bluuebunny/crossref_metadata_2025 huggingface dataset:
IDENTIFIKASI JAMUR Aspergillus sp PADA PETIS UDANG BERDASARKAN KEMASAN DI PASAR
Authors: 97 Hidayatunnafsiyah , Hartini Supri
Published: May 2023

Banyak olahan makanan yang dibuat dari bahan ikan salah satunya petis udang. Petis mengandung karbohidrat dan kadar gula yang tinggi sehingga dapat dijadikan sebagai sumber energi untuk pertumbuhan jamur. Jenis jamur yang sering mengkontaminasi makanan adalah Aspergillus sp. Jamur ini memiliki hifa bersepta dan miselium bercabang untuk reproduksi serta menghasilkan senyawa metabolit sekunder dalam bentuk mikotoksin (aflatoksin). Salah satu cara untuk melindungi atau mengawetkan produk pangan maupun non-pangan dapat dilakukan pengemasan. Kemasan yang baik adalah kemasan yang tidak menunjukan kerusakan akibat serangan mikroba. Tujuan penelitian ini untuk mengidentifikasi jamur Aspergillus sp pada petis udang berdasarkan kemasan. Penelitian ini bersifat deskriptif dengan jumlah sampel sebanyak 6 sampel yaitu 3 sampel kemasan kantong plastik dan 3 sampel petis dalam kemasan botol plastik. Sampel dilakukan pemeriksaan mikologi secara makroskopis dan mikroskopis. Teknis analisis data menggunakan univariate. Hasil penelitian menunjukan pada kemasan kantong plastik dari 3 sampel ditemukan 67% sampel positif Aspergillus sp dan 33% sampel negatif tetapi ditemukan jamur khamir. Pada kemasan botol plastik dari 3 sampel ditemukan 67% sampel positif Aspergillus sp dan 33% negatif tetapi ditemukan jamur Penicillium sp. Disimpulkan petis udang pada kemasan kantong plastik dan botol plastik ditemukan Aspergillus sp, khamir dan Penicillium sp.
Students’ Reaction on Operating Rhetorical Strategies in Promoting Linguistic Skills and Cultural Diversity at Islamic Multicultural Classrooms
Authors: Nurhayati Dwi Astuti Wahyu
Published: May 2023

This research was motivated by some multicultural classroom students’ reactions to practicing writing argumentative text including the fact that Indonesia's adult literacy rate of 95% is not accompanied by the ability to recognize and adopt composed facts, which on average is very little. This research was formerly aimed to depict how multicultural classroom students react to deploying rhetorical strategies to write and advance their linguistic skills as well as cultural diversity The research approach adopted in this research was qualitative. The types of data sources in this study are documents in the form of various thesis manuscripts from a multicultural classroom of Masters’s Study programs at UIN SATU Tulungagung. The data collection technique applied to the main data source in the form of archives or written documents is to rely on the note-taking technique and administrating one online questionnaire survey to thirty-two students as well as attending an interview. In the thesis of multicultural classroom deployed seven strategies counting definition, comparison, cause-and-effect, problem-solution, means-end, listing, partition strategies were applied by students in argumentative text units which they compiled at various levels, both at the sentence unit level, paragraph unit, and essay unit; several facets of appealing the students experience in writing argumentative text also appeared promoting linguistic and cultural diverse in composing argumentative text counting 5 linguistics facets and 2 cultural diverse such as argumentative state/disputatiousness, probing and assortment criticism, adopting composing typical, recognized arrangement, pondering on preceding insight, instructors’ concentrating on awareness development, indigenous upbringing; experience of deploying rhetorical strategies in order to enhance linguistic skill counting conceptual and procedural knowledge to the same degree credibility, logic and emotion mainly deploying cognitive, social, search and metacognitive strategies.
The Place of Lifelong Learning in the Paidia Curriculum
Authors: Heidari Samira
Published: May 2023

Lifelong learning has become an important keyword in international research and studies because of its increasing impact on educational policies globally. One of the important goals of education systems worldwide is lifelong learning. The research method is descriptive-analytical. Data collection is in the form of extracting data from Adler's books, the second category of resources related to the Paidia curriculum. The method of data analysis was classification and interpretation, which was done simultaneously with data collection. In this descriptive-analytical study, Mortimer Adler's educational thinking has been analyzed in the context of Paideia, and the relationship between public education and continuous learning has been examined.  According to the research findings, according to Adler, the public education curriculum underpins lifelong learning, and after school education, any failure in lifelong learning constitutes a failure in public education, while continuing learning. It can improve the health of students in adulthood. On the other hand, vocational education has no place in the Paideia curriculum. If, in the light of the critique of the plan, a stronger foundation for lifelong learning can be hoped for, given the place of work and profession in the field of public education. It is recommended that in future research, researchers compare Adler's perspective on continuous learning with other thinkers. One of the most important limitations of the current research was the lack of access to schools that were designed based on the Paidia program. Another suggestion for future research is to examine the schools that have been established based on the Paidia program and study the continuous learning process in them.
Sociological Understanding of Muslim Education in Multicultural Philippines
Authors: Sali Abdul Haiy Abdul
Published: May 2023

