bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on the Impact of 3D Printing and Artificial Intelligence on Education and Learning Process
Authors: Chun Hyunjin
Published: November 2021

Artificial intelligence is part of our future and has allowed the creation of truly advanced devices. Now 3D printing technology can also make full use of AI, and with the development of this technology, it is expected to be used in various fields in the era of the Fourth Industrial Revolution. This technology can be used not only in manufacturing but also in various fields such as architecture and medical care and is recognized as an essential technology for future generations. Educating the students about the use and impact of 3D technology is even more important than it was ever; however, not enough research has been conducted on specific teaching methods for 3D printing technology education. This paper, therefore, studies the future education method using 3D printing technology for children. The research method of this study is a qualitative research method in which interviews and participant observation methods are used. This qualitative research method was developed by James P. Spradley, an American cultural anthropologist. The findings of this study are as follows. Children can make products easily by using 3D programs and can develop creativity through this product design process. Based on these results, this paper aims to present the curriculum and teaching methods of 3D printing that can be practically utilized in public education courses. This education will be a preparatory step for the micro-manufacturing industry in the future. The findings of this study are helpful in developing the courses of future education.
Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High‐Fat Diet‐Induced Obesity
Authors: Kim Chang Yeon , Ahn Jang Ho , Han Do Hyun , NamKoong Cherl , Choi Hyung Jin
Published: November 2021

The hypothalamus plays a central role in the integrated regulation of feeding and energy homeostasis. The hypothalamic arcuate nucleus (ARC) contains a population of neurons that express orexigenic and anorexigenic factors and is thought to control feeding behavior via several neuronal circuits. In this study, a comparative proteomic analysis of low‐fat control diet‐ (LFD‐) and high‐fat diet‐ (HFD‐) induced hypothalamic ARC was performed to identify differentially expressed proteins (DEPs) related to changes in body weight. In the ARC in the hypothalamus, 6621 proteins (FDR < 0.01) were detected, and 178 proteins were categorized as DEPs (89 upregulated and 89 downregulated in the HFD group). Among the Gene Ontology molecular function terms associated with the DEPs, protein binding was the most significant. Fibroblast growth factor receptor substrate 2 (Frs2) and SHC adaptor protein 3 (Shc3) were related to protein binding and involved in the neurotrophin signaling pathway according to Kyoto Encyclopedia of Genes and Genomes analysis. Furthermore, high‐precision quantitative proteomic analysis revealed that the protein profile of the ARC in mice with HFD‐induced obesity differed from that in LFD mice, thereby offering insight into the molecular basis of feeding regulation and suggesting Frs2 and Shc3 as novel treatment targets for central anorexigenic signal induction.
Buckling and Vibration Performance of a Composite Laminated Plate with Elastic Boundaries Subjected to Local Thermal Loading
Authors: Sun Yonggan
Published: November 2021

In this paper, a model is established for the calculation of the vibrations of a composite laminated plate with elastic boundary conditions subjected to local thermal loading. The model is based on first‐order shear deformation theory using the finite element method. The influence of boundary conditions, heating area, and heating location on buckling and vibrations of a composite laminated plate was investigated, and there were two stages in which the critical temperature increased sharply during the transition from free boundary to simply supported and rigid fixed boundaries. The thermal buckling of locally heated laminated plates is generally not checked in practical applications unless the heated area exceeds approximately 10% of the total area of the plates. The stronger the boundary constraint is, the greater the influence of the heated area is on the vibrational frequencies of the composite laminated plate.
Tracheobronchoplasty and Diaphragmatic Plication under VV ECMO for Combined ECAC and Diaphragmatic Paralysis
Authors: Tatari Mehmet M. , Abia-Trujillo David , Thomas Mathew , Patel Neal M. , Fernandez-Bussy Sebastian , Hazelett Britney N. , Johnson Margaret M.
Published: November 2021

The coexistence of expiratory central airway collapse and diaphragmatic paralysis presents a diagnostic and treatment challenge. Both entities are underrecognized causes of dyspnea, cough, sputum production, and orthopnea. Optimal treatment must be individualized and is best achieved by a multidisciplinary team. We present a case of a patient with profound functional impairment from dyspnea and hypoxemia due to expiratory central airway collapse, complicated by bronchiectasis from recurrent respiratory infections, and diaphragmatic paralysis.
Quality of Cattle Meat and Its Compositional Constituents
Authors: Geletu Umer Seid , Usmael Munera Ahmednur , Mummed Yesihak Yusuf , Ibrahim Abdulmuen Mohammed
Published: November 2021

