bluuebunny/crossref_metadata_2025 huggingface dataset:
Discrete Fundamental AC Voltage Controller for Three-Phase Standalone Converters
Authors: García-Fernández Alejandro , Doval-Gandoy Jesús , Pérez-Estévez Diego
Published: January 2021

Voltage control of standalone converters with LC filter is usually based on proportional-resonant or proportional-integral controllers, which often require further active damping methods to achieve stability. These solutions place design constraints in the selection of the closed-loop pole locations which limit the achievable bandwidth and increase the design complexity. In contrast, in state-space based controllers, the closed-loop poles can be placed freely through state feedback, which makes them particularly suitable for high order plants and/or low sampling frequencies. Among the modern control methods, direct pole placement is a simple technique that enables the establishment of a straightforward relationship between outcome and design, as opposed to more advanced approaches. This paper presents a discrete state-space voltage controller for standalone converters with LC output filter. The proposed method combines the direct pole placement technique with a virtual disturbance observer in order to compensate the effects produced by the load and model mismatches. The design process takes into account both the filter parameters and the sampling frequency, rendering the performance of the obtained controller independent of both. The result is a streamlined design procedure that leads to consistent outcomes for a wide range of plant parameter variations, requiring only one input: the desired closed-loop bandwidth.
Assessment of Offshore Wave Energy Resources in Taiwan Using Long-Term Dynamically Downscaled Winds from a Third-Generation Reanalysis Product
Authors: Hsiao Shih-Chun , Cheng Chao-Tzuen , Chang Tzu-Yin , Chen Wei-Bo , Wu Han-Lun , Jang Jiun-Huei , Lin Lee-Yaw
Published: January 2021

In this study, long-term wind fields during 1991–2010 from the Climate Forecast System Reanalysis (CFSR) were dynamically downscaled over Taiwan and its offshore islands at a 5 km horizontal resolution using the Weather Research and Forecasting (WRF) model. Simulations of the 10 m (above sea level) dynamically downscaled winds served as the atmospheric forcing for driving a fully coupled wave-circulation model. The sea states of the waters surrounding Taiwan during 1991–2010 were hindcasted to evaluate the offshore wave energy resources and optimal wave energy hotspots. This study reveals that the southeastern offshore waters of Taiwan and the Central Taiwan Strait exhibited the highest mean wave power density (WPD), exceeding 20 kW/m. The annual mean WPD, incidence of the hourly WPD greater than or equal to 4 kW/m, monthly variability index and coefficient of variation of the WPD indicated that the sea areas located between Green Island and Orchid Island (OH_1), southeast of Orchid Island (OH_2), south of the Hengchun Peninsula (OH_3), and north of the Penghu Islands (OH_4) were the optimal hotspots for deploying wave energy converters. The most energetic months were October for OH_1 and OH_2 and November for OH_3 and OH_4, while the wave power was weak from March to June for OH_1, OH_2 and OH_3 and in May for OH_4. The wave direction is prevailingly east-northeast for OH_1, OH_2 and OH_3 and nearly northeast for OH_4. These phenomena reveal that wave power in the waters offshore Taiwan is induced primarily by the northeast (winter) monsoon. The exploitable annual WPD was estimated to be 158.06, 182.89, 196.39 and 101.33 MWh/m for OH_1, OH_2, OH_3 and OH_4, respectively.
Dynamic Prioritization of Functions during Real-Time Multi-Use Operation of Battery Energy Storage Systems
Authors: Marchgraber Jürgen , Gawlik Wolfgang
Published: January 2021

Battery Energy Storage Systems (BESS) based on Li-Ion technology are considered to be one of the providers of services in the future power system. Although prices for Li-Ion batteries are falling continuously, it is still difficult to achieve profitability from a single service today. Multi-use operation of BESS in order to reach a so-called “value-stacking” of services therefore is a hotly debated topic in literature, since such an operation holds the potential to increase profitability dramatically. The multi-use operation of a BESS can be divided into two parts: the operational planning phase and the real-time operation. While the operational planning phase has been examined in many studies, there seems to be a lack of discussion for the real-time operation. This paper therefore tries to address the topic of the real-time operation in more detail. For this reason, this paper discusses concepts for implementing a real-time multi-use operation and introduces the novel concept of dynamic prioritization, which allows resolving conflicts of services. Besides the ability to cope with abnormal grid conditions, this concept also holds potential for a better utilization of resources during normal grid conditions. A mathematical framework is used to describe several services and their interaction, taking into account the concept of dynamic prioritization. Several applications are presented in order to demonstrate the behavior of the concept during normal and abnormal grid conditions. These applications are simulated in Matlab/Simulink for specific events and in the form of long-time simulations.
Stimulating E-Mobility Diffusion in Germany (EMOSIM): An Agent-Based Simulation Approach
Authors: Buchmann Tobias , Wolf Patrick , Fidaschek Stefan
Published: January 2021

