bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of gingival crevicular fluid oxidative stress markers in school-aged children and teenagers with insufficient sleep
Authors: Yin Qianwen , Liu Chao , Bao Han , Li Size , Huang Zhuwei , Gu Deao , Xiong Liping , Miao Leiying
Published: December 2022

Background

Sleep is crucial for survival. Sleep deprivation causes ROS accumulation and, consequently, oxidative stress. The goal of the study was to evaluate gingival crevicular fluid (GCF) levels of the oxidative stress status hydrogen peroxide (H2O2), superoxide glutathione (GSH), and cellular oxidative damage marker malondialdehyde (MDA) in school-aged children and teenagers with insufficient sleep.

Methods

This study investigated sleep duration in 80 participants from two different developmental stages: school-aged children (6–13 years) and teenagers (14–17 years). GCF samples were obtained from all individuals, and samples were investigated to detect H2O2, GSH, and MDA levels using the micro method.

Results

Results reveal that GCF MDA and H2O2in school-age children and teenagers with insufficient sleep were significantly higher than in children with sufficient sleep. GCF GSH with insufficient sleep was insignificantly lower than in children with sufficient sleep. There was no significant difference between school-age and teenage populations.

Conclusion

Sleep deprivation causes increased levels of oxidative stress in gingival crevicular fluid, and adequate sleep is essential for maintaining redox balance.
The mediating effect of social support on the association between socioeconomic status and self-reported oral health status among the migrant elderly following children in Weifang, China: a cross-sectional study
Authors: Pang Mingli , Wang Jieru , Tian Tingting , Zhao Jinfeng , Jiang Xiaoxu , Li Hexian , Kong Fanlei
Published: December 2022

Background

Driven by population aging and the rapid urbanization in China, many migrant elderly following children (MEFC) moved to big cities to care for their grandchildren. The purpose of this study is to clarify the mediating effect of social support on the relationship between socioeconomic status (SES) and self-reported oral health status among the MEFC in Weifang, China.

Methods

Multistage cluster random sampling was used to select the participants and finally 613 MEFC were included in the survey. The Social Support Rating Scale (SSRS) and the Chinese version of the Geriatric Oral Health Assessment Index (GOHAI) scale were used for data collection. Descriptive analysis, Rao-Scott test, t-test and structural equation modeling (SEM) were conducted in this study.

Results

Mean score of GOHAI of the MEFC was 54.95 ± 6.47. The SES of MEFC exerted positive direct effect both on social support (standardized coefficient = 0.15) and self-reported oral health status (standardized coefficient = 0.22); social support exerted positive direct effect on self-reported oral health status (standardized coefficient = 0.17). Social support partially mediated the association between SES and self-reported oral health status [95% confidence interval (CI) 0.003–0.064,P < 0.05], and the mediating effect of social support accounted for 12.0% of the total effect.

Conclusions

Higher GOHAI score of MEFC indicated their better self-reported oral health status. MEFCs’ SES could exert positive effect both on social support and self-reported oral health status, while the mediating effect of social support between SES and self-reported oral health status of MEFC was established.
Mapping lifestyle medicine in undergraduate medical education: a lever for enhancing the curriculum
Authors: Essa-Hadad Jumanah , Rudolf Mary CJ , Mani Noah , Malatskey Lilach
Published: December 2022

Background

In 2017, the Bipartisan Policy Center called for inclusion of lifestyle medicine (LM) in undergraduate medical education. Recognizing the requirement that lifestyle medicine should be an integral and integrated part of the curriculum, we undertook an in-depth mapping process to determine the extent of LM teaching at our Faculty, staff perceptions and the impact on medical students.

Methods

The study utilized mixed methodology. In Phase 1 (Mapping) structured analysis of course syllabi were conducted followed by observation of teaching sessions throughout the pre-clinical and clinical years, recording content, the degree of coverage, and time allocated to LM Medicine. In Phase 2 (Impact and perceptions), students’ attitudes and confidence in LM counselling were ascertained by questionnaire (scale 1–4) on completion of second and fourth year of studies. Interviews were conducted with course coordinators.

Results

Phase 1: Students received 58 hours of LM teaching, 49 hours pre-clinical and 9 clinical; 42 hours were dedicated to theoretical knowledge and 16 hours to teaching practical skills related to lifestyle behavior change. Nutrition received the most attention (18 hours), alcohol, sleep, smoking and sexuality the least. On completion of the internal medicine rotation, students (n = 48) agreed that LM guidance should be part of the physician’s role and that patients expected their physicians to be role models (mean ± sd; 3.4 ± 0.7). Students were fairly confident about providing general LM counselling (3.3 ± 1.1); but less so for exercise (3.0 ± 1.2), nutrition (2.7 ± 1.1), stress (2.5 ± 1.0), sleep (2.2 ± 1.2), and sexuality (2.1 ± 1.2). Staff recognized the importance of LM but reported time limitations and the need to bring in external experts to teach LM as challenges.

