bluuebunny/crossref_metadata_2025 huggingface dataset:
Minke whale hunt and animal welfare
Authors: Knudsen SK , Øen EO , Walløe L
Published: January 2023

Over the last twenty-five years, Norway has given priority to research to improve hunting methods for Minke whales. This has resulted in an increase in the instantaneous death rate (IDR) from about 17% in the early 1980s to about 80% today, and, thus, in a considerable decrease in the time to death (TTD) (Øen 2003). TTD data have been collected by official veterinary inspectors on board Norwegian whaling vessels and reported annually to the International Whaling Commission (IWC) since 1983. Estimates of TTD have been based, predominantly, on the ‘criteria of death in whales’ that were established at an IWC workshop in 1980 (IWC 1980). These criteria in principle require complete immobility, ie a whale can only be pronounced dead if it has stopped moving, the jaw and flippers have slackened, and it is sinking passively. However, as early as 1995 the IWC agreed (IWC 1995) that the criteria were incomplete and sometimes misleading, as they did not allow for reflex movements and seizures that occur in the unconscious state after an animal is stunned. This phenomenon is well-known from the slaughter of domestic animals (for review, see eg Knudsen 2005). The IWC identified a need for a more reliable method of determining the time of onset of permanent insensibility in whales (IWC 1995) and in particular asked for research investigating in detail the trauma, and its consequences, caused by hunting methods.
Physiological and behavioural responses of broilers to controlled atmosphere stunning: implications for welfare
Authors: McKeegan DEF , McIntyre JA , Demmers TGM , Lowe JC , Wathes CM , van den Broek PLC , Coenen AML , Gentle MJ
Published: January 2023

Controlled atmosphere (gas) stunning (CAS) has the potential to improve the welfare of poultry at slaughter but there is a lack of consensus about which gas mixtures are most humane. The aim of this study was to evaluate the welfare consequences of different gas stunning approaches. Individual broilers were exposed to gas mixtures capable of inducing unconsciousness and euthanasia while their behavioural, cardiac, respiratory and neurophysiological responses were measured simultaneously. The approaches investigated included anoxia (N2or Ar with < 2% residual O2), hypercapnic anoxia (30% CO2in Ar, 40% CO2in N2) and a biphasic method (40% CO2, 30% O2, 30% N2for 60 s followed by 80% CO2in air). Evaluation of the welfare implications of each approach centred on the likelihood of them inducing negative states or experiences during the conscious phase. Hypercapnic mixtures were associated with strong respiratory responses, while anoxic mixtures induced vigorous wing flapping. Electroencephalogram analysis using the correlation dimension (a non-linear measure of complexity) suggested that anoxic wing flapping occurred during periods in which a form of consciousness could not be excluded. Hypercapnic hyperoxygenation (biphasic approach) exacerbated respiratory responses but eliminated the possibility of vigorous behavioural responses occurring during a conscious phase. The relative importance of respiratory discomfort versus the potential to induce significant distress due to convulsive wing flapping and associated trauma is a matter for debate. We argue that respiratory discomfort is unpleasant but may be preferable to the risk of vigorous wing flapping and associated injury while conscious in poultry during CAS.
FAWC Report on Stockmanship and Farm Animal Welfare
Authors: Wickens S
Published: January 2023

“Stockmanship is the single most important influence on the welfare of farm animals”, thus begins this report from the UK's Farm Animal Welfare Council into the role and scope of stockmanship, as it pertains to ensuring high standards of animal welfare, and the provision of effective education and training for livestock farmers and stockmen within the UK.
Survey of slaughter plants from an animal welfare standpoint in Eastern Turkey
Authors: Sabuncuoglu N , Coban O , Bayraktar M , Elmali M , Ekici K , Ceylan ZG
Published: January 2023

Turkey, a candidate for EU membership, has a large livestock sector in the east of the country with considerable numbers of slaughter animals. This survey examined welfare practices in fifteen slaughter plants located in the region surrounding five cities (Bingol, Erzurum, Elazig, Kars and Van) in eastern Turkey. Evaluation criteria were developed on the basis of minimum requirements of EU animal welfare legislation for slaughter animals (Anonymous 1979). The plants, which were designed and used for cattle and sheep slaughter, were visited and slaughter procedures observed in April 2006. The criteria and results are presented in Table 1.
Juvenile farmed mink (Mustela vison) with additional access to swimming water play more frequently than animals housed with a cylinder and platform, but without swimming water
Authors: Vinke CM , van Leeuwen J , Spruijt BM
Published: January 2023

