bluuebunny/crossref_metadata_2025 huggingface dataset:
Decline in Vaccine-Type Streptococcus pneumoniae Serotypes Following Pneumococcal Conjugate Vaccine Introduction in Madagascar
Authors: Raboba Julia L , Rahajamanana Vonintsoa L , Andriatahirintsoa Emilson P R , Razafindrakoto Ainamalala C , Andrianarivelo Andry M , Nimpa Mengouo Marcellin , Vuo Masembe Yolande , Weldegebriel Goitom G , de Gouveia Linda , Mwenda Jason M , Robinson Annick L
Published: April 2021

Background

The 10-valent conjugate vaccine (PCV10) was introduced into the Extended Program on Immunization in Madagascar. We assessed the impact of PCV10 on the targeted pneumococcal serotypes among children < 5 years of age at Centre Hospitalier Universitaire Mère Enfant Tsaralalàna.

Method

Between 2012 and December 2018, cerebrospinal fluid (CSF) samples were collected and tested for S. pneumoniae by culture, and antigen tests. The Sentinel Site Laboratory (SSL) referred available CSF samples to the Regional Reference Laboratory (RRL) for real-time polymerase chain reaction confirmatory testing and serotyping.

Results

In total, 3616 CSF specimens were collected. The SSL referred 2716 to the RRL; 125 were positive for S. pneumoniae. At the RRL, 115 samples that tested positive for S. pneumoniae were serotyped; PCV10 serotypes accounted for 20%. Compared to the pre-PCV period, the proportion of S. pneumoniae detected declined from 22% to 6.6%, (P < .05), the proportion of PCV10 serotypes as the cause of pneumococcal meningitis cases declined by 26% following vaccine introduction.

Conclusions

In our findings, PCV10 introduction resulted in a decline of meningitis caused by S. pneumoniae and PCV10 vaccine serotypes.
In vitroactivity of imipenem/relebactam, meropenem/vaborbactam, ceftazidime/avibactam, cefepime/zidebactam and other novel antibiotics against imipenem-non-susceptible Gram-negative bacilli from Taiwan
Authors: Kuo Shu-Chen , Wang Yung-Chih , Tan Mei-Chen , Huang Wei-Cheng , Shiau Yih-Ru , Wang Hui-Ying , Lai Jui-Fen , Huang I-Wen , Lauderdale Tsai-Ling
Published: April 2021

Objectives

To investigate the susceptibility of imipenem-non-susceptible Escherichia coli (INS-EC), Klebsiella pneumoniae (INS-KP), Acinetobacter baumannii (INS-AB) and Pseudomonas aeruginosa (INS-PA) to novel antibiotics.

Methods

MICs were determined using the broth microdilution method. Carbapenemase and ESBL phenotypic testing and PCR for genes encoding ESBLs, AmpCs and carbapenemases were performed.

Results

Zidebactam, avibactam and relebactam increased the respective susceptibility rates to cefepime, ceftazidime and imipenem of 17 INS-EC by 58.8%, 58.8% and 70.6%, of 163 INS-KP by 77.9%, 88.3% and 76.1% and of 81 INS-PA by 45.7%, 38.3% and 85.2%, respectively. Vaborbactam increased the meropenem susceptibility of INS-EC by 41.2% and of INS-KP by 54%. Combinations of β-lactams and novel β-lactamase inhibitors or β-lactam enhancers (BLI-BLE) were inactive against 136 INS-AB. In 58 INS-EC and INS-KP with exclusively blaKPC-like genes, zidebactam, avibactam, relebactam and vaborbactam increased the susceptibility of the partner β-lactams by 100%, 96.6%, 84.5% and 75.9%, respectively. In the presence of avibactam, ceftazidime was active in an additional 85% of 20 INS-EC and INS-KP with exclusively blaOXA-48-like genes while with zidebactam, cefepime was active in an additional 75%. INS-EC and INS-KP with MBL genes were susceptible only to cefepime/zidebactam. The β-lactam/BLI-BLE combinations were active against INS-EC and INS-KP without detectable carbapenemases. For INS-EC, INS-KP and INS-AB, tigecycline was more active than omadacycline and eravacycline but eravacycline had a lower MIC distribution. Lascufloxacin and delafloxacin were active in <35% of these INS isolates.

Conclusions

β-Lactam/BLI-BLE combinations were active in a higher proportion of INS-EC, INS-KP and INS-PA. The susceptibility of novel fluoroquinolones and tetracyclines was not superior to that of old ones.
Low Bottleneck Detection in Long-Lived Species Despite Lost Genetic Diversity: A Case Study of Tuatara and Eastern Massasauga Rattlesnakes
Authors: Bradke Danielle R , Altobelli Joseph T , Russell Amy L , Jaeger Collin P , Moore Jennifer A
Published: April 2021

