bluuebunny/crossref_metadata_2025 huggingface dataset:
Autoimmune Diseases of Digestive Organs—A Multidisciplinary Challenge: A Focus on Hepatopancreatobiliary Manifestation
Authors: Kunovsky Lumir , Dite Petr , Jabandziev Petr , Kala Zdenek , Vaculova Jitka , Andrasina Tomas , Hrunka Matej , Bojkova Martina , Trna Jan
Published: December 2021

It is well known that some pathological conditions, especially of autoimmune etiology, are associated with the HLA (human leukocyte antigen) phenotype. Among these diseases, we include celiac disease, inflammatory bowel disease, autoimmune enteropathy, autoimmune hepatitis, primary sclerosing cholangitis and primary biliary cholangitis. Immunoglobulin G4-related diseases (IgG4-related diseases) constitute a second group of autoimmune gastrointestinal, hepatobiliary and pancreatic illnesses. IgG4-related diseases are systemic and rare autoimmune illnesses. They often are connected with chronic inflammation and fibrotic reaction that can occur in any organ of the body. The most typical feature of these diseases is a mononuclear infiltrate with IgG4-positive plasma cells and self-sustaining inflammatory response. In this review, we focus especially upon the hepatopancreatobiliary system, autoimmune pancreatitis and IgG4-related sclerosing cholangitis. The cooperation of the gastroenterologist, radiologist, surgeon and histopathologist is crucial for establishing correct diagnoses and appropriate treatment, especially in IgG4 hepatopancreatobiliary diseases.
Omentin Is Independently Associated with Stroke Severity and Ipsilateral Carotid Artery Stenosis in Patients with Acute Cerebral Ischemia
Authors: Chondrogianni Maria , Lambadiari Vaia , Katsanos Aristeidis H. , Stefanou Maria Ioanna , Palaiodimou Lina , Triantafyllou Alexandros Stavros , Karagiannis Georgios , Konstantakos Vasileios , Ioakeimidis Michael , Triantafyllou Sokratis , Zompola Christina , Liantinioti Chryssa , Pappa Alexandra , Rizos Ioannis , Voumvourakis Konstantinos , Tsivgoulis Georgios , Boutati Eleni
Published: December 2021

Mounting evidence indicates an association between adipokines and inflammation-related atherosclerosis. Here, we sought to investigate the association of vaspin and omentin with clinical characteristics and outcomes of patients with acute cerebral ischemia (ACI). Consecutive ACI patients were evaluated within 24 h from symptom-onset. Stroke aetiology was classified using TOAST criteria. Adipokines were assayed using quantikine enzyme immunoassay commercially available kits. Stroke severity was assessed by NIHSS-score, and ipsilateral carotid stenosis (≥50% by NASCET criteria) by ultrasound and CT/MR angiography. Major cerebrovascular events were assessed at three months. We included 135 ACI patients (05 (78%) and 30 (22%) with acute ischemic stroke and transient ischemic attack, respectively; mean age ± SD: 59 ± 10 years; 68% men; median NIHSS-score: 3 (IQR:1–7)). Omentin was strongly correlated to admission stroke severity (Spearman rho coefficient: +0.303; p < 0.001). Patients with ipsilateral carotid stenosis had higher omentin levels compared to patients without stenosis (13.3 ± 8.9 ng/mL vs. 9.5 ± 5.5 ng/mL, p = 0.014). Increasing omentin levels were independently associated with higher stroke severity (linear regression coefficient = 0.290; 95%CI: 0.063–0.516; p = 0.002) and ipsilateral carotid stenosis (linear regression coefficient = 3.411; 95%CI: 0.194–6.628; p = 0.038). No association of vaspin with clinical characteristics and outcomes was found. Circulating omentin may represent a biomarker for the presence of atherosclerotic plaque, associated with higher stroke severity in ACI patients.
Associations between Early Surgery and Postoperative Outcomes in Elderly Patients with Distal Femur Fracture: A Retrospective Cohort Study
Authors: Yamamoto Norio , Ohbe Hiroyuki , Tomita Yosuke , Yorifuji Takashi , Nakajima Mikio , Sasabuchi Yusuke , Miyamoto Yuki , Matsui Hiroki , Noda Tomoyuki , Yasunaga Hideo
Published: December 2021

Previous literature has provided conflicting results regarding the associations between early surgery and postoperative outcomes in elderly patients with distal femur fractures. Using data from the Japanese Diagnosis Procedure Combination inpatient database from April 2014 to March 2019, we identified elderly patients who underwent surgery for distal femur fracture within two days of hospital admission (early surgery group) or at three or more days after hospital admission (delayed surgery group). Of 9678 eligible patients, 1384 (14.3%) were assigned to the early surgery group. One-to-one propensity score matched analyses showed no significant difference in 30-day mortality between the early and delayed groups (0.5% versus 0.5%; risk difference, 0.0%; 95% confidence interval, −0.7% to 0.7%). Patients in the early surgery group had significantly lower proportions of the composite outcome (death or postoperative complications), shorter hospital stays, and lower total hospitalization costs than patients in the delayed surgery group. Our results showed that early surgery within two days of hospital admission for geriatric distal femur fracture was not associated with a reduction in 30-day mortality but was associated with reductions in postoperative complications and total hospitalization costs.
Effect of Intra-Articular Injection of Platelet-Rich Plasma on the Serum Levels of Osteoarthritic Biomarkers in Patients with Unilateral Knee Osteoarthritis
Authors: Lacko Marek , Harvanová Denisa , Slovinská Lucia , Matuška Martin , Balog Marek , Lacková Antónia , Špaková Timea , Rosocha Ján
Published: December 2021

