bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan SAK ETAP Pada Koperasi Aktif di Jawa Barat Sebagai Acuan Pembuatan Aplikasi Akuntansi Koperasi Berbasis Android
Authors: Toufiq Agung Pratomo Sugito Putra  , Muhamad Ardi Nupi Hasyim
Published: March 2021

The development of accounting information technology has actually been going on for a long time. This is because the use of technology in accounting can save time and costs and the use of technology in accounting is considered very efficient. In order to provide accounting information, an information system known as the Accounting Information System (SIA) was created. A good accounting information system requires a good system design too. At  Cooperative, data processing is still done manually using Microsoft Excel, which makes this cooperative vulnerable to recording errors. Apart from errors in recording, delays in delivering information can also occur due to ineffective recording. Therefore, this cooperative needs to use a cooperative accounting information system. Which this system will later be able to assist the cooperative in carrying out the existing transaction process in the cooperative effectively and efficiently and well computerized. Based on this, this study aims to determine the financial reporting accounting information system that has been implemented by the Cooperative and to design an application-based savings and loan cooperative financial reporting accounting information system in accordance with the needs of Cooperative. This study used qualitative and experimental methods, while the application design method used was a waterfall. The system development method used in this research is Netbeans IDE with Java programming language and Mysql as database storage and iReport to design financial statements.The results obtained from the analysis and design carried out is an accounting information system design that is able to support the operational needs of Cooperative. The superiority of the resulting system is that there is a treasurer who specifically manages the finances of the Cooperative so that the process of recording financial reporting runs effectively and efficiently which has been designed according to the needs of Cooperative
Perancangan Sistem Monitoring Gangguan Akses Wifi.Id PT Telkom Wilayah Jakarta Timur Berbasis Netbeans
Authors: Mamusung Anzar Aggassi , Anshary Nico Bustanul , Sumarni Ria Asep
Published: March 2021

Abstrak— PT. Telkom Indonesia adalah yang berkerja di bidang telekomunikasi. Sistem data pembagian tiket gangguan dilakukan masih secara manual dan laporan harian teknisi masih manual. Salah satu permasalahan yang dihadapi oleh PT.Telkom Indonesia  khusus nya dibidang gangguan wifi.id yaitu masih menginput data dari teknisi untuk laporan perbaikan perharinya dan pembagian tiket masih secara manual dengan sistem cetak. Adanya resiko kesalahan dan keterlambatan informasi dalam proses penginputan dan penugasan kerja. Penelitian bertujuan untuk mendapatkan sebuah sistem  pelayanan yang nyaman dan mudah untuk diakses oleh team leader lalu, untuk mengganti sistem lama yang berada di PT Telkom Indonesia menjadi sistem baru yang sudah terkomputerisas dengan baik dan untuk mempermudah manager dan team leader dalam mengelola mengelola data gangguan wifi.id. Metode yang digunakan dalam penelitian ini adalah observasi dan grounded research. Hasil dari penelitian yaitu mendapatkan sebuah sistem pelayanan monitoring gangguan yang efisien dan dapat di akses secara mudah oleh team leader, dengan aplikasi ini juga diharapkan manager dan team leader  dapat dengan mudah mengelola data gangguan wifi.id. Kata Kunci: Perancangan Sistem, Monitoring, Gangguan Akses,Wifi.Id.
Rancang Bangun Sistem Monitoring Transformator Daya Secara Wireless Berbasis Mikrokontroler
Authors: Sandi Reza Aprita , Turahyo Turahyo , AbdulZain AbdulZain
Published: March 2021

Abstrak— Di dunia industri penggunaan transformator sebagai pengubah tegangan maupun proteksi sudah sangat banyak. Dalam pengaplikasiannya banyak terjadi kerusakan pada belitan transformator yang terjadi karena kondisi isolasi belitan yang buruk dan beban berlebih. Sehingga pengawasan kinerja transformator menjadi sangat penting. Proses monitoring secara manual dilakukan dengan membaca metering yang terpasang pada transformator. Sedangkan pada transformator tanpa metering proses monitoring dilakukan dengan mengukur secara langsung setiap parameter menggunakan peralatan ukur yang dibutuhkan. Untuk mempermudah proses monitoring tersebut perlu dibuat suatu sistem yang dapat mengukur parameter-parameter transformator secara langsung dan dari jarak jauh. Sistem monitoring transformator secara wireless menggunakan sensor tegangan, arus, dan juga temperatur yang digunakan untuk mendeteksi kondisi temperatur dari belitan transformator. Dari pengujian dapat disimpulkan bahwa hasil perancangan alat monitoring temperatur memiliki angka simpangan (error) sebesar 1.59%, tegangan 0.14%, arus 13.7%. dan daya transformator 4.4%. Pada perancangan ditambahkan perangkat proteksi transformator dengan sebuah fan pendingin yang bekerja ketika temperatur belitan melebihi 40°C dan notifikasi alarm bahaya pada saat temperatur melebihi 70°C. Hasil pengukuran parameter, dan status fan pendingin transformator dapat dibaca pada smartphone melalui koneksi wireless bluetooth hingga jangkauan maksimal 25 meter. Kata kunci: Monitoring, transformator, wireless, temperatur, mikrokontroler.
Penerapan Data Mining Untuk Memprediksi Tingkat Kemampuan Anak Dalam Mengikuti Mata Pelajaran Dengan Metode C4.5 Pada SDN 105351 Bakaran Batu
Authors: Tumanggor Aprianto , Hasugian Penda Sudiarto
Published: March 2021

