bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamical Filtering Highlights the Seasonality of Surface-Balanced Motions at Diurnal Scales in the Eastern Boundary Currents
Authors: Quintana Antonio , Torres Hector S. , Gomez-Valdes Jose
Published: August 2022

Balanced motions (BM) and internal gravity waves (IGW) account for most of the kinetic energy budget and capture most of the vertical velocity in the ocean. However, estimating the contribution of BM to both issues at time scales of less than a day is a challenge because BM are obscured by IGW. To study the BM regime, we outlined the implementation of a dynamical filter that separates both classes of motion. This study used a high-resolution global simulation to analyze the Eastern Boundary Currents during the winter and summer months. Our results confirm the feasibility of recovering BM dynamics at short time scales, emphasizing the diurnal cycle in winter and its dampening in summer due to local stratification that prevents large vertical excursion of the surface boundary layer. Our filter opens up new possibilities for more accurate estimation of the vertical exchanges of any tracers at any vertical level in the water column. Moreover, it could be a valuable tool for studies focused on wave–turbulence interactions in ocean simulations.
The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts
Authors: Pierfelice Tania Vanessa , D’Amico Emira , Petrini Morena , Pandolfi Assunta , D’Arcangelo Camillo , Di Pietro Natalia , Piattelli Adriano , Iezzi Giovanna
Published: August 2022

This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT), consisting of 5% 5-aminolevulinic acid-gel and 630 nm-LED, already used for antibacterial effects in the treatment of periodontitis, on human gingival fibroblasts (HGF) and primary human osteoblasts (HOB). HGF and HOB were incubated with different ALAD concentrations for 45 min, and subsequently irradiated with 630 nm-LED for 7 min. Firstly, the cytotoxicity at 24 h and proliferation at 48 and 72 h were assessed. Then the intracellular content of the protoporphyrin IX (PpIX) of the ROS and the superoxide dismutase (SOD) activity were investigated at different times. Each result was compared with untreated and unirradiated cells as the control. Viable and metabolic active cells were revealed at any concentrations of ALAD-PDT, but only 100-ALAD-PDT significantly enhanced the proliferation rate. The PpIX fluorescence significantly increased after the addition of 100-ALAD, and decreased after the irradiation. Higher ROS generation was detected at 10 min in HGF, and at 30 min in HOB. The activity of the SOD enzyme augmented at 30 min in both cell types. In conclusion, ALAD-PDT not only showed no cytotoxic effects, but had pro-proliferative effects on HGF and HOB, probably via ROS generation.
Extraction of Palladium(II) with a Magnetic Sorbent Based on Polyvinyl Alcohol Gel, Metallic Iron, and an Environmentally Friendly Polydentate Phosphazene-Containing Extractant
Authors: Yudaev Pavel , Butorova Irina , Stepanov Gennady , Chistyakov Evgeniy
Published: August 2022

In this work, a highly efficient and environmentally friendly method for extracting palladium from hydrochloric acid media was developed. The method uses a magnetic sorbent carrying an organophosphorus extractant, which is not washed from the sorbent into the aqueous phase. The extractant was characterized by 1H, 13C, and 31P NMR spectroscopy and MALDI TOF mass spectrometry, and the palladium complex based on it was characterized by IR spectroscopy. According to an in vitro microbiological study, the extractant was non-toxic to soil microflora. It was established that the water uptake and saturation magnetization of the magnetic sorbent were sufficient for use in sorption processes. The sorption efficiency of palladium(II) with the developed sorbent can reach 71% in one cycle. After treatment of the spent sorbent with 5 M hydrochloric acid, palladium was completely extracted from the sorbent. The new sorbent is proposed for the extraction of palladium from hydrochloric acid media obtained by the leaching of electronic waste.
Incorporation of MyDispense, a Virtual Pharmacy Simulation, into Extemporaneous Formulation Laboratories
Authors: Nicolazzo Joseph A. , Chuang Sara , Mak Vivienne
Published: August 2022

A core competency of Australian Pharmacy graduates is to prepare and compound extemporaneous formulations. Students in our pharmacy course would traditionally formulate extemporaneous products in laboratory classes while simultaneously preparing a handwritten label, with students divorcing this laboratory activity from the entire dispensing process. As a way to incorporate the dispensing process into the preparation of extemporaneous products without adding excessive time to the laboratory, we integrated MyDispense, a virtual pharmacy simulation, in pre-laboratory activities. This meant that students could complete all the dispensing activities for prescribed extemporaneous formulations prior to attending the laboratory. Prescriptions for solutions, suspensions, creams and ointments were developed in MyDispense, including essential components for dispensing an extemporaneous formulation (e.g., formulation name, dosing instructions). These prescriptions were provided to students at least 1 week prior to their laboratory classes, whereas for the laboratory assessments, the prescription was provided at the commencement of the extemporaneous exam. Due to the implementation of dispensing via MyDispense, we found that students demonstrated pre-laboratory engagement as all students presented their printed labels upon entering the laboratory. We also observed an increase in interaction between students and laboratory facilitators, mainly focused on the principles of formulation integrated around patient outcomes. Virtual simulations such as MyDispense can therefore provide a guided realistic learning experience, whilst overcoming time pressures associated with laboratory timetabling. This approach also encourages students to engage in the dispensing process prior to extemporaneous laboratories providing more opportunity to discuss higher-level formulation principles and patient-centred outcomes during laboratory classes.
The Preliminary Efficacy of a Program to Facilitate Benefit Finding for People with Mental Illness: A Pilot Randomized Controlled Trial in Japan
Authors: Chiba Rie , Funakoshi Akiko , Miyamoto Yuki
Published: August 2022

