bluuebunny/crossref_metadata_2025 huggingface dataset:
Site-Specific, Critical Threshold Barefoot Peak Plantar Pressure Associated with Diabetic Foot Ulcer History: A Novel Approach to Determine DFU Risk in the Clinical Setting
Authors: Abbott Caroline A. , Chatwin Katie E. , Rajbhandari Satyan M. , John Kanwal M. , Pabbineedi Sushma , Bowling Frank L. , Boulton Andrew J. M. , Reeves Neil D.
Published: January 2022

Background and Objectives: Barefoot peak plantar pressures (PPPs) are elevated in diabetes patients with neuropathic foot ulcer (DFU) history; however, there is limited reported evidence for a causative link between high barefoot PPP and DFU risk. We aimed to determine, using a simple mat-based methodology, the site-specific, barefoot PPP critical threshold that will identify a plantar site with a previous DFU. Materials and Methods: In a cross-sectional study, barefoot, site-specific PPPs were measured with normal gait for patients with DFU history (n = 21) and healthy controls (n = 12), using a validated carbon footprint system. For each participant, PPP was recorded at twelve distinct plantar sites (1st–5th toes, 1st–5th metatarsal heads (MTHs), midfoot and heel), per right and left foot, resulting in the analysis of n = 504 distinct plantar sites in the diabetes group, and n = 288 sites in the control group. Receiver operator characteristic curve analysis determined the optimal critical threshold for sites with DFU history. Results: Median PPPs for the groups were: diabetes sites with DFU history (n = 32) = 5.0 (3.25–7.5) kg/cm2, diabetes sites without DFU history (n = 472) = 3.25 (2.0–5.0) kg/cm2, control sites (n = 288) = 2.0 (2.0–3.25) kg/cm2; (p < 0.0001). Diabetes sites with elevated PPP (>6 kg/cm2) were six times more likely to have had DFU than diabetes sites with PPP ≤ 6 kg/cm2 (OR = 6.4 (2.8–14.6, 95% CI), p < 0.0001). PPP > 4.1 kg/cm2 was determined as the optimal critical threshold for identifying DFU at a specific plantar site, with sensitivity/specificity = 100%/79% at midfoot; 80%/65% at 5th metatarsal head; 73%/62% at combined midfoot/metatarsal head areas. Conclusions: We have demonstrated, for the first time, a strong, site-specific relationship between elevated barefoot PPP and previous DFU. We have determined a critical, highly-sensitive, barefoot PPP threshold value of >4.1 kg/cm2, which may be easily used to identify sites of previous DFU occurrence and, therefore, increased risk of re-ulceration. This site-specific approach may have implications for how high PPPs should be investigated in future trials.
Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
Authors: Kis Andreea , Macasoi Ioana , Paul Corina , Radulescu Matilda , Buzatu Roxana , Watz Claudia , Cheveresan Adelina , Berceanu Delia , Pinzaru Iulia , Dinu Stefania , Manea Aniko , Poenaru Marioara , Borza Claudia , Dehelean Cristina
Published: January 2022

Background Objectives: The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) are two basic chemotherapeutics used in cancer practice, but notwithstanding despite many years of use, the mechanisms by which the multitude of side-effects occur are not yet fully understood. Thus, the present study focused on the in vitro and in ovo evaluation of the associated toxic mechanisms on keratinocytes, keys cells in the wound healing process. Materials and Methods: The two chemotherapeutics were tested in eight different concentrations to evaluate keratinocytes viability, the anti-migratory effect, and the influence on the expression of markers involved in the production of cell apoptosis. In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM). Results: The results revealed that Met induced decreased cell viability as well as increased expression of pro-apoptotic genes. In the vascular plexus of the chorioallantoic membrane, Met caused vascular irritation accompanied by capillary hemorrhage and vascular stasis. Conclusions: Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques.
In Vitro and In Vivo Feasibility Study for a Portable VV-ECMO and ECCO2R System
Authors: Strudthoff Lasse J. , Lüken Hannah , Jansen Sebastian V. , Petran Jan , Schlanstein Peter C. , Schraven Lotte , Schürmann Benjamin J. , Steuer Niklas B. , Wagner Georg , Schmitz-Rode Thomas , Steinseifer Ulrich , Arens Jutta , Kopp Rüdger
Published: January 2022

Extracorporeal membrane oxygenation (ECMO) is an established rescue therapy for patients with chronic respiratory failure waiting for lung transplantation (LTx). The therapy inherent immobilization may result in fatigue, consecutive deteriorated prognosis, and even lost eligibility for transplantation. We conducted a feasibility study on a novel system designed for the deployment of a portable ECMO device, enabling the physical exercise of awake patients prior to LTx. The system comprises a novel oxygenator with a directly connected blood pump, a double-lumen cannula, gas blender and supply, as well as control and energy management. In vitro experiments included tests regarding performance, efficiency, and blood damage. A reduced system was tested in vivo for feasibility using a novel large animal model. Six anesthetized pigs were first positioned in supine position, followed by a 45° angle, simulating an upright position of the patients. We monitored performance and vital parameters. All in vitro experiments showed good performance for the respective subsystems and the integrated system. The acute in vivo trials of 8 h duration confirmed the results. The novel portable ECMO-system enables adequate oxygenation and decarboxylation sufficient for, e.g., the physical exercise of designated LTx-recipients. These results are promising and suggest further preclinical studies on safety and efficacy to facilitate translation into clinical application.
Designing a Functional CNT+PB@MXene-Coated Separator for High-Capacity and Long-Life Lithium–Sulfur Batteries
Authors: Wang Guiling , Li Jiaojiao , Du Zhiling , Ma Zhipeng , Shao Guangjie
Published: January 2022

