bluuebunny/crossref_metadata_2025 huggingface dataset:
Epstein-Barr Virus Positive B-Cell Lymphoproliferative Disorder of the Gastrointestinal Tract
Authors: Ishikawa Eri , Satou Akira , Nakamura Masanao , Nakamura Shigeo , Fujishiro Mitsuhiro
Published: July 2021

Epstein-Barr virus positive B-cell lymphoproliferative disorder (EBV+ B-LPD) encompasses a broad clinicopathological spectrum and distinct clinical behavior that relatively favors the gastrointestinal (GI) tract. In this review, we provide an update on the clinicopathological features and biological behavior of EBV-positive mucocutaneous ulcer (EBVMCU) and primary EBV+ diffuse large B-cell lymphoma (DLBCL) of the GI tract. EBVMCU is a newly recognized entity but well known as an indolent and self-limited EBV+ B-LPD occurring in various immunodeficiencies. In contrast, EBV+ DLBCL constitutes the largest group of EBV+ B-LPDs and is regarded as an aggressive neoplasm. These two distinct diseases have historically been distinguished in the reappraisal of age-related EBV-associated B-LPDs but are challenging in routine practice regarding their differential diagnostic and therapeutic approaches. An increasing number of reports indicate that they are epidemiologically prevalent beyond western and eastern countries, but their comprehensive analysis is still limited. We also describe the PD-L1 positivity of tumorous large cells and non-malignant immune cells, which is relevant for the prognostic delineation among patients with primary DLBCL of the GI tract with and without EBV on tumor cells.
Immunohistochemical Evaluation of FGD3 Expression: A New Strong Prognostic Factor in Invasive Breast Cancer
Authors: Susini Tommaso , Saccardin Giulia , Renda Irene , Giani Milo , Tartarotti Enrico , Nori Jacopo , Vanzi Ermanno , Pasqualini Elisa , Bianchi Simonetta
Published: July 2021

Among new prognostic factors for breast cancer, the most promising one seems to be FGD3 (Facio-Genital Dysplasia 3) gene, whose expression improves outcome by inhibiting cell migration. The aim of the study was to evaluate the prognostic role of FGD3 in invasive breast cancer in a series of 401 women, treated at our unit, by evaluating the expression of this gene by immunohistochemistry. Patients with high FGD3 expression showed a significantly better disease-free survival (DFS) (p < 0.001) and overall survival (OS) (p < 0.001). The prognostic value of FGD3 expression was stronger than that of classical pathologic parameters such as histological grade of differentiation, Ki-67 index and molecular subtype. By multivariate Cox analysis, FGD3 expression was confirmed as significant and independent prognostic factor, ranking second after age at diagnosis (≤40 years) for DFS (p = 0.003) and the second strongest predictor of OS, after AJCC Stage (p < 0.001). Our data suggest that inclusion of FGD3 evaluation in the routine workup of breast cancer patients may result in a more accurate stratification of the individual risk. The possibility to assess FGD3 expression by a simple and cheap technique such as immunohistochemistry may enhance the spread of its use in the clinical practice.
Characteristics and Clinical Application of Extracellular Vesicle-Derived DNA
Authors: Hur Jae Young , Lee Kye Young
Published: July 2021

Extracellular vesicles (EVs) carry RNA, proteins, lipids, and diverse biomolecules for intercellular communication. Recent studies have reported that EVs contain double-stranded DNA (dsDNA) and oncogenic mutant DNA. The advantage of EV-derived DNA (EV DNA) over cell-free DNA (cfDNA) is the stability achieved through the encapsulation in the lipid bilayer of EVs, which protects EV DNA from degradation by external factors. The existence of DNA and its stability make EVs a useful source of biomarkers. However, fundamental research on EV DNA remains limited, and many aspects of EV DNA are poorly understood. This review examines the known characteristics of EV DNA, biogenesis of DNA-containing EVs, methylation, and next-generation sequencing (NGS) analysis using EV DNA for biomarker detection. On the basis of this knowledge, this review explores how EV DNA can be incorporated into diagnosis and prognosis in clinical settings, as well as gene transfer of EV DNA and its therapeutic potential.
Biomarkers and Gene Signatures to Predict Durable Response to Pembrolizumab in Non-Small Cell Lung Cancer
Authors: Poma Anello Marcello , Bruno Rossella , Pietrini Iacopo , Alì Greta , Pasquini Giulia , Proietti Agnese , Vasile Enrico , Cappelli Sabrina , Chella Antonio , Fontanini Gabriella
Published: July 2021