The development of very advanced technology can help all aspects of life, one of which is in the world of education. The world of education certainly really needs the role of technology in managing activities by utilizing information technology. The problem that arises is the system for processing piles of data in order to know whether or not the student is able to follow the subjects at SDN 105351 Bakaran Batu. The process of implementing this data mining application only discusses predicting children's ability levels in following subjects. The data used were data from grade 5 elementary school students from the last 2 years (2018-2019) at SDN 105351 Bakaran Batu. One of the classification models is making a decision tree. By knowing the level of ability of children in following student subjects is expected to provide solutions for the future in processing student data with a computerized system, especially data regarding the level of children's ability in following subjects. The way the system works is built using the C4.5 method and data mining applications that will be designed with VB Net and Microsoft Access databases.
Sistem Pendukung Keputusan Pemilihan Topik Skripsi Program Studi Teknik Informatika Menggunakan Simple Additive Weighting (SAW)
Authors: Hulu Damayanti , Simanjorang R Mahdalena
Published: March 2021

The Department of Informatics at STMIK Pelita Nusantara is one of the departments at private universities that focuses on the science of information technology. Thesis problems in this department must also be resolved immediately, one of which is a system that utilizes information technology. It is difficult for students to determine the choice of thesis topic to be prepared as a graduation requirement. There are 7 criteria used, namely: Academic Value, Historical Project, Research Activeness, Training, Interests, Value of Practice and Research Place. Research data obtained from STMIK Pelita Nusantara applied the SAW (Simple Additive Weighting) method using Visual Basic Net 2010 with Mysql Database. From the ranking it shows that the highest result is alternative A1 where in this example the thesis topic chosen is Expert System.
Orientação Educacional: O diálogo faz a diferença
Authors: Rocha Antônio Carlos da
Published: March 2021

O presente Artigo conta com um estudo bibliográfico, somado a uma pesquisa quantitativa, além de intervenção interna e externa organizadas pelo Orientador Educacional na Escola Municipal de Ensino Fundamental Fernando de Azevedo no Município Estância Turística de Ouro Preto do Oeste – RO, ano 2019. Trata-se de um diagnóstico e intervenção feitos a partir dos desafios mais comuns que a escola enfrenta bem como a busca por alternativas viáveis para ajudar no desempenho dos alunos e no trabalho dos professores. A experiência contou com um projeto de pesquisa envolvendo teoria e prática, tendo como perspectiva final, a realização do artigo. Com o êxito no projeto, a motivação em escrever o artigo foi maior e, por essa razão, o esforço em compartilhar a experiência com os leitores. Trata-se de uma proposta útil para alunos leitores em formação no curso de pedagogia e áreas afins, a Orientadores Educacionais, mas também a toda a Equipe Gestora das escolas que pensam em um projeto inovador para a Orientação Educacional.
Mindestsicherung und Migration
Authors: Kaspar Marina
Published: March 2021

Die Bedarfsorientierte Mindestsicherung dient der verstärkten Bekämpfung und Vermeidung von Armut sowie sozialer Ausschließung. Der (dauernde) rechtmäßige Aufenthalt stellt neben der akuten Bedürftigkeit regelmäßig den Anknüpfungsmoment für einen Leistungsbezug dar. Bei der Leistungsallokation werden aber auch verfassungs-, unions- und völkerrechtliche Vorgaben berücksichtigt. Dabei stehen insbesondere Aufenthaltsrecht und sozialhilferechtliche Gleichbehandlung in einem komplexen Verhältnis zueinander. Durch die Migrations- und Fluchtbewegungen der letzten Jahre wurde die Forderung auf politischer Ebene laut, den Zugang zu Sozialhilfeleistungen für Personen ohne österreichische Staatsangehörigkeit zu beschränken. Diese Forderungen und die fehlende Einigung zwischen Bund und Ländern auf eine Reform der Mindestsicherung gipfelten schließlich in der Erlassung des Sozialhilfe-Grundsatzgesetzes. Im Lichte dieser aktuellen Entwicklungen beschäftigt sich das Werk mit Fragen eines diskriminierungsfreien Zugangs zur Mindestsicherung für nicht-österreichische Staatsangehörige.
The Use of Lafaz Amar on Ijtihad Madhhab towards the Effect of the Salat Law (Study of Ushul Fiqh)
Authors: Sajali Munawir
Published: March 2021

