bluuebunny/crossref_metadata_2025 huggingface dataset:
Arabidopsis thaliana Plants’ Overexpression of the MYB Transcription Factor VhMYB60 in the Face of Stress Hazards Enhances Salt and Cold Tolerance
Authors: Chen Zhe , Wang Jinghan , Li Wenhui , Chen Xiang , Zhao Changjia , Guo Yanbo , Li Yingnan , Chen Zhuo , Li Xingguo , Han Deguo
Published: February 2025

‘Beta’ (Vitisriparia × V. labrusca) is a vine fruit tree of the genus Vitis which is a cross between American and riparian grapes. In the current situation of grape production in northern regions, cold, drought, and salinity are important bottlenecks restricting its development, while some grape rootstocks with excellent traits show the disadvantage of poor resilience. ‘Beta’ (Vitis riparia × V. labrusca), one of the most extensively utilized rootstocks in viticulture, has demonstrated remarkable resilience to adverse conditions. However, the mechanisms by which ‘Beta’ rootstocks resist abiotic stresses are unknown and need to be further investigated. In this study, we successfully isolated and cloned a novel MYB transcription factor, VhMYB60, from the ‘Beta’ grapevine. This factor spans 972 base pairs and encodes a protein comprising 323 amino acids. Subcellular localization studies revealed that VhMYB60 is predominantly expressed within the nucleus. Furthermore, tissue-specific expression analysis demonstrated that VhMYB60 is more abundantly expressed in the mature leaves and roots of the grape plant. Further studies showed that salt and cold stress notably increased VhMYB60 gene expression in both mature leaves and grape roots. Compared with the control, Arabidopsis thaliana (Arabidopsis) plants molecularly modified to overexpress VhMYB60 exhibited enhanced salt and cold resistance and improved survival rates. Moreover, notable changes were detected in chlorophyll, malondialdehyde (MDA), proline, peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) levels. Concurrently, the expression levels of structural genes that are positively correlated with resistance to adversity stress were markedly elevated in Arabidopsis plants that overexpress VhMYB60. Consequently, VhMYB60 may serve as a pivotal transcription factor in the regulation of ‘Beta’ resistance.
Selenium-Containing Nanoformulations Capable of Alleviating Abiotic Stress in Plants
Authors: Tsivileva Olga
Published: February 2025

Climate changes cause various types of abiotic stress in plants, thus affecting plant growth and causing decline in yield. An urgent need exists to develop an environmentally friendly attitude based on principles of sustainable agriculture. Nanomaterials may improve plant growth and enhance crop productivity by handling the conditions considered stressful for plants in a sustainable and ecofriendly manner. Selenium (Se) has been put into the category of beneficial elements in plants. Se-enriched crops present a successful choice of dietary resource for Se-supplemented food and feed owing to their high bioavailability and accessibility. Researchers from distinct areas, including both nanoscience and plant science, should encourage emerging innovations that are linked with abiotic stress in crop production. The implementation of Se nanoparticles (SeNPs) is considered one of the predominating mechanisms by plants to ameliorate stressful conditions. Increasing evidence of earlier research revealed that SeNPs could enhance plant growth and development, nutrient bioavailability, soil fertility, and stress response while maintaining environmental safety. Meanwhile, some earlier studies reported that SeNPs might have a multilateral influence on plants dependent on diverse Se nanomaterial traits, doses, and plant species. More efforts are required to enhance the knowledge of how SeNPs impact crops exposed to different abiotic detrimental factors. In light of contemporary research challenges linked to SeNPs and the prolonged application of Se nanomaterials to plants, the aim of this review is elucidating the principal fruitful areas of SeNP exploration, comparisons with bulk Se, insights into mechanisms of abiotic stress alleviation in plants, existing research uncertainties, and practical challenges for SeNP applications under varying environments.
The Gut Microbiota Metabolite Butyrate Modulates Acute Stress-Induced Ferroptosis in the Prefrontal Cortex via the Gut–Brain Axis
Authors: Wang Zhen , Ma Xiaoying , Shi Weibo , Zhu Weihao , Feng Xiaowei , Xin Hongjian , Zhang Yifan , Cong Bin , Li Yingmin
Published: February 2025

Stress has been implicated in the onset of mental disorders such as depression, with the prefrontal cortex (PFC) playing a crucial role. However, the underlying mechanisms remain to be fully elucidated. Metabolites secreted by intestinal flora can enter the bloodstream and exert regulatory effects on the body. Consequently, this study aims to investigate the molecular mechanisms by which gut flora influences ferroptosis in PFC neurons, thereby affecting depression-like behavioral changes in mice subjected to acute stress. Initially, we established a mouse model of acute restraint stress (3-day duration) and verified that stress-induced ferroptosis of PFC neurons contributed to depression-like behavioral alterations in mice, as evidenced by morphological, behavioral, and molecular biology assessments. Subsequently, through fecal microbiota transplantation (FMT) experiments, we established a significant correlation between gut microbiota and ferroptosis of PFC neurons in acute stress-exposed mice. 16S rDNA sequencing identified butyric acid-producing bacteria, specifically g_Butyricimonas and its primary metabolite, butyric acid, as critical regulators of ferroptosis in PFC neurons in acutely stressed mice. Furthermore, the intervention of butyrate demonstrated its potential to ameliorate damage to the intestinal and blood–brain barriers in these mice. This intervention also mitigated depression-like behaviors induced by ferroptosis of PFC neurons by alleviating systemic inflammatory responses. The findings of this study indicate that acute stress-induced ferroptosis of PFC neurons plays a critical role in depression-like behavioral changes in mice. Additionally, the gut microbiota metabolite butyrate can modulate ferroptosis and depression-like behavioral changes through the gut–brain axis.
Bone Marrow Origin of Mammary Phagocytic Intraductal Macrophages (Foam Cells)
Authors: Barsky Sanford H. , Mcphail Krista , Wang Justin , Hoffman Robert M. , Ye Yin
Published: February 2025

