bluuebunny/crossref_metadata_2025 huggingface dataset:
Leaf Phenological Responses of Juvenile Beech and Oak Provenances to Elevated Phosphorus
Authors: Bačurin Marko , Bogdan Saša , Katičić Bogdan Ida , Sever Krunoslav
Published: April 2023

The few studies dealing with leaf phenological responses to elevated nutrients in forest trees have given ambiguous results, i.e., while some reported delayed leaf-out and autumn leaf senescence, others reported advanced leaf phenology caused by increased nutrition. This study aimed to determine the effects of experimentally increased phosphorus (+P treatment) on the leaf phenologies of two juvenile provenances of common beech and sessile oak. Other objectives were to determine whether there were interspecific differences as well as intraspecific variations. Saplings were excavated in two mixed beech–oak stands and transplanted into four wooden boxes filled with a commercial soil substrate. Phosphorus fertilizer was added to two of the boxes, while the remaining boxes served as controls. Both species responded to +P treatment with advanced autumn leaf senescence in the first year of the experiment. Leaf senescence in common beech began significantly earlier, while in both species, the process was accelerated compared to that in the control. In the second year, the leaf senescence response to +P treatment was even more pronounced in both species. The +P effect on leafing phenology was absent in both common beech provenances and in an oak provenance. However, the other oak provenance showed advanced leafing, indicating the existence of intraspecific differences.
Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics
Authors: Tunkala Bereket Zeleke , DiGiacomo Kristy , Alvarez Hess Pablo S. , Dunshea Frank R. , Leury Brian J.
Published: April 2023

Storing rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed to evaluate the effect of two RF storage methods on gas composition and dry matter disappearance (DMD) in wheat grain and lucerne hay under in vitro fermentation. The storage methods were as follows: (1) snap-freezing RF using liquid nitrogen and then storing it at −80 °C (−80 °C); and (2) mixing RF with 5% dimethyl sulfoxide (DMSO), subsequently freezing it at −20 °C (D−20 °C), and comparing it to fresh RF on days 1, 14, and 30 post collection. The objective of the second phase was to quantify the impact of preserving the RF for 180 days at D−20 °C on the in vitro fermentation parameters. The methane composition was lower (p < 0.001) in both the preserved RFs than in the fresh RF. There was no difference (p < 0.05) in DMD values between days 14 and 30. The average cumulative gas production and DMD from the RF stored at D−20 °C was higher than that from the RF stored at −80 °C. Moreover, there was no difference between day 30 and day 180 in the total gas production and lag time when fermenting with RF preserved at D−20 °C. Therefore, storing RF at D−20 °C is preferable to storing it at −80 °C when access to fresh RF is limited.
Dose-Dependent Effect of Hyperoside on the Physicochemical and Gel Properties of Porcine Myofibrillar Proteins at Different NaCl Concentrations under Oxidative Stress
Authors: Guo Xiuyun , Xu Shuangyi , Meng Xiangren , Peng Zengqi
Published: April 2023

