bluuebunny/crossref_metadata_2025 huggingface dataset:
Nutritional Characterization of Strychnos madagascariensis Fruit Flour Produced by Mozambican Communities and Evaluation of Its Contribution to Nutrient Adequacy
Authors: Chemane Sandra S. I. , Ribeiro Mafalda , Pinto Edgar , Pinho Susana C. M. , Martins Zita Sá , Almeida Agostinho , Ferreira Isabel M. P. L. V. O. , Khan Maida , Pinho Olívia , Casal Susana , Viegas Olga
Published: February 2022

The indigenous fruit Strychnos madagascariensis is usually processed to flour, called nfuma, being highly consumed during staple food shortage. This study aimed to evaluate the nutritional composition of nfuma and its nutrient adequacy. Flours from four districts of Mozambique were analyzed using AOAC methods for proximate composition, HPLC for sugar, amino acids (AA), vitamin E and carotenoids and ICP-MS and FAAS for minerals. The results showed that nfuma stands out for its high content of fat (26.3–27.8%), mainly oleic acid, fiber (>6%), vitamin E (6.7 to 8.0 mg/100 g) and carotenes (2.2 to 2.6 mg/100 g). The main amino acids of nfuma protein were Arg, Asp and Glu, and Lys was the limiting one. The mineral composition reveals K (~1200 to 1700 mg/100 g) as the main macromineral followed by Mg > Ca > Na. The main trace element was Mn (~4 mg/100 g) followed by Fe > Zn > Cu > Cr > Co. Aluminum (~3 mg/100 g) was the main non-essential element and Rb, Ni, Sr, Ba, V, Cd were also quantified. Assuming the daily consumption of 50 g, nfuma provides 82% of Vitamin A dietary reference value for toddlers, while the consumption of 100 g contributes to 132% and 60% of Mn and vitamin A DRV for adults, respectively. Despite the nutritional advantages of nfuma, this flour can be a source of Ni, highlighting the importance of the study of good practices in its preparation to decrease the exposure to non-essential elements.
The Dual Nature of Amaranth—Functional Food and Potential Medicine
Authors: Baraniak Justyna , Kania-Dobrowolska Małgorzata
Published: February 2022

The beneficial health-promoting properties of plants have been known to mankind for generations. Preparations from them are used to create recipes for dietary supplements, functional food, and medicinal products. Recently, amaranth has become an area of increasing scientific and industrial interest. This is due to its valuable biological properties, rich phytochemical composition, and wide pharmacological activity. Amaranth is a pseudo-cereal crop with a dual character, combining the features of food and health-promoting product. This paper briefly and concisely reviews the current information on the chemical composition of amaranth, the value of its supplementation, the status of amaranth as a food ingredient as well as its key biological and pharmacological activities. The beneficial biological properties of amaranth preparations described in this paper may be an incentive to conduct further in-depth scientific research in this field and also to promote the development of innovative technologies in the food and cosmetics industry with the use of this plant.
Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells
Authors: Chen Yijian , Jiang Yuyan , Lao Jihui , Zhou Yankuan , Su Lida , Huang Xiao
Published: February 2022

FAM49B/Fam49b is a member of the Fam49 (Family with sequence similarity 49) gene family, which is characterized by the conserved domain, DUF1394 (Domain of Unknown Function 1394). It has also been named CYRI-B (CYFIP related RAC1 interactor B), implicating its important function of regulating RAC1-driven cytoskeleton remolding. In this study, to further investigate its functions and mechanisms affecting cell behaviors, HEK293T cells (where FAM49B is highly expressed) were used to establish a FAM49B knockout cell line by CRISPR/Cas9 genome editing technology. Our data have clearly revealed that there are triple alleles of FAM49B in the genome of HEK293T cells. Meanwhile, the proliferation deficiency of the FAM49B KO HEK293T cell line and the significantly changed cell proliferation related gene expression profiles, such as CCND1, have been uncovered. At the same time, the existence of isoform 3 has been confirmed in HEK293T cells. Our studies have suggested that FAM49B may also affect cell proliferation via Cyclins, besides its influence on the cytoskeleton.
Intraplate Strike-Slip Corridor within South America (NE Border of the Paraná Basin) Unveiled by Structural Analysis of Faults and Fracture Swarms
Authors: Cianfarra Paola , Pinheiro Marcos Roberto , Villela Fernando Nadal Junqueira , Salvini Francesco
Published: February 2022

We present the effect of neotectonics in intracratonic settings as revealed by the surface, brittle deformation associated to a regionally-sized shear corridor, which affects Southeastern Brazil. The deformation zone is characterized by the presence of nearly orthogonal fracture sets, interpreted as systematic and non-systematic joints often cutting Quaternary deposits. An original methodology of fault and joint inversion by the Monte Carlo converging approach is used to infer multiple paleostress fields. The method provides the best orientation of the principal paleo-stresses responsible for the observed fracturing. At each step of the inversion process, structures are uniquely associated to the stress tensor that provides the lowest error. The results showed the poly-phased tectonic history of the shear corridor studied and paleostresses compatible with a regional strike-slip motion. Specifically, an E-W, left-lateral shear was followed by an E-W, right-lateral kinematics related to the post-Paleogene drifting of South American Plate and its clockwise rotation. The latter tectonic event is presently responsible for brittle deformation observed in Quaternary deposits. The proposed deformation corridor may represent the Cenozoic reactivation of an ancient weakness zone. We speculate that the described intraplate strike-slip deformation belt represents the continental prosecution of the Rio de Janeiro fracture zone.
Energy Demand in Surface Soils for Earthquake Engineering by Vertical Array Strong Motion Records
Authors: Kokusho Takaji
Published: February 2022

