bluuebunny/crossref_metadata_2025 huggingface dataset:
The heterogeneity of the Mexican lithospheric mantle: Clues from noble gas and CO2 isotopes in fluid inclusions
Authors: Sandoval-Velasquez Andres , Rizzo Andrea Luca , Aiuppa Alessandro , Straub Susanne M. , Gomez-Tuena Arturo , Espinasa-Perena Ramon
Published: September 2022

The abundance of mantle-derived rocks and lavas, in combination with its tectonic evolution, render Mexico a perfect laboratory to investigate the chemical and the isotopic heterogeneity of the lithospheric mantle. New data on the composition of noble gases and CO2in Mexican mantle xenoliths and lavas is reported. Our samples consist of six ultramafic nodules from the Durango Volcanic Field (DVF) and the San Quintin Volcanic Field (SQVF), monogenetic complexes belonging to the Mexican Basin and Range province; and four lavas from the Sierra Chichinautzin (SCN), a Quaternary monogenetic volcanic field located in the Mexican volcanic arc. Ne and Ar isotopes in fluid inclusions reveal mixing between atmospheric and MORB-like fluids (e.g.,40Ar/36Ar < 1,200). DVF and SQVF nodules record low40Ar/36Ar and4He/20Ne that confirm the existence of recycled atmospheric-derived noble gases in the local mantle. The averages of the Rc/Ra ratios (3He/4He corrected for atmospheric contamination) measured in Mexican localities are within the MORB-like range: DVF= 8.39 ± 0.24 Ra, SQVF = 7.43 ± 0.19 Ra and SCN lavas = 7.15 ± 0.33 Ra (1σ). With the aim of assessing the isotopic variability of the Mexican lithospheric mantle, the above results were compared with similar data previously obtained from ultramafic nodules found in the Ventura Espiritu Santo Volcanic Field (VESVF), another Quaternary monogenetic volcanic complex belonging the Basin and Range. The higher3He/4He ratios in DVF relative to those reported for the VESVF and the SQVF are explained as reflecting different ages of mantle refertilization, triggered by the retreating of the Farallon slab (∼40 Ma ago) and associated delamination slab processes. We propose that the DVF mantle was refertilized more recently (<10 Ma ago) than the mantle beneath the SQVF and VESVF (∼40–20 Ma ago). On the other hand, He-Ne-Ar compositions of SCN olivines share similarities with VESVF xenoliths, suggesting a relatively homogeneous lithospheric mantle in central Mexico. Finally, DVF and the SCN samples exhibit δ13C values within the MORB range (comparable to other values previously reported in fluid inclusions and fumaroles from Popocatépetl, Colima—Ceboruco volcanoes). While we explain the MORB-like carbon signatures of the DVF samples as the result of the above-mentioned refertilization process, the SCN signatures likely reflect either (i) trapping of isotopically fractionated CO2derived from magmatic degassing or (ii) a mantle source unaffected by subduction-related crustal carbon recycling.
Identification of the early cretaceous granitic pluton and tectonic implications in the middle gangdese belt, southern tibet
Authors: Wang Qingling , Meng Yuanku , Wei Youqing , Jiang Li , Wang Zhenzhen , Mao Guangzhou
Published: September 2022

The Gangdese magmatic belt of southern Tibet is an ideal place to study Neo-Tethyan subduction, continental crustal growth and reworking. However, there are still controversies with regard to the evolution of the Neo-Tethys Ocean, the magma source and the detailed diagenetic processes of igneous rocks in the Gangdese belt. The Early Cretaceous magmatic exposures are sporadic in the Gangdese magmatic belt. Thus the finding of the new exposure is key to understanding the scenarios of the Neo-Tethys Ocean and geological background of southern Tibet during the Early Cretaceous. In this contribution, we undertook systematic geochronology, whole-rock geochemistry and zircon Lu-Hf isotopic studies on a newly identified granitic pluton in the middle Gangdese belt (Quesang area), southern Tibet. The results show that zircon U-Pb dating of three representative samples yielded a weighted age of 120 ± 1.4 Ma, 117.3 ± 2.5 Ma and 114.0 ± 1.3 Ma, respectively, which indicate the emplacement and crystallization age belonging to the Aptian stage of the Early Cretaceous in response to the northward subduction of the Neo-Tethyan oceanic lithosphere beneath the Lhasa terrane. In situ zircon Lu-Hf isotopic analyses indicate that ƐHf(t) values of the studied granitic pluton are predominantly positive ranging from 7.2 to 11.4, and one zircon shows negative ƐHf(t) value (−6.26), implying that a small amount of ancient continental crustal materials might have participated in the magma evolution. Geochemically, the granite samples are enriched in large ion lithophile elements (LILE) and light rare earth elements (LREE), but depleted in heavy rare earth elements (HREE), indicating arc-type geochemical characteristics or subduction-related tectonic setting. In addition, combined with mineral assemblages, zircon Lu-Hf isotopic features and low molar Al2O3/(CaO+Na2O+K2O) (A/CNK) ratios of 0.91–1.10, the studied samples show a close affinity with I-type granites. Moreover, zircon oxygen fugacity results show that Ce4+/Ce3+ values range from 185 to 12, with a mean value of 78, indicating a low oxygen fugacity setting similar to the Chile ore-barren granitic plutons. In combination with published data, we argue that the Gangdese magmatic belt may have developed continuous magmatism (145–105 Ma), and the notion of magmatic lull might deserve more consideration during the Early Cretaceous. In this study, the Early Cretaceous granitic pluton might be the result of the northward subduction of Neo-Tethys oceanic lithosphere beneath the Lhasa terrane at a normal angle.
Study on the stability of high and steep slopes under deep bench blasting vibration in open-pit mines
Authors: Su Hengyu , Ma Shu
Published: September 2022

