bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Effects of Vacuum Insulating Glazing Application for Reducing Greenhouse Gas Emission (GHGE) from Apartment Buildings in the Korean Capital Region
Authors: Baek Sanghoon , Kim Sangchul
Published: June 2020

Korea has set a goal of reducing greenhouse gas emissions (GHGEs) to levels 37% below the “business as usual (BAU)” level by 2030, and the building sector, in particular, aims to reduce GHGEs by 45,000,000-ton CO2-eq by 2020. In order to reach this goal, it is crucial to reduce GHGEs that result from energy consumption in apartment buildings, which account for approximately 65% of all buildings in the capital region where the population is concentrated. Moreover, as apartment buildings not only have high window-to wall area ratios, but also use insulating glazing (IG) with low thermal performance, an advanced window system with low heat transmittance (U-value), such as a concrete structure, is necessary for effective GHGE reduction. Therefore, this study aims to evaluate the GHGE reduction effects from replacing existing IG vacuum insulating glazing (VIG) with low U-values in the apartment housing located in the capital region. The analysis revealed the possibility of a GHGE reduction by 45%–79% with the application of commercial VIG with U-values of 0.7 W/m2·K in lieu of the existing IG with U-values ranging from 1.2 to 3.3 W/m2·K for all apartment buildings located in the capital region. Furthermore, GHGEs could be reduced by 82%–93% by replacing the existing IG with VIG with U-values of 0.2 W/m2·K.
Development of Optimal Conditioning Method to Improve Economic Efficiency of Polymer Electrolyte Membrane (PEM) Fuel Cells
Authors: Kim Min Soo , Song Joo Hee , Kim Dong Kyu
Published: June 2020

This study presents an economical conditioning method for polymer electrolyte membrane (PEM) fuel cells through a parametric study investigating the factors affecting online conditioning methods. First, we compared the operating conditions between constant current (CC) mode and constant voltage (CV) mode conditioning to understand the effects of current and potential differences on conditioning. We found that CV mode conditioning is at least one hour faster at the same load. This is because unlike CV mode conditioning, which has a constant load over the entire range of the membrane electrode assembly (MEA), CC mode conditioning features current flow through the existing passage of the pre-activated triple phase boundary of the MEA so that the electronic load is not entirely used in the conditioning process. Second, the optimization of CV mode conditioning was conducted by controlling the conditioning temperature. Lastly, the economics of the proposed method were analyzed by comparing it with existing conditioning methods. Using this optimal conditioning method can reduce the consumption of hydrogen during conditioning by ~87.5% compared to previous methods. The findings from this study provide the means to lower the actual production cost of fuel cells, thereby ensuring market access.
Monitoring Oxidative Status in Winemaking by Untargeted Linear Sweep Voltammetry
Authors: Jeremic Jelena , Ricci Arianna , Tacconi Gabriele , Lagarde-Pascal Christine , Parpinello Giuseppina Paola , Versari Andrea
Published: June 2020

An electrochemical portable device based on linear sweep voltammetry was evaluated for studying the redox behavior of polyphenolic compounds in industrial scale winemaking to infer the effects of selected early processing steps on the vinification trials of Pinot gris, Chardonnay, Vermentino and Sangiovese grapes. For each sample, the redox behavior showed a distinctive voltammetric signal pattern related to the processing step during winemaking, therefore being useful as a potential fingerprint for wine identification and to provide insights about the phenolic content. For instance, there was a high correlation (R2 = 0.72) between the total phenolic compounds (PhenOx) and the easily oxidizable compounds (EasyOx), the latter representing approx. 30% on average of the total phenolics. Furthermore, the maceration of red grapes was concluded after 29 days based on information driven by the phenolics pattern. As expected, during alcoholic fermentation, white wines showed a lower content of phenolic compounds than those found in red wines, with an average ratio PhenOx/EasyOx of about 4.7, 5.0 and 3.6 for Chardonnay, Pinot gris and Vermentino, respectively. The portable tool with miniaturized disposable electrodes showed interesting analytical features that can be exploited for on-site and real-time quality control for monitoring change in phenolic composition during wine processing and storage, and for tailoring winemaking practices to enhance the color stability of products.
3’-UTR Polymorphisms of Vitamin B-Related Genes Are Associated with Osteoporosis and Osteoporotic Vertebral Compression Fractures (OVCFs) in Postmenopausal Women
Authors: Ahn Tae-Keun , Kim Jung Oh , An Hui Jeong , Park Han Sung , Choi Un Yong , Sohn Seil , Kim Kyoung-Tae , Kim Nam Keun , Han In-Bo
Published: June 2020

