bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling Segregation of Fe–C Alloy in Solidification by Phase-Field Method Coupled with Thermodynamics
Authors: Gong Tong-Zhao , Chen Yun , Hao Wei-Ye , Chen Xing-Qiu , Li Dian-Zhong
Published: June 2023

The primary carbide in high carbon chromium bearing steels, which arises from solute segregation during non-equilibrium solidification, is one of the key factors affecting the mechanical properties and performance of the related components. In this work, the effects of carbide forming element diffusion, primary austenite grain size, and the cooling rate on solute segregation and carbide precipitation during the solidification of an Fe–C binary alloy were studied by the phase-field method coupled with a thermodynamic database. It was clarified that increasing the ratio of solute diffusivity in solid and liquid, refining the grain size of primary austenite to lower than a critical value, and increasing the cooling rate can reduce the solute segregation and precipitation of primary carbide at late solidification. Two characteristic parameters were introduced to quantitatively evaluate the solute segregation during solidification including the phase fraction threshold of primary austenite when the solute concentration in liquid reaches the eutectic composition, and the maximum segregation ratio. Both parameters can be well-correlated to the ratio of solute diffusivity in solid and liquid, the grain size of primary austenite, and the cooling rate, which provides potential ways to control the solute segregation and precipitation of primary carbide in bearing steels.
Metal–Phenolic Film Coated Quartz Crystal Microbalance as a Selective Sensor for Methanol Detection in Alcoholic Beverages
Authors: Esmeryan Karekin D. , Lazarov Yuliyan , Grakov Teodor , Fedchenko Yulian I. , Vergov Lazar G. , Staykov Stefan
Published: June 2023

The facile real-time monitoring of methyl content in fermented beverages is of fundamental significance in the alcohol and restaurant industry, since as little as 4 mL of methanol entering the blood may cause intoxication or blindness. So far, the practical applicability of available methanol sensors, including the piezoresonance analogs, is somewhat limited to laboratory use due to the complexity and bulkiness of the measuring equipment involving multistep procedures. This article introduces a hydrophobic metal–phenolic film-coated quartz crystal microbalance (MPF-QCM) as a novel streamlined detector of methanol in alcoholic drinks. Unlike other QCM-based alcohol sensors, our device operates under saturated vapor pressure conditions, permitting rapid detection of methyl fractions up to seven times below the tolerable levels in spirits (e.g., whisky) while effectively suppressing the cross-sensitivity to interfering chemical compounds such as water, petroleum ether or ammonium hydroxide. Furthermore, the good surface adhesion of metal–phenolic complexes endows the MPF-QCM with superior long-term stability, contributing to the repeatable and reversible physical sorption of the target analytes. These features, combined with the lack of mass flow controllers, valves and connecting pipes delivering the gas mixture, outline the likelihood for future design of a portable MPF-QCM prototype suitable to point-of-use analysis in drinking establishments.
A Ku-Band Broadband Stacked FET Power Amplifier Using 0.15 μm GaAs pHEMT
Authors: Li Jiaxuan , Yuan Yang , Yuan Bin , Fan Jingxin , Zeng Jialong , Yu Zhongjun
Published: June 2023

To meet the application requirements of broadband radar systems for broadband power amplifiers, a Ku-band broadband power amplifier (PA) microwave monolithic integrated circuit (MMIC) based on a 0.15 µm gallium arsenide (GaAs) high-electron-mobility transistor (HEMT) technology is proposed in this paper. In this design, the advantages of the stacked FET structure in the broadband PA design are illustrated by theoretical derivation. The proposed PA uses a two-stage amplifier structure and a two-way power synthesis structure to achieve high-power gain and high-power design, respectively. The fabricated power amplifier was tested under continuous wave conditions, and the test results showed a peak power of 30.8 dBm at 16 GHz. At 15 to 17.5 GHz, the output power was above 30 dBm with a PAE of more than 32%. The fractional bandwidth of the 3 dB output power was 30%. The chip area was 3.3 × 1.2 mm2 and included input and output test pads.
Petrology and Age of the Yamaat Uul Mafic Complex, Khangai Mountains, Western Mongolia
Authors: Shelepaev Roman , Shapovalova Maria , Egorova Vera , Shelepov Yaroslav , Oyunchimeg Tumen-Ulzii , Tolstykh Nadezhda
Published: June 2023

The Yamaat Uul mafic complex with Cu-Ni mineralization is located in the Khangai Mountains of Western Mongolia. We have received new unique data for mafic rocks of the complex: U-Pb dating (SHRIMP II), mineralogy (WDS) and geochemistry (XRF, ICP-MS), Sm-Nd and Rb-Sr isotope data and sulphur isotopes. The Yamaat Uul mafic complex consists of two intrusions: Intrusion 1 is represented by rocks of plagioclase cumulates and olivine–pyroxene cumulates; Intrusion 2 consists of monzogabbro. Intrusions 1 and 2 are different in composition of minerals such as olivine, plagioclase and biotite. The monzogabbro has higher contents of incompatible elements (REE, K, Ti, P) than rocks of Intrusion 1. Zircon U-Pb dating of the anorthosite and Bt-Am-Ol gabbronorite shows a Late Permian age (255.8 ± 2.9 Ma and 262.6 ± 3.1 Ma, respectively) for the Yamaat Uul mafic complex. All of the rocks of the complex are derived from a unified parental melt due to different amounts of trapped melts in plagioclase and olivine–pyroxene cumulates and without crustal contamination. The Cu-Ni mineralization of the complex has a low degree of evolution of the sulphide melt, similar to PGE-Cu-Ni mafic–ultramafic intrusions of the Khangai Mountains (Nomgon and Oortsog Uul). The Yamaat Uul mafic complex together with other mafic–ultramafic intrusions of the Khangai Mountains is related to the Khangai LIP and can be considered as potential for the PGE-Cu-Ni. The new geological, petrological, geochemical and isotope–geochronological data can later be used to reconstruct the geotectonics of the Khangai Mountains and the Central Asian orogenic belt as a whole.
On the Characterization of Viscoelastic Parameters of Polymeric Pipes for Transient Flow Analysis
Authors: Pezzinga Giuseppe
Published: June 2023