Mammary intraductal macrophages (foam cells) in humans are the most commonly encountered cells in spontaneous breast nipple discharge, nipple aspirate fluid, and ductal lavage, yet their origin remains unproven. These cells, in both humans and murine model systems, increase in pregnancy, pseudopregnancy, and other conditions like proliferative fibrocystic disease and intraductal neoplasia, ductal carcinoma in situ (DCIS), where there is intraductal ectasia and obstruction. Previous immunocytochemical studies with macrophage (CD68, lysozyme), epithelial (cytokeratin, estrogen receptor), and myoepithelial (smooth muscle actin, CALLA, maspin) markers have indicated that intraductal foam cells are of macrophage lineage. These foam cells engage in phagocytosis of both endogenous and exogenous substances present within the ducts and are not proliferative. Although it has been suggested that foam cells could derive from tissue-specific and niche-specific precursors or circulating monocytes, to date no experimental nor clinical studies have provided direct proof of their origin. In this study, we provide evidence in both human and murine bone marrow transplant studies that intraductal foam cells are bone marrow-derived. We first studied a registry of sex-mismatched bone marrow transplant recipients who later in life had undergone breast biopsies for either proliferative fibrocystic disease, DCIS, or gynecomastia, and studied these biopsies by XY chromosome fluorescence in situ hybridization (FISH) and informative microsatellite polymorphic markers. The intraductal foam cells were of bone marrow donor-origin. Then, in the experimental bone marrow transplant murine studies, donor marrow from female ROSA26 containing the lacZ reporter were transplanted into either irradiated female recipient transgenic mice carrying the highly penetrant MMTV-pymT or FVB/N background mice, where induced pluripotent stem (iPS) cells derived from tail vein fibroblasts of FVB/N-Tg(MMTV-PyVT)634Mul/J mice were subsequently injected into their mammary fat pads. In all of the transplanted recipient mice, the intraductal foam cells expressed the β-galactosidase (lacZ) reporter and also co-expressed markers of myeloid–macrophage lineage. The number of donor-derived intraductal foam cells increased in pseudopregnancy 5-fold and in intraductal neoplasia 10-fold. Although macrophages of different origins and lineages are undoubtedly present within both the murine and human breasts, those macrophages that qualify as phagocytic intraductal foam cells are bone marrow-derived.
Synthesis, Antimicrobial Activity, and Tyrosinase Inhibition by Multifunctional 3,4-Dihydroxy-Phenyl Peptidomimetics
Authors: Wavhal Deepak S. , Koszelewski Dominik , Kowalczyk Paweł , Brodzka Anna , Ostaszewski Ryszard
Published: February 2025

The purpose of the present study was to evaluate the synergistic effect of two important pharmacophores, 3,4-dihydroxyphenyl and peptidomimetic moieties, as mushroom tyrosinase inhibitors and antimicrobial agents targeting specific strains of pathogenic bacteria. The 3,4-dihydroxybenzaldehyde (protocatechuic aldehyde) was found to be an effective inhibitor of tyrosinase activity, and due to the fact that it is a safe natural substance with such a scaffolded structure, it is likely that dihydroxyl-substituted phenolic derivatives can exhibit potent tyrosinase inhibitory activity. Series of peptidomimetics with an incorporated 3,4-dihydroxyphenyl scaffold was synthesized and characterized. The inhibitory effects of peptidomimetics on a mushroom tyrosinase were studied. The results showed that among the compounds, five of them showed higher inhibitory activity than the parent 3,4-dihydroxybenzyl aldehyde. In silico docking studies with mushroom tyrosinase (PDB ID 2Y9X) predicted possible binding modes in the enzymatic pocket for these compounds. Furthermore, the antimicrobial activities of peptidomimetics against selected Gram-positive and Gram-negative bacterial strains (E. coli, A. baumannii, P. aeruginosa, E. cloacae, and S. aureus) were investigated. The results showed that all tested peptidomimetics have antimicrobial activities (MIC values from 0.25 to 4.0 μM) comparable with those observed for the commonly used antibiotics (ciprofloxacin, bleomycin, and cloxacillin). Notably, all evaluated compounds demonstrated significant activity against E. coli and S. aureus strains, which are primary sources of infections resulting in numerous fatalities. Additionally, the cytotoxicity of sixteen derivatives was assessed using the MTT assay on BALB/c3T3 mouse fibroblast cell lines. Cytotoxicity analyses indicated that the tested substances have a similar or reduced impact on cell proliferation compared to commonly utilized antibiotics within the range of therapeutic doses. This study presents the potential of peptidomimetics with 3,4-dihydroxyphenyl scaffolds could be beneficial for developing novel tyrosinase inhibitors and new potent food preservatives or cosmetic additives.
DynGraph-BERT: Combining BERT and GNN Using Dynamic Graphs for Inductive Semi-Supervised Text Classification
Authors: Perin Eliton Luiz Scardin , Souza Mariana Caravanti de , Silva Jonathan de Andrade , Matsubara Edson Takashi
Published: February 2025

