bluuebunny/crossref_metadata_2025 huggingface dataset:
Wing morphology variations in Culicoides circumscriptus from France
Authors: Hadj-Henni Leila , Djerada Zoubir , Millot Christine , Cousinat Mireille , Lehrter Véronique , Augot Denis
Published: April 2023

The biting midge Culicoides circumscriptus Kieffer, 1918 is a European widespread vector of avian malaria throughout the continent and is a possible vector of Akabane virus and Bluetongue virus. This species populates a wide range of environments in contrasting ecological settings often exposed to strong seasonal fluctuations. The main goals of this study were to investigate C. circumscriptus phenotypic variation at three departments in France (Corsica Island, Moselle and Var) and to determine if its phenotypes vary with the environment. Culicoides circumscriptus wing phenotypes were analyzed using a geometric morphometric approach based on anatomical landmarks and outlines of the wing. Dendogram trees based on landmarks and the outlines-2 set (cell m4) showed similar topologies and separated populations of C. circumscriptus. In contrast, another set of outlines-1 (covering the r-m cross vein, M, radiale and arculus) presented a different hierarchical clustering tree. The phenotypic variation observed in C. circumscriptus indicated that these populations are exposed to environmental and ecological pressures. Our results suggest the presence of phenotypic plasticity in this species.
Model Identification and Control of a Buoyancy Change Device
Authors: Carneiro João Falcão , Pinto J. Bravo , Almeida F. Gomes de , Cruz N. A.
Published: April 2023

There are several compelling reasons for exploring the ocean, for instance, the potential for accessing valuable resources, such as energy and minerals; establishing sovereignty; and addressing environmental issues. As a result, the scientific community has increasingly focused on the use of autonomous underwater vehicles (AUVs) for ocean exploration. Recent research has demonstrated that buoyancy change modules can greatly enhance the energy efficiency of these vehicles. However, the literature is scarce regarding the dynamic models of the vertical motion of buoyancy change modules. It is therefore difficult to develop adequate depth controllers, as this is a very complex task to perform in situ. The focus of this paper is to develop simplified linear models for a buoyancy change module that was previously designed by the authors. These models are experimentally identified and used to fine-tune depth controllers. Experimental results demonstrate that the controllers perform well, achieving a virtual zero steady-state error with satisfactory dynamic characteristics.
Power Saving in Magnetorquers by Operating in Cryogenic Environments
Authors: Villalba-Alumbreros Gabriel , Lopez-Pascual Diego , Valiente-Blanco Ignacio , Diez-Jimenez Efren
Published: April 2023

Satellites with cryogenic instrumentation have great potential for military, commercial, and scientific space missions due to the increased sensitivity of their sensors, even for Low Earth Orbit (LEO) missions. For these missions, magnetorquers are a common electromagnetic actuation solution for controlling the attitude and orientation of the satellite. As for any other component of a satellite, the optimization of power consumption and weight is always beneficial for the design. In this work, we propose a novel idea to reduce power consumption during magnetorquer operation: installing the magnetorquer in the cryogenic area of the satellite, instead of installing an actuator in the hot area. As the electric resistivity of the wire is greatly reduced, power consumption is also reduced. However, the heat generated in the magnetorquer, even if lower, must still be dissipated by the cryocooling system, which has an additional energetic cost. The cryogenic temperature range where this effect is beneficial, and the amount of power saved, was determined as a function of different cryocooler technologies’ efficiency and the purity of the copper wire material. It is analytically demonstrated that the operation of the magnetorquer in a temperature range from 10 to 40 K could save energy with respect to operation at 300 K if the copper wires have a residual resistance ratio larger than 200 RRR. A prototype magnetorquer suitable for cryogenic temperatures was manufactured and tested at liquid nitrogen temperature, 77 K, to experimentally demonstrate the variation in the energy consumption. The magnetorquer comprised an iron core with copper wire winding that achieved 1.42 Am2 by applying 0.565 W at 0.5 A. When operating submerged in liquid nitrogen at a temperature of 77 K, the power used by the magnetorquer was reduced by eight times due to the change in electrical resistivity.
Organic Nitrogen Fertilizer Selection Influences Water Use Efficiency in Drip-Irrigated Sweet Corn
Authors: Sukor Arina , Qian Yaling , Davis Jessica G.
Published: April 2023

Organic farmers often rely on off-farm nitrogen (N) sources for mid-season N. Farmers can also produce cyano-fertilizer on-farm by growing N-fixing cyanobacteria (Anabaena spp.) in raceways and applying the cyanobacteria through irrigation systems. A two-year field study was conducted, and blood meal, feather meal, fish emulsion, and cyano-fertilizer were evaluated to determine whether the water use efficiency (WUE) of sweet corn (Zea mays) was affected by fertilizer type. Fish emulsion and cyano-fertilizer were supplied in four split applications through drip irrigation, while the blood meal and feather meal were subsurface banded pre-plant. Leaf gas exchange measurements were taken during tasseling. The amounts of phytohormone and Fe applied in organic N fertilizers were correlated with field water use efficiency (fWUE), instantaneous water use efficiency (iWUE), and leaf gas exchange components of sweet corn. A positive relationship was observed between the amount of salicylic acid (SA) applied with both iWUE (r = 0.71, p < 0.05) and fWUE (r = 0.68, p < 0.01). The amount of Fe applied was positively correlated with the leaf vapor pressure deficit (r = 0.54, p < 0.01) and transpiration rate (r = 0.53, p < 0.01). Cyano-fertilizer had the highest yield and WUE, likely due to the high amount of SA applied, although fish emulsion was comparable in year one. These relationships require further exploration to elucidate the mechanisms impacting WUE.
Selection of Faba Bean (Vicia faba L.) Genotypes for High Yield, Essential Amino Acids and Low Anti-Nutritional Factors
Authors: Akgun Didem , Canci Huseyin
Published: April 2023