In order to study the stability of the high and steep slope of an open-pit mine under deep bench blasting vibration, a mine in Inner Mongolia is taken as the engineering background, and the mechanical parameters of rock samples were determined based on uniaxial and triaxial instruments. The stability of the high and steep slope of the open-pit mine under static and dynamic loads was analyzed by using field vibration monitoring and numerical simulation methods. The results show that the vibration range of the vibration wave is -1.25–1.25 cm/s, and the vibration wave shows a gradual attenuation trend. The Sadovsky regression equation was used to analyze and fit the monitoring data and the corresponding regression equations in each direction were obtained. Under static action, the safety factor of the high and steep slope is 1.20, and the displacement of the sliding zone passing through the slope is small, so the slope stability is good. Under the action of dynamic blasting load, the overall displacement of the slope is small, and the change of displacement decreases with the decrease of the vibration wave.
Geodynamic seawater-sediment porewater evolution of the east central Atlantic Paleogene ocean margin revealed by U-Pb dating of sedimentary phosphates
Authors: Aubineau Jérémie , Parat Fleurice , Chi Fru Ernest , El Bamiki Radouan , Mauguin Olivia , Baron Fabien , Poujol Marc , Séranne Michel
Published: September 2022

Emerging evidence suggests that U-Pb and Lu-Hf ages of sedimentary apatite group minerals are often younger than their biostratigraphic ages. However, U-Pb dating of exquisitely preserved carbonate fluorapatite (CFA) is rare. The Upper Cretaceous/Paleogene marine sedimentary rocks of the Moroccan High Atlas host phosphate-rich sediments bracketed by calcareous nannofossil Zones (NP4-NP9) of late Danian to Thanetian age. Here, we use a laser-ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to decipher whether CFA minerals are suitable for U-Pb chronostratigraphy and whether they can reveal the sedimentary and seawater history from which they formed. U-Pb dating of the CFA grains yields ages of 42.9 ± 1.3 Ma (MSWD = 2.3) and 35.7 ± 2.8 Ma (MSWD = 1.3) from three distinct phosphate-rich beds, being >15 million years younger than the expected biostratigraphic age. Combined scanning electron microscopy, X-ray diffraction, and infrared spectroscopy analyses, associate the Mg-rich clay minerals sepiolite and palygorskite, with micro-CFA crystals, while LA-ICP-MS trace element, rare earth element, and yttrium content for primary CFA grains, collectively point to long-term early diagenetic adsorption from oxygenated seawater-dominated porewater fluids. Authigenic clay minerals display a seawater-like pattern, with negligible U concentrations suggesting limited clay mineral influence on U-Pb dating of the CFA crystals. Considering the absence of extensive post-depositional alteration, we propose that because of their large surface area, the µm-sized CFA crystallites facilitated real-time surface adsorption and desorption of elements and diffusion processes. These conditions generated long-term open system connection of sediments with overlying seawater, enabling continuous U-Pb exchange for 15–25 Myr after phosphate precipitation. The data suggest that system closure was potentially associated with sediment lithification and the Atlas orogeny, pointing to stable oxygenation of shallow marine waters along the eastern passive margin of the central Atlantic Ocean in the Paleogene.
Where and why to conserve grasslands socio-ecosystems? A spatially explicit participative approach
Authors: Staiano Luciana , Gallego Federico , Altesor Alice , Paruelo José M.
Published: September 2022

Grasslands of southern South America are being replaced by annual crops and forest plantations. The environmental and social consequences of this expansion generate the need for its regulation. If a conservation policy were established, it would be critical to define which areas would have priority for conservation. Multi-criteria analysis techniques are useful tools in territorial planning processes since they allow incorporating diverse and even opposing opinions and objectives. We present a methodological approach to define the Grasslands’ Conservation Value (GCV) from a spatially explicit territorial diagnosis, based on multiple criteria and incorporating explicitly and quantitatively the valuations and opinions of stakeholders. The study was developed as part of the strategy of a public inter-institutional entity to contribute in defining grasslands conservation policies. The methodological approach included workshops in which the definitions of the conservation criteria and their weighting were agreed upon. Definitions were based on a multidimensional technical characterization of the territory through indicators, for which the information used was compiled, analyzed, shared, and synthesized. Based on multi-criteria analysis, each of 12 stakeholders’ groups representatives established the individual weighting of the criteria for determining the GCV and then, established a consensus weighting. The GCV was mapped by integrating territorial diagnosis of these criteria with the weightings carried out by the stakeholders. The degree of agreement among stakeholders in the differential valuation of the ecological criteria was high for 8 of the 12 stakeholders (Pearson’s correlation coefficients >0.92), showing a high agreement between their opinions and those resulting from the group consensus. In all cases, the agreement about the spatial variation of conservation value was higher than on the criteria weights (Pearson’s correlation coefficients ≥0.92 for 10 stakeholders). Furthermore, the sites with lower values in the consensus map corresponded mostly to those sites with lower agreement among stakeholders. The proposed methodology allowed the incorporation of different perceptions not only in the definition of conservation criteria but also in their prioritization, in a transparent and auditable process. This could contribute to the implementation of future regulations that restrict the replacement of grasslands, increasing the legitimacy of territorial planning processes.
A novel lactate metabolism-related signature predicts prognosis and tumor immune microenvironment of breast cancer
Authors: Zhang Zhihao , Fang Tian , Lv Yonggang
Published: September 2022

Background: Lactate, an intermediate product of glycolysis, has become an essential regulator of tumor maintenance, development, and metastasis. Lactate can drive tumors by changing the microenvironment of tumor cells. Because of lactate’s important role in cancer, we aim to find a novel prognostic signature based on lactate metabolism-related genes (LMRGs) of breast cancer (BC).

