bluuebunny/crossref_metadata_2025 huggingface dataset:
Primary Prostatic Stromal Sarcoma: A Case Report and Review of the Literature
Authors: Erul Enes , Gülpınar Ömer , Kuru Öz Diğdem , Berber Havva , Kiremitci Saba , Ürün Yüksel
Published: November 2024

Background and Objectives: Primary prostatic stromal sarcoma is an exceptionally rare urological malignancy, constituting less than 0.1% of all prostatic cancers. It poses a significant clinical challenge due to its aggressive behavior and poor prognosis. Materials and Methods: We report the case of a 34-year-old male who presented with nonspecific lower urinary tract symptoms, including dysuria and increased urinary frequency. The initial diagnostic workup, including digital rectal examination and Magnetic Resonance Imaging (MRI), revealed a lobulated lesion within the prostate. A transurethral resection (TUR) was performed for diagnostic purposes, and histopathological examination revealed a “malignant mesenchymal tumor”. The patient underwent a laparoscopic radical prostatectomy. The pathology report confirmed the diagnosis of prostatic stromal sarcoma. The postoperative follow-up, including systemic CT and MRI, showed no evidence of recurrence or metastasis thus far. Results: Multidisciplinary management is essential for optimizing treatment outcomes in all urologic malignancies; however, it becomes particularly challenging and crucial in rare cases such as primary prostatic stromal sarcoma. In our case, the patient benefited from a coordinated approach involving urology, pathology, and oncology, underscoring the importance of collaborative care for rare and aggressive tumors like this. This case highlights the importance of early detection, complete surgical excision, and consideration of adjuvant therapies, given the aggressive nature of the disease. The role of novel therapeutic strategies, including immunotherapy and targeted therapies, is also discussed in the context of metastatic sarcomas. Conclusions: This case underscores the critical need for a comprehensive, multidisciplinary approach to managing primary prostatic stromal sarcoma. Ongoing research on innovative therapies offers hope for improved outcomes in metastatic stages.
Impact of Vaccination Status on COVID-19 Severity and Pulmonary Involvement
Authors: Laitin Sorina Maria Denisa , Baditoiu Luminita Mirela , Laza Ruxandra , Besliu Razvan Sebastian , Stoicescu Emil Robert , Gug Miruna , Dumitru Cristina Stefania , Patrascu Raul
Published: November 2024

Background and Objectives: The COVID-19 pandemic has had a significant impact on global health, with serious outcomes, such as lung damage, being major determinants of patient morbidity and mortality. Immunization has been essential in attenuating these outcomes. This study aimed to evaluate the impact of COVID-19 vaccination on disease severity, particularly focusing on pulmonary involvement, among hospitalized patients. Materials and Methods: A retrospective cohort study was conducted at Victor Babes Hospital, Timisoara, involving 3005 patients diagnosed with COVID-19 between December 2020 and March 2022. Patients were stratified into vaccinated and unvaccinated groups. Results: The study found that vaccinated patients had significantly lower rates of severe pulmonary involvement compared to unvaccinated patients. Specifically, only 24.24% of vaccinated patients experienced severe lung involvement, compared to 35.64% in the unvaccinated group (p < 0.001). Vaccinated individuals had shorter hospital stays (8.96 ± 6.40 days vs. 10.70 ± 6.29 days), but this difference was not statistically significant (p = 0.219). Additionally, chronic pulmonary diseases and stroke were less prevalent among vaccinated patients, highlighting the protective effect of vaccination. Conclusions: COVID-19 vaccination significantly reduces the severity of disease, particularly in preventing severe pulmonary involvement, which is a major determinant of patient outcomes. These findings underscore the importance of ongoing vaccination efforts and the need for booster doses to maintain immunity, especially as new variants emerge. The study supports the continued prioritization of vaccination in public health strategies to mitigate the long-term impact of COVID-19.
The Impact of Telemonitoring and Telehealth Coaching on General Nutrition Knowledge in Overweight and Obese Individuals: A Pilot Randomized Controlled Trial
Authors: Eid Noura M. S. , Al-Ofi Ebtisam A. , Enani Sumia , Mosli Rana H. , Saqr Raneem R. , Qutah Karimah M. , Eid Sara M. S.
Published: November 2024

(1) Background: General nutrition knowledge is a fundamental pillar of well-being and healthy lifestyles. This study aimed to measure the general nutrition knowledge questionnaire (GNKQ) scores of overweight and obese participants who joined a pilot randomized controlled trial (RCT) and the association between changes in GNKQ scores and changes in anthropometric measures. (2) Methods: A total of 30 and 25 participants had completed the trial at the 3- and 6-month visits, respectively. All participants enrolled in a randomized controlled trial (RCT) and received a hypocaloric-tailored diet and three online nutrition education sessions over 6 months. The participants were randomly divided into two groups: an intervention group supported with weekly telemonitoring and monthly telehealth coaching vs. a control group. The Arabic-validated GNKQ was used, covering four sections: dietary recommendations; food groups and nutrient sources; healthy food choices; and associations between the diet–disease relationship and weight. (3) Results: The findings show that both the intervention and control groups showed improvements in GNKQ scores over time, with the intervention group demonstrating significant increases in overall nutrition knowledge and specific areas, such as the diet–disease relationship and weight management, at 3 months. In addition, changes in GNKQ scores had a significant negative association with BMI and visceral fat percentage. The findings underline the benefits of supporting dietary weight loss interventions with telemonitoring and telehealth coaching, suggesting that an increase in nutrition knowledge may relate to lower body fat metrics. Nevertheless, the small sample size and high attrition rate of participants were the main limitations of this study, such that large populations are required to confirm the reliability of the obtained findings.
Anion-Exchange Membranes’ Characteristics and Catalysts for Alkaline Anion-Exchange Membrane Fuel Cells
Authors: Su Fa-Cheng , Yu Hsuan-Hung , Yang Hsiharng
Published: November 2024

