bluuebunny/crossref_metadata_2025 huggingface dataset:
Dissecting Response to Cancer Immunotherapy by Applying Bayesian Network Analysis to Flow Cytometry Data
Authors: Rodin Andrei S. , Gogoshin Grigoriy , Hilliard Seth , Wang Lei , Egelston Colt , Rockne Russell C. , Chao Joseph , Lee Peter P.
Published: February 2021

Cancer immunotherapy, specifically immune checkpoint blockade, has been found to be effective in the treatment of metastatic cancers. However, only a subset of patients achieve clinical responses. Elucidating pretreatment biomarkers predictive of sustained clinical response is a major research priority. Another research priority is evaluating changes in the immune system before and after treatment in responders vs. nonresponders. Our group has been studying immune networks as an accurate reflection of the global immune state. Flow cytometry (FACS, fluorescence-activated cell sorting) data characterizing immune cell panels in peripheral blood mononuclear cells (PBMC) from gastroesophageal adenocarcinoma (GEA) patients were used to analyze changes in immune networks in this setting. Here, we describe a novel computational pipeline to perform secondary analyses of FACS data using systems biology/machine learning techniques and concepts. The pipeline is centered around comparative Bayesian network analyses of immune networks and is capable of detecting strong signals that conventional methods (such as FlowJo manual gating) might miss. Future studies are planned to validate and follow up the immune biomarkers (and combinations/interactions thereof) associated with clinical responses identified with this computational pipeline.
Synthesis of Novel Pyrido[1,2-c]pyrimidine Derivatives with 6-Fluoro-3-(4-piperidynyl)-1,2-benzisoxazole Moiety as Potential SSRI and 5-HT1A Receptor Ligands
Authors: Król Marek , Ślifirski Grzegorz , Kleps Jerzy , Ulenberg Szymon , Belka Mariusz , Bączek Tomasz , Siwek Agata , Stachowicz Katarzyna , Szewczyk Bernadeta , Nowak Gabriel , Duszyńska Beata , Herold Franciszek
Published: February 2021

Two series of novel 4-aryl-2H-pyrido[1,2-c]pyrimidine (6a–i) and 4-aryl-5,6,7,8-tetrahydropyrido[1,2-c]pyrimidine (7a–i) derivatives were synthesized. The chemical structures of the new compounds were confirmed by 1H and 13C NMR spectroscopy and ESI-HRMS spectrometry. The affinities of all compounds for the 5-HT1A receptor and serotonin transporter protein (SERT) were determined by in vitro radioligand binding assays. The test compounds demonstrated very high binding affinities for the 5-HT1A receptor of all derivatives in the series (6a–i and 7a–i) and generally low binding affinities for the SERT protein, with the exception of compounds 6a and 7g. Extended affinity tests for the receptors D2, 5-HT2A, 5-HT6 and 5-HT7 were conducted with regard to selected compounds (6a, 7g, 6d and 7i). All four compounds demonstrated very high affinities for the D2 and 5-HT2A receptors. Compounds 6a and 7g also had high affinities for 5-HT7, while 6d and 7i held moderate affinities for this receptor. Compounds 6a and 7g were also tested in vivo to identify their functional activity profiles with regard to the 5-HT1A receptor, with 6a demonstrating the activity profile of a presynaptic agonist. Metabolic stability tests were also conducted for 6a and 6d.
The Emerging Role of Macrophages in Immune System Dysfunction under Real and Simulated Microgravity Conditions
Authors: Sun Yulong , Kuang Yuanyuan , Zuo Zhuo
Published: February 2021

In the process of exploring space, the astronaut’s body undergoes a series of physiological changes. At the level of cellular behavior, microgravity causes significant alterations, including bone loss, muscle atrophy, and cardiovascular deconditioning. At the level of gene expression, microgravity changes the expression of cytokines in many physiological processes, such as cell immunity, proliferation, and differentiation. At the level of signaling pathways, the mitogen-activated protein kinase (MAPK) signaling pathway participates in microgravity-induced immune malfunction. However, the mechanisms of these changes have not been fully elucidated. Recent studies suggest that the malfunction of macrophages is an important breakthrough for immune disorders in microgravity. As the first line of immune defense, macrophages play an essential role in maintaining homeostasis. They activate specific immune responses and participate in large numbers of physiological activities by presenting antigen and secreting cytokines. The purpose of this review is to summarize recent advances on the dysfunction of macrophages arisen from microgravity and to discuss the mechanisms of these abnormal responses. Hopefully, our work will contribute not only to the future exploration on the immune system in space, but also to the development of preventive and therapeutic drugs against the physiological consequences of spaceflight.
Transcriptomic Changes Associated with Loss of Cell Viability Induced by Oxysterol Treatment of a Retinal Photoreceptor-Derived Cell Line: An In Vitro Model of Smith–Lemli–Opitz Syndrome
Authors: Pfeffer Bruce A. , Xu Libin , Fliesler Steven J.
Published: February 2021