The German Climate Action Plan targets an electric vehicle fleet of 6 million by 2030. However, from today’s perspective, we are far away from a path that is steep enough to reach this goal. In order to identify how different policy instruments can stimulate e-mobility diffusion in Germany, we build and calibrate an agent-based simulation model (ABM). The model allows for the consideration of the rich dynamics of social influence as well as the heterogeneity of actors and is flexible enough to be applied with other technologies. We simulate different policy scenarios against a business as usual (BAU) scenario. We show that with the currently implemented set of policies (BAU scenario), it is very unlikely that the envisaged goals in terms of e-mobility diffusion can be reached. Moreover, we suggest additional measures such as a carbon tax on fuel, more charging points, and higher direct subsidies, which are as a combined package likely to have a significantly positive effect on the diffusion of electric cars.
100% Renewable Energy Scenarios for North America—Spatial Distribution and Network Constraints
Authors: Zozmann Elmar , Göke Leonard , Kendziorski Mario , Rodriguez del Angel Citlali , von Hirschhausen Christian , Winkler Johanna
Published: January 2021

The urgency to combat climate change and the widely distributed, increasingly competitive renewable resources in North America are strong arguments to explore scenarios for a renewable energy supply in the region. While the current power system of North America is heavily dependent on fossil fuels, namely natural gas, coal and oil, and some nuclear power plants, some current policies at the state level, and future federal policies are likely to push the share of different renewable sources available in Mexico, the U.S., and Canada. This paper explores three scenarios for a renewable energy supply, using a bottom-up energy system model with a high level of spatial and time granularity. The scenarios span the extremes with respect to connecting infrastructure: while one scenario only looks at state-level supply and demand, without interconnections, the other extreme scenario allows cross-continental network investments. The model results indicate that the North American continent (a) has sufficient renewable potential to satisfy its energy demand with renewables, independent of the underlying grid assumption, (b) solar generation dominates the generation mix as the least-cost option under given renewable resource availability and (c) simultaneous planning of generation and transmission capacity expansion does not result in high grid investments, but the necessary flexibility to integrate intermittent renewable generation is rather provided by the existing grid in combination with short-term and seasonal storages.
Implications of Nonlinear Material Parameters on the Dielectric Loss under Harmonic Distorted Voltages
Authors: Linde Thomas , Loh Jun Ting , Kornhuber Stefan , Backhaus Karsten , Schlegel Stephan , Großmann Steffen
Published: January 2021

The increasing number of power electronic devices in electrical grids may lead to harmonic distorted voltages, which are considered to be possible causes of component failures. In this study, the dielectric losses of epoxy polymer and functionally filled silicone rubber (f-SiR) samples are investigated under harmonic distorted voltages in terms of their dielectric losses and the consequent heat source density within the material. The results suggest that the epoxy polymer samples behave linearly to the electric field strength and therefore allow good predictability of the dielectric losses. The investigated f-SiR samples exhibit a nonlinear behaviour when an electric field threshold is exceeded. The subsequent direct loss measurements under harmonic distorted voltage reveal a sharp rise of the dielectric loss with increasing electrical field strength. This leads to a higher risk of excessive heat in the material when harmonics are present. In conclusion, the investigation highlights the difficulties of estimating dielectric losses in nonlinear dielectric materials when distorted voltages are present.
Microbiome of Seven Full-Scale Anaerobic Digestion Plants in South Korea: Effect of Feedstock and Operational Parameters
Authors: Sposob Michal , Moon Hee-Sung , Lee Dongjin , Yun Yeo-Myeong
Published: January 2021

In this study, the microbiomes linked with the operational parameters in seven mesophilic full-scale AD plants mainly treating food waste (four plants) and sewage sludge (three plants) were analyzed. The results obtained indicated lower diversity and evenness of the microbial population in sludge digestion (SD) plants compared to food digestion (FD) plants. Candidatus Accumulibacter dominated (up to 42.1%) in SD plants due to microbial immigration from fed secondary sludge (up to 89%). Its potential activity in SD plants was correlated to H2 production, which was related to the dominance of hydrogenotrophic methanogens (Methanococcus). In FD plants, a balance between the hydrogenotrophic and methylotrophic pathways was found, while Flavobacterium and Levilinea played an important role during acidogenesis. Levilinea also expressed sensitivity to ammonia in FD plants. The substantial differences in hydraulic retention time (HRT), organic loading rate (OLR), and total ammonium nitrogen (TAN) among the studied FD plants did not influence the archaeal methane production pathway. In addition, the bacterial genera responsible for acetate production through syntrophy and homoacetogenesis (Smithella, Treponema) were present in all the plants studied.
Protection Method Based on Wavelet Entropy for MMC-HVDC Overhead Transmission Lines
Authors: Huang Weibo , Luo Guomin , Cheng Mengxiao , He Jinghan , Liu Zhao , Zhao Yuhong
Published: January 2021

Recent technological developments in modular multilevel converter-based high-voltage direct current (MMC-HVDC) transmission systems have shown significant advantages over the traditional HVDC and two-level voltage source converter (VSC) transmission systems. However, there are a lack of studies on the protection methods for MMC-HVDC overhead lines where the protection method should be able to provide a fast and accurate response and be able to identify lightning strikes. In this paper, a wavelet entropy-based protection method is proposed. Due to the capability of revealing time–frequency distribution features, the proposed protection method combines wavelet and entropy to identify the time–frequency characteristics of different faults. Simulation results show that the proposed method can accurately and quickly determine the types of faults or disturbances with appropriate noise tolerance. In addition, the impact of the ground resistor and fault distance on the performance of the proposed method is studied.
Does Rheology of Bingham Fluid Influence Upscaling of Flow through Tight Porous Media?
Authors: Liu Tong , Zhang Shiming , Wang Moran
Published: January 2021

