Investigation on Selection, Improvement of Biomass Production and Protein Content in Duckweeds (Lemna Strains)
Authors: Basnet Ramesh ,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China, Du Anping , Tan Li , Guo Ling , Jin Yanglin , Yi Zhuolin , Huang Tianfang , Fang Yang , Zhao Hai ,   University of Chinese Academy of Sciences, Beijing, 100049, China,   Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   University of Chinese Academy of Sciences, Beijing, 100049, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   University of Chinese Academy of Sciences, Beijing, 100049, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   University of Chinese Academy of Sciences, Beijing, 100049, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   University of Chinese Academy of Sciences, Beijing, 100049, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   University of Chinese Academy of Sciences, Beijing, 100049, China,   Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China,   CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Published: July 2024

Duc kweeds are becoming an emerging plant protein source as it may be produced in a more efficient and eco-friendly manner than other major crops. Screening is essential for tapping the desired traits. We conducted the screening on 32 Lemna species and accessions available in the Gene Bank. It follows two steps screening, primary screening involved culturing in 1/5 strength Hoagland Solution (HS) for one week. Based on protein %, the best three strains were cultured two sets ammonium and nitrate nitrogen source. Based on both biomass and protein content, D0158 strain was identified as the best. Then, the strain was optimized in five different parameters. In our study, we found that a longer photoperiod resulted in greater biomass accumulation and a lower percentage of protein. There was a 6.51% increase in crude protein, rising from 30.97% to 33.13% and dry biomass growth rate increase of 54.48% from 4.35 to 6.72 g/m2/d. The findings can be used to enhance the duckweed indoor cultivation system. Additionally, this protocol can be applied to other duckweed strains for similar purposes.