bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell Differentiation of Bovine Milk Control Samples to Improve Prognosis of Mastitis Cure
Authors: Bunge Anne , Dreyer Sonja , Paduch Jan-Hendrik , Klocke Doris , Leimbach Stefanie , Wente Nicole , Nitz Julia , Krömker Volker
Published: February 2022

To optimise udder health at the herd level, identifying incurable mastitis cases as well as providing an adequate therapy and culling strategy are necessary. Cows with clinical mastitis should be administered antibiotic medication if it is most likely to improve mammary cure. The somatic cell count (SCC) in milk of the monthly implemented Dairy Herd Improvement (DHI) test represents the most important tool to decide whether a cow has a promising mammary cure rate. Differential cell count (DCC) facilitates the specification of the immunological ability of defence, for example by characterising leukocyte subpopulations or cell viability. The aim of this study was to assess the DCC and cell viability in DHI milk samples regarding the cytological (CC) and bacteriological cure (BC) of the udder within a longitudinal study, thereby gaining a predictive evaluation of whether a clinical mastitis benefits from an antibiotic treatment or not. The cows enrolled in this study had an SCC above 200,000 cells/mL in the previous DHI test. Study 1 assessed the CC by reference to the SCC of two consecutive DHI tests and included 1010 milk samples: 28.4% of the mammary glands were classified as cytologically cured and 71.6% as uncured. The final mixed logistic regression model identified the total number of non-vital cells as a significant factor associated with CC. An increasing amount of non-vital cells was related to a lower individual ability for CC. Cows which were in the first or second lactation possessed a higher probability of CC than cows having a lactation number above two. If animals developed a clinical mastitis after flow cytometric investigation, the BC was examined in study 2 by analysing quarter foremilk samples microbiologically. Taking 48 milk samples, 81.3% of the mammary glands were classified as bacteriologically cured and 18.7% as uncured. The percentage of total non-vital cells tended to be lower for cows which were cured, but no significance could be observed. This study revealed that the investigation of the proportion of non-vital cells in DHI milk samples can enhance the prognosis of whether an antibiotic treatment of clinical mastitis might be promising or not. Prospectively, this tool may be integrated in the DHI tests to facilitate the decision between therapy or culling.
Similarities in Virulence and Extended Spectrum Beta-Lactamase Gene Profiles among Cefotaxime-Resistant Escherichia coli Wastewater and Clinical Isolates
Authors: Liedhegner Elizabeth , Bojar Brandon , Beattie Rachelle E. , Cahak Caitlin , Hristova Krassimira R. , Skwor Troy
Published: February 2022

The World Health Organization has identified antibiotic resistance as one of the largest threats to human health and food security. In this study, we compared antibiotic resistance patterns between ESBL-producing Escherichia coli from human clinical diseases and cefotaxime-resistant environmental strains, as well as their potential to be pathogenic. Antibiotic susceptibility was tested amongst clinical isolates (n = 11), hospital wastewater (n = 22), and urban wastewater (n = 36, both influent and treated effluents). Multi-drug resistance predominated (>70%) among hospitalwastewater and urban wastewater influent isolates. Interestingly, isolates from clinical and urban treated effluents showed similar multi-drug resistance rates (~50%). Most hospital wastewater isolates were Phylogroup A, while clinical isolates were predominately B2, with a more diverse phylogroup population in urban wastewater. ESBL characterization of cefotaxime-resistant populations identified blaCTX-M-1 subgroup as the most common, whereby blaKPC was more associated with ceftazidime and ertapenem resistance. Whole-genome sequencing of a carbapenemase-producing hospital wastewater E. coli strain revealed plasmid-mediated blaKPC-2. Among cefotaxime-resistant populations, over 60% of clinical and 30% of treated effluent E. coli encoded three or more virulence genes exhibiting a pathogenic potential. Together, the similarity among treated effluent E. coli populations and clinical strains suggest effluents could serve as a reservoir for future multi-drug resistant E. coli clinical infections.
Benzothiazole Derivatives as Multifunctional Antioxidant Agents for Skin Damage: Structure–Activity Relationship of a Scaffold Bearing a Five-Membered Ring System
Authors: Djuidje Ernestine Nicaise , Barbari Riccardo , Baldisserotto Anna , Durini Elisa , Sciabica Sabrina , Balzarini Jan , Liekens Sandra , Vertuani Silvia , Manfredini Stefano
Published: February 2022

Skin diseases often give multifactorial damages; therefore, the development of multifunctional compounds represents a suitable approach especially against disorders that are induced by oxidative stress. Thus, taking into account the successful results we achieved on benzimidazoles, we have devised a new series of isosteric benzothiazoles and investigated their antioxidant, photoprotective, antifungal and antiproliferative activity. Particular attention has been paid to synergistic antioxidant and photoprotective properties. For compounds 9a and 10a, a multifunctional profile was outlined, supported by an excellent filtering capacity, mainly UVB, which has higher capacities than those of the reference PBSA which is currently in the market as a UV sunscreen filter. The two compounds were also the best in terms of growth inhibition of dermatophytes and Candida albicans, and 10a also showed good antioxidant activity. Furthermore, 9a was also effective on melanoma tumor cells (SK-Mel 5), making these compounds good candidates in the development of new skin protective and preventive agents.
Real-Scale Experimental Evaluation of Energy and Thermal Regulation Effects of PCM-Based Mortars in Lightweight Constructions
Authors: Andrés Manuel , Rebelo Filipe , Corredera Álvaro , Figueiredo António , Hernández José L. , Ferreira Víctor M. , Bujedo Luis A. , Vicente Romeu , Morentin Francisco , Samaniego Jesús
Published: February 2022

