bluuebunny/crossref_metadata_2025 huggingface dataset:
Automated Cell Selection Using Support Vector Machine for Application to Spectral Nanocytology
Authors: Miao Qin , Derbas Justin , Eid Aya , Subramanian Hariharan , Backman Vadim
Published: January 2016

Partial wave spectroscopy (PWS) enables quantification of the statistical properties of cell structures at the nanoscale, which has been used to identify patients harboring premalignant tumors by interrogating easily accessible sites distant from location of the lesion. Due to its high sensitivity, cells that are well preserved need to be selected from the smear images for further analysis. To date, such cell selection has been done manually. This is time-consuming, is labor-intensive, is vulnerable to bias, and has considerable inter- and intraoperator variability. In this study, we developed a classification scheme to identify and remove the corrupted cells or debris that are of no diagnostic value from raw smear images. The slide of smear sample is digitized by acquiring and stitching low-magnification transmission. Objects are then extracted from these images through segmentation algorithms. A training-set is created by manually classifying objects as suitable or unsuitable. A feature-set is created by quantifying a large number of features for each object. The training-set and feature-set are used to train a selection algorithm using Support Vector Machine (SVM) classifiers. We show that the selection algorithm achieves an error rate of 93% with a sensitivity of 95%.
Critical Role of FoxO1 in Granulosa Cell Apoptosis Caused by Oxidative Stress and Protective Effects of Grape Seed Procyanidin B2
Authors: Zhang Jia-Qing , Gao Bin-Wen , Wang Jing , Ren Qiao-Ling , Chen Jun-Feng , Ma Qiang , Zhang Zi-Jing , Xing Bao-Song
Published: January 2016

Reactive oxygen species (ROS) are closely related to the follicular granulosa cell apoptosis. Grape seed procyanidin B2 (GSPB2) has been reported to possess potent antioxidant activity. However, the GSPB2‐mediated protective effects and the underlying molecular mechanisms in granulosa cell apoptosis process remain unknown. In this study, we showed for the first time that GSPB2 treatment decreased FoxO1 protein level, improved granulosa cell viability, upregulated LC3‐II protein level, and reduced granulosa cell apoptosis rate. Under a condition of oxidative stress, GSPB2 reversed FoxO1 nuclear localization and increased its level in cytoplasm. In addition, FoxO1 knockdown inhibited the protective effects of GSPB2 induced. Our findings suggest that FoxO1 plays a pivotal role in regulating autophagy in granulosa cells, GSPB2 exerts a potent and beneficial role in reducing granulosa cell apoptosis and inducing autophagy process, and targeting FoxO1 could be significant in fighting against oxidative stress‐reduced female reproductive system diseases.
The Prevalence of Headache in Crohn’s Disease: Single-Center Experience
Authors: Anadol Kelleci Ulker , Calhan Turan , Sahin Abdurrahman , Kahraman Resul , Ozdil Kamil , Sokmen Haci Mehmet , Yalcin Destina
Published: January 2016

Objectives. This study is aimed at studying the prevalence and characteristics of different types of headaches in patients with Crohn’s disease.Materials and Methods. 51 patients in Crohn’s disease group (F/M: 26/25) and 51 patients in control group (F/M: 27/24) were involved. Patients in Crohn’s disease group were diagnosed and monitored according to European Crohn’s and Colitis Organization diagnostic criteria. The control group composed of healthy subjects with similar age and sex to Crohn’s disease group. Headache was classified using the International Headache Society II criteria.Results. Headache was reported by 35/51 (68.6%) patients in Crohn’s disease group and 21/51 (41.2%) patients in the control group. The prevalence of headache was statistically high in the group with Crohn’s disease (OR: 3.125 (95% CI: 1.38–7.04);p=0.01). Comparing two groups with respect to their subtypes of headaches resulted in that the tension-type headache was statistically (p=0.008) higher in Crohn’s disease group (26/51) than in the control group (12/51). However, no significant difference was found in the migraine-type headache (p=1).Conclusions. This study indicates that the prevalence of headache is high in patients with Crohn’s disease and most commonly associated with the tension-type headache.
The Application of Response Surface Methodology in the Investigation of the Tribological Behavior of Palm Cooking Oil Blended in Engine Oil
Authors: Sakinah M. H. , Amirruddin A. K. , Kadirgama K. , Ramasamy D. , Rahman M. M. , Noor M. M.
Published: January 2016

The purpose of this study was to determine the optimal design parameters and to indicate which of the design parameters are statistically significant for obtaining a low coefficient of friction (COF) and low wear rate with waste palm oil blended with SAE 40. The tribology performance was evaluated using a piston-ring-liner contact tester. The design of experiment (DOE) was constructed by using response surface methodology (RSM) to minimize the number of experimental conditions and to develop a mathematical model between the key process parameters such as rotational speeds (200 rpm to 300 rpm), volume concentration (0% to 10% waste oil), and applied loads (2 kg to 9 kg). Analysis of variance (ANOVA) test was also carried out to check the adequacy of the empirical models developed. Scanning electron microscopy (SEM) was used to examine the damage features at the worn surface under lubricant contact conditions.
Measurement of the Red Blood Cell Distribution Width Improves the Risk Prediction in Cardiac Resynchronization Therapy
Authors: Boros András Mihály , Perge Péter , Jenei Zsigmond , Karády Júlia , Zima Endre , Molnár Levente , Becker Dávid , Gellér László , Prohászka Zoltán , Merkely Béla , Széplaki Gábor
Published: January 2016

