bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution of the Use of Corticosteroids for the Treatment of Hospitalised COVID-19 Patients in Spain between March and November 2020: SEMI-COVID National Registry
Authors: Balaz David , Wikman-Jorgensen Philip Erick , Galvañ Vicente Giner , Rubio-Rivas Manuel , de Miguel Campo Borja , López Mariam Noureddine , Caleya Juan Francisco López , Huelgas Ricardo Gómez , Fontán Paula María Pesqueira , Bailón Manuel Méndez , Fernández-Garcés Mar , Cruz Ana Fernández , García Gema María García , Rhyman Nicolás , Corral-Gudino Luis , Rodríguez-Mancheño Aquiles Lozano , De La Chica María Navarro , García Andrea Torregrosa , Alcalá José Nicolás , Jiménez Pablo Díaz , Trallero Leticia Esther Royo , Casanova Pere Comas , Núñez-Cortés Jesús Millán , Casas-Rojo José-Manuel ,   on behalf of the SEMI-COVID-19 Network
Published: October 2021

Objectives: Since the results of the RECOVERY trial, WHO recommendations about the use of corticosteroids (CTs) in COVID-19 have changed. The aim of the study is to analyse the evolutive use of CTs in Spain during the pandemic to assess the potential influence of new recommendations. Material and methods: A retrospective, descriptive, and observational study was conducted on adults hospitalised due to COVID-19 in Spain who were included in the SEMI-COVID-19 Registry from March to November 2020. Results: CTs were used in 6053 (36.21%) of the included patients. The patients were older (mean (SD)) (69.6 (14.6) vs. 66.0 (16.8) years; p < 0.001), with hypertension (57.0% vs. 47.7%; p < 0.001), obesity (26.4% vs. 19.3%; p < 0.0001), and multimorbidity prevalence (20.6% vs. 16.1%; p < 0.001). These patients had higher values (mean (95% CI)) of C-reactive protein (CRP) (86 (32.7–160) vs. 49.3 (16–109) mg/dL; p < 0.001), ferritin (791 (393–1534) vs. 470 (236–996) µg/dL; p < 0.001), D dimer (750 (430–1400) vs. 617 (345–1180) µg/dL; p < 0.001), and lower Sp02/Fi02 (266 (91.1) vs. 301 (101); p < 0.001). Since June 2020, there was an increment in the use of CTs (March vs. September; p < 0.001). Overall, 20% did not receive steroids, and 40% received less than 200 mg accumulated prednisone equivalent dose (APED). Severe patients are treated with higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%. Conclusions: Patients with greater comorbidity, severity, and inflammatory markers were those treated with CTs. In severe patients, there is a trend towards the use of higher doses. The mortality benefit was observed in patients with oxygen saturation </=90%.
Cardiometabolic Disorders and the Risk of Critical COVID-19 as Compared to Influenza Pneumonia
Authors: Fayol Antoine , Livrozet Marine , Pereira Héléna , Diehl Jean-Luc , Lebeaux David , Arlet Jean-Benoit , Cholley Bernard , Carette Claire , Carves Jean-Baptiste , Czernichow Sébastien , Hauw Caroline , Hamada Sophie-Rym , Jannot Anne-Sophie , Volle Geoffroy , Masurkar Nihar , Mirault Tristan , Planquette Benjamin , Sanchez Olivier , Châtellier Gilles , Azizi Michel , Hulot Jean-Sébastien
Published: October 2021

We aimed to compare the influence of cardiometabolic disorders on the incidence of severe COVID-19 vs. non-COVID pneumonia. We included all consecutive patients admitted with SARS-CoV-2-positive pneumonia between 12 March 2020 and 1 April 2020 and compared them to patients with influenza pneumonia hospitalized between December 2017 and December 2019 at the same tertiary hospital in Paris. Patients with COVID-19 were significantly younger and more frequently male. In the analysis adjusted for age and sex, patients with COVID-19 were more likely to be obese (adjOR: 2.25; 95% CI 1.24–4.09; p = 0.0076) and receive diuretics (adjOR: 2.13; 95% CI 1.12–4.03; p = 0.021) but were less likely to be smokers (adjOR: 0.40; 95% CI 0.24–0.64; p = 0.0002), have COPD (adjOR: 0.25; 95% CI 0.11–0.56; p = 0.0008), or have a previous or active cancer diagnosis (adjOR: 0.54, 95% CI 0.32–0.91; p = 0.020). The rate of ICU admission was significantly higher in patients with COVID-19 (32.4% vs. 5.2% p < 0.0001). Obesity was significantly associated with the risk of direct ICU admission in patients with COVID-19 but not in patients with influenza pneumonia. Likewise, pre-existing hypertension was significantly associated with mortality in patients with COVID-19 but not in patients with influenza pneumonia. Cardiometabolic disorders differentially influenced the risk of presenting with severe COVID-19 or influenza pneumonia.
Fibrosis Burden of Missed and Added Populations According to the New Definition of Metabolic Dysfunction-Associated Fatty Liver
Authors: Park Huiyul , Yoon Eileen L. , Kim Mimi , Kim Jung-Hwan , Cho Seon , Jun Dae Won , Nah Eun-Hee
Published: October 2021

