bluuebunny/crossref_metadata_2025 huggingface dataset:
Linear and Nonlinear Earthquake Analysis for Strength Evaluation of Masonry Monument of Neoria
Authors: Stavroulaki Maria E. , Kasampali Amalia , Charalambidi Barbara , Motsa Siphesihle Mpho , Drosopoulos Georgios A. , Stavroulakis Georgios E.
Published: August 2023

An evaluation of the seismic behavior of a massive masonry monument with vaults, namely, the Neoria complex at the old port of Chania, is presented here. The usage of modal response analysis requires the combination of many eigenmodes in order to capture the required amount of vibration energy. Alternatively, a number of earthquakes can be used within a time domain response analysis in order to evaluate the response and, subsequently, the strength of the structure. Results of linear analysis are compared here, since this is what is required from current seismic codes. A nonlinear analysis with adequate material models will also be presented in order to demonstrate a comparison with linear analysis and a prediction of damage appearance under ultimate conditions. From the present investigation, it is shown that the results of the modal analysis and the linear time-step analysis are comparable. Therefore, some confidence is gained towards using the results for the design of strengthening and rehabilitation studies. Nonlinear models are very sensitive with respect to design earthquakes and material models. Therefore, at this stage, their results are used for the identification of areas where interventions must be performed very carefully.
Evaluation of Safety Management of Smart Construction Sites from the Perspective of Resilience
Authors: Qian Yutong , Liu Hui , Mao Peng , Zheng Xiaodan
Published: August 2023

In the context of green, low-carbon, and sustainable construction, the safety management of smart construction sites has been a key issue. Current related research mainly focuses on the application of technology, but lacks methods to evaluate the safety management level. Therefore, this research aims to construct a smart construction site safety management evaluation model from a resilience perspective. First, this research identified and screened the indicators initially based on the 4R resilience characteristics and 4M theory by analyzing the policy texts of smart construction site safety management. Then, through expert consultation, the ISM model of resilience indicators was established to determine the evaluation indicator system of smart construction site safety management. Next, the weight of each indicator was determined with the help of the analytic network process, and the evaluation criteria of the indicators were formulated according to the existing specifications and expert interviews; then, the evaluation model of smart construction site safety management was established. Finally, the feasibility of the model was proved through a case study. The findings of the research show that in terms of weights, management has the highest score, followed by media, man, and machine. However, more resilience measures are used for the safety management of machine than the other three in policy texts. Obviously, there is a deviation between weights and resilience characteristics. These findings help reveal the current situation of safety management at smart construction sites, which is of great significance for improving resilience. The findings also help smart construction sites to realize the upgrading of safety, efficiency, and greenness, and promote the sustainable development of smart construction sites as well as the construction industry.
Real-Time Early Safety Warning for Personnel Intrusion Behavior on Construction Sites Using a CNN Model
Authors: Zhao Jinyu , Xu Yinghui , Zhu Weina , Liu Mei , Zhao Jing
Published: August 2023

The high number of annual safety accidents and casualties reflects the problems of slow detection of safety accidents and untimely early warnings in current construction safety management, and China urgently needs new methods and technologies to improve the safety management efficiency of the construction industry. However, there are fewer achievements in the use of new technologies for intelligent construction safety management, and most of the research focuses on intrusion detection and specific event alarms, which cannot be well implemented for systematic early warning functions. Based on the existing research and the characteristics of early warning scenarios, this study introduces the convolutional neural network (CNN) to build a video image recognition and classification model to give early safety warnings for intrusion behavior in hazard areas of construction and demonstrates the warning effect and accuracy with practical cases. First, it clarifies the early warning demand information, such as the attributes of construction personnel and hazard areas. Then, the construction model is realized by multi-scale hierarchical feature extraction mapping, the Softmax classification function, and the argmax function. Finally, from the empirical analysis, it can be seen that an early safety warning based on the CNN model has an accurate ability to identify the intrusion behavior of construction site personnel, which can reduce the probability of construction safety accidents to a certain extent, and provide enlightenment for further realization of intelligent construction sites.
The Hidden Facets: Uncovering the Influence of Region on Social Housing Unit Distribution in Brazil
Authors: Moreira Frederico G. P. , Silva Lucas E. C. , dos Santos Victor I. M.
Published: August 2023

The Brazilian housing program, Minha Casa, Minha Vida (MCMV) (My House, My Life), was launched in 2009 to address the housing deficit issue, with the goal of distributing contracted housing units throughout the Brazilian territory. However, the program faces criticisms regarding the distribution of these units. Thus, this paper aims to analyze the distribution heterogeneity of these contracted housing units (CHUs). Two analytical approaches were employed: temporal and spatial (states). To achieve this objective, inferential methods such as Ordinary Least Squares (OLS), Spatial Autoregressive Model (SAR), and panel data regressions were employed. The findings indicate that, from a temporal perspective, there is a positive relationship between the urban housing deficit (UHD) and CHUs. However, the relationship is negative from a spatial perspective, characterizing such heterogeneity among the states. In addition, bordering regions are subject to mutual spatial influences in terms of contracted units, thereby reinforcing this heterogeneity over time.
Integrating BIM–IoT and Autonomous Mobile Robots for Construction Site Layout Printing
Authors: Iqbal Fahad , Ahmed Shiraz , Amin Fayiz , Qayyum Siddra , Ullah Fahim
Published: August 2023