Objectives. Increases in red blood cell distribution width (RDW) and NT-proBNP (N-terminal pro-B-type natriuretic peptide) predict the mortality of chronic heart failure patients undergoing cardiac resynchronization therapy (CRT). It was hypothesized that RDW is independent of and possibly even superior to NT-proBNP from the aspect of long-term mortality prediction.Design. The blood counts and serum NT-proBNP levels of 134 patients undergoing CRT were measured. Multivariable Cox regression models were applied and reclassification analyses were performed.Results. After separate adjustment to the basic model of left bundle branch block, beta blocker therapy, and serum creatinine, both the RDW > 13.35% and NT-proBNP > 1975 pg/mL predicted the 5-year mortality (n=57). In the final model including all variables, the RDW [HR = 2.49 (1.27–4.86);p=0.008] remained a significant predictor, whereas the NT-proBNP [HR = 1.18 (0.93–3.51);p=0.07] lost its predictive value. On addition of the RDW measurement, a 64% net reclassification improvement and a 3% integrated discrimination improvement were achieved over the NT-proBNP-adjusted basic model.Conclusions. Increased RDW levels accurately predict the long-term mortality of CRT patients independently of NT-proBNP. Reclassification analysis revealed that the RDW improves the risk stratification and could enhance the optimal patient selection for CRT.
Synchronous Renal Cell Carcinoma and Gastrointestinal Malignancies
Authors: Dafashy Tamer J. , Ghaffary Cameron K. , Keyes Kyle T. , Sonstein Joseph
Published: January 2016

While renal cell carcinoma is the most commonly diagnosed neoplasm of the kidney, its simultaneous diagnosis with a gastrointestinal malignancy is a rare, but well reported phenomenon. This discussion focuses on three independent cases in which each patient was diagnosed with renal cell carcinoma and a unique synchronous gastrointestinal malignancy. Case1explores the diagnosis and surgical intervention of a 66-year-old male patient synchronously diagnosed with clear cell renal cell carcinoma and a carcinoid tumor of the small bowel. Case2describes the diagnosis and surgical intervention of a 61-year-old male found to have clear cell renal cell carcinoma and a mucinous appendiceal neoplasm. Lastly, Case3focuses on the interventions and management of a 36-year-old female diagnosed with synchronous clear cell renal carcinoma and hereditary nonpolyposis colorectal cancer. This case series examines each distinct patient’s presentation, discusses the diagnosis, and compares and contrasts the findings while discussing the literature on this topic.
Impaired Functional Connectivity in the Prefrontal Cortex: A Mechanism for Chronic Stress-Induced Neuropsychiatric Disorders
Authors: Negrón-Oyarzo Ignacio , Aboitiz Francisco , Fuentealba Pablo
Published: January 2016

Chronic stress-related psychiatric diseases, such as major depression, posttraumatic stress disorder, and schizophrenia, are characterized by a maladaptive organization of behavioral responses that strongly affect the well-being of patients. Current evidence suggests that a functional impairment of the prefrontal cortex (PFC) is implicated in the pathophysiology of these diseases. Therefore, chronic stress may impair PFC functions required for the adaptive orchestration of behavioral responses. In the present review, we integrate evidence obtained from cognitive neuroscience with neurophysiological research with animal models, to put forward a hypothesis that addresses stress-induced behavioral dysfunctions observed in stress-related neuropsychiatric disorders. We propose that chronic stress impairs mechanisms involved in neuronal functional connectivity in the PFC that are required for the formation of adaptive representations for the execution of adaptive behavioral responses. These considerations could be particularly relevant for understanding the pathophysiology of chronic stress-related neuropsychiatric disorders.
High Prevalence of Human Papillomavirus in Colorectal Cancer in Hispanics: A Case-Control Study
Authors: Bernabe-Dones Raul D. , Gonzalez-Pons Maria , Villar-Prados Alejandro , Lacourt-Ventura Mercedes , Rodríguez-Arroyo Heriberto , Fonseca-Williams Sharon , Velazquez Francisco E. , Diaz-Algorri Yaritza , Lopez-Diaz Sofia M. , Rodríguez Nayra , Yamamura Yasuhiro , Cruz-Correa Marcia
Published: January 2016

The role of Human Papillomavirus (HPV) in colorectal carcinogenesis remains elusive. Based on the high incidence of HPV-associated malignancies among Puerto Rican Hispanics, this study aimed to assess the prevalence of HPV infection and viral integration in colorectal tissues in order to evaluate its putative role in colorectal cancer (CRC). In this case-control study, the prevalence of HPV infection in CRC (casesn= 45) and normal colon mucosa from cancer-free subjects (controlsn= 36) was assessed by a nested PCR strategy. HPV-16 genotyping was performed in HPV-positive tissues and the physical status of the HPV-16 genome was determined by E2 detection. HPV was detected in 19 of 45 (42.2%) CRC cases (mean age 61.1 ± 10.7 years, 24 males) and in 1 of 36 (2.8%) controls (mean age 60.9 ± 9.6 years, 24 males) with an OR = 25.58 (95% CI 3.21 to 203.49). HPV-16 was detected in 63.2% of the HPV-positive colorectal tumors; genome integration was observed in all HPV-16 positive cases. This is the first report showing the high prevalence of HPV infections in Caribbean Hispanic colorectal tumors. Despite evidence of HPV integration into the host genome, further mechanistic analysis examining HPV oncoprotein expression and the putative role of these oncoproteins in colorectal carcinogenesis is warranted.
Singularity Analysis for a Class of Porous Medium Equation with Time-Dependent Coefficients
Authors: Xia Anyin , Pu Xianxiang , Li Shan
Published: January 2016

This paper concerns the singularity and global regularity for the porous medium equation with time-dependent coefficients under homogeneous Dirichlet boundary conditions. Firstly, some global regularity results are established. Furthermore, we investigate the blow-up solution to the boundary value problem. The upper and lower estimates to the lifespan of the singular solution are also obtained here.
The Protective Effects of Isoliquiritigenin and Glycyrrhetinic Acid against Triptolide‐Induced Oxidative Stress in HepG2 Cells Involve Nrf2 Activation
Authors: Cao Ling-Juan , Li Huan-De , Yan Miao , Li Zhi-Hua , Gong Hui , Jiang Pei , Deng Yang , Fang Ping-Fei , Zhang Bi-Kui
Published: January 2016

