bluuebunny/crossref_metadata_2025 huggingface dataset:
Eco-Friendly OSN Membranes Based on Alginate Salts with Variable Nanofiltration Properties
Authors: Dmitrieva Evgenia , Raeva Alisa , Razlataya Daria , Anokhina Tatyana
Published: February 2023

In this work, membranes for organic solvents nanofiltration (OSN) based on a natural polymer, sodium alginate, were fabricated. They are chemically stable in organic solvents, including aprotic polar solvents. The unique advantage of these membranes is the absence of toxic reagents and solvents during their production. This ensures the safety and environmental friendliness of the production process. It has been shown that an operation as simple as changing the cation in alginate (Cu2+, Fe3+, Cr3+, Al3+, Zn2+, Ca2+) makes it possible to control the transport and separating properties of membranes, depending on the organic solvent being separated. Therefore, to isolate RemazolBrilliant Blue with MM = 626 g·mol−1 from ethanol, membranes based on iron alginate with a rejection R = 97% and a permeability of 1.5 kg·m−2·h−1·bar−1 are the most efficient. For isolation of the same solute from DMF and MP, membranes based on calcium alginate with an R of about 90% and a permeability of 0.1–0.2 kg·m−2·h−1·bar−1 are the most efficient. The resulting membranes based on natural biodegradable sodium alginate are competitive compared to membranes based on synthetic polymers.
Reactions and Morphologies of Mg and Mg/Teflon/Viton Particles during Oxidation
Authors: Li Yifan , Wang Jie , Shen Dong , Liu Haoying , Song Dongming , Li Yanchun
Published: February 2023

A thorough investigation on the reactions and morphologies of Mg particles and Mg/Teflon/Viton (MTV) during oxidation were conducted via thermal gravity-differential scanning calorimetry (TG-DSC) and scanning electronic microscopy. The results showed that the oxidation of Mg is fast. It merely changed the metallic luster of Mg before 550 °C, and only a few particles changed to a white irregular shape at 600 °C. However, all of the Mg particles oxidized to porous irregular shaped MgO at 650 °C. Herein, the oxidation of Mg particles ended by its melting point, and the whole process is a solid–gas-phase reaction. On the other hand, when MTV reacted in air, the reaction could be divided into two stages: the fluorination of Mg and the oxidation of the exceeded Mg. In the first stage, a dense MgF2 shell was formed by the solid–solid fluorination. The dense MgF2 shell could impede the oxidation of Mg. As a result, the oxidation of Mg began after its melting. Furthermore, liquid Mg could vaporize at higher temperature, which could burst out from the MgF2 shell and react with oxygen. The MgF2 shell exhibited a dense feature, not only protecting the Mg particles from the heterogeneous oxidation at lower temperature, but also facilitates the homogeneous oxidation at higher temperature.
Hydrogen Embrittlement of Inconel 718 Manufactured by Laser Powder Bed Fusion Using Sustainable Feedstock: Effect of Heat Treatment and Microstructural Anisotropy
Authors: Mohandas Naveen Karuthodi , Giorgini Alex , Vanazzi Matteo , Riemslag Ton , Scott Sean Paul , Popovich Vera
Published: February 2023

This study investigated the in-situ gaseous (under 150 bar) hydrogen embrittlement behaviour of additively manufactured (AM) Inconel 718 produced from sustainable feedstock. Here, sustainable feedstock refers to the Inconel 718 powder produced by vacuum induction melting inert gas atomisation of failed printed parts or waste from CNC machining. All Inconel 718 samples, namely AM-as-processed, AM-heat-treated and conventional samples showed severe hydrogen embrittlement. Additionally, it was found that despite its higher yield strength (1462 ± 8 MPa) and the presence of δ phase, heat-treated AM Inconel 718 demonstrates 64% lower degree of hydrogen embrittlement compared to the wrought counterpart (Y.S. 1069 ± 4 MPa). This was linked to the anisotropic microstructure induced by the AM process, which was found to cause directional embrittlement unlike the wrought samples showing isotropic embrittlement. In conclusion, this study shows that AM Inconel 718 produced from recycled feedstock shows better hydrogen embrittlement resistance compared to the wrought sample. Furthermore, the unique anisotropic properties, seen in this study for Inconel 718 manufactured by laser powder bed fusion, could be considered further in component design to help minimise the degree of hydrogen embrittlement.
Current Status and Challenges of Powder Bed Fusion-Based Metal Additive Manufacturing: Literature Review
Authors: Dejene Naol Dessalegn , Lemu Hirpa G.
Published: February 2023

Powder bed fusion (PBF) is recognized as one of the most common additive manufacturing technologies because of its attractive capability of fabricating complex geometries using many possible materials. However, the quality and reliability of parts produced by this technology are observed to be crucial aspects. In addition, the challenges of PBF-produced parts are hot issues among stakeholders because parts are still insufficient to meet the strict requirements of high-tech industries. This paper discusses the present state of the art in PBF and technological challenges, with a focus on selective laser melting (SLM). The review work focuses mainly on articles that emphasize the status and challenges of PBF metal-based AM, and the study is primarily limited to open-access sources, with special attention given to the process parameters and flaws as a determining factor for printed part quality and reliability. Moreover, the common defects due to an unstrained process parameter of SLM and those needed to monitor and sustain the quality and reliability of components are encompassed. From this review work, it has been observed that there are several factors, such as laser parameters, powder characteristics, material properties of powder and the printing chamber environments, that affect the SLM printing process and the mechanical properties of printed parts. It is also concluded that the SLM process is not only expensive and slow compared with conventional manufacturing processes, but it also suffers from key drawbacks, such as its reliability and quality in terms of dimensional accuracy, mechanical strength and surface roughness.
Evaluation of Mechanical Properties of Different Casing Drilling Steels
Authors: He Xiaoyu , Zhang Min , Xu Tianhan , Lei Longyu , Li Yi
Published: February 2023