Lafaz amar is a study of ushul fiqh that has been explained by the scholars in their books, and lafaz amar also has various forms and types in the Qur’an and hadith, so that it will have different understandings among scholars when understanding these words of amar, however After conducting the research, based on the description and analysis carried out as explained regarding the use of lafaz amar in the Imam Madhhab's ijtihad and its effect on prayer, several conclusions can be drawn as follows: prayer there are 5 forms of lafaz amar used, namely the form of fi'il amar mufrad, fi'il amar jama, 'fi'il mudhari with lam amar, using fi'il madhi and using the word عَلَى. Whereas users of lafaz amar in the 4 books of the 4 Madhhab s of fiqh scholars, from this research there are also 5 forms of lafaz amar in general, but each Madhhab  has differences in the form of lafaz amar which is used as arguments, such as the hanafi Madhhab  in his book Hâsyiah Ibn 'Âbidîn has 4 forms of amar lafaz, namely fi'il amar mufrad, fi'il amar jama', lafaz amar fi'il madhi with lafaz كُتِبَ, أُمِرْتُ and lafaz amar using lafaz عَلَيْكُمْ, so on the Madhhab Mal Fiiki in his book Al-Kâfî Fî Fiqh Ahli Al-MadînahAl-Mâlikî, there is 1 form of lafaz amar, namely in the form of fi'il amar in jama', then the shafi'i Madhhab  in Al-Um has 5 forms of use of lafaz amar, including fi'il amar mufrad, fi'il amar jama', fi'il mudhari' with lam amar, and lafaz amar using the lafaz عَلَى, while in the book of the hambali Madhhab  in the book called Al-Mughnî there are 5 forms of use of lafaz amar in the book, including: fi'il amar mufrad, fi'il amar jama', fi'il madhi with lafaz اِفْتَرَضَ, and lafaz amar using lafaz عَلَى.
Socioeconomic factors affecting profitability of seaweed culture in Saint Martin Island of Bangladesh
Authors: Ghose TK , Hossain MI
Published: March 2021

Seaweed culture provides financial benefits and creates employment opportunity for coastal inhabitants of Bangladesh.  This study was conducted to assess profitability and to determine the socioeconomic factors that affect the profitability of seaweed culture in the coastal region. Primary data were collected from 33 seaweed farmers for the study. The data were collected during the period of March 15 to March 31, 2018 through direct interviews and observation using a semi structured questionnaire and a check list. Both tabular and functional analyses were used to achieve the objectives of the study. It is evident from the study that seaweed culture is a profitable business. The total per square-meter cost of seaweed production was Tk. 1520 with a gross return of Tk. 2801.4 and net return was estimated at Tk. 1281.4 with a Benefit-Cost Ratio (BCR) 1.82. To estimate the contribution of different inputs on seaweed production through Cobb-Douglas production function, the explanatory variables considered were: bamboo cost, rope cost, human labour cost, repairing cost, age, experience, training, education and number of family members. Among these variables, labour and training were found positively significant at 1 percent level of significance with the regression coefficient of 0.209 and 0.556, respectively. This indicates that seaweed culture might be brought economic benefits of the community if they received more training and employ more labour. Therefore, policy should be focused on creating appropriate training arrangement and skilled labour to the coastal community for getting higher benefit. Seaweed farmers think that seaweed culture can be adopted as an alternative livelihood option if the government and other stakeholders help them with necessary supports for seaweed culture.

Progressive Agriculture 31 (3): 227-234, 2020
A distinct molecular signature on anhydrobiotic cyanobacterial metallothioneins
Authors: Contiliani Danyel Fernandes , Moraes Vitor Nolasco de , Ribeiro Yasmin de Araújo , Pereira Tiago Campos
Published: March 2021

Anhydrobiosis refers to a state of suspended animation in which some organisms enter when exposed to extreme desiccation, ensuring them an outstanding tolerance to several physical stresses due to molecular and cellular adaptations. Metallothioneins (MTs) are short cysteine-rich metal-chelating proteins that work as a cellular protection element in metal ion-rich conditions. Here we aimed to investigate possible molecular signatures in primary and tertiary structures in anhydrobiotic cyanobacterial MTs. Anhydrobiotic and non-anhydrobiotic cyanobacterial MT amino acid sequences were retrieved from NCBI database and aligned in Clustal Omega server. Additionally, the amino acid compositions of these sequences were determined by GeneRunner. Further, we carried out homology-modeling via SWISS-MODEL, structural superposition in UCSF Chimera 1.4 Matchmaker tool and ligand-binding site prediction via COFACTOR. In silico analyses revealed specific divergences in amino acid positions between MT groups, evidencing positive and negative selections, however without affecting final protein structures. Some of these changes on polypeptide sequence potentially enhance protein stabilization during desiccation, whereas others possibly act as additional metal-ion coordinating residues. Analyses on the molecular adaptations on anhydrobiotic cyanobacterial MTs help shed light on their molecular functions and biological roles, as well as may have applications on the development of desiccation- and metal-tolerant organisms.
Câncer de pênis e fatores associados a metástases em linfonodos inguinais
Authors: Santos Kelly Monteiro dos , Barros Felipe Dubourcq de , Araujo-Mariz Carolline de
Published: March 2021

Objetivo: O câncer de pênis tem alta incidência em alguns países em desenvolvimento e a metástase em linfonodos da cadeia inguinal é um grande desafio no tratamento dessa neoplasia. O objetivo deste estudo foi identificar a prevalência de metástases em linfonodos inguinais e seus fatores associados em pacientes com câncer de pênis.Métodos: Conduzimos uma análise retrospectiva de todas as consultas de lesões penianas realizadas por homens adultos em nossa instituição. Metástase em linfonodos inguinais constituiu o evento de interesse e foi definida a partir da presença de carcinoma escamocelular em linfonodos inguinais, confirmado por exame anatomopatológico. Foi realizada análise de regressão logística bivariada e a magnitude das associações expressa pelo Odds Ratio como estimativa do risco relativo com intervalo de confiança de 90%. Resultados: A prevalência de metástases observada entre os 179 casos de carcinoma escamocelular de pênis foi de 24%. A presença de linfonodos palpáveis na região inguinal no momento do diagnóstico (p = 0,008) e o uso abusivo de álcool (p = 0,07) foram fatores associados independentes para que a doença se tornasse mais agressiva.Conclusões: Pacientes com diagnóstico de carcinoma epidermóide de pênis, que apresentam linfonodos palpáveis na região inguinal durante o exame clínico e aqueles que fazem uso de bebidas alcoólicas, devem ser alvo precoce de intervenções nos serviços de urooncologia.
Evolução da Lei de Responsabilidade Fiscal: Uma análise nos municípios da região sul do Brasil
Authors: Maciel Ana Maria Heinrichs , Miguel Lisiane de , Beló Alexsander , Pinto Nelson Guilherme Machado
Published: March 2021

