out the prominent role of affective states in conflicts. Findings confirmed that (1) affective states affect the conflict management strategy choice; (2) different conflict management strategies lead to different conflict consequences; and (3) different affective states lead to differently perceived conflict consequences/outcomes. In addition, we conclude that conflicts with dominantly positive and negative affective states behave structurally differently. The revealed role of affective states facilitates future intervention possibilities in education about conflict management strategies and control of emotions and affective states. Raising awareness about affective states’ role in the conflict might increase mutual un-understanding and contribute to a healthy school social environment as a safe space for learning, sharing ideas, and joint actions toward a common goal.
Promoting the New Superior Variety of National Hybrid Maize: Improve Farmer Satisfaction to Enhance Production
Authors: Bahtiar  , Arsyad Muhammad , Salman Darmawan , Azrai Muhammad , Tenrirawe Andi , Yasin Muhammad , Gaffar Abdul , Sebayang Amelia , Ochieng Peter Juma
Published: January 2023

Farmers’ satisfaction with new superior varieties (NSVs) is a critical strategy for boosting their adoption. Out of 48 national NSV hybrids produced, only three, including Nasa-29, JH-37, and Bima-10, have been widely distributed at the farmer level. However, no studies have been carried out to establish farmers’ satisfaction of any of the three hybrid maize varieties. As a result, the main aim of this study is to establish farmers’ satisfaction of three-hybrid maize. The survey was conducted in three South Sulawesi maize production districts: Bone, Gowa, and North Luwu, representing the east, west, and transitional zones. A total of 150 farmers from three districts were then deliberately chosen as respondents. Variables such as seed availability, cultivation technology, post-harvest, and product marketing were monitored during our assessment. The data were then analyzed using the importance performance analysis (IPA) method. The findings indicate that seed quality and quantity, disease resistance, low yield, and productivity are the variables that require intervention to improve farmer satisfaction with the superiority of the national hybrid maize NSV. The low price of seeds, the ability to grow at 15 days, the small size of the cob, ease of harvest, and the accessibility of marketing the product at the best price were then deemed adequate variables.
Weed Identification in Soybean Seedling Stage Based on Optimized Faster R-CNN Algorithm
Authors: Zhang Xinle , Cui Jian , Liu Huanjun , Han Yongqi , Ai Hongfu , Dong Chang , Zhang Jiaru , Chu Yunxiang
Published: January 2023

Soybean in the field has a wide range of intermixed weed species and a complex distribution status, and the weed identification rate of traditional methods is low. Therefore, a weed identification method is proposed based on the optimized Faster R-CNN algorithm for the soybean seedling. Three types of weed datasets, including soybean, with a total of 9816 photos were constructed, and cell phone photo data were used for training and recognition. Firstly, by comparing the classification effects of ResNet50, VGG16, and VGG19, VGG19 was identified as the best backbone feature extraction network for model training. Secondly, an attention mechanism was embedded after the pooling layer in the second half of VGG19 to form the VGG19-CBAM structure, which solved the problem of low attention to the attention target during model training using the trained Faster R-CNN algorithm to identify soybean and weeds in the field under the natural environment and compared with two classical target detection algorithms, SSD and Yolov4. The experimental results show that the Faster R-CNN algorithm using VGG19-CBAM as the backbone feature extraction network can effectively identify soybeans and weeds in complex backgrounds. The average recognition speed for a single image is 336 ms, and the average recognition accuracy is 99.16%, which is 5.61% higher than before optimization, 2.24% higher than the SSD algorithm, and 1.24% higher than the Yolov4 algorithm. Therefore, this paper’s optimized target detection model is advantageous and can provide a scientific method for accurate identification and monitoring of grass damage.
Authenticity Analysis of Cold-Pressed Orange Essential Oils by GC/MS on Polymethoxyflavone Components
Authors: Yang Kai-Min , Chen Chih-Wei , Chen Min-Hung , Chen Hsin-Chun , Lin Li-Yun
Published: January 2023

Citrus essential oil combines numerous components with many qualities and control issues. For example, how to monitor volatile components and nonvolatile substances simultaneously is a major problem. Therefore, the objective of this study was to develop a Gas chromatography/mass spectrometry (GC/MS) method for the compositional study of total constituents in cold-pressed essential oils from major orange species. A polysiloxane GC column (DB-1, nonpolar, low-bleeding) was used in this study; its fingerprint includes terpenoids (Section 1), long-chain hydrocarbons (Section 2), phytosterols, and polymethoxyflavones (Section 3). These markers are more effective in detecting adulteration of essential oil products than volatile components, and more effective than quantitative PMF by 5α-Cholestane for establishing authenticity. The study aims to use the analysis procedure as a routine quality control test for authenticity evaluation in cold-pressed orange essential oils (CP-OrEO).
Comparison of Weather Acquisition Periods Influencing a Statistical Model of Aerial Pesticide Drift
Authors: Thomson Steven J. , Huang Yanbo
Published: January 2023

