bluuebunny/crossref_metadata_2025 huggingface dataset:
NR-SAFE: a randomized, double-blind safety trial of high dose nicotinamide riboside in Parkinson’s disease
Authors: Berven Haakon , Kverneng Simon , Sheard Erika , Søgnen Mona , Af Geijerstam Solveig Amdahl , Haugarvoll Kristoffer , Skeie Geir-Olve , Dölle Christian , Tzoulis Charalampos
Published: November 2023

Nicotinamide adenine dinucleotide (NAD) replenishment therapy using nicotinamide riboside (NR) shows promise for Parkinson’s disease (PD) and other neurodegenerative disorders. However, the optimal dose of NR remains unknown, and doses exceeding 2000 mg daily have not been tested in humans. To evaluate the safety of high-dose NR therapy, we conducted a single-center, randomized, placebo-controlled, double-blind, phase I trial on 20 individuals with PD, randomized 1:1 on NR 1500 mg twice daily (n = 10) or placebo (n = 10) for four weeks. The trial was conducted at the Department of Neurology, Haukeland University Hospital, Bergen, Norway. The primary outcome was safety, defined as the frequency of moderate and severe adverse events. Secondary outcomes were tolerability defined as frequency of mild adverse events, change in the whole blood and urine NAD metabolome, and change in the clinical severity of PD, measured by MDS-UPDRS. All 20 participants completed the trial. The trial met all prespecified outcomes. NR therapy was well tolerated with no moderate or severe adverse events, and no significant difference in mild adverse events. NR therapy was associated with clinical improvement of total MDS-UPDRS scores. However, this change was also associated with a shorter interval since the last levodopa dose. NR greatly augmented the blood NAD metabolome with up to 5-fold increase in blood NAD+ levels. While NR-recipients exhibited a slight initial rise in serum homocysteine levels, the integrity of the methyl donor pool remained intact. Our results support extending the dose range of NR in phase II clinical trials to 3000 mg per day, with appropriate safety monitoring. Clinicaltrials.gov identifier: NCT05344404.
Interrogating ligand-receptor interactions using highly sensitive cellular biosensors
Authors: Funk Maximilian A. , Leitner Judith , Gerner Marlene C. , Hammerler Jasmin M. , Salzer Benjamin , Lehner Manfred , Battin Claire , Gumpelmair Simon , Stiasny Karin , Grabmeier-Pfistershammer Katharina , Steinberger Peter
Published: November 2023

Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist of fluorescent reporter cells that express chimeric receptors harboring ectodomains of cell surface molecules and intracellular signaling domains. We show that a broad range of molecules can be integrated into this platform and we demonstrate its applicability to highly relevant research areas, including the characterization of immune checkpoints and the probing of cells for the presence of receptors or ligands. The platform is suitable to evaluate the interactions of viral proteins with host receptors and to test for neutralization capability of drugs or biological samples. Our results indicate that cellular biosensors have broad utility as a tool to study protein-interactions.
Unexpected dynamics in femtomolar complexes of binding proteins with peptides
Authors: Cucuzza Stefano , Sitnik Malgorzata , Jurt Simon , Michel Erich , Dai Wenzhao , Müntener Thomas , Ernst Patrick , Häussinger Daniel , Plückthun Andreas , Zerbe Oliver
Published: November 2023

Ultra-tight binding is usually observed for proteins associating with rigidified molecules. Previously, we demonstrated that femtomolar binders derived from the Armadillo repeat proteins (ArmRPs) can be designed to interact very tightly with fully flexible peptides. Here we show for ArmRPs with four and seven sequence-identical internal repeats that the peptide-ArmRP complexes display conformational dynamics. These dynamics stem from transient breakages of individual protein-residue contacts that are unrelated to overall unbinding. The labile contacts involve electrostatic interactions. We speculate that these dynamics allow attaining very high binding affinities, since they reduce entropic losses. Importantly, only NMR techniques can pick up these local events by directly detecting conformational exchange processes without complications from changes in solvent entropy. Furthermore, we demonstrate that the interaction surface of the repeat protein regularizes upon peptide binding to become more compatible with the peptide geometry. These results provide novel design principles for ultra-tight binders.
Global genetic diversity, introgression, and evolutionary adaptation of indicine cattle revealed by whole genome sequencing
Authors: Chen Ningbo , Xia Xiaoting , Hanif Quratulain , Zhang Fengwei , Dang Ruihua , Huang Bizhi , Lyu Yang , Luo Xiaoyu , Zhang Hucai , Yan Huixuan , Wang Shikang , Wang Fuwen , Chen Jialei , Guan Xiwen , Liu Yangkai , Li Shuang , Jin Liangliang , Wang Pengfei , Sun Luyang , Zhang Jicai , Liu Jianyong , Qu Kaixing , Cao Yanhong , Sun Junli , Liao Yuying , Xiao Zhengzhong , Cai Ming , Mu Lan , Siddiki Amam Zonaed , Asif Muhammad , Mansoor Shahid , Babar Masroor Ellahi , Hussain Tanveer , Silva Gamamada Liyanage Lalanie Pradeepa , Gorkhali Neena Amatya , Terefe Endashaw , Belay Gurja , Tijjani Abdulfatai , Zegeye Tsadkan , Gebre Mebrate Genet , Ma Yun , Wang Yu , Huang Yongzhen , Lan Xianyong , Chen Hong , Migliore Nicola Rambaldi , Colombo Giulia , Semino Ornella , Achilli Alessandro , Sinding Mikkel-Holger S. , Lenstra Johannes A. , Cheng Haijian , Lu Wenfa , Hanotte Olivier , Han Jianlin , Jiang Yu , Lei Chuzhao
Published: November 2023