This work aims at the effects of anion-exchange membranes (AEMs) and ionomer binders on the catalyst electrodes for anion-exchange membrane fuel cells (AEMFCs). In the experiments, four metal catalysts (nano-grade Pt, PtRu, PdNi and Ag), four AEMs (aQAPS-S8, AT-1, X37-50T and X37-50RT) and two alkaline ionomers (aQAPS-S14 and XB-7) were used. They were verified through several technical parameters examination and cell performance comparison for the optimal selection of AMEs. The bimetallic PdNi nanoparticles (PdNi/C) loaded with Vulcan XC-72R carbon black were used as anode electrodes by using the wet impregnation method, and Ag nanoparticles (Ag/C) were used as the catalyst cathode. It was found that the power density and current density of the X37-50RT are higher than the other three membranes. Also, alkaline ionomers of XB-7 had better performance than aQAPS-S14. The efficiency was improved by 32%, 155% and 27%, respectively, when compared to other membranes by using the same catalyst of PdNi/C, Ag/C and Pt/C. The results are consistent with the membrane ion conductivity measurements, which showed that the conductivity of the X37-50RT membrane is the highest among them. The conductivity values for hydroxide ions (OH−) and bromide ions (Br−) are 131 mS/cm and 91 mS/cm, respectively. These findings suggest that the properties (water uptake, swelling rate and mechanical) of the anion-exchange membrane (AEM) can serve as a key reference for AEM fuel cell applications.
Verification of Numerical Models of Steel Bar Coverings Using Experimental Tests—Preliminary Study
Authors: Zabojszcza Paweł , Radoń-Kobus Krystyna , Kossakowski Paweł Grzegorz
Published: November 2024

In the design of metal bar coverings, the key problem is to correctly determine the numerical model of the analyzed structure. The description of numerical models may differ from the actual, real behavior of the structure. Therefore, there is a need to verify and calibrate them using experimental studies. The aim of this research will be to verify and assess the accuracy of the numerical model of a metal bar roof by conducting experimental studies. A series of repeatable experimental tests will be conducted on the structure model to determine the path of static equilibrium and the form of stability loss of the steel covering. During the test, as the load increases, data will be collected on the displacements of nodes. The displacements of the nodes will be verified using precise triangulation laser sensors and electronic sensors. Based on the results of the tests, conclusions will be drawn regarding the accuracy of the numerical models. Comparison of the results obtained from the numerical models with the experimental data will allow for the identification of possible discrepancies and understanding how the numerical models can be improved. This in turn will contribute to the development of more advanced and more accurate methods for the analysis of metal bar roof structures in the future.
Analysis of the Effect of Fluxing Additives in the Production of Titanium Slags in Laboratory Conditions
Authors: Myrzakulov Maxat K. , Dzhumankulova Saltanat K. , Yelemessov Kassym K. , Barmenshinova Madina B. , Martyushev Nikita V. , Skeeba Vadim Y. , Kondratiev Viktor V. , Karlina Antonina I.
Published: November 2024

The article theoretically justified and experimentally confirmed the possibility of implementing the process of the electric melting of Satbayevskiy ilmenite concentrates with new fluxing additives based on oxides and nitrides of aluminium, calcium, and boron. They also include boron carbonitride CNV that will expand the raw material base of Kazakhstan titanium production by involving local substandard material in the process, as well as the technical and economic performance of electric melting. In order to conduct experiments in the area of extremum, the ilmenite concentrate from the Satbayev deposit was taken. Furthermore, the optimum conditions of the electric melting of Satbayevski ilmenite concentrates (such as the process temperature of 1550–1600 °C, the reducing agent consumption of 8–10% of the concentrate mass, and the duration of 90 min), using new fluxing additives, were selected. As a result of the experiment (performed at a temperature of 1600 °C), it has been found that the introduction of 3 to 6% of fluxes in the charge of electric melting promotes the reduction of iron oxides from 45 to 80% and achievement of the extraction of titanium oxide in slag of up to 83.5–90.1%. The addition of 6% boron oxide and carbonitride in the charge of electric melting reduces the melting temperature of the charge to ~1400–1450 °C and the melting time to 90 min. It also creates conditions for a quieter electric melting mode.
Investigation of Carbothermic Reduction of Ilmenite Concentrate with Calcium Carbonate Addition Using the Rietveld Method
Authors: Wang Xiaoli , Li Yang , Wang Huajun , Sun Chunbao , Kou Jue
Published: November 2024

The carbothermic reduction of ilmenite concentrate was performed with the addition of calcium carbonate (CaCO3) as an additive. The effects of roasting temperature and CaCO3 content on calcium titanate (CaTiO3) formation and metallic iron reduction were investigated. The Rietveld method was employed for qualitative and quantitative analysis of roasting products. It was found that as the temperature increased from 1000 °C to 1400 °C, the calcium titanate content increased from 21.95% to 83.02%. Similarly, as the CaCO3 content increased, the calcium titanate content rose from 0% to 83.11%, while the metallic iron content increased from 4.52% to 10.37%. SEM-EDS analysis suggested that adding CaCO3 during the carbothermic reduction of ilmenite promoted the aggregation and growth of metallic iron particles. The roasting products obtained under optimal conditions were subjected to three-stage grinding and three-stage magnetic separation. The final metallic iron product had a grade of 90.01% and a recovery rate of 91.32%. The calcium titanate product contained 55.43% TiO2, with a titanium recovery rate of 85.90%. Rietveld results indicated that the calcium titanate product had a high purity, with a content of 90.87%. This study effectively improved the comprehensive utilization of titanium and iron resources in ilmenite.
In Situ Pre-Metallization Cleaning of CoSi2 Contact-Hole Patterns with Optimized Etching Process
Authors: Choi Tae-Min , Jung Eun-Su , Yoo Jin-Uk , Lee Hwa-Rim , Yoon Songhun , Pyo Sung-Gyu
Published: November 2024

