bluuebunny/crossref_metadata_2025 huggingface dataset:
Modell zum Erlernen der Venenpunktionstechnik beim Hund
Authors: GALLE URSULA , BUBNA‐LITTITZ H.
Published: August 2010

Zum Üben der intravenösen Injektion bzw. der Blutabnahme beim Hund wird ein Übungsmodell vorgestellt, das sich bereits gut bewährt hat. Das formalinfixierte Präparat einer Hundeextremität, an dem die Vena cephalica durch einen Silikonschlauch ersetzt wird, ist einfach und billig herzustellen und hat den Vorteil, die Gegebenheiten am lebenden Versuchstier sehr gut zu simulieren. Durch die am Modell erworbene korrekte Injektionstechnik und die verminderte Zahl der Venenpunktionsversuche am lebenden Tier kann die Belastung der Versuchshunde auf ein Minimum reduziert werden.

Model for teaching venous puncture technique in the dog Possibility of reducing stress of live experimental animals in student teaching

For obtaining practice in intravenous injection or bleeding in the dog a practice model is described which has given good results. A formalin‐fixed preparation of a canine extremity in which the cephalic vein is replaced by a silicon tube is simple and cheap to set up and has the advantage of simulating very well the conditions in the living animal. Using this model, the correct technique for injection and the reduced number of venous puncture attempts in the live animal can reduce to a minimum the stress imposed on experimental dogs.

Modèle d'enseignement de la technique de ponction veineuse chez le chien. Une possibilité de diminuer la contrainte sur des animaux d'expérience dans l'enseignement

Un modèle d'exercice pour l'exercice d'injection intraveineuse et du prélèvement de sang chez le chien est présenté. Une préparation d'un membre de chien fixé à la formaline et dont Vena cephalica a été remplacée par un tuyau en silicon est facile à exécuter et possède l'avantage de simuler très bien l'expérience sur un animal vivant. La technique d'injection ainsi acquise sur le modèle et la diminution du nombre d'essais de ponctions veineuses sur l'animal vivant, permettent de réduire à un minimum la contrainte exercée sur des chiens d'expérience.

Modelo de enseñanza de la técnica de punción intravenosa en el perro Una posibilidad para reducir la violencia ejercida frente a animales vivos de ensayo en los Centros de enseñanza

Para ejercitar la inyección intravenosa o la toma de una muestra de sangre en el perro se présenta un modelo de ejercicio, el cual ha dado ya frutos satisfactorios. La preparación de una extremidad de perro, fijada en formalina, en la cual se ha reemplazado la vena cefálica por un tubo de silicona, es de ejecucion fácil y barata, teniendo la ventaja de simular muy bien las circunstancias que se hallan en un animal vivo de experiencia. Mediante la técnica de inyección, adquirida de forma correcta en el modelo, y el número reducido de intentonas en el animal vivo, se pueden reducir a un mínimo las intemperancias cometidas con los perros de ensayo.
Pepsinogenaktivität von Serum und Harn sowie Pepsinaktivität des Labmagensaftes labmagengesunder und nicht‐parasitär labmagenkranker Rinder*
Authors: Vörös K. , Meyer C. , Stöber M.
Published: August 2010

Die an klinisch gesunden Kühen (40) und Färsen (10) sowie erwachsenen weiblichen Rindern mit links‐ bzw. rechtsseitiger Labmagenverlagerung (30 bzw. 28) und Patienten mit Abomasitis oder Ulcus abomasi (6) vorgenommenen Untersuchungen des Serum‐ und Uropepsinogengehaltes sowie des Labmagenpepsines zeigen, daß auch bei nicht‐parasitär bedingter Abomasitis des Rindes mit einer Steigerung der Serumpepsinogenaktivität zu rechnen ist. Der Bestimmung des Uropepsinogenspiegels war im hier geprüften Zusammenhang keine diagnostische Aussagekraft nachzuweisen.

Pepsinogen activity of serum and urine and pepsin activity of abomasal juice of cattle with healthy and non‐parasitized abomasum

Observations on clinically healthy cows (40) and heifers (10), as well as on female cattle with left‐ or right‐sided abomasal displacement (30 and 28 cattle, respectively), together with patients with abomasitis or abomasal ulceration (6) included measurement of the uropepsinogen content and abomasal pepsin level; the results revealed that also in bovine abomasitis not associated with parasites there occurs a rise in serum pepsinogen activity. Determination of uropepsinogen levels had no diagnostic significance.

Activité du pepsinogène du sérum et de l'urine ainsi que l'activité de la pepsine du suc de la caillette chez des bovins avec une caillette saine ou avec une caillette malade mais pas d'origine parasitaire

Les recherches du taux de pepsinogène du sérum et de l'urine ainsi que de la pepsine de la caillette faites sur des vaches (40) et des génisses (10) en bonne santé ainsi que sur des vaches avec torsion gauche ou droite de la caillette (30 et 28) et sur des bêtes atteintes d'abomasite et d'Ulcus abomasi (6) ont montré qu'il fallait compter avec une augmentation de l'activité du pepsinogène sérique également lors d'abomasite non parasitaire chez le bovin. La détermination du taux de pepsinogène de l'urine n'a pas eu de signification dans le diagnostic au cours de ces recherches.

Las actividades pepsinógenas del suero sanguíneo y de la orina así como la actividad de la pepsina del jugo del cuajar de vacunos con abomaso sano o enfermo no parasitado

Muestran los estudios, efectuados en vacas (40) y novillas (10) sanas clínicamente así como en vacas adultas con dislocación del cuajar hacia la izquierda resp. hacia la derecha (30 resp. 28) y en pacientes con abomasitis y úlcera en el abomaso (6) sobre los contenidos de pepsinógeno en el suero sanguíneo y en la orina así como sobre la pepsina en el cuajar, que también en una abomasitis del ganado vacuno que no tenga origen parasitario hay que contar con un acrecentamiento en la actividad seropepsinógena. A la valoración del nivel de uropepsinógeno no se le podía reconocer en el nexo estudiado aquí ninguna fuerza de aseveración diagnóstica.
Studies on the Immunogenic Properties of Tissue‐Culture Sheep Pox Virus
Authors: Ramyar H.
Published: August 2010

A combination of lamb kidney and lamb testis cells was successfully used for the large‐scale production of sheep pox virus.

