bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the PI3K/Akt/PTEN/mTOR Network
Authors: Almaimani Riyad Adnan , Aslam Akhmed , Ahmad Jawwad , El-Readi Mahmoud Zaki , El-Boshy Mohamed E. , Abdelghany Abdelghany H. , Idris Shakir , Alhadrami Mai , Althubiti Mohammad , Almasmoum Hussain A. , Ghaith Mazen M. , Elzubeir Mohamed E. , Eid Safaa Yehia , Refaat Bassem
Published: March 2022

Chemoresistance to 5-fluorouracil (5-FU) is common during colorectal cancer (CRC) treatment. This study measured the chemotherapeutic effects of 5-FU, active vitamin D3 (VD3), and/or metformin single/dual/triple regimens as complementary/alternative therapies. Ninety male mice were divided into: negative and positive (PC) controls, and 5-FU, VD3, Met, 5-FU/VD3, 5-FU/Met, VD3/Met, and 5-FU/VD3/Met groups. Treatments lasted four weeks following CRC induction by azoxymethane. Similar regimens were also applied in the SW480 and SW620 CRC cell lines. The PC mice had abundant tumours, markedly elevated proliferation markers (survivin/CCND1) and PI3K/Akt/mTOR, and reduced p21/PTEN/cytochrome C/caspase-3 and apoptosis. All therapies reduced tumour numbers, with 5-FU/VD3/Met being the most efficacious regimen. All protocols decreased cell proliferation markers, inhibited PI3K/Akt/mTOR molecules, and increased proapoptotic molecules with an apoptosis index, and 5-FU/VD3/Met revealed the strongest effects. In vitro, all therapies equally induced G1 phase arrest in SW480 cells, whereas metformin-alone showed maximal SW620 cell numbers in the G0/G1 phase. 5-FU/Met co-therapy also showed the highest apoptotic SW480 cell numbers (13%), whilst 5-FU/VD3/Met disclosed the lowest viable SW620 cell percentages (81%). Moreover, 5-FU/VD3/Met revealed maximal inhibitions of cell cycle inducers (CCND1/CCND3), cell survival (BCL2), and the PI3K/Akt/mTOR molecules alongside the highest expression of cell cycle inhibitors (p21/p27), proapoptotic markers (BAX/cytochrome C/caspase-3), and PTEN in both cell lines. In conclusion, metformin monotherapy was superior to VD3, whereas the 5-FU/Met protocol showed better anticancer effects relative to the other dual therapies. However, the 5-FU/VD3/Met approach displayed the best in vivo and in vitro tumoricidal effects related to cell cycle arrest and apoptosis, justifiably by enhanced modulations of the PI3K/PTEN/Akt/mTOR pathway.
Effect of Osimertinib on CTCs and ctDNA in EGFR Mutant Non-Small Cell Lung Cancer Patients: The Prognostic Relevance of Liquid Biopsy
Authors: Kallergi Galatea , Kontopodis Emmanouil , Ntzifa Aliki , Jordana-Ariza Núria , Karachaliou Niki , Pantazaka Evangelia , Charalambous Haris A. , Psyrri Amanda , Tsaroucha Emily , Boukovinas Ioannis , Koumarianou Anna , Hatzidaki Dora , Lianidou Evi , Georgoulias Vassilis , Rosell Rafael , Kotsakis Athanasios
Published: March 2022

Introduction: Liquid biopsy is a useful tool for monitoring treatment outcome in solid tumors, including lung cancer. The relevance of monitoring CTCs and plasma ctDNA as predictors of clinical outcome was assessed in EGFR-mutant NSCLC patients treated with osimertinib. Methods: Forty-seven EGFR-mutant NSCLC patients who had progressed on prior first- or second-generation EGFR inhibitors were enrolled in the study and treated with osimertinib, irrespective of the presence of the T790M mutation in the primary tumor or the plasma. Peripheral blood was collected at baseline (n = 47), post-Cycle 1 (n = 47), and at the end of treatment (EOT; n = 39). CTCs were evaluated in 32 patients at the same time points (n = 32, n = 27, and n = 21, respectively) and phenotypic characterization was performed using triple immunofluorescence staining (CK/VIM/CD45). Results: Osimertinib resulted in an ORR of 34% (2 CR) and a DCR of 76.6%. The median PFS and OS values were 7.5 (range, 0.8–52.8) and 15.1 (range, 2.1–52.8) months, respectively. ctDNA was detected in 61.7%, 27.7%, and 61.5% of patients at baseline, post-Cycle 1, and EOT, respectively. CTCs (CK+/CD45-) were detected in 68.8%, 48.1%, and 61.9% of patients at the three time points, respectively. CTCs expressing both epithelial and mesenchymal markers (CK+/VIM+/CD45-) were detected in 56.3% and 29.6% of patients at baseline and post-Cycle 1, respectively. The detection of ctDNA at baseline and post-Cycle 1 was associated with shorter PFS and OS, whereas the ctDNA clearance post-Cycle 1 resulted in a significantly longer PFS and OS. Multivariate analysis revealed that male sex and the detection of ctDNA at baseline were independent predictors of shorter PFS (HR: 2.6, 95% C.I.: 1.2–5.5, p = 0.015 and HR: 3.0, 95% C.I.: 1.3–6.9; p = 0.009, respectively). Conclusions: The decrease in both CTCs and ctDNA occurring early during osimertinib treatment is predictive of better outcome, implying that liquid biopsy monitoring may be a valuable tool for the assessment of treatment efficacy.
Circadian and Immunity Cycle Talk in Cancer Destination: From Biological Aspects to In Silico Analysis
Authors: Mirian Mina , Hariri Amirali , Yadollahi Mahtasadat , Kohandel Mohammad
Published: March 2022