Separators, as indispensable parts of LSBs (lithium–sulfur batteries), play a cucial role in inhibiting dendrite growth and suppressing the shuttle of lithium polysulfide (LiPSs). Herein, we prepared a functional carbon nanotube (CNT) and Fe-based Prussian blue (PB)@MXene/polypropylene (PP) composite separator using a facile vacuum filtration approach. The CNTs and MXene nanosheets are excellent electronic conductors that can enhance the composite separator electrical conductivity, while Fe-based Prussian blue with a rich pore structure can effectively suppress the migration by providing physical space to anchor soluble LiPSs and retain it as cathode active material. Additionally, MXene nanosheets can be well attached to Fe-based Prussian blue by an electrostatic interaction and contribute to the physical barriers that inhibit the shuttle of long-chain soluble Li2Sn (4 ≤ n ≤ 8). When used as a lithium–sulfur (Li–S) cell membrane with a functional coating layer of CNT+PB@MXene facing the cathode side, the batteries reveal a high initial discharge capacity (1042.6 mAh g−1 at 0.2 C), outstanding rate capability (90% retention of capacity at 1.0 C) and high reversible capacity (674.1 mAh g−1 after 200 cycles at 1.0 V). Of note, separator modification is a feasible method to improve the electrochemical performance of LSBs.
Intensification of Dry Reforming of Methane on Membrane Catalyst: Confirmation and Development of the Hypothesis
Authors: Gavrilova Natalia , Gubin Sergey , Myachina Maria , Sapunov Valentin , Skudin Valery
Published: January 2022

This article presents an analysis of kinetic studies of dry methane reforming (DRM) in a reactor with a membrane catalyst (RMC) in the modes of a contactor with “diffusion” and “forced” mass transfer. Comparison of the specific rate constants of the methane dissociation reaction in membrane and traditional reactors confirmed the phenomenon of intensification of dry methane reforming in a membrane catalyst (MC). It has been experimentally established that during DRM, a temperature gradient arises in the channels of the pore structure of the membrane catalyst, characterized by a decrease in temperature towards the inner volume of the MC, and initiates the phenomenon of thermal slip. The features of this phenomenon are highlighted and must be considered in the analysis of kinetic data. The main provisions of the hypothesis explaining the effect of intensification by the occurrence of thermal slip in the channels of the pore structure of the MC are formulated. The proposed hypothesis, based on thermal slip, explains the difference in rate constants of traditional and membrane catalysts, and substantiates the phenomenological scheme of DRM stages in a reactor with a membrane catalyst.
Electrospun ZnSnO3/ZnO Composite Nanofibers and Its Ethanol-Sensitive Properties
Authors: Dong Songtao , Jin Xiaoyun , Wei Junlin , Wu Hongyan
Published: January 2022

In this work, a novel heterojunction based on ZnSnO3/ZnO nanofibers was prepared by a simple electrospinning method. The crystal, structural, and surface compositional properties of ZnSnO3 and ZnSnO3/ZnO composite nanofibers were investigated by X-ray diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectrometer (XPS), and Brunauer–Emmett–Teller (BET). Compared to pure ZnSnO3 nanofibers, the ZnSnO3/ZnO heterostructure nanofibers had high sensitivity and selectivity response with the fast response toward ethanol gas at low operational temperature. The sensing response of the sensor based on ZnSnO3/ZnO composite nanofibers was 19.6 toward 50 ppm ethanol gas at 225 °C, which was about 1.5 times superior to that of pure ZnSnO3 nanofibers. It can be owed mainly to the oxygen vacancies and the synergistic effect between ZnSnO3 and ZnO.
Investigation of Co-Cr-Fe-Mn-Ni Non-Equiatomic High-Entropy Alloy Fabricated by Wire Arc Additive Manufacturing
Authors: Osintsev Kirill , Konovalov Sergey , Zaguliaev Dmitrii , Ivanov Yurii , Gromov Victor , Panchenko Irina
Published: January 2022

Fabrication of high-entropy alloys (HEAs) is a crucial area of interest for materials scientists since these metallic materials may have many practical uses. Wire arc additive manufacturing (WAAM), unlike other additive technologies, has tangible benefits for making large-sized components, but manufacturing the wire from HEAs is still very limited. Recent studies suggested tackling this problem using a combined cable composed of wires consisting of pure elements as feeding material. However, not all compositions of HEAs can be obtained by the pure elements’ wires because the number of them is limited. This study aims to examine phase composition, chemical elements distribution, microstructure, and mechanical properties of a Co-Cr-Fe-Mn-Ni HEA, which was not previously obtained by the WAAM. The cable-type wire used in this study is composed of two wires which consist of Cr, Fe, Mn, and Ni, and one pure Co wire. The phase composition, chemical elements distribution, microstructure, and mechanical properties were investigated. The prepared high-entropy alloy has non-equiatomic chemical composition with a single-phase FCC crystal structure with homogeneously distributed elements inside the grains. The microstructure examinations showed dendrite structure which is typical for WAAM processes. The compressive yield strength of the alloy is ~279 MPa, the ultimate compressive strength is ~1689 MPa, the elongation is 63%, and the microhardness is ~150 HV, which was found to be similar to the previously fabricated Co-Cr-Fe-Mn-Ni alloys by other methods. Fracture analysis confirmed the ductile behavior of deformation by the presence of dimples.
Recrystallization and Grain Growth of AISI 904L Super-Austenitic Stainless Steel: A Multivariate Regression Approach
Authors: Stornelli Giulia , Gaggiotti Matteo , Mancini Silvia , Napoli Giuseppe , Rocchi Claudia , Tirasso Chiara , Di Schino Andrea
Published: January 2022