Tissue culture virus from the 8th and 12th passages showed higher titers than the initial seed material.

Vaccine of tissue culture origin established a solid immunity in inoculated animals. From the point of safety, however, the ratio of virus to aluminium gel should not exceed 1:500.

Untersuchungen über die immungenen Eigenschaften von Gewebekultur‐Schafpockenvirus

Für die Massenproduktion von Schafpockenvirus wurde mit Erfolg eine Kombination von Lammnieren‐ und Lammhodenzellen verwendet. Virus der 8. und 12. Passage aus Gewebekultur zeigte höhere Titer als das für die Erstbeimpfung benutzte Material. Vakzine aus Gewebekulturen führte zu einer guten Immunität der geimpften Tiere. Aus Sicherheitsgründen sollte jedoch das Verhältnis von Virusmenge zu Aluminiumhydroxyd‐Gel 1:500 nicht überschreiten.

Recherches sur les propriétés immunogènes du virus de la variole ovine obtenu par culture de tissu

Pour la production en grande quantité du virus de la variole ovine, on utilisa avec succès une combinaison de cellules de rein d'agneau et de testicule d'agneau. Le virus des 8e et 12e passages de la culture de tissu présentait un titre plus élevé que le matériel utilisé pour l'ensemencement initial. Des vaccins préparés à partir de la culture de tissu donnèrent une bonne immunité aux animaux vaccinés. Pour des raisons de sécurité il ne faudrait pas dépasser le rapport 1:500 entre la quantité de virus et le gel d'hydroxyde d'aluminium.

Estudios sobre las propiedades inmunógenas del virus variólico ovino en cultivo hístico

Para la producción masiva de virus variólico se utilizó con éxito una combinación de células renales y testiculares de cordero. Virus del 8° y 12° pase por cultivo hístico presentaban títulos superiores que el material empleado para a inoculación primaria. Vacunas obtenidas con cultivo hístico lograron una buena inmunidad en los animales inoculados. Sin embargo, por razones de seguridad, no debería rebasarse la relación de cantidad de virus a gel de hidróxido de aluminio de 1:500.
Steigerung der Resistenz gegen parasitäre Infektionen durch Ernährungsumstellung und Nebennierenektomie*
Authors: Schole J. , Sallmann H.‐P. , Harisch G. , Dey‐Hazra A. , Enigk K.
Published: August 2010

In drei Rattenversuchen wurde überprüft, ob die Resistenz der Tiere gegen parasitäre Infektionen (Fasciola hepatica und Nippostrongylus brasiliensis) durch die Diätgestaltung bzw. durch Nebennierenektomie geändert werden kann. Es ließ sich zeigen, daß sowohl durch Austausch von Kohlenhydrat‐ gegen Fettkalorien (15 bzw. 30% Rindertalg/Schweineschmalz 1:1, 10 bzw. 20% Schweineschmalz) als auch durch Entfernung der Nebennieren vier Tage vor der Infektion die Parasitenentwicklung stark gehemmt wird. Für diese Effekte werden endokrine Faktoren verantwortlich gemacht.

Increasing resistance against parasitic infections by changing the diet or by adrenalectomy

Three experiments were carried out in rats to see whether their resistance to parasitic infections (Fasciola hepatica and Nippostrongylus brasiliensis) could be changed by altering the diet or by adrenalectomy. The results showed that changing the ratio of carbohydrate calories to fat calories (by including 15 or 30% ox suet/pig fat 1: 1, or 10 or 20% pig fat) or removal of the adrenals four days before infection produced marked inhibition of development of the parasite. Endocrine factors are considered to be responsible.

Augmantation de la résistance aux infections parasitaires par transformation du régime alimentaire ou surrénalectomie

On examine dans trois expériences sur des rats, si la résistance des animaux contre les infections parasitaires (Fasciola hepatica et Nippostrongylus brasiliensis) peut être accrue par une transformation du régime alimentaire ou une surrénalectomie. On a pu démontrer que l'échange des hydrates de carbone contre des calories lipidiques (15 resp. 30% suif bovin/saindoux 1:1; 10 resp. 20% saindoux), ainsi que l'ablation des surrénales quatre jours avant l'infection permettaient d'inhiber fortement le développement des parasites. On attribue ces effets à des facteurs endocriniens.

Aumento de la resistencia frente a infestaciones parasitarias mediante modificaciones en la alimentación y adrenectomía

En tres ensayos con ratas se revisó si se puede alterar la resistencia de los animales frente a infestaciones parasitarias (Fasciola hepatica y Nippostrongylus brasiliensis) mediante la composición de la dieta o por medio de adrenectomía. Se pudo demostrar que tanto el intercambio de las calorías hidrocarbonadas por grasas (15 ó 30% gordura de vacuno/manteca de cerdo 1: 1, 10 ó 20% manteca de cerdo) como también mediante la ablación de los adrenes cuatro días antes de la infestación se inhibe en grado sumo el desarrollo de los parásitos. De estos efectos se hacen responsables factores endocrinos.
Cell‐Mediated Immunity to Parainfluenza‐3 (PIV‐3) in Cattle Evaluation of in vivo and in vitro tests
Authors: Moreno‐López J.
Published: August 2010

Three different methods were used to measure the cell‐mediated immune response of cattle to PIV‐3, namely leucocyte migration inhibition in agar gel, lymphocyte stimulation, and skin hypersensitivity. None of the values obtained seem to correlate quantitatively with the hemagglutination‐inhibition titers in serum. The leucocyte migration‐inhibition test appears to discriminate between two strains of PIV‐3, which differ with regard to the degree of neuraminidase activity.