The traditional methods of marking construction site layouts using manual techniques such as chalk lines are prone to human errors, resulting in discrepancies between blueprints and actual layouts. This has serious implications for project delivery, construction, costs and, eventually, project success. However, this issue can be resolved through autonomous robots and construction automation in line with Industry 4.0 and 5.0 goals. Construction automation enables workers to concentrate on the construction phase and not worry about manual site markups. This leads to an enhancement in their productivity. This study aims to improve the floor layout printing technique by introducing a framework that integrates building information modeling (BIM) and the Internet of Things (IoT), i.e., BIM–IoT and autonomous mobile robots (AMR). The development process focuses on three key components: a marking tool, an IoT-based AMR and BIM. The BIM-based tools extract and store coordinates on the cloud platform. The AMR, developed using ESP32 and connected to the Google Firestore cloud platform, leverages IoT technology to retrieve the data and draw site layout lines accordingly. Further, this research presents a prototype of an automated robot capable of accurately printing construction site layouts. A design science research (DSR) method is employed in this study that includes a comprehensive review of the existing literature and usage of AMRs in construction layout printing. Subsequently building upon the extant literature, an AMR is developed and experiments are conducted to evaluate the system’s performance. The experiment reveals that the system’s precision falls within a range of ±15 mm and its angle accuracy is within ±4 degrees. Integrating robotic automation, IoT and BIM technologies enhances the efficiency and precision of construction layout printing. The findings provide insights into the potential benefits of deploying AMRs in construction projects, reducing site layout errors and improving construction productivity. This study also adds to the body of knowledge around construction automation in line with Industry 4.0 and 5.0 endeavors.
Functional Characterization of Transforming Growth Factor-β Signaling in Dasatinib Resistance and Pre-BCR+ Acute Lymphoblastic Leukemia
Authors: Mostufi-Zadeh-Haghighi Gila , Veratti Pia , Zodel Kyra , Greve Gabriele , Waterhouse Miguel , Zeiser Robert , Cleary Michael L. , Lübbert Michael , Duque-Afonso Jesús
Published: August 2023

The multi-kinase inhibitor dasatinib has been implicated to be effective in pre-B-cell receptor (pre-BCR)-positive acute lymphoblastic leukemia (ALL) expressing the E2A-PBX1 fusion oncoprotein. The TGFβ signaling pathway is involved in a wide variety of cellular processes, including embryonic development and cell homeostasis, and it can have dual roles in cancer: suppressing tumor growth at early stages and mediating tumor progression at later stages. In this study, we identified the upregulation of the TGFβ signaling pathway in our previously generated human dasatinib-resistant pre-BCR+/E2A-PBX1+ ALL cells using global transcriptomic analysis. We confirm the upregulation of the TGFβ pathway member SMAD3 at the transcriptional and translational levels in dasatinib-resistant pre-BCR+/E2A-PBX1+ ALL cells. Hence, dasatinib blocks, at least partially, TGFβ-induced SMAD3 phosphorylation in several B-cell precursor (BCP) ALL cell lines as well as in dasatinib-resistant pre-BCR+/E2A-PBX1+ ALL cells. Activation of the TGFβ signaling pathway by TGF-β1 leads to growth inhibition by cell cycle arrest at the G0/G1 stage, increase in apoptosis and transcriptional changes of SMAD-targeted genes, e.g. c-MYC downregulation, in pre-BCR+/E2A-PBX1+ ALL cells. These results provide a better understanding about the role that the TGFβ signaling pathway plays in leukemogenesis of BCP-ALL as well as in secondary drug resistance to dasatinib.
Combined Nivolumab and Ipilimumab in Octogenarian and Nonagenarian Melanoma Patients
Authors: Reichert Constance , Baldini Capucine , Mezghani Sarah , Maubec Eve , Longvert Christine , Mortier Laurent , Quereux Gaëlle , Jannic Arnaud , Machet Laurent , de Quatrebarbes Julie , Nardin Charlée , Beneton Nathalie , Amini Adle Mona , Funck-Brentano Elisa , Descamps Vincent , Hachon Lorry , Malissen Nausicaa , Baroudjian Barouyr , Brunet-Possenti Florence
Published: August 2023