No Brasil, e especificamente na Região Sul, discute-se como encontrar alternativas para conseguir adequar os gastos aquilo que de fato foi planejado. Neste contexto, a Lei de Responsabilidade Fiscal (LRF) vem a contribuir para um maior controle e responsabilidade dos gastos públicos. Desta forma, o objetivo deste estudo é fazer uma análise dos municípios dos três estados da Região Sul do Brasil quanto a sua evolução em relação a LRF e as receitas e despesas no período compreendido entre 2011 e 2018. O método de pesquisa utilizado para elaboração do artigo é de natureza descritiva, quantitativa e documental. Conclui-se que no decorrer do tempo analisado, houve grande evolução dos municípios com a intenção de adequarem-se as imposições da lei, no entanto, há dificuldade dos pequenos municípios em manter o equilíbrio nas contas públicas. Assim, de forma ampla, os municípios catarinenses demonstraram que possuem grandes recorrências e dificuldades no fechamento dos exercícios devido as grandes despesas, e também quanto aos limites com gastos de pessoal, que junto com os municípios paranaenses representam a elevado número descumprimento do limite. Desta forma, o estado gaúcho apresentou os melhores índices do estudo, considerando a situação dos municípios, bem como uma análise temporal evolutiva.
Aspectos éticos e trâmites da publicação de relato de caso no Brasil
Authors: Cândido Estelita Lima , Ribeiro Tatianne Régia Gomes , Brito Maria Auxiliadora Ferreira , Rodrigues Sandhara Ribeiro , Feitosa Maria Darcylene de Souza , Feitosa Francisco Pereira Júnior , Freitas Jucycler Ferreira
Published: March 2021

A publicação de um relato de caso tem por objetivo transmitir aos outros uma informação nova, relevante, geralmente conduzida por um caminho inabitual. Ele pode gerar um potencial para modificar a prática clínica, ou hipóteses para novos estudos. Mesmo não se tratando de uma pesquisa, é necessário que todos os princípios éticos que visem à proteção do participante sejam respeitados. O presente artigo apresenta as diretrizes para a redação de relatos de caso de acordo com as resoluções e normativas vigentes no Brasil, bem como a tramitação dos mesmos no sistema Comitês de Ética em Pesquisa/Comissão Nacional de Ética em Pesquisa. Tais informações são oportunas na medida em que norteiam pesquisadores e Comitês de ética em pesquisas com seres humanos.
PENERAPAN PERMAINAN EDUKATIF MEDIA BERGAMBAR
Authors: Setiyowati Nunuk
Published: March 2021

Play is the world of children. In play there is pleasure and satisfaction without coercion oriented to the process rather than the end result. Piaget (1962), suggested that learning while playing and playing while learning is an activity that must be presented so that children have readiness to enter further education. Educative games of pictorial media have an important role in developing all aspects of children’s intelligence, because in educational play activities, pictorial games of children become active in moving and thinking so that new ideas and experiences that will be important for their growth and development will emerge. Group A children are one of the times when children have to get a lot of positive stimulation in learning to develop their full potential, imagination and intelligence aspects so that they develop optimally. At this time it is often called a critical period or a golden age that whill not be repeated again. So that the role of parent, teacher and the environment becomes the main pillar for children’s growht and development, so that children grow intro tough generations who excel and are ready to face every challenge and all the changing times.
Effects of adenosine A1 receptor antagonism on lipogenesis and lipolysis in isolated rat adipocytes
Authors: Szkudelski T , Szkudelska K , Nogowski L
Published: March 2021

Adenosine is secreted from adipocytes, binds to adenosine A1
receptor and modulates various functions of these cells. In the
present study, the effects of an adenosine A1 receptor antagonist
(DPCPX; 0.01, 0.1 and 1 μM) on lipogenesis, glucose transport,
lipolysis and the antilipolytic action of insulin were tested in rat
adipocytes. DPCPX had a very weak effect on lipogenesis and did
not significantly affect glucose uptake. In adipocytes incubated
with 1 μM DPCPX, lipolysis increased. This effect was blunted by
insulin and by a direct inhibitor of protein kinase A. Moreover,
0.1 μM DPCPX substantially enhanced the lipolytic response to
epinephrine and increased cAMP in adipocytes. However, DPCPX
was ineffective when lipolysis was stimulated by direct activation
of protein kinase A. Adipocyte exposure to epinephrine and
insulin with or without 0.1 μM DPCPX demonstrated that this
antagonist increased the release of glycerol. However, despite
the presence of DPCPX, insulin was able to reduce lipolysis. It is
concluded that DPCPX had a weak effect on lipogenesis, whereas
lipolysis was significantly affected. The partial antagonism of
adenosine A1 receptor increased lipolysis in cells incubated with
epinephrine alone and epinephrine with insulin due to the
synergistic action of 0.1 μM DPCPX and epinephrine.
Plasma prohepcidin as a negative acute phase reactant after large cardiac surgery with a deep hypothermic circulatory arrest
Authors: Maruna P , Lindner J , Kunštýř J , Kubzová K , Hubáček J
Published: March 2021

