bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Aichinger’s equation to characterize polynomial functions
Authors: Almira J. M.
Published: November 2022

Aichinger’s equation is used to give simple proofs of several well-known characterizations of polynomial functions as solutions of certain functional equations. Concretely, we use that Aichinger’s equation characterizes polynomial functions to solve, for arbitrary commutative groups, Ghurye–Olkin’s functional equation, Wilson’s functional equation, the Kakutani–Nagumo–Walsh functional equation, and a general version of Fréchet’s unmixed functional equation.
A note on strong conciseness in virtually nilpotent profinite groups
Authors: Detomi Eloisa
Published: November 2022

A group word w is said to be strongly concise in a class $${\mathcal {C}}$$
C
of profinite groups if, for every group G in $${\mathcal {C}}$$
C
such that w takes less than $$2^{\aleph _0}$$

2

ℵ
0

values in G, the verbal subgroup w(G) is finite. In this paper, we prove that every group word is strongly concise in the class of virtually nilpotent profinite groups.
Mechanisms of colorectal liver metastasis development
Authors: Shasha Tal , Gruijs Mandy , van Egmond Marjolein
Published: November 2022

Colorectal cancer (CRC) is a leading cause of cancer-related death worldwide, largely due to the development of colorectal liver metastases (CRLM). For the establishment of CRLM, CRC cells must remodel their tumor-microenvironment (TME), avoid the immune system, invade the underlying stroma, survive the hostile environment of the circulation, extravasate into the liver, reprogram the hepatic microenvironment into a permissive pre-metastatic niche, and finally, awake from a dormant state to grow out into clinically detectable CRLM. These steps form part of the invasion-metastasis cascade that relies on reciprocal interactions between the tumor and its ever-changing microenvironment. Such interplay provides a strong rational for therapeutically targeting the TME. In fact, several TME constituents, such as VEGF, TGF-β coreceptor endoglin, and CXCR4, are already targeted in clinical trials. It is, however, of utmost importance to fully understand the complex interactions in the invasion-metastasis cascade to identify novel potential therapeutic targets and prevent the establishment of CRLM, which may ultimately greatly improve patient outcome.
Seven classes of antiviral agents
Authors: Ianevski Aleksandr , Ahmad Shahzaib , Anunnitipat Kraipit , Oksenych Valentyn , Zusinaite Eva , Tenson Tanel , Bjørås Magnar , Kainov Denis E.
Published: November 2022

The viral epidemics and pandemics have stimulated the development of known and the discovery of novel antiviral agents. About a hundred mono- and combination antiviral drugs have been already approved, whereas thousands are in development. Here, we briefly reviewed 7 classes of antiviral agents: neutralizing antibodies, neutralizing recombinant soluble human receptors, antiviral CRISPR/Cas systems, interferons, antiviral peptides, antiviral nucleic acid polymers, and antiviral small molecules. Interferons and some small molecules alone or in combinations possess broad-spectrum antiviral activity, which could be beneficial for treatment of emerging and re-emerging viral infections.
Cardiovascular parameters on computed tomography are independently associated with in-hospital complications and outcomes in level-1 trauma patients
Authors: Kobes Tim , Sweet Arthur A. R. , Klip IJsbrand T. , Houwert Roderick M. , Veldhuis Wouter B. , Leenen Luke P. H. , de Jong Pim A. , van Baal Mark C. P. M.
Published: November 2022

Background

In-hospital complications after trauma may result in prolonged stays, higher costs, and adverse functional outcomes. Among reported risk factors for complications are pre-existing cardiopulmonary comorbidities. Objective and quick evaluation of cardiovascular risk would be beneficial for risk assessment in trauma patients. Studies in non-trauma patients suggested an independent association between cardiovascular abnormalities visible on routine computed tomography (CT) imaging and outcomes. However, whether this applies to trauma patients is unknown.

Purpose

To assess the association between cardiopulmonary abnormalities visible on routine CT images and the development of in-hospital complications in patients in a level-1 trauma center.

Methods

All trauma patients aged 16 years or older with CT imaging of the abdomen, thorax, or spine and admitted to the UMC Utrecht in 2017 were included. Patients with an active infection upon admission or severe neurological trauma were excluded. Routine trauma CT images were analyzed for visible abnormalities: pulmonary emphysema, coronary artery calcifications, and abdominal aorta calcification severity. Drug-treated complications were scored. The discharge condition was measured on the Glasgow Outcome Scale.

Results

In total, 433 patients (median age 50 years, 67% male, 89% ASA 1–2) were analyzed. Median Injury Severity Score and Glasgow Coma Scale score were 9 and 15, respectively. Seventy-six patients suffered from at least one complication, mostly pneumonia (n = 39, 9%) or delirium (n = 19, 4%). Left main coronary artery calcification was independently associated with the development of any complication (OR 3.9, 95% CI 1.7–8.9). An increasing number of calcified coronary arteries showed a trend toward an association with complications (p = 0.07) and was significantly associated with an adverse discharge condition (p = 0.02). Pulmonary emphysema and aortic calcifications were not associated with complications.

Conclusion

Coronary artery calcification, visible on routine CT imaging, is independently associated with in-hospital complications and an adverse discharge condition in level-1 trauma patients. The findings of this study may help to identify trauma patients quickly and objectively at risk for complications in an early stage without performing additional diagnostics or interventions.
Parenthood and psychological distress among English Millennials during the second wave of the COVID-19 pandemic: evidence from the Next Steps cohort study
Authors: Chen B. , McMunn A. , Gagné T.
Published: November 2022

Purpose

The COVID-19 pandemic led to disproportionate mental health responses in younger adults and parents. The aim of the study was to investigate how Millennial parents’ experiences were associated with psychological distress over the first year of the pandemic.

