bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Domain Antibody Multimers for Detection of Botulinum Neurotoxin Serotypes C, D, and Their Mosaics in Endopep-MS
Authors: Harmsen Michiel M. , Cornelissen Jan C. , van der Wal Fimme J. , Bergervoet Jan H. W. , Koene Miriam
Published: September 2023

Botulinum neurotoxins (BoNTs) are highly toxic proteins that require high-affinity immunocapture reagents for use in endopeptidase-based assays. Here, 30 novel and 2 earlier published llama single-domain antibodies (VHHs) against the veterinary-relevant BoNT serotypes C and D were yeast-produced. These VHHs recognized 10 independent antigenic sites, and many cross-reacted with the BoNT/DC and CD mosaic variants. As VHHs are highly suitable for genetically linking to increase antigen-binding affinity, 52 VHH multimers were produced and their affinity for BoNT/C, D, DC, and CD was determined. A selection of 15 multimers with high affinity (KD < 0.1 nM) was further shown to be resilient to a high salt wash that is used for samples from complex matrices and bound native BoNTs from culture supernatants as shown by Endopep-MS. High-affinity multimers suitable for further development of a highly sensitive Endopep-MS assay include four multimers that bind both BoNT/D and CD with KD of 14–99 pM, one multimer for BoNT/DC (65 pM) that also binds BoNT/C (75 pM), and seven multimers for BoNT/C (<1–19 pM), six of which also bind BoNT/DC with lower affinity (93–508 pM). In addition to application in diagnostic tests, these VHHs could be used for the development of novel therapeutics for animals or humans.
High Diversity and Transmission Dynamics of HIV-1 Non-C Subtypes in Bangladesh
Authors: Sarker Md. Safiullah , Jahan Rubiyat
Published: September 2023

Genetic diversity and molecular epidemiology of HIV are directly relevant to HIV transmission. We report here the genetic diversity and transmission dynamics of non-C subtypes of HIV-1 strains detected in Bangladeshi key populations. Sequence analysis of gag gene revealed four subtypes A1, B, D, G, and nine CRFs (01_AE, 02_AG, 09_cpx, 10_CD, 15_AE/B, 13_cpx, 14_BG, 22_01_A1, and 25_AGU). Most of these non-C strains were detected in returnee migrant workers from different parts of the world. Phylogenetic analysis showed that the Bangladeshi HIV-1 strains detected in migrant workers and their wives and local sex workers shared common ancestries. The identification of the multiple subtypes indicates high diversity of non-C HIV-1 variants circulating in Bangladesh which might have been imported by migrant workers from multiple geographical areas.
Enhanced IL-17 Producing and Maintained Cytolytic Effector Functions of Gut Mucosal CD161+CD8+ T Cells in SIV-Infected Rhesus Macaques
Authors: Thirugnanam Siva , Walker Edith , Schiro Faith , Aye Pyone , Rappaport Jay , Rout Namita
Published: September 2023

Previous studies have indicated that the loss of CD161-expressing CD4+ Th17 cells is linked to the progression of chronic HIV. These cells are significantly depleted in peripheral blood and gut mucosa of HIV-infected individuals, contributing to inflammation and disruption of the gut barrier. However, the impact of HIV infection on CD161-expressing CD8+ T cells remain unclear. Here, we examined the functions of peripheral blood and mucosal CD161+CD8+ T cells in the macaque model of HIV infection. In contrast to the significant loss of CD161+CD4+ T cells, CD161+CD8+ T cell frequencies were maintained in blood and gut during chronic SIV infection. Furthermore, gut CD161+CD8+ T cells displayed greater IL-17 production and maintained Th1-type and cytolytic functions, contrary to impaired IL-17 and granzyme-B production in CD161+CD4+ T cells of SIV-infected macaques. These results suggest that augmented Th17-type effector functions of CD161+CD8+ T cells during SIV infection is a likely mechanism to compensate for the sustained loss of gut mucosal Th17 cells. Targeting the cytokine and cytolytic effector functions of CD161+CD8+ T cells in the preclinical setting of chronic SIV infection with antiretroviral therapy has implications in the restoration of gut barrier disruption in persons with HIV infection.
Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains
Authors: Ruan Shengnan , Ren Wenhui , Yu Bin , Yu Xuexiang , Wu Hao , Li Wentao , Jiang Yunbo , He Qigai
Published: September 2023

Background: Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome (PRRS), leading to abortion in sows and respiratory distress in breeding pigs. In China, PRRSV1 and PRRSV2 are the two circulating genotypes in swine herds, with distinct virulence. PRRSV2 further consists of classical (C-PRRSV2), highly pathogenic (HP-PRRSV2), and NADC30-Like (N-PRRSV2) subtypes. The diversity of PRRSV poses challenges for control and eradication, necessitating reliable detection assays for differentiating PRRSV genotypes. Methods: A new TaqMan-based RT-qPCR assay with four sets of primers and probes targeting conserved regions of the ORF7 and NSP2 genes of PRRSV was developed, optimized, and evaluated by us. Reaction conditions such as annealing temperature, primer concentration, and probe concentration were optimized for the assay. Specificity, sensitivity, repeatability, stability, limit of detection (LOD), concordance with the reference method were evaluated for the assay. Results: The assay could detect and type PRRSV1, C-PRRSV2, HP-PRRSV2, and N-PRRSV2 simultaneously with 97.33% specificity, 96.00% sensitivity, 12 copies/μL LOD, 97.00% concordance with reference assays. We applied the assay to 321 clinical samples from swine farms in China. The assay successfully detected and typed 230 PRRSV-positive samples, with 24.78% (57/230) of them further confirmed by ORF5 gene sequencing. The prevalence of PRRSV subtypes among the positive samples was as follows: C-PRRSV2 (15.22%), HP-PRRSV2 (23.48%), and N-PRRSV2 (61.30%). Two samples showed coinfection with different PRRSV subtypes. Conclusion: The quadruple RT-qPCR assay is a powerful tool for detecting and typing the currently circulating PRRSV strains in Chinese swine populations. It can assist in the surveillance of PRRSV prevalence and the implementation of prevention and control strategies.
Beta-Adrenergic Agonists, Dietary Protein, and Rumen Bacterial Community Interactions in Beef Cattle: A Review
Authors: Pfau Alison P. , Shepherd Elizabeth A. , Martin M. Gabbi , Ascolese Sophia , Mason Katie M. , Egert-McLean Amanda M. , Voy Brynn H. , Myer Phillip R.
Published: September 2023

