bluuebunny/crossref_metadata_2025 huggingface dataset:
An Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosensors—Application to Cancer Antigen 15-3
Authors: dos Santos Oliveira Daniela , Oliveira Andreia Sofia Rodrigues , Mendonça Patrícia Vitorino , Coelho Jorge Fernando Jordão , Moreira Felismina Teixeira Coelho , Sales Maria Goreti Ferreira
Published: April 2024

This work presents a novel approach for tailoring molecularly imprinted polymers (MIPs) with a preliminary stage of atom transfer radical polymerization (ATRP), for a more precise definition of the imprinted cavity. A well-defined copolymer of acrylamide and N,N′-methylenebisacrylamide (PAAm-co-PMBAm) was synthesized by ATRP and applied to gold electrodes with the template, followed by a crosslinking reaction. The template was removed from the polymer matrix by enzymatic/chemical action. The surface modifications were monitored via electrochemical impedance spectroscopy (EIS), having the MIP polymer as a non-conducting film designed with affinity sites for CA15-3. The resulting biosensor exhibited a linear response to CA15-3 log concentrations from 0.001 to 100 U/mL in PBS or in diluted fetal bovine serum (1000×) in PBS. Compared to the polyacrylamide (PAAm) MIP from conventional free-radical polymerization, the ATRP-based MIP extended the biosensor’s dynamic linear range 10-fold, improving low concentration detection, and enhanced the signal reproducibility across units. The biosensor demonstrated good sensitivity and selectivity. Overall, the work described confirmed that the process of radical polymerization to build an MIP material influences the detection capacity for the target substance and the reproducibility among different biosensor units. Extending this approach to other cancer biomarkers, the methodology presented could open doors to a new generation of MIP-based biosensors for point-of-care disease diagnosis.
“Take It One Dilation at a Time”: Caregiver Perspectives of Postoperative Anal Dilations in Pediatric Patients with Colorectal Conditions
Authors: Wehrli Lea A. , Ariefdjohan Merlin , Ketzer Jill , Matkins Kristina , De la Torre Luis , Bischoff Andrea , Judd-Glossy Laura
Published: April 2024

Background: Postoperative anal dilations (PAD) are the standard of care for patients after a posterior sagittal anorectoplasty (PSARP) for anorectal malformation (ARM) or a transanal pull-through (TP) procedure for Hirschsprung disease (HD). This study assessed the psychosocial impact of PAD among caregivers of children with ARM or HD, which may inform postoperative care strategies. Methods: Caregivers of patients with ARM and HD who underwent PSARP or TP within five years participated in the online survey. Questions included demographics, patient and caregiver experiences with PAD, and baseline psychosocial functioning. Quantitative results were reported descriptively, while qualitative responses were summarized as major themes. Results: The survey indicated a response rate of 26% caregivers, with most being female (91%) and biological mothers (85%). Patients were mostly male (65%), born with ARM (74%), and were five months old on average when PAD began. Caregivers reported that during PAD, children experienced distress (56%), pain (44%), and fear (41%), while a third noted no negative reactions. Over time, their child’s ability to cope with PAD got easier (38%) or stayed the same (41%). Caregivers reported worry/anxiety (88%), guilt (71%), stress (62%), and frustration (35%), noting that additional coping strategies to manage the emotional and logistical challenges of daily PAD would be helpful. Conclusion: Although PAD is necessary, it can be highly stressful for the patients and their caregivers. Key findings emphasized the need for additional coping strategies and highlighted the importance of integrating psychosocial support into the postoperative care regimen.
Construction of a Predictive Model for Dynamic and Static Recrystallization Kinetics of Cast TC21 Titanium Alloy
Authors: Li Ziliang , Chai Yunpeng , Qin Ling , Zhu Yanchun , Niu Yong , Fan Jiaxin , Yue Zhenwei
Published: April 2024

In this study, hot compression experiments were conducted on cast TC21 titanium alloy using a Gleeble-1500D thermal simulation compression tester, and the hot-compressed specimens were heat-treated. The data obtained after analyzing the thermal compression of cast TC21 titanium alloy were analyzed to construct a thermal machining diagram with a strain of 0.8 and to optimize the machining window. This study investigated the microstructure of the alloy after hot pressing experiments and heat treatment, applying the study of the microstructure evolution law of cast TC21 titanium alloy. The analysis of the tissue evolution law established the dynamic and static recrystallization volume fraction as a function of heat deformation parameters. The results show that the optimal processing window for cast TC21 titanium alloy is a deformation temperature in the range of 1373 K–1423 K and a strain rate of 0.1 s−1. The increase in deformation volume and deformation temperature both favor recrystallization and make the recrystallization volume fraction increase, but the increase in strain rate will inhibit the increase in the recrystallization degree to some extent. The dynamic and static recrystallization equations for the cast TC21 titanium alloy at different temperatures were constructed. The experimental measurements of recrystallization volume fraction are in good agreement with the predicted values.
Virtual Photon-Mediated Quantum State Transfer and Remote Entanglement between Spin Qubits in Quantum Dots Using Superadiabatic Pulses
Authors: Wang Yue , Wang Ting , Zhu Xing-Yu
Published: April 2024

