bluuebunny/crossref_metadata_2025 huggingface dataset:
Old and New Players of Inflammation and Their Relationship With Cancer Development
Authors: Chavez-Dominguez Rodolfo , Perez-Medina Mario , Aguilar-Cazares Dolores , Galicia-Velasco Miriam , Meneses-Flores Manuel , Islas-Vazquez Lorenzo , Camarena Angel , Lopez-Gonzalez Jose S.
Published: November 2021

Pathogens or genotoxic agents continuously affect the human body. Acute inflammatory reaction induced by a non-sterile or sterile environment is triggered for the efficient elimination of insults that caused the damage. According to the insult, pathogen-associated molecular patterns, damage-associated molecular patterns, and homeostasis-altering molecular processes are released to facilitate the arrival of tissue resident and circulating cells to the injured zone to promote harmful agent elimination and tissue regeneration. However, when inflammation is maintained, a chronic phenomenon is induced, in which phagocytic cells release toxic molecules damaging the harmful agent and the surrounding healthy tissues, thereby inducing DNA lesions. In this regard, chronic inflammation has been recognized as a risk factor of cancer development by increasing the genomic instability of transformed cells and by creating an environment containing proliferation signals. Based on the cancer immunoediting concept, a rigorous and regulated inflammation process triggers participation of innate and adaptive immune responses for efficient elimination of transformed cells. When immune response does not eliminate all transformed cells, an equilibrium phase is induced. Therefore, excessive inflammation amplifies local damage caused by the continuous arrival of inflammatory/immune cells. To regulate the overstimulation of inflammatory/immune cells, a network of mechanisms that inhibit or block the cell overactivity must be activated. Transformed cells may take advantage of this process to proliferate and gradually grow until they become preponderant over the immune cells, preserving, increasing, or creating a microenvironment to evade the host immune response. In this microenvironment, tumor cells resist the attack of the effector immune cells or instruct them to sustain tumor growth and development until its clinical consequences. With tumor development, evolving, complex, and overlapping microenvironments are arising. Therefore, a deeper knowledge of cytokine, immune, and tumor cell interactions and their role in the intricated process will impact the combination of current or forthcoming therapies.
Alpha-L-Fucosidase Has Diagnostic Value in Prostate Cancer With “Gray-Zone PSA” and Inhibits Cancer Progression via Regulating Glycosylation
Authors: Zhang Cong , Liu Jikai , Chao Fan , Wang Shiyu , Li Dawei , Han Dunsheng , Xu Zhonghua , Xu Guoxiong , Chen Gang
Published: November 2021

Background

This study aimed to explore the diagnostic value of alpha-l-fucosidase (AFU) in prostate cancer (PCa) patients with “gray-zone PSA” and to investigate the correlation between AFU expression and clinicopathological characteristics of PCa patients.

Methods

The level of AFU and other necessary clinicopathological variables of patients were retrieved from electronic medical records. The transcriptome profiling and clinical information of PCa patients were obtained from The Cancer Genome Atlas (TCGA) database. The protein level of AFU in tissue was assessed by immunohistochemistry (IHC). All the data were processed by appropriate analysis methods. The p-value of <0.05 was considered statistically significant.

Results

AFU showed ideal diagnostic value for PCa with prostate-specific antigen (PSA) levels ranging from 4 to 10 ng/ml, and its optimal cutoffs were 19.5 U/L. Beyond this, low AFU expression was associated with high pathological grade, T stage and N stage, more postoperative residual tumors, and poor primary therapy outcome, as well as shorter progression-free interval. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis illustrated that FUCA1/FUCA2 exerted tumor-suppressive function by regulating the glycosylation.

Conclusions

AFU (<19.5 U/L) could effectively distinguish the PCa from the patients with “gray-zone PSA”, and low expression of AFU was an independent unfavorable predictor for the clinicopathological characteristics of PCa patients.
Doxorubicin/Adriamycin Monotherapy or Plus Ifosfamide in First-Line Treatment for Advanced Soft Tissue Sarcoma: A Pooled Analysis of Randomized Trials
Authors: Wang Bi-Cheng , Kuang Bo-Hua , Xiao Bo-Ya , Lin Guo-He
Published: November 2021

Background

Doxorubicin/Adriamycin (ADM) alone or combined with ifosfamide (IFO) (AI) is available for previously untreated advanced soft tissue sarcoma (ASTS). However, the clinical choice between them remains controversial. In this pooled analysis, we comprehensively compared the efficacy and tolerability of AI versus ADM in patients with ASTS.

Methods

PubMed, Web of Science, EMBASE, and Cochrane Library were systematically searched from inception to April 14, 2021. Eligible studies were randomized clinical trials comparing AI to ADM. The primary outcomes were overall survival (OS), progression-free survival (PFS), and objective response rate (ORR). Discontinuation rate (DR) and toxic death (TD) were explored as secondary outcomes.

Results

Overall, three open-label randomized phase 2/3 clinical trials with a total of 1108 newly diagnosed ASTS patients were enrolled. Between AI and ADM, pooled hazard ratios were 0.93 (95% confidence interval 0.58-1.50, p = 0.78) for OS and 0.85 (0.57-1.25, p = 0.41) for PFS. While pooled risk ratios for ORR, DR, and TD were 1.37 (0.94-1.99, p = 0.10), 1.04 (0.74-1.46, p = 0.82), and 0.68 (0.19-2.36, p = 0.54) respectively. No publication bias was observed across the studies.

