bluuebunny/crossref_metadata_2025 huggingface dataset:
Active Cell Divisions Generate Exotic Fourfold Orientationally Ordered Phase in Living Tissue
Authors: Cislo Dillon , Qin Haodong , Yang Fengshuo , Bowick Mark J. , Streichan Sebastian J.
Published: July 2021

Abstract

Morphogenesis, the process through which genes generate form, establishes tissue scale order as a template for constructing the complex shapes of the body plan. The extensive growth required to build these ordered substrates is fuelled by cell proliferation, which, naively, should destroy order. The active mechanisms that couple cellular and physical processes to generate and maintain global order, thereby reconciling this seeming contradiction, remain elusive. Using live imaging and tissue cartography, we quantitatively analyze the dynamics of fourfold tissue ordering in the crustaceanParhyale hawaiensis. We show that cell divisions are the main drivers of tissue flow leading to a fourfold orientationally ordered phase. Waves of anisotropic cell proliferation propagate across the embryo with precise choreography, such that defects introduced into the nascent lattice by cell divisions are healed by subsequent divisions through active defect climb. Orchestrating cell proliferation rates and orientations enables cell divisions to organize, rather than fluidize, the tissue. The result is a robust, active mechanism for generating global orientational order in a non-equilibrium system that sets the stage for the subsequent development of shape and form.
Host cell maturation modulates parasite invasion and sexual differentiation in Plasmodium
Authors: Hentzschel Franziska , Gibbins Matthew P , Attipa Charalampos , Beraldi Dario , Moxon Christopher A , Otto Thomas D , Marti Matthias
Published: July 2021

Malaria remains a global health problem with over 400,000 deaths annually1. Plasmodium parasites, the causative agents of malaria, replicate asexually in red blood cells (RBCs) of their vertebrate host, while a subset differentiates into sexual stages (gametocytes) for mosquito transmission. Parasite replication and gametocyte maturation in the erythropoietic niches of the bone marrow and spleen contribute to pathogenesis and drive transmission2, but the mechanisms underlying this organ enrichment remain unknown. We performed a comprehensive single cell analysis of rodent P. berghei in spleen, bone marrow and blood to define parasite phenotypes specific to those niches. Single cell RNA-seq analysis of host and parasite cells reveals an interferon-driven host response to infection as well as transcriptional adaptations of Plasmodium to RBC maturation status. We show that P. berghei exhibits a bimodal invasion pattern into either normocytes or early reticulocytes and, using functional assays, identify CD71 as a host receptor for reticulocyte invasion. Importantly, we observe an increased rate of gametocyte formation in reticulocytes that is nutrient-dependent and triggered post invasion (i.e., same cycle sexual commitment). Our data provides a thorough characterisation of host-parasite interactions in erythropoietic niches and defines host cell maturation state as the key driver of parasite adaptation.
Solution NMR and racemic crystallography provide insights into a novel structural class of cyclic plant peptides
Authors: Payne Colton D. , Vadlamani Grishma , Hajiaghaalipour Fatemeh , Muhammad Taj , Fisher Mark F. , Andersson Håkan S. , Göransson Ulf , Clark Richard J. , Bond Charles S. , Mylne Joshua S. , Rosengren K. Johan
Published: July 2021

Head-to-tail cyclic and disulfide-rich peptides are natural products with applications in drug design. Among these are the PawS-Derived Peptides (PDPs) produced in seeds of the daisy plant family. PDP-23 is a unique member of this class in that it is twice the typical size and adopts two β-hairpins separated by a hinge region. The β-hairpins - both stabilised by a single disulfide bond - fold together into a V-shaped tertiary structure creating a hydrophobic core. In water two PDP-23 molecules merge their hydrophobic cores to form a square prism quaternary structure. Here, we synthesised PDP-23 and its enantiomer comprising all D-amino acids, which allowed us to confirm these solution NMR structural data by racemic crystallography. Furthermore, we discovered the related PDP-24. NMR analysis showed that PDP-24 does not form a dimeric structure and it has poor water solubility, but in less polar solvents adopts near identical secondary and tertiary structure to PDP-23. The natural role of these peptides in plants remains enigmatic, as we did not observe any antimicrobial or insecticidal activity. However, the plasticity of these larger PDPs and their ability to change structure under different conditions make them appealing peptide drug scaffolds.
The iron maiden. Cytosolic aconitase/IRP1 conformational transition in the regulation of ferritin translation and iron hemostasis
Authors: Hognon Cécilia , Bignon Emmanuelle , Harle Guillaume , Touche Nadège , Grandemange Stéphanie , Monari Antonio
Published: July 2021