Lightweight construction is experiencing a significant market implementation with sustained growth both for new buildings and retrofitting purposes. Despite the acknowledged advantages of this type of construction, their reduced thermal inertia can jeopardize indoor thermal comfort levels while leading to higher energy consumption due to high indoor temperature fluctuations and overheating rates. The incorporation of phase change materials (PCMs) into constructive solutions for lightweight buildings is a promising strategy to guarantee adequate thermal comfort conditions. Particularly, the utilization of mortars embedding PCMs as an indoor wall coating for new and existing buildings represents a solution that has not been widely explored in the past and needs further development and validation efforts. This work pursues the analysis of the thermal regulation effects generated by two thermally-enhanced mortars incorporating microencapsulated PCMs with different operating temperature ranges. To that end, an experimental campaign was conducted in Valladolid (Spain) to address the investigation of the proposed solution under a real-scale relevant environment. The proposed mortars were applied as an indoor coating to the envelope of a single-zone lightweight construction that was monitored (under different weather conditions along 1-year monitoring campaign) together with an identical building unit where the mortar was not added to the constructive base layer. The analysis of indoor temperature fluctuations under free-floating operating mode as well as the energy consumption of HVAC equipment under controlled-temperature operation was specifically targeted. Results derived from the continuous monitoring campaign revealed lower temperature fluctuations during summer and shoulder seasons, reducing indoor temperature peaks by 1–2 °C, and producing a time delay of 1–1.5 h into the temperature wave. A clear reduction in energy use due to the incorporation of the PCM-based indoor coating panels is also observed. Thus, this experimental research contributes to proving that the use of innovative mortars incorporating embedded PCMs enables the development of high-end efficient building solutions with innovative materials towards a sustainable built environment.
Development of Passive Fire Protection Mortars
Authors: Caetano Hugo , Laím Luís , Santiago Aldina , Durães Luísa , Shahbazian Ashkan
Published: February 2022

During a fire event, the stability of steel structures may be compromised, and structural collapse may occur due to the loss of their mechanical resistance as the temperature increases. One of the solutions to reduce this problem is the protection with a coating using enhanced fire-resistant mortars. This paper reports a detailed experimental work aiming to develop gypsum and cement-based mortars for passive fire protection and evaluate their composition’s effect in the final thermal performance. Two types of specimens were tested: (i) small specimens composed of a mortar coating (10 mm thick) and one steel plate and (ii) square section short tubular steel columns with 20 mm of coating. The evaluation of the thermal protection was carried out by (a) measuring the thermal gradient between the exposed surface of the protected steel plate under high temperatures and the mortar-steel interface and (b) assessing the fire resistance of the short steel columns. It was concluded that the compositions with gypsum binder present better thermal insulation than the cementitious compositions. Additionally, the introduction of nano- and microparticles of silica still slightly improved the thermal insulation of the tested compositions.
Over and beyond the Primate baubellum Surface: A “Jewel Bone” Shielded in Museums
Authors: Spani Federica , Morigi Maria Pia , Bettuzzi Matteo , Scalici Massimiliano , Carosi Monica
Published: February 2022

Computed Tomography (CT), mostly used in the medical field, has also recently been involved in Cultural Heritage studies, thanks to its efficiency and total non-invasiveness. Due to the large variety of sizes and compositions typical of Cultural Heritage objects, different X-ray sources, detectors, and setups are necessary to meet the different needs of various case studies. Here, we focus on the use of micro-CT to explore the morphology and shape of a small, neglected bone found inside the clitoris of non-human primates (the baubellum), which we obtained by accessing two prestigious primatological collections of the American Museum of Natural History (New York, NY, USA) and the National Museum of Natural History (Washington, DC, USA). Overcoming methodological limits imposed by the absence of homologous landmarks, we combined the use of the non-invasive 3D micro-CT and a recently released landmark-free shape analysis (the alpha-shape technique) to objectively describe and quantify the shape complexity of scanned primate baubella. Micro-CT provided high-resolution results, overcoming constraints linked to museum policy about non-disruptive sampling and preserving samples for future research. Finally, it proved appropriate as post-mortem sampling had no impact on protected wild primate populations.
Optical Studies in Red/NIR Persistent Luminescent Cr-Doped Zinc Gallogermanate (ZGGO:Cr)
Authors: Batista Maria S. , Rodrigues Joana , Relvas Maria S. , Zanoni Júlia , Girão Ana V. , Pimentel Ana , Costa Florinda M. , Pereira Sónia O. , Monteiro Teresa
Published: February 2022