Non-Newtonian fluids may cause nonlinear seepage even for a single-phase flow. Through digital rock technologies, the upscaling of this non-Darcy flow can be studied; however, the requirements for scanning resolution and sample size need to be clarified very carefully. This work focuses on Bingham fluid flow in tight porous media by a pore-scale simulation on CT-scanned microstructures of tight sandstones. A bi-viscous model is used to depict the Bingham fluid. The results show that when the Bingham fluid flows through a rock sample, the flowrate increases at a parabolic rate when the pressure gradient is small and then increases linearly with the pressure gradient. As a result, an effective permeability and a start-up pressure gradient can be used to characterize this flow behavior. By conducting flow simulations at varying sample sizes, we obtain the representative element volume (REV) for effective permeability and start-up pressure gradient. It is found that the REV size for the effective permeability is almost the same as that for the absolute permeability of Newtonian fluid. The interesting result is that the REV size for the start-up pressure gradient is much smaller than that for the effective permeability. The results imply that the sample size, which is large enough to reach the REV size for Newtonian fluids, can be used to investigate the Bingham fluids flow through porous media as well.
An Improved Analytical Model for Crosstalk of SiC MOSFET in a Bridge-Arm Configuration
Authors: Yuan Dakang , Zhang Yiming , Wang Xuhong
Published: January 2021

SiC MOSFETs have an excellent characteristic of high switching speed, which can improve the efficiency and power density of converters significantly. However, the fast switching processes of SiC MOSFETs cause serious crosstalk problems in bridge-arm configurations, which restricts the devices’ performances. This paper presents a detailed and accurate improved crosstalk analytical model, which takes into account the nonlinear capacitances, the parasitic inductances, the reverse recovery characteristics of the anti-parallel diodes, and the nonlinear voltage switching and damping oscillation process. The novelty of the proposed model lies in the fact that under the condition of comprehensively considering all these non-ideal factors of the bridge-arm, the effects of multi-parasitic elements and multi-variables coupling to the crosstalk are hierarchically divided. The parasitic elements and their correlations are described in detail and the direct and indirect variables’ impacts are clearly traced. Thus, according to the different variables switching stages, the influence processes of these parasitic elements and variables can be integrated and a complete equivalent analytical model of the crosstalk process can be derived. The simulation and experiment platforms are established and a series of experimental verifications and comparisons prove that the model can replicate experimental measurements of crosstalk with good accuracy and detail.
Soil Bacterial Community Responds to Land-Use Change in Riparian Ecosystems
Authors: Waymouth Vicky , Miller Rebecca E. , Kasel Sabine , Ede Fiona , Bissett Andrew , Aponte Cristina
Published: January 2021

Riparian forests were frequently cleared and converted to agricultural pastures, but in recent times these pastures are often revegetated in an effort to return riparian forest structure and function. We tested if there is a change in the soil bacterial taxonomy and function in areas of riparian forest cleared for agricultural pasture then revegetated, and if soil bacterial taxonomy and function is related to vegetation and soil physicochemical properties. The study was conducted in six riparian areas in south-eastern Australia, each comprising of three land-use types: remnant riparian forest, cleared forest converted to pasture, and revegetated pastures. We surveyed three strata of vegetation and sampled surface soil and subsoil to characterize physicochemical properties. Taxonomic and functional composition of soil bacterial communities were assessed using 16S rRNA gene sequences and community level physiological profiles, respectively. Few soil physiochemical properties differed with land use despite distinct vegetation in pasture relative to remnant and revegetated areas. Overall bacterial taxonomic and functional composition of remnant forest and revegetated soils were distinct from pasture soil. Land-use differences were not consistent for all bacterial phyla, as Acidobacteria were more abundant in remnant soils; conversely, Actinobacteria were more abundant in pasture soils. Overall, bacterial metabolic activity and soil carbon and nitrogen content decreased with soil depth, while bacterial metabolic diversity and evenness increased with soil depth. Soil bacterial taxonomic composition was related to soil texture and soil fertility, but functional composition was only related to soil texture. Our results suggest that the conversion of riparian forests to pasture is associated with significant changes in the soil bacterial community, and that revegetation contributes to reversing such changes. Nevertheless, the observed changes in bacterial community composition (taxonomic and functional) were not directly related to changes in vegetation but were more closely related to soil attributes.
Exploring the Influence of Biological Traits and Environmental Drivers on Water Use Variations across Contrasting Forests
Authors: Zhao Xiuhua , Zhao Ping , Zhu Liuwei , Wang Qian , Hu Yanting , Cranston Benjamin M. , Kaplick Julia , Lei Ouyang , Chen Xia , Ni Guangyan , Ye Qing , Macinnis-Ng Cate
Published: January 2021

