bluuebunny/crossref_metadata_2025 huggingface dataset:
The Well-Being of Elite Volleyball Athletes: A Scoping Review of Methods Using Wellness Questionnaires
Authors: Rebelo André , Pereira João R. , Martinho Diogo V. , Valente-dos-Santos João
Published: August 2023

The purpose of the current review was to systematically and critically evaluate the subjective methods used to evaluate well-being of elite volleyball athletes. According to the results of this scoping review, there is a lack of comprehensive well-being assessment within the volleyball literature as the questionnaires used with these athletes primarily focus on stress, recovery, mood states, and anxiety. While general well-being measures can provide valuable insights, there is a need for measures that consider the unique experiences, challenges, and contexts of athletes. Also, there is an inconsistent definition of well-being in the volleyball literature. This can lead to a piecemeal approach, where different aspects of well-being are assessed in isolation, without a clear understanding of how they fit together. Therefore, to gain a more comprehensive understanding of volleyball athletes’ well-being, it is important to incorporate measures that assess subjective, psychological, and social well-being. In conclusion, while current assessment tools provide important insights into volleyball athletes’ well-being, there is a clear need for more holistic and sport-specific measures. By expanding an understanding and assessment of well-being, there can be better support for athletes’ overall health, satisfaction, and performance.
Southern Alaska as a source of atmospheric mineral dust and ice-nucleating particles
Authors: Barr Sarah L. , Wyld Bethany , McQuaid James B. , Neely III Ryan R. , Murray Benjamin J.
Published: August 2023

Ice-nucleating particles (INPs) influence cloud radiative properties and climate; however, INP sources and concentrations are poorly constrained, particularly in high-latitude regions. Southern Alaska is a known source of high-latitude dust, but its contribution to atmospheric mineral dust and INP concentrations has not been quantified. We show that glacial dust collected in southern Alaska is an effective ice-nucleating material under conditions relevant for mixed-phase clouds and is more active than low-latitude dust because of a biological component that enhances its activity. We use dispersion modeling to show that this source contributes to the regional INP population and that the dust emitted is transported over a broad area of North America, reaching altitudes where it could cause cloud glaciation. Our results highlight the importance of quantifying emissions and ice-nucleating characteristics of high-latitude dusts and suggest that the ice-nucleating ability of emitted dust in these regions should be represented in models using different parametrizations to low-latitude dust.
Supplemental Material: Formation and evolution of a magmatic system in the Indian passive margin: Insights from the Triassic Yumai Complex from the eastern Tethyan Himalaya
Authors: Wang Chao , al. et
Published: August 2023

Analytical methods and data: Text S1, Figure S1, and Tables S1 and S2.   
Associations between cardiorespiratory fitness in youth and the incidence of site-specific cancer in men: a cohort study with register linkage
Authors: Onerup Aron , Mehlig Kirsten , Geijerstam Agnes af , Ekblom-Bak Elin , Kuhn Hans Georg , Lissner Lauren , Åberg Maria , Börjesson Mats
Published: August 2023

Objectives

To assess the associations between cardiorespiratory fitness (CRF) in young men and the incidence of site-specific cancer.

Methods

A Swedish population-based cohort study with register linkage of men who underwent military conscription in 1968–2005 was undertaken. CRF was assessed by maximal aerobic workload cycle test at conscription. Cox regression models assessed linear associations and included CRF, age, year and site of conscription, body mass index and parental level of education. CRF was also categorised into low, moderate and high for facilitated interpretation and results comparing high and low CRF are reported.

Results

Primary analyses were performed in 1 078 000 men, of whom 84 117 subsequently developed cancer in at least one site during a mean follow-up of 33 years. Higher CRF was linearly associated with a lower hazard ratio (HR) of developing cancer in the head and neck (n=2738, HR 0.81, 95% CI 0.74 to 0.90), oesophagus (n=689, HR 0.61, 95% CI 0.50 to 0.74), stomach (n=902, HR 0.79, 95% CI 0.67 to 0.94), pancreas (n=1280, HR 0.88, 95% CI 0.76 to 1.01), liver (n=1111, HR 0.60, 95% CI 0.51 to 0.71), colon (n=3222, HR 0.82, 95% CI 0.75 to 0.90), rectum (n=2337, HR 0.95, 95% CI 0.85 to 1.05), kidney (n=1753, HR 0.80, 95% CI 0.70 to 0.90) and lung (n=1635, HR 0.58, 95% CI 0.51 to 0.66). However, higher CRF predicted a higher hazard of being diagnosed with prostate cancer (n=14 232, HR 1.07, 95% CI 1.03 to 1.12) and malignant skin cancer (n=23 064, HR 1.31, 95% CI 1.27 to 1.36).