Zn1+xGa2−2xGexO4 (ZGGO:Cr)-persistent phosphor, with a molar fraction, x, of x = 0.1, doped with a 0.5% molar of chromium, was synthesised via solid-state reaction at 1350 °C for 36 h. X-ray diffraction measurements and Raman spectroscopy evidence a single crystalline phase corresponding to the cubic spinel structure. Room temperature (RT) photoluminescence (PL) and afterglow decay profiles were investigated using above and below bandgap excitation. In both cases, persistent PL was observed for almost 8 h, mainly originating from a Cr3+ defect, the so-called N2 optical centre. RT PL excitation and diffuse reflectance allow identification of the best pathways of Cr3+ red/NIR emission, as well as estimation of the ZGGO bandgap energy at 4.82 eV. An in-depth investigation of the observed luminescence at 15 K and temperature-dependent PL under site-selective excitation reveals the spectral complexity of the presence of several optically active Cr3+ centres in the ZGGO host that emit in almost the same spectral region. Furthermore, the temperature dependence of the R-lines’ intensity indicates the existence of thermal populating processes between the different optical centres. Such observations well account for a wide distribution of defect trap levels available for carrier capture/release, as measured by the persistent luminescence decay, from which the carriers are released preferentially to the N2 Cr3+-related optical centre.
Atomization of Microfibrillated Cellulose and Its Incorporation into Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Reactive Extrusion
Authors: Freitas Pedro A. V. , Barrrasa Hector , Vargas Fátima , Rivera Daniel , Vargas Maria , Torres-Giner Sergio
Published: February 2022

The present study focuses on the preparation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films that were reinforced with cellulose microstructures to obtain new green composite materials for sustainable food packaging applications. The atomization of suspensions of microfibrillated cellulose (MFC) successfully allowed the formation of ultrathin cellulose structures of nearly 3 µm that were, thereafter, melt-mixed at 2.5, 5, and 10 wt % with PHBV and subsequently processed into films by thermo-compression. The most optimal results were attained for the intermediate MFC content of 5 wt %, however, the cellulose microstructures showed a low interfacial adhesion with the biopolyester matrix. Thus, two reactive compatibilizers were explored in order to improve the properties of the green composites, namely the multi-functional epoxy-based styrene-acrylic oligomer (ESAO) and the combination of triglycidyl isocyanurate (TGIC) with dicumyl peroxide (DCP). The chemical, optical, morphological, thermal, mechanical, and barrier properties against water and aroma vapors and oxygen were analyzed in order to determine the potential application of these green composite films in food packaging. The results showed that the incorporation of MFC yielded contact transparent films, whereas the reactive extrusion with TGIC and DCP led to green composites with enhanced thermal stability, mechanical strength and ductility, and barrier performance to aroma vapor and oxygen. In particular, this compatibilized green composite film was thermally stable up to ~280 °C, whereas it showed an elastic modulus (E) of above 3 GPa and a deformation at break (ɛb) of 1.4%. Moreover, compared with neat PHBV, its barrier performance to limonene vapor and oxygen was nearly improved by nine and two times, respectively.
Analysis of the Acceleration Response Spectra of Single-Layer Spherical Reticulated Shell Structures
Authors: Fan Feng , Zhi Xudong , Li Wenliang
Published: February 2022

In recent years, earthquake disasters have seriously damaged nonstructural components, so it is necessary to study their seismic performance. However, the existing scholarly research mainly concentrates on multistorey and high-rise buildings, and there are still deficiencies in the analysis of the seismic performance of the nonstructural components in large-span structures under seismic action. In this paper, the acceleration responses of a single-layer spherical reticulated shell structure are compared with those described in the current seismic design codes of the nonstructural components, and it is found that the current codes are not fully applicable to the seismic design of the nonstructural components in reticulated shell structures. The calculation formulas of the acceleration response spectra of single-layer spherical shell nodes are theoretically derived, and the shell node acceleration response spectra are affected by higher-order modes, orthogonal horizontal seismic input directions, and the membrane stiffness of the shell nodes. The variations in the acceleration responses of the shell nodes with node position and rise-to-span ratio are analysed, and a design method for the equivalent seismic action of the nonstructural components in a single-layer spherical reticulated shell with a roofing system is proposed.
Applying Deep Learning Models to Analyze Users’ Aspects, Sentiment, and Semantic Features for Product Recommendation
Authors: Lai Chin-Hui , Tseng Kuo-Chiuan
Published: February 2022

As there is a huge amount of information on the Internet, people have difficulty in sorting through it to find the required information; thus, the information overload problem becomes a significant issue for users and online businesses. To resolve this problem, many researchers and applications have proposed recommender systems, which apply user-based collaborative filtering, meaning it only considers the users’ rating history to analyze their preferences. However, users’ text data may contain users’ preferences or sentiment information, and such information can be used to analyze users’ preferences more precisely. This work proposes a method called the aspect-based deep learning rating prediction method (ADLRP), which can extract the aspects, sentiment, and semantic features from users’ and items’ reviews. Then, the deep learning method is used to generate users’ and items’ latent factors. According to these three features, the matrix factorization method is applied to make rating predictions for items. The experimental results show that the proposed method performs better than the traditional rating prediction methods and conventional artificial neural networks. The proposed method can precisely and efficiently extract the sentiments and semantics of each aspect from review texts and enhance the prediction performance of rating predictions.
Fabrication of an Anti-Icing Aluminum Alloy Surface by Combining Wet Etching and Laser Machining
Authors: Xia Annan , He Lei , Qie Shihang , Zhang Jingchen , Li Hanlong , He Ning , Hao Xiuqing
Published: February 2022