The behaviour of polymeric pipes in transient flows has been proved to be viscoelastic. Generalized Kelvin–Voigt (GKV) models perform very well when simulating the experimental pressure. However, in the literature, no general indications on the evaluation of the model parameters are given. In the present study, the calibration of GKV model parameters is carried out using a micro-genetic algorithm for experimental tests of transient flows in polymeric pipes taken from the literature. The results confirm that the higher the number of Kelvin–Voigt elements, the better the reproduction of experimental tests, but it is difficult to search for general rules for parameter characterization. Assuming a Kelvin–Voigt (KV) model with a single element, it is shown that the retardation time is related to the oscillation period that can be obtained from the elastic modulus and from easily evaluable pipe characteristics. A simple procedure is then proposed for the characterization of the viscoelastic parameters that can be used by manufacturers and technicians. Considering the limits of such a model, the procedure has to be considered as a first step for the characterization of the viscoelastic parameters of more complex models.
Exploring Proton-Only NMR Experiments and Filters for Daphnia In Vivo: Potential and Limitations
Authors: Ronda Kiera , Downey Katelyn , Jenne Amy , Bastawrous Monica , Wolff William W. , Steiner Katrina , Lysak Daniel H. , Costa Peter M. , Simpson Myrna J. , Jobst Karl J. , Simpson Andre J.
Published: June 2023

Environmental metabolomics provides insight into how anthropogenic activities have an impact on the health of an organism at the molecular level. Within this field, in vivo NMR stands out as a powerful tool for monitoring real-time changes in an organism’s metabolome. Typically, these studies use 2D 13C-1H experiments on 13C-enriched organisms. Daphnia are the most studied species, given their widespread use in toxicity testing. However, with COVID-19 and other geopolitical factors, the cost of isotope enrichment increased ~6–7 fold over the last two years, making 13C-enriched cultures difficult to maintain. Thus, it is essential to revisit proton-only in vivo NMR and ask, “Can any metabolic information be obtained from Daphnia using proton-only experiments?”. Two samples are considered here: living and whole reswollen organisms. A range of filters are tested, including relaxation, lipid suppression, multiple-quantum, J-coupling suppression, 2D 1H-1H experiments, selective experiments, and those exploiting intermolecular single-quantum coherence. While most filters improve the ex vivo spectra, only the most complex filters succeed in vivo. If non-enriched organisms must be used, then, DREAMTIME is recommended for targeted monitoring, while IP-iSQC was the only experiment that allowed non-targeted metabolite identification in vivo. This paper is critically important as it documents not just the experiments that succeed in vivo but also those that fail and demonstrates first-hand the difficulties associated with proton-only in vivo NMR.
The Effect of Mineral Fertilization on the Content of Bioactive Compounds in Hemp Seeds and Oil
Authors: Frankowski Jakub , Przybylska-Balcerek Anna , Graczyk Małgorzata , Niedziela Grażyna , Sieracka Dominika , Stuper-Szablewska Kinga
Published: June 2023

The popularity of hemp cultivation for industrial purposes has been steadily growing for many years. With the addition of products derived from these plants to the Novel Food Catalogue, maintained by the European Commission, a significant increase in interest in hemp food is also expected. The aim of the study was to determine the characteristics of hempseed, oil, and oil cake samples produced from experimental plots grown in different conditions. The research was conducted on the Henola variety, one of the newest and most popular varieties of hemp, recently bred for grain and oil. The content of bioactive compounds in grain and oil has been subjected to detailed chemical analyses in order to determine the effect of fertilization, the method of plant cultivation, and processing conditions on their quantity. The test results and the statistical analysis carried out showed a significant impact of the tested factors on the content of some of the tested bioactive compounds. The obtained results will help in the development of an effective method of cultivation for this hemp variety in order to maximize the content of the desired bioactive compounds per unit of cultivation area.
Micronized Powder of Raspberry Pomace as a Source of Bioactive Compounds
Authors: Różyło Renata , Amarowicz Ryszard , Janiak Michał Adam , Domin Marek , Gawłowski Sławomir , Kulig Ryszard , Łysiak Grzegorz , Rząd Klaudia , Matwijczuk Arkadiusz
Published: June 2023

