bluuebunny/crossref_metadata_2025 huggingface dataset:
Probiotic Properties, Safety Assessment, and Aroma-Generating Attributes of Some Lactic Acid Bacteria Isolated from Iranian Traditional Cheese
Authors: Zareie Zahra , Moayedi Ali , Garavand Farhad , Tabar-Heydar Kourosh , Khomeiri Morteza , Maghsoudlou Yahya
Published: March 2023

Artisanal cheeses are known as the source of beneficial lactic acid bacteria (LAB). Therefore, this study aimed to isolate and characterize LAB with different proteolytic activities from Iranian artisanal white cheeses. The isolates were classified into low, medium, and high proteolytic activity clusters via K-means clustering and identified as Lactiplantibacillus (Lpb.) pentosus L11, Lpb. plantarum L33, and Enterococcus faecium L13, respectively. Some safety tests (such as resistance to antibiotics, hemolytic activity, and biogenic amine production), probiotic properties (including cell surface hydrophobicity, auto/co-aggregation, and antibacterial activity), and production of volatile compounds were evaluated. These were non-hemolytic and non-biogenic amine producers, and showed no irregular antibiotic resistance. Lpb. plantarum L33 had the highest hydrophobicity (30.55%) and auto-aggregation (49.56%), and the highest co-aggregation was observed for Lpb. pentosus L11 with Staphylococcus aureus (61.51%). The isolates also showed a remarkable antibacterial effect against pathogenic bacteria. Moreover, Lpb. pentosus L11 and Lpb. plantarum L33 with low and medium proteolytic activity produced a wider range of volatile compounds in milk compared to the strain with a high proteolytic effect. The results showed that a probiotic strain with low or medium proteolytic activity could improve the flavor characteristics of fermented milk.
Techno-Economic Analysis of Macroalgae Biorefineries: A Comparison between Ethanol and Butanol Facilities
Authors: Llano Tamara , Arce Carlos , Gallart Lien E. , Perales Ana , Coz Alberto
Published: March 2023

Ulva rigida seaweed is constituted by ulvan, which is a sulfated polysaccharide with uses in a wide variety of applications. After the ulvan-oriented extraction process, a crystalline and recalcitrant residue, the so-called pulp, appears. In this work, this residue was valorized through a multiple-stage process. The total processing of the algae consists of hot water extraction, acid hydrolysis, ABE fermentation, and distillation in order to obtain not only ulvan but also butanol and bioethanol to be used as biofuels by simulating two third-generation algae-based biorefineries in Aspen Plus v10 software. Third-generation plants do not compete with food and algae biomass, and they do not require delignification nor pretreatment steps, which are usually the bottleneck of second-generation plants. A plant producing butanol as biofuel together with diluted ulvan, acetone, and ethanol as byproducts was modelled in Aspen Plus software. Regarding the profitability of the investment, the plants producing bioethanol and butanol were economically feasible. The economic parameters for the bioethanol and butanol plants were as follows: NPV equal to 27.66 M$ and 16.67 M$, and IRR equal to 46% and 37%, respectively. The discounted return period was acceptable for these types of plants, which were 4.11 and 3.16 years for the ABE biorefinery and the bioethanol biorefinery, respectively.
Biocontainment Techniques and Applications for Yeast Biotechnology
Authors: Pavão Guilherme , Sfalcin Isabela , Bonatto Diego
Published: March 2023

Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as Escherichia coli account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs.
Microbial Diversity Using a Metataxonomic Approach, Associated with Coffee Fermentation Processes in the Department of Quindío, Colombia
Authors: Peñuela-Martínez Aida Esther , Velasquez-Emiliani Anyela Vanessa , Angel Carlos A.
Published: March 2023

Coffee fermentation is a complex process, mainly involving bacteria and yeasts, whose interaction influences beverage quality. The way this process is conducted affects the interactions between these microorganisms. To identify microbial diversity in fermenting coffee, samples were collected from 20 farms in the Department of Quindío, Colombia. Metataxonomic analyses using high-throughput sequencing and volatile organic compound identification in green coffee beans were performed with HS-SPME and GC-MS. Potential relationships between some families and genera with different fermentation types and coffee quality were evaluated. In our results, samples presented with high richness and diversity were greater for bacteria than for yeast/fungi. The Enterobacteriaceae family dominated at the beginning of fermentation, while Leuconostoc, Lactobacillus, Gluconobacter, and Acetobacter genera dominated at the end, a finding related to pH reduction and final coffee quality. Overall, 167 fungal families were identified, but Saccharomyceaceae dominated from the beginning. Alcohols and esters were the main chemical classes identified in green coffee bean samples from these fermentations. These results will facilitate the identification process conditions that influence the presence and abundance of microorganisms related to quality as well as contributing to the design of strategies to conduct fermentations to improve the final quality of coffee.
Numerical Investigation of Performance, Combustion, and Emission Characteristics of Various Microalgae Biodiesel on CI Engine
Authors: Rehman Madeeha , Kesharvani Sujeet , Dwivedi Gaurav
Published: March 2023