Benefit finding is a concept that refers to finding positive changes or benefits through negative experiences from stressful life events. The present study aimed to develop a new intervention program to facilitate benefit finding for people with mental illness and examine its feasibility and preliminary efficacy from pilot data. We hypothesized that participants who joined the group-based intervention program would show progress in benefit finding, personal recovery, and well-being, as well as alleviated psychiatric symptoms and functional impairment, compared to participants in the control group. The participants in the intervention group joined in a new program which focuses on (1) cognitive–behavioral stress management and (2) own experiences, including what was found or realized through their lives since the onset of mental illness. The program used a workbook comprised of eight 90-min sessions, with one held every week. Twenty-four were found eligible and provided informed consent to participate in the study. About 46% were males, and the average age was 42.5 years. Around 63% were diagnosed with schizophrenia. We did not find significant differences over time by groups. However, medium to large effects in each scale or at least one subscale (i.e., benefit finding, personal recovery, subjective well-being, and psychiatric symptoms and functional impairment) were observed. Future studies with more participants from various settings would be necessary to exactingly examine the effectiveness of the intervention program.
Visualization and Semantic Labeling of Mood States Based on Time-Series Features of Eye Gaze and Facial Expressions by Unsupervised Learning
Authors: Madokoro Hirokazu , Nix Stephanie , Sato Kazuhito
Published: August 2022

This study is intended to develop a stress measurement and visualization system for stress management in terms of simplicity and reliability. We present a classification and visualization method of mood states based on unsupervised machine learning (ML) algorithms. Our proposed method attempts to examine the relation between mood states and extracted categories in human communication from facial expressions, gaze distribution area and density, and rapid eye movements, defined as saccades. Using a psychological check sheet and a communication video with an interlocutor, an original benchmark dataset was obtained from 20 subjects (10 male, 10 female) in their 20s for four or eight weeks at weekly intervals. We used a Profile of Mood States Second edition (POMS2) psychological check sheet to extract total mood disturbance (TMD) and friendliness (F). These two indicators were classified into five categories using self-organizing maps (SOM) and U-Matrix. The relation between gaze and facial expressions was analyzed from the extracted five categories. Data from subjects in the positive categories were found to have a positive correlation with the concentrated distributions of gaze and saccades. Regarding facial expressions, the subjects showed a constant expression time of intentional smiles. By contrast, subjects in negative categories experienced a time difference in intentional smiles. Moreover, three comparative experiment results demonstrated that the feature addition of gaze and facial expressions to TMD and F clarified category boundaries obtained from U-Matrix. We verify that the use of SOM and its two variants is the best combination for the visualization of mood states.
Rapid Polyp Classification in Colonoscopy Using Textural and Convolutional Features
Authors: Lo Chung-Ming , Yeh Yu-Hsuan , Tang Jui-Hsiang , Chang Chun-Chao , Yeh Hsing-Jung
Published: August 2022

Colorectal cancer is the leading cause of cancer-associated morbidity and mortality worldwide. One of the causes of developing colorectal cancer is untreated colon adenomatous polyps. Clinically, polyps are detected in colonoscopy and the malignancies are determined according to the biopsy. To provide a quick and objective assessment to gastroenterologists, this study proposed a quantitative polyp classification via various image features in colonoscopy. The collected image database was composed of 1991 images including 1053 hyperplastic polyps and 938 adenomatous polyps and adenocarcinomas. From each image, textural features were extracted and combined in machine learning classifiers and machine-generated features were automatically selected in deep convolutional neural networks (DCNN). The DCNNs included AlexNet, Inception-V3, ResNet-101, and DenseNet-201. AlexNet trained from scratch achieved the best performance of 96.4% accuracy which is better than transfer learning and textural features. Using the prediction models, the malignancy level of polyps can be evaluated during a colonoscopy to provide a rapid treatment plan.
History and Archaeology in Discourse on the Dernberg–Reconstructing the Historical Landscape of a Medieval Motte-and-Bailey Castle and Deserted Village
Authors: Filzwieser Roland , Ruß David , Kucera Matthias , Doneus Michael , Hasenhündl Gerhard , Verhoeven Geert J. , Zotti Georg , Lenzhofer Andreas , Stüttler Gerhard , Pisz Michał , Neubauer Wolfgang
Published: August 2022