Red raspberries, which contain a variety of nutrients and phytochemicals that are beneficial for human health, can be utilized as a raw material in the creation of several supplements. This research suggests micronized powder of raspberry pomace production. The molecular characteristics (FTIR), sugar, and biological potential (phenolic compounds and antioxidant activity) of micronized raspberry powders were investigated. FTIR spectroscopy results revealed spectral changes in the ranges with maxima at ~1720, 1635, and 1326, as well as intensity changes in practically the entire spectral range analyzed. The discrepancies clearly indicate that the micronization of the raspberry byproduct samples cleaved the intramolecular hydrogen bonds in the polysaccharides present in the samples, thus increasing the respective content of simple saccharides. In comparison to the control powders, more glucose and fructose were recovered from the micronized samples of the raspberry powders. The study’s micronized powders were found to contain nine different types of phenolic compounds, including rutin, elagic acid derivatives, cyanidin-3-sophoroside, cyanidin-3-(2-glucosylrutinoside), cyanidin-3-rutinoside, pelargonidin-3-rutinoside, and elagic acid derivatives. Significantly higher concentrations of ellagic acid and ellagic acid derivatives and rutin were found in the micronized samples than in the control sample. The antioxidant potential assessed by ABTS and FRAP significantly increased following the micronization procedure.
Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase
Authors: Malric-Garajova Sona , Fortuna Florian , Pion Florian , Martin Elise , Thottathil Adithya , Guillemain Audrey , Doan Annick , Lomascolo Anne , Faulds Craig , Baumberger Stéphanie , Foulon Laurence , Chabbert Brigitte , de Baynast Hélène , Dubessay Pascal , Audonnet Fabrice , Bertrand Emmanuel , Sciara Giuliano , Tapin-Lingua Sandra , Ducrot Paul-Henri , Michaud Philippe , Aguié-Béghin Véronique , Record Eric
Published: June 2023

Here, we report work on developing an enzymatic process to improve the functionalities of industrial lignin. A kraft lignin sample prepared from marine pine was treated with the high-redox-potential laccase from the basidiomycete fungus Pycnoporus cinnabarinus at three different concentrations and pH conditions, and with and without the chemical mediator 1-hydroxybenzotriazole (HBT). Laccase activity was tested in the presence and absence of kraft lignin. The optimum pH of PciLac was initially 4.0 in the presence and absence of lignin, but at incubation times over 6 h, higher activities were found at pH 4.5 in the presence of lignin. Structural changes in lignin were investigated by Fourier-transform infrared spectroscopy (FTIR) with differential scanning calorimetry (DSC), and solvent-extractable fractions were analyzed using high-performance size-exclusion chromatography (HPSEC) and gas chromatography–mass spectrometry (GC–MS). The FTIR spectral data were analyzed with two successive multivariate series using principal component analysis (PCA) and ANOVA statistical analysis to identify the best conditions for the largest range of chemical modifications. DSC combined with modulated DSC (MDSC) revealed that the greatest effect on glass transition temperature (Tg) was obtained at 130 U g cm−1 and pH 4.5, with the laccase alone or combined with HBT. HPSEC data suggested that the laccase treatments led to concomitant phenomena of oligomerization and depolymerization, and GC–MS revealed that the reactivity of the extractable phenolic monomers depended on the conditions tested. This study demonstrates that P. cinnabarinus laccase can be used to modify marine pine kraft lignin, and that the set of analytical methods implemented here provides a valuable tool for screening enzymatic treatment conditions.
Amino Acid-Coated Zeolitic Imidazolate Framework for Delivery of Genetic Material in Prostate Cancer Cell
Authors: Polash Shakil Ahmed , Garlick-Trease Koen , Pyreddy Suneela , Periasamy Selvakannan , Bryant Gary , Shukla Ravi
Published: June 2023

Metal–organic frameworks (MOFs) are currently under progressive development as a tool for non-viral biomolecule delivery. Biomolecules such as proteins, lipids, carbohydrates, and nucleic acids can be encapsulated in MOFs for therapeutic purposes. The favorable physicochemical properties of MOFs make them an attractive choice for delivering a wide range of biomolecules including nucleic acids. Herein, a green fluorescence protein (GFP)-expressing plasmid DNA (pDNA) is used as a representative of a biomolecule to encapsulate within a Zn-based metal–organic framework (MOF) called a zeolitic imidazolate framework (ZIF). The synthesized biocomposites are coated with positively charged amino acids (AA) to understand the effect of surface functionalization on the delivery of pDNA to prostate cancer (PC-3) cells. FTIR and zeta potential confirm the successful preparation of positively charged amino acid-functionalized derivatives of pDNA@ZIF (i.e., pDNA@ZIFAA). Moreover, XRD and SEM data show that the functionalized derivates retain the pristine crystallinity and morphology of pDNA@ZIF. The coated biocomposites provide enhanced uptake of genetic material by PC-3 human prostate cancer cells. The AA-modulated fine-tuning of the surface charge of biocomposites results in better interaction with the cell membrane and enhances cellular uptake. These results suggest that pDNA@ZIFAA can be a promising alternative tool for non-viral gene delivery.
Anticancer Activity and Mode of Action of Cu(II), Zn(II), and Mn(II) Complexes with 5-Chloro-2-N-(2-quinolylmethylene)aminophenol
Authors: Gai Shuangshuang , He Liqin , He Mingxian , Zhong Xuwei , Jiang Caiyun , Qin Yiming , Jiang Ming
Published: June 2023

Developing a new generation of anticancer metal-based drugs that can both kill tumor cells and inhibit cell migration is a promising strategy. Herein, we synthesized three Cu(II), Zn(II), and Mn(II) complexes derived from 5-chloro-2-N-(2-quinolylmethylene)aminophenol (C1–C3). Among these complexes, the Cu(II) complex (C1) showed significantly greater cytotoxicity toward lung cancer cell lines than cisplatin. C1 inhibited A549 cell metastasis and suppressed the growth of the A549 tumor in vivo. In addition, we confirmed the anticancer mechanism of C1 by triggering multiple mechanisms, including inducing mitochondrial apoptosis, acting on DNA, blocking cell cycle arrest, inducing cell senescence, and inducing DNA damage.
Chemical Constituents from the Fruits of Amomum kravanh and Their Role in Activating Alcohol Dehydrogenase
Authors: Xiong Hao-Ming , Li Hui-Ying , Lin Zhi-Rong , Liu Xiao-Mei , Bai Li-Ping , Zhang Wei , Jiang Zhi-Hong , Zhu Guo-Yuan
Published: June 2023

