bluuebunny/crossref_metadata_2025 huggingface dataset:
Selected Papers from the 2017 International Conference on Micro/Nanomachines
Authors: Mou Fangzhi , Guan Jianguo
Published: June 2018

Thanks to their capabilities of converting various energy into motions, micro/nanomachines are believed to bring about revolutionary changes in many fields[...]
Irinotecan and Δ9-Tetrahydrocannabinol Interactions in Rat Liver: A Preliminary Evaluation Using Biochemical and Genotoxicity Markers
Authors: Lucić Vrdoljak Ana , Fuchs Nino , Mikolić Anja , Žunec Suzana , Brčić Karačonji Irena , Jurič Andreja , Prester Ljerka , Micek Vedran , Neuberg Marijana , Čanović Samir , Mršić Gordan , Kopjar Nevenka
Published: June 2018

There is growing interest regarding the use of herbal preparations based on Cannabis sativa for medicinal purposes, despite the poorly understood interactions of their main constituent Δ9-tetrahydrocannabinol (THC) with conventional drugs, especially cytostatics. The objective of this pilot study was to prove whether the concomitant intake of THC impaired liver function in male Wistar rats treated with the anticancer drug irinotecan (IRI), and evaluate the toxic effects associated with this exposure. IRI was administered once intraperitoneally (at 100 mg/kg of the body weight (b.w.)), while THC was administered per os repeatedly for 1, 3, and 7 days (at 7 mg/kg b.w.). Functional liver impairments were studied using biochemical markers of liver function (aspartate aminotransferase—AST, alanine aminotransferase—ALP, alkaline phosphatase—AP, and bilirubin) in rats given a combined treatment, single IRI, single THC, and control groups. Using common oxidative stress biomarkers, along with measurement of primary DNA damage in hepatocytes, the degree of impairments caused at the cellular level was also evaluated. THC caused a time-dependent enhancement of acute toxicity in IRI-treated rats, which was confirmed by body and liver weight reduction. Although single THC affected ALP and AP levels more than single IRI, the levels of liver function markers measured after the administration of a combined treatment mostly did not significantly differ from control. Combined exposure led to increased oxidative stress responses in 3- and 7-day treatments, compared to single IRI. Single IRI caused the highest DNA damage at all timepoints. Continuous 7-day oral exposure to single THC caused an increased mean value of comet tail length compared to its shorter treatments. Concomitant intake of THC slightly affected the levels of IRI genotoxicity at all timepoints, but not in a consistent manner. Further studies are needed to prove our preliminary observations, clarify the underlying mechanisms behind IRI and THC interactions, and unambiguously confirm or reject the assumptions made herein.
Aromaticity as a Guiding Concept for Spectroscopic Features and Nonlinear Optical Properties of Porphyrinoids
Authors: Woller Tatiana , Geerlings Paul , De Proft Frank , Champagne Benoît , Alonso Mercedes
Published: June 2018

With their versatile molecular topology and aromaticity, porphyrinoid systems combine remarkable chemistry with interesting photophysical properties and nonlinear optical properties. Hence, the field of application of porphyrinoids is very broad ranging from near-infrared dyes to opto-electronic materials. From previous experimental studies, aromaticity emerges as an important concept in determining the photophysical properties and two-photon absorption cross sections of porphyrinoids. Despite a considerable number of studies on porphyrinoids, few investigate the relationship between aromaticity, UV/vis absorption spectra and nonlinear properties. To assess such structure-property relationships, we performed a computational study focusing on a series of Hückel porphyrinoids to: (i) assess their (anti)aromatic character; (ii) determine the fingerprints of aromaticity on the UV/vis spectra; (iii) evaluate the role of aromaticity on the NLO properties. Using an extensive set of aromaticity descriptors based on energetic, magnetic, structural, reactivity and electronic criteria, the aromaticity of [4n+2] π-electron porphyrinoids was evidenced as was the antiaromaticity for [4n] π-electron systems. In agreement with previous studies, the absorption spectra of aromatic systems display more intense B and Q bands in comparison to their antiaromatic homologues. The nature of these absorption bands was analyzed in detail in terms of polarization, intensity, splitting and composition. Finally, quantities such as the average polarizability and its anisotropy were found to be larger in aromatic systems, whereas first and second hyperpolarizability are influenced by the interplay between aromaticity, planarity and molecular symmetry. To conclude, aromaticity dictates the photophysical properties in porphyrinoids, whereas it is not the only factor determining the magnitude of NLO properties.
Sterepinic Acids A–C, New Carboxylic Acids Produced by a Marine Alga-Derived Fungus
Authors: Yamada Takeshi , Matsuda Miwa , Seki Mayuko , Hirose Megumi , Kikuchi Takashi
Published: June 2018

Sterepinic acids A–C (1–3), new carboxylic acids with two primary alcohols, have been isolated from a fungal strain of Stereum sp. OUPS-124D-1 attached to the marine alga Undaria pinnatifida. Dihydro-1,5-secovibralactone (4), a new vibralactone derivative, was isolated from the same fungal metabolites together with known vibralactone A (5), and 1,5-secovibralactone (6). The planar structures of these compounds have been elucidated by spectroscopic analyses using IR, HRFABMS, and NMR spectra. To determine the absolute configuration of the compounds, we used the phenylglycine methyl ester (PGME) method. These compounds exhibited less activity in the cytotoxicity assay against cancer cell lines.
Synthesis, Antiviral and Cytotoxic Activity of Novel Terpenyl Hybrid Molecules Prepared by Click Chemistry
Authors: Pertino Mariano Walter , Petrera Erina , Alché Laura Edith , Schmeda-Hirschmann Guillermo
Published: June 2018

