bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Methanol Addition on the Combustion Process of the Methanol/Diesel Dual-Fuel Based on an Optical Engine
Authors: Liu Jinping , Guo Guangzhao , Wei Mingrui
Published: December 2023

The combustion process of traditional diesel engines is mainly determined by the injection timing of diesel. There is a trade-off relationship between the soot and NOx (nitrogen oxides) during this combustion process, making it difficult to reduce these two emissions simultaneously. The use of methanol can not only solve the above problem, but also replace some fossil fuels. However, the effects of methanol injection into the intake duct on the flame propagation in diesel/methanol dual-fuel engines is not yet clear, and there is relatively little research on it. The effects of methanol addition on the combustion process of diesel/methanol dual fuel (DMDF) were achieved based on a modified optical engine in this paper. One injector is installed on the intake inlet to inject methanol, and the other injector is installed in the cylinder to inject diesel in two stages before the top dead center of compression. There are three tests conducted separately in this paper. Firstly, the effects of the methanol ratio (40%, 50%, 60%, and 70%) on the combustion process are investigated, with the total heat remaining unchanged. Secondly, the effects of the pre-injection mass of diesel (20%, 30%, 40%, and 50%) on the combustion process are investigated, which keeps the total diesel mass unchanged. Finally, the effects of the total mass of diesel on the combustion process are investigated while maintaining the mass of methanol unchanged. The dual-fuel combustion process is recorded by a high-speed camera. A combustion analyzer and other equipment were used to analyze the combustion. The results showed that CA10 is delayed, the pressure and the heat release rate (HRR) are reduced, and the number of pixels of the KL factor (KL) decreases significantly with the increasing methanol ratio. CA10 and CA50 are advanced, the pressure and HRR decrease, and the KL increases when the mass of pre-injected diesel increases. CA10 and CA50 are advanced, respectively, and CA90 is postponed due to the increase in diesel mass. The pressure and HRR increase, and the KL increases when the total mass of diesel increases.
Studies on Engine Oil Degradation Characteristics in a Field Test with Passenger Cars
Authors: Smigins Ruslans , Amatnieks Karlis , Birkavs Aivars , Górski Krzysztof , Kryshtopa Sviatoslav
Published: December 2023

Nowadays, a car’s engine oil change interval is an essential factor in reducing wear. The correct choice depends on various factors. This study analyzes the changes in the composition of three different engine oils (0W30, 5W30, and 5W40) during the generally accepted oil change interval (15,000 km) in gasoline and diesel cars during the post-warranty period. Commercially available low-level biofuel blends (B7 and E10) were used to power test vehicles in a field test. Engine oil samples were taken every 3000 km for more detailed analysis and tested in an accredited laboratory. The contaminants in the engine oil were determined using several testing methods: spectrometric analysis, gas chromatography, etc. Studies have shown that all used cars have an increase in the number of iron particles, an increased concentration of silicon, and also an increase in the number of nickel particles above 12,000 km. Tests also showed a sharp drop of molybdenum anti-friction additives 4.5 times and a gradual increase in fuel concentration for the Opel Insignia over 12,000 km, but over 9000 km, a significant increase in the concentration of chromium particles. Based on this research results, it is preferable to choose a maintenance interval of no more than 12,000 km for cars during the post-warranty period. In this way, the intensity of engine wear can be reduced due to the loss of adequate protective properties of the engine oil.
Simulation of a Building with Hourly and Daily Varying Ventilation Flow: An Application of the Simulink S-Function
Authors: Michalak Piotr
Published: December 2023

This paper presents an application of the Simulink stvmgain S-function for the thermal modelling of a building zone based on the resistance–capacitance scheme of EN ISO 13790. That model in the form of the state-space matrix with time-varying elements was used in simulations of a building with hourly and, suggested in that standard, daily averaged ventilation airflow in five European cities. The following two ventilation schedules were used: occupancy-based; and wind-dependent. Comparative simulations were conducted in EnergyPlus. In general, the results obtained for the annual heating and cooling demand were better for hourly than daily averaged ventilation with an error below 10%. However, in several cases of cooling, the error was above 30%. When considering hourly indoor air temperatures, the proposed method provided very good results with MAE of up to 0.52 °C and 0.46 °C, RMSE < 0.69 °C and 0.62 °C, and CV(RMSE) < 3.09% and 2.75% for the daily averaged and hourly ventilation flow, respectively. For wind-driven ventilation, the temperatures were as follows: MAE < 0.49 °C and 0.48 °C; RMSE < 0.69 °C and 0.68 °C; and CV(RMSE) < 3.01% and 2.97%.
Carbon Accumulation and the Possibility of Carbon Losses by Vertical Movement of Dissolved Organic Carbon in Western Siberian Peatlands
Authors: Zarov Evgeny A. , Lapshina Elena D. , Kuhlmann Iris , Schulze Ernst-Detlef
Published: December 2023

