bluuebunny/crossref_metadata_2025 huggingface dataset:
CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effects
Authors: Dhumale Pratibha , Nielsen Jakob Vennike , Hansen Anne Cathrine Schmidt , Burton Mark , Beck Hans Christian , Jørgensen Mads Gustaf , Toyserkani Navid Mohamadpour , Haahr Martha Kirstine , Hansen Sabrina Toft , Lund Lars , Thomassen Mads , Sørensen Jens Ahm , Andersen Ditte Caroline , Jensen Charlotte Harken , Sheikh Søren P
Published: May 2023

Cellular heterogeneity represents a major challenge for regenerative treatment using freshly isolated Adipose Derived Regenerative Cells (ADRCs). Emerging data suggest superior efficacy of ADRCs as compared to the ex vivo expanded and more homogeneous ADRCs (=ASCs) for indications involving (micro)vascular deficiency, however, it remains unknown which ADRC cell subtypes account for the improvement.
Surprisingly, we found regarding erectile dysfunction (ED) that the number of injected CD31+ ADRCs correlated positively with erectile function 12 months after one bolus of autologous ADRCs. Comprehensive in vitro and ex vivo analyses confirmed superior pro-angiogenic and paracrine effects of human CD31+ enriched ADRCs compared to the corresponding CD31- and parent ADRCs. When CD31+, CD31- and ADRCs were co-cultured in aortic ring- and corpus cavernous tube formation assays, the CD31+ ADRCs induced significantly higher tube development. This effect was corroborated using conditioned medium (CM), while quantitative mass spectrometric analysis suggested that this is likely explained by secretory pro-angiogenic proteins including DKK3, ANGPT2, ANAX2 and VIM, all enriched in CD31+ADRC CM. Single-cell RNA sequencing showed that transcripts of the upregulated and secreted proteins were present in 9 endothelial ADRC subsets including endothelial progenitor cells in the heterogenous non-cultured ADRCs. Our data suggest that the vascular benefit of using ADRCs in regenerative medicine is dictated by CD31+ ADRCs.
Quantitative evaluation of smart bed comfort in different typical conditions during resting.
Authors: Bai Xiangtian , Zhong Ming , Liu Yonghong , Hu Yafan , Ma Jun
Published: May 2023

In response to the general inability of smart beds to meet the health needs of chronic disease management and long-term care, this study proposed to investigate smart bed comfort in different typical conditions. The objective body pressure distribution and subjective comfort of participants were collected when the smart bed was in typical conditions. Pressure sensor mat was used to accurately measure pressure distribution. Participants reported in real time on the subjective comfort for each typical condition. Statistical data analysis intended to investigate changes in overall and local comfort when the smart bed is in different typical conditions, the correlation between perceived comfort and body pressure distribution. During the experiment, mean and peak pressures increased by up to 87.19% and 92.85%, respectively, owing to changes in smart bed conditions. However, major body pressure indicators correlated with the overall and local comfort of each area were not completely aligned. Statistical data analysis shows the im-portance of evening out forces and reducing peak pressures. Apart from mean pressure, peak pressure, particularly at the buttock, thigh and shank areas, is the key factor in comfort evaluation. Moreover, adequate bed board partitioning and linkage mechanism between adjacent boards better fit the body curve and enhance comfort. Accordingly, ensuring relatively balanced body forces and avoiding the feeling of weightlessness improve user comfort and health significantly. The succeeding study will further investigate the relationship between smart bed comfort and changes in free lying positions and moving activities, hoping to provide a comfort studies basis for long-term care.
TEAD4 antagonizes cellular senescence by remodeling chromatin accessibility at enhancer regions
Authors: Zhang Donghui , Zhu Yanmei , Ju Yanmin , Zou Xiaopeng , Zhang Hongyong , Guan Yiting
Published: May 2023

Dramatic alterations in epigenetic landscapes are known to impact genome accessibility and transcription. Extensive evidence demonstrates that senescent cells undergo significant changes in chromatin structure; however, the mechanisms underlying the crosstalk between epigenetic parameters and gene expression profiles have not been fully elucidated. In the present study, we delineate the genome-wide redistribution of accessible chromatin regions that lead to broad transcriptome effects during senescence. We report that the distinct senescence-activated accessibility regions (SAAs) are always distributed in H3K27ac-occupied enhancer regions, where the SAAs are responsible for elevated flanking SASP expression and aberrant cellular signaling relevant to SASP secretion. Mechanistically, a single transcription factor, TEAD4, moves away from H3K27ac-labled SAAs to allow for prominent chromatin accessibility reconstruction during senescence. The enhanced SAA signal driven by TEAD4 suppression subsequently induces a robust increase in the expression of adjacent SASP genes and the secretion of downstream factors, which contribute to the progression of senescence. Our findings illustrate a dynamic landscape of chromatin accessibility following senescence entry, and further reveal an insightful function for TEAD4 in regulating the broad chromatin state that modulates the overall transcriptional program of SASP genes.
The Value of Rockall and Forrest Classification in the Prognosis of Patients With Gastrointestinal Bleeding Applying to the Emergency Department
Authors: TUNCAR Alpay , CANBAZ Hayri , KIRIŞ Tolga , ÇOLAK Tamer , ALAY Mustafa Tarık , ÇAKIR Adem
Published: May 2023