Smith–Lemli–Opitz Syndrome (SLOS) results from mutations in the gene encoding the enzyme DHCR7, which catalyzes conversion of 7-dehydrocholesterol (7DHC) to cholesterol (CHOL). Rats treated with a DHCR7 inhibitor serve as a SLOS animal model, and exhibit progressive photoreceptor-specific cell death, with accumulation of 7DHC and oxidized sterols. To understand the basis of this cell type specificity, we performed transcriptomic analyses on a photoreceptor-derived cell line (661W), treating cells with two 7DHC-derived oxysterols, which accumulate in tissues and bodily fluids of SLOS patients and in the rat SLOS model, as well as with CHOL (negative control), and evaluated differentially expressed genes (DEGs) for each treatment. Gene enrichment analysis and compilation of DEG sets indicated that endoplasmic reticulum stress, oxidative stress, DNA damage and repair, and autophagy were all highly up-regulated pathways in oxysterol-treated cells. Detailed analysis indicated that the two oxysterols exert their effects via different molecular mechanisms. Changes in expression of key genes in highlighted pathways (Hmox1, Ddit3, Trib3, and Herpud1) were validated by immunofluorescence confocal microscopy. The results extend our understanding of the pathobiology of retinal degeneration and SLOS, identifying potential new druggable targets for therapeutic intervention into these and other related orphan diseases.
Natural Products Targeting Amyloid Beta in Alzheimer’s Disease
Authors: Lee Joo-Hee , Ahn Na-Hyun , Choi Su-Bin , Kwon Youngeun , Yang Seung-Hoon
Published: February 2021

Alzheimer’s disease (AD) is a neurodegenerative disease characterized by severe brain damage and dementia. There are currently few therapeutics to treat this disease, and they can only temporarily alleviate some of the symptoms. The pathogenesis of AD is mainly preceded by accumulation of abnormal amyloid beta (Aβ) aggregates, which are toxic to neurons. Therefore, modulation of the formation of these abnormal aggregates is strongly suggested as the most effective approach to treat AD. In particular, numerous studies on natural products associated with AD, aiming to downregulate Aβ peptides and suppress the formation of abnormal Aβ aggregates, thus reducing neural cell death, are being conducted. Generation of Aβ peptides can be prevented by targeting the secretases involved in Aβ-peptide formation (secretase-dependent). Additionally, blocking the intra- and intermolecular interactions of Aβ peptides can induce conformational changes in abnormal Aβ aggregates, whereby the toxicity can be ameliorated (structure-dependent). In this review, AD-associated natural products which can reduce the accumulation of Aβ peptides via secretase- or structure-dependent pathways, and the current clinical trial states of these products are discussed.
Study of the Cross-Influence between III-V and IV Elements Deposited in the Same MOVPE Growth Chamber
Authors: Timò Gianluca , Calicchio Marco , Abagnale Giovanni , Armani Nicola , Achilli Elisabetta , Cornelli Marina , Annoni Filippo , Schineller Bernd , Andreani Lucio Claudio
Published: February 2021

We have deposited Ge, SiGe, SiGeSn, AlAs, GaAs, InGaP and InGaAs based structures in the same metalorganic vapor phase epitaxy (MOVPE) growth chamber, in order to study the effect of the cross influence between groups IV and III-V elements on the growth rate, background doping and morphology. It is shown that by adopting an innovative design of the MOVPE growth chamber and proper growth condition, the IV elements growth rate penalization due to As “carry over” can be eliminated and the background doping level in both IV and III-V semiconductors can be drastically reduced. In the temperature range 748–888 K, Ge and SiGe morphologies do not degrade when the semiconductors are grown in a III-V-contaminated MOVPE growth chamber. Critical morphology aspects have been identified for SiGeSn and III-Vs, when the MOVPE deposition takes place, respectively, in a As or Sn-contaminated MOVPE growth chamber. III-Vs morphologies are influenced by substrate type and orientation. The results are promising in view of the monolithic integration of group-IV with III-V compounds in multi-junction solar cells.
Antibiofilm and Protein-Repellent Polymethylmethacrylate Denture Base Acrylic Resin for Treatment of Denture Stomatitis
Authors: Bajunaid Salwa O. , Baras Bashayer H. , Balhaddad Abdulrahman A. , Weir Michael D. , Xu Hockin H. K.
Published: February 2021

Candida albicans (C. albicans) biofilm is a common etiological factor in denture stomatitis. The purpose of this study was to investigate the effects of incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) as a protein repellent into a new high-impact denture acrylic (HIPA) resin on the surface roughness, solution pH, and C. albicans biofilm adhesion to the denture base. The new acrylic denture resin base was formulated by mixing MPC into HIPA resin at mass fractions of 1.5%, 3%, and 4.5%. Surface roughness was measured using a Mitutoyo surface roughness tester. C. albicans biofilm growth and viability were assessed via colony forming unit counts. The pH of the biofilm growth medium was measured using a digital pH meter. Adding MPC to the HIPA resin at percentages of 1.5% and 3% increased the roughness values significantly (p < 0.05), while adding 4.5% MPC resulted in no difference in roughness values to that of the control group (p > 0.05). All experimental groups demonstrated neutral pH values (pH ≅ 7) and were not significantly different from each other (p > 0.05). Incorporating 2-methacryloyloxyethyl phosphorylcholine at 4.5% resulted in a significant (≅1 log) colony-forming unit reduction compared with the control group with 0% MPC (p < 0.05). A fungal-retarding denture acrylic resin was developed through the incorporation of MPC for its protein-repelling properties. This newly developed denture acrylic material has the potential to prevent oral microbial infections, such as denture stomatitis.
Comprehensive Machine Learning-Based Model for Predicting Compressive Strength of Ready-Mix Concrete
Authors: Xu Jiajia , Zhou Li , He Ge , Ji Xu , Dai Yiyang , Dang Yagu
Published: February 2021