Background: The aim of this study is to determine the effect of three doses of intra-articular injection of platelet-rich plasma (PRP) into the osteoarthritic (OA) knee joint on the functional status and on the changes in the levels of specific OA biomarkers in blood serum. Methods: Forty patients with unilateral primary knee osteoarthritis were enrolled in this single center, prospective clinical trial. For each patient, three intra-articular PRP injections were administered one week apart. Clinical and laboratory assessment was performed before the first PRP injection (baseline), and 3 months after the third PRP application (3-month follow up). Pain in the affected knee joint was assessed with the Visual Analog Scale for Pain (VAS). Change in clinical status was evaluated with the Western Ontario and McMaster Universities Arthritis Index Questionnaire (WOMAC). Concentrations of 19 biomarkers (EGF, Eotaxin, FGF-2, GRO, IL-10, IL-1RA, IL-8, IP-10, MCP-1, PDGF-AB/BB, RANTES, MMP-3, MMP-13, Collagen type 2, BMP-2, TIMP-1, TIMP-2, TGF beta 1, and COMP) in the serum of studied patients were quantified. Results: At 3-month follow up, there was a significant decrease in the VAS score and significant improvement in the WOMAC score. There was a significant decrease in the levels of Eotaxin, MCP-1, MMP-1, IL-10, EGF, PDGF-AB/BB, TGF- β1 compared to baseline levels. A significant increase in markers BMP-2, COMP, Collagen type 2 and GRO was found at the same time point. There was no significant change in the concentrations of other biomarkers (FGF-2, IL-1RA, IL-8, IL-10, MMP-3, RANTES, TIMP-1, TIMP-3). Conclusions: We found an increase in specific pro-anabolic and anti-inflammatory biomarkers with a concomitant decrease in pro-inflammatory biomarkers at 3 months after three intra-articular applications of PRP. Significant improvement in VAS and WOMAC scores was observed. Treatment with PRP may be an effective therapeutic option with anti-inflammatory and regenerative potential in patients with primary knee OA.
Is It Possible to Predict Clonal Thrombocytosis in Triple-Negative Patients with Isolated Thrombocytosis Based Only on Clinical or Blood Findings?
Authors: Belčič Mikič Tanja , Vratanar Bor , Pajič Tadej , Anžej Doma Saša , Debeljak Nataša , Preložnik Zupan Irena , Sever Matjaž , Zver Samo
Published: December 2021

JAK2, MPL, and CALR mutations define clonal thrombocytosis in about 90% of patients with sustained isolated thrombocytosis. In the remainder of patients (triple-negative patients) diagnosing clonal thrombocytosis is especially difficult due to the different underlying conditions and possible inconclusive bone marrow biopsy results. The ability to predict patients with sustained isolated thrombocytosis with a potential clonal origin has a prognostic value and warrants further examination. The aim of our study was to define a non-invasive clinical or blood parameter that could help predict clonal thrombocytosis in triple-negative patients. We studied 237 JAK2 V617-negative patients who were diagnosed with isolated thrombocytosis and referred to the haematology service. Sixteen routine clinical and blood parameters were included in the logistic regression model which was used to predict the type of thrombocytosis (reactive/clonal). Platelet count and lactate dehydrogenase (LDH) were the only statistically significant predictors of clonal thrombocytosis. The platelet count threshold for the most accurate prediction of clonal or reactive thrombocytosis was 449 × 109/L. Other tested clinical and blood parameters were not statistically significant predictors of clonal thrombocytosis. The level of LDH was significantly higher in CALR-positive patients compared to CALR-negative patients. We did not identify any new clinical or blood parameters that could distinguish clonal from reactive thrombocytosis. When diagnosing clonal thrombocytosis triple-negative patients are most likely to be misdiagnosed. Treatment in patients with suspected triple negative clonal thrombocytosis should not be delayed if cardiovascular risk factors or pregnancy coexist, even in the absence of firm diagnostic criteria. In those cases the approach “better treat more than less” should be followed.
Shedding Light on Targeting Chronic Myeloid Leukemia Stem Cells
Authors: Houshmand Mohammad , Kazemi Alireza , Anjam Najmedini Ali , Ali Muhammad Shahzad , Gaidano Valentina , Cignetti Alessandro , Fava Carmen , Cilloni Daniela , Saglio Giuseppe , Circosta Paola
Published: December 2021