Purpose
Gastrointestinal system bleeding (GIB) is a common and highly morbid condition with high mortality rates frequently observed in emergency departments. Several scoring systems have been used to predict mortality and rebleeding in these cases. Among these scoring systems, Rockall Score (RS) and Forrest classification (FS) are commonly used. In our study, we aimed to evaluate the predictive power of RS for mortality, rebleeding, and the need for blood transfusion, and to provide benefits to clinicians.
Methods
The study was designed as a retrospective and single-center study, conducted between January 1, 2017, and January 1, 2018. Demographic data, RS and FS scores, clinical outcomes (mortality, rebleeding, blood transfusion requirement, and final outcomes) of the cases were evaluated.
Results
Of the 109 patients in our study, 60.6% were male, and the mean age was 67.83 ± 16.75 years. The most common presenting complaint was black stool, and the most frequently detected finding was melena. The most common endoscopic diagnosis was erosive gastritis (24.8%). The use of acetylsalicylic acid and anticoagulants was significantly higher in cases of lower gastrointestinal bleeding. There was a negative correlation between the RS score and hemoglobin level of the patients (r=-0.537; p < 0.001), while a positive linear correlation was observed between RS and mortality (r = 0.352; p < 0.001), rebleeding (r = 0.381; p < 0.001), and blood transfusion requirement (r = 0.489; p < 0.001). Additionally, there was a positive linear correlation between the amount of blood product given to the patient and RS (r = 0.432; p < 0.001). The discriminatory power of RS for predicting mortality, rebleeding, and blood transfusion was evaluated, and our results showed an AUC of 0.802 (95% CI = 0.684–0.921; p < 0.001) for mortality, an AUC of 0.764 (95% CI = 0.641–0.886; p < 0.001) for rebleeding, and an AUC of 0.815 (95% CI = 0.731–0.899; p < 0.001) for blood transfusion requirement.
Conclusions
RS provides risk stratification in cases of GIB and aids in early treatment and prognosis prediction for high-risk patients. Furthermore, it has a high predictive power in determining rebleeding, mortality, and the need for blood transfusion. We believe that RS can be beneficial for clinicians in the management, prognosis, and mortality risk assessment of GIB cases, particularly in clinics such as emergency departments where a large number of patients need to be evaluated and treatment and discharge decisions are made within a short period of time.
Enhancing Accessibility in Retinal Imaging through the Universal Smartphone Adapter
Authors: Latip Aisya Amelia Abdul , Kipli Kuryati , Kamaruddin Abang Mohammad Nizam Abang , Sapawi Rohana , Lias Kasumawati , Jalil Muhammad Arif , Tamrin Khairul Fikri , Tajudin Nurul Mirza Afiqah , Ong Han Yi , Mahmood Muhammad Hamdi , Jali Suriati Khartini , Sahari Siti Kudnie , Mat Dayang Azra Awang , Lim Lik Thai
Published: May 2023

Background
The revolutionary technology of smartphone-based retinal imaging has been consistently improving over the years. Smartphone-based retinal image acquisition devices are designed to be portable, and easy to use, besides being low-cost which enables eye care to be more widely accessible especially in geographically remote areas. This enables early disease detection for those who are in low- and middle- income population or just in general has very limited access to eye care. This study investigates the limitation of smartphone compatibility of existing smartphone-based retinal image acquisition devices. Additionally, this study aims to propose a universal adapter that is usable with an existing smartphone-based retinal image acquisition device, the PanOptic ophthalmoscope. This study also aims to simulate the reliability, validity, and performance overall of improved develop prototype. Existing studies have shown that the concept of smartphone-based retinal imaging is still limited to screening purposes only. Furthermore, existing smartphone-based devices also have a limited smartphone compatibility where it is only usable with specific smartphone models.
Methods
A literature review was conducted that identifies the limitation of smartphone compatibility among existing smartphone-based retinal image acquisition devices. Designing and modelling of proposed adapter was performed using the software AutoCAD 3D. For proposed performance evaluation, finite element analysis (FEA) in the software Autodesk Inventor and 5-point scale method were applied.
Results
It was identified how a universal adapter is beneficial in broadening the usability of existing smartphone-based retinal image acquisition devices as most of the devices that are available in the market have limited smartphone compatibility. A functional universal adapter was developed and found to be suitable with two smartphones that have different camera placement and dimensions. The proposed performance evaluation method was able to generate efficient stress analysis of the proposed adapter design.
Conclusion
The concept of a universal and suitable adapter for retinal imaging using the PanOptic ophthalmoscope was presented in this paper. Performance evaluation methods proposed were identified to be sufficient to analyze the behavior of proposed adapter when an external load is applied and determine its suitability with the PanOptic ophthalmoscope.
A double negative cascade mediated by epigenetic modifications of EFD (Exine Formation Defect) to HB21 coordinates anther development in plant
Authors: Zhang Cheng , Xiong Ao-Tong , Ren Meng-Yi , Zhao Yanyun , Huang Min-Jia , Zhang Zheng , Wang Yun , Zheng Quan-Quan , Yang Zhong-Nan
Published: May 2023

