bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on the Influencing Mechanism of the Effect of Brands’ Sustainable Behaviors on Consumer Attitudes: An Empirical Study on Clothing Brands
Authors: Zhang Yiling , Zhou Ying , Liu Yangyang , Xiao Zengrui
Published: January 2023

Brands often link themselves with sustainable behavior in brand content marketing to obtain more consumer recognition. However, in fact, in the communication between brands and consumers, consumers cannot effectively respond to any sustainable behaviors of clothing brands other than the sustainable behavior of materials. This results in failure to stimulate the expression of deep culture, which then affects the long-term development of clothing brands. This study integrates material space benefit and spiritual space empathy as the intermediary role, aiming to explore the impact of brand sustainable behaviors on consumer brand attitude from the perspective of consumers. The data come from interviews with 12 experts, and 314 useable responses were collected (34.08% male and 65.92% female). Through in-depth interview, coding, and category analysis, we obtain dimensions of brand sustainable behaviors and a hypothetical model of relationship between brand behavior and consumer attitudes. We then use Amos software to quantify and demonstrate the mechanism of action between variables in the model. Research results show that: (1) Brand sustainable behaviors that have significant impact on brand attitude can be divided into three dimensions, which are product long-life design, cultural inheritance and reconstruction, and fashion digital empowerment. (2) Product long-life design has a significant direct effect on material space benefit; cultural inheritance and reconstruction significantly affect spiritual space empathy; fashion digital empowerment significantly affects material space benefit and spiritual space empathy. (3) Material space benefit and spiritual space empathy play a mediating role in the impact of brand sustainable behaviors on consumer attitudes, and the effect of spiritual space empathy on consumer brand attitudes has a high validity. The findings offer suggestions for sustainable strategies and content marketing of clothing brands, promotes effective communication between brands and consumers at the sustainable level, and help brands practice sustainability in normalization.
Construction Site Hazards Identification Using Deep Learning and Computer Vision
Authors: Alateeq Muneerah M. , P.P. Fathimathul Rajeena , Ali Mona A. S.
Published: January 2023

Workers on construction sites face numerous health and safety risks. Authorities have made numerous attempts to enhance safety management; yet incidents continue to occur, impacting both worker health and the project’s forward momentum. To that end, developing strategies to improve construction site safety management is crucial. The goal of this project is to employ computer vision and deep learning methods to create a model that can recognize construction workers, their PPE and the surrounding heavy equipment from CCTV footage. Then, the hazards can be discovered and identified based on an analysis of the imagery data and other criteria including weather conditions, and the on-site safety officer can be contacted. Our own dataset was used to train the You Only Look Once model, version 5 (YOLO-v5), which was put to use as an object detection model. The detection model’s performance in tests showed promise for fast and accurate object recognition in the field.
Local Food Systems under Global Influence: The Case of Food, Health and Environment in Five Socio-Ecosystems
Authors: Rapinski Michael , Raymond Richard , Davy Damien , Herrmann Thora , Bedell Jean-Philippe , Ka Abdou , Odonne Guillaume , Chanteloup Laine , Lopez Pascal Jean , Foulquier Éric , da Silva Eduardo Ferreira , El Deghel Nathalie , Boëtsch Gilles , Coxam Véronique , Joliet Fabienne , Guihard-Costa Anne-Marie , Tibère Laurence , Nazare Julie-Anne , Duboz Priscilla
Published: January 2023

Globalization is transforming food systems around the world. With few geographical areas spared from nutritional, dietary and epidemiological transitions, chronic diseases have reached pandemic proportions. A question therefore arises as to the sustainability of local food systems. The overall purpose of this article is to put in perspective how local food systems respond to globalization through the assessment of five different case studies stemming from an international research network of Human-Environment Observatories (OHM), namely Nunavik (Québec, Canada), Oyapock (French Guiana, France), Estarreja (Portugal), Téssékéré (Senegal) and Littoral-Caraïbes (Guadeloupe, France). Each region retains aspects of its traditional food system, albeit under different patterns of influence modelled by various factors. These include history, cultural practices, remoteness and accessibility to and integration of globalized ultra-processed foods that induce differential health impacts. Furthermore, increases in the threat of environmental contamination can undermine the benefits of locally sourced foods for the profit of ultra-processed foods. These case studies demonstrate that: (i) the influence of globalization on food systems can be properly understood by integrating sociohistorical trajectories, socioeconomic and sociocultural context, ongoing local environmental issues and health determinants; and (ii) long-term and transverse monitoring is essential to understand the sustainability of local food systems vis-à-vis globalization.
Networked Learning Communities in Promoting Teachers’ Receptivity to Change: How Professional Learning Beliefs and Behaviors Mediate
Authors: Pan Hui-Ling Wendy , Chen Wen-Yan
Published: January 2023