The combination of Bidirecional Encoder Representations from Transformers (BERT) and Graph Neural Networks (GNNs) has been extensively explored in the text classification literature, usually employing BERT as a feature extractor combined with heterogeneous static graphs. BERT transfers information via token embeddings, which are propagated through GNNs. Text-specific information defines a static heterogeneous graph. Static graphs represent specific relationships and do not have the flexibility to add new knowledge to the graph. To address this issue, we build a tied connection between BERT and GNN exclusively using token embeddings to define the graph and propagate the embeddings, which can force the BERT to redefine the GNN graph topology to improve accuracy. Thus, in this study, we re-examine the design spaces and test the limits of what this pure homogeneous graph using BERT embeddings can achieve. Homogeneous graphs offer structural simplicity and greater generalization capabilities, particularly when integrated with robust representations like those provided by BERT. To improve accuracy, the proposed approach also incorporates text augmentation and label propagation at test time. Experimental results show that the proposed method outperforms state-of-the-art methods across all datasets analyzed, with consistent accuracy improvements as more labeled examples are included.
Identification of Necrophagous Beetles (Coleoptera) Using Low-Resolution Real-Time PCR in the Buffer Zone of Kampinos National Park
Authors: Malewski Tadeusz , Leszczyńska Katarzyna , Borzuchowska Katarzyna Daria , Sierakowski Maciej , Oszako Tomasz , Nowakowska Justyna Anna
Published: February 2025

Forensic entomology is concerned with the study of arthropods that assist in criminal investigations. Among them, necrophagous beetles (Coleoptera) play a crucial role as they are found in all stages of decomposition of corpses, which makes them valuable for forensic analysis. Accurate species identification is crucial for the effective use of entomological evidence. Efficient, cost-effective, and rapid methods are essential for this purpose. In the present study, we developed a novel assay that enables simple, inexpensive, and rapid identification of necrophagous beetle species. Using two primer sets targeting the cytochrome c oxidase 1 (COI) gene and analyzing the resulting SYBR Green I melting curves, we were able to identify fourteen beetle species: Aleochara curtula (Goeze, 1777), Anoplotrupes stercorosus (Scriba, 1791), Creophilus maxillosus (Linnaeus, 1758), Dermestes undulatus (Brahm, 1790), Hister unicolor (Linnaeus, 1758), Margarinotus brunneus (Fabricius, 1775), Necrodes littoralis (Linnaeus, 1758), Nicrophorus investigator (Zetterstedt, 1824), Ontholestes murinus (Linnaeus, 1758), Oiceoptoma thoracicum (Linnaeus, 1758), Philonthus cognatus (Stephens, 1832), Saprinus planiusculus (Motschulsky, 1849), Silpha tristis (Illiger, 1798), and Thanatophilus rugosus (Linnaeus, 1758). Data on the melting temperature of the amplicon can be obtained within 60–90 min after DNA extraction. This study expands the understanding of the species composition of necrophagous Coleoptera, which is crucial for forensic entomology methods based on insect succession. Furthermore, it provides the first comprehensive data on necrophagous beetles in the vicinity of Kampinos National Park.
Fall Armyworm-Induced Secondary Metabolites in Sorghum Defend Against Its Attack
Authors: Zhao Juan-Ying , Lu Qi , Sun Jiang , Sun Li-Yuan , Ma Ruiyan , Wang Yuanxin , Hu Jun , Wang Huiyan , Zhang Yizhong , Jia Dong , Yang Jun
Published: February 2025

The fall armyworm (FAW), Spodoptera frugiperda, is one of the major agricultural pests that has invaded China. The FAW is a polyphagous insect with the gramineous crop sorghum being a key host plant. However, the basis of sorghum’s chemical defense against FAW feeding is still unclear. In this study, we investigated the potential defensive mechanism of sorghum against this insect species. It was found that FAW larvae preferred maize over sorghum, the selection and damage rates for sorghum plants by larvae were significantly lower than those of maize plants, and feeding on sorghum restricted larval weight. The non-target metabolomics revealed that the feeding of FAW larvae altered the plant secondary metabolite spectra in maize and sorghum, resulting in species-specific differential secondary metabolites (DSMs). Of these, 19 DSMs were specific in maize, and 51 in sorghum, and only 6 were found in both species. Two-choice and no-choice feeding assays found that gambogenic acid and chimonanthine, two DSMs unique to sorghum, were found to deter larval feeding and decrease the larval weight. These findings reveal that the defense of sorghum against FAW is regulated by changing the response spectra of secondary metabolites and that the induced metabolites have a defensive function by acting as antifeedants, which provides new insights into employing bioactive plant compounds against polyphagous insects.
On the Study of Joint YOLOv5-DeepSort Detection and Tracking Algorithm for Rhynchophorus ferrugineus
Authors: Wu Shuai , Wang Jianping , Wei Wei , Ji Xiangchuan , Yang Bin , Chen Danyang , Lu Huimin , Liu Li
Published: February 2025

The Red Palm Weevil (RPW, Rhynchophorus ferrugineus) is a destructive pest of palm plants that can cause the death of the entire plant when infested. To enhance the efficiency of RPW control, a novel detection and tracking algorithm based on the joint YOLOv5-DeepSort algorithm is proposed. Firstly, the original YOLOv5 is improved by adding a small object detection layer and an attention mechanism. At the same time, the detector of the original DeepSort is changed to the improved YOLOv5. Then, a historical frame data module is introduced into DeepSort to reduce the number of target identity (ID) switches while maintaining detection and tracking accuracy. Finally, an experiment is conducted to evaluate the joint YOLOv5-DeepSort detection and tracking algorithm. The experimental results show that, in terms of detectors, the improved YOLOv5 model achieves a mean average precision (mAP@.5) of 90.1% and a precision (P) of 93.8%. In terms of tracking performance, the joint YOLOv5-DeepSort algorithm achieves a Multiple Object Tracking Accuracy (MOTA) of 94.3%, a Multiple Object Tracking Precision (MOTP) of 90.14%, reduces ID switches by 33.3%, and realizes a count accuracy of 94.1%. These results demonstrate that the improved algorithm meets the practical requirements for RPW field detection and tracking.
Development and Usability Evaluation of Inventive and Repurposable Children’s Furniture
Authors: Phuah Zhi Yuan , Ng Poh Kiat , Tay Chai Hua , Lim Boon Kian , Liew Kia Wai , Chong Peng Lean
Published: February 2025

