bluuebunny/crossref_metadata_2025 huggingface dataset:
SERVICE QUALITY AND PRICE ON CUSTOMER SATISFACTION WITH ELECTRONIC WORD OF MOUTH AS MODERATING VARIABLES
Authors: Sadiyah Khalimatus , Lailiyah Elliv Hidayatul , Yaumi Sri , Sawabi Sawabi
Published: January 2023

This study intend to examine the influence of service quality and price on Customer satisfaction with electronic word of mouth as a moderating variable. A quantitative research with a descriptive approach used inthis study. The sample in this study are Customers who make purchases at CV. Amelia Sukses Mandiri Lamongan, totaling 100 respondents. Primary data collected by a questionnaire survey are the data used in this study. Data analysis used Moderated Regression Analysis (MRA). The results showed that service quality had an effect on customer satisfaction. Price has an effect on Customer satisfaction. Electronic word of mouth is able to moderate the effect of service quality on Customer satisfaction, as well as electronic word of mouth is able to moderate the effect of price on Customer satisfaction
PENGARUH MOTIVASI KERJA, LINGKUNGAN KERJA DAN DISIPLIN KERJA TERHADAP PRODUKTIVITAS KERJA KARYAWAN RESTORAN BEBEK HI. SLAMET DI BANDAR LAMPUNG
Authors: Sanida Nuris
Published: January 2023

Restoran Bebek Goreng Pak Slamet telah ada sejak tahun 1986, warung pertama kali Pak Slamet terletak di sepanjang jalan Solo-Yogya. Hasil pengamatan awal ditemukan masalah produktivitas karyawan di restoran Bebek Hi. Slamet Bandar Lampung adalah penurunan produktivitas karyawan dan pendapatan restoran. Penelitian ini yaitu untuk mengetahui produktivitas kerja karyawan Restoran Bebek Hi. Slamet Bandar Lampung dari faktor lingkungan kerja, disiplin kerja, dan motivasi kerja. Metode analisis menggunakan analisis regresi linear berganda dan metode penlitian menggunakan menggunakan kuantitatif. Berdasarkan hasil dan pembahasan dapat disimpulkan bahwa yang berpengaruh positif yaitu lingkungan kerja terhadap produktivitas kerja karyawan, motivasi kerja terhadap produktivitas kerja karyawan dan disiplin kerja terhadap produktivitas kerja karyawan pada Restoran Bebek Hi. Slamet Bandar Lampung. Secara bersama-sama berpengaruh lingkungan kerja, motivasi kerja dan disiplin kerja terhadap produktivitas kerja di Restoran Bebek Hi. Slamet Bandar Lampung.
Prevalence of Syphilis in Pregnancy using a Rapid Treponemal Specific Test in a Low Resource Setting
Authors: Oluwabusayo Oyinloye Olabimpe , Adebayo Adeniyi Augustine , Sunday Awoyinka Babatunde , Timothy Oyekale Oluwalana , Emmanuel Adewara Olumide , Oluseyi Adebara Idowu , Adewumi Bakare , Olabisi Adeyemo Timothy , Olumide Ayankunle Mojisola
Published: January 2023

Background: The rapid treponemal specific test such as immunochromatographic (ICS) test is being recommended as a point of care (POC) test as alternative to the non-specific Treponemal test such as Venereal Disease Research Laboratory (VDRL) commonly used in most developing countries. Objectives: To determine the yields of the ICS test for syphilis among pregnant women. Method: This was a crosssectional study. One hundred and sixty consecutive pregnant women who satisfied the inclusion criteria were recruited into the study. Blood samples obtained from respondents were tested for the presence of Treponema pallidum antibodies using both, Diaspot®syphilis ultra-rapid test kit and MAST-TPHATM TPHA (Treponema pallidum Haemaglutination Assay) test. Results: Out of the 160 pregnant women studied, 4 (2.5%) were sero-reactive for syphilis using the Treponema pallidum haemmaglutination assay (TPHA) while only 2 (1.25%) were sero-reactive to syphilis using the (immunochromatographic screening) ICS method of screening. The sensitivity of the ICS method in detecting syphilis infection was 50% (95% CI: 6.76% – 93.24%) whereas it was 100% specific for syphilis (95% CI: 97.66% -100%). The Positive Predictive Value of the ICS for syphilis screening was 100% while its Negative Predictive value was 98.73% (95% CI: 96.70% – 99.52%). There was no statistically significant difference in the yield of a positive syphilis serology test result comparing the rapid immunochromatographic screening test with the TPHA (P= 0.479). Conclusion: The rapid immunochromatographic screening test (ICS) for syphilis is highly specific and compares favorably with TPHA test in excluding syphilis infection but has a lower sensitivity.
Medical Staff Facing COVID-19 Disease at the University Clinics of Lubumbashi in DR Congo in 2021
Authors: Hendrick Mbutshu Lukuke , Nene Kape Libazi , Abdias Tshibanda kabwebwe , Albert Mwembo Tambwe-ANkoy , Francoise Malonga Kaj , Gédéon Kadima Mukanda
Published: January 2023

