bluuebunny/crossref_metadata_2025 huggingface dataset:
CD44 Is Associated with Poor Prognosis of ccRCC and Facilitates ccRCC Cell Migration and Invasion through HAS1/MMP9
Authors: Du Tan , Wu Zonglong , Wu Yaqian , Liu Yunchong , Song Yimeng , Ma Lulin
Published: July 2023

Background: In many solid tumors, CD44 has been identified as a cancer stem cell marker as well as an important molecular in cancer progression and metastasis, making it attractive for potential therapeutic applications. However, our knowledge of the biological function and mechanism of CD44 in clear cell renal cell carcinoma (ccRCC) is limited. Methods: In this study, the expression, prognostic values and functional enrichment analysis of CD44 in ccRCC were analyzed using public databases. Quantitative real-time PCR (qRT-PCR), Western blotting, and immunohistochemical (IHC) assays were taken to detect CD44 expression in ccRCC tissues. The effects of CD44 on the proliferation, migration and invasion of ccRCC cells were investigated by gain-of-function and loss-of-function experiments. Subcutaneous models further confirmed the role of CD44 in tumor growth. The relationship between CD44, HAS1 and MMP9 was investigated to uncover the regulatory mechanism of CD44 in ccRCC. Results: CD44 was significantly upregulated in ccRCC and associated with poor overall survival (OS). Based on the functional enrichment analysis and PPI network, we found that CD44 had associations with ECM interaction and focal adhesion pathway. Clinical ccRCC sample validation revealed that CD44 mRNA and protein expression were significantly increased in ccRCC tissues, and strong CD44 staining was observed in four metastatic ccRCC cases. In vitro experiments showed that CD44 overexpression promoted cell proliferation, migration and invasion. In vivo experiments also demonstrated that CD44 overexpression accelerated tumor formation in mice. Finally, we found that CD44 regulates the expression of HAS1 in ccRCC, which is essential for the secretion of MMP9 and cell migratory ability. Conclusion: The upregulation of CD44 mRNA and protein expressions in ccRCC is indicative of unfavorable clinical prognoses. The CD44/HAS1/MMP9 axis is believed to exert a significant influence on the regulation of ECM degradation and ccRCC metastasis.
Evaluation of Folate-Functionalized Nanoparticle Drug Delivery Systems—Effectiveness and Concerns
Authors: Ibrahim Muhammad Aiman Irfan , Othman Rozana , Chee Chin Fei , Ahmad Fisol Faisalina
Published: July 2023

Targeting folate receptors is a potential solution to low tumor selectivity concerning conventional chemotherapeutics. Apart from antibody–drug conjugates, folate-functionalized nanoparticle drug delivery systems are interesting to be explored due to many advantages, yet currently, none seems to enter the clinical trials. Multiple in vitro evidence is available to support its efficacy compared to the non-targeting carrier and free drug formulation. Additionally, several studies pointed out factors affecting its effectiveness, including surface properties and endosomal trapping. However, in vivo biodistribution studies revealed issues that may arise from folate receptor targeting, including rapid liver uptake, subsequently reducing the nanoparticles’ tumor uptake. This issue may be due to the folate receptor β expressed by the activated macrophages in the liver; route of administration and tumor location might also influence the targeting effectiveness. Moreover, it is perplexing to generalize nanoparticles reported from various publications, primarily due to the different formulations, lack of characterization, and experimental settings, making it harder to determine the accurate factor influencing targeting effectiveness.
Bipolar Androgen Therapy: When Excess Fuel Extinguishes the Fire
Authors: Nabavi Nima , Mahdavi Seied Rabi , Ardalan Mohammad Afshar , Chamanara Mohsen , Mosaed Reza , Lara Aline , Bastos Diogo , Harsini Sara , Askari Emran , Velho Pedro Isaacsson , Bagheri Hamed
Published: July 2023

Androgen deprivation therapy (ADT) remains the cornerstone of advanced prostate cancer treatment. However, the progression towards castration-resistant prostate cancer is inevitable, as the cancer cells reactivate androgen receptor signaling and adapt to the castrate state through autoregulation of the androgen receptor. Additionally, the upfront use of novel hormonal agents such as enzalutamide and abiraterone acetate may result in long-term toxicities and may trigger the selection of AR-independent cells through “Darwinian” treatment-induced pressure. Therefore, it is crucial to develop new strategies to overcome these challenges. Bipolar androgen therapy (BAT) is one such approach that has been devised based on studies demonstrating the paradoxical inhibitory effects of supraphysiologic testosterone on prostate cancer growth, achieved through a variety of mechanisms acting in concert. BAT involves rapidly alternating testosterone levels between supraphysiological and near-castrate levels over a period of a month, achieved through monthly intramuscular injections of testosterone plus concurrent ADT. BAT is effective and well-tolerated, improving quality of life and potentially re-sensitizing patients to previous hormonal therapies after progression. By exploring the mechanisms and clinical evidence for BAT, this review seeks to shed light on its potential as a promising new approach to prostate cancer treatment.
DRD2, DRD3, and HTR2A Single-Nucleotide Polymorphisms Involvement in High Treatment Resistance to Atypical Antipsychotic Drugs
Authors: Del Casale Antonio , Simmaco Maurizio , Modesti Martina Nicole , Zocchi Clarissa , Arena Jan Francesco , Bilotta Irene , Alcibiade Alessandro , Sarli Giuseppe , Cutillo Lorenzo , Antonelli Giulia , La Spina Enrico , De Luca Ottavia , Preissner Robert , Borro Marina , Gentile Giovanna , Girardi Paolo , Pompili Maurizio
Published: July 2023