Data regarding elderly melanoma patients treated with anti-PD-1 or anti-CTLA-4 antibodies are in favor of tolerability outcomes that are similar to those of younger counterparts. However, there are very few studies focusing on elderly patients receiving nivolumab combined with ipilimumab (NIVO + IPI). Here, we ask what are the current prescribing patterns of NIVO + IPI in the very elderly population and analyze the tolerance profile. This French multicenter retrospective study was conducted on 60 melanoma patients aged 80 years and older treated with NIVO + IPI between January 2011 and June 2022. The mean age at first NIVO + IPI administration was 83.7 years (range: 79.3–93.3 years). Fifty-five patients (92%) were in good general condition and lived at home. Two dosing regimens were used: NIVO 1 mg/kg + IPI 3 mg/kg Q3W (NIVO1 + IPI3) in 27 patients (45%) and NIVO 3 mg/kg + IPI 1 mg/kg Q3W (NIVO3 + IPI1) in 33 patients (55%). NIVO + IPI was a first-line treatment in 39 patients (65%). The global prevalence of immune-related adverse events was 63% (38/60), with 27% (16/60) being of grade 3 or higher. Grade ≥ 3 adverse events were less frequent in patients treated with NIVO3 + IPI1 compared with those treated with NIVO1 + IPI3 (12% versus 44%, p = 0.04). In conclusion, the prescribing patterns of NIVO + IPI in very elderly patients are heterogeneous in terms of the dosing regimen and line of treatment. The safety profile of NIVO + IPI is reassuring; whether or not the low-dose regimen NIVO3 + IPI1 should be preferred over NIVO1 + IPI3 in patients aged 80 years or older remains an open question.
Appraisal of Systemic Treatment Strategies in Early HER2-Positive Breast Cancer—A Literature Review
Authors: Giffoni de Mello Morais Mata Danilo , Chehade Rania , Hannouf Malek B. , Raphael Jacques , Blanchette Phillip , Al-Humiqani Abdullah , Ray Monali
Published: August 2023

Background: The overexpression of the human epidermal growth factor receptor 2 (HER2+) accounts for 15–20% of all breast cancer phenotypes. Even after the completion of the standard combination of chemotherapy and trastuzumab, relapse events occur in approximately 15% of cases. The neoadjuvant approach has multiple benefits that include the potential to downgrade staging and convert previously unresectable tumors to operable tumors. In addition, achieving a pathologic complete response (pCR) following preoperative systemic treatment is prognostic of enhanced survival outcomes. Thus, optimal evaluation among the suitable strategies is crucial in deciding which patients should be selected for the neoadjuvant approach. Methods: A literature search was conducted in the Embase, Medline, and Cochrane electronic libraries. Conclusion: The evaluation of tumor and LN staging and, hence, stratifying BC recurrence risk are decisive factors in guiding clinicians to optimize treatment decisions between the neoadjuvant versus adjuvant approaches. For each individual case, it is important to consider the most likely postsurgical outcome, since, if the patient does not obtain pCR following neoadjuvant treatment, they are eligible for adjuvant T-DM1 in the case of residual disease. This review of HER2-positive female BC outlines suitable neoadjuvant and adjuvant systemic treatment strategies for guiding clinical decision making around the selection of an appropriate therapy.
Associations of Multiparametric Breast MRI Features, Tumor-Infiltrating Lymphocytes, and Immune Gene Signature Scores Following a Single Dose of Trastuzumab in HER2-Positive Early-Stage Breast Cancer
Authors: Kennedy Laura C. , Kazerouni Anum S. , Chau Bonny , Biswas Debosmita , Alvarez Rebeca , Durenberger Grace , Dintzis Suzanne M. , Stanton Sasha E. , Partridge Savannah C. , Gadi Vijayakrishna
Published: August 2023

Dynamic biomarkers that permit the real-time monitoring of the tumor microenvironment response to therapy are an unmet need in breast cancer. Breast magnetic resonance imaging (MRI) has demonstrated value as a predictor of pathologic complete response and may reflect immune cell changes in the tumor microenvironment. The purpose of this pilot study was to investigate the value of breast MRI features as early markers of treatment-induced immune response. Fourteen patients with early HER2+ breast cancer were enrolled in a window-of-opportunity study where a single dose of trastuzumab was administered and both tissue and MRIs were obtained at the pre- and post-treatment stages. Functional diffusion-weighted and dynamic contrast-enhanced MRI tumor measures were compared with tumor-infiltrating lymphocytes (TILs) and RNA immune signature scores. Both the pre-treatment apparent diffusion coefficient (ADC) and the change in peak percent enhancement (DPE) were associated with increased tumor-infiltrating lymphocytes with trastuzumab therapy (r = −0.67 and -0.69, p < 0.01 and p < 0.01, respectively). Low pre-treatment ADC and a greater decrease in PE in response to treatment were also associated with immune-activated tumor microenvironments as defined by RNA immune signatures. Breast MRI features hold promise as biomarkers of early immune response to treatment in HER2+ breast cancer.
Artificial Intelligence in Lung Cancer Screening: The Future Is Now
Authors: Cellina Michaela , Cacioppa Laura Maria , Cè Maurizio , Chiarpenello Vittoria , Costa Marco , Vincenzo Zakaria , Pais Daniele , Bausano Maria Vittoria , Rossini Nicolò , Bruno Alessandra , Floridi Chiara
Published: August 2023

Lung cancer has one of the worst morbidity and fatality rates of any malignant tumour. Most lung cancers are discovered in the middle and late stages of the disease, when treatment choices are limited, and patients’ survival rate is low. The aim of lung cancer screening is the identification of lung malignancies in the early stage of the disease, when more options for effective treatments are available, to improve the patients’ outcomes. The desire to improve the efficacy and efficiency of clinical care continues to drive multiple innovations into practice for better patient management, and in this context, artificial intelligence (AI) plays a key role. AI may have a role in each process of the lung cancer screening workflow. First, in the acquisition of low-dose computed tomography for screening programs, AI-based reconstruction allows a further dose reduction, while still maintaining an optimal image quality. AI can help the personalization of screening programs through risk stratification based on the collection and analysis of a huge amount of imaging and clinical data. A computer-aided detection (CAD) system provides automatic detection of potential lung nodules with high sensitivity, working as a concurrent or second reader and reducing the time needed for image interpretation. Once a nodule has been detected, it should be characterized as benign or malignant. Two AI-based approaches are available to perform this task: the first one is represented by automatic segmentation with a consequent assessment of the lesion size, volume, and densitometric features; the second consists of segmentation first, followed by radiomic features extraction to characterize the whole abnormalities providing the so-called “virtual biopsy”. This narrative review aims to provide an overview of all possible AI applications in lung cancer screening.
Phosphotungstic Wells-Dawson Heteropolyacid as Potential Catalyst in the Transesterification of Waste Cooking Oil
Authors: Mateos Paula S. , Ruscitti Claudia B. , Casella Mónica L. , Matkovic Silvana R. , Briand Laura E.
Published: August 2023

