bluuebunny/crossref_metadata_2025 huggingface dataset:
The Clamping of End-Tidal Carbon Dioxide Does Not Influence Cognitive Function Performance During Moderate Hyperthermia With or Without Skin Temperature Manipulation
Authors: Schultz Martins Ricardo , Wallace Phillip J. , Steele Scott W. , Scott Jake S. , Taber Michael J. , Hartley Geoffrey L. , Cheung Stephen S.
Published: December 2021

Increases in body temperature from heat stress (i.e., hyperthermia) generally impairs cognitive function across a range of domains and complexities, but the relative contribution from skin versus core temperature changes remains unclear. Hyperthermia also elicits a hyperventilatory response that decreases the partial pressure of end-tidal carbon dioxide (PetCO2) and subsequently cerebral blood flow that may influence cognitive function. We studied the role of skin and core temperature along with PetCO2 on cognitive function across a range of domains. Eleven males completed a randomized, single-blinded protocol consisting of poikilocapnia (POIKI, no PetCO2 control) or isocapnia (ISO, PetCO2 maintained at baseline levels) during passive heating using a water-perfused suit (water temperature ~ 49°C) while middle cerebral artery velocity (MCAv) was measured continuously as an index of cerebral blood flow. Cognitive testing was completed at baseline, neutral core-hot skin (37.0 ± 0.2°C-37.4 ± 0.3°C), hot core-hot skin (38.6 ± 0.3°C-38.7 ± 0.2°C), and hot core-cooled skin (38.5 ± 0.3°C-34.7 ± 0.6°C). The cognitive test battery consisted of a detection task (psychomotor processing), 2-back task (working memory), set-shifting and Groton Maze Learning Task (executive function). At hot core-hot skin, poikilocapnia led to significant (both p < 0.05) decreases in PetCO2 (∆−21%) and MCAv (∆−26%) from baseline, while isocapnia clamped PetCO2 (∆ + 4% from baseline) leading to a significantly (p = 0.023) higher MCAv (∆−18% from baseline) compared to poikilocapnia. There were no significant differences in errors made on any task (all p > 0.05) irrespective of skin temperature or PetCO2 manipulation. We conclude that neither skin temperature nor PetCO2 maintenance significantly alter cognitive function during passive hyperthermia.
Attention Deficit/Hyperactivity Disorder Symptoms Impair Adaptive and Social Function in Children With Autism Spectrum Disorder
Authors: Liu Yu , Wang Luxi , Xie Shu , Pan Shixu , Zhao Jingyi , Zou Mingyang , Sun Caihong
Published: December 2021

Background: Autism spectrum disorder (ASD) often co-exists with attention deficit/hyperactivity disorder (ADHD), which may aggravate functional impairment. However, it is unclear how comorbid ADHD symptoms influence the adaptive behavior and social interaction deficits of children with ASD.

Methods: The study enrolled 340 children (ranging from 2 to 14 years) with ASD, with comorbid ASD and ADHD, or with typical development (TD). A psychological evaluation involving adaptive behavior and social function was conducted using the Vineland Adaptive Behavior Scale, Second Edition (VABS-II) and the Social Responsiveness Scale (SRS).

Results: There was a high prevalence of ADHD symptoms (46.6%) in children with ASD, and children with ASD + ADHD presented the worse profile of ASD symptoms. The ASD + ADHD group had higher scores on VABS and lower scores on SRS in comparison with the ASD alone group and TD group. The regression analysis revealed that ASD symptoms and ADHD symptoms were significantly associated with greater impairments in adaptive behavior and social function. The ADHD symptoms were responsible for an additional 0.8% of the variance in adaptive behavior, and 9.5% of the variance in social function.

Conclusions: More severe ASD symptoms and greater impairment in adaptive function and social ability were found in children with ASD and comorbid ADHD, highlighting the need to identify ADHD comorbidities early on in children with ASD and to reduce their negative impact on functioning.
Examining Differences in Fear Learning in Patients With Obsessive-Compulsive Disorder With Pupillometry, Startle Electromyography and Skin Conductance Responses
Authors: Pöhlchen Dorothee , Priouret Marthe , Kraft Miriam S. , Binder Florian P. , Gürsel Deniz A. , Berberich Götz ,   BeCOME working group, Koch Kathrin , Spoormaker Victor I.
Published: December 2021

Obsessive-compulsive disorder (OCD) is characterized by recurrent, persistent thoughts and repetitive behaviors causing stress and anxiety. In the associative learning model of OCD, mechanisms of fear extinction are supposed to partly underlie symptom development, maintenance and treatment of OCD, proposing that OCD patients suffer from rigid memory associations and inhibitory learning deficits. To test these assumptions, previous studies have used skin conductance and subjective ratings as readouts in fear conditioning paradigms, finding impaired fear extinction learning, impaired fear extinction recall or no differences between individuals with OCD and healthy controls. Against this heterogeneous background, we tested fear acquisition and extinction in 37 OCD patients and 56 healthy controls, employing skin conductance as well as pupillometry and startle electromyography. Extinction recall was also included in a subsample. We did not observe differences between groups in any of the task phases, except a trend toward higher startle amplitudes during extinction for OCD. Overall, sensitive readouts such as pupillometry and startle responses did not provide evidence for moderate-to-large inhibitory learning deficits using classical fear conditioning, challenging the assumption of generically impaired extinction learning and memory in OCD.
The Effects of Bipolar Disorder Risk on a Mobile Phone Keystroke Dynamics Based Biomarker of Brain Age
Authors: Zulueta John , Demos Alexander Pantelis , Vesel Claudia , Ross Mindy , Piscitello Andrea , Hussain Faraz , Langenecker Scott A. , McInnis Melvin , Nelson Peter , Ryan Kelly , Leow Alex , Ajilore Olusola
Published: December 2021

