bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNA 132-3p Is Upregulated in Laron Syndrome Patients and Controls Longevity Gene Expression
Authors: Yaron-Saminsky Danielle , Nagaraj Karthik , Sarfstein Rive , Laron Zvi , Pasmanik-Chor Metsada , Werner Haim
Published: November 2021

The growth hormone (GH)–insulin-like growth factor-1 (IGF1) endocrine axis is a central player in normal growth and metabolism as well as in a number of pathologies, including cancer. The GH–IGF1 hormonal system, in addition, has emerged as a major determinant of lifespan and healthspan. Laron syndrome (LS), the best characterized entity under the spectrum of the congenital IGF1 deficiencies, results from mutation of the GH receptor (GHR) gene, leading to dwarfism, obesity and other defects. Consistent with the key role of IGF1 in cellular proliferation, epidemiological studies have shown that LS patients are protected from cancer development. While reduced expression of components of the GH-IGF1 axis is associated with enhanced longevity in animal models, it is still unknown whether LS is associated with an increased lifespan. MicroRNAs (miRs) are endogenous short non-coding RNAs that regulate the expression of complementary mRNAs. While a number of miRs involved in the regulation of IGF components have been identified, no previous studies have investigated the differential expression of miRs in congenital IGF1 deficiencies. The present study was aimed at identifying miRs that are differentially expressed in LS and that might account for the phenotypic features of LS patients, including longevity. Our genomic analyses provide evidence that miR-132-3p was highly expressed in LS. In addition, we identified SIRT1, a member of the sirtuin family of histone deacetylases, as a target for negative regulation by miR-132-3p. The data was consistent with the notion that low concentrations of IGF1 in LS lead to elevated miR-132-3p levels, with ensuing reduction in SIRT1 gene expression. The impact of the IGF1-miR-132-3p-SIRT1 loop on aging merits further investigation.
Sulfonylurea Receptor 1 in Central Nervous System Injury: An Updated Review
Authors: Jha Ruchira M. , Rani Anupama , Desai Shashvat M. , Raikwar Sudhanshu , Mihaljevic Sandra , Munoz-Casabella Amanda , Kochanek Patrick M. , Catapano Joshua , Winkler Ethan , Citerio Giuseppe , Hemphill J. Claude , Kimberly W. Taylor , Narayan Raj , Sahuquillo Juan , Sheth Kevin N. , Simard J. Marc
Published: November 2021

Sulfonylurea receptor 1 (SUR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) protein superfamily, encoded by Abcc8, and is recognized as a key mediator of central nervous system (CNS) cellular swelling via the transient receptor potential melastatin 4 (TRPM4) channel. Discovered approximately 20 years ago, this channel is normally absent in the CNS but is transcriptionally upregulated after CNS injury. A comprehensive review on the pathophysiology and role of SUR1 in the CNS was published in 2012. Since then, the breadth and depth of understanding of the involvement of this channel in secondary injury has undergone exponential growth: SUR1-TRPM4 inhibition has been shown to decrease cerebral edema and hemorrhage progression in multiple preclinical models as well as in early clinical studies across a range of CNS diseases including ischemic stroke, traumatic brain injury, cardiac arrest, subarachnoid hemorrhage, spinal cord injury, intracerebral hemorrhage, multiple sclerosis, encephalitis, neuromalignancies, pain, liver failure, status epilepticus, retinopathies and HIV-associated neurocognitive disorder. Given these substantial developments, combined with the timeliness of ongoing clinical trials of SUR1 inhibition, now, another decade later, we review advances pertaining to SUR1-TRPM4 pathobiology in this spectrum of CNS disease—providing an overview of the journey from patch-clamp experiments to phase III trials.
Development of Concrete Incorporating Recycled Aggregates, Hydrated Lime and Natural Volcanic Pozzolan
Authors: Garcia-Troncoso Natividad , Xu Bowen , Probst-Pesantez Wilhenn
Published: November 2021

Recycling of construction and demolition waste is a central point of discussion throughout the world. The application of recycled concrete as partial replacement of mineral aggregates in concrete mixes is one of the alternatives in the reduction of pollution and savings in carbon emissions. The combined influence of the recycled crushed concrete, lime, and natural pozzolana on the mechanical and sustainable properties of concrete materials is firstly proposed in this study. In this research, unconventional construction materials are employed to produce concrete: the recycled crushed concrete is used as coarse aggregate, while lime and natural pozzolana are used as a partial replacement for cement. Substitutions of 10%, 20%, 50% of gravel are made with recycled aggregates, and 2%, 5%, 10% of cement with lime and natural pozzolan. Tests on the fresh and hardened properties, destructive (compressive strength) and non-destructive tests (sclerometer rebound and ultrasound) of mixtures are carried out. It is shown that the use of recycled materials can provide an increase in compressive strength of up to 34% with respect to conventional concrete. Life cycle cost and sustainability assessments indicate that concrete materials incorporating recycled aggregate possess good economic and environmental impacts.
Activation of the Host Immune Response in Hyphantria cunea (Drury) (Lepidoptera: Noctuidae) Induced by Serratia marcescens Bizio
Authors: Wang Zhiqiang , Feng Kai , Tang Fang , Xu Meng
Published: November 2021