An investigation into the mechanical properties of K55, N80, and P110 steels commonly used for casing drilling was carried out together with microstructural and fractographic analysis. The results show that P110 steel consisted of almost fully tempered martensite and exhibited a synergy combination of static tensile, dynamic impact, and fatigue crack propagation properties among the three steels, possessing a higher fatigue limit and deeper crack tolerance before failure occurred. The K55 steel consisted of the pearlite and network structure of ferrite and possessed a high strain hardening exponent and low impact property, which led to the more suitable application under incidental large overload and temperature change, but it was unsuitable under the condition of higher impact force. The properties of N80 steel were moderate, and its fatigue property was higher than that of K55 and lower than that of P110; its incidental overload resistance was also higher than that of P110. The casing drilling steel can be selected according to the environment.
Tribo-Mechanical Behavior of Films and Modified Layers Produced by Cathodic Cage and Glow Discharge Plasma Nitriding Techniques
Authors: Schibicheski Kurelo Bruna C. E. , De Souza Gelson Biscaia , Da Silva Silvio Luiz Rutz , Lepienski Carlos Maurício , Alves Júnior Clodomiro , Chuproski Rafael Fillus , Pintaúde Giuseppe
Published: February 2023

Two surface modification techniques, the glow discharge plasma nitriding (GDPN) and the cathodic cage plasma nitriding (CCPN), were compared regarding the mechanical and tribological behavior of layers produced on AISI 316 stainless-steel surfaces. The analyses were carried out at the micro/nanoscale using nanoindentation and nanoscratch tests. The nitriding temperature (°C) and time (h) parameters were 350/6, 400/6, and 450/6. Morphology, structure, and microstructure were evaluated by X-ray diffraction, scanning electron and optical interferometry microscopies, and energy-dispersive X-ray spectroscopy. GDPN results in stratified modified surfaces, solidly integrated with the substrate, with a temperature-dependent composition comprising nitrides (γ’-Fe4N, ε-Fe2+xN, CrN) and N-solid solution (γN phase). The latter prevails for the low treatment temperatures. Hardness increases from ~2.5 GPa (bare surface) to ~15.5 GPa (450 °C). The scratch resistance of the GDPN-modified surfaces presents a strong correlation with the layer composition and thickness, with the result that the 400 °C condition exhibits the highest standards against microwear. In contrast, CCPN results in well-defined dual-layers for any of the temperatures. A top 0.3–0.8 µm-thick nitride film (most ε-phase), brittle and easily removable under scratch with loads as low as 63 mN, covers a γN-rich case with hardness of 10 GPa. The thickness of the underneath CCPN layer produced at 450 °C is similar to that from GDPN at 400 °C (3 µm); on the other hand, the average roughness is much lower, comparable to the reference surface (Ra ~10 nm), while the layer formation involves no chromium depletion. Moreover, edge effects are absent across the entire sample´s surface. In conclusion, among the studied conditions, the GDPN 400 °C disclosed the best tribo-mechanical performance, whereas CCPN resulted in superior surface finishing for application purposes.
Flexural Behavior of Stainless Steel V-Stiffened Lipped Channel Beams
Authors: Huangfu Shuang-E , Tao Zhong , Zhang Ji , Abdul Ghafar Wahab , Wang Zihao , Ye Caifeng , Hasan Md Mehedi
Published: February 2023

Six groups of austenitic 022Cr19Ni10 stainless steel bending specimens with three types of cross-sectional forms were used to study the impact of V-stiffeners on the failure mode and flexural behavior of stainless steel lipped channel beams. These cross-sectional forms included V-stiffeners in the web compression zone at 1/3 height near the compressed flange and no V-stiffeners on the flange, V-stiffeners in the web compression zone at 1/3 height near the compressed flange and V-stiffeners in the middle of the compressed flange, and V-stiffeners on the web at 1/2 height and V-stiffeners in the middle of the compressed and tensioned flange. The findings show that the specimens without a V-stiffener on the flange and with a V-stiffener in the web compression zone at 1/3 height near the compressed flange have a lower ultimate bearing capacity as a result of local–distortional interaction buckling. The test specimen’s flexural bearing capacity will rise with an increase in web height under the same stiffening form and other fixed cross-sectional parameters. Furthermore, the additional V-stiffeners in the middle of the flange effectively lower the width-to-thickness ratio of the stainless steel specimen with a flange that has been V-stiffened, thus increasing the specimen’s bending bearing capacity by 13% and emphasizing the distortional buckling issue. Buckling first manifests in the compression flange and is caused by the stress evolution on the web of the bending specimen, which is constrained by the V-stiffeners. The specimen’s capacity to withstand bending loads can be improved more successfully through the use of V-stiffeners in the web compression zone at 1/3 height close to the compressed flange and V-stiffeners in the middle of the compressed flange. For the test data, a finite element simulation was established, and the results are generally consistent with the test results.
Machine Learning-Based Integration of Metabolomics Characterisation Predicts Progression of Myopic Retinopathy in Children and Adolescents
Authors: Hou Xiao-Wen , Yang Jin-Liu-Xing , Li Dan-Lin , Tao Yi-Jin , Ke Chao-Fu , Zhang Bo , Liu Shang , Cheng Tian-Yu , Wang Tian-Xiao , Xu Xun , He Xian-Gui , Pan Chen-Wei
Published: February 2023

