bluuebunny/crossref_metadata_2025 huggingface dataset:
Maternal Vitamin D and Inulin Supplementation in Oxidized Oil Diet Improves Growth Performance and Hepatic Innate Immunity in Offspring Mice
Authors: Xie Guangrong , Zhang Qipeng , Fang Zhengfeng , Che Lianqiang , Lin Yan , Xu Shengyu , Zhuo Yong , Hua Lun , Jiang Xuemei , Li Jian , Sun Mengmeng , Zou Yuanfeng , Huang Chao , Li Lixia , Wu De , Feng Bin
Published: June 2023

Dietary oxidized fat contains harmful materials such as hydrogen peroxide and malondialdehyde (MDA). Excessive oxidized fat intake during pregnancy and lactation not only leads to maternal body injury but also damages offspring health. Our previous study demonstrated that vitamin D (VD) had antioxidative capability in sows. This study was conducted to investigate the effect of maternal VD and inulin supplementation in oxidized oil diet on the growth performance and oxidative stress of their offspring. Sixty 5-month-old C57BL/6N female mice were randomly divided into five groups: Control group (basal diet, n = 12), OF group (oxidized-soybean-oil-replaced diet, n = 12), OFV group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD, n = 12), OFI group (oxidized-soybean-oil-replaced diet + 5% inulin, n = 12) and OFVI group (oxidized-soybean-oil-replaced diet + 7000 IU/kg VD + 5% inulin, n = 12). Mice were fed with the respective diet during pregnancy and lactation. The offspring were then slaughtered on day 21 of age at weaning. Results showed that a maternal oxidized oil diet impaired body weight and liver weight gain of offspring during lactation compared to the control group, while maternal VD, inulin or VD and inulin mixture supplementation reversed this effect. In addition, the activity of T-AOC in the liver of offspring was lower in the OF group than that in the control group, but could be restored by maternal VD and inulin mixture supplementation. Furthermore, the gene expression of both proinflammatory and anti-inflammatory cytokines, such as Il-6, Tnfα and Il-10, in offspring liver were downregulated by a maternal oxidized oil diet compared with the control group, but they were restored by maternal VD or VD and inulin mixture supplementation. The expressions of Vdr and Cyp27a1 were decreased by a maternal oxidized oil diet compared with the control group, while they could be increased by VD or VD and inulin mixture supplementation. Conclusion: maternal oxidized oil diet intake could impair the growth performance by inducing oxidative stress, but this can be relieved by maternal VD and inulin supplementation.
In Vitro Digestion of Peanut Skin Releases Bioactive Compounds and Increases Cancer Cell Toxicity
Authors: Cordeiro-Massironi Karina , Soares-Freitas Rosana Aparecida M. , Sampaio Geni Rodrigues , Pinaffi-Langley Ana Clara da C. , Bridi Raquel , de Camargo Adriano Costa , Torres Elizabeth Aparecida F. S.
Published: June 2023

Peanut skin is a rich source of bioactive compounds which may be able to reduce the risk factors associated with metabolic syndromes. This study aimed to characterize bio-compounds from peanut skin (Arachis hypogaea) and their bioactivity (antioxidant activity, inhibition of lipase, and carbohydrase enzymes) and to evaluate their anti-proliferative properties in colorectal cancer cells (HCT116) upon in vitro digestion. Peanut skin was digested in two sequential phases, and the final content, named phase-1 (P1) and phase-2 (P2) extracts, was evaluated. Several bioactive compounds were positively identified and quantified by liquid chromatography, including quinic acid, released especially after in vitro digestion. The total phenolic content and, regardless of the method, the antioxidant activity of P1 was higher than P2. P1 also showed a lower enzyme inhibitory concentration IC50 than P2, lipase, and α-glucosidase. For cell viability in HCT116 cells, lower concentrations of P1 were found for IC50 compared to P2. In conclusion, bioactive compounds were released mainly during the first phase of the in vitro digestion. The digested samples presented antioxidant activity, enzyme inhibitory activity, and cancer cell cytotoxicity, especially those from the P1 extract. The potential applications of such a by-product in human health are reported.
Quantum Molecular Resonance Inhibits NLRP3 Inflammasome/Nitrosative Stress and Promotes M1 to M2 Macrophage Polarization: Potential Therapeutic Effect in Osteoarthritis Model In Vitro
Authors: Paolucci Teresa , Pino Vanessa , Elsallabi Osama , Gallorini Marialucia , Pozzato Gianantonio , Pozzato Alessandro , Lanuti Paola , Reis Victor Machado , Pesce Mirko , Pantalone Andrea , Buda Roberto , Patruno Antonia
Published: June 2023