Population bottlenecks can reduce genetic diversity and may lead to inbreeding depression. However, some studies have provided evidence that long lifespans buffer negative genetic effects of bottlenecks. Others have cautioned that longevity might merely mask the effects of genetic drift, which will still affect long-term population viability. We used microsatellite data from actual populations of tuatara (Sphenodon punctatus) and eastern massasaugas (Sistrurus catenatus) as a starting point for simulated population declines to evaluate the performance of bottleneck tests under a range of scenarios. We quantified losses in genetic diversity for each scenario and assessed the power of commonly used tests (i.e., M-ratio, heterozygosity excess, and mode-shift) to detect known bottlenecks in these moderate- to long-lived species. Declines in genetic diversity were greater in bottlenecks simulated for eastern massasaugas, the shorter-lived species, and mode-shift and heterozygosity excess tests were more sensitive to population declines in this species. Conversely, M-ratio tests were more sensitive to bottlenecks simulated in tuatara. Despite dramatic simulated population declines, heterozygosity excess and mode-shift tests often failed to detect bottlenecks in both species, even when large losses in genetic diversity had occurred (both allelic diversity and heterozygosity). While not eliminating type II error, M-ratio tests generally performed best and were most reliable when a critical value (Mc) of 0.68 was used. However, in tuatara simulations, M-ratio tests had high rates of type I error when Mc was calculated assuming θ = 10. Our results suggest that reliance on these tests could lead to misguided species management decisions.
Keeping institutional logics in arm’s length: emerging of rogue practices in a gray zone of everyday work life in healthcare
Authors: Gürses Serdal , Danışman Ali
Published: April 2021

We set out to explore the practice-level cognitive structures and associated practices characterizing the daily routine work of physicians by conducting a qualitative study in the Turkish healthcare field, in which a recent government-led healthcare reform was implemented causing logic multiplicity. Contrary to the accumulated knowledge in institutional logics literature, a bulk of which suggests that actors craft and enact various practices in managing plural and at times conflicting institutional templates strictly within the confines of higher order societal logics, this study shows that while ground level actors may not exercise complete freedom and maneuverability in relation to pre-established social structures, they do incorporate unconventional schemas of action; namely rogue practices, into their embodied practical activity, which over time become routinized in their day-to-day work lives. Unraveling the dynamics of micro-level practices of highly professionalized ground level actors as they pertain to atypical logical orientations substantially advances our understanding of the unknown or unseen side of how and under which conditions certain or various combinations of institutional logics are employed during day-to-day activities.
A Model-Assisted Approach for Finding Coding Errors in Manual Coding of Open-Ended Questions
Authors: He Zhoushanyue , Schonlau Matthias
Published: April 2021

Text answers to open-ended questions are typically manually coded into one of several codes. Usually, a random subset of text answers is double-coded to assess intercoder reliability, but most of the data remain single-coded. Any disagreement between the two coders points to an error by one of the coders. When the budget allows double coding additional text answers, we propose employing statistical learning models to predict which single-coded answers have a high risk of a coding error. Specifically, we train a model on the double-coded random subset and predict the probability that the single-coded codes are correct. Then, text answers with the highest risk are double-coded to verify. In experiments with three data sets, we found that this method identifies two to three times as many coding errors in the additional text answers as compared to random guessing, on average. We conclude that this method is preferred if the budget permits additional double-coding. When there are a lot of intercoder disagreements, the benefit can be substantial.
Mild radial variations of the stellar IMF in the bulge of M31
Authors: La Barbera F , Vazdekis A , Ferreras I , Pasquali A
Published: April 2021

Using new, homogeneous, long-slit spectroscopy in the wavelength range from ∼0.35 to $\sim 1 \, \mu$m, we study radial gradients of optical and near-infrared (NIR) initial mass function (IMF)-sensitive features along the major axis of the bulge of M31, out to a galactocentric distance of ∼200 arcsec (∼800 pc). Based on state-of-the-art stellar population synthesis models with varying Na abundance ratio, we fit a number of spectral indices, from different chemical species (including TiO’s, Ca, and Na indices), to constrain the low-mass (≲0.5 M⊙) end slope (i.e. the fraction of low-mass stars) of the stellar IMF, as a function of galactocentric distance. Outside a radial distance of ∼10 arcsec, we infer an IMF similar to a Milky Way-like distribution, while at small galactocentric distances, an IMF radial gradient is detected, with a mildly bottom-heavy IMF in the few inner arcsec. We are able to fit Na features (both NaD and $\rm Na\,{\small I}8190$), without requiring extremely high Na abundance ratios. $\rm [Na/Fe]$ is ∼0.4 dex for most of the bulge, rising up to ∼0.6 dex in the innermost radial bins. Our results imply an overall, luminosity-weighted, IMF and mass-to-light ratio for the M31 bulge, consistent with those for a Milky Way-like distribution, in contrast to results obtained, in general, for most massive early-type galaxies.
Detection of an energetic flare from the M5V secondary star in the Polar MQ Dra
Authors: Ramsay Gavin , Hakala Pasi , Wood Matt A
Published: April 2021

MQ Dra is a strongly magnetic Cataclysmic Variable whose white dwarf accretes material from its secondary star through a stellar wind at a low rate. TESS observations were made of MQ Dra in four sectors in Cycle 2 and show a short duration, high energy flare (∼1035 erg) which has a profile characteristic of a flare from the M5V secondary star. This is one of the few occasions where an energetic flare has been seen from a Polar. We find no evidence that the flare caused a change in the light curve following the event and consider whether a coronal mass ejection was associated with the flare. We compare the frequency of energetic flares from the secondary star in MQ Dra with M dwarf stars and discuss the overall flare rate of stars with rotation periods shorter than 0.2 d and how such fast rotators can generate magnetic fields with low differential rotation rates.
Exploring higher order images with Fe Kα-lines from relativistic discs: black hole spin determination and bias
Authors: Falanga M , Bakala P , Placa R La , De Falco V , De Rosa A , Stella L
Published: April 2021