Indicine cattle, also referred to as zebu (Bos taurus indicus), play a central role in pastoral communities across a wide range of agro-ecosystems, from extremely hot semiarid regions to hot humid tropical regions. However, their adaptive genetic changes following their dispersal into East Asia from the Indian subcontinent have remained poorly documented. Here, we characterize their global genetic diversity using high-quality whole-genome sequencing data from 354 indicine cattle of 57 breeds/populations, including major indicine phylogeographic groups worldwide. We reveal their probable migration into East Asia was along a coastal route rather than inland routes and we detected introgression from other bovine species. Genomic regions carrying morphology-, immune-, and heat-tolerance-related genes underwent divergent selection according to Asian agro-ecologies. We identify distinct sets of loci that contain promising candidate variants for adaptation to hot semi-arid and hot humid tropical ecosystems. Our results indicate that the rapid and successful adaptation of East Asian indicine cattle to hot humid environments was promoted by localized introgression from banteng and/or gaur. Our findings provide insights into the history and environmental adaptation of indicine cattle.
3D-printed liquid metal polymer composites as NIR-responsive 4D printing soft robot
Authors: Zhang Liwen , Huang Xumin , Cole Tim , Lu Hongda , Hang Jiangyu , Li Weihua , Tang Shi-Yang , Boyer Cyrille , Davis Thomas P. , Qiao Ruirui
Published: November 2023

4D printing combines 3D printing with nanomaterials to create shape-morphing materials that exhibit stimuli-responsive functionalities. In this study, reversible addition-fragmentation chain transfer polymerization agents grafted onto liquid metal nanoparticles are successfully employed in ultraviolet light-mediated stereolithographic 3D printing and near-infrared light-responsive 4D printing. Spherical liquid metal nanoparticles are directly prepared in 3D-printed resins via a one-pot approach, providing a simple and efficient strategy for fabricating liquid metal-polymer composites. Unlike rigid nanoparticles, the soft and liquid nature of nanoparticles reduces glass transition temperature, tensile stress, and modulus of 3D-printed materials. This approach enables the photothermal-induced 4D printing of composites, as demonstrated by the programmed shape memory of 3D-printed composites rapidly recovering to their original shape in 60 s under light irradiation. This work provides a perspective on the use of liquid metal-polymer composites in 4D printing, showcasing their potential for application in the field of soft robots.
Preparation and adsorption properties of magnetic chitosan/sludge biochar composites for removal of Cu2+ ions
Authors: Zhang Meng , Liu Yunqing , Yin Zhizhen , Feng Dan , Lv Hui
Published: November 2023

The magnetic chitosan/sludge biochar composite adsorbent was prepared using chitosan, Fe3O4, and sludge biochar as raw materials. The composite adsorbent was able to achieve rapid solid–liquid separation under an applied magnetic field. The morphology and microstructure of the composite adsorbent were characterized by FTIR, XRD, SEM, VSM, and BET analysis. The adsorption performance of the composite adsorbent on Cu2+ was investigated through static adsorption experiments, and the effects of adsorbent dosage, initial concentration of Cu2+, initial pH of the solution, and adsorption temperature on the adsorption efficiency of Cu2+ were discussed. The results showed that chitosan and Fe3O4 were successfully loaded on sludge biochar. When the initial concentration of Cu2+ was 30 mg/L, the dosage of the magnetic chitosan/sludge biochar composite material was 0.05 g, the adsorption time was 180 min, pH was 5, and the temperature was room temperature, the maximum removal rate of Cu2+ reached 99.77%, and the maximum adsorption capacity was 55.16 mg/g. The adsorption kinetics and adsorption isotherm data fitted well with the pseudo-second-order kinetic model and Langmuir adsorption isotherm model, indicating that the adsorption process was chemisorption with monolayer coverage.
Quality assessment of hydroquinone, mercury, and arsenic in skin-lightening cosmetics marketed in Ilorin, Nigeria
Authors: Bamidele Olasunkanmi David , Kayode Blessing Ayomide , Eniayewu Oluwasegun Ibrahim , Adegbola Adebanjo Jonathan , Olatoye Raphael Segun , Njinga Ngaitad Stanislaus , Abdullahi Sa’ad Toyin , Bakare-Odunola Moji Taibat
Published: November 2023

Hydroquinone, Mercury (Hg), and Arsenic (As) are hazardous to health upon long-term exposure. Hydroquinone, Hg, and As were analysed in skin-lightening cosmetics randomly purchased from different cosmetic outlets within the Ilorin metropolis, Nigeria. The amount of hydroquinone in the samples was determined using a UV-spectrophotometry method at 290 nm. Hg and As were quantified using atomic absorption spectrophotometry (AAS). UV-spectrophotometry method validation showed excellent linearity (r2 = 0.9993), with limits of detection (0.75 µg/mL), limits of quantification (2.28 µg/mL), relative standard deviation (0.01–0.35%), and recovery (95.85–103.56%) in the concentration range of 5–50 µg/mL. Similarly, r2, LOD, and LOQ for Hg and As were 0.9983 and 0.9991, (0.5 and 1.0 µg/L) and 1.65 and 3.3 µg/L) respectively. All the samples contained hydroquinone, Hg and As in varying amounts. The amounts of hydroquinone, Hg and As present were in the ranges of 1.9–3.3%, 0.08–2.52 µg/g and 0.07–5.30 µg/g respectively. Only three of the analysed samples contained hydroquinone within the permissible limit of 2.0% w/w in cosmetic products. All the samples analysed contained mercury and arsenic in varying amounts. The need to periodically monitor the levels of hydroquinone, mercury, and arsenic in skin-lightening cosmetics marketed in Nigeria is recommended.
Relationship between CGM-derived nocturnal hypoglycemia and subjective sleep quality in people with type 1 diabetes
Authors: Gardner Daphne , Tan Hong Chang , Lim Gek Hsiang , Zin Oo May , Xin Xiaohui , Rama Chandran Suresh
Published: November 2023