Investigating deserted medieval castles and villages in remote rural areas paired with a scarcity of meaningful written sources is a challenging task that can be significantly enhanced by the use of non-invasive archaeological prospection methods. Furthermore, the interpolation of stratigraphic relationships among maps by analysing paths and field boundaries, as performed by Klaus Schwarz in the 1980s, can also contribute significantly. Thus, in order to resolve numerous unanswered questions, a multidisciplinary approach is required. In this paper we present preliminary archaeological prospection data using magnetics and airborne laser scanning (ALS) as well as methodological considerations on the systematic analysis of historical maps on the site of Dernberg, a medieval motte-and-bailey castle with an adjoining deserted village. The magnetic data and corresponding aerial images, although not providing decisive information on internal structures, show several historical roads that allow for the localisation of the village at the foot of the castle hill, as well as other pathways and defensive structures. Data derived from laser scanning surveys carried out by uncrewed aerial vehicles, respectively, drone flights allow for a significant gain in information compared to publicly available ALS data. In a methodological discussion on the systematic analysis of historical maps, the site of Dernberg is used to illustrate not only how such an analysis can determine chronological sequences with respect to the pattern of former agricultural field systems and road networks, but that these assumptions can be confirmed in part by geophysical surveys.
Does Flash Flood Model Performance Increase with Complexity? Signature and Sensitivity-Based Comparison of Conceptual and Process-Oriented Models on French Mediterranean Cases
Authors: Haruna Abubakar , Garambois Pierre-André , Roux Hélène , Javelle Pierre , Jay-Allemand Maxime
Published: August 2022

We compare three hydrological models of different complexities, GR4H (lumped, continuous), SMASH (distributed, continuous), and MARINE (distributed, event-based), for Mediterranean flash flood modeling. The objective was to understand how differently they simulate the catchment’s behavior, in terms of outlet discharge and internal dynamics, and how these can help to improve the relevance of the models. The methodology involved global sensitivity analysis, calibration/validation, and signature comparison at the event scale with good performances. For all models, we found transfer parameters to be sensitive in the case of Gardon and production parameters in the case of Ardeche. The non-conservative flow component of GR4H was found to be sensitive and could benefit the distributed models. At the event scale, the process-based MARINE model at finer resolution outperformed the two continuous hourly models at flood peak and its timing. SMASH, followed by GR4H, performed better in the volume of water exported. Using the operational surface model SIM2 to benchmark the soil moisture simulated by the three models, MARINE (initialized with SIM1) emerged as the most accurate. GR4H followed closely, while SMASH was the least accurate. Flexible modeling and regionalization should be developed based on multi-source signatures and worldwide physiographic databases.
Chronic-Exposure Low-Frequency Magnetic Fields (Magnetotherapy and Magnetic Stimulation) Influence Serum Serotonin Concentrations in Patients with Low Back Pain—Clinical Observation Study
Authors: Woldańska-Okońska Marta , Koszela Kamil
Published: August 2022

(1) Background: The influence of serotonin on many regulatory mechanisms has not been sufficiently studied. The use of a physical method, assuming the possibility of its action on increasing the concentration of serotonin, may be the direction of therapy limiting the number of antidepressants used. The aim of the research was to study the effects of low-frequency magnetic fields of different characteristics on the circadian profile of serotonin in men with low back pain. (2) Methods: 16 men with back pain syndrome participated in the study. The patients were divided into two groups. In group 1, magnetotherapy (2.9 mT, 40 Hz, square wave, bipolar) was applied at 10.00 a.m. In group 2, the M2P2 magnetic stimulation program of the Viofor JPS device was used. Treatments in each group lasted 3 weeks, 5 days each, with breaks for Saturday and Sunday. The daily serotonin profile was determined the day before the exposure and the day after the last treatment. Blood samples (at night with red light) were collected at 8:00, 12:00, 16:00, 24:00, and 4:00. The patients did not suffer from any chronic or acute disease and were not taking any medications. (3) Results: In group 1, a significant increase in serotonin concentration was observed after 15 treatments at 4:00. In group 2, a significant increase in serotonin concentration was observed at 8:00 after the end of the treatments. In comparison between magnetotherapy and magnetic stimulation, the time points at which differences appeared after the application of serotonin occurred due to the increase in its concentrations after the application of magnetic stimulation. (4) Conclusions: Magnetotherapy and magnetic stimulation, acting in a similar way, increase the concentration of serotonin. Weak magnetic fields work similarly to the stronger ones used in TMS. It is possible to use them in the treatment of mental disorders or other diseases with low serotonin concentrations.
Wastewater Sequencing—An Innovative Method for Variant Monitoring of SARS-CoV-2 in Populations
Authors: Tamáš Michal , Potocarova Alena , Konecna Barbora , Klucar Ľubos , Mackulak Tomas
Published: August 2022

The SARS-CoV-2 outbreak has already affected more than 555 million people, and 6.3 million people have died. Due to its high infectivity, it is crucial to track SARS-CoV-2 outbreaks early to prevent the spread of infection. Wastewater monitoring appears to be a powerful and effective tool for managing epidemiological situations. Due to emerging mutations of SARS-CoV-2, there is a need to monitor mutations in order to control the pandemic. Since the sequencing of randomly chosen individuals is time-consuming and expensive, sequencing of wastewater plays an important role in revealing the dynamics of infection in a population. The sampling method used is a crucial factor and significantly impacts the results. Wastewater can be collected as a grab sample or as a 24 h composite sample. Another essential factor is the sample volume, as is the method of transport used. This review discusses different pretreatment procedures and RNA extraction, which may be performed using various methods, such as column-based extraction, TRIzol, or magnetic extraction. Each of the methods has its advantages and disadvantages, which are described accordingly. RT-qPCR is a procedure that confirms the presence of SARS-CoV-2 genes before sequencing. This review provides an overview of currently used methods for preparing wastewater samples, from sampling to sequencing.
An Integrated Machine Learning Scheme for Predicting Mammographic Anomalies in High-Risk Individuals Using Questionnaire-Based Predictors
Authors: Sun Cheuk-Kay , Tang Yun-Xuan , Liu Tzu-Chi , Lu Chi-Jie
Published: August 2022

