bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying Artificial Neural Networks and Nonlinear Optimization Techniques to Fault Location in Transmission Lines—Statistical Analysis
Authors: Rocha Simone A. , Mattos Thiago G. , Cardoso Rodrigo T. N. , Silveira Eduardo G.
Published: June 2022

This study presents applications of artificial neural networks and nonlinear optimization techniques for fault location in transmission lines using simulated data in an electromagnetic transient program and actual data occurring in transmission lines. The localization is performed by a modular structure of 4 neural networks and by the minimization of objective functions descriptive of the problem, defined according to the parameters of the line and the type of short circuit, submitted to the methods Quasi-Newton, Ellipsoidal, and Real Polarized Genetic Algorithm. The results obtained are compared statistically with those of a classical analytical method. The analysis of the variance of location errors presented by the methods revealed, with 5% significance, statistical evidence that allowed the conclusion that the type of method used affects fault location indication. In simulated scenarios, minor errors were obtained with the neural network and larger with the analytical method. For field oscillographic, the largest errors were in the neural network; there is no evidence to reject the equality between the results of the analytical method and the nonlinear optimization techniques. The Tukey test identified no differences between the nonlinear optimization methods applied to the proposed objective functions, but the low computational cost associated with the Quasi-newton method highlights it. The nonlinear optimization methods used for the localization function proved to be promising for application in companies that operate electrical systems, providing localization errors similar to those presented by the classical analytical method.
Predictive Control of PV/Battery System under Load and Environmental Uncertainty
Authors: Batiyah Salem , Sharma Roshan , Abdelwahed Sherif , Alhosaini Waleed , Aldosari Obaid
Published: June 2022

The standalone microgrids with renewable energy resources (RERs) such as a photovoltaic (PV) system and fast changing loads face major challenges in terms of reliability and power management due to a lack of inherent inertial support from RERs and their intermittent nature. Thus, energy storage technologies such as battery energy storage (BES) are typically used to mitigate the power fluctuations and maintain a power balance in the system. This paper presents a model predictive control (MPC) based power management strategy (PMS) for such standalone PV/battery systems. The proposed method is equipped with an autoregressive integrated moving average (ARIMA) prediction method to forecast the load and environmental parameters. The proposed controller has the capabilities of (1) effective power management, (2) minimization of transients during disturbances, and (3) automatic switching of the operation of the PV between the maximum power point tracking (MPPT) mode and power-curtailed mode that prevents the overcharging of the battery and at the same time maximize the PV utilization. The effectiveness of the proposed method has been verified through a comprehensive simulation-based analysis.
Design of High-Dynamic PMSM Servo Drive Using Nonlinear Predictive Controller with Harmonic Disturbance Observer
Authors: Zhou Zhanqing , Yao Shuaijiang , Ma Chaolei , Zhang Guozheng , Geng Qiang
Published: June 2022

The high-dynamic permanent magnet (PM) motor servo system with high-bandwidth is the core equipment for industrial production, and the control bandwidth is also an important indexes to evaluate the performance of the servo system. The non-cascaded direct predictive speed control is an appropriate scheme to optimize the dynamic performance of the PM motor servo system. However, the high bandwidth of the non-cascaded control structure results in poor anti-interference ability, and it cannot effectively deal with the coupling relationship between current and speed, leading to poor control performance in the current limit region. Regarding the above problems, a nonlinear predictive speed control strategy combined with harmonic disturbance observer is proposed. In the proposed strategy, the disturbances of the servo system are separated from the mathematical model according to the nonlinear modeling theory, and the traditional disturbance observer is modified to estimate the harmonics. A nonlinear control law with strong disturbance suppression ability was designed. Furthermore, a complete current and speed prediction mechanism was introduced into the algorithm, in which the proportional differential (PD) controller is employed as the connection medium between the reference current and speed to solve the coupling problem of the non-cascaded control structure.
Pathogenesis of FGF23-Related Hypophosphatemic Diseases Including X-linked Hypophosphatemia
Authors: Nakanishi Tatsuro , Michigami Toshimi
Published: June 2022

Since phosphate is indispensable for skeletal mineralization, chronic hypophosphatemia causes rickets and osteomalacia. Fibroblast growth factor 23 (FGF23), which is mainly produced by osteocytes in bone, functions as the central regulator of phosphate metabolism by increasing the renal excretion of phosphate and suppressing the production of 1,25-dihydroxyvitamin D. The excessive action of FGF23 results in hypophosphatemic diseases, which include a number of genetic disorders such as X-linked hypophosphatemic rickets (XLH) and tumor-induced osteomalacia (TIO). Phosphate-regulating gene homologous to endopeptidase on the X chromosome (PHEX), dentin matrix protein 1 (DMP1), ectonucleotide pyrophosphatase phosphodiesterase-1, and family with sequence similarity 20c, the inactivating variants of which are responsible for FGF23-related hereditary rickets/osteomalacia, are highly expressed in osteocytes, similar to FGF23, suggesting that they are local negative regulators of FGF23. Autosomal dominant hypophosphatemic rickets (ADHR) is caused by cleavage-resistant variants of FGF23, and iron deficiency increases serum levels of FGF23 and the manifestation of symptoms in ADHR. Enhanced FGF receptor (FGFR) signaling in osteocytes is suggested to be involved in the overproduction of FGF23 in XLH and autosomal recessive hypophosphatemic rickets type 1, which are caused by the inactivation of PHEX and DMP1, respectively. TIO is caused by the overproduction of FGF23 by phosphaturic tumors, which are often positive for FGFR. FGF23-related hypophosphatemia may also be associated with McCune-Albright syndrome, linear sebaceous nevus syndrome, and the intravenous administration of iron. This review summarizes current knowledge on the pathogenesis of FGF23-related hypophosphatemic diseases.
Effects of Close-to-Nature Transformation on Soil Enzyme Activities and Organic Carbon Fractions in Cuninghamia lanceolata and Pinus massoniana Plantations
Authors: Shu Weiwei , Ming Angang , Zhang Jihui , Li Hua , Min Huilin , Ma Junxu , Yang Kun , Li Zhongguo , Zeng Ji , Wei Juling , Li Zhaoying , Tao Yi
Published: June 2022