One of the important edible legumes, the faba bean (Vicia faba L.) contains high protein levels and amino acids in its seeds essential for human nutrition and animal feeding; it also consists of anti-nutritional factors such as vicine, convicine, and tannin. For a balanced and healthy diet, faba bean cultivars should be improved for high seed yield, essential amino acids, and low anti-nutritional factors. The aims of this study were to select faba bean genotypes for (i) high yield, (ii) low anti-nutritional factors, and (iii) essential amino acids. A total of 12 faba bean genotypes, including 10 genotypes with low tannin content and 2 local checks, were assessed for phenological, morphological, and agronomical traits, as well as some biochemical characteristics including essential amino acids and low anti-nutritional factors. A local population, Atlidere, and a breeding line with low tannin content, FLIP08-016FB, had the highest yield. FLIP08-016FB had not only the highest lysine, methionine, and cysteine content, but also the highest yield and low anti-nutritional factors. FLIP08-016FB was selected for a balanced and healthy diet as it had a high seed yield, essential amino acids, and low anti-nutritional factors.
Modeling Topsoil Phosphorus—From Observation-Based Statistical Approach to Land-Use and Soil-Based High-Resolution Mapping
Authors: Kull Anne , Kikas Tambet , Penu Priit , Kull Ain
Published: April 2023

Phosphorus (P) is a macronutrient that often limits the productivity and growth of terrestrial ecosystems, but it is also one of the main causes of eutrophication in aquatic systems at both local and global levels. P content in soils can vary largely, but usually, only a small fraction is plant-available or in an organic form for biological utilization because it is bound in incompletely weathered mineral particles or adsorbed on mineral surfaces. Furthermore, in agricultural ecosystems, plant-available P content in topsoil is mainly controlled by fertilization and land management. To understand, model, and predict P dynamics at the landscape level, the availability of detailed observation-based P data is extremely valuable. We used more than 388,000 topsoil plant-available P samples from the period 2005 to 2021 to study spatial and temporal variability and land-use effect on soil P. We developed a mapping approach based on existing databases of soil, land-use, and fragmentary soil P measurements by land-use classes to provide spatially explicit high-resolution estimates of topsoil P at the national level. The modeled spatially detailed (1:10,000 scale) GIS dataset of topsoil P is useful for precision farming to optimize nutrient application and to increase productivity; it can also be used as input for biogeochemical models and to assess P load in inland waters and sea.
Maize (Zea mays L.) Stem Target Region Extraction and Stem Diameter Measurement Based on an Internal Gradient Algorithm in Field Conditions
Authors: Zhou Jing , Cui Mingren , Wu Yushan , Gao Yudi , Tang Yijia , Chen Zhiyi , Hou Lixin , Tian Haijuan
Published: April 2023

The target region and diameter of maize stems are important phenotyping parameters for evaluating crop vitality and estimating crop biomass. To address the issue that the target region and diameter of maize stems obtained after transplantation may not accurately reflect the true growth conditions of maize, a phenotyping monitoring technology based on an internal gradient algorithm is proposed for acquiring the target region and diameter of maize stems. Observations were conducted during the small bell stage of maize. First, color images of maize plants were captured by an Intel RealSense D435i camera. The color information in the color image was extracted by the hue saturation value (HSV) color space model. The maximum between-class variance (Otsu) algorithm was applied for image threshold segmentation to obtain the main stem of maize. Median filtering, image binarization, and morphological opening operations were then utilized to remove noise from the images. Subsequently, the morphological gradient algorithm was applied to acquire the target region of maize stems. The similarity between the three types of gradient images and the manually segmented image was evaluated by pixel ratio extraction and image quality assessment indicators. Evaluation results indicated that the internal gradient algorithm could more accurately obtain the target region of maize stems. Finally, a checkerboard was employed as a reference for measurement assistance, and the stem diameter of maize was calculated by the pinhole imaging principle. The mean absolute error of stem diameter was 1.92 mm, the mean absolute percentage error (MAPE) was 5.16%, and the root mean square error (RMSE) was 2.25 mm. The R² value was 0.79. With an R² greater than 0.7 and a MAPE within 6%, the phenotyping monitoring technology based on the internal gradient algorithm was proven to accurately measure the diameter of maize stems. The application of phenotyping monitoring technology based on the internal gradient algorithm in field conditions provides technological support for smart agriculture.
Influence of Silver Nanoparticles on Photosynthetic Pigment Content and Mineral Uptake in Pineapple Seedlings Grown In Vitro under Aluminum Stress
Authors: Tejada-Alvarado José Jesús , Meléndez-Mori Jegnes Benjamín , Ayala-Tocto Rosmery Y. , Goñas Malluri , Oliva Manuel
Published: April 2023

