bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive detection of CRLF2 alterations in acute lymphoblastic leukemia: a rapid and accurate novel approach
Authors: Gil José Vicente , Miralles Alberto , de las Heras Sandra , Such Esperanza , Avetisyan Gayane , Díaz-González Álvaro , Santiago Marta , Fuentes Carolina , Fernández José María , Lloret Pilar , Navarro Irene , Montesinos Pau , Llop Marta , Barragán Eva
Published: February 2024

Introduction: Acute lymphoblastic leukemia (ALL) is a prevalent childhood cancer with high cure rate, but poses a significant medical challenge in adults and relapsed patients. Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL) is a high-risk subtype, with approximately half of cases characterized by CRLF2 overexpression and frequent concomitant IKZF1 deletions.

Methods: To address the need for efficient, rapid, and cost-effective detection of CRLF2 alterations, we developed a novel RT-qPCR technique combining SYBR Green and highresolution melting analysis on a single plate.

Results: The method successfully identified CRLF2 expression, P2RY8::CRLF2 fusions, and CRLF2 and JAK2 variants, achieving a 100% sensitivity and specificity. Application of this method across 61 samples revealed that 24.59% exhibited CRLF2 overexpression, predominantly driven by IGH::CRLF2 (73.33%). High Resolution Melting analysis unveiled concurrent CRLF2 or JAK2 variants in 8.19% of samples, as well as a dynamic nature of CRLF2 alterations during disease progression.

Discussion: Overall, this approach provides an accurate identification of CRLF2 alterations, enabling improved diagnostic and facilitating therapeutic decision-making.
Optimization of sensitivity and specificity of a biomarker-based blood test (LVOCheck-Opti): A protocol for a multicenter prospective observational study of patients suspected of having a stroke
Authors: Kaffes Maximilian , Bondi Fulvio , Geisler Frederik , Grittner Ulrike , Haacke Lisa , Ihl Thomas , Lorenz Maren , Schehadat Marc S. , Schwabauer Eugen , Wendt Matthias , Zuber Martina , Kübler-Weller Dorothee , Lorenz-Meyer Irina , Sanchez Jean-Charles , Montaner Joan , Audebert Heinrich J. , Weber Joachim E.
Published: February 2024

Introduction

Acute ischemic stroke (AIS) is a time-critical medical emergency. For patients with large-vessel occlusions (LVO), mechanical thrombectomy (MT) is the gold-standard treatment. Mobile Stroke Units (MSUs) provide on-site diagnostic capabilities via computed tomography (CT) and have been shown to improve functional outcomes in stroke patients, but are cost-efficient only in urban areas. Blood biomarkers have recently emerged as possible alternative to cerebral imaging for LVO diagnosis. Prehospital LVO diagnosis offers the potential to transport patients directly to centers that have MT treatment available. In this study, we assess the accuracy of combining two biomarkers, HFABP and NT-proBNP, with clinical indicators to detect LVO using ultra-early prehospital blood samples. The study was registered in the German Clinical Trials Register (DRKS-ID: DRKS00030399).

Methods and analysis

We plan a multicenter prospective observational study with 800 patients with suspected stroke enrolled within 24 h of symptom onset. Study participants will be recruited at three sites (MSUs) in Berlin, Germany. Blood-samples will be taken pre-hospitally at the scene and tested for HFABP and NT-proBNP levels. Additional clinical data and information on final diagnosis will be collected and documented in an electronic case report form (eCRF). Sensitivity and specificity of the combination will be calculated through iterative permutation-response calculations.

Discussion

This study aims to evaluate the diagnostic capabilities of a combination of the biomarkers HFABP and NT-proBNP in LVO prediction. In contrast to most other biomarker studies to date, by employing MSUs as study centers, ultra-early levels of biomarkers can be analyzed. Point-of-care LVO detection in suspected stroke could lead to faster treatment in both urban and rural settings and thus improve functional outcomes on a broader scale.

Clinical trial registration

Deutsches Register klinischer Studien https://drks.de/search/de/trial/DRKS00030399, DRKS00030399
Research hotspots and frontiers in post-stroke dysphagia: a bibliometric analysis study
Authors: Guo Bilian , Liu Mengwei , Wang Zhiyong , Yan Zhipeng
Published: February 2024

Background

Dysphagia is a common complication of stroke that can result in serious consequences. In recent years, more and more papers on post-stroke dysphagia have been published in various journals. However, there is still a lack of bibliometric analysis of post-stroke dysphagia. This study visually analyzes the global research situation of post-stroke dysphagia from 2013 to 2022, aiming to explore the current research status, frontier trends, and research hotspots in this field.

Methods

Articles and reviews relevant to post-stroke dysphagia were obtained and retrieved from the Web of Science core collection database in the last 10 years (from 2013 to 2022). CiteSpace and Microsoft Excel 2019 were used for bibliographic analysis.

Results

A total of 1,447 articles were included in the analysis. The number of publications showed an overall upward trend, from 72 in 2013 to 262 in 2022. The most influential authors, institutions, journals, and countries were Hamdy S, University of London, Dysphagia, and the People's Republic of China. An analysis of keywords and the literature indicated that current studies in the field of post-stroke dysphagia focused on dysphagia and aspiration, dysphagia classification, dysphagia rehabilitation, and daily living.