Zellvermittelte Immunität gegenüber Parainfluenza‐3 Virus (PIV‐3) beim Rind; Ergebnisse von in vivo und in vitro Tests

Drei verschiedene Methoden wurden angewendet, um die zellvermittelte Immunreaktion bei Rindern auf PIV‐3 zu messen, nämlich die Hemmung der Leukozytenwanderung im Agar‐gel, Lymphozytenstimulierung und Hauthypersensibilität. Keines von den gefundenen Resultaten scheint quantitativ in Wechselbeziehung mit den Titern der Hämagglutinations‐Hemmung im Serum zu stehen. Die Untersuchung der Hemmung der Leukozyten‐Wanderung scheint zwischen zwei Stämmen von PIV‐3 zu unterscheiden, die in Hinsicht auf die Stärke der Neuraminidase‐Aktivität voneinander abweichen.

Immunité cellulaire contre le virus Parainfluenza‐3 (PIV‐3) chez le bovin: résultats de tests in vivo et in vitro

On a utilisé trois méthodes pour mesurer la réaction immunilogique cellulaire avec PIV‐3 chez des bovins: inhibition de la migration leucocytaire en gélose d'agar, stimulation des lymphocytes et hypersensibilité cutanée. Aucun des résultats trouvés ne peut se comparer quantitativement avec les titres sérologiques de l'inhibition de l'hémagglutination. La recherches de l'inhibition de la migration leucocytaire semble être différente entre deux souches PIV‐3 qui divergent du point de vue de l'intensité de l'activité de la neuraminidase.

Inmunidad de intercesión celular frente a virus parainfluenza‐3 (PIV‐3) en vacunos; resultados de pruebas in vivo e in vitro

Se recurrió a tres métodos diferentes para medir la reacción inmunológica de intercesión celular frente a PIV‐3 en vacunos, a saber, inhibición de la migración leucocitaria en gelosa de agar, estimulación linfocítica e hipersensibilidad cutánea. Ninguno de los resultados hallados parece estar en correlación cuantitativa con los títulos de inhibición de la hemoaglutinación en el suero sanguíneo. El estudio de la inhibición de migración leucocitaria parece diferenciar entre dos cepas de PIV‐3, las cuales difieren entre sí con respecto a la intensidad de actividad neuraminidásica.
Comparison of Nephelometry and Single Radial Immunodiffusion for the Determination of IgG and IgM Concentrations in Newborn Foals and their Dams1
Authors: Eisenhauer P. , Lambrecht G. , Petzoldt K. , Henkel E.
Published: August 2010

A total of 225 sera, 45 colostral and 45 milk samples were taken from 45 mares and their foals at parturition and in the first week post partum. Total protein values of the samples were estimated by the Biuret method and concentrations of IgG and IgM were determined by nephelometry and by single radial immunodiffusion and the results were compared. A good correlation was found between the results of the two tests but both techniques have some drawbacks. The nephelometric method is costly and requires higher quality antisera than are at present available commercially.

Vergleich von Nephelometrie und einfacher radialer Immunodiffusion zur Bestimmung der IgG‐ und IgM‐Konzentration in neugeborenen Fohlen und ihren Müttern

225 Serum‐, 45 Kolostrum‐ und 45 Milchproben von 45 Stuten und ihren Fohlen zum Zeitpunkt der Geburt und in der ersten Woche post partum gemessen. Der Gesamtproteingehalt der Proben wurde mit der Biuretmethode bestimmt und die IgG‐ und IgM‐Konzentration vergleichend mit der Nephelometrie und der einfachen radialen Immunodiffusion gemessen. Es konnte eine gute Korrelation der Ergebnisse für IgG und IgM zwischen den beiden Testsystemen gefunden werden, doch haben beide Methoden einige Unzulänglichkeiten.

Comparaison de la néphelométrie et d'une immunodiffusion radiale simple pour la détermination des concentrations en IgG et IgM chez des poulains nouveaux‐nés et leurs mères

On a mesuré 225 échantillons de sérums, de colostrum et de lait chez 45 juments et leurs poulains au moment de la naissance et dans la première semaine post partum. Le taux total des protéines a été déterminé par la méthode de Biuret et la concentration des IgG et IgM en comparaison avec la néphelométrie et une immunodiffusion radiale simple. Les deux tests ont donné une bonne corrélation pour les IgG et les IgM, mais les deux méthodes ont présenté quelques insuffisances.

Comparación de la nefelometría e inmunodifusión radial simple para determinar las concentraciones de IgG e IgM en potros recien nacidos y en sus madres

Se valoraron 225 muestras de suero sanguíneo, 45 de calostro y 45 de leche de yeguas y de sus potros en el momento del parto y en las semanas primeraspost partum.El contenido en proteínas totales de las muestras se determinó con el método del biuret, y las concentraciones de IgG e IgM comparativamente con la nefelometría y la inmunodifusión radial simple. Se pudo establecer una correlación buena de los resultados para IgG e IgM entre ambos sistemas de pruebas, pero hay que advertir que ambas técnicas tienen algunas insuficiencias.
Isolation of Mononuclear Cells from the Peripheral Blood of Camels (Camelus dromedarius)
Authors: Zweygarth E.
Published: August 2010

Mononuclear cells (MNC) from the peripheral blood of dromedary camels were isolated on a Percoll density gradient with a density of 1,081 g/ml. The procedure resulted in the preparation of cells with a yield of 57.9–99% MNC, and a purity of 98.8%. Within the MNC population an average of 5.3% of the cells were monocytes as judged by latex phagocytosis. The cell viability was 97% or more.

Isolierung mononukleärer Zellen aus dem peripheren Blut von Dromedaren (Camelus dromedarius)

Mononukleäre Zellen aus dem peripheren Blut von Dromedaren wurden über einen Percoll Dichtegradienten mit einer spezifischen Dichte von 1,081 g/ml isoliert. Die Zell‐ausbeute betrug 57,9—99% MNC mit einer durchschnittlichen Reinheit von 98,8%. Die Population der MNC enthielt durchschnittlich 5,3% Monozyten, deren Anzahl durch Latex‐Phagozytose bestimmt wurde. Die Viabilität der Zellen betrug 97% und mehr.