The phenomenon of icing on the surface of the fuselage while aircraft pass through clouds has an impact on flight safety. This aircraft icing may adversely affect metrological conditions and cause aerodynamic mechanical effects, resulting in a threat to flight safety. This research aims to fabricate an anti-icing surface on a 2524 aluminum alloy material by combining laser machining and wet etching. The microstructure surfaces were obtained by laser, the nanostructured surfaces were obtained by wet etching, and the hierarchical structures were prepared through a combination of these two processes. The contact angle, icing delay performance, icing adhesion, and dynamic water repellency of three kinds of textured surfaces and smooth surface were tested and compared comprehensively through experiments. The experimental findings have shown that the hierarchical surface has the best superhydrophobic properties, and the freezing time of droplets on the hierarchical surface can be extended 10 times. In addition, the ice adhesion on the surface of the hierarchical structure decreased by approximately 75% compared to the untreated samples. The surface of the hierarchical structure showed the best dynamic water repellency. The proposed hybrid laser machining–wet etching fabricating method has the potential to avoid aircraft icing.
Colonization of Lactobacillus rhamnosus GG in Cirrhinus molitorella (Mud Carp) Fingerling: Evidence for Improving Disease Resistance and Growth Performance
Authors: Yu Yang-Mei , Poon Peggy Miu-Yee , Sharma Aayushi Ashok , Chan Sidney Man-Ngai , Lee Fred Wang-Fat , Mo Ian Wing-Yin , Sze Eric Tung-Po
Published: February 2022

The use of probiotic bacteria can not only enhance the nutritional utilization of fish feeds to produce more biomass but can also provide a practically “safer” alternative to the fish farming industry to reduce the abuse of antibiotics and drugs. This study investigated the possibility of colonizing Lactobacillus rhamnosus strain GG (LGG) to the intestine of Cirrhinus molitorella (mud carp) fingerling. Colonization of LGG was observed in gut tissue after 14 days of administration with a diet supplemented with 1 × 108 CFU/mL LGG. Moreover, growth performance parameters of the LGG-supplemented diet group, including relative weight gain, feed conversion ratio and feed efficiency, were found about two-fold higher than the control group after 60 days. In addition, fish fed with an LGG-supplemented diet for 60 days showed substantial resistance against the infection of pathogenic bacterial Aeromonas hydrophila, with a relative survival rate of up to 57% compared to the control group. In summary, the results indicated that LGG as dietary supplement for mud carp fingerling can enhance nutrition utilization and better protect fish against the infection of Aeromonas hydrophila. The results provide an insight to the fish farming industry, encouraging a reduction in the use of antibiotics and drugs and the production of “safer” mud carp for the market at a manageable cost.
Taiwan Stock Tape Reading Periodically Using Web Scraping Technology with GUI
Authors: Lin Chun-Feng , Yang Sheng-Chih
Published: February 2022

Stock tape reading involves surveilling stock prices once in a while and recording stock prices. The method of observing stock prices may be television or stock exchange. The time step for recoding stock prices is every stock user’s experience and their theory, perhaps 3 min or 2 h and so on. As an example, the Taiwan stock market starts at 9:00 a.m. to 13:30 p.m. It will have a 4 h operating time. Splitting the 4 h operating time for tape reading is the skill of stock users. The stock price sequence generated by tape reading can be predicted by stock users, but finally, it is the stock user’s experience. Therefore, the meaning of tape reading is to record the stock price, but its concept should have no prediction purpose. This study used thread technology and proposed a tape-reading method with web scraping. This method can periodically scrape stock prices and generate a stock price sequence to Excel file. This application can satisfy the demand of these stock users, who are called day trading users. Because these day trading users want to gain stock price sequences minute by minute, rather than the stock exchange format day by day, and also ones which are better than the those provided by the stock website service, because its stock sequence format is limited and not normalized, these day trading users think that minute-by-minute stock price sequences are very clear to forecast. This study implemented the prior scheme and designed the GUI to query a company’s stock price and its stock news, even per second, etc., and how long it took to update the stock price, and the GUI also included a time-up feature to stop scraping stock prices if users just wanted to scrape stock prices periodically.
Potential Influence of the Atlantic Multidecadal Oscillation in the Recent Climate of a Small Basin in Central Mexico
Authors: Montero-Martínez Martín José , Pita-Díaz Oscar , Andrade-Velázquez Mercedes
Published: February 2022