Triptolide (TP), an active ingredient of Tripterygium wilfordii Hook f., possesses a wide range of biological activities. Oxidative stress likely plays a role in TP‐induced hepatotoxicity. Isoliquiritigenin (ISL) and glycyrrhetinic acid (GA) are potent hepatoprotection agents. The aim of the present study was to investigate whether Nrf2 pathway is associated with the protective effects of ISL and GA against TP‐induced oxidative stress or not. HepG2 cells were treated with TP (50 nM) for 24 h after pretreatment with ISL and GA (5, 10, and 20 μM) for 12 h and 24 h, respectively. The results demonstrated that TP treatment significantly increased ROS levels and decreased GSH levels. Both ISL and GA pretreatment decreased ROS and meanwhile enhanced intracellular GSH content. Additionally, TP treatment obviously decreased the protein expression of Nrf2 and its target genes including HO‐1 and MRP2 except NQO1. Moreover, both ISL and GA displayed activities as inducers of Nrf2 and increased the expression of HO‐1, NQO1, and MRP2. Taken together the current data confirmed that ISL and GA could activate the Nrf2 antioxidant response in HepG2 cells, increasing the expression of its target genes which may be partly associated with their protective effects in TP‐induced oxidative stress.
Temporal and Spatial Variability of Air Temperatures in Estonia during 1756–2014
Authors: Eensaar Agu
Published: January 2016

The change in the statistical and temporal parameters of air temperatures in the Estonian cities, that is, Tallinn and Tartu, was analyzed for two centuries. The results showed that the change of air temperature in Estonia exceeded 0.5°C per ten years for the time 1979–2012. For the longer period, that is, 1880–2012, the average annual rise in the air temperature was within the range of 0.1°C per ten years. The analysis of frequency distributions of the average annual air temperatures and Welch’s t-test demonstrated the considerable rise in air temperature (the significance level of 0.05) in Estonia, which took place in 1901–2014 and was witnessed only in the months from November to April. However, no significant rise in air temperature was detected in Estonia from May to October.
Errors Nature of the Narrowband PLC Transmission in Smart Lighting LV Network
Authors: Kiedrowski Piotr
Published: January 2016

This paper presents characteristics of the Frame Error Rate versus Signal to Noise Ratio obtained from Smart Lighting LV network. These characteristics are the results of carrying out many field-trials in three locations. During the one-year tests period lighting systems evolved from the traditional, gas-discharge not individually dimmable system to LED or gas-discharge Smart Lighting system controlled with the use of PLC technology every lamp individually. A different influence of the frame length on the [Formula: see text] characteristic for the gas-discharge and LED LV networks led to examining the bit errors distribution. The bit errors distribution characteristics of the narrowband PLC transmission over the Smart Metering LV network are also presented in this paper. All the presented data in this work are the results of the long time field-trials, which were verified in the lab conditions.
Modeling Lipase-Catalyzed Biodiesel Production in [BMIM][PF6]
Authors: Yang JianJun , Yang MingYan
Published: January 2016

Lipase-catalyzed biodiesel production models in room temperature ionic liquids (RTILs) reaction medium available in the literature are valid especially for mixing intensity. In this paper, a preliminary model is established in order to try to describe the lipase-catalyzed biodiesel production process in RTILs in a stirring type bioreactor. Mixing intensity and time delay were inspected for the reaction model in [BMIM][PF6] medium. As a result, this model is a good explanation for these actual reaction conditions in RTILs. The model prediction curves well describe the experimental data indicating this bioenzymatic reaction model is effective and reliable in certain conditions.
Workup and Management of Persistent Neuralgia following Nerve Block
Authors: Weyker Paul David , Webb Christopher Allen-John , Pham Thoha M.
Published: January 2016

Neurological injuries following peripheral nerve blocks are a relatively rare yet potentially devastating complication depending on the type of lesion, affected extremity, and duration of symptoms. Medical management continues to be the treatment modality of choice with multimodal nonopioid analgesics as the cornerstone of this therapy. We report the case of a 28-year-old man who developed a clinical common peroneal and lateral sural cutaneous neuropathy following an uncomplicated popliteal sciatic nerve block. Workup with electrodiagnostic studies and magnetic resonance neurography revealed injury to both the femoral and sciatic nerves. Diagnostic studies and potential mechanisms for nerve injury are discussed.
The Chronic and Short-Term Effects of Gefinitib on Airway Remodeling and Inflammation in a Mouse Model of Asthma
Authors: Song Liqiang , Tang Huanzhang , Liu Dapeng , Song Jiao , Wu Yunfu , Qu Shuoyao , Li Yan
Published: January 2016

Background: Asthma is a complex and heterogeneous chronic inflammatory disorder which is characterized by airway remodeling and airway inflammation, including goblet cell and airway smooth muscle cell hyperplasia, mucus hypersecretion and eosinophils infiltration. Epidermal growth factor receptor (EGFR) plays an important role in goblet cell hyperplasia and mucus hypersecretion. We aimed to investigate the effects of gefitinib, an EGFR inhibitor, on ovalbumin (OVA)-induced airway remodeling and inflammation of a mouse model of asthma. Methods: Pathological changes of OVA sensitization of BALB/c mice were measured by H&E and PAS staining; pEGFR, Bcl-2 and Bax expression was measured by western blot; ELISA was used to measure the level of muc5ac, IL-13 and IFN-γ; TUNEL staining was used to detect goblet cell apoptosis. Results: At the present study, H&E and PAS staining showed that mice pretreated with gefinitib developed fewer pathological changes compared with asthmatic mice and gefinitib treatment asthmatic mice, such as a remarkable reduction in airway inflammation, goblet cell and airway smooth muscle cell hyperplasia. Chronic gefitinib treatment or short-term gefitinib treatment significant down-regulate the expression of pEGFR compared with asthma group. Also, chronic gefitinib treatment markedly decreased the levels of muc5ac and IL-13 in BALF, whereas the level of IFN-γ did not change obviously. TUNEL staining showed that the goblet cell apoptosis rate was much higher in the short-term gefinitib treatment group compared with the asthma and chronic gefitinib treatment group which was accompanied by a decrease in Bcl-2 levels and an increase in Bax expression in goblet cells. Conclusion: In summary, our results suggested that gefinitib may have a potential role in airway remodeling and inflammation, and may be an effective pharmacotherapy for asthma.
Atorvastatin Inhibits Myocardial Apoptosis in a Swine Model of Coronary Microembolization by Regulating PTEN/PI3K/Akt Signaling Pathway
Authors: Wang Jiangyou , Chen Han , Zhou You , Su Qiang , Liu Tao , Wang Xian-tao , Li Lang
Published: January 2016

