bluuebunny/crossref_metadata_2025 huggingface dataset:
Use of Dried Blood Spot Specimens to Monitor Patients with Inherited Metabolic Disorders
Authors: Moat Stuart J. , George Roanna S. , Carling Rachel S.
Published: March 2020

Monitoring of patients with inherited metabolic disorders (IMDs) using dried blood spot (DBS) specimens has been routinely used since the inception of newborn screening (NBS) for phenylketonuria in the 1960s. The introduction of flow injection analysis tandem mass spectrometry (FIA–MS/MS) in the 1990s facilitated the expansion of NBS for IMDs. This has led to increased identification of patients who require biochemical monitoring. Monitoring of IMD patients using DBS specimens is widely favoured due to the convenience of collecting blood from a finger prick onto filter paper devices in the patient’s home, which can then be mailed directly to the laboratory. Ideally, analytical methodologies with a short analysis time and high sample throughput are required to enable results to be communicated to patients in a timely manner, allowing prompt therapy adjustment. The development of ultra-performance liquid chromatography (UPLC–MS/MS), means that metabolic laboratories now have the capability to routinely analyse DBS specimens with superior specificity and sensitivity. This advancement in analytical technology has led to the development of numerous assays to detect analytes at low concentrations (pmol/L) in DBS specimens that can be used to monitor IMD patients. In this review, we discuss the pre-analytical, analytical and post-analytical variables that may affect the final test result obtained using DBS specimens used for monitoring of patients with an IMD.
Stochastic Model of Spatial Fields of the Average Daily Wind Chill Index
Authors: Kargapolova Nina
Published: March 2020

The objective of this paper was to construct a numerical stochastic model of the spatial field of the average daily wind chill index on an irregular grid defined by the location of the weather stations. It is shown in the paper that the field in question was heterogeneous and non-Gaussian. A stochastic model based on the real data collected at the weather stations located in West Siberia and on the method of the inverse distribution function that sufficiently well reproduce different characteristics of the real field of the average daily wind chill index is proposed in this paper. I also discussed several questions related to the simulation of the field on a regular grid. In the future, my intention is to transform the model proposed to a model of the conditional spatio-temporal field defined on a regular grid that allows one to forecast the wind chill index.
Electoral and Public Opinion Forecasts with Social Media Data: A Meta-Analysis
Authors: Skoric Marko M. , Liu Jing , Jaidka Kokil
Published: March 2020

In recent years, many studies have used social media data to make estimates of electoral outcomes and public opinion. This paper reports the findings from a meta-analysis examining the predictive power of social media data by focusing on various sources of data and different methods of prediction; i.e., (1) sentiment analysis, and (2) analysis of structural features. Our results, based on the data from 74 published studies, show significant variance in the accuracy of predictions, which were on average behind the established benchmarks in traditional survey research. In terms of the approaches used, the study shows that machine learning-based estimates are generally superior to those derived from pre-existing lexica, and that a combination of structural features and sentiment analyses provides the most accurate predictions. Furthermore, our study shows some differences in the predictive power of social media data across different levels of political democracy and different electoral systems. We also note that since the accuracy of election and public opinion forecasts varies depending on which statistical estimates are used, the scientific community should aim to adopt a more standardized approach to analyzing and reporting social media data-derived predictions in the future.
Nanomaterials with Tailored Magnetic Properties as Adsorbents of Organic Pollutants from Wastewaters
Authors: Peralta Marcos E. , Ocampo Santiago , Funes Israel G. , Onaga Medina Florencia , Parolo María E. , Carlos Luciano
Published: March 2020

Water quality has become one of the most critical issue of concern worldwide. The main challenge of the scientific community is to develop innovative and sustainable water treatment technologies with high efficiencies and low production costs. In recent years, the use of nanomaterials with magnetic properties used as adsorbents in the water decontamination process has received considerable attention since they can be easily separated and reused. This review focuses on the state-of-art of magnetic core–shell nanoparticles and nanocomposites developed for the adsorption of organic pollutants from water. Special attention is paid to magnetic nanoadsorbents based on silica, clay composites, carbonaceous materials, polymers and wastes. Furthermore, we compare different synthesis approaches and adsorption performance of every nanomaterials. The data gathered in this review will provide information for the further development of new efficient water treatment technologies.
Investigations of the Friction Losses of Different Engine Concepts: Part 3: Friction Reduction Potentials and Risk Assessment at the Sub-Assembly Level
Authors: Knauder Christoph , Allmaier Hannes , Sander David E. , Sams Theodor
Published: March 2020

One of the biggest requirements of today’s engine development process for passenger cars is the need to reduce fuel consumption. A very effective and economic approach is the use of low-viscosity lubricants. In this work, sub-assembly resolved friction reduction potentials and risks are presented for three different engine concepts. By using a developed combined approach, the friction losses of the base engines are separated to the sub-assemblies piston group, crankshaft journal bearings, and valve train over the full operation range of the engines. Unique analyzing of boundary conditions makes it possible for the first time to compare friction reduction potentials and possible risks, not only between diesel and gasoline engines for passenger car applications, but also with particular focus on the power density of the three engines. Firstly, the engines have been specifically chosen regarding their specific power output. Secondly, one identical SAE 5W30 lubricant suitable for all engines is used to neglect influences from different lubricant properties. Thirdly, identical test programs have been conducted at the same thermal boundary conditions at engine media supply temperatures of 70 ∘ C and 90 ∘ C. For the crankshaft journal bearings, high reduction potentials are identified, while risks arising occur at the valve train and the piston group systems.
Coalescence Dynamics of Acoustically Levitated Droplets
Authors: Hasegawa Koji , Watanabe Ayumu , Kaneko Akiko , Abe Yutaka
Published: March 2020

