bluuebunny/crossref_metadata_2025 huggingface dataset:
Flexible and Integrative Psychiatric Care Based on a Global Treatment Budget: Comparing the Implementation in Germany and Poland
Authors: Schwarz Julian , Cechnicki Andrzej , Godyń Jan , Galbusera Laura , Biechowska Daria , Galińska-Skok Beata , Ciunczyk Izabela , Ignatyev Yuriy , Muehlensiepen Felix , Soltmann Bettina , Timm Jürgen , Peter Sebastian von , Balicki Marek , Wciórka Jacek , Heinze Martin
Published: January 2022

Background: The past decade has witnessed the establishment of flexible and integrative treatment (FIT) models in 55 German and Polish psychiatric catchment areas. FIT is based on a global treatment budget (GTB), which integrates funding of all acute psychiatric hospital services for a regional population. Prior research has identified 11 specific program components of FIT in Germany. In this paper we aim at assessing the applicability of these components to the Polish context and at comparatively analysing FIT implementation in Poland and Germany.

Methods: Qualitative interviews about the applicability of the 11 FIT-specific components were conducted with the program managers of the Polish FIT models (n = 19). Semi-quantitative data on the FIT-specific components were then collected in 19 Polish and 10 German FIT models. We assessed the grading of each component, their overall degree of implementation and compared them between the two countries. In all study hospitals, structural and statistical parameters of service delivery were collected and compared.

Results: The qualitative results showed that the German FIT-specific components are in principle applicable to the polish context. This allowed the comparative assessment of components grading and degree of implementation, which showed only subtle discrepancies between German and Polish FIT models. The little discrepancies point to specific aspects of care such as home treatment, peer support, and cooperation with non-clinical and social welfare institutions that should be further integrated in the components' definition.

Conclusions: The specific program components of FIT as first defined from the German experience, serves as a powerful tool to measure, and evaluate implementation of integrated psychiatric care both within and between health systems.
Lower Levels of Directed Exploration and Reflective Thinking Are Associated With Greater Anxiety and Depression
Authors: Smith Ryan , Taylor Samuel , Wilson Robert C. , Chuning Anne E. , Persich Michelle R. , Wang Siyu , Killgore William D. S.
Published: January 2022

Anxiety and depression are often associated with strong beliefs that entering specific situations will lead to aversive outcomes – even when these situations are objectively safe and avoiding them reduces well-being. A possible mechanism underlying this maladaptive avoidance behavior is a failure to reflect on: (1) appropriate levels of uncertainty about the situation, and (2) how this uncertainty could be reduced by seeking further information (i.e., exploration). To test this hypothesis, we asked a community sample of 416 individuals to complete measures of reflective cognition, exploration, and symptoms of anxiety and depression. Consistent with our hypotheses, we found significant associations between each of these measures in expected directions (i.e., positive relationships between reflective cognition and strategic information-seeking behavior or “directed exploration”, and negative relationships between these measures and anxiety/depression symptoms). Further analyses suggested that the relationship between directed exploration and depression/anxiety was due in part to an ambiguity aversion promoting exploration in conditions where information-seeking was not beneficial (as opposed to only being due to under-exploration when more information would aid future choices). In contrast, reflectiveness was associated with greater exploration in appropriate settings and separately accounted for differences in reaction times, decision noise, and choice accuracy in expected directions. These results shed light on the mechanisms underlying information-seeking behavior and how they may contribute to symptoms of emotional disorders. They also highlight the potential clinical relevance of individual differences in reflectiveness and exploration and should motivate future research on their possible contributions to vulnerability and/or maintenance of affective disorders.
Do the Child Welfare and Protection Services Involve Children in Cases With Parental Mental Health Problems? A Norwegian Case-File Study
Authors: Vis Svein Arild , Lauritzen Camilla , Christiansen Øivin , Reedtz Charlotte
Published: January 2022

Background: Parental mental health problems is a common source of concern reported to child welfare and protection services (CWPS). In this study we explored to what extent the child was invited to participate in the investigation process. We aimed to study: (a) what was the current practice in the child protection service in Norway when the CWPS received a report of concern about children whose parents were affected by mental health problems or substance abuse, (b) to what extent were children involved and consulted, (c) which factors predicted the decision to involve the children, and (d) in cases in which conversations with children were conducted: what was the main content of the conversations.

Method: The study was a cross-sectional case file study (N = 1,123). Data were collected retrospectively from case records in 16 different child protection agencies. The cases were randomly drawn from all referrals registered in the participating agencies. Differences in how investigations were conducted in cases with and without concerns about parental mental health were analyzed using t-tests and chi-square testes. Predictors of child involvement in cases with parental mental health problems (N = 324) were estimated by logistic regression analyses.

Results: When the referral to the CWPS contained concerns about parental mental health, there were more consultations with parents, more frequent home visits and the investigation took longer to conclude. The children, however, were less likely to be involved. Children in such cases were consulted in 47.5% of cases. Predictors for involving the children in those cases were child age, concern about the child's emotional problems and if the child was known from previous referrals.