Hepcidin is a key regulator of iron metabolism and a mediator of
anemia in inflammation. Recent in vitro studies recognized
prohepcidin as a type II acute phase protein regulating via
interleukin-6. The aim of the present study was to investigate the
time course of plasma prohepcidin after a large cardiac surgery in
relation to IL-6 and other inflammatory parameters. Patients with
chronic thromboembolic hypertension (n=22, males/females
14/8, age 51.9±10.2 years) underwent pulmonary
endarterectomy using cardiopulmonary bypass and deep
hypothermic circulatory arrest were included into study. Arterial
concentrations of prohepcidin, IL-1β, IL-6, IL-8, tumor necrosis
factor-α, and C-reactive protein were measured before/after
sternotomy, after circulatory arrest, after separation from bypass,
and then 12, 18, 24, 36, 48 h and 72 h after the separation from
bypass. Hemodynamic parameters, hematocrit and markers of
iron metabolism were followed up. Pulmonary endarterectomy
induced a 48 % fall in plasma prohepcidin; minimal
concentrations were detected after separation from
cardiopulmonary bypass. Prohepcidin decline correlated with an
extracorporeal circulation time (p<0.01), while elevated IL-6
levels were inversely associated with duration of prohepcidin
decline. Postoperative prohepcidin did not correlate with markers
of iron metabolism or hemoglobin concentrations within a 72-h
period after separation from CPB. Prohepcidin showed itself as a
negative acute phase reactant during systemic inflammatory
response syndrome associated with a cardiac surgery. Results
indicate that the evolution of prohepcidin in postoperative period
implies the antagonism of stimulatory effect of IL-6 and
contraregulatory factors inhibiting prohepcidin synthesis or
increasing prohepcidin clearance.
Effect of chronic nifedipine treatment on blood pressure and adrenergic responses of isolated mesenteric artery in young rats with developing spontaneous hypertension
Authors: Zemančíková A , Török J
Published: March 2021

It is documented that in chronic hypertensive state there is an
increased vasodepressor response to calcium channel antagonists
such as the dihydropyridine derivate nifedipine. This effect is
generally proportional to initial blood pressure as was
demonstrated in several models of experimental hypertension. In
the present study we investigated the effect of chronic nifedipine
treatment on the development of cardiovascular system in young
spontaneously hypertensive rats (SHR) in order to evaluate
whether it could prevent the abnormalities leading to
hypertensive state. Four- and eight-week-old rats were treated
with nifedipine (50 mg/kg/day) for 4 weeks. Blood pressure of
nifedipine-treated SHR remained at the initial level in contrast to
their untreated controls where it continued to increase. In both
age groups, chronic nifedipine administration reduced neurogenic
contractions of isolated superior mesenteric artery, but did not
significantly affect the dose-response curve to exogenous
noradrenaline in 8-week-old rats. In contrast, maximum response
to noradrenaline was significantly attenuated in mesenteric artery
of 12-week-old nifedipine-treated SHR. We can presume that the
antihypertensive effect of nifedipine is similar in both stages of
spontaneous hypertension development, but the mechanisms
involved might be different. It seems that chronic reduction of
calcium influx during the rapid phase of pathological blood
pressure increase in SHR may eliminate the effect of enhanced
sympathetic tone, which may have unfavorable consequences on
cardiovascular structure and function.
WAYANG GODHONG SEBAGAI MEDIA EDUKASI CINTA BUDAYA DAN ALAM SEJAK USIA DINI
Authors: Purwanto Agus , Octavia Ercilia Rini , Adi Sigit Purnomo
Published: March 2021