Conclusions

Real-time mapping of teaching is a valuable way to ascertain teaching in practice. Based on our mapping process, redesign of curricula is needed to integrate more competency-based, experiential teaching, particularly in the clinical years.
Mutations in SARS-CoV-2 structural proteins: a global analysis
Authors: Abavisani Mohammad , Rahimian Karim , Mahdavi Bahar , Tokhanbigli Samaneh , Mollapour Siasakht Mahsa , Farhadi Amin , Kodori Mansoor , Mahmanzar Mohammadamin , Meshkat Zahra
Published: December 2022

Background

Emergence of new variants mainly variants of concerns (VOC) is caused by mutations in main structural proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Therefore, we aimed to investigate the mutations among structural proteins of SARS-CoV-2 globally.

Methods

We analyzed samples of amino-acid sequences (AASs) for envelope (E), membrane (M), nucleocapsid (N), and spike (S) proteins from the declaration of the coronavirus 2019 (COVID-19) as pandemic to January 2022. The presence and location of mutations were then investigated by aligning the sequences to the reference sequence and categorizing them based on frequency and continent. Finally, the related human genes with the viral structural genes were discovered, and their interactions were reported.

Results

The results indicated that the most relative mutations among the E, M, N, and S AASs occurred in the regions of 7 to 14, 66 to 88, 164 to 205, and 508 to 635 AAs, respectively. The most frequent mutations in E, M, N, and S proteins were T9I, I82T, R203M/R203K, and D614G. D614G was the most frequent mutation in all six geographical areas. Following D614G, L18F, A222V, E484K, and N501Y, respectively, were ranked as the most frequent mutations in S protein globally. Besides, A-kinase Anchoring Protein 8 Like (AKAP8L) was shown as the linkage unit between M, E, and E cluster genes.

Conclusion

Screening the structural protein mutations can help scientists introduce better drug and vaccine development strategies.
Effect of cervical spine motion on displacement of posterolateral annulus fibrosus in cervical spondylotic radiculopathy with contained posterolateral disc herniation: a three-dimensional finite element analysis
Authors: Ye Lin-qiang , Chen Chao , Liu Yuan-hui , Li Zhen , Lu Guo-liang
Published: December 2022

Background

Previous studies on dynamic impingement of nerve root in cervical spondylotic radiculopathy (CSR) have focused on effect of cervical spine motion (CSM) on dimensional changes of intervertebral foramen. However, there are few studies to investigate effect of CSM on displacement of posterolateral intervertebral disc until now. The present study aimed to investigate effect of CSM on displacement of posterolateral annulus fibrosus (AF) in CSR with contained posterolateral disc herniation.

Methods

A C5–C6 CSR finite element model with unilateral contained posterolateral disc herniation was generated based on validated C5–C6 normal finite element model. Forward and backward displacement distributions of posterolateral AFs in CSR model and normal model were compared. Changes in forward and backward displacement magnitudes of posterolateral AFs of the herniated side and the healthy side in CSR model, with respect to those of the ipsilateral posterolateral AFs in normal model, were compared. The comparisons were performed under flexion, extension, lateral bendings and axial rotations.

Results

There was no difference in deformation trend of posterolateral AF between CSR model and normal model. Bilateral posterolateral AFs mainly moved forward during flexion and backward during extension. Left posterolateral AF mainly moved backward and right posterolateral AF forward during left lateral bending and left axial rotation. Left posterolateral AF mainly moved forward and right posterolateral AF backward during right lateral bending and right axial rotation. However, with respect to forward and backward displacement magnitudes of the ipsilateral posterolateral AFs in normal model, those of the herniated side increased relatively significantly compared with those of the healthy side in CSR model.

Conclusions

Flexion, lateral bending to the healthy side and axial rotation to the healthy side make posterolateral AF of the herniated side mainly move forward, whereas extension, lateral bending to the herniated side and axial rotation to the herniated side make it mainly move backward. These data may help select CSM or positions to diagnose and treat CSR with contained posterolateral disc herniation. Increase in deformation amplitude of posterolateral AF of the herniated side may also be the reason for dynamic impingement of nerve root in CSR with contained posterolateral disc herniation.
Five critically ill pregnant women/parturients treated with extracorporeal membrane oxygenation
Authors: Li Ying , Xu Chi , Li Furong , Yan Zheng , Ye Shugao , Ma Jinqi , Wen Juan
Published: December 2022

Background

Maternal mortality has always been a major medical concern. Recently, the successful application of extracorporeal membrane oxygenation (ECMO) technology in the rescue of near-death patients has been reported.

Case presentation

This study retrospectively analyzed 5 cases of critically ill pregnant women/parturients treated with ECMO for respiratory and circulatory failure in the Wuxi People’s Hospital from 2018 to 2020. The mean age of the 5 cases was 30.2 years. Among them, Cases 1 and 5 were treated with Venoarterial (VA) ECMO. Case 1 was diagnosed with congenital heart disease, atrial septal defect, and severe pulmonary hypertension. VA ECMO was applied before cesarean section and was successfully removed after double lung transplantation, but the patient died 10 months after delivery from lung infection. While Case 5 was diagnosed with systemic lupus erythematosus, lupus nephritis, thrombotic vascular disease, HELLP syndrome, and cerebral hemorrhage. VA ECMO was applied 39 days after cesarean section, and the patient died 40 days after delivery due to multiple organ failure. Cases 3 and 4 were treated with Venovenous (VV) ECMO. Case 3 was diagnosed with refractory postpartum hemorrhage, and Case 4 was diagnosed with postpartum hypoglycemic coma, aspiration pneumonia, and shock. They were treated with VV ECMO after delivery, and all survived after successful evacuation. Another Case (Case 2) was diagnosed with postpartum pelvic infection, sepsis and septic shock, and was treated with VA ECMO at 15 days after delivery. The patient changed to VV ECMO at 30 days after delivery due to significant improvement in heart function and poor lung function, but eventually died of multiple organ failure. For the 5 cases, the mean duration of ECMO was 8.7 days, the mean duration of intensive care was 22.0 days, and the mean length of hospital stay was 57.6 days. As a result, 3 patients gradually returned to normal with significant improvement in ventilation and oxygenation after ECMO treatment.