This study aimed to investigate the important predictors related to predicting positive mammographic findings based on questionnaire-based demographic and obstetric/gynecological parameters using the proposed integrated machine learning (ML) scheme. The scheme combines the benefits of two well-known ML algorithms, namely, least absolute shrinkage and selection operator (Lasso) logistic regression and extreme gradient boosting (XGB), to provide adequate prediction for mammographic anomalies in high-risk individuals and the identification of significant risk factors. We collected questionnaire data on 18 breast-cancer-related risk factors from women who participated in a national mammographic screening program between January 2017 and December 2020 at a single tertiary referral hospital to correlate with their mammographic findings. The acquired data were retrospectively analyzed using the proposed integrated ML scheme. Based on the data from 21,107 valid questionnaires, the results showed that the Lasso logistic regression models with variable combinations generated by XGB could provide more effective prediction results. The top five significant predictors for positive mammography results were younger age, breast self-examination, older age at first childbirth, nulliparity, and history of mammography within 2 years, suggesting a need for timely mammographic screening for women with these risk factors.
An Overview of the Latest Metabolomics Studies on Atopic Eczema with New Directions for Study
Authors: Afghani Jamie , Traidl-Hoffmann Claudia , Schmitt-Kopplin Philippe , Reiger Matthias , Mueller Constanze
Published: August 2022

Atopic eczema (AE) is an inflammatory skin disorder affecting approximately 20% of children worldwide and early onset can lead to asthma and allergies. Currently, the mechanisms of the disease are not fully understood. Metabolomics, the analysis of small molecules in the skin produced by the host and microbes, opens a window to observe the mechanisms of the disease which then may lead to new drug targets for AE treatment. Here, we review the latest advances in AE metabolomics, highlighting both the lipid and non-lipid molecules, along with reviewing the metabolites currently known to reside in the skin.
Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars
Authors: Deng Dong , Sun Suli , Wu Wenqi , Xiang Chao , Duan Canxing , Yu Dongmei , Wu Xuehong , Zhu Zhendong
Published: August 2022

Induced mutation is useful for improving the disease resistance of various crops. Fusarium wilt and powdery mildew are two important diseases which severely influence pea production worldwide. In this study, we first evaluated Fusarium wilt and powdery mildew resistance of mutants derived from two elite vegetable pea cultivars, Shijiadacaiwan 1 (SJ1) and Chengwan 8 (CW8), respectively. Nine SJ1 and five CW8 M3 mutants showed resistant variations in Fusarium wilt, and the same five CW8 mutants in powdery mildew. These resistant variations were confirmed in M4 and M5 mutants as well. Then, we investigated the genetic variations and relationships of mutant lines using simple sequence repeat (SSR) markers. Among the nine effective SSR markers, the genetic diversity index and polymorphism information content (PIC) values were averaged at 0.55 and 0.46, which revealed considerable genetic variations in the mutants. The phylogenetic tree and population structure analyses divided the M3 mutants into two major groups at 0.62 genetic similarity (K = 2), which clearly separated the mutants of the two cultivars and indicated that a great genetic difference existed between the two mutant populations. Further, the two genetic groups were divided into five subgroups at 0.86 genetic similarity (K = 5) and each subgroup associated with resistant phenotypes of the mutants. Finally, the homologous PsMLO1 cDNA of five CW8 mutants that gained resistance to powdery mildew was amplified and cloned. A 129 bp fragment deletion was found in the PsMLO1 gene, which was in accord with er1-2. The findings provide important information on disease resistant and molecular variations of pea mutants, which is useful for pea production, new cultivar breeding, and the identification of resistance genes.
Accurate Determination of the Degree of Deacetylation of Chitosan Using UPLC–MS/MS
Authors: Xue Ting , Wang Wenqing , Yang Zhiyuan , Wang Fanjun , Yang Lei , Li Jian , Gan Hui , Gu Ruolan , Wu Zhuona , Dou Guifang , Meng Zhiyun
Published: August 2022

The mole fraction of deacetylated monomeric units in chitosan (CS) molecules is referred to as CS’s degree of deacetylation (DD). In this study, 35 characteristic ions of CS were detected using liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS/MS). The relative response intensity of 35 characteristic ion pairs using a single charge in nine CS samples with varying DDs was analyzed using 30 analytical methods. There was a good linear relationship between the relative response intensity of the characteristic ion pairs determined using ultrahigh performance (UP) LC–MS/MS and the DD of CS. The UPLC–MS/MS method for determining the DD of CS was unaffected by the sample concentration. The detection instrument has a wide range of application parameters with different voltages, high temperatures, and gas flow conditions. This study established a detection method for the DD of CS with high sensitivity, fast analysis, accuracy, stability, and durability.
Effect of Saccharides Coating on Antibacterial Potential and Drug Loading and Releasing Capability of Plasma Treated Polylactic Acid Films
Authors: Karakurt Ilkay , Ozaltin Kadir , Pištěková Hana , Vesela Daniela , Michael-Lindhard Jonas , Humpolícek Petr , Mozetič Miran , Lehocky Marian
Published: August 2022