More research on networked learning communities (NLCs) and the causal mechanism among the effects of NLCs are needed. To better understand the impacts of NLCs and the influential factors, this study intended to discover how teachers’ participation in networked learning communities affects their beliefs and behaviors of professional learning and further influence their receptivity to change. Adopting a survey design, we collected 226 valid questionnaires from the pilot schools joining the program of Learning Community under Leadership for Learning supported by the Ministry of Education in Taiwan. First, the results indicated that the program’s intervention of NLCs had a significant positive effect on teachers’ receptivity to change. Second, teachers’ participation in NLCs also showed a significant impact on their beliefs and behaviors regarding professional learning. Third, teachers’ beliefs in professional collaborative learning could significantly enhance their behaviors of professional collaborative learning. Fourth, the program’s intervention, employing hands-on professional learning activities, had a significant impact on teachers’ inclination to realize the program, both through direct influence and the mediation of beliefs about professional learning. The results acquired from this study would be conducive to developing strategies to support implementing the NLCs program.
An Integrated PCA–AHP Method to Assess Urban Social Vulnerability to Sea Level Rise Risks in Tampa, Florida
Authors: Xie Weiwei , Meng Qingmin
Published: January 2023

Coastal flooding risks are increasing with the rise of sea level due to global climate change. Such risk presents different groups of residents with diverse vulnerability. Using a coastal city as the study area, this study quantitatively and qualitatively examines social vulnerability across different communities at risk of sea level rising. This study develops a novel social vulnerability assessment method that integrates principal component analysis (PCA) and analytical hierarchical process (AHP), inputting the advantages of each into factor analysis for social vulnerability quantification. Twenty-four socioeconomic factors are first grouped into four different themes. PCA is then performed to identify major components within each theme. We apply AHP to generate the weighting scheme for each theme. Therefore, the AHP-derived weights to those PCA components according to each theme are used to obtain an overall social vulnerability index. The thematic map of PCA–AHP SVI showed that minority communities with low income, mobile home, and unemployed populations aggregated in the East Tampa shore (over 69% of the total minority), compared with the West Tampa (31% of the total minority). Our findings provide insightful spatial information of the social vulnerability index (SVI) that allows decision makers to make optimal efforts to mitigate damages and unexpected impacts on different communities, especially those marginalized minorities due to sea level rising risks.
Influences of Flow Channel on Electrochemical Characteristics of Polymer Electrolyte Fuel Cells Humidified with NaCl Contained H2O
Authors: Yoo Ho Jun , Cho Gu Young
Published: January 2023

In this study, the effects of flow field types on the electrochemical properties of polymer electrolyte membrane fuel cells (PEMFCs) humidified with NaCl solution are systematically investigated. The parallel flow field and serpentine flow field were used to investigate the PEMFCs. Long-term stability was evaluated for 20 h using chronoamperometry. Fuel cells with both parallel and serpentine flow fields showed a decrease in performance because of the NaCl solution. Interestingly, the PEMFC with the serpentine flow field showed significantly more severe degradation during long-term stability evaluation compared to the fuel cell with the parallel flow field. Electrochemical impedance spectroscopy analysis showed that a significant increase in faradaic resistance caused the degradation of the performance. After long-term stability examinations, regenerations of fuel cells were performed with deionized water at a constant voltage (0.4 V). After the regeneration, the performance of the fuel cells with the serpentine flow field was improved more (52.96%) than the PEMFC with the parallel flow field (1.22%).
Sustainable Operation of Fine-Dining Restaurants: Antecedents and Consequences of Customers’ Self-Image Congruity at a Cantonese Michelin-Starred Restaurant Based on the Value-Attitude-Behavior Model
Authors: Liu Si-Fan , Li Zhi-Xuan , Zhang Yang
Published: January 2023

With the current rapid economic development, restaurant practitioners need to pay attention to the issue of how fine-dining restaurants can achieve sustainable operations in the presence of fierce competition. Fine-dining restaurants have gradually become a reflection of consumers’ self-image; therefore, this study combines the VAB framework, self-congruity theory, and generational theory to investigate the relationships among perceived quality, customers’ self-image congruity, and their willingness to pay a price premium (WTP-PP). Current research uses generation as a moderator to explore the intergenerational differences between Gen X and Gen Y. We adopted Smart-PLS to conduct SEM and MGA. The results of this study showed that the quality of the atmosphere and food induced actual, ideal, and ideal social self-image congruity, while the quality of the service could not only induce the above three aspects of self-consistency but also induce social self-image congruity and have a significant positive impact on WTP-PP. Meanwhile, WTP-PP was also significantly affected by actual self-image congruity and ideal self-image congruity. Furthermore, Gen Yers cared more about the atmosphere quality than Gen X. Contrarily, Gen Xers valued food quality more than Gen Y.
Emergency Response to the COVID-19 Pandemic of the King Abdulaziz University in Jeddah: A Report on Stakeholder’s Opinions
Authors: Aloufi Fahed A. , Taleb Md. Abu , Halawani Riyadh F. , Tammar Abdurazag , Mahmood Shreef , Rahaman Khan Rubayet
Published: January 2023