Off-target drift of crop protection materials from aerial spraying can be detrimental to sensitive crops, beneficial insects, and the environment. So, it is very important to accurately characterize weather effects for accurate recommendations on drift mitigation. Wind is the single-most important weather factor influencing localized off-target drift of crop protection materials. In drift sampling experiments, it is difficult to accurately characterize wind speed and direction at a drift sampling location, owing to the natural variability of spray movement on the way to the sampling target. Although it is difficult or impossible to exactly track wind movement to a target, much information can be gained by altering the way wind speed and tracking is characterized from field experiments and analyzed using statistical models of spray drift. In this study two methods of characterizing weather were compared to see how they affect results from a statistical model of downwind spray drift using field data. Use of a method that implemented weather averages over the length of a spray run resulted in model-based estimates for spray tracer concentration that compared well with field data averages. Model results using this method showed only a slight sensitivity to changes in wind speed, and this difference was more pronounced further downwind. The degree of this effect was consistent with field results. Another method that used single weather values obtained at the beginning of each run resulted in an unexpected inverse relationship of residue concentration with respect to increases in wind speed by sensitivity analysis and would thus not be recommended for use in a statistical model of downwind spray drift. This study could provide a guideline for general agricultural aviation analysis and unmanned aerial vehicle spray application studies.
Structural Basis for the IgE-Binding Cross-Reacting Epitopic Peptides of Cup s 3, a PR-5 Thaumatin-like Protein Allergen from Common Cypress (Cupressus sempervirens) Pollen
Authors: Barre Annick , Sénéchal Hélène , Nguyen Christophe , Granier Claude , Poncet Pascal , Rougé Pierre
Published: January 2023

The present work was aimed at identifying the IgE-binding epitopic regions on the surface of the Cup s 3 allergen from the common cypress Cupressus sempervirens, that are possibly involved in the IgE-binding cross-reactivity reported between Cupressaceae species. Three main IgE-binding epitopic regions were mapped on the molecular surface of Cup s 3, the PR-5 thaumatin-like allergen of common cypress Cupressus sempervirens. They correspond to exposed areas containing either electropositive (R, K) or electronegative (D, E) residues. A coalescence occurs between epitopes #1 and #2, that creates an extended IgE-binding regions on the surface of the allergen. Epitope #3 contains a putative N-glycosylation site which is actually glycosylated and could therefore comprise a glycotope. However, most of the allergenic potency of Cup s 3 depends on non-glycosylated epitopic peptides. The corresponding regions of thaumatin-like allergens from other closely related Cupressaceae (Cryptomeria, Juniperus, Thuja) exhibit a very similar conformation that should account for the IgE-binding cross-reactivity observed among the Cupressaceae allergens.
Evaluation of an Oral Supplemental Cannabidiol Product for Acceptability and Performance in Mature Horses
Authors: Leise Julia M. , Leatherwood Jessica L. , Paris Brittany L. , Walter Kelly W. , George James M. , Martinez Rafael E. , Glass Kati P. , Lo Chih-Ping , Mays Travis P. , Wickersham Tryon A.
Published: January 2023

Thirty stock type geldings (15 ± 3 years; 556 ± 63 kg BW) were used in a randomized complete design over 28 days to determine the influence of cannabidiol (CBD) oil supplementation levels on body weight, body condition, and blood chemistry. Horses were randomly assigned to one of three dietary treatments (n = 10 per treatment) formulated with canola oil to provide 1.50 mg CBD/kg BW (TRTA), 0.75 mg CBD/kg BW (TRTB), or 0.00 mg CBD/kg BW (canola oil; CTRL). Treatments were top-dressed onto concentrate and individually administered twice daily. Horses were maintained in adjacent dry lots and received coastal bermudagrass hay ad libitum. Body weight and body condition scores (BCS) were obtained every 14 days. On day 0 and 28, blood was collected via jugular venipuncture and serum was harvested to perform a blood chemistry panel and drugs of abuse screening at the Texas Veterinary Medical Diagnostic Laboratory. Data were analyzed using PROC MIXED of SAS (v9.4), and the model included treatment, time, and the treatment × time interaction, and linear and quadratic orthogonal polynomial contrasts to partition sum of squares. Analysis of composited treatment samples revealed lower CBD concentrations than indicated from initial testing by the manufacturer (0.13 mg CBD/kg in TRTA; 0.12 mg CBD/kg in TRTB). At this level of supplementation, canola-based CBD oil was well-accepted by mature horses, banned substances were not detectable in blood, and blood chemistry parameters were not adversely affected as a result of supplementation. More research is warranted to describe the discrepancy between formulated levels compared to tested levels of CBD in the canola-based supplement.
Chimpanzees (Pan troglodytes) in U.S. Zoos, Sanctuaries, and Research Facilities: A Survey-Based Comparison of Species-Typical Behaviors
Authors: Clay Andrea W. , Ross Stephen R. , Lambeth Susan , Vazquez Maribel , Breaux Sarah , Pietsch Rhonda , Fultz Amy , Lammey Michael , Jacobson Sarah L. , Perlman Jaine E. , Bloomsmith Mollie A.
Published: January 2023