In earthquake engineering, acceleration has played a major role, while wave energy has rarely been considered as a demand in design. In order to understand earthquake damage mechanism in terms of energy, the demand in terms of wave energy in surface soil layers is studied here, assuming one-dimensional SH wave propagation by using a number of vertical array records during nine strong earthquakes in Japan. A clear decreasing trend of the energy demand with decreasing ground depth and decreasing surface soil stiffness has been found as well as a propensity of incident energies calculated at bedrocks being roughly compatible with empirical formulas. How the energy demand is correlated with structural damage is also discussed in simplified models to show that induced structural strain is governed by upward energy flux, degree of structural resonance, and impedance ratio between structure and ground and structural stiffness. In low-damping brittle superstructures, wave energy flux in resonance and associated predominant frequency are decisive in determining the damage, while cumulative wave energy determines the damage in high damping ductile soil and massive concrete structures. The trend of lower energy demand in softer soil sites may not be contradictory, with a widely accepted perception that softer soil sites tend to suffer heavier earthquake damage as far as geotechnical damage is concerned.
Oral Hygiene Practices and Oral Health Knowledge among Students in Split, Croatia
Authors: Tadin Antonija , Poljak Guberina Renata , Domazet Josipa , Gavic Lidia
Published: February 2022

Background: Knowledge of oral health is a fundamental prerequisite for healthy behavior, allowing individuals to take measures to protect their overall health. This cross-sectional study aimed to examine the knowledge of oral health as well as to assess the oral hygiene habits among healthcare and non-healthcare students. Methods: The study was based on a questionnaire and was conducted among 1088 students. Most of the students, 67.6% were non-healthcare students. Data were processed by Mann–Whitney or Kruskal–Wallis one-way ANOVA test. Results: The correct median score and interquartile range were 11 (9–13) for all surveyed students, 11 (9–12) for students in non-healthcare programs and 13 (11–14) for students in healthcare programs. Students did not significantly differ in the knowledge of oral health by gender (p = 0.082) but did differ by age, study program, and year of study (p ≤ 0.001). Students whose family members work in the field of dental medicine also showed better oral health knowledge (p ≤ 0.001). Conclusion: The results showed good oral health knowledge among tested university students. However, it is important to emphasize that students who showed better knowledge more often used additional aids to maintain oral hygiene; therefore, the obtained data underline the importance of students’ further education in order to better understand and maintain oral health.
Research on the Pathway of Green Financial System to Implement the Realization of China’s Carbon Neutrality Target
Authors: Wang Gaoweijia , Li Shanshan , Yang Li
Published: February 2022

To answer to global climate change, promote climate governance and map out a grand blueprint for sustainable development, carbon neutrality has become the target and vision of all countries. Green finance is a means to coordinate economic development and environmental governance. This paper mainly studies the trend of carbon emission reduction in China in the next 40 years under the influence of green finance development and how to develop and improve China’s green finance system to help China achieve the goal of “carbon neutrality by 2060”. The research process and conclusions are as follows: (1) Through correlation test and data analysis, it is concluded that the development of green finance is an important driving force to achieve carbon neutrality. (2) The grey prediction GM (1,1) model is used to forecast the data of carbon dioxide emissions, green credit balance, green bond issuance scale and green project investment in China from 2020 to 2060. The results show that they will all increase year by year in the next 40 years. (3) BP neural network model is used to further predict carbon dioxide emissions from 2020 to 2060. It is expected that China’s CO2 emissions will show an “inverted V” trend in the next 40 years, and China is expected to achieve a carbon peak in 2032 and be carbon neutral in 2063. Based on the results of the research above, this paper provides a supported path of implementing the realization of the carbon-neutral target of China from the perspective of developing and improving green financial system, aiming to provide references for China to realize the vision of carbon neutrality, providing policy suggestions for relevant departments, and provide ideas for other countries to accelerate the realization of carbon neutrality.
Role of Body Mass and Physical Activity in Autonomic Function Modulation on Post-COVID-19 Condition: An Observational Subanalysis of Fit-COVID Study
Authors: Freire Ana Paula Coelho Figueira , Lira Fabio Santos , Morano Ana Elisa von Ah , Pereira Telmo , Coelho-E-Silva Manuel-João , Caseiro Armando , Christofaro Diego Giulliano Destro , Marchioto Júnior Osmar , Dorneles Gilson Pires , Minuzzi Luciele Guerra , Pinho Ricardo Aurino , Silva Bruna Spolador de Alencar
Published: February 2022