Introduction: This study aimed to determine the prevalence of COVID-19 among healthcare workers; to describe the means of prevention used by these nursing staff and to determine the behavior and attitude towards the disease of these nursing staff of the University Clinics of Lubumbashi. Methods: This is a descriptive cross-sectional study from January 1, 2021 to June 31, 2021 among all healthcare providers (doctors and nurses) working at the University Clinics of Lubumbashi. Results: Out of 391 caregivers surveyed, including 246 doctors and 145 nurses, we observed a prevalence of 10.99% that is 43 caregivers, including 28 doctors, ie 65.12% and 15 Nurses or 34.88% whose average age was 40 ±6 years, with a predominance of men (56%) that is sex ratio of 1.26 in favor of men. It is important to note that most cases were diagnosed clinically, ie 62.79% of cases. The saliva droplets were themode of infection of COVID-19 the most experienced by caregivers with 58.14%, followed by physical contact with a rate of 39.53%. The wearing ofmask and hydro-alcoholic friction were the most cited by caregivers as the most effective means of prevention, which they applied and advised patients. Most of the caregivers were subjected to chloroquine and azythromycin, the others admitted to using the traditional treatment of inhaling the vapors of several wild leaves and trees, in particular: lemon, mango... is about the outcome of the disease, almost -all of the nursing staff were cured, ie 97.67% of cases. Conclusion : The prevalence of COVID-19 disease among caregivers was 10.99%, however it is observed that active screening was not carried out and the cases observed presented almost all of the clinical signs and the clinical diagnosis was used for all cases, hence its underestimation. It would be important to regularly screen exposed caregivers and make personal protective equipment regularly available and monitor barrier measures.
An Exploratory Evaluation of a Low Dose Theanine Consumption on Improving Sleep in Middle-Aged and Older Males
Authors: Tominaga Takeichiro , Furushima Daisuke , Unno Keiko , Sugiyama Ibuki , Ozeki Makoto , Nakamura Yoriyuki , Yamada Hiroshi
Published: January 2023

L-theanine (theanine), one of the ingredients of green tea, had been proven to have anti-anxiety, anti-stress, memory, cognitive, and sleep improvement effects in most clinical trials. However, these effects have shown efficacy at a dose of 200–400 mg of theanine a day. In this study, we explored the sleep-improving effects of a lower dose (100 mg/day) of theanine. A double-blind, randomized crossover study was conducted on 25 middle-aged and older males. Participants were randomly assigned to Groups A and B. Group A took theanine first, and Group B took theanine later in the intervention period. The participants took theanine/placebo for one week. Electroencephalogram (EEG) parameters were measured with a monitoring device during sleep, and subjective sleep quality after waking up was evaluated using a questionnaire. Following the statistical analysis between interventions, subgroup analyses according to the participants’ background information were conducted on the representative sleep parameters of non-REM sleep, REM sleep, and wakefulness. Of the 25 participants, three withdrew from this study. In the sleep analysis, there was no significant difference in all sleep parameters between the theanine and placebo periods. In particular, the difference in the percentage of nonREM sleep in stage 2 per sleep duration was not significant (p = 0.053). However, subgroup analysis of the results showed that the percentage of non-REM sleep in stage 2 in groups aged <50 years was higher in theanine than that of the placebo, and in groups with green tea consumption habit of <3 to 4 days per week (p = 0.018, 0.048). The intake of 100 mg/day of theanine does not have an effect on sleep improvement. However, subgroup analyses showed that theanine had an improved effect in stage 2 non-REM sleep according to age and green tea consumption habit.
Influence of Bleeding on Color, Texture, and Chemical Quality of Frozen Pirarucu from Sustainable Management in Amazon, Brazil.
Authors: N Teixeira Fernanda , E Bassil Pamela , C.S Lima Loíse , H Sandes Paula , X Martins Mayara , A.A Salim Ana Paula , B Mano Sérgio , T Mársico Eliane , S Toledo Bruno , Leonardo Kurihara , João Vitor Campos-Silva , C Barros Ana Paula , A.D da Costa Antônio , S Ferreira Micheli
Published: January 2023

The influence of bleeding on color, texture profile, and chemical quality of frozen pirarucu loins were investigated. The loins were obtained from eighteen (n=18) pirarucu carcasses from animals slaughtered by bleeding (BLE; n = 9) or without bleeding (NON-BLE; n = 9). The loins were sliced and assigned randomly for 1 and 12 months at -20ºC to analyze pH, water holding capacity, instrumental color, and texture profile BLE samples exhibited higher L* values (P < 0.05), whereas BLE and NON-BLE exhibited similar (P > 0.05) pH, a* values, b* values, and color stability at months 1 and 12. NON-BLE samples demonstrated greater (P < 0.05) hardness and chewiness at month 1, whereas similar (P > 0.05) springiness and cohesiveness were observed in BLE and NON-BLE samples at months 1 and 12. During storage, Both samples (BLE and NON-BLE) demonstrated a decrease (P < 0.05) in pH and water holding capacity; and an increase (P < 0.05) in lightness, hardness, and chewiness. Also, BLE and NON-BLE exhibited stable (P > 0.05) redness, yellowness, color stability, and cohesiveness. NON-BLE samples exhibited a decrease (P < 0.05) in springiness from the 1st to the 12th month of frozen storage. Bleeding positively influenced the quality of frozen pirarucu.
The Effect of Exercise on the Signs and Symptoms of Inflammatory Bowel Disease.
Authors: Mariah Lund , Max Carlson , Lily Scallon , Sarah Abeln , Mason Lentz , Gary Kastello , Kent Hansen
Published: January 2023