Host–pathogen interactions are essential to our understanding of biological pesticides. Hyphantria cunea (Drury) is an important forest pest worldwide. The immune mechanism of the interaction between H. cunea and Serratia marcescens Bizio (SM1) is unclear. First, transcriptome sequencing and quantitative real-time PCR (qRT-PCR) analysis described the H. cunea immune response to SM1. A total of 234 immune-related differentially expressed genes (DEGs) were found. Many immune regulatory genes in three classical pathways were found. Antimicrobial peptides, including attacin B, cecropin A, gloverin, lebocin and diapausin, are involved in defending against SM1 challenge, and are mainly produced by Toll and immune deficiency (IMD) pathways. Some melanization genes were changed in H. cunea, which suggested that H. cunea melanization was activated by SM1. Furthermore, phagocytosis, autophagolysosome and apoptosis pathways in cellular immunity were activated in H. cunea against SM1. Finally, the expression patterns of 10 immune genes were analyzed systematically by qRT-PCR, and most of the genes were upregulated compared to the control. Our studies provide useful information about the immune response of H. cunea under the stress of SM1, which is important to understand how SM1 affects the immune system of H. cunea and provides new ideas to control H. cunea by using SM1.
The Association between Sarcoidosis and Ischemic Heart Disease—A Healthcare Analysis of a Large Israeli Population
Authors: Gonen Tal , Katz-Talmor Daphna , Amital Howard , Comaneshter Doron , Cohen Arnon D. , Tiosano Shmuel
Published: November 2021

(1) Background: Inflammation plays a pivotal role in atherosclerosis, and the association between chronic inflammatory states and ischemic heart disease (IHD) has been shown in several rheumatic diseases. Persistent inflammation might also be a risk factor for IHD in sarcoidosis patients. (2) Methods: Demographic and clinical data of 3750 sarcoidosis patients and 18,139 age- and sex-matched controls were retrieved from the database of Clalit Health Services, Israel’s largest healthcare organization. Variables associated with IHD were assessed by a logistic regression model. To assess for variables that were related to increased risk of all-cause mortality, the Cox proportional hazards method was used, and a log-rank test was performed for survival analysis. (3) Results: Both groups were composed of 64% females with a median age of 56 years. An association between sarcoidosis and IHD was demonstrated by a multivariate analysis (adjusted odds ratio (OR) 1.5; 95% confidence interval (CI) 1.36–1.66). Long-term follow-up revealed increased mortality among sarcoidosis patients: 561 (15%) deaths compared to 1636 (9%) deaths among controls (p < 0.001). Survival analysis demonstrated that sarcoidosis patients were also at increased risk for all-cause mortality compared to controls (multivariate model, adjusted HR 1.93; 95% CI 1.76–2.13).
Clinical Features of LMNA-Related Cardiomyopathy in 18 Patients and Characterization of Two Novel Variants
Authors: Ferradini Valentina , Cosma Joseph , Romeo Fabiana , De Masi Claudia , Murdocca Michela , Spitalieri Paola , Mannucci Sara , Parlapiano Giovanni , Di Lorenzo Francesca , Martino Annamaria , Fedele Francesco , Calò Leonardo , Novelli Giuseppe , Sangiuolo Federica , Mango Ruggiero
Published: November 2021

Dilated cardiomyopathy (DCM) refers to a spectrum of heterogeneous myocardial disorders characterized by ventricular dilation and depressed myocardial performance in the absence of hypertension, valvular, congenital, or ischemic heart disease. Mutations in LMNA gene, encoding for lamin A/C, account for 10% of familial DCM. LMNA-related cardiomyopathies are characterized by heterogeneous clinical manifestations that vary from a predominantly structural heart disease, mainly mild-to-moderate left ventricular (LV) dilatation associated or not with conduction system abnormalities, to highly pro-arrhythmic profiles where sudden cardiac death (SCD) occurs as the first manifestation of disease in an apparently normal heart. In the present study, we select, among 77 DCM families referred to our center for genetic counselling and molecular screening, 15 patient heterozygotes for LMNA variants. Segregation analysis in the relatives evidences other eight heterozygous patients. A genotype–phenotype correlation has been performed for symptomatic subjects. Lastly, we perform in vitro functional characterization of two novel LMNA variants using dermal fibroblasts obtained from three heterozygous patients, evidencing significant differences in terms of lamin expression and nuclear morphology. Due to the high risk of SCD that characterizes patients with lamin A/C cardiomyopathy, genetic testing for LMNA gene variants is highly recommended when there is suspicion of laminopathy.
Sildenafil Citrate Downregulates PDE5A mRNA Expression in Women with Recurrent Pregnancy Loss without Altering Angiogenic Factors—A Preliminary Study
Authors: Kniotek Monika , Roszczyk Aleksander , Zych Michał , Wrzosek Małgorzata , Szafarowska Monika , Zagożdżon Radosław , Jerzak Małgorzata
Published: November 2021

In our previous study, we showed that sildenafil citrate (SC), a selective PDE5A blocker, modulated NK cell activity in patients with recurrent pregnancy loss, which correlated with positive pregnancy outcomes. It was found that NK cells had a pivotal role in decidualization, angiogenesis, spiral artery remodeling, and the regulation of trophoblast invasion. Thus, in the current study, we determined the effects of SC on angiogenic factor expression and production, as well as idNK cell activity in the presence of nitric synthase blocker L-NMMA. Methods: NK cells (CD56+) were isolated from the peripheral blood of 15 patients and 15 fertile women on MACS columns and cultured in transformation media containing IL-15, TGF-β, and AZA—a methylation agent—for 7 days in hypoxia (94% N2, 1% O2, 5% CO2). Cultures were set up in four variants: (1) with SC, (2) without SC, (3) with NO, a synthase blocker, and (4) with SC and NO synthase blocker. NK cell activity was determined after 7 days of culturing as CD107a expression after an additional 4h of stimulation with K562 erythroleukemia cells. The expression of the PDE5A, VEGF-A, PIGF, IL-8, and RENBP genes was determined with quantitative real-time PCR (qRT-PCR) using TaqMan probes and ELISA was used to measure the concentrations of VEGF-A, PLGF, IL-8, Ang-I, Ang-II, IFN–γ proteins in culture supernatants after SC supplementation. Results: SC downregulated PDE5A expression and had no effect on other studied angiogenic factors. VEGF-A expression was increased in RPL patients compared with fertile women. Similarly, VEGF production was enhanced in RPL patients’ supernatants and SC increased the concentration of PIGF in culture supernatants. SC did not affect the expression or concentration of other studied factors, nor idNK cell activity, regardless of NO synthase blockade.
Changes in Trimethylamine-N-oxide Levels in Obese Patients following Laparoscopic Roux-en-Y Gastric Bypass or Sleeve Gastrectomy in a Korean Obesity Surgical Treatment Study (KOBESS)
Authors: Lee Soo Jin , Park Young Suk , Kim Yong-Jin , Han Sang-Uk , Hwang Geum-Sook , Han Yeyoung , Heo Yoonseok , Ha Eunyoung , Ha Tae Kyung
Published: November 2021