Naturally occurring terpenes were combined by click reactions to generate sixteen hybrid molecules. The diterpene imbricatolic acid (IA) containing an azide group was used as starting compound for the synthesis of all the derivatives. The alkyne group in the terpenes cyperenoic acid, dehydroabietinol, carnosic acid γ-lactone, ferruginol, oleanolic acid and aleuritolic acid was obtained by esterification using appropriate alcohols or acids. The hybrid compounds were prepared by combining the IA azide function with the different terpene-alkynes under click chemistry conditions. The cytotoxic activity of the terpene hybrids 1–16 was assessed against Vero cells and tumour cell lines (HEP-2, C6 and Raw 264.7). Compounds 1, 2, 3 and 7 showed cytotoxic activity against the tested cell lines. The antiviral activity of the compounds was evaluated against HSV-1 KOS, Field and B2006 strain. For the pairs of hybrid compounds formed between IA-diterpene (compounds 3–8, except for compound 7), a moderate activity was observed against the three HSV-1 strains with an interesting selectivity index (SI ≥10, SI = CC50/CE50) for some compounds.
Easy Employment and Crosstalk-Free Detection of Seven Fluorophores in a Widefield Fluorescence Microscope
Authors: Bhakdi Sebastian , Thaicharoen Ponpan
Published: June 2018

Immunofluorescence staining has become an essential tool in pathology and biomedical sciences to identify rare cells, cell–cell interactions, and submicroscopic cellular components. Many experimental settings, however, suffer from the fact that traditional widefield fluorescence microscopy is usually restricted to imaging three or four fluorophores only. Due to a lack of morphological information and a high detection limit, even flow cytometry—which is capable of staining 20 or more fluorophores at the same time—is limited in its applicability, especially in areas such as rare cell detection. Other advanced imaging approaches, such as confocal laser scanning microscopy and imaging flow cytometry, may be addressing these shortcomings, but in turn require sophisticated downstream data processing and high capital outlay. Here, we describe a new method and filter set-up to routinely employ up to seven fluorophores on a traditional widefield fluorescence microscope equipped with a standard high-pressure mercury light source. Quantification of crosstalk between channels and actual seven-color imaging of cancer cells spiked into leukocytes demonstrate that there is no need for digital compensation correction algorithms. Our set-up thus permits a detailed analysis of rare cell populations, co-localization of antigens, and cell morphology in a standard research or routine laboratory setting.
Establishment of Orthotopic Liver Tumors by Surgical Intrahepatic Tumor Injection in Mice with Underlying Non-Alcoholic Fatty Liver Disease
Authors: Brown Zachary J. , Heinrich Bernd , Greten Tim F.
Published: June 2018

The prevalence of non-alcoholic fatty liver disease (NAFLD) and its advanced form, nonalcoholic steatohepatitis (NASH), is increasing, and as such its contribution to the development of hepatocellular carcinoma is also rising. NAFLD has been shown to influence the immune tumor microenvironment. Therefore, development of pre-clinical mouse models in the context of NAFLD are increasingly important. Here, we describe a mouse model designed to recapitulate the findings of NAFLD followed by rapid induction of orthotopic liver tumors with intrahepatic tumor injection. Additionally, we utilized bioluminescent imaging to monitor tumor growth and response to therapy. The development of one dominant tumor nodule allows precise separation of tumor and liver tissue. This is useful for immunotherapy studies as mononuclear cells from the tumor and the surrounding liver tissue can be analyzed separately.
Nanoporous Ni with High Surface Area for Potential Hydrogen Storage Application
Authors: Zhou Xiaocao , Zhao Haibo , Fu Zhibing , Qu Jing , Zhong Minglong , Yang Xi , Yi Yong , Wang Chaoyang
Published: June 2018

Nanoporous metals with considerable specific surface areas and hierarchical pore structures exhibit promising applications in the field of hydrogen storage, electrocatalysis, and fuel cells. In this manuscript, a facile method is demonstrated for fabricating nanoporous Ni with a high surface area by using SiO2 aerogel as a template, i.e., electroless plating of Ni into an SiO2 aerogel template followed by removal of the template at moderate conditions. The effects of the prepared conditions, including the electroless plating time, temperature of the structure, and the magnetism of nanoporous Ni are investigated in detail. The resultant optimum nanoporous Ni with a special 3D flower-like structure exhibited a high specific surface area of about 120.5 m2/g. The special nanoporous Ni exhibited a promising prospect in the field of hydrogen storage, with a hydrogen capacity of 0.45 wt % on 4.5 MPa at room temperature.
Investigating the Synthesis and Characterization of a Novel “Green” H2O2-Assisted, Water-Soluble Chitosan/Polyvinyl Alcohol Nanofiber for Environmental End Uses
Authors: Pervez Md. Nahid , Stylios George K.
Published: June 2018