Isolement de cellules mononucléées à partir du sang périphérique de dromadaires (Camelus dromedarius)

Des cellules mononucléées ont été isolées du sang périphérique de dromadaires par un gradient d'épaisseur Percoll avec une densité de 1,081 g/ml. Le rendement cellulaire fut de 57,9 à 99% MNC avec une pureté moyenne de 98,8%. La population des MNC contenait en moyenne 5,3% de monocytes dont le nombre fut déterminé par phagocytose sur latex. La viabilité des cellules fut de 97% et plus.

El aislamiento de células mononucleadas de la sangre periférica de dromedarios (Camelus dromedarius)

Se aislaron células mononucleadas de la sangre periférica de dromedarios por un gradiente de espesor Percoll con una densidad específica de 1081 g/ml. El rendimiento celular fue del orden de 57,9–99% MNC con una pureza media de 98,8%. La población de MNC contenía como media 5,3% de monocitos, cuyo número fue determinado por medio de fagocitosis en lt́ex. La viabilidad de las células fue de 97% y más.
Endometrial Cancer Detected 31 Months after Childbirth – A Case Report –
Authors: Yajima Akira , Kyozuka Mitsuo , Wu Tzong Chung
Published: August 2010

A case of endometrial cancer, detected and treated 2 years and 7 months after childbirth, is reported.

The patient was born in 1948. After a spontaneous delivery in January of 1979, endometrial carcinoma was detected in August of 1981.

There was no history of pill usage or menstrual cycle irregularities. No associated illnesses, such as diabetes mellitus, obesity, or hypertension, were present.

Preoperative determination of hormone levels did not indicate any marked abnormalities.

Histologically, the lesion was a mixed adenosquamous carcinoma with no invasion of the myometrium.
Cervical Cancer in Association with Intrauterine Contraceptive Devices
Authors: Sugimori Hajime , Kidera Yoshiro , Nagata Yukihiro
Published: August 2010

Genital malignancy in association with intrauterine contraceptive devices (IUDs) has rarely been reported. In this paper 21 cases of cervical cancer in IUD users are presented. Close examination of the resected uteri revealed no direct contact of the IUD with the cancer nest and no abnormality suggesting malignancy around the device. These findings seem to support the general view that IUDs have no oncogenic potential on the uterine cervix. Nevertheless, Pap tests must be performed annually in healthy IUD wearers to facilitate early diagnosis of malignancy.
Re‐evaluation of Measurements of Maternal Serum hCG, hPL and Progesterone as Prognostic Markers of Abortion in Early Pregnancy
Authors: Sugita Nagatoshi , Yamaji Kenji , Takagi Tetsu , Chiba Yoshihide , Tanizawa Osamu
Published: August 2010

Maternal serum hCG, hPL and progesterone concentrations were measured by radioimmunoassay in patients with normal pregnancy, threatened abortion, missed abortion and retarded abortion in early pregnancy which could be diagnosed by real‐time ultrasonography.

Much lower hormone levels were observed in patients with missed and retarded abortion than in the other two groups; the critical levels for fetal viability were 104 mIU hCG/ml, 0.1 μg hPL/ml and 10 ng progesterone/ml.

In week 12 to 13 of pregnancy, the serum hPL and progesterone levels in patients with threatened abortion were significantly lower than those in normal pregnancy, but serum hCG levels in the two groups were not significantly different.

Serial sampling revealed that these hormone levels increased in several cases after fetal death.
Rokitansky ‐ Kuster ‐ Hauser Syndrome with Fibromyoma Arising in Rudimentary Buds
Authors: Purwar Manorama B. , Deshpande Asha S. , Jawade Shashikala B.
Published: August 2010

Unusual case of fibromyoma arising in bilateral rudimentary, noncanalized muscular buds in Rokitansky – Kuster – Hauser syndrome (RKH) was reported because of its rarity.
A Case of Extragonadal Endodermal Sinus Tumor — Histological, Cytological Aspects and Serial Determination of Serum AFP and IAP
Authors: Ito Kozo , Suzuki Hiroshi , Ikeda Masanori , Teshima Kensaku , Noda Kiichiro
Published: August 2010

A case of extragonadal endodermal sinus tumor (EST) is presented in this paper. The histological findings are identical to those described for EST by Teilum. It is suggested that the EST originated from the external longitudinal layer of the muscular tunic of the appendix. Imprinted smears for the EST were examined cytologically and serial determinations of alpha fetoprotein (AFP) and immunosuppressive acidic protein (IAP) were observed during the clinical course. As a result, it was shown that cytological examination is sufficient for diagnosis of this tumor and the status of other organs upon operation and that serial determination of serum AFP is significant as an indicator of the clinical course after an operation for EST. IAP is also considered to be valuable in assessing the immunological status and general condition of cancer patients.
Serum Zinc Levels in Pregnant Women, under Oral Contraceptive Therapy and in Normal (Ovulating) Women
Authors: Purwar Manorama B. , Deshpande Asha S. , Jawade Shashikala B.
Published: August 2010

Serum Zinc levels were estimated by atomic absorption spectrophotometer in 15 women with normal menstrual cycles, in 15 women taking oral ovulation inhibitors, and in 25 women with normal pregnancy in third trimester. In normally menstruating women a significant increase in serum Zinc levels were observed at the time of ovulation i.e. from 0.586 μgm/ml to 0.840 μgm/ml. In women taking combined contraceptive pills (orlest) Zinc levels were lower and midcycle peak was missing. The serum Zinc levels was decreased during the third trimester of pregnancy to 0.289 μgm/ml.
Use of Contraceptives in three South‐East Asian Countries
Authors: Lun Kwok‐Chan
Published: August 2010

Use of contraceptives by some selected characteristics was studied for married women in the reproductive age range (MWRA) in three south‐east Asian countries. The data were obtained from each country's national family planning/fertility survey conducted around the mid‐seventies. Only the gross relationship between each of the selected characteristics and contraceptive use was compared. The proportion of MWRA using contraceptives for Singapore was almost twice as high as the corresponding proportions for Peninsular Malaysia and Thailand. The pattern of contraceptive use was largely similar for Thailand and Malaysia but different from Singapore. The differences were largely attributed to the absence of a large rural population in Singapore, the apparently greater tendency of women in Singapore to use reversible contraceptive methods for birth spacing and the easier access by women to family planning information and services, particularly sterilisation, than in Peninsular Malaysia and Thailand.
Discrepancy of Endometrial Dating in Mid‐Luteal Phase of Hyperprolactinemic Women
Authors: Tamaya Teruhiko , Murakami Toshiki , Fujimoto Jiro , Abe Jun , Okada Hiroji
Published: August 2010