The opportunity to perform play behaviour may be an important ontogenic activity that stimulates behavioural variability and may enhance an individual's coping capacity later in life. Play behaviour in juveniles may be enhanced by the presence of cage enrichments relevant to the animal's motivations and natural behavioural repertoire. The present study aimed to investigate play behaviour in juvenile farmed mink reared and housed with the cage enrichments standard for the Dutch housing system (ie a cylinder and platform) and in an experimental group of animals with the same standard enrichments but with additional access to swimming water. Juvenile mink with access to swimming water played significantly more in the main cage than mink reared and housed with the cylinder and platform but without swimming water. The results suggest that swimming water presents the animals with biologically relevant stimuli that directly or indirectly influence the development of play behaviour. Specific implications for the animals' long-term welfare are discussed. Future studies should elucidate the effects of juvenile play on the occurrence of abnormal behavioural patterns in adulthood more precisely and more thoroughly.
Modifying the behaviour of singly caged baboons: evaluating the effectiveness of four enrichment techniques
Authors: Bourgeois SR , Brent L
Published: January 2023

Techniques to reduce or prevent behavioural disturbances in singly caged primates vary in form and effectiveness, with some behaviours being exceptionally resistant to treatment. Seven singly caged adolescent male olive hybrid baboons (Papio hamadryas anubis) were selected for behavioural intervention because of their severe abnormal behaviour. A continuous, all-occurrence sampling method yielded mean durations of abnormal and normal behaviour throughout the 10-week study. Analysis of baseline behaviour verified substantial durations of abnormal behaviour (mean = 9.80 min per 30-min observation period). We tested the effectiveness of four enrichment techniques: positive reinforcement training (PRT), food enrichment, non-food enrichment, and social enrichment (pair/trio housing). Each of the four enrichment conditions was implemented for a two-week period, with 10 30-min observations conducted per subject. We used repeated-measures analysis of variance to examine differences in behaviour durations between baseline and each enrichment condition. The social enrichment condition resulted in the most positive behavioural changes, including increased social behaviour and near elimination of abnormal behaviours (mean = 0.69 min per 30-min observation). Significant reductions in total abnormal behaviour levels were also found for other types of enrichment, but only social enrichment and PRT were effective in reducing whole-body stereotypies. Cage-directed and self-directed behaviours significantly decreased, whereas activity levels significantly increased during all enrichment conditions. The results of this project indicate that animate enrichment (human or conspecific stimulation), as opposed to inanimate enrichment, provides optimal means of behaviour modification for singly caged baboons. These findings have substantial implications for the welfare of captive primates in promoting successful therapeutic approaches for the behavioural management of laboratory primate species and for allocating limited enrichment resources.
The welfare impact of gavaging laboratory rats
Authors: Bonnichsen M , Dragsted N , Hansen AK
Published: January 2023

Gavaging (oral dosing) has previously been shown to have only a short-term effect on behavioural parameters in the laboratory rat. The aim of this study was to determine if the gavaging of laboratory rats influenced their heart rate, blood pressure and body temperature, and if so, whether the duration of this impact correlated with the volume gavaged. The three stress parameters were measured using telemetric transponders placed in the abdomen of eight female Sprague-Dawley (Mol:SPRD) rats. Using a Latin Square cross-over design, the rats were gavaged with three different doses of barium sulphate (4, 10 and 40 ml kg–1); in addition, there was a control of no dose, only insertion of the tube. The heart rate, blood pressure and body temperature of the rats were monitored continuously for 4 h after dosing and again for 1 h, 24 h after dosing. The gavaging of laboratory rats was shown to induce an acute reaction: after 30 min, blood pressure and heart rate were significantly higher than before gavaging, and body temperature was significantly higher 60 min after gavaging — indicators of stress levels comparable to those of other basic experimental procedures. A significant correlation between heart rate and dosage was observed until 10 min after gavaging. This indicates that the dosage gavaged is of only minor importance in causing stress, and only important for the most acute reaction. However, because of the resistance and discomfort observed when administering a 40 ml kg–1dose, this dose should be administered only with caution.
Housing environment alters delayed-type hypersensitivity and corticosterone concentrations of individually housed male C57BL/6 mice
Authors: Neigh GN , Bowers SL , Korman B , Nelson RJ
Published: January 2023

Housing conditions can alter both the physiology and behaviour of laboratory animals. Forced-air-ventilated micro-isolation systems increase the efficient use of space, decrease the incidence of disease among laboratory rodents, and provide better working conditions for animal care staff; however, such systems can increase breeding variability and mortality. We examined the possibility that stressors associated with automated housing conditions evoke subtle changes among immune, endocrine, and behavioural parameters in mice housed in a static versus a forced-air-ventilated micro-isolation system. In addition, we assessed the effects of housing in the forced-air-ventilated micro-isolation system both with and without the use of an automatic watering system. Housing in the forced-air-ventilated micro-isolation system, using the automatic watering system, suppressed delayed-type hypersensitivity (DTH) responses, a measure of cell mediated immune function, compared with the responses of mice housed in static cages. Hypothalamic–pituitary–adrenal axis function was also altered by housing in the forced-air-ventilated micro-isolation system with the use of the automatic watering system, such that mice in this housing system had lower resting corticosterone concentrations and increased reactivity to restraint. Despite these changes in corticosterone, housing condition did not alter activity level or exploratory, anxiety-like, or depressive-like behaviours. These results suggest that investigators should carefully consider housing conditions in studies of immune and endocrine function.
Effective searching of the scientific literature for alternatives: search grids for appropriate databases
Authors: Hart LA , Wood MW , Weng H-Y
Published: January 2023