One of the main current challenges is detecting changes in the climate at the regional level. The present study tried to address this issue by looking for some influence of large-scale climate oscillations on the climate of a small and complex topography basin in Central Mexico. We collected temperature and precipitation data from 44 climate stations within an area of up to 20 km around the Apatlaco River sub-basin (~30 km south of Mexico City) during the period 1950–2013. Posteriorly, quality analysis and homogenization of the climate databases were performed by using the Climatol algorithm. We analyzed the trend of five ETCCDI climate indices through several statistical tests. Finally, we calculated simple Pearson correlations of those indices with four climate oscillation indices that have affected Mexico’s climate in the recent past. The results revealed that the Atlantic Multidecadal Oscillation had a clear influence on four of the five indices analyzed in the study area. The summer days and the extreme maximum and minimum temperatures accounted for a small increase in the temperature of the middle east (urban) basin compared to the middle west (rural), which could be a manifestation of the heat island effect or the difference in soil type (and therefore albedo) of the two zones. As expected, the midsummer drought effect predominated in most of the sub-basin, with only the uppermost part showing monsoon-type precipitation during a typical year.
SARS-CoV-2 RNA Recovery from Air Sampled on Quartz Fiber Filters: A Matter of Sample Preservation?
Authors: Licen Sabina , Zupin Luisa , Martello Lorenzo , Torboli Valentina , Semeraro Sabrina , Gardossi Anna Lilian , Greco Enrico , Fontana Francesco , Crovella Sergio , Ruscio Maurizio , Palmisani Jolanda , Di Gilio Alessia , Piscitelli Prisco , Pallavicini Alberto , Barbieri Pierluigi
Published: February 2022

The airborne route of transmission of SARS-CoV-2 was confirmed by the World Health Organization in April 2021. There is an urge to establish standardized protocols for assessing the concentration of SARS-CoV-2 RNA in air samples to support risk assessment, especially in indoor environments. Debates on the airborne transmission route of SARS-CoV-2 have been complicated because, among the studies testing the presence of the virus in the air, the percentage of positive samples has often been very low. In the present study, we report preliminary results on a study for the evaluation of parameters that can influence SARS-CoV-2 RNA recovery from quartz fiber filters spotted either by standard single-stranded SARS-CoV-2 RNA or by inactivated SARS-CoV-2 virions. The analytes were spiked on filters and underwent an active or passive sampling; then, they were preserved at −80 °C for different numbers of days (0 to 54) before extraction and analysis. We found a mean recovery of 2.43%, except for the sample not preserved (0 days) that showed a recovery of 13.51%. We found a relationship between the number of days and the recovery percentage. The results presented show a possible issue that relates to the quartz matrix and SARS-CoV-2 RNA recovery. The results are in accordance with the already published studies that described similar methods for SARS-CoV-2 RNA field sampling and that reported non-detectable concentrations of RNA. These outcomes could be false negatives due to sample preservation conditions. Thus, until further investigation, we suggest, as possible alternatives, to keep the filters: (i) in a sealed container for preservation at 4 °C; and (ii) in a viral transport medium for preservation at a temperature below 0 °C.
Transboundary Air Pollution Transport of PM10 and Benzo[a]pyrene in the Czech–Polish Border Region
Authors: Volná Vladimíra , Hladký Daniel , Seibert Radim , Krejčí Blanka
Published: February 2022

The article is occupied with the evaluation of the transboundary transport of pollutants in the Czech–Polish border region (between the Moravian-Silesian region and the Silesian Voivodeship) in Central Europe. It focuses on the evaluation of concentrations of benzo[a]pyrene (BaP) and suspended particles of PM10 depending on meteorological conditions, especially wind direction. The whole area of interest is heavily affected with air pollution of BaP and PM10. Limits of BaP and PM10 are still exceeded. Annual concentrations of benzo[a]pyrene are even several times higher than the value of its annual limit. The elaboration follows the results of the Czech–Polish project “Air Silesia”, which dealt with air pollution and the transboundary transport of pollutants in this area and took place in 2010 to 2013. Within this project, a higher transport of pollutants from Poland to the Czech Republic was established. The evaluation of the dependences of PM10 concentrations is based on hourly and daily data of PM10 and hourly data of meteorological quantities. To assess the dependences of daily BaP and PM10 concentrations, a methodology for evaluating daily types of wind direction was implemented into the processing. The results confirm that the problem of above-limit concentrations of BaP and PM10 in the Moravian-Silesian Region in the Czech Republic and the Silesian Voivodeship in Poland remains. The article confirms there is a higher transport of PM10 concentrations from Poland to the Czech Republic in the area of interest. Higher transport in the same direction is also predicted for daily concentrations of benzo[a]pyrene, although this cannot be clearly confirmed due to the lack of more detailed and identifiable data.
Reliability-Based Design Optimization of Structures Considering Uncertainties of Earthquakes Based on Efficient Gaussian Process Regression Metamodeling
Authors: Xiao Yanjie , Yue Feng , Wang Xinwei , Zhang Xun’an
Published: February 2022

The complexity of earthquakes and the nonlinearity of structures tend to increase the calculation cost of reliability-based design optimization (RBDO). To reduce computational burden and to effectively consider the uncertainties of ground motions and structural parameters, an efficient RBDO method for structures under stochastic earthquakes based on adaptive Gaussian process regression (GPR) metamodeling is proposed in this study. In this method, the uncertainties of ground motions are described by the record-to-record variation and the randomness of intensity measure (IM). A GPR model is constructed to obtain the approximations of the engineering demand parameter (EDP), and an active learning (AL) strategy is presented to adaptively update the design of experiments (DoE) of this metamodel. Based on the reliability of design variables calculated by Monte Carlo simulation (MCS), an optimal solution can be obtained by an efficient global optimization (EGO) algorithm. To validate the effectiveness and efficiency of the developed method, it is applied to the optimization problems of a steel frame and a reinforced concrete frame and compared with the existing methods. The results show that this method can provide accurate reliability information for seismic design and can deal with the problems of minimizing costs under the probabilistic constraint and problems of improving the seismic reliability under limited costs.
Deep-Dwelling Populations of Mediterranean Corallium rubrum and Eunicella cavolini: Distribution, Demography, and Co-Occurrence
Authors: Carugati Laura , Moccia Davide , Bramanti Lorenzo , Cannas Rita , Follesa Maria Cristina , Salvadori Susanna , Cau Alessandro
Published: February 2022