We study the contributions to the relativistic Fe K α line profile from higher order images (HOIs) produced by strongly deflected rays from the disc which cross the plunging region, located between the innermost stable circular orbit (ISCO) radius and the event horizon of a Kerr black hole. We investigate the characteristics features imprinted by the HOIs in the line profile for different black hole spins, disc emissivity laws, and inclinations. We find that they extend from the red wing of the profile up to energies slightly lower than those of the blue peak, adding ∼0.4–1.3 per cent to the total line flux. The contribution to the specific flux is often in the ∼1 to 7 per cent range, with the highest values attained for low and negative spin (a ≲ 0.3) black holes surrounded by intermediate inclination angle (i ∼ 40°) discs. We simulate future observations of a black hole X-ray binary system with the Large Area Detector of the planned X-ray astronomy enhanced X-ray Timing and Polarimetry Mission (eXTP) and find that the Fe Kα line profiles of systems accreting at ≲1 per cent the Eddington rate are affected by the HOI features for a range of parameters. This would provide evidence of the extreme gravitational lensing of HOI rays. Our simulations show also that not accounting for HOI contributions to the Fe Kα line profile may systematically bias measurements of the black hole spin parameter towards values higher by up to ∼0.3 than the inputted ones.
Apparent radio transients mapping the near-Earth plasma environment
Authors: Kuiack Mark J , Wijers Ralph A M J , Shulevski Aleksandar , Rowlinson Antonia
Published: April 2021

We report the discovery of bright, fast, radio flashes lasting tens of seconds with the AARTFAAC high-cadence all-sky survey at 60 MHz. The vast majority of these coincide with known, bright radio sources that brighten by factors of up to 100 during such an event. We attribute them to magnification events induced by plasma near the Earth, most likely in the densest parts of the ionosphere. They can occur both in relative isolation, during otherwise quiescent ionospheric conditions, and in large clusters during more turbulent ionospheric conditions. Using a toy model, we show that the likely origin of the more extreme (up to a factor of 100 or so) magnification events likely originate in the region of peak electron density in the ionosphere, at an altitude of 300–400 km. Distinguishing these events from genuine astrophysical transients is imperative for future surveys searching for low frequency radio transient at time-scales below a minute.
Chemical composition of stars with massive planets
Authors: Mishenina T , Basak N , Adibekyan V , Soubiran C , Kovtyukh V
Published: April 2021

Stellar parameters of 25 planet-hosting stars and abundances of Li, C, O, Na, Mg, Al, S, Si, Ca, Sc, Ti, V, Cr, Mn, Fe, Ni, Zn, Y, Zr, Ba, Ce, Pr, Nd, Sm, and Eu were studied based on homogeneous high-resolution spectra and uniform techniques. The iron abundance [Fe/H] and key elements (Li, C, O, Mg, Si) indicative of the planet formation, as well as the dependences of [El/Fe] on Tcond, were analysed. The iron abundances determined in our sample stars with detected massive planets range within –0.3 < [Fe/H] < 0.4. The behaviour of [C/Fe], [O/Fe], [Mg/Fe], and [Si/Fe] relative to [Fe/H] is consistent with the Galactic Chemical Evolution trends. The mean values of C/O and [C/O] are $\langle$C/O$\rangle$ = 0.48 ± 0.07 and $\langle$[C/O]$\rangle$ = –0.07 ± 0.07, which are slightly lower than solar ones. The Mg/Si ratios range from 0.83 to 0.95 for four stars in our sample and from 1.0 to 1.86 for the remaining 21 stars. Various slopes of [El/Fe] versus Tcond were found. The dependences of the planetary mass on metallicity, the lithium abundance, the C/O and Mg/Si ratios, and also on the [El/Fe]–Tcond slopes were considered.
Properties of the ionized CGM and IGM: tests for galaxy formation models from the Sunyaev–Zel’dovich effect
Authors: Lim S H , Barnes D , Vogelsberger M , Mo H J , Nelson D , Pillepich A , Dolag K , Marinacci F
Published: April 2021

We present a comparison of the physical properties of the ionized gas in the circumgalactic medium and intergalactic medium (IGM) at z ∼ 0 between observations and four cosmological hydrodynamical simulations: Illustris, TNG300 of the IllustrisTNG project, EAGLE, and one of the Magneticum simulations. For the observational data, we use the gas properties that are inferred from cross-correlating the Sunyaev–Zel’dovich effect (SZE) from the Planck CMB maps with haloes and large-scale structure. Both the observational and simulation results indicate that the integrated gas pressure in haloes deviates from the self-similar case, showing that feedback impacts haloes with $M_{500}\sim 10^{12\!-\!13}\, {\rm M_\odot }$. The simulations predict that more than half the baryons are displaced from haloes, while the gas fraction inferred from our observational data roughly equals the cosmic baryon fraction throughout the $M_{500}\sim 10^{12\!-\!14.5}\, {\rm M_\odot }$ halo mass range. All simulations tested here predict that the mean gas temperature in haloes is about the virial temperature, while that inferred from the SZE is up to one order of magnitude lower than that from the simulations (and also from X-ray observations). While a remarkable agreement is found for the average properties of the IGM between the observation and some simulations, we show that their dependence on the large-scale tidal field can break the degeneracy between models that show similar predictions otherwise. Finally, we show that the gas pressure and the electron density profiles from simulations are not well described by a generalized NFW profile. Instead, we present a new model with a mass-dependent shape that fits the profiles accurately.
Cavitation fatigue in conifers: a study on eight European species
Authors: Feng Feng , Losso Adriano , Tyree Melvin , Zhang Shuoxin , Mayr Stefan
Published: April 2021