Methods

We examined data in September 2020 (n men = 994; n women = 1824) and February 2021 (n men = 1054; n women = 1845) from the Next Steps cohort study (started ages 13–14 in 2003–04). In each wave, we examined differences in GHQ-12 scores between parent groups defined by the age and number of children, adjusting for background characteristics at ages 13–14, psychological distress at ages 25–26, and other circumstances during the pandemic. We also examined if differences varied by work status, financial situation before the outbreak and relationship status.

Results

Whereas mothers with one or two children and children aged 0–2 reported less distress than non-mothers in September 2020, there were no such differences in February 2021. Fathers with three or more children reported more distress in February 2021. Compared with non-fathers who worked, fathers were also disproportionally distressed if they were working with one child or with children aged 2 or less in September 2020.

Conclusion

The distribution of psychological distress among Millennial parents and non-parents has varied by age, sex, parenting stage, work status and the timing of the pandemic. Generous family policies are needed, with special attention dedicated to parents combining work and family responsibilities.
Markov risk mappings and risk-sensitive optimal prediction
Authors: Kosmala Tomasz , Martyr Randall , Moriarty John
Published: November 2022

We formulate a probabilistic Markov property in discrete time under a dynamic risk framework with minimal assumptions. This is useful for recursive solutions to risk-sensitive versions of dynamic optimisation problems such as optimal prediction, where at each stage the recursion depends on the whole future. The property holds for standard measures of risk used in practice, and is formulated in several equivalent versions including a representation via acceptance sets, a strong version, and a dual representation.
Perfluoroalkyl substances (PFASs) are substrates of the renal human organic anion transporter 4 (OAT4)
Authors: Louisse Jochem , Dellafiora Luca , van den Heuvel Jeroen J. M. W. , Rijkers Deborah , Leenders Liz , Dorne Jean-Lou C. M. , Punt Ans , Russel Frans G. M. , Koenderink Jan B.
Published: November 2022

Poly- and perfluoroalkyl substances (PFASs) are omnipresent in the environment and have been shown to accumulate in humans. Most PFASs are not biotransformed in animals and humans, so that elimination is largely dependent on non-metabolic clearance via bile and urine. Accumulation of certain PFASs in humans may relate to their reabsorption from the pre-urine by transporter proteins in the proximal tubules of the kidney, such as URAT1 and OAT4. The present study assessed the in vitro transport of 7 PFASs (PFHpA, PFOA, PFNA, PFDA, PFBS, PFHxS and PFOS) applying URAT1- or OAT4-transfected human embryonic kidney (HEK) cells. Virtually no transport of PFASs could be measured in URAT1-transfected HEK cells. All PFASs, except PFBS, showed clear uptake in OAT4-transfected HEK cells. In addition, these in vitro results were further supported by in silico docking and molecular dynamic simulation studies assessing transporter–ligand interactions. Information on OAT4-mediated transport may provide insight into the accumulation potential of PFASs in humans, but other kinetic aspects may play a role and should also be taken into account. Quantitative information on all relevant kinetic processes should be integrated in physiologically based kinetic (PBK) models, to predict congener-specific accumulation of PFASs in humans in a more accurate manner.
Characteristics of beer brewed with unconventional yeasts and addition of grape must, pulp and marc
Authors: Cioch-Skoneczny Monika , Królak Katarzyna , Tworzydło Zuzanna , Satora Paweł , Skoneczny Szymon
Published: November 2022

The aim of the paper was to determine potential of using grape pulp, marc and must in the beer production process. Samples were fermented using non-Saccharomyces yeasts (Dekkera bruxellensis 3429, Metschnikowia pulcherrima MG970690) and Saccharomyces cerevisiae Safale US-05 was used as a control. Grape marc was obtained by pressing grape must with a press. Subsequently, the grape marc, must and pulp were pasteurized and, together with wort, volumetrically introduced into fermentation flasks for fermentation. Mass changes taking place during the process were analyzed. Real extract, alcohol, free amino nitrogen (FAN) content, pH, color, sugars and organic acid profile were determined in obtained beers. The research has shown that the yeasts Dekkera bruxellensis 3429 fermented similarly to Saccharomyces cerevisiae Safale US-05. The yeast D. bruxellensis 3429 produced more alcohol in the finished beers in most cases and assimilated more FAN than M. pulcherrima MG970690. The D. bruxellensis 3429 strain most effectively used L-malic acid.
Characterisation of Planar Brownian Multiplicative Chaos
Authors: Jego Antoine
Published: November 2022

We characterise the multiplicative chaos measure $${\mathcal {M}}$$
M
associated to planar Brownian motion introduced in Bass et al. (Ann Probab 22(2):566–625, 1994), Aïdékon et al. (Ann. Probab. 48(4), 1785–1825, 2020) and Jego (Ann Probab 48(4):1597–1643, 2020) by showing that it is the only random Borel measure satisfying a list of natural properties. These properties only serve to fix the average value of the measure and to express a spatial Markov property. As a consequence of our characterisation, we establish the scaling limit of the set of thick points of planar simple random walk, stopped at the first exit time of a domain, by showing the weak convergence towards $${\mathcal {M}}$$
M
of the point measure associated to the thick points. In particular, we obtain the convergence of the appropriately normalised number of thick points of random walk to a nondegenerate random variable. The normalising constant is different from that of the Gaussian free field, as conjectured in Jego (Electron J Probab 25:39, 2020). These results cover the entire subcritical regime. A key new idea for this characterisation is to introduce measures describing the intersection between different independent Brownian trajectories and how they interact to create thick points.
Mathematical Structures of Non-perturbative Topological String Theory: From GW to DT Invariants
Authors: Alim Murad , Saha Arpan , Teschner Jörg , Tulli Iván
Published: November 2022