Recently, the classification of fatty liver and the definition for non-alcoholic fatty liver disease (NAFLD) have been challenged. Herein, we aim to evaluate the burden of hepatic fibrosis in the missed and added populations following the proposal of the new definition of metabolic dysfunction-associated fatty liver (MAFLD) in a health check-up cohort. A total of 6775 subjects underwent both magnetic resonance elastography (MRE) and an abdominal ultrasound at 13 nationwide health check-up centers in Korea. Significant and advanced hepatic fibrosis was defined as ≥3.0 kPa and ≥3.6 kPa in the MRE test, respectively. The prevalence of sonographic fatty liver (FL) was 47.4%. Among the subjects with sonographic FL, 77.3% and 94% are compatible with NAFLD and with the new MAFLD definitions, respectively. Moreover, 72% of FL cases belong to both the NAFLD and MAFLD definitions, whereas 1.4% is compatible with neither. The population compatible with the MAFLD definition has the following coexisting liver diseases: alcohol-related (71.9%), hepatitis B (23.9%), hepatitis C (0.4%), and both alcohol and viral hepatitis (2.8%). The prevalence of significant and advanced hepatic fibrosis is considerable in the MAFLD-only group. However, the prevalence of significant and advanced hepatic fibrosis is similar in the NAFLD-only group, and neither the NAFLD nor MAFLD group compared to healthy controls. The added population (MAFLD-only group), according to the new MAFLD definition, has a higher metabolic and fibrosis burden when compared to those in the missed population (NAFLD-only group).
Why the Term MINOCA Does Not Provide Conceptual Clarity for Actionable Decision-Making in Patients with Myocardial Infarction with No Obstructive Coronary Artery Disease
Authors: Pelliccia Francesco , Marzilli Mario , Boden William E. , Camici Paolo G.
Published: October 2021

When acute myocardial injury is found in a clinical setting suggestive of myocardial ischemia, the event is labeled as acute myocardial infarction (MI), and the absence of ≥50% coronary stenosis at angiography or greater leads to the working diagnosis of myocardial infarction with non-obstructed coronary arteries (MINOCA). Determining the mechanism of MINOCA and excluding other possible causes for cardiac troponin elevation has notable implications for tailoring secondary prevention measures aimed at improving the overall prognosis of acute MI. The aim of this review is to increase the awareness that establishing the underlying cause of a MINOCA is possible in the vast majority of cases, and that the proper classification of any MI should be pursued. The initial diagnosis of MINOCA can be confirmed or ruled out based on the results of subsequent investigations. Indeed, a comprehensive clinical evaluation at the time of presentation, followed by a dedicated diagnostic work-up, might lead to the identification of the pathophysiologic abnormality leading to MI in almost all cases initially labeled as MINOCA. When a specific cause of acute MI is identified, cardiologists are urged to transition from the “all-inclusive” term “MINOCA” to the proper classification of any MI, as evidence now exists that MINOCA does not provide conceptual clarity for actionable decision-making in MI with angiographically normal coronary arteries.
The Microbiota-Gut-Brain Axis as a Key to Neuropsychiatric Disorders: A Mini Review
Authors: Stopińska Katarzyna , Radziwoń-Zaleska Maria , Domitrz Izabela
Published: October 2021

The central nervous system (CNS) is closely related to the gastrointestinal tract, mainly through regulating its function and homeostasis. Simultaneously, the gut flora affects the CNS and plays an essential role in the pathogenesis of neurologic and neuropsychological disorders such as Parkinson’s and Alzheimer’s disease, multiple sclerosis, amyotrophic lateral sclerosis or autism spectrum disorder. The population of gut microorganisms contains more than one billion bacteria. The most common are six phyla: Proteobacteria, Actinomyces, Verucomicrobia, Fusobacteria, and dominant Bacteroides with Firmicutes. The microbiota–gut–brain axis is a bidirectional nervous, endocrine, and immune communication between these two organs. They are connected through a variety of pathways, including the vagus nerve, the immune system, microbial metabolites such as short-chain fatty acids (SCFAs), the enteric nervous system, and hormones. Age, diet, antibiotics influence the balance of gut microorganisms and probably lead to the development of neurodegenerative disorders. In this article, a review is presented and discussed, with a specific focus on the changes of gut microbiota, gut–brain axis, related disorders, and the factors that influence gut imbalance.
Troglitazone-Induced PRODH/POX-Dependent Apoptosis Occurs in the Absence of Estradiol or ERβ in ER-Negative Breast Cancer Cells
Authors: Lewoniewska Sylwia , Oscilowska Ilona , Huynh Thi Yen Ly , Prokop Izabela , Baszanowska Weronika , Bielawska Katarzyna , Palka Jerzy
Published: October 2021

The impact of estradiol on troglitazone (TGZ)-induced proline dehydrogenase/proline oxidase (PRODH/POX)-dependent apoptosis was studied in wild-type and PRODH/POX-silenced estrogen receptor (ER) dependent MCF-7 cells and ER-independent MDA-MB-231 cells. DNA and collagen biosynthesis were determined by radiometric method, prolidase activity evaluated by colorimetric method, ROS production was measured by fluorescence assay. Protein expression was determined by Western blot and proline concentration by LC/MS analysis. PRODH/POX degrades proline yielding reactive oxygen species (ROS). Estrogens stimulate collagen biosynthesis utilizing free proline and limiting its availability for PRODH/POX-dependent apoptosis. TGZ cytotoxicity was highly pronounced in wild-type MDA-MB-231 cells cultured in medium without estradiol or in the cells cultured in medium with estradiol but deprived of ERβ (by ICI-dependent degradation), while in PRODH/POX-silenced cells the process was not affected. The TGZ cytotoxicity was accompanied by increase in PRODH/POX expression, ROS production, expression of cleaved caspase-3, caspase-9 and PARP, inhibition of collagen biosynthesis, prolidase activity and decrease in intracellular proline concentration. The phenomena were not observed in PRODH/POX-silenced cells. The data suggest that TGZ-induced apoptosis in MDA-MB-231 cells cultured in medium without estradiol or deprived of ERβ is mediated by PRODH/POX and the process is facilitated by proline availability for PRODH/POX by TGZ-dependent inhibition of collagen biosynthesis. It suggests that combined TGZ and antiestrogen treatment could be considered in experimental therapy of estrogen receptor negative breast cancers.
Sports Activity and Patient-Related Outcomes after Cementless Total Hip Arthroplasty in Patients Younger than 40 Years
Authors: Navas Luis , Faller Jasmin , Schmidt Sebastian , Streit Marcus , Hauschild Matthias , Zimmerer Alexander
Published: October 2021