More than half of the hospital-associated infections worldwide are related to the adhesion of bacteria cells to biomedical devices and implants. To prevent these infections, it is crucial to modify biomaterial surfaces to develop the antibacterial property. In this study, chitosan (CS) and chondroitin sulfate (ChS) were chosen as antibacterial coating materials on polylactic acid (PLA) surfaces. Plasma-treated PLA surfaces were coated with CS either direct coating method or the carbodiimide coupling method. As a next step for the combined saccharide coating, CS grafted samples were immersed in ChS solution, which resulted in the polyelectrolyte complex (PEC) formation. Also in this experiment, to test the drug loading and releasing efficiency of the thin film coatings, CS grafted samples were immersed into lomefloxacin-containing ChS solution. The successful modifications were confirmed by elemental composition analysis (XPS), surface topography images (SEM), and hydrophilicity change (contact angle measurements). The carbodiimide coupling resulted in higher CS grafting on the PLA surface. The coatings with the PEC formation between CS-ChS showed improved activity against the bacteria strains than the separate coatings. Moreover, these interactions increased the lomefloxacin amount adhered to the film coatings and extended the drug release profile. Finally, the zone of inhibition test confirmed that the CS-ChS coating showed a contact killing mechanism while drug-loaded films have a dual killing mechanism, which includes contact, and release killing.
Albumin Stimulates Epithelial Na+ Transport and Barrier Integrity by Activating the PI3K/AKT/SGK1 Pathway
Authors: Laube Mandy , Thome Ulrich H.
Published: August 2022

Albumin is a major serum protein and is frequently used as a cell culture supplement. It is crucially involved in the regulation of osmotic pressure and distribution of fluid between different compartments. Alveolar epithelial Na+ transport drives alveolar fluid clearance (AFC), enabling air breathing. Whether or not albumin affects AFC and Na+ transport is yet unknown. We therefore determined the acute and chronic effects of albumin on Na+ transport in fetal distal lung epithelial (FDLE) cells and the involved kinase pathways. Chronic BSA treatment strongly increased epithelial Na+ transport and barrier integrity in Ussing chambers. BSA did not elevate mRNA expression of Na+ transporters in FDLE cells after 24 h. Moreover, acute BSA treatment for 45 min mimicked the chronic effects. The elevated Na+ transport was caused by an increased maximal ENaC activity, while Na,K-ATPase activity remained unchanged. Acute and chronic BSA treatment lowered membrane permeability, confirming the increased barrier integrity observed in Ussing chambers. Western blots demonstrated an increased phosphorylation of AKT and SGK1, and PI3K inhibition abolished the stimulating effect of BSA. BSA therefore enhanced epithelial Na+ transport and barrier integrity by activating the PI3K/AKT/SGK1 pathway.
Incorporation of the New anti-Octadecaborane Laser Dyes into Thin Polymer Films: A Temperature-Dependent Photoluminescence and Infrared Spectroscopy Study
Authors: Capkova Tereza , Hanulikova Barbora , Sevcik Jakub , Urbanek Pavel , Antos Jan , Urbanek Michal , Kuritka Ivo
Published: August 2022

New anti-octadecaborane(22) laser dyes have been recently introduced. However, their application in solid thin films is limited, despite being very desirable for electronics. Spectroscopic methods, photoluminescence (PL), and infrared reflection–absorption spectroscopy (IRRAS), are here used to reveal structural responses to a temperature change in thin polymer films made of π- and σ-conjugated and non-conjugated polymers and anti-octadecaborane(22) and its tetra-alkylatedderivatives. It has been observed that borane clusters are not firmly fixed within polymer matrices and that their ability for diffusion out of the polymer film is unprecedented, especially at higher temperatures. This ability is related to thermodynamic transitions of polymer macromolecular chains. PL and IRRAS spectra have revealed a clear correlation with β-transition and α-transition of polymers. The influence of structure and molecular weight of a polymer and the concentration and the substitution type of clusters on mobility of borane clusters within the polymer matrix is demonstrated. A solution is proposed that led to an improvement of the temperature stability of films by 45 °C. The well-known spectroscopic methods have proved to be powerful tools for a non-routine description of the temperature behavior of both borane clusters and polymer matrices.
Rock Microhabitats Provide Suitable Thermal Conditions for Overwintering Insects: A Case Study of the Spongy Moth (Lymantria dispar L.) Population in the Altai Mountains
Authors: Ananko Grigory G. , Kolosov Aleksei V. , Martemyanov Vyacheslav V.
Published: August 2022