A survey was sent to zoos, research facilities, and sanctuaries which housed chimpanzees. Data collected included information about 1122 chimpanzees’ age, sex, social group-size, rearing history, and enclosure. Respondents were also asked to indicate if certain behaviors had been observed in each chimpanzee over the prior two years. Species- typical behaviors (STBs) were queried, including copulation, tool-use, nest-building, and social grooming. Tool-use was reported present for 94.3% of the sample (n = 982), active social grooming for 85.7% (n = 1121), copulation for 68.3% (n = 863) and nest-building for 58.9% (n = 982). Of the subjects for whom we had data regarding all four STBs (n = 860), 45.6% were reported to engage in all four. Logistic regression analyses using forward Wald criteria were conducted to determine the best model for each STB based on the predictors of age, sex, rearing history, group-size, facility-type, and a sex-by-rearing interaction. The best model for copulation (χ2(6) = 124.62, p < 0.001) included rearing, group-size, facility-type, and the sex-by-rearing interaction. Chimpanzees were more likely to copulate if they were mother-reared, in larger groups, living in research facilities, and, if not mother-reared (NOTMR), more likely to copulate if they were female. The best model for tool-use retained the predictors of age category, facility-type, and sex-by-rearing (χ2(5) = 55.78, p < 0.001). Chimpanzees were more likely to use tools if they were adult, living in research facilities, and if NOTMR, were female. The best model for nest-building included facility-type and rearing (χ2(3) = 205.71, p < 0.001). Chimpanzees were more likely to build nests if they were MR and if they were living in zoos or in sanctuaries. The best model for active social grooming retained the predictors of age, sex, rearing, and type of facility (χ2(6) = 102.15, p < 0.001). Chimpanzees were more likely to engage in active social grooming if they were immature, female, mother-reared, and living in zoos. This research provides a basic behavioral profile for many chimpanzees living under human care in the United States and allows us to determine potential methods for improving the welfare of these and future chimpanzees in this population.
Embedded-AMP: A Multi-Thread Computational Method for the Systematic Identification of Antimicrobial Peptides Embedded in Proteome Sequences
Authors: Carballo Germán Meléndrez , Vázquez Karen Guerrero , García-González Luis A. , Rio Gabriel Del , Brizuela Carlos A.
Published: January 2023

Antimicrobial peptides (AMPs) have gained the attention of the research community for being an alternative to conventional antimicrobials to fight antibiotic resistance and for displaying other pharmacologically relevant activities, such as cell penetration, autophagy induction, immunomodulation, among others. The identification of AMPs had been accomplished by combining computational and experimental approaches and have been mostly restricted to self-contained peptides despite accumulated evidence indicating AMPs may be found embedded within proteins, the functions of which are not necessarily associated with antimicrobials. To address this limitation, we propose a machine-learning (ML)-based pipeline to identify AMPs that are embedded in proteomes. Our method performs an in-silico digestion of every protein in the proteome to generate unique k-mers of different lengths, computes a set of molecular descriptors for each k-mer, and performs an antimicrobial activity prediction. To show the efficiency of the method we used the shrimp proteome, and the pipeline analyzed all k-mers between 10 and 60 amino acids in length to predict all AMPs in less than 20 min. As an application example we predicted AMPs in different rodents (common cuy, common rat, and naked mole rat) with different reported longevities and found a relation between species longevity and the number of predicted AMPs. The analysis shows as the longevity of the species is higher, the number of predicted AMPs is also higher. The pipeline is available as a web service.
Prevalence of Carbapenemase and Extended-Spectrum β-Lactamase Producing Enterobacteriaceae: A Cross-Sectional Study
Authors: Mustafai Muhammad Muqaddas , Hafeez Mavra , Munawar Safa , Basha Sakeenabi , Rabaan Ali A. , Halwani Muhammad A. , Alawfi Abdulsalam , Alshengeti Amer , Najim Mustafa A. , Alwarthan Sara , AlFonaisan Meshal K. , Almuthree Souad A. , Garout Mohammed , Ahmed Naveed
Published: January 2023