Biodiesel is being considered a possible alternative fuel due to its similarity with diesel and environmental benefits. This current work involves a numerical investigation of CI engine characteristics operating on D100 (diesel) and Dunaliella tertiolecta (DMB20), Scenedesmus obliquus (SOMB20), Scenedesmus dimorphu (SDMB20), and Chlorella protothecoides (CMB20) microalgae biodiesel blend. A diesel engine of 3.7 kW was used with variable compression ratios (CRs) (15.5, 16.5, 17.5, and 18.5) and constant speed (1500 rpm). Comparative analysis was performed for engine characteristics, including emission, combustion, and performance. Cylinder pressure, heat release rate, brake thermal efficiency, specific fuel consumption, particulate matter, oxide of nitrogen, carbon dioxide, etc., were evaluated using the blended fuel. The results show that the maximum cylinder pressure falls, SFC increases, and EGT and BTE were reduced for all blends at full load. In terms of emission characteristics, PM and smoke were lowered when compared to diesel, but a slight increment in NOx and CO2 was observed. Among all the blends, SOMB20 shows the most decrement in PM and smoke emissions by 14.16% and 11.6%, respectively, at CR 16.5. CMB20 shows a maximum increment in SFC by 3.22% at CR 17.5. A minimum reduction in CP and HRR was shown by DMB20 irrespective of CRs.
Double-Network Chitosan-Based Hydrogels with Improved Mechanical, Conductive, Antimicrobial, and Antibiofouling Properties
Authors: Carpa Rahela , Farkas Anca , Dobrota Cristina , Butiuc-Keul Anca
Published: March 2023

In recent years, the antimicrobial activity of chitosan-based hydrogels has been at the forefront of research in wound healing and the prevention of medical device contamination. Anti-infective therapy is a serious challenge given the increasing prevalence of bacterial resistance to antibiotics as well as their ability to form biofilms. Unfortunately, hydrogel resistance and biocompatibility do not always meet the demands of biomedical applications. As a result, the development of double-network hydrogels could be a solution to these issues. This review discusses the most recent techniques for creating double-network chitosan-based hydrogels with improved structural and functional properties. The applications of these hydrogels are also discussed in terms of tissue recovery after injuries, wound infection prevention, and biofouling of medical devices and surfaces for pharmaceutical and medical applications.
Evaluation of the Association between FGFR2 Gene Polymorphisms and Breast Cancer Risk in the Bangladeshi Population
Authors: Jahan Nusrat , Begum Mobashera , Barek Md Abdul , Aziz Md. Abdul , Hossen Md. Shafiul , Bhowmik Khokon Kanti , Akter Tahmina , Islam Md. Rabiul , Abdulabbas Hadi Sajid , Islam Mohammad Safiqul
Published: March 2023

Breast cancer is considered the most frequent cause of mortality from malignancy among females. Fibroblast growth factor receptor 2 (FGFR2) gene polymorphisms are highly related to the risk of breast cancer. However, no investigation has been carried out to determine the association of FGFR2 gene polymorphisms in the Bangladeshi population. Based on polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP), this study was performed to evaluate the association of FGFR2 (rs1219648, rs2420946, and rs2981582) variants in 446 Bangladeshi women (226 cases and 220 controls). A significant association of the FGFR2 rs1219648 variant with breast malignancy was reported in additive model 1 (aOR = 2.87, p < 0.0001), additive model 2 (aOR = 5.62, p < 0.0001), the dominant model (aOR = 2.87, p < 0.0001), the recessive model (aOR = 4.04, p < 0.0001), and the allelic model (OR = 2.16, p < 0.0001). This investigation also explored the significant association of the rs2981582 variant with the risk of breast cancer in additive model 2 (aOR = 2. 60, p = 0.010), the recessive model (aOR = 2.47, p = 0.006), and the allelic model (OR = 1.39, p = 0.016). However, the FGFR2 rs2420946 polymorphism showed no association with breast cancer except in the overdominant model (aOR = 0.62, p = 0.048). Furthermore, GTT (p < 0.0001) haplotypes showed a correlation with breast cancer risk, and all variants showed strong linkage disequilibrium. Moreover, in silico gene expression analysis showed that the FGFR2 level was upregulated in BC tissues compared to healthy tissues. This study confirms the association of FGFR2 polymorphisms with breast cancer risk.
Physical Activity, Mental Health, and Quality of Life among School Students in the Jazan Region of Saudi Arabia: A Cross-Sectional Survey When Returning to School after the COVID-19 Pandemic
Authors: Mahfouz Mohamed Salih , Alqassim Ahmad Y. , Sobaikhi Nasser Hadi , Jathmi Abdulaziz Salman , Alsadi Fahad Omar , Alqahtani Abdullah Mohammed , Shajri Mohammed Mohalhil , Sabi Ibrahim Darwish , Wafi Ahmed M. , Sinclair Jonathan
Published: March 2023

