bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of sleep quality on the severity of perimenstrual symptoms in Japanese female students: a cross-sectional, online survey
Authors: Oda Sakura , Maeda Noriaki , Tashiro Tsubasa , Mizuta Rami , Komiya Makoto , Arima Satoshi , Nagasawa Takaaki , Naito Koichi , Urabe Yukio
Published: February 2025

Objectives

To investigate the relationship between sleep quality and perimenstrual symptoms among Japanese female students.

Design

Observational, cross-sectional, online survey

Setting

We used an online questionnaire to collate responses from Japanese female students on sleep quality and perimenstrual symptoms.

Participants

A total of 298 female students aged 18–25 years in Japan were included in this study.

Primary and secondary outcome measures

The Menstrual Distress Questionnaire (MDQ) was used to assess the severity of perimenstrual symptoms for three periods—premenstrual, menstrual and postmenstrual—and the Japanese version of the Pittsburgh Sleep Quality Index was used to assess sleep quality. The MDQ scores were compared between two groups (normal-sleep quality and low-sleep quality) using Mann-Whitney U test. In addition, multiple logistic regression analysis was performed, and the MDQ subscales that showed significant differences between the groups were used as independent variables. The MDQ subscale that was strongly associated with sleep quality was calculated.

Results

Of the female students, 160 were classified into the normal-sleep quality group and 138 into the low-sleep quality group. The total MDQ scores were significantly higher in the low-sleep quality group at all phases of the menstrual cycle (respectively p<0.05). Among the MDQ subscales, ‘pain’ during menstruation and ‘concentration’ in the premenstrual and postmenstrual stages were associated with sleep quality (respectively p<0.05).

Conclusions

Improving sleep quality was one possible strategy to reduce the severity of perimenstrual symptoms. These results may provide useful information for Japanese female students who suffer from perimenstrual symptoms.
Korean patients with hereditary cancer: a prospective multicentre cohort study protocol exploring psychosocial and health outcomes
Authors: Kim Jun-Kyu , Jang Mi-Ae , Park Jong Eun , Won Dongju , Ha Jung-Sook , Kim Kyoung-Bo , Park Boyoung , Kong Sun-Young
Published: February 2025

Introduction

Although genetic testing for hereditary cancers is increasing, data on health attitudes based on genetic pathogenicity are limited. This cohort study aims to establish three subcohorts based on genetic testing results to assess the health impact of genetic variations. This study evaluates changes in participant quality of life (QoL), unmet needs and mental health over time based on their genetic variant status.

Methods and analysis

This prospective cohort study will recruit 1435 patients with suspected hereditary cancer who have undergoneBRCA1/2or next-generation sequencing (NGS) testing. The study began in July 2023 and will continue until December 2027. By 2026, participants will be surveyed up to four times annually during their outpatient visits. The survey consists of 342 items across 5 domains: comorbidities (96), health behaviours (80), QoL (41), unmet needs (75) and mental health (50). Data were collected using 11 validated surveys. In addition, information on the chronic diseases, cancer diagnoses, medical history and treatment history of participants will be extracted from their electronic medical records to analyse their health status and cancer treatment experiences. Genetic variant data fromBRCA1/2and NGS will be used to classify participants into three subcohorts: pathogenic variants, variants of uncertain significance and undetectable mutations. A three-generation pedigree that includes details such as the year of cancer diagnosis, age at diagnosis, cancer type, survival status of family members and age at death will be constructed for each participant. The collected data will be linked to secondary sources such as cancer registries and National Health Insurance Service data to provide a comprehensive analysis of the impact of hereditary cancer on health and survival.

Ethics and dissemination

The study protocol was approved by all the Ethics Committees: the National Cancer Center IRB (NCC2023-0179), the Samsung Medical Center IRB (SMC2023-09-057), the Yonsei University Health System, Severance Hospital IRB (4-2023-0627), the Hanyang University Guri Hospital IRB (GURI2023-08-021) and the Keimyung University IRB (DSMC IRB 2024-05-048). The study outcomes will be disseminated through conference presentations, peer-reviewed publications and social media.

Trial registration number

KCT0009460.
Oncolytic virus OH2 extends survival in patients with PD-1 pretreated melanoma: phase Ia/Ib trial results and biomarker insights
Authors: Wang Xuan , Tian Hui , Chi Zhihong , Si Lu , Sheng Xinan , Hu Han , Gu Xiangyong , Li Siming , Li Caili , Lian Bin , Zhou Li , Mao Lili , Tang Bixia , Yan Xieqiao , Wei Xiaoting , Li Juan , Liu Binlei , Guo Jun , Kong Yan , Cui Chuanliang
Published: February 2025

Background

OH2 is an oncolytic virus derived from herpes simplex virus type 2. A phase Ia/Ib clinical trial in China was conducted in patients with unresected stage III–IV melanoma, the majority of whom had the acral type, to assess the safety and preliminary efficacy of OH2.

Methods

The trial enrolled patients with histologically confirmed unresectable stage III or advanced stage IV melanoma. In phase Ia, nine patients received OH2 single-dose treatment across three dose levels (106, 107, and 108CCID50/mL, where CCID50represents cell culture infectious dose 50%) while six patients underwent multidose therapy. Phase Ib expanded the proposed dose. Antitumor efficacy was evaluated using the Response Evaluation Criteria in Solid Tumors and immune-RECIST guidelines.NCT04386967is the clinical trial identifier.