Enterobacteriaceae have been classified as severely drug resistant bacteria by the World Health Organization due to their extensive production and dissemination of carbapenemases (CPs) and extended-spectrum β-lactamases (ESBL). The current study was conducted with the aim to determine the prevalence of CP- and ESBL-producing Enterobacteriaceae, as well as their antibiotic susceptibility profiles. For this, a hospital-based study was conducted which included 384 participants with bacterial infections. The collection and processing of specimens was conducted per standard microbiological protocol. The samples were inoculated on agar media plates to obtain the bacterial growths, and if they were positive for any bacterial growth, the antibiotic susceptibility testing was performed using disk diffusion method to check their antibiotic susceptibility patterns. The double disc diffusion as well as carbapenem inhibition techniques were used to examine the CP enzymes. Multiplex real-time PCR technique was performed to identify three distinct genetic types of CPs that have been identified in the Enterobacteriaceae (KPC, NDM, and OXA-48). A majority of participants (58.3%) in the current study were living in urban areas. A total of 227 (59.1%) patients were hospitalized. Furthermore, 26.04% of the patients were determined to be suffering from infections with Enterobacteriaceae. Escherichia coli was the most prevalent (9.1%) isolate overall, followed by Klebsiella pneumoniae (8.07%), Acinetobacter baumannii (2.6%), Pseudomonas aeruginosa (3.1%), Enterobacter cloacae (1.3%), Proteus spp. (1.3%), and Morganella spp. (0.5%). The studied patients were suffering from urinary tract infections (48.6%), blood stream infections (32.2%), wounds infection (11.9%), and respiratory infections (7.03%), confirmed with bacterial cultures. The resistance against carbapenems was seen in 31.4% of E. coli isolates, 25.8% in K. pneumoniae, 50% in P. aeruginosa, 25% in A. baumannii, and 20% in E. cloacae isolates. Such high rates of CP- and ESBL-producing Enterobacteriaceae are alarming, suggesting high spread in the study area. It is advised to implement better infection prevention and control strategies and conduct further nationwide screening of the carriers of these pathogens. This might help in reducing the burden of highly resistant bugs.
Prevalence of Hypertension and Obesity: Profile of Mitochondrial Function and Markers of Inflammation and Oxidative Stress
Authors: García-Sánchez Andrés , Gómez-Hermosillo Luis , Casillas-Moreno Jorge , Pacheco-Moisés Fermín , Campos-Bayardo Tannia Isabel , Román-Rojas Daniel , Miranda-Díaz Alejandra Guillermina
Published: January 2023

Obesity and hypertension are health problems of increasing prevalence in developed countries. The link between obesity and hypertension is not yet fully determined. Oxidative stress (OS) and mitochondrial function may play a role in obesity-associated hypertension. A cross-sectional study with 175 subjects with normal weight, overweight, or obese who attended a medical check-up was included. The subjects were divided according to the body mass index (BMI) into normal-weight (n-53), overweight (n-84), and obesity (n-38). Hypertension was also evaluated. To measure mitochondrial function, ATP hydrolysis and ATP synthesis in platelets and serum, respectively, were determined. Superoxide dismutase (SOD), catalase, lipohydroperoxides, 8-isoprostanes, carbonyl groups in proteins, nitric oxide (NO) metabolites, 8-hydroxy-2′-deoxyguanosine (8-OHG), 8-oxoguanine glycosylase (hOGG1), tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) were measured by standard colorimetric or immunoassay methods. Obese subjects showed lower ATP hydrolysis activity than normal weight and overweight subjects (p < 0.01). No differences between those groups were found in ATP synthase and catalase activities, lipid hydroperoxides, carbonyl groups in proteins, 8-isoprostanes, and NO metabolites. In the obesity group, SOD activity (p < 0.01) was decreased while 8-OHG (p < 0.01) was increased. Subjects with hypertension showed increased 8-OHG (p < 0.01) and less reparative enzyme (hOGG1 p = 0.04) than subjects with normal weight. Moreover, we found a decrease of SOD (p < 0.01), catalase activities (p = 0.04), NO metabolites (p < 0.01), and increases of carbonyl groups in proteins (p = 0.01), TNF-α (p < 0.01) and IL-6 (p < 0.01 in hypertensive subjects. Obese subjects show a decrease in ATP hydrolysis. The decrease in ATP hydrolysis rate and ATP synthesis and an increase in OS and inflammation markers were associated with the hypertensive state.