bluuebunny/crossref_metadata_2025 huggingface dataset:
Structure-affinity relationship of dietary anthocyanin–HSA interaction
Authors: Zhang Jiaojiao , Zuo Benrong , Poklar Ulrih Nataša , Sengupta Pradeep K. , Zheng Xiaodong , Xiao Jianbo
Published: October 2017

BACKGROUND: Dietary anthocyanins are plant pigments which occur with different chemical structures, being widely present in fruits and in many vegetables, are claimed to be beneficial for human health. The bioavailability of anthocyanins is the key factor influencing their health benefits. OBJECTIVE: Herein, the molecular structure-affinity relationship of anthocyanin–human serum albumin interaction was investigated. METHODS: Fluorescence quenching method was applied to determine the binding affinities of anthocyanins for human serum albumin. RESULTS: Demethylation of the methoxyl groups in anthocyanins enhanced the binding affinities. The number and position of the hydroxyl groups on ring [B] affect the affinities of anthocyanins for human serum albumin. The glycosylation of hydroxyl groups on ring [C] enhanced their binding affinities for human serum albumin. CONCLUSIONS: Anthocyanidins and anthocyanins, show different characteristics for their binding to human serum albumin when the methoxyl groups on the ring B are demethylated or hydroxylated.
A novel low-cost approach for navigated transcranial magnetic stimulation
Authors: Rodseth Jakob , Washabaugh Edward P. , Krishnan Chandramouli
Published: October 2017

Background:

Transcranial magnetic stimulation (TMS) is commonly used for assessing or modulating brain excitability. However, the credibility of TMS outcomes depends on accurate and reliable coil placement during stimulation. Navigated TMS systems can address this issue, but these systems are expensive for routine use in clinical and research environments.

Objective:

The purpose of this study was to provide a high-quality open source framework for navigated TMS and test its reliability and accuracy using standard TMS procedures.

Methods:

A navigated TMS system was created using a low-cost 3D camera system (OptiTrack Trio), which communicates with our free and open source software environment programmed using the Unity 3D gaming engine. The environment is user friendly and has functions to allow for a variety of stimulation procedures (e.g., head and coil co-registration, multiple hotspot/grid tracking, intuitive matching, and data logging). The system was then validated using a static mockup of a TMS session. The clinical utility was also evaluated by assessing the repeatability and operator accuracy when collecting motor evoked potential (MEP) data from human subjects.

Results:

The system was highly reliable and improved coil placement accuracy (position error = 1.2 mm and orientation error = 0.3°) as well as the quality and consistency (ICC >0.95) of MEPs recorded during TMS.

Conclusion:

These results indicate that the proposed system is a viable tool for reliable coil placement during TMS procedures, and can improve accuracy in locating the coil over a desired hotspot both within and between sessions.
Smooth leader or sharp follower? Playing the mirror game with a robot
Authors: Kashi Shir , Levy-Tzedek Shelly
Published: October 2017

Background:

The increasing number of opportunities for human-robot interactions in various settings, from industry through home use to rehabilitation, creates a need to understand how to best personalize human-robot interactions to fit both the user and the task at hand. In the current experiment, we explored a human-robot collaborative task of joint movement, in the context of an interactive game.

Objective:

We set out to test people’s preferences when interacting with a robotic arm, playing a leader-follower imitation game (the mirror game).

Methods:

Twenty two young participants played the mirror game with the robotic arm, where one player (person or robot) followed the movements of the other. Each partner (person and robot) was leading part of the time, and following part of the time. When the robotic arm was leading the joint movement, it performed movements that were either sharp or smooth, which participants were later asked to rate.

Results:

The greatest preference was given to smooth movements. Half of the participants preferred to lead, and half preferred to follow. Importantly, we found that the movements of the robotic arm primed the subsequent movements performed by the participants.

Conclusion:

The priming effect by the robot on the movements of the human should be considered when designing interactions with robots. Our results demonstrate individual differences in preferences regarding the role of the human and the joint motion path of the robot and the human when performing the mirror game collaborative task, and highlight the importance of personalized human-robot interactions.
Creating a multi-center rare disease consortium – the Consortium of Eosinophilic Gastrointestinal Disease Researchers (CEGIR)
Authors: Cheng Katherine , Gupta Sandeep K. , Kantor Susanna , Kuhl Jonathan T. , Aceves Seema S. , Bonis Peter A. , Capocelli Kelley E. , Carpenter Christina , Chehade Mirna , Collins Margaret H. , Dellon Evan S. , Falk Gary W. , Gopal-Srivastava Rashmi , Gonsalves Nirmala , Hirano Ikuo , King Eileen C. , Leung John , Krischer Jeffrey P. , Mukkada Vincent A. , Schoepfer Alain , Spergel Jonathan M. , Straumann Alex , Yang Guang-Yu , Furuta Glenn T. , Rothenberg Marc E.
Published: October 2017