AISI 904L is a super-austenitic stainless steel that is remarkable for its mechanical properties and high corrosion resistance, which strictly depend on its chemical composition and microstructural features. The recrystallization process and grain growth phenomena play key roles in achieving high levels of material quality, as often requested by customers for specific applications. In this paper, the evolution of the microstructure and hardness values after cold rolling and subsequent annealing is reported, with the aim of optimizing the thermomechanical treatment conditions and improving the efficiency of the production process. The investigation was focused on three different cold reduction ratios (50%, 70% and 80%), while combining different annealing temperatures (950, 1050 and 1150 °C) and soaking times (in the range of 20–180 s. The test results were organized using a data analysis and statistical tool, which was able to show the correlation between the different variables and the impacts of these on recrystallization and grain growth processes. For low treatment temperatures, the tested soaking times led to partial recrystallization, making this condition industrially unattractive. Instead, for the higher temperature, full recrystallization was achieved over a short time (20–40 s), depending on the reduction ratio. Regarding the grain growth behavior, it was found to be independent of the reduction ratio; for each treatment temperature, the grain growth showed a linear trend as a function of the soaking time only. Moreover, the static recrystallization kinetics were analyzed using a statistical analysis software program that was able to provide evidence indicating the most and least influential parameters in the process. In particular, taking into consideration the hardness values as output data, the temperature and soaking time were revealed to have major effects as compared with the reduction ratio, which was excluded from the statistical analysis. The prediction approach allowed us to formulate a regression equation in order to correlate the response and terms. Moreover, a response optimizer was used to predict the best solution to get as close as possible to the hardness target required by the market.
Influence of a Thermo-Mechanical Treatment on the Fatigue Lifetime and Crack Initiation Behavior of a Quenched and Tempered Steel
Authors: Khayatzadeh Amin , Sippel Jan , Guth Stefan , Lang Karl-Heinz , Kerscher Eberhard
Published: January 2022

A thermo-mechanical treatment (TMT) at the temperature of maximum dynamic strain aging has been optimized and performed on quenched and tempered steel SAE4140H (German designation: 42CrMo4) in order to improve the fatigue limit in the high cycle fatigue (HCF) and and very high cycle fatigue (VHCF) regimes. Fatigue tests, with ultimate cycle numbers of 107 and 109, have shown that the TMT can increase both the fatigue lifetime and the fatigue limit in the HCF and VHCF regimes. The increased stress intensity factors of the critical inclusions after the TMT indicate that the effect can be attributed to a stabilized microstructure around critical crack-initiating inclusions through the locking of edge dislocations by carbon atoms during the TMT.
The Effect of Co-Encapsulated GO-Cu Nanofillers on Mechanical Properties, Cell Response, and Antibacterial Activities of Mg-Zn Composite
Authors: Saberi Abbas , Bakhsheshi-Rad Hamid Reza , Ismail Ahmad Fauzi , Sharif Safian , Razzaghi Mahmood , Ramakrishna Seeram , Berto Filippo
Published: January 2022

Magnesium-based composites have recently been studied as biodegradable materials for preparing orthopedic implants. In this article, the graphene oxide (GO) and GO-Cu nanosystem has been homogenously dispersed as a reinforcement in the matrix of Mg-Zn (MZ) alloy using the semi powder metallurgy (SPM) method, and subsequently, the composite has been successfully manufactured using the spark plasma sintering (SPS) process. GO and GO-Cu reinforced composite displayed a higher compressive strength (~55%) than the unreinforced Mg-Zn sample. GO and GO-Cu dual nanofillers presented a synergistic effect on enhancing the effectiveness of load transfer and crack deflection in the Mg-based matrix. Besides, the GO-Cu dual nanofillers displayed a synergistic influence on antibacterial activity through combining the capturing influences of GO nanosheets with the killing influences of Cu. However, electrochemical and in-vitro immersion evaluation showed that Cu-GO reinforcement had a slightly negative effect on the corrosion behavior of the Mg-Zn sample, but the incorporation of GO enhanced corrosion resistance of the composite. Moreover, MZ/GO and MZ/GO-Cu nanocomposites showed acceptable cytotoxicity to MG-63 cells and revealed a high potential for use as an orthopedic implant material. Based on the research results, MZ/GO-Cu nanocomposite could be used in bone tissue engineering applications.
The Crowded Uterine Horn Mouse Model for Examining Postnatal Metabolic Consequences of Intrauterine Growth Restriction vs. Macrosomia in Siblings
Authors: Taylor Julia A. , Coe Benjamin L. , Shioda Toshi , vom Saal Frederick S.
Published: January 2022