Conclusion

We report a number of protective associations between higher CRF in healthy young men and the subsequent hazard of site-specific cancers. These results have implications for public health policymaking, strengthening the incentive to promote health through improving CRF in youth.
Multiple molecular diagnoses in the field of intellectual disability and congenital anomalies: 3.5% of all positive cases
Authors: Racine Caroline , Denommé-Pichon Anne-Sophie , Engel Camille , Tran Mau-them Frederic , Bruel Ange-Line , Vitobello Antonio , Safraou Hana , Sorlin Arthur , Nambot Sophie , Delanne Julian , Garde Aurore , Colin Estelle , Moutton Sébastien , Thevenon Julien , Jean-Marçais Nolwenn , Willems Marjolaine , Geneviève David , Pinson Lucile , Perrin Laurence , Laffargue Fanny , Lespinasse James , Lacaze Elodie , Molin Arnaud , Gerard Marion , Lambert Laetitia , Benigni Charlotte , Patat Olivier ,   Orphanomix Physician's Group, Bourgeois Valentin , Poe Charlotte , Chevarin Martin , Couturier Victor , Garret Philippine , Philippe Christophe , Duffourd Yannis , Faivre Laurence , Thauvin-Robinet Christel
Published: August 2023

Purpose

Wide access to clinical exome/genome sequencing (ES/GS) enables the identification of multiple molecular diagnoses (MMDs), being a long-standing but underestimated concept, defined by two or more causal loci implicated in the phenotype of an individual with a rare disease. Only few series report MMDs rates (1.8% to 7.1%). This study highlights the increasing role of MMDs in a large cohort of individuals addressed for congenital anomalies/intellectual disability (CA/ID).

Methods

From 2014 to 2021, our diagnostic laboratory rendered 880/2658 positive ES diagnoses for CA/ID aetiology. Exhaustive search on MMDs from ES data was performed prospectively (January 2019 to December 2021) and retrospectively (March 2014 to December 2018).

Results

MMDs were identified in 31/880 individuals (3.5%), responsible for distinct (9/31) or overlapping (22/31) phenotypes, and potential MMDs in 39/880 additional individuals (4.4%).

Conclusion

MMDs are frequent in CA/ID and remain a strong challenge. Reanalysis of positive ES data appears essential when phenotypes are partially explained by the initial diagnosis or atypically enriched overtime. Up-to-date clinical data, clinical expertise from the referring physician, strong interactions between clinicians and biologists, and increasing gene discoveries and improved ES bioinformatics tools appear all the more fundamental to enhance chances of identifying MMDs. It is essential to provide appropriate patient care and genetic counselling.
Groundwater Quality and Public Health
Authors: Xie Xianjun , Shi Jianbo , Pi Kunfu , Deng Yamin , Yan Bing , Tong Lei , Yao Linlin , Dong Yiran , Li Junxia , Ma Liyuan , Zheng Chunmiao , Jiang Guibin
Published: August 2023

Groundwater deterioration due to enrichment with contaminants of either geogenic or anthropogenic origin has adversely affected safe water supply for drinking and irrigation, with pervasive impacts on human health and ecosystem functions. However, the spatiotemporal evolution and public health effects of groundwater quality remain unclarified, posing a grand challenge for the safe and sustainable supply of global groundwater resources. This article provides a state-of-the-art review of the complexity and dynamics of groundwater quality, as well as the impacts of various groundwater substances on human health. In particular, knowledge is growing about the health impacts of key substances ranging from nutritional elements (e.g., Ca2+, Mg2+) to pollutants (e.g., heavy metals/metalloids, persistent organic pollutants, and emerging contaminants) and, further, to pathogenic microorganisms to which the human body can be exposed through multiple patterns of groundwater use. Proliferating concerns at the same time call for enhancing science-based governance directives, economic policies, and management strategies coordinating groundwater quality. We propose that safeguarding groundwater-dependent public health needs concerted efforts in source control, cross-scale rehabilitation, and social hydrology-based groundwater governance.
Fixing Our Public Discounting Systems
Authors: Cherbonnier Frédéric , Gollier Christian
Published: August 2023

Most Western countries use a single discount rate to evaluate public investments and policies. This ignores the differential cost of risk, in a world where most risk markets exhibit surprisingly large prices of risk. The current discounting guidelines generate a misallocation of capital that entails a large welfare cost. We claim that the well-established asset pricing literature provides a strong normative justification in favor of risk-adjusting discount rates. More specifically, project-specific discount rates should be increasing in the income elasticity of the project's net benefit. This will favor projects whose net benefit materializes preferentially in low-income states, thereby recognizing their insurance benefit ex ante. The intuition is simple, the welfare benefit of the reform is large, and the methodology only requires evaluators to estimate an income elasticity on top of what is required in the current approach. It is time to fix our public discounting systems.
Cultural Psychology: Beyond East and West
Authors: Kitayama Shinobu , Salvador Cristina E.
Published: August 2023