Methods: RNA-sequencing data and clinical information of BC were enrolled from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. We obtained LMRGs from the Molecular Signature Database v7.4 and articles, and then we compared candidate genes with TCGA data to get differential genes. Univariate analysis and most minor absolute shrinkage and selector operator (LASSO) Cox regression were employed to filter prognostic genes. A novel lactate metabolism-related risk signature was constructed using a multivariate Cox regression analysis. The signature was validated by time-dependent ROC curve analyses and Kaplan–Meier analyses in TCGA and GEO cohorts. Then, we further investigated in depth the function of the model’s immune microenvironment.

Results: We constructed a 3-LMRG-based risk signature. Kaplan–Meier curves confirmed that high-risk score subgroups had a worse prognosis in TCGA and GEO cohorts. Then a nomogram to predict the probability of survival for BC was constructed. We also performed Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway function analysis. The function analysis showed that the lactate metabolism-related signature was significantly related to immune response. A significant correlation was observed between prognostic LMRGs and tumor mutation burden, checkpoints, and immune cell infiltration. An mRNA–miRNA network was built to identify an miR-203a-3p/LDHD/LYRM7 regulatory axis in BC.

Conclusion: In conclusion, we constructed a novel 3-LMRG signature and nomogram that can be used to predict the prognosis of BC patients. In addition, the signature is closely related to the immune microenvironment, which may provide new insight into future anticancer therapies.
Short birth interval and its associated factors among multiparous women in Mieso agro-pastoralist district, Eastern Ethiopia: A community-based cross-sectional study
Authors: Wakeyo Musa Mohammed , Kebira Jemal Yusuf , Assefa Nega , Dheresa Merga
Published: September 2022

Background

Recently, the concern with birth interval has acquired importance in public health and family planning because of its implication for fertility, maternal, and child health. A short birth interval is associated with adverse perinatal, maternal, and infant outcomes. Moreover, too short birth interval lead to high fertility, which in turn contributes to accelerated population growth and undermines development efforts. This study aimed to investigate the prevalence of short birth interval and its associated factors among multiparous women in the Mieso agro-pastoralist district, Oromia region, Eastern Ethiopia.

Methods

A community-based cross-sectional study was conducted from 1 to 30 March 2020. The multistage sampling technique was used to select 490 multiparous women. Data were collected by face-to-face interviewer-administered structured questionnaires. Bivariate and multivariable logistic regression analyses were executed. Model fitness and multicollinearity were checked. Statistically significant associations of outcome and independent variables were declared at a P-value of < 0.05.

Results

The prevalence of short birth interval was 56% (95% CI: 51.4–60.5) in the study area. Being married under 18 years (AOR = 3.78, 95% CI: 1.97–7.25), having formal education (AOR = 0.23, 95% CI: 0.11–0.47), having a husband with formal education (AOR = 0.46, 95% CI: 0.22–0.99), having awareness about optimum birth interval (AOR = 0.47, 95% CI: 0.24–0.91), having female index child (AOR = 1.78, 95% CI: 1.07–3.84), death of the index child (AOR = 0.34, 95% CI: 0.12–0.92), breastfeeding of the index child <24 months (AOR = 2.6, 95% CI: 1.53–4.41), use of modern contraceptive (AOR = 2.09, 95% CI: 1.12–3.89), and decision-making by a husband alone when to have a child (AOR = 3.86, 95% CI: 2.06–7.21) were significantly associated with short birth interval at a P-value <0.05.

Conclusion

The overall prevalence of short birth interval among the study participants was high, as more than half of the women had practiced short birth interval, indicating that the majority of the mother and children in the study area are still at high risk of mortality and morbidity associated with short birth interval. Thus, the current findings suggest that interventions that involve the provision of contraceptives and information on its benefit at points need to be adopted to reach the national and global target of maternal and child mortality reduction attributed to short birth interval.
Association between IgG responses against the nucleocapsid proteins of alphacoronaviruses and COVID-19 severity
Authors: Nückel Julius , Planatscher Elisa , Mohr Anne Wiebe , Deichl Karolin , Mijočević Hrvoje , Feuerherd Martin , Wolff Lisa , Erber Johanna , Schneider Jochen , Quante Michael , Winter Christoph , Ruland Jürgen , Hapfelmeier Alexander , Hammerschmidt Wolfgang , Moosmann Andreas , Protzer Ulrike , Behrends Uta , Mautner Josef
Published: September 2022