Conclusion

This bibliometric analysis reveals the latest advancements and emerging trends in the field of post-stroke dysphagia, spanning the years 2013 to 2022. It highlights the paramount importance of conducting large-scale randomized controlled trials examining the efficacy of dysphagia screening protocols and non-invasive intervention techniques in improving the quality of life for these patients. Such research efforts hold significant academic implications for the development of evidence-based treatment strategies in this field.
Discovering optimal features for neuron-type identification from extracellular recordings
Authors: Haynes Vergil R. , Zhou Yi , Crook Sharon M.
Published: February 2024

Advancements in multichannel recordings of single-unit activity (SUA) in vivo present an opportunity to discover novel features of spatially-varying extracellularly-recorded action potentials (EAPs) that are useful for identifying neuron-types. Traditional approaches to classifying neuron-types often rely on computing EAP waveform features based on conventions of single-channel recordings and thus inherit their limitations. However, spatiotemporal EAP waveforms are the product of signals from underlying current sources being mixed within the extracellular space. We introduce a machine learning approach to demix the underlying sources of spatiotemporal EAP waveforms. Using biophysically realistic computational models, we simulate EAP waveforms and characterize them by the relative prevalence of these sources, which we use as features for identifying the neuron-types corresponding to recorded single units. These EAP sources have distinct spatial and multi-resolution temporal patterns that are robust to various sampling biases. EAP sources also are shared across many neuron-types, are predictive of gross morphological features, and expose underlying morphological domains. We then organize known neuron-types into a hierarchy of latent morpho-electrophysiological types based on differences in the source prevalences, which provides a multi-level classification scheme. We validate the robustness, accuracy, and interpretations of our demixing approach by analyzing simulated EAPs from morphologically detailed models with classification and clustering methods. This simulation-based approach provides a machine learning strategy for neuron-type identification.
Maize hydroxycinnamic acids: unveiling their role in stress resilience and human health
Authors: González-Rodríguez Tzitziki , García-Lara Silverio
Published: February 2024

Maize production is pivotal in ensuring food security, particularly in developing countries. However, the crop encounters multiple challenges stemming from climatic changes that adversely affect its yield, including biotic and abiotic stresses during production and storage. A promising strategy for enhancing maize resilience to these challenges involves modulating its hydroxycinnamic acid amides (HCAAs) content. HCAAs are secondary metabolites present in plants that are essential in developmental processes, substantially contributing to defense mechanisms against environmental stressors, pests, and pathogens, and exhibiting beneficial effects on human health. This mini-review aims to provide a comprehensive overview of HCAAs in maize, including their biosynthesis, functions, distribution, and health potential applications.
High-grade serous carcinoma of unknown primary origin associated with STIC clinically presented as isolated inguinal lymphadenopathy: a case report
Authors: Giancontieri Paola , Turetta Camilla , Barchiesi Giacomo , Pernazza Angelina , Pignataro Gemma , D’Onghia Giuliano , Santini Daniele , Tomao Federica
Published: February 2024

Serous tubal intraepithelial carcinoma (STIC) is a precancerous lesion of high-grade serous ovarian carcinoma (HGSOC). Usually, it arises from the fimbrial end of the tube, and it is associated with metastatic potential. On average, the time to progress from STIC to HGSOC is 6.5 years. Therefore, whenever a STIC lesion is found, surgical staging and prophylactic salpingectomy are recommended in order to prevent ovarian cancer. We report a rare case of a 45-year-old female patient who clinically presented an isolated right inguinal lymphadenopathy. The remaining clinical examination was normal. Therefore, an excisional biopsy of the lymph node was performed. Pathological analysis revealed a high-grade serous carcinoma, most likely of gynecological origin. Due to histological evidence, a computed tomography (CT) scan was carried out. There was no CT evidence of ovarian disease, pelvic involvement, intra-abdominal lymphadenopathies, metastatic disease, or ascites. All tumor markers were negative. The patient underwent laparoscopic hysterectomy and bilateral salpingo-oophorectomy followed by surgical staging. Surprisingly, pathological examination showed a STIC lesion in the fimbria of the left fallopian tube. We aim to report the potential capability of STIC to spread particularly through lymphatic pathways rather than peritoneal dissemination.
Clinical efficacy and safety of Chinese herbal injections in combination with platinum-based chemotherapy for advanced non-small cell lung cancer: a systematic review and meta-analysis of 140 randomized controlled trials
Authors: Cao Kangdi , Hu Shuaihang , Wang Dandan , Qiao Chenxi , Wang Zhuo , Wang Jinkun , Hou Wei
Published: February 2024

Background and aim

Chinese herbal injection (CHI) is a widely used preparation for advanced non-small cell lung cancer (NSCLC) treatment to alleviate the adverse drug reactions and enhance the clinical efficacy of chemotherapy. However, its efficacy and safety in combination with platinum-based chemotherapy (PBC) remain poorly understood owing to the lack of high-level evidence in the face of a wide variety of CHIs. Therefore, in this study, we aimed to explore the efficacy and safety of CHIs in combination with PBC regimens in the treatment of mid- and advanced NSCLC.