Corallium rubrum and Eunicella cavolini are two octocorals, reported as co-occurring species in the deep rocky habitats of the Mediterranean Sea with a high hydrodynamic and moderate eutrophication. Their spatial distribution and demography in the deep sea are mainly affected by temperature and direct and indirect anthropogenic activities; however, knowledge of the factors that potentially influence their co-existence is scarce. This paper provides novel data on the distribution and demography of these two species, at depths between 50 and 290 m in the Western Mediterranean Sea, providing insights on their co-occurrence. Both species exhibited the highest population density at deeper sites (>150 m), showing an inverse size–density relation. Density values ranged from 0.03 colonies m−2 to 32 and 80 col. m−2 for yellow gorgonian and red coral, respectively. The two species co-occurred in 13% of the total frames examined, mostly dwelling between 120 and 160 m depth. Distance-based linear modeling (DistLM) emphasized that when co-occurring the variability of the two species’ densities were significantly driven by the density—rather than the morphology (i.e., height)—of the other species. We stress the need for further studies to elucidate the possible mutual effects of suspension feeders and to test the role of different environmental factors potentially influencing inter-specific relationships.
Degradation of CP4-EPSPS with a Psychrophilic Bacterium Stenotrophomonas maltophilia 780
Authors: Peng Yanhong , He Wencong , Li Yunjing , Liu Lu , Deng Binyang , Yan Guangbo , Yang Jun , Wang Fei , Ma Lixin , Wu Gang , Zhai Chao
Published: February 2022

CP4-EPSPS (Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase) protein showed remarkable thermostability and was highly resistant to proteases, such as trypsin. In order to eliminate the pollution of CP4-EPSPS from the accumulated straws to the surrounding environment during the winter, the present study investigated the extracellular proteases of 21 psychrophilic strains isolated from the south polar region. The results indicated that Stenotrophomonas maltophilia 780 was able to degrade CP4-EPSPS at 18 °C efficiently. Further study indicated that it was able to grow in the extract of Roundup Ready soybean at 18 °C, with CP4-EPSPS degraded to an undetectable level within 72 h. The extracellular proteases of Stenotrophomonas maltophilia 780 are thermo-sensitive, with an optimal temperature of 65 °C. The genomic sequencing result indicated that this strain had more than a hundred putative protease and peptidase coding genes, which may explain its high capability in decomposing CP4-EPSPS.
HMG Proteins from Molecules to Disease
Authors: Barreiro-Alonso Aída , Vizoso-Vázquez Ángel , Lamas-Maceiras Mónica , Cerdán María-Esperanza
Published: February 2022

High Mobility Group (HMG) proteins are today the focus of interest due to their participation in human degenerative diseases and inflammatory responses [...]
Targeting the Endocannabinoidome in Pancreatic Cancer
Authors: Falasca Valerio , Falasca Marco
Published: February 2022

Pancreatic Ductal adenocarcinoma (PDAC), the most common malignancy of the pancreas, is an aggressive and lethal form of cancer with a very high mortality rate. High heterogeneity, asymptomatic initial stages and a lack of specific diagnostic markers result in an end-stage diagnosis when the tumour has locally advanced or metastasised. PDAC is resistant to most of the available chemotherapy and radiation therapy treatments, making surgery the most potent curative treatment. The desmoplastic tumour microenvironment contributes to determining PDAC pathophysiology, immune response and therapeutic efficacy. The existing therapeutic approaches such as FDA-approved chemotherapeutics, gemcitabine, abraxane and folfirinox, prolong survival marginally and are accompanied by adverse effects. Several studies suggest the role of cannabinoids as anti-cancer agents. Cannabinoid receptors are known to be expressed in pancreatic cells, with a higher expression reported in pancreatic cancer patients. Therefore, pharmacological targeting of the endocannabinoid system might offer therapeutic benefits in pancreatic cancer. In addition, emerging data suggest that cannabinoids in combination with chemotherapy can increase survival in transgenic pancreatic cancer murine models. This review provides an overview of the regulation of the expanded endocannabinoid system, or endocannabinoidome, in PDAC and will explore the potential of targeting this system for novel anticancer approaches.
The Halophyte Dehydrin Sequence Landscape
Authors: Ghanmi Siwar , Graether Steffen P. , Hanin Moez
Published: February 2022