The DepEd Order No. 41, s. 2017 catapulted the implementation of the Arabic Language and Islamic Values Education (ALIVE) Program.  However, since its inception, there is a dearth of professional literature on a coherent sociological understanding of Muslim education in the Philippines. This paper aims to provide an understanding of the implementation challenges of the ALIVE program employing sociological perspectives. The study utilized a qualitative research method anchored on Case Study Research Model. Five ALIVE implementers in Metro Manila were part of the research participants. A thematic analysis was initiated and triangulation of data was employed from relevant data sources. The analysis results showed several challenges faced by the select ALIVE schools: The intended curriculum lacks appreciation of the approaches and evaluation of student learning. The scarcity of learning spaces was evident. In teaching, most of the Asatidz or Madrasah teachers require enhancement of their pedagogical competence. The cultural variance among Muslims is still evident, especially in non-Muslim communities. The findings indicate that there is a need to create deliberate plans to achieve the intended learning outcomes of the ALIVE Program. Given the various gaps and challenges identified in this study, there is a necessity to evaluate the program at the grassroots. In conclusion, maximizing and strengthening the participation of the various stakeholders and different sectors in society is imperative in the development and sustainability of Muslim education programs in the Philippines.
Some Challenges of Geomagnetism Addressed with the Use of Ground and Satellite Observations
Authors: Soloviev A.A.
Published: May 2023

—The continuous growth of geomagnetic observations requires adequate methods for their processing and analysis. On the other hand, many studies in the field of geomagnetism require accurate and reliable measurements from both ground and space. Mathematical methods of geoinformatics can provide a solution to these problems. The article describes the progress made in the field of intellectual analysis of geomagnetic data continuously recorded by magnetic observatories and low-orbit satellites, demonstrates the results in the study of rapid variations of the Earth’s core magnetic field associated with the processes in the Earth’s deep interior, and presents the investigation of near-Earth electromagnetic dynamics. The applied value of the obtained results is also shown.
Seasonal variation in landcover estimates reveals sensitivities and opportunities for environmental models
Authors: Myers Daniel , Jones David , Oviedo-Vargas Diana , Schmit John , Ficklin Darren , Zhang Xuesong
Published: May 2023

Most readily available landuse/landcover (LULC) data are developed using growing season remote sensing images often at annual time steps. We used the Dynamic World near real-time global LULC dataset to compare how geospatial environmental models of water quality and hydrology respond to growing vs. non-growing season LULC for temperate watersheds of the eastern United States. Non-growing season LULC had more built area and less tree cover than growing season data due to seasonal impacts on classifications rather than actual LULC changes (e.g., quick construction or succession). In mixed-LULC watersheds, seasonal LULC classification inconsistencies could lead to differences in model outputs depending on the LULC season used, such as an increase in watershed nitrogen yields simulated by the Soil and Water Assessment Tool. Within reason, using separate calibration for each season may compensate for these inconsistencies, but lead to different model parameter optimizations. Our findings provide guidelines on the use of near real-time and high temporal resolution LULC in geospatial models.
Exploring Sustainable Sources for Biodiesel Production: Utilizing Fish, Chicken Waste and Aquatic Weeds to Reduce Emissions"
Authors: Tabinda Amtul Bari , Ansar Javairia , Nadeem Mahnoor , Yasir Abdullah , Rasheed Rizwan , Abdullah Maria , Rehman Sana
Published: May 2023

Biodiesel is an alternative, sustainable, eco-friendly, clean, biodegradable fuel that helps to get rid of fossil fuels that are not only depleting but also causing health issues for humans and environment. In present study, waste animal fats (fish and chicken waste) and aquatic weeds (Eichhornia crassipes, Pistia stratiotes and Lemna minor) were utilized as oil source for biodiesel production. Highest lipid content shown by fish waste oil that was 36% then followed by 33% in chicken waste oil, 16.7% in Eichhornia crassipies oil, 12.6% in Lemna minor oil and 4.11% in Pistia Stratiotes oil. Fatty acids converted into fatty acid methyl esters (biodiesel) through transesterification method. Then characterized by GCMS that confirmed the presence of highest percentages like 51% of oleic acid in Chicken waste oil biodiesel, 35.7% of palmitic acid in Fish waste oil biodiesel, 14.6% of palmitoleic acid in Eichhornia crassipies oil biodiesel, 61.7% of phthalic acid in Lemna minor oil biodiesel and 10.9% of linoleic acid in Pistia Stratiotes oil biodiesel. Physicochemical analysis of each biodiesel confirmed within the ASTM standard. Emission performance of biodiesel and petro-diesel blends showed maximum decrement in CO (26%, 22%, 24% and 23%), NO (90%, 80%, 60% and 60%), SO2 (70%, 87%, 87% and 83%)and smoke (25%, 50%, 25% and 62%) were observed in fish waste oil biodiesel, Eichhornia crassipies oil biodiesel, Pistia Stratiotes oil biodiesel and Lemna minor oil biodiesel respectively. Similarly increment in CO2 (3.75%, 3.8%, 3.72%, and 3.8%) and O2 (11.62%, 11.32%, 11.57% and 11.68%) was observed respectively because biodiesel have more oxygen content that make the complete combustion of fuel. Thus, biodiesel can adopted as an alternative fuel having less environment impact.
Bioinformatics-based identification of CCNB2 as a key gene in the progression of Chromophobe cell carcinoma of kidney
Authors: Xie Yiteng , Li Junwu , Yuan Yang , Zhang Ronggui , Zhang Yuanfeng
Published: May 2023