Results

All 44 patients were enrolled. OH2 was well tolerated without serious adverse events (AEs) or deaths reported. No Grade 3 or higher treatment-related AEs occurred. In phase Ia, the 1-year survival rate was 92.9% (95% CI, 59.1% to 99.0%), with a median overall survival of 28.9 months (95% CI, 12.7 to not reached). In phase Ib, 10 patients achieved immune-partial response (iPR)/partial response (PR), yielding an objective response rate (ORR) of 37.0% (95% CI, 19.4% to 57.6%), with 6 patients still responding. The rate of the durable response (PR or complete response lasting at least 6 months) was at least 29.6% (8/27). Notably, 7 of 12 III–IVM1a patients who previously received programmed cell death protein-1 (PD-1) therapy achieved iPR/PR, with an ORR of 58.3% (95% CI, 27.7% to 84.8%) and a disease control rate of 75.0% (95% CI, 42.8% to 94.5%). Biomarker analysis indicated that elevated baseline neutrophil activation state correlated with poorer clinical outcomes. A phase III clinical trial is ongoing in China (NCT05868707).

Conclusions

OH2 oncolytic virotherapy exhibited a favorable safety profile without dose-limiting toxicities (DLTs) and demonstrated durable antitumor efficacy in patients with melanoma, especially in those who had progressed on anti-PD-1 treatment.

Trial registration number

ClinicalTrials.gov identifierNCT04386967.
Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality
Authors: Rahnama Ruyan , Kizerwetter Monika , Yang Huilin , Christodoulou Ilias , Guaraca Christian , Holl Natalie Jordan , Choe Jun , Vorri Stamatia C , Zinsky Megan , Jones Danielle G , Garcia Espinoza Nikol , Kuo Yun-Huai , Zahurak Marianna , Varadhan Ravi , Spangler Jamie B. , Bonifant Challice L.
Published: February 2025

Background

Natural Killer (NK) cells have intrinsic anticancer activity that can be redirected toward acute myeloid leukemia (AML) with chimeric antigen receptor (CAR) engineering. Here, we study the functional consequences of CAR binding affinity and targeted epitope on CAR-NK cell activation, cytolytic synapse formation, and antitumor activity.

Methods

We characterized NK-92 and primary NK cell populations expressing variant affinity AML-specific CARs containing single-chain variable fragments (scFvs, 26292 or 7G3) targeting two epitopes on CD123. 26292 affinity variants were discovered through directed evolution of an error-prone mutagenic library, while 7G3 affinity variants were previously reported. The resulting CAR-NK cell panel was studied with in vitro binding, activation, and cytotoxicity studies and in mouse xenograft models.

Results

26292 and 7G3 CARs of variable CD123 binding affinities were highly expressed in NK cells and conferred antigen-specific activation in vitro. High-resolution imaging demonstrated greater clustering of high-affinity 7G3 CAR-NK cells and consequent AML target cell death in a short-term time lapse. Low-affinity 7G3 CAR-NK cells exhibited enhanced antigen density discrimination with greater membrane-proximal signaling, cytokine production, and cytotoxicity. In longer-term assays, low-affinity 7G3 CAR-NK cells demonstrated more sustained killing of AML cells. In vivo testing highlighted greater expansion of low-affinity 7G3 CAR-NK cells in two xenograft models.

Conclusions

Expression of 26292 and 7G3 CARs with a range of CD123 binding affinities in NK cells leads to antigen-specific activation and cytotoxicity against AML. Affinity-based differences in functional activation and antitumor activity are dependent on time course and are scFv/epitope specific.
UBE2T/CDC42/CD276 signaling axis mediates brain metastasis of triple-negative breast cancer via lysosomal autophagy
Authors: Shi Liang Liang , Chen Yan , Xie Ming Xing , Chen Qian Zhi , Qiao Xin Wei , Cheng Qi Hong , Li Lin , Fu Rong , Liang Tao , Jiang Xiaobing , Wang Min Jie , Yao Jin , Li Jun Jun
Published: February 2025

Background

Advanced triple-negative breast cancer (TNBC) is prone to brain metastasis (BrM). The precise molecular mechanism responsible for this phenomenon has not yet been completely established, so it is vital to comprehend the molecular mechanism behind it.

Methods

The protein chip analysis was conducted to identify any abnormal UBE2T protein expression in TNBC, especially BrM. Here, we used public databases and bioinformatics analysis as well as clinical samples from different cohorts to investigate the interrelationship between UBE2T/CDC42/CD276. This predicted relationship was then repeatedly validated using different in vivo and in vitro experimental methods. Additionally, multiple experimental approaches were implemented, encompassing western blotting, Co-IP, GST pull-down, flow cytometry, mass spectrometry, immunofluorescence, immunohistochemistry, and qRT-PCR to reveal the molecular mechanism of UBE2T-mediated immune escape and BrM.

Results

Our results indicate that expressed at elevated levels in breast cancer, UBE2T is negatively linked to patient prognosis, especially in BrM of TNBC. Data from clinical samples from our different cohorts and TCGA indicate a significant correlation between UBE2T and immunosuppression. Mechanistically, UBE2T directly interacts with CDC42, promoting its K48-linked polyubiquitination and proteasomal degradation, thereby inhibiting CDC42 from degrading CD276 via the autophagy-lysosomal pathway, indirectly upregulating CD276 and thereby impairing the CD8+T cells function, ultimately mediating tumor immune escape and BrM. Finally, animal experimental results also showed that inhibition of UBE2T elevated the TNBC sensitivity to immune checkpoint CD276 blockade and inhibited BrM of TNBC.

Conclusions

In conclusion, our results indicate a new mechanism whereby UBE2T-mediated ubiquitination positively controls the UBE2T/CDC42/CD276 axis to upregulate tumor cell expression of CD276 and thereby impair CD8+T cells function, ultimately leading to tumor cell immune escape and BrM.
Doxycycline prophylaxis for bacterial sexually transmitted infection prevention in Vietnam: awareness, attitudes and willingness to use among men who have sex with men using HIV-PrEP
Authors: Bui Hao T M , Adamson Paul C , Klausner Jeffrey D , Le Giang M. , Gorbach Pamina M
Published: February 2025

Objectives

Doxycycline prophylaxis shows promise for bacterial sexually transmitted infection (STI) prevention, but data primarily come from high-income countries. This study assessed awareness, willingness and factors associated with willingness to use doxycycline for bacterial STI prevention among men who have sex with men (MSM) using HIV pre-exposure prophylaxis (HIV-PrEP) in Vietnam.