This pilot study explores the relationship between nocturnal hypoglycemia (NH) and subjective sleep quality in people with type 1 diabetes (T1D). Twenty-seven adults with T1D wore a Freestyle Libre Pro CGM and recorded subjective sleep quality daily, as assessed by a single Likert scale question. Frequency, duration, area under the curve (AUC) of NH (00:00–06:00) defined as sensor glucose below threshold (< 3.9 mmol/L; < 3 mmol/L) for ≥ 15 min, nocturnal mean glucose, Time in Range (3.9–10 mmol/L), and coefficient of variation were calculated. Twenty-seven adults, 18 (66.7%) women, with median (IQR) age of 27 (26, 32) years and HbA1c of 7.6 (7.1, 8.1) participated. Nights with NH < 3.9 mmol/L resulted in a lower (worse) sleep score than nights without NH [Mean (SD): 3.3 (1.2) vs 3.5 (1.0), p = 0.03). A higher frequency and longer duration but not AUC [adjusted OR (95% CI) 0.52 (0.38, 0.72), 0.961 (0.932, 0.991), 0.999 (0.998, 1.001) respectively)], of NH < 3.9 mmol/L, were associated with a lower sleep score. NH < 3.0 mmol/L metrics were not associated with sleep quality. Recurrent NH < 3.9 mmol/L, rather than prolonged NH < 3.0 mmol/L, seems associated with subjective sleep quality, implying that those with the highest burden of NH are likely unaware of it.
Vanadium and tantalum doping of tin dioxide: a theoretical study
Authors: Filippatos Petros-Panagis , Kelaidis Nikolaos , Vasilopoulou Maria , Chroneos Alexander
Published: November 2023

The increasing demand of efficient optoelectronic devices such as photovoltaics has created a great research interest in methods to manipulate the electronic and optical properties of all the layers of the device. Tin dioxide (SnO2), due to his charge transport capability, high stability and easy fabrication is the main electron transport layer in modern photovoltaics which have achieved a record efficiency. While the wide band gap of SnO2 makes it an effective electron transport layer, its potential for other energy applications such as photocatalysis is limited. To further improve is conductivity and reduce its bandgap, doping or co-doping with various elements has been proposed. In the present density functional theory (DFT) study, we focus on the investigation of vanadium (V) and tantalum (Ta) doped SnO2 both in the bulk and the surface. Here we focus on interstitial and substitutional doping aiming to leverage these modifications to enhance the density of states for energy application. These changes also have the potential to influence the optical properties of the material, such as absorption, and make SnO2 more versatile for photovoltaic and photocatalytic applications. The calculations show the formation of gap states near the band edges which are beneficial for the electron transition and in the case of Ta doping the lowest bandgap value is achieved. Interestingly, in the case of Ta interstitial, deep trap states are formed which depending of the application could be advantageous. Regarding the optical properties, we found that V doping significantly increases the refractive index of SnO2 while the absorption is generally improved in all the cases. Lastly, we investigate the electronic properties of the (110) surface of SnO2, and we discuss possible other applications due to surface doping. The present work highlights the importance of V and Ta doping for energy applications and sensor applications.
Anodal transcutaneous spinal direct current stimulation influences the amplitude of pain-related evoked potentials in healthy subjects
Authors: Eberhardt Frederic , Enax-Krumova Elena , Tegenthoff Martin , Höffken Oliver , Özgül Özüm Simal
Published: November 2023

It has already been described that transcutaneous spinal direct current stimulation (tsDCS) can selectively influence nociceptive evoked potentials. This study is the first aiming to prove an influence of tsDCS on pain-related evoked potentials (PREP) using concentric surface electrodes (CE), whose nociceptive specificity is still under discussion. 28 healthy subjects participated in this sham-controlled, double-blind cross-over study. All subjects underwent one session of anodal and one session of sham low-thoracic tsDCS. Before and after the intervention, PREP using CE, PREP-induced pain perception and somatosensory evoked potentials (SEP) were assessed on the right upper and lower limb. We found a decrease in PREP amplitude at the lower limb after sham stimulation, but not after anodal tsDCS, while SEP remained unchanged under all studied conditions. There was no difference between the effects of anodal tsDCS and sham stimulation on the studied parameters assessed at the upper limb. PREP-induced pain of the upper and lower limb increased after anodal tsDCS. The ability of influencing PREP using a CE at the spinal level in contrast to SEP suggests that PREP using CE follows the spinothalamic pathway and supports the assumption that it is specifically nociceptive. However, while mainly inhibitory effects on nociceptive stimuli have already been described, our results rather suggest that anodal tsDCS has a sensitizing effect. This may indicate that the mechanisms underlying the elicitation of PREP with CE are not the same as for the other nociceptive evoked potentials. The effects on the processing of different types of painful stimuli should be directly compared in future studies.
Exploiting nonlinearities through geometric engineering to enhance the auxetic behaviour in re-entrant honeycomb metamaterials
Authors: Srivastava Chetna , Bhola Lalit , Mahesh Vinyas , Guruprasad P. J. , Petrinic Nik , Scarpa Fabrizio , Harursampath Dineshkumar , Ponnusami Sathiskumar A.
Published: November 2023