Methods

Systematic evaluation and meta-analysis were conducted as per the Preferred Reporting Project for Systematic Evaluation and Meta-Analysis Protocols (PRISMA-P). Seven databases were comprehensively searched for relevant randomized controlled trials (RCTs) through August 1, 2022. The quality of each study was evaluated based on the Cochrane Handbook for Systematic Reviews of Interventions. Statistical analysis was performed using Revman 5.3, with dichotomies expressed as risk ratio (RR) and 95% confidence interval (CI). Objective response rate (ORR) and disease control rate (DCR) were selected as the primary outcomes, with quality of life (QoL) and toxic side effects as secondary outcomes.

Results

A total of 140 RCTs were included in this study. The results of the meta-analysis suggested that, compared with PBC alone, PBC combined with CHIs significantly improved the ORR (RR=1.35, 95% CI: 1.30–1.41, P<0.001), DCR (RR=1.15, 95% CI: 1.13–1.18, P<0.001) and QoL (RR=1.29, 95% CI: 1.24–1.33, P<0.001). Moreover, the combination treatment reduced chemotherapy-induced leukopenia (RR=0.69, 95% CI: 0.64–0.75, P<0.001), anemia (RR=0.70, 95% CI: 0.62–0.79, P<0.001), thrombocytopenia (RR=0.68, 95% CI: 0.62–0.75, P<0.001), nausea and vomiting (RR=0.69, 95% CI: 0.63–0.76, P<0.001), diarrhea (RR=0.59, 95% CI: 0.48–0.73, P<0.001), and constipation (RR=0.68, 95% CI: 0.54–0.86, P=0.001).

Conclusion

According to the available evidence, CHIs in combination with PBC can improve clinical efficacy and reduce the toxic side effects in the treatment of advanced NSCLC. However, considering the study’s limitations, more rigorous and high-quality studies are needed to further confirm the results.

Systematic review registration

https://inplasy.com/inplasy-2022-1-0104/, identifier INPLASY202210104.
Antibody-mediated targeting of Claudins in cancer
Authors: Vonniessen Benjamin , Tabariès Sébastien , Siegel Peter M.
Published: February 2024

Tight junctions (TJs) are large intercellular adhesion complexes that maintain cell polarity in normal epithelia and endothelia. Claudins are critical components of TJs, forming homo- and heteromeric interaction between adjacent cells, which have emerged as key functional modulators of carcinogenesis and metastasis. Numerous epithelial-derived cancers display altered claudin expression patterns, and these aberrantly expressed claudins have been shown to regulate cancer cell proliferation/growth, metabolism, metastasis and cell stemness. Certain claudins can now be used as biomarkers to predict patient prognosis in a variety of solid cancers. Our understanding of the distinct roles played by claudins during the cancer progression has progressed significantly over the last decade and claudins are now being investigated as possible diagnostic markers and therapeutic targets. In this review, we will summarize recent progress in the use of antibody-based or related strategies for targeting claudins in cancer treatment. We first describe pre-clinical studies that have facilitated the development of neutralizing antibodies and antibody-drug-conjugates targeting Claudins (Claudins-1, -3, -4, -6 and 18.2). Next, we summarize clinical trials assessing the efficacy of antibodies targeting Claudin-6 or Claudin-18.2. Finally, emerging strategies for targeting Claudins, including Chimeric Antigen Receptor (CAR)-T cell therapy and Bi-specific T cell engagers (BiTEs), are also discussed.
Study of PARP inhibitors for breast cancer based on enhanced multiple kernel function SVR with PSO
Authors: Xue Haohan , Zhang Ruixuan , Yan Xudong , Wang Ruihan , Zhang Peijian
Published: February 2024

PARP1 is one of six enzymes required for the highly error-prone DNA repair pathway microhomology-mediated end joining (MMEJ) and needs to be inhibited when over-expressed. In order to study the PARP1 inhibitory effect of fused tetracyclic or pentacyclic dihydrodiazepinoindolone derivatives (FTPDDs) by quantitative structure-activity relationship technique, six models were established by four kinds of methods, heuristic method, gene expression programming, random forester, and support vector regression with single, double, and triple kernel function respectively. The single, double, and triple kernel functions were RBF kernel function, the integration of RBF and polynomial kernel functions, and the integration of RBF, polynomial, and linear kernel functions respectively. The problem of multi-parameter optimization introduced in the support vector regression model was solved by the particle swarm optimization algorithm. Among the models, the model established by support vector regression with triple kernel function, in which the optimalR2and RMSE of training set and test set were 0.9353, 0.9348 and 0.0157, 0.0288, and R2cvof training set and test set were 0.9090 and 0.8971, shows the strongest prediction ability and robustness. The method of support vector regression with triple kernel function is a great promotion in the field of quantitative structure-activity relationship, which will contribute a lot to designing and screening new drug molecules. The information contained in the model can provide important factors that guide drug design. Based on these factors, six new FTPDDs have been designed. Using molecular docking experiments to determine the properties of new derivatives, the new drug was ultimately successfully designed.
Historical and contemporary management of infantile hemangiomas: a single-center experience
Authors: Zou Yun , Wu Zhiping , Jin Pingliang , Fu Ronghua , Cheng Jun , Bai Hanxiang , Huang Mengyu , Huang Xiangqun , Yuan Hua
Published: February 2024

Objective: This study explores the 22-year evolution of Infantile Hemangiomas (IHs) treatment strategies at a single-center hospital, aiming to establish an individualized IHs management protocol.

Methods: Retrospective review of IHs infants 2000–2022 at the Department of Plastic Surgery, Jiangxi Provincial Children’s Hospital.