Eosinophilic gastrointestinal disorders (EGIDs) affect various segments of the gastrointestinal tract. Since these disorders are rare, collaboration is essential to enroll subjects in clinical studies and study the broader population. The Rare Diseases Clinical Research Network (RDCRN), a program of the National Center for Advancing Translational Sciences (NCATS), funded the Consortium of Eosinophilic Gastrointestinal Disease Researchers (CEGIR) in 2014 to advance the field of EGIDs. CEGIR facilitates collaboration among various centers, subspecialties, patients, professional organizations and patient-advocacy groups and includes 14 clinical sites. It has successfully initiated two large multi-center clinical studies looking to refine EGID diagnoses and management. Several pilot studies are underway that focus on various aspects of EGIDs including novel therapeutic interventions, diagnostic and monitoring methods, and the role of the microbiome in pathogenesis. CEGIR currently nurtures five physician-scholars through a career training development program and has published more than 40 manuscripts since its inception. This review focuses on CEGIR’s operating model and progress and how it facilitates a framework for exchange of ideas and stimulates research and innovation. This consortium provides a model for progress on other potential clinical areas.
Image artifacts and noise reduction algorithm for cone-beam computed tomography with low-signal projections
Authors: Yang Fu-Qiang , Zhang Ding-Hua , Huang Kui-Dong , Yang Ya-Fei , Liao Jin-Ming
Published: October 2017

This study aims to investigate and test a new image reconstruction algorithm applying to the low-signal projections to generate high quality images by reducing the artifacts and noise in the cone-beam computed tomography (CBCT). For the low-signal and noisy projections, a multiple sampling method is first utilized in projection domain to suppress environmental noise, which guarantees the accuracy of the data for reconstruction, simultaneously. Next, a fuzzy entropy based method with block matching 3D (BM3D) filtering algorithm is employed to improve the image quality to reduce artifacts and noise in image domain. Then, simulation studies on polychromatic spectrum were performed to evaluate the performance of the proposed new algorithm. Study results demonstrated significant improvement in the signal-to-noise ratios (SNRs) and contrast-to-noise ratios (CNRs) of the images reconstructed using the new algorithm. SNRs and CNRs of the new images were averagely 40% and 20% higher than those of the previous images reconstructed using the traditional algorithms, respectively. As a result, since the new image reconstruction algorithm effectively reduced the artifacts and noise, and produced images with better contour and grayscale distribution, it has the potential to improve image quality using the original CBCT data with the low and missing signals.
Geometric artifacts reduction for cone-beam CT via L0-norm minimization without dedicated phantoms
Authors: Gong Changcheng , Cai Yufang , Zeng Li
Published: October 2017

For cone-beam computed tomography (CBCT), transversal shifts of the rotation center exist inevitably, which will result in geometric artifacts in CT images. In this work, we propose a novel geometric calibration method for CBCT, which can also be used in micro-CT. The symmetry property of the sinogram is used for the first calibration, and then L0-norm of the gradient image from the reconstructed image is used as the cost function to be minimized for the second calibration. An iterative search method is adopted to pursue the local minimum of the L0-norm minimization problem. The transversal shift value is updated with affirmatory step size within a search range determined by the first calibration. In addition, graphic processing unit (GPU)-based FDK algorithm and acceleration techniques are designed to accelerate the calibration process of the presented new method. In simulation experiments, the mean absolute difference (MAD) and the standard deviation (SD) of the transversal shift value were less than 0.2 pixels between the noise-free and noisy projection images, which indicated highly accurate calibration applying the new calibration method. In real data experiments, the smaller entropies of the corrected images also indicated that higher resolution image was acquired using the corrected projection data and the textures were well protected. Study results also support the feasibility of applying the proposed method to other imaging modalities.
Effects of Early Development of Hyponatremia on In-hospital Outcomes in Acute ST- Elevation Myocardial Infarction
Authors: Islam Mirza Md Nazrul , Kader Md Abdul , Khan HI Lutfor Rahman , Khan Mahmood Hasan
Published: October 2017

Background: Coronary heart disease is a major cause of death and is a global health problem and reaching epidemic in both developed and as well as in developing countries. In patients with acute myocardial infarction baroreceptor mediated hormonal activation has a prognostic value. Clinical importance of hyponatraemia in ST-elevation acute myocardial infarction has not been fully understood. Hyponatremia which developed in early phase of AMI has been recently advocated as an important prognostic factor in several studies.Objectives: The aim of the study was to investigate the impact of hyponatremia on in-hospital outcomes in patients with ST-elevation acute myocardial Infarction treated by thrombolysis.Methods: Hyponartaemia was defined as a sodium concentration <135 mmol/L. Hyponatremia which developed at 72 hours after admission was defined as early developed hyponatraemia. This study is a prospective observational study. A purposive sampling technique was used to select the sample. In this study data on 100 patients with ST-elevation acute myocardial infarction were analyzed. This study was done in the department of Cardiology Mymensingh Medical College Hospital from July 2014 to June 2015. Patients admitted in the department of Cardiology MMCH with acute STelevation myocardial infarction and treated with thrombolysis were evaluated for serum sodium level at admission and at 72 hours after admission. Total 100 patients were studied. Fifty patients with hyponatraemia were Group-I and fifty patients with normal sodium level were Group-II. Then the in-hospital outcome variables were analyzed.Results: Among the study population 85% were male and 15% were female. Age range was 25 years to 74 years. Mean age was 52.88±11.88. Risk factors such as smoking, hypertension, diabetes mellitus, dyslipidemia and F/H of CAD were evaluated. Highest percentage of study population had hypertension (52%) followed by dyslipidemia (49%), smoker (46%), diabetes mellitus (39%) and F/H of CAD had (24%) of study population. There were five outcome variables such as heart failure, cardiogenic shock, arrhythmia, duration of hospital stay and death. Total 12 patients died. 10 patients died in Group-I and 2 patients died in Group-II. Among the outcome variables death, heart failure and hospital stay was more in Group-I and was statistically significant.Conclusion: Early developed hyponatremia in patients with ST-elevation acute myocardial infarction was an independent predictor of prognosis. Heart failure, duration of hospital stay and death was more in hyponatraemic patients and Prognosis worsen with increasing severity of hyponatremia. Plasma sodium level may serve as a simple marker to identify patient at high risk.Bangladesh Heart Journal 2017; 32(1) : 29-35
Fast Reconfigurable SOA-Based Wavelength Conversion of Advanced Modulation Format Data
Authors: Lin Yi , P. Anthur Aravind , P. Ó Dúill Sean , Liu Fan , Yu Yonglin , P. Barry Liam
Published: October 2017