The present work aimed to screen biomarkers associated with Chromophobe cell carcinoma of kidney(chrcc)by bioinformatics methods as key genes to predict the prognosis of chrcc.The GSE15641 dataset was acquired from Gene Expression Omnibus(GEO) database, Totally 1153 differentially expressed genes (DEGs) were identified.thereafter, DEGs were detected to carry out Gene Ontology (GO) annotation and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis. Moreover, the protein-protein interaction (PPI) networks were constructed and visualized using Cytoscape software to identify pivotal genes,including KRAS, EGFR, EHHADH and CCNB2, were obtained, with CCNB2 being identified as the only significant core gene according to Kaplan-Meier (K-M)survival analysis.According to our results, CCNB2 expression was notably higher in The Cancer Genome Atlas(TCGA)-chrcc patients than in normal samples, and the high CCNB2 expression in cancer tissues was associated with adverse clinicopathological factors. The high CCNB2 expression group had markedly poor overall survival(OS) and progression-free interval (PFI) compared with low expression group. Meanwhile, immune infiltration analysis demonstrated a positive correlation between CCNB2 expression and Th2 cells enrichment levels in chrcc patients, and a negative correlation between CCNB2 expression and Cytotoxic cells as well as DC cells. At last, according to the Gene Set Enrichment Analysis(GSEA) enrichment results including CCNB2 gene, there was a significant difference in the classification of hepatocellular carcinoma (HCC) subclass and breast cancer (BC).In the current work, CCNB2 can be considered as a predictive molecular marker and a potential therapeutic target for chrcc.
Evidence of Zelkova carpinifolia as a potential host of Tuber uncinatum in Hyrcanian temperate forests
Authors: Azar Saeed Mirzavash , Riahi Hossein
Published: May 2023

Tuber uncinatum (Tuberaceae, Pezizales) is a commercially important ectomycorrhizal (EcM) fungal species that associate with many deciduous EcM host species belonging to the Fagales lineage. Because of its host multiplicity among deciduous species, we hypothesized that it may associate with other plant lineages including Rosids which is the most recent common ancestral of the ectomycorrhizae (MRCA-EcM) of Ulmaceae radiated preceding to Fagaceae. The host plant specimens, ascomata, and soil were collected from homogeneous Caucasian elm populationsof the Hyrcanian temperate forests of Iran. Caucasian elm species diagnosis was performed using available botanical sources. The EcM symbiosis evidence and EcMF identity were illustrated by surveying gleba, spore shape, and EcM root anatomy using SEM, light, and stereo-microscopy, followed by molecular analysis. The micrographs obtained from root examination demonstrated the Hartig net penetration among cortical and epidermal cells and the presence of mantle as the EcM sheath. This study supports the EcM symbiosis of T. uncinatum with Zelkova carpinifolia.
Modest dispersal, low densities, and little change in water clarity characterize zebra mussel populations near the southern extent of their range
Authors: Cianci-Gaskill Jacob Aaron , Thorpe Anthony P , Jones John R , North Rebecca Lee
Published: May 2023

Invasive, filter feeding zebra mussels (Dreissena polymorpha) typically cause an increase in water clarity shortly after their establishment. The purpose of this study is to evaluate whether this occurred in Midwest reservoirs, near the southern edge of their North American expansion, using a 40 + year dataset. We look for regime shifts and long-term trends in annual water clarity and compare these to the estimated zebra mussel invasion date for 7 invaded reservoirs in Missouri, USA. We also look at water clarity in 26 non-invaded, reference reservoirs to evaluate if zebra mussel impacts are being masked by changes in environmental factors. Collectively, our analyses provide a weight of evidence showing that zebra mussel establishment did not increase water clarity, likely because densities are too low to result in a noticeable impact. The highest zebra mussel density we observe is 65 mussels m− 2, an order of magnitude less than in systems where they have had a sustained impact. Low densities could be due to a combination of sublethal environmental conditions. We identified common characteristics of invaded reservoirs, including reduced particulate inorganic material and water temperatures.
Expression profile and prognostic value of the circadian clock in clear cell renal cell carcinoma
Authors: Kong Bin , Zhao Chang-Hao , Wang Xin-Yue , Liu Pan , Zang Jia-Hui , Ma Jin-Peng , Wang Lu , Xu Wan-Hai
Published: May 2023

Background A growing number of studies indicate that the circadian clock is a crucial regulator of tumorigenesis and the progression of diverse cancers. Clear cell renal cell carcinoma (ccRCC) is an extremely malignant tumor with a poor prognosis. However, the implications of circadian clock genes in ccRCC remain poorly understood.Methods We evaluated the expression pattern of circadian clock genes and their prognostic significance in ccRCC. Cluster analysis and Gene set enrichment analysis (GSEA) were used to assess the biological mechanisms of subgroups. Cox regression, GO and KEGG enrichment analysis, ESTIMATE, CIBERSORTx, and ceRNA networks were utilized to investigate the prognostic significance and underlying mechanisms of the circadian clock in ccRCC.Results We found a strong correlation between 17 circadian clock genes and ccRCC prognosis. Eight circadian clock genes were chosen to construct a new risk signature that categorized all ccRCC patients as low- or high-risk. The low-risk group had significantly greater chances of survival than the high-risk group (P < 0.001). In conjunction with clinical characteristics, the risk score was identified as an independent prognostic factor for patients with ccRCC. Immune infiltration analysis revealed that T cells CD4 memory resting, Macrophages M1, Mast cells resting, Dendritic cells resting, and Monocytes were significantly higher in the low-risk subgroup, whereas the high-risk subgroup had a greater proportion of T cells regulatory (Tregs), which was consistent with the pathways identified by ssGSEA. Finally, the ceRNA network was constructed successfully, and the significantly low expression levels of FBXL3 and HLF were verified in human RCC tissues.Conclusion Our research uncovered the crucial role of circadian clock genes in tumor immunity and identified a risk signature as a promising biomarker for predicting the prognosis of patients with ccRCC. Our findings lay the groundwork for future research focusing on the circadian clock and immune microenvironment to improve the prognosis and immunotherapy responses in ccRCC.
Rhodamine B photocatalytic activity and the synthesis of Zinc Oxide and Titanium Dioxide nanoparticles mediated by Vachellia hebeclada (Acacia hebeclada).
Authors: Mathumba Penny , Seele Palesa , Bilibana Mawethu
Published: May 2023

