bluuebunny/crossref_metadata_2025 huggingface dataset:
Uncertainty Costs Optimization of Residential Solar Generators Considering Intraday Markets
Authors: Garcia-Guarin Julian , Alvarez David , Rivera Sergio
Published: November 2021

The uncertainty of solar generation and the bull market are unavoidable in energy dispatch. The purpose of this research is to validate an uncertainty cost function of residential photovoltaic energy in a real microgrid by varying the number of auctions in intraday markets. Therefore, the following procedure is proposed. First, the variability of photovoltaic generation is quantified through Monte Carlo simulations. Second, a statistical function calculates the variability costs of photovoltaic generation. Third, the uncertainty costs are estimated by varying intraday auction markets. Other complementary services are added to the network, such as battery exchange stations for electric vehicles, demand response loads, market power restrictions, and energy storage systems, which are estimated as total costs in an index ranking. The total costs are optimized in a benchmark microgrid and take complimentary services as a black box. Only the uncertainty costs of residential solar generators are discriminated. The main findings were that (1) the uncertainty costs have an error of less than 0.0168% compared to the Monte Carlo simulations and that (2) the uncertainty costs of solar generation are reduced with a decreasing trend to a more significant number of auction markets in intraday markets.
From Plantation to Cup: Changes in Bioactive Compounds during Coffee Processing
Authors: Bastian Februadi , Hutabarat Olly Sanny , Dirpan Andi , Nainu Firzan , Harapan Harapan , Emran Talha Bin , Simal-Gandara Jesus
Published: November 2021

Coffee is consumed not just for its flavor, but also for its health advantages. The quality of coffee beverages is affected by a number of elements and a series of processes, including: the environment, cultivation, post-harvest, fermentation, storage, roasting, and brewing to produce a cup of coffee. The chemical components of coffee beans alter throughout this procedure. The purpose of this article is to present information about changes in chemical components and bioactive compounds in coffee during preharvest and postharvest. The selection of the appropriate cherry maturity level is the first step in the coffee manufacturing process. The coffee cherry has specific flavor-precursor components and other chemical components that become raw materials in the fermentation process. During the fermentation process, there are not many changes in the phenolic or other bioactive components of coffee. Metabolites fermented by microbes diffuse into the seeds, which improves their quality. A germination process occurs during wet processing, which increases the quantity of amino acids, while the dry process induces an increase in non-protein amino acid γ-aminobutyric acid (GABA). In the roasting process, there is a change in the aroma precursors from the phenolic compounds, especially chlorogenic acid, amino acids, and sugars found in coffee beans, to produce a distinctive coffee taste.
Active and Robust Composite Films Based on Gelatin and Gallic Acid Integrated with Microfibrillated Cellulose
Authors: Luo Yinghua , Wu Yanbei , Wang Yali , Yu Liangli (Lucy)
Published: November 2021

Background: Gelatin is a renewable, biodegradable, and inexpensive food polymer. The insufficient mechanical and functional properties of gelatin-based films (GBF) restrict their commercial application in food packaging. This work proposed a facile strategy to prepare an active and robust GBF that has the potential to be used in food packaging. Methods: A strong and active GBF was prepared based on the principle of supramolecular chemistry via the incorporation of gallic acid (GA) as an active crosslinking agent and of microfibrillated cellulose (MFC) as a reinforcing agent. Results: Under the appropriate concentration (1.0 wt%), MFC was evenly dispersed in a gelatin matrix to endow the film with low surface roughness and compact structure. Compared with the GF, the tensile strength and elongation at break of the resultant film reached 6.09 MPa and 213.4%, respectively, representing the corresponding improvement of 12.8% and 27.6%. Besides, a significantly improved water vapor barrier (from 3.985 × 10−8 to 3.894 × 10−8 g·m−1·Pa−1·s−1) and antioxidant activity (from 54.6% to 86.4% for ABTS radical scavenging activity; from 6.0% to 89.1% for DPPH radical scavenging activity) of GBFs were also observed after introducing the aromatic structure of GA and nano-/microfibrils in MFC. Moreover, the UV blocking performance and thermal stability of GGF and GGCFs were also enhanced. Conclusions: this work paves a promising way toward facile preparation of multifunctional GBFs that have great potential to be used in fabricating active and safe food packaging materials for food preservation.
Two Rare Variants in PLAU and BACE1 Genes—Do They Contribute to Semantic Dementia Clinical Phenotype?
Authors: Gaweda-Walerych Katarzyna , Sitek Emilia J. , Borczyk Małgorzata , Berdyński Mariusz , Narożańska Ewa , Brockhuis Bogna , Korostyński Michał , Sławek Jarosław , Zekanowski Cezary
Published: November 2021