Many insect species overwinter in various rock shelters (cavities and crevices), but the microclimates of rock biotopes remain poorly understood. We investigated the temperature dynamics in rock microhabitats where clusters of egg masses of the wintering spongy moth Lymantria dispar L. (SM) were observed. Our research objective was to find the relation between the ovipositing behaviour of females and the landscape features in different parts of this species’ range. Studies of the ecology of the SM are important from a practical point of view, as the moth causes significant economic damage to forests of the Holarctic. We found that the average monthly temperature of rock surfaces in the studied microhabitats was 2–5 °C above the average air temperature. More importantly, the minimum temperatures in these microhabitats were 4–13 °C higher than the minimum air temperature. These results help to reassess the role of the mountain landscape in the spread of insect species. Rock biotopes provided a significant improvement in the conditions for wintering insects. We believe that, when modelling the spread of invasive species (such as the SM), it is necessary to account for the influence of rock biotopes that may facilitate shifts in the northern boundaries of their range.
Radiologic Evaluation of Uterine Vasculature of Uterus Transplant Living Donor Candidates: DUETS Classification
Authors: Kristek Jakub , Johannesson Liza , Clemons Matthew Paul , Kautznerova Dana , Chlupac Jaroslav , Fronek Jiri , Testa Giuliano , dePrisco Gregory
Published: August 2022

Uterus transplantation is a treatment solution for women suffering from absolute uterine factor infertility. As much as 19.5% of uterus-transplanted patients underwent urgent graft hysterectomy due to thrombosis/hypoperfusion. The necessity to identify candidates with high-quality uterine vasculature is paramount. We retrospectively evaluated and compared the imaging results with actual vascular findings from the back table. In this article, we present a novel radiologic grading scale (DUETS classification) for evaluating both uterine arteries and veins concerning their suitability for uterus procurement and transplantation. This classification defines several criteria for arteries (caliber, tapering, atherosclerosis, tortuosity, segment, take-off, and course) and veins (caliber, tapering, plethora, fenestrations, duplication/multiplicity, dominant route of venous drainage, radiologist’s confidence with imaging and assessment). In conclusion, magnetic resonance angiography can provide reliable information on uterine venous characteristics if performed consistently according to a well-established protocol and assessed by a dedicated radiologist. The caliber of uterine arteries seems to be inversely related to the time passed since the last delivery. We recommend that the radiologist comments on the reliability and confidence of the imaging study. It cannot be over-emphasized that the most crucial aspect of surgical imaging is the necessity of high-quality communication between a surgeon and a radiologist.
Association of Meibomian Gland Dysfunction with Oral Statin Use
Authors: Park Sun-Kyoung , Lee Ji-Hye , Hwang Ho-Sik , Kim Hyun-Seung , Han Kyung-Do , Na Kyung-Sun
Published: August 2022

This retrospective cross-sectional study aimed to determine the association of oral statin use, dry eye disease (DED), and meibomian gland dysfunction (MGD). A total of 93 subjects were included and divided into two groups: statin users (n = 45) and nonstatin users (n = 47). Significant differences were observed in the total cholesterol (p = 0.013), low-density lipoprotein (LDL) (p = 0.005), and meiboscore (p = 0.000) levels between the two groups. For stratified analysis, the statin group was divided into subgroups according to the type or dose of statin and total duration of statin use. However, there were no differences in clinical features between the subgroups. In multiple regression analysis, meiboscore was significantly associated with age (slope = 0.05, p = 0.00) and statin use (slope = −1.19, p = 0.00), with an R2 of 0.44. Thus, older adults and participants who do not use statin appeared to have higher scores. In conclusion, although the mechanism is unclear, statins may exert a protective effect on the meibomian gland. Further lipidomic studies are required to determine the pharmacological effects of statins on the meibomian gland and other meibum components.
Bleeding Patterns of Oral Contraceptives with a Cyclic Dosing Regimen: An Overview
Authors: Archer David F. , Mansour Diana , Foidart Jean-Michel
Published: August 2022

Bleeding irregularities are one of the major reasons for discontinuation of oral contraceptives (OCs), and therefore clinicians need to set expectations during consultations. In this review we provide an overview of bleeding data of recently marketed cyclic combined OCs (COCs) and one progestin-only pill (POP). We evaluated data from phase 3 trials (≥12 months) used to gain regulatory approval. Overall, each type of OC has its own specific bleeding pattern. These patterns however were assessed by using different bleeding definitions, which hampers comparisons between products. In COCs, the estrogen balances the effects of the progestin on the endometrium, resulting in a regular bleeding pattern. However, this balance seems lost if a too low dose of ethinylestradiol (EE) (e.g., 10 µg in EE/norethindrone acetate 1 mg) is used in an attempt to lower the risk of venous thromboembolism. Replacement of EE by 17β-estradiol (E2) or E2 valerate could lead to suboptimal bleeding profile due to destabilization of the endometrium. Replacement of EE with estetrol (E4) 15 mg in the combination with drospirenone (DRSP) 3 mg is associated with a predictable and regular scheduled bleeding profile, while the POP containing DRSP 4 mg in a 24/4 regimen is associated with a higher rate of unscheduled and absence of scheduled bleeding than combined products.
Predictive Markers of Crohn’s Disease in Small Bowel Capsule Endoscopy: A Retrospective Study of Small Bowel Capsule Endoscopy
Authors: Mattila Juho , Stenholm Teppo , Löyttyniemi Eliisa , Koffert Jukka
Published: August 2022