The devastating effect of COVID-19 has impacted global citizens for the past three years. More than six hundred and forty-six million people have been infected and there have been almost seven million casualties. Consequently, new variants have been discovered in quick succession around the world. Global communities have witnessed cruel fatalities and lost properties and businesses, and experienced the usual activities of service sectors being hampered, including those of post-secondary educational institutions, and the consequences of the COVID-19 pandemic ultimately damaged family life and society in general. Emergency management strategies were adopted by educational institutions around the world, including in the Middle East, in order to manage the ongoing pandemic. This study aimed to evaluate the emergency response mechanisms to COVID-19 at the King Abdulaziz University (KAU) by interviewing major stakeholders to ascertain their opinions through a cross-sectional survey. A total of 350 responses were recorded from students (64.28%), faculty members (21.42), and staff (14.28). The collected data were analyzed using statistical methods and illustrated using different schemes, graphs, and diagrams. Interestingly, the KAU emergency response plan for COVID-19 was appreciated by the respondents and it has emerged as a success story at a post-secondary educational institution in the KSA.
A Systematic Review of the Impact of Physical Activity on Cognitive and Noncognitive Development in Chinese University Students
Authors: Zhang Hanying , Qin Yuke , Khalid Sabika , Tadesse Endale , Gao Chunhai
Published: January 2023

A large volume of empirical evidence and literature reviews on physical activity have concentrated on primary and secondary education. Thus, the primary aim of this systematic review is to synthesize empirical evidence regarding the potential impact of physical activity on academic and nonacademic performance among Chinese university students. A search and analysis of studies was conducted in accordance with the PRISMA guidelines by using an electronic database search (PubMed, Science Direct, and Scopus), and 150 studies were obtained. Subsequently, after producing rigorous inclusion and exclusion criteria, which were used to consider quality, methodology, language, geographical location, year of publication, etc., a total of 11 quantitative research articles, based on Chinese higher education institutions, were chosen for synthesis. The comprehensive analysis of the studies affirmed that physical activity dramatically benefits university students by fostering their nonacademic skills, such as social relatedness, life satisfaction, and mental health, in addition to boosting their academic achievement in terms of graduation, GPA, academic motivation, and engagement. However, the aim of this systematic review was to provide implications for future scholars to explicitly study university students’ educational level, discipline, sex, and type of physical activity.
Improving NoSQL Spatial-Query Processing with Server-Side In-Memory R*-Tree Indexes for Spatial Vector Data
Authors: Sun Lele , Jin Baoxuan
Published: January 2023

Geospatial databases are basic tools to collect, index, and manage georeferenced data indicators in sustainability research for efficient, long-term analysis. NoSQL databases are increasingly applied to manage the ever-growing massive spatial vector data (SVD) with their changeable data schemas, agile scalability, and fast query response time. Spatial queries are basic operations in geospatial databases. According to Green information technology, an efficient spatial index can accelerate query processing and save power consumption for ubiquitous spatial applications. Current solutions tend to pursue it by indexing spatial objects with space-filling curves or geohash on NoSQL databases. As for the performance-wise R-tree family, they are mainly used in slow disk-based spatial access methods on NoSQL databases that incur high loading and searching costs. Therefore, performing spatial queries efficiently with the R-tree family on NoSQL databases remains a challenge. In this paper, an in-memory balanced and distributed R*-tree index named the BDRST index is proposed and implemented on HBase for efficient spatial-query processing of massive SVD. The BDRST index stores and distributes serialized R*-trees to HBase regions in association with SVD partitions in the same table. Moreover, an efficient optimized server-side parallel processing framework is presented for real-time R*-tree instantiation and query processing. Through extensive experiments on real-world land-use data sets, the performance of our method is tested, including index building, index quality, spatial queries, and applications. Our proposed method outperforms other state-of-the-art solutions, saving between 27.36% and 95.94% on average execution time for the above operations. Experimental results show the capability of the BDRST index to support spatial queries over large-scale SVD, and our method provides a solution for efficient sustainability research that involves massive georeferenced data.
Remarks on the Concept of a Sustainable City in Light of Konrad Lorenz’s Concept of Man
Authors: Sadowski Ryszard F.
Published: January 2023

For several decades now, intensive research has been carried out on the concept of a city that would be as environmentally friendly as possible, while simultaneously providing a high quality of life for its residents. As a result of this research, the concept of a sustainable city that addresses these challenges has been developed. The significance of this issue is evidenced by the fact that Sustainable Development Goal 11 is explicitly concerned with this topic. This paper aims to analyze the concept of a sustainable city from the perspective of the so-called ‘layered’ concept of man authored by Konrad Lorenz. It is based on the observation that the pace of biological evolution and that of the cultural evolution man undergoes differ significantly. While humans have adapted very quickly to urban living in their cultural layer, adaptation to city life in their biological layer has been very slow. The natural way of life pursued by the Homo sapiens was a hunter–gatherer lifestyle, which biological evolution had adapted the species to over hundreds of thousands of years. The changes associated with the Neolithic Revolution and the formation of the first cities took place about 11,000 years ago. Thus, not enough time has elapsed for humans to adapt biologically to their radically changed living conditions. The purpose of this paper is to look from a new perspective at the challenges faced by city dwellers, and in doing so, to identify the sources of these challenges and to arrange them in a hierarchy. An additional goal is to expand the argument for promoting certain elements of a sustainable city. Indeed, much seems to suggest that Lorenz’s concept offers a novel approach to looking at such urban issues as the challenges of overpopulation, man’s restricted access to nature, and the ever-increasing pace of life.
The Internalization of Participation and Coherence Dimensions of Governance in Tourism Destination Management Organizations—An Exploratory Approach
Authors: Bono i Gispert Octavi , Anton Clavé Salvador , Casadesús Fa Martí
Published: January 2023