Conclusion

In the first-line setting, adding IFO to ADM failed to benefit ASTS patients against ADM alone, even with comparable tolerability.
Estrogen Receptor 1 Inhibition of Wnt/β-Catenin Signaling Contributes to Sex Differences in Hepatocarcinogenesis
Authors: Bhat Mamatha , Pasini Elisa , Pastrello Chiara , Angeli Marc , Baciu Cristina , Abovsky Mark , Coffee Angella , Adeyi Oyedele , Kotlyar Max , Jurisica Igor
Published: November 2021

Background

Hepatocellular Carcinoma (HCC) is a sexually dimorphic cancer, with female sex being independently protective against HCC incidence and progression. The aim of our study was to understand the mechanism of estrogen receptor signaling in driving sex differences in hepatocarcinogenesis.

Methods

We integrated 1,268 HCC patient sample profiles from publicly available gene expression data to identify the most differentially expressed genes (DEGs). We mapped DEGs into a physical protein interaction network and performed network topology analysis to identify the most important proteins. Experimental validation was performed in vitro on HCC cell lines, in and in vivo, using HCC mouse model.

Results

We showed that the most central protein, ESR1, is HCC prognostic, as increased ESR1 expression was protective for overall survival, with HR=0.45 (95%CI 0.32-0.64, p=4.4E-06), and was more pronounced in women. Transfection of HCC cell lines with ESR1 and exposure to estradiol affected expression of genes involved in the Wnt/β-catenin signaling pathway. ER-α (protein product of ESR1) agonist treatment in a mouse model of HCC resulted in significantly longer survival and decreased tumor burden (p<0.0001), with inhibition of Wnt/β-Catenin signaling. In vitro experiments confirmed colocalization of β-catenin with ER-α, leading to inhibition of β-catenin-mediated transcription of target genes c-Myc and Cyclin D1.

Conclusion

Combined, the centrality of ESR1 and its inhibition of the Wnt/β-catenin signaling axis provide a biological rationale for protection against HCC incidence and progression in women.
Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson’s Disease Rats
Authors: Zhou Li , Zheng Li-Fei , Zhang Xiao-Li , Wang Zhi-Yong , Yao Yuan-Sheng , Xiu Xiao-Lin , Liu Chen-Zhe , Zhang Yue , Feng Xiao-Yan , Zhu Jin-Xia
Published: November 2021

The cholinergic anti-inflammatory pathway (CAIP) has been proposed to regulate gastrointestinal inflammation via acetylcholine released from the vagus nerve activating α7 nicotinic receptor (α7nAChR) on macrophages. Parkinson’s disease (PD) patients and PD rats with substantia nigra (SN) lesions exhibit gastroparesis and a decayed vagal pathway. To investigate whether activating α7nAChR could ameliorate inflammation and gastric dysmotility in PD rats, ELISA, western blot analysis, and real-time PCR were used to detect gastric inflammation. In vitro and in vivo gastric motility was investigated. Proinflammatory mediator levels and macrophage numbers were increased in the gastric muscularis of PD rats. α7nAChR was located on the gastric muscular macrophages of PD rats. The α7nAChR agonists PNU-282987 and GTS-21 decreased nuclear factor κB (NF-κB) activation and monocyte chemotactic protein-1 mRNA expression in the ex vivo gastric muscularis of PD rats, and these effects were abolished by an α7nAChR antagonist. After treatment with PNU-282987 in vivo, the PD rats showed decreased NF-κB activation, inflammatory mediator production, and contractile protein expression and improved gastric motility. The present study reveals that α7nAChR is involved in the development of gastroparesis in PD rats and provides novel insight for the treatment of gastric dysmotility in PD patients.
Competition Between Red Blood Cell Aggregation and Breakup: Depletion Force due to Filamentous Viruses vs. Shear Flow
Authors: Korculanin O. , Kochetkova T. , Lettinga M. P.
Published: November 2021

Human blood is a shear-thinning fluid with a complex response that strongly depends on the red blood cell’s (RBC’s) ability to form aggregates, called rouleaux. Despite numerous investigations, microscopic understanding of the break up of RBC aggregates has not been fully elucidated. Here, we present a study of breaking up aggregates consisting of two RBCs (a doublet) during shear flow. We introduce the filamentous fd bacteriophage as a rod-like depletant agent with a very long-range interaction force, which can be tuned by the rod’s concentration. We visualize the structures while shearing by combining a home-build counter-rotating cone-plate shear cell with microscopy imaging. A diagram of dynamic states for shear rates versus depletant concentration shows regions of different flow responses and separation stages for the RBCs doublets. With increasing interaction forces, the full-contact flow states dominate, such as rolling and tumbling. We argue that the RBC doublets can only undergo separation during tumbling motion when the angle between the normal of the doublets with the flow direction is within a critical range. However, at sufficiently high shear rates, the time spent in the critical range becomes too short, such that the cells continue to tumble without separating.
Almost Periodic Solutions to Impulsive Stochastic Delay Differential Equations Driven by Fractional Brownian Motion With 12 < H < 1
Authors: Gao Lili , Sun Xichao
Published: November 2021