Background: The management of degenerative hip diseases in young patients remains a challenge. Despite the improvement of hip-preserving procedures, total hip arthroplasty (THA) may be required in some instances. In addition, young patients undergoing THA have high expectations concerning their postoperative level of activity. Purpose: (1) to define the sports activity level and the return to sports after THA, (2) to describe the modification or initiation of new sports disciplines, and (3) to report the clinically meaningful outcomes after THA in patients younger than 40 years. Methods: A total of 36 patients (40 hips) were prospectively analyzed at a midterm follow-up of 3.9 years. The modified Harris Hip Score (mHHS); the Visual Analog Scale (VAS) for pain; the University of California, Los Angeles (UCLA) activity scale; and sports and recreational activity levels were assessed via questionnaire. The minimal clinically important difference (MCID) was determined by calculating half of the standard deviation, and the substantial clinical benefit (SCB) as well as patient acceptable symptomatic state (PASS), were calculated by the anchor method for the mHHS. Results: At the final follow-up, there was a significant improvement in mHHS (34.1 to 92.6; p < 0.0001), UCLA (3.2 to 7.6; p < 0.0001), and VAS for pain (8 to 1; p < 0.0001). More patients were active in sports at follow-up than before surgery (44% to 92%, p < 0.0001). In addition, the duration and frequency of sports activities showed a significant increase (p < 0.0001). The MCID, SCB and PASS for mHHS were 89% and 58%, respectively. No revision surgery had to be performed. Conclusion: This study showed that a large proportion of patients under 40 years of age who underwent THA increased their physical activity. Eighty-six percent of the patients were highly active, with a UCLA score ≥ 7. Furthermore, the reported MCID, SCB, and PASS for mHHS were achieved by more than 80% of patients.
RRM1 Expression as a Prognostic Biomarker for Unresectable or Recurrent Biliary Tract Cancer Treated with Gemcitabine plus Cisplatin
Authors: Chun Jung Won , Lee Boyoung , Park Weon Seo , Han Nayoung , Hong Eun Kyung , Park Eun Young , Han Sung Sik , Park Sang-Jae , Kim Tae Hyun , Lee Woo Jin , Woo Sang Myung
Published: October 2021

The combination of gemcitabine plus cisplatin (GP) is regarded as a first-line treatment for patients with unresectable or recurrent biliary tract cancer (BTC). Several proteins including human equilibrative nucleoside transporter-1 (hENT1), deoxycytidine kinase (DCK), cytidine deaminase (CDA), and ribonucleotide reductase subunit 1 (RRM1) are known to be involved in gemcitabine uptake and metabolism. This study was aimed to identify the predictive and prognostic values of these biomarkers in patients who treated with GP for advanced BTC. Tumor samples were obtained from 34 patients with unresectable or recurrent BTC who were treated with GP between August 2015 and February 2018. Intratumoral expression of hENT1, DCK, CDA and RRM1 was determined by immunohistochemistry and analyzed for association with chemotherapy response, progression-free survival (PFS) and overall survival (OS). Median OS was significantly longer in the RRM1-negative group than in the RRM1-positive (9.9 months vs. 5.9 months, p = 0.037). Multivariate adjustment analyses also demonstrated RRM1 expression as an independent prognostic factor for OS in patients treated with GP chemotherapy. Increased intratumoral expression of RRM1 on immunohistochemical staining may be a biomarker predicting poor survival in patients with GP chemotherapy for advanced BTC. Large-scale well-predefined prospective research is needed to validate the utility of biomarkers in clinical practice.
Polymer Blends and Polymer Nanocomposites for Photovoltaic (PV) Cells and an Investigation of the Material Deposition Techniques in PV Cell Fabrication
Authors: Ntanovasilis George , Zaverdas Ioannis , Ahmed Tarig , Markoulidis Foivos , Lekakou Constantina
Published: October 2021

Polymer photovoltaics (PV) offer the advantage of low-cost, mass-produced, flexible PV films, but they generally suffer from a low-power conversion efficiency (PCE) compared to silicon. This paper studies ITO/PEDOT:PSS/bulk heterojunction/Al PV cells, where two different bulk heterojunction blends are researched: P3HT/PC61BM and PCDTBT/PC70BM. The addition of multiwall carbon nanotubes (CNT) is explored as a conductive network to accelerate the electron transport and extraction to the outer aluminium current collector while reducing the chance of charge recombinations. Several layer deposition techniques are investigated: spin coating and casting, as well as techniques that would induce transverse orientation of polymer grains, including inkjet printing, electrophoresis and the application of a transverse AC field during annealing. Transverse orientation techniques produced architectures that would facilitate charge transport without recombinations, but it is recommended to avoid such techniques for the deposition of conductive PEDOT:PSS and CNT layers as they create a high surface roughness that leads to short circuiting. The best performing PV cell is the ITO/PEDOT:PSS/PCDTBT/PC70BM/CNT/Al structure with a PCE of 11%.
Numerical and Experimental Evaluation of Mechanical and Ring Stiffness Properties of Preconditioning Underground Glass Fiber Composite Pipes
Authors: Hassan Mohamed K. , Mohamed Ahmed F. , Khalil Khalil Abdelrazek , Abdellah Mohammed Y.
Published: October 2021