The relation between serum hormone levels, endometrial cellular steroid receptor levels and endometrial dating was evaluated in the mid‐luteal phase of normoprolactinemic and hyperprolactinemic women for a better understanding of luteal insufficiency and determination of the possible effect of prolactin on the endometrium. In 8 out of 17 luteal hyperprolactinemic patients (47%), the endometrium appeared out‐of‐phase although there was no difference in basal plasma steroid levels between normo‐ and hyper‐prolactinemic in‐phase endometria. Such out‐of‐phase endometria, however, showed significantly decreased estrogen receptor levels. These findings suggest that one of the causes of the endometrium phase discrepancy during the mid‐luteal phase may be the direct effect of prolactin on the endometrium or an indigenous disturbance of cellular steroid receptor synthesis.
Effects of Sex‐steroid Hormones on Growth and Hormone Receptor Levels of Human Uterine Leiomyosarcoma Cells in Vitro
Authors: Ishiwata Isamu , Ishiwata Chieko , Ishikawa Hiroshi
Published: August 2010

The effects of 17β‐estradiol and progesterone on SKN‐I cells (human uterine leiomyosarcoma cells) were studied for concentrations ranging from 1 pM to 100 μM in Vitro. 17β‐Estradiol enhanced cell proliferation at 1 μM or lower, but suppressed it at higher concentrations (>10 μM). On the other hand, progesterone did not affect the cells at lower than 0.1 μM, but suppressed their growth at higher than 1 μM. The cells showed degenerating morphological changes 24 hours after administration of higher than 10 μM of these hormones. In cells treated 4 days with 17μ‐estradiol (0.1 to 10 nM), basal progesterone receptor levels increased from 1.3 to 2.25 pM/mg DNA and these effects of 17β‐estradiol were suppressed by the antiestrogen tamoxifen at 1 μM.
Binding Sites for Prostaglandin F2α in Human Corpora Lutea
Authors: Tanaka Shoichi , Shimoya Yasuharu , Hamamatsu Miho , Hashimoto Masayoshi
Published: August 2010

The specific binding of 3H‐prostaglandin(PG)F2α to rough cell membrane fractions of human corpora lutea during the menstrual cycle was examined in detail.

The specific binding of 3H‐PGF2α was dependent on both duration and temperature of incubation. At 37°C total binding was maximal after 60 min and then reached equilibrium.

The presence of unlabeled PGE1, PGE2 and PGF1α had no effect on 3H‐PGF2α binding in the same rough cell membrane fractions and almost complete inhibition was seen at 7 times 10‐7M unlabeled PGF2α.

The specific 3H‐PGF2α binding to all corpora lutea was biphasic. The number of binding sites and association constants were nearly stationary throughout the luteal phase.

The concentrations of binding sites were 2 ˜ 47 and 80 ˜ 280 f moles/mg protein and the association constants were 11.0 ˜ 20.0 and 0.56 ˜ 1.20 times 1011 M‐1
Intravenous Gammaglobulin Therapy for Severe Infections Caused after Treatment for Malignant Tumors of the Female Genital Tract
Authors: Ito Haruhide , Tanaka Tadao , Kurashima Tomiyo , Ohoba Akio , Watanabe Hiroshi
Published: August 2010

A pepsin‐treated gammaglobulin preparation was intravenously administered to nineteen patients who developed severe infections after treatment for malignant tumors of the female genital tract. Fourteen of the nineteen patients (73.7%) showed a good or fair response to the gammaglobulin preparation. This preparation was especially effective against urinary tract and retroperitoneal space infections and against E. coli, Enterobacter and Pseudomonas. Immunoglobulin‐G (IgG) continued to increase in good and fair responders until seven days after gammaglobulin treatment. IgG and the percentage of B cells in good responders increased in parallel, although the cause of this finding could not be fully elucidated. Adverse reactions to the gammaglobulin preparation were not observed in any patients.
Epidemiology of Preeclampsia and Eclampsia
Authors: Dhall Kamala
Published: August 2010

The epidemiological characteristics of 1107 cases of preeclampsia and eclampsia admitted to the Nehru Hospital, Postgraduate Institute of Medical Education and Research, Chandigarh, India, have been analysed. The incidence of preeclampsia and eclampsia was found to be 10.2 and 0.6%, respectively, of all hospital deliveries. Nulliparous patients had a higher incidence than multiparous patients in all age groups. Lack of adequate antenatal supervision was a major factor in 83% of cases, largely due to the patient̂s ignorance, illiteracy and apathetic attitude. Nutritional deficiencies and weather changes did not appear to affect the incidence of toxaemia. Early diagnosis and appropriate hospital management of these cases has been emphasised.
Cervical Dilatation for Early Abortion (New Approach)
Authors: Manorama B. , Bhattacharaya Prabha , Pawade Surendra
Published: August 2010

Cervical dilatation prior to suction evacuation tried in 230 women seeking medical termination of pregnancy from 8 to 14 weeks of gestation. In 170 women, Dilex‐C and in 60 women laminaria tent was used. Only one tent of same size was inserted and kept in cervical canal for sixteen (Group‐A), twelve (Group‐B) and eight hours (Group‐C). Mean dilatation achieved in Dilex‐C and Laminaria tent in Group‐A was 10,06 mm and 10.48 mm., in GroupB it was 10.44 mm, and 11.05 mm and in Group‐C the same was 10.01 mm and 10.00 mm respectively which is very much comparable. Clinical trial of Dilex‐C showed high degree of patien's acceptance without any obvious cervical injury. None of the patients showed displacement, hour glass formation bending or breaking, nor difficulty in removal. On follow up study at the end of one month these cases clinically did not s0how any signs of pelvic, uterine or cervical infection.
Norethisterone Oenanthate as an Injectable Contraceptive in Two Treatment Schedules in Interval Subjects
Authors: Gulati Kamlesh , Mapa Manik K. , Gupta Amar Nath , Singh Padamjit M. , Devi P.K.
Published: August 2010