We examined how controlling variables in a pre-metallization Ar sputter-etching process for in situ contact-hole cleaning affects the contact-hole profile, etching rate, and substrate damage. By adjusting process parameters, we confirmed that increasing plasma power lowered the DC bias but enhanced the etching rate of SiO2, while increasing RF power raised both, with RF power having a more pronounced effect. Higher Ar flow rate reduced etching uniformity and slightly lowered the DC bias. There was no significant difference in the amount of etching between the oxide film types, but the nitride/oxide selectivity ratio was about 1:2. Physical damage during Ar sputter-etching was closely linked to DC bias. finally, Finally, etching of the Si and CoSi2 sublayers was performed on the device contact hole model. At this time, Si losses of up to about 31.7 Å/s occurred, and the etch speed was strongly affected by the DC bias. By optimizing the RF power and plasma power, we achieved a Si/CoSi2 etch selectivity ratio of about 1:2.
Biological Traits of Vertically Seed-Transmitted Bacillus mojavensis in Triticum aestivum L.
Authors: Diabankana Roderic Gilles Claret , Afordoanyi Daniel Mawuena , Filimonova Maria Nikolaevna , Validov Shamil Zavdatovich , Safin Radik Ilyasovich
Published: November 2024

Seed-borne endophytic bacteria can influence host responses to biotic and abiotic stress conditions. Their presence in seeds is related to their ability to colonize plant tissues and to pass from parent plants to offspring. In this study, we investigated the ability of Bacillus mojavensis PS17 to pass into the next generation of spring wheat plants via seeds and the effect of the transmission mode on the functional traits of seed-transmitted colonies of PS17. The rifampicin-resistant PS17 strain at 100 µg/mL was used to track PS17 effectively throughout the wheat growth cycle. The results demonstrated the successful colonization of B. mojavensis PS17 and its ability to pass into the next plant generation through seeds. During plant development, the PS17 cell population was almost higher in the rhizosphere than in the aboveground parts of plants, including seeds at the grain-filling stage. The seed-transmitted B. mojavensis PS17 colonies exhibited identical biological traits to those of the parental PS17 strain. Bacillus mojavensis PS17 retained its ability to suppress the growth of pathogens, such as Fusarium oxysporum and Alternaria alternata, and produce hydrolases, including protease, lipase, amylase, and cellulase. These results highlight the potential of vertical transmission through seeds as a mode of spreading bacterial biocontrol agents in future plants.
Structural and Kinematic Analysis of the Xipu Dome in the Tingri Area, Southern Tibet, and New Exploration Discoveries
Authors: Yan Songtao , Ding Ailing , Wang Jie , Huang Hao , Li Hu , Chen Song , Liu Tao , Zhu Lidong
Published: November 2024

The newly delineated Xipu Dome, located in the central North Himalayan Gneiss Dome (NHGD), exhibits a significant spatiotemporal relationship with Himalayan polymetallic mineralization. Based on field geological surveys and geochronological analyses, this study provides a comprehensive assessment of the lithological assemblage, tectonic deformation, and metallogenic processes of the Xipu Dome. The findings reveal a three-tiered structure: the core consists of early Paleozoic granitic gneiss (523 Ma) and Miocene leucogranite (13.5 Ma), overlain by a cover of low-grade metamorphic or unmetamorphosed sedimentary rocks, and a detachment zone composed of heavily deformed schists and phyllites. The Xipu Dome underwent three phases of tectonic deformation: a southward thrust caused by continental collision, northward extensional activity driven by the South Tibet Detachment System (STDS), and gravitational collapse and downslope sliding following the emplacement of the dome. Two types of mineralization were identified: structural hydrothermal Au-Cu polymetallic deposits related to detachment and skarn-type Cu-Ag polymetallic deposits associated with leucogranite intrusion. This study enhances the understanding of the spatial distribution and metallogenic potential within the Himalayan Be-Sn rare metal-Pb-Zn-Sb-Au belt, offering a valuable direction for strategic mineral exploration in the Tethyan Himalaya (TH).
Sono- and Zeolite-Assisted Electrocoagulation for Compost Wastewater Treatment: Does Ultrasound Power Make a Difference?
Authors: Vukojević Medvidović Nediljka , Vrsalović Ladislav , Svilović Sandra , Gudić Senka , Čule Ivona
Published: November 2024