We studied the peat stratigraphy of the Mukhrino peatland, which is a typical ombrotrophic bog for the Middle Taiga zone of Western Siberia, to gain insights into its history, hydrology, and carbon fluxes. For the first time in Western Siberia, seven cores were collected from locations that were chosen to represent the typical present-day vegetation types, and this was performed for the dating of the separated dissolved (DOC) and particulate organic carbon (POC) fractions, which were determined using the Accelerator Mass Spectrometer (AMS) radiocarbon (14C) method. The oldest peat was found at the bottoms of an underlying lake (10,053 cal. year BP) and an ancient riverbed (10,989 cal. year BP). For the whole history of the peatland, the average peat accumulation rate was estimated to be 0.067 ± 0.018 cm yr−1 (ranging from 0.013 to 0.332 cm yr−1), and the carbon accumulation rate was 38.56 ± 12.21 g m−2 yr−1 (ranging from 28.46 to 57.91 g m−2 yr−1). There were clear age differences between the separated samples of the DOC and POC. The DOC was older than the POC in the uppermost 150 cm of the peat deposit and younger in the deeper layers. The difference in age increased with depth, reaching 2000–3000 years at the bottom of the peat deposit (depth of 430–530 cm). Following the consideration of a range of factors that could potentially cause the dating discrepancy, we hypothesised that the DOC continuously moves down into the mineral sediment beneath the peat, as an additional carbon flux that results in the mixing of younger and older carbon. On this basis, we estimated the apparent rate of the DOC’s downward movement and the associated rate of carbon loss. The first estimate of the average rate of the DOC’s downward movement in Western Siberia was 0.047 ± 0.019 cm yr−1, causing carbon loss in the range of 28–404 mg m−2 yr−1.
Unraveling the Influences of Sodium, Potassium, Magnesium, and Calcium on the Crystallization Behavior of Lactose
Authors: Wijayasinghe Rangani , Vasiljevic Todor , Chandrapala Jayani
Published: December 2023

The inability of lactose to properly crystallize due to the presence of high amounts of salts poses significant hurdles for its downstream processing with some dairy waste streams such as acid whey. This study aimed to investigate the physicochemical and thermal behaviors of lactose in the presence of cations commonly present in acid whey. A model-based study was conducted, utilizing various cations (Mg, Ca, K, and Na) at concentrations (8, 30, 38, and 22 mM, respectively) that are typically found in acid whey. The research experiments were conducted using a factorial design. The thermal analysis of concentrated solutions revealed augmentation in the enthalpy of water evaporation in the presence of individual cations and their combinations in comparison with pure lactose (698.4 J/g). The degree of enthalpy increased following the order of Na+ (918.6 J/g), K+ (936.6 J/g), Mg2+ (987.0 J/g), Ca2+ (993.2 J/g), and their mixture (1005.4 J/g). This resulted in a substantial crystal yield decline in the exactly reversed order to that of the enthalpy. The greatest decline was observed in the presence of the salt mixture (63%) followed by Ca (67%) compared with pure lactose (79%). The yield reduction was also inversely related to the solubility of lactose. The presence of divalent cations appeared to play a role in the isomerization of lactose molecules observed using DSC and XRD diffractograms according to the disappearance of peaks related to β lactose. The effect of salts on the crystallization of lactose was a combination of cation–lactose interactions, changes in the solubility of lactose, ion–dipole interactions between water and cations, and changes in the structure of water molecules. By deviating the composition of acid whey, the crystallization of lactose can be enhanced, leading to the improved downstream processing of acid whey.
Structural Characterization and Anti-Inflammatory Activity of Polysaccharides from Tremella fuciformis on Monosodium Urate-Stimulated RAW264.7 Macrophages
Authors: Deng Wei , Wu Li , Xiao Zheng , Li Yibin , Zheng Zhipeng , Chen Shouhui
Published: December 2023

The structural characteristics and anti-inflammatory activity of Tremella fuciformis polysaccharides (TFPs) were investigated. The study showed that TFPs were mainly composed of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose. TFPs significantly inhibited monosodium urate (MSU)-induced inflammation of RAW264.7 cells, as well as the secretion levels of TNF-α, IL-1β, and IL-18 cytokines. The concentrations of malondialdehyde and reactive oxygen species in RAW264.7 macrophages were reduced, but superoxide dismutase activity was increased. RNA-Seq technology was applied to explore the mechanisms of TFPs ameliorating MSU-induced inflammation of RAW264.7 macrophages. Results revealed that TFPs significantly reduce MSU-stimulated inflammatory damage in RAW 264.7 cells by inhibiting signaling pathways like the hypoxia inducible factor-1 (HIF-1) signaling pathway and erythroblastic oncogene B (ErbB) signaling pathway. This study provides a foundation for TFPs to be developed as novel anti-inflammatory drugs.
The Antioxidant Potential of Various Wheat Crusts Correlates with AGE Content Independently of Acrylamide
Authors: Wächter Kristin , Longin Carl Friedrich H. , Winterhalter Patrick R. , Bertsche Ute , Szabó Gábor , Simm Andreas
Published: December 2023

Epidemiological studies have indicated that the consumption of whole-grain products is associated with a reduced risk of cardiovascular diseases, type II diabetes, and cancer. In the case of bread, high amounts of antioxidants and advanced glycation end products (AGEs) are formed during baking by the Maillard reaction in the bread crust; however, the formation of potentially harmful compounds such as acrylamide also occurs. This study investigated the antioxidant responses of different soluble extracts from whole-grain wheat bread crust extracts (WBCEs) in the context of the asparagine, AGE, and acrylamide content. For that, we analyzed nine bread wheat cultivars grown at three different locations in Germany (Hohenheim, Eckartsweier, and Oberer Lindenhof). We determined the asparagine content in the flour of the 27 wheat cultivars and the acrylamide content in the crust, and measured the antioxidant potential using the induced expression of the antioxidant genes GCLM and HMOX1 in HeLa cells. Our study uncovered, for the first time, that the wheat crust’s antioxidant potential correlates with the AGE content, but not with the acrylamide content. Mass spectrometric analyses of WBCEs for identifying AGE-modified proteins relevant to the antioxidant potential were unsuccessful. However, we did identify the wheat cultivars with a high antioxidant potential while forming less acrylamide, such as Glaucus and Lear. Our findings indicate that the security of BCEs with antioxidative and cardioprotective potential can be improved by choosing the right wheat variety.
Food Components in Health Promotion and Disease Prevention
Authors: Chiavaroli Annalisa , Brunetti Luigi
Published: December 2023