The present work highlights the formation of a novel green nanofiber based on H2O2-assisted water-soluble chitosan/polyvinyl alcohol (WSCHT/PVA) by using water as an ecofriendly solvent and genipin used as a nontoxic cross-linker. The 20/80 blend ratio was found to have the most optimum uniform fiber morphology. WSCHT retained the same structure as WISCHT. The prepared nanofibers were characterized by Scanning electron microscopy (SEM), Fourier transform spectroscopy (FTIR), Thermo gravimetric analysis (TGA), Differential scanning calorimeter (DSC), X-ray diffraction (XRD), Water Contact Angle (WCA) and Ultraviolet-visible spectroscopy (UV-vis). During electrospinning, the crystalline microstructure of the WSCHT/PVA underwent better solidification and after cross-linking there was an increase in the melting temperature of the fiber. Swelling ratio studies revealed noticeable increase in hydrophilicity with increase of WSCHT, which was further demonstrated by the decrease of contact angle from 64.74° to 14.68°. WSCHT/PVA nanofiber mats exhibit excellent UV blocking protection with less than 5% transmittance value and also showed improved in vitro drug release properties with stable release for longer duration (cross-linked fibers) and burst release for shorter duration (uncross linked) fibers. Finally our experimental data demonstrates excellent adsorption ability of Colour Index (C.I.) reactive black 5 (RB5) due to protonated amino groups.
Biocompatible Gold Nanoparticles Ameliorate Retinoic Acid-Induced Cell Death and Induce Differentiation in F9 Teratocarcinoma Stem Cells
Authors: Gurunathan Sangiliyandi , Kim Jin-Hoi
Published: June 2018

The unique properties of gold nanoparticles (AuNPs) have attracted much interest for a range of applications, including biomedical applications in the cosmetic industry. The current study assessed the anti-oxidative effect of AuNPs against retinoic acid (RA)-induced loss of cell viability; cell proliferation; expression of oxidative and anti-oxidative stress markers, pro- and anti-apoptotic genes, and differentiation markers; and mitochondrial dysfunction in F9 teratocarcinoma stem cells. AuNPs were prepared by reduction of gold salts using luteolin as a reducing and stabilizing agent. The prepared AuNPs were spherical in shape with an average diameter of 18 nm. F9 cells exposed to various concentrations of these AuNPs were not harmed, whereas cells exposed to RA exhibited a dose-dependent change in cell viability and cell proliferation. The RA-mediated toxicity was associated with increased leakage of lactate dehydrogenase, reactive oxygen species, increased levels of malondialdehyde and nitric oxide, loss of mitochondrial membrane potential, and a reduced level of ATP. Finally, RA increased the level of pro-apoptotic gene expression and decreased the expression of anti-apoptotic genes. Interestingly, the toxic effect of RA appeared to be decreased in cells treated with RA in the presence of AuNPs, which was coincident with the increased levels of anti-oxidant markers including thioredoxin, glutathione peroxidases, glutathione, glutathione disulfide, catalase, and superoxide dismutase. Concomitantly, AuNPs ameliorated the apoptotic response by decreasing the mRNA expression of p53, p21, Bax, Bak, caspase-3, caspase-9, and increasing the expressions of Bcl-2 and Bcl-Xl. Interestingly, AuNPs not only ameliorated oxidative stress but also induced differentiation in F9 cells by increasing the expression of differentiation markers including retinoic acid binding protein, laminin 1, collagen type IV, and Gata 6 and decreasing the expressions of markers of stem cell pluripotency including Nanog, Rex1, octamer-binding transcription factor 4, and Sox-2. These consistent cellular and biochemical data suggest that AuNPs could ameliorate RA-induced cell death and facilitate F9 cell differentiation. AuNPs could be suitable therapeutic agents for the treatment of oxidative stress-related diseases such as atherosclerosis, cancer, diabetes, rheumatoid arthritis, and neurodegenerative diseases.
WAVE~Ripples for Change Obesity Two-Year Intervention in High School Soccer Players: Process Evaluation, Best Practices, and Youth Engagement
Authors: Meng Yu , Wong Siew Sun , Manore Melinda M. , Patton-López Mēgan
Published: June 2018

This paper reports the process data on program fidelity, best practices for intervention implementation, youth and coach engagement, and youth application of knowledge and skills for the two-year WAVE~Ripples for Change (WAVE) obesity prevention intervention program focused on healthy eating, physical activity, and life skills with high school (HS) soccer players aged 14–19 years. Internal (staff: n = 7; volunteers: n = 27) and external (youth: n = 100; coaches: n = 9) stakeholders were interviewed/ surveyed. Staff rated program fidelity as high (94%), as did volunteers (85%). Best practices included coach encouragement for athlete participation, use of on-line consent for enrollment, building relationships with HS staff to complete assessments, sending text reminders, and providing incentives. Study results showed an enrollment rate of 72%, completion of baseline assessments of 89–98%, attendance of sports nutrition lessons in Year 1 and Year 2 of 90% and 39%, respectively, and team-building workshop (TBW) attendance of 25–31%. Activities exceeding youth expectations (>90%) included, (1) activities with their soccer team; (2) the TBW-cooking; and (3) sports nutrition lessons. The obesity prevention skills most applied by youth were obtained from the TBW-gardening and harvesting (49%), the TBW-cooking (43%), and sports nutrition lessons (44%). Coaches also rated the sports nutrition lessons highly and reported increased awareness for hydration/fueling during sport by the athletes. Using sport teams/clubs to engage youth in obesity prevention is a feasible model for future study.
Ferulic Acid Supplementation Improves Lipid Profiles, Oxidative Stress, and Inflammatory Status in Hyperlipidemic Subjects: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Bumrungpert Akkarach , Lilitchan Supathra , Tuntipopipat Siriporn , Tirawanchai Nednapis , Komindr Surat
Published: June 2018