Maintaining iron homeostasis is fundamental for almost all living being, and its deregulation correlates with severe and debilitating pathologies. The process is made more complicated by the omnipresence of iron and by its role as a fundamental component of a number of crucial metallo proteins. The response to modifications in the amount of the free iron pool is performed via the inhibition of ferritin translation by sequestering consensus messenger RNA (mRNA) sequences. In turn this is regulated by the iron-sensitive conformational equilibrium between aconitase and IRP, mediated by the presence of an iron-sulfur cluster. In this contribution we analyze by full-atom molecular dynamics simulation, the factors leading to both the interaction with mRNA, and the conformational transition. Furthermore, the role of the iron-sulfur cluster in driving the conformational transition is assessed by obtaining the related free energy profile via enhanced sampling molecular dynamics simulations.
The contribution of uncharted RNA sequences to tumor identity in lung adenocarcinoma
Authors: Wang Yunfeng , Xue Haoliang , Aglave Marine , Lainé Antoine , Gallopin Mélina , Gautheret Daniel
Published: July 2021

Background

Transcriptome analysis of cancer tissues has been instrumental in defining tumor subtypes, diagnostic signatures and cancer regulatory networks. Cancer transcriptomes are still predominantly analyzed at the level of gene expression. Few studies have addressed transcript-level variations, and most of these only looked at splice variants. Previously we introduced a k-mer based, reference-free method, DE-kupl, that performs differential analysis of RNA-seq data at the k-mer level, which enables distinguishing RNAs differing by a single nucleotide. Here we evaluate the significance of differential events discovered by this method in two independent lung adenocarcinoma RNA-seq datasets (N=583 and N=154).

Results

Focusing on differential events in a tumor vs normal setting, we found events in endogenous repeats, alternative splicing and polyadenylation sites, long non-coding RNAs, retained introns and unmapped RNAs. Replicability was highly significant for most event classes (assessed by comparing to events shared between unrelated tumors). Overall about 160,000 differential k-mer contigs were shared between datasets, including a large set of sequences from hypervariable genes such as immunoglobulins,SFTPand mucin genes. Most interestingly, we identified a set of novel tumor-specific long non-coding RNAs in intergenic and intronic regions. We found that expressed endogenous transposons defined two major groups of patients (high/low repeat expression) with distinct clinical characteristic. A number of repeats, intronic RNAs and lincRNA achieved strong patient stratification in univariate or multivariate survival models. Finally, using antigen presentation prediction, we identified 55 contigs predicted to produce recurrent tumor-specific antigens.

Conclusions

K-mer based RNA-seq analysis enables description of cancer transcriptomes at nucleotide precision, independently of prior transcript annotation. Application to lung cancer data uncovered events stemming from a wide variety of transcriptional and postranscriptional mechanisms. Among those events, a significant subset was replicable between cohorts, thus constituting novel RNA hallmarks of cancer. The code is available at:https://github.com/Transipedia/dekupl-lung-cancer-inter-cohort.
Structural and functional network-level reorganization in the coding of auditory motion directions and sound source locations in the absence of vision
Authors: Battal Ceren , Gurtubay-Antolin Ane , Rezk Mohamed , Mattioni Stefania , Bertonati Giorgia , Occelli Valeria , Bottini Roberto , Targher Stefano , Maffei Chiara , Jovicich Jorge , Collignon Olivier
Published: July 2021

hMT+/V5 is a region in the middle occipito-temporal cortex that responds preferentially to visual motion in sighted people. In case of early visual deprivation, hMT+/V5 enhances its response to moving sounds. Whether hMT+/V5 contains information about motion directions and whether the functional enhancement observed in the blind is motion specific, or also involves sound source location, remains unsolved. Moreover, the impact of this crossmodal reorganization of hMT+/V5 on the regions typically supporting auditory motion processing, like the human Planum Temporale (hPT), remains equivocal. We used a combined functional and diffusion MRI approach and individual in-ear recordings to study the impact of early blindness on the brain networks supporting spatial hearing, in male and female humans. Whole-brain univariate analysis revealed that the anterior portion of hMT+/V5 responded to moving sounds in sighted and blind people, while the posterior portion was selective to moving sounds only in blind participants. Multivariate decoding analysis revealed that the presence of motion directions and sound positions information was higher in hMT+/V5 and lower in hPT in the blind group. While both groups showed axis-of-motion organization in hMT+/V5 and hPT, this organization was reduced in the hPT of blind people. Diffusion MRI revealed that the strength of hMT+/V5 – hPT connectivity did not differ between groups, whereas the microstructure of the connections was altered by blindness. Our results suggest that the axis-of-motion organization of hMT+/V5 does not depend on visual experience, but that blindness alters the response properties of occipito-temporal networks supporting spatial hearing in the sighted.