As life expectancy increases, the prevalence of osteoporosis is increasing. In addition to vitamin D which is well established to have an association with osteoporosis, B vitamins, such as thiamine, folate (vitamin B9), and cobalamin (vitamin B12), could affect bone metabolism, bone quality, and fracture risk in humans by influencing homocysteine/folate metabolism. Despite the crucial role of B vitamins in bone metabolism, there are few studies regarding associations between B vitamin-related genes and osteoporosis. In this study, we investigated the genetic association of four single nucleotide polymorphisms (SNPs) within the 3’-untranslated regions of vitamin B-related genes, including TCN2 (encodes transcobalamin II), CD320 (encodes transcobalamin II receptor), SLC19A1 (encodes reduced folate carrier protein 1), and SLC19A2 (encodes thiamine carrier 1), with osteoporosis and osteoporotic vertebral compression fracture (OVCF). We recruited 301 postmenopausal women and performed genotyping of CD320 rs9426 C>T, TCN2 rs10418 C>T, SLC19A1 rs1051296 G>T, and SLC19A2 rs16862199 C>T using a polymerization chain reaction-restriction fragment length polymorphism assay. There was a significantly higher incidence of both osteoporosis (AOR 5.019; 95% CI, 1.533–16.430, p < 0.05) and OVCF (AOR, 5.760; 95% CI, 1.480–22.417, p < 0.05) in individuals with genotype CD320 CT+TT and high homocysteine concentrations. Allele combination analysis revealed that two combinations, namely CD320 C-TCN2 T-SLC19A1 T-SLC19A2 C (OR, 3.244; 95% CI, 1.478–7.120, p < 0.05) and CD320 T-TCN2 C-SLC19A1 G-SLC19A2 C (OR, 2.287; 95% CI, 1.094–4.782, p < 0.05), were significantly more frequent among the osteoporosis group. Our findings suggest that SNPs within the CD320 gene in 3´-UTR may contribute to osteoporosis and OVCF occurrences in some individuals. Furthermore, specific allele combinations of CD320, TCN2, SLC19A1, and SLC19A2 may contribute to increased susceptibility to osteoporosis and OVCF.
Short Term Effects of Livestock Manures on Soil Structure Stability, Runoff and Soil Erosion in Semi-Arid Soils under Simulated Rainfall
Authors: Goldberg Nurit , Nachshon Uri , Argaman Eli , Ben-Hur Meni
Published: June 2020

The long term effects of applying livestock manures as soil amendment are well known. However, these manures usually contain high soluble salts content, which could increase the soil salinity and sodicity within a short time after their application. The aim of this study was to investigate the short term effects of animal manure application on soil structure stability, infiltration rate (IR), and runoff and soil erosion formation under rainfall conditions. Two soils, a non-calcareous, sandy soil with 0.2% organic matter, and a calcareous, clayey soil with 4.7% organic matter were sampled from a semiarid region. The soils were mixed with raw cattle manure or with compost, and soils with no addition were considered as a control. The two soils with the three treatments were incubated for 21 days, and then subjected to 80 mm of simulated rainstorm. In contradiction to previous works, it was found that the manure reduced soil structure stability, reduced infiltration, increased surface runoff and led to soil loss. The negative impact of the raw manure on soil structure was stronger than that of the mature compost. The findings of this study indicate the high sensitivity of arable soils to erosion processes during the first few weeks following the addition of manure to the soil, and therefore could contribute to the decision-making process of the timing of manure application, namely to make sure that the manure is applied well before the rainy season, in order to avoid the aforementioned soil erosion.
Pneumoparotid and Pneumoparotitis: A Literary Review
Authors: Gazia Francesco , Freni Francesco , Galletti Cosimo , Galletti Bruno , Bruno Rocco , Galletti Cosimo , Meduri Alessandro , Galletti Francesco
Published: June 2020

Pneumoparotid is a rare condition of parotid swelling. The presence of the air in gland parenchyma is caused by an incompetent Stensen’s duct with high pressure may cause the acini’s rupture. We reviewed 49 manuscripts, from 1987 to today, that enrolled a total of 54 patients with pneumoparotid. Our review evaluated the following evaluation parameters: gender, age, etiology, clinical presentation, treatment, days of resolution after diagnosis, relapse and complications. The most frequent etiology is self-induction by swelling the cheeks (53.7%). This cause mainly involves children (74%), for conflicts with parents, excuses for not going to school, nervous tics or adults (16%) with psychiatric disorders. Iatrogenic causes are also frequent (16.6%), for dental treatments (55.5%) or use of continuous positive airway pressure (CPAP) (33.4%). Medical therapy is the most practiced (53.7%), in most cases it is combined with behavioral therapy (25.9%) or psychotherapy (25.9%). Surgery is rarely used (9.2%) as a definitive solution through parotidectomy (50%) or ligation of the duct (50%). The most common complication is subcutaneous emphysema (24.1%), sometimes associated with pneumomediastinum (5.5%). Careful treatment and management are necessary to ensure the resolution of the pathology and counteract the onset of complications.
Can Psychopathy Be Adaptive at Work? Development and Application of a Work Focused Self- and Other-Report Measure of the Triarchic Psychopathy Model
Authors: Sutton Anna , Roche Maree , Stapleton Madeleine , Roemer Anja
Published: June 2020

Psychopathy may have both adaptive and maladaptive effects at work but research into workplace psychopathy is constrained by the lack of short, work-relevant measures that can be used for both self- and other-report. We adapt the Triarchic Psychopathy Measure (TriPM) for this purpose and distinguish the (mal)adaptive effects of psychopathy at work in two time-lagged survey samples. Sample 1 consisted of managers reporting their psychopathic traits and work outcomes (well-being, engagement, burnout and job performance). Sample 2 reported on their managers’ psychopathic traits and leadership styles (servant and abusive supervision) and their own work outcomes. The TriPM (Work) is a reliable, valid, 21-item measure of triarchic psychopathy at work with self- and other-report forms. Using this measure, we demonstrate that the triarchic model’s boldness trait is related to servant leadership and predicts improved well-being and performance while meanness and disinhibition are related to abusive supervision and predict increased burnout.
Patterning in Patient Referral to and Uptake of a National Exercise Referral Scheme (NERS) in Wales from 2008 to 2017: A Data Linkage Study
Authors: Morgan Kelly , Rahman Muhammad , Moore Graham
Published: June 2020