Pembrolizumab has been approved as first-line treatment for advanced Non-small cell lung cancer (NSCLC) patients with tumors expressing PD-L1 and in the absence of other targetable alterations. However, not all patients that meet these criteria have a durable benefit. In this monocentric study, we aimed at refining the selection of patients based on the expression of immune genes. Forty-six consecutive advanced NSCLC patients treated with pembrolizumab in first-line setting were enrolled. The expression levels of 770 genes involved in the regulation of the immune system was analysed by the nanoString system. PD-L1 expression was evaluated by immunohistochemistry. Patients with durable clinical benefit had a greater infiltration of cytotoxic cells, exhausted CD8, B-cells, CD45, T-cells, CD8 T-cells and NK cells. Immune cell scores such as CD8 T-cell and NK cell were good predictors of durable response with an AUC of 0.82. Among the immune cell markers, XCL1/2 showed the better performance in predicting durable benefit to pembrolizumab, with an AUC of 0.85. Additionally, CD8A, CD8B and EOMES showed a high specificity (>0.86) in identifying patients with a good response to treatment. In the same series, PD-L1 expression levels had an AUC of 0.61. The characterization of tumor microenvironment, even with the use of single markers, can improve patients’ selection for pembrolizumab treatment.
A Novel Predictive Tool for Discriminating Endometriosis Associated Ovarian Cancer from Ovarian Endometrioma: The R2 Predictive Index
Authors: Kawahara Naoki , Miyake Ryuta , Yamanaka Shoichiro , Kobayashi Hiroshi
Published: July 2021

Background: Magnetic resonance (MR) relaxometry provides a noninvasive tool to discriminate between ovarian endometrioma (OE) and endometriosis-associated ovarian cancer (EAOC), with a sensitivity and specificity of 86% and 94%, respectively. MRI models that can measure R2 values are limited, and the R2 values differ between MRI models. This study aims to extract the factors contributing to the R2 value, and to make a formula for estimating the R2 values, and to assess whether the R2 predictive index calculated by the formula could discriminate EAOC from OE. Methods: This retrospective study was conducted at our institution from November 2012 to February 2019. A total of 247 patients were included in this study. Patients with benign ovarian tumors mainly received laparoscopic surgery, and the patients suspected of having malignant tumors underwent laparotomy. Information from a chart review of the patients’ medical records was collected. Results: In the investigative cohort, among potential factors correlated with the R2 value, multiple regression analyses revealed that tumor diameter and CEA could predict the R2 value. In the validation cohort, multivariate analysis confirmed that age, CRP, and the R2 predictive index were the independent factors. Conclusions: The R2 predictive index is useful and valuable to the detection of the malignant transformation of endometrioma.
Drosophila Models Rediscovered with Super-Resolution Microscopy
Authors: Szikora Szilárd , Görög Péter , Kozma Csaba , Mihály József
Published: July 2021

With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in Drosophila model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in Drosophila in understanding fundamental subcellular constituents.
Acute RhoA/Rho Kinase Inhibition Is Sufficient to Restore Phagocytic Capacity to Retinal Pigment Epithelium Lacking the Engulfment Receptor MerTK
Authors: Mao Yingyu , Finnemann Silvia C.
Published: July 2021

The diurnal phagocytosis of spent photoreceptor outer segment fragments (POS) by retinal pigment epithelial (RPE) cells is essential for visual function. POS internalization by RPE cells requires the assembly of F-actin phagocytic cups beneath surface-tethered POS and Mer tyrosine kinase (MerTK) signaling. The activation of the Rho family GTPase Rac1 is necessary for phagocytic cup formation, and Rac1 is activated normally in MerTK-deficient RPE. We show here that mutant RPE lacking MerTK and wild-type RPE deprived of MerTK ligand both fail to form phagocytic cups regardless of Rac1 activation. However, in wild-type RPE in vivo, a decrease in RhoA activity coincides with the daily phagocytosis burst, while RhoA activity in MerTK-deficient RPE is constant. Elevating RhoA activity blocks phagocytic cup formation and phagocytosis by wild-type RPE. Conversely, inhibiting RhoA effector Rho kinases (ROCKs) rescues both F-actin assembly and POS internalization of primary RPE if MerTK or its ligand are lacking. Most strikingly, acute ROCK inhibition is sufficient to induce the formation and acidification of endogenous POS phagosomes by MerTK-deficient RPE ex vivo. Altogether, RhoA pathway inactivation is a necessary and sufficient downstream effect of MerTK phagocytic signaling such that the acute manipulation of cytosolic ROCK activity suffices to restore phagocytic capacity to MerTK-deficient RPE.
Neuroinflammation and Alzheimer’s Disease: A Machine Learning Approach to CSF Proteomics
Authors: Gaetani Lorenzo , Bellomo Giovanni , Parnetti Lucilla , Blennow Kaj , Zetterberg Henrik , Di Filippo Massimiliano
Published: July 2021

In Alzheimer’s disease (AD), the contribution of pathophysiological mechanisms other than amyloidosis and tauopathy is now widely recognized, although not clearly quantifiable by means of fluid biomarkers. We aimed to identify quantifiable protein biomarkers reflecting neuroinflammation in AD using multiplex proximity extension assay (PEA) testing. Cerebrospinal fluid (CSF) samples from patients with mild cognitive impairment due to AD (AD-MCI) and from controls, i.e., patients with other neurological diseases (OND), were analyzed with the Olink Inflammation PEA biomarker panel. A machine-learning approach was then used to identify biomarkers discriminating AD-MCI (n: 34) from OND (n: 25). On univariate analysis, SIRT2, HGF, MMP-10, and CXCL5 showed high discriminatory performance (AUC 0.809, p = 5.2 × 10−4, AUC 0.802, p = 6.4 × 10−4, AUC 0.793, p = 3.2 × 10−3, AUC 0.761, p = 2.3 × 10−3, respectively), with higher CSF levels in AD-MCI patients as compared to controls. These same proteins were the best contributors to the penalized logistic regression model discriminating AD-MCI from controls (AUC of the model 0.906, p = 2.97 × 10−7). The biological processes regulated by these proteins include astrocyte and microglia activation, amyloid, and tau misfolding modulation, and blood-brain barrier dysfunction. Using a high-throughput multiplex CSF analysis coupled with a machine-learning statistical approach, we identified novel biomarkers reflecting neuroinflammation in AD. Studies confirming these results by means of different assays are needed to validate PEA as a multiplex technique for CSF analysis and biomarker discovery in the field of neurological diseases.
The Role of the Nurse in Informed Consent to Treatments: An Observational-Descriptive Study in the Padua Hospital
Authors: Strini Veronica , Schiavolin Roberta , Prendin Angela
Published: July 2021