Understanding immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to contain the COVID-19 pandemic. Using a multiplex approach, serum IgG responses against the whole SARS-CoV-2 proteome and the nucleocapsid proteins of endemic human coronaviruses (HCoVs) were measured in SARS-CoV-2-infected donors and healthy controls. COVID-19 severity strongly correlated with IgG responses against the nucleocapsid (N) of SARS-CoV-2 and possibly with the number of viral antigens targeted. Furthermore, a strong correlation between COVID-19 severity and serum responses against N of endemic alpha- but not betacoronaviruses was detected. This correlation was neither caused by cross-reactivity of antibodies, nor by a general boosting effect of SARS-CoV-2 infection on pre-existing humoral immunity. These findings raise the prospect of a potential disease progression marker for COVID-19 severity that allows for early stratification of infected individuals.
Whole course of treatment of autoimmune progesterone dermatitis that had spontaneously resolved during pregnancy: A case report and review of the literature
Authors: Huang Yepei , Ye Sha , Bao Xiaoyan , Yang Ru , Huang Jian
Published: September 2022

Anaphylaxis due to autoimmune progesterone dermatitis is a rare but severe allergic disease in women. The clinical manifestations of APD are diverse, and a proper understanding of the disease can help even diagnose and treat it. A case ofautoimmune progesterone dermatitisrelated in our department is reported as follows. She developed a rash with severe pruritus that was highly consistent with her menstrual cycle without any trigger 10 years ago. Laboratory tests were unremarkable. But all the symptoms disappeared during her pregnancy and resurfaced after the miscarriage. Two years ago, after a positive progesterone intradermal test confirmed the diagnosis ofPH, she was given mifepristone, contraceptives(OCPs), and skin embedding treatment, and her symptoms improved.
Monoclonal anti-endoglin antibody TRC105 (carotuximab) prevents hypercholesterolemia and hyperglycemia-induced endothelial dysfunction in human aortic endothelial cells
Authors: Tripska Katarina , Igreja Sá Ivone Cristina , Vasinova Martina , Vicen Matej , Havelek Radim , Eissazadeh Samira , Svobodova Zuzana , Vitverova Barbora , Theuer Charles , Bernabeu Carmelo , Nachtigal Petr
Published: September 2022

Endoglin (Eng) is a co-receptor of the transforming growth factor β superfamily playing an important role in endothelial dysfunction. TRC105 (carotuximab) is a monoclonal antibody that blocks Eng and its downstream Smad signaling pathway. Here we have investigated for the first time the effects of TRC105 treatment on the development of endothelial dysfunction induced by 7-ketocholesterol (7K) or high glucose (HG), focusing on Eng expression, signaling, and function. In the hypercholesterolemia study, human aortic endothelial cells (HAoECs) were treated with TRC105 (300 μg/ml) for 1 h, followed by the addition of 7K (10 μg/ml) for another 12 h. In the hyperglycemia study, HAoECs were exposed to HG (45 mM) for 60 h, followed by the addition of TRC105 for another 12 h, and cells treated with 5mM glucose and 40 mM mannitol served as control. Protein levels, adhesion, and transmigration of monocytes were assessed by flow cytometry, mRNA expression was measured by qRT-PCR. 7K and HG treatment increased protein levels of NF-κB and Eng and adhesion and transmigration of monocytes through HAoECs monolayer. TRC105 pretreatment reduced the 7K- or HG-induced Eng protein levels and pSmad1/5 and pSmad2/3 signaling. Despite increased protein levels of P-selectin and VCAM-1, TRC105 mediated blockage of Eng prevented 7K- and HG-induced adhesion and transmigration of monocytes through endothelial monolayers. These results suggest that TRC105-mediated Eng blockage can counteract the hypercholesterolemia- and hyperglycemia-induced endothelial dysfunction in HAoECs, suggesting that Eng might be a potential therapeutic target in disorders associated with elevated cholesterol and glucose levels.
Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection
Authors: Xu Wen , Du Juan , Wei Ting-Ting , Chen Lin-Yi , Yang Xin-Xin , Bo Tu , Liu Han-Yu , Xie Ming-Zhu , Zhao Tian-Shuo , Yang Jun-Lian , Cui Fuqiang , Chen Wei-Wei , Lu Qing-Bin
Published: September 2022

Objectives

The changes in metabolism by human adenovirus (HAdV) infection was unclear. The potential mechanism of HAdV-7 causing acute respiratory tract infection was explored.

Methods

Totally 35 patients with HAdV-7 infection, 32 asymptomatic cases with HAdV-7 and 14 healthy controls were enrolled from an outbreak of HAdV-7 in the army. The serum samples were analyzed by untargeted and targeted metabolomics. The effects of differential metabolites were verified on HAdV-7 replication in an A549 cell line.

Results

The untargeted metabolomics analysis revealed more significant changes in the classes of sphingolipids, polyketides, glycerolipids, fatty acyls, and carboxylic acids and their derivatives in the patients with HAdV-7 than in healthy controls. Two key metabolic pathways of secondary and primary bile acid biosynthesis were noted from pathway enrichment analysis. Targeted metabolomics analysis showed that the levels of unconjugated bile acids in the patients were significantly lower, while the levels of glyco- and tauro- conjugated bile acids in patients and asymptomatic cases were higher than those in the healthy controls. The profiles of cytokines and peripheral lymphocyte subsets obviously varied at different levels of bile acids, with significant differences after HAdV-7 infection. A cell verification test demonstrated that the replication of HAdV-7 significantly reduced when GCDCA and TCA were added.