Alcoholism is a worldwide health problem, and diseases caused by alcoholism are killing people every year. Amomum kravanh is a traditional Chinese medicine used to relieve hangovers. However, whether its bioactive components improve alcohol metabolism is not clear. In this study, ten new (amomumols A-J, 1–10) and thirty-five known (11–45) compounds were isolated from the fruits of Amomum kravanh by an activity-guided separation. Ten novel compounds were identified as four sesquiterpenoids (1–4), three monoterpene derivatives (5–7), two neolignans (8, 9), and a novel norsesquiterpenoid (10) with a new C14 nor-bisabolane skeleton. Their structures were determined by the comprehensive analysis of high-resolution electrospray ionization mass spectrometry (HRESIMS), nuclear magnetic resonance (NMR), and electronic circular dichroism (ECD) calculation. The effects of all isolated compounds on the activity of alcohol dehydrogenase were evaluated in vitro, and it was found that eight compounds (11, 12, 15, 18, 26, and 36–38) exhibited significant activation effects on the alcohol dehydrogenase at 50 μM.
Eu(III) and Cm(III) Complexation by the Aminocarboxylates NTA, EDTA, and EGTA Studied with NMR, TRLFS, and ITC—An Improved Approach to More Robust Thermodynamics
Authors: Friedrich Sebastian , Sieber Claudia , Drobot Björn , Tsushima Satoru , Barkleit Astrid , Schmeide Katja , Stumpf Thorsten , Kretzschmar Jerome
Published: June 2023

The complex formation of Eu(III) and Cm(III) was studied via tetradentate, hexadentate, and octadentate coordinating ligands of the aminopolycarboxylate family, viz., nitrilotriacetate (NTA3−), ethylenediaminetetraacetate (EDTA4−), and ethylene glycol-bis(2-aminoethyl ether)-N,N,N′,N′-tetraacetate (EGTA4−), respectively. Based on the complexones’ pKa values obtained from 1H nuclear magnetic resonance (NMR) spectroscopic pH titration, complex formation constants were determined by means of the parallel-factor-analysis-assisted evaluation of Eu(III) and Cm(III) time-resolved laser-induced fluorescence spectroscopy (TRLFS). This was complemented by isothermal titration calorimetry (ITC), providing the enthalpy and entropy of the complex formation. This allowed us to obtain genuine species along with their molecular structures and corresponding reliable thermodynamic data. The three investigated complexones formed 1:1 complexes with both Eu(III) and Cm(III). Besides the established Eu(III)–NTA 1:1 and 1:2 complexes, we observed, for the first time, the existence of a Eu(III)–NTA 2:2 complex of millimolar metal and ligand concentrations. Demonstrated for thermodynamic studies on Eu(III) and Cm(III) interaction with complexones, the utilized approach is commonly applicable to many other metal–ligand systems, even to high-affinity ligands.
Synthesis and Characterization of Iron Bispyridine Bisdicyanamide, Fe[C5H5N]2[N(CN)2]2
Authors: Henrich Laura , Müller Peter C. , Hempelmann Jan , Mann Markus , van Leusen Jan , Steinberg Simon , Dronskowski Richard
Published: June 2023

Fe[C5H5N]2[N(CN)2]2 (1) was synthesized from a reaction of stoichiometric amounts of NaN(CN)2 and FeCl2·4H2O in a methanol/pyridine solution. Single-crystal and powder diffraction show that 1 crystallizes in the monoclinic space group I2/m (no. 12), different from Mn[C5H5N]2[N(CN)2]2 (P21/c, no. 14) due to tilted pyridine rings, with a = 7.453(7) Å, b = 13.167(13) Å, c = 8.522(6) Å, β = 114.98(6)° and Z = 2. ATR-IR, AAS, and CHN measurements confirm the presence of dicyanamide and pyridine. Thermogravimetric analysis shows that π-stacking interactions of the pyridine rings play an important role in structural stabilization. Based on DFT-optimized structures, a chemical bonding analysis was performed using a local-orbital framework by projection from a plane-wave basis. The resulting bond orders and atomic charges are in good agreement with the expectations based on the structure analysis. SQUID magnetic susceptibility measurements show a high-spin state FeII compound with predominantly antiferromagnetic exchange interactions at lower temperatures.
Comparison of Homo-Polyimide Films Derived from Two Isomeric Bis-Benzimidazole Diamines
Authors: Lian Meng , Zheng Feng , Meng Lingbin , Zhao Fei , Liu Jun , Song Jimei , Lu Qinghua
Published: June 2023