Methods

Between 25 January and 4 February 2024, a cross-sectional study recruited males aged ≥18 years who reported having sex with men in the past 12 months from 11 HIV-PrEP clinics in Hanoi and Ho Chi Minh City. Self-administered surveys were conducted, and multivariable logistic regression was applied to identify factors associated with willingness to use doxycycline prophylaxis.

Results

Among 350 participants, the median age was 25 (IQR 21–30), and 10.6% self-reported a bacterial STI diagnosis in the past 12 months. In the previous 6 months, the median number of sex partners was 2 (IQR 1–4), 53.1% reported condomless anal sex. Awareness of doxycycline was low (20.2%; 65/322); however, 75.4% (264/350) expressed willingness to use it, with 63.6% (168/264) preferring doxyPrEP. Participants who disclosed HIV-PrEP use to all sex partners (adjusted OR (aOR) 4.17; 95% CI 1.84, 9.46) and those with higher perceived STI risk (aOR 1.12; 95% CI 1.03, 1.22) were more likely to report willingness to use doxycycline prophylaxis. Concerns about daily medication (aOR 0.43; 95% CI 0.24, 0.81) and fear of judgement from peers (aOR 0.41; 95% CI 0.21, 0.81) were associated with lower willingness.

Conclusions

Knowledge of doxycycline prophylaxis among MSM on HIV-PrEP in Vietnam was low. However, most expressed willingness to use it, with two-thirds preferring doxyPrEP. Findings highlight the need to disseminate information on doxycycline prophylaxis for bacterial STI prevention, monitoring the usage and evaluating comparative effectiveness of doxyPEP and doxyPrEP to guide implementation efforts in Vietnam.
FRACTIONAL INTEGRAL INEQUALITIES OF OSTROWSKI AND HADAMARD TYPE VIA k-ANALOGUES OF CAPUTO DERIVATIVES
Authors: LIU YONGHONG , FARID GHULAM , ALKHALIFA LOAY , NAZEER WAQAS
Published: February 2025

Fractional derivative operators are important tools for generalizing concepts and theories based on usual derivatives and integrals. Many integral and derivative inequalities in the literature can be analyzed for fractional integrals and derivatives. In this paper, well-known Ostrowski and Hadamard integral inequalities are established by using fractional derivatives. These inequalities estimate the bounds of k-analogues of Caputo fractional derivatives (CFk-D). An Ostrowski–Grüss-type inequality is also established under new constraints. Applications of these inequalities are given at fixed points, and various already published results are obtained.
DYNAMICAL BEHAVIORS OF A FRACTIONAL-ORDER PREDATOR–PREY MODEL: INSIGHTS INTO MULTIPLE PREDATORS COMPETING FOR A SINGLE PREY
Authors: PANIGORO HASAN S. , RAHMI EMLI , BONYAH EBENEZER , AKGÜL ALI , JOSE SAYOOJ ABY
Published: February 2025

In this paper, we investigate the dynamical behaviors of a modified Bazykin-type two predator-one prey model involving the intra-specific and inter-specific competition among predators. A Caputo fractional-order derivative is utilized to include the influence of the memory on the constructed mathematical model. The mathematical validity is ensured by showing the model always has a unique, non-negative and bounded solution. Four kinds of equilibria are well identified which represent the condition when all populations are extinct, both two predators are extinct, only the first predator is extinct, only the second predator is extinct, and all populations are extinct. The Matignon condition is given to identify the dynamics around equilibrium points. The Lyapunov direct method, the Lyapunov function, and the generalized LaSalle invariant principle are also provided to show the global stability condition of the model. To explore the dynamics of the model more deeply, we utilize the predictor–corrector numerical scheme. Numerically, we find the forward bifurcation and the bistability conditions by showing the bifurcation diagram, phase portraits, and the time series. The biological interpretation of the analytical and numerical results is described explicitly when an interesting phenomenon occurs.
Hepatobiliary and Pancreatic Neoplasms: Essential Predictive Imaging Features for Personalized Therapy
Authors: Irizato Mariko , Minamiguchi Kiyoyuki , Uchiyama Tomoko , Kunichika Hideki , Tachiiri Tetsuya , Taiji Ryosuke , Kitao Azusa , Marugami Nagaaki , Inaba Yoshitaka , Tanaka Toshihiro
Published: February 2025

Predictive radiologic imaging combined with a clear understanding of tumor biologic characteristics aids in tailoring treatment plans for individuals with hepatobiliary and pancreatic neoplasms, and radiologists must grasp the implications of these findings to improve patient outcomes.
In Situ Monitoring Techniques and Analysis Theory for Electrochemical Corrosion in Subcritical and Supercritical Aqueous Systems
Authors: Bai Zhouyang , Li Yanhui , Xing Limei , Gao Pengfei , Yu Zhihong , Ding Shaoming , Macdonald Digby D. , Wang Shuzhong
Published: February 2025