Significance Statement

Spatial hearing helps living organisms navigate their environment. This is certainly even more true in people born blind. How does blindness affect the brain network supporting auditory motion and sound source location? Our results show that the presence of motion directions and sound positions information was higher in hMT+/V5 and lower in hPT in blind relative to sighted people; and that this functional reorganization is accompanied by microstructural (but not macrostructural) alterations in their connections. These findings suggest that blindness alters crossmodal responses between connected areas that share the same computational goals.
Large-scale genome-wide association study of food liking reveals genetic determinants and genetic correlations with distinct neurophysiological traits
Authors: May-Wilson Sebastian , Matoba Nana , Wade Kaitlin , Hottenga Jouke-Jan , Concas Maria Pina , Mangino Massimo , Grzeszkowiak Eryk J. , Menni Cristina , Gasparini Paolo , Timpson Nicholas J. , Veldhuizen Maria G. , de Geus Eco , Wilson James F. , Pirastu Nicola
Published: July 2021

Variable preferences for different foods are among the main determinants of their intake and are influenced by many factors, including genetics. Despite considerable twins’ heritability, studies aimed at uncovering food-liking genetics have focused mostly on taste receptors. Here, we present the first results of a large-scale genome-wide association study of food liking conducted on 161,625 participants from UK Biobank. Liking was assessed over 139 specific foods using a 9-point hedonic scale. After performing GWAS, we used genetic correlations coupled with structural equation modelling to create a multi-level hierarchical map of food liking. We identified three main dimensions: high caloric foods defined as “Highly palatable”, strong-tasting foods ranging from alcohol to pungent vegetables, defined as “Learned” and finally “Low caloric” foods such as fruit and vegetables. The “Highly palatable” dimension was genetically uncorrelated from the other two, suggesting that two independent processes underlie liking high reward foods and the Learned/Low caloric ones. Genetic correlation analysis with the corresponding food consumption traits revealed a high correlation, while liking showed twice the heritability compared to consumption. For example, fresh fruit liking and consumption showed a genetic correlation of 0.7 with heritabilities of 0.1 and 0.05, respectively. GWAS analysis identified 1401 significant food-liking associations located in 173 genomic loci, with only 11 near taste or olfactory receptors. Genetic correlation with morphological and functional brain data (33,224 UKB participants) uncovers associations of the three food-liking dimensions with non-overlapping, distinct brain areas and networks, suggestive of separate neural mechanisms underlying the liking dimensions. In conclusion, we created a comprehensive and data-driven map of the genetic determinants and associated neurophysiological factors of food liking beyond taste receptor genes.
Rapid redistribution and extensive binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates
Authors: Thompson Joyce J. , Lee Daniel J. , Mitra Apratim , Frail Sarah , Dale Ryan , Rocha Pedro P.
Published: July 2021

Establishment of divergent cell types from a common progenitor requires transcription factors (TFs) to promote lineage-restricted transcriptional programs while suppressing alternative fates. In the mouse blastocyst, cells of the inner cell mass (ICM) coexpress NANOG and GATA6, two TFs that drive the bifurcation of these progenitors into either the epiblast (Epi) or the primitive endoderm (PrE), respectively. Here, using in vitro differentiation, we describe the molecular mechanisms of how GATA6 quickly induces the PrE fate while repressing the Epi lineage. GATA6 functions as a pioneer TF by inducing nucleosome repositioning at regulatory elements controlling PrE genes, making them accessible for deposition of active histone marks and leading to rewiring of chromatin interactions and ultimately transcriptional activation. GATA6 also binds most regulatory elements of Epi genes followed by eviction of the Epispecific TFs NANOG and SOX2, loss of active histone marks, and reduction in chromatin accessibility that culminates in transcriptional repression. Unexpectedly, evicted NANOG and SOX2 transiently bind PrE regulatory elements occupied by GATA6. Our study shows that GATA6 binds and modulate the same regulatory elements as Epi TFs, a phenomenon we also validated in blastocysts. We propose that the ability of PrE and Epi-specific TFs to extensively bind and regulate the same gene networks contributes to ICM plasticity and allows rapid cell lineage specification by coordinating both activation and repression of divergent transcriptional programs.
Evolution of Regulatory Complexity for Cell-Cycle Control
Authors: von der Dunk Samuel H. A. , Snel Berend , Hogeweg Paulien
Published: July 2021

How complexity arises is a fundamental evolutionary question. Complex gene regulation is thought to arise by the interplay between adaptive and non-adaptive forces at multiple organizational levels. Using a computational model, we investigate how complexity arises in cell-cycle regulation. Starting from the well-known Caulobacter crescentus network, we study how cells adapt their cell-cycle behaviour to a gradient of limited nutrient conditions using 10 replicate in silico evolution experiments.

We find adaptive expansion of the gene regulatory network: improvement of cell-cycle behaviour allows cells to overcome the inherent cost of complexity. Replicates traverse different evolutionary trajectories leading to distinct eco-evolutionary strategies. In four replicates, cells evolve a generalist strategy to cope with a variety of nutrient levels; in two replicates, different specialist cells evolve for specific nutrient levels; in the remaining four replicates, an intermediate strategy evolves. The generalist and specialist strategies are contingent on the regulatory mechanisms that arise early in evolution, but they are not directly linked to network expansion and overall fitness.