Heteroaromatic polyimides (PIs) containing benzimidazole have attracted tremendous attention due to their positive impact on the properties of PIs. Some research on PIs containing 4,4′-[5,5′-bi-1H-benzimidazole]-2,2′-diylbis-benzenamine (4-AB) has been reported. However, reports are lacking on homo-polyimides (homo-PIs) containing 3,3′-[5,5′-bi-1H-benzimidazole]-2,2′-diylbis-benzenamine (3-AB), which is one of the isomers of 4-AB. In this paper, the influence of amino groups’ positions on the performance of homo-PIs was investigated. It was found that the net charge of the amine N group in 4-AB was lower than that of 3-AB, resulting in higher reactivity of 4-AB. Consequently, PIs containing 4-AB displayed better mechanical performance. Molecular simulation confirmed that 3-AB and its corresponding PI chain exhibited distorted conformation, leading to the PI films containing 3-AB having a lighter color. In addition, the 3-AB structure was calculated to have higher rotational energy compared to 4-AB, resulting in a higher glass transition temperature (Tg) in PIs prepared from 3-AB. On the other hand, PIs containing 4-AB exhibited a higher level of molecular linearity, leading to a lower coefficient of thermal expansion (CTE) compared to PIs prepared from 3-AB. Furthermore, all PIs showed higher thermal stability with a 5% weight loss temperature above 530 °C and Tg higher than 400 °C.
The Role of Vitamin B6 in Peripheral Neuropathy: A Systematic Review
Authors: Muhamad Raman , Akrivaki Alexandra , Papagiannopoulou Georgia , Zavridis Periklis , Zis Panagiotis
Published: June 2023

Introduction: Vitamin B6 is a water-soluble vitamin that is naturally present in many foods and is accessible in many dietary supplements. The three natural forms are pyridoxine, pyridoxal, and pyridoxamine. Both vitamin B6 deficiency and high B6 intake have been described as risk factors for developing peripheral neuropathy (PN). The aim of this systematic review is to characterize and comprehensively describe B6-related PN. Method: A systematic, computer-based search was conducted using the PubMed database. Twenty articles were included in this review. Results: Higher vitamin B6 levels, which usually occur following the taking of nutritional supplements, may lead to the development of a predominantly, if not exclusively, sensory neuropathy of the axonal type. After pyridoxine discontinuation, such patients subjectively report improved symptoms. However, although low vitamin B6 levels can be seen in patients suffering from peripheral neuropathy of various etiologies, there is no firm evidence that low B6 levels have a direct causal relationship with PN. Many studies suggest subjective improvement of neuropathy symptoms in patients suffering from PN of various etiologies after receiving B6 supplementation; however, no data about B6 administration as a monotherapy exist, only as part of a combination treatment, usually with other vitamins. Therefore, the potential therapeutic role of B6 cannot be confirmed to date. Supplementation with vitamin B6, even as part of a nutritional multivitamin supplement, has not been proven harmful at permitted daily doses in patients who already suffer from PN. Conclusion: Current scientific evidence supports a neurotoxic role of B6 at high levels. Although some studies suggest that low B6 is also a potential risk factor, further studies in this area are needed.
The Reciprocal Relationship between Climate and Environmental Changes and Food Systems and Its Impact on Food/Nutrition Security and Health
Authors: Bremer Andrew A. , Raiten Daniel J.
Published: June 2023

Changes in our climate and physical environments are having profound effects on all aspects of human existence, and the ability to develop sustainable and resilient food systems is critical not just to the environment but to all aspects of human health. The Pediatric Growth and Nutrition Branch (PGNB) of the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the US National Institutes of Health has adopted a new paradigm to undergird the study of nutrition that recognizes the complex and reciprocal nature of the relationships between nutrition and health outcomes. This conceptual framework, termed the “nutritional ecology,” views humans as complex biological systems interacting with both their internal and external environments. Herein, we focus on: (i) the reciprocal relationship between climate and environmental changes and food systems and their impact on food/nutrition security and health; and (ii) how PGNB is utilizing the “nutritional ecology” framework to support science addressing the interactions among health, nutrition, food systems, climate, and the environment.
Risk Factors for Hepatitis E Virus Infection and Eating Habits in Kidney Transplant Recipients
Authors: Wu Eva , Koch Nadine , Bachmann Friederike , Schulz Marten , Seelow Evelyn , Weber Ulrike , Waiser Johannes , Halleck Fabian , Faber Mirko , Bock Claus-Thomas , Eckardt Kai-Uwe , Budde Klemens , Hofmann Jörg , Nickel Peter , Choi Mira
Published: June 2023

There is a significant risk for ongoing and treatment-resistant courses of hepatitis E virus (HEV) infection in patients after solid organ transplantation. The aim of this study was to identify risk factors for the development of hepatitis E, including the dietary habits of patients. We conducted a retrospective single-center study with 59 adult kidney and combined kidney transplant recipients who were diagnosed with HEV infection between 2013 and 2020. The outcomes of HEV infections were analyzed during a median follow-up of 4.3 years. Patients were compared with a control cohort of 251 transplant patients with elevated liver enzymes but without evidence of an HEV infection. Patients’ alimentary exposures during the time before disease onset or diagnosis were assessed. Previous intense immunosuppression, especially treatment with high-dose steroids and rituximab, was a significant risk factor to acquire hepatitis E after solid organ transplantation. Only 11 out of 59 (18.6%) patients reached remission without further ribavirin (RBV) treatment. A total of 48 patients were treated with RBV, of which 19 patients (39.6%) had either viral rebounds after the end of treatment or did not reach viral clearance at all. Higher age (>60 years) and a BMI ≤ 20 kg/m2 were risk factors for RBV treatment failure. Deterioration in kidney function with a drop in eGFR (p = 0.046) and a rise in proteinuria was more common in patients with persistent hepatitis E viremia. HEV infection was associated with the consumption of undercooked pork or pork products prior to infection. Patients also reported processing raw meat with bare hands at home more frequently than the controls. Overall, we showed that the intensity of immunosuppression, higher age, a low BMI and the consumption of undercooked pork meat correlated with the development of hepatitis E.
Characteristics of Extracellular Vesicles from a High-Grade Serous Ovarian Cancer Cell Line Derived from a Platinum-Resistant Patient as a Potential Tool for Aiding the Prediction of Responses to Chemotherapy
Authors: Černe Katarina , Kelhar Nuša , Resnik Nataša , Herzog Maruša , Vodnik Lana , Veranič Peter , Kobal Borut
Published: June 2023