Soil enzymes are involved in the process of mineralization of soil organic matters. The close-to-nature transformation (CNT) of plantations changes the soil enzyme activities by changing the composition of stand vegetation, which in turn affects the change process of soil organic carbon. We therefore selected two typical coniferous plantations in southwest China, Pinus massoniana and Cunninghamia lanceolate, to explore the effects of CNT on soil enzyme activities and soil organic carbon, and the relationship between them is explored through comparative study. Compared with control stands (CCK and PCK), CNT enhanced soil organic carbon; the content of water-soluble organic carbon in the 0–10 cm soil layer of the transformed C. lanceolata plantations (CCN) is 81.29% higher than those in the control stands (p < 0.05); the contents of particulate organic carbon and water-soluble organic carbon in the 10–30 cm soil layer are 95.42% and 48.68% higher than those in the control stands (p < 0.05), respectively; after the CNT, the protease, urease, and acid phosphatase in C. lanceolata plantations were higher than control stands, while protease and catalase in P. massoniana plantations were higher than control stands. Correlation analysis showed that catalase and protease were more closely related to organic carbon components than other organic enzymes; redundancy analysis (RDA) results show that pH and total nitrogen are key factors that cause changes in carbon fractions after the CNT. In general, CNT enhanced soil organic carbon in coniferous plantations, which was more conducive to soil organic carbon accumulation but had a negative effect on soil organic carbon stability to a certain extent. Therefore, the effect of tree species configuration on soil carbon stability components should be considered in the forest management practice.
Improving Ergometrine Production by easO and easP Knockout in Claviceps paspali
Authors: Qiao Yun-Ming , Wen Yan-Hua , Gong Ting , Chen Jing-Jing , Chen Tian-Jiao , Yang Jin-Ling , Zhu Ping
Published: June 2022

Ergometrine is widely used for the treatment of excessive postpartum uterine bleeding. Claviceps paspali is a common species for industrial production of ergometrine, which is often accompanied by lysergic acid α-hydroxyethylamide (LAH) and lysergic acid amide (LAA). Currently, direct evidence on the biosynthetic mechanism of LAH and LAA from lysergic acid in C. paspali is absent, except that LAH and LAA share the common precursor with ergometrine and LAA is spontaneously transformed from LAH. A comparison of the gene clusters between C. purpurea and C. paspali showed that the latter harbored the additional easO and easP genes. Thus, the knockout of easO and easP in the species should not only improve the ergometrine production but also elucidate the function. In this study, gene knockout of C. paspali by homologous recombination yielded two mutants ∆easOhetero-1 and ∆easPhetero-34 with ergometrine titers of 1559.36 mg∙L−1 and 837.57 mg∙L−1, which were four and two times higher than that of the wild-type control, respectively. While the total titer of LAH and LAA of ∆easOhetero-1 was lower than that of the wild-type control. The Aspergillus nidulans expression system was adopted to verify the function of easO and easP. Heterologous expression in A. nidulans further demonstrated that easO, but not easP, determines the formation of LAA.
Experimental Study on Performance of Transonic Compressor Cascade with Microgroove Polyurethane Coatings
Authors: Wang Liyue , Wang Cong , Qin Sheng , Lan Xinyue , Sun Gang , You Bo , Wang Meng , Zhong Yongjian , Hu Yan , Lu Huawei
Published: June 2022

Due to the harsh operating environment of aero-engines, a surface structure that provides excellent aerodynamic performance is urgently required to save energy and reduce emissions. In this study, microgroove polyurethane coatings fabricated by chemical synthesis are investigated in terms of their effect on aerodynamic performance, which is a new attempt to investigate the impact on aerodynamic performance of compressor cascade at transonic speeds. This method reduces manufacturing and maintenance cost significantly compared with traditional laser machining. Wake measurements are conducted in the high-speed linear compressor cascade wind tunnel to evaluate the performance of cascade attached with different microgroove polyurethane coatings. Compared with the Blank case, the microgroove polyurethane coatings have the characteristic of reducing flow loss, with a maximum reducing rate of 5.87% in the area-averaged total pressure loss coefficient. The mechanism of flow loss control is discussed through analyzing the correlation between the total pressure distribution and turbulence intensity distribution. The results indicate that a large quantity of energy loss in the flow field due to turbulence dissipation and the reduction in viscous drag by microgroove polyurethane coatings relates to its effect on turbulence control. This paper demonstrates a great perspective on designing micro-nano surface structure for aero-engine applications.
Multidimensional Vibro-Acoustical Diagnostics of Cavitation: Theory and Illustration on a Kaplan Turbine
Authors: Bajic Branko , Weissenberger Simon , Keller Markus
Published: June 2022

