bluuebunny/crossref_metadata_2025 huggingface dataset:
Occupational Therapy Research Publications From 2001 to 2020 in PubMed: Trends and Comparative Analysis with Physiotherapy and Rehabilitation
Authors: Shepherd Heather A. , Jesus Tiago S. , Nalder Emily , Dabbagh Armaghan , Colquhoun Heather
Published: November 2024

A limited understanding of trends in occupational therapy (OT) research publications exists. This study aimed to evaluate trends in OT research publications, in PubMed (2001–2020), compared to physiotherapy and rehabilitation. A method of secondary analysis of publication trends in the PubMed database was used. Medical subject headings for OT, physiotherapy, and rehabilitation were combined with search filters (e.g., population age, study design, and OT practice area). Linear regressions were computed to analyze changes in yearly growth. OT research publications increased by 5.86 per year and comprised less than 2.5% of rehabilitation research publications yearly. Knowledge synthesis was the predominant OT study design (2.94% yearly increase; p < .001). Intellectual/cognitive conditions and emergent practice areas in OT research publications increased over time (both p = .007). OT research publications were relatively evenly distributed across population age. OT research publications are increasing over time but lag relative to physiotherapy and rehabilitation broadly. Our findings may inform future OT research priorities.
Human-Machine symbiosis in educational leadership in the era of artificial intelligence (AI): Where are we heading?
Authors: Arar Khalid , Tlili Ahmed , Salha Soheil
Published: November 2024

The issue of Artificial Intelligence (AI) and educational leadership has become a central topic following the shift to knowledge technology and digital literacy in recent years. Despite increasing attention to the topic among scholars and policymakers during the last decade, we lack a comprehensive review of AI and educational leadership. Therefore, by using bibliometric analysis of data derived from the Scopus database, this paper represents a systematic scoping of AI and educational leadership, tracing its development, characteristics, and knowledge accumulation within broad perspectives. Results identify key trends, geographic representation, topical foci, main concepts, themes, and their interconnections, including their implications for targeted Sustainable Development Goals. Implications, limitations, and future research reviewed in the studies are fully discussed.
Knowledge, attitudes, and self-reported practices regarding plagiarism among health researchers in Egypt and Lebanon: a multicenter questionnaire study
Authors: Abdelnaby Asmaa , Abbas Nivine , Abdelhafiz Ahmed Samir , Mohammed Zeinab , Yasser Sara , Taha Nada , Makkeyah Sara , Mohammed Maha , Silverman Henry J.
Published: November 2024

To enhance efforts against plagiarism, a deeper understanding of its various aspects is essential. This cross-sectional questionnaire study assessed the knowledge, attitudes, subjective norms, and self-reported plagiarism practices among health researchers in Egypt and Lebanon. A validated questionnaire was distributed to postgraduate students and faculty members across 15 universities through personal emails and health researcher-specific social media platforms. Out of 283 respondents, the mean knowledge score was 14.8 ± 3.7 out of a possible 27.0, indicating moderate knowledge of plagiarism. Attitudes toward plagiarism were moderately permissive, with an average score of 62.2 ± 10.9, out of a maximum of 90.0. Subjective norms received a moderate score of 33.6 ± 6.7, where a favorable score should be low (10–23) suggesting that participants perceive society as disapproving of plagiarism and believe its prevalence is low. The mean self-reported Plagiarism Severity Score (PSS) was 4.1 ± 3.6, reflecting a low level of self-reported plagiarism, with a maximum possible score of 18. Multivariable analysis revealed a negative correlation between subjective norms and the PSS (β = −0.194, CI [−0.196: −0.010]). This finding is counterintuitive, as one would typically expect that a lower subjective norms score (indicating stronger disapproval of plagiarism), would correlate with fewer acts of plagiarism. One possible explanation for this result is the presence of negative perceptions of organizational justice—such as feelings of being unfairly treated—which might lead to higher rates of self-reported research misconduct. We recommend that universities in the Arab Middle East region develop research ethics training programs that include responsible conduct in research. However, it is essential for academic leaders to assess and address the research integrity climate within their institutions, as organizational injustice could undermine the effectiveness of these educational efforts.
Altercentrism in perspective-taking: The role of humanisation in embodying the agent’s point of view
Authors: Conson Massimiliano , Zappullo Isa , Cordasco Gennaro , Trojano Luigi , Raimo Gennaro , Cecere Roberta , Baiano Chiara , Lauro Anna , Esposito Anna
Published: November 2024

