bluuebunny/crossref_metadata_2025 huggingface dataset:
Socioeconomic and Environmental Aspects of Traditional Firewood for Cooking on the Example of Rural and Peri-Urban Mexican Households
Authors: Pérez Guadalupe , Islas-Samperio Jorge M. , Grande-Acosta Genice K. , Manzini Fabio
Published: July 2022

Firewood is a solid biofuel that is widely used for cooking in Mexico’s residential sector. This study seeks to identify relevant factors in firewood consumption patterns, and their implications for climate change, gender, and health, and for energy poverty in Mexico, by climate region and socioeconomic level. For this purpose, a statistical analysis was conducted of recently published official information. We estimate that a total of 31.3 million Mexicans—26% of the total population—use firewood, and we have identified three main types of users: (i) exclusive firewood users (30%); (ii) mixed firewood users using firewood as their primary fuel (18%) and (iii) mixed firewood users using firewood as their secondary fuel source (52%). Total consumption of firewood was estimated at 116.6 PJ, while estimated greenhouse gas emissions were 8.1 million tCO2e. Out of all the households studied, 53% were in the tropical climate region; 59% were categorized as being in the “low” socioeconomic level; and 75% were in population centers comprising fewer than 2500 inhabitants. Some 68% of households do not pay for the acquisition of firewood, and for those households that do pay for the resource, estimated transactions total USD 286.9 million. Expenditures on firewood for energy represent up to 10% of household income. Finally, it was estimated that 15.7 million direct users of firewood are women who use the resource in three-stone fires, in which they expose themselves to health risks in doing so. In conclusion, main universal findings, the study’s limitations, and future research are presented.
A Theoretical Evaluation of the Efficiencies of Metal-Free 1,3,4-Oxadiazole Dye-Sensitized Solar Cells: Insights from Electron–Hole Separation Distance Analysis
Authors: Coetzee Louis-Charl Cloete , Adeyinka Adedapo Sunday , Magwa Nomampondo
Published: July 2022

Herein, some novel metal-free 1,3,4-oxadiazole compounds O1–O7 were evaluated for their photovoltaic properties using density functional theory (DFT) and time-dependent density functional theory (TD-DFT) calculations to determine if they can serve as metal-free organic dyes in the use of dye-sensitized solar cells (DSSCs). To understand the trends in the relative efficiencies of the investigated compounds as dyes in DSSCs, their electron contributions, hole contributions, and electron–hole overlaps for each respective atom and fragment within the molecule were analyzed with a particular focus on the electron densities on the anchoring segments. As transition density matrices (TDM) provide details about the departure of each electron from its corresponding hole during excitations, which results in charge transfer (CT), the charge separation distance (Δr) between the electron and its corresponding hole was studied, in addition to the degree of electron–hole overlap (Λ). The latter, single-point excitation energy of each electron, the percentage electron contribution to the anchoring segments of each compound, the incident-photon-conversion-efficiency (IPCE), charge recombination, light harvesting efficiency (LHE), electron injection (Φinj), and charge collection efficiency (ncollect) were then compared to Δr to determine whether the expected relationships hold. Moreover, parameters such as diffusion constant (Dπ) and electron lifetime (t), amongst others, were also used to describe electron excitation processes. Since IPCE is the key parameter in determining the efficiency, O3 was found to be the best dye due to its highest value.
The Importance of Introducing Zero- and Low-Carbon Solutions in Urban Bus Transport
Authors: Drożdż Wojciech , Rosa Grażyna , Pomianowski Artur
Published: July 2022

The aim of this paper is to find the most important aspects of introducing zero-emission and low-carbon solutions in urban bus transport, as well as their availability. Beyond the economic aspects related to energy sources and consumption, the paper focuses on their impact on people and the environment. There is also an attempt to estimate the scale of the impact of changes in the structure of the energy sources of buses in urban transport compared with all the means of transport operating in a given area, as well as a justification for these changes. We also conducted a survey of bus passengers to check their awareness of the changes taking place and their attitude towards them.
Optimal Allocation Method of Source and Storage Capacity of PV-Hydrogen Zero Carbon Emission Microgrid Considering the Usage Cost of Energy Storage Equipment
Authors: Zhao Hongshan , Xu Junyang , Xu Kunyu , Sun Jingjie , Wang Yufeng
Published: July 2022

Aiming to meet the low-carbon demands of power generation in the process of carbon peaking and carbon neutralization, this paper proposes an optimal PV-hydrogen zero carbon emission microgrid. The light–electricity–hydrogen coupling utilization mode is adopted. The hydrogen-based energy system replaces the carbon-based energy system to realize zero carbon emissions. Firstly, the mathematical models of photovoltaic, hydrogen and electric energy storage systems in a microgrid are built. Then, the optimal allocation model of the microgrid source storage capacity is established, and a scheduling strategy considering the minimum operational cost of energy storage equipment is proposed. The priority of equipment output is determined by comparing the operational costs of the hydrogen energy storage system and the electric energy storage system. Finally, the proposed scheme is compared with the scheduling scheme of the battery priority and the hydrogen energy system priority in an actual microgrid. It is verified that the scheme can ensure stable power-generating, zero carbon operation of a microgrid system while reducing the total annual power costs by 9.8% and 25.1%, respectively.
Improving the Aromatic Profile of Plant-Based Meat Alternatives: Effect of Myoglobin Addition on Volatiles
Authors: Devaere Jolien , De Winne Ann , Dewulf Lore , Fraeye Ilse , Šoljić Irena , Lauwers Elsa , de Jong Andy , Sanctorum Hermes
Published: July 2022