Rhodamine B (RhB)'s photocatalytic activity was enhanced through the green chemical synthesis of metal oxide nanoparticles (ZnO and TiO2). To produce nanoparticles, zinc nitrate hexahydrate and titanium tetra-isopropoxide precursors were hydrothermally generated using extracts from Vachellia hebeclada. These synthesised nanoparticles were then calcined at high temperatures. Variations in several parameters, including their effects on the crystal size and morphological properties of nanoparticles, were investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transforms infrared spectroscopy analysis (FTIR), and UV-vis spectroscopy. With ZnO and 26.9% with TiO2 nanoparticles, the photocatalytic efficiency of RhB degradation under ultraviolet light illumination was 56.9% within 180 minutes. The reaction rate of the ZnO pseudo-first-order kinetics was the highest (0.017 min− 1) while the reaction rate of the TiO2 nanoparticles was the slowest (0.005 min− 1). The driving force for the separation of photo-excited charge carriers is taken away by the reduced photocatalytic activity of mixed-phase TiO2 particles. ZnO nanoparticles with photocatalytic applications can be produced in an environmentally friendly manner through green nano-production. According to these findings, V. hebeclada extract-mediated synthesis of ZnO and TiO2 nanoparticles may have potential applications in the photocatalytic degradation of organic pollutants like RhB. However, additional research is required to improve the synthesis procedure and investigate the effectiveness of these nanoparticles in actual wastewater treatment applications.
Modelling Policy and Macroeconomic Shocks Transmission over the Oil-Bust Cycle in Nigeria: Evidence from the last four decades
Authors: Ihebuluche Fortune Chiugo , Afolabi Joshua , Owuru Joel , Oyolola Feyisayo , Oladepo Safiyyah , Adekunle Wasiu
Published: May 2023

Given the susceptibility of the Nigerian economy to internal and external economic shocks, it became imperative to devise strategies for cushioning the effects on sectoral productivity. This study investigated the sensitivity sectors to the oil price and policy shocks evaluated the reactions of the policy space to oil price shocks, and gauged the responsiveness of key financial indicators to shocks emanating from the fiscal and monetary policy space, using annual time-series data from 1981 to 2020 (4 Decades). The study utilised the Vector Autoregression (VAR) framework, Forecast Variance Decomposition (FVD), and Impulse Response Function (IRF). Results showed that the agricultural and industrial sectors respond more to oil price and monetary shocks than fiscal shocks, while the service sector's response to these shocks is insignificant. The result confirms the existence of forward and backward linkages, particularly between the agricultural and industrial sectors. Moreover, the study established that monetary policy has a more significant reaction to global oil price shocks than fiscal policy. While interest rate spread and exchange rate are more responsive to fiscal shocks than monetary shocks, inflation is substantially driven by monetary shocks. The study highlights relevant policy options.
JEL Classification: B23, C15, C36
The psychological effect of novel coronavirus (COVID-19) epidemic on healthcare workers: A cross-sectional study in Iran
Authors: Mosallanejad Shima , Nemati Mohammad Amin Hatami , Omidi Hossein , Moallem Masoome , Vatani Kimia , Khoshdel Ali Reza
Published: May 2023

Background:
In November 2019, news of the coronavirus outbreak broke out in Wuhan, China, with patients presenting acute respiratory syndrome symptoms. Healthcare workers are one of the groups suffering from psychological disorders in epidemics due to being present on the front. This study aimed to investigate the prevalence of psychological disorders, including depression, anxiety, insomnia, and distress, during the COVID-19 pandemic in healthcare workers in hospitals in Tehran, Iran.
Methods:
This retrospective study was conducted at the Islamic Azad University, Tehran Medical Sciences Branch. Data was collected by distributing an online questionnaire among healthcare workers (HCWs) in Tehran's hospitals from February 2020 to April 2020. Depression by Patient Health Questionnaire (PHQ)-9, anxiety by The Generalized Anxiety Disorder 7-item (GAD-7) scale, insomnia Severity Index (ISI), and distress by Impact of Event Scale–Revised (IES-R) were parameters measured in this study. Data analysis was performed using SPSS V24 software.
Results:
202 HCWs participated in this study, of whom 139 (68.1%) worked in the COVID-19 services. Bivariate correlations showed that all psychological disorders scores correlated significantly (P < 0.001). Multivariate analysis showed that a history of psychological disorders and exposure to the COVID-19 service were independently and positively correlated with depression (β = 0.22, P = 0.005 and β = 0.16, P = 0.03), anxiety (β = 0.27, P = 0.0001 and β = 0.15, P = 0.05), and insomnia (β = 0.16, P = 0.03 and β = 0.21, P = 0.004) scores. Distress score was positively correlated with a history of psychological disorder (β = 0.16, P = 0.05).
Conclusion:
This study showed that the prevalence of psychological disorders, including anxiety, insomnia, distress, and depression, in HCWs, was high. Among the factors affecting its severity were more responsibility, exposure to the patients, and a positive history of mental diseases.
WITHDRAWN: Associations between attention-deficit/hyperactivity disorder and allergic diseases: A two-sample Mendelian randomization study
Authors: Zhang Xiangyu , Zhang Runlong , Zhang Yuanfeng , Lu Tao
Published: May 2023