Conclusion: In Norwegian child protection investigations, in which there were concerns about the parent's mental health, conversations with children were conducted to a significantly lower degree compared to cases where the child's problem was the main concern. In such cases, the CWPS workers have to overcome a threshold before they consult with the child. The threshold decreases with child age and when case worker already knows the child.
A Feasibility Study of the WHO Digital Mental Health Intervention Step-by-Step to Address Depression Among Chinese Young Adults
Authors: Sit Hao Fong , Hong Ieng Wai , Burchert Sebastian , Sou Elvo Kuai Long , Wong Mek , Chen Wen , Lam Agnes Iok Fong , Hall Brian J.
Published: January 2022

Background:Chinese young adults experience barriers to mental health treatment, including the lack of treatment providers and stigma around treatment seeking. Evidence-based digital mental health interventions are promising and scalable alternatives to face-to-face treatment for this population, but lack rigorous evidence to support scale-up in China.

Aim:The study was a feasibility study for a large-scale RCT of Step-by-Step, a behavioral activation-based, mental health intervention to address depression and anxiety symptoms in Chinese young adults. It sought to assess feasibility of recruitment and of delivery of Step-by-Step in a University setting, to assess acceptability of the intervention, and to examine potential effectiveness.

Method:An uncontrolled, feasibility trial was conducted to assess the feasibility and acceptability of Chinese Step-by-Step for Chinese University students with elevated depressive symptoms (PHQ-9 scores at or above 10) in Macao, China. Data was collected at two different time points (i.e., baseline and 8-weeks after baseline), administered via questionnaires embedded in an interventional mobile application. Participation rate and dropout rate were measured. Depressive and anxiety symptom severity, well-being, and self-defined stress were assessed. Satisfaction with the program was assessed using qualitative interviews.

Results:A total of 173 students were screened, 22.0% (n= 38) were eligible, and 63.2% of them (n= 24) started the intervention. The dropout rate by post-test was 45.8%. Results from completers showed that Step-by-Step was potentially effective in reducing depressive and anxiety symptom severity, and self-defined stress. Students were generally satisfied with the program, but also offered suggestions for continued improvement. Qualitative feedback was reported within the RE-AIM framework, covering recruitment, effectiveness, adoption, implementation, and maintenance. Amendments to the program were made according to the feedback (e.g., adding notification for new session, modify the time zone).

Conclusion:A minimally guided Step-by-Step protocol and the study procedure were successfully pilot tested for use for Chinese University students. The intervention was acceptable and no adverse events were reported. The results support the potential effectiveness and feasibility of a large-scale evaluation of the program.
Negative Symptom Domains Are Associated With Verbal Learning in Adolescents With Early Onset Psychosis
Authors: Mørch-Johnsen Lynn , Smelror Runar Elle , Andreou Dimitrios , Barth Claudia , Johannessen Cecilie , Wedervang-Resell Kirsten , Wortinger Laura A. , Díaz Ricardo , Victoria Gamaliel , Ueland Torill , Andreassen Ole A. , Myhre Anne M. , Rund Bjørn Rishovd , Ulloa Rosa Elena , Agartz Ingrid
Published: January 2022

Background: Early-onset psychosis (EOP) is among the leading causes of disease burden in adolescents. Negative symptoms and cognitive deficits predicts poorer functional outcome. A better understanding of the association between negative symptoms and cognitive impairment may inform theories on underlying mechanisms and elucidate targets for development of new treatments. Two domains of negative symptoms have been described in adult patients with schizophrenia: apathy and diminished expression, however, the factorial structure of negative symptoms has not been investigated in EOP. We aimed to explore the factorial structure of negative symptoms and investigate associations between cognitive performance and negative symptom domains in adolescents with EOP. We hypothesized that (1) two negative symptom factors would be identifiable, and that (2) diminished expression would be more strongly associated with cognitive performance, similar to adult psychosis patients.

Methods: Adolescent patients with non-affective EOP (n = 169) were included from three cohorts: Youth-TOP, Norway (n = 45), Early-Onset Study, Norway (n = 27) and Adolescent Schizophrenia Study, Mexico (n = 97). An exploratory factor analysis was performed to investigate the underlying structure of negative symptoms (measured with the Positive and Negative Syndrome Scale (PANSS)). Factor-models were further assessed using confirmatory factor analyses. Associations between negative symptom domains and six cognitive domains were assessed using multiple linear regression models controlling for age, sex and cohort. The neurocognitive domains from the MATRICS Consensus Cognitive Battery included: speed of processing, attention, working memory, verbal learning, visual learning, and reasoning and problem solving.

Results: The exploratory factor analysis of PANSS negative symptoms suggested retaining only a single factor, but a forced two factor solution corroborated previously described factors of apathy and diminished expression in adult-onset schizophrenia. Results from confirmatory factor analysis indicated a better fit for the two-factor model than for the one-factor model. For both negative symptom domains, negative symptom scores were inversely associated with verbal learning scores.

Conclusion: The results support the presence of two domains of negative symptoms in EOP; apathy and diminished expression. Future studies on negative symptoms in EOP should examine putative differential effects of these symptom domains. For both domains, negative symptom scores were significantly inversely associated with verbal learning.
Causes of Hospital Violence, Characteristics of Perpetrators, and Prevention and Control Measures: A Case Analysis of 341 Serious Hospital Violence Incidents in China
Authors: Ma Yuanshuo , Wang Licheng , Wang Yongchen , Li Zhe , Zhang Yafeng , Fan Lihua , Ni Xin
Published: January 2022

Objective: Hospital violence remains a global public health problem. This study aims to analyze serious hospital violence causes in China and the characteristics of perpetrators. It likewise seeks to understand frontline personnel's needs and put forward targeted suggestions.