Introduction: Inflammatory Bowel Disease (IBD) generally describes several autoimmune disorders that involve inflammation of the gastrointestinal tract, including Ulcerative Colitis (UC) and Crohn’s disease (CD). An estimated 3.1 million Americans are affected by IBD. Although the inflammation caused by IBD mainly affects the gastrointestinal tract, when IBD is exacerbated, the inflammation can become systemic. Consistent exercise is theorized to reduce chronic inflammation and reduce signs and symptoms of IBD. Objective: This meta-analysis aims to examine the effects of exercise on the signs and symptoms of IBD. Methods: A literary search was conducted using Cochrane Library, PubMed, and Cinahl databases. Of the 1031 records identified, twelve articles met the inclusion criteria, with seven articles presenting data that could be metaanalyzed. The reported data from the seven included articles were standardized to compare dependent variables using the Stata17 statistical package. Pooled effect sizes and 95% confidence intervals were obtained through the random-effects model. The results were used to examine the effects of exercise on C-reactive protein (CRP), fecal calprotectin (FC), and IBD signs and symptoms related questionnaires. Results: Signs and symptoms questionnaires were standardized and compared, resulting in a low risk of heterogeneity of 0.00% (I2), an effect size of 0.02, 95% CI [–0.66 to 0.70], p=0.96. Biomarkers were pooled, standardized, and compared, resulting in heterogeneity of 0.00% (I2) and an effect size of -0.48, 95% CI [–1.60 to 1.42], p=0.40. Conclusions: These results suggest that exercise does not decrease signs and symptoms in IBD patients.
Physicochemical Characteristics and Vitamin A Fortification Levels of Refined Edible Oils Available in the Markets of Ouagadougou, Burkina Faso.
Authors: Dembele Rene , Kambou Mathias , Traore Oumar , Kabore Wendpoulomde Aime Desire , Ouedraogo-Kangambega Marcelline , Soulama Issiaka , Holzinger Mauricio Arcos , Landires Ivan , Kabre Elie , Traore Alfred Sababenedyo , Barro Nicolas
Published: January 2023

Edible oils are vital constituents that provide energy and serve as a carrier of fat-soluble vitamins such as vitamin A. This article aims to evaluate the physicochemical properties and assess the vitamin A content of 45 edible oil samples (37 of palm oil and 8 of cotton seed oil) sold in five markets in Ouagadougou, Burkina Faso. A retinol palmitate standard was used as a control sample for vitamin A content assessment. The physicochemical properties, including the moisture content, acid value, peroxide value, saponification value, and mineral oils content, were determined according to the International Organization for Standardization. Vitamin A levels were assessed by the High-Performance Liquid Chromatography method. The average values of moisture, acid, peroxide contents, and vitamin A were 0.09%, 0.53 mg KOH/g, 6.24 ± 2.92 meq O2/kg, and 6.70 ± 14.26 mg of Vit A/Kg, respectively. Our samples were soap and mineral oils free. However, several samples did not comply with Codex Alimentarius standards for water content (4.44%), acid peroxide values (10%), and vitamin A levels (80%).
A New Homomonoterpenoid of Plectranthus Amboinicus
Authors: YP Zhang Jian , CL Kao , YS Tsai , MD Wu , MJ Cheng , CY Chen , SL Liu
Published: January 2023

A novel homomonoterpenoid, amboinol (1) was isolated from the stems of Plectranthus amboinicus (Lamiaceae). The structure of the new compound was unambiguously elucidated on the basis of extensive spectroscopic?data analysis and comparison with literature data.
Inhibition of Corneal Neovascularization with the Combination of Aflibercept and Plasmid Pigment Epithelium-Derived Factor-Synthetic Amphiphile INTeraction-18 Vector
Authors: TL Yang , CM Liu , HC Yeh , WJ Li , HT Li , CY Chen , SL Liu
Published: January 2023

Aflibercept, a recent VEGF inhibitor, is a recombinant fusion protein consisting of portions of human VEGF receptors 1 and 2 extracellular domains, fused to the Fc portion of human IgG1 formulated as an iso-osmotic solution for intravitreal administration. In this study, we have estimated that the combination of aflibercept and plasmid pigment epithelium-derived factor-synthetic amphiphile INTeraction-18 (p-PEDF-SAINT-18) has a favorable antiangiogenic effect on corneal neovascularization (NV). Four groups (Group A: 0 μg + 0 μg, B: 0. 1μg + 0.1 μg, C: 1 μg + 1 μg, D: 10 μg + 10 μg) of aflibercept + p-PEDF-SAINT-18 were prepared and implanted into rat subconjunctival substantia propria 1.5 mm from the limbus on the temporal side. The 2 μg of p- bFGF-SAINT-18 were prepared and implanted into the rat corneal stroma 1.5mm from the limbus on the same side. Inhibition of NV was observed and quantified from day 1 to day 90. 18-kDa bFGF and VEGF protein expression was analyzed through biomicroscopic examination, western blot analysis and immunohistochemistry. No inhibition activity for normal limbal vessels was noted. Subconjunctival injection by a combination of aflibercept and p-PEDFSAINT-18 successfully inhibited corneal NV. Successful genes of bFGF and PEDF were expressed through western blot analysis; immunohistochemistry staining showed mild immune response for HLA-DR. We conclude that the combination of aflibercept and p-PEDF-SAINT-18 may have more potent and prolonged antiangiogenic effects, making it possible to reduce the frequency of subconjunctival aflibercept administration, while maintaining relatively high safety and low toxicity.
Hypolipidemic Effect of Aqueous Fruit Extract of Doum Palm (Hyphaene Thebaica) in Wistar Rat
Authors: AN Abdullahi , Y Abdulmumin , TM Abdulmumin , SM Sheshe , SY Ismail , M Murtala , AM Ibrahim , MK Hassan , SA Bichi , SI Sarki , S Abubakar
Published: January 2023