This exploratory research aims to determine how governance is internalized in tourism destinations management organizations (DMO). Governance internalization is understood as the commitment of destination management-related organizations to the deployment of governance concepts, precepts, practices, and actions and the rolling out and exercise of their functions, responsibilities, and power. The methodology that was used is that of case studies. A total of eight organizations acknowledging different degrees of governance commitment have been analyzed through in-depth interviews and documentary reviews. For each case, two dimensions of governance—participation and coherence—have been analyzed. It has been observed how organizations with a higher or lower degree of commitment show clear equivalence between their governance vision and the level of internalization of the participation and coherence dimensions. However, a rhetorical implementation of governance concepts, precepts, practices, and actions is found among destinations with more lukewarm positions.
Fresh, Hardened, and Microstructural Properties of Ambient Cured One-Part Alkali-Activated Self-Consolidating Concrete
Authors: Kanaan Dima , Safhi Amine el Mahdi , Suleiman Ahmed R. , Soliman Ahmed M.
Published: January 2023

Several studies have investigated the properties of alkali-activated materials (AAM), considering it as a substitute of cementitious concrete. However, the studies on alkali-activated self-consolidating concrete (AASCC) are extremely limited. This paper investigated the properties of AASCCs utilizing ground granulated blast furnace slag (GGBFS) as the main precursor. Single, binary, and ternary AASCCs were produced using fly ash Class-F (FA) and silica fumes (SF) as a replacement for GGBFS. The fresh properties including filing ability, passing ability and stability, as well as the hardened properties including unconfined compressive strength, ultrasonic pulse velocity, electrical resistivity, absorption, and sorptivity of the ambient cured one-part AASCC mixtures with different precursor blends were investigated. In addition, the microstructural properties of 90-day AASCC blends were studied by various microscale analysis methods. This paper demonstrated that the higher fraction of sodium carbonate/silicate activators, ranging from 20% to 25%, contributed to delayed reaction kinetics and satisfactory fresh and mechanical properties in all systems due to their nature. Slag replacement with variable SF or FA class-F ratios, instead, could indeed adjust the particle size distribution of the total binder material and improve the fresh concrete characteristics in binary and ternary systems. Finally, the formation of various reaction products and binding gels, i.e., C-(N)A-S-H, was found to have a significant impact on several transport mechanisms, including capillary sorptivity, permeable pores, and bulk electrical resistivity.
Sustainable Adsorbent Material Prepared by Soft Alkaline Activation of Spent Coffee Grounds: Characterisation and Adsorption Mechanism of Methylene Blue from Aqueous Solutions
Authors: Cuccarese Marco , Brutti Sergio , De Bonis Angela , Teghil Roberto , Di Capua Francesco , Mancini Ignazio Marcello , Masi Salvatore , Caniani Donatella
Published: January 2023

Dyes are emerging as hazardous pollutants, which are the primary challenges for environmentalists. Dye removal from effluents is urgently needed. Adsorption technology has been widely employed in this context as an effective method for removing colours from the aqueous phase, and adsorption with the use of low-cost adsorbents has been shown to be more successful on a larger scale than other methods. In this study, spent coffee grounds (SCGs) were used as the precursor for the preparation of a low-cost activated carbon through the chemical activation with NaOH. The SCG sample was impregnated with NaOH and carbonised at 300 °C for three hours. Its morphological and physical-chemical properties were assessed using scanning electron microscopy (SEM), X-ray diffraction, and Raman spectroscopy analyses. The performance of the treated SCG as an adsorbent material for methylene blue (MB) was evaluated by analysing the effect of the initial pH ionic strength on the adsorption capacity and by evaluating the kinetics and the mechanisms of the process (using adsorption isotherms). The effect of the initial concentration (500 and 250 mg L−1) of MB on the kinetics of the process and the impact of the initial pH (7.5 and 6) on the adsorption isotherm were evaluated. The obtained results show that the pseudo-second order model controls the process for both the investigated initial concentration and the adsorption capacity, which are 142.8 and 113.6 mg L−1, respectively. The results indicate that the pH value influences the adsorption isotherm model that regulates the process. Specifically, this process is regulated by the Temkin’s model with a pH of 7.5 and by the Langmuir’s model with a pH of 6. The thermodynamics of the process were also determined. The results show that SCG, treated and carbonised by soft alkaline activation, is a promising low-cost adsorbent material as its performance is comparable to that of conventional active carbon materials.
FSPV-Grid System for an Industrial Subsection with PV Price Sensitivity Analysis
Authors: Rizvi Tanu , Dubey Satya Prakash , Tripathi Nagendra , Srivastava Gautam , Makhija Satya Prakash , Mohiddin Md. Khaja
Published: January 2023