This study shows that functionality of cells depends on the combination of gene regulatory network topology and genome structure. For example, the positions of dosage-sensitive genes are exploited to signal to the regulatory network when replication is completed, forming a de novo evolved cell-cycle checkpoint. Complex gene regulation can arise adaptively both from expansion of the regulatory network and from the genomic organization of the elements in this network, demonstrating that to understand complex gene regulation and its evolution, it is necessary to integrate systems that are often studied separately.
Elevated fear responses to threatening cues in rats with early life stress is associated with greater excitability and loss of gamma oscillations in ventral-medial prefrontal cortex
Authors: Bercum Florencia M , Navarro Gomez Maria J , Saddoris Michael P
Published: July 2021

Stress experienced early in development can have profound influences on developmental trajectories and ultimately behaviors in adulthood. Potent stressors during brain maturation can profoundly disrupt prefrontal cortical areas in particular, which can set the stage for prefrontal-dependent alterations in fear regulation and risk of drug abuse in adulthood. Despite these observations, few studies have investigated in vivo signaling in prefrontal signals in animals with a history of early life stress (ELS). Here, rats with ELS experienced during the first post-natal week were then tested on a conditioned suppression paradigm during adulthood. During conditioned suppression, electrophysiological recordings were made in the ventral medial prefrontal cortex (vmPFC) during presentations of a fear-associated cues that resolved both single-unit activity and local field potentials (LFPs). Relative to unstressed controls, ELS-experienced rats showed greater fear-related suppression of lever pressing. During presentations of the fear-associated cue (CS+), neurons in the vmPFC of ELS animals showed a significant increase in the probability of excitatory encoding relative to controls, and excitatory phasic responses in the ELS animals were reliably of higher magnitude than Controls. In contrast, vmPFC neurons in ELS subjects better discriminated between the shock-associated CS+ and the neutral (“safe”) CS-cue than Controls. LFPs recorded in the same locations revealed that high gamma band (65-95 Hz) oscillations were strongly potentiated in Controls during presentation of the fear-associated CS+ cue, but this potentiation was abolished in ELS subjects. Notably, no other LFP spectra differed between ELS and Controls for either the CS+ or CS-. Collectively, these data suggest that ELS experience alters the neurobehavioral functions of PFC in adulthood that are critical for processing fear regulation. As such, these alterations may also provide insight into to increased susceptibility to other PFC-dependent processes such as risk-based choice, motivation, and regulation of drug use and relapse in ELS populations.
The identification and structural analysis of potential 14-3-3 interaction sites on the bone regulator protein Schnurri-3
Authors: Soini Lorenzo , Leysen Seppe , Crabbe Tom , Davis Jeremy , Ottmann Christian
Published: July 2021

14-3-3 proteins regulate many intracellular processes and their ability to bind in subtly different fashions to their numerous partner proteins provides attractive drug-targeting points for a range of diseases. Schnurri-3 is a suppressor of mouse bone formation and a candidate target for novel osteoporosis therapeutics, and thus it is of interest to determine whether it interacts with 14-3-3. In this work, potential 14-3-3 interaction sites on mammalian Schnurri-3 were identified by an in silico analysis of its protein sequence. Using fluorescence polarization, isothermal titration calorimetry and X-ray crystallography, it is shown that synthetic peptides containing either phosphorylated Thr869 or Ser542 can indeed interact with 14-3-3, with the latter capable of forming an interprotein disulfide bond with 14-3-3σ: a hitherto unreported phenomenon.
Crystal structure of the TLDc domain of human NCOA7-AS
Authors: Arnaud-Arnould Mary , Tauziet Marine , Moncorgé Olivier , Goujon Caroline , Blaise Mickaël
Published: July 2021

The TLDc [Tre2/Bub2/Cdc16 (TBC), lysin motif (LysM), domain catalytic] domain is associated with oxidation-resistance related functions and is well conserved among eukaryotes. Seven proteins possess a TLDc domain in humans, notably proteins belonging to the oxidation resistance protein (OXR), nuclear receptor coactivator 7 (NCOA7) and TBC1 domain family member 24 (TBC1D24) families. Although the mechanism is unknown, a protective role of TLDc proteins against oxidative stress, notably in the brain, has been demonstrated. Neurobiological disorders caused by mutations in the TLDc domain have also been reported. The human NCOA7 gene encodes several mRNA isoforms; among these, isoform 4, named NCOA7-AS, is up-regulated by type 1 interferon in response to viral infection. NCOA7 and NCOA7-AS both interact with several subunits of the vacuolar proton pump V-ATPase, which leads to increased acidification of the endolysosomal system and consequently impairs infection by viruses that enter their host cells through the endosomal pathway, such as influenza A virus and hepatitis C virus. Similarly to full-length NCOA7, NCOA7-AS possesses a TLDc domain in its C-terminus. Structures of TLDc domains have been reported from zebrafish and fly but not from humans. Here, the expression, purification and crystallization of the TLDc domain from NCOA7 and NCOA7-AS is reported. The crystal structure solved at 1.8 Å resolution is compared with previously solved three-dimensional structures of TLDc domains.
The X-ray structure ofL-threonine dehydrogenase from the common hospital pathogenClostridium difficile
Authors: Adjogatse Eyram , Bennett Josh , Guo Jingxu , Erskine Peter T. , Wood Steve P. , Wren Brendan W. , Cooper Jonathan B.
Published: July 2021