Background/Aims: Phosphatase and tensin homolog deleted on chromosome ten (PTEN) has been recognized as a promoter of apoptosis in various tissues, and revealed to be up-regulated in circumstances of coronary microembolization (CME). However, whether this functional protein could be modified by pretreatment of atorvastatin in models of CME has not been disclosed yet. Methods: Swine CME was induced by intra-coronary injection of inertia plastic microspheres (diameter 42 μm) into left anterior descending coronary, with or without pretreatment of atorvastatin or PTEN siRNA. Echocardiologic measurements, pathologic examination, TUNEL staining and western blotting were applied to assess their functional, morphological and molecular effects in CME. Results: PTEN were aberrantly up-regulated in cardiomyocytes following CME, with both the mRNA and protein levels increased after CME modeling. Pretreatment with atorvastatin could attenuate the induction of PTEN. Furthermore, down-regulation of PTEN in vivo via siRNA was associated with an improved cardiac function, attenuated myocardial apoptosis, and concomitantly inhibited expressions of key proapoptotic proteins such as Bax, cleaved-caspase-3. Interestingly, atorvastatin could markedly attenuate PTEN expression and therefore partially reverse cardiac dysfunction and attenuate the apoptosis of the myocardium following CME. Conclusion: Modulation of PTEN was probably as a potential mechanism involved in the beneficial effects of pretreatment of atorvastatin to cardiac function and apoptosis in large animal models of CME.
All-Trans Retinoic Acid Attenuates Hypoxia-Induced Injury in NRK52E Cells via Inhibiting NF-κB/VEGF and TGF-β2/VEGF Pathway
Authors: Xu Yong , Gao Ai-Mei , Ji Li-Juan , Li Xing , Zhong Li-Li , Li Hai-Lun , Zheng Dong-Hui
Published: January 2016

Background/Aims: Hypoxia has recently been proposed as one of the most important factors in progressive renal injury. Hypoxia-induced vascular endothelial growth factor (VEGF) expression may play a critical role in maintaining peritubular capillary endothelium in renal disease. This study was designed to investigate the effect and underlying mechanism of all-trans retinoic acid (ATRA) on hypoxia-induced injury in NRK52E cells. Methods: For mimicking hypoxia, cells were treated with 100 µM of cobalt chloride (CoCl2). The cell viability, expression of VEGF, p65, transforming growth factor-β2 (TGF-β2) and serine carboxypeptidase 1 (Scpep1), and nuclear factor of kappaB (NF-κB) activities after ATRA treatment were determined by MTT, western blot and electrophoretic mobility shift assay. Co-immunoprecipitation analysis was performed to demonstrate whether Scpep1 interacted with TGF-β2. Results: It was found that CoCl2 triggered hypoxia injury and significantly reduced cell viability. ATRA pretreatment increased the cell survival rate. Under hypoxic conditions, the expression of VEGF, p65 and TGF-β2 increased. Addition of ATRA significantly attenuated the expression of VEGF, p65 and TGF-β2. There was a corresponding variation of NF-κB/DNA binding activities. In addition, ATRA stimulated Scpep1 expression under normoxic and hypoxia condition. Furthermore, TGF-β2 interacted with Scpep1. Conclusions: This study indicated that ATRA may attenuate hypoxia-induced injury in NRK52E cells via inhibiting NF-κB/VEGF and TGF-β2/VEGF pathway.
Mesenchymal Stem Cells Alleviate LPS-Induced Acute Lung Injury in Mice by MiR-142a-5p-Controlled Pulmonary Endothelial Cell Autophagy
Authors: Zhou Zichao , You Zhijian
Published: January 2016

Background/Aims: Damages of pulmonary endothelial cells (PECs) represent a critical pathological process during acute lung injury (ALI), and precede pulmonary epithelial cell injury, and long-term lung dysfunction. Transplantation of mesenchymal stem cells (MSCs) has proven therapeutic effects on ALI, whereas the underlying mechanisms remain ill-defined. Method: We transplanted MSCs in mice and then induced ALI using Lipopolysaccharides (LPS). We analyzed the changes in permeability index and lung histology. Mouse PECs were isolated by flow cytometry based on CD31 expression and then analyzed for autophagy-associated factors LC3 and Beclin-1 by Western blot. Beclin-1 mRNA was determined by RT-qPCR. In vitro, we performed bioinformatics analyses to identify the MSCs-regulated miRNAs that target Beclin-1, and confirmed that the binding was functional by 3'-UTR luciferase reporter assay. Results: We found that MSCs transplantation significantly reduced the severity of LPS-induced ALI in mice. MSCs increased autophagy of PECs to promote PEC survival. MSCs increased Beclin-1 protein but not mRNA. MiR-142a-5p was found to target the 3'-UTR of Beclin-1 mRNA to inhibit its protein translation in PECs. MSCs reduced the levels of miR-142a-5p in PECs from LPS-treated mice. Conclusion: MSCs may alleviate LPS-ALI through downregulation of miR-142a-5p, which allows PECs to increase Beclin-1-mediated cell autophagy.
Axial Length and Associated Factors in Children: The Shandong Children Eye Study
Authors: Lu Tai Liang , Wu Jian Feng , Ye Xiang , Hu Yuan Yuan , Wu Hui , Sun Wei , Guo Da Dong , Wang Xing Rong , Bi Hong Sheng , Jonas Jost B.
Published: January 2016

