bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of ACE2 genetic variants in 131 Italian SARS-CoV-2-positive patients
Authors: Novelli Antonio , Biancolella Michela , Borgiani Paola , Cocciadiferro Dario , Colona Vito Luigi , D’Apice Maria Rosaria , Rogliani Paola , Zaffina Salvatore , Leonardis Francesca , Campana Andrea , Raponi Massimiliano , Andreoni Massimo , Grelli Sandro , Novelli Giuseppe
Published: September 2020

Background

Coronaviruses (CoV) are a large family of viruses that are common in humans and many animal species. Animal coronaviruses rarely infect humans with the exceptions of the Middle East respiratory syndrome (MERS-CoV), the severe acute respiratory syndrome corona virus (SARS-CoV), and now SARS-CoV-2, which is the cause of the ongoing pandemic of coronavirus disease 2019 (COVID-19). Several studies suggested that genetic variants in the ACE2 gene may influence the host susceptibility or resistance to SARS-CoV-2 infection according to the functional role of ACE2 in human pathophysiology. However, many of these studies have been conducted in silico based on epidemiological and population data. We therefore investigated the occurrence of ACE2 variants in a cohort of 131 Italian unrelated individuals clinically diagnosed with COVID-19 and in an Italian control population, to evaluate a possible allelic association with COVID-19, by direct DNA analysis.

Methods

As a pilot study, we analyzed, by whole-exome sequencing, genetic variants of ACE2 gene in 131 DNA samples of COVID-19 patients hospitalized at Tor Vergata University Hospital and at Bambino Gesù Children’s Hospital, Rome. We used a large control group consisting of 1000 individuals (500 males and 500 females).

Results

We identified three different germline variants: one intronic c.439+4G>A and two missense c.1888G>C p.(Asp630His) and c.2158A>G p.(Asn720Asp) in a total of 131 patients with a similar frequency in male and female. Thus far, only the c.1888G>C p.(Asp630His) variant shows a statistically different frequency compared to the ethnically matched populations. Therefore, further studies are needed in larger cohorts, since it was found only in one heterozygous COVID-19 patient.

Conclusions

Our results suggest that there is no strong evidence, in our cohort, of consistent association of ACE2 variants with COVID-19 severity. We might speculate that rare susceptibility/resistant alleles could be located in the non-coding regions of the ACE2 gene, known to play a role in regulation of the gene activity.
Association between personal values in adolescence and impaired bonding relationship with children
Authors: Obikane Erika , Watanabe Kazuhiro , Nishi Daisuke , Kawakami Norito
Published: September 2020

Background

Bonding disorders happen as parents cannot build an affective relationship with children and are associated with their psychological and social conditions. Personal values impact well-being and psychological outcomes; however, how personal values influence bonding relationships is unknown. The purpose of the study was to investigate the relationship between personal values in adolescence recalled by adult participants and impaired bonding using a community-representative database.

Methods

We conducted a retrospective study using cross-sectional data of adults living with children in Japan. Personal values were evaluated by value priorities measured by 11 items from Personal Value Questionnaires, and commitment to values measured by a Japanese version of the Personal Values Questionnaires II. Impaired bonding was evaluated by five items from a Japanese-version of the Mother-to-Infant Bonding Scale. Odds ratios of value priorities and commitment to values for impaired bonding relationships were calculated after adjusting covariates.

Results

Of 13,920 people selected by probability proportionate sampling, 466 participants with children under 6 years old were selected for analysis. Personal values on improving society, graduating from school, positive evaluation from others, and pursuing one’s interest were negatively associated with impaired bonding relationship, while personal values on financial success were positively associated with impaired bonding relationship. Commitment to values were negatively associated with bonding problems.

Conclusions

While a future longitudinal study is needed, the present findings may indicate that personal values in adolescence are associated with their bonding relationship with children.
1-MT inhibits the invasion of CBP-resistant ovarian cancer cells via down-regulating IDO expression and re-activating immune cells function
Authors: Ma Huihan , Qin Qian , Mi Jiaqing , Feng Qinmei
Published: September 2020

Background

The indoleamine 2, 3-dioxygenase (IDO) inhibitor 1-methyl-tryptophan (1-MT) is currently being used in clinical trials in patients with relapsed or refractory solid tumors by inhibiting tumor immune escape. A greater understanding of IDO activity is required to begin to understand the molecular mechanism by which drugs work. This study was conducted to investigate of the clinical significance of 1-methyl-tryptophan (1-MT) in treating carboplatin-resistant (CBP-resistant) ovarian cancer and its mechanism of action.

Methods

Using a medium dose, intermittent treatment method, a clinically relevant CBP-resistant human ovarian cancer cell line (SKOV3/CBP) was established. SKOV3/CBP cells were treated with normal serum (control) or 1-MT (0.25 ng/mL) for 4 h (SKOV3/CBP + 1-MT). Cell proliferation, invasion and IDO expression in SKOV3, SKOV3/CBP and SKOV3/CBP + 1-MT cells were determined by MTT assays, Matrigel invasion chambers assays and ELISAs, respectively. The half-maximal inhibitory concentration (IC50) and resistance index (RI) were also calculated. The killing ability of the NK cells and CD8+ T cells co-cultured with SKOV3, SKOV3/CBP and SKOV3/CBP + 1-MT cells were determined by LDH activity assays and the INF-γcounting method.

