bluuebunny/crossref_metadata_2025 huggingface dataset:
Time matters: chrono-pharmacotherapy as precision medicine
Authors: Arabi Samir , Ahmad Sajjad
Published: February 2025

The circadian clock and its inherent rhythms serve as the foundation for regulating many of the body’s vital processes. Recent advances in medicine have leveraged these natural cycles through chronotherapy, the practice of timing drug administration to align with the body's internal clock, to enhance treatment outcomes. Endogenous clocks not only dictate physiological rhythms but also influence how drugs are absorbed, distributed, metabolized, and excreted, thereby affecting both their therapeutic efficacy and potential toxicity. When these circadian rhythms are disrupted, the resulting imbalance has been linked to a range of disorders, such as cancer and cardiovascular diseases, highlighting the growing need for time-sensitive therapeutic strategies. Technological breakthroughs, including wearable sensors, digital health platforms, and sophisticated machine learning techniques, now allow for continuous, real-time monitoring of circadian biomarkers, which in turn helps optimize treatment regimens. In oncology, for example, synchronizing chemotherapy, cardiovascular therapy, immunotherapy and anti-inflammatory drugs with the patient’s circadian cycle has been shown to enhance drug effectiveness and improve tolerability. Moreover, observed sex-based differences in circadian drug responses emphasize the importance of tailoring drugs to individual patient profiles. Emerging research is also focusing on directly targeting molecular clock components, showing potential for treating metabolic and oncological conditions. However, fully integrating chronopharmacology into clinical practice will require the development of standardized protocols, regulatory backing, and large-scale trials that incorporate circadian biomarkers. Ultimately, merging circadian biology with therapeutic strategies promises to optimize treatment efficacy, minimize side effects, and enhance patient outcomes. Future studies must address inter-individual variability, sex differences, and circadian misalignment to fully harness chronotherapy’s potential in personalized medicine.
Reseña de Bartra (2022) Exceso de muerte: De la peste de Atenas a la covid 19
Authors: Gonzalez Molohua Yolanda Francisca
Published: February 2025

Armando Bartra, habla en esta obra de un panorama en el que imperan las crisis;  económica, social, ambiental y de salud,  ante  las pandemias y los virus que como consecuencia potencian otros males sociales como la pobreza, la desigualdad social,  la exclusión, y la vulnerabilidad  ante enfermedad y la muerte. El autor da cuenta de un ciclo de crisis  que se retroalimenta entre sí,  y que han trastocado el orden social  en múltiples aspectos que tienen que ver con el sistema de salud, la educación y lo laboral, se tratan de crisis estructurales que históricamente han contribuido a aumentar los grados de desigualdad social.
Reseña de Stern (2022) El cuenco vacío: Aportaciones de un psicoanalista al estudio del buddhadhrma
Authors: Palacios Montoya José Carlos
Published: February 2025

Reseña de Stern, Sergio (2022). El cuenco vacío: Aportaciones de un psicoanalista al estudio del buddhadhrma. Gedisa.
Bosquejo de la niñez mexicana, a partir del primer Boletín de la SEP
Authors: Terán Fuentes Aurora
Published: February 2025

El inicio de la etapa de reconstrucción nacional dentro del proceso de la Revolución Mexicana se dio con el gobierno de Álvaro Obregón (1920-1924). La educación fue prioritaria en la definición de políticas. Para tal propósito, se creó la Secretaría de Educación Pública (SEP) en 1921, con el fin de impulsar proyectos educativos a nivel federal con alcance nacional. El primer titular de la SEP fue José Vasconcelos. Ante un panorama complejo centrado en la niñez, definido a partir de la visión oficial de la nueva Secretaría, se implementaron acciones para combatir, desde la escuela pública, problemas como el analfabetismo, la desnutrición, la falta de higiene y las enfermedades; expuestos en el primer Boletín de la Secretaría de Educación Pública. Con base en el boletín citado, se construye la concepción de la niñez mexicana, permeada por los problemas mencionados, recuperando dos referentes: la imagen occidental de la infancia y la definición de los derechos de los niños.
Susceptibilidad y amenaza asociadas a la subsidencia y colapsos en terrenos kársticos: Caso del estado de Campeche
Authors: Palacio-Aponte Álvaro Gerardo
Published: February 2025

Este trabajo evalúa la susceptibilidad a la subsidencia y colapsos en terrenos kársticos de Campeche, México, asumiendo que bajo un conjunto de factores condicionantes estos fenómenos ocurren con mayor o menor intensidad y pueden representar una amenaza para la población y las actividades económicas. El modelo conceptual propone la ponderación relativa de zonas susceptibles mediante análisis multicriterio de jerarquías analíticas incluyendo factores litológicos, estructurales y ambientales que inciden en la geomecánica de la subsidencia y colapsos. El modelo conceptual se traduce a uno lógico en un Sistema de Información Geográfica (ArcMap) a partir del cual se obtiene un mapa de susceptibilidad con cuatro niveles, muy alta con el 13% de superficie estatal, alta 25%, media 30% y 32% baja. Los valores de susceptibilidad corresponden con los niveles de predisposición del terreno a la amenaza. Se concluye que el mapa de susceptibilidad es un documento cartográfico preventivo de amenaza...
Detección, modelización y proyección del crecimiento urbano de núcleos de población en diferentes cuencas hidrológicas de Chihuahua, México
Authors: García-Ramírez Pedro , Alatorre-Cejudo Luis Carlos , Bravo-Peña Luis Carlos
Published: February 2025

