bluuebunny/crossref_metadata_2025 huggingface dataset:
Healthcare-associated urinary tract infections with onset post hospital discharge
Authors: Elman Miriam R. , Williams Craig D. , Bearden David T. , Townes John M. , Heintzman John D. , Lapidus Jodi A. , Kullar Ravina , Markwardt Sheila , Trieu Amanda T. , Vahidi Arrash A. , McGregor Jessina C.
Published: June 2019

Objective:

Current surveillance for healthcare-associated (HA) urinary tract infection (UTI) is focused on catheter-associated infection with hospital onset (HO-CAUTI), yet this surveillance does not represent the full burden of HA-UTI to patients. Our objective was to measure the incidence of potentially HA, community-onset (CO) UTI in a retrospective cohort of hospitalized patients.

Design:

Retrospective cohort study.

Setting:

Academic, quaternary care, referral center.

Patients:

Hospitalized adults at risk for HA-UTI from May 2009 to December 2011 were included.

Methods:

Patients who did not experience a UTI during the index hospitalization were followed for 30 days post discharge to identify cases of potentially HA-CO UTI.

Results:

We identified 3,273 patients at risk for potentially HA-CO UTI. The incidence of HA-CO UTI in the 30 days post discharge was 29.8 per 1,000 patients. Independent risk factors of HA-CO UTI included paraplegia or quadriplegia (adjusted odds ratio [aOR], 4.6; 95% confidence interval [CI], 1.2–18.0), indwelling catheter during index hospitalization (aOR, 1.5; 95% CI, 1.0–2.3), prior piperacillin-tazobactam prescription (aOR, 2.3; 95% CI, 1.1–4.5), prior penicillin class prescription (aOR, 1.7; 95% CI, 1.0–2.8), and private insurance (aOR, 0.6; 95% CI, 0.4–0.9).

Conclusions:

HA-CO UTI may be common within 30 days following hospital discharge. These data suggest that surveillance efforts may need to be expanded to capture the full burden to patients and better inform antibiotic prescribing decisions for patients with a history of hospitalization.
The limited politicization of European integration in Italy: lacking issue clarity and weak voter responses
Authors: Carrieri Luca
Published: June 2019

This article analyzes the politicization of European integration in Italy. Has the euro crisis catalyzed a new electoral supply, which may have been matched by voters’ responses? After the crisis, Italian parties have increased their entrepreneurial efforts to prime EU issues into the political debate, trying to win over votes. This pattern may have led to a full politicization of European integration. To study the transformations in party strategies and voting behavior, I analyze the fluctuations in EU issue entrepreneurship and EU issue voting between 2009 and 2014. The findings reveal mixed results: parties have actually developed new strategies on EU issues, but without significantly changing voter preferences along the pro/anti-European dimension.
Marangoni circulation by UV light modulation on sessile drop for particle agglomeration
Authors: Li Tianyi , Kar Aravinda , Kumar Ranganathan
Published: June 2019

An analytical solution of a biharmonic equation is presented in axisymmetric toroidal coordinates for Stokes flow due to surface tension gradient on the free surface of sessile drops. The stream function profiles exhibit clockwise and counter-clockwise toroidal volumes. The ring or dot formed by the downward dividing streamlines between these volumes predicts the experimentally deposited particle ring or dot well. This finding suggests that the downward dividing streamline can be taken to be a reasonable indicator of where deposition occurs. Different light patterns directed at different locations of the droplet can give rise to a single spot or ring. A relationship between the positions of the light intensity peak and possible locations of particle deposition is analysed to demonstrate that the streamlines can be generated on-demand to achieve particle deposition at predetermined locations on the substrate. Toroidal corner vortices called Moffatt eddies have appeared in other corner flows and develop in this optical Marangoni flow as well near the contact line.
Commentary: Gold Coast 2018
Authors: Powell Michael
Published: June 2019

Commentary
The Gold Coast 2018 Commonwealth Games
Authors: Powell Michael
Published: June 2019

On 4 April 2018, some 35,000 people jammed into Carrara Stadium on Queensland’s Gold Coast to see athletes from seventy-one nations march in to the Opening Ceremony of the 21st Commonwealth Games. It was a beautiful Gold Coast day and signalled the start to eleven days of competition across venues from Cairns in the north of Queensland to Coolangatta on the southern border with New South Wales. It was also the culmination of a ten-year journey that had started back in 2008 when Premier Anna Bligh mooted the suggestion of bringing the Games to the Gold Coast as a means of reviving and transforming a Gold Coast economy that had been badly affected by the Global Financial Crisis.
Barry Shaw (ed.), 'Brisbane at War 1899–1918', Brisbane: Boolarong Press and the Brisbane History Group, 2018, 242 pp., ISBN 9 7819 2552 2648, A$34.99
Authors: Crotty Martin
Published: June 2019

Barry Shaw (ed.), Brisbane at War 1899–1918, Brisbane: Boolarong Press and the Brisbane History Group, 2018, 242 pp., ISBN 9 7819 2552 2648, A$34.99.
Investigating death in Moreton Bay
Authors: Butterworth Lee
Published: June 2019