Results

The SKOV3/CBP cell line displayed an increased IC50 compared to the SKOV3 cell line (P < 0.05) under CBP treatment. Treatment with 1-MT significantly decreased the IC50 and RI of SKOV3/CBP cells. Furthermore, 1-MT treatment not only reduced the invasion ability, but also suppressed IDO expression in the drug-resistant SKOV3/CBP + 1-MT cell line as compared to the SKOV3/CBP cell line. Furthermore, 1-MT enhanced the killing ability of NK cells and the amount of INF-γsecreted from CD8+ T cells which were co-cultured with the SKOV3/CBP cell line.

Conclusion

Our data suggested that 1-MT inhibits the invasion of CBP-resistant ovarian cancer cells via down-regulation of IDO expression which leads to re-activation of immune cell function. We provide a conceptual foundation for the clinical development of 1-MT as an anti-tumor immunomodulator for chemotherapy resistant ovarian cancer patients.
Bubbles to Chondrites-I. Evaporation and condensation experiments, and formation of chondrules
Authors: Nakano Yuki , Hashimoto Akihiko
Published: September 2020

We propose a simple model of chondrule formation that is supported by our new experiments. With a laser-heating and inert-gas-cooling technique, we obtained evaporation and condensation pathways starting with chondritic compositions till ends, and extracted ‘relative volatilities’ of elements from them. Above boiling points, we observed numerous silicate droplets being ejected from collapsed cavities of vapor bubbles on the surface of molten sample, known as jet-droplets. We postulate jet-droplets as origin of chondrules. The formation mechanism of jet-droplets requires a dense and large solid body (>3 cm across), named ‘duston’, for chondrule precursors. Our chondrule formation model presumes dustons having CI-like composition. Upon boiling, a duston ejects jet-droplets from its molten surface and simultaneously forms an adiabatically expanding vapor cloud around it. The jet-droplets supercool and incorporate the supersaturated vapor and fine condensates while they travel through the cloud, thus completing their makeup as chondrules. The compositions and the mixing ratio of the three components (jet-droplet, vapor and condensate) can be exactly predicted by using relative volatilities of elements, given the chondrule composition to be fitted and the conditions: vaporization degree (VD) and redox state (fs) of the duston. We attempt to reproduce bulk compositions of chondrules in total of 600. About 75% chondrules are successfully matched with specific combinations of VD and fs for each chondrule. The model altogether explains 3.5 features of chondrules: maximum size and size-frequency distribution; chemical variety; and textural variety.
Optimization of a Bayesian penalized likelihood algorithm (Q.Clear) for 18F-NaF bone PET/CT images acquired over shorter durations using a custom-designed phantom
Authors: Yoshii Tokiya , Miwa Kenta , Yamaguchi Masashi , Shimada Kai , Wagatsuma Kei , Yamao Tensho , Kamitaka Yuto , Hiratsuka Seiya , Kobayashi Rinya , Ichikawa Hajime , Miyaji Noriaki , Miyazaki Tsuyoshi , Ishii Kenji
Published: September 2020

Background

The Bayesian penalized likelihood (BPL) algorithm Q.Clear (GE Healthcare) allows fully convergent iterative reconstruction that results in better image quality and quantitative accuracy, while limiting image noise. The present study aimed to optimize BPL reconstruction parameters for18F-NaF PET/CT images and to determine the feasibility of18F-NaF PET/CT image acquisition over shorter durations in clinical practice.

Methods

A custom-designed thoracic spine phantom consisting of several inserts, soft tissue, normal spine, and metastatic bone tumor, was scanned using a Discovery MI PET/CT scanner (GE Healthcare). The phantom allows optional adjustment of activity distribution, tumor size, and attenuation. We reconstructed PET images using OSEM + PSF + TOF (2 iterations, 17 subsets, and a 4-mm Gaussian filter), BPL + TOF (β = 200 to 700), and scan durations of 30–120 s. Signal-to-noise ratios (SNR), contrast, and coefficients of variance (CV) as image quality indicators were calculated, whereas the quantitative measures were recovery coefficients (RC) and RC linearity over a range of activity. We retrospectively analyzed images from five persons without bone metastases (male,n= 1; female,n= 4), then standardized uptake values (SUV), CV, and SNR at the 4th, 5th, and 6th thoracic vertebra were calculated in BPL + TOF (β = 400) images.

Results

The optimal reconstruction parameter of the BPL was β = 400 when images were acquired at 120 s/bed. At 90 s/bed, the BPL with a β value of 400 yielded 24% and 18% higher SNR and contrast, respectively, than OSEM (2 iterations; 120 s acquisitions). The BPL was superior to OSEM in terms of RC and the RC linearity over a range of activity, regardless of scan duration. The SUVmaxwere lower in BPL, than in OSEM. The CV and vertebral SNR in BPL were superior to those in OSEM.