Methods: Serious hospital violence cases from 2011 to 2020 in the China Judgment Online System (CJOS) were selected for descriptive statistical analysis. A total of 72 doctors, nurses, hospital managers, and security personnel from 20 secondary and tertiary hospitals in China were selected for semi-structured interviews.

Results: Of the incidents, 62.17% were caused by patients' deaths and dissatisfaction with their treatment results. Moreover, it was found that out-of-hospital disputes (11.14%) were also one of the main reasons for serious hospital violence. The perpetrators were mainly males (80.3%), and had attained junior high school education or lower (86.5%). Furthermore, most of them were family members of the patients (76.1%). Healthcare workers urgently hope that relevant parties will take new measures in terms of legislation, security, and dispute handling capacity.

Conclusion: In the past 10 years, serious hospital violence's frequency in China has remained high. Furthermore, their harmful consequences are more serious. The causes of hospital violence are diverse, and the characteristics of perpetrators are obvious. Frontline healthcare workers urgently need relevant parties to take effective measures in terms of legislation, security, and dispute handling capacity, to prevent the occurrence of violence and protect medical personnel's safety.
Evidence on Human Exposure to Pesticides and the Occurrence of Health Hazards in the Brazilian Population: A Systematic Review
Authors: Panis Carolina , Kawassaki Aedra Carla Bufalo , Crestani Ana Paula Jaqueline , Pascotto Claudiceia Risso , Bortoloti Durcelina Schiavoni , Vicentini Geraldo Emílio , Lucio Léia Carolina , Ferreira Mariane Okamoto , Prates Rosebel Trindade Cunha , Vieira Valquíria Kulig , Gaboardi Shaiane Carla , Candiotto Luciano Zanetti Pessoa
Published: January 2022

Brazil is among the biggest pesticide consumers in the world, with its population severely exposed to tons of such substances, both because of environmental contamination and occupational use. The health consequences of pesticide exposure are well-documented, but still sparse regarding Brazilian population. This study systematically reviewed the Brazilian studies published that address the relationship between exposure to pesticides and health problems in the Brazilian population. Also, information about pesticide use in Brazil is provided. The included studies showed that exposure to pesticides has a relevant impact on the health of the Brazilian population, regardless of age and gender, and on workers in rural areas or not. Most poisoning events seem to result from the continuous use of pesticides, whether occupationally or environmentally, characterizing a public health problem. The major consequences reported in literature were damage to the central nervous system, cancer, deleterious effects on rural workers' health, intoxications, malformations, and endocrine changes. These findings point out the need to understand the impact of chronic exposure to pesticides on severely exposed people and highlight the importance of creating public policies to protect them and avoid disease occurrence.
Public Knowledge About Emergency Care—Results of a Population Survey From Germany
Authors: von dem Knesebeck Olaf , Koens Sarah , Schäfer Ingmar , Strauß Annette , Klein Jens
Published: January 2022

Background: Knowledge and beliefs about health and health care are part of the general concept of health literacy. Studies demonstrated that large parts of the population report inadequate health literacy. There are only few studies specifically addressing public knowledge and beliefs about emergency care. We examine magnitude and social variations of public knowledge about emergency care in Germany.

Methods: Analyses make use of a telephone survey conducted in Hamburg, Germany. Random sample consisted of 1,207 adult respondents. We asked whether the respondents know various emergency care services. Moreover, capabilities of dealing with an emergency case were assessed. Sex, age, education, and migration background were introduced as predictors into regression models.

Results: 98% of the respondents stated to know the rescue service, while 74% knew the medical on call service and 49% were aware of an emergency practice nearby. About 71% of the interviewees said it was easy for them to find out whom to turn to in a case of a medical emergency. Fewer respondents found it easy to evaluate when to use emergency medical services and to evaluate whether a health problem is a medical emergency. Knowledge and capabilities were positively associated with education and negatively related to migration background.

Conclusions: This study indicates a lack of public knowledge about emergency care and social inequalities in public knowledge according to education and migration status. Findings suggest that interventions are needed to improve public knowledge and that considering social inequalities should be a basic principle for such interventions.
Tactile Sensing for Minimally Invasive Surgery: Conventional Methods and Potential Emerging Tactile Technologies
Authors: Othman Wael , Lai Zhi-Han A. , Abril Carlos , Barajas-Gamboa Juan S. , Corcelles Ricard , Kroh Matthew , Qasaimeh Mohammad A.
Published: January 2022