We have performed whole-genome sequencing to identify the genetic variants potentially contributing to the early-onset semantic dementia phenotype in a patient with family history of dementia and episodic memory deficit accompanied with profound semantic loss. Only very rare variants of unknown significance (VUS) have been identified: a nonsense variant c.366C>A/p.Cys122* in plasminogen activator, urokinase (PLAU) and a missense variant c.944C>T/p.Thr315Met in β-site APP-cleaving enzyme 1 (BACE1)—along with known disease-modifying variants of moderate penetrance. Patient-derived fibroblasts showed reduced PLAU and elevated BACE1 mRNA and protein levels compared to control fibroblasts. Successful rescue of PLAU mRNA levels by nonsense-mediated mRNA decay (NMD) inhibitor (puromycin) confirmed NMD as the underlying mechanism. This is the first report of the PLAU variant with the confirmed haploinsufficiency, associated with semantic dementia phenotype. Our results suggest that rare variants in the PLAU and BACE1 genes should be considered in future studies on early-onset dementias.
Between Consultation and Collaboration: Self-Reported Objectives for 25 Web-Based Geoparticipation Projects in Urban Planning
Authors: Babelon Ian , Pánek Jiří , Falco Enzo , Kleinhans Reinout , Charlton James
Published: November 2021

Web-based participatory mapping technologies are being increasingly harnessed by local governments to crowdsource local knowledge and engage the public in urban planning policies as a means of increasing the transparency and legitimacy of planning processes and decisions. We refer to these technologies as “geoparticipation”. Current innovations are outpacing research into the use of geoparticipation in participatory planning practices. To address this knowledge gap, this paper investigates the objectives of web-based geoparticipation and uses empirical evidence from online survey responses related to 25 urban planning projects in nine countries across three continents (Europe, North America, and Australia). The survey adopts the objectives of the Spectrum for Public Participation that range from information empowerment, with each category specifying promises about how public input is expected to influence decision-making (IAP2, 2018). Our findings show that geoparticipation can leverage a ‘middle-ground’ of citizen participation by facilitating involvement alongside consultation and/or collaboration. This paper constitutes a pilot study as a step toward more robust and replicable empirical studies for cross-country comparisons. Empowerment (or citizen control) is not yet a normative goal or outcome for web-based geoparticipation. Our evidence also suggests that information is pursued alongside other objectives for citizen participation, and therefore functions not as a “low-hanging fruit” as portrayed in the literature, but rather as a core component of higher intensities of participation.
Ferroptosis: A Double-Edged Sword in Gastrointestinal Disease
Authors: Xu Chengfei , Liu Ziling , Xiao Jiangwei
Published: November 2021

Ferroptosis is a novel form of regulated cell death (RCD) that is typically accompanied by iron accumulation and lipid peroxidation. In contrast to apoptosis, autophagy, and necroptosis, ferroptosis has unique biological processes and pathophysiological characteristics. Since it was first proposed in 2012, ferroptosis has attracted attention worldwide. Ferroptosis is involved in the progression of multiple diseases and could be a novel therapeutic target in the future. Recently, tremendous progress has been made regarding ferroptosis and gastrointestinal diseases, including intestinal ischemia/reperfusion (I/R) injury, inflammatory bowel disease (IBD), gastric cancer (GC), and colorectal cancer (CRC). In this review, we summarize the recent progress on ferroptosis and its interaction with gastrointestinal diseases. Understanding the role of ferroptosis in gastrointestinal disease pathogenesis could provide novel therapeutic targets for clinical treatment.
Risk Factors of Coexisting Septic Spondylitis and Arthritis: A Case-Control Study in a Tertiary Referral Hospital
Authors: Wang Sheng-Fen , Lai Po-Liang , Liu Hsiang-Fu , Tsai Tsung-Ting , Lin Yu-Chih , Li Yun-Da , Chiu Ping-Yeh , Hsieh Ming-Kai , Kao Fu-Cheng
Published: November 2021

Introduction: In patients under immunosuppression or severe sepsis, it is sometimes manifested as coexisting septic arthritis and spondylitis. The aim of this study is to evaluate and investigate the risk factors of infectious spondylitis associated with septic arthritis. Methods: The study retrospectively reviewed the patients diagnosed with infectious spondylitis between January 2010 and September 2018 for risk factors of coexisting major joint septic arthritis. Results: A total of 10 patients with infectious spondylitis and coexisting septic arthritis comprised the study group. Fifty matched patients with solely infectious spondylitis were selected as the control group. Major risk factors include preoperative C-reactive protein (p = 0.001), hypoalbuminemia (p = 0.011), history of total joint replacement (p < 0.001), duration of preoperative antibiotics treatment (p = 0.038) and psoas muscle abscess (p < 0.001). Conclusion: Infectious spondylitis and septic arthritis are thought of as medical emergencies due to their high mortality and morbidity. Our study evaluated 5 risk factors as significant major findings: hypoalbuminemia (<3.4 g/dL), higher preoperative CRP (>130 mg/L), psoas muscle abscess, longer preoperative antibiotics treatment (>8 days) and history of total joint replacement. Clinicians should pay attention to the patients with those five factors to detect the coexisting infections as early as possible.
AIDS Related Kaposi’s Sarcoma: A 20-Year Experience in a Clinic from the South-East of Romania
Authors: Arbune Manuela , Padurariu-Covit Monica-Daniela , Rebegea Laura-Florentina , Lupasteanu Gabriela , Arbune Anca-Adriana , Stefanescu Victorita , Tatu Alin-Laurentiu
Published: November 2021