We investigated the role of humanisation in Visual Perspective-Taking (VPT) by testing whether and how agent’s human-likeness and attractiveness (“hedonic quality”) interact with social cues (action and eye gaze) in influencing the participants’ disposition to embody another’s perspective. In a VPT task, participants viewed scenes displaying an actor (human or robotic) grasping, gazing (or both) a target object, or adopting a still posture, and were required to judge the left/right location of the target, without receiving any instruction on the perspective to be assumed. Across two studies, we selected human and robotic agents to use as actors in the VPT task. Results consistently demonstrated that participants could be effectively clustered by a data-driven method into two perspective-taking styles, depending on the presence of a systematic tendency to locate the target object in the VPT scenarios from own (egocentric) or the actor’s (altercentric) point of view. The human versus nonhuman nature of the agent seemed able to affect the participants’ egocentric or altercentric tendency whereas both the agent’s hedonic quality and social cues were not able to influence this propensity. Identifying the factors influencing altercentrism during human–robot interactions can be essential for developing artificial agents favouring user’s acceptance and willingness to interact. In this respect, considering differences among individuals in their propensity to take another’s point of view may be of central importance. Clustering approaches can represent a useful means to capture interindividual differences in this central aspect of human social cognition.
When the going gets tough: Educational leadership and resilience in times of crises
Authors: Hill-Berry Nola P , Burris-Melville Tashieka S
Published: November 2024

Managing and leading under challenging conditions is one of the hallmarks of effective leadership. In today’s educational environment, educational leaders and managers must be swift-thinking and fast-acting in their approach. This demands building resilience. In this mixed-methods study, the researchers explored educational leadership and resilience during crises. The study sought to answer two overarching questions: What lessons did educational leaders learn from the COVID-19 experience, and How do the lessons learned prepare educational leaders to be resilient in times of crises? Data for this study were collected via survey and interviews. Survey data were analysed using descriptive statistics, and interview data were analysed using Taylor-Powell and Renner’s (2003) five-step approach. The major findings revealed a necessity for proactive planning, effective communication, and a team approach. Leaders highlighted the importance of resilience training, preparation, and emotional intelligence in navigating crises. The findings provide valuable information that could be used to equip educational leaders and managers to build resilience and augment the practice of educational leadership, particularly during times of crises. Therefore, the recommendations emphasise the need for comprehensive crisis leadership training, the development of contingency plans, and continuous professional development to enhance leaders’ capacity to manage future disruptions effectively.
Performance of ChatGPT on prehospital acute ischemic stroke and large vessel occlusion (LVO) stroke screening
Authors: Wang Xinhao , Ye Shisheng , Feng Jinwen , Feng Kaiyan , Yang Heng , Li Hao
Published: November 2024

Background

The management of acute ischemic stroke (AIS) is time-sensitive, yet prehospital delays remain prevalent. The application of large language models (LLMs) for medical text analysis may play a potential role in clinical decision support. We assess the performance of LLMs on prehospital AIS and large vessel occlusion (LVO) stroke screening.

Methods

This retrospective study sourced cases from the electronic medical record database of the emergency department (ED) at Maoming People's Hospital, encompassing patients who presented to the ED between June and November 2023. We evaluate the diagnostic accuracy of GPT-3.5 and GPT-4 for the detection of AIS and LVO stroke by comparing the sensitivity, specificity, accuracy, positive predictive value, negative predictive value, and positive likelihood ratio and AUC of both LLMs. The neurological reasoning of LLMs was rated on a five-point Likert scale for factual correctness and the occurrence of errors.

Result

On 400 records from 400 patients (mean age, 70.0 years ± 12.5 [SD]; 273 male), GPT-4 outperformed GPT-3.5 in AIS screening (AUC 0.75 (0.65–0.84) vs 0.59 (0.50–0.69), P = 0.015) and LVO identification (AUC 0.71 (0.65–0.77) vs 0.60 (0.53–0.66), P < 0.001). GPT-4 achieved higher accuracy than GPT-3.5 in screening of AIS (89.3% [95% CI: 85.8, 91.9] vs 86.5% [95% CI: 82.8, 89.5]) and LVO stroke identification (67.0% [95% CI: 62.3%, 71.4%] vs 47.3% [95% CI: 42.4%, 52.2%]). In neurological reasoning, GPT-4 had higher Likert scale scores for factual correctness (4.24 vs 3.62), with a lower rate of error (6.8% vs 24.8%) than GPT-3.5 (all P < 0.001).

Conclusions

The result demonstrates that LLMs possess diagnostic capability in the prehospital identification of ischemic stroke, with the ability to exhibit neurologically informed reasoning processes. Notably, GPT-4 outperforms GPT-3.5 in the recognition of AIS and LVO stroke, achieving results comparable to prehospital scales. LLMs are supposed to become a promising supportive decision-making tool for EMS practitioners in screening prehospital stroke.
American Foregut Classification is Superior to Hill Classification in Evaluation of the Gastroesophageal Junction
Authors: Swei Eric , Almario Jose A. , Abou Diwan Elizabeth , Ashhab Ashraf A. , Canto Marcia I.
Published: November 2024

Background:

The American Foregut Society (AFS) classification is intended to be more comprehensive and accurate than the Hill grade in assessing the gastroesophageal junction. However, real-world data regarding predictive ability and interobserver variability are limited.

Methods:

We performed a multi-reader validation study on images collected from patients who underwent endoscopic evaluation of reflux with hernia provocation using both AFS and Hill classification. The primary outcome was inter-reader agreement, assessed using Fleiss K. Secondary outcomes were percentage of patients with pathologic reflux at each Hill grade or AFS grade, correlation between acid exposure time (AET) and individual components of the AFS grade, and difference in predictive ability based on expert versus non-expert graders.