Conclusions

The optimal reconstruction parameters of18F-NaF PET/CT images acquired over different durations were determined. The BPL can reduce PET acquisition to 90 s/bed in18F-NaF PET/CT imaging. Our results suggest that BPL (β = 400) on SiPM-based TOF PET/CT scanner maintained high image quality and quantitative accuracy even for shorter acquisition durations.
Housing wealth inequality in urban China: the transition from welfare allocation to market differentiation
Authors: Li Chunling , Fan Yiming
Published: September 2020

The differentiation of housing assets is an important embodiment of wealth inequality and is also an important dimension of social stratification. The housing distribution in China has experienced a transition from welfare allocation to market distribution over the decades. This process has led to a change in the housing stratification mechanism and widened housing wealth inequality, which has evoked theoretical disputes about “market transition,” “power persistence,” and “power derivation.” Based on the 2017 Chinese Social Survey (CSS), this article examines the housing wealth inequality in urban China and probes the major drivers of housing stratification. The results suggest that with the progress of housing marketization, market mechanisms have replaced the original socialist redistribution mechanisms and have become the major drivers of housing wealth inequality. However, some of the original socialist institutional arrangements continue to have strong effects on housing wealth inequality. The persisting institutional effect may provide a new perspective for exploring housing wealth inequality in contemporary urban China.
Mechanical, chemical, structural analysis and comparative release of PDGF-AA from L-PRF, A-PRF and T-PRF - an in vitro study
Authors: Ravi Shravanthy , Santhanakrishnan Muthukumar
Published: September 2020

Background

Platelet concentrates have been popularly used in regenerative periodontal therapy as they are autologous in origin and they provide a supernatural concentration of platelets, growth factors and leukocytes. The release profile of various growth factors is considered important during the various phases of wound healing with the most important being the inflammatory phase where the release of the growth factors help in recruitment of cells and in collagen production. With the more recent modifications of PRF namely A-PRF and T-PRF, the mechanical and chemical degradation properties have also improved. The aim of the present study was to correlate the release profile of PDGF-AA from various forms of platelet concentrates (L-PRF, A-PRF, T-PRF) based on their mechanical and chemical properties.

Methods

Blood samples were drawn from 2 male and 3 female systemically healthy patients between 20 and 25 years of age who were about to undergo periodontal regeneration for PRF preparation. The blood sample was immediately centrifuged using a table top centrifuge (Remi R4C) at 1060 rpm (208 x g) for 14 min for A-PRF preparation, 1960 rpm (708 x g) for 12 min for L-PRF preparation and 1960 rpm (708 x g) for 12 min in titanium tubes for T-PRF preparation. Tensile test was performed using universal testing machine. The in vitro degradation test of the prepared PRF membranes were conducted by placing the PRF membrane in 10 ml of pH 7.4 PBS on an orbital shaker set at 50 rpm. SEM evaluation of the PRF membrane was done under both low and high magnification. In order to determine the amount of released growth factor PDGF-AA at 15 min, 60 min, 8 h, 1 day, 3 days, and 10 days, samples were placed into a shaking incubator at 37 °C to allow for growth factor release into the culture media.

Results

On comparing the three PRF membranes, it was found that T-PRF contained the maximum tensile strength (404.61 ± 5.92 MPa) and modulus of elasticity (151.9 ± 6.92 MPa). Statistically significant differences between the three groups were found on comparing the groups for their mechanical properties. In the degradation test, it was found that the maximum amount of degradation was found in L-PRF (85.75%), followed by A-PRF (84.18%) and the least was found in T-PRF (82.27%). T-PRF released the highest amount of PDGF-AA (6060.4 pg/ml) at early time points when compared to A-PRF (5935.3 pg/ml). While T-PRF had rapid release of PDGF-AA, A-PRF had a sustained release of growth factors released at later time points.

Conclusion

Results from the present study indicate that A-PRF is the most favourable form of platelet concentrate in regenerative periodontal therapy as it has a sustained release of growth factors over time.
Partnering with frail or seriously ill patients in research: a systematic review
Authors: Ludwig Claire , Graham Ian D. , Gifford Wendy , Lavoie Josee , Stacey Dawn
Published: September 2020

Background

The expectation to include patients as partners in research has steadily gained momentum. The vulnerability of frail and/or seriously ill patients provides additional complexity and may deter researchers from welcoming individuals from this patient population onto their teams. The aim was to synthesize the evidence on the engagement of frail and/or seriously ill patients as research partners across the research cycle.

Methods

A systematic review was conducted using PRISMA guidelines. A search strategy included MEDLINE®, EMBASE®, Cumulative Index to Nursing and Allied Health Literature (CINAHL), and PsycINFO from database inception to April, 2019. Eligible studies were peer-reviewed qualitative, quantitative, and mixed methods research reporting on the engagement of frail and/or seriously ill patients as partners on research teams. The Mixed Methods Appraisal Tool was used to appraise study quality. Narrative analysis was conducted.

Results

Of 8763 citations, 30 were included. Most studies included individuals with cancer on the research team (60%). Barriers included: lack of time and resources (50%), discontinuity in contribution (37%), and concerns for well-being (33%). Facilitators included: trust and mutual respect (60%), structural accessibility (57%), flexibility in timing and methods of engagement (43%), and attention to care and comfort, (33%). Perceived impacts for patients included: renewed personal sense of agency (37%) and emotional/peer support (37%). Impacts for researchers included sensitization to the lived experience of disease (57%) and an increased appreciation of the benefits of patient engagement (23%). Research design, execution, and outcomes, developed with patients, were deemed more suitable, relevant and reflective of patients’ priorities.

Conclusions

There is emerging evidence to suggest that research partnerships with frail and/or seriously ill patients can be achieved successfully. Patients mostly report benefit from partnering with research teams. Frailty and/or serious illness do present legitimate concerns for their well-being but appear to be successfully mitigated when researchers ensure that the purpose of engagement is well-defined, the timing and methods of engagement are flexible, and the practical and emotional needs of patient partners are addressed throughout the process.