The mechanical and ring stiffness of glass fiber pipes are the most determining factors for their ability to perform their function, especially in a work environment with difficult and harmful conditions. Usually, these pipes serve in rough underground environments of desert and petroleum fields; therefore, they are subjected to multi-type deterioration and damage agents. In polymers and composite materials, corrosion is identified as the degradation in their properties. In this study, tension and compression tests were carried out before and after preconditioning in a corrosive agent for 60 full days to reveal corrosion influences. Moreover, the fracture toughness is measured using a standard single edge notch bending. Ring stiffness of such pipes which, are considered characteristic properties, is numerically evaluated using the extended finite element method before and after preconditioning. The results reported that both tensile and compressive strengths degraded nearly more than 20%. Besides the fracture toughness decrease, the stiffness ring strength is reduced, and the finite element results are in good agreement with the experimental findings.
Use of Digital Educational Technologies among Nursing Students and Teachers: An Exploratory Study
Authors: Loureiro Fernanda , Sousa Luís , Antunes Vanessa
Published: October 2021

The emergence of digital educational technologies (DET) raises questions regarding the personalization of both teaching and care. DET use implies profound changes with consequences in nursing care and in nursing teaching-learning process. With the purpose of contributing to the improvement of the teaching-learning process through the use of DET, an exploratory-descriptive, cross-sectional, and observational study, with a quantitative approach (descriptive and inferential statistics), was developed. Online questionnaires were applied (n = 140 students and n = 23 teachers) after ethics committee approval. Results point to low cost and access without time/space limits as the main benefits, and decreased interaction, less physical contact, and technical difficulties as constraints. Globally, there was no difference between students and teachers in the use of DET. Still, men report more constraints than women. In this sample, the use of DET is still at an early stage. Both students and teachers are still unfamiliar with the scope and possibilities of these tools, not taking full advantage of the potential they have to offer. The impact of DET used in personalized nursing care is still yet to be understood.
The Diagnostic Value of 99mTc-HMPAO-Labelled White Blood Cell Scintigraphy and 18F-FDG PET/CT in Cardiac Device-Related Infective Endocarditis—A Systematic Review
Authors: Holcman Katarzyna , Rubiś Paweł , Stępień Agnieszka , Graczyk Katarzyna , Podolec Piotr , Kostkiewicz Magdalena
Published: October 2021

(1) Background: Treatment of cardiac arrhythmias and conduction disorders with the implantation of a cardiac implantable electronic device (CIED) may lead to complications. Cardiac device-related infective endocarditis (CDRIE) stands out as being one of the most challenging in terms of its diagnosis and management. Developing molecular imaging modalities may provide additional insights into CDRIE diagnosis. (2) Methods: We performed a systematic literature review to critically appraise the evidence for the diagnostic performance of the following hybrid techniques: single photon emission tomography with technetium99m-hexamethylpropyleneamine oxime–labeled autologous leukocytes (99mTc-HMPAO-SPECT/CT) and positron emission tomography with fluorodeoxyglucose (18F-FDG PET/CT). An analysis was performed in accordance with PRISMA and GRADE criteria and included articles from PubMed, Embase and Cochrane databases. (3) Results: Initially, there were 2131 records identified which had been published between 1971–2021. Finally, 18 studies were included presenting original data on the diagnostic value of 99mTc-HMPAO-SPECT/CT or 18F-FDG PET/CT in CDRIE. Analysis showed that these molecular imaging modalities provide high diagnostic accuracy and their inclusion in diagnostic criteria improves CDRIE work-up. (4) Conclusions: 99mTc-HMPAO-SPECT/CT and 18F-FDG PET/CT provide high diagnostic value in the identification of patients at risk of CDRIE and should be considered for inclusion in the CDRIE diagnostic process.
Is Prevention of Postoperative Vomiting Surgery Dependent? A Retrospective Cohort Study of Total Knee Arthroplasty
Authors: Poon Yan-Yuen , Hung Kuo-Chuan , Chou Wen-Yi , Wang Chih-Hsien , Hung Chao-Ting , Chin Jo-Chi , Wu Shao-Chun
Published: October 2021

The study of postoperative nausea and vomiting (PONV) has been ongoing since the early days of general anesthesia. The search for risk factors of PONV and the development of new agents to treat PONV are the two main strategies to combat the adverse side effects of general anesthesia. Female sex, non-smoking status, a history of PONV/motion sickness, and postoperative opioid use are the four independent risk factors for PONV derived after a series of prospective studies, evidence-based systematic reviews, and meta-analyses. The two frequently asked questions that arise ask whether risk factors apply to different clinical settings and whether prevention measures of PONV can be surgery dependent. We conducted a comprehensive review of 665 patients who underwent primary total knee arthroplasty (TKA) between January and December 2019. As nausea is subjective and its measurement is not standardized, postoperative vomiting (POV) was used as a study endpoint. The exclusion criteria were desflurane anesthesia, spinal anesthesia, anesthesia without bispectral index monitoring, and day surgery. Three well-recognized risk factors, consisting of body weight, sevoflurane consumption, and postoperative opioid consumption, were not considered as independent risk factors of POV, while female sex, preoperative adductor canal block (ACB), and dexamethasone were identified as being so in this study. The risk of POV in the female sex was 2.49 times that in the male sex; however, when dexamethasone was used, this risk was reduced by >40% compared with no antiemetic use, and by >50% when patients received preoperative ACB compared with those without the block. The clinical characteristics of our TKA patients—female dominance, old age, and their fairly constant body weights that were distinct from those of other surgical patients—suggested that age may play a crucial role in determining the relative contributions of the different risk factors of POV. We concluded that risk factors of POV are dependent on clinical settings. Based on these results, it is reasonable to speculate that a surgery-dependent plan for the prevention of POV is feasible for patients in similar clinical settings.
An Effectiveness Study on the Use of Different Types of LID for Water Cycle Recovery in a Small Catchment
Authors: Kim Heenyun , Kim Gunwoo
Published: October 2021