As opposed to open surgery procedures, minimally invasive surgery (MIS) utilizes small skin incisions to insert a camera and surgical instruments. MIS has numerous advantages such as reduced postoperative pain, shorter hospital stay, faster recovery time, and reduced learning curve for surgical trainees. MIS comprises surgical approaches, including laparoscopic surgery, endoscopic surgery, and robotic-assisted surgery. Despite the advantages that MIS provides to patients and surgeons, it remains limited by the lost sense of touch due to the indirect contact with tissues under operation, especially in robotic-assisted surgery. Surgeons, without haptic feedback, could unintentionally apply excessive forces that may cause tissue damage. Therefore, incorporating tactile sensation into MIS tools has become an interesting research topic. Designing, fabricating, and integrating force sensors onto different locations on the surgical tools are currently under development by several companies and research groups. In this context, electrical force sensing modality, including piezoelectric, resistive, and capacitive sensors, is the most conventionally considered approach to measure the grasping force, manipulation force, torque, and tissue compliance. For instance, piezoelectric sensors exhibit high sensitivity and accuracy, but the drawbacks of thermal sensitivity and the inability to detect static loads constrain their adoption in MIS tools. Optical-based tactile sensing is another conventional approach that facilitates electrically passive force sensing compatible with magnetic resonance imaging. Estimations of applied loadings are calculated from the induced changes in the intensity, wavelength, or phase of light transmitted through optical fibers. Nonetheless, new emerging technologies are also evoking a high potential of contributions to the field of smart surgical tools. The recent development of flexible, highly sensitive tactile microfluidic-based sensors has become an emerging field in tactile sensing, which contributed to wearable electronics and smart-skin applications. Another emerging technology is imaging-based tactile sensing that achieved superior multi-axial force measurements by implementing image sensors with high pixel densities and frame rates to track visual changes on a sensing surface. This article aims to review the literature on MIS tactile sensing technologies in terms of working principles, design requirements, and specifications. Moreover, this work highlights and discusses the promising potential of a few emerging technologies towards establishing low-cost, high-performance MIS force sensing.
Mapping Glacier Ablation With a UAV in the North Cascades: A Structure-from-Motion Approach
Authors: Healy Shannon M. , Khan Alia L.
Published: January 2022

The glaciers of the North Cascades have experienced mass loss and terminus retreat due to climate change. The meltwater from these glaciers provides a flux of cold glacier meltwater into the river systems, which supports salmon spawning during the late summer dry season. The Nooksack Indian Tribe monitors the outlet flow of the Sholes Glacier within the North Cascades range with the goal of understanding the health of the glacier and the ability of the Tribe to continue to harvest sustainable populations of salmon. This study compares the UAV derived glacier ablation with the discharge data collected by the Tribe. We surveyed the Sholes Glacier twice throughout the 2020 melt season and, using Structure-from-Motion technology, generated high resolution multispectral orthomosaics and Digital Elevation Models (DEMs) of the glacier on each of the survey dates. The DEMs were differenced to reveal the surface height change of the glacier. The spectral data of the orthomosaics were used to conduct IsoData unsupervised classification. This process divided the survey area into Snow, Ice, and Rock classes that were then used to attribute the surface height changes of the DEMs to either snow or ice melt. The analysis revealed the glacier lost an average thickness of −0.132 m per day (m d−1) with snow and ice losing thickness at similar rates, −0.130 m d−1 and −0.132 m d−1 respectively. DEM differencing reveals that a total of −550,161 ± 45,206 m3 water equivalent (w.e.) was discharged into Wells Creek between the survey dates whereas the stream gauge station measured a total discharge of 350,023 m3. This study demonstrates the ability to spectrally classify the UAV data and derive discharge measurements while evaluating the small-scale spatial variability of glacier melt. Assessing ablation in small alpine glaciers is of great importance to downstream communities, like the Nooksack Indian Tribe who seek to understand the magnitude and timing of glacier melt in order to better protect their salmon populations. With this paper, we provide a baseline for future glacier monitoring and the potential to connect the snow surface properties with the rate of snow melt into a warming future.
Cross Talk Between Zinc-Solubilizing Bacteria and Plants: A Short Tale of Bacterial-Assisted Zinc Biofortification
Authors: Upadhayay Viabhav Kumar , Singh Ajay Veer , Khan Amir
Published: January 2022

A contemporary approach to bacterially mediated zinc (Zn) biofortification offers a new dimension in the crop improvement program with better Zn uptake in plants to curb Zn malnutrition. The implication of Zn solubilizing bacteria (ZSB) represents an inexpensive and optional strategy for Zn biofortification, with an ultimate green solution to enlivening sustainable agriculture. ZSB dwelling in the rhizospheric hub or internal plant tissues shows their competence to solubilize Zn via a variety of strategies. The admirable method is the deposition of organic acids (OAs), which acidify the surrounding soil environment. The secretion of siderophores as a metal chelating molecule, chelating ligands, and the manifestation of an oxidative–reductive system on the bacterial cell membrane are further tactics of bacterially mediated Zn solubilization. The inoculation of plants with ZSB is probably a more effective tactic for enhanced Zn translocation in various comestible plant parts. ZSB with plant growth-enhancing properties can be used as bioelicitors for sustainable plant growth via the different approaches that are crucial for plant health and its productivity. This article provides an overview of the functional properties of ZSB-mediated Zn localization in the edible portions of food crops and provides an impetus to explore such plant probiotics as natural biofortification agents.
Leveraging Digital Tools and Crowdsourcing Approaches to Generate High-Frequency Data for Diet Quality Monitoring at Population Scale in Rwanda
Authors: Manners Rhys , Adewopo Julius , Niyibituronsa Marguerite , Remans Roseline , Ghosh Aniruddha , Schut Marc , Egoeh Seth Gogo , Kilwenge Regina , Fraenzel Anna
Published: January 2022