Results: In our study of 27,513 IHs cases, 72.2% were female, with the median age at first hospital visit being 25 days. The majority of cases had localized and superficial lesions primarily on the head, face, and neck (67.5%). Ulceration rates fell from 21.1% to 12.6% with the introduction of propranolol. Management strategies have shifted over time, with the proportion of cases undergoing expectant management dropping from 32.9% to 12.4%. Since 2008, 26.1% of patients were treated with oral propranolol, largely replacing corticosteroids. Topical β-blockers have been used in 12.1% of cases, leading to a reduction in local injection therapy from 20.8% to 13.2%. Laser therapy, introduced in 2016, has been used in 13.8% of cases, while surgical excision has dropped from 25.0% to 8.5% due to alternative treatment options. Combination therapy was used in 8.8% of cases post-2015, indicating a rising trend. Drawing from the evolution of IHs management strategies, an individualized protocol for the management of IHs was successfully established.

Conclusion: Treatment for IHs has evolved over recent decades, with less invasive medical interventions increasingly replacing more invasive methods. Furthermore, a personalized treatment protocol established in this study could boost the cure rate of IHs while minimizing potential side effects and complications.
The role of metal ions in the occurrence, progression, drug resistance, and biological characteristics of gastric cancer
Authors: Xiao Pengtuo , Li Changfeng , Liu Yuanda , Gao Yan , Liang Xiaojing , Liu Chang , Yang Wei
Published: February 2024

Metal ions exert pivotal functions within the human body, encompassing essential roles in upholding cell structure, gene expression regulation, and catalytic enzyme activity. Additionally, they significantly influence various pathways implicated in divergent mechanisms of cell death. Among the prevailing malignant tumors of the digestive tract worldwide, gastric cancer stands prominent, exhibiting persistent high mortality rates. A compelling body of evidence reveals conspicuous ion irregularities in tumor tissues, encompassing gastric cancer. Notably, metal ions have been observed to elicit distinct contributions to the progression, drug resistance, and biological attributes of gastric cancer. This review consolidates pertinent literature on the involvement of metal ions in the etiology and advancement of gastric cancer. Particular attention is directed towards metal ions, namely, Na, K, Mg, Ca, Fe, Cu, Zn, and Mn, elucidating their roles in the initiation and progression of gastric cancer, cellular demise processes, drug resistance phenomena, and therapeutic approaches.
Therapeutic potential of hydrogen sulfide in osteoarthritis development
Authors: Song Yunjia , Wu Siyu , Zhang Rong , Zhong Qing , Zhang Xuanming , Sun Xutao
Published: February 2024

The pathological mechanisms and treatments of osteoarthritis (OA) are critical topics in medical research. This paper reviews the regulatory mechanisms of hydrogen sulfide (H2S) in OA and the therapeutic potential of H2S donors. The review highlights the importance of changes in the endogenous H2S pathway in OA development and systematically elaborates on the role of H2S as a third gaseous transmitter that regulates inflammation, oxidative stress, and pain associated with OA. It also explains how H2S can lessen bone and joint inflammation by inhibiting leukocyte adhesion and migration, reducing pro-inflammatory mediators, and impeding the activation of key inflammatory pathways such as nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK). Additionally, H2S is shown to mitigate mitochondrial dysfunction and endoplasmic reticulum stress, and to modulate Nrf2, NF-κB, PI3K/Akt, and MAPK pathways, thereby decreasing oxidative stress-induced chondrocyte apoptosis. Moreover, H2S alleviates bone and joint pain through the activation of Kv7, K-ATP, and Nrf2/HO-1-NQO1 pathways. Recent developments have produced a variety of H2S donors, including sustained-release H2S donors, natural H2S donors, and synthetic H2S donors. Understanding the role of H2S in OA can lead to the discovery of new therapeutic targets, while innovative H2S donors offer promising new treatments for patients with OA.
Relationships of lower extremity and trunk asymmetries in elite soccer players
Authors: Maly Tomas , Hank Mikulas , Verbruggen Ferdia Fallon , Clarup Christian , Phillips Kirk , Zahalka Frantisek , Mala Lucia , Ford Kevin R.
Published: February 2024

In light of previous research highlighting the prevalence of asymmetries in soccer players and possible links to injury risks, there is a crucial gap in the biomechanical understanding of complex relationships between lower extremity and trunk asymmetries in elite soccer players. The purpose of this study was to investigate the level, relationships, and differences among twelve different parameters of strength, morphological, and neuromuscular asymmetries in elite soccer players.

Methods: Elite male soccer players (n = 25, age 21.7 ± 3.9 years) were tested in the following tests: bilateral fluid distribution, hip flexor range of motion, postural stability, isokinetic strength of knee extensors and flexors, isometric lateral trunk rotation strength, eccentric strength of knee flexors, isometric bilateral strength of hip adductors, and vertical ground reaction force in counter-movement jump-free arms, counter-movement jump, squat jump, and drop jump tests. One-way ANOVA, Pearson’s coefficient (r), and partial eta squared (ηp2) were used for data analysis.