Considering that compressive strength (CS) is an important mechanical property parameter in many design codes, in order to ensure structural safety, concrete CS needs to be tested before application. However, conducting CS tests with multiple influencing variables is costly and time-consuming. To address this issue, a machine learning-based modeling framework is put forward in this work to evaluate the concrete CS under complex conditions. The influential factors of this process are systematically categorized into five aspects: man, machine, material, method and environment (4M1E). A genetic algorithm (GA) was applied to identify the most important influential factors for CS modeling, after which, random forest (RF) was adopted as the modeling algorithm to predict the CS from the selected influential factors. The effectiveness of the proposed model was tested on a case study, and the high Pearson correlation coefficient (0.9821) and the low mean absolute percentage error and delta (0.0394 and 0.395, respectively) indicate that the proposed model can deliver accurate and reliable results.
Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
Authors: Waletzko-Hellwig Janine , Saemann Michael , Schulze Marko , Frerich Bernhard , Bader Rainer , Dau Michael
Published: February 2021

One main disadvantage of commercially available allogenic bone substitute materials is the altered mechanical behavior due to applied material processing, including sterilization methods like thermal processing or gamma irradiation. The use of high hydrostatic pressure (HHP) might be a gentle alternative to avoid mechanical alteration. Therefore, we compressed ground trabecular human bone to granules and, afterwards, treated them with 250 and 300 MPa for 20 and 30 min respectively. We characterized the formed bone granule cylinders (BGC) with respect to their biomechanical properties by evaluating stiffness and stress at 15% strain. Furthermore, the stiffness and yield strength of HHP-treated and native human trabecular bone cylinders (TBC) as control were evaluated. The mechanical properties of native vs. HHP-treated TBCs as well as HHP-treated vs. untreated BGCs did not differ, independent of the applied HHP magnitude and duration. Our study suggests HHP treatment as a suitable alternative to current processing techniques for allogenic bone substitutes since no negative effects on mechanical properties occurred.
Effect of UV Irradiation and TiO2-Photocatalysis on Airborne Bacteria and Viruses: An Overview
Authors: Bono Nina , Ponti Federica , Punta Carlo , Candiani Gabriele
Published: February 2021

Current COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has put a spotlight on the spread of infectious diseases brought on by pathogenic airborne bacteria and viruses. In parallel with a relentless search for therapeutics and vaccines, considerable effort is being expended to develop ever more powerful technologies to restricting the spread of airborne microorganisms in indoor spaces through the minimization of health- and environment-related risks. In this context, UV-based and photocatalytic oxidation (PCO)-based technologies (i.e., the combined action of ultraviolet (UV) light and photocatalytic materials such as titanium dioxide (TiO2)) represent the most widely utilized approaches at present because they are cost-effective and ecofriendly. The virucidal and bactericidal effect relies on the synergy between the inherent ability of UV light to directly inactivate viral particles and bacteria through nucleic acid and protein damages, and the production of oxidative radicals generated through the irradiation of the TiO2 surface. In this literature survey, we draw attention to the most effective UV radiations and TiO2-based PCO technologies available and their underlying mechanisms of action on both bacteria and viral particles. Since the fine tuning of different parameters, namely the UV wavelength, the photocatalyst composition, and the UV dose (viz, the product of UV light intensity and the irradiation time), is required for the inactivation of microorganisms, we wrap up this review coming up with the most effective combination of them. Now more than ever, UV- and TiO2-based disinfection technologies may represent a valuable tool to mitigate the spread of airborne pathogens.
A Sequence of Phase Transformations and Phases in NiCoFeCrGa High Entropy Alloy
Authors: Vida Ádám , Lábár János , Dankházi Zoltán , Maksa Zsolt , Molnár Dávid , Varga Lajos K. , Kalácska Szilvia , Windisch Márk , Huhn Gabriella , Chinh Nguyen Q.
Published: February 2021

The present investigation is directed to phase transitions in the equimolar NiCoFeCrGa high entropy alloy, which is a mixture of face-centered cubic (FCC) and body-centered cubic (BCC) crystalline phases. The microstructure of the samples was investigated by using scanning electron microscopy (SEM), time-of-flight secondary ion mass spectroscopy (TOF-SIMS), transmission electron microscopy-based energy-dispersive spectroscopy (EDS) and electron energy loss spectroscopy (EELS), as well as X-ray diffraction (XRD) measurements. Based on the phases observed in different temperature ranges, a sequence of the phase transitions can be established, showing that in a realistic process, when freely cooling the sample with the furnace from high to room temperature, a microstructure having spinodal-like decomposition can also be expected. The elemental mapping and magnetic behaviors of this decomposed structure are also studied.
Role of Sintering Temperature in Production of Nepheline Ceramics-Based Geopolymer with Addition of Ultra-High Molecular Weight Polyethylene
Authors: Ahmad Romisuhani , Abdullah Mohd Mustafa Al Bakri , Ibrahim Wan Mastura Wan , Hussin Kamarudin , Ahmad Zaidi Fakhryna Hannanee , Chaiprapa Jitrin , Wysłocki Jerzy J. , Błoch Katarzyna , Nabiałek Marcin
Published: February 2021

The primary motivation of developing ceramic materials using geopolymer method is to minimize the reliance on high sintering temperatures. The ultra-high molecular weight polyethylene (UHMWPE) was added as binder and reinforces the nepheline ceramics based geopolymer. The samples were sintered at 900 °C, 1000 °C, 1100 °C, and 1200 °C to elucidate the influence of sintering on the physical and microstructural properties. The results indicated that a maximum flexural strength of 92 MPa is attainable once the samples are used to be sintered at 1200 °C. It was also determined that the density, porosity, volumetric shrinkage, and water absorption of the samples also affected by the sintering due to the change of microstructure and crystallinity. The IR spectra reveal that the band at around 1400 cm−1 becomes weak, indicating that sodium carbonate decomposed and began to react with the silica and alumina released from gels to form nepheline phases. The sintering process influence in the development of the final microstructure thus improving the properties of the ceramic materials.
Stem Cell-Engineered Nanovesicles Exert Proangiogenic and Neuroprotective Effects
Authors: Kim Han Young , Bhang Suk Ho
Published: February 2021

