bluuebunny/crossref_metadata_2025 huggingface dataset:
Does China’s National Demonstration Eco-Industrial Park Reduce Carbon Dioxide and Sulfur Dioxide—A Study Based on the Upgrading and Transformation Process
Authors: Cao Kairui , Jin Laiqun , Zhu Yuanyuan , Nie Zilong , Li Hao
Published: October 2022

With the increasingly serious environmental problems, coordinating the relationship between the environment and economic development has become a crucial task for developing countries, especially China. This paper studies the role of eco-industrial parks (EIPs) in the emissions of carbon dioxide and sulfur dioxide in China with the difference-in-difference (DID) approach by focusing on the entire process of EIPs’ establishment—upgrading provincial development zones (DZs) to national DZs and then transforming national DZs into EIPs. Besides, we examined the heterogeneous effect of the different transformations from national economic and technological development zones (ETZs) or national high-tech zones (HTZs) to EIPs. In addition, we studied the spatial spillover effects of EIPs and their paths with the spatial difference-in-difference (SDID) method. The results show that neither provincial DZs nor national DZs can significantly reduce sulfur dioxide and carbon dioxide emissions. Only when national DZs are transformed into EIPs can they be reduced significantly. However, the different transformations from the HTZs and ETZs to EIPs have different effects on emissions. Moreover, EIPs have technology spillovers and demonstration effects on surrounding areas. Therefore, EIPs can reduce emissions in the surrounding areas. The results indicate that, in order to achieve high-quality development and coordinate the relationship between environment and economic development, we should take positive steps to promote the transformation of DZs into EIPs.
Exploring the Need for Mobile Application in Stroke Management by Informal Caregivers: A Qualitative Study
Authors: Haji Mukhti Muhammad Iqbal , Ibrahim Mohd Ismail , Tengku Ismail Tengku Alina , Nadal Iliatha Papachristou , Kamalakannan Sureshkumar , Kinra Sanjay , Abdullah Jafri Malin , Musa Kamarul Imran
Published: October 2022

Background: Mobile health (mHealth) has been considered as a prominent concept in digital health and is widely used and easily accessible. Periodic follow-up visits, previously planned procedures, and rehabilitation services for stroke survivors have been cut down during the recent COVID-19 pandemic. Therefore, in this qualitative study we aimed to explore the need for a mobile application in stroke management by informal caregivers. Methods: A phenomenological qualitative study was conducted from November 2020 to June 2021. Thirteen respondents were recruited from two public rehabilitation centers in Kota Bharu, Kelantan, Malaysia. In-depth interviews were conducted. A comprehensive representation of perspectives from the respondents was achieved through purposive sampling. The interviews were conducted in the Kelantanese dialect, recorded, transcribed, and analyzed by using thematic analysis. Results: Thirteen participants were involved in the interviews. All of them agreed with the need for a mobile application in stroke management. They believed the future stroke application will help them to seek information, continuous stroke home care, and help in the welfare of caregivers and stroke patients. Conclusions: The current study revealed two themes with respective subthemes that were identified, namely, self-seeking for information and reasons for using a stroke mobile application in the future. This application helps in reducing healthcare costs, enhancing the rehabilitation process, facilitating patient engagement in decision making, and the continuous monitoring of patient health.
The Impact of Transportation Restructuring on the Intensity of Greenhouse Gas Emissions: Empirical Data from China
Authors: Wang Huiling , Luo Jiaxin , Zhang Mengtian , Ling Yue
Published: October 2022

Adjusting transportation structure to reduce the intensity of greenhouse gas emissions is an effective way to address climate change issues. This paper selects six transport sectors and constructs a hybrid input-output model to study the impact of transportation restructuring on the intensity of CO2 and non-CO2 greenhouse gas emissions in each sector during different periods. The results show that the effect of transportation restructuring on greenhouse gas emissions is manifested differently in different time periods. After 2008, transportation restructuring had a significant effect on reducing the intensity of greenhouse gas emissions in all sectors. However, the impact of transportation restructuring on the intensity of non-CO2 greenhouse gas emissions is limited. It is also found that the railway transport sector has been a low-impact transport sector in terms of greenhouse gas emissions since 2004, which provides insights for the optimization of China’s transportation structure.
Causes of Stress among Healthcare Professionals and Successful Hospital Management Approaches to Mitigate It during the COVID-19 Pandemic: A Cross-Sectional Study
Authors: Herraiz-Recuenco Lourdes , Alonso-Martínez Laura , Hannich-Schneider Susanne , Puente-Alcaraz Jesús
Published: October 2022

The outbreak of the COVID-19 pandemic posed an immediate challenge to the management of hospitals in Germany and elsewhere. The risk of stress for front-line healthcare professionals forced occupational health and safety units to adopt a variety of protective measures, not all of which have been thoroughly validated. The main objective of the present analysis is to assess what the most important sources of stress were and which of the protective measures applied to counteract stress among healthcare staff had the greatest impact. A better understanding of these factors will improve hospital management and worker safety in a future health crisis situation and may also prove to be beneficial in non-crisis situations. For this purpose, in 2020, an exploratory, cross-sectional and quantitative study using a questionnaire created for this purpose was carried out on a total of 198 professionals—133 nurses and 65 physicians—at the Klinikum Mittelbaden Balg hospital in Baden-Baden, Germany, during the first wave of the pandemic. Statistical analyses showed that nurses suffer more stress than physicians and that stress is higher among professionals in critical care and emergency units than in units that are less exposed to infected patients. It was also found that measures such as salary incentives, encouragement of work in well-integrated teams, and perceived support from hospital management mitigate stress. These findings highlight the importance of support measures from management and superiors. Knowing the actual effectiveness of the measures applied by management and the factors mentioned above could help to protect healthcare professionals in the event of another pandemic or similar situations and may still be of value in dealing with the continuing COVID-19 pandemic.
Physical Activity and Executive Functions in Adolescents: The Mediating Role of Sleepiness
Authors: Sun Fenghua , Zhang Fan , Ho Karen Ying-Fung , Zhang Borui , Wang Zixin , Tse Andy Choi-Yeung
Published: October 2022

