bluuebunny/crossref_metadata_2025 huggingface dataset:
Aided Diagnosis Model Based on Deep Learning for Glioblastoma, Solitary Brain Metastases, and Primary Central Nervous System Lymphoma with Multi-Modal MRI
Authors: Liu Xiao , Liu Jie
Published: February 2024

(1) Background: Diagnosis of glioblastoma (GBM), solitary brain metastases (SBM), and primary central nervous system lymphoma (PCNSL) plays a decisive role in the development of personalized treatment plans. Constructing a deep learning classification network to diagnose GBM, SBM, and PCNSL with multi-modal MRI is important and necessary. (2) Subjects: GBM, SBM, and PCNSL were confirmed by histopathology with the multi-modal MRI examination (study from 1225 subjects, average age 53 years, 671 males), 3.0 T T2 fluid-attenuated inversion recovery (T2-Flair), and Contrast-enhanced T1-weighted imaging (CE-T1WI). (3) Methods: This paper introduces MFFC-Net, a classification model based on the fusion of multi-modal MRIs, for the classification of GBM, SBM, and PCNSL. The network architecture consists of parallel encoders using DenseBlocks to extract features from different modalities of MRI images. Subsequently, an L1−norm feature fusion module is applied to enhance the interrelationships among tumor tissues. Then, a spatial-channel self-attention weighting operation is performed after the feature fusion. Finally, the classification results are obtained using the full convolutional layer (FC) and Soft-max. (4) Results: The ACC of MFFC-Net based on feature fusion was 0.920, better than the radiomics model (ACC of 0.829). There was no significant difference in the ACC compared to the expert radiologist (0.920 vs. 0.924, p = 0.774). (5) Conclusions: Our MFFC-Net model could distinguish GBM, SBM, and PCNSL preoperatively based on multi-modal MRI, with a higher performance than the radiomics model and was comparable to radiologists.
Cellulolytic Aerobic Bacteria Isolated from Agricultural and Forest Soils: An Overview
Authors: Bautista-Cruz Angélica , Aquino-Bolaños Teodulfo , Hernández-Canseco Jessie , Quiñones-Aguilar Evangelina Esmeralda
Published: February 2024

This review provides insights into cellulolytic bacteria present in global forest and agricultural soils over a period of 11 years. It delves into the study of soil-dwelling cellulolytic bacteria and the enzymes they produce, cellulases, which are crucial in both soil formation and the carbon cycle. Forests and agricultural activities are significant contributors to the production of lignocellulosic biomass. Forest ecosystems, which are key carbon sinks, contain 20–30% cellulose in their leaf litter. Concurrently, the agricultural sector generates approximately 998 million tons of lignocellulosic waste annually. Predominant genera include Bacillus, Pseudomonas, Stenotrophomonas, and Streptomyces in forests and Bacillus, Streptomyces, Pseudomonas, and Arthrobacter in agricultural soils. Selection of cellulolytic bacteria is based on their hydrolysis ability, using artificial cellulose media and dyes like Congo red or iodine for detection. Some studies also measure cellulolytic activity in vitro. Notably, bacterial cellulose hydrolysis capability may not align with their cellulolytic enzyme production. Enzymes such as GH1, GH3, GH5, GH6, GH8, GH9, GH10, GH12, GH26, GH44, GH45, GH48, GH51, GH74, GH124, and GH148 are crucial, particularly GH48 for crystalline cellulose degradation. Conversely, bacteria with GH5 and GH9 often fail to degrade crystalline cellulose. Accurate identification of cellulolytic bacteria necessitates comprehensive genomic analysis, supplemented by additional proteomic and transcriptomic techniques. Cellulases, known for degrading cellulose, are also significant in healthcare, food, textiles, bio-washing, bleaching, paper production, ink removal, and biotechnology, emphasizing the importance of discovering novel cellulolytic strains in soil.
Exploring beyond Common Cell Death Pathways in Oral Cancer: A Systematic Review
Authors: Siquara da Rocha Leonardo de Oliveira , de Morais Everton Freitas , de Oliveira Lilianny Querino Rocha , Barbosa Andressa Vollono , Lambert Daniel W. , Gurgel Rocha Clarissa A. , Coletta Ricardo D.
Published: February 2024

Oral squamous cell carcinoma (OSCC) is the most common and lethal type of head and neck cancer in the world. Variable response and acquisition of resistance to traditional therapies show that it is essential to develop novel strategies that can provide better outcomes for the patient. Understanding of cellular and molecular mechanisms of cell death control has increased rapidly in recent years. Activation of cell death pathways, such as the emerging forms of non-apoptotic programmed cell death, including ferroptosis, pyroptosis, necroptosis, NETosis, parthanatos, mitoptosis and paraptosis, may represent clinically relevant novel therapeutic opportunities. This systematic review summarizes the recently described forms of cell death in OSCC, highlighting their potential for informing diagnosis, prognosis and treatment. Original studies that explored any of the selected cell deaths in OSCC were included. Electronic search, study selection, data collection and risk of bias assessment tools were realized. The literature search was carried out in four databases, and the extracted data from 79 articles were categorized and grouped by type of cell death. Ferroptosis, pyroptosis, and necroptosis represented the main forms of cell death in the selected studies, with links to cancer immunity and inflammatory responses, progression and prognosis of OSCC. Harnessing the potential of these pathways may be useful in patient-specific prognosis and individualized therapy. We provide perspectives on how these different cell death types can be integrated to develop decision tools for diagnosis, prognosis, and treatment of OSCC.
In-Depth Genome Characterization and Pan-Genome Analysis of Strain KMM 296, a Producer of Highly Active Alkaline Phosphatase; Proposal for the Reclassification of Cobetia litoralis and Cobetia pacifica as the Later Heterotypic Synonyms of Cobetia amphilecti and Cobetia marina, and Emended Description of the Species Cobetia amphilecti and Cobetia marina
Authors: Nedashkovskaya Olga , Balabanova Larissa , Otstavnykh Nadezhda , Zhukova Natalia , Detkova Ekaterina , Seitkalieva Aleksandra , Bystritskaya Evgenia , Noskova Yulia , Tekutyeva Liudmila , Isaeva Marina
Published: February 2024