Background: Research by our group and others have demonstrated the feasibility of using mobile phone derived metadata to model mood and cognition. Given the effects of age and mood on cognitive performance, it was hypothesized that using such data a model could be built to predict chronological age and that differences between predicted age and actual age could be a marker of pathology.

Methods: These data were collected via the ongoing BiAffect study. Participants complete the Mood Disorders Questionnaire (MDQ), a screening questionnaire for bipolar disorder, and self-reported their birth year. Data were split into training and validation sets. Features derived from the smartphone kinematics were used to train random forest regression models to predict age. Prediction errors were compared between participants screening positive and negative on the MDQ.

Results: Three hundred forty-four participants had analyzable data of which 227 had positive screens for bipolar disorder and 117 had negative screens. The absolute prediction error tended to be lower for participants with positive screens (median 4.50 years) than those with negative screens (median 7.92 years) (W = 508, p = 0.0049). The raw prediction error tended to be lower for participants with negative screens (median = −5.95 years) than those with positive screens (median = 0.55 years) (W = 1,037, p= 0.037).

Conclusions: The tendency to underestimate the chronological age of participants screening negative for bipolar disorder compared to those screening positive is consistent with the finding that bipolar disorder may be associated with brain changes that could reflect pathological aging. This interesting result could also reflect that those who screen negative for bipolar disorder and who engaged in the study were more likely to have higher premorbid functioning. This work demonstrates that age-related changes may be detected via a passive smartphone kinematics based digital biomarker.
Novel Probable Glance at Inflammatory Scenario Development in Autistic Pathology
Authors: Harutyunyan Aida A. , Harutyunyan Hayk A. , Yenkoyan Konstantin B.
Published: December 2021

Autism Spectrum Disorder (ASD) is characterized by persistent deficits in social communication and restricted-repetitive patterns of behavior, interests, or activities. ASD is generally associated with chronic inflammatory states, which are linked to immune system dysfunction and/or hyperactivation. The latter might be considered as one of the factors damaging neuronal cells. Several cell types trigger and sustain such neuroinflammation. In this study, we traced different markers of immune system activation on both cellular (immune cell phenotypes) and mediatory levels (production of cytokines) alongside adverse hematology and biochemistry screening in a group of autistic children. In addition, we analyzed the main metabolic pathways potentially involved in ASD development: energy (citric acid cycle components), porphyrin, and neurotransmitter metabolism. Several ASD etiological factors, like heavy metal intoxication, and risk factors—genetic polymorphisms of the relevant neurotransmitters and vitamin D receptors—were also analyzed. Finally, broad linear regression analysis allowed us to elucidate the possible scenario that led to the development of chronic inflammation in ASD patients. Obtained data showed elevated levels of urinary cis-aconitate, isocitrate, alfa-ketoglutarate, and HMG. There were no changes in levels of metabolites of monoamine neurotransmitters, however, the liver-specific tryptophan kinurenine pathway metabolites showed increased levels of quinolinate (QUIN) and picolinate, whereas the level of kynurenate remained unchanged. Abovementioned data demonstrate the infringement in energy metabolism. We found elevated levels of lead in red blood cells, as well as altered porphyrin metabolism, which support the etiological role of heavy metal intoxication in ASD. Lead intoxication, the effect of which is intensified by a mutation of the VDR-Taq and MAO-A, leads to quinolinic acid increase, resulting in energy metabolism depletion and mitochondrial dysfunction. Moreover, our data backing the CD4+CD3+ T-cell dependence of mitochondrial dysfunction development in ASD patients reported in our previous study leads us to the conclusion that redox-immune cross-talk is considered a main functional cell damaging factor in ASD patients.
Consequences of a Diagnostic Label: A Systematic Scoping Review and Thematic Framework
Authors: Sims Rebecca , Michaleff Zoe A. , Glasziou Paul , Thomas Rae
Published: December 2021

Objectives: To develop a thematic framework for the range of consequences arising from a diagnostic label from an individual, family/caregiver, healthcare professional, and community perspective.

Design: Systematic scoping review of qualitative studies.

Search Strategy: We searched PubMed, Embase, PsycINFO, Cochrane, and CINAHL for primary studies and syntheses of primary studies that explore the consequences of labelling non-cancer diagnoses. Reference lists of included studies were screened, and forward citation searches undertaken.

Study Selection: We included peer reviewed publications describing the perceived consequences for individuals labelled with a non-cancer diagnostic label from four perspectives: that of the individual, their family/caregiver, healthcare professional and/or community members. We excluded studies using hypothetical scenarios.