After drought-induced embolism and repair, tree xylem may be weakened against future drought events (cavitation fatigue). As there are few data on cavitation fatigue in conifers available, we quantified vulnerability curves (VCs) after embolism/repair cycles on eight European conifer species. We induced 50% and 100% loss of conductivity (LC) with a cavitron, and analyzed VCs. Embolism repair was obtained by vacuum infiltration. All species demonstrated complete embolism repair and a lack of any cavitation fatigue after 50% LC . After 100% LC, European larch (Larix decidua), stone pine (Pinus cembra), Norway spruce (Picea abies), and silver fir (Abies alba) remained unaffected, while mountain pine (Pinus mugo), yew (Taxus baccata), and common juniper (Juniperus communis) exhibited 0.4–0.9 MPa higher vulnerability to embolism. A small cavitation fatigue observed in Scots pine (Pinus sylvestris) was probably biased by incomplete embolism repair, as indicated by a correlation of vulnerability shifts and conductivity restoration. Our data demonstrate that cavitation fatigue in conifers is species-specific and depends on the intensity of preceding LC. The lack of fatigue effects after moderate LC, and relevant effects in only three species after high LC, indicate that conifers are relatively resistant against cavitation fatigue. This is remarkable considering the complex and delicate conifer pit architecture and may be important considering climate change projections.
Maize unstable factor for orange1 is essential for endosperm development and carbohydrate accumulation
Authors: Chatterjee Debamalya , Wittmeyer Kameron , Lee Tzuu-fen , Cui Jin , Yennawar Neela H , Yennawar Hemant P , Meyers Blake C , Chopra Surinder
Published: April 2021

Maize (Zea mays L.) Ufo1-1 is a spontaneous dominant mutation of the unstable factor for orange1 (ufo1). We recently cloned ufo1, which is a Poaceae-specific gene highly expressed during seed development in maize. Here, we have characterized Ufo1-1 and a loss-of-function Ds insertion allele (ufo1-Dsg) to decipher the role of ufo1 in maize. We found that both ufo1 mutant alleles impact sugars and hormones, and have defects in the basal endosperm transfer layer (BETL) and adjacent cell types. The Ufo1-1 BETL had reduced cell elongation and cell wall ingrowth, resulting in cuboidal shaped transfer cells. In contrast, the ufo1-Dsg BETL cells showed a reduced overall size with abnormal wall ingrowth. Expression analysis identified the impact of ufo1 on several genes essential for BETL development. The overexpression of Ufo1-1 in various tissues leads to ectopic phenotypes, including abnormal cell organization and stomata subsidiary cell defects. Interestingly, pericarp and leaf transcriptomes also showed that as compared with wild type, Ufo1-1 had ectopic expression of endosperm development-specific genes. This study shows that Ufo1-1 impacts the expression patterns of a wide range of genes involved in various developmental processes.
The sFlt-1 to PlGF ratio in pregnant women with rheumatoid arthritis: impact of disease activity and sulfasalazine use
Authors: Neuman Rugina I , Smeele Hieronymus T W , Jan Danser A H , Dolhain Radboud J E M , Visser Willy
Published: April 2021

Objectives

An elevated sFlt-1/PlGF ratio has been validated as a significant predictor of preeclampsia, but has not been established in women with RA. We explored whether the sFlt-1/PlGFratio could be altered due to disease activity in RA, and could be applied in this population to predict preeclampsia. Since SSZ has been suggested to improve the angiogenic imbalance in preeclampsia, we also aimed to examine whether SSZ could affect sFlt-1 or PlGF levels.

Methods

Making use of a nationwide, observational, prospective cohort study on pregnant women with RA, sFlt-1 and PlGF were measured in the third trimester. A total of 221 women, aged 21–42 years, were included, with a median gestational age of 30 + 3 weeks.

Results

No differences in sFlt-1 or PlGF were observed between women with high, intermediate or low disease activity (P = 0.07 and P = 0.41), whereas sFlt-1 and PlGF did not correlate with DAS28-CRP score (r = −0.01 and r = −0.05, respectively). Four (2%) women with a sFlt-1/PlGF ratio ≤38 developed preeclampsia in comparison to three (43%) women with a ratio > 38, corresponding to a negative predictive value of 98.1%. SSZ users (n = 57) did not show altered levels of sFlt-1 or PlGF in comparison to non-SSZ users (n = 164, P = 0.91 and P = 0.11).

Conclusion

Our study shows that in pregnant women with RA, the sFlt-1/PlGF ratio is not altered due to disease activity and a cut-off ≤38 can be used to exclude preeclampsia. Additionally, SSZ use did not affect sFlt-1 or PlGF levels in this population.
A novel fluorescence optical imaging scoring system for hand synovitis in rheumatoid arthritis—validity and agreement with ultrasound
Authors: Ammitzbøll-Danielsen Mads , Glinatsi Daniel , Terslev Lene , Østergaard Mikkel
Published: April 2021

Objectives

To develop and validate a new semiquantitative fluorescence optical imaging (FOI) scoring system—the FOI Enhancement-Generated RA Score (FOIE-GRAS) for synovitis assessment in the hand.

Methods

The development of FOIE-GRAS was based on consensus of four experts in musculoskeletal imaging. Forty-six RA patients, eligible for treatment intensification and with ≥1 clinically swollen joints in the hands, and 11 healthy controls were included. FOI, ultrasound and clinical assessment of both hands were obtained at baseline and for RA patients after 3 and 6 months’ follow-up. Twenty RA patients had an FOI rescan after 4 h. Synovitis was scored using FOIE-GRAS and the OMERACT ultrasound synovitis scoring system. All FOI images were scored by two readers. Inter-scan, inter- and intra-reader reliability were determined. Furthermore, FOIE-GRAS agreement with ultrasound and responsiveness was assessed.