Researchers searching for alternatives to painful procedures that involve animals may find that the dispersed relevant literature and the array of databases make the search challenging and even onerous. This paper addresses a significant gap that exists for researchers, in identifying appropriate databases to use when searching for specific types of information on alternatives. To facilitate the efficient and effective searching by users, and to ensure compliance with new requirements and improved science, we initiate an evolving guide comprising search grids of database resources organised by animal models and topics (http://www.vetmed.ucdavis.edu/Animal_Alternatives/databaseapproach.html). The search grids present organised lists of specific databases and other resources for each animal model and topic, with live links. The search grids also indicate resources that are freely available worldwide, and those that are proprietary and available only to subscribers. The search grids are divided into two categories: ‘animal models’ and ‘topics’. The category ‘animal models’ comprises: animal model selections; mice; rodents — rats/guinea pigs/hamsters; rabbits; dogs, cats; farm animals, sheep, swine; non-human primates; fish, frogs, aquatic; and exotics. The category ‘topics’ comprises: husbandry; behaviour; euthanasia; toxicity; monoclonal antibodies; teaching; endpoints; disease models; analgesia/anaesthesia; emerging technologies; strategies for specific intervention procedures; and standard operating procedures (for example, drawing blood, behavioural training, transportation, handling, restraint and identification). Users are provided with a selected list of linked resources relevant to their particular search. Starting with an appropriate database that covers the type of information that is being sought is the first step in conducting an effective search that can yield useful information to enhance animal welfare.
The application of Russell and Burch's Three Rs in commercial livestock experimentation
Authors: Colditz IG
Published: January 2023

The inclusion of Russell and Burch's Three Rs (replacement, reduction and refinement) in guidelines, codes of practice and law reflects their current position as the guiding principles of ethical assessment of research involving animals. This article explores some activities within the contemporary livestock industry that constitute the experimental use of animals on a local and global scale. The elucidation of correlated responses during trait selection in genetic improvement programs provides one example of experiments occurring within the commercial livestock industry. This experimentation is largely conducted without scrutiny of its conformity to the Three Rs. Experimentation to improve the management of the livestock industry is consistent with the principle of refinement, and experimentation to increase productivity per unit of livestock is consistent with the principle of reduction; however, experimentation to increase total livestock production conflicts with the principle of replacement. Some approaches regarding the appraisal of the ethics of research involving animals, which could avoid arbitrary boundaries associated with the location or purpose of experimentation, are considered together with the relationship between experimentation and other anthropogenic impacts on animals.
Motivation for group housing in gestating sows
Authors: Kirkden RD , Pajor A
Published: January 2023

It has been argued that the welfare of gestating sows is higher in groups than singly in stalls, in part because group housing offers them more space and social contact. This study set out to ascertain how important access to a group pen was to dominant sows housed in stalls, using a measure of motivation. Subjects were trained to perform a panel-pressing task, then housed in a stall and permitted each day to work for a day's access to a fully slatted group pen containing two familiar, subordinate sows at a stocking density of 2.7m2per pig. Social ranking was determined by observations at mixing and from feed competition tests. The fixed-ratio schedule was increased daily and the highest schedule reached (the reservation price) was used as a measure of motivational strength. To interpret this measure, it was compared with the highest schedule that subjects reached when working for access to the last 1/16thof their estimatedad libitumdaily food intake after having consumed the first 15/16thsfree. Sixteen subjects were tested, eight working for access to the group pen first and eight for access to the food first. Seven subjects yielded useable data: four reached a higher schedule working for food and three reached a higher schedule working for the group pen. Overall, subjects attached no more importance to a day's access to the group pen than to the last 1/16thof their estimatedad libitumfood intake. It is likely that the subjects were close to satiation when working for food because consumption frequently fell substantially short of the ‘ad libitum‘ allowance. These results suggest that dominant, stall-housed sows are only weakly motivated to gain access to a fully slatted group pen, although motivation might be higher when deprived of access to the group pen for longer than one day, if tested at a different time of day or if the quality of the group space was improved; these three possibilities still need to be tested.
Can non-invasive glucocorticoid measures be used as reliable indicators of stress in animals?
Authors: Lane J
Published: January 2023

Refinement techniques are being increasingly employed in all fields of animal research to try to ensure that the highest standards of welfare are upheld. This review concerns one of the main emerging techniques for the assessment of welfare itself, namely the non-invasive measurement of glucocorticoids (GCs) as indicators of stress. The paper is divided into three sections. The first discusses the relationship between GCs and stress. The second section considers whether factors other than stress are linked to rises in GCs, eg exercise, oestrus cycle and diet. The final part examines the reliability of the non-invasive techniques that measure GCs from samples of saliva and faeces. Although it is important to take into account some caveats associated with the methodologies employed, it is concluded, nevertheless, that these techniques can give accurate and reliable information regarding the welfare status of an individual or group of animals without the procedures themselves causing any kind of distress to the subjects.
Song development in birds: the role of early experience and its potential effect on rehabilitation success
Authors: Spencer KA , Harris S , Baker PJ , Cuthill IC
Published: January 2023