Trimethylamine N-oxide (TMAO), a gut microbe-dependent metabolite, has been implicated as a novel risk factor for cardiovascular events related to obesity and type 2 diabetes mellitus (T2DM). The aim of the study was to test the hypothesis if TMAO is associated with the reduction of cardiovascular disease in the Korean obese patients who underwent bariatric surgery. From a subgroup of a multicenter, nonrandomized, controlled trial, titled KOBESS, 38 obese patients, 18 with and 20 without T2DM, who underwent Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG) were investigated. Bariatric surgery is indicated for Korean patients with a body mass index (BMI) ≥ 35 kg/m2 or for Korean patients with a BMI ≥ 30 kg/m2 who have comorbidities. Serum levels of TMAO and its precursors, betaine, carnitine, and choline were measured before and six months after bariatric surgery. The levels of TMAO and its precursors did not differ between obese patients with T2DM and non-T2DM at baseline. However, TMAO increased more than twofold in patients with T2DM after RYGB surgery, but not in patients without T2DM. Choline levels were decreased by half in all patients after RYGB. In patients with T2DM who underwent SG, TMAO, betaine, and carnitine levels did not change after the surgery. Furthermore, in obese patients who underwent bariatric surgery, increased TMAO levels were associated with both T2DM and RYGB, while reduced choline levels were associated with RYGB. These associations need to be further elucidated in follow-up studies to gain further insights into the relationship between TMAO levels and bariatric surgery outcomes.
Prognostic Analysis of Patients with Acute Myocardial Infarction Undergoing Implantation of Different Stents for the First Time
Authors: Cheng Cheng-Chung , Yu Fang-Han , Ko Pi-Shao , Lin Hsiao-Ting , Lin Wei-Shiang , Cheng Shu-Meng , Su Sui-Lung
Published: November 2021

For patients with acute myocardial infarction scheduled to undergo percutaneous coronary stent implantation, in most cases a drug-eluting stent is recommended as the first choice for treatment. However, there is a lack of research on the effectiveness of bare-metal stents and drug-eluting stents on patients with different types of myocardial infarction. Our objective was to explore the effects of bare-metal stents and drug-eluting stents on patients with different types of myocardial infarction in terms of major cardiovascular incidents. This retrospective cohort study included 934 patients with myocardial infarction undergoing coronary artery stent implantation for the first time at the cardiac catheter room of the Tri-Service General Hospital in the Neihu District between 2014 and 2018. Patients’ information, including demographic data, laboratory data, cardiac echocardiography results, and angiocardiography results, was collected by reviewing medical records. Cox proportional hazards regression was used to adjust the potential confounding factors, and the adjusted data were then used to compare the correlation between different types of stents and major cardiovascular incidents in patients with ST-elevation myocardial infarction and non-ST-elevation myocardial infarction. After the confounding factors were adjusted, in patients with ST-elevation myocardial infarction receiving a drug-eluting stent compared with those receiving a bare-metal stent, it was found that the mortality risk was lower in terms of all causes of death (Adj-HR = 0.26, 95% CI = 0.14–0.48, p < 0.001) and cardiogenic death (Adj-HR = 0.20, 95% CI = 0.08–0.55, p = 0.002), the risk of non-fatal myocardial infarction was lower (Adj-HR = 0.17, 95% CI = 0.04–0.73, p = 0.017), and there was no difference in the risk of revascularization at the lesion site (Adj-HR = 0.59, 95% CI = 0.24–1.43, p = 0.243). It terms of the findings in patients with non-ST-elevation myocardial infarction, those receiving a drug-eluting stent had a lower risk of revascularization at the lesion site (Adj-HR = 0.48, 95% CI = 0.24–0.97, p = 0.04); however, there was no difference in the mortality risk in terms of all causes of death (Adj-HR = 0.71, 95% CI = 0.37–1.35, p = 0.296) or cardiogenic death (Adj-HR = 0.59, 95% CI = 0.18–1.90, p = 0.379),or in the risk of non-fatal myocardial infarction (Adj-HR = 0.27, 95% CI = 0.06–1.25, p = 0.093). Compared with bare-metal stents, drug-eluting stents provide better protection against death to receivers with ST-elevation myocardial infarction; however, this protection is decreased in receivers with non-ST-elevation myocardial infarction. It is recommended that for patients with non-ST-elevation myocardial infarction who are indicated to receive a drug-eluting stent, the clinical effectiveness of the treatment must be considered.
Clinical and Epidemiological Characteristics of 1283 Pediatric Patients with Coronavirus Disease 2019 during the First and Second Waves of the Pandemic—Results of the Pediatric Part of a Multicenter Polish Register SARSTer
Authors: Pokorska-Śpiewak Maria , Talarek Ewa , Mania Anna , Pawłowska Małgorzata , Popielska Jolanta , Zawadka Konrad , Figlerowicz Magdalena , Mazur-Melewska Katarzyna , Faltin Kamil , Ciechanowski Przemysław , Łasecka-Zadrożna Joanna , Rudnicki Józef , Hasiec Barbara , Stani Martyna , Frańczak-Chmura Paulina , Zaleska Izabela , Szenborn Leszek , Toczyłowski Kacper , Sulik Artur , Szczepańska Barbara , Pałyga-Bysiecka Ilona , Kucharek Izabela , Sybilski Adam , Sobolewska-Pilarczyk Małgorzata , Dryja Urszula , Majda-Stanisławska Ewa , Niedźwiecka Sławomira , Kuchar Ernest , Kalicki Bolesław , Gorczyca Anna , Marczyńska Magdalena
Published: November 2021