Results:

Sixty-four eligible patients were identified. Inter-rater reliability was substantial for AFS classification ( K = 0.65) and fair for Hill classification ( K= 0.28). The AFS grade demonstrated moderate overall correlation with AET (ρ = .36) and the Hill grade demonstrated overall weak correlation (ρ = .28). No single component of the AFS score performed significantly better in predicting pathologic reflux than the AFS grade (AFS F = 9.93, P = .0025) and there was no significant difference in overall grading between expert- and non-expert readers (ρ = .38 vs ρ = .29).

Conclusion:

The AFS score can classify the GEJ with moderate interobserver agreement and is superior to the Hill grade in predicting acid exposure time. AFS grade has better predictive power than the individual components used in scoring and is robust to differences in reader experience.
Dexmedetomidine alleviates intestinal ischemia/reperfusion injury by modulating intestinal neuron autophagy and mitochondrial homeostasis via Nupr1 regulation
Authors: Wu Qiong , Chen Qiuhong , Liang Sisi , Nie Jinping , Wang Yingjie , Fan Chenlu , Liu Zhen , Zhang Xuekang
Published: November 2024

Intestinal ischemia/reperfusion injury (I/R) is a common yet challenging-to-treat condition, presenting a significant clinical challenge. This study aims to investigate the protective mechanisms of Dexmedetomidine (Dex) against I/R injury, with a particular focus on its role in regulating autophagy activity in intestinal neurons and maintaining mitochondrial homeostasis. Experimental findings demonstrate that Dex can mitigate intestinal damage induced by I/R through the modulation of autophagy activity and mitochondrial function in intestinal neurons by suppressing the expression of Nupr1. This discovery sheds light on a new molecular mechanism underlying the potential efficacy of Dex in treating intestinal I/R injury, offering valuable insights for clinical therapy.
Genetic diversity assessment of cucumber landraces using molecular signatures
Authors: Iftikhar Muhammad Sarmad , Naseer Cheema Hafiza Masooma , Khan Asif Ali , DeLacy Ian Henson , Basford Kaye Enid
Published: November 2024

Genetic profiling of the biodiversity in cultivated crop plants is necessary to preserve important genes and utilize them in a breeding program. Cucumber is used as a model plant to study various characteristics of Cucurbitaceae. Its adaptation to a wide range of climatic conditions suggested analyzing the landraces. The present study was conducted to evaluate the differences, at the genetic level, among landraces spanning five continents. DNA extracted from fifty-six landraces selected from USDA germplasm bank to cover a global representative sample of world cucumber landraces was used for polymerase chain reaction using twenty-eight polymorphic expressed sequence tags simple sequence repeat (EST-SSR) markers. Twenty-eight EST-SSR markers covering all seven chromosomes yielded 98 bands with an average of 3.42 bands per marker. Polymorphic information content ranged from 0.00 (EC35) to 0.74 (EC17) with an average of 0.34. Six clusters provided an appropriate summary of the variation among the landraces, with the two largest groups including 32 (Asiatic) and 17 (European and American) landraces, respectively. Four small groups, three with two members, and one with one member (PI 525155-Egypt) were dissimilar to the two main groups. Landraces from the same region were often clustered together. Genetic similarity of the landraces was revealed by marker banding patterns. The locations of genetic diversity for cucumber landraces can be identified from this study.
Toward a comparative understanding of beat perception and synchronization
Authors: Schmidt Marc F , Kaplan Michael
Published: November 2024

BPS provides unique opportunities for comparative studies to understand the mechanism(s) of beat perception and its translation into synchronous motor output. We argue that an evolutionary perspective of this behavior will require a clearer dissociation between beat perception and synchronization and a more parameterized approach to studying this behavior.
The “coordination conjecture” as an alternative to Patel’s fortuitous enhancement hypothesis for the relation between vocal learning and beat-based dancing
Authors: Hickok Gregory
Published: November 2024

Patel proposes a viable hypothesis regarding the relation between vocal learning and beat-based dancing but it is not without problems. I highlight these problems and propose a solution, the “coordination conjecture.”
De-sync: disruption of synchronization as a key factor in individual and collective creative processes
Authors: Laroche Julien , Bachrach Asaf , Noy Lior
Published: November 2024

Creativity is a key skill for the twenty-first century, where the individual and collective imperative to adapt is omnipresent. Yet, it is still unclear how to put creativity theories into practice, which signals a lacuna in our understanding of the pragmatic means by which we get creative. This paper starts from the identification of a number of gaps in the literature. In particular, individual and group creativity are usually treated separately, and the emphasis on the search for novelty seems to overshadow the importance experts give to the disruption of their habitual patterns of behavior. To overcome these gaps, we propose foundations for a unifying framework that takes the perspective of dynamical systems. Specifically, we suggest that de-synchronization, a hallmark of disruption, is an integral part of the creative processes that operate across individual and collective levels of analysis. We show that by conjuring uncertainty, de-synchronized states provide opportunities for creative reorganization. In order to ground this framework, we survey and discuss existing literature, and focus on group improvisation practices (in particular, music and dance improvisation), where partners use the dynamics of their interaction to bring forth a collective performance in real-time. In these practices, disruption by de-synchronization, termed here as ‘problematization of coordination’, is a pragmatic approach used to push the creative process forward. We suggest that this approach might also be relevant in other types of individual and collective creative processes.
Dance as mindful movement: a perspective from motor learning and predictive coding
Authors: Fitch W. Tecumseh , Barnstaple Rebecca
Published: November 2024