Atherosclerotic cardiovascular diseases are characterized by strokes and coronary artery disease. Hyperlipidemia and free radicals generation in the body are among the main risk factors for cardiovascular diseases. Statin drugs such as lovastatin and atorvastatin are mostly prescribed for the treatment of hyperlipidemia. However, many patients show intolerance toward these drugs and develops mild symptoms, which include myalgia and cramps, to the most severe Rhabdomyolysis. In this study, we have qualitatively determined the phytochemicals in Doum (Hyphaene thebaica) fruit aqueous extract and the antioxidant activity using the DPPH assay. Furthermore, we have investigated the hypolipidemic potential of the extract in Wistar rats. Freshly prepared aqueous extract of the Doum fruit with concentration 200 - 400 mg/kg and 20mg/kg of atorvastatin were orally administered to the Wistar rats fed with high - fat diet for two weeks and were sacrificed for hyperlipidemic analysis. The qualitative determination of the phytochemicals indicates the presence of steroids, saponin, tannins, phlobatannins, terpenoids, alkaloid, glycoside, and flavonoids. The fruit extract shows antioxidant activity by scavenging the DPPH radicals with IC50 of 128 µm/mL. Both the fruit extract and atorvastatin decreased serum total glyceride cholesterol, total cholesterol, LDL, and increased HDL level significantly (P < 0.05). This result revealed the anti-hyperlipidemic, and antioxidant potential of Doum fruit. Therefore, it could be useful in managing hyperlipidemia, thereby reducing the risk factor of cardiovascular diseases in the end.
A New Phenylalkanoid of Alpinia Galangal
Authors: Kuo Tang Yu , Chen Hsuan En , Yeh Hung Chun , Chen Chung Yi , Liu Su Ling
Published: January 2023

A new phenylalkanoid, undecyl (E)-8-(4-hydroxy-3-methoxyphenyl)-6- oxooct-7-enoate (1) was isolated from the rhizomes of Alpinia galangal (Zingiberaceae). The structure of the new phenylalkanoid was elucidated by chemical and physical evidence.
Structure, Quantum Chemical and In Silico Molecular Docking Analysis of some Di-Ortho-Substituted Halogenated Biphenyls
Authors: Kumari Neha , Sharma Ruchika , Singh Mulveer , Raj Jayakumar Mohan , Murugavel Saminathan , Sundramoorthy Sonachalam , Kant Rajni
Published: January 2023

The structures of three di-ortho-substituted halogenated biphenyls have been revisited for their optimized geometry and other quantum chemical investigations. The X-ray data, in conjunction with quantum chemical investigations, reveals some interesting results. The Hirshfeld surface analysis helps visualize various intermolecular interactions and the energy frameworks dwell further on the dominant interaction energy component for each structure. To study the inhibitory behaviour of each biphenyl against Cytochrome-P450-14alpha-sterol demethylase fungal enzyme (PDB code: 1EA1), the results of molecular docking studies suggest that the di-ortho-substituted halogenated biphenyls may be regarded as effective and efficient antifungal drugs.
History, Etiology, and Treatment of Paroxysmal Nocturnal Hemoglobinuria
Authors: Graf Michael , Gallicchio Vincent S
Published: January 2023

Paroxysmal nocturnal hemoglobinuria is an acquired, rare, nonmalignant hematological disease that is characterized by uncontrolled complement activation and thus intravascular hemolysis. It is caused by somatic mutations in the PIG-A gene that leads to a deficiency in necessary GPI-anchored proteins, which leads to uncontrolled complement activation and thus the clinical manifestations that PNH is often associated with. It is generally accepted that Dr. Paul Strübing first described the disease in 1882, albeit it was Thomas Hale Ham who designed the first diagnostic test (Ham test or acidified serum test) to diagnose the rare disease. Since then, Ham’s test has become obsolete and PNH is detected via flow cytometry. PNH is divided into 3 categories: classical, presence of another bone marrow failure syndrome, and subclinical. Classic PNH is often associated with bone marrow failure, intravascular hemolysis, and/or thrombophilia. A thromboembolic event is the most common cause of mortality for patients with PNH. Before the introduction of anti-complement drugs, allogeneic hematopoietic stem cell transplantation was a common therapy for patients with PNH, although it carried significant risk and has declined drastically as the preferred treatment option of patients. Presently, there are 3 FDA-approved drugs, two terminal complement inhibitors (eculizumab and ravulizumab) and one proximal complement inhibitor (pegcetacoplan). Eculizumab remains the mainstay of treatment as it has since 2007. There is ongoing research and clinical trials in other proximal complement inhibitors to relieve patients of extravascular hemolysis and other common side effects.
Atrial Septal Defect in Two Nigerian Adults: 2 Case Reports
Authors: Aisosa Ogbomo , O Okorare , John Osarenkhoe Osaretin
Published: January 2023

ASD is one of the most common asymptomatic congenital heart defects manifesting in adulthood. We report two cases managed in a tertiary facility in Nigeria. The first case is a 31-year-old woman who came to the hospital with overt symptoms of heart failure. Color Doppler showed a left to right shunt in the inter-atrial septum (Ostium Primum type). She subsequently improved with anti-failure regimen and anticoagulants. The second case is a 32-year-old man who presented with easy fatigability of four months. Transthoracic echocardiogram revealed a mid-inter-atrial septal defect (Ostium Secundum type) with dilated heart chambers. He is for surgery pending when finances permit.
COVID-19 and Illegal US Bioweapons Activity, an Insider's Revelations
Authors: Bart Classen J
Published: January 2023

Millions if not billions of people now believe that the world is under a bioweapons attack. A lot of blame has been placed on the US funding of bioweapons. Dr. Anthony Fauci, Director of the US NIAID/NIH has come under severe criticism for funding the infamous “Gain of Function” research. Many believe Fauci has not acted alone or even on his own accord. The Author worked as a fellow at NIAID/NIH from 1988 to 1991, starting soon after Fauci became head of NIAID. The author witnessed first hand infiltration of NIAID/NIH by Israel’s Mossad. While working at NIAID/NIH one or more coworkers expressed a need to “preempt the next Jewish Holocaust”. For thirty years since leaving NIH the Author has paid close attention to Mossad’s involvement in illegal US bioweapon activity and attempts to disclose his observations. The Author hopes to educate the public on the current bioweapon attack with COVID-19 and vaccines. The Author believes that Mossad intends to kill billions of people. Already 70% of the world’s population have received a COVID-19 vaccine which in all appearance has the ability to cause deadly non curable prion disease. The Author hopes the information provided will help those interested in ending this covert war.
Pathophysiologic Functions of Carbohydrate-Lipid Interactions: Ascertainment in Obesity, Diabetes and SARS-CoV-2/COVID-19
Authors: Sr Chrysanthus Chukwuma
Published: January 2023