Conclusions

ECMO technology can be used to treat some of the critical obstetric patients with respiratory and circulatory failure that is ineffective to conventional treatment, but it has no therapeutic effect on the primary disease.
Age and environmental factors predict psychological symptoms in adolescent refugees during the initial post-resettlement phase
Authors: Hocking Debbie C. , Sundram Suresh
Published: December 2022

Background

Adolescent refugees are at high risk of developing mental disorders but are often not recognised early. This pilot study aimed to identify early putative risk factors associated with psychological symptoms in newly resettled refugee youth at potential risk of subsequently developing mental disorders.

Methods

Newly resettled adolescent refugees were recruited through English language schools in Melbourne, Australia. Participants were assessed with the MINI-Kid, Achenbach Youth Self-Report and Reaction of Adolescents to Traumatic Stress scale. Parents completed a mental health screening separately. Linear regression models were used to identify predictive factors associated with symptom ratings.

Results

Seventy-eight, ostensibly well, refugee adolescents (mean age = 15.0 ± 1.6 years) resettled in Australia for 6.1 ± 4.2 months were assessed. Levels of anxiety, depression and post-traumatic stress symptoms were considerably lower than in mainstream population data. Prior displacement was a key determinant of symptomatology. Transitory displacement, irrespective of duration, was associated with elevated scores for depression (t (47) = -4.05, p < 0.0001), avoidance/numbing (U = 466, p < .05) and total trauma (U = 506, p < .05) symptoms. Older age was a unique predictor of depression (F (1,74) = 8.98, p < .01), internalising (F(1,74) = 6.28, p < .05) and total (F(1,74) = 4.10, p < .05) symptoms, whilst parental depression symptoms (t = 2.01, p < 0.05), displacement (t = 3.35, p < 0.01) and, expectedly, trauma exposure (t = 3.94, p < 0.001) were unique predictors of post-traumatic stress symptoms.

Conclusions

Displaced status, older age, and parental symptoms predicted psychological symptoms in adolescent refugees in an initial relatively asymptomatic post-resettlement phase. The early recognition of at-risk refugee youth may provide an opportunity for preventative mental health interventions.
Pivotal role of High-Mobility Group Box 2 in ovarian folliculogenesis and fertility
Authors: Shirouzu Shinichiro , Sugita Naohiro , Choijookhuu Narantsog , Yamaguma Yu , Takeguchi Kanako , Ishizuka Takumi , Tanaka Mio , Fidya  , Kai Kengo , Chosa Etsuo , Yamashita Yoshihiro , Koshimoto Chihiro , Hishikawa Yoshitaka
Published: December 2022

Background

High-Mobility Group Box 1 (HMGB1) and HMGB2 are chromatin-associated proteins that belong to the HMG protein family, and are involved in the regulation of DNA transcription during cell differentiation, proliferation and regeneration in various tissues. However, the role of HMGB2 in ovarian folliculogenesis is largely unknown.

Methods

We investigated the functional role of HMGB1 and HMGB2 in ovarian folliculogenesis and fertilization using C57BL/6 wild type (WT) and HMGB2-knockout (KO) mice. Ovarian tissues were obtained from WT and HMGB2-KO mice at postnatal days 0, 3, 7, and 2, 6 months of age, then performed immunohistochemistry, qPCR and Western blotting analyses. Oocyte fertilization capability was examined by natural breeding and in vitro fertilization experiments.

Results

In HMGB2-KO mice, ovary weight was decreased due to reduced numbers of oocytes and follicles. Natural breeding and in vitro fertilization results indicated that HMGB2-KO mice are subfertile, but not sterile. Immunohistochemistry showed that oocytes expressed HMGB2, but not HMGB1, in neonatal and adult WT ovaries. Interestingly, in HMGB2-KO ovaries, a compensatory increase in HMGB1 was found in oocyte nuclei of neonatal and 2-month-old mice; however, this was lost at 6 months of age.

Conclusions

The depletion of HMGB2 led to alterations in ovarian morphology and function, suggesting that HMGB2 plays an essential role in ovarian development, folliculogenesis and fertilization.
Epigenetics of type 2 diabetes and diabetes-related outcomes in the Strong Heart Study
Authors: Domingo-Relloso Arce , Gribble Matthew O. , Riffo-Campos Angela L. , Haack Karin , Cole Shelley A. , Tellez-Plaza Maria , Umans Jason G. , Fretts Amanda M. , Zhang Ying , Fallin M. Daniele , Navas-Acien Ana , Everson Todd M.
Published: December 2022

Background

The prevalence of type 2 diabetes has dramatically increased in the past years. Increasing evidence supports that blood DNA methylation, the best studied epigenetic mark, is related to diabetes risk. Few prospective studies, however, are available. We studied the association of blood DNA methylation with diabetes in the Strong Heart Study. We used limma, Iterative Sure Independence Screening and Cox regression to study the association of blood DNA methylation with fasting glucose, HOMA-IR and incident type 2 diabetes among 1312 American Indians from the Strong Heart Study. DNA methylation was measured using Illumina’s MethylationEPIC beadchip. We also assessed the biological relevance of our findings using bioinformatics analyses.