Ferulic acid is the most abundant phenolic compound found in vegetables and cereal grains. In vitro and animal studies have shown ferulic acid has anti-hyperlipidemic, anti-oxidative, and anti-inflammatory effects. The objective of this study is to investigate the effects of ferulic acid supplementation on lipid profiles, oxidative stress, and inflammatory status in hyperlipidemia. The study design is a randomized, double-blind, placebo-controlled trial. Subjects with hyperlipidemia were randomly divided into two groups. The treatment group (n = 24) was given ferulic acid (1000 mg daily) and the control group (n = 24) was provided with a placebo for six weeks. Lipid profiles, biomarkers of oxidative stress and inflammation were assessed before and after the intervention. Ferulic acid supplementation demonstrated a statistically significant decrease in total cholesterol (8.1%; p = 0.001), LDL-C (9.3%; p < 0.001), triglyceride (12.1%; p = 0.049), and increased HDL-C (4.3%; p = 0.045) compared with the placebo. Ferulic acid also significantly decreased the oxidative stress biomarker, MDA (24.5%; p < 0.001). Moreover, oxidized LDL-C was significantly decreased in the ferulic acid group (7.1%; p = 0.002) compared with the placebo group. In addition, ferulic acid supplementation demonstrated a statistically significant reduction in the inflammatory markers hs-CRP (32.66%; p < 0.001) and TNF-α (13.06%; p < 0.001). These data indicate ferulic acid supplementation can improve lipid profiles and oxidative stress, oxidized LDL-C, and inflammation in hyperlipidemic subjects. Therefore, ferulic acid has the potential to reduce cardiovascular disease risk factors.
Modulation of Free Amino Acid Profile in Healthy Humans Administered with Mastiha Terpenes. An Open-Label Trial
Authors: Papada Efstathia , Torović Ljilja , Amerikanou Charalampia , Kalogeropoulos Nikolaos , Smyrnioudis Ilias , Kaliora Andriana C.
Published: June 2018

We aimed to explore whether plasma-free amino acids are modified in response to terpenes administration in healthy humans. In this open-label, single-arm acute trial, seventeen healthy male volunteers were administered with a naturally occurring product of known terpenes—namely mastiha—after overnight fasting. Blood samples were collected at different time points before and after ingestion. We aimed at identifying and quantifying 60 free amino acids in plasma applying Gas Chromatography-Mass Spectrometry. A total of 24 free amino acids were quantified. Branched-chain valine significantly decreased 4 h post-ingestion, whereas proline decreased at 6 h and ornithine at 2 h, compared to 0 h. These novel findings demonstrate that free amino acids levels are modulated in response to terpenes intake in healthy subjects.
Behavioral Correlates of Empirically-Derived Dietary Patterns among University Students
Authors: Mueller Megan P. , Blondin Stacy A. , Korn Ariella R. , Bakun Peter J. , Tucker Katherine L. , Economos Christina D.
Published: June 2018

Given the importance of young adulthood in establishing lifelong dietary habits, it is imperative to better understand potential underlying drivers of dietary behavior in the university-age population. Dietary patterns have been associated with disease risk, but behavioral predictors of dietary pattern adherence are poorly understood, especially among emerging adults. This study aims to evaluate health-related behaviors associated with dietary pattern scores among freshmen participating in the Tufts Longitudinal Health Study (TLHS; n = 630). We previously derived dietary patterns using principal components analysis and orthogonal rotation from dietary intake data. Health-related behavior data were collected via survey. All data were collected during the Spring semesters of 1998–2007. Unadjusted linear models were used to determine associations between dietary pattern scores and health-related behaviors. Significant correlates were retained in multivariable regression models, which were adjusted for demographic characteristics. We found that never eating meals away from home was associated with higher adherence to the Prudent and lower adherence to the Western and Alcohol patterns. Intention to lose weight was negatively associated with the Western pattern, while intention to gain weight was positively associated with all dietary patterns. These findings suggest that intervention efforts aimed at improving eating out behaviors and engaging in healthy weight management strategies may promote healthier dietary patterns among university students.
Combining Short-Term Interval Training with Caloric Restriction Improves ß-Cell Function in Obese Adults
Authors: Francois Monique E. , Gilbertson Nicole M. , Eichner Natalie Z. M. , Heiston Emily M. , Fabris Chiara , Breton Marc , Mehaffey J. Hunter , Hassinger Taryn , Hallowell Peter T. , Malin Steven K.
Published: June 2018

Although low-calorie diets (LCD) improve glucose regulation, it is unclear if interval exercise (INT) is additive. We examined the impact of an LCD versus LCD + INT training on ß-cell function in relation to glucose tolerance in obese adults. Twenty-six adults (Age: 46 ± 12 year; BMI 38 ± 6 kg/m2) were randomized to 2-week of LCD (~1200 kcal/day) or energy-matched LCD + INT (60 min/day alternating 3 min at 90 and 50% HRpeak). A 2 h 75 g oral glucose tolerance test (OGTT) was performed. Insulin secretion rates (ISR) were determined by deconvolution modeling to assess glucose-stimulated insulin secretion ([GSIS: ISR/glucose total area under the curve (tAUC)]) and ß-cell function (Disposition Index [DI: GSIS/IR]) relative to skeletal muscle (Matsuda Index), hepatic (HOMA-IR) and adipose (Adipose-IRfasting) insulin resistance (IR). LCD + INT, but not LCD alone, reduced glucose and total-phase ISR tAUC (Interactions: p = 0.04 and p = 0.05, respectively). Both interventions improved skeletal muscle IR by 16% (p = 0.04) and skeletal muscle and hepatic DI (Time: p < 0.05). Improved skeletal muscle DI was associated with lower glucose tAUC (r = −0.57, p < 0.01). Thus, LCD + INT improved glucose tolerance more than LCD in obese adults, and these findings relate to ß-cell function. These data support LCD + INT for preserving pancreatic function for type 2 diabetes prevention.
Induction of Apoptosis and Cytotoxicity by Isothiocyanate Sulforaphene in Human Hepatocarcinoma HepG2 Cells
Authors: Kntayya Saie Brindha , Ibrahim Muhammad Din , Mohd Ain Nooraini , Iori Renato , Ioannides Costas , Abdull Razis Ahmad Faizal
Published: June 2018