Data Extraction and Synthesis: Data extraction used a three-staged process: one third was used to develop a preliminary framework, the next third for framework validation, and the final third coded if thematic saturation was not achieved. Author themes and supporting quotes were extracted, and analysed from the perspective of individual, family/caregiver, healthcare professional, or community member.

Results: After deduplication, searches identified 7,379 unique articles. Following screening, 146 articles, consisting of 128 primary studies and 18 reviews, were included. The developed framework consisted of five overarching themes relevant to the four perspectives: psychosocial impact (e.g., positive/negative psychological impact, social- and self-identity, stigma), support (e.g., increased, decreased, relationship changes, professional interactions), future planning (e.g., action and uncertainty), behaviour (e.g., beneficial or detrimental modifications), and treatment expectations (e.g., positive/negative experiences). Perspectives of individuals were most frequently reported.

Conclusions: This review developed and validated a framework of five domains of consequences following diagnostic labelling. Further research is required to test the external validity and acceptability of the framework for individuals and their family/caregiver, healthcare professionals, and community.
Assessing the Quality of Care at Primary Health Care Level in Two Pilot Regions of Albania
Authors: Saric Jasmina , Kiefer Sabine , Peshkatari Altina , Wyss Kaspar
Published: December 2021

The quality of care (QoC) of primary health care (PHC) services in Albania faces challenges on multiple levels including governance, access, infrastructure and health care workers. In addition, there is a lack of trust in the latter. The Health for All Project (HAP) funded by the Swiss Agency for Development and Cooperation therefore aimed at enhancing the population's health by improving PHC services and implementing health promotion activities following a multi-strategic health system strengthening approach. The objective of this article is to compare QoC before and after the 4 years of project implementation. A cross-sectional study was implemented at 38 PHC facilities in urban and rural locations in the Diber and Fier regions of Albania in 2015 and in 2018. A survey measured the infrastructure of the different facilities, provider–patient interactions through clinical observation and patient satisfaction. During clinical observations, special attention was given to diabetes and hypertensive patients. Infrastructure scores improved from base- to endline with significant changes seen on national level and for rural facilities (p < 0.01). Facility infrastructure and overall cleanliness, hygiene and basic/essential medical equipment and supplies improved at endline, while for public accountability/transparency and guidelines and materials no significant change was observed. The overall clinical observation score increased at endline overall, in both areas and in rural and urban setting. However, infection prevention and control procedures and diabetes treatment still experienced relatively low levels of performance at endline. Patient satisfaction on PHC services is generally high and higher yet at endline. The changes observed in the 38 PHC facilities in two regions in Albania between 2015 and 2018 were overall positive with improvements seen at all three levels assessed, e.g., infrastructure, service provision and patient satisfaction. However, to gain overall improvements in the QoC and move toward a more efficient and sustainable health system requires continuous investments in infrastructure alongside interventions at the provider and user level.
Multi-Frequency Radar Micro-Doppler Based Classification of Micro-Drone Payload Weight
Authors: Dhulashia Dilan , Peters Nial , Horne Colin , Beasley Piers , Ritchie Matthew
Published: December 2021

The use of drones for recreational, commercial and military purposes has seen a rapid increase in recent years. The ability of counter-drone detection systems to sense whether a drone is carrying a payload is of strategic importance as this can help determine the potential threat level posed by a detected drone. This paper presents the use of micro-Doppler signatures collected using radar systems operating at three different frequency bands for the classification of carried payload of two different micro-drones performing two different motions. Use of a KNN classifier with six features extracted from micro-Doppler signatures enabled mean payload classification accuracies of 80.95, 72.50 and 86.05%, for data collected at S-band, C-band and W-band, respectively, when the drone type and motion type are unknown. The impact on classification performance of different amounts of situational information is also evaluated in this paper.
London Calls? Discrimination of European Job Seekers in the Aftermath of the Brexit Referendum
Authors: Di Stasio Valentina , Heath Anthony Francis
Published: December 2021

The central question in this article is whether there was greater discrimination against European applicants in the labor market in those English regions where public opinion was more strongly in favor of Brexit. Using a field experiment conducted immediately after the Brexit Referendum, we provide causal evidence that applicants with EU backgrounds faced discrimination when applying for jobs in England. On average, applicants from EU12 countries and applicants from Eastern European member states were both less likely to receive a callback from employers than were white British applicants. Furthermore, in British regions where support for Brexit was stronger, employers were more likely to discriminate against EU12 applicants. This finding, though, is driven by the more favorable treatment reserved to EU12 applicants applying for jobs in the Greater London area. Eastern Europeans, on the other hand, did not benefit from this ‘London advantage’. Administrative and legal uncertainties over the settlement status of EU nationals cannot explain these findings, as European applicants, both EU12 and Eastern Europeans, faced the same legislative framework in all British regions, including London. Rather, London appears to exhibit a cultural milieu of ‘selective cosmopolitanism’. These findings add to the still limited literature on the relationship between public opinion on immigrants (here proxied by the referendum vote) and the levels of ethnic discrimination recorded in field experiments.
World and European Rowing Medallists Pace With Smaller Variation Than Their Competitors
Authors: Mentzoni Fredrik , Losnegard Thomas
Published: December 2021

Purpose: To establish the relation between pacing pattern and performance, within sex, and number of crew members, at the very highest performance level in World class rowing.