Kaposi’s sarcoma (KS) was peculiarly described in the first notified cases of the acquired immunodeficiency syndrome as an opportunistic condition. However, the medical progress and the development of active antiretroviral therapy allowed the control of the HIV/AIDS epidemic, although the features of KS have changed throughout the past decades. The purpose of our study is to assess the epidemiological and clinical features of AIDS related KS in Romanian patients. A retrospective follow-up study was achieved in a single infectious diseases’ clinic from Galati—Romania, between 2001 and 2021. Referring to 290 new HIV diagnosed cases from our clinic retained in care, the prevalence of KS was 3.4%. The main characteristics of patients with KS are a median age of 33, a predominance of males, prevalent severe systemic forms of diseases, frequent association of past or concomitant tuberculosis, and context of immune reconstruction syndrome. The mortality rate was 70%. KS has occurred in patients with delayed HIV diagnoses and inadequate adherence to therapy. Early recognition of both infections, the close monitoring of latent or symptomatic tuberculosis, improving the antiretroviral adherence and raising the access to oncologic procedures in Romanian HIV patients could improve their prognosis related to KS.
A Clot Waveform Analysis Showing a Hypercoagulable State in Patients with Malignant Neoplasms
Authors: Kobayashi Mayu , Wada Hideo , Fukui Shunsuke , Mizutani Hiroki , Ichikawa Yuhuko , Shiraki Katsuya , Moritani Isao , Inoue Hidekazu , Shimaoka Motomu , Shimpo Hideto
Published: November 2021

(1) Objective: hypercoagulability in patients with malignant neoplasm were evaluated to examine the relationship with thrombosis. (2) Methods: clot waveform analysis (CWA)—activated partial thromboplastin time (APTT) and CWA—small amount of tissue factor induced FIX activation (sTF/FIXa) assays were performed in 92 patients with malignant neoplasm and the relationship between hypercoagulability and thrombosis was retrospectively examined. (3) Results: The study population included 92 patients with malignant neoplasms. Twenty-six (28.3%) had thrombotic diseases and 9 (9.8%) patients died within 28 days after the CWA. The peak time of the CWA-APTT could not show hypercoagulability in patients with malignant neoplasms. There were almost no significant differences in the peak times of the sTF/FIXa among patients with malignant neoplasms and healthy volunteers. In contrast, the peak heights of the CWA-sTF/FIXa in patients with various malignant neoplasms were significantly higher than those in healthy volunteers. Furthermore, among patients with malignant neoplasms, the peak heights of the sTF/FIXa in patients with thrombosis were significantly higher than those in patients without thrombosis. (4) Conclusions: although the routine APTT cannot evaluate the hypercoagulability, the peak heights of CWA-sTF/FIXa were significantly high in patients with malignant neoplasms, especially in those with thrombosis, suggesting that an elevated peak height of the CWA-sTF/FIXa may be a risk factor for thrombosis.
Thyroid Hormone Changes Related to Growth Hormone Therapy in Growth Hormone Deficient Patients
Authors: Kucharska Anna Małgorzata , Witkowska-Sędek Ewelina , Rumińska Małgorzata , Pyrżak Beata
Published: November 2021

The alterations in thyroid function during recombinant human growth hormone (rhGH) treatment have been reported by many authors since this therapy became widely available for patients with growth hormone deficiency (GHD). Decrease of thyroxine level is the most frequent observation in patients treated with rhGH. This paper presents literature data describing changes in thyroid function related to rhGH therapy and a current explanation of mechanisms involved in this phenomenon. The effect of GH on the hypothalamic-pituitary-thyroid (HPT) axis is dependent on a multilevel regulation beginning from influence on the central axis, thyroid, and extra-thyroidal deiodinases activity as well as the impact on thyroid hormone receptors on the end. Changes in central and peripheral regulation could overlap during rhGH therapy, resulting in central hypothyroidism or an isolated slight deficiency of thyroxine. The regular monitoring of thyroid function is recommended in patients treated with rhGH and the decision of levothyroxine (L-thyroxine) supplementation should be made in the clinical context, taking into account thyroid hormone levels, as well as the chance for satisfactory growth improvement.
Invasive and Non-Invasive Approaches of Electrical Stimulation to Improve Physical Functioning after Spinal Cord Injury
Authors: Dolbow David R. , Gorgey Ashraf S. , Sutor Tommy W. , Bochkezanian Vanesa , Musselman Kristin
Published: November 2021

This review of literature provides the latest evidence involving invasive and non-invasive uses of electrical stimulation therapies that assist in restoring functional abilities and the enhancement of quality of life in those with spinal cord injuries. The review includes neuromuscular electrical stimulation and functional electrical stimulation activities that promote improved body composition changes and increased muscular strength, which have been shown to improve abilities in activities of daily living. Recommendations for optimizing electrical stimulation parameters are also reported. Electrical stimulation is also used to enhance the skills of reaching, grasping, standing, and walking, among other activities of daily living. Additionally, we report on the use of invasive and non-invasive neuromodulation techniques targeting improved mobility, including standing, postural control, and assisted walking. We attempt to summarize the effects of epidural stimulation on cardiovascular performance and provide a mechanistic explanation to the current research findings. Future trends such as the combination of epidural stimulation and exoskeletal-assisted walking are also discussed.
Specific Autoantibodies in Neovascular Age-Related Macular Degeneration: Evaluation of Morphological and Functional Progression over Five Years
Authors: Prasuhn Michelle , Hillers Caroline , Rommel Felix , Riemekasten Gabriela , Heidecke Harald , Nassar Khaled , Ranjbar Mahdy , Grisanti Salvatore , Tura Aysegül
Published: November 2021