Differential placental blood flow and nutrient transport can lead to both intrauterine growth restriction (IUGR) and macrosomia. Both conditions can lead to adult obesity and other conditions clustered as metabolic syndrome. We previously showed that pregnant hemi-ovariectomized mice have a crowded uterine horn, resulting in siblings whose birth weights differ by over 100% due to differential blood flow based on uterine position. We used this crowded uterus model to compare IUGR and macrosomic male mice and also identified IUGR males with rapid (IUGR-R) and low (IUGR-L) postweaning weight gain. At week 12 IUGR-R males were heavier than IUGR-L males and did not differ from macrosomic males. Rapid growth in IUGR-R males led to glucose intolerance compared to IUGR-L males and down-regulation of adipocyte signaling pathways for fat digestion and absorption and type II diabetes. Macrosomia led to increased fat mass and altered adipocyte size distribution compared to IUGR males, and down-regulation of signaling pathways for carbohydrate and fat digestion and absorption relative to IUGR-R. Clustering analysis of gonadal fat transcriptomes indicated more similarities than differences between IUGR-R and macrosomic males compared to IUGR-L males. Our findings suggest two pathways to adult metabolic disease: macrosomia and IUGR with rapid postweaning growth rate.
Alterations of the Intestinal Permeability are Reflected by Changes in the Urine Metabolome of Young Autistic Children: Preliminary Results
Authors: Piras Cristina , Mussap Michele , Noto Antonio , De Giacomo Andrea , Cristofori Fernanda , Spada Martina , Fanos Vassilios , Atzori Luigi , Francavilla Ruggiero
Published: January 2022

Several metabolomics-based studies have provided evidence that autistic subjects might share metabolic abnormalities with gut microbiota dysbiosis and alterations in gut mucosal permeability. Our aims were to explore the most relevant metabolic perturbations in a group of autistic children, compared with their healthy siblings, and to investigate whether the increased intestinal permeability may be mirrored by specific metabolic perturbations. We enrolled 13 autistic children and 14 unaffected siblings aged 2–12 years; the evaluation of the intestinal permeability was estimated by the lactulose:mannitol test. The urine metabolome was investigated by proton nuclear magnetic resonance (1H-NMR) spectroscopy. The lactulose:mannitol test unveiled two autistic children with altered intestinal permeability. Nine metabolites significantly discriminated the urine metabolome of autistic children from that of their unaffected siblings; however, in the autistic children with increased permeability, four additional metabolites—namely, fucose, phenylacetylglycine, nicotinurate, and 1-methyl-nicotinamide, strongly discriminated their urine metabolome from that of the remaining autistic children. Our preliminary data suggest the presence of a specific urine metabolic profile associated with the increase in intestinal permeability.
A Novel Updated Full-Discretization Method for Prediction of Milling Stability
Authors: Ma Junjin , Li Yunfei , Zhang Dinghua , Zhao Bo , Wang Geng , Pang Xiaoyan
Published: January 2022

This paper presents an updated full-discretization method for milling stability prediction based on cubic spline interpolation. First, the mathematical model of the time-delay milling system considering regenerative chatter is represented by a dynamic delay differential equation. Then, in a single tooth passing period, the time is divided into a finite time intervals, the state item and the time-delay item are approximated in each time interval by cubic spline interpolation and third-order Newton interpolation, respectively. Afterward, a transition matrix is constructed to represent the transfer relationship of the teeth in a period. Finally, based on Floquet theory, the milling stability lobes can be obtained. Meanwhile, in order to improve computational efficiency, an optimized method is proposed based on the traditional algorithm and the proposed method has high precision without losing high efficiency. Finally, several milling experiments are conducted to verify the accuracy of the proposed method, and the results show that the predicted results agree well with the experimental results.
Microelectromechanical Systems (MEMS) for Biomedical Applications
Authors: Chircov Cristina , Grumezescu Alexandru Mihai
Published: January 2022

The significant advancements within the electronics miniaturization field have shifted the scientific interest towards a new class of precision devices, namely microelectromechanical systems (MEMS). Specifically, MEMS refers to microscaled precision devices generally produced through micromachining techniques that combine mechanical and electrical components for fulfilling tasks normally carried out by macroscopic systems. Although their presence is found throughout all the aspects of daily life, recent years have witnessed countless research works involving the application of MEMS within the biomedical field, especially in drug synthesis and delivery, microsurgery, microtherapy, diagnostics and prevention, artificial organs, genome synthesis and sequencing, and cell manipulation and characterization. Their tremendous potential resides in the advantages offered by their reduced size, including ease of integration, lightweight, low power consumption, high resonance frequency, the possibility of integration with electrical or electronic circuits, reduced fabrication costs due to high mass production, and high accuracy, sensitivity, and throughput. In this context, this paper aims to provide an overview of MEMS technology by describing the main materials and fabrication techniques for manufacturing purposes and their most common biomedical applications, which have evolved in the past years.
A Review of Microfluidic Devices for Rheological Characterisation
Authors: Del Giudice Francesco
Published: January 2022