Results: Significant differences in asymmetries were found in elite soccer players (F11,299 = 11.01, p < .01). The magnitude of asymmetry over 10% was in postural stability and drop jump parameters. The lowest magnitudes of asymmetries were in the fluid distribution of the lower limbs and the vertical ground reaction force during the take-off phase in squat jumps. The highest asymmetries between the dominant and non-dominant sides were found in postural stability and drop jump. A total of eleven significant correlations (p < 0.05, r = 0.41–0.63, R2 = 0.17–0.40) were detected between the analyzed asymmetries in elite soccer players. The lateral trunk rotation asymmetries were significantly correlated to vertical ground reaction force asymmetries and knee extensors.

Conclusion: Long-term exposure in elite soccer leads to unilateral biomechanical loading that induces abnormal strength and morphological adaptations in favor of the dominant side while linking lower limb and trunk strength asymmetries. By unraveling these complex relationships, we strive to contribute novel methods that could inform targeted training regimens and injury prevention strategies in the elite soccer community. The data should encourage future researchers and coaches to monitor and develop trunk strength linked to lower body kinematics.
Quantitative measurements of reactive oxygen species partitioning in electron transfer flavoenzyme magnetic field sensing
Authors: Austvold Chase K. , Keable Stephen M. , Procopio Maria , Usselman Robert J.
Published: February 2024

Biological magnetic field sensing that gives rise to physiological responses is of considerable importance in quantum biology. The radical pair mechanism (RPM) is a fundamental quantum process that can explain some of the observed biological magnetic effects. In magnetically sensitive radical pair (RP) reactions, coherent spin dynamics between singlet and triplet pairs are modulated by weak magnetic fields. The resulting singlet and triplet reaction products lead to distinct biological signaling channels and cellular outcomes. A prevalent RP in biology is between flavin semiquinone and superoxide (O2•−) in the biological activation of molecular oxygen. This RP can result in a partitioning of reactive oxygen species (ROS) products to form either O2•− or hydrogen peroxide (H2O2). Here, we examine magnetic sensing of recombinant human electron transfer flavoenzyme (ETF) reoxidation by selectively measuring O2•− and H2O2 product distributions. ROS partitioning was observed between two static magnetic fields at 20 nT and 50 μT, with a 13% decrease in H2O2 singlet products and a 10% increase in O2•− triplet products relative to 50 µT. RPM product yields were calculated for a realistic flavin/superoxide RP across the range of static magnetic fields, in agreement with experimental results. For a triplet born RP, the RPM also predicts about three times more O2•− than H2O2, with experimental results exhibiting about four time more O2•− produced by ETF. The method presented here illustrates the potential of a novel magnetic flavoprotein biological sensor that is directly linked to mitochondria bioenergetics and can be used as a target to study cell physiology.
Differential physiological and production responses of C3 and C4 crops to climate factor interactions
Authors: Opoku Emmanuel , Sahu Pranav Pankaj , Findurová Hana , Holub Petr , Urban Otmar , Klem Karel
Published: February 2024

This study examined the effect of the interactions of key factors associated with predicted climate change (increased temperature, and drought) and elevated CO2 concentration on C3 and C4 crop representatives, barley and sorghum. The effect of two levels of atmospheric CO2 concentration (400 and 800 ppm), three levels of temperature regime (21/7, 26/12 and 33/19°C) and two regimes of water availability (simulation of drought by gradual reduction of irrigation and well-watered control) in all combinations was investigated in a pot experiment within growth chambers for barley variety Bojos and sorghum variety Ruby. Due to differences in photosynthetic metabolism in C3 barley and C4 sorghum, leading to different responses to elevated CO2 concentration, we hypothesized mitigation of the negative drought impact in barley under elevated CO2 concentration and, conversely, improved performance of sorghum at high temperatures. The results demonstrate the decoupling of photosynthetic CO2 assimilation and production parameters in sorghum. High temperatures and elevated CO2 concentration resulted in a significant increase in sorghum above- and below-ground biomass under sufficient water availability despite the enhanced sensitivity of photosynthesis to high temperatures. However, the negative effect of drought is amplified by the effect of high temperature, similarly for biomass and photosynthetic rates. Sorghum also showed a mitigating effect of elevated CO2 concentration on the negative drought impact, particularly in reducing the decrease of relative water content in leaves. In barley, no significant factor interactions were observed, indicating the absence of mitigating the negative drought effects by elevated CO2 concentration. These complex interactions imply that, unlike barley, sorghum can be predicted to have a much higher variability in response to climate change. However, under conditions combining elevated CO2 concentration, high temperature, and sufficient water availability, the outperforming of C4 crops can be expected. On the contrary, the C3 crops can be expected to perform even better under drought conditions when accompanied by lower temperatures.
Friendship and self-harm: a retrospective qualitative study of young adults' experiences of supporting a friend who self-harmed during adolescence
Authors: Bilello Delfina , Townsend Ellen , Broome Matthew R. , Burnett Heyes Stephanie
Published: February 2024

Introduction

Self-harm amongst young people is becoming increasingly prevalent. Understanding, responding to, and supporting young people who self-harm is vital. Friends are typically the first and sometimes the only source of support sought by adolescents who self-harm. Despite their important role as confidants, friends' perspectives and experiences remain poorly understood.

Methods

We conducted retrospective qualitative semi-structured interviews, prompted by an adapted version of the Card Sort Task for Self-Harm (CaTS-FF), about the experiences of nine female young adults (18-20 years old) who supported a friend who self-harmed during adolescence. Data were analyzed using thematic analysis.