Korto’s multidimensional method for vibro-acoustical diagnostics and monitoring of turbine cavitation is based on a high number of spatially distributed sensors and the signal and data processing that systematically utilises three data dimensions: spatial, temporal, and operational. The method delivers unbiased data on cavitation intensity and rich diagnostical data on cavitation mechanisms. It is applicable on Kaplan, Francis, bulb, and reversible pump turbines, as well as pumps. In this paper, the theory of the method is introduced, and its application is illustrated on a prototype and three models of a Kaplan turbine. In the considered case, two distinct cavitation mechanisms responsible for the two erosion patches found in an overhaul are vibro-acoustically identified, quantified, and analysed. The cavitation quality of the models is compared. Cavitation as a source of vibration is discussed.
Regularization for a Sideways Problem of the Non-Homogeneous Fractional Diffusion Equation
Authors: Chen Yonggang , Qiao Yu , Xiong Xiangtuan
Published: June 2022

In this article, we investigate a sideways problem of the non-homogeneous time-fractional diffusion equation, which is highly ill-posed. Such a model is obtained from the classical non-homogeneous sideways heat equation by replacing the first-order time derivative by the Caputo fractional derivative. We achieve the result of conditional stability under an a priori assumption. Two regularization strategies, based on the truncation of high frequency components, are constructed for solving the inverse problem in the presence of noisy data, and the corresponding error estimates are proved.
Optical Properties and Upconversion Luminescence of BaTiO3 Xerogel Structures Doped with Erbium and Ytterbium
Authors: Lashkovskaya Ekaterina I. , Gaponenko Nikolai V. , Stepikhova Margarita V. , Yablonskiy Artem N. , Andreev Boris A. , Zhivulko Vadim D. , Mudryi Alexander V. , Martynov Igor L. , Chistyakov Alexander A. , Kargin Nikolai I. , Labunov Vladimir A. , Raichenok Tamara F. , Tikhomirov Sergey A. , Timoshenko Victor Yu.
Published: June 2022

Erbium upconversion (UC) photoluminescence (PL) from sol-gel derived barium titanate (BaTiO3:Er) xerogel structures fabricated on silicon, glass or fused silica substrates has been studied. Under continuous-wave excitation at 980 nm and nanosecond pulsed excitation at 980 and 1540 nm, the fabricated structures demonstrate room temperature PL with several bands at 410, 523, 546, 658, 800 and 830 nm, corresponding to the 2H9/2 → 4I15/2, 2H11/2 → 4I15/2, 4S3/2 → 4I15/2, 4F9/2→ 4I15/2 and 4I9/2→ 4I15/2 transitions of Er3+ ions. The intensity of erbium UC PL increases when an additional macroporous layer of strontium titanate is used beneath the BaTiO3 xerogel layer. It is also enhanced in BaTiO3 xerogel films codoped with erbium and ytterbium (BaTiO3:(Er,Yb)). For the latter, a redistribution of the intensity of the PL bands is observed depending on the excitation conditions. A multilayer BaTiO3:(Er,Yb)/SiO2 microcavity structure was formed on a fused silica substrate with a cavity mode in the range of 650–680 nm corresponding to one of the UC PL bands of Er3+ ions. The obtained cavity structure annealed at 450 °C provides tuning of the cavity mode by 10 nm in the temperature range from 20 °C to 130 °C. Photonic application of BaTiO3 xerogel structures doped with lanthanides is discussed.
Male Reproduction: Regulation, Differentiation and Epigenetics
Authors: Venditti Massimo , Minucci Sergio
Published: June 2022

The production of good-quality spermatozoa (SPZ) is one of the most intricate and far from being completely understood developmental processes during postnatal life [...]
Population Structure and Genetic Diversity of Chinese Honeybee (Apis Cerana Cerana) in Central China
Authors: Fang Fang , Chen Xiasang , Lv Jie , Shi Xinyan , Feng Xiaojuan , Wang Zhen , Li Xiang
Published: June 2022

Central China has a rich terrain with a temperate monsoon climate and varied natural environments for the Chinese honeybee (Apis cerana cerana). However, little comprehensive research on population genetic diversity has been done in this area. A population survey of the structure and genetic diversity of Apis cerana cerana in this area is deeply needed for understanding adaptation to variable environments and providing more references for the protection of honeybee biodiversity. In this study, we present a dataset of 72 populations of Chinese honeybees collected from nine sites by whole genome sequencing in Central China. We obtained 2,790,214,878 clean reads with an average covering a depth of 22×. A total of 27,361,052 single nucleotide polymorphisms (SNPs) were obtained by mapping to the reference genome with an average mapping rate of 93.03%. Genetic evolution analysis was presented via the population structure and genetic diversity based on the datasets of SNPs. It showed that Apis cerana cerana in plains exhibited higher genetic diversity than in mountain areas. The mantel test between Apis cerana cerana groups revealed that some physical obstacles, especially the overurbanization of the plains, contributed to the differentiation. This study is conducive to elucidating the evolution of Apis cerana in different environments and provides a theoretical basis for investigating and protecting the Chinese honeybee.
Upregulation of miR-33 Exacerbates Heat-Stress-Induced Apoptosis in Granulosa Cell and Follicular Atresia of Nile Tilapia (Oreochromis niloticus) by Targeting TGFβ1I1
Authors: Qiang Jun , Tao Fan-Yi , Lu Qi-Si , He Jie , Xu Pao
Published: June 2022