This study builds on previous research that investigated the triple hybrid wastewater treatment system combining electrocoagulation (EC), zeolite (Z), and ultrasound (US), and it examines the effects of different ultrasonic powers on compost wastewater treatment by analysing several process parameters. In the first part, two levels of US power intensity, corresponding to 20% and 100% of intensity, with and without zeolite addition, were investigated on three different electrode materials. Although satisfactory chemical oxygen demand (COD) reduction was obtained (81.42%–88.90%), better results were obtained for Al and Zn electrodes at 20% US power intensity, while for Fe better resulted were obtained at 100% US power intensity. Deteriorations of the anodic and cathodic surfaces, which were analysed using optical microscope images at 50× and 200× magnification, are generally less pronounced at higher US power intensities. Energy consumption in the range of 3.86–18.78 kWh/m3 showed an increased sequence—Fe < Zn < Al. In the second part, Taguchi optimisation was used to study the influence of US intensity (0%, 20%, 100%), the electrode materials (Al, Fe, or Zn), contact time (10, 20, and 30 min), and mixing speed (150, 250, 350 rpm) on COD decrease, electrode consumption, settling velocity, and voltage (power) consumption. The result shows that increased US power enhances COD reduction, electrode mass preservation, and voltage while decreasing the settling velocity.
Silver Nanoparticles as a Potent Nanopesticide: Toxic Effects and Action Mechanisms on Pest Insects of Agricultural Importance—A Review
Authors: Martínez-Cisterna Daniel , Rubilar Olga , Tortella Gonzalo , Chen Lingyun , Chacón-Fuentes Manuel , Lizama Marcelo , Parra Pablo , Bardehle Leonardo
Published: November 2024

Nanotechnology has been a promising plant protection discipline in recent years, attributed to the unique physicochemical properties exhibited at the nanoscale. In this context, silver nanoparticles (AgNPs) have been effective in various applications, including medical, industrial, and agronomic, and during the last few years, the control of insect pests has raised great interest. The present review mainly provides updated information about the use of AgNPs elaborated by different synthesis methods, such as biological (plants, microorganisms), physical, and chemical, and their effect against various insect species of agricultural importance belonging to the order Diptera, Coleoptera, Lepidoptera, and Hemiptera. The physiological and toxic effects of applying AgNPs are reported and characterized by developmental problems, mortality, weight reduction, interference with enzymatic activity, and anomalies in the life cycle. Moreover, in the final section, the action mechanisms through which AgNPs act on insects are also discussed, highlighting mechanisms such as alteration of transmembrane permeability, interruption of DNA replication, alteration of protein synthesis, and production of reactive oxygen species (ROS).
Effects of Ethylene Propylene Diene Monomer (EPDM)-Based Polar Macromolecular Compatibilizers on the Low-Temperature Properties of Fluoroelastomer/EPDM Rubber Blends
Authors: Liu Gen , Du Faxin , Yao Zhangjun , Li Guangzhao , Kuang Wen , Zhu Chongyu , Liu Yi , Chen Honglin , Wang Fumei , Zhou Ce , Wei Xueli , Wang Wenyan , Han Rui
Published: November 2024

Integrating rubber with superior low-temperature capabilities, such as ethylene propylene diene monomer (EPDM), is a strategic approach to bolster the low-temperature performance of fluoroelastomer (FKM). However, FKM and EPDM are thermodynamically incompatible. This work synthetized three EPDM-based polar macromolecular compatibilizers, epoxidized EPDM (EPDM-EP), 2,2-trifluoroethylamine-grafted epoxidized EPDM (EPDM-TF), and 2,4-difluorobenzylamine-grafted epoxidized EPDM (EPDM-DF), to enhance the compatibility between FKM and EPDM. These compatibilizers were subsequently incorporated into FKM/EPDM rubber blends. The results revealed that the glass transition temperature (Tg) of FKM/EPDM decreased by 1.3 °C, 2.68 °C, and 2.78 °C, respectively, upon the addition of 10 phr of EPDM-EP, EPDM-TF, or EPDM-DF. Moreover, the Tg of the two phases converged. The tensile strength, elongation at break, and tear strength of the FKM/EPDM rubber blends were also enhanced by the inclusion of these compatibilizers. Notably, EPDM-TF and EPDM-DF exhibited remarkable compatibilization effects due to an increase in polarity. This research not only sheds light on the potential for developing new compatibilizers but also paves the way for innovative applications of FKM and its derivatives.
Diversity of Microglia-Derived Molecules with Neurotrophic Properties That Support Neurons in the Central Nervous System and Other Tissues
Authors: Wiens Kennedy R. , Wasti Naved , Ulloa Omar Orlando , Klegeris Andis
Published: November 2024

Microglia, the brain immune cells, support neurons by producing several established neurotrophic molecules including glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF). Modern analytical techniques have identified numerous phenotypic states of microglia, each associated with the secretion of a diverse set of substances, which likely include not only canonical neurotrophic factors but also other less-studied molecules that can interact with neurons and provide trophic support. In this review, we consider the following eight such candidate cytokines: oncostatin M (OSM), leukemia inhibitory factor (LIF), activin A, colony-stimulating factor (CSF)-1, interleukin (IL)-34, growth/differentiation factor (GDF)-15, fibroblast growth factor (FGF)-2, and insulin-like growth factor (IGF)-2. The available literature provides sufficient evidence demonstrating murine cells produce these cytokines and that they exhibit neurotrophic activity in at least one neuronal model. Several distinct types of neurotrophic activity are identified that only partially overlap among the cytokines considered, reflecting either their distinct intrinsic properties or lack of comprehensive studies covering the full spectrum of neurotrophic effects. The scarcity of human-specific studies is another significant knowledge gap revealed by this review. Further studies on these potential microglia-derived neurotrophic factors are warranted since they may be used as targeted treatments for diverse neurological disorders.
Thermooxidation of Four Sartans: Kinetic Analysis Based on Thermo-Gravimetric Data
Authors: Ledeţi Adriana , Baul Bianca , Ridichie Amalia , Ivan Denisa , Vlase Titus , Tomoroga Carmen , Dragomirescu Anca , Vlase Gabriela , Bertici Răzvan Adrian , Man Dana Emilia , Ledeţi Ionuţ
Published: November 2024

