bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Peptides Originating from Expression Libraries of Aurelia aurita and Mnemiopsis leidyi Prevent Biofilm Formation of Opportunistic Pathogens
Authors: Ladewig Lisa , Gloy Leon , Langfeldt Daniela , Pinnow Nicole , Weiland-Bräuer Nancy , Schmitz Ruth A.
Published: August 2023

The demand for novel antimicrobial compounds is rapidly growing due to the rising appearance of antibiotic resistance in bacteria; accordingly, alternative approaches are urgently needed. Antimicrobial peptides (AMPs) are promising, since they are a naturally occurring part of the innate immune system and display remarkable broad-spectrum activity and high selectivity against various microbes. Marine invertebrates are a primary resource of natural AMPs. Consequently, cDNA expression (EST) libraries from the Cnidarian moon jellyfish Aurelia aurita and the Ctenophore comb jelly Mnemiopsis leidyi were constructed in Escherichia coli. Cell-free size-fractionated cell extracts (<3 kDa) of the two libraries (each with 29,952 clones) were consecutively screened for peptides preventing the biofilm formation of opportunistic pathogens using the crystal violet assay. The 3 kDa fraction of ten individual clones demonstrated promising biofilm-preventing activities against Klebsiella oxytoca and Staphylococcus epidermidis. Sequencing the respective activity-conferring inserts allowed for the identification of small ORFs encoding peptides (10–22 aa), which were subsequently chemically synthesized to validate their inhibitory potential. Although the peptides are likely artificial products from a random translation of EST inserts, the biofilm-preventing effects against K. oxytoca, Pseudomonas aeruginosa, S. epidermidis, and S. aureus were verified for five synthetic peptides in a concentration-dependent manner, with peptide BiP_Aa_5 showing the strongest effects. The impact of BiP_Aa_2, BiP_Aa_5, and BiP_Aa_6 on the dynamic biofilm formation of K. oxytoca was further validated in microfluidic flow cells, demonstrating a significant reduction in biofilm thickness and volume by BiP_Aa_2 and BiP_Aa_5. Overall, the structural characteristics of the marine invertebrate-derived AMPs, their physicochemical properties, and their promising antibiofilm effects highlight them as attractive candidates for discovering new antimicrobials.
Nutraceutical Combinational Therapy for Diarrhoea Control with Probiotic Beverages from Fermented Fruits, Vegetables and Cereals to Regain Lost Hydration, Nutrition and Gut Microbiota
Authors: Dahiya Divakar , Nigam Poonam Singh
Published: August 2023

This article deals with the condition of irregular bowel movements known as diarrhoea, its pathology, symptoms and aetiology. The information has been presented on causes of diarrhoea that include gut infections, food intolerances and allergies to certain ingredients, problems in the gastrointestinal tract like irritable bowel syndrome, inflammatory bowel disease and, the condition of dysbiosis which occurs due to long-term use of antibiotics, or other medicines, etc. Most cases of diarrhoea can be resolved without needing medical treatment; however, it is still important to avoid dehydration of the body and use some supplements to get necessary nutrients which are lost with frequent bowel movements before they can get absorbed and assimilated in the gastrointestinal tract. Probiotic products are reported as natural therapeutic agents, which can reduce the risk of diarrhoea in both adults and children. The intake of dietary fluid supplements in the form of fermented beverages containing probiotic strains could help in diarrhoea control. The patient would achieve benefits with the consumption of these functional beverages in three ways—by regaining lost fluids to the body, supplementing beneficial gut bacteria to restore diversity in gut microbiota, which was disturbed in the condition of diarrhoea as well as regaining a source of quick nutrition to recoup energy.
Changes in the Ultrastructure of Staphylococcus aureus Cells Make It Possible to Identify and Analyze the Injuring Effects of Ciprofloxacin, Polycationic Amphiphile and Their Hybrid
Authors: Grigor’eva Alina E. , Bardasheva Alevtina V. , Ryabova Elena S. , Tupitsyna Anastasiya V. , Zadvornykh Danila A. , Koroleva Lyudmila S. , Silnikov Vladimir N. , Tikunova Nina V. , Ryabchikova Elena I.
Published: August 2023

The purposeful development of synthetic antibacterial compounds requires an understanding of the relationship between effects of compounds and their chemical structure. This knowledge can be obtained by studying changes in bacteria ultrastructure under the action of antibacterial compounds of a certain chemical structure. Our study was aimed at examination of ultrastructural changes in S. aureus cells caused by polycationic amphiphile based on 1,4‒diazabicyclo[2.2.2]octane (DL412), ciprofloxacin and their hybrid (DL5Cip6); the samples were incubated for 15 and 45 min. DL412 first directly interacted with bacterial cell wall, damaging it, then penetrated into the cell and disrupted cytoplasm. Ciprofloxacin penetrated into cell without visually damaging the cell wall, but altered the cell membrane and cytoplasm, and inhibited the division of bacteria. The ultrastructural characteristics of S. aureus cells damaged by the hybrid clearly differed from those under ciprofloxacin or DL412 action. Signs associated with ciprofloxacin predominated in cell damage patterns from the hybrid. We studied the effect of ciprofloxacin, DL412 and their hybrid on S. aureus biofilm morphology using paraffin sections. Clear differences in compound effects on S. aureus biofilm (45 min incubation) were observed. The results obtained allow us to recommend this simple and cheap approach for the initial assessment of antibiofilm properties of synthesized compounds.
Electrocoagulation Combined with Synthetic Zeolite—Does the Size of Zeolite Particles Matter?
Authors: Vukojević Medvidović Nediljka , Vrsalović Ladislav , Svilović Sandra , Magaš Klara , Jozić Dražan , Čović Anđela
Published: August 2023