Research in cultural psychology over the last three decades has revealed the profound influence of culture on cognitive, emotional, and motivational processes shaping individuals into active agents. This article aims to show cultural psychology's promise in three key steps. First, we review four notable cultural dimensions believed to underlie cultural variations: independent versus interdependent self, individualism versus collectivism, tightness versus looseness of social norms, and relational mobility. Second, we examine how ecology and geography shape human activities and give rise to organized systems of cultural practices and meanings, called eco-cultural complexes. In turn, the eco-cultural complex of each zone is instrumental in shaping a wide range of psychological processes, revealing a psychological diversity that extends beyond the scope of the current East–West literature. Finally, we examine some of the non-Western cultural zones present today, including Arab, East Asian, Latin American, and South Asian zones, and discuss how they may have contributed, to varying degrees, to the formation of the contemporary Western cultural zone.
PINN‐CDR: A Neural Network‐Based Simulation Tool for Convection‐Diffusion‐Reaction Systems
Authors: Hou Qingzhi , Du Honghan , Sun Zewei , Wang Jianping , Wang Xiaojing , Wei Jianguo
Published: August 2023

In this paper, a discretization‐free approach based on the physics‐informed neural network (PINN) is proposed for solving the forward and inverse problems governed by the nonlinear convection‐diffusion‐reaction (CDR) systems. By embedding physical information described by the CDR system in the feedforward neural networks, PINN is trained to approximate the solution of the system without the need of labeled data. The good performance of PINN in solving the forward problem of the nonlinear CDR systems is verified by studying the problems of gas‐solid adsorption and autocatalytic reacting flow. For CDR systems with different Péclet number, PINN can largely eliminate the numerical diffusion and unphysical oscillations in traditional numerical methods caused by high Péclet number. Meanwhile, the PINN framework is implemented to solve the inverse problem of nonlinear CDR systems and the results show that the unknown parameters can be effectively recognized even with high noisy data. It is concluded that the established PINN algorithm has good accuracy, convergence, and robustness for both the forward and inverse problems of CDR systems.
Reconciling Airborne Disease Transmission Concerns with Energy Saving Requirements: The Potential of UV-C Pathogen Deactivation and Air Distribution Optimization
Authors: Gaillard Antoine , Lohse Detlef , Bonn Daniel , Yigit Fahmi
Published: August 2023

The COVID-19 pandemic caused a paradigm shift in our way of using heating, ventilation, and air-conditioning (HVAC) systems in buildings. In the early stages of the pandemic, it was indeed advised to reduce the reuse and thus the recirculation of indoor air to minimize the risk of contamination through inhalation of virus-laden aerosol particles emitted by humans when coughing, sneezing, speaking, or breathing. However, such recommendations are not compatible with energy saving requirements stemming from climate change and energy price increase concerns, especially in winter and summer when the fraction of outdoor air supplied to the building needs to be significantly heated or cooled down. In this experimental study, we aim at providing low-cost and low-energy solutions to modify the ventilation strategies currently used in many buildings to reduce the risk of respiratory disease transmission. Measurements of the indoor air bacterial concentration in a typical office building reveal that ultraviolet germicidal irradiation (UVGI) modules added to the HVAC system are very efficient at inactivating pathogens present in aerosols, leading to indoor concentrations as low as outdoor concentrations, even with significant indoor air recirculation. Moreover, measurements of the CO2 and aerosol air concentration reveal that, with air supply vents placed in the ceiling, placing the air exhaust vents near the floor instead of on the ceiling can improve the ventilation capacity in terms of effective flow rate, with significant consequences in terms of energy savings.
Characterization of Bismuth Composited to Carbon Nanotube-Coated Titanium Cathode in Electro-Fenton System
Authors: Wang Yi-Ta , Hsieh Yi-Chi , Lin Yue-Sheng
Published: August 2023