Increasing evidence suggests that physical activity (PA) can reduce depression and anxiety in adolescents. At the same time, quality of life (QoL) is sensitive to both mental health and PA, but little is known about the mechanism between these three variables among adolescents. This study aimed to assess the physical activity, mental health, and quality of life of school students when they returned to school after two years of distance learning in the Jazan region. This current investigation represented an observational cross-sectional survey conducted in January 2022 among a random sample of 601 students from intermediate and high schools in the Jazan region, Saudi Arabia. Three standardized questionnaires were used for data collection; the Arabic version of the Pediatric Quality of Life Inventory (PedsQL), Depression Anxiety Stress Scales (DASS21), and the Fels PAQ for children. The analysis revealed a moderate level of physical activity, decreased HRQoL, and symptoms of mental health problems (anxiety, depression, and stress) among the schools’ students when they returned to school following COVID-19 lockdown. The overall Pediatric Quality of Life mean score was (81.4 ± 16.4), which differed significantly according to gender, age groups, and grade levels (p < 0.05 for all). There was a negative correlation between the overall quality of life and mental health domains. Sport was negatively correlated with mental illness symptoms and positively correlated (p < 0.05) with Pediatric Quality of Life. The regression models revealed that stress was a significant predictor for the quality of life of male and female adolescents ([β = −0.30, (95% CI (−0.59) to (−0.02), p < 0.05)] and [β = −0.40, (95% CI (−0.70) to (−0.01), p < 0.05)], respectively). The analysis revealed a moderate level of physical activity among the schools’ students when they returned to school following COVID-19 lockdown. Children’s involvement in physical activity was associated with improved quality of life and mental health. The results call for the need to develop appropriate intervention programs to increase school students’ physical activity levels.
Volatile Organic Compounds: A Review of Their Current Applications as Pest Biocontrol and Disease Management
Authors: Razo-Belman Rosario , Ozuna César
Published: March 2023

Sustainable agriculture is the most desired solution to ensure food security for the growing world population and to face climate change. Furthermore, sustainable agriculture seeks alternatives to harmful pesticides and chemical fertilizers. This review presents an overview of recent scientific research and potential applications of volatile organic compounds (VOCs) as pest biocontrol and disease management during pre- and postharvest, along with possible limitations in scalability at the agricultural level. According to the information reviewed, bacteria, fungi, yeast, and plants are the principal organisms that produce VOCs with biotechnological potential. The main applications reported for VOCs are enhanced resistance/tolerance to abiotic stressors, such as drought, cold, and salinity, and an enhanced defense response against biotic stressors, such as viruses, bacteria, fungi, nematodes, and insects. Some VOCs in particular present an antimicrobial effect on a wide range of plant and human pathogens. Therefore, VOCs are considered a promising, sustainable biocontrol strategy that can replace pesticides and fertilizers. However, future research needs to promote collaboration with farmers and the development of applications for VOCs at the industrial level.
GA-YOLO: A Lightweight YOLO Model for Dense and Occluded Grape Target Detection
Authors: Chen Jiqing , Ma Aoqiang , Huang Lixiang , Su Yousheng , Li Wenqu , Zhang Hongdu , Wang Zhikui
Published: March 2023

Picking robots have become an important development direction of smart agriculture, and the position detection of fruit is the key to realizing robot picking. However, the existing detection models have the shortcomings of missing detection and slow detection speed when detecting dense and occluded grape targets. Meanwhile, the parameters of the existing model are too large, which makes it difficult to deploy to the mobile terminal. In this paper, a lightweight GA-YOLO model is proposed. Firstly, a new backbone network SE-CSPGhostnet is designed, which greatly reduces the parameters of the model. Secondly, an adaptively spatial feature fusion mechanism is used to address the issues of difficult detection of dense and occluded grapes. Finally, a new loss function is constructed to improve detection efficiency. In 2022, a detection experiment was carried out on the image data collected in the Bagui rural area of Guangxi Zhuang Autonomous Region, the results demonstrate that the GA-YOLO model has an mAP of 96.87%, detection speed of 55.867 FPS and parameters of 11.003 M. In comparison to the model before improvement, the GA-YOLO model has improved mAP by 3.69% and detection speed by 20.245 FPS. Additionally, the GA-YOLO model has reduced parameters by 82.79%. GA-YOLO model not only improves the detection accuracy of dense and occluded targets but also lessens model parameters and accelerates detection speed.
Systematic Analysis of Two Tandem GGDEF/EAL Domain Genes Regulating Antifungal Activities in Pseudomonas glycinae MS82
Authors: Lin Jinsheng , Qu Shaoxuan , Chen Xianyi , Li Huiping , Hou Lijuan , Lu Shi-En , Xu Ping , Jiang Ning , Ma Lin
Published: March 2023

Cyclic diguanylate (c-di-GMP) affects bacterial physiological and biochemical functions like biofilm, motility, virulence, and bacterial secretion systems. GGDEF/EAL-domain proteins, participating in c-di-GMP synthesis and degradation, are widely present in Pseudomonas, with various structures and functions. Pseudomonas glycinae MS82 is a rhizosphere bacterium that protects mushroom against the pathogenic fungi. Although 14 genes encoding GGDEF/EAL-domain proteins have been identified in the genome of MS82, c-di-GMP regulation is poorly understood as a facilitator or repressor of physiological phenotypes. Here, PafQ and PafR, encoding the proteins with the tandem GGDEF/EAL domain, were functionally analyzed and found to regulate antifungal activity. Individual deletion mutants of PafQ and PafR were constructed in P. glycinae MS82 through biparental conjugation and homologous recombination. Subsequently, antifungal activity, biofilm formation, motility, and expression of the genes related to antifungal substance synthesis were examined and contrasted with those of wild-type P. glycinae MS82. Most phenotypes of physiological activities were significantly reduced after knocking out PafQ or PafR. In other members of the genus Pseudomonas, homologous genes of PafQ and PafR possess different functions in c-di-GMP regulation. In P. glycinae, the positive regulation of PafQ and PafR on fungistatic substance synthesis, biofilm formation, and motility is crucial in the biocontrol of mushroom diseases.
Uptake and Adherence to National Guidelines on Postpartum Haemorrhage in Italy: The MOVIE before–after Observational Study
Authors: Donati Serena , Buoncristiano Marta , D’Aloja Paola , Maraschini Alice , Corsi Decenti Edoardo , Lega Ilaria ,   The ItOSS MOVIE Working Group
Published: March 2023