The efficacy, incidence of side effects and discontinuation were studied in fifty subjects who received intramuscular norethisterone oenanthate 200 mgm in two treatment schedules over a period 18 months. Subjects in Group A received the injection every 2 months during the study period whereas those in Group B received the injection at a 2 monthly interval for the first six months and every 3 months thereafter for 18 months. No pregnancy occurred in either group. Longer cycles and a higher incidence of amenorrhea were noted in the group B subjects. The bleeding pattern showed an increase in spotting episodes, in group A at the fourth injection, following which no apparent difference in the bleeding pattern was noted in the two groups. The discontinuation rate was 80% at the end of the study, 28% of the subjects having dropped out for amenorrhea, 16% for irregular bleeding and spotting and 6% for minor medical complaints.
Vaginal Reconstruction with a Sigmoid Loop in Pelvic Exenteration
Authors: Ng Heung‐Tat , Kan Yuen‐Yee , Chao Kaun‐Chong , Yuan Chiou‐Chung
Published: August 2010

In the past 15 years, 55 patients with recurrent or advanced cervical cancer underwent pelvic exenteration. Two of them had vaginal reconstruction with a sigmoid loop concomitantly performed at the time of surgery. This procedure is particularly advantageous as: (1) it is possible to fill up the pelvic cavity denuded by the exenteration to prevent intestinal herniation, (2) it restores the vaginal function, (3) it makes the pelvic examination possible following total exenteration in which the anus and vagina in the past were almost always excised and obliterated.
Uterine Malignancies Developing after a Long Term Use of lUCD's
Authors: Hsu Chien‐Tien , Roan Cheng‐Hsiung , Chang Han‐Tong , Chen Shin‐Zon , Lin Yun‐Nan , Chen Tin‐Yao
Published: August 2010

Four cases of uterine malignancies developed after use of IUCD's: 3 cases after a long term use and one case after a short time use. In Case 1, sarcoma developed 20 years after application at the exact site where the IUCD (nylon ring) was placed and well corresponded to the size and shape of the IUCD and had also invaded the myometrium. Local chronic irritation as a causative factor is strongly suggestive. In Cases 2 and 3, endometrial cancer developed 13 and 14 years after the application of a Lippes loop. It seems injudicious to interpret the occurrence solely as an accidental coincidence. Loops after prolonged use could have played some role in the development of the endometrial cancer. In the fourth case endometrial cancer developed 3 months after the placing of a nylon ring in the uterus at the time of tuboplasty to permit easy extraction of the polyethylene tube which was inserted through the fallopian tube. In this case, in view of her age, it is possible that preexisting endometrial hyperplasia with undetected precancerous lesions might have been present when the polyethylene tube and nylon ring were inserted and subsequently evolved into malignancy. The authors emphasize the importance of awareness of malignancy in prolonged vaginal bleeding in IUCD users, and consider a short term and interrupted use of IUCD's safer and advisable, although any cause and effect relationship between IUCD and uterine malignancy is still a matter of pure conjecture.
Relationship between Prostaglandin F and Steroids in Human Peripheral Venous Plasma and Concentration of Prostaglandin F in Corpus Luteum
Authors: Tanaka Shoichi , Hata Hiroshi , Shimoya Yasuharu , Yamamoto Hiroshi , Yamamoto Hiroshi , Hashimoto Masayoshi
Published: August 2010

Concentrations of prostaglandin F and steroids in plasma and in corpus luteum (CL) were studied by radioimmunoassay during the luteal phase of the menstrual cycle in thirteen normal women.

The average concentration of PGF in corpus luteum was 4.19 ± 1.59 ng/g (mean ± SE) (ranging from 0.03 to 20.5 ng/g) and in plasma was 208.1 ± 34.7 pg/ml (ranging from 47.0 to 393.3 pg/ml). There was no correlation between the concentrations of PGF in plasma and in CL.

The average concentrations of progesterone and estradiol in plasma were 17.6 ± 1.2 ng/ml and 181.7 ± 20.7 pg/ml, respectively. These was no correlation between the plasma concentrations of PGF and steroids.

There was a significant increase (p>0.01) in the mean concentration of PGF from the follicular phase (199.5 ± 26.4 pg/ml, n=35) to the luteal phase (241.9 ± 12.9 pg/ml, n=44).

These findings are discussed in relation to the possible role of prostaglandin F in corpus luteum regression.
Danazol (an Antigonadotropin) in the Treatment of Catamenial Pneumothorax
Authors: Uemura Tsuguo , JVIatsuyama Akemi , Minaguchi Hiroshi , Ikeda Hirotada
Published: August 2010

Two cases of catamenial pneumothorax treated with danazol, an isoxazole derivative of 17α‐ethinyl testosterone, are reported. The first patient, a 44‐year‐old woman who had had 31 attacks of spontaneous pneumothorax at the onset of menstruation in the past 7 years, was given 400 mg danazol daily for 6 months. Episodes did not reoccur during the treatment or for 5 months after the treatment, though ovulation was observed in the first menstrual cycle after the treatment. The second patient was a 33‐year‐old woman who had had recurrent right‐sided chest pains related to the onset of menses. The endometrial implants in the diaphragm were excised but a right pneumothorax recurred at the first menstrual period after operation. After 4 months of therapy with danazol she has been asymptomatic for 20 months. Danazol therapy seems to be more effective on this disease and to have less side effects than other ovulation inhibiting drugs.
A Comparative Study on the NAD Glycohydrolase of the Maternal and Neonatal Erythrocytes
Authors: Suh Byung Hee , Lee Jae Hyun , Cho Yong Ho
Published: August 2010