The presence of toxic metals such as aluminum is described as a factor that could lead to a significant decrease in crop productivity, particularly for the cultivation of acidophilic plants. In the present study, in vitro cultivation of pineapple was used as a model to evaluate the role of silver nanoparticles (0.005, 0.01, 0.025, 0.05, and 0.1 g L−1 Ag NPs) against the negative effects of aluminum (0, 100, 300, and 500 μM AlCl3). The results showed that the presence of 0.025 g L−1 Ag NPs stimulated a higher concentration of photosynthetic pigments “a”, “b”, “a + b” and carotenoids in treatments with high levels of AlCl3. The application of Ag NPs allowed better shoot formation, improved chlorophyll a/b, and total/carotenoid ratios, as well as better levels of proline biosynthesis in response to stress. The synergistic interaction of Ag NPs and AlCl3 increased the availability and assimilation of mineral elements (K, Ca, Mg, Fe, Cu, Mn, and Zn) while decreasing Al translocation. In conclusion, the implementation and validation of Ag NPs in agricultural fields would be revolutionary because they represent a novel alternative for overcoming the limitations imposed by the presence of Al.
Water Availability and Status of Wastewater Treatment and Agriculture Reuse in China: A Review
Authors: Kama Rakhwe , Song Jibin , Liu Yuan , Hamani Abdoul Kader Mounkaila , Zhao Shouqiang , Li Zhongyang
Published: April 2023

Due to climate change, 2/3 of the world’s population will face water shortage problems by 2025, while a 50% increase in food production is required in 2050 to feed nine billion people. In addition, the intensified anthropogenic activities have significantly increased water resource pollution. In this condition, wastewater reuse for crop irrigation to reduce water scarcity is currently becoming global, while it often causes soil pollution and heavy metal accumulation in agricultural areas. This situation has increased public concern over its environmental impact. Thus, an integrated framework was conducted to discuss the status of water availability in China, wastewater treatment and reuse in irrigation systems, and the potential health risks. Avenues for new research toward sustainable agriculture were discussed. We emphasize that wastewater reuse reduces the freshwater deficit and increases food productivity. However, adequate treatment should be applied before use to reduce its adverse impacts on human health risks and environmental pollution. Facilities and policies should support more accessible access to reclaimed water used in industries and urban facilities from secondary municipal wastewater treatment plants. This could be a long-term solution to eradicate water scarcity and inefficient water resources in agricultural systems.
Effect of Organic and Bio-Fertilization on Fruit Yield, Bioactive Constituents, and Estragole Content in Fennel Fruits
Authors: El-Beltagi Hossam S. , Nada Ramy S. , Mady Emad , Ashmawi Ashmawi E. , Gashash Ebtesam Abdullah , Elateeq Ahmed A. , Suliman Ahmad A. , Al-Harbi Nadi Awad , Al-Qahtani Salem Mesfir , Zarad Mostafa M. , Randhir Timothy O.
Published: April 2023

Fennel fruits (Foeniculum vulgare Mill.) represent one of the plant-based natural spices. This study aims to improve the fruit yield and essential oil (EO) quality by reducing the undesirable component, estragole, under different fertilizer treatments. The fertilizers included chemical nitrogen, phosphorus, and potassium (NPK), and also the organic additive, rabbit manure (RM). For bio-fertilization, plants were inoculated with a mixture of N-fixing bacteria, and P- and K-solubilizing bacteria with/without vesicular-arbuscular mycorrhizal fungi. The results showed that fruit and EO yield parameters, total phenolic content (TPC), total flavonoid content (TFC), and DPPH scavenging activity of fruit extracts were enhanced by fertilizer treatments in both growing seasons. NPK at 150% of the recommended dose (NPK150) and RM at 60 m3/fed (RM60) recorded the highest values of plant height, umbel number/plant, 100-fruit weight, fruit yield, chlorophyll, carbohydrates, N and P content, EO content, and yield. TPC and TFC were enhanced by using biological fertilizers. DPPH scavenging activity was higher in organically and biologically fertilized fennel. The GC-MS analyses of EO revealed higher contents of the desirable trans-anethole in the organically and chemically fertilized fennel. However, the highest proportion of estragole, the undesirable compound, was recorded for NPK150 and unfertilized plants. On the contrary, increases in the EO content and yield of fennel fruits were achieved by RM along with a reduction in estragole, enhancement in trans-anethole, and increments in other favorable compounds such as fenchone and limonene. In addition, the inhibition of estragole formation was recorded with bio-fertilizers, which also increased the trans-anethole content. Furthermore, the trans-anethole/estragole ratio was significantly higher with the application of organic and bio-fertilization. Hence, organic and bio-fertilizer resources can produce high-quality fennel fruit and EO. The reduction in the use of chemical fertilizers can help to reduce environmental pollution.
Analysis of Trace Volatile Compounds Emitted from Flat Ground and Formed Bed Anaerobic Soil Disinfestation in Strawberry Field Trials on California’s Central Coast
Authors: Prescott Kali , Kortman Stefanie , Duque Josue , Muramoto Joji , Shennan Carol , Greenstein Gloria , Haffa Arlene L. M.
Published: April 2023