(1) Background: Both physical activity and sleepiness were found to influence the development of executive functioning. The present study aimed to address the effects of different levels of physical activity on adolescents’ executive performance (i.e., working memory and inhibition), and the role of sleepiness in this relationship. It was hypothesized that a higher level of physical activity would be associated with better executive functioning, while this relationship was mediated (at least partially) by reduced sleepiness at school. (2) Methods: 212 adolescents aged from 10 to 17 were recruited, and they were requested to wear accelerometers for seven consecutive days to measure daily levels of physical activity. The Cleveland Adolescent Sleepiness questionnaire (CASQ) was used to assess sleepiness. The working memory and inhibition control were assessed to indicate executive functioning. (3) Results: It was found that sedentary activity was negatively associated with working memory performance, while light and moderate-to-vigorous physical activities were related to better working memory. The relationship between different levels of physical activity and working memory was mediated by reduced sleepiness at school. (4) Conclusions: Our findings provide nuanced evidence that the benefits of light and moderate-to-vigorous physical activity on cognitive development could be explained by reduced sleepiness at school.
Types of Community Support Services and Self-Efficacy for Continuous Community Living among Individuals with Disabilities and Caregivers
Authors: Chan Wai , Cao Yuan , Lu Erin Yiqing , Cheung Wai Ming , Tsang Hector Wing Hong
Published: October 2022

This study explored the impacts of five types of community support services (i.e., center-based care, home-based care, respite care, caregiver assistance, and financial subsidies) on self-efficacy for continuous community living among individuals with disabilities and caregivers. Design: Cross-sectional. Method: The sample consisted of a group of individuals with disabilities (n = 948) and a group of caregivers (n = 522). A mixed ANOVA was applied to explore the differences in the perceived importance of improvements to community support services between the groups. Logistic regression analyses were conducted to examine the perceived importance of improvements to types of community support services for self-efficacy for continuous community living. Results: Caregivers perceived higher levels of importance for improvements to community support services than individuals with disabilities. Both groups reported that financial subsidies were the most important area for improvement. The greater importance of improvements to financial subsidies reported by caregivers predicted greater odds for self-efficacy for continuous community living. The greater importance of improvements to center-based services reported by individuals with disabilities predicted greater odds for self-efficacy for continuous community living. Conclusions: The findings suggested that financial subsidies for caregivers and center-based services for individuals with disabilities could improve self-efficacy for continuous community living.
Association between Primary Care Utilization and Emergency Room or Hospital Inpatient Services Utilization among the Middle-Aged and Elderly in a Self-Referral System: Evidence from the China Health and Retirement Longitudinal Study 2011–2018
Authors: Yang Siman , Zhou Mengping , Liao Jingyi , Ding Xinxin , Hu Nan , Kuang Li
Published: October 2022

With rapid economic growth and aging, hospital inpatient and emergency services utilization has grown rapidly, and has emphasized an urgent requirement to adjust and optimize the structure of health service utilization. Studies have shown that primary care is an effective way to reduce inpatient and emergency room (ER) service utilization. This study aims to examine whether middle-aged and elderly individuals who selected primary care outpatient services in the last month had less ER and hospital inpatient service utilization than those who selected hospitals outpatient services via the self-referral system. Data were obtained from four waves of the nationally representative China Health and Retirement Longitudinal Study (CHARLS). We pooled respondents who had outpatient visits and were aged 45 years and above. We used logistic regressions to explore the association between types of outpatient and ER visits or hospitalization, and then used zero-truncated negative binomial regression to examine the impact of outpatient visit types on the number of hospitalizations and the length of hospitalization days. A trend test was used to explore the trend of outpatient visit types and the ER or hospital inpatient services utilization with the increase in outpatient visits. Among the 7544 respondents in CHARLS, those with primary care outpatient visits were less likely to have ER visits (adjusted OR = 0.141, 95% CI: 0.101–0.194), hospitalization (adjusted OR = 0.623, 95% CI: 0.546–0.711), and had fewer hospitalization days (adjusted IRR = 0.886, 95% CI: 0.81–0.969). The trend test showed that an increase in the number of total outpatient visits was associated with a lower hospitalizations (p = 0.006), but a higher odds of ER visits (p = 0.023). Our findings suggest that policy makers need to adopt systematic policies that focus on restructuring and balancing the structure of resources and service utilization in the three-tier healthcare system.
Impact of Non-Face-to-Face Teaching with Passive Training on Personal Protective Equipment Use in Health Science Students: A Randomized Controlled Trial
Authors: Rueda-Medina Blanca , Aguilar-Ferrándiz María Encarnación , Esteban-Burgos Ana Alejandra , Tapia Haro Rosa María , Casas-Barragán Antonio , Velando-Soriano Almudena , Gil-Gutiérrez Rocío , Correa-Rodríguez María
Published: October 2022