Meat is the most valuable livestock product since it is one of the main sources of protein for human consumption. Meat quality can be evaluated according to the following parameters: pH, amount of lactic acid, volatile fatty acids, bounded water, solubility of proteins, color, and tenderness. The meat composition and physical properties of muscles have been characterized for ensuring improved eating quality. Thus, the purpose of this paper was to review the major chemical compositional and physicochemical properties of meat and, at the same time, its quality attributes and factors that affect quality of meat. A number of structural features of meat as connective tissue, muscle fibers, and tendon that attaches the muscle to the bone are visible in joint meat examined through naked eyes. Water is quantitatively the most important component of meat comprising up to 75% of weight. Meat is also composed of amino acids, fatty acids, vitamins, minerals, and other important ingredients. Quality factors perceived by consumers are related to sensory attributes (e.g., color, tenderness, and flavor), nutritional properties (e.g., calories, vitamins’ content, and fatty acids’ profile), and appearance (e.g., exudation, marbling, and visible amount of fat). However, fresh meat quality can be defined instrumentally including composition, nutrients, color, water-holding capacity, tenderness, functionality, flavors, spoilage, and contamination. Visual inspection based on sensory quality attributes and different chemical methods are used to analyze meat quality. Other methods such as computer vision and imaging spectroscopy, gas chromatographic analysis, near-infrared technology, dual-energy X-ray absorptiometry, and computerized tomography scanning are also used in the meat industry. So, the aim of the present review is to review quality characteristics of cattle meat and its composition constituents.
Optimizing Kenmi Manipulation Courses of High School Sports Based on CDIO Model under the Background of Cloud Computing
Authors: Liu Luosha
Published: November 2021

Cloud computing is an increase, use, and delivery model of Internet-based services. It usually involves the provision of dynamic transactions and often virtualized resources through the Internet and provides the most reliable and secure data storage center. Therefore, cloud computing technology has features of cost saving and scalability. In view of this, the design and research of an autonomous learning platform based on the CDIO concept can make full use of cloud computing technology. The CDIO teaching model is a combination of theory and practice, closely linking the cultivation of knowledge, abilities, and personal qualities, subverting the previous education methods. Although it is an engineering field, it is based on an open system. Penetrating into all fields of education, the teaching methods of aerobics in colleges and universities are as practical as the CDIO teaching model and must be applied to practice. The independent learning ability, critical thinking, and team cohesion emphasized by the CDIO teaching model are also involved in the teaching of aerobics. CDIO is a key issue that needs to be solved urgently in talent training. CDIO is just a platform and framework. Under this framework, different schools and majors can integrate their own teaching systems and teaching content as needed. The reform of aerobics courses is guided by the educational framework and philosophy of CDIO and designed in accordance with the standards of CDIO. It is very important to plan the system and training of this course. This paper researches and analyzes the application of CDIO teaching mode in college sports aerobics under the background of cloud computing.
Solid Pseudopapillary Neoplasm of the Pancreas: Unfolding an Intriguing Condition
Authors: Cruz Manuel António Alves , Moutinho-Ribeiro Pedro , Costa-Moreira Pedro , Macedo Guilherme
Published: November 2021

Pancreatic cancer is one of the most lethal malignant neoplasms, with a 1-year survival rate after diagnosis of 24%, and a 5-year survival rate of only 9%. While this illustrates the behavior of its main histologic type – ductal adenocarcinoma, there are other histologic subtypes of pancreatic cancer that can harbor excellent prognosis. Solid pseudopapillary neoplasm, described as a rare low-grade malignant neoplasm by the World Health Organization, is the best example of that, having an overall 5-year survival rate of about 97%. Not only the prognosis, but everything about this entity is unique: its histogenesis, epidemiology, presentation, imaging characteristics, cytology features, immunohistochemical profile, and treatment. This explains the urge to improve our understanding about this entity and thus our ability to accurately recognize and manage it. Having this in mind, this article aims to summarize the most relevant topics regarding this entity.
Vascular Smooth Muscle Cells in Aortic Aneurysm: From Genetics to Mechanisms
Authors: Lu Haocheng , Du Wa , Ren Lu , Hamblin Milton H. , Becker Richard C. , Chen Y. Eugene , Fan Yanbo
Published: November 2021

Aortic aneurysm, including thoracic aortic aneurysm and abdominal aortic aneurysm, is the second most prevalent aortic disease following atherosclerosis, representing the ninth‐leading cause of death globally. Open surgery and endovascular procedures are the major treatments for aortic aneurysm. Typically, thoracic aortic aneurysm has a more robust genetic background than abdominal aortic aneurysm. Abdominal aortic aneurysm shares many features with thoracic aortic aneurysm, including loss of vascular smooth muscle cells (VSMCs), extracellular matrix degradation and inflammation. Although there are limitations to perfectly recapitulating all features of human aortic aneurysm, experimental models provide valuable tools to understand the molecular mechanisms and test novel therapies before human clinical trials. Among the cell types involved in aortic aneurysm development, VSMC dysfunction correlates with loss of aortic wall structural integrity. Here, we discuss the role of VSMCs in aortic aneurysm development. The loss of VSMCs, VSMC phenotypic switching, secretion of inflammatory cytokines, increased matrix metalloproteinase activity, elevated reactive oxygen species, defective autophagy, and increased senescence contribute to aortic aneurysm development. Further studies on aortic aneurysm pathogenesis and elucidation of the underlying signaling pathways are necessary to identify more novel targets for treating this prevalent and clinical impactful disease.
Subacute AMD3100 Treatment Is Not Efficient in Neonatal Hypoxic-Ischemic Rats
Authors: Spiess Daiane Aparecida , Campos Raquel Maria Pereira , Conde Luciana , Didwischus Nadine , Boltze Johannes , Mendez-Otero Rosalia , Pimentel-Coelho Pedro Moreno
Published: November 2021

Background and Purpose:

Despite the advances in treating neonatal hypoxic-ischemic encephalopathy (HIE) with induced hypothermia, the rates of severe disability are still high among survivors. Preclinical studies have indicated that cell therapies with hematopoietic stem/progenitor cells could improve neurological outcomes in HIE. In this study, we investigated whether the administration of AMD3100, a CXCR4 antagonist that mobilizes hematopoietic stem/progenitor cells into the circulation, has therapeutic effects in HIE.