In this article, we study the existence and uniqueness of square-mean piecewise almost periodic solutions to a class of impulsive stochastic functional differential equations driven by fractional Brownian motion. Moreover, the stability of the mild solution is obtained. To illustrate the results obtained in the paper, an impulsive stochastic functional differential equation driven by fractional Brownian motion is considered.
Prediction of Survival After Partial Hepatectomy Using a Physiologically Based Pharmacokinetic Model of Indocyanine Green Liver Function Tests
Authors: Köller Adrian , Grzegorzewski Jan , Tautenhahn Hans-Michael , König Matthias
Published: November 2021

The evaluation of hepatic function and functional capacity of the liver are essential tasks in hepatology as well as in hepatobiliary surgery. Indocyanine green (ICG) is a widely applied test compound that is used in clinical routine to evaluate hepatic function. Important questions for the functional evaluation with ICG in the context of hepatectomy are how liver disease such as cirrhosis alters ICG elimination, and if postoperative survival can be predicted from preoperative ICG measurements. Within this work a physiologically based pharmacokinetic (PBPK) model of ICG was developed and applied to the prediction of the effects of a liver resection under various degrees of cirrhosis. For the parametrization of the computational model and validation of model predictions a database of ICG pharmacokinetic data was established. The model was applied (i) to study the effect of liver cirrhosis and liver resection on ICG pharmacokinetics; and (ii) to evaluate the model-based prediction of postoperative ICG-R15 (retention ratio 15 min after administration) as a measure for postoperative outcome. Key results are the accurate prediction of changes in ICG pharmacokinetics caused by liver cirrhosis and postoperative changes of ICG-elimination after liver resection, as validated with a wide range of data sets. Based on the PBPK model, individual survival after liver resection could be classified, demonstrating its potential value as a clinical tool.
Effect of Long-Term Exercise on Liver Lipid Metabolism in Chinese Patients With NAFLD: A Systematic Review and Meta-Analysis
Authors: Gao Ye , Lu Jiandong , Liu Xinhong , Liu Jingqi , Ma Qirui , Shi Yajun , Su Hao
Published: November 2021

Purpose: Using meta-analysis to evaluate the effect of various long-term exercises (more than 4 weeks) on liver lipid metabolism of Chinese patients with non-alcoholic fatty liver disease (NAFLD) and provides more targeted exercise recommendations.

Methods: Four databases consisting of PubMed, Web of Science, China Science and Technology Journal Database (VIP), China Knowledge Resource Integrated Database (CNKI) were searched up to May 2021. Randomized controlled trials (RCTs) were eligible, and the outcomes of body composition, lipid metabolism [including triglyceride (TG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), and high-density lipoprotein-cholesterol (HDL-C)], and liver function [including alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] were used to assess the effectiveness of long-term exercise on Chinese patients with NAFLD.

Results: Eleven articles with a total of 13 data points (involving 1,006 participants) satisfied the inclusion criteria and were pooled in the meta-analysis. The findings demonstrated that long-term exercise decreased the level of TG [−0.50, 95%CI (−0.64, −0.36)], TC [−0.55, 95%CI (−0.92, −0.18)], LDL-C [−0.29, 95%CI (−0.43, −0.15)], ALT [−3.45, 95%CI (−6.78, −0.12)], AST [−6.91, 95%CI (−10.00, −3.81)], and body mass index (BMI) of patients who did exercise last more than 6 months [−1.55, 95%CI (−2.32, −0.79)] significantly. The effect on HDL-C was not obvious.

Conclusion: Long-term exercise can improve the levels of TG, TC, LDL-C, ALT, and AST in Chinese patients with NAFLD significantly, and exercise last more than 6 months can decrease the BMI of Chinese patients with NAFLD.
Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
Authors: Sun Xuezhao , Andrew Ian G. , Harris Philip J. , Hoskin Simone O. , Joblin Keith N. , He Yuhua
Published: November 2021

The cell walls of forage chicory (Cichorium intybus) leaves are known to contain high proportions of pectic polysaccharides. However, little is known about the distribution of pectic polysaacharides among walls of different cell types/tissues and within walls. In this study, immunolabelling with four monoclonal antibodies was used to map the distribution of pectic polysaccharides in the cell walls of the laminae and midribs of these leaves. The antibodies JIM5 and JIM7 are specific for partially methyl-esterified homogalacturonans; LM5 and LM6 are specific for (1→4)-β-galactan and (1→5)-α-arabinan side chains, respectively, of rhamnogalacturonan I. All four antibodies labelled the walls of the epidermal cells with different intensities. JIM5 and JIM7, but not LM5 or LM6, labelled the middle lamella, tricellular junctions, and the corners of intercellular spaces of ground, xylem and phloem parenchyma. LM5, but not LM6, strongly labelled the walls of the few sclerenchyma fibres in the phloem of the midrib and lamina vascular bundles. The LM5 epitope was absent from some phloem parenchyma cells. LM6, but not LM5, strongly labelled the walls of the stomatal guard cells. The differential distribution of pectic epitopes among walls of different cell types and within walls may reflect the deposition and modification of these polysaccharides which are involved in cell wall properties and cell development.
GrTCP11, a Cotton TCP Transcription Factor, Inhibits Root Hair Elongation by Down-Regulating Jasmonic Acid Pathway in Arabidopsis thaliana
Authors: Hao Juan , Lou Panpan , Han Yidie , Chen Zhehao , Chen Jianmei , Ni Jun , Yang Yanjun , Jiang Zhifang , Xu Maojun
Published: November 2021