Background: In the COVID-19 era, there was a call for the transformation of higher education. Universities had to combine non-face-to-face teaching with traditional procedures. This study analyzed the effectiveness and perceived satisfaction in a cohort of health sciences students of non-face-to-face teaching with passive training versus face-to-face teaching with active training in the proper donning and doffing of personal protective equipment (PPE) in a clinical simulation scenario. Methods: A total of 142 participants were randomized into two groups: (a) non-face-to-face teaching with passive training; (b) face-to-face teaching with active training. The proper protocol for donning and doffing PPE was assessed. Students evaluated their skills before and after training and satisfaction with training received. Results: Significant differences were observed for the statements “I felt more confident in donning after receiving this training” (p = 0.029) and “I felt more confident in doffing after receiving this training” (p = 0.042) in the face-to-face teaching with active training group compared to the non-face-to-face teaching with passive training group, whose number of tasks violated was significantly higher (p = 0.020). Satisfaction was significantly higher in the face-to-face and active training group (p = 0.004). Conclusions: Face-to-face teaching with active training improves effectiveness and satisfaction more than non-face-to-face teaching with passive training for acquiring skills in donning and doffing PPE properly.
Empowerment for the Digital Transformation: Results of a Structured Blended-Learning On-the-Job Training for Practicing Physicians in Germany
Authors: Bosch Josefin , Ludwig Christiane , Fluch-Niebuhr Johannes , Stoevesandt Dietrich
Published: October 2022

(1) Background: Practicing physicians have not been in the focus of structured qualifications in basic digital competences so far. However, they are the current gatekeepers to implement digital technologies and need empowerment to proactively take part in the ongoing digital transformation process. The present study investigates if a structured blended-learning training for practicing physicians in Germany enhances both physicians’ knowledge about central aspects of the digital transformation (including awareness of personal possibilities to act) and their attitudes towards a more digitally empowered mindset. (2) Methods: Participants (n = 32) self-assessed their knowledge (19 items, 10-point Likert-scale) and attitudes (6 items, 5-point Likert-scale) towards the digital transformation at the beginning and at the end of the training. MANCOVAs were conducted. (3) Results: Participants reported an increase in every knowledge domain, representing large effects (Hedges’ g 1.06 to 2.82). Attitudes were partly shifted towards a more empowered mindset with decreased insecurity towards technological, legal, and ethical aspects of the digital transformation (Hedges’ g −0.82 to −1.40). However, preparedness for the digital transformation remained low. (4) Conclusions: Generally, the hypotheses were confirmed. The presented on-the-job training had the desired effects on practicing physicians’ knowledge and attitudes. Nevertheless, additional empowerment and support are essential.
Workplace Mistreatment and Health Conditions Prior and during the COVID-19 in South Korea: A Cross-Sectional Study
Authors: Nerobkova Nataliya , Kim Soo Young , Park Eun-Cheol , Shin Jaeyong
Published: October 2022

Background: This study examined the relationship between workplace mistreatment, including discrimination, abuse, and overworking, and health problems among full-time workers prior to and during the coronavirus disease (COVID-19) outbreak in South Korea. Methods: We analyzed data from the 2017 and 2020–2021 Korean Working Conditions Surveys, including the final sample of 44,425 participants. Multiple logistic regression was used to examine the relationship between workplace mistreatment and health problems among workers by gender. Interaction analysis was conducted to establish the association between the COVID-19 pandemic and health problems related to mistreatment. The occupational, demographic, and socioeconomic backgrounds were adjusted. Results: We found a significant association between workplace mistreatment and health problems, including headaches, eyestrain, and anxiety. The association increased after the COVID-19 pandemic: “discrimination” (men (OR 2.26, 95% CI 1.93–2.65), women (OR 2.73, 95% CI 2.36–3.17)); abuse (men (OR 5.42, 95% CI 2.87–10.23), women (OR 4.70, 95% CI 3.12–7.08)); and overworking: men (OR 2.36, 95% CI 2.01–2.77), women (OR 3.52, 95% CI 2.68–4.61). The interaction indicates an increased incidence of people having health problems due to workplace mistreatment (OR 1.03, 95% CI 1.00–1.06) during the COVID-19 pandemic. Conclusion: Statistically, employees who experience workplace mistreatment have worse health conditions. The COVID-19 pandemic has affected the job environment and increased the association between workplace mistreatment and health problems. To eliminate the health problems related to workplace mistreatment, it is necessary to address the impact of the COVID-19 pandemic on work and employee health conditions.
Knowledge, Attitudes, and Practices Survey among Nursing Care Workers Involved in Caring for Older Adults during the Early Stage of the COVID-19 Pandemic in Japan
Authors: Kambayashi Dan , Manabe Toshie , Hirohara Masayoshi , Akatsu Hiroyasu
Published: October 2022