Although electrocoagulation combined with zeolite (ECZ) shows higher efficiency in wastewater treatment, the actual contribution of zeolite particle size has not been fully explored. In this work, the influence of particle size of synthetic zeolite SZ (<90, 90–160, and 160–600 μm) on ECZ treatment of compost leachate with very high organic load is investigated together with different electrode materials (Fe, Al, and Zn), current densities (0.003, 0.009 and 0.018 A/cm2), and contact times (10, 20 and 30 min). The results positively highlight that the largest particle size should be used in ECZ, as it leads to a lower increase in pH and temperature, a higher decrease of chemical oxygen demand (COD) and turbidity, and a lower electrode consumption, while causing more damage to the electrode surface. The estimated energy costs ranged from 3.960 kW/m3–1313.657 kW/m3. The Taguchi L9 orthogonal configuration showed the highest COD and turbidity decrease under the conditions of 160–600 µm zeolite particles. The powder X-ray diffractometer (PXRD) analysis shows that interplanar spacing decreases when smaller and medium SZ particle sizes are used, while this effect was not observed with larger zeolite particle size. SEM-EDS shows that oxygen, silicon, and aluminium are the predominant elements in electrogenerated sludge coupled with zeolite.
The Mid-Cretaceous Tectonothermal Evolution of the Zhangbaling Tectonic Belt, East China: Evidence from Zircon (U–Th)/He and Detrital Zircon U–Pb Dating
Authors: Wang Yongsheng , Bai Qiao , Ma Weiwei , Yang Juanhao , Li Zhensheng
Published: August 2023

The Zhangbaling tectonic belt (ZTB), one of the most representative tectonic belts in East China, has experienced uplift since the Early Cretaceous and is, thus, an excellent object for understanding the tectonic uplift and topographical evolution of East China and the whole of East Asia. On the basis of field observations, in this contribution to the literature, we carried out detrital zircon LA-ICP-MS U–Pb dating for the Upper Cretaceous sediments in the basins adjacent to the ZTB and zircon (U–Th)/He dating for the Early Cretaceous plutons along the western flank of this belt. Detailed field observation showed that the orthogneiss of the Feidong Complex experienced sinistral strike–slip activities, while the marbles underwent thrusting; thrust faults were developed in the Early Cretaceous plutons and volcanic rocks, and normal faults were superimposed on thrust or strike–slip faults. The detrital zircon dating results showed that the Upper Cretaceous sediments are characterized by an Early Cretaceous major cluster with just a minor cluster from the middle Neoproterozoic ages, indicating that the Zhangbaling Group and the Feidong Complex of the ZTB are not their main provenance. Zircon (U–Th)/He dating results showed that the ZTB experienced rapid uplifting during the mid-Cretaceous and recorded another rapid uplifting after 30 Ma. Combining existing research with our new data, it can be concluded that the ZTB was characterized by thrust activity in the mid-Cretaceous, which occurred under regional compression setting and was the basis of the formation of a watershed after 30 Ma.
Characterizing Archaeological Rhyolites in the Nenana Valley, Interior Alaska
Authors: Gore Angela K. , Graf Kelly , Lynch Joshua J.
Published: August 2023

Portable X-ray fluorescence (pXRF) is a useful geochemical technique employed to explore toolstone procurement strategies in the lithic record, commonly utilized in sourcing obsidians. Non-obsidian volcanic toolstones (e.g., dacites, rhyolites, basalts, and andesites) are abundant in interior Alaskan assemblages yet understudied compared to obsidian. Geochemical analyses of these non-obsidian materials offer the potential to gain new insights into ancient toolstone provisioning behaviors. This paper presents a synthesis of geochemical (pXRF) analyses of rhyolite artifacts, systematic regional raw material surveys, and lithic technological analyses collected from nineteen late Pleistocene and Holocene assemblages from the Nenana valley, interior Alaska. Previous research studies on archaeological rhyolites from the region are replicated, new rhyolite artifact groups are identified, and one new rhyolite source is reported and described here. Ultimately, this paper contributes to a growing body of geochemical research seeking to provide a more nuanced look at the complex late Pleistocene and Holocene record of eastern Beringia.
Awaruite, a New Large Nickel Resource: Flotation under Weakly Acidic Conditions
Authors: Seiler Santiago , Sánchez Gustavo , Pawlik Marek , Bradshaw Peter , Klein Bern
Published: August 2023

To support the transformation to clean low carbon technologies, there is a demand for critical metals such as nickel. Awaruite is a less common nickel-bearing mineral with unique properties and responses to mineral separation. This paper presents the findings of a flotation study to recover awaruite from the Baptiste ultramafic deposit, located in central British Columbia, Canada. Nickel recoveries of up to 65% at the rougher stage were obtained with xanthate as a collector at a pH level of 4.5. Awaruite flotation was shown to be highly dependent on the slurry pH. At weakly acidic conditions, the awaruite surface is activated through the dissolution of the passivation layer formed during grinding in alkaline conditions. Desliming was shown to reduce the acid consumption required to maintain the pH, probably by removing the highly reactive serpentine slimes generated during grinding. Rougher, followed by cleaner stages of flotation, showed that a high-grade concentrate can be produced with up to 45% nickel, 1.3% cobalt, 0.7% copper and negligible concentrations of penalty elements, such as arsenic, lead, and selenium, among others. A nickel flotation concentrate from an awaruite deposit is a promising feedstock for not only stainless-steel production but also for clean energy technologies.
Mild Conditions Method to Remediate Hazardous Jarosite and Its Application as Adsorbent of Arsenic(V) and Water
Authors: López-Martínez Arely Monserrat , Gamero-Melo Prócoro
Published: August 2023