Glucoraphenin, a glucosinolate present in large quantities in radish is hydrolysed by myrosinase to form the isothiocyanate sulforaphene, which is believed to be responsible for its chemopreventive activity; however, the underlying mechanisms of action have not been investigated, particularly in human cell lines. The aim of the study is to assess the cytotoxicity of sulforaphene in HepG2 cells and evaluate its potential to enhance apoptosis. The cytotoxicity of sulforaphene in HepG2 cells was carried out ensuing an initial screening with two other cell lines, MFC-7 and HT-29, where sulforaphene displayed highest toxicity in HepG2 cells following incubation at 24, 48 and 72 h. In contrast, the intact glucosinolate showed no cytotoxicity. Morphological studies indicated that sulforaphene stimulated apoptosis as exemplified by cell shrinkage, blebbing, chromatin condensation, and nuclear fragmentation. The Annexin V assay revealed significant increases in apoptosis and the same treatment increased the activity of caspases -3/7 and -9, whereas a decline in caspase-8 was observed. Impairment of cell proliferation was indicated by cell cycle arrest at the Sub G0/G1 phase as compared to the other phases. It may be concluded that sulforaphene, but not its parent glucosinolate, glucoraphenin, causes cytotoxicity and stimulates apoptosis in HepG2 cells.
Preparation, Characterization and Wound Healing Effects of New Membranes Based on Chitosan, Hyaluronic Acid and Arginine Derivatives
Authors: Iacob Andreea-Teodora , Drăgan Maria , Ghețu Nicolae , Pieptu Dragoș , Vasile Cornelia , Buron Frédéric , Routier Sylvain , Giusca Simona , Caruntu Irina-Draga , Profire Lenuța
Published: June 2018

New membranes based on chitosan and chitosan-hyaluronic acid containing new arginine derivatives with thiazolidine-4-one scaffold have been prepared using the ionic cross-linking method. The presence of the arginine derivatives with thiazolidine-4-one scaffold into the polymer matrix was proved by Fourier-transform infrared spectroscopy (FT-IR). The scanning electron microscopy (SEM) revealed a micro-porous structure that is an important characteristic for the treatment of burns, favoring the exudate absorption, the rate of colonization, the cell structure, and the angiogenesis process. The developed polymeric membranes also showed good swelling degree, improved hydrophilicity, and biocompatibility in terms of surface free energy components, which supports their application for tissue regeneration. Moreover, the chitosan-arginine derivatives (CS-6h, CS-6i) and chitosan-hyaluronic acid-arginine derivative (CS-HA-6h) membranes showed good healing effects on the burn wound model induced to rats. For these membranes a complete reepithelialization was observed after 15 days of the experiment, which supports a faster healing process.
Synthetic Glycopolypeptide Micelle for Targeted Drug Delivery to Hepatic Carcinoma
Authors: Li Pengqiang , Han Jiandong , Li Di , Chen Jinjin , Wang Wei , Xu Weiguo
Published: June 2018

The targeted delivery of chemotherapy drugs to tumor lesions is a major challenge for the treatment of tumors. Up until now, various polymeric nanoparticles have been explored to improve the targetability of these therapeutic drugs through passive or active targeting processes. In the design and construction of polymer nanoparticles, glycopolypeptide has shown great potential owing to its excellent targeting ability and biocompatibility. In order to enhance the antitumor effect of doxorubicin (DOX), a glycopolypeptide-based micelle (GPM) modified by α-lactose (Lac) was synthesized for targeted treatment of hepatoma. The DOX-loaded GPM (i.e., GPM/DOX) could significantly target human hepatoma (HepG2) cells and further inhibit their proliferation in vitro. Additionally, GPM/DOX exhibited a much higher drug accumulation in tumor tissue and a stronger antitumor effect in vivo than free DOX. The above results revealed that this drug delivery system provides a promising platform for the targeting therapy of hepatic cancer.
Chirality Construction from Preferred π-π Stacks of Achiral Azobenzene Units in Polymer: Chiral Induction, Transfer and Memory
Authors: Miao Tengfei , Yin Lu , Cheng Xiaoxiao , Zhao Yin , Hou Wenjie , Zhang Wei , Zhu Xiulin
Published: June 2018