(1) Background: Altered levels of autoantibodies (aab) and their networks have been identified as biomarkers for various diseases. Neovascular age-related macular degeneration (nAMD) is a leading cause for central vision loss worldwide with highly variable inter- and intraindividual disease courses. Certain aab networks could help in daily routine to identify patients with a high disease activity who need to be visited and treated more regularly. (2) Methods: We analyzed levels of aab against Angiotensin II receptor type 1 (AT1-receptor), Protease-activated receptors (PAR1), vascular endothelial growth factor (VEGF) -A, VEGF-B, and VEGF-receptor 2 in sera of 164 nAMD patients. In a follow-up period of five years, we evaluated changes in functional and morphological characteristics. Using correlation analyses, multiple regression models, and receiver operator characteristics, we assessed whether the five aab have a clinical significance as biomarkers that correspond to the clinical properties. (3) Results: Neither the analyzed aab individually nor taken together as a network showed statistically significant results that would allow us to draw conclusions on the clinical five-year course in nAMD patients. (4) Conclusions: The five aab that we analyzed do not correspond to the clinical five-year course of nAMD patients. However, larger, prospective studies should reevaluate different and more aab to gain deeper insights.
Trop-2 Therapy in Metastatic Triple-Negative Breast Cancer in Italy: Clinical Opportunity and Regulatory Pitfalls
Authors: Bravaccini Sara , Maltoni Roberta
Published: November 2021

Trop-2 is an ideal candidate for targeted therapeutics because it is a transmembrane protein with an extracellular domain overexpressed in a wide variety of tumors, and is upregulated in normal cells. Consequently, several Trop-2-targeted drugs have recently been developed for clinical use, such as anti-Trop-2 antibodies. Sacituzumab govitecan, a Trop-2-directed antibody and topoisomerase inhibitor drug conjugate, was recently approved by the Food and Drug Administration (FDA) and European Medicines Agency (EMA) for the treatment of metastatic triple-negative breast cancer and metastatic urothelial cancer. In Italy, this treatment cannot be used in clinical practice because it has not yet been approved by the Agenzia Italiana del Farmaco (AIFA, Rome, Italy). In Italy, this is not a new problem, in fact, when a new compound is approved by the U.S. and Europe, there is often a delay in its approval for use. The adoption of universal guidelines and the standardization of Trop-2 evaluation is urgently needed.
Longitudinal Study of the Association between General Anesthesia and Increased Risk of Developing Dementia
Authors: Sohn Jong-Hee , Lee Jae Jun , Lee Sang-Hwa , Kim Chulho , Yu Hyunjae , Kwon Young-Suk , Kim Dong-Kyu
Published: November 2021

The association between exposure to general anesthesia (GA) and the risk of dementia is still undetermined. To investigate a possible link to the development of dementia in older people who have undergone GA, we analyzed nationwide representative cohort sample data from the Korean National Health Insurance Service. The study cohort comprised patients over 55 years of age who had undergone GA between January 2003 and December 2004 and consisted of 3100 patients who had undergone GA and 12,400 comparison subjects who had not received anesthesia. After the nine-year follow-up period, we found the overall incidence of dementia was higher in the patients who had undergone GA than in the comparison group (10.5 vs. 8.8 per 1000 person-years), with the risk being greater for women (adjusted HR of 1.44; 95% CI, 1.19–1.75) and those with comorbidities (adjusted HR of 1.39; 95% CI, 1.18–1.64). Patients who underwent GA showed higher risks for Alzheimer’s disease and vascular dementia (adjusted HR of 1.52; 95% CI, 1.27–1.82 and 1.64; 95% CI, 1.15–2.33, respectively). This longitudinal study using a sample cohort based on a nationwide population sample demonstrated a significant positive association between GA and dementia, including Alzheimer’s disease and vascular dementia.
Assessment of Land Cover Dynamics and Drivers of Urban Expansion Using Geospatial and Logistic Regression Approach in Wa Municipality, Ghana
Authors: Asempah Mawuli , Sahwan Wahib , Schütt Brigitta
Published: November 2021

The current trends of land use dynamics have revealed a significant transformation of settlement spaces. In the Wa Municipality of Ghana, the changes in land use and land cover are inspired by a plethora of driving forces. In this study, we assessed the geo-physical drivers of settlement expansion under land use dynamics in the Wa Municipality of Ghana. The study employed geospatial and remote sensing tools to map and analyse the spatio-temporal dynamics of the landscape, using Landsat satellite imageries: thematic mapper (TM), enhanced thematic mapper (ETM) and operational land imager (OLI) from 1990 to 2020. The study employed a binomial logistic regression model to statistically assess the geo-physical drivers of settlement expansion. Random forest (RF)–supervised classification based on spatio-temporal analyses generated relatively higher classification accuracies, with overall accuracy ranging from 89.33% to 93.3%. Urban expansion for the last three decades was prominent, as the period from 1990 to 2001 gained 11.44 km2 landmass of settlement, while there was 11.30 km2 gained from 2001 to 2010, and 29.44 km2 gained from 2010 to 2020. Out of the independent variables assessed, the distance to existing settlements, distance to river, and distance to primary, tertiary and unclassified roads were responsible for urban expansion.
Strengthening the Scientific Basis of Ecosystem Collapse Risk Assessments
Authors: Newton Adrian C.
Published: November 2021