A strictly aerobic, Gram-stain-negative, rod-shaped, and motile bacterium, designated strain KMM 296, isolated from the coelomic fluid of the mussel Crenomytilus grayanus, was investigated in detail due to its ability to produce a highly active alkaline phosphatase CmAP of the structural family PhoA. A previous taxonomic study allocated the strain to the species Cobetia marina, a member of the family Halomonadaceae of the class Gammaproteobacteria. However, 16S rRNA gene sequencing showed KMM 296’s relatedness to Cobetia amphilecti NRIC 0815T. The isolate grew with 0.5–19% NaCl at 4–42 °C and hydrolyzed Tweens 20 and 40 and L-tyrosine. The DNA G+C content was 62.5 mol%. The prevalent fatty acids were C18:1 ω7c, C12:0 3-OH, C18:1 ω7c, C12:0, and C17:0 cyclo. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, and also an unidentified aminolipid, phospholipid, and a few unidentified lipids. The major respiratory quinone was Q-8. According to phylogenomic and chemotaxonomic evidence, and the nearest neighbors, the strain KMM 296 represents a member of the species C. amphilecti. The genome-based analysis of C. amphilecti NRIC 0815T and C. litoralis NRIC 0814T showed their belonging to a single species. In addition, the high similarity between the C. pacifica NRIC 0813T and C. marina LMG 2217T genomes suggests their affiliation to one species. Based on the rules of priority, C. litoralis should be reclassified as a later heterotypic synonym of C. amphilecti, and C. pacifica is a later heterotypic synonym of C. marina. The emended descriptions of the species C. amphilecti and C. marina are also proposed.
Hyperalgesia, Increased Temporal Summation and Impaired Inhibitory Mechanisms in Episodic and Chronic Cluster Headache: An Observational Study
Authors: Bertotti Gabriele , Elizagaray-García Juan Ignacio , Rodríguez-Vico Jaime , Gil-Martínez Alfonso
Published: February 2024

Cluster Headache (CH) is a primary headache that causes severe pain. Some evidence suggests that central mechanisms might be involved. The objective of this study was (1) to compare hyperalgesia signs, temporal summation and conditioned pain modulation among episodic (ECH) and chronic CH (CCH) patients and controls, (2) to compare these factors between sides in the patient groups and (3) to compare the psychophysical variables between the groups. This cross-sectional study included 71 subjects divided into three groups (ECH, CCH and controls). Pressure pain thresholds, temporal summation, conditioned pain modulation and other psychosocial variables were measured. The ANOVA showed differences for all physical outcome measures (p < 0.05). Bonferroni post hoc analyses showed differences when comparing the patient groups with the healthy subjects (p < 0.05), with large effect sizes (d > 0.8). No differences between the patient groups were found for almost all the variables (p > 0.05). Significant differences for all the variables were detected when comparing the symptomatic and non-symptomatic sides in both the ECH and CCH groups (p < 0.05). The ECH and CCH groups showed mechanical hyperalgesia, increased temporal summation and impaired inhibitory mechanisms compared to the controls. Side-to-side differences were also detected within the patient groups. Patients with CCH had poorer sleep quality and quality of life than the controls.
SRPK1 Promotes Glioma Proliferation, Migration, and Invasion through Activation of Wnt/β-Catenin and JAK-2/STAT-3 Signaling Pathways
Authors: Shi Mengna , Sun Dan , Deng Lu , Liu Jing , Zhang Min-Jie
Published: February 2024

Currently, the treatment of gliomas still relies primarily on surgery and radiochemotherapy. Although there are various drugs available, including temozolomide, the overall therapeutic effect is unsatisfactory, and the prognosis remains poor. Therefore, the in-depth study of the mechanism of glioma development and a search for new therapeutic targets are the keys to improving the therapeutic treatment of gliomas and improving the prognosis of patients. Immunohistochemistry is used to detect the expression of relevant molecules in tissues, qPCR and Western blot are used to detect the mRNA and protein expression of relevant molecules, CCK-8 (Cell Counting Kit-8) is used to assess cell viability and proliferation capacity, Transwell is used to evaluate cell migration and invasion ability, and RNA transcriptome sequencing is used to identify the most influential pathways. SRPK1 (SRSF protein kinase 1) is highly expressed in gliomas but is not expressed in normal tissues. Its expression is positively correlated with the grades of gliomas and negatively correlated with prognosis. SRPK1 significantly promotes the occurrence and development of gliomas. Knocking down SRPK1 leads to a significant decrease in the proliferation, migration, and invasion abilities of gliomas. Loss of SRPK1 expression induces G2/M phase arrest and mitotic catastrophe, leading to apoptosis in cells. Overexpression of SRPK1 activates the Wnt/β-catenin (wingless-int1/β-catenin) and JAK-2/STAT-3 (Janus kinase 2/signal transducer and activator of transcription 3) signaling pathways, promoting the proliferation, migration, and invasion of gliomas. Overexpression of SRPK1 rescues the reduced cell proliferation, migration, and invasion abilities caused by the silencing of β-catenin or JAK-2. A stable shRNA-LN229 cell line was constructed, and using a nude mouse model, it was found that stable knockout of SRPK1 significantly reduced the tumorigenic ability of glioma cells, as evidenced by a significant decrease in the subcutaneous tumor volume and weight in nude mice. We have demonstrated that SRPK1 is highly expressed in gliomas. Overexpression of SRPK1 activates the Wnt/β-catenin and JAK-2/STAT-3 signaling pathways, promoting the proliferation, migration, and invasion of gliomas. Silencing SRPK1-related signaling pathways may provide potential therapeutic options for glioma patients.
The Influence of Hydroxyapatite Crystals on the Viscoelastic Behavior of Poly(vinyl alcohol) Braid Systems
Authors: Quinaz Tiago , Freire Tânia F. , Olmos Andrea , Martins Marcos , Ferreira Fernando B. N. , de Moura Marcelo F. S. M. , Zille Andrea , Nguyễn Quyền , Xavier José , Dourado Nuno
Published: February 2024