Epigenetic modifications are essential for plant development. EFD encodes a SAM-dependent methyltransferase essential for the pollen wall pattern formation and male fertility in Arabidopsis. Here, we found that the expression of DRM2, a de novo DNA methyltransferase in plants, can complement the defects of efd, suggesting its de novo DNA methyltransferase ability. Genetic analysis reveals that EFD plays roles through HB21 as its knockout fully restored the efd fertility. The methylation and H3K27me3 analysis indicates that EFD represses the transcription expression of HB21 through epigenetic modifications. Besides, both EMSA and tobacco transient expression assays showed that HB21 repressed the expression of the genes including CalS5, RPG1/SWEET8, CYP703A2 and NST2 which are essential for pollen formation and anther dehiscence. Altogether, our study reveals a double negative regulatory cascade mediated by epigenetic modifications to coordinate anther development, which provides new insights into epigenetic regulation and anther development.
Acceptability of STEPPS for outpatient adolescents in the United States: A case study
Authors: Cano Kiana , Balzen Kennedy M. , Vanwoerden Salome , Penner Francesca , Sharp Carla
Published: May 2023

Background. Borderline personality disorder (BPD) is a severe mental illness that typically onsets during adolescence. Emotion dysregulation is a key feature of BPD, and several interventions have been developed to target these difficulties. However, many barriers to care exist for adolescents with BPD, such as difficult-to-find and time-consuming treatment. Considering these limitations, the evidence-based group treatment Systems Training for Emotional Predictability and Problem Solving (STEPPS) was previously adapted for adolescents with BPD or symptoms of BPD and has shown promise in a preliminary study, but more work is needed examining STEPPS for use in adolescents.
Methods. This paper uses a case study approach to evaluate STEPPS in two adolescents and their parents who completed 18 weeks of STEPPS intervention. Adolescents and their parents completed the STEPPS assessment of borderline personality features weekly during treatment to track changes over time. Moreover, participants completed assessments of emotion regulation, interpersonal difficulties, and general psychopathology before treatment, after program completion, and at 6- and 12-month follow-ups.
Results. Results showed that both adolescents reported a decline in BPD features following STEPPS, albeit with unique patterns in decline. Parent reports of youth BPD features were less straightforward but suggested improvement in BPD features during treatment, but stability or small increases in symptoms at post-treatment and follow-up assessments. Results also illustrated improvements in emotion regulation, reduced stress in the parent-adolescent relationship, improvement in inadequate parent-adolescent boundaries, and a decline in functional impairment.
Conclusions. Overall, STEPPS may offer a brief and cost-effective treatment for BPD, although more research is needed to fully establish its efficacy in adolescents.
A Comparison of Film Analysis Software for Radiotherapy Patient-Specific Quality Assurance
Authors: Buddhavarapu Athreya
Published: May 2023

Aim:
The aim of this study was to investigate the suitability of three Radiochromic film analysis software programs for radiotherapy patient-specific quality assurance (PSQA): FilmQA Pro v5.0, SNC Patient v6.2, and eFilmQA v5.0.
Methods:
Film calibration was conducted for each software followed by three sets of measurements. The first set assessed calibration accuracy by comparing measured and delivered doses at increments different from those used for calibration. The second set used each software to conduct PSQA through gamma analysis on 10 Stereotactic Radiosurgery (SRS) and Stereotactic Body Radiation Therapy (SBRT) patients. The third set utilized SNC Patient and eFilmQA to carry out gamma analysis on a collection of 4 digital test images, eliminating delivery and scanning uncertainties from impacting the analysis. Key supporting features within each software for conducting gamma analysis were identified.
Results:
In general, FilmQA Pro and eFilmQA were deemed comparable and favoured over SNC Patient due to the presence of key features such as triple channel dosimetry, auto-optimization, and dose scaling. FilmQA Pro has a substantial user base and established reputation. eFilmQA, having been introduced more recently, serves as a viable alternative to FilmQA Pro, having been further refined for stereotactic radiotherapy PSQA.
Conclusion:
This study investigated the suitability of three film analysis software programs (FilmQA Pro, eFilmQA and SNC Patient) for radiotherapy PSQA. Results from the investigation indicated that both FilmQA Pro and eFilmQA were comparable in performance and preferred over SNC Patient.
Mediating role of self-compassion in the relationship between psychological flexibility and psychological well-being in women with breast cancer
Authors: Chen Haoting , Abdelrasheed Nasser Said Gomaa , Mecheser Abdullah Eiada , Tayyib Nahla A , Ramaiah Pushpamala , Prasad KDV , Estrada-Araoz Edwin Gustavo , Alkhayyat Safa , Ramadan Montather F. , Abdollahi Abbas
Published: May 2023

Background: Previous studies have investigated the relationship between psychological flexibility and psychological well-being among university students. In addition to examining the relationship between these two variables, this study enhances our understanding about the process underlying the relationship between psychological flexibility and psychological well-being among women with breast cancer. The aim of the present study was to test the mediating role of self-compassion in the relationship between psychological flexibility and psychological well-being among Iranian women with breast cancer.
Methods: The participants of this study, 220 women with breast cancer (ages 28 to 58 years, mean age: 42.16, SD: ±6.9.54) from three hospitals, in Tehran, Iran completed questionnaires about psychological flexibility, self-compassion and psychological well-being.
Results: The results from structural equation modeling indicated that psychological flexibility and self-compassion positively predicted psychological well-being and psychological inflexibility negatively predicted psychological well-being among breast cancer patients. The findings from mediation analysis demonstrated that self-compassion partially mediated the relationships between psychological flexibility and psychological inflexibility with psychological well-being. The present study brings to light an underlying mechanism of the relationships between psychological flexibility and psychological inflexibility with psychological well-being via the mediating variable of self-compassion for patients with breast cancer.
Reduced expression of the Htr2a, Grin1, and Bdnf genes and cognitive inflexibility in a model of high compulsive rats
Authors: Prados-Pardo Angeles , Martin-Gonzalez Elena , Mora Santiago , Martin Carlos , Olmedo-Cordoba Manuela , Perez-Fernandez Cristian , Sanchez-Santed Fernando , Moreno-Montoya Margarita
Published: May 2023