Renewable energy sources, particularly solar photovoltaic generation, now dominate generation options. Solar generation advancements have resulted in floating solar photovoltaics, also known as FSPV systems. FSPV systems are one of the fastest growing technologies today, providing a viable replacement for ground-mounted PV systems due to their flexibility and low land-space requirement. This paper presents a systematic approach for implementing a proposed FSPV–grid integrated system in Bhilai Steel Plant’s (BSP) subsections. BSP is a steel manufacturing plant located in Bhilai, Chhattisgarh, and the FSPV system has the potential to generate sufficient energy by accessing two of its reservoirs. The system was simulated in HOMER Pro software, which provided the FSPV system power estimations, area requirements, net present cost (NPC), levelized cost of energy (LCOE), production summary, grid purchasing/selling, IRR, ROI, paybacks and pollutant emissions. A sensitivity analysis for a hike in PV prices globally due to a shortage in poly silicone in international markets during the fiscal year 2021–2022 was undertaken for the proposed FSPV–grid system. Here, the authors considered hikes in the PV price of 1%, 9%and 18% respectively, since the maximum percentage increase in PV prices globally is 18%. The authors also compared the proposed FSPV–grid system to the existing grid-only system for two sections of the BSP and the results obtained showed that the NPC and LCOE would be much lower in the case of the FSPV–grid system than the grid-only system. However, with changes in the percentage hike in PV prices, the NPC and LCOE were found to increase due to changes in the proportion of FSPV–grid systems in production. The pollutant emissions were the minimum in the case of the FSPV–grid system, whereas they were the highest in the case of the existing grid-only system. Furthermore, the payback analysis indicated that the minimum ROI for the above-defined construction would be fully covered in 15.81 years with the nominal 1% pricing for FSPV–grid generation. Therefore, the overall results suggest that the FSPV–grid system has the potential to be a perfect alternative solar energy source that can meet the current electrical energy requirements of the steel manufacturing industry with nominal pricing better than the existing grid-only system, as well as addressing economic constraints and conferring environmental benefits.
Combination of Metaheuristic Optimization Algorithms and Machine Learning Methods for Groundwater Potential Mapping
Authors: AlAyyash Saad , Al-Fugara A’kif , Shatnawi Rania , Al-Shabeeb Abdel Rahman , Al-Adamat Rida , Al-Amoush Hani
Published: January 2023

The groundwater contained in aquifers is among the most important water supply resources, especially in semi-arid and arid regions worldwide. This study aims to evaluate and compare the prediction capability of two well–known models, support vector machine (SVM) and adaptive neuro-fuzzy inference system (ANFIS), combined with a genetic algorithm (GA), invasive weed optimization (IWO), and teaching–learning-based optimization (TLBO) algorithms in groundwater potential mapping (GPM) the Azraq Basin in Jordan. The hybridization of the SVM and ANFIS models with the GA, IWO, and TLBO algorithms results in six models: SVM–GA, SVM–IWO, SVM–TLBO, ANFIS–GA, ANFIS–IWO, and ANFIS–TLBO. A database consisting of well data containing 464 wells with 12 predictive factors was developed for the groundwater potential mapping (GPM) of the study area. Of the 464 well locations, 70% (325 locations) were assigned for the training set and the rest (139 locations) for the validation set. The correlation between the 12 predictive factors and the well locations is analyzed using the frequency ratio (FR) statistical model. An area under receiver operating characteristic (AUROC) curve was used to evaluate and compare the models. According to the results, the SVM-based hybrid models outperformed other ANFIS hybrid models in the learning (training) and validation phases. The SVM–GA and SVM–TLBO hybrid models showed AUROC values of 0.984 and 0.971, respectively, in the training and validation phases. Moreover, the ANFIS–GA and ANFIS–TLBO hybrid models showed an AUROC of 0.979 and 0.984 in the training phase and an AUROC of 0.973 and 0.984 in the validation phase, respectively. The SVM–IWO and ANFIS–IWO hybrid models showed the lowest AUROC. This study demonstrated the more efficient results of the SVM-based hybrid models in comparison with the ANFIS-based hybrid models in terms of accuracy and modeling speed.
Experimental Evidence Questions the Relationship between Stress and Fluctuating Asymmetry in Plants
Authors: Gavrikov Dmitry E. , Zverev Vitali , Rachenko Maksim A. , Pristavka Aleksey A. , Kozlov Mikhail V.
Published: January 2023

The eco-evolutionary theory of developmental instability predicts that small, non-directional deviations from perfect symmetry in morphological traits (termed fluctuating asymmetry, FA) emerge when an individual is unable to buffer environmental or genetic stress during its development. Consequently, FA is widely used as an index of stress. The goal of the present study was to experimentally test a seemingly trivial prediction derived from the theory of developmental instability—and from previous observational studies—that significant growth retardation (which indicates stress) in plants is accompanied by an increase in FA of their leaves. We induced stress, evidenced by a significant decrease in biomass relative to control, in cucumber (Cucumis sativus), sweet pepper (Capsicum annuum), and common bean (Phaseolus vulgaris) by applying water solutions of copper and nickel to the soil in which plants were grown. Repeated blind measurements of plant leaves revealed that leaf FA did not differ between stressed and control plants. This finding, once again, demonstrated that FA cannot be seen as a universal indicator of environmental stress. We recommend that the use of FA as a stress index is discontinued until the scope of the developmental instability theory is clarified and its applicability limits are identified.
On the Logical Structure of Composite Symmetries in Atomic Nuclei
Authors: Cseh József
Published: January 2023