Market demand for palatable plant-based meat alternatives is on the rise. One of the challenges is formulating products with sensorial characteristics similar to conventional meat. In this study, the effect of myoglobin on the aromatic profile of plant-based meat alternatives was assessed. Plant-based burgers were made with soy-textured protein, supplemented with three levels of myoglobin (0, 0.5 and 1.0%, the latter two mimicking endogenous myoglobin levels in meat), and grilled for 12 min at 250 °C. To evaluate the aromatic profile of the compounds, raw and grilled samples were subjected to headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS). Principal component analysis (PCA) analysis was then performed to visualize the interaction between grilling and myoglobin addition, and the effect exerted on the resulting aromatic profile. Myoglobin significantly affected several classes of volatile compounds, either by itself or in conjunction with grilling. A notable increase in aldehydes and a decrease in hydrocarbons were noted after adding myoglobin. As expected, an increase in pyrazines was observed after grilling. The results suggest myoglobin positively influences the aromatic profile of plant-based meat alternatives, contributing to a profile closer to the one of conventional meat.
The Effect of Different Freshness of Raw Material on Lipid Quality and Sensory Acceptance of Canned Sardines
Authors: Reblová Zuzana , Aubourg Santiago P. , Pokorný Jan
Published: July 2022

We studied how storing fresh sardines (Sardina pilchardus) on ice for 0–15 days would affect lipid quality and sensory acceptance after the sardines were later canned. Average moisture and diacylglycerol contents showed a decreasing trend during storage time for sardines stored for to 0–10 days and an increasing trend for samples stored for 13–15 days. Total lipid and triacylglycerol average values increased with storage time of 0–10 days. In contrast, sardines stored for 13–15 days showed decreased values of lipids and triacylglycerols. Increased storage times also led to increased average saturated fatty acid (STFA) content and browning and decreased polyunsaturated fatty acid (PUFA) values and PUFA/STFA and ω3/ω6 ratios. Notably, the effect of storage time on PUFA/STFA and ω3/ω6 ratios and browning development was found significant (p < 0.05). Sensory descriptors revealed only slight quality differences with previous storage on ice for 0-10 days. In contrast, a substantial (p < 0.05) decrease (appearance and texture) was detected in samples corresponding to a 13–15-day period, such samples being considered unacceptable. Storage on ice not exceeding 10 days is recommended for sardines before being shipped to canneries for further processing. Furthermore, the use of efficient storage including preserving technologies would be desirable.
Biochemical and Molecular Characteristics of a Novel Hyaluronic Acid Lyase from Citrobacter freundii
Authors: Li Xinyue , Li Fang , Ma Junhao , Li Mingjun , Lei Xi , Tang Xianghua , Wu Qian , Huang Zunxi , Zhang Rui
Published: July 2022

The Gram-negative strain of Citrobacter freundii, YNLX, has the ability to degrade hyaluronic acid. In this study, we expressed a C. freundii hyaluronic acid lyase, from polysaccharide lyase family 8, in Escherichia coli. The purified recombinant enzyme (rHynACF8) showed a substantially higher cleavage activity of hyaluronic acid than chondroitin sulfate. We found that its optimal pH and temperature are 5.5 and 35 °C, respectively. In addition, the enzyme activity was not notably affected by most metal ions. Km and kcat of rHynACF8 towards HA were 1.5 ± 0.01 mg/mL and 30.9 ± 0.5 /s, respectively. rHynACF8 is an endo-acting enzyme. Its cleavage products had dramatically increased antioxidant activity than hyaluronic acid in vitro (p < 0.001). As the molecular weight of hyaluronic acid decreased, the intramolecular interactions among antioxidant functional groups were removed; in the process of the cracking reaction, new double bonds formed and conjugated with the carbonyl group. We presumed that the structural change is the critical factor influencing antioxidant capacity. Overall, we found that rHynACF8 from Gram-negative bacteria with metal ion resistance, indicated the relationship between the function and structure of its antioxidant cleavage product.
The State of the Art of Cooperative and Connected Autonomous Vehicles from the Future Mobility Management Perspective: A Systematic Review
Authors: Alam Md. Saniul , Georgakis Panagiotis
Published: July 2022