Improving beef production efficiency, sustainability, and food security is crucial for meeting the growing global demand for beef while minimizing environmental impact, conserving resources, ensuring economic viability, and promoting animal welfare. Beta-adrenergic agonists and dietary protein have been critical factors in beef cattle production. Beta-agonists enhance growth, improve feed efficiency, and influence carcass composition, while dietary protein provides the necessary nutrients for muscle development and overall health. A balanced approach to their use and incorporation into cattle diets can lead to more efficient and sustainable beef production. However, microbiome technologies play an increasingly important role in beef cattle production, particularly by optimizing rumen fermentation, enhancing nutrient utilization, supporting gut health, and enhancing feed efficiency. Therefore, optimizing rumen fermentation, diet, and growth-promoting technologies has the potential to increase energy capture and improve performance. This review addresses the interactions among beta-adrenergic agonists, protein level and source, and the ruminal microbiome. By adopting innovative technologies, sustainable practices, and responsible management strategies, the beef industry can contribute to a more secure and sustainable food future. Continued research and development in this field can lead to innovative solutions that benefit both producers and the environment.
Comparative Analysis of the Hydrodynamic Performance of Dual Flapping Foils with In-Phase and Out-of-Phase Oscillations
Authors: Hua Ertian , Qiu Linfeng , Xie Rongsheng , Su Zhongxin , Zhu Wenchao
Published: September 2023

In the context of the plain river network, conventional water pumps suffer several drawbacks, including inadequate efficiency, poor security, and costly installation costs. In order to improve the hydrodynamic insufficiency problem and enhance the hydrodynamic performance and applicability of flapping hydrofoils, this paper proposes a bionic pumping device based on dual flapping foils. Based on the finite volume method and overlapping grid technology, the numerical simulation and experimental verification of the hydraulic performance of two typical motion modes of in-phase and out-of-phase oscillations are conducted, thereby providing a theoretical foundation for improving and optimizing the design of flapping hydrofoils. The results show that the out-of-phase oscillation has better hydraulic performance compared to the in-phase oscillation. The formation of the tail vortex structure plays a crucial role in determining the hydraulic efficiency of dual flapping foils, with in-phase oscillation forming a pair of vortex streets and out-of-phase oscillation forming two pairs of vortex streets. The pumping efficiency of the out-of-phase oscillation is significantly higher than that of the in-phase oscillation, reaching up to 38.4% at a fixed frequency of f = 1 Hz, which is an increase of 90.5% compared to the in-phase oscillation. The characteristic curve of the in-phase oscillation shows an “S” type unstable oscillation phenomenon, namely the hump phenomenon, while the out-of-phase oscillation does not show such a phenomenon, which can effectively expand its application range. In addition, the applicable head of the out-of-phase oscillation hydrofoil is lower, which can better meet the requirements of ultra-low head conditions.
The Impact of Land—Use Composition and Landscape Pattern on Water Quality at Different Spatial Scales in the Dan River Basin, Qin Ling Mountains
Authors: Zhang Yuanyuan , Zhao Yan , Zhang Huiwen , Cao Jing , Chen Jingshu , Su Cuicui , Chen Yiping
Published: September 2023

To study the impact of land—use structure and landscape pattern on water quality at different spatial scales in the Dan River Basin (Qin Ling Mountains, China), water samples from 21 sites along the Dan River were collected in 2022 during the dry and wet seasons, and nine water quality indices were tested. Land—use composition and landscape pattern indices at riverine reach, riparian, and sub—basin were obtained, and correlation analysis and redundancy analysis (RDA) were used to determine the relationship with water quality. The results are as follows. (1) Water quality in the Dan River is better in the wet season than in the dry season; the main pollutants are total nitrogen (TN) and total phosphorus (TP). (2) The impact of land—use composition and landscape pattern on water quality has a scale effect; riverine reach can best explain the water quality. (3) Agricultural land and forest have the greatest impacts on water quality; agricultural land and construction land aggravate the deterioration of water quality, while forest, grassland, and water area have positive effects on water quality. The largest patch index (LPI) and contagion index (CONTAG) were positively correlated with pollutants, while Patch richness density (PRD), Patch shape (PD), Shannon’s diversity index (SHDI), and landscape shape index (LSI) were negatively correlated with pollutants, indicating that with an increase in the impact of human activities on landscapes, the degree of fragmentation decreases patch richness, landscape shape tends to be simplified, and water pollution is eventually aggravated. Land planners should focus on optimizing the land—use structure and landscape pattern to increase the diversity of the landscape. Therefore, strict environmental regulations must be established.
Unsaturated Vertical Flow Constructed Wetland for Chlorothalonil Remediation with Target Application in Ethiopian Floriculture Industry
Authors: Wehbe Stan , Zewge Feleke , Inagaki Yoshihiko , Sievert Wolfram , Nutakki Tirumala Uday Kumar , Deshpande Akshay
Published: September 2023