Purpose: To investigate ocular axial length (AL) in Chinese children. Methods: The Shandong Children Eye Study included 6,364 children aged 4-18 years. Results: Longer AL (mean 23.45 ± 1.20 mm, range 18.80-28.59 mm) was associated (multivariate analysis; correlation coefficient r2: 0.61) with older age (p < 0.001, standardized correlation coefficient β = 0.35, unstandardized regression coefficient B = 0.13, 95% confidence interval [CI] 0.12, 0.15), male gender (p < 0.001, β = -0.24, B = -0.10, 95% CI -0.29, -0.19), urban region (p < 0.001, β = 0.10, B = 0.25, 95% CI 0.20, 0.31), body height (p < 0.001, β = 0.22, B = 0.02, 95% CI 0.01, 0.02), maternal education (p < 0.001, β = 0.07, B = 0.07, 95% CI 0.05, 0.10), paternal myopia (p < 0.001, β = 0.09, B = 0.26, 95% CI 0.20, 0.33), maternal myopia (p < 0.001, β = 0.08, B = 0.23, 95% CI 0.17, 0.30), more time spent indoors reading/writing (p < 0.001, β = 0.05, B = 0.03, 95% CI 0.02, 0.04), less time spent outdoors (p = 0.005, β = -0.03, B = -0.01, 95% CI -0.02, -0.003), longer corneal curvature radius (p < 0.001, β = 0.36, B = 1.63, 95% CI 1.53, 1.74) and higher intraocular pressure (p = 0.008, β = 0.03, B = 0.01, 95% CI 0.004, 0.02). High axial myopia (AL ≥26.0 mm) present in 202 children (3.4 ± 0.2%, 95% CI 2.92, 3.84) was associated with less time spent outdoors (p = 0.002, odds ratio 0.92, 95% CI 0.87, 0.97) in multivariate analysis. Conclusions: In children in the less developed Eastern Chinese province of Shandong, the prevalence of high axial myopia was >10% among 16-year-olds. A modifiable factor associated with higher prevalence of high axial myopia was less time spent outdoors.
The Importance of Supportive Care with Tranexamic Acid in Crimean-Congo Hemorrhagic Fever
Authors: Kaya Selçuk , Aksoy Firdevs , Kenç Nurten Nur , Eroğlu Ahmet , Köksal İftihar
Published: January 2016

This report describes a case of Crimean-Congo hemorrhagic fever with widespread hemorrhages and multiple organ dysfunction syndrome in a 46-year-old patient from an endemic region. Although the patient had numerous poor prognostic factors, he was discharged in a healthy condition after 17 days of hospitalization with close monitoring and supportive care. Tranexamic acid was successfully used together with other supportive treatments.
Human Papillomavirus 16 Physical Status and the TP53 Codon 72 Polymorphism in Japanese HPV-Positive Oropharyngeal Cancer Patients
Authors: Saito Yuki , Ando Mizuo , Fujimoto Chisato , Omura Go , Kobayashi Kenya , Asakage Takahiro , Yamasoba Tatsuya
Published: January 2016

Background: In human papillomavirus (HPV)-induced carcinogenesis, the arginine (Arg) allele of the TP53 codon 72 polymorphism binds more efficiently to the HPV E6 oncoprotein than the proline (Pro) allele. We investigated the physical status of HPV-16 DNA and the TP53 codon 72 polymorphism in oropharyngeal squamous cell carcinoma (OPSCC). Methods: Tumor samples from 70 p16-positive OPSCC patients were tested for HPV-16 physical status by examining the E2 and E6 open reading frames. The TP53 codon 72 polymorphism was screened by direct sequencing. Results: Of 70 patients, 53 were E6 positive, 29 had integrated forms of HPV-16 DNA, and 24 had mixed or episomal forms. Furthermore, 44 carried the Arg/Arg or Arg/Pro genotype, 3 carried the Pro/Pro genotype, and in 6 patients we were unable to obtain sequencing data. Conclusions: HPV-16 physical status was heterogeneous in our OPSCC patients. Most OPSCC patients had the TP53 Arg/Arg or Arg/Pro genotype.
Thyroid Disease and Oral Lichen Planus as Comorbidity: A Prospective Case-Control Study
Authors: Garcia-Pola Maria José , Llorente-Pendás Santiago , Seoane-Romero Juan M. , Berasaluce Maria Jesus , García-Martín José Manuel
Published: January 2016

Background: Thyroid disease has been mentioned to have a possible relation to the development of oral lichen planus (OLP). Objective: Because goiter is considered endemic in many countries, we proposed to determine whether thyroid disease constitutes a comorbidity of OLP. Methods: Two hundred and fifteen patients diagnosed as having OLP were evaluated concerning their serum thyroid-stimulating hormone and thyroxine (T4) levels. The results were contrasted with those obtained in control series of the same number of subjects matched for age and sex. Results: Diagnosis of thyroid disease was present in 15.3% of OLP patients (33/215) and in 5.2% (12/215) of the control group. In relation to OLP patients, the odds ratio of presence of thyroid disorders was 3.06 and that of using levothyroxine medication 3.21. Conclusions: In the present study, OLP patients were associated with thyroid disease, specifically with hypothyroidism. Because most thyroid patients need T4 treatment, our findings confirmed that OLP and thyroid disease could be comorbidities.
Pediatric Awake Craniotomy for Brain Lesions
Authors: Akay Ali , Rükşen Mete , Çetin H. Yurday , Seval H. Özer , İşlekel Sertaç
Published: January 2016