English common law was applied in the New South Wales penal colony when it was founded by Governor Arthur Phillip in 1788. Phillip’s second commission granted him sole authority to appoint coroners and justices of the peace within the colony. The first paid city coroner was appointed in 1810 and only five coroners served the expanding territory of New South Wales by 1821. To relieve the burden on coroners, justices of the peace were authorised to conduct magisterial inquiries as an alternative to inquests. When the Moreton Bay settlement was established, and land was opened up to free settlers, justices were relocated from New South Wales to the far northern colony. Nonetheless, the administration of justice, along with the function of the coroner, was hindered by issues of isolation, geography and poor administration by a government far removed from the evolving settlement. This article is about death investigation and the role of the coroner in Moreton Bay. By examining a number of case studies, it looks at the constraints faced by coroners, deaths due to interracial violence and deaths not investigated. It concludes that not all violent and unexplained deaths were investigated in accordance with coronial law due to a paucity of legally qualified magistrates, the physical limitations of local conditions and the denial of justice to Aborigines as subjects of the Crown.
Reconstructing the Battle of 'Narawai (Moongalba)
Authors: Kerkhove Ray
Published: June 2019

The Battle of ’Narawai on North Stradbroke Island, and skirmishes that culminated in this event (c. 1827–32) have been sidelined in recent decades, based on the assumption that the event was more likely a massacre, and that sources are too conflicted to build a workable narrative. Here we utilise known and unexamined sources, and the untapped oral tradition and environmental knowledge of Stradbroke Island Aboriginal peoples, to reconstruct both the build-up and phases of the confrontation. We find that our primary sources for this incident ultimately derive from Aboriginal informants; together with current Aboriginal perspectives, these allow a more nuanced and Aboriginal-driven narrative than is normally possible for a frontier wars skirmish. It is argued that the Battle of ’Narawai was not a one-sided massacre but rather a well-planned operation by Aboriginal combatants, orchestrated to provide tactical advantages. We contend that the battle merged tactics of traditional pullen-pullen (inter-tribal tournaments) with strategies more suited to the demands of the frontier wars, and that it was perceived as a victory by Aboriginal Stradbroke Islanders.
Avoiding the white elephants
Authors: Farndon David , Burton Paul
Published: June 2019

The Gold Coast 2018 Commonwealth Games presented the host city with a number of opportunities to improve its infrastructure and sporting facilities in line with its long-term strategic vision to transition to being a more mature and sustainable Australian city. However, major events such as the Commonwealth Games have a chequered history of bestowing lasting benefits and a positive legacy on the host city. This article examines the ways in which infrastructure planning for the 2018 Games was used to underpin the success experienced by the Gold Coast in harnessing the event to achieve broader city building objectives. It also reflects critically on how major event-led development can be used to support existing strategic city plans.
The effects of antibiotics and melatonin on hepato-intestinal inflammation and gut microbial dysbiosis induced by a short-term high-fat diet consumption in rats
Authors: Yildirim Alper , Arabacı Tamer Sevil , Sahin Duran , Bagriacik Fatma , Kahraman Merve M. , Onur Nilsu D. , Cayirli Yusuf B. , Cilingir Kaya Özlem T. , Aksu Burak , Akdeniz Esra , Yuksel Meral , Çetinel Şule , Yeğen Berrak Ç.
Published: June 2019

High-fat diet (HFD) consumption leads to metabolic disorders, gastrointestinal dysfunction and intestinal dysbiosis. Antibiotics also disrupt the composition of intestinal microbiota. The aim of the present study was to investigate the impact of a short-term feeding with HFD on oxidative status, enteric microbiota, intestinal motility and the effects of antibiotics and/or melatonin treatments on diet-induced hepato-intestinal dysfunction and inflammation. Male Sprague–Dawley rats were pair-fed with either standard chow or HFD (45 % fat) and were given tap water or melatonin (4 mg/kg per d) or melatonin plus antibiotics (ABX; neomycin, ampicillin, metronidazole; each 1 g/l) in drinking water for 2 weeks. On the 14th day, colonic motility was measured and the next day intestinal transit was assessed using charcoal propagation. Trunk blood, liver and intestine samples were removed for biochemical and histopathological evaluations, and faeces were collected for microbiota analysis. A 2-week HFD feeding increased blood glucose level and perirenal fat weight, induced low-level hepatic and intestinal inflammation, delayed intestinal transit, led to deterioration of epithelial tight junctions and overgrowth of colonic bacteria. Melatonin intake in HFD-fed rats reduced ileal inflammation, colonic motility and perirenal fat accumulation. ABX abolished increases in fat accumulation and blood glucose, reduced ileal oxidative damage, suppressed HFD-induced overgrowth in colonic bacteria, and reversed HFD-induced delay in intestinal transit; however, hepatic neutrophil accumulation, hepatic injury and dysfunction were further enhanced. In conclusion, the results demonstrate that even a short-term HFD ingestion results in hepato-intestinal inflammatory state and alterations in bacterial populations, which may be worsened with antibiotic intake, but alleviated by melatonin.
Comparative transcriptome profiling reveals candidate genes related to insecticide resistance ofGlyphodes pyloalis
Authors: Su H. , Gao Y. , Liu Y. , Li X. , Liang Y. , Dai X. , Xu Y. , Zhou Y. , Wang H.
Published: June 2019