Results

FOIE-GRAS synovitis was defined as early enhancement, and scores were based on the degree of coverage of the specific joint region after 3 s (0–3). Inter-scan, intra- and inter-reader intraclass correlations coefficients (ICC) were good to excellent for all baseline scores (0.76–0.98) and moderate to good for change (0.65–76).

The FOIE-GRAS had moderate agreement with ultrasound (ICC 0.30–0.54) for total score, a good standardized response mean (>0.80), and moderate correlation with clinical joint assessment and DAS28-CRP. The median (interquartile range) reading time per FOI examination was 133 (109, 161) s. Scores were significantly lower in controls [1 (0, 4)] than RA patients [11 (6, 19)].

Conclusion

The FOIE-GRAS offers a feasible and reliable assessment of synovitis in RA, with a moderate correlation with ultrasound and DAS28-CRP, and good responsiveness.
Do tender joints in active psoriatic arthritis reflect inflammation assessed by ultrasound and magnetic resonance imaging?
Authors: Felbo Sara Kamp , Wiell Charlotte , Østergaard Mikkel , Poggenborg René Panduro , Bøyesen Pernille , Hammer Hilde Berner , Boonen Annelies , Pedersen Susanne Juhl , Sørensen Inge Juul , Madsen Ole Rintek , Slot Ole , Møller Jakob Møllenbach , Szkudlarek Marcin , Terslev Lene
Published: April 2021

Objective

To investigate the association between clinical joint tenderness and intra- and periarticular inflammation as assessed by ultrasound and MRI in patients with active PsA and to explore if the associations differ according to patient-reported outcomes (PROs) and structural damage.

Methods

Forty-one patients with active PsA and hand involvement had 76/78 joints examined for swelling/tenderness and ultrasound and MRI of 24 and 12 finger joints, respectively. Synovitis, tenosynovitis, periarticular inflammation and erosions were assessed using OMERACT definitions and scoring systems. Correlation between imaging inflammation sum-scores (intra-and periarticular) and tender/swollen joint counts were calculated using Spearman’s rho, agreement at joint level was examined using prevalence and bias adjusted kappa (PABAK). Subgroup analyses explored the influence of PROs and radiographic erosive disease on these associations.

Results

No significant correlations were found between tender or swollen joint counts and imaging inflammation sum-scores (rho = −0.31–0.38). In patients with higher level of overall pain, disability and lower self-reported mental health, a tendency towards negative correlations were found. At joint level, intra- and periarticular imaging inflammatory lesions had slight agreement with joint tenderness (PABAK = 0.02–0.19) and slight to moderate with swelling (PABAK = 0.16–0.54). For tender joints, agreement with imaging inflammation was even weaker in patients with either high overall pain scores, high disability scores, and/or non-erosive disease.

Conclusion

Joint tenderness had low association with imaging signs of inflammation in PsA patients, particularly in patients with high self-reported pain, disability and low mental health, indicating that tenderness is influenced by other parameters than local inflammation.
Prognostic Accuracy of DSM-5 Attenuated Psychosis Syndrome in Adolescents: Prospective Real-World 5-Year Cohort Study
Authors: Mensi Martina Maria , Molteni Silvia , Iorio Melanie , Filosi Eleonora , Ballante Elena , Balottin Umberto , Fusar-Poli Paolo , Borgatti Renato
Published: April 2021

There is limited research in adolescents at risk for psychosis. The new Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition attenuated psychosis syndrome (DSM-5 APS) criteria have not been validated in this group. We conducted a RECORD-compliant, real-world, prospective, 5-year cohort study addressing clinical profile, transition to psychosis, and prognostic accuracy of DSM-5 APS in help-seeking inpatient/outpatient adolescents accessing Children and Adolescent Neuropsychiatric services at IRCCS Mondino Foundation (Pavia, Lombardy, Italy) between 2012 and 2019. About 243 adolescents (31 early-onset psychosis [EOP]; 110 meeting DSM-5 APS criteria, DSM-5 APS; 102 not meeting psychotic or DSM-5 APS criteria, non-APS) were included. At baseline, DSM-5 APS adolescents (aged 15.4 ± 1.6) had on average 2.3 comorbid disorders (higher than EOP/non-APS, P < .001). DSM-5 APS adolescents had an intermediate psychopathological profile between non-APS/EOP (P < .001) and worsen Clinical Global Impression-Severity than non-APS (P < .001). DSM-5 APS functioning was intermediate between non-APS and EOP. 39.1% of DSM-5 APS were treated with psychotropic drugs (average = 64 days); 53.6% received psychotherapy. Follow-up of DSM-5 APS and non-APS groups lasted 33 and 26 months, respectively (median). The cumulative risk of transition at 1–5 years was 13%, 17%, 24.2%, 26.8%, and 26.8% in the DSM-5 APS group, 0%, 0%, 3.2%, 3.2%, and 3.2% in the non-APS group. The 5-year prognostic accuracy of the DSM-5 APS in adolescent was adequate (area under the curve = 0.77; Harrell’s C = 0.736, 95%CI 0.697–0.775), with high sensitivity (91.3%) and suboptimal specificity (63.2%). The DSM-5 APS diagnosis can be used to detect help-seeking adolescents at risk of psychosis and predict their long-term outcomes. Future research should consolidate these findings.
(De)criminalization of Survivors of Domestic Minor Sex Trafficking: A Social Work Call to Action
Authors: Gezinski Lindsay B
Published: April 2021