Conclusion

Bile acids inhibited HAdV-7 replication in vitro. Alterations in bile acids was metabolic signatures of HAdV-7 infected subjects, and our results suggested bile acids might play protective roles against HAdV-7 infection.
AKASI and Near-Infrared Spectroscopy in the combined effectiveness evaluation of an actinic keratoses preventive product in immunocompetent and immunocompromised patients
Authors: Veronese Federica , Seoni Silvia , Tarantino Vanessa , Buttafava Matteo , Airoldi Chiara , Meiburger Kristen M. , Zavattaro Elisa , Savoia Paola
Published: September 2022

Introduction

The high incidence of actinic keratoses among both the elderly population and immunocompromised subjects and the considerable risk of progression from in situ to invasive neoplasms makes it essential to identify new prevention, treatment, and monitoring strategies.

Objective

The aim of this study was to evaluate the efficacy on AKs of a topical product (®Rilastil AK Repair 100 +) containing high-protection sunscreens, a DNA Repair Complex with antioxidant and repairing action against UV-induced DNA damage, and nicotinamide, a water-soluble derivative of vitamin B3 that demonstrated several photoprotective effects both in vitro and in vivo.

Methods

The study enrolled 74 Caucasian patients, which included 42 immunocompetent and 32 immunosuppressed subjects. The efficacy of the treatment has been evaluated through the clinical index AKASI score and the non-invasive Near-Infrared Spectroscopy method.

Results

The AKASI score proved to be a valid tool to verify the efficacy of the product under study, highlighting an average percentage reduction at the end of treatment of 31.37% in immunocompetent patients and 22.76% in organ transplant recipients, in comparison to the initial values, with a statistically significant reduction also in the single time intervals (T0 vs. T1 and T1 vs. T2) in both groups. On the contrary, the Near-Infrared Spectroscopy (a non-invasive technique that evaluates hemoglobin relative concentration variations) did not find significant differences for O2Hb and HHb signals before and after the treatment, probably because the active ingredients of the product under study can repair the photo-induced cell damage, but do not significantly modify the vascularization of the treated areas.

Conclusion

The results deriving from this study demonstrate the efficacy of the product under study, confirming the usefulness of the AKASI score in monitoring treated patients. Near-Infrared Spectroscopy could represent an interesting strategy for AK patients monitoring, even if further large-scale studies will be needed.
Metagenomic analysis reveals the microbiome and antibiotic resistance genes in indigenous Chinese yellow-feathered chickens
Authors: Xu Yibin , Huang Yulin , Guo Lijin , Zhang Siyu , Wu Ruiquan , Fang Xiang , Xu Haiping , Nie Qinghua
Published: September 2022

Yellow-feathered chickens have great nutritional value and are widely and traditionally used in China, on an industrial scale as broilers. The presence of intestinal microbes has been shown to correlate with poultry performance and serves as an essential reservoir of antibiotic resistance genes (ARGs). Antibiotic resistance is a major public health concern. Here, we investigated functional characteristics of the gut microbiome of indigenous Chinese yellow-feathered chickens (the Huiyang Bearded, Xinghua, Huaixiang, Zhongshan Shanlan, Qingyuan Partridge, and Yangshan chickens) through metagenomic sequencing and reconstructed 409 draft genomes, including 60 novel species and 6 novel genera. Furthermore, we assessed the functions of the intestinal microbial communities and examined the ARGs within them. The results showed that the microbial populations of yellow-feathered broilers were primarily dominated by Bacteroidetes and Firmicutes at the phylum level and Bacteroides at the genus level. Furthermore, the Qingyuan Partridge chicken showed a significantly higher abundance of Prevotella than the other five breeds of chicken. Principal coordinates analysis indicated significant differences in the structures of microbial communities and ARGs, based on the binary Jaccard distance, among the six chicken breeds. Moreover, 989 ARGs conferring tetracycline, multidrug, and aminoglycoside resistance were identified, which represented more than 80% of the faecal resistomes; the most abundant gene in the yellow-feathered chickens was tet(Q). In addition, we found the greatest abundance of resistance genes in Xinghua chickens, indicating that Xinghua chickens are highly resistant to antibiotics. Overall, our findings revealed differences in the gut microbial community structure of indigenous Chinese yellow-feathered broiler breeds and the composition and characteristics of ARGs and antibiotic resistance that enabled us to reconstruct the yellow-feathered chicken gut microbial community genomes. The current data significantly improves our knowledge of the gut microbiome and antibiotic resistance of popular broiler breeds in China.
Covalent fragment inhibits intramembrane proteolysis
Authors: Eden Angela , Zhao Jing , Xiao Yuanyuan , Gibson James , Wang Chunyu
Published: September 2022