Glyphodes pyloalisWalker (Lepidoptera: Pyralididae) is a common pest in sericulture and has developed resistance to different insecticides. However, the mechanisms involved in insecticide resistance ofG. pyloalisare poorly understood. Here, we present the first whole-transcriptome analysis of differential expression genes in insecticide-resistant and susceptibleG. pyloalis. Clustering and enrichment analysis of DEGs revealed several biological pathways and enriched Gene Ontology terms were related to detoxification or insecticide resistance. Genes involved in insecticide metabolic processes, including cytochrome P450, glutathione S-transferases and carboxylesterase, were identified in the larval midgut ofG. pyloalis.Among them,CYP324A19,CYP304F17,CYP6AW1,CYP6AB10,GSTs5, andAChE-likewere significantly increased after propoxur treatment, whileCYP324A19,CCE001c, andAChE-likewere significantly induced by phoxim, suggesting that these genes were involved in insecticide metabolism. Furthermore, the sequence variation analysis identified 21 single nucleotide polymorphisms withinCYP9A20,CYP6AB47,andCYP6AW1. Our findings reveal many candidate genes related to insecticide resistance ofG. pyloalis. These results provide novel insights into insecticide resistance and facilitate the development of insecticides with greater specificity toG. pyloalis.
Topological rigidity for closed hypersurfaces of elliptic space forms
Authors: Longa Eduardo Rosinato , Ripoll Jaime Bruck
Published: June 2019

We prove a topological rigidity theorem for closed hypersurfaces of the Euclidean sphere and of an elliptic space form. It asserts that, under a lower bound hypothesis on the absolute value of the principal curvatures, the hypersurface is diffeomorphic to a sphere or to a quotient of a sphere by a group action. We also prove another topological rigidity result for hypersurfaces of the sphere that involves the spherical image of its usual Gauss map.
Physical exercise may improve sleep quality in children and adolescents with Fontan circulation
Authors: Hedlund Eva R. , Villard Li , Lundell Bo , Sjöberg Gunnar
Published: June 2019

Objective:

To study physical activity and sleep in Fontan patients and healthy controls before and after an endurance training program, and after 1 year.

Method:

Fontan patients (n = 30) and healthy controls (n = 25) wore accelerometers for seven consecutive days and nights during a school week before and after a 12-week endurance training program and after 1 year.

Results:

Patients had similar sleep duration and sleep efficiency as healthy controls. Latency to sleep onset in minutes was longer for patients than controls (22.4 (4.3–55.3) minutes versus 14.8 (8.6–29.4) minutes, p < 0.01). More time in moderate-to-vigorous activity daytime was correlated with increased sleep time (p < 0.05; r2 = 0.20), improved sleep efficiency (p < 0.01; r2 = 0.24) and less time as wake after sleep onset (p < 0.05; r2 = 0.21) for patients but not controls. Sleep variables did not change after the exercise intervention for patients or controls. After 1 year, patients had decreased total sleep time, decreased sleep efficiency, increased accelerometer counts during sleep and more time as wake after sleep onset during sleep time, but not controls.

Conclusions:

Fontan patients have prolonged latency to sleep onset compared with controls. More time in physical activities was correlated with better sleep quality for the patients. Also, subjects with low sleep efficiency and long latency to sleep onset may benefit most from physical exercise. These patients should be encouraged to engage in individually designed physical exercise as this could improve sleep quality.
Risk factors for recidivism in individuals receiving community sentences: a systematic review and meta-analysis
Authors: Yukhnenko Denis , Blackwood Nigel , Fazel Seena
Published: June 2019

Objective.

We aimed to systematically review risk factors for criminal recidivism in individuals given community sentences.

Methods.

We searched seven bibliographic databases and additionally conducted targeted searches for studies that investigated risk factors for any repeat offending in individuals who had received community (non-custodial) sentences. We included investigations that reported data on at least one risk factor and allowed calculations of odds ratios (ORs). If a similar risk factor was reported in three or more primary studies, they were grouped into domains, and pooled ORs were calculated.

Results.

We identified 15 studies from 5 countries, which reported data on 14 independent samples and 246,608 individuals. We found that several dynamic (modifiable) risk factors were associated with criminal recidivism in community-sentenced populations, including mental health needs (OR = 1.4, 95% confidence interval (CI): 1.2–1.6), substance misuse (OR = 2.3, 95% CI: 1.1–4.9), association with antisocial peers (OR = 2.2, 95% CI: 1.3–3.7), employment problems (OR = 1.8, 95% CI: 1.3–2.5), marital status (OR = 1.6, 95%: 1.4–1.8), and low income (OR = 2.0, 95% CI: 1.1–3.4). The strength of these associations was comparable to that of static (non-modifiable) risk factors, such as age, gender, and criminal history.

Conclusion.

Assessing dynamic (modifiable) risk factors should be considered in all individuals given community sentences. The further integration of mental health, substance misuse, and criminal justice services may reduce reoffending risk in community-sentenced populations.
Radiotherapy students’ perceptions of support provided by clinical supervisors
Authors: Armstrong-James L. , Khine R. N. M. , Thorne R. J. , Tuckey M. J. E. , Bennett C.
Published: June 2019

Aim:

The aim of this study was to explore the experiences of radiotherapy students on clinical placement, specifically focussing on the provision of well-being support from clinical supervisors.

Materials and methods:

Twenty-five students from the University of the West of England and City University of London completed an online evaluation survey relating to their experiences of placement, involving Likert scales and open-ended questions.

Results:

The quantitative results were generally positive; however, the qualitative findings were mixed. Three themes emerged: (1) provision of information and advice; (2) an open, inclusive and supportive working environment; and (3) a lack of communication, understanding, and consistency.