Bismuth (Bi) is a highly reactive catalyst for the generation of hydroxyl (∙OH) radicals. Cathodes constructed from composites of Bi and carbon nanotube (CNT) exhibit high stability and low resistance, which enhance their electron transfer capability. In this work, a titanium substrate was coated with multi-walled carbon nanotube (MWCNT/Ti) using electrophoretic deposition process, followed by electrodeposition of Bi onto the MWCNT-coated Ti (Bi/MWCNT/Ti). The effects of Bi electrodeposition time on the surface morphology of Bi/MWCNT/Ti cathodes were investigated by scanning electron microscopy and energy-dispersive X-ray spectroscopy, and the electrochemical characteristics of each cathode were identified via a series of electrochemical analyses further. The results demonstrated that electrodeposition at −0.85 V vs. Ag/AgCl for 5 min revealed uniform distribution of dense Bi across the surface of cathode, which provides better hydrophilicity for cathode and promotes highest electron transfer rates, respectively; when the Bi/MWCNT/Ti cathode was used as an electro-Fenton (EF) cathode, the EF system achieved a rhodamine B degradation rate of 80.8% after 30 min, which is a significant increase (83.63%) than the unmodified Ti cathode. The use of Bi in EF cathodes improves the efficiency of the EF process.
Figure S2 from A Genomic Analysis of Esophageal Squamous Cell Carcinoma in Eastern Africa
Authors: Van Loon Katherine , Mmbaga Elia J. , Mushi Beatrice P. , Selekwa Msiba , Mwanga Ally , Akoko Larry O. , Mwaiselage Julius , Mosha Innocent , Ng Dianna L. , Wu Wei , Silverstein Jordyn , Mulima Gift , Kaimila Bongani , Gopal Satish , Snell Jeff M. , Benz Stephen Charles , Vaske Charles , Sanborn Zack , Sedgewick Andrew J. , Radenbaugh Amie , Newton Yulia , Collisson Eric A.
Published: August 2023

Supplementary Figure S2. Expression of Mutsig CV genes in cohorts from Tanzania (Panel A) and Malawi (Panel B).
Supplementary Table 3 from Changes in Geographic Variation and Socioeconomic Inequalities in Esophageal Cancer Mortality in China, 1973–2017
Authors: Ran Xianhui , Zheng Rongshou , Zeng Hongmei , Zhang Siwei , Sun Kexin , Han Bingfeng , Wang Shaoming , Chen Ru , Li Li , Wei Wenqiang , He Jie
Published: August 2023

Supplementary Table 3 shows the difference in age-standardized esophageal cancer mortality rates between 90th and 10th percentile counties by province and time period.
Supplementary Table S2 from Prediagnostic Prescription Antibiotics Use and Survival in Patients with Colorectal Cancer: A Swedish National Register-based Study
Authors: Lu Sai San Moon , Rutegård Martin , Ahmed Maghfoor , Häggström Christel , Gylfe Åsa , Harlid Sophia , Van Guelpen Bethany
Published: August 2023

Supplementary Table S2: Reported number of defined daily doses (DDD) for specific antibiotics
Supplementary Table S1 from Prediagnostic Prescription Antibiotics Use and Survival in Patients with Colorectal Cancer: A Swedish National Register-based Study
Authors: Lu Sai San Moon , Rutegård Martin , Ahmed Maghfoor , Häggström Christel , Gylfe Åsa , Harlid Sophia , Van Guelpen Bethany
Published: August 2023

Supplementary Table S1: Classification of antibiotics types and indications
Supplementary Figure 2 from Changes in Geographic Variation and Socioeconomic Inequalities in Esophageal Cancer Mortality in China, 1973–2017
Authors: Ran Xianhui , Zheng Rongshou , Zeng Hongmei , Zhang Siwei , Sun Kexin , Han Bingfeng , Wang Shaoming , Chen Ru , Li Li , Wei Wenqiang , He Jie
Published: August 2023

Supplementary Figure 2 was a scatter diagram of the age-standardized esophageal cancer mortality rate in 1973-1975 and the absolute change in mortality rates from 1973–1975 to 2015–2017.
Supplementary Data File S3 from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

Page 1: RT-qPCR primers used for DUB array; Page 2: Primers used for ChIP-qPCR analysis; Page 3: shRNA sequences used for EWS::FLI1 and USP1 knockdown cell line generation; Page 4: gRNA sequences used for USP1 knockout cell line generation; Page 5: List of working dilutions for antibodies used in this study; Page 6: List of reagents, with product information and RRIDs, used in this study; Page 7: List of biological resources, with product information and RRIDs, used in this study
Supplementary Data File S1 from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

RT-qPCR array of DUBs. RQ values of EWS cell lines normalized to GAPDH, represented as fold change relative to hMSC.
Figure S5 from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

Figure S5 shows EWS cells are sensitive to doxorubicin and etoposide treatment.
Figure S3 from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

Figure S3 shows USP1 knockdown or inhibition induces apoptotic cell death.
Figure S2 from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

Figure S2 shows USP1 knockdown or inhibition decreases cell growth.
Data from USP1 Expression Driven by EWS::FLI1 Transcription Factor Stabilizes Survivin and Mitigates Replication Stress in Ewing Sarcoma
Authors: Mallard Halle J. , Wan Shibiao , Nidhi Prakriti , Hanscom-Trofy Yvan D. , Mohapatra Bhopal , Woods Nicholas T. , Lopez-Guerrero Jose Antonio , Llombart-Bosch Antonio , Machado Isidro , Scotlandi Katia , Kreiling Natasha F. , Perry Megan C. , Mirza Sameer , Coulter Donald W. , Band Vimla , Band Hamid , Ghosal Gargi
Published: August 2023