AbstrakSeiring dengan perkembangan zaman, pertunjukan wayang berfungsi menjadi media pembelajaran. Demikian halnya dengan Wayang Godhong.  Wayang kontemporer yang berasal dari godhong (daun) jati, cengkeh, dan kopi ini mencoba menjadikan pertunjukan wayangnya sebagai media edukasi cinta budaya dan alam sejak usia dini di Kabupaten Magelang. Artikel yang disusun melalui metode penelitian kualitatif deskriptif ini, berhasil menemukan visualisasi figur/karakter Wayang Godhong yang representatif untuk anak usia dini dengan pendekatan visual yang ramah dan lucu. Kemudian proses penyampaian dalang Wayang Godhong saat pentas yang harus menggunakan mimik jelas (karena dalang di depan anak-anak, tidak di balik layar), sehingga bahasa yang digunakan pun harus bahasa yang mudah dipahami anak-anak dengan penyampaian humorisme.  Setelah melihat pementasan, anak-anak pun berkeinginan melakukan seperti yang dipesankan dalam pementasan yakni menanam pohon sebagai upaya melestarikan alam ini serta menjadi suka/cinta akan wayang. Dengan demikian Wayang Godhong dianggap mampu menjadi media edukasi cinta wayang sebagai hasil dari budaya Indonesia dan cinta alam dengan menanam/merawat pohon sejak usia dini. Kata Kunci: budaya dan alam, media edukasi, anak usia dini, Wayang Godhong AbstractAlong with the times, puppet shows function as learning media. Like wise with Wayang Godhong. Wayang Godhong, a form of contemporary wayang made from leaves of teak, cloves, and coffee, attempts to become a medium for children to foster a sense of love for nature and culture, especially in the Magelang Regency. This article, compiled through descriptive qualitative research methods, has succeeded in finding the visualization of the representative Wayang Godhong figures/characters for early childhood with a friendly and funny visual approach. The performance process of storytelling by the puppeteer must be used a clear facial expression (because the puppeteer stays in front of the children, not behind the scenes), the language must be easy to understand by the children by conveying humorism. After seeing the performance, the children wanted to do as instructed in the wayang performance, such as planting trees as an effort to preserve nature and became interested in wayang. Thereby, Wayang Godhong is considered capable of being a love for wayang educational media as a result of Indonesian culture and love for nature by planting/caring for trees from an early age. Keywords: children, culture and nature, education media, Godhong puppet
Effects of Shear Stress on Production of FVIII and vWF in a Cell-Based Therapeutic for Hemophilia A
Authors: Trevisan Brady , Morsi Alshaimaa , Aleman Julio , Rodriguez Martin , Shields Jordan , Meares Diane , Farland Andrew M. , Doering Christopher B. , Spencer H. Trent , Atala Anthony , Skardal Aleks , Porada Christopher D. , Almeida-Porada Graça
Published: March 2021

Microfluidic technology enables recapitulation of organ-level physiology to answer pertinent questions regarding biological systems that otherwise would remain unanswered. We have previously reported on the development of a novel product consisting of human placental cells (PLC) engineered to overexpress a therapeutic factor VIII (FVIII) transgene, mcoET3 (PLC-mcoET3), to treat Hemophilia A (HA). Here, microfluidic devices were manufactured to model the physiological shear stress in liver sinusoids, where infused PLC-mcoET3 are thought to lodge after administration, to help us predict the therapeutic outcome of this novel biological strategy. In addition to the therapeutic transgene, PLC-mcoET3 also constitutively produce endogenous FVIII and von Willebrand factor (vWF), which plays a critical role in FVIII function, immunogenicity, stability, and clearance. While vWF is known to respond to flow by changing conformation, whether and how shear stress affects the production and secretion of vWF and FVIII has not been explored. We demonstrated that exposure of PLC-mcoET3 to physiological levels of shear stress present within the liver sinusoids significantly reduced mRNA levels and secreted FVIII and vWF when compared to static conditions. In contrast, mRNA for the vector-encoded mcoET3 was unaltered by flow. To determine the mechanism responsible for the observed decrease in FVIII and vWF mRNA, PCR arrays were performed to evaluate expression of genes involved in shear mechanosensing pathways. We found that flow conditions led to a significant increase in KLF2, which induces miRNAs that negatively regulate expression of FVIII and vWF, providing a mechanistic explanation for the reduced expression of these proteins in PLC under conditions of flow. In conclusion, microfluidic technology allowed us to unmask novel pathways by which endogenous FVIII and vWF are affected by shear stress, while demonstrating that expression of the therapeutic mcoET3 gene will be maintained in the gene-modified PLCs upon transplantation, irrespective of whether they engraft within sites that expose them to conditions of shear stress.
Adaptive Mechanisms of Tumor Therapy Resistance Driven by Tumor Microenvironment
Authors: Wu Peijie , Gao Wei , Su Miao , Nice Edouard C. , Zhang Wenhui , Lin Jie , Xie Na
Published: March 2021

Cancer is a disease which frequently has a poor prognosis. Although multiple therapeutic strategies have been developed for various cancers, including chemotherapy, radiotherapy, and immunotherapy, resistance to these treatments frequently impedes the clinical outcomes. Besides the active resistance driven by genetic and epigenetic alterations in tumor cells, the tumor microenvironment (TME) has also been reported to be a crucial regulator in tumorigenesis, progression, and resistance. Here, we propose that the adaptive mechanisms of tumor resistance are closely connected with the TME rather than depending on non-cell-autonomous changes in response to clinical treatment. Although the comprehensive understanding of adaptive mechanisms driven by the TME need further investigation to fully elucidate the mechanisms of tumor therapeutic resistance, many clinical treatments targeting the TME have been successful. In this review, we report on recent advances concerning the molecular events and important factors involved in the TME, particularly focusing on the contributions of the TME to adaptive resistance, and provide insights into potential therapeutic methods or translational medicine targeting the TME to overcome resistance to therapy in clinical treatment.
Factors Associated With the Microbiome in Moderate–Late Preterm Babies: A Cohort Study From the DIAMOND Randomized Controlled Trial
Authors: Chong Clara Yieh Lin , Vatanen Tommi , Alexander Tanith , Bloomfield Frank H. , O’Sullivan Justin M.
Published: March 2021