Compulsivity is a core symptom in different psychopathological disorders, characterized by excessive behaviors and behavioral inflexibility. In preclinical studies, the selection of high drinker (HD) versus low drinker (LD) rats by schedule-induced polydipsia (SIP) is a valid model of compulsivity. HD rats shows a compulsive phenotype with a reduction in serotonin 2A (5-HT2A) receptor binding levels in the frontal cortex (FC). However, is necessary further explore the cognitive components regarding inflexibility in the compulsive HD rats, as well as, the related brain gene expression. Here, first, we assessed spatial memory and cognitive inflexibility by Morris Water Maze (MWM), working and reference memory by Radial Arm Maze, and behavioral deficits in stimulus processing by novel object recognition test. Second, we analyed the genetic expression of 5HT2A, 5HT2C, glutamate NMDA receptors, and brain-derived neurotrophic factor (BDNF) in FC, hippocampus, and amygdala. HD rats confirmed a cognitive inflexibility profile in the reversal condition in MWM compared to LD rats, while no differences were observed in stimulus processing, spatial and working memory. Moreover, HD rats showed a reduced expression of the Htr2a,Grin1, and Bdnf genes in FC. Furthermore, there was a negative correlation between the relative expression of the Htr2a, Grin1 and Bdnf genes in FC and the level of compulsive water intake in HD rats on SIP. These data reveal that cognitive inflexibility may not be associated with memory or stimulus processing alterations, but may result from a region-specific alteration on FC of the gene expression Htr2a, Grin1, and Bdnf in compulsive individuals.
Evaluating the Effectiveness of Different Software Testing Frameworks on Software Quality
Authors: de Silva Dilshan , Samarasekara H.M.P.P.K.H , Ariyarathna W.K.K.H , de Silva L.S.M , de Silva W.L.P , Methmini P.K Hasara , Wijewickrama G.R.P.S , Madushika H.A.J
Published: May 2023

The effectiveness of software testing frameworks on software quality is an important area in software engineering. This paper evaluates the effectiveness of popular software testing frameworks, namely Selenium, TestNG, Cucumber, JMeter, and Cypress, on different software qualities, including maintainability, security, code quality, reliability, performance, and usability. The study includes a literature review of previous studies on the effectiveness of these testing frameworks and an empirical study that compares the performance of these frameworks on the selected software qualities using a set of benchmark applications. The results show that the effectiveness of a software testing framework on a particular software quality depends on the specific requirements of the project. For example, Selenium and Cypress are effective for testing user interface functionality and usability, while JMeter is effective for load and stress testing. Thus, software developers and testers should carefully evaluate the different testing frameworks in relation to the software quality requirements of their project before selecting a testing framework. The study concludes that software testing frameworks play a crucial role in ensuring software quality, and selecting the appropriate testing framework is important for achieving the desired software quality goals.
Disease Burden in Refugees in Mainland Grecian Refugee Camps, 2016-2017: A retrospective cross-sectional study
Authors: Scales Sarah Elizabeth , Park Jee Won , Nixon Rebecca , Guha-Sapir Debarati , Horney Jennifer A
Published: May 2023

Background.
Despite the importance of baseline health data for evidence-informed decision-making, these data are rarely available for displaced populations. At the height of the European refugee crisis, most of those seeking asylum in Europe were from regions with high prevalences of communicable and non-communicable diseases. To create an epidemiologic profile for refugees in mainland Grecian refugee camps, this study assessed the prevalence of 11 communicable and non-communicable diseases among refugees utilizing Médecins du Monde (MdM) in-camp clinics.
Methods.
The prevalences of selected diseases among individuals utilizing MdM services were determined from data collected at mainland Grecian refugee camps clinics from April 2016 - July 2017. Overall and age-specific prevalences were reported. Differences in disease burden among refugees from the largest sending countries - Afghanistan and Syria - were compared using prevalence ratios and 95% confidence intervals. Patterns in results were compared with disease burden estimates in sending countries and with findings from comparable settings.
Results.
Respiratory tract infections (RTIs) were the most prevalent outcome. Among RTIs, upper RTIs were most common, with a prevalence of nearly 40%; throughout the study period, over 46% of children under 18 years had at least one upper RTI consultation. Musculoskeletal conditions (3.64%), were the most prevalent non-communicable outcome, followed by hypertension (2.21%) and asthma (1.28%). Afghans were 31.68% more likely than Syrians to have a consultation for at least one condition (PR: 1.32; 95% CI: 1.25, 1.39). The prevalence of RTIs was comparable to sending countries, but there was a comparatively lower burden of other conditions among refugees than literature estimates from sending countries.
Conclusion.
Refugees utilizing MdM clinics in camps had higher burdens of communicable diseases - predominantly RTIs - relative to non-communicable diseases. Non-communicable disease burdens were comparatively lower than reported prevalences from in-country populations. These findings can be attributed to a range of considerations including differences in demographic profiles between sending countries and refugee populations and missed opportunities for utilizing clinical care. Based on these data, displaced populations have considerable health needs. Further investment is needed to capture the health profiles of displaced populations to support evidence-informed decision-making processes in humanitarian emergency responses.
Similarity matching of TCM prescription rational medication based on Siamese network
Authors: Xiong Wangping , Wang Kaiqi , Liu Shixiong , Liu Zhaoyang , Zhu Yimin , Liu Peng , Yang Ming , Zhou Xian
Published: May 2023