The environmental risks of industrial jarosite (JAR) were mainly attributed to its average particle size (8.6 µm) and its content of leachable heavy metals such as cadmium (Cd, 64.2 mg L−1), lead (Pb, 4.16 mg L−1), and arsenic (As, 0.27 mg L−1). In this study, various methods were reported to eliminate the leachable elements contained in JAR without collapsing the crystalline structure: acid leaching, ionic exchange, and extended remediation. The effect of pH (2–10), temperature (20–175 °C), and time (<30 min) on the leaching of Cd, Pb, and As were studied. The ultrasound-assisted leaching process (at <60 °C and for 12 min) using a 0.19 M H2SO4 solution allowed to obtain a reduction of leachable Cd (99.2 wt. %), Pb (94.2 wt. %), and As (98.1 wt. %). Although the H2SO4 remediated jarosite, for example, still had a content of Pb, Cd, As, and Mn of 9.25, 0.91, 3.89, and 2.41 g kg−1, respectively, these metallic compounds were insoluble in the pH interval of 2 to 10. The jarosite obtained using acid leaching, JAR2L, had the highest adsorption capacity of As(V) (Qmax = 7.55 g kg−1), while the jarosite obtained using extended remediation had the highest water adsorption capacity (165 mL kg−1). The JAR can be remediated using acid ultrasound-assisted leaching and it can be applied in formulating strategic materials for the chemical industry.
Nutritional Composition, Fatty Acids Profile, Mineral Content, Antioxidant Activity and Acute Toxicity of the Flesh of Helix aspersa Müller
Authors: Aouji Marouane , Imtara Hamada , Rkhaila Amine , Bouhaddioui Bouchra , Alahdab Ahmad , Parvez Mohammad , Saleh Alzahrani Mohamed , Aicha Lrhorfi Lalla , Bengueddour Rachid
Published: August 2023

Humans consume snail flesh as part of their diet. To assess its nutritional value and toxicity, chemical analyses were conducted to confirm the presence of protein, total and reduced carbohydrates, fat, fatty acid composition and mineral components. Furthermore, an acute toxicity study was carried out to determine the safety of Helix aspersa Müller snail flesh. H. aspersa Müller snail flesh exhibits a high nutritional content, a good ω3/ω6 ratio and higher levels of unsaturated fatty acids. Various minerals have been found in the flesh of H. aspersa Müller. Around 76.91 kcal, or 3.84% of the energy of a daily meal of 2000 kcal, are present in 100 g of this flesh. The evaluation of the antioxidant capacity indicated that the flesh’s extracts contained a large quantity of antioxidant biomolecules. Administration of the aqueous extract of H. aspersa Müller flesh didn’t cause death in laboratory rats, indicating that the lethal dose 50 is greater than 2000 mg·kg−1 body weight. The consumption of the flesh of H. aspersa Müller is highly recommended for human consumption due to its high concentration of nutrients and essential elements, as well as unsaturated fats, and due to its safety.
Ganoderma lucidum Mycelia Mass and Bioactive Compounds Production through Grape Pomace and Cheese Whey Valorization
Authors: Kachrimanidou Vasiliki , Papadaki Aikaterini , Papapostolou Harris , Alexandri Maria , Gonou-Zagou Zacharoula , Kopsahelis Nikolaos
Published: August 2023

Numerous compounds obtained from the medicinal mushroom Ganoderma lucidum have evidenced renowned bioactive characteristics. Controlled fermentation to generate fungal mycelia confers several advantages, specifically when the valorization of agro-industrial streams as fermentation feedstocks is included. Submerged fermentation of a newly isolated Greek strain of G. lucidum was performed using conventional synthetic media and, also, grape pomace extract (GPE) and cheese whey permeate (CWP) under static and shaking conditions. Under shaking conditions, maximum biomass with GPE and supplementation with organic nitrogen reached 17.8 g/L. The addition of an elicitor in CWP resulted in a significant improvement in biomass production that exceeded synthetic media. Overall, agitation demonstrated a positive impact on biomass productivity and, therefore, on process optimization. Crude intracellular and extracellular polysaccharides were extracted and evaluated regarding antioxidant activity and polysaccharide and protein content. FTIR analysis confirmed the preliminary chemical characterization of the crude extracts. This study introduces the design of a bioprocessing scenario to utilize food industry by-products as onset feedstocks for fungal bioconversions to obtain potential bioactive molecules within the concept of bioeconomy.
Porous Metal–Organic Frameworks for Light Hydrocarbon Separation
Authors: Gao Xiang , Yan Wen-Hui , Hu Bo-Yang , Huang Yu-Xin , Zheng Shi-Mei
Published: August 2023

The separation of light hydrocarbon compounds is an important process in the chemical industry. Currently, its separation methods mainly include distillation, membrane separation, and physical adsorption. However, these traditional methods or materials have some drawbacks and disadvantages, such as expensive equipment costs and high energy consumption, poor selectivity, low separation ratios, and separation efficiencies. Therefore, it is important to develop novel separation materials for light hydrocarbon separation. As a new type of organic–inorganic hybrid crystalline material, metal–organic frameworks (MOFs) are promising materials for light hydrocarbon separation due to their designability of structure and easy modulation of function. This review provides an overview of recent advances in the design, synthesis, and application of MOFs for light hydrocarbon separation in recent years, with a focus on the separation of alkane, alkene, and alkyne. We discuss strategies for improving the adsorption selectivity and capacity of MOFs, including pore size limitation, physical adsorption, and chemisorption. In addition, we discuss the advantages/disadvantages, challenges, and prospects of MOFs in the separation of light hydrocarbon.
Recovery of Precious Metals: A Promising Process Using Supercritical Carbon Dioxide and CO2-Soluble Complexing Polymers for Palladium Extraction from Supported Catalysts
Authors: Ruiu Andrea , Li W. S. Jennifer , Senila Marin , Bouilhac Cécile , Foix Dominique , Bauer-Siebenlist Bernhard , Seaudeau-Pirouley Karine , Jänisch Thorsten , Böringer Sarah , Lacroix-Desmazes Patrick
Published: August 2023