The contactless coalescence of a droplet is of paramount importance for physical and industrial applications. This paper describes a coalescence method to be used mid-air via acoustic levitation using an ultrasonic phased array system. Acoustic levitation using ultrasonic phased arrays provides promising lab-on-a-drop applications, such as transportation, coalescence, mixing, separation, evaporation, and extraction in a continuous operation. The mechanism of droplet coalescence in mid-air may be better understood by experimentally and numerically exploring the droplet dynamics immediately before the coalescence. In this study, water droplets were experimentally levitated, transported, and coalesced by controlled acoustic fields. We observed that the edges of droplets deformed and attracted each other immediately before the coalescence. Through image processing, the radii of curvature of the droplets were quantified and the pressure difference between the inside and outside a droplet was simulated to obtain the pressure and velocity information on the droplet’s surface. The results revealed that the sound pressure acting on the droplet clearly decreased before the impact of the droplets. This pressure on the droplets was quantitatively analyzed from the experimental data. Our experimental and numerical results provide deeper physical insights into contactless droplet manipulation for futuristic lab-on-a-drop applications.
Behavior and Mechanism of Cesium Biosorption from Aqueous Solution by Living Synechococcus PCC7002
Authors: Yu Runlan , Chai Hongsheng , Yu Zhaojing , Wu Xueling , Liu Yuandong , Shen Li , Li Jiaokun , Ye Jun , Liu Danchan , Ma Tao , Gao Fengzheng , Zeng Weimin
Published: March 2020

Many efforts have focused on the adsorption of metals from contaminated water by microbes. Synechococcus PCC7002, a major marine cyanobacteria, is widely applied to remove metals from the ocean’s photic zone. However, its ability to adsorb cesium (Cs) nuclides has received little attention. In this study, the biosorption behavior of Cs(I) from ultrapure distilled water by living Synechococcus PCC7002 was investigated based on kinetic and isotherm studies, and the biosorption mechanism was characterized by Fourier-transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectrometry, and three-dimensional excitation emission matrix fluorescence spectroscopy. Synechococcus PCC7002 showed extremely high tolerance to Cs ions and its minimal inhibitory concentration was 8.6 g/L. Extracellular polymeric substances (EPS) in Synechococcus PCC7002 played a vital role in this tolerance. The biosorption of Cs by Synechococcus PCC7002 conformed to a Freundlich-type isotherm model and pseudo-second-order kinetics. The binding of Cs(I) was primarily attributed to the extracellular proteins in EPS, with the amino, hydroxyl, and phosphate groups on the cell walls contributing to Cs adsorption. The biosorption of Cs involved two mechanisms: Passive adsorption on the cell surface at low Cs concentrations and active intracellular adsorption at high Cs concentrations. The results demonstrate that the behavior and mechanism of Cs adsorption by Synechococcus PCC7002 differ based on the Cs ions concentration.
Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults
Authors: Gargari Giorgio , Taverniti Valentina , Koirala Ranjan , Gardana Claudio , Guglielmetti Simone
Published: March 2020

The consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their impact on the intestinal microbial ecosystem. In this study, we longitudinally analyzed the bacterial taxonomic composition and organic acid levels in four fecal samples collected over the course of four weeks from 19 healthy adults who ingested one capsule a day for two weeks of a formulation containing at least 70 billion colony-forming units, consisting of 25% lactobacilli and 75% Bifidobacterium animalis subsp. lactis. We found that 16S rRNA gene profiling showed that probiotic intake only induced an increase in a single operational taxonomic unit ascribed to B. animalis, plausibly corresponding to the ingested bifidobacterial strain. Furthermore, liquid chromatography/mass spectrometry revealed a significant increase in the lactate and acetate/butyrate ratio and a trend toward a decrease in succinate following probiotic administration. The presented results indicate that the investigated probiotic formulation did not alter the intestinal bacterial ecosystem of healthy adults and suggest its potential ability to promote colonization resistance in the gut through a transient increase in fecal bifidobacteria, lactic acid, and the acetate/butyrate ratio.
Provenance of White Marbles from the Roman City of Tauriana (Palmi, Reggio Calabria, Italy)
Authors: De Luca Raffaella , Barca Donatella , Bloise Andrea , Dominici Rocco , Lezzerini Marco , Sica Maria Maddalena , Miriello Domenico
Published: March 2020

The work shows the results of an archaeometric study performed on fourteen white marble samples from the Roman city of Tauriana (Palmi, Reggio Calabria, Italy), belonging to different architectural elements of the Municipal Museum Complex and artifacts reused in the modern town. Samples were studied by optical microscopy (OM), x-ray powder diffraction (XRPD), and isotope ratio mass spectrometry (IRMS) of 13C and 18O with the aim to identify their provenance. The comparison between the collected data and the historical ones, concerning the ancient quarries of white marble of the Mediterranean area, allowed us to prove that most of the marbles used in the city of Tauriana were from the Apuan Alps Basin (Carrara) and, in few cases, from Minor Asia (Proconnesos, Aphrodisias, Docimium) and Greek (Thasos and Pentelic) quarries.
Towards Identification of Zircon Populations in Permo-Carboniferous Rhyolites of Central Europe: Insight from Automated SEM-Mineral Liberation Analyses
Authors: Przybyło Arkadiusz , Pietranik Anna , Schulz Bernhard , Breitkreuz Christoph
Published: March 2020