Systematic review registration

The systematic review protocol was registered with the International Prospective Register of Systematic Reviews PROSPERO (CRD42019127994).
“‘You lose your hair, what’s the big deal?’ I was so embarrassed, I was so self-conscious, I was so depressed:” a qualitative interview study to understand the psychosocial burden of alopecia areata
Authors: Aldhouse Natalie V. J. , Kitchen Helen , Knight Sarah , Macey Jake , Nunes Fabio P. , Dutronc Yves , Mesinkovska Natasha , Ko Justin M. , King Brett A. , Wyrwich Kathleen W.
Published: September 2020

Background

Alopecia areata (AA) is characterized by hair loss that can affect the scalp and body. This study describes the psychosocial burden of AA.

Methods

Participants diagnosed with AA who had experienced ≥50% scalp hair loss according to the Severity of Alopecia Tool (SALT) were identified by clinicians. A semi-structured interview guide, developed with expert clinician input, included open-ended questions to explore patients’ experiences of living with AA. Data were thematically analyzed to identify concepts and relationships.

Results

Participants (n = 45, 58% female, mean age 33.3 years [range 15–72], mean SALT 67.2 [range 0–100]) described the AA diagnosis as “devastating”. Both males and females reported emotional and psychological impacts of AA including feeling sad/depressed (n = 21), embarrassed/ashamed (n = 10) and angry/frustrated (n = 3). Patients felt helpless (n = 5) due to the unpredictability of disease recurrence, and anxious (n = 19) about judgement from others. Many patients avoided social situations (n = 18), which impaired relationships and increased isolation. Coping strategies included concealment of hair loss through wigs or make-up, although fear of the displacement of these coverings also caused anxiety and the avoidance of activities that could result in scalp exposure (n = 22). Some patients became more accepting of AA over time, which lessened the emotional impact, though efficacious treatment was still desired. A conceptual framework was developed, and a conceptual model was created to depict the relationship between the physical signs/symptoms and the associated psychosocial effects of AA.

Conclusion

AA impairs patients’ emotional and psychological wellbeing, relationships and lifestyles. Greater disease awareness and effective treatments are needed.
Fusarium wilt biocontrol and tomato growth stimulation, using endophytic bacteria naturally associated with Solanum sodomaeum and S. bonariense plants
Authors: Aydi-Ben-Abdallah Rania , Jabnoun-Khiareddine Hayfa , Daami-Remadi Mejda
Published: September 2020

Background

Fusarium wilt biocontrol using endophytic microorganisms may represent a potentially attractive and environmentally safe alternative since endophytes could better limit disease incidence and severity through inhibition of the systemic fungus progress.

Main body of the abstract

Twenty-three endophytic bacterial isolates, naturally associated with Solanum sodomaeum and Solanum bonariense, were evaluated for their ability to control Fusarium wilt of tomato induced by Fusarium oxysporum f. sp. lycopersici (FOL) and to promote plant growth. Selected endophytic isolates were screened in vivo, using the root dipping and the culture substrate drenching methods. The most bioactive isolates were subjected to morphological and biochemical characterization and subsequent identification through 16S rDNA sequencing genes. Seven isolates (Stenotrophomonas maltophilia S23, S24, S26 and S28; Bacillus sp. SV81; Azotobacter chroococcum S11; and Serratia marcescens S14) were found to be the most efficient in reducing disease severity by 82–96% over control. Treatments with these isolates led to a significant enhancement in growth parameters, estimated at 45.5–61 and 24.2–70.5% than the control, in tomato plants infected or not with FOL, respectively. Diffusible and volatile metabolites released from bacterial cultures had significantly limited FOL radial growth. All isolates were positive for indole-3-acetic acid (IAA) production. S. marcescens S14, S. maltophilia S28, and Bacillus sp. SV81 exhibited a positive phosphate solubilization activity. Production of chitinase, protease, pectinase, and hydrogen cyanide were also investigated.

Short conclusion

This study clearly demonstrated that endophytic bacteria recovered from these 2 Solanum species can be explored as promising biocontrol agents active against FOL and are able to enhance tomato growth.
Magnetic resonance venography for 3-dimensional live guidance during venous sinus stenting
Authors: Kishore Vaishnavi , Sundararajan Sri Hari , Doustaly Raphael , Michael Marissa , Xuan Dwight , Link Thomas , Rapoport Benjamin , Patsalides Athos
Published: September 2020

Purpose

The purpose of this study was to report the technique for intraprocedural guidance of endovascular Venous Sinus Stenting procedures using 3-Dimensional (3D) Magnetic Resonance Venography (MRV) as an overlay on live biplanar fluoroscopy.

Materials and methods

Venous sinus stenting procedures performed between April and December, 2017 with 3D MRV fusion for live guidance were reviewed in this study. A thin-slice, contrast-enhanced MR Venogram was used to create 2 3D models – vessels and skull – for procedural guidance via augmented fluoroscopy (Vessel ASSIST, GE Healthcare, Chicago, IL). The skull model was used in the registration of the 3D overlay on both the frontal and lateral planes, which required 1–2 min of procedural time. The vessel model was used to mark landmarks such as the cortical vein ostia and stenosis on the 3D overlay fused with biplanar fluoroscopy.