Cancer is the leading cause of death and a major problem to increasing life expectancy worldwide. In recent years, various approaches such as surgery, chemotherapy, radiation, targeted therapies, and the newest pillar, immunotherapy, have been developed to treat cancer. Among key factors impacting the effectiveness of treatment, the administration of drugs based on the circadian rhythm in a person and within individuals can significantly elevate drug efficacy, reduce adverse effects, and prevent drug resistance. Circadian clocks also affect various physiological processes such as the sleep cycle, body temperature cycle, digestive and cardiovascular processes, and endocrine and immune systems. In recent years, to achieve precision patterns for drug administration using computational methods, the interaction of the effects of drugs and their cellular pathways has been considered more seriously. Integrated data-derived pathological images and genomics, transcriptomics, and proteomics analyses have provided an understanding of the molecular basis of cancer and dramatically revealed interactions between circadian and immunity cycles. Here, we describe crosstalk between the circadian cycle signaling pathway and immunity cycle in cancer and discuss how tumor microenvironment affects the influence on treatment process based on individuals’ genetic differences. Moreover, we highlight recent advances in computational modeling that pave the way for personalized immune chronotherapy.
Vulvar Cancer: Facing a Rare Disease
Authors: Preti Mario , Querleu Denis
Published: March 2022

“We must never be afraid to go too far, for truth lies beyond [...]
Prometastatic Effect of ATX Derived from Alveolar Type II Pneumocytes and B16-F10 Melanoma Cells
Authors: Dacheux Mélanie A. , Lee Sue Chin , Shin Yoojin , Norman Derek D. , Lin Kuan-Hung , E Shuyu , Yue Junming , Benyó Zoltán , Tigyi Gábor J.
Published: March 2022

Although metastases are the principal cause of cancer-related deaths, the molecular aspects of the role of stromal cells in the establishment of the metastatic niche remain poorly understood. One of the most prevalent sites for cancer metastasis is the lungs. According to recent research, lung stromal cells such as bronchial epithelial cells and resident macrophages secrete autotaxin (ATX), an enzyme with lysophospholipase D activity that promotes cancer progression. In fact, several studies have shown that many cell types in the lung stroma could provide a rich source of ATX in diseases. In the present study, we sought to determine whether ATX derived from alveolar type II epithelial (ATII) pneumocytes could modulate the progression of lung metastasis, which has not been evaluated previously. To accomplish this, we used the B16-F10 syngeneic melanoma model, which readily metastasizes to the lungs when injected intravenously. Because B16-F10 cells express high levels of ATX, we used the CRISPR-Cas9 technology to knock out the ATX gene in B16-F10 cells, eliminating the contribution of tumor-derived ATX in lung metastasis. Next, we used the inducible Cre/loxP system (Sftpc-CreERT2/Enpp2fl/fl) to generate conditional knockout (KO) mice in which ATX is specifically deleted in ATII cells (i.e., Sftpc-KO). Injection of ATX-KO B16-F10 cells into Sftpc-KO or Sftpc-WT control littermates allowed us to investigate the specific contribution of ATII-derived ATX in lung metastasis. We found that targeted KO of ATX in ATII cells significantly reduced the metastatic burden of ATX-KO B16-F10 cells by 30% (unpaired t-test, p = 0.028) compared to Sftpc-WT control mice, suggesting that ATX derived from ATII cells could affect the metastatic progression. We detected upregulated levels of cytokines such as IFNγ (unpaired t-test, p < 0.0001) and TNFα (unpaired t-test, p = 0.0003), which could favor the increase in infiltrating CD8+ T cells observed in the tumor regions of Sftpc-KO mice. Taken together, our results highlight the contribution of host ATII cells as a stromal source of ATX in the progression of melanoma lung metastasis.
The Barcelona Predictive Model of Clinically Significant Prostate Cancer
Authors: Morote Juan , Borque-Fernando Angel , Triquell Marina , Celma Anna , Regis Lucas , Escobar Manel , Mast Richard , de Torres Inés M. , Semidey María E. , Abascal José M. , Sola Carles , Servian Pol , Salvador Daniel , Santamaría Anna , Planas Jacques , Esteban Luis M. , Trilla Enrique
Published: March 2022