Defining “dance” is challenging, because many distinct classes of human movement may be considered dance in a broad sense. Although the most obvious category is rhythmic dancing to a musical beat, other categories of expressive movement such as dance improvisation, pantomime, tai chi, or Japanese butoh suggest that a more inclusive conception of human dance is needed. Here we propose that a specific type of conscious awareness plays an overarching role in most forms of expressive movement and can be used to define dance (in the broad sense). We can briefly summarize this broader notion of dance as “mindful movement.” However, to make this conception explicit and testable, we need an empirically verifiable characterization of “mindful movement.” We propose such a characterization in terms of predictive coding and procedural learning theory: mindful movement involves a “suspension” of automatization. When first learning a new motor skill, we are highly conscious of our movements, and this is reflected in neural activation patterns. As skill increases, automatization and overlearning occurs, involving a progressive suppression of conscious awareness. Overlearned, habitual movement patterns become mostly unconscious, entering consciousness only when mistakes or surprising outcomes occur. In mindful movement, this automatization process is essentially inverted or suspended, reactivating previously unconscious details of movement in the conscious workspace, and crucially enabling a renewed aesthetic attention to such details. This wider perspective on dance has important implications for potential animal analogs of human dance and leads to multiple lines of experimental exploration.
APOE4 rat model of Alzheimer’s disease: sex differences, genetic risk and diet
Authors: Colarusso Bradley , Ortiz Richard , Yeboah Julian , Chang Arnold , Gupta Megha , Kulkarni Praveen , Ferris Craig F.
Published: November 2024

The strongest genetic risk factor for Alzheimer’s disease (AD) is the ε4 allele of apolipoprotein E (ApoE ε4). A high fat diet also adds to the risk of dementia and AD. In addition, there are sex differences as women carriers have a higher risk of an earlier onset and rapid decline in memory than men. The present study looked at the effect of the genetic risk of ApoE ε4 together with a high fat/high sucrose diet (HFD/HSD) on brain function in male and female rats using magnetic resonance imaging. We hypothesized female carriers would present with deficits in cognitive behavior together with changes in functional connectivity as compared to male carriers. Four-month-old wildtype and human ApoE ε4 knock-in (TGRA8960), male and female Sprague Dawley rats were put on a HFD/HSD for four months. Afterwards they were imaged for changes in function using resting state BOLD functional connectivity. Images were registered to, and analyzed, using a 3D MRI rat atlas providing site-specific data on 173 different brain areas. Resting state functional connectivity showed male wildtype had greater connectivity between areas involved in feeding and metabolism while there were no differences between female and male carriers and wildtype females. The data were unexpected. The genetic risk was overshadowed by the diet. Male wildtype rats were most sensitive to the HFD/HSD presenting with a deficit in cognitive performance with enhanced functional connectivity in neural circuitry associated with food consumption and metabolism.
Providers’ perspective on vaginal birth after cesarean birth: a qualitative systematic review
Authors: Kanjanakaew Antita , Jiramanee Atchareya , Srimoragot Manassawee
Published: November 2024

Background

Vaginal Birth after Cesarean Birth (VBAC) is a birth mode recommended for reducing repeat cesarean which potentially contributes to adverse outcomes. However, VBAC is not normally practiced in some countries. Providers are an important part of the decision-making process on modes of birth among pregnant individuals. Providers’ perspective on VBAC can influence whether they support or avoid conducting VBAC. This review aimed to explore providers’ perspective on VBAC.

Methods

The comprehensive search was conducted from six databases including PubMed, MEDLINE, Scopus, Cochrane Library, EMBASE, and Google scholar. The studies published in English between 2013 and 2023 were review. The Medical Subject Heading terms for VBAC and perspective were used to search. The eligible studies were selected by the PRISMA flow chart. The initial search yielded 558 articles. After excluding duplicates, articles not retrieved for full-text, and not meeting inclusion and exclusion criteria, eight articles were recruited. Quality appraisal of the studies was performed by the tool of the Joanna Briggs Institute. The meta-aggregation approach was applied to synthesize the findings.

Results

Eight qualitative articles were included in this review, and six themes were developed including (1) different recognition of VBAC, (2) differences of willingness level of conducting Trial of Labor after Cesarean (TOLAC) (the approach attempting to have VBAC), (3) skills and resources needed when performing TOLAC, (4) protocol for recruiting candidacy and TOLAC management, (5) final decision making on VBAC, and (6) onset and duration of providing TOLAC information.

Conclusion

Providers play an important role in influencing individuals’ decision on modes of birth. Providers’ positive recognition and willingness of conducting TOLAC potentially impact successful VBAC rate. However, the lawsuit caused by adverse outcomes from TOLAC/VBAC is a main reason for choosing repeat cesarean.