Spin qubits in semiconductor quantum dots are an attractive candidate for scalable quantum information processing. Reliable quantum state transfer and entanglement between spatially separated spin qubits is a highly desirable but challenging goal. Here, we propose a fast and high-fidelity quantum state transfer scheme for two spin qubits mediated by virtual microwave photons. Our general strategy involves using a superadiabatic pulse to eliminate non-adiabatic transitions, without the need for increased control complexity. We show that arbitrary quantum state transfer can be achieved with a fidelity of 95.1% within a 60 ns short time under realistic parameter conditions. We also demonstrate the robustness of this scheme to experimental imperfections and environmental noises. Furthermore, this scheme can be directly applied to the generation of a remote Bell entangled state with a fidelity as high as 97.6%. These results pave the way for fault-tolerant quantum computation on spin quantum network architecture platforms.
Experiments on the Efficacy of Drawing for Memorization among Adults and Children with Varying Written Word Memory Capacities: A Two-Way Crossover Design
Authors: Óttarsdóttir Unnur Guðrún
Published: April 2024

In recent years, the comparative effectiveness of drawing and writing for memory has been investigated, but the findings have mostly been analyzed for the entire sample of participants rather than subgroups. In quantitative two-way crossover experiments involving 134 children and 262 adults, drawing for memorization as compared to writing was investigated. The participants were divided into subgroups based on their ability to recall the greatest, moderate, or fewest words and drawings. The difference in the number of recalled words and drawings was then compared between subgroups with varying memory capacities for written words and drawings. Participants who had difficulty remembering written words recalled more drawings than written words relative to participants who remembered written words more easily—this applied to shorter- and longer-term memory. To determine the applicability of the findings to various contexts, the following conditions were varied in four separate experiments: participant age, duration of encoding and recall, number of words memorized, interval between encoding and recall, and the research setting. Drawing benefited memory more than writing in all tested scenarios for the subgroup that remembered the fewest number of words. The new finding of the study is that people who have difficulty remembering written words benefit the most from drawing for memorization compared to those who remember written words more easily and this applies to the various tested conditions.
P2P Energy Trading of EVs Using Blockchain Technology in Centralized and Decentralized Networks: A Review
Authors: Khan Sara , Amin Uzma , Abu-Siada Ahmed
Published: April 2024

Peer-to-peer (P2P) energy trading has attracted a lot of attention and the number of electric vehicles (EVs) has increased in the past couple of years. Toward sustainable mobility, EVs meet the standard development goals (SDGs) for attaining a sustainable future in the transport sector. This development and increasing number of EVs creates an opportunity for prosumers to trade electricity. Considering this opportunity, this review article aims to provide an in-depth analysis of P2P energy trading of EVs using blockchain in centralized and decentralized networks, which enables prosumers to exchange energy directly with one another. The paper is aimed to provide the reader with a state-of-the-art review on the P2P energy trading for EVs, considering different blockchain algorithms that are practically implemented or still in the research phase. Moreover, the paper presents blockchain applications, current trends, and future challenges of EVs’ energy trading. P2P energy trading for EVs using blockchain algorithms can be successfully implemented considering real-time scenarios and economically benefits smart sustainable societies.
Techno-Economic Evaluation on Solar-Assisted Post-Combustion CO2 Capture in Hollow Fiber Membrane Contactors
Authors: Mu Junkun , Bi Jinpeng , Lv Yuexia , Su Yancai , Zhao Wei , Zhang Hui , Du Tingting , Li Fuzhao , Zhou Hongyang
Published: April 2024

In this study, a novel system which integrates solar thermal energy with membrane gas absorption technology is proposed to capture CO2 from a 580 MWe pulverized coal power plant. Technical feasibility and economic evaluation are carried out on the proposed system in three cities with different solar resources in China. Research results show that the output capacity and net efficiency of the SOL-HFMC power plant are significantly higher than those of the reference power plant regardless of whether a TES system is applied or not. In addition, the CEI of the SOL-HFMC power plant with the TES system is 4.36 kg CO2/MWh, 4.45 kg CO2/MWh and 4.66 kg CO2/MWh lower than that of the reference power plant. The prices of the membrane, vacuum tube collector and phase change material should be reduced to achieve lower LCOE and COR values. Specifically for the SOL-HFMC power plant with the TES system, the corresponding vacuum tube collector price shall be lower than 25.70 $/m2 for Jinan, 95.20 $/m2 for Xining, and 128.70 $/m2 for Lhasa, respectively. To be more competitive than a solar-assisted ammonia-based post-combustion CO2 capture power plant, the membrane price in Jinan, Xining and Lhasa shall be reduced to 0.012 $/m, 0.015 $/m and 0.016 $/m for the sake of LCOE, and 0.03 $/m, 0.033 $/m and 0.034 $/m for the sake of COR, respectively.
Study of Phase Changes in Operational Risk for Trucks
Authors: Niewczas Andrzej , Andrzejczak Karol , Mórawski Łukasz , Dębicka Ewa
Published: April 2024