We theoretically analyze the phase noise transfer issue between the pump and the wavelength-converted idler for a nondegenerate four-wave mixing (FWM) scheme, as well as study the vector theory in nonlinear semiconductor optical amplifiers (SOAs), in order to design a polarization-insensitive wavelength conversion system employing dual co-polarized pumps. A tunable sampled grating distributed Bragg reflector (SG-DBR) pump laser has been utilized to enable fast wavelength conversion in the sub-microsecond timescale. By using the detailed characterization of the SGDBR laser, we discuss the phase noise performance of the SGDBR laser. Finally, we present a reconfigurable SOA-based all-optical wavelength converter using the fast switching SGDBR tunable laser as one of the pump sources and experimentally study the wavelength conversion of the single polarization quadrature phase shift keying (QPSK) and polarization multiplexed (Pol-Mux) QPSK signals at 12.5-Gbaud. A wide tuning range (>10 nm) and less than 50 ns and 160 ns reconfiguration time have been achieved for the wavelength conversion system for QPSK and PM-QPSK signals, respectively. The performance under the switching environment after the required reconfiguration time is the same as the static case when the wavelengths are fixed.
A Perspective on the Experimental Techniques for Studying Lamins
Authors: Pecorari Ilaria , Borin Daniele , Sbaizero Orfeo
Published: October 2017

Lamins are type V intermediate filaments that collectively form a meshwork underneath the inner nuclear membrane, called nuclear lamina. Furthermore, they are also present in the nucleoplasm. Lamins are experiencing a growing interest, since a wide range of diseases are induced by mutations in the gene coding for A-type lamins, globally known as laminopathies. Moreover, it has been demonstrated that lamins are involved in other pathological conditions, like cancer. The role of lamins has been studied from several perspectives, exploiting different techniques and procedures. This multidisciplinary approach has contributed to resolving the unique features of lamins and has provided a thorough insight in their role in living organisms. Yet, there are still many unanswered questions, which constantly generate research in the field. The present work is aimed to review some interesting experimental techniques performed so far to study lamins. Scientists can take advantage of this collection for their novel investigations, being aware of the already pursued and consolidated methodologies. Hopefully, advances in these research directions will provide insights to achieve better diagnostic procedures and effective therapeutic options.
Comparative Study of Monsoon Rainfall Variability over India and the Odisha State
Authors: Gouda K , Sahoo Sanjeeb , Samantray Payoshni , Shivappa Himesh
Published: October 2017

Indian summer monsoon (ISM) plays an important role in the weather and climate system over India. The rainfall during monsoon season controls many sectors from agriculture, food, energy, and water, to the management of disasters. Being a coastal province on the eastern side of India, Odisha is one of the most important states affected by the monsoon rainfall and associated hydro-meteorological systems. The variability of monsoon rainfall is highly unpredictable at multiple scales both in space and time. In this study, the monsoon variability over the state of Odisha is studied using the daily gridded rainfall data from India Meteorological Department (IMD). A comparative analysis of the behaviour of monsoon rainfall at a larger scale (India), regional scale (Odisha), and sub-regional scale (zones of Odisha) is carried out in terms of the seasonal cycle of monsoon rainfall and its interannual variability. It is seen that there is no synchronization in the seasonal monsoon category (normal/excess/deficit) when analysed over large (India) and regional (Odisha) scales. The impact of El Niño, La Niña, and the Indian Ocean Dipole (IOD) on the monsoon rainfall at both scales (large scale and regional scale) is analysed and compared. The results show that the impact is much more for rainfall over India, but it has no such relation with the rainfall over Odisha. It is also observed that there is a positive (negative) relation of the IOD with the seasonal monsoon rainfall variability over Odisha (India). The correlation between the IAV of monsoon rainfall between the large scale and regional scale was found to be 0.46 with a phase synchronization of 63%. IAV on a sub-regional scale is also presented.
Analysis of the Effect of Channel Leakage on Design, Characterization and Modelling of a High Voltage Pseudo-Floating Gate Sensor-Front-End
Authors: Marchetti Luca , Berg Yngvar , Azadmehr Mehdi
Published: October 2017