El objetivo fue detectar, modelar y proyectar los crecimientos urbanos de núcleos de población en diferentes cuencas hidrológicas de Chihuahua, México. Se modelaron los crecimientos urbanos para predecir escenarios futuros 2030, 2040 y 2050 utilizando regresión logística (RL) y CA-Markov incorporado en Modelador de Cambio en el Terreno (LCM) integrado en IDRISI. Se produjeron mapas de uso de suelo años 2010, 2015 y 2020 utilizando Máquina de Soporte Vectorial (SVM). Los cambios de uso de suelo (LULC) de mayor intensidad se presentaron en el período 2010-2015, el incremento de las áreas urbanas proviene en su mayoría de los bosques. El modelo de RL resultó ser consistente mostrando Pseudo R2 de McFadden superior a 0.2. Los Valores de Característica Operativa del Receptor (ROC), se consideran aceptables. En el uso de suelo urbano simulado se observa mayor crecimiento en el año 2030, para el 2040 y 2050 se identifica una tasa baja.
maestro de biología y su conocimiento profesional para la enseñanza. Una mirada de tres modelos latinoamericanos
Authors: Medina-Mendoza Indira Viridiana , Alfonso-Páez David , Gómez-Galindo Alma Adrianna
Published: February 2025

Una dificultad que atañe a la enseñanza de la biología en Latinoamérica es la calidad de la formación del profesorado, específicamente, se cuestiona el conocimiento que debe poseer el docente. Entonces, ¿qué modelos teóricos o metodológicos existen en Latinoamérica sobre el conocimiento del profesor de biología y qué ofrecen a la formación docente? El objetivo del artículo es hacer una reflexión teórica sobre tres modelos latinoamericanos del conocimiento profesional del profesor de biología. Se pretende aportar en el entendimiento de modelos útiles en la formación inicial y continua de los maestros de biología, así como brindar una revisión teórica de los modelos de conocimiento profesional elaborados desde Latinoamérica.  Estos modelos dan cuenta de la influencia cultural y biográfica, así como el contexto real, por ello son adecuados para la formación y mejora de la práctica del maestro latinoamericano.
Marcadores de inflamación, riesgo cardiovascular y consumo dietético en pacientes con síndrome metabólico de la Laguna de Durango
Authors: Medina-Ayala Dayna Lizett , Villarreal-Martínez Ángel Eduardo , Maravilla-Domínguez María Aurora , Martínez-Sandoval Alondra , Guangorena-Gómez Janeth Oliva , Muñoz-Yáñez Claudia , Zavaleta-Muñiz Soraya Amali
Published: February 2025

El síndrome metabólico (SM) es conjunto de enfermedades crónicas caracterizado por cambios bioquímicos e inflamatorios con riesgo aumentado de morbimortalidad. El objetivo fue describir los marcadores de inflamación, el riesgo cardiovascular y el consumo dietético en sujetos con SM. Se incluyeron para análisis transversal sujetos con SM de dos Unidades Médicas de Atención Especializada en Enfermedades Crónicas (UNEME-EC) de la región Laguna del estado de Durango. El 100% de los sujetos presentaron obesidad abdominal, 34.4% exhibió un riesgo cardiovascular intermedio-alto, niveles de IPL e INL por encima de la media en el 49% y 45.2% respectivamente. El consumo dietético fue elevado en calorías, cereales y alimentos de origen animal y bajo en fibra.  El riesgo elevado de morbimortalidad a 10 años encontrado en estos sujetos requiere un mayor control y la evaluación de factores asociados al descontrol metabólico e inflamatorio que presentan.
Hidroxiapatita y selenio: Protagonistas emergentes en la lucha contra el cáncer
Authors: López-Ortiz Susana , Salado-Leza Daniela
Published: February 2025

La hidroxiapatita sintética (sHap) es un material biomimético con propiedades remarcables; es biocompatible y biodegradable, por lo que es asimilado por el cuerpo humano. Además de sus conocidas aplicaciones en ingeniería de tejidos, la sHap han mostrado ser una candidata prometedora para combatir el cáncer, actuando principalmente como una matriz para otros agentes terapéuticos. Presenta una estructura química con gran potencial para sustitución iónica con el objetivo de incrementa sus características y propiedades de manera sostenida, prolongada y dirigida contra el cáncer. La incorporación de elementos específicos, como el selenio (Se), coadyuva en la inhibición del crecimiento y propagación de células cancerosas, además de favorecer una respuesta inmunitaria, y prevención. Este documento es una revisión actual de los métodos de obtención de la sHap y su dopaje con Se. Discutimos aspectos de biocompatibilidad y citotoxicidad, además de su efecto documentado tanto en células sanas como cancerosas.
Cuando Álvarez Bravo se convirtió en amateur. Lectura fotográfica y contextos comunicativos
Authors: González-Ponce Citlalli
Published: February 2025

El artículo presenta un ejercicio experimental de “lectura fotográfica” que indaga sobre los contextos de la imagen. Siguiendo la idea de la contundencia de la fotografía se muestra una serie de imágenes contenidas en el libro Oaxacagrafía. Este libro presenta once fotografías capturadas en diferentes lugares de la ciudad de Oaxaca, entre 1962 y 2018, por once fotógrafos clásicos y contemporáneos. Los resultados del ejercicio muestran que el conocimiento previo de los nombres de autores, de sus vidas o de las corrientes artísticas a las que pertenecen, aporta más a la lectura realizada que la propia imagen que observan.
Formulasi Sediaan Gel Face Scrub Dari Ekstrak Etanol Bayam Merah (Amaranthus tricolor L)
Authors: Rosi Devahimer Harsep
Published: February 2025