This study concerns the management of operational risk in truck transport using the reliability theory of risks. In this regard, the risk analysis of changes in the vehicle unavailability represents an important topic. In this study, the authors present their own method for analysing the phase changes in risk corresponding to successive sections (phases) of vehicle mileage. The presented risk analysis method is based on an integrated assessment of losses associated with the costs of incidental repairs and losses caused by lost income during vehicle downtime. This includes the following: assessment of differences in average risk and differences in the coefficient of variation in the time series of vehicle mileage phases, indicating the outliers and extremes of phase risk, identifying their physical causes and testing the statistical significance of phase risk differences. The proposed method is described mathematically and verified experimentally based on the operational data concerning trucks from two selected brands (20 trucks from each brand). We show that the method can be used to predict the continuity of transport services in the short term (one-year horizon). The method could also be useful to compare vehicles of different brands in the context of their sensitivity to operational risks.
The COVID-19 Pandemic Was Associated with a Change in Therapeutic Management and Mortality in Heart Failure Patients
Authors: Ponzoni Matteo , Morabito Gabriella , Corrao Giovanni , Gerosa Gino , Cantarutti Anna , Rea Federico
Published: April 2024

Background: Heart failure (HF)-related mortality has been exacerbated by the COVID-19 pandemic; however, it is unclear how healthcare reassessment has contributed to the excess mortality versus SARS-CoV-2 infection itself. We aimed to assess how the pandemic affected the therapeutic management and prognosis of HF patients. Methods: We retrospectively reviewed the healthcare utilization databases of the Lombardy region (Italy) to identify all newly-diagnosed HF patients from January 2018 to December 2021. Outcomes were the utilization of HF therapies (Sacubitril/Valsartan; cardiac resynchronization therapy [CRT] and/or implantable cardioverter-defibrillator [ICD]; mechanical circulatory support [MCS]; heart transplantation [HTX]) and mortality. Cox regression models were fitted to estimate the hazard ratios (HR) and 95% confidence intervals (CI) for outcomes associated with the pandemic. Results: 36,130 and 17,263 patients were identified in the pre-pandemic and pandemic eras, respectively. The pandemic reduced Sacubitril/Valsartan utilization (HR = 0.77, 95% CI: 0.65–0.91) and CRT/ICD implantation (HR = 0.85, 95% CI: 0.78–0.92), but not MCS (HR = 1.11, 95% CI: 0.86–1.43) and HTX (HR = 0.88, 95% CI: 0.70–1.09). An increased mortality risk was observed during the pandemic (HR = 1.19, 95% CI: 1.15–1.23), which was attributable to SARS-CoV-2 infection (HR for non-COVID-19-related mortality = 1.01, 95% CI: 0.97–1.04). Conclusions: The COVID-19 pandemic was associated with a reduction in medical and interventional therapies for HF and an increase in mortality for HF patients.
The Interplay of Sleep Quality, Mental Health, and Sociodemographic and Clinical Factors among Italian College Freshmen
Authors: Dagani Jessica , Buizza Chiara , Cela Herald , Sbravati Giulio , Rainieri Giuseppe , Ghilardi Alberto
Published: April 2024

Background/Objectives: Sleep and mental health are closely linked, with sleep deprivation increasing the risk of mental health problems in college students. This study aimed to analyze the role of sleep in the mental health status of a sample of Italian freshmen, considering various mental health outcomes and potential interactions between sleep and other relevant factors, such as sociodemographic characteristics, academic experiences, and mental health history. Methods: All freshmen from a medium-sized Italian university were invited to participate in a multidimensional online survey (n = 3756). Sleep quality was assessed through questions on average hours of sleep per night and on satisfaction of perceived sleep quality. Mental health outcomes included psychophysical well-being, psychological distress, substance use, and problematic internet use. Statistical analysis involved multivariate analysis of variance, followed by pairwise comparisons. Results: The sample (n = 721) exhibited low levels of well-being and a high prevalence of psychological distress (52.1%). Approximately one-third of students (n = 258) were dissatisfied with their sleep quality, and one-fourth (n = 186) reported inadequate sleep (less than 7 h per night). More specifically, 24.4% of students slept on average six hours per night, and 1.4% slept five hours or less. Satisfaction with perceived sleep quality significantly influenced well-being, psychological distress, and cannabis use (ηp2 = 0.02). Interaction effects were observed between satisfaction with sleep quality and drop-out intentions (ηp2 = 0.01), as well as between satisfaction with sleep quality and history of mental health diagnosis (ηp2 = 0.02), both of which were significant for psychological distress and cannabis use. Conclusions: This study highlights the influence of perceived sleep quality on academic distress among college freshmen, particularly those with higher intentions of leaving university and with a history of mental health diagnosis.
Periprosthetic Hip Fractures around the Stem: Can the Stem Design Affect Fracture Features?
Authors: Comba Luca Costanzo , Gagliardi Luca , Onorato Francesco , Rivera Fabrizio
Published: April 2024