Background: The objective of this study was to investigate the DRD2 rs1800497, rs1799732, rs1801028, DRD3 rs6280, and HTR2A rs6314, rs7997012, and rs6311 single-nucleotide polymorphism (SNP) correlations with resistance to second-generation antipsychotics (SGAs) in a real-world sample of patients with treatment-resistant mental disorders. Methods: We divided 129 participants into a high treatment resistance (HTR) group (current treatment with two SGAs, or clozapine, or classic neuroleptics for a failure of previous SGAs trials) and a low treatment resistance (LTR) group (current treatment with one atypical antipsychotic). We used Next-Generation Sequencing on DNA isolated from peripheral blood samples to analyze the polymorphisms. We performed logistic regression to search for predictors of HTR membership. Results: A diagnosis of schizophrenia significantly predicted the HTR membership compared to other diagnoses. Other predictors were the DRD3 rs6280 C|T (OR = 22.195) and T|T (OR = 18.47) vs. C|C, HTR2A rs7997012 A|G vs. A|A (OR = 6.859) and vs. G|G (OR = 2.879), and DRD2 rs1799732 I|I vs. D|I (OR = 12.079) genotypes. Conclusions: A diagnosis of schizophrenia and the DRD2 rs1799732, DRD3 rs6280, and HTR2A rs7997012 genotypes can predict high treatment resistance to SGAs.
Correction: Musa et al. An Electrochemical Screen-Printed Sensor Based on Gold-Nanoparticle-Decorated Reduced Graphene Oxide–Carbon Nanotubes Composites for the Determination of 17-β Estradiol. Biosensors 2023, 13, 491
Authors: Musa Auwal M. , Kiely Janice , Luxton Richard , Honeychurch Kevin C.
Published: July 2023

In the original publication [...]
Responsibility as an Ethics and Sustainability Element during the Pandemic
Authors: Țigan Eugenia , Lungu Monica , Brînzan Oana , Blaga Radu Lucian , Milin Ioana Anda , Gavrilaș Simona
Published: July 2023

This article addresses two perspectives of responsibility: as an element of taking action and as ethics towards one’s fellow citizens and the environment. These aspects have been used since the beginning of the pandemic. In this context, we wanted to determine the mechanism that triggers increased responsibility. We have considered two possible initial processes: one external and one learned from the family in early childhood or trained/learned during studies. Based on that, three concerns were brought to our attention. First, we targeted the impact of the pandemic on respondents’ degree of responsibility for their livelihoods. Second, we wanted to determine to what extent the new situation increased the interviewee’s involvement only in some activities. The third statement called into question the causal relation between the influence of extrinsic elements on a highly educated person’s degree of responsibility. The focus group was the active population of the industrial sector in the North–West border area of Romania. The data obtained are the result of the sociological survey implementation. The people in the region were subject to several new limiting external factors. The results show that the unique challenges up to that point made them more accountable for their actions in a situation that affected them directly (pandemic). It also can be underlined that lifelong education is important in forming healthy principles of responsibility.
Public Database of Cracks Images in Mortar Coating with Different Types of Surface Finishes
Authors: Garcia Sobrinho Renner de Assis , Piauhy Neto Franklin , Fernandes Henrique
Published: July 2023

The use of technology, such as artificial intelligence (AI), in production processes has been optimizing several industrial realities. In civil construction, AI can be used in different applications, one of which is building inspection. One of the difficulties in developing this type of study is the low number of public image databases that represent more general aspects of building wear. In view of this, the main objective of this research was to set up a public database of images of cracks in mortar coating, considering different types of surface finish—smooth type, scrapped type, and rough type. A database was created with 33,088 images that went through a systematic labeling process based on classes defined in the study. Network training was carried out through transfer learning using the VGG16 in different groupings of finishes. It was found that the training accuracy varies according to surface finish and data balancing. The finish of the scrapped type was the one that presented the lowest accuracy. The database presented several types of noise and was unbalanced in all categories defined in the labeling. In this way, it was possible to create a database that represented possible situations to be found in real inspections.
Establishment of Novel Mouse Model of Dietary NASH Rapidly Progressing into Liver Cirrhosis and Tumors
Authors: Zheng Qianqian , Kawaguchi Masaya , Mikami Hayato , Diao Pan , Zhang Xuguang , Zhang Zhe , Nakajima Takero , Iwadare Takanobu , Kimura Takefumi , Nakayama Jun , Tanaka Naoki
Published: July 2023

