bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluación del efecto del tiempo de refrigeración sobre la integridad funcional de la membrana espermática en semen de padrillos de la línea Zangersheide
Authors:   , Espinoza Giron Adriana Antonell
Published: June 2022

La inseminación artificial en mejora genética equina es una técnica recurrente debido a la habitual lejanía geográfica entre los parentales seleccionados. La disponibilidad de semen fresco es fundamental para este fin y por ello se vuelve importante conocer el impacto del tiempo de refrigeración sobre la calidad seminal, ya que tiempos prolongados de refrigeración pueden deteriorar la integridad funcional de la membrana espermática y por tanto disminuir la tasa de éxito de preñez. Los tiempos y efectos se desconocen en la línea Zangersheide. Por consiguiente, el presente estudio tuvo como objetivo realizar una evaluación del efecto de la refrigeración a diferentes tiempos (0, 24 y 48 horas) sobre la integridad funcional de la membrana espermática en semen de padrillos de la línea Zangersheide; a través de la utilización del test hipoosmótico (HOST). Tres padrillos de fertilidad comprobada pertenecientes al Hospital Veterinario Central del Ejército Peruano fueron utilizados para colectar un total de quince muestras de semen (cinco muestras por padrillo). Las variables analizadas fueron tiempo de refrigeración (variable independiente) y porcentaje de espermatozoides con integridad funcional de la membrana espermática (variable dependiente). Los resultados de la presente investigación evidenciaron mediante la prueba de Kolmogorov-Smirnov (K-S) que en los tres tiempos evaluados de cada semental la integridad funcional de membrana sigue una distribución normal (P > 0.05). El porcentaje de integridad funcional de membrana espermática en los tres tiempos fue 70 ± 5.64 %, 55.2 ± 5.55 % y 38.2 ± 6.56 %, correspondientes a los tiempos de refrigeración 0, 24 y 48 horas respectivamente, existiendo diferencias (p<0.001) entre los 3 grupos (Anova y Post Test Tukey)...
Gestión del conocimiento y competencias digitales docentes del Colegio Alfonso Ugarte y docentes de los CEBA- Lima 2020
Authors:   , Pasapera Aguilar Marilu
Published: June 2022

La investigación propuesta, tuvo como objetivo, determinar la relación que existe entre la gestión del conocimiento y las Competencias Digitales Docentes del colegio Alfonso Ugarte y Docentes de los CEBA- Lima 2020. Fue una investigación de tipo aplicada de diseño descriptivo correlacional, no experimental y transversal. Su población estuvo conformada por 80 docentes. Se utilizó la técnica de recolección de datos a través de la encuesta y como instrumento, fue el cuestionario. El Alfa de Cronbach para la variable G.C. fue de 0.986 y para la variable competencia digital fue 0.987, la validez del instrumento se dio mediante el juicio de expertos. Los resultados estadísticos de correlación con el estadígrafo Rho de Spearman fueron, para la hipótesis general un r = 0.525 y un Sig. (Bilateral)= 0,000<0.05, por lo que se rechaza la hipótesis nula y aceptando la relación entre las variables. Por lo tanto, se llegó a la conclusión que sí existe una relación significativa entre la gestión del conocimiento y las competencias digitales docentes del colegio Alfonso Ugarte y docentes de los CEBA - Lima 2020.
El grado de desarrollo de la lectura crítica en los estudiantes de la carrera de Artes Escénicas de la Facultad de Ciencias Humanas de la Universidad Científica del Sur
Authors:   , Alva Torres Alejandro Martin
Published: June 2022

La presente investigación consiste en una evaluación diagnóstica del nivel de desarrollo de la competencia lectora, con primera y principal atención en la lectura crítica, que poseen los alumnos de la Carrera de Artes Escénicas de la Facultad de Ciencias Humanas, sobre todo en los cursos de Teoría y de Historia del Teatro. En tal sentido, esta investigación pretende generar oportunas reflexiones, y a partir de ellas formular recomendaciones sobre cómo regular o mejorar el desarrollo de la lectura crítica en los estudiantes de la Universidad Científica del Sur.
Seed Propagation Methods for Ratibida columnifera (Nutt.) Wooton & Standl.
Authors: Hopkins Kaitlin A. , Arnold Michael A. , Hall Charles R. , Pemberton H. Brent , Palma Marco A.
Published: June 2022

Variation in floral characteristics and growth habits within the native range of the North American wildflower Ratibida columnifera (Nutt.) Wooton & Standl. suggests potential for breeding and selection efforts to develop improved cultivars for commercial and residential landscapes. Experiments in seed propagation were performed to enable perpetuation of unique germplasms. Overnight hydration, storage condition variations, stratification and scarification, and seed maturation effects were assessed to determine impacts on viability and percent germination. Overnight hydration had no impact on percent germination. Germplasm had a significant effect on germination for all remaining experiments. Seed maintained viability at the same rate through 18 months, when slight reductions were noted. Cold storage at 3 °C had no effect on viability or percent germination of dry seed compared with storage at 23 °C. All three germplasms exhibited increased percent germination with some stratification period, and declined significantly in percent germination with all acid scarification treatments. Experiments indicated that most germplasms benefit from between 30 to 60 days of cold, moist stratification. There was a significant interaction effect among germplasms, location on the inflorescences, and maturity stages for R. columnifera. Data suggest that seed should be harvested as close as possible to when natural dispersal would occur for optimum germination. The degree of improvement in viability and percent germination associated with harvesting at various developmental stages, seed pretreatments, and storage conditions suggests that to achieve germination success, pretreatments should be used for propagation of seed from mature inflorescences and that variation can be expected within different genotypes of this species.
Evaluating the Use of Biostimulants for Indoor Hydroponic Lettuce Production
Authors: Gómez Sofía , Gómez Celina
Published: June 2022