Composites of poly(vinyl alcohol) (PVA) in the shape of braids, in combination with crystals of hydroxyapatite (HAp), were analyzed to perceive the influence of this bioceramic on both the quasi-static and viscoelastic behavior under tensile loading. Analyses involving energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscopy (SEM) allowed us to conclude that the production of a homogeneous layer of HAp on the braiding surface and the calcium/phosphate atomic ratio were comparable to those of natural bone. The maximum degradation temperature established by thermogravimetric analysis (TGA) showed a modest decrease with the addition of HAp. By adding HAp to PVA braids, an increase in the glass transition temperature (Tg) is noticed, as demonstrated by dynamic mechanical analysis (DMA) and differential thermal analysis (DTA). The PVA/HAp composite braids’ peaks were validated by Fourier transform infrared (FTIR) spectroscopy to be in good agreement with common PVA and HAp patterns. PVA/HAp braids, a solution often used in the textile industry, showed superior overall mechanical characteristics in monotonic tensile tests. Creep and relaxation testing showed that adding HAp to the eight and six-braided yarn architectures was beneficial. By exhibiting good mechanical performance and most likely increased biological qualities that accompany conventional care for bone applications in the fracture healing field, particularly multifragmentary ones, these arrangements can be applied as a fibrous fixation system.
The 3D-McMap Guidelines: Three-Dimensional Multicomposite Microsphere Adaptive Printing
Authors: Klar Roland M. , Cox James , Raja Naren , Lohfeld Stefan
Published: February 2024

Microspheres, synthesized from diverse natural or synthetic polymers, are readily utilized in biomedical tissue engineering to improve the healing of various tissues. Their ability to encapsulate growth factors, therapeutics, and natural biomolecules, which can aid tissue regeneration, makes microspheres invaluable for future clinical therapies. While microsphere-supplemented scaffolds have been investigated, a pure microsphere scaffold with an optimized architecture has been challenging to create via 3D printing methods due to issues that prevent consistent deposition of microsphere-based materials and their ability to maintain the shape of the 3D-printed structure. Utilizing the extrusion printing process, we established a methodology that not only allows the creation of large microsphere scaffolds but also multicomposite matrices into which cells, growth factors, and therapeutics encapsulated in microspheres can be directly deposited during the printing process. Our 3D-McMap method provides some critical guidelines for issues with scaffold shape fidelity during and after printing. Carefully timed breaks, minuscule drying steps, and adjustments to extrusion parameters generated an evenly layered large microsphere scaffold that retained its internal architecture. Such scaffolds are superior to other microsphere-containing scaffolds, as they can release biomolecules in a highly controlled spatiotemporal manner. This capability permits us to study cell responses to the delivered signals to develop scaffolds that precisely modulate new tissue formation.
Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering
Authors: Tolmacheva Nelli , Bhattacharyya Amitava , Noh Insup
Published: February 2024

Three-dimensional bioprinting is a promising technology for bone tissue engineering. However, most hydrogel bioinks lack the mechanical and post-printing fidelity properties suitable for such hard tissue regeneration. To overcome these weak properties, calcium phosphates can be employed in a bioink to compensate for the lack of certain characteristics. Further, the extracellular matrix of natural bone contains this mineral, resulting in its structural robustness. Thus, calcium phosphates are necessary components of bioink for bone tissue engineering. This review paper examines different recently explored calcium phosphates, as a component of potential bioinks, for the biological, mechanical and structural properties required of 3D bioprinted scaffolds, exploring their distinctive properties that render them favorable biomaterials for bone tissue engineering. The discussion encompasses recent applications and adaptations of 3D-printed scaffolds built with calcium phosphates, delving into the scientific reasons behind the prevalence of certain types of calcium phosphates over others. Additionally, this paper elucidates their interactions with polymer hydrogels for 3D bioprinting applications. Overall, the current status of calcium phosphate/hydrogel bioinks for 3D bioprinting in bone tissue engineering has been investigated.
Microfluidics and Organ-on-a-Chip for Disease Modeling and Drug Screening
Authors: Nasiri Rohollah , Zhu Yangzhi , de Barros Natan Roberto
Published: February 2024

