bluuebunny/crossref_metadata_2025 huggingface dataset:
MODERN APPEARANCE OF REZEKNE’S ANCIENT LATGALIAN CENTRE
Authors: Ozola Silvija
Published: April 2018

Due to interaction of nature and human activities in regional cities of Latvia a qualitative architectonic space was created over the centuries, which manifests the local inhabitants’ attitude towards art and architecture, and which during the long development process has obtained a characteristic language – a system of relations between well–known signs and symbols for reflection of the deepest nature of creative expression. Looking for harmony in the interaction of the new and historical building through movement, development and contradictions, the dialectics of the architectonic forms is clearly shown. In Latgale the harmony of nature and building encourages you to look for artistically innovative solutions for enrichment of cultural environment. In the centre of Rēzekne beside the Latgalian hillfort, the building complex of the Eastern Latvian Creative Service Centre “Zeimuļs” was built. Roof forms of the lower construction volume are adapted to the hillfort’s relief, but its buildings contrast with surrounding houses, which remind you of sculptures, whose silhouettes are legible on the background of the landscape and sky. The plastic construction of the construction site is clearly revealed in the playful lights of the spatial structure, heterogeneity and dynamics, providing a semantic message to all of us.Topicality of the theme: cities in the Baltic lands developed not only as centres, where values of culture and art were collated, which describe the human being’s higher ideals and confirm the former generations’ achievements in the environment creation, but also their planning and architecture include information on lots of important processes in the past. In the market economy conditions territories of urban historical building are exposed to the impact of development suggestions and new buildings do not always integrate successfully in the architectonically spatial environment created through lots of centuries. Insufficient awareness of culture–historical values affects the quality requirements, thus the research on ancient urban building and planning, preservation of values, also promotion of best practices becomes topical. Goal of the paper: observing objects in nature, applying photo fixations and graphical materials, analyse the correlation of Rēzekne’s ancient and modern architecture, impact of solutions of the earlier ages on the modern urban planning and architectural development.Theoretical research methods: the comparative method is the main one in the research, which gives an opportunity to obtain general and particular conclusions. The logical method or analogous conclusion has also been applied.
IDENTITY OF LATVIAN AND LITHUANIAN CITIES ON THE BALTIC SEASHORE
Authors: Ozola Silvija
Published: April 2018

The Baltic seashore cities Liepaja, Ventspils and Palanga people’s relationships with the sea had an impact on architectural quality of surrounding, which reflect understanding of the economic, culture and art. One of the sustainable development preconditions is identity, which can be achieved by identification, preservation and restoration of cultural heritage, natural objects and specific landscape. Identity of city environment is formed by cultural heritage that encodes information about many processes in the past. There is limited knowledge of the identity of coastal cities in the Baltic countries, where living environment is rich with forms and structures, which can satisfy individual's physical and mental needs, and inspire new ideas. To realize sustainable and balanced development of the Baltic seashore cities Liepaja, Ventspils and Palanga, structural changes are carried out using different spatial development models.The goal of this research is to compare models of sustainable spatial development of the Baltic seashore Latvian cities Liepaja and Ventspils, as well as Lithuanian city Palanga, and to assess their effects on the citizen quality of life.
UNIFORM DESIGN SOLUTION IN EDUCATIONAL INSTITUTION CORPORATE STYLE
Authors: Lesničija Inga , Mežinska Silvija
Published: April 2018

Uniform performs informative and aesthetic function, indicates the user's belonging to a certain institution or organization. This is a business card of sorts, which indirectly informs about the enterprise regardless of its working area. Purpose of the research – to develop a uniform design solution for the employees of the Rezekne Academy of Technologies, based on the study of corporate style features of uniforms, and analogue and textile material research. The research conducted shows the topicality of a uniform with corporate style elements. Analogue assessment justifies Rezekne Academy of Technology employee uniform design solution, based on RTA style book corporate style elements – logo, color palette, and uniform requirements. Analysis of the textile characteristics determines the selection of material and fitting.The research has practical meaning, because it is based on current situation research, user survey and uniform design development. Research base: 37 respondents of Rezekne Academy of Technologies; period of the research 2016–2017.
DEVELOPMENT OF ARCHIVE SYSTEM IN BULGARIA
Authors: Burceva Rita
Published: April 2018

Both Latvian and foreign researchers emphasise the importance of memory institutions (libraries, archives, museums, etc.) in the development of the world view of the public and the basic understanding of cultural values, and a document, which is a primary information carrier in the time and space, is a significant element of culture. The different history of development of various states determined the different peculiarities of the archive systems and their functions in the past and up to nowadays. Studying the specifics of development of archive systems in other countries gives us basis for gaining new experiences and developing cooperation, adopting the positive experience and seeking opportunities to provide access to the documentary heritage for the public needs. The purpose of the research – to study and describe the historical context for the development of the Bulgarian state archives. The object of the research – the peculiarities of development of activity of the state archives of the Republic of Bulgaria. During the course of research the materials collected by researchers of the history of Bulgaria were summarised, some monographs and research papers in professional publications by the archive science specialists were used. The historical origin of the development of Bulgarian archives is directly linked with the important role of Byzantium during the interregnum between the antique and medieval culture in Europe and Middle East. In 1952 the Central State Archive of History, the Central State Archive of the People’s Republic of Bulgaria and regional state archives in the centres of 12 administrative regions. Following the administrative territorial reform from 1959–1963 there was a total of 12 state regional archives established in Bulgaria. During the period between 1961 and 1992 the regional archives are under the direct supervision of municipalities/ people’s councils. In 1974 the third archive – the Central State Technical Archive was established. It can be concluded that regardless of the fact that in the 20th century the existent principles and methods of activity of archives in Russia were introduced in the archive activity in Eastern Europe and the Balkans, the different historical context of the development of various states determined different public attitude towards the preserved documentary heritage in the country, providing access to it and the assessment of the significance of the information included and facts described in the documents.
Informative value of biometric indices of iris, sclera and cornea in primary open-angle glaucoma diagnosis
Authors: Strakhov Vladimir V. , Alekseev Victor V. , Al-Mrrani Abdulgavi M. , Popova Anastasiya A. , Klimova Olga N.
Published: April 2018