Platinum-resistant high-grade serous ovarian cancer (HGSOC) is invariably a fatal disease. A central goal of ovarian cancer research is therefore to develop new strategies to overcome platinum resistance. Treatment is thus moving towards personalized therapy. However, validated molecular biomarkers that predict patients’ risk of developing platinum resistance are still lacking. Extracellular vesicles (EVs) are promising candidate biomarkers. EpCAM-specific EVs are largely unexplored biomarkers for predicting chemoresistance. Using transmission electron microscopy, nanoparticle tracking analysis and flow cytometry, we compared the characteristics of EVs released from a cell line derived from a clinically confirmed cisplatin-resistant patient (OAW28) and EVs released from two cell lines from tumors sensitive to platinum-based chemotherapy (PEO1 and OAW42). We demonstrated that EVs released from the HGSOC cell line of chemoresistant patients exhibited greater size heterogeneity, a larger proportion of medium/large (>200 nm) Evs and a higher number of released EpCAM-positive EVs of different sizes, although the expression of EpCAM was predominant in EVs larger than 400 nm. We also found a strong positive correlation between the concentration of EpCAM-positive EVs and the expression of cellular EpCAM. These results may contribute to the prediction of platinum resistance in the future, although they should first be validated in clinical samples.
A Review on Natural Antioxidants for Their Role in the Treatment of Parkinson’s Disease
Authors: Mittal Pooja , Dhankhar Sanchit , Chauhan Samrat , Garg Nitika , Bhattacharya Tanima , Ali Maksood , Chaudhary Anis Ahmad , Rudayni Hassan Ahmad , Al-Zharani Mohammed , Ahmad Wasim , Khan Salah Ud-Din , Singh Thakur Gurjeet , Mujwar Somdutt
Published: June 2023

The neurodegenerative condition known as Parkinson’s disease (PD) is brought on by the depletion of dopaminergic neurons in the basal ganglia, which is the brain region that controls body movement. PD occurs due to many factors, from which one of the acknowledged effects of oxidative stress is pathogenic pathways that play a role in the development of Parkinson’s disease. Antioxidants, including flavonoids, vitamins E and C, and polyphenolic substances, help to reduce the oxidative stress brought on by free radicals. Consequently, this lowers the risk of neurodegenerative disorders in the long term. Although there is currently no cure for neurodegenerative illnesses, these conditions can be controlled. The treatment of this disease lessens its symptoms, which helps to preserve the patient’s quality of life. Therefore, the use of naturally occurring antioxidants, such as polyphenols, which may be obtained through food or nutritional supplements and have a variety of positive effects, has emerged as an appealing alternative management strategy. This article will examine the extent of knowledge about antioxidants in the treatment of neurodegenerative illnesses, as well as future directions for research. Additionally, an evaluation of the value of antioxidants as neuroprotective agents will be provided.
The Chemometric Evaluation of the Factors Influencing Cloud Point Extraction for Fluoroquinolones
Authors: Michałowska Aleksandra , Kupczyk Olga , Czyrski Andrzej
Published: June 2023

This study aimed to analyze the factors that impact the cloud point extraction of ciprofloxacin, levofloxacin, and moxifloxacin. The following independent variables were analyzed: Triton X-114 concentration, NaCl concentration, pH, and incubation temperature. The dependent variable studied was recovery. A central composite design model was used. The applied quantitation method was HPLC. The method was validated for linearity, precision, and accuracy. The results underwent ANOVA® analysis. The polynomial equations were generated for each analyte. The response surface methodology graphs visualized them. The analysis showed that the factor most affecting the recovery of levofloxacin is the concentration of Triton X-114, while the recovery of ciprofloxacin and moxifloxacin is most affected by pH value. However, the concentration of Triton X-114 also plays an important role. The optimization resulted in the following recoveries: for ciprofloxacin, 60%; for levofloxacin, 75%; and for moxifloxacin, 84%, which are identical to those estimated with regression equations—59%, 74% and 81% for ciprofloxacin, levofloxacin, and moxifloxacin, respectively. The research confirms the validity of using the model to analyze factors affecting the recovery of the analyzed compounds. The model allows for a thorough analysis of variables and their optimization.
Fluorescent and Magnetic Radical Dendrimers as Potential Bimodal Imaging Probes
Authors: Zhang Songbai , Lloveras Vega , Wu Yufei , Tolosa Juan , García-Martínez Joaquín C. , Vidal-Gancedo José
Published: June 2023

Dual or multimodal imaging probes have emerged as powerful tools that improve detection sensitivity and accuracy in disease diagnosis by imaging techniques. Two imaging techniques that are complementary and do not use ionizing radiation are magnetic resonance imaging (MRI) and optical fluorescence imaging (OFI). Herein, we prepared metal-free organic species based on dendrimers with magnetic and fluorescent properties as proof-of-concept of bimodal probes for potential MRI and OFI applications. We used oligo(styryl)benzene (OSB) dendrimers core that are fluorescent on their own, and TEMPO organic radicals anchored on their surfaces, as the magnetic component. In this way, we synthesized six radical dendrimers and characterized them by FT-IR, 1H NMR, UV-Vis, MALDI-TOF, SEC, EPR, fluorimetry, and in vitro MRI. Importantly, it was demonstrated that the new dendrimers present two properties: on one hand, they are paramagnetic and show the ability to generate contrast by MRI in vitro, and, on the other hand, they also show fluoresce emission. This is a remarkable result since it is one of the very few cases of macromolecules with bimodal magnetic and fluorescent properties using organic radicals as the magnetic probe.
Drug Delivery in Photodynamic Therapy
Authors: Moret Francesca , Varchi Greta
Published: June 2023