Classical approaches to enhance auxeticity quite often involve exploring or designing newer architectures. In this work, simple geometrical features at the member level are engineered to exploit non-classical nonlinearities and improve the auxetic behaviour. The structural elements of the auxetic unit cell are here represented by thin strip-like beams, or thin-walled tubular beams. The resulting nonlinear stiffness enhances the auxeticity of the lattices, especially under large deformations. To quantify the influence of the proposed structural features on the resulting Poisson’s ratio, we use here variational asymptotic method (VAM) and geometrically exact beam theory. The numerical examples reveal that 2D re-entrant type micro-structures made of thin strips exhibit an improvement in terms of auxetic behaviour under compression. For the auxetic unit cell with thin circular tubes as members, Brazier’s effect associated with cross-sectional ovalisation improves the auxetic behaviour under tension; the enhancement is even more significant for the 3D re-entrant geometry. Thin strip-based auxetic unit cells were additively manufactured and tested under compression to verify the numerical observations. The experimentally measured values of the negative Poisson’s ratio are in close agreement with the numerical results, revealing a 66% increase due to the nonlinearity. Simulation results showcase these alternative approaches to improve the auxetic behaviour through simple geometric engineering of the lattice ribs.
Conditioned flight response in female rats to naturalistic threat is estrous-cycle dependent
Authors: Pyeon Gyeong Hee , Lee Jaeyong , Jo Yong Sang , Choi June-Seek
Published: November 2023

Despite the prevalent expression of freezing behavior following Pavlovian fear conditioning, a growing body of literature suggests potential sex differences in defensive responses. Our study investigated how female defensive behaviors are expressed in different threat situations and modulated by the estrous cycle. We aimed to compare freezing and flight-like responses during the acquisition and retrieval of fear conditioning using two distinct unconditioned stimuli (US) in two different spatial configurations: (1) electrical footshock (FUS) in a small, conventional enclosure with a grid floor, and (2) a predator-like robot (PUS) in a spacious, open arena. Fear conditioning with FUS showed no substantial differences between male and female rats of two different estrous cycles (proestrus and diestrus) in the levels of freezing and flight. However, when PUS was employed, proestrus female rats showed significantly more flight responses to the CS during both acquisition and the retrieval compared to the male and diestrus female rats. Taken together, our findings suggest that hormonal influences on the choice of defensive strategies in threat situations are significantly modulated by both the type of US and the spatial configuration of the environment.
Association of hypertension in pregnancy with serum electrolyte disorders in late pregnancy among Cameroonian women
Authors: Ajong Atem Bethel , Yakum Martin Ndinakie , Aljerf Loai , Ali Innocent Mbulli , Mangala Fulbert Nkwele , Onydinma Ukaogo Prince , Liwo Blaise Mbuomboh , Bekolo Cavin Epie , Tameh Theodore Yangsi , Kenfack Bruno , Telefo Phelix Bruno
Published: November 2023

Multiple electrolyte disorders, including sodium, potassium and calcium disorders, have been associated with hypertension in pregnancy. Most of these studies failed to evaluate the combined effect of low and high sodium, potassium, calcium and chloride ion concentrations on hypertension in pregnancy. This study evaluates the combined effect of these ion categories (low, normal, high) on hypertension in pregnancy. Biochemical ion assays and blood pressure measurements were carried out on 1074 apparently healthy pregnant women in late third trimester. Serum potassium, sodium, chloride, and ionised calcium were measured by ion-selective electrode potentiometry, while total plasma calcium was measured by absorption spectrophotometry. Hypertension in pregnancy was defined as systolic blood pressure ≥ 140 mmHg and/or diastolic blood pressure ≥ 90 mmHg. The prevalence of hyponatraemia, hypokalaemia, hypochloraemia, ionised hypocalcaemia and total hypocalcaemia in late pregnancy was 1.30 [0.78–2.18]%, 3.55 [2.60–4.84]%, 1.96 [1.28–2.97]%, 1.49 [0.92–2.21]% and 43.58 [40.64–46.56]%, respectively. Hypernatraemia, hyperkalaemia, hyperchloraemia, ionised hypercalcaemia and total hypercalcaemia were found in 1.49 [0.92–2.41]%, 2.34 [1.59–3.43]%, 4.38 [3.31–5.77]%, 39.94 [37.06–42.90]%, 2.79 [1.96–3.96]% of the participants, respectively. The prevalence of hypertension in pregnancy was 7.17 [5.77–8.87]%. When ion categories were considered in multiple logistic regression, only ionised and total calcium had significant associations with hypertension in pregnancy. Women with ionised hypercalcaemia had lower odds of hypertension in pregnancy (AOR = 0.50 [0.29–0.87], p-value = 0.015), and women with total hypocalcaemia had higher odds of hypertension in pregnancy (AOR = 1.99 [1.21–3.29], p-value = 0.007), compared to women with ionised and total normocalcaemia, respectively. Increasing kalaemia was associated significantly with higher odds of hypertension in pregnancy; however, kalaemia below and above the normal concentrations had no significant association with hypertension. Nonetheless, participants with kalaemia ≤ 3.98 mmol/L, had lower odds of hypertension in pregnancy compared with those with higher kalaemia (OR = 0.40 [0.24–0.66], p-value = 0.0003). Calcium disorders remain the most frequent electrolyte disorders in pregnancy. When normal cut-offs are considered for calcium and other ions, only ionised and total calcium influence the occurrence of hypertension in pregnancy. Kalaemia seems to affect hypertension in pregnancy but primarily within its normal concentrations. Serum electrolyte follow-up is indispensable for a proper pregnancy follow-up.
Development of a hybrid LSTM with chimp optimization algorithm for the pressure ventilator prediction
Authors: Ahmed Fatma Refaat , Alsenany Samira Ahmed , Abdelaliem Sally Mohammed Farghaly , Deif Mohanad A.
Published: November 2023