In recent years, more plant-based sources of functional foods have been shown to be effective in preventing, reducing, and treating chronic inflammatory and metabolic diseases, and promoting health [...]
The Prebiotic Activity of a Novel Polysaccharide Extracted from Huangshui by Fecal Fermentation In Vitro
Authors: Li Mei , Su Jian , Wu Jihong , Zhao Dong , Huang Mingquan , Lu Yanping , Zheng Jia , Li Hehe
Published: December 2023

A novel polysaccharide, HSP80-2, with an average molecular weight of 13.8 kDa, was successfully isolated by the gradient ethanol precipitation (GEP) method from Huangshui (HS), the by-product of Chinese Baijiu. It was mainly composed of arabinose, xylose, and glucose with a molar ratio of 4.0:3.1:2.4, which was completely different from the previous reported HS polysaccharides (HSPs). Morphological observations indicated that HSP80-2 exhibited a smooth but uneven fragmented structure. Moreover, HSP80-2 exerted prebiotic activity evaluated by in vitro fermentation. Specifically, HSP80-2 was utilized by gut microbiota, and significantly regulated the composition and abundance of beneficial microbiota such as Phascolarctobacterium, Parabacteroides, and Bacteroides. Notably, KEGG pathway enrichment analysis illustrated that HSP80-2 enriched the pathways of amino sugar and nucleotide sugar metabolism (Ko00520), galactose metabolism (ko00052), and the citrate cycle (TCA cycle) (ko00020). Meanwhile, the contents of short-chain fatty acids (SCFAs) mainly including acetic acid, propionic acid, and butyric acid in the HSP80-2 group were remarkably increased, which was closely associated with the growth of Lachnoclostridium and Parabacteroides. These results showed that HSP80-2 might be used as a potential functional factor to promote human gut health, which further extended the high value utilization of HS.
Study on the Residence Time and Texture Prediction of Pea Protein Extrusion Based on Image Analysis
Authors: Wu Qi , Zhang Xun , Gao Fei , Wu Min
Published: December 2023

This paper initially involves three main processing parameters: screw speed, feeding speed, and initial material moisture content, exploring the RTD of materials inside the extruder barrel under varying parameters and clarifying the impact of parameter variations on RTD. Finally, machine vision technology was utilized to link extruded product images to texture features, and a texture prediction model based on image features was established using a Back Propagation (BP) neural network. Particle Swarm Optimization (PSO) and Genetic Algorithm (GA) were applied to optimize the BP neural network. The results indicate that the feeding speed has a stronger impact than the screw speed on the extrusion process, and an increase in the initial material moisture content tends to shorten the RTD. Specifically, an increase in screw speed results in a denser product structure, while higher feeding speeds lead to reduced pore size in the microstructure. As the initial material moisture content increased from 55% to 70%, the average residence time MRT decreased from 265.21 s to 166.62 s. Additionally, elevated moisture content causes a more porous microstructure. After optimizing the texture prediction model of extruded products through the application of Particle Swarm Optimization and Genetic Algorithm models, it was discovered that the Genetic Algorithm was more effective in reducing errors (p < 0.05) than the Particle Swarm Optimization algorithm. It was found that the Particle Swarm Optimization model exhibited better prediction performance. The results of the prediction indicated a significant association between the image features of the product and hardness, resilience, and chewiness, as corroborated by correlation coefficients of 0.93913, 0.94040, and 0.94724, respectively.
Huangqin Tea Total Flavonoids–Gut Microbiota Interactions: Based on Metabolome and Microbiome Analysis
Authors: Zheng Yaping , Yang Kailin , Shen Jie , Chen Xiangdong , He Chunnian , Xiao Peigen
Published: December 2023

Huangqin tea (HQT), a Non-Camellia Tea derived from the aerial parts of Scutellaria baicalensis, is widely used in the north of China. The intervention effects of HQT on intestinal inflammation and tumors have been found recently, but the active ingredient and mechanism of action remain unclear. This study aimed to investigate the interactions between the potential flavonoid active components and gut microbiota through culture experiments in vitro combined with HPLC-UV, UPLC-QTOF-MS, and 16S rDNA sequencing technology. The results showed that the HQT total flavonoids were mainly composed of isocarthamidin-7-O-β-D-glucuronide, carthamidin-7-O-β-D-glucuronide, scutellarin, and others, which interact closely with gut microbiota. After 48 h, the primary flavonoid glycosides transformed into corresponding aglycones with varying degrees of deglycosylation. The composition of the intestinal microbiota was changed significantly. The beneficial bacteria, such as Enterococcus and Parabacteroides, were promoted, while the harmful bacteria, such as Shigella, were inhibited. The functional prediction results have indicated notable regulatory effects exerted by total flavonoids and scutellarin on various pathways, including purine metabolism and aminoacyl-tRNA biosynthesis, among others, to play a role in the intervention of inflammation and tumor-related diseases. These findings provided valuable insights for further in-depth research and investigation of the active ingredients, metabolic processes, and mechanisms of HQT.
Activated Carbon-Incorporated Tragacanth Gum Hydrogel Biocomposite: A Promising Adsorbent for Crystal Violet Dye Removal from Aqueous Solutions
Authors: Thamer Badr M. , Al-aizari Faiz A. , Abdo Hany S.
Published: December 2023