Low-Impact Development (LID) is alleviating the water cycle problems that arise from an increasing impervious surface area caused by urbanization. However, there is insufficient research on the application and analyses of LID techniques that are used for studying the management goals for water cycle restoration. The present study applied various LID techniques, utilizing the stormwater management model (SWMM) in the Naju-Noan Waterfront Zone Construction Project and studying its effects, aiming to restore the runoff that had increased due to urbanization to its pre-development state. The five LID techniques used in the analysis were permeable pavements, bioswales, rainwater gardens, green roofs, and planter boxes, which took up 36.2% of the total area. Our analysis showed that development increased the runoff rate from 39.4% to 62.4%, and LID reduced it to 34.7%. Furthermore, development increased the peak flow from 0.77 m³/s to 1.08 m³/s, and the application of LID reduced it to 0.78 m³/s. An effective reduction in the runoff and peak flow was shown in every recurrence period that was tested, and the bioretention cell type of LID showed the best effectiveness per unit area compared with permeable pavements and green roofs.
Elucidating Traditional Rice Varieties for Consilient Biotic and Abiotic Stress Management under Changing Climate with Landscape-Level Rice Biodiversity
Authors: Muralikrishnan L. , Padaria Rabindra. N. , Dass Anchal , Choudhary Anil K. , Kakade Bharat , Shokralla Shadi , El-Abedin Tarek K. Zin , Almutairi Khalid F. , Elansary Hosam O.
Published: October 2021

Rice is grown under diverse agro-climatic conditions and crop management regimes across the globe. Emerging climatic-vulnerabilities and the mismatched farm practices are becoming major challenges for poor or declining rice productivity in potential rice growing regions, especially South Asia. In the biodiversity-rich landscapes of South Asia, many traditional rice varieties (TRVs) are known to exhibit resilience to climate change and climate adaptation besides their therapeutic benefits. Hence, a random sample survey of farmers (n = 320), alongwith secondary data collection from non-governmental organizations/farmers’ organizations/farmers, led to documentation of the information on TRVs’ biodiversity in South Asia. The current study (2015–2019) explored and documented ~164 TRVs which may enhance the resilience to climatic-risks with improved yields besides their unique therapeutic benefits. A large number of TRVs have still not been registered by scientific organizations due to poor awareness by the farmers and community organizations. Hence, it is urgently needed to document, evaluate and harness the desired traits of these TRVs for ecological, economic, nutritional and health benefits. This study suggests taking greater cognizance of TRVs for their conservation, need-based crop improvement, and cultivation in the niche-areas owing to their importance in climate-resilient agriculture for overall sustainable rice farming in South Asia so as to achieve the UN’s Sustainable Development Goals.
Soil Restoration through the Application of Organic Mulch Following Skidding Operations Causing Vehicle Induced Compaction in the Hyrcanian Forests, Northern Iran
Authors: Khoramizadeh Azadeh , Jourgholami Meghdad , Jafari Mohammad , Venanzi Rachele , Tavankar Farzam , Picchio Rodolfo
Published: October 2021

In this study an attempt was made to assess how different mulches affect the soil environment. In particular, different organic mulches such as leaf litter, straw and sawdust were tested in order to assess their capacities to amend the soil conditions. These analyses were carried out in the Hyrcanian mixed broadleaved forest. Organic mulches can compensate the litter layer loss on compaction-induced soil and accelerate the restoration process of soil properties, which takes from a few years to several decades without mulching. However, comprehensive knowledge on the effects of organic mulch on soil quality in terms of compaction-induced soil in the scientific literature is still scarce and inadequate. The main aim of the study was to examine the effects of three organic mulches (leaf litter, straw and sawdust) on the restoration of forestry vehicle-induced soil properties in the skid trail over a 2-year period. The results showed as the values of soil physical and chemical properties in litter, straw and sawdust treatments were significantly restored as compared with the values in the untreated soil. In general, leaf litter supplies nutrients at higher rates than the straw and sawdust mulches. However, according to the current results, a 2-year period is not enough to return the soil physical and chemical properties to pre-traffic levels. Furthermore, the present study shows that organic mulch spread on the surface of mineral soil in the skid trails after machine traffic acts as a fertilizer to accelerate the decomposition of organic matter.
Mechano-Immunomodulation in Space: Mechanisms Involving Microgravity-Induced Changes in T Cells
Authors: Dhar Sarit , Kaeley Dilpreet Kaur , Kanan Mohamad Jalal , Yildirim-Ayan Eda
Published: October 2021