Abstract
In this study, we identify USP1 as a transcriptional target of EWS::FLI1 and demonstrate the requisite function of USP1 in Ewing sarcoma (EWS) cell survival in response to endogenous replication stress. EWS::FLI1 oncogenic transcription factor drives most EWS, a pediatric bone cancer. EWS cells display elevated levels of R-loops and replication stress. The mechanism by which EWS cells override activation of apoptosis or cellular senescence in response to increased replication stress is not known. We show that USP1 is overexpressed in EWS and EWS::FLI1 regulates USP1 transcript levels. USP1 knockdown or inhibition arrests EWS cell growth and induces cell death by apoptosis. Mechanistically, USP1 regulates Survivin (BIRC5/API4) protein stability and the activation of caspase-9 and caspase-3/7 in response to endogenous replication stress. Notably, USP1 inhibition sensitizes cells to doxorubicin and etoposide treatment. Together, our study demonstrates that USP1 is regulated by EWS::FLI1, the USP1–Survivin axis promotes EWS cell survival, and USP1 inhibition sensitizes cells to standard of care chemotherapy.
Implications:
High USP1 and replication stress levels driven by EWS::FLI1 transcription factor in EWS are vulnerabilities that can be exploited to improve existing treatment avenues and overcome drug resistance.
Supplementary Figure 2 from PARP inhibitors effectively reduce MAPK inhibitor resistant melanoma cell growth and synergize with MAPK inhibitors through a synthetic lethal interaction in vitro and in vivo
Authors: Fröhlich Lisa Marie , Niessner Heike , Sauer Birgit , Kämereit Sofie , Chatziioannou Eftychia , Riel Simon , Sinnberg Tobias , Schittek Birgit
Published: August 2023

Synergistic interaction of PARPi and MAPKi treatment
Supplementary Table 5 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Pearson correlation (r) analysis of ICOS versus PD-L1 (CD274) in different tumor type.
Supplementary Table 2 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Table S2
Supplementary Movie 1 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Supplementary Movie 1
Supplementary Figure 8 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Pharmacokinetic analysis of the mIgG1 anti-ICOS antibody (7E.17G9) in the peripheral blood of EMT6 tumor-bearing mice.
Supplementary Figure 7 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Healthy human donor CD4+ T cells were pre-activated with antiCD3/CD28 for 48 hours and then re-stimulated with plate-bound anti-CD3 in the presence of increasing concentrations of soluble or plate-bound feladilimab or isotype control for 72 hours. IFNg levels were then assessed from cell-free supernatants using Meso Scale Discovery (MSD)-based detection.
Supplementary Figure 3 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Affinity and kinetics
Supplementary Figure 12 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

Clinical case studies of feladilimab monotherapy and in combination with pembrolizumab.
FIGURE 4 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

ICOS agonist mAbs demonstrate improved antitumor activity in combination with PD-1 blockade. EMT6 (subcutaneous) tumor-bearing BALB/c mice were administered intraperitoneally biweekly with anti-mouse ICOS mAb [7E.17G9 (mIgG1)], anti-PD-1 mAb (RMP1-14), or isotype controls (mIgG1 and rat IgG2a, respectively) alone and in combination for a total of six doses. Mice were evaluated for pharmacodynamic changes (B and C) within tumors, tumor growth (D), and survival (E). As illustrated in A, transcriptional analysis was performed (n = 5–7) on tumor tissue harvested from mice 48 hours after second (B) and third doses (C) of indicated mAbs; raw data in Supplementary Table S4. Each line in D represents an individual mouse (n = 10/group). Tumor-free mice at study termination are indicated within each subpanel. E, Kaplan–Meier plot illustrating OS in D. F,ICOS expression following ex vivo anti–PD-1 (pembrolizumab) or vehicle control treatment of tumor slices from patients with HNSCC for 48 hours. Each symbol represents an individual human tumor sample (n = 50/group). G, Fold change in IFNγ production by TILs from dissociated NSCLC tumor samples (n = 5–6 samples/group) following exposure to anti-CD3 (plate-bound, 0.6 μg/mL) in concert with anti-PD-1 (pembrolizumab, soluble) or feladilimab (plate-bound) alone or in combination for 24 hours. H, A2058 (subcutaneous) tumor-bearing NSG mice were administered intraperitoneally biweekly with feladilimab and anti-PD-1 (pembrolizumab) alone or in combination for a total of six doses and assessed for tumor growth inhibition (n = 10/group). Data in F–H are represented as mean ± s.e.m. Significance in G determined by unpaired Student t test. Despite trends in tumor growth kinetics following combination treatment, the curves in H were not significantly different as determined by one-way ANOVA. ANOVA, analysis of variance; HNSCC, head and neck squamous cell carcinoma; ICOS, inducible T cell costimulator; IFN, interferon; i.p., intraperitoneal injection; mAb, monoclonal antibody; NSCLC, non–small cell lung carcinoma; OS, overall survival; PD-1, programmed cell death protein 1; s.e.m., standard error of the mean; TIL, tumor-infiltrating lymphocyte.
FIGURE 3 from Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade
Authors: Yadavilli Sapna , Waight Jeremy D. , Brett Sara , Bi Meixia , Zhang Tianqian , Liu Yao-Bin , Ellis Catherine , Turner David C. , Hahn Ashleigh , Shi Hong , Seestaller-Wehr Laura , Jing Junping , Xie Qing , Shaik Jafar Sadik , Ji Xiao , Gagnon Robert , Fieles William , Hook Laura , Grant Steven , Hopley Stephanie , DeYoung M. Phillip , Blackwell Christina , Chisamore Michael , Biddlecombe Robert , Figueroa David J. , Hopson Christopher B. , Srinivasan Roopa , Smothers James , Maio Michele , Rischin Danny , Olive Daniel , Paul Elaine , Mayes Patrick A. , Hoos Axel , Ballas Marc
Published: August 2023