Alzheimer’s disease (AD) is a serious public health crisis with only one current modifying treatment. The reduction of amyloid load by targeting γ-secretase (GS) has been a leading approach in AD drug discovery and development. Despite the focus on GS inhibition, multiple GS inhibitors (GSIs) have failed in clinical trials as a result of side effects including exacerbated cognitive decline. These side effects are largely attributable to inhibition of normal GS function. Standard enzyme inhibitors target catalytic or allosteric sites of the enzyme, including the active site presenilin, as previous GSIs did. To avoid issues observed from broad-spectrum GSIs we discovered that fragment 6H8 that covalently binds to the substrate of GS, the transmembrane domain of amyloid precursor protein (APPTM). Nuclear Magnetic Resonance (NMR) Spectroscopy combined with MALDI-TOF-MS established 6H8 covalently binds to APPTM. 6H8 acts as a Michael acceptor and covalently links to the side chain amines of lysine residues, specifically targeting a cluster of C-terminal lysines K53–K55. Through this modification, 6H8 can inhibit intramembrane proteolysis of an archaeal homolog of presenilin (the active subunit of GS) via substrate binding with a 2–4 μM IC50, determined by a gel-based cleavage assay. 6H8, while too small to be an effective drug candidate, can be combined with a specific non-covalent partner and function as an effective covalent warhead of a targeted covalent inhibitor (TCI). The future development of the 6H8 fragment into the covalent warhead of a TCI is, to our knowledge, a novel approach to AD drug discovery.
Research trends and frontiers in exercise for movement disorders: A bibliometric analysis of global research from 2010 to 2021
Authors: Chen Ji-Wei , Guan Yue , Zheng Yi-Li , Zhu Kun
Published: September 2022

Objective

To conduct a bibliometric analysis of trends and frontiers on exercise-based non-pharmacological treatments for movement disorders published between 2010 and 2021.

Methods

The Web of Science (WOS) Core Collection database was searched for articles published between 2010 and 2021. The CiteSpace software was used for in-depth analysis of the countries, institutions, journals, and collaboration networks among authors and their types of articles, developmental directions, references, and hot keywords of published articles.

Results

A total of 2,626 published articles were retrieved by search formula and included in the analysis. The number of publications fluctuated during this period, with 96 countries, 3,058 institutions, and 886 academic journals having published articles in this area, with subject classifications that focused on Clinical Neurology and Neurosciences. The United States has maintained its dominant and most influential position in exercise-based non-pharmacological research on movement disorders. Among research institutions and journals, the League of European Research Universities and Movement Disorders journals published the highest number of academic articles. In the last five years, the hot research topics by burst keyword analysis, are focused on treatments, research advances, and clinical treatments.

Conclusion

Research on exercise-based non-pharmacological treatments for movement disorders is generally on the rise from 2010 to 2021. The bibliometric analysis of this area will help provide potential collaborations among researchers, frontiers, and directions for development.
Adjustment of the MIND diet tool for discriminating Greek patients with dementia: A confirmatory factor analysis
Authors: Vassilopoulou Emilia , Koumbi Lemonica , Karastogiannidou Calliope , Sotiriadis Panagiotis Marios , Felicia Pop Claudia , Tsolaki Magda
Published: September 2022

Background

The MIND diet, a hybrid of the Mediterranean and DASH diets, has been shown to reduce cognitive decline and dementia occurrence.

Aim

In the current cross-sectional study the effect of the MIND diet in elderly Greek individuals, assessed for cognitive decline, was investigated. Confirmatory factor analysis (CFA) evaluated the MIND diet score's factor structure in relation to the ability to distinguish the Greek elderly population diagnosed with or without dementia.

Methods

One hundred fifteen participants recently diagnosed with dementia and 52 cognitively healthy controls, after proper neuropsychological testing by neurologists, were included. To ensure the variance-covariance of matrix for the CFA, a second reference group of 36 participants who self-reported as healthy in terms of cognitive status from the general Greek population, was included. Demographic, anthropometric characteristics, emotional status, cognitive function, and dementia diagnosis were recorded. A prediction model investigated the MIND diet's components to separate the study participants according to their cognitive health. CFA was used to examine if the structure of the MIND diet tool scale was a proper model fit or if a different model more appropriately fit our sample data.

Results and discussion

The CFA conducted, suggested that the 9 components MIND diet score supported our sample data better than the original 15-item MIND diet.

Conclusion

The MIND diets' components must be considered in relevance to the dietary habits and cultural background of the respective population studied. Future studies should evaluate prospectively the effect of MIND-9 on preventing the onset of dementia in Greek adults.
Sleep-dependent memory consolidation in breast cancer: Use of a virtual reality prospective memory task
Authors: Duivon Mylène , Perrier Joy , Segura-Djezzar Carine , Joly Florence , Rehel Stéphane , Berthomier Christian , Grellard Jean-Michel , Clarisse Bénédicte , Geffrelot Julien , Emile George , Lévy Christelle , Viader Fausto , Eustache Francis , Desgranges Béatrice , Rauchs Géraldine , Giffard Bénédicte
Published: September 2022

Background

Previous studies have revealed both sleep alterations and prospective memory (PM) impairments in breast cancer (BC) patients. PM refers to memory of intended actions and is crucial for daily living tasks and treatment compliance. As sleep is known to favor memory consolidation, one may expect that changes in sleep quality related to BC would have an impact on PM performance. This study aimed at assessing sleep-dependent consolidation of intentions using an ecological, virtual reality-based PM task in BC patients not treated with chemotherapy.

Materials and methods

Thirty-seven early stages BC patients and 21 healthy controls (HC) participated in this study. PM was assessed using a virtual reality task, during which participants learnt a list of intentions and recalled them after a retention interval filled with a day awake or a night of sleep monitored by polysomnography. Sleep spindles and slow waves, brain oscillations involved in sleep-dependent memory consolidation, were quantified automatically using the Aseega software (Physip). Subjective sleep disturbances and markers of quality of life (psychological distress, fatigue, and well-being) were assessed by questionnaires.