Results

Four themes were developed: (1) “I did not realize my friend was on the road to self-harm”: Friends' reactions to self-harm; (2) “That's what friends do”: the role of friends; (3) The impact of supporting a friend who self-harms; and (4) “They were quite formative years”: reflecting on growth through the experience.

Discussion

The present findings highlight the complex experiences of young people supporting a friend who self-harms. Despite being willing to take on the role of a supporter, participants experienced a range of difficult emotions and consequences. The temporal transition running through the four themes reflects the evolving nature of participants' attitudes, knowledge, and friendships. Overall, results highlight the unmet needs of adolescents supporting young people who self-harm, as well as identifying potential pathways to “support the supporters” toward resilience.
Organized crime requires dynamic decision making
Authors: Kerstholt José , Keijser Bas , Veldhuis Guido , Smits-Clijsen Eefje
Published: February 2024

It is extremely hard to successfully fight organized crime, not only because there are ambiguous and complex interactions between factors and actors, but also because organized crime is volatile and adaptive to changing conditions. This paper argues that, in addition to acquiring knowledge on criminal processes and behavior, we also need a better understanding of organizational decision-making processes to select the most effective and sustainable interventions aimed at organized crime. To date, one-shot decisions from a single organizational perspective are predominant in fighting organized crime. This type of decision often ignores the system response to the intervention. There is a need to acknowledge the dynamic nature of criminal behavior and networks, and to take that into account in the design of an intervention strategy. Such an approach entails a sequence of interdependent steps, iteratively applied, to reach sustainable effects. However, this way of decision making does not come naturally for most people. This paper concludes that investments should be made in training and decision support for teams fighting organized crime.
Determinants promoting and hindering physical activity in primary school children in Germany: a qualitative study with students, teachers and parents
Authors: Sell Louisa , Brandes Berit , Brandes Mirko , Zeeb Hajo , Busse Heide
Published: February 2024

Background

Determinants affecting children’s physical activity (PA) at an early age are of particular interest to develop and strengthen strategies for increasing the levels of children’s PA. A qualitative study was conducted to investigate the views of primary school-aged children, their teachers and parents regarding barriers and facilitators to engage in PA.

Methods

Focus groups were conducted separately with primary school children, parents and teachers in a city in Northern Germany between October 2021 and January 2022. The semi- structured focus groups with children and teachers took part in person within school, whereas the focus groups with parents took place online. Data were transcribed verbatim and analysed using thematic analysis. During analysis, the socio-ecological model was identified as useful to map the determinants mentioned and was consequently applied to organize the data.

Results

Teachers (n = 10), parents (n = 18) and children (n = 46) of five primary schools in Germany participated in the focus groups. Participants of the three groups identified similar barriers and facilitators of PA in primary school-aged children, ranging across all four layers of the socio-ecological model. The barriers encountered were the preferences of children for sedentary activities (individual characteristics), the preference of parents to control their child’s actions (microsystem), a lack of financial resources from parents and long sitting times in class (mesosystem), and barriers related to rainy weather and Covid-19 restrictions (exosystem). Facilitators mentioned were the childrens’ natural tendency to be active (individual characteristics), involvement and co-participation of parents or peers in engaging in PA, support provided by teachers and the school (microsystem), living in rural areas, having sufficient facilities and favorable weather conditions (exosystem).

Conclusion

A range of determinants promoting and hindering PA, ranging across all layers of the socio-ecological model were identified by children, parents and teachers in this study. These determinants need to be kept in mind when developing effective PA intervention programs for primary school-aged children. Future interventions should go beyond individual characteristics to also acknowledge the influence of childrens’ social surrounding, including parents, peers and teachers, and the wider (school) environment.
Serological screening in a large-scale municipal survey in Cascais, Portugal, during the first waves of the COVID-19 pandemic: lessons for future pandemic preparedness efforts
Authors: Seabra Sofia G. , Merca Francisco , Pereira Bernardo , Fonseca Ivo , Carvalho Ana Cláudia , Brito Vera , Alves Daniela , Libin Pieter , Martins M. Rosário O. , Miranda Mafalda N. S. , Pingarilho Marta , Pimentel Victor , Abecasis Ana B.
Published: February 2024

Background

Serological surveys for SARS-CoV-2 were used early in the COVID-19 pandemic to assess epidemiological scenarios. In the municipality of Cascais (Portugal), serological testing combined with a comprehensive socio-demographic, clinical and behavioral questionnaire was offered to residents between May 2020 and beginning of 2021. In this study, we analyze the factors associated with adherence to this municipal initiative, as well as the sociodemographic profile and chronic diseases clinical correlates associated to seropositivity. We aim to contribute with relevant information for future pandemic preparedness efforts.

Methods

This was a cross-sectional study with non-probabilistic sampling. Citizens residing in Cascais Municipality went voluntarily to blood collection centers to participate in the serological survey. The proportion of participants, stratified by socio-demographic variables, was compared to the census proportions to identify the groups with lower levels of adherence to the survey. Univariate and multivariate logistic regression were used to identify socio-demographic, clinical and behavioral factors associated with seropositivity.

Results

From May 2020 to February 2021, 19,608 participants (9.2% of the residents of Cascais) were included in the study. Based on the comparison to census data, groups with lower adherence to this survey were men, the youngest and the oldest age groups, individuals with lower levels of education and unemployed/inactive. Significant predictors of a reactive (positive) serological test were younger age, being employed or a student, and living in larger households. Individuals with chronic diseases generally showed lower seroprevalence.