Subcritical and supercritical water systems, critical in nuclear energy, thermal power, and pollutant treatment, operate under extreme conditions that intensify material corrosion. In-situ electrochemical monitoring provides essential real-time data for optimizing operational conditions and enhancing corrosion prediction models. This paper evaluates high-temperature electrochemical monitoring techniques, focusing on the performance of reference electrodes and research platforms. While hydrogen electrodes offer precision, their operational complexity limits practicality. Metal/metal oxide electrodes provide robustness but suffer from potential instability, and yttria-stabilized zirconia electrodes, though suitable for high temperatures, are fragile and challenging to fabricate. External pressure-balanced reference electrodes and flow-through versions represent promising alternatives but require further refinement, particularly in thermal liquid junction potential calibration. The development of advanced research platforms, facilitating in situ electrochemical testing via techniques like electrochemical impedance spectroscopy and potentiodynamic polarization curves, is also discussed. The point defect model and its supercritical water adaptation provide a robust framework for understanding corrosion mechanisms at the microstructural level. Ongoing innovation in electrode design, platform scalability, and diagnostic techniques will be essential to advancing corrosion monitoring in extreme environments, ensuring enhanced material performance and operational safety.
S, N co-doped Porous Carbon for the Effective Capacitive Deionization of Pb2+, Cd2+ and Cu2+ Ions: Performance and Mechanism
Authors: Xu Chaonan , Su Qin , Wang Rui , Liu Yuang , Zhao Donglin
Published: February 2025

In this work, the soybean-derived S, N co-doped porous carbon (SNPC) was successfully synthesized via thiourea modification and KOH activation. The SNPC has a large specific surface area (2310.642 m2·g−1), low resistance, excellent electron transfer capability and electric double layer capacitance (EDLC). The removal capacity of Pb2+, Cd2+ and Cu2+ by SNPC was 55.3, 48.0, and 5.3 mg·g−1 in 100 mg·l−1 signal solution at an operating voltage of 1.2 V, respectively. After undergoing 1000 charge and discharge cycles, the coulombic efficiency of SNPC remained consistently stable. Furthermore, even after subjecting it to 20 electro-sorption desorption experiments in a mixed solution, the removal efficiency for Pb2+, Cd2+ and Cu2+ still maintained a remarkable level of 90%. These results indicate that SNPC exhibits excellent cyclic performance and regeneration capability. The experimental results and DFT calculations demonstrate that the removal efficiencies of Pb2+, Cd2+ and Cu2+ by SNPC doped with S and N are superior to those by NPC solely doped with N. Thus, the present study offers an efficient and cost-effective material for the electro-sorption of Pb2+, Cd2+ and Cu2+.
The Promoting Effects of Fermented Bile Acid on Growth Performance and Intestinal Health of Litopenaeus vannamei Through the Modulation of Lipid Metabolism and Gut Microbiota
Authors: Guo Qing , Ma Haili , Zhao Lu , Liu Wenwen , Zhao Houfa , Liu Zuyue , Mu Cuimin , Wang Xuepeng
Published: February 2025

Two types of bile acids (BAs), named fermented bile acids (FBAs) and 170HDa, were produced by a biological approach to assess their effects on growth performance, metabolism, and intestinal health in white shrimp. In this study, five experimental diets were prepared with varying levels of FBAs (0.02% for A1, 0.03% for A2, 0.04% for A3, 0.05% for A4, and 0.06% for A5) and five diets containing different concentrations of 170HDa (0.02% for B1, 0.03% for B2, 0.04% for B3, 0.05% for B4, and 0.06% for B5). Additionally, positive diets (P) with commercial BAs at a level of 0.04%, along with and negative diet (N) without BA supplementation, were included as controls. FBAs and 170HDa were found to improve the growth performance including final weight, weight gain, and specific growth rate and reduce the activities of aspartate aminotransferase and alanine aminotransferase in hemolymph. The alkaline phosphatase (AKP) activity of hemolymph in shrimp treated with FBAs was generally higher than in groups treated with 170HDa and the control groups. However, the acid phosphatase (ACP) activity of hemolymph in shrimp treated with FBAs showed similar levels to those treated with 170HDa and the control groups. The gene expression levels of antilipopolysaccharride factor (ALF) and proPO were significantly lower in most FBAs and 170HDa‐treated groups compared to the control groups (p < 0.05). Additionally, the gene expression levels of α2M in hepatopancreas were significantly higher in the 170HDa‐treated groups compared to those in the FBAs‐treated group (p < 0.05). The FBAs and 170HDa significantly enhanced the intestinal health by boosting the proinflammatory capacity and increasing the diversity of the intestinal microbiota, thereby combating pathogenic microorganisms. Notably, there was a significant increase (p < 0.05) in the abundance of Proteobacteria, Firmicutes, Tenericutes, and Cyanobacteria at the phylum level, as well as Vibrio, Rhodobacter, and Shewanella at the genus level, respectively. These findings indicate that dietary FBAs and 170HDa have positive effects on growth performance and intestinal health by modulating lipid metabolic profiles, immune responses, the integrity of intestinal wall, and the diversity of intestinal microbes in white shrimp. This study suggests that FBAs and 170HDa could serve as effective dietary supplements to enhance shrimp production and health management in aquaculture, providing a promising strategy for sustainable aquaculture practices.
Growth Performance, Dietary Enzyme Profiling, and Antioxidant‐Induced Immunity of Crassostrea belcheri Fed With Microalgal Diet
Authors: Acharjee Mahima Ranjan , Hossain Md. Saddam , Newase Subeda , Das Trina , Haque Mohammad Ekramul , Nur Sifatun , Afrin Sadia , Minhaz Tashrif Mahmud , Khatoon Helena
Published: February 2025

Diseases, overfishing, and habitat loss are constantly reducing oyster populations. Moreover, environmental pollution and natural disasters also hinder offshore shellfish cultivation. Therefore, filter‐feeding bivalves could be cultivated on land‐based system to maintain the culture condition and secure food safety. Four different diets including Chaetoceros gracilis (CG), Tetraselmis chuii (TC), mixture of CG and TC (CG/TC), and fresh seawater without feed (Con) were trialed for Crassostrea belcheri spat in this research. After 35 days of culture, the highest survival rate (SR), volume, and weight increment (WI) of oyster and improved water quality appeared in CG/TC. In the same manner, CG/TC diet exhibited greater lipase (LPS), pepsin (PES), catalase (CAT), alkaline phosphatase (AKP), and hydrogen peroxide (H2O2) activity. Conversely, amylase (AMS), acid phosphatase (ACP), superoxide dimutase (SOD), malondialdehyde (MDA), and lysozymes (LZMs) activities were substantially higher in CG diet compared to TC, CG/TC, and Con. No significant differences were observed among CG, TC, and CG/TC for antioxidant capacity (AOC). In this investigation, mixed algal diet had excellent results for the growth and development of oyster, whereas unialgal diet improved immunity and AOC to survive in unfavorable conditions. This observation help elucidates the knowledge on microalgal diet influenced immune modulation and health of marine bivalves in the scenario of land‐based farming.
Distal Nerve Transfer for Opponensplasty in the Setting of High Median Nerve Injury: A Case Series
Authors: Emamhadi Mohammadreza , Haghani Dogahe Mohammad , Emamhadi Amirreza
Published: February 2025