Telah dilakukan penelitian “Formulasi Sediaan Gel Face Scrub Dari Ekstrak Etanol Bayam Merah (Amaranthus tricolor L).” Tujuan dari penelitian ini untuk mengetahui ekstrak etanol bayam merah dapat diformulasikan sebagai sediaan gel face scrub. Ekstraksi sampel dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak yang didapatkan dibuat gel face scrub. Pada penelitian ini dibuat menjadi 3 formulasi sediaan gel face scrub dengan konsentrasi yang berbeda yaitu F1 0,5 g, F2 0,75 g, dan F3 1 g ekstrak. Dilakukan evaluasi sediaan gel face scrub yaitu meliputi uji organoleptis, uji homogenitas, uji pH, uji iritasi, uji daya sebar dan uji daya lekat. Bayam merah yang digunakan telah diidentifikasi sebagai Amaranthus tricolor L. Hasil skrining fitokimia terhadap ekstrak bayam merah menunjukkan ekstrak positif mengandung flavonoid, alkaloid, steroid, saponin, dan tanin. Kesimpulan yang didapat bahwa sediaan gel face scrub dari ekstrak etanol bayam merah dapat diformulasikan menjadi sediaan gel face scrub karena sediaan gel yang diperoleh telah memenuhi parameter uji fisik yaitu uji organoleptis, uji homogenitas, uji pH, uji iritasi kulit, uji daya lekat, namun belum memenuhi persyaratan terhadap uji daya sebar.
Nunca es tarde para aprender: importancia de la alfabetización digital en adultos mayores
Authors: Molano Monsalve Gabriela Sofia , Rincón Guatibonza David Orlando , Martínez Montaño Juan Camilo , Ramírez Rodriguez Fabian Alberto
Published: February 2025

Actualmente el desarrollo de las tecnologías crece rápidamente y es por esto que los adultos mayores se ven obligados a aprender deprisa para no quedar relegado, produciendo brechas en cuanto a la información y maneras de acceder a ella entre las diferentes generaciones, sobre todo en los adultos mayores. Se ha realizado esta investigación con el fin de analizar la importancia del uso de dispositivos móviles en adultos mayores, como impulsor de habilidades nuevas en su desarrollo personal. La metodología para seguir fue por medio de un estudio cuantitativo de carácter descriptivo a dos poblaciones diferentes mediante encuestas en formularios de Google, la muestra fue dividida en dos partes, la primera consta de 23 adultos mayores entre 60 y 92 años y 20 profesionales de la salud entre los 25 y 56 años. Se realizaron análisis de condición social y de salud mental y aptitudes en relación con el manejo de dispositivos móviles, constatando que los adultos mayores mostraron un gran interés en el manejo de dispositivos móviles y considerando importante el uso de estos en la actualidad, así mismo un gran número usaba su celular de manera básica. En la segunda parte, casi todos los trabajadores de la salud manifestaron la importancia del aprendizaje tomando en cuenta los intereses y necesidades del adulto mayor, finalmente más de la mitad apoyó el uso de dispositivos móviles como herramienta pedagógica, dejando claro que es indispensable empezar un proceso de alfabetización digital en adultos mayores.
Klasifikasi Gangguan Tidur Menggunakan Metode Decision Tree dan Algoritma Genetika
Authors: Septiani Wisti Dwi
Published: February 2025

Pola tidur yang baik berperan penting dalam menjaga kesehaatan tubuh serta keseimbangan fisik dan mental seseorang. Gaya hidup yang sehat seperti pola makan, aktivitas fisik dan kebiasaan pola hidup sehat berperan penting dalam mempengaruhi kualitas tidur. Gangguan tidur merupakan masalah kesehatan yang semakin banyak dialami oleh individu global saat ini dan berpotensi memberikan dampak negatif terhadap kualitas hidup mereka. Dalam era kecerdasan buatan, analisis data menjadi salah satu pendekatan yang efektif untuk memahami pola dan prediksi terkait kesehatan. Tujuan penelitian ini adalah memprediksi gangguan tidur berdasarkan kualitas tidur dan gaya hidup. menggunakan algoritma klasifikasi data mining. Metode yang digunakan dalam penelitian ini adalah implementasi metode Decision Tree, dengan seleksi fitur menggunakan Algoritma Genetika untuk meningkatkan akurasi metode. Hasil penelitian ini adalah kenaikan nilai akurasi sebesar 10,72% dengan pengujian optimasi menggunakan metode Algoritma Genetika, juga dihasilkan pohon keputusan dari metode Decision Tree. Kesimpulan dari penelitian ini adalah metode optimasi Algoritma Genetika dapat membantu meningkatkan kehandalan dan rule yang dihasilkan dari pohon keputusan Metode Decision Tree dipilih karena mampu memberikan prediksi yang akurat dan menghasilkan rule dari phon keputusan yang mudah dipahami dan dapat membantu kita untuk memprediksi gangguan tidur sehingga dapat meningkatkan kesadaran akan pentingnya hidup sehat dengan menjaga kualitas tidur dan gaya hidup yang sehat. Hasil penelitian ini juga menunjukkan bahwa durasi tidur, tingkat stres, aktivits fisik, umur, dan BMI memberikan pengaruh signifikan terhadap kualitas tidur sesesorang.
Inclusion of queer curricula in language education: A transformative teaching and learning framework
Authors: Uys Matthys , Romylos Salomé , Nel Carisma
Published: February 2025