Translating evidence-based guidelines into clinical practice is a complex challenge. This observational study aimed to assess the adherence to the Italian national guidelines on postpartum haemorrhage (PPH) and describe the clinical management of haemorrhagic events in a selection of maternity units (MUs) in six Italian regions, between January 2019 and October 2020. A twofold study design was adopted: (i) a before–after observational study was used to assess the adherence to national clinical and organisational key recommendations on PPH management, and (ii) a cross-sectional study enrolling prospectively 1100 women with PPH ≥ 1000 mL was used to verify the results of the before–after study. The post-test detected an improved adherence to 16/17 key recommendations of the guidelines, with clinical governance and communication with family members emerging as critical areas. Overall, PPH management emerged as appropriate except for three recommended procedures that emphasise different results between the practices adopted and the difference between what is considered acquired and what is actually practised in daily care. The methodology adopted by the MOVIE project and the adopted training materials and tools have proved effective in improving adherence to the recommended procedures for appropriate PPH management and could be adopted in similar care settings in order to move evidence into practice.
Risk of Lactic Acidosis in Hospitalized Diabetic Patients Prescribed Biguanides in Japan: A Retrospective Total-Population Cohort Study
Authors: Mohri Takako , Okamoto Sawako , Nishioka Yuichi , Myojin Tomoya , Kubo Shinichiro , Higashino Tsuneyuki , Okada Sadanori , Akai Yasuhiro , Noda Tatsuya , Ishii Hitoshi , Imamura Tomoaki
Published: March 2023

Patient data from the National Database of Health Insurance Claims and Specific Health Checkups of Japan (NDB) are used to assess the effect of biguanide administration on rates of lactic acidosis (LA) in hospitalized diabetes mellitus (DM) patients. In this retrospective cohort study (from April 2013 to March 2016), we compare DM inpatients prescribed biguanides to DM inpatients who were not prescribed biguanides to quantify the association between biguanides and incidence of LA. In total, 8,111,848 DM patient records are retrieved from the NDB. Of the 528,768 inpatients prescribed biguanides, 782 develop LA. Of the 1,967,982 inpatients not prescribed biguanides, 1310 develop LA. The rate ratio of inpatients who develop LA and are administered biguanides to those who developed LA without receiving biguanides is 1.44 (95% CI, 1.32–1.58). Incidence rates and rate ratios for both sexes are elevated in the group prescribed biguanides for patients aged 70 years and older, markedly in those 80 years and older: 40.12 and 6.31 (95% CI, 4.75–8.39), respectively, for men and 34.96 and 5.40 (95% CI, 3.91–7.46), respectively, for women. Biguanides should be used conservatively in patients older than 70 years, particularly for those with comorbidities, and with caution in patients 80 years and older.
Exploring Individual Mental Health Issues: A Qualitative Study among Fellowship-Trained Sports Medicine Physicians
Authors: Stavitz James , Eckart Adam , Ghimire Pragya
Published: March 2023

The mental health of fellowship-trained sports medicine physicians (FTSMPs) around the United States is a subject that needs additional exploration. Currently, there is little research exploring how FTSMPs address their mental health on a routine basis. Using the theory of secondary trauma stress to help navigate this study, the purpose of this expressive, all-purpose qualitative study is to improve the understanding of FTSMPs’ perceptions of their mental health and the kinds of strategies used to manage these issues. This is a general qualitative study. All interviews were conducted via video communication platforms such as Zoom. The final sample included 35 FTSMPs: 25 men and 10 women. Data collection used a semi-structured interview approach. Data analysis was carried out using NVivo 12 qualitative data analysis software. Four themes emerged: mental health matters affect individual daily lives of FTSMPs; FTSMPs correlate mental health struggles with stress and anxiety; FTSMPs experience barricades when seeking support for mental health issues; and FTSMPs have poor mental health support-seeking behaviors. Results highlight openings for hospitals and private practice institutions, including producing a maintainable work–life equilibrium for FTSMPs and offering these FTSMPs access to mental health services. These recommendations may diminish exhaustion amongst several FTSMPs, a product detrimental to patients, providers, and hospitals.
Voluntary Exercise to Reduce Anxiety Behaviour in Traumatic Brain Injury Shown to Alleviate Inflammatory Brain Response in Mice
Authors: Hu Xiaoxuan , Ou Yuhang , Li Jiashuo , Sun Meiqi , Ge Qian , Pan Yongqi , Cai Zhenlu , Tan Ruolan , Wang Wenyu , An Jing , Lu Haixia
Published: March 2023