Background: High median nerve injury leads to an absence of thumb opposition and irreversible thenar atrophy. Currently, distal nerve transfer is a new option for opponensplasty. The superiority of nerve transfer over traditional tendon transfer is that in nerve transfer, all thenar muscles may be reinnervated and so thumb functions are fully achieved, while in tendon transfer, the goal is to reanimate the function of abductor pollicis brevis (APB). This study aims to describe the results of opponensplasty using distal nerve transfer.

Materials and Methods: This article analyses the results of opponensplasty using the transfer of abductor digiti minimi (ADM) branch of the ulnar nerve to the recurrent branch of the median nerve. Clinical outcomes were assessed by objectively evaluating APB strength, degree of thumb opposition, and thenar muscle bulk. APB strength and degree of thumb opposition measured by Medical Research Council (MRC) and Kapandji scoring systems, respectively.

Results: From 2016 to 2019, six patients with a mean age of 29.5 years (five males and one female) with high median nerve injury were considered for opponensplasty using nerve transfer. Clinical improvement, including APB strength regaining and thumb opposition, was achieved in all patients. Moreover, recovery of thenar atrophy was observed in five patients.

Conclusion: In high median‐nerve injury, early reconstructive intervention can prevent the thenar muscle atrophy and leads to prompt reinnervation and complete restoration of thenar function. ADM branch of the ulnar nerve is a superior donor for this purpose.
Therapeutic Drug Monitoring for Dose Optimization of Infliximab in Patients With Inflammatory Bowel Disease: An Analysis of Canadian Real‐World Data
Authors: Sealey David C. , Ho Kai Fai , Zhou Z. Christina , Clark Michael , Feagan Brian G. , Panaccione Remo , Steinhart A. Hillary , Bolshtyansky Elena , Williamson Martin , Afif Waqqas
Published: February 2025

Background: Although it is generally believed that infliximab dose optimization in patients with inflammatory bowel disease with low serum infliximab concentration at trough results in increased treatment persistence, empirical data to support this notion are lacking. This study evaluated the association of infliximab therapeutic drug monitoring (TDM) and TDM‐associated dose optimization with persistence in real‐world practice.

Methods: Data from adults with Crohn’s disease (CD) or ulcerative colitis (UC) who participated in a national patient support program (PSP) in Canada were analyzed. Participants who had a first TDM evaluation (with a recorded infliximab trough concentration) in the maintenance phase of treatment were assessed (excluding those who underwent prior dose optimization). Persistence was evaluated using time‐dependent Cox proportional hazards models.

Results: In the overall population of patients with CD or UC, TDM was not associated with longer persistence (n = 13,203). In patients with no prior dose optimization (n = 2729) who had a serum infliximab concentration of < 3 μg/mL, dose optimization within 9 weeks of TDM was associated with significantly longer persistence (HR: 0.36; 95% CI: 0.26, 0.50 for CD [n = 711] and HR: 0.30, 95% CI: 0.21, 0.43 for UC [n = 501]). Sensitivity analyses yielded similar results when using a threshold concentration of < 5 μg/mL. In an analysis excluding patients who received no further treatment after TDM, the association between dose optimization and longer persistence was not confirmed in patients with CD, and mostly confirmed in patients with UC at a threshold concentration of < 3 μg/mL.

Conclusion: TDM‐associated dose optimization in patients with UC with low serum infliximab concentrations was associated with longer persistence. This association was not confirmed in patients with CD. This study demonstrated that real‐world data from a PSP‐generated cohort can be evaluated to inform clinical practice and that this approach may be complementary to other types of cohort studies.
Virtual Team Management Framework: A Qualitative Study
Authors: El Idrissi Ali , Fourka Mohamed
Published: February 2025

Virtual teams (VTs) are groups of people who work interdependently towards a common goal across geographical, temporal, and organizational boundaries, using technology to communicate and collaborate. Business competition, globalization, and rapid advancement in information and communication technology have propelled the growing prevalence of VTs. In addition, virtual collaboration allows bringing diverse talents and perspectives regardless of their location. However, cultural diversity and the lack of social interaction between VT members reduce the opportunity for informal communication and can cause conflicts and mistrust. The aim of this work is to develop a framework to enhance VT performance opportunities and reduce its setbacks. Based upon a quantitatively validated conceptual model, we developed a “virtual team performance framework” named VTPerF. Consisting of seven principles and seven processes, this framework presents the mechanisms that can help professionals improve VT performance. This framework is validated through a qualitative approach using Delphi method.
Effect of Surface Treatment on Physical and Tensile Properties of Borassus Fruit Fibers
Authors: Boimau K. , Bale J. , Suardana N. P. G. , Lokantara I. P. , Adoe D. G. H. , Pell Y.
Published: February 2025