Results

Greater PM performance was observed after sleep than after an equivalent period of daytime wakefulness for both groups (HC and BC). PM performance after sleep did not differ significantly between groups. Yet, BC patients reported greater sleep disturbances than HC which were related with poorer intentions retrieval, greater psychological distress, fatigue and poorer well-being. The frequency of spindles was higher and the amplitude of slow waves lower in BC patients compared to HC. However, no significant association was observed between polysomnography parameters and PM scores in the whole sample of participants.

Conclusion

Although subtle changes in brain oscillations involved in sleep-dependent memory consolidation were observed, these changes did not significantly impair overnight PM consolidation in BC patients. Nevertheless, poorer PM performance was associated with greater sleep complaints which in turn were related to poorer quality of life. Overall, these data suggest that sleep-dependent PM consolidation mechanisms are not altered in early stages BC patients not treated with chemotherapy. Further investigations are needed to understand the association between markers of quality of life and sleep-dependent memory consolidation.
Unique advantages of zebrafish larvae as a model for spinal cord regeneration
Authors: Alper Samuel R. , Dorsky Richard I.
Published: September 2022

The regenerative capacity of the spinal cord in mammals ends at birth. In contrast, teleost fish and amphibians retain this capacity throughout life, leading to the use of the powerful zebrafish model system to identify novel mechanisms that promote spinal cord regeneration. While adult zebrafish offer an effective comparison with non-regenerating mammals, they lack the complete array of experimental approaches that have made this animal model so successful. In contrast, the optical transparency, simple anatomy and complex behavior of zebrafish larvae, combined with the known conservation of pro-regenerative signals and cell types between larval and adult stages, suggest that they may hold even more promise as a system for investigating spinal cord regeneration. In this review, we highlight characteristics and advantages of the larval model that underlie its potential to provide future therapeutic approaches for treating human spinal cord injury.
Radiopharmaceutical good practices: Regulation between hospital and industry
Authors: Faivre-Chauvet Alain , Bourdeau Cécile , Bourgeois Mickaël
Published: September 2022

Radiopharmaceutical practices are divided into large-scale industrial manufacturing and small-scale “in-house” hospital radiopharmacy unit. The recent evolution of nuclear medicine involves deep consequences in this ever-present regulatory state, and hospital radiopharmacy units cannot be considered as contract manufacturing organizations (CMO). This review provides an updated status report of the official (and non-official) guidelines supporting the regulations required to meet hospital and industry common radiopharmaceutical manufacturing standards to facilitate the current and future innovative radiopharmaceutical development.
Development and validation of a nomogram to predict anastomotic leakage in colorectal cancer based on CT body composition
Authors: Xiang Shuai , Yang Yong-Kang , Wang Tong-Yu , Yang Zhi-Tao , Lu Yun , Liu Shang-Long
Published: September 2022

Background

Anastomotic leakage (AL) is one of the most serious postoperative complications. This study aimed to investigate the predictive value of preoperative body composition for AL in patients with colorectal cancer (CRC).

Methods

We first reviewed data from 3,681 patients who underwent radical CRC resection 2013–2021 in our hospital, and 60 patients were diagnosed with AL after surgery. We designed a nested case-control study and two controls were randomly selected for each case according to the time and position of surgery. Body composition was measured at the level of the third lumbar vertebra based on computed tomography (CT) images. The risk factors of AL were analyzed by univariate and multivariate analysis. Nomogram was built using binary regression analysis and assessed for clinical usefulness, calibration, and discrimination.

Results

In the multivariate analysis, gender, blood glucose, nutrition risk screening (NRS), skeletal muscle area (SMA) and visceral fat area (VFA) were independent risk factors for developing anastomotic leakage after surgery. The prognostic model had an area under the receiver operating characteristic curve of 0.848 (95% CI, 0.781–0.914). The calibration curve showed good consistency between the predicted and observed outcomes. Decision curve analysis indicated that patients with colorectal cancer can benefit from the prediction model.

Conclusions

The nomogram that combined with gender, blood glucose, NRS, SMA, and VFA had good predictive accuracy and reliability to AL. It may be conveniently for clinicians to predict AL preoperatively and be useful for guiding treatment decisions.
Food-derived bioactive oligopeptide iron complexes ameliorate iron deficiency anemia and offspring development in pregnant rats
Authors: Pan Wenfei , Gao He , Ying Xiaoling , Xu Caiju , Ye Xiang , Shao Yelin , Hua Mengdi , Shao Jie , Zhang Xinxue , Fu Shaowei , Yang Min
Published: September 2022