Chronic myeloid leukemia stem cells (CML LSCs) are a rare and quiescent population that are resistant to tyrosine kinase inhibitors (TKI). When TKI therapy is discontinued in CML patients in deep, sustained and apparently stable molecular remission, these cells in approximately half of the cases restart to grow, resuming the leukemic process. The elimination of these TKI resistant leukemic stem cells is therefore an essential step in increasing the percentage of those patients who can reach a successful long-term treatment free remission (TFR). The understanding of the biology of the LSCs and the identification of the differences, phenotypic and/or metabolic, that could eventually allow them to be distinguished from the normal hematopoietic stem cells (HSCs) are therefore important steps in designing strategies to target LSCs in a rather selective way, sparing the normal counterparts.
Prognostic Implications of a Modified Seattle Heart Failure Model Score Following Transcatheter Aortic Valve Replacement
Authors: Imamura Teruhiko , Narang Nikhil , Onoda Hiroshi , Tanaka Shuhei , Ushijima Ryuichi , Sobajima Mitsuo , Fukuda Nobuyuki , Ueno Hiroshi , Kinugawa Koichiro
Published: December 2021

Background: The Seattle heart failure model (SHFM) score is a well-known risk predictor of mortality in patients with heart failure. We validated this score in patients receiving transcatheter aortic valve replacement (TAVR) and aimed to generate further risk discrimination by adding invasive hemodynamics parameters. Methods: Patients who underwent TAVR at our institute between 2015 and 2020 were included and followed for 2 years from index discharge. Patients were randomly assigned to the derivation cohort or the validation cohort. In the derivation cohort, the original SHFM score was modified by adding baseline hemodynamics parameters to evaluate the primary outcomes: 2-year incidence of mortality or readmission from heart failure. The model performance of the modified SHFM score was evaluated in the validation cohort. Results: A total of 217 patients (median age: 86 (83, 88) years old, 64 (29%) men) were included. From the derivation cohort (N = 108), a novel modified SHFM score was constructed: 6 × (original SHFM score < 88.1%) + 5 × (pulmonary capillary wedge pressure > 14 mmHg) + 4 × (cardiac index < 2.26 L/min/m2), which had an improved discrimination compared with the original model (area under the curve: 0.887 vs. 0.679, p = 0.014). In the validation cohort (N = 109), the modified SHFM score showed accurate predictive discrimination of the 2-year cumulative incidence of the primary endpoint into three groups (a low score group with 0–5 points, 3%; an intermediate score group with 6–10 points, 12%; and a high score group with 11–15 points, 43%, p < 0.001). Conclusion: A modified SHFM score consisting of the original SHFM score and invasive hemodynamics parameters predicted mortality and morbidity following TAVR. Evaluation of the external validity of this score in other cohorts needs further investigation.
Analysis of Indications for Voiding Cystography in Children
Authors: Kopiczko Natalia , Dzik-Sawczuk Aleksandra , Szwarc Karolina , Czyż Anna , Wasilewska Anna
Published: December 2021

In this study, we report the experience of our center with the prognosis of vesicoureteral reflux, depending on the indications for voiding cystography, during a 12-year period. Retrospective analysis included 4302 children who were analyzed according to the indication for voiding cystography: (1) a febrile urinary tract infection, (2) urinary tract malformations on ultrasonography and (3) lower urinary tract dysfunction. Vesicoureteral reflux was found in 917 patients (21.32%; 24.1% of girls and 17.9% of boys). In group (1), reflux was found in 437/1849 cases (23.63%), group (2) in 324/1388 cases (23.34%) and group (3) in 156/1065 cases (14.65%). A significantly lower prevalence of reflux and its lower degree was found in children from group (3) when compared to other groups (p < 0.01). VURs were confirmed in over 20% of children with urinary tract malformations on ultrasonography or after a febrile urinary tract infection, suggesting the need for voiding cystography in these children. Indications for this examination in children with lower urinary tract dysfunction should be limited.
Rapid Targeted Method of Detecting Abused Piperazine Designer Drugs
Authors: Welz Anna , Koba Marcin , Kośliński Piotr , Siódmiak Joanna
Published: December 2021

Piperazine derivatives belong to the popular psychostimulating compounds from the group of designer drugs. They are an alternative to illegal drugs such as ecstasy and amphetamines. They are being searched by consumers for recreational use due to their stimulating and hallucinogenic effects. Many NPS-related poisonings and deaths have been reported where piperazines have been found. However, a major problem is the potential lack of laboratory confirmation of the involvement of piperazine derivatives in the occurrence of poisoning. Although many methods have been published, piperazine derivatives are not always included in a routine analytical approach or targeted toxicological analysis. There is an increasing need to provide qualitative evidence for the presence of piperazine derivatives and to ensure reproducible quantification. This article describes a new rapid method of detecting piperazine derivatives in biological material, using LC-MS. All target analytes were separated in a 15 min run time and identified based on the precursor ion, at least two product ions, and the retention time. Stable isotopically labeled (SIL) internal standards: BZP-D7, mCPP-D8 and TFMPP-D4 were used for analysis, obtaining the highest level of confidence in the results. The proposed detection method provides the analytical confirmation of poisoning with piperazine designer drugs.
Dehydration before Major Urological Surgery and the Perioperative Pattern of Plasma Creatinine: A Prospective Cohort Series
Authors: Löffel Lukas M. , Engel Dominique A. , Beilstein Christian M. , Hahn Robert G. , Furrer Marc A. , Wuethrich Patrick Y.
Published: December 2021