TCP transcription factors play important roles in diverse aspects of plant development as transcriptional activators or repressors. However, the functional mechanisms of TCPs are not well understood, especially in cotton fibers. Here, we identified a total of 37 non-redundant TCP proteins from the diploid cotton (Gossypium raimondii), which showed great diversity in the expression profile. GrTCP11, an ortholog of AtTCP11, was preferentially expressed in cotton anthers and during fiber initiation and secondary cell wall synthesis stages. Overexpression of GrTCP11 in Arabidopsis thaliana reduced root hair length and delayed flowering. It was found that GrTCP11 negatively regulated genes involved in jasmonic acid (JA) biosynthesis and response, such as AtLOX4, AtAOS, AtAOC1, AtAOC3, AtJAZ1, AtJAZ2, AtMYC2, and AtERF1, which resulted in a decrease in JA concentration in the overexpressed transgenic lines. As with the JA-deficient mutant dde2-2, the transgenic line 4-1 was insensitive to 50 μM methyl jasmonate, compared with the wild-type plants. The results suggest that GrTCP11 may be an important transcription factor for cotton fiber development, by negatively regulating JA biosynthesis and response.
Origin of Intra-annual Density Fluctuations in a Semi-arid Area of Northwestern China
Authors: Gao Jiani , Rossi Sergio , Yang Bao
Published: November 2021

Intra-annual density fluctuation (IADF) is a structural modification of the tree ring in response to fluctuations in the weather. The expected changes in monsoon flow would lead to heterogeneous moisture conditions during the growing season and increase the occurrence of IADF in trees of the arid ecosystems of continental Asia. To reveal the timings and physiological mechanisms behind IADF formation, we monitored cambial activity and wood formation in Chinese pine (Pinus tabuliformis) during 2017–2019 at three sites in semi-arid China. We compared the dynamics of xylem formation under a drought event, testing the hypothesis that drought affects the process of cell enlargement and thus induces the production of IADF. Wood microcores collected weekly from April to October were used for anatomical analyses to estimate the timings of cambial activity, and the phases of enlargement, wall thickening, and lignification of the xylem. The first cells started enlargement from late April to early May. The last latewood cells completed differentiation in mid-September. Trees produced IADF in 2018. During that year, a drought in June limited cell production in the cambium, only 36% of the xylem cells being formed in IADF trees, compared to 68% in normal tree rings. IADF cells enlarged under drought in early July and started wall thickening during the rainfall events of late July. The drought restricted cell enlargement and affected wall thickening, resulting in narrow cells with wide walls. Cambium and cell enlargement recovered from the abundant rainfall, producing a new layer with large earlywood tracheids. IADF is a specific adaptation of trees to cope with water deficit events occurring during xylem formation. Our findings confirmed the hypothesis that the June-July drought induces latewood-like IADFs by limiting the process of cell enlargement in the xylem. Our finding suggests a higher occurrence of IADF in trees of arid and semi-arid climates of continental Asia if the changes to monsoon flows result in more frequent drought events during the earlywood formation in June.
The Emergence of Mathematical Understanding: Connecting to the Closest Superordinate and Convertible Concepts
Authors: Yang Zezhong , Yang Xintong , Wang Kai , Zhang Yanqing , Pei Guanggang , Xu Bin
Published: November 2021

This study aimed to examine the specific means and internal processes through which mathematical understanding is achieved by focusing on the process of understanding three new mathematical concepts. For this purpose interviews were conducted with 54 junior high school students. The results revealed that mathematical understanding can be achieved when new concepts are connected to at least two existing concepts within a student’s cognitive structure of. One of these two concepts should be the superordinate concept of the new concept or, more accurately, the superordinate concept that is closest to the new concept. The other concept should be convertible, so that a specific example can be derived by changing or transforming its examples. Moreover, the process of understanding a new concept was found to involve two processes, namely, “going” and “coming.” “Going” refers to the process by which a connection is established between a new concept and its closest superordinate concept. In contrast, “coming” is a process by which a connection is established between an existing convertible concept and a new concept. Therefore the connection leading to understanding should include two types of connections: belonging and transforming. These new findings enrich the literature on mathematical understanding and encourage further exploration. The findings suggest that, in order to help students fully understand new mathematical concepts, teachers should first explain the definition of a given concept to students and subsequently teach them how to create a specific example based on examples of an existing concept.
Co-created Future Scenarios as a Tool to Communicate Sustainable Development in Coastal Communities in Palawan, Philippines
Authors: Richter Isabell , Sumeldan Joel , Avillanosa Arlene , Gabe-Thomas Elizabeth , Creencia Lota , Pahl Sabine
Published: November 2021