This study developed inventive and repurposable children’s furniture to improve functionality and extend product lifespan. Unlike typical cribs which serve a single purpose, the proposed design supports multiple functions: crib, cushioned chair, highchair, walker, toilet attachment, pull-up bar, and bed safety rail. Specific dimensions were established, and the correct material selections were made for the selected concept. This was finalised using Autodesk Inventor 2019 that was used for stress analysis, and material optimisation. Usability tests were conducted to compare the proposed invention with single-function products. These tests were discussed in terms of task completion time, space-saving ability, survey feedback, and a REBA of musculoskeletal risk. It was found that the proposed design could be repurposed in a shorter time and save more space. A majority of the survey participants agreed that it performed well in terms of repurposability, design, space-saving ability, usability, comfort, sustainability, and safety. Additionally, the proposed design is cheaper compared to single-function products. Thus, creating inventive and repurposable children’s furniture can contribute to reducing waste and extending the lifespan of children’s furniture through innovative, cost-effective design solutions.
Determinants of Longitudinal Changes in Exercise Blood Pressure in a Population of Young Athletes: The Role of BMI
Authors: Battista Francesca , Vecchiato Marco , Chernis Kiril , Faggian Sara , Duregon Federica , Borasio Nicola , Ortolan Sara , Pucci Giacomo , Ermolao Andrea , Neunhaeuserer Daniel
Published: February 2025

AIM: Higher exercise blood pressure in adults correlates with many cardiometabolic markers. The aim of this study was to investigate the main determinants of longitudinal variations in exercise blood pressure in young athletes. METHODS: A longitudinal retrospective study was conducted on adolescent athletes who underwent at least two sport-related pre-participation screening visits, including exercise testing with a standardized incremental ramp protocol on treadmill. Blood pressure was assessed at rest (SBPrest), at the 3rd minute of exercise (SBP3min), and at peak exercise (SBPpeak). Predictors of blood pressure response (i.e., respective changes vs. baseline (Δ)) were determined by multivariate regression models after adjustment for age, sex, follow-up duration, related baseline SBP values, characteristics of sport, and ΔBMI. RESULTS: A total of 351 young athletes (mean age at baseline 13 ± 2 years, 54% boys, average follow-up duration 3.4 ± 2.2 years) were enrolled. BMI increased by 1.5 ± 1.8 kg/m2 (p < 0.001) during follow-up. At baseline, mean SBPrest was 103 ± 14 mmHg, mean SBP3min 124 ± 18 mmHg, and mean SBPpeak 154 ± 23 mmHg. A significant between-visit increase in SBPrest (ΔSBPrest 7.0 ± 17.4 mmHg; p < 0.001), ΔSBP3min (4.8 ± 11 mmHg, p < 0.001), and ΔSBPpeak (11.7 ± 24 mmHg, p < 0.001) was observed. ΔSBP3min was significantly predicted by male sex (p < 0.01), baseline BMI (p < 0.01), ΔBMI (p < 0.01), and number of practiced sports (p < 0.05), whereas ΔSBPpeak was positively predicted by male gender (p < 0.01), baseline BMI (p < 0.05), and ΔBMI (p < 0.01) and negatively by baseline resting heart rate (p < 0.01). In a logistic regression model, ΔBMI was the only independent determinant of passing from a lower to an upper quartile of SBP3min (p < 0.001), while ΔBMI and male sex were independent determinants of moving to a higher quartile of SBPpeak (p < 0.001). CONCLUSIONS: Increase in BMI during development and male sex are independent determinants of the increase in exercise blood pressure, both at light and maximal intensity, in a population of adolescent athletes.
The BG Study Part 1 (Bergisch Gladbach): Development of a Prototype Coronary Artery Disease Risk Score Incorporating Peripheral Vascular Parameters—Preliminary Insights for Future CAD Risk Prediction Models in Vascular Patients
Authors: Aras Tuna , Tayeh Mahmoud , Aswad Adel , Sharkawy Mohamed , Almuzakki Zaki , Dorweiler Bernhard , Majd Payman
Published: February 2025