The induction of supramolecular chirality from achiral polymers has been widely investigated in composite systems consisting of a chiral guest, achiral host, and solvents. To further study and understand the process of chirality transfer from a chiral solvent or chiral molecules to an achiral polymer backbone or side-chain units, an alternative is to reduce the components in the supramolecular assembled systems. Herein, achiral side-chain azobenzene (Azo)-containing polymers, poly(6-[4-(4-methoxyphenylazo) phenoxy] hexyl methacrylate) (PAzoMA), with different Mns, were synthesized by atom transfer radical polymerization (ATRP). Preferred chirality from supramolecular assembled trans-Azo units of PAzoMAs is successfully induced solely by the neat limonene. These aggregates of the polymers in limonene solution were characterized by circular dichroism (CD), UV-vis spectra, and dynamic light scattering (DLS) under different temperatures. The temperature plays an important role in the course of chiral induction. Meanwhile, supramolecular chirality can be constructed in the solid films of the achiral side-chain Azo-containing polymers that were triggered by limonene vapors. Also, it can be erased after heated above the glass transition temperature (Tg) of the polymer, and recovered after cooling down in the limonene vapors. A chiroptical switch can be built by alternately changing the temperature. The solid films show good chiral memory behaviors. The current results will facilitate studying the mechanism of chirality transfer induced by chiral solvent and improve potential application possibilities in chiral film materials.
Preparation and Characterization of Regenerated Cellulose Film from a Solution in Lithium Bromide Molten Salt Hydrate
Authors: Zhang Xueqin , Xiao Naiyu , Wang Huihui , Liu Chuanfu , Pan Xuejun
Published: June 2018

In this study, the molten salt hydrate of lithium bromide (LiBr) was utilized as a non-derivatizing cellulose dissolution solvent to prepare regenerated cellulose films for kraft pulp. The effects of LiBr concentrations (60, 62, and 65 wt %) and dissolving time (from 5 to 40 min with the interval of 5 min) on the structures and the properties of the films were investigated. Fourier transform infrared (FT-IR) and cross-polarization magic-angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C NMR) characterizations verified the breakage of inter- and intra-cellulose hydrogen bonds during the regeneration, resulting in the disruption of the crystalline structure of cellulose. X-ray diffraction (XRD) data indicated that the regeneration converted the polymorphism of cellulose from I to II as well as decreased its crystallinity. Ultraviolet-visible spectra (UV-Vis) and scanning electron microscopy (SEM) analyses revealed the excellent optical transparency of the films to visible light due to the complete dissolution of cellulose fibers as well as the sufficient breaking of the inter- and intra-cellulose hydrogen bonds. In terms of tensile testing, tuning LiBr concentrations and dissolving time could increase the elongation at break and tensile strength of the films. The maximum elongation at break of 26% and tensile strength of 67 MPa were achieved when the films prepared in 65 wt % LiBr for 10 and 15 min, respectively. These results indicated the great potential of the cellulose films for packaging use.
Large Scale Proteomic Data and Network-Based Systems Biology Approaches to Explore the Plant World
Authors: Di Silvestre Dario , Bergamaschi Andrea , Bellini Edoardo , Mauri PierLuigi
Published: June 2018

The investigation of plant organisms by means of data-derived systems biology approaches based on network modeling is mainly characterized by genomic data, while the potential of proteomics is largely unexplored. This delay is mainly caused by the paucity of plant genomic/proteomic sequences and annotations which are fundamental to perform mass-spectrometry (MS) data interpretation. However, Next Generation Sequencing (NGS) techniques are contributing to filling this gap and an increasing number of studies are focusing on plant proteome profiling and protein-protein interactions (PPIs) identification. Interesting results were obtained by evaluating the topology of PPI networks in the context of organ-associated biological processes as well as plant-pathogen relationships. These examples foreshadow well the benefits that these approaches may provide to plant research. Thus, in addition to providing an overview of the main-omic technologies recently used on plant organisms, we will focus on studies that rely on concepts of module, hub and shortest path, and how they can contribute to the plant discovery processes. In this scenario, we will also consider gene co-expression networks, and some examples of integration with metabolomic data and genome-wide association studies (GWAS) to select candidate genes will be mentioned.
A Case Study for a New Peer-Review Journal on Race and Ethnicity in American Higher Education
Authors: Salinas Cristobal
Published: June 2018

In this exploratory case study, the interests, attitudes, and opinions of participants of the National Conference on Race and Ethnicity (NCORE) in American Higher Education are presented. This case study sought to understand how college and university administrators and faculty perceived the need to create a peer-reviewed journal that aimed to support and create opportunities to publish research, policy, practices, and procedures within the context of race and ethnicity in American higher education. The findings of this study reflect that the vast majority of those surveyed (n = 605) and interviewed (n = 5) support, and are interested in, having a peer-reviewed journal that focuses on race and ethnicity in American higher education.
The Potential Phosphorus Crisis: Resource Conservation and Possible Escape Technologies: A Review
Authors: Daneshgar Saba , Callegari Arianna , Capodaglio Andrea G. , Vaccari David
Published: June 2018

Phosphorus is an essential nutrient for every organism on the Earth, yet it is also a potential environmental pollutant, which may cause eutrophication of water bodies. Wastewater treatment plants worldwide are struggling to eliminate phosphorus from effluents, at great cost, yet current research suggests that the world may deplete the more available phosphorus reserves by around 2300. This, in addition to environmental concerns, evokes the need for new phosphorus recovery techniques to be developed, to meet future generations needs for renewable phosphorus supply. Many studies have been, and are, carried out on phosphorus recovery from wastewater and its sludge, due to their high phosphorus content. Chemical precipitation is the main process for achieving a phosphorus-containing mineral suitable for reuse as a fertilizer, such as struvite. This paper reviews the current status and future trends of phosphorus production and consumption, and summarizes current recovery technologies, discussing their possible integration into wastewater treatment processes, according to a more sustainable water-energy-nutrient nexus.
Focusing High-Resolution Highly-Squinted Airborne SAR Data with Maneuvers
Authors: Tang Shiyang , Zhang Linrang , So Hing Cheung
Published: June 2018