In this paper, we analyze the effects of channel leakage on the design, modelling and characterization of a high voltage pseudo-floating gate amplifier (PFGA) used as sensor front-end. Leakages are known as a major challenge in new modern CMOS technologies, which are used to bias the PFGA, and consequently affect the behavior of the amplifier. As high voltages are desired for actuation of many types of resonating sensors, especially in ultrasound applications, PFGA implemented in high voltage and low leakage technologies, such as older CMOS fabrication processes or power MOSFET can be the only option. The challenge with these technologies used to implement the PFGA is that the leakages are very low, which affect the biasing of the floating gate. However, the numerous advantages of this type of amplifier, implemented with modern fabrication processes, such as high flexibility, compactness, low power consumption , etc. encouraged the authors to research about this topic. This work provides analysis of the working principle and the design rules for this amplifier, emphasizing the major differences between PFGA implemented in low leakage and high leakage technologies. Static and dynamic analysis, input offset and non-linearity of the PFGA are the main topics of this article. Three different design approaches are presented in this paper, in order to provide a more general design procedure and offset compensation for any low leakage PFGA. The amplifier has been simulated in AMS- 0 . 35 μ m CMOS models for supply voltages of 5 V and 10 V. Two prototypes have been realized to verify the validity of the modelling and the simulation results. Both devices have been realized by using discrete components and mounted on a printed circuit board. In this work, MOSFETs are realized by using commercial IC CD4007UB and 2N7000. Measurement results of the first prototype proved that the implementation of a low leakage PFGA is possible after that the input offset of the amplifier has been compensated. Measurement results of the second prototype have been used to characterize the low leakage PFGA, extracting the best performances from this amplifier, realized with less components and providing a more compact device. Finally, design rules have been summarized in order to implement this amplifier, which enjoys compactness and a relative low power dissipation.
Finite Control Set Model Predictive Control for a Three-Phase Shunt Active Power Filter with a Kalman Filter-Based Estimation
Authors: Guzmán Ramon , García de Vicuña Luís , Castilla Miguel , Miret Jaume , Camacho Antonio
Published: October 2017

In this paper, the finite control set model predictive control is combined with the vector operation technique to be applied in the control of a three-phase active power filter. Typically, in the finite control set technique applied to three-phase power converters, eight different vectors are considered in order to obtain the optimum control signal by minimizing a cost function. On the other hand, the vector operation technique is based on dividing the grid voltage period into six different regions. The main advantage of combining both techniques is that for each region the number of possible voltage vectors to be considered can be reduced to a half, thus reducing the computational load employed by the control algorithm. Besides, in each region, only two phase-legs are switching at high frequency while the remaining phase-leg is maintained to a constant dc-voltage value during this interval. Accordingly, a reduction of the switching losses is obtained. Unlike the typical model predictive control methods which make use of the discrete differential equations of the converter, this method considers a Kalman filter in order to improve the behavior of the closed-loop system in noisy environments. Selected experimental results are exposed in order the demonstrate the validity of the control proposal.
Chemical Characteristics and NaCl Component Behavior of Biochar Derived from the Salty Food Waste by Water Flushing
Authors: Lee Ye-Eun , Jo Jun-Ho , Kim I-Tae , Yoo Yeong-Seok
Published: October 2017

Biochar is the product of the pyrolysis of organic materials in a reduced state. In recent years, biochar has received attention due to its applicability to organic waste management, thereby leading to active research on biochar. However, there have been few studies using food waste. In particular, the most significant difference between food waste and other organic waste is the high salinity of food waste. Therefore, in this paper, we compare the chemical characteristics of biochar produced using food waste containing low- and high-concentration salt and biochar flushed with water to remove the concentrated salt. In addition, we clarify the salt component behavior of biochar. Peak analysis of XRD confirms that it is difficult to find salt crystals in flushed char since salt remains in the form of crystals when salty food waste is pyrolyzed washed away after water flushing. In addition, the Cl content significantly decreased to 1–2% after flushing, similar to that of Cl content in the standard, non-salted food waste char. On the other hand, a significant amount of Na was found in pyrolyzed char even after flushing resulting from a phenomenon in which salt is dissolved in water while flushing and Na ions are adsorbed. FT-IR analysis showed that salt in waste affects the binding of aromatic carbons to compounds in the pyrolysis process. The NMR spectroscopy demonstrated that the aromatic carbon content, which indicates the stability of biochar, is not influenced by the salt content and increases with increasing pyrolysis temperature.
Verification of the Performance of a Vertical Ground Heat Exchanger Applied to a Test House in Melbourne, Australia
Authors: Ooi Koon , Noguchi Masa
Published: October 2017