Precious metals such as palladium (Pd) have many applications, ranging from automotive catalysts to fine chemistry. Platinum group metals are, thus, in massive demand for industrial applications, even though they are relatively rare and belong to the list of critical materials for many countries. The result is an explosion of their price. The recovery of Pd from spent catalysts and, more generally, the development of a circular economy process around Pd, becomes essential for both economic and environmental reasons. To this aim, we propose a sustainable process based on the use of supercritical CO2 (i.e., a green solvent) operated in mild conditions of pressure and temperature (p = 25 MPa, T = 313 K). Note that the range of CO2 pressures commonly used for extraction is going from 15 to 100 MPa, while temperatures typically vary from 308 to 423 K. A pressure of 25 MPa and a temperature of 313 K can, therefore, be viewed as mild conditions. CO2-soluble copolymers bearing complexing groups, such as pyridine, triphenylphosphine, or acetylacetate, were added to the supercritical fluid to extract the Pd from the catalyst. Two supported catalysts were tested: a pristine aluminosilicate-supported catalyst (Cat D) and a spent alumina supported-catalyst (Cat A). An extraction conversion of up to more than 70% was achieved in the presence of the pyridine-containing copolymer. The recovery of the Pd from the polymer was possible after extraction, and the technological and economical assessment of the process was considered.
Rapid Chemical Profiling of Filipendula ulmaria Using CPC Fractionation, 2-D Mapping of 13C NMR Data, and High-Resolution LC–MS
Authors: Pannakal Steve , Eilstein Joan , Hubert Jane , Kotland Alexis , Prasad Arpita , Gueguiniat-Prevot Amelie , Juchaux Franck , Beaumard Floriane , Seru Ganapaty , John Sherluck , Roy Dhimoy
Published: August 2023

Filipendula ulmaria, commonly known as meadowsweet, is a wild herbaceous flowering plant that is widely distributed in Europe. A range of salicylic acid derivatives and flavonol glycosides have been previously associated with the antirheumatic and diuretic properties of F. ulmaria. In the present work, a hydroalcoholic extract from F. ulmaria aerial parts was extensively profiled using an efficient NMR-based dereplication strategy. The approach involves the fractionation of the crude extract by centrifugal partition chromatography (CPC), 13C NMR analysis of the fractions, 2D-cluster mapping of the entire NMR dataset, and, finally, structure elucidation using a natural metabolite database, validated by 2D NMR data interpretation and liquid chromatography coupled with mass spectrometry. The chemodiversity of the aerial parts was extensive, with 28 compounds unambiguously identified, spanning various biosynthetic classes. The F. ulmaria extract and CPC fractions were screened for their potential to enhance skin epidermal barrier function and skin renewal properties using in vitro assays performed on Normal Human Epidermal Keratinocytes. Fractions containing quercetin, kaempferol glycosides, ursolic acid, pomolic acid, naringenin, β-sitosterol, and Tellimagrandins I and II were found to upregulate genes related to skin barrier function, epidermal renewal, and stress responses. This research is significant as it could provide a natural solution for improving hydration and skin renewal properties.
Single-Molecule Magnet Properties in 3d4f Heterobimetallic Iron and Dysprosium Complexes Involving Hydrazone Ligand
Authors: Lefeuvre Bertrand , Guizouarn Thierry , Dorcet Vincent , Cordier Marie , Pointillart Fabrice
Published: August 2023

The reaction between the ((E)-N′-(2-hydroxy-3-methoxybenzylidene)pyrazine-2-carbohydrazide) (H2opch) ligand and the metallo-precursor [Dy(hfac)3]·2H2O led to the formation of an homometallic coordination complex with the formula [Dy2(hfac)3(H2O)(Hopch)2][Dy(hfac)4] (1). In presence of both [Dy(hfac)3] 2H2O and the Fe(II) salt, the heterobimetallic tetranuclear [FeDy3(hfac)8(H2O)2(opch)2] (2) was isolated, while the addition of the co-ligand 1,2-Bis(2-hydroxy-3-methoxybenzylidene) hydrazine (H2bmh) led to the formation of two heterobimetallic tetranuclear complexes with the formula [Fe3Dy(hfac)6(opch)2(H2bmh)] C6H14 (3) C6H14 and [Fe2Dy2(hfac)7(opch)2(H2bmh)] 0.5C7H16 (4) 0.5C7H16. Single crystal X-ray diffraction and dc magnetic investigation demonstrated that 3 and 4 involved the iron center in the +II and +III oxidation states. Dynamic magnetic measurements highlighted the single-molecule magnet behavior of 1 and 2 in a zero applied dc field primarily due to the ferromagnetic interactions taking place in these compounds.
Direct Polyphenol Attachment on the Surfaces of Magnetite Nanoparticles, Using Vitis vinifera, Vaccinium corymbosum, or Punica granatum
Authors: Matías-Reyes Ana E. , Alvarado-Noguez Margarita L. , Pérez-González Mario , Carbajal-Tinoco Mauricio D. , Estrada-Muñiz Elizabeth , Fuentes-García Jesús A. , Vega-Loyo Libia , Tomás Sergio A. , Goya Gerardo F. , Santoyo-Salazar Jaime
Published: August 2023