Zircon is a main mineral used for dating rhyolitic magmas as well as reconstructing their differentiation. It is common that different populations of zircon grains occur in a single rhyolitic sample. The presence of both autocrystic and antecrystic zircon grains is reflected in their strongly varied chemical compositions and slight spread of ages. However, postmagmatic processes may induce lead loss, which is also recorded as a spread of zircon ages. Therefore, new approaches to identify different zircon populations in rhyolitic rocks are needed. In this study, we suggest that detailed examination of zircon positions in the thin sections of rhyolitic rocks provides valuable information on zircon sources that can be used to identify autocrystic and antecrystic zircon populations. Automated Scanning Electron Microscope (SEM) analyses are of great applicability in determining this, as they return both qualitative and quantitative information and allow for quick comparisons between different rhyolite localities. Five localities of Permo-Carboniferous rhyolites related to post-Variscan extension in Central Europe (Organy, Bieberstein, Halle, Chemnitz, Krucze) were analyzed by automated SEM (MLA-SEM). The samples covered a range of Zr whole rock contents and displayed both crystalline and glassy groundmass. Surprisingly, each locality seemed to have its own special zircon fingerprint. Based on comparisons of whole rocks, modal composition and SEM images Chemnitz ignimbrite was interpreted as containing mostly (or fully) antecrystic zircon, whereas the Bieberstein dyke was shown to possibly contain both types, with the antecrystic zircon being associated with disturbed cumulates. On the other hand, Organy was probably dominated by autocrystic zircon, and Krucze contained dismembered, subhedral zircon in its matrix, whereas Halle zircon was located partly in late veins, filling cracks in laccolith. Both localities may, therefore, contain zircon populations that represent later stages than the crystallization of the main rhyolitic body.
Evaluation of Recyclability of a WEEE Slag by Means of Integrative X-Ray Computer Tomography and SEM-Based Image Analysis
Authors: Buchmann Markus , Borowski Nikolaus , Leißner Thomas , Heinig Thomas , Reuter Markus A. , Friedrich Bernd , Peuker Urs A.
Published: March 2020

Waste of electrical and electronic equipment (WEEE) is one of the fastest growing waste streams globally. Therefore, recycling of the valuable metals of this stream plays a vital role in establishing a circular economy. The smelting process of WEEE leads to significant amounts of valuable metals and rare earth elements (REEs) trapped in the slag phase. The effective manipulation of this phase transfer process necessitates detailed understanding and effective treatment to minimize these contents. Furthermore, an adequate process control to bring these metal contents into structures that make recycling economically applicable is required. Within the present study, a typical slag from a WEEE melting process is analyzed in detail. Therefore, the material is investigated with the help of X-ray computed tomography (XCT) and scanning electron microscopy (SEM)-based mineralogical analysis (MLA) to understand the typical structures and its implications for recycling. The influencing factors are discussed, and further processing opportunities are illustrated.
Exploration Potential of Fine-Fraction Heavy Mineral Concentrates from Till Using Automated Mineralogy: A Case Study from the Izok Lake Cu–Zn–Pb–Ag VMS Deposit, Nunavut, Canada
Authors: Lougheed H. Donald , McClenaghan M. Beth , Layton-Matthews Dan , Leybourne Matthew
Published: March 2020

Exploration under thick glacial sediment cover is an important facet of modern mineral exploration in Canada and northern Europe. Till heavy mineral concentrate (HMC) indicator mineral methods are well established in exploration for diamonds, gold, and base metals in glaciated terrain. Traditional methods rely on visual examination of >250 µm HMC material, however this study applies modern automated mineralogical methods (mineral liberation analysis (MLA)) to investigate the finer (<250 µm) fraction of till HMC. Automated mineralogy of finer material allows for rapid collection of precise compositional and morphological data from a large number (10,000–100,000) of heavy mineral grains in a single sample. The Izok Lake volcanogenic massive sulfide (VMS) deposit, one of the largest undeveloped Zn–Cu resources in North America, has a well-documented fan-shaped indicator mineral dispersal train and was used as a test site for this study. Axinite, a VMS indicator mineral difficult to identify optically in HMC, is identified in till samples up to 8 km down ice. Epidote and Fe-oxide minerals are identified, with concentrations peaking proximal to mineralization. Corundum and gahnite are intergrown in till samples immediately down ice of mineralization. Till samples also contain chalcopyrite and galena up to 8 km down ice of mineralization, an increase from 1.3 km for sulfide minerals in till previously reported for coarse HMC fractions. Some of these sulfide grains occur as inclusions within chemically and physically robust mineral grains and would not be identified visually in the coarse HMC visual counts. Best practices for epoxy mineral grain mounting and abundance reporting are presented along with the automated mineralogy of till samples down ice of the deposit.
Effect of Dispersion Solvent on the Deposition of PVP-Silver Nanoparticles onto DBD Plasma-Treated Polyamide 6,6 Fabric and Its Antimicrobial Efficiency
Authors: Ribeiro Ana I. , Modic Martina , Cvelbar Uros , Dinescu Gheorghe , Mitu Bogdana , Nikiforov Anton , Leys Christophe , Kuchakova Iryna , De Vrieze Mike , Felgueiras Helena P. , Souto António P. , Zille Andrea
Published: March 2020

Polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) dispersed in ethanol, water and water/alginate were used to functionalize untreated and dielectric barrier discharge (DBD) plasma-treated polyamide 6,6 fabric (PA66). The PVP-AgNPs dispersions were deposited onto PA66 by spray and exhaustion methods. The exhaustion method showed a higher amount of deposited AgNPs. Water and water-alginate dispersions presented similar results. Ethanol amphiphilic character showed more affinity to AgNPs and PA66 fabric, allowing better uniform surface distribution of nanoparticles. Antimicrobial effect in E. coli showed good results in all the samples obtained by exhaustion method but using spray method only the DBD plasma treated samples displayed antimicrobial activity (log reduction of 5). Despite the better distribution achieved using ethanol as a solvent, water dispersion samples with DBD plasma treatment displayed better antimicrobial activity against S. aureus bacteria in both exhaustion (log reduction of 1.9) and spray (methods log reduction of 1.6) due to the different oxidation states of PA66 surface interacting with PVP-AgNPs, as demonstrated by X-ray Photoelectron Spectroscopy (XPS) analysis. Spray method using the water-suspended PVP-AgNPs onto DBD plasma-treated samples is much faster, less agglomerating and uses 10 times less PVP-AgNPs dispersion than the exhaustion method to obtain an antimicrobial effect in both S. aureus and E. coli.
Understanding the Role of Temperature and Drain Current Stress in InSnZnO TFTs with Various Active Layer Thicknesses
Authors: Wang Dapeng , Furuta Mamoru , Tomai Shigekazu , Yano Koki
Published: March 2020

Thin-film transistor (TFT) devices composed of metal oxide semiconductors have attracted tremendous research attention globally in recent years. Owing to their ability to offer mobility, metal oxide semiconductor materials can enable high-performance TFTs for next-generation integrated display devices. Nevertheless, further breakthroughs of metal oxide TFTs are mainly obstructed by their long-term variability, the reason for which is not yet fully understood. Herein, TFTs based on InSnZnO (ITZO) with various thicknesses (TITZO) were prepared and their long-term stabilities under test temperatures and drain current stress were investigated. The results indicate that ITZO TFTs exhibit outstanding electrical properties regardless of the TITZO, including a high saturated mobility of over 35 cm2V−1s−1 and sharp subthreshold swing. Note that the transfer and output characteristic curves of the device with a thick TITZO of 100 nm express an abnormal current surge when high gate and drain voltages are exerted, which is attributed to the floating body effect, caused when the imposed electric field induces impact ionization near the drain side. More interestingly, these drain current stress results further suggest that the abnormal shift behavior of the electrical properties of the ITZO TFTs with a TITZO of greater than 75 nm is observed to deteriorate gradually with increasing temperature and drain current bias. This study addresses that such a degradation effect should be restrained for the operation of high-mobility devices.
Controlled Growth and Bandstructure Properties of One Dimensional Cadmium Sulfide Nanorods for Visible Photocatalytic Hydrogen Evolution Reaction
Authors: Chava Rama Krishna , Son Namgyu , Kim Yang Soo , Kang Misook
Published: March 2020

One dimensional (1D) metal sulfide nanostructures are one of the most promising materials for photocatalytic water splitting reactions to produce hydrogen (H2). However, tuning the nanostructural, optical, electrical and chemical properties of metal sulfides is a challenging task for the fabrication of highly efficient photocatalysts. Herein, 1D CdS nanorods (NRs) were synthesized by a facile and low-cost solvothermal method, in which reaction time played a significant role for increasing the length of CdS NRs from 100 nm to several micrometers. It is confirmed that as the length of CdS NR increases, the visible photocatalytic H2 evolution activity also increases and the CdS NR sample obtained at 18 hr. reaction time exhibited the highest H2 evolution activity of 206.07 μmol.g−1.h−1. The higher H2 evolution activity is explained by the improved optical absorption properties, enhanced electronic bandstructure and decreased electron-hole recombination rate.
Nanocellulose-Based Patches Loaded with Hyaluronic Acid and Diclofenac towards Aphthous Stomatitis Treatment
Authors: Carvalho João P. F. , Silva Ana C. Q. , Bastos Verónica , Oliveira Helena , Pinto Ricardo J. B. , Silvestre Armando J. D. , Vilela Carla , Freire Carmen S. R.
Published: March 2020