Environmental conditions during the early life stages of birds can have significant effects on the quality of sexual signals in adulthood, especially song, and these ultimately have consequences for breeding success and fitness. This has wide-ranging implications for the rehabilitation protocols undertaken in wildlife hospitals which aim to return captive-reared animals to their natural habitat. Here we review the current literature on bird song development and learning in order to determine the potential impact that the rearing of juvenile songbirds in captivity can have on rehabilitation success. We quantify the effects of reduced learning on song structure and relate this to the possible effects on an individual's ability to defend a territory or attract a mate. We show the importance of providing a conspecific auditory model for birds to learn from in the early stages post-fledging, either via live- or tape-tutoring and provide suggestions for tutoring regimes. We also highlight the historical focus on learning in a few model species that has left an information gap in our knowledge for most species reared at wildlife hospitals.
Validation of broiler chicken gait scoring training in Thailand, Brazil and New Zealand
Authors: Butterworth A , Knowles TG , Whittington P , Matthews L , Rogers A , Bagshaw CS
Published: January 2023

Broiler chickens are the most numerous terrestrial farmed animals on earth. These birds can suffer a range of pathologies which result in lameness, and one technique for assessing the impact of lameness on the individual bird, and on the flock has been gait scoring. This paper describes a study where the results of the same training programme for validated gait scoring are compared for groups of assessors in Thailand, Brazil and New Zealand. A uniform course of training carried out in the classroom, through video assessment and through on-farm training was carried out in the countries named; a total of 95 persons being trained overall. A measure of agreement with the reference scale for the assessors' numerical responses was carried out using a Cohen's Kappa test, and the results indicated significant differences in the ‘origin position’ ie the view taken by the assessors of the impact of lameness on the bird before training had taken place, but a high degree of consistency in the response to training. The study demonstrated that gait scoring can be learned by assessors from different geographical and social backgrounds, and that the ‘rate of learning’ – the improvement in performance in relation to a standard, was similar between the countries studied. The ‘origin’ and ‘final’ position of the assessors pre- and post-training, from the different countries in relation to the reference standard is discussed.
Testing different methods to evaluate pig welfare on farm
Authors: Courboulay V , Foubert C
Published: January 2023

Feasibility and validity of protocols used to assess welfare were evaluated in two fattening rooms in twenty farms, chosen according to the group size, pen shape, floor and space per animal. The two rooms contained animals at the beginning or at the end of the fattening period, respectively. Behaviour was recorded either through direct observations of the pens (3 times per minute, at 5 min intervals), 15 minutes after entering the room (B1) or after the removal of an object given at the end of the visit (B3). The third protocol consisted of a 2 minute observation inside the pen (B2). To evaluate the human-animal interaction, the time to adapt to the presence of the operator in the corridor (HA1) was measured, as well as the reaction of the group when the operator entered the pen (HA2) or walked slowly through the pen (HA3), the time taken by the first five pigs to approach the operator (HA4) and the reaction of the animals when the operator tried to catch an ear (HA5). Lesion scoring was carried out inside the pen on a sample of 60 individual fatteners or at pen level on most of the pigs from the corridor. The occurrence of the main active behaviours is assessed similarly over time by B1and B3. Behavioural observations are then possible in an on-farm welfare assessment. Lesion scoring from the corridor tends to under estimate the number of lesions (scratches, tail wounds). The HA3test appears to be the best one to evaluate the relationship towards humans but has to be validated.
Results of the Animal Needs Index (ANI-35L) compared to animal-based parameters in free-range and organic laying hen flocks in Austria
Authors: Zaludik K , Lugmair A , Baumung R , Troxler J , Niebuhr K
Published: January 2023

The aim of this study was a comparison of Animal Needs Index (ANI) data, derived from annual inspections by a control agency, with data collected from 164 selected on-farm flocks concerning feather damage, injuries, egg production, mortality, bodyweight, foot pad dermatitis, keel bone deviations and reactions towards humans (eg flock showed marked avoidance when the observer walked through the hen-house [yes/no]).

Analysis of data showed a low number of significant correlations with total ANI scores and category scores: 1) Locomotion, 2) Social interaction, 3) Flooring, 4) Light, air and noise and 5) Stockmanship. Correlations found were low and total ANI score showed only a positive correlation with egg production at week 52. Category 3 scores correlated positively with egg production at week 70 and negatively with the percentage of hens with featherless areas and total pecking injuries. Category 5 scores showed positive correlations with egg production at week 52 as well as week 70 and negative correlations with mortality at week 52, the percentage of hens with featherless areas, pecking injuries < 0.5 cm and total pecking injuries.

Flocks showing marked avoidance had a lower total ANI score and lower category 3 and category 5 scores.