Aim: to study the thickness of cornea, iris and scleral tissue, to determine its asymmetry between fellow eyes in healthy subjects and in patients with primary glaucoma. To determine the relationship between changes in biomechanical properties of the cornea and sclera and iris thickness in healthy subjects and in patients with primary glaucoma.

Materials and methods. 10 patients (20 eyes) with primary glaucoma were examined. The control group consisted of 10 people (20 eyes). In all patients ultrasound biomicroscopy (Humphrey Instruments (USA), Model 840) was performed.

Results and discussion. The article presents a study of the corneoscleral and iris tissue thickness in primary glaucoma, as well as the increase pattern of the revealed asymmetry in corneoscleral and iris tissue thickness from normal state to glaucoma. A positive direct correlation between the indices of cornea, sclera, and iris thickness in the primary glaucoma group and between biometric parameters of sclera and iris and the of corneal hysteresis value in primary open-angle glaucoma.
About a new approach to surgical treatment of corneal endothelial dystrophy
Authors: Astakhov Sergey Yu. , Riks Inna A. , Papanyan Sanasar S. , Novikov Sergey A. , Dzhaliashvili Georgiy Z.
Published: April 2018

Primary endothelial dystrophy of the cornea is a fairly common disease in people older than 50 years. Well-developed methods of conservative treatment, as a rule, do not lead to improvement or stabilization of the functional state of the cornea. The choice of tactics of surgical treatment from the existing variety of techniques is complicated. There are isolated reports of the restoration of corneal transparency after descemet membrane removal. The author's method of endothelial corneal dystrophy treatment addressed in this particular clinical case a combination of isolated descemetorhexis and collagen cross-linking resulted in impressive increase in visual acuity and significant improvement in objective criteria for the morpho-functional state of the cornea.
La influencia de los objetivos de compra en la efectividad de las acciones de trade marketing dentro de los supermercados
Authors: Wakabayashi Muroya Jose L. , Alzamora Ruiz Jessica N. , Guerrero Medina Carlos A.
Published: April 2018

La presente investigación analiza la influencia de los objetivos de compra en la efectividad de las acciones de trade marketing implementadas dentro de los supermercados. Para esto se aplicó una encuesta a 800 clientes de este tipo de canales durante su proceso de compra. Los resultados, basados en pruebas de asociación de variables y diferencia de proporciones, evidencian que los consumidores guiados por objetivos de compra concretos serían más sensibles a las acciones de trade marketing que aquellos guiados por objetivos abstractos. Estos hallazgos contribuyen al replanteamiento de las estrategias de trade marketing en supermercados, sugiriendo una segmentación por objetivos de compra del consumidor, con miras a alcanzar una mayor efectividad en mercados con elevada intensidad competitiva, como el latinoamericano.
Satisfacción laboral: una revisión de la literatura acerca de sus principales determinantes
Authors: Pujol-Cols Lucas J. , Dabos Guillermo E.
Published: April 2018

Aun cuando el estudio de la satisfacción laboral ha concentrado un gran interés científico durante buena parte del último siglo, todavía resulta sorprendente cuan fragmentado se encuentra el campo y cuan limitados han  sido los esfuerzos de integración. Este artículo se propone identificar los principales factores que han sido señalados en la literatura empírica como determinantes de la satisfacción laboral, a partir de una revisión de investigaciones publicadas en revistas científicas de alto impacto durante las últimas dos décadas. Los  resultados revelan una insuficiencia de investigaciones que hayan estudiado de manera simultánea los efectos de determinantes disposicionales y situacionales sobre la satisfacción laboral. Finalmente, se analizan los  principales vacíos existentes en la literatura y se sugieren líneas potenciales de investigación.
Optimización de portafolios de inversión con costos de transacción utilizando un algoritmo genético multiobjetivo: caso aplicado a la Bolsa de Valores de Colombia
Authors: De Greiff Samuel , Rivera Juan Carlos
Published: April 2018

Este trabajo aborda la optimización de portafolios teniendo en cuenta restricciones impuestas por los mercados financieros y condiciones de proyectos con exceso de liquidez, como costos de transacción, presupuesto limitado y horizontes de tiempo cortos. Ante estas condiciones, se ha encontrado que los modelos convencionales pueden generar portafolios ineficientes. Por lo tanto, se formula un modelo matemático y se implementa un algoritmo genético multiobjetivo para hallar portafolios eficientes en la Bolsa de Valores de Colombia, minimizando el riesgo y maximizando la rentabilidad. Adicionalmente, se presentan resultados que permiten comparar los portafolios obtenidos con el modelo propuesto y el modelo de media-varianza, mostrando la importancia de los costos de transacción y el presupuesto en la toma de decisiones de inversión.
Глобальный кризис института рецензирования и сетевая модель его преодоления
Authors: Лазуткин Андрей Петрович
Published: April 2018