The ground heat exchanger is traditionally used as a heat source or sink for the heat pump that raises the temperature of water to about 50 °C to heat houses. However, in winter, the heating thermostat (temperature at which heating begins) in the Australian Nationwide House Energy Rating Scheme (NatHERS) is only 20 °C during daytime and 15 °C at night. In South-East Melbourne, the temperature at the bottom of a 50-meter-deep borehole has been recorded with an Emerson™ recorder at 17 °C. Melbourne has an annual average temperature of 15 °C, so the ground temperature increases by 2 °C per 50-m depth. A linear projection gives 23 °C at 200-m of depth, and as the average undisturbed temperature of the ground for a 400-m-deep vertical ground heat exchanger (VGHE). This study, by simulation and experimentation, aims to verify that the circulation of water in the VGHE’s U-tube to low-temperature radiators (LTRs) could heat a house to thermal comfort. A literature review is included in the introduction. A simulation, using a model of a 60-m2 experimental house, shows that the daytime circulation of water in this VGHE/LTR-on-opposite-walls system during the 8-month cold half of the year, heats the indoors to NatHERS settings. Simulation for the cold half shows that this VGHE-LTR system could cool the indoors. Instead, a fan creating a cooling sensation of up to 4 °C is used so that the VGHE is available for the regeneration of heat extracted from the ground during the cold portion. Simulations for this hot portion show that a 3.4-m2 flat plate solar collector can collect more than twice the heat extracted from the ground in the cold portion. Thus, it can thus replenish the ground heat extracted for houses double the size of this 60-m2 experimental house. Therefore, ground heat is sustainable for family-size homes. Since no heat pump is used, the cost of VGHE-LTR systems could be comparable to systems using the ground source heat pump. Water circulation pumps and fans require low power that can be supplied by photovoltaic thermal (PVT). The EnergyPlus™ v8.7 object modeling the PVT requires user-defined efficiencies, so a PVT will be tested in the experimental house.
Sociology in Global Environmental Governance? Neoliberalism, Protectionism and the Methyl Bromide Controversy in the Montreal Protocol
Authors: Gareau Brian
Published: October 2017

Sociological studies of global agriculture need to pay close attention to the protectionist aspects of neoliberalism at the global scale of environmental governance. With agri-food studies in the social sciences broadening interrogations of the impact of neoliberalism on agri-food systems and their alternatives, investigating global environmental governance (GEG) will help reveal its impacts on the global environment, global science/knowledge, and the potential emergence of ecologically sensible alternatives. It is argued here that as agri-food studies of neoliberalism sharpen the focus on these dimensions the widespread consequences of protectionism of US agri-industry in GEG will become better understood, and the solutions more readily identifiable. This paper illustrates how the delayed phase out of the toxic substance methyl bromide in the Montreal Protocol exemplifies the degree to which the US agri-industry may be protected at the global scale of environmental governance, thus prolonging the transition to ozone-friendly alternatives. Additionally, it is clear that protectionism has had a significant impact on the dissemination and interpretation of science/knowledge of methyl bromide and its alternatives. Revealing the role that protectionism plays more broadly in the agriculture/environmental governance interface, and its oftentimes negative impacts on science and potential alternatives, can shed light on how protectionism can be made to serve ends that are at odds with environmental protection.
Influence of Container Type and Growth Medium on Seedling Growth and Root Morphology of Cyclocarya paliurus during Nursery Culture
Authors: Tian Ning , Fang Shengzuo , Yang Wanxia , Shang Xulan , Fu Xiangxiang
Published: October 2017

As a multiple function tree species, Cyclocarya paliurus (Batal) Iljinskaja is mainly planted and managed for timber production and medical use. To improve the seed use efficiency and outplanting performance of C. paliurus, the effects of container types and growth medium on the seedling growth and root morphology of C. paliurus were investigated by using a completely randomized block experimental design with a 4 × 3 factorial arrangement during nursery culture. Both container type and growth medium significantly affected the growth, biomass, and root morphological indexes of C. paliurus seedlings, but container size had a greater effect on the seedling quality of C. paliurus than the growth medium formula. The root-collar diameter and height of the seedlings were positively and significantly correlated with the biomass variables and root morphological variables, and could be considered essential attributes for evaluating seedling quality. Based on the results from this study, the management regime used here in C. paliurus seedling production is suggested to ensure good quality seedling delivery. Our study provides not only valuable insights into the container seedling culture of C. paliurus, it also enables nursery managers to optimize seedling production.
Multiscale Polymer Composites: A Review of the Interlaminar Fracture Toughness Improvement
Authors: Dikshit Vishwesh , Bhudolia Somen , Joshi Sunil
Published: October 2017

Composite materials are prone to delamination as they are weaker in the thickness direction. Carbon nanotubes (CNTs) are introduced as a multiscale reinforcement into the fiber reinforced polymer composites to suppress the delamination phenomenon. This review paper presents the detailed progress made by the scientific and research community to-date in improving the Mode I and Mode II interlaminar fracture toughness (ILFT) by various methodologies including the effect of multiscale reinforcement. Methods of measuring the Mode I and Mode II fracture toughness of the composites along with the solutions to improve them are presented. The use of different methodologies and approaches along with their performance in enhancing the fracture toughness of the composites is summarized. The current state of polymer-fiber-nanotube composites and their future perspective are also deliberated.
Analysis of the Impact of Known SPINK1 Missense Variants on Pre-mRNA Splicing and/or mRNA Stability in a Full-Length Gene Assay
Authors: Wu Hao , Boulling Arnaud , Cooper David , Li Zhao-Shen , Liao Zhuan , Férec Claude , Chen Jian-Min
Published: October 2017