Conclusion

The groups with low adherence to this voluntary study, as well as the socio-economic contexts identified as more at risk of viral transmission, may be targeted in future pandemic situations. We also found that the individuals with chronic diseases, perceiving higher risk of serious illness, adopted protective behaviors that limited infection rates, revealing that health education on preventive measures was effective for these patients.
Assessing heterogeneity of patient and health system delay among TB in a population with internal migrants in China
Authors: Sun Ruoyao , Wu Zheyuan , Zhang Hongyin , Huang Jinrong , Liu Yueting , Chen Meiru , Lv Yixiao , Zhao Fei , Zhang Yangyi , Li Minjuan , Yan Jiaqi , Jiang Hongbing , Zhan Yiqiang , Xu Jimin , Xu Yanzi , Yuan Jianhui , Zhao Yang , Shen Xin , Yang Chongguang
Published: February 2024

Backgrounds

The diagnostic delay of tuberculosis (TB) contributes to further transmission and impedes the implementation of the End TB Strategy. Therefore, we aimed to describe the characteristics of patient delay, health system delay, and total delay among TB patients in Shanghai, identify areas at high risk for delay, and explore the potential factors of long delay at individual and spatial levels.

Method

The study included TB patients among migrants and residents in Shanghai between January 2010 and December 2018. Patient and health system delays exceeding 14 days and total delays exceeding 28 days were defined as long delays. Time trends of long delays were evaluated by Joinpoint regression. Multivariable logistic regression analysis was employed to analyze influencing factors of long delays. Spatial analysis of delays was conducted using ArcGIS, and the hierarchical Bayesian spatial model was utilized to explore associated spatial factors.

Results

Overall, 61,050 TB patients were notified during the study period. Median patient, health system, and total delays were 12 days (IQR: 3–26), 9 days (IQR: 4–18), and 27 days (IQR: 15–43), respectively. Migrants, females, older adults, symptomatic visits to TB-designated facilities, and pathogen-positive were associated with longer patient delays, while pathogen-negative, active case findings and symptomatic visits to non-TB-designated facilities were associated with long health system delays (LHD). Spatial analysis revealed Chongming Island was a hotspot for patient delay, while western areas of Shanghai, with a high proportion of internal migrants and industrial parks, were at high risk for LHD. The application of rapid molecular diagnostic methods was associated with reduced health system delays.

Conclusion

Despite a relatively shorter diagnostic delay of TB than in the other regions in China, there was vital social-demographic and spatial heterogeneity in the occurrence of long delays in Shanghai. While the active case finding and rapid molecular diagnosis reduced the delay, novel targeted interventions are still required to address the challenges of TB diagnosis among both migrants and residents in this urban setting.
The use of objective structured clinical examination in dental education- a narrative review
Authors: Rayyan Mohammad Ramadan
Published: February 2024

The Objective Structured Clinical Examination (OSCE) is a performance-based assessment intended to assess medical students' clinical competency in a simulated, standardized environment. Because it measures the student's ability to use clinical knowledge, diagnostic skill, and decision-making, the OSCE is thought to be more objective than traditional tests. OSCE exams have been increasingly employed in dentistry schools, particularly in the last decade, and it is crucial to investigate instructors' and dental students’ experiences with this evaluation approach.
Strategies for evidence-based in head and neck cancer: practical examples in developing systematic review questions
Authors: Guerra Eliete Neves Silva , Amorim dos Santos Juliana , Coletta Ricardo D. , De Luca Canto Graziela
Published: February 2024

A systematic review (SR) requires several steps to be conducted. A major and initial challenge is to formulate a focused research question that may have high scientific relevance to provide evidence-based results and strategies. This narrative mini-review aims to present different categories of systematic reviews currently applied in Head and Neck Cancers (HNC), focusing on the strategies to provide results for evidence-based decision making. The SRs identified were of intervention, diagnostic testing, prognosis, in vitro and in vivo studies, prevalence, and epidemiological studies, and of association and risk factors. Focused questions that define the type of review, whether it is a therapy question (intervention), a question of prevalence or an outcome (prognosis) of disease, are discussed. Additionally, the importance in building interesting research questions and following all proposed steps to produce quality evidence are highlighted. This narrative mini-review may guide future research by showing how to perform and report relevant evidence in terms of HNC.
Using the pedicle screw-U rod system for the treatment of double-level lumbar spondylolysis and isthmic spondylolisthesis
Authors: Jiang Jinghao , Lin Tao , Chen Xia , Gao Rui , Zhou Xuhui
Published: February 2024

Objective

The aim of this study was to evaluate the efficacy of the pedicle screw-U rod system in treating double-level lumbar spondylolysis with or without spondylolisthesis.

Methods

A retrospective study was conducted. Twenty-six patients were included in this study and followed up at 3, 6, and 12 months. Patients without spondylolisthesis were treated with double U-shaped rods (group I), and patients with spondylolisthesis were treated with a lengthened U-shaped rod (group II). Japanese Orthopedic Association (JOA) scores, Oswestry disability index (ODI) scores, disc range of motion (ROM), intervertebral space height of fixed levels and adjacent levels, and grading the degeneration of adjacent segmental intervertebral discs were evaluated preoperatively and postoperatively.