This investigation involves composite symmetries, which connect different components of atomic nuclei. In particular, supersymmetry (SUSY) connects boson and fermion sectors, and multiconfigurational symmetry (MUSY) bridges different configurations, such as shell, quartet, or cluster configurations. Both SUSY and MUSY contain a usual dynamical symmetry in each component, and further symmetry connects the components to each other. The varieties of the connecting symmetries and their logical structure are analyzed and compared.
Dual Magnetic Particle Imaging and Akaluc Bioluminescence Imaging for Tracking Cancer Cell Metastasis
Authors: Williams Ryan J. , Sehl Olivia C. , Gevaert Julia J. , Liu Shirley , Kelly John J. , Foster Paula J. , Ronald John A.
Published: January 2023

Magnetic particle imaging (MPI) provides hotspot tracking and direct quantification of superparamagnetic iron oxide nanoparticle (SPIO)-labelled cells. Bioluminescence imaging (BLI) with the luciferase reporter gene Akaluc can provide complementary information on cell viability. Thus, we explored combining these technologies to provide a more holistic view of cancer cell fate in mice. Akaluc-expressing 4T1Br5 cells were labelled with the SPIO Synomag-D and injected into the mammary fat pads (MFP) of four nude mice. BLI was performed on days 0, 6 and 13, and MPI was performed on days 1, 8 and 14. Ex vivo histology and fluorescence microscopy of MFP and a potential metastatic site was conducted. The BLI signal in the MFP increased significantly from day 0 to day 13 (p < 0.05), mirroring tumor growth. The MPI signal significantly decreased from day 1 to day 14 (p < 0.05) due to SPIO dilution in proliferating cells. Both modalities detected secondary metastases; however, they were visualized in different anatomical regions. Akaluc BLI complemented MPI cell tracking, allowing for longitudinal measures of cell viability and sensitive detection of distant metastases at different locations. We predict this multimodal imaging approach will help to evaluate novel therapeutics and give a better understanding of metastatic mechanisms.
Concurrent Infection with SARS-CoV-2 and Orientia tsutsugamushi during the COVID-19 Pandemic in the Maldives
Authors: Dey Rajib Kumar , Imad Hisham Ahmed , Aung Pyae Linn , Faisham Mohamed , Moosa Muaz , Hasna Mariyam , Afaa Aminath , Ngamprasertchai Thundon , Matsee Wasin , Nguitragool Wang , Nakayama Emi E. , Shioda Tatsuo
Published: January 2023

The COVID-19 pandemic was the worst public-health crisis in recent history. The impact of the pandemic in tropical regions was further complicated by other endemic tropical diseases, which can cause concurrent infections along with COVID-19. Here, we describe the clinical course of a patient with concurrent COVID-19 and scrub typhus infection. The patient’s de-identified clinical data were retrieved retrospectively. The patient had progressive breathlessness at the time of presentation and was hospitalized for COVID-19. Respiratory examination revealed dyspnea, tachypnea, and coarse crepitations bilaterally over the entire lung field. Oxygenation was impaired, and a PaO2/FiO2 ratio of 229 suggested acute respiratory distress syndrome. Laboratory tests indicated leukocytosis, thrombocytopenia, ferritinemia, hypoalbuminemia, and transaminitis. Upon revaluation for persistent fever, physical examination revealed an eschar in the right antecubital fossa. Serology further confirmed scrub typhus, with IgM and IgG antibody positivity. A remarkable clinical recovery was achieved with doxycycline. The COVID-19 pandemic might have masked endemic tropical diseases. Clinicians working in endemic regions must always consider common tropical diseases that may present as a co-infection, as in our case. Travel and exposure history are critical guides for narrowing down a differential diagnosis. Early diagnosis and treatment can prevent complications.
Extracellular Vesicles Are Conveyors of the NS1 Toxin during Dengue Virus and Zika Virus Infection
Authors: Safadi Daed El , Lebeau Grégorie , Lagrave Alisé , Mélade Julien , Grondin Lauriane , Rosanaly Sarah , Begue Floran , Hoareau Mathilde , Veeren Bryan , Roche Marjolaine , Hoarau Jean-Jacques , Meilhac Olivier , Mavingui Patrick , Desprès Philippe , Viranaïcken Wildriss , Krejbich-Trotot Pascale
Published: January 2023

Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs and exosomes in particular have the natural ability to transport nucleic acids, proteins, and other components of cellular or viral origin. Thus, they participate in intercellular communication, immune responses, and infectious and pathophysiological processes. Some viruses are known to hijack the cell production and content of EVs for their benefit. Here, we investigate whether two pathogenic flaviviruses i.e., Zika Virus (ZIKV) and Dengue virus (DENV2) could have an impact on the features of EVs. The analysis of EVs produced by infected cells allowed us to identify that the non-structural protein 1 (NS1), described as a viral toxin, is associated with exosomes. This observation could be confirmed under conditions of overexpression of recombinant NS1 from each flavivirus. Using different isolation methods (i.e., exosome isolation kit, size exclusion chromatography, Polyethylene Glycol enrichment, and ELISA capture), we showed that NS1 was present as a dimer at the surface of excreted exosomes, and that this association could occur in the extracellular compartment. This finding could be of major importance in a physiological context. Indeed, this capacity of NS1 to address EVs and its implication in the pathophysiology during Dengue or Zika diseases should be explored. Furthermore, exosomes that have demonstrated a natural capacity to vectorize NS1 could serve as useful tools for vaccine development.
Human Arboviral Infections in Italy: Past, Current, and Future Challenges
Authors: Rossi Benedetta , Barreca Filippo , Benvenuto Domenico , Braccialarghe Neva , Campogiani Laura , Lodi Alessandra , Aguglia Camilla , Cavasio Rosario Alessandro , Giacalone Maria Laura , Kontogiannis Dimitra , Moccione Martina , Malagnino Vincenzo , Andreoni Massimo , Sarmati Loredana , Iannetta Marco
Published: January 2023