По мере развития информационно-сетевых технологий научная общественность всё активнее выражает свою обеспокоенность снижением качества научного рецензирования и резким увеличением, в связи с этим, количества фиктивных научных статей. Главная опасность кризиса института рецензирования состоит в том, что поточное производство фиктивных научных статей легитимирует производство столь же фиктивных научно-экспертных рейтингов и статусов. Это, в свою очередь, приводит к системной коррумпированности науки и утрате научным сообществом его важнейшей функции – функции легитимации действий, производимых представителями бизнеса и власти.
Глубочайший системный кризис науки объясняется в статье тем, что современное научное рецензирование подчинено методологически несостоятельным принципам бюрократического дискурса – дискурса без обратной связи, без какой-либо возможности апелляции со стороны автора. Постулируется задача создания действенной альтернативы дискурсу, в котором рецензент, как правило анонимный, выступает по отношению к автору статьи оппонентом и судьёй в одном лице. Технологической основой такой альтернативы обозначена самоорганизуемая система модерации сетевых экспертных дискуссий (онлайн-дебатов), описание которой представлено в статье.
Технология «Онлайн-дебаты», общее описание которой приведено в статье, предусматривает равноправное ролевое взаимодействие рецензента, автора и редактора и представляет собой сетевой аналог судебного состязания прокурора, адвоката и судьи. Уникальной особенностью описываемой технологии является механизм определения объективного рейтинга суждений и личного репутационного рейтинга участников публичного (сетевого) рецензирования.
Asociación entre factores socioambientales y la práctica de actividad física en mujeres embarazadas: estudio de corte transversal
Authors: De Avila-Quintana Luzdaris , Ariza-Egea Sindy , Llanos-González Boris , Herazo-Beltrán Yaneth , Domínguez-Anaya Regina
Published: April 2018

Objetivo: establecer la asociación entre factores socioambientales y la práctica de actividad física en mujeres embarazadas.Materiales y métodos: estudio de corte transversal realizado en mujeres gestantes sin contraindicaciones para realizar actividad física, que asistían a la consulta prenatal, pertenecientes a dos instituciones de salud de la ciudad de Barranquilla y otra de la ciudad de Cartagena de Indias, Colombia. El muestreo fue intencional. Se evaluaron las características sociodemográficas, los niveles de actividad física y la percepción de factores socioambientales como infraestructura del barrio, las cualidades estéticas, el ambiente social, la conexión entre las calles, la seguridad y tráfico en el barrio. Se estimó la razón de odds (OR).Resultados: de un total de 228 mujeres gestantes se incluyeron 208 en el análisis. El 62% de las gestantes no cumplen las recomendaciones de actividad física para mantener su salud. Entre los factores socioambientales relacionados con la inactividad física de las embarazadas están el residir a mucha distancia de tiendas y supermercados para ir caminando (OR = 5,1; IC 95 %: 2,8-9,4); presencia de aceras en mal estado en las calles (OR = 3,4; IC 95 %: 1,9-6,3); pocas personas físicamente activas en el barrio (OR = 4,1; IC 95 %: 2,2-7,5); inseguridad en el día y en la noche para caminar (OR = 3,6; IC 95 %: 1,9-6,4).Conclusión: la percepción de las características socioambientales está asociada a la actividad física de las gestantes. Se requieren intervenciones que mejoren las características socioambientales que afectan la percepción de estos factores en la población, para evaluar su efecto en la actividad física de las gestantes.
El aborto: una mirada desde la salud pública, los derechos y la justicia social
Authors: Gómez-Dávila Joaquín Guillermo
Published: April 2018

el aborto inseguro se considera un problema de salud pública (SP), de derechos y de justicia social (JS). Esto es particularmente certero para las mujeres que viven en países en vías de desarrollo, por lo que se requiere de una SP que defienda la dignidad y que fomente el ejercicio de los derechos y generen las condiciones necesarias para que las mujeres sean dueñas de su autonomía reproductiva. El articulo analiza tres corrientes de justicia social: el utilitarismo, el liberalismo y el desarrollo de capacidades y analiza sus fortalezas y contradicciones. Concluye que el desarrollo de capacidades y la JS que de ella se deriva sería ideal para una SP que pretende el mayor grado de salud y bienestar posibles, aseguraría la satisfacción de todas las condiciones materiales básicas para el florecimiento de las capacidades y por ende aseguraría una mayor participación en la construcción de vidas propias; tendría presente la realidad que viven las personas en sus entornos socioculturales, y posibilitaría sacar del espacio privado el mundo femenino y permitir el debate público sobre dichas cuestiones e impedir que sigan siendo consideradas como cuestiones “naturales” e “inmutables de las relaciones humanas”, lo que garantizaría una mayor pertinencia en la satisfacción de las necesidades de cada población. También recalca que la JS que caracteriza a este enfoque no llegará de arriba, del Estado, sino que requiere de una construcción colectiva, donde los movimientos contra hegemónicos juegan un papel muy importante y hacen parte de la propia construcción de las capacidades.
Opilación y supresión de menstruos: una receta médica del siglo XVIII en el Nuevo Reino de Granada
Authors: Vásquez Carvajal Carolina , Uribe-Vergara Jorge , Martínez-Lozano Julio César , Gómez-Gutiérrez Alberto , Briceño-Balcázar Ignacio
Published: April 2018