Photodynamic therapy (PDT) has gained prominence as a non-invasive and selective treatment option for solid tumors and non-oncological diseases [...]
Properties of Scattering Fields from Gaussian Beam Incident on Rough Cylinders
Authors: Ye Shubing , Feng Guobin , Feng Zhejun , Wang Zhenbao , Wang Dahui , Cao Changqing
Published: June 2023

At present, most researchers focus on plane wave incident on targets, but in practical applications, most of the beams are Gaussian beam. We study the scattering fields of Gaussian beam incident on rough cylinders. Coherent and incoherent scattering coefficients are obtained based on the angular spectrum expansion and physical optics approximation, and the effects of cylinder roughness, beam radius, cylinder radius and angle of incidence on scattering coefficients are analyzed. The results show that, for a constant wavelength, when the root mean square height is greater than or equal to 1/5 of the wavelength, the coherent scattering coefficient curve undergoes a change in its distribution, with the peak transforming into a trough. Furthermore, when the root mean square height is greater than or equal to 1/3 of the wavelength, the incoherent scattering coefficient experiences a decline as the root mean square height increases. The correlation length only affects the incoherent scattering coefficient. Both the coherent and incoherent scattering coefficients decrease with the increase in the incident angle. Finally, when the roughness and incident angle are constant, with the increase in the ratio of the cylinder radius to the beam waist radius, the scattered light field is more concentrated. Our results provide the theoretical basis for the measurement of the cylindrical scattering field.
Phase Regeneration of QPSK Signals Based on Kerr Soliton Combs
Authors: Han Xinjie , Geng Yong , Ke Haocheng , Qiu Kun
Published: June 2023

We demonstrate a phase-sensitive and amplification-based all-optical phase regenerator by utilizing on-chip Kerr soliton combs. In the experiment, we demonstrate the direct generation of a Kerr soliton comb in a silicon nitride micro-ring at the receiver side of optical communication systems by applying the transmitted signal as a pump light. The mutual coherence between the signal and the regenerated Kerr comb is excellent, and the all-optical phase regeneration of a 20 GBaud/s QPSK signal is achieved. In contrast to the traditional scheme, our solution shows better SWaP (size, weight, and power) factors. Our study will enhance the relay and reception performance of all-optical communication systems.
Multiple Fano Resonances in a Metal–Insulator–Metal Waveguide for Nano-Sensing of Multiple Biological Parameters and Tunable Slow Light
Authors: Zhang Ruiqi , Tian He , Liu Yang , Cui Shihang
Published: June 2023

A surface plasmonic waveguide made of metal–insulator–metal (MIM) capable of generating triple Fano resonances is proposed and numerically investigated for multi-biological parameter sensing as well as tunable slow light. The waveguide is made up of a bus waveguide with a silver baffle, a square split-ring cavity with a square center (SSRCSC), and a circular ring cavity with a square center (CRCSC). Based on the triple Fano resonances, human blood temperature and plasma concentration are measured simultaneously at different locations in the waveguide, and the maximum sensitivities were 0.25 nm/°C and 0.2 nm·L/g, respectively. Furthermore, the two biological parameters can be used to achieve tunable slow light, and it was found that the group delay responses to human blood temperature and plasma concentration all conformed to cubic functions. The MIM waveguide may have great applications in future nano-sensing of multiple biological parameters and information processing of optical chips or bio-optical chips.
Fluorescent Microscopy of Hot Spots Induced by Laser Heating of Iron Oxide Nanoparticles
Authors: Ryabova Anastasia , Pominova Daria , Markova Inessa , Nikitin Aleksey , Ostroverkhov Petr , Lasareva Polina , Semkina Alevtina , Plotnikova Ekaterina , Morozova Natalia , Romanishkin Igor , Linkov Kirill , Abakumov Maksim , Pankratov Andrey , Steiner Rudolf , Loschenov Victor
Published: June 2023

Determination of the iron oxide nanoparticles (IONPs) local temperature during laser heating is important in the aspect of laser phototherapy. We have carried out theoretical modeling of IONPs local electromagnetic (EM) field enhancement and heating under the laser action near individual IONPs and ensembles of IONPs with different sizes, shapes and chemical phases. For experimental determination of IONPs temperature, we used fluorescence thermometry with rhodamine B (RhB) based on its lifetime. Depending on the IONPs shape and their location in space, a significant change in the spatial distribution of the EM field near the IONPs surface is observed. The local heating of IONPs in an ensemble reaches sufficiently high values; the relative change is about 35 °C for Fe2O3 NPs. Nevertheless, all the studied IONPs water colloids showed heating by no more than 10 °C. The heating temperature of the ensemble depends on the thermal conductivity of the medium, on which the heat dissipation depends. During laser scanning of a cell culture incubated with different types of IONPs, the temperature increase, estimated from the shortening of the RhB fluorescence lifetime, reaches more than 100 °C. Such “hot spots” within lysosomes, where IONPs predominantly reside, lead to severe cellular stress and can be used for cell therapy.
New Reports on the Portuguese Endemic Species, Santolina impressa: Secretory Structures, Essential Oil Composition and Antiviral Activity
Authors: Rodrigues Ana Margarida , Mendes Ana Rita , Caeiro Maria Filomena , Figueiredo Ana Cristina , Ascensão Lia
Published: June 2023