The Ethiopian floriculture industry produces large amounts of wastewater, which requires treatment systems with lower retention times, higher hydraulic flow, and higher hydraulic loading rates (HLRs). Unsaturated vertical flow constructed wetlands (UVF-CWs), which represent these characteristics, have not been studied in depth for chlorothalonil remediation from wastewater. In this study, six UVF-CWs and nine pumping stations were organized into three experimental groups as three independent two-stage CW systems. The influent was pumped into the first vertical-flow stage, after which the effluent was collected and pumped into the second vertical-flow stage. The stage A and stage B effluents were tested for chlorothalonil removal at three different HLR of 50, 200, and 400 L d−1 m−2 and two influent chlorothalonil concentrations of 100 and 500 µg L−1. The chlorothalonil levels for the stage A effluent at an HLR of 50 L d−1 m−2 for both influent chlorothalonil concentrations were below the detection limit of 0.08 µg L−1. A maximum chlorothalonil concentration of 7.9 and 196 µg L−1 was observed in the stage A effluent for influent concentrations of 100 and 500 µg L−1, respectively. The chlorothalonil levels for the stage B effluent were all below the detection limit of 0.08 µg L−1. A final chlorothalonil removal efficiency of at least 99.9% was observed for both influent chlorothalonil concentrations at the three HLRs used. These results demonstrated that UVF-CWs represent a viable solution for chlorothalonil remediation in the Ethiopian floriculture industry.
On the Usefulness of the Proper Orthogonal Decomposition on the Description of the Highly Concentrated Sediment Release Phenomena Resulting from a Two-Phase Solid-Fluid Simulation: Effect of the Ambient Current
Authors: Santa Cruz Alina , Nguyen Duc Hau , Guillou Sylvain S.
Published: September 2023

Proper orthogonal decomposition (POD) is used to examine the release of highly concentrated water–sediment mixture in water, with or without ambient current. This technique allows us to extract the dominant features in spatio-temporal data sets and the POD total energies associated to the base parameter of the decomposition. Both one-component and two-component POD techniques are, respectively, applied on data relative to the solid volume fraction and on the solid volume fraction velocities. The analysis is based on an experiment in the literature and data sets provided by a two-phase flow solid-fluid numerical simulation. For release phenomenon without ambient current, the analysis of the POD results highlights that the impact of the particle diameter on the solid phase dynamics and the particle dispersion is modest during the falling time, but that it becomes preponderant during the formation of a turbidity current. Aided by POD, the impact of the ambient current is studied for a given particle diameter. As the ambient current becomes strong, we can observe the effect of the resistance of the bottom against the water–sediment mixture transport. According to the strength of the ambient current, the POD results show that the dynamics of the release phenomenon have two different regimes on either side of a clearly identified threshold value.
Heavy Metal Contamination in Surface Water of Harike Wetland, India: Source and Health Risk Assessment
Authors: Naqash Nafiaah , Jamal Mamdoh T. , Singh Rahul
Published: September 2023

Amidst industrialization and urbanization, wetlands face pollution challenges. We investigated the seasonal distribution of five heavy metals (As, Cd, Cr, Pb, and Zn) in the surface water of Harike wetland. The surface water samples were collected from four different stations selected along Harike wetland. Our results indicate As, Cr, and Zn levels were within WHO standards, however, Cd in winter (7.07 µg/L), monsoon (4.45 µg/L), and post-monsoon seasons (3.13 µg/L) exceeded the limits. Pb surpassed the standards in winter (278 µg/L) and monsoon seasons (14.5 µg/L). In winter, Pb and Cd had higher levels, and the pollution level was classified as moderate. Cd, however, was categorized under light pollution status during the monsoon and post-monsoon seasons. The health risk assessment indicated that the hazard quotient (HQ) and hazard index (HI) values for both ingestion and dermal pathways were within the safety limits (HQ < 1 and HI < 1) for both population groups (adults and children). The multivariate statistical analysis reported the correlation and further indicated different sources of heavy metals from nearby industries, agriculture, and mining. This research highlights the importance of continued monitoring and emphasizes the potential for positive environmental changes, as exemplified by the influence of the COVID-19 pandemic. These findings hold global relevance and offer valuable input for the development of precise action plans aimed at elevating water quality standards on an international scale.
Comparison of Battery Electric Vehicles and Fuel Cell Vehicles
Authors: De Wolf Daniel , Smeers Yves
Published: September 2023

In the current context of the ban on fossil fuel vehicles (diesel and petrol) adopted by several European cities, the question arises of the development of the infrastructure for the distribution of alternative energies, namely hydrogen (for fuel cell electric vehicles) and electricity (for battery electric vehicles). First, we compare the main advantages/constraints of the two alternative propulsion modes for the user. The main advantages of hydrogen vehicles are autonomy and fast recharging. The main advantages of battery-powered vehicles are the lower price and the wide availability of the electricity grid. We then review the existing studies on the deployment of new hydrogen distribution networks and compare the deployment costs of hydrogen and electricity distribution networks. Finally, we conclude with some personal conclusions on the benefits of developing both modes and ideas for future studies on the subject.
Adaptation of the One Health Zoonotic Disease Prioritization Tool for Government and Privately Owned Companion Animal Zoonotic Disease Surveillance
Authors: Bayko Heather , Watkins Sarah , Waugh Sheldon , Moore Gerald , Mullaney Sara B.
Published: September 2023

The U.S. Army Veterinary Services (AVS) provides public health guidance, consultation, and clinical support regarding zoonoses for the Department of Defense (DoD). AVS One Health Division was tasked with developing a surveillance tool for zoonoses of companion animals presenting to DoD veterinary facilities. Such a tool could help monitor the spread of zoonoses between U.S. military Service Members, their family members, and their pets. The primary objective was to prioritize zoonoses of interest for companion animal disease surveillance in the DoD. AVS implemented a semi-quantitative One Health approach to prioritize zoonoses of interest. The prioritization process followed five steps: (1) generate list of zoonoses to be ranked that are applicable to a DoD companion animal disease surveillance system, (2) develop criteria to identify the importance of a zoonoses, (3) develop criteria definition questions, (4) rank criteria, and (5) rank zoonoses. The prioritization process resulted in a ranked list of 14 zoonoses of interest which was presented to AVS leadership with three potential courses of action. Twelve zoonoses were selected for inclusion in DoD Companion Animal Disease Surveillance. The prioritized list of diseases was the first step in developing a DoD companion animal disease surveillance effort. Mirroring such an approach in civilian companion animal populations could fill a critical public health gap.
La unidad física como contrapartida de la unidad monetaria
Authors: Feria Rodríguez José Janier
Published: September 2023