Angiotensin II receptor antagonists are tetrazole derivatives used in the treatment of high blood pressure, and are also indicated for the treatment of heart failure (NYHA class II-IV). They are used alone or in combination with other classes of antihypertensives or diuretics for the effective management of high blood pressure. In this study, we aim to evaluate the thermal stability and degradation kinetics for the principal compounds used in therapy from this class, namely telmisartan, valsartan, olmesartan medoxomil, and losartan potassium. To obtain the thermoanalytical data for the kinetic investigations, the TG and DTG curves were registered at five different heating rates (β = 2, 4, 6, 8, and 10 °C min−1). The kinetic methods used were a preliminary ASTM E698 method and two isoconversional methods: Flynn–Wall–Ozawa and Friedman. For each molecule, the results showed complex decomposition processes consisting of complex reaction sequences.
Oblique Deposited Ultra-Thin Silver Films on Polymer Gratings for Sensitive SERS Performance
Authors: Jen Yi-Jun , Lin Meng-Jie
Published: November 2024

A small amount of silver was obliquely deposited onto a polymer subwavelength grating to form a metasurface that comprised silver split-tubes. An ultra-thin silver film with a monitor-controlled thickness of 20 nm at the corner of each ridge of the grating provided the most sensitive surface-enhanced Raman scattering (SERS) measurements. An excitation laser beam that was incident from the substrate provided similar or better SERS enhancement than did the general configuration with the laser beam incident directly on the surface of the nanostructure. Near-field simulations were conducted to model the localized electric field enhancement and to quantify the SERS performance, demonstrating the effectiveness of this novel deposition method.
Syngas from Reforming Methane and Carbon Dioxide on Ni@M(SiO2 and CeO2)
Authors: Hua Derun , Li Jian , Guo Xiaowen , Lu Xinning , Ding Hao , He Rengui
Published: November 2024

The accumulation of greenhouse gasses (CH4 and CO2) results in an increase in the temperature of the atmosphere. The conversion of greenhouse gasses into chemicals and fuels with high added value benefits not only the environment but also energy development. A promising and well-studied process is the reforming of methane, where CH4 and CO2 are converted into syngas (CO and H2). However, catalysts hinder the development of the process. In this paper, we investigate the conversion of CH4 and CO2 into syngas using a thermal conversion method. The catalysis performance was evaluated by reforming methane. Ni-based catalysts were prepared by different methods. All prepared catalysts were characterized (XRD, HRTEM et al.), and the process of reforming carbon dioxide–methane was carried out in a fixed bed reactor under atmospheric pressure and a high temperature. Ni(M) @CeO2 is one of the most popular options due to the role of CeO2. The deposition of coke in Ni-based catalysts was investigated.
Impact of Kiwifruit Consumption on Cholesterol Metabolism in Rat Liver: A Gene Expression Analysis in Induced Hypercholesterolemia
Authors: Gorji Abdolvahab Ebrahimpour , Ciecierska Anna , Leontowicz Hanna , Roudbari Zahra , Sadkowski Tomasz
Published: November 2024

Background/Objectives: Cholesterol is vital in various bodily functions, such as maintaining cell membranes, producing hormones, etc. However, imbalances, like hypercholesterolemia, can lead to diseases such as cancer, kidney disease, non-alcoholic fatty liver disease, and cardiovascular conditions. This study explores the impact of kiwifruit consumption, specifically Actinidia arguta cultivar Geneva and Actinidia deliciosa cultivar Hayward, on cholesterol and lipid metabolism in rat liver. Methods: Rats were divided into groups: a 1% cholesterol control group (Ch), a 5% Geneva kiwifruit-supplemented group (ChGENE), and a 5% Hayward kiwifruit-supplemented group (ChHAYW). Gene expression was analyzed using Gene Spring v.14. Gene ontology, pathway analysis, miRNA, and transcription factor prediction were performed using DAVID, Reactome, and miRNet. In addition, we used Agilent Literature Search software to gain further insights. Results: Statistical analysis identified 72 genes in ChGENE-Ch and 2 genes in ChHAYW-Ch comparison. Key genes involved in cholesterol metabolism pathways, including PCSK9, SCD1, SLC27A5, HMGCR, and DHCR24, showed lower expression in the kiwifruit-supplemented groups. The genes mentioned above showed lower expression in the kiwifruit-supplemented group, probably contributing to the liver lipid level reduction. Further analysis identified miRNA-26a, miRNA-29a/b/c, miRNA-33a/b, and miRNA-155 targeting hub genes. Conclusions: Our findings suggest that dietary supplementation with kiwifruit, particularly the Geneva cultivar, reduces fat accumulation in the liver of rats with hypercholesterolemia, likely through downregulation of critical genes involved in cholesterol metabolism. These studies highlight the potential of kiwifruit as a part of a dietary strategy to manage cholesterol levels.
Relationship Between Dietary Nutrient Intake and Autophagy—Related Genes in Obese Humans: A Narrative Review
Authors: Bednarczyk Martyna , Dąbrowska-Szeja Nicola , Łętowski Dariusz , Dzięgielewska-Gęsiak Sylwia , Waniczek Dariusz , Muc-Wierzgoń Małgorzata
Published: November 2024