Anaerobic soil disinfestation (ASD) is emerging globally as an alternative to fumigant pesticides. To investigate ASD mechanisms, we monitored microbially produced volatile organic compounds (VOCs) and other volatile gases in situ using Fourier Transform Infrared Spectroscopy. Study plots infested with Fusarium oxysporum, Macrophomina phaseolina, and/or Verticillium dahliae included: organic flat ground (fASD, 6.7 + 13.5 megagrams per hectare, Mg/ha, rice bran/broccoli) and uncovered soil treated with mustard seed meal (MSM, 3.4 Mg/ha) at one site performed in fall of 2018; formed bed (bASD, 20 Mg/harice bran), control (UTC) and fumigant (FUM) at a second field site in fall of 2019 and 2021. Here, we present VOC diversity and temporal distribution. fASD generated 39 VOCs and GHGS, including known pathogen suppressors: dimethyl sulfide, dimethyl disulfide, and n-butylamine. bASD produced 17 VOCs and greenhouses gases (GHGs), 12 of which were also detected in fASD but in greater concentrations. Plant mortality and wilt score (fASD: 3.75% ± 4.79%, 2.8 ± 0.8; MSM: 6.25% ± 12.50%, 2.7 ± 0.3; bASD: 61.27% ± 11.26%, 4.1 ± 0.5; FUM: 13.89% ± 7.17%, 2.3 ± 0.2; UTC: 76.63% ± 25.11%, 4.3 ± 1.0) were significantly lower for fASD and MSM versus bASD and UTC (p < 0.05). Only FUM was not statistically different from fASD and MSM, and was significantly lower than UTC and bASD (bASD-FUM, p < 0.05; UTC-FUM, p < 0.05). The cumulative strawberry yield from bASD-treated plots was not different from FUM or UTC (bASD: 60.3 ± 13.6; FUM: 79.4 ± 9.19; UTC: 42.9 ± 12.4 Mg/ha). FUM yield was significantly greater than UTC (p = 0.005). These results, and to a far greater extent, additional challenges faced during both bASD trials, suggest that bASD is not as effective or as feasible at maintaining overall plant health as fASD or traditional fumigants. However, differences in management practices and environmental conditions at both sites across years cannot be fully excluded from consideration and many of our observations remain qualitative in nature.
A New Idea to Improve the Test Method of Soil Aggregate Stability for Soils with a Texture Gradient
Authors: Li Jiangwen , Wei Xihao , Zhong Shouqin , Ci En , Wei Chaofu
Published: April 2023

It is of great significance to determine soil aggregate stability in predicting agricultural production conditions and soil erosion risk. However, the problem exposed in the process of evaluating soil aggregate stability cannot be ignored: Can the effects of different mechanisms on the degree of soil aggregate breakdown be distinguished by selecting ethanol and water as dispersion media? Based on this question, natural soils with a gradient in soil textures of silty loam to loamy clay were used as the test materials. Deionized water, ethanol and hexane were employed as soaking solutions to quantitatively analyze the extent to which the aggregates were dispersed in static disintegration experiments. The results suggested that the soil hydrophilicity (SH) of six soils with a texture gradient were >1 by comparing the aggregate breakdown index (ABI) of soils undergoing ethanol and hexane dispersion. This indicated that the hydrophilic group (-OH) contained in ethanol interacted with the hydrophilic surfaces of the soil particles. Therefore, the soil hydrophilicity (hydration) should be determined by comparing the ABI values undergoing hexane and water dispersion. From silty loam to loamy clay, the average contribution of hydration to aggregate fragmentation decreases, and the process of aggregate breakdown with different textures is characterized by size selectivity. When the soil aggregates were fragmented into 2–0.25 mm aggregate fractions, for silty loam and sandy clay loam, 0.002–0.02 mm and <0.002 mm particles were preferentially moved; for clay loam and loamy clay, >0.002 mm particles were preferentially moved. When the soil aggregates were fragmented into <0.25 mm aggregate fractions for soils with different textures, the 0.002–0.02 mm and <0.002 mm particles all exhibited preferential migration characteristics. This work provides an idea for improving the methods of aggregate stability measurements in the future.
Biological and Molecular Characterization of Clover Yellow Vein Virus Infecting Trifolium repens in China
Authors: Li Zhengnan , Xu Lei , Sun Pingping , Zhu Mo , Zhang Lei , Zhang Bin , Song Shuang
Published: April 2023

White clover (Trifolium repens L.) is an important perennial legume forage and ornamental plant, and is widely distributed and cultivated in the world. Recently, white clover plants showing symptoms of leaf mosaic and redding were observed in Hohhot, Inner Mongolia of China. In this work, flexuous filamentous viral particles of about 700 × 13 nm in size were observed in the symptomatic leaf samples. The infection of clover yellow vein virus (ClYVV) was confirmed by small RNA sequencing and RT-PCR validation. Mechanical inoculation assays showed that this ClYVV isolate (ClYVV-IM) can infect a range of herbaceous species, including Nicotiana benthamiana, N. occidentalis, Chenopodium quinoa, C. amaranticolor, Vicia faba, Vigna unguiculata, and Solanum lycopersicum, causing various symptoms. The complete genome sequence of ClYVV-IM consists of 9565 nt and shared sequence identities, ranging from 83.05% to 96.30%, with those of the other ClYVV isolates published in GenBank. Phylogenetic analyses based on the polyprotein nucleotide and amino acid sequences clustered 15 ClYVV isolates into two groups and ClYVV-IM located in Group I. Two potential recombination events located at 914–2970 nt and 5153–5694 nt were detected in the genome of ClYVV-IM. To our knowledge, this is the first report of occurrence and complete genome of ClYVV infecting white clover in China.
Effect of Transplanting Date on Agronomic and Grain Quality Traits Using Early-Maturing Rice Varieties
Authors: Yun Yeotae , Kim Gyucheol , Cho Giwon , Yun Tugsang
Published: April 2023