The harmful effects of coronavirus disease 2019 (COVID-19) can reach the autonomic nervous system (ANS) and endothelial function. Therefore, the detrimental multiorgan effects of COVID-19 could be induced by deregulations in ANS that may persist after the acute SARS-CoV-2 infection. Additionally, investigating the differences in ANS response in overweight/obese, and physically inactive participants who had COVID-19 compared to those who did not have the disease is necessary. The aim of the study was to analyze the autonomic function of young adults after mild-to-moderate infection with SARS-CoV-2 and to assess whether body mass index (BMI) and levels of physical activity modulates autonomic function in participants with and without COVID-19. Patients previously infected with SARS-CoV-2 and healthy controls were recruited for this cross-sectional observational study. A general anamnesis was taken, and BMI and physical activity levels were assessed. The ANS was evaluated through heart rate variability. A total of 57 subjects were evaluated. Sympathetic nervous system activity in the post-COVID-19 group was increased (stress index; p = 0.0273). They also presented lower values of parasympathetic activity (p < 0.05). Overweight/obese subjects in the post-COVID-19 group presented significantly lower parasympathetic activity and reduced global variability compared to non-obese in control group (p < 0.05). Physically inactive subjects in the post-COVID-19 group presented significantly higher sympathetic activity than active subjects in the control group. Parasympathetic activity was significantly increased in physically active subjects in the control group compared to the physically inactive post-COVID-19 group (p < 0.05). COVID-19 promotes changes in the ANS of young adults, and these changes are modulated by overweight/obesity and physical activity levels.
A Journey towards Resilience: Coping Strategies Adopted by Parents with Children Having Autism Spectrum Disorder in Northeast Malaysia
Authors: Yaacob Wan Natrah Wan , Yaacob Lili Husniati , Zulkifli Maryam Mohd , Muhamad Rosediani
Published: February 2022

Background: The prevalence of Autism Spectrum Disorder (ASD) has been increasing for the past two decades. Parents with autism have been known to be at risk of psychological distress and maladaptation, but many parents were able to overcome this adversity and lead to a good quality of life. Methods: In-depth interviews were conducted among 21 parents of children with ASD. Interviews were transcribed and evaluated using thematic analysis. Results: The analysis discovered three main themes: acceptance and positive outlook, reaching for helping hands, and understanding autism and finding its solutions. Conclusions: Although the findings cannot be generalised to other populations with ASD, this study provides a detailed perspective on their unique coping strategies. Insights gained from this study could help health care providers, authorities, and communities to address a specific need and able to advocate relevant support measures to assist them.
Identifying Types of Eco-Anxiety, Eco-Guilt, Eco-Grief, and Eco-Coping in a Climate-Sensitive Population: A Qualitative Study
Authors: Ágoston Csilla , Csaba Benedek , Nagy Bence , Kőváry Zoltán , Dúll Andrea , Rácz József , Demetrovics Zsolt
Published: February 2022

Background: Climate change is one of the greatest challenges of the 21st century and it can affect mental health either directly through the experience of environmental traumas or indirectly through the experience of emotional distress and anxiety about the future. However, it is not clear what possible subtypes of the emerging “psychoterratic” syndromes such as eco-anxiety, eco-guilt, and eco-grief exist, how much distress they may cause, and to what extent they facilitate ecofriendly behavior. Methods: We analyzed semi-structured interviews (N = 17) focusing on the thoughts, emotions, and behaviors related to climate change by using a combination of inductive and deductive qualitative methods. Results and conclusions: The interviews revealed six eco-anxiety components, eight types of eco-guilt, and two types of eco-grief that help to understand the multifactorial nature of these phenomena. The six categories of coping strategies are in line with traditional coping models, and they are linked in various ways to pro-environmental behavior and the management of negative emotions. The results can help practitioners to gain a deeper understanding of emotions related to climate change and how to cope with them, and researchers to develop comprehensive measurement tools to assess these emotions.
Effect of Urbanization on the River Network Structure in Zhengzhou City, China
Authors: Wang Hongxiang , Huang Lintong , Hu Jianwen , Yang Huan , Guo Wenxian
Published: February 2022

Hydrological problems, such as flood disasters, can be caused by the influence of urbanization on river network structures in plain areas. Taking the main urban region of Zhengzhou city as the research area, based on six remote sensing images from 1992 to 2015, the modified normalized difference water index method and a land-use transfer matrix were used to reconstruct river network data to study the temporal and spatial changes in the river system. In addition, the analytic hierarchy process and the entropy weight method were used to construct pattern indexes of the river system to quantitatively evaluate the inner relationship between the urbanization process and the river network structure in the plain area. The results showed that the percentages of arable land, forest and grassland, water, and unused land in Zhengzhou that was transferred to construction land from 1992 to 2015 were 59.10%, 51.05%, 29.83%, and 58.76%, respectively. In the past 34 years, the morphological indices, structural indices, and connectivity indices of the river system experienced a trend of high to low, and then increased, with the structural indices being significantly correlated with construction land use (p < 0.05). The regression equation R2 between urbanization level and river length, water area, river network density, water surface rate, connection rate, and connectivity ranged from 0.677 to 0.966, which could well reflect the response relationship between urbanization and the river network. In addition, the outflow was greater than the inflow, which has destroyed the natural structure of the channel.
Quantitative Electroencephalography (QEEG) as an Innovative Diagnostic Tool in Mental Disorders
Authors: Kopańska Marta , Ochojska Danuta , Dejnowicz-Velitchkov Agnieszka , Banaś-Ząbczyk Agnieszka
Published: February 2022