Progress is being made in assessing the conservation status of ecosystems, notably through initiatives such as the IUCN Red List of Ecosystems (RLE) and the NatureServe Conservation Status Assessment (NCS). Both of these approaches consider conservation status in terms of the risk of ecosystem collapse. However, the scientific understanding of ecosystem collapse is still at a relatively early stage. Consequently, concerns have been raised regarding the scientific basis of ecosystem conservation assessments focusing on collapse risk. Here I explore how these concerns might potentially be addressed by considering how the concept is defined, and by briefly reviewing the theoretical basis of ecosystem collapse. I then examine the implications of recent research results for the design of ecosystem collapse risk assessments, and the challenges identified in those assessments conducted to date. Recommendations are made regarding how collapse risk assessments might be strengthened based on current scientific understanding, and how this understanding could be improved by further research. In addition, I examine the potential implications for conservation policy and practice if the scientific basis of collapse risk assessments is not strengthened in this way.
The Natural and Socioeconomic Influences on Land-Use Intensity: Evidence from China
Authors: Chen Longgao , Yang Xiaoyan , Li Long , Chen Longqian , Zhang Yu
Published: November 2021

Intensive land use can support sustainable socioeconomic development, especially in the context of limited land resources and high population. It is measured by land-use intensity that reflects the degree of land-use efficiency. In order to support decision-making for efficient land use, we investigated the mechanism whereby natural and socioeconomic factors influence land-use intensity from the perspectives of overall, region-, and city-based analysis, respectively. This investigation was conducted in Chinese cities using the multiple linear stepwise regression method and geographic information system techniques. The results indicate that: (1) socioeconomic factors have more positive impact on land-use intensity than natural factors as nine of the top 10 indicators with the highest SRC values are in the socioeconomic category according to the overall assessment; (2) education input variously contributes to land-use intensity because of the mobility of a well-educated workforce between different cities; (3) the increase in transportation land may not promote intensive land use in remarkably expanding cities due to the defective appraisal system for governmental achievements; and that (4) in developed cities, economic structure contributes more to land-use intensity than the total economic volume, whereas the opposite is the case in less-developed cities. This study can serve as a guide for the government to prepare strategies for efficient land use, hence promoting sustainable socioeconomic development.
Effect of Urban-Rural Income Gap on the Population Peri-Urbanization Rate in China
Authors: Han Bingyang , Ma Zhili , Liu Yong , Wang Mengmeng , Lin Yingchao
Published: November 2021

Attracted by the urban–rural income gap (URIG), a massive agricultural transfer population has flooded into cities and became a peri-urbanized population due to the restrictions of the household registration system. This trend eventually leads to the rising population peri-urbanization rate (PPUR), which is equal to the proportion of urban resident population with rural household registration in the total residents, and seriously affects the development of new-type people-oriented urbanization. The purpose of this study is to investigate the impact of URIG on PPUR at the national and regional levels using the spatial exploration analysis and spatial Durbin model from the perspective of comparative economic interests. Empirical results revealed that PPUR had high spatial agglomeration, as indicated by high values in Eastern China and low values in Central and Western China. Moreover, the PPUR of most provinces in China was rising, dominated by intermediate values. At the national level, URIG promoted the increase of PPUR in the province, but inhibited the increase of PPUR in nearby provinces. Except for household registration, other control variables, such as industrial structure, fixed asset formation rate, infrastructure, medical resources and land-centered urbanization, also contributed to the PPUR in the province. At the regional level, the URIG of Central and Western China contributed to the increase of PPUR, whereas in Eastern China it inhibited the increase of PPUR. The strong correlation of URIG and PPUR calls for relevant policies for narrowing URIG and reducing PPUR.
A Life Cycle Assessment of an Energy-Biochar Chain Involving a Gasification Plant in Italy
Authors: Marzeddu Simone , Cappelli Andrea , Ambrosio Andrea , Décima María Alejandra , Viotti Paolo , Boni Maria Rosaria
Published: November 2021