Background: The process to obtain valid informed consent in healthcare reflects many aspects. Healthcare professionals that take care of the patient must provide him all the necessary information and verify his understanding, considering individual characteristics. Nurses are one of the main participants in this process. Objective: This study assesses nurses’ perceptions of their role in the informed consent process. Material and Methods: An observational study involving 300 nurses operating in 13 wards of the Padua Hospital, through the submitting of a questionnaire in the period November–December 2018. Results: The final sample is made up of 206 nurses—27 males (13.11%) and 179 females (86.89%). Work experience, on average 15 years, is significant in determining the answers to questions about opinions and experiences. Age is significant in determining how often nurses provide information to the patient’s family members about the actions to be taken after discharge. The ward was decisive in the responses related to information provided to patients on the nursing care level and the actions to be taken after discharge, and the definition of the nurse’s duties. Conclusions: The data collected show the need for interventions to reduce the causes of difficult that the nurse has in informing patients.
Fabrication of a Novel MgO-B2O3-SiO2-Zn Coating by Thermal Spraying
Authors: Yu Zhan , Song Bo , Ma Ping , Fan Wenhui , Gong Enzhong , Sun Yilun , Ding Yunlong , Ju Dongying
Published: July 2021

In automotive technology, the proper use of thermal insulation materials helps improve the performance and life of internal equipment and reduce maintenance cost. In this study, plasma spraying and flame spraying are used to prepare and coat a new MgO-B2O3-SiO2-Zn powder on the SUS304 substrate. The resulting coating as thermal insulation layer formed a networked microstructure between the substrates to improve the thermal insulation performance of the material. By validation of thermal radiation experiments, the thermal insulation effects of various ceramic powders were compared, the high-temperature and low-temperature thermal insulation materials for about 300 and 100 °C were determined, and the thermal insulation performance of the constructed material coatings was verified by analysis.
Study of Black Silicon Wafer through Wet Chemical Etching for Parametric Optimization in Enhancing Solar Cell Performance by PC1D Numerical Simulation
Authors: Arafat Md. Yasir , Islam Mohammad Aminul , Mahmood Ahmad Wafi Bin , Abdullah Fairuz , Kiong Tiong Sieh , Amin Nowshad
Published: July 2021

Black silicon (BSi) fabrication via surface texturization of Si-wafer in recent times has become an attractive concept regarding photon trapping and improved light absorption properties for photovoltaic applications. In this study, surface texturization has been conducted on mono-crystalline Si(100) wafer using a wet chemical anisotropic etching process with IPA:KOH solution to form micro-pyramidal surface structures. Moreover, the optimized properties of the fabricated BSi wafers are used for numerical simulation using PC1D software to analyze the performance of the solar cell and establish the correlation among relevant parameters. Effects such as doping concentration, texturization, passivation, and anti-reflection coating of BSi on the solar cell performance have numerically been investigated. Results show that textured surface obtained from the wet chemical anisotropic etching process has successfully reduced the reflectance of the BSi wafer and surpassed the solar cell efficiency by 2%, which is mainly attributed to the optical confinement of the textured pyramids on the surface with a height of 1–2 μm and angles of 70 degrees. Furthermore, the doping concentration of the p-type wafer and n-type emitter were optimized to be 1 × 1016 cm−3 and 1 × 1018 cm−3, respectively. In the case of device optimization, the SiO2 passivation layer with a refractive index of 1.48 and the Si3N4 ARC layer with a refractive index of 2.015 has been identified as the best combination for the solar cell performance. These optimized parameters eventually result in 23.14% conversion efficiency from numerical simulation for solar cells that use black silicon wafers as fabricated in this study.
A Novel Method for the Preparation of Fibrous CeO2–ZrO2–Y2O3 Compacts for Thermochemical Cycles
Authors: Knoblauch Nicole , Mechnich Peter
Published: July 2021