The utilization of mechanical ventilation is of utmost importance in the management of individuals afflicted with severe pulmonary conditions. During periods of a pandemic, it becomes imperative to build ventilators that possess the capability to autonomously adapt parameters over the course of treatment. In order to fulfil this requirement, a research investigation was undertaken with the aim of forecasting the magnitude of pressure applied on the patient by the ventilator. The aforementioned forecast was derived from a comprehensive analysis of many variables, including the ventilator's characteristics and the patient's medical state. This analysis was conducted utilizing a sophisticated computational model referred to as Long Short-Term Memory (LSTM). To enhance the predictive accuracy of the LSTM model, the researchers utilized the Chimp Optimization method (ChoA) method. The integration of LSTM and ChoA led to the development of the LSTM-ChoA model, which successfully tackled the issue of hyperparameter selection for the LSTM model. The experimental results revealed that the LSTM-ChoA model exhibited superior performance compared to alternative optimization algorithms, namely whale grey wolf optimizer (GWO), optimization algorithm (WOA), and particle swarm optimization (PSO). Additionally, the LSTM-ChoA model outperformed regression models, including K-nearest neighbor (KNN) Regressor, Random and Forest (RF) Regressor, and Support Vector Machine (SVM) Regressor, in accurately predicting ventilator pressure. The findings indicate that the suggested predictive model, LSTM-ChoA, demonstrates a reduced mean square error (MSE) value. Specifically, when comparing ChoA with GWO, the MSE fell by around 14.8%. Furthermore, when comparing ChoA with PSO and WOA, the MSE decreased by approximately 60%. Additionally, the analysis of variance (ANOVA) findings revealed that the p-value for the LSTM-ChoA model was 0.000, which is less than the predetermined significance level of 0.05. This indicates that the results of the LSTM-ChoA model are statistically significant.
Above-ground carbon stocks and its functional relationship with tree species diversity: the case of Kakamega and North Nandi Forests, Kenya
Authors: Obonyo Ouko Amose , Agevi Humphrey , Tsingalia Mugatsia Harrison
Published: November 2023

Estimating aboveground carbon (AGC) dynamics and tree diversity functionality relationships is critical in understanding the role of vegetation in implementing climate change mitigation strategies and promoting sustainable forest management. This study aimed to evaluate AGC stocks and their functional relationship with tree species diversity in Kakamega and North Nandi Forests, Kenya. A nested approach was adopted in sampling aboveground vegetation for biomass estimation in least disturbed, transformed, and disturbed sites. Tree biomass was estimated using an allometric equation based on tree diameter, tree height, and wood density. The biomass was then converted to carbon stocks using the carbon conversion factor. One-way ANOVA was used to determine the variation in carbon and tree diversity between forests and forest types. The correlation between tree diversity and AGC was evaluated. It was established that Kakamega Forest had the highest AGC (157.93 ± 26.91tha−1). The least disturbed areas had the highest AGC (65.96 ± 8.56tha−1). Additionally, Shannon diversity revealed a higher tree species diversity in Kakamega Forest (H′ = 1.82 ± 0.95). There was a significant positive correlation between AGC and tree species diversity (r = 0.62, p < 0.05). Kakamega and North Nandi forests vary in their AGC, and that tree species diversity positively influences the AGC of the two forests.
Examining disparities in cardiovascular disease prevention strategies and incidence rates between urban and rural populations: insights from Kazakhstan
Authors: Glushkova Natalya , Turdaliyeva Botagoz , Kulzhanov Maksut , Karibayeva Indira K. , Kamaliev Maksut , Smailova Dariga , Zhamakurova Ayaulym , Namazbayeva Zhanar , Mukasheva Gulmira , Kuanyshkalieva Asylzhan , Otyzbayeva Nurzhamal , Semenova Yuliya , Jobalayeva Bagym
Published: November 2023