This prospective multicenter cohort study aimed to analyze the epidemiological and clinical characteristics of coronavirus disease 2019 (COVID-19) in children. The study, based on the pediatric part of the Polish SARSTer register, included 1283 children (0 to 18 years) who were diagnosed with COVID-19 between 1 March 2020 and 31 December 2020. Household contact was reported in 56% of cases, more frequently in younger children. Fever was the most common symptom (46%). The youngest children (0–5 years) more frequently presented with fever, rhinitis and diarrhea. Teenagers more often complained of headache, sore throat, anosmia/ageusia and weakness. One fifth of patients were reported to be asymptomatic. Pneumonia was diagnosed in 12% of patients, more frequently in younger children. During the second wave patients were younger than during the first wave (median age 53 vs. 102 months, p < 0.0001) and required longer hospitalization (p < 0.0001). Significantly fewer asymptomatic patients were noted and pneumonia as well as gastrointestinal symptoms were more common. The epidemiological characteristics of pediatric patients and the clinical presentation of COVID-19 are age-related. Younger children were more frequently infected by close relatives, more often suffered from pneumonia and gastrointestinal symptoms and required hospitalization. Clinical courses differed significantly during the first two waves of the pandemic.
Special Teaching Formats during the COVID-19 Pandemic—A Survey with Implications for a Crisis-Proof Education
Authors: Huth Karin Christine , von Bronk Leonard , Kollmuss Maximilian , Lindner Stefanie , Durner Jürgen , Hickel Reinhard , Draenert Miriam Esther
Published: November 2021

Modern teaching formats have not been considered necessary during the COVID-19 pandemic with uncertain acceptance by students. The study’s aim was to describe and evaluate all measures undertaken for theoretical and practical knowledge/skill transfer, which included objective structured practical examinations (OSPEs) covering a communication skills training. The students’ performance in the OSPE as well as the theoretical knowledge level were assessed, of which the latter was compared with previous terms. In conservative dentistry and periodontology (4th and 5th year courses), theoretical teaching formats were provided online and completed by a multiple-choice test. Practical education continued without patients in small groups using the phantom-head, 3D printed teeth, and objective structured practical examinations (OSPEs) including communication skills training. Formats were evaluated by a questionnaire. The organization was rated as very good/good (88.6%), besides poor Internet connection (22.8%) and Zoom® (14.2%) causing problems. Lectures with audio were best approved (1.48), followed by practical videos (1.54), live stream lectures (1.81), treatment checklists (1.81), and virtual problem-based learning (2.1). Lectures such as .pdf files without audio, articles, or scripts were rated worse (2.15–2.30). Phantom-heads were considered the best substitute for patient treatment (59.5%), while additional methodical efforts for more realistic settings led to increased appraisal. However, students performed significantly worse in the multiple-choice test compared to the previous terms (p < 0.0001) and the OSPEs revealed deficits in the students’ communication skills. In the future, permanent available lectures with audio and efforts toward realistic treatment settings in the case of suspended patient treatment will be pursued.
Long-Term Experience with Hyperthermic Chemotherapy (HIVEC) Using Mitomycin-C in Patients with Non-Muscle Invasive Bladder Cancer in Spain
Authors: Plata Ana , Guerrero-Ramos Félix , Garcia Carlos , González-Díaz Alejandro , Gonzalez-Valcárcel Ignacio , de la Morena José Manuel , Díaz-Goizueta Francisco Javier , del Álamo Julio Fernández , Gonzalo Victoria , Montero Javier , Sousa-Escandón Alejandro , León Juan , Pontones Jose Luis , Delgado Francisco , Adriazola Miguel , Pascual Ángela , Calleja Jesús , Ruano Ana , Martínez-Piñeiro Luis , Angulo Javier C.
Published: November 2021