A new and externally validated MRI-PM for csPCa was developed in the metropolitan area of Barcelona, and a web-RC designed with the new option of selecting the csPCa probability threshold. The development cohort comprised 1486 men scheduled to undergo a 3-tesla multiparametric MRI (mpMRI) and guided and/or systematic biopsies in one academic institution of Barcelona. The external validation cohort comprised 946 men in whom the same diagnostic approach was carried out as in the development cohort, in two other academic institutions of the same metropolitan area. CsPCa was detected in 36.9% of men in the development cohort and 40.8% in the external validation cohort (p = 0.054). The area under the curve of mpMRI increased from 0.842 to 0.897 in the developed MRI-PM (p < 0.001), and from 0.743 to 0.858 in the external validation cohort (p < 0.001). A selected 15% threshold avoided 40.1% of prostate biopsies and missed 5.4% of the 36.9% csPCa detected in the development cohort. In men with PI-RADS <3, 4.3% would be biopsied and 32.3% of all existing 4.2% of csPCa would be detected. In men with PI-RADS 3, 62% of prostate biopsies would be avoided and 28% of all existing 12.4% of csPCa would be undetected. In men with PI-RADS 4, 4% of prostate biopsies would be avoided and 0.6% of all existing 43.1% of csPCa would be undetected. In men with PI-RADS 5, 0.6% of prostate biopsies would be avoided and none of the existing 42.0% of csPCa would be undetected. The Barcelona MRI-PM presented good performance on the overall population; however, its clinical usefulness varied regarding the PI-RADS category. The selection of csPCa probability thresholds in the designed RC may facilitate external validation and outperformance of MRI-PMs in specific PI-RADS categories.
Heterogeneous Photodegradation for the Abatement of Recalcitrant COD in Synthetic Tanning Wastewater
Authors: Toscanesi Maria , Russo Vincenzo , Medici Antonio , Giarra Antonella , Hmoudah Maryam , Di Serio Martino , Trifuoggi Marco
Published: March 2022

Tannery wastewater is considered one of the most contaminated and problematic wastes since it consists of considerable amounts of organic and inorganic compounds. These contaminants result in high chemical oxygen demand (COD), biochemical oxygen demand (BOD), and total suspended solids (TSS). In this work, the heterogeneous photodegradation of recalcitrant COD in wastewater from the tanning industry was investigated, in particular the recalcitrant COD due to the presence of vegetable tannins extracted from mimosa and chestnut and from synthetic tannins based on 4,4′ dihydroxy phenyl sulfone. TiO2 Aeroxide P-25 was employed to study the photodegradation of model molecules in batch conditions under different parameters, namely initial concentration of COD, temperature, and catalyst dose. The maximum COD abatement reached was 60%. Additionally, preliminary kinetic investigation was conducted to derive the main kinetic parameters that can be useful for process scale-up. It was found to be independent of the temperature value but linearly dependent on both catalyst loading and the initial COD value.
The Effect of Thin Film Fabrication Techniques on the Performance of rGO Based NO2 Gas Sensors at Room Temperature
Authors: Shahzad Shazrah , Wang Huaipeng , Li Weiwei , Sun Yilin , Xie Dan , Ren Tianling
Published: March 2022

Reduced graphene oxide (rGO) has attracted enormous interest as a promising candidate material for gas detection due to its large specific surface areas. In our work, rGO films were fabricated on a large scale using dip-coating and spin-coating methods for the detection of nitrogen dioxide (NO2) gas at room temperature. The influence of different test environments on the sensing performance, including the test atmosphere, gas flow and gas pressure was evaluated. The response time of the dip-coating method was 573 s with a long recovery period of 639 s and for the spin-coating method, the response time and recovery time was 386 s and 577 s, respectively. In addition, the spin-coated sensor exhibited high selectivity to NO2, with the response increasing by more than 20% (for 15 ppm NO2) as compared with that for HCHO, NH3, and CH4. Our results indicated that the spin coating method was more suitable for rGO-based gas sensors with higher performance.
Optical Response of CVD-Grown ML-WS2 Flakes on an Ultra-Dense Au NP Plasmonic Array
Authors: Ferrera Marzia , Ramò Lorenzo , Convertino Domenica , Orlandini Giorgio , Pace Simona , Milekhin Ilya , Magnozzi Michele , Rahaman Mahfujur , Zahn Dietrich R. T. , Coletti Camilla , Canepa Maurizio , Bisio Francesco
Published: March 2022

The combination of metallic nanostructures with two-dimensional transition metal dichalcogenides is an efficient way to make the optical properties of the latter more appealing for opto-electronic applications. In this work, we investigate the optical properties of monolayer WS2 flakes grown by chemical vapour deposition and transferred onto a densely-packed array of plasmonic Au nanoparticles (NPs). The optical response was measured as a function of the thickness of a dielectric spacer intercalated between the two materials and of the system temperature, in the 75–350 K range. We show that a weak interaction is established between WS2 and Au NPs, leading to temperature- and spacer-thickness-dependent coupling between the localized surface plasmon resonance of Au NPs and the WS2 exciton. We suggest that the closely-packed morphology of the plasmonic array promotes a high confinement of the electromagnetic field in regions inaccessible by the WS2 deposited on top. This allows the achievement of direct contact between WS2 and Au while preserving a strong connotation of the properties of the two materials also in the hybrid system.
Is Obesity a Risk Factor for Loss of Reduction in Children with Distal Radius Fractures Treated Conservatively?
Authors: Vescio Andrea , Testa Gianluca , Sapienza Marco , Caldaci Alessia , Montemagno Marco , Andreacchio Antonio , Canavese Federico , Pavone Vito
Published: March 2022