Non-alcoholic steatohepatitis (NASH), which is the most severe manifestation of non-alcoholic fatty liver disease (NAFLD), has been recognized as a major hepatocellular carcinoma (HCC) catalyst. However, the molecular mechanism of NASH-liver fibrosis-HCC sequence remains unclear and a specific and effective treatment for NASH has not yet been established. The progress in this field depends on the availability of reliable preclinical models which show the steady progression to NASH, liver cirrhosis, and HCC. However, most of the NASH mouse models that have been described to date develop NASH generally for more than 24 weeks and there is an uncertainty of HCC development. To overcome such shortcomings of experimental NASH studies, we established a novel NASH-HCC mouse model with very high reproducibility, generality, and convenience. We treated male C57BL/6J mice with a newly developed choline-deficient and methionine-restricted high-fat diet, named OYC-NASH2 diet, for 60 weeks. Treatment of OYC-NASH2 diet for 3 weeks revealed marked steatosis, lobular inflammation, and fibrosis, histologically diagnosed as NASH. Liver cirrhosis was observed in all mice with 48-week treatment. Liver nodules emerged at 12 weeks of the treatment, > 2 mm diameter liver tumors developed in all mice at 24 weeks of the treatment and HCC appeared after 36-week treatment. In conclusion, our rapidly progressive and highly reproducible NASH-liver cirrhosis-HCC model is helpful for preclinical development and research on the pathogenesis of human NAFLD-NASH-HCC. Our mouse model would be useful for the development of novel chemicals for NASH-HCC-targeted therapies.
Efficacy and Safety of Rechallenge with BRAF/MEK Inhibitors in Advanced Melanoma Patients: A Systematic Review and Meta-Analysis
Authors: Priantti Jonathan N. , Vilbert Maysa , Madeira Thiago , Moraes Francisco Cezar A. , Hein Erica C. Koch , Saeed Anwaar , Cavalcante Ludimila
Published: July 2023

This systematic review and meta-analysis aims to evaluate the efficacy and safety of rechallenging advanced melanoma patients with BRAFi/MEKi. Seven studies, accounting for 400 patients, were included. Most patients received immunotherapy before the rechallenge, and 79% underwent rechallenge with the combination of BRAFi/MEKi. We found a median progression-free survival of 5 months and overall survival of 9.8 months. The one-year survival rate was 42.63%. Regarding response, ORR was 34% and DCR 65%. There were no new or unexpected safety concerns. Rechallenge with BRAFi/MEKi can improve outcomes in advanced melanoma patients with refractory disease. These findings have significant implications for clinical practice, particularly in the setting of progressive disease in later lines and limited treatment options.
Computed Tomography Utilization in the Management of Children with Mild Head Trauma
Authors: Leva Ernest , Do Minh-Tu , Grieco Rachael , Petrova Anna
Published: July 2023

This study demonstrates the trend of computed tomography (CT) usage for children with mild traumatic brain injury (mTBI) in the context of the initiation of the Safe CT Imaging Collaborative Initiative to promote the Pediatric Emergency Care Applied Research Network (PECARN) rules at the acute care hospitals in New Jersey. We used administrative databases of 10 children’s and 59 general hospitals to compare CT rates before 2014–2015, during 2016, and after the initiation of the program (2017–2019). The CT usage rates at baseline and the end of surveillance in children’s hospitals (19.2% and 14.2%) were lower than in general hospitals (36.7% and 21.0%), p < 0.0001. The absolute mean difference from baseline to the end of surveillance in children’s hospitals was 5.1% compared to a high of 9.7% in general hospitals, medium-high with 13.2%, and 14.0% in a medium volume of pediatric patients (p < 0.001–0.0001). The time-series model demonstrates a positive trend of CT reduction in pediatric patients with mTBI within four years of the program’s implementation (p < 0.03–0.001). The primary CT reduction was recorded during the year of program implementation. Regression analysis revealed the significant role of a baseline CT usage rate in predicting the level of CT reduction independent of the volume of pediatric patients and type of hospital.
The Wetting Behavior of Water Droplets on Silane and Silane/GO-Modified Ettringite Surfaces: Insights into Molecular Dynamics Simulations
Authors: Li Mengmeng , Zheng Heping , Duan Yuying , Hou Dongshuai , Wang Pan , Pang Bo , Li Shaochun , Jin Zuquan
Published: July 2023

The use of rapid-hardening cementitious materials for the emergency repair of critical infrastructure in coastal environments is becoming increasingly widespread, and concrete surface hydrophobic protection treatment is equally necessary to improve the durability of both new and old concrete. Among them, silane-based hydrophobic materials play an important role in concrete hydrophobic protection. Graphene oxide (GO)-modified silane materials can significantly improve the hydrophobic performance of coatings, but the hydrophobic mechanism of coatings modifying the sulfoaluminate cement’s main hydration product ettringite (AFt) has not yet been explored. In this study, molecular dynamics simulations were conducted to investigate the wetting properties of water droplets on the surface of AFt, isobutyltriethoxy silane (IBTS)-modified AFt, and IBTS/GO-modified AFt. It was found that the AFt substrate had good hydrophilicity, and the droplets could wet the interface through Ca-O ionic bonds and H-bonds. The IBTS coating initially impeded droplet wetting, but the adsorption stability of IBTS on the AFt substrate surface was poor under droplet action, leading to droplet penetration and dispersion of the IBTS coating on the AFt surface. However, the IBTS/GO coating significantly restricted droplet wetting due to the stable adsorption of GO on the AFt surface and the strong stability of hydrogen bonds between IBTS and GO. In conclusion, selecting a suitable bridging material between AFt and silane is crucial for improving the hydrophobic stability of silane coatings on sulfoaluminate cement materials.
Influence of Surface Roughness on Nanocrystalline Diamond Films Deposited by Distributed Antenna Array Microwave System on TA6V Substrates
Authors: Valinattaj Omran Azadeh , Mahi Chaimaa , Vayron Romain , Falentin-Daudré Céline , Bénédic Fabien
Published: July 2023