Obesity is one of the world’s major public health challenges. Its pathogenesis and comorbid metabolic disorders share common mechanisms, such as mitochondrial or endoplasmic reticulum dysfunction or oxidative stress, gut dysbiosis, chronic inflammation and altered autophagy. Numerous pro-autophagy dietary interventions are being investigated for their potential obesity-preventing or therapeutic effects. We summarize current data on the relationship between autophagy and obesity, and discuss various dietary interventions as regulators of autophagy-related genes in the prevention and ultimate treatment of obesity in humans, as available in scientific databases and published through July 2024. Lifestyle modifications (such as calorie restriction, intermittent fasting, physical exercise), including following a diet rich in flavonoids, antioxidants, specific fatty acids, specific amino acids and others, have shown a beneficial role in the induction of this process. The activation of autophagy through various nutritional interventions tends to elicit a consistent response, characterized by the induction of certain kinases (including AMPK, IKK, JNK1, TAK1, ULK1, and VPS34) or the suppression of others (like mTORC1), the deacetylation of proteins, and the alleviation of inhibitory interactions between BECN1 and members of the Bcl-2 family. Significant health/translational properties of many nutrients (nutraceuticals) can affect chronic disease risk through various mechanisms that include the activation or inhibition of autophagy. The role of nutritional intervention in the regulation of autophagy in obesity and its comorbidities is not yet clear, especially in obese individuals.
Visualizing Plant Disease Distribution and Evaluating Model Performance for Deep Learning Classification with YOLOv8
Authors: Ghafar Abdul , Chen Caikou , Atif Ali Shah Syed , Ur Rehman Zia , Rahman Gul
Published: November 2024

This paper presents a novel methodology for plant disease detection using YOLOv8 (You Only Look Once version 8), a state-of-the-art object detection model designed for real-time image classification and recognition tasks. The proposed approach involves training a custom YOLOv8 model to detect and classify various plant conditions accurately. The model was evaluated using a testing subset to measure its performance in detecting different plant diseases. To ensure the model’s robustness and generalizability beyond the training dataset, it was further tested on a set of unseen images sourced from Google Images. This additional testing aimed to assess the model’s effectiveness in real-world scenarios, where it might encounter new data. The evaluation results were auspicious, demonstrating the model’s capability to classify plant conditions, such as diseases, with high accuracy. Moreover, the use of YOLOv8 offers significant improvements in speed and precision, making it suitable for real-time plant disease monitoring applications. The findings highlight the potential of this methodology for broader agricultural applications, including early disease detection and prevention.
Clinical Study of Vitamin D Levels in Hospitalized Children with Acute Respiratory Infections
Authors: Petkova Gena Stoykova , Mineva Eleonora Nikolaeva , Botsova Venetsia Tsvetkova
Published: November 2024

The aim of our research was to evaluate and analyze serum 25(OH) vitamin D and parathyroid hormone (PTH) levels to investigate whether vitamin D deficiency serves as a risk factor for an increased incidence of acute respiratory infections (ARI) in children. Serum PTH levels were used as an indicator of vitamin D sufficiency, as normal PTH levels require an optimal concentration of 25(OH) vitamin D. The study included 129 children, divided into five subgroups: children with acute bronchopneumonia (n = 42), acute laryngotracheitis (n = 7), acute bronchiolitis (n = 32), acute bronchitis (n = 18), and a control group (n = 30). No statistically significant differences in 25(OH)D levels were observed between the overall population of children with ARI and the control group (p = 0.073). However, significant differences in 25(OH)D levels were identified between the control group and children with bronchopneumonia, acute bronchitis, and laryngotracheitis (p < 0.01, p < 0.05). Regarding PTH levels, statistical significance was found between the control group and the acute bronchiolitis group, due to the high percentage of children with hypervitaminosis in this subgroup. These results highlight the crucial role of vitamin D in the onset and progression of acute respiratory tract infections in children, emphasizing its impact on their overall respiratory health.
Comparison of Drug Delivery Systems with Different Types of Nanoparticles in Terms of Cellular Uptake and Responses in Human Endothelial Cells, Pericytes, and Astrocytes
Authors: Sahin Hakan , Yucel Oguz , Holloway Paul , Yildirim Eren , Emik Serkan , Gurdag Gulten , Tanriverdi Gamze , Erkanli Senturk Gozde
Published: November 2024

Background/Objectives: The key components of the blood–brain barrier (BBB) are endothelial cells, pericytes, astrocytes, and the capillary basement membrane. The BBB serves as the main barrier for drug delivery to the brain and is the most restrictive endothelial barrier in the body. Nearly all large therapeutic molecules and over 90% of small-molecule drugs cannot cross the BBB. To overcome this challenge, nanotechnology, particularly drug delivery systems such as nanoparticles (NPs), have gained significant attention. Methods: Poly(lactide-co-glycolide) (PLGA) and albumin-based NPs (bovine/human), with or without transferrin (Tf) ligands (BSA, HSA, BSA-Tf, HSA-Tf), and nanolipid carriers (NLC) were synthesized. The interactions of these NPs with human brain microvascular endothelial cells (hBMECs), human brain vascular pericytes (hBVPs), and human astrocytes (hASTROs) were analyzed. Results: At doses of 15.62 µg/mL, 31.25 µg/mL, and 62.5 µg/mL, none of the NPs caused toxic effects on hBMECs, hBVPs, or hASTROs after 3 h of incubation. All NPs were internalized by the cells, but BSA-Tf and HSA-Tf showed significantly higher uptake in hBMECs in a dose-dependent manner. Ultrastructural analysis revealed notable differences between NP formulation and cell type. Conclusions: Our findings underscore the potential of ligand-targeted NPs to selectively interact with BBB endothelial cells. Ultrastructural analysis reveals distinct cellular processing pathways for various NP formulations across BBB-associated cell types, with autophagy emerging as a crucial mechanism for NP handling in pericytes and astrocytes. Changes in NP chemical properties upon biological exposure present significant challenges for nanomedicine design, emphasizing the need for further investigation into NP interactions at the cellular and subcellular levels.
Development and Characterization of Spray-Dried Combined Levofloxacin–Ambroxol Dry Powder Inhaler Formulation
Authors: Suraweera Ruwani K. , Spann Kirsten M. , Izake Emad L. , Wells Timothy J. , Wang Xiaodong , Islam Nazrul
Published: November 2024