Background
Chinese medicine taking is an important link to ensure rational medicine use, and prescription matching can excavate more information on medicine use. traditional Chinese medicine (TCM) prescription data feature relationship is complex and multi-dimensional, and the prescription similarity matching model suffers from single attribute quantification, lack of elemental relationships, and the similarity of efficacy and primary function cannot be quantified, which brings challenges to the quantification of prescription similarity.
Methods
The focus of this study is to construct a framework for matching traditional Chinese medicine prescriptions with classic formulae, which integrates multiple features (MFTCMC) and explores the classic formulae contained in prescriptions to provide references for the medication-taking behavior of clinical traditional Chinese medicine prescriptions. Firstly, the prescription compatibility weight is calculated through Analytic Hierarchy Process (AHP), and the various dosage weights of herbs are integrated to construct a comprehensive weighting method, which selects a set of key herbs to form a core prescription. Secondly, the performance features of the core prescription are quantified by using the attribute labeling strategy of Chinese medicine properties. Finally, the relationship features of the core prescription are extracted by using a Bidirectional Long Short-Term Memory Network (BiLSTM), and the vector representation similarity matrix is constructed by combining the Siamese network framework. The matching score between the core prescription and the classic formula is calculated through a fully connected layer, and the traditional medication method of the matched classic formula is selected to provide references for the medication behavior of clinical prescriptions.
Results
Based on the prescription matching dataset constructed in this paper, the precision and F1 value reached 94.45% and 94.34% respectively, which is a significant improvement compared to several other models.
Conclusions
Compared with other models, the proposed MFTCMCF can effectively improve the performance of matching between prescriptions and classic formulae, and the results are more in line with the clinical practice of traditional Chinese medicine. This further improves the medication behavior of clinical prescriptions and provides new insights for research on recommended methods of taking traditional Chinese medicine prescriptions.
Psychometric Properties of the Wellbeing Literacy 6-item (Well-Lit 6) scale in Chinese Military Academy Cadets
Authors: Jia Zhen , Zheng Fangfang , Wang Feifei , Yang Guoyu
Published: May 2023

Background
This study aimed to examine the psychometric properties of the wellbeing literacy 6-item (Well-Lit 6) scale in Chinese military academy cadets.
Methods
A total of 3218 undergraduate students from five military academies in China were recruited to complete questionnaires online. The data set was analyzed through item analysis to examine the homogeneous reliability and split-half reliability of the Chinese version of Well-Lit 6 scale. Structural validity was tested through exploratory factor analysis and confirmatory factor analysis. Correlation analysis was then conducted to verify the correlational validity and convergent-discriminant validity of the calibration scale, and regression analysis to examine the incremental validity. Independent sample t-test and one-way ANOVA were also conducted to explore the characteristics of wellbeing literacy among Chinese military academy cadets.
Results
(1) The items of the scale showed high discrimination; (2) The alpha coefficient of the scale was 0.986 and the split-half reliability was 0.981, indicating high homogeneous reliability and split-half reliability; (3) The scale model fitted well and displayed structural validity; (4) The correlation between well-being literacy and related indicators was significant (P < 0.001), and the calibration correlation and convergent-discriminant validity of the scale were high; (5) After gradually adding demographic variables, known predictors factors and well-being literacy, the ∆R2 for subjective well-being, life satisfaction, depression, and anxiety ranged from 0.036 to 0.067, 0.184 to 0.340, and 0.009 to 0.017, respectively (all P < 0.001), showing high incremental validity; (6) the total well-being literacy scores differed significantly by gender (P < 0.05), grade (P < 0.001), and parenting style (P < 0.001) .
Conclusion
The Chinese version of the Well-Lit 6 is reliable and valid in predicting and accessing the subjective well-being, life satisfaction, emotion regulation and psychological resilience of Chinese military academy cadets.
Association between immune-related adverse events and survival in patients with renal cell carcinoma treated with nivolumab plus ipilimumab: Immortal time bias-corrected analysis.
Authors: Washino Satoshi , Shirotake Suguru , Takeshita Hideki , Inoue Masaharu , Miura Yuji , Hyodo Yoji , Kagawa Makoto , Izumi Keita , Oyama Masafumi , Kawakami Satoru , Saito Kazutaka , Matsuoka Yoh , Taniuchi Satsuki , Shintani Ayumi , Miyagawa Tomoaki
Published: May 2023