Quantitative electroencephalography (QEEG) is becoming an increasingly common method of diagnosing neurological disorders and, following the recommendations of The American Academy of Neurology (AAN) and the American Clinical Neurophysiology Society (ACNS), it can be used as a complementary method in the diagnosis of epilepsy, vascular diseases, dementia, and encephalopathy. However, few studies are confirming the importance of QEEG in the diagnosis of mental disorders and changes occurring as a result of therapy; hence, there is a need for analyses in this area. The aim of the study is analysis of the usefulness of QEEG in the diagnosis of people with generalized anxiety disorders. Our research takes the form of case studies. The paper presents an in-depth analysis of the QEEG results of five recently studied people with a psychiatric diagnosis: generalized anxiety disorder. The results show specific pattern amplitudes at C3 and C4. In all of the examined patients, two dependencies are repeated: low contribution of the sensorimotor rhythm (SMR) wave amplitudes and high beta2 wave amplitudes, higher or equal to the alpha amplitudes. The QEEG study provides important information about the specificity of brain waves of people with generalized anxiety disorder; therefore, it enables the preliminary and quick diagnosis of dysfunction. It is also possible to monitor changes due to QEEG, occurring as a result of psychotherapy, pharmacological therapy and EEG-biofeedback.
Optimizing the Water Ecological Environment of Mining Cities in the Yangtze River Economic Belt Using the Cloud Model, CV-TOPSIS, and Coupling Coordination Degree
Authors: Wang Ran , Lin Hao , Cheng Jinhua , Xu Zixi , Feng Haoying , Tang Yameng
Published: February 2022

The Yangtze River Economic Belt (YREB) is the core region for the security of mineral resources in China and is a strategic water source containing rich water resources. Coordinating the security of mineral resources and water resources in the YREB is a key problem. Establishing and optimizing the water ecological environment (WEE) is crucial for addressing this problem in mining cities, which are the main bases for the supply of mineral resources. This study applies the cloud model, CV-TOPSIS, the standard deviation ellipse, and the coupling coordination degree model to evaluate the WEE and the coordinated development state, and to optimize the WEE. The results show that: (1) the WEE of mining cities in the YREB is generally good; (2) the protection of WEE in most mining cities has achieved significant results recently, and the results in the downstream are more remarkable than those in the mid-upstream; (3) the coordinated development of WEE in regenerative mining cities is better than that of mature and declining cities; and (4) most mining cities still belong to the lagging type of water environment (heavy metal pollution has been better treated and the threat of water ecological security caused by heavy metal pollution is low). This study suggests improvements to the sewer system, promotes WEE management in the mid-upstream, and propels the transformational development of mature and declining mining cities in advance.
How to Educate the Public about Dental Trauma—A Scoping Review
Authors: Nowosielska Magdalena , Bagińska Joanna , Kobus Agnieszka , Kierklo Anna
Published: February 2022

Educating the general population about dental trauma is of public health interest. The aim of this scoping review was to map research on traumatic dental injuries (TDIs) education in the general population and to identify the most relevant methods of knowledge transfer. PubMed, Scopus, Web of Science All Databases, reference lists, and grey literature were searched. Articles in English published between 2000 and 2020 were included. A total of 32 articles fulfilled inclusion criteria. The most frequently tested modality was lecture/seminar/workshop. Studies focused mainly on teachers and medical staff as target groups. Post-intervention evaluation showed an increase in knowledge. In long-term follow-up, a decrease in knowledge was found. The effectiveness of different modalities varied. Studies comparing single-modal and multimodal approaches did not confirm the effect of combined methods. Printed materials are a practical mode for laypeople. Lectures should be reserved for professions with high probability of coming into contact with a TDI victim. The Internet can be a promising tool to educate people. Educators have to choose the method of communication most appropriate for the target population. The education should include topics related to dental trauma prevention. Further research is needed to investigate the effectiveness of multimodal TDI education.
Spatiotemporal Differentiation and Driving Force Analysis of the High-Quality Development of Urban Agglomerations along the Yellow River Basin
Authors: Chen Yu , Miao Qianqian , Zhou Qian
Published: February 2022

The ecological protection and high-quality development (HQD) of the Yellow River Basin (YRB) have been promoted as national strategies. An urban agglomeration is the basic unit of the YRB used to participate in international competitions. Taking seven urban agglomerations covering 70 cities along the YRB as the sample, this paper establishes a high-quality evaluation system and uses the entropy method and exploratory spatial data analysis (ESDA) to analyze the HQD levels of the seven urban agglomerations along the YRB from 2009 to 2018. In addition, geographically-weighted regression (GWR) is adopted to analyze the influencing factors. The results show that: (1) the gap in the HQD of the seven urban agglomerations gradually narrows, showing a spatial pattern of “high in the east, low in the west, and depression in the middle”; (2) the HQD levels of the seven urban agglomerations have a strong spatial correlation, and the patterns of cold and hot spots have not changed substantially, showing the spatial distribution of “hot in the east, cold in the west”; (3) the degree of influence of each driving factor on the HQD differs among the seven urban agglomerations. The order is as follows: industrial structure upgrading index > proportion of R&D expenditure > urbanization rate > internet penetration rate > proportion of urban construction area > proportion of days reaching the air standard. These findings show that advanced industrial structure and technology are the two core driving forces for the HQD of the urban agglomerations along the YRB.
Pain Evaluation in the Paediatric Emergency Department: Differences in Ratings by Patients, Parents and Nurses
Authors: Rybojad Beata , Sieniawski Daniel , Rybojad Paweł , Samardakiewicz Marzena , Aftyka Anna
Published: February 2022