The effects of HYP (10, 50, and 250 μM/g protein) on the physicochemical and gel properties of myofibrillar proteins (MPs) at different NaCl concentrations under oxidative stress were explored. The incorporation of HYP significantly reduced carbonyl content and decreased the loss of free amine groups in a dose-dependent manner, regardless of NaCl concentration. In addition, HYP induced a dose-dependent decrement in total sulfhydryl content regardless of NaCl concentration, which might result from the formation of thiol-quinone adducts via Michael addition. The surface hydrophobicity was significantly increased with HYP addition. Nevertheless, compared with samples treated with 50 μM/g HYP, 250 μM/g HYP caused a significant decrease in surface hydrophobicity, which might be due to the increase in the extent of MPs unfolding and the concomitant aggregation of MPs by hydrophobic interaction. Furthermore, HYP also showed a dose-dependent increment in the water-holding capacity (WHC) and gel strength of MPs gels, which might be due to more orderly crosslinks via fibrous filaments at 0.2 M NaCl and more regular and lamellar structures with smaller and more homogeneous pores at 0.6 M NaCl. In summary, HYP reduced the oxidation-mediated changes of physicochemical characteristics, preventing the oxidative damage of MPs and reinforcing the ordered crosslinks of MPs–MPs and MPs–HYP during thermal gelation, ultimately resulting in a better gel quality. These results provide a theoretical support for the practical application of HYP as a natural antioxidant in gel-type meat products.
Establishment of a Sonotrode Extraction Method and Evaluation of the Antioxidant, Antimicrobial and Anticancer Potential of an Optimized Vaccinium myrtillus L. Leaves Extract as Functional Ingredient
Authors: Gil-Martínez Lidia , Aznar-Ramos María José , del Carmen Razola-Diaz Maria , Mut-Salud Nuria , Falcón-Piñeiro Ana , Baños Alberto , Guillamón Enrique , Gómez-Caravaca Ana María , Verardo Vito
Published: April 2023

Vaccinium myrtillus L. (bilberry) leaves are an important by-product of berry production that may be used as a source of phenolic compounds which have a positive effect on human health. Therefore, an ultrasound-assisted extraction via sonotrode has been used for the first time to recover bioactive compounds from bilberry leaves. The extraction has been optimized using a Box–Behnken design. The influence of ethanol:water ratio (v/v), time of extraction (min) and amplitude (%) were evaluated considering total phenolic content (TPC) and antioxidant capacity (DPPH and FRAP assays) as dependent variables in a response surface methodology (RSM). Optimum values for the independent factors were 30:70 ethanol/water (v/v), 5 min of extraction and 55% amplitude. The empirical values of the independent variables using the optimized conditions were 217.03 ± 4.92 mg GAE/g d.w. (TPC), 271.13 ± 5.84 mg TE/g d.w. (DPPH) and 312.21 ± 9.30 mg TE/g d.w. (FRAP). The validity of the experimental design was confirmed using ANOVA and the optimal extract was characterized using HPLC-MS. A total of 53 compounds were tentatively identified, of which 22 were found in bilberry leaves for the first time. Among them, chlorogenic acid was the most abundant molecule, representing 53% of the total phenolic compounds identified. Additionally, the antimicrobial and anticancer activities of the optimum extract were tested. Gram-positive bacteria demonstrated high sensitivity to bilberry leaves extract in vitro, with MBC values of 6.25 mg/mL for Listeria monocytogenes, Listeria innocua and Enterococcus faecalis, and 0.8 mg/mL for Staphylococcus aureus and Bacillus cereus. Furthermore, bilberry leaves extract exerted in vitro antiproliferative activity against HT-29, T-84 and SW-837 colon tumor cells with IC50 values of 213.2 ± 2.5, 1140.3 ± 5.2 and 936.5 ± 4.6 μg/mL, respectively. Thus, this rapid ultrasound-assisted extraction method has demonstrated to be an efficient technique to obtain bilberry leaves extract with in vitro antioxidant, antimicrobial and anticancer capacities that may be useful for the food industry as natural preservative or even for the production of functional foods or nutraceuticals.
Presence of Foodborne Bacteria in Wild Boar and Wild Boar Meat—A Literature Survey for the Period 2012–2022
Authors: Altissimi Caterina , Noé-Nordberg Clara , Ranucci David , Paulsen Peter
Published: April 2023