Myopic retinopathy is an important cause of irreversible vision loss and blindness. As metabolomics has recently been successfully applied in myopia research, this study sought to characterize the serum metabolic profile of myopic retinopathy in children and adolescents (4–18 years) and to develop a diagnostic model that combines clinical and metabolic features. We selected clinical and serum metabolic data from children and adolescents at different time points as the training set (n = 516) and the validation set (n = 60). All participants underwent an ophthalmologic examination. Untargeted metabolomics analysis of serum was performed. Three machine learning (ML) models were trained by combining metabolic features and conventional clinical factors that were screened for significance in discrimination. The better-performing model was validated in an independent point-in-time cohort and risk nomograms were developed. Retinopathy was present in 34.2% of participants (n = 185) in the training set, including 109 (28.61%) with mild to moderate myopia. A total of 27 metabolites showed significant variation between groups. After combining Lasso and random forest (RF), 12 modelled metabolites (mainly those involved in energy metabolism) were screened. Both the logistic regression and extreme Gradient Boosting (XGBoost) algorithms showed good discriminatory ability. In the time-validation cohort, logistic regression (AUC 0.842, 95% CI 0.724–0.96) and XGBoost (AUC 0.897, 95% CI 0.807–0.986) also showed good prediction accuracy and had well-fitted calibration curves. Three clinical characteristic coefficients remained significant in the multivariate joint model (p < 0.05), as did 8/12 metabolic characteristic coefficients. Myopic retinopathy may have abnormal energy metabolism. Machine learning models based on metabolic profiles and clinical data demonstrate good predictive performance and facilitate the development of individual interventions for myopia in children and adolescents.
Metabolic Deregulations in Patients with Polycystic Ovary Syndrome
Authors: Jabczyk Marzena , Nowak Justyna , Jagielski Paweł , Hudzik Bartosz , Kulik-Kupka Karolina , Włodarczyk Aleksander , Lar Katarzyna , Zubelewicz-Szkodzińska Barbara
Published: February 2023

Polycystic ovary syndrome (PCOS) contributes to endocrine and metabolic complications for women worldwide. The aim of this study was to establish the usefulness of new anthropometric indices and atherogenic indices in the evaluation of metabolic disorders, in particular, glucose and insulin abnormalities in the profiles of women with polycystic ovary syndrome (PCOS). In the study, a total of 49 women with PCOS aged between 18 and 39 years were recruited. All patients were tested for fasting glucose and insulin, lipid parameters, oral-glucose administration, and biochemical parameters. All of them underwent anthropometric measurements, such as BMI (body mass index), WHR (waist-to-hip ratio), WHtR (waist-to-height ratio), BAI (body adiposity index), VAI (visceral adiposity index), LAP (lipid accumulation product), BRI (body roundness index), ABSI (A body shape index), AIP (atherogenic risk of plasma), AC (atherogenic coefficient), Castelli risk index-I, Castelli risk index-II and (LCI) lipoprotein combine index, TG/HDL-C ratio, METS-IR (The metabolic score of insulin resistance), triglyceride glucose index (TyG index), triglyceride glucose-body mass index (TyG-BMI index) and triglyceride glucose-waist circumference index (TyG-WC index) were calculated. The analyzed anthropometric measurements/indices and atherogenic indices demonstrated significant correlations in PCOS women. T A strong relationship was found between fasting glucose, fasting insulin, glucose after 60 min, HOMA-IR index in the patients with PCOS. There was no significant relationship between HbA1c and other analyzed parameters and indices. Most of the analyzed anthropometric and atherogenic indices may be useful tools in evaluating metabolic disorders, and, in particular, glucose and insulin abnormalities in PCOS women.
Structural Colors on Al Surface via Capped Cu-Si3N4 Bilayer Structure
Authors: Rahman M. A. , Kim Dongkyu , Arora Deepshikha , Huh Joo-Youl , Byun Ji Young
Published: February 2023

Tunable structural colors have a multitude of applications in the beautification of mobile devices, in the decoration of artwork, and in the creation of color filters. In this paper, we describe a Metal-Insulator-Metal (MIM) design that can be used to systematically tune structural colors by altering the thickness of the top metal and intermediate insulator. Cu and Si3N4 were selected as the top metal and intermediate insulator layers, respectively, and various reflection colors were printed on Al. To protect the Cu surface from scratchiness and oxidation, a number of capping layers, including SiO2, LPSQ, PMMA, and the commercially available clear coat ProtectaClear, were applied. In addition to their ability to protect Cu from a humid environment without deteriorating color quality, ProtectaClear and LPSQ coatings have minimal angle dependency. Furthermore, a bilayer of PMMA/SiO2 can protect the Cu surface from the effects of humidity. In addition, the PMMA/SiO2 and ProtectaClear/SiO2 bilayers can also protect against corrosion on the Cu surface. The colors can be tuned by controlling the thickness of either the metal layer or intermediate insulator layer, and vivid structural colors including brown, dark orange, blue, violet, magenta, cyan, green-yellow, and yellow colors can be printed. The measured dielectric functions of Cu thin films do not provide any evidence of the plasmonic effect, and therefore, it is expected that the obtained colors are attributed to thin-film interference.
Conduction Conditions for Self-Healing of Metal Interconnect Using Copper Microparticles Dispersed with Silicone Oil
Authors: Suetsugu Naoki , Iwase Eiji
Published: February 2023