Biomaterials-based adsorbents have emerged as a sustainable and promising solution for water purification, owing to their eco-friendly nature and remarkable adsorption capacities. In this study, a biocomposite hydrogel was prepared by the incorporation of activated carbon derived from pomegranate peels (PPAC) in tragacanth gum (TG). The hydrogel biocomposite (PPAC/TG) showed a porous structure, a negative surface charge at a pH of more than 4.9, and good stability in aqueous media. The adsorption properties of the PPAC/TG hydrogel biocomposite were assessed for the removal of crystal violet dye (CV) from aqueous solutions using a batch adsorption. The equilibrium adsorption data followed the Sips isotherm model, as supported by the calculated R2 (>0.99), r-χ2 (<64), and standard error values (<16). According to the Sips model, the maximum values of the adsorption capacity of PPAC/TG were 455.61, 470.86, and 477.37 mg/g at temperatures of 25, 30, and 35 °C, respectively. The adsorption kinetic of CV onto the PPAC/TG hydrogel biocomposite was well described by the pseudo-second-order model with R2 values more than 0.999 and r-χ2 values less than 12. Thermodynamic studies confirmed that the CV dye adsorption was spontaneous and endothermic. Furthermore, the prepared hydrogel exhibited excellent reusability, retaining its adsorption capacity even after being used more than five times. Overall, this study concludes that the prepared PPAC/TG exhibited a significant adsorption capacity for cationic dyes, indicating its potential as an effective and eco-friendly adsorbent for water treatment.
Whole Genome Analysis of SNV and Indel Polymorphism in Common Marmosets (Callithrix jacchus)
Authors: Harris R. Alan , Raveendran Muthuswamy , Warren Wes , LaDeana Hillier W. , Tomlinson Chad , Graves-Lindsay Tina , Green Richard E. , Schmidt Jenna K. , Colwell Julia C. , Makulec Allison T. , Cole Shelley A. , Cheeseman Ian H. , Ross Corinna N. , Capuano Saverio , Eichler Evan E. , Levine Jon E. , Rogers Jeffrey
Published: December 2023

The common marmoset (Callithrix jacchus) is one of the most widely used nonhuman primate models of human disease. Owing to limitations in sequencing technology, early genome assemblies of this species using short-read sequencing suffered from gaps. In addition, the genetic diversity of the species has not yet been adequately explored. Using long-read genome sequencing and expert annotation, we generated a high-quality genome resource creating a 2.898 Gb marmoset genome in which most of the euchromatin portion is assembled contiguously (contig N50 = 25.23 Mbp, scaffold N50 = 98.2 Mbp). We then performed whole genome sequencing on 84 marmosets sampling the genetic diversity from several marmoset research centers. We identified a total of 19.1 million single nucleotide variants (SNVs), of which 11.9 million can be reliably mapped to orthologous locations in the human genome. We also observed 2.8 million small insertion/deletion variants. This dataset includes an average of 5.4 million SNVs per marmoset individual and a total of 74,088 missense variants in protein-coding genes. Of the 4956 variants orthologous to human ClinVar SNVs (present in the same annotated gene and with the same functional consequence in marmoset and human), 27 have a clinical significance of pathogenic and/or likely pathogenic. This important marmoset genomic resource will help guide genetic analyses of natural variation, the discovery of spontaneous functional variation relevant to human disease models, and the development of genetically engineered marmoset disease models.
The Epidemiological Transition of Surgically Treated Proximal Hip Fractures in Austria over the Course of the Pandemic—Back to Normal or a New Normal?
Authors: Popp Domenik , Nia Arastoo , Silvaieh Sara , Sator Thomas , Tiefenboeck Thomas M. , Schmoelz Lukas , Babeluk Rita , Hajdu Stefan , Widhalm Harald K.
Published: December 2023

Background: The COVID-19 pandemic has had a significant impact on the treatment protocols of orthopedic and trauma departments, but its specific effect on the mortality of hip fracture patients due to possible delays in surgery remains uncertain. This study aimed to investigate whether the COVID-19 pandemic worsened the mortality of patients with hip fractures. Materials and methods: This study included 246 prospectively enrolled patients who suffered from hip fractures during the Austrian State of Emergency period between 1 March and 30 June 2020 and 2021 and were admitted to a tertiary care trauma center. This cohort was compared with a retrospective control group of 494 patients admitted for hip fractures during the same timeframe in 2017, 2018, and 2019. These groups were compared to a prospective recruited “post-COVID-19 collective consisting of the years 2022 and 2023 including 313 patients. Results: This study found a 22% reduction in admissions during the COVID-19 period compared to the pre-COVID period (p = 0.018), as well as significant changes in gender (p = 0.013) and place of accident (p = 0.049). No other changes in demographic variables were observed. The 30-day mortality rate was 14.67% in the pre-COVID period, compared to 15.18% during the COVID-19 period (p = 0.381). No differences were observed in surgical complication rates or in the relationship between comorbidity burden and survival. Conclusion: This study did not show a higher perioperative mortality rate due to COVID-19. However, under current circumstances, with potentially reduced surgical and hospital bed capacities, it is expected that this condition might require a high degree of resources in times when resources are potentially scarce, such as during an ongoing pandemic. Level of evidence: Level III.
Alleged Malpractice in Orthopedic Surgery in The Netherlands: Lessons Learned from Medical Disciplinary Jurisprudence
Authors: Harlianto Netanja I. , Harlianto Zaneta N.
Published: December 2023