Background: This study explores the development and characterization of spray-dried composite microparticles consisting of levofloxacin (LVX, a broad-spectrum antibiotic), and ambroxol (AMB, a mucolytic agent that has antibacterial and antibiofilm properties), for the intended application of the drug against lower respiratory tract infections (LRTIs). Methods: A range of LVX to AMB mass ratios (1:1, 1:0.5, and 1:0.25) were prepared, with and without the use of the dispersibility enhancer leucine (LEU), and spray-dried following pre-optimized parameters to achieve the required particle size (1–5 µm) and flow properties. The formulations were characterized by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), and a thermogravimetric analysis (TGA). The in vitro aerosolization performance of the new formulation was evaluated with a twin-stage impinger (TSI) at a flow rate of 60 ± 5 L/min. Using a validated RP-HPLC method, LVX and AMB were quantitatively determined. Results: The combined spray-dried LVX, AMB, and LEU particles were spherically shaped with sizes ranging from 1.9 to 2.9 µm, thus complying with the size requirements for effective deep lung deposition. The dispersibility enhancer leucine produced a high yield and enhanced the flow properties and aerosolization characteristics of the spray-dried formulations. The LVX to AMB mass ratios showed a remarkable impact on the aerosolization properties, with the LVX to AMB 1:1 mass ratio demonstrating the best flow and FPFs for both drugs. There must be a balanced ratio of these components for spray drying the composite particles to obtain composite particles of the required size and with the appropriate flow property. The addition of 5% of LEU significantly (p < 0.005) improved the FPF of all the formulations, probably by enhancing the surface hydrophobicity of the composite particles. Conclusions: The spray-dried combined antibiotics formulation has a strong potential for efficient lung delivery intended for the management of LRTIs.
Attitudes and Experiences of Patients Regarding Gender-Specific Aspects of Pain Management
Authors: Boldt Carolin Alexandra , Keiner Dirk , Best Norman , Bertsche Thilo
Published: November 2024

Background: Biological, pharmacological, and socio-cultural aspects influence gender-specific effects in pain management. Methods: Gender-specific aspects of pain management were assessed in a rural outpatient center via semi-structured patient interview: (i) general gender aspects (total population) from 1 = “fully disagree” to 5 = “fully agree”; and (ii) individual pain (matched pairs) via numeric analog scale (NAS) from 0 = “no pain” to 10 = “maximum pain”. Patient charts were assessed for pain management (WHO-ladder). Results: In total, 113 patients were enrolled (59.18 [SD: 12.76] years, 46% female, 54% male, 0% diverse), and 42 were matched into female-male pairs. (i) Women and men agreed that men and women should be treated equally despite biological differences (median: 5 [women] vs. 5 [men]; p = 0.789). As a reason for gender-specific aspects, “medication concentration” was reported more frequently by women (p = 0.038) and “no answer” by men (p = 0.014). (ii) Mean value (SD) of pain (NAS) was 4.0 (SD 2.3) for women and 3.3 (SD 2.6) for men (p = 0.215) with a positive correlation between pain management escalation (WHO-ladder) and the pain score (NAS) only in men (r = 0.704, p = 0.001). Women rather reported an influence of adverse drug reactions on treatment contentment than men (p = 0.042). Conclusions: Although patients pleaded for gender-independent equal treatment, gender-specific differences in pain therapy were found.
MnOx and Pd Surface Functionalization of TiO2 Thin Films via Photodeposition UV Dose Control
Authors: Stefanov Bozhidar I. , Kolev Hristo G.
Published: November 2024

This study investigated the influence of the ultraviolet (UV) dose (DUV) on the photodeposition of MnOx and Pd cocatalysts on 300-nm-thick anatase TiO2 thin films, which were prepared via sol–gel dip-coating on a glass substrate. MnOx and Pd were photodeposited using increasing UV doses ranging from 5 to 20 J cm−2, from 5 mM aqueous electrolytes based on Mn2+/IO3− or Pd2+, respectively. The effect of the DUV on the MnOx photodeposition resulted in an increase in Mn2+ surface content, from 2.7 to 5.2 at.%, as determined using X-ray photoelectron spectroscopy (XPS). For Pd, increasing the UV dose led to a reduction in the oxidation state, transitioning from Pd2+ to Pd0, while the overall Pd surface content range remained relatively steady at 2.2–2.4 at.%. Both MnOx/TiO2 and Pd/TiO2 exhibited proportional enhancements in photocatalytic activity towards the degradation of methylene blue. Notably, Pd/TiO2 demonstrated a significant improvement in photocatalytic performance, surpassing that of pristine TiO2. In contrast, TiO2 samples functionalized through wet impregnation and thermal treatment in the same electrolytes showed overall lower photocatalytic activity compared to those functionalized via photodeposition.
He-Kr Gas-Discharge Laser Based on Hollow-Core Fiber
Authors: Bufetov Igor , Komissarov Dmitry , Nefedov Sergey , Kosolapov Alexey , Velmiskin Vladimir , Mineev Alexander , Gladyshev Alexey
Published: November 2024