In conclusion, welfare-related animal-based parameters are poorly reflected by the ANI-35-L/2001. To assess animal welfare more adequately, animal-based parameters have to be considered additionally in a welfare assessment scheme.
Formal animal-based welfare assessment in UK certification schemes
Authors: Main DCJ , Whay HR , Leeb C , Webster AJF
Published: January 2023

Providing assurance of improvement in welfare outcomes is the desire of some UK certification schemes. However, this is only likely to be achieved if such schemes monitor welfare outcomes rather than rely on defining welfare resources that should be provided. The University of Bristol has developed protocols for the on-farm evaluation of animal-based welfare parameters for incorporation into certification schemes. The assessment system is designed to provide credible (repeatable, valid and feasible) evidence for assessment of compliance with welfare standards in organic and conventional farming systems. For issues identified as causing potential concern the assessor is encouraged to conduct further investigations of the relevant resource standards and, where appropriate, management requirements. Furthermore the assessment tool enables certification bodies and relevant third parties to monitor the ability of schemes to deliver good welfare outcomes. Finally, it provides a mechanism for farmers to review their own health and welfare performance. The practical application of these systems is now being pursued by some UK certification bodies.
Self-evaluation of animal welfare by the farmer: a report of application on Austrian cattle farms
Authors: Ofner E , Schmid E , Schröck E , Troxler J , Hausleitner A
Published: January 2023

It is not sufficient to merely publish regulations and strictly control them to improve the living conditions of farm animals. The farmer must receive precise information, understand the individual measures and be able to act with personal responsibility. A new animal welfare law has been in force in Austria since the 1st of January 2005. A system of self-evaluation is being developed for putting these new legal requirements into practice. The aim of this self-evaluation system is to enable every farmer to personally check his animal housing system against the given standards. This independent work motivates the farmer and makes him aware of animal welfare issues. This new strategy for the implementation of animal welfare law was originally developed, tested and found to be successful by the Federal Administration of the Austrian Province of Vorarlberg. Self-evaluation is to be carried out by means of specific checklists and manuals. Checklists and manuals are currently being drawn up for cattle, pigs, poultry, sheep and goats. The checklists and manuals will include all of the requirements of Austrian animal welfare legislation and will be formulated in easily understandable questions with ‘yes’ or ‘no’ answers. The manuals will describe the methods for assessing legal requirements on the farm as well as the conditions for compliance with legislation, and give advice on how to improve the state of animal welfare on the farm. This paper gives an example of the self-evaluation system for cattle farming.
Bridging consumer perception and on-farm assessment of animal welfare
Authors: Napolitano F , De Rosa G , Caporale G , Carlucci A , Grasso F , Monteleone E
Published: January 2023

The present study aims to assess the relationship between consumer perception and on-farm assessment of animal welfare performed using the Animal Needs Index 35L (ANI). Two tie-stall, 2 straw yard and 2 cubicle farms were scored by trained assessors using the ANI and filmed to produce six 3.5 min videos. Each of them contained 4 clips: barn overall view, feeding, milking and individual animals. Ten untrained observers were asked to elicit terms describing how they perceived the observed farming systems to affect animal welfare using Free Choice Profiling (FCP). Data from FCP were subjected to Generalised Procrustes Analysis (GPA). GPA showed a significant consensus among observers. Observers characterised the first dimension with terms ranging from constrictive to comfortable conditions and the second one in terms of cleanliness/dirtiness. Principal Component Analysis (PCA) was conducted using the scores of the farms on the first two dimensions of GPA and the data gathered through the ANI. The first dimension of GPA (Comfortable), ANI's sheet 1 (Locomotion), sheet 2 (Social interaction) and sheet 6 (Total score) showed high loadings on the first component of PCA, whereas the second dimension of GPA (Clean), sheet 3 (Flooring) and sheet 4 (Stockmanship) were correlated with the second component of PCA. We concluded that FCP may be used to elicit lay person perception of welfare-related characteristics of dairy cattle farming systems, thus providing a tool to study the relationships between consumer perception and on-farm assessment of animal welfare.
Using the Piglet Scream Test to enhance piglet survival on farms: data from outdoor sows
Authors: Held S , Mason G , Mendl M
Published: January 2023

The point of the Piglet Scream Test is to identify those sows that increase the survival of their piglets through good maternal behaviour. For this to work, sows must be shown to differ in their test responsiveness, for differences to be consistent across parities and for responsiveness to be associated with piglet survival. Our data provide some first evidence that outdoor sows, kept under commercial production conditions, differ consistently from each other in their test responsiveness across parities. However, there was no evidence that this was associated with the survival of their litters. This, together with the lack of consistent evidence from other studies for a relationship between Piglet Scream Test responsiveness and piglet survival and concerns about its possible causes, cautions against the use of the test to enhance piglet survival on farms without further study. The paper highlights lack of standardisation of the test and pseudoreplication as concerns.
Anthropomorphism and anthropocentrism as influences in the quality of life of companion animals
Authors: Bradshaw JWS , Casey RA
Published: January 2023