This study aimed to investigate how transplanting date affects the agronomic and grain quality traits of two early-maturing rice varieties. The experiment was conducted in the rice research field of Chungnam Agricultural Research and Extension Services in South Korea and rice materials were transplanted at intervals of approximately 15 days from 16 April to 16 July in 2019 and 2020. Results showed that agronomic and grain quality traits varied according to the transplanting date and earlier transplanting resulted in a longer period of days from transplanting to heading (DTH). The spikelet number m−2 was highly correlated with the milled rice yield (r = 0.963 ** for Jinbuol, r = 0.909 ** for Yeoreumi) and it significantly decreased as the transplanting date was delayed, which was leading to lower yield. The mean temperature during the grain filling stage had a negative correlation with head rice rate (r2 = 0.825 ** for Jinbuol, r2 = 0.803 ** for Yeoreumi) and the number of days from transplanting to heading showed negative correlation with protein content (r2 = 0.777 ** for Jinbuol, r2 = 0.833 ** for Yeoreumi). Therefore, increasing the number of days from transplanting to heading date can lead to higher milled rice yield and lower protein content and avoiding heading dates on 17 July can improve the appearance traits. As a result, it is suggested that early transplanting is advantageous to increase the milled rice yield and grain quality of early-maturing rice.
Impacts of Saline Water Irrigation on Soil Respiration from Cotton Fields in the North China Plain
Authors: Zhou Shuang , Gao Yang , Zhang Junpeng , Pang Jie , Hamani Abdoul Kader Mounkaila , Xu Chunping , Dang Hongkai , Cao Caiyun , Wang Guangshuai , Sun Jingsheng
Published: April 2023

Saline water irrigation has been widely used for crop production where agriculture is short of freshwater. However, information about the response of soil respiration to saline water irrigation is limited. To identify the effect of saline water irrigation on soil respiration, the experiment based on long-term saline water irrigation cotton fields (since 2006) was conducted in the Heilonggang area in 2021. Five salinity levels in irrigation water were tested (3.4 [S1], 7.1 [S2], 10.6 [S3], 14.1 [S4], and 17.7 dS m−1 [S5]), and deep ground water (1.3 dS m−1) was used as the control (CK). After 15 years of saline water irrigation, we monitored soil physicochemical properties and soil respiration. In addition, we developed a structural equation model of the relationship between them. The results demonstrated that saline water irrigation significantly reduced soil water-stable aggregate content and porosity by 4.42–45.33% and 6.52–14.10%, respectively, and attenuated soil cellulase, α-glucosidase, and alkaline phosphatase activity. Soil respiration under saline water irrigation was significantly reduced by 5.28–33.08%. Moreover, saline water irrigation with salinity below 10.6 dS m−1 had no significant effect on cotton yield. Moreover, soil salinity (62%), water-stable aggregate content (46%), and soil porosity (25%) had significant effects on soil respiration, and soil porosity had a significant positive effect on soil alkaline phosphatase activity according to the structural equation model. Overall, saline irrigation with salinity below 10.6 dS m−1 can alleviate water shortages and reduce soil carbon emissions without affecting cotton yield in the study area.
Role of Exogenous Nitric Oxide in Protecting Plants against Abiotic Stresses
Authors: Pandey Anamika , Khan Mohd. Kamran , Hamurcu Mehmet , Athar Tabinda , Yerlikaya Bayram Ali , Yerlikaya Seher , Kavas Musa , Rustagi Anjana , Zargar Sajad Majeed , Sofi Parvaze A. , Chaudhry Bharti , Topal Ali , Gezgin Sait
Published: April 2023

The continuously changing environment has led to devastating effects on the normal growth and development of plants. This necessitates the understanding of different components that can be involved in alleviating these effects. In the last two decades, nitric oxide (NO) has been largely focused on as a molecule whose endogenous production and exogenous supply lead to several molecular and physiological changes in plants under stressed conditions. Although its role as a signaling molecule in endogenous production has been largely discussed, its function in dealing with contemporary abiotic stress conditions on exogenous supply remains comparatively less explored. There is growing evidence that NO plays a critical role in many physiological processes; however, there is debate about the exact mechanism(s) through which NO lessens abiotic stress on external supply. In this review, we discuss the studies that were focused on observing the effect of exogenous NO on different abiotic stresses including heavy metal stress, element deficiency or toxicity stress, salt stress, drought stress, ultraviolet radiation stress, waterlogging stress, and chilling stress. Though the positive effects of endogenous NO have been discussed in brief in different sections, the focus of the review is to discuss the effects of exogenous NO on plant grown under abiotic stresses. Deciphering the underlying mechanism of exogenous NO treatment may open up new ideas that can suggest the successful application of NO in agricultural regions to reduce the damaging influences of different abiotic stresses.
Modified Cassava Starches’ Identification through Mid-Infrared Spectroscopy and Exploratory Analysis
Authors: de Macêdo Isaac Yves Lopes , Dupuy Nathalie , Brito Vitor H. dos S. , Gil Eric de Souza , Silva Giovanna N. de M. e , Moreno Emily K. G. , Demiate Ivo M. , Cereda Marney P.
Published: April 2023