The retrospective imaging review was conducted by 3 neurointerventionalists and relied on a consensus confidence ranking on a 3-point Likert scale from 1- low confidence to 3- high confidence. The neurointerventionalists first reviewed the conventional 2-dimensional pre-stent deployment fluoroscopy images and then reviewed the corresponding images with the 3D MRV overlay. They ranked their confidence in their understanding of cortical venous anatomy for each group. Statistical analysis was performed using a Paired T Test at a 99% confidence interval.

Results

Ten cases were included in the retrospective image review. Operator confidence regarding the location of cortical veins was significantly increased using 3D MRV fusion during venous sinus stenting procedures (1.9 vs 2.9, p = .001).

Conclusion

3-Dimensional MRV fusion is feasible and helpful in understanding the venous sinus anatomy and location of important cortical veins during venous sinus stenting procedures.
A single nucleotide polymorphism assay sheds light on the extent and distribution of genetic diversity, population structure and functional basis of key traits in cultivated north American cannabis
Authors: Henry Philippe , Khatodia Surender , Kapoor Karan , Gonzales Britni , Middleton Alexis , Hong Kevin , Hilyard Aaron , Johnson Steve , Allen Davis , Chester Zachary , Jin Dan , Rodriguez Jule José Carlos , Wilson Iain , Gangola Manu , Broome Jason , Caplan Deron , Adhikary Dinesh , Deyholos Michael K. , Morgan Michael , Hall Oliver W. , Guppy Brent J. , Orser Cindy
Published: September 2020

Background

The taxonomic classification of Cannabis genus has been delineated through three main types: sativa (tall and less branched plant with long and narrow leaves), indica (short and highly branched plant with broader leaves) and ruderalis (heirloom type with short stature, less branching and small thick leaves). While still under discussion, particularly whether the genus is polytypic or monotypic, this broad classification reflects putative geographical origins of each group and putative chemotype and pharmacologic effect.

Methods

Here we describe a thorough investigation of cannabis accessions using a set of 23 highly informative and polymorphic SNP (Single Nucleotide Polymorphism) markers associated with important traits such as cannabinoid and terpenoid expression as well as fibre and resin production. The assay offers insight into cannabis population structure, phylogenetic relationship, population genetics and correlation to secondary metabolite concentrations. We demonstrate the utility of the assay for rapid, repeatable and cost-efficient genotyping of commercial and industrial cannabis accessions for use in product traceability, breeding programs, regulatory compliance and consumer education.

Results

We identified 5 clusters in the sample set, including industrial hemp (K5) and resin hemp, which likely underwent a bottleneck to stabilize cannabidiolic acid (CBDA) accumulation (K2, Type II & III). Tetrahydrocannabinolic acid (THCA) resin (Type I) makes up the other three clusters with terpinolene (K4 - colloquial “sativa” or “Narrow Leaflet Drug” (NLD), myrcene/pinene (K1) and myrcene/limonene/linalool (K3 - colloquial “indica”, “Broad Leaflet Drug” (BLD), which also putatively harbour an active version of the cannabichrometic acid Synthase gene (CBCAS).

Conclusion

The final chemical compositions of cannabis products have key traits related to their genetic identities. Our analyses in the context of the NCBI Cannabis sativa Annotation Release 100 allows for hypothesis testing with regards to secondary metabolite production. Genetic markers related to secondary metabolite production will be important in many sectors of the cannabis marketplace. For example, markers related to THC production will be important for adaptable and compliant large-scale seed production under the new US Domestic Hemp Production Program.
A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development
Authors: LIU Cuixia , LI Zhifang , DOU Lingling , YUAN Yi , ZOU Changsong , SHANG Haihong , CUI Langjun , XIAO Guanghui
Published: September 2020

Background

Cotton is the world’s largest and most important source of renewable natural fiber. BEL1-like homeodomain (BLH) genes are ubiquitous in plants and have been reported to contribute to plant development. However, there is no comprehensive characterization of this gene family in cotton. In this study, 32, 16, and 18 BLH genes were identified from the G. hirsutum, G. arboreum, and G. raimondii genome, respectively. In addition, we also studied the phylogenetic relationships, chromosomal location, gene structure, and gene expression patterns of the BLH genes.

Results

The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic analysis. Among them, 25 members were assigned to 15 chromosomes. Furthermore, gene structure, chromosomal location, conserved motifs, and expression level of BLH genes were investigated in G. hirsutum. Expression profiles analysis showed that four genes (GhBLH1_3, GhBLH1_4, GhBLH1_5, and GhBLH1_6) from BLH1 subfamily were highly expressed during the fiber cell elongation period. The expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid, but not auxin. Exogenous application of gibberellic acid significantly enhanced GhBLH1_3, GhBLH1_4, and GhBLH1_5 transcripts. Expression levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid treatment.