This study presents an alternative approach to directly synthesizing magnetite nanoparticles (MNPs) in the presence of Vitis vinifera, Vaccinium corymbosum, and Punica granatum derived from natural sources (grapes, blueberries, and pomegranates, respectively). A modified co-precipitation method that combines phytochemical techniques was developed to produce semispherical MNPs that range in size from 7.7 to 8.8 nm and are coated with a ~1.5 nm thick layer of polyphenols. The observed structure, composition, and surface properties of the MNPs@polyphenols demonstrated the dual functionality of the phenolic groups as both reducing agents and capping molecules that are bonding with Fe ions on the surfaces of the MNPs via –OH groups. Magnetic force microscopy images revealed the uniaxial orientation of single magnetic domains (SMDs) associated with the inverse spinel structure of the magnetite (Fe3O4). The samples’ inductive heating (H0 = 28.9 kA/m, f = 764 kHz), measured via the specific loss power (SLP) of the samples, yielded values of up to 187.2 W/g and showed the influence of the average particle size. A cell viability assessment was conducted via the MTT and NRu tests to estimate the metabolic and lysosomal activities of the MNPs@polyphenols in K562 (chronic myelogenous leukemia, ATCC) cells.
Electrodeposition Synthesis of Coral-like MnCo Selenide Binder-Free Electrodes for Aqueous Asymmetric Supercapacitors
Authors: Shao Siqi , Liu Song , Xue Changguo
Published: August 2023

Bimetallic selenide compounds show great potential as supercapacitor electrode materials in energy storage and conversion applications. In this work, a coral-like MnCo selenide was grown on nickel foam using a facile electrodeposition method to prepare binder-free supercapacitor electrodes. The heating temperature was varied to tune the morphology and crystal phase of these electrodes. Excellent electrochemical performance was achieved due to the unique coral-like, dendritic- dispersed structure and a bimetallic synergistic effect, including high specific capacitance (509 C g−1 at 1 A g−1) and outstanding cycling stability (94.3% capacity retention after 5000 cycles). Furthermore, an asymmetric supercapacitor assembled with MnCo selenide as the anode and active carbon as the cathode achieved a high specific energy of 46.2 Wh kg−1 at 800 W kg−1. The work demonstrates that the prepared coral-like MnCo selenide is a highly promising energy storage material.
In Situ Fabrication of CdS/Cd(OH)2 for Effective Visible Light-Driven Photocatalysis
Authors: Chen Ran , Qian Liping , Xu Shengyou , Wan Shunli , Ma Minghai , Zhang Lei , Jiang Runren
Published: August 2023

Photocatalytic hydrogen production is a promising technology that can generate renewable energy. However, light absorption and fast electron transfer are two main challenges that restrict the practical application of photocatalysis. Moreover, most of the composite photocatalysts that possess better photocatalytic performance are fabricated by various methods, many of which are complicated and in which, the key conditions are hard to control. Herein, we developed a simple method to prepare CdS/Cd(OH)2 samples via an in situ synthesis approach during the photocatalytic reaction process. The optimal hydrogen generation rate of CdS/Cd(OH)2 that could be obtained was 15.2 mmol·h−1·g−1, greater than that of CdS, which generates 2.6 mmol·h−1·g−1 under visible light irradiation. Meanwhile, the CdS-3 sample shows superior HER performance during recycling tests and exhibits relatively steady photocatalytic performance in the 10 h experiment. Expanded absorption of visible light, decreased recombination possibility for photo-induced carriers and a more negative conduction band position are mainly responsible for the enhanced photocatalytic hydrogen evolution performance. Photo-induced electrons will be motivated to the conduction band of CdS under the irradiation of visible light and will further transfer to Cd(OH)2 to react with H+ to produce H2. The in situ-formed Cd(OH)2 could effectively facilitate the electron transfer and reduce the recombination possibility of photo-generated electron-hole pairs.
Engineering Materials and Devices for the Prevention, Diagnosis, and Treatment of COVID-19 and Infectious Diseases
Authors: Soto Jennifer , Linsley Chase , Song Yang , Chen Binru , Fang Jun , Neyyan Josephine , Davila Raul , Lee Brandon , Wu Benjamin , Li Song
Published: August 2023

Following the global spread of COVID-19, scientists and engineers have adapted technologies and developed new tools to aid in the fight against COVID-19. This review discusses various approaches to engineering biomaterials, devices, and therapeutics, especially at micro and nano levels, for the prevention, diagnosis, and treatment of infectious diseases, such as COVID-19, serving as a resource for scientists to identify specific tools that can be applicable for infectious-disease-related research, technology development, and treatment. From the design and production of equipment critical to first responders and patients using three-dimensional (3D) printing technology to point-of-care devices for rapid diagnosis, these technologies and tools have been essential to address current global needs for the prevention and detection of diseases. Moreover, advancements in organ-on-a-chip platforms provide a valuable platform to not only study infections and disease development in humans but also allow for the screening of more effective therapeutics. In addition, vaccines, the repurposing of approved drugs, biomaterials, drug delivery, and cell therapy are promising approaches for the prevention and treatment of infectious diseases. Following a comprehensive review of all these topics, we discuss unsolved problems and future directions.
Design, Synthesis, and Characterization of a Novel Blue-Green Long Afterglow BaYAl3O7:Eu2+, Nd3+ Phosphor and Its Anti-Counterfeiting Application
Authors: Wu Jiao , Liu Quanxiao , Gao Peng , Wang Jigang , Qi Yuansheng , Li Zhenjun , Li Junming , Jiang Tao
Published: August 2023