The convergence of microfluidics and organ-on-a-chip (OoC) technologies has revolutionized our ability to create advanced in vitro models that recapitulate complex physiological processes [...]
Advances and New Perspectives in Micro-Nanofabricated Sensors for Bioanalysis
Authors: Hatamie Amir
Published: February 2024

“Micro-Nanofabricated Sensors for Bioanalysis” represents a cutting-edge field in biosensing technology which leverages the integration of micro- and nanoscale fabrication techniques [...]
A Three-Dimensional Hydrophobic Surface-Enhanced Raman Scattering Sensor via a Silver-Coated Polytetrafluoroethylene Membrane for the Direct Trace Detection of Molecules in Water
Authors: Tao Guanwei , Li Jiajun , Mu Yunyun , Zhang Xinping
Published: February 2024

We report a three-dimensional (3D) SERS substrate consisting of a silver nanoparticle (AgNP) coating on the skeleton-fiber surfaces of a polytetrafluoroethylene (PTFE) membrane. Simple thermal evaporation was employed to deposit Ag onto the PTFE membrane to produce grape-shaped AgNPs. The 3D-distributed AgNPs exhibit not only strong localized surface plasmon resonance (LSPR) but also strong hydrophobic performance. High-density hotspots via silver nano-grape structures and nanogaps, the large 3D interaction volume, and the large total surface area, in combination with the hydrophobic enrichment of the specimen, facilitate high-sensitivity sensing performance of such a SERS substrate for the direct detection of low-concentration molecules in water. An enhancement factor of up to 1.97 × 1010 was achieved in the direct detection of R6G molecules in water with a concentration of 10−13 mol/L. The lowest detection limit of 100 ppt was reached in the detection of melamine in water. Such a SERS sensor may have potential applications in food-safety control, environmental water pollution monitoring, and biomedical analysis.
Advances in Respiratory Monitoring: A Comprehensive Review of Wearable and Remote Technologies
Authors: Vitazkova Diana , Foltan Erik , Kosnacova Helena , Micjan Michal , Donoval Martin , Kuzma Anton , Kopani Martin , Vavrinsky Erik
Published: February 2024

This article explores the importance of wearable and remote technologies in healthcare. The focus highlights its potential in continuous monitoring, examines the specificity of the issue, and offers a view of proactive healthcare. Our research describes a wide range of device types and scientific methodologies, starting from traditional chest belts to their modern alternatives and cutting-edge bioamplifiers that distinguish breathing from chest impedance variations. We also investigated innovative technologies such as the monitoring of thorax micromovements based on the principles of seismocardiography, ballistocardiography, remote camera recordings, deployment of integrated optical fibers, or extraction of respiration from cardiovascular variables. Our review is extended to include acoustic methods and breath and blood gas analysis, providing a comprehensive overview of different approaches to respiratory monitoring. The topic of monitoring respiration with wearable and remote electronics is currently the center of attention of researchers, which is also reflected by the growing number of publications. In our manuscript, we offer an overview of the most interesting ones.
Zk-SNARKs-Based Anonymous Payment Channel in Blockchain
Authors: Guo Yunwei , Liang Haochen , Zhu Liehuang , Gai Keke
Published: February 2024

Payment channels serve as an effective solution to the scalability problem of cryptocurrencies, which significantly increase transaction rates by allowing users to conduct large-scale offline transactions off-chain without posting everything to the blockchain. However, the existing payment channels lack privacy protection for the transaction amount and the linking relationship between the two parties to the transaction. Therefore, in order to address the scalability and privacy issues of cryptocurrencies such as Bitcoin, this paper proposes a zk-SNARKs-based anonymous payment channel (zk-APC), which supports an unlimited number of off-chain payments between the payer and the payee and protects the privacy of the participants. Specifically, the proposed scheme achieves relational anonymity and amount privacy for both on-chain and off-chain transactions in the payment channel through utilizing zero-knowledge proof (zk-SNARKs) and commitment schemes. This paper proves that the proposed method is more effective than similar schemes through a performance evaluation.
Connections between Parental Phubbing and Electronic Media Use in Young Children: The Mediating Role of Parent–Child Conflict and Moderating Effect of Child Emotion Regulation
Authors: Liu Xiaocen , Geng Shuliang , Lei Tong , Cheng Yan , Yu Hui
Published: February 2024

In this digital age, where parental attention is often diverted by digital engagement, the phenomenon of “parental phubbing,” defined as parents ignoring their children in favor of mobile devices, is scrutinized for its potential impact on child development. This study, utilizing questionnaire data from 612 parents and Structural Equation Modeling (SEM) with moderated mediation, examines the potential association between parental phubbing and young children’s electronic media use. The findings revealed a correlation between parental phubbing and increased electronic media use in children. Parent–child conflict, informed by instances of parental phubbing, was identified as a partial mediator in this relation. Notably, children’s emotion regulation emerged as a moderating factor, with adept regulation linked to reduced adverse effects of parental phubbing and improved relational harmony. These findings underscore the importance of parental awareness of their digital behaviors and the benefits of fostering robust parent–child relationships and supporting children’s emotional regulation to nurture well-adjusted “digital citizens” in the contemporary media landscape.
A Study of Factors Influencing Construction Workers’ Intention to Share Safety Knowledge
Authors: Mei Yujie , Huang Jianping , Liu Jianqiang , Jia Lu
Published: February 2024