Exercise referral schemes have shown small but positive impacts in randomized controlled trials (RCTs). Less is known about the long-term reach of scaled up schemes following a RCT. A RCT of the National Exercise Referral Scheme (NERS) in Wales was completed in 2010, and the scheme scaled up across Wales. In this study, using a retrospective data linkage design, anonymized NERS data were linked to routine health records for referrals between 2008 and 2017. Rates of referral and uptake were modelled across years and a multilevel logistic regression model examined predictors of uptake. In total, 83,598 patients have been referred to the scheme and 67.31% of eligible patients took up NERS. Older adults and referrals for a musculoskeletal or level four condition were more likely to take up NERS. Males, mental health referrals, non-GP referrals and those in the most deprived groupings were less likely to take up NERS. Trends revealed an overall decrease over time in referrals and uptake rates among the most deprived grouping relative to those in the least deprived group. Findings indicate a widening of inequality in referral and uptake following positive RCT findings, both in terms of patient socioeconomic status and referrals for mental health.
High-Intensity Training Reduces CVD Risk Factors among Rotating Shift Workers: An Eight-Week Intervention in Industry
Authors: Mamen Asgeir , Øvstebø Reidun , Sirnes Per Anton , Nielsen Pia , Skogstad Marit
Published: June 2020

Rotating shift work is associated with risk factors for cardiovascular disease (CVD). We have studied the effect of 17 min high-intensity training three times a week over eight weeks on CVD risk factors among shift workers. Sixty-five shift workers from two plants were recruited. They were all deemed healthy at the initial health screening and in 100% work. From plant A, 42 workers, and plant B, 23 workers participated. After the intervention, 56 workers were retested. The intervention group consisted of 19 participants from plant A who had participated in at least 10 sessions. Twenty workers from plant B and 17 workers from plant A that not had taken part in the training were included in the control group. All workers reported physical activity (PA) by questionnaires before and after the training intervention. We measured blood pressure, heart rate, lipids, glycated hemoglobin (HbA1c), and C-reactive protein (CRP) and arterial stiffness. Maximal oxygen uptake ( V . O2max) was assessed by bicycle ergometry. The intervention group favorably differed significantly from the control group in improvement of systolic and diastolic blood pressure and glycated hemoglobin (HbA1c). Short training sessions with 4 min of high-intensity PA, three times a week, for eight weeks among rotating shift workers reduced some CVD risk factors. PA interventions in occupational settings may thus decrease coronary heart disease and stroke incidences in this vulnerable group of workers.
Arsenic in Hair as a Marker of Exposure to Smoke from the Burning of Treated Wood in Domestic Wood Burners
Authors: Dirks Kim N. , Chester Alana , Salmond Jennifer A. , Talbot Nicholas , Thornley Simon , Davy Perry
Published: June 2020

Timber treated with the anti-fungal chemical copper chrome arsenate is used extensively in the New Zealand building industry. While illegal, the burning of treated timber is commonplace in New Zealand and presents a health risk. Outdoor ambient monitoring of arsenic in airborne particulate matter in New Zealand has identified levels that exceed the maximum standards of 5.5 ng m−3 (annual average) at some urban locations. In this study, two-week-old beard hair samples were collected during the winter months to establish individual exposure to arsenic using Inductively Coupled Plasma-Mass Spectrometry. These results were then compared with questionnaire data about wood burner use for the two weeks prior to sampling, and spatial trends in arsenic from ambient monitoring. Results suggest that the burning of construction timber that may contain arsenic is associated with a higher level of arsenic in hair than those who burn logs or coal exclusively. There is no association between the area-level density of wood burners and arsenic levels but a significant correlation with individual household choice of fuel as well as the smell of wood smoke in the community, suggesting very localised influences. Strategies are needed to raise awareness of the risks of burning treated timber and to provide economically-viable alternatives.
Perceptions and Attitudes of Gynecologic and Pediatric Professionals Regarding Dietary Exposure to Chemical Pollutants
Authors: Arrebola Juan Pedro , Muñoz Araceli , Ferrero Silvia , Larrea-Killinger Cristina
Published: June 2020

There is increasing concern regarding the potential implications of continuous dietary exposure to low doses of artificial chemical pollutants, particularly in critical life stages such as pregnancy and lactation. Within a wider social research, we analyzed the risk perception, discourses, and attitudes of health professionals regarding dietary exposure to artificial chemical contaminants. Data was collected by personal interviews on 35 health professionals from two Spanish regions. Although the participants’ discourses were strongly dominated by the nutritional composition and microbiological contamination, 34 expressed some concern regarding metals, and 23 regarding pesticides. Although only one participant mentioned a plasticizer (i.e., bisphenol A), we noted an underlying concern, since six professionals admitted to recommending pregnant women to somewhat avoid plastic food containers, and were aware of mother-to-child transmission and accumulation of artificial chemicals. The ubiquity of the exposure, the inability to locate the threat, and contradictory messages can all create a sense of helplessness and subsequent cognitive adjustments. Our participants also reported a lack of information, particularly on emerging pollutants. In conclusion, we found a range of valuable discourses that can aid in orienting public health strategies aimed at health professionals who have a substantial influence on their patients.
Functional Gene Clusters in Global Pathogenesis of Clear Cell Carcinoma of the Ovary Discovered by Integrated Analysis of Transcriptomes
Authors: Hsu Yueh-Han , Wang Peng-Hui , Chang Chia-Ming
Published: June 2020