Recently, two completely different types of lasers—a fiber laser and a gas-discharge laser—were combined into a single device by demonstrating 2.03 µm laser generation in He-Xe plasma that was produced by a microwave discharge directly inside a hollow-core fiber. This new type of laser—a gas-discharge fiber laser—provides excellent opportunities to greatly enrich the wavelength range of the operation of fiber lasers. In this work, we investigate a He-Kr gas mixture as an active medium of this new type of laser. As a result, a He-Kr gas-discharge fiber laser is demonstrated for the first time. The laser was pumped by a microwave discharge in a He:Kr (40:1) mixture that was filled into a revolver fiber with the hollow-core diameter of 130 µm. The total gas pressure was about 100 torr. With broadband mirrors of the laser resonator, generation was observed simultaneously at wavelengths of 2190 and 2523 nm. The output power of the He-Kr gas-discharge fiber laser was about 1 mW.
Development of a Tandem Mass Spectral Library for the Detection of Triterpenoids in Plant Metabolome Based on Reference Standards
Authors: Zareena Bibi , Jeelani Syed Usama Y. , Khadim Adeeba , Ali Arslan , Uddin Jalal , Sarker Satyajit D. , Rainer Matthias , Khalifa Shaden A. M. , El-Seedi Hesham R. , Ramzan Muhammad , Musharraf Syed Ghulam
Published: November 2024

Plant triterpenoids represent a diverse group of secondary metabolites and are thought to be valuable for therapeutic applications. For drug development, lead optimization, better knowledge of biological pathways, and high-throughput detection of secondary metabolites in plant extracts are crucial. This paper describes a qualitative method for the rapid and accurate identification of various triterpenoids in plant extracts using the LC-HR-ESI-MS/MS tool in combination with the data-dependent acquisition (DD) approach. A total of 44 isolated, purified, and characterized triterpenoids were analyzed. HR-MS spectra and tandem mass spectra (MS/MS) of each compound were recorded in the positive ionization mode in two different sets of collisional energies, i.e., (25–62.5 eV), and fixed collisional energies (10, 20, 30, and 40 eV). As a result, three triterpenoids were identified in all plant extracts using the retention time, high-resolution mass spectra, and/or MS/MS spectra. The developed method will be helpful with other plant extracts/botanicals, as well as in the search for new triterpenoids in the kingdom Plantae.
Characteristics of Callus and Cell Suspension Cultures of Highbush Blueberry (Vaccinium corymbosum L.) Cultivated in the Presence of Different Concentrations of 2,4-D and BAP in a Nutrient Medium
Authors: Rybin Dmitry A. , Sukhova Alina A. , Syomin Andrey A. , Zdobnova Tatiana A. , Berezina Ekaterina V. , Brilkina Anna A.
Published: November 2024

In this work, cultures of callus and suspension cells originating from leaves of sterile highbush blueberry (Vaccinium corymbosum L.) plants were obtained and characterized. For their active growth and production of phenolic compounds, a combination of 2,4-D at a concentration of 0.34–2.25 µM and BAP at a concentration of 0.45–2.25 µM is effective. An increase in the phytohormone concentration leads to a slowdown in culture formation and reduces their ability to synthesize phenolic compounds. When cultivating V. corymbosum suspension cells over a year (12 passages), they not only retain the ability to synthesize phenolic compounds but also enhance it. By the 12th passage, the content of TSPC in suspension cells reaches 150 mg/g DW, the content of flavonoids reaches 100 mg/g DW, the content of flavans reaches 40 mg/g DW, and the content of proanthocyanidins reaches 30 mg/g DW. The high content of phenolic compounds may be due to the high expression of genes in flavonoid biosynthesis enzymes. V. corymbosum suspension cells accumulate a high level of phenolic compounds during a passage. The ability of V. corymbosum callus and cell suspension cultures in the presence of low concentrations of phytohormones to grow and accumulate biologically active phenolic compounds determines their high economic significance and prospects for organizing a biotechnological method for obtaining phenolic compounds.
The Specific Impacts of Allelopathy and Resource Competition from Artemisia frigida on the Growth of Three Plant Species in Northern China
Authors: Wang Qing , Kong Mengqiao , Wang Junwen , Gao Bin , Ping Xiaoyan
Published: November 2024

Plant interference is a key factor influencing plant coexistence and species composition. The two primary forms of plant interference—allelopathy and resource competition—are often difficult to separate. This study conducted an outdoor pot experiment to quantify the distinct contributions of resource competition and allelopathy of Artemisia frigida on seedling growth of three species: Leymus chinensis, Cleistogenes squarrosa, and Potentilla acaulis. The index of relative neighbor effect (RNE) was used to quantify the overall effect of plant interference, while the inhibition rates (IRs) of resource competition and allelopathy were utilized to determine the specific contributions of allelopathy and resource competition from A. frigida on the growth of target plant species. The interference effect of A. frigida was found to be species-specific. The allelopathic effect of A. frigida played a major role in inhibiting the belowground biomass of L. chinensis (23.97%) and C. squarrosa (58.27%), while allelopathy and resource competition from A. frigida promoted the belowground biomass (45.12%) and aboveground biomass (46.63%) of P. acaulis, respectively. The combined effect of allelopathy and resource competition from A. frigida significantly affected the aboveground biomass of C. squarrosa and P. acaulis, as well as the belowground biomass of L. chinensis and C. squarrosa. These findings contribute to a better understanding of the patterns and mechanisms of plant species composition and its relationship with grazing intensity in this grassland ecosystem.