Background: Orthopedic surgery is a specialty at risk for medical malpractice claims. We aimed to assess the frequency of alleged malpractice cases related to orthopedic surgery in the Netherlands from the last 15 years. Methods: We systematically searched the database of the Dutch Medical Disciplinary Court for verdicts related to orthopedic surgery between January 2009 and July 2023 and extracted case data and data on allegations and outcomes. Results: We identified 158 verdicts (mean of 10.5 per year), of which 151 (96%) were filed against specialists and 7 (4%) against residents. Cases were most frequently classified as incorrect treatment/diagnosis (n = 107, 67.7%). Cases were related to the subspecialties of knee (n = 34, 21.5%), hip (n = 31, 19.6%), ankle (n = 25, 15.8%), spine (n = 22, 13.9%), and shoulder (n = 19, 12.0%). A total of 32 cases (20.3%) were judged as partially founded and 9 (6%) as founded. The Dutch Medical Disciplinary Court imposed 28 warnings, 10 reprimands, and 3 temporary suspensions. A total of 68 appeals were submitted, of which 95% were rejected for filing patients. In three instances, unfounded verdicts were changed to two warnings and a reprimand. In four appeals by an orthopedic surgeon, a warning and reprimand were dismissed, and two reprimands were changed to warnings. Conclusions: The amount of malpractice cases against orthopedic surgeons in the Netherlands is relatively low. The cases in our study may improve our understanding of allegations against physicians and improve the quality of patient care.
Paediatric Emergency Asthma Presentations: Temporal Trends and Representations in Rural Australia
Authors: Terry Daniel , Peck Blake , Kloot Kate
Published: December 2023

Asthma is a key illness driving children to present to emergency departments, and although paediatric emergency asthma presentations have been examined, the temporal trends remain somewhat elusive. The aim is to highlight, describe, and model the temporal trends of emergency paediatric asthma presentations, using comprehensive hospital emergency presentation data. A retrospective cross-sectional study examined de-identified paediatric (0 to 14 years) emergency asthma presentation data over a three-year period. Data were obtained from nine healthcare facilities in Victoria, Australia. Episode-level data were collected through RAHDaR, a comprehensive emergency data register which includes missing data (35.0%) among rural health facilities not currently captured elsewhere. Monthly presentation rates demonstrate a significant difference in presentations between fall/autumn and spring, and males had higher presentation rates in February and June–August. Emergency presentations were more likely to occur Sunday–Tuesday, peaking in the time periods of 8–9 a.m., 11 a.m.–12 p.m., and 8–9 p.m. Significant differences were noted between all age groups. Examining previously unavailable rural data has highlighted patterns among emergency asthma presentations for children 0–14 years of age. Knowledge of these by season, month, and day of the week, in combination with time of day, offers scope for more focused workforce education and planning, and nuanced referral pathways, particularly in resource-limited settings.
Alexithymia in Adult Autism Clinic Service-Users: Relationships with Sensory Processing Differences and Mental Health
Authors: Josyfon Emily , Spain Debbie , Blackmore Charlotte , Murphy Declan , Oakley Bethany
Published: December 2023

Mental health difficulties commonly co-occur with autism, especially in autistic people accessing clinic services, impacting substantially on quality-of-life. Alexithymia (difficulty describing/identifying feelings) and sensory processing differences are prevalent traits in autism that have been associated with depression/anxiety in autistic community samples. However, it is important to better understand interrelationships between these traits in clinical populations to improve identification of service-user needs. In this study, 190 autistic adults (65.3% male), seen in a tertiary autism clinic, completed self-report measures of alexithymia (20-item Toronto Alexithymia Scale), sensory processing differences (Adolescent/Adult Sensory Profile) and depression/anxiety (Hospital Anxiety and Depression Scale). Multiple linear regression models and mediation analyses were used to examine associations between alexithymia, sensory processing differences, and depression/anxiety severity. Across the sample, 66.3% of individuals (N = 126) were classified as alexithymic (score ≥ 61). Total alexithymia and difficulty describing/identifying feelings were significantly associated with depression severity (β = 0.30–0.38, highest p < 0.002), and difficulty identifying feelings was significantly associated with anxiety severity (β = 0.36, p < 0.001). Sensory processing differences were also significantly associated with depression severity (β = 0.29, p = 0.002) and anxiety severity across all models (β = 0.34–0.48, highest p < 0.001) Finally, difficulty describing/identifying feelings partially mediated the relationships between sensory processing differences and both depression/anxiety severity. Overall, these results highlight that interventions adapted for and targeting emotional awareness and sensory-related uncertainty may improve mental health outcomes in autistic service-users.
Towards the Future of Public Health: Roadmapping Trends and Scenarios in the Post-COVID Healthcare Era
Authors: El-Warrak Leonardo , Nunes Mariano , Luna Gabriel , Barbosa Carlos Eduardo , Lyra Alan , Argôlo Matheus , Lima Yuri , Salazar Herbert , de Souza Jano Moreira
Published: December 2023