This study aimed to investigate the anti-inflammatory effects of Quantum Molecular Resonance (QMR) technology in an in vitro model of osteoarthritis-related inflammation. The study used THP-1-derived macrophages stimulated with lipopolysaccharide and hyaluronic acid fragments to induce the expression of inflammatory cytokines and nitrosative stress. QMR treatment inhibited COX-2 and iNOS protein expression and activity and reduced NF-κB activity. Furthermore, QMR treatment led to a significant reduction in peroxynitrite levels, reactive nitrogen species that can form during inflammatory conditions, and restored tyrosine nitration values to those similar to sham-exposed control cells. We also investigated the effect of QMR treatment on inflammasome activation and macrophage polarization in THP-1-derived macrophages. Results showed that QMR treatment significantly decreased NLRP3 and activated caspase-1 protein expression levels and downregulated IL-18 and IL-1β protein expression and secretion. Finally, our findings indicate that QMR treatment induces a switch in macrophage polarization from the M1 phenotype to the M2 phenotype.
Study on the Partial Surface Discharge Process of Oil-Paper Insulated Transformer Bushing with Defective Condenser Layer
Authors: Yang Fan , Zhang Yuchen , Wu Xingwang , Wu Jie
Published: June 2023

Oil-impregnated paper condenser transformer bushings are an important part of transformer equipment, and partial discharge (PD) occurred when defects exist on the condenser aluminum foil layers. Firstly, to study the PD process of the oil-paper insulated capacitance graded bushing with the defect of broken aluminum foil, a defective oil-paper bushing discharge sample is constructed to study the PD parameters and capacitance, and to discharge carbonization traces at different voltage levels. Then, in order to verify the process of condenser aluminum foil layer discharge and the space charge variation in the oil-paper insulation system of a sample model, the surface flashovers of a needle-plane discharge model based on the bipolar charge transport model and the hydrodynamic model was built. The simulation, by Transport of Diluted Species physics of COMSOL Multiphysics software, points out the discharge process of aluminum foil electrode caused by space charge action and electric field distortion under an electric field at different voltages. The results of simulation and sample bushing experiments showed that the PD process of the defective condenser foil layer is mainly divided into three stages: tip corona discharge, streamer in oil, and surface flashovers. The voltage amplitude is larger the more electrical branches are discharged and the shorter the discharge time is. The findings of the article have important implications for the discharge of the foil layer inside the oil-paper bushing.
Exploring the Key Characteristics and Theoretical Framework for Research on the Metaverse
Authors: Song Chuanxiang , Shin Seong-Yoon , Shin Kwang-Seong
Published: June 2023

This study presents an insightful examination of the conceptual and practical facets of the Metaverse by establishing a novel theoretical framework underpinned by an empirical case study of the Sandbox platform. Anchored in the principles of legality, virtual-reality integration, technological affinity, and community-driven innovation, the paper elucidates the inherent characteristics and potentialities of the Metaverse. Through meticulous research, the paper investigates the antecedents and evolution of the Metaverse, postulating an open, decentralized, and self-regulating ecosystem predicated on user-generated content and engagement. Furthermore, an in-depth case study of the Sandbox elucidates the practical applications, challenges, and opportunities associated with the operationalization of the Metaverse. The study showcases how avant-garde technologies such as blockchain, virtual reality, and artificial intelligence are instrumental in fostering immersive experiences, safeguarding virtual asset ownership, and facilitating tailored services. Moreover, the paper accentuates the indispensable role of community engagement and continuous innovation in cultivating a flourishing Metaverse environment. The analysis exposes that the burgeoning development of the Metaverse is intrinsically linked to the amalgamation of the virtual and the tangible, extending the frontiers of the digital economy. While shedding light on the virtues of the Metaverse, the study recognizes its nascent state and encourages further scholarly inquiry to comprehend and navigate its complexities. This research contributes significantly to the academic and practical understanding of the Metaverse, serving as a cornerstone for future investigations and technological advancements in this paradigm-shifting domain.
Double-Skin Façades for Building Retrofitting and Climate Change: A Case Study in Central Italy
Authors: Lops Camilla , Di Loreto Samantha , Pierantozzi Mariano , Montelpare Sergio
Published: June 2023

In recent years, the need to make the built environment more resilient and adaptable to climate change has become essential. In Europe, this aspect concerns most existing buildings with several deficiencies from the energy efficiency point of view, considering they were designed before the introduction of modern codes. Among the various strategies for building energy retrofitting, Double-Skin Façades (DSFs) have gained attention due to their potential to improve the building performance and inhabitants’ comfort. This research aims to evaluate the use of adequately designed DSFs for the energy restoration of buildings. In detail, various DSF configurations are applied to a residential building located in Central Italy and investigated under present and future climate conditions, estimated through regional climate models. The installation of multi-layered façades, particularly the Multi-Storey typology, greatly reduces energy consumption and increases the expected comfort rates. When the selected configuration was considered, the results underline a decrease in the annual building energy requirement of about 37–56% up to 42–59%, respectively, for 2030 and 2070. Moreover, using multi-layer façades can increase indoor minimum operative temperatures up to 3.8% during the coldest months and reduce the maximum summer ones by 1.9–3.8%, raising comfort levels.
Ship Structures: Design Loads and Reliability Assessment
Authors: Kim Jeong-Hyeon , Kim Seul-Kee , Kim Jeong-Dae , Lee Jae-Myung , Kim Jeong-Hwan
Published: June 2023

In order to operate, ships and offshore structures heavily rely on bunker oil and marine diesel oil [...]
EC-GAN: Emotion-Controllable GAN for Face Image Completion
Authors: Chen Yueqiao , Yang Wenxia , Fang Xi , Han Huan
Published: June 2023