Herein, a series of novel long afterglow nanophosphors BaYAl3O7:Eu2+, Nd3+ was synthesized by the combustion method. The investigation encompassed the characterization of X-ray diffraction, morphology, chemical valence, elemental composition, and photoluminescence behavior of BaYAl3O7:Eu2+ and BaYAl3O7:Eu2+, Nd3+ nanoparticles. Under 365 nm excitation, BaYAl3O7:Eu2+ and BaYAl3O7:Eu2+, Nd3+ show emission bands centered at 497 nm and 492 nm, which are attributed to the 4f65d→4f7 transition of Eu2+ ions. The optimal samples of BaYAl3O7:0.03Eu2+ and BaYAl3O7:0.03Eu2+, 0.02Nd3+ have average fluorescence lifetimes of 850 ns and 1149 ns, respectively. The co-doping of Nd3+ ions as the trap centers produced long afterglow luminescence properties, and the afterglow time could reach up to 8 min. Furthermore, the fluorescent powder can be mixed with polyacrylic acid to prepare anti-counterfeiting inks; a clover pattern and snowflake pattern have been successfully printed using screen printing technology, proving its potential application in the field of anti-counterfeiting.
Microstructure and Oxygen Evolution Property of Prussian Blue Analogs Prepared by Mechanical Grinding
Authors: Meena Abhishek , Bathula Chinna , Hatshan Mohammad Rafe , Palem Ramasubba Reddy , Jana Atanu
Published: August 2023

Solvent-free mechanochemical synthesis of efficient and low-cost double perovskite (DP), like a cage of Prussian blue (PB) and PB analogs (PBAs), is a promising approach for different applications such as chemical sensing, energy storage, and conversion. Although the solvent-free mechanochemical grinding approach has been extensively used to create halide-based perovskites, no such reports have been made for cyanide-based double perovskites. Herein, an innovative solvent-free mechanochemical synthetic strategy is demonstrated for synthesizing Fe4[Fe(CN)6]3, Co3[Fe(CN)6]2, and Ni2[Fe(CN)6], where defect sites such as carbon–nitrogen vacancies are inherently introduced during the synthesis. Among all the synthesized PB analogs, the Ni analog manifests a considerable electrocatalytic oxygen evolution reaction (OER) with a low overpotential of 288 mV to obtain the current benchmark density of 20 mA cm−2. We hypothesize that incorporating defects, such as carbon–nitrogen vacancies, and synergistic effects contribute to high catalytic activity. Our findings pave the way for an easy and inexpensive large-scale production of earth-abundant non-toxic electrocatalysts with vacancy-mediated defects for oxygen evolution reaction.
Serum GDF-15 Levels in Patients with Parkinson’s Disease, Progressive Supranuclear Palsy, and Multiple System Atrophy
Authors: Miyaue Noriyuki , Yabe Hayato , Nagai Masahiro
Published: August 2023

Serum growth differentiation factor 15 (GDF-15) levels are elevated in patients with Parkinson’s disease (PD) and may help differentiate these patients from healthy individuals. We aimed to clarify whether serum GDF-15 levels can help differentiate PD from atypical parkinsonian syndromes and determine the association between serum GDF-15 levels and clinical parameters. We prospectively enrolled 46, 15, and 12 patients with PD, progressive supranuclear palsy (PSP), and multiple system atrophy (MSA), respectively. The serum GDF-15 level in patients with PD (1394.67 ± 558.46 pg/mL) did not differ significantly from that in patients with PSP (1491.27 ± 620.78 pg/mL; p = 0.573) but was significantly higher than that in patients with MSA (978.42 ± 334.66 pg/mL; p = 0.017). Serum GDF-15 levels were positively correlated with age in patients with PD (r = 0.458; p = 0.001); PSP (r = 0.565; p = 0.028); and MSA (r = 0.708; p = 0.010). After accounting for age differences, serum GDF-15 levels did not differ significantly between patients with PD and MSA (p = 0.114). Thus, age has a strong influence on serum GDF-15 levels, which may not differ significantly between patients with PD and atypical parkinsonian syndromes such as PSP and MSA.
Liver Disease Undernutrition Screening Tool Questionnaire Predicts Decompensation and Mortality in Cirrhotic Outpatients with Portal Hypertension
Authors: Casas-Deza Diego , Bernal-Monterde Vanesa , Betoré-Glaria Elena , Julián-Gomara Ana Belén , Yagüe-Caballero Carmen , Sanz-París Alejandro , Fernández-Bonilla Eva María , Fuentes-Olmo Javier , Arbones-Mainar Jose M.
Published: August 2023

Background: Numerous scores are designed to predict outcomes of patients with liver cirrhosis. Our study aimed to evaluate the ability of the Liver Disease Undernutrition Screening Tool (LDUST) in predicting mortality and decompensation in outpatients with clinically significant portal hypertension (CSPH). We hypothesized that LDUST could help identify patients in need of nutritional supplementation and intervention. Methods: A prospective study of 57 CSPH patients (36.8% female, mean age: 63.5 ± 9.9 years) with a median follow-up of 41 months was conducted. Baseline liver function, nutrition, and sarcopenia were assessed, alongside LDUST. During follow-up, the occurrence of liver decompensation, hospital admission, need for emergency care, and mortality were evaluated. Results: A total of 56.1% of patients were Child A, and the most frequent etiology was alcohol (50.9%). Malnutrition risk according to LDUST raised mortality (HR: 25.96 (1.47–456.78)), decompensation (HR 9.78 (2.08–45.89)), and admission (HR 4.86 (1.09–21.61)) risks in multivariate Cox analysis. Combining LDUST with Child and MELD scores improved their decompensation prediction (0.936 vs. 0.811 and 0.866 vs. 0.700). Conclusions: The LDUST has a solid ability to predict complications in cirrhosis outpatients with CSPH, and its integration with Child and MELD models enhances their predictive power. LDUST implementation could identify individuals necessitating early nutritional support.
Systematic Review of Management of Moderate Wasting in Children over 6 Months of Age
Authors: Padhani Zahra A. , Cichon Bernardette , Das Jai K. , Salam Rehana A. , Stobaugh Heather C. , Mughal Muzna , Rutishauser-Perera Alexandra , Black Robert E. , Bhutta Zulfiqar A.
Published: August 2023