The esterification of oleic acid was applied in order to screen the suitability of a series of phosphotungstic-based Wells-Dawson types of compounds as potential catalysts in the heterogeneous transesterification of sunflower waste cooking oil. This test reaction indicated that the phosphotungstic Wells-Dawson heteropolyacid H6P2W18O62·xH2O, dispersed on titania oxide in a loading of 15 mg per m2 of oxide support (named 42% HPA/TiO2) and possessing exclusively Brønsted acid sites, was the most promising among the screened materials. In addition, the application of a nonlinear analysis methodology to find a surface that fits the specific activity of the oleic acid esterification with methanol at various temperatures, weights of catalyst, molar ratios of substrates, and stirring speeds, and also considering the active phase desorption out of the catalyst’s surface, allowed determining the optimum operative conditions that were applied in the transesterification of the waste cooking oil afterwards. The transesterification of the waste cooking oil at 60 °C and 1:9 WCO: methanol molar ratio in a batch reactor under stirring at 650 rpm for 3 h, catalyzed with 0.25 wt% of 42% HPA/TiO2 (20.0 g of oil and 49.6 mg of catalyst), presents 74.6% of conversion of glycerides and 74.4% yield towards fatty acid methyl esters. The catalyst was recovered and reused several times, maintaining a fairly constant catalytic performance.
Insights into the Function of Aquaporins in Gastrointestinal Fluid Absorption and Secretion in Health and Disease
Authors: Calamita Giuseppe , Delporte Christine
Published: August 2023

Aquaporins (AQPs), transmembrane proteins permeable to water, are involved in gastrointestinal secretion. The secretory products of the glands are delivered either to some organ cavities for exocrine glands or to the bloodstream for endocrine glands. The main secretory glands being part of the gastrointestinal system are salivary glands, gastric glands, duodenal Brunner’s gland, liver, bile ducts, gallbladder, intestinal goblet cells, exocrine and endocrine pancreas. Due to their expression in gastrointestinal exocrine and endocrine glands, AQPs fulfill important roles in the secretion of various fluids involved in food handling. This review summarizes the contribution of AQPs in physiological and pathophysiological stages related to gastrointestinal secretion.
Adverse Crosstalk between Extracellular Matrix Remodeling and Ferroptosis in Basal Breast Cancer
Authors: Desterke Christophe , Cosialls Emma , Xiang Yao , Elhage Rima , Duruel Clémence , Chang Yunhua , Hamaï Ahmed
Published: August 2023

(1) Background: Breast cancer is a frequent heterogeneous disorder diagnosed in women and causes a high number of mortality among this population due to rapid metastasis and disease recurrence. Ferroptosis can inhibit breast cancer cell growth, improve the sensitivity of chemotherapy and radiotherapy, and inhibit distant metastases, potentially impacting the tumor microenvironment. (2) Methods: Through data mining, the ferroptosis/extracellular matrix remodeling literature text-mining results were integrated into the breast cancer transcriptome cohort, taking into account patients with distant relapse-free survival (DRFS) under adjuvant therapy (anthracyclin + taxanes) with validation in an independent METABRIC cohort, along with the MDA-MB-231 and HCC338 transcriptome functional experiments with ferroptosis activations (GSE173905). (3) Results: Ferroptosis/extracellular matrix remodeling text-mining identified 910 associated genes. Univariate Cox analyses focused on breast cancer (GSE25066) selected 252 individual significant genes, of which 170 were found to have an adverse expression. Functional enrichment of these 170 adverse genes predicted basal breast cancer signatures. Through text-mining, some ferroptosis-significant adverse-selected genes shared citations in the domain of ECM remodeling, such as TNF, IL6, SET, CDKN2A, EGFR, HMGB1, KRAS, MET, LCN2, HIF1A, and TLR4. A molecular score based on the expression of the eleven genes was found predictive of the worst prognosis breast cancer at the univariate level: basal subtype, short DRFS, high-grade values 3 and 4, and estrogen and progesterone receptor negative and nodal stages 2 and 3. This eleven-gene signature was validated as regulated by ferroptosis inductors (erastin and RSL3) in the triple-negative breast cancer cellular model MDA-MB-231. (4) Conclusions: The crosstalk between ECM remodeling-ferroptosis functionalities allowed for defining a molecular score, which has been characterized as an independent adverse parameter in the prognosis of breast cancer patients. The gene signature of this molecular score has been validated to be regulated by erastin/RSL3 ferroptosis activators. This molecular score could be promising to evaluate the ECM-related impact of ferroptosis target therapies in breast cancer.
The Prevalence and Clinical Impact of Transition Zone Anastomosis in Hirschsprung Disease: A Systematic Review and Meta-Analysis
Authors: Labib Hosnieya , Roorda Daniëlle , van der Voorn J. Patrick , Oosterlaan Jaap , van Heurn L. W. Ernest , Derikx Joep P. M.
Published: August 2023