The physical and tensile analysis of NaOH‐treated Borassus fibers is carried out to expand and open up further possibilities for their use as reinforcement in composites. This study will describe the NaOH treatment procedure on fibers utilizing the batch reactor approach, as the soaking method is typically used in this case. The physical analysis shows that the wettability behavior effect of NaOH was led by using 5%, 10%, and 15% of NaOH solutions. The result of each treatment using NaOH was then characterized by using SEM‐EDX to show the morphology and mechanical properties of the material before and after alkali treatment. Otherwise, FTIR was used to identify the functional group before and after alkali treatment. The tensile properties of the Borassus fibers (raw and treated) were also discussed. The wettability test was carried out by using the contact angle measurement technique. All samples (natural and treated fibers) showed a contact angle of less than 90°, which is associated with the hydrophilic surface properties. This result indicated that this treated fiber has polar properties. The increase of NaOH percentage in the treated samples caused a decrease in the contact angle. This phenomenon was related to the SEM image of natural and treated fibers. SEM result shows the cleaning effect of nonpolar components from the fiber surface and the roughening of the surface during the NaOH treatment. The result of FTIR indicates that several functional group components, such as impurities, lignin, and hemicellulose, were leaching out from row fibers after alkali treatment. The mechanical test results indicate improved tensile properties due to the NaOH treatment. Moreover, the samples from the fiber treated with 10% NaOH indicated the highest tensile strength.
Nursing Students’ Perspectives Toward Providing Oral Health Care for Older People
Authors: Veerasamy Arthi , Lyons Karl , Crabtree Ian , Ratnayake Jithendra , Brunton Paul
Published: February 2025

Introduction: Oral care for older adults in residential homes and long‐term hospital care has been largely ignored by health care professionals. The purpose of this study was to understand the perspectives of nursing students’ on incorporating oral health care topics in the nursing curricula.

Methods: As a part of a broader cross‐sectional quantitative study, we asked nursing students their opinions on incorporating an oral health care curriculum in their nursing programme. This resulted in a total of 148 responses, representing ~15% of New Zealand’s nursing graduates and exceeding the anticipated survey response rate of 10%. The respondents were from 61% of nursing schools across the country. Thematic coding was used to analyze and report on the participants’ responses.

Results: The nursing students thought oral health care education is overlooked in the nursing curriculum. Nursing students believed health professionals should be accountable for their patients’ oral health.

Conclusion: Nursing students do not feel confident providing oral care for their patients. However, they recognize their responsibility and understand the need to provide oral health care as part of long‐term care for older adults.
Flavor Quality Characterization of Rapeseed Oil During Storage by Physicochemical Analysis, Sensory Evaluation, Electronic Nose, and GC–O
Authors: Zhang Xiumin , Du Wenfeng , Zhang Danni , Zhang Na , Liu Yuan , Jiang Shui
Published: February 2025

Background: Rapeseed oil is the most widely consumed vegetable oil globally. However, the key aroma‐active compounds of rapeseed oil and their changes during storage are unclear. In this study, the flavor of rapeseed oil during storage was characterized by physicochemical analysis, sensory evaluation, electronic nose (E‐nose), and gas chromatography–olfactometry (GC–O).

Results: Peroxide value, acid value, and anisidine value gradually increased, while polyphenol content, tocopherol content, and sterol content showed a downward trend during storage. The E‐nose combined with the linear discriminate analysis (LDA) method could discriminate rapeseed oil with different storage times. The sensory attributes changed significantly from distinctive pickled aroma to rancid, green, and fried aromas during storage. This work provides the aroma‐active markers based on GC–O–MS for the quality evaluation of rapeseed oil during storage. A total of 136 volatile compounds were detected by GC–MS, and 16 odorants were identified by GC–O combined with aroma extract dilution analysis (AEDA). Finally, seven aroma‐active volatile compounds (3‐butenyl isothiocyanate, 2(5H)‐furanone, 2‐methoxy‐4‐vinylphenol, (E)‐2‐octenal, (E,E)‐2,4‐heptadienal, (E,E)‐2,4‐decadienal, and 3‐methyl‐pentanoic acid) with the odor activity values (OAVs) greater than 1 were identified as potential key aroma volatiles that contributed significantly to the overall aroma of rapeseed oil.

Conclusion: This study provided a comprehensive method to monitor the flavor quality change of rapeseed oil during storage. The identified volatile compounds could be the markers to characterize the quality changes of rapeseed oil during storage.
Comparison Between Radiographic Vertebral Left Atrial Size (VLAS) and Echocardiographic Methods for Predicting Left Atrial Remodeling in Dogs With Mitral Valve Disease
Authors: Pereira Natália Babolim , Bispo Guilherme Andraus , Carneiro Maurício Peres , Buzo Ricardo de Souza , Jané Daniela Ribas , Linhares Laís Calazans Menescal , Santos Paulo Sergio Patto dos , Pinoti Luciana Del Rio , Ferreira Wagner Luis
Published: February 2025

Objective: To investigate whether there is a positive correlation between vertebral left atrial size (VLAS) and echocardiographic variables—left atrium‐to‐aorta ratio (LA/Ao), left ventricular fractional shortening (FS), left ventricular internal diameter in diastole normalized to body weight (LVIDdn), and left ventricular early filling velocity (E‐wave)—and the Mitral INsufficiency Echocardiographic (MINE) echocardiographic score.

Study Design: Prospective randomized blinded study.

Animals: A total of 26 dogs.

Methods: Dogs diagnosed with MVD participated in the study. All patients underwent radiographic and echocardiographic evaluation.

Results: A high correlation strength could be observed between the left atrial enlargement predictor VLAS and the LA/Ao ratio (88%), as well as with LVIDdn (75%) and E‐wave (74%). Furthermore, a correction strength of 84% was verified between VLAS and the MINE score. The analysis of the FS variable did not demonstrate a statistically significant relationship (p value of 0.06).