Biostimulant products have various reported benefits for plant production in the field or using hydroponic systems in protected structures. However, limited information is available describing their potential use for indoor farming applications. Considering that lettuce (Lactuca sativa) is one of the most popular crops produced in commercial indoor farms, the objective of this study was to compare growth and quality of lettuce grown indoors using nine biostimulant products derived from humic substances, amino acids, hydrolyzed proteins, or seaweed extracts. ‘Monte Carlo’, ‘Fairly’, and ‘Lalique’ lettuce were grown hydroponically for 30 to 33 days under a daily light integral, day/night temperature, relative humidity, and carbon dioxide concentration of ≈13 mol·m‒2·day‒1, 22/21 °C, 70%, and 800 µmol·mol‒1, respectively. There were no positive effects from using any of the biostimulant products evaluated in our study as growth (leaf area, leaf number, shoot diameter, and shoot and root dry weight), yield (shoot fresh weight), and quality (bolting, tipburn index, leaf color, and SPAD index) of treated plants were generally similar to those from the untreated control. Applications from one seaweed extract caused slight negative growth effects, possibly due to phytotoxicity. Cultivar differences showed that Fairly plants had the highest susceptibility to tipburn and bolting, and none of the biostimulant products countered these symptoms. Overall, the products evaluated provided marginal advantages for indoor hydroponic lettuce production.
Two-parton scattering in the high-energy limit: climbing two- and three-Reggeon ladders
Authors: Falcioni Giulio , Gardi Einan , Maher Niamh , Milloy Calum , Vernazza Leonardo
Published: June 2022

We review recent progress on the calculation of scattering amplitudes
in the high-energy limit. We start by illustrating the shockwave
formalism, which allows one to calculate amplitudes as iterated
solutions of rapidity evolution equations. We then focus on our recent
results regarding 2\to 22→2
parton scattering. We present the calculation of the imaginary part of
the amplitude, at next-to-leading logarithmic accuracy in the
high-energy logarithms, formally to all orders, and in practice to 13
loops. We then discuss the computation of the real part of the amplitude
at next-to-next-to-leading logarithmic accuracy and through four loops.
Both computations are carried in full colour, and provide new insight
into the analytic structure of scattering amplitudes and their infrared
singularity structure.
On the modeling of $t\bar{t}W^\pm$ signatures at the LHC
Authors: Kraus Manfred
Published: June 2022

We discuss our recent progress on improving the theoretical
description of the pp \to t\bar{t}W^\pmpp→tt‾W±
process with particular focus on fiducial signatures that include
top-quark decays. We employ a wide range of techniques from parton
shower matching, narrow width approximation to full off-shell
computations to assess the importance of the different modeling
approaches.
New developments in KKMChh: Quark-level exponentiated radiative corrections and semi-analytical results
Authors: Yost Scott , Dittrich M. , Jadach Stanislaw , Ward B.F.L. , Was Zbigniew Andrzej
Published: June 2022

We describe a new semi-analytical program, KKhhFoam, which provides a simplified framework for testing the amplitude-level exponentiation scheme (CEEX) of the full KKMChh program in the semi-soft limit. The structure of the KKhhFoam integrand is also helpful for elucidating the structure of CEEX. We also discuss the representation of ISR in KKMChh and compare the ISR added by KKMChh to the effect of switching to a QED-corrected PDF, at the individual quark level, and suggest a new approach to running KKMChh with QED-corrected PDFs.
TO WHAT EXTENT HAS BETI BACHAO BETI PADHAO BEEN SUCCESSFUL IN IMPACTING GIRL EDUCATION IN INDIA?
Authors: Garg Tanisha
Published: June 2022

Beti Bachao, Beti Padhao (save the girl child, educate the girl child)one of the first scheme introduced by the government India in 2015 aimed at generating awareness about gender empowerment and the importance of education for the girl child. This paper evaluates whether the policy has been successful in impacting girl education in India by considering factors such as change in enrollment ratio of girls in secondary education, change in child sex ratio, effectiveness of awareness drives and the allocation of funds. Ourresults indicate that this policy alone cannot be successful in changing the attitudes of people. To makeawareness drives more effective, my research sheds light on how the funds need to be diverted towards district level engagement rather than at the national level.
THE BARRIERS TO ACHIEVING EDUCATION FOR SUSTAINABLE DEVELOPMENT IN NAGALAND, INDIA
Authors:   Assistant, Professor Department of Education, University of Science and Technology Meghalaya., Rudupra Rokono
Published: June 2022