Background: Hirschsprung disease (HD) is characterized by absent neuronal innervation of the distal colonic bowel wall and is surgically treated by removing the affected bowel segment via pull-through surgery (PT). Incomplete removal of the affected segment is called transition zone anastomosis (TZA). The current systematic review aims to provide a comprehensive overview of the prevalence and clinical impact of TZA. Methods: Pubmed, Embase, Cinahl, and Web of Sciences were searched (last search: October 2020), and studies describing histopathological examination for TZA in patients with HD were included. Data were synthesized into aggregated Event Rates (ER) of TZA using random-effects meta-analysis. The clinical impact was defined in terms of obstructive defecation problems, enterocolitis, soiling, incontinence, and the need for additional surgical procedures. The quality of studies was assessed using the Newcastle–Ottawa Scale. Key Results: This systematic review included 34 studies, representing 2207 patients. After excluding series composed of only patients undergoing redo PT, the prevalence was 9% (ER = 0.09, 95% CI = 0.05–0.14, p < 0.001, I2 = 86%). TZA occurred more often after operation techniques other than Duhamel (X2 = 19.21, p = <0.001). Patients with TZA often had obstructive defecation problems (62%), enterocolitis (38%), soiling (28%), and fecal incontinence (24%) in follow-up periods ranging from 6 months to 13 years. Patients with TZA more often had persistent obstructive symptoms (X2 = 7.26, p = 0.007). Conclusions and Inferences: TZA is associated with obstructive defecation problems and redo PT and is thus necessary to prevent.
Visual Analysis of Panoramic Radiographs among Pediatric Dental Residents Using Eye-Tracking Technology: A Cross-Sectional Study
Authors: Bhadila Ghalia Y. , Alsharif Safiya I. , Almarei Seba , Almashaikhi Jamila A. , Bahdila Dania
Published: August 2023

The aim of this cross-sectional study was to explore the eye tracking (ET) performance of postgraduate pediatric dental students in correctly detecting abnormalities in different sets of panoramic radiographs. This observational study recruited postgraduate pediatric dental students to evaluate seven panoramic radiographs. RED-m® SMI software (Sensomotoric Instruments, Teltow, Germany) was used to track the participants’ eye movements as they looked at the radiographs. The data collected for areas of interest (AOIs) included revisit counts, fixation counts, fixation times, entry times, and dwell times. Univariate and bivariate analyses were conducted to summarize the participants’ characteristics and ET measures. The overall percentage of correctly located AOIs was 71.7%. The residents had significantly more revisits and fixation counts in AOIs located in one sextant than in multiple sextants (p < 0.001). Similar patterns were observed for fixation and dwell times (p < 0.001), but not for entry time. Heatmaps showed that the highest density of fixations was on the AOIs and the residents fixated more on dentition than on bony structures. In single-sextant radiographs, residents had significantly more revisits and fixation counts for AOIs compared to those of multiple sextants. Residents had slower entry times and dwelled less on AOIs located in multiple sextant(s). The reported findings can direct dental educators to develop a standardized scan scheme of panoramic radiographs to minimize misdiagnosis.
The Effect of House Dust Sensitization on Skin Sebum and Moisture in Children with Allergic Respiratory Diseases
Authors: Altaş Uğur , Altaş Zeynep Meva , Ercan Nazlı , Özkars Mehmet Yaşar
Published: August 2023

This study aimed to investigate the levels of skin moisture and sebum in children with a house dust allergy without skin symptoms. This was a case–control study involving children, aged 0–18 years, who were being followed up for an allergic airway disease in a pediatric allergy clinic. Age, gender, hemogram parameters, and IgE values were evaluated. The skin moisture and sebum percentages of the patients and control group were measured by a non-invasive bioimpedance method using a portable digital skin moisture and sebum measurement device on the cubital fossa. The median value of the skin moisture percentage in the house dust mite allergy-positive patient group was significantly lower than that in the house dust mite allergy-negative patients and the control group (p < 0.001). The house dust mite allergy-positive patient group had the lowest skin sebum content. However, there was no statistical significance among the groups in terms of skin sebum percentage (p = 0.102). In the study, children with a house dust allergy were found to have lower levels of skin moisture and sebum. The regular use of moisturizers for children with a house dust allergy should be kept in mind as an effective solution to protect the skin barrier and reduce skin symptoms.
Six Express Sequence Tag–Simple Sequence Repeat Primers Reveal Genetic Diversity in the Cultivars of Three Zanthoxylum Species
Authors: Deng Yangchuan , He Zhoujian , Li Yanlin , Ye Meng , Xiang Li
Published: August 2023