Arboviruses represent a public health concern in many European countries, including Italy, mostly because they can infect humans, causing potentially severe emergent or re-emergent diseases, with epidemic outbreaks and the introduction of endemic circulation of new species previously confined to tropical and sub-tropical regions. In this review, we summarize the Italian epidemiology of arboviral infection over the past 10 years, describing both endemic and imported arboviral infections, vector distribution, and the influence of climate change on vector ecology. Strengthening surveillance systems at a national and international level is highly recommended to be prepared to face potential threats due to arbovirus diffusion.
Recombinant Pseudorabies Virus Usage in Vaccine Development against Swine Infectious Disease
Authors: Zhou Mo , Abid Muhammad , Cao Shinuo , Zhu Shanyuan
Published: January 2023

Pseudorabies virus (PRV) is the pathogen of pseudorabies (PR), which belongs to the alpha herpesvirus subfamily with a double stranded DNA genome encoding approximately 70 proteins. PRV has many non-essential regions for replication, has a strong capacity to accommodate foreign genes, and more areas for genetic modification. PRV is an ideal vaccine vector, and multivalent live virus-vectored vaccines can be developed using the gene-deleted PRV. The immune system continues to be stimulated by the gene-deleted PRVs and maintain a long immunity lasting more than 4 months. Here, we provide a brief overview of the biology of PRV, recombinant PRV construction methodology, the technology platform for efficiently constructing recombinant PRV, and the applications of recombinant PRV in vaccine development. This review summarizes the latest information on PRV usage in vaccine development against swine infectious diseases, and it offers novel perspectives for advancing preventive medicine through vaccinology.
Identifying Longitudinal CD4:CD8 Ratio Trajectories Indicative of Chronic Renal Disease Risk among People Living with HIV: An Application of Growth Mixture Models
Authors: Fonseca-Cuevas Alejandra , Newsome Patrick , Wang Lu , Chen Michelle Y. , Richardson Chris G. , Hull Mark , McLinden Taylor , Guillemi Silvia , Barrios Rolando , Montaner Julio S. G. , Lima Viviane D.
Published: January 2023

The incidence of chronic kidney disease (CKD) is increasing among people living with HIV (PLWH). Routine monitoring of indicators such as CD4:CD8 ratio might improve the early detection of CKD. Our objective was to identify clinically relevant CD4:CD8 ratio trajectories indicative of CKD risk. Participants were ≥ 18 years old, initiated antiretroviral therapy between 2000 and 2016, and were followed for ≥6 months until 31 March 2017 or last contact date. Outcome was incidence of CKD. Growth mixture models (GMMs) and decay models were used to compare CD4:CD8 ratio trajectories. Following GMM, 4547 (93.5%) participants were classified in Class 1 with 5.4% developing CKD, and 316 (6.5%) participants were classified in Class 2 with 20.9% developing CKD. The final model suggested that participants in Class 2 had 8.72 times the incidence rate of developing CKD than those in Class 1. Exponential decay models indicated a significant CD4:CD8 ratio decline among Class 2 participants who developed CKD. Among those who developed CKD in Class 2, starting at 5.5 years of follow-up, the slope of their ratio trajectory curve changed significantly, and the rate of decline increased dramatically. Routine monitored CD4:CD8 ratios can be an effective strategy to identify early CKD risk among PLWH.
Prevalence of Barmah Forest Virus, Chikungunya Virus and Ross River Virus Antibodies among Papua New Guinea Military Personnel before 2019
Authors: Kizu Joanne G. , Graham Melissa , Grant Richard , McCallum Fiona , McPherson Brady , Auliff Alyson , Kaminiel Peter , Liu Wenjun
Published: January 2023