Findings:

Students’ experiences on placement differed greatly and appeared to relate to their specific interactions with different members of staff. It is suggested that additional training around providing well-being support to students may be of benefit to clinical supervisors.
Establishing a three-generation prospective study: Bogalusa daughters
Authors: Harville EW , Breckner D , Shu T , Cooper M , Bazzano LA
Published: June 2019

Objective:

The Developmental Origins of Disease hypothesis has spurred increased interest in how prenatal exposures affect lifelong health, while mechanisms such as epigenetics may explain the multigenerational influences on health. Such factors are not well captured within conventional epidemiologic study designs. We explored the feasibility of collecting information on the offspring and grand-offspring of participants in a long-running study.

Design:

The Bogalusa Heart Study is a study, begun in 1973, of life-course cardiovascular health in a semirural population (65% white and 35% black).

Main measures:

Female participants who had previously provided information on their pregnancies were contacted to obtain contact information for their daughters aged 12 and older. Daughters were then contacted to obtain reproductive histories, and invited for a clinic or lab visit to measure cardiovascular risk factors.

Results:

Two hundred seventy-four daughters of 208 mothers were recruited; 81% (223) had a full clinic visit and 19% (51) a phone interview only. Forty-five percent of the daughters were black, and 55% white. Mean and median age at interview was 27, with 15% under the age of 18. The strongest predictors of participation were black race, recent maternal participation in the parent study, and living in or near Bogalusa. Simple correlations for cardiovascular risk factors across generations were between r = 0.19 (systolic blood pressure) and r = 0.39 (BMI, LDL).

Conclusion:

It is feasible to contact the children of study participants even when participants are adults, and initial information on the grandchildren can also be determined in this manner.
Herbicide options for glyphosate-resistant kochia (Bassia scoparia) management in the Great Plains
Authors: Sbatella Gustavo M. , Adjesiwor Albert T. , Kniss Andrew R. , Stahlman Phillip W. , Westra Phil , Moechnig Michael , Wilson Robert G.
Published: June 2019

Kochia is one of the most problematic weeds in the United States. Field studies were conducted in five states (Wyoming, Colorado, Kansas, Nebraska, and South Dakota) over 2 yr (2010 and 2011) to evaluate kochia control with selected herbicides registered in five common crop scenarios: winter wheat, fallow, corn, soybean, and sugar beet to provide insight for diversifying kochia management in crop rotations. Kochia control varied by experimental site such that more variation in kochia control and biomass production was explained by experimental site than herbicide choice within a crop. Kochia control with herbicides currently labeled for use in sugar beet averaged 32% across locations. Kochia control was greatest and most consistent from corn herbicide programs (99%), followed by soybean (96%) and fallow (97%) herbicide programs. Kochia control from wheat herbicide programs was 93%. With respect to the availability of effective herbicide options, glyphosate-resistant kochia control was easiest in corn, soybean, and fallow, followed by wheat; and difficult to manage with herbicides in sugar beet.
Quantifying the Impacts of Cold Airmass on Aerosol Concentrations Over North China Using Isentropic Analysis
Authors: Liu Qian , Chen Guixing , Iwasaki Toshiki
Published: June 2019

Winter air quality can vary markedly due to cold air activities. However, the impacts of the polar cold airmass on aerosol variations has not been clarified in a quantitative manner. Using an isentropic analysis method, we investigated changes in the cold airmass and sulfate aerosol distributions over North China during the winter of 2014/2015. The cold airmass and sulfate concentrations exhibited pronounced out‐of‐phase variations, with comparable amplitudes at subseasonal (30–60 days) and synoptic (4–6 days) scales. Subseasonal sulfate variations were closely associated with distribution of the polar cold airmass, as it shifted between zonal and meridional patterns, regulating cold airmass fluxes over North China. Typically, subseasonal surges in the cold airmass consisted of several synoptic disturbances, including one cold air outbreak event and 3–6 renewal/decay oscillations. These synoptic inflows of clean cold airmass repeatedly pushed the warm polluted airmass away from North China. Thus, spatiotemporal variations in sulfate better reflect cold airmass distributions and fluxes than northerly wind regimes. Using diagnostic equations derived herein, we estimated the contribution of various physical processes to aerosol variations during cold air anomalies. We found that the local changes in sulfate concentrations were mainly attributed to advection of warm airmasses, as well as advection and vertical displacement by horizontal convergence of the polar cold airmass. The latter two processes contributed most to sulfate decreases, leading to a relatively strong aerosol reductions during cold air outbreaks, while first two processes led to weaker aerosol reductions during cold air oscillations.
Tropopause Laminar Cirrus and Its Role in the Lower Stratosphere Total Water Budget
Authors: Wang Tao , Wu Dong L. , Gong Jie , Tsai Victoria
Published: June 2019