The extant COVID-19 pandemic has resulted in an expansive mortality due to the SARS-CoV-2. The SARS-CoV-2 is encompassed by a lipid bilayer that enhances fusion of the viral membrane to the host cell, replication, endocytosis, exocytosis and role of lipid metabolism in viral infectivity. In the absence of appropriate drugs and vaccines, there are never-ending opportunities for antiviral treatments. Consumption of diets suffused with carbohydrates and saturated fats contribute to obesity and diabetes prevalence, oxidative stress and comorbidities development as risk factors for COVID-19 pandemic as an emergency public health enigma. COVID-19 outbreak has invariably constituted a severe challenge to global public health system with resultant deficient approaches to stem the disorder but carbohydrates may provide accelerated diagnostics, appropriate, effective and efficient vaccines and therapeutic regimen. This paper provides a set of themes and modalities for analysing carbohydrate-lipid interactions as extrapolated to the issues and challenges of SARS-CoV-2, the causative agent of the COVID-19 pandemic.
Synaptic Processes in Raphe Magnus Nucleus under Activation Periaqueductal Gray Matter on the Model of Parkinson’s Disease.
Authors: G.Kh Musheghyan , М.V Poghosyan , A.L Minasyan , H.Y Stepanyan , J.S Sarkissian
Published: January 2023

The electrophysiological studies on 4 Albino rats (230 ± 30?.) on the rotenone model of Parkinson’s disease (PD), induced by unilateral injection of rotenone and kept 4 weeks has been conducted. The extracellular online recording of spike activity of 149 single neurons of the Raphe Magnus (RMG) at high frequency stimulation (HFS) of Periaqueductal gray (PAG) had produced. The programmed mathematical analysis of spikes averaged frequency, with recalculation in interpulse intervals and frequencies to assess of degree of manifestation depressor and excitatory effects as well as the frequency of pre- and post-stimulus activation degree of manifestation in terms of diagrams of averaged frequency spikes are used. On the model of PD a significant reduction in quantity of RMG neurons, evoked at HFS PAG by depressor and depressor–excitatory manifestations of activity had been revealed. On the model of PD, the significant reduction in the number of neurons RMG, responding at PAG HFS by depressor and depressor-excitatory manifestations of activity has been revealed. Analysis of relative degree of intensity of depressor and excitatory effects, by way of example diagrams average frequency of spikes, led to the conclusion. It has been discovered the prevalence of excitatory post stimulus manifestations of activity over depressive (on the order of 1.63- and 2.0-multiple). The pre stimulus frequency of activity substantially prevailed in neurons, responding by excitatory effects (on the order of 2.46- and 17.0-multiple in uni- and multidirectional successions, respectively). As regards to post stimulus frequency, the difference is even higher proved– on the order of 14.6 and even 56.5-multiple for uni- and multidirectional sequences, respectively. Besides, especially larger for multidirectional successions. In other words, under PD excitotoxicity revealed, promoting the powerful frequency growth of pre- and post-stimulus activity in both excitatory sequences, testifying about deep neurodegenerative defeat of important ant nociceptive structure – RMG and promotes the emergence of resistant chronic pain.
Saccharomyces Specific- HCP Assay for r-PAI-2 Therapeutic Proteins.
Authors: Rajasekariah G-Halli
Published: January 2023

Whenever a host cell is employed for a means of propagation of recombinants, some impurities are bound to come across and they are invariably of host cell components and are part of final recombinant proteins. These are investigated for the first time in case of recombinant host, “yeast cells” Saccharomyces. With great care, high affinity antibodies are generated for yeast proteins using Gene-minus (null-cell) yeast components purified by down-stream techniques with appropriate resins for column purification and these antibodies are being used to construct specific sandwich ELISA system to monitor the degree of host contaminants proteins (HCPs). How this is being done and what strategic methods are followed and how the HCP- detection ELISA systems are constructed and what are the limitations?. These points are described and discussed along with prospects of HCPs in the intensified therapeutic proteins preparation. Our HCP assay is sensitive enough to detect 200-250 ppm in the final PAI-2 product.
Fenntartható-e a jelenlegi magyarországi népességszám a megváltozott gyermekvállalási preferenciák mellett?
Authors: Drabancz Áron , Berde Éva
Published: January 2023

Tanulmányunkban áttekintjük mely főbb gazdasági, kulturális és társadalmi folyamatok járultak hozzá a teljes termékenységi arányszám csökkenéséhez a fejlett országokban. A szakirodalmi áttekintés eredményei szerint ezen tendenciák a jövőben is fennállhatnak vagy még tovább erősödhetnek, így a teljes termékenységi arányszám növekedése hosszabb távon sem valószínű. Mikroökonómiai modellkeretben mutatjuk be, hogy a gyermek határköltségétől függetlenül a kisebb gyermekszámú családok válnak egyre inkább ideálissá, amennyiben a gyermek határhaszna a szülők számára nagymértékben csökken. Nemzetközi összevetés eredményei alapján arra jutottunk, hogy a Magyarországon az elmúlt években megfigyelt növekvő teljes termékenységi arányszám nagyrészt a halasztási magatartás lelassulásából következett, és az arányszám emelkedése a régió legtöbb országában megfigyelhető volt. A családtámogatási programok változása ezt a folyamatot kevésbé befolyásolta. Emiatt a közeljövőben az elmúlt évtized kedvező tendenciái megfordulhatnak, a teljes termékenységi arányszám akár újra csökkenhet Magyarországon. Ezt erősíti az is, hogy a 2000-es évek elején megfigyelt pozitív korreláció a fertilitás és az egy főre jutó GDP között a fejlett országokban jelentősen csökkent, a legfrissebb adatok tükrében az Európai Unióban ez teljesen el is tűnt. A megváltozott preferenciákkal nem szembe menve így érdemes végiggondolni az erőforrások újracsoportosítását, az elöregedő társadalom kihívásaihoz történő rugalmasabb alkalmazkodást.
Lengyelország meghatározó befolyása Kelet-Közép-Európán belül
Authors: Tamás Szintia
Published: January 2023