Scenarios can be used to communicate potential future changes and engage and connect different audiences in exploring sustainable solutions. Communicating scenarios using creative visualisation, co-creation and a focus on local contexts are especially promising. This research is conducted on the island of Palawan in the Philippines as part of the GCRF Blue Communities project. With a quasi-experimental design, we investigate the psychological and emotional effects of the engagement with future scenarios as a tool for communicating sustainability. Together with local stakeholders and community members, three distinct, locally relevant scenario narratives (Business as Usual, Best Case, and Worst Case) have been co-created. Subsequently, a sample of N = 109 local high school students was asked to creatively engage with these scenario narratives. Intentions to engage in sustainable behaviour, perceived behavioural control, ascription of responsibility, consideration of future consequences, six basic emotions and connectedness to place were assessed before and after the activity via paper-pencil administrated questionnaires. A mixed-model analysis showed significant increases in intentions to engage in sustainable behaviour, however, this increase disappeared when consideration of future consequences was added as a covariate, suggesting a mediating effect. The level of consideration of future consequences also increased significantly after engaging with any of the future scenarios, which questions the common interpretation of consideration of future consequences as a trait variable. Perceived behavioural control significantly increased following the engagement with each of the scenarios whereas ascription of responsibility and connectedness to place did not show any changes. Overall, the two most emotion-evoking scenarios, Best Case Scenario and Worst Case Scenario, turn out as superior over the Business as Usual Scenario, which points to the relevance of emotional framing for effective messaging in our sample. This is the first systematic, quantitative assessment of the effects of future scenarios as a communication tool.
The Creative Neurons
Authors: Flinn Mark V.
Published: November 2021

Creativity generates novel solutions to tasks by processing information. Imagination and mental representations are part of the creative process; we can mull over ideas of our own making, and construct algorithms or scenarios from them. Social scenario-building can be viewed as a human cognitive “super-power” that involves abstraction, meta-representation, time-travel, and directed imaginative thought. We humans have a “theater in our minds” to play out a near-infinite array of social strategies and contingencies. Here we propose an integrative model for why and how humans evolved extraordinary creative abilities. We posit that a key aspect of hominin evolution involved relatively open and fluid social relationships among communities, enabled by a unique extended family structure similar to that of contemporary hunter-gatherer band societies. Intercommunity relationships facilitated the rapid flow of information—“Culture”—that underpinned arms-races in information processing, language, imagination, and creativity that distinguishes humans from other species.
Assessing Deception in Questionnaire Surveys With Eye-Tracking
Authors: Fang Xinyue , Sun Yiteng , Zheng Xinyi , Wang Xinrong , Deng Xuemei , Wang Mei
Published: November 2021

Deceit often occurs in questionnaire surveys, which leads to the misreporting of data and poor reliability. The purpose of this study is to explore whether eye-tracking could contribute to the detection of deception in questionnaire surveys, and whether the eye behaviors that appeared in instructed lying still exist in spontaneous lying. Two studies were conducted to explore eye movement behaviors in instructed and spontaneous lying conditions. The results showed that pupil size and fixation behaviors are both reliable indicators to detect lies in questionnaire surveys. Blink and saccade behaviors do not seem to predict deception. Deception resulted in increased pupil size, fixation count and duration. Meanwhile, respondents focused on different areas of the questionnaire when lying versus telling the truth. Furthermore, in the actual deception situation, the linear support vector machine (SVM) deception classifier achieved an accuracy of 74.09%. In sum, this study indicates the eye-tracking signatures of lying are not restricted to instructed deception, demonstrates the potential of using eye-tracking to detect deception in questionnaire surveys, and contributes to the questionnaire surveys of sensitive issues.
Physiotherapy and Physical Activity as Factors Improving the Psychological State of Patients With Cancer
Authors: Zyzniewska-Banaszak Ewelina , Kucharska-Mazur Jolanta , Mazur Aleksandra
Published: November 2021

Physiotherapy in oncology is a process closely related to cancer treatment methods. Rehabilitation is based on physical activity in various forms involving the musculoskeletal system but also affects the emotional state. Physical activity influences physical and psychological well-being of people undergoing oncological treatment, in the course of which the most common psychiatric disorders are depression, substance use disorder, sleep disorders, fatigue syndrome, resulting in worsening of the quality of life. Difficulties in implementing physical exercise in cancer patients pose a challenge to treatment teams.
Field Epidemiology Training Program Response to COVID-19 During a Conflict: Experience From Yemen
Authors: Al Serouri Abdulwahed Abduljabar , Ghaleb Yasser Ahmed , Al Aghbari Labiba Anam , Al Amad Mohammad Abdullah , Alkohlani Abdulhakem Sharaf , Almoayed Khaled Abdullah , Jumaan Aisha Obad
Published: November 2021