Survivors of domestic minor sex trafficking (DMST) are failing to be identified and are frequently criminalized. Examining the linkages between DMST and the juvenile legal system is important, as many trafficked youths are only identified after entry into the child welfare or juvenile court system as a result of being arrested or detained on related delinquency charges. Due to legacies of structural violence, marginalized youths are reluctant to approach police officers for help. Therefore, social workers have an opportunity to identify, intervene, and advocate for trafficked youths. In this article, the author provides an overview of existing research related to the demographic profile of DMST, homelessness and survival sex, recruitment and entry, barriers to effective community response, and prevention and intervention strategies. This article represents a call to broaden our view of young people in the sex trade and supports the decriminalization of trafficked youths. Interventions and policies must be designed to fit their complex needs and experiences, resulting in empowerment, opportunity, and new beginnings.
Hydraulic segmentation does not protect stems from acute water loss during fire
Authors: Hoffmann William A , Rodrigues Amanda C , Uncles Nicholas , Rossi Lorenzo
Published: April 2021

The heat plume associated with fire has been hypothesized to cause sufficient water loss from trees to induce embolism and hydraulic failure. However, it is unclear whether the water transport path remains sufficiently intact during scorching or burning of foliage to sustain high water loss. We measured water uptake by branches of Magnolia grandiflora while exposing them to a range of fire intensities and examined factors influencing continued water uptake after fire. Burning caused a 22-fold mean increase in water uptake, with greatest rates of water loss observed at burn intensities that caused complete consumption of leaves. Such rapid uptake is possible only with steep gradients in water potential, which would likely result in substantial cavitation of xylem and loss of conductivity in intact stems. Water uptake continued after burning was complete and was greatest following burn intensities that killed leaves but did not consume them. This post-fire uptake was mostly driven by rehydration of the remaining tissues, rather than evaporation from the tissues. Our results indicate that the fire plume hypothesis can be expanded to include a wide range of burning conditions experienced by plants. High rates of water loss are sustained during burning, even when leaves are killed or completely consumed.
The performance of the recombinase polymerase amplification test for detecting Leishmania deoxyribonucleic acid from skin lesions of patients with clinical or epidemiological suspicion of cutaneous leishmaniasis
Authors: Mesa Luz Estella , Manrique Rubén , Robledo Sara M , Tabares Jessica , Pineda Tatiana , Muskus Carlos
Published: April 2021

Background

The diagnosis of cutaneous leishmaniasis (CL) is based on demonstration of the presence of the parasite in samples obtained from the lesions by direct examination (DE), culture or polymerase chain reaction (PCR)-based molecular tests. Recombinase polymerase amplification (RPA) represents an isothermal version of the conventional PCR (cPCR) technique, being ideal for detecting Leishmania DNA, especially in field conditions.

Methods

A prospective and cross-sectional study was conducted to evaluate the diagnostic performance of RPA in the health centres of rural endemic sites or the evaluation centre (EC) of 11 Colombian municipalities and in a reference centre (RC).

Results

Samples of 128 patients with suspected CL were included and processed for analysis by RPA vs DE in the EC and RPA vs DE and cPCR in the RC. The RPA performed at the EC was more sensitive (90.4% [95% confidence interval {CI} 81.9 to 95.7]) than the DE (42–67%) and the specificity was 72.7% (95% CI 57.2 to 85.0). Both the sensitivity and specificity increased to 100% when adjusting by the imperfect reference standard analysis method. In the RC, the sensitivity of RPA vs cPCR was 72% and the specificity was 69.8%, while the sensitivity of cPCR vs the DE test was 78.8% and the specificity was 81%.

Conclusions

The higher sensitivity and specificity shown by RPA in the EC, but also its ease and speed of use, justify performing RPA in the health centres of rural endemic sites. In addition, RPA eliminates the subjectivity inherent in the traditional DE.
Including fossils in phylogeny: a glimpse into the evolution of the superfamily Evanioidea (Hymenoptera: Apocrita) under tip-dating and the fossilized birth–death process
Authors: Jouault Corentin , Maréchal Arthur , Condamine Fabien L , Wang Bo , Nel André , Legendre Frédéric , Perrichot Vincent
Published: April 2021

Using a fossilized birth–death model, a new phylogeny of the superfamily Evanioidea (including ensign wasps, nightshade wasps and hatchet wasps) is proposed, with estimates of divergence times for its constitutive families and for corroborating the monophyly of Evanioidea. Additionally, our Bayesian analyses demonstrate the monophyly of †Anomopterellidae, †Othniodellithidae, †Andreneliidae, Aulacidae, Gasteruptiida and Evaniidae, whereas †Praeaulacidae and †Baissidae appear to be paraphyletic. Vectevania vetula and Hyptiogastrites electrinus are transferred to Aulacidae. We estimate the divergence time of Evanioidea to be in the Late Triassic (~203 Mya). Additionally, three new othniodellithid wasps are described and figured from mid-Cretaceous Burmese amber as the new genus Keratodellitha, with three new species: Keratodellitha anubis sp. nov., Keratodellitha basilisci sp. nov. and Keratodellitha kirin sp. nov. We also document a temporal shift in relative species richness between Ichneumonoidea and Evanioidea.
How social determinants of health affect COVID-19-related morbidity and mortality
Authors: Pecoraro Kathleen A.
Published: April 2021

The conditions under which people live and work and how those conditions affect health are known as social determinants of health. They are impacted by disparities of wealth, opportunity, and other societal resources. This article examines how these disparities have influenced morbidity and mortality in minority people of color during the COVID-19 pandemic.
Patient Involvement in Anesthesia Decision-making: A Qualitative Study of Knee Arthroplasty
Authors: Graff Veena , Clapp Justin T. , Heins Sarah J. , Chung Jamison J. , Muralidharan Madhavi , Fleisher Lee A. , Elkassabany Nabil M.
Published: April 2021

Background

Calls to better involve patients in decisions about anesthesia—e.g., through shared decision-making—are intensifying. However, several features of anesthesia consultation make it unclear how patients should participate in decisions. Evaluating the feasibility and desirability of carrying out shared decision-making in anesthesia requires better understanding of preoperative conversations. The objective of this qualitative study was to characterize how preoperative consultations for primary knee arthroplasty arrived at decisions about primary anesthesia.