ICOS agonist mAbs demonstrate single-agent antitumor activity. A–C, EMT6 (subcutaneous) tumor-bearing BALB/c mice were administered intraperitoneally biweekly with anti-mouse ICOS mAb [7E.17G9 (mIgG1)] or isotype control (mIgG1) for a total of six doses and evaluated for tumor growth (A), survival (B), and pharmacodynamic changes (C) within tumors. Each line in A represents an individual mouse (n = 10/group). Number of tumor-free mice at study termination are indicated within each subpanel. B, Kaplan–Meier plot illustrating OS in A. C, Percentage of tumor-infiltrating CD8+ T cells, Treg cells, and associated CD8:Treg cell ratio 48 hours after the third dose of anti-ICOS mAb (10 μg, ∼study day 7) assessed using flow cytometry (nonspecific binding was blocked with human or mouse Fc block). Each symbol represents an individual mouse. D–F, A549 (subcutaneous) tumor-bearing NSG mice were administered intraperitoneally biweekly with feladilimab or isotype control for a total of six doses. D, A549 tumor growth kinetics for controls (PBS or isotype) and feladilimab-treated groups (0.04 mg/kg, black; 0.4 mg/kg, red). Percentage of tumor-infiltrating ICOS+ CD8+ T cells (E; assessed using flow cytometry with nonspecific binding blocked using human or mouse Fc block) and associated CD8:Treg cell ratio (F) 48 hours after the fourth dose of feladilimab (∼study day 21). Data in C, D, E, and F represent the mean ± s.e.m. Significance was determined by unpaired Student t test. See Supplementary Fig. S1 for flow cytometry gating strategy for C, E, and F. CD8/Treg ratios were calculated on the basis of percent (%) positive CD8s (of live CD45+) and FoxP3/CD25+ Treg cells (of live CD45+ CD4+ cells). ICOS, inducible T-cell costimulator; i.p., intraperitoneal injection; mAb, monoclonal antibody; OS, overall survival; s.e.m., standard error of the mean.
Acute Abdominal Pain as the Initial Presentation of an Acquired C1 Inhibitor Deficiency
Authors: Pinto Ana Raquel , Carolino Fabrícia
Published: August 2023

Introduction: Acquired angioedema (AAE), a rare cause of adult-onset non-urticarial mucocutaneous angioedema, can present as acute abdomen, a frequent complaint in the emergency room (ER), often leading to unnecessary and potentially harmful procedures. Case Presentation: We report a 47-year-old hypertense male, controlled with an angiotensin converting enzyme inhibitor (ACEI), who presented in the ER with progressively worsening abdominal pain, nausea, and vomiting, and a radiologic workup revealing small intestine thickening, initially diagnosed with ACEI-induced angioedema. However, further investigation revealed low serum levels of C4, C1q, and C1 inhibitors, with an abnormal function of the latter, favoring the diagnosis of AAE instead. The frequent association of this condition with lymphoproliferative disorders encouraged further studies, which unveiled a monoclonal gammopathy IgM/Kappa, representing an increased risk of Waldenström macroglobulinemia, non-Hodgkin lymphoma, and multiple myeloma. Discussion: AAE should be regarded as an important differential diagnosis in patients presenting with acute abdomen in the ER, especially when more common causes are excluded. A correct and early diagnosis may represent a chance for a better prognosis of underlying diseases.
Centenário do falecimento de Oscar Freire
Authors: Lima Lamartine de Andrade
Published: August 2023