This study aimed to investigate anemia treatment and other potential effects of two food-derived bioactive oligopeptide iron complexes on pregnant rats with iron deficiency anemia (IDA) and their offspring. Rats with IDA were established with a low iron diet and then mated. There were one control group and seven randomly assigned groups of pregnant rats with IDA: Control group [Control, 40 ppm ferrous sulfate (FeSO4)]; IDA model group (ID, 4 ppm FeSO4), three high-iron groups (H-FeSO4, 400 ppm FeSO4; MCOP-Fe, 400 ppm marine fish oligopeptide iron complex; WCOP-Fe, 400 ppm whey protein oligopeptide iron complex) and three low-iron groups (L-FeSO4, 40 ppm FeSO4; MOP-Fe, 40 ppm marine fish oligopeptide iron complex; WOP-Fe, 40 ppm whey protein oligopeptide iron complex). Rats in each group were fed the corresponding special diet during pregnancy until the day of delivery. After different doses of iron supplement, serum hemoglobin, iron, and ferritin levels in rats with IDA were significantly increased to normal levels (P < 0.05). Serum iron levels were significantly lower in two food-derived bioactive oligopeptide low-iron complex groups than in the low FeSO4 group (P<0.05). Liver malondialdehyde levels were significantly increased in the three high-iron groups compared with the other five groups (P < 0.05), and hemosiderin deposition was observed in liver tissue, indicating that the iron dose was overloaded and aggravated the peroxidative damage in pregnant rats. Liver inflammation was reduced in the three low-iron groups. Tumor necrosis factor α secretion was significantly decreased in all groups with supplemented oligopeptide (P < 0.05), with the concentration of tumor necrosis factor α declining to normal levels in the two whey protein oligopeptide iron complex groups. In the marine fish oligopeptide iron complex groups, body length, tail length, and weight of offspring were significantly increased (P < 0.05) and reached normal levels. Therefore, food-derived bioactive oligopeptide (derived from marine fish skin and milk) iron complexes may be an effective type of iron supplement for pregnancy to improve anemia, as well as reduce the side effects of iron overload, and improve the growth and nutritional status of offspring.
Association between levels of blood trace minerals and periodontitis among United States adults
Authors: Huang Handan , Yao Jingjing , Yang Nan , Yang Liuqing , Tao Lu , Yu Jinling , Gao Ying , Liu Zhihui
Published: September 2022

Aim

Evidence linking trace minerals and periodontitis is limited. To investigate the relationship between trace minerals (selenium, manganese, lead, cadmium, and mercury) and periodontitis, data from the National Health and Nutrition Examination Survey (NHANES) were analyzed after accounting for potential confounding factors. No known studies have explored the relationship between these five trace minerals and periodontitis.

Materials and methods

A total of 4,964 participants who had undergone a full-mouth periodontal examination and laboratory tests for five trace minerals were studied in a cross-sectional study. Clinical attachment loss (CAL) and periodontitis grading were used to measure periodontitis severity. Linear and logistic regression models were used to evaluate the association between trace minerals and periodontitis. Further subgroup analyses were performed.

Results

Blood lead and cadmium levels were positively associated with mean CAL, and blood selenium was negatively associated with mean CAL; however, blood mercury, blood manganese, and mean CAL were not significantly associated. The association between trace minerals and mean CAL was more significant in males, the elderly, and patients with diabetes. There was a threshold effect between blood cadmium levels and mean CAL. Among the Black population, the relationship between blood cadmium levels and mean CAL followed an inverted U-shaped curve. There was a saturation effect in the study of blood lead in people aged 45–59 years old.

Conclusion

Our study highlighted that blood selenium, lead, and cadmium levels were significantly associated with periodontitis in a nationally representative sample of United States adults.
Vitamin D and circulating tumor cells in primary breast cancer
Authors: Mego Michal , Vlkova Barbora , Minarik Gabriel , Cierna Zuzana , Karaba Marian , Benca Juraj , Sedlackova Tatiana , Cholujova Dana , Gronesova Paulina , Kalavska Katarina , Pindak Daniel , Mardiak Jozef , Celec Peter
Published: September 2022

Background

Circulating tumor cells (CTCs) contribute to the metastatic cascade and represent an independent survival predictor in breast cancer (BC) patients. Vitamin D has pleiotropic effects, and its low concentrations are associated with breast cancer and metastasis. The aim of this study was to assess plasma vitamin D in primary BC patients in relation to CTCs.

Methods

This study included 91 non-metastatic BC patients (stage I–III) and 24 healthy donors. Blood samples for the analyses were drawn at the time of surgery. CTCs were assessed using a quantitative RT-PCR assay for expression of epithelial (CK19) or epithelial-to-mesenchymal transition (EMT) genes (TWIST1, SNAIL1, SLUG, and ZEB1). Total 25-OH vitamin D was measured in plasma using ELISA. Plasma cytokines and angiogenic factors were measured by enzyme-linked immunoassay.

Results

CTCs were detected in 30 (33%) patients. Patients with detectable CTCs in peripheral blood had significantly lower vitamin D concentrations in comparison to patients without detectable CTCs ((mean ± SD) 8.50 ± 3.89 µg/L for CTC-positive vs 9.69 ± 3.49 µg/L for CTC-negative patients, p = 0.03). The mean ( ± SD) vitamin D plasma level was 9.3 ± 3.65 µg/L for breast cancer patients compared to 18.6 ± 6.8 for healthy donors (p < 0.000001). There was no association between plasma vitamin D and other patient/tumor characteristics. Plasma vitamin D levels are inversely correlated with plasma TGF-β1, TGF-β2, IL β, IL-5, and eotaxin (all p < 0.05). Patients with vitamin D above the median had a better overall survival (hazard ratio (HR) = 0.36, 95% CI 0.16–0.80, p = 0.017), and combined analysis showed the best survival for CTC-negative patients with vitamin D levels above the median as compared to patients with opposite characteristics (HR = 0.18, 95% CI 0.05–0.63, p = 0.004).

Conclusions

Low vitamin D could be a consequence and hence a biomarker of a more invasive disease. Alternatively, vitamin D could be associated with survival because of its role in tumor dissemination. Whether its supplementation affects the metastatic cascade should be tested in animal experiments and interventional studies.