Diet quality is a critical determinant of human health and increasingly serves as a key indicator for food system sustainability. However, data on diets are limited, scattered, often project-dependent, and current data collection systems do not support high-frequency or consistent data flows. We piloted in Rwanda a data collection system, powered by the principles of citizen science, to acquire high frequency data on diets. The system was deployed through an unstructured supplementary service data platform, where respondents were invited to answer questions regarding their dietary intake. By combining micro-incentives with a normative nudge, 9,726 responses have been crowdsourced over 8 weeks of data collection. The cost per respondent was < $1 (system set-up, maintenance, and a small payment to respondents), with interactions taking <15 min. Exploratory analyses show that >70% of respondents consume tubers and starchy vegetables, leafy vegetables, fruits, legumes, and wholegrains. Women consumed better quality diets than male respondents, revealing a sex-based disparity in diet quality. Similarly, younger respondents (age ≤ 24 years) consumed the lowest quality diets, which may pose significant risks to their health and mental well-being. Middle-income Rwandans were identified to have consumed the highest quality diets. Long-term tracking of diet quality metrics could help flag populations and locations with high probabilities of nutrition insecurity, in turn guiding relevant interventions to mitigate associated health and social risks.
Quality of Life in Patients With Rectal Resections and End-to-End Primary Anastomosis Using a Standardized Perioperative Pathway
Authors: Herzberg Jonas , Khadem Shahram , Begemann Valentin , Strate Tim , Honarpisheh Human , Guraya Salman Yousuf
Published: January 2022

Objectives: Lower rectal resection is associated with a high rate of postoperative complications and, therefore, adversely impacts the postoperative health-related quality of life (QoL). Though sporadically practiced in different centers, there is no standard perioperative protocol for the management of patients with rectal growths. The aim of this analysis is to evaluate the patient-reported outcomes after low rectal resections followed by an end-to-end-reconstruction and temporary covering ileostomy using a multidisciplinary fail-safe-concept.

Methods: Between 2015 and 2020, we evaluated patient reported outcomes after open and laparoscopic rectal resections with end-to-end reconstruction with a primary straight anastomosis using a standardized perioperative pathway All patients with stoma were excluded from the study. The data for the QoL of patients was collected using the established Low Anterior Resection Syndrome (LARS)-score and the EORTC-C30 and CR-29 questionnaires at a single postoperative timepoint.

Results: We recruited 78 stoma-free patients for this analysis. Of 78 patients included in the study, 87.2% were operated laparoscopically and the mean global health status was 67.95 points, while a major LARS was detected in 48 (61.5%) patients. No anastomotic leakage (AL) occurred within the study cohort. There was no significant change in the LARS-score or the global health status depending on the follow-up-period.

Conclusion: This study shows that good QoL and functional outcomes with no AL are achievable following end-to-end straight anastomosis using a standardized perioperative surgical fail-safe protocol procedure.
The First Lumpy Skin Disease Outbreak in Thailand (2021): Epidemiological Features and Spatio-Temporal Analysis
Authors: Arjkumpa Orapun , Suwannaboon Minta , Boonrod Manoch , Punyawan Issara , Liangchaisiri Supawadee , Laobannue Patchariya , Lapchareonwong Chayanun , Sansri Chaiwat , Kuatako Noppasorn , Panyasomboonying Pawares , Uttarak Ponkrit , Buamithup Noppawan , Sansamur Chalutwan , Punyapornwithaya Veerasak
Published: January 2022

The first outbreak of lumpy skin disease (LSD) in Thailand was reported in March 2021, but information on the epidemiological characteristics of the outbreak is very limited. The objectives of this study were to describe the epidemiological features of LSD outbreaks and to identify the outbreak spatio-temporal clusters. The LSD-affected farms located in Roi Et province were investigated by veterinary authorities under the outbreak response program. A designed questionnaire was used to obtain the data. Space-time permutation (STP) and Poisson space-time (Poisson ST) models were used to detect areas of high LSD incidence. The authorities identified 293 LSD outbreak farms located in four different districts during the period of March and the first week of April 2021. The overall morbidity and mortality of the affected cattle were 40.5 and 1.2%, respectively. The STP defined seven statistically significant clusters whereas only one cluster was identified by the Poisson ST model. Most of the clusters (n = 6) from the STP had a radius <7 km, and the number of LSD cases in those clusters varied in range of 3–51. On the other hand, the most likely cluster from the Poisson ST included LSD cases (n = 361) from 198 cattle farms with a radius of 17.07 km. This is the first report to provide an epidemiological overview and determine spatio-temporal clusters of the first LSD outbreak in cattle farms in Thailand. The findings from this study may serve as a baseline information for future epidemiological studies and support authorities to establish effective control programs for LSD in Thailand.
ABCC9 Is Downregulated and Prone to Microsatellite Instability on ABCC9tetra in Canine Breast Cancer
Authors: Hao Pan , Song Kai-yue , Wang Si-qi , Huang Xiao-jun , Yao Da-wei , Yang De-ji
Published: January 2022