Background: Peripheral vascular parameters may provide valuable insights into coronary artery disease (CAD) risk stratification. This study aimed to develop a CAD risk score by integrating carotid duplex velocities, ankle–brachial index (ABI), and clinical history into a point-based model. Methods: We analyzed data from 902 cardiology patients, of whom 592 (65.6%) had confirmed CAD based on coronary angiography. Peripheral vascular assessments included carotid duplex ultrasonography and ABI measurements. Predictors were identified through multivariate logistic regression, addressing multicollinearity and interaction effects. A point-based scoring system was developed using statistically significant variables and evaluated via receiver operating characteristic (ROC) analysis. Results: Key predictors included external carotid artery velocities, ABI, carotid stenosis, chronic kidney disease (CKD) stage, smoking history, diabetes, hypertension, and age. The scoring system demonstrated moderate discriminative ability (AUC: 0.683) and high sensitivity (97%) for detecting CAD-positive cases but lower specificity (11%) for CAD-negative cases. Patients were stratified into risk categories, with an optimal threshold of ≥7 points maximizing the F1 score. Conclusions: This novel scoring system highlights the clinical relevance of integrating peripheral vascular assessments into CAD risk models. While its high sensitivity ensures robust detection of CAD-positive patients, future multicenter studies are needed to improve specificity and validate its broader clinical utility.
Unveiling the Therapeutic Potential of the Second-Generation Incretin Analogs Semaglutide and Tirzepatide in Type 1 Diabetes and Latent Autoimmune Diabetes in Adults
Authors: Infante Marco , Silvestri Francesca , Padilla Nathalia , Pacifici Francesca , Pastore Donatella , Pinheiro Marcelo Maia , Caprio Massimiliano , Tesauro Manfredi , Fabbri Andrea , Novelli Giuseppe , Alejandro Rodolfo , De Lorenzo Antonino , Ricordi Camillo , Della-Morte David
Published: February 2025

Type 1 diabetes mellitus (T1D) is a chronic autoimmune disease caused by the immune-mediated destruction of insulin-producing pancreatic beta cells, resulting in the lifelong need for exogenous insulin. Over the last few years, overweight and obesity have recently emerged as growing health issues also afflicting patients with T1D. In this context, the term “double diabetes” has been coined to indicate patients with T1D who have a family history of type 2 diabetes mellitus (T2D) and/or patients with T1D who are affected by insulin resistance and/or overweight/obesity and/or metabolic syndrome. At the same time, the use of second-generation incretin analogs semaglutide and tirzepatide has substantially increased on a global scale over the last few years, given the remarkable clinical benefits of these drugs (in terms of glucose control and weight loss) in patients with T2D and/or overweight/obesity. Although the glucagon-like peptide-1 (GLP-1) receptor agonists and the novel dual GIP (glucose-dependent insulinotropic polypeptide)/GLP-1 receptor agonist tirzepatide are currently not approved for the treatment of T1D, a growing body of evidence over the last few years has shown that these medications may serve as valid add-on treatments to insulin with substantial efficacy in improving glucose control, promoting weight loss, preserving residual beta-cell function and providing other beneficial metabolic effects in patients with T1D, double diabetes and latent autoimmune diabetes in adults (LADA). This manuscript aims to comprehensively review the currently available literature (mostly consisting of real-world studies) regarding the safety and therapeutic use (for different purposes) of semaglutide and tirzepatide in patients with T1D (at different stages of the disease), double diabetes and LADA.
Comorbidities, Endocrine Medications, and Mortality in Prader–Willi Syndrome—A Swedish Register Study
Authors: Giesecke Julia , Oskarsson Anna , Petersson Maria , Nordenvall Anna Skarin , Tettamanti Giorgio , Nordgren Ann , Höybye Charlotte
Published: February 2025

Background: Prader–Willi Syndrome (PWS) is a rare, genetic, multi-systemic disorder. Its main characteristics are muscular hypotonia, behavioral problems, intellectual disability, endocrine deficiencies, hyperphagia, and a high risk of morbid obesity and related comorbidities. This study aimed to investigate the rate of comorbidity, prescription of endocrine medications, and mortality in individuals with PWS compared to the general population. Methods: The association between PWS and outcomes were investigated in a matched cohort study of individuals born in the period of 1930–2018 with data from Swedish national health and welfare registers. Each individual was matched with 50 non-PWS comparisons. The associations between PWS, outcomes and prescribed endocrine medications were estimated through Cox proportional hazard models, presented as Hazard Ratios (HR) with 95% Confidence Intervals (CIs). Results: Among 360 individuals (53% men) with PWS, 16% had diabetes mellitus, 6% heart failure, 4% vein thrombosis, 2% atrial fibrillation, 2% coronary heart disease, and 1% pulmonary embolism. Individuals with PWS had an increased rate of heart failure (HR: 23.85; 95% CI: 14.09–40.38), diabetes mellitus (HR: 17.49; 95% CI: 12.87–23.74), vein thrombosis (HR: 10.44; 95% CI: 5.69–19.13), pulmonary embolism (HR: 5.77; 95% CI: 2.27–14.67), atrial fibrillation (HR: 5.19; 95% CI: 2.48–10.86), and coronary heart disease (HR: 3.46; 95% CI: 1.50–7.97) compared to non-PWS individuals. Somatotropin was prescribed in 63%, antidiabetics in 18%, and thyroid hormones in 16% of the PWS individuals (<1%, 2%, and 3%, respectively, in non-PWS individuals). The rate of mortality was fifteen times higher in PWS than in non-PWS, with a mean age at death of 42 years. Conclusions: The rates of diabetes mellitus and cardiovascular comorbidities were higher in individuals with PWS. As expected, the prescription of somatotropin was high, but the endocrine prescription pattern also reflected the high prevalence of diabetes mellitus and thyroid illness. Although the mean age at death was older than previously reported, a higher awareness and intensified efforts to avoid obesity, as well as the prevention and early treatment of cardiovascular and endocrine comorbidity, are crucial aims in the care of people with PWS.
Evaluation of the Prevalence of Sleep Disorders and Their Association with Stroke: A Hospital-Based Retrospective Study
Authors: Alabdali Majed Mohammad , Alrasheed Abdulrahim Saleh , Alsamih Faynan Sultan , Almohaish Reenad Fahad , Al Hadad Jumana Nasser , AlMohish Noor Mohammad , AlGhamdi Omar Ali , Alabdulaali Suliman Khalid , Alabdi Zainab Ibrahim
Published: February 2025