Zirconium-Yttrium-co-doped ceria (Ce0.85Zr0.13Y0.02O1.99) compacts consisting of fibers with diameters in the range of 8–10 µm have been successfully prepared by direct infiltration of commercial YSZ fibers with a cerium oxide matrix and subsequent sintering. The resulting chemically homogeneous fiber-compacts are sinter-resistant up to 1923 K and retain a high porosity of around 58 vol% and a permeability of 1.6–3.3 × 10−10 m² at a pressure gradient of 100–500 kPa. The fiber-compacts show a high potential for the application in thermochemical redox cycling due its fast redox kinetics. The first evaluation of redox kinetics shows that the relaxation time of oxidation is five times faster than that of dense samples of the same composition. The improved gas exchange due to the high porosity also allows higher reduction rates, which enable higher hydrogen yields in thermochemical water-splitting redox cycles. The presented cost-effective fiber-compact preparation method is considered very promising for manufacturing large-scale functional components for solar-thermal high-temperature reactors.
Efficacy of Oral Cryotherapy in the Prevention of Oral Mucositis Associated with Cancer Chemotherapy: Systematic Review with Meta-Analysis and Trial Sequential Analysis
Authors: Al-Rudayni Ali Hatem Manfi , Gopinath Divya , Maharajan Mari Kannan , Veettil Sajesh Kalkandi , Menon Rohit Kunnath
Published: July 2021

Background: This review aimed to evaluate the efficacy of oral cryotherapy in the prevention of chemotherapy-induced oral mucositis using meta-analysis and trial sequential analysis, as well as to assess the quality of the results by the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Methods: A comprehensive search of three databases including Medline, Embase and Central was performed to identify randomized controlled trials that used oral cryotherapy for the prevention of chemotherapy-induced oral mucositis. The primary outcome was the incidence of oral mucositis for trials employing oral cryotherapy as the intervention for the prevention of oral mucositis. The meta-analysis was performed using the random-effects model and random errors of the meta-analyses were detected by trial sequential analysis. Results: A total of 14 RCTs with 1577 participants were included in the present meta-analysis. Patients treated with oral cryotherapy were associated with a significantly lower risk of developing oral mucositis of any grade (risk ratio (RR), 0.67 (95% CI: 0.56–0.81, p < 0.05)). Findings from the subgroup analyses showed that oral cryotherapy significantly reduced the risk of oral mucositis in patients undergoing bone marrow transplantation (RR 0.69, CI: 0.54–0.89, p < 0.05) as well as chemotherapy (RR 0.66, CI: 0.58–0.75, p < 0.05). Findings from the trial sequential analysis suggested that the evidence on oral cryotherapy as a preventive intervention for oral mucositis in patients with solid malignancies receiving conventional chemotherapy was conclusive. Conclusion: Oral cryotherapy is effective in preventing oral mucositis in patients undergoing chemotherapy for the management of solid malignancies. The use of oral cryotherapy in preventing oral mucositis in bone marrow transplantation settings showed promising efficacy, but the evidence is not conclusive and requires more high-quality randomized controlled trials.
3q26 Amplifications in Cervical Squamous Carcinomas
Authors: Voutsadakis Ioannis A.
Published: July 2021

Background: Squamous carcinomas of the uterine cervix often carry mutations of the gene encoding for the catalytic sub-unit of kinase PI3K, PIK3CA. The locus of this gene at chromosome 3q26 and neighboring loci are also commonly amplified. The landscape of 3q26-amplified cases have not been previously characterized in detail in cervical cancer. Methods: Published genomic data and associated clinical data from TCGA cervical cancer cohort were analyzed at cBioportal for amplifications in genes at 3q26. The clinical and molecular characteristics of the group of patients with 3q26 amplifications was compared with the group without 3q26 amplifications. Comparative prevalence of amplification and expression of genes at 3q26 in amplified squamous cervical cancer cases were surveyed as well as 3q26 amplifications in cervical cancer cell line databases. Results: Amplification of 3q26 locus is a prevalent molecular lesion in cervical squamous cell carcinomas encountered in about 15% of cases in TCGA cohort of 247 patients. Cancer-related genes commonly amplified from 3q26 include PIK3CA, TBL1XR1, DCUN1D1, SOX2, MECOM, PRKCI, and TERC. Amplified cases do not completely overlap with PIK3CA mutant cases. Differences exist between 3q26-amplified and non-amplified carcinomas in the frequency of mutations and frequency of other amplifications. Most commonly over-expressed genes in 3q26 amplified cases include PIK3CA, TBL1XR1, DCUN1D1, and less commonly SOX2 and PRKCI. Conclusion: The subset of squamous cervical carcinomas with 3q26 amplifications is not overlapping with cancers carrying PIK3CA mutations and contains, besides PIK3CA, other cancer-associated genes that are over-expressed at the mRNA level, including TBL1XR1 and DCUN1D1. DCUN1D1, a regulator of SCF ubiquitin ligase activity, may be a relevant pathogenic player given the importance of ubiquitination and the proteasome in the disease. These observations could form the basis for therapeutic exploitation in this subset of squamous cervical carcinomas.
GIST and Ghrelin: To Be or Not to Be?
Authors: Spiridon Irene Alexandra , Ciobanu Delia Gabriela Apostol , Giușcă Simona Eliza , Ferariu Dan , Pleşca Iulia Cătălina , Căruntu Irina Draga
Published: July 2021