Cooperative and connected autonomous vehicles (CCAVs) are considered to be a promising solution for addressing congestion and other operational deficiencies, as part of a holistic future mobility management framework. As a result, a significant number of studies have recently been published on this topic. From the perspective of future mobility management, this review paper discusses three themes, which are traffic management, network performance, and mobility management, including congestion, and incident detection using the PRISMA methodology. Three databases were considered for this study, and peer-reviewed primary studies were selected that were published within the last 10 years in the English language, focusing on CCAV in the context of the future transportation and mobility management perspective. For synthesis and interpretation, like-for-like comparisons were made among studies; it was found that extensive research-supported information is required to ensure a smooth transition from conventional vehicles to the CCAVs regime, to achieve the projected traffic and environmental benefits. Research investigations are ongoing to optimize these benefits and associated goals via the setting of different models and simulations. The tools and technologies for the testing and simulation of CCAV were found to have limited capacity. Following the review of the current state-of-the-art, recommendations for future research have been discussed. The most notable is the need for large-scale simulations to understand the impact of CCAVs beyond corridor-based and small-scale networks, the need for understanding the interactions between the drivers of CCAVs and traffic management centers, and the need to assess the technological transition, as far as infrastructure systems are concerned, that is necessary for the progressive penetration of CCAVs into traffic streams.
Diagnostic Accuracy of the Overlapping Infinity Loops, Wire Cube, and Clock Drawing Tests in Subjective Cognitive Decline, Mild Cognitive Impairment and Dementia
Authors: Costa Sigourney , St George Rebecca J. , McDonald James Scott , Wang Xinyi , Alty Jane
Published: July 2022

Figure drawing tasks are commonly used standalone or as part of broader screening tests to detect cognitive impairment. Only one study has compared the classification accuracy of three common drawing tasks—overlapping infinity loops, wire cube, and the clock drawing task (CDT)—in mild cognitive impairment (MCI) and dementia, but age and education, which impact performance, were not accounted for. We replicated the research, adjusting for age and education and, for the first time, assessed subjective cognitive decline (SCD) too. Participants were recruited from the Tasmanian ISLAND Cognitive Clinic and healthy controls from a community sample. All participants completed the three figure drawing tasks. The clinic patients were categorised according to interdisciplinary consensus diagnosis. Binomial logistic regression and area under ROC curves (AUC) were calculated to determine the discriminatory ability of each drawing task. Overall, 112 adults were recruited; 51 had normal cognition (NC), 21 SCD, 24 MCI, and 16 had dementia. The infinity loops test did not discriminate any of the groups, casting some doubt on its usefulness. The wire cube discriminated NC from dementia (AUC 0.7; p < 0.05). The CDT discriminated NC from dementia (AUC 0.77; p < 0.01), NC from cognitive impairment (dementia + MCI; AUC 0.59; p < 0.05), and MCI from dementia (AUC 0.76; p < 0.01). None of the tests discriminated NC from MCI or NC from SCD. The CDT was the most discriminatory test, followed by the wire cube. This may help guide clinicians who often choose just one figure drawing task due to time constraints or patient fatigue.
Effectiveness of Digital Flipped Learning Evidence-Based Practice on Nurses’ Knowledge, Attitude, and Practice: A Quasi-Experimental Trial
Authors: Chao Wen-Yi , Huang Li-Chi , Hung Hung-Chang , Hung Shih-Chang , Chuang Tzung-Fang , Yeh Li-Yueh , Tseng Hui-Chen
Published: July 2022

Background: Evidence-based care has become critical in raising the quality of medical facilities. The implementation of evidence-based practice helps medical practitioners make better clinical decisions. Objective: The objective of this study was to investigate whether the innovative flipped teaching model could be as effective as the conventional teaching model in terms of knowledge, attitude, and practice and to confirm the continuous effect. Design: A quasi-experimental design using the flipped and conventional learning groups concurrently with repeat measurements was used. Setting: The setting was a 475-bed regional teaching hospital in Taiwan, from March to July 2020. Participants: The study included 114 licensed nurses who had worked longer than three months, with 57 participants each in two groups. Methods: The participants were assigned to two groups using a block randomization method. All participants completed questionnaires related to knowledge, attitude, and practice of EBP at four-time points: pre-test (T0) and immediately after intervention (T1), at month 1 (T2), and at month 3 (T3). Analysis of repeated generalized estimating equations was used. Results: The flipped and conventional learning groups had significant differences in knowledge, attitude, and practice at the T0 and T1 (p < 0.05). The flipped group was higher than the conventional group at T3 in the knowledge score (p = 0.001) and lower than the conventional group at T2 in the attitude score (p = 0.010). There were no significant differences between the two groups’ practice scores at different time points. There were no significantly different score changes for knowledge, attitude, and practice (p > 0.05). The interaction term only at T3 vs. T0 in the knowledge score was slightly different (p = 0.049) in primary outcome. Conclusion: The intervention methods of both groups were effective. Flipped learning is more flexible and has more time for discussion, which nurses favor. Under the policy promoted in the hospital, EBP combined with the nursing advancement system was standardized, and conventional learning also improved the learning effect.
Aqua Walking as an Appropriate and Healthy Winter and Summer Physical Practice? An Exploratory Study
Authors: Bailly Mélina , Fillon Alicia , Bonjean Line , Lucas Dominique , Kabani Catherine , Chipon Sophie , Pereira Bruno , Duclos Martine , Verney Julien , Thivel David
Published: July 2022