Laminar cirrus are thin, extensive, isolated layers of ice clouds frequently observed in the tropical tropopause layer. Widespread laminar cirrus significantly affects tropical tropopause layer total water and thermal budget. In this study, we extract laminar cirrus from the Cloud‐Aerosol Lidar with Orthogonal Polarization Level 1 attenuated total backscatter images for January 2009, in order to characterize statistical properties of laminar cirrus cloud length, base, thickness, optical depth, and layer partial ice water path. These characteristics are used to develop an algorithm identifying laminar cirrus automatically from the Cloud‐Aerosol Lidar with Orthogonal Polarization Level 2 layer product for 2008–2017. The nearly 10‐year records reveal that tropopause laminar cirrus occurrence (30–40% of total cirrus) is strongly anticorrelated with the tropopause temperatures in that colder tropopause in frequent (super)saturation during boreal winter favors in situ formation of clouds. Interannually, anomalously warmer troposphere temperature (ΔT), easterly shear of the quasi‐biennial oscillation, and stronger upwelling branch of the Brewer‐Dobson circulation enhance laminar cirrus formation via cooling of the tropopause. The tropopause laminar cirrus carries ~0.05 mg/m3 (~0.5 ppmv) of ice water content during boreal winter and <0.01 mg/m3 during summer, which is anticorrelated with the seasonal variations of water vapor (H2O) observed by the Microwave Lime Sounder, indicating a temperature‐regulated partition between vapor and ice. Interannually, in cirrus‐rich region 1 ppmv decrease in H2O corresponds to 0.2–0.3 ppmv increase in ice water content. Frequently situated in (super)saturated air, tropopause laminar cirrus are likely to survive multiple lifecycles of the sublimation‐deposition processes, and may contribute up to 10% to the total water budget in the lower stratosphere.
Detection and Removal of Clouds and Associated Shadows in Satellite Imagery Based on Simulated Radiance Fields
Authors: Wang Tianxing , Shi Jiancheng , Letu Husi , Ma Ya , Li Xingcai , Zheng Yaomin
Published: June 2019

Clouds and shadows pose a significant barrier for land surface optical and infrared remote sensing image processing and their various applications. The detection and removal of clouds and shadows from satellite images have always been critical preprocessing steps. To date, a variety of methods have been designed to solve this problem. Some require particular channels, while others are heavily dependent on the availability of temporally adjacent images (reference images). Moreover, many methods are too complex to use by common users. For those reasons, in this paper an alternative scheme for detecting clouds and shadows is proposed based on simulated top‐of‐atmosphere radiance fields. At the same time, a simple approach to remove clouds and shadows is also provided. The results indicate that the new method can properly identify both clouds and shadows in satellite images. Especially, it shows obvious advantage over the Moderate Resolution Imaging Spectroradiometer cloud product (MOD35) for shadow detection. Although the proposed cloud removal method is simple, the radiances of a contaminated image can be reasonably reconstructed with root‐mean‐square error < 3.0 W/m2·sr·μm and mean bias < 1.0 W/m2·sr·μm for all seven Moderate Resolution Imaging Spectroradiometer reflective bands for our case studies. These results prove the effectiveness of the proposed scheme in identifying and removing clouds and shadows from remotely sensed images. Meanwhile, these findings provide some new ideas for the remote sensing community, especially in the fields of cloud detection and image processing.
Measuring River Wetted Width From Remotely Sensed Imagery at the Subpixel Scale With a Deep Convolutional Neural Network
Authors: Ling Feng , Boyd Doreen , Ge Yong , Foody Giles M. , Li Xiaodong , Wang Lihui , Zhang Yihang , Shi Lingfei , Shang Cheng , Li Xinyan , Du Yun
Published: June 2019

River wetted width (RWW) is an important variable in the study of river hydrological and biogeochemical processes. Presently, RWW is often measured from remotely sensed imagery, and the accuracy of RWW estimation is typically low when coarse spatial resolution imagery is used because river boundaries often run through pixels that represent a region that is a mixture of water and land. Thus, when conventional hard classification methods are used in the estimation of RWW, the mixed pixel problem can become a large source of error. To address this problem, this paper proposes a novel approach to measure RWW at the subpixel scale. Spectral unmixing is first applied to the imagery to obtain a water fraction image that indicates the proportional coverage of water in image pixels. A fine spatial resolution river map from which RWW may be estimated is then produced from the water fraction image by superresolution mapping (SRM). In the SRM analysis, a deep convolutional neural network is used to eliminate the negative effects of water fraction errors and reconstruct the geographical distribution of water. The proposed approach is assessed in two experiments, with the results demonstrating that the convolutional neural network‐based SRM model can effectively estimate subpixel scale details of rivers and that the accuracy of RWW estimation is substantially higher than that obtained from the use of a conventional hard image classification. The improvement shows that the proposed method has great potential to derive more accurate RWW values from remotely sensed imagery.
Climate Impacts From Large Volcanic Eruptions in a High‐Resolution Climate Model: The Importance of Forcing Structure
Authors: Yang Wenchang , Vecchi Gabriel A. , Fueglistaler Stephan , Horowitz Larry W. , Luet David J. , Muñoz Ángel G. , Paynter David , Underwood Seth
Published: June 2019

Explosivevolcanic eruptions have large climate impacts and can serve as observable tests of the climatic response to radiative forcing. Using a high‐resolution climate model, we contrast the climate responses to Pinatubo, with symmetric forcing, and those to Santa Maria and Agung, which had meridionally asymmetric forcing. Although Pinatubo had larger global‐mean forcing, asymmetric forcing strongly shifts the latitude of tropical rainfall features, leading to larger local precipitation/tropical cyclone changes. For example, North Atlantic tropical cyclone activity over is enhanced/reduced by SH forcing (Agung)/NH forcing (Santa Maria) but changes little in response to the Pinatubo forcing. Moreover, the transient climate sensitivity estimated from the response to Santa Maria is 20% larger than that from Pinatubo or Agung. This spread in climatic impacts of volcanoes needs to be considered when evaluating the role of volcanoes in global and regional climate and serves to contextualize the well‐observed response to Pinatubo.
ENSO Regime Changes Responsible for Decadal Phase Relationship Variations Between ENSO Sea Surface Temperature and Warm Water Volume
Authors: Zhang Wenjun , Li Sixu , Jin Fei‐Fei , Xie Ruihuang , Liu Chao , Stuecker Malte F. , Xue Aoyun
Published: June 2019