The pain experienced by paediatric patients is rarely evaluated in emergency departments. The aim of the present study was to compare the degree of conformity in patients’ pain severity when assessed by themselves (if possible), their parents and a triage nurse trained in pain evaluation. Methods: A cross-sectional observational study was conducted at a tertiary paediatric emergency department in Eastern Poland involving children (aged six months to eighteen years), their parents and nurses. The patients had their pain assessed while collecting a medical history. For children ≥ four years of age, the Numerical Rate Scale was used by patients, parents and nurses to evaluate pain. Patients under four years of age were evaluated by parents and nurses using the FLACC scale. Results: Eighty patients and their parents were enrolled in the study. For children ≥ four years, patients rated their pain significantly higher than both their parents (p = 0.03) and nurses (p < 0.001), with the latter group producing the lowest scores. For children under four years of age, parental pain assessments did not significantly differ from those of nurses. Conclusion: Compared to the patients themselves and their parents, nurses tended to assign lower pain scores for children. Pain should be assessed on admission to the ED and, whenever possible, by the patients themselves.
The Roles of NOD-like Receptors in Innate Immunity in Otitis Media
Authors: You Myung-Won , Kim Dokyoung , Lee Eun-Hye , Park Dong-Choon , Lee Jae-Min , Kang Dae-Woong , Kim Sang-Hoon , Yeo Seung-Geun
Published: February 2022

Acute otitis media (AOM) can persist or lead to various complications in individuals in which the innate immune system is impaired. In this context, impaired expression of nucleotide-binding oligomerization domain (NOD)-like receptor (NLR), an intracellular pathogen-recognition receptor (PRR), is involved in the etiology of OM in humans and animals, affecting its development, severity, chronicity, recurrence, and associated complications. To assess this relationship, we reviewed literature reports relating NLR expression patterns with the pathophysiology and clinical features of OM in the larger context of impaired innate immunity. We summarized the results of published studies on the expression of NLRs in animals and humans in acute otitis media (AOM), otitis media with effusion (OME), chronic otitis media (COM) with cholesteatoma, and COM without cholesteatoma. NLRs were expressed mainly in association with bacterial infection in AOM, OME, COM with cholesteatoma, and COM without cholesteatoma. In addition, expression of NLRs was affected by the presence or absence of bacteria, fluid characteristics, disease recurrence, tissue type, and repeated surgery. Various factors of the innate immune system are involved in the pathogenesis of OM in the middle ear. NLRs are expressed in AOM, OME, COM with cholesteatoma, and COM without cholesteatoma. Impaired NLR expression induced the development, chronicity and recurrence of OM and exacerbated associated complications, indicating that NLRs have important roles in the pathogenesis of OM.
Genome-Wide Identification and Analysis of bZIP Gene Family and Resistance of TaABI5 (TabZIP96) under Freezing Stress in Wheat (Triticum aestivum)
Authors: Liang Yi , Xia Jingqiu , Jiang Yunshuang , Bao Yuzhuo , Chen Huichan , Wang Duojia , Zhang Da , Yu Jing , Cang Jing
Published: February 2022

The basic leucine zipper (bZIP) regulates plant growth and responds to stress as a key transcription factor of the Abscisic acid (ABA) signaling pathway. In this study, TabZIP genes were identified in wheat and the gene structure, physicochemical properties, cis-acting elements, and gene collinearity were analyzed. RNA-Seq and qRT-PCR analysis showed that ABA and abiotic stress induced most TabZIP genes expression. The ectopic expression of TaABI5 up-regulated the expression of several cold-responsive genes in Arabidopsis. Physiological indexes of seedlings of different lines under freezing stress showed that TaABI5 enhanced the freezing tolerance of plants. Subcellular localization showed that TaABI5 is localized in the nucleus. Furthermore, TaABI5 physically interacted with cold-resistant transcription factor TaICE1 in yeast two-hybrid system. In conclusion, this study identified and analyzed members of the TabZIP gene family in wheat. It proved for the first time that the gene TaABI5 affected the cold tolerance of transgenic plants and was convenient for us to understand the cold resistance molecular mechanism of TaABI5. These results will provide a new inspiration for further study on improving plant abiotic stress resistance.
Thermochemical Characterization of Eight Seaweed Species and Evaluation of Their Potential Use as an Alternative for Biofuel Production and Source of Bioactive Compounds
Authors: Cassani Lucía , Lourenço-Lopes Catarina , Barral-Martinez Marta , Chamorro Franklin , Garcia-Perez Pascual , Simal-Gandara Jesus , Prieto Miguel A.
Published: February 2022