(1) Background: Intravesical mitomycin-C (MMC) combined with hyperthermia is increasingly used in non-muscle invasive bladder cancer (NMIBC), especially in the context of a relative BCG shortage. We aim to determine real-world data on the long-term treatment outcomes of adjunct hyperthermic intravesical chemotherapy (HIVEC) with MMC and a COMBAT® bladder recirculation system (BRS); (2) Methods: A prospective observational trial was performed on patients with NMIBC treated with HIVEC using BRS in nine academic institutions in Spain between 2012–2020 (HIVEC-E). Treatment effectiveness (recurrence, progression and overall mortality) was evaluated in patients treated with HIVEC MMC 40mg in the adjuvant setting, with baseline data and a clinical follow-up, that comprise the Full Analysis Set (FAS). Safety, according to the number and severity of adverse effects (AEs), was evaluated in the safety (SAF) population, composed by patients with at least one adjunct HIVEC MMC instillation; (3) Results: The FAS population (n = 502) received a median number of 8.78 ± 3.28 (range 1–20) HIVEC MMC instillations. The median follow-up duration was 24.5 ± 16.5 (range 1–81) months. Its distribution, based on EAU risk stratification, was 297 (59.2%) for intermediate and 205 (40.8%) for high-risk. The figures for five-year recurrence-free and progression-free survival were 50.37% (53.3% for intermediate and 47.14% for high-risk) and 89.83% (94.02% for intermediate and 84.23% for high-risk), respectively. A multivariate analysis identified recurrent tumors (HR 1.83), the duration of adjuvant HIVEC therapy <4 months (HR 1.72) and that high-risk group (HR 1.47) were at an increased risk of recurrence. Independent factors of progression were high-risk (HR 3.89), recurrent tumors (HR 3.32) and the induction of HIVEC therapy without maintenance (HR 2.37). The overall survival was determined by patient age at diagnosis (HR 3.36) and the treatment duration (HR 1.82). The SAF population (n = 592) revealed 406 (68.58%) patients without AEs and 186 (31.42%) with at least one AE: 170 (28.72%) of grade 1–2 and 16 (2.7%) of grade 3–4. The most frequent AEs were dysuria (10%), pain (7.1%), urgency (5.7%), skin rash (4.9%), spasms (3.7%) and hematuria (3.6%); (4) Conclusions: HIVEC using BRS is efficacious and well tolerated. A longer treatment duration, its use in naïve patients and the intermediate-risk disease are independent determinants of success. Furthermore, a monthly maintenance of adjunct MMC HIVEC diminishes the progression rate of NMIBC.
Do Metabolically Healthy People with Obesity Have a Lower Health-Related Quality of Life? A Prospective Cohort Study in Taiwan
Authors: Lin Yi-Hsuan , Chang Hsiao-Ting , Tseng Yen-Han , Chen Harn-Shen , Chiang Shu-Chiung , Chen Tzeng-Ji , Hwang Shinn-Jang
Published: November 2021

The association between metabolically healthy obesity (MHO) and health-related quality of life (HRQOL) has not been thoroughly evaluated. This study enrolled 906 adult participants aged 35–55 years between 2009 and 2010 in Northern Taiwan; 427 participants were followed up after eight years. Normal weight, overweight, and obesity were evaluated via body mass index. Metabolic health was defined as the absence of cardiometabolic diseases and having ≤1 metabolic risk factor. HRQOL was evaluated using the 36-Item Short Form Health Survey (SF-36), Taiwan version. Generalized linear mixed-effects models were used to analyze the repeated, measured data with adjustment for important covariates. Compared with metabolically healthy normal weight individuals, participants with metabolically unhealthy normal weight and obesity had a significantly poorer physical component summary score (β (95% CI) = −2.17 (−3.38–−0.97) and −2.29 (−3.70–−0.87), respectively). There were no significant differences in physical and mental component summary scores among participants with metabolically healthy normal weight, overweight, and obesity. This study showed that metabolically healthy individuals with obesity and normal weight had similar HRQOL in physical and mental component summary scores. Maintaining metabolic health is an ongoing goal for people with obesity.
Diagnostic Work-Up of the Aortic Patient: An Integrated Approach toward the Best Therapeutic Option
Authors: Pighi Michele , Giovannini Davide , Scarsini Roberto , Piazza Nicolo
Published: November 2021

Aortic stenosis (AS) is the most common valvular heart disease. In the last decade, transcatheter aortic valve implantation (TAVI) has become the standard of care for symptomatic patients at high surgical risk. Recently, indications to TAVI have also been extended to the low surgical risk and intermediate surgical risk populations. Consequently, in this setting, some aspects acquire greater relevance: surgical risk evaluation, clinical assessment, multimodality imaging of the valve, and management of coronary artery disease. Moreover, future issues such as coronary artery re-access and valve-in-valve interventions should be considered in the valve selection process. This review aims to summarize the principal aspects of a multidimensional (multidisciplinary) and comprehensive preprocedural work-up. The Heart Team is at the center of the decision-making process of the management of aortic valve disease and bears responsibility for offering each patient a tailored approach based on an individual evaluation of technical aspects together with the risks and benefits of each modality. Considering the progressive expansion in TAVI indication and technological progress, the role of a work-up and multidisciplinary Heart Team will be even more relevant.
COVID-19 in Children with Down Syndrome: Data from the Trisomy 21 Research Society Survey
Authors: Emes David , Hüls Anke , Baumer Nicole , Dierssen Mara , Puri Shiela , Russell Lauren , Sherman Stephanie , Strydom Andre , Bargagna Stefania , Brandão Ana , Costa Alberto , Feany Patrick , Chicoine Brian , Ghosh Sujay , Rebillat Anne-Sophie , Sgandurra Giuseppina , Valentini Diletta , Rohrer Tilman , Levin Johannes , Lakhanpaul Monica ,   on behalf of the Trisomy 21 Research Society COVID-19 Initiative Study Group
Published: November 2021