We study the Borel summation of the Gromov–Witten potential for the resolved conifold. The Stokes phenomena associated to this Borel summation are shown to encode the Donaldson–Thomas (DT) invariants of the resolved conifold, having a direct relation to the Riemann–Hilbert problem formulated by Bridgeland (Invent Math 216(1), 69–124, 2019). There exist distinguished integration contours for which the Borel summation reproduces previous proposals for the non-perturbative topological string partition functions of the resolved conifold. These partition functions are shown to have another asymptotic expansion at strong topological string coupling. We demonstrate that the Stokes phenomena of the strong-coupling expansion encode the DT invariants of the resolved conifold in a second way. Mathematically, one finds a relation to Riemann–Hilbert problems associated to DT invariants which is different from the one found at weak coupling. The Stokes phenomena of the strong-coupling expansion turn out to be closely related to the wall-crossing phenomena in the spectrum of BPS states on the resolved conifold studied in the context of supergravity by Jafferis and Moore (Wall crossing in local Calabi Yau manifolds, arXiv:0810.4909, 2008).
K-stability and birational models of moduli of quartic K3 surfaces
Authors: Ascher Kenneth , DeVleming Kristin , Liu Yuchen
Published: November 2022

We show that the K-moduli spaces of log Fano pairs$$({\mathbb {P}}^3, cS)$$(P3,cS)whereSis a quartic surface interpolate between the GIT moduli space of quartic surfaces and the Baily–Borel compactification of moduli of quartic K3 surfaces ascvaries in the interval (0, 1). We completely describe the wall crossings of these K-moduli spaces. As the main application, we verify Laza–O’Grady’s prediction on the Hassett–Keel–Looijenga program for quartic K3 surfaces. We also obtain the K-moduli compactification of quartic double solids, and classify all Gorenstein canonical Fano degenerations of$${\mathbb {P}}^3$$P3.
The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globe
Authors: Masunaga Aki , Mansfield Michael J. , Tan Yongkai , Liu Andrew W. , Bliznina Aleksandra , Barzaghi Paolo , Hodgetts Tamara L. , Ferrández-Roldán Alfonso , Cañestro Cristian , Onuma Takeshi A. , Plessy Charles , Luscombe Nicholas M.
Published: November 2022

Appendicularian tunicates are some of the most abundant mesozooplankton organisms with key roles in marine trophic webs and global carbon flux. Like most appendicularians with cosmopolitan distributions, Oikopleura dioica Fol, 1872 is considered a single species worldwide based on morphological features that distinguish them from other appendicularians. Despite their abundance, however, there are still only ~ 70 described appendicularian species, compared to over 2800 ascidian tunicates. Here we perform a molecular phylogenetic, morphological, and reproductive assessment of O. dioica specimens collected from the Ryukyu Archipelago, mainland Japan, and Europe. The specimens are morphologically very similar, with only detailed examination of the oikoplastic epithelium and quantitative measurements revealing minor distinguishing characteristics. Phylogenetic analyses of the ribosomal gene loci and mitochondrial cytochrome oxidase I (COI) gene strongly indicate that they form three separate genetic clades despite their morphological similarities. Finally, in vitro crosses between the Ryukyu and mainland Japanese specimens show total prezygotic reproductive isolation. Our results reveal that the current taxonomic O. dioica classification likely hides multiple cryptic species, highlighting the genetic diversity and complexity of their population structures. Cryptic organisms are often hidden under a single species name because their morphological similarities make them difficult to distinguish and their correct identification is fundamental to understanding Earth’s biodiversity. O. dioica is an attractive model to understand how morphological conservation can be maintained despite pronounced genetic divergence.
PD-1 expression, among other immune checkpoints, on tumor-infiltrating NK and NKT cells is associated with longer disease-free survival in treatment-naïve CRC patients
Authors: Al-Mterin Mohammad A. , Murshed Khaled , Elkord Eyad
Published: November 2022

A variety of variables, such as microsatellite instability or inflammatory mediators, are critical players in the development and progression of colorectal cancer (CRC). Natural killer (NK) and natural killer T (NKT) cells are involved in the prognoses of CRC. Immunological components of the tumor microenvironment (TME) impact cancer progression and therapeutic responses. We report that CRC patients with higher frequencies of tumor-infiltrating PD-1+ NK and NKT cells had significantly longer disease-free survival (DFS) than patients with lower frequencies. In agreement with that, patients with higher frequencies of tumor-infiltrating PD-1− NK and NKT cells showed shorter DFS. There were no significant associations between tumor-infiltrating PD-1+TIM-3+, PD-1+TIGIT+, PD-1+ICOS+, PD-1+LAG-3+ NK cells, and PD-1+TIM-3+, PD-1+TIGIT+, and PD-1+LAG-3+ NKT cells with DFS. This study highlights the significance of PD-1 expression on tumor-infiltrating NK and NKT cells and its association with disease prognoses in CRC patients.
Comparison of the Clinicopathological Characteristics and Prognosis of Esophageal Squamous Cell Carcinoma with Intramural Metastases and Multiple Primary Carcinomas
Authors: Cheng Na , Li Weihua , Wang Bingzhi , Li Zhuo , Yang Zhaoyang , Chen Rongshan , Xue Liyan
Published: November 2022

Background

It is difficult to distinguish esophageal squamous cell carcinoma with intramural metastases (IM) from multiple primary oesophageal carcinoma (MPEC). Nevertheless, there are significant differences in their prognoses and treatments. Therefore, our study aims to clarify the clinicopathological and prognostic characteristics of these two entities and to provide clues for differential diagnosis.