The effective management of the 33 million children with moderate acute malnutrition (MAM) is key to reducing childhood morbidity and mortality. In this review, we aim to evaluate the effectiveness of specially formulated foods (SFFs) compared to non-food-based approaches to manage MAM in children >6 months old. We conducted a search on ten databases until 23 August 2021 and included five studies, covering 3387 participants. Meta-analysis of four studies comparing SFFs to counselling or standard of care showed that SFFs likely increase recovery rate, reduce non-response, and may improve weight-for-height z-score, weight-for-age z-score and time to recovery, but have little or no effect on MUAC gain. One study on a multicomponent intervention (SFFs, antibiotics and counselling provided to high-risk MAM) compared to counselling only was reported narratively. The intervention may increase weight gain after 24 weeks but may have little or no effect on weight gain after 12 weeks and on non-response and mortality after 12 and 24 weeks of enrollment. The effect of this intervention on recovery was uncertain. In conclusion, SFFs may be beneficial for children with moderate wasting in humanitarian contexts. Programmatic recommendations should consider context and cost-effectiveness.
Micronutrient Status and Other Correlates of Hemoglobin among Children with Stunting: A Cross-Sectional Study in Uganda
Authors: Mutumba Rolland , Mbabazi Joseph , Pesu Hannah , Greibe Eva , Olsen Mette F. , Briend André , Mølgaard Christian , Ritz Christian , Mupere Ezekiel , Filteau Suzanne , Friis Henrik , Grenov Benedikte
Published: August 2023

In low-income countries, undernutrition and infections play a major role in childhood anemia. Stunted children may be at particular risk of anemia. In a cross-sectional study nested in a nutrition trial among 12–59-month-old stunted children in eastern Uganda, we measured hemoglobin (Hb) and markers of iron, cobalamin, folate and vitamin A status. We assessed low micronutrient status, socio-demography, stunting severity, inflammation and malaria as correlates of Hb and anemia using linear and logistic regression analyses, respectively. Of 750 stunted children, the mean ± SD age was 32.0 ± 11.7 months and 55% (n = 412) were male. The mean Hb was 104 ± 15 g/L and 65% had anemia, Hb < 110 g/L. In a multivariable model with age, sex and inflammation, the following were associated with lower Hb: serum ferritin < 12 µg/L (−5.6 g/L, 95% CI: −8.6; −2.6), transferrin receptors > 8.3 mg/L (−6.2 g/L, 95% CI: −8.4; −4.0), plasma folate <20 nmol/L (−4.6 g/L, 95% CI: −8.1;−1.1), cobalamin < 222 pmol/L (−3.0 g/L, 95% CI: −5.4; −0.7) and serum retinol-binding protein < 0.7 µmol/L (−2.0 g/L, 95% CI: −4.1; 0.2). In addition, severe stunting, inflammation and malaria were negative correlates. Anemia is common among stunted children in eastern Uganda; micronutrient deficiencies, inflammation and malaria are associated with low Hb.
A Randomized, Double-Blind, Placebo-Controlled Decentralized Trial to Assess Sleep, Health Outcomes, and Overall Well-Being in Healthy Adults Reporting Disturbed Sleep, Taking a Melatonin-Free Supplement
Authors: Kolobaric Antonija , Hewlings Susan J. , Bryant Corey , Colwell Christopher S. , R. D’Adamo Christopher , Rosner Bernard , Chen Jeff , Pauli Emily K.
Published: August 2023

Inadequate sleep is a global health concern. Sleep is multidimensional and complex; new multi-ingredient agents are needed. This study assessed the comparative effects of two multi-ingredient supplements on sleep relative to placebo. Adults (N = 620) seeking better sleep were randomly assigned to receive one of three study products. Sleep A (contained lower (0.35 mg THC and higher levels of botanicals (75 mg each hops oil and valerian oil), Sleep B (contained higher THC (0.85 mg) and lower botanicals (20 mg each hops oil and valerian oil) or placebo) for 4 weeks. Sleep disturbance was assessed at baseline and weekly using NIH’s Patient-Reported Outcomes Measurement Information System (PROMIS™) Sleep Disturbance SF 8A survey. Anxiety, stress, pain, and well-being were assessed using validated measures at baseline and weekly. A linear mixed-effects regression model was used to assess the change in health outcome score between active product groups and the placebo. There was a significant difference in sleep disturbance, anxiety, stress, and well-being between Sleep A and placebo. There was no significant difference in any health parameter between Sleep B and placebo. Side effects were mild or moderate. There were no significant differences in the frequency of side effects between the study groups. A botanical blend containing a low concentration of THC improved sleep disturbance, anxiety, stress, and well-being in healthy individuals that reported better sleep as a primary health concern.
Hydration Status of Geriatric Patients Is Associated with Changes in Plasma Proteome, Especially in Proteins Involved in Coagulation
Authors: Hoen Laura , Pfeffer Daniel , Schmidt Johannes R. , Kraft Johannes , Hildebrand Janosch , Kalkhof Stefan
Published: August 2023