Nagalands geographical location, combined with the lack of human and physical infrastructure, has been a hurdle to the growth and development of education. The ecological make-up of the state as a resource-filled frontier, belonging to people who place great importance on their culture and tradition, has framed the backdrop against which they have experienced changes brought about not only by climate change but also by modernization. This poses an important question that this paper aims to answer, which is, what is the role of education in a society with varyingnotionsof sustainabilityshapedby differing experiences?
COMPARATIVE STUDY BETWEEN DICLOFENAC SUPPOSITORY AND COMBINED ORAL DICLOFENACWITH LOCAL ANAESTHETIC AGENT IN PAIN MANAGEMENT IN ACUTE FISSURE IN ANO IN OUT PATIENT DEPARTMENT CARE
Authors:   Junior Resident, Department of General Surgery MGM Medical College And Hospital Navi Mumbai., Doshi Samit , Reza Ali ,   Professor, Department of General Surgery MGM Medical College And Hospital Navi Mumbai., Kadam Sameer ,   Professor and Head, Department of General Surgery MGM Medical College And Hospital Navi Mumbai., Holkar Deepak ,   Assistant Professor, Department of General Surgery MGM Medical College And Hospital Navi Mumbai., Vardey Mohit ,   Intern, Department of General Surgery MGM Medical College And Hospital Navi Mumbai., Desai Megh ,   Intern, Department of General Surgery MGM Medical College And Hospital Navi Mumbai.
Published: June 2022

Introduction: Anal fissure is a common problem that causes significant morbidity in a young and otherwise healthy population. Acute fissure in ano is a very common problem faced by Indian population. Though it is a very common problem still the pain relief has always been an unsolved issue. There are various pain management modalities for fissure in ano. The objectives of this study is to evaluate and compare the efficacy of diclofenac suppository versus combined oral diclofenac with local anaesthetic agent in prevention of pain in acute fissure in ano and to achieve an effective, cheaper and an easy method to relieve pain in patients with acute fissure in ano in Out patient department care. Diclofenac suppositories are opted as they are easily available and patient could insert it without anyones assistance and hence not hospitalized for intravenous administration of analgesics. Methods: A study was conducted over 100 cases over a span of 1 year in a tertiary health care centre. Group A patients were treated with Diclofenac suppository 100 mg twice a day for 5 days with glycerine for lubrication at anal verge and Group B patients were treated with Oral Diclofenac 75 mg twice a day for 5 days plus Lignocaine Jelly for local anaesthetic. The pain score was noted in both the groups. All acute fissure in ano cases were included in the study. Results: The pain score of Group A cases was significantly lower than Group B cases. Conclusion: It was noted that the pain relief was significant in patients in Diclofenac suppository alone compared to combined Oral Diclofenac and Lignocaine jelly and thus we can achieve significant pain reduction on Out Patient Department basis with Diclofenac suppositories alone within seven days. Thus an effective, safe, cheap and an easy modality for pain relief in acute fissure in ano with good results could be achieved.
INFLUENCE OF POSTOPERATIVE ASTIGMATISM ON UNCORRECTED NEAR VISUAL ACUITY IN PSEUDOPHAKIA
Authors: A.R. Suresha , K. Shalaka , S. Hikkalagutti Kruthika
Published: June 2022

Aims: The impact of astigmatism on near visual acuity in pseudophakia patients who underwent manual small incision cataract surgery. Settings and Design: Randomized, cross-sectional, hospital-based observational study Methods and Material: 200 eyes of 200 pseudophakic patients with BCVA for distance 6/9 or better was considered. UCNV was recorded at 33 cms by giving only spherical correction used in distance correction without cylindrical correction. Residual astigmatism was noted , near visual acuity pertaining to astigmatism was compared and tabulated. PCIOL in the bag or sulcus was also noted with dilated pupil on slit lamp examination . Results:67% were ATR astigmatism 11% were WTR astigmatism, 11% had oblique astigmatism and 12% of them had no astigmatism. In ATR group, 30%, 26%, 15% of patients were able to read N12, N10, N8 vision respectively. In WTR group, 33%, 14%, 19% of patients read N8, N10,N12 respectively. 67% eyes belonged to cylindrical power range of -1.50 D to -0.50 D, 50% of them in this range read N8,N10. 138 patients had in the bag IOL, 62 of them had IOL in sulcus. Among those patients with in the bag IOL, 88 were in range of -0.50 to -1.50, out of which 19 had N8, 25 had N10, patients with IOL in sulcus, 31 were in range of -0.50 to -1.50, out of which 5 had N8, 10 had N10. Conclusions:Near acuity was relatively poor without astigmatism, and it improved with up to 0.5 to 1.5 D of simple myopic astigmatism. Near acuity deteriorated with hyperopic astigmatism for different meridians. Nearly 50% could read N8, N10 with only distance vision spherical correction.ATR astigmatism produced significantly better reading performance than WTR astigmatism. In the bag IOL had better near visual outcome as compared with in the sulcus IOL.
AMMONIUM NICKEL SULFATE (TUTTONS SALT) MEDIATED RAPID AND FACILE SYNTHESIS OF SCHIFF BASE UNDER ENVIRONMENTAL FRIENDLY CONDITION
Authors:   Department of Chemistry, Anwarul-Uloom College, New Malleypally, Hyderabad, India., Musthaq Sadiya , Begum Noorunnisa ,   Department of Chemistry, Anwarul-Uloom College, New Malleypally, Hyderabad, India., Sameena Aziz Syeda ,   Synergy Community Welfare Research Center (SCWRC), Head office, Panacea, Synergy India Foundation, 4th Floor, TSWREIS building, Masab Tank Hyderabad - 500028., Arifuddin Mohammed ,   Department of Chemistry, Anwarul-Uloom College, New Malleypally, Hyderabad, India.,   Synergy Community Welfare Research Center (SCWRC), Head office, Panacea, Synergy India Foundation, 4th Floor, TSWREIS building, Masab Tank Hyderabad - 500028.
Published: June 2022