There is a growing body of research on the factors influencing individual knowledge-sharing behavior, but the exploration of knowledge sharing in the construction industry is still in its infancy. Based on the theory of planned behavior (TPB), this paper introduced factors from the social exchange theory (SET) to develop a comprehensive model for exploring the intention of construction workers to share their safety knowledge. Data were collected from a total of 329 construction workers at five sites. Using the structural equation model method, the research model and path hypotheses of this study were analyzed. The results showed that altruism, trust, and reputation positively influenced the construction workers’ attitude towards sharing safety knowledge. Attitude, safety training, organizational climate, and knowledge-sharing self-efficacy could increase the construction workers’ intention to share their safety knowledge. However, the relationship between workers’ attitudes towards safety knowledge sharing and anticipated extrinsic rewards was not significant. Through identifying the factors underlying workers’ intention to share safety knowledge in the construction industry, the study helps to further understand the influencing factors and mechanisms of safety knowledge sharing willingness among the special group of construction workers and provides practical implications for engineering managers to strengthen construction safety management from the perspective of knowledge sharing.
Study of Low-Content Epoxy Asphalt Mixture Applied to the Road
Authors: Li Xiaodong , Luo Chuanxi , Wang Shaohuai , Long Xiang , Wang Yan , Li Jian , He Mu
Published: February 2024

To realize the application of epoxy asphalt concrete on roads and solve the problem of the high cost of epoxy asphalt concrete and the causes of bulging in the construction process, a low-content epoxy asphalt mixture was experimentally studied. Rheological and microscopic tests were carried out to study its performance. At the same time, 17 kinds of low-content asphalt mixture Marshall test pieces and rut plate test pieces with different oil stone ratios were made. Their road performances were tested, and a watertight breathable epoxy asphalt mixture was studied to solve the bulging problem. The research shows that, for ordinary roads, a content of epoxy resin of 10–15% can meet both the high-temperature and the low-temperature requirements. For sections with special rutting resistance requirements, a controlled epoxy resin content between 15 and 30% is recommended. When the content of epoxy resin is greater than 30%, epoxy asphalt initially forms a crosslinked spatial network. An epoxy asphalt with a complex structure from asphaltene to epoxy, with ultra-high performance, can be used for small steel bridge pavements. A BBR test showed that, with an increase in epoxy resin content, the low-temperature performance of asphalt gradually weakens. When the content was 20%, epoxy asphalt’s low-temperature performance was weaker than that of SBS-modified asphalt. Under extremely low-temperature conditions, the performance of epoxy asphalt could not meet the specifications. When the voids of low equivalent epoxy asphalt mixture are controlled at 4.1–4.5%, it is watertight and breathable; this can solve the problem of bulging and greatly reduce the cost of projects.
Research on the Reinforcement Effect and Bearing Characteristics of High-Pressure Jet-Grouting Piles on Covered Road Composite Ground in Landfill Sites
Authors: Wang Tao , Liu Xu , Liu Liyuan , Xiong Wang , Li Zhenyun
Published: February 2024

There is a notable difference between garbage pile foundations and general site foundations; due to their uneven particles, complex structure, and diverse composition, there are relatively few cases that can be used for reference. In this study, with the aim of renovating a landfill in Shenzhen, bearing-layer reinforcements were introduced in the overlying road of a garbage heap dominated by construction waste. The bearing capacity of a single-pile composite foundation was studied through a core-pulling test of high-pressure jet-grouting piles, a static load test of the bearing capacity of the single-pile composite foundation, design estimation, and numerical analysis. The results show that the obtained eigenvalue of the design estimate was 267.8 kPa, and the eigenvalue of the field test was between 182.58 kPa and 196.89 kPa, meeting the design requirement of an eigenvalue of no less than 175 kPa. The bearing capacity of the composite foundation of the single jet-grouting pile was analyzed using the ABAQUS numerical simulation software; the characteristic value of the bearing capacity of the single-pile composite foundation was 186.01 kPa, and the variation trend of its settlement–load curve was the same as that of the field test results, which met the design requirements. High-pressure jet-grouting pile technology has achieved remarkable results in the reinforcement of foundations that are mainly composed of construction waste.
A Sustainability Evaluation of Buildings: A Review on Sustainability Factors to Move towards a Greener City Environment
Authors: Park Seolah , Cho Keonhee , Choi Myeong-in
Published: February 2024

Energy-efficient and sustainable building management has always been a key concern surrounding buildings. The rise of environmental and social concern in today’s world has brought more attention to the issue of sustainable and smart building management. This paper aims to review the state-of-the-art research and performance on building management that aims to make more sustainable and energy-efficient decisions. This paper classifies building management based on technologies utilized for management and different aspects of management that should be considered when regarding the larger picture of “sustainability”. Additionally, while keeping in mind that long-term sustainability cannot be achieved through energy management alone, this research investigates previous works that also mention diverse aspects that must be taken into consideration when creating a truly successful smart building environment: costs, occupant comfort, and security. Of course, each field deserves an extensive analysis, but the purpose of this review paper is to deliver current research that has brought attention to the rapidly shifting and developing field of smart buildings to provide a macro-level holistic viewpoint on how smart buildings and homes should be approached from a sustainability viewpoint.
Deep Learning Approach on Prediction of Soil Consolidation Characteristics
Authors: Kim Mintae , Senturk Muharrem A. , Tan Rabia K. , Ordu Ertugrul , Ko Junyoung
Published: February 2024