Background: As Japan undergoes population aging, nursing care workers play an important role in supporting older adults in the community, which has been particularly critical during COVID-19 pandemic. However, the knowledge, attitudes, and practices (KAP) among nursing care workers regarding COVID-19 have not been fully elucidated. Methods: A self-administered questionnaire survey was conducted in June 2020 among 481 nursing care workers in the nursing care facilities in Aichi, Japan. We assessed COVID-19-related KAP scores of nursing care workers, and compared them by age, sex, and years of experience. Results: A total of 481 nursing care workers responded to the survey. Out of a maximum of 10 points, the mean (standard deviations) knowledge, attitude, and practice scores were 6.86 (1.45), 7.11 (1.42), and 7.40 (1.89), respectively. Comparisons between the KAP scores revealed significantly higher knowledge scores among older workers (p < 0.001) and significantly higher knowledge scores (p = 0.002) and practice scores (p = 0.033) among workers with more than 20 years of working experience. Conclusions: The findings revealed that older age and a longer duration of experience were associated with higher COVID-19-related knowledge and practice scores. To better support older adults, it is essential to improve the education system for care workers and to provide environments for delivering necessary information rapidly.
Being Naked - et Quo hinc?: Developing a ‘Skin-in-the-Game’ Solution for Credit Default Swaps
Authors: Senarath Shanuka , Rajapakse Pelma , Robbé Jan Job de Vries , Wickremeratne Naveen , Subasinghage Maduka
Published: October 2022

A credit default swap (CDS) is a derivative financial instrument that provides insurance against credit risk. CDSs on subprime Asset Backed Securities (ABSs) paved the way for securitizers to hedge the credit risk of the underlying subprime loans during the onset of the Global Financial Crisis (GFC). Thus, mortgage originators were least concerned about the quality of loans they securitize since they could hedge the default risk via CDS, paving way to a moral hazard concern. We argue that the core issue pertaining to CDSs, moral hazards, remains unattended even after a decade since the GFC. This paper, utilizing a lexonomic approach embedded in the second-best efficiency criteria, examines the mechanism behind a CDS and develops a regulatory framework with the view of minimizing moral hazards associated with CDSs. Our analysis indicates that incorporating an ‘excess’ on CDSs may minimize moral hazards, since originators are compelled to bear part of the risk associated with assets they create.
Transcriptome Profiling of the Hippocampal Seizure Network Implicates a Role for Wnt Signaling during Epileptogenesis in a Mouse Model of Temporal Lobe Epilepsy
Authors: Mardones Muriel D. , Gupta Kunal
Published: October 2022

Mesial temporal lobe epilepsy (mTLE) is a life-threatening condition characterized by recurrent hippocampal seizures. mTLE can develop after exposure to risk factors such as febrile seizure, trauma, and infection. Within the latent period between exposure and onset of epilepsy, pathological remodeling events occur that contribute to epileptogenesis. The molecular mechanisms responsible are currently unclear. We used the mouse intrahippocampal kainite model of mTLE to investigate transcriptional dysregulation in the ipsilateral and contralateral dentate gyrus (DG), representing the epileptogenic zone (EZ) and peri-ictal zone (PIZ). DG were analyzed after 3, 7, and 14 days by RNA sequencing. In both the EZ and PIZ, transcriptional dysregulation was dynamic over the epileptogenic period with early expression of genes representing cell signaling, migration, and proliferation. Canonical Wnt signaling was upregulated in the EZ and PIZ at 3 days. Expression of inflammatory genes differed between the EZ and PIZ, with early expression after 3 days in the PIZ and delayed expression after 7–14 days in the EZ. This suggests that critical gene changes occur early in the hippocampal seizure network and that Wnt signaling may play a role within the latent epileptogenic period. These findings may help to identify novel therapeutic targets that could prevent epileptogenesis.
α-CAs from Photosynthetic Organisms
Authors: Langella Emma , Di Fiore Anna , Alterio Vincenzo , Monti Simona Maria , De Simone Giuseppina , D’Ambrosio Katia
Published: October 2022

Carbonic anhydrases (CAs) are ubiquitous enzymes that catalyze the reversible carbon dioxide hydration reaction. Among the eight different CA classes existing in nature, the α-class is the largest one being present in animals, bacteria, protozoa, fungi, and photosynthetic organisms. Although many studies have been reported on these enzymes, few functional, biochemical, and structural data are currently available on α-CAs isolated from photosynthetic organisms. Here, we give an overview of the most recent literature on the topic. In higher plants, these enzymes are engaged in both supplying CO2 at the Rubisco and determining proton concentration in PSII membranes, while in algae and cyanobacteria they are involved in carbon-concentrating mechanism (CCM), photosynthetic reactions and in detecting or signaling changes in the CO2 level in the environment. Crystal structures are only available for three algal α-CAs, thus not allowing to associate specific structural features to cellular localizations or physiological roles. Therefore, further studies on α-CAs from photosynthetic organisms are strongly needed to provide insights into their structure–function relationship.
Label-Free Enrichment of Circulating Tumor Plasma Cells: Future Potential Applications of Dielectrophoresis in Multiple Myeloma
Authors: Musso Nicolò , Romano Alessandra , Bonacci Paolo Giuseppe , Scandura Grazia , Pandino Clarissa , Camarda Massimo , Russo Giorgio Ivan , Di Raimondo Francesco , Cacciola Emma , Cacciola Rossella
Published: October 2022