Background
In some observational studies, attention-deficit/hyperactivity disorder has been linked to allergic diseases, but the findings are debatable. This study aimed to determine whether attention-deficit/hyperactivity are causally related to allergic asthma, allergic rhinitis, pollen allergy, allergic urticaria and allergic conjunctivitis using the two-sample Mendelian Randomization (MR) approach.
Methods
We did a two-sample Mendelian randomization (MR) study, which chose single nucleotide polymorphisms (SNPs) that are highly associated with attention-deficit/hyperactivity disorder (ADHD) levels from the Psychiatric Genomics Consortium (PGC) on 20,183 cases and 35,191 controls as our instruments. Outcomes datasets included genome-wide association study (GWAS) meta-analysis (n = 1415804). The summary statistics of outcome data were obtained from the FinnGen datasets including allergic asthma (10877 cases and 180942 controls), allergic rhinitis (8430 cases and 298829 controls), pollen allergy (4555cases and 301734 controls), allergic urticaria (1792 cases and 299491 controls) and allergic conjunctivitis (15567 cases and 293587 controls). Inverse variance weighted, MR-Egger, weighted median, were used to estimate the causal association between ADHD and allergic diseases. Cochran's Q test were used to quantify the heterogeneity of instrumental variables. MR-Egger intercept test, leave-one-out analysis, and the funnel plot were all used in sensitivity analyses.
Results
The Mendelian randomization (MR) analysis indicated that ADHD in inverse variance weighted [odds ratio (OR) = 1.0612; 95% confidence interval (CI):1.0192–1.1049; p = 0.0039] increased the risk of allergic asthma lightly. In MR sensitivity analyses of the weighted median, a similar association was found. But no evidence for an effect of ADHD on allergic asthma risk was found in additional methods: MR-Egger (OR = 0.9592, 95% CI: 0.8384–1.0974, p = 0.5457), and weighted median (OR: =1.0341, 95% CI: 0.9785–1.0929, p = 0.2330). Also, no strong evidence for an effect of ADHD on other allergic diseases (allergic rhinitis, pollen allergy, allergic urticaria and allergic conjunctivitis) incidence was found using the inverse-variance weighted method, weighted median method and MR-Egger regression.
Conclusions
Although several studies have found a link between ADHD and allergic diseases, our findings do not support that ADHD could increase allergic diseases incidence. Randomised controlled trials or Mendelian randomization studies with larger samples are still needed to draw more precise conclusions.
Role of the Maternal Gut Microbiota in Immune Activation at the Maternal-Fetal Interface Impact on Preeclampsia
Authors: Rescigno Maria , Giugliano Silvia , Carloni Sara , Schorn Tilo
Published: May 2023

Preeclampsia is a leading cause of morbidity and mortality in pregnant women, affecting 5-8% of gestations worldwide. Several factors such as abnormalities in maternal immunity, metabolic disorders and gut dysbiosis are known to contribute to disease development.
Here, we show that dysbiosis in pregnant C57BL/6J dams increased fetal resorption, impaired placental development and altered vascularization. These changes positively correlated with increased hypoxia and reduced uterine NK cell number. Moreover, intestinal dysbiosis significantly perturbated placental carbohydrate metabolism impacting NK cell IFN-γ secretion. Of note, glucose supplementation restored placental NK cell function in vitro, suggesting that the impairment was reversible and dependent on a lower glycolytic rate.
In this work, maternal gut microbiota emerges as a key player in carbohydrate metabolism during pregnancy with a pivotal role in controlling placental immunity and pregnancy outcome. These results provide insights for the identification of metabolic biomarkers and propose the microbiota as a potentially amenable target for preventive intervention of preeclampsia.
CircDock6 promotes NAFLD by regulating IGF1R through targeting mmu-let-7 g-5p
Authors: Li Peifei , Yu Siyi , Ding Xiaoyun , Xu Lei
Published: May 2023

Circular RNAs (circRNAs) are a novel kind of noncoding RNA with a closed ring structure. An increasing number of studies have shown that it plays an important role in NAFLD. In this study, we used circDock6 to explore its role and molecular mechanism in the progression of NAFLD. qRT‒PCR was used to detect the circDock6 expression level in SD liver tissues and HFD tissues. The regulatory effects of circDock6 in NAFLD were evaluated by Oil Red “O” staining, triglyceride detection, and western blot (WB) analysis. CircDock6 was significantly upregulated in HFD liver tissues compared to SD tissues. Additionally, Oil Red “O” staining and triglyceride detection revealed that decreased expression levels of circDock6 inhibited lipid droplet formation and reduced triglyceride content. CircDock6 acts as a mmu-let-7 g-5p sponge to promote the progression of NAFLD by regulating IGF1R. Our data demonstrated that circDock6 was expressed at high levels in HFD tissues. Knockdown of circDock6 expression inhibited NAFLD progression by regulating IGF1R through targeting mmu-let-7 g-5p.
Arteriovenous fistula : Rare but serious complication of the temporomandibular joint arthroscopy
Authors: Santo Luciano Del , Nasim Omar , Leal João Victor , Ramos Thiago , Cavalcante Mateus , Villari Thiago , Sincos Igor
Published: May 2023