Methods:

P10 Wistar rats of both sexes were subjected to right common carotid artery occlusion or sham procedure, and then were exposed to hypoxia for 120 minutes. Two subcutaneous injections of AMD3100 or vehicle were given on the third and fourth day after HIE. We first assessed the interindividual variability in brain atrophy after experimental HIE and vehicle treatment in a small cohort of rats. Based on this exploratory analysis, we designed and conducted an experiment to test the efficacy of AMD3100. Brain atrophy on day 21 after HIE was defined as the primary end point. Secondary efficacy end points were cognitive (T-water maze) and motor function (rotarod) on days 17 and 18 after HIE, respectively.

Results:

AMD3100 did not decrease the brain atrophy in animals of either sex. Cognitive impairments were not observed in the T-water maze, but male hypoxic-ischemic animals exhibited motor coordination deficits on the rotarod, which were not improved by AMD3100. A separate analysis combining data from animals of both sexes also revealed no evidence of the effectiveness of AMD3100 treatment.

Conclusions:

These results indicate that the subacute treatment with AMD3100 does not improve structural and functional outcomes in a rat HIE model.
Vorapaxar as an Alternative for Ticagrelor Resistance in Neuroendovascular Intervention
Authors: Mamaril‐Davis James C. , Aguilar‐Salinas Pedro , Brasiliense Leonardo B. , Cosgrove Richard , Dawod Judy , Dumont Travis M. , El‐Ghanem Mohammad
Published: November 2021

Background

Perioperative dual‐antiplatelet therapy for flow diversion limits thromboembolic complications. However, resistance to dual‐antiplatelet therapy medications remains a concern for neuroendovascular intervention. To date, there is no standardized approach for resistance to ADP receptor antagonists.

Methods

We report a case of ticagrelor resistance for flow diversion of an intracranial aneurysm treated with vorapaxar, as well as a narrative review of the literature for previous cases of ticagrelor resistance.

Results

Flow diversion with the Pipeline embolization device was deployed for a left internal carotid artery blister aneurysm and bilateral internal carotid artery dissecting pseudoaneurysms. The patient had 3 thromboembolic complications while on dual‐antiplatelet therapy with ticagrelor or prasugrel, leading to transition of antiplatelet therapy to vorapaxar. At 84 days follow‐up, the patient was fully recovered with complete occlusion of the aneurysms.

Conclusion

Our case suggests that vorapaxar is a promising alternative for patients with ticagrelor resistance in flow diversion–treated intracranial aneurysms. High‐quality randomized controlled trials are needed to elucidate the safety and efficacy of vorapaxar in neuroendovascular procedures.
Data and Model Operations in Computational Sciences: The Examples of Computational Embryology and Epidemiology
Authors: Li Vigni Fabrizio
Published: November 2021

Computer models and simulations have become, since the 1960s, an essential instrument for scientific inquiry and political decision making in several fields, from climate to life and social sciences. Philosophical reflection has mainly focused on the ontological status of the computational modeling, on its epistemological validity and on the research practices it entails. But in computational sciences, the work on models and simulations are only two steps of a longer and richer process where operations on data are as important as, and even more time and energy-consuming than modeling itself. Drawing on two study cases—computational embryology and computational epidemiology—this article contributes to filling the gap by focusing on the operations of producing and re-using data in computational sciences. The different phases of the scientific and artisanal work of modelers include data collection, aggregation, homogenization, assemblage, analysis and visualization. The article deconstructs the ideas that data are self-evident informational aggregates and that data-driven approaches are exempted from theoretical work. More importantly, the paper stresses the fact that data are constructed and theory laden not only in their fabrication, but also in their reusing.
History as Engagement: The Historical Epistemology of Raymond Aron
Authors: Simons Massimiliano
Published: November 2021

Raymond Aron was a student of Léon Brunschvicg, a representative of French historical epistemology. This article explores Aron’s relation to this tradition through three claims. First of all, it contests that Raymond Aron’s philosophy of history constituted a complete break with this tradition. Secondly, resituating Aron in this tradition is valuable, because it highlights how Aron’s own philosophy of history is to be understood as a normative project, seen as an alternative to that of Brunschvicg. Finally, Aron’s philosophy can still hold valuable lessons for present-day historical epistemology and history and philosophy of science in general.
Indonesian Biology Teachers’ Perceptions about Socio-Scientific Issue-Based Biology Instruction
Authors: Subiantoro Agung W. , Treagust David , Tang Kok-Sing
Published: November 2021