Life cycle assessment (LCA) is a fundamental tool for evaluating the environmental and energy load of a production cycle. Its application to renewable energy production systems offers the possibility of identifying the environmental benefits of such processes—especially those related to the by-products of production processes (i.e., digestion or biochar). Biochar has received worldwide interest because of its potential uses in bioenergy production, due to its coproducts (bio-oil and syngas), as well as in global warming mitigation, sustainable agriculture, pollutant removal, and other uses. Biochar production and use of soil is a strategy for carbon sequestration that could contribute to the reduction of emissions, providing simultaneous benefits to soil and opportunities for bioenergy generation. However, to confirm all of biochar’s benefits, it is necessary to characterize the environmental and energy loads of the production cycle. In this work, soil carbon sequestration, nitrous oxide emissions, use of fertilizers, and use of water for irrigation have been considered in the biochar’s LCA, where the latter is used as a soil conditioner. Primary data taken from experiments and prior studies, as well as open-source available databases, were combined to evaluate the environmental impacts of energy production from biomass, as well as the biochar life cycle, including pre- and post-conversion processes. From the found results, it can be deduced that the use of gasification production of energy and biochar is an attractive strategy for mitigating the environmental impacts analyzed here—especially climate change, with a net decrease of about −8.3 × 103 kg CO2 eq. Finally, this study highlighted strategic research developments that combine the specific characteristics of biochar and soil that need to be amended.
Prognostic Impact of Tumor-Associated Macrophages on Long-Term Oncologic Outcomes in Colorectal Cancer
Authors: Shin Hyeong Chan , Seo Incheol , Jeong Hasong , Byun Sang Jun , Kim Shin , Bae Sung Uk , Kwon Sun Young , Lee Hye Won
Published: November 2021

This study evaluated the correlation between tumor-associated macrophages (TAMs) and long-term oncologic outcomes in colorectal cancer (CRC). We evaluated TAMs based on the expression of CD68, CD11c, and CD163 as optimal markers via immunohistochemistry in 148 patients with CRC who underwent surgical resection between September 1999 and August 2004. A high proportion of CD68-positive macrophages were associated with the occurrence of distant metastasis. A low proportion of CD11c-positive macrophages were associated with unfavorable overall survival (OS) and disease-free survival. CD11c-positive macrophages were found to act as independent prognostic factors for OS. An analysis of our long-term data indicated that TAMs are significantly associated with OS and prognosis in CRC.
A Free-Energy Landscape Analysis of Calmodulin Obtained from an NMR Data-Utilized Multi-Scale Divide-and-Conquer Molecular Dynamics Simulation
Authors: Shimoyama Hiromitsu , Shigeta Yasuteru
Published: November 2021

Calmodulin (CaM) is a multifunctional calcium-binding protein, which regulates a variety of biochemical processes. CaM acts through its conformational changes and complex formation with its target enzymes. CaM consists of two globular domains (N-lobe and C-lobe) linked by an extended linker region. Upon calcium binding, the N-lobe and C-lobe undergo local conformational changes, followed by a major conformational change of the entire CaM to wrap the target enzyme. However, the regulation mechanisms, such as allosteric interactions, which regulate the large structural changes, are still unclear. In order to investigate the series of structural changes, the free-energy landscape of CaM was obtained by multi-scale divide-and-conquer molecular dynamics (MSDC-MD). The resultant free-energy landscape (FEL) shows that the Ca2+ bound CaM (holo-CaM) would take an experimentally famous elongated structure, which can be formed in the early stage of structural change, by breaking the inter-domain interactions. The FEL also shows that important interactions complete the structural change from the elongated structure to the ring-like structure. In addition, the FEL might give a guiding principle to predict mutational sites in CaM. In this study, it was demonstrated that the movement process of macroscopic variables on the FEL may be diffusive to some extent, and then, the MSDC-MD is suitable to the parallel computation.
A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant Xerophyta schlechteri during Dehydration
Authors: Gabier Hawwa , Tabb David L. , Farrant Jill M. , Rafudeen Mohamed Suhail
Published: November 2021

Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant Xerophyta schlechteri in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80–70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.
Cardiovascular Surgical Emergencies in France, before, during and after the First Lockdown for COVID-19 in 2020: A Comparative Nationwide Retrospective Cohort Study
Authors: Baudry Anna , Mariet Anne-Sophie , Benzenine Eric , Crespy Valentin , Bernard Chloé , Morgant Marie-Catherine , Bejot Yannick , Giroud Maurice , Bouchot Olivier , Steinmetz Eric , Quantin Catherine
Published: November 2021

Background: There are still gaps regarding the impact of the nationwide lockdown on non-COVID-19 emergency hospitalizations. This study aims to describe the trends in hospitalizations for cardiovascular surgical emergencies in France, before, during and after the first lockdown. Materials and Methods: All adults admitted for mechanical complications of myocardial infarction (MI), aortic dissection, aortic aneurysm rupture, acute and critical limb ischemia, circulatory assistance, heart transplantation and major amputation were included. This retrospective cohort study used the French National Hospital Discharge database. The numbers of hospitalizations per month in 2020 were compared to the previous three years. Results: From January to September 2020, 94,408 cases of the studied conditions were reported versus 103,126 in the same period in 2019 (−8.5%). There was a deep drop in most conditions during the lockdown, except for circulatory assistance, which increased. After the lockdown, mechanical complications of MI and aortic aneurysm rupture increased, and cardiac transplantations declined compared with previous years. Conclusion: We confirmed a deep drop in most cardiovascular surgical emergencies during the lockdown. The post-lockdown period was characterized by a small over-recovery for mechanical complications of MI and aortic aneurysm rupture, suggesting that many patients were able to access surgery after the lockdown.
Role of PTEN, PI3K, and mTOR in Triple-Negative Breast Cancer
Authors: Prvanović Mirjana , Nedeljković Milica , Tanić Nasta , Tomić Tijana , Terzić Tanja , Milovanović Zorka , Maksimović Zlatko , Tanić Nikola
Published: November 2021