COVID-19 pandemic has underscored the need for a well-trained public health workforce to save lives through timely outbreaks detection and response. In Yemen, a country that is entering its seventh year of a protracted war, the ongoing conflict severely limited the country's capacity to implement effective preparedness and response measures to outbreaks including COVID-19. There are growing concerns that the virus may be circulating within communities undetected and unmitigated especially as underreporting continues in some areas of the country due to a lack of testing facilities, delays in seeking treatment, stigma, difficulty accessing treatment centers, the perceived risks of seeking care or for political issues. The Yemen Field Epidemiology Training Program (FETP) was launched in 2011 to address the shortage of a skilled public health workforce, with the objective of strengthening capacity in field epidemiology. Thus, events of public health importance can be detected and investigated in a timely and effective manner. During the COVID-19 pandemic, the Yemen FETP's response has been instrumental through participating in country-level coordination, planning, monitoring, and developing guidelines/standard operating procedures and strengthening surveillance capacities, outbreak investigations, contact tracing, case management, infection prevention, and control, risk communication, and research. As the third wave is circulating with a steeper upward curve than the previous ones with possible new variants, the country will not be able to deal with a surge of cases as secondary care is extremely crippled. Since COVID-19 prevention and control are the only option available to reduce its grave impact on morbidity and mortality, health partners should support the Yemen FETP to strengthen the health system's response to future epidemics. One important lesson learned from the COVID-19 pandemic, especially in the Yemen context and applicable to developing and war-torn countries, is that access to outside experts becomes limited, therefore, it is crucial to invest in building national expertise to provide timely, cost-effective, and sustainable services that are culturally appropriate. It is also essential to build such expertise at the governorate and district levels, as they are normally the first respondents, and to provide them with the necessary tools for immediate response in order to overcome the disastrous delays.
Enhanced Recovery After Surgery in Patients With Hepatocellular Carcinoma Undergoing Laparoscopic Hepatectomy
Authors: Zhou Jiamin , He Xigan , Wang Miao , Zhao Yiming , Zhang Ning , Wang Longrong , Mao Anrong , Wang Lu
Published: November 2021

Objective: To compare the effectiveness and safety of enhanced recovery after surgery (ERAS) in patients with hepatocellular carcinoma (HCC) undergoing laparoscopic hepatectomy.

Methods: From September 2016 to June 2019, 282 patients were enrolled, and ERAS was implemented since March 2018. All indicators related to surgery, liver function, and postoperative outcomes were included in the analysis. Propensity score matching (PSM) identified 174 patients for further comparison.

Results: After PSM, the clinicopathological baselines were well-matched. The group showed significantly less intraoperative blood loss (100.00 [100.00–200.00] vs. 200.00 [100.00–300.00] ml, P = 0.001), fewer days before abdominal drainage tube removal (4.00 [3.00–4.00] days vs. 4.00 [3.00–5.00] days, P = 0.023), shorter hospital stay after surgery (6.00 [5.00–6.00] days vs. 6.00 [6.00–7.00] days, P < 0.001), and reduced postoperative morbidity (18.39 vs. 34.48%, P = 0.026). The proportion of patients with a pain score ≥ 4 was significantly lower in the ERAS group within the first 2 days after surgery (1.15 vs. 13.79% and 8.05 vs. 26.44%, P = 0.002 and P = 0.001, respectively). Pringle maneuver was performed more frequently in the ERAS group (70.11 vs. 18.39%, P < 0.001), and a significantly higher postoperative alanine aminotransferase level was also observed (183.40 [122.85–253.70] vs. 136.20 [82.93–263.40] U/l, P = 0.026). The 2-year recurrence-free survival was similar between the two groups (72 vs. 71%, P = 0.946).

Conclusions: ERAS programs are feasible and safe and do not influence mid-term recurrence in HCC patients undergoing laparoscopic hepatectomy.
Liver Bleeding Due to HELLP Syndrome Treated With Embolization and Liver Transplantation: A Case Report and Review of the Literature
Authors: Messina Valentina , Dondossola Daniele , Paleari Maria Chiara , Fornoni Gianluca , Tubiolo Daniela , Vergani Patrizia , Rona Roberto , Rossi Giorgio
Published: November 2021

Background: Liver bleeding secondary to haemolysis, elevated liver enzymes, and low platelet count (HELLP) syndrome is uncommon, but a life-threatening peripartum condition that needs a prompt multidisciplinary approach.

Case Presentation: In this study, we presented a case of 28-year-old pregnant woman, who was presented to the obstetrics department with signs of preeclampsia and foetal growth restriction. An emergency caesarean section was performed, and the patient developed a HELLP syndrome complicated by spontaneous liver rupture. After radiological and surgical procedures, liver failure became evident and liver transplantation was successfully performed. The patient and her daughter are now alive.

Conclusions: Despite the rarity of this disease, liver complications due to HELLP syndrome must be properly diagnosed and treated given the gravity of the possible evolution in young women. After diagnosis, the patients must be treated in specialised centres with gynaecological, liver surgery, and transplant skills.
A Review of Per- and Polyfluorinated Alkyl Substance Impairment of Reproduction
Authors: Chambers Weston S. , Hopkins Jaida G. , Richards Sean M.
Published: November 2021