Results

JOA and ODI scores improved significantly at 3 months both in groups I and II. The average bone grafting healing time was 6.1 ± 3.1 months for group I and 6 ± 2.8 months for group II. The intervertebral space heights of L4/L5 and L5/S1 were improved significantly at the final follow-up (p < 0.05 for both groups). Surgical segmental and adjacent segmental ROM had no significant change at the final follow-up, in comparison with data preoperatively (p > 0.05). No significant changes of intervertebral space height (L3/L4) and grading of intervertebral disc degeneration were noted before and after surgery (p = 0.141 and 0.484, respectively).

Conclusions

The pedicle screw-U rod system provided advantages of being easy in repairing symptomatic double-level lumbar spondylolysis. This technique improved disabilities of patients, preserved the lumbar spine ROM, and delayed the degeneration of adjacent segments.
Classification of feline hypertrophic cardiomyopathy-associated gene variants according to the American College of Medical Genetics and Genomics guidelines
Authors: Boeykens Fréderique , Abitbol Marie , Anderson Heidi , Dargar Tanushri , Ferrari Paolo , Fox Philip R. , Hayward Jessica J. , Häggström Jens , Davison Stephen , Kittleson Mark D. , van Steenbeek Frank , Ljungvall Ingrid , Lyons Leslie A. , Longeri Maria , Ohlsson Åsa , Peelman Luc , Dufaure de Citres Caroline , Smets Pascale , Turba Maria Elena , Broeckx Bart J. G.
Published: February 2024

Introduction

The correct labeling of a genetic variant as pathogenic is important as breeding decisions based on incorrect DNA tests can lead to the unwarranted exclusion of animals, potentially compromising the long-term health of a population. In human medicine, the American college of Medical Genetics (ACMG) guidelines provide a framework for variant classification. This study aims to apply these guidelines to six genetic variants associated with hypertrophic cardiomyopathy (HCM) in certain cat breeds and to propose a modified criterion for variant classification.

Methods

Genetic samples were sourced from five cat breeds: Maine Coon, Sphynx, Ragdoll, Devon Rex, and British Short- and Longhair. Allele frequencies were determined, and in the subset with phenotypes available, odds ratios to determine the association with HCM were calculated. In silico evaluation followed with joint evidence and data from other publications assisting in the classification of each variant.

Results

Two variants, MYBPC3:c.91G > C [A31P] and MYBPC3:c.2453C > T [R818W], were designated as pathogenic. One variant, MYH7:c.5647G > A [E1883K], was found likely pathogenic, while the remaining three were labeled as variants of unknown significance.

Discussion

Routine genetic testing is advised solely for the MYBPC3:c.91G > C [A31P] in the Maine Coon and MYBPC3:c.2453C > T [R818W] in the Ragdoll breed. The human ACMG guidelines serve as a suitable foundational tool to ascertain which variants to include; however, refining them for application in veterinary medicine might be beneficial.
Moniezia benedeni drives CD3+ T cells residence in the sheep intestinal mucosal effector sites
Authors: Chai Wenzhu , Yao Wanling , Pan Jing , Huang Zhen , Wang Baoshan , Xu Bin , Fan Xiping , He Wanhong , Wang Wenhui , Zhang Wangdong
Published: February 2024

Introduction

T cells are the core of the cellular immunity and play a key role in the regulation of intestinal immune homeostasis. In order to explore the impact Moniezia benedeni (M. benedeni) infection on distributions of CD3+ T cells in the small intestine of the sheep.

Methods

In this study, sheep pET-28a-CD3 recombinant plasmid were constructed and expressed in BL21 receptor cells, then the rabbit anti-sheep CD3 polyclonal antibody was prepared through recombinant protein inducing. The M. benedeni-infected sheep (infection group, n = 6) and healthy sheep (control group, n = 6) were selected, and the distributions of CD3+ T cells in intestinal laminae propria (LP) and mucous epitheliums were observed and analyzed systematically.

Results

The results showed that the rabbit anti-sheep CD3 polyclonal antibody had good potency and specificity. In the effector area of small intestine, a large number of CD3+ T cells were mainly diffusely distributed in the intestinal LP as well as in the mucous epitheliums, and the densities of intestinal LP from duodenum to jejunum to ileum were 6.01 cells/104 μm2, 7.01 cells/104 μm2 and 6.43 cells/104 μm2, respectively. Their distribution densities in mucous epitheliums were 6.71 cells/104 μm2, 7.93 cells/104 μm2 and 7.21 cells/104 μm2, respectively; in the infected group, the distributions of CD3+ T cells were similar to that of the control group, and the densities in each intestinal segment were all significantly increased (p < 0.05), meanwhile, the total densities of CD3+ T cells in duodenum, jejunum and ileum were increased by 33.43%, 14.50%, and 34.19%. In LP and mucous epitheliums, it was increased by 33.57% and 27.92% in duodenum; by 25.82% and 7.07% in jejunum, and by 27.07% and 19.23% in ileum, respectively.

Discussion

It was suggested that M. benedeni infection did not change the spatial distributions of CD3+ T cells in the small intestine of sheep, but significantly increased their densities, which lays a foundation for further research on the regulatory mechanism of sheep intestinal mucosal immune system against M. benedeni infection.