The rheological characterisation of liquids finds application in several fields ranging from industrial production to the medical practice. Conventional rheometers are the gold standard for the rheological characterisation; however, they are affected by several limitations, including high costs, large volumes required and difficult integration to other systems. By contrast, microfluidic devices emerged as inexpensive platforms, requiring a little sample to operate and fashioning a very easy integration into other systems. Such advantages have prompted the development of microfluidic devices to measure rheological properties such as viscosity and longest relaxation time, using a finger-prick of volumes. This review highlights some of the microfluidic platforms introduced so far, describing their advantages and limitations, while also offering some prospective for future works.
Breakdown Behavior of Metal Contact Positions in GaN HEMT with Nitrogen-Implanted Gate Using TCAD Simulation
Authors: Sheu Gene , Song Yu-Lin , Mogarala Ramyasri , Susmitha Dupati , Issac Kutagulla
Published: January 2022

In this study, the breakdown behavior of a calibrated depletion mode AlGaN/GaN transistor with a nitrogen-implanted gate region was simulated and analyzed using Sentaurus TCAD simulation, with particular emphasis on the metal contact design rule for a GaN-based high-electron-mobility transistor (HEMT) device with a variety of 2DEG concentrations grown on a silicon substrate. The breakdown behaviors for different source/drain contact schemes were investigated using Sentaurus simulation. The metal contact positions within the source and drain exhibited different piezoelectric effects and induced additional polarization charges for the 2DEG (two-dimensional electron gas). Due to the variation of source/drain contact schemes, electron density has changed the way to increase the electric field distribution, which in turn increased the breakdown voltage. The electric field distribution and 2DEG profiles were simulated to demonstrate that the piezoelectric effects at different metal contact positions considerably influence the breakdown voltage at different distances between drain metal contacts. When the contact position was far away from the AlGaN/GaN, the breakdown voltage of the nitrogen-implanted gated device decreased by 41% because of the relatively low electron density and weak induced piezoelectric effect. This reduction is significant for a 20 μm source-drain length. The minimum critical field used for the breakdown simulation was 4 MV/cm. The simulated AlGaN/GaN device exhibits different breakdown behaviors at different metal contact positions in the drain.
Kingella kingae and Viral Infections
Authors: Basmaci Romain , Bidet Philippe , Bonacorsi Stéphane
Published: January 2022

Kingella kingae (K. kingae) is an oropharyngeal commensal agent of toddlers and the primary cause of osteoarticular infections in 6–23-month-old children. Knowing that the oropharynx of young children is the reservoir and the portal of entry of K. kingae, these results suggested that a viral infection may promote K. kingae infection. In this narrative review, we report the current knowledge of the concomitance between K. kingae and viral infections. This hypothesis was first suggested because some authors described that symptoms of viral infections were frequently concomitant with K. kingae infection. Second, specific viral syndromes, such as hand, foot and mouth disease or stomatitis, have been described in children experiencing a K. kingae infection. Moreover, some clusters of K. kingae infection occurring in daycare centers were preceded by viral outbreaks. Third, the major viruses identified in patients during K. kingae infection were human rhinovirus or coxsackievirus, which both belong to the Picornaviridae family and are known to facilitate bacterial infections. Finally, a temporal association was observed between human rhinovirus circulation and K. kingae infection. Although highly probable, the role of viral infection in the K. kingae pathophysiology remains unclear and is based on case description or temporal association. Molecular studies are needed.
Comparative Genomic Analyses of the Genus Nesterenkonia Unravels the Genomic Adaptation to Polar Extreme Environments
Authors: Dai Daoxin , Lu Huibin , Xing Peng , Wu Qinglong
Published: January 2022

The members of the Nesterenkonia genus have been isolated from various habitats, like saline soil, salt lake, sponge-associated and the human gut, some of which are even located in polar areas. To identify their stress resistance mechanisms and draw a genomic profile across this genus, we isolated four Nesterenkonia strains from the lakes in the Tibetan Plateau, referred to as the third pole, and compared them with all other 30 high-quality Nesterenkonia genomes that are deposited in NCBI. The Heaps’ law model estimated that the pan-genome of this genus is open and the number of core, shell, cloud, and singleton genes were 993 (6.61%), 2782 (18.52%), 4117 (27.40%), and 7132 (47.47%), respectively. Phylogenomic and ANI/AAI analysis indicated that all genomes can be divided into three main clades, named NES-1, NES-2, and NES-3. The strains isolated from lakes in the Tibetan Plateau were clustered with four strains from different sources in the Antarctic and formed a subclade within NES-2, described as NES-AT. Genome features of this subclade, including GC (guanine + cytosine) content, tRNA number, carbon/nitrogen atoms per residue side chain (C/N-ARSC), and amino acid composition, in NES-AT individuals were significantly different from other strains, indicating genomic adaptation to cold, nutrient-limited, osmotic, and ultraviolet conditions in polar areas. Functional analysis revealed the enrichment of specific genes involved in bacteriorhodopsin synthesis, biofilm formation, and more diverse nutrient substance metabolism genes in the NES-AT clade, suggesting potential adaptation strategies for energy metabolism in polar environments. This study provides a comprehensive profile of the genomic features of the Nesterenkonia genus and reveals the possible mechanism for the survival of Nesterenkonia isolates in polar areas.
Diversity and Phylogeny of Novel Cord-Forming Fungi from Borneo
Authors: Foster Rachel , Hartikainen Hanna , Hall Andie , Bass David
Published: January 2022