In the present study a rapid and facile method for the synthesis of schiff bases (3a-z & 3aa-af) has been developed by using Ammonium Nickel Sulfate (ANS) (Tuttons Salt) as catalyst. Further more, the reaction is selective as only benzaldehydes are reacted where as acetophenone failed to perform this reaction. The present method is very rapid with simple work up and gives the products in good to excellent yield. The reaction is totally carried out under environmentally friendly condition by using green solvent MeoH and water in 1:1 ratio. Thus it is anticipated that the present newly developed method will give ample chemical space to the chemist to prepare the Schiff bases in rapid and envirnmentaly benign conditions.
IMPROVEMENT IN CHILDCARE HEALTH VIA MEDICAL EXPANSION?A RANDOMIZED CONTROL TRIAL
Authors:   Assistant Professor, Department of Community Medicine, Rajshree Medical Research Institute, Bareilly, Uttar Pradesh India. Pin code 243501., Ghos Ahmed , Hera Parwez ,   Assistant Divisional Medical Officer, Izzatnagar Division Railway Hospital, Indian Railways, Izatnagar, Bareilly,Uttar Pradesh India 243122.
Published: June 2022

Medical expansion has become a dynamic in India and that is required to control the increased health illness and other human problems. Medical expansion must be multidimensional and include two health care systems:- increasing medical investment and medical professionalization. We find medical investment and medial professionalization significantly improve a wide range of outcomes, including life expectancy and decreased mortality rate in children. We also found that medical expansion is negatively associated with childhood obesity and socio economic conditions are positively associated with the childcare health.
Octreotide in the treatment of acromegaly – the possibilities of high-dose therapy
Authors: Ilovayskaya I. A.
Published: June 2022

Octreotide is a first-generation somatostatin analog that has been used for 40 years for the medical treatment of acromegaly, both after neurosurgical intervention and as first-line treatment. The frequency of biochemical control against the background of extended-acting octreotide varies from 25 to 56% depending on growth hormone and IGF-1 levels at the disease debut, presence of previous surgery, patient gender and age, treatment compliance and the dose of octreotide used. Longterm clinical experience with prolonged-acting octreotide demonstrates that more than half of patients require an increase in dosage to 30 mg or higher. If during treatment with Octreotide in a dose of 30 mg for 3 months there is no normalization of IGF-1 level, but there was a decrease of 50% or more of the initial level, further dose increase to 40 mg is possible, because this increases the effectiveness of treatment without increasing the frequency of side effects. Foreign researchers have shown that high doses of Octreotide (60 mg every 28 days) can improve biochemical control in patients who have not fully responded to therapy with doses of 30–40 mg of extended-acting Octreotide. Further studies are needed to determine the optimal dose of prolonged octreotide in acromegaly therapy, both at the start of treatment and during treatment. Management of patients by a team of specialists involved in the treatment of pituitary tumors will allow faster achievement of biochemical control of acromegaly.
Inhibitors of dipeptidyl-peptidase-4: obvious and probable (literature review)
Authors: Ortenberg E. A. , Suplotova L. A.
Published: June 2022

The purpose of the presented literature review was an attempt to sum up current estimates of the effect of the use of dipeptidyl peptidase-4 inhibitors (iDPP-4) in the algorithms of both traditional (diabetes mellitus) and a number of alternative nosologies, in particular, oncological and neurological pathology, as well as a new coronavirus infection (COVID-19). To do this, the most large-scale (as a rule) publications of 2018–2021 devoted to the problems under consideration were analyzed. The search was carried out by keywords in the Pubmed information base (ncbi.nlm.nih.gov). Factors contributing to the widespread use of IDP-4 in clinical practice are both pharmacologically clear mechanism of action and efficacy, as well as the possibility of oral use, a successful pharmacokinetic profile, low toxicity, in particular, a low risk of hypoglycemia. Newly obtained data on the mechanisms of mechanisms are discussed. Renoprotective action, the presence of cardioprotection is debated. The biochemical prerequisites for the possible effectiveness of iDPP-4 as blockers of the development of a hyperimmune reaction that causes, in particular, the severe course of the new coronavirus infection are discussed. At the same time, the results of studies of various designs are categorically compared, indicating both in favor of the use of iDPP-4 in patients with COVID-19, and not noticing its expediency. It is concluded that, given the large-scale biochemical role of DPP-4, it is important both to continue the active use of its inhibitors in diabetes mellitus, and to expand attempts to use them in a number of other nosologies, including COVID-19.
Features of the course of type 2 diabetes mellitus in young people
Authors: Ovsyannikova A. K. , Zubareva A. Yu.
Published: June 2022

The increase in the prevalence of type 2 diabetes mellitus (T2DM) worldwide in young people determines the high relevance in studying the course of this disease. There are difficulties in awareness of this pathology in young people, both in specialists and in patients due to the fact that the long-term outcomes of T2DM in young people are poorly understood. This leads to late diagnosis of diabetes and longer exposure to hyperglycemia leads to high risks of microand macrovascular complications. Clinical symptoms of T2DM with a debut at a young age are different in patients, so this disease is not always diagnosed on time. T2DM in young people (18–45 years) has a more aggressive course, the decrease in the level of β-cells occurs faster than in patients with late-onset T2DM. The risk of developing complications in T2DM with onset at a young age is higher than in late-onset diabetes, mainly due to the longer duration of the disease. With a duration of T2DM in young people of 13.3 ± 1.8 years it was shown that the incidence of nephropathy, neuropathy and retinopathy was 54.8%, 32.4% and 13.7%, respectively. According to the literature, in patients with T2DM at a young age, life expectancy is reduced by 14 and 16 years in males and females, respectively. The course of T2DM is more aggressive in relation to young patients than to middle-aged and elderly patients. T2DM with a debut at a young age is a socially significant disease, due to a decrease in the quality of life, the development of diabetic complications and early disability of the working population.
Projection into the future: combination of orphenadrine and diclofenac in patients with bone metastases
Authors: Abuzarova G. R. , Sarmanaeva R. R. , Kuznetsov S. V. , Bychkova N. M. , Brazhnikova Yu. V.
Published: June 2022