(1) Background: Aqua-walking in a natural environment is a health promoting physical activity that is gaining popularity and appropriate for a variety of populations, however, to date, there is little scientific evidence supporting the efficacy and safety of this activity for older adults. The objective was to propose a preliminary exploration of psychometric and metabolic responses to an acute Aqua walking session either during winter or summer in older adults Aqua walking exercisers. (2) Methods: Heart rate, body temperature, glycemia, and blood pressure were monitored in 37 (30 women, 7 men) participants aged 52 to 83 years old in two Aqua walking sessions (water at 13 °C and 18.5 °C, respectively). Anthropometry (body weight, waist, and hip circumferences), body composition, physical activity level, sedentary time, sleep quality, quality of life, physical self-perception, and perceived health and feelings on various parameters were also assessed. (3) Results: Present results revealed a greater quality of life, physical self-perception, and perceived health in aging Aqua walking exercisers compared to those found in the literature in younger populations. None of the metabolic or psychometric measurements were found to be different between classically calibrated Aqua walking sessions performed in winter compared to summer. By contrast, there was a time effect during the sessions for body temperature (p < 0.001), SBP (p = 0.17), perceived mental and physical well-being (p = 0.006 and p < 0.001, respectively), and anxiety (p < 0.001). Leg discomfort also showed a time effect (p = 0.0009) and interaction effect (p = 0.025). (4) Conclusion: Aqua walking appears here to be an accessible practice that can be performed all year long with a range of physical and mental benefits for older adults. Future studies should investigate the metabolic responses of Aqua walking in different populations.
Postischemic Neuroprotection of Aminoethoxydiphenyl Borate Associates Shortening of Peri-Infarct Depolarizations
Authors: Fernández-Serra Rocío , Martínez-Alonso Emma , Alcázar Alberto , Chioua Mourad , Marco-Contelles José , Martínez-Murillo Ricardo , Ramos Milagros , Guinea Gustavo V. , González-Nieto Daniel
Published: July 2022

Brain stroke is a highly prevalent pathology and a main cause of disability among older adults. If not promptly treated with recanalization therapies, primary and secondary mechanisms of injury contribute to an increase in the lesion, enhancing neurological deficits. Targeting excitotoxicity and oxidative stress are very promising approaches, but only a few compounds have reached the clinic with relatively good positive outcomes. The exploration of novel targets might overcome the lack of clinical translation of previous efficient preclinical neuroprotective treatments. In this study, we examined the neuroprotective properties of 2-aminoethoxydiphenyl borate (2-APB), a molecule that interferes with intracellular calcium dynamics by the antagonization of several channels and receptors. In a permanent model of cerebral ischemia, we showed that 2-APB reduces the extent of the damage and preserves the functionality of the cortical territory, as evaluated by somatosensory evoked potentials (SSEPs). While in this permanent ischemia model, the neuroprotective effect exerted by the antioxidant scavenger cholesteronitrone F2 was associated with a reduction in reactive oxygen species (ROS) and better neuronal survival in the penumbra, 2-APB did not modify the inflammatory response or decrease the content of ROS and was mostly associated with a shortening of peri-infarct depolarizations, which translated into better cerebral blood perfusion in the penumbra. Our study highlights the potential of 2-APB to target spreading depolarization events and their associated inverse hemodynamic changes, which mainly contribute to extension of the area of lesion in cerebrovascular pathologies.
SPR-Based Detection of ASF Virus in Cells
Authors: Capo Alessandro , Calabrese Alessia , Frant Maciej , Walczak Marek , Szczotka-Bochniarz Anna , Manessis Georgios , Bossis Ioannis , Staiano Maria , D’Auria Sabato , Varriale Antonio
Published: July 2022

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 103 HAD50/mL.
Hepatic Expression of the Na+-Taurocholate Cotransporting Polypeptide Is Independent from Genetic Variation
Authors: Tremmel Roman , Nies Anne T. , van Eijck Barbara A. C. , Handin Niklas , Haag Mathias , Winter Stefan , Büttner Florian A. , Kölz Charlotte , Klein Franziska , Mazzola Pascale , Hofmann Ute , Klein Kathrin , Hoffmann Per , Nöthen Markus M. , Gaugaz Fabienne Z. , Artursson Per , Schwab Matthias , Schaeffeler Elke
Published: July 2022

The hepatic Na+-taurocholate cotransporting polypeptide NTCP/SLC10A1 is important for the uptake of bile salts and selected drugs. Its inhibition results in increased systemic bile salt concentrations. NTCP is also the entry receptor for the hepatitis B/D virus. We investigated interindividual hepatic SLC10A1/NTCP expression using various omics technologies. SLC10A1/NTCP mRNA expression/protein abundance was quantified in well-characterized 143 human livers by real-time PCR and LC-MS/MS-based targeted proteomics. Genome-wide SNP arrays and SLC10A1 next-generation sequencing were used for genomic analyses. SLC10A1 DNA methylation was assessed through MALDI-TOF MS. Transcriptomics and untargeted metabolomics (UHPLC-Q-TOF-MS) were correlated to identify NTCP-related metabolic pathways. SLC10A1 mRNA and NTCP protein levels varied 44-fold and 10.4-fold, respectively. Non-genetic factors (e.g., smoking, alcohol consumption) influenced significantly NTCP expression. Genetic variants in SLC10A1 or other genes do not explain expression variability which was validated in livers (n = 50) from The Cancer Genome Atlas. The identified two missense SLC10A1 variants did not impair transport function in transfectants. Specific CpG sites in SLC10A1 as well as single metabolic alterations and pathways (e.g., peroxisomal and bile acid synthesis) were significantly associated with expression. Inter-individual variability of NTCP expression is multifactorial with the contribution of clinical factors, DNA methylation, transcriptional regulation as well as hepatic metabolism, but not genetic variation.
Polyphenol-Enriched Composite Bone Regeneration Materials: A Systematic Review of In Vitro Studies
Authors: Checinska Kamila , Checinski Maciej , Cholewa-Kowalska Katarzyna , Sikora Maciej , Chlubek Dariusz
Published: July 2022