High temperature affects egg quality and increases follicular atresia in teleosts. The present study aimed to explore the regulated mechanism of ovary syndrome of Nile tilapia (Oreochromis niloticus) exposed to heat stress. To this end, we conducted histological and biochemical analyses and integrated miRNA-target gene analyses. The histochemical analyses confirmed that heat stress promoted the apoptosis of granulosa cell and therefore resulted in increased follicular atresia in the ovary. Heat stress led to the differential expression of multiple miRNAs (miR-27e, -27b-3p, -33, -34a -133a-5p, and -301b-5p). In a luciferase activity assay, miR-33 bound to the 3′-untranslated region (UTR) of the TGFβ1I1 (transforming growth factor-β1-induced transcript 1) gene and inhibited its expression. A TGFβ1I1 gene signal was detected in the granulosa cells of Nile tilapia by immunohistochemical analysis. Up-regulation of the miR-33 of tilapia at 6 d and 12 d exposed to heat (34.5 °C ± 0.5 °C) had significant down-regulation of the TGFβ1I1 expression of the gene and protein in tilapia ovaries. An miRNA-target gene integrated analysis revealed that miR-33 and TGFβ1I1 function in an apoptosis-related signal pathway. The signal transduction of the vascular endothelial growth factor (VEGF) family members VEGFA and its receptor (KDR) in the heat-stressed group decreased significantly compared with the control group. Transcript-levels of the Bax and Caspase-3 as apoptotic promotors were activated and Bcl-2 and Caspase-8 as apoptotic inhibitors were suppressed in the heat-stressed tilapia. These results suggest that heat stress increases the expression of miR-33, which targets TGFβ1I1 and inhibits its expression, resulting in decreased levels of follicle-stimulating hormone and 17β-estradiol and increased apoptosis by suppressing VEGF signaling, eventually inducing follicular atresia. In conclusion, our results show that the miR-33/TGFβ1I1 axis of Nile tilapia is involved in the follicular development of broodstock, and can suppress VEGF signaling to accelerate follicular atresia. Our findings demonstrate the suppressive role of miR-33 during oocyte development in Nile tilapia.
Patient Information and Informed Consent for Research in the Elderly: Lessons Learned from a Randomized Controlled Trial
Authors: López-Parra Maria , Zamora-Carmona Francesc , Sianes-Gallén Mònica , López-González Esmeralda , Gil-Rey Dolors , Costa-Ventura Helena , Borrás-Sánchez Miriam , Rayo-Posadas Gemma , Arizu-Puigvert Marta , Vives-Vilagut Roser
Published: June 2022

The informed consent (IC) of subjects participating in experimental studies is the mainstay to comply with the ethical principle of autonomy to ensure that the participation is voluntary. This experience was performed within the context of a single-center randomized clinical trial in elective prosthetic surgery. Obtaining IC in clinical trials is not without difficulties, and especially in the case of vulnerable populations it can be very challenging. This work aimed to identify the difficulties during the IC process for a clinical trial in subjects older than 65 years old and quantify and describe the use of IC in front of a witness. Methods: This is a mixed methodology study with a qualitative part (focus group with 4 nurses involved in the inclusion of subjects) and a quantitative part describing the characteristics of patients who signed IC forms. Results: The main difficulties identified are related to comprehension, sensory impairments, education level, and time. IC in front of witnesses was used in 20 patients out of 508. Conclusions: The participation of subjects older than 65 years old in clinical trials requires an adaptation of the process. The use of IC in front of a witness should always be considered in studies including elderly subjects.
Comparative Analysis of Endovascular Intervention and Endarterectomy in Patients with Femoral Artery Disease: A Systematic Review and Meta-Analysis
Authors: Ravikumar Nidhruv , Sreejith Gopika , Law Sharon Hiu Ching , Anand Prakhar , Varghese Noah , Kagdi Samrin , Kang Navneet , Nashnoush Mohamed , Salam Sihat , Ongidi Ibsen
Published: June 2022

Peripheral artery disease is a prevalent illness affecting more than 200 million people worldwide. A commonly used technique to manage the condition has been open endarterectomy. However, in recent times, a shift towards minimally invasive techniques has resulted in endovascular intervention as a popular alternative. This review aims to assess the safety and efficacy of endovascular intervention when compared with endarterectomy. A systematic review of the articles published in PubMed, Ovid, Embase, and Scopus within the last 10 years was conducted. The PRISMA guidelines were adhered to, and the Newcastle-Ottawa and NICE quality assessment scales were used. A meta-analysis of proportions was performed using the RStudio software (RStudio Team (2021). RStudio: Integrated Development Environment for R, PBC, Boston, MA, USA). Twenty-six studies were included, with a total of 7126 patients (endovascular, 2496; endarterectomy, 4630). Technical success was greater for endarterectomy than endovascular intervention with an odds ratio of 0.38; 95% CI [0.27–0.54]. In terms of safety as well endovascular intervention was better than endarterectomy with an odds ratio of 0.22; 95% CI [0.15 to 0.31] for wound infection. Endovascular intervention is a safe and effective procedure; however, it cannot be considered superior to endarterectomy.
Warm Autoimmune Hemolytic Anemia and Pure Red Cell Aplasia during a Severe COVID-19 B.1.1.7 Infection
Authors: Ghimire Anukul , Platnich Jaye , Chauhan Utkarsh
Published: June 2022