The COVID-19 pandemic, a transformative event in modern society, has disrupted routine, work, behavior, and human relationships. Organizations, amidst the chaos, have innovatively adapted to the evolving situation. However, many countries were unprepared for the magnitude of the challenge, revealing the fragility of health responses due to inadequate leadership, insufficient resources, and poor information system integration. Structural changes in health systems are imperative, particularly in leadership, governance, human resources, financing, information systems, technology, and health service provision. This research utilizes the Technological Roadmapping method to analyze the health sector, focusing on public health, drawing on articles from SCOPUS and PubMed databases, and creating a roadmap extending to 2050. The research presents three long-term scenarios based on the literature-derived roadmap and explores various alternatives, including integrated care, telemedicine, Big Data utilization, nanotechnology, and Big Tech’s AI services. The results underscore the anticipation of post-pandemic public health with high expectations, emphasizing the importance of integrating health history access, encouraging self-care, and leveraging technology for streamlined treatment. Practical implications include insights for decision makers and stakeholders to inform strategic planning and adapt to evolving industry demands, recognizing the significance of preventive services and the humanizing potential of technology.
Xylella fastidiosa, Possible New Threat to Chestnut (Castanea sativa Mill.) in Italy
Authors: Greco Davide , Sabella Erika , Carluccio Giambattista , Delle Donne Angelo Giovanni , De Bellis Luigi , Luvisi Andrea
Published: December 2023

This is the first worldwide report of X. fastidiosa (Xf) subsp. pauca on Castanea sativa and the first characterization of Xf infection on this species. Plants located in three sites (in a long-term affected area in Apulia) were monitored for symptoms and bacterial concentrations in spring and summer, while microscopic analyses were performed to evaluate the pathogen distribution in the xylem vessels. All chestnut plants appeared asymptomatic but the Xf subsp. pauca strain “De Donno” was present at low concentrations (1.14 × 104 and 1.56 × 103 cfu mL−1 in April and June) and with a low incidence (respectively, 38% and 30%). The FISH-CLSM (Fluorescent In Situ Hybridization—Confocal Laser Scanning Microscope) analysis showed evident Xf occlusions but in a low percentage (9.2 ± 3.4%); these data can explain the lack of symptoms on the canopy. Furthermore, through a bibliographic analysis it emerged that Philaenus spumarius and Neophilaenus campestris, two Xf vectors present in Europe, are suckling feeding insects on chestnut trees and could be involved in the bacterial transmission to this species. Asymptomatic Xf host species can play a considerable role in new outbreak emergence or in the expansion of existing ones. So, it is essential to identify them to plan more effective monitoring activities.
Exploring Efficient Methods for Using Multiple Spectral Reflectance Indices to Establish a Prediction Model for Early Drought Stress Detection in Greenhouse Tomato
Authors: Fang Shih-Lun , Cheng Yu-Jung , Tu Yuan-Kai , Yao Min-Hwi , Kuo Bo-Jein
Published: December 2023

Early detection of drought stress in greenhouse tomato (Solanum lycopersicum) is an important issue. Real-time and nondestructive assessment of plant water status is possible by spectroscopy. However, spectral data often suffer from the problems of collinearity, class imbalance, and class overlap, which require some effective strategies to overcome. This study used a spectroscopic dataset on the tomato (cv. ‘Rosada’) vegetative stage and calculated ten spectral reflectance indices (SRIs) to develop an early drought detection model for greenhouse tomatoes. In addition, this study applied the random forest (RF) algorithm and two resampling techniques to explore efficient methods for analyzing multiple SRI data. It was found that the use of the RF algorithm to build a prediction model could overcome collinearity. Moreover, the synthetic minority oversampling technique could improve the model performance when the data were imbalanced. For class overlap in high-dimensional data, this study suggested that two to three important predictors can be screened out, and it then used a scatter plot to decide whether the class overlap should be addressed. Finally, this study proposed an RF model for detecting early drought stress based on three SRIs, namely, RNDVI, SPRI, and SR2, which only needs six spectral wavebands (i.e., 510, 560, 680, 705, 750, and 900 nm) to achieve more than 85% accuracy. This model can be a useful and cost-effective tool for precise irrigation in greenhouse tomato production, and its sensor prototype can be developed and tested in different situations in the future.
Metabolomics (Non-Targeted) of Induced Type 2 Diabetic Sprague Dawley Rats Comorbid with a Tissue-Dwelling Nematode Parasite
Authors: Ndlovu Innocent Siyanda , Tshilwane Selaelo Ivy , Ngcamphalala Philile Ignecious , Vosloo Andre’ , Chaisi Mamohale , Mukaratirwa Samson
Published: December 2023