Background: Total hip arthroplasty is one of the most successful orthopedic surgeries; nevertheless, many of these surgeries are the causes of failure, and among them, periprosthetic fractures are one of the major causes of revision. Our study focuses on periprosthetic hip fractures with two different stem designs. The aim of the study was to analyze the obtained results, focusing on the features of periprosthetic stem fractures observed. Methods: We retrospectively reviewed periprosthetic fractures occurring between 2010 and 2023, involving Alloclassic® or CLS® uncemented femoral stems. We analyzed demographic data, proximal femur morphology, and the fracture type. Results: We identified 97 patients. Considering the proximal femur morphology, we found that there was statistically significant prevalence of Dorr A proximal femur morphology in the CLS® group and of Dorr C in the Alloclassic® group. Considering the distribution of the fracture pattern, we reported a non-statistically significant prevalence of the fracture pattern with stable stems in the CLS® group. Conclusions: The choice of the prosthetic design of the femoral stem is a crucial element when planning total hip arthroplasty. However, we found a non-statistically significant difference between the two stems considered, raising questions about the real role of stem design as a primary determinant of periprosthetic hip fractures.
Uterine Balloon Tamponade under Ultrasound Guidance in Women with Postpartum Hemorrhage: A Retrospective Cohort Study
Authors: Germano Chiara , Girlando Flavia , Carosso Andrea Roberto , Messina Alessandro , Parpinel Giulia , Leo Livio , Attini Rossella , Revelli Alberto , Masturzo Bianca
Published: April 2024

Background: Postpartum hemorrhage (PPH) represents one of the principal causes of maternal mortality and morbidity worldwide. Uterine balloon tamponade (UBT) is recommended for the treatment of postpartum hemorrhage due to uterine atony in women who do not respond to pharmacological first-line treatment. The success of UBT in bleeding control is related to the correct positioning of the device, since incorrect insertion can be associated with ineffectiveness and requires time-consuming repositioning maneuvers, with a further increase in blood loss. The use of ultrasound (US) during UBT may increase the success rate by preventing wrong positioning. This study aims to demonstrate the role of US guidance during UBT and to assess whether US guidance can ease positioning and reduce insertion times, preventing additional repositioning maneuvers, in comparison with a US check carried out after balloon insertion. Methods: This was a retrospective study including 370 women who underwent vaginal delivery, had PPH caused by uterine atony and required UBT at Sant’Anna Hospital from 2015 to 2019. The exclusion criteria were an abnormal placental site, vaginal or cervical trauma, coagulation defects, uterine anomalies, previous postpartum hemorrhage and previous caesarean section. Included subjects were divided into two groups: the US-guided group (n = 200) underwent Bakri balloon positioning under US guidance, and the non-guided group (n = 170) received a US check only after balloon insertion. The primary outcome was the success rate of the procedure, expressed as the percentage of cases with bleeding control without the need for balloon repositioning; the secondary outcomes were the length of the procedure and some parameters related to blood loss. Results: The success rate was 99% for the US-guided group vs. 86% for the non-guided group. None of the patients required hysterectomy. In the US-guided group with respect to the non-guided group, we observed a significant reduction in blood loss (1100 ± 450 vs. 1500 ± 600 mL; p < 0.001), Δhemoglobin level (1.8 ± 1.1 vs. 2.7 ± 2.0 g/dL, p < 0.001) and time required for the procedure (8 vs. 13 min, p < 0.001). Conclusions: Our data suggest that the use of US guidance for placement of UBT was associated with reduced need for balloon repositioning, lower blood loss, and faster resolution of postpartum hemorrhage.
A Review of the Current Approach and Treatment Landscape for Stage III Non-Small Cell Lung Cancer
Authors: Sridhar Arthi , Khan Hina , Yohannan Binoy , Chan Kok Hoe , Kataria Nilansh , Jafri Syed Hasan
Published: April 2024

The therapeutic landscape of the management of stage III non-small cell lung cancer (NSCLC) has drastically evolved with the incorporation of immunotherapy and targeted therapy. Stage III NSCLC accounts for one-third of the cases and the treatment strategy of these locally advanced presentations are diverse, ranging from surgical to non-surgical options; with the incorporation of chemo-immunotherapy, radiation, and targeted therapies wherever applicable. The staging of this disease has also changed, and it is essential to have a strong multidisciplinary approach to do justice to patient care. In this article, we aim to navigate the nuanced approaches in the diagnosis and treatment of stage III NSCLC and expand on the evolution of the management of this disease.
Characterization of Perioperative Serotonin in Patients Undergoing Orthotopic Liver Transplantation
Authors: Zott Tobias , Pereyra David , Kersten Isabelle , Ortner Max , Hüpper Maria Noelle , Starlinger Patrick , Berlakovich Gabriela A. , Silberhumer Gerd R.
Published: April 2024