Methods

We retrospectively analyzed 6304 patients who underwent esophagectomy without neoadjuvant therapy. The clinicopathological and prognostic features of patients with IM and MPEC were evaluated. P53 and Rb1 were detected by immunohistochemical (IHC) staining using a tissue microarray.

Results

Among the 6304 patients, 127 (2.0%) had IM, and 138 (2.2%) had MPEC. Patients with IM were more likely to have an advanced pT (p < 0.001), pN (p < 0.001), more lymphovascular invasion (p < 0.001) and neural invasion (p < 0.001). Additionally, patients with IM had an extremely poor prognosis compared to those with MPEC, with 5‐year overall survival (OS) rates of 18.9% and 56.9%, respectively. Meanwhile, IM was found to be an independent poor prognostic indicator for OS and DFS. In the IM group, all patients showed consistent p53 expression in both primary and IM foci. Of note, Rb1 loss was found in 3 pairs of primary foci and metastases, along with p53 nonsense mutation.

Conclusions

Patients with IM had more risk factors and extremely worse prognosis than those with MPEC. It is essential to discriminate IM from MPEC when managing multifocal carcinomas. IHC staining of p53 and Rb1 may aid in differential diagnosis.
3D CRANI, a novel MR neurography sequence, can reliable visualise the extraforaminal cranial and occipital nerves
Authors: Casselman Jan , Van der Cruyssen Fréderic , Vanhove Frédéric , Peeters Ronald , Hermans Robert , Politis Constantinus , Jacobs Reinhilde
Published: November 2022

Objectives

We aim to validate 3D CRANI, a novel high-field STIR TSE, MR neurography sequence in the visualisation of the extraforaminal cranial and occipital nerve branches on a 3-T system. Furthermore, we wish to evaluate the role of gadolinium administration and calculate nerve benchmark values for future reference.

Methods

Eleven consecutive patients underwent MR imaging including the 3D CRANI sequence before and immediately after intravenous gadolinium administration. Two observers rated suppression quality and nerve visualisation using Likert scales before and after contrast administration. Extraforaminal cranial and occipital nerves were assessed. Nerve calibers and signal intensities were measured at predefined anatomical landmarks, and apparent signal intensity ratios were calculated.

Results

The assessed segments of the cranial and occipital nerves could be identified in most cases. The overall intrarater agreement was 79.2% and interrater agreement was 82.7% (intrarater κ = .561, p < .0001; interrater κ = .642, p < .0001). After contrast administration, this significantly improved to an intrarater agreement of 92.7% and interrater agreement of 93.6% (intrarater κ = .688, p < .0001; interrater κ = .727, p < .0001).

Contrast administration improved suppression quality and significant changes in nerve caliber and signal intensity measurements. Nerve diameter and signal intensity benchmarking values were obtained.

Conclusion

3D CRANI is reliable for the visualization of the extraforaminal cranial and occipital nerves. Intravenous gadolinium significantly improves MR neurography when applying this sequence. Benchmarking data are published to allow future assessment of the 3D CRANI sequence in patients with pathology of the extraforaminal cranial and occipital nerves.

Key Points

• MR neurography using the 3D CRANI sequence is a reliable method to evaluate the extraforaminal cranial and occipital nerves.

• Gadolinium contrast administration significantly improves suppression quality and nerve visualisation.

• Benchmarking values including apparent signal intensity ratios and nerve calibers depend on contrast administration and might play an important role in future studies evaluating extraforaminal cranial and occipital neuropathies.
Singular Value Perturbation and Deep Network Optimization
Authors: Riedi Rudolf H. , Balestriero Randall , Baraniuk Richard G.
Published: November 2022

We develop new theoretical results on matrix perturbation to shed light on the impact of architecture on the performance of a deep network. In particular, we explain analytically what deep learning practitioners have long observed empirically: the parameters of some deep architectures (e.g., residual networks, ResNets, and Dense networks, DenseNets) are easier to optimize than others (e.g., convolutional networks, ConvNets). Building on our earlier work connecting deep networks with continuous piecewise-affine splines, we develop an exact local linear representation of a deep network layer for a family of modern deep networks that includes ConvNets at one end of a spectrum and ResNets, DenseNets, and other networks with skip connections at the other. For regression and classification tasks that optimize the squared-error loss, we show that the optimization loss surface of a modern deep network is piecewise quadratic in the parameters, with local shape governed by the singular values of a matrix that is a function of the local linear representation. We develop new perturbation results for how the singular values of matrices of this sort behave as we add a fraction of the identity and multiply by certain diagonal matrices. A direct application of our perturbation results explains analytically why a network with skip connections (such as a ResNet or DenseNet) is easier to optimize than a ConvNet: thanks to its more stable singular values and smaller condition number, the local loss surface of such a network is less erratic, less eccentric, and features local minima that are more accommodating to gradient-based optimization. Our results also shed new light on the impact of different nonlinear activation functions on a deep network’s singular values, regardless of its architecture.
Effect of pulmonary vein isolation on rotor/multiple wavelet dynamics in persistent atrial fibrillation, association with vagal response and implications for adjunctive ablation
Authors: Nishimura Asuka , Harada Masahide , Ashihara Takashi , Nomura Yoshihiro , Motoike Yuji , Koshikawa Masayuki , Ito Takehiro , Watanabe Eiichi , Ozaki Yukio , Izawa Hideo
Published: November 2022