Type 2 diabetes is a non-communicable metabolic syndrome that is characterized by the dysfunction of pancreatic β-cells and insulin resistance. Both animal and human studies have been conducted, demonstrating that helminth infections are associated with a decreased prevalence of type 2 diabetes mellitus (T2DM). However, there is a paucity of information on the impact that helminths have on the metabolome of the host and how the infection ameliorates T2DM or its progression. Therefore, this study aimed at using a non-targeted metabolomics approach to systematically identify differentiating metabolites from serum samples of T2DM-induced Sprague Dawley (SD) rats infected with a tissue-dwelling nematode, Trichinella zimbabwensis, and determine the metabolic pathways impacted during comorbidity. Forty-five male SD rats with a body weight between 160 g and 180 g were used, and these were randomly selected into control (non-diabetic and not infected with T. zimbabwensis) (n = 15) and T2DM rats infected with T. zimbabwensis (TzDM) (n = 30). The results showed metabolic separation between the two groups, where d-mannitol, d-fructose, and glucose were upregulated in the TzDM group, when compared to the control group. L-tyrosine, glycine, diglycerol, L-lysine, and L-hydroxyproline were downregulated in the TzDM group when compared to the control group. Metabolic pathways which were highly impacted in the TzDM group include biotin metabolism, carnitine synthesis, and lactose degradation. We conclude from our study that infecting T2DM rats with a tissue-dwelling nematode, T. zimbabwensis, causes a shift in the metabolome, causing changes in different metabolic pathways. Additionally, the infection showed the potential to regulate or improve diabetes complications by causing a decrease in the amino acid concentration that results in metabolic syndrome.
Mitochondrial DNA Variants at Low-Level Heteroplasmy and Decreased Copy Numbers in Chronic Kidney Disease (CKD) Tissues with Kidney Cancer
Authors: Kanazashi Yuki , Maejima Kazuhiro , Johnson Todd A. , Sasagawa Shota , Jikuya Ryosuke , Hasumi Hisashi , Matsumoto Naomichi , Maekawa Shigekatsu , Obara Wataru , Nakagawa Hidewaki
Published: December 2023

The human mitochondrial genome (mtDNA) is a circular DNA molecule with a length of 16.6 kb, which contains a total of 37 genes. Somatic mtDNA mutations accumulate with age and environmental exposure, and some types of mtDNA variants may play a role in carcinogenesis. Recent studies observed mtDNA variants not only in kidney tumors but also in adjacent kidney tissues, and mtDNA dysfunction results in kidney injury, including chronic kidney disease (CKD). To investigate whether a relationship exists between heteroplasmic mtDNA variants and kidney function, we performed ultra-deep sequencing (30,000×) based on long-range PCR of DNA from 77 non-tumor kidney tissues of kidney cancer patients with CKD (stages G1 to G5). In total, this analysis detected 697 single-nucleotide variants (SNVs) and 504 indels as heteroplasmic (0.5% ≤ variant allele frequency (VAF) < 95%), and the total number of detected SNVs/indels did not differ between CKD stages. However, the number of deleterious low-level heteroplasmic variants (pathogenic missense, nonsense, frameshift and tRNA) significantly increased with CKD progression (p < 0.01). In addition, mtDNA copy numbers (mtDNA-CNs) decreased with CKD progression (p < 0.001). This study demonstrates that mtDNA damage, which affects mitochondrial genes, may be involved in reductions in mitochondrial mass and associated with CKD progression and kidney dysfunction.
IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish
Authors: Zhai Wenya , Wang Zhensheng , Ye Canxun , Ke Lan , Wang Huanling , Liu Hong
Published: December 2023

Interleukin-6 (IL-6), a pleiotropic cytokine, plays a crucial role in acute stress induced by bacterial infection and is strongly associated with reactive oxygen species (ROS) production. However, the role of IL-6 in the liver of fish after Aeromonas hydrophila infection remains unclear. Therefore, this study constructed a zebrafish (Danio rerio) il-6 knockout line by CRISPR/Cas9 to investigate the function of IL-6 in the liver post bacterial infection. After infection with A. hydrophila, pathological observation showed that il-6−/− zebrafish exhibited milder liver damage than wild-type (WT) zebrafish. Moreover, liver transcriptome sequencing revealed that 2432 genes were significantly up-regulated and 1706 genes were significantly down-regulated in il-6−/− fish compared with WT fish after A. hydrophila infection. Further, gene ontology (GO) analysis showed that differentially expressed genes (DEGs) were significantly enriched in redox-related terms, including oxidoreductase activity, copper ion transport, etc. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in pathways such as the PPAR signaling pathway, suggesting that il-6 mutation has a significant effect on redox processes in the liver after A. hydrophila infection. Additionally, il-6−/− zebrafish exhibited lower malondialdehyde (MDA) levels and higher superoxide dismutase (SOD) activities in the liver compared with WT zebrafish following A. hydrophila infection, indicating that IL-6 deficiency mitigates oxidative stress induced by A. hydrophila infection in the liver. These findings provide a basis for further studies on the role of IL-6 in regulating oxidative stress in response to bacterial infections.
The Role of Neutrophils in ANCA-Associated Vasculitis: The Pathogenic Role and Diagnostic Utility of Autoantibodies
Authors: Walulik Agata , Łysak Kinga , Błaszkiewicz Michał , Górecki Ignacy , Gomułka Krzysztof
Published: December 2023