Promoting socio-scientific issue (SSI)-based instruction in Indonesian science classrooms requires competent science teachers. To understand teachers’ perceptions about the implementation of SSI-based instruction, a case study involving four biology teachers engaged in a teacher professional development program was conducted. The program consisted of four phases: reflection on teachers’ prior teaching experience and background knowledge, 3-day SSI-based teaching workshop, collaborative development and implementation of SSI-based learning in biology, and post-implementation reflections by teachers. Teachers’ perceptions were gathered via interviews and written reflections, which were analyzed qualitatively with an explanation building mode approach. Findings indicated a positive development along four dimensions: knowledge about SSIs and scientific literacy, the necessity of including SSIs in science instruction, situational factors related to addressing SSIs in class, and teachers’ attitude towards teaching SSIs. Further research needs to be conducted in Indonesian contexts to be able to extend the SSI teacher professional development program to different regions.
Changes in Parliaments - Parliaments in Change
Authors: Borbély Boglárka
Published: November 2021

Hungary’s University of Public Service and Brill Publishers (Leiden, Holland) staged a joint online event on 3 May 2021 about the reactions of Parliaments as traditional institutions to the fast-changing political, social and economic environment of our age. The conference was occasioned by the launch of the International Journal of Parliamentary Studies. Timed to coincide with the Day of Parliament, the scientific event also commemorated the inaugural session of Hungary’s first freely elected Parliament on 2 May 1990. The conference was moderated by Zsolt Szabó, the new journal’s chief editor, as well as associate professor at the University of Public Service and the Károli Gáspár University of the Reformed Church.
An annotated checklist of chewing lice (Phthiraptera: Amblycera, Ischnocera) from Slovakia
Authors: OŠLEJŠKOVÁ LUCIE , KRIŠTOFÍK JÁN , TRNKA ALFRÉD , SYCHRA OLDŘICH
Published: November 2021

This checklist includes taxa of chewing lice from published records, old collections, and recently collected material from birds and mammals in Slovakia. Data from established collections correspond to five different periods: (1) 1925–1939, collection of Karel Pfleger; (2) 1946–1978, collection of František Balát; (3) 1974–1985, collection of Vladimír Straka; (4) 1997–2012, collection of Ján Krištofík; and (5) 2008–2019, a collection made by the authors of this paper. A total of 255 species of feather lice—67 amblyceran species in 22 genera of families Laemobothriidae, Menoponidae and Ricinidae, and 188 ischnoceran species in 54 genera of the family Philopteridae—and 366 host-louse associations are listed from 171 bird species in 21 orders. In addition, eight species of chewing lice in five genera of the family Trichodectidae are listed from eight species of mammals. Species of chewing lice are reported from about 240 different locations throughout the territory of Slovakia. Also, 43 species of lice and 20 host-louse associations for Slovakia, as well as four host-louse associations for the world, are included as new records. A host-louse list of recorded species is also given.
Heat Waves and Innovation in Air Conditioning in the United States
Authors: Noy Ilan , Strobl Eric
Published: November 2021

This study investigates whether extreme heat episodes (heat waves) have contributed to the development of air conditioning (AC) technology in the United States. To this end, we use weather data to identify days at which heat and relative humidity were above levels comfortable to the human body, and match these with patent data at the county level for nearly a hundred years. We find that in the 2 years after a county has experienced extreme heat AC patents increase. Overall, average extreme heat exposure results in an increase of 7.5% greater innovation. We find no similar increase in the frequency of non-AC-related patent filings, and therefore conclude that heat waves result in innovation targeting their mitigation.

Significance Statement

The possibility of more extreme heat because of global warming has raised the question of whether society will be able to invent new technology to adapt to the likely greater frequency and severity of heat waves. The purpose of this paper is to consider the development of air conditioning in the United States and investigate whether extreme heat has indeed driven innovation in cooling technology. It is shown that, in counties with episodes of extreme heat, the number of air conditioning patents filed increased in the aftermath of these episodes, but that this increase was short-lived.
TRUMP, BOLSONARO, AND THE FRAMING OF THE COVID-19 CRISIS
Authors: Béland Daniel , Rocco Philip , Segatto Catarina Ianni , Waddan Alex
Published: November 2021

In the aftermath of the global COVID-19 crisis, whereas many world leaders enacted swift lockdown orders and robust testing regimes to preserve public health and to speed up economic recovery, Donald Trump in the United States and Jair Bolsonaro in Brazil responded to outbreaks by publicly downplaying the significance of the crisis and argued that overly restrictive health measures would create too sizable an economic risk. These two presidents have done much to weaken democracy and trust in government. In this article, we examine the extent to which two institutions in each country––federalism and the party system––impacted the ways in which they framed the COVID-19 crisis and policy responses to it in 2020, especially during the first months of the pandemic. Our evidence suggests that each of these institutions provided opportunities for both leaders to reconstruct public understandings of the crisis while deflecting blame for negative public-health outcomes.
Are You a Werewolf? Teaching Symbolic Interaction Theory through Game Play
Authors: Conner Christopher T. , Baxter Nicholas M.
Published: November 2021

In this article, we report on the implementation of using the game Werewolf as a student-centered applied-learning activity to teach symbolic interaction theory and concepts. Engaging with symbolic interaction theory can be a powerful experience for students due to its potential to challenge taken-for-granted assumptions and analyze students’ everyday life experiences. However, some students may have difficulty grasping the specific details and overall significance underlying the perspective. Moreover, research has shown that undergraduate students often have significant levels of anxiety when confronted with sociological theory in both introductory and upper division theory courses. We aim to address recommendations to incorporate more active learning approaches to social theory by outlining an applied-learning activity based on the role-playing game Werewolf. In the article, we review Werewolf and provide a step-by-step guide on how to implement the activity in the classroom and summarize findings from student assessments and classroom evaluations.
Progressive enlargement of thoracic ossification of the ligamentum flavum in professional baseball pitchers: a report of two cases
Authors: Kato Kinshi , Otoshi Kenichi , Hakozaki Michiyuki , Konno Shin-ichi
Published: November 2021