Dehydrins (DHNs) belong to the LEA (late embryogenesis abundant) family group II, that comprise four conserved motifs (the Y-, S-, F-, and K-segments) and are known to play a multifunctional role in plant stress tolerance. Based on the presence and order of these segments, dehydrins are divided into six subclasses: YnSKn, FnSKn, YnKn, SKn, Kn, and KnS. DHNs are rarely studied in halophytes, and their contribution to the mechanisms developed by these plants to survive in extreme conditions remains unknown. In this work, we carried out multiple genomic analyses of the conservation of halophytic DHN sequences to discover new segments, and examine their architectures, while comparing them with their orthologs in glycophytic plants. We performed an in silico analysis on 86 DHN sequences from 10 halophytic genomes. The phylogenetic tree showed that there are different distributions of the architectures among the different species, and that FSKn is the only architecture present in every plant studied. It was found that K-, F-, Y-, and S-segments are highly conserved in halophytes and glycophytes with a few modifications, mainly involving charged amino acids. Finally, expression data collected for three halophytic species (Puccinillia tenuiflora, Eutrema salsugenium, and Hordeum marinum) revealed that many DHNs are upregulated by salt stress, and the intensity of this upregulation depends on the DHN architecture.
Maternal Metabolic State and Fetal Sex and Genotype Modulate Methylation of the Serotonin Receptor Type 2A Gene (HTR2A) in the Human Placenta
Authors: Horvatiček Marina , Perić Maja , Bečeheli Ivona , Klasić Marija , Žutić Maja , Kesić Maja , Desoye Gernot , Nakić Radoš Sandra , Ivanišević Marina , Hranilovic Dubravka , Štefulj Jasminka
Published: February 2022

The serotonin receptor 2A gene (HTR2A) is a strong candidate for the fetal programming of future behavior and metabolism. Maternal obesity and gestational diabetes mellitus (GDM) have been associated with an increased risk of metabolic and psychological problems in offspring. We tested the hypothesis that maternal metabolic status affects methylation of HTR2A in the placenta. The prospective study included 199 pairs of mothers and healthy full-term newborns. Genomic DNA was extracted from feto-placental samples and analyzed for genotypes of two polymorphisms (rs6311, rs6306) and methylation of four cytosine residues (−1665, −1439, −1421, −1224) in the HTR2A promoter region. Placental HTR2A promoter methylation was higher in male than female placentas and depended on both rs6311 and rs6306 genotypes. A higher maternal pre-gestational body mass index (pBMI) and, to a lesser extent, diagnosis of GDM were associated with reduced HTR2A promoter methylation in female but not male placentas. Higher pBMI was associated with reduced methylation both directly and indirectly through increased GDM incidence. Tobacco use during pregnancy was associated with reduced HTR2A promoter methylation in male but not female placentas. The obtained results suggest that HTR2A is a sexually dimorphic epigenetic target of intrauterine exposures. The findings may contribute to a better understanding of the early developmental origins of neurobehavioral and metabolic disorders associated with altered HTR2A function.
Proteasome-Mediated Regulation of GATA2 Expression and Androgen Receptor Transcription in Benign Prostate Epithelial Cells
Authors: Azeem Waqas , Olsen Jan Roger , Hellem Margrete Reime , Hua Yaping , Marvyin Kristo , Ke Xisong , Øyan Anne Margrete , Kalland Karl-Henning
Published: February 2022

GATA2 has been shown to be an important transcription factor together with androgen receptor (AR) in prostate cancer cells. Less is known about GATA2 in benign prostate epithelial cells. We have investigated if GATA2 exogenous expression in prostate epithelial basal-like cells could induce AR transcription or luminal differentiation. Prostate epithelial basal-like (transit amplifying) cells were transduced with lentiviral vector expressing GATA2. Luminal differentiation markers were assessed by RT-qPCR, Western blot and global gene expression microarrays. We utilized our previously established AR and androgen-dependent fluorescence reporter assay to investigate AR activity at the single-cell level. Exogenous GATA2 protein was rapidly and proteasome-dependently degraded. GATA2 protein expression was rescued by the proteasome inhibitor MG132 and partly by mutating the target site of the E3 ligase FBXW7. Moreover, MG132-mediated proteasome inhibition induced AR mRNA and additional luminal marker gene transcription in the prostate transit amplifying cells. Different types of intrinsic mechanisms restricted GATA2 expression in the transit amplifying cells. The appearance of AR mRNA and additional luminal marker gene expression changes following proteasome inhibition suggests control of essential cofactor(s) of AR mRNA expression and luminal differentiation at this proteolytic level.
Critical Care in SARS-CoV-2 Infected Pregnant Women: A Prospective Multicenter Study
Authors: Álvarez Bartolomé Ana , Abdallah Kassab Nadia Akram , Cruz Melguizo Sara , de la Cruz Conty María Luisa , Forcen Acebal Laura , Abascal Saiz Alejandra , Pintado Recarte Pilar , Martinez Varea Alicia , Cerrillos Gonzalez Lucas , García Fernández Javier , Martínez Pérez Oscar
Published: February 2022