In multiple myeloma (MM), circulating tumor plasma cells (CTPCs) are an emerging prognostic factor, offering a promising and minimally invasive means for longitudinal patient monitoring. Recent advances highlight the complex biology of plasma cell trafficking, highlighting the phenotypic and genetic signatures of intra- and extra-medullary MM onset, making CTPC enumeration and characterization a new frontier of precision medicine for MM patients, requiring novel technological platforms for their standardized and harmonized detection. Dielectrophoresis (DEP) is an emerging label-free cell manipulation technique to separate cancer cells from healthy cells in peripheral blood samples, based on phenotype and membrane capacitance that could be successfully tested to enumerate and isolate CTPCs. Herein, we summarize preclinical data on DEP development for CTPC detection, as well as their clinical and research potential.
HFR1, a bHLH Transcriptional Regulator from Arabidopsis thaliana, Improves Grain Yield, Shade and Osmotic Stress Tolerances in Common Wheat
Authors: Sun Guanghua , Yang Luhao , Zhan Weimin , Chen Shizhan , Song Meifang , Wang Lijian , Jiang Liangliang , Guo Lin , Wang Ke , Ye Xingguo , Gou Mingyue , Zheng Xu , Yang Jianping , Yan Zehong
Published: October 2022

Common wheat, Triticum aestivum, is the most widely grown staple crop worldwide. To catch up with the increasing global population and cope with the changing climate, it is valuable to breed wheat cultivars that are tolerant to abiotic or shade stresses for density farming. Arabidopsis LONG HYPOCOTYL IN FAR-RED 1 (AtHFR1), a photomorphogenesis-promoting factor, is involved in multiple light-related signaling pathways and inhibits seedling etiolation and shade avoidance. We report that overexpression of AtHFR1 in wheat inhibits etiolation phenotypes under various light and shade conditions, leading to shortened plant height and increased spike number relative to non-transgenic plants in the field. Ectopic expression of AtHFR1 in wheat increases the transcript levels of TaCAB and TaCHS as observed previously in Arabidopsis, indicating that the AtHFR1 transgene can activate the light signal transduction pathway in wheat. AtHFR1 transgenic seedlings significantly exhibit tolerance to osmotic stress during seed germination compared to non-transgenic wheat. The AtHFR1 transgene represses transcription of TaFT1, TaCO1, and TaCO2, delaying development of the shoot apex and heading in wheat. Furthermore, the AtHFR1 transgene in wheat inhibits transcript levels of PHYTOCHROME-INTERACTING FACTOR 3-LIKEs (TaPIL13, TaPIL15-1B, and TaPIL15-1D), downregulating the target gene STAYGREEN (TaSGR), and thus delaying dark-induced leaf senescence. In the field, grain yields of three AtHFR1 transgenic lines were 18.2–48.1% higher than those of non-transgenic wheat. In summary, genetic modification of light signaling pathways using a photomorphogenesis-promoting factor has positive effects on grain yield due to changes in plant architecture and resource allocation and enhances tolerances to osmotic stress and shade avoidance response.
Uncovering Novel Features of the Pc Locus in Horn Development from Gene-Edited Holstein Cattle by RNA-Sequencing Analysis
Authors: Wang Huan , Zhu Huabin , Hu Zhihui , Heng Nuo , Gong Jianfei , Wang Yi , Zou Huiying , Zhao Shanjiang
Published: October 2022

The Polled Celtic (Pc) mutation locus is a genetically simple single mutation that is the best choice for breeding polled cattle using gene editing. However, the mechanism of the Pc locus for regulating horn development is unclear, so we used gene editing, somatic cell nuclear transfer and embryo transfer to obtain polled Holstein fetal bovine (gestation time 90 days) with a homozygous Pc insertion (gene-edited Holstein fetal bovine, EH) and the wild-type 90 days Holstein fetal bovine (WH) as controls. The hematoxylin-eosin (HE) staining results showed that, compared to the WH, the EH horn buds had no white keratinized projections or vacuolated keratinocytes and no thick nerve bundles under the dermal tissue. Furthermore, DNA sequencing results showed that the Pc locus was homozygously inserted into the fetal bovine genome. A total of 791 differentially expressed genes were identified by transcriptome sequencing analysis. Enrichment analysis and protein interaction analysis results of differentially expressed genes showed that abundant gene changes after Pc insertion were associated with the adhesion molecule regulation, actin expression, cytoskeletal deformation and keratin expression and keratinization. It was also noted that the results contained several genes that had been reported to be associated with the development of horn traits, such as RXFP2 and TWIST1. This study identified these changes for the first time and summarized them. The results suggested that the Pc mutant locus may inhibit neural crest cell EMT generation and keratin expression, leading to failures in neural crest cell migration and keratinization of the horn bud tissue, regulating the production of the polled phenotype.
FIRE: A Finely Integrated Risk Evaluation Methodology for Life-Critical Embedded Systems
Authors: Rao Aakarsh , Carreón Nadir A. , Lysecky Roman , Rozenblit Jerzy
Published: October 2022