Traumatic brain injury is a leading cause of neuroinflammation and anxiety disorders in young adults. Immune-targeted therapies have garnered attention for the amelioration of TBI-induced anxiety. A previous study has indicated that voluntary exercise intervention following TBI could reduce neuroinflammation. It is essential to determine the effects of voluntary exercise after TBI on anxiety via inhibiting neuroinflammatory response. Mice were randomly divided into four groups (sham, TBI, sham + voluntary wheel running (VWR), and TBI + VWR). One-week VWR was carried out on the 2nd day after trauma. The neurofunction of TBI mice was assessed. Following VWR, anxiety behavior was evaluated, and neuroinflammatory responses in the perilesional cortex were investigated. Results showed that after one week of VWR, neurofunctional recovery was enhanced, while the anxiety behavior of TBI mice was significantly alleviated. The level of pro-inflammatory factors decreased, and the level of anti-inflammatory factors elevated. Activation of nucleotide oligomerization domain-like thermal receptor protein domain associated protein 3 (NLRP3) inflammasome was inhibited significantly. All these alterations were consistent with reduced microglial activation at the perilesional site and positively correlated with the amelioration of anxiety behavior. This suggested that timely rehabilitative exercise could be a useful therapeutic strategy for anxiety resulting from TBI by targeting neuroinflammation.
miR-33a and Its Association with Lipid Profile in Patients with Carotid Atherosclerosis
Authors: Tanashyan Marine M. , Shabalina Alla A. , Kuznetsova Polina I. , Raskurazhev Anton A.
Published: March 2023

Atherosclerosis is a chronic inflammatory disease with a complex, multifactorial pathogenesis, which includes lipid metabolism alterations. miR-33a is a microRNA that plays a key role in cholesterol efflux and promotes atherosclerosis, yet its relationship with lipid markers in carotid atherosclerosis (CA) remains unclear. The objective is to evaluate possible associations between miR-33a expression and lipid biomarkers in patients with CA. This was a prospective study that included 61 patients (median age 66.0 years, 55.7% male) with evidence of CA. Lipid profile (total cholesterol, triglycerides [TG], high-density lipoprotein [HDL] and low-density lipoprotein [LDL] cholesterol) was analyzed. Extraction and quantification of miR-33a-5p/3p was performed according to protocol. Patients were further divided depending on the target LDL level (<1.8 mmol/L). Patients with CA had relatively favorable LDL levels with a median of 2.0 mmol/L. Both miR-33a-5p and miR-33a-3p levels were lower in patients with less than targeted LDL levels (37.4 and 38.3 vs. 41.8 and 42.5 respectively, p < 0.05). A significant positive correlation between expression levels of miR-33a-5p/3p and degree of carotid stenosis was found (r = 0.44 and r = 0.38 respectively, p < 0.05). In a univariate linear regression model miR-33a-3p/5p was positively associated with LDL cholesterol (p = 0.02). miR-33a up-regulation is associated with CA and may, in fact, be a key player by targeting cholesterol metabolism. A decrease in LDL cholesterol (<1.8 mmol/L) corresponded to lower levels of miR-33a, yet the direction and causality of this association remains unclear.
Targeted Therapies in Psoriatic Arthritis—An Update
Authors: Sundanum Sonia , Orr Carl , Veale Douglas
Published: March 2023

Psoriatic arthritis (PsA) is a systemic inflammatory condition characterised by multiple clinical manifestations. Over the last decade, significant progress has been made in understanding the pathobiology of the disease. An expanded set of targeted therapies have emerged and have shown efficacy in PsA. Nevertheless, there is still a substantial subset of patients who experience no response or only a partial response to currently licensed therapies. The heterogeneous nature of the disease, together with a varying level of severity at presentation and disease activity during follow-up, brings tremendous challenges to devising management strategies. While there are certain pathophysiological similarities between PsA and rheumatoid arthritis (RA), it has become clear that there are discriminating features between these two conditions at the clinical, cellular, and molecular levels. However, there is a degree of overlap in the clinical approach when treating both PsA and RA, given that many biological and targeted therapies have proven efficacy for both pathologies. With an increasing understanding of the relevance of the IL-23/IL-17 axis in PsA, pharmacological agents blocking this pathway have provided promising possibilities for patients with PsA.
Standardized Computer-Assisted Analysis of PRAME Immunoreactivity in Dysplastic Nevi and Superficial Spreading Melanomas
Authors: Koch Elias A. T. , Erdmann Michael , Berking Carola , Kiesewetter Franklin , Kramer Rafaela , Schliep Stefan , Heppt Markus V.
Published: March 2023

PRAME (PReferentially expressed Antigen in MElanoma) is a cancer testis antigen that is frequently expressed in melanoma compared to benign melanocytic proliferations and nevi. However, the interpretation of the intensity and distribution of PRAME immunostaining is not standardized a lot, which makes interpretation difficult. PRAME-stained histological slides of superficial spreading melanomas (SSM) and dysplastic nevi (DN) were digitized and analyzed using the digital pathology and image platform QuPath. t-tests and ROC AUCs were performed with SPSS. A p-value of <0.05 was used for statistical significance, and a ROC AUC score of >0.8 was considered a good result. A cut-off score was defined in an evaluation cohort and subsequently analyzed in an independent validation cohort. In total, 81 PRAME-stained specimens were included. The evaluation cohort included 32 (50%) SSM and 32 (50%) DN, and the mean of PRAME-positive cells/mm2 for the entire lesion was 455.3 (SD 428.2) in SSM and 60.5 (SD 130.1; p < 0.001) in DN. The ROC AUC of PRAME-positive cells of the entire lesion was 0.866, and in the epidermis it was 0.901. The defined cut-off score to distinguish between DN and SSM was 97.67 cells/mm2. In the validation cohort, 16 out of 17 cases (94.1%) were correctly classified by the cut-off score. The computer-aided assessment of PRAME immunostaining is a useful tool in dermatopathology to distinguish between DN and SSM. Lesions with a moderate expression and indifferent morphologic features will remain a challenge for dermatopathologists.
Acute Ozone-Induced Transcriptional Changes in Markers of Oxidative Stress and Glucocorticoid Signaling in the Rat Hippocampus and Hypothalamus Are Sex-Specific
Authors: Valdez Matthew C. , Freeborn Danielle L. , Vulimiri Padmaja , Valdez Joseph M. , Kodavanti Urmila P. , Kodavanti Prasada Rao S.
Published: March 2023