Research has shown that South Africa is a queerphobic country where heterosexual citizens either reject or merely tolerate queer individuals whose gender identity or sexual orientation differs from the heteronormative standard. Against this background, the present study argues that a queer-inclusive transformative teaching and learning framework (i.e., content, pedagogical, technological and pedagogical content knowledge) for the language classroom can be used to address queer social injustices and create awareness and understanding of gender identities and sexual orientations. A qualitative case study design within a transformative paradigm was employed. The methods included purposive sampling and questionnaires for data generation, and content analysis as a data analysis strategy. Responses to these questionnaires elucidated the perceptions of preservice teachers and language lecturers regarding the teaching of queer texts and how prepared they felt to do so. Factors hindering teachers’ willingness to teach queer texts are unpreparedness and limitations about personal values and beliefs. The framework suggested is based on Mezirow’s “perspective transformation” to instil a mind change among in-service teachers and language lecturers regarding the teaching and learning of queer curricula to resist and combat queerphobia and contribute to a more inclusive environment for queer individuals. The framework also provides an outline of a queer curriculum with the incorporation of the backward design to lesson planning.
Prevention of Neurological Damage in Severe Cervical Stenosis: The Role of Intraoperative Monitoring and Proactive Measures
Authors:   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Cembraneli Pedro Nogarotto , Cavalcante Julia Brasileiro de Faria ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Cembraneli Italo Nogarotto ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Cavalcante Renata Brasileiro de Faria ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Cavalcante José Edison da Silva ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Lopes Leonardo Taveira ,   Department of Radiology, Hospital of Neurology Santa Mônica, Goiânia, GO, Brazil, Cardoso Alessandro Fonseca ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Xavier Marco Daniel ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil, Cardoso Chrystiano Fonseca ,   ¹Department of Neurosurgery, Hospital of Neurology Santa Mônica, Goiânia,GO, Brazil
Published: February 2025

Cervical stenosis is a degenerative condition that causes compression of the spinal cord and nerve roots,
resulting in neurological symptoms such as pain, weakness, and loss of motor and sensory function.
Surgical treatment, such as spinal decompression, is indicated for severe cases, as untreated compression
may lead to irreversible damage. Intraoperative neurophysiological monitoring (IONM) has proven
essential for patient safety, allowing early detection of neurological changes and enabling immediate
intervention. This article discusses the importance of IONM in severe cervical stenosis surgeries,
highlighting preventive measures to minimize neurological damage. We report the case of a 57-yearold patient with severe cervical stenosis associated with myelopathy. A posterior surgical approach
was chosen, with decompression and fusion, utilizing IONM to monitor motor potentials. Proactive
measures, such as warm saline irrigation and maintenance of a mean arterial pressure (MAP) of 90
mmHg, were implemented to prevent neurological deterioration. After decompression, motor potentials
returned to baseline levels, and the patient recovered without new neurological deficits. We conclude
that IONM, combined with preventive strategies, is crucial in reducing the risk of neurological damage
during severe cervical stenosis surgeries, ensuring greater patient safety
Oropouche: Revisão integrativa acerca das implicações da transmissão vertical em um hospedeiro humano
Authors: Chaves Hellyângela Maria da Silva , Silva Kayllane Kelssiney da , Santos Maria Renata da Silva , Gomes Ana Lisa do Vale
Published: February 2025

O vírus Oropouche é um arbovírus emergente, mas não recente, que na maioria dos casos provoca sintomas leves, semelhantes aos da dengue, como dor de cabeça, dor muscular, náuseas e erupções cutâneas. Contudo, foi relatado, em 2024 no Brasil, casos de febre de Oropouche no qual houve transmissão do vírus durante a gravidez causando uma morte fetal e um aborto espontâneo no estado de Pernambuco. Ressalta-se que não apenas a morte fetal pode estar associada a essa infecção, como também potenciais efeitos neurológicos podem ser causados, sendo essas complicações análogas à infecção do Zika vírus. Diante disso, objetivou-se com essa revisão integrativa realizar uma busca sobre as informações acerca da transmissão vertical em casos de infecção pelo vírus Oropouche e sintetizar neste artigo os dados, ainda em investigação, relativos ao Brasil. Nessa perspectiva, o material utilizado para desenvolver essa pesquisa foi obtido nas bases de dados PubMed e BVS, a partir dos descritores “Vírus Oropouche” e “Transmissão vertical”, para artigos em inglês ou português entre os anos de 2015 e 2025. Nessa revisão, observou-se que nenhum material aborda especificamente sobre as implicações da transmissão vertical do vírus Oropouche durante a gravidez, evidenciando assim a necessidade de mais estudos com esta temática, visto que a ocorrência desses casos no país indica um alerta em saúde. Assim, é possível verificar a importância do estudo da Virologia, uma vez que por meio dela será possível analisar as consequências dessa doença, bem como prevenir uma possível epidemia como a vivenciada pela Zika.
Testemunhas de Jeová e Hemotransfusão: Desafios e implicações éticas na atualidade
Authors: Bastos Melyssa Versiani , Altíssimo Ana Laura Souza do , Lima Andiara Gomes de , Neiva Carolina Abreu , Fonseca Giovana Nahass Querubino Guimarães , Stahlberg Luana Lagares , Oliveira Raquel Motta de , Leal Sofia Barbosa , Villalobos Maria Isabel de Oliveira e Britto
Published: February 2025