Understanding species-specific water use patterns across contrasting sites and how sensitivity of responses to environmental variables changes for different species is critical for evaluating potential forest dynamics and land use changes under global change. To quantify water use patterns and the sensitivity of tree transpiration to environmental drivers among sites and species, sap flow and meteorological data sets from three contrasting climatic zones were combined and compared in this analysis. Agathis australis from NZHP site, Schima wallichii Choisy (native) and Acacia mangium Willd (exotic) from CHS site, Liquidamber formosana Hance, Quercus variabilis Blume and Quercus acutissima Carruth from CJGS site were the dominant trees chosen as our study species. Biological traits were collected to explain the underlying physiological mechanisms for water use variation. Results showed that the strongest environmental drivers of sap flow were photosynthetically active radiation (PAR), vapor pressure deficit (VPD) and temperature across sites, indicating that the response of water use to abiotic drivers converged across sites. Water use magnitude was site specific, which was controlled by site characteristics, species composition and local weather conditions. The species with higher sap flow density (Fd) generally had greater stomatal conductance. Native deciduous broadleaved species had a higher Fd and faster response to stomatal regulation than that of native evergreen broadleaved species (S. wallichii) and conifer species A. australis. The analysis also showed that exotic species (A. mangium) consumed more water than native species (S. wallichii). Trees with diffuse porous and lower wood density had relatively higher Fd for angiosperms, suggesting that water use was regulated by physiological differences. Water use characteristics across sites are controlled by both external factors such as site-specific characteristics (local environmental conditions and species composition) and internal factors such as biological traits (xylem anatomy, root biomass and leaf area), which highlights the complexity of quantifying land water budgets for areas covered by different species.
A Novel Approach to Evaluate the Effect of Neighboring Trees and the Orientation of Tree Social Area on Stem Radial Increment of Norway Spruce Trees
Authors: Světlík Jan , Krejza Jan , Bednář Pavel
Published: January 2021

Tree growth depends on many factors such as microsite conditions, vitality, and variations in climate and genetics. It is generally accepted that higher growth indicates both an economic benefit and better vitality of any tree. Here we use a modified approach of evaluating tree social area to study mutual tree competition based on the orientation and shape of trees social area. The investigation was performed in nine Norway spruce stands in the Czech Republic. The objective of this study performed from 2008 to 2012 was to quantify relative tree radial increments with respect to the lowest and highest competition found in specific sectors of tree social area (AS). Specific groups of trees (tree classes) were evaluated according to their classes (dominant, co-dominant and sub-dominant) and their composition status in ninety-degree sectors of AS using established classifying rules. The results showed that a spatially-available area (AA) is an inappropriate parameter for predicting tree growth, whereas AS provided robust explanatory power to predict relative radial growth. Tree size was observed as an important indicator of relative radial increments. A significantly positive correlation was found for a radial increment of sub-dominant trees with the lowest competition from western directions; whereas a negative correlation was observed when the lowest competition was observed from eastern directions. For dominant trees, there was an evident growth reaction only when more than 50% of the AS was oriented towards one of the cardinal points. Individual differences in the orientation of tree AS may be important parameters with regard to competition and its spatial variability within an area surrounding a particular tree and deserve more detailed attention in tree growth models and practice.
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-Source
Authors: Gallardo-Benavente Carla , Campo-Giraldo Jessica L. , Castro-Severyn Juan , Quiroz Andrés , Pérez-Donoso José M.
Published: January 2021

Here, we present the draft genome sequence of Pseudomonas sp. GC01, a cadmium-resistant Antarctic bacterium capable of biosynthesizing CdS fluorescent nanoparticles (quantum dots, QDs) employing a unique mechanism involving the production of methanethiol (MeSH) from methionine (Met). To explore the molecular/metabolic components involved in QDs biosynthesis, we conducted a comparative genomic analysis, searching for the genes related to cadmium resistance and sulfur metabolic pathways. The genome of Pseudomonas sp. GC01 has a 4,706,645 bp size with a 58.61% G+C content. Pseudomonas sp. GC01 possesses five genes related to cadmium transport/resistance, with three P-type ATPases (cadA, zntA, and pbrA) involved in Cd-secretion that could contribute to the extracellular biosynthesis of CdS QDs. Furthermore, it exhibits genes involved in sulfate assimilation, cysteine/methionine synthesis, and volatile sulfur compounds catabolic pathways. Regarding MeSH production from Met, Pseudomonas sp. GC01 lacks the genes E4.4.1.11 and megL for MeSH generation. Interestingly, despite the absence of these genes, Pseudomonas sp. GC01 produces high levels of MeSH. This is probably associated with the metC gene that also produces MeSH from Met in bacteria. This work is the first report of the potential genes involved in Cd resistance, sulfur metabolism, and the process of MeSH-dependent CdS QDs bioproduction in Pseudomonas spp. strains.
InpactorDB: A Classified Lineage-Level Plant LTR Retrotransposon Reference Library for Free-Alignment Methods Based on Machine Learning
Authors: Orozco-Arias Simon , Jaimes Paula A. , Candamil Mariana S. , Jiménez-Varón Cristian Felipe , Tabares-Soto Reinel , Isaza Gustavo , Guyot Romain
Published: January 2021