It is increasingly appreciated that missense variants may not only alter protein structure and function but may also influence pre-mRNA splicing and/or mRNA stability. Here we explore this issue in the context of currently known SPINK1 missense variants using a full-length gene assay. We demonstrated that 4 (17%) out of 24 variants tested significantly reduced pre-mRNA splicing and/or stability as compared with the wild-type. However, since the strongest effect observed was a 23% reduction from normal, the contribution of SPINK1 missense variants to the clinical phenotype through an impact on mRNA processing alone may be relatively minor compared with their effects in relation to protein structure/function.
Geometric Analysis on Stone Façades with Terrestrial Laser Scanner Technology
Authors: Corso Juan , Roca Josep , Buill Felipe
Published: October 2017

This article presents a methodology to process information from a Terrestrial Laser Scanner (TLS) from three dimensions (3D) to two dimensions (2D), and to two dimensions with a color value (2.5D), as a tool to document and analyze heritage buildings. Principally focused on the loss of material in stone, this study aims at creating an evaluation method for loss control, taking into account the state of conservation of a building in terms of restoration, from studying the pathologies, to their identification and delimitation. A case study on the Cathedral of the Seu Vella de Lleida was completed, examining the details of the stone surfaces. This cathedral was affected by military use, periods of abandonment, and periodic restorations.
Single Silver Nanoparticle Instillation Induced Early and Persisting Moderate Cortical Damage in Rat Kidneys
Authors: Roda Elisa , Barni Sergio , Milzani Aldo , Dalle-Donne Isabella , Colombo Graziano , Coccini Teresa
Published: October 2017

The potential toxic effects of silver nanoparticles (AgNPs), administered by a single intratracheal instillation (i.t), was assessed in a rat model using commercial physico-chemical characterized nanosilver. Histopathological changes, overall toxic response and oxidative stress (kidney and plasma protein carbonylation), paralleled by ultrastructural observations (TEM), were evaluated to examine renal responses 7 and 28 days after i.t. application of a low AgNP dose (50 µg/rat), compared to an equivalent dose of ionic silver (7 µg AgNO3/rat). The AgNPs caused moderate renal histopathological and ultrastructural alteration, in a region-specific manner, being the cortex the most affected area. Notably, the bulk AgNO3, caused similar adverse effects with a slightly more marked extent, also triggering apoptotic phenomena. Specifically, 7 days after exposure to both AgNPs and AgNO3, dilatation of the intercapillary and peripheral Bowman’s space was observed, together with glomerular shrinkage. At day 28, these effects still persisted after both treatments, accompanied by an additional injury involving the vascular component of the mesangium, with interstitial micro-hemorrhages. Neither AgNPs nor AgNO3 induced oxidative stress effects in kidneys and plasma, at either time point. The AgNP-induced moderate renal effects indicate that, despite their benefits, novel AgNPs employed in consumer products need exhaustive investigation to ensure public health safety.
Dose- and Ion-Dependent Effects in the Oxidative Stress Response to Space-Like Radiation Exposure in the Skeletal System
Authors: Alwood Joshua , Tran Luan , Schreurs Ann-Sofie , Shirazi-Fard Yasaman , Kumar Akhilesh , Hilton Diane , Tahimic Candice , Globus Ruth
Published: October 2017

Space radiation may pose a risk to skeletal health during subsequent aging. Irradiation acutely stimulates bone remodeling in mice, although the long-term influence of space radiation on bone-forming potential (osteoblastogenesis) and possible adaptive mechanisms are not well understood. We hypothesized that ionizing radiation impairs osteoblastogenesis in an ion-type specific manner, with low doses capable of modulating expression of redox-related genes. 16-weeks old, male, C57BL6/J mice were exposed to low linear-energy-transfer (LET) protons (150 MeV/n) or high-LET 56Fe ions (600 MeV/n) using either low (5 or 10 cGy) or high (50 or 200 cGy) doses at NASA’s Space Radiation Lab. Five weeks or one year after irradiation, tissues were harvested and analyzed by microcomputed tomography for cancellous microarchitecture and cortical geometry. Marrow-derived, adherent cells were grown under osteoblastogenic culture conditions. Cell lysates were analyzed by RT-PCR during the proliferative or mineralizing phase of growth, and differentiation was analyzed by imaging mineralized nodules. As expected, a high dose (200 cGy), but not lower doses, of either 56Fe or protons caused a loss of cancellous bone volume/total volume. Marrow cells produced mineralized nodules ex vivo regardless of radiation type or dose; 56Fe (200 cGy) inhibited osteoblastogenesis by more than 90% (5 weeks and 1 year post-IR). After 5 weeks, irradiation (protons or 56Fe) caused few changes in gene expression levels during osteoblastogenesis, although a high dose 56Fe (200 cGy) increased Catalase and Gadd45. The addition of exogenous superoxide dismutase (SOD) protected marrow-derived osteoprogenitors from the damaging effects of exposure to low-LET (137Cs γ) when irradiated in vitro, but had limited protective effects on high-LET 56Fe-exposed cells. In sum, either protons or 56Fe at a relatively high dose (200 cGy) caused persistent bone loss, whereas only high-LET 56Fe increased redox-related gene expression, albeit to a limited extent, and inhibited osteoblastogenesis. Doses below 50 cGy did not elicit widespread responses in any parameter measured. We conclude that high-LET irradiation at 200 cGy impaired osteoblastogenesis and regulated steady-state gene expression of select redox-related genes during osteoblastogenesis, which may contribute to persistent bone loss.
Offset Free Tracking Predictive Control Based on Dynamic PLS Framework
Authors: Xin Jin , Yue Wang , Lin Luo
Published: October 2017