Introdução: Questões éticas e bioéticas são abordadas quando se coloca em discussão a recusa dos Testemunhas de Jeová (TJ) em aceitar transfusões sanguíneas. O debate envolve princípios fundamentais: a autonomia e a beneficência. O artigo objetivou avaliar os pontos positivos e negativos relacionados à tomada de decisão médica em transfundir sangue para os pacientes Testemunhas de Jeová, nos casos de risco iminente de morte. Metodologia: Revisão integrativa baseada em artigos científicos encontrados nas bases de dados Lilacs, Pubmed e Scielo de acesso gratuito. Foram também analisados livros acadêmicos, normativas legais e decisões judiciais pertinentes ao tema. Resultados: Segundo o Código de Ética Médica é vedado ao médico não obter consentimento do paciente ou representante legal após o esclarecimento de um procedimento, com exceção dos casos de risco iminente de morte. O médico ainda deve executar alternativas que sejam autorizadas pelo paciente para evitar sua morte, não partindo diretamente para a intervenção não consensual. Discussão: A recusa à transfusão sanguínea está enraizada nas crenças religiosas dos TJ, por considerarem uma violação de mandamentos divinos. O médico deve respeitar a decisão informada do paciente, porém deve ponderar as implicações dessa escolha sobre a vida do indivíduo. Recentemente esse tema foi discutido pelo Superior Tribunal Federal (STF), que decidiu em favor da liberdade religiosa. Conclusão: Diante desse dilema, é fundamental ampliar o arsenal terapêutico disponível e fortalecer o diálogo dos médicos com os pacientes Testemunhas de Jeová, garantindo que suas crenças sejam respeitadas, sem comprometer a qualidade do cuidado recebido.
Diálogos do letramento literário com as artes nos anos iniciais: Reflexões e práticas docentes
Authors: Soares Emerson Andion Souza , Gaspar Débora do Socorro da Silva , Vasconcelos Thamires Fernanda C. de O.
Published: February 2025

O presente trabalho tem como objetivo mostrar como a alfabetização e o letramento são fundamentais na educação básica. Enquanto a alfabetização se concentra na decodificação, o letramento permite a compreensão do sentido dos textos dentro de um contexto social. A literatura é uma ferramenta poderosa para o desenvolvimento da leitura e da escrita, ajudando os alunos a se apropriarem da linguagem e a expressarem suas ideias. Em uma aula com uma turma do 3º ano, foi explorada a lenda do Curupira, conectando-a à preservação ambiental. A leitura coletiva da lenda e atividades criativas, como desenhos e tirinhas, foram propostas para estimular a reflexão sobre a importância da natureza. A atividade realizada proporcionou um espaço para que os alunos expressassem suas compreensões e promovesse uma conexão significativa com suas vivências, destacando a importância da oralidade e da literatura amazônica no contexto educacional.
Prevalencia y grado de fluorosis dental en escolares de 8 a 10 años. Ferreñafe, Lambayeque - Perú
Authors: Mori-Yampufe Janet Luz , Perona-Miguel de Priego Guido Alberto
Published: February 2025

Objetivo: Determinar la prevalencia y grado de fluorosis en escolares de 8 a 10 años. Material y métodos: Investigación cuantitativa, de tipo descriptivo, corte transversal y prospectivo, con una muestra de 160 estudiantes de ambos sexos, entre 8 a 10 años, de la Institución Educativa de Ferreñafe, los datos se recopilaron a través de evaluación clínica y análisis, con una ficha de registro en base a los Criterios de Dean para fluorosis dental. Resultados: El 85% de los participantes observados tenían fluorosis, además, 56,3% de los niños presentaron fluorosis de grado leve, el 25% de grado moderado, el 13,8% fluorosis severa. La fluorosis leve predominó en ambos sexos con 58,2% en el sexo femenino y el 56,3% en el sexo masculino, y en un 58,7%, 55,7% y 50% en los niños de 8 años, 9 años y 10 años respectivamente. Conclusiones: La prevalencia de fluorosis fue alta, el sexo femenino tuvo una mayor prevalencia de fluorosis severa y en el sexo masculino el grado de fluorosis moderada, además la prevalencia de fluorosis disminuyó con la edad, por eso resalta la necesidad de evaluar los niveles de fluoruros en la comunidad, considerando enfoques integrales en la salud bucal y estrategias adaptadas a cada entorno.
Salud Mental: Reflexiones clínicas y determinantes sociales
Authors: Admin Admin
Published: February 2025

La segunda entrega del Simposio sobre Salud Mental durante períodos críticos en la vida de comunidades y diversos grupos humanos, cubre inicialmente experiencias dentro de la llamada “Psiquiatría de enlace”, i.e., la provisión de consultas psiquiátricas en el diagnóstico y manejo de complicaciones emocionales/conductuales en pacientes médicos y/o quirúrtgico de hospitales generales, durante la pandemia.
THE IMPACT OF CORPORATE SOCIAL RESPONSIBILITY ON LOCALS; THE CASE OF THE HOSPITALITY SECTOR IN CRETE
Authors: Apostolakis Alexandros , Daniil Corina , Kourgiantakis Markos
Published: February 2025