The gut microbiota of preterm infants is affected by perinatal factors and, in turn, may impact upon infant health. In this study, we collected fecal samples at Day-10 (D10) and 4-months corrected-age (4M) from 227 moderate–late preterm (MLPT) babies enrolled in a randomized controlled trial of nutritional management. A total of 320 samples underwent 16S amplicon sequencing, and shotgun metagenomic sequencing was performed on 94 samples from the 4M time point. The microbiome of babies whose families lived in lower socioeconomic status (SES) areas exhibited a significantly higher microbial alpha diversity at D10 (Wilcoxon test, p = 0.021), greater abundance of Bifidobacterium (linear model, q = 0.020) at D10 and Megasphaera (q = 0.031) at 4M. Hospital of birth explained 5.2% of the observed variance in 4M samples (PERMANOVA, p = 0.038), with Staphylococcus aureus more abundant in fecal samples from babies born in Middlemore hospital (linear model, q = 0.016). Maternal antibiotic (Wilcoxon test, p = 0.013) and probiotic (p = 0.04) usage within the four-week period before sample collection was associated with a reduction in the alpha diversity of D10 samples. Infant probiotic intake explained 2.1% (PERMANOVA, p = 0.021) of the variance in the D10 microbial profile with increased Lactobacillus (linear model, q = 1.1 × 10−10) levels. At 4M, the microbiome of infants who were breastmilk fed had reduced alpha diversity when compared to non-breastmilk fed infants (Wilcoxon test, p < 0.05). Although causality cannot be inferred within our study, we conclude that in MLPT babies, maternal socioeconomic factors, as well as the perinatal medical environment and nutrition impact on the development of the newborn microbiome.
Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection
Authors: Bismuth Hanna D. , Brasseur Gaël , Ezraty Benjamin , Aussel Laurent
Published: March 2021

Over the last decade, an increasing number of reports presented Galleria mellonella larvae as an important model to study host-pathogen interactions. Coherently, increasing information became available about molecular mechanisms used by this host to cope with microbial infections but few of them dealt with oxidative stress. In this work, we addressed the role of reactive oxygen species (ROS) produced by the immune system of G. mellonella to resist against Salmonella enterica, an intracellular pathogen responsible for a wide range of infections. We confirmed that Salmonella was pathogen for G. mellonella and showed that it had to reach a minimal bacterial load within the hemolymph to kill the larvae. ROS production by G. mellonella was revealed by the virulence defects of Salmonella mutants lacking catalases/peroxiredoxins or cytoplasmic superoxide dismutases, both strains being highly sensitive to these oxidants. Finally, we used bacterial transcriptional fusions to demonstrate that hydrogen peroxide (H2O2) was produced in the hemolymph of Galleria during infection and sensed by S. enterica. In line with this observation, the H2O2-dependent regulator OxyR was found to be required for bacterial virulence in the larvae. These results led us to conclude that ROS production is an important mechanism used by G. mellonella to counteract bacterial infections and validate this host as a relevant model to study host-pathogen interactions.
Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy
Authors: Schuldt Maike , Dorsch Larissa M. , Knol Jaco C. , Pham Thang V. , Schelfhorst Tim , Piersma Sander R. , dos Remedios Cris , Michels Michelle , Jimenez Connie R. , Kuster Diederik W. D. , van der Velden Jolanda
Published: March 2021

Background: Sex-differences in clinical presentation contribute to the phenotypic heterogeneity of hypertrophic cardiomyopathy (HCM) patients. While disease prevalence is higher in men, women present with more severe diastolic dysfunction and worse survival. Until today, little is known about the cellular differences underlying sex-differences in clinical presentation.

Methods: To define sex-differences at the protein level, we performed a proteomic analysis in cardiac tissue obtained during myectomy surgery to relieve left ventricular outflow tract obstruction of age-matched female and male HCM patients harboring a sarcomere mutation (n = 13 in both groups). Furthermore, these samples were compared to 8 non-failing controls. Women presented with more severe diastolic dysfunction.

Results: Out of 2099 quantified proteins, direct comparison of male, and female HCM samples revealed only 46 significantly differentially expressed proteins. Increased levels of tubulin and heat shock proteins were observed in female compared to male HCM patients. Western blot analyses confirmed higher levels of tubulin in female HCM samples. In addition, proteins involved in carbohydrate metabolism were significantly lower in female compared to male samples. Furthermore, we found lower levels of translational proteins specifically in male HCM samples. The disease-specificity of these changes were confirmed by a second analysis in which we compared female and male samples separately to non-failing control samples. Transcription factor analysis showed that sex hormone-dependent transcription factors may contribute to differential protein expression, but do not explain the majority of protein changes observed between male and female HCM samples.

Conclusion: In conclusion, based on our proteomics analyses we propose that increased levels of tubulin partly underlie more severe diastolic dysfunction in women compared to men. Since heat shock proteins have cardioprotective effects, elevated levels of heat shock proteins in females may contribute to later disease onset in woman, while reduced protein turnover in men may lead to the accumulation of damaged proteins which in turn affects proper cellular function.
Muscle-Bone Crosstalk in the Masticatory System: From Biomechanical to Molecular Interactions
Authors: Buvinic Sonja , Balanta-Melo Julián , Kupczik Kornelius , Vásquez Walter , Beato Carolina , Toro-Ibacache Viviana
Published: March 2021