Background: Ghrelin is the orexigenic hormone secreted mainly by the stomach. Its involvement in neoplastic development has been studied in gastrointestinal adenocarcinomas. Our paper aims to evaluate the influence of the ghrelin axis in gastrointestinal stromal tumors (GISTs). Materials and Methods: The study design included two groups of patients, 46 with gastric GISTs and 30 with obesity. Archived tissue samples were evaluated for the presence of gastritis and H. pylori. Immunohistochemical expression of ghrelin and its receptor (GHS-R) was assessed. Results: All GISTs showed absent immunohistochemical expression for ghrelin, while GHS-R displayed a particular pattern, with notable differences in intensity (p = 0.0256) and percentage of stained cells (p < 0.00001) in the periphery vs. core of tumors. Positive ghrelin expression was lower in the gastric mucosa of the first group compared to the second group (p < 0.001). Conclusion: The ghrelin axis can influence GISTs carcinogenesis through activation of GHS-R. A previously described direct autocrine/paracrine mechanism is not supported by our findings.
Precision Medicine for Rheumatoid Arthritis: The Right Drug for the Right Patient—Companion Diagnostics
Authors: Meehan Richard Thomas , Amigues Isabelle Anne , Knight Vijaya
Published: July 2021

Despite the growing number of biologic and JAK inhibitor therapeutic agents available to treat various systemic autoimmune illnesses, the lack of a validated companion diagnostic (CDx) to accurately predict drug responsiveness for an individual results in many patients being treated for years with expensive, ineffective, or toxic drugs. This review will focus primarily on rheumatoid arthritis (RA) therapeutics where the need is greatest due to poor patient outcomes if the optimum drug is delayed. We will review current FDA-approved biologic and small molecule drugs and why RA patients switch these medications. We will discuss the sampling of various tissues for potential CDx and review early results from studies investigating drug responsiveness utilizing advanced technologies including; multiplex testing of cytokines and proteins, autoantibody profiling, genomic analysis, proteomics, miRNA analysis, and metabolomics. By using these new technologies for CDx the goal is to improve RA patient outcomes and achieve similar successes like those seen in oncology using precision medicine guided therapeutics.
Segmentation of Melanocytic Lesion Images Using Gamma Correction with Clustering of Keypoint Descriptors
Authors: Okuboyejo Damilola , Olugbara Oludayo O.
Published: July 2021

The early detection of skin cancer, especially through the examination of lesions with malignant characteristics, has been reported to significantly decrease the potential fatalities. Segmentation of the regions that contain the actual lesions is one of the most widely used steps for achieving an automated diagnostic process of skin lesions. However, accurate segmentation of skin lesions has proven to be a challenging task in medical imaging because of the intrinsic factors such as the existence of undesirable artifacts and the complexity surrounding the seamless acquisition of lesion images. In this paper, we have introduced a novel algorithm based on gamma correction with clustering of keypoint descriptors for accurate segmentation of lesion areas in dermoscopy images. The algorithm was tested on dermoscopy images acquired from the publicly available dataset of Pedro Hispano hospital to achieve compelling equidistant sensitivity, specificity, and accuracy scores of 87.29%, 99.54%, and 96.02%, respectively. Moreover, the validation of the algorithm on a subset of heavily noised skin lesion images collected from the public dataset of International Skin Imaging Collaboration has yielded the equidistant sensitivity, specificity, and accuracy scores of 80.59%, 100.00%, and 94.98%, respectively. The performance results are propitious when compared to those obtained with existing modern algorithms using the same standard benchmark datasets and performance evaluation indices.
Clinical Characteristics and Outcome of Patients with Suspected COVID-19 in Emergency Department (RESILIENCY Study II)
Authors: Russo Alessandro , Gentilini Cacciola Elio , Borrazzo Cristian , Filippi Valeria , Bucci Tommaso , Vullo Francesco , Celani Luigi , Binetti Erica , Battistini Luigi , Ceccarelli Giancarlo , Alessandroni Maria , Galardo Gioacchino , Mastroianni Claudio Maria , d’Ettorre Gabriella
Published: July 2021

Objectives: COVID-19 may show no peculiar signs and symptoms that may differentiate it from other infective or non-infective etiologies; thus, early recognition and prompt management are crucial to improve survival. The aim of this study was to describe clinical, laboratory, and radiological characteristics and outcomes of hospitalized COVID-19 patients compared to those with other infective or non-infective etiologies. Methods: We performed a prospective study from March 2020 to February 2021. All patients hospitalized for suspected or confirmed COVID-19 were prospectively recruited. All patients were evaluated according to a predefined protocol for diagnosis of suspected SARS-CoV-2 infection. The primary endpoint was evaluation of clinical, laboratory, and radiological characteristics associated or not with COVID-19 etiology at time of hospitalization in an emergency department. Results: A total of 1036 patients were included in the study: 717 (69%) patients with confirmed COVID-19 and 319 (31%) without COVID-19, hospitalized for other causes. The main causes of hospitalization among non-COVID-19 patients were acute heart failure (44%) and bacterial pneumonia (45.8%). Overall, 30-day mortality was 9% among the COVID-19 group and 35% in the non-COVID-19 group. Multivariate analysis showed variables (fever > 3 days, dry cough, acute dyspnea, lymphocytes < 1000 × 103/µL, and ferritin > 250 ng/mL) independently associated with COVID-19 etiology. A decision tree was elaborated to early detect COVID-19 patients in the emergency department. Finally, Kaplan–Meier curves on 30-day survival in COVID-19 patients during the first wave (March–May 2020, n = 289 patients) and the second wave (October–February 2021, n = 428 patients) showed differences between the two study periods (p = 0.021). Conclusions: Patients with confirmed diagnosis of COVID-19 may show peculiar characteristics at time of hospitalization that could help physicians to distinguish from other infective or non-infective etiologies. Finally, a different 30-day mortality rate was observed during different periods of the pandemic.
Academic Identity and Communities of Practice: Narratives of Social Science Academics Career Decisions in Taiwan
Authors: Ching Gregory Siy
Published: July 2021