The wild boar is an abundant game species with high reproduction rates. The management of the wild boar population by hunting contributes to the meat supply and can help to avoid a spillover of transmissible animal diseases to domestic pigs, thus compromising food security. By the same token, wild boar can carry foodborne zoonotic pathogens, impacting food safety. We reviewed literature from 2012–2022 on biological hazards, which are considered in European Union legislation and in international standards on animal health. We identified 15 viral, 10 bacterial, and 5 parasitic agents and selected those nine bacteria that are zoonotic and can be transmitted to humans via food. The prevalence of Campylobacter, Listeria monocytogenes, Salmonella, Shiga toxin-producing E. coli, and Yersinia enterocolitica on muscle surfaces or in muscle tissues of wild boar varied from 0 to ca. 70%. One experimental study reported the transmission and survival of Mycobacterium on wild boar meat. Brucella, Coxiella burnetii, Listeria monocytogenes, and Mycobacteria have been isolated from the liver and spleen. For Brucella, studies stressed the occupational exposure risk, but no indication of meat-borne transmission was evident. Furthermore, the transmission of C. burnetii is most likely via vectors (i.e., ticks). In the absence of more detailed data for the European Union, it is advisable to focus on the efficacy of current game meat inspection and food safety management systems.
Trends in Bioactive Multilayer Films: Perspectives in the Use of Polysaccharides, Proteins, and Carbohydrates with Natural Additives for Application in Food Packaging
Authors: Avila Luisa Bataglin , Schnorr Carlos , Silva Luis F. O. , Morais Marcilio Machado , Moraes Caroline Costa , da Rosa Gabriela Silveira , Dotto Guilherme L. , Lima Éder C. , Naushad Mu.
Published: April 2023

The harmful effects on the environment caused by the indiscriminate use of synthetic plastics and the inadequate management of post-consumer waste have given rise to efforts to redirect this consumption to bio-based economic models. In this sense, using biopolymers to produce materials is a reality for food packaging companies searching for technologies that allow these materials to compete with those from synthetic sources. This review paper focused on the recent trends in multilayer films with the perspective of using biopolymers and natural additives for application in food packaging. Firstly, the recent developments in the area were presented concisely. Then, the main biopolymers used (gelatin, chitosan, zein, polylactic acid) and main methods for multilayer film preparation were discussed, including the layer-by-layer, casting, compression, extrusion, and electrospinning methods. Furthermore, we highlighted the bioactive compounds and how they are inserted in the multilayer systems to form active biopolymeric food packaging. Furthermore, the advantages and drawbacks of multilayer packaging development are also discussed. Finally, the main trends and challenges in using multilayer systems are presented. Therefore, this review aims to bring updated information in an innovative approach to current research on food packaging materials, focusing on sustainable resources such as biopolymers and natural additives. In addition, it proposes viable production routes for improving the market competitiveness of biopolymer materials against synthetic materials.
Rapid and Sensitive Detection of Sulfamethizole Using a Reusable Molecularly Imprinted Electrochemical Sensor
Authors: Kong Jie , Xu Xiaoli , Ma Yixin , Miao Junjian , Bian Xiaojun
Published: April 2023

Efficient methods for monitoring sulfonamides (SAs) in water and animal-source foods are of great importance to achieve environmental safety and protect human health. Here, we demonstrate a reusable and label-free electrochemical sensor for the rapid and sensitive detection of sulfamethizole based on an electropolymerized molecularly imprinted polymer (MIP) film as the recognition layer. To achieve effective recognition, monomer screening among four kinds of 3-substituted thiophenes was performed by computational simulation and subsequent experimental evaluation, and 3-thiopheneethanol was finally selected. MIP synthesis is very fast and green, and can be in situ fabricated on the transducer surface within 30 min in an aqueous solution. The preparation process of the MIP was characterized by electrochemical techniques. Various parameters affecting MIP fabrication and its recognition response were investigated in detail. Under optimized experimental conditions, good linearity in the range of 0.001−10 μM and a low determination limit of 0.18 nM were achieved for sulfamethizole. The sensor showed excellent selectivity, which can distinguish between structurally similar SAs. In addition, the sensor displayed good reusability and stability. Even after 7 days of storage, or being reused 7 times, higher than 90% of the initial determination signals were retained. The practical applicability of the sensor was also demonstrated in spiked water and milk samples at the nM determination level with satisfactory recoveries. Compared to relevant methods for SAs, this sensor is more convenient, rapid, economical, and eco-friendly, and had comparable or even higher sensitivity, which offered a simple and efficient method for SA detection.
Unraveling the Dysbiosis of Vaginal Microbiome to Understand Cervical Cancer Disease Etiology—An Explainable AI Approach
Authors: Sekaran Karthik , Varghese Rinku Polachirakkal , Gopikrishnan Mohanraj , Alsamman Alsamman M. , El Allali Achraf , Zayed Hatem , Doss C George Priya
Published: April 2023