Objetivo: Presentar y definir el contexto de una receta médica que data del siglo XVIII en el Nuevo Reino de Granada, utilizada para la supresión de la menstruación, así como analizar a la luz de los conocimientos actuales si sus ingredientes en conjunto podrían o no lograr la inhibición del sangrado menstrual.Metodología: Se realizó una búsqueda documental en el archivo histórico de la biblioteca Octavio Arizmendi Posada en la Universidad de la Sabana en la que se encuentra la receta “Opilacion y supresión de menstruos”, y posteriormente se condujo una revisión de la literatura disponible en las bases de datos SciELO/proQuest en el periodo 1993 - 2015.  Conclusiones: En la receta manuscrita hallada en el archivo histórico de la Universidad de la Sabana se describen múltiples ingredientes que, analizados a la luz de los conocimientos actuales, y en su conjunto, no tienen validez para lograr el pretendido efecto supresor. Sin embargo, se reconocen las propiedades antianémicas del “vitriolo líquido de Marte” y el posible efecto que ejercería el ejercicio excesivo formulado en medio del ciclo menstrual, aunque en la receta no se describe con exactitud la frecuencia ni la intensidad con la que este debería ser realizado para lograr una supresión del menstruo.
A Mixed-Methods Approach to Analyze Shared Epistemic Agency in Jigsaw Instruction at Multiple Scales of Temporality
Authors: Oshima Jun , Oshima Ritsuko , Fujita Wataru
Published: April 2018

The purpose of this study was to propose a mixed-methods approach to analyzing shared epistemic agency in jigsaw instruction from multiple temporal perspectives, and to evaluate its effectiveness by examining actual datasets. We employed a combination of socio-semantic network analysis (SSNA) and in-depth dialogical discourse analysis as a mixed-methods approach, and analyzed discourse transcripts by university students engaged in jigsaw instruction. First, we graphically depicted a quantitative measure of shared epistemic agency at the group level and identified pivotal points of discourse where students might engage in an epistemic action toward alleviating a lack of knowledge. Then, we conducted dialogical discourse analysis of the segments around the pivotal points to describe students' collaboration practices. SSNA represented the quantitative nature of shared epistemic agency with 60% accuracy and provided a new way to look at it as a distribution of pivotal points for alleviating a lack of knowledge across all processes of jigsaw group activities. The dialogical discourse analysis of the discourse segments identified by SSNA further described dialogical patterns in the shared epistemic agency and each student's contribution to them..
A Novel Method for the In-Depth Multimodal Analysis of Student Learning Trajectories in Intelligent Tutoring Systems
Authors: Liu Ran , Stamper John C , Davenport Jodi
Published: April 2018

Temporal analyses are critical to understanding learning processes, yet understudied in education research. Data from different sources are often collected at different grain sizes, which are difficult to integrate. Making sense of data at many levels of analysis, including the most detailed levels, is highly time-consuming. In this paper, we describe a generalizable approach for more efficient yet rich sensemaking of temporal data during student use of intelligent tutoring systems. This multi-step approach involves using coarse-grain temporality — learning trajectories across knowledge components — to identify and further explore “focal” moments worthy of more fine-grain, context-rich analysis. We discuss the application of this approach to data collected from a classroom study in which students engaged in a Chemistry Virtual Lab tutoring system. We show that the application of this multi-step approach efficiently led to interpretable and actionable insights while making use of the richness of the available data. This method is generalizable to many types of datasets and can help handle large volumes of rich data at multiple levels of granularity. We argue that it can be a valuable approach to tackling some of the most prohibitive methodological challenges involved in temporal learning analytics
Preoperative hemogram-related parameters to distinguish renal cell carcinoma from benign kidney masses: HERR score
Authors: TÜRKOĞLU Ali Rıza , ÜSTÜNDAĞ Yasemin
Published: April 2018

Objectives: Renal cell carcinoma (RCC) accounts for approximately 90% of all kidney malignancies, and it is difficult to preoperatively distinguish between tumors and benign masses without a kidney biopsy in small renal masses. We investigated whether any preoperatively defined hemogram-related parameters had a predictive value that would distinguish RCC from benign kidney masses using a novel scoring method. Methods: Between January 2011 and November 2017, 330 patients diagnosed with kidney masses and who received an operation were included. Fifty-six masses were benign. The neutrophil-to-lymphocyte count (NLR), platelet-to-lymphocyte count, lymphocyte-to-monocyte count, mean platelet volume, platelet count ratio, and hemoglobin to red cell distribution width ratios were calculated. The hemogram-related parameters were combined with the tumor size to establish the hemogram-related risk (HERR) score. The area under the receiver operating characteristics curve, sensitivity, specificity, and likelihood ratios were evaluated to preoperatively diagnose RCC.Results: Histological findings confirmed RCC in 274 patients. The NLR [median (interquartile range)] was higher in patients with RCC, 3.7 (4.7), compared to a benign kidney mass, 2.4 (2.2) (p < 0.001). A HERR score cut-off of ≥ 3 showed a good sensitivity at 78% with an LR+ of 10.8 [95% confidence interval (CI): 7.0-16.4] and an LR- of 1.2 (95% CI: 1.0-1.5). Conclusion: Our study, despite being a preliminary validation, is the first to evaluate hemogram-related parameters for preoperatively discriminating between RCC and benign renal masses, and the HERR score serves as a potential diagnostic biomarker for this.
Differential proteome analysis of pea roots at the early stages of symbiosis with nodule bacteria
Authors: Leppyanen I. V. , Kirienko A. N. , Lobov A. A. , Dolgikh E. A.
Published: April 2018