Background: Platelets were shown to be relevant for liver regeneration. In particular, platelet-stored serotonin (5-HT) proved to be a pro-regenerative factor in this process. The present study aimed to investigate the perioperative course of 5-HT and evaluate associations with patient and graft outcomes after othotopic liver transplantation (OLT). Methods: 5-HT was quantified in plasma and serum of 44 OLT recipients perioperatively, and in their respective donors. Olthoff’s criteria for early allograft dysfunction (EAD) were used to evaluate postoperative outcomes. Results: Patients with higher donor intra-platelet 5-HT per platelet (IP 5-HT PP) values had significantly lower postoperative transaminases (ASAT POD1: p = 0.006, ASAT POD5: p = 0.006, ASAT POD10: p = 0.02, ALAT POD1: p = 0.034, ALAT POD5: p = 0.017, ALAT POD10: p = 0.04). No significant differences were seen between postoperative 5-HT values and the occurrence of EAD. A tendency was measured that donor IP 5-HT PP is lower in donor-recipient pairs that developed EAD (p = 0.07). Conclusions: Donor IP 5-HT PP might be linked to the postoperative development of EAD after OLT, as higher donor levels are correlated with a more favorable postoperative course of transaminases. Further studies with larger cohorts are needed to validate these findings.
Evaluation of Target Attainment for Tobramycin in Children and Adults with Cystic Fibrosis
Authors: Struiken Sheseira T. L. , Lobée Danique , Tuinen Eline L. van , Touw Daniel J. , Vaart Hester van der , Bourgonje Arno R. , Rottier Bart L. , Koppelman Gerard H. , Mian Paola
Published: April 2024

Introduction: Patients with cystic fibrosis (CF) commonly experience pulmonary exacerbations, and it is recommended by the TOPIC study to treat this with tobramycin at a dose of 10 mg/kg once daily. The aim of this study was to evaluate the target attainment of the current dosing regimen. Methods: A single-center retrospective cohort study of child and adult patients with CF who received tobramycin between 2019 and 2022 was conducted. Descriptive statistics and linear mixed models were used to assess target attainment for tobramycin. Results: In total, 25 patients (53 courses), of which 10 were children (12 courses) and 15 were adults (41 courses), were included. Those 25 patients all received 10 mg/kg/day. The tobramycin peak concentrations were supratherapeutic in 82.9% and therapeutic in 100.0% of adults and children, respectively. The trough concentrations were outside the target range in 0% and 5.1% of children and adults, respectively. We found lower tobramycin concentrations with the same dose in children compared to adults. Conclusions: This study illustrates the need to validate dosing advice in a real-world setting, as supratherapeutic concentrations of tobramycin were prevalent in adults with CF.
Percutaneous Mechanical Circulatory Support in Acute Heart Failure Complicated with Cardiogenic Shock
Authors: Xenitopoulou Maria , Ziampa Kyriaki , Evangeliou Alexandros , Tzikas Stergios , Vassilikos Vasileios
Published: April 2024

Despite advancements in algorithms concerning the management of cardiogenic shock, current guidelines still lack the adequate integration of mechanical circulatory support devices. In recent years, more and more devices have been developed to provide circulatory with or without respiratory support, when conservative treatment with inotropic agents and vasopressors has failed. Mechanical circulatory support can be contemplated for patients with severe, refractory, or acute-coronary-syndrome-related cardiogenic shock. Through this narrative review, we delve into the differences among the types of currently used devices by presenting their notable advantages and inconveniences. We address the technical issues emerging while choosing the best possible device, temporarily as a bridge to another treatment plan or as a destination therapy, in the optimal timing for each type of patient. We also highlight the diverse implantation and removal techniques to avoid major complications such as bleeding and limb ischemia. Ultimately, we hope to shed some light in the gaps of evidence and the importance of conducting further organized studies around the topic of mechanical circulatory support when dealing with such a high mortality rate.
Waves of Precision: A Practical Guide for Reviewing New Tools to Evaluate Mechanical In-Exsufflation Efficacy in Neuromuscular Disorders
Authors: Chatwin Michelle , Sancho Jesus , Lujan Manel , Andersen Tiina , Winck Joao-Carlos
Published: April 2024

Mechanical insufflation-exsufflation (MI-E) is essential for secretion clearance, especially in neuromuscular disorders. For the best outcomes, initiation of MI-E should be started at the correct time with regular evaluation to the response to treatment. Typically, cough peak flow has been used to evaluate cough effectiveness with and without MI-E. This review highlights the limitations of this and discussed other tools to evaluate MI-E efficacy in this rapidly developing field. Such tools include the interpretation of parameters (like pressure, flow and volumes) that derive from the MI-E device and external methods to evaluate upper airway closure. In this review we pinpoint the differences between different devices in the market and discuss new tools to better titrate MI-E and detect pathological responses of the upper airway. We discuss the importance of point of care ultrasound (POCUS), transnasal fiberoptic laryngoscopy and wave form analysis in this setting. To improve clinical practice newer generation MI-E devices should allow real-time evaluation of waveforms and standardize some of the derived parameters.
Major Orthopaedic Surgery in Persons with Haemophilia A with and without Inhibitors Treated by Emicizumab: A Mid-Term, Large, and Successful Series at a Single Center
Authors: Carulli Christian , Daniele Giovanna , Linari Silvia , Pieri Lisa , Littera Mariastefania , Mazzetti Matteo , Tamburini Carlo , Prisco Domenico , Castaman Giancarlo
Published: April 2024