Background: Immune-related adverse events (irAEs) in patients treated with immune check inhibitors are associated with favourable response rate and survivals in multiple cancers, including renal cell carcinoma (RCC). The aim of this study was to investigate how irAEs were associated with improved survivals in advanced RCC patients treated with nivolumab plus ipilimumab.
Materials and methods: This retrospective study included patients who received nivolumab plus ipilimumab between September 2018 and February 2022 at six centres. We assessed associations of the development and the number of irAEs with overall survival (OS) and progression-free survival (PFS). To eliminate immortal time bias, landmark analysis and a Cox model with time-dependent variables were used.
Results: This study included 129 patients with a median follow-up of 12.3 months. The 2-year OS and PFS rates were 55% and 42%, respectively. Ninety six patients experienced irAEs. The development of irAEs was positively associated with OS and PFS rates (hazard ratio [HR] 0.328, 95% confidence interval [CI] 0.165–0.648, p = 0.001; HR 0.334, 95% CI 0.151–0.737, p = 0.007). Patients who experienced multiple irAEs had longer OS (HR 0.507, 95% CI 0.235–1.097, p = 0.085 or HR 0.245, 95% CI 0.110–0.544, p < 0.001) and PFS (HR 0.572, 95% CI 0.316–1.036, p = 0.085 or HR 0.267, 95% CI 0.113–0.628, p = 0.002) compared with those who experienced single or zero irAE.
Conclusions: Developing irAEs, particularly multiple irAEs, is associated with favourable survivals in advanced RCC patients treated with nivolumab plus ipilimumab.
Desynchronization of neuronal firing in multiparameter transcranial ultrasound stimulation
Authors: Bian Nannan , Long Ai , Yuan Yi
Published: May 2023

Low-intensity transcranial ultrasound stimulation, a novel neuromodulation technique, that possesses the advantages of non-invasiveness, high penetration depth, and high spatial resolution, has achieved positive neuromodulation effects in animal studies. But the regulatory mechanism remains controversial. The intramembrane cavitation effect is considered one of the mechanisms for ultrasound neuromodulation. In this study, the modified equations of ultrasonic cavitation bubble dynamics were coupled with the dual-coupled neuron Hindmarsh-Rose model, small-world neural network model, and the Jansen-Rit neural mass model, which simulate simple coupled neurons, complex neuronal networks, and discharge signals in epileptic disorders respectively. The results demonstrated that transcranial ultrasound stimulation has an appreciable modulatory effect on neuronal firing desynchronization in Hindmarsh-Rose model and small-world neural network model. The desynchronization effect is related to the stimulation frequency and intensity. Furthermore, ultrasound stimulation has an inhibitory effect on epileptic seizures, and the effect is enhanced by increasing ultrasound frequency from 0.1-1.0MHz. This is the first combination of ultrasonic intramembrane cavitation effect theory with neurons and neural network firing desynchronization, which can provide guidance of parametric and theories support for the studies of neurological diseases such as epilepsy and Parkinson's disease.
Loneliness and Suicide Mitigation for Students using GPT3-Enabled Chatbots
Authors: Maples Bethanie , Cerit Merve , Vishwanath Aditya , Pea Roy
Published: May 2023

Mental health is a crisis for learners globally, and digital support is increasingly seen as a critical resource. Concurrently, Intelligent Social Agents receive exponentially more engagement than other conversational systems, but their use in digital therapy provision is nascent. A survey of over 1000 student users of the Intelligent Social Agent, Replika, investigated participants’ loneliness, perceived social support, use patterns, and beliefs about Replika. We found participants were more lonely than typical student populations but still perceived high social support. Many used Replika in multiple, overlapping ways - as a friend, a therapist, and an intellectual mirror. Many also held overlapping and often conflicting beliefs about Replika - calling it a machine, an intelligence, and a human. Critically, 3% reported Replika halted their suicidal ideation. Comparative analysis of this group with the wider participant population is provided.
Study on chemical composition analysis and lipid metabolism regulation activity of different parts of Eucommia ulmoides
Authors: Gong Pin , Zhai Pengtao , Chen Xuefeng , Sun Wangyang , Wang Peipei , Guo Yuxi , Wang Zhineng , Li Nan , Zhai Wenjun , Zheng Benzhong
Published: May 2023

Eucommia ulmoides has lipid-lowering, antihypertensive, hypoglycemic and anti-aging effects. The main medicinal part of Eucommia ulmoides bark (EBE), but its slow growth and peeling easily lead to tree death, which limits the extensive use of Eucommia ulmoides resources and causes the lack of Eucommia ulmoides resources. It is not clear whether Eucommia ulmoides leaves (ELE), flowers (EFE) and seeds (ESE) can be used as substitutes for Eucommia ulmoides bark. In this study, UPLC-Q-Orbitrap-MS was used to analyze the active ingredients in the bark, leaves, flowers and seeds of Eucommia ulmoides. HPLC method was established for the determination of 8 common active ingredients in the bark, leaves, flowers and seeds of Eucommia ulmoides. The content of active ingredients is also based on the current research on Eucommia ulmoides, and the regulation activity of lipid metabolism was compared. The results showed that 119 compounds were identified from 12 classes of Eucommia ulmoides, and the skin and leaves of Eucommia ulmoides contained a lot of the same active ingredients. The content of turbinol diglucoside in Eucommia ulmoides bark was the highest, and the content of chlorogenic acid in Eucommia ulmoides leaves was the highest. After Eucommia intervention, all indexes of cell model were improved and lipid metabolism regulation ability was improved: EBE, ELE, EFE, ESE; EBE and ELE had similar lipid lowering effect. After Eucommia's treatment, mRNA expression of AMPK increased in a volume-dependent manner, while mRNA expression of SREBP-1c, FAS and ACC decreased in a volume-dependent manner. Therefore, Eucommia's improvement in steatosis of HepG2 cells may be related to the regulation of AMPK, SREGP-1C, FAS and ACC signaling pathways. In conclusion, it can be concluded that Eucommia ulmoides leaves can replace Eucommia ulmoides skins in the regulation of lipid metabolism and improve the comprehensive utilization of Eucommia ulmoides resources.
Shear strength characteristics of basalt fiber-reinforced loess: experimental investigation and model prediction
Authors: Chen Chong-kun , Li Gang , Liu Jia , Xi Yu , Nan Jing-jing
Published: May 2023