Preoperative dehydration is usually found in 30–50% of surgical patients, but the incidence is unknown in the urologic population. We determined the prevalence of preoperative dehydration in major elective urological surgery and studied its association with postoperative outcome, with special attention to plasma creatinine changes. We recruited 187 patients scheduled for major abdominal urological surgery to participate in a single-center study that used the fluid retention index (FRI), which is a composite index of four urinary biomarkers that correlate with renal water conservation, to assess the presence of dehydration. Secondary outcomes were postoperative nausea and vomiting (PONV), return of gastrointestinal function, in-hospital complications, quality of recovery, and plasma creatinine. The proportion of dehydrated patients at surgery was 20.4%. Dehydration did not correlate with quality of recovery, PONV, or other complications, but dehydrated patients showed later defecation (p = 0.02) and significant elevations of plasma creatinine after surgery. The elevations were also greater when plasma creatinine had increased rather than decreased during the 24 h prior to surgery (p < 0.001). Overall, the increase in plasma creatinine at 6 h after surgery correlated well with elevations on postoperative days one and two. In conclusion, we found preoperative dehydration in one-fifth of the patients. Dehydration was associated with delayed defecation and elevated postoperative plasma creatinine. The preoperative plasma creatinine pattern could independently forecast more pronounced increases during the early postoperative period.
Morphological and Phylogenetic Evidence for Recognition of Two New Species of Phanerochaete from East Asia
Authors: Wang Dong-Qiong , Zhao Chang-Lin
Published: December 2021

Two new corticioid fungal species, Phanerochaete pruinosa and P. rhizomorpha spp. nov. are proposed based on a combination of morphological features and molecular evidence. Phanerochaete pruinosa is characterized by the resupinate basidiomata with the pruinose hymenial surface, a monomitic hyphal system with simple-septate generative hyphae and subcylindrical basidiospores measuring as 3.5–6.7 × 1.5–2.7 µm. Phanerochaete rhizomorpha is characterized by having a smooth hymenophore covered by orange hymenial surface, the presence of rhizomorphs, subulate cystidia, and narrower ellipsoid to ellipsoid basidiospores. Sequences of ITS+nLSU nrRNA gene regions of the studied specimens were generated and phylogenetic analyses were performed with maximum likelihood, maximum parsimony, and Bayesian inference methods. These phylogenetic analyses showed that two new species clustered into genus Phanerochaete, in which P. pruinosa was sister to P. yunnanensis with high supports (100% BS, 100% BT, 1.00 BPP); morphologically differing by a pale orange to greyish orange and densely cracked hymenial surface. Another species P. rhizomorpha was closely grouped with P. citrinosanguinea with lower supports; morphologically having yellow to reddish yellow hymenial surface, and smaller cystidia measuring as 31–48 × 2.3–4.8 µm.
Review on Multiple Facets of Drug Resistance: A Rising Challenge in the 21st Century
Authors: Saha Mousumi , Sarkar Agniswar
Published: December 2021

With the advancements of science, antibiotics have emerged as an amazing gift to the human and animal healthcare sectors for the treatment of bacterial infections and other diseases. However, the evolution of new bacterial strains, along with excessive use and reckless consumption of antibiotics have led to the unfolding of antibiotic resistances to an excessive level. Multidrug resistance is a potential threat worldwide, and is escalating at an extremely high rate. Information related to drug resistance, and its regulation and control are still very little. To interpret the onset of antibiotic resistances, investigation on molecular analysis of resistance genes, their distribution and mechanisms are urgently required. Fine-tuned research and resistance profile regarding ESKAPE pathogen is also necessary along with other multidrug resistant bacteria. In the present scenario, the interaction of bacterial infections with SARS-CoV-2 is also crucial. Tracking and in-silico analysis of various resistance mechanisms or gene/s are crucial for overcoming the problem, and thus, the maintenance of relevant databases and wise use of antibiotics should be promoted. Creating awareness of this critical situation among individuals at every level is important to strengthen the fight against this fast-growing calamity. The review aimed to provide detailed information on antibiotic resistance, its regulatory molecular mechanisms responsible for the resistance, and other relevant information. In this article, we tried to focus on the correlation between antimicrobial resistance and the COVID-19 pandemic. This study will help in developing new interventions, potential approaches, and strategies to handle the complexity of antibiotic resistance and prevent the incidences of life-threatening infections.
The Relationship between LGBT Executives and Firms’ Value and Financial Performance
Authors: Lourenço Isabel Costa , Di Marco Donatella , Branco Manuel Castelo , Lopes Ana Isabel , Sarquis Raquel Wille , Soliman Mark T.
Published: December 2021

Drawing on resource-based theory, we analyze the relationship between having LGBT executives in a firm’s leadership positions and its value and financial performance. The existence of LGBT executives is considered to be associated with employee and customer goodwill towards LGBT-friendly policies and practices and to lead to human capital and reputational benefits. Our findings suggest that there is a positive effect of the presence of LBGT executives on a firm’s value, both directly and indirectly, through its effect on the firm’s financial performance. We interpret this as suggesting that besides the direct effect of the existence of LGBT executives on a firm’s value, an indirect effect also exists, mediated through financial performance, presumably through the effect that this has on employee and customer goodwill towards LGBT-friendly policies and practices. As far as we are aware, our study is the first to examine the impacts of the presence of LGBT executives, as well as distinguish between its direct and indirect effects on firm value.
Corporate Governance from a Cross-Country Perspective and a Comparison with Romania
Authors: Mihail Bogdan Aurelian , Dumitrescu Dalina
Published: December 2021