In many prokaryotes, the first step of threonine metabolism is catalysed by the enzyme threonine dehydrogenase (TDH), which uses NAD+to oxidize its substrate to 2-amino-3-ketobutyrate. The absence of a functional TDH gene in humans suggests that inhibitors of this enzyme may have therapeutic potential against pathogens which are reliant on this enzyme. Here, TDH fromClostridium difficilehas been cloned and overexpressed, and the X-ray structure of the apoenzyme form has been determined at 2.6 Å resolution.
Minimally processed versus processed and ultra-processed food in individuals at cardiometabolic risk
Authors: Silva Meneguelli Talitha , Juvanhol Leidjaira Lopes , da Silva Leite Adriana , Bressan Josefina , Hermsdorff Helen Hermana Miranda
Published: July 2021

Purpose

The purpose of this paper is to evaluate the association between food consumption classified by the degree of processing and cardiometabolic risk factors in a population at risk of cardiovascular disease.

Design/methodology/approach

A cross-sectional study conducted with 325 adults and elderlies who present a cardiovascular risk factor. The food consumption was evaluated by a 24 h dietary recall, and it was classified according to the NOVA classification.

Findings

Individuals who presented a higher consumption of processed and ultra-processed food had a higher prevalence of abdominal obesity, waist/hip ratio (PR = 1.005; p-value = 0.049), waist circumference (PR = 1.003; p-value = 0.02) and high total cholesterol (PR = 1.008; p-value = 0.047), while ultra-processed had a higher prevalence of excess weight (PR = 1.004; p-value = 0.04), and abdominal obesity, waist/hip ratio (PR = 1.005; p-value = 0.04), waist circumference (PR = 1.004; p-value = 0.004) and waist/height ratio (PR = 1.003; p-value = 0.03).

Practical implications

An association was found between the degree of food processing and cardiometabolic risk factors, even in a population that already has a risk factor for cardiovascular disease, reinforcing the importance of personalized nutrition orientation that considers the profile of the target population as well as types of meals.

Originality/value

Food processing in itself can influence cardiometabolic risk and, as far as is known, no study has evaluated food processing in individuals who already have some type of cardiovascular risk. Also, consumption was assessed by the degree of processing between meals.
Does capacity utilisation influence financial performance? A study of Indian public bus transport companies
Authors: Kushwaha Shivam , Prawesh Shankar , Venkatesh Anand
Published: July 2021

Purpose

The objective of the paper is to get a better understanding of capacity utilisation (CU) in Indian public bus companies. More specifically, this paper would be measuring CU and identifying the drivers of the same. Finally, the influence of CU on the financial performance of Indian bus companies is examined.

Design/methodology/approach

The study adopted data envelopment analysis (DEA) to measure the CU in Indian public bus companies. Truncated regression was used to identify the drivers of CU. Subsequently, the ordinary least squares (OLS) regression was used to analyse the influence of CU on Indian bus companies' financial performance. The period of study was from 2013 to 17.

Findings

The significant drivers of CU were fleet age, passenger lead and fleet utilisation. Additionally, it was found that CU had a significant positive influence on the financial performance of Indian public bus companies and a unit increase in unused capacity has led to an increase of 7% in the operating ratio of the bus companies.

Practical implications

Getting insights into CU, apart from technical efficiency, is of immense use to both public transport researchers and practitioners. Managers of public bus companies should be mindful of CU as it has a significant bearing on their financial performance.

Originality/value

This is the first study in public transport, which establishes the linkage between CU and financial performance. Besides, a modified measure of cost-efficiency has also been conceptualised in this study.
Learning language and gaining employment: problems for refugee migrants in Australia
Authors: Hsieh Yi-Jung Teresa
Published: July 2021

Purpose

Muslim refugee migrants are a growing ethno-religious disadvantaged minority group in several Western societies, and host-country language proficiency and employment are essential factors in reducing this disadvantage. This paper thus explores the efficacy of English training programs in facilitating the settlement and employment of a group of male Muslim refugees in Australia.

Design/methodology/approach

This study is qualitative in nature, with data collected using semi-structured, in-depth interviews with the eight participants in the study. Analysis was conducted using Bourdieu's concepts of field, capital and habitus.

Findings

English training programs offered to Australian Muslim men are problematic in their aim of linking them to employment. Areas of concern are identified in respect to the training hours offered, their learning environment, their content and pedagogy, their lack of focus on employment and their failure to recognise the existing work skills of the migrants.

Research limitations/implications

The study is conducted with a small sample of male Muslim migrants: while the findings may be similar for other refugee groups, further research is necessary to confirm this.

Practical implications

There is a need to restructure the current English training programs offered to refugee migrants in Australia, Muslim or otherwise. This study identifies several areas where such restructuring might occur, both at the policy and pedagogical levels.