Background: Sleep disturbances are prevalent among stroke survivors, significantly impacting their recovery and quality of life. This study aimed to evaluate the prevalence of sleep disorders, sleep quality, risk of obstructive sleep apnea (OSA), and daytime sleepiness among stroke survivors and to identify potential associations with clinical and demographic factors. Materials and Methods: A retrospective observational study analyzed adult stroke survivors (aged ≥ 18 years) attending neurology clinics at our institution from November 2022 to November 2024. The primary outcome measures included overall sleep quality, sleep apnea and daytime sleepiness assessment. Data were collected using validated Arabic versions of the Pittsburgh Sleep Quality Index (PSQI), STOP-Bang Questionnaire, and Epworth Sleepiness Scale (ESS). Statistical analyses, including Chi-square tests and t-tests, were performed using SPSS version 30.1. Results: A total of 100 stroke survivors, mostly aged 40–60 years, were recruited in our study. The prevalence of sleep disorders was 60.0%, with poor sleep quality reflected by a mean global PSQI score of 9.13 ± 14.40. Additionally, 19.0% were at high risk of OSA, and 24.0% experienced abnormal daytime sleepiness. While no statistically significant associations were found between sleep disorders and clinical or demographic factors, trends indicated higher sleep disorder prevalence in those with hemorrhagic stroke and high-risk OSA profiles. Conclusions: Our study highlights a high prevalence of sleep disorders among stroke survivors, emphasizing the need for regular sleep assessments. Future studies should explore objective assessments and larger sample sizes to validate these findings and to assess their potential implication in stroke recovery and quality of life.
Impact of Prior Selinexor Exposure on Outcomes of Chimeric Antigen Receptor T-Cell Therapy for Relapsed/Refractory Multiple Myeloma: An Exploratory Analysis
Authors: Costa Bruno Almeida , Dima Danai , Mark Tomer , Sadek Norah Layla , Ijioma Stephen , Ray David , Goel Utkarsh , Dranitsaris George , Sheng Tianxiang , Moshier Erin , Mouhieddine Tarek H. , Khouri Jack , Rossi Adriana
Published: February 2025

Background/Objectives: Chimeric antigen receptor T-cell therapy (CAR-T) has become a key treatment option for relapsed/refractory multiple myeloma (RRMM), but factors impairing T-cell fitness may diminish efficacy. Our exploratory analysis aimed to evaluate the impact of prior treatment with a selinexor-containing regimen on CAR-T outcomes for RRMM patients. Methods: Data for this retrospective cohort study were sourced from electronic medical records at two US academic centers. Kaplan–Meier estimates assessed duration of response (DOR), progression-free survival (PFS), and overall survival (OS), reported as medians with interquartile ranges (IQRs). Cox proportional hazards regression analyzed factors potentially associated with PFS and OS, reported as hazard ratios (HRs) with 95% confidence intervals (CIs). Results: Among 45 patients exposed to selinexor before undergoing BCMA-directed CAR-T, median therapy line numbers for selinexor use and CAR-T were 7 and 9, respectively, with 24.4% receiving selinexor as part of bridging. At median follow-up of 68 months, median PFS and OS post CAR-T were 8.0 (IQR 3.1–39.5) and 35.9 (IQR 14.2–NR) months, respectively. Overall response rate to CAR-T was 89%, with a median DOR of 8.1 months (IQR 2.9–39.0). In our multivariable model, patients who received a selinexor-based regimen in the line of therapy preceding CAR-T showed a trend toward reduced risk of death (HR = 0.08; 95% CI 0.02–0.46) and/or disease progression (HR = 0.40; 95% CI 0.14–1.09). Conclusions: Prior selinexor exposure does not appear to compromise CAR-T outcomes in heavily pretreated RRMM, suggesting potential T-cell sparing. Our findings warrant larger, prospective studies to determine whether preemptive selinexor treatment can optimize CAR-T efficacy.
18F-FDG PET/CT in the Preoperative Diagnostic and Staging of Lung Cancer and as a Predictor of Lymph Node Involvement
Authors: Viohl Nathalie , Steinert Matthias , Freesmeyer Martin , Kühnel Christian , Drescher Robert
Published: February 2025

Background/Objectives: The aim of this study was to evaluate the efficacy and accuracy of PET imaging and performance in defining the preoperative TNM classification, especially the intrathoracic lymph node staging, of patients with lung cancer. Methods: A retrospective, single-institution study of consecutive patients with surgical therapy of lung cancer that were undergoing preoperative PET/CT scanning at the same center was conducted. A total of 104 patients were included. All patients underwent surgical evaluation with mediastinal and hilar lymph node sampling. Five patients with preoperative suspicion of N3 nodal status who were only tested for N2 were excluded from the observations and analyses of nodal status. Results: PET/CT staged the nodal status correctly in 85 out of 99 patients (85.9%); overstaging occurred in 7 patients (7.1%) and understaging in 7 patients (7.1%). The overall prevalence of lymph node metastases was 42.3%. When preoperative T classification was compared with postoperative histopathological T classification, 75% patients were correctly staged, 13.5% were overstaged, and 11.5% were understaged by PET/CT. In univariate analysis, lymph node involvement was significantly associated (p < 0.05) with the following primary tumor characteristics: increasing diameter (>35 mm), a maximum standardized uptake value > 9.5, and higher grading. The tumor diameter and the degree of differentiation were found to be factors influencing the SUVmax of the primary tumor as well. Conclusions: Our data show that integrated PET/CT provides high accuracy in the intrathoracic nodal staging and tumor expansion of lung cancer patients and emphasizes the continued need for surgical staging.
Quality of Life Assessment After Pelvic Prolapse Surgery With and Without Mesh: A Literature Review
Authors: Pirtea Marilena , Balint Oana , Secoșan Cristina , Costăchescu Dan , Dabîca Alexandru , Navolan Dan
Published: February 2025