This paper investigates corporate governance from a cross-country perspective and makes a comparison with Romania. There are studies that examine the corporate governance issues related to Romanian companies, but these studies provide only qualitative and descriptive accounts of the research topic, with limited cross-country analysis. The present paper complements the literature by producing a quantitative analysis of cross-country corporate governance and makes a comparison with Romania. For this purpose, a set of corporate governance indicators from a large sample of 39 advanced and developing countries was collected for the 2006–2020 period. In terms of corporate governance dimensions, it was found that Romania underperforms other developing countries in the dimensions of director liability and ownership and control, while it outperforms them in the dimensions of corporate transparency, disclosure, and shareholder rights. The results indicate that the stagnant corporate governance scores and the low development level of stock markets stand out as important business challenges for the country. The correlation and regression analyses show that stock market development is closely associated with corporate governance dimensions and, overall, corporate governance scores matter greatly for the economic growth of countries, such as Romania, which can benefit greatly from the improvement of corporate governance codes and practices in the private sector.
Exploring the Moderating Role of Readers’ Perspective in Evaluations of Online Consumer Reviews
Authors: Abedin Ehsan , Mendoza Antonette , Karunasekera Shanika
Published: December 2021

Drawing upon the heuristic–systematic model (HSM) and considering the readers’ perspective, this study predicts that readers’ involvement and homophily between the reader and the review author (source) moderate the relationships between the credibility perception of online reviews and its antecedent factors. To test our hypotheses, we performed a user study on the Amazon Mechanical Turk platform. The results show that reader’s involvement moderates source credibility, internal consistency, review objectivity, and review sidedness on review credibility. In addition, homophily between the reader and the source also moderates the relationship between review credibility and its source. Our study contributes to information processing literature, especially in the context of online reviews, and suggests a better classification of the attributes related to online reviews using the HSM. Besides, it helps e-commerce platforms to customize online reviews for each reader to satisfy their information need and help them to make a better purchasing decision.
Of Animal Husbandry and Food Production—A First Step towards a Modular Agent-Based Modelling Platform for Socio-Ecological Dynamics
Authors: Günther Gerrit , Clemen Thomas , Duttmann Rainer , Schütt Brigitta , Knitter Daniel
Published: December 2021

Agent-based models provide detailed, bottom-up approaches to investigate complex socio-ecological systems. This study presents a first step towards a modular agent-based simulation that is based upon empirical data, as well as environmental suitability maps and an assessment of livestock units. To illustrate the capabilities of our simulation, we use a geographically explicit approach to simulate a component of the production of animal products of a rural settlement in the lower Bakırçay catchment, western Turkey. The model structurally couples various agent types representing several elements and processes of the animal husbandry and food production value chain, such as sedentary herders—practising daily, short-distance pastoralism—and their flocks of goats and sheep, as well as milking and slaughtering. The modelling tool captures the fundamental socio-ecological dynamics of animal husbandry and food production in rural settlements. Therefore, the tool is valuable as a basis to discuss hypotheses regarding the number of animals that are needed to cover the requirements of different growing populations.
Supporting Pro-Poor Reforms of Agricultural Systems in Eastern DRC (Africa) with Remotely Sensed Data: A Possible Contribution of Spatial Entropy to Interpret Land Management Practices
Authors: De Marinis Pietro , De Petris Samuele , Sarvia Filippo , Manfron Giacinto , Momo Evelyn Joan , Orusa Tommaso , Corvino Gianmarco , Sali Guido , Borgogno Enrico Mondino
Published: December 2021

In the eastern Democratic Republic of Congo, agriculture represents the most important economic sector, and land control can be considered a perpetual source of conflict. Knowledge of the existing production system distribution is fundamental for both informing national land tenure reforms and guiding more effective agricultural development interventions. The present paper focuses on existing agricultural production systems in Katoyi collectivity, Masisi territory, where returning Internally and Externally Displaced People are resettling. We aim to define a repeatable methodology for building evidence-based and updated knowledge concerning the spatial distribution of the two existing production systems: subsistence-oriented agriculture (SOA) and business-oriented agriculture (BOA). To this aim, we used a supervised object-based classification approach on remotely sensed Sentinel-2 imagery to classify land cover. To classify production systems further within the “agriculture” and “pasture” land use classes, binary classification based on an entropy value threshold was performed. An iterative approach was adopted to define the final HNDVI threshold that minimised commission and omission errors and maximised overall accuracy and class separability. The methodology achieved acceptable observed accuracy (OA equal to 80–90% respectively for agricultural and pasture areas) in the assessment. SOA and BOA respectively covered 24.4 and 75.6% of the collectivity area (34,606 ha). The results conclude that land use and entropy analysis can draw an updated picture of existing land distribution among different production systems, supporting better-adapted intervention strategies in development cooperation and pro-poor agrarian land tenure reforms in conflict-ridden landscapes.
Predictive Modelling of Landslide Susceptibility in the Western Carpathian Flysch Zone
Authors: Barančoková Mária , Šošovička Matej , Barančok Peter , Barančok Peter
Published: December 2021