Life-critical embedded systems, including medical devices, are becoming increasingly interconnected and interoperable, providing great efficiency to the healthcare ecosystem. These systems incorporate complex software that plays a significantly integrative and critical role. However, this complexity substantially increases the potential for cybersecurity threats, which directly impact patients’ safety and privacy. With software continuing to play a fundamental role in life-critical embedded systems, maintaining its trustworthiness by incorporating fail-safe modes via a multimodal design is essential. Comprehensive and proactive evaluation and management of cybersecurity risks are essential from the very design to deployment and long-term management. In this paper, we present FIRE, a finely integrated risk evaluation methodology for life-critical embedded systems. Security risks are carefully evaluated in a bottom-up approach from operations-to-system modes by adopting and expanding well-established vulnerability scoring schemes for life-critical systems, considering the impact to patient health and data sensitivity. FIRE combines a static risk evaluation with runtime dynamic risk evaluation to establish comprehensive risk management throughout the lifecycle of the life-critical embedded system. We demonstrate the details and effectiveness of our methodology in systematically evaluating risks and conditions for risk mitigation with a smart connected insulin pump case study. Under normal conditions and eight different malware threats, the experimental results demonstrate effective threat mitigation by mode switching with a 0% false-positive mode switching rate.
Bio-Inspired Synthesis of Carbon-Based Nanomaterials and Their Potential Environmental Applications: A State-of-the-Art Review
Authors: Dutta Vishal , Verma Ritesh , Gopalkrishnan C. , Yuan Min-Hao , Batoo Khalid Mujasam , Jayavel R. , Chauhan Ankush , Lin Kun-Yi Andrew , Balasubramani Ravindran , Ghotekar Suresh
Published: October 2022

Providing safe drinking water and clean water is becoming a more challenging task all around the world. Although some critical issues and limits remain unsolved, implementing ecologically sustainable nanomaterials (NMs) with unique features, e.g., highly efficient and selective, earth-abundance, renewability, low-cost manufacturing procedures, and stability, has become a priority. Carbon nanoparticles (NPs) offer tremendous promise in the sectors of energy and the environment. However, a series of far more ecologically friendly synthesis techniques based on natural, renewable, and less expensive waste resources must be explored. This will reduce greenhouse gas emissions and harmful material extraction and assist the development of green technologies. The progress achieved in the previous 10 years in the fabrication of novel carbon-based NMs utilizing waste materials as well as natural precursors is reviewed in this article. Research on carbon-based NPs and their production using naturally occurring precursors and waste materials focuses on this review research. Water treatment and purification using carbon NMs, notably for industrial and pharmaceutical wastes, has shown significant potential. Research in this area focuses on enhanced carbonaceous NMs, methods, and novel nano-sorbents for wastewater, drinking water, groundwater treatment, as well as ionic metal removal from aqueous environments. Discussed are the latest developments and challenges in environmentally friendly carbon and graphene quantum dot NMs.
Mitogenomic Comparison of the Mole Crickets Gryllotalpidae with the Phylogenetic Implications (Orthoptera: Ensifera)
Authors: Ma Yan , Miao Ying
Published: October 2022

Owing to limited molecular data, the phylogenetic position of the family Gryllotalpidae is still controversial in the infraorder Gryllidea. Mitochondrial genome (mitogenome) plays a crucial role in reconstructing phylogenetic relationships and revealing the molecular evolution of insects. However, only four mitogenomes have been reported in Gryllotalpidae to date. Herein, we obtained the first mitogenomes of Gryllotalpa henana Cai & Niu, 1998 and the Chinese G. orientalis Burmeister, 1838, made a detailed comparison of all mitogenomes available in Gryllotalpidae and reconstructed the phylogeny of Gryllidea based on mitogenomes using Bayesian inference (BI) and maximum likelihood (ML) methods. The results show that the complete mitogenome sequences of G. henana (15,504 bp) and G. orientalis (15,497 bp) are conserved, both exhibiting the double-stranded circular structure, typical gene content and the ancestral insect gene arrangement. The complete mitogenome of G.henana exhibits the lowest average AT content ever detected in Gryllotalpidae, and even Gryllidea. The gene nad2 of both species has atypical initiation codon GTG. All tRNAs exhibit typical clover-leaf structure, except for trnS1 lacking the dihydrouridine (DHU) arm. A potential stem–loop structure, containing a (T)n(TC)2(T)n sequence, is detected in the control region of all gryllotalpids investigated and is likely related to the replication initiation of the minority strand. The phylogenetic analyses recover the six families of Gryllidea as Gryllotalpidae + (Myrmecophilidae + (Mogoplistidae + (Trigonidiidae + (Phalangopsidae + Gryllidae)))), similar to the trees based on transcriptomic and mitogenomic data. However, the trees are slightly different from the multilocus phylogenies, which show the sister-group relationship of Gryllotalpidae and Myrmecophilidae. The contradictions between mitogenomic and multilocus trees are briefly discussed.
IDeS Method Applied to an Innovative Motorbike—Applying Topology Optimization and Augmented Reality
Authors: Frizziero Leonardo , Leon-Cardenas Christian , Galiè Giulio , Alessandri Giulia , Iannarelli Lorenzo , Lucci Lisa , Merighi Sarah , Polignano Pietro
Published: October 2022