Background/Objectives: The quality of life (QoL) in patients undergoing pelvic prolapse surgery is a critical area of research in urogynecology. Pelvic organ prolapse (POP) is a prevalent condition affecting a significant number of women, leading to various physical and psychological symptoms that can severely impact their quality of life. Surgical intervention aims not only to correct the anatomical defects but also to enhance the overall well-being of patients. Methods: A comprehensive literature search in the main databases was conducted for studies evaluating quality of life after surgical treatment using techniques with and without mesh. Results: A total of 35 studies met the inclusion criteria, involving a total of 4603 patients. Twenty-two distinct patient-reported outcomes (PRO) questionnaires have been identified as post-surgical QoL assessment tools. Conclusions: Quality of life is significantly improved by surgical treatment of pelvic organ prolapse. Post-surgical PRO scores do not seem to be influenced by the surgical technique used, with no significant differences between methods using mesh or not.
The Impact of the Position of the Upper Eyelid on the Profile of Epithelial Thickness and the Topography of the Cornea
Authors: Marta Kroczek , Dagmara Kudelska , Patryk Młyniuk , Bartłomiej Kałużny
Published: February 2025

Aim: To evaluate the impact of the position of the upper eyelid on the corneal epithelial thickness, anterior elevation map, and corneal aberrations in patients with unimpaired function of the eyelids. Methods: Sixty-one right eyes were included in this prospective, non-randomized study. The low-positioned eyelid group (LP group) consisted of 30 patients with a mean upper eyelid margin position at 3.45 ± 0.45 mm above the corneal apex at primary gaze. The high-positioned eyelid group (HP group) comprised 31 eyes for which the respective value was 4.56 ± 0.36 mm. The anterior elevation map, corneal aberrations, and epithelial thickness profile were obtained with eyes wide open, using a MS-39 corneal tomograph (CSO, Florence, Italy). The analysis was also performed at measurement points 0.3 mm above and 0.3 mm below the eyelid margin. Results: Significantly thinner epitheliums at 4.5 mm above the center of the cornea on the vertical meridian in the LP group were observed (p < 0.05). Higher anterior elevations and thicker epitheliums of the cornea were observed at the measurement location of 0.3 mm above the upper eyelid’s margin in the HP group (p < 0.05). A negative correlation between the height of the upper eyelid and spherical aberration was noted. Conclusions: The findings provide evidence that the positioning of the upper eyelid in individuals with normal eyelid function influences the epithelial thickness map, the anterior elevation map, and corneal spherical aberrations.
Mapping of Patellar Fracture Patterns: A Multicenter Study of 237 Patients
Authors: Lenz Julia Elisabeth , Schraag Amadeus Dominik , Plank Luis , von Rüden Christian , Alt Volker , Weber Johannes
Published: February 2025

Background/Objectives: Patellar fractures are rare but clinically significant due to their impact on knee function. These injuries vary from simple transverse to complex comminuted patterns. Computed tomography (CT) offers superior visualization compared to radiographs, enabling accurate classification and surgical planning. This study utilized CT-based fracture mapping to analyze fracture patterns and evaluate the impact of age, trauma-center level, and AO/OTA classification. Methods: This retrospective study included 237 patients diagnosed with patellar fractures who underwent CT imaging. Fractures were classified using the AO/OTA system, and fracture mapping was performed by overlaying fracture lines onto a standardized template. Statistical analysis assessed correlations between patient demographics, trauma-center level, and fracture patterns. Results: The cohort comprised 107 males and 130 females with a mean age of 56.9 ± 20.9 years. Males were significantly younger than females (49.2 vs. 63.3 years, p < 0.001). Fractures were evenly distributed between the right (46%) and left (54%) patellae. Type C fractures were the most common (54.4%), followed by Type B (29.9%) and Type A (15.6%). Trauma-center level was inversely associated with fracture severity (p < 0.001), with complex fractures more common at lower-level centers. Age was positively correlated with fracture severity (p = 0.001). Fracture mapping revealed the central patella as the most frequently affected region, with transverse fractures extending medially and laterally, sparing the upper and lower poles. Conclusions: CT imaging enhances the classification and mapping of patellar fractures, highlighting the central patella as the primary site of injury. Fracture severity correlates with age and trauma-center level. These findings support CT-based mapping as a valuable tool for improving surgical planning and treatment outcomes.
Analysis of Surgical Stabilization Results of Radial Head Fractures
Authors: Niewczas Paweł , Piekarczyk Piotr , Jacuniak Łukasz , Lewandowski Dawid , Ząbkowski Tomasz , Ciechan Kamil , Cieślik Piotr
Published: February 2025