Breast cancer is the most commonly occurring malignancy and the leading cause of cancer-related death in women. Triple-negative breast cancer (TNBC) is the most aggressive subtype and is associated with high recurrence rates, high incidence of distant metastases, and poor overall survival. The aim of this study was to investigate the PI3K/PTEN/Akt/mTOR pathway as one of the most frequently deregulated pathways in cancer. We aimed to explore the impact of PI3K and mTOR oncogenes as well as the PTEN tumor suppressor on TNBC clinical behavior, prognosis, and multidrug resistance (MDR), using immunohistochemistry and copy number analysis by quantitative real-time PCR. Our results revealed that loss of PTEN and high expression of PI3K and mTOR proteins are associated with poor outcome of TNBC patients. PTEN deletions appeared as a major cause of reduced or absent PTEN expression in TNBC. Importantly, homozygous deletions of PTEN (and not hemizygous deletions) are a potential molecular marker of metastasis formation and good predictors of TNBC outcome. In conclusion, we believe that concurrent examination of PTEN/PI3K/mTOR protein expression may be more useful in predicting TNBC clinical course than the analysis of single protein expression. Specifically, our results showed that PTEN-reduced/PI3K-high/mTOR-high expression constitutes a ‘high risk’ profile of TNBC.
Progesterone, Myo-Inositol, Dopamine and Prolactin Present in Follicular Fluid Have Differential Effects on Sperm Motility Subpopulations
Authors: Keyser Shannen , van der Horst Gerhard , Maree Liana
Published: November 2021

Considering the challenges surrounding causative factors in male infertility, rather than relying on standard semen analysis, the assessment of sperm subpopulations and functional characteristics essential for fertilization is paramount. Furthermore, the diagnostic value of sperm interactions with biological components in the female reproductive tract may improve our understanding of subfertility and provide applications in assisted reproductive techniques. We investigated the response of two sperm motility subpopulations (mimicking the functionality of potentially fertile and sub-fertile semen samples) to biological substances present in the female reproductive tract. Donor semen was separated via double density gradient centrifugation, isolated into high (HM) and low motile (LM) sperm subpopulations and incubated in human tubal fluid (HTF), capacitating HTF, HD-C medium, progesterone, myo-inositol, dopamine and prolactin. Treated subpopulations were evaluated for vitality, motility percentages and kinematic parameters, hyperactivation, positive reactive oxygen species (ROS), intact mitochondrial membrane potential (MMP) and acrosome reaction (AR). While all media had a significantly positive effect on the LM subpopulation, dopamine appeared to significantly improve both subpopulations’ functional characteristics. HD-C, progesterone and myo-inositol resulted in increased motility, kinematic and hyperactivation parameters, whereas prolactin and myo-inositol improved the LM subpopulations’ MMP intactness and reduced ROS. Furthermore, progesterone, myo-inositol and dopamine improved the HM subpopulations’ motility parameters and AR. Our results suggest that treatment of sub-fertile semen samples with biological substances present in follicular fluid might assist the development of new strategies for IVF treatment.
Acid Dentin Lysate Modulates Macrophage Polarization and Osteoclastogenesis In Vitro
Authors: Nasirzade Jila , Kargarpour Zahra , Panahipour Layla , Gruber Reinhard
Published: November 2021

Dentin prepared from extracted teeth is used as autograft for alveolar bone augmentation. Graft consolidation involves the acid lysis of dentin thereby generating a characteristic paracrine environment. Acid lysate of dentin is mimicking this environment. Acid dentin lysate (ADL) potentially targets hematopoietic cells thereby affecting their differentiation towards macrophages and osteoclasts; however, the question remains if ADL controls macrophage polarization and osteoclastogenesis. Here, we show that ADL reduced lipopolysaccharide (LPS)-induced macrophage polarization of the pro-inflammatory (M1) phenotype, indicated by attenuated Interleukin 1 (IL1), Interleukine 6 (IL6)and cyclooxygenase 2 (COX2) expression. This decrease in M1 macrophages was confirmed by the reduced phosphorylation and nuclear translocation of p65 in the LPS-exposed RAW 264.7 macrophages. Similarly, when RAW 264.7 macrophages were incubated with other agonists of Toll-like receptor (TLR) signaling e.g., FSL1, Polyinosinic-polycytidylic acid High Molecular Weight (Poly (1:C) HMW), Pam3CSK4, and imiquimod, ADL reduced the IL6 expression. We further show herein that ADL decreased osteoclastogenesis indicated by the reduced formation of multinucleated cell expressing cathepsin K and tartrate-resistant acid phosphatase in murine bone marrow cultures. Overall, our results suggest that acid dentin lysate can affect the differentiation of hematopoietic cells to M1 macrophage polarization and a decrease in osteoclastogenesis in bone marrow cultures.
Study of Grain Growth in a Ni-Based Superalloy by Experiments and Cellular Automaton Model
Authors: Liu Yan-Xing , Ke Zhi-Jiang , Li Run-Hua , Song Ju-Qing , Ruan Jing-Jing
Published: November 2021