Maneuvers provide flexibility for high-resolution highly-squinted (HRHS) airborne synthetic aperture radar (SAR) imaging and also mean complex signal properties in the echoes. In this paper, considering the curved path described by the fifth-order motion parameter model, effects of the third- and higher-order motion parameters on imaging are analyzed. The results indicate that the spatial variations distributed in range, azimuth, and height directions, have great impacts on imaging qualities, and they should be eliminated when designing the focusing approach. In order to deal with this problem, the spatial variations are decomposed into three main parts: range, azimuth, and cross-coupling terms. The cross-coupling variations are corrected by polynomial phase filter, whereas the range and azimuth terms are removed via Stolt mapping. Different from the traditional focusing methods, the cross-coupling variations can be removed greatly by the proposed approach. Implementation considerations are also included. Simulation results prove the effectiveness of the proposed approach.
The Application of Discrete Wavelet Transform with Improved Partial Least-Squares Method for the Estimation of Soil Properties with Visible and Near-Infrared Spectral Data
Authors: Wang Guoqiang , Wang Wei , Fang Qingqing , Jiang Hong , Xin Qinchuan , Xue Baolin
Published: June 2018

This study evaluated whether wavelet functions (Bior1.3, Bior2.4, Db4, Db8, Haar, Sym4, and Sym8) and decomposition levels (Levels 3–8) can estimate soil properties. The analysis is based on the discrete wavelet transform with partial least-squares (DWT–PLS) method, incorporated into a visible and near-infrared reflectance analysis. The improved DWT–PLS method (called DWT–Stepwise-PLS) enhances the accuracy of the quantitative analysis model with DWT–PLS. The cation exchange capacity (CEC) was best estimated by the DWT–PLS model using the Haar wavelet function. This model yielded the highest coefficient of determination (Rv2 = 0.787, p < 0.001), with the highest relative percentage deviation (RPD = 2.047) and lowest root mean square error (RMSE = 4.16) for the validation data set of the CEC. The RPD of the SOM predictions by DWT–PLS using the Bior1.3 wavelet function was maximized at 1.441 (Rv2 = 0.642, RMSE = 5.96), highlighting the poor overall predictive ability of soil organic matter (SOM) by DWT–PLS. Furthermore, the best performing decomposition levels of the wavelet function were distributed in the fifth, sixth, and seventh levels. For various wavelet functions and decomposition levels, the DWT–Stepwise-PLS method more accurately predicted the quantified soil properties than the DWT–PLS model. DWT–Stepwise-PLS using the Haar wavelet function remained the best choice for quantifying the CEC (Rv2 = 0.92, p < 0.001, RMSE = 4.91, and RPD = 3.57), but the SOM was better predicted by DWT–Stepwise-PLS using the Bior2.4 wavelet function (Rv2 = 0.8, RMSE = 5.34, and RPD = 2.24) instead of the Bior1.3 wavelet function. However, the performance of the DWT–Stepwise-PLS method tended to degrade at high and low decomposition levels of the DWT. These degradations were attributed to a lack of sufficient information and noise, respectively.
Monitoring and Characterizing Heterogeneous Mediterranean Landscapes with Continuous Textural Indices Based on VHSR Imagery
Authors: Lang Marc , Alleaume Samuel , Luque Sandra , Baghdadi Nicolas , Féret Jean-Baptiste
Published: June 2018

Remote sensing tools (RS) can contribute to a better understanding of the diversity of natural and semi-naturals habitats, their spatial distribution, and their conservation status. RS can also provide a generic set of derived indicators to support local to regional habitat monitoring. Here we propose a set of synthetic continuous textural indices computed from high spatial resolution airborne images for the characterization of vegetation structure in very heterogeneous landscape mosaics. These indices are based on Fourier-based textural ordination (FOTO) of very-high-resolution images. We investigate the relationship between textural indices and a set of common landscape metrics derived from vegetation maps, identifying four strata of interest: bare soil, herbs, low ligneous, and high ligneous. We identify two continuous textural indices, the first one being related to vegetation strata fragmentation and the second being related to the dominance of high ligneous. The combination of these two textural indices with the Normalized Difference Vegetation Index (NDVI) provides a synoptic and accurate overview of the spatial organization of the different vegetation strata. The methodological approach presented herein has a generic value in response to national conservation targets in the context of mapping relevant habitat indicators.
Low-Frequency Sea Surface Radar Doppler Echo
Authors: Yurovsky Yury Yu. , Kudryavtsev Vladimir N. , Grodsky Semyon A. , Chapron Bertrand
Published: June 2018