Microbial Dysbiosis is associated with the etiology and pathogenesis of diseases. The studies on the vaginal microbiome in cervical cancer are essential to discern the cause and effect of the condition. The present study characterizes the microbial pathogenesis involved in developing cervical cancer. Relative species abundance assessment identified Firmicutes, Actinobacteria, and Proteobacteria dominating the phylum level. A significant increase in Lactobacillus iners and Prevotella timonensis at the species level revealed its pathogenic influence on cervical cancer progression. The diversity, richness, and dominance analysis divulges a substantial decline in cervical cancer compared to control samples. The β diversity index proves the homogeneity in the subgroups’ microbial composition. The association between enriched Lactobacillus iners at the species level, Lactobacillus, Pseudomonas, and Enterococcus genera with cervical cancer is identified by Linear discriminant analysis Effect Size (LEfSe) prediction. The functional enrichment corroborates the microbial disease association with pathogenic infections such as aerobic vaginitis, bacterial vaginosis, and chlamydia. The dataset is trained and validated with repeated k-fold cross-validation technique using a random forest algorithm to determine the discriminative pattern from the samples. SHapley Additive exPlanations (SHAP), a game theoretic approach, is employed to analyze the results predicted by the model. Interestingly, SHAP identified that the increase in Ralstonia has a higher probability of predicting the sample as cervical cancer. New evidential microbiomes identified in the experiment confirm the presence of pathogenic microbiomes in cervical cancer vaginal samples and their mutuality with microbial imbalance.
The Genetics of Fitness Reorganization during the Transition to Multicellularity: The Volvocine regA-like Family as a Model
Authors: Grochau-Wright Zachariah I. , Nedelcu Aurora M. , Michod Richard E.
Published: April 2023

The evolutionary transition from single-celled to multicellular individuality requires organismal fitness to shift from the cell level to a cell group. This reorganization of fitness occurs by re-allocating the two components of fitness, survival and reproduction, between two specialized cell types in the multicellular group: soma and germ, respectively. How does the genetic basis for such fitness reorganization evolve? One possible mechanism is the co-option of life history genes present in the unicellular ancestors of a multicellular lineage. For instance, single-celled organisms must regulate their investment in survival and reproduction in response to environmental changes, particularly decreasing reproduction to ensure survival under stress. Such stress response life history genes can provide the genetic basis for the evolution of cellular differentiation in multicellular lineages. The regA-like gene family in the volvocine green algal lineage provides an excellent model system to study how this co-option can occur. We discuss the origin and evolution of the volvocine regA-like gene family, including regA—the gene that controls somatic cell development in the model organism Volvox carteri. We hypothesize that the co-option of life history trade-off genes is a general mechanism involved in the transition to multicellular individuality, making volvocine algae and the regA-like family a useful template for similar investigations in other lineages.
Physiotherapy Screening for Referral of a Patient with Patent Foramen Ovale Presenting with Neck Pain as Primary Complaint: A Case Report
Authors: Lopez Giovanni , Cataldi Fabio , Bellin Giuseppe , Dunning James , Fernández-de-las-Peñas César , Galeno Erasmo , Meroni Roberto , Maselli Filippo , Mourad Firas
Published: April 2023

Neck pain is a common musculoskeletal disorder encountered by physiotherapists. However, it may be the early manifestation of more alarming conditions, such as cardiovascular diseases mimicking musculoskeletal pain. Patent foramen ovale (PFO) is a congenital heart defect consisting of a small opening between the right and the left atrium. A 56-year-old male presented with neck pain and