Background/Objectives: According to the modified Mason classification, radial head fractures can be treated with open reduction and internal fixation (ORIF), radial head arthroplasty, or resection. This study by the Department of Traumatology and Orthopedics of the Military Institute of Medicine aimed to compare the clinical and radiological outcomes of fractures treated with ORIF. Methods: This retrospective study analyzed 55 patients with radial head fractures treated between April 2020 and February 2023. Fractures were classified using Mason system as 15 type II, 26 type III, and 14 type IV. Clinical outcomes were evaluated using the VAS for pain, Broberg–Morrey scale, and the DASH questionnaire, alongside assessments of range of motion, grip strength, and elbow stability. Follow-up radiographs examined bone union, bone fragments displacement, degenerative changes and periarticular ossification. Results: The mean follow-up period was 21.0 ± 10.2 months. There were no statistically significant differences in VAS scale results for Mason types II, III, and IV fractures (4.0 vs. 6.0 vs. 5.0, respectively; p = 0.825), the Broberg–Morrey scale (82.0 ± 15.2 vs. 80.9 ± 15.5 vs. 84.2 ± 15.1, respectively; p = 0.845), or the DASH questionnaire (10.0 vs. 11.7 vs. 17.5, respectively; p = 0.937). Mean extension deficit and supination angles were at the limit of statistical significance (p = 0.076 and p = 0.051). No cases of lateral instability were observed, whereas medial instability was seen in only one case. Bone union was observed in 97.5% of cases, with elbow joint osteoarthritis and periarticular ossification in 15.0% and 45.0% of cases, respectively. Conclusions: Mason type II, III and IV radial head fractures treated with open reduction and internal fixation showed good functional and radiological outcomes with rare complications, including degenerative changes, periarticular ossifications and nonunion.
Unraveling the Role of PET in Cervical Cancer: Review of Current Applications and Future Horizons
Authors: Yadav Divya , O’Dwyer Elisabeth , Agee Matthew , Dutruel Silvina P. , Mahajan Sonia , Huicochea Castellanos Sandra
Published: February 2025

FDG PET/CT provides complementary metabolic information with greater sensitivity and specificity than conventional imaging modalities for evaluating local recurrence, nodal, and distant metastases in patients with cervical cancer. PET/CT can also be used in radiation treatment planning, which is the mainstay of treatment. With the implementation of various oncological guidelines, FDG PET/CT has been utilized more frequently in patient management and prognostication. Newer PET tracers targeting the tumor microenvironment offer valuable biologic insights to elucidate the mechanism of treatment resistance and tumor aggressiveness and identify the high-risk patients. Artificial intelligence and machine learning approaches have been utilized more recently in metastatic disease detection, response assessment, and prognostication of cervical cancer.
Using Cognitive Diagnostic Models to Evaluate the Two-Process Theory of Matrix Reasoning
Authors: Preuß Julian , Preckel Franzis
Published: February 2025

Figural matrices are widely used to measure reasoning ability. According to the two-process model of figural matrix reasoning, task performance relies on correspondence finding (linked to induction ability) and goal management (linked to working memory). Cognitive theory suggests that item characteristics (i.e., change rules and design principles of figural elements) are related to the two solution processes and impact item difficulties in a multiplicative, interactive manner. This study tested the multiplicative effect hypothesis by comparing two cognitive diagnostic models using additive and multiplicative effect estimations. A 26-item figural matrix test was administered to 633 high-ability individuals across paper-and-pencil and computer formats. The linear logistic test model (LLTM) and least square distance method (LSDM) were applied to Rasch and 2PL item parameters. Contrary to the multiplicative effect hypothesis, the additive LLTM model showed better item parameter reconstruction than the LSDM that includes multiplicative effects. These results suggest that change rules and design principles may independently contribute to the difficulty of figural matrices. Correspondence-finding demands may primarily arise from design principles, while change rules may primarily contribute to difficulty through goal management demands based on their number and complexity. The findings highlight the need to consider item components related to the phenomenological representation of figural elements when explaining solution processes of figural matrices. Implications for cognitive theory and item construction are discussed.
Target Ship Recognition and Tracking with Data Fusion Based on Bi-YOLO and OC-SORT Algorithms for Enhancing Ship Navigation Assistance
Authors: Chen Shuai , Gao Miao , Shi Peiru , Zeng Xi , Zhang Anmin
Published: February 2025

With the ever-increasing volume of maritime traffic, the risks of ship navigation are becoming more significant, making the use of advanced multi-source perception strategies and AI technologies indispensable for obtaining information about ship navigation status. In this paper, first, the ship tracking system was optimized using the Bi-YOLO network based on the C2f_BiFormer module and the OC-SORT algorithms. Second, to extract the visual trajectory of the target ship without a reference object, an absolute position estimation method based on binocular stereo vision attitude information was proposed. Then, a perception data fusion framework based on ship spatio-temporal trajectory features (ST-TF) was proposed to match GPS-based ship information with corresponding visual target information. Finally, AR technology was integrated to fuse multi-source perceptual information into the real-world navigation view. Experimental results demonstrate that the proposed method achieves a mAP0.5:0.95 of 79.6% under challenging scenarios such as low resolution, noise interference, and low-light conditions. Moreover, in the presence of the nonlinear motion of the own ship, the average relative position error of target ship visual measurements is maintained below 8%, achieving accurate absolute position estimation without reference objects. Compared to existing navigation assistance, the AR-based navigation assistance system, which utilizes ship ST-TF-based perception data fusion mechanism, enhances ship traffic situational awareness and provides reliable decision-making support to further ensure the safety of ship navigation.
Advanced Studies in Breakwaters and Coastal Protection
Authors: Reis Maria Teresa , Santos João Alfredo , Fazeres-Ferradosa Tiago
Published: February 2025

Globally, mean sea level rise has been accelerating, populations living in coastal zones have been gradually growing, including in low-lying coasts, and the demands for deeper harbors have been quickly increasing to accommodate the future deeper-draft vessels that are being bought online by shipping companies [...]