Adults with Down Syndrome (DS) are at higher risk for severe outcomes of coronavirus disease 2019 (COVID-19) than the general population, but evidence is required to understand the risks for children with DS, which is necessary to inform COVID-19 shielding advice and vaccination priorities. We aimed to determine the epidemiological and clinical characteristics of COVID-19 in children with DS. Using data from an international survey obtained from a range of countries and control data from the United States, we compared the prevalence of symptoms and medical complications and risk factors for severe outcomes between DS and non-DS paediatric populations with COVID-19. Hospitalised COVID-19 patients <18 years with DS had a higher incidence of respiratory symptoms, fever, and several medical complications from COVID-19 than control patients without DS <18 years. Older age, obesity, and epilepsy were significant risk factors for hospitalisation among paediatric COVID-19 patients with DS, and age and thyroid disorder were significant risk factors for acute respiratory distress syndrome. Mortality rates were low in all paediatric COVID-19 patients (with and without DS), contrasting with previous findings in adults with DS (who exhibit higher mortality than those without DS). Children with DS are at increased risk for more severe presentations of COVID-19. Efforts should be made to ensure the comprehensive and early detection of COVID-19 in this population and to identify children with DS who present comorbidities that pose a risk for a severe course of COVID-19. Our results emphasize the importance of vaccinating children with DS as soon as they become eligible.
Factors Related to Psychological Distress during the First Stage of the COVID-19 Pandemic on the Chilean Population
Authors: Ruiz-Frutos Carlos , Delgado-García Diemen , Ortega-Moreno Mónica , Duclos-Bastías Daniel , Escobar-Gómez Dánica , García-Iglesias Juan Jesús , Gómez-Salgado Juan
Published: November 2021

The health effects of COVID-19 continue to raise doubts today. In some areas, such as mental health, these doubts have scarcely been addressed. The present study analyses the effects on psychological distress during the first phase of the pandemic in Chile. A cross-sectional descriptive study was performed by using a questionnaire validated in Spain and adapted for Chile. Between 22 April and 16 December 2020, 3227 questionnaires were collected from the 16 regions of Chile, using non-probabilistic snowball sampling. Bivariate analysis and binary logistic regression were performed. The variables that could predict psychological distress during the COVID-19 pandemic in Chile were: having a poor self-perception of health OR = 4.038, 95% CI = (2.831, 5.758); being younger than 29 OR = 2.287, 95% CI = (1.893, 2.762); having diarrhea OR = 2.093, 95% CI = (1.414, 3.098); having headache OR = 2.019, 95% CI = (1.662, 2.453); being a woman OR = 1.638, 95% CI = (1.363, 1.967); having muscle pain OR = 1.439, 95% CI = (1.114, 1.859); and having had casual contact with an infected person OR = 1.410, 95% CI = (1.138, 1.747). In Chile, with a better social, economic, cultural, and health environment compared to neighboring countries, there has been a high percentage of psychological distress. It is time to prioritize measures to safeguard the mental health of Chileans, especially focused on the most vulnerable population according to our results, i.e., young women with poorer health status.
Continuous Electrical Monitoring in Patients with Arrhythmic Myocarditis: Insights from a Referral Center
Authors: Peretto Giovanni , Mazzone Patrizio , Paglino Gabriele , Marzi Alessandra , Tsitsinakis Georgios , Rizzo Stefania , Basso Cristina , Della Bella Paolo , Sala Simone
Published: November 2021

Background. The incidence and burden of arrhythmias in myocarditis are under-reported. Objective. We aimed to assess the diagnostic yield and clinical impact of continuous arrhythmia monitoring (CAM) in patients with arrhythmic myocarditis. Methods. We enrolled consecutive adult patients (n = 104; 71% males, age 47 ± 11 year, mean LVEF 50 ± 13%) with biopsy-proven active myocarditis and de novo ventricular arrhythmias (VAs). All patients underwent prospective monitoring by both sequential 24-h Holter ECGs and CAM, including either ICD (n = 62; 60%) or loop recorder (n = 42; 40%). Results. By 3.7 ± 1.6 year follow up, 45 patients (43%) had VT, 67 (64%) NSVT and 102 (98%) premature ventricular complexes (PVC). As compared to the Holter ECG (average 9.5 exams per patient), CAM identified more patients with VA (VT: 45 vs. 4; NSVT: 64 vs. 45; both p < 0.001), more VA episodes (VT: 100 vs. 4%; NSVT: 91 vs. 12%) and earlier NSVT timing (median 6 vs. 24 months, p < 0.001). The extensive ICD implantation strategy was proven beneficial in 80% of the population. Histological signs of chronically active myocarditis (n = 73, 70%) and anteroseptal late gadolinium enhancement (n = 26, 25%) were significantly associated with the occurrence of VTs during follow up, even in the primary prevention subgroup. Conclusion. In patients with arrhythmic myocarditis, CAM allowed accurate arrhythmia detection and showed a considerable clinical impact.
The First Application of a Gd3Al2Ga3O12:Ce Single-Crystal Scintillator to Neutron Radiography
Authors: Isegawa Kazuhisa , Setoyama Daigo , Kimura Hidehiko , Shinohara Takenao
Published: November 2021

Neutron radiography is regarded as complementary to X-ray radiography in terms of transmittance through materials, but its spatial resolution is still insufficient. In order to achieve higher resolution in neutron imaging, several approaches have been adopted, such as optical magnification and event centroiding. In this paper, the authors focused on modification of the scintillator. A Gd3Al2Ga3O12:Ce single-crystal scintillator was applied to neutron radiography for the first time and a spatial resolution of 10.5 μm was achieved. The results indicate that this material can be a powerful candidate for a new neutron scintillator providing a resolution in micrometer order by optimizing the optical system and increasing the scintillator luminosity.
Bacterial Endophyte Community Dynamics in Apple (Malus domestica Borkh.) Germplasm and Their Evaluation for Scab Management Strategies
Authors: Padder Shahid A. , Mansoor Sheikh , Bhat Sajad A. , Baba Tawseef Rehman , Rather Rauoof Ahmad , Wani Saima M. , Popescu Simona Mariana , Sofi Shakeela , Aziz Malik Asif , Hefft Daniel Ingo , Alzahrani Othman M. , Noureldeen Ahmed , Darwish Hadeer
Published: November 2021