Background: Obesity in children is a clinical and social burden. The distal radius (DR) is the most common site of fractures in childhood and conservative treatment is widely used. Loss of reduction (LOR) is the major casting complication. The aim of this study is to evaluate obesity as a risk factor for LOR in children with displaced DR fractures (DRF) treated conservatively. Methods: 189 children under 16 years of age were treated conservatively for DRF. Patients were divided into three groups: normal weight (NW), overweight (OW) and obese (OB). The following radiographic criteria were evaluated in all patients: amount of initial translation (IT); quality of initial reduction; Cast (CI), Padding (PI), Canterbury (CaI), Gap (GI) and Three-Points (3PI) indices and the presence of LOR. Results: Statistically significant differences were found between the NW and the OB group for number of LOR (p = 0.002), severity (grade) of initial translation (p = 0.008), quality of initial reduction (p = 0.01) as well as CsI and CaI (p < 0.001). Conclusions: Obese children have a significantly higher rate of LOR compared to NW and OW children. A close follow-up is necessary in this population of patients. Preventive percutaneous pinning could be considered in older obese patients in order to reduce the need for further treatment.
Short Term Radiological Outcome of Combined Femoral and Ilium Osteotomy in Pelvic Reconstruction of the Child
Authors: Pisecky Lorenz , Großbötzl Gerhard , Stevoska Stella , Klotz Matthias Christoph Michael , Haas Christina , Gotterbarm Tobias , Luger Matthias , Gahleitner Manuel
Published: March 2022

Background and Objectives: Reconstruction of the pelvic joint is a common way to address developmental dysplasia of the hip (DDH), as well as neurogenic dislocation of the hip (NDH) and Legg–Calvé–Perthes disease (LCPD) in children. The purpose of this study was to analyze the short-term radiologic outcome after hip reconstructive surgery either treated with sole osteotomy of the femur or in combination with iliac osteotomy in patients with DDH, NDH and LCPD. Materials and Methods: X-rays of 73 children, aged 2–18 years, with DDH, NDH and LCPD after hip reconstructive surgery were measured retrospectively and compared to the preoperative x-rays concerning various parameters to define hip geometry. The surgical procedures were femoral osteotomy (74), Salter innominate osteotomy (27), Pemberton osteotomy (27), open reduction (37), Chiari osteotomy (4). The pre-/postoperative acetabular index (AI), center-edge angle (CE) and Reimers migration index (RMI) were evaluated before and 3 months after surgery. Results: Hip geometry parameters improved significantly (RMI: preop/postop: 62.23% ± 31.63%/6.30% ± 11.51%, p < 0.001; CE: 11.53° ± 20.16°/30.58 ± 8.81°, p < 0.001; AI: 28.67° ± 9.2°/19.17 ± 7.65°, p < 0.001). Sub-group analysis showed a superior RMI in DDH compared with NDH 3 months after surgery (DDH/NDH: 2.77% ± 6.9%/12.94% ± 13.5%; p = 0.011). Osteotomy of the iliac bone (Salter innominate, Pemberton, Chiari) resulted in a significant improvement of the postoperative RMI compared to cases without osteotomy of the ilium (7.02 ± 11.1% vs. 16.85 ± 4.71%; p = 0.035). Conclusions: Femoral and pelvic osteotomies are effective to improve the radiological pelvic parameters in infants and adolescents with DDH, NDH and LCPD. In addition, the study found that the combination of femoral and pelvic osteotomy led to a better RMI than femoral osteotomy alone. Using the combined ilium and femoral osteotomy, it was possible to show the highest effect on correction of the hip geometry with respect to residual RMI.
Multigene Panel Sequencing Reveals Cancer-Specific and Common Somatic Mutations in Colorectal Cancer Patients: An Egyptian Experience
Authors: Youssef Amira Salah El-Din , Abdel-Fattah Mohamed A. , Lotfy Mai M. , Nassar Auhood , Abouelhoda Mohamed , Touny Ahmed O. , Hassan Zeinab K. , Mohey Eldin Mohammed , Bahnassy Abeer A. , Khaled Hussein , Zekri Abdel Rahman N.
Published: March 2022

This study aims at identifying common pathogenic somatic mutations at different stages of colorectal carcinogenesis in Egyptian patients. Our cohort included colonoscopic biopsies collected from 120 patients: 20 biopsies from patients with inflammatory bowel disease, 38 from colonic polyp patients, and 62 from patients with colorectal cancer. On top of this, the cohort included 20 biopsies from patients with non-specific mild to moderated colitis. Targeted DNA sequencing using a customized gene panel of 96 colorectal related genes running on the Ion Torrent NGS technology was used to process the samples. Our results revealed that 69% of all cases harbored at least one somatic mutation. Fifty-seven genes were found to carry 232 somatic non-synonymous variants. The most frequently pathogenic somatic mutations were localized in TP53, APC, KRAS, and PIK3CA. In total, 16 somatic mutations were detected in the CRC group and in either the IBD or CP group. In addition, our data showed that 51% of total somatic variants were CRC-specific variants. The average number of CRC-specific variants per sample is 2.4. The top genes carrying CRC-specific mutations are APC, TP53, PIK3CA, FBXW7, ATM, and SMAD4. It seems obvious that TP53 and APC genes were the most affected genes with somatic mutations in all groups. Of interest, 85% and 28% of the APC and TP53 deleterious somatic mutations were located in Exon 14 and Exon 3, respectively. Besides, 37% and 28% of the total somatic mutations identified in APC and TP53 were CRC-specific variants, respectively. Moreover, we identified that, in 29 somatic mutations in 21 genes, their association with CRC patients was unprecedented. Ten detected variants were likely to be novel: six in PIK3CA and four variants in FBXW7. The detected P53, Wnt/βcatenin, Angiogenesis, EGFR, TGF-β and Interleukin signaling pathways were the most altered pathways in 22%, 16%, 12%, 10%, 9% and 9% of the CRC patients, respectively. These results would contribute to a better understanding of the colorectal cancer and in introducing personalized therapies for Egyptian CRC patients.
Cyclin D1 Serves as a Poor Prognostic Biomarker in Stage I Gastric Cancer
Authors: Go Se-Il , Ko Gyung Hyuck , Lee Won Sup , Lee Jeong-Hee , Jeong Sang-Ho , Lee Young-Joon , Hong Soon Chan , Ha Woo Song
Published: March 2022