The current paper focuses upon the impact of Corporate Social Responsibility (CSR) on locals’ opinions regarding the hospitality sector in Crete. The main research aim driving the current study is to evaluate respondents’ (locals’) willingness to pay a premium for CSR activities in the hospitality sector. The paper utilizes primary data collected through a survey questionnaire in the region of Crete. In total, researchers collected 400 survey questionnaires over a course of three months during the pandemic period. The paper utilized a logistic regression framework. According to the results, respondents that were highly familiar with CSR were also very positive in supporting CSR actions. Whereas gender does not seem to exert a particularly strong influence on individual willingness to pay a CSR premium, yet once the analysis distinguishes between different premium levels, females were found more likely to contribute at lower amounts, as compared to their male counterparts. The results indicate that businesses should adopt and adapt their CSR practices to new business discourses.
CONFORMITY ASSESSMENT OF SPARE PARTS OF WHEELED VEHICLES
Authors:   SE “State Road Transport Research Institute”, Gutarevich Serhii , Shkurotianyi Iurii , Ponomarova Iuliia , Nizheborska Zhanna ,   SE “State Road Transport Research Institute”
Published: February 2025

The article analyzes accidents involving wheeled vehicles, the impact of compliance of wheeled vehicle compo-nents with technical requrements on the safety of operation of vehicles on which such components are used. The segment of the spare parts market is considered depending on the participation of vehicle manufacturers in their design and production. The article presents the results of statistical processing of expert assessments of car owners, rep-resentatives of car service and specialists in the field of spare parts sales in Ukraine concerning the use of these spare parts, which belong to different segments, throughout the life cycle of an average wheeled vehicle. The international legal framework for technical regulation in the field of spare parts for vehicles, including the main provisions of the 1998 Global Agreement and the 1958 Geneva Agreement is considered. The results of the analysis of the requirements of international technical regulations that establish requirements for spare parts of vehicles when as-sessing their compliance are presented. In connection with the trends in changes in the design of modern vehicles, based on the analysis carried out, recommen-dations are given regarding the improvement of such requirements. Keywords: Spare parts for wheeled vehicles, road traffic accidents, safety, conformity assessment, UN regulation, global technical regulation, technical regulation.
Feeder-cell-free system for ex vivo production of natural killer cells from cord blood hematopoietic stem and progenitor cells
Authors: Martin Corredera Marta , Paillet Juliette , Gaudeaux Pierre , Blein Tifanie , Sadek Hanem , Rault Pauline , Berriche Asma , Roche-Naude Jeanne , Lagresle-Peyrou Chantal , Soheili Tayebeh-Shabi , André Isabelle , Moirangthem Ranjita Devi , Negre Olivier
Published: February 2025

Introduction

Natural Killer (NK) cells hold significant promise as therapeutic agents in immuno-oncology due to their ability to target and eliminate cancerous and infected cells without causing graft-versus-host disease or cytokine release syndrome. However, the limited availability of robust, scalable methods for generating clinical-grade NK cells remains a limiting factor to broader clinical application.

Methods

Here we report the development of a novel feeder-cell-free culture system optimized for producing NK cells from cord blood-derived CD34+ hematopoietic stem and progenitor cells (HSPCs). Our method eliminates the need for feeder cells while achieving high yields of NK cells that exhibit unique marker expression and cytotoxic functions. Cord blood CD34+ HSPCs were cultured in our established hDLL 4 culture system and generated large numbers of human T lymphoid progenitors (ProTcells) in 7 days. ProTcells were further cultured in a hDLL4-free, feeder-cell-free system for NK cell differentiation and supplemented with cytokines. Following a 7- or 14-day culture, this method produced highly pure NK cell populations (>90% CD3–CD56+).

Results

Flow and mass cytometric analysis confirmed the expression of activating receptors, transcription factors (ID2, T-bet) and cytotoxic molecules (perforin, granzyme A/B), all essential for ProT-NK cell functionality. These cells are in an immature state, indicated by the absence of maturation markers (CD16, KIRs). Functional assays demonstrated that these ProT-NK cells are capable of degranulation and cytokines production (TNFα) upon stimulation with K562 target cells and showed cytotoxicity against K562 cells superior to that of Peripheral Blood (PB)-NK. In NSG-Tg(hIL-15) mice, ProT-NK cells colonize bone marrow, the liver, and the spleen and persist and mature in bone marrow for at least 9 days post-injection. Compared to ProT-NK D21, ProT-NK D14 was superior in functional and homing potential. In vivo, an anti-tumor assay that uses a subcutaneous K562 model has demonstrated the anti-tumor potential of ProT-NK cells.

Discussion

Our ex vivo culture process supports scalable ProT-NK cell production in high yields, reducing dependency on feeder cells and mitigating contamination risks. Our findings demonstrate the feasibility of generating large, functional NK cell populations from HSPCs isolated from readily available cord blood sources and offer an efficient alternative to PB-NK cell therapies.
Inhibition of EETosis with an anti-citrullinated histone antibody: a novel therapeutic approach for eosinophilic inflammatory disorders
Authors: Zwiers Eline , Montizaan Daphne , Kip Annemarie , Waaijenberg Kelsy , Fichtinger Paul S. , Mathur Sameer K. , Fujioka Yuki , Ueki Shigeharu , van Es Helmuth , Chirivi Renato G. S. , Meldrum Eric , van der Linden Maarten
Published: February 2025