Awake craniotomy is a special method to prevent motor deficits during the resection of lesions that are located in, or close to, functional areas. Although it is more commonly performed in adult patients, reports of pediatric cases undergoing awake craniotomy are limited in the literature. In our clinic, where we frequently use awake craniotomy in adult patients, we performed this method in 2 selected pediatric cases for lesion surgery. At an early age, these 2 cases diagnosed with epilepsy presented cerebral lesions, but since the lesions enclosed functional areas, surgical resection was not regarded as a treatment option at this time. In these 2 pediatric cases, we successfully completed lesion surgery with awake craniotomy. The method and the techniques employed during surgery are presented concomitant with other reports in the literature.
Measuring Contact Pressure of Lower Extremities in Patients Undergoing Robot-Assisted Radical Prostatectomy
Authors: Yamada Yuta , Fujimura Tetsuya , Fukuhara Hiroshi , Miyagawa Jimpei , Miyazaki Hideyo , Nakagawa Tohru , Kume Haruki , Homma Yukio
Published: January 2016

Introduction: Lower extremity neuropathy (LEN) is a well-recognized complication that arises during robotic surgeries in lithotomy-Trendelenburg position (L-T position). It is caused by persistent compression to the nerves. Our aim was to investigate the levels of compression to the nerves by measuring the contact pressure of the lower extremities in men undergoing robot-assisted radical prostatectomy (RARP). Materials and Methods: A total of 138 patients undergoing RARP were enrolled prospectively. The contact pressure of the lower extremities was measured using a portable contact pressure measurement device (Cello®, Cape Co., Kanagawa, Japan) in lithotomy position (L position) and L-T position preoperatively, and in L position postoperatively. Patient positioning was readjusted when the preoperative contact pressure was over 32 mm Hg. The contact pressure was compared between pre- and postoperative values. Results: A total of 33 patients (23.9%) needed readjustment of positioning due to high contact pressure. No one developed LEN. The contact pressure in the L position significantly increased postoperatively (right: 21.4 mm Hg, p < 0.0001; left: 18.6 mm Hg, p = 0.0011). A high body mass index (BMI) was associated with a postoperative increment of pressure in the L position. Conclusions: The contact pressure of the lower extremities increased after RARP and the increase was correlated with BMI.
Higher Levels of Cystatin C Are Associated with Extracranial Carotid Artery Steno-Occlusive Disease in Patients with Noncardioembolic Ischemic Stroke
Authors: Umemura Toshitaka , Kawamura Takahiko , Mashita Shinichi , Kameyama Takashi , Sobue Gen
Published: January 2016

Background: Large artery atherosclerosis is a major cause of ischemic stroke worldwide. Differential biomarker profiles associated with extra- and intracranial atherosclerosis are a topic of considerable interest. Cystatin C (CysC), a marker of renal function, is a risk factor for cardiovascular disease. Aim: We sought to determine whether CysC levels were associated with extra- and intracranial large artery stenosis (LAS) in patients with acute ischemic stroke. Methods: We retrospectively analyzed data of acute noncardioembolic ischemic stroke patients who were admitted to our stroke center within 5 days from symptom onset. Serum CysC levels were measured using latex agglutination turbidimetric immunoassay. Extra- and intracranial LAS were defined as ≥50% diameter stenosis or occlusion of the relevant internal carotid artery (ICA) and/or middle cerebral artery (MCA) using carotid echography and volume rendering on magnetic resonance angiography. Multivariate logistic analyses were used to assess the association between CysC levels and LAS after adjustment for potential confounders. Results: Of 205 patients (mean age 70.2 years), 76 (37.1%) had LAS. The distribution of LAS was 29 extracranial ICA, 34 intracranial ICA/MCA (8 ICA only, 25 MCA only, 1 ICA+MCA) and 13 tandem stenosis (both extracranial ICA and intracranial ICA/MCA). Levels of CysC were higher in patients with extracranial ICA stenosis than in those with intracranial ICA/MCA stenosis (1.23 ± 0.33 vs. 0.97 ± 0.21 mg/l, p < 0.001). In multivariate analysis, the highest CysC tertile (>1.04 mg/l) was significantly associated with extracranial ICA stenosis (adjusted odds ratio [OR] 5.01, 95% confidence interval [CI] 1.51-16.63, p = 0.009) after adjustment for age, sex, diabetes, chronic kidney disease, current smoking, systolic blood pressure, HDL cholesterol, high-sensitivity C-reactive protein (hs-CRP) and premorbid lipid-lowering drugs use. When CysC was considered as a continuous variable, 1 SD increase in CysC was significantly associated with extracranial ICA stenosis (adjusted OR 3.01, 95% CI 1.58-5.72, p = 0.001). However, there were no significant associations between CysC levels and intracranial ICA/MCA stenosis. In addition, CysC levels showed a weak but statistically significant correlation with hs-CRP levels (r = 0.195, p = 0.021). Using receiver operating characteristic curve analysis, CysC value displayed good performance in discriminating extracranial ICA stenosis (c-statistic 0.79, 95% CI 0.69-0.89, p < 0.001). Conclusions: This preliminary study suggests that higher levels of CysC were independently associated with symptomatic extracranial ICA stenosis, but not with intracranial ICA/MCA stenosis in patients with noncardioembolic stroke. Our findings provide new insights into the link between serum CysC and carotid atherosclerosis.
Serum Levels of 25-Hydroxyvitamin D and Time to Natural Pregnancy
Authors: Somigliana Edgardo , Paffoni Alessio , Lattuada Debora , Colciaghi Barbara , Filippi Francesca , La Vecchia Irene , Tirelli Amedea , Baffero Giulia Maria , Persico Nicola , Viganò Paola , Bolis Giorgio , Fedele Luigi
Published: January 2016