This study clarifies the conditions for the bridging and conduction of a gap on a metal interconnect using copper microparticles dispersed with silicon oil. An AC voltage applied to a metal interconnect with a gap covered by a dispersion of metal microparticles traps the metal microparticles in the gap owing to the influence of a dielectrophoretic force on the interconnect, thus forming a metal microparticle chain. The current was tuned independently of the applied voltage by changing the external resistance. An AC voltage of 32 kHz was applied to a 10 µm wide gap on a metal interconnect covered with 3 µm diameter copper microparticles dispersed with silicone oil. Consequently, the copper microparticle chains physically bridged the interconnect and exhibited electrical conductivity at an applied voltage of 14 Vrms or higher and a post-bridging current of 350 mArms or lower. It was shown that the copper microparticle chains did not exhibit electrical conductivity at low applied voltages, even if the microparticle chains bridged the gap. A voltage higher than a certain value was required to achieve electrical conductivity, whereas an excessive voltage caused bubble formation and destroyed the bridges. These phenomena were explained based on the applied voltage and reference value of the current after bridging.
Investigation and Research of High-Performance RF MEMS Switches for Use in the 5G RF Front-End Modules
Authors: Tkachenko Alexey , Lysenko Igor , Kovalev Andrey
Published: February 2023

In this article, based on the developed methodology, the stages of designing two designs of high-performance radio-frequency single-pole single-throw microelectromechanical switches are investigated. These radio-frequency microelectromechanical switches are designed to operate at a central resonant frequency of 3.6 GHz and 3.4 GHz, respectively, as well as to work both in mobile communication devices and in the design of the architecture of 5G mobile networks, in particular in arrays of integrated antennas and radio-frequency interface modules. The manufacture and study of two designed structures are researched. For the first manufactured experimental sample in the open state the insertion loss is no more than −0.69 dB and the reflection loss is −28.35 dB, and in the closed state the isolation value is at least −54.77 dB at a central resonant frequency of 3.6 GHz. For the second in the open state the value of the insertion loss is no more than −0.67 dB and the reflection loss is −20.7 dB, and in the closed state the isolation value is not less than −52.13 dB at the central resonant frequency of 3.4 GHz. Both manufactured experimental samples are characterized by high linearity, as well as a small value of contact resistance in the closed state.
A Model-Driven Platform for Dynamic Partially Reconfigurable Architectures: A Case Study of a Watermarking System
Authors: Dalbouchi Roukaya , Trabelsi Chiraz , Elhajji Majdi , Zitouni Abdelkrim
Published: February 2023

The reconfigurable feature of FPGAs (Field-Programmable Gate Arrays) has made them a very attractive solution for implementing adaptive systems-on-chip. However, this implies additional design tasks to handle system reconfiguration and control, which increases design complexity. To address this issue, this paper proposes a model-driven design flow that guides the designer through the description of the different elements of a reconfigurable system. It is based on high-level modeling using an extended version of the MARTE (Modeling and Analysis of Real-Time and Embedded systems) UML (Unified Modeling Language) profile. Both centralized and decentralized reconfiguration decision-making solutions are possible with the proposed flow, allowing it to adapt to various reconfigurable systems constraints. It also integrates the IP-XACT standard (standard for the description of electronic Intellectual Properties), allowing the designer to easily target different technologies and commercial FPGAs by reusing both high-level models and actual IP-XACT hardware components. At the end of the flow, the implementation code is generated automatically from the high-level models. The proposed design flow was validated through a reconfigurable video watermarking application as a case study. Experimental results showed that the generated system allowed a good trade-off between resource usage, power consumption, execution time, and image quality compared to static implementations. This hardware efficiency was achieved in a very short time thanks to the design acceleration and automation offered by model-driven engineering.
SARS-CoV-2 Affects Both Humans and Animals: What Is the Potential Transmission Risk? A Literature Review
Authors: Santaniello Antonio , Perruolo Giuseppe , Cristiano Serena , Agognon Ayewa Lawoe , Cabaro Serena , Amato Alessia , Dipineto Ludovico , Borrelli Luca , Formisano Pietro , Fioretti Alessandro , Oriente Francesco
Published: February 2023

In March 2020, the World Health Organization Department declared the coronavirus (COVID-19) outbreak a global pandemic, as a consequence of its rapid spread on all continents. The COVID-19 pandemic has been not only a health emergency but also a serious general problem as fear of contagion and severe restrictions put economic and social activity on hold in many countries. Considering the close link between human and animal health, COVID-19 might infect wild and companion animals, and spawn dangerous viral mutants that could jump back and pose an ulterior threat to us. The purpose of this review is to provide an overview of the pandemic, with a particular focus on the clinical manifestations in humans and animals, the different diagnosis methods, the potential transmission risks, and their potential direct impact on the human–animal relationship.
Diversity of Bacterial Clones and Plasmids of NDM-1 Producing Escherichia coli Clinical Isolates in Central Greece
Authors: Tsilipounidaki Katerina , Florou Zoi , Skoulakis Anargyros , Fthenakis George C. , Miriagou Vivi , Petinaki Efthymia
Published: February 2023