The relationship between the equatorial Pacific warm water volume (WWV) and El Niño–Southern Oscillation (ENSO) sea surface temperature (SST) has varied considerably on decadal timescales. These changes are strongly related to the occurrence frequency of central Pacific (CP) ENSO events. While both eastern Pacific (EP) and CP ENSO events show clear signatures of WWV recharge/discharge, their phase‐lag relationships between WWV and Niño3.4 SST are different. The WWV usually leads the Niño3.4 SST by two to three seasons during EP ENSO, while the lead time is reduced to one season during CP ENSO. The different phase‐lag relationships can be explained by distinct periodicities of the two ENSO types. Hence, ENSO regime changes associated with decadal predominance of either EP or CP ENSO events can give rise to decadal variations in the statistical WWV‐ENSO SST relationship. We emphasize the importance of identifying these different ENSO types and potentially different ENSO regimes to assess ENSO predictability.
Author Correction: Human mesenchymal stem cells promote tumor growth via MAPK pathway and metastasis by epithelial mesenchymal transition and integrin α5 in hepatocellular carcinoma
Authors: Chen Jiang , Ji Tong , Wu Di , Jiang Shi , Zhao Jie , Lin Hui , Cai Xiujun
Published: June 2019

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
An injectable, click-crosslinked, cytomodulin-modified hyaluronic acid hydrogel for cartilage tissue engineering
Authors: Park Seung Hun , Seo Ji Young , Park Joon Yeong , Ji Yun Bae , Kim Kyungsook , Choi Hak Soo , Choi Sangdun , Kim Jae Ho , Min Byoung Hyun , Kim Moon Suk
Published: June 2019

This is the first report, to our knowledge, of the preparation of an injectable in situ–forming click-crosslinked hyaluronic acid (Cx-HA) hydrogel (Cx-HA-CM) containing chemical immobilized cytomodulin-2 (CM), a chondrogenic differentiation factor, and on the utility of human periodontal ligament stem cells (hPLSCs) as a cell source for cartilage tissue engineering. hPLSCs served here as a stem cell source tolerant to ex vivo manipulation. CM induced in vitro chondrogenic differentiation of hPLSCs comparable to induction with traditional TGF-β. Cx-HA was prepared via a click-reaction between tetrazine-modified HA and transcyclooctene-modified HA. Cx-HA displayed significantly more features of a stiff hydrogel than HA. Cx-HA had a three-dimensional porous interconnected structure, absorbed a large volume of biological medium, and showed excellent biocompatibility. In contrast to HA, the Cx-HA hydrogel persisted in vitro and in vivo for an extended period, as evidenced by in vivo near-infrared fluorescence imaging. CM covalently linked to Cx-HA (Cx-HA-CM) remained inside Cx-HA for a prolonged period compared with CM physically loaded onto Cx-HA [Cx-HA (+CM)]. Cx-HA-CM also caused better chondrogenic differentiation of hPLSCs, as evidenced by Alcian blue and Safranin O staining, and greater increases in the expression of type II collagen, glycosaminoglycan content and SOX9, aggrecan, and type 2α1 collagen mRNA levels. Thus, compared to Cx-HA (+CM), the hPLSC-loaded Cx-HA-CM hydrogel induced greater chondrogenic differentiation of hPLSCs via CM that was retained in the hydrogel for a much longer period of time.
The Lyapunov spectra of quantum thermalisation
Authors: Hallam A. , Morley J. G. , Green A. G.
Published: June 2019

Thermalisation in closed quantum systems occurs through a process of dephasing due to parts of the system outside of the window of observation, gradually revealing the underlying thermal nature of eigenstates. In contrast, closed classical systems thermalize due to dynamical chaos. We demonstrate a deep link between these processes. Projecting quantum dynamics onto variational states using the time-dependent variational principle, results in classical chaotic Hamiltonian dynamics. We study an infinite spin chain in two ways—using the matrix product state ansatz for the wavefunction and for the thermofield purification of the density matrix—and extract the full Lyapunov spectrum of the resulting dynamics. We show that the entanglement growth rate is related to the Kolmogorov–Sinai entropy of dynamics projected onto states with appropriate entanglement, extending previous results about initial entanglement growth to all times. The Lyapunov spectra for thermofield descriptions of thermalizing systems show a remarkable semi-circular distribution.
Field-programmable silicon temporal cloak
Authors: Zhou Feng , Yan Siqi , Zhou Hailong , Wang Xu , Qiu Huaqing , Dong Jianji , Zhou Linjie , Ding Yunhong , Qiu Cheng-Wei , Zhang Xinliang
Published: June 2019

Temporal cloaks have aroused tremendous research interest in both optical physics and optical communications, unfolding a distinct approach to conceal temporal events from an interrogating optical field. The state-of-the-art temporal cloaks exhibit picosecond-scale and static cloaking window, owing to significantly limited periodicity and aperture of time lens. Here we demonstrate a field-programmable silicon temporal cloak for hiding nanosecond-level events, enabled by an integrated silicon microring and a broadband optical frequency comb. With dynamic control of the driving electrical signals on the microring, our cloaking windows could be stretched and switched in real time from 0.449 ns to 3.365 ns. Such a field-programmable temporal cloak may exhibit practically meaningful potentials in secure communication, data compression, and information protection in dynamically varying events.
Regulating intracellular fate of siRNA by endoplasmic reticulum membrane-decorated hybrid nanoplexes
Authors: Qiu Chong , Han Hu-Hu , Sun Jing , Zhang Hai-Tao , Wei Wei , Cui Shi-He , Chen Xin , Wang Jian-Cheng , Zhang Qiang
Published: June 2019