Introduction: Patients with Haemophilia (PWH) need orthopaedic treatments and often they undergo surgery. Classically, PWH with inhibitors have to face such procedures earlier than other patients. Major orthopaedic surgery is not easy and complications are frequent. Emicizumab is the first monoclonal antibody introduced for haematological prophylaxis for PWH with inhibitors, achieving an efficacious haemostasis also in patients with severe haemophilia A with inhibitors, later demonstrated for PWH without inhibitors. A few years ago, emicizumab was also proposed for PWH undergoing surgery, as it supports excellent bleeding control. The literature on orthopaedic surgery using an emicizumab protocol is scarce: only isolated case reports with short-term follow-ups are available. Aim: The purpose of this study is the assessment of the mid-term outcomes of major orthopaedic surgery performed in a population of patients with and without inhibitors and an emicizumab regimen. Methods: We reviewed the records of 13 PWH (eight with high-titre inhibitors, five without) with a mean age of 54.6 years, undergoing 15 orthopaedic surgical procedures between 2017 and 2022: primary knee and hip arthroplasty, revision, pseudotumor excision, or amputation. Their prophylaxis consisted of the combination of emicizumab and boluses of rFVIIa (PWH with inhibitors) or rFVIII (PWH without inhibitors). The clinical parameters of evaluation were: VAS, Haemophilic Joint Health Score (HJHS), and standard radiologic studies. Follow-up was conducted at 1, 3, 6 months, and then yearly. The survival rate of all implants was also assessed. Results: The mean follow-up was 38.8 months (range: 12–65). All patients were successfully treated without complications during surgery. During the postoperative period, a patient affected by a septic complication two months after his pseudotumor excision underwent an above-the-knee amputation. All patients were regularly discharged to the rehabilitative ward, reporting satisfaction for pain reduction and improved joint and global function at the VAS and HJHS scores. No revisions or implant failures were recorded. Conclusions: A prophylaxis regimen with emicizumab and factor replacement in PWH with or without inhibitors undergoing major orthopaedic surgery ensures effective bleeding control and good postoperative clinical outcomes at mid-term follow-up, and may be routinely adopted in dedicated high-volume hospitals. This series is the most consistent to date reported at a single Haemophilia centre.
The Effect of Open and Closed Oocyte Vitrification Systems on Embryo Development: A Systematic Review and Network Meta-Analysis
Authors: Pantos Konstantinos , Maziotis Evangelos , Trypidi Anna , Grigoriadis Sokratis , Agapitou Kristi , Pantou Agni , Nikolettos Konstantinos , Kokkini Georgia , Sfakianoudis Konstantinos , Pomeroy Kimball O. , Simopoulou Mara
Published: April 2024

Background/Objectives: Open and closed vitrification systems are commonly employed in oocyte cryopreservation; however, there is limited evidence regarding a comparison of their separate impact on oocyte competence. This study uniquely brings to the literature, data on the effect of open versus closed vitrification systems on laboratory and clinical outcomes, and the effect of cooling and warming rates. Methods: A systematic search of the literature was performed using the databases PubMed/MEDLINE and the Cochrane Central Library, limited to articles published in English up to January 2023. A network meta-analysis was conducted comparing each vitrification system versus fresh oocytes. Results: Twenty-three studies were included. When compared to fresh oocytes, both vitrification devices resulted in lower fertilization rates per MII oocyte retrieved. When comparing the two systems in terms of survival rates, no statistically significant difference was observed. However, interestingly open systems resulted in lower cleavage and blastocyst formation rates per 2 pronuclear (2PN) oocyte compared to fresh controls, while at the same time no statistically significant difference was detected when comparing closed devices with fresh oocytes. Conclusions: In conclusion, closed vitrification systems appear to exert a less detrimental impact on the oocytes’ competence, which is reflected in the blastocyst formation rates. Proof of superiority of one system versus the other may lead to standardization, helping to ultimately determine optimal practice in oocyte vitrification.
Laparoscopic Management of Cesarean Scar Pregnancy with Temporary Clipping of Anterior Trunk of Hypogastric Arteries: A Case Report
Authors: Bacila Ioana-Flavia , Balulescu Ligia , Dabica Alexandru , Brasoveanu Simona , Pirtea Marilena , Ratiu Adrian , Pirtea Laurentiu
Published: April 2024

A cesarean scar ectopic pregnancy (CSP) represents an uncommon yet potentially life-threatening condition requiring immediate and efficient management. We present a case of a 32-year-old woman diagnosed with a scar pregnancy at 8 weeks of gestation. Laparoscopic surgical management was chosen due to its minimally invasive nature and potential for preserving fertility. During the procedure, temporary clipping of uterine arteries was employed to control intraoperative bleeding. The patient recovered well postoperatively with no complications. This case highlights the feasibility and effectiveness of laparoscopic intervention combined with temporary uterine artery clipping in the management of scar pregnancies, offering a valuable approach for clinicians faced with similar cases. Through this report, we aim to contribute to the existing literature on the optimal management of CSP and highlight the efficacy of laparoscopic surgery in this context.
A Multi-Agent Reinforcement Learning-Based Grant-Free Random Access Protocol for mMTC Massive MIMO Networks
Authors: Bueno Felipe Augusto Dutra , Goedtel Alessandro , Abrão Taufik , Marinello José Carlos
Published: April 2024