Persistent atrial fibrillation (PeAF) may develop arrhythmogenic substrates of rotors/multiple wavelets. However, the ways in which pulmonary vein isolation (PVI) affects the dynamics of rotor/multiple wavelets in PeAF patients remain elusive. Real-time phase-mapping (ExTRa mapping, EXT) in the whole left atrium (LA) was performed during PeAF before and after PVI (n = 111). The percentage of time in which rotor/multiple wavelets (phase singularities) was observed during each 5-s phase-mapping recording (non-passive activation ratio, %NP) was measured as an index of its burden. The mapping areas showing %NP ≥ 50% were defined as rotor/multiple-wavelet substrates (RSs). Before PVI, RSs were globally distributed in the LA. After PVI, %NP decreased (< 50%) in many RSs (PVI-modifiable RSs) but remained high (≥ 50%) in some RSs, especially localized in the anterior/septum/inferior regions (PVI-unmodifiable RSs, 2.3 ± 1.0 areas/patient). Before PVI, vagal response (VR) to high-frequency stimulation was observed in 23% of RSs, especially localized in the inferior region. VR disappearance after PVI was more frequently observed in PVI-modifiable RSs (79%) than in PVI-unmodifiable RSs (55%, p < 0.05), suggesting that PVI affects autonomic nerve activities and rotor/multiple wavelet dynamics. PVI-unmodifiable RSs were adjunctively ablated in 104 patients. The 1-year AT/AF-free survival rate was 70% in those with PVI alone (n = 115), and 86% in patients with the adjunctive ablation (log-rank test = 7.65, p < 0.01). PVI suppresses not only ectopic firing but also rotor/multiple wavelets partly via modification of autonomic nerve activities. The adjunctive ablation of PVI-unmodifiable RSs improved the outcome in PeAF patients and might be a novel ablation strategy beyond PVI.
Autopsy-based histopathological characterization of myocarditis after anti-SARS-CoV-2-vaccination
Authors: Schwab Constantin , Domke Lisa Maria , Hartmann Laura , Stenzinger Albrecht , Longerich Thomas , Schirmacher Peter
Published: November 2022

Cases of myocarditis, diagnosed clinically by laboratory tests and imaging have been described in the context of mRNA-based anti-SARS-CoV-2 vaccination. Autopsy-based description of detailed histological features of vaccine-induced myocarditis is lacking. We describe the autopsy findings and common characteristics of myocarditis in untreated persons who received anti-SARS-CoV-2 vaccination. Standardized autopsies were performed on 25 persons who had died unexpectedly and within 20 days after anti-SARS-CoV-2 vaccination. In four patients who received a mRNA vaccination, we identified acute (epi-)myocarditis without detection of another significant disease or health constellation that may have caused an unexpected death. Histology showed patchy interstitial myocardial T-lymphocytic infiltration, predominantly of the CD4 positive subset, associated with mild myocyte damage. Overall, autopsy findings indicated death due to acute arrhythmogenic cardiac failure. Thus, myocarditis can be a potentially lethal complication following mRNA-based anti-SARS-CoV-2 vaccination. Our findings may aid in adequately diagnosing unclear cases after vaccination and in establishing a timely diagnosis in vivo, thus, providing the framework for adequate monitoring and early treatment of severe clinical cases.

Graphical abstract
Influence of fat-free mass index on the survival of patients with head and neck cancer
Authors: Lapornik Nina , Avramovič Brumen Brigita , Plavc Gaber , Strojan Primož , Rotovnik Kozjek Nada
Published: November 2022

Purpose

To determine whether muscle mass, defined by fat-free mass index (FFMI) measured with bioelectrical impedance analysis (BIA), is predictive of survival of head and neck squamous cell carcinoma (HNSCC) patients.

Methods

HNSCC patients treated between 2014 and 2018 at the Department for Nutrition of the Institute of Oncology Ljubljana were reviewed. The FFMI values from the pretreatment BIA measurements and pretreatment body mass index (BMI) were used to categorize patients into groups with low and normal muscle mass and BMI using the Global Leadership Initiative on malnutrition (GLIM) recommended cutoff values. The impact of FFMI on disease-free survival (DFS) and overall survival (OS) was determined.

Results

Of the 71 included patients, 31 (43.7%) had normal FFMI, and 40 (56.3%) had low FFMI, whereas 44 (62%) and 27 (38%) of the patients had normal and low BMI, respectively. Between FFMI and BMI values, a significant correlation was found (RP = 0.75, p < 0.001). Univariate regression analysis showed that FFMI (as a continuous variable) was of prognostic significance for OS (p = 0.039), which was confirmed by multivariate regression analysis (p = 0.029). The model where BMI replaced FFMI negated the prognostic value of BMI (as a continuous variable). Neither FFMI nor BMI was found to be a predictor of DFS on univariate or multivariate analysis.