The sea surface normalized radar backscatter cross-section (NRCS) and Doppler velocity (DV) exhibit energy at low frequencies (LF) below the surface wave peak. These NRCS and DV variations are coherent and thus may produce a bias in the DV averaged over large footprints, which is important for interpretation of Doppler scatterometer measurements. To understand the origin of LF variations, the platform-borne Ka-band radar measurements with well-pronounced LF variations at frequencies below wave peak (0.19 Hz) are analyzed. These data show that the LF NRCS is coherent with wind speed at 21 m height while the LF DV is not. The NRCS-wind correlation is significant only at frequencies below 0.01 Hz indicating either differences between near-surface wind (affecting radar signal) and 21-m height wind (actually measured) or contributions of other mechanisms of LF radar signal variations. It is shown that non-linearity in NRCS-wave slope Modulation Transfer Function (MTF) and inherent averaging within radar footprint account for NRCS and DV LF variance, with the exception of VV NRCS for which almost half of the LF variance is unexplainable by these mechanisms and perhaps attributable to wind fluctuations. Although the distribution of radar DV is quasi-Gaussian, suggesting virtually little impact of non-linearity, the LF DV variations arise due to footprint averaging of correlated local DV and non-linear NRCS. Numerical simulations demonstrate that MTF non-linearity weakly affects traditional linear MTF estimate (less than 10% for typical MTF magnitudes less than 20). Thus the linear MTF is a good approximation to evaluate the DV averaged over large footprints typical of satellite observations.
Energy Harvesting over Rician Fading Channel: A Performance Analysis for Half-Duplex Bidirectional Sensor Networks under Hardware Impairments
Authors: Nguyen Tan N. , Quang Minh Tran Hoang , Tran Phuong T. , Vozňák Miroslav
Published: June 2018

In this paper, a rigorous analysis of the performance of time-switching energy harvesting strategy that is applied for a half-duplex bidirectional wireless sensor network with intermediate relay over a Rician fading channel is presented to provide the exact-form expressions of the outage probability, achievable throughput and the symbol-error-rate (SER) of the system under the hardware impairment condition. Using the proposed probabilistic models for wireless channels between mobile nodes as well as for the hardware noises, we derive the outage probability of the system, and then the throughput and SER can be obtained as a result. Both exact analysis and asymptotic analysis at high signal-power-to-noise-ratio regime are provided. Monte Carlo simulation is also conducted to verify the analysis. This work confirms the effectiveness of energy harvesting applied in wireless sensor networks over a Rician fading channel, and can provide an insightful understanding about the effect of various parameters on the system performance.
A Survey on Proactive, Active and Passive Fault Diagnosis Protocols for WSNs: Network Operation Perspective
Authors: Mehmood Amjad , Alrajeh Nabil , Mukherjee Mithun , Abdullah Salwani , Song Houbing
Published: June 2018

Although wireless sensor networks (WSNs) have been the object of research focus for the past two decades, fault diagnosis in these networks has received little attention. This is an essential requirement for wireless networks, especially in WSNs, because of their ad-hoc nature, deployment requirements and resource limitations. Therefore, in this paper we survey fault diagnosis from the perspective of network operations. To the best of our knowledge, this is the first survey from such a perspective. We survey the proactive, active and passive fault diagnosis schemes that have appeared in the literature to date, accenting their advantages and limitations of each scheme. In addition to illuminating the details of past efforts, this survey also reveals new research challenges and strengthens our understanding of the field of fault diagnosis.
A Silicon-on-Insulator-Based Dual-Gain Charge-Sensitive Pixel Detector for Low-Noise X-ray Imaging for Future Astronomical Satellite Missions
Authors: Shrestha Sumeet , Kawahito Shoji , Kamehama Hiroki , Nakanishi Syunta , Yasutomi Keita , Kagawa Keiichiro , Teranishi Nobukazu , Takeda Ayaki , Tsuru Takeshi Go , Kurachi Ikuo , Arai Yasuo
Published: June 2018

In this paper, we report on the development of a monolithic active pixel sensor for X-ray imaging using 0.2 µm fully depleted silicon-on-insulator (SOI)-based technology to support next generation astronomical satellite missions. Detail regarding low-noise dual-gain SOI based pixels with a charge sensitive amplifier and pinned depleted diode sensor structure is presented. The proposed multi-well sensor structure underneath the fully-depleted SOI allows the design of a detector with low node capacitance and high charge collection efficiency. Configurations for achieving very high charge-to-voltage conversion gain of 52 µV/e− and 187 µV/e− are demonstrated. Furthermore, in-pixel dual gain selection is used for low-noise and wide dynamic range X-ray energy detection. A technique to improve the noise performance by removing correlated system noise leads to an improvement in the spectroscopic performance of the measured X-ray energy. Taken together, the implemented chip has low dark current (44.8 pA/cm2 at −30 °C), improved noise performance (8.5 e− rms for high gain and 11.7 e− rms for low gain), and better energy resolution of 2.89% (171 eV FWHM) at 5.9 keV using 55Fe and 1.67% (234 eV FWHM) at 13.95 keV using 241Am.
Development of a Fault Monitoring Technique for Wind Turbines Using a Hidden Markov Model
Authors: Shin Sung-Hwan , Kim SangRyul , Seo Yun-Ho
Published: June 2018

Regular inspection for the maintenance of the wind turbines is difficult because of their remote locations. For this reason, condition monitoring systems (CMSs) are typically installed to monitor their health condition. The purpose of this study is to propose a fault detection algorithm for the mechanical parts of the wind turbine. To this end, long-term vibration data were collected over two years by a CMS installed on a 3 MW wind turbine. The vibration distribution at a specific rotating speed of main shaft is approximated by the Weibull distribution and its cumulative distribution function is utilized for determining the threshold levels that indicate impending failure of mechanical parts. A Hidden Markov model (HMM) is employed to propose the statistical fault detection algorithm in the time domain and the method whereby the input sequence for HMM is extracted is also introduced by considering the threshold levels and the correlation between the signals. Finally, it was demonstrated that the proposed HMM algorithm achieved a greater than 95% detection success rate by using the long-term signals.