Since animal minds are private, so their perception of their own quality of life (QoL) must be also. Anthropocentrism, the interpretation of reality exclusively in terms of human values and experience, has to be guarded against in any assessment of animal welfare; for domestic pets, misapprehensions about their olfactory and cognitive abilities appear to present the greatest challenge to their welfare. Anthropomorphism, the attribution of human qualities to animals, presents a particular problem when considering companion animals, since most bonds between owners and their pets appear to be based upon a perception of the pet as almost human. Many owners report that their dogs, cats and horses are capable of feeling complex emotions, such as pride and guilt, that require a level of self-awareness that has been difficult to demonstrate even in chimpanzees. Such beliefs appear to contribute to the development of behavioural disorders in pets; for example, clinical experience suggests that the application of punishment by owners who attribute ‘guilt’ to their animals may unwittingly lead to compromised welfare. Anthropomorphic owners are also likely to be poor proxies for reporting their pets' QoL.
Attitudes of Turkish veterinarians towards animal welfare
Authors: Sabuncuoglu N , Coban O
Published: January 2023

A survey was carried out to examine the attitudes of Turkish veterinarians towards animal welfare issues. The email questionnaire consisted of three sections of statements with a five-point Likert scale for choices of answer. The first section included statements examining the attitudes of Turkish veterinarians to animal welfare issues relating to European Union (EU) Legislation (93/119/EC, 95/29/EC, 2002/4EC and Council Regulation 1/2005). In the second, statements were designed to ascertain veterinarian attitudes towards the recently-passed Animal Protection Law, TR-5199. The statements in the last section were designed to assess the respondents' personal beliefs on a variety of welfare topics. The survey was sent to 615 veterinarians and the response rate was 40.2%. Turkish veterinarians expressed considerable support for the implications of animal welfare with the exception of statements regarding ‘stunning of ruminants pre-slaughter’, ‘phasing out of battery cages for poultry’ and ‘not operating on animals for aesthetic purposes’. In addition, they did not agree with the statements related to ‘ethological needs of farm animals’ and ‘effectiveness of EU laws and legislation in Turkey’. Females had higher mean values than males. The results of the survey indicated that significant concern for animal welfare issues is seen in the Turkish veterinarian population. Although the process of becoming a fully-integrated member of the EU will not occur rapidly, the influence of veterinarians could potentially enhance animal welfare in Turkey.
A pilot study to assess whether high expansion CO2-enriched foam is acceptable for on-farm emergency killing of poultry
Authors: Gerritzen MA , Sparrey J
Published: January 2023

This pilot experiment was conducted to ascertain whether CO2-enriched high expansion foam could be an acceptable and efficient alternative in emergency killing of poultry. This method could have wide-ranging applications but with particular emphasis on small (backyard) flocks, free-range sheds or open (naturally-ventilated) housings. The objectives of the study were as follows: 1) to determine whether the injection of foam and being covered with foam leads to fear or panic reactions in birds; 2) to determine the time taken to render birds unconscious and dead and 3) to determine whether any pathological abnormalities are observed post mortem. Six laying hens were individually exposed to increasing levels of CO2 foam with an expansion rate of 300:1. The test box containing individual birds filled with foam within 30 s. During foaming, two out of six birds tried to escape from the test box (1–2 attempts per bird). Apart from displaying greater alertness, birds showed no aversive reactions to the CO2 foam. Twenty-to-thirty seconds after being covered with foam, five of the six birds demonstrated one or two forcable or convulsive movements. Movement patterns and muscle jerks immediately following this convulsive movement led us to believe that birds lost consciousness at this moment and, within approximately three minutes, all birds had ceased to have a heartbeat. Macroscopic post mortem examination of the birds revealed no abnormalities and microscopic examination showed moderate bronchiolar bleeding and a small amount of alveolar bleeding. After assessing behavioural parameters, measurements of heart rate and pathological data, it is our conclusion that CO2 foam has the potential to be an acceptable method of killing poultry. It is advisable for this method to be examined on a larger scale in order to assess the implications of physiological (EEG and ECG) measurements on welfare.
The Effects of Intrusion on the Behaviour of Caged Laboratory Rats (Rattus Norvegicus): Consequences for Welfare
Authors: Barclay R
Published: January 2023