This paper develops an offset free tracking model predictive control based on a dynamic partial least square (PLS) framework. First, state space model is used as the inner model of PLS to describe the dynamic system, where subspace identification method is used to identify the inner model. Based on the obtained model, multiple independent model predictive control (MPC) controllers are designed. Due to the decoupling character of PLS, these controllers are running separately, which is suitable for distributed control framework. In addition, the increment of inner model output is considered in the cost function of MPC, which involves integral action in the controller. Hence, the offset free tracking performance is guaranteed. The results of an industry background simulation demonstrate the effectiveness of proposed method.
Color Texture Image Retrieval Based on Local Extrema Features and Riemannian Distance
Authors: Pham Minh-Tan , Mercier Grégoire , Bombrun Lionel
Published: October 2017

A novel efficient method for content-based image retrieval (CBIR) is developed in this paper using both texture and color features. Our motivation is to represent and characterize an input image by a set of local descriptors extracted from characteristic points (i.e., keypoints) within the image. Then, dissimilarity measure between images is calculated based on the geometric distance between the topological feature spaces (i.e., manifolds) formed by the sets of local descriptors generated from each image of the database. In this work, we propose to extract and use the local extrema pixels as our feature points. Then, the so-called local extrema-based descriptor (LED) is generated for each keypoint by integrating all color, spatial as well as gradient information captured by its nearest local extrema. Hence, each image is encoded by an LED feature point cloud and Riemannian distances between these point clouds enable us to tackle CBIR. Experiments performed on several color texture databases including Vistex, STex, color Brodazt, USPtex and Outex TC-00013 using the proposed approach provide very efficient and competitive results compared to the state-of-the-art methods.
Photocatalytic Performance of a Novel MOF/BiFeO3 Composite
Authors: Si Yunhui , Li Yayun , Zou Jizhao , Xiong Xinbo , Zeng Xierong , Zhou Ji
Published: October 2017

In this study, MOF/BiFeO3 composite (MOF, metal-organic framework) has been synthesized successfully through a one-pot hydrothermal method. The MOF/BiFeO3 composite samples, pure MOF samples and BiFeO3 samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and by UV–vis spectrophotometry. The results and analysis reveal that MOF/BiFeO3 composite has better photocatalytic behavior for methylene blue (MB) compared to pure MOF and pure BiFeO3. The enhancement of photocatalytic performance should be due to the introduction of MOF change the surface morphology of BiFeO3, which will increase the contact area with MB. This composing strategy of MOF/BiFeO3 composite may bring new insight into the designing of highly efficient photocatalysts.
Massively Parallel Sequencing of Genes Implicated in Heritable Cardiac Disorders: A Strategy for a Small Diagnostic Laboratory
Authors: Leong Ivone , Stuckey Alexander , Belluoccio Daniele , Fan Vicky , Skinner Jonathan , Prosser Debra , Love Donald.
Published: October 2017

Sudden cardiac death (SCD) in people before the age of 35 years is a devastating event for any family. The causes of SCD in the young can be broadly divided into two groups: heritable cardiac disorders that affect the heart structure (cardiomyopathies) and primary electrical disorders (cardiac ion channelopathies). Genetic testing is vital as those suffering from cardiac ion channelopathies have structurally normal hearts, and those with cardiomyopathies may only show subtle abnormalities in the heart and these signs may not be detected during an autopsy. Post-mortem genetic testing of SCD victims is important to identify the underlying genetic cause. This is important as family cascade screening may be undertaken to identify those who may be at risk and provide vital information about risk stratification and clinical management. The development of massively parallel sequencing (MPS) has made it possible for the simultaneous screening of multiple patients for hundreds of genes. In light of this, we opted to develop an MPS approach for SCD analysis that would allow us to screen for mutations in genes implicated in cardiomyopathies and cardiac ion channelopathies. The rationale behind this panel was to limit it to genes carrying the greatest mutation load. If no likely pathogenic gene variant were found then testing could cascade to whole exome/genome sequencing as a gene-discovery exercise. The overarching aim was to design and validate a custom-cardiac panel that satisfies the diagnostic requirements of LabPLUS (Auckland City Hospital, Auckland, NZ) and the guidelines provided by the Royal College of Pathologists of Australasia and the Association for Clinical Genetic Science.
Tetraphenylpyrimidine-Based AIEgens: Facile Preparation, Theoretical Investigation and Practical Application
Authors: Liu Junkai , Pan Lingxiang , Peng Qian , Qin Anjun
Published: October 2017