Nanostructured patches composed of bacterial nanocellulose (BNC), hyaluronic acid (HA) and diclofenac (DCF) were developed, envisioning the treatment of aphthous stomatitis. Freestanding patches were prepared via diffusion of aqueous solutions of HA and DCF, with different concentrations of DCF, into the wet BNC three-dimensional porous network. The resultant dual polysaccharides-based patches with a nanostructured morphology present thermal stability up to 200 °C, as well as good dynamic mechanical properties, with a storage modulus higher than 1.0 GPa. In addition, the patches are non-cytotoxic to human keratinocytes (HaCaT cells), with a cell viability of almost 100% after 24 h. The in vitro release profile of DCF from the patches was evaluated in simulated saliva, and the data refer to a diffusion- and swelling-controlled drug-release mechanism. The attained results hint at the possibility of using these dual polysaccharides-based oral mucosal patches to target aphthous stomatitis.
K+ vs. Na+ Effects on the Self-Assembly of Guanosine 5′-Monophosphate: A Solution SAXS Structural Study
Authors: Baldassarri Enrico Junior , Ortore Maria Grazia , Spinozzi Francesco , Round Adam , Ferrero Claudio , Mariani Paolo
Published: March 2020

The hierarchical process of guanosine (G) self-assembly, leading in aqueous solution and in the presence of metal cations to the formation of G-quadruplexes, represents an intriguing topic both for the biological correlation with telomerase activity and for the nano-technological applications, as demonstrated by the current measured in a quadruplex wire 100 nm long. Similar to G-rich DNA sequences and G-oligonucleotides, the guanosine 5′-monophosphate (GMP) self-aggregates in water to form quadruplexes. However, due to the absence of a covalent axial backbone, this system can be very useful to understand the chemical-physical conditions that govern the guanosine supramolecular aggregation. We have then investigated by in-solution Synchrotron Small Angle X-ray Scattering technique the role of different cations in promoting the quadruplex formation as a function of concentration and temperature. Results show how potassium, with its peculiar biological traits, favours the G-quadruplex elongation process in respect to other cations (Na + , but also NH 4 + and Li + ), determining the longest particles in solution. Moreover, the formation and the elongation of G-quadruplexes have been demonstrated to be controlled by both GMP concentration and excess cation content, even if they specifically contribute to these processes in different ways. The occurrence of condensed liquid crystalline phases was also detected, proving that excess cations play also unspecific effects on the effective charges on the G-quadruplex surface.
Piezoelectric BiFeO3 Thin Films: Optimization of MOCVD Process on Si
Authors: Micard Quentin , Condorelli Guglielmo Guido , Malandrino Graziella
Published: March 2020

This paper presents a simple and optimized metal organic chemical vapor deposition (MOCVD) protocol for the deposition of perovskite BiFeO3 films on silicon-based substrates, in order to move toward the next generation of lead-free hybrid energy harvesters. A bi-metal mixture that is composed of Bi(phenyl)3, and Fe(tmhd)3 has been used as a precursor source. BiFeO3 films have been grown by MOCVD on IrO2/Si substrates, in which the conductive IrO2 functions as a bottom electrode and a buffer layer. BiFeO3 films have been analyzed by X-ray diffraction (XRD) for structural characterization and by field-emission scanning electron microscopy (FE-SEM) coupled with energy dispersive X-ray (EDX) analysis for the morphological and chemical characterizations, respectively. These studies have shown that the deposited films are polycrystalline, pure BiFeO3 phase highly homogenous in morphology and composition all over the entire substrate surface. Piezoelectric force microscopy (PFM) and Piezoelectric Force Spectroscopy (PFS) checked the piezoelectric and ferroelectric properties of the film.
Anisotropy of Mechanical Properties of Pinctada margaritifera Mollusk Shell
Authors: Strąg Martyna , Maj Łukasz , Bieda Magdalena , Petrzak Paweł , Jarzębska Anna , Gluch Jürgen , Topal Emre , Kutukova Kristina , Clausner André , Heyn Wieland , Berent Katarzyna , Nalepka Kinga , Zschech Ehrenfried , Checa Antonio G. , Sztwiertnia Krzysztof
Published: March 2020

The mechanical properties such as compressive strength and nanohardness were investigated for Pinctada margaritifera mollusk shells. The compressive strength was evaluated through a uniaxial static compression test performed along the load directions parallel and perpendicular to the shell axis, respectively, while the hardness and Young modulus were measured using nanoindentation. In order to observe the crack propagation, for the first time for such material, the in-situ X-ray microscopy (nano-XCT) imaging (together with 3D reconstruction based on the acquired images) during the indentation tests was performed. The results were compared with these obtained during the micro-indentation test done with the help of conventional Vickers indenter and subsequent scanning electron microscopy observations. The results revealed that the cracks formed during the indentation start to propagate in the calcite prism until they reach a ductile organic matrix where most of them are stopped. The obtained results confirm a strong anisotropy of both crack propagation and the mechanical strength caused by the formation of the prismatic structure in the outer layer of P. margaritifera shell.
Morphological, Optical, and Electrical Properties of p-Type Nickel Oxide Thin Films by Nonvacuum Deposition
Authors: Diao Chien-Chen , Huang Chun-Yuan , Yang Cheng-Fu , Wu Chia-Ching
Published: March 2020