Tumorigenesis is associated with metabolic abnormalities and genomic instability. Microsatellite mutations, including microsatellite instability (MSI) and loss of heterozygosity (LOH), are associated with the functional impairment of some tumor-related genes. To investigate the role of MSI and LOH in sporadic breast tumors in canines, 22 tumors DNA samples and their adjacent normal tissues were evaluated using polyacrylamide gel electrophoresis and silver staining for 58 microsatellites. Quantitative real-time polymerase chain reaction, promoter methylation analysis and immunohistochemical staining were used to quantify gene expression. The results revealed that a total of 14 tumors (6 benign tumors and 8 breast cancers) exhibited instability as MSI-Low tumors. Most of the microsatellite loci possessed a single occurrence of mutations. The maximum number of MSI mutations on loci was observed in tumors with a lower degree of differentiation. Among the unstable markers, FH2060 (4/22), ABCC9tetra (4/22) and SCN11A (6/22) were high-frequency mutation sites, whereas FH2060 was a high-frequency LOH site (4/22). The ABCC9tetra locus was mutated only in cancerous tissue, although it was excluded by transcription. The corresponding genes and proteins were significantly downregulated in malignant tissues, particularly in tumors with MSI. Furthermore, the promoter methylation results of the adenosine triphosphate binding cassette subfamily C member 9 (ABCC9) showed that there was a high level of methylation in breast tissues, but only one case showed a significant elevation compared with the control. In conclusion, MSI-Low or MSI-Stable is characteristic of most sporadic mammary tumors. Genes associated with tumorigenesis are more likely to develop MSI. ABCC9 protein and transcription abnormalities may be associated with ABCC9tetra instability.
Development of Sliding Mode Controller Based on Internal Model Controller for Higher Precision Electro-Optical Tracking System
Authors: Zhang Bing , Nie Kang , Chen Xinglong , Mao Yao
Published: January 2022

The electro-optical tracking system (ETS) on moving platforms is affected by the vibration of the moving carrier, the wind resistance torque in motion, the uncertainty of mechanisms and the nonlinear friction between frames and other disturbances, which may lead to the instability of the electro-optical tracking platform. Sliding mode control (SMC) has strong robustness to system disturbances and unknown dynamic external signals, which can enhance the disturbance suppression ability of ETSs. However, the strong robustness of SMC requires greater switching gain, which causes serious chattering. At the same time, the tracking accuracy of SMC has room for further improvement. Therefore, in order to solve the chattering problem of SMC and improve the tracking accuracy of SMC, an SMC controller based on internal model control (IMC) is proposed. Compared with traditional SMC, the proposed method can be used to suppress the strongest disturbance with the smallest switching gain, effectively solving the chattering problem of the SMC, while improving the tracking accuracy of the system. In addition, to reduce the adverse influence of sensor noise on the control effect, lifting wavelet threshold de-noising is introduced into the control structure to further improve the tracking accuracy of the system. The simulation and experimental results verify the superiority of the proposed control method.
Combining Random Forest and XGBoost Methods in Detecting Early and Mid-Term Winter Wheat Stripe Rust Using Canopy Level Hyperspectral Measurements
Authors: Huang Linsheng , Liu Yong , Huang Wenjiang , Dong Yingying , Ma Huiqin , Wu Kang , Guo Anting
Published: January 2022

Appropriate modeling methods and feature selection algorithms must be selected to improve the accuracy of early and mid-term remote sensing detection of wheat stripe rust. In the current study, we explored the effectiveness of the random forest (RF) algorithm combined with the extreme gradient boosting (XGboost) method for early and mid-term wheat stripe rust detection based on the vegetation indices extracted from canopy level hyperspectral measurements. Initially, 21 vegetation indices that were related to the early and mid-term winter wheat stripe rust were calculated on the basis of canopy level hyperspectral reflectance. Subsequently, the optimal vegetation index combination for disease detection was determined using correlation analysis (CA) combined with RF algorithms. Then, the disease severity detection model of early and mid-term winter wheat stripe rust was constructed using XGBoost method based on the optimal vegetation index combination. For the evaluation and comparison of the initial results, three commonly used classification methods, namely, RF, backpropagation neural network (BPNN), and support vector machine (SVM), were utilized. The vegetation index combinations determined by the single CA algorithm were also used to construct detection models. Compared with the detection models based on the vegetation index combination obtained using the single CA algorithm, the overall accuracy of the four detection models based on the optimal vegetation index combination based on CA combined with RF algorithms increased by 16.1% (XGBoost), 9.7% (RF), 8.1% (SVM), and 8.1% (BPNN). Among the eight models, the XGBoost detection model based on the optimal vegetation index combination using CA combined with RF algorithms, CA-RF-XGBoost, achieved the highest overall accuracy of 87.1% and the highest kappa coefficient of 0.798. Our results indicate that the RF combined with XGBoost can improve the detection accuracy of early and mid-term winter wheat stripe rust effectively at canopy scale.
A Review of the Biology, Ecology, and Management of the South American Locust, Schistocerca cancellata (Serville, 1838), and Future Prospects
Authors: Trumper Eduardo V. , Cease Arianne J. , Cigliano María Marta , Copa Bazán Fernando , Lange Carlos E. , Medina Héctor E. , Overson Rick P. , Therville Clara , Pocco Martina E. , Piou Cyril , Zagaglia Gustavo , Hunter David
Published: January 2022