Image completion methods based on deep learning, such as Variational Autoencoders (VAEs) and Generative Adversarial Networks (GANs), have succeeded in producing semantically plausible results. However, existing facial image completion methods can either produce only one result or, although they can provide multiple results, cannot attribute particular emotions to the results. We propose EC-GAN, a novel facial Emotion-Controllable GAN-based image completion model that can infer and customize generative facial emotions. We propose an emotion inference module that infers the emotions of faces based on the unmasked regions of the faces. The emotion inference module is trained in a supervised manner and enforces the encoder to disentangle the emotion semantics from the native latent space. We also developed an emotion control module to modify the latent codes of emotions, moving the latent codes of the initial emotion toward the desired one while maintaining the remaining facial features. Extensive experiments were conducted on two facial datasets, CelebA-HQ and CFEED. Quantitative and qualitative results indicate that EC-GAN produces images with diverse desired expressions even when the main features of the faces are masked. On the other hand, EC-GAN promotes semantic inference capability with irregularly masked holes, resulting in more natural facial expressions.
Designing a C-ITS Communication Infrastructure for Traffic Signal Priority of Public Transport
Authors: Otto Thomas , Partzsch Ina , Holfeld Jörg , Klöppel-Gersdorf Michael , Ivanitzki Victor
Published: June 2023

Looking ahead: transforming conventional public transport prioritization into C-ITS G5 services. The city of Frankfurt aims to digitize its public transport prioritization system in order to fulfill the requirements of future public transport communication standards and, moreover, to build on this very infrastructure for the development of imminent C-ITS services. Therefore, the communication systems of the mobility and transport provider VGF (Verkehrsgesellschaft Frankfurt am Main mbH) are being revised fundamentally by implementing new technologies for Car2X C-ITS G5 communication. The hardware components of the C-ITS system are strategically positioned with the help of a newly developed planning tool that identifies and determines the range of communication. For highly significant sites and locations of the hardware components, the calculated data are validated by utilizing measurements within a mobile setup. The operational stability and the development of previously unused potential are then carried out via the combination of the C-ITS services TSP (Traffic Signal Priority) and GLOSA (Green Light Optimized Speed Advisory). The overlay of the C-ITS services results in a high level of operational stability. As a result, potentials can be adequately employed through the sensible shifting of waiting times to the stops and a smooth flow of traffic through information on optimal speed and remaining times of the traffic light potentials. This paper presents a new methodology with which it is now possible to plan and evaluate C-ITS with regard to service distribution and radio propagation.
Modular Bus Unit Scheduling for an Autonomous Transit System under Range and Charging Constraints
Authors: Gao Hong , Liu Kai , Wang Jiangbo , Guo Fangce
Published: June 2023

Recent advances in vehicle technology offer new opportunities for an electric, automated, modular bus (MB) unit with an adjustable capacity to be applied to transit systems, promising to tackle the resource allocation challenges of traditional buses in coping with uneven travel demand. Drawing on the concept of modular vehicles, this paper introduces a novel scheduling system in which MB units can be combined/separated from fulfilling imbalanced trip demands through capacity adjustments. We develop an optimization model for determining the optimal formation and trip sequence of MB units. In particular, given that the vehicles are electrically powered, battery range limits and charging plans are considered in the system scheduling process. A column-generation-based heuristic algorithm is designed to efficiently solve this model, with constraints related to travel demand and charging station capacity incorporated into the master problem and the trip sequence for modular units with limited energy solved by the subproblem. Taking real data from transit operations for numerical examples, the proposed model performs well in terms of both algorithmic performance and practical applications. The generated optimal MB dispatching scheme can significantly reduce the operating cost from $1534.31 to $1144.26, a decrease of approximately 25% compared to conventional electric buses. The sensitivity analysis on the MB dispatch cost and battery capacity provides some insights for both the scenario configuration and the battery selection for MB system implementation.
Study on the Surface Morphology of Thermochromic Rf-Sputtered VO2 Films Using Temperature-Dependent Atomic Force Microscopy
Authors: Gagaoudakis Emmanouil , Verveniotis Elisseos , Okawa Yuji , Michail Giannis , Aperathitis Elias , Mantsiou Eleni , Kiriakidis George , Binas Vassilios
Published: June 2023

Vanadium dioxide (VO2) is a well-known phase-changing material that goes from a semiconducting state to a metallic one at a critical temperature of 68 °C, which is the closest to room temperature (25 °C). The electrical transition is also accompanied by structural and optical changes. The optical transition upon heating-also known as thermochromism-makes VO2 a possible coating for “intelligent” windows. In this work, the relationship between the thermochromic performance of VO2 films and the surface morphology was investigated using Temperature-dependent Atomic Force Microscopy (T-AFM) in conjunction with the X-ray Diffraction technique and Scanning Electron Microscopy. In particular, VO2 films were deposited using the rf sputtering technique on Silicon and glass substrates at a substrate temperature of 300 °C, which is one of the lowest for this technique to grow the thermochromic monoclinic phase of VO2. It was found that upon heating (25–100 °C), there was a decrease in RMS roughness for all films independent from the substrate; the value of RMS roughness, however, varied depending on the substrate. Finally, the thermochromic parameters of the VO2 films were correlated with the surface morphology and appeared to be dependent on the kind of substrate used.
Development and Evaluation of Topical Formulation Containing Agrimonia pilosa Extract
Authors: Lee Jin Seok , Nam Yu Ran , Park Su Jin , Lee Ji Min , Kim Hyun Jong , Kim Woo Kyung
Published: June 2023