One of the possible alternatives for creating materials for the regeneration of bone tissue supporting comprehensive reconstruction is the incorporation of active substances whose controlled release will improve this process. This systematic review aimed to identify and synthesize in vitro studies that assess the suitability of polyphenolics as additives to polymer-ceramic composite bone regeneration materials. Data on experimental studies in terms of the difference in mechanical, wettability, cytocompatibility, antioxidant and anti-inflammatory properties of materials were synthesized. The obtained numerical data were compiled and analyzed in search of percentage changes of these parameters. The results of the systematic review were based on data from forty-six studies presented in nineteen articles. The addition of polyphenolic compounds to composite materials for bone regeneration improved the cytocompatibility and increased the activity of early markers of osteoblast differentiation, indicating a high osteoinductive potential of the materials. Polyphenolic compounds incorporated into the materials presumably give them high antioxidant properties and reduce the production of reactive oxygen species in macrophage cells, implying anti-inflammatory activity. The evidence was limited by the number of missing data and the heterogeneity of the data.
Loss of TRIM67 Attenuates the Progress of Obesity-Induced Non-Alcoholic Fatty Liver Disease
Authors: Huang Chao , Wei Xiaoli , Luo Qihui , Xia Yu , Pan Ting , He Junbo , Jahangir Asad , Jia Lanlan , Liu Wentao , Zou Yuanfeng , Li Lixia , Guo Hongrui , Geng Yi , Chen Zhengli
Published: July 2022

Obesity is considered as a major cause for the development and progress of non-alcoholic fatty liver disease (NAFLD), which is one of the most prevalent chronic liver diseases worldwide. However, molecular mechanisms that implicate in obesity-driven pathophysiology of NAFLD are not well defined. Here, we report a tripartite motif (TRIM) protein family member—TRIM67—that is hardly expressed in liver but is inducible on obese conditions. Enhanced expression of TRIM67 activates hepatic inflammation to disturb lipid metabolic homeostasis and promote the progress of NAFLD induced by obesity, while the deficiency in TRIM67 is protective against these pathophysiological processes. Finally, we show that the important transcription coactivator PGC-1α implicates in the response of hepatic TRIM67 to obesity.
Complete Reaction Phenotyping of Propranolol and 4-Hydroxypropranolol with the 19 Enzymes of the Human UGT1 and UGT2 Families
Authors: Yang Fan , Liu Sijie , Wolber Gerhard , Bureik Matthias , Parr Maria Kristina
Published: July 2022

Propranolol is a competitive non-selective beta-receptor antagonist that is available on the market as a racemic mixture. In the present study, glucuronidation of propranolol and its equipotent phase I metabolite 4-hydroxypropranolol by all 19 members of the human UGT1 and UGT2 families was monitored. UGT1A7, UGT1A9, UGT1A10 and UGT2A1 were found to glucuronidate propranolol, with UGT1A7, UGT1A9 and UGT2A1 mainly acting on (S)-propranolol, while UGT1A10 displays the opposite stereoselectivity. UGT1A7, UGT1A9 and UGT2A1 were also found to glucuronidate 4-hydroxypropranolol. In contrast to propranolol, 4-hydroxypropranolol was found to be glucuronidated by UGT1A8 but not by UGT1A10. Additional biotransformations with 4-methoxypropanolol demonstrated different regioselectivities of these UGTs with respect to the aliphatic and aromatic hydroxy groups of the substrate. Modeling and molecular docking studies were performed to explain the stereoselective glucuronidation of the substrates under study.
Far-Red Light Coordinates the Diurnal Changes in the Transcripts Related to Nitrate Reduction, Glutathione Metabolism and Antioxidant Enzymes in Barley
Authors: Balogh Eszter , Kalapos Balázs , Ahres Mohamed , Boldizsár Ákos , Gierczik Krisztián , Gulyás Zsolt , Gyugos Mónika , Szalai Gabriella , Novák Aliz , Kocsy Gábor
Published: July 2022

Spectral quality, intensity and period of light modify many regulatory and stress signaling pathways in plants. Both nitrate and sulfate assimilations must be synchronized with photosynthesis, which ensures energy and reductants for these pathways. However, photosynthesis is also a source of reactive oxygen species, whose levels are controlled by glutathione and other antioxidants. In this study, we investigated the effect of supplemental far-red (735 nm) and blue (450 nm) lights on the diurnal expression of the genes related to photoreceptors, the circadian clock, nitrate reduction, glutathione metabolism and various antioxidants in barley. The maximum expression of the investigated four photoreceptor and three clock-associated genes during the light period was followed by the peaking of the transcripts of the three redox-responsive transcription factors during the dark phase, while most of the nitrate and sulfate reduction, glutathione metabolism and antioxidant-enzyme-related genes exhibited high expression during light exposure in plants grown in light/dark cycles for two days. These oscillations changed or disappeared in constant white light during the subsequent two days. Supplemental far-red light induced the activation of most of the studied genes, while supplemental blue light did not affect or inhibited them during light/dark cycles. However, in constant light, several genes exhibited greater expression in blue light than in white and far-red lights. Based on a correlation analysis of the gene expression data, we propose a major role of far-red light in the coordinated transcriptional adjustment of nitrate reduction, glutathione metabolism and antioxidant enzymes to changes of the light spectrum.
Chloride Binding Properties of a Macrocyclic Receptor Equipped with an Acetylide Gold(I) Complex: Synthesis, Characterization, Reactivity, and Cytotoxicity Studies
Authors: Rivoli Andrea , Aragay Gemma , Gimeno María Concepción , Ballester Pablo
Published: July 2022