Artificial neural network models, crucial for accurate predictions, should be meticulously designed for specific problems using deep learning-based algorithms. In this study, we compare four distinct deep learning-based artificial neural network architectures to evaluate their performance in predicting soil consolidation characteristics. The consolidation features of fine-grained soil have a significant impact on the stability of structures, particularly in terms of long-term stability. Precise prediction of soil consolidation under planned structures is vital for effective foundation design. The compression index (Cc) is an important parameter used in predicting consolidation settlement in soils. Therefore, this study examines the use of deep learning techniques, which are types of artificial neural network algorithms with deep layers, in predicting compression index (Cc) in geotechnical engineering. Four neural network models with different architectures and hyperparameters were modeled and evaluated using performance metrics such as mean absolute percentage error (MAPE), mean squared error (MSE), root mean squared error (RMSE), and coefficient of determination (R2). The dataset contains 916 samples with variables such as natural water content (w), liquid limit (LL), plasticity index (PI), and compression index (Cc). This approach allows the results of soil consolidation tests to be seen more quickly at less cost, although predictively. The findings demonstrate that deep learning models are an effective tool in predicting consolidation of fine-grained soil and offering significant opportunities for applications in geotechnical engineering. This study contributes to a more accurate prediction of soil consolidation, which is critical for the long-term stability of structural designs.
Evaluating Sustainable Colored Mortars Reinforced with Fly Ash: A Comprehensive Study on Physical and Mechanical Properties under High-Temperature Exposure
Authors: Akbulut Zehra Funda , Guler Soner , Osmanoğlu Faruk , Kıvanç Mehmet Rıza , Khan Mehran
Published: February 2024

This research primarily delves into a comprehensive investigation concerning the synergistic effects of fly ash (FA) with yellow pigment (YP) and red pigment (RP) in the workability, physical characteristics, and mechanical properties of colored mortars, both pre-and post-exposure to high temperatures. Within the experimental design, FA was employed as a 20% substitute for cement, while YP and RP were systematically incorporated into the cement mixtures at varying concentrations (1%, 3%, and 5% by weight). The specimens underwent controlled exposure to high temperatures, ranging from 300 °C to 800 °C. This study’s outcomes unveiled that while the introduction of FA positively influenced mortar workability, including YP and RP adversely impacted spreading diameters (SD), resulting in a discernible reduction in overall workability. Despite these effects, FA emerged as a pivotal factor to enhancing the residual compressive strength (RCS) and residual flexural strength (RFS) of the colored mortars. For instance, after 90 days at 800 °C, the control concrete (R0) exhibited a notable 66.13% decrease in RCS, and the sample solely incorporating FA (R1) demonstrated a reduced reduction of 55.39%. Similarly, mortars with YP additives (R2–R4) and RP additives (R5–R7) showcased RCS reductions within the range of 53.32% to 55.12% and 54.51% to 56.04%, respectively.
Suspicious Ultrasound-Occult Non-Calcified Mammographic Masses, Asymmetries, and Architectural Distortions Are Moderate Probability for Malignancy
Authors: Cohen Ethan O. , Perry Rachel E. , Legha Ravinder S. , Tso Hilda H. , Shin Kyungmin , Speer Megan E. , Phalak Kanchan A. , Sun Jia , Leung Jessica W. T.
Published: February 2024

Suspicious non-calcified mammographic findings have not been evaluated with modern mammographic technique, and the purpose of this work is to compare the likelihood of malignancy for those findings. To do this, 5018 consecutive mammographically guided biopsies performed during 2016–2019 at a large metropolitan, community-based hospital system were retrospectively reviewed. In total, 4396 were excluded for targeting calcifications, insufficient follow-up, or missing data. Thirty-seven of 126 masses (29.4%) were malignant, 44 of 194 asymmetries (22.7%) were malignant, and 77 of 302 architectural distortions (AD, 25.5%) were malignant. The combined likelihood of malignancy was 25.4%. Older age was associated with a higher likelihood of malignancy for each imaging finding type (all p ≤ 0.006), and a possible ultrasound correlation was associated with a higher likelihood of malignancy when all findings were considered together (p = 0.012). Two-view asymmetries were more frequently malignant than one-view asymmetries (p = 0.03). There were two false-negative biopsies (98.7% sensitivity and 100% specificity). In conclusion, the 25.4% likelihood of malignancy confirms the recommendation for biopsy of suspicious, ultrasound-occult, mammographic findings. Mammographically guided biopsies were highly sensitive and specific in this study. Older patient age and a possible ultrasound correlation should raise concern given the increased likelihood of malignancy in those scenarios.
Is CT Radiomics Superior to Morphological Evaluation for pN0 Characterization? A Pilot Study in Colon Cancer
Authors: Zerunian Marta , Nacci Ilaria , Caruso Damiano , Polici Michela , Masci Benedetta , De Santis Domenico , Mercantini Paolo , Arrivi Giulia , Mazzuca Federica , Paolantonio Pasquale , Pilozzi Emanuela , Vecchione Andrea , Tarallo Mariarita , Fiori Enrico , Iannicelli Elsa , Laghi Andrea
Published: February 2024