The objective of the present study was to genetically characterize ten NDM-1 producing Escherichia coli isolates, recovered from patients in a hospital in Central Greece during the period 2017 to 2021.The isolates were studied by whole genome sequencing to obtain multi-locus sequencing typing (MLST), identification of blaNDM1-environment, resistome and plasmid content. MLST analysis showed the presence of eight sequence types: ST46* (two isolates), ST46, ST744, ST998, ST410, ST224, ST4380, ST683 and ST12 (one isolate each). Apart of the presence of blaNDM-1, the isolates carried a combination of various to β-lactams encoding resistance genes: blaTEM-1B, blaCTX-15, blaOXA-1, blaVIM-1, blaSHV-5, blaOXA-16, blaOXA-10 and blaVEB-1. Additionally, plurality of resistance genes to aminoglycosides, macrolides, rifamycin, phenicols, sulfonamides and tetracycline was detected. The presence of multiple replicons was observed, with predominance of IncFII and IncFIB. Analysis of blaNDM-1 genetic environment of the isolates showed that seven had 100% identity with the pS-3002cz plasmid (Accession Number KJ 958927), two with the pB-3002cz plasmid (Accession Number KJ958926) and one with the pEc19397-131 plasmid (Accession Number MG878866). Τhis latter plasmid was derived by the fusion of two, previously identified, plasmids, pAMPD2 and pLK75 (Accession Numbers CP078058 and KJ440076, respectively). The diversity of clones and plasmids of NDM-1 producing E. coli isolated from patients in Greece indicates a continuous horizontal gene transfer.
Risk of Exposure to Coccidioides spp. in the Temblor Special Recreation Management Area (SRMA), Kern County, CA
Authors: Lauer Antje , Lopez Jocelyne Jassiri , Chabolla Michael , Kloock Carl
Published: February 2023

The Temblor Mountain Special Recreation Area (SRMA) on the western flank of the San Joaquin Valley, CA, is located in the endemic area of Coccidioides, a fungal pathogen responsible for the increasing incidence of coccidioidomycosis (Valley fever). Recreationists in the SRMA, such as off-highway vehicle (OHV) drivers and mountain bikers who disturb the soils, are at risk of being exposed to airborne arthroconidia (asexual spores) of the pathogen. To reduce the risk of pathogen exposure for visitors, the Bureau of Land Management (BLM) plans to limit recreational activities to areas with a reduced pathogen presence. They envision an official OHV park in the future, by also restricting access to areas with ongoing restoration efforts and by limiting soil erosion in sensitive areas. To investigate which soils in the Temblor SRMA are most likely to support the growth of Coccidioides spp., soil samples were collected over a 3-year period from dominant soil types in a northern and a southern sampling area and analyzed for the pathogen using a culture-independent PCR-based method. In addition, soil pH and electrical conductivity were determined. The results of this study revealed slight genetic variance in the Coccidioides sequences obtained from the soils of the Temblor SRMA. An analysis of variance (ANOVA) could not confirm differences in soil pH and electrical conductivity (EC) between the different soil types investigated and between sites where the pathogen was detected compared to sites where it could not be found. However, the year of sampling appeared to have an influence on observed soil pH and EC, and the presence of the pathogen. Of all dominant soil types investigated, those belonging to the Littlesignal–Cochora association were the least likely to contain the pathogen, whereas soils of the Beam–Panoza–Hillbrick association appeared more supportive. In addition to pointing out OHV areas with lower pathogen exposure risk in the Temblor SRMA, recommendations were made to educate visitors and BLM workers about the risk of contracting Valley fever.
Assessing the Effects of Rotifer Feed Enrichments on Turbot (Scophthalmus maximus) Larvae and Post-Larvae Gut-Associated Bacterial Communities
Authors: Louvado Antonio , Castro Carolina , Silva Davide A. M. , Oliveira Vanessa , Conceição Luís E. C. , Cleary Daniel F. R. , Gomes Newton C. M.
Published: February 2023

Live feed enrichments are often used in fish larvicultures as an optimized source of essential nutrients to improve larval growth and survival. In addition to this, they may also play an important role in structuring larval-associated microbial communities and may help improve their resistance to diseases. However, there is limited information available on how larval microbial communities and larviculture water are influenced by different live feed enrichments. In the present study, we investigated the effects of two commercial rotifer enrichments (ER) on turbot (Scophthalmus maximus) larval and post-larval gut-associated bacterial communities during larviculture production. We evaluated their effects on bacterial populations related to known pathogens and beneficial bacteria and their potential influence on the composition of bacterioplankton communities during larval rearing. High-throughput 16S rRNA gene sequencing was used to assess the effects of different rotifer enrichments (ER1 and ER2) on the structural diversity of bacterial communities of the whole turbot larvae 10 days after hatching (DAH), the post-larval gut 30 DAH, and the larviculture water. Our results showed that different rotifer feed enrichments were associated with significant differences in bacterial composition of turbot larvae 10 DAH, but not with the composition of larval gut communities 30 DAH or bacterioplankton communities 10 and 30 DAH. However, a more in-depth taxonomic analysis showed that there were significant differences in the abundance of Vibrionales in both 10 DAH larvae and in the 30 DAH post-larval gut fed different RE diets. Interestingly, the ER1 diet had a higher relative abundance of specific amplicon sequence variants (ASVs) related to potential Vibrio-antagonists belonging to the Roseobacter clade (e.g., Phaeobacter and Ruegeria at 10 DAH and Sulfitobacter at 30 DAH). In line with this, the diet was also associated with a lower relative abundance of Vibrio and a lower mortality. These results suggest that rotifer diets can affect colonization by Vibrio members in the guts of post-larval turbot. Overall, this study indicates that live feed enrichments can have modulatory effects on fish bacterial communities during the early stages of development, which includes the relative abundances of pathogenic and antagonist taxa in larviculture systems.
Prevalence, Virulence Potential, and Growth in Cheese of Bacillus cereus Strains Isolated from Fresh and Short-Ripened Cheeses Sold on the Italian Market
Authors: Tirloni Erica , Bernardi Cristian , Celandroni Francesco , Mazzantini Diletta , Massimino Mariacristina , Stella Simone , Ghelardi Emilia
Published: February 2023