Introduction. Radiation therapy is an effective method of treating pain in patients with bone metastases, but during radiotherapy there is often an increase in pain. The presence of a new drug containing a combination of orphenadrine and diclofenac in oncologists expands the possibilities of analgesia.

Aim. To evaluate the efficacy and safety of the use of a fixed combination of diclofenac and orphenadrine in patients with pain due to metastatic bone damage during radiotherapy.

Materials and methods. The patients were randomized into two groups: the first group (n = 30) received the drug Neodolpasse intravenously once a day for two days; the second - 2 times a day (n = 30) - 2 days. Efficacy was evaluated based on the following measurements: intensity of pain by NRS immediately after administration of the drug, after 30 minutes, after 1, 2, 4 and 24 hours, the dynamics of daily doses of analgesics.

Results and discussion. In group 1, a meaningful decrease in pain intensity (p < 0.05) from 48.7 ± 10.6 mm to 26.8 ± 10.7 mm was achieved 30 minutes after the completion of infusion. The pain intensity measured by the Numeric Rating Scale (NRS) reached its minimum value 2 hours after the completion of infusion (22.5 ± 12.0 mm). In group 2, the pain intensity decreased 30 minutes after the completion of infusion, a meaningful decrease (p < 0.05) in pain intensity was achieved from 56.5 ± 9.8 mm to 34.0 ± 10.5 mm, and up to 29.8 ± 10.2 mm after the second dose. The pain intensity decreased by 45.9% in group 1 and by 47.9% in group 2 towards the end of the Neodolpasse therapy cycle. During the study, two mild to moderate adverse events (nausea, drop in blood pressure) that did not require any significant drug therapy were observed.

Conclusion. The results of the study confirm that the use of the drug according to the proposed method is a safe and effective way of treating pain syndrome during radiotherapy in patients with metastatic bone damage.
Alpelisib therapy: from theory to practice
Authors: Lubennikova E. V. , Titova T. A. , Ganshina I. P.
Published: June 2022

Before the development and implementation of the first PI3K inhibitor (alpelisib), the presence of a mutation in the PIK3CA gene had only prognostic value: it determined the unfavorable course of luminal HER2-negative metastatic breast cancer (testing for mutations was not part of routine screening methods). Achievements in the treatment of HR+HER2- mBC are primarily associated with the use of CDK4/6 inhibitors, which allowed not only a significant increase in the median progression-free survival while maintaining high quality of life, but also significantly increased overall survival of patients with luminal HER2-negative metastatic breast cancer. However, subgroup analyses demonstrate that the presence of the PIK3CA mutation is an independent factor in decreasing progression-free time and overall survival, even in patients treated with CDK4/6 inhibitors. Mutations of the PIK3CA gene are diagnosed in 30-40% of luminal metastatic breast cancer patients, they are associated with an increased risk of relapse and disease progression, are associated with a significant reduction in survival rates and treatment effectiveness, and determine the development of primary and secondary resistance to endocrine therapy. Standard endocrine therapy with fulvestrant combined with alpelisib has significantly improved treatment outcomes in patients with HR+HER2-metastatic breast cancer with the PIK3CA mutation who previously received treatment for advanced disease or had progression during adjuvant therapy. This combination is now included in all major international guidelines and is a priority therapy option. Testing for PIK3CA mutations is the current diagnostic standard in luminal HER2-negative mBC. The review presents an update of the main clinical trials with alpelisib, treatment results from real clinical practice, and also considers aspects of use in pretreated patients with different medical history. The article outlines the main recommendations for the prevention and correction of adverse events, and presents our own experience of using alpelisib in a patient with a classic course of breast cancer with a PIK3CA mutation.
Olaparib maintenance therapy after frontline chemotherapy in patients with BRCA-mutated ovarian cancer: real-world results in expanded access program
Authors: Rumyantsev A. A. , Tyulyandina A. S. , Pokataev I. A. , Israelyan E. R. , Abramov M. Е. , Lud H. N. , Tyulyandin S. A.
Published: June 2022

Introduction. Olaparib is the only PARP inhibitor approved in Russia for the maintenance therapy for BRCA-positive ovarian cancer after frontline chemotherapy. We conducted a real-world analysis of olaparib efficacy and safety for this indication.

Aim. To assess the efficacy of PARP inhibitors in real-world clinical practice.

Materials and methods. Patients with stage III-IV BRCA-mutated ovarian cancer who received olaparib maintenance therapy in expanded access program in 03.2019-12.2020 timeframe. Briefly, key inclusion criteria were: serous or endometrioid highgrade ovarian cancer; pathogenic BRCA1/2 mutation; primary or interval debulking; complete or partial response to frontline platinum-based therapy. Olaparib was administered as tablets (300 mg BID) up to 2-years, disease progression or unacceptable toxicity, whichever occurred first. The primary endpoint of the study was progression-free survival (PFS), overall survival (OS) and safety were key secondary endpoints. Statistical analysis was done with R and RStudio software.