Conclusions and Clinics Relevance: VLAS has a positive relationship and important correlation with the echocardiographic variables and MINE score. Associated with its higher accessibility in clinical routine is a potential diagnostic method to detect left atrial enlargement in dogs, contributing to the diagnostic conduct in DVM.
Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
Authors: Martins João , Penim Rute , Dos-Santos José , Fonseca Joana , Faísca Pedro , Martins Liege , Leal Nuno , Catarino José , Cabral Patrícia C. , Campos Sónia
Published: February 2025

This study aimed to determine the agreement between margin status in feline mammary tumors’ surgery by comparing 3 margin assessment techniques and to determine the predictive value of prognostic clinical and histological parameters for margin status using intraoperative margin assessment techniques. During the intraoperative period, 69 surgical margins from 12 female cats undergoing unilateral radical mastectomy were assessed by imprint cytology (IC) and scrape cytology (SC), both intraoperative cytological techniques. The excised mastectomy specimens were then evaluated postoperatively by histopathology (HP). Cochran’s Q test was used to determine differences in positive margin detection for the three methods. The agreement between the three methods was assessed using the Fleiss kappa coefficient. A binary logistic regression model was used to assess whether clinical and histological prognostic parameters could predict intraoperative margin status. There was no significant difference in positive margins between the three methods (p = 0.174). The agreement between the three methods was poor (Fleiss kappa = −0.020; 95% CI −0.156 to 0.117). Lymphovascular invasion is a significant predictor of a positive intraoperative margin, with an OD of 18.652 (95% CI = 1.742–199.652). These findings suggest that IC and SC are viable methods for assessing the status of surgical margins during surgery for feline mammary tumors. Intraoperative cytology may be complementary to HP, particularly in cases of lymphovascular invasion.
Supplementary table 6 from Mammographic Density in Relation to Breast Cancer Risk Factors among Chinese Women
Authors: Koka Hela , Tian Yuan , Deng Lu , Yu Kai , Li Er-Ni , Guo Changyuan , Guida Jennifer L. , Sung Hyuna , Chan Ariane , Hu Nan , Lu Ning , Gierach Gretchen L. , Li Jing , Yang Xiaohong R.
Published: February 2025

Heterogeneity in associations between mammographic density phenotypes with breast cancer risk factors by body mass index (BMI) and menopausal status.
Supplementary table 5 from Mammographic Density in Relation to Breast Cancer Risk Factors among Chinese Women
Authors: Koka Hela , Tian Yuan , Deng Lu , Yu Kai , Li Er-Ni , Guo Changyuan , Guida Jennifer L. , Sung Hyuna , Chan Ariane , Hu Nan , Lu Ning , Gierach Gretchen L. , Li Jing , Yang Xiaohong R.
Published: February 2025

Associations between percent dense (PDV), absolute dense (ADV), and non-dense (NDV) volumes with breast cancer risk factors when stratified by body mass index (BMI) and menopausal status.
Supplementary Table 5 from Adverse Birth and Obstetric Outcomes in the Offspring of Male Adolescent and Young Adult Cancer Survivors: A Nationwide Population-Based Study
Authors: Kao Wei-Heng , Chuang Yi-Fang , Huang Yi-Wei , Chen Po-Jui , Liu Yu-Cheng , Wang Chun-Chieh , Hsu Jun-Te , Shueng Pei-Wei , Kuo Chang-Fu
Published: February 2025

Supplementary Table 5: Outcomes observed in the offspring born to fathers with and without a positive history of cancer during adolescence or young adulthood, with the former group categorized according to the interval from diagnosis to delivery (<2 years versus ≥2 years)
Supplementary Table 4 from Adverse Birth and Obstetric Outcomes in the Offspring of Male Adolescent and Young Adult Cancer Survivors: A Nationwide Population-Based Study
Authors: Kao Wei-Heng , Chuang Yi-Fang , Huang Yi-Wei , Chen Po-Jui , Liu Yu-Cheng , Wang Chun-Chieh , Hsu Jun-Te , Shueng Pei-Wei , Kuo Chang-Fu
Published: February 2025

Supplementary Table 4: Outcomes observed in the offspring born to fathers with and without a positive history of cancer during adolescence or young adulthood, with the former group categorized according to age at diagnosis (15–29 years versus 30–39 years)
Supplementary table 2 from Mammographic Density in Relation to Breast Cancer Risk Factors among Chinese Women
Authors: Koka Hela , Tian Yuan , Deng Lu , Yu Kai , Li Er-Ni , Guo Changyuan , Guida Jennifer L. , Sung Hyuna , Chan Ariane , Hu Nan , Lu Ning , Gierach Gretchen L. , Li Jing , Yang Xiaohong R.
Published: February 2025

Associations between breast cancer risk factors with absolute dense (ADV) and percent dense (PDV) volumes, when stratified by tertiles of non-dense volume.
Supplementary table 1 from Mammographic Density in Relation to Breast Cancer Risk Factors among Chinese Women
Authors: Koka Hela , Tian Yuan , Deng Lu , Yu Kai , Li Er-Ni , Guo Changyuan , Guida Jennifer L. , Sung Hyuna , Chan Ariane , Hu Nan , Lu Ning , Gierach Gretchen L. , Li Jing , Yang Xiaohong R.
Published: February 2025

Results from testing interactions between BI-RADS categories and each risk factor in terms of density phenotypes.
Supplemental Table S9 from Evaluation of Multiple Breast Cancer Polygenic Risk Score Panels in Women of Latin American Heritage
Authors: Huang Xiaosong , Lott Paul C. , Hu Donglei , Zavala Valentina A. , Jamal Zoeb N. , Vidaurre Tatiana , Casavilca-Zambrano Sandro , Navarro Vásquez Jeannie , Castañeda Carlos A. , Valencia Guillermo , Morante Zaida , Calderón Mónica , Abugattas Julio E. , Fuentes Hugo A. , Liendo-Picoaga Ruddy , Cotrina Jose M. , Neciosup Silvia P. , Rioja Viera Patricia , Salinas Luis A. , Galvez-Nino Marco , Huntsman Scott , Sanchez Sixto E. , Williams Michelle A. , Gelaye Bizu , Estrada-Florez Ana P. , Polanco-Echeverry Guadalupe , Echeverry Magdalena , Velez Alejandro , Carmona-Valencia Jenny A. , Bohorquez-Lozano Mabel E. , Torres Javier , Cruz Miguel , Ho Weang-Kee , Teo Soo Hwang , Tai Mei Chee , John Esther M. , Haiman Christopher A. , Conti David V. , Chen Fei , Torres-Mejía Gabriela , Kushi Lawrence H. , Neuhausen Susan L. , Ziv Elad , Carvajal-Carmona Luis G. , Fejerman Laura
Published: February 2025