In this study, the characteristics of nanocrystalline diamond films synthesized at low surface temperature on Ti-6Al-4V (TA6V) substrates using a distributed antenna array microwave reactor aiming at biomedical applications were investigated. The surface roughness of the TA6V substrates is varied by scratching with emery paper of 1200, 2400, 4000 polishing grit. Nanocrystalline diamond (NCD) coatings with morphology, purity, and microstructure comparable to those obtained on silicon substrates usually employed in the same reactor and growth conditions are successfully achieved whatever the polishing protocol. However, the latter has a significant effect on the roughness parameters and hardness of the NCD films. The use of the finest polishing grit thus permits us to enhance the hardness value, which can be related to the work-hardening phenomenon arising from the polishing process.
Diagnostic Role of Native T1 Mapping Compared to Conventional Magnetic Resonance Techniques in Cardiac Disease in a Real-Life Cohort
Authors: Aquaro Giovanni Donato , Monastero Silvia , Todiere Giancarlo , Barison Andrea , De Gori Carmelo , Grigoratos Crysanthos , Parisella Maria Luisa , Faggioni Lorenzo , Cioni Dania , Lencioni Riccardo , Neri Emanuele
Published: July 2023

We sought to compare native T1 mapping to conventional late gadolinium enhancement (LGE) and T2-STIR techniques in a cohort of consecutive patients undergoing cardiac MRI (CMR). CMR was performed in 323 patients, 206 males (64%), mean age 54 ± 8 years, and in 27 age- and sex- matched healthy controls. In T2-STIR images, myocardial hyperintensity suggesting edema was found in 41 patients (27%). LGE images were positive in 206 patients (64%). T1 mapping was abnormal in 171 (49%). In 206 patients (64%), a matching between LGE and native T1 was found. T1 was abnormal in 32 out of 41 (78%) with edema in T2-STIR images. Overall, LGE and/or T2-STIR were abnormal in 209 patients, whereas native T1 was abnormal in 154 (52%). Conventional techniques and T1 mapping were concordant in 208 patients (64%). In 39 patients, T1 mapping was positive despite negative conventional techniques (12%). T1 mapping was able in conditions with diffuse myocardial damage such as cardiac amyloidosis, scleroderma, and Fabry disease (additive role in 42%). In contrast, T1 mapping was less effective in cardiac disease with regional distribution of myocardial damage such as myocardial infarction, HCM, and myocarditis. In conclusion, conventional LGE/T2-STIR and T1 mapping are complementary techniques and should be used together in every CMR examination.
Kaniadakis’s Information Geometry of Compositional Data
Authors: Pistone Giovanni , Shoaib Muhammad
Published: July 2023

We propose to use a particular case of Kaniadakis’ logarithm for the exploratory analysis of compositional data following the Aitchison approach. The affine information geometry derived from Kaniadakis’ logarithm provides a consistent setup for the geometric analysis of compositional data. Moreover, the affine setup suggests a rationale for choosing a specific divergence, which we name the Kaniadakis divergence.
Using Stop Motion Animations to Activate and Analyze High School Students’ Intuitive Resources about Reaction Mechanisms
Authors: Pölloth Benjamin , Schäffer Dominik , Schwarzer Stefan
Published: July 2023

Mechanisms are part of the high school curriculum in many countries. Although research shows that university students struggle with mechanistic reasoning, very little is known about learning mechanisms in high school. Understanding the cognitive resources that high school students intuitively activate is critical to fostering meaningful learning. A readily available tool for modeling dynamic processes are stop-motion animations (SMAs). In this study, 55 high school students were asked to create SMAs of their intuitive ideas of nucleophilic substitution reactions. In a second step, the students evaluated two pre-made SMAs modeling the textbook-based stepwise (SN1) and concerted (SN2) mechanisms. Within the student-generated SMAs, SN1- and SN2-like mechanisms were equally distributed; after viewing the textbook-style animations, most students rated an SN2 mechanism as more likely. However, no group modeled both types of mechanisms or reasoned that both mechanisms were possible. Students used diverse chemical concepts as well as plausibility in their reasoning. However, simultaneous movement of atoms, conformational changes, and the idea of competing and boundary mechanisms do not seem intuitive. In conclusion, SMAs were found to be an appropriate tool for activating, analyzing, and discussing students’ resources “on the fly”, and these ideas can serve as a starting point for promoting productive mechanistic reasoning.
A Low Jitter, Wideband Clock Generator for Multi-Protocol Data Communications Applications
Authors: Jiang Yingdan , Yu Yang , Tang Lu , Yang Junhao , Lu Yujia , Yu Zongguang
Published: July 2023

This paper presents a charge-pump phase-locked loop (PLL) frequency-synthesizer-based low-jitter wideband clock generator for multi-protocol data communications applications. Automatic frequency calibration (AFC) using linear variable time window technology and modified multi-modulus dividers (MMD) based on sub-multi-modulus dividers (SMMD) are developed for faster locking, lower jitter, and implementation of multi-protocol data communications applications. The clock generator is fabricated in 0.18 μm CMOS technology. The measured division ratio of the multi-modulus divider ranges from 1.875 to 25, and the output frequency is 46.875~625 MHz. The lock time does not exceed 30 μs, while jitter is less than 500 fs.
Remote Bridge Inspection and Actual Bridge Verification Based on 4G/5G Communication Environments
Authors: Yoshikura Mai , Minami Takahiro , Fukuoka Tomotaka , Fujiu Makoto , Takayama Jyunichi
Published: July 2023