This study is on the conception of the DS700 HYBRID project by the application of the Industrial Design Structure method (IDeS), which applies different tools sourced from engineering and style departments, including QFD and SDE, used to create the concept of a hybrid motorbike that could reach the market in the near future. SDE is an engineering approach for the design and development of industrial design projects, and it finds important applications in the automotive sector. In addition, analysis tools such as QFD, comprising benchmarking and top-flop analysis are carried out to maximize the creative process. The key characteristics of the bike and the degree of innovation are identified and outlined, the market segment is identified, and the stylistic trends that are most suitable for a naked motorbike of the future are analyzed. In the second part the styling of each superstructure and of all the components of the vehicle is carried out. Afterwards the aesthetics and engineering perspectives are accounted for to complete the project. This is achieved with modelling and computing tools such as 3D CAD, visual renderings, and FEM simulations, and virtual prototyping thanks to augmented reality (AR), and finally physical prototyping with the use of additive manufacturing (AM). The result is a product conception able to compete in the present challenging market, with a design that is technically feasible and also reaches new lightness targets for efficiency.
Association between Serum Uric Acid and Hypertension in a Large Cross-Section Study in a Chinese Population
Authors: He Yang , Chen Du , Xu Jing-Ping , Jin Jun , Wang Jun , Geng Cong , He Yong-Ming
Published: October 2022

Background: The association of serum uric acid (SUA) with hypertension has been well established in Caucasian populations. However, its association with hypertension in Chinese remained to be clarified. Methods: Consecutive patients, homogeneous in Chinese Han ethnicity, aged ≥18 years, abstracted from the database, admitted from 1 January 2010 to 31 December 2013, were included for potential analysis. The patients were grouped according to the presence or absence of hypertension. Unconditional logistic regression was performed to estimate the association between SUA and hypertension. Its possible interactions with risk factors on hypertension were also explored. Results: A total of 9587 patients were finally analyzed in the current study, where 5692 were with hypertension and 3895 were without hypertension. Per 100 μmol/L higher SUA concentration was associated with multivariable-adjusted odds ratios (95% CI) of 1.25 (1.08–1.22) in males, 1.10 (1.01–1.20) in females, and 1.19 (1.13–1.24) in total. On a categorical scale, when compared with the first quintile, the multivariable-adjusted odds ratios (95% CI) were 1.40 (1.20–1.64) for the 2nd quintile, 1.48 (1.27–1.74) for the 3rd quintile, 1.55 (1.32–1.82) for the 4th quintile, and 1.92 (1.63–2.26) for the 5th quintile, with a p for trend < 0.01. Conclusions: SUA is associated with hypertension in a dose-response manner among the Chinese hospitalized population. Management of SUA could help to the prevention and control of hypertension.
Computed Tomography Imaging in ILD: New Trends for the Clinician
Authors: Zimmermann Gregor S.
Published: October 2022

Today, radiological methods are an integral part of diagnostics in lung diseases, and they provide important information regarding the evaluation of interstitial lung diseases (ILDs) [...]
Endovascular Treatment for Aneurysms Located in the Posterior Communicating Artery (PCoA) by the Swinging-Tail Technique: A Technical Note
Authors: Wang Jiejun , Zhang Longhui , Dong Linggen , Zhang Shuai , Zhu Haoyu , Jiang Chuhan , Lv Ming
Published: October 2022

Purposes: A stent-assisted coil (SAC) is a safe and effective treatment modality for some complex intracranial aneurysms, especially for wide neck aneurysms. However, some wide neck aneurysms with a tough angle and located in the posterior communicating artery (PCoA) are challenging to treat with a SAC. This study aimed to examine and discuss the swinging-tail technique for treating wide neck aneurysms located in the PCoA using a SAC by Prof. Lv. Materials and Methods: We retrospectively reviewed our institutional clinical database and identified nine patients with neck aneurysms located in the PCoA, and these patients underwent the swinging-tail technique by Prof. Lv, which is a novel technique of releasing a stent, from June 2016 to September 2021. Results: In this study, nine patients underwent SAC treatment using the swinging-tail technique by Prof. Lv. Aneurysmal complete occlusion was observed in every patient without any complications, as shown by immediate postoperative angiography. Additionally, the modified Rankin scale was monitored for clinical outcomes in the follow-up. One patient died postoperatively due to severe SAH with an intraventricular hemorrhage. Four of nine patients underwent imaging follow-up, demonstrating the complete occlusion of aneurysms; eight patients underwent clinical follow-up and achieved a favorable clinical outcome (modified Rankin scale score: 0–2). Conclusion: The SAC treatment for wide neck aneurysms located in the PCoA can be challenging for operators because of the specific location, resulting in inadequate vessel wall apposition by antegrade stenting via the ipsilateral vessel. In this circumstance, the swinging-tail technique may be a feasible and effective choice.
Association between SGLT2 Inhibitors and Cardiac Rehabilitation Outcomes in Patients with Cardiovascular Disease and Type 2 Diabetes Mellitus
Authors: Kashima Ayuko , Kamiya Kentaro , Hamazaki Nobuaki , Ueno Kensuke , Nozaki Kohei , Ichikawa Takafumi , Yamashita Masashi , Uchida Shota , Noda Takumi , Hotta Kazuki , Maekawa Emi , Yamaoka-Tojo Minako , Matsunaga Atsuhiko , Ako Junya
Published: October 2022

The use of sodium-glucose cotransporter 2 (SGLT2) inhibitors in patients with type 2 diabetes mellitus (T2DM) has been associated with decreased skeletal muscle mass but remains unclear in patients with cardiovascular disease (CVD) undergoing comprehensive outpatient cardiac rehabilitation (CR). Therefore, this study investigates the effect of SGLT2 inhibitors on the outcomes of patients with CVD and T2DM undergoing comprehensive outpatient CR. The study included 402 patients with CVD and T2DM who participated in comprehensive outpatient CR. Physical functions (grip strength, maximal quadriceps isometric strength, usual gait speed, and 6-minute walking distance) were measured at discharge as baseline and 5 months thereafter, and the association between physical functions and SGLT2 inhibitor use was reviewed. Physical functions improved regardless of SGLT2 inhibitor use. Multiple regression analysis showed that SGLT2 inhibitor use was not associated with improvement or decline in physical functions (p ≥ 0.05). The use of SGLT2 inhibitors in patients with CVD and T2DM undergoing outpatient CR did not impair improvement in physical functions.
Bronchiectasis in Adults: Aetiology and New Therapies
Authors: King Paul T. , Morgan Lucy
Published: October 2022