Kelet-Közép-Európában Lengyelország igen fontos szerepet tölt be a gazdaságban. A tanulmány középpontjában Lengyelország meghatározó befolyásának vizsgálata áll a kelet-közép-európai térségen belül. Több mint egy évszázada meghatározza a lengyel külpolitika irányát az ország földrajzi helyzete, valamint a nagyhatalmak közé való ékeltsége. A tanulmány célja a lengyel külpolitikai irányultság elemzése és bemutatása. A tanulmány hat részre oszlik. Az első részben bemutatom a kutatás elméleti keretrendszerét, a második részben pedig a tanulmány módszertanát. A kutatásom harmadik részében Lengyelország jelenlegi helyzetének történelmi előzményeit mutatom be. Ezt követően Lengyelország szerepét ismertetem az 1990-es években, illetve a lengyel-orosz kapcsolatokat, amelyen belül részletesen bemutatom az orosz-ukrán konfliktust is. Végül a lengyel energetikapolitikát vizsgálom meg, majd pedig a tanulmány végén a Lengyelország meghatározó befolyásáról megfogalmazott következtetések zárnak. A tanulmány kutatási módszere a társadalomtudományokban leggyakrabban alkalmazott kutatási design mentén épül föl: korábbi elméleti eredményekre építő elméleti keret felvázolása, a főbb elméleti és empirikus irodalmak összegzése, amelyet a hipotézisek meghatározása követ, majd a kutatási kérdések operacionalizálása, illetve a vizsgálati dimenziók kialakítása, legvégül pedig a konklúziók levonása. Lengyelország szerepe egyre nem csak a kelet-közép-európai régión belül jelentősebb, hanem globálisan is. Egyrészről elsődleges segítséget nyújthat a jelenlegi orosz agresszió ellen Ukrajnából menekülő személyek számára, valamint támogathatja az Európai Unió által nyújtott humanitárius segélynyújtások sikerességét. Másrészről pedig a Norvégiával ápolt kiváló kapcsolata által jó üzleti partner és közvetítő lehet a kelet-közép-európai országok számára az energetikapolitika kérdéseiben.
Urinary Bladder Stone: A Late Complication of Bulkamid® Periurethral Bulking Injection
Authors: Uwadiae Harmony , Pai Aakash , Al-Singary Waleed
Published: January 2023

Polyacrylamide hydrogel bulking agent (Bulkamid® [Axonics, Irvine, California, USA]) injection is used as a minimally invasive treatment for stress and mixed urinary incontinence in females. Several studies have demonstrated the short- and medium-term efficacy and safety of Bulkamid. However, there are limited data available on the long-term safety of this procedure. The authors report an unexpected and late complication associated with Bulkamid periurethral injection.

An 80-year-old female, who had undergone Bulkamid periurethral injection for stress urinary incontinence 10 years previously, was referred to the authors’ clinic with recurrent lower urinary tract symptoms and dysuria. Investigations with ultrasound and cystoscopy confirmed a single 2 cm bladder stone adhered to an exposed Bulkamid agent at its injection site.

Exposed intravesical Bulkamid can act as a foreign body with lithogenic potential to cause urinary bladder stone formation. This article highlights urinary bladder stone formation as a late potential complication of Bulkamid periurethral injection.
Recovering Classical Liberal Political Economy
Authors: Ward Lee
Published: January 2023

This book reexamines the philosophical roots of classical liberal political economy. It proposes an interpretive model based upon the interconnection between distinct theories of natural rights and the harmony of interests. It takes a fresh look at classical liberalism by exploring economic arguments in thinkers like Thomas Hobbes, Thomas Paine, John Trenchard and Thomas Gordon, who are not typically viewed as economic thinkers, and by highlighting the importance of Bernard Mandeville and Adam Smith in the development of interest-based liberalism. It also re-examines lesser-known economic tracts by thinkers such as John Locke, David Hume, and John Stuart Mill in light of their more well-known political writings. This book also sheds light on how classical liberal political economists struggled, largely unsuccessfully, to grapple with the injustices of imperialism and the subjection of women. With classical liberal assumptions still prominent in contemporary debates about economic justice, it is vital for every democratic citizen to understand the complex origins and development of the ideas that did so much to shape our world today.
Bantu Presbyterian Church of South Africa
Authors: Duncan Graham A.
Published: January 2023

This book gives an account of the Free Church of Scotland mission to South Africa from its beginning in 1824. It explains the progress of mission up to the formation of the Bantu Presbyterian Church of South Africa (BPCSA) in 1923 and its subsequent history, through its name change to the Reformed Presbyterian Church in Southern Africa in 1979, until it united with the Presbyterian Church of Southern Africa in 1999 to form the Uniting Presbyterian Church in Southern Africa. It considers the reasons for the delay in forming an autonomous church by emphasising the role of mission councils which was out of proportion to their long term value. This delayed the development of indigenous leadership The history of the BPCSA is viewed in the context of the periods of segregation and apartheid in South Africa and in the context of its ecumenical commitment. Attention is given to the involvement of the BPCSA in socio-political issues and the role of women in the church is examined.
Rumi
Authors: Renard John
Published: January 2023