Most cationic vectors are difficult to avoid the fate of small interfering RNA (siRNA) degradation following the endosome-lysosome pathway during siRNA transfection. In this study, the endoplasmic reticulum (ER) membrane isolated from cancer cells was used to fabricate an integrative hybrid nanoplexes (EhCv/siRNA NPs) for improving siRNA transfection. Compared to the undecorated Cv/siEGFR NPs, the ER membrane-decorated EhCv/siRNA NPs exhibits a significantly higher gene silencing effect of siRNA in vitro and a better antitumor activity in nude mice bearing MCF-7 human breast tumor in vivo. Further mechanistic studies demonstrate that functional proteins on the ER membrane plays important roles on improving cellular uptake and altering intracellular trafficking pathway of siRNA. It is worth to believe that the ER membrane decoration on nanoplexes can effectively transport siRNA through the endosome-Golgi-ER pathway to evade lysosomal degradation and enhance the silencing effects of siRNA.
CD46 facilitates entry and dissemination of human cytomegalovirus
Authors: Stein Kathryn R. , Gardner Thomas J. , Hernandez Rosmel E. , Kraus Thomas A. , Duty James A. , Ubarretxena-Belandia Iban , Moran Thomas M. , Tortorella Domenico
Published: June 2019

Human cytomegalovirus (CMV) causes a wide array of disease to diverse populations of immune-compromised individuals. Thus, a more comprehensive understanding of how CMV enters numerous host cell types is necessary to further delineate the complex nature of CMV pathogenesis and to develop targeted therapeutics. To that end, we establish a vaccination strategy utilizing membrane vesicles derived from epithelial cells to generate a library of monoclonal antibodies (mAbs) targeting cell surface proteins in their native conformation. A high-throughput inhibition assay is employed to screen these antibodies for their ability to limit infection, and mAbs targeting CD46 are identified. In addition, a significant reduction of viral proliferation in CD46-KO epithelial cells confirms a role for CD46 function in viral dissemination. Further, we demonstrate a CD46-dependent entry pathway of virus infection in trophoblasts, but not in fibroblasts, highlighting the complexity of CMV entry and identifying CD46 as an entry factor in congenital infection.
Metabolic adaptability in metastatic breast cancer by AKR1B10-dependent balancing of glycolysis and fatty acid oxidation
Authors: van Weverwijk Antoinette , Koundouros Nikolaos , Iravani Marjan , Ashenden Matthew , Gao Qiong , Poulogiannis George , Jungwirth Ute , Isacke Clare M.
Published: June 2019

The different stages of the metastatic cascade present distinct metabolic challenges to tumour cells and an altered tumour metabolism associated with successful metastatic colonisation provides a therapeutic vulnerability in disseminated disease. We identify the aldo-keto reductase AKR1B10 as a metastasis enhancer that has little impact on primary tumour growth or dissemination but promotes effective tumour growth in secondary sites and, in human disease, is associated with an increased risk of distant metastatic relapse. AKR1B10High tumour cells have reduced glycolytic capacity and dependency on glucose as fuel source but increased utilisation of fatty acid oxidation. Conversely, in both 3D tumour spheroid assays and in vivo metastasis assays, inhibition of fatty acid oxidation blocks AKR1B10High-enhanced metastatic colonisation with no impact on AKR1B10Low cells. Finally, mechanistic analysis supports a model in which AKR1B10 serves to limit the toxic side effects of oxidative stress thereby sustaining fatty acid oxidation in metabolically challenging metastatic environments.
Phosphorylated lipid-conjugated oligonucleotide selectively anchors on cell membranes with high alkaline phosphatase expression
Authors: Jin Cheng , He Jiaxuan , Zou Jianmei , Xuan Wenjing , Fu Ting , Wang Ruowen , Tan Weihong
Published: June 2019

Attachment of lipid tails to oligonucleotides has emerged as a powerful technology in constructing cell membrane-anchorable nucleic acid-based probes. In practice, however, conventional lipid-conjugated oligonucleotides fail to distinguish among different cell membranes. Herein, a phosphorylated lipid-conjugated oligonucleotide (DNA-lipid-P) is reported for alkaline phosphatase (ALP)-dependent cell membrane adhesion. In the absence of ALP, DNA-lipid-P with its poor hydrophobicity shows only weak interaction with cell membrane. However, in the presence of the highly expressed plasma membrane-associated ALP, DNA-lipid-P is converted to lipid-conjugated oligonucleotide (DNA-lipid) by enzymatic dephosphorylation. As a result of such conversion, the generated DNA-lipid has greater hydrophobicity than DNA-lipid-P and is thus able to insert into cell membranes in situ. Accordingly, DNA-lipid-P enables selective anchoring on cell membranes with elevated ALP level. Since elevated ALP level is a critical index of some diseases and even cancers, DNA-lipid-P holds promise for cell membrane engineering and disease diagnostics at the molecular level.
Direct allylic C–H alkylation of enol silyl ethers enabled by photoredox–Brønsted base hybrid catalysis
Authors: Ohmatsu Kohsuke , Nakashima Tsubasa , Sato Makoto , Ooi Takashi
Published: June 2019