Eosinophils are a subset of granulocytes that protect the host against fungal and parasitic infection through secretion of their granular contents. In response to specific stimuli, eosinophils also undergo a type of lytic cell death, referred to as eosinophil extracellular trap (EET)-associated cell death (EETosis), where histone citrullination facilitates chromatin decondensation, cell rupture and release of pro-inflammatory, decondensed chromatin into the extracellular environment as EETs. In this study, we show the abundant presence of eosinophils and citrullinated histones in nasal polyp tissue of patients with eosinophilic chronic rhinosinusitis (ECRS). Using live imaging microscopy on purified human eosinophils, we demonstrate that physiologically relevant stimuli induce release of citrullinated EETs and the marker of eosinophil activation galectin-10. While the kinetics of release of EETs and galectin-10 are similar, inhibitors of citrullination block EETosis in a dose dependent manner but fail to inhibit galectin-10 release. The importance of citrullination is further exemplified with CIT-013, a monoclonal antibody specific for citrullinated histones H2A and H4. CIT-013 potently inhibits release of EETs (half-maximal inhibitory concentration of 2.5 nM) without inhibiting other eosinophil functions such as degranulation, adhesion, superoxide production and induction of chemokine expression. Together, this study provides new insights into the requirement of protein arginine deiminase 4 (PAD4) for EETosis, differentiates requirements of EETosis from galectin-10 release, and identifies a novel therapeutic approach for EETosis inhibition by targeting citrullinated histones in eosinophil-driven diseases such as ECRS.
Defining the value proposition in diagnostic technology: challenges and opportunities for its understanding and development – a review with a multiperspective reflective analysis
Authors: Soukup Tayana , Zamora-Talaya Bernarda , Bahadori Shayan , Luxardo Rosario , Kierkegaard Patrick , Butt Omar , Kettley-Linsell Hannah , Savva Katerina-Vanessa , Micocci Massimo , Zhou Shanshan , Newman Simon , Walne Simon , Peters Christopher J. , Gordon Adam , Ni Melody , Buckle Peter , Hanna George B. ,   NIHR HRC IVD PPIE Team
Published: February 2025

Background

The Value Proposition (VP) in diagnostic technology serves as a “positioning statement” outlining the unique benefits, costs, and differentiation an innovation under development offers to healthcare organizations and its ability to effectively deliver these advantages in comparison to current interventions in the market. Despite its significance however, VP lacks a universally accepted definition, which is compounded by the diversity of technologies, their applications, and the varying needs of stakeholders. This paper aims to address this gap by offering a detailed conceptual analysis, revised definition of VP, and actionable recommendations for advancing VP development.

Methodology

We conducted a targeted narrative review, focusing on literature explicitly defining VPs in diagnostic technologies. Using Ovid’s Medline and Embase databases, we identified 19 relevant papers, of which only 5 provided explicit VP definitions. Our analysis incorporated principles of team science, encompassing reflective and thematic analyses of (1) interdisciplinary co-author discussions enabling us to weave together diverse insights into a cohesive exploration of the topic, and (2) MTech’s publicly available set of anonymised responses from NHS Associates, to capture the perspectives of the decision-makers and further enhance depth and breadth of our discourse.

Results and discussion

Our findings highlight the multifaceted nature of VP and its primary hurdles: inadequate identification of unmet needs and insufficient recognition of key stakeholders. We synthesized the evolution of VP definitions and explored the importance of unmet needs in their development, guided by frameworks, such as the Health Technology Navigation Pathway Tool, to ensure VPs meet both the pragmatic and aspirational goals of the healthcare. Thematic insights revealed opportunities for addressing these barriers through implementation science and collaborative strategies. This multi-perspective approach provided a conceptual examination of VP, enabling integration of varied viewpoints and insights.

Conclusion

By employing team science principles and reflective analysis, we introduced a revised definition of VP and a set of actionable recommendations to guide VP development in diagnostics. These findings highlight the importance of addressing stakeholder diversity, unmet needs, and the intricacies of blending interdisciplinary perspectives to advance the field.
Molecular phylogenetic analyses reveal multiple long-distance dispersal events and extensive cryptic speciation in Nervilia (Orchidaceae), an isolated basal Epidendroid genus
Authors: Gale Stephan W. , Li Jihong , Suddee Somran , Traiperm Paweena , Peter Craig I. , Buruwate Tomas , Crain Benjamin J. , McCormick Melissa K. , Whigham Dennis F. , Musthofa Arni , Gogoi Khyanjeet , Ito Katsura , Minamiya Yukio , Fukuda Tatsuya , Landrein Sven , Yukawa Tomohisa
Published: February 2025

Introduction

The terrestrial orchid genus Nervilia is diagnosed by its hysteranthous pattern of emergence but is nested among leafless myco-heterotrophic lineages in the lower Epidendroideae. Comprising ca. 80 species distributed across Africa, Asia and Oceania, the genus remains poorly known and plagued by vague and overlapping species circumscriptions, especially within each of a series of taxonomically intractable species complexes. Prior small-scale, exploratory molecular phylogenetic analyses have revealed the existence of cryptic species, but little is otherwise understood of origin, the scale and timing of its biogeographic spread, or the palaeoclimatic factors that have shaped its ecology and given rise to contemporary patterns of occurrence.

Methods

Here, we sample widely throughout the generic range, including 45 named taxa and multiple accessions referable to several widespread ‘macrospecies’, as well as material of equivocal identity and probable undescribed status, for the first time enabling an evaluation of taxonomic boundaries at both species and sectional level. Using nuclear (ITS) and plastid (matK, trnL-F) sequence data, we conduct phylogenetic (maximum parsimony and Bayesian inference) and ancestral area analysis to infer relationships and resolve probable origin and colonisation routes.