Clear cell carcinoma of the ovary (ovarian clear cell carcinoma (OCCC)) is one epithelial ovarian carcinoma that is known to have a poor prognosis and a tendency for being refractory to treatment due to unclear pathogenesis. Published investigations of OCCC have mainly focused only on individual genes and lack of systematic integrated research to analyze the pathogenesis of OCCC in a genome-wide perspective. Thus, we conducted an integrated analysis using transcriptome datasets from a public domain database to determine genes that may be implicated in the pathogenesis involved in OCCC carcinogenesis. We used the data obtained from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) DataSets. We found six interactive functional gene clusters in the pathogenesis network of OCCC, including ribosomal protein, eukaryotic translation initiation factors, lactate, prostaglandin, proteasome, and insulin-like growth factor. This finding from our integrated analysis affords us a global understanding of the interactive network of OCCC pathogenesis.
Arginase-1+ Exosomes from Reprogrammed Macrophages Promote Glioblastoma Progression
Authors: Azambuja Juliana H. , Ludwig Nils , Yerneni Saigopalakrishna S. , Braganhol Elizandra , Whiteside Theresa L.
Published: June 2020

Interactions between tumor cells and tumor-associated macrophages (TAMs) are critical for glioblastoma progression. The TAMs represent up to 30% of the glioblastoma mass. The role of TAMs in tumor progression and in the mechanisms underlying tumor growth remain unclear. Using an in vitro model resembling the crosstalk between macrophages and glioblastoma cells, we show that glioblastoma-derived exosomes (GBex) reprogram M1 (mediate pro-inflammatory function) and M2 (mediate anti-inflammatory function) macrophages, converting M1 into TAMs and augmenting pro-tumor functions of M2 macrophages. In turn, these GBex-reprogrammed TAMs, produce exosomes decorated by immunosuppressive and tumor-growth promoting proteins. TAM-derived exosomes disseminate these proteins in the tumor microenvironment (TME) promoting tumor cell migration and proliferation. Mechanisms underlying the promotion of glioblastoma growth involved Arginase-1+ exosomes produced by the reprogrammed TAMs. A selective Arginase-1 inhibitor, nor-NOHA reversed growth-promoting effects of Arginase-1 carried by TAM-derived exosomes. The data suggest that GBex-reprogrammed Arginase-1+ TAMs emerge as a major source of exosomes promoting tumor growth and as a potential therapeutic target in glioblastoma.
Clinical Trials of Stem Cell Treatment for Spinal Cord Injury
Authors: Yamazaki Kazuyoshi , Kawabori Masahito , Seki Toshitaka , Houkin Kiyohiro
Published: June 2020

There are more than one million patients worldwide suffering paralysis caused by spinal cord injury (SCI). SCI causes severe socioeconomic problems not only to the patients and their caregivers but also to society; therefore, the development of innovative treatments is crucial. Many pharmacological therapies have been attempted in an effort to reduce SCI-related damage; however, no single therapy that could dramatically improve the serious long-term sequelae of SCI has emerged. Stem cell transplantation therapy, which can ameliorate damage or regenerate neurological networks, has been proposed as a promising candidate for SCI treatment, and many basic and clinical experiments using stem cells for SCI treatment have been launched, with promising results. However, the cell transplantation methods, including cell type, dose, transplantation route, and transplantation timing, vary widely between trials, and there is no consensus regarding the most effective treatment strategy. This study reviews the current knowledge on this issue, with a special focus on the clinical trials that have used stem cells for treating SCI, and highlights the problems that remain to be solved before the widespread clinical use of stem cells can be adopted.
Performance of a Three-Tier (IRT-DNA-IRT) Cystic Fibrosis Screening Algorithm in British Columbia
Authors: Sinclair Graham , McMahon Vanessa , Schellenberg Amy , Nelson Tanya N. , Chilvers Mark , Vallance Hilary
Published: June 2020

Newborn screening for Cystic Fibrosis has been implemented in most programs worldwide, but the approach used varies, including combinations of immunoreactive trypsinogen (IRT) and CFTR mutation analysis on one or more specimens. The British Columbia (BC) newborn screening program tests ~45,000 infants per year in BC and the Yukon Territory, covering almost 1.5 million km2 in western Canada. CF screening was initiated using an IRT-DNA-IRT approach with a second bloodspot card at 21 days of age for all CFTR mutation heterozygotes and any non-carriers in the top 0.1% for IRT. This second IRT was implemented to avoid sweat testing of infants without persistent hypertrypsinemia, reducing the burden of travel for families. Over nine years (2010–2018), 401,977 infants were screened and CF was confirmed in 76, and a further 28 were deemed CF screen positive inconclusive diagnosis (CFSPID). Day 21 IRT was normal in 880 CFTR mutation carriers who were quoted a very low CF risk and offered optional sweat testing. Only 13% of families opted for sweat testing and a total of 1036 sweat tests were avoided. There were six false negative CF cases (and three CFSPID) due to a low initial IRT or no CFTR mutations. Although one CFSPID case had a normal repeat IRT result, the addition of the day 21 IRT did not contribute to any CF false negatives.
The Role of Flies as Pollinators of Horticultural Crops: An Australian Case Study with Worldwide Relevance
Authors: Cook David F , Voss Sasha C , Finch Jonathan T D , Rader Romina C , Cook James M , Spurr Cameron J
Published: June 2020