The close-up visual inspection of bridges faces several problems, including a lack of financial resources and human personnel. Hence, there has been increasing use of artificial intelligence (AI) and information and communications technology (ICT) to solve them. We previously investigated remote inspection—in which skilled engineers provided on-site support from a remote location—with the aim of reducing the labor required for on-site work and addressing the lack of personnel through the use of AI and ICT. Sharing images of bridges from inspection sites to remote locations via the Internet enables remote assessment of the sites and the ability to consider and diagnose damage. Mobile communications can be used to upload images, although the volume of image data required for inspection can be enormous and take considerable time to upload. Consequently, in this study, we investigated image uploads using 5G communication—that is, the fifth-generation technology standard for broadband cellular networks. Moreover, we measured the upload times when using 4G and 5G services and examined their operation based on differences in the communication environments. We concluded that the simulated remote inspection can be efficiently performed by adjusting the inspection method to the communication environment.
Digital Twin for Math Education: A Study on the Utilization of Games and Gamification for University Mathematics Education
Authors: Lee Jung Yeop , Pyon Chong Un , Woo Jiyoung
Published: July 2023

Gamification has emerged as a promising strategy for engaging students and enhancing learning outcomes across various disciplines, including education. It involves incorporating game-like elements into non-game contexts to motivate and engage users. Extensive research has demonstrated the positive impact of gamification on student engagement, motivation, and learning outcomes in diverse subjects, including mathematics. Nevertheless, there is a paucity of research on the effectiveness of gamification in mathematics education. To bridge this gap, we investigated the effects of gamification in college mathematics education, with a specific emphasis on students from liberal arts backgrounds who may lack foundational mathematical knowledge. In this work, we designed and implemented a gamified mathematics course tailored for liberal arts students in a Korean university. Leveraging digital twin technology for game and gamification, we created an immersive learning environment that allows students to visualize mathematical concepts through games, pair gamification factors on physical and virtual worlds, and engage in interactive problem-solving activities. The effectiveness of this gamified course in improving students’ engagement, motivation, and learning outcomes was thoroughly evaluated. Digital twin technology, in conjunction with gamification, holds immense potential to revolutionize the way mathematics is taught and learned, making it more accessible, interactive, and engaging for students from diverse educational backgrounds.
A Comparatively Experimental Study on the Performance and Emission Characteristics of a Diesel Engine Fueled with Tung Oil-Based Biodiesel Blends (B10, B20, B50)
Authors: Mu Zhiyue , Fu Jianqin , Zhou Feng , Yuan Kainan , Yu Juan , Huang Dan , Cui Zhuangping , Duan Xiongbo , Liu Jingping
Published: July 2023

In this paper, the performance and emission characteristics of a diesel engine were investigated with varying ratios of tung oil-based biodiesel blends (B10, B20, and B50) and neat diesel under different operating conditions. The experimental results showed that the addition of biodiesel blends had different effects on engine power and torque depending on the blend ratio. B10 displayed a slight increase in power and torque, which increased by 1.9% and 6.6%. At the same time, B20 and B50 showed declines slightly. The fuel consumption rate increased slightly with an increasing percentage of biodiesel added. In general, all the blends exhibited significantly lower emissions of CO, NOX, HC, and smoke compared to neat diesel. B10 displayed the most notable reduction of CO emissions, with a 42.86% decrease at medium to high loads. NOX emissions of tung oil-based biodiesel blends were reduced at all load conditions except for B50. In addition, HC emissions were all reduced, especially for B20, which led to a 27.54% reduction at 50% load. Among all the tested blends, B50 showed the greatest decrease in smoke emissions of 38.05% compared to neat diesel at 2000 rpm. The research concluded that using biodiesel fuels from renewable resources, such as tung oil, presents a promising environmentally friendly alternative fuel option.
Prediction of Gas Hydrate Formation in the Wellbore
Authors: Duan Xinyue , Zuo Jiaqiang , Li Jiadong , Tian Yu , Zhu Chuanyong , Gong Liang
Published: July 2023

The formation of gas hydrates due to temperature and pressure changes during gas storage in the wellbore poses significant danger, necessitating the prediction of temperature and pressure distribution as well as of hydrate formation locations. We establish a temperature model that couples total thermal resistance and temperature in the wellbore-stratum composite medium system. Utilizing the two-phase pressure model alongside the temperature model, we conduct coupling calculations of temperature and pressure. Based on both temperature and pressure distribution within the wellbore and hydrate formation curve, we predict hydrate formation regions during production and analyze factors influencing temperature and pressure distribution. Results indicate that gas production rate and specific gravity of natural gas are major influencers on wellbore temperature and pressure distribution, while production time has minimal impact.
The Architecture for Testing Central Heating Control Algorithms with Feedback from Wireless Temperature Sensors
Authors: Markiewicz Michał , Skała Aleksander , Grela Jakub , Janusz Szymon , Stasiak Tadeusz , Latoń Dominik , Bielecki Andrzej , Bańczyk Katarzyna
Published: July 2023

The energy consumption of buildings is a significant contributor to overall energy consumption in developed countries. Therefore, there is great demand for intelligent buildings in which energy consumption is optimized. Online control is a crucial aspect of such optimization. The implementation of modern algorithms that take advantage of developments in information technology, artificial intelligence, machine learning, sensors, and the Internet of Things (IoT) is used in this context. In this paper, an architecture for testing central heating control algorithms as well as the control algorithms of the heating system of the building is presented. In particular, evaluation metrics, the method for seamless integration, and the mechanism for real-time performance monitoring and control are put forward. The proposed tools have been successfully tested in a residential building, and the conducted tests confirmed the efficiency of the proposed solution.
Production of 2,3-Butanediol by S. cerevisiae L7 in Fed-Batch Fermentation with Optimized Culture Conditions
Authors: Ao Guoxu , Sun Shanshan , Liu Lei , Guo Yuhao , Tu Xiujun , Ge Jingping , Ping Wenxiang
Published: July 2023