Results

The genus is strongly supported as monophyletic, as are each of its three sections. However, the number of flowers in the inflorescence and other floral characters are poor indicators of sectional affinity. Dated ancestral area analysis supports an origin in Africa in the Early Oligocene, with spread eastwards to Asia occurring in the Late Miocene, plausibly via the Gomphotherium land bridge at a time when it supported woodland and savanna ecosystems.

Discussion

Taxonomic radiation in Asia within the last 8 million years ties in with dramatic Himalayan-Tibetan Plateau uplift and associated intensification of the Asia monsoon. Multiple long-range migrations appear to have occurred thereafter, as the genus colonised Malesia and Oceania from the Pliocene onwards. The bulk of contemporary species diversity is relatively recent, potentially explaining the ubiquity of cryptic speciation, which leaves numerous species overlooked and unnamed. Widespread disjunct species pairs hint at high mobility across continents, extinction and a history of climate-induced vicariance. Persistent taxonomic challenges are highlighted.
Prenatal alcohol exposure before pregnancy awareness: a thematic analysis of online forum comments and misinformation
Authors: Frennesson Nessie Felicia , Merouani Youssouf , Barnett Julie , Attwood Angela , Zuccolo Luisa , McQuire Cheryl
Published: February 2025

Background

Many women consume alcohol while pregnant before they are aware of the pregnancy, raising concerns about potential harms to the developing fetus. Official guidelines in the United Kingdom recommend abstinence throughout pregnancy, and many women turn to online forums for reassurance and information. However, online forums can also become a source of misinformation, potentially increasing confusion and anxiety among women who have consumed alcohol before pregnancy awareness. This study explored discussions about alcohol consumption before pregnancy awareness on Mumsnet to understand the nature of peer response and assess the accuracy of information against official health guidelines and the scientific literature.

Methods

A thematic analysis was conducted on 71 thread starts and 1,281 comments from Mumsnet. Data was collected via web scraping, followed by manual screening. Themes were identified, and information-sharing posts were fact-checked against scientific evidence and guidelines.

Results

Two overarching themes with five sub-themes emerged: “Type of reassurance offered”, where users offered reassurance to alleviate worries, and “Reactions to reassurance”, where some users appeared reassured while others did not. While many found reassurance, fact-checking revealed that the majority of the information was inaccurate, often underestimating the risks of prenatal alcohol exposure.

Conclusions

Online forums may provide a source of peer support to those who have consumed alcohol before pregnancy awareness but frequently spread misinformation about alcohol use in early pregnancy. Health professionals should ensure pregnant women have access to accurate information alongside appropriate support to reduce anxiety and avoid the spread of harmful misinformation.
Enhancing constituent estimation in nucleic acid mixture models using spectral annealing inference and MS/MS information
Authors: Tomono Taichi , Hara Satoshi , Iida Junko , Washio Takashi
Published: February 2025

Mass spectrometry (MS) is a powerful analytical technique employed for a variety of applications including drug development, quality assurance, food inspection, and monitoring environmental pollutants. Recently, in the production of actively developed antibody and nucleic acid pharmaceuticals, impurities with various modifications have been generated. These impurities can lead to a decrease in drug stability, pharmacokinetics, and efficacy, making it crucial to distinguish between them. We previously modeled mass spectrometry for each possible number of constituents in a sample, using parameters such as monoisotopic mass and ion counts, and employed stochastic variational inference to determine the optimal parameters and the maximum posterior probability for each model. By comparing the maximum posterior probabilities among models, we selected the optimal number of constituents and inferred their corresponding monoisotopic masses and ion counts. However, MS spectra are sparse and predominantly flat, which can lead to vanishing gradients when using simple optimization techniques. To solve this problem, using MCMC as in our previous studies would take a very long time. To address this difficulty, in this study, we blur the comparative spectra and gradually reduce the blur to prevent vanishing gradients while inferring accurate values. Furthermore, we incorporate MS/MS spectra into the model to increase the amount of information available for inference, thereby improving the accuracy of parameter inference. This modification improved the mass error from an average of 1.348 Da–0.282 Da. Moreover, the required time, even including the processing of additional five MS/MS spectra, was reduced to less than half.
Development and Test of a Self-Propelled Peanut Combine Harvester for Hilly and Mountainous Regions
Authors: Pan Liang , Yang Hongguang , Yu Zhaoyang , Shen Haiyang , Gu Man , Luo Weiwen , Wu Feng , Gu Fengwei , Ren Guiying , Hu Zhichao
Published: February 2025

Addressing the issue of complex terrain and small field plots in hilly and mountainous regions where large combine harvesters are not suitable, this paper presented the design and development of a semi-feed self-propelled peanut combine harvester. This harvester is characterized by its small size and flexible steering. Theoretical calculations were used to determine the structural parameters of the main working components. A three-factor, three-level orthogonal experimental design was implemented, focusing on forward speed, vibration frequency and picking roller rotational speed, as these parameters significantly influence operational performance. Through this experiment, regression models were established between the total loss rate, the broken pod rate, and these three above mentioned factors. Through multi-objective optimization, it was found that when the forward speed is 0.44 m/s, the picking roller rotational speed is 350 rpm, and the vibration frequency is 6.4 Hz, the total loss rate and broken pods rate of the harvester are the lowest. Validation experiments were conducted under this parameter combination, with the total loss rate and broken pods rate being effectively reduced to 3.21% and 0.85%, respectively. The experiments proved that this harvester meets the requirements for mechanized peanut harvesting in hilly and mountainous regions.