Landslides are the most common geodynamic phenomenon in Slovakia, and the most affected area is the northwestern part of the Kysuca River Basin, in the Western Carpathian flysch zone. In this paper, we evaluate the susceptibility of this region to landslides using logistic regression and random forest models. We selected 15 landslide conditioning factors as potential predictors of a dependent variable (landslide susceptibility). Classes of factors with too detailed divisions were reclassified into more general classes based on similarities of their characteristics. Association between the conditioning factors was measured by Cramer’s V and Spearman’s rank correlation coefficients. Models were trained on two types of datasets—balanced and stratified, and both their classification performance and probability calibration were evaluated using, among others, area under ROC curve (AUC), accuracy (Acc), and Brier score (BS) using 5-fold cross-validation. The random forest model outperformed the logistic regression model in all considered measures and achieved very good results on validation datasets with average values of AUCval=0.967, Accval=0.928, and BSval=0.079. The logistic regression model results also indicate the importance of assessing the calibration of predicted probabilities in landslide susceptibility modelling.
Knowledge Mapping Analysis of Transnational Agricultural Land Investment Research
Authors: Han Jing , Jiang Mengying , Zhang Xupeng , Lu Xinhai
Published: December 2021

With the expansion of the global transnational agricultural planting scale, research on transnational agricultural land investment is growing. In order to analyze the development context and basic characteristics of this research, and to discover the research hotspots and frontiers, this study used documentation and bibliometric methods to examine the achievements of it. The results show the following: (1) Transnational agricultural land investment research is mainly focused on the social sciences, development studies, economics, environmental sciences and geography. (2) The concentration of researchers in this research field is not high, and there is still a lack of authoritative researchers with high influence. The cooperation network has been initially formed between research institutions. Among them, universities and research institutes are the main institutions of transnational agricultural land investment research, but the degree of integration among the research teams is not high. (3) The evolution of the research theme of the field has experienced three stages—an embryonic stage, growth stage and stable stage—and the research content shows a trend of continuous divergence and deepening. (4) From 2005 to 2019, the research hotspots of the research focused on “Land Grabbing, Global Land, Africa, Investment”. At present, the emerging frontier research topics are “Indonesia, Livelihood, Trajectory and Sustainability”. With many years of development, the research has become an obvious "land" attribute, independent from traditional agricultural economic research, and the research topics are becoming more and more mature, refined and diversified. Transnational agricultural land investment research is attracting continuous attention from scholars in multiple disciplines and fields.
Green Urban (RE) Generation: A Research and Practice Methodology to Better Implement Green Urban Infrastructure Solutions
Authors: Laurenz Jon , Belausteguigoitia Jone , de la Fuente Ander , Roehr Daniel
Published: December 2021

Green Urban Infrastructure Solutions (GUIS) are becoming more and more popular globally. Recent research reveals the environmental benefits derived from GUIS as well as their contribution to climate change adaptation. However, the urgent need for GUIS in order to meet the Paris Agreement, has not translated into an easy implementation thereof. This paper proposes a circular design methodology (CDM) where the combination of research and practice contributes to minimize both current skepticism and barriers when implementing GUIS. It includes a community engagement process to better understand their sensitivity and build consensus on GUIS. Additionally, GUIS are implemented, in a series of pilot projects and specific research is applied to comprehend the environmental benefits derived from these GUIS. The paper argues that GUIS represent a significant opportunity to respond to climate change risks as well as to achieve other urban benefits; however, in order to overcome existing barriers and skepticism, the proposed CDM reaches for more consensual urban solutions and drives uptake and implementation of GUIS, contributing to move from pilot project to common practice.
A Prebiotic Ribosylation of Pyrimidine Nucleobases Enabled by Metal Cations and Clay Minerals
Authors: Chen Qian-Qian , Zhao Ze-Run , Wang Xiao
Published: December 2021

We report a prebiotically relevant solution to the N1-ribosylation of pyrimidine nucleobases, a well-known challenge to the RNA world hypothesis. We found that the presence of metal cations and clay minerals enable the previously unachievable direct ribosylation of uracil. Spectroscopy and chromatography analyses confirmed the formation of ribosylated uracil. The method can be extended to the ribosylation of 2-pyrimidinone. These findings are also compatible with the metal-doped-clay model, developed by our lab for the unified route of the selection of ribose and subsequent syntheses of nucleotide and RNA.
Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients
Authors: Erfan Randa , Shaker Olfat G. , Khalil Mahmoud A. F. , Elsabagh Yumn A. , Ahmed Azza M. , Abu-El-Azayem Abeer K. , Gomaa Mohamed S. , Mohammed Asmaa
Published: December 2021