Results

Among the 358 differentially methylated positions (DMPs) that were cross-sectionally associated either with fasting glucose or HOMA-IR, 49 were prospectively associated with incident type 2 diabetes, although no DMPs remained significant after multiple comparisons correction. Multiple of the top DMPs were annotated to genes with relevant functions for diabetes including SREBF1, associated with obesity, type 2 diabetes and insulin sensitivity; ABCG1, involved in cholesterol and phospholipids transport; and HDAC1, of the HDAC family. (HDAC inhibitors have been proposed as an emerging treatment for diabetes and its complications.)

Conclusions

Our results suggest that differences in peripheral blood DNA methylation are related to cross-sectional markers of glucose metabolism and insulin activity. While some of these DMPs were modestly associated with prospective incident type 2 diabetes, they did not survive multiple testing. Common DMPs with diabetes epigenome-wide association studies from other populations suggest a partially common epigenomic signature of glucose and insulin activity.
THOR is a targetable epigenetic biomarker with clinical implications in breast cancer
Authors: Apolónio Joana Dias , Dias João S. , Fernandes Mónica Teotónio , Komosa Martin , Lipman Tatiana , Zhang Cindy H. , Leão Ricardo , Lee Donghyun , Nunes Nuno Miguel , Maia Ana-Teresa , Morera José L. , Vicioso Luis , Tabori Uri , Castelo-Branco Pedro
Published: December 2022

Background

Breast cancer (BC) is the most frequently diagnosed cancer and a leading cause of death among women worldwide. Early BC is potentially curable, but the mortality rates still observed among BC patients demonstrate the urgent need of novel and more effective diagnostic and therapeutic options. Limitless self-renewal is a hallmark of cancer, governed by telomere maintenance. In around 95% of BC cases, this process is achieved by telomerase reactivation through upregulation of the human telomerase reverse transcriptase (hTERT). The hypermethylation of a specific region within the hTERT promoter, termed TERT hypermethylated oncological region (THOR) has been associated with increased hTERT expression in cancer. However, its biological role and clinical potential in BC have never been studied to the best of our knowledge. Therefore, we aimed to investigate the role of THOR as a biomarker and explore the functional impact of THOR methylation status in hTERT upregulation in BC.

Results

THOR methylation status in BC was assessed by pyrosequencing on discovery and validation cohorts. We found that THOR is significantly hypermethylated in malignant breast tissue when compared to benign tissue (40.23% vs. 12.81%, P < 0.0001), differentiating malignant tumor from normal tissue from the earliest stage of disease. Using a reporter assay, the addition of unmethylated THOR significantly reduced luciferase activity by an average 1.8-fold when compared to the hTERT core promoter alone (P < 0.01). To further investigate its biological impact on hTERT transcription, targeted THOR demethylation was performed using novel technology based on CRISPR-dCas9 system and significant THOR demethylation was achieved. Cells previously demethylated on THOR region did not develop a histologic cancer phenotype in in vivo assays. Additional studies are required to validate these observations and to unravel the causality between THOR hypermethylation and hTERT upregulation in BC.

Conclusions

THOR hypermethylation is an important epigenetic mark in breast tumorigenesis, representing a promising biomarker and therapeutic target in BC. We revealed that THOR acts as a repressive regulatory element of hTERT and that its hypermethylation is a relevant mechanism for hTERT upregulation in BC.
Bariatric surgery-induced weight loss and associated genome-wide DNA-methylation alterations in obese individuals
Authors: Talukdar Fazlur Rahman , Escobar Marcillo David Israel , Laskar Ruhina Shirin , Novoloaca Alexei , Cuenin Cyrille , Sbraccia Paolo , Nisticò Lorenza , Guglielmi Valeria , Gheit Tarik , Tommasino Massimo , Dogliotti Eugenia , Fortini Paola , Herceg Zdenko
Published: December 2022

Background

Obesity is a multifactorial and chronic condition of growing universal concern. It has recently been reported that bariatric surgery is a more successful treatment for severe obesity than other noninvasive interventions, resulting in rapid significant weight loss and associated chronic disease remission. The identification of distinct epigenetic patterns in patients who are obese or have metabolic imbalances has suggested a potential role for epigenetic alterations in causal or mediating pathways in the development of obesity-related pathologies. Specific changes in the epigenome (DNA methylome), associated with metabolic disorders, can be detected in the blood. We investigated whether such epigenetic changes are reversible after weight loss using genome-wide DNA methylome analysis of blood samples from individuals with severe obesity (mean BMI ~ 45) undergoing bariatric surgery.