In the first half of the twentieth century, the South American Locust (SAL), Schistocerca cancellata (Serville, 1838), was a major pest of agriculture in Argentina, Bolivia, Paraguay, Uruguay, and Brazil. From 1954–2014, a preventive management program appeared to limit SAL populations, with only small- to moderate-scale treatments required, limited to outbreak areas in northwest Argentina. However, the lack of major locust outbreaks led to a gradual reduction in resources, and in 2015, the sudden appearance of swarms marked the beginning of a substantial upsurge, with many swarms reported initially in Argentina in 2015, followed by expansion into neighboring countries over the next few years. The upsurge required a rapid allocation of resources for management of SAL and a detailed examination of the improvements needed for the successful management of this species. This paper provides a review of SAL biology, management history, and perspectives on navigating a plague period after a 60-year recession.
Crop Yield and Soil Quality Are Partners in a Sustainable Agricultural System
Authors: Papatheodorou Efimia M. , Monokrousos Nikolaos
Published: January 2022

Agricultural practices involving the excessive use of chemical fertilizers and pesticides pose major risks to the environment and human health [...]
Role of AmpC-Inducing Genes in Modulating Other Serine Beta-Lactamases in Escherichia coli
Authors: Mallik Dhriti , Jain Diamond , Bhakta Sanjib , Ghosh Anindya Sundar
Published: January 2022

The consistently mutating bacterial genotypes appear to have accelerated the global challenge with antimicrobial resistance (AMR); it is therefore timely to investigate certain less-explored fields of targeting AMR mechanisms in bacterial pathogens. One of such areas is beta-lactamase (BLA) induction that can provide us with a collection of prospective therapeutic targets. The key genes (ampD, ampE and ampG) to which the AmpC induction mechanism is linked are also involved in regulating the production of fragmented muropeptides generated during cell-wall peptidoglycan recycling. Although the involvement of these genes in inducing class C BLAs is apparent, their effect on serine beta-lactamase (serine-BLA) induction is little known. Here, by using ∆ampD and ∆ampE mutants of E. coli, we attempted to elucidate the effects of ampD and ampE on the expression of serine-BLAs originating from Enterobacteriaceae, viz., CTX-M-15, TEM-1 and OXA-2. Results show that cefotaxime is the preferred inducer for CTX-M-15 and amoxicillin for TEM-1, whereas oxacillin for OXA-2. Surprisingly, exogenous BLA expressions are elevated in ∆ampD and ∆ampE mutants but do not always alter their beta-lactam susceptibility. Moreover, the beta-lactam resistance is increased upon in trans expression of ampD, whereas the same is decreased upon ampE expression, indicating a differential effect of ampD and ampE overexpression. In a nutshell, depending on the BLA, AmpD amidase moderately facilitates a varying level of serine-BLA expression whereas AmpE transporter acts likely as a negative regulator of serine-BLA.
The Beneficial Effects of Heme Oxygenase 1 and Hydrogen Sulfide Activation in the Management of Neuropathic Pain, Anxiety- and Depressive-like Effects of Paclitaxel in Mice
Authors: Roch Gerard , Batallé Gerard , Bai Xue , Pouso-Vázquez Enric , Rodríguez Laura , Pol Olga
Published: January 2022

Chemotherapy-induced peripheral neuropathy constitutes an unresolved clinical problem that severely decreases the quality of the patient’s life. It is characterized by somatosensory alterations, including chronic pain, and a high risk of suffering mental disorders such as depression and anxiety. Unfortunately, an effective treatment for this neuropathology is yet to be found. We investigated the therapeutic potential of cobalt protoporphyrin IX (CoPP), a heme oxygenase 1 inducer, and morpholin-4-ium 4-methoxyphenyl(morpholino) phosphinodithioate dichloromethane complex (GYY4137), a slow hydrogen sulfide (H2S) donor, in a preclinical model of paclitaxel (PTX)-induced peripheral neuropathy (PIPN) in mice. At three weeks after PTX injection, we evaluated the effects of the repetitive administration of 5 mg/kg of CoPP and 35 mg/kg of GYY4137 on PTX-induced nociceptive symptoms (mechanical and cold allodynia) and on the associated emotional disturbances (anxiety- and depressive-like behaviors). We also studied the mechanisms that could mediate their therapeutic properties by evaluating the expression of key proteins implicated in the development of nociception, oxidative stress, microglial activation, and apoptosis in prefrontal cortex (PFC) and dorsal root ganglia (DRG) of mice with PIPN. Results demonstrate that CoPP and GYY4137 treatments inhibited both the nociceptive symptomatology and the derived emotional alterations. These actions were mainly mediated through potentiation of antioxidant responses and inhibiting oxidative stress in the DRG and/or PFC of mice with PIPN. Both treatments normalized some plasticity changes and apoptotic reactions, and GYY4137 blocked microglial activation induced by PTX in PFC. In conclusion, this study proposes CoPP and GYY4137 as good candidates for treating neuropathic pain, anxiety- and depressive-like effects of PTX.
Meso-Dihydroguaiaretic Acid Ameliorates Acute Respiratory Distress Syndrome through Inhibiting Neutrophilic Inflammation and Scavenging Free Radical
Authors: Lee Yen-Tung , Chen Yu-Li , Wu Yi-Hsuan , Chen Ih-Sheng , Chang Hsun-Shuo , Wang Yi-Hsuan , Chang Shih-Hsin , Wu Yi-Hsiu , Kao Ting-I , Yu Huang-Ping , Hwang Tsong-Long
Published: January 2022