Algae are underexplored resources in Western countries and novel approaches are needed to boost their industrial exploitation. In this work, eight edible seaweeds were subjected to their valorization in terms of nutritional characterization, thermochemical properties, and bioactive profile. Our results suggest that seaweeds present a rich nutritional profile, in which carbohydrates are present in high proportions, followed by a moderate protein composition and a valuable content of ω-3 polyunsaturated fatty acids. The thermochemical characterization of seaweeds showed that some macroalgae present a low ash content and high volatile matter and carbon fixation rates, being promising sources for alternative biofuel production. The bioactive profile of seaweeds was obtained from their phenolic and carotenoid content, together with the evaluation of their associated bioactivities. Among all the species analyzed, Porphyra purpurea presented a balanced composition in terms of carbohydrates and proteins and the best thermochemical profile. This species also showed moderate anti-inflammatory activity. Additionally, Himanthalia elongata extracts showed the highest contents of total phenolics and a moderate carotenoid content, which led to the highest rates of antioxidant activity. Overall, these results suggest that seaweeds can be used as food or functional ingredient to increase the nutritional quality of food formulations.
Enzymes-Assisted Extraction of Plants for Sustainable and Functional Applications
Authors: Streimikyte Paulina , Viskelis Pranas , Viskelis Jonas
Published: February 2022

The scientific community and industrial companies have discovered significant enzyme applications to plant material. This rise imparts to changing consumers’ demands while searching for ‘clean label’ food products, boosting the immune system, uprising resistance to bacterial and fungal diseases, and climate change challenges. First, enzymes were used for enhancing production yield with mild and not hazardous applications. However, enzyme specificity, activity, plant origin and characteristics, ratio, and extraction conditions differ depending on the goal. As a result, researchers have gained interest in enzymes’ ability to cleave specific bonds of macroelements and release bioactive compounds by enhancing value and creating novel derivatives in plant extracts. The extract is enriched with reducing sugars, phenolic content, and peptides by disrupting lignocellulose and releasing compounds from the cell wall and cytosolic. Nonetheless, depolymerizing carbohydrates and using specific enzymes form and release various saccharides lengths. The latest studies show that oligosaccharides released and formed by enzymes have a high potential to be slowly digestible starches (SDS) and possibly be labeled as prebiotics. Additionally, they excel in new technological, organoleptic, and physicochemical properties. Released novel derivatives and phenolic compounds have a significant role in human and animal health and gut-microbiota interactions, affecting many metabolic pathways. The latest studies have contributed to enzyme-modified extracts and products used for functional, fermented products development and sustainable processes: in particular, nanocellulose, nanocrystals, nanoparticles green synthesis with drug delivery, wound healing, and antimicrobial properties. Even so, enzymes’ incorporation into processes has limitations and is regulated by national and international levels.
Emerging Targeted Therapy for Specific Genomic Abnormalities in Acute Myeloid Leukemia
Authors: Chi Sung-Gi , Minami Yosuke
Published: February 2022

We describe recent updates of existing molecular-targeting agents and emerging novel gene-specific strategies. FLT3 and IDH inhibitors are being tested in combination with conventional chemotherapy for both medically fit patients and patients who are ineligible for intensive therapy. FLT3 inhibitors combined with non-cytotoxic agents, such as BCL-2 inhibitors, have potential therapeutic applicability. The menin-MLL complex pathway is an emerging therapeutic target. The pathway accounts for the leukemogenesis in AML with MLL-rearrangement, NPM1 mutation, and NUP98 fusion genes. Potent menin-MLL inhibitors have demonstrated promising anti-leukemic effects in preclinical studies. The downstream signaling molecule SYK represents an additional target. However, the TP53 mutation continues to remain a challenge. While the p53 stabilizer APR-246 in combination with azacitidine failed to show superiority compared to azacitidine monotherapy in a phase 3 trial, next-generation p53 stabilizers are now under development. Among a number of non-canonical approaches to TP53-mutated AML, the anti-CD47 antibody magrolimab in combination with azacitidine showed promising results in a phase 1b trial. Further, the efficacy was somewhat better in patients with the TP53 mutation. Although clinical evidence has not been accumulated sufficiently, targeting activating KIT mutations and RAS pathway-related molecules can be a future therapeutic strategy.
A Genome-Wide Screen Reveals That Endocytic Genes Are Important for Pma1p Asymmetry during Cell Division in Saccharomyces cerevisiae
Authors: Yoon So-Young , Jang Eunhong , Ko Naho , Kim Minseok , Kim Su Yoon , Moon Yeojin , Nam Jeong-Seok , Lee Sunjae , Jun Youngsoo
Published: February 2022

An asymmetry in cytosolic pH between mother and daughter cells was reported to underlie cellular aging in the budding yeast Saccharomyces cerevisiae; however, the underlying mechanism remains unknown. Preferential accumulation of Pma1p, which pumps cytoplasmic protons out of cells, at the plasma membrane of mother cells, but not of their newly-formed daughter cells, is believed to be responsible for the pH increase in mother cells by reducing the level of cytoplasmic protons. This, in turn, decreases the acidity of vacuoles, which is well correlated with aging of yeast cells. In this study, to identify genes that regulate the preferential accumulation of Pma1p in mother cells, we performed a genome-wide screen using a collection of single gene deletion yeast strains. A subset of genes involved in the endocytic pathway, such as VPS8, VPS9, and VPS21, was important for Pma1p accumulation. Unexpectedly, however, there was little correlation between deletion of each of these genes and the replicative lifespan of yeast, suggesting that Pma1p accumulation in mother cells is not the key determinant that underlies aging of mother cells.
Tryptophan Metabolites Regulate Neuropentraxin 1 Expression in Endothelial Cells
Authors: Vial Romain , Poitevin Stéphane , McKay Nathalie , Burtey Stéphane , Cerini Claire
Published: February 2022