Results

Our analysis revealed 41 significant (Bonferronip < 0.05) and 1169 (false discovery ratep < 0.05) suggestive differentially methylated positions (DMPs) associated with weight loss due to bariatric surgery. Among the 41 significant DMPs, 5 CpGs were replicated in an independent cohort of BMI-discordant monozygotic twins (the heavier twin underwent diet-induced weight loss). The effect sizes of these 5 CpGs were consistent across discovery and replication sets (p < 0.05). We also identified 192 differentially methylated regions (DMRs) among whichSMAD6andPFKFB3genes were the top hypermethylated and hypomethylated regions, respectively. Pathway enrichment analysis of the DMR-associated genes showed that functional pathways related to immune function and type 1 diabetes were significant. Weight loss due to bariatric surgery also significantly decelerated epigenetic age 12 months after the intervention (mean =  − 4.29;p = 0.02).

Conclusions

We identified weight loss-associated DNA-methylation alterations targeting immune and inflammatory gene pathways in blood samples from bariatric-surgery patients. The top hits were replicated in samples from an independent cohort of BMI-discordant monozygotic twins following a hypocaloric diet. Energy restriction and bariatric surgery thus share CpGs that may represent early indicators of response to the metabolic effects of weight loss. The analysis of bariatric surgery-associated DMRs suggests that epigenetic regulation of genes involved in endothelial and adipose tissue function is key in the pathophysiology of obesity.
Radiomics assessment of carotid intraplaque hemorrhage: detecting the vulnerable patients
Authors: Zhang Shuai , Gao Lin , Kang Bing , Yu Xinxin , Zhang Ran , Wang Ximing
Published: December 2022

Background

Intraplaque hemorrhage (IPH), one of the key features of vulnerable plaques, has been shown to be associated with increased risk of stroke. The aim is to develop and validate a CT-based radiomics nomogram incorporating clinical factors and radiomics signature for the detection of IPH in carotid arteries.

Methods

This retrospective study analyzed the patients with carotid plaques on CTA from January 2013 to January 2021 at two different institutions. Radiomics features were extracted from CTA images. Demographics and CT characteristics were evaluated to build a clinical factor model. A radiomics signature was constructed by the least absolute shrinkage and selection operator method. A radiomics nomogram combining the radiomics signature and independent clinical factors was constructed. The area under curves of three models were calculated by receiver operating characteristic analysis.

Results

A total of 46 patients (mean age, 60.7 years ± 10.4 [standard deviation]; 36 men) with 106 carotid plaques were in the training set, and 18 patients (mean age, 61.4 years ± 10.1; 13 men) with 38 carotid plaques were in the external test sets. Stenosis was the independent clinical factor. Eight features were used to build the radiomics signature. The area under the curve (AUC) of the radiomics nomogram was significantly higher than that of the clinical factor model in both the training (p = 0.032) and external test (p = 0.039) sets.

Conclusions

A CT-based radiomics nomogram showed satisfactory performance in distinguishing carotid plaques with and without intraplaque hemorrhage.
Isoflurane vs. propofol for sedation in invasively ventilated patients with acute hypoxemic respiratory failure: an a priori hypothesis substudy of a randomized controlled trial
Authors: Becher Tobias , Meiser Andreas , Guenther Ulf , Bellgardt Martin , Wallenborn Jan , Kogelmann Klaus , Bracht Hendrik , Falthauser Andreas , Nilsson Jonas , Sackey Peter , Kellner Patrick
Published: December 2022

Background

Acute hypoxemic respiratory failure (AHRF) is a leading concern in critically ill patients. Experimental and clinical data suggest that early sedation with volatile anesthestics may improve arterial oxygenation and reduce the plasma and alveolar levels of markers of alveolar epithelial injury and of proinflammatory cytokines.

Methods

An a priori hypothesis substudy of a multicenter randomized controlled trial (The Sedaconda trial, EUDRA CT Number 2016-004551-67). In the Sedaconda trial, 301 patients on invasive mechanical ventilation were randomized to 48 h of sedation with isoflurane or propofol in a 1:1 ratio. For the present substudy, patients with a ratio of arterial pressure of oxygen (PaO2) to inspired fraction of oxygen (FiO2), PaO2/FiO2, of ≤ 300 mmHg at baseline were included (n = 162). The primary endpoint was the change in PaO2/FiO2 between baseline and the end of study sedation. A subgroup analysis in patients with PaO2/FiO2 ≤ 200 mmHg was performed (n = 82).

Results

Between baseline and the end of study sedation (48 h), oxygenation improved to a similar extent in the isoflurane vs. the propofol group (isoflurane: 199 ± 58 to 219 ± 76 mmHg (n = 70), propofol: 202 ± 62 to 236 ± 77 mmHg (n = 89); p = 0.185). On day seven after randomization, PaO2/FiO2 was 210 ± 79 mmHg in the isoflurane group (n = 41) and 185 ± 87 mmHg in the propofol group (n = 44; p = 0.411). In the subgroup of patients with PaO2/FiO2 ≤ 200 mmHg, PaO2/FiO2 increase between baseline and end of study sedation was 152 ± 33 to 186 ± 54 mmHg for isoflurane (n = 37), and 150 ± 38 to 214 ± 85 mmHg for propofol (n = 45; p = 0.029). On day seven, PaO2/FiO2 was 198 ± 69 mmHg in patients randomized to isoflurane (n = 20) and 174 ± 106 mmHg in patients randomized to propofol (n = 20; p = 0.933). Both for the whole study population and for the subgroup with PaO2/FiO2 ≤ 200 mmHg, no significant between-group differences were observed for PaCO2, pH and tidal volume as well as 30-day mortality and ventilator-free days alive.