Kazakhstan is experiencing a high burden of cardiovascular disease (CVD), and the country has implemented a range of strategies aimed at controlling CVD. The study aims to conduct a content analysis of the policies implemented in the country and augment it with an analysis of official statistics over a 15-year period, from 2006 to 2020. The study also includes comparisons of incidence rates between urban and rural areas. A comprehensive search was conducted to identify policy documents that regulate the provision of primary, secondary, and tertiary prevention of cardiovascular diseases. Additionally, official data on the incidence of arterial hypertension, ischemic heart disease, acute myocardial infarction, and cerebrovascular disease were extracted from official statistics, disaggregated by urban and rural areas. Forecast modeling was utilized to project disease incidences up to 2030. The study reveals that Kazakhstan primarily focuses on tertiary prevention of cardiovascular diseases, with less attention given to secondary prevention, and primary prevention is virtually non-existent. In general, screening for arterial hypertension appears to be more successful than for ischemic heart disease. The incidence of arterial hypertension has increased threefold for urban residents and 1.7-fold for rural residents. In urban areas, residents saw a twofold increase in ischemic heart disease incidence, while it remained the same in rural areas. The findings of this study have practical implications for decision-makers, who can use the results to enhance the effectiveness of existing CVD prevention strategies.
Coexistent ARID1A-PIK3CA mutations are associated with immune-related pathways in luminal breast cancer
Authors: Anabel Sinberger Liat , Zahavi Tamar , Sonnenblick Amir , Salmon‐Divon Mali
Published: November 2023

Up to 40% of luminal breast cancer patients carry activating mutations in the PIK3CA gene. PIK3CA mutations commonly co-occur with other mutations, but the implication of this co-occurrence may vary according to the specific genes involved. Here, we characterized a subgroup of luminal breast cancer expressing co-mutations in ARID1A and PIK3CA genes and identified their effect on important signaling pathways. Our study included 2609 primary breast cancer samples from the TCGA and METABRIC datasets that were classified based on tumor subtype and the existence of mutations in PIK3CA and ARID1A genes. Differential expression and WGCNA analyses were performed to detect molecular modules affected by the existence of the mutations. Our results reveal various evidence for the involvement of immune-related pathways in luminal tumors harboring ARID1A and PIK3CA mutations, as well as a unique Tumor-infiltrated immune cells composition. We also identified seven key hub genes in the ARID1A-PIK3CA mutated tumors associated with immune-related pathways: CTLA4, PRF1, LCK, CD3E, CD247, ZAP70, and LCP2. Collectively, these results indicate an immune system function that may contribute to tumor survival. Our data induced a hypothesis that ARID1A and PIK3CA mutations' co-occurrence might predict responses to immunotherapy in luminal BC and, if validated, could guide immunotherapy development.
Maternal early mid-pregnancy adiponectin in relation to infant birth weight and the likelihood of being born large-for-gestational-age
Authors: Lindberger Emelie , Larsson Anders , Kunovac Kallak Theodora , Sundström Poromaa Inger , Wikström Anna-Karin , Österroos Anna , Ahlsson Fredrik
Published: November 2023

This study aimed to evaluate the association of maternal adiponectin with infant birth size in 1349 pregnant women at Uppsala University Hospital, Sweden. The mean age of the women was 31.0 years, and 40.9% were nulliparous. Maternal early mid-pregnancy adiponectin was measured in microgram/mL. Linear regression models were performed to evaluate the association between adiponectin and infant birth weight. Logistic regression models were used to evaluate adiponectin in relation to the odds of giving birth to an infant large-for-gestational-age (LGA, infant birth weight standard deviation score > 90th percentile). Adjustments were made for early pregnancy BMI and diabetes mellitus. Prior adjustments, adiponectin was inversely associated with infant birth weight (β − 17.1, 95% confidence interval (CI) − 26.8 to − 7.4 g, P < 0.001), and one microgram/mL increase in adiponectin was associated with a 9% decrease in the odds of giving birth to an LGA infant (odds ratio 0.91, CI 0.85–0.97, P = 0.006). The associations did not withstand in the adjusted models. We found a significant interaction between adiponectin and infant sex on birth size. This interaction was driven by an inverse association between maternal adiponectin and birth size in female infants, whereas no such association was found in males.
Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants
Authors: Shen Yunxin , Shi Zhufeng , Zhao Jiangyuan , Li Minggang , Tang Jiacai , Wang Nan , Mo Yanfang , Yang Tongyu , Zhou Xudong , Chen Qibin , Yang Peiweng
Published: November 2023

Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. SH-1471 contains a ring chromosome of 4,181,346 bp with a mean G + C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. We also found that there are many metabolites in the strain that can inhibit the growth of pathogens. In addition, our indoor pot test found that SH-1471 has a good control effect on tomato wilt, and could significantly improve plant height, stem circumference, root length, root weight, and fresh weight and dry weight of the aboveground part of tomato seedlings. Therefore, SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of SH-1471 in biological control of plant diseases and promote its application.
Comparative analysis of endophytic fungal communities in bamboo species Phyllostachys edulis, Bambusa rigida, and Pleioblastus amarus
Authors: Yan Kuan , Zhang Jian , Cai Yu , Cao Guiling , Meng Lina , Soaud Salma A. , Heakel Rania M. Y. , Ihtisham Muhammad , Zhao Xianming , Wei Qin , Dai Tainfei , Abbas Manzar , El-Sappah Ahmed H.
Published: November 2023