Recent years have brought progress in understanding the role of the neutrophil, dispelling the dogma of homogeneous cells mainly involved in the prime defence against pathogens, shedding light on their pathogenic role in inflammatory diseases and on the importance of antineutrophil-cytoplasmic antibodies’ pathogenic role in ANCA-associated vasculitides vasculitis (AAV). Myeloperoxidase (MPO) and proteinase 3 (PR3) expressed in neutrophil granulocytes are the most common targets for ANCAs and contribute to the formation of MPO-ANCAs and PR3-ANCAs which, released to the bloodstream, become an excellent diagnostic tool for AAV. In this study, we focus on increasing the clinical and experimental evidence that supports the pathogenic role of ANCAs in AAV. Additionally, we discuss the diagnostic utility of ANCAs for disease activity and prognosis in AAV. Understanding the central role of ANCAs in AAV is crucial for advancing our knowledge of these complex disorders and developing targeted therapeutic strategies in the era of personalized medicine.
Seawater Accelerated the Aging of Polystyrene and Enhanced Its Toxic Effects on Caenorhabditis elegans
Authors: Zhou Tong , Wu Jiajie , Liu Yun , Xu An
Published: December 2023

Microplastics (MPs) are emerging pollutants and pose a significant threat to marine ecosystems. Although previous studies have documented the mechanisms and toxic effects of aging MPs in various environments, the impact of the marine environment on MPs remains unclear. In the present study, the aging process of polystyrene (PS) in seawater was simulated and the changes in its physicochemical properties were investigated. Our results showed that the surface of the PS eroded in the seawater, which was accompanied by the release of aged MPs with a smaller size. In situ optical photothermal infrared microspectroscopy revealed that the mechanism of PS aging was related to the opening of the carbonyl group and breaking of the bond between carbon and benzene removal. To verify the toxic effects of aged PS, Caenorhabditis elegans was exposed to PS. Aged PS resulted in a greater reduction in locomotion, vitality, and reproduction than virgin PS. Mechanistically, aged PS led to oxidative stress, high glutathione s-transferase activity, and high total glutathione in worms. Together, our findings provided novel information regarding the accelerated aging of PS in seawater and the increased toxicity of aged PS, which could improve our understanding of MPs’ ecotoxicity in the marine environment.
Loss of Cdc42 in Exocrine Acini Decreases Saliva Secretion but Increases Tear Secretion—A Potential Model of Exocrine Gland Senescence
Authors: Nagase Haruna , Shitara Akiko , Ohno Yuta , Satoh Keitaro , Kashimata Masanori
Published: December 2023

Cdc42 is a small GTPase essential for the cell cycle, morphogenesis, and cell adhesion, and it is involved in the polarity of epithelial cells. However, the functional roles of Cdc42 in exocrine glands, such as the maintenance of acini and water secretion, are not yet well understood. In this study, we generated acinar-cell-specific Cdc42 conditional knockout (Cdc42cKO) mice to assess their maintenance of acinar cells and physiological functions in the salivary glands (SGs) and lacrimal glands (LGs). Our data revealed that the loss of Cdc42 altered the luminal structures to bulging structures and induced acinar cell apoptosis in both the parotid glands (PGs) and LGs of Cdc42cKO mice. Interestingly, saliva secretion in response to pilocarpine stimulation was decreased in the Cdc42cKO group, whereas tear secretion was increased. Consistent with the water secretion results, protein expression of the water channel AQP5 in acinar cells was also decreased in the PGs but conversely increased in the LGs. Moreover, the changes that increased AQP5 expression in LGs occurred in the acinar cells rather than the duct cells. The present study demonstrates that Cdc42 is involved in the structural and survival maintenance of acinar cells in SGs and LGs. On the other hand, depletion of Cdc42 caused the opposite physiological phenomena between PGs and LGs.
The Effects of Periodontal Treatment on Rheumatoid Arthritis and of Anti-Rheumatic Drugs on Periodontitis: A Systematic Review
Authors: Inchingolo Francesco , Inchingolo Angelo Michele , Avantario Pasquale , Settanni Vito , Fatone Maria Celeste , Piras Fabio , Di Venere Daniela , Inchingolo Alessio Danilo , Palermo Andrea , Dipalma Gianna
Published: December 2023

Rheumatoid arthritis (RA) and periodontitis are chronic inflammatory diseases that widely spread and share the same patterns of pro-inflammatory cytokines. This systematic review aims to evaluate the effects of non-surgical periodontal treatment (NSPT) on RA and, conversely, the impact of disease-modifying anti-rheumatic drugs (DMARDs) on periodontitis. PubMed, Embase, and Web of Science were searched using the MESH terms “periodontitis” and “rheumatoid arthritis” from January 2012 to September 2023. A total of 49 articles was included in the final analysis, 10 of which were randomized controlled trials. A total of 31 records concerns the effect of NSPT on parameters of RA disease activity, including a 28-joint disease activity score, anti-citrullinated protein antibodies, rheumatoid factor, C reactive protein, erythrocyte sedimentation rate, pro-inflammatory cytokines and acute phase proteins in serum, saliva, gingival crevicular fluid, and synovial fluid. A total of 18 articles investigated the effect of DMARDs on periodontal indexes and on specific cytokine levels. A quality assessment and risk-of-bias of the studies were also performed. Despite some conflicting results, there is evidence that RA patients and periodontitis patients benefit from NSPT and DMARDs, respectively. The limitations of the studies examined are the small samples and the short follow-up (usually 6 months). Further research is mandatory to evaluate if screening and treatment of periodontitis should be performed systematically in RA patients, and if the administration of DMARDs is useful in reducing the production of cytokines in the periodontium.