Both systemic and local factors might influence the development of ossification of the ligamentum flavum (OLF); however, whether specific physical activities play a role in the development of OLF remains unclear. This report presents two cases of thoracic radiculopathy owing to progressive enlargement of thoracic OLF within a few years in Japanese professional baseball pitchers. Two left-handed, Japanese, professional baseball pitchers in their 20s experienced thoracic radiating pain. Mild hypesthesia of the lower thoracic vertebral levels was observed, and an increase in the size of the OLF within a few years was identified in the lower thoracic spine using computed tomography and magnetic resonance imaging. Neither patient exhibited myelopathy and were, therefore, treated conservatively. Both patients were able to return to playing in top condition within 4 months after onset. The findings in these cases suggest that thoracic OLF might increase in size and become exacerbated in certain individuals, such as professional pitchers, who experience repeated, localized, mechanical stress on the thoracic spine, irrespective of age.
Celebrating the chameleon educational policy reforms of 2050
Authors: Clutterbuck Jennifer
Published: November 2021

The Chameleon Educational Policy Reforms’ (CEPR) 25th anniversary was celebrated at the Global General Assembly with the 2075 Decennial Analysis of Schooling (DecAS) announcement of the attainment of a benefit-cost ratio of >1.0. The attainment of a global positive net value of education is directly linked to the educational reforms established to ‘provide the right access to the right education for all people’ (CEPR, 2050). The Chameleon reforms, informed by The Algorithm, produce policies that instantly adapt to the learning environment and needs of students. Barriers that effect students’ learning are removed, in stark contrast to historic processes that viewed students as the barrier to be removed from learning environments. The case studies presented in this paper are guided by three questions: ‘Where are we now?’, ‘How did we get here?’, and ‘Are we there yet?’. Questioning the ‘here and now’, directs a look back from educational engagement currently governed by the CEPR to key moments and movements in the attainment of past grand policy announcements that no child be left behind to live in poverty. And ‘yet’, leads to critical consideration of the ongoing engagement with The Algorithm. Artifacts detailing the 2045 commencement of annual donations from the richest two percent to fully fund global education remain sealed. The results of the annual donations are, however, publicly available; and some would say exploited (Gerve, 2072). Achieving self-sustainable economic cost-benefit status for the CEPR may release the Donators from those agreements and untether society from the Donators.
Barley microgreen incorporation in diet-controlled diabetes and counteracted aflatoxicosis in rats
Authors: Mohamed Sara M , Abdel-Rahim Emam A , Aly Tahany AA , Naguib AbdelMoneim M , Khattab Marwa S
Published: November 2021

Increased environmental pollution and unhealthy lifestyle are blamed for escalated chronic diseases. Exposure to aflatoxins was recently suggested to have a role in the increased incidence of type 2 diabetes mellitus. Diet modification and consumption of different functional food are now gaining attention, especially in diabetes management. This study investigates the effect of a diet containing barley microgreen against diabetes induced by streptozotocin with or without aflatoxin administration in rats. Barley microgreen was rich in 3′-Benzyloxy-5,6,7,4′-tetramethoxyflavone (48.8% of total) followed by 5β,7βH,10α-Eudesm-11-en-1α-ol (18.46%). Streptozotocin injection and/or aflatoxin administration significantly elevated glucose level, decreased insulin level, decreased β-cell function, deteriorated liver and kidney function parameters, and induced oxidative stress in the liver. Histopathology revealed irregular small-sized islets and decreased area % of insulin-positive beta cells in the pancreas, hepatic degeneration, nephropathy, and neuropathy in diabetic and/or aflatoxin administered rats compared to control. Barley microgreen diet fed to diabetic rats with or without aflatoxin alleviated all evaluated parameters. Barley microgreen diet also ameliorated the toxic effect of aflatoxin. In conclusion, exposure to aflatoxin aggravated diabetes and its complication. The incorporation of barley microgreen in the diet was able to control type 2 diabetes mellitus and the improved outcomes observed with barley microgreen treatments involved or occurred in conjunction with improved biomarkers of oxidative stress.
Anatomic Measurement and Variability Analysis of the Anterior Talofibular Ligament and Calcaneofibular Ligament of the Ankle
Authors: Yang Han , Su Minghao , Chen Zhimin , Qu Rongmei , Yuan Zhirong , Yuan Jiajie , He Shanli , Li Zeyu , Liu Chang , Xiao Zhaoming , Liang Haibin , Ouyang Jun , Dai Jingxing
Published: November 2021

Background:

The anterior talofibular ligament (ATFL) and calcaneofibular ligament (CFL) contribute greatly to the overall stability of the ankle joint; however, ATFL and combined ATFL-CFL sprains are common. Anatomic reconstruction of the lateral collateral ligament with grafts has been proposed for patients with poor tissue quality or inadequate local tissue. Anatomic reconstruction of the lateral ankle ligaments requires a good understanding of their anatomic location.