In this review article, we compiled peer-reviewed literature describing PFAS exposure and reproductive effects in animals and humans. The aim was to compare environmental occurrence and effects of the most prominent long-chain PFAS compounds and their short-chain replacements. Long-chain PFAS compounds are known to persist in the environment due to their chemical stability, and also known to bioaccumulate; hence, these compounds are being replaced globally. Indeed, PFOA and PFOS are considered long-chain “forever pollutants,” and thus the potential reproductive risk may continue for decades. Much less is known about their short-chain replacements despite the fact that they becoming more widespread in the environment. Short-chain PFAS are generally less bioaccumulative than long-chain, but they are more mobile and persistent in aquatic ecosystems. The three most prominent of these are commonly referred to as GenX, ADONA and F53B. The short-chain PFAS have similar physical and chemical properties as their predecessors; however, because they are relatively new, much less is known about the potential to disrupt reproduction. Indeed, high-quality epidemiological studies are needed to determine associations between short-chain PFAS exposure and effects on reproductive health. However, epidemiological evidence is mounting that long-chain PFAS exposure is associated with reproductive effects (i.e., decrease in fertility, reduced fetal growth and birth weight, pregnancy-induced hypertension and preeclampsia, thyroid hormone disruption during pregnancy, and preterm birth). Evidence from animal models and human cell lines indicates that short-chain PFAS similarly affect reproductive endpoints; however, epidemiological studies are scarce and inconsistent. Although short-chain PFAS have been quantified in drinking water and sediment worldwide, most of these studies did not focus on quantitation of GenX, ADONA, and F53B. There are also many other short-chain PFAS byproducts of manufacturing that have yet to be identified and studied. When sum total concentration of long- and short-chain PFAS are considered, the concentration rises by an order or magnitude or greater, as will the risk of exposure and subsequent reproductive effects.
Design, 3D FEM Simulation and Prototyping of a Permanent Magnet Spherical Motor
Authors: Gündoğar Umut Yusuf , Zorlu Partal Sibel
Published: November 2021

In recent years, large tilt angles, uniform magnetic flux distributions, strong forces, and large torques for motors have increasingly become important for robotics, biomedical, and automotive applications that have multi-degrees of freedom (MDOFs) motion. Generally, one-degree of-freedom motors are applied in MDOF motion. These situations cause the systems to have very complex and large structures. In order to address these issues, a 2-DOF surface permanent magnet spherical motor with a new mechanical design for the movement of the rotor with a large tilt angle of ±45° was designed, simulated, produced and tested in this paper. The motor consisted of a 4-pole permanent magnet rotor and a 3-block stator with 18 coils. In this study, the mechanical structure of the proposed spherical permanent magnet motor surrounded the rotor with two moving parts to move at a large tilt angle of ±45° without using any mechanical components such as spherical bearings, joint bearings, and bearing covers. Thus, the tilt angle, force, and torque values of the proposed motor have been improved according to MDOF motion motors using spherical bearings, bearing covers, or joint bearings in their mechanical structures in the literature. Ansys Maxwell software was used for the design and simulation of the motor. Three-dimensional (3D) finite element method (FEM) analysis and experimental studies were carried out on the force, torque, and magnetic flux density distribution of the motor. Then, simulation results and experimental results were compared to validate the 3D FEM simulations results.
Genome-Wide Association Study for Detecting Salt-Tolerance Loci and Candidate Genes in Rice
Authors: Lar San Mar , Seo Jeonghwan , Jang Seong-Gyu , Zhang Hongjia , Lee Ah-Rim , Cao Fang-Yuan , Lee Ja-Hong , Kim Na-Eun , Lee Yoonjung , Park Yong-Jin , Lee Joohyun , Kwon Soon-Wook
Published: November 2021

Salinity is one of the major constraints causing soil problems and is considered a limitation to increased rice production in rice-growing countries. This genome-wide association study (GWAS) experiment was conducted to understand the genetic basis of salt tolerance at the seedling stage in Korean rice. After 10 days of salt stress treatment, salt tolerance was evaluated with a standard evaluation system using a visual salt injury score. With 191 Korean landrace accessions and their genotypes, including 266,040 single-nucleotide polymorphisms (SNPs), using a KNU Axiom Oryza 580K Genotyping Array, GWAS was conducted to detect three QTLs with significant SNPs with a −log10(P) threshold of ≥3.66. The QTL of qSIS2, showed −log10(P) = 3.80 and the lead SNP explained 7.87% of total phenotypic variation. The QTL of qSIS4, showed −log10(P) = 4.05 and the lead SNP explained 10.53% of total phenotypic variation. The QTL of qSIS8 showed −log10(P) = 3.78 and the lead SNP explained 7.83% of total phenotypic variation. Among the annotated genes located in these three QTL regions, five genes were selected as candidates (Os04g0481600, Os04g0485300, Os04g0493000, Os04g0493300, and Os08g0390200) for salt tolerance in rice seedlings based on the gene expression database and their previously known functions.
Effects of NaCl on Hydroponic Cultivation of Reichardia picroides (L.) Roth
Authors: Maggini Rita , Benvenuti Stefano , Leoni Federico , Incrocci Luca , Pardossi Alberto
Published: November 2021

Wild edible plant species are often more tolerant to salinity than many crop plants. Considering the salinization of irrigation water that is progressively affecting the Mediterranean region and the market demand for new plant foods, the cultivation of wild edible species could represent a valid alternative to conventional vegetable crops. In this study, Reichardia picroides (L.) Roth, a widespread spontaneous herb of ethnobotanical tradition, was grown for four or six weeks under a greenhouse in a floating system for the production of baby leaves. In order to improve the nutraceutical quality of the tissues, the plants were exposed to the following NaCl concentrations in the nutrient solution: 1.7 (control), 25, 50, and 100 mM. The results showed that a 4-week growing period in a floating system with 50 mM NaCl in the nutrient solution increased the content of bioactive molecules without affecting the fresh yield. After six weeks of cultivation, despite a decrease in biomass production as compared with the control, the leaves of salt-treated plants contained higher levels of bioactive molecules along with lower amounts of nitrate ion.
Plum Ripeness Analysis in Real Environments Using Deep Learning with Convolutional Neural Networks
Authors: Miragaia Rolando , Chávez Francisco , Díaz Josefa , Vivas Antonio , Prieto Maria Henar , Moñino Maria José
Published: November 2021