As a tissue regeneration strategy, the utilization of mesenchymal stem cells (MSCs) has drawn considerable attention. Comprehensive research using MSCs has led to significant preclinical or clinical outcomes; however, improving the survival rate, engraftment efficacy, and immunogenicity of implanted MSCs remains challenging. Although MSC-derived exosomes were recently introduced and reported to have great potential to replace conventional MSC-based therapeutics, the poor production yield and heterogeneity of exosomes are critical hurdles for their further applications. Herein, we report the fabrication of exosome-mimetic MSC-engineered nanovesicles (MSC-NVs) by subjecting cells to serial extrusion through filters. The fabricated MSC-NVs exhibit a hydrodynamic size of ~120 nm, which is considerably smaller than the size of MSCs (~30 μm). MSC-NVs contain both MSC markers and exosome markers. Importantly, various therapeutic growth factors originating from parent MSCs are encapsulated in the MSC-NVs. The MSC-NVs exerted various therapeutic effects comparable to those of MSCs. They also significantly induced the angiogenesis of endothelial cells and showed neuroprotective effects in damaged neuronal cells. The results collectively demonstrate that the fabricated MSC-NVs can serve as a nanosized therapeutic agent for tissue regeneration.
Ion-Imprinted Polymers: Synthesis, Characterization, and Adsorption of Radionuclides
Authors: Kusumkar Vipul Vilas , Galamboš Michal , Viglašová Eva , Daňo Martin , Šmelková Jana
Published: February 2021

Growing concern over the hazardous effect of radionuclides on the environment is driving research on mitigation and deposition strategies for radioactive waste management. Currently, there are many techniques used for radionuclides separation from the environment such as ion exchange, solvent extraction, chemical precipitation and adsorption. Adsorbents are the leading area of research and many useful materials are being discovered in this category of radionuclide ion separation. The adsorption technologies lack the ability of selective removal of metal ions from solution. This drawback is eliminated by the use of ion-imprinted polymers, these materials having targeted binding sites for specific ions in the media. In this review article, we present recently published literature about the use of ion-imprinted polymers for the adsorption of 10 important hazardous radionuclides—U, Th, Cs, Sr, Ce, Tc, La, Cr, Ni, Co—found in the nuclear fuel cycle.
Investigation by Digital Image Correlation of Mixed-Mode I and II Fracture Behavior of Polymeric IASCB Specimens with Additive Manufactured Crack-Like Notch
Authors: Brugo Tommaso Maria , Campione Ivo , Minak Giangiacomo
Published: February 2021

In this work, the fracture mechanics properties of polyamide (PA) specimens manufactured by the selective laser sintering (SLS) technology are investigated, in which an embedded crack-like notch was inserted in the design and produced during the additive manufacturing (AM) phase. To cover a wide variety of mode I/II mixity levels, the inclined asymmetrical semicircular specimen subjected to three points loading (IASCB) was employed. The investigation was carried out by analyzing the full displacement field in the proximity of the crack tip by means of the digital image correlation (DIC) technique. To characterize the material, which exhibits a marked elastic-plastic behavior, the quantity J-integral was evaluated by two different methods: the first one exploits the full fields measured by the DIC, whereas the second one exploits the experimental load–displacement curves along with FEM analysis. The DIC methodology was experimentally validated and proposed as an alternative method to evaluate the J-integral. It is especially suited for conditions in which it is not possible to use the conventional LDC method due to complex and possibly unknown loading conditions. Furthermore, results showed that the AM technique could be used effectively to induce cracks in this type of material. These two aspects together can lead to both a simplification of the fracture characterization process and to the possibility of dealing with a wider number of practical, real-world scenarios. Indeed, because of the nature of the additive manufacturing process, AM crack-like notches can be sintered even having complex geometry, being three-dimensional and/or inside the tested structure.
The Separation of Emulsified Water/Oil Mixtures through Adsorption on Plasma-Treated Polyethylene Powder
Authors: Abdulkareem Asma , Popelka Anton , Sobolčiak Patrik , Tanvir Aisha , Ouederni Mabrouk , AlMaadeed Mariam A. , Kasak Peter , Adham Samer , Krupa Igor
Published: February 2021

This paper addresses the preparation and characterization of efficient adsorbents for tertiary treatment (oil content below 100 ppm) of oil/water emulsions. Powdered low-density polyethylene (LDPE) was modified by radio-frequency plasma discharge and then used as a medium for the treatment of emulsified diesel oil/water mixtures in the concentration range from 75 ppm to 200 ppm. Plasma treatment significantly increased the wettability of the LDPE powder, which resulted in enhanced sorption capability of the oil component from emulsions in comparison to untreated powder. Emulsions formed from distilled water and commercial diesel oil (DO) with concentrations below 200 ppm were used as a model of oily polluted water. The emulsions were prepared using ultrasonication without surfactant. The droplet size was directly proportional to sonication time and ranged from 135 nm to 185 nm. A sonication time of 20 min was found to be sufficient to prepare stable emulsions with an average droplet size of approximately 150 nm. The sorption tests were realized in a batch system. The effect of contact time and initial oil concentrations were studied under standard atmospheric conditions at a stirring speed of 340 rpm with an adsorbent particle size of 500 microns. The efficiency of the plasma-treated LDPE powder in oil removal was found to be dependent on the initial oil concentration. It decreased from 96.7% to 79.5% as the initial oil concentration increased from 75 ppm to 200 ppm. The amount of adsorbed oil increased with increasing contact time. The fastest adsorption was observed during the first 30 min of treatment. The adsorption kinetics for emulsified oils onto sorbent followed a pseudo-second-order kinetic model.
Feasibility of Machine Learning Algorithms for Predicting the Deformation of Anodic Titanium Films by Modulating Anodization Processes
Authors: Kim Sung-Hee , Jeong Chanyoung
Published: February 2021