Zanthoxylum (Sichuan pepper), with its rich cultivars, has long been widely cultivated in China for its unique seasoning and medicinal uses, but most of its cultivars have similar morphological characteristics. Therefore, we hypothesized that the genetic diversity of Zanthoxylum cultivars is low because of their apomixis and long cultivation history. In this study, we aimed to investigate the genetic diversity of three Zanthoxylum species on the cultivar level based on express sequence tag–simple sequence repeat (EST-SSR) primers. In total, 121 samples of three Zanthoxylum species (Z. bungeanum, Z. armatum and Z. piperitum) were collected from different areas in China for genetic diversity analysis. A total of six specificity and polymorphism EST-SSR primers, which we selected from among 120 primers based on two transcriptomes (Z. bungeanum, Z. armatum) in our earlier study, were used to evaluate genetic diversity based on capillary electrophoresis technology. The results of our analysis using the unweighted pair group method with arithmetic mean (UPGMA) indicated that most of the samples are clustered in one clade in the UPGMA dendrogram, and the average genetic distance was 0.6409. Principal component analysis (PCA) showed that Z. piperitum may have a closer genetic relationship with Z. bungeanum than with Z. armatum. An analysis of molecular variation (AMOVA) showed that the genetic variation mainly stemmed from individuals within populations; the genetic differentiation coefficient (PhiPT) was 0.429, the gene flow (Nm) between populations was 0.333, and the differences among populations were not significant (p > 0.001). For the intraspecific populations of ZB, the percentage of genetic variation was 53% among populations and 47% within populations, with non-significant differences between populations (p > 0.001). The genetic differentiation coefficient (PhiT) was 0.529, and the gene flow (Nm) was 0.223. For the intraspecific populations of ZA, the results indicated that the percentage of genetic variation was 29% among populations and 71% within populations, with non-significant differences between populations (p > 0.001); the genetic differentiation coefficient (PhiPT) was 0.293, and the gene flow (Nm) was 0.223. Through genetic structure analysis (GSA), we predicted that these 121 samples belonged to two optimal subgroups, which means that all the samples probably originated from two gene pools. Above all, this indicated that the genetic diversity of the 121 Zanthoxylum samples was relatively low at both the species and cultivar levels, a finding which was consistent with our initial assumptions. This study provides a reference, with molecular-level data, for the further identification of Zanthoxylum species.
Keep It Simple: Using README Files to Advance Standardization in Chronobiology
Authors: Zieliński Tomasz , Hodge James J. L. , Millar Andrew J.
Published: August 2023

Standardization plays a crucial role in ensuring the reliability, reproducibility, and interoperability of research data in the biomedical sciences. Metadata standards are one foundation for the FAIR (Findable, Accessible, Interoperable, and Reusable) principles of data management. They facilitate data discovery, understanding, and reuse. However, the adoption of metadata standards in biological research lags in practice. Barriers such as complexity, lack of incentives, technical challenges, resource constraints, and resistance to change hinder widespread adoption. In the field of chronobiology, standardization is essential but faces particular challenges due to the longitudinal nature of experimental data, diverse model organisms, and varied measurement techniques. To address these challenges, we propose an approach that emphasizes simplicity and practicality: the development of README templates tailored for particular data types and species. Through this opinion article, our intention is to initiate a dialogue and commence a community-driven standardization process by engaging potential contributors and collaborators.
Thickness and Humidity on Proton Conductivity in MOF-508 Thin Film by Twin-Zinc-Source Method
Authors: Zhang Kun , Wang Chunxia , Yang Feng , Li Jing , Yan Shuguang , Qi Yue
Published: August 2023

To achieve structurally stable and high proton conductive materials, preferably under ambient humidity and pressure, the well-controlled thickness and conductivity of the MOF thin films represent an effective approach. Electrodes are the most important part of fuel cells; proton conducting materials are often used for electrodes, but today high proton conducting materials are expensive and use harsh conditions. Therefore, the goal of researchers is the pursuit of stable structure high proton conductive materials. We prepared well controlled thickness and conductive MOF-508a thin films on a Zn substrate by the “twin zinc source” method, which is very rare in conventional proton conductive materials. The results show that when the thickness of the MOF-508a/Zn thin film was at its minimum (16 µm), the resistivity and proton conductivity reached 2.5 × 103 Ω cm and 4 × 10−4 S cm−1, respectively. The MOF-508b/Zn thin film can absorb water molecules in a high humidity atmosphere and the conductivity decreases significantly with increasing humidity. When the film was put into the atmosphere with a relative humidity of 85%, the resistivity reached 200 Ω cm significantly. This work provides a simple, low cost, and environmentally friendly strategy for fabricating high proton conducting MOF films by exploring the “twin-zinc-source” method, which is critically important for PEMFC. It is believed that higher conductivity MOF films can be obtained with further modifications, indicating the potential of such films as humidity detectors.
Solubility and Crystallization of Glucosamine Hydrochloride in Water with the Presence of Additives
Authors: Pan Zhiying , Wang Yan , Xie Yang , Tan Jie , Zhang Qian , Lu Jianxing , Du Shichao , Xue Fumin
Published: August 2023