Conclusions

In patients with AHRF, inhaled sedation with isoflurane for a duration of up to 48 h did not lead to improved oxygenation in comparison to intravenous sedation with propofol.

Trial registration The main study was registered in the European Medicines Agency’s EU Clinical Trial register (EudraCT), 2016-004551-67, before including the first patient. The present substudy was registered at German Clinical Trials Register (DRKS, ID: DRKS00018959) on January 7th, 2020, before opening the main study data base and obtaining access to study results.
Weather and crime: a systematic review of the empirical literature
Authors: Corcoran Jonathan , Zahnow Renee
Published: December 2022

The weather-crime association has intrigued scholars for more than 150 years. While there is a long-standing history of scholarly interest in the weather-crime association, the last decade has evidenced a marked increase in the volume and diversity of empirical studies concerned with weather and its social implications including crime. In this paper we conduct a review of the empirical literature on weather and crime. This is the first review on the topic for more than 30 years. We highlight the theoretic and empirical challenges that persist and act to impede our understanding and chart four interrelated thematic areas that require concerted attention. These are to: (1) strengthen and integrate theoretical explanations of the weather-crime association; (2) model multi-dimensional processes of the weather-crime association; (3) broaden the situational, climatic, and cultural contexts of study; and (4) to translate research into policy and practice. We conclude by advocating for scholarship to advance the four thematic areas such that we progress towards a more complete and robust understanding of the weather-crime association.
The impairment of intramural periarterial drainage in brain after subarachnoid hemorrhage
Authors: Sun Yanrong , Liu E. , Pei Yanhong , Yao Qinhan , Ma Haowen , Mu Yakun , Wang Yingjie , Zhang Yan , Yang Xiaomei , Wang Xing , Xue Jiajia , Zhai Jiliang , Carare Roxana O. , Qin Lihua , Yan Junhao
Published: December 2022

Interstitial fluid (ISF) from brain drains along the basement membranes of capillaries and arteries as Intramural Periarterial Drainage (IPAD); failure of IPAD results in cerebral amyloid angiopathy (CAA). In this study, we test the hypothesis that IPAD fails after subarachnoid haemorrhage (SAH). The rat SAH model was established using endovascular perforation method. Fluorescence dyes with various molecular weights were injected into cisterna magna of rats, and the pattern of IPAD after SAH was detected using immunofluorescence staining, two-photon fluorescent microscope, transmission electron microscope and magnetic resonance imaging tracking techniques. Our results showed that fluorescence dyes entered the brain along a periarterial compartment and were cleared from brain along the basement membranes of the capillaries, with different patterns based on individual molecular weights. After SAH, there was significant impairment in the IPAD system: marked expansion of perivascular spaces, and ISF clearance rate was significantly decreased, associated with the apoptosis of endothelial cells, activation of astrocytes, over-expression of matrix metalloproteinase 9 and loss of collagen type IV. In conclusion, experimental SAH leads to a failure of IPAD, clinically significant for long term complications such as CAA, following SAH.
Actualizing community–academic partnerships in research: a case study on rural perinatal peer support
Authors: Hards April , Cameron Audrey , Sullivan Eva , Kornelsen Jude
Published: December 2022

Background

 Within the field of patient and public involvement in health service research, there is a growing movement towards not only involving patients in research but engaging them as co-producers of knowledge. We explore such a co-productive research relationship in a case study on rural perinatal mental health, with the aim of collaboratively developing knowledge based on both the relevant lived experience of a community partner, and the systemic knowledge of academic researchers.

Methods

 Data was gathered through a community forum and subsequent interviews with social service program administrators from rural British Columbia, Canada. Interviews were analyzed separately by the community partner and academic researchers using principles of thematic analysis. Both the community partner and academic researchers were involved from project genesis to data collection, analysis, interpretation, and manuscript writing.

Results

Common themes identified by the academic and community researchers included needs for peer support, barriers to peer support, and gaps in mental health care. Divergently, the academic researcher focused on systems-level challenges while the community partner emphasized the impact of power dynamics within health systems. Researchers generated five methodological values propositions from the process of co-production, including (a) mutual respect for all viewpoints, (b) a rejection of assumed hierarchy, (c) commitments to truth speaking, (d) attention to process, and (e) equivalence of contribution.