Methods: Pacing profiles based on official 500 m split times in 106 A-finals with six contesting boat crews (n = 636 crews), in recent World (2017–2019) and European (2017–2021) championships, were analyzed. The coefficient of variation (CV) and sum of relative differences (SRD) of the split times, and normalized velocities in the four segments of the race, were compared between performance levels, that is, placement (1st–6th), and subgroups based on sex (female or male) and number of crew members (one, two, or four). Statistical tests and resulting p-values and effect sizes (Cohen's d) were used to assess differences between groups.

Results: The pacing profiles of the medallists had smaller variation than those of the non-podium finishers (CV = 1.72% vs. CV = 2.00%; p = 4 × 10−7, d = 0.41). Compared to the non-podium finishers, the medallists had lower normalized velocities in the first and second segments of the race, slightly higher in the third segment and higher in the fourth segment. Female crews paced somewhat more evenly than male crews. No significant differences were found in the evenness of pacing profiles between singles, doubles/pairs and quads/fours. Analyses of SRD were overall consistent with analyses of CV.

Conclusion: Medal winners in major rowing championships use a more even pacing strategy than their final competitors, which could imply that such a strategy is advantageous in rowing.
Issues Relating to the Creation of a Central Database to Support Statistical Property Valuations
Authors: Grosz Gabriella , Herbert Evelyn , Izsák Gábor , Szász Katinka
Published: December 2021

The valuation of real estate collateral is a long-established area of the lending process that is currently undergoing increasingly dynamic development and in which the use of statistical valuation methods is becoming more and more common instead of on-site valuations. The legal conditions for this have been created by amendments to European and national legislation in the past year, but for the method to be truly widely used and operational and to ensure the accuracy of the resulting valuations, access to detailed, accurate, up-to-date and regularly checked data on real estate must be also created. As the databases currently available for Hungarian real estate are very fragmented, in our study, we propose to create a central database that would provide a uniform, up-to-date set of data, by harmonising the existing separate databases. Such a database would help create a level playing field in the market and automate data transfer in a cost-effective, fast and reliable manner. This would greatly facilitate the uptake of statistical valuation methods, supporting the further spread of digitalisation, increasing banking competition, speeding up administration and reducing the cost of lending for all parties.
Intelligent Fault-Tolerant Control for AC/DC Hybrid Power System of More Electric Aircraft
Authors: Xiao Lingfei , Sattarov Robert R. , Liu Peisong , Lin Cong
Published: December 2021

This paper presents a novel intelligent fault-tolerant control method for a kind of more electric aircraft AC/DC hybrid electrical power system, in order to ensure the safe operation of the engine and improve the power supply quality. The more electric aircraft electrical power system was combined with an aircraft engine, two generators, two AC/DC rectifiers, two DC/AC inverters, DC loads, and AC loads. A multi-objective optimization intelligent sliding mode fault-tolerant controller was obtained for aircraft engine with actuator faults. Each of AC/DC rectifiers is 12-pulse autotransformer rectifier unit with active power filter. Active power filter was used to realize the desired performance of DC bus. Intelligent fractional order PI controller is presented for AC/DC rectifier by considering multiple performance indexes. In order to guarantee the AC-side has satisfying voltage, current, and frequency, no matter the sudden change of AC load that happens or DC/AC fault that occurs, the virtual synchronous generator control method was used for DC/AC inverters. Simulation results verify the effective of the proposed more electric aircraft AC/DC hybrid electrical power system.
Production and Profitability of Hybrid Rice Is Influenced by Different Nutrient Management Practices
Authors: Bhatta Ram Datta , Paudel Mahendra , Ghimire Kishor , Dahal Khem Raj , Amgain Lal Prasad , Pokhrel Samiksha , Acharya Samikshya , Kandel Bishnu Prasad , Aryal Krishna , K. C. Bishwas , Pandey Meena , KC Yadav , Paudel Samrat , Subedi Milan , Pant Bhoj Raj , Bajgai Tirtha Raj , Koirala Niranjan , Aboelenin Salama Mostafa , Soliman Mohamed Mohamed , EI-Saber Batiha Gaber , Upadhyaya Jitendra
Published: December 2021