The masticatory system is a complex and highly organized group of structures, including craniofacial bones (maxillae and mandible), muscles, teeth, joints, and neurovascular elements. While the musculoskeletal structures of the head and neck are known to have a different embryonic origin, morphology, biomechanical demands, and biochemical characteristics than the trunk and limbs, their particular molecular basis and cell biology have been much less explored. In the last decade, the concept of muscle-bone crosstalk has emerged, comprising both the loads generated during muscle contraction and a biochemical component through soluble molecules. Bone cells embedded in the mineralized tissue respond to the biomechanical input by releasing molecular factors that impact the homeostasis of the attaching skeletal muscle. In the same way, muscle-derived factors act as soluble signals that modulate the remodeling process of the underlying bones. This concept of muscle-bone crosstalk at a molecular level is particularly interesting in the mandible, due to its tight anatomical relationship with one of the biggest and strongest masticatory muscles, the masseter. However, despite the close physical and physiological interaction of both tissues for proper functioning, this topic has been poorly addressed. Here we present one of the most detailed reviews of the literature to date regarding the biomechanical and biochemical interaction between muscles and bones of the masticatory system, both during development and in physiological or pathological remodeling processes. Evidence related to how masticatory function shapes the craniofacial bones is discussed, and a proposal presented that the masticatory muscles and craniofacial bones serve as secretory tissues. We furthermore discuss our current findings of myokines-release from masseter muscle in physiological conditions, during functional adaptation or pathology, and their putative role as bone-modulators in the craniofacial system. Finally, we address the physiological implications of the crosstalk between muscles and bones in the masticatory system, analyzing pathologies or clinical procedures in which the alteration of one of them affects the homeostasis of the other. Unveiling the mechanisms of muscle-bone crosstalk in the masticatory system opens broad possibilities for understanding and treating temporomandibular disorders, which severely impair the quality of life, with a high cost for diagnosis and management.
The Effectiveness of Image Augmentation in Deep Learning Networks for Detecting COVID-19: A Geometric Transformation Perspective
Authors: Elgendi Mohamed , Nasir Muhammad Umer , Tang Qunfeng , Smith David , Grenier John-Paul , Batte Catherine , Spieler Bradley , Leslie William Donald , Menon Carlo , Fletcher Richard Ribbon , Howard Newton , Ward Rabab , Parker William , Nicolaou Savvas
Published: March 2021

Chest X-ray imaging technology used for the early detection and screening of COVID-19 pneumonia is both accessible worldwide and affordable compared to other non-invasive technologies. Additionally, deep learning methods have recently shown remarkable results in detecting COVID-19 on chest X-rays, making it a promising screening technology for COVID-19. Deep learning relies on a large amount of data to avoid overfitting. While overfitting can result in perfect modeling on the original training dataset, on a new testing dataset it can fail to achieve high accuracy. In the image processing field, an image augmentation step (i.e., adding more training data) is often used to reduce overfitting on the training dataset, and improve prediction accuracy on the testing dataset. In this paper, we examined the impact of geometric augmentations as implemented in several recent publications for detecting COVID-19. We compared the performance of 17 deep learning algorithms with and without different geometric augmentations. We empirically examined the influence of augmentation with respect to detection accuracy, dataset diversity, augmentation methodology, and network size. Contrary to expectation, our results show that the removal of recently used geometrical augmentation steps actually improved the Matthews correlation coefficient (MCC) of 17 models. The MCC without augmentation (MCC = 0.51) outperformed four recent geometrical augmentations (MCC = 0.47 for Data Augmentation 1, MCC = 0.44 for Data Augmentation 2, MCC = 0.48 for Data Augmentation 3, and MCC = 0.49 for Data Augmentation 4). When we retrained a recently published deep learning without augmentation on the same dataset, the detection accuracy significantly increased, with aχMcNemar′s statistic2=163.2and ap-value of 2.23 × 10−37. This is an interesting finding that may improve current deep learning algorithms using geometrical augmentations for detecting COVID-19. We also provide clinical perspectives on geometric augmentation to consider regarding the development of a robust COVID-19 X-ray-based detector.
Fluorometric Liposome Screen for Inhibitors of a Physiologically Important Bacterial Ion Channel
Authors: Fernandes Andreia S. , Pombinho António , Teixeira-Duarte Celso M. , Morais-Cabral João H. , Harley Carol A.
Published: March 2021

The bacterial K+ homeostasis machinery is widely conserved across bacterial species, and different from that in animals. Dysfunction in components of the machinery has an impact on intracellular turgor, membrane potential, adaptation to changes in both extracellular pH and osmolarity, and in virulence. Using a fluorescence-based liposome flux assay, we have performed a high-throughput screen to identify novel inhibitors of the KtrAB ion channel complex from Bacillus subtilis, a component of the K+ homeostasis machinery that is also present in many bacterial pathogens. The screen identified 41 compounds that inhibited K+ flux and that clustered into eight chemical groups. Many of the identified inhibitors were found to target KtrAB with an in vitro potency in the low μM range. We investigated the mechanisms of inhibition and found that most molecules affected either the membrane component of the channel, KtrB alone or the full KtrAB complex without a preference for the functional conformation of the channel, thus broadening their inhibitory action. A urea derivative molecule that inhibited the membrane component of KtrAB affected cell viability in conditions in which KtrAB activity is essential. With this proof-of-concept study, we demonstrate that targeting components of the K+ homeostasis machinery has the potential as a new antibacterial strategy and that the fluorescence-based flux assay is a robust tool for screening chemical libraries.