Supplemental Table S9 shows variants in European or Asian PRS panels with high linkage disequilibrium (LD) with rs851980
Supplementary Table 2 from Patterns of Subsequent Cancer Incidence Over Time in Patients with Breast Cancer
Authors: Deng Zhengyi , Visvanathan Kala
Published: February 2025

Supplementary Table 2. Risk of subsequent primary cancers among breast cancer survivors overall.
Supplementary Table 1 from Patterns of Subsequent Cancer Incidence Over Time in Patients with Breast Cancer
Authors: Deng Zhengyi , Visvanathan Kala
Published: February 2025

Supplementary Table 1. Baseline characteristics of breast cancer survivors.
Supplementary Table S4 from Timeliness of Lung Cancer Care and Area-Level Determinants in Victoria: A Bayesian Spatiotemporal Analysis
Authors: Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse , Heritier Stephane , Stirling Rob G. , Earnest Arul
Published: February 2025

R-hat or Potential Scale Reduction Factor (RSRF), Geweke diagnostic and effective Monte Carlo Sample size for assessing convergence of parameters for diagnostic delay
Supplementary Table S3 from Timeliness of Lung Cancer Care and Area-Level Determinants in Victoria: A Bayesian Spatiotemporal Analysis
Authors: Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse , Heritier Stephane , Stirling Rob G. , Earnest Arul
Published: February 2025

Model building process for diagnostic and treatment delay
Supplementary Figure S6 from Timeliness of Lung Cancer Care and Area-Level Determinants in Victoria: A Bayesian Spatiotemporal Analysis
Authors: Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse , Heritier Stephane , Stirling Rob G. , Earnest Arul
Published: February 2025

Histogram of smoothed relative risks for treatment delay
Supplementary Figure S4 from Timeliness of Lung Cancer Care and Area-Level Determinants in Victoria: A Bayesian Spatiotemporal Analysis
Authors: Tesema Getayeneh Antehunegn , Tessema Zemenu Tadesse , Heritier Stephane , Stirling Rob G. , Earnest Arul
Published: February 2025

Trace and density plot of model parameters to assess their convergences
Supplementary Table S3 from Incidence of Serious Complications following Screening Colonoscopy in Adults Ages 76 to 85 Years
Authors: Chubak Jessica , Ichikawa Laura E. , Merchant Sophie A. , Dalmat Ronit R. , Ziebell Rebecca A. , Jensen Christopher D. , Lee Jeffrey K. , Corley Douglas A. , Ghai Nirupa R. , Green Beverly B. , Skinner Celette Sugg , Schottinger Joanne E. , Breslau Erica S. , Levin Theodore R.
Published: February 2025

Supplementary Table S3: Cumulative incidence (95% Confidence Intervals) for broad analysis by follow-up time relative to screening colonoscopy
Supplementary Table S10 from Polygenic Risk Score, Healthy Lifestyle Score, and Colorectal Cancer Risk: A Prospective Cohort Study
Authors: Shen Yuefan , Chen Weiwei , Fu Chengqu , Liu Xinyi , Miao Junyan , Li Jiacong , Li Ni , Hang Dong
Published: February 2025

Association of genetics and lifestyle with colorectal cancer risk after further adjustment for screening history
Supplementary Figure S6 from Polygenic Risk Score, Healthy Lifestyle Score, and Colorectal Cancer Risk: A Prospective Cohort Study
Authors: Shen Yuefan , Chen Weiwei , Fu Chengqu , Liu Xinyi , Miao Junyan , Li Jiacong , Li Ni , Hang Dong
Published: February 2025

Ten-year colorectal cancer absolute risk, according to lifestyle and genetic risk in the PLCO cohort. Abbreviations: PLCO, Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial; PRS, polygenic risk score.
Figure S4 from CYP2D6 Phenotype and Breast Cancer Outcomes: A Bias Analysis and Meta-Analysis
Authors: MacLehose Richard F. , Ahern Thomas P. , Collin Lindsay J. , Li Aixin , Lash Timothy L.
Published: February 2025

Supplementary Figure S4: Forest plot of PM vs NM metabolizers, including only studies with tabular data.
Supplementary Figure 9 from Oncogenic KRAS Mutations Confer a Unique Mechanotransduction Response to Peristalsis in Colorectal Cancer Cells
Authors: Clevenger Abigail J. , Collier Claudia A. , Gorley John Paul M. , Colijn Sarah , McFarlin Maygan K. , Solberg Spencer C. , Kopetz Scott , Stratman Amber N. , Raghavan Shreya A.
Published: February 2025

Supplementary Figure 9 shows direct comparisons of LGR5 expression for inhibition conditions for all cell types tested.
Supplementary Figure 4 from Oncogenic KRAS Mutations Confer a Unique Mechanotransduction Response to Peristalsis in Colorectal Cancer Cells
Authors: Clevenger Abigail J. , Collier Claudia A. , Gorley John Paul M. , Colijn Sarah , McFarlin Maygan K. , Solberg Spencer C. , Kopetz Scott , Stratman Amber N. , Raghavan Shreya A.
Published: February 2025

Supplementary Figure 4 shows the gating strategy used for LGR5 analysis in Figure 6B.