Cord-forming (CF) fungi are found worldwide; however, tropical CF fungi are poorly documented. They play an essential role in forest ecosystems by interconnecting nutrient resources and aiding in the decomposition of plant matter and woody litter. CF fungi samples were collected from two forest conservation sites in the Sabah region of Malaysian Borneo. Sequencing and phylogenetic analysis of the ribosomal rRNA gene array 18S to 28S region from cords collected placed all of the collected specimens in Agaricomycetes (Basidiomycetes), specifically within the orders Trechisporales, Phallales, Hymenochaetales, Polyporales, and Agaricales. Comparison of the cord-derived sequences against GenBank and UNITE sequence databases, as well as phylogenetic analyses, revealed they were all novel sequences types. Many of these novel lineages were found to be closely related to other basidiomycetes commonly found in tropical forests, suggesting a large undiscovered tropical fungal diversity in Borneo that has been detected independently of sampling fruiting bodies. We show how these sequence types relate to the morphologies of the cords from which they were sampled. We also highlight how rapid, small-scale sampling can be a useful tool as an easy and relatively unbiased way of collecting data on cord-forming fungi in difficult-to-access, complex forest environments, independently of locating and sampling sporophores.
Therapy and Prevention for Human Toxocariasis
Authors: Magnaval Jean-François , Bouhsira Emilie , Fillaux Judith
Published: January 2022

For the last four decades, knowledge about human toxocariasis with regard to its epidemiology, pathophysiology, clinical spectrum, and imaging or laboratory diagnosis has substantially progressed. Knowledge about specific therapy with anthelmintics has lagged behind. To date, only four drugs are registered for human use, and their efficacy has rarely been assessed in prospective controlled trials. It is likely that the repurposing of potent anthelmintics from veterinary medicine will improve this situation. Due to its wide availability and a lack of major side effects during short regimens, albendazole has become the drug of choice. However, its efficacy should be more precisely assessed. The role of anthelmintics in the treatment of neurological or ocular toxocariasis remains to be clarified. Prophylactic measures in humans or companion animals are efficient and represent first-line treatments for the control of this zoonosis. Unfortunately, their implementation in areas or countries where toxocariasis epidemiology is driven by poverty is quite difficult or unrealistic.
The Immediate Hotspot of Microbial Nitrogen Cycling in an Oil-Seed Rape (Brassica campestris L.) Soil System Is the Bulk Soil Rather Than the Rhizosphere after Biofertilization
Authors: Wu Shanghua , Bohu Tsing , Dong Yuzhu , Wang Shijie , Zhao Shijie , Fan Haonan , Zhuang Xuliang
Published: January 2022

Biofertilizers are substances that promote plant growth through the efficacy of living microorganisms. The functional microbes comprising biofertilizers are effective mediators in plant-soil systems in the regulation of nitrogen cycling, especially in nitrification repression. However, the deterministic or stochastic distribution of the functional hotspot where microbes are active immediately after biofertilization is rarely investigated. Here, pot experiments with oil-seed rape (Brassica campestris L.) were conducted with various chemical and biological fertilizers in order to reveal the distribution of the hotspot after each fertilization. A stimulated dynamic of the nitrogen cycling-related genes in the bulk soil inferred that the bulk soil was likely to be the hotspot where the inoculated bacterial fertilizers dominated the nitrogen cycle. Furthermore, a network analysis showed that bulk soil microbial communities were more cooperative than those in the rhizosphere after biofertilization, suggesting that the microbiome of the bulk soils were more efficient for nutrient cycling. In addition, the relatively abundant ammonia-oxidizing bacteria and archaea present in the networks of bulk soil microbial communities further indicated that the bulk soil was the plausible hotspot after the application of the biofertilizers. Therefore, our research provides a new insight into the explicit practice of plant fertilization and agricultural management, which may improve the implementational efficiency of biofertilization.
Special Issue: The Role of Microorganisms in the Evolution of Animals and Plants
Authors: Rosenberg Eugene , Zilber-Rosenberg Ilana
Published: January 2022

It is now well established that all animals and plants harbor abundant and diverse microorganisms, including bacteria, archaea, viruses, and eukaryotic microorganisms [...]
Assessment of Metallurgical Slags as Solar Heat Absorber Particles
Authors: Alkan Gözde , Mechnich Peter , Lucas Hugo , Knoblauch Nicole , Sommerfeld Marcus , Flucht Ferdinand , Pernpeintner Johannes , Sergeev Dmitry , Müller Michael , Friedrich Bernd
Published: January 2022