Aim: The aim of this study was to evaluate whether natural fertility is related to serum 25-hydroxyvitamin D (25-OH-vitamin D) levels. Methods: A nested case-control study was designed from a prospective cohort of pregnant women undergoing first trimester screening for aneuploidies. Cases included women seeking pregnancy for 12-24 months. Controls were the subsequent age-matched women conceiving in less than 1 year. We excluded women aged ≥40 or <18 years, those assuming supplementary products that included vitamin D before or during pregnancy, those with irregular menstrual cycles or known causes of subfertility, those conceiving through assisted reproductive techniques or requiring ovarian stimulation and those who were overweight or obese. A quantitative detection of serum 25-OH-vitamin D and patients' interview were performed. Results: Seventy-three cases and 73 matched controls were selected. The mean ± SD serum 25-OH-vitamin D was 21.2 ± 6.8 and 19.7 ± 7.3 ng/ml, respectively (p = 0.16). The number (%) of women with serum levels <20 ng/ml (vitamin D insufficiency) was 34 (47%) and 37 (51%), respectively (p = 0.73). The adjusted OR of longer time to pregnancy in women with vitamin D insufficiency was 0.84 (95% CI 0.42-1.66). Conclusions: Our study does not support a crucial role of 25-OH-vitamin D in natural fertility.
A Case of Infantile Kyrle-Flegel Disease in a 6-Year-Old Yemeni Girl
Authors: Alshami Mohammad Ali , Mohana Mona Jameel
Published: January 2016

Kyrle disease (KD) and Flegel disease (FD) are rare variants of primary perforating dermatoses, characterized by transepidermal elimination of abnormal endogenous materials. We describe a 6-year-old Yemeni girl with a 2-year history of generalized asymptomatic, small, reddish-brown keratotic papules with a lenticular central keratotic plug. Although these features are synonymous with FD, the histology of a punch biopsy was consistent with KD. The patient was otherwise healthy, and no family members had a history of similar diseases. The patient was diagnosed with KD-FD, owing to the manifestation of features associated with both diseases.
Arte contemporânea e arquivo: reflexões sobre a 3a Bienal da Bahia
Authors: Pato Ana Mattos Porto
Published: January 2016

O presente artigo pretende discutir a invisibilidade dos arquivos no Brasil e o papel de artistas e curadores na investigação de novos modelos de uso para os espaços de memória, a partir do contato com a arte e de projetos de cooperação entre instituições culturais. Para tratar da questão, o foco, aqui, será analisar o projeto de realização da 3a Bienal da Bahia e, em particular, uma das suas estruturas temáticas, dedicada à psicologia do testemunho e ao desenvolvimento de ações e pesquisas em torno de arquivos. Com enfoque para a experiência com artistas no Arquivo Público do Estado da Bahia, e os projetos desenvolvidos pelos artistas Eustáquio Neves, Ícaro Lira, Maria Magdalena Campos-Pons e Paulo Nazareth a partir do acervo do Museu Antropológico e Etnográfico Estácio de Lima.
Produção de conhecimento e tradições de pesquisa na Faculdade de Filosofia, Ciências e Letras – USP (1934-1968)
Authors: Silva Márcia Regina Barros da , Dantes Maria Amélia Mascarenhas
Published: January 2016

O objetivo deste artigo é apresentar os primeiros resultados do projeto Memória científica e acadêmica: preservação documental da Faculdade de Filosofia, Ciências e Letras da USP (1934-1968), aprovado no Edital Memória USP 2012, da Pró-reitoria de Cultura e Extensão. Tal projeto pretende caracterizar tradições de pesquisa presentes nas primeiras décadas da Faculdade de Filosofia, Ciências e Letras da USP, criada em 1934, até a reforma universitária de 1969. A proposta está centrada na análise das teses e dissertações que foram orientadas e defendidas nas várias áreas científicas que compunham a faculdade, por meio da busca do que chamamos de ‘genealogias científicas’, uma forma de acompanhar os docentes orientadores e seus alunos. Desta maneira busca-se verificar os alunos que permaneceram na universidade e, que por sua vez, também se tornaram orientadores em suas respectivas áreas.
I Seminário de conservação de igrejas e arte sacra de Santa Catarina
Authors: Kremer Karen , Gondim Letícia da Silva , Melo Marcelino Donizeth de
Published: January 2016

O I Seminário de Conservação de Igrejas e Arte Sacra de Santa Catarina foi pensado de forma a valorizar os bens culturais sacros móveis, imóveis e integrados da Igreja Católica no estado. O objetivo foi chamar a atenção de párocos, padres e administradores das paróquias e igrejas quanto à importância da conservação preventiva desses bens.
Revista CPC - 10 anos
Authors: Camargo Mônica Junqueira de
Published: January 2016

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A Residência Franco de Mello em três tempos: da domesticidade belle époque ao Centro de Cultura, Memória e Estudos da Diversidade Sexual do Estado de São Paulo
Authors: Silva Joana Mello de Carvalho e , Ferreira Pedro Beresin Schleder , Raghi Camila , Ferroni Eduardo , Hereñu Pablo
Published: January 2016

As novas instalações do Centro de Cultura, Memória e Estudos da Diversidade Sexual do Estado de São Paulo serão implantadas junto a um dos últimos remanescentes da arquitetura residencial que marcou a paisagem da Avenida Paulista, hoje um eixo urbano consolidado, espaço público por excelência de profundo significado político e cultural. Seu projeto dialoga com os desafios da cidade e sociedade contemporâneas e com a história da cidade, da arquitetura e da família. Por isso, o presente artigo recupera a história da Residência Franco de Mello, indicando os seus usos e vivências; a autenticidade de seus elementos; as alterações, reformas e ampliações, bem como a sua ambientação urbana, informações estas que subsidiaram as decisões dos projetos de arquitetura, paisagismo e restauro também aqui apresentados.
A construção da história e a legitimação da memória no processo de preservação do Casarão Pau Preto
Authors: Jesus Carlos Gustavo Nóbrega de
Published: January 2016