In this work, we report the synthesis and characterization of a mono-nuclear “two wall” aryl-extended calix[4]pyrrole receptor (2Au) decorated with an acetylide-gold(I)-PTA complex at its upper rim. We describe the 1H NMR titration experiments of 2Au and its “two wall” aryl-extended calix[4]pyrrole synthetic precursors: the non-symmetric mono-iodo-mono-ethynyl 2 and the symmetric bis-iodo 3 with TBACl in dichloromethane and acetone solution. In acetone solution, we use isothermal titration calorimetry (ITC) experiments to thermodynamically characterize the formed 1:1 chloride complexes and perform pair-wise competitive binding experiments. In both solvents, we measured a decrease in the binding constant of the mono-nuclear 2Au complex for chloride compared to the parent mono-iodo-mono-ethynyl 2. In turn, receptor 2 also shows a reduction in binding affinity for chloride compared to its precursor bis-iodo calix[4]pyrrole 3. The free energy differences (∆G) of the 1:1 chloride complexes cannot be exclusively attributed to their dissimilar electrostatic surface potential values either at the center of the meso-phenyl wall or its para-substituent. We conclude that solvation/desolvation processes play an important role in the stabilization of the chloride complexes. In acetone solution and in the presence of TBACl, 6Au, a reference compound for the acetylide Au(I)•PTA unit, produces a bis(alkynyl)gold(I) anionic complex [7Au]−. Thus, the observation of two separate sets of signals for the bound aromatic calix[4]pyrrole protons, when more than 1 equiv. of the salt is added, is assigned to the formation of the chloride complexes of 2Au and of the “in situ” formed calix[4]pyrrole anionic dimer [8Au]−. Finally, preliminary data obtained in cell viability assays of 2Au and 6Au with human cancer cells lines assign them with moderate activities showing that the calix[4]pyrrole unit is not relevant.
The Role of Epicardial Adipose Tissue in the Development of Atrial Fibrillation, Coronary Artery Disease and Chronic Heart Failure in the Context of Obesity and Type 2 Diabetes Mellitus: A Narrative Review
Authors: Krishnan Anirudh , Sharma Harman , Yuan Daniel , Trollope Alexandra F. , Chilton Lisa
Published: July 2022

Cardiovascular diseases (CVDs) are a significant burden globally and are especially prevalent in obese and/or diabetic populations. Epicardial adipose tissue (EAT) surrounding the heart has been implicated in the development of CVDs as EAT can shift from a protective to a maladaptive phenotype in diseased states. In diabetic and obese patients, an elevated EAT mass both secretes pro-fibrotic/pro-inflammatory adipokines and forms intramyocardial fibrofatty infiltrates. This narrative review considers the proposed pathophysiological roles of EAT in CVDs. Diabetes is associated with a disordered energy utilization in the heart, which promotes intramyocardial fat and structural remodeling. Fibrofatty infiltrates are associated with abnormal cardiomyocyte calcium handling and repolarization, increasing the probability of afterdepolarizations. The inflammatory phenotype also promotes lateralization of connexin (Cx) proteins, undermining unidirectional conduction. These changes are associated with conduction heterogeneity, together creating a substrate for atrial fibrillation (AF). EAT is also strongly implicated in coronary artery disease (CAD); inflammatory adipokines from peri-vascular fat can modulate intra-luminal homeostasis through an “outside-to-inside” mechanism. EAT is also a significant source of sympathetic neurotransmitters, which promote progressive diastolic dysfunction with eventual cardiac failure. Further investigations on the behavior of EAT in diabetic/obese patients with CVD could help elucidate the pathogenesis and uncover potential therapeutic targets.
Which Explanatory Variables Contribute to the Classification of Good Visual Acuity over Time in Patients with Branch Retinal Vein Occlusion with Macular Edema Using Machine Learning?
Authors: Matsui Yoshitsugu , Imamura Kazuya , Chujo Shinichiro , Mase Yoko , Matsubara Hisashi , Sugimoto Masahiko , Kawanaka Hiroharu , Kondo Mineo
Published: July 2022