Conclusions

In the present group of HNSCC patients, low FFMI adversely influenced OS, emphasizing the importance of using body composition measurement over BMI alone for pretreatment nutritional evaluation of these patients.
Association between lateral wall electrode array insertion parameters and audiological outcomes in bilateral cochlear implantation
Authors: Thimsen Vivian , Mantsopoulos Konstantinos , Liebscher Tim , Taha Lava , Eisenhut Felix , Iro Heinrich , Hoppe Ulrich , Hornung Joachim
Published: November 2022

Purpose

The aims of this study were to compare speech recognition at different postoperative times for both ears in bilaterally implanted patients and to assess the influence of the time of deafness, frequency-to-place mismatch, angular insertion depth (AID) and angular separation between neighbouring electrode contacts on audiometric outcomes.

Methods

This study was performed at an academic tertiary referral centre. A total of 19 adult patients (6 men, 13 women), who received sequential bilateral implantation with lateral wall electrode arrays, were analysed in retrospective. Statistical analysis was performed using two-sided t test, Wilcoxon test, median test, and Spearman’s correlation.

Results

Postlingually deafened patients (deafness after the age of 10) had a significantly better speech perception (WRS65[CI]) than the perilingually deafened subjects (deafness at the age of 1–10 years) (p < 0.001). Comparison of cochlear duct length between peri- and postlingually deafened subjects showed a slightly significantly smaller cochleae in perilingual patients (p = 0.045). No association between frequency-to-place mismatch as well as angular separation and speech perception could be detected. There was even no significant difference between the both ears in the intraindividual comparison, even if insertion parameters differed.

Conclusion

The exact electrode position seems to have less influence on the speech comprehension of CI patients than already established parameters as preoperative speech recognition or duration of deafness.
Onset and mortality of Parkinson’s disease in relation to type II diabetes
Authors: Pezzoli Gianni , Cereda Emanuele , Amami Paolo , Colosimo Santo , Barichella Michela , Sacilotto Giorgio , Zecchinelli Anna , Zini Michela , Ferri Valentina , Bolliri Carlotta , Calandrella Daniela , Bonelli Maria Grazia , Cereda Viviana , Reali Elisa , Caronni Serena , Cassani Erica , Canesi Margherita , del Sorbo Francesca , Soliveri Paola , Zecca Luigi , Klersy Catherine , Cilia Roberto , Isaias Ioannis U.
Published: November 2022

Objectives

There is growing evidence that Parkinson’s disease and diabetes are partially related diseases; however, the association between the two, and the impact of specific treatments, are still unclear. We evaluated the effect of T2D and antidiabetic treatment on age at PD onset and on all-cause mortality.

Research design and methods

The standardized rate of T2D was calculated for PD patients using the direct method and compared with subjects with essential tremor (ET) and the general Italian population. Age at onset and survival were also compared between patients without T2D (PD-noT2D), patients who developed T2D before PD onset (PD-preT2D) and patients who developed T2D after PD onset (PD-postT2D).

Results

We designed a retrospective and prospective study. The T2D standardized ratio of PD (N = 8380) and ET (N = 1032) patients was 3.8% and 6.1%, respectively, while in the Italian general population, the overall prevalence was 5.3%. In PD-preT2D patients, on antidiabetic treatment, the onset of PD was associated with a + 6.2 year delay (p < 0.001) while no difference was observed in PD-postT2D. Occurrence of T2D before PD onset negatively affected prognosis (adjusted hazard ratio = 1.64 [95% CI 1.33–2.02]; p < 0.001), while no effect on survival was found in PD-postT2D subjects (hazard ratio = 0.86, [95% CI 0.53–1.39]; p = 0.54).

Conclusions

T2D, treated with any antidiabetic therapy before PD, is associated with a delay in its onset. Duration of diabetes increases mortality in PD-preT2D, but not in PD-postT2D. These findings prompt further studies on antidiabetic drugs as a potential disease-modifying therapy for PD.
Test–retest reliability of a 30-min fixed perceived effort cycling exercise
Authors: O’Malley Callum A. , Fullerton Christopher L. , Mauger Alexis R.
Published: November 2022

Purpose

Using exercise protocols at a fixed rating of perceived effort (RPE) is a useful method for exploring the psychophysical influences on exercise performance. However, studies that have employed this protocol have arbitrarily selected RPE values without considering how these values correspond to exercise intensity thresholds and domains. Therefore, aligning RPE intensities with established physiological thresholds seems more appropriate, although the reliability of this method has not been assessed.

Methods

Eight recreationally active cyclists completed two identical ramped incremental trials on a cycle ergometer to identify gas exchange threshold (GET). A linear regression model plotted RPE responses during this test alongside gas parameters to establish an RPE corresponding to GET (RPEGET) and 15% above GET (RPE+15%GET). Participants then completed three trials at each intensity, in which performance, physiological, and psychological measures were averaged into 5-min time zone (TZ) intervals and 30-min ‘overall’ averages. Data were assessed for reliability using intraclass correlation coefficients (ICC) and accompanying standard error measurements (SEM), 95% confidence intervals, and coefficient of variations (CoV).

Results

All performance and gas parameters showed excellent levels of test–retest reliability (ICCs =  > .900) across both intensities. Performance, gas-related measures, and heart rate averaged over the entire 30-min exercise demonstrated good intra-individual reliability (CoV =  < 5%).