Long terminal repeat (LTR) retrotransposons are mobile elements that constitute the major fraction of most plant genomes. The identification and annotation of these elements via bioinformatics approaches represent a major challenge in the era of massive plant genome sequencing. In addition to their involvement in genome size variation, LTR retrotransposons are also associated with the function and structure of different chromosomal regions and can alter the function of coding regions, among others. Several sequence databases of plant LTR retrotransposons are available for public access, such as PGSB and RepetDB, or restricted access such as Repbase. Although these databases are useful to identify LTR-RTs in new genomes by similarity, the elements of these databases are not fully classified to the lineage (also called family) level. Here, we present InpactorDB, a semi-curated dataset composed of 130,439 elements from 195 plant genomes (belonging to 108 plant species) classified to the lineage level. This dataset has been used to train two deep neural networks (i.e., one fully connected and one convolutional) for the rapid classification of these elements. In lineage-level classification approaches, we obtain up to 98% performance, indicated by the F1-score, precision and recall scores.
Miocene Seep-Carbonates of the Northern Apennines (Emilia to Umbria, Italy): An Overview
Authors: Conti Stefano , Argentino Claudio , Fioroni Chiara , Salocchi Aura Cecilia , Fontana Daniela
Published: January 2021

The natural emission of methane-rich fluids from the seafloor, known as cold seepage, is a widespread process at modern continental margins. The studies on present-day cold seepages provide high-resolution datasets regarding the fluid plumbing system, biogeochemical processes in the sediment, seafloor seepage distribution and ecosystems. However, the long-term (hundreds of thousands to millions of years) evolution of cold seepage remains elusive. The identification and study of outcrop analogous now exposed on land represent a valuable method for better understanding the effects of geological processes and climate forcing on the development of cold seepage systems. Here, we provide an overview on Miocene seep-carbonate deposits of the northern Apennines (from Emilia to the Umbria-Marchean sector, Italy), based on decades of field research integrated with detailed sedimentological and geochemical investigations. We report a total of 13 seep-carbonate outcrops, which formed in three different structural settings of the paleo-accretionary wedge corresponding to wedge-top basins, outer slope and intrabasinal highs at the deformational front. We discuss the recurring lithostratigraphic occurrence of seep deposits and the main compositional features (carbonate facies, carbon and oxygen stable isotopes) in order to interpret the seepage dynamics, duration and infer the contribution of methane-rich fluids released by paleo-gas hydrates. The datasets presented in this study represent a valuable complete record of cold seepage spanning ~12 Myr, that can be used to better understand factors controlling the regional-scale spatial and temporal evolution of cold seepage systems at modern active continental margins.
Evidence of Lithospheric Boudinage in the Grand Banks of Newfoundland from Geophysical Observations
Authors: MacDougall Malcolm D. J. , Braun Alexander , Fotopoulos Georgia
Published: January 2021

The evolution of the passive margin off the coast of Eastern Canada has been characterized by a series of rifting episodes which caused widespread extension of the lithosphere and associated structural anomalies, some with the potential to be classified as a result of lithospheric boudinage. Crustal thinning of competent layers is often apparent in seismic sections, and deeper Moho undulations may appear as repeating elongated anomalies in gravity and magnetic surveys. By comparing the similar evolutions of the Grand Banks and the Norwegian Lofoten-Vesterålen passive margins, it is reasonable to explore the potential of the same structures being present. This investigation supplements our knowledge of analogous examples in the Norwegian Margin and the South China Sea with a thorough investigation of seismic, gravity and magnetic signatures, to determine that boudinage structures are evident in the context of the Grand Banks. Through analysis of geophysical data (including seismic, gravity and magnetic observations), a multi-stage boudinage mechanism is proposed, which is characterized by an upper crust short-wavelength deformation ranging from approximately 20–80 km and a lower crust long-wavelength deformation exceeding 200 km in length. In addition, the boudinage mechanism caused slightly different structures which are apparent in the block geometry and layeredness. Based on these results, there are indications that boudinage wavelength increases with each successive rifting phase, with geometry changing from domino style to a more shearband/symmetrical style as the scale of deformation is increased to include the entire lithosphere.
Complete Androgen Insensitivity Syndrome: From Bench to Bed
Authors: Tyutyusheva Nina , Mancini Ilaria , Baroncelli Giampiero Igli , D’Elios Sofia , Peroni Diego , Meriggiola Maria Cristina , Bertelloni Silvano
Published: January 2021

Complete androgen insensitivity syndrome (CAIS) is due to complete resistance to the action of androgens, determining a female phenotype in persons with a 46,XY karyotype and functioning testes. CAIS is caused by inactivating mutations in the androgen receptor gene (AR). It is organized in eight exons located on the X chromosome. Hundreds of genetic variants in the AR gene have been reported in CAIS. They are distributed throughout the gene with a preponderance located in the ligand-binding domain. CAIS mainly presents as primary amenorrhea in an adolescent female or as a bilateral inguinal/labial hernia containing testes in prepubertal children. Some issues regarding the management of females with CAIS remain poorly standardized (such as the follow-up of intact testes, the timing of gonadal removal and optimal hormone replacement therapy). Basic research will lead to the consideration of new issues to improve long-term well-being (such as bone health, immune and metabolic aspects and cardiovascular risk). An expert multidisciplinary approach is mandatory to increase the long-term quality of life of women with CAIS.
The Role of GSH in Intracellular Iron Trafficking
Authors: Hider Robert , Aviles Mayra Vera , Chen Yu-Lin , Latunde-Dada Gladys Oluyemisi
Published: January 2021