Australian horticulture relies heavily on the introduced managed honey bee, Apis mellifera Linnaeus 1758 (Hymenoptera: Apidae), to pollinate crops. Given the risks associated with reliance upon a single species, it would be prudent to identify other taxa that could be managed to provide crop pollination services. We reviewed the literature relating to the distribution, efficiency and management potential of a number of flies (Diptera) known to visit pollinator-dependent crops in Australia and worldwide. Applying this information, we identified the taxa most suitable to play a greater role as managed pollinators in Australian crops. Of the taxa reviewed, flower visitation by representatives from the dipteran families Calliphoridae, Rhiniidae and Syrphidae was frequently reported in the literature. While data available are limited, there was clear evidence of pollination by these flies in a range of crops. A review of fly morphology, foraging behaviour and physiology revealed considerable potential for their development as managed pollinators, either alone or to augment honey bee services. Considering existing pollination evidence, along with the distribution, morphology, behaviour and life history traits of introduced and endemic species, 11 calliphorid, two rhiniid and seven syrphid species were identified as candidates with high potential for use in Australian managed pollination services. Research directions for the comprehensive assessment of the pollination abilities of the identified taxa to facilitate their development as a pollination service are described. This triage approach to identifying species with high potential to become significant managed pollinators at local or regional levels is clearly widely applicable to other countries and taxa.
The Circulating Fatty Acid Transporter Soluble CD36 Is Not Associated with Carotid Atherosclerosis in Subjects with Type 1 and Type 2 Diabetes Mellitus
Authors: Castelblanco Esmeralda , Sanjurjo Lucía , Barranco-Altirriba Maria , Falguera Mireia , Hernández Marta , Soldevila Berta , Sarrias Maria-Rosa , Franch-Nadal Josep , Arroyo Juan Antonio , Fernandez-Real José-Manuel , Alonso Nuria , Mauricio Didac
Published: June 2020

This study aimed to determine the association of fatty acid transporter plasma soluble cluster of differentiation 36 (sCD36) with subclinical carotid atherosclerosis (SCA). A cross-sectional study was conducted in 1023 subjects, 225 with type 1 diabetes (T1D), 276 with type 2 diabetes (T2D) and 522 who were nondiabetic. Carotid atherosclerotic plaque (CAP) presence was determined using B-mode carotid ultrasound imaging. sCD36 were analysed by ELISA, and CD36 surface receptor and mRNA expression were measured by flow cytometry and real-time PCR. Logistic regression models were used to evaluate sCD36 as a biomarker of SCA. Up to 376 (36.75%) participants had at least one CAP, 76 T1D, 164 T2D and 136 without diabetes, while the remaining 647 (63.25%) did not have any CAP. There were no differences in sCD36 between patients with and without CAP in T1D (p = 0.287) or T2D (p = 0.513). Although nondiabetic subjects with plaques had lower sCD36 levels than those without (p = 0.023), the multivariate models revealed no association of sCD36 with CAP in any of the three study groups. No differences were found in surface CD36 or CD36 mRNA expression between the patients with and without CAP. sCD36 is not associated with SCA in type 1 or type 2 diabetic or in nondiabetic subjects.
Unleashing the Diagnostic, Prognostic and Therapeutic Potential of the Neuronostatin/GPR107 System in Prostate Cancer
Authors: Sáez-Martínez Prudencio , Jiménez-Vacas Juan M. , León-González Antonio J. , Herrero-Aguayo Vicente , Montero Hidalgo Antonio J. , Gómez-Gómez Enrique , Sánchez-Sánchez Rafael , Requena-Tapia María J. , Castaño Justo P. , Gahete Manuel D. , Luque Raúl M.
Published: June 2020

Certain components of the somatostatin-system play relevant roles in Prostate Cancer (PCa), whose most aggressive phenotype (Castration-Resistant-PCa (CRPC)) remains lethal nowadays. However, neuronostatin and the G protein-coupled receptor 107 (GPR107), two novel members of the somatostatin-system, have not been explored yet in PCa. Consequently, we investigated the pathophysiological role of NST/GPR107-system in PCa. GPR107 expression was analyzed in well-characterized PCa patient′s cohorts, and functional/mechanistic assays were performed in response to GPR107-silencing and NST-treatment in PCa cells (androgen-dependent (AD: LNCaP) and androgen-independent (AI: 22Rv1/PC-3), which are cell models of hormone-sensitive and CRPC, respectively), and normal prostate cells (RWPE-1 cell-line). GPR107 was overexpressed in PCa and associated with key clinical parameters (e.g., advance stage of PCa, presence of vascular invasion and metastasis). Furthermore, GPR107-silencing inhibited proliferation/migration rates in AI-PCa-cells and altered key genes and oncogenic signaling-pathways involved in PCa aggressiveness (i.e., KI67/CDKN2D/MMP9/PRPF40A, SST5TMD4/AR-v7/In1-ghrelin/EZH2 splicing-variants and AKT-signaling). Interestingly, NST treatment inhibited proliferation/migration only in AI-PCa cells and evoked an identical molecular response than GPR107-silencing. Finally, NST decreased GPR107 expression exclusively in AI-PCa-cells, suggesting that part of the specific antitumor effects of NST could be mediated through a GPR107-downregulation. Altogether, NST/GPR107-system could represent a valuable diagnostic and prognostic tool and a promising novel therapeutic target for PCa and CRPC.
Inspiratory Muscle Training in Patients with Heart Failure
Authors: Fernandez-Rubio Hugo , Becerro-de-Bengoa-Vallejo Ricardo , Rodríguez-Sanz David , Calvo-Lobo César , Vicente-Campos Davinia , Chicharro J. L.
Published: June 2020