Conclusion

Recreationally active cyclists can reliably replicate fixed perceived effort exercise across multiple visits when RPE is aligned to physiological thresholds. Some evidence suggests that exercise at RPE+15%GET is more reliable than RPEGET.
COVID-19 related decline in cancer screenings most pronounced for elderly patients and women in Germany: a claims data analysis
Authors: Muschol Jennifer , Strauss Cornelia , Gissel Christian
Published: November 2022

Purpose

This study aimed to analyze the utilization of cancer screenings in Germany before and during the COVID-19 pandemic in 2020. The objective of the analysis was to identify the population at particular risk and to derive recommendations for the future use of resources to prevent long-term deteriorations in health outcomes.

Methods

The analysis was conducted based on claims data of all preventive health services for 15,833,662 patients from the largest statutory health insurance fund in Germany. Utilization of general female cancer screening, general male cancer screening, general health checkup, colorectal cancer screening stool test, colorectal cancer screening consultation, colonoscopy, skin cancer screening, and mammography screening was compared before (2017–2019) and during (2020) the pandemic.

Results

Data of a total of 42,046,078 observed screenings showed that the utilization of the individual screenings developed differently, but that the overall utilization decreased significantly by 21.46% during the COVID-19 pandemic (p < 0.001). At the same time, no catch-up effects were detected for total screenings throughout the entire year 2020. The highest decline in screenings was found for the elderly (p < 0.001) and women (p < 0.001).

Conclusion

Because the elderly are at higher risk for cancer, the omission of early detection might lead to higher treatment costs, reduced quality of life, and higher mortality. In addition, women's medical care in particular has been negatively affected, for example, by the interruption of mammography screenings and the lack of catch-up effects. Therefore, resources must be targeted to reduce burdens on health outcomes and public health in the long term.
Prostaglandin E2 receptor EP1 expression in vulvar cancer
Authors: Buchholz Anna , Vattai Aurelia , Fürst Sophie , Vilsmaier Theresa , Zati Zehni Alaleh , Steger Alexander , Kuhn Christina , Schmoeckel Elisa , Dannecker Christian , Mahner Sven , Jeschke Udo , Heidegger Helene H.
Published: November 2022

Purpose

In recent years, incidence of vulvar cancer has been on the rise, whereas therapeutic options are still restricted. Therefore, new prognosticators and therapeutic targets are essential. Chronic inflammation plays an important role in carcinogenesis and COX-2, and its product prostaglandin E2 and its receptors EP1–4 are known to be important mediators in cancer initiation and progression.

Methods

EP1 expression in vulvar cancer specimens (n = 129) was investigated via immunohistochemistry and evaluated using the well-established immunoreactive score (IRS). Subsequently, the values were correlated with clinicopathological parameters.

Results

Our analysis did not reveal EP1 expression as a negative prognostic factor in overall and disease-free survival. However, in the subgroup of patients with lymph-node metastasis, overall survival was significantly shorter in tumors with high EP1 expression. Moreover, EP1 expression correlated positively with good differentiation of the tumor, but not with p16 status or COX-2 expression.

Conclusions

This study shed first light on EP1 expression in vulvar carcinoma. EP1 expression correlated significantly with the grading of the tumor, suggesting that it influences cell differentiation. Further research on EP1 signaling may lead to a deeper understanding of the molecular mechanisms of carcinogenesis.
Continuous successive cycles of biosorption and desorption of acid red 27 dye using water hyacinth leaves as an effective, economic, and ecofriendly biosorbent
Authors: Ramírez-Rodríguez Allan Eduardo , Cristiani-Urbina Eliseo , Morales-Barrera Liliana , Aranda-García Erick
Published: November 2022

We investigated the capacity of water hyacinth leaves (LEC) to biosorb 75 mg/L acid red 27 (AR27) in a continuous system comprising 30 successive biosorption/desorption cycles in a packed-bed column at pH 2.0 and 56.5 L/m2·h volumetric flux. Using 0.025 M NaHCO3 eluent at 113 L/m2·h volumetric flux, all the dye was desorbed (100% desorption efficiency) from the loaded LEC biomass within 5–6 h. The same biosorbent was used for 147.5 consecutive days. The AR27 biosorption capacity, breakthrough time, and exhaustion time decreased from 69.4 to 34.5 mg/g, 74.81 to 14.1 h, and 101.1 to 34.1 h, respectively, and the critical bed height increased from 1.04 to 2.35 cm, as the number of biosorption/desorption cycles increased from 1 to 30. LEC life factor based on biosorption capacity predicted that the packed bed would be exhausted after 51.95 cycles. LEC is a promising biosorbent for bioremediation of AR27-laden wastewaters.
Contribution of intrusive and symplastic growths in wood fibre tip development
Authors: Miodek Adam , Gizińska Aldona , Kojs Paweł
Published: November 2022

Key message

At initial stages of fibre development, radial enlargement of growing fibre tip is achieved, on average, in 37.8% intrusively and in 62.2% symplastically, whereas tip tangential enlargement is purely intrusive.