Conclusions

The BLH gene family plays a very important role in many biological processes during plant growth and development. This study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.
Spatiotemporal perspectives on urban energy transitions: a comparative study of three cities in China
Authors: Castán Broto Vanesa , Mah Daphne , Zhang Fangzhu , Huang Ping , Lo Kevin , Westman Linda
Published: September 2020

This paper develops an integrated framework to study the socio-spatial and temporal dimensions of urban energy transitions to investigate the development and spread of solar energy technologies in urban China. A comparative analysis of three case studies of solar energy transitions in the cities of Foshan (in Guangdong), Rizhao (in Shandong), and Wuxi (in Jiangsu) demonstrates the framework’s applicability. The results map each city’s trajectory towards low carbon energy. Transitions result from dynamic interactions among central and local governments, solar manufacturers, solar installers, and residents. Alongside industrial strategies, locally-specific factors have a determining influence on the eventual outcomes.
The role of neurohormonal blockers in the primary prevention of acute-, early-, and late-onset anthracycline-induced cardiotoxicity
Authors: Ayuna Ahmed , Abidin Nik
Published: September 2020

Background

Anthracycline-induced cardiotoxicity has been classified based on its onset into acute, early, and late. It may have a significant burden on the quality and quantity of life of those exposed to this class of medication. Currently, there are several ongoing debates on the role of different measures in the primary prevention of cardiotoxicity in cancer survivors. Our article aims to focus on the role of neurohormonal blockers in the primary prevention of anthracycline-induced cardiotoxicity, whether it is acute, early, or late onset.

Main body of the abstract

PubMed and Google Scholar database were searched for the relevant articles; we reviewed and appraised 15 RCTs, and we found that angiotensin-converting enzyme inhibitors (ACEI) and B-blockers were the most commonly used agents. Angiotensin II receptor blockers (ARBs) and mineralocorticoid receptor antagonists (MRAs) were used in a few other trials. The follow-up period was on the range of 1–156 weeks (mode 26 weeks). Left ventricular ejection fraction (LVEF), left ventricular diameters, and diastolic function were assessed by either echocardiogram or occasionally by cardiac magnetic resonance imaging (MRI). The occurrence of myocardial injury was assessed by troponin I. It was obvious that neurohormonal blockers reduced the occurrence of LVEF and myocardial injury in 14/15 RCTs.

Short conclusion

Beta-blockers, especially carvedilol and ACEI, especially enalapril, should be considered for the primary prevention of acute- and early-onset cardiotoxicity. ARB and MRA are suitable alternatives when patients are intolerant to ACE-I and B-blockers. We recommend further studies to explore and establish the role of neurohormonal blockers in the primary prevention of the acute-, early-, and late-onset cardiotoxicity.
Uniportal video-assisted thoracoscopic drainless sympathectomy for palmar and palmo-plantar hyperhidrosis: an institutional experience
Authors: El-Hag-Aly Mohammed Ahmed , Soliman Rafik Fekry , Zaid Nehad Abdo , Eldesouky Mahmoud Said , Bakoush Mohamed Mordy , Hagag Mohamed Gamal
Published: September 2020

Background

Primary hyperhidrosis (PH) still impacts negatively the patient’s quality of life (QoL). Progressively, it leads to poorer QoL regardless of gender. The endoscopic thoracic sympathectomy has been used safely and effectively for control of palmar hyperhidrosis, but it is still questionable in palmo-plantar type. We assessed the benefits of uniportal drainless thoracoscopic sympathectomy (UDTS) for palmo-plantar hyperhidrosis patients compared to palmar ones.

This prospective study comprised 213 consecutive patients with PH. They underwent bilateral simultaneous UDTS. We used the Hyperhidrosis Disease Severity Scale (HDSS) scoring system for assessment of QoL pre- and postoperatively. Follow-up was continued for 1-year that was aiming to detect the QoL improvement, recurrence, and compensatory hyperhidrosis (CH).

Results

All patients experienced immediate complete resolution of hyperhidrosis postoperatively. Recurrence was encountered in one patient during the first 6 months. All patients had improved QoL postoperatively, but at the end of 1-year follow-up, 2.8% of patients were still suffering moderate to severe impaired QoL. Mean hospital stay was 31.79 ± 17.5 h. We found significant longer hospital stay in palmar group than palmo-plantar group. There was no significant difference between both groups in neither operative time, recurrence, nor CH.

Conclusions

UDTS offers better QoL for patients with PH even the palmo-plantar type. The palmo-plantar type benefits similarly to the palmar type in terms of comparable postoperative QoL and CH incidence.
User Perceptions of an Electronic Maternal Alerting System
Authors: Klumpner Thomas T. , Kountanis Joanna A. , Bauer Melissa E. , Carver Alissa , Piehl Anne Marie , Smith Roger , Mentz Graciela , Tremper Kevin K.
Published: September 2020

This survey study evaluates the user experience of an electronic maternal early warning system that generates automated pages. Survey domains included the system’s effect on patient care, alarm fatigue, and continued use of the system. The response rate was 47.7% (273 of 572). A majority, 83%, felt that the system should remain in use, and 64.5% felt it improved patient safety. Of those who believed that they had received a page, 51.4% felt that they received pages “too frequently.” Although alarm fatigue was not fully evaluated, providers on our unit support the continued use of this automated maternal electronic surveillance system.
“Whip from the hip”: thigh angular motion, ground contact mechanics, and running speed
Authors: Clark Kenneth P. , Meng Christopher R. , Stearne David J.
Published: September 2020