O presente texto conta a história de Oscar Freire, eminente médico, professor e pesquisador baiano que teve papel fundamental no desenvolvimento da Medicina Legal no Brasil.
Design Choices for Crowdsourcing Implicit Discourse Relations: Revealing the Biases Introduced by Task Design
Authors: Pyatkin Valentina , Yung Frances , Scholman Merel C. J. , Tsarfaty Reut , Dagan Ido , Demberg Vera
Published: August 2023

Disagreement in natural language annotation has mostly been studied from a perspective of biases introduced by the annotators and the annotation frameworks. Here, we propose to analyze another source of bias—task design bias, which has a particularly strong impact on crowdsourced linguistic annotations where natural language is used to elicit the interpretation of lay annotators. For this purpose we look at implicit discourse relation annotation, a task that has repeatedly been shown to be difficult due to the relations’ ambiguity. We compare the annotations of 1,200 discourse relations obtained using two distinct annotation tasks and quantify the biases of both methods across four different domains. Both methods are natural language annotation tasks designed for crowdsourcing. We show that the task design can push annotators towards certain relations and that some discourse relation senses can be better elicited with one or the other annotation approach. We also conclude that this type of bias should be taken into account when training and testing models.
Reconsidering the Turkish–Islamic Synthesis
Authors: Elhan Nail , Şirin Başar
Published: August 2023

Turkish–Islamic Synthesis has been an influential doctrine since the 1970s in Turkey. It emerged as a national/cultural reaction against the rising influence of the leftist and radical Islamist movements and has been discussed within these contexts. This study will expand the scope of the concept and offer Iran/Shi‘ism as a threat to Turkey’s national/religious integrity within the context of Turkish–Islamic Synthesis. The historical/ideological rivalry between the Sunni/Shi‘a and Turks/Iranians reached its peak after the Iranian Revolution in 1979. This study, which examines the issue with regards to the otherness and friend/enemy distinction perspectives, aims to include Iran and Shi‘ism in the discourse on Turkish–Islamic Synthesis and will use primary sources on the subject.
Laṭxāna (1950–1954), an Intellectual Commune in Quetta (Baluchistan)
Authors: Columeau Julien
Published: August 2023

This paper retraces the history, activities, and contribution of an intellectual commune active in Quetta, Pakistan between 1950 and 1954: the ‘Laṭxāna commune’. Laṭxāna (Psht. ‘House of idleness’) is the name of a place in which Baluch, Pashtun, Urdu-speaking and Sindhi intellectuals settled in 1950. Laṭxāna’s intellectuals were in close contact with the Communist Party of Pakistan and its cultural branch, the Progressive Writers’ Association, and attempted to spread socialist or communist thought in Baluchistan. Following an agenda outlined by communist and progressive writers, they set out to develop literature in the languages of Baluchistan, launching a Baluchi literary association and a Pashto-language journal and publishing the first collections of modern Baluchi poetry. Laṭxāna’s members also promoted their outlook through journalism, and edited journals, such as Xāwar, Nawā-e waṭan and Ciltan. In 1954, the Laṭxāna intellectuals—who had so far been simple representatives of the Communists or Progressives in Baluchistan—started their own political movement. They created a political party and published a manifesto, which called for a socialist Baluchistan free from the influence of landowners and feudal leaders. Alongside ideological disagreements, the arrest of some of the commune’s prominent members finally led to the closure of Laṭxāna, but the group nevertheless had a long-lasting influence on Baluchistan’s political and intellectual landscape. In this paper, I shall discuss the commune’s literary, journalistic, and political contributions, notably through the accounts of its founding fathers, Mir Abdullah Jamaldini and Sain Kamal Khan Sherani.
Johann P. Arnason’s Analysis of Communism and the Debate on Soviet Modernity
Authors: Maslovskiy Mikhail
Published: August 2023

Johann P. Arnason has offered a comprehensive sociological analysis of the origins, historical dynamics and decline of the alternative communist model of modernity. At the same time, in the field of Soviet studies two distinct approaches have been formed which stress the modern character of Soviet society or its neo-traditionalist aspects. Arnason’s work can contribute to bridging the gap between these approaches. His ideas are also relevant for explaining the collapse of the Soviet system and assessing the role of historical legacies in post-Soviet states. Combining Arnason’s theoretical perspective and recent approaches in history and comparative politics may result in a deeper understanding of the Soviet model as a specific type of modern society and the consequences of its disintegration.
Mind your app: Could plant ID applications lead to an increase in extinction risk?
Authors: BERJANO REGINA , LÓPEZ-TIRADO JAVIER , MARTÍN-ESCOBAR IRENE , MARTÍNEZ-SAGARRA GLORIA , NIETO-LUGILDE DIEGO , SÁNCHEZ-ROMERO JIMENA , ESTRELLA MANUEL DE LA
Published: August 2023