This study investigated B. cereus presence in 122 samples belonging to 34 typologies of fresh or short-ripened cheeses made from cow, sheep, goat, or buffalo pasteurized milk, and sold on the Italian market. B. cereus was isolated at a prevalence of 9.8%, with a marked variability among cheese categories, and at low counts (always below 2.26 Log CFU/g). Twelve isolates were identified by MALDI-TOF analysis and typified by RAPD PCR as belonging to different B. cereus strains. All the strains were tested for the production of hemolysin BL, phosphatidylcholine-specific phospholipase C, proteases, and biofilm formation, and for the presence of chromosomal toxin-encoding genes (sph, plcA, cytK, entFM, bcet, nheA, nheB, nheC). Overall, 92% of strains harbored bcet, 75% the three genes nheA, nheB, and nheC, as well as plcA and sph, 67% entFM, and 33% cytK. All strains showed biofilm-forming ability. A chemical-physical characterization of the cheeses was also performed to show their suitability as substrates for B. cereus growth, showing high heterogeneity in terms of pH, aw, salt content, and concentration of organic acids. Finally, the ability to support spore germination and vegetative cell growth of a selected cheese was investigated in spores-inoculated samples maintained at 10 °C and 15 °C, showing the inhibitory effect of low storage temperatures.
Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation
Authors: Funtikova Tatiana Vyacheslavovna , Akhmetov Lenar Imametdinovich , Puntus Irina Filippovna , Mikhailov Pavel Alexeevich , Appazov Nurbol Orynbasaruly , Narmanova Roza Abdibekovna , Filonov Andrey Evgenievich , Solyanikova Inna Petrovna
Published: February 2023

A new biopreparation is developed to clean soils from oil pollution in the arid climate of the Republic of Kazakhstan. The biopreparation includes bacterial strains R. qingshengii F2-1, R. qingshengii F2-2, and P. alloputida BS3701. When using the biopreparation in a liquid mineral medium with 15% crude oil, laboratory studies have revealed degradation of 48% n-alkanes and 39% of PAHs after 50 days. The effectiveness of the biopreparation has been demonstrated in field experiments in the soil contaminated with 10% crude oil at the K-Kurylys landfill, Republic of Kazakhstan. During the six-month field experiment, the number of oil degraders reached 107 CFU/g soil, which degraded 70% of crude oil by the end of the experiment.
Mobile Phones: Reservoirs of Resistant Bacteria during the COVID-19 Pandemic in Abu Dhabi, United Arab Emirates
Authors: Kayed Kawthar , Khoder Ghalia , Farhat Joviana , Ghemrawi Rose
Published: February 2023

Background: Mobile phones are excessively used even though microbes’ ability to survive on phone surfaces was confirmed. During the COVID-19 pandemic, heavy hygiene practices have been applied to mobile surfaces. Therefore, it is interesting to evaluate the emergence of antimicrobial-resistant bacteria on mobile phone surfaces. Methods: A random sampling technique was utilized on residents in Abu Dhabi, UAE between May and June 2021. A swab sample from each participant’s mobile phone was collected and transported to the microbiology laboratory for bacterial culture and antimicrobial susceptibility tests. Furthermore, a cross-sectional study was conducted via a self-administered questionnaire filled by participants. The questionnaire was used to collect sociodemographic data, phone frequency usage and cleaning methods. Results: One hundred two-sample swabs and data have been included in the study. The majority of participants (91.1%) reported cleaning their mobile phones with wipes and alcohol. However, 100% of participants had a mobile phone contaminated by bacteria such as S. aureus, E. coli, Coagulase-negative staphylococci, Micrococcus, Bacillus, Streptococcus, Citrobacter, Proteus, Enterococcus, klebsiella, Pseudomonas and Actinobacteria. Interestingly, most of these potentially pathogenic bacteria were found to be resistant to ampicillin, ceftazidime and cefotaxime. Conclusion: The continuous hand and mobile disinfectant have contributed to the emergence of resistant bacteria.
Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3
Authors: Asimakoula Stamatia , Marinakos Orfeas , Tsagogiannis Epameinondas , Koukkou Anna-Irini
Published: February 2023

Phenol poses a threat as one of the most important industrial environmental pollutants that must be removed before disposal. Biodegradation is a cost-effective and environmentally friendly approach for phenol removal. This work aimed at studying phenol degradation by Pseudarthrobacter phenanthrenivorans Sphe3 cells and also, investigating the pathway used by the bacterium for phenol catabolism. Moreover, alginate-immobilized Sphe3 cells were studied in terms of phenol degradation efficiency compared to free cells. Sphe3 was found to be capable of growing in the presence of phenol as the sole source of carbon and energy, at concentrations up to 1500 mg/L. According to qPCR findings, both pathways of ortho- and meta-cleavage of catechol are active, however, enzymatic assays and intermediate products identification support the predominance of the ortho-metabolic pathway for phenol degradation. Alginate-entrapped Sphe3 cells completely degraded 1000 mg/L phenol after 192 h, even though phenol catabolism proceeds slower in the first 24 h compared to free cells. Immobilized Sphe3 cells retain phenol-degrading capacity even after 30 days of storage and also can be reused for at least five cycles retaining more than 75% of the original phenol-catabolizing capacity.
Partial Substitution of Urea with Biochar Induced Improvements in Soil Enzymes Activity, Ammonia-Nitrite Oxidizers, and Nitrogen Uptake in the Double-Cropping Rice System
Authors: Ullah Saif , Ali Izhar , Yang Mei , Zhao Quan , Iqbal Anas , Wu Xiaoyan , Ahmad Shakeel , Muhammad Ihsan , Khan Abdullah , Adnan Muhammad , Yuan Pengli , Jiang Ligeng
Published: February 2023