The large genetic evolution due to the sexual reproduction-mediated gene assortments and propensities has made Venturia inaequalis (causing apple scab) unique with respect to its management strategies. The resistance in apple germplasm against the scab, being controlled for by more than fifteen genes, has limited gene alteration-based investigations. Therefore, a biological approach of bacterial endophyte community dynamics was envisioned across the apple germplasm in context to the fungistatic behavior against V. inaequalis. A total of 155 colonies of bacterial endophytes were isolated from various plant parts of the apple, comprising 19 varieties, and after screening for antifungal behavior followed by morphological, ARDRA, and sequence analysis, a total of 71 isolates were selected for this study. The alpha diversity indices were seen to fluctuate greatly among the isolation samples in context to microflora with antifungal behavior. As all the isolates were screened for the presence of various metabolites and some relevant genes that directly or indirectly influence the fungistatic behavior of the isolated microflora, a huge variation among the isolated microflora was observed. The outstanding isolates showing highest percentage growth inhibition of V. inaequalis were exploited to raise a bio-formulation, which was tested against the scab prevalence in eight apple varieties under controlled growth conditions. The formulation at all the concentrations caused considerable reductions in both the disease severity and disease incidence in all the tested apple varieties. Red Delicious being most important cultivar of the northwestern Himalayas was further investigated for its biochemical behavior in formulation and the investigation revealed different levels of enzyme production, chlorophyll, and sugars against the non-inoculated control.
Bioexploration and Phylogenetic Placement of Entomopathogenic Fungi of the Genus Beauveria in Soils of Lebanon Cedar Forests
Authors: Al Khoury Charbel , Nemer Georges , Humber Richard , El-Hachem Nehme , Guillot Jacques , Chehab Racha , Noujeim Elise , El Khoury Yara , Skaff Wadih , Estephan Nathalie , Nemer Nabil
Published: November 2021

The cedar forests of Lebanon have been threatened by the outbreak caused by climate change of a web-spinning sawfly, Cephalcia tannourinensis (Hymenoptera: Pamphiliidae), which negatively impacted the survival of one of the oldest tree species on earth. In this study, we investigated the occurrence of naturally soil-inhabiting entomopathogenic fungi for their role in containing the massive outbreak of this insect. We used a combination of fungal bioexploration methods, including insect bait and selective media. Morphological features and multilocus phylogeny—based on Sanger sequencing of the transcripts encoding the translation elongation factor 1-alpha (TEF-α), RNA polymerase II second largest subunit (RBP2), and the nuclear intergenic region (Bloc) were used for species identification. The occurrence rate of entomopathogenic fungi (EPF) varied with location, soil structure, forest structure, and isolation method. From 15 soil samples positive for fungal occurrence, a total of 249 isolates was obtained from all locations using different isolation methods. The phylogenetic analysis confirmed the existence of two novel indigenous species: Beauveria tannourinensis sp. nov. and Beauveria ehdenensis sp. nov. In conclusion, the present survey was successful (1) in optimizing the isolation methods for EPF, (2) investigating the natural occurrence of Beauveria spp. in outbreak areas of C. tannourinensis, and (3) in characterizing the presence of new Beauveria species in Lebanese cedar forest soil.
The Use of Voriconazole as Primary Prophylaxis for Invasive Fungal Infections in Patients Undergoing Allogeneic Stem Cell Transplantation: A Single Center’s Experience
Authors: Atoui Ali , Omeirat Nadine , Fakhreddine Omar , El Alam Raquelle , Kanafani Zeina , Abou Dalle Iman , Bazarbachi Ali , El-Cheikh Jean , Kanj Souha S.
Published: November 2021

Background: Invasive fungal infections (IFI) following allogeneic stem cell transplant (allo-HCT) are associated with high morbidity and mortality. Primary prophylaxis using voriconazole has been shown to decrease the incidence of IFI. Methods: We conducted a retrospective analysis at the Bone Marrow Transplant (BMT) unit of the American University of Beirut including 195 patients who underwent allo-HCT for hematological malignancies and received voriconazole as primary prophylaxis for IFI. The primary endpoints were based on the incidence of IFI at day 100 and day 180, and the secondary endpoint based on fungal-free survival. Results: For the study, 195 patients who underwent allo-HCT between January 2015 and March 2021 were included. The median age at transplant was 43 years. Of the patients, 63% were male, and the majority of patients were diagnosed with acute myeloid leukemia (AML) (60%). Voriconazole was given for a median of 90 days and was interrupted in 20 patients. The majority of IFI cases were probable invasive aspergillosis (8%). The incidence of IFI including proven, probable and possible IFI was 34%. The incidence of proven and probable IFI was 5% were 8%, respectively. The incidence of proven-probable (PP-IFI) was 5.1% at day 100 and 6.6% at day 180. The majority of PP-IFI cases were invasive aspergillosis (8%). A univariate analysis of patients, transplant characteristics and IFI showed a significant correlation between the type of donor, disease status before transplant, graft-versus-host disease prophylaxis used and incidence of IFI. Only disease status post-transplant showed a significant correlation with fungal-free survival in the multivariate analysis. Conclusion: Primary prophylaxis with voriconazole in allo-HCT is associated with a low incidence of IFI. More studies are required to compare various antifungal agents in this setting.
Addition of Vegetable Oil to Improve Triterpenoids Production in Liquid Fermentation of Medicinal Fungus Antrodia cinnamomea
Authors: Meng Linghui , Luo Biaobiao , Yang Yang , Faruque Mohammad Omar , Zhang Jiuliang , Li Xiaohua , Hu Xuebo
Published: November 2021

