The Effect of Phenolic Extract of Mint (Mentha spicata) in the Fertility of Male Laboratory Mice.
Authors: Nassir Faisa Ahmed , sattar abdull jabar Ali Abdull , Almousswi Zain alabdeen 
Published: November 2023

The current study was conducted to investigate the effect of the phenolic extract
of the Mentha spicata plant on the fertility of laboratory mice. The phenolic
compounds were extracted using the Reflex condenser from the leaves of the
Mentha spicata plant, and it was chemically detected. In the current study,)Albino Mice of the musculus Mus type was used at an age ranging between (12-11)
months. The group was randomly divided into three groups. The control group
was injected with a physiological solution (0.9% NaCl), the second group was injected with the phenolic extract of the Mentha spicata plant at (100 mg/kg) concentration, and the third group was injected by concentration (200 mg/kg) from
the phenolic extract of the Mentha spicata. The phenols were dissolved in a physiological solution (0.9% NaCl), and all individuals of these groups were injected
through the peritoneal membrane I.P. at a rate of 0.5 ml per animal per day for 30
days. The phenolic extract of Mentha spicata leaves caused a significant decrease
(P<0.05) in the level of Testosterone and Luteinizing hormone (ng/ml) in the
blood serum of the first and second treatment group when compared with the
control group. The study also indicates a significant decrease (P<0.05) in the
concentration of Follicle-Stimulating Hormone in the third treatment group compared with its level in the control group. At the same time, there were no significant changes (P<0.05) for the second treatment group compared with the control
group. The average concentration of sperms taken from the caudal epididymis did
not change significantly (P<0.05) for both treatments compared to the control
group. The percentage of motile sperm decreased significantly (P<0.05) as a result of treatment with the phenolic extract of Mentha spicata leaves, and this percentage is directly proportional to the increase in the doses used of the extract.
Sperm vitality compared to the control group, as for the percentage leading to abnormal sperms, the results showed a significant increase (P<0.05) in the average
percentage of abnormal sperms in the caudal epididymis of the second treatment
animals when compared with the rates of the first and control groups, while there
was no There were no significant changes (P<0.05) in the rate of the first treatment compared to the control. Histologically, the results indicate that treatment
with phenolic extract of Mentha spicata leaves caused simple histological changes, represented by a simple vacuole of the interstitial layer between the tubules,
slight congestion and partial detachment of the germ cells from the basement
membrane with a thick fibrous capsule surrounding the epididymis tubes and
congestion of blood vessels. In contrast, the seminal tubules and seminal lumen
remained normal. This compared to the control group, which showed the semi-
Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.03.48 2
niferous tubules, seminal cavity and germ layers commonly with normal spermatozoa stages.
Keywords: Phenolic extract, Mentha spicata, Fertility