Biochar is an important soil amendment that can enhance the biological properties of soil, as well as nitrogen (N) uptake and utilization in N-fertilized crops. However, few studies have characterized the effects of urea and biochar application on soil biochemical traits and its effect on paddy rice. Therefore, a field trial was conducted in the early and late seasons of 2020 in a randomized complete block design with two N levels (135 and 180 kg ha−1) and four levels of biochar (0, 10, 20, and 30 t ha−1). The treatment combinations were as follows: 135 kg N ha−1 + 0 t B ha−1 (T1), 135 kg N ha−1 + 10 t B ha−1 (T2), 135 kg N ha−1 + 20 t B ha−1 (T3), 135 kg N ha−1 + 30 t B ha−1 (T4), 180 kg N ha−1 + 0 t B ha−1 (T5), 180 kg N ha−1 + 10 t B ha−1 (T6), 180 kg N ha−1 + 20 t B ha−1 (T7) and 180 kg N ha−1 + 30 t B ha−1 (T8). The results showed that soil amended with biochar had higher soil pH, soil organic carbon content, total nitrogen content, and mineral nitrogen (NH4+-N and NO3−-N) than soil that had not been amended with biochar. In both seasons, the 20 t ha−1 and 30 t ha−1 biochar treatments had the highest an average concentrations of NO3–-N (10.54 mg kg−1 and 10.25 mg kg−1, respectively). In comparison to soil that had not been treated with biochar, the average activity of the enzymes urease, polyphenol oxidase, dehydrogenase, and chitinase was, respectively, 25.28%, 14.13%, 67.76%, and 22.26% greater; however, the activity of the enzyme catalase was 15.06% lower in both seasons. Application of biochar considerably increased the abundance of ammonia-oxidizing bacteria (AOB), which was 48% greater on average in biochar-amended soil than in unamended soil. However, there were no significant variations in the abundances of ammonia-oxidizing archaea (AOA) or nitrite-oxidizing bacteria (NOB) across treatments. In comparison to soil that had not been treated with biochar, the average N content was 24.46%, 20.47%, and 19.08% higher in the stem, leaves, and panicles, respectively. In general, adding biochar at a rate of 20 to 30 t ha−1 with low-dose urea (135 kg N ha−1) is a beneficial technique for improving the nutrient balance and biological processes of soil, as well as the N uptake and grain yield of rice plants.
Gut Microbiota as Well as Metabolomes of Wistar Rats Recover within Two Weeks after Doripenem Antibiotic Treatment
Authors: Murali Aishwarya , Zickgraf Franziska Maria , Ternes Philipp , Giri Varun , Cameron Hunter James , Sperber Saskia , Haake Volker , Driemert Peter , Kamp Hennicke , Weyer Dorothee Funk , Sturla Shana J. , Rietjens Ivonne M. G. M. , van Ravenzwaay Bennard
Published: February 2023

An understanding of the changes in gut microbiome composition and its associated metabolic functions is important to assess the potential implications thereof on host health. Thus, to elucidate the connection between the gut microbiome and the fecal and plasma metabolomes, two poorly bioavailable carbapenem antibiotics (doripenem and meropenem), were administered in a 28-day oral study to male and female Wistar rats. Additionally, the recovery of the gut microbiome and metabolomes in doripenem-exposed rats were studied one and two weeks after antibiotic treatment (i.e., doripenem-recovery groups). The 16S bacterial community analysis revealed an altered microbial population in all antibiotic treatments and a recovery of bacterial diversity in the doripenem-recovery groups. A similar pattern was observed in the fecal metabolomes of treated animals. In the recovery group, particularly after one week, an over-compensation was observed in fecal metabolites, as they were significantly changed in the opposite direction compared to previously changed metabolites upon 28 days of antibiotic exposure. Key plasma metabolites known to be diagnostic of antibiotic-induced microbial shifts, including indole derivatives, hippuric acid, and bile acids were also affected by the two carbapenems. Moreover, a unique increase in the levels of indole-3-acetic acid in plasma following meropenem treatment was observed. As was observed for the fecal metabolome, an overcompensation of plasma metabolites was observed in the recovery group. The data from this study provides insights into the connectivity of the microbiome and fecal and plasma metabolomes and demonstrates restoration post-antibiotic treatment not only for the microbiome but also for the metabolomes. The importance of overcompensation reactions for health needs further studies.
The Correlation of Colour and Iron Oxides in Yellow Seal Stones from Northern Laos
Authors: Tian Jinglin , Chen Tao , Zheng Jinyu , Wu Jiaxin , Xu Yating
Published: February 2023