Bronchiectasis is emerging as a global health issue, and this is reflected by a series of registries that were established worldwide [...]
Imaging of GBM in the Age of Molecular Markers and MRI Guided Adaptive Radiation Therapy
Authors: Dajani Salah , Hill Virginia B. , Kalapurakal John A. , Horbinski Craig M. , Nesbit Eric G. , Sachdev Sean , Yalamanchili Amulya , Thomas Tarita O.
Published: October 2022

Glioblastoma (GBM) continues to be one of the most lethal malignancies and is almost always fatal. In this review article, the role of radiation therapy, systemic therapy, as well as the molecular basis of classifying GBM is described. Technological advances in the treatment of GBM are outlined as well as the diagnostic imaging characteristics of this tumor. In addition, factors that affect prognosis such as differentiating progression from treatment effect is discussed. The role of MRI guided radiation therapy and how this technology may provide a mechanism to improve the care of patients with this disease are described.
The UA Doppler Index, Plasma HCY, and Cys C in Pregnancies Complicated by Congenital Heart Disease of the Fetus
Authors: Xu Xiaona , Ye Baoying , Li Min , Xia Yuanqing , Wu Yi , Cheng Weiwei
Published: October 2022

Background: Congenital heart disease/defect (CHD) is one of the most common congenital disabilities. Early diagnosis of CHD can improve the prognosis of newborns with CHD. The aim of this study was to evaluate the relationship between the factors and the onset of fetal congenital heart disease by measuring fetal umbilical artery (UA) Doppler index, maternal HCY, and Cys C levels during pregnancy. Methods: This retrospective study analyzed 202 fetuses with CHD, including 77 cases (39.1%) of simple CHD and 120 cases (60.9%) of complex CHD. Singleton pregnant women who were examined at the same time and whose malformation screening did not suggest any structural abnormalities in the fetus were assigned to the control group (n = 400). The UA Doppler index, plasma HCY, and Cys C levels were compared among the pregnant women across the three groups, and logistic regression analysis was performed on statistically significant markers. The ROC of UA S/D, PI, RI, HCY, and Cys C were plotted, and the area under the ROC (AUC) was calculated. Results: The UA S/D, PI, and RI in the complex CHD group were significantly higher than those in the control group (p < 0.05). The levels of HCY and Cys C in the CHD group were significantly higher than those in the control group (p < 0.05). HCY and S/D revealed a positive correlation (r = 0.157), and the difference was statistically significant (p < 0.001). Cys C and S/D were positively correlated (r = 0.131), and the difference was statistically significant (p < 0.05). The levels of UA Doppler indices, maternal plasma HCY, and Cys C were elevated in fetuses with CHD. The AUC of the combined test of the UA index, HCY, and Cys C was higher than that of each individual test. Conclusions: Elevated levels of the UA doppler indices, HCY, and Cys C during pregnancy are positively associated with the development of congenital heart disease in offspring. The combination of HCY and Cys C was the most efficient test for the diagnosis of CHD. We are the first to report that plasma Cys C levels of women pregnant with fetuses with CHD were higher than those of women pregnant with normal fetuses.
Slovak Translation and Cross-Cultural Validation of the Eating Assessment Tool (EAT10®)
Authors: Frajkova Zofia , Paouris Dimitrios , Nado Ladislav , Vyrvova Ivana , Fabianova Adelaida , Printza Athanasia , Varga Lukas , Tedla Miroslav
Published: October 2022

Introduction: The objective of the study was the language adaptation and verification of psychometric properties of the Slovak version of the EAT10® questionnaire. Methods: The original English version of the questionnaire was translated into the Slovak language. The research group included 136 control participants and 51 dysphagic patients. Test–retest reliability, item analysis, internal consistency, construct and clinical validity, and Receiver Operating Characteristic (ROC) analysis were performed to verify the psychometric properties of the Slovak EAT10®. Results: The internal consistency assessed with Cronbach’s alpha is excellent (α = 0.94). Statistical analysis of the Slovak version of EAT10® showed excellent reliability (0.91, p < 0.001) in the test–retest. Through item-to-total correlation, we found out that all items significantly correlated with the overall score in EAT10®. Factor analysis proved a high construct validity. The EAT10® questionnaire was able to reveal a latent variable: a swallowing disorder, which was affecting a group of patients. The clinical validity results confirmed statistically significant differences in the mean scores of the control and dysphagic groups (z = −10.30; p < 0.001). By dividing the dysphagic group into four subgroups (Head and Neck Cancer, Extraesophageal Reflux, Iatrogenic, and Neurological) there were nonsignificant differences in the mean scores of the subgroups. The cut-off value for the Slovak EAT10® is three points. Conclusion: The Slovak EAT10® is a valid and reliable tool designed for the subjective assessment of oropharyngeal dysphagia in patients.