Originality/value

Few studies focus on Australian male Muslim migrants. This study enhances understanding of this under-researched group and their struggles to learn English, find employment and rise above their disadvantaged societal position.
A “crescendo” model: designing food experiences for psychological well-being
Authors: Zarantonello Lia , Grappi Silvia , Formisano Marcello , Schmitt Bernd H.
Published: July 2021

Purpose

This paper aims to advance the design-thinking approach in food from an engineering mind-set toward a positive psychology perspective by investigating how consumer experiences evoked by food-related activities can facilitate, stimulate and enhance individuals’ happiness and perceptions of life satisfaction.

Design/methodology/approach

A diary field experiment was conducted. Participants from a major European city were asked to reflect on their food-related activities, provide descriptions and answer questions on experiential stimulation derived from these activities in relation to happiness and perceived life satisfaction.

Findings

Food-related activities generally result in positive consumer experiences and psychological well-being. Experiential stimulation resulting from food activities is positively related to perceived life satisfaction directly and indirectly via pleasure and meaning. Although the authors found an overall positive relationship between these constructs, they also found differences based on the experience type considered. A “crescendo model” of experiences that details how experiences lead to happiness and perceived life satisfaction is presented.

Research limitations/implications

This study is largely exploratory. Future research should adopt an experimental approach and further test the relationship between experiential stimulation, happiness and perceived life satisfaction in the context of food.

Practical implications

The paper offers innovation teams in food companies a practical “crescendo model” that can be used to design product–consumer interactions.

Originality/value

The research bridges literatures on design thinking, psychological well-being and consumer experiences. By studying the relationship between experiences, happiness and perceived life satisfaction in the context of food, the findings contribute to research on food well-being by expanding the notion of happiness seen only as pleasure. The research also contributes to work on design thinking by offering an experiential framework that contributes to the notion of consumer empathy.
The impact of linguistic style on customer satisfaction: a cross-cultural empirical study in Ecuador
Authors: Fan Alei , Van Hoof Hubert B. , Dou Xueting , Serrano Ana Lucia
Published: July 2021

Purpose

Drawing on the dual process theory and the cultural dimension of power distance, the current research investigates the impact of a specific service clue—the linguistic style of address forms (salutation) in hotel manager letters to guests—on customer satisfaction in a hotel context in Ecuador.

Design/methodology/approach

Following an experimental design research approach, this research conducted a series of two studies to examine how customers' cultural values (high vs low power distance), linguistic style of address forms (formal vs casual) and service valence (service success vs service failure) together influenced customer satisfaction. Specifically, Study 1 examined the service success condition, and Study 2 investigated the service failure condition.

Findings

The research results show that, in the service success condition, customers follow their distinct cultural orientations (high vs low power distance) when responding to the different linguistic styles (formal vs casual). On the other hand, in the service failure situation, as customers desire for expressions of respect that can be reflected in a formal address form, the level of satisfaction is lower when the casual address form is used in guest communications, regardless of customers' cultural orientations in power distance.

Originality/value

This research adds to existing cross-cultural service research, particularly in terms of service valence, and provides practical implications for enhancing service providers' cultural awareness and sociolinguistic competence to effectively communicate with customers from diverse cultural backgrounds.
Exploring how teachers suggest informing designs for learning
Authors: Hrastinski Stefan
Published: July 2021

Purpose

An issue that has received limited attention is how teachers make informed decisions when designing learning activities, which makes effective use of resources and technologies. The aim of this paper is to explore how teachers suggest informing their designs for learning.

Design/methodology/approach

The study is based on documentation from workshops where adult education teachers were encouraged to suggest how a design for learning can draw on what is known, how the design can be evaluated and how the design and lessons learned can be shared. The data was analyzed using thematic analysis.

Findings

Most teachers did not explicitly suggest how to inform their designs for learning, although some suggested to draw on learning theories. The teachers were able to provide many examples of how their designs for learning could be evaluated based on student perceptions and assessment. They primarily suggested to discuss and share their designs and lessons learned at the school.

Practical implications

The workshop format could be adapted and used in practice.

Originality/value

Relatively little attention has been paid to how teachers inform their designs for learning. A key challenge for teachers is to understand design for learning as an informed practice, which could be integrated as part of their daily work.
Artificial intelligence and human workers interaction at team level: a conceptual assessment of the challenges and potential HRM strategies
Authors: Arslan Ahmad , Cooper Cary , Khan Zaheer , Golgeci Ismail , Ali Imran
Published: July 2021

Purpose

This paper aims to specifically focus on the challenges that human resource management (HRM) leaders and departments in contemporary organisations face due to close interaction between artificial intelligence (AI) (primarily robots) and human workers especially at the team level. It further discusses important potential strategies, which can be useful to overcome these challenges based on a conceptual review of extant research.

Design/methodology/approach

The current paper undertakes a conceptual work where multiple streams of literature are integrated to present a rather holistic yet critical overview of the relationship between AI (particularly robots) and HRM in contemporary organisations.