Of the most prevalent issues surrounding long-term spaceflight, the sustainability of human life and the maintenance of homeostasis in an extreme environment are of utmost concern. It has been observed that the human immune system is dysregulated in space as a result of gravitational unloading at the cellular level, leading to potential complications in astronaut health. A plethora of studies demonstrate intracellular changes that occur due to microgravity; however, these ultimately fall short of identifying the underlying mechanisms and dysfunctions that cause such changes. This comprehensive review covers the changes in human adaptive immunity due to microgravity. Specifically, there is a focus on uncovering the gravisensitive steps in T cell signaling pathways. Changes in gravitational force may lead to interrupted immune signaling cascades at specific junctions, particularly membrane and surface receptor-proximal molecules. Holistically studying the interplay of signaling with morphological changes in cytoskeleton and other cell components may yield answers to what in the T cell specifically experiences the consequences of microgravity. Fully understanding the nature of this problem is essential in order to develop proper countermeasures before long-term space flight is conducted.
High-Throughput Sequencing of Circulating MicroRNAs in Plasma and Serum during Pregnancy Progression
Authors: Vashukova Elena S. , Kozyulina Polina Y. , Illarionov Roman A. , Yurkina Natalya O. , Pachuliia Olga V. , Butenko Mariya G. , Postnikova Tatyana B. , Ivanova Lada A. , Eremeeva Dina R. , Zainulina Marina S. , Bespalova Olesya N. , Glotov Andrey S.
Published: October 2021

Although circulating microRNAs (miRNAs) in maternal blood may play an important role in regulation of pregnancy progression and serve as non-invasive biomarkers for different gestation complications, little is known about their profile in blood during normally developing pregnancy. In this study we evaluated the miRNA profiles in paired plasma and serum samples from pregnant women without health or gestational abnormalities at three time points using high-throughput sequencing technology. Sequencing revealed that the percentage of miRNA reads in plasma and serum decreased by a third compared to first and second trimesters. We found two miRNAs in plasma (hsa-miR-7853-5p and hsa-miR-200c-3p) and 10 miRNAs in serum (hsa-miR-203a-5p, hsa-miR-495-3p, hsa-miR-4435, hsa-miR-340-5p, hsa-miR-4417, hsa-miR-1266-5p, hsa-miR-4494, hsa-miR-134-3p, hsa-miR-5008-5p, and hsa-miR-6756-5p), that exhibit level changes during pregnancy (p-value adjusted < 0.05). In addition, we observed differences for 36 miRNAs between plasma and serum (p-value adjusted < 0.05), which should be taken into consideration when comparing the results between studies performed using different biosample types. The results were verified by analysis of three miRNAs using qRT-PCR (p < 0.05). The present study confirms that the circulating miRNA profile in blood changes during gestation. Our results set the basis for further investigation of molecular mechanisms, involved in regulation of pregnancy, and the search for biomarkers of gestation abnormalities.
Current Therapeutic Approach to Acute Myocardial Infarction in Patients with Congenital Hemophilia
Authors: Badescu Minerva Codruta , Ciocoiu Manuela , Rezus Elena , Badulescu Oana Viola , Tanase Daniela Maria , Ouatu Anca , Dima Nicoleta , Ganceanu-Rusu Ana Roxana , Popescu Diana , Seritean Isac Petronela Nicoleta , Genes Tudor-Marcel , Rezus Ciprian
Published: October 2021

Advances in the treatment of hemophilia have made the life expectancy of hemophiliacs similar to that of the general population. Physicians have begun to face age-related diseases not previously encountered in individuals with hemophilia. Treatment of acute myocardial infarction (AMI) is particularly challenging because the therapeutic strategies influence both the patient’s thrombotic and hemorrhagic risk. As progress has been made in the treatment of AMI over the last decade, we performed an in-depth analysis of the available literature, highlighting the latest advances in the therapy of AMI in hemophiliacs. It is generally accepted that after the optimal substitution therapy has been provided, patients with hemophilia should be treated in the same way as those in the general population. New-generation stents that allow short dual antiplatelet therapy and potent P2Y12 receptor inhibitors have begun to be successfully used. At a time when specific recommendations and relevant data are scarce, our study provides up-to-date information to physicians involved in the treatment of AMI in hemophiliacs.
Effect of Polyphenylsulfone and Polysulfone Incompatibility on the Structure and Performance of Blend Membranes for Ultrafiltration
Authors: Plisko Tatiana , Karslyan Yana , Bildyukevich Alexandr
Published: October 2021

This study deals with the modification of polyphenylsulfone ultrafiltration membranes by introduction of an incompatible polymer polysulfone to the polyphenylsulfone casting solution to improve the permeability. The correlation between properties of the blend polyphenylsulfone/polysulfone solutions and porous anisotropic membranes for ultrafiltration prepared from these solutions was revealed. The blend polyphenylsulfone/polysulfone solutions were investigated using a turbidity spectrum method, optical microscopy and measurements of dynamic viscosity and turbidity. The structure of the prepared blend flat sheet membranes was studied using scanning electron microscopy. Membrane separation performance was investigated in the process of ultrafiltration of human serum albumin buffered solutions. It was found that with the introduction of polysulfone to the polyphenylsulfone casting solution in N-methyl-2-pyrrolidone the size of supramolecular particles significantly increases with the maximum at (40–60):(60:40) polyphenylsulfone:polysulfone blend ratio from 76 nm to 196–354 nm. It was shown that polyphenylsulfone/polysulfone blend solutions, unlike the solutions of pristine polymers, are two-phase systems (emulsions) with the maximum droplet size and highest degree of polydispersity at polyphenylsulfone/polysulfone blend ratios (30–60):(70–40). Pure water flux of the blend membranes passes through a maximum in the region of the most heterogeneous structure of the casting solution, which is associated with the imposition of a polymer-polymer phase separation on the non-solvent induced phase separation upon membrane preparation. The application of polyphenylsulfone/polysulfone blends as membrane-forming polymers and polyethylene glycol (Mn = 400 g·mol−1) as a pore-forming agent to the casting solutions yields the formation of ultrafiltration membranes with high membrane pure water flux (270 L·m−2·h−1 at 0.1MPa) and human serum albumin rejection of 85%.
Development and Characterization of Lightweight Geopolymer Composite Reinforced with Hybrid Carbon and Steel Fibers
Authors: Baziak Agnieszka , Pławecka Kinga , Hager Izabela , Castel Arnaud , Korniejenko Kinga
Published: October 2021