Objetivo: Mostrar la teoría Física Monetaria, desarrollada a partir de la contabilidad llevada por un grupo de Cooperativas en la Habana. Método: Empírico (observación) y Teórico (lógico). Se analizó el trabajo de 29 cooperativas, observando las unidades que se medían, cómo se registraban estas mediciones y cuáles eran los reportes que se obtenían. Resultados: Nació la unidad Física, la cuenta física, y El Brédito como nueva dimensión contable, conceptos que se integraron con la creación de las Leyes de las Dicotomías Contables y de una nueva Ecuación. Conclusión: La teoría emergió como un fuerte instrumento de control, registro contable, medición y análisis de la realidad. La unidad física es una adecuada contrapartida de la unidad monetaria.
Konsultacje projektów rekomendacji prowadzone przez Komisję Nadzoru Finansowego – aspekty prawnounijne
Authors: Śliwa-Wajda Magdalena , Wajda Paweł
Published: September 2023

Niniejszy artykuł jest poświęcony analizie prawnej instytucji konsultacji projektów rekomendacji Komisji Nadzoru Finansowego1 dla rynku ubezpieczeniowego, o której mowa w art. 365 ust. 2 ustawy z dnia 11 września 2015 roku o działalności ubezpieczeniowej i reasekuracyjnej (tekst jedn.: Dz. U. 2022, poz. 2283, z późn. zm.; dalej: u.dz.u.r.) w świetle regulacji i standardów unijnych. Przepis ten został wprowadzony do polskiego porządku prawnego jako postać implementacji przepisu z art. 71 ust. 2 dyrektywy Solvency II2 , jednak prawidłowość dokonania tej implementacji budzi poważne wątpliwości. Dlatego też celem niniejszego opracowania jest ocena prawidłowości implementacji instytucji konsultacji projektów aktów typu soft law dla rynku ubezpieczeniowego do polskiego porządku prawnego, w szczególności zaś odpowiedź na pytanie badawcze: czy konsultacje przewidziane w art. 365 ust. 2 u.dz.u.r. spełniają wynikające z dyrektywy Solvency II wymogi co otwartości, przejrzystości i proporcjonalności?
Odpowiedzialność cywilna podmiotów rolniczego handlu detalicznego i możliwości jej ubezpieczenia
Authors: Walczak Damian , Ziemiak Michał
Published: September 2023

Troska o środowisko jest coraz istotniejsza. Do 2050 roku Europa ma się stać kontynentem neutralnym dla klimatu za sprawą wdrożenia programu Europejski Zielony Ład. Jedną z inicjatyw w ramach Zielonego Ładu jest działanie od pola – w gospodarstwie rolnym – do stołu – w domu konsumenta. Ważnym elementem rozwoju idei „od pola do stołu” w Polsce jest rolniczy handel detaliczny (RHD). Celem rozważań podjętych w niniejszym artykule jest próba odpowiedzi na następujące pytania: 1) na jakich zasadach rolnicy prowadzący rolniczy handel detaliczny ponoszą odpowiedzialność cywilną wobec klientów oraz 2) czy RHD może być objęty odpowiedzialnością ubezpieczeniową (a jeśli tak, to w ramach jakich ubezpieczeń i w jakim zakresie). W pracy analizie poddane zostaną zasady odpowiedzialności (cywilnej i ubezpieczeniowej) oraz ryzyka związane z odpowiedzialnością za produkt tworzony w ramach rolniczego handlu detalicznego. Analizie nie będą natomiast poddane np. ryzyka środowiskowe, związane z koniecznością wycofania produktu lub dotyczące utraty zysku, są to bowiem zupełnie odmienne ryzyka.
Pojęcie toczącego się postępowania sądowego oraz prawo właściwe dla oceny skutków postępowania upadłościowego na toczące się postępowanie sądowe w rozumieniu art. 292 dyrektywy Wypłacalność II Glosa do wyroku Trybunału Sprawiedliwości z dnia 13 stycznia 2022 roku w sprawie C-724/20, Paget Approbois SAS przeciwko Depeyre entreprises SARL i Alpha Insurance A/S1
Authors: Wowerka Arkadiusz
Published: September 2023

Wświetle prawa krajowego wszczęcie postępowania upadłościowego skutkuje zazwyczaj zawieszeniem postępowania toczącego się przeciwko upadłemu dłużnikowi, które zostało wszczęte przed ogłoszeniem upadłości. W przypadku, gdy wobec dłużnika została ogłoszona upadłość w innym państwie niż państwo, w którym toczy się wszczęte wcześniej przeciwko niemu postępowanie sądowe w sprawie majątkowej, powstaje jednak zasadnicze pytanie o to, wedle jakiego prawa krajowego należy dokonywać oceny skutku ogłoszenia zagranicznej upadłości na to postępowanie i w jakim zakresie. Inaczej rzecz ujmując, należy rozstrzygnąć, czy zastosowanie ma prawo właściwe dla upadłości (lex concursus), czy też prawo państwa toczącego się postępowania sądowego (lex fori processus). Prawo unijne ustanawia jednolite normy kolizyjne rozstrzygające powstającą kolizję między tymi wchodzącymi rachubę systemami prawnymi. Mimo to pojawiają się wątpliwości co do szczegółowego rozgraniczenia między lex concursus a lex fori processus. Sporne może być również samo pojęcie sprawy sądowej toczącej się przeciwko upadłemu dłużnikowi. Wkomentowanym wyroku Trybunał Sprawiedliwości dokonał wykładni stosownych przepisów wtórnego prawa unijnego z związku z likwidacją zakładu ubezpieczeń objętą zakresem zastosowania dyrektywy 2009/138/WE. Na wstępie Trybunał wyjaśnił pojęcie toczących się spraw sądowych, następnie określił zakres zastosowania lex fori processus jako prawa właściwego dla skutków postępowania upadłościowego na toczące się postępowanie sądowe. W komentowanym judykacie Trybunał kontynuuje i doprecyzowuje swoje dotychczasowe orzecznictwo dotyczące upadłości transgranicznych, w szczególności w sprawie Bulstrad Vienna Insurance Group. W ocenie autora zarówno rozstrzygnięcie, jak i jego uzasadnienie zasługują na pozytywną ocenę.
CHONDROCYTES SECRETORY PHENOTYPE ASSOCIATED WITH AGING: ROLE IN THE PATHOGENESIS OF OSTEOARTHRITIS AND PROSPECTS FOR PEPTIDE BIOREGULATION
Authors: Мякишева С.Н. , Линькова Н.С. , Кожевникова Е.О. , Рыжак Г.А.
Published: September 2023