Trial registration

PROSPERO registration number of this systematic review: CRD42023427662.
Prevalence and factors associated with cerebral malaria among children aged 6 to 59 months with severe malaria in Western Uganda: a hospital-based cross-sectional study
Authors: Mseza Banga , Kumbowi Patrick Kumbakulu , Nduwimana Martin , Banga Desire , Busha Emmanuel Tibasima , Egesa Walufu Ivan , Odong Richard Justin , Ndeezi Grace
Published: November 2024

Introduction

Cerebral malaria, caused by Plasmodium falciparum, represents the most severe neurologic complication of malaria. Its association with high morbidity and mortality rates, especially among young children, underscores its clinical significance. In sub-Saharan Africa, including Uganda, cerebral malaria remains a major health challenge, contributing significantly to the high child mortality rate. Despite advances in malaria control, the burden of cerebral malaria among children under five is substantial, reflecting the need for targeted interventions and improved management strategies. This study aimed to determine the prevalence of cerebral malaria and identify associated factors among children admitted with severe malaria at a tertiary hospital in western Uganda.

Methods

This was a cross-sectional, descriptive, and analytical study involving children aged 6 to 59 months admitted with severe malaria. The study was conducted from January to March 2023 at Fort Portal Regional Referral Hospital. Severe and cerebral malaria were defined as per the WHO criteria. Sociodemographic, clinical, and laboratory data were collected and analyzed using IBM SPSS version 27. Logistic regression analysis was used to evaluate the factors associated with cerebral malaria. A p-value < 0.05 indicated statistical significance.

Results

A total of 250 children were recruited (mean age 33.1 ± 17.3 months). The prevalence of cerebral malaria was 12.8% (95% CI: 8.9–17.6). Cerebral malaria was independently associated with male sex (aOR: 3.05, 95% CI: 1.20–7.77, p = 0.02), abnormal bleeding (aOR: 13.22, 95% CI: 11.54–15.16, p = 0.001), history of convulsions (aOR 12.20, 95% CI: 10.7–21.69, p = 0.010), acute kidney injury (aOR: 4.50, 95% CI: 1.30–15.53, p = 0.02), and hyponatremia (aOR: 3.47, 95% CI: 1.34–8.96, p = 0.010).

Conclusions and recommendations

The prevalence of cerebral malaria was high among children with severe malaria. Factors associated with cerebral malaria included male gender, history of convulsions, abnormal bleeding, acute kidney injury, and hyponatremia. Targeted interventions and early management are essential to improve clinical outcomes.
CILP-2 expression in the intervertebral discs of patients with lumbar radiculopathy
Authors: Kõiv K. , Aunapuu M. , Torga T. , Rätsep T. , Bakhoff K. , Arend A.
Published: November 2024

Background

Intervertebral disc (IVD) degeneration (IVDD) is one of the main causes of low back pain. One of the most important features of IVDD is the loss of extracellular matrix (ECM) with its structural components. Cartilage intermediate layer proteins (CILPs), minor glycoproteins residing in ECM, have been found to be increased in IVD as degeneration and aging progresses. The aim of the present study was to evaluate the expression of CILP-2 in the IVD of patients with lumbar radiculopathy.

Methods

The IVD samples were collected from 25 patients during spinal surgery (interlaminectomy, herniated disc removal). The control IVD samples were obtained from nine patients who underwent lateral corpectomies in the thoracic region. CILP-2 expression was detected by immunohistochemistry. The patients were divided into two groups – aged under or over 50 years. A standardized clinical examination with assessment of radicular signs and deficits was performed. Subjective disability and pain were assessed using the visual analogue scale and Oswestry Disability Index (ODI). The pre-operative MRI was graded for the degree of IVD degeneration by Pfirrmann grading system. IVD samples obtained during operations were subjected to the standardized histopathological analysis applying modified Boos classification. The data were analysed by t-test, Mann-Whitney U-test, and Spearman correlation test.

Results

Both histopathology scores and Pfirrmann grades did not differ between patients’ groups. Also, no correlations were found between histopathology and Pfirrmann grades, neither were any differences seen when correlating both grades to ODI, back pain or leg pain scores. CILP-2 staining was noted in all studied samples, notably strong staining was seen around large cell clusters. However, no differences in CILP-2 staining were seen between the age groups of patients. No correlations were found between CILP-2 staining and Pfirrmann grades. Grading of CILP-2 immunostaining in nine control patient samples resulted in significantly lower values. The difference is statistically significant (P = 0.002) compared to CILP-2 staining scores of all 25 patients’ samples.

Conclusion

In this study, we detected increased CILP-2 expression in the human IVD as compared to the control group patients. CILP-2 can be a possible IVDD marker; however, as knowledge about the role of CILP-2 is limited, further studies are required.
Identifying barriers and opportunities to facilitate the uptake of whole genome sequencing in paediatric haematology and oncology practice
Authors: Bishop Michelle , Vedi Aditi , Bowdin Sarah , Armstrong Ruth , Bartram Jack , Bentley David , Ross Mark , Hook C. Elizabeth , Yin Chung Brian Hon , Moss Parker , Rowitch David H. , Tarpey Patrick , Behjati Sam , Murray Matthew J.
Published: November 2024

Background

The clinical utility of whole genome sequencing (WGS) in paediatric cancer has been demonstrated in recent years. WGS has been routinely available in the National Health Service (NHS) England for all children with cancer in England since 2021, but its uptake has been variable geographically. To explore the underlying barriers to routine use of WGS in this population across England and more widely in the United Kingdom (UK) and the Republic of Ireland (ROI), a one-day workshop was held in Cambridge, United Kingdom in October 2022.