Strategies for altering the reaction pathway of reactive intermediates are of significant importance in diversifying organic synthesis. Enol silyl ethers, versatile enolate equivalents, are known to undergo one-electron oxidation to generate the radical cations that spontaneously form electrophilic α-carbonyl radicals via elimination of the silyl groups. Here, we demonstrate that close scrutiny of the property of the radical cations as strong C–H acids enables the identification of a catalyst system consisting of an iridium-based photosensitizer and 2,4,6-collidine for the generation of nucleophilic allylic radicals from enol silyl ethers through one-electron oxidation-deprotonation sequence under light irradiation without the desilylation of the radical cation intermediates. The resultant allylic radicals engage in the addition to electron-deficient olefins, establishing the selective allylic C-H alkylation of enol silyl ethers. This strategy is broadly applicable, and the alkylated enol silyl ethers can be transformed into highly functionalized carbonyl compounds by exploiting their common polar reactivity.
Non-equilibrium dynamics of a nascent polypeptide during translation suppress its misfolding
Authors: Alexander Lisa M. , Goldman Daniel H. , Wee Liang M. , Bustamante Carlos
Published: June 2019

Protein folding can begin co-translationally. Due to the difference in timescale between folding and synthesis, co-translational folding is thought to occur at equilibrium for fast-folding domains. In this scenario, the folding kinetics of stalled ribosome-bound nascent chains should match the folding of nascent chains in real time. To test if this assumption is true, we compare the folding of a ribosome-bound, multi-domain calcium-binding protein stalled at different points in translation with the nascent chain as is it being synthesized in real-time, via optical tweezers. On stalled ribosomes, a misfolded state forms rapidly (1.5 s). However, during translation, this state is only attained after a long delay (63 s), indicating that, unexpectedly, the growing polypeptide is not equilibrated with its ensemble of accessible conformations. Slow equilibration on the ribosome can delay premature folding until adequate sequence is available and/or allow time for chaperone binding, thus promoting productive folding.
The Polycomb protein Ezl1 mediates H3K9 and H3K27 methylation to repress transposable elements in Paramecium
Authors: Frapporti Andrea , Miró Pina Caridad , Arnaiz Olivier , Holoch Daniel , Kawaguchi Takayuki , Humbert Adeline , Eleftheriou Evangelia , Lombard Bérangère , Loew Damarys , Sperling Linda , Guitot Karine , Margueron Raphaël , Duharcourt Sandra
Published: June 2019

In animals and plants, the H3K9me3 and H3K27me3 chromatin silencing marks are deposited by different protein machineries. H3K9me3 is catalyzed by the SET-domain SU(VAR)3–9 enzymes, while H3K27me3 is catalyzed by the SET-domain Enhancer-of-zeste enzymes, which are the catalytic subunits of Polycomb Repressive Complex 2 (PRC2). Here, we show that the Enhancer-of-zeste-like protein Ezl1 from the unicellular eukaryote Paramecium tetraurelia, which exhibits significant sequence and structural similarities with human EZH2, catalyzes methylation of histone H3 in vitro and in vivo with an apparent specificity toward K9 and K27. We find that H3K9me3 and H3K27me3 co-occur at multiple families of transposable elements in an Ezl1-dependent manner. We demonstrate that loss of these histone marks results in global transcriptional hyperactivation of transposable elements with modest effects on protein-coding gene expression. Our study suggests that although often considered functionally distinct, H3K9me3 and H3K27me3 may share a common evolutionary history as well as a common ancestral role in silencing transposable elements.
Lasing in strained germanium microbridges
Authors: Armand Pilon F. T. , Lyasota A. , Niquet Y.-M. , Reboud V. , Calvo V. , Pauc N. , Widiez J. , Bonzon C. , Hartmann J. M. , Chelnokov A. , Faist J. , Sigg H.
Published: June 2019

Germanium has long been regarded as a promising laser material for silicon based opto-electronics. It is CMOS-compatible and has a favourable band structure, which can be tuned by strain or alloying with Sn to become direct, as it was found to be required for interband semiconductor lasers. Here, we report lasing in the mid-infrared region (from λ = 3.20 μm up to λ = 3.66 μm) in tensile strained Ge microbridges uniaxially loaded above 5.4% up to 5.9% upon optical pumping, with a differential quantum efficiency close to 100% with a lower bound of 50% and a maximal operating temperature of 100 K. We also demonstrate the effect of a non-equilibrium electron distribution in k-space which reveals the importance of directness for lasing. With these achievements the strained Ge approach is shown to compare well to GeSn, in particular in terms of efficiency.
Simultaneous motor preparation and execution in a last-moment reach correction task
Authors: Ames K. Cora , Ryu Stephen I. , Shenoy Krishna V.
Published: June 2019

Motor preparation typically precedes movement and is thought to determine properties of upcoming movements. However, preparation has mostly been studied in point-to-point delayed reaching tasks. Here, we ask whether preparation is engaged during mid-reach modifications. Monkeys reach to targets that occasionally jump locations prior to movement onset, requiring a mid-reach correction. In motor cortex and dorsal premotor cortex, we find that the neural activity that signals when to reach predicts monkeys’ jump responses on a trial-by-trial basis. We further identify neural patterns that signal where to reach, either during motor preparation or during motor execution. After a target jump, neural activity responds in both preparatory and movement-related dimensions, even though error in preparatory dimensions can be small at that time. This suggests that the same preparatory process used in delayed reaching is also involved in reach correction. Furthermore, it indicates that motor preparation and execution can be performed simultaneously.