The expected huge number of connected devices in Internet of Things (IoT) applications characterizes the massive machine-type communication (mMTC) scenario, one prominent use case of beyond fifth-generation (B5G) systems. To meet mMTC connectivity requirements, grant-free (GF) random access (RA) protocols are seen as a promising solution due to the small amount of data that MTC devices usually transmit. In this paper, we propose a GF RA protocol based on a multi-agent reinforcement learning approach, applied to aid IoT devices in selecting the least congested RA pilots. The rewards obtained by the devices in collision cases resemble the congestion level of the chosen pilot. To enable the operation of the proposed method in a realistic B5G network scenario and aiming to reduce signaling overheads and centralized processing, the rewards in our proposed method are computed by the devices taking advantage of a large number of base station antennas. Numerical results demonstrate the superior performance of the proposed method in terms of latency, network throughput, and per-device throughput compared with other protocols.
Machine Learning-Based Simulation of the Air Conditioner Operating Time in Concrete Structures with Bayesian Thresholding
Authors: Jang Changhwan , Kim Hong-Gi , Woo Byeong-Hun
Published: April 2024

Efficient energy use is crucial for achieving carbon neutrality and reduction. As part of these efforts, research is being carried out to apply a phase change material (PCM) to a concrete structure together with an aggregate. In this study, an energy consumption simulation was performed using data from concrete mock-up structures. To perform the simulation, the threshold investigation was performed through the Bayesian approach. Furthermore, the spiking part of the spiking neural network was modularized and integrated into a recurrent neural network (RNN) to find accurate energy consumption. From the training-test results of the trained neural network, it was possible to predict data with an R2 value of 0.95 or higher through data prediction with high accuracy for the RNN. In addition, the spiked parts were obtained; it was found that PCM-containing concrete could consume 32% less energy than normal concrete. This result suggests that the use of PCM can be a key to reducing the energy consumption of concrete structures. Furthermore, the approach of this study is considered to be easily applicable in energy-related institutions and the like for predicting energy consumption during the summer.
Thermo-Mechanical Optimization of Die Casting Molds Using Topology Optimization and Numerical Simulations
Authors: Djabraian Serouj , Teichmann Fabian , Müller Sebastian
Published: April 2024

Conventional cooling channels used in die casting molds exhibit significant drawbacks, resulting in extended cooling times for cast parts. Issues such as the formation of dirt, limescale, and corrosion substantially diminish the thermal efficiency of these channels, leading to challenges in achieving uniform cooling and potential quality issues. In response to these challenges, this study proposes Topology Optimization as a novel approach. It involves designing cooling structures through Topology Optimization to replace traditional cooling channels, incorporating both Discrete and Gaussian boundary conditions to optimize thermal efficiency. Additionally, Structural Topology Optimization is employed to ensure structural integrity, preventing deformation or yielding under high loads during the die casting process. Numerical analysis revealed superior thermal performance compared to conventional channels, particularly when subjected to Discrete and Gaussian boundary conditions. Furthermore, the application of the latter establishes conformal cooling and minimizes temperature gradients in the casting, reducing casting defects such as shrinkage porosity. These findings highlight the efficacy of Topology Optimization in addressing the challenges of traditional cooling methods, with wide-ranging implications for manufacturing processes utilizing permanent molds for shaping materials.
Theoretical Study of Cyanidin-Resveratrol Copigmentation by the Functional Density Theory
Authors: Chávez Breyson Yaranga , Paz José L. , Gonzalez-Paz Lenin A. , Alvarado Ysaias J. , Contreras Julio Santiago , Loroño-González Marcos A.
Published: April 2024

Anthocyanins are colored water-soluble plant pigments. Upon consumption, anthocyanins are quickly absorbed and can penetrate the blood–brain barrier (BBB). Research based on population studies suggests that including anthocyanin-rich sources in the diet lowers the risk of neurodegenerative diseases. The copigmentation caused by copigments is considered an effective way to stabilize anthocyanins against adverse environmental conditions. This is attributed to the covalent and noncovalent interactions between colored forms of anthocyanins (flavylium ions and quinoidal bases) and colorless or pale-yellow organic molecules (copigments). The present work carried out a theoretical study of the copigmentation process between cyanidin and resveratrol (CINRES). We used three levels of density functional theory: M06-2x/6-31g+(d,p) (d3bj); ωB97X-D/6-31+(d,p); APFD/6-31+(d,p), implemented in the Gaussian16W package. In a vacuum, the CINRES was found at a copigmentation distance of 3.54 Å between cyanidin and resveratrol. In water, a binding free energy ∆G was calculated, rendering −3.31, −1.68, and −6.91 kcal/mol, at M06-2x/6-31g+(d,p) (d3bj), ωB97X-D/6-31+(d,p), and APFD/6-31+(d,p) levels of theory, respectively. A time-dependent density functional theory (TD-DFT) was used to calculate the UV spectra of the complexes and then compared to its parent molecules, resulting in a lower energy gap at forming complexes. Excited states’ properties were analyzed with the ωB97X-D functional. Finally, Shannon aromaticity indices were calculated and isosurfaces of non-covalent interactions were evaluated.
Therapeutic Applications of Nanomedicine: Recent Developments and Future Perspectives
Authors: Rehan Farah , Zhang Mingjie , Fang Jun , Greish Khaled
Published: April 2024