TNM stage still serves as the best prognostic marker in gastric cancer (GC). The next step is to find prognostic biomarkers that detect subgroups with different prognoses in the same TNM stage. In this study, the expression levels of epidermal growth factor receptor (EGFR) and cyclin D1 were assessed in 96 tissue samples, including non-tumorous tissue, adenoma, and carcinoma. Then, the prognostic impact of EGFR and cyclin D1 was retrospectively investigated in 316 patients who underwent R0 resection for GC. EGFR positivity increased as gastric tissue became malignant, and cyclin D1 positivity was increased in all the tumorous tissues. However, there was no survival difference caused by the EGFR positivity, while the cyclin D1-postive group had worse overall survival (OS) than the cyclin D1-negative group in stage I GC (10-year survival rate (10-YSR): 62.8% vs. 86.5%, p = 0.010). In subgroup analyses for the propensity score-matched (PSM) cohort, there were also significant differences in the OS according to the cyclin D1 positivity in stage I GC but not in stage II and III GC. Upon multivariate analysis, cyclin D1 positivity was an independent prognostic factor in stage I GC. In conclusion, cyclin D1 may be a useful biomarker for predicting prognosis in stage I GC.
Evaluation of the Prediction and Durability on the Chloride Penetration in Cementitious Materials with Blast Furnace Slag as Cement Addition
Authors: Subpa-asa Prang , Nito Nobukazu , Fujiwara Satoshi , Date Shigeyuki
Published: March 2022

Blast furnace slag (BFS) is a mortar additive in which the utilization of varied curing conditions and the basicity of BFS determine the fineness of the resulting mortar and, thereby, its salt prevention properties. This study evaluates and compares the salt-prevention properties of mortar prepared by either steam curing or water curing. The physical properties, for example, the BFS fineness, revealed the factors significantly affected by basicity that influence the salt-preventive properties of mortar in the specimens examined, such as the lead time and diffusion coefficient. Furthermore, these factors were also significantly affected by differences in curing conditions and other physical properties. However, few studies have examined its use in reducing chloride ion permeability as the main factor of corrosion reactions. Thus, this study evaluates specific surface, water/binder ratio (W/B), and curing conditions on the chloride penetration in cementitious materials with blast furnace slag as cement addition in terms of delaying chloride ion penetration, which affects corrosion reactions. Results of the study are intended to guide development of products for use in the precast concrete industry, toward extending the life of concrete structures, especially reinforced concrete structures in marine environments. In addition, the resulting durability measurements from the experiment conducted are illustrated. This study indicates that differences in Blaine size properties significantly influence water curing. Furthermore, results reveal the effects of combining BFS with various Blaine values and ratio-affecting properties on mortar. In conclusion, concrete materials that decrease durability against chloride attack and improve mechanical properties for precast manufacturer industrial applications are successfully developed in this study. In addition, the use of water-curing conditions, high Blaine value, high cement replacement ratio, and W/B tend to improve the general mechanical property performance and durability against chloride ion attack.
The Use of a Real-Time COVID-19 Standalone Device in an Emergency Department of a Tertiary Hospital in Singapore: A Pilot Observational Study
Authors: Joshi Rhea , Ng Ee , Singh Rahul , Kuan Win , Daruwalla Zubin
Published: March 2022

This study describes the implementation and utility of a standalone device designed, developed, and 3D-printed by PwC Singapore and Southeast Asia Consulting as a response to Corona Virus Disease 2019 (COVID-19), in the Emergency Department (ED) of the National University Hospital in Singapore. Over a 2-week period, all staff used the devices for the duration of their shifts, with the device additionally tagged to patients who were swabbed on suspicion of or surveillance for COVID-19 in the subsequent two weeks. Additional control hardware was placed in the ED to analyze (1) time-intervals of greatest interaction, (2) clusters of close physical distance among staff, (3) areas with high traffic, and (4) potential use of a rapid contact tracing capability. Time-day trends indicated the greatest interaction time-intervals during the beginning of the day, with Monday hosting the greatest average daily interactions across the first two weeks. Social cluster trends indicated the greatest average daily interactions between nurses–nurses during Phase 1, and patients–patients during Phase 2. User-location trends revealed the greatest average daily interaction counts at the intermediate care areas, isolation outdoor tent, pantry, and isolation holding units relative to other areas. Individual-level visualization and contact tracing capabilities were not utilized as nobody contracted COVID-19 during either phase. While congregation in intermediate and resuscitation areas are unavoidable within the ED context, the findings of this study were acted upon, improving social distancing within the pantry and between healthcare groups. This real-time solution addresses multiple privacy concerns while rapidly facilitating contact tracing.
Reproductive Ecology of Distylous Shoreside Polygonum criopolitanum Hance
Authors: Chen Ming-Lin , Qi Meng-Ying , Bai Bei-Bei , Han Xue
Published: March 2022