Exposure to a prototypic air pollutant ozone (O3) has been associated with the activation of neuroendocrine stress response along with neural changes in oxidative stress (OS), inflammation, and Alzheimer’s disease-like pathologies in susceptible animal models. We hypothesized that neural oxidative and transcriptional changes induced by O3 in stress responsive regions are sex-dependent. Male and female adult Long–Evans rats were exposed to filtered air or O3 for two consecutive days (0.8 ppm, 4 h/day) and brain regions were flash-frozen. Activities of cerebellar OS parameters and mitochondrial complex I, II, and IV enzymes were assessed to confirm prior findings. We assessed transcriptional changes in hypothalamus (HYP) and hippocampus (HIP) for markers of OS, microglial activity and glucocorticoid signaling using qPCR. Although there were no O3 or sex-related differences in the cerebellar activities of OS and mitochondrial enzymes, the levels of protein carbonyls and complex II activities were higher in females regardless of O3. There were no statistical differences in baseline expression of genes related to OS (Cat, Dhcr24, Foxm1, Gpx1, Gss, Nfe2l2, Sod1) except for lower HYP Sod1 expression in air-exposed females than males, and higher HIP Gss expression in O3-exposed females relative to matched males. Microglial marker Aif1 expression was higher in O3-exposed females relative to males; O3 inhibited Itgam only in males. The expression of Bdnf in HIP and HYP was inhibited by O3 in both sexes. Genes related to glucocorticoid signaling (Fkbp4, Fkbp5, Hsp90aa1, Hspa4, nr3c1, nr3c2) showed sex-specific effects due to O3 exposure. Baseline expression of HIP Fkbp4 was higher in females relative to males. O3 inhibited Nr3c1 in female HIP and male HYP, but Nr3c2 was inhibited in male HYP. Fkbp4 expression was higher in O3-exposed females when compared to matched males, whereas Fkbp5 was expressed at higher levels in both brain regions of males and females. These results indicate that sex-specific brain region responses to O3 might, in part, be caused by OS and regulation of glucocorticoid signaling.
Propolis Ethanolic Extract Attenuates D-gal-induced C2C12 Cell Injury by Modulating Nrf2/HO-1 and p38/p53 Signaling Pathways
Authors: Tian Songhao , Zhao Huiting , Guo Hongru , Feng Wei , Jiang Conglin , Jiang Yusuo
Published: March 2023

Previous study has shown that propolis ethanolic extract (PEE) has a protective effect on aging skeletal muscle atrophy. However, the exact molecular mechanism remains unclear. This study aimed to investigate the effect of PEE on D-galactose (D-gal)-induced damage in mouse C2C12 cells. The results revealed that PEE increased the viability of senescent C2C12 cells, decreased the number of senescence-associated β-galactosidase (SA-β-Gal)-positive cells and promoted the differentiation of C2C12 cells. PEE resisted oxidative stress caused by D-gal by activating the Nrf2/HO-1 signaling pathway and maintained the differentiation ability of C2C12 cells. PEE inhibited apoptosis by suppressing p38 phosphorylation and reducing p53 expression. In summary, our findings reveal the molecular mechanism by which PEE protects D-gal-induced C2C12 cells, providing a theoretical basis for the development of PEE for the alleviation of muscle atrophy.
Increased Biomass and Polyhydroxybutyrate Production by Synechocystis sp. PCC 6803 Overexpressing RuBisCO Genes
Authors: Tharasirivat Vetaka , Jantaro Saowarath
Published: March 2023

The overexpression of the RuBisCO (rbc) gene has recently become an achievable strategy for increasing cyanobacterial biomass and overcoming the biocompound production restriction. We successfully constructed two rbc-overexpressing Synechocystis sp. PCC 6803 strains (OX), including a strain overexpressing a large subunit of RuBisCO (OXrbcL) and another strain overexpressing all large, chaperone, and small subunits of RuBisCO (OXrbcLXS), resulting in higher and faster growth than wild type under sodium bicarbonate supplementation. This increased biomass of OX strains significantly contributed to the higher polyhydroxybutyrate (PHB) production induced by nutrient-deprived conditions, in particular nitrogen (N) and phosphorus (P). As a result of higher PHB contents in OX strains occurring at days 7 and 9 of nutrient deprivation, this enhancement was apparently made possible by cells preferentially maintaining their internal lipids while accumulating less glycogen. The OXrbcLXS strain, with the highest level of PHB at about 39 %w/dry cell weight (DCW) during 7 days of BG11-NP treatment, contained a lower glycogen level (31.9 %w/DCW) than wild type control (40 %w/DCW). In contrast, the wild type control strain exposed to N- and NP-stresses tended to retain lipid levels and store more glycogen than PHB. In this model, we, for the first time, implemented a RuBisCO-overexpressing cyanobacterial factory for overproducing PHB, destined for biofuel and biomaterial biotechnology.
Synthesis and Structures of Lead(II) Complexes with Hydroxy-Substituted Closo-Decaborate Anions
Authors: Matveev Evgenii Yu. , Avdeeva Varvara V. , Kubasov Alexey S. , Zhizhin Konstantin Yu. , Malinina Elena A. , Kuznetsov Nikolay T.
Published: March 2023