In this paper, we have analyzed changes in the proteomic spectrum of pea Pisum sativum L. roots during inoculation with rhizobial bacteria with the aim of revealing new regulators of symbiosis development. To study the changes in the proteome spectrum of pea roots, a differential twodimensional (2-D) electrophoresis was performed using fluorescent labels Cy2 and Cy5. The images obtained made it possible to identify differences between the control variant (uninoculated roots) and the root variant after inoculation with Rhizobium leguminosarum bv. viciae RCAM 1026 (24 hours after treatment). 20 proteins were revealed and identified, the synthesis of which was enhanced during the inoculation of pea roots by nodule bacteria. To identify the proteins, a mass spectrometric analysis of tryptic peptides was performed on a quadrupole-time-of-flight mass spectrometer combined with a high-performance liquid chromatograph. Among such proteins, the beta-subunit of the G protein and the disulfide isomerase/phospholipase C were first found, whose function can be related to the signal regulation of symbiosis. This indicates that G-proteins and phospholipases can play a key role in the development of early stages of symbiosis in peas. Further experiments are expected to show whether the beta-subunit of the G protein interacts with the receptors to Nod factors, and how this affects the further signaling. Other proteins that might be interesting were annexin D8 and D1, protein kinase interacting with calcinerin B, actin-binding protein profilin, GTP-binding protein Ran1. They may be involved in the regulation of reactions with calcium, the reorganization of the actin cytoskeleton and other important processes in plants. The study of the role of such regulatory proteins will later become the basis for understanding the complex system of signal regulation, which is activated in pea plants by interaction with nodule bacteria.
Genetic polymorphism of CYP1A1 and CYP2D6 in populations of Buryats, Teleuts and Russians of Eastern Siberia
Authors: Tabikhanova L. E. , Osipova L. P. , Churkina T. V. , Voronina E. N. , Filipenko M. L.
Published: April 2018

The study of the gene polymorphism of the system of biotransformation of xenobiotics is an important area of modern medical and genetic research. The aim of this work is to study the frequency of the alleles of the CYP1A1 (A2455G (*2C), rs1048943), CYP2D6 (A2549del (*3), rs35742686); G1846A (*4), rs3892097) genes of Teleuts (n = 115), Eastern Buryats (n = 132), Western Buryats (n = 280), their Métis (n = 56), and Russians of East Siberia (n = 122). Genotyping was performed using real-time PCR with competitive TaqMan allele-specific probes. The frequency of the CYP1A1*2C (2455G) allele was 28.8 % in the Eastern Buryat, 34.6 % in the Western Buryat, 16.7 % in the Teleut, and 31.3 % in the Métis cohort. The frequency of CYP1A1*2C (2455G) in the Russians of Eastern Siberia (4.1 %) corresponds to the frequency range found in European populations. A high-frequency occurrence of CYP1A1*2C (2455G) among Buryats and Teleuts may be indicative of a higher population-wide risk of diseases influenced by technogenic pollutants – substrates of CYP1A1. The CYP2D6*3 (2549del) allele was not detected in cohorts of indigenous populations, among Russians it was 0.4 %, and it was 2.7 % among Métis. The frequency of CYP2D6*4 (1846A) in Eastern and Western Buryats was 5.3 % and 4.3 %, respectively, for Teleuts it was 7.4 %. It was significantly higher in the Russian population (12 %), and among Métis (9.8 %). The obtained data makes it possible to predict a reduced risk of side effects of drugs and cancer associated with CYP2D6*3 (2549del) and CYP2D6*4 (1846A) in the Buryat and Teleut populations. However, metisation introduces new polymorphic variants into indigenous populations, shifts gene frequencies and changes the degree of risks.
Tissue-specific effects of benzo[a]pyrene and DDT on microRNA expression profile in female rats
Authors: Ushakov D. S. , Kalinina T. S. , Dorozhkova A. S. , Ovchinnikov V. Y. , Gulyaeva L. F.
Published: April 2018