Due to multifactorial reasons, such as decreased thirst and decreased total body water, elderly patients are vulnerable to dehydration. The study aims to investigate whether moderate dehydration or hyperhydration affects the blood proteome. Blood samples, medication, and bioelectrical impedance analysis (BIA) details were collected from 131 geriatric patients (77 women and 54 men aged 81.1 ± 7.2 years). Based on an evaluation by Bioelectrical Impedance Vector Analyses (BIVAs) of this cohort, for each hydration status (dehydrated, hyperhydrated, and control), five appropriate blood plasma samples for both males and females were analyzed by liquid chromatography–mass spectrometry (LC-MS). Overall, 262 proteins for female patients and 293 proteins for male patients could be quantified. A total of 38 proteins had significantly different abundance, showing that hydration status does indeed affect the plasma proteome. Protein enrichment analysis of the affected proteins revealed “Wound Healing” and “Keratinization” as the two main biological processes being dysregulated. Proteins involved in clot formation are especially affected by hydration status.
Diet Outcomes from a Randomized Controlled Trial Assessing a Parenting Intervention Simultaneously Targeting Healthy Eating and Substance Use Prevention among Hispanic Middle-School Adolescents
Authors: Vega-López Sonia , Ayers Stephanie , Gonzalvez Anaid , Campos Ana Paola , Marsiglia Flavio F. , Bruening Meg , Rankin Lela , Vega Luna Beatriz , Biggs Elizabeth , Perilla Alex
Published: August 2023

Parents play a significant role in adolescent health behaviors; however, few nutrition interventions for Hispanic adolescents involve parents. This study assessed the effects of a 10-week parenting intervention simultaneously targeting nutrition and substance use prevention. Hispanic parent/6th–8th-grade adolescent dyads (n = 239) were randomized to Families Preparing the New Generation Plus (FPNG+; nutrition/substance use prevention), FPNG (substance use prevention only), or Realizing the American Dream (RAD; academic success control). Surveys assessed diet, alcohol use, substance use intentions, and substance use norms at baseline (T1), immediately post-intervention (T2), and at 16 weeks post-intervention (T3). Latent change modeling assessed diet changes; adolescent substance use outcomes were assessed using effect sizes. Among adolescents, those in FPNG+ increased fruit (+0.32 cup equivalents, p = 0.022) and fiber intake (+1.06 g, p = 0.048) and did not change added sugars intake at T2; those in FPNG and RAD reduced their intake of fruit and fiber (p < 0.05 for both). FPNG+ parents marginally increased fruit/vegetable intake (+0.17 cup equivalents, p = 0.054) and increased whole grains intake (+0.25-ounce equivalents, p < 0.05), in contrast to the reduction among RAD and FPNG parents (p < 0.05). Reductions in added sugar intake at T2 were greater among FPNG and FPNG+ parents relative to RAD parents (p < 0.05). FPNG+ and FPNG had comparable substance use outcomes (i.e., both had lower alcohol use and intentions to use substances relative to RAD). Engaging parents in a nutrition and substance use prevention parenting intervention yielded positive changes in dietary intake and maintained substance use prevention outcomes among their adolescent children.
Breakfast Consumption and Quality of Macro- and Micronutrient Intake in Indonesia: A Study from the Indonesian Food Barometer
Authors: Khusun Helda , Anggraini Roselynne , Februhartanty Judhiastuty , Mognard Elise , Fauzia Khalida , Maulida Nursyifa Rahma , Linda Ony , Poulain Jean-Pierre
Published: August 2023

Breakfast is an important meal that has been shown to have a positive effect on health. The current study aimed to assess the patterns of breakfast consumption among adult Indonesians and to estimate the contribution of breakfast to their nutrient intake and dietary quality. The study used 24-h recall data from the 2018 Indonesian Food Barometer study to assess breakfast intake among 1333 adults aged 18 and above from six provinces in Indonesia. Diet quality was measured using the Nutrient Rich Food index (NRF) 9.3, and the nutritional profile of breakfast was compared across tertiles of NRF 9.3. In total, 5.2% of adults in Indonesia skipped breakfast. Breakfast contributed 26% to daily energy intakes and 22–28% to intakes of all reported nutrients, except for total sugar (12%), vitamin C (8%) and vitamin D (7%). With respect to daily requirements, breakfast contributed approximately 20% to energy, protein, fat and sodium requirements, 26% to saturated fat but <15% to the requirements for most micronutrients and only 5% for fiber. Among breakfast consumers, a higher NRF score was associated with higher daily intakes of protein, dietary fiber and micronutrients and lower intakes of sodium from breakfast. This study suggests that a balanced breakfast in Indonesia should aim to lower fat and saturated fat intake while increasing fiber, potassium, calcium and vitamin C and D intake. These findings could inform the development of nutrient-based guidelines for breakfast consumption in Indonesia.
In Vitro Antibody-Dependent Enhancement of SARS-CoV-2 Infection Could Be Abolished by Adding Human IgG
Authors: Wang Xun , Li Minghui , Lu Panpan , Li Chen , Zhao Chaoyue , Zhao Xiaoyu , Qiao Rui , Cui Yuchen , Chen Yanjia , Li Jiayan , Cai Guonan , Wang Pengfei
Published: August 2023

Evidence of antibody-dependent enhancement (ADE) of other viruses has raised concerns about the safety of SARS-CoV-2 vaccines and antibody therapeutics. In vitro studies have shown ADE of SARS-CoV-2 infection. In this study, we also found that vaccination/convalescent sera and some approved monoclonal antibodies can enhance SARS-CoV-2 infection of FcR-expressing B cells in vitro. However, the enhancement of SARS-CoV-2 infection can be prevented by blocking Fc–FcR interaction through the addition of human serum/IgG or the introduction of mutations in the Fc portion of the antibody. It should be noted that ADE activity observed on FcR-expressing cells in vitro may not necessarily reflect the situation in vivo; therefore, animal and clinical data should be included for ADE evaluation.