Purpose:

To describe the anatomy of the ATFL and CFL ligaments quantitatively and qualitatively and explore the relationship of some morphological parameters.

Study Design:

Descriptive laboratory study.

Methods:

A total of 66 adult ankle specimens were analyzed for ATFL band type, origin, length, width, thickness, and angle between the ATFL and CFL, and 73 adult ankle specimens were used for measuring the origin of the CFL. The coefficient of variation was used to describe and compare the respective variability of angle, length, width, and thickness. The origin of the ATFL was labeled as point A, and the leading edge of the CFL intersection with the articular surface of the calcaneus was considered point B.

Results:

The ATFL had a variable number of bands. A high degree of variability (coefficient of variation >0.2) was seen for most morphological measurements of the ATFL. In addition, the length of distance AB also varied. The CFL originated at the tip of the fibula in only 9% of specimens. It was found more commonly at the anterior border of the lateral malleolus (4.94 ± 1.70 mm from the tip). The angle between the ATFL and CFL was consistent at 100° to 105º.

Conclusion:

A fair amount of variability of ATFL length, width, and thickness were found in our study, with less variability in the ATFL-CFL angle. Most CFLs attached anterior to the tip of the fibula.

Clinical Relevance:

Providing relevant anatomic data of ATFL and CFL is important in ensuring proper surgical treatment of ankle joint injuries.
Substance use and associated factors among preparatory school students in Kolfe-Keranyo sub-city of Addis Ababa, Ethiopia
Authors: Seid Leila , Gintamo Binyam , Mekuria Zelalem Negash , Hassen Hussien Seid , Gizaw Zemichael
Published: November 2021

Background

Substance abuse is a worldwide problem that primarily affects adolescents, resulting in chronic health complications as well as psychosocial challenges and economic losses. However, the magnitude of the problem and the factors that contribute to it are not well studied in Ethiopia, particularly in the study area. As a result, this study was carried out to determine the prevalence and associated factors of substance use among preparatory school students in the Kolfe-Keraniyo sub-city of Addis Ababa, Ethiopia.

Methodology

An institution-based cross-sectional study of 383 randomly selected preparatory school students in the Kolfe-Keraniyo sub-city was conducted. The data were gathered using a pretested self-administered structured questionnaire. Multivariable binary logistic regression analysis was employed to identify factors associated with substance use based on the adjusted odds ratio (AOR) and 95% confidence interval (CI) with p values less than 0.05.

Result

This study revealed that the lifetime prevalence of substance use among preparatory students in Kolfe-Keraniyo sub-city, Addis Ababa, Ethiopia, was 26.5% (95% CI, 22.2, 30.7%). Specifically, 16% drunk alcohol, 9.6% smoked cigarette, and 9.4% chewed khat. The 16.3% were current users, of which 8.3% were drinkers, 6.4% were smokers, and 5.9% were khat chewers. Substance use was significantly associated with being male (AOR, 3.3; 95% CI, 1.284, 8.613), having alcohol drinking family member (AOR, 4.0; 95% CI, 1.704, 9.196), having khat chewing family member (AOR, 2.87; 95% CI, 1.161, 7.070), poor school substance use controlling rule (AOR, 6.64; 95% CI, 1.863, 23.687), availability of substance retailing shops in residential areas (AOR, 2.9; CI, 1.303, 6.606), strong relationship with parents (AOR, 0.005; 95% CI, 0.001, 0.026), and being member of school mini-media (AOR, 0.177; 95% CI, 0.048, 0.657).

Conclusion

According to the findings of this study, one-quarter of the study participants were substance users. Alcohol, khat, and cigarettes were all commonly used substances. Gender, parent-child relationship, family member substance use history, school substance use controlling rules, school mini-media and pro-social involvement, and the availability of substance retailing shops were all strongly associated with substance use. Strengthening school rules on substance use, controlling substance retailing shops near schools and residential areas, and providing students with health education are all strategies for reducing substance use among students.
Mathematical modelling of SigE regulatory network reveals new insights into bistability of mycobacterial stress response
Authors: Zorzan Irene , Del Favero Simone , Giaretta Alberto , Manganelli Riccardo , Di Camillo Barbara , Schenato Luca
Published: November 2021

Background

The ability to rapidly adapt to adverse environmental conditions represents the key of success of many pathogens and, in particular, of Mycobacterium tuberculosis. Upon exposition to heat shock, antibiotics or other sources of stress, appropriate responses in terms of genes transcription and proteins activity are activated leading part of a genetically identical bacterial population to express a different phenotype, namely to develop persistence. When the stress response network is mathematically described by an ordinary differential equations model, development of persistence in the bacterial population is associated with bistability of the model, since different emerging phenotypes are represented by different stable steady states.

Results

In this work, we develop a mathematical model of SigE stress response network that incorporates interactions not considered in mathematical models currently available in the literature. We provide, through involved analytical computations, accurate approximations of the system’s nullclines, and exploit the obtained expressions to determine, in a reliable though computationally efficient way, the number of equilibrium points of the system.