The use of ceramic solid particle technology in TES-integrated CSP plants offers a high solar-to-electricity ratio and enhanced storage densities, thanks to their high operational temperatures and wide temperature ranges. Metallurgical slags with composition similar to that of the state-of-art bauxite particles can be used as a sustainable and economical secondary raw material to prepare solid particles. In this study, the as-received state and the high-temperature phase and microstructural changes of two fayalite slags from copper and lead production were elucidated by XRD and SEM/EDS methods in a comparative manner. Solid particles were prepared from slags by the oil dropping method, with subsequent heat treatment. Solar-thermal-application-related functional properties of slag particles, such as heat capacity, absorptance, and thermophysical properties, were evaluated by differential scanning calorimetry (DSC), spectrophotometer, heating microscope, and high-temperature compressive tests, respectively. Owing to the formation of more stable Fe-rich phase components and less amount of glassy phase, copper slag is found to be a more promising secondary resource than lead slag in terms of material and functional properties.
Application of Numerical Simulation Methods in Solving Complex Mining Engineering Problems in Dingxi Mine, China
Authors: Hu Boyi , Zhang Qinli , Li Shuai , Yu Haoxuan , Wang Xinmin , Wang Hao
Published: January 2022

In the mining industry, with numerical simulation analysis of stope roof stability, stope exposed area computation, and pillar buckling collapse simulation, backfill body creep damage mechanism research is becoming the most popular method in the field of backfill mining techniques. In this paper, we first summarized and analyzed the current application status and the existing problems of numerical simulation for solving mining engineering technical problems; then, based on the practical engineering problems of mining phosphate rock resources under high and steep rock slopes (HSRS), we carried out a true-3D numerical simulation study for different underground mining methods, to determine the appropriate mining method. Therefore, this paper, taking Dingxi Mine in China as an example, highlights the advantages of the backfill mining method with a high and steep slope; meanwhile, it also points out how to improve the accuracy of a numerical simulation and make it more consistent with the actual situation of the mining engineering application site. This paper only serves as a guide, in order to start a conversation, and we hope many more experts and scholars will become interested and engaged in this field of research.
A Method to Detect Abnormal Gas Dispersion Conditions in Flotation Machines
Authors: Vinnett Luis , Yianatos Juan , Acuña Claudio , Cornejo Iván
Published: January 2022

This short communication presents a methodology to detect abnormal gas dispersion conditions in flotation machines. These abnormal conditions are characterized by the significant presence of cap-shaped bubbles. The approach considers the use of a bubble size analyzer to measure gas dispersion at industrial scale. The detection of abnormal conditions is critical when estimating bubble size by automated software. Otherwise, the estimates are significantly biased, since irregular bubbles are typically removed from the analysis. From the recorded images and the respective black and white representation, the variability of the shadow percentage caused by the bubbles in the vision field, abruptly increases in the presence of cap-shaped bubbles. Experimental conditions with coefficients of variation lower than 60–70% in the shadow percentage represent spherical and spherical-ellipsoidal regimes. In the former, automated bubble sizing software has proved to be sufficiently effective in obtaining reliable results. In the latter, different segmentation techniques have been proposed to obtain satisfactory results. Abnormal conditions are detected under coefficients of variation greater than 80% in the shadow percentage. The presence of cap-shaped bubbles causes inefficiencies in the collection of hydrophobic minerals in the pulp zone as well as disturbances in the separation stage (froth zone). Therefore, the detection of these irregular bubbles is suitable to provide feedback to flotation processes, allowing gas dispersion to be driven towards normal operating conditions.
Sedimentary Facies Controls for Reservoir Quality Prediction of Lower Shihezi Member-1 of the Hangjinqi Area, Ordos Basin
Authors: Anees Aqsa , Zhang Hucai , Ashraf Umar , Wang Ren , Liu Kai , Abbas Ayesha , Ullah Zaheen , Zhang Xiaonan , Duan Lizeng , Liu Fengwen , Zhang Yang , Tan Shucheng , Shi Wanzhong
Published: January 2022

The tight gas reserves in the Hangjinqi area are estimated at 700 × 109 m3. Since the exploration of the Hangjinqi, numerous wells are already drilled. However, the Hangjinqi remains an exploration area and has yet to become a gas field. Identifying a paleo-depositional framework such as braided channels is beneficial for exploration and production companies. Further, braided channels pose drilling risks and must be properly identified prior to drilling. Henceforth, based on the significance of paleochannels, this study is focused on addressing the depositional framework and sedimentary facies of the first member (P2x1) of the lower Shihezi formation (LSF) for reservoir quality prediction. Geological modeling, seismic attributes, and petrophysical modeling using cores, logs, interval velocities, and 3D seismic data are employed. Geological modeling is conducted through structural maps, thickness map, and sand-ratio map, which show that the northeastern region is uplifted compared to northwestern and southern regions. The sand-ratio map showed that sand is accumulated in most of the regions within member-1. Interval velocities are incorporated to calibrate the acoustic impedance differences of mudstone and sandstone lithologies, suggesting that amplitude reflection is reliable and amplitude-dependent seismic attributes can be employed. The Root Mean Square (RMS) attribute confirmed the presence of thick-bedded braided channels. The results of cores and logging also confirmed the presence of braided channels and channel-bars. The test results of wells J34 and J72 shows that the reservoir quality within member-1 of LSF is favorable for gas production within the Hangjinqi area.
Design and Application of a Rock Porosity Measurement Apparatus under High Isostatic Pressure
Authors: Liu Liyu , Li Heping , Zhou Hongbin , Lin Sen , Li Shengbin
Published: January 2022