In this study, a p-type 2 at% lithium-doped nickel oxide (abbreviation L2NiO) solution was prepared using Ni(NO3)2·6H2O, and LiNO3·L2NiO thin films were deposited using an atomizer by spraying the L2NiO solution onto a glass substrate. The sprayed specimen was heated at a low temperature (140 °C) and annealed at different high temperatures and times. This method can reduce the evaporation ratio of the L2NiO solution, affording high-order nucleating points on the substrate. The L2NiO thin films were characterized by X-ray diffraction, scanning electron microscopy, UV–visible spectroscopy, and electrical properties. The figure of merit (FOM) for L2NiO thin films was calculated by Haacke’s formula, and the maximum value was found to be 5.3 × 10−6 Ω−1. FOM results revealed that the L2NiO thin films annealed at 600 °C for 3 h exhibited satisfactory optical and electrical characteristics for photoelectric device applications. Finally, a transparent heterojunction diode was successfully prepared using the L2NiO/indium tin oxide (ITO) structure. The current–voltage characteristics revealed that the transparent heterojunction diode exhibited rectifying properties, with a turn-on voltage of 1.04 V, a leakage current of 1.09 × 10−4 A/cm2 (at 1.1 V), and an ideality factor of n = 0.46.
Polyelectrolyte Based Sensors as Key to Achieve Quantitative Electronic Tongues: Detection of Triclosan on Aqueous Environmental Matrices
Authors: Magro Cátia , Zagalo Paulo , Pereira-da-Silva João , Pires Mateus Eduardo , Branco Ribeiro Alexandra , Ribeiro Paulo , Raposo Maria
Published: March 2020

Triclosan (TCS) is a bacteriostatic used in household items that promotes antimicrobial resistance and endocrine disruption effects both to humans and biota, raising health concerns. In this sense, new devices for its continuous monitoring in complex matrices are needed. In this work, sensors, based on polyelectrolyte layer-by-layer (LbL) films prepared onto gold interdigitated electrodes (IDE), were studied. An electronic tongue array, composed of (polyethyleneimine (PEI)/polysodium 4-styrenesulfonate (PSS))5 and (poly(allylamine hydrochloride/graphene oxide)5 LbL films together with gold IDE without coating were used to detect TCS concentrations (10−15–10−5 M). Electrical impedance spectroscopy was used as means of transduction and the obtained data was analyzed by principal component analysis (PCA). The electronic tongue was tested in deionized water, mineral water and wastewater matrices showing its ability to (1) distinguish between TCS doped and non-doped solutions and (2) sort out the TCS range of concentrations. Regarding film stability, strong polyelectrolytes, as (PEI/PSS)n, presented more firmness and no significant desorption when immersed in wastewater. Finally, the PCA data of gold IDE and (PEI/PSS)5 sensors, for the mineral water and wastewater matrices, respectively, showed the ability to distinguish both matrices. A sensitivity value of 0.19 ± 0.02 per decade to TCS concentration and a resolution of 0.13 pM were found through the PCA second principal component.
Enhanced Storage Stability of Different Polymer Modified Asphalt Binders through Nano-Montmorillonite Modification
Authors: Ren Zhibin , Zhu Yongqiang , Wu Qi , Zhu Minye , Guo Feng , Yu Huayang , Yu Jiangmiao
Published: March 2020

The storage stability concern, caused by phase separation for the density difference between polymers and asphalt fractions, has limited the widespread application of polymer modified asphalt (PMA). Therefore, this study aims to improve the storage concern of PMA by incorporating nano-montmorillonite. To this end, different nano-montmorillonites were incorporated to three PMAs modified with three typical asphalt modifiers, i.e., crumb rubber (CRM), styrene–butadiene-rubber (SBR) and styrene–butadiene-styrene (SBS). A series of laboratory tests were performed to evaluate the storage stability and rheological properties of PMA binders with nano-montmorillonite. As a consequence, the incorporation of nano-montmorillonite exhibited a remarkable effect on enhancing the storage stability of the CRM modified binder, but limited positive effects for the SBR and SBS modified binders. The layered nano-montmorillonite transformed to intercalated or exfoliated structures after interaction with asphalt fractions, providing superior storage stability. Among selected nano-montmorillonites, the pure montmorillonite with Hydroxyl organic ammonium performed the best on enhancing storage stability of PMA. This paper suggests that nano-montmorillonite is a promising modifier to alleviate the storage stability concern for asphalt with polymer modifiers.
Formation of Thin NiGe Films by Magnetron Sputtering and Flash Lamp Annealing
Authors: Begeza Viktor , Mehner Erik , Stöcker Hartmut , Xie Yufang , García Alejandro , Hübner Rene , Erb Denise , Zhou Shengqiang , Rebohle Lars
Published: March 2020

The nickel monogermanide (NiGe) phase is known for its electrical properties such as low ohmic and low contact resistance in group-IV-based electronics. In this work, thin films of nickel germanides (Ni–Ge) were formed by magnetron sputtering followed by flash lamp annealing (FLA). The formation of NiGe was investigated on three types of substrates: on amorphous (a-Ge) as well as polycrystalline Ge (poly-Ge) and on monocrystalline (100)-Ge (c-Ge) wafers. Substrate and NiGe structure characterization was performed by Raman, TEM, and XRD analyses. Hall Effect and four-point-probe measurements were used to characterize the films electrically. NiGe layers were successfully formed on different Ge substrates using 3-ms FLA. Electrical as well as XRD and TEM measurements are revealing the formation of Ni-rich hexagonal and cubic phases at lower temperatures accompanied by the formation of the low-resistivity orthorhombic NiGe phase. At higher annealing temperatures, Ni-rich phases are transforming into NiGe, as long as the supply of Ge is ensured. NiGe layer formation on a-Ge is accompanied by metal-induced crystallization and its elevated electrical resistivity compared with that of poly-Ge and c-Ge substrates. Specific resistivities for 30 nm Ni on Ge were determined to be 13.5 μΩ·cm for poly-Ge, 14.6 μΩ·cm for c-Ge, and 20.1 μΩ·cm for a-Ge.
An Extract of Olive Mill Wastewater Downregulates Growth, Adhesion and Invasion Pathways in Lung Cancer Cells: Involvement of CXCR4
Authors: Gallazzi Matteo , Festa Marco , Corradino Paola , Sansone Clementina , Albini Adriana , Noonan Douglas M.
Published: March 2020