Natural products are promising drug candidates with various pharmacological effects. However, they can be difficult to use due to poor bioavailability or low stability. In this experiment, while developing topical formulations containing 0.1% Agrimonia pilosa extract, a simple and rapid method of analyzing flavonoid glucuronides, which are representative ingredients, was developed and validated, and the physicochemical properties and stability of flavonoid glucuronides were observed. As a result, an optimized cream formulation was developed. The oil phase comprised tween 60, liquid paraffin, propylene glycol, cetanol, stearyl alcohol, span 60, benzyl alcohol, and A. pilosa extracts, and the water phase comprised water and citric acid hydrate. Then, physicochemical and microbial stability tests of the formulation were conducted under long-term (12 months) and accelerated conditions (6 months). It was thus confirmed that both physicochemical and biological properties were stable during the test period. Consequently, an optimized formulation for the extract was developed and stability was confirmed.
Robust Medical Image Watermarking Scheme Using PSO, LWT, and Hessenberg Decomposition
Authors: Kumar Lalan , Singh Kamred Udham , Kumar Indrajeet , Kumar Ankit , Singh Teekam
Published: June 2023

Digital imaging is a technology that is extensively employed in diverse diagnostic examinations such as magnetic resonance imaging (MRI), computed tomography (CT), and ultrasound imaging, among other modalities. Transferring a patient’s diagnostic images and medical data to a specialist physician in a distinct geographical location is conducted to facilitate an accurate diagnosis. The safeguarding of patient data privacy and confidentiality is ensured through the utilisation of smart hospital applications for medical data security. The current research presents the effective utilisation of lifting wavelet transform (LWT) and Hessenberg-based particle swarm optimization in order to generate resilient and safeguarded watermarks on ultrasound images. The empirical evidence suggests that our innovative approach outperforms our prior methodology, established through extensive testing. The watermark’s imperceptibility and accuracy are exemplified by its capacity to sustain a superior structural similarity index measure (SSIM) and peak signal-to-noise ratio (PSNR), even amidst diverse image processing assaults.
Special Issue on New Challenges in Serious Game Design
Authors: Xinogalos Stelios , Satratzemi Maya
Published: June 2023

Serious games are games that have a “serious” purpose besides entertainment [...]
Design of a Functionally Graded Material Phonon Crystal Plate and Its Application in a Bridge
Authors: Li Shuqin , Song Jing , Ren Jingshun
Published: June 2023

In order to alleviate the structural vibrations induced by traffic loads, in this paper, a phonon crystal plate with functionally graded materials is designed based on local resonance theory. The vibration damping performance of the phonon crystal plate is studied via finite element numerical simulation and the band gap is verified via vibration transmission response analysis. Finally, the engineering application mode is simulated to make it have practical engineering application value. The results show that the phonon crystal plate has two complete bandgaps within 0~150 Hz, the initial bandgap frequency is 0.00 Hz, the cut-off frequency is 128.32 Hz, and the internal ratio of 0~100 Hz is 94.13%, which can effectively reduce the structural vibration caused by traffic loads. Finally, stress analysis of the phonon crystal plate is carried out. The results show that phonon crystals of functionally graded materials can reduce stress concentration through adjusting the band gap. The phonon crystal plate designed in this paper can effectively suppress the structural vibration caused by traffic loads, provides a new method for the vibration reduction of traffic infrastructure, and can be applied to the vibration reduction of bridges and their auxiliary facilities.
Fragments for the History of an Architecture: A Villa between Humanism and the Renaissance
Authors: Mileto Camilla , Vegas Fernando
Published: June 2023

This article presents a detailed study of the stately palace of the Villa Giusti-Puttini, a building that, over the centuries, has undergone repeated transformations since its construction in the first half of the 15th century. For the study of this palace, owned between the 15th and 17th centuries by one of the most important families in the city of Verona (Italy), the authors have followed a methodology covering indirect sources (documentary and bibliographical) as well as direct ones (the building and constructive techniques, architectural and decorative elements, murals, etc.). This study expands the information available on the building as well as expanding knowledge on the history of architecture of the Veneto villa as a defining architectural phenomenon in 16th- and 17th-century architecture whose extensive influence was still felt in the 19th century. The history compiled through this research also contributes to a renewed interpretation of the phenomenon, which is viewed as a process for the transformation and adaptation of a pre-existing building to fit the needs of any given period. This methodology, which could potentially facilitate the interpretation of similar buildings, and its combination of documentary, material, constructive, decorative, and cultural elements could constitute an example for the historical and architectural reading of buildings and are not merely limited to Renaissance buildings.
“Experiencing Trauma”: Aesthetical, Sensational and Narratological Issues of Traumatic Representations in Slasher Horror Cinema
Authors: Groh Florentin
Published: June 2023