The pathogenesis of acute respiratory distress syndrome (ARDS) is very complex. Patients with ARDS still suffer high mortality rates. Infiltration and activation of neutrophils in lungs are critical pathogenic factors in ARDS. In this study, we demonstrate that meso-dihydroguaiaretic acid (MDGA), a natural lignan, inhibits inflammatory responses in human neutrophils and ameliorates ARDS in mice. MDGA inhibited superoxide anion generation and elastase release in various G-protein coupled receptor agonists-induced human neutrophils. However, MDGA did not alter superoxide anion generation and elastase activity in cell-free systems. These results suggest that the anti-inflammatory effects of MDGA are mediated by regulating cellular signals in human neutrophils. In consistent with this, MDGA suppressed phosphorylation of extracellular signal-regulated kinase and c-Jun N-terminal kinase in activated human neutrophils. Moreover, MDGA inhibited CD11b expression and adhesion in activated human neutrophils. Interestingly, MDGA reduced reactive oxygen species (ROS) generation but not superoxide anion generation in protein kinase C (PKC) activator-induced human neutrophils, suggesting that MDGA may also have ROS scavenging ability. Indeed, MDGA showed strong free radical scavenging activity in cell-free assays. Significantly, MDGA suppressed PKC-induced neutrophil extracellular trap formation. Additionally, treatment of MDGA attenuated neutrophil infiltration and lung damage on lipopolysaccharide-induced ARDS in mice. In conclusion, our results demonstrate that MDGA has anti-neutrophilic inflammatory effects and free-radical scavenging activity. We also suggest that MDGA has potential to serve as a lead for developing new therapeutics to treat ARDS.
Polymorphism of Transferrin Gene Impacts the Mediating Effects of Psychotic Symptoms on the Relationship between Oxidative Stress and Cognition in Patients with Chronic Schizophrenia
Authors: Chen Pinhong , Wang Dongmei , Xiu Meihong , Chen Dachun , Lackey Blake , Wu Hanjing E. , Zhou Jin , Zhang Xiangyang
Published: January 2022

A series of studies indicated that iron distribution that partly derives from transferrin-bound iron in the peripheral nervous system in the brain may act in processes such as myelination and brain development. However, the relationship between schizophrenia, its psychotic symptoms, and the transferrin (TF) gene has not been systematically explored. Our study aimed to investigate how a particular polymorphism of the transferrin gene, rs3811655, affects the superoxide dismutase (SOD), malondialdehyde (MDA), psychotic symptoms, cognition, or the mediation model between antioxidant enzymes and cognition via symptoms. A total of 564 patients with chronic schizophrenia and 468 healthy control subjects were recruited. The psychotic symptoms and cognition were assessed by the Positive and Negative Syndrome Scale (PANSS) and the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), respectively. Furthermore, the serum SOD, MDA activity, and transferrin gene polymorphism were measured in patients. Our results demonstrated that patients with the G allele possessed more severe negative symptoms, worse cognitive performance with respect to attention, and higher serum Mn-SOD activity. Additionally, the rs3811655 polymorphism may act as a moderator in the association between Cu/Zn-SOD activity and cognition, as well as psychotic symptoms in patients suffering from schizophrenia. According to this study, the single nucleotide polymorphism (SNP) rs3811655 polymorphism may fail to contribute to the susceptibility of schizophrenia in an individual but is involved in the iron-induced oxidative stress disturbance and cognitive impairment in schizophrenia. This deepens our understanding of the critical role of iron-induced oxidative stress that might underlie the pathophysiology of schizophrenia.
The Role of Peroxiredoxins in the Regulation of Sepsis
Authors: Aki Toshihiko , Unuma Kana , Uemura Koichi
Published: January 2022

Oxidative stress, a result of a disturbance in redox homeostasis, is considered to be one of the main aggravating events in the pathogenesis of immune disorders. Peroxiredoxins (Prdxs) are an enzyme family that catalyzes the reduction of peroxides, including hydrogen peroxide, lipid peroxides, and nitrogen peroxides. Although the maintenance of cellular redox homeostasis through Prdxs is essential for surviving in adverse environments, Prdxs also participate in the regulation of cellular signal transduction by modulating the activities of a panel of molecules involved in the signal transduction process. Although Prdxs were discovered as intracellular anti-oxidative enzymes, recent research has revealed that Prdxs also play important roles in the extracellular milieu. Indeed, Prdxs have been shown to have the capacity to activate immune cells through ligation with innate immune receptors such as toll-like receptors (TLRs). In this review, we will summarize the intracellular as well as extracellular roles of Prdxs for and against the pathogenesis of inflammatory disorders including sepsis, hemorrhagic shock, and drug-induced liver injury.