Different starch properties may cause alterations in the foodstuff’s external appearance. However, modification processes in starches are usually secretive. The use of chemically modified starches is regulated by international standards, which makes it important to identify its presence and type. Mid-infrared spectroscopy (MIR)-modified starches’ identification can be hindered by the presence of excess glucose. This research investigates types of modification in commercial starches and in approaches that circumvent MIR’s limitations with exploratory analysis. It also considers that enzymatic hydrolysis (α-amylase and amyloglucosidase) can highlight the points of modification in the structure, which can be detected with infrared assays. To determine if sour cassava starch (FCS) is modified and check its type, six samples were selected: a native one, three of the most common cassava modified starches (etherified, esterified, and FCS), and two laboratory processed samples (Acid, Oxidized). The results showed that the sour cassava starch showed similarities with a commercial ester and an oxidized cassava starch, which may be due to the formation of a graft, corresponding to what the literature has already reported for corn starch treated with lactic acid and gamma radiation.
Sodium Salicylate as Feed Additive in Broilers: Absence of Toxicopathological Findings
Authors: Di Gregorio Mayra Carraro , de Almeida Elaine Renata Motta , Momo Claudia , da Silva Araújo Cristiane Soares , Hueza Isis Machado , Andréo-Filho Newton , Raspantini Leonila Ester Reinert , Gotardo André Tadeu , Górniak Silvana Lima
Published: April 2023

Antimicrobial growth promoters (AGPs) in animal production have been related to the increase in multidrug-resistant bacteria. The AGP ban in many countries has highlighted the growing need for alternatives for feed additives. Considering the non-antibiotic anti-inflammatory theory of AGPs, chicks received three different doses of sodium salicylate (SS) in feed (10, 30, 90 mg/kg), basal diet (BD) was used as a negative control, and zinc bacitracin (ZB) was used as a positive control. Chicks were individually housed to increase the accuracy of the dose of SS ingested. Performance parameters and footpad dermatitis were evaluated weekly, while haematology, serum biochemistry, histopathology, and tibial dyschondroplasia were determined on Days 21 and 42. A linear dose-dependent decrease in haemoglobin concentration was observed, but the values were within the normal reference range. Among all the other evaluated parameters, no relevant differences between treatments were observed; however, not even the AGP group performed better than the control group. It is possible that the conditions in which the birds were raised were not stressful enough to allow for anti-inflammatories to demonstrate their beneficial effects on performance. Studies should be conducted where the animals are exposed to commercial conditions, as the presence of natural stressors could allow a better evaluation of the efficacy of the anti-inflammatory agent as a growth promoter.
Current Antimicrobial Use in Horses Undergoing Exploratory Celiotomy: A Survey of Board-Certified Equine Specialists
Authors: Rockow Meagan , Griffenhagen Gregg , Landolt Gabriele , Hendrickson Dean , Pezzanite Lynn
Published: April 2023

In the past decade, there has been a considerable increase in the recognition of antimicrobial resistance in equine practice. The objective of this study was to survey the current clinical use of antimicrobials for a commonly performed surgical procedure (exploratory celiotomy) with the goal of understanding how recent literature and changes in microbial resistance patterns may have impacted antimicrobial selection practices. An electronic survey was distributed to veterinary professionals within the American College of Veterinary Internal Medicine (ACVIM) and the American College of Veterinary Surgery (ACVS). A total of 113 completed surveys were returned. Practitioners reported antimicrobials were most frequently given 30–60 min preoperatively (63.1%). Two antimicrobial classes were typically administered (95.5%), with gentamicin (98.2%) and potassium penicillin (74.3%) being the most common. Antimicrobials were typically not re-dosed intraoperatively (78.6%). Factors that affected overall treatment length postoperatively included resection (81.4%), bloodwork (75.2%), enterotomy (74.3%), fever (85.0%), incisional complications (76.1%), and thrombophlebitis (67.3%). The most common duration of antimicrobial use was 1–3 d for non-strangulating lesions (54.4% of cases) and inflammatory conditions such as enteritis or peritonitis (50.4%), and 3–5 d for strangulating lesions (63.7%). Peri-incisional and intra-abdominal antimicrobials were used by 24.8% and 11.5% of respondents, respectively. In summary, antimicrobial usage patterns were highly variable among practitioners and, at times, not concordant with current literature.
The Agreement between Radiography and Fluoroscopy as Diagnostic Tools for Tracheal Collapse in Dogs
Authors: Yangwanitset Wasutorn , Huaijantug Somkiat , Tansakul Mookmanee , Sakcamduang Walasinee
Published: April 2023

Tracheal collapse is a common disease in small, middle-aged dogs, and imaging tools are essential for its diagnosis. Radiography and fluoroscopy are the main diagnostic modalities used, but their agreement in different regions is not well documented. In this study, the agreement between thoracic radiography and fluoroscopy in tracheal collapse was investigated in 29 dogs. The results showed that radiography detected a lower degree of collapsing trachea compared to fluoroscopy at the carina region (p < 0.001). However, there was no significant difference observed between the degree of collapsing trachea detected by radiography and fluoroscopy at the cervical, thoracic inlet, and intra-thoracic regions (p = 0.780, 0.537, and 0.213, respectively). The kappa statistic indicated a slight agreement at the cervical region at a 16.4% cut-off (κ = 0.20), while the other regions showed a non-agreement. In conclusion, although radiography is useful for screening, fluoroscopy was able to detect the degree of the collapsing trachea greater than radiography in the carina region. Additionally, if a collapse in the cervical region is detected by radiography, it is prone to have a positive relationship with fluoroscopy as well.
Initial Characterization of 3D Culture of Yolk Sac Tissue
Authors: Pereira Vitória Mattos , Pinto Priscila Avelino Ferreira , Motta Lina Castelo Branco , Almeida Matheus F. , de Andrade André Furugen Cesar , Pavaneli Ana Paula Pinoti , Ambrósio Carlos Eduardo
Published: April 2023