Glucosamine hydrochloride (GAH) is a kind of natural hexose, which is used to promote the synthesis of mucopolysaccharides and improve the metabolism of articular cartilage. In this paper, the solubility of GAH in pure water and aqueous system with the presence of three kinds of additives (HCl, NaCl, KCl) at temperatures ranging from 278.15 K to 323.15 K was determined by gravimetric method. When there are additives in water, the solubility of GAH increases with the increase of temperature and decreases with the increase of concentration of the three kinds of additives. When the additives were at similar mole fractions, HCl led to the lowest solubility of GAH. The modified Apelblat model and van’t Hoff model were used to correlate the solubility data. The average relative deviation (ARD) data of Apelblat and van’t Hoff models were less than 5%, indicating good fitting results. Based on the thermodynamic data, the cooling crystallization process of GAH was performed. It was found that the additives could affect the crystal morphology, particle size, and yield of GAH products. This study supplemented the thermodynamic data of GAH and studied the cooling crystallization process in the presence of GAH additives, which provided important guidance for the optimization of the crystallization process.
Effect of Pore Defects on Very High Cycle Fatigue Behavior of TC21 Titanium Alloy Additively Manufactured by Electron Beam Melting
Authors: Li Qingdong , Liu Shuai , Liao Binbin , Nie Baohua , Shi Binqing , Qi Haiying , Chen Dongchu , Liu Fangjun
Published: August 2023

Titanium alloys additively manufactured by electron beam melting (EBM) inevitably obtained some pore defects, which significantly reduced the very high cycle fatigue performance. An ultrasonic fatigue test was carried out on an EBM TC21 titanium alloy with hot isostatic pressing (HIP) and non-HIP treatment, and the effect of pore defects on the very high cycle fatigue (VHCF) behavior were investigated for the EBM TC21 titanium alloy. The results showed that the S-N curve of non-HIP specimens clearly had a tendency to decrease in very high cycle regimes, and HIP treatment significantly improved fatigue properties. Fatigue limits increased from 250 MPa for non-HIP specimens to 430 MPa for HIP ones. Very high cycle fatigue crack mainly initiated from the internal pore for EBM specimens, and a fine granular area (FGA) was observed at the crack initiation site in a very high cycle regime for both non-HIP and HIP specimens. ΔKFGA had a constant trend in the range from 2.7 MPam to 3.5 MPam, corresponding to the threshold stress intensity factor range for stable crack propagation. The effect of pore defects on the very high cycle fatigue limit was investigated based on the Murakami model. Furthermore, a fatigue indicator parameter (FIP) model based on pore defects was established to predict fatigue life for non-HIP and HIP specimens, which agreed with the experimental data.
Changes in Stress Reduction Following a 28-Day Prostate Cancer Patient Empowerment Program (PC-PEP) among Prostate Cancer Survivors
Authors: Burge Laura , Ilie Gabriela , MacDonald Cody , Riel Hayley , Rutledge Rob David Harold
Published: August 2023

Prostate cancer (PCa) survivors often experience post-treatment challenges that impact their well-being and mental health. The Prostate Cancer Patient Empowerment Program (PC-PEP) aims to address these issues through a comprehensive intervention, involving daily meditation/breathing exercises, physical activity, pelvic floor exercises, emotional connection strategies, and peer support. This study presents a secondary analysis of a Phase 2 feasibility study that evaluated the effects of a 28-day PC-PEP intervention on stress reduction. Thirty patients with PCa from the Maritimes, Canada, underwent pre- and post-intervention assessments to measure brainwave activity (delta, theta, alpha, beta, and gamma) using the Muse™ headband, and heart rate variability (HRV) using the HeartMath® Inner Balance™ as indicators of stress reduction. A statistically significant Time × Sensor Scalp Assessment Time interaction emerged for all brain waves. Amplitudes were generally higher during the first half of the meditation assessment time but became comparable afterward. A statistically significant Time × Sensor Scalp Location × Sensor Scalp Assessment Time interaction also emerged for alpha waves, indicating higher prefrontal lobe amplitudes than temporal lobe amplitudes from pre- to post-assessment. There were no statistically significant differences in HRV metrics from pre- to post-intervention, except for a marginally significant achievement score, indicating increased HRV coherence post-intervention. The findings suggest that the stress reduction component of PC-PEP successfully improved outcomes related to decreased stress. These results have implications for the development of future iterations of PC-PEP interventions, aiming to optimize participant benefits.
Report from the 24th Annual Western Canadian Gastrointestinal Cancer Consensus Conference on Colorectal Cancer, Richmond, British Columbia, 28–29, October 2022
Authors: Gill Sharlene , Ahmed Shahid , Anderson Brady , Berry Scott , Lim Howard , Phang Terry , Sharma Ankur , Solar Vasconcelos Joao , Gill Karamjit , Iqbal Mussawar , Tankel Keith , Chan Theresa , Recsky Magdalena , Nuk Jennifer , Paul James , Mahmood Shazia , Mulder Karen
Published: August 2023

The 24th annual Western Canadian Gastrointestinal Cancer Consensus Conference (WCGCCC) was held in Richmond, British Columbia, on 28–29 October 2022. The WCGCCC is an interactive multidisciplinary conference attended by healthcare professionals from across Western Canada (British Columbia, Alberta, Saskatchewan, and Manitoba) who are involved in the care of patients with gastrointestinal cancer. Surgical, medical, and radiation oncologists; pathologists; radiologists; and allied health care professionals such as dieticians, nurses and a genetic counsellor participated in presentation and discussion sessions for the purpose of developing the recommendations presented here. This consensus statement addresses current issues in the management of colorectal cancer.
Requiem for Rimonabant: Therapeutic Potential for Cannabinoid CB1 Receptor Antagonists after the Fall
Authors: Bosquez-Berger Taryn , Szanda Gergő , Straiker Alex
Published: August 2023