Conclusions

Co-production highlights the value of lived experience in health research, sets it in conversation with scientific inquiry, and moves away from hierarchies of assumed knowledge often embedded in traditional health care research. Incorporating both academic researcher and community partner writing into our paper reflects a commitment to maintaining the integrity and authenticity of lived experience, an affirmation of its equal validity as a source of knowledge, and a rejection of qualifying patient voices. The exploration of this co-production research relationship lays groundwork for future research teams considering collaborative methodology. We suggest co-productive research as a means of addressing the epistemic injustice that arises in health care research from the privileging of certain forms of knowledge, and the exclusion of others, namely that derived from patient experience.
Prognostic value of biological parameters in patients with severe aortic stenosis undergoing TAVI - results at six months
Authors: Dumitrof Luiza Cristina , Roca Mihai , Mihăescu Traian , Tinică Grigore
Published: December 2022

(1) Background: Aortic stenosis is the most common valvulopathy in elderly patients over 60 years of age. The absence of immediate surgical intervention through classic valve replacement or through a minimally invasive procedure, namely transcatheter implantation of the aortic valve (TAVI) leads to an increase in the risk of morbidity and mortality through the deterioration of the clinical condition. Since the first interventional transcatheter aortic valve implantation procedure was performed in 2012 and until now, the progression of medical technology and state-of-the-art valves have led to the refinement of the treatment strategy and the improvement of the prognosis of patients with aortic stenosis undergoing TAVI in the first 6 months after the procedure; (2) Methods: We conducted a prospective study in which 86 patients diagnosed with severe aortic stenosis underwent minimally invasive valve replacement by TAVI; (3) Results: The presence of preoperative anemia is associated with a negative prognosis both in the medium term and in evolution, and in addition to hematological parameters, we also evaluated a series of biochemical data, with special attention to renal function and lipid profile; (4) Conclusions: Biological parameters followed after TAVI may be predictors associated with a negative long-term prognosis.
Examining of some physical and physiological parameters of 10-18 years old male skiers to seasonal cycles
Authors: Budak Cemalettin , Dündar Sibel Tetik , Mertoğlu Cuma
Published: December 2022

Abstract: Skiing can be identified as a branch with the most important representative power of winter sports. Further, it has been characterized by high popularity and population, branching off in itself. During the year, different physical performance and physiological indicators are sometimes encountered in athletes depending on seasonal cycles, training levels, and living conditions. The aim of this study is to examine some physical performance parameters and the determined hormone levels to seasonal cycles. Methods: 15 male skiers with a mean age of 14.53±2.61 (years), a mean height of 158.53±9.66 (cm), and a mean body mass of 54.20±10.85 (kg) participated in the study. The information about participants' age, height, and body mass was determined by standard methods. Various measurement tools were used including a digital hand dynamometer (TKK 5401) for hand grip strength, a digital dynamometer (TKK 5402) for back and leg strength, a jump meter (Takei TKK 5406) for vertical jump height, and an electronic hand spirometer (firstMED) for respiratory functions. The Wingate anaerobic power test (Monark 894 E bicycle ergometer) was performed to determine the anaerobic power level. Additionally, to determine somatotypes (ectomorph, mesomorph, endomorph) and body fat percentage; skinfold caliper (Holtain), tape measure, and digital caliper (Holtain) were respectively utilized for skinfold thickness measurement, circumference measurements, and diameter measurements. Blood samples (hemogram test, vitamin D, cortisol, and testosterone to be checked) were taken from the antecubital vein in the sitting position. From the blood samples, serum plasma was separated and preserved by centrifugation (+4o) and all samples were analyzed at once. All tests were performed once in September, December, March, and June at an altitude of 2,000 and in pre-season and mid-season. The data were analyzed through IBM SPSS 24.0 package program. Shapiro-Wilk was used to determine the distribution of the data, descriptive and frequency analysis was used to determine the mean of the variables, and one-way analysis of variance (ANOVA) was used to determine the differences between measurements. The results were presented as arithmetic mean and standard deviation (𝑋̅±Ss). Results: In 10-18 years old male skiers, it was determined that vitamin D reached its highest level in autumn, testosterone in summer, and cortisol in winter. In the inter-test comparison results, significant differences were determined in the vertical jump, right and left-hand grip strength, leg strength, testosterone, vitamin D, HCT, FVC, FEV1, and anaerobic power parameters. Conclusions: The results of the study have mostly supported the literature.
Keywords: vitamin D; testosterone; cortisol; anaerobic power; respiratory functions
The impact of perception regarding therapeutic exercises and dietary changing adherence of subjects known with low back pain
Authors: Murgoci Nicolae
Published: December 2022

Abstract: Debates regarding the role of therapeutic exercises and diet as modulators of an anti-inflammatory state occurred in the last years in the medical environment. The syner-gy between moderate-intensity exercise and a proper diet targeting decreasing IL-1 inhib-its the production of the pro-inflammatory cytokine TNF-α, the key regulator of local and systemic inflammation. One of the most important causes of short and long-term disabil-ity in all occupational groups is back pain, impacting the quality of life. Degeneration of the intervertebral disc (IVD) causes low back pain that intensifies with age. Assessment of the Oswestry Disability Index was applied on 23 subjects with low back pain to investigate the degree of disability. Nutrition of IVD, implying therapeutic exercises, and a customized diet may be crucial adjuvants for the rehabilitation process. The appropriate diet and therapeutic exercise approach are meant to evaluate the impact of awareness regarding the possibility of improving health outcomes. In this present study, women have a strong positive Pearson correlation (p<0.05) with minimal (66-70 years) disability and moderate disability (r=1.000, CI =99%). Subjects with moderate disability conditions have “no” intention to implement diet changes and maintain therapeutic exercise adher-ence (r=0.902, CI = 95%). Men (71-75 years, r=0.995, CI =99%) registered a positive strong correlation with maximum deficiency (r=1.000, CI =99%) and “possible no” change in diet and exercise adherence will be applied (r=0.866, CI = 95%). Total disability responders an-swered with a “probable yes” option (r=0.884, CI=95%) but the dependence on their ca-reers is decisive. The education strategy is essential because diet change implementation can cause resistive behavior as well as adherence to exercise therapy. A key to effectively managing the inflammatory state due to different comorbidities is to use the cumulative effects of health professionals' prescriptions. The challenge is to ensure adherence to these actions for each patient.
Keywords: anti-inflammatory, therapeutic exercises, rehabilitation, diet, Oswestry Disability In-dex (ODI), back pain, intervertebral disc, nutrition, perception, disability.
Interactive device for the treatment of pediatric neuromotor deficiencies using personalized recovery programs
Authors: Băeșu Andra Cristiana , Fuior Robert , Luca Cătălina , Corciovă Călin
Published: December 2022