Остеоартрит (ОА) является социально значимым ассоциированным с возрастом заболеванием, для терапии которого проводится поиск новых эффективных лекарственных средств. Развитие ОА коррелирует с формированием секреторного фенотипа хондроцитов, ассоциированного со старением (SASP). Цель обзора — анализ пула сигнальных молекул, формирующих SASP хондроцитов при ОА, и обоснование возможности пептидной хондропротекции. Установлено, что SASP хондроцитов характеризуется снижением синтеза сиртуинов, нарушением ремоделирования межклеточного матрикса и активацией продукции цитокинов. Сигумир, полипептидный комплекс хрящевой и костной тканей молодых животных, и трипептид AED (Карталакс) показали высокую эффективность в моделях ОА на животных и при пероральном применении у пациентов старших возрастных групп с OA. Эти пептидные вещества регулируют синтез проапоптозных и пролиферотропных молекул, формирующих SASP хондроцитов.

Osteoarthritis (OA) is a socially signifi cant age-associated disease, for the treatment of which a search for new effective drugs is underway. The development of OA correlates with the development of the aging-associated secretory chondrocyte phenotype (SASP). The purpose of the review is to analyze the pool of signaling molecules that form SASP of chondrocytes in OA and substantiate the possibility of peptide chondroprotection. It has been established that SASP of chondrocytes is characterized by a decrease in the synthesis of sirtuins, impaired remodeling of the extracellular matrix, and activation of cytokine production. Sigumir, a polypeptide complex of cartilage and bone tissues of young animals, and the AED tripeptide (Kartalax) have shown high effi cacy in animal models of OA and oral administration in patients with OA of older age groups. These peptide substances regulate the synthesis of proapoptotic and proliferotropic molecules that form the SASP of chondrocytes.
A MODEL FOR ASSESSING AND CORRECTING THE RISK OF DEVELOPING PRESSURE ULCERS IN THE SYSTEM OF LONG-TERM CARE FOR GERIATRIC PATIENTS WITH LIMITED MOBILITY
Authors: Башкирёва А.С. , Колосова Г.В. , Баранова Н.П. , Богданова Д.Ю. , Бондаренко Т.В. , Шишко А.В. , Асатрян А.Г. , Аникеев П.П.
Published: September 2023

Статья посвящена результатам третьего этапа научно-практического проекта по внедрению передовых социальных практик на основе инновационных геронтотехнологий в систему долговременного ухода за пациентами гериатрического профиля. Установлено, что старческий ксероз, или «хрупкость» кожи, встречается у 75 % населения старше 65 лет, когда повышенная сухость кожи приводит к шелушению, формированию микротрещин, появлению сенильного зуда, нарушений сна, развитию пролежней и, как следствие, снижению двигательной активности. В ходе проекта была выработана тактика ухода за «хрупкой» кожей у пациентов со старческим ксерозом и риском развития пролежней. В статье представлены сведения об актуальных проблемах диагностики, коррекции и профилактики пролежней у маломобильных пациентов пожилого и старческого возраста. Изложены результаты сравнительного анализа степени выраженности пролежней и их влияния на качество жизни в системе долговременного ухода за маломобильными пациентами гериатрического профиля до и после применения эмолента Cicalfate+ Eau Thermale Avène («Pierre Fabre», Франция). Построена модель и даны практические рекомендации по применению наиболее информативных и адекватных шкал для прогнозирования и оценки риска развития пролежней у пациентов старших возрастных групп.

The article is devoted to the results of the third stage of the scientifi c and practical project on the introduction of advanced social practices based on innovative gerontotechnologies into the system of long-term care for geriatric patients. It has been established that senile xerosis or skin «frailty» occurs in 75% of the population over 65 years old, when increased dryness of the skin leads to peeling, the formation of microcracks, the appearance of senile itching, sleep disturbances, the development of pressure ulcers and, as a result, a decrease in motor activity. During the project, a tactic was developed for the care of «frail» skin in patients with senile xerosis and the risk of pressure ulcers. The article presents information about the current problems of diagnosis, correction and prevention of pressure ulcers in patients with limited mobility of elderly and senile age. The results of a comparative analysis of the severity of pressure ulcers and their impact on the quality of life in the system of long-term care for geriatric patients with limited mobility before and after the use of Cicalfate+ Eau Thermale Avène («Pierre Fabre», France) emollient were presented. A model was built and practical recommendations were given on the use of the most informative and adequate scales for predicting and assessing the risk of developing pressure ulcers in patients of older age groups.
G-20 ÜLKELERİNDE JEVONS PARADOKSUNUN GEÇERLİLİĞİ ÜZERİNE PANEL VERİ ANALİZİ
Authors: KUBAR Yeşim , KAYMAZ Öznur
Published: September 2023