In the field of horror film studies, the question of trauma is generally related to the spectator’s experience. The trauma of images occurs in the context of socio-cultural actualization. The degree of violence involved in the images, either graphic or symbolic, implies an experience that marks the viewer. Trauma, in this way, acts as a sensitive degree of perception, the image being an event. We start from this theoretical point but decide to take as our object of study only films where the horrific experience is based on a figurative representation of trauma. Therefore, we want to detach ourselves from a symbolic reading of the horrific image, leaving aside the psychological implications of the image’s effect. We decide to adopt a phenomenological and enactive reading of the image in order to include our spectatorial sensations in the narrative and aesthetic analysis of the representations issues of trauma as a horrific experience. Thus, in our corpus, trauma does not intervene in the cognitive formation of the spectator but is built into the experience of the filmic corpse according to a visual and narrative continuity specific to the films. We designate two types of traumatic events that occur in the corpus films: Halloween II; Friday the 13th: A New Beginning. We try to understand the emergence of the traumatic feeling within the spectator and demonstrate that the trauma experienced by the viewer arises from the horrific experience specific to the aesthetic and narrative aims of the films, mirroring the symptoms and the wounds of the characters.
Projection and Analysis of Floods in the Upper Heihe River Basin under Climate Change
Authors: Ye Yingtao , Li Zhanling , Li Xintong , Li Zhanjie
Published: June 2023

The projection of future hydrological processes can provide insights into the risks associated with potential hydrological events in a changing environment and help develop strategies to cope with and prevent them. The Heihe River basin in Northwest China is crucial for providing water resources to water-scarce regions. Thus, understanding the future runoff trends in the context of climate change can optimize water allocation, alleviate water shortages, and mitigate flood risks in the region. In this study, we use meteorological data from 10 general circulation models under two future scenarios to drive the Soil and Water Assessment Tool (SWAT) model and project hydrological processes in the upper Heihe River basin from 2026 to 2100. After examining the future changes in total runoff in the basin, we assess the magnitude, frequency, and timing of daily flood events in the future. The results of the multi-model ensemble averaging (MMEA) method show that the change in the multi-year average annual runoff is −4.5% (2026–2050), −1.8% (2051–2075), and +2.0% (2076–2100) under the SSP245 scenario and −1.0% (2026–2050), +0.4% (2051–2075), and +0.2% (2076–2100) under the SSP585 scenario compared to the historical period. The analysis of flood magnitudes indicates that the basin will experience higher-magnitude floods in the future, with the largest increase rates of 61.9% and 66.4% for the 1-day maximum flows under the SSP245 and SSP585 scenarios, respectively. The flood return period is projected to be shorter in the future, and the 1-day maximum flows of a 100-year flood are expected to increase by 44.7% and 63.7% under the SSP245 and SSP585 scenarios, respectively. Furthermore, a significant shift in the flood timing is expected, with the highest frequency moving from July to August, representing a one-month lag compared to the historical period. Our findings suggest that the hydrological characteristics of the upper Heihe River basin may be significantly altered in the future due to the effects of climate change, resulting in floods with higher magnitudes and frequencies and different timings. Therefore, it is imperative to consider these changes carefully when developing risk prevention measures.
A Learning—Based Particle Swarm Optimizer for Solving Mathematical Combinatorial Problems
Authors: Olivares Rodrigo , Soto Ricardo , Crawford Broderick , Ríos Víctor , Olivares Pablo , Ravelo Camilo , Medina Sebastian , Nauduan Diego
Published: June 2023

This paper presents a set of adaptive parameter control methods through reinforcement learning for the particle swarm algorithm. The aim is to adjust the algorithm’s parameters during the run, to provide the metaheuristics with the ability to learn and adapt dynamically to the problem and its context. The proposal integrates Q–Learning into the optimization algorithm for parameter control. The applied strategies include a shared Q–table, separate tables per parameter, and flexible state representation. The study was evaluated through various instances of the multidimensional knapsack problem belonging to the NP-hard class. It can be formulated as a mathematical combinatorial problem involving a set of items with multiple attributes or dimensions, aiming to maximize the total value or utility while respecting constraints on the total capacity or available resources. Experimental and statistical tests were carried out to compare the results obtained by each of these hybridizations, concluding that they can significantly improve the quality of the solutions found compared to the native version of the algorithm.
A Generalized Norm on Reproducing Kernel Hilbert Spaces and Its Applications
Authors: Altwaijry Najla , Feki Kais , Minculete Nicuşor
Published: June 2023

The aim of this article was to provide improved estimates for the (α,β)-norm of a bounded linear operator. In particular, our results enabled the determination of new upper bounds involving both the Berezin number and the Berezin norm of bounded linear operators that act on reproducing kernel Hilbert spaces. Through our analysis, we hoped to enhance the understanding of the properties and behavior of such operators and contribute to the development of new mathematical tools for their characterization and application.
Existence, Approximation and Stability of Fixed Point for Cirić Contraction in Convex b-Metric Spaces
Authors: Gazal  , Rathee Savita , Mahima  , Kadyan Anshuka , Minakshi  , Kumar Anil
Published: June 2023

We establish a new fixed point theorem in the setting of convex b-metric spaces that ensures the existence of fixed point for Cirić contraction with the assumption k<1s2. Also, the fixed point is approximated by Krasnoselskij iterative procedure. Moreover, we discuss the stability of fixed point for the aforesaid contraction. As a consequence, we develop a common fixed point and coincidence point result. Finally, we provide a number of examples to illustrate the findings presented here and incorporate these findings to solve an initial value problem.
High Flashpoint and Eco-Friendly Electrolyte Solvent for Lithium-Ion Batteries
Authors: Ströbel Marco , Kiefer Larissa , Pross-Brakhage Julia , Hemmerling Jessica , Finster Philipp , Ziebert Carlos , Birke Kai Peter
Published: June 2023