Evidence is reviewed for the role of glutathione in providing a ligand for the cytosolic iron pool. The possibility of histidine and carnosine forming ternary complexes with iron(II)glutathione is discussed and the physiological significance of these interactions considered. The role of carnosine in muscle, brain, and kidney physiology is far from established and evidence is presented that the iron(II)-binding capability of carnosine relates to this role.
The Human Tissue-Engineered Cornea (hTEC): Recent Progress
Authors: Guérin Louis-Philippe , Le-Bel Gaëtan , Desjardins Pascale , Couture Camille , Gillard Elodie , Boisselier Élodie , Bazin Richard , Germain Lucie , Guérin Sylvain L.
Published: January 2021

Each day, about 2000 U.S. workers have a job-related eye injury requiring medical treatment. Corneal diseases are the fifth cause of blindness worldwide. Most of these diseases can be cured using one form or another of corneal transplantation, which is the most successful transplantation in humans. In 2012, it was estimated that 12.7 million people were waiting for a corneal transplantation worldwide. Unfortunately, only 1 in 70 patients received a corneal graft that same year. In order to provide alternatives to the shortage of graftable corneas, considerable progress has been achieved in the development of living corneal substitutes produced by tissue engineering and designed to mimic their in vivo counterpart in terms of cell phenotype and tissue architecture. Most of these substitutes use synthetic biomaterials combined with immortalized cells, which makes them dissimilar from the native cornea. However, studies have emerged that describe the production of tridimensional (3D) tissue-engineered corneas using untransformed human corneal epithelial cells grown on a totally natural stroma synthesized by living corneal fibroblasts, that also show appropriate histology and expression of both extracellular matrix (ECM) components and integrins. This review highlights contributions from laboratories working on the production of human tissue-engineered corneas (hTECs) as future substitutes for grafting purposes. It overviews alternative models to the grafting of cadaveric corneas where cell organization is provided by the substrate, and then focuses on their 3D counterparts that are closer to the native human corneal architecture because of their tissue development and cell arrangement properties. These completely biological hTECs are therefore very promising as models that may help understand many aspects of the molecular and cellular mechanistic response of the cornea toward different types of diseases or wounds, as well as assist in the development of novel drugs that might be promising for therapeutic purposes.
Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops
Authors: Razzaq Muhammad Khuram , Aleem Muqadas , Mansoor Shahid , Khan Mueen Alam , Rauf Saeed , Iqbal Shahid , Siddique Kadambot H. M.
Published: January 2021

Plants are regularly exposed to biotic and abiotic stresses that adversely affect agricultural production. Omics has gained momentum in the last two decades, fueled by statistical methodologies, computational capabilities, mass spectrometry, nucleic-acid sequencing, and peptide-sequencing platforms. Functional genomics—especially metabolomics, transcriptomics, and proteomics—have contributed substantially to plant molecular responses to stress. Recent progress in reverse and forward genetics approaches have mediated high-throughput techniques for identifying stress-related genes. Furthermore, web-based genetic databases have mediated bioinformatics techniques for detecting families of stress-tolerant genes. Gene ontology (GO) databases provide information on the gene product’s functional features and help with the computational estimation of gene function. Functional omics data from multiple platforms are useful for positional cloning. Stress-tolerant plants have been engineered using stress response genes, regulatory networks, and pathways. The genome-editing tool, CRISPR-Cas9, reveals the functional features of several parts of the plant genome. Current developments in CRISPR, such as de novo meristem induction genome-engineering in dicots and temperature-tolerant LbCas12a/CRISPR, enable greater DNA insertion precision. This review discusses functional omics for molecular insight and CRISPR-Cas9-based validation of gene function in crop plants. Omics and CRISPR-Cas9 are expected to garner knowledge on molecular systems and gene function and stress-tolerant crop production.
Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic
Authors: Arena Fabio , Pollini Simona , Rossolini Gian Maria , Margaglione Maurizio
Published: January 2021

Since early 2020, the COVID-19 pandemic has caused an excess in morbidity and mortality rates worldwide. Containment strategies rely firstly on rapid and sensitive laboratory diagnosis, with molecular detection of the viral genome in respiratory samples being the gold standard. The reliability of diagnostic protocols could be affected by SARS-CoV-2 genetic variability. In fact, mutations occurring during SARS-CoV-2 genomic evolution can involve the regions targeted by the diagnostic probes. Following a review of the literature and an in silico analysis of the most recently described virus variants (including the UK B 1.1.7 and the South Africa 501Y.V2 variants), we conclude that the described genetic variability should have minimal or no effect on the sensitivity of existing diagnostic protocols for SARS-CoV-2 genome detection. However, given the continuous emergence of new variants, the situation should be monitored in the future, and protocols including multiple targets should be preferred.
The Rice CHD3/Mi-2 Chromatin Remodeling Factor Rolled Fine Striped Promotes Flowering Independent of Photoperiod
Authors: Yoon Hyeryung , Shim Yejin , Yoo Soo-Cheul , Kang Kiyoon , Paek Nam-Chon
Published: January 2021