N/A
Rare disease variant curation from literature: assessing gaps with creatine transport deficiency in focus
Authors: Lyons Erica L. , Watson Daniel , Alodadi Mohammad S. , Haugabook Sharie J. , Tawa Gregory J. , Hannah-Shmouni Fady , Porter Forbes D. , Collins Jack R. , Ottinger Elizabeth A. , Mudunuri Uma S.
Published: August 2023

Background

Approximately 4–8% of the world suffers from a rare disease. Rare diseases are often difficult to diagnose, and many do not have approved therapies. Genetic sequencing has the potential to shorten the current diagnostic process, increase mechanistic understanding, and facilitate research on therapeutic approaches but is limited by the difficulty of novel variant pathogenicity interpretation and the communication of known causative variants. It is unknown how many published rare disease variants are currently accessible in the public domain.

Results

This study investigated the translation of knowledge of variants reported in published manuscripts to publicly accessible variant databases. Variants, symptoms, biochemical assay results, and protein function from literature on the SLC6A8 gene associated with X-linked Creatine Transporter Deficiency (CTD) were curated and reported as a highly annotated dataset of variants with clinical context and functional details. Variants were harmonized, their availability in existing variant databases was analyzed and pathogenicity assignments were compared with impact algorithm predictions. 24% of the pathogenic variants found in PubMed articles were not captured in any database used in this analysis while only 65% of the published variants received an accurate pathogenicity prediction from at least one impact prediction algorithm.

Conclusions

Despite being published in the literature, pathogenicity data on patient variants may remain inaccessible for genetic diagnosis, therapeutic target identification, mechanistic understanding, or hypothesis generation. Clinical and functional details presented in the literature are important to make pathogenicity assessments. Impact predictions remain imperfect but are improving, especially for single nucleotide exonic variants, however such predictions are less accurate or unavailable for intronic and multi-nucleotide variants. Developing text mining workflows that use natural language processing for identifying diseases, genes and variants, along with impact prediction algorithms and integrating with details on clinical phenotypes and functional assessments might be a promising approach to scale literature mining of variants and assigning correct pathogenicity. The curated variants list created by this effort includes context details to improve any such efforts on variant curation for rare diseases.
RETRACTED ARTICLE: Lactate dehydrogenase D
serves as a novel biomarker for prognosis and immune infiltration in lung
adenocarcinoma
Authors: Zhang Yu , Zhang Tianyi , Zhao Yingdong , Wu Hongdi , Zhen Qiang , Zhu Suwei , Hou Shaoshuai
Published: August 2023

Background

Lung cancer is reported to be the leading cause of death in males
and females, globally. Increasing evidence highlights the paramount importance
of Lactate dehydrogenase D (LDHD) in different types of cancers, though it’s
role in lung adenocarcinoma (LUAD) is still inadequately explored. In this
study, we aimed to investigate and determine the relationship between LDHD and
LUAD.

Methods

The collection of the samples was guided by The Cancer Genome Atlas
(TCGA) datasets and Gene Expression Omnibus (GEO). To ascertain various aspects
around LDHD function, we analyzed different expression genes (DEGs), functional
enrichment, and protein–protein interaction (PPI) networks. The predictive
values for LDHD were collectively determined using the Kaplan–Meier method, Cox
regression analysis, and a nomogram. Evaluation of the immune infiltration
analysis was completed using Estimate and ssGSEA. The prediction of the
immunotherapy response was based on TIDE and IPS. The LDHD expression levels in
LUAD were validated through Western blot, qPCR, and immunohistochemistry
methods. Wound healing and transwell assays were also performed to illustrate
the aggressive features in LUAD cell lines.

Results

The results showed that LDHD was generally downregulated in LUAD
patients, with the low LDHD group presenting a decline in OS, DSS, and PFI.
Enriched pathways, which include pyruvate metabolism, central carbon metabolism,
and oxidative phosphorylation were observed through KEGG analysis. It was also
noted that the expression of LDHD expression was inversely related to immune
cell infiltration and typical checkpoints. The high LDHD group’s response to
immunotherapy was remarkable, particularly in CTAL4 + /PD1- therapy. In vitro
studies revealed that the overexpression of LDHD caused tumor migration and
invasion to be suppressed.

Conclusion

In conclusion, our study revealed that LDHD might be an effective
predictor of prognosis and immune filtration, possibly leading to better choices
for immunotherapy.