Findings

We highlight that interaction and collaboration between human workers and robots is visible in a range of industries and organisational functions, where both are working as team members. This gives rise to unique challenges for HRM function in contemporary organisations where they need to address workers' fear of working with AI, especially in relation to future job loss and difficult dynamics associated with building trust between human workers and AI-enabled robots as team members. Along with these, human workers' task fulfilment expectations with their AI-enabled robot colleagues need to be carefully communicated and managed by HRM staff to maintain the collaborative spirit, as well as future performance evaluations of employees. The authors found that organisational support mechanisms such as facilitating environment, training opportunities and ensuring a viable technological competence level before organising human workers in teams with robots are important. Finally, we found that one of the toughest challenges for HRM relates to performance evaluation in teams where both humans and AI (including robots) work side by side. We referred to the lack of existing frameworks to guide HRM managers in this concern and stressed the possibility of taking insights from the computer gaming literature, where performance evaluation models have been developed to analyse humans and AI interactions while keeping the context and limitations of both in view.

Originality/value

Our paper is one of the few studies that go beyond a rather general or functional analysis of AI in the HRM context. It specifically focusses on the teamwork dimension, where human workers and AI-powered machines (robots) work together and offer insights and suggestions for such teams' smooth functioning.
Who cares about ethical practices at workplace? A taxonomy of employees’ unethical conduct from top management perspective
Authors: Jha Jatinder Kumar , Singh Manjari
Published: July 2021

Purpose

The purpose of the study is to explore the various kind of prevailing unethical practices at workplace along with identification of factors triggering such unethical practices. Growing incidences of indulgence of employees in unethical acts in various organisation and negative consequences associated with it for the organisation such as erosion of reputation because of advance digital media coverage, shareholder value and others made compulsive to study the root cause of unethical behaviour at the workplace.

Design/methodology/approach

This study extracts meaning from the experiences of top managers working in nine Indian organisations to understand the challenges faced by individuals at the workplace using the Gioia methodology. A total of 33 top management team (TMT) members were interviewed in detail to capture their experience in regard to various challenges that impose a threat to ethical conduct in the organisation.

Findings

The authors identified four categories of unethical behaviour, namely, pro-self, lack of autonomy, pro-organisation, systemic and negligence. Further, the authors have developed a taxonomy suggesting strategies to control unethical conduct at the workplace. Besides, the current study unravels the triggers behind different categories of unethical conduct, such as bottom-line mentality, rent-seeking behaviour of government officials, fluid ethical study culture and others.

Originality/value

Various types of unethical behaviour have been identified and frameworks to address such unethical practices are suggested in the paper. TMTs views have been captured to understand the root cause of unethical practices and strategies for addressing them have been discussed in the paper.
Innovations and challenges in SDG integration and reporting in higher education: a case study from the University of South Florida
Authors: Hansen Brooke , Stiling Peter , Uy Whitney Fung
Published: July 2021

Purpose

As the UN Sustainable Development Goals (SDGs) have been widely adopted since 2015, higher education institutions (HEIs) are experimenting with ways they can be measured, reported and incorporated into all realms of the university. In this process, the challenges of SDG integration into HEIs have become more evident, from lack of resources and sustainability literacy to having multiple disconnected programs that feature the SDGs.

Design/methodology/approach

A mixed methods approach using archival materials, literature reviews, interviews and participant observation has been adopted for a case study at a university in the process of adopting the SDGs in multiple areas.

Findings

The University of South Florida began with efforts to incorporate SDGs at the undergraduate level, such as the Global Citizens Project that brands both events and courses with SDGs. Institutional changes coupled with the launch of the Times Higher Education University Impact Rankings provided more opportunities to have broader conversations about SDGs in all areas of campus.
The exclusion of migrants and refugees from welfare programs in Austria: the “legitimizing explanations” across different policy areas
Authors: Landini Irene
Published: July 2021

Purpose

The present article deals with the topic of migrants’ exclusion from welfare benefits in European host countries from the angle of the research on the so-called “welfare chauvinism” (Andersen and Bjørklund 1990, p. 212). More specifically, it explores the political justifications behind welfare chauvinism in the policy debate surrounding some recent chauvinist-oriented social policies. Drawing on that, the article develops a theoretical argument to generate expectations about how politicians use different types of justifications. The fundamental proposition is that the chauvinistic arguments used are shaped by the different types of social programs, i.e. either universal or means-tested programs.

Design/methodology/approach

Qualitative content analysis of several selected parliamentary debates in the period 2017–2019 in Austria is carried out. In order to improve the efficiency of the research, the author relies on MAXQDA, an advanced piece of software for qualitative data analysis, to code the qualitative data and analyze them. The author prefers this to other similar programs as it is considered a valid and reliable tool within the academic research world.

Findings

The article points out that programs design works as an explanatory factor to highlight variations of welfare chauvinist arguments.

Originality/value

It develops for the first time a theoretical argument explaining the presence and variation of welfare chauvinist arguments based on social programs design.
Effect of micro-textures on lubrication characteristics of spur gears under 3D line-contact EHL model
Authors: Zhao Jiang , Li Zhengminqing , Zhang Hong , Zhu Rupeng
Published: July 2021

Purpose

The purpose of this paper is to use a combination of numerical simulation and experiment to evaluate the performance of laser surface texturing (LST) in the field of gear lubrication, and to more accurately predict the lubrication characteristics of different surfaces.