Warm autoimmune hemolytic anemia (AIHA) is a rare complication of COVID-19 infection. We report a case of warm AIHA in a patient with COVID-19 pneumonia treated with methylprednisolone and several red blood cell transfusions. Despite treatment of the warm AIHA, the patient’s reticulocyte count remained low, and his biochemical markers were suggestive of pure red cell aplasia, which was later attributed to a concurrent parvovirus B19 infection. This case highlights an unusual situation of two separate hematological processes caused by two separate and simultaneous viral infections.
Sustainable and Active Program—Development and Application of SAVING Methodology
Authors: Almeida-Silva Marina , Monteiro Ana , Carvalho Ana Rita , Teixeira Ana Marta , Moreira Jéssica , Tavares David , Tomás Maria Teresa , Coelho Andreia , Manteigas Vítor
Published: June 2022

The SAVING project aimed to create a sustainable and active aging program to promote the transition to sustainable aging in residential structures for the elderly (RSEs), developing research activities to apply the best strategies and good practices regarding the promotion of an active, healthy, and sustainable aging regarding social, economic, environmental, and pedagogic aspects. All this innovative methodology was built on a living-lab approach applied in one RSE, that was used as a case study. The results showed that the creation of the SAVING Brigade allowed not only increased reflection and mutual learning, but also created better conditions to face uncertainties and obstacles. Moreover, the use of indicators supported the basic themes and enabled comparison with other studies, between institutions or programs. Finally, the Action Plan acted as a tool for the development of previously defined strategies. It is possible to conclude that the breadth of the concept of quality of life encompasses the physical health of the individual, their psychological state, their social relationships, their perceptions, and the relationship with the characteristics of the context in which they are inserted. Therefore, active, sustainable, and healthy aging should be the goal.
Return to Work of Healthcare Workers after SARS-CoV-2 Infection: Determinants of Physical and Mental Health
Authors: Grazzini Maddalena , Lulli Lucrezia Ginevra , Mucci Nicola , Paolini Diana , Baldassarre Antonio , Gallinoro Veronica , Chiarelli Annarita , Niccolini Fabrizio , Arcangeli Giulio
Published: June 2022

Introduction. The SARS-CoV-2 pandemic has involved healthcare workers (HCWs) both as caregivers and as patients. This study is a retrospective cross-sectional analysis of the HCWs working in a third-level hospital in Central Italy who were infected with COVID-19 from March 2020 to April 2021. This research aims at identifying the physical and mental health outcomes of HCWs infected with COVID-19 who returned to work after the infection, the determinants of those outcomes, such as age and sex, and the identification of possible vulnerable professional groups. Methods. A questionnaire about the acute illness, the experience of returning to work, and health perceptions after the disease was administered to 427 healthcare workers 3 months after recovering from the SARS-CoV-2 infection. Results. The majority interviewed (84.5%) reported symptoms at the time of the positive test, with no significant differences regarding age or sex, while a significant difference in the mean age was found regarding hospitalization (p < 0.001). At 3 months after the infection, females (p = 0.001), older workers (p < 0.001), and healthcare assistants (p < 0.001) were more likely to report persistent symptoms. Sex (p = 0.02) and age (p = 0.006) influenced the quality of sleep after the infection. At work, the nurses group reported increase in workload (p = 0.03) and worse relationships (p = 0.028). At 3 months after the infection, female workers perceived worse physical (p = 0.002) and mental (p < 0.001) health status according to the SF-12. A negative correlation was found between age and PCS score (p < 0.001) but not MCS score (p = 0.86). A significant difference in PCS score was found between nurses and physicians (p = 0.04) and between residents and all other groups (p < 0.001). Finally, the group of workers reporting sleep alterations showed lower PCS and MCS scores (p < 0.001) and working relationships had an impact on MCS scores (p < 0.001). Conclusions. Age, sex, and type of job had an impact on physical and mental outcomes. Organizing specific interventions, also tailored to professional sub-groups, should be a target for healthcare systems to protect and boost the physical and mental health of their workers.
The Cost-Effectiveness Analysis of an Integrated Mental Health Care Programme in Germany
Authors: Mueller-Stierlin Annabel Sandra , Dinc Uemmueguelsuem , Herder Katrin , Walendzik Julia , Schuetzwohl Matthias , Becker Thomas , Kilian Reinhold
Published: June 2022

The network for mental health (NWpG = Netzwerk psychische Gesundheit) is an umbrella association for non-medical community mental health care facilities across Germany which are enabled to provide multi-professional mental health care packages including medical and psychosocial services reimbursed by German statutory health insurances since 2009. The aim of this study is to analyse the cost-effectiveness of providing NWpG mental health care packages plus treatment as usual (NWpG) to treatment as usual alone (TAU) in Germany. In a prospective, multicenter, controlled trial over 18 months, a total of 511 patients (NWpG = 251; TAU = 260) were observed in five regions, four times at six-month intervals. The EQ-5D-3L and the Client Sociodemographic and Service Receipt Inventory (CSSRI) were used to estimate quality-adjusted life-years and total costs of illness. Propensity score-adjusted cost–utility analysis was applied using the net benefit approach. No significant differences in costs and QALYs between NWpG and TAU groups were identified. The probability of NWpG being cost-effective compared to TAU was estimated below 75% for maximum willingness to pay (MWTP) values between 0 and 125,000 EUR. The additional provision of the NWpG package is not cost-effective compared to TAU alone.
The Phytomanagement of PFAS-Contaminated Land
Authors: Evangelou Michael W. H. , Robinson Brett H.
Published: June 2022