Gelişen teknoloji ve yenilikler ile birlikte dünya nüfusunun artması sonucu gereksinimler de artmakta bu nedenle daha fazla üretim yapmak gerekmektedir. Üretimin arttırılması ve ürünlerin kullanılması esnasında enerji gerekmektedir ve hayatın her noktasında ihtiyaç duyulan bu enerji kıt bir kaynaktır. Enerji verimliliği politikalarının etkinliği, enerji kaynaklarında dışa bağımlılığı azaltarak sürdürülebilir kalkınmaya katkı sağlamaktadır. Enerjinin eskisine nazaran verimli kullanımı enerji tasarrufunu ortaya çıkararak literatürde Rebound Etkisi olarak adlandırılmaktadır. Bu çalışma G-20 ülkelerinin 1980-2015 yıllık verilerinden faydalanarak enerji kullanımı–enerji verimliliği ilişkisini Jevons Paradoksu çerçevesinde incelenmiştir. Çalışmanın bağımlı değişkeni enerji kullanımı, bağımsız değişkenleri ise şehir nüfusu, kişi başı GSYH, ticari açıklık, enerji ithalatı, enerji yoğunluğu ve enerji tüketiminden oluşmaktadır. Çalışmada panel veri analizi kullanılmıştır. Uzun dönem tahmincisi analiz sonuçlarına göre; kişi başı GSYH meydana gelen % 1 bir birimlik artışın enerji kullanımını % 0.13 azalttığı, enerji ithalatındaki % 1 bir birimlik artışın ise enerji kullanımını yüzde 0.21 birim artırdığı belirlenmiştir. Ayrıca, enerji yoğunluğundaki % 1 bir birimlik artışın enerji kullanımını yüzde 0.012 oranında azalttığı ve şehir nüfusundaki % 1 bir birimlik artışın enerji kullanımını yüzde 0.81 birim azalttığı sonuçlarına ulaşılmıştır. Elde edilen analiz bulguları sonucu jevons paradoksu desteklenmemektedir.
ANALISIS USER INTERFACE PADA WEBSITE STAINU PACITAN MENGGUNAKAN METODE EIGHT GOLDEN RULES
Authors: Budi Susila Candra
Published: September 2023

Analysis of the user interface on the Pacitan STAINU website is a study that aims to analyze an interface on the Pacitan STAINU website. The STAINU website itself is one of the information media for lecturers and students to provide all information related to campus academic activities. The analysis used in this study uses the Eight Golden Rules or 8 golden rule principles developed by Ben Shneiderman in order to test whether the user interface on the web is in accordance with the wishes of the user. The results of the analysis found that the average recipes stated good. This is obtained based on the results of data analysis on each variable, namely for consistency variables with a value of 147.5, Shortcut Variables with a value of 161, Feedback Variables with a value of 160.5, Closure Dialogue Variable with a value of 166.5, Simple Error Handling Variables with a value of 161, Resersible Action Variables with values 157, Put User In Control variable with value 161, Reduce Variable Short Term
PERANCANGAN WIRELESS SENSOR NETWORK MENGGUNAKAN TEKNOLOGI MULTISENSOR SEBAGAI SISTEM MONITORING KUALITAS UDARA
Authors: Silvia Ade , Halimatussa’diyah2 R.A , Rizky Aldi Rahmad , Latifah Nyayu
Published: September 2023

Along with the current development, air quality is very vulnerable to being polluted. Factors that affect air quality are an increase in infrastructure development, factories,  motor vehicle fumes and any other human activity. Based on these factors, an air quality monitoring system that is integrated into a Wireless Sensor Network (WSN) system is needed. In this study, a Wireless Sensor Network will be designed using Multisensor Network technology that works in real time to measure pollutant gas levels using the TGS 2442 sensor as a measure of carbon monoxide (CO) , MG811 sensor as a measure of carbon dioxide (CO2) , TGS 2611 sensor as a measure of HydroCarbon (HC), the DHT-11 sensor as a temperature and humidity meter and the SHARPGP2Y1010 sensor as a measure of particulate levels in the air (PM10). The data from this sensor reading is sent to the server using a Raspberry pi microprocessor. Furthermore, the data will be processed until it becomes information that can be used by users or the general public.
KOTAK SAMPAH OTOMATIS BERBASIS ARDUINO MEGA 2560
Authors: Mahendra Reza , Salamah Irma , Nasron Nasron
Published: September 2023

Environmental Cleanliness still have low public attention. Public tends to inapropriately littering which caused by the trash bin is in full capacity. This things happened because the lack of monitoring by the janitor. The problem mentioned before can be overcome by designing an automatic monitoring system without being monitored all time and send notification. This research will design an automatic trash bin which will send notification of SMS upon reaching full capacity. This trash bin is equipped with Arduino Mega 2560, SIM 900 module, and infrared. This research will be implemented for a better quality of environmental protection.
UJI KELAYAKAN KUALITAS PASIR SUNGAI MAUBESI DENGAN PASIR LUMAJANG TERHADAP KUAT TEKAN BETON DAN KUAT TARIK BETON ( MUTU FC’ 25 MPa)
Authors: Setya Wijaya Handika , Tahik Blasius
Published: September 2023

Aggregate needs in Maubesi, North Central Timor Regency, , as a concrete mixture for the work of construction of natural stone, which has quite a large potential. Research is aimed at; 1) know the characteristics of the statical river in the Maubesi, North Central Timor Regency,  and aggregate  dulcet from Lumajang; 2) find to uncover of the pressure concrete and the pull that has been generated from the river  of  North East Timor Regency and the aggregate dulcet of  Lumajang; 3) find out the modulus of elasticity of the rivers of the from river in the Middle East North Coast and the Lamajang . This research is a collaborative research on the sample base and analysis of aggregate characteristics and testing of basic conditions and tests interested in and modulus of elasticity. Aggregates Strong pressure from the basement to the beginning until the end is obtained from the 2.747 walk in zone 1 ( fine send ) aggregate North Center of Central Timor, with the strength of Central Timor in the amount of 13,472 MPa ( congrete age ) silkiness of the (concrete age 7 days) , 20,727 MPa (concrete age 28 days), The compressive strength of concrete from the initial age of concrete to the end was obtained from the Maubesi aggregate of North Central Timor with compressive strength of 13,472 MPa (concrete age of 7 days), 20,727 MPa (concrete age of 28 days), and strength Tensile pull of 1,529 MPa (7 days concrete age), 2,352 MPa (28 day concrete age). While the compressive strength of concrete is obtained from the aggregate. Lumajang with compressive strength totaling 18,072 MPa (7 days concrete age), 27,802 MPa (28 days concrete age), and Tensile strength of aggregate rolled concrete. , 3,297 MPa (concrete age 28 days). Modulus of elasticity of the Maubesi North Central Timor aggregate 25677.1 N / mm² while for Lumajang aggregate 25385.9141 N / mm²
Robot Pengiriman Obat Dengan Rute Tercepat Menggunakan Algoritma Djikstra
Authors: Ilham Bagastama
Published: September 2023