Since Sony launched the commercial lithium-ion cell in 1991, the composition of the liquid electrolytes has changed only slightly. The electrolyte consists of highly flammable solvents and thus poses a safety risk. Solid-state ion conductors, classified as non-combustible and safe, are being researched worldwide. However, they still have a long way to go before being available for commercial cells. As an alternative, this study presents glyceryl tributyrate (GTB) as a flame retardant and eco-friendly solvent for liquid electrolytes for lithium-ion cells. The remarkably high flashpoint (TFP=174°C) and the boiling point (TBP=287°C) of GTB are approximately 150 K higher than that of conventional linear carbonate components, such as ethyl methyl carbonate (EMC) or diethyl carbonate (DEC). The melting point (TMP=−75°C) is more than 100 K lower than that of ethylene carbonate (EC). A life cycle test of graphite/NCM with 1 M LiTFSI dissolved in GTB:EC (85:15 wt) achieved a Coulombic efficiency of above 99.6% and the remaining capacity resulted in 97% after 50 cycles (C/4) of testing. The flashpoint of the created electrolyte is TFP=172°C and, therefore, more than 130 K higher than that of state-of-the-art liquid electrolytes. Furthermore, no thermal runaway was observed during thermal abuse tests. Compared to the reference electrolyte LP40, the conductivity of the GTB-based is reduced, but the electrochemical stability is highly improved. GTB-based electrolytes are considered an interesting alternative for improving the thermal stability and safety of lithium-ion cells, especially in low power-density applications.
The Kinematics of Fixed-Seat Rowing: A Structured Synthesis
Authors: Agius Tonio P. , Cerasola Dario , Gauci Michael , Sciriha Anabel , Sillato Darren , Formosa Cynthia , Gatt Alfred , Xerri de Caro John , Needham Robert , Chockalingam Nachiappan , Grima Joseph N.
Published: June 2023

Olympic-style sliding-seat rowing is a sport that has been extensively researched, with studies investigating aspects related to the physiology, biomechanics, kinematics, and the performance of rowers. In contrast, studies on the more classic form of fixed-seat rowing are sparse. The aim of this study is to address this lacuna by analysing for the first time the specific kinematics of fixed-seat rowing as practised by able-bodied athletes, thus (i) documenting how this technique is performed in a manner that is replicable by others and (ii) showing how this technique compares and contrasts with the more standard sliding-seat technique. Fixed-seat rowing was replicated in a biomechanics laboratory where experienced fixed-seat rowers, marked with reflective markers following the modified Helen–Hayes model, were asked to row in a manner that mimics rowing on a fixed-seat boat. The findings from this study, complimented with data gathered through the observation of athletes rowing on water, were compared to sliding-seat ergometer rowing and other control experiments. The results show that, in fixed-seat rowing, there is more forward and backward thoracic movement than in sliding-seat rowing (75–77° vs. 44–52°, p < 0.0005). Tilting of the upper body stems was noted to result from rotations around the pelvis, as in sliding-seat rowing, rather than from spinal movements. The results also confirmed knee flexion in fixed-seat rowing with a range of motion of 30–35°. This is less pronounced than in standard-seat rowing, but not insignificant. These findings provide a biomechanical explanation as to why fixed-seat rowers do not have an increased risk of back injuries when compared with their sliding-seat counterparts. They also provide athletes, coaches, and related personnel with precise and detailed information of how fixed-seat rowing is performed so that they may formulate better and more specific evidence-based training programs to meliorate technique and performance.
MicroRNA (miR)-124: A Promising Therapeutic Gateway for Oncology
Authors: Gourishetti Karthik , Balaji Easwaran Vignesh , Mostakim Youssef , Ranganath Pai K. Sreedhara , Bhere Deepak
Published: June 2023

MicroRNA (miR) are a class of small non-coding RNA that are involved in post-transcriptional gene regulation. Altered expression of miR has been associated with several pathological conditions. MicroRNA-124 (miR-124) is an abundantly expressed miR in the brain as well as the thymus, lymph nodes, bone marrow, and peripheral blood mono-nuclear cells. It plays a key role in the regulation of the host immune system. Emerging studies show that dysregulated expression of miR-124 is a hallmark in several cancer types and it has been attributed to the progression of these malignancies. In this review, we present a comprehensive summary of the role of miR-124 as a promising therapeutic gateway in oncology.
MiR-23a Regulates Skin Langerhans Cell Phagocytosis and Inflammation-Induced Langerhans Cell Repopulation
Authors: Wang Jie , Parajuli Nirmal , Wang Qiyan , Khalasawi Namir , Peng Hongmei , Zhang Jun , Yin Congcong , Mi Qing-Sheng , Zhou Li
Published: June 2023