In this study, distyly was clearly confirmed in Polygonum criopolitanum Hance, which exhibited strict self-incompatibility. Unlike other distylous species, style-morph ratios of P. criopolitanum often deviated obviously from 1:1, and many populations were solely composed of long or short stylous flowers; the 1:1 style-morph ratio was occasionally found in very large populations. P. criopolitanum was dimorphic for intrinsic features such as style height and anther height and ancillary features such as pollen size and number. The L-morph flowers produced a significantly smaller and higher number of pollen grains than the S-morph flowers, and the stigma papillae of both morphs were not significantly different. We nearly found no seed sets in most wild populations and very low seed sets occasionally occurred in large populations, which was different from other species of Polygonaceae. Mating experiments showed that P. criopolitanum has a strict self-incompatibility system and clonal propagation was more common than sexual propagation, which was adaptive with the unisexual wild populations. Hygrocolous habitat, 20–60% soil water content, and height gap less than 4 m to the adjacent water were the main limiting factors for the distribution of P. criopolitanum.
Transcriptomic Response of Human Nosocomial Pathogen Pseudomonas aeruginosa Biofilms Following Continuous Exposure to Antibiotic-Impregnated Catheters
Authors: Sung Kidon , Li Dan , Chon Jungwhan , Kweon Ohgew , Kim Minjae , Xu Joshua , Park Miseon , Khan Saeed A.
Published: March 2022

Biofilms are complex surface-attached bacterial communities that serve as a protective survival strategy to adapt to an environment. Bacterial contamination and biofilm formation on implantable medical devices pose a serious threat to human health, and these biofilms have become the most important source of nosocomial infections. Although antimicrobial-impregnated catheters have been employed to prevent bacterial infection, there have been concerns about the potential emergence of antibiotic resistance. To investigate the risk of developing resistance, we performed RNA-sequencing gene expression profiling of P. aeruginosa biofilms in response to chronic exposure to clindamycin and rifampicin eluted from antibiotic-coated catheters in a CDC biofilm bioreactor. There were 877 and 178 differentially expressed genes identified in planktonic and biofilm cells after growth for 144 h with control (without antibiotic-impregnation) and clindamycin/rifampicin-impregnated catheters, respectively. The differentially expressed genes were further analyzed by Clusters of Orthologous Groups (COGs) functional classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The data are publicly available through the GEO database with accession number GSE153546.
Towards a National-Scale Dataset of Geotechnical and Hydrological Soil Parameters for Shallow Landslide Modeling
Authors: Vannocci Pietro , Segoni Samuele , Masi Elena Benedetta , Cardi Francesco , Nocentini Nicola , Rosi Ascanio , Bicocchi Gabriele , D’Ambrosio Michele , Nocentini Massimiliano , Lombardi Luca , Tofani Veronica , Casagli Nicola , Catani Filippo
Published: March 2022

One of the main constraints in assessing shallow landslide hazards through physically based models is the need to characterize the geotechnical parameters of the involved materials. Indeed, the quantity and quality of input data are closely related to the reliability of the results of every model used, therefore data acquisition is a critical and time-consuming step in every research activity. In this perspective, we reviewed all official certificates of tests performed through 30 years at the Geotechnics Laboratory of the Earth Science Department (University of Firenze, Firenze, Italy), compiling a dataset in which 380 points are accurately geolocated and provide information about one or more geotechnical parameters used in slope stability modeling. All tests performed in the past (in the framework of previous research programs, agreements of cooperation, or to support didactic activities) were gathered, homogenized, digitalized, and geotagged. The dataset is based on both on-site tests and laboratory tests, it accounts for 40 attributes, among which 13 are descriptive (e.g., lithology or location) and 27 may be of direct interest in slope stability modeling as input parameters. The dataset is made openly available and can be useful for scientists or practitioners committed to landslide modeling.
CNN-Based Dense Monocular Visual SLAM for Real-Time UAV Exploration in Emergency Conditions
Authors: Steenbeek Anne , Nex Francesco
Published: March 2022

Unmanned Aerial Vehicles (UAVs) for 3D indoor mapping applications are often equipped with bulky and expensive sensors, such as LIDAR (Light Detection and Ranging) or depth cameras. The same task could be also performed by inexpensive RGB cameras installed on light and small platforms that are more agile to move in confined spaces, such as during emergencies. However, this task is still challenging because of the absence of a GNSS (Global Navigation Satellite System) signal that limits the localization (and scaling) of the UAV. The reduced density of points in feature-based monocular SLAM (Simultaneous Localization and Mapping) then limits the completeness of the delivered maps. In this paper, the real-time capabilities of a commercial, inexpensive UAV (DJI Tello) for indoor mapping are investigated. The work aims to assess its suitability for quick mapping in emergency conditions to support First Responders (FR) during rescue operations in collapsed buildings. The proposed solution only uses images in input and integrates SLAM and CNN-based (Convolutional Neural Networks) Single Image Depth Estimation (SIDE) algorithms to densify and scale the data and to deliver a map of the environment suitable for real-time exploration. The implemented algorithms, the training strategy of the network, and the first tests on the main elements of the proposed methodology are reported in detail. The results achieved in real indoor environments are also presented, demonstrating performances that are compatible with FRs’ requirements to explore indoor volumes before entering the building.
Digitalisation in Hospitals in COVID-19 Times—A Case Study of the Czech Republic
Authors: Zimmermannova Jarmila , Pavlik Lukas , Chytilova Ekaterina
Published: March 2022