Globally, several hundred thousand hectares of both agricultural and urban land have become contaminated with per- and polyfluoroalkyl substances (PFAS). PFAS compounds are resistant to degradation and are mobile in soil compared to other common contaminants. Many compounds have KD values (matrix/solution concentration quotients) of <10. PFAS compounds endanger the health of humans and ecosystems by leaching into groundwater, exposure via dust, and, to a lesser extent, through plant uptake. This review aims to determine the feasibility of phytomanagement, the use of plants, and the use of soil conditioners to minimize environmental risk whilst also providing an economic return in the management of PFAS-contaminated land. For most sites, PFAS combinations render phytoextraction, the use of plants to remove PFAS from soil, inviable. In contrast, low Bioaccumulation Coefficients (BAC; plant and soil concentration quotients) timber species or native vegetation may be usefully employed for phytomanagement to limit human/food chain exposure to PFAS. Even with a low BAC, PFAS uptake by crop plants may still exceed food safety standards, and therefore, edible crop plants should be avoided. Despite this limitation, phytomanagement may be the only economically viable option to manage most of this land. Plant species and soil amendments should be chosen with the goal of reducing water flux through the soil, as well as increasing the hydrophobic components in soil that may bind the C-F-dominated tails of PFAS compounds. Soil conditioners such as biochar, with significant hydrophobic components, may mitigate the leaching of PFAS into receiving waters. Future work should focus on the interactions of PFAS with soil microbiota; secondary metabolites such as glomalin may immobilize PFAS in soil.
Green Credit and Total Factor Carbon Emission Performance—Evidence from Moderation-Based Mediating Effect Test
Authors: Cao Lingling , Niu Huawei
Published: June 2022

To achieve China’s new development pattern and the “dual carbon” goals, it is necessary to boost emission reduction and high-quality economic development simultaneously. Green credit (GC), consisting of environmental regulation and economic leverage, has a profound impact on improving total factor carbon emission performance (TFCEP). By selecting the panel data of 30 provinces and municipalities in China from 2001 to 2020, this paper constructs a series of panel models to analyze the transmission path of GC to TFCEP. The results indicate that the relationship between GC and TFCEP showed an “inverted-U-shaped” relationship. This is mainly because “energy-saving and emission reduction” first appeared in the government planning outline in 2006, and transition-friendly enterprises successfully transformed with low-interest green credit, thereby effectively improving their TFCEP. However, as environmental regulations continue to increase and the scale of green credit continues to expand, the efficiency of green credit allocation and internal conflicts with other environmental regulation policies are also emerging. At the same time, the advancement of industrial structure and green technology innovation had a significant mediating effect between GC and TFCEP; government quality has a strong moderating effect on the second stage of the mediating process. When GC reaches a certain scale, it tends to restrain TFCEP more in central and western China than in eastern China. Therefore, it is of great significance to continuously increase the scale of GC, promote the advancement of clean energy industrial structure, and improve green technology innovation.
Resilience, Occupational Stress, Job Satisfaction, and Intention to Leave the Organization among Nurses and Midwives during the COVID-19 Pandemic
Authors: Piotrowski Andrzej , Sygit-Kowalkowska Ewa , Boe Ole , Rawat Samir
Published: June 2022

The current study on the intention to leave the organization among nurses and midwives aligns with the broader direction of research on the consequences of demanding jobs. This is particularly important in the context of the COVID-19 pandemic, which began in 2020 and is ongoing. The aim of the current study was to identify the levels of intention to leave the organization and job satisfaction in a sample of 390 Polish nurses and midwives. A multiple stepwise linear regression was carried out to establish which variables are predictors of job satisfaction and intention to leave the organization. The following measures were used in the study: Nurses’ Occupational Stressor Scale, The Brief Resilience Coping Scale, The Turnover Intention Scale, The Job Satisfaction Scale, and an occupational questionnaire (number of workplaces, weekly number of evening and night shifts, working at a unit dedicated to treating COVID-19, working as a supervisor/executive). The current study showed that almost 25% of the sample reported high turnover intention, and a similar proportion reported low job satisfaction. Resilience was related to nurses’ job satisfaction. In the predictive models for job satisfaction, the organizational factor of the number of workplaces was significant (positively related), while job experience was a negative predictor of intention to leave. The practical implications of the results and the need to continue research on this topic are also discussed.
A Community-Led Approach as a Guide to Overcome Challenges for Therapy Research in Congenital Disorders of Glycosylation
Authors: Francisco Rita , Brasil Sandra , Pascoal Carlota , Edmondson Andrew C. , Jaeken Jaak , Videira Paula A. , de Freitas Cláudia , Ferreira Vanessa dos Reis , Marques-da-Silva Dorinda
Published: June 2022