ABSTRACT

The development of today's era has become a necessity where technology is one of the important factors in human life, humans always create innovations to make work easier and solve problems that always arise. In December 2019 the emergence of a pandemic caused by a virus called Covid -19 This virus can be transmitted from human to human through splashes of body fluids or contaminated surfaces. To solve this problem the author took the initiative to help nurses by designing drug distribution tools so that nurses do not need to make direct contact with patients and drug distribution will be sent more efficiently, this tool uses a TCRT line sensor which is used to identify the path then the data is sent to the Arduino microcontroller UNO to be processed to find out the fastest route using the djikstra algorithm then instructs the DC motor to move towards the cooled destination.
STUDI PERENCANAAN SALURAN DRAINASE DI JALAN SIMPANG GAJAYANA KOTA MALANG
Authors: Suhudi Suhudi , Frida Sulistyani2 Kiki , Noorvy Khaerudin3 , Dian , Sahwa Putra Dius4 Yohanes
Published: September 2023

Seiring dengan berkembangnya zaman, semakin bertambah jumlah penduduk pada suatu kawasan permukiman. Dengan banyaknya bangunan serta gedung-gedung bertingkat meyebabkan wilayah resapan air menjadi berkurang, sehingga dapat menimbulkan berbagai masalah, permasalahan tidak hanya pada sumber resapan air, namun sistem pembuangan air yang buruk juga menjadi penyebab utama sering terjadinya genangan air pada beberapa ruas jalan di Kota Malang, terutama pada ruas Jalan Simpang Gajayana. Untuk mengatasi permasalah tersebut maka perlu adanya evaluasi terkait perencanaan saluran baru dengan tujuan agar air tidak menggenangi permukaan perkerasan jalan. Penalitian ini bertujuan untuk mengetahui kondisi eksisting saluran drainase, desain dimensi saluran drainase dan efisiensi saluran. Dalam penelitian ini pertama-tama dilakukan pengumpulan data berupa data primer dan sekunder, kemudian dilakukan analisis data seperti analisis hidrologi, hidrolika dan efisiensi saluran. Dari hasil analisis dan perhitungan didapatkan Qr5 th =0,028 – 0,209 m3/dt, Qr10 th =0,029 – 0,222 m3/dt, dimensi saluran baru SG 4 KR sepanjang 74 m direncanakan dengan ukuran 60 x 60 cm, SG 7 KR dengan sepanjang saluran 131 m direncanakan dengan ukuran 40 x 40 cm dan saluran SG 3 KN dengan panjang saluran 75 m direncanakan dimensi saluran dengan ukuran 60 x 60 cm. Dari hasil perhitungan yang dilakukan didapatkan bahwa sebelum adanya saluran pada SG 4 KR,SG 7 KR dan SG 3 KN sebanyak 6 saluran pada ruas Jalan Simpang Gajayana tidak efisien dan hanya 2 saluran yang efisien, setelah dilakukan evaluasi saluran dan pembuatan saluran baru maka seluruh yang ada dinyatakan efisien dalam membuang air didalam saluran.
Pengaruh Penggunaan Limbah Abu Sekam Padi dan Viscocrete 1003 Terhadap Kuat Tekan dan Kuat Lentur Beton
Authors: Ningrum Diana , Rasidi Nawir , Siritoitet Hamdisuryaman
Published: September 2023

The purpose of this study was to determine the effect of the use of waste rice husk ash and viscocrete 1003 on the compressive strength and flexural strength of concrete with variations in the mixture of rice husk ash waste 0%, 25%, 30% and viscocrete 1003 0.6%. The results showed that the chemical content of rice husk ash that had been burned was high SiO2 (Silicon Dioxide), which was 75.5 ─ 87.9 % by weight, while the chemical content of unburned rice husk ash was SiO2 (Silicon Dioxide). Which is high that is equal to 86.9 ─ 94.7% by weight. The compressive strength test value of each variation of the specimen which is converted into 28 days of age is for variation 0% the average compressive strength value is 21.590 MPa, for variation 25% with an average compressive strength value of 15.635 MPa and 30% variation with the average compressive strength value is 11.486 MPa. So it can be concluded that the average compressive strength value from the 0% variation to the 25% variation has decreased by 0.05955%, while from the 25% variation to the 30% variation it has decreased by 0.0415%. The flexural strength test value in terms of the maximum average moment on the slab for each variation of the test object converted into 28 days of age is for 0% variation of 146.820 kN.m, for 25% variation of 165.760 Kn.m and variation of 30% of 170.490 Kn.m. So it can be concluded that the average flexural strength value from the 0% variation to the 25% variation has increased by 3.13%, while from the 25% variation to the 30% variation it has increased by 3.36%.
ANALISIS PENGARUH JUMLAH GCP (GROUND CONTROL POINT) TERHADAP AKURASI PETA ORTHOFOTO PADA JALAN YANG BERKARAKTER CURAM DENGAN TIKUNGAN TAJAM HASIL PEMOTRETAN UDARA METODE UAV-FOTOGRAMETRI
Authors: Susilo Helik , Novianti Bani Martince , Nur Fajarwati Anisah
Published: September 2023