As a chemical platform, 2,3-Butanediol (2,3-BD) has been widely applied in various industrial fields. In this study, to enhance the production of 2,3-BD by Saccharomyces cerevisiae L7, Plackett–Burman (PB) multifactorial design, the steepest climb test and central composite design (CCD) were employed to optimize the culturing conditions of S. cerevisiae L7. The results show that acetic acid, peptone and glucose were contributing factors for 2,3-BD production. Subsequently, a satisfactory production of 2,3-BD (13.52 ± 0.12 g/L) was reached under optimal conditions, which was 3.12 times higher than before optimization. Furthermore, fed-batch fermentation was carried out under optimized culture conditions, and a higher production and yield efficiency of 2,3-BD were achieved (21.83 ± 0.56 g/L and 0.15 ± 0.01 g/g, respectively) when glucose (20 g/L) and acetic acid (0.2 g/L) were added at 12, 24, 36, 48 and 60 h. Therefore, the production and yield efficiency of 2,3-BD were higher than those without fed-batch fermentation (61.46% and 58.51%, respectively). These results provide good support and a technical foundation for the large-scale industrial production of 2,3-BD by Saccharomyces cerevisiae.
Effects of Main Nutrient Sources on Improving Monascus Pigments and Saccharifying Power of Monascus purpureus in Submerged Fermentation
Authors: Huang Yingying , Chen Jiashi , Chen Qing , Yang Chenglong
Published: July 2023

Hong Qu (HQ), obtained through fermentation of various grains using Monascus spp., has been widely utilized as the main and characteristic initial saccharification and traditional fermentation starter in the food brewing industry. The quality, color, and flavor of HQ and HQ wine are closely related to the saccharifying power (SP) and Monascus pigments (MPs) of Monascus spp. In this study, to optimize the culture medium in submerged fermentation by M. purpureus G11 for improving SP and MPs, the effects of carbon source, nitrogen source, inorganic salts, and vitamins on SP activity and biosynthesis of MPs were explored through single-factor analysis and response surface Box–Behnken experiments. The results showed that the optimal medium composition was 6.008% rice powder, 1.021% peptone, 0.0049% CuSO4, and 0.052% vitamin B1. Validation experiments performed under the optimized fermentation conditions showed a significant increase in MPs and SP by 14.91% and 36.24%, with maximum MPs and SP reaching 112.61 and 365.12 u/mL, respectively. This study provides a theoretical basis for enhancing MPs and SP in M. purpureus for HQ production, to improve the production efficiency and shorten the production cycle of HQ-related fermentation products.
Fiber Spinning of Polyacrylonitrile Terpolymers Containing Acrylic Acid and Alkyl Acrylates
Authors: Skvortsov Ivan Yu. , Kuzin Mikhail S. , Vashchenko Andrey F. , Toms Roman V. , Varfolomeeva Lydia A. , Chernikova Elena V. , Shambilova Gulbarshin K. , Kulichikhin Valery G.
Published: July 2023

Terpolymers of acrylonitrile with acrylic acid and alkyl acrylates, including methyl-, butyl-, 2-ethylhexyl-, and lauryl acrylates, were synthesized using the reversible addition–fragmentation chain transfer method. In this study, the focus was on the investigation of the impact of different monomer addition methods (continuous and batch) on both the rheological behavior of the spinning solutions and the mechanical properties of the resulting fibers. Our findings revealed that the method of monomer addition, leading either to non-uniform copolymers or to a uniform distribution, significantly influences the rheological properties of the concentrated solutions, surpassing the influence of the alkyl-acrylate nature alone. To determine the optimal spinning regime, we examined the morphology and mechanical properties at different stages of fiber spinning, considering spin-bond and orientation drawings. The fiber properties were found to be influenced by both the nature and introducing method of the alkyl-acrylate comonomer. Remarkably, the copolymer with methyl acrylate demonstrates the maximum drawing ratios and fiber tensile strength, reaching 1 GPa. Moreover, we discovered that continuous monomer addition allows for reaching the higher drawing ratios and superior fiber strength compared to the batch method.
Decomposition Rates of Suppression-Produced Fish Carcasses in a Large, Deep, High-Elevation Lake in North America
Authors: Glassic Hayley C. , Guy Christopher S. , Tronstad Lusha M. , Briggs Michelle A. , Albertson Lindsey K. , Lujan Dominique R. , Koel Todd M.
Published: July 2023