Academic identity is an important aspect of organizing an academic career. An academic identity is distinct and unique and can be defined as the core attitudes that determine how individuals approach the concept of work. In the current era of neoliberalism, changes to university governance in Taiwan have transformed working conditions and hiring practices in academia. Inevitably, role conflicts have emerged, and work stress within higher education institutions has increased. The current study summarizes the narratives of nine academics from the social sciences. The study is anchored in the concept that academic identity formation is rooted in the doctoral education stage. Using a qualitative narrative inquiry lens, interactions between different communities of practice during the doctoral education stage are analyzed, along with later career decisions and the role communities of practice play in those decisions. The findings show that doctoral mentors and fellows all contributed to the formation of a core academic identity, while later career decisions were equally affected by neoliberal policies. It is hoped that by recognizing the role of academic identity, administrators may be able to influence how academics adapt amidst the competing pressures within the academe.
Hydraulic Conductivity of Rock Masses Surrounding Water Curtain Boreholes for Underground Oil Storage Caverns
Authors: Zhang Qi-Hua , Liu Qing-Bing , Su Ai-Jun , Li Yu-Jie , Zhang Yi-Hu
Published: July 2021

The assessment of the groundwater flow rate around the cavern periphery is a critical requirement for the design of underground water-sealed oil storage caverns and commonly made through seepage analysis, where a reasonable estimation of the hydraulic conductivity of the host rock is the key issue. However, it is a challenge to accurately determine the hydraulic conductivity of natural rock masses owing to their heterogeneous and anisotropic nature. The underground storage cavern project has a unique favorable condition in that there is a water curtain system that can provide considerable hydraulic test data for inferring hydraulic parameters; however, no well-established method has ever been proposed to exploit these data for characterizing heterogeneity in hydraulic conductivity. This study presents a new approach to evaluate the spatial variation of hydraulic conductivity using water curtain borehole data. This approach treats the peripheral region of each borehole as a homogenous unit with a particular equivalent permeability coefficient that can be back-calculated from the measured injection flow rate of the borehole using a numerically established empirical formula. Besides, the impact of curtain gallery drainage, occurring in the construction stage, on the seepage field was investigated while the effect of the rock fracture configuration on hydraulic conductivity assessment was examined. The proposed method enables robust and accurate mapping of heterogeneity in the hydraulic conductivity of host rocks and provides a new idea of effectively utilizing hydro-geological test data to derive the hydraulic conductivity of rock masses surrounding water-sealed storage caverns.
Risks and Safety of CO2 Transport via Pipeline: A Review of Risk Analysis and Modeling Approaches for Accidental Releases
Authors: Vitali Matteo , Zuliani Cristina , Corvaro Francesco , Marchetti Barbara , Terenzi Alessandro , Tallone Fabrizio
Published: July 2021

Carbon capture and storage is considered an effective mitigation strategy to reduce the most challenging emissions from heavy industries and gas processing. The safe transport of carbon dioxide via pipelines is an important aspect for developing large-scale Carbon Capture and Storage projects. Dispersion modeling for heavy gas such as carbon dioxide is considerably different from natural gas. The set up for modeling simulations is more challenging than conventional natural gas pipeline for several reasons, such as the differences in thermodynamics that must be considered. Moreover, when the carbon dioxide is transported in dense or liquid phase, the rapid phase changing, and possible consequent formation of solids should be considered. Finally, the equation of state required for accurate prediction of parameters is generally different than the ones applicable for natural gas. The main scope of this comprehensive review is to identify the most important parameters, critical events, suitable models, and identification of dispersion modeling issues. An extensive literature review of experiments conducted in the last ten years has been developed, experimental data, integral and simplified model, as well as CFD modeling issues has been identified and reported in the work proposed to highlight the advances and the gaps that could need further research activities.
The Setting of Strength Parameters in Stability Analysis of Open-Pit Slope Using the Random Set Method in the Bełchatów Lignite Mine, Central Poland
Authors: Pilecka Elżbieta , Stanisz Jacek , Kaczmarczyk Robert , Gruchot Andrzej
Published: July 2021