Studi tentang pengaruh konfigurasi Ground Control Point (GCP)  terhadap akurasi peta orthofoto hasil pemotretan metode UAV-Fotogrametri telah banyak dilakukan. Akan tetapi penelitian-penelitian tersebut dilaksanakan dengan kondisi objek berupa area atau jalan yang relatif datar. Penelitian ini bertujuan untuk mengetahui pengaruh dari pola dan jumlah GCP terhadap akurasi peta orthofoto yang dihasilkan dengan kondisi objek jalan yang berkarakter curam dengan tikungan tajam. Digunakan 4 skenario pola dan jumlah GCP pada area jalan sepanjang ± 500 meter, 4 skenario divalidasi menggunakan RMSEr untuk ketelitian posisi horizontalnya dan RMSEz untuk ketelitian posisi vertikalnya. Dari skenario percobaan yang dilakukan didapatkan hasil yaitu pada skenario pertama dengan GCP 3 buah menghasilkan nilai RMSEr = 0,193 m dan RMSEz = 0,262 m, kedua dengan GCP 4 buah menghasilkan nilai RMSEr = 0,200 m dan RMSEz = 0,608 m, ketiga dengan GCP 4 buah yang diletakan pada setiap tikungan menghasilkan nilai RMSEr = 0,164 m dan RMSEz = 0,429 m, keempat dengan GCP 5 buah menghasilkan nilai RMSEr = 0,134 m dan RMSEz = 0,401 m. Ketelitian geometri  dari empat skenario peta orthofoto yang dihasikan mampu mencapai skala peta 1:2500 dengan interval kontur 1,25 m berdasarkan pedoman teknis ketelitian peta dasar.
The IMPLEMENTASI SISTEM BOOST CONVERTER DAN PENGUKURAN PADA ALAT PENGUSIR HAMA BERBASIS ARDUINO DAN LABVIEW
Authors: Iman Muhammad Rifci Imanullah
Published: September 2023

Penelitian ini penulis melihat permasalahan pada alat pengusir hama yang menghasilkan suara gelombang ultrasonic di atas 20Khz yang hanya dapat didengar oleh kelelawar, paus, dan tikus. baterai yang di charge oleh panel surya. Namun, tegangan baterai akan menurun seiring penggunaan daya dari aki yang berkurang karena pemakaian. Pada penelitian ini dipilih rangkaian boost converter untuk menaikkan baterai sehingga stabil di 13V. Kontrol boost converter menggunakan Arduino dengan keluaran Pulse Width Modulation (PWM) untuk memicu MOSFET. Hasil pengukuran nilai dan grafik data tegangan dan arus akan ditampilkan pada software LabView agar dapat memudahkan dalam pembacaan data.
PENGARUH SUBSTITUSI CANGKANG KERANG DARI ENDE NTT TERHADAP KUAT TEKAN DANKUAT LENTUR BETON fc’= 19,3 MPa
Authors: Ningrum Diana , Setya Handika , Seda Victoria
Published: September 2023

The use of concrete for basic construction materials today is indeed increasing rapidly along with the development of construction construction.Only a concrete quality of 19.3 MPa (fc '= 25 MPa) was considered in this study. Since cement, aggregates, sand and water are extracted from nature, they must be limited. So in this study using additional material clam shells. One of them is shells, shellfish waste or shells are usually only used as decorations or toys so that there is still a lot of waste wasted. Inside the shell, the clam contains pozzolan chemical compounds, namely lime (CaO), aluminum oxide and silica. It can be expected to improve the quality of concrete.

As a result of compressive strength test by changing the clamshell to 0%, 10%, 20%, 30%, 40% and 50%, the average compressive strength value of 0% (ordinary concrete) was 15.81 MPa. The addition of a 10% clam shell results in an average compressive strength value of 20.58 MPa. For the addition of clam shells by 20% obtained an average compressive strength value of 23.37 MPa. 30% yields an average compressive strength of 40.31 Mpa, to 40% of 29.12 Mpa. As for the addition of 50% clam shells, the average compressive strength value is 24.79 Mpa. Thus it can be concluded that the addition of clam shells 30% compressive strength value is higher than the compressive strength value of concrete with the addition of clam shells 0%, 10%, 20%, 40%, and 50%The flexural strength test value in terms of the maximum average moment on the beam for each variation of the test specimen is maximum moment value 75.02 kN.m for 0% variation, maximum moment value 62.52 kN.m for 10% variation, maximum moment value 68.77 kN.m for 20% variation, maximum moment value for 30% variation 62.52 kN.m, 40% variation at maximum moment value of 62.52 kN.m. Therefore, the percentage of specimens that can withstand the highest load in the flexural strength test is 0% and the maximum moment value is 75.02 kN.m.
Eksplorasi Etnomatematika Pada Permainan Tradisional Dedo Gomo dan Gomo Telu di Wolowuwu, Kab. Ende, NTT Serta Integrasinya dalam Pembelajaran Matematika
Authors: Wangge Magdalena
Published: September 2023

Traditional games are one of the cultures that began to fade along with the development of science and technology. This also happens to the people in Wolowuwu, Wolowaru sub-district, Ende district, NTT, namely the dedo gomo and gomo telu games. The purpose of this research is to identify ethnomathematics and mathematical concepts in dedo gomo and gomo telu games and their integration in mathematics learning. This type of research is qualitative research with research stages ranging from pre-field, field work to data analysis. In the data analysis stage, data reduction, data display, and conclusion drawing are carried out. The results obtained are that in the dedo gomo and gomo telu games, ethnomathematics activities are found, namely counting, locating, measuring, designing, playing, and explaining. Then the mathematical concepts found include integer operations, geometry, length measurement, arithmetic series, number patterns, and opportunities. One form of integration in mathematics learning is to prepare learning tools based on traditional game culture with contextual learning models.