Abstract: Modern rehabilitation procedures use devices that provide physical therapists with various types of information to improve assessment of patient progress during reha-bilitation plans. The new trend of these technologies is the development of safe, portable and comfortable wearable devices with extensive applications in various environments (medical clinics or at the patient's home). The present work presents a portable and safe device for hand rehabilitation, consisting of five finger force sensors and a palmar sensor arranged in the ball, capable of capturing pressure signals during the execution of move-ments guided by the physiotherapist or by a video game/virtual reality. A 3-axis accel-erometer was used to spatially monitor the patient's movements. A series of games with different levels of difficulty were created, through which the degree of mobility of the pa-tient can be monitored depending on the game he chooses and at the same time reflected by the score obtained at the end of the game. Also, to be more interactive, the interface was chosen to play with 2 players simultaneously. So that they can choose to play in the team or as competitors. The system allows users to show different routines to guide them in their use and also evaluates pressure signals and response time. Keywords: microcontroller, games interactive, physiokinetotherapist, rehabilitation, health im-provement.
The role of the therapeutic physical exercise and the complex recovery treatment for the patients with chronic degenerative diseases during the COVID-19 pandemic
Authors: Silisteanu Andrei Emanuel , Antonescu Oana Raluca , Racheriu Mihaela
Published: December 2022

Abstract: Background: Osteoarthritis is one of the most common causes of pain and musculoskeletal disability and mainly affects the middle-aged and the elderly. The condition is chronic and disabling, it diminishes the patients' quality of life. Purpose. The study had the purpose of pointing out a possible connection between physical ac-tivity and the reduction of pain accompanied by the increased functional capacity in patients with chronic degenerative diseases during the COVID-19 pandemic. Material and method: The study was conducted on an outpatient basis for a period of 6 months on a number of 40 patients diagnosed with osteoarthritis. The evaluation of the pa-tients was made at the beginning of the treatment and at its end (after 4 weeks), as well as at the control after 12 weeks. Results: The study included a number of 40 patients over 45 years old, divided into 2 study groups: in group L1-knee osteoarthritis and in group L2 -hip osteoarthritis. The therapeutic physical exercise reduced pain and stiff-ness, and it also improved the functional capacity. Conclusions: Therapeutic physical exercise has an important role in the reduction of pain and disability, as well as in the increase in the quality of life, if it is done properly.
Keywords: osteoarthritis, therapeutic physical exercise, pain, pandemic COVID-19
Traumatology and functional recovery of the shoulder specific to the game of Volleyball
Authors: Ionite Catalin , Stirbu Ilie-Catalin , Stirbu Catalin-Mihaela , Condurache Iustina , Rotariu Mariana
Published: December 2022

Abstract: Worldwide, the game of volleyball is a very popular sport regardless of the demographic categories analyzed. In recent years, the dynamics of the game of volleyball is continuously increasing, notable increases being observed among the female gender. Shoulder injuries are common among athletes, for example in baseball, 12% to 19% of injuries encountered are located in the shoulder, while in swimming, the percentage of shoulder injuries is estimated to be between 23% and 38%. The research is based on the case study carried out on a 28-year-old male subject, with 15 years of sports experience, a volleyball player, with a previous history of shoulder joint through the presence of bicipital tendinitis, contractures in the trapezius muscle, as well as in the the sternocleidomastoid muscle. The analysis of the dynamics of the evolution of the subject included in the research and the interpretation of the results led to the confirmation of the hypothesis, so that, reaching all the well-established recovery objectives, we can conclude that the symptomatology, in our case, rotator cuff muscle injury, can be combated by using kinetotherapeutic methods , which demonstrated the highest level of success achieved, when the subject can be taken even from the immobilization stage. Also, a much faster progress was observed from the point of view of socio-professional reintegration.
Keywords: Volleyball, Shoulder, Recovery.
Child development with the D-score: turning milestones into measurement
Authors: van Buuren Stef , Eekhout Iris
Published: December 2022

The chapter equips the reader with a basic understanding of robust psychometric methods that are needed to turn developmental milestones into measurements, introducing the fundamental issues in defining a unit for child development and demonstrates the relevant quantitative methodology.

It reviews quantitative approaches to measuring child development;

introduces the Rasch model in a non-technical way;

shows how to estimate model parameters from real data;

puts forth a set of principles for model evaluation and assessment of scale quality;

analyses the relation between early D-scores and later intelligence;

and compares the D-scores from three studies that all use the same instrument.