Background: Prior systematic reviews and meta-analysis addressed that inspiratory muscle training (IMT) improved inspiratory muscle weakness, cardiorespiratory fitness and quality of life similar to conventional exercise training as a first alternative in deconditioned patients with heart failure (HF) lead to a better adaptation to posterior exercise training. The heterogeneity and variability in a wide range of new studies about this topic led to the necessity of an updated and comprehensive narrative review. The present review aimed to analyze and update the most relevant studies about IMT in patients who suffer from HF. Methods: A narrative review was carried out about IMT in HF patients including 26 experimental studies divided into 21 clinical trials and 5 quasi-experimental studies identified through database searching in PubMed, Cochrane and PEDro. Results: There is enough evidence to state that IMT produces improvements in functional capacity of patients with HF. Nevertheless, there is not enough evidence to support that IMT could improve cardiovascular parameters, blood biomarkers or quality of life in these patients. Conclusions: Thus, IMT may be recommended to improve functional capacity in patients who suffer from HF; nevertheless, more evidence is needed regarding cardiovascular parameters, biomarkers and quality of life. Furthermore, mortality or HF hospitalization was not evaluated and most studies were not longer than 3 months. According to IMT protocols and study designs heterogeneity and mid-term follow-up, further investigations through high-quality long-term randomized clinical trials should be performed to achieve systematic reviews and meta-analysis to support strong evidence for IMT in HF patients.
Biocompatibility of Bone Marrow-Derived Mesenchymal Stem Cells in the Rat Inner Ear following Trans-Tympanic Administration
Authors: Eshraghi Adrien A. , Ocak Emre , Zhu Angela , Mittal Jeenu , Davies Camron , Shahal David , Bulut Erdogan , Sinha Rahul , Shah Viraj , Perdomo Mario M. , Mittal Rahul
Published: June 2020

Recent advancements in stem cell therapy have led to an increased interest within the auditory community in exploring the potential of mesenchymal stem cells (MSCs) in the treatment of inner ear disorders. However, the biocompatibility of MSCs with the inner ear, especially when delivered non-surgically and in the immunocompetent cochlea, is not completely understood. In this study, we determined the effect of intratympanic administration of rodent bone marrow MSCs (BM-MSCs) on the inner ear in an immunocompetent rat model. The administration of MSCs did not lead to the generation of any oxidative stress in the rat inner ear. There was no significant production of proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and IL-12, due to BM-MSCs administration into the rat cochlea. BM-MSCs do not activate caspase 3 pathway, which plays a central role in sensory cell damage. Additionally, transferase dUTP nick end labeling (TUNEL) staining determined that there was no significant cell death associated with the administration of BM-MSCs. The results of the present study suggest that trans-tympanic administration of BM-MSCs does not result in oxidative stress or inflammatory response in the immunocompetent rat cochlea.
Correction: Byun, S.-H. et al. Delayed Reconstruction of Palatomaxillary Defect Using Fibula Free Flap. J. Clin. Med. 2020, 9, 884
Authors: Byun Soo-Hwan , Lim Ho-Kyung , Yang Byoung-Eun , Kim Soung-Min , Lee Jong-Ho
Published: June 2020

The authors sincerely apologize for the imperfections made during the collection of data and wish to make the following correction to the previous paper [...]
Do We Train on Test Data? Purging CIFAR of Near-Duplicates
Authors: Barz Björn , Denzler Joachim
Published: June 2020

The CIFAR-10 and CIFAR-100 datasets are two of the most heavily benchmarked datasets in computer vision and are often used to evaluate novel methods and model architectures in the field of deep learning. However, we find that 3.3% and 10% of the images from the test sets of these datasets have duplicates in the training set. These duplicates are easily recognizable by memorization and may, hence, bias the comparison of image recognition techniques regarding their generalization capability. To eliminate this bias, we provide the “fair CIFAR” (ciFAIR) dataset, where we replaced all duplicates in the test sets with new images sampled from the same domain. The training set remains unchanged, in order not to invalidate pre-trained models. We then re-evaluate the classification performance of various popular state-of-the-art CNN architectures on these new test sets to investigate whether recent research has overfitted to memorizing data instead of learning abstract concepts. We find a significant drop in classification accuracy of between 9% and 14% relative to the original performance on the duplicate-free test set. We make both the ciFAIR dataset and pre-trained models publicly available and furthermore maintain a leaderboard for tracking the state of the art.
Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima
Authors: Nguyen Thuan Thi , Mikkelsen Maria Dalgaard , Tran Vy Ha Nguyen , Trang Vo Thi Dieu , Rhein-Knudsen Nanna , Holck Jesper , Rasin Anton B. , Cao Hang Thi Thuy , Van Tran Thi Thanh , Meyer Anne S.
Published: June 2020

Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. Fucoidans are classically extracted from brown seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction from brown seaweeds. This enzyme-assisted extraction protocol involves a one-step combined use of a commercial cellulase preparation (Cellic®CTec2) and an alginate lyase from Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal of non-fucoidan polysaccharides by Ca2+ precipitation, and ethanol-precipitation of crude fucoidan. The workability of this method is demonstrated for fucoidan extraction from Fucus distichus subsp. evanescens (basionym Fucus evanescens) and Saccharina latissima as compared with mild acidic extraction. The crude fucoidans resulting directly from the enzyme-assisted method contained considerable amounts of low molecular weight alginate, but this residual alginate was effectively removed by an additional ion-exchange chromatographic step to yield pure fucoidans (as confirmed by 1H NMR). The fucoidan yields that were obtained by the enzymatic method were comparable to the chemically extracted yields for both F. evanescens and S. latissima, but the molecular sizes of the fucoidans were significantly larger with enzyme-assisted extraction. The molecular weight distribution of the fucoidan fractions was 400 to 800 kDa for F. evanescens and 300 to 800 kDa for S. latissima, whereas the molecular weights of the corresponding chemically extracted fucoidans from these seaweeds were 10–100 kDa and 50–100 kDa, respectively. Enzyme-assisted extraction represents a new gentle strategy for fucoidan extraction and it provides new opportunities for obtaining high yields of native fucoidan structures from brown macroalgae.
Electrochemical Approach for Isolation of Chitin from the Skeleton of the Black Coral Cirrhipathes sp. (Antipatharia)
Authors: Nowacki Krzysztof , Stępniak Izabela , Langer Enrico , Tsurkan Mikhail , Wysokowski Marcin , Petrenko Iaroslav , Khrunyk Yuliya , Fursov Andriy , Bo Marzia , Bavestrello Giorgio , Joseph Yvonne , Ehrlich Hermann
Published: June 2020

The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks and sponges, there are only a few reports concerning its isolation from corals, and especially black corals (Antipatharia). In this work, we report the stepwise isolation and identification of chitin from Cirrhipathes sp. (Antipatharia, Antipathidae) for the first time. The proposed method, aiming at the extraction of the chitinous scaffold from the skeleton of black coral species, combined a well-known chemical treatment with in situ electrolysis, using a concentrated Na2SO4 aqueous solution as the electrolyte. This novel method allows the isolation of α-chitin in the form of a microporous membrane-like material. Moreover, the extracted chitinous scaffold, with a well-preserved, unique pore distribution, has been extracted in an astoundingly short time (12 h) compared to the earlier reported attempts at chitin isolation from Antipatharia corals.
Effects of Travel Speed on the Microstructure and Abrasion Resistance of Hardfacing Alloys Deposited with Composite Powder Particles and Solid Wire
Authors: Yao Huiwen , Gong Jianxun , Cheng Shiyao , Liu Chao , Huang Hongjiang
Published: June 2020

Composite powder particles (CPP) preset on base metals were fused with a solid wire to form a melt by the intense heat provided by the arc. An X-ray diffractometer, scanning electron microscopy, and energy dispersive spectrometer were employed to investigate the effects of travel speed on the microstructure and abrasion resistance. It was found that the microstructure of hardfacing alloys with CPP consists of γ-Fe, M7C3, and (Ti, V) C. With an increase in the travel speed from 3.5 to 6 mm/s, the microstructure with CPP changed from a hypoeutectic to hypereutectic structure. For hardfacing alloys with CPP, the increase in the travel speed not only contributed to a reduction of the dilution ratio of base metals, but also deliberately increased the volume fraction of primary M7C3-type carbides, which indicated that the bonding function executed on powder components led to a significant improvement in abrasion resistance and increased the utilization ratio of the alloying elements. The wear mechanism of hardfacing alloys included micro-cutting of abrasive particles and micro-spalling.
In Silico Evaluation of the Effectivity of Approved Protease Inhibitors against the Main Protease of the Novel SARS-CoV-2 Virus
Authors: Eleftheriou Phaedra , Amanatidou Dionysia , Petrou Anthi , Geronikaki Athina
Published: June 2020

The coronavirus disease, COVID-19, caused by the novel coronavirus SARS-CoV-2, which first emerged in Wuhan, China and was made known to the World in December 2019 turned into a pandemic causing more than 126,124 deaths worldwide up to April 16th, 2020. It has 79.5% sequence identity with SARS-CoV-1 and the same strategy for host cell invasion through the ACE-2 surface protein. Since the development of novel drugs is a long-lasting process, researchers look for effective substances among drugs already approved or developed for other purposes. The 3D structure of the SARS-CoV-2 main protease was compared with the 3D structures of seven proteases, which are drug targets, and docking analysis to the SARS-CoV-2 protease structure of thirty four approved and on-trial protease inhibitors was performed. Increased 3D structural similarity between the SARS-CoV-2 main protease, the HCV protease and α-thrombin was found. According to docking analysis the most promising results were found for HCV protease, DPP-4, α-thrombin and coagulation Factor Xa known inhibitors, with several of them exhibiting estimated free binding energy lower than −8.00 kcal/mol and better prediction results than reference compounds. Since some of the compounds are well-tolerated drugs, the promising in silico results may warrant further evaluation for viral anticipation. DPP-4 inhibitors with anti-viral action may be more useful for infected patients with diabetes, while anti-coagulant treatment is proposed in severe SARS-CoV-2 induced pneumonia.
Unveiling the Lewis Acid Catalyzed Diels–Alder Reactions Through the Molecular Electron Density Theory
Authors: Domingo Luis R. , Ríos-Gutiérrez Mar , Pérez Patricia
Published: June 2020