Several diet-derived compounds have been reported to exert antioxidant, anti-proliferative and anti-angiogenic effects in numerous cancers and could be beneficial in cancer prevention. Olive oil production involves the generation of an aqueous phase defined as olive mill wastewater (OMWW), a polluting effluent rich in soluble polyphenols. Here, we assessed the cancer preventive properties exerted by a purified extract of OMWW (A009) for its activity on lung cancer cell lines. Hydroxytyrosol, the most abundant polyphenol present in our A009 extracts, was used as reference molecule in the assays performed. Extracts from OMWW from two different olive oil cultivars were used. We found that the A009 extracts limit lung cancer cell proliferation in a dose and time dependent manner. These effects were associated with the induction of apoptosis. A009 extracts were effective in inhibiting adhesion capabilities on a fibronectin layer accompanied with a reduction in their ability to generate invasive sprouts in a Matrigel layer. The production of chemokine CXCL12 and CXCR4 receptor were reduced by treatment with the extracts. Also, A009 interfered with the production of proangiogenic and pro-inflammatory VEGF, CXCL8, and CCL2 (as detected by FACS analysis) in the lung cell lines. A009 extracts were able to decrease STAT3 phosphorylation in lung cancer cells. Our results show that A009 extracts reduced activities related to tumor cell behavior in lung cancer cell lines, suggesting that they could have a potential cancer preventive role.
Underhydration Is Associated with Obesity, Chronic Diseases, and Death Within 3 to 6 Years in the U.S. Population Aged 51–70 Years
Authors: Stookey Jodi D. , Kavouras Stavros A. , Suh HyunGyu , Lang Florian
Published: March 2020

Nationally representative data from the National Health and Nutrition Examination Survey (NHANES) indicate that over 65% of adults aged 51–70 years in the U.S. do not meet hydration criteria. They have hyponatremia (serum sodium < 135 mmol/L) and/or underhydration (serum sodium >145 mmol/L, spot urine volume <50 mL, and/or spot urine osmolality ≥500 mmol/kg). To explore potential public health implications of not meeting hydration criteria, data from the NHANES 2009–2012 and National Center for Health Statistics Linked Mortality Files for fasting adults aged 51–70 years (sample n = 1200) were used to determine if hyponatremia and/or underhydration were cross-sectionally associated with chronic health conditions and/or longitudinally associated with chronic disease mortality. Underhydration accounted for 97% of the population group not meeting hydration criteria. In weighted multivariable adjusted Poisson models, underhydration was significantly associated with increased prevalence of obesity, high waist circumference, insulin resistance, diabetes, low HDL, hypertension, and metabolic syndrome. Over 3–6 years of follow-up, 33 chronic disease deaths occurred in the sample, representing an estimated 1,084,144 deaths in the U.S. Alongside chronic health conditions, underhydration was a risk factor for an estimated 863,305 deaths. Independent of the chronic health conditions evaluated, underhydration was a risk factor for 128,107 deaths. In weighted multivariable Cox models, underhydration was associated with 4.21 times greater chronic disease mortality (95% CI: 1.29–13.78, p = 0.019). Zero chronic disease deaths were observed for people who met the hydration criteria and did not already have a chronic condition in 2009–2012. Further work should consider effects of underhydration on population health.
Dietary Fructose Intake and Hippocampal Structure and Connectivity during Childhood
Authors: Clark Kristi A. , Alves Jasmin M. , Jones Sabrina , Yunker Alexandra G. , Luo Shan , Cabeen Ryan P. , Angelo Brendan , Xiang Anny H. , Page Kathleen A.
Published: March 2020

In rodent literature, there is evidence that excessive fructose consumption during development has a detrimental impact on hippocampal structure and function. In this study of 103 children ages 7–11 years old, we investigated whether dietary fructose intake was related to alterations in hippocampal volume and connectivity in humans. To examine if these associations were specific to fructose or were related to dietary sugars intake in general, we explored relationships between dietary intake of added sugars and the monosaccharide, glucose, on the same brain measures. We found that increased dietary intake of fructose, measured as a percentage of total calories, was associated with both an increase in the volume of the CA2/3 subfield of the right hippocampus and increased axial, radial, and mean diffusivity in the prefrontal connections of the right cingulum. These findings are consistent with the idea that increased fructose consumption during childhood may be associated with an inflammatory process, and/or decreases or delays in myelination and/or pruning. Increased habitual consumption of glucose or added sugar in general were associated with an increased volume of right CA2/3, but not with any changes in the connectivity of the hippocampus. These findings support animal data suggesting that higher dietary intake of added sugars, particularly fructose, are associated with alterations in hippocampal structure and connectivity during childhood.
Glutamine Supplementation Prevents Chronic Stress-Induced Mild Cognitive Impairment
Authors: Baek Ji Hyeong , Jung Soonwoong , Son Hyeonwi , Kang Jae Soon , Kim Hyun Joon
Published: March 2020