Methods

Following a series of talks, delegates participated in open, round-table discussions to outline local and broader challenges limiting routine WGS for diagnostic work-up for children with cancer in their Principal Treatment Centres (PTCs) and Genomic Laboratory Hubs (GLHs). Within smaller groups, delegates answered structured questions regarding clinical capability, education and training needs, and workforce competence and requirements. Data was recorded, centrally collated, and analysed following the event using thematic analysis.

Results

Sixty participants attended the workshop with broad representation from the 20 PTCs across the UK and ROI and the seven GLHs in England. All healthcare professionals involved in the WGS pathway were represented, including paediatric oncologists, clinical geneticists, clinical scientists, and histopathologists. The main themes highlighted by the group in ensuring equitable access to WGS identified were: lack of knowledge equity between NHS trusts, with a perception of WGS being for research only; and perception of lack of financial support for the clinical process surrounding WGS, including lack of time to take informed consent from patients. The latter also included limited trained staff available for data interpretation, affecting the turnaround time for reporting. Finally, the need for an integrated digital pathway to order, track, and return data to clinicians was highlighted.

Conclusion

At the workshop, the general motivation for including WGS in the diagnostic work up for children with cancer was high throughout the UK, however a perceived lack of resources and education opportunities limit the widespread use of this commissioned assay. This workshop has led to some recommendations to increase access to WGS in this population in England and more widely in the devolved national of the UK and the ROI.
Iron deficiency in dogs suffering from atopic dermatitis
Authors: Ramos Carolina Frizzo , Doulidis Pavlos G. , Polakova Nina , Burgener Iwan A. , Jensen-Jarolim Erika , Cimarelli Giulia , Panakova Lucia , Roth-Walter Franziska
Published: November 2024

Background

Iron-deficiency is associated with increased morbidity and mortality in non-communicable diseases. However, iron parameters are rarely assessed in dogs. Here, we aimed to assess and correlate iron parameters in dogs suffering from Canine Atopic Dermatitis (CAD) compared to non-atopic, healthy dogs.

Results

For this retrospective study, blood values and sera of 34 dogs with confirmed CAD were compared with 94 healthy non-atopic dogs. In our cohort, dogs with CAD had significantly lower mean corpuscular volume (MCV, ) mean corpuscular hemoglobin (MCH) but higher white blood cell counts due to increased levels of circulating neutrophils and monocytes. CAD patients also had elevated total protein and c-reactive protein (CRP), but lower albumin levels compared to our healthy control dogs, indicated low-grade inflammation in the CAD cohort. Spearman correlations associated negatively clinical symptom (CADESI-4/PVAS) with MCV; ceruloplasmin and hepcidin, but positively with serum iron. Only in the CAD-cohort, MCV, CRP and albumin-levels negatively affected serum iron-levels and were positively associated with ceruloplasmin. Linear regression analysis revealed that serum iron-levels in CAD subjects, were positively dependent on hematocrit (packed cell volume, PCV) and albumin, and negatively dependent with white blood cells and neutrophils numbers. In contrast, in the healthy cohort, hepcidin was the sole factor associated with serum iron.

Conclusions

A decreased iron status was associated with a higher symptom burden. Iron homeostasis differed markedly in healthy and atopic dermatitis dogs. CAD patients had depleted iron-stores and presented themselves with subclinical inflammation.
Reducing the harms of cannabis use in youth post-legalization: insights from Ontario youth, parents, and service providers
Authors: Kourgiantakis Toula , Hamilton Angie , Tait Christine , Tekirdag Kosar A. Kumsal , Lau Carrie K. Y. , McNeil Sandra , Lee Eunjung , Craig Shelley , Goldstein Abby L.
Published: November 2024

Background

Canada has one of the highest prevalence of cannabis use globally, particularly among young adults aged 20–24 (50%) and youth aged 16–19 (37%). In 2018, Canada legalized recreational cannabis with the aim of protecting youth by restricting their access and raising public awareness of health risks. However, there has been limited qualitative research on the perceptions of harms associated with youth cannabis use since legalization, which is crucial for developing effective harm reduction strategies. This qualitative study examined perceptions of cannabis use among youth from the perspectives of youth, parents, and service providers. We explored how participants described the perceived risks or harms associated with youth cannabis use, as well as how they described their own and others’ approaches to reducing cannabis-related risks and harms.

Methods

This qualitative study used a community-based participatory research approach in partnership with Families for Addiction Recovery (FAR), a national charity founded by parents of youth and young adults with addiction issues. Virtual semi-structured interviews were conducted, and the data were analyzed using thematic analysis.