This study aims to demonstrate the feasibility of applying eight machine learning algorithms to predict the classification of the surface characteristics of titanium oxide (TiO2) nanostructures with different anodization processes. We produced a total of 100 samples, and we assessed changes in TiO2 nanostructures’ thicknesses by performing anodization. We successfully grew TiO2 films with different thicknesses by one-step anodization in ethylene glycol containing NH4F and H2O at applied voltage differences ranging from 10 V to 100 V at various anodization durations. We found that the thicknesses of TiO2 nanostructures are dependent on anodization voltages under time differences. Therefore, we tested the feasibility of applying machine learning algorithms to predict the deformation of TiO2. As the characteristics of TiO2 changed based on the different experimental conditions, we classified its surface pore structure into two categories and four groups. For the classification based on granularity, we assessed layer creation, roughness, pore creation, and pore height. We applied eight machine learning techniques to predict classification for binary and multiclass classification. For binary classification, random forest and gradient boosting algorithm had relatively high performance. However, all eight algorithms had scores higher than 0.93, which signifies high prediction on estimating the presence of pore. In contrast, decision tree and three ensemble methods had a relatively higher performance for multiclass classification, with an accuracy rate greater than 0.79. The weakest algorithm used was k-nearest neighbors for both binary and multiclass classifications. We believe that these results show that we can apply machine learning techniques to predict surface quality improvement, leading to smart manufacturing technology to better control color appearance, super-hydrophobicity, super-hydrophilicity or batter efficiency.
Environment-Friendly Preparation of Reaction-Bonded Silicon Carbide by Addition of Boron in the Silicon Melt
Authors: Rui Maoqiang , Zhang Yaxiang , Ye Jing
Published: February 2021

Reaction-bonded silicon carbide ceramics were sintered by infiltration of Si and B–Si alloy under an argon atmosphere at different temperatures. The element boron was added to the silicon melt to form a B–Si alloy first. The mechanical properties of samples were improved by infiltration of the B–Si melt. The samples infiltrated with the Si-only melt were found to be very sensitive to experimental temperature. The bending strengths of 58.6 and 317.0 MPa were achieved at 1530 and 1570 °C, respectively. The sample made by infiltration of B–Si alloy was successfully sintered at 1530 °C. The relative density of the sample was more than 90%. The infiltration of B–Si alloy reduced the sintering temperature and the bending strength reached 326.9 MPa. The infiltration mechanism of B–Si alloy is discussed herein.
Tuning Mg(OH)2 Structural, Physical, and Morphological Characteristics for Its Optimal Behavior in a Thermochemical Heat-Storage Application
Authors: Piperopoulos Elpida , Fazio Marianna , Mastronardo Emanuela , Lanza Maurizio , Milone Candida
Published: February 2021

Thermochemical materials (TCM) are among the most promising systems to store high energy density for long-term energy storage. To be eligible as candidates, the materials have to fit many criteria such as complete reversibility of the reaction and cycling stability, high availability of the material at low cost, environmentally friendliness, and non-toxicity. Among the most promising TCM, the Mg(OH)2/MgO system appears worthy of attention for its properties in line with those required. In the last few decades, research focused its attention on the optimization of attractive hydroxide performance to achieve a better thermochemical response, however, often negatively affecting its energy density per unit of volume and therefore compromising its applicability on an industrial scale. In this study, pure Mg(OH)2 was developed using different synthesis procedures. Reverse deposition precipitation and deposition precipitation methods were used to obtain the investigated samples. By adding a cationic surfactant (cetyl trimethylammonium bromide), deposition precipitation Mg(OH)2 (CTAB-DP-MH) or changing the precipitating precursor (N-DP-MH), the structural, physical and morphological characteristics were tuned, and the results were compared with a commercial Mg(OH)2 sample. We identified a correlation between the TCM properties and the thermochemical behavior. In such a context, it was demonstrated that both CTAB-DP-MH and N-DP-MH improved the thermochemical performances of the storage medium concerning conversion (64 wt.% and 74 wt.% respectively) and stored and released heat (887 and 1041 kJ/kgMg(OH)2). In particular, using the innovative technique not yet investigated for thermal energy storage (TES) materials, with NaOH as precipitating precursor, N-DP-MH reached the highest stored and released heat capacity per volume unit, ~684 MJ/m3.
Fabrication and Characterization of Viton@FOX-7@Al Spherical Composite with Improved Thermal Decomposition Property and Safety Performance
Authors: Li Xiaodong , Yang Yue , Song Changgui , Sun Yantao , Han Yuanqi , Zhao Yue , Wang Jingyu
Published: February 2021