Methods

This focused ethnography was performed at a U.S. academic medical center. The authors audio-recorded consultations of 36 primary knee arthroplasty patients with eight anesthesiologists. Patients and anesthesiologists also participated in semi-structured interviews. Consultation and interview transcripts were coded in an iterative process to develop an explanation of how anesthesiologists and patients made decisions about primary anesthesia.

Results

The authors found variation across accounts of anesthesiologists and patients as to whether the consultation was a collaborative decision-making scenario or simply meant to inform patients. Consultations displayed a number of decision-making patterns, from the anesthesiologist not disclosing options to the anesthesiologist strictly adhering to a position of equipoise; however, most consultations fell between these poles, with the anesthesiologist presenting options, recommending one, and persuading hesitant patients to accept it. Anesthesiologists made patients feel more comfortable with their proposed approach through extensive comparisons to more familiar experiences.

Conclusions

Anesthesia consultations are multifaceted encounters that serve several functions. In some cases, the involvement of patients in determining the anesthetic approach might not be the most important of these functions. Broad consideration should be given to both the applicability and feasibility of shared decision-making in anesthesia consultation. The potential benefits of interventions designed to enhance patient involvement in decision-making should be weighed against their potential to pull anesthesiologists’ attention away from important humanistic aspects of communication such as decreasing patients’ anxiety.

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Predicting fetal weight by three-dimensional limb volume ultrasound (AVol/TVol) and abdominal circumference
Authors: Kang Li , Wu Qing-Qing , Sun Li-Juan , Gao Feng-Yun , Wang Jing-Jing
Published: April 2021

Background:

Fetal weight is an important parameter to ensure maternal and child safety. The purpose of this study was to use three-dimensional (3D) limb volume ultrasound combined with fetal abdominal circumference (AC) measurement to establish a model to predict fetal weight and evaluate its efficiency.

Methods:

A total of 211 participants with single pregnancy (28–42 weeks) were selected between September 2017 and December 2018 in the Beijing Obstetrics and Gynecology Hospital of Capital Medical University. The upper arm (AVol)/thigh volume (TVol) of fetuses was measured by the 3D limb volume technique. Fetal AC was measured by two-dimensional ultrasound. Nine cases were excluded due to incomplete information or the interval between examination and delivery >7 days. The enrolled 202 participants were divided into a model group (134 cases, 70%) and a verification group (68 cases, 30%) by mechanical sampling method. The linear relationship between limb volume and fetal weight was evaluated using Pearson Chi-squared test. The prediction model formula was established by multivariate regression with data from the model group. Accuracy of the model formula was evaluated with verification group data and compared with traditional formulas (Hadlock, Lee2009, and INTERGROWTH-21st) by paired t-test and residual analysis. Receiver operating characteristic curves were generated to predict macrosomia.

Results:

AC, AVol, and TVol were linearly related to fetal weight. Pearson correlation coefficient was 0.866, 0.862, and 0.910, respectively. The prediction model based on AVol/TVol and AC was established as follows: Y = −481.965 + 12.194TVol + 15.358AVol + 67.998AC, R
2
adj = 0.868. The scatter plot showed that when birth weight fluctuated by 5% (i.e., 95% to 105%), the difference between the predicted fetal weight by the model and the actual weight was small. A paired t-test showed that there was no significant difference between the predicted fetal weight and the actual birth weight (t = −1.015, P = 0.314). Moreover, the residual analysis showed that the model formula's prediction efficiency was better than the traditional formulas with a mean residual of 35,360.170. The combined model of AVol/TVol and AC was superior to the Lee2009 and INTERGROWTH-21st formulas in the diagnosis of macrosomia. Its predictive sensitivity and specificity were 87.5% and 91.7%, respectively.

Conclusion:

Fetal weight prediction model established by semi-automatic 3D limb volume combined with AC is of high accuracy, sensitivity, and specificity. The prediction model formula shows higher predictive efficiency, especially for the diagnosis of macrosomia.

Trial Registration:

ClinicalTrials.gov, NCT03002246; https://clinicaltrials.gov/ct2/show/NCT03002246?recrs=e&cond=fetal&draw=8&rank=67.
Human immunodeficiency virus/acquired immunodeficiency syndrome prevalence, incidence, and mortality in China, 1990 to 2017: a secondary analysis of the Global Burden of Disease Study 2017 data
Authors: Liu Xue-Jiao , McGoogan Jennifer M. , Wu Zun-You
Published: April 2021

Background:

Despite almost two decades of well-funded and comprehensive response efforts by the Chinese Government, human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) remains a major problem in China. Yet, few studies have recently examined long-term trends in HIV/AIDS prevalence, incidence, and mortality at the national level. This study aimed to determine the prevalence, incidence, and mortality trends for HIV/AIDS over the past 28 years in China.