The slopes of open-pit mines are often at risk of failure. To identify this hazard, stability analyses are performed. An important element of these stability analyses is the reliable selection of input parameter values for the calculations. This selection is difficult because the slopes of the open pit are disturbed by mining activities. In such conditions, rheological processes, intensified by weathering, develop in open-pit slopes. This study is aimed at setting the strength parameters for the stability analysis of open-pit slopes with a developed slide process, using the random set method. The study was performed on the example of the open pit of the Bełchatów lignite mine, central Poland. A four-stage methodology, according to the random set method, was proposed. The methodology covered the following: site investigation, sensitivity analyses, shear strength reduction (SSR) analyses using numerical calculations, and probability analyses of the factor of safety (FoS) calculation results. The setting of the input parameters took into account the peak and residual strength parameters for each lithological unit in the physical model of the open-pit slope. Samples for laboratory tests were taken from the cores of nine test boreholes. The sensitivity analysis included all peak and residual strength parameters for each lithological unit in the body. As a result of the sensitivity analysis, specific strength parameters were adopted that would have a great impact upon the results of the calculations. Selected sets of parameter values were then used for the FoS calculations. The resultant FoS values revealed the probable slide planes. The positions of the slide planes were consistent with the interpreted slide surfaces based on the control boreholes and terrain observations. Knowledge of the slide planes positions and the values of the strength parameters enabled the designing of a securing approach for this landslide, and the taking of preventive measures to reduce this risk.
Predicting Water Supply and Evapotranspiration of Street Trees Using Hydro-Pedo-Transfer Functions (HPTFs)
Authors: Wessolek Gerd , Kluge Björn
Published: July 2021

The climate, soil properties, groundwater depth, and surrounding settings in cities vary to a tremendous extent, which all lead to different growing conditions and health for street trees. Because of climate change, the availability of water in cities will undergo changes in the next decades. As urban trees have a very positive influence not only on microclimate but also on biodiversity and life quality in general, they need to be protected. Thus, we need to know how to measure and calculate the availability of water for street trees to optimize their site conditions and water supply. This study presents Hydro-Pedo-Transfer Functions (HTPFs) for predicting water supply and actual evapotranspiration of street trees for varying urban conditions. The HTPFs are easy to use, and the input parameters can either be mapped easily or taken from local climate agencies or soil surveys. The first part of the study focuses on the theoretical background and related assumptions of the HTPFs for predicting water supply, and on obtaining the potential and actual evapotranspiration of urban street trees using easily available data. The second part gives information and exemplifies how this input data can be measured, mapped, or predicted. Calibration of the HTPFs were done using the sap-flow measurements of three Linden trees (Tilia cordata). Exemplarily, the HTPF scenarios for the varying urban site conditions of Berlin are presented. The water supply and actual evapotranspiration of the street trees severely depend on the local climate (summer rainfall and potential evapotranspiration), site conditions (catchment area, soil available water, and degree of sealing), and on the tree characteristics (species, age, and rooting depth). The presented concept and the equations build a good and flexible frame that is easy to program using a spreadsheet tool or an R script. This tool should be tested and validated also for other cities and climate regions.
Survival of Lactobacillus paracasei subsp. paracasei LBC 81 in Fermented Beverage from Chickpeas and Coconut in a Static In Vitro Digestion Model
Authors: Mesquita Maria Carolina , dos Santos Leandro Eliana , Rodrigues de Alencar Ernandes , Botelho Raquel Braz Assunção
Published: July 2021

The objective of this study was to evaluate in a static in vitro digestion model the survival of Lactobacillus paracasei subsp. paracasei LBC 81 in fermented chickpea and coconut beverage. The fermented beverage was stored for 1 and 8 days at 4 °C and then submitted to gastric juice, pancreatic juice, or sequential exposure to gastric and pancreatic juice. The experiment controls were (i) control 1—suspension of cells in 0.85% saline solution; (ii) control 2—cell suspension in chickpea and coconut beverage. The survival of L. paracasei was determined in log CFU/mL and expressed as a survival percentage. The survival of L. paracasei in the fermented beverage after exposure to gastric juice and sequential exposure to gastric and pancreatic juice was 99.47 + 2.05% and 93.21 + 0.43%, respectively. These values were higher than those found for controls 1 and 2. The storage condition of the fermented beverage for 1 or 8 days at 4 °C did not affect the survival after exposure to gastric juice, pancreatic juice, or sequential exposure. The results obtained in this study conclude that the fermented beverage of chickpeas and coconut is an excellent carrier for L. paracasei LBC 81, capable of enhancing survival to gastrointestinal conditions and ensuring a greater number of viable cells reaching the intestinal epithelium.
Electrochemical Determination of Capsaicinoids Content in Soy Sauce and Pot-Roast Meat Products Based on Glassy Carbon Electrode Modified with Β-Cyclodextrin/Carboxylated Multi-Wall Carbon Nanotubes
Authors: Gu Qianhui , Lu Chaoqun , Chen Kangwen , Chen Xingguang , Ma Pengfei , Wang Zhouping , Xu Baocai
Published: July 2021

The rapid quantification of capsaicinoids content is very important for the standardization of pungent taste degree and flavor control of soy sauce and pot-roast meat products. To rapidly quantify the capsaicinoids content in soy sauce and pot-roast meat products, an electrochemical sensor based on β-cyclodextrin/carboxylated multi-wall carbon nanotubes was constructed and the adsorptive stripping voltammetry method was used to enrich samples in this study. The results showed that the excellent performance of the established electrochemical sensor was mostly because β-cyclodextrin caused the relative dispersion of carboxylated multi-wall carbon nanotubes on the glassy carbon electrode surface. Capsaicin and dihydrocapsaicin had similar electrochemical behavior, so the proposed method could determine the total content of capsaicinoids. The linearity of capsaicinoids content was from 0.5 to 100 μmol/L and the detection limit was 0.27 μmol/L. The recovery rates of different capsaicinoids content were between 83.20% and 136.26%, indicating the proposed sensor could realize trace detection of capsaicinoids content in sauce and pot-roast meat products. This work provides a research basis for pungent taste degree standardization and flavor control in the food industry.
Morphology Development and Flow Characteristics during High Moisture Extrusion of a Plant-Based Meat Analogue
Authors: Wittek Patrick , Ellwanger Felix , Karbstein Heike P. , Emin M. Azad
Published: July 2021