Genetic studies have revealed that chromatin modifications affect flowering time, but the underlying mechanisms by which chromatin remodeling factors alter flowering remain largely unknown in rice (Oryza sativa). Here, we show that Rolled Fine Striped (RFS), a chromodomain helicase DNA-binding 3 (CHD3)/Mi-2 subfamily ATP-dependent chromatin remodeling factor, promotes flowering in rice. Diurnal expression of RFS peaked at night under short-day (SD) conditions and at dawn under long-day (LD) conditions. The rfs-1 and rfs-2 mutants (derived from different genetic backgrounds) displayed a late-flowering phenotype under SD and LD conditions. Reverse transcription-quantitative PCR analysis revealed that among the flowering time-related genes, the expression of the major floral repressor Grain number and heading date 7 (Ghd7) was mainly upregulated in rfs mutants, resulting in downregulation of its downstream floral inducers, including Early heading date 1 (Ehd1), Heading date 3a (Hd3a), and Rice FLOWERING LOCUS T 1 (RFT1). The rfs mutation had pleiotropic negative effects on rice grain yield and yield components, such as plant height and fertility. Taking these observations together, we propose that RFS participates in multiple aspects of rice development, including the promotion of flowering independent of photoperiod.
Molecular Mechanism of Lipid Accumulation and Metabolism of Oleaginous Chlorococcum sphacosum GD from Soil under Salt Stress
Authors: Su Hang , Feng Jia , Lv Junping , Liu Qi , Nan Fangru , Liu Xudong , Xie Shulian
Published: January 2021

The oleaginous microalgae species Chlorococcum sphacosum GD is a promising feedstock for biodiesel production from soil. However, its metabolic mechanism of lipid production remains unclear. In this study, the lipid accumulation and metabolism mechanisms of Chlorococcum sphacosum GD were analyzed under salt stress based on transcriptome sequencing. The biomass and lipid content of the alga strain were determined under different NaCl concentrations, and total RNA from fresh cells were isolated and sequenced by HiSeq 2000 high throughput sequencing technology. As the salt concentration increased in culture medium, the algal lipid content increased but the biomass decreased. Following transcriptome sequencing by assembly and splicing, 24,128 unigenes were annotated, with read lengths mostly distributed in the 200–300 bp interval. Statistically significant differentially expressed unigenes were observed in different experimental groups, with 2051 up-regulated genes and 1835 down-regulated genes. The lipid metabolism pathway analysis showed that, under salt stress, gene-related fatty acid biosynthesis (ACCase, KASII, KAR, HAD, FATA) was significantly up-regulated, but some gene-related fatty acid degradation was significantly down-regulated. The comprehensive results showed that salt concentration can affect the lipid accumulation and metabolism of C. sphacosum GD, and the lipid accumulation is closely related to the fatty acid synthesis pathway.
Changes Induced by Mind–Body Intervention Including Epigenetic Marks and Its Effects on Diabetes
Authors: Yang Hyun-Jeong , Koh Eugene , Sung Min-Kyu , Kang Hojung
Published: January 2021

Studies have evidenced that epigenetic marks associated with type 2 diabetes (T2D) can be inherited from parents or acquired through fetal and early-life events, as well as through lifelong environments or lifestyles, which can increase the risk of diabetes in adulthood. However, epigenetic modifications are reversible, and can be altered through proper intervention, thus mitigating the risk factors of T2D. Mind–body intervention (MBI) refers to interventions like meditation, yoga, and qigong, which deal with both physical and mental well-being. MBI not only induces psychological changes, such as alleviation of depression, anxiety, and stress, but also physiological changes like parasympathetic activation, lower cortisol secretion, reduced inflammation, and aging rate delay, which are all risk factors for T2D. Notably, MBI has been reported to reduce blood glucose in patients with T2D. Herein, based on recent findings, we review the effects of MBI on diabetes and the mechanisms involved, including epigenetic modifications.
Deciphering the Role of Autophagy in Treatment of Resistance Mechanisms in Glioblastoma
Authors: Khan Imran , Baig Mohammad Hassan , Mahfooz Sadaf , Rahim Moniba , Karacam Busra , Elbasan Elif Burce , Ulasov Ilya , Dong Jae-June , Hatiboglu Mustafa Aziz
Published: January 2021

Autophagy is a process essential for cellular energy consumption, survival, and defense mechanisms. The role of autophagy in several types of human cancers has been explicitly explained; however, the underlying molecular mechanism of autophagy in glioblastoma remains ambiguous. Autophagy is thought to be a “double-edged sword”, and its effect on tumorigenesis varies with cell type. On the other hand, autophagy may play a significant role in the resistance mechanisms against various therapies. Therefore, it is of the utmost importance to gain insight into the molecular mechanisms deriving the autophagy-mediated therapeutic resistance and designing improved treatment strategies for glioblastoma. In this review, we discuss autophagy mechanisms, specifically its pro-survival and growth-suppressing mechanisms in glioblastomas. In addition, we try to shed some light on the autophagy-mediated activation of the cellular mechanisms supporting radioresistance and chemoresistance in glioblastoma. This review also highlights autophagy’s involvement in glioma stem cell behavior, underlining its role as a potential molecular target for therapeutic interventions.
NLRP3 Inflammasome: A New Pharmacological Target for Reducing Testicular Damage Associated with Varicocele
Authors: Antonuccio Pietro , Micali Antonio Girolamo , Romeo Carmelo , Freni Jose , Vermiglio Giovanna , Puzzolo Domenico , Squadrito Francesco , Irrera Natasha , Marini Herbert R. , Rana Rosa Alba , Pallio Giovanni , Minutoli Letteria
Published: January 2021