Objective: Long non-coding RNAs (lncRNAs) and their target microRNAs were documented in multiple studies to have a significant role in different joint disorders such as rheumatoid arthritis (RA) and osteoarthritis (OA). The current work aimed to determine the potential role of lnc-PVT1 and miR-146a as promising biomarkers to distinguish between RA, OA patients, and healthy individuals. Methods: The expression levels of lnc-PVT1 and its target miR-146a in the serum were measured for three different groups, including patients with RA (40), OA patients (40), and healthy controls (HCs) (40). Participating individuals were subjected to a full history investigation and clinical examination. Blood samples were tested for ESR, RF, CBC, as well as liver and renal functions. Serum was used to detect the relative expression levels of lnc-PVT1 and miR-146a and we correlated the levels with RA and OA activity and severity signs. Results: Lnc-PVT1 expression level was greater among patients with RA compared to that of OA patients, with a fold change median of 2.62 and 0.22, respectively (p = 0.001). The miR-146a fold change was significantly demonstrated between the RA, OA, and HCs groups. There was no correlation between both biomarkers with the disease activity scales (DAS28) of RA, the Knee injury Osteoarthritis Outcome Score (KOOS), or any sign of detection of the disease severity of OA. Conclusions: lnc-PVT1 and miR-146a could be considered as promising biomarkers for the diagnosis of RA and OA and may have an important role as therapeutic targets in the future.
A Comprehensive Framework to Evaluate the Effects of Anterior Cruciate Ligament Injury and Reconstruction on Graft and Cartilage Status through the Analysis of MRI T2 Relaxation Time and Knee Laxity: A Pilot Study
Authors: Marchiori Gregorio , Cassiolas Giorgio , Berni Matteo , Grassi Alberto , Dal Fabbro Giacomo , Fini Milena , Filardo Giuseppe , Zaffagnini Stefano , Lopomo Nicola Francesco
Published: December 2021

Background: Anterior cruciate ligament (ACL) tear represents a common orthopedic traumatic issue that often leads to an early development of osteoarthritis. To improve the diagnostic and prognostic techniques involved in the assessment of the joint after the trauma and during the healing process, the present work proposes a multi-parametric approach that aims to investigate the relationship between joint function and soft tissue status before and after ACL reconstruction. Methods: Thirteen consecutive patients who underwent ACL reconstruction were preliminarily enrolled in this study. Joint laxity assessment as well as magnetic resonance imaging with T2 mapping were performed in the pre-operative stage, at four and 18 months after surgery to acquire objective information to correlate knee function and soft tissue condition. Results: Correlations were found between graft and cartilage T2 signal, suggesting an interplay between these tissues within the knee joint. Moreover, graft maturation resulted in being connected to joint laxity, as underlined by the correlation between the graft T2 signal and the temporal evolution of knee function. Conclusions: This preliminary study represents a step forward in assessing the effects of ACL graft maturation on knee biomechanics, and vice versa. The presented integrated framework underlines the possibility to quantitatively assess the impact of ACL reconstruction on trauma recovery and cartilage homeostasis. Moreover, the reported findings—despite the preliminary nature of the clinical impacts—evidence the possibility of monitoring the surgery outcomes using a multi-parametric prognostic investigation tool.
Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib
Authors: Tchetina Elena V. , Satybaldyev Azamat M. , Markova Galina A. , Samarkina Elena Yu. , Lila Aleksandr M.
Published: December 2021

We investigated the importance of the baseline expression of genes involved in energy generation, as prognostic biomarkers of the treatment response to tofacitinib in patients with rheumatoid arthritis (RA). Peripheral blood samples were obtained from 28 patients with RA who received 3 months of tofacitinib therapy from 26 healthy controls. Clinical response was evaluated based on the disease activity score, the erythrocyte sedimentation rate (DAS28-ESR), and the serum levels of ACPA, RF, CRP, and ESR. Clinical remission was assessed based on DAS28 score <2.6. Protein concentrations were measured using ELISA. Total RNA isolated from whole blood was used for gene expression analysis using quantitative RT-PCR. All patients were diagnosed with Steinbrocker’s radiographic stage II-III at baseline, and most showed erosive arthritis with ACPA and RF positivity. Tofacitinib treatment significantly decreased the disease activity. Upon study completion, seven patients showed remission. Before and after TOFA therapy, a significantly higher expression of succinate dehydrogenase and pyruvate kinase genes was observed in all the examined patients compared to healthy subjects. However, the pre-therapy expression of these genes and corresponding proteins was significantly (p ≤ 0.05) lower in patients who showed remission than in other patients with RA. Moreover, we observed that, during follow-up, patients who developed remission showed an increasing trend in the expression of the examined genes, whereas the others showed some decreases in gene expression, although this was not statistically significant. We concluded that, compared with RA patients maintaining persistent moderate or high disease activity, those with clinical remission following tofacitinib treatment showed a significantly lower baseline expression of genes involved in energy generation.
Glutamate as a Stressoric Factor for the Ex Vivo Release of Catecholamines from the Rabbit Medial Prefrontal Cortex (mPFC)
Authors: Kania Bogdan Feliks , Wrońska Danuta , Szpręgiel Izabela , Bracha Urszula
Published: December 2021