Background: Temporomandibular joint (TMJ) arthroscopy is a safe and minimally invasive procedure involving arthroscopic examination and operative surgery for joint arthrogenous disorders. Common complications of surgery include fluid extravasation, bleeding, and damage to the joint and associated structures. However, most of them are transient and easily controlled. Of the very rare complications is an arteriovenous fistula (AVF), which is an abnormal connection between an artery and a vein as a result of iatrogenic injury from TMJ arthroscopy.
Case presentation: We present a case of 52-year-old woman who presented with a whooshing sound, an audible bruit and a palpable thrill related left TMJ region after TMJ arthroscopy. The patient was suspected of vascular lesion and was diagnosed with an AVF involving a left transverse facial artery and superficial temporal vein by selective angiography. The patient underwent successful embolisation of the AVF under local anesthesia with intra-operative confirmation using angiography and clinical resolution of the symptoms.
Conclusion: Due to its rarity, clinicians need to be aware of its presentation and the need for a multidisciplinary approach in order to provide timely and efficient treatment. To the best of our knowledge, we report the first case of AVM secondary to TMJ arthroscopy involving transverse facial artery and superficial temporal vein.
Characterizations of a clinical Gallibacterium anatis strain from poultry in the respiratory tract：a case report
Authors: wu shihai , wei miaoyi , liu haoyang , yu chenyun , gong shimiao , si xiaohui , he zhiyuan , liu xiaoye , dong hong
Published: May 2023

Background Gallibacterium anatis, is a gram-negative coccoba-cillus that belong to the family of Pasteurellaceae. It is the historically considered an opportunistic pathogen of intensively reared poultry and domestic birds. It is the main cause of salpingitis, ovaritis and peritonitis. G. anatis infection lead egg yield and egg quality of laying hens will be reduced after infection. G. anatis has seriously affected the overall productivity of the world poultry industry and caused economic losses in the poultry industry.Case presentation Herein, we reported that this pathogen can cause obvious respiratory diseases in hens and serious harm to the reproductive tract of laying hens. The clinical symptoms of sick chickens include tracheal hemorrhage, yellow inflammatory secretion, pulmonary hemorrhage, tubal edema, yolk rupture and yolk peritonitis. We isolated and cultured the pathogenic bacteria. The characteristics of pathogenic bacteria were investigated by hemolytic activity assay, biochemical assay, and drug sensitivity test.Conclusions Based on the isolation, identification and characteristic detections of this isolations showed that the pathogen was gram-negative bacterium Gallibacterium anatis, which could ferment glucose, mannitol, inositol, and sorbitol. Catalase and oxidase test were positive. Cephalosporins and tetracyclines have good inhibitory effect on the pathogen, β- Lactam antibiotics will have strong resistance.
Top management green commitment, green process innovation and green intellectual capital as enablers of hotel environmental performance: The mediating role of green human resource management
Authors: Rehan Muhammad , Hussain Yasir , Khan Jahanzaib , Amjad Ahmad , Ali Tahir
Published: May 2023

The authors have requested that this preprint be removed from Research Square.
Robotic versus laparoscopic versus open ileoanal pouches in a busy District General Hospital setting
Authors: Patel Maitreyi S. , Aguayo Xavier , Kaul Sandeep , Banerjee Saswata , Boulton Richard , Huang Joseph , Hanson Matthew , Rajendran Nirooshun
Published: May 2023

Purpose
Ileo-anal pouch anastomosis (IPAA) is an optimal procedure for patients with chronic ulcerative colitis and familial adenomatous polyposis (FAP), for whom restoration of continuity is a priority. Due concerns of long term outcomes, there have been discussions about centralisation of this procedure. We report the evolution of the service at our trust. The aim of this study was to present our series of consecutive patients who underwent IPAA (open, laparoscopic and robotic) with respect to the surgical and functional outcomes.
Methods
Data were prospectively collected from consecutive IPAA cases from January, 2014 to March, 2023. Patient demographics, operative details, postoperative complications, functional outcomes were collected. Patients were selected by IBD specialized surgeons and reviewed by a pouch nurse. Follow-up consisted of clinical examination and assessment of pouch function.
Results
Thirty-six patients underwent IPAA over the study period. 18 underwent laparoscopic surgery, of these, two were converted to open. 6 underwent robotic, one was converted to open, making 15 open cases. The median operative time was 395minutes. Major complications (Clavien-Dindo III-IV) were seen in three patients (8.34%). Median post operative length of stay was 8 days. The 30-day re-admission rate was 30.56%, the most common cause being high stoma output. There were no mortalities.
The median Oresland Score was 6, and SIBDQ score was 51.
Conclusion
Our data suggests that with good patient selection and guidance from an experienced team, is safe and feasible with good peri-operative outcomes and acceptable pouch function in a District General Hospital setting.
Landscape use and habitat configuration effects on amphibian diversity in southern Brazil wetlands
Authors: Oro Natália , Moser Camila Fernanda , Dalzochio Marina Schmidt , de Oliveira Marcelo Zagonel , Hadi Arel , Preuss Jackson Fábio , Tozetti Alexandro Marques
Published: May 2023

In this study, we evaluated the effect of changes in natural wetlands on the amphibian diversity in spatial and temporal scales. We sampled 10 wetland sites along floodplains in southern Brazil. We classify the sites as reference or altered ponds according to the preservation degree and presence of human impact. The amphibian monitoring was conducted by calling surveys performed between 2015/2016 using an automated recording system that identified the calling male species. We identified 23 species, mainly distributed in the families Hylidae (43%) and Leptodactylidae (34.8%). The altered ponds had lower diversity and higher species dominance. Even ponds with the greatest landscape change revealed an expressive resilience concerning the amphibian species composition. However, only Boana pulchella was dominant in altered ponds and B. pulchella and Pseudopaludicola falcipes were dominant in reference ponds. The landscape changes favored the reduction of amphibian richness which was driven by the expansion of the urban area and loss of flooding areas. From 1999 to 2016 all sampled sites had their wetlands reduced is area as the surrounding urban area increased, which is an unfavorable scenario for the maintenance of biodiversity. This diagnosis reveals the combined loss of habitat and reproductive sites of anurans in subtropical wetlands.
SLC35G2 as a Prognostic Biomarker in Hepatocellular carcinoma and Its Correlation with Immunity
Authors: Meng Yanqiu , Yuan Lebing , Huang Xianbin , Li Youhua , Fu Sansan , Peng Xiaodong
Published: May 2023