Mixed-ligand lead(II) complexes with 2,2′-bipyridyl and [B10H9OH]2− or monosubstituted hydroxy-substituted closo-decaborate anions with a pendant hydroxy group, separated from the boron cage by an alkoxylic spacer of different lengths [B10H9O(CH2)xO(CH2)2OH]]2− (x = 2 or 5) have been synthesized. Compounds have been characterized by IR and multinuclear NMR spectroscopies. The structures of binuclear complex [Pb(bipy)2[B10H9OH]]2·CH3CN (1·CH3CN), mononuclear complex [Pb(bipy)2[B10H9O(CH2)2O(CH2)2OH]]·0.5bipy·CH3CN (2·0.5bipy·CH3CN), and polymeric complex [Pb(bipy)[B10H9O(CH2)5O(CH2)2OH]]n (3) have been determined by single-crystal X-ray diffraction. In all three compounds, the co-ordination polyhedra of lead(II) are formed by N atoms from two bipy molecules, O atoms of the substituent attached to the boron cage, and BH groups of the boron cage.
Life Table Study of Fall Armyworm (Spodoptera frugiperda) (Lepidoptera: Noctuidae) on Three Host Plants under Laboratory Conditions
Authors: Chen Wen-Hua , Itza Brandon , Kafle Lekhnath , Chang Tsui-Ying
Published: March 2023

After being discovered in Taiwan for the first time in June 2019, the polyphagous invasive fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), has since spread throughout the entire nation. In Taiwan, this insect has a significant impact on the quality and output of wheat, corn, sorghum, and millet. It may further infest more crops in Taiwan due to its diverse range of hosts and alternate hosts. Maize and other staple crops have already been the subject of several study. The biology of FAW has not yet been studied in relation to the alternative hosts, particularly those commonly found in Taiwanese farmlands. Therefore, this study proposed to investigate the effects of napier grass (Pennisetum purpureum), natal grass (Melinis repens), and sunn hemp (Crotalaria juncea) on the development, reproduction, survivorship, and population growth of FAW under laboratory conditions. According to the results, the developmental duration was considerably the shortest when FAW was reared on sunn hemp while the longest on natal grass. Furthermore, female adults reared on napier grass had a longer adult pre-oviposition period, total pre-oviposition period, oviposition period, longevity, highest fecundity, and highest net reproductive rate (Ro: 465.12). Among the tested three alternative host plants evaluated, sunn hemp had the highest intrinsic rate of increase (r: 0.1993), finite rate of increase (λ: 1.2206), and shortest mean generation time (T: 29.98). Therefore, this study suggests that all hosts plants can contribute to the development and outbreak of this pest in the absence of its primary host; however, sunn hemp was a relatively more suitable host plant for this insect. The possibilities for the FAW’s growth and development vary depending on the host plant. Thereby, all potential host plants in the area should be extensively examined while developing an IPM program against FAW.
Is COVID-19 All That Glitters?
Authors: Spampinato Salvatore , Di Marco Maurizio , Mammolito Luciano , Scarfia Alessia , Valastro Maurizio , Di Mauro Stefania , Bosco Giosiana , Purrello Francesco , Piro Salvatore
Published: March 2023

Over the last three years, the Coronavirus-19 disease has been a global health emergency, playing a primary role in the international scientific community. Clinical activity and scientific research have concentrated their efforts on facing the pandemic, allowing the description of novel pathologies correlated to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), such as the Multisystemic Inflammatory Syndrome in Children and Adults (MIS-C, MIS-A). Conversely, this shift of attention to COVID-19 disease and its complications could, in some cases, have delayed and underestimated the diagnosis of diseases not associated with SARS-CoV-2, including rare diseases. Here we describe the diagnostic process that led to the definition of a rare vasculitis in a young woman with a recent clinical history of SARS-CoV-2.
EASIX Is an Accurate and Easily Available Prognostic Score in Critically Ill Patients with Advanced Liver Disease
Authors: Schult David , Rasch Sebastian , Schmid Roland , Lahmer Tobias , Mayr Ulrich
Published: March 2023