Many xenobiotics in the human environment, such as benzo[a]pyrene (B(a)P) and dichlorodiphenyltrichloroethane (DDT), may act as non-genotoxic carcinogens through epigenetic mechanisms, including changes in microRNA expression profile. In part, such disorders can be mediated by the activation of nuclear receptors, resulting in the activation of protein coding gene expression and microRNAs involved in malignant transformation of cells. Therefore, the aim of this study was to investigate the chain of events “xenobiotic administration – receptor activation – up-regulating microRNA expression – down-regulation target genes expression” as one of the key factors in the chemically-induced carcinogenesis. Using in silico methods, an analysis of the rat genome was carried out to find microRNAs putatively regulated by AhR (aryl hydrocarbon receptor) and CAR (constitutive androstane receptor), activated by BP and DDT, respectively. In particular, miR-3577 and -193b were selected as potentially regulated CAR, miR-207 was selected as a candidate for miR under AhR regulation. The results of the study showed that the treatment of female rats with DDT and B(a)P caused a tissue-specific changes in the expression of microRNAs and host genes in both acute and chronic administration of xenobiotics. To confirm the effects of xenobiotics on the microRNA expression, we also estimated the mRNA level of PTPN6, EIF3F, Cbx7, and Dicer1 genes potentially targeting miR-193b, -207, and -3577. The study has shown a high correlation between the expression of target genes and microRNAs; however these changes depended on the tissue types, the dose and time after xenobiotic treatment.
Changing of expression actinbinding proteins in the kidney under dehydration
Authors: Khegay I. I.
Published: April 2018

Actin is related to main structural proteins in eukaryotes. In opposite to muscle alpha-actin, beta-actin is expressing in all types of cells. The constant reorganization of actin cytoskeleton takes place in non-muscle cells. Fibrillar actin, organized by globular monomers, interacts with the actin-binding proteins. Alpha-actinin forms a transverse links in actin fibrillar network, as well as concentrates in the fields of focal contacts. Tropomyosin is related to regulatory components of beta-actin, and due to the expense of longitudinal localization of the molecule in the groove of actin microfilament, stereochemically shields the sites of other actin-binding proteins. The most important function of actin cytoskeleton is the participation in the transportation of vesicles with aquaporins of second type in principal cells of an epithelium of collecting ducts in renal medulla. Vasopressin is stimulating the release of aquaporin tetramers from cytoplasmic store to apical plasmatic membrane. The participation and role of separate cytoskeleton proteins in the process of aquaporin trafficking and forming additional pores for water stays a poorly studied place in the molecular physiology of kidney. We explored the osmoregulatory action of prolonged hydration and dehydration on the protein composition of actin cytoskeleton in rats depending on the presence or absence of the actively expressing vasopressin gene in the genome. We found that the efficiency of the renal concentrating system, controlled by vasopressin, depends on expression of actin-binding proteins in the renal medulla. On the background of a stable level of inner cellular beta-actin, a change of expression an alpha-actinin and tropomyosin is observed. Dehydration of the organism is accompanied by essential reducing of alpha-actinin. In the absence of vasopressin, reduction of alpha-actinin has a smaller amplitude. The presence of the normal vasopressin gene in the genome, regardless of transitory expression level and secretion of hormone, is a factor of lower tropomyosin in the kidney. The most probable molecular mechanism of changing the expression of the genes for alpha-actinin and tropomyosin is transduction of the V2-mediated vasopressin hormonal signal to protein kinase A, phosphorylation of the cAMPresponsible transcriptional factor CREB, and nuclear CREB interaction with gene CRE sites sensitive to it.
Homologs of the rolC gene of naturally transgenic toadflaxes Linaria vulgaris and Linaria creticola are expressed in vitro
Authors: Matveeva T. V. , Bogomaz O. D. , Golovanova L. A. , Li Yu. S. , Dimitrov D.
Published: April 2018

Agrobacterium mediated transformation is the most common way for obtaining transgenic plants in laboratory conditions. At the same time, there are species inside the genera Nicotiana, Linaria and Ipomoea that contain homologs of agrobacterial T-DNA genes as a result of genetic transformation of their ancestral forms in natural conditions. Such plants are called naturally transgenic plants, and T-DNA in their genomes is called cellular (cT-DNA). It is proposed that in the evolution of these genera, the introduced sequences played an important role. This idea is confirmed by the data on the expression of some T-DNA genes in Nicotiana and Ipomoea. Until the last moment, the expression of cT-DNA genes in Linaria has not been documented. However, the analysis of the nucleotide sequence indicates the functionality of rolC gene in L. vulgaris Mill., L. acutiloba Fisch. ex Rchb., L. genistifolia (L.) Mill. In this research work, we have sequenced the rolC homolog in one more toadflax species (Linaria creticola Kuprian). The in silico analysis of this gene has shown that it can encode a full-length peptide. Using the real time RT-PCR method, we have demonstrated that the rolC homolog is expressed in vitro in shoots, roots and calli of L. vulgaris Mill., as well as in shoots of L. creticola Kuprian. The results obtained are an important argument in favor of the fact that cT-DNA is functional and that its fixation in genomes played a certain role in the evolutionary process. However, the level of expression of the gene studied is quite low. A similar trend was observed in other naturally transgenic species. This can explain the absence of explicit morphological differences of species containing cT-DNA from their non-transgenic relatives.
Information system on microbial collections as a part of bioresource collections portal for Russia’s FASO organizations: a working protocol
Authors: Kazantsev F. V. , Smirnova A. A. , Rozanov A. S. , Uvarova Yu. E. , Afonnikov D. A. , Peltek S. E. , Lashin S. A.
Published: April 2018