The yellow seal stone from northern Laos is one possible substitute for the Tianhuang Stone, the most famous Chinese seal stone, because of its similar yellow to orange-yellow appearance and the same main mineral composition. The colour causation of the yellow seal stone from northern Laos was studied. The samples’ phase, micro-morphology and chemical components were studied by Raman spectroscopy, and scanning electron microscopy (SEM) with energy disperse spectroscopy (EDS), respectively. The yellow seal stone from northern Laos is mainly composed of dickite, occasionally with minor impurity minerals, such as hematite, anatase, barite, diaspore and pyrite. Micro- to nano-sized iron oxides/hydroxides were observed and detected by SEM and EDS in the yellow to orange-yellow part of the samples. Moreover, these iron oxides/hydroxides were suggested to cause the yellow to orange-yellow in the seal stone from northern Laos. The UV-Vis spectrum and its second derivative, the Kubelka-Munk spectra, were used to identify and quantify hematite and goethite. The samples’ colour parameters were obtained with the Commission Internationale de l’Eclairage (CIE) 1931 standard space. According to the observation of the samples and the results obtained from experiments and calculations, the colour of the yellow parts (L* = 33.56~47.99, a* = 0.35~3.65, b* = 4.55~9.89) correlated with goethite (goethite is about 0.175~0.671 g/kg, the content of hematite was too low to be figured out in the yellow parts). In contrast, the colour of the orange-yellow parts (L* = 33.99~46.27, a* = 3.98~12.39, b* = 8.04~22.14) was more closely related with the content of hematite (goethite is about 0.096~0.691 g/kg, hematite is about 0.258~2.383 g/kg). The results of correlation analysis also support that the contents of iron oxides or hydroxides influence the samples’ colour. Therefore, it is suggested that micro- to nano-scaled hematite and goethite caused the colour of yellow and orange-yellow in the studied seal stone. Hematite can strengthen the red hue and change the colour from yellow to orange-yellow.
Effect of Magnesium Sulfate Solution on Pore Structure of Ionic Rare Earth Ore during Leaching Process
Authors: Gao Zhongquan , Rao Yunzhang , Shi Liang , Xiang Run , Yang Zhihua
Published: February 2023

During in situ leaching of ionic rare earth ore, the pore structure of the orebody changes due to the chemical replacement reaction between the leaching agent and the rare earth ore. To explore the influence of leaching agents on the pore structure of ionic rare earth ore during the leaching process, magnesium sulfate solutions with different concentrations and pH are used as leaching agents in this paper. An experimental method of indoor simulated column leaching, a Zetaprobe potential analyzer, and an NM-60 rock microstructure analyzer to measure parameters, including surface zeta potential, T2 map, and the pore structure of rare-earth ore particles, were used to analyze the influence law of magnesium sulfate solution on the pore structure of ionic rare earth ore. The result proves that pure H2O leaching has little effect on the surface Zeta potential and the internal pore structure of the ore particles. In the leaching process of magnesium sulfate solutions with different concentrations, the absolute value of Zeta potential decreases, and the internal pore structure evolves from medium, large, and extra-large to small pores. In the leaching process of magnesium sulfate solutions with different pH, the absolute value of Zeta potential decreases and then increases slightly with the end of the ion exchange reaction. The internal pore structure generally shows a decrease in the number of small and extra-large pores and an increase in the number of medium and large pores. According to the analysis, the concentration and pH of the leaching agent cause the change of thickness of the electric double layer of the fine particles in the orebody, break the balance of interaction force between soil particles, and result in the evolution of a micropore structure of orebody during leaching.
Erucic Acid—Both Sides of the Story: A Concise Review on Its Beneficial and Toxic Properties
Authors: Galanty Agnieszka , Grudzińska Marta , Paździora Wojciech , Paśko Paweł
Published: February 2023

Erucic acid (EA) is monounsaturated fatty acid (22:1 n-9), synthesized in the seeds of many plants from the Brassicaceae family, with Brassica napus, B. rapa, or B. carinata considered as its richest source. As the compound has been blamed for the poisoning effect in Toxic Oil Syndrome, and some data indicated its cardiotoxicity to rats, EA has been for decades classified as toxic substance, the use of which should be avoided. However, the cardiac adverse effects of EA have not been confirmed in humans, and the experiments in animal models had many limitations. Thus, the aim of this review was to present the results of the so far published studies on both toxic, and pharmacological properties of EA, trying to answer the question on its future medicinal use. Despite the ambiguous and relatively small data on toxic and beneficial effects of EA it seems that the compound is worth investigating. Further research should be particularly directed at the verification EA toxicity, more in-depth studies on its neuroprotective and cytotoxic properties, but also its use in combination with other drugs, as well as its role as a drug carrier.
Novel 2-Thiouracil-5-Sulfonamide Derivatives: Design, Synthesis, Molecular Docking, and Biological Evaluation as Antioxidants with 15-LOX Inhibition
Authors: Ahmed Naglaa M. , Lotfallah Ahmed H. , Gaballah Mohamed S. , Awad Samir M. , Soltan Moustafa K.
Published: February 2023

New antioxidant agents are urgently required to combat oxidative stress, which is linked to the emergence of serious diseases. In an effort to discover potent antioxidant agents, a novel series of 2-thiouracil-5-sulfonamides (4–9) were designed and synthesized. In line with this approach, our target new compounds were prepared from methyl ketone derivative 3, which was used as a blocking unit for further synthesis of a novel series of chalcone derivatives 4a–d, thiosemicarbazone derivatives 5a–d, pyridine derivatives 6a–d and 7a–d, bromo acetyl derivative 8, and thiazole derivatives 9a–d. All compounds were evaluated as antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), lipid peroxidation, and 15-lipoxygenase (15-LOX) inhibition activity. Compounds 5c, 6d, 7d, 9b, 9c, and 9d demonstrated significant RSA in all three techniques in comparison with ascorbic acid and 15-LOX inhibitory effectiveness using quercetin as a standard. Molecular docking of compound 9b endorsed its proper binding at the active site pocket of the human 15-LOX which explains its potent antioxidant activity in comparison with standard ascorbic acid.