To achieve a uniform distribution of the components and a better performance of aluminized composite explosives, Viton (dipolymers of hexafluoropropylene and vinylidene fluoride) @ FOX-7 (1,1-diamino-2,2-dinitroethylene) @Al microspheres and FOX-7/Viton@Al were synthesized by spray-drying strategy contrastively. Viton@FOX-7@Al owned porous and loose morphology and good sphericity with a retained crystal phase of FOX-7 and aluminum. The 23.56% fluorine content on Viton@FOX-7@Al surface indicated that Viton was completely coated on the surface of the particles. Nanosized aluminum (nAl) in Viton@FOX-7@Al had a certain catalytic activity on the thermal decomposition process of FOX-7 resulting in a depressed exothermic peak temperature and reduced apparent activation energy relative to nAl in FOX-7/Viton@Al. Because of the specific structure and the synergies between each individual component, Viton@FOX-7@Al showed reduced impact sensitivity and friction sensitivity than those of FOX-7/Viton@Al. In brief, Viton@FOX-7@Al with multilevel coating structure possessed comparatively low thermal decomposition energy requirement and improved safety performance.
Alloplastic Bone Substitutes for Periodontal and Bone Regeneration in Dentistry: Current Status and Prospects
Authors: Fukuba Shunsuke , Okada Munehiro , Nohara Kohei , Iwata Takanori
Published: February 2021

Various bone graft products are commercially available worldwide. However, there is no clear consensus regarding the appropriate bone graft products in different clinical situations. This review is intended to summarize bone graft products, especially alloplastic bone substitutes that are available in multiple countries. It also provides dental clinicians with detailed and accurate information concerning these products. Furthermore, it discusses the prospects of alloplastic bone substitutes based on an analysis of the current market status, as well as a comparison of trends among countries. In this review, we focus on alloplastic bone substitutes approved in the United States, Japan, and Korea for use in periodontal and bone regeneration. According to the Food and Drug Administration database, 87 alloplastic bone graft products have been approved in the United States since 1996. According to the Pharmaceuticals and Medical Devices Agency database, 10 alloplastic bone graft products have been approved in Japan since 2004. According to the Ministry of Health and Welfare database, 36 alloplastic bone graft products have been approved in Korea since 1980. The approved products are mainly hydroxyapatite, β-tricalcium phosphate, and biphasic calcium phosphate. The formulations of the products differed among countries. The development of new alloplastic bone products has been remarkable. In the near future, alloplastic bone substitutes with safety and standardized quality may be the first choice instead of autologous bone; they may offer new osteoconductive and osteoinductive products with easier handling form and an adequate resorption rate, which can be used with growth factors and/or cell transplantation. Careful selection of alloplastic bone graft products is necessary to achieve predictable outcomes according to each clinical situation.
Echinoderms Metabolites: Structure, Functions, and Biomedical Perspectives
Authors: Kalinin Vladimir I.
Published: February 2021

Echinoderms are marine invertebrates belonging to the phylum Echinodermata (from the Ancient Greek words “echinos” (hedgehog) and “derma” (skin)) [...]
Effectiveness of Smartwatch Guidance for High-Quality Infant Cardiopulmonary Resuscitation: A Simulation Study
Authors: Jeon Seong A , Chang Hansol , Yoon Sun Young , Hwang Nayeong , Kim Kyunga , Yoon Hee , Hwang Sung Yeon , Shin Tae Gun , Cha Won Chul , Kim Taerim
Published: February 2021

Background and objectives: As in adults, the survival rates and neurological outcomes after infant Cardiopulmonary resuscitation (CPR) are closely related to the quality of resuscitation. This study aimed to demonstrate that using a smartwatch as a haptic feedback device increases the quality of infant CPR performed by medical professionals. Materials and methods: We designed a prospective, randomized, case-crossover simulation study. The participants (n = 36) were randomly allocated to two groups: control first group and smartwatch first group. Each CPR session consisted of 2 min of chest compressions (CCs) using the two-finger technique (TFT), 2 min of rest, and 2 min of CCs using the two-thumb encircling hands technique (TTHT). Results: The primary outcome was the variation in the “proportion of optimal chest compression duration” and “compression rate” between the smartwatch-assisted and non-smartwatch-assisted groups. The secondary outcome was the variation in the “compression depth” between two groups. The proportion of optimal CC duration was significantly higher in the smartwatch-assisted group than in the non-smartwatch-assisted group. The absolute difference from 220 was much smaller in the smartwatch-assisted group (218.02) than in the non-smartwatch-assisted group (226.59) (p-Value = 0.018). Conclusion: This study demonstrated the haptic feedback system using a smartwatch improves the quality of infant CPR by maintaining proper speed and depth regardless of the compression method used.
Our Evolving Understanding of ME/CFS
Authors: Friedman Kenneth J. , Murovska Modra , Pheby Derek F. H. , Zalewski Paweł
Published: February 2021