Nowadays, many scientific organizations of Russia own collections of microorganisms on which large volumes of information have been generated. These data represent the descriptions of objects of diverse nature (bacteria, archaea, fungi, protists) and their properties, which have been carefully collected and cataloged by generations of researchers. Not every organization that has such collections has an open access electronic catalog, which not only complicates work with these unique materials, but also even hides the fact of the existence of such collections. This state of affairs requires the development of electronic resources for presenting these materials to the scientific community. To put together the information on microorganism collections, we have developed an internet portal (http://www.biores.cytogen.ru/microbes/) of microbial bioresource collections of FASO organizations in the Russian Federation. The portal was created under the project developing the information system for bioresource collections of FASO institutes. It is a platform where collection organizations can place information about the storage units of their collections, as well as other information on collections, including links to their own catalogs. In this paper, we describe the principles of working with the portal. The portal’s graphical interface allows users, both registered and unregistered, to receive the following information about collections of microorganisms: a list of collections represented in the database, contact details of the organization and information about the curator of the collection, summary statistics for each collection, as well as information on storage units. Registered users – owners of collections – have the opportunity to create and modify records about the storage units of their collections, and to update their description. To automate work with the portal, software access to the database through the REST API has been implemented (http://api.biores.cytogen.ru/ microbes/). At present, the portal is still being filled, but it already contains a description of more than 13,000 items of storage (of which 3500 are in the microorganisms’ part) of 65 bioresource collections in Russia’s FASO organizations. Of these collections, 12 with microorganisms have a total diversity of funds of about 50,000 strains).
Desafíos para pensar… la enseñanza de la filosofía
Authors: Kohan Walter Omar
Published: April 2018

Los problemas que plantea la enseñanza de la filosofía son múltiples, pues supone lo que entendemos por filosofía y por enseñar y aprender filosofía. En este sentido la enseñanza y el aprendizaje se convierten en un problema filosófico, en un reto que da que pensar, tal como ya lo habían entrevisto Sócrates y Platón. El  problema se torna aún más complejo cuando nos preguntamos por la significación de enseñar filosofía ante las dificultades y situaciones de los pueblos latinoamer
Bulut Bilişim Teknolojileri Kabul Modeli 3: Ölçek Uyarlama Çalışması
Authors: Hamutoğlu Nazire Burçin
Published: April 2018

Bu çalışmada Venkatesh ve Bala tarafından 2008 yılında geliştirilen 51 maddeden oluşan “Teknoloji Kabul Modeli 3 Ölçeğinin” Türkçe ’ye uyarlaması yapılmıştır. Ölçeğin orijinali “algılanan fayda, algılanan kullanım kolaylığı, bilgisayar öz-yeterliği, dışsal kontrol algısı, bilgisayar eğlenceliği, bilgisayar kaygısı, algılanan keyif, sübjektif norm, gönüllülük, imaj, iş ile ilgililik, çıktı kalitesi, sonuçların gösterilebilirliği, davranışsal niyet, kullanım ve nesnel kullanılabilirlik” olmak üzere 16 alt boyuttan oluşmaktadır.  Ölçeğin Türkçe’ ye uyarlama çalışmaları dilsel eşdeğerlik ile başlamış, ardından AFA, DFA ve güvenirlik analizleri ile devam etmiştir. AFA için 356 öğrenciden elde edilen veriler, DFA için ise farklı 436 öğrenciden elde edilen veriler kullanılmıştır. Ayrıca iç tutarlılık (Cronbach Alpha) ve kompozit güvenirlik katsayıları ile de ölçeğin güvenirliğine bakılmıştır.  Yapılan analizler sonucunda ölçeğin Türkçe formunun geçerli ve güvenilir bir yapıya sahip olduğu görülmüştür.
Türkçe Öğretim Sürecinde Dijital Bölünme
Authors: BAL Mazhar , USLU Esra
Published: April 2018

Bu çalışmanın amacı, Türkçe öğretim sürecinde öğretmenlerle öğrenciler arasındaki dijital bölünmeyi ele almak ve dijital bölünmenin Türkçe öğretim sürecine etkisini ortaya koymaktır. Araştırma, nitel araştırma yöntemlerinden örnek olay incelemesi ile desenlenmiştir. Kolay ulaşılabilir durum örneklemesi ile seçilen araştırmanın katılımcılarını, iki farklı grup oluşturmaktadır: (1) Türkçe öğretim sürecini teknolojiyle ilişkilendirme konusunda becerisi yüksek öğretmenler ve onların teknoloji kullanımı konusunda becerisi düşük öğrencileri; (2) Türkçe öğretim sürecini teknolojiyle ilişkilendirme konusunda becerisi düşük öğretmenler ve onların teknoloji kullanımı konusunda becerisi yüksek öğrencileridir. Veri toplama aracı olarak yarı yapılandırılmış görüşme formu, anket kullanılmıştır. Toplanan veriler, nitel veri analiz programı kullanılarak çözümlenmiştir. Araştırmada Türkçe öğretmenleri ve öğrencileri arasında farkındalık, beceri ve isteklilik açısından dijital bölünme olduğu sonucuna ulaşılmıştır. Öğretim sürecinde dijital bölünmenin olumsuz etkilerini engellemek için öğretmen ve öğrencilerin teknoloji kullanımı konusunda becerileri, okul içi ve okul dışı teknolojik imkanların göz önünde bulundurulması gerektiği düşünülmektedir.
The Effect of Slimming Herbs Tea With Dietary Adjustment To The Result Of Antropometric Measurement Among Women Member of Aerobic Studio
Authors: Perslami Resti , Puruhito Edith Frederika , Agil Mangestuti
Published: April 2018