The decomposition of vertebrates in lake ecosystems has been largely understudied despite being a vital part of ecosystem processes. Invasive lake trout (Salvelinus namaycush) invaded Yellowstone Lake and caused a decline in the native Yellowstone cutthroat trout (Oncorhynchus clarkii bouvieri) population. To restore Yellowstone cutthroat trout, lake trout were suppressed by gillnetting annually since 1995 and has continued to present, with most carcasses deposited in the profundal zone (>70 m). As a part of suppression management, a fraction of carcasses from gillnetting were ground and placed on littoral spawning sites (causing lake trout embryo mortality via hypoxia). We conducted experiments (2018 and 2019) to determine how carcass state (i.e., whole vs. ground) and location of deposition (i.e., profundal or littoral) affected decomposition rates. Whole carcasses in the depths of Yellowstone Lake decomposed nine times slower (rate of decay, k = −0.0075 day−1; 95% CI = −0.0063–−0.0089) than ground carcasses in the littoral zone (k = −0.0679 day−1; 95% CI = −0.0590–−0.0768). Whole carcasses had a half-life of 91 days while ground carcasses had a half-life of 10 days. We showed that carcass state and location cause a differential decomposition for lake trout carcasses in Yellowstone Lake. Understanding carcass persistence in lakes can inform the management of suppression-produced carcasses in large lakes and provide insight into potential effects of carcass deposition from other sources, such as spawning events or fish kills, on nutrient cycling.
Phytochemical Constituents, Antimicrobial Properties and Bioactivity of Marine Red Seaweed (Kappaphycus alvarezii) and Seagrass (Cymodocea serrulata)
Authors: Das Deep , Arulkumar Abimannan , Paramasivam Sadayan , Lopez-Santamarina Aroa , del Carmen Mondragon Alicia , Miranda Lopez Jose Manuel
Published: July 2023

The present work was performed to evaluate the levels of phytochemical constituents and the antioxidant and antibacterial properties of marine red seaweed (Kappaphycus alvarezii) and seagrass (Cymodocea serrulata). Quantitative phytochemical analysis, antioxidant activity and antimicrobial activity against five potential pathogenic bacteria was investigated. In each case, we found the presence of flavonoids, tannins, phenolic compounds, glycosides, steroids, carbohydrates and ashes. Alkaloids were only found in K. alvarezii, though they were not found in C. serrulata. The antimicrobial properties of both K. alvarezii and C. serrulata chloroform extracts were found to be antagonistically effective against the Gram-positive bacteria Bacillus subtilis and the Gram-negative bacteria Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi and Klebsiella pneumoniae. GC-MS analysis revealed the presence of 94 bioactive compounds in K. alvarezii and 104 bioactive compounds in C. serrulata, including phenol, decane, dodecane, hexadecane, vanillin, heptadecane, diphenylamine, benzophenone, octadecanoic acid, dotriaconate, benzene, phytol, butanoic acid and 2-hydroxyl-ethyl ether, which all played important roles in antioxidant and antibacterial activities. Thus, in view of the results, both K. alvarezii and C. serrulata could be considered to be sources of ingredients with appreciable nutritional and medicinal value.
Revisiting Vrancea (Romania) Intermediate-Depth Seismicity: Some Statistical Characteristics and Seismic Quiescence Testing
Authors: Enescu Bogdan , Ghita Cristian , Moldovan Iren-Adelina , Radulian Mircea
Published: July 2023

Background: The intermediate-depth seismicity in the Vrancea region (Romania) is characterized by localized and persistent earthquake activity that culminates about two or three times in a century with the occurrence of a large event (M ≥ 6.5). Here we have revisited some important seismicity characteristics, using earthquake catalog data spanning two different time periods: 1960–1999 and 2005–2013. Methods: we have determined the b-value of the frequency-magnitude distribution of earthquakes, using a maximum likelihood procedure, and estimated the parameter β to quantify anomalous seismicity rate decreases and increases. Results: by using data from the first period, we have confirmed the existence of a decreased b-value in the deepest part of the seismogenic zone; by using data from the second period, we have statistically confirmed the seismic quiescence that preceded the occurrence of the 1977 M7.4 Vrancea earthquake. Conclusions: the decreased b-value has been interpreted either in terms of an increased lithostatic stress with depth or as an indicator of the depth range where the next major Vrancea earthquake may occur. The time variation of the seismicity parameter β may reveal anomalous seismic quiescence and increased earthquake rates that may precede the occurrence of large earthquakes.
Effects of Acupressure on Maternal and Neonatal Obstetric Outcomes during Labor: Study Protocol
Authors: Carmona-Rodríguez Miguel Ángel , Martínez-Flores Salvador , Morilla-Romero-de-la-Osa Rubén , Luque-Oliveros Manuel
Published: July 2023

Background. A Cochrane review found that there is insufficient evidence to determine the effectiveness of acupressure for pain relief. One of the problems detected is the methodological variability reported. Objective. To assess the impact of the application of acupressure on obstetric and neonatal outcomes of labor, pain experience, and mother’s satisfaction with the experience. Method. Design of a protocol to carry out a two-arm multicenter single-blinded randomized controlled trial. Intervention (pressure on LI4 of the left hand, B6 of the left leg, GB21 of the left shoulder, and then the same sequence on the right side) and placebo (application of the technique on points not identified by acupuncture as key points) will be performed by a single researcher. Results. The recruitment began in April 2021 and, to date, there has been the participation of 40 women, divided into 17 included in the experimental group and 23 in the control. Communication of future results will be made in accordance with the CONSORT checklist. Conclusions. The designed protocol could methodologically improve some aspects of previous studies while maintaining adequate statistical power. The effectiveness of acupressure for one or more outcomes proposed (time and pain in labor) could support the inclusion of a new therapeutic tool in the clinical practice of midwives that would allow them to assist pregnant women, improving their experience both physically and psycho-emotionally
The Quality of Blood Donation Services and Its Association with Blood Donors’ Trust and Loyalty at Makkah Blood Donation Centers in Saudi Arabia: A Cross-Sectional Study
Authors: Kabrah Saeed M. , Abuzerr Samer , Almaghrabi Ruba Omar , Alserihi Raed , Felimban Raed I. , Mujalli Abdulrahman , Aslam Akhmed , Refaat Bassem , Halawani Amr J. , Alzhrani Adel A. , AlMoteri Naif Samran , Abusaadh Fauziah Fawzi , Bulkhi Rasha A.
Published: July 2023