To distinguish between functional gastrointestinal disorders like irritable bowel syndrome (IBS) and mild small bowel Crohn′s disease (CD) can be a burden. The diagnosis of CD often requires small bowel capsule endoscopy (SBCE). The main goal of this research was to find predictive markers to rule out clinically significant small bowel CD without SBCE. A retrospective study of 374 patients who underwent SBCE for suspected small bowel CD in Turku University Hospital in 2012–2020 was conducted. We gathered the patient′s laboratory, imaging and endoscopic findings at the time of SBCE. SBCE findings were graded along CECDAI (Capsule Endoscopy Crohn’s Disease Activity Index)-scoring system. Fecal calprotectin (FC), serum albumin and ESR were significantly different with patients diagnosed with CD and those with not. Hb and CRP had no significant differences between the two groups. Sensitivity, specificity, PPV and NPV for FC < 50 ug/g were 96.4%, 19.6%, 34.6% and 92.5% and for CECDAI (cut-off value 3) 98.2%, 90.3%, 81.1% and 99.1%, respectively. A CECDAI-score of 3 would be a reasonable cut-off value for small bowel CD. Small bowel CD is possible with FC < 100 ug/g. Our results suggest a follow-up with FC before SBCE for patients with no endoscopic ileitis, negative imaging results and FC < 50 ug/g before SBCE.
Effect of Sinus Perforation with Flaplessly Placed Mini Dental Implants for Oral Rehabilitation: A 5-Year Clinical and Radiological Follow-Up
Authors: Van Doorne Luc , Hommez Geert , Bronkhorst Ewald , Meijer Gert , De Bruyn Hugo
Published: August 2022

Background: Flaplessly placed one-piece mini dental implants (MDI) are an option to support maxillary overdentures. Evenly distribution of the implants over the atrophic alveolar process implies a risk of accidental sinus perforation in the posterior area which could induce sinus-related pathology. Methods: Thirty-one patients received 5–6 maxillary MDIs. Schneiderian membrane swelling was assessed with CBCT at the deepest point of the sinus in the mid-sagittal plane prior to surgery (baseline), after 2 and 5 years. Additionally, subjective patient-reported rhinosinusitis complaints, the effect of smoking, and gender differences were investigated. Results: Mean thickness of the Schneiderian membrane was 2.87 mm at baseline, 3.15 mm at 2 years, and 4.30 mm at 5 years in 27 of 31 initially treated patients. MDI perforation was detected in 21/54 sinuses. At 2 years, perforation length does not affect membrane thickness whereas baseline swelling does. In smokers, each perforated mm induced 0.87 mm additional swelling. After 5 years, the effect of baseline swelling becomes smaller whereas perforation length became statistically significant, with 0.53 mm increase for every perforated mm. The effect of smoking lost its significance. No relations between gender, membrane thickness changes, or subjective clinical sinus complaints and MDI perforation were found. Conclusion: Accidental MDI sinus perforation induces Schneiderian membrane swelling but does not interfere with clinical sinusal outcome after 5 years.
Level of Physical Activity in Pregnant Populations from Different Geographic Regions: A Systematic Review
Authors: Silva-Jose Cristina , Sánchez-Polán Miguel , Barakat Rubén , Gil-Ares Javier , Refoyo Ignacio
Published: August 2022

The aim of this study was to examine the level of physical activity during pregnancy in different populations worldwide. An intensive search was carried out from February until May 2021. The inclusion criteria were original studies of healthy pregnant women, and the main study variable was the assessment of physical activity. A total of 110 out of 1451 studies were assessed for inclusion, using the Newcastle–Ottawa Scale for quality, and for the risk of bias. The 44 analyzed articles were divided into 5 tables according to the characteristics of the intervention and the validated instrument used to measure physical activity (PA). A total of 59.09% of the studies indicated that participants had a low level of physical activity during pregnancy. In addition, the median quality score of the studies was 7.12, and 77.27% of the studies were cataloged as having a high-quality score. Although international guidelines recommend that women without a contraindication engage in prenatal physical activity, the results of the present study show that the level of PA is too low for women to achieve scientifically proven maternal-fetal benefits. Failure to achieve the recommended levels of weekly physical activity could pose significant risks to maternal well-being.
A New Study on the Structure, and Phase Transition Temperature of Bulk Silicate Materials by Simulation Method of Molecular Dynamics
Authors: Nguyen Trong Dung , Long Van Cao , Ţălu Ştefan , Saraç Umut , Nguyen Dang Phu , Pham Huu Kien
Published: August 2022

In this paper, the structure and phase transition temperature of bulk silicate materials are studied by the simulation method (SM) of molecular dynamics (MD). In this research, all samples are prepared on the same nanoscale material model with the atomic number of 3000 atoms, for which the SM of MD is performed with Beest-Kramer-van Santen and van Santen pair interaction potentials under cyclic boundary conditions. The obtained results show that both the model size (l) and the total energy of the system (Etot) increase slowly in the low temperature (T) region (negative T values) at pressure (P), P = 0 GPa. However, the increase of l determines the Etot value with very large values in the high T region. It is found that l decreases greatly in the high T region with increasing P, and vice versa. In addition, when P increases, the decrease in the Etot value is small in the low T region, but large in the high T region. As a consequence, a change appears in the lengths of the Si-Si, Si-O, and O-O bonds, which are very large in the high T and high P regions, but insignificant in the low T and low P regions. Furthermore, the structural unit number of SiO7 appears at T > 2974 K in the high P region. The obtained results will serve as the basis for future experimental studies to exploit the stored energy used in semiconductor devices.
Improvement to Calculation Method of Flood Force on T-Girder Considering Entrapped Air in Chambers
Authors: Yang Wanli , Dou Yang , Qin Junwu , Yang Yuankang , Zhang Yuzhi , Zhang Weiyong
Published: August 2022