Fungal endophytes in plant leaf mesophyll form mutually beneficial associations through carbon assimilation, synthesis of biologically active chemicals, and enhancement of aesthetic and nutritional value. Here, we compared community structure, diversity, and richness of endophytic fungi in the leaves of three bamboo species, including Phyllostachys edulis (MZ), Bambusa rigida (KZ), and Pleioblastus amarus (YT) via high-throughput Illumina sequencing. In total, 1070 operational taxonomic units (OTUs) were retrieved and classified into 7 phylum, 27 classes, 82 orders, 185 families, 310 genus, and 448 species. Dominant genera were Cladosporium, Trichomerium, Hannaella, Ascomycota, Sporobolomyces, Camptophora and Strelitziana. The highest fungal diversity was observed in Pleioblastus amarus, followed by Bambusa rigida, and Phyllostachys edulis. Comparatively, monopodial species Ph. edulis and sympodial B. rigida, mixed P. amarus revealed the highest richness of endophytic fungi. We retrieved a few biocontrol agents, Sarocladium and Paraconiothyrium, and unique Sporobolomyces, Camptophora, and Strelitziana genera. FUNGuild analysis revealed the surrounding environment (The annual average temperature is between 15 and 25 °C, and the relative humidity of the air is above 83% all year round) as a source of fungal accumulation in bamboo leaves and their pathogenic nature. Our results provide precise knowledge for better managing bamboo forests and pave the way for isolating secondary metabolites and potential bioactive compounds.
Single cell and spatial transcriptomics analysis of kidney double negative T lymphocytes in normal and ischemic mouse kidneys
Authors: Gharaie Sepideh , Lee Kyungho , Noller Kathleen , Lo Emily K. , Miller Brendan , Jung Hyun Jun , Newman-Rivera Andrea M. , Kurzhagen Johanna T. , Singla Nirmish , Welling Paul A. , Fan Jean , Cahan Patrick , Noel Sanjeev , Rabb Hamid
Published: November 2023

T cells are important in the pathogenesis of acute kidney injury (AKI), and TCR+CD4-CD8- (double negative-DN) are T cells that have regulatory properties. However, there is limited information on DN T cells compared to traditional CD4+ and CD8+ cells. To elucidate the molecular signature and spatial dynamics of DN T cells during AKI, we performed single-cell RNA sequencing (scRNA-seq) on sorted murine DN, CD4+, and CD8+ cells combined with spatial transcriptomic profiling of normal and post AKI mouse kidneys. scRNA-seq revealed distinct transcriptional profiles for DN, CD4+, and CD8+ T cells of mouse kidneys with enrichment of Kcnq5, Klrb1c, Fcer1g, and Klre1 expression in DN T cells compared to CD4+ and CD8+ T cells in normal kidney tissue. We validated the expression of these four genes in mouse kidney DN, CD4+ and CD8+ T cells using RT-PCR and Kcnq5, Klrb1, and Fcer1g genes with the NIH human kidney precision medicine project (KPMP). Spatial transcriptomics in normal and ischemic mouse kidney tissue showed a localized cluster of T cells in the outer medulla expressing DN T cell genes including Fcer1g. These results provide a template for future studies in DN T as well as CD4+ and CD8+ cells in normal and diseased kidneys.
Semi-empirical parameterization of HI/p L-shell X-ray production cross section ratios in Bi for Heavy Ion PIXE
Authors: Masekane M. C. , Msimanga M. , Bogdanović Radović I. , Madhuku M. , Moloi S. J.
Published: November 2023

Quantitative analysis of materials from Heavy Ion PIXE spectra remains impeded by the lack of reliable X-ray production cross section (XPCS) data. Although efforts at experimental Heavy Ion induced XPCS measurements still continue, Multiple Ionisation (MI) effects, which are not fully described by theory, render simulations of heavy ion PIXE data unreliable for large Z1/Z2 collisions, especially at low energies. This is also exacerbated by the random selection of projectile-target combinations for measured and reported experimental data available to validate theory. This study explored heavy ion induced X-ray production cross section deviations from those induced by protons at the same ion velocity. This enabled evaluations of the degree to which cross sections are enhanced through MI effects, with the aim of predicting XPCS due to heavy ion impact. The evaluation was carried out through the scaling of experimental heavy ion to theoretical proton cross section ratios (R), which were then used for the interpolation of XPCS in the same target element for ‘missing’ projectiles within the range of evaluation. Here we present measurements of heavy ion induced total L-shell XPCS in Bi, carried out to determine HI/p MI induced deviations due to C, F, Cl and Ti projectiles at an ion velocity range of (0.2–1.0) MeV/nucleon.
Factors affecting social phobia among Chinese college students in the context of COVID-19 pandemic: a cross-sectional study
Authors: Lin Hai , Yang Ziming , Huo Shanshan , Su Caixia , Zhang Zhongsong , Rao Yingting , Yin Hui
Published: November 2023