The potential benefits of the scientific insights gleaned from years of treating ME/CFS for the emerging symptoms of COVID-19, and in particular Longhaul- or Longhauler-COVID-19 are discussed in this opinion article. Longhaul COVID-19 is the current name being given to the long-term sequelae (symptoms lasting beyond 6 weeks) of SARS-CoV-2 infection. Multiple case definitions for ME/CFS exist, but post-exertional malaise (PEM) is currently emerging as the ‘hallmark’ symptom. The inability to identify a unique trigger of ME/CFS, as well as the inability to identify a specific, diagnostic laboratory test, led many physicians to conclude that the illness was psychosomatic or non-existent. However, recent research in the US and the UK, championed by patient organizations and their use of the internet and social media, suggest underlying pathophysiologies, e.g., oxidative stress and mitochondrial dysfunction. The similarity and overlap of ME/CFS and Longhaul COVID-19 symptoms suggest to us similar pathological processes. We put forward a unifying hypothesis that explains the precipitating events such as viral triggers and other documented exposures: For their overlap in symptoms, ME/CFS and Longhaul COVID-19 should be described as Post Active Phase of Infection Syndromes (PAPIS). We further propose that the underlying biochemical pathways and pathophysiological processes of similar symptoms are similar regardless of the initiating trigger. Exploration of the biochemical pathways and pathophysiological processes should yield effective therapies for these conditions and others that may exhibit these symptoms. ME/CFS patients have suffered far too long. Longhaul COVD-19 patients should not be subject to a similar fate. We caution that failure to meet the now combined challenges of ME/CFS and Longhaul COVID-19 will impose serious socioeconomic as well as clinical consequences for patients, the families of patients, and society as a whole.
A Typical Case Presentation with Spontaneous Visual Recovery in Patient Diagnosed with Leber Hereditary Optic Neuropathy Due to Rare Point Mutation in MT-ND4 Gene (m.11253T>C) and Literature Review
Authors: Liutkeviciene Rasa , Sidaraite Agne , Kuliaviene Lina , Glebauskiene Brigita , Jurkute Neringa , Aluzaite-Baranauskiene Lina , Gelzinis Arvydas , Zemaitiene Reda
Published: February 2021

Leber hereditary optic neuropathy (LHON) is one of the most common inherited mitochondrial optic neuropathies, caused by mitochondrial DNA (mtDNA) mutations. Three most common mutations, namely m.11778G>A, m.14484T>G and m.3460G>A, account for the majority of LHON cases. These mutations lead to mitochondrial respiratory chain complex I damage. Typically, LHON presents at the 15–35 years of age with male predominance. LHON is associated with severe, subacute, painless bilateral vision loss and account for one of the most common causes of legal blindness in young individuals. Spontaneous visual acuity recovery is rare and has been reported in patients harbouring m.14484T>C mutation. Up to date LHON treatment is limited. Idebenone has been approved by European Medicines Agency (EMA) to treat LHON. However better understanding of disease mechanisms and ongoing treatment trials are promising and brings hope for patients. In this article we report on a patient diagnosed with LHON harbouring rare m.11253T>C mutation in MT-ND4 gene, who experienced spontaneous visual recovery. In addition, we summarise clinical presentation, diagnostic features, and treatment.
Does Surgical Margin Width Remain a Challenge for Triple-Negative Breast Cancer? A Retrospective Analysis
Authors: Bonci Eduard-Alexandru , Țîțu Ștefan , Petrușan Alexandru Marius , Hossu Claudiu , Gâta Vlad Alexandru , Ghomi Morvarid Talaeian , Kubelac Paul Milan , Bonci Teodora Irina , Piciu Andra , Cosnarovici Maria , Hîțu Liviu , Kirsch-Mangu Alexandra Timea , Pop Diana Cristina , Lisencu Ioan Cosmin , Achimaș-Cadariu Patriciu , Piciu Doina , Schmidt Hank , Fetica Bogdan
Published: February 2021

Background and Objectives: Local and distant relapse (LR, DR) in breast cancer vary according to its molecular subtypes, with triple-negative breast cancer (TNBC) being the most aggressive. The surgical resection margin width (SRMW) for breast-conserving surgery (BCS) has been intensely debated, especially for the aforementioned subtype. The aim of this study was to examine the impact of SRMW on LR following BCS in TNBC patients. Materials and Methods: We conducted a retrospective study including all patients with TNBC for whom BCS was performed between 2005 and 2014. Results: Final analysis included a total of 92 patients, with a median tumor size of 2.5 cm (range 0–5 cm) and no distant metastasis at the time of diagnosis. A total of 87 patients had received neoadjuvant and/or adjuvant chemotherapy, and all patients had received adjuvant whole-breast radiotherapy. After a median follow-up of 110.7 months (95% CI, 95.23–126.166), there were 5 local recurrences and 8 regional/distant recurrences with an overall LR rate of 5.4%. The risk of LR and DR was similar between groups of patients with several SRMW cut-off values. Conclusions: Our study supports a safe “no ink on tumor” approach for TNBC patients treated with BCS.
Relationship between Trace Element in Tumor and Prognosis in Lung Cancer Patients
Authors: Saikawa Hirotaka , Nagashima Hiromi , Cho Katsuya , Chiba Ryosuke , Sera Koichiro , Shigeeda Wataru , Tomoyasu Makoto , Deguchi Hiroyuki , Takahashi Fumiaki , Saito Hajime , Sugai Tamotsu , Maemondo Makoto
Published: February 2021

Background and Objectives: This study aimed to observe the relationship between trace element concentrations in lung tissue from lung non-small cell lung carcinoma (NSCLC) patients and prognosis. Materials and Methods: The concentrations of various trace elements in the lung tissues were measured by a particle-induced X-ray emission (PIXE) system, and the results were analyzed for statistical significance. Eight essential trace elements, Cr, Mn, Fe, Co, Cu, Zn, Se, and Mo, were analyzed. We investigated the relationship between trace element concentrations and disease-free survival (DFS) and overall survival (OS) in NSCLC patients. Results: A total of 129 NSCLC patients and 20 control patients were included in this study. As for DFS, Co was the only element that showed a significant difference, and the high Co group had better DFS (HR: 0.352, 95% CI = 0.128–0.97). No significant difference was observed for Cr, Mn, Fe, Se, or Mo, but DFS tended to be better in the high trace element group. No significant difference was observed for Cu and Zn, but DFS tended to be good in the low trace element group. As for OS, Cr was the only element that showed a significant difference, and the high Cr element group had better OS (HR: 0.477, 95% CI = 0.128–0.97). Conclusions: This study suggests that the prognosis is good in lung cancer cases with high intratumoral concentrations of Co and Cr. The dynamics of trace elements in body and in tumor tissue have not been well established, and we consider that more research is necessary in the future.
Branched High Aspect Ratio Nanostructures Fabricated by Focused Helium Ion Beam Induced Deposition of an Insulator
Authors: Allen Frances I.
Published: February 2021