The aim of this study was to compare CT radiomics and morphological features when assessing benign lymph nodes (LNs) in colon cancer (CC). This retrospective study included 100 CC patients (test cohort) who underwent a preoperative CT examination and were diagnosed as pN0 after surgery. Regional LNs were scored with a morphological Likert scale (NODE-SCORE) and divided into two groups: low likelihood (LLM: 0–2 points) and high likelihood (HLM: 3–7 points) of malignancy. The T-test and the Mann–Whitney test were used to compare 107 radiomic features extracted from the two groups. Radiomic features were also extracted from primary lesions (PLs), and the receiver operating characteristic (ROC) was used to test a LN/PL ratio when assessing the LN’s status identified with radiomics and with the NODE-SCORE. An amount of 337 LNs were divided into 167 with LLM and 170 with HLM. Radiomics showed 15/107 features, with a significant difference (p < 0.02) between the two groups. The comparison of selected features between 81 PLs and the corresponding LNs showed all significant differences (p < 0.0001). According to the LN/PL ratio, the selected features recognized a higher number of LNs than the NODE-SCORE (p < 0.001). On validation of the cohort of 20 patients (10 pN0, 10 pN2), significant ROC curves were obtained for LN/PL busyness (AUC = 0.91; 0.69–0.99; 95% C.I.; and p < 0.001) and for LN/PL dependence entropy (AUC = 0.76; 0.52–0.92; 95% C.I.; and p = 0.03). The radiomics ratio between CC and LNs is more accurate for noninvasively discriminating benign LNs compared to CT morphological features.
Head-to-Head Comparison of [18F]PSMA-1007 and [18F]FDG PET/CT in Patients with Triple-Negative Breast Cancer
Authors: Andryszak Natalia , Świniuch Daria , Wójcik Elżbieta , Ramlau Rodryg , Ruchała Marek , Czepczyński Rafał
Published: February 2024

Background: Triple-negative breast cancer (TNBC) exhibits high aggressiveness and a notably poorer prognosis at advanced stages. Nuclear medicine offers new possibilities, not only for diagnosis but also potentially promising therapeutic strategies. This prospective study explores the potential of prostate-specific membrane antigen (PSMA) as a diagnostic and therapeutic target in TNBC. Methods: the research investigates PSMA expression in vivo among TNBC patients using [18F]PSMA-1007 PET/CT and compares it head-to-head with the standard-of-care [18F]FDG PET/CT. Results: The study involves 10 TNBC patients, revealing comparable uptake of [18F]PSMA-1007 and [18F]FDG in primary and metastatic lesions. Nodal metastases were found in eight patients, showing similar SUVmax values in both modalities. Two patients had uncountable lung metastases positive in both [18F]FDG and [18F]PSMA-1007 scans. PET-positive bone metastases were identified by 18F-PSMA in four patients, while elevated [18F]FDG uptake was found only in three of them. Distant metastases displayed higher SUVmax values in the [18F]PSMA-1007 PET/CT, as compared to [18F]FDG. Additionally, brain metastases were exclusively detected using [18F]PSMA-1007. Conclusions: the findings provide valuable insights into the expression of PSMA in TNBC and underscore the potential clinical significance of [18F]PSMA-1007 PET/CT in enhancing both diagnostic and therapeutic approaches for this aggressive breast cancer subtype.
Epithelial and Mesenchymal-like Pancreatic Cancer Cells Exhibit Different Stem Cell Phenotypes Associated with Different Metastatic Propensities
Authors: Philipp Lisa-Marie , Yesilyurt Umut-Ulas , Surrow Arne , Künstner Axel , Mehdorn Anne-Sophie , Hauser Charlotte , Gundlach Jan-Paul , Will Olga , Hoffmann Patrick , Stahmer Lea , Franzenburg Sören , Knaack Hendrike , Schumacher Udo , Busch Hauke , Sebens Susanne
Published: February 2024

Pancreatic ductal adenocarcinoma (PDAC) is mostly diagnosed at advanced or even metastasized stages, limiting the prognoses of patients. Metastasis requires high tumor cell plasticity, implying phenotypic switching in response to changing environments. Here, epithelial–mesenchymal transition (EMT), being associated with an increase in cancer stem cell (CSC) properties, and its reversion are important. Since it is poorly understood whether different CSC phenotypes exist along the EMT axis and how these impact malignancy-associated properties, we aimed to characterize CSC populations of epithelial and mesenchymal-like PDAC cells. Single-cell cloning revealed CSC (Holoclone) and non-CSC (Paraclone) clones from the PDAC cell lines Panc1 and Panc89. The Panc1 Holoclone cells showed a mesenchymal-like phenotype, dominated by a high expression of the stemness marker Nestin, while the Panc89 Holoclone cells exhibited a SOX2-dominated epithelial phenotype. The Panc89 Holoclone cells showed enhanced cell growth and a self-renewal capacity but slow cluster-like invasion. Contrarily, the Panc1 Holoclone cells showed slower cell growth and self-renewal ability but were highly invasive. Moreover, cell variants differentially responded to chemotherapy. In vivo, the Panc1 and Panc89 cell variants significantly differed regarding the number and size of metastases, as well as organ manifestation, leading to different survival outcomes. Overall, these data support the existence of different CSC phenotypes along the EMT axis in PDAC, manifesting different metastatic propensities.
Radical Resection of Malignant Tumors of Major Salivary Glands: Is This Possible?
Authors: Cantù Giulio
Published: February 2024

In primary therapy, a universally recognized surgical indication applies to all tumors of the salivary glands. According to the classic rule, radical resection of a head and neck tumor requires clean margins of at least 5 mm, although recent studies have shown that for certain locations, 1 mm may be sufficient. In the surgical resection of a tumor of the salivary glands, especially of the parotid gland, can these rules be respected? Owing to the complex branching and connections of the facial nerve within the parotid gland, even a medium-sized malignant tumor may be in contact with a branch of the nerve, thus raising the question of its preservation. The facial nerve is so important from a functional and aesthetic point of view that it is commonly believed that it should be preserved unless it is incorporated into the tumor. This is a compromise between an oncological resection, that is, the complete excision of the tumor with no residual cancer cells left behind, and quality of life. Almost all authors try to overcome this lack of radicality by indicating postoperative (chemo)radiotherapy. In this article, the pros and cons of nerve preservation will be analyzed by examining the published studies on this topic.
Artificial Intelligence for Precision Oncology of Triple-Negative Breast Cancer: Learning from Melanoma
Authors: Garrone Ornella , La Porta Caterina A. M.
Published: February 2024