During high-speed running, lower limb vertical velocity at touchdown has been cited as a critical factor needed to generate large vertical forces. Additionally, greater leg angular velocity has also been correlated with increased running speeds. However, the association between these factors has not been comprehensively investigated across faster running speeds. Therefore, this investigation aimed to evaluate the relationship between running speed, thigh angular motion, and vertical force determinants. It was hypothesized that thigh angular velocity would demonstrate a positive linear relationship with both running speed and lower limb vertical velocity at touchdown. A total of 40 subjects (20 males, 20 females) from various athletic backgrounds volunteered and completed 40 m running trials across a range of sub-maximal and maximal running speeds during one test session. Linear and angular kinematic data were collected from 31–39 m. The results supported the hypotheses, as across all subjects and trials (range of speeds: 3.1–10.0 m s−1), measures of thigh angular velocity demonstrated a strong positive linear correlation to speed (all R2>0.70, p<0.0001) and lower limb vertical velocity at touchdown (all R2=0.75, p<0.0001). These findings suggest thigh angular velocity is strongly related to running speed and lower limb impact kinematics associated with vertical force application.
Quantifying Drosophila adults with the use of a smartphone
Authors: Karpova E. K. , Komyshev E. G. , Genaev M. A. , Adonyeva N. V. , Afonnikov D. A. , Eremina M. A. , Gruntenko N. E.
Published: September 2020

A method for automation of imago quantifying and fecundity assessment in Drosophila with the use of mobile devices running Android operating system is proposed. The traditional manual method of counting the progeny takes a long time and limits the opportunity of making large-scale experiments. Thus, the development of computerized methods that would allow us to automatically make a quantitative estimate of D. melanogaster fecundity is an urgent requirement. We offer a modification of the mobile application SeedCounter that analyzes images of objects placed on a standard sheet of paper for an automatic calculation of D. melanogaster offspring or quantification of adult flies in any other kind of experiment. The relative average error in estimates of the number of flies by mobile app is about 2% in comparison with the manual counting and the processing time is 6 times shorter. Study of the effects of imaging conditions on accuracy of flies counting showed that lighting conditions do not significantly affect this parameter, and higher accuracy can be achieved using high-resolution smartphone cameras (8 Mpx and more). These results indicate the high accuracy and efficiency of the method suggested.
Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development
Authors: Chu Daniel , Nguyen An , Smith Spenser S. , Vavrušová Zuzana , Schneider Richard A.
Published: September 2020

Precisely altering gene expression is critical for understanding molecular processes of embryogenesis. Although some tools exist for transgene misexpression in developing chick embryos, we have refined and advanced them by simplifying and optimizing constructs for spatiotemporal control. To maintain expression over the entire course of embryonic development we use an enhanced piggyBac transposon system that efficiently integrates sequences into the host genome. We also incorporate a DNA targeting sequence to direct plasmid translocation into the nucleus and a D4Z4 insulator sequence to prevent epigenetic silencing. We designed these constructs to minimize their size and maximize cellular uptake, and to simplify usage by placing all of the integrating sequences on a single plasmid. Following electroporation of stage HH8.5 embryos, our tetracycline-inducible promoter construct produces robust transgene expression in the presence of doxycycline at any point during embryonic development in ovo or in culture. Moreover, expression levels can be modulated by titrating doxycycline concentrations and spatial control can be achieved using beads or gels. Thus, we have generated a novel, sensitive, tunable, and stable inducible-promoter system for high-resolution gene manipulation in vivo.
Establishing the pattern of the vertebrate limb
Authors: McQueen Caitlin , Towers Matthew
Published: September 2020

The vertebrate limb continues to serve as an influential model of growth, morphogenesis and pattern formation. With this Review, we aim to give an up-to-date picture of how a population of undifferentiated cells develops into the complex pattern of the limb. Focussing largely on mouse and chick studies, we concentrate on the positioning of the limbs, the formation of the limb bud, the establishment of the principal limb axes, the specification of pattern, the integration of pattern formation with growth and the determination of digit number. We also discuss the important, but little understood, topic of how gene expression is interpreted into morphology.
Gonads or body? Differences in gonadal and somatic photoperiodic growth response in two vole species
Authors: van Rosmalen Laura , van Dalum Jayme , Hazlerigg David G. , Hut Roelof A.
Published: September 2020

To optimally time reproduction, seasonal mammals use a photoperiodic neuroendocrine system (PNES) that measures photoperiod and subsequently drives reproduction. To adapt to late spring arrival at northern latitudes, a lower photoperiodic sensitivity and therefore a higher critical photoperiod for reproductive onset is necessary in northern species to arrest reproductive development until spring onset. Temperature-photoperiod relationships, and hence food availability-photoperiod relationships, are highly latitude dependent. Therefore, we predict PNES sensitivity characteristics to be latitude-dependent. Here, we investigated photoperiodic responses at different times during development in northern (tundra/root vole, Microtus oeconomus) and southern vole species (common vole, Microtus arvalis) exposed to constant short (SP) or long photoperiod (LP). Although, the tundra vole grows faster under LP, no photoperiodic effect on somatic growth is observed in the common vole. Contrastingly, gonadal growth is more sensitive to photoperiod in the common vole, suggesting that photoperiodic responses in somatic and gonadal growth can be plastic, and might be regulated through different mechanisms. In both species, thyroid-stimulating-hormone-β subunit (Tshβ) and iodothyronine- deiodinase 2 (Dio2) expression is highly increased under LP, whereas Tshr and Dio3 decreases under LP. High Tshr levels in voles raised under SP may lead to increased sensitivity to increasing photoperiods later in life. The higher photoperiodic induced Tshr response in tundra voles suggests that the northern vole species might be more sensitive to TSH when raised under SP. In conclusion, species differences in developmental programming of the PNES, which is dependent on photoperiod early in development, may form different breeding strategies evolving as part of latitudinal adaptation.
Intrafractional fiducial marker position variations in stereotactic liver radiotherapy during voluntary deep inspiration breath-hold
Authors: Stick Line Bjerregaard , Vogelius Ivan Richter , Risum Signe , Josipovic Mirjana
Published: September 2020

Objectives:

To evaluate intrafractional fiducial marker position variations during stereotactic body radiotherapy (SBRT) in patients treated for liver metastases in visually guided, voluntary deep inspiration breath-hold (DIBH).