We recently reported that glutamine (Gln) supplementation protected glutamatergic neurotransmission from the harmful effects of chronic stress. Altered glutamatergic neurotransmission is one of the main causes of cognitive disorders. However, the cognitive enhancer function of Gln has not been clearly demonstrated thus far. Here, we evaluated whether and how Gln supplementation actually affects chronic stress-induced cognitive impairment. Using a chronic immobilization stress (CIS) mouse model, we confirmed that chronic stress induced mild cognitive impairment (MCI) and neuronal damage in the hippocampus. In contrast, Gln-supplemented mice did not show evidence of MCI. To investigate possible underlying mechanisms, we confirmed that CIS increased plasma corticosterone levels as well as brain and plasma levels of reactive oxygen/nitrogen species. CIS also increased levels of inducible nitric oxide synthase and NADPH oxidase subunits (p47phox and p67phox) in both the prefrontal cortex and CA1 region of the hippocampus. CIS decreased the number of synaptic puncta in the prefrontal cortex and hippocampus, but these effects were inhibited by Gln supplementation. Taken together, the present results suggest that Gln is an effective agent against chronic stress-induced MCI.
A Cecal Slurry Mouse Model of Sepsis Leads to Acute Consumption of Vitamin C in the Brain
Authors: Consoli David C. , Jesse Jordan J. , Klimo Kelly R. , Tienda Adriana A. , Putz Nathan D. , Bastarache Julie A. , Harrison Fiona E.
Published: March 2020

Vitamin C (ascorbate, ASC) is a critical antioxidant in the body with specific roles in the brain. Despite a recent interest in vitamin C therapies for critical care medicine, little is known about vitamin C regulation during acute inflammation and critical illnesses such as sepsis. Using a cecal slurry (CS) model of sepsis in mice, we determined ASC and inflammatory changes in the brain following the initial treatment. ASC levels in the brain were acutely decreased by approximately 10% at 4 and 24 h post CS treatment. Changes were accompanied by a robust increase in liver ASC levels of up to 50%, indicating upregulation of synthesis beginning at 4 h and persisting up to 7 days post CS treatment. Several key cytokines interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor alpha (TNFα), and chemokine (C-X-C motif) ligand 1 (CXCL1, KC/Gro) were also significantly elevated in the cortex at 4 h post CS treatment, although these levels returned to normal by 48 h. These data strongly suggest that ASC reserves are directly challenged throughout illness and recovery from sepsis. Given the timescale of this response, decreases in cortical ASC are likely driven by hyper-acute neuroinflammatory processes. However, future studies are required to confirm this relationship and to investigate how this deficiency may subsequently impact neuroinflammation.
The Effect of Alcohol on Cardiovascular Risk Factors: Is There New Information?
Authors: Minzer Simona , Losno Ricardo Arturo , Casas Rosa
Published: March 2020

The effects of alcohol on cardiovascular health are heterogeneous and vary according to consumption dose and pattern. These effects have classically been described as having a J-shaped curve, in which low-to-moderate consumption is associated with less risk than lifetime abstention, and heavy drinkers show the highest risk. Nonetheless, the beneficial effects of alcohol have been questioned due to the difficulties in establishing a safe drinking threshold. This review focuses on the association between alcohol consumption and cardiovascular risk factors and the underlying mechanisms of damage, with review of the literature from the last 10 years.
Amelioration of High-Insulin-Induced Skeletal Muscle Cell Insulin Resistance by Resveratrol Is Linked to Activation of AMPK and Restoration of GLUT4 Translocation
Authors: Vlavcheski Filip , Den Hartogh Danja J. , Giacca Adria , Tsiani Evangelia
Published: March 2020

Insulin resistance, the hallmark of type 2 diabetes mellitus (T2DM), is linked to hyperinsulinemia, which develops to counterbalance initial peripheral hormone resistance. Studies indicate that chronically elevated levels of insulin lead to skeletal muscle insulin resistance by deregulating steps within the insulin signaling cascade. The polyphenol resveratrol (RSV) has been shown to have antidiabetic properties in vitro and in vivo. In the present study, we examined the effect of RSV on high insulin (HI)-induced insulin resistance in skeletal muscle cells in vitro and investigated the mechanisms involved. Parental and GLUT4myc-overexpressing L6 rat skeletal muscle cells were used. [3H]2-deoxyglucose (2DG) uptake was measured, and total and phosphorylated levels of specific proteins were examined by immunoblotting. Exposure of L6 cells to HI levels (100 nM) for 24 h decreased the acute-insulin-stimulated 2DG uptake, indicating insulin resistance. HI increased ser307 and ser636/639 phosphorylation of IRS-1 (to 184% ± 12% and 225% ± 28.9% of control, with p < 0.001 and p < 0.01, respectively) and increased the phosphorylation levels of mTOR (174% ± 6.7% of control, p < 0.01) and p70 S6K (228% ± 33.5% of control, p < 0.01). Treatment with RSV abolished these HI-induced responses. Furthermore, RSV increased the activation of AMPK and restored the insulin-mediated increase in plasma membrane GLUT4 glucose transporter levels. These data suggest that RSV has a potential to counteract the HI-induced muscle insulin resistance.