The subcellular localization of NADase in the ghost fraction (96%) of neonatal erythrocytes was localized more than in the maternal ghost fraction (83%). The inhibition by free nicotinamide of hydrolytic activity of NADase was found to be greater (approximately twice) in the neonatal erythrocytes than in the maternal erythrocytes. The activatory effect was exerted on the both maternal and neonatal ghost‐NADase by the addition of lower concentration (less than 0.6 M) of urea. The membraneous protein of erythrocytes was fractionated into 5 bands namely, band I, II, III, IV, V and VI in the case of maternal erythrocytes in good contrast of 7 bands for the neonatal erythrocytes namely, I, II, III, IV, V, VI and VII. From these results, it was suggested that there is a definite difference between membraneous protein of maternal and neonatal erythrocytes and NAD glycohydrolase activity and it is reasoned to be due to the independent erythropoietic site for two individuals. The erythrocytes NAD glycohydrolase might be one of the isozyme which change their properties during developmental maturation. The possible functions of erythrocytes NAD glycohydrolase were also discussed with regard to erythrocytes maturation in hematopoietic system.
Uterine Malignancy Developing After a Long Term Use of IUCD Additional Report of a Case: Association of Endometrial Stromal Sarcoma with IUCD
Authors: Yang Li‐Chuan , Hsu Mei‐Lien , Jong Hong‐Lin , Wu Chii‐Ming , Lin Yun‐Nan , Chang Chung‐Chuan , Hsu Chien‐Tien
Published: August 2010

A case of endometrial stromal sarcoma associated with the use of an IUCD is to be added to our previous report which included 4 cases of uterine malignancies developing after long‐term use of IUCD. In the present case, the IUCD (Lippes loop and nylon ring) had been used for 6 years with interruptions of 1–6 months. When the IUCD is associated with irregular bleeding, it is important to emphasize that besides irregular bleeding as a complication of IUCD, abortion, ectopic pregnancies and uterine malignancies should also be taken into consideration. Endometrial stromal sarcoma is a very rare uterine malignancy, therefore, the association of this tumor with IUCD must count as a great rarity. A case of this kind has never before appeared in the literature.
Hysterosalpingography 1. The Incidence of Tubal Spasm During Hysterosalpingography
Authors: Anandakumar C. , Rauff Mary , Wong Eric , Wong P.C. , MBBS SS Ratnam
Published: August 2010

The main objective of this study was to assess the frequency of tubal spasm during hysterosalpingography. 19 patients were subjected to hysterosalpingogram in whom no pelvic pathology was detected in laparoscopic hydrotubation. Six of these patients in whom both tubes were found to be patient on laparoscopic hydrotubation showed varying degrees of tubal blockage on hysterosalpingography. The incidence of tubal spasm as a cause of tubal blockage was 32%.
A Clinico–Pathological Diagnosis of Hypertensive Disorders of Pregnancy
Authors: Dhall Kamala , Dhall Gian I. , Chugh Kirpal S. , Dutta Brajendera N.
Published: August 2010

Clinico‐pathological correlation was carried out in 25 and 65 cases of normal and hypertensive disorders of pregnancy respectively. It was observed that blood urea levels were elevated in the presence of severe glomerular lesion and serum uric acid levels had direct correlation with the magnitude of the glomerular change as well as the clinical severity of the disease. Renal histology in toxaemia when viewed by light microscopy is placed in the non‐specific category of acute membranous glomerulo nephritis and does not help much to differentiate the hypertensive disorders of pregnancy from each other. The clinical diagnosis of preeclamptic toxaemia (PET) was misleading in 21.4% of multi‐gravidae and 5.2% of primigravidae. It was felt that clinical presentation of the individual case, her previous history coupled with renal function test give the maximum help in arriving at the diagnosis.
Ultrasonographic Identification of Polycystic Ovary
Authors: Matsunaga Isao , Hata Toshiyuki , Kitao Manabu
Published: August 2010

Eleven patients with clinical findings of suspected polycystic ovary syndrome (PCOS) were examined using real‐time ultrasound. The ovarian area of PCOS (average value 488 ± 183 mm2) was larger than that of patients without PCOS (average value 184 ± 66 mm2) (P>0.001). Ovarian structures in those with PCOS were evident in the form of peripheral or general cystic patterns on the ultrasonograms. Ultrasonography proved to be a pertinent diagnostic tool for a non‐invasive diagnosis of PCOS.
Choriocarcinoma Coexisting with Adenocarcinoma in the Pars Interstitialis of the Oviduct:A Case Report
Authors: Takagi Minoru , Sato Yasuro , Cho Renchu , Koh Kabu , Nagayama Takahisa
Published: August 2010

Synopsis

Double cancer, especially choriocarcinoma and adenocarcinoma, is rarely seen. Choriocarcinoma coexisting with adenocarcinoma in the interstitial part of the oviduct was found in a 45‐year‐old Japanese woman. She was initially treated for ectopic pregnancy. On the 16th postoperative day, however, she suddenly fell into a comatose state and died from a brain hemorrhage caused by metastasis of the choriocarcinoma.

This is the first reported case of a rare combination of these two carcinomas.
A New in vitro Assay System for Prolactin‐Releasing Activity and its Application for Demonstration of Porcine Hypothalamic Prolactin‐Releasing Factor (PRF)
Authors: Nomura Mitsuo , Miyamoto Kaoru , Hasegawa Yoshihisa , Ibuki Yoshito , Igarashi Masao
Published: August 2010

A new sensitive and reproducible assay system for prolactin‐releasing activity (PRA) using a rat pituitary cell culture system was established. After pretreatment with bromocriptine (1.3 times 10‐7 M) in culture medium for 24 hr on the 4th day in culture, the 6 hr addition of thyrotropin releasing hormone (TRH) to culture medium increased prolactin secretion in a dose‐dependent manner at concentrations of TRH ranging from 3.2 times 10‐11 M to 2.0 times 10‐9 M; maximal prolactin response was 270% of the control value.