Barmah Forest virus (BFV), Chikungunya virus (CHIKV) and Ross River virus (RRV) belong to the Alphavirus genus of the family Togaviridae. All three virus infections have been reported in Papua New Guinea (PNG) previously, but the exact prevalence and distribution of these three alphaviruses in PNG has not been established. Sera collected from 204 PNG Military Personnel (PNGMP) study participants in April 2019 was tested for the presence of anti-BFV, anti-CHIKV and anti-RRV immunoglobulin G (IgG) antibodies using commercially available enzyme-linked immunosorbent assay (ELISA) IgG detection kits, as well as for specific neutralizing antibodies (NAb) against individual viruses. Overall, sero-positivity of the sera was anti-BFV IgG 12.3% (25/204), anti-BFV NAb 8.3% (17/204); anti-CHIKV IgG 47.1% (96/204), anti-CHIKV NAb 34.8% (71/204); and anti-RRV IgG 93.1% (190/204), anti-RRV NAb 56.4% (115/204), respectively. Of the 137/204 participants that were Nab-positive for at least one virus, we identified 4 BFV, 40 CHIKV and 73 RRV single infections, and 9 RRV+CHIKV and 11 BFV+RRV double infections. The lower proportion of NAb sero-positive compared to the ELISA IgG sero-positive assay samples suggests that the currently available commercial ELISA detection kits for these three alphaviruses may not be suitable for diagnostic/surveillance purposes in endemic areas such as PNG, due to serological cross-reactivity among these three alphaviruses. Laboratory testing using known positive control sera indicated no cross-neutralization between BFV and RRV; however, some RRV or BFV single infection human sera demonstrated low-level cross-neutralization against CHIKV (the ratio of RRV/CHIKV NAb titers or BFV/CHIKV ≥ 4). Our preliminary results indicate that the majority of PNGMP have previously been exposed to RRV, with mild exposure to CHIKV and low-level exposure to BFV, suggesting that multiple alphaviruses have been circulating among PNGMP. The transmission landscapes of these three alphaviruses across PNG should be prioritized for further investigation, including identification of specific vectors and hosts that mediate human spillover in order to mitigate future outbreaks. Ongoing education regarding precautionary and protective measures are needed to better protect individuals who travel to PNG.
The Metagenomic Analysis of Viral Diversity in Colorado Potato Beetle Public NGS Data
Authors: Starchevskaya Maria , Kamanova Ekaterina , Vyatkin Yuri , Tregubchak Tatyana , Bauer Tatyana , Bodnev Sergei , Rotskaya Ulyana , Polenogova Olga , Kryukov Vadim , Antonets Denis
Published: January 2023

The Colorado potato beetle (CPB) is one of the most serious insect pests due to its high ecological plasticity and ability to rapidly develop resistance to insecticides. The use of biological insecticides based on viruses is a promising approach to control insect pests, but the information on viruses which infect leaf feeding beetles is scarce. We performed a metagenomic analysis of 297 CPB genomic and transcriptomic samples from the public National Center for Biotechnology Information Sequence Read Archive (NCBI SRA) database. The reads that were not aligned to the reference genome were assembled with metaSPAdes, and 13314 selected contigs were analyzed with BLAST tools. The contigs and non-aligned reads were also analyzed with Kraken2 software. A total of 3137 virus-positive contigs were attributed to different viruses belonging to 6 types, 17 orders, and 32 families, matching over 97 viral species. The annotated sequences can be divided into several groups: those that are homologous to genetic sequences of insect viruses (Adintoviridae, Ascoviridae, Baculoviridae, Dicistroviridae, Chuviridae, Hytrosaviridae, Iflaviridae, Iridoviridae, Nimaviridae, Nudiviridae, Phasmaviridae, Picornaviridae, Polydnaviriformidae, Xinmoviridae etc.), plant viruses (Betaflexiviridae, Bromoviridae, Kitaviridae, Potyviridae), and endogenous retroviral elements (Retroviridae, Metaviridae). Additionally, the full-length genomes and near-full length genome sequences of several viruses were assembled. We also found sequences belonging to Bracoviriform viruses and, for the first time, experimentally validated the presence of bracoviral genetic fragments in the CPB genome. Our work represents the first attempt to discover the viral genetic material in CPB samples, and we hope that further studies will help to identify new viruses to extend the arsenal of biopesticides against CPB.
Isolation, Characterization, and Transcriptome Analysis of an ISKNV-Like Virus from Largemouth Bass
Authors: Xu Zhuqing , Liao Jiaming , Zhang Dongzhuo , Liu Shaoli , Zhang Luhao , Kang Shaozhu , Xu Linting , Chen Hong , Peng Wenquan , Zhou Sheng , Qin Qiwei , Wei Jingguang
Published: January 2023

Largemouth bass (Micropterus salmoides) is an important commercial fish farmed in China. Challenges related to diseases caused by pathogens, such as iridovirus, have become increasingly serious. In 2017, we detected iridovirus-infected diseased largemouth bass in Zunyi, Guizhou Province. The isolated virus was identified as an infectious spleen and kidney necrosis virus (ISKNV)-like virus (ISKNV-ZY). ISKNV-ZY induces a cytopathic effect after infecting mandarin fish brain (MFB) cells. Abundant hexagonal virus particles were observed in the cytoplasm of ISKNV-ZY-infected MFB cells, using electron microscopy. The whole genome of ISKNV-ZY contained 112,248 bp and 122 open reading frames. Phylogenetic tree analysis showed that ISKNV-ZY was most closely related to BCIV, indicating that it is an ISKNV-like megalocytivirus. ISKNV-ZY-infected largemouth bass started to die on day six and reached a death peak on days 7–8. Cumulative mortality reached 100% on day 10. Using RNA sequencing-based transcriptome analysis after ISKNV-ZY infection, 6254 differentially expressed unigenes (DEGs) were identified, of which 3518 were upregulated and 2673 downregulated. The DEGs were associated with endocytosis, thermogenesis, oxidative phosphorylation, the JAK-STAT signaling pathway, the MAPK signaling pathway, etc. These results contribute to understanding the molecular regulation mechanism of ISKNV infection and provide a basis for ISKNV prevention.