Loess owns the characteristics of collapsibility, disintegration and solubility, which pose a challenge to engineering construction. To examine the shear strength of basalt fiber-reinforced (BFR) loess, consolidated undrained (CU) triaxial tests were conducted to explore the impacts of water content (w), fiber length (FL), fiber content (FC) and cell pressure (σ3) on the shear strength. The microstructure of reinforced loess was constructed using SEM measurements to reveal the reinforcing mechanism of basalt fibers. According to the results, the shear strength model was established taken into account the impacts of FL, FC, and fiber diameter (d) using the regression analysis method. The results showed that the peak strength of BFR soils enhanced as FL, FC, and σ3 increasing, whereas it decreased with increasing of w. The reinforcing mechanism of fibers was divided into a single tensile effect and spatial mesh effect, and the bridging effect transformed the force, which improved the overall ability of composites to resist deformation and damage. The experimental and calculated results agreed well, which suggested the model is suitable for predicting the shear strength of BFR loess. The research results can offer a guideline for the application of BFR loess in the subgrade and slope engineering.
Impact of a positive end-expiratory pressure strategy on oxygenation, respiratory compliance, and hemodynamics during laparoscopic surgery in non-obese patients: a systematic review and meta-analysis
Authors: Arinova Gulfairus A. , Yukhnevich Yekaterina A. , Khamitova Zaukiya K. , Kim Sergey I. , Zhumabayev Murat B. , Berdiyarova Gulbanu S. , Shalekenov Sanzhar B. , Mukatova Irina Y. , Yaroshetskiy Andrey I.
Published: May 2023

Background.
Higher positive end-expiratory pressure (PEEP) during laparoscopic surgery may increase oxygenation and respiratory compliance. This meta-analysis aimed to compare the impact of different intraoperative PEEP strategies on arterial oxygenation, compliance, and hemodynamics during laparoscopic surgery in non-obese patients.
Methods.
We searched RCTs in PubMed, Cochrane Library, Web of Science, and Google Scholar from January 2012 to January 2022 comparing the different intraoperative PEEP (Low PEEP (LPEEP): 0 mbar; Moderate PEEP (MPEEP): 5–8 mbar; high PEEP (HPEEP): >8 mbar; individualized PEEP - iPEEP) on arterial oxygenation, respiratory compliance (Cdyn), mean arterial pressure (MAP), and heart rate (HR). We calculated mean differences (MD) with 95% confidence intervals (CI), and predictive intervals (PI) using random-effects models. The Cochrane Bias Risk Assessment Tool was applied.
Results.
21 RCTs (n = 1554) met the inclusion criteria. HPEEP vs LPEEP increased PaO2 (+ 29.38 [16.20; 42.56] mmHg, p < 0.0001) or PaO2/FiO2 (+ 36.7 [+ 2.23; +71.70] mmHg, p = 0.04). HPEEP vs MPEEP increased PaO2 (+ 22.00 [+ 1.11; +42.88] mmHg, p = 0.04) or PaO2/FiO2 (+ 42.7 [+ 2.74; +82.67] mmHg, p = 0.04). iPEEP vs MPEEP increased PaO2/FiO2 (+ 115.2 [+ 87.21; +143.20] mmHg, p < 0.001). MPEEP vs LPEP, and HPEEP vs MPEEP increased PaO2 or PaO2/FiO2 significantly with different heterogeneity. HPEEP vs LPEEP increased Cdyn (+ 7.87 [+ 1.49; +14.25] ml/mbar, p = 0.02). MPEEP vs LPEEP, and HPEEP vs MPEEP didn’t impact Cdyn (p = 0.14 and 0.38, respectively). iPEEP vs LPEEP decreased driving pressure (-4.13 [-2.63; -5.63] mbar, p < 0.001). No significant differences in MAP or HR were found between any subgroups.
Conclusion.
HPEEP and iPEEP during PNP in non-obese patients may improve oxygenation, increase Cdyn without clinically significant changes in MAP and HR. MPEEP could be insufficient to increase respiratory compliance and improve oxygenation. LPEEP could lead to hypoxemia and decreased respiratory compliance.
PROSPERO REGISTRATION:
CRD42022362379; registered October 09, 2022
Chromosome and nucleoli diversity in some species of Commelina L. (Commelinaceae) from Ethiopia
Authors: Gebrechristos Samuel , Kiros Gebrehiwet , Mulaw Guesh , Gebreabzgi Bahlibi
Published: May 2023