Conclusions

Theoretical analysis and perturbation experiments point out the crucial role played by the degradation pathway involving RseA, the anti-sigma factor of SigE, for coexistence of two stable equilibria and the emergence of bistability. Our results also indicate that a fine control on RseA concentration is a necessary requirement in order for the system to exhibit bistability.
Genetic analysis of pharmacogenomic VIP variants in the Wa population from Yunnan Province of China
Authors: Li Dandan , Peng Linna , Xing Shishi , He Chunjuan , Jin Tianbo
Published: November 2021

Background

The variation of drug responses and target does among individuals is mostly determined by genes. With the development of pharmacogenetics and pharmacogenomics, the differences in drug response between different races seem to be mainly caused by the genetic diversity of pharmacodynamics and pharmacokinetics genes. Very important pharmacogenetic (VIP) variants mean that genes or variants play important and vital roles in drug response, which have been listed in pharmacogenomics databases, such as Pharmacogenomics Knowledge Base (PharmGKB). The information of Chinese ethnic minorities such as the Wa ethnic group is scarce. This study aimed to uncover the significantly different loci in the Wa population in Yunnan Province of China from the perspective of pharmacogenomics, to provide a theoretical basis for the future medication guidance, and to ultimately achieve the best treatment in the future.

Results

In this study, we recruited 200 unrelated healthy Wa adults from the Yunnan province of China, selected 52 VIP variants from the PharmGKB for genotyping. We also compared the genotype frequency and allele distribution of VIP variants between Wa population and the other 26 populations from the 1000 Genomes Project (http://www.1000Genomes.org/). Next, χ2test was used to determine the significant points between these populations. The study results showed that compared with the other 26 population groups, five variants rs776746 (CYP3A5), rs4291 (ACE), rs3093105(CYP4F2), rs1051298 (SLC19A1), and rs1065852 (CYP2D6) had higher frequencies in the Wa population. The genotype frequencies rs4291-TA, rs3093105-CA, rs1051298-AG and rs1065852-GA were higher than those of the other populations, and the allele distributions of rs4291-T and rs3093105-C were significantly different. Additionally, the difference between the Wa ethnic group and East Asian populations, such as CDX, CHB, and CHS, was the smallest.

Conclusions

Our research results show that there is a significant difference in the distribution of VIP variants between the Wa ethnic group and the other 26 populations. The study results will have an effect on supplementing the pharmacogenomics information for the Wa population and providing a theoretical basis for individualised medication for the Wa population.
Acute coronary syndrome with non-obstructive coronary arteries (ACS-NOCA) in patients with hypertrophic cardiomyopathy
Authors: Puwanant Sarinya , Trongtorsak Angkawipa , Wanlapakorn Chaisiri , Songsirisuk Nattakorn , Ariyachaipanich Aekarach , Boonyaratavej Smonporn
Published: November 2021

Objectives

Our study aimed to determine the prevalence and prognosis of acute coronary syndrome with non-obstructive coronary artery (ACS-NOCA) in patients with hypertrophic cardiomyopathy (HCM).

Methods and results

We enrolled a total of 200 consecutive patients with HCM over a 139-month period from 2002 to 2013. The study found that 28 patients (14% of overall patients, 51% of patients with ACS) had ACS-NOCA, and 18 patients (9% of overall patients, 86% of patients with acute MI) had MINOCA as initial clinical presentations. The highest prevalence of non-obstructive coronary artery disease (NOCA) in patients with HCM was found in acute ST-elevation myocardial infarction (STEMI) (100%), followed by non-STEMI (82%), and unstable angina (29%). Patients with ACS-NOCA had more frequent ventricular tachycardia and lower resting left ventricular (LV) outflow tract gradients than those with no ACS-NOCA (p < 0.05 for all). The ACS-NOCA group had a lower probability of HCM-related death compared with the no ACS-NOCA group and the significant coronary artery disease (CAD) group (p-log-rank = 0.0018).

Conclusions

MINOCA or ACS-NOCA is not an uncommon initial presentation (prevalence rate 9–14%) in patients with HCM. NOCA was highly prevalent (51–86%) in patients with HCM presenting with ACS and had a favorable prognosis. Our findings highlight as a reminder that in an era of rapid reperfusion therapy, ACS in patients with HCM is not only a result of obstructive epicardial CAD, but also stems from the complex cellular mechanisms of myocardial necrosis.
Fishing line assisted endoscopic placement of multiple plastic biliary stents for unresectable malignant hilar biliary obstruction: a retrospective study
Authors: Zhang Huahui , Li Fengdong , Huang Jian , Huo Chunyan , Huang Jin
Published: November 2021

Background and aims

Stent migration is one of the most common complications during the placement of multiple plastic biliary stents (MPBS) under endoscopy. This study aims to evaluate the feasibility and efficiency of the fishing line assisted (FLA) method for preventing the complication.

Methods

Patients with unresectable malignant hilar biliary obstruction (MHBO) who undergone endoscopic placement of MPBS using the FLA or conventional method from May 2018 to April 2021 in our center were enrolled in the study. The endpoints of this study were the stent migration rate, technical success rates, adverse events rates, times of stent migration, and the procedure time.