Results and discussion. 23 patients were enrolled. Median age was 49 years, all patients had high-grade serous adenocarcinoma histology. Complete debulking at primary or interval surgery was achieved in 30% of patients. With median follow-up time equal to 22 months, median PFS and OS were not reached. The 2-year PFS and OS were 65 and 84%, respectively. Grade 3-4 adverse events was detected in 7 (30.4%) patients.

Conclusion. Our results supports high efficacy of olaparib in real clinical practice setting reported in the SOLO1 trial.
Second line therapy for metastatic urothelial carcinoma. Clinical case of vinflunine use
Authors: Gritskevich A. A. , Popov A. Yu. , Rusakov I. G. , Vozniy E. K. , Makarov V. A. , Gridnev D. I. , Baitman T. P. , Mishugin S. V. , Novikova I. V. , Khromova S. V.
Published: June 2022

Urothelial cancer occupies a significant place in the routine practice of cancer treatment. Systemic antitumor treatment of patients with metastatic urothelial cancer in the first line is currently well studied, has its own standards, implemented in clinical practice. However, the problem of choosing antitumor treatment for patients with metastatic urothelial cancer in the second line remains relevant. Vinflunine is one of the treatment options for such patients. This article presents the case of successful treatment of metastatic urothelial cancer in the second line with vinflunine. A 63-year-old patient with a diagnosis of C65 Urothelial cancer of the pelvis of the left kidney T3N0M1, stage IV, bone metastases. Condition after cytoreductive left-sided nephrectomy, para-aortic lymphadenectomy from 04/16/2021. Concomitant pathology: Anemia. Diabetes mellitus type 2 Hypertonic disease. As the 1st line of treatment, 6 courses of CT were performed according to the scheme: Cisplatin + Gemcitabine. The effect was evaluated according to MSCT data of 3 zones and bone scintigraphy in accordance with Recist 1.1. The best response was obtained after the 4th cycle in August 2021 in the form of stabilization. October 2021 follow-up examination revealed progression. Since October 2021, chemotherapy of the 2nd line with Vinflunin is carried out in mono mode. All AEs are well controlled and do not require discontinuation of the drug. According to the results of the control examination, stabilization was achieved. Thereby vinflunine has been shown to be effective as a second-line treatment for platinum-resistant recurrent urothelial cancer.
Polyvinyl Alcohol can Stabilize FITC Conjugated Recombinant Annexin V
for Apoptotic Cells Detection
Authors: Shobeiri Saeideh Sadat , Sankian Mojtaba
Published: June 2022

Background:

Annexin V, a member of calcium-dependent phospholipid-binding proteins, selectively binds to the exposed phosphatidylserine, which can be used for in vitro apoptosis detection. Simultaneous staining of cells with annexin V-fluorescein isothiocyanate (FITC) and the non-vital dye propidium iodide (PI) enables detection of apoptotic and necrotic cells.

Objective:

Our study aimed to express, purify, and stabilize the recombinant annexin V.

Method:

The recombinant annexin V was cloned and expressed in E. coli bacteria and was purified using Ni-IDA resin. The FITC conjugation was performed, and apoptosis detection of HaCaT cells by FITC-labeled annexin V was evaluated by flow cytometry. Then, the stability of FITC-labeled annexin in various conditions, including polyvinyl alcohol (PVA), glycerol, and trehalose, was evaluated.

Results:

The results showed that annexin V was appropriately expressed and purified. After FITC conjugation, it could perfectly detect the cell death of HaCat cells in different apoptosis percentages. FITC-labeled annexin had more stability with PVA than glycerol and trehalose.

Conclusion:

It seems that PVA has an acceptable effect on FITC-labeled annexin V stability in concentrations lower than 1 mg mL-1, without interfering in fluorescent intensity.
Sodium-glucose Cotransporter Type 2 Inhibitors: A New Insight into the Molecular
Mechanisms of Diabetic Nephropathy
Authors: Li Na , Zhou Hong
Published: June 2022

Diabetic nephropathy is one of the chronic microvascular complications of diabetes and is a leading
cause of end-stage renal disease. Fortunately, clinical trials have demonstrated that sodium-glucose cotransporter
type 2 inhibitors could decrease proteinuria and improve renal endpoints and are promising agents for the
treatment of diabetic nephropathy. The renoprotective effects of sodium-glucose cotransporter type 2 inhibitors
cannot be simply attributed to their advantages in aspects of metabolic benefits, such as glycemic control, lowering
blood pressure, and control of serum uric acid, or improving hemodynamics associated with decreased
glomerular filtration pressure. Some preclinical evidence suggests that sodium-glucose cotransporter type 2 inhibitors
exert their renoprotective effects by multiple mechanisms, including attenuation of oxidative and endoplasmic
reticulum stresses, anti-fibrosis and anti-inflammation, protection of podocytes, suppression of megalin
function, improvement of renal hypoxia, restored mitochondrial dysfunction and autophagy, as well as inhibition
of sodium-hydrogen exchanger 3. In the present study, the detailed molecular mechanisms of sodiumglucose
cotransporter type 2 inhibitors with the actions of diabetic nephropathy were reviewed, with the purpose
of providing the basis for drug selection for the treatment of diabetic nephropathy.
Neoadjuvant Endocrine Therapy: A Potential Way to Make Cold
Hormone Receptor-Rich Breast Cancer Hot
Authors: Zhang Suzhan , Hu Yue , Hu Kaimin , Wu Yinan , Gong Xiaonan , Wang Kaiyue , Yu Chengcheng , Qiu Jili
Published: June 2022

Background:

Turning the “cold” tumor immune microenvironment into “hot” is a critical
issue in cancer treatment today. Hormone receptor-rich breast cancer (HR+ BC) was previously
considered immunologically quiescent.