Results

The study included 88 participants from three key groups (n = 31 youth, n = 26 parents, n = 31 service providers). Two main themes emerged regarding perceived risks or harms associated with cannabis use: (1) concerns about cannabis-related risks and harms, including addiction, brain development, impact on family, and various adverse effects on areas such as motivation, concentration, finances, employment, education, physical and mental health; and (2) minimization of risks and harms, featuring conflicting messages, normalization, and perceptions of cannabis being less harmful than other substances. Additionally, two themes related to harm reduction approaches were identified: (1) implementation of harm reduction, and (2) challenges in implementing a harm reduction approach. Specific challenges for each participant group were noted, along with structural barriers such as unavailable and inaccessible services, easy access to cannabis, inadequate public education, and insufficient information on lower-risk cannabis use guidelines.

Conclusions

Youth cannabis use is a significant public health concern that requires a multi-pronged approach. Developing youth-centered harm reduction strategies that recognize the developmental needs and vulnerabilities of youth, as well as the important role of families, is imperative.
Hand-washing at critical times and associated factors among mothers of children under-five in Ethiopia: a systematic review and meta-analysis
Authors: Sisay Gizaw , Eshete Negasa , Genetu Kassa
Published: November 2024

Good hand-washing practice is the most important preventive measure to reduce the spread of communicable disease to under-five children. Prior studies on good handwashing practice at critical times among mothers of children under-five age in Ethiopia were extremely varied. Therefore, this meta-analysis aimed to assess the overall proportion of good handwashing practice at critical times and identify the associated factors in Ethiopia. A comprehensive search of relevant studies was performed using distinct databases. Data were extracted using Microsoft Excel spreadsheet, and exported to Stata-MPv-17 for analysis. A random-effect model was used to estimate the overall proportion of good hand-washing practice at critical times. A random or fixed effects model was used to compute the pooled AORs with their 95% Cis, which were presented on a forest plot. $${I}^{2}$$

I

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test statistics was used for assessing heterogeneity among the included studies. The funnel plot and Egger's test were used to assess the publication bias. Ten studies were included in this systematic review and meta-analysis. The overall pooled proportion of good hand-washing practice at critical times among mothers of children under-five age in Ethiopia was 43.18% (95% CI = 31.05, 55.31). Insufficient water (AOR = 0.33, 95% CI: 0.13, 0.52), good knowledge (AOR = 1.35, 95% CI = 1.09, 1.61), desirable attitude (AOR = 4.34, 95% CI = 1.84, 6.84) and higher educational level (AOR = 0.17, 95% CI = 0.08, 0.25) were significantly associated with good practice of hand-washing at critical times. The national level of good practice of hand-washing at critical times was low. Hence, to promote good practice of hand-washing, it is essential to increase the accessibility of water and provide hygiene health education and training programs to improve knowledge and desirable attitudes of mothers.
Population dynamics of sympatric Phortica spp. and first record of stable presence of Phortica oldenbergi in a Thelazia callipaeda-endemic area of Italy
Authors: Bernardini Ilaria , Poggi Cristiana , Porretta Daniele , Máca Jan , Perugini Eleonora , Manzi Sara , Gabrielli Simona , Pichler Verena , Latrofa Maria Stefania , Fourie Josephus , Lia Riccardo Paolo , Beugnet Frédéric , Otranto Domenico , Pombi Marco
Published: November 2024

Background

Five species of the Phortica genus (Diptera: Drosophilidae) are known in Europe and the Middle East. Among these, Phortica variegata and Phortica okadai are better known for their role as vectors of the zoonotic eyeworm Thelazia callipaeda. Other species, such as Phortica semivirgo and Phortica oldenbergi, have been studied less. Given the paucity of data about these Phortica spp. vectors, we explored the population dynamics and ecology of Phortica spp. in an area highly endemic for T. callipeada (Manziana, Rome, Central Italy).

Methods

Phortica spp. flies were collected over a 3-year period (2018–2020) during their active season (April–October) with a sweep net while hovering around fermenting fruits or a human operator acting as baits. Collected flies were morphologically identified and tested for a T. callipeada infection and for the presence of Wolbachia, by polymerase chain reaction (PCR). Population dynamics of species collected was associated to environmental drivers through generalized additive models.

Results

Of the 5564 flies collected, 90.8% were P. variegata, 9.1% were P. oldenbergi, 0.05% were P. semivirgo, and one specimen was P. okadai. Only P. variegata scored molecularly infected with T. callipeada throughout the 3-year sampling period (1.8%). Phortica oldenbergi, observed consistently during the entire sampling period, exhibited a marked preference for fruit traps, contrasting with the lachryphagous activity of P. variegata. Analysis of environmental drivers of P. oldenbergi and P. variegata population dynamics indicated temperature, wind speed, and pressure as significant factors. In addition, Wolbachia pipientis endosymbiont was detected in P. oldenbergi and P. okadai.

Conclusions

For the first time, this study analysed several ecological aspects of Phortica species coexisting in a T. callipeada endemic area, highlighting different behaviors in the same environment and their vectorial role. Notably, this is also the first report of the presence of P. oldenbergi in Italy and P. okadai in Europe, underscoring the importance of extensive sampling for detecting potential vectors and alien species with direct implications for vector-borne disease epidemiology.