Background With about 100 species, Commelina is the largest genus of Commelinaceae in Africa. Although medicinal and economic benefits had been studied extensively, little is known about its cytological analysis. Hence, this study will focus on chromosome and nucleoli analysis of selected species of Commelina L. Somatic chromosomes were prepared from root tips that emerged from the nodes of stem cuttings that were made to stand submerged in water. The roots were pretreated in 8-hydroxyquinelin 3–5 hrs followed by fixation in 3:1 ethanol: acetic acid for 1–24 hrs at 4OC. Air-dry slides were prepared following cellulase and pectinase maceration at 37OC, the preparation was stained in Giemsa stain (PH 6.4), rinsed and mounted. Nucleoli were stained in silver nitrate solution.Results Chromosome numbers and Karyotype formula of the four species were found as C. africana 2n = 2x = 30 (12m + 10sm + 8st), C. benghalensis 2n = 6x = 66 (36m + 24sm + 6st), C. diffusa (Ginchi) 2n = 66 (28m + 26sm + 12st), C. diffusa (Jimma) 2n = 2x = 30 (10m + 8sm + 12st) and C. subulata 2n = 2x = 30 (18m + 10sm + 2st). According to Stebbins karyotype asymmetry, the karyotypes of C. africana and C. subulata were 2A type, while that of C. benghalensis and C. diffusa (Ginchi) were 2B type. 3A asymmetry type was obtained for C. diffusa (Jimma). Karotypes of Tradescantia were found to be monomodal for the Commelina species of the studied plant materials. Satellites were observed for species C. africana and C. diffusa with variation in number ranging from 2 to 6. The maximum number of nucleoli observed varies from two to four for Commelina.Conclusions This study reported karyotype and nucleoli of the Ethiopian Commelinaceae for the first time. The current investigation can be considered as an additional karyotype data to the earlier meiosis report for Ethiopian materials.
Integrative Transcriptomic Profiling Reveals Novel Biomarkers in Wilms Tumor
Authors: Avčin Simona Lucija , Črepinšek Klementina , Bizjan Barbara Jenko , Šket Robert , Kovač Jernej , Vrhovšek Blaž , Blazina Jerca , Blatnik Olga , Kordič Robert , Kitanovski Lidija , Jazbec Janez , Debeljak Maruša , Tesovnik Tine
Published: May 2023

Background: This study aimed to identify relevant transcriptomic universal biomarkers for Wilms tumor, the most common pediatric kidney cancer, independent of the histological type and stage.
Methods: Using next-generation sequencing, we analyzed the miRNA profiles of 74 kidney samples, which were divided into two independent groups: fresh frozen tissue and formalin-fixed paraffin-embedded tissue samples. Subsequent mRNA expression profiling and pathway analysis were performed to define the interplay and potential involvement of miRNAs and mRNA in Wilms tumor.
Results: Comparative analysis revealed 41 differentially expressed miRNAs, with 27 miRNAs having decreased expression and 14 miRNAs having increased expression in Wilms tumor tissue compared to healthy kidney tissue. Among global mRNA transcriptomic profile differences, cross-sectional analysis suggested only a limited list of genes potentially regulated by differentially expressed miRNAs in Wilms tumor.
Conclusions: Overall, our study is the first to determine the complete comprehensive miRNA and mRNA profiling of Wilms tumor using a multi-omics next-generation sequencing and bioinformatics approach, providing better insights into Wilms tumor pathogenesis. Identified universal Wilms tumor miRNAs have clear potential as biomarkers for the diagnosis and treatment of Wilms tumor, regardless of histological subtype and disease stage.
Design and Assessment of a Smartphone-based Educational Software for Malaria Detection
Authors: Alizadeh Ismaeil , Yousefi Saideh , Afshar Abbas Aghaei , Gidiglo Godwin Nutifafa , shirani-Bidabadi Leila , Gorouhi Mohammad Amin
Published: May 2023

Background Malaria is among the most important parasitic diseases transmitted by mosquitoes. It is an important health problem in many countries, especially tropical and subtropical countries. The aims of this study were to design an educational software for malaria and evaluate the satisfaction level of the software application among students of Vector Biology and Disease Control in Kerman University of Medical Sciences.Methods This descriptive cross-sectional study was conducted in the following two stages: 1. design and creation of educational software for Malaria and 2. Assessment of the level of satisfaction in the use of an electronic questionnaire among 75 students. Finally, the data were analyzed by SPSS software version 24.Results In the present study, educational software for malaria was designed and created. The results of the study showed that 53 (70.7%) of the participants were females and 22 (29.3%) of them were males. According to the results, 100% of users considered the software installation, size and working speed easy and appropriate. Totally, 93.3% of students stated that images in the software could convey the necessary content. Also, 100% of users found it easy to use the software and to communicate with the software developers. According to the results, more than 80% of students in this study preferred using this educational software to reading texts.Conclusion This educational software is recommended for use by the target community, experts in the healthcare sector and students in medical universities, especially students of Vector Biology and Control.
Direct syntheses of stereoisomers of 3-fluoro GABA and β-fluoroamine analogues of the calcium receptor (CaR) agonists, cinacalcet, tecalcet, fendilines and NPS R-467
Authors: Renault Yohann J. G. , Diao Jiayin , Cordes B B , Leach Katie , O'Hagan David
Published: May 2023

Synthetic routes following a sequential MacMillan organocatalytic asymmetric a-fluorination protocol for aldehydes and then reductive amination, has allowed ready access to bioactive b-fluoroamines. The approach is demonstrated with a short synthesis of (S)-3-fluoro-g-aminobutyric acid (3F-GABA) and was extended to b-fluoroamine stereoisomers of cinacalcet, tecalcet, fendilines and NPS R-467, all allosteric modulators of the calcium receptor (CaR). Stereoisomers of the fluorinated calcimimetic analogues were then assayed in a CaR receptor assay and a comparison of b-fluoroamine matched pair stereoisomers revealed a binding mode preference to the receptor as deduced from conformations which will be favoured as a consequence of the electrostatic gauche effect.