In patients with chronic kidney disease (CKD) and in animal models of CKD, the transcription factor Aryl Hydrocabon Receptor (AhR) is overactivated. In addition to the canonical AhR targets constituting the AhR signature, numerous other genes are regulated by this factor. We identified neuronal pentraxin 1 (NPTX1) as a new AhR target. Belonging to the inflammatory protein family, NPTX1 seems of prime interest regarding the inflammatory state observed in CKD. Endothelial cells were exposed to tryptophan-derived toxins, indoxyl sulfate (IS) and indole-3-acetic acid (IAA). The adenine mouse model of CKD was used to analyze NPTX1 expression in the burden of uremia. NPTX1 expression was quantified by RT-PCR and western blot. AhR involvement was analyzed using silencing RNA. We found that IS and IAA upregulated NPTX1 expression in an AhR-dependent way. Furthermore, this effect was not restricted to uremic indolic toxins since the dioxin 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and the tryptophan photoproduct 6-formylindolo[3,2-b]carbazole (FICZ) do the same. In CKD mice, NPTX1 expression was increased in the aorta. Therefore, NPTX1 is a new target of AhR and further work is necessary to elucidate its exact role during CKD.
Smart Lipid–Polysaccharide Nanoparticles for Targeted Delivery of Doxorubicin to Breast Cancer Cells
Authors: Curcio Manuela , Brindisi Matteo , Cirillo Giuseppe , Frattaruolo Luca , Leggio Antonella , Rago Vittoria , Nicoletta Fiore Pasquale , Cappello Anna Rita , Iemma Francesca
Published: February 2022

In this study, actively-targeted (CD44-receptors) and dual stimuli (pH/redox)-responsive lipid–polymer nanoparticles were proposed as a delivery vehicle of doxorubicin hydrochloride in triple negative breast cancer cell lines. A phosphatidylcholine lipid film was hydrated with a solution of oxidized hyaluronic acid and doxorubicin, chosen as model drug, followed by a crosslinking reaction with cystamine hydrochloride. The obtained spherical nanoparticles (mean diameter of 30 nm) were found to be efficiently internalized in cancer cells by a receptor-mediated endocytosis process, and to modulate the drug release depending on the pH and redox potential of the surrounding medium. In vitro cytotoxicity assays demonstrated the safety and efficacy of the nanoparticles in enhancing the cytotoxic effect of the free anticancer drug, with the IC50 values being reduced by two and three times in MDA-MB-468 and MDA-MB-231, respectively. The combination of self-assembled phospholipid molecules with a polysaccharide counterpart acting as receptor ligand, and stimuli-responsive chemical moieties, was carried out on smart multifunctional nanoparticles able to actively target breast cancer cells and improve the in vitro anticancer activity of doxorubicin.
Neuroprotective Effects of the DPP4 Inhibitor Vildagliptin in In Vivo and In Vitro Models of Parkinson’s Disease
Authors: Pariyar Ramesh , Bastola Tonking , Lee Dae Ho , Seo Jungwon
Published: February 2022

Parkinson’s disease (PD) is characterized by loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) of the midbrain. Restoration of nigrostriatal dopamine neurons has been proposed as a potential therapeutic strategy for PD. Because currently used PD therapeutics only help relieve motor symptoms and do not treat the cause of the disease, highly effective drugs are needed. Vildagliptin, a dipeptidyl peptidase 4 (DPP4) inhibitor, is an anti-diabetic drug with various pharmacological properties including neuroprotective effects. However, the detailed effects of vildagliptin against PD are not fully understood. We investigated the effects of vildagliptin on PD and its underlying molecular mechanisms using a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model and a 1-methyl-4-phenylpyridium (MPP+)-induced cytotoxicity model. Vildagliptin (50 mg/kg) administration significantly attenuated MPTP-induced motor deficits as evidenced by rotarod, pole, and nest building tests. Immunohistochemistry and Western blot analysis revealed that vildagliptin increased tyrosine hydroxylase-positive cells in the SNpc and striatum, which was reduced by MPTP treatment. Furthermore, vildagliptin activated MPTP-decreased PI3k/Akt and mitigated MPTP-increased ERK and JNK signaling pathways in the striatum. Consistent with signaling transduction in the mouse striatum, vildagliptin reversed MPP+-induced dephosphorylation of PI3K/Akt and phosphorylation of ERK and JNK in SH-SY5Y cells. Moreover, vildagliptin attenuated MPP+-induced conversion of LC3B-II in SH-SY5Y cells, suggesting its role in autophagy inhibition. Taken together, these findings indicate that vildagliptin has protective effects against MPTP-induced motor dysfunction by inhibiting dopaminergic neuronal apoptosis, which is associated with regulation of PI3k/Akt, ERK, and JNK signaling transduction. Our findings suggest vildagliptin as a promising repurposing drug to treat PD.
Discovery of SARS-CoV-2 3CLPro Peptidomimetic Inhibitors through the Catalytic Dyad Histidine-Specific Protein–Ligand Interactions
Authors: Wang Yaxin , Xu Binghong , Ma Sen , Wang Hao , Shang Luqing , Zhu Cheng , Ye Sheng
Published: February 2022