Aggregation-induced emission (AIE) has become a hot research area and tremendous amounts of AIE-active luminogens (AIEgens) have been generated. To further promote the development of AIE, new AIEgens are highly desirable. Herein, new AIEgens based on tetraphenylpyrimidine (TPPM) are rationally designed according to the AIE mechanism of restriction of intramolecular motion, and facilely prepared under mild reaction conditions. The photophysical property of the generated TPPM, TPPM-4M and TPPM-4P are systematically investigated and the results show that they feature the aggregation-enhanced emission (AEE) characteristics. Theoretical study shows the high-frequency bending vibrations in the central pyrimidine ring of TPPM derivatives dominate the nonradiative decay channels. Thanks to the AEE feature, their aggregates can be used to detect explosives with super-amplification quenching effects, and the sensing ability is higher than typical AIE-active tetraphenylethene. It is anticipated that TPPM derivatives could serve as a new type of widely used AIEgen based on their facile preparation and good thermo-, photo- and chemostabilities.
Phosphorus-Sulfur Heterocycles Incorporating an O-P(S)-O or O-P(S)-S-S-P(S)-O Scaffold: One-Pot Synthesis and Crystal Structure Study
Authors: Hua Guoxiong , Davidson Kate , Cordes David , Du Junyi , Slawin Alexandra , Woollins J.
Published: October 2017

A new one-pot preparative route was developed to synthesize novel organophosphorus-sulfur heterocycles via the reaction of the four-membered ring thionation reagent [2,4-diferrocenyl-1,3,2,4-diathiadiphosphetane 2,4-disulfide (FcLR, a ferrocene analogue of Lawesson’s reagent)] and alkenyl/aryl-diols and I2 (or SOCl2) in the presence of triethylamine. Therefore, a series of five- to ten-membered heterocycles bearing an O-P(S)-O or an O-P(S)-S-S-P(S)-O linkage were synthesized. The synthesis features a novel application of the multicomponent reaction, providing an efficient and environmentally benign method for the preparation of the unusual phosphorus-sulfur heterocycles. Seven representative X-ray structures confirm the formation of these heterocycles.
A Review on the Phytochemistry, Pharmacology, Pharmacokinetics and Toxicology of Geniposide, a Natural Product
Authors: Shan Mingqiu , Yu Sheng , Yan Hui , Guo Sheng , Xiao Wei , Wang Zhenzhong , Zhang Li , Ding Anwei , Wu Qinan , Li Sam
Published: October 2017

Iridoid glycosides are natural products occurring widely in many herbal plants. Geniposide (C17H24O10) is a well-known one, present in nearly 40 species belonging to various families, especially the Rubiaceae. Along with this herbal component, dozens of its natural derivatives have also been isolated and characterized by researchers. Furthermore, a large body of pharmacological evidence has proved the various biological activities of geniposide, such as anti-inflammatory, anti-oxidative, anti-diabetic, neuroprotective, hepatoprotective, cholagogic effects and so on. However, there have been some research articles on its toxicity in recent years. Therefore, this review paper aims to provide the researchers with a comprehensive profile of geniposide on its phytochemistry, pharmacology, pharmacokinetics and toxicology in order to highlight some present issues and future perspectives as well as to help us develop and utilize this iridoid glycoside more efficiently and safely.
Inhibition of 5α-Reductase, IL-6 Secretion, and Oxidation Process of Equisetum debile Roxb. ex Vaucher Extract as Functional Food and Nutraceuticals Ingredients
Authors: Chaiyana Wantida , Punyoyai Chanun , Somwongin Suvimol , Leelapornpisid Pimporn , Ingkaninan Kornkanok , Waranuch Neti , Srivilai Jukkarin , Thitipramote Natthawut , Wisuitiprot Wudtichai , Schuster Roswitha , Viernstein Helmut , Mueller Monika
Published: October 2017

This study aims to investigate the biological activities related to hair loss of Equisetum debile extracts, including 5α-reductase inhibition, interleukin-6 (IL-6) secretion reduction, and anti-oxidation. E. debile extracts were obtained by maceration in various solvents. Crude extract (CE) was obtained by maceration in 95% ethanol. Chlorophyll-free extract (CF) was the CE which of the chlorophyll has been removed by electrocoagulation. Hexane extract (HE), ethyl acetate extract (EA), and ethanolic extract (ET) were fraction extracts obtained from maceration in hexane, ethyl acetate, and 95% ethanol, respectively. The extracts were investigated for inhibitory activity against 5α-reductase and IL-6 secretion. Total phenolic contents (TPC) were investigated and antioxidant activities were determined by means of 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The inhibition of lipid peroxidation was determined by the ferric thiocyanate method. The cytotoxicity of the extracts on dermal papilla cells and irritation test by hen's egg test chorioallantoic membrane assay were also investigated. All extracts could inhibit 5α-reductase and decrease IL-6 secretion in lipopolysaccharide-stimulated macrophage. The antioxidant activity of E. debile extracts was directly related to their TPC. ET which contained the highest TPC (68.8 ± 6.7 mg GA/g) showed the highest equivalent concentration (EC1) of 289.1 ± 26.4 mM FeSO4/g, TEAC of 156.6 ± 34.6 mM Trolox/g, and 20.0 ± 6.0% DPPH inhibition. However, EA exhibited the highest inhibition against lipid peroxidation (57.2 ± 0.4%). In addition, EA showed no cytotoxicity on dermal papilla cell line and no irritation on chorioallantoic membrane of hen’s eggs. In conclusion, EA was suggested as the most attractive ingredients for functional food and nutraceuticals because of the high inhibitory activity against 5α-reductase, IL-6 secretion, and lipid peroxidation inhibition.