The aim of this paper is to analyze the influence of hybrid fiber reinforcement on the properties of a lightweight fly ash-based geopolymer. The matrix includes the ratio of fly ash and microspheres at 1:1. Carbon and steel fibers have been chosen due to their high mechanical properties as reinforcement. Short steel fibers (SFs) and/or carbon fibers (CFs) were used as reinforcement in the following proportions: 2.0% wt. CFs, 1.5% wt. CFs and 0.5% wt. SFs, 1.0% wt. CFs and 1.0% wt. SFs, 0.5% wt. CFs and 1.5% wt. SFs and 2.0% wt. SFs. Hybrid reinforcement of geopolymer composites was used to obtain optimal strength properties, i.e., compressive strength due to steel fiber and bending strength due to carbon fibers. Additionally, reference samples consisting of the geopolymer matrix material itself. After the production of geopolymer composites, their density was examined, and the structure (using scanning electron microscopy) and mechanical properties (i.e., bending and compressive strength) in relation to the type and amount of reinforcement. In addition, to determine the thermal insulation properties of the geopolymer matrix, its thermal conductivity coefficient was determined. The results show that the addition of fiber improved compressive and bending strength. The best compressive strength is obtained for a steel fiber-reinforced composite (2.0% wt.). The best bending strength is obtained for the hybrid reinforced composite: 1.5% wt. CFs and 0.5% wt. SFs. The geopolymer composite is characterized by low thermal conductivity (0.18–0.22 W/m ∙ K) at low density (0.89–0.93 g/cm3).
Ultrasonic Vibration-Assisted Ball Burnishing Tool for a Lathe Characterized by Acoustic Emission and Vibratory Measurements
Authors: Fernández-Osete Ismael , Estevez-Urra Aida , Velázquez-Corral Eric , Valentin David , Llumà Jordi , Jerez-Mesa Ramón , Travieso-Rodriguez J. Antonio
Published: October 2021

This paper focuses on a resonant system used to induce a low-amplitude movement and ultrasonic frequency to complement a ball burnishing process on a lathe. The system was characterized through the combination of different techniques. A full vibratory characterization of this process was undertaken with the purpose of demonstrating that the mechanical system—composed of the tool and the machine—does not present resonance phenomena during the execution of the operation that could lead to eventual failure. This dynamic analysis validates the adequateness of the tool when attached to an NC lathe, which is important to guarantee its future implementation in actual manufacturing contexts. A further aim was to confirm that the system succeeds in transmitting an oscillating signal throughout the material lattice. To this end, different static and dynamic techniques that measure different vibration ranges—including impact tests, acoustic emission measurement, and vibration measurement—were combined. An operational deflection shape model was also constructed. Results demonstrate that the only high frequency appearing in the process originated in the tool. The process was not affected by the presence of vibration assistance, nor by the burnishing preload or feed levels. Furthermore, the frequency of the assisting ultrasonic vibration was characterized and no signal due to possible damage in the material of the specimens was detected. These results demonstrate the suitability of the new tool in the vibration-assisted ball burnishing process.
In-Situ Synthesis of Methyl Cellulose Film Decorated with Silver Nanoparticles as a Flexible Surface-Enhanced Raman Substrate for the Rapid Detection of Pesticide Residues in Fruits and Vegetables
Authors: Zhang Qijia , Xu Guangda , Guo Na , Wang Tongtong , Song Peng , Xia Lixin
Published: October 2021

The purpose of this study was to develop a flexible substrate methylcellulose-decorated silver nanoparticles (MC/Ag NPs) film and explore its application in fruits and vegetables by surface enhanced Raman spectroscopy (SERS) technology for rapid detection of pesticides. The performance of the MC/Ag NPs film substrate was characterized by Nile blue A (NBA), and the detection limit was as low as 10−8 M. The substrate also exhibited satisfactory Raman signal strength after two months of storage. The impressive sensitivity and stability were due to the excellent homogeneity of the silver nanoparticles that were grown in situ in the methylcellulose matrix, which generated “hot spots” between the silver nanoparticles without a large amount of aggregation, and resulted in the ultra-high sensitivity and excellent stability of the MC/Ag NPs film substrate. The MC/Ag NPs film substrate was used to detect thiram pesticides on tomato and cucumber peels, and the minimum detectable level of thiram was 2.4 ng/cm2, which was much lower than the maximum residue level. These results indicate that the MC/Ag NPs film is sensitive to rapid detection of multiple pesticides in food.
Physicochemical and Biological Properties of Graphene-Oxide-Coated Metallic Materials
Authors: Poniatowska Aleksandra , Trzaskowska Paulina Anna , Trzaskowski Maciej , Ciach Tomasz
Published: October 2021

In this article, we present graphene oxide (produced by a modified Hummers’ method) coatings obtained using two different methods: electrophoretic deposition on 316L stainless steel and chemical modification of the surface of gold applied to the steel. The coating properties were characterized by microscopic and spectrometric techniques. The contact angle was also determined, ranging from 50° to 70°. Our results indicated that GO coatings on steel and gold were not toxic towards L929 cells in a direct cell adhesion test—on all tested materials, it was possible to observe the growth of L929 cells during 48 h of culture. The lack of toxic effect on cells was also confirmed in two viability tests, XTT and MTT. For most of the tested materials, the cell viability was above 70%. They showed that the stability of the coating is the crucial factor for such GO coatings, and prove that GO in the form of coating is non-toxic; however, it can show toxicity if detached from the surface. The obtained materials also did not show any hemolytic properties, as the percentage of hemolysis was on the level of the negative control, which is very promising in the light of future potential applications.
Powder Metallurgical Processing and Characterization of Molybdenum Addition to Tungsten Heavy Alloys by Spark Plasma Sintering
Authors: Annamalai A. Raja , Muthuchamy A. , Srikanth Muthe , Natarajan Senthilnathan , Acharya Shashank , Khisti Anup , Jen Chun-Ping
Published: October 2021