In COVID-19 times, the healthcare system needs more financial and other resources for covering all necessary medical products and services. On the other hand, we have observed pressure on the effectiveness and optimisation of resources in hospitals and healthcare facilities. Digitalisation represents an important source of information for various levels of management in hospitals. The main aim of our research is the identification of the benefits of digitalisation of medical devices in hospitals in COVID-19 times, focusing on a case study of the Czech Republic. For our methodological approach, a literature review, data analysis, correlation analysis, and regression analysis were used. The case study presents the changes to the equipment/facilities use in years 2019 and 2020 in a selected hospital in Prague and the impact of COVID-19 on such use of resources. Management and financial issues are discussed, together with recommendations for healthcare sector management. As a result, economic benefits are represented mainly by various kinds of savings and optimisation of both processes and employees. On the other hand, it is not easy to identify all possible savings, as some of them can be in non-financial expression.
Low-Cost Data Acquisition System for Solar Thermal Collectors
Authors: Panagopoulos Orestis , Argiriou Athanassios A.
Published: March 2022

Solar thermal collectors are among the most popular renewable energy research subjects. Automatic Data Acquisition Systems (ADAS) have greatly facilitated their experimental testing, but their high cost is a drawback. In this paper, we present the design and testing of a decentralized, low-cost alternative ADAS based on the ESP32 microcontroller and on open-source software. The proposed system can be used for the experimental characterization of water (or air) operated solar thermal collectors in accordance with the ISO 9806:2017 requirements, but it is also compatible with sensors with lower specifications. We present also its performance results when applied for the testing of a solar thermal collector.
Approximate Entropy of Spiking Series Reveals Different Dynamical States in Cortical Assemblies
Authors: Ermini Leonardo , Massobrio Paolo , Mesin Luca
Published: March 2022

Self-organized criticality theory proved that information transmission and computational performances of neural networks are optimal in critical state. By using recordings of the spontaneous activity originated by dissociated neuronal assemblies coupled to Micro-Electrode Arrays (MEAs), we tested this hypothesis using Approximate Entropy (ApEn) as a measure of complexity and information transfer. We analysed 60 min of electrophysiological activity of three neuronal cultures exhibiting either sub-critical, critical or super-critical behaviour. The firing patterns on each electrode was studied in terms of the inter-spike interval (ISI), whose complexity was quantified using ApEn. We assessed that in critical state the local complexity (measured in terms of ApEn) is larger than in sub- and super-critical conditions (mean ± std, ApEn about 0.93 ± 0.09, 0.66 ± 0.18, 0.49 ± 0.27, for the cultures in critical, sub-critical and super-critical state, respectively—differences statistically significant). Our estimations were stable when considering epochs as short as 5 min (pairwise cross-correlation of spatial distribution of mean ApEn of 94 ± 5%). These preliminary results indicate that ApEn has the potential of being a reliable and stable index to monitor local information transmission in a neuronal network during maturation. Thus, ApEn applied on ISI time series appears to be potentially useful to reflect the overall complex behaviour of the neural network, even monitoring a single specific location.
AlexNet Convolutional Neural Network for Disease Detection and Classification of Tomato Leaf
Authors: Chen Hsing-Chung , Widodo Agung Mulyo , Wisnujati Andika , Rahaman Mosiur , Lin Jerry Chun-Wei , Chen Liukui , Weng Chien-Erh
Published: March 2022

With limited retrieval of reserves and restricted capability in plant pathology, automation of processes becomes essential. All over the world, farmers are struggling to prevent various harm from bacteria or pathogens such as viruses, fungi, worms, protozoa, and insects. Deep learning is currently widely used across a wide range of applications, including desktop, web, and mobile. In this study, the authors attempt to implement the function of AlexNet modification architecture-based CNN on the Android platform to predict tomato diseases based on leaf image. A dataset with of 18,345 training data and 4,585 testing data was used to create the predictive model. The information is separated into ten labels for tomato leaf diseases, each with 64 × 64 RGB pixels. The best model using the Adam optimizer with a realizing rate of 0.0005, the number of epochs 75, batch size 128, and an uncompromising cross-entropy loss function, has a high model accuracy with an average of 98%, a strictness rate of 0.98, a recall value of 0.99, and an F1-count of 0.98 with a loss of 0.1331, so that the classification results are good and very precise.
Harvesting Systems for RF Energy: Trends, Challenges, Techniques, and Tradeoffs
Authors: Muhammad Surajo , Tiang Jun Jiat , Wong Sew Kin , Rambe Ali H. , Adam Ismahayati , Smida Amor , Waly Mohamed Ibrahim , Iqbal Amjad , Abubakar Adamu Saidu , Mohd Yasin Mohd Najib
Published: March 2022

The RFEH design challenges can be broadly classified into overall radio frequency direct current (RF-to-DC) power conversion efficiency (PCE), form factor, operational bandwidth (BW), and compactness. A detailed overview of the essential components of an RFEH system is presented in this paper. Various design approaches have been proposed for the realization of compact RFEH circuits that contribute immensely to mm-wave rectenna design. Effective mechanisms for configuring the rectenna modules based on the recommended spectrums for the RFEH system were also outlined. This study featured a conceptual viewpoint on design tradeoffs, which were accompanied by profound EH solutions perspectives for wireless power communications. The work covers some challenges attributed to 5G EH in mm-wave rectenna: from a controlled source of communication signals to distributed ambient EH and system level design. Conversely, the primary targets of this work are to: (I) examine a wide range of ambient RF sources and their performance with various antennae and RF-rectifier layouts; (II) propose unique rectenna design techniques suitable for current trends in wireless technology; (III) explore numerous approaches for enhancing the rectenna or RF-rectifier efficiency in a low-power ambient environment; and (IV) present the findings of a comprehensive review of the exemplary research that has been investigated. These are aimed toward addressing the autonomous system’s energy challenges. Therefore, with the careful management of the reported designs, the rectenna systems described in this study would influence the upcoming advancement of the low-power RFEH module.
CMOS Interface Circuits for High-Voltage Automotive Signals
Authors: Boni Andrea , Caselli Michele , Magnanini Alessandro , Tonelli Matteo
Published: March 2022