This experiment examines the consequences and welfare implications of the introduction of new rats to established (resident) caged laboratory rat groups. The effects on investigatory and aggressive behaviours of the established and the newly introduced rats were measured. The aggressive status within each established resident group was known prior to the introduction; the status of each intruder rat was also known. Single-sex groups of both male and female residents and intruders were studied. Two important conclusions arise from the results of the experiment. First, contrary to reports in a number of publications, there were no effects of male resident social status on their investigatory or aggressive behaviour towards male intruder rats. There was, however, a significant effect of social status among female residents on their investigatory behaviour towards intruders; unexpectedly, it was the subordinates followed by the dominant animals that showed the highest investigatory response. Second, aggression by intruders of both sexes depended upon their social status in their resident group. In the case of male intruders, aggressive behaviour towards residents was a reflection of their previous social status irrespective of how much aggression they received from residents. Among females, a similar pattern emerged, with the dominant females initiating the most aggression; however, this behaviour was dependent upon how much aggression was received from residents and on the resident’s social status, with resident subordinates and dominants responding with the most aggressive behaviour. The results are interpreted in terms of putative competitive strategies adopted by rats in a confined (cage) environment, and their welfare implications are discussed.
Immodest flirt or competent governor: translating gender in colonial and post-colonial South Asian historiography
Authors: Khoja Neelam
Published: January 2023

This article examines how an eighteenth-century woman, Mughlani Begum, is depicted in the most informative contemporary Persian auto/biography and how the descriptions, anecdotes, and analysis of her life contained therein, including the brief period she was Punjab's governor, changed as the primary source was translated into or summarised in English. The original Persian and colonial English translations and histories are read alongside an Urdu history of the Punjab, which begs the question: why was the life of a female governor reduced to that of an ‘immodest flirt’ in English sources, while her identity is incredibly nuanced in Persian and Urdu sources? Indeed, post-colonial historians writing in English rarely reference Persian originals: hence, they reproduce what colonial-period English writers before them said, and they completely ignore Urdu histories. While it is nearly impossible to understand the reasons why historians writing in English choose to depict Mughlani Begum in such a flattened way, we can be more critical of our readings of histories written in English, especially when original accounts are available. This article argues for consideration of how transmission of knowledge, language politics, and gender biases inflect historiography and misrepresent historical events and people of the Punjab—especially women.
Observations of Gravity Wave Refraction and Its Causes and Consequences
Authors: Geldenhuys M. , Kaifler B. , Preusse P. , Ungermann J. , Alexander P. , Krasauskas L. , Rhode S. , Woiwode W. , Ern M. , Rapp M. , Riese M.
Published: January 2023

Horizontal gravity wave (GW) refraction was observed around the Andes and Drake Passage during the SouthTRAC campaign. GWs interact with the background wind through refraction and dissipation. This interaction helps to drive midatmospheric circulations and slows down the polar vortex by taking GW momentum flux (GWMF) from one location to another. The SouthTRAC campaign was composed to gain improved understanding of the propagation and dissipation of GWs. This study uses observational data from this campaign collected by the German High Altitude Long Range research aircraft on 12 September 2019. During the campaign a minor sudden stratospheric warming in the southern hemisphere occurred, which heavily influenced GW propagation and refraction and thus also the location and amount of GWMF deposition. Observations include measurements from below the aircraft by Gimballed Limb Observer for Radiance Imaging of the Atmosphere and above the aircraft by Airborne Lidar for the Middle Atmosphere. Refraction is identified in two different GW packets as low as ≈4 km and as high as 58 km. One GW packet of orographic origin and one of nonorographic origin is used to investigate refraction. Observations are supplemented by the Gravity‐wave Regional Or Global Ray Tracer, a simplified mountain wave model, ERA5 data and high‐resolution (3 km) WRF data. Contrary to some previous studies we find that refraction makes a noteworthy contribution in the amount and the location of GWMF deposition. This case study highlights the importance of refraction and provides compelling arguments that models should account for this.
Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation
Authors: Fang Zhaoyuan , Han Xiangkun , Chen Yueqing , Tong Xinyuan , Xue Yun , Yao Shun , Tang Shijie , Pan Yunjian , Sun Yihua , Wang Xue , Jin Yujuan , Chen Haiquan , Hu Liang , Hui Lijian , Li Lin , Chen Luonan , Ji Hongbin
Published: January 2023

Lkb1 deficiency confers the Kras-mutant lung cancer with strong plasticity and the potential for adeno-to-squamous transdifferentiation (AST). However, it remains largely unknown how Lkb1 deficiency dynamically regulates AST. Using the classical AST mouse model (KrasLSL-G12D/+;Lkb1flox/flox, KL), we here comprehensively analyze the temporal transcriptomic dynamics of lung tumors at different stages by dynamic network biomarker (DNB) and identify the tipping point at which the Wnt signaling is abruptly suppressed by the excessive accumulation of reactive oxygen species (ROS) through its downstream effector FOXO3A. Bidirectional genetic perturbation of the Wnt pathway using two different Ctnnb1 conditional knockout mouse strains confirms its essential role in the negative regulation of AST. Importantly, pharmacological activation of the Wnt pathway before but not after the tipping point inhibits squamous transdifferentiation, highlighting the irreversibility of AST after crossing the tipping point. Through comparative transcriptomic analyses of mouse and human tumors, we find that the lineage-specific transcription factors (TFs) of adenocarcinoma and squamous cell carcinoma form a “Yin-Yang” counteracting network. Interestingly, inactivation of the Wnt pathway preferentially suppresses the adenomatous lineage TF network and thus disrupts the “Yin-Yang” homeostasis to lean towards the squamous lineage, whereas ectopic expression of NKX2-1, an adenomatous lineage TF, significantly dampens such phenotypic transition accelerated by the Wnt pathway inactivation. The negative correlation between the Wnt pathway and AST is further observed in a large cohort of human lung adenosquamous carcinoma. Collectively, our study identifies the tipping point of AST and highlights an essential role of the ROS-Wnt axis in dynamically orchestrating the homeostasis between adeno- and squamous-specific TF networks at the AST tipping point.
The HAPSTR2 retrogene buffers stress signaling and resilience in mammals
Authors: Amici David R. , Cingoz Harun , Alasady Milad J. , Alhayek Sammy , Phoumyvong Claire M. , Sahni Nidhi , Yi S. Stephen , Mendillo Marc L.
Published: January 2023