One of the major roles of glutamic acid (Glu) is to serve as an excitatory neurotransmitter within the central nervous system (CNS). This amino acid influences the activity of several brain areas, including the thalamus, brainstem, spinal cord, basal ganglia, and pons. Catecholamines (CAs) are synthesized in the brain and adrenal medulla and by some sympathetic nerve fibers. CAs, including dopamine (DA), norepinephrine (NE), and epinephrine (E), are the principal neurotransmitters that mediate a variety of CNS functions, such as motor control, cognition, emotion, memory processing, pain, stress, and endocrine modulation. This study aims to investigate the effects of the application of various Glu concentrates (5, 50, and 200 µM) on CAs release from rabbit medial prefrontal cortex (mPFC) slices and compare any resulting correlations with CAs released from the hypothalamus during 90 min of incubation. Medial prefrontal cortex samples were dissected from decapitated, twelve-week-old female rabbits. The results demonstrated that Glu differentially influences the direct release of CAs from the mPFC and the indirect release of CAs from the hypothalamus. When under stress, the hypothalamus, a central brain structure of the HPA axis, induces and adapts such processes. Generally, there was an inhibitory effect of Glu on CAs release from mPFC slices. Our findings show that the effect arises from Glu’s action on higher-order motivational structures, which may indicate its contribution to the stress response by modulating the amount of CAs released.
Isolation and Characterization of Bovine RVA from Northeast China, 2017–2020
Authors: Cheng Xi , Wu Wei , Teng Fei , Yan Yue , Li Guiwei , Wang Li , Wang Xiaona , Wang Ruichong , Zhou Han , Jiang Yanping , Cui Wen , Tang Lijie , Li Yijing , Qiao Xinyuan
Published: December 2021

Group A rotaviruses (RVAs) are major enteric pathogens causing infections in calves. To investigate the epidemiological characteristics and genetic diversity of bovine rotavirus (BRV), 233 fecal samples were collected from calves with diarrhea in northeast China. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) and the outer capsid proteins VP7 and VP4 (G and P type, respectively) using RT-PCR. Ten of the 233 samples (4.3%) were identified as BRV positive and were used for virus isolation and sequence analysis, revealing that all strains analyzed were of the G6P[1] genotype. The isolates exhibited high VP6 sequence identity to the USA cow RVA NCDV strain (>99% amino acid identity) and were further shown to be closely related to Japanese cow RVA BRV101 and Israelian human RVA G6P[1] strains, with >99% amino acid identity to VP7 and VP4 proteins, respectively. Comparative analyses of genome-predicted amino acid sequences between the isolates and the NCDV strains indicated that the antigenicity and infectivity of the strains isolated had changed. In this study, BRV genotypes and the genetic diversity among vaccinated cattle herds were monitored to provide epidemiological data and references for early diagnosis, allowing for early detection of new, potentially pathogenic RVA strains.
Building Materials Made of Wood Waste a Solution to Achieve the Sustainable Development Goals
Authors: Maier Dorin
Published: December 2021

In order to reduce the impact of human activities on the environment, in 2015, the United Nations launched the 2030 Agenda for Sustainable Development, proposing 17 Sustainable Development Goals with 169 associated targets. It is well-known that the construction industry is a major contributor to global CO2 emissions, and if a solution to reduce construction activity is not possible, considering the increasing population, then other solutions must be developed to decrease their negative environmental impact. In this context, the purpose of this paper is to investigate whether the use of wood waste as a building material can be a solution to achieve the Sustainable Development Goals. The research procedure included a bibliometric literature search, a scientometric analysis and an in-depth discussion. The analysis was done with the help of the software VOSviewer and Bibliometrix; the data were extracted mainly from the ISI Web of Science database. The extraction of data was done using the PRISMA method, and thus a sample of 212 peer-reviewed journal articles was established. The main results indicate an increasing interest in this topic in the last several years, as well as a switch from considering wood waste as just a source to generate heat and energy to the use of wood waste as a building material. The main uses of wood waste as a building material are in the composition of particleboards and in various mortar and concrete mixtures. The field of wood waste has many potential directions towards future development, and if the immense treasure represented by the forests, and implicitly the wood, is used efficiently, it can be a good solution to the problem of sustainable development of society.
A Finite Element Analysis of the Effects of Graphene and Carbon Nanotubes on Thermal Conductivity of Co Phase in WC–Co Carbide
Authors: Li Zhengwu , Xiao Wenkai , Ruan Xuefeng
Published: December 2021

In engineering practice, the service life of cemented carbide shield tunneling machines in uneven soft and hard strata will be seriously reduced due to thermal stress. When carbon nanotubes (CNTs) and graphene nano-platelets (GNPs) are added to WC–Co carbide as enhanced phases, the thermal conductivity of carbide is significantly improved. Research should be performed to further understand the mechanism of enhancement in composites and to find ways to assist the design and optimization of the structure. In this paper, a series of finite element models were established using scripts to find the factors that affect the thermal conduction, including positions, orientations, interface thermal conductivity, shapes, sizes, and so on. WC–Co carbide with CNTs (0.06%, 0.12%, and 0.18% vol.), GNPs (0.06%, 0.12%, and 0.18% vol.) and hybrid CNTs–GNPs (1:1) were prepared to verify the reliability of finite element simulation results. The results show that the larger the interface thermal conductivity, the higher the composite phase thermal conductivity. Each 1%vol of CNTs increased the thermal conductivity of the composite phase by 7.2%, and each 1% vol. of GNPs increased the thermal conductivity of the composite phase by 5.2%. The proper curvature (around 140°) of CNTs and GNPs with a proper diameter to thickness ratio is suggested to lead to better thermal conductivity.