Social phobia (SP) refers to excessive anxiety about social interactions. College students, with their exposure to academic, familial, and job-related pressures, are an ideal population for early screening and intervention of social phobia. Additionally, COVID-19 prevention measures including keeping social distance may further impact social phobia. This study aims to investigate the influencing factors of social phobia among Chinese college students and to tentatively explore the impact of COVID-19 prevention measures on social phobia. Respondents were recruited through Chinese Internet social platforms for an online survey. College students’ social phobia scores in pre- and early-COVID-19 periods were measured using Peters' short form of the Social Interaction Anxiety Scale and Social Phobia Scale (SIAS-6/SPS-6). Demographic information, family information, social relations, self-evaluation, and subjective feelings regarding the impact of COVID-19 preventive measures on social phobia were collected. A multivariable logistic regression model was used to analyze the influencing factors. A total of 1859 valid questionnaires were collected, revealing that the social phobia scores increased from 12.3 ± 11.9 to 13.4 ± 11.9 between pre- and early-COVID-19 periods, with an increase of 1.0 ± 6.4 (p < 0.001). Low GPA rank, mobile phone dependence, distant family relationships, indulgent parents, childhood adversity, and childhood bullying were risk factors for social phobia among Chinese college students. Female gender, being a senior university student or postgraduate, satisfaction with physical appearance, self-reported good mental health and high level of interpersonal trust were protective factors for social phobia. Although most respondents believed that COVID-19 prevention measures (e.g., mask wearing and social distancing rules) reduced their social phobia, these measures were not significantly associated with social phobia levels in the multivariable analyses. In conclusion, Chinese college students’ social phobia was widely influenced by diverse factors and warrants increased attention, with early intervention aimed at high-risk individuals being crucial for their mental health. Additional research is necessary to understand the impact of COVID-19 preventive measures on social phobia among college students.
Identification and characterization of ATM founder mutation in BRCA-negative breast cancer patients of Arab ethnicity
Authors: Bu Rong , Siraj Abdul K. , Al-Rasheed Maha , Iqbal Kaleem , Azam Saud , Qadri Zeeshan , Haqawi Wael , Tulbah Asma , Al-Dayel Fouad , Almalik Osama , Al-Kuraya Khawla S.
Published: November 2023

Breast cancer (BC) is the most prevalent malignancy among women worldwide with germline pathogenic variants/likely pathogenic variants (PVs/LPVs) in BRCA1/2 accounting for a large portion of hereditary cases. Recently, heterozygous PVs/LPVs in the ATM serine/threonine kinase or Ataxia-telangiectasia mutated gene (ATM) has been identified as a moderate susceptibility factor for BC in diverse ethnicities. However, the prevalence of ATM PVs/LPVs in BC susceptibility in Arab populations remains largely unexplored. This study investigated the prevalence of ATM PVs/LPVs among BC patients from Saudi Arabia, employing capture-sequencing technology for ATM PVs/LPVs screening in a cohort of 715 unselected BC patients without BRCA1/2 PVs/LPVs. In addition, founder mutation analysis was conducted using the PHASE program. In our entire cohort, four unique PVs/LPVs in the ATM gene were identified in six cases (0.8%). Notably, one recurrent LPV, c.6115G > A:p.Glu2039Lys was identified in three cases, for which haplotype analysis confirmed as a novel putative founder mutation traced back to 13 generations on average. This founder mutation accounted for half of all identified mutant cases and 0.4% of total screened cases. This study further reveals a significant correlation between the presence of ATM mutation and family history of BC (p = 0.0127). These findings underscore an approximate 0.8% prevalence of ATM germline PVs/LPVs in Arab BC patients without BRCA1/2 PVs/LPVs and suggest a founder effect of specific recurrent ATM mutation. These insights can help in the design of a genetic testing strategy tailored to the local population in Saudi Arabia, thereby, enabling more accurate clinical management and risk prediction.
Multi-source transfer learning for facial emotion recognition using multivariate correlation analysis
Authors: B Ashwini , Sarkar Arka , Behera Pruthivi Raj , Shukla Jainendra
Published: November 2023

Deep learning techniques have proven to be effective in solving the facial emotion recognition (FER) problem. However, it demands a significant amount of supervision data which is often unavailable due to privacy and ethical concerns. In this paper, we present a novel approach for addressing the FER problem using multi-source transfer learning. The proposed method leverages the knowledge from multiple data sources of similar domains to inform the model on a related task. The approach involves the optimization of aggregate multivariate correlation among the source tasks trained on the source dataset, thus controlling the transfer of information to the target task. The hypothesis is validated on benchmark datasets for facial emotion recognition and image classification tasks, and the results demonstrate the effectiveness of the proposed method in capturing the group correlation among features, as well as being robust to negative transfer and performing well in few-shot multi-source adaptation. With respect to the state-of-the-art methods MCW and DECISION, our approach shows an improvement of 7% and $$\sim$$
∼
15% respectively.
Vertical dynamic visual acuity is significantly lower than horizontal dynamic visual acuity
Authors: Tachihara Aoi , Soh Zu , Mizuguchi Tomohiko , Kandori Akihiko , Hama Seiji , Tsuji Toshio
Published: November 2023

Dynamic visual acuity (DVA) is crucial for the perception of moving objects. While traditional DVA assessment tools predominantly focus on horizontal movements, the evaluation of vertical DVA remains unstandardized. Consequently, the disparities between vertical and horizontal DVAs are yet to be thoroughly investigated. Therefore, we designed a system capable of conducting multidirectional DVA tests and eye movement measurements. During the experiments, the participants identified the gap direction of the Landolt-C ring moving either horizontally or vertically. The speed of movement decelerated from its maximum as a high-speed infrared camera captured the pupil movements of the left eye at 500 fps. We conducted tests on 15 healthy university students (aged $$22.7\pm 1.2$$

22.7
±
1.2

years) and measured vertical and horizontal DVAs five times each. DVA was deduced from the Landolt-C ring speed with accurate gap direction responses, and eye movement was assessed based on the total gaze movement distance. The results revealed superior DVA and eye movement in the horizontal direction compared with the vertical direction ($$p < 0.001$$

p
<
0.001

). This highlights the anisotropic characteristics of DVA and eye movement. The proposed system has the potential for multidirectional dynamic vision evaluation and training in clinical scenarios.