Evidence suggests that pregnant women are at a higher risk of complications compared to the general population when infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the reasons that lead them to need intensive care are not clear. This is a prospective multicenter study of SARS-CoV-2 positive pregnant women, registered by the Spanish Obstetric Emergency Group, with the objective to define the characteristics of the mothers who were admitted to the Intensive Care Unit (ICU) and to investigate the causes and risk factors for ICU admission. A total of 1347 infected pregnant women were registered and analyzed, of whom, 35 (2.6%) were admitted to the ICU. No differences in maternal characteristics or comorbidities were observed between ICU and non-ICU patients, except for in vitro fertilization and multiple pregnancies. The main causes of admission to the ICU were non-obstetric causes (worsening of the maternal condition and respiratory failure due to SARS-CoV-2 pneumonia, 40%) and a combination of coronavirus disease 2019 (COVID-19) symptoms and obstetrical complications (31.4%). The multivariable logistic analysis confirmed a higher risk of ICU admission when pre-eclampsia or hemorrhagic events coexist with pneumonia. The incidence of thromboembolic events and disseminated intravascular coagulation were also significantly higher among patients admitted to the ICU. Therefore, surveillance and rapid intervention should be intensified in SARS-CoV-2 infected pregnant women with the mentioned risk factors and complications. Emphasis should always be placed on anticoagulant therapy in these patients due to the increased thromboembolic risk, C-section surgery and immobilization in the ICU.
Transient Elastography in Community Alcohol Services: Can It Detect Significant Liver Disease and Impact Drinking Behaviour?
Authors: Subhani Mohsan , Harman David J. , Scott Robert A. , Bennett Lucy , Wilkes Emilie A. , James Martin W. , Aithal Guruprasad P. , Ryder Stephen D. , Guha Indra Neil
Published: February 2022

Introduction: Alcohol is the leading cause of cirrhosis in Western populations. The early identification of high-risk drinkers followed by intervention is an effective way to reduce harm. We aim to assess the feasibility of integrating transient elastography (TE) into community alcohol services, and to determine its impact on modifying drinking behaviours. Method: A prospective cohort study was conducted at a community alcohol clinic in Nottingham, UK (April 2012 to March 2014). Patients (>18 years) with a primary alcohol problem were recruited. Those known to liver services or those known to have chronic liver disease were excluded. Significant liver fibrosis was defined by a liver stiffness of >8 kilopascal (kPa). Follow-up was for a minimum of six months. Data were descriptively analysed for significant differences between patients with a normal liver stiffness versus raised liver stiffness. Results: 156 patients were invited; n = 87 attended and n = 86 underwent successful TE. The majority were male (n = 53, 70.0%), and the mean age was 46.3 years (SD ± 9.8). Median liver stiffness was 6.9 kPa (range 3.1–75.0kPa). Clinically significant liver fibrosis was identified in n = 33 (38.4%), of which n = 6 were in the cirrhotic range (≥15 kPa). The baseline median self-reported alcohol intake for normal stiffness was 126 units per week (range 24–378) and in raised stiffness was 149.0 units per week (range 39.0–420.0); this difference was nonsignificant (p = 0.338). The median reduction in self-reported alcohol intake in the whole cohort was 65.0 units per week (range 27.0–88.0, p < 0.001); in the normal liver stiffness group it was 25.0 units per week (range 18.0–75.0, p = 0.154), and in the raised liver stiffness group it was 78.5 units per week (range 36.0–126.0, p < 0.001). Conclusion: The study demonstrated that transient elastography is a feasible tool to stratify clinically significant liver disease in community alcohol services. It can stimulate a change in high-risk drinking behaviour and a normal liver stiffness result does not provide false reassurance to participants.
Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma
Authors: Iqneibi Shahad , Nazzal Jamil , Owda Basma , Sultan Hala , Amoudi Runa , Amarin Justin Z. , Al-Ghnimat Sura , Ahram Mamoun , Al-Hussaini Maysa
Published: February 2022

p27 and p57 are tumor suppressors that are dysregulated in many cancers. We investigated the immunohistochemical expression of p27 and p57 in ependymoma, with a secondary emphasis on cyclin D1, nestin, and Ki-67. Sixty-five patients diagnosed with ependymoma were included. Clinical and tumoral data were retrieved, and the expression of p27, p57, cyclin D1, nestin, and Ki-67 was measured. Pearson’s χ2 test was used to measure associations and the Kaplan–Meier method was used for survival analysis. p27 underexpression was significantly associated with pseudopalisading necrosis in tumors with foci of necrosis (p = 0.004). Cyclin D1 overexpression was associated with intracranial (p = 0.044), recurrent (p = 0.022) and grade 3 tumors (p = 0.016); nestin overexpression was associated with supratentorial (p = 0.025), mitotically active (p < 0.001), and grade 3 tumors (p = 0.004); Ki-67 overexpression was associated with supratentorial (p = 0.044) and grade 3 tumors (p < 0.001) and the 3 main features of anaplasia. None of the markers were intercorrelated or predictive of overall survival. In conclusion, p27 underexpression in tumors with foci of necrosis signals a pseudopalisading pattern. Cyclin D1, nestin, and Ki-67 are useful markers in ependymoma, but evidence-based cutoff values are required to standardize this interpretation.
The Relationship between Wind Pressure and Pressure Coefficients for the Definition of Wind Loads on Buildings
Authors: Picozzi Vincenzo , Malasomma Antonio , Avossa Alberto Maria , Ricciardelli Francesco
Published: February 2022

Wind induced pressures on buildings are the product of a velocity pressure and a pressure coefficient. The way in which these two quantities are calculated has changed over the years, and Design Codes have been modified accordingly. This paper tracks the evolution of the approach to wind loading of buildings from the practice in the 1950s, mainly referring to the Swiss Code SIA, to the most recent advances including probabilistic methods, internet databases, and advanced modelling of meteorological phenomena.