The government of Nepal has recommended blanket fertilizer application for rice cultivation, which results in lower nutrient use efficiency (NUE) particularly under rainfed conditions. With the aim of finding an appropriate nutrient management practices concerning rice production and profitability, a field experiment was conducted during rainy season of 2017 and 2018 at Kavrepalanchowk and Dang district of Nepal. Altogether, five treatments comprising various nutrient management practices viz. Nutrient Expert Model (NE), use of Leaf Color Chart (LCC), Government Recommended Fertilizer Dose (GON), Farm Yard Manure (FYM), and Farmers’ Field Practice (FFP), were laid out in RCBD with four replications in farmers’ fields. The analysis of variance showed significant difference between treatments for test weight and grain yield in Kavrepalanchowk whereas all traits except number of effective tillers were significant in Dang. The significantly higher grain yield and harvest index were obtained in NE, followed by LCC; and the overall straw yield was highest in LCC, followed by NE in both the locations. Also, yield gap analysis suggested the NE had 44.44% and 23.97% increase in yield as compared to FPP in Kavrepalanchowk and Dang, respectively. The combined analysis with Best Linear Unbiased Estimator revealed the interaction of nutrient management and location significantly effects the straw yield and harvest index across both the locations. The estimated mean straw yield and harvest index were 10.93 t/ha and 34.98%, respectively. Both correlation study and biplot of principal component analysis signaled grain yield had positive correlation with all other traits. Furthermore, the net revenue was maximum for NE, followed by LCC in both the locations. The benefit: cost ratio was highest for NE which was 1.55 in Kavrepalanchowk and 2.61 in Dang. On the basis of these findings, NE and LCC can be effectively used as nutrient management practice by the farmers to obtain maximum production and profitability in Rice.
Assessment of Drought Tolerance of Miscanthus Genotypes through Dry-Down Treatment and Fixed-Soil-Moisture-Content Techniques
Authors: Weng Tzu-Ya , Nakashima Taiken , Villanueva-Morales Antonio , Stewart J. Ryan , Sacks Erik J. , Yamada Toshihiko
Published: December 2021

Miscanthus, a high-yielding, warm-season C4 grass, shows promise as a potential bioenergy crop in temperate regions. However, drought may restrain productivity of most genotypes. In this study, total 29 Miscanthus genotypes of East-Asian origin were screened for drought tolerance with two methods, a dry-down treatment in two locations and a system where soil moisture content (SMC) was maintained at fixed levels using an automatic irrigation system in one location. One genotype, Miscanthus sinensis PMS-285, showed relatively high drought-tolerance capacity under moderate drought stress. Miscanthus sinensis PMS-285, aligned with the M. sinensis ‘Yangtze-Qinling’ genetic cluster, had relatively high principal component analysis ranking values in both two locations experiments, Hokkaido University and Brigham Young University. Genotypes derived from the ‘Yangtze-Qinling’ genetic cluster showed relatively greater photosynthetic performance than other genetic clusters, suggesting germplasm from this group could be a potential source of drought-tolerant plant material. Diploid genotypes showed stronger drought tolerance than tetraploid genotypes, suggesting ploidy could be an influential factor for this trait. Of the two methods, the dry-down treatment appears more suitable for selecting drought-tolerant genotypes given that it reflects water-stress conditions in the field. However, the fixed-SMC experiment may be good for understanding the physiological responses of plants to relatively constant water-stress levels.
The Optimal Cultivar × Sowing Date × Plant Density for Grain Yield and Resource Use Efficiency of Summer Maize in the Northern Huang–Huai–Hai Plain of China
Authors: Zhai Lichao , Zhang Lihua , Yao Haipo , Zheng Mengjing , Ming Bo , Xie Ruizhi , Zhang Jingting , Jia Xiuling , Ji Junjie
Published: December 2021

In order to explore the optimal cultivar × sowing date × plant density for summer maize (Zea mays L.) in the Northern Huang–Huai–Hai (HHH) Plain of China, field experiments were conducted over two consecutive years (2018–2019) on a loam soil in the Northern HHH Plain. A split–split plot design was employed in this study, and the main plots included three cultivars (HM1: early-maturing cultivar; ZD958: medium-maturing cultivar; DH605: late-maturing cultivar); subplots consisted of three sowing dates (SD1: June 10; SD2: June 17; SD3: June 24); sub-sub plots include two plant densities (PD1: 6.75 × 104 plants ha−1; PD2: 8.25 × 104 plants ha−1). The results showed that the effects of cultivar and plant density on grain yield of summer maize were not significant, and the sowing date was the major factor affecting the grain yield. Delayed sowing significantly decreased the grain yield of summer maize, this was due mainly to the reduced kernel weight, which is associated with the lower post-anthesis dry matter accumulation. Moreover, radiation use efficiency (RUE), temperature use efficiency (TUE), and water use efficiency (WUE) were significantly affected by cultivar, sowing date, and plant density. Selecting early- and medium-maturing cultivars was beneficial to the improvements in RUE and TUE, and plants grown at earlier sowing with higher plant density increased the RUE and TUE. The interactive analysis of cultivar × sowing date × plant density showed that the optimum grain yields of all tested cultivars were observed at SD1-PD2, and the optimum RUE and TUE for HM1, ZD958, and DH605 were observed at SD1-PD2, SD2-PD2, and SD2-PD2, respectively. The differences in the optimum grain yield, RUE, and TUE among the tested cultivars were not significant. These results suggested that plants grown at earlier sowing with reasonable dense planting had benefits of grain yield and resource use efficiency. In order to adapt to mechanized grain harvesting, early-maturing cultivar with lower grain moisture at harvest would be the better choice. Therefore, adopting early-maturing cultivars grown with earlier sowing with reasonably higher plant density would be the optimal planting pattern for summer maize production in the Northern HHH Plain of China in future.
Effects of Melatonin Treatment on Sweet Cherry Tree Yield and Fruit Quality
Authors: Carrión-Antolí Alberto , Lorente-Mento José Manuel , Valverde Juan Miguel , Castillo Salvador , Valero Daniel , Serrano María
Published: December 2021