In this study, we have investigated the mode of growth of black locust wood fibre tips at initial stages of their development using detailed measurements. Growth of fibre tips may be considered in three directions: axial, tangential, and radial. An axial elongation of a fibre tip was described as intrusive and related to the separation of walls of neighbouring cells. However, determination of the contribution of intrusive vs. symplastic component of growth in tangential and radial directions was missing. Semi-thin transverse sections of the vascular cambium and adjacent tissues were obtained by ultramicrotome and stained with PAS and toluidine blue. An anatomical analysis of contribution of intrusive and symplastic growths in fibre tip radial and tangential enlargement was performed. Our study showed that during its development an average wood fibre tip grows only intrusively in tangential direction and shows intrusive-symplastic growth in radial direction. On average, at initial stages of its development, a fibre tip radial enlargement is achieved in 37.8% by intrusive growth and in 62.2% by coordinated (symplastic) growth.
Comparison of hemodynamics and root configurations between remodeling and reimplantation methods for valve-sparing aortic root replacement: a pulsatile flow study
Authors: Seki Masahiro , Kunihara Takashi , Takada Jyunpei , Sasaki Kenichi , Kumazawa Ryo , Seki Hiroshi , Sasuga Saeko , Fukuda Hirotsugu , Umezu Mitsuo , Iwasaki Kiyotaka
Published: November 2022

Purpose

To compare the characteristics of reimplantation (RI) using grafts with sinuses and remodeling (RM) with/without external suture annuloplasty using a pulsatile flow simulator.

Methods

Porcine aortic roots were obtained from an abattoir, and six models of RM and RI with sinuses were prepared. External suture annuloplasty (ESA) was performed in the RM models to decrease the root diameter to 22 mm (RM-AP22) and 18 mm (RM-AP18). Valve models were tested at mean pulsatile flow and aortic pressure of 5.0 L/min and 120/80 (100) mmHg, respectively, at 70 beats/min. The forward flow, regurgitation, leakage, backflow rates, valve-closing time, and mean and peak pressure gradient (p-PG) were evaluated. Root configurations were examined using micro-computed tomography (micro-CT).

Results

The backflow rate was larger in the RM models than in the RI models (RI: 8.56% ± 0.38% vs. RM: 12.64% ± 0.79%; p < 0.01). The RM-AP and RI models were comparable in terms of the forward flow, regurgitation, backflow rates, p-PG, and valve-closing time. The analysis using a micro-CT showed a larger dilatation of the sinus of the Valsalva in the RM groups than in the RI group (Valsalva: RI, 26.55 ± 0.40 mm vs. RM-AP22, 31.22 ± 0.55 mm [p < 0.05]; RM-AP18, 31.05 ± 0.85 mm [p < 0.05]).

Conclusions

RM with ESA and RI with neo-sinuses showed comparable hemodynamics. ESA to RM reduced regurgitation.
Radiological predictors of shunt response in the diagnosis and treatment of idiopathic normal pressure hydrocephalus: a systematic review and meta-analysis
Authors: Thavarajasingam Santhosh G. , El-Khatib Mahmoud , Vemulapalli Kalyan , Iradukunda Hector A. Sinzinkayo , K. Sajeenth Vishnu , Borchert Robin , Russo Salvatore , Eide Per K.
Published: November 2022

Background

Patients with the dementia subtype idiopathic normal pressure hydrocephalus (iNPH) may improve clinically following cerebrospinal fluid (CSF) diversion (shunt) surgery, though the predictors of shunt response remain debated. Currently, radiological features play an important role in the diagnosis of iNPH, but it is not well established which radiological markers most precisely predict shunt responsive iNPH.

Objective

To conduct a systematic review and meta-analysis to identify radiological predictors of shunt responsiveness, evaluate their diagnostic effectiveness, and recommend the most predictive radiological features.

Methods

Embase, MEDLINE, Scopus, PubMed, Google Scholar, and JSTOR were searched for original studies investigating radiological predictors of shunt response in iNPH patients. Included studies were assessed using the ROBINS-1 tool, and eligible studies were evaluated using a univariate meta-analysis.

Results

Overall, 301 full-text papers were screened, of which 28 studies were included, and 26 different radiological features were identified, 5 of these met the inclusion criteria for the meta-analysis: disproportionately enlarged subarachnoid space (DESH), callosal angle, periventricular white matter changes, cerebral blood flow (CBF), and computerized tomography cisternography. The meta-analysis showed that only callosal angle and periventricular white matter changes significantly differentiated iNPH shunt responders from non-responders, though both markers had a low diagnostic odds ratio (DOR) of 1.88 and 1.01 respectively. None of the other radiological markers differentiated shunt responsive from shunt non-responsive iNPH.

Conclusion

Callosal angle and periventricular changes are the only diagnostically effective radiological predictors of shunt responsive iNPH patients. However, due to the DORs approximating 1, they are insufficient as sole predictors and are advised to be used only in combination with other diagnostic tests of shunt response. Future research must evaluate the combined use of multiple radiological predictors, as it may yield beneficial additive effects that may allow for more robust radiological shunt response prediction.
Hotspot analysis of COVID-19 infection using mobile-phone location data
Authors: Kimura Yu , Seki Tatsunori , Miyata Satoshi , Arai Yusuke , Murata Toshiki , Inoue Hiroyasu , Ito Nobuyasu
Published: November 2022

Restrictions on outdoor activities are required to suppress the COVID-19 pandemic. To monitor social risks and control the pandemic through sustainable restrictions, we focus on the relationship between the number of people going out and the effective reproduction number. The novelty of this study is that we have considered influx population instead of staying-population, as the data represent congestion. This enables us to apply our analysis method to all meshes because the influx population may always represent the congestion of specific areas, which include the residential areas as well. In this study, we report the correlation between the influx population in downtown areas and business districts in Tokyo during the pandemic considering the effective reproduction number and associated time delay. Moreover, we validate our method and the influx population data by confirming the consistency of the results with those of the previous research and epidemiological studies. As a result, it is confirmed that the social risk with regard to the spread of COVID-19 infection when people travel to downtown areas and business districts is high, and the risk when people visit only residential areas is low.