Santolina impressa is an aromatic Asteraceae species endemic to Portugal, traditionally used for its anti-inflammatory properties. The aim of this study was to characterize S. impressa secretory structures, analyze the essential oil (EO) from the aerial organs, and evaluate its antiviral activity against herpes simplex viruses HSV-1 and HSV-2. Secretory structures were investigated by light and scanning microscopy, and the secretion was histochemically characterized. The EO from the aerial organs in full blooming was analyzed by gas chromatography with flame ionization detection and gas chromatography–mass spectrometry. Antiviral assays were performed by direct contact with viral suspensions (virucidal effect), and in infected Vero E6 cells, at different time periods during the viral replication cycle. Two types of secretory structures were described, biseriate glandular trichomes and secretory ducts, producing an oleoresin and a resin rich in flavonoids, respectively. Fifty compounds were identified in S. impressa EO, accounting for 87% of the total constituents. Monoterpenes constituted the main EO fraction (82%), with β-pinene (13%) and β-phellandrene (10%) being their major components. The EO interacted with HSV-1 and HSV-2 in a dose-dependent manner, thereby inactivating both viral infections. The EO did not evidence a virucidal effect but inhibited the HSV-1 and HSV-2 infection in Vero cells in a dose-dependent manner. However, further studies are needed to investigate the mode of action in the replication cycle.
Development of Fatigue Life Model for Rubber Materials Based on Fracture Mechanics
Authors: Qiu Xingwen , Yin Haishan , Xing Qicheng , Jin Qi
Published: June 2023

In this paper, the research on the fatigue damage mechanism of tire rubber materials is the core, from designing fatigue experimental methods and building a visual fatigue analysis and testing platform with variable temperature to fatigue experimental research and theoretical modeling. Finally, the fatigue life of tire rubber materials is accurately predicted by using numerical simulation technology, forming a relatively complete set of rubber fatigue evaluation means. The main research is as follows: (1) Mullins effect experiment and tensile speed experiment are carried out to explore the standard of the static tensile test, and the tensile speed of 50 mm/min is determined as the speed standard of plane tensile, and the appearance of 1 mm visible crack is regarded as the standard of fatigue failure. (2) The crack propagation experiments were carried out on rubber specimens, and the crack propagation equations under different conditions were constructed, and the relationship between temperature and tearing energy was found out from the perspective of functional relations and images, and the analytical relationship between fatigue life and temperature and tearing energy was established. Thomas model and thermo-mechanical coupling model were used to predict the life of plane tensile specimens at 50 °C, and the predicted results were 8.315 × 105 and 6.588 × 105, respectively, and the experimental results were 6.42 × 105, with errors of 29.5% and 2.6%, thus verifying the accuracy of thermo-mechanical coupling model.
Facile Synthesis Hyper-Crosslinked PdFe Bimetallic Polymer as Highly Active Catalyst for Ullmann Coupling Reaction of Chlorobenzene
Authors: Tang Cheng , Yang Wenwen , Zou Zhijuan , Liao Fang , Zeng Chunmei , Song Kunpeng
Published: June 2023

The synthesis of efficient and sustainable heterogeneous Pd-based catalysts has been an active field of research due to their crucial role in carbon–carbon coupling reactions. In this study, we developed a facile and eco-friendly in situ assembly technique to produce a PdFe bimetallic hyper-crosslinked polymer (HCP@Pd/Fe) to use as a highly active and durable catalyst in the Ullmann reaction. The HCP@Pd/Fe catalyst exhibits a hierarchical pore structure, high specific surface area, and uniform distribution of active sites, which promote catalytic activity and stability. Under mild conditions, the HCP@Pd/Fe catalyst is capable of efficiently catalyzing the Ullmann reaction of aryl chlorides in aqueous media. The exceptional catalytic performance of HCP@Pd/Fe is attributed to its robust absorption capability, high dispersion, and strong interaction between Fe and Pd, as confirmed by various material characterizations and control experiments. Furthermore, the coated structure of a hyper-crosslinked polymer enables easy recycling and reuse of the catalyst for at least 10 cycles without any significant loss of activity.
Numerical Investigations of Heat Transfer and Fluid Flow Characteristics in Microchannels with Bionic Fish-Shaped Ribs
Authors: Gao Quanyue , Zou Haibo , Li Juan
Published: June 2023

Microchannel cooling technology is an effective method to solve local thermal stacking. In this paper, four innovative microchannels with bionic fish-shaped rib arrangements (CM-O, CM-R, CM-H, and CM-G) are designed by imitating geese and fish clusters. The heat transfer and flow characteristics of the microchannels are simulated numerically at different Reynold’s numbers (Re = 200 − 1600). The liquid water temperature and flow field in the four innovative microchannels with bionic ribs are analyzed. The results show that the ribs’ arrangement has an influence on the thermal performance of microchannels. Compared to the smooth microchannel (CM), the of the Nu microchannels with the bionic fish-shaped ribs increases by 33.00–53.26% while the fave increases by 28.63–34.93% at Re = 1200. The vortices around the ribs are clearly observed which improves the temperature gradient. The performance evaluation criterion (PEC) of CM-H is higher than that of the others. This indicates that the rib arrangement of CM-H is superior for heat dissipation application.