Congenital Disorders of Glycosylation (CDG) are a large family of rare genetic diseases for which effective therapies are almost nonexistent. To better understand the reasons behind this, to analyze ongoing therapy research and development (R&D) for CDG, and to provide future guidance, a community-led mixed methods approach was organized during the 4th World Conference on CDG for Families and Professionals. In the quantitative phase, electronic surveys pointed to the prioritization of six therapeutic R&D tools, namely biobanks, registries, biomarkers, disease models, natural history studies, and clinical trials. Subsequently, in the qualitative phase, the challenges and solutions associated with these research tools were explored through community-driven think tanks. The multiple challenges and solutions identified administrative/regulatory, communication, financial, technical, and biological issues, which are directly related to three fundamental aspects of therapy R&D, namely data, sample, and patient management. An interdependence was traced between the prioritized tools, with diagnosis and therapies acting as bidirectional triggers that fuel these interrelationships. In conclusion, this study’s pioneering and adaptable community-led methodology identified several CDG therapy R&D gaps, many common to other rare diseases, without easy solutions. However, the strong proactive attitude towards research, based on inclusive and international partnerships and involving all members of the CDG community, sets the direction for better future therapy R&D.
Musculoskeletal Disorders Associated with Occupational Driving: A Systematic Review Spanning 2006–2021
Authors: Pickard Olivia , Burton Peta , Yamada Hayato , Schram Ben , Canetti Elisa F. D. , Orr Robin
Published: June 2022

Several occupations require workers to spend long periods of time driving road vehicles. This occupational task is associated with musculoskeletal disorders. The purpose of this review was to collate, synthesize, and analyze research reporting on musculoskeletal disorders associated with occupational driving, in order to develop a volume of evidence to inform occupational disorder mitigation strategies. A systematic search of academic databases (PubMed, EBSCO host, CINAHL, SPORTDiscus, and Web of Science) was performed using key search terms. Eligible studies were critically appraised using the Joanna Briggs Institute critical appraisal checklists. A Cohen’s kappa analysis was used to determine interrater agreement between appraisers. Of the 18,254 identified studies, 25 studies were selected and appraised. The mean critical appraisal score is 69% (range 38–100%), with a fair level of agreement (k = 0.332). The studies report that musculoskeletal disorders, most commonly lower back pain, is of concern in this population, particularly in truck, bus, and taxi drivers. Risk factors for these occupations include long hours in a sitting position, years in the profession, vehicle ergonomics, and vibration.
Potential of Aqueous Humor as a Liquid Biopsy for Uveal Melanoma
Authors: Im Deborah H. , Peng Chen-Ching , Xu Liya , Kim Mary E. , Ostrow Dejerianne , Yellapantula Venkata , Bootwalla Moiz , Biegel Jaclyn A. , Gai Xiaowu , Prabakar Rishvanth K. , Kuhn Peter , Hicks James , Berry Jesse L.
Published: June 2022

Tumor biopsy can identify prognostic biomarkers for metastatic uveal melanoma (UM), however aqueous humor (AH) liquid biopsy may serve as an adjunct. This study investigated whether the AH of UM eyes has sufficient circulating tumor DNA (ctDNA) to perform genetic analysis. This is a case series of 37 AH samples, taken before or after radiation, and one tumor wash sample, from 12 choroidal and 8 ciliary body (CB) melanoma eyes. AH was analyzed for nucleic acid concentrations. AH DNA and one tumor wash sample underwent shallow whole-genome sequencing followed by Illumina sequencing to detect somatic copy number alterations (SCNAs). Four post-radiation AH underwent targeted sequencing of BAP1 and GNAQ genes. Post-radiation AH had significantly higher DNA and miRNA concentrations than paired pre-radiation samples. Highly recurrent UM SCNAs were identified in 0/11 post-radiation choroidal and 6/8 post-radiation CB AH. SCNAs were highly concordant in a CB post-radiation AH with its matched tumor (r = 0.978). BAP1 or GNAQ variants were detected in 3/4 post-radiation AH samples. AH is a source of ctDNA in UM eyes, particularly in post-radiation CB eyes. For the first time, UM SCNAs and mutations were identified in AH-derived ctDNA. Suggesting that AH can serve as a liquid biopsy for UM.
Soluble Epoxide Hydrolase and Diabetes Complications
Authors: Anita Natasha Z. , Swardfager Walter
Published: June 2022

Type 2 diabetes mellitus (T2DM) can result in microvascular complications such as neuropathy, retinopathy, nephropathy, and cerebral small vessel disease, and contribute to macrovascular complications, such as heart failure, peripheral arterial disease, and large vessel stroke. T2DM also increases the risks of depression and dementia for reasons that remain largely unclear. Perturbations in the cytochrome P450-soluble epoxide hydrolase (CYP-sEH) pathway have been implicated in each of these diabetes complications. Here we review evidence from the clinical and animal literature suggesting the involvement of the CYP-sEH pathway in T2DM complications across organ systems, and highlight possible mechanisms (e.g., inflammation, fibrosis, mitochondrial function, endoplasmic reticulum stress, the unfolded protein response and autophagy) that may be relevant to the therapeutic potential of the pathway. These mechanisms may be broadly relevant to understanding, preventing and treating microvascular complications affecting the brain and other organ systems in T2DM.
Signaling Pathways Used by the Specialized Pro-Resolving Mediator Maresin 2 Regulate Goblet Cell Function: Comparison with Maresin 1
Authors: Olsen Markus V. , Lyngstadaas Anne V. , Bair Jeffrey A. , Hodges Robin R. , Utheim Tor P. , Serhan Charles N. , Dartt Darlene A.
Published: June 2022