As the etiological agent for the coronavirus disease 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenges the ongoing efforts of vaccine development and drug design. Due to the accumulating cases of breakthrough infections, there are urgent needs for broad-spectrum antiviral medicines. Here, we designed and examined five new tetrapeptidomimetic anti-SARS-CoV-2 inhibitors targeting the 3C-Like protease (3CLPro), which is highly conserved among coronaviruses and essential for viral replications. We significantly improved the efficacy of a ketoamide lead compound based on high-resolution co-crystal structures, all-atom simulations, and binding energy calculations. The inhibitors successfully engaged the catalytic dyad histidine residue (H41) of 3CLPro as designed, and they exhibited nanomolar inhibitory capacity as well as mitigated the viral loads of SARS-CoV-2 in cellular assays. As a widely applicable design principle, our results revealed that the potencies of 3CLPro-specific drug candidates were determined by the interplay between 3CLPro H41 residue and the peptidomimetic inhibitors.
Automatic Hemiplegia Type Detection (Right or Left) Using the Levenberg-Marquardt Backpropagation Method
Authors: Christou Vasileios , Arjmand Alexandros , Dimopoulos Dimitrios , Varvarousis Dimitrios , Tsoulos Ioannis , Tzallas Alexandros T. , Gogos Christos , Tsipouras Markos G. , Glavas Evripidis , Ploumis Avraam , Giannakeas Nikolaos
Published: February 2022

Hemiplegia affects a significant portion of the human population. It is a condition that causes motor impairment and severely reduces the patient’s quality of life. This paper presents an automatic system for identifying the hemiplegia type (right or left part of the body is affected). The proposed system utilizes the data taken from patients and healthy subjects using the accelerometer sensor from the RehaGait mobile gait analysis system. The collected data undergo a pre-processing procedure followed by a feature extraction stage. The extracted features are then sent to a neural network trained by the Levenberg-Marquardt backpropagation (LM-BP) algorithm. The experimental part of this research involved creating a custom-created dataset containing entries taken from ten healthy and twenty non-healthy subjects. The data were taken from seven different sensors placed in specific areas of the subjects’ bodies. These sensors can capture a three-dimensional (3D) signal using the accelerometer, magnetometer, and gyroscope device types. The proposed system used the signals taken from the accelerometers, which were split into 2-sec windows. The proposed system achieved a classification accuracy of 95.12% and was compared with fourteen commonly used machine learning approaches.
Multivariate Linear Regression Models to Predict Monomer Poisoning Effect in Ethylene/Polar Monomer Copolymerization Catalyzed by Late Transition Metals
Authors: Zhao Wei , Liu Zhihao , Zhao Yanan , Luo Yi , He Shengbao
Published: February 2022

This study combined density functional theory (DFT) calculations and multivariate linear regression (MLR) to analyze the monomer poisoning effect in ethylene/polar monomer copolymerization catalyzed by the Brookhart-type catalysts. The calculation results showed that the poisoning effect of polar monomers with relatively electron-deficient functional groups is weaker, such as ethers, and halogens. On the contrary, polar monomers with electron-rich functional groups (carbonyl, carboxyl, and acyl groups) exert a stronger poisoning effect. In addition, three descriptors that significantly affect the poisoning effect have been proposed on the basis of the multiple linear regression model, viz., the chemical shift of the vinyl carbon atom and heteroatom of polar monomer as well as the metal-X distance in the σ-coordination structure. It is expected that these models could guide the development of efficient catalytic copolymerization system in this field.
Leukocyte-Mediated Cardiac Repair after Myocardial Infarction in Non-Regenerative vs. Regenerative Systems
Authors: Peterson Elizabeth Anne , Sun Jisheng , Wang Jinhu
Published: February 2022

Innate and adaptive leukocytes rapidly mobilize to ischemic tissues after myocardial infarction in response to damage signals released from necrotic cells. Leukocytes play important roles in cardiac repair and regeneration such as inflammation initiation and resolution; the removal of dead cells and debris; the deposition of the extracellular matrix and granulation tissue; supporting angiogenesis and cardiomyocyte proliferation; and fibrotic scar generation and resolution. By organizing and comparing the present knowledge of leukocyte recruitment and function after cardiac injury in non-regenerative to regenerative systems, we propose that the leukocyte response to cardiac injury differs in non-regenerative adult mammals such as humans and mice in comparison to cardiac regenerative models such as neonatal mice and adult zebrafish. Specifically, extensive neutrophil, macrophage, and T-cell persistence contributes to a lengthy inflammatory period in non-regenerative systems for adverse cardiac remodeling and heart failure development, whereas their quick removal supports inflammation resolution in regenerative systems for new contractile tissue formation and coronary revascularization. Surprisingly, other leukocytes have not been examined in regenerative model systems. With this review, we aim to encourage the development of improved immune cell markers and tools in cardiac regenerative models for the identification of new immune targets in non-regenerative systems to develop new therapies.