Plant-based meat analogues that mimic the characteristic structure and texture of meat are becoming increasingly popular. They can be produced by means of high moisture extrusion (HME), in which protein-rich raw materials are subjected to thermomechanical stresses in the extruder at high water content (>40%) and then forced through a cooling die. The cooling die, or generally the die section, is known to have a large influence on the products’ anisotropic structures, which are determined by the morphology of the underlying multi-phase system. However, the morphology development in the process and its relationship with the flow characteristics are not yet well understood and, therefore, investigated in this work. The results show that the underlying multi-phase system is already present in the screw section of the extruder. The morphology development mainly takes place in the tapered transition zone and the non-cooled zone, while the cooled zone only has a minor influence. The cross-sectional contraction and the cooling generate elongational flows and tensile stresses in the die section, whereas the highest tensile stresses are generated in the transition zone and are assumed to be the main factor for structure formation. Cooling also has an influence on the velocity gradients and, therefore, the shear stresses; the highest shear stresses are generated towards the die exit. The results further show that morphology development in the die section is mainly governed by deformation and orientation, while the breakup of phases appears to play a minor role. The size of the dispersed phase, i.e., size of individual particles, is presumably determined in the screw section and then stays the same over the die length. Overall, this study reveals that morphology development and flow characteristics need to be understood and controlled for a successful product design in HME, which, in turn, could be achieved by a targeted design of the extruders die section.
High Oxygen Packaging of Atlantic Cod Fillets Inhibits Known Spoilage Organisms, but Sensory Quality Is Not Improved Due to the Growth of Carnobacterium/Carnobacteriaceae
Authors: Hansen Anlaug Ådland , Langsrud Solveig , Berget Ingunn , Gaarder Mari Øvrum , Moen Birgitte
Published: July 2021

Improved quality control and prolonged shelf life are important actions in preventing food waste. To get an overview of the bacterial diversity of fillets from live stored mature Atlantic cod, bacterial isolates were identified before and after storage (air and vacuum) and freezing/thawing. Based on the load of dominating bacteria, the effect of different packaging methods and a short freezing/thawing process on prolonged shelf-life was evaluated (total viable counts, bacteriota, sensory attributes, and volatile components). Hand filleted (strict hygiene) cod fillets had a low initial bacterial load dominated by the spoilage organism Photobacterium, whereas industrially produced fillets had higher bacterial loads and diversity (Pseudomonas, Arthrobacter, Psychrobacter, Shewanella). The identified bacteria after storage in vacuum or air were similar to the initially identified bacteria. Bacteriota analysis showed that a short time freezing/thawing process reduced Photobacterium while modified atmosphere packaging (MAP; 60% CO2/40% O2 or 60% CO2/40% N2) inhibited the growth of important spoilage bacteria (Photobacterium,Shewanella, Pseudomonas) and allowed the growth of Carnobacterium/Carnobacteriaceae and Acinetobacter. Despite being dominated by Photobacterium, fresh fillets stored in MAP 60% CO2/40% N2 demonstrated better sensory quality after 13 days of storage than fillets stored in MAP 60% CO2/40% O2 (dominated by Carnobacterium/Carnobacteriaceae). Carnobacterium spp. or other members of Carnobacteriaceae may therefore be potential spoilage organisms in cod when other spoilage bacteria are reduced or inhibited.
HFE Genotype, Ferritin Levels and Transferrin Saturation in Patients with Suspected Hereditary Hemochromatosis
Authors: Sandnes Miriam , Vorland Marta , Ulvik Rune J. , Reikvam Håkon
Published: July 2021

HFE hemochromatosis is characterized by increased iron absorption and iron overload due to variants of the iron-regulating HFE gene. Overt disease is mainly associated with homozygosity for the C282Y variant, although the H63D variant in compound heterozygosity with C282Y (C282Y/H63D) contributes to disease manifestation. In this observational study, we describe the association between biochemical findings, age, gender and HFE genotype in patients referred from general practice to a tertiary care referral center for diagnostic workup based on suspected hemochromatosis due to persistent hyperferritinemia and HFE variants. C282Y and H63D homozygosity were, respectively, the most and least prevalent genotypes and we found a considerable variation in transferrin saturation and ferritin levels independent of HFE genotype, which may indeed represent a diagnostic challenge in general practice. While our results confirm C282Y homozygosity as the major cause of iron accumulation, non-C282Y homozygotes also displayed mild to moderate hyperferritinemia with median ferritin levels at 500–700 µg/L, well above the reference cut-off. Such findings have traditionally been ignored in the clinic, and initiation of iron depletion has largely been restricted to C282Y homozygotes. Nevertheless, superfluous iron can aggravate pathogenesis in combination with other diseases and risk factors, such as inflammation, cancer and hepatopathy, and this possibility should not be neglected by clinicians.