The liquid fermentation of Antrodia cinnamomea is a promising alternative source for fungus production compared to the wildly grown fruiting body. Elicitation is a strong tool to enhance the productivity in microbial cells to obtain more compounds of interest. In this study, in order to improve the fungus growth and its terpenoids production, various vegetable oils were added in the fermentation broth of A. cinnamomea. It was found that corn oil from a group of vegetable oils exhibited the best effect on the biomass and triterpenoid content. After optimization, the initial addition of 1% (v/v) corn oil plus the inoculation of 10% (v/v) mycelia led to a maximum triterpenoid yield (532.3 mg L−1), which was increased as much as fourfold compared to the blank control. Differential transcriptome analysis demonstrated that corn oil significantly enriched several metabolic pathways including glycolysis/gluconeogenesis, propanoate metabolism and transmembrane hydrophobins. The enriched pathways interacted with deferentially expressed genes (DEGs) induced by corn oil treatment. Our research provides a potential strategy for the large production of triterpenoids by the improved fermentation of A. cinnamomea.
Alternative Engineered Soils and Seed Mixes Used for Seepage Troughs
Authors: Weiss Oliver , Minixhofer Pia , Werner Nadine , Riedenbauer Stefan , Olesko Elisabeth , Lübke Katharina , Scharf Bernhard , Pitha Ulrike
Published: November 2021

Green Infrastructure measures such as seepage troughs are an integral part of sustainable urban rainwater management. In Austria, seepage troughs are currently almost exclusively produced with a 30 cm thick active soil filter passage made of topsoil. A standard seed mix is used as vegetation, which usually consists of only three different turfgrass species. During a three-year trial, engineered soils with improved properties (increased water storage capacity, infiltration rate and pore volume) were tested for their suitability as seepage troughs compared to topsoil. In addition to the standard turf seed mix, a flowering turf seed mix (34 species) and flowering meadow seed mix (53 species) were applied. The engineered and reference soils were analyzed for infiltration rate, vitality, cover ratio and inflorescence. The results were further assessed with the evaluation chart showing quantitatively the suitability of the tested soils for rainwater management. The investigations showed that engineered soils in combination with flowering meadow seed mix lead to the best results. Therefore, this type of vegetation for seepage troughs is recommended for future applications. The reference alternatives cannot be recommended.
Object Analysis and 3D Spatial Modelling for Uniform Natural Resources in China
Authors: Ying Shen , Li Chengpeng , Chen Naibin , Jia Yizhen , Guo Renzhong , Li Lin
Published: November 2021

Natural resource management has entered a new stage in 2018 in the People’s Republic of China (China) marked by the establishment of the Ministry of Natural Resources of China (MNRPRC). More functions and responsibilities are integrated in the MNRPRC to build a uniform management system for full natural resource features in China with the aim of implementing uniform spatial planning and regulation, management, use and control, surveying, and registration for full natural resources. This paper first provides a detailed analysis regarding full natural resources with the perspectives of spatial forms and rights, restrictions, and responsibilities (RRRs); then, the modelling foundation of the “uniform” concept in natural resource registration is reconsidered. Lastly, we put forward a basic conceptual model for the uniform registration of full natural resources based on LADM (Land Administration Domain Model).
Restoration of Degraded Tropical Peatland in Indonesia: A Review
Authors: Yuwati Tri Wira , Rachmanadi Dony , Pratiwi  , Turjaman Maman , Indrajaya Yonky , Nugroho Hunggul Yudono Setio Hadi , Qirom Muhammad Abdul , Narendra Budi Hadi , Winarno Bondan , Lestari Sri , Santosa Purwanto Budi , Adi Rahardyan Nugroho , Savitri Endang , Putra Pamungkas Buana , Wahyuningtyas Reni Setyo , Prayudyaningsih Retno , Halwany Wawan , Nasrul Besri , Bastoni  , Mendham Daniel
Published: November 2021

Tropical peatlands are fragile ecosystems with an important role in conserving biodiversity, water quality and availability, preventing floods, soil intrusion, erosion and sedimentation, and providing a livelihood for people. However, due to illegal logging, fire and conversion into other land use, the peatlands in Indonesia are under serious threat. Efforts to restore Indonesia’s tropical peatlands have been accelerated by the establishment of the Peatland Restoration Agency in early 2016. The restoration action policy includes the rewetting, revegetation and revitalisation of local livelihood (known as the 3Rs). This paper summarises the regulatory, institutional and planning aspects of peatland restoration, in addition to the implementation of the 3Rs in Indonesia, including failures, success stories, and the criteria and indicators for the success of peatland restoration.
Emergent Properties of Land Systems: Nonlinear Dynamics of Scottish Farming Systems from 1867 to 2020
Authors: Aspinall Richard , Staiano Michele , Pearson Diane
Published: November 2021

Dynamics of arable and pastoral farming systems in Scotland over the period 1867–2020 are documented using time series analysis methods, including for nonlinear dynamical systems. Results show arable and pastoral farming, at a national scale, are dynamic over a range of timescales, with medium- and short-term dynamics associated with endogenous system forces and exogenous factors, respectively. Medium-term dynamics provide evidence of endogenous systems-level feedbacks between farming sectors responding to change in world and national cereal prices as an economic driver, and act to dampen impacts of exogenous shocks and events (weather, disease). Regime shifts are identified in national cereal prices. Results show change and dynamics as emergent properties of system interactions. Changes in dynamics and strength of endogenous dampening over the duration of the study are associated with dynamical changes from major governmental policy decisions that altered the boundary conditions for interdependencies of arable and pastoral farming.
Evolutionary and Conservation Genetics
Authors: Scarpa Fabio , Casu Marco , Sanna Daria
Published: November 2021

Genetic variation, evolution, and conservation are three strictly interconnected words, but none of them exist without the others, unless wanting to waive a complete and operational sense [...]