Picturing the life story of Jalal ad-Din Rumi, a premier Muslim mystic (Sufi) and the original Whirling Dervish, the images in three extant manuscripts of Aflaki’s Wondrous Feats of the Knowers of God provide a unique way to interpret the text. Part One: History and Context provides the medieval Anatolian historical setting; the broad contours of literary and artistic works of Islamic Hagiography; and the specific details of the three manuscripts to be explored. Part Two: Text and Image proposes a method for interpreting a hybrid literary–visual document as a grand narrative of the Family Rumi at the inspirational and ethical core of a virtuous community – flourishing within a complex Muslim society under divine providence. Included are 68 full-colour full-page images, gathering for the first time all of the paintings from the three manuscripts, accompanied by a summary of the text illustrated in each picture. Analysis of the images focuses on the specific relationships between literary narrative and its visual representation in late medieval Islamic art. The images in the three manuscripts were produced by studios of painters under the patronage of major late 16th-century Ottoman sultans. The result of their efforts is a kind of ‘visualised hagiography’ uniquely capable of suggesting distinctive and often surprising twists on the narratives, enhancing the text with images of striking beauty and rich detail. [221]
Counterpoetics of Modernity
Authors: Lloyd David
Published: January 2023

This study puts contemporary Irish poetry in dialogue with major debates and concerns of European and American poetics. David Lloyd tracks the traits of Irish poetic modernism, from fragmentation to the suspicion of representation, to nineteenth-century responses to the rapid and unsettling effects of Ireland’s precocious colonial modernity, such as language loss and political violence. He argues that Irish poetry’s inventiveness is driven by the need to find formal means to engage with historical conditions that take from the writer the customary certainties of cultural continuity, identity and aesthetic or personal autonomy, rather than by poetic innovation for its own sake. This reading of Irish poetry understands the innovative impetus that persists through Irish poetry since the 19th century as a counterpoetics of modernity. Opening with chapters on Mangan and Yeats, the book then turns to detailed discussions of major Irish contemporary poets never before the focus of a book-length study: Trevor Joyce, Maurice Scully, and Catherine Walsh.
Reforming the Law of Nature
Authors: Kennedy Simon P.
Published: January 2023

This book is a stimulating study of the development of natural law ideas in the early modern period, uncovering their connection to conceptions of the origins of politics. It brings sixteenth and seventeenth century jurisprudence, theology and political philosophy into conversation with one another to uncover the ways in which developments in political thought affected the emergence of a secular understanding of political life. The book covers the development of ideas from the early Reformed tradition, with close examinations of John Calvin, Richard Hooker and Johannes Althusius. The final chapters show how the Reformed understanding of natural law was fundamentally altered in the latter part of the seventeenth century, paving the way for secularised conceptions of politics. The figures of Thomas Hobbes and John Locke are exemplars of this change. Whereas earlier understandings of natural law provided a theistic and Aristotelian naturalistic basis for the origins of political life, thinkers like Hobbes and Locke severed the connection between God and human politics by reshaping the origins and purpose of natural law.
Pastoral conflicts and (dis)trust: Evidence from Nigeria using an instrumental variable approach
Authors: Tuki Daniel
Published: January 2023

Although the incidence of conflicts between Fulani nomadic pastoralists and sedentary farmers in Nigeria have risen significantly during the last decade, no study has examined how these conflicts influence distrust of members of the Fulani ethnic group and the larger Muslim population, nor the conditions under which these conflicts, which are primarily about competition over land and water resources, morph into religious conflicts. Using novel survey data collected from Kaduna, the state with the third highest incidence of pastoral conflicts in Nigeria, this study fills these gaps. The regression results show that exposure to pastoral conflicts cause distrust of members of the Fulani ethnic group and Muslims; although the size of the effect is much larger for the Fulani compared to Muslims. This shows that the population in Kaduna tend to conflate the Fulani with Muslims. Religious polarization was found to catalyze the process of resource conflicts turning religious.
CE2 ASPA TLC PowerPoint Introduction
Authors: LAWRENCE STEVEN
Published: January 2023

Introduction to CE2 at Walters State. A successful small grant funded project A series of student-led public civics classes Free on-campus for any member of our multi-county area …who are interested in learning more about how our government works.
Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices
Authors: Fok Shierly W. , Gresham Robert C. H. , Ryan Weston , Osipov Benjamin , Bahney Chelsea , Leach J. Kent
Published: January 2023

Recombinant growth factors are used in tissue engineering to stimulate cell proliferation, migration, and differentiation. Conventional methods of growth factor delivery for therapeutic applications employ large amounts of these bioactive cues. Effective, localized growth factor release is essential to reduce the required dose and potential deleterious effects. The endogenous extracellular matrix (ECM) sequesters native growth factors through its negatively charged sulfated glycosaminoglycans. Mesenchymal stromal cells secrete an instructive extracellular matrix that can be tuned by varying culture and decellularization methods. In this study, mesenchymal stromal cell-secreted extracellular matrix was modified using λ-carrageenan as a macromolecular crowding (MMC) agent and decellularized with DNase as an alternative to previous decellularized extracellular matrices (dECM) to improve growth factor retention. Macromolecular crowding decellularized extracellular matrix contained 7.7-fold more sulfated glycosaminoglycans and 11.7-fold more total protein than decellularized extracellular matrix, with no significant difference in residual DNA. Endogenous BMP-2 was retained in macromolecular crowding decellularized extracellular matrix, whereas BMP-2 was not detected in other extracellular matrices. When implanted in a murine muscle pouch, we observed increased mineralized tissue formation with BMP-2-adsorbed macromolecular crowding decellularized extracellular matrix in vivo compared to conventional decellularized extracellular matrix. This study demonstrates the importance of decellularization method to retain endogenous sulfated glycosaminoglycans in decellularized extracellular matrix and highlights the utility of macromolecular crowding to upregulate sulfated glycosaminoglycan content. This platform has the potential to aid in the delivery of lower doses of BMP-2 or other heparin-binding growth factors in a tunable manner.
Regulating Early Biological Events in Human Amniotic Epithelial Stem Cells Using Natural Bioactive Compounds: Extendable Multidirectional Research Avenues
Authors: Ferdousi Farhana , Isoda Hiroko
Published: January 2023