The effects of metal-based Lewis acid (LA) catalysts on the reaction rate and regioselectivity in polar Diels–Alder (P-DA) reactions has been analyzed within the molecular electron density theory (MEDT). A clear linear correlation between the reduction of the activation energies and the increase of the polar character of the reactions measured by analysis of the global electron density transfer at the corresponding transition state structures (TS) is found, a behavior easily predictable by analysis of the electrophilicity ω and nucleophilicity N indices of the reagents. The presence of a strong electron-releasing group in the diene changes the mechanism of these P-DA reactions from a two-stage one-step to a two-step one via formation of a zwitterionic intermediate. However, this change in the reaction mechanism does not have any chemical relevance. This MEDT study makes it possible to establish that the more favorable nucleophilic/electrophilic interactions taking place at the TSs of LA catalyzed P-DA reactions are responsible for the high acceleration and complete regioselectivity experimentally observed.
Synthetic (E)-3-Phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium Chloride Derivatives as Promising Chemotherapy Agents on Cell Lines Infected with HTLV-1
Authors: Sousa-Pereira Danilo , Silva de Oliveira Thais , Paiva Rojane O. , Chaves Otávio Augusto , Netto-Ferreira José C. , Echevarria-Lima Juliana , Echevarria Aurea
Published: June 2020

Synthesis of four compounds belonging to mesoionic class, (E)-3-phenyl-5-(phenylamino)-2-styryl-1,3,4-thiadiazol-3-ium chloride derivatives (5a–d) and their biological evaluation against MT2 and C92 cell lines infected with human T-cell lymphotropic virus type-1 (HTLV-1), which causes adult T-cell leukemia/lymphoma (ATLL), and non-infected cell lines (Jurkat) are reported. The compounds were obtained by convergent synthesis under microwave irradiation and the cytotoxicity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Results showed IC50 values of all compounds in the range of 1.51–7.70 μM in HTLV-1-infected and non-infected cells. Furthermore, it was observed that 5b could induce necrosis after 24 h for Jurkat and MT2 cell lines. The experimental (fluorimetric method) and theoretical (molecular docking) results suggested that the mechanism of action for 5b could be related to its capacity to intercalate into DNA. Moreover, the preliminary pharmacokinetic profile of the studied compounds (5a–d) was obtained through human serum albumin (HSA) binding affinity using multiple spectroscopic techniques (circular dichroism, steady-state and time-resolved fluorescence), zeta potential and molecular docking calculations. The interaction HSA:5a–d is spontaneous and moderate (Ka ~ 104 M−1) via a ground-state association, without significantly perturbing both the secondary and surface structures of the albumin in the subdomain IIA (site I), indicating feasible biodistribution in the human bloodstream.
SIRT1 Activation by Equisetum arvense L. (Horsetail) Modulates Insulin Sensitivity in Streptozotocin Induced Diabetic Rats
Authors: Hegedűs Csaba , Muresan Mariana , Badale Andrea , Bombicz Mariann , Varga Balázs , Szilágyi Anna , Sinka Dávid , Bácskay Ildikó , Popoviciu Mihaela , Magyar Ioan , Szarvas Mária Magdolna , Szőllősi Erzsébet , Németh József , Szilvássy Zoltán , Pallag Annamaria , Kiss Rita
Published: June 2020

BACKGROUND: Equisetum arvense L., commonly known as field horsetail is a perennial fern of which extracts are rich sources of phenolic compounds, flavonoids, and phenolic acids. Activation of SIRT1 that was shown to be involved in well-known signal pathways of diabetic cardiomyopathy has a protective effect against oxidative stress, inflammatory processes, and apoptosis that are the basis of diseases such as obesity, diabetes mellitus, or cardiovascular diseases. The aim of our study was to evaluate the antidiabetic and cardioprotective effects of horsetail extract in streptozotocin induced diabetic rats. METHODS: Diabetes was induced by a single intraperitoneal injection of 45 mg/kg streptozotocin. In the control groups (healthy and diabetic), rats were administered with vehicle, whilst in the treated groups, animals were administered with 50, 100, or 200 mg/kg horsetail extract, respectively, for six weeks. Blood glucose levels, glucose tolerance, and insulin sensitivity were determined, and SIRT1 levels were measured from the cardiac muscle. RESULTS: The horsetail extract showed moderate beneficial changes in blood glucose levels and exhibited a tendency to elevate SIRT1 levels in cardiomyocytes, furthermore a 100 mg/kg dose also improved insulin sensitivity. CONCLUSIONS: Altogether our results suggest that horsetail extract might have potential in ameliorating manifested cardiomyopathy acting on SIRT1.
Extraction and Physicochemical Characterization of Chitin from Cicada orni Sloughs of the South-Eastern French Mediterranean Basin
Authors: Poerio Aurelia , Petit Chloé , Jehl Jean-Philippe , Arab-Tehrany Elmira , Mano João F. , Cleymand Franck
Published: June 2020

Chitin is a structural polysaccharide of the cell walls of fungi and exoskeletons of insects and crustaceans. In this study, chitin was extracted, for the first time in our knowledge, from the Cicada orni sloughs of the south-eastern French Mediterranean basin by treatment with 1 M HCl for demineralization, 1 M NaOH for deproteinization, and 1% NaClO for decolorization. The different steps of extraction were investigated by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM). Results demonstrated that the extraction process was efficiently performed and that Cicada orni sloughs of the south-eastern French Mediterranean basin have a high content of chitin (42.8%) in the α-form with a high degree of acetylation of 96% ± 3.4%. These results make Cicada orni of the south-eastern French Mediterranean basin a new and promising source of chitin. Furthermore, we showed that each step of the extraction present specific characteristics (for example FTIR and XRD spectra and, consequently, distinct absorbance peaks and values of crystallinity as well as defined values of maximum degradation temperatures identifiable by TGA analysis) that could be used to verify the effectiveness of the treatments, and could be favorably compared with other natural chitin sources.