Background: Overweight is the accumulation of excessive fat that can harm health. People who are overweight usually have a size of body anthropometry more than normal, such as the size of the waist circumference (WC), upper arm circumference (UAC), and subcutaneous fat thickness (SFT). Women are more to being overweight, because metabolism in women is lower and body fat is more than men. They attempt to return to normal body weight, by following the fitness clubs, diet and so forth. In this study, researchers used a slimming herbs tea to help restore their body size. Purpose : This study was to determine the effects of slimming herbs tea with dietary adjustments to the results of anthropometric measurements in the group of gymnastic class women. Methods: The method used is quasy experimental design with the design of "pre-posttest control group design”. The study was held for 14 days by the number of respondents 14 people. Respondents were divided into 2 groups: control and treatment (consumption of slimming herbs tea). Slimming herbs tea composition consisting of green tea, guazuma extracts, parameria extract, fennel extract and curcuma extract consumed 2 times a day after meals every day. Data were analyzed using SPSS with Paired T-Test. Results: The results showed slimming herbs tea with dietary adjustments can reduce the results of anthropometric measurements such as WC as much as 6,21 cm ,UAC as much as 2,2 cm ,and SFT as much as 2,7 mm. While the reducing measurement in control group, in WC as much as 2,57 cm, UAC as much as 1,6 cm, SFT as much as 1,4 mm. Conclusion: The effect of slimming herbs tea with dietary adjustment can reducing the result of anthropometric measurement more in the woman class of gymnastic
State Anxiety Inventory Score Among Students With Final Assignment
Authors: Imandiri Ario , Widyastuti Erlinda , Jonosewojo Arijanto
Published: April 2018

Background: Students final assignment is a scientific work requires precision, time consuming, energy as well as concentrations. Students may experience stress within the process of completing the final assignment, which can lead to variety of disorder; physical, emotional, intellectual and interpersonal. Purpose: The aim of this study was to determine the state anxiety inventory scores among final students with scientific project. Those score were then being used as preliminary data to decide the next step of the study which are giving acupuncture therapies to those students with high score of anxiety.  Methods: This was a descriptive study with one shot/cross sectional study, using questionnaires distributed to respondents for data completion. Respondents were 100 students from Faculty of Vocational Education Universitas Airlangga who were taking the final assignment subject. Results: The results of State Anxiety Inventory Score were obtained as average score of 53.03 with standard deviation of 10.4. Conclusion: The mean values showed a reasonably anxious outcome.
Fabrication Technique of Dental Restoration Using Hybrid Ceramic With Cad Cam Method
Authors: Andriyani Meita , Harwasih Sonya , Inayati Eny
Published: April 2018

Background :  Ceramic is superior in aesthetic but fragile and breakable under bite pressure . Lack of understanding of material requirements for resistance in the oral cavity and minimal ceramic processing techniques is the initial cause of the failure. Hybrid ceramic dental restoration is a material that combines the good properties of ceramics and composites that have elasticity and ensures high strength and minimize the wall thickness of the restoration. Mechanical manufacture of dental restorations currently growing, CAD CAM systems are becoming popular in the field of dentistry. CAD CAM provides the advantage that the effectiveness of the time, does not require a lot of human resources, and produce a restoration with good quality. Purpose:  To explain hybrid ceramic material and techniques of making  hybrid ceramic dental restorations with CAD CAM system. Review: Hybrid ceramic is a material that combines the advantages of ceramics and composite elasticity. This material contains a hybrid structure with two networks, ceramic and polymer are linked to each other, known as double hybrid network. It added that the structure of the ceramic feldspathic network (86% wt) is reinforced by a polymer network (14% wt) are integrated as a polymer network filling cavities that exist in the network and make its structure ceramic hybrid ceramic material becomes denser. Conclusion: Hybrid ceramic having chewing load capacity and high elasticity, flexural strength  150-160 Mpa and fracture toughness 1.5 Mpa, higher than conventional ceramics. Mechanical manufacture of dental restorations using ceramic hybrid materials with CAD CAM method begins with scanning, selection of materials and tooth shade, designing, milling, followed by finishing, polishing, ends with staining and glazing.
Results of BACE1-Inhibitor Clinical Trials Confirm Key Predictions For App-Independent Generation of Beta-Amyloid in Sporadic AD
Authors: Volloch Vladimir , Rits Sophia
Published: April 2018

The present article analyzes the results of recent clinical trials of beta secretase inhibition in sporadic Alzheimer’ disease (SAD), considers the striking dichotomy between successes in tests of BACE1 inhibitors in healthy subjects and familial AD (FAD) models versus persistent failures of clinical trials and interprets it as a confirmation of key predictions for a mechanism of APP-independent, beta secretase inhibition-resistant production of beta amyloid in SAD, previously proposed by us.  In the light of this concept, FAD and SAD should be regarded as distinctly different diseases as far as beta-amyloid generation mechanisms are concerned, and whereas beta secretase inhibition would be neither applicable nor effective in treatment of SAD, the BACE1 inhibitor(s) deemed failed in SAD trials could be perfectly suitable for treatment of FAD. Moreover, targeting the aspects of AD other than cleavages of the APP by beta and alpha secretases should have analogous impacts in both FAD and SAD.