Specialized pro-resolving mediators (SPMs), including Maresins (MaR)-1 and 2, contribute to tear film homeostasis and resolve conjunctival inflammation. We investigated MaR2′s signaling pathways in goblet cells (GC) from rat conjunctiva. Agonist-induced [Ca2+]i and high-molecular weight glycoconjugate secretion were measured. MaR2 increased [Ca2+]i and stimulated secretion. MaR2 and MaR1 stimulate conjunctival goblet cell function, especially secretion, by activating different but overlapping GPCR and signaling pathways, and furthermore counter-regulate histamine stimulated increase in [Ca2+]i. Thus, MaR2 and MaR1 play a role in maintaining the ocular surface and tear film homeostasis in health and disease. As MaR2 and MaR1 modulate conjunctival goblet cell function, they each may have potential as novel, but differing, options for the treatment of ocular surface inflammatory diseases including allergic conjunctivitis and dry eye disease. We conclude that in conjunctival GC MaR2 and MaR1, both increase the [Ca2+]i and stimulate secretion to maintain homeostasis by using one set of different, but overlapping, signaling pathways to increase [Ca2+]i and another set to stimulate secretion. MaR2 also resolves ocular allergy.
Advanced Glycations End Products in the Skin as Biomarkers of Cardiovascular Risk in Type 2 Diabetes
Authors: Planas Alejandra , Simó-Servat Olga , Hernández Cristina , Simó Rafael
Published: June 2022

The incidence and prevalence of diabetes are increasing worldwide, and cardiovascular disease (CVD) is the leading cause of death among subjects with type 2 diabetes (T2D). The assessment and stratification of cardiovascular risk in subjects with T2D is a challenge. Advanced glycation end products are heterogeneous molecules produced by non-enzymatic glycation of proteins, lipids, or nucleic acids. Accumulation of advanced glycation end products is increased in subjects with T2D and is considered to be one of the major pathogenic mechanism in developing complications in diabetes. Skin AGEs could be assessed by skin autofluorescence. This method has been validated and related to the presence of micro and macroangiopathy in individuals with type 2 diabetes. In this context, the aim of this review is to critically summarize current knowledge and scientific evidence on the relationship between skin AGEs and CVD in subjects with type 2 diabetes, with a brief reference to other diabetes-related complications.
Lipidomics of Bioactive Lipids in Alzheimer’s and Parkinson’s Diseases: Where Are We?
Authors: Chiurchiù Valerio , Tiberi Marta , Matteocci Alessandro , Fazio Federico , Siffeti Hasibullah , Saracini Stefano , Mercuri Nicola Biagio , Sancesario Giuseppe
Published: June 2022

Lipids are not only constituents of cellular membranes, but they are also key signaling mediators, thus acting as “bioactive lipids”. Among the prominent roles exerted by bioactive lipids are immune regulation, inflammation, and maintenance of homeostasis. Accumulated evidence indicates the existence of a bidirectional relationship between the immune and nervous systems, and lipids can interact particularly with the aggregation and propagation of many pathogenic proteins that are well-renowned hallmarks of several neurodegenerative disorders, including Alzheimer’s (AD) and Parkinson’s (PD) diseases. In this review, we summarize the current knowledge about the presence and quantification of the main classes of endogenous bioactive lipids, namely glycerophospholipids/sphingolipids, classical eicosanoids, pro-resolving lipid mediators, and endocannabinoids, in AD and PD patients, as well as their most-used animal models, by means of lipidomic analyses, advocating for these lipid mediators as powerful biomarkers of pathology, diagnosis, and progression, as well as predictors of response or activity to different current therapies for these neurodegenerative diseases.
The Therapeutic Treatment with the GAG-Binding Chemokine Fragment CXCL9(74–103) Attenuates Neutrophilic Inflammation and Lung Dysfunction during Klebsiella pneumoniae Infection in Mice
Authors: Boff Daiane , Russo Remo Castro , Crijns Helena , de Oliveira Vivian Louise Soares , Mattos Matheus Silvério , Marques Pedro Elias , Menezes Gustavo Batista , Vieira Angélica Thomaz , Teixeira Mauro Martins , Proost Paul , Amaral Flávio Almeida
Published: June 2022

Klebsiella pneumoniae is an important pathogen associated with hospital-acquired pneumonia (HAP). Bacterial pneumonia is characterized by a harmful inflammatory response with a massive influx of neutrophils, production of cytokines and chemokines, and consequent tissue damage and dysfunction. Targeted therapies to block neutrophil migration to avoid tissue damage while keeping the antimicrobial properties of tissue remains a challenge in the field. Here we tested the effect of the anti-inflammatory properties of the chemokine fragment CXCL9(74–103) in pneumonia induced by Klebsiella pneumoniae in mice. Mice were infected by intratracheal injection of Klebsiella pneumoniae and 6 h after infection were treated systemically with CXCL9(74–103). The recruitment of leukocytes, levels of cytokines and chemokines, colony-forming units (CFU), and lung function were evaluated. The treatment with CXCL9(74–103) decreased neutrophil migration to the airways and the production of the cytokine interleukin-1β (IL-1β) without affecting bacterial control. In addition, the therapeutic treatment improved lung function in infected mice. Our results indicated that the treatment with CXCL9(74–103) reduced inflammation and improved lung function in Klebsiella pneumoniae-induced pneumonia.