The effects of preharvest melatonin treatment, applied as foliar spray at 0.1, 0.3 and 0.5 mM concentration at three key points of fruit development (pit hardening, initial colour changes and 3 days before harvesting), on crop yield and fruit quality properties at harvest was evaluated in three sweet cherry cultivars, ‘Prime Giant’, ‘Lapins’ and ‘Sweet Heart’, and two years, 2019 and 2020. The results showed that melatonin treatment had no effect on crop yield, except for the ‘Lapins’ cultivar, in which increases were found. However, decayed and cracked fruit percentage was decreased in all cultivars in 2020 when adverse weather conditions occurred and commercial crop yield was increased, especially for 0.3 mM dose. Fruit quality traits at harvest, such as fruit weight, colour, firmness, total soluble solids and titratable acidity, were enhanced by melatonin treatments in all sweet cherry cultivars and in both years. Moreover, bioactive compounds, such as total phenolics and total and individual anthocyanins, were also found at higher levels in fruit from melatonin-treated trees with respect to controls. Thus, taking into account all these effects, 0.3 mM melatonin foliar spray, at three key points of fruit developmental stages, could be a useful tool to improve crop yield and quality traits of sweet cherries, especially their content on bioactive compounds with antioxidant properties and health beneficial effects.
The Extended Effect of Top-Dressed Recovered Struvite Fertiliser on Residual Irish Grassland Soil Phosphorus Levels Compared to Commercial Phosphorus Fertiliser
Authors: O’Donnell Ciarán , Barnett Denise , Harrington Joe , Power Niamh
Published: December 2021

Phosphorus (P) is an essential growth-limiting nutrient that cannot be synthetically produced. Mineral P fertilisers are typically applied to crops to sustain modern farming practice and food production. These fertilisers are generally derived from finite phosphate ore, for which there is much concern over long-term sustainability. To address these concerns, various technologies have been developed to recover P from municipal wastewater treatment plants. One product recovered from these processes is struvite, which is a precipitate formed of magnesium, ammonium, and phosphate. To analyse the fertilisation value of the recovered struvite, field trials were conducted on perennial ryegrass (Lolium perenne) over three growing seasons, analysing the dry matter yield of recovered struvite fertiliser. The trial was based on a three-crop silage system designed to mimic typical Irish agricultural practice. This research highlights that recovered struvite as a fertiliser provides additional benefits including increasing the soil P levels. The struvite test case produced a statistically significantly increased soil P level from the baseline of Morgan’s extractable P content of 6.4 mg/L to the optimum Morgan’s soil P level of 11.13 mg/L. The findings of this research provide insight into the added benefits of recovered struvite fertiliser as a sustainable renewable P fertiliser.
The Effect of Rearing Conditions on Carcass Traits, Meat Quality and the Compositions of Fatty Acid and Amino Acid of LTL in Heigai Pigs
Authors: Li Jie , Liu Jiaqi , Zhang Shu , Xie Jintang , Shan Tizhong
Published: December 2021

The present study evaluates the influence of captivity and grazing rearing conditions on the carcass traits, meat quality and fatty acid profiles of Heigai pigs. Ten Heigai pigs with market weight were randomly selected from both the indoor feeding farm and outdoor grazing farm groups (FF and GF; five pigs per group) for measuring production performance. The results showed that the shear force of longissimus thoracis et lumborum (LTL) in the GF group tended to increase (p = 0.06), and triglyceride and cholesterol contents in LTL and psoas major muscle (PMM) of the GF group significantly increased and decreased, respectively (p < 0.05). The proportion of saturated fatty acids (SFA) was significantly increased (p < 0.05) in the GF group. Meanwhile, the ratios of unsaturated fatty acid (UFA), polyunsaturated fatty acid (PUFA), monounsaturated fatty acid (MUFA) and the content of flavor amino acid of the LTL in the GF group were significantly decreased (p < 0.05). The GF upregulated the expression of MyHC-IIb and lipogenic genes, such as GLUT4 and LPL (p < 0.05), in LTL and PMM, but downregulated the expression of MyHC-I, MyHC-IIa, PPARγ and leptin (p < 0.05). In conclusion, these results suggested that the different rearing conditions can alter the meat qualities by mediating the muscle fiber type and lipid metabolism of Heigai pigs.
Effect of Artificial Regime Shifts and Biotic Factors on the Intensity of Foraging of Planktivorous Fish
Authors: Ciszewski Krzysztof , Wawrzyniak Wawrzyniec , Czerniejewski Przemysław
Published: December 2021

It is still to be confirmed whether global warming with its predicted elevated water temperature will cause an increase in predation and alter phenological and physiological processes leading to changes in the size of aquatic organisms. In an experimental system of water column stratification simulating a natural combination of field conditions, we created artificial abiotic factors that mimicked the natural environment, i.e., light intensity, oxygen conditions, and thermal stratification. Subsequently, we added biotic factors such as algae, Daphnia, and planktivorous fish. We studied the intensity of foraging of planktivorous fish on individuals of Daphnia per min in different conditions of biotic and abiotic gradients. We demonstrated a possible scenario involving the risk of elimination of large prey within macrocladocera communities by predatory pressure as a result of climate change. A higher intensity of foraging of planktivorous fish caused or increased the occurrence of larger groups of planktonic animals with a smaller body size. The mechanisms of a future scenario were discovered at a higher trophic level in the aquatic environment.
Evaluation of Antibiotic Resistance of Salmonella Serotypes and Whole-Genome Sequencing of Multiresistant Strains Isolated from Food Products in Russia
Authors: Rakitin Andrey L. , Yushina Yulia K. , Zaiko Elena V. , Bataeva Dagmara S. , Kuznetsova Oksana A. , Semenova Anastasia A. , Ermolaeva Svetlana A. , Beletskiy Aleksey V. , Kolganova Tat’yana V. , Mardanov Andrey V. , Shapovalov Sergei O. , Tkachik Timofey E.
Published: December 2021