Graphical Abstract
Spermine oxidase induces DNA damage and sensitizes fusion negative rhabdomyosarcoma cells to irradiation
Authors: Perrone Clara , Pomella Silvia , Cassandri Matteo , Pezzella Michele , Giuliani Stefano , Gasperi Tecla , Porrazzo Antonella , Alisi Anna , Pastore Anna , Codenotti Silvia , Fanzani Alessandro , Barillari Giovanni , Conti Libenzio Adrian , De Angelis Biagio , Quintarelli Concetta , Mariottini Paolo , Locatelli Franco , Marampon Francesco , Rota Rossella , Cervelli Manuela
Published: January 2023

Rhabdomyosarcoma (RMS) is a pediatric myogenic soft tissue sarcoma that includes fusion-positive (FP) and fusion-negative (FN) molecular subtypes. FP-RMS expresses PAX3-FOXO1 fusion protein and often shows dismal prognosis. FN-RMS shows cytogenetic abnormalities and frequently harbors RAS pathway mutations. Despite the multimodal heavy chemo and radiation therapeutic regimens, high risk metastatic/recurrent FN-RMS shows a 5-year survival less than 30% due to poor sensitivity to chemo-radiotherapy. Therefore, the identification of novel targets is needed. Polyamines (PAs) such as putrescine (PUT), spermidine (SPD) and spermine (SPM) are low-molecular-mass highly charged molecules whose intracellular levels are strictly modulated by specific enzymes. Among the latter, spermine oxidase (SMOX) regulates polyamine catabolism oxidizing SPM to SPD, which impacts cellular processes such as apoptosis and DNA damage response. Here we report that low SMOX levels are associated with a worse outcome in FN-RMS, but not in FP-RMS, patients. Consistently, SMOX expression is downregulated in FN-RMS cell lines as compared to normal myoblasts. Moreover, SMOX transcript levels are reduced FN-RMS cells differentiation, being indirectly downregulated by the muscle transcription factor MYOD. Noteworthy, forced expression of SMOX in two cell lines derived from high-risk FN-RMS: 1) reduces SPM and upregulates SPD levels; 2) induces G0/G1 cell cycle arrest followed by apoptosis; 3) impairs anchorage-independent and tumor spheroids growth; 4) inhibits cell migration; 5) increases γH2AX levels and foci formation indicative of DNA damage. In addition, forced expression of SMOX and irradiation synergize at activating ATM and DNA-PKCs, and at inducing γH2AX expression and foci formation, which suggests an enhancement in DNA damage response. Irradiated SMOX-overexpressing FN-RMS cells also show significant decrease in both colony formation capacity and spheroids growth with respect to single approaches. Thus, our results unveil a role for SMOX as inhibitor of tumorigenicity of FN-RMS cells in vitro. In conclusion, our in vitro results suggest that SMOX induction could be a potential combinatorial approach to sensitize FN-RMS to ionizing radiation and deserve further in-depth studies.
A glycolysis-related gene signatures in diffuse large B-Cell lymphoma predicts prognosis and tumor immune microenvironment
Authors: Cui Yingying , Leng Changsen
Published: January 2023

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma which that highly aggressive and heterogeneous. Glycolysis has been implicated in the regulation of tumor microenvironment (TME) and development. In this study, we aimed to establish a glycolysis-related prognostic model for the risk stratification, prognosis prediction, and immune landscape evaluation in patients with DLBCL.

Methods: Three independent datasets GSE181063, GSE10846, and GSE53786 containing gene expression profiles and clinical data were downloaded from the Gene Expression Omnibus (GEO) database. The glycolysis-related prognostic model was developed with Cox and Least Absolute Shrinkage and Selector Operation (LASSO) regression and validated. A nomogram integrating clinical factors and glycolytic risk scores was constructed. The composition of the TME was analyzed with the ESTIMATE algorithm and single-sample gene set enrichment analysis (ssGSEA).

Results: A glycolytic risk model containing eight genes was developed. The area under the receiver operating characteristic (ROC) curve (AUC) for the 1-, 3-, and 5-year was 0.718, 0.695, and 0.688, respectively. Patients in the high-risk group had significantly lower immune scores, elevated tumor purity, and poorer survival compared with those in the low-risk group. The nomogram constructed based on glycolytic risk score, age, Eastern Cooperative Oncology Group performance status (ECOG-PS), use of rituximab, and cell of origin (COO) displayed better prediction performance compared with the International Prognostic Index (IPI) in DLBCL. The glycolytic risk score was negatively correlated with the infiltration level of activated CD8 T cells, activated dendritic cells, natural killer cells, and macrophages and immune checkpoint molecules including PD-L2, CTLA4, TIM-3, TIGIT, and B7-H3.

Conclusion: These results suggested that the glycolytic risk model could accurately and stably predict the prognosis of patients with DLBCL and might unearth the possible explanation for the glycolysis-related poor prognosis.