Methods:

We conducted a descriptive, epidemiological, secondary analysis of the Global Burden of Diseases, Injuries, and Risk Factors Study 2017 data. To evaluate trends in prevalence, incidence, and mortality over the study period from 1990 to 2017, we calculated values for annual percentage change (APC) and corresponding 95% confidence intervals (CIs) using joinpoint regression analysis.

Results:

A significant increase in HIV/AIDS prevalence was observed for 1990 to 2009 (APC: 10.7; 95% CI: 10.4, 11.0; P < 0.001), and then remained stable for 2009 to 2017 (APC: 0.7; 95% CI: −0.3, 1.7; P = 0.1). A significant increase in HIV incidence was also observed for 1990 to 2005 (APC: 13.0; 95% CI: 12.6, 13.4; P < 0.001), and then a significant decrease was detected for 2005 to 2017 (APC: −6.5; 95% CI: −7.0, −6.1; P < 0.001). A significant increase in AIDS-related mortality rate was detected for 1990 to 2004 (APC: 10.3; 95% CI: 9.3, 11.3; P < 0.001), followed by a period of stability for 2004 to 2013 (APC: 1.3; 95% CI: −0.7, 3.3; P = 0.2), and then another significant increase for 2013 to 2017 (APC: 15.3; 95% CI: 8.7, 22.2; P < 0.001).

Conclusions:

Although prevalence has stabilized and incidence has declined, AIDS-related mortality has risen sharply in recent years. These findings suggest more must be done to bring people into treatment earlier, retain them in treatment more effectively, actively seek to reenter them in treatment if they dropout, and improve the quality of treatment and care regimens.
Circ_0049447 acts as a tumor suppressor in gastric cancer through reducing proliferation, migration, invasion, and epithelial-mesenchymal transition
Authors: Tang Kai-Wen , Guo Zhe-Xu , Wu Zhong-Hua , Zhou Cen , Sun Jie , Wang Xin , Song Yong-Xi , Wang Zhen-Ning
Published: April 2021

Background:

Although increasing abnormal expression of circular RNAs (circRNAs) has been revealed in various cancers, there were a small number of studies about circRNAs in gastric cancer (GC). Here, we explored the expression and function of a novel circRNA, circ_0049447, in GC.

Methods:

A total of 80 GC tissues and non-tumorous tissues were collected from the First Affiliated Hospital of China Medical University. And all cells were cultured with 10% fetal bovine serum and incubated at 37°C and 5% CO2. The expression of circ_0049447 was quantified by real-time polymerase chain reaction. The biological function of circ_0049447 on proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) was evaluated by cell counting kit-8 (CCK-8), colony formation assay, transwell migration and invasion assay, and Western blotting. Luciferase report assay was used to verify the direct binding between circ_0049447 and predicted microRNA (miRNA). Furthermore, a xenograft mouse model was used to validate the function of circ_0049447 in vivo.

Results:

We demonstrated that circ_0049447 was downregulated in GC (P < 0.001). The area under the receiver operating characteristic curve reached 0.838, while sensitivity was 82.3% and specificity was 77.2%. CCK-8 and colony formation assay showed that overexpression of circ_0049447 could inhibit the proliferation (P < 0.05). Transwell migration and invasion assay showed upregulated circ_0049447 could impede migration in GC cells (P < 0.05). In addition, overexpression of circ_0049447 could impede GC cell EMT. Upregulation of miR-324-5p in GC specimens and direct binding between miR-324-5p with circ_0049447 proven by luciferase reporter assay indicated that circ_0049447 may inhibit GC by sponging certain miRNA.

Conclusion:

Circ_0049447 acts as a tumor suppressor in GC through reducing proliferation, migration, invasion, and EMT, and it is a promising biomarker for diagnosis.
Associations between air pollution indicators and prevalent and incident diabetes in an African American cohort, the Jackson Heart Study
Authors: Weaver Anne M. , Bidulescu Aurelian , Wellenius Gregory A. , Hickson DeMarc A. , Sims Mario , Vaidyanathan Ambarish , Wu Wen-Chih , Correa Adolfo , Wang Yi
Published: April 2021

Background:

Diabetes is especially prevalent among African Americans. Prior studies suggest that long-term exposure to ambient air pollution may be associated with greater incidence of diabetes, but results remain heterogeneous. Few studies have included large numbers of African Americans.

Methods:

We assessed diabetes status and concentrations of 1- and 3-year fine particulate matter (PM2.5) and ozone (O3) among African American participants of the Jackson Heart Study at visits 1 (2000–2004, N = 5128) and 2 (2005–2008, N = 2839). We used mixed-effect modified Poisson regression to estimate risk ratios (RRs) and 95% confidence intervals (CIs) of incidence of diabetes by visit 2 and prevalence ratios (PRs) of the association between air pollution exposure and prevalent diabetes at visits 1 and 2. We adjusted for potential confounding by patient characteristics, as well as inverse probability weights of diabetes at visit 2, accounting for clustering by census tract.

Results:

We observed associations between incident diabetes and interquartile range increase in 1-year O3 (RR 1.34, 95% CI = 1.11, 1.61) and 3-year O3 (RR 0.88, 95% CI = 0.76, 1.02). We observed associations between prevalent diabetes and 1-year PM2.5 (PR 1.08, 95% CI = 1.00, 1.17), 1-year O3 (PR 1.18, 95% CI = 1.10, 1.27), and 3-year O3 (PR 0.95, 95% CI = 0.90, 1.01) at visit 2.

Conclusions:

Our results provide some evidence of positive associations between indicators of long-term PM2.5 and O3 exposure and diabetes. This study is particularly relevant to African Americans, who have higher prevalence of diabetes but relatively few studies of environmental pollution risk factors.