Design/methodology/approach

The method used in this paper is developed on the basis of the deterministic solution of the three-dimensional (3D) mixed elasto-hydrodynamic lubrication (EHL) model and the model parameters are corrected by friction test. The film pressure, film thickness and friction coefficient of different micro-textured tooth surfaces are predicted on the basis of accurate 3D mixed EHL models.

Findings

The results demonstrate that the micro-texture structure of the tooth surface can increase the local film thickness and enhance the lubricating performance of the tooth surface without drastically reducing the contact fatigue life. The stress distribution and friction characteristics of the tooth surface can be optimized by adjusting the micro-texture arrangement and the size of the micro-textures.

Originality/value

A new evaluation method using a 3D hybrid EHL model and friction test to predict the lubrication characteristics of LST is proposed, which can effectively improve the processing economy and save time.

Peer review

The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-11-2020-0423
Hybrid oscillator-based no-delay hip exoskeleton control for free walking assistance
Authors: Yang Wei , Xu Linghui , Yu Linfan , Chen Yuting , Yan Zehao , Yang Canjun
Published: July 2021

Purpose

Walking-aid exoskeletons can assist and protect effectively the group with lower limb muscle strength decline, workers, first responders and military personnel. However, there is almost no united control strategy that can effectively assist daily walking. This paper aims to propose a hybrid oscillators’ (HOs) model to adapt to irregular gait (IG) patterns (frequent alternation between walking and standing or rapid changing of walking speed, etc.) and generate compliant and no-delay assistive torque.

Design/methodology/approach

The proposed algorithm, HOs, combines adaptive oscillators (AOs) with phase oscillator through switching assistive mode depending on whether or not the AOs' predicting error of hip joint degree is exceeded our expectation. HOs can compensate for delay by predicting gait phase when in AOs mode. Several treadmill and free walking experiments are designed to test the adaptability and effectiveness of HOs model under IG.

Findings

The experimental results show that the assistive strategy based on the HOs is effective under IG patterns, and delay is compensated totally under quasiperiodic gait conditions where a smoother human–robot interaction (HRI) force and the reduction of HRI force peak are observed. Delay compensation is found very effective at improving the performance of the assistive exoskeleton.

Originality/value

A novel algorithm is proposed to improve the adaptability of a walking assist hip exoskeleton in daily walking as well as generate compliant, no-delay assistive torque when converging.
The Pransky interview: Dr Nabil Simaan, Vanderbilt University Professor of Mechanical Engineering, Computer Science and Otolaryngology, Thought Leader in Medical Robotics
Authors: Pransky Joanne
Published: July 2021

Purpose

The following article is a “Q&A interview” conducted by Joanne Pransky of Industrial Robot Journal as a method to impart the combined technological, business and personal experience of a prominent, robotic industry PhD and innovator regarding his pioneering efforts. The paper aims to discuss these issues.

Design/methodology/approach

The interviewee is Dr Nabil Simaan, Professor of Mechanical Engineering, Computer Science and Otolaryngology at Vanderbilt University. He is also director of Vanderbilt’s Advanced Robotics and Mechanism Applications Research Laboratory. In this interview, Simaan shares his unique perspective and approaches on his journey of trying to solve real-world problems in the medical robotics area.

Findings

Simaan received his BSc, MSc and PhD in mechanical engineering from the Technion – Israel Institute of Technology. He served as Postdoctoral Research Scientist in Computer Science at Johns Hopkins University. In 2005, he joined Columbia University, New York, NY, as an Assistant Professor of Mechanical Engineering until 2010, when he joined Vanderbilt. His current applied research interests include synthesis of novel robotic systems for surgical assistance in confined spaces with applications to minimally invasive surgery of the throat, natural orifice surgery, cochlear implant surgery and dexterous bimanual microsurgery. Theoretical aspects of his research include robot design and kinematics.

Originality/value

Dr Simaan is a leading pioneer on designing robotic systems and mechanisms for medical applications. Examples include technologies for snake robots licensed to Intuitive Surgical; technologies for micro-surgery of the retina, which led to the formation of AURIS Surgical Robotics; the insertable robotic effector platform (IREP) single-port surgery robot that served as the research prototype behind the Titan Medical Inc. Sport (Single Port Orifice Robotic Technology). Simaan received the NSF Career award for young investigators to design new algorithms and robots for safe interaction with the anatomy. He has served as the Editor for IEEE International Conference on Robotics and Automation, Associate Editor for IEEE Transactions on Robotics, Editorial Board Member of Robotica, Area Chair for Robotics Science and Systems and corresponding Co-chair for the IEEE Technical Committee on Surgical Robotics. In January 2020, he was bestowed the award of Institute of Electrical and Electronics Engineers (IEEE) Fellow for Robotics Advancements. At the end of 2020, he was named a top voice in health-care robotics by technology discovery platform InsightMonk and market intelligence firm BIS Research. Simaan holds 15 patents. A producer of human capital, his education goal is to achieve the best possible outcome with every student he works with.