Objective:

This study aims to explore the immunomodulatory effects of endocrine therapy on HR+
BCs.

Methods:

The infiltrations and alterations of the tumor immune microenvironment in HR+ BCs before,
after 10-14 days, and after three months of neoadjuvant endocrine therapy were computationally
analyzed according to MCP-counter, CIBERSORT, xCell algorithms, and gene-set enrichment
analysis (GSEA). The primary microarray data were obtained from three HR+ BC gene expression
datasets (GSE20181, GSE55374, and GSE59515). Single-sample GSEA of hallmark and immune
response gene sets was performed to evaluate the correlation between suspected treatment response
and activated immune pathways in tumors.

Results:

Both immune and stromal cells were specifically recruited into the HR+ BCs who responded
to the neoadjuvant endocrine therapy by letrozole. Besides the enhanced infiltrations of
immunosurveillance-related cells such as CD8+ T cells, dendritic cells, and the activation of immune
response-related signals, the immunosuppressive M2-like macrophages, as well as the expression
of immune checkpoint genes like PDCD1, SIRPA, and some HLA genes, were also stimulated
in responders. We identified four pretreatment indicators (the intrinsic luminal subtype, the
estrogen response early/late pathway, and the epithelial-mesenchymal transition pathway) as
potential predictors of both clinical response and the activation of the tumor immune microenvironment
post letrozole.

Conclusions:

Neoadjuvant endocrine therapy showed a promising way to convert the immunologically
“cold” HR+ BCs into “hot” tumors. This study provides new insights into the application of
immunotherapy for HR+ BCs, especially those who respond to endocrine therapy.
Adult-onset and Juvenile-onset Still’s Disease: A Comparative Study of
Both Sides
Authors: Aicha Ben Tekaya , Emna Hannech , Olfa Saidane , Selma Bouden , Leila Rouached , Rawda Tekaya , Ines Mahmoud , Slim Abdelmoula , Leila Abdelmoula
Published: June 2022

Background:

Adult-onset Still’s disease and systemic-onset juvenile idiopathic arthritis constitute two sides of the same continuum disease. We aimed to investigate the similarities and dif-ferences between those diseases.

Methods:

We conducted a retrospective study including adult patients affected by still’s disease, at-tending the rheumatology department and patients affected by systemic-onset juvenile idiopathic arthritis attending the pediatric department. We recorded clinical and radiological findings, different therapeutic regimens, and disease patterns.

Results:

There were 8 adult patients (6 females and 2 males) and 8 juvenile patients (4 females and 4 males). The classical triad of spiking fever, arthritis, and evanescent skin rash was the first clinical presentation observed in 4 adult patients and in 2 juvenile patients. Arthritis was noted in 8 adult pa-tients versus 6 juvenile patients. Joint deformities were seen in adult patients. Non-steroid anti-inflammatory drugs and corticosteroids were the most prescribed molecules. csDMARDs and bDMARDs were used in second-line therapy only for adult patients. The monocyclic course was predominant in juvenile patients and the polycyclic course in adult patients. The chronic course was observed only in two adult patients. Remission was noted in 5 adult patients and 6 juvenile patients. There were no significant differences between the two groups regarding clinical findings, different therapeutic regimens, and disease patterns.

Conclusion:

From the findings of our study, it seems that AOSD and sJIA are the same syndrome continuum expressed in different hosts. This hypothesis is supported by clinical course, molecule evidence, cytokine profile, and treatment response.
Neurally Mediated Syncope in Children and Adolescents: An Updated Narrative Review
Authors: Lisboa da Silva Rose Mary Ferreira , Oliveira Pamela Michelle Leite , Tonelli Henrique Assis Fonseca , Alves Meira Zilda Maria , Mota Cleonice de Carvalho Coelho
Published: June 2022

Syncope presents a proportion of up to 25% among children and adolescents and accounts for 0.9% of emergency room visits. Its most frequent aetiology is neurally mediated syncope, which includes vasovagal syncope and orthostatic hypotension. Up to 70% of the paediatric population with reflex syncope is female. There are usually precipitating factors and prodromes. This mini-review will discuss the particularities of the clinical presentation, diagnosis, modified Calgary score, indications and accuracy of the head-up tilt test, classification, and pathophysiology in four sequential phases. Prognostic data and the non-pharmacological and pharmacological approaches will also be reviewed and differences regarding reflex syncope in adults will be discussed.
Colored Edge Detection Using Thresholding Techniques
Authors: Fenyi Adolf , Fenyi Isaac , Asante Michael
Published: June 2022

Background:

In this research, a novel algorithm is formulated through the combination of gradient
and adaptive thresholding. A set of 5 X 5 convolution kernels were generated to determine the gradients in the
four main directions of the image

Objectives:

The researcher converted the gaussian equation into a normalized kernel, which was convolved
with the gradients to suppress the impact of noise

Methods:

The edges derived were partitioned into a set of 5 x 5 matrices. A weighted variance was calculated
for each local window in the image. The pixel that generated the minimum variance was used for the
segmentation process in each local window. The researcher then trimmed multiple pixel width edges into
singles by developing a set of 5 X 5 Structuring Elements (SE). These elements were placed over the image to
remove boundary pixels. In order to produce colored edges, the algorithm was executed over all the channels
and the results were concatenated to produce the skeletal colored edges.