The acquisition of high-voltage signals from sensors and actuators in an internal-combustion engine is often required for diagnostic purposes or in the case of conversion to alternative fuels, such as hydrogen, natural gas, or biogas. The integration of electronic interfaces and acquisition circuits in a single device provides benefits in terms of component-count reduction and performance. Nonetheless, the high voltage level of the involved signals makes on-chip design challenging. Additionally, the circuits should be compatible with the CMOS technology, with limited use of high-voltage options and a minimum number of off-chip components. This paper describes the design and the implementation in 350 nm CMOS technology of electronic interfaces and acquisition circuits for typical high-voltage signals of automotive context. In particular, a novel co-design of dedicated voltage clamps with electro-static discharge (ESD) protections is described. The proposed circuits require only a single off-chip resistor, and they are suitable for the acquisition of signals with peak voltages up to 400 V. The measured performance of the silicon prototypes, in the [−40 °C, +125 °C] temperature range, make the proposed electronic interfaces suitable for the automotive domain.
Parameter Sensitivity Analysis and Robust Design Approach for Flux-Switching Permanent Magnet Machines
Authors: Zhang Gan , Tong Qing , Qiu Anjian , Xu Xiaohan , Hua Wei , Chen Zhihong
Published: March 2022

Parameter sensitivity analysis is usually required to select the key parameters with high sensitivity to the optimal goal before the optimization is carried out, especially for flux-switching permanent magnet (FSPM) machines where lot of design parameters should be considered. Unlike the traditional studies on parameter sensitivity, which are generally experience- or statistics-based, and are time-consuming, this paper proposes a parameter sensitivity analysis method of a FSPM machine based on a magnetic equivalent circuit (MEC), which enables the parameters’ sensitivities to be evaluated by their exponential in the nondimensionalized equations, thus providing a fast and accurate way to obtain the parameter sensitivities. Thereafter, the influences of modular manufacturing methods on magnetic performances are discussed, and the robust design approach for the FSPM machine is introduced, which aims to achieve the best machine stability and robustness by setting boundaries on design dimensions when taking into account the manufacturing tolerances. Experimental validations are also presented.
A Working Definition of Fake News
Authors: Baptista João , Gradim Anabela
Published: March 2022

Current literature on fake news is rather abundant and mainly focused on history, variety, and types, rather than processes. This review draws on current literature to build a working definition of fake news focused on its present relevance to journalism and political communication contemporary debate, distinguishing it from non-pertinent conceptual varieties and contributing to a much-needed clarification on the subject. We performed a qualitative analysis of the literature published between 2016 and 2020. Data were extracted from Web of Science and Scopus. We define fake news as a type of online disinformation with misleading and/or false statements that may or may not be associated with real events, intentionally designed to mislead and/or manipulate a specific or imagined public through the appearance of a news format with an opportunistic structure (title, image, content) to attract the reader’s attention in order to obtain more clicks and shares and, therefore, greater advertising revenue and/or ideological gain.
Alternatives to Enhance the Structural Performance of PET-Modified Reinforced Concrete Beams
Authors: Assaad Joseph J. , Khalil Mario , Khatib Jamal
Published: March 2022

Numerous studies investigated the possibility of incorporating plastic wastes in concrete mixtures, thus contributing to efficient management and sustainable development of the construction industry. This paper investigates the possibility of reducing the water-to-cement ratio and/or incorporating steel fibers or polymeric latexes to mitigate the drop in structural properties of reinforced concrete (RC) beams containing polyethylene terephthalate (PET) additions. The PET was derived from waste plastic bottles that were shredded into small pieces and added during concrete batching at 1.5% to 4.5%, by total volume. Test results showed that the concrete plain and structural properties degrade with PET additions, given their lightweight nature and poor characteristic strength compared to aggregate particles. The reduction of w/c from 0.55 to 0.46 proved efficient to refine the matrix porosity and reinstate the shear and flexural strengths of RC beams. Moreso, the incorporation of 0.8% steel fibers (by volume) or 15% styrene-butadiene rubber latexes (by mixing water) were efficient to enhance the bridging phenomenon and reduce the propagation of cracks during beam testing.
SWI/SNF Chromatin Remodeling Enzymes in Melanoma
Authors: Dreier Megan R. , de la Serna Ivana L.
Published: March 2022

Melanoma is an aggressive malignancy that arises from the transformation of melanocytes on the skin, mucosal membranes, and uvea of the eye. SWI/SNF chromatin remodeling enzymes are multi-subunit complexes that play important roles in the development of the melanocyte lineage and in the response to ultraviolet radiation, a key environmental risk factor for developing cutaneous melanoma. Exome sequencing has revealed frequent loss of function mutations in genes encoding SWI/SNF subunits in melanoma. However, some SWI/SNF subunits have also been demonstrated to have pro-tumorigenic roles in melanoma and to affect sensitivity to therapeutics. This review summarizes studies that have implicated SWI/SNF components in melanomagenesis and have evaluated how SWI/SNF subunits modulate the response to current therapeutics.