O seguinte artigo tem o intuito de discutir a questão do patrimônio cultural e suas relações com as políticas públicas de preservação e legitimação da memória. Para tanto, partiu-se do estudo do processo de tombamento de um bem edificado localizado na cidade de Indaiatuba, interior do estado de São Paulo, construído entre 1810 e 1812, denominado Casarão Pau Preto. A partir de escritos de memorialistas locais e laudos técnicos de especialistas, buscou-se levantar algumas evidências da relação da conservação do imóvel com as políticas públicas de preservação patrimonial e com o empenho de construção de uma memória e legitimação da história da cidade, em torno da consagração de tal patrimônio histórico.
ROLAND BARTHES E AS REPRESENTAÇÕES DA ALTERIDADE
Authors: Silva Maria Luiza Berwanger da
Published: January 2016

Com base na leitura do texto L’Étrangère, de Roland Barthes, este artigo examina representações do estrangeiro emergentes da produção teórico-crítica desse intelectual francês. Considera sobretudo a figura do Outro mediada pela amizade intelectual e pessoal que estabelece com Julia Kristeva. Para tanto, o artigo evidencia a contribuição de Julia Kristeva para o avanço da Semiologia Francesa, representando-a pelo projeto de renovação de paradigmas semiológicos cristalizados que impedem, segundo Barthes, a valorização da diferença. Este artigo também estuda textos de homenagem de Julia Kristeva a Roland Barthes, neles sublinhando a crítica da reciprocidade como critério de valor e « noyau dur » da reflexão barthesiana. Assim, o presente artigo estabelece um jogo de cartografias intelectuais e afetivas entre os dois semiólogos que enfatizam a recepção favorável à Alteridade articulada pelo pensamento plural (nacional e transnacional) de Roland Barthes.
DERRIDA E BARTHES: POR ENTRE SINGULARIDADES E PLURALIDADES
Authors: Campos Raquel
Published: January 2016

Através da homenagem feita por Derrida a Barthes em seu ensaio “As mortes de Roland Barthes”, o artigo pretende traçar os pontos de encontro entre os dois pensadores em meio à pluralidade de suas ideias e na singularidade das mesmas. “Essa singularidade penetrada me alcança de um golpe, me fere ou me assassina e, em princípio, parece olhar diretamente para mim. […] A mim se dirige a singularidade absoluta do outro” (2008, p.275), como afirma Derrida em seu ensaio. Os pontos intransponíveis entre um indivíduo e outro criam as singularidades, ao mesmo tempo em que contribuem, aporeticamente, à pluralidade de caminhos, possibilidades e ideias. O aparente paradoxo entre esses dois pontos e a coexistência de ambos em um mesmo espaço serão analisados, na tentativa de encontrar consonâncias entre dois tão importantes pensadores, distintos, mas que, por vezes, trabalharam em suas respectivas obras com questões similares.
DESPLAZAMIENTOS Y TRANSFORMACIONES DE LA CRÍTICA EN ROLAND BARTHES
Authors: Hidalgo Max
Published: January 2016

El artículo explora un caso (práctica e imagen) de la crítica moderna a través del análisis de la escritura barthesiana y de la posición de campo ocupada por el autor. En Barthes, la legitimidad de la crítica, reivindicada desde su ausencia de autoridad, va ligada a un ejercicio de desplazamiento y de transformación de los discursos existentes. El estudio de su recorrido permite ver cómo la transformación del campo intelectual de los años setenta y su entrada en el Collège de France supondrán un antes y un después en las estrategias de intervención del crítico.
DE PROUST A BARTHES: A BUSCA PELA LITERATURA
Authors: Cavalcanti e Silva Carla
Published: January 2016

Em seu ensaio "Durante muito tempo, fui dormir cedo", publicado em o Rumor da língua, Barthes ressalta uma das características mais importantes da obra proustiana: não se trata nem de Romance, nem de Ensaio, mas de uma terceira forma. Essa forma seria a única possível a dar estrutura ao romance proustiano, que destaca, dentre outros elementos, a busca do próprio narrador por tornar-se escritor. Ao discorrer sobre essa nova lógica proustiana, Barthes se identifica com Marcel Proust, pois naquele momento, o crítico busca uma nova forma de escrever (a literatura) tendo como ponto de partida seu estado de luto, ou para usarmos um termo barthesiano, de acedia. O intuito desta comunicação é percorrer os escritos barthesianos que discorrem notadamente sobre Marcel Proust para analisar a importância do escritor francês na produção crítica e na escrita de Roland Barthes. Para tanto, propomos comentar fundamentalmente as obras: Novos ensaios críticos, O rumor da língua e APreparação do romance.
“UMA PAIXÃO DO NEUTRO”: RELAÇÕES ENTRE NEUTRO E SUBJETIVIDADE
Authors: Silva Arthur Dantas
Published: January 2016

Em sua aula inaugural da cadeira de Semiologia Literária do Collège de France, Roland Barthes afirma que um ensino como o praticado pela instituição deve admitir sempre uma fantasia. Em seu segundo curso ministrado, O neutro (1977-1978), Barthes reafirma o compromisso de colocar a aula sempre à disposição de um desejo: “Lembrar aula inaugural: promessa de que a cada ano o curso, a pesquisa, partiria claramente de uma fantasia pessoal. Em resumo: desejo o Neutro, logo postulo o Neutro. Quem deseja postula (alucina)” (BARTHES, 2003, p. 30). Ora, como desejo (desejo de neutro), o neutro pressupõe um sujeito que, a priori, talvez, pudesse negá-lo, impossibilitando-o constituir-se como neutro, tendo em vista que, da maneira como Barthes o postula, o neutro seria a suspensão de qualquer paradigma (qualquer imposição arrogante de sentido, mesmo atrelada à uma edificação subjetiva). A definição do neutro como desejo não exclui, entretanto, a presença do sujeito. Sendo assim, o trabalho pretende investigar como o neutro e esse sujeito que o deseja estão relacionados, quais as implicações que isso acarreta para a postulação do “objeto” feito por Barthes durante o curso e como esse neutro está presente (ou se deseja que esteja presente) na vida do próprio sujeito.