Graphical Abstract
Dietary supplementation with N-acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs
Authors: Cheng Linghua , Shi Zhicheng , Yue Yuan , Wang Yue , Qin Yusheng , Zhao Wei , Hu Yupei , Li Qin , Guo Min , An Lei , Wang Shumin , Tian Jianhui
Published: November 2024

Background

Exogenous gonadotropin-controlled ovarian stimulation is the critical step in animal reproductive management, such as pig, sheep, bovine and other species. It helps synchronize ovulation or stimulate multiple ovulations. However, a number of evidence indicated an unexpected decrease in pregnancy outcomes following ovarian stimulation. This study aimed to explore the underlying mechanism of the pregnancy defect and develop a practical rescue strategy.

Results

Compared with those in the control group, gilts that underwent ovarian stimulation showed a decrease in pregnancy rate, farrowing rate, and total number of piglets born. Stimulated gilts also showed an increase in estradiol (E2) levels. The supraphysiological E2 level was correlated with the decrease in the number of piglets born. Furthermore, we found that high levels of E2 impair uterine receptivity, as shown by the overproliferation of endometrial epithelial cells. In vitro mechanistic studies demonstrated that high levels of E2 hyperactivate FGF-FGFR-ERK signaling cascade in the uterine endometrium, and in turn induces overproliferation of endometrial epithelial cells. Of note, N-acetyl-L-cysteine (NAC) supplementation effectively inhibits ERK hyperphosphorylation and ameliorates endometrial epithelial overproliferation. Importantly, in vivo experiments indicated that dietary NAC supplementation, compared with ovarian stimulation group, improves the uterine receptivity in gilts, and significantly increases the pregnancy rate and total number of piglets born.

Conclusions

Ovarian stimulation-induced supraphysiological levels of E2 impairs uterine receptivity by hyperactivating FGF-FGFR-ERK signaling cascade, thereby reducing pregnancy rate and litter size. Supplementing NAC to a conventional diet for gilts ameliorates hyperactivated ERK signaling and improves uterine receptivity, thus rescuing adverse pregnancy outcomes following ovarian stimulation.

Graphical Abstract
Research needs of higher specialist trainees in psychiatry in Ireland: mixed methods study
Authors: Counihan Eimear , Carey Cornelia , Feeney Anna , Lally Kevin , O'Connor Ciara , Doherty Anne M.
Published: November 2024

Aims and method

Higher specialist trainees (HSTs) in psychiatry in Ireland were recruited to complete a 21-item online questionnaire anonymously. Questions were designed to establish the research experience of HSTs in various years of training, identify perceived barriers to participation and generate potential strategies to overcome these barriers.

Results

Of 165 HSTs surveyed, 50 (30%) responded. Most respondents (58%) were in the second or third year of HST. Most (72%) were training in general adult psychiatry. Themes that emerged from analysis of the qualitative data were ‘collaborative research culture’, ‘guidance’, ‘choice’ and ‘access to resources’. Participants felt they needed more structured guidance and regular supervision, and expressed a desire for more networking and collaboration.

Clinical implications

The need for a supportive, collaborative research culture within psychiatry was predominant among responses. Structured research programmes and access to resources may facilitate a more positive research culture and should be considered as part of the training curriculum.
Professional Tennis Players and their subsceptibility for Obsessive-Compulsive and Depressive Symptoms
Authors: Cappellato G.
Published: November 2024

Introduction

A moderate sport activity is considered beneficial for both physical and mental health. On the contrary, different studies have shown that professional players may be more vulnerable to suffer from psychological and/or psychiatric disorders.

Objectives

Given the limited information available, the present study aimed to investigate the possible presence of depressive and obsessive-compulsive symptoms or disorders in a group of professional tennis players.

Methods

Twenty-five current or former professional tennis players (18 men and 7 women; mean age ± SD: 42.32 ± 13.45 years), were recruited within the Italian Tennis Federation during an international competition and during a master meeting of coaches. They were compared with a control group, recruited from university students, doctors and nurses. All of them underwent a psychiatric interview with a structured scale and a psychopathological assessment carried out with the Mini-International Neuropsychiatric Interview (MINI), the Yale-Brown Obsessive Compulsive Scale (Y-BOCS) and the Self Assessment Scale for Depression (SAD).

Results

The Y-BOCS total and subscale scores were significantly higher in both current and past athletes than controls. Current athletes showed higher scores at Y-BOCS total, subscales and some items. The majority of the current athletes also showed superstitions and magical thinking.

Conclusions

The present study demonstrated that professional tennis players show a relevant increase of obsessive-compulsive symptoms and supertistions than controls. Interestingly, current athletes resulted more severe than past ones. Taken together, our findings support the notion that agonistic sport activities of high level require intensive training and compliance to strict daily routines that might represent a sort of vulnerability toward the onset of full-blown obsessive-compulsive disorder (as well as other disorders) in more fragile individuals. Not suprisingly, sport psychological support experts are increasingly needed.

Disclosure of Interest

None Declared