The endocannabinoid system is found throughout the CNS and the body where it impacts many important physiological processes. Expectations were high that targeting cannabinoid receptors would prove therapeutically beneficial; pharmaceutical companies quickly seized on the appetitive and metabolic effects of cannabinoids to develop a drug for the treatment of weight loss. Alas, the experience with first-in-class cannabinoid type-1 receptor (CB1R) antagonist rimonabant is a now-classic cautionary tale of the perils of drug development and the outcome of rimonabant’s fall from grace dealt a blow to those pursuing therapies involving CB1R antagonists. And this most commercially compelling application of rimonabant has now been partially eclipsed by drugs with different mechanisms of action and greater effect. Still, blocking CB1 receptors causes intriguing metabolic effects, some of which appear to occur outside the CNS. Moreover, recent years have seen a startling change in the legal status of cannabis, accompanied by a popular embrace of ‘all things cannabis’. These changes combined with new pharmacological strategies and diligent medicinal chemistry may yet see the field to some measure of fulfillment of its early promise. Here, we review the story of rimonabant and some of the therapeutic niches and strategies that still hold promise after the fall.
Quadruple-Rule-Out Computed Tomography Angiography (QRO-CT): A Novel Dual-Energy Computed Tomography Technique for the Diagnostic Work-Up of Acute Chest Pain
Authors: Cetin Turkhun , Kantarci Mecit , Irgul Baris , Aydin Sonay , Aydin Fahri , Koseturk Taner , Levent Akin
Published: August 2023

Background: Computerized tomography (CT) has been increasingly utilized in the differential diagnosis of acute chest pain. Combining the triple rule out CT angiography (TRO-CT) approach with dual-energy CT (DECT) can enhance the diagnostic capability by identifying myocardial perfusion deficiencies. This combination can yield a quadruple-rule-out computed tomography angiography (QRO-CT) technique. The aim of this study is to determine the efficacy of the QRO-CT. Methods: Intraluminal diseases and abnormalities in the main coronary arteries and branches were investigated. The myocardial dark spots on the color-coded iodine map were identified as perfusion deficiencies. Pulmonary arteries and aorta were also evaluated. Results: The study population consisted of 211 patients. The sensitivity, specificity, and positive and negative predictive values of QRO-CT for pulmonary embolism were 93.5%, 100%, 100%, and 95.3%, respectively. For obstructive coronary artery disease, the values were 96.1%, 93.4%, 89.2%, and 97.7%, respectively. For myocarditis, the values were 69.2%, 100%, 100%, and 93.6%, respectively. Conclusions: the QRO-CT method may successfully evaluate myocardial perfusion deficits, hence expanding the differential diagnosis capabilities of the standard TRO-CT method for myocarditis. It can provide useful information on myocardial perfusion, which may influence the choice to perform invasive catheterization in cases of coronary artery obstruction.
Evaluation of a 3-Item Health Index in Predicting Mortality Risk: A 12-Year Follow-Up Study
Authors: Knight Silvin P. , Ward Mark , Duggan Eoin , Xue Feng , Kenny Rose Anne , Romero-Ortuno Roman
Published: August 2023

This study was carried out using a large cohort (N = 4265; 416 deceased) of older, community-dwelling adults from The Irish Longitudinal Study on Ageing (TILDA). The study compared the performance of a new 3-item health index (HI) with two existing measures, the 32-item frailty index (FI) and the frailty phenotype (FP), in predicting mortality risk. The HI was based on the objective measurement of resting-state systolic blood pressure sample entropy, sustained attention reaction time performance, and usual gait speed. Mortality data from a 12-year follow up period were analyzed using Cox proportional regression. All data processing was performed using MATLAB and statistical analysis using STATA 15.1. The HI showed good discriminatory power (AUC = 0.68) for all-cause mortality, similar to FI (AUC = 0.68) and superior to FP (AUC = 0.60). The HI classified participants into Low-Risk (84%), Medium-Risk (15%), and High-Risk (1%) groups, with the High-Risk group showing a significant hazard ratio (HR) of 5.91 in the unadjusted model and 2.06 in the fully adjusted model. The HI also exhibited superior predictive performance for cardiovascular and respiratory deaths (AUC = 0.74), compared with FI (AUC = 0.70) and FP (AUC = 0.64). The HI High-Risk group had the highest HR (15.10 in the unadjusted and 5.61 in the fully adjusted models) for cardiovascular and respiratory mortality. The HI remained a significant predictor of mortality even after comprehensively adjusting for confounding variables. These findings demonstrate the effectiveness of the 3-item HI in predicting 12-year mortality risk across different causes of death. The HI performed similarly to FI and FP for all-cause mortality but outperformed them in predicting cardiovascular and respiratory deaths. Its ability to classify individuals into risk groups offers a practical approach for clinicians and researchers. Additionally, the development of a user-friendly MATLAB App facilitates its implementation in clinical settings. Subject to external validation in clinical research settings, the HI can be more useful than existing frailty measures in the prediction of cardio-respiratory risk.