Langerhans cells (LCs) are skin-resident macrophage that act similarly to dendritic cells for controlling adaptive immunity and immune tolerance in the skin, and they are key players in the development of numerous skin diseases. While TGF-β and related downstream signaling pathways are known to control numerous aspects of LC biology, little is known about the epigenetic signals that coordinate cell signaling during LC ontogeny, maintenance, and function. Our previous studies in a total miRNA deletion mouse model showed that miRNAs are critically involved in embryonic LC development and postnatal LC homeostasis; however, the specific miRNA(s) that regulate LCs remain unknown. miR-23a is the first member of the miR-23a-27a-24-2 cluster, a direct downstream target of PU.1 and TGF-b, which regulate the determination of myeloid versus lymphoid fates. Therefore, we used a myeloid-specific miR-23a deletion mouse model to explore whether and how miR-23a affects LC ontogeny and function in the skin. We observed the indispensable role of miR-23a in LC antigen uptake and inflammation-induced LC epidermal repopulation; however, embryonic LC development and postnatal homeostasis were not affected by cells lacking miR23a. Our results suggest that miR-23a controls LC phagocytosis by targeting molecules that regulate efferocytosis and endocytosis, whereas miR-23a promotes homeostasis in bone marrow-derived LCs that repopulate the skin after inflammatory insult by targeting Fas and Bcl-2 family proapoptotic molecules. Collectively, the context-dependent regulatory role of miR-23a in LCs represents an extra-epigenetic layer that incorporates TGF-b- and PU.1-mediated regulation during steady-state and inflammation-induced repopulation.
Inbreeding-Driven Innate Behavioral Changes in Drosophila melanogaster
Authors: Amanullah Anusha , Arzoo Shabana , Aslam Ayesha , Qureshi Iffat Waqar , Hussain Mushtaq
Published: June 2023

Drosophila melanogaster has long been used to demonstrate the effect of inbreeding, particularly in relation to reproductive fitness and stress tolerance. In comparison, less attention has been given to exploring the influence of inbreeding on the innate behavior of D. melanogaster. In this study, multiple replicates of six different types of crosses were set in pair conformation of the laboratory-maintained wild-type D. melanogaster. This resulted in progeny with six different levels of inbreeding coefficients. Larvae and adult flies of varied inbreeding coefficients were subjected to different behavioral assays. In addition to the expected inbreeding depression in the-egg to-adult viability, noticeable aberrations were observed in the crawling and phototaxis behaviors of larvae. Negative geotactic behavior as well as positive phototactic behavior of the flies were also found to be adversely affected with increasing levels of inbreeding. Interestingly, positively phototactic inbred flies demonstrated improved learning compared to outbred flies, potentially the consequence of purging. Flies with higher levels of inbreeding exhibited a delay in the manifestation of aggression and courtship. In summary, our findings demonstrate that inbreeding influences the innate behaviors in D. melanogaster, which in turn may affect the overall biological fitness of the flies.
Considering the Value of 3D Cultures for Enhancing the Understanding of Adhesion, Proliferation, and Osteogenesis on Titanium Dental Implants
Authors: Ferro Federico , Azzolin Federico , Spelat Renza , Bevilacqua Lorenzo , Maglione Michele
Published: June 2023

Background: Individuals with pathologic conditions and restorative deficiencies might benefit from a combinatorial approach encompassing stem cells and dental implants; however, due to the various surface textures and coatings, the influence of titanium dental implants on cells exhibits extensive, wide variations. Three-dimensional (3D) cultures of stem cells on whole dental implants are superior in testing implant properties and were used to examine their capabilities thoroughly. Materials and methods: The surface micro-topography of five titanium dental implants manufactured by sandblasting with titanium, aluminum, corundum, or laser sintered and laser machined was compared in this study. After characterization, including particle size distribution and roughness, the adhesion, proliferation, and viability of adipose-derived stem cells (ADSCs) cultured on the whole-body implants were tested at three time points (one to seven days). Finally, the capacity of the implant to induce ADSCs’ spontaneous osteoblastic differentiation was examined at the same time points, assessing the gene expression of collagen type 1 (coll-I), osteonectin (osn), alkaline phosphatase (alp), and osteocalcin (osc). Results: Laser-treated (Laser Mach and Laser Sint) implants exhibited the highest adhesion degree; however, limited proliferation was observed, except for Laser Sint implants, while viability differences were seen throughout the three time points, except for Ti Blast implants. Sandblasted surfaces (Al Blast, Cor Blast, and Ti Blast) outpaced the laser-treated ones, inducing higher amounts of coll-I, osn, and alp, but not osc. Among the sandblasted surfaces, Ti Blast showed moderate roughness and the highest superficial texture density, favoring the most significant spontaneous differentiation relative to all the other implant surfaces. Conclusions: The results indicate that 3D cultures of stem cells on whole-body titanium dental implants is a practical and physiologically appropriate way to test the biological characteristics of the implants, revealing peculiar differences in ADSCs’ adhesion, proliferation, and activity toward osteogenic commitment in the absence of specific osteoinductive cues. In addition, the 3D method would allow researchers to test various implant surfaces more thoroughly. Integrating with preconditioned stem cells would inspire a more substantial combinatorial approach to promote a quicker recovery for patients with restorative impairments.
ER Stress, the Unfolded Protein Response and Osteoclastogenesis: A Review
Authors: Huang Wangli , Gong Yining , Yan Liang
Published: June 2023