The current cross-sectional study was conducted to determine the quality of blood donation services and its association with blood donors’ trust and loyalty at Makkah blood donation centers in Saudi Arabia. A total of 373 healthy blood donors aged ≥18 years who visited blood donation centers in Makkah, Saudi Arabia, between 1st and 28th February 2023 were recruited using a census sampling method. A pre-tested and validated Arabic language questionnaire was employed. The study survey included a checklist of sociodemographic variables (seven items), as well as seven-point Likert-scale questions on the quality of blood donation services (21 items), questions to assess the participant’s level of trust in blood donation centers (4 items), and questions to evaluate the level of loyalty to blood donations (4 items). SPSS (version 24) was used for data analysis. A total of 373 blood donors were included in this study. Of them, 240 (64.3%) were males and 133 (35.7%) were females. The vast majority of the study participants, 330 (88.5%), had a high educational level. The overall average agreement score for the quality of blood donation services was 71.7%. Furthermore, the overall average item agreement score for trust in blood donation centers and places was 83.0%, while the overall average item agreement score for loyalty to blood donation was 72.1%. Moreover, after adjustment for potential confounding factors, high levels of quality in blood donation services were associated with high levels of trust and loyalty among the blood donors (OR: 1.518, CI 95%: 0.321–0.864 and OR: 2.466, CI 95%: 0.285–0.763, respectively) (p-value < 0.05 for all). The overall quality of, trust in, and loyalty to blood donation services were 71.7%, 83.0%, and 72.1%, respectively. In addition, high levels of quality in blood donation services could improve blood donors’ trust and loyalty levels at Makkah blood donation centers in Saudi Arabia.
Reduction in the Incidence Density of Pressure Injuries in Intensive Care Units after Advance Preventive Protocols
Authors: Lien Ru-Yu , Wang Chien-Ying , Hung Shih-Hsin , Lu Shu-Fen , Yang Wen-Ju , Chin Shu-I , Chiang Dung-Hung , Lin Hui-Chen , Cheng Chun-Gu , Cheng Chun-An
Published: July 2023

(1) Background: Patients who are critically ill or undergo major surgery are admitted to intensive care units (ICUs). Prolonged immobilization is the most likely cause of pressure injuries (PrIs) in the ICU. Previous studies of Western populations found that effective protocols could reduce the incidence of PrIs, and the efficacy of systemic targeted intervention protocols in preventing PrIs in the Chinese population needs to be surveyed. (2) Methods: We reviewed cases of PrIs in the ICUs of Taipei Veterans General Hospital from 2014 to 2019. The ICU nurses at the hospital began to implement targeted interventions in January 2017. The incidence density of PrIs was calculated by dividing the number of PrIs by person days of hospitalizations in the pre-bundle (2014–2016) and post-bundle (2017–2019) stages. Poisson regression was performed to compare the trend of incidence densities. (3) Results: The incidence density of PrIs was 9.37/1000 person days during the pre-bundle stage and 1.85/1000 person days during the post-bundle stage (p < 0.001). The relative risk (RR) was 0.197 (95% confidence interval: 0.149–0.26). The incidence densities of iatrogenic PrIs and non-iatrogenic PrIs decreased as the RRs decreased. (4) Conclusions: Targeted interventions could significantly reduce the incidence of PrIs. Healthcare providers must follow the bundle care protocol for PrI prevention to improve the quality of healthcare and promote patient health.
Coregulators Reside within Drosophila Ecdysone-Inducible Loci before and after Ecdysone Treatment
Authors: Krasnov Aleksey N. , Evdokimova Aleksandra A. , Mazina Marina Yu , Erokhin Maksim , Chetverina Darya , Vorobyeva Nadezhda E.
Published: July 2023

Ecdysone signaling in Drosophila remains a popular model for investigating the mechanisms of steroid action in eukaryotes. The ecdysone receptor EcR can effectively bind ecdysone-response elements with or without the presence of a hormone. For years, EcR enhancers were thought to respond to ecdysone via recruiting coactivator complexes, which replace corepressors and stimulate transcription. However, the exact mechanism of transcription activation by ecdysone remains unclear. Here, we present experimental data on 11 various coregulators at ecdysone-responsive loci of Drosophila S2 cells. We describe the regulatory elements where coregulators reside within these loci and assess changes in their binding levels following 20-hydroxyecdysone treatment. In the current study, we detected the presence of some coregulators at the TSSs (active and inactive) and boundaries marked with CP190 rather than enhancers of the ecdysone-responsive loci where EcR binds. We observed minor changes in the coregulators’ binding level. Most were present at inducible loci before and after 20-hydroxyecdysone treatment. Our findings suggest that: (1) coregulators can activate a particular TSS operating from some distal region (which could be an enhancer, boundary regulatory region, or inactive TSS); (2) coregulators are not recruited after 20-hydroxyecdysone treatment to the responsive loci; rather, their functional activity changes (shown as an increase in H3K27 acetylation marks generated by CBP/p300/Nejire acetyltransferase). Taken together, our findings imply that the 20-hydroxyecdysone signal enhances the functional activity of coregulators rather than promoting their binding to regulatory regions during the ecdysone response.