This study’s goal is to determine the accuracy of a linear classifier that predicts the prognosis of patients with macular edema (ME) due to a branch retinal vein occlusion during the maintenance phase of antivascular endothelial growth factor (anti-VEGF) therapy. The classifier was created using the clinical information and optical coherence tomographic (OCT) findings obtained up to the time of the first resolution of ME. In total, 66 eyes of 66 patients received an initial intravitreal injection of anti-VEGF followed by repeated injections with the pro re nata (PRN) regimen for 12 months. The patients were divided into two groups: those with and those without good vision during the PRN phase. The mean AUC of the classifier was 0.93, and the coefficients of the explanatory variables were: best-corrected visual acuity (BCVA) at baseline was 0.66, BCVA at first resolution of ME was 0.51, age was 0.21, the average brightness of the ellipsoid zone (EZ) was −0.12, the intactness of the external limiting membrane (ELM) was −0.14, the average brightness of the ELM was −0.17, the brightness value of EZ was −0.17, the area of the outer segments of the photoreceptors was −0.20, and the intactness of the EZ was −0.24. This algorithm predicted the prognosis over time for individual patients during the PRN phase.
Clinical Outcome and Comparison of Regenerative and Apexification Intervention in Young Immature Necrotic Teeth—A Systematic Review and Meta-Analysis
Authors: Panda Pratima , Mishra Lora , Govind Shashirekha , Panda Saurav , Lapinska Barbara
Published: July 2022

This systematic review aimed to evaluate interventions individually and compare the clinical outcome of young, immature teeth treated with regenerative endodontic therapy (RET) and apexification procedure. The protocol was registered with PROSPERO (International Prospective Register of Systematic Reviews), bearing the registration number CRD42021230284. A bibliographic search in the biomedical databases was conducted in four databases—PubMed, CENTRAL, EMBASE and ProQuest—using searching keywords and was limited to studies published between January 2000 and April 2022 in English. The search was supplemented by manual searching, citation screening and scanning of all reference lists of selected paper. The study selection criteria were randomized clinical trial, prospective clinical studies and observational studies. The search found 32 eligible articles, which were included in the study. The quality assessment of the studies was performed using the Cochrane risk of bias tool for randomized control trials and non-randomized clinical studies. The meta-analysis was performed using Review Manager software (REVMAN, version 5). The results indicated that a clinicians’ MTA apexification procedure was more successful compared to calcium hydroxide. In RET, apical closure and overall success rate is statistically same for both apical platelet concentrates (APCs) and blood clots (BC). Both interventions have similar survival rates; however, RET should be preferred in cases where the root development is severely deficient, there is insufficient dentine and the tooth’s prognosis is hopeless even with an apexification procedure.
The Natural History of Esophageal “Absent Contractility” and Its Relationship with Rheumatologic Diseases: A Multi-Center Case–Control Study
Authors: Cohen Daniel , Dickman Ram , Bermont Anton , Richter Vered , Shirin Haim , Mari Amir
Published: July 2022

(1) Background: Absent contractility (AC) is an esophageal motility disorder defined as a normal integrated relaxation pressure with 100% failed peristalsis. We sought to clarify the natural history of this disorder and its relationship with rheumatologic diseases, such as systemic sclerosis (scleroderma). (2) Methods: We retrospectively identified patients with AC based on high-resolution manometry findings at three referral institutions and then matched them with controls with esophageal complaints who had normal manometries. (3) Results: Seventy-four patients with AC were included (mean age 56 years; 69% female). Sixteen patients (21.6%) had a rheumatologic disease. Compared to controls, patients with AC were significantly more likely to present with heartburn, dysphagia, vomiting, and weight loss. During follow-up, they were also more likely to be seen by a gastroenterologist, be diagnosed with gastroesophageal reflux disease, take a proton pump inhibitor, and undergo repeat upper endoscopies. No AC patients developed a new rheumatologic disease during follow-up. No significant differences were noted in the clinical presentation or course of AC patients with rheumatologic disease compared to those without. (4) Conclusions: Patients with AC have more esophageal symptoms and require more intense gastrointestinal follow-up than controls. Only a minority of patients with AC have underlying rheumatologic disease. Those without rheumatologic disease at baseline did not subsequently develop one, suggesting that a rheumatologic evaluation is likely unnecessary. The clinical course of AC in patients with rheumatologic disease and those without appears to be similar.
Palliative Care for Patients with Kidney Disease
Authors: Lanini Iacopo , Samoni Sara , Husain-Syed Faeq , Fabbri Sergio , Canzani Filippo , Messeri Andrea , Mediati Rocco Domenico , Ricci Zaccaria , Romagnoli Stefano , Villa Gianluca
Published: July 2022

Interest in palliative care has increased in recent times, particularly in its multidisciplinary approach developed to meet the needs of patients with a life-threatening disease and their families. Although the modern concept of palliative simultaneous care postulates the adoption of these qualitative treatments early on during the life-threatening disease (and potentially just after the diagnosis), palliative care is still reserved for patients at the end of their life in most of the clinical realities, and thus is consequently mistaken for hospice care. Patients with acute or chronic kidney disease (CKD) usually experience poor quality of life and decreased survival expectancy and thus may benefit from palliative care. Palliative care requires close collaboration among multiple health care providers, patients, and their families to share the diagnosis, prognosis, realistic treatment goals, and treatment decisions. Several approaches, such as conservative management, extracorporeal, and peritoneal palliative dialysis, can be attempted to globally meet the needs of patients with kidney disease (e.g., physical, social, psychological, or spiritual needs). Particularly for frail patients, pharmacologic management or peritoneal dialysis may be more appropriate than extracorporeal treatment. Extracorporeal dialysis treatment may be disproportionate in these patients and associated with a high burden of symptoms correlated with this invasive procedure. For those patients undergoing extracorporeal dialysis, individualized goal setting and a broader concept of adequacy should be considered as the foundations of extracorporeal palliative dialysis. Interestingly, little evidence is available on palliative and end of life care for acute kidney injury (AKI) patients. In this review, the main variables influencing medical decision-making about palliative care in patients with kidney disease are described, as well as the different approaches that can fulfill the needs of patients with CKD and AKI.
Enhanced Teaching Learning-Based Algorithm for Fuel Costs and Losses Minimization in AC-DC Systems
Authors: Sarhan Shahenda , Shaheen Abdullah M. , El-Sehiemy Ragab A. , Gafar Mona
Published: July 2022