Rock porosity is a key physical parameter at room temperature and pressure that plays an important role in evaluating reserves of oil and natural gas. Research on rock porosity spans over a hundred years. However, in situ porosity under a high isostatic pressure has not been adequately explored, and the experimental conditions for measuring porosity remain unclear. To investigate the feasibility of porosity measurement under a high isostatic pressure and the optimal choice of experimental conditions for this, we design an experimental apparatus that can achieve isostatic pressure up to 200 MPa to fit the relationship between the void volume of a given sample and the drop in gas pressure in an empty standard chamber. The effect of experimental parameters, such as the initial gas pressure at the inlet, the time needed for the gas to reach equilibrium, and the time needed for vacuuming, on the porosity experiment was examined. A series of porosity experiments under different isostatic pressures of up to 200 MPa were carried out with this apparatus. The results quantitatively verify the degree to which porosity is related to isostatic pressure.
Petrography and Geochemistry of the Intrusive Rocks at the Diorite-Hosted Regnault Au Mineralization
Authors: Mathieu Lucie , MacDonald Francis
Published: January 2022

Archean greenstone belts are renowned for their Au endowment. Gold can be associated with orogenic Au style of mineralization and with diorite-hosted Au-Cu disseminated sulfides interpreted as a porphyry style of mineralization. The Regnault Au project, located in the Frotet–Evans greenstone belt (Superior craton), is a structurally-controlled and diorite-hosted mineralization with an unclear metallogenic model. The aim of this study is to evaluate the fertility of the Regnault granodiorite-diorite-gabbro intrusive. Using whole-rock chemistry and petrological observations, it is concluded that the intrusive suite derives from a differentiated and water-bearing magma extracted from the metasomatized mantle. Amphibole chemistry indicates that the magma was moderately oxidized and that it emplaced at a shallow depth. It is concluded that the Regnault intrusive suite displays several characteristics favorable to the exsolution of magmatic fluids in the upper crust and that the Regnault Au mineralization potentially corresponds to, at least in part, a magmatic-hydrothermal system.
Cu Dynamics in the Rhizosphere of Native Tropical Species: Assessing the Potential for Phytostabilization in Mining-Impacted Soils
Authors: de Oliveira Daniel Pontes , Queiroz Hermano Melo , Perlatti Fabio , Ferreira Amanda Duim , Asensio Verónica , Nóbrega Gabriel Nuto , Otero Xosé Luis , Ferreira Tiago Osório
Published: January 2022

The use of native plants for reforestation and/or remediation in areas contaminated by mining is a technique with low implantation and maintenance costs. The success of this practice depends on the plant species and geochemical processes at the soil–plant interface (e.g., rhizosphere). This study evaluated the potential of spontaneous species for mobilizing and altering mineral and metal dynamics in the rhizosphere of Cu-rich soils resulting from the abandoned Pedra Verde mine in NE Brazil. Rhizosphere and bulk soil samples were collected from five shrubby/arboreal species. The pH, organic matter content, Cu fractionation, mineralogical characterization, and Cu content in the leaves and roots of all studied species were determined. In addition, the bioaccumulation factor (BCF) and translocation factor (TF) were used to evaluate the potential of these species for Cu hyperaccumulation. The Cu concentration in leaf plant tissues varied from 18 to 34 mg kg−1, and all plants presented TF and BCF < 1, indicating that the species were not Cu hyperaccumulators. However, the root exudates induce mineral dissolution, indicating potential Cu accumulation in the roots (from 36 to 249 mg kg−1). Combretum aff. pisoniodes Taub was the species with the greatest potential for decreasing Cu bioavailability and phytostabilization. Our findings indicate the potential of native Brazilian plants for growth in Cu-contaminated soil. These findings may be used for reforestation programs.
Mineralogy of Miocene Petrified Wood from Central Washington State, USA
Authors: Mustoe George E. , Dillhoff Thomas A.
Published: January 2022

Silicified wood occurs abundantly in Middle Miocene flows and sedimentary interbeds of the Columbia River Basalt Group (CRBG) in central Washington State, USA. These fossil localities are well-dated based on radiometric ages determined for the host lava. Paleoenvironments include wood transported by lahars (Ginkgo Petrified Forest State Park), fluvial and palludal environments (Saddle Mountain and Yakima Canyon fossil localities), and standing forests engulfed by advancing lava (Yakima Ridge fossil forest). At all of these localities, the mineralogy of fossil wood is diverse, with silica minerals that include opal-A, opal-CT, chalcedony, and macrocrystalline quartz. Some specimens are composed of only a single form of silica; more commonly, specimens contain multiple phases. Opal-A and Opal-CT often coexist. Some woods are mineralized only with chalcedony; however, chalcedony and macrocrystalline quartz are common as minor constituents in opal wood. In these specimens, crystalline silica filling fractures, rot pockets, and cell lumen may occur. These occurrences are evidence that silicification occurred as a sequential process, where changes in the geochemical environment or anatomical structures affected the precipitation of silica. Fossilization typically began with precipitation of amorphous silica within cell walls, leaving cell lumen and conductive vessels open. Diagenetic transformation of opal-A to opal-CT in fossil wood has long been a widely accepted hypothesis; however, in opaline CRBG specimens, the two silica polymorphs usually appear to have formed independently, e.g., woods in which cell walls are mineralized with opal-A but in which lumen contain opal-CT. Similarly, opal-CT has been inferred to sometimes transform to chalcedony; however, in CRBG, these mixed assemblages commonly resulted from multiple mineralization episodes.