By using this new assay system for PRA, prolactin‐releasing factor (PRF) in porcine hypothalami was isolated and identified. On Sephadex G‐25 gel‐chromatography of acid extracts of porcine hypothalami, PRA was found in the small peptide portion. These fractions of PRA were further subjected to CM‐cellulose (CM‐52) ion exchange chromatography, followed by reverse‐phase HPLC. PRA obtained by HPLC chromatography was neutralized by anti‐TRH antibody. It was concluded that TRH is the main PRF in porcine hypothalamic extracts.
Midtrimester Artificial Abortion Using 16, 16‐dimeth‐l‐trans‐Δ2‐PGE1 meth‐l ester (Preglandin®), Laminaria Tents and Continuous Epidural Anesthesia
Authors: Fuchi Isao , Noda Kiichiro
Published: August 2010

For this stud‐, Preglandin (16, 16 dimeth‐l trans‐Δ2‐PGE1 meth‐l ester) was used for midtrimester artificial abortion. The methodolog‐, however, differed from that described in other reports. Our present method includes first inserting laminaria tents to dilate the cervix and administering albumin tannate to prevent side effects. We then insert PGEj vaginal suppositories to induce artificial abortion and use epidural anesthesia to relieve pain.

A total of 19 patients were used for this stud‐. In 15 patients, the abortion was performable before 5 or less PGE1 vaginal suppositories had been used. The remaining 4 patients did not reach this stage until after intravenous drip PGF2α had been administered the following da‐. PGE1 is simple to use and appears to be effective in midtrimester artificial abortion if attention is given to the possibilit‐ of bag rupture, which ma‐ occur during the earl‐ stage of the treatment.
The Relation of Pregnanc‐ Associated Plasma Protein A (PAPP‐A) in the Umbilical Circulation of the Human Fetus to Oestriol Production b‐ the Placenta
Authors: Grudzinskas J. G. , Obiekwe B. C. , Perry L. A. , Houghton D. J. , Sinosich M. J. , Bolton A. E. , Chard T.
Published: August 2010

Levels of pregnanc‐associated plasma protein A (PAPP‐A), alphafetoprotein (AFP), unconjugated oestriol (E3) and placental lactogen (hPL) were measured in maternal peripheral and umbilical cord arterial and venous blood obtained from 29 normal pregnancies at term deliver‐. PAPP‐A levels were three orders of magnitude higher in the mother than the fetus. Levels were higher in the umbilical arter‐ than the umbilical vein though this was not statisticall‐ significant. There was a close correlation between maternal and fetal levels of E3. There was no direct correlation between: (i) umbilical and maternal PAPP‐A levels; (ii) maternal PAPP‐A and E3 levels; or (iii) umbilical PAPP‐A and E3 levels. There was a significant inverse relationship between fetal PAPP‐A levels and the umbilical venoarterial difference of E3. No such relationship was found with fetal levels of AFP or hPL. These findings suggest a close functional association between PAPP‐A and the placental s‐nthesis of oestrogens.
Mixed Mesodermal Tumor of the Uterine Cervix ‐A Trial Search for Intermediate Forms between Carcinoma and Sarcoma ‐
Authors: Tokunaga Tatsu‐a , Matsu‐ama Shigemaro , Kuwahara Shozaburo , Himeno R‐uichi , Terao Kiminari
Published: August 2010

Mixed mesodermal tumor of the uterus are not uncommon; however, their histogenesis has remained unsolved.

A case of endocervical mixed mesodermal tumor consisting of a well‐differentiated squamous carcinoma and a rhabdom‐osarcoma was studied histologicall‐. The sections were stained b‐ specific keratin staining methods, Martinotti's keratin staining and the peroxidase‐antibod‐ method, as well as b‐ the usual histologic stainings. The specific stainirigs revealed keratin‐like substances within the c‐toplasm of the squamous cartinoma cells and also that of a few sarcomatous cells scattered in the area of the sarcoma. These cells, which were functionall‐ epithelial and morphologicall‐ non‐epithelial, seemed to be intermediate forms between carcinoma and sarcoma.

This finding suggested that the carcinomatous lesion might have undergone transformation into the sarcomatous one. The authors therefore believe that mixed mesodermal tumor originates from a single strain of endometrial or endocervical cells.
A Prospective Study of the Metabolic Effects of a Low Dose Combined Oral Contraceptive
Authors: Liew Diana F.M. , Ng Charles S.A. , Yong Y.M. , Ratnam S.S.
Published: August 2010

The effects of a low dose combined oral contraceptive (OC) containing 30 μg ethinyl estradiol and 150 μg levonorgestrel, on carbohydrate and lipid metabolism were studied in 39 healthy women. An oral glucose tolerance test (OGGT) was performed before and 6 cycles after OC. Glucose tolerance and insulin response were impaired. There was no change in cholesterol levels. However, triglycerides were significantly lowered but this could be due to the “carry‐over” effects of pregnancy. Body weight was also significantly decreased but was not related to triglyceride change. There was no change in blood pressure after 6 cycles of OC.
Evaluation of Estetrol Metabolism in the Fetoplacental Unit after DHAS Administration to Pregnant Women Near Term
Authors: Sagara Yusuke , Okatani Yuji , Yamanaka Satoshi
Published: August 2010

To assess the significance of measurement of plasma estetrol after dehydro‐epiandrosterone sulfate (DHAS), 100 mg of DHAS was injected 30–60 minutes (7 cases), 120 minutes (6 cases), 180 ‐ 240 minutes (5 cases) before delivery into 18 normal obstetric patients (37–39w) undergoing elective repeat cesarean section. At delivery, maternal peripheral vein (MV), umbilical vein (UV), and umbilical artery (UA) blood and amniotic fluid (AF) were obtained, and unconjugated estetrol in each compartment was measured.

A significant increase of plasma estetrol concentration was observed in MV, UV, and UA, with the maximal levels at 180–240 minutes, but not in AF. The increase of plasma estetrol concentrations were higher in UV and UA than in MV. However, no significant difference in the plasma estetrol concentration was seen between UV and UA after DHAS injection. A significant correlation was found between individual MV and UV plasma estetrol concentrations after DHAS injection.

The data indicate that determination of a maternal plasma estetrol rise after DHAS injection reflects fetoplacental function and may provide further useful information to evaluate fetal well being.