Thanks to new technologies using artificial intelligence (AI) and machine learning, it is possible to use large amounts of data to try to extract information that can be used for personalized medicine. The great challenge of the future is, on the one hand, to acquire masses of biological data that nowadays are still limited and, on the other hand, to develop innovative strategies to extract information that can then be used for the development of predictive models. From this perspective, we discuss these aspects in the context of triple-negative breast cancer, a tumor where a specific treatment is still lacking and new therapies, such as immunotherapy, are under investigation. Since immunotherapy is already in use for other tumors such as melanoma, we discuss the strengths and weaknesses identified in the use of immunotherapy with melanoma to try to find more successful strategies. It is precisely in this context that AI and predictive tools can be extremely valuable. Therefore, the discoveries and advancements in immunotherapy for melanoma provide a foundation for developing effective immunotherapies for triple-negative breast cancer. Shared principles, such as immune system activation, checkpoint inhibitors, and personalized treatment, can be applied to TNBC to improve patient outcomes and offer new hope for those with aggressive, hard-to-treat breast cancer.
Outcomes and Techniques of Robotic-Assisted Partial Nephrectomy (RAPN) for Renal Hilar Masses: A Comprehensive Systematic Review
Authors: Pandolfo Savio Domenico , Wu Zhenjie , Campi Riccardo , Bertolo Riccardo , Amparore Daniele , Mari Andrea , Verze Paolo , Manfredi Celeste , Franco Antonio , Ditonno Francesco , Cerrato Clara , Ferro Matteo , Lasorsa Francesco , Contieri Roberto , Napolitano Luigi , Tufano Antonio , Lucarelli Giuseppe , Cilio Simone , Perdonà Sisto , Siracusano Salvatore , Autorino Riccardo , Aveta Achille
Published: February 2024

Background: Robot-assisted partial nephrectomy (RAPN) is increasingly being employed in the management of renal cell carcinoma (RCC) and it is expanding in the field of complex renal tumors. The aim of this systematic review was to consolidate and assess the results of RAPN when dealing with entirely central hilar masses and to examine the various methods used to address the surgical difficulties associated with them. Methods: A thorough literature search in September 2023 across various databases focused on RAPN for renal hilar masses, adhering to PRISMA guidelines. The primary goal was to evaluate RAPN’s surgical and functional outcomes, with a secondary aim of examining different surgical techniques. Out of 1250 records, 13 full-text manuscripts were reviewed. Results: Evidence is growing in favor of RAPN for renal hilar masses. Despite a predominance of retrospective studies and a lack of long-term data, RAPN shows positive surgical outcomes and preserves renal function without compromising cancer treatment effectiveness. Innovative suturing and clamping methods are emerging in surgical management. Conclusions: RAPN is a promising technique for managing renal hilar masses in RCC, offering effective surgical outcomes and renal function preservation. The study highlights the need for more long-term data and prospective studies to further validate these findings.
Hypoxia-Derived Exosomes Promote Lung Adenocarcinoma by Regulating HS3ST1-GPC4-Mediated Glycolysis
Authors: Ji Xianxiu , Zhu Ren , Gao Caixia , Xie Huikang , Gong Xiaomei , Luo Jie
Published: February 2024

Objective: The diagnosis of lung adenocarcinoma (LUAD) is often delayed due to the typically asymptomatic nature of the early-stage disease, causing advanced-stage LUAD diagnosis in most patients. Hypoxia is widely recognized as a driving force in cancer progression. Exosomes originating from hypoxic tumor cells promote tumorigenesis by influencing glycolysis, migration, invasion, and immune infiltration. Given these insights, our study aimed to explore the role of hypoxia-derived exosomal long non-coding RNA (lncRNA) OIP5-AS1 in LUAD cell lines and mouse models. Materials and Methods: Exosomes were meticulously isolated and authenticated based on their morphology and biomarkers. The interaction between heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1) and Glypican 4 (GPC4) was examined using immunoprecipitation. The influence of the hypoxia-derived exosomal lncRNA OIP5-AS1 on glycolysis was assessed in LUAD cell lines. The effect of the hypoxia-derived exosomal lncRNA OIP5-AS1 on cell proliferation and metastasis was evaluated using colony formation, cell viability, cell cycle, and apoptosis analyses. Its effects on tumor size were confirmed in xenograft animal models. Results: Our study revealed the mechanism of the hypoxia-derived exosomal lncRNA OIP5-AS1 in LUAD progression. We discovered that GPC4 promotes HS3ST1-mediated glycolysis and that the hypoxia-derived exosomal lncRNA OIP5-AS1 enhances glycolysis by regulating miR-200c-3p in LUAD cells. Notably, this lncRNA stimulates LUAD cell proliferation and metastasis and fosters LUAD tumor size via miR-200c-3p. Our findings underscore the potential role of the hypoxia-derived exosomal lncRNA OIP5-AS1 in LUAD progression. Conclusions: The hypoxia-derived exosomal lncRNA OIP5-AS1 promotes LUAD by regulating HS3ST1-GPC4-mediated glycolysis via miR-200c-3p.