The effect of adding molybdenum to the heavy tungsten alloy of W-Ni-Fe on its material characteristics was examined in the current study. The elemental powders of tungsten, iron, nickel, and molybdenum, with a composition analogous to W-3Fe-7Ni-xMo (x = 0, 22.5, 45, 67.5 wt.%), were fabricated using the spark plasma sintering (SPS) technique at a sintering temperature of 1400 °C and under pressure of 50 MPa. The sintered samples were subjected to microstructural characterization and tested for mechanical strength. The smallest grain size of 9.99 microns was observed for the 45W-45Mo alloy. This alloy also gave the highest tensile and yield strengths of 1140 MPa and 763 MPa, respectively. The hardness increased with the increased addition of molybdenum. The high level of hardness was observed for 67.5Mo with a 10.8% increase in the base alloy’s hardness. The investigation resulted in the alloy of 45W-7Ni-3Fe-45Mo, observed to provide optimum mechanical properties among all the analyzed samples.
Adsorption of Organosilanes on the Surface of Aluminium and the Formation of Organosilane Films to Protect It from Corrosion
Authors: Gladkikh Natalia , Petrunin Maxim , Maksaeva Ludmila , Yurasova Tatyana
Published: October 2021

Adsorption of diaminesilane (DAS), vinyltrimethoxysilane (VS) on the surface of thermally precipitated aluminium was examined. The use of different adsorption isotherms made it possible to calculate the adsorption heats for DAS and VS. It was determined that chemisorption of these organosilanes occurred on the surface of aluminium. Exposure of aluminium for 60 min to aqueous solutions of organosilanes led to the formation of organosilane films on the surface of the metal. The use of infrared spectroscopy and scanning electron microscopy in the work made it possible to assess the interactions of organosilanes with the metal surface, as well as to determine the structural features of the films and their thickness. Electrochemical and corrosion research methods made it possible to study the protective properties of organosilane films on aluminium.
The Formation of 14H-LPSO in Mg–9Gd–2Y–2Zn–0.5Zr Alloy during Heat Treatment
Authors: Liu Yunfang , Yang Yaqin , Yi Ming , Yu Jianmin , Li Baocheng , Zhang Zhimin
Published: October 2021

There is a new long-period stacking ordered structure in Mg–RE–Zn magnesium alloys, namely the LPSO phase, which can effectively improve the yield strength, elongation, and corrosion resistance of Mg alloys. According to different types of Mg–RE–Zn alloy systems, two transformation modes are involved in the heat treatment transformation process. The first is the alloy without LPSO phase in the as-cast alloy, and the MgxRE phase changes to 14H-LPSO phase. The second is the alloy containing LPSO phase in the as-cast state, and the 14H-LPSO phase is obtained by the transformations of 6H, 18R, and 24R. The effects of different solution parameters on the second phase of Mg–9Gd–2Y–2Zn–0.5Zr alloy were studied by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The precipitation mechanism of 14H-LPSO phase during solution treatment was further clarified. At a solution time of 13 h, the grain size increased rapidly initially and then decreased slightly with increasing solution temperature. The analysis of the volume fraction of the second phase and lattice constant showed that Gd and Y elements in the alloy precipitated from the matrix and formed 14H-LPSO phase after solution treatment at 490 °C for 13 h. At this time, the hardness of the alloy reached the maximum of 74.6 HV. After solution treatment at 500 °C for 13 h, the solid solution degree of the alloy increases, and the grain size and hardness of the alloy remain basically unchanged.
Optimization of Hyaluronate-Based Liposomes to Augment the Oral Delivery and the Bioavailability of Berberine
Authors: Kutbi Hussam I. , Asfour Hani Z. , Kammoun Ahmed K. , Sirwi Alaa , Cavalu Simona , Gad Heba A.
Published: October 2021

Various perspectives had been utilized to enhance the poor intestinal permeability and bioavailability of drugs with low water solubility. Berberine (Brb) is a unique molecule that possesses multiple therapeutic activities such as antimicrobial, anti-inflammatory, antioxidant and anti-hyperglycemic effects. To improve Brb permeability and bioavailability, this study presents a newly developed formulation, namely Brb hyaluronate-based liposomes, prepared by using film hydration method and characterized by dynamic light scattering measurements, entrapment efficiency percentage (EE%), transmission electron microscope (TEM), in vitro drug release and physical stability. The bioavailability of the selected formulations was assessed in vivo after oral administration to rats. The results revealed an enhanced effect of hyaluronic acid on the entrapment efficiency, reaching 78.1 ± 0.1% with mean size 520.7 ± 19.9 nm. Sustained release of Brb was recorded up to 24 h in comparison to Brb solution. Physical stability was maintained for three months at refrigeration temperature. Results of pharmacokinetics studies indicated the potential of the liposomal formulation to increase the oral bioavailability of Brb and to accelerate its entry into the bloodstream. The obtained results are accredited to the lipophilic nature of the prepared system, resembling the structural features of bio-membrane, in addition to their small size that enhances intestinal penetration.