Digitization and technological transformation in agriculture is no longer something of the future, but of the present. Many crops are being managed by using sophisticated sensors that allow farmers to know the status of their crops at all times. This modernization of crops also allows for better quality harvests as well as significant cost savings. In this study, we present a tool based on Deep Learning that allows us to analyse different varieties of plums using image analysis to identify the variety and its ripeness status. The novelty of the system is the conditions in which the designed algorithm can work. An uncontrolled photographic acquisition method has been implemented. The user can take a photograph with any device, smartphone, camera, etc., directly in the field, regardless of light conditions, focus, etc. The robustness of the system presented allows us to differentiate, with 92.83% effectiveness, three varieties of plums through images taken directly in the field and values above 94% when the ripening stage of each variety is analyzed independently. We have worked with three varieties of plums, Red Beaut, Black Diamond and Angeleno, with different ripening cycles. This has allowed us to obtain a robust classification system that will allow users to differentiate between these varieties and subsequently determine the ripening stage of the particular variety.
Comparison and Characterization of Oxidation Resistance and Carbohydrate Content in Cd-Tolerant and -Sensitive Kentucky Bluegrass under Cd Stress
Authors: Wang Yong , Cui Ting , Niu Kuiju , Ma Huiling
Published: November 2021

Kentucky bluegrass (Poa pratensis L.), a turf grass species that is hypertolerant of cadmium (Cd), is a potential phytoremediation material for soil polluted with Cd. However, the mechanism of Cd phytotoxicity in Kentucky bluegrass is unclear. Here, we compared the phenotype, induction of oxidative stress, and structural and non-structural carbohydrate contents between a Cd-tolerant genotype (‘Midnight’, M) and Cd-sensitive genotype (‘Rugby’, R). The results showed that both genotypes accumulated more Cd in the roots, whereas the R genotype distributed more Cd into the leaves compared with the M genotype. In both genotypes, Cd inhibited the length and fresh weight of the leaves and roots; increased the peroxidase (POD) activity but inhibited ascorbate peroxidase (APX) and catalase (CAT) activity; and increased the superoxide radical (O2−), hydrogen peroxide (H2O2), and malondialdehyde (MDA) contents. However, the M genotype exhibited lower root length inhibition, and the H2O2 and MDA contents confirmed that the M genotype had increased Cd accumulation and resistance, while the R genotype exhibited a better distribution of Cd. Moreover, Cd stress significantly increased the soluble sugar, trehalose, and sucrose contents of both genotypes. Pectin, lignin, and cellulose were significantly increased to prevent the entry of Cd into the roots. The Cd-induced growth inhibition and physiological responses in Kentucky bluegrass were preliminarily explored herein, with the chelation of pectin, lignification, and antioxidant response being possible contributors to Cd detoxification in Kentucky bluegrass. In addition, the Cd-induced increase in trehalose, sucrose, and soluble sugar contents might play a pivotal role in the defense against Cd stress in Kentucky bluegrass.
Effects of Temperament on the Reproduction of Beef Cattle
Authors: Brandão Alice Poggi , Cooke Reinaldo Fernandes
Published: November 2021

Temperament is often defined as the behavioral expression of animals in response to human interaction. Cattle temperament can be evaluated using an association of chute score and exit velocity, with cattle then classified as adequate or excitable temperament. To assess the impacts of temperament on various beef systems, these evaluation criteria were associated with productive and reproductive parameters of Bos taurus and B. indicus-influenced cattle. Consistently across studies, excitable cattle had greater plasma cortisol compared to animals with adequate temperament. Studies also reported that excitable beef females have poorer reproductive performance compared to calmer cohorts, including reduced annual pregnancy rates, decreased calving rate, weaning rate, and kg of calf weaned/cow exposed to breeding. Acclimating B. indicus × B. taurus or B. taurus heifers to human handling improved behavioral expression of temperament and hastened puberty attainment. However, similar benefits were not observed when mature cows were acclimated to human handling. Collectively, temperament of beef females measured via behavioral responses upon human handling impacts their reproductive and productive responses independent of breed type, and should be considered for optimal beef cattle production.
Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil
Authors: Antezana Pablo Edmundo , Municoy Sofia , Pérez Claudio Javier , Desimone Martin Federico
Published: November 2021

Wounds represent a major healthcare problem especially in hospital-associated infections where multi-drug resistant strains are often involved. Nowadays, biomaterials with therapeutic molecules play an active role in wound healing and infection prevention. In this work, the development of collagen hydrogels loaded with silver nanoparticles and Cannabis sativa oil extract is described. The presence of the silver nanoparticles gives interesting feature to the biomaterial such as improved mechanical properties or resistance to collagenase degradation but most important is the long-lasting antimicrobial effect. Cannabis sativa oil, which is known for its anti-inflammatory and analgesic effects, possesses antioxidant activity and successfully improved the biocompatibility and also enhances the antimicrobial activity of the nanocomposite. Altogether, these results suggest that this novel nanocomposite biomaterial is a promising alternative to common treatments of wound infections and wound healing.