Hepatocellular carcinoma (HCC) is the major cause of the worldwide cancer burden, especially in China. Solute Carrier Family 35 Member G2 (SLC35G2), a methylation-related gene, plays an essential role during tumorigenesis. However, its roles in key biological functions, the tumor microenvironment, mutations, and single-cell sequencing analysis remain unclear in HCC. This study aimed to identify the correlation between SLC35G2 and prognosis, biological roles, and immune features in HCC. The abnormal expression of SLC35G2 was associated with multiple tumor types, and there was a significant upregulation in HCC samples compared to normal tissues, which was an independent prognostic factor for predicting poor overall survival (OS) and disease-specific survival (DSS) in HCC. A nomogram based on SLC35G2, age, gender, histologic grade, and T-, N-, and M-stages was constructed, and the prognostic model performed well as shown by calibration curves for the 1-, 3-, and 5-year OS. Gene set enrichment analysis showed that SLC35G2 was closely related to tumorigenesis and immune response pathways, including Hippo-merlin, PI3K-AKT, IL-8, and IL-10 signaling pathways. In addition, SLC35G2 expression was inversely correlated with eosinophils and Th17 cells, and increased SLC35G2 expression was significantly associated with immune checkpoint molecules (GI24, CTLA4, PD-L1, B7-H3, TIM-3, and TGF-β). Furthermore, single-cell sequencing analysis showed that SLC35G2 expression was primarily localized in NK/T cells. In conclusion, SLC35G2 was identified as a new prognostic marker and had important potential implications for immunotherapy in HCC.
Epidemiology of Thoracolumbar Fractures: A Cross-Sectional Study
Authors: Junior Deoclides Lima B , Sá Brenno Franklyin A , POKORNY GABRIEL , Almeida Nivaldo S. , Silva Douglas Richard C da , Silva João Pedro , Nascimento José William Araújo do , Carneiro Geraldo de S.
Published: May 2023

Purpose: This study aims to analyze the epidemiological profile of operated patients victims of thoracolumbar fractures.
Methods: This was a retrospective cross-sectional descriptive study. The sample was composed of a total of 223 cases of thoracolumbar fractures between 2015 and 2019. Data (sex, age, site of origin, etiology of injury, Frankel scale of neurological deficit, and affected vertebral segment) were extracted from records of patients who were victims of trauma to the thoracic and lumbar spine and surgically treated in the institution.
Results: 223 patients were analyzed, of which 164 (73.5%) were male. The mean age was 35.9 ± 14.3 years. Most traumas were caused by motorcycle accidents (36.7%), followed by falls from heights (31.8%) and car accidents (17.0%). The main cause of trauma in men was motorcycle accidents (39.0%), while in women it fell from height (35.5%). The segment most affected was the thoracolumbar spine in 118 (52.9%) of the cases, followed by the thoracic segment in 78 (34.9%). 46.1% of patients admitted with Frankel E. Traffic accidents were the main causes of complete motor deficit at hospital admission (FRANKEL A).
Conclusions: Among surgically treated patients with trauma to the thoracic and/or lumbar spine, there was a predominance of motorcycle accidents followed by car accidents and men who were significantly prone to fractures. Furthermore, the thoracolumbar transition was the region most affected region; however, the injuries in the thoracic region were the ones that most exhibited the worst Frankel status.
Diabetes Mellitus Type 2 as a Risk Factor and Outcome Modifier for Cryptococcosis in HIV negative, non-transplant Patients, a Propensity Score Match Analysis
Authors: Kung Vanessa M. , Ferraz Carolina , Kennis Matthew , Franco-Paredes Carlos , Barahona Lilian Vargas , Shapiro Leland , III George R Thompson , Chastain Daniel B. , Henao-Martínez Andrés
Published: May 2023

Cryptococcosis is an opportunistic fungal infection of worldwide distribution with significant associated morbidity and mortality. HIV, organ transplantation, malignancy, cirrhosis, sarcoidosis, and immunosuppressive medications, are established risk factors for cryptococcosis. Type 2 diabetes mellitus (DM2) has been hypothesized as a risk factor and an outcome modifier for cryptococcosis. We aimed to compare outcomes among HIV-negative, non-transplant (NHNT) patients with and without DM2. We also characterized a cohort of patients with cryptococcosis and DM2 as the only identifiable risk factor. We queried a global research network to identify NHNT patients (n = 3,280). We performed a propensity score-matched (PSM) analysis comparing clinical outcomes among cryptococcosis patients with DM2 (n = 995) versus those without DM2 (n = 2,285). We also characterize adults with cryptococcosis and DM2 as the only risk factor. After PSM, NHNT patients with DM2 were more likely to develop cognitive dysfunction [9% vs. 6%, OR = 1.6; 95% CI (1.1–2.3); p = 0.01] but had similar mortality, hospitalization, ICU, and stroke risk after acquiring cryptococcosis when compared to NHNT patients without DM2. We identified 44 patients with cryptococcosis who had DM2 as the only identifiable risk factor for infection. Patients with cryptococcosis and isolated DM2 commonly had chronic kidney and end-stage renal disease. The most common anatomic site of cryptococcal infection was pulmonary. The annual incidence of cryptococcosis in patients with DM2 as their only identifiable risk factor was only 0.001%, and the prevalence was 0.002%. DM2 is associated with increased cognitive dysfunction risk in NHNT patients with cryptococcosis. It is rare for DM2 to be the only identified risk factor for developing cryptococcosis. Kidney disease, hyperglycemia, and immune dysfunction can increase risk in this setting.
Validity of BiliDx as a point-of-care bilirubin measurement device to diagnose neonatal jaundice and monitoring phototherapy at Muhimbili National Hospital, an observational study
Authors: Clemence Pascal , Moshiro Robert , Manji Karim
Published: May 2023