Endoplasmic reticulum (ER) stress and its adaptive mechanism, the unfolded protein response (UPR), are triggered by the accumulation of unfolded and misfolded proteins. During osteoclastogenesis, a large number of active proteins are synthesized. When an imbalance in the protein folding process occurs, it causes osteoclasts to trigger the UPR. This close association has led to the role of the UPR in osteoclastogenesis being increasingly explored. In recent years, several studies have reported the role of ER stress and UPR in osteoclastogenesis and bone resorption. Here, we reviewed the relevant literature and discussed the UPR signaling cascade response, osteoclastogenesis-related signaling pathways, and the role of UPR in osteoclastogenesis and bone resorption in detail. It was found that the UPR signal (PERK, CHOP, and IRE1-XBP1) promoted the expression of the receptor activator of the nuclear factor-kappa B ligand (RANKL) in osteoblasts and indirectly enhanced osteoclastogenesis. IRE1 promoted osteoclastogenesis via promoting NF-κB, MAPK signaling, or the release of pro-inflammatory factors (IL-6, IL-1β, and TNFα). CREBH promoted osteoclast differentiation by promoting NFATc1 expression. The PERK signaling pathway also promoted osteoclastogenesis through NF-κB and MAPK signaling pathways, autophagy, and RANKL secretion from osteoblasts. However, salubrinal (an inhibitor of eIF2α dephosphorylation that upregulated p-eIF2α expression) directly inhibited osteoclastogenesis by suppressing NFATc1 expression and indirectly promoted osteoclastogenesis by promoting RANKL secretion from osteoblasts. Therefore, the specific effects and mechanisms of p-PERK and its downstream signaling on osteoclastogenesis still need further experiments to confirm. In addition, the exact role of ATF6 and BiP in osteoclastogenesis also required further exploration. In conclusion, our detailed and systematic review provides some references for the next step to fully elucidate the relationship between UPR and osteoclastogenesis, intending to provide new insights for the treatment of diseases caused by osteoclast over-differentiation, such as osteoporosis.
The Evaluation of SWEEPS Plus Antimicrobial Photodynamic Therapy with Indocyanine Green in Eliminating Enterococcus faecalis Biofilm from Infected Root Canals: An In Vitro Study
Authors: Rostami Golriz , Afrasiabi Shima , Benedicenti Stefano , Signore Antonio , Chiniforush Nasim
Published: June 2023

Objectives: This study aimed to assess the efficacy of shockwave-enhanced emission photoacoustic streaming (SWEEPS) plus antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) for the elimination of Enterococcus faecalis biofilm from infected root canals. Materials and Methods: thirty sound human single-canal teeth were chosen and standardized to have 12 mm of root length. The root canals were shaped and prepared by means of ProTaper rotary files. After sterilization of the teeth, the canals were inoculated with E. faecalis for 2 weeks. The teeth were then randomly divided into six groups (n = five) of control, ICG, ICG + 808 nm diode laser, ICG + SWEEPS, ICG + 808 nm diode laser + SWEEPS, and 5.25% sodium hypochlorite (NaOCl). Following treatment, the number of colony-forming units (CFUs)/mL were calculated for each group. Statistical analysis was carried out using one-way ANOVA. For multiple comparisons, Tukey’s test was used as the post hoc test. Results: NaOCl alone showed the highest efficacy (p < 0.001). The ICG + 808 nm diode laser + SWEEPS group displayed significantly lower amounts of bacteria than either the ICG + 808 nm diode laser or SWEEPS (p < 0.001). There was a statistically significant difference detected between the ICG + 808 nm diode laser and ICG + SWEEPS (p = 0.035). Conclusions: SWEEPS can effectively increase the photosensitizer distribution in the root canal space, and its application along with irrigants can bring about promising results.
Biomarkers for Hepatocellular Carcinoma: From Origin to Clinical Diagnosis
Authors: Omar Mona A. , Omran Mohamed M. , Farid Khaled , Tabll Ashraf A. , Shahein Yasser E. , Emran Tarek M. , Petrovic Ana , Lucic Nikola R. , Smolic Robert , Kovac Tanja , Smolic Martina
Published: June 2023

The incidence of hepatocellular carcinoma (HCC) and HCC-related deaths has increased over the last few decades. There are several risk factors of HCC such as viral hepatitis (B, C), cirrhosis, tobacco and alcohol use, aflatoxin-contaminated food, pesticides, diabetes, obesity, nonalcoholic fatty liver disease (NAFLD), and metabolic and genetic diseases. Diagnosis of HCC is based on different methods such as imaging ultrasonography (US), multiphasic enhanced computed tomography (CT), magnetic resonance imaging (MRI), and several diagnostic biomarkers. In this review, we examine the epidemiology of HCC worldwide and in Egypt as well as risk factors associated with the development of HCC and, finally, provide the updated diagnostic biomarkers for the diagnosis of HCC, particularly in the early stages of HCC. Several biomarkers are considered to diagnose HCC, including downregulated or upregulated protein markers secreted during HCC development, circulating nucleic acids or cells, metabolites, and the promising, recently identified biomarkers based on quantitative proteomics through the isobaric tags for relative and absolute quantitation (iTRAQ). In addition, a diagnostic model used to improve the sensitivity of combined biomarkers for the diagnosis of early HCC is discussed.