Helium ion beam induced deposition using the gaseous precursor pentamethylcyclopentasiloxane is employed to fabricate high aspect ratio insulator nanostructures (nanopillars and nanocylinders) that exhibit charge induced branching. The branched nanostructures are analyzed by transmission electron microscopy. It is found that the side branches form above a certain threshold height and that by increasing the flow rate of the precursor, the vertical growth rate and branching phenomenon can be significantly enhanced, with fractalesque branching patterns observed. The direct-write ion beam nanofabrication technique described herein offers a fast single-step method for the growth of high aspect ratio branched nanostructures with site-selective placement on the nanometer scale.
An Ultra-High-Q Lithium Niobate Microresonator Integrated with a Silicon Nitride Waveguide in the Vertical Configuration for Evanescent Light Coupling
Authors: Zhang Jianhao , Wu Rongbo , Wang Min , Liang Youting , Zhou Junxia , Wu Miao , Fang Zhiwei , Chu Wei , Cheng Ya
Published: February 2021

We demonstrate the hybrid integration of a lithium niobate microring resonator with a silicon nitride waveguide in the vertical configuration to achieve efficient light coupling. The microring resonator is fabricated on a lithium niobate on insulator (LNOI) substrate using photolithography assisted chemo-mechanical etching (PLACE). A fused silica cladding layer is deposited on the LNOI ring resonator. The silicon nitride waveguide is further produced on the fused silica cladding layer by first fabricating a trench in the fused silica while using focused ion beam (FIB) etching for facilitating the evanescent coupling, followed by the formation of the silicon nitride waveguide on the bottom of the trench. The FIB etching ensures the required high positioning accuracy between the waveguide and ring resonator. We achieve Q-factors as high as 1.4 × 107 with the vertically integrated device.
EGCG, a Green Tea Catechin, as a Potential Therapeutic Agent for Symptomatic and Asymptomatic SARS-CoV-2 Infection
Authors: Chourasia Mukesh , Koppula Purushotham , Battu Aruna , Ouseph Madhu , Singh Anil
Published: February 2021

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has emerged to be the greatest threat to humanity in the modern world and has claimed nearly 2.2 million lives worldwide. The United States alone accounts for more than one fourth of 100 million COVID-19 cases across the globe. Although vaccination against SARS-CoV-2 has begun, its efficacy in preventing a new or repeat COVID-19 infection in immunized individuals is yet to be determined. Calls for repurposing of existing, approved, drugs that target the inflammatory condition in COVID-19 are growing. Our initial gene ontology analysis predicts a similarity between SARS-CoV-2 induced inflammatory and immune dysregulation and the pathophysiology of rheumatoid arthritis. Interestingly, many of the drugs related to rheumatoid arthritis have been found to be lifesaving and contribute to lower COVID-19 morbidity. We also performed in silico investigation of binding of epigallocatechin gallate (EGCG), a well-known catechin, and other catechins on viral proteins and identified papain-like protease protein (PLPro) as a binding partner. Catechins bind to the S1 ubiquitin-binding site of PLPro, which might inhibit its protease function and abrogate SARS-CoV-2 inhibitory function on ubiquitin proteasome system and interferon stimulated gene system. In the realms of addressing inflammation and how to effectively target SARS-CoV-2 mediated respiratory distress syndrome, we review in this article the available knowledge on the strategic placement of EGCG in curbing inflammatory signals and how it may serve as a broad spectrum therapeutic in asymptomatic and symptomatic COVID-19 patients.
Optimization and Anti-Cancer Properties of Fluoromethylketones as Covalent Inhibitors for Ubiquitin C-Terminal Hydrolase L1
Authors: Krabill Aaron D. , Chen Hao , Hussain Sajjad , Hewitt Chad S. , Imhoff Ryan D. , Muli Christine S. , Das Chittaranjan , Galardy Paul J. , Wendt Michael K. , Flaherty Daniel P.
Published: February 2021

The deubiquitinating enzyme (DUB) UCHL1 is implicated in various disease states including neurodegenerative disease and cancer. However, there is a lack of quality probe molecules to gain a better understanding on UCHL1 biology. To this end a study was carried out to fully characterize and optimize the irreversible covalent UCHL1 inhibitor VAEFMK. Structure-activity relationship studies identified modifications to improve activity versus the target and a full cellular characterization was carried out for the first time with this scaffold. The studies produced a new inhibitor, 34, with an IC50 value of 7.7 µM against UCHL1 and no observable activity versus the closest related DUB UCHL3. The molecule was also capable of selectively inhibiting UCHL1 in cells and did not demonstrate any discernible off-target toxicity. Finally, the molecule was used for initial probe studies to assess the role of UCHL1 role in proliferation of myeloma cells and migration behavior in small cell lung cancer cells making 34 a new tool to be used in the biological evaluation of UCHL1.