The role of the yolk sac (YS) in miscarriage is not yet clear, largely due to ethical reasons that make in vivo studies difficult to conduct. However, 3D cultures could provide a solution to this problem by enabling cells to be arranged in a way that more closely mimics the structure of the YS as it exists in vivo. In this study, three domestic species (porcine, canine, and bovine) were chosen as models to standardize 3D culture techniques for the YS. Two techniques of 3D culture were chosen: the Matrigel® and Hanging-Drop techniques, and the 2D culture technique was used as a standardized method. The formed structures were initially characterized using scanning electron microscopy (SEM), immunohistochemistry (IHC), and quantitative real-time PCR (RT-qPCR). In general, the 3D culture samples showed better organization of the YS cells compared to 2D cultures. The formed structures from both 3D methods assemble the mesothelial layer of YS tissue. Regarding the IHC assay, all in vitro models were able to express zinc and cholesterol transport markers, although only 3D culture techniques were able to generate structures with different markers pattern, indicating a cell differentiation process when compared to 2D cultures. Regarding mRNA expression, the 3D models had a greater gene expression pattern on the Hemoglobin subunit zeta-like (HBZ) gene related to the YS tissue, although no significant expression was found in Alpha-fetoprotein (AFP), indicating a lack of endodermal differentiation in our 3D model. With the initial technique and characterization established, the next step is to maintain the cultures and characterize the diversity of cell populations, stemness, functions, and genetic stability of each 3D in vitro model.
Spray-Dried Plasma Promotes Broiler Chick Growth by Enhancing Immune Surveillance
Authors: Blue Candice E. C. , Jababu Yasin , Ibrahim Salam A. , Minor Radiah C. , Williams Leonard L. , Adetunji Adedeji O. , Ali Rizwana , Young Lea S. , Fasina Yewande O.
Published: April 2023

Spray-dried plasma (SDP) contain a variety of functional proteins that play an immunomodulatory role. To evaluate the potential of SDP to stimulate the immune system, day-old Ross 708 male broiler chicks (200) were allocated randomly to five dietary treatments. Treatment 1 (CX) comprised chicks fed basal unmedicated corn–soybean meal (SBM) without the addition of SDP. Treatment 2 (MX) includes chicks fed unmedicated corn–SBM basal containing Bacitracin methylene disalicylate (BMD) at 0.055 g/kg diet. Treatments 3 (SDP1), 4 (SDP2), and 5 (SDP3) contained chicks given unmedicated corn–SBM basal, into which SDP was included at 10, 20, and 30 g/kg diet, respectively. On d 7, 14, and 21, chicks’ body weight and FCR were calculated. Additionally, leucocyte counts, oxidative status, and IgY concentrations were determined in blood. On d 23, fecal populations of selected indicator bacteria species were determined. Results showed that FCR for SP3 was superior (p < 0.05) to other treatments. Likewise, heterophil numbers decreased in MX and SDP treatments compared to CX. Circulating IgY concentration was higher for SDP dietary treatments (p < 0.05) compared to MX. In conclusion, dietary SDP at 30 g/kg enhanced immune surveillance by increasing circulating IgY levels, maintaining a normal oxidative state, and increasing gut Bifidobacteria, thereby improving chick growth performance.
How Pendular Is Human Brachiation? When Form Does Not Follow Function
Authors: Young Melody W. , Virga James Q. , Kantounis Stratos J. , Lynch Samantha K. , Chernik Noah D. , Gustafson Jon A. , Cannata Matthew J. , Flaim Nicholas D. , Granatosky Michael C.
Published: April 2023

Brachiation is a form of suspensory locomotion observed only in Primates. The non-human hominoids (e.g., gibbons, orangutans, chimpanzees, and gorillas) are considered specialized brachiators, yet peculiar among the living apes are anatomically modern humans (Homo sapiens), who have forgone this locomotor mode in favor of bipedal striding. Humans can, however, brachiate and seem to have retained the locomotor capabilities of their arboreal ancestors. However, the mechanics of human brachiation have not been quantified. In this study, we evaluate how closely human brachiation conforms to the expectations of simple pendular motion using triaxial accelerometry and high-speed videography. These data are compared to specialized brachiating non-human primates. We found that humans have lower energy recovery than siamangs (Symphalangus syndactylus) during brachiation and have shorter observed pendular periods than expected compared to other primates. We demonstrate that relatively long forelimb length and high grip forces, a proxy for global forelimb force-generating potential, act as the main driving factors to reduce energetic costs through effective pendular recovery. These data are the first to assess the strategies humans adopt to perform a behavior they are not anatomically specialized to execute and places them within a comparative framework amongst other brachiating primates. We show that although humans demonstrate behavioral flexibility during brachiation (e.g., differing mediolateral and vertical center of mass positional movement patterns), anatomical features are the primary driver of variation in brachiation performance.
Effects of Curcumin on Growth Performance, Ruminal Fermentation, Rumen Microbial Protein Synthesis, and Serum Antioxidant Capacity in Housed Growing Lambs
Authors: Tian Guangyuan , Zhang Xuanzi , Hao Xiaoyan , Zhang Jianxin
Published: April 2023