Entrapped air in the chambers of a T-girder bridge generates a considerably large vertical force which is harmful to the safety of the bridge superstructure. The contribution of the entrapped air is not considered when calculating flood force in the related specifications, and also it has not been taken into account by researchers. Two-dimensional, scaled-down models at a 1:40 scale are selected as the research object. Analytical and numerical methods are employed to investigate the contribution of entrapped air in T-girder chambers to flood forces. Results show that the entrapped air in the rear chambers could escape easier than that in the front chambers and the compressibility of entrapped air has a small influence on drag force and vertical force (generated by dynamic pressure) coefficients, but it reduces the buoyancy of the T-girder. Considering the entrapped air compressibility and entrapped air escape, the calculation method of the buoyancy of entrapped air is proposed to improve the precision of the existing flood force calculation method.
A Review of Progress and Applications of Automated Vacuum Mooring Systems
Authors: Yan Kaicheng , Zhang Shengdong , Oh Jungkeun , Seo Dae-Won
Published: August 2022

Compared with the traditional mooring system, the automated vacuum mooring system can meet the development needs of large-scale ship automation, port automation, and environmental protection. This review describes the latest research focuses, progress, applications, and future perspectives regarding the automated vacuum mooring system. First, the components, working principles, advantages, limits, and risks of the automated vacuum mooring system are discussed. Secondly, typical application cases of automated vacuum mooring systems are introduced, looking at two aspects of the ship-based system and shore-based system. Then, the routine maintenance of the automated vacuum mooring system is introduced. Finally, a discussion on the challenges and future perspectives of the automated vacuum mooring system is provided in this review. The advantages of an automated vacuum mooring system make it a potentially highly effective and economical option for a wider range of ship mooring than a traditional mooring system.
The Biosynthesis of 1-octene-3-ol by a Multifunctional Fatty Acid Dioxygenase and Hydroperoxide Lyase in Agaricus bisporus
Authors: Su Tongfu , Chen Yuannan , Liu Haohao , Gao Yuqian , Guo Jiawen , Li Yanan , Qi Yuancheng , Qiu Liyou
Published: August 2022

The biosynthetic pathway from linoleic acid to 1-octen-3-ol in Agaricus bisporus has long been established, in which linoleic acid is converted to 10-hydroperoxide (10-HPOD) by deoxygenation, and 10-HPOD is subsequently cleaved to yield 1-octene-3-ol and 10-oxodecanoic acid. However, the corresponding enzymes have not been identified and cloned. In the present study, four putative genes involved in oxylipid biosynthesis, including one lipoxygenase gene named AbLOX, two linoleate diol synthase genes named AbLDS1 and AbLDS2, and one hydroperoxide lyase gene named AbHPL were retrieved from the A. bisporus genome by a homology search and cloned and expressed prokaryotically. AbLOX, AbLDS1, and AbLDS2 all exhibited fatty acid dioxygenase activity, catalyzing the conversion of linoleic acid to generate hydroperoxide, and AbHPL showed a cleaving hydroperoxide activity, as was determined by the KI-starch method. AbLOX and AbHPL catalyzed linoleic acid to 1-octen-3-ol with an optimum temperature of 35 °C and an optimum pH of 7.2, whereas AbLDS1, AbLDS2, and AbHPL catalyzed linoleic acid without 1-octen-3-ol. Reduced AbLOX expression in antisense AbLOX transformants was correlated with a decrease in the yield of 1-octen-3-ol. AbLOX and AbHPL were highly homologous to the sesquiterpene synthase Cop4 of Coprinus cinerea and the yeast sterol C-22 desaturase, respectively. These results reveal that the enzymes for the oxidative cleavage of linoleic acid to synthesize 1-octen-3-ol in A. bisporus are the multifunctional fatty acid dioxygenase AbLOX and hydroperoxide lyase AbHPL.
Algorithm Aversion as an Obstacle in the Establishment of Robo Advisors
Authors: Filiz Ibrahim , Judek Jan René , Lorenz Marco , Spiwoks Markus
Published: August 2022

Within the framework of a laboratory experiment, we examine to what extent algorithm aversion acts as an obstacle in the establishment of robo advisors. The subjects had to complete diversification tasks. They could either do this themselves or they could delegate them to a robo advisor. The robo advisor evaluated all the relevant data and always made the decision which led to the highest expected value for the subjects’ payment. Although the high level of efficiency in the robo advisor was clear to see, the subjects only entrusted their decisions to the robo advisor in around 40% of cases. In this way, they reduced their success and their payment. Many subjects orientated themselves towards the 1/n-heuristic, which also contributed to their suboptimal decisions. As long as the subjects had to make decisions for others, they noticeably made a greater effort and were also more successful than when they made decisions for themselves. However, this did not have an effect on their acceptance of robo advisors. Even when they made decisions on behalf of others, the robo advisor was only consulted in around 40% of cases. This tendency towards algorithm aversion among subjects is an obstacle to the broader establishment of robo advisors.