We recently identified HAPSTR1 (C16orf72) as a key component in a novel pathway which regulates the cellular response to molecular stressors, such as DNA damage, nutrient scarcity, and protein misfolding. Here, we identify a functional paralog to HAPSTR1: HAPSTR2. HAPSTR2 formed early in mammalian evolution, via genomic integration of a reverse transcribed HAPSTR1 transcript, and has since been preserved under purifying selection. HAPSTR2, expressed primarily in neural and germline tissues and a subset of cancers, retains established biochemical features of HAPSTR1 to achieve two functions. In normal physiology, HAPSTR2 directly interacts with HAPSTR1, markedly augmenting HAPSTR1 protein stability in a manner independent from HAPSTR1’s canonical E3 ligase, HUWE1. Alternatively, in the context of HAPSTR1 loss, HAPSTR2 expression is sufficient to buffer stress signaling and resilience. Thus, we discover a mammalian retrogene which safeguards fitness.
Estimating conformational landscapes from Cryo-EM particles by 3D Zernike polynomials
Authors: Herreros D. , Lederman R. R. , Krieger J. M. , Jiménez-Moreno A. , Martínez M. , Myška D. , Strelak D. , Filipovic J. , Sorzano C. O. S. , Carazo J. M.
Published: January 2023

The new developments in Cryo-EM Single Particle Analysis are helping us to understand how the macromolecular structure and function meet to drive biological processes. By capturing many states at the particle level, it is possible to address how macromolecules explore different conformations, information that is classically extracted through 3D classification. However, the limitations of classical approaches prevent us from fully understanding the complete conformational landscape due to the reduced number of discrete states accurately reconstructed. To characterize the whole structural spectrum of a macromolecule, we propose an extension of our Zernike3D approach, able to extract per-image continuous flexibility information directly from a particle dataset. Also, our method can be seamlessly applied to images, maps or atomic models, opening integrative possibilities. Furthermore, we introduce the ZART reconstruction algorithm, which considers the Zernike3D deformation fields to revert particle conformational changes during the reconstruction process, thus minimizing the blurring induced by molecular motions.
TLR3 forms a laterally aligned multimeric complex along double-stranded RNA for efficient signal transduction
Authors: Sakaniwa Kentaro , Fujimura Akiko , Shibata Takuma , Shigematsu Hideki , Ekimoto Toru , Yamamoto Masaki , Ikeguchi Mitsunori , Miyake Kensuke , Ohto Umeharu , Shimizu Toshiyuki
Published: January 2023

Toll-like receptor 3 (TLR3) is a member of the TLR family, which plays an important role in the innate immune system and is responsible for recognizing viral double-stranded RNA (dsRNA). Previous biochemical and structural studies have revealed that a minimum length of approximately 40–50 base pairs of dsRNA is necessary for TLR3 binding and dimerization. However, efficient TLR3 activation requires longer dsRNA and the molecular mechanism underlying its dsRNA length-dependent activation remains unknown. Here, we report cryo-electron microscopy analyses of TLR3 complexed with longer dsRNA. TLR3 dimers laterally form a higher multimeric complex along dsRNA, providing the basis for cooperative binding and efficient signal transduction.
The power of microscopic nonclassical states to amplify the precision of macroscopic optical metrology
Authors: Ge Wenchao , Jacobs Kurt , Zubairy M. Suhail
Published: January 2023

It is well-known that the precision of a phase measurement with a Mach-Zehnder interferometer employing strong classic light can be greatly enhanced with the addition of weak nonclassical light. In the context of quantifying nonclassicality, the amount by which a nonclassical state can enhance precision in this way has been termed its ’metrological power’. To-date, the enhancement provided by weak nonclassical states has been calculated only for specific measurement configurations. Here we are able to optimize over all measurement configurations to obtain the maximum enhancement that can be achieved by any single or multi-mode nonclassical state together with strong classical states, for local and distributed quantum metrology employing any linear or nonlinear single-mode unitary transformation. Our analysis reveals that the quantum Fisher information for quadrature-displacement sensing is the sole property that determines the maximum achievable enhancement in all of these different scenarios, providing a unified quantification of the metrological power.