The grain growth behavior in a typical Ni-based superalloy was investigated using isothermal heat treatment experiments over a holding temperature range of 1353–1473 K. The experimental results showed that the grain structure continuously coarsened as the holding time and holding temperature increased during heat treatment. A classical parabolic grain growth model was used to explore the mechanism of grain growth under experimental conditions. The grain growth exponent was found to be slightly above 2. This indicates that the current grain growth in the studied superalloy is mainly governed by grain boundary migration with a minor pinning effect from the precipitates. Then, the grain growth in the studied superalloy during isothermal heat treatment was modelled by a cellular automaton (CA) with deterministic state switch rules. The microscale kinetics of grain growth is described by the correlation between the moving velocity and curvature of the grain boundary. The local grain boundary curvature is well evaluated by a template disk method. The grain boundary mobility was found to increase with increasing temperature. The relationship between the grain boundary mobility and temperature has been established. The developed CA model is capable of capturing the dependence of the grain size on the holding time under different holding temperatures.
Recent Advances in the Surface Functionalization of Nanomaterials for Antimicrobial Applications
Authors: Khan Shahin Shah , Ullah Irfan , Ullah Sadeeq , An Ruipeng , Xu Haijun , Nie Kaili , Liu Chaoyong , Liu Luo
Published: November 2021

Innovations in nanotechnology have had an immense impact on medicine, such as in drug delivery, tissue engineering, and medical devices that combat different pathogens. The pathogens that may cause biofilm-associated nosocomial diseases are multidrug-resistant (MDR) bacteria, such as Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), including both Gram-positive and Gram-negative bacterial species. About 65–80% of infections are caused by biofilm-associated pathogens creating a move in the international community toward developing antimicrobial therapies to eliminate such pathogenic infections. Several nanomaterials (NMs) have been discovered and significantly employed in various antipathogenic therapies. These NMs have unique properties of singlet oxygen production, high absorption of near-infrared irradiation, and reasonable conversion of light to heat. In this review, functionalized NPs that combat different pathogenic infections are introduced. This review highlights NMs that combat infections caused by multidrug-resistant (MDR) and other pathogenic microorganisms. It also highlights the biomedical application of NPs with regard to antipathogenic activities.
Influence of Microfibrillated Cellulose Additive on Strength, Elastic Modulus, Heat Release, and Shrinkage of Mortar and Concrete
Authors: Barabanshchikov Yurii , Pham Hien , Usanova Kseniia
Published: November 2021

This work aimed to study the effect of a microfibrillated cellulose additive on strength, elastic modulus, heat release, and shrinkage of mortar and concrete. The dosage of the additive varies from 0.4 to 4.5% by weight of the cement. The change in strength with an increase in the dosage of the additive occurred in a wave-like manner. The uneven character of the change in the results also took place in the determination of heat release and shrinkage. In general, heat release and shrinkage decreased at increasing additive dosage. The additive showed the greatest decrease in the heat release of concrete at a content of 2%. The heat release of concrete practically differed little from the exotherm of the standard at an additive content of 1 and 1.5%. The addition of microfibrillated cellulose additive in small (0.5%) and large (1.5%) amounts reduced shrinkage compared to the reference, and at an intermediate content (1%), the shrinkage was higher than in the reference specimens. In this case, the water evaporation rate from concrete increased with an increase in the additive. With an increase in the additive dosage, the modulus of elasticity decreases. Thus, the microfibrillated cellulose additive provides concrete with lower values of the modulus of elasticity, heat release, and shrinkage, and the additive is recommended for use in concretes with increased crack resistance during the hardening period. The recommended additive content is 0.5% by weight of cement. At the specified dosage, it is possible to provide the class of concrete in terms of compressive strength C35/45.
Structural and Optical Modifications in the BaO-ZnO-LiF-B2O3-Yb2O3 Glass System after γ-Irradiation
Authors: Prabhu Nimitha S. , Somashekarappa Hiriyur Mallaiah , Sayyed M. I. , Osman Hamid , Alamri Sultan , Khandaker Mayeen Uddin , Kamath Sudha D.
Published: November 2021

A Yb3+-doped borate glass system was examined for the structural and optical modifications after γ-irradiation. Among the studied 10BaO-20ZnO-20LiF-(50-x)B2O3-xYb2O3 (x = 0.1, 0.5, 0.7, and 1.0 mol%) glasses, the 10BaO-20ZnO-20LiF-49.9B2O3-0.1Yb2O3 glass showed the highest thermoluminescence intensity, trap density, and trap depth. The glass was irradiated with the optimum γ-dose of 1 kGy towards the analysis of radiation-induced defects. The amorphous nature was preserved before and after irradiation. The glass density slightly increased after irradiation. The structural rearrangement was evident from the Fourier transform infrared spectroscopy by the appearance and disappearance of some bonds after γ-irradiation. The transformation of [BO4] units into [BO3] units and non-bridging oxygens was deduced. The color of the glass darkened after irradiation and the optical absorption intensity enhanced between 250 and 700 nm. The optical bandgap reduced and Urbach energy increased upon γ-dose exposure. The electron spin resonance of the irradiated glass exhibited two signals at g = 2.0167 and g = 1.9938, corresponding to the non-bridging oxygen hole center and Boron E’-center, respectively.