Many bioactive natural compounds are being increasingly used for therapeutics and nutraceutical applications to counteract male infertility, particularly varicocele. The roles of selenium and Polydeoxyribonucleotide (PDRN) were investigated in an experimental model of varicocele, with particular regard to the role of NLRP3 inflammasome. Male rats underwent sham operation and were daily administered with vehicle, seleno-L-methionine (Se), PDRN, and with the association Se-PDRN. Another group of rats were operated for varicocele. After twenty-eight days, sham and varicocele rats were sacrificed and both testes were weighted and analyzed. All the other rats were challenged for one month with the same compounds. In varicocele animals, lower testosterone levels, testes weight, NLRP3 inflammasome, IL-1β and caspase-1 increased gene expression were demonstrated. TUNEL assay showed an increased number of apoptotic cells. Structural and ultrastructural damage to testes was also shown. PDRN alone significantly improved all considered parameters more than Se. The Se-PDRN association significantly improved all morphological parameters, significantly increased testosterone levels, and reduced NLRP3 inflammasome, caspase-1 and IL-1β expression and TUNEL-positive cell numbers. Our results suggest that NLRP3 inflammasome can be considered an interesting target in varicocele and that Se-PDRN may be a new medical approach in support to surgery.
Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids
Authors: Ahmad Mulyadi Lai Henkie Isahwan , Chou Shih-Jie , Chien Yueh , Tsai Ping-Hsing , Chien Chian-Shiu , Hsu Chih-Chien , Jheng Ying-Chun , Wang Mong-Lien , Chiou Shih-Hwa , Chou Yu-Bai , Hwang De-Kuang , Lin Tai-Chi , Chen Shih-Jen , Yang Yi-Ping
Published: January 2021

Angiotensin-converting enzyme 2 (ACE2) was identified as the main host cell receptor for the entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its subsequent infection. In some coronavirus disease 2019 (COVID-19) patients, it has been reported that the nervous tissues and the eyes were also affected. However, evidence supporting that the retina is a target tissue for SARS-CoV-2 infection is still lacking. This present study aimed to investigate whether ACE2 expression plays a role in human retinal neurons during SARS-CoV-2 infection. Human induced pluripotent stem cell (hiPSC)-derived retinal organoids and monolayer cultures derived from dissociated retinal organoids were generated. To validate the potential entry of SARS-CoV-2 infection in the retina, we showed that hiPSC-derived retinal organoids and monolayer cultures endogenously express ACE2 and transmembrane serine protease 2 (TMPRSS2) on the mRNA level. Immunofluorescence staining confirmed the protein expression of ACE2 and TMPRSS2 in retinal organoids and monolayer cultures. Furthermore, using the SARS-CoV-2 pseudovirus spike protein with GFP expression system, we found that retinal organoids and monolayer cultures can potentially be infected by the SARS-CoV-2 pseudovirus. Collectively, our findings highlighted the potential of iPSC-derived retinal organoids as the models for ACE2 receptor-based SARS-CoV-2 infection.
Anti-Inflammatory and Proliferative Properties of Luteolin-7-O-Glucoside
Authors: De Stefano Alessandro , Caporali Sabrina , Di Daniele Nicola , Rovella Valentina , Cardillo Carmine , Schinzari Francesca , Minieri Marilena , Pieri Massimo , Candi Eleonora , Bernardini Sergio , Tesauro Manfredi , Terrinoni Alessandro
Published: January 2021

Flavonoids display a broad range of structures and are responsible for the major organoleptic characteristics of plant-derived foods and beverages. Recent data showed their activity, and in particular of luteolin-7-O-glucoside (LUT-7G), in reduction of oxidative stress and inflammatory mechanisms in different physiological systems. In this paper, we tried to elucidate how LUT-7G could exert both antioxidant and anti-inflammatory effects in endothelial cells cultured in vitro. Here, we showed that LUT-7G is able to inhibit the STAT3 pathway, to have an antiproliferative action, and an important antioxidant property in HUVEC cells. These properties are exerted by the flavone in endothelial through the transcriptional repression of a number of inflammatory cytokines and their receptors, and by the inhibition of ROS generation. ROS and STAT3 activation has been correlated with the production of oxysterols and other hydroxylated fatty acids, and they have been recognized important as players of atherogenesis and cardiocirculatory system diseases. The analysis of the general production pathway of these hydroxylated species, showed a strong decrease of cholesterol hydroxylated species such as 7-alpha-hydroxicholesterol, 7-beta-hydroxicholesterol by the treatment with LUT-7G. This confirms the anti-inflammatory properties of LUT-7G also in the endothelial district, showing for the first time the molecular pathway that verify previous postulated cardiovascular benefits of this flavone.