Background Neonatal jaundice is a condition caused by elevated levels of bilirubin in the bloodstream. Laboratory determination of serum bilirubin concentration by total serum bilirubin (TSB) test is still considered as gold standard for clinical guidance and practice. In developed countries diagnosis of neonatal jaundice is shifting towards point-of-care medical devices. So, this study aimed to determine the accuracy of the BiliDx device relative to a laboratory total serum bilirubin to diagnose and monitor jaundice among neonates admitted at Muhimbili National Hospital (MNH).Material and Methodology: This was a prospective hospital-based observational study conducted at the Neonatal Ward – MNH, Dar-es-Salaam, Tanzania from November 2022 to January 2023. A total of 181 neonates admitted at the neonatal ward with Jaundice and whose parents consented were enrolled in the study. Blood samples were collected; 2 mls of venous blood into the vacutainer bottle for routine laboratory measurement of total serum bilirubin (TSB) and 25µL blood collected into a transfer pipette tube and applied to BiliDx. STATA version 15.1 was used for data analysis.Results Out of 181 neonates, 39.2% (71/181) had birth weight between 1500g − 2499.9g, approximately 2/3rd (121/181) were preterm, 92/181 (50.8%) were males and 100/181 (55.2%) were undergoing phototherapy treatment the moment sample taken. The median bilirubin concentration was 76.9 [IQR: 54.7-129.9] mmol/l for BiliDx and 122.4 [IQR: 88.7–182.0] mmol/l for TSB. The minimum and maximum values obtained with BiliDx were, 3.4 and 427.5 mmol/l respectively, compared with 10.7 and 533.1 mmol/l using TSB. A linear relationship and correlation coefficient of 0.8408 (p = 0.000) between BiliDx and TSB was found. The regression analysis showed the presence of constant error [coefficient of BiliDx/slope = 0.91, 95% CI (0.82–0.99), p = 0.000] and random error exclusively [coefficient of constant/y-intercept = 48.52, 95%CI (37.70-59.34), p = 0.000]. The Bland–Altman plot showed an acceptable mean difference of 39.1mmol/l, limits of agreement of -48.3mmol/l to 126.4mmol/l and 179 points (179/181 = 98.9%) lying inside the limits of agreement.Conclusion The results support the use of BiliDx for rapid and accurate testing of elevated levels of bilirubin in the bloodstream among neonates since 98.9% of the differences between BiliDx and standard laboratory TSB lie between the lines of agreements.
Climate change impacts on ocean light in Arctic ecosystems beyond sea ice
Authors: Kristiansen Trond , Varpe Øystein , Selig Elizabeth , Laurel Benjamin , Sydeman William , Hegglin Michaela , Wallhead Phil
Published: May 2023

Climate change will drive sea ice loss, resulting in increased light availability across polar marine ecosystems, but the consequences of these changes are largely unknown. We quantify how future conditions for sea ice and snow, storm-driven waves, clouds, ozone, and chlorophyll will affect the seasonal absorption and reflection of light in Arctic seas. Using CMIP6 inputs and a spectral radiative transfer model, we predict a 20-50% increase in visible light by 2100 in the Northern Bering/Chukchi and Barents Seas ecosystems. For cold-water fish species, warmer summers and reduced phytoplankton will decrease survival in summer, while asynchrony in prey and light availability will decrease survival in fall. Warmer-water species will be less impacted. Coupled with ocean warming, increased light availability will accelerate changes in Arctic ecosystems, compromising the growth and survival of Arctic species in transitional zones and facilitating the northward expansion of boreal species.
Microbial iron reduction under oxic conditions: when microfluidics meets geochemistry
Authors: Ceriotti Giulia , Berg Jasmine , Borisov Sergey , Santos Alice Bosco
Published: May 2023

Iron (Fe) reduction is one of Earth's most ancient microbial metabolisms, but after atmosphere-ocean oxygenation, this anaerobic process was relegated to niche anoxic environments below the water and soil surface. However, new technologies to monitor redox processes at micrometer scales relevant to microbial cells have the potential to reveal how oxygen (O2) concentrations control the distribution of aerobic and anaerobic metabolisms. To explore the impact of varying O2levels on microbial Fe reduction, we cultivated a facultative Fe-reducing bacterium in a novel microfluidic reactor integrated with transparent planar O2sensors. Contrary to expectations, microbial growth induced Fe(III)-oxide (ferrihydrite) reduction under fully oxygenated conditions, without the formation of O2-depleted micro-sites. Our observations fundamentally change our understanding of Fe cycling in ancient and contemporary environments, from our interpretation of Fe mineralogy in the rock record to heavy metal and nutrient mobility in the modern subsurface.