The concept of nanomedicine has evolved significantly in recent decades, leveraging the unique phenomenon known as the enhanced permeability and retention (EPR) effect. This has facilitated major advancements in targeted drug delivery, imaging, and individualized therapy through the integration of nanotechnology principles into medicine. Numerous nanomedicines have been developed and applied for disease treatment, with a particular focus on cancer therapy. Recently, nanomedicine has been utilized in various advanced fields, including diagnosis, vaccines, immunotherapy, gene delivery, and tissue engineering. Multifunctional nanomedicines facilitate concurrent medication delivery, therapeutic monitoring, and imaging, allowing for immediate responses and personalized treatment plans. This review concerns the major advancement of nanomaterials and their potential applications in the biological and medical fields. Along with this, we also mention the various clinical translations of nanomedicine and the major challenges that nanomedicine is currently facing to overcome the clinical translation barrier.
Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages
Authors: Wen Ruiding , Luo Lu , Zhang Runcheng , Zhou Xudong , Wang Wei , Gong Limin
Published: April 2024

A neutral Polygonatum cyrtonema polysaccharide (NPCP) was isolated and purified from Polygonatum cyrtonema by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
Insights into Wheat Genotype‒Sphaerodes mycoparasitica Interaction to Improve Crop Yield and Defence against Fusarium graminearum: An Integration of FHB Biocontrol in Canadian Wheat Breeding Programmes
Authors: Powell Antonia , Kim Seon Hwa , Hucl Pierre , Vujanovic Vladimir
Published: April 2024

Fusarium head blight (FHB) is a major threat to wheat crop production and food security worldwide. The creation of resistant wheat cultivars is an essential component of an integrated strategy against Fusarium graminearum, the primary aetiological agent that causes FHB. The results of this study show that the deployment of proto-cooperative interactions between wheat genotypes and mycoparasitic biocontrol agents (BCAs) can improve crop yield and plant resistance in controlling the devastating effects of FHB on wheat agronomic traits. A Fusarium-specific mycoparasite, Sphaerodes mycoparasitica, was found to be compatible with common and durum wheat hosts, thus allowing the efficient control of F. graminearum infection in plants. Four genotypes of wheat, two common wheat, and two durum wheat cultivars with varying FHB resistance levels were used in this greenhouse study. The BCA treatments decreased FHB symptoms in all four cultivars and improved the agronomic traits such as spike number, spike weight, seed weight, plant biomass, and plant height which are vital to grain yield. Conversely, the F. graminearum 3ADON chemotype treatment decreased the agronomic trait values by up to 44% across cultivars. Spike number, spike weight, and seed weight were the most improved traits by the BCA. A more measurable improvement in agronomic traits was observed in durum wheat cultivars compared to common wheat.
Plant Cyanogenic-Derived Metabolites and Herbivore Counter-Defences
Authors: Martinez Manuel , Diaz Isabel
Published: April 2024

The release of cyanide from cyanogenic precursors is the central core of the plant defences based on the cyanogenesis process. Although cyanide is formed as a coproduct of some metabolic routes, its production is mostly due to the degradation of cyanohydrins originating from cyanogenic glycosides in cyanogenic plants and the 4-OH-ICN route in Brassicaceae. Cyanohydrins are then hydrolysed in a reversible reaction generating cyanide, being both, cyanohydrins and cyanide, toxic compounds with potential defensive properties against pests and pathogens. Based on the production of cyanogenic-derived molecules in response to the damage caused by herbivore infestation, in this review, we compile the actual knowledge of plant cyanogenic events in the plant–pest context. Besides the defensive potential, the mode of action, and the targets of the cyanogenic compounds to combat phytophagous insects and acari, special attention has been paid to arthropod responses and the strategies to overcome the impact of cyanogenesis. Physiological and behavioural adaptations, as well as cyanide detoxification by β-cyanoalanine synthases, rhodaneses, and cyanases are common ways of phytophagous arthropods defences against the cyanide produced by plants. Much experimental work is needed to further understand the complexities and specificities of the defence–counter-defence system to be applied in breeding programs.
Adaptive Fabrication of Electrochemical Chips with a Paste-Dispensing 3D Printer
Authors: Wong Ten It , Ng Candy , Lin Shengxuan , Chen Zhong , Zhou Xiaodong
Published: April 2024

Electrochemical (EC) detection is a powerful tool supporting simple, low-cost, and rapid analysis. Although screen printing is commonly used to mass fabricate disposable EC chips, its mask is relatively expensive. In this research, we demonstrated a method for fabricating three-electrode EC chips using 3D printing of relatively high-viscosity paste. The electrodes consisted of two layers, with carbon paste printed over silver/silver chloride paste, and the printed EC chips were baked at 70 °C for 1 h. Engineering challenges such as bulging of the tubing, clogging of the nozzle, dripping, and local accumulation of paste were solved by material selection for the tube and nozzle, and process optimization in 3D printing. The EC chips demonstrated good reversibility in redox reactions through cyclic voltammetry tests, and reliably detected heavy metal ions Pb(II) and Cd(II) in solutions using differential pulse anodic stripping voltammetry measurements. The results indicate that by optimizing the 3D printing of paste, EC chips can be obtained by maskless and flexible 3D printing techniques in lieu of screen printing.