Results

FLA group (N = 19) and conventional group (N = 22) had similar baseline characteristics of the patients. The technical success rates (100% vs. 95.5%; P > 0.05), ERCP-related adverse events rates (5.3% vs. 4.5%; P > 0.05), and the stent-related adverse events rates (0% vs. 4.5%; P > 0.05) were no significant differences between the FLA and conventional groups. MPBS inserted using the conventional method consumed more time (median, 33.9 min vs. 15.6 min; P < 0.05) method and increased the times of stent migration (median, 3 times vs. 0 times; P < 0.05) than using the FLA method. Even if no statistical difference was detected in the stent migration rate between groups, this rate was lower in the FLA group than the conventional group (0% vs. 13.6%; P > 0.05).

Conclusions

FLA method is an effective technique for MPBS implantation to prevent stent migration during endoscopic retrograde cholangiography (ERCP). The method should be applied to patients with unresectable MHBO who need to place MPBS.
Tumor response as defined by iRECIST in gastrointestinal malignancies treated with PD-1 and PD-L1 inhibitors and correlation with survival
Authors: Xie Peiyi , Zheng Hong , Chen Haiyang , Wei Kaikai , Pan Ximin , Xu Qinmei , Wang Yongchen , Tang Changguan , Gevaert Olivier , Meng Xiaochun
Published: November 2021

Background

Atypical tumor response patterns during immune checkpoint inhibitor therapy pose a challenge to clinicians and investigators in immuno-oncology practice. This study evaluated tumor burden dynamics to identify imaging biomarkers for treatment response and overall survival (OS) in advanced gastrointestinal malignancies treated with PD-1/PD-L1 inhibitors.

Methods

This retrospective study enrolled a total of 198 target lesions in 75 patients with advanced gastrointestinal malignancies treated with PD-1/PD-L1 inhibitors between January 2017 and March 2021. Tumor diameter changes as defined by immunotherapy Response Evaluation Criteria in Solid Tumors (iRECIST) were studied to determine treatment response and association with OS.

Results

Based on the best overall response, the tumor diameter ranged from − 100 to + 135.3% (median: − 9.6%). The overall response rate was 32.0% (24/75), and the rate of durable disease control for at least 6 months was 30.7% (23/75, one (iCR, immune complete response) or 20 iPR (immune partial response), or 2iSD (immune stable disease). Using univariate analysis, patients with a tumor diameter maintaining a < 20% increase (48/75, 64.0%) from baseline had longer OS than those with ≥20% increase (27/75, 36.0%) and, a reduced risk of death (median OS: 80 months vs. 48 months, HR = 0.22, P = 0.034). The differences in age (HR = 1.09, P = 0.01), combined surgery (HR = 0.15, P = 0.01) and cancer type (HR = 0.23, P = 0.001) were significant. In multivariable analysis, patients with a tumor diameter with a < 20% increase had notably reduced hazards of death (HR = 0.15, P = 0.01) after adjusting for age, combined surgery, KRAS status, cancer type, mismatch repair (MMR) status, treatment course and cancer differentiation. Two patients (2.7%) showed pseudoprogression.

Conclusions

Tumor diameter with a < 20% increase from baseline during therapy in gastrointestinal malignancies was associated with therapeutic benefit and longer OS and may serve as a practical imaging marker for treatment response, clinical outcome and treatment decision making.
The genotypic and phenotypic impact of hypoxia microenvironment on glioblastoma cell lines
Authors: Macharia Lucy Wanjiku , Muriithi Wanjiru , Heming Carlos Pilotto , Nyaga Dennis Kirii , Aran Veronica , Mureithi Marianne Wanjiru , Ferrer Valeria Pereira , Pane Attilio , Filho Paulo Niemeyer , Moura-Neto Vivaldo
Published: November 2021

Background

Glioblastoma is a fatal brain tumour with a poor patient survival outcome. Hypoxia has been shown to reprogram cells towards a stem cell phenotype associated with self-renewal and drug resistance properties. Activation of hypoxia-inducible factors (HIFs) helps in cellular adaptation mechanisms under hypoxia. Similarly, miRNAs are known to be dysregulated in GBM have been shown to act as critical mediators of the hypoxic response and to regulate key processes involved in tumorigenesis.

Methods

Glioblastoma (GBM) cells were exposed to oxygen deprivation to mimic a tumour microenvironment and different cell aspects were analysed such as morphological changes and gene expression of miRNAs and survival genes known to be associated with tumorigenesis.

Results

It was observed that miR-128a-3p, miR-34-5p, miR-181a/b/c, were down-regulated in 6 GBM cell lines while miR-17-5p and miR-221-3p were upregulated when compared to a non-GBM control. When the same GBM cell lines were cultured under hypoxic microenvironment, a further 4–10-fold downregulation was observed for miR-34-5p, miR-128a-3p and 181a/b/c while a 3–6-fold upregulation was observed for miR-221-3p and 17-5p for most of the cells. Furthermore, there was an increased expression of SOX2 and Oct4, GLUT-1, VEGF, Bcl-2 and survivin, which are associated with a stem-like state, increased metabolism, altered angiogenesis and apoptotic escape, respectively.

Conclusion

This study shows that by mimicking a tumour microenvironment, miRNAs are dysregulated, stemness factors are induced and alteration of the survival genes necessary for the cells to adapt to the micro-environmental factors occurs. Collectively, these results might contribute to GBM aggressiveness.