This experiment was conducted to investigate growth performance, ruminal fermentation, rumen microbial protein synthesis, and serum antioxidant capacity with different doses of curcumin (CUR) included in the diet of housed growing lambs. Forty-eight four-month-old Dorper × Thin-tailed Han F1 crossbred male lambs (body weight = 20.89 ± 1.15 kg, age = 120 ± 10 days; mean ± SD) were randomly divided into four groups for a single-factor, completely randomized experiment. Treatments comprised the following: the basal diet supplemented with 0 (Control), 300 mg/kg (300 CUR), 600 mg/kg (600 CUR), or 900 mg/kg (900 CUR) CUR, respectively. The results showed that dietary CUR increased average daily gain (ADG), and the 300 CUR group evidenced the highest value. There were no significant effects on dry matter intake (DMI) and DMI/ADG. Lambs in the 300 CUR group showed higher totals of volatile fatty acids (VFA) and acetate than other groups, while decreased valerate was observed with supplementary CUR. The ruminal pH and ammonia N (NH3-N) concentration decreased with increasing CUR, with the greatest effect in the 300 CUR group. The quadratic effects were found in pectinase, carboxymethyl cellulose, and protease, with the greatest value in the 300 CUR group. The microbial populations of total bacteria and Ruminococcus albus also responded quadratically, and the methanogens, protozoan, and Fibrobacter succinogenes populations decreased linearly with increasing CUR. Lambs receiving additional CUR showed increased Prevotella ruminicola population. Microbial protein (MCP) synthesis was promoted by supplementary CUR. As supplementation with CUR increased, the serum activity of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) was enhanced, with the greatest value in the 300 CUR group. In conclusion, dietary CUR improved ruminal fermentation, promoted rumen microbial protein (MCP) synthesis, and enhanced serum antioxidant activity, as well as promoting growth performance in housed growing lambs.
LAD-RCNN: A Powerful Tool for Livestock Face Detection and Normalization
Authors: Sun Ling , Liu Guiqiong , Yang Huiguo , Jiang Xunping , Liu Junrui , Wang Xu , Yang Han , Yang Shiping
Published: April 2023

With the demand for standardized large-scale livestock farming and the development of artificial intelligence technology, a lot of research in the area of animal face detection and face identification was conducted. However, there are no specialized studies on livestock face normalization, which may significantly reduce the performance of face identification. The keypoint detection technology, which has been widely applied in human face normalization, is not suitable for animal face normalization due to the arbitrary directions of animal face images captured from uncooperative animals. It is necessary to develop a livestock face normalization method that can handle arbitrary face directions. In this study, a lightweight angle detection and region-based convolutional network (LAD-RCNN) was developed, which contains a new rotation angle coding method that can detect the rotation angle and the location of the animal’s face in one stage. LAD-RCNN also includes a series of image enhancement methods to improve its performance. LAD-RCNN has been evaluated on multiple datasets, including a goat dataset and infrared images of goats. Evaluation results show that the average precision of face detection was more than 97%, and the deviations between the detected rotation angle and the ground-truth rotation angle were less than 6.42° on all the test datasets. LAD-RCNN runs very fast and only takes 13.7 ms to process a picture on a single RTX 2080Ti GPU. This shows that LAD-RCNN has an excellent performance in livestock face recognition and direction detection, and therefore it is very suitable for livestock face detection and normalization.
Comparison of the Effects of Yucca saponin, Yucca schidigera, and Quillaja saponaria on Growth Performance, Immunity, Antioxidant Capability, and Intestinal Flora in Broilers
Authors: Dai Zhenglie , Wang Huixian , Liu Jinsong , Zhang Haoran , Li Qing , Yu Xiaorong , Zhang Ruiqiang , Yang Caimei
Published: April 2023

The purpose of this study is to investigate the effects of Yucca saponin (YSa), Yucca schidigera (YS), and Quillaja Saponaria (QS) on growth performance, nitrogen metabolism, immune ability, antioxidant capability, and intestinal flora of yellow-feather broilers. This study randomly divided a total of 480 1-day yellow-feather broilers into 4 treatment groups. Factors in the 4 groups included CON group (basic diet), YSa group (basic diet mixed with 500 mg/kg YSa), YS group (basic diet mixed with 500 mg/kg YS), and QS group (basic diet mixed with 500 mg/kg QS). Throughout the 56-day study period, YSa, YS, and QS groups had higher average daily gain in broilers than the CON group (p < 0.01). The YS group had a lower feed gain ratio (F: G) in broilers than the CON group (p < 0.05). YSa, YS, and QS showed increased serum immunoglobin A (IgA), immunoglobin Y (IgY), immunoglobin M (IgM), and total antioxidant capacity (T-AOC) levels; enhanced acetic acid, butyric acid, and valeric acid levels of cecal content; and reduced contents of ammonia nitrogen, urea nitrogen, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and malondialdehyde (MDA) in serum in broilers (p < 0.05). The relative abundance of Lachnoclostridium in the QS group was decreased compared with that in the CON group (p < 0.05). Higher IgA and IgY sera contents were observed in the YS group compared to the YSa and QS groups (p < 0.05). In contrast with the QS group, the serum IL-6 concentration of the YS group was reduced (p < 0.05). In conclusion, YSa, YS, and QS promoted growth performance, nitrogen metabolism, immunity, antioxidant capability, and intestinal flora in broilers. Through the comparison of YSa, YS, and QS, it was found that YS is more suitable as a feed additive to ameliorate the healthy growth of broilers.