The Teaching Learning-Based Algorithm (TLBA) is a powerful and effective optimization approach. TLBA mimics the teaching-learning process in a classroom, where TLBA’s iterative computing process is separated into two phases, unlike standard evolutionary algorithms and swarm intelligence algorithms, and each phase conducts an iterative learning operation. Advanced technologies of Voltage Source Converters (VSCs) enable greater active and reactive power regulation in these networks. Various objectives are addressed for optimal energy management, with the goal of attaining economic and technical advantages by decreasing overall production fuel costs and transmission power losses in AC-DC transmission networks. In this paper, the TLBA is applied for various sorts of nonlinear and multimodal functioning of hybrid alternating current (AC) and multi-terminal direct current (DC) power grids. The proposed TLBA is evaluated on modified IEEE 30-bus and IEEE 57-bus AC-DC networks and compared to other published methods in the literature. Numerical results demonstrate that the proposed TLBA has great effectiveness and robustness indices over the others. Economically, the reduction percentages of 13.84 and 21.94% are achieved for the IEEE 30-bus and IEEE 57-bus test systems when the fuel costs are minimized. Technically, significant improvement in the transmission power losses with reduction 28.01% and 69.83%, are found for the IEEE 30-bus and IEEE 57-bus test system compared to the initial case. Nevertheless, TLBA has faster convergence, higher quality for the final optimal solution, and more power for escaping from convergence to local optima compared to other published methods in the literature.
Spectral Decompositions of Gramians of Continuous Stationary Systems Given by Equations of State in Canonical Forms
Authors: Yadykin Igor
Published: July 2022

The application of transformations of the state equations of continuous linear and bilinear systems to the canonical form of controllability allows one to simplify the computation of Gramians of these systems. In this paper, we develop the method and obtain algorithms for computation of the controllability and observability Gramians of continuous linear and bilinear stationary systems with many inputs and one output, based on the method of spectral expansion of the Gramians and the iterative method for computing the bilinear systems Gramians. An important feature of the concept is the idea of separability of the Gramians expansion: separate computation of the scalar and matrix parts of the spectral Gramian expansion reduces the sub-Gramian matrices computation to calculation of numerical sequences of their elements. For the continuous linear systems with one output the method and the algorithm of the spectral decomposition of the controllability Gramian are developed in the form of Xiao matrices. Analytical expressions for the diagonal elements of the Gramian matrices are obtained, and by making use of which the rest of the elements can be calculated. For continuous linear systems with many outputs the spectral decompositions of the Gramians in the form of generalized Xiao matrices are obtained, which allows us to significantly reduce the number of calculations. The obtained results are generalized for continuous bilinear systems with one output. Iterative spectral algorithms for computation of elements of Gramians of these systems are proposed. Examples are given that illustrate theoretical results.
Empirical Likelihood Ratio Tests for Homogeneity of Multiple Populations in the Presence of Auxiliary Information
Authors: Wu Ronghuo , Qin Yongsong
Published: July 2022

The empirical likelihood ratio test (ELRT) statistic is constructed for testing the homogeneity of several nonparametric populations in the presence of some auxiliary information. It is shown—under some regularity conditions and under the null hypothesis that all distribution functions of the populations are equal—that the asymptotic distribution of the ELRT is a chi-squared distribution. The proposed ELRT could be more powerful than the Kruskal–Wallis test, as extra information can be efficiently employed by ELRT. The advantage of ELRT over T&P (2006) is that researchers do not need to select approximately normal statistics for inter-group comparisons, and ELRT is more suitable for the multi-population consistency test with a small sample size.
How Do Pre-/In-Service Mathematics Teachers Reason for or against the Use of Digital Technology in Teaching?
Authors: Gonscherowski Peter , Rott Benjamin
Published: July 2022

The role and the availability of digital technology in society is growing, which is why educators need to increasingly more often decide which types of digital technology to integrate into their teaching and when to integrate them. Thus, those decision-making skills need to be developed and measured especially for pre-service mathematics teachers. Therefore, we conducted an explorative interview study to understand the portfolio of argumentation on whether to use digital technology in different teaching phases and what criteria are used when making those decisions. Our results are based on ten interviews with pre- and in-service mathematics teachers in Germany. The analysis shows that (1) different levels of argumentation can be distinguished and (2) there are indications that teachers need to be aware of digital technology when deciding whether or not to use digital technology in a teaching setting. In addition, (3) besides the teaching-phase perspective and the learner-perspective, we expanded current research by formalizing the educator-perspective in a list of decision criteria. The compiled list of decision criteria was theoretically validated through the literature. In combination with the applied teaching phase framework and taxonomy of digital technology the list could aid in the development of the decision-making skills and potentially could result in a more reflective use of digital technology by pre-service and in-service teachers.