Results:

From the evaluations conducted, the proposed algorithm exhibited better performance than most of
the recent algorithms with respect to Human Perception Clarity and time complexity in both noisy and nonuniform illuminated images.

Conclusion:

The reason for this performance is that it is able to extract edges moving in the various directions
of images. It also ensures that identified edges are single pixel width instead of multiple.
Radices 1 - Volledig-Complet
Authors: Outreach OCAD/OCAM
Published: June 2022

Radices 1 - Volledig-Complet
COVID-box Experiences of Patients and Health Care Professionals (COVID-box Project): Single-Center, Retrospective, Observational Study
Authors: Dirikgil Ebru , Brons Kim , Duindam Michael , Groeneveld Geert H , Geelhoed J J Miranda , Heringhaus Christian , van der Boog Paul J M , Rabelink Ton J , Bos Willem Jan W , Chavannes Niels H , Atsma Douwe E , Teng Y K Onno
Published: June 2022

Background

During the COVID-19 pandemic, several home monitoring programs have described the success of reducing hospital admissions, but only a few studies have investigated the experiences of patients and health care professionals.

Objective

The objective of our study was to determine patients’ and health care professionals’ experiences and satisfaction with employing the COVID-box.

Methods

In this single-center, retrospective, observational study, patients and health care professionals were asked to anonymously fill out multiple-choice questionnaires with questions on a 5-point or 10-point Likert scale. The themes addressed by patients were the sense of reassurance and safety, experiences with teleconsultations, their appreciation for staying at home, and the instructions for using the COVID-box. The themes addressed by health care professionals who treated patients with the COVID-box were the characteristics of the COVID-box, the technical support service and general satisfaction, and their expectations and support for this telemonitoring concept. Scores were interpreted as insufficient (≤2 or ≤5, respectively), sufficient (3 or 6-7, respectively), or good (≥4 or ≥8, respectively) on a 5-point or 10-point Likert scale.

Results

A total of 117 patients and 25 health care professionals filled out the questionnaires. The median score was 4 (IQR 4-5) for the sense of safety, the appreciation for staying at home, and experiences with teleconsultations, with good scores from 76.5% (88/115), 86% (56/65), and 83.6% (92/110) of the patients, respectively. Further, 74.4% (87/117) of the patients scored the home monitoring program with a score of ≥8. Health care professionals scored the COVID-box with a minimum median score of 7 (IQR 7-10) on a 10-point scale for all domains (ie, the characteristics of the COVID-box and the technical support service and general satisfaction). For the sense of safety, user-friendliness, and additional value of the COVID-box, the median scores were 8 (IQR 8-10), 8 (IQR 7-9), and 10 (IQR 8-10), respectively, with good scores from 86% (19/22), 75% (15/20), and 96% (24/25) of the health care professionals, respectively. All health care professionals (25/25, 100%) gave a score of ≥8 for supporting this home monitoring concept, with a median score of 10 (IQR 10-10).

Conclusions

The positive experiences and satisfaction of involved users are key factors for the successful implementation of a novel eHealth solution. In our study, patients, as well as health care professionals, were highly satisfied with the use of the home monitoring program—the COVID-box project. Remote home monitoring may be an effective approach in cases of increased demand for hospital care and high pressure on health care systems.
Vaccination Intention and Behavior of the General Public in China: Cross-sectional Survey and Moderated Mediation Model Analysis (Preprint)
Authors: Yang Liuqing , Ji Lili , Wang Qiang , Xu Yan , Yang Guoping , Cui Tingting , Shi Naiyang , Zhu Lin , Xiu Shixin , Jin Hui , Zhen Shiqi
Published: June 2022

BACKGROUND

Promoting vaccination and eliminating vaccine hesitancy are key measures for controlling vaccine-preventable diseases.

OBJECTIVE

We aimed to understand the beliefs surrounding and drivers of vaccination behavior, and their relationships with and influence on vaccination intention and practices.

METHODS

We conducted a web-based survey in 31 provinces in mainland China from May 24, 2021 to June 15, 2021, with questions pertaining to vaccination in 5 dimensions: attitude, subjective norms, perceived behavioral control, intention, and behavior. We performed hierarchical regression analysis and structural equation modeling based on the theory of planned behavior—in which, the variables attitude, subjective norms, and intention each affect the variable intention; the variable intention mediates the relationships of attitude and subjective norms with behavior, and the variable perceived behavioral control moderates the strength of this mediation—to test the validity of the theoretical framework.

RESULTS

A total of 9924 participants, aged 18 to 59 years, were included in this study. Vaccination intention mediated the relationships of attitude and subjective norms with vaccination behavior. The indirect effect of attitude on vaccination behavior was 0.164 and that of subjective norms was 0.255, and the difference was statistically significant (P<.001). The moderated mediation analysis further indicated that perceived behavioral control would affect the mediation when used as moderator, and the interaction terms for attitude (β=–0.052, P<.001) and subjective norms (β=–0.028, P=.006) with perceived behavioral control were significant.

CONCLUSIONS

Subjective norms have stronger positive influences on vaccination practices than attitudes. Perceived behavioral control, as a moderator, has a substitution relationship with attitudes and subjective norms and weakens their positive effects on vaccination behavior.