Methods:

10 patients with implanted fiducial markers were studied. Respiratory coaching with visual guidance was used to ensure comfortable voluntary breath-holds for SBRT imaging and delivery. Three DIBH CTs were acquired for treatment planning. Pre- and post-treatment CBCTs were acquired for each of the three treatment fractions. Per-fraction marker position was evaluated on planar 2D kV images acquired during treatment fractions for 4 of the 10 patients.

Results:

The median difference in marker position was 0.3 cm (range, 0.0–0.9 cm) between the three DIBH CTs and 0.3 cm (range, 0.1 to 1.4 cm) between pre- and post-treatment CBCTs. The maximum intrafractional variation in marker position in craniocaudal (CC) direction on planar kV images was 0.7 to 1.3 cm and up to 1.0 cm during a single DIBH.

Conclusion:

Difference in marker position of up to 1.0 cm was observed during a single DIBH despite use of narrow external gating window and visual feedback. Stability examination on pre-treatment DIBH CTs was not sufficient to guarantee per-fraction stability. Evaluation of differences in marker position on pre- and post-treatment CBCT did not always reveal the full magnitude of the intrafractional variation.

Advances in knowledge

To increase treatment accuracy, it is necessary to apply real-time monitoring of the tumour or a reliable internal surrogate when delivering liver SBRT in voluntary DIBH.
Chemical shifts-based similarity restraints improve accuracy of RNA structures determined via NMR
Authors: Lawrence Chad , Grishaev Alexander
Published: September 2020

Determination of structure of RNA via NMR is complicated in large part by the lack of a precise parameterization linking the observed chemical shifts to the underlying geometric parameters. In contrast to proteins, where numerous high-resolution crystal structures serve as coordinate templates for this mapping, such models are rarely available for smaller oligonucleotides accessible via NMR, or they exhibit crystal packing and counter-ion binding artifacts that prevent their use for the chemical shifts analysis. On the other hand, NMR-determined structures of RNA often are not solved at the density of restraints required to precisely define the variable degrees of freedom. In this study we sidestep the problems of direct parameterization of the RNA chemical shifts/structure relationship and examine the effects of imposing local fragmental coordinate similarity restraints based on similarities of the experimental secondary ribose13C/1H chemical shifts instead. The effect of such chemical shift similarity (CSS) restraints on the structural accuracy is assessed via residual dipolar coupling (RDC)-based cross-validation. Improvements in the coordinate accuracy are observed for all of the six RNA constructs considered here as test cases, which argues for routine inclusion of these terms during NMR-based oligonucleotide structure determination. Such accuracy improvements are expected to facilitate derivation of the chemical shift/structure relationships for RNA.
ACE inhibitor, captopril, attenuates cytopathic effects of herpes simplex virus 1 in SH-SY5Y cells
Authors: Wofford Amina S. , Standiford Adam , Harris Kennedy , Salam Ahmad B. , Jackson Cynthia A. , Bradford Chastity N. , Griffin Gerald D.
Published: September 2020

Background: Over 60% of the United States population is infected with herpes simplex virus 1 (HSV-1). Current HSV-1 treatment regimens exert their antiviral effects through a common mechanism of action and suffer from high dosing frequencies, which may contribute to patient noncompliance and the subsequent development of antiviral resistance. Although primarily known for their functions in maintaining homeostatic control of arterial blood and osmotic pressures, components of the Renin-Angiotensin Aldosterone System (RAAS) have been implicated in viral replication and some components demonstrated antiviral properties. However, the antiviral properties of RAAS components have not been well characterized in reference to HSV-1.

Methods: To address this gap in knowledge, we evaluated the antiviral effects of captopril, an Angiotensin Converting Enzyme 1 (ACE-1) inhibitor, on HSV-1 infection in SH-SY5Y neuroblastoma cells. We demonstrated that captopril attenuates HSV-1-induced cytopathic effects (CPE) via cell-based and morphological assays. To investigate the potential mechanism, we conducted molecular modeling studies and identified the ability of captopril to interact with and bind HSV-1 glycoprotein D. To determine where in the virus life cycle captopril exerts its protective effects, we performed experiments observing the effect of captopril on both viral entry and replication utilizing a green fluorescent protein-tagged virus and subsequent quantitative Polymerase Chain Reaction.

Results: Results suggest captopril protects cells from HSV-1-induced CPE through its effect on viral replication by increasing cell viability in infected cells and decreasing virus replication, which we propose is modulated through the decreased expression of ICP0.

Conclusions: Collectively, the results presented here support further evaluation of captopril, and other RAAS components, as a basis for potential novel therapeutic interventions for the treatment of HSV-1, its associated pathologies, and potentially other virus infections.