Food products may be a source of Salmonella, one of the main causal agents of food poisoning, especially after the emergence of strains resistant to antimicrobial preparations. The present work dealt with investigation of the occurrence of resistance to antimicrobial preparations among S. enterica strains isolated from food. The isolates belonged to 11 serovars, among which Infantis (28%), Enteritidis (19%), and Typhimurium (13.4%) predominated. The isolates were most commonly resistant to trimethoprim/sulfamethoxazole (n = 19, 59.38%), cefazolin (n = 15, 46.86%), tetracycline (n = 13, 40.63%), and amikacin (n = 9, 28.13%). Most of the strains (68.75%) exhibited multiple resistance to commonly used antibiotics. High-throughput sequencing was used to analyse three multidrug-resistant strains (resistant to six or more antibiotics). Two of them (SZL 30 and SZL 31) belonged to S. Infantis, while one strain belonged to S. Typhimurium (SZL 38). Analysis of the genomes of the sequenced strains revealed the genes responsible for antibiotic resistance. In the genomes of strains SZL 30 and SZL 31 the genes of antibiotic resistance were shown to be localized mostly in integrons within plasmids, while most of the antibiotic resistance genes of strain SZL 38 were localized in a chromosomal island (17,949 nt). Genomes of the Salmonella strains SZL 30, SZL 31, and SZL 38 were shown to contain full-size pathogenicity islands: SPI-1, SPI-2, SPI-4, SPI-5, SPI-9, SPI-11, SPI-13, SPI-14, and CS54. Moreover, the genome of strain SZL 38 was also found to contain the full-size pathogenicity islands SPI-3, SPI-6, SPI-12, and SPI-16. The emergence of multidrug-resistant strains of various Salmonella serovars indicates that further research on the transmission pathways for these genetic determinants and monitoring of the distribution of these microorganisms are necessary.
Cefiderocol for Severe Carbapenem-Resistant A. baumannii Pneumonia: Towards the Comprehension of Its Place in Therapy
Authors: Rando Emanuele , Segala Francesco Vladimiro , Vargas Joel , Seguiti Cristina , De Pascale Gennaro , Murri Rita , Fantoni Massimo
Published: December 2021

Cefiderocol use in A. baumannii pneumonia still represents an important matter of debate. The aim of this study is to describe 13 cases of carbapenem-resistant A. baumannii (CRAB) pneumonia treated with cefiderocol in real-life practice. We retrospectively included patients with CRAB pneumonia hospitalized at Fondazione Policlinico Universitario Agostino Gemelli Hospital treated with cefiderocol either in the general ward or the intensive care unit. A total of 11 patients out of 13 had ventilator-associated pneumonia caused by CRAB, and 12/13 patients had polymicrobial infection. We found a 30-day success rate of 54%. Cefiderocol may have a role when facing severe XDR A. baumannii pneumonia. Future studies are warranted to better define its place in therapy in CRAB infections.
Anti-Staphylococcal Activity of Cinnamomum zeylanicum Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections
Authors: Albuquerque Vinicius de Queiroz , Soares Maria Janeila Carvalho , Matos Maria Nágila Carneiro , Cavalcante Rafaela Mesquita Bastos , Guerrero Jesús Alberto Pérez , Soares Rodrigues Tigressa Helena , Gomes Geovany Amorim , de Medeiros Guedes Rodrigo Fonseca , Castelo-Branco Débora de Souza Collares Maia , Goes da Silva Isaac Neto , Carneiro Victor Alves
Published: December 2021

The aim of this study was to evaluate the phytochemical profile of Cinnamomum zeylanicum essential oil (CZEO) and their antimicrobial and antibiofilm activity against Staphylococcus strains isolated from canine otitis. First, the CZEO chemical composition was determined by gas chromatography-mass spectrometry (CG-MS). External otitis samples collected from dogs were submitted to staphylococcal isolation, followed by MALDI-TOF mass spectrometry identification. The antimicrobial action was tested against the isolates using the disk-diffusion and microdilution methods. The antibiofilm activity was evaluated by CZEO-based concentrations, subMIC for biofilm formation and supraMIC against preformed biofilm, quantified by crystal violet (CV) staining and CFU counting. The chemical analysis revealed that (E)-cinnamaldehyde, eugenol and (E)-cinnamyl acetate were the main compounds in the CZEO, representing 77.42, 8.17 and 4.50%, respectively. Two strains of three different species, S. saprophyticus, S. schleiferi and S. pseudintermedius, were identified. The disk-diffusion test showed an inhibitory zone diameter, ranging from 34.0 to 49.5 mm, while the MIC and MBC values were around 500 and 1000 µg/mL. SubMIC demonstrated an inhibition on biofilm formation against 4 out the 6 strains tested. On mature biofilm, the CZEO-based supraMIC groups had slightly change on biomass, however, the biofilm cell viability decreased the CFU in 3 magnitude orders.
Knowledge, Awareness and Practice with Antimicrobial Stewardship Programmes among Healthcare Providers in a Ghanaian Tertiary Hospital
Authors: Kpokiri Eneyi E. , Ladva Misha , Dodoo Cornelius C. , Orman Emmanuel , Aku Thelma Alalbila , Mensah Adelaide , Jato Jonathan , Mfoafo Kwadwo A. , Folitse Isaac , Hutton-Nyameaye Araba , Okon-Ben Inemesit , Mensah-Kane Paapa , Sarkodie Emmanuel , Awadzi Benedict , Jani Yogini H.
Published: December 2021