Acute-on-chronic liver failure (ACLF) is associated with high mortality. Objective prognostic scores are important for treatment decisions. EASIX (Endothelial Activation and Stress Index) is a simple biomarker consisting of LDH, platelets, and creatinine, reflecting endothelial dysfunction after allogeneic stem cell transplantation. Considering endothelial dysfunction in the pathogenesis of ACLF, this study aimed to test the discriminative ability of EASIX in advanced liver disease. We retrospectively analysed the prognostic potential of EASIX to predict 28-day and 3-month mortality in a total of 188 liver cirrhotic patients requiring treatment at the intensive care unit. We evaluated the ability of EASIX to rule out early infections and predict the need for hemodialysis. EASIX performed moderately better than established scores in predicting 28-day mortality (AUC = 0.771) and was nearly equivalent (AUC = 0.791) to SOFA and APACHE-II in the prediction of 3-month mortality. Importantly, EASIX showed better diagnostic potential in ruling out clinically apparent infections than common proinflammatory markers (AUC = 0.861, p < 0.001) and showed suitable accuracy in predicting the need for hemodialysis (AUC = 0.833). EASIX is an accurate, objective and easily assessable biomarker for predicting mortality and complications in patients with advanced liver disease.
Topographical and Optical Characteristics of Thermoplastic Dental Appliances Materials Related to Water Sorption
Authors: Porojan Liliana , Toma Flavia Roxana , Bîrdeanu Mihaela Ionela , Vasiliu Roxana Diana , Matichescu Anamaria
Published: March 2023

Clear thermoplastic materials have increased in popularity in the dental field due to their various applications, combination of excellent aesthetics, and good biomechanical behavior, but they may be influenced by different environmental conditions. The purpose of the present study was to assess the topographical and optical characteristics of thermoplastic dental appliances materials relative to water sorption. PET-G polyester thermoplastic materials were evaluated in this study. Related to water uptake and desiccation stages, surface roughness was analyzed, and three-dimensional AFM profiles were generated for nano-roughness measurements. Optical CIE L*a*b* coordinates were recorded and parameters like translucency (TP), contrast ratio for the opacity (CR), and opalescence (OP) were derived. Levels of color changes were achieved. Statistical analyses were performed. Water uptake significantly increases the specific weight of the materials, and after desiccation, the mass decreases. Roughness increased after water immersion as well. Regression coefficients indicated a positive correlation between TP and a* and between OP and b*. Studied PET-G materials have a different behavior to water exposure, but for all their specific weight, they increased significantly within the first 12 h. It is accompanied by an increase in the roughness values, even if they continue to be kept below the critical mean surface roughness. On nano-level, 3D images show an increase in inhomogeneity in the network structure of particles. Slight color changes were registered.
Multilevel Multiobjective Particle Swarm Optimization Guided Superpixel Algorithm for Histopathology Image Detection and Segmentation
Authors: Kanadath Anusree , Angel Arul Jothi J. , Urolagin Siddhaling
Published: March 2023

Histopathology image analysis is considered as a gold standard for the early diagnosis of serious diseases such as cancer. The advancements in the field of computer-aided diagnosis (CAD) have led to the development of several algorithms for accurately segmenting histopathology images. However, the application of swarm intelligence for segmenting histopathology images is less explored. In this study, we introduce a Multilevel Multiobjective Particle Swarm Optimization guided Superpixel algorithm (MMPSO-S) for the effective detection and segmentation of various regions of interest (ROIs) from Hematoxylin and Eosin (H&E)-stained histopathology images. Several experiments are conducted on four different datasets such as TNBC, MoNuSeg, MoNuSAC, and LD to ascertain the performance of the proposed algorithm. For the TNBC dataset, the algorithm achieves a Jaccard coefficient of 0.49, a Dice coefficient of 0.65, and an F-measure of 0.65. For the MoNuSeg dataset, the algorithm achieves a Jaccard coefficient of 0.56, a Dice coefficient of 0.72, and an F-measure of 0.72. Finally, for the LD dataset, the algorithm achieves a precision of 0.96, a recall of 0.99, and an F-measure of 0.98. The comparative results demonstrate the superiority of the proposed method over the simple Particle Swarm Optimization (PSO) algorithm, its variants (Darwinian particle swarm optimization (DPSO), fractional order Darwinian particle swarm optimization (FODPSO)), Multiobjective Evolutionary Algorithm based on Decomposition (MOEA/D), non-dominated sorting genetic algorithm 2 (NSGA2), and other state-of-the-art traditional image processing methods.
Reducing Black–White Racial Differences on Intelligence Tests Used in Hiring for Public Safety Jobs
Authors: Goldstein Harold W. , Yusko Kenneth P. , Scherbaum Charles A. , Larson Elliott C.
Published: March 2023

This paper explores whether a diversity and inclusion strategy focused on using modern intelligence tests can assist public safety organizations in hiring a talented diverse workforce. Doing so may offer strategies for mitigating the issues of systematic racism with which these occupations have historically struggled. Past meta-analytic research shows that traditional forms of intelligence tests, which are often used in this sector, have not consistently demonstrated predictive validity but have negatively impacted Black candidates. As an alternative, we examine a modern intelligence test that consists of novel unfamiliar cognitive problems that test takers must solve without relying on their prior experience. Across six studies of varying public safety jobs (e.g., police, firefighter) in different organizations, we found a pattern of results that supports the criterion-related validity of the modern intelligence test. In addition to consistently predicting job performance and training success, the modern intelligence test also substantially mitigated the observed Black–White group differences. The implications of these findings are discussed in terms of how to alter the legacy of I/O psychology and human resource fields when it comes to our impact on facilitating employment opportunities for Black citizens, particularly in public safety positions.