Antimicrobial resistance (AMR) is a significant problem in global health today, particularly in low- and middle-income countries (LMICs) where antimicrobial stewardship programmes are yet to be successfully implemented. We established a partnership between AMR pharmacists from a UK NHS hospital and in Ho Teaching Hospital with the aim of enhancing antimicrobial stewardship knowledge and practice among healthcare providers through an educational intervention. We employed a mixed-method approach that included an initial survey on knowledge and awareness before and after training, followed by qualitative interviews with healthcare providers conducted six months after delivery of training. This study was carried out in two phases in Ho Teaching Hospital with healthcare professionals, including pharmacists, medical doctors, nurses and medical laboratory scientists. Ethical approval was obtained prior to data collection. In the first phase, we surveyed 50 healthcare providers, including nurses (33%), pharmacists (29%) and biomedical scientists (23%). Of these, 58% of participants had engaged in continuous professional development on AMR/AMS, and above 95% demonstrated good knowledge on the general use of antibiotics. A total of 18 participants, which included four medical doctors, five pharmacists, four nurses, two midwives and three biomedical scientists, were interviewed in the second phase and demonstrated greater awareness of AMS practices, particularly the role of education for patients, as well as healthcare professionals. We found that knowledge and practice with AMS was markedly improved six months after the training session. There is limited practice of AMS in LMICs; however, through AMR-focused training, we demonstrated improved AMS skills and practice among healthcare providers in Ho Teaching Hospital. There is a need for continuous AMR training sessions for healthcare professionals in resource-limited settings.
Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR Klebsiella pneumoniae on Antimicrobial Stress
Authors: Fursova Anastasiia D. , Fursov Mikhail V. , Astashkin Evgenii I. , Novikova Tatiana S. , Fedyukina Galina N. , Kislichkina Angelina A. , Alexandrova Irina A. , Ershova Olga N. , Dyatlov Ivan A. , Fursova Nadezhda K.
Published: December 2021

Klebsiella pneumoniae is an increasingly important hospital pathogen. Classical K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of K. pneumoniae resulted in the generation of hybrid hvKP-MDR strains. K. pneumoniae distinct isolates (n = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014–2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395K47 and ST147K64), hvKp (ST86K2), and hvKp-MDR (ST23K1 and ST23K57) revealed blaSHV, blaTEM, blaCTX, blaOXA-48, and blaNDM beta-lactamase genes; acr, oqx, kpn, kde, and kex efflux genes; and K. pneumoniae virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens.
Adjunctive Effect of Systemic Antibiotics in Regenerative/Reconstructive Periodontal Surgery—A Systematic Review with Meta-Analysis
Authors: Nibali Luigi , Buti Jacopo , Barbato Luigi , Cairo Francesco , Graziani Filippo , Jepsen Søren
Published: December 2021

Background and Objective: Systemic antibiotics (AB) are often used in conjunction with regenerative/reconstructive periodontal surgery of intrabony defects and furcations; however, their potential benefits have not been systematically assessed. Materials and Methods: Data were retrieved from two recent systematic reviews (a total of 105 randomized clinical trials (RCTs) on clinical and radiographic outcomes in intrabony defects (ID) and molars with furcation involvement (FI) treated by surgical access with regenerative techniques. Pair-wise meta-analysis of RCTs with and without AB was performed. Meta-regressions from single-arm (subgroup) RCTs including study arms with or without adjunctive AB were also conducted. Results: No statistically significant benefits of systemic AB with regard to PPD, CAL and bone gain were detected in ID by pair-wise meta-analysis. Meta-regression revealed increased PPD reduction (−0.91 mm, 95% CI = −1.30; −0.51, p < 0.001), CAL gain (−0.92 mm, 95% CI = −1.32; −0.52, p < 0.001) and bone gain (−1.08 mm, 95% CI = −1.63; −0.53, p < 0.001) in ID but not in any of the outcomes in FI for arms treated with AB vs. study arms treated with no AB. No clear differences in adverse events were detected between AB and non-AB groups. Conclusion: There is only weak indirect evidence that AB may provide additional benefits in terms of clinical improvements in the regenerative/reconstructive periodontal surgery of intrabony defects and no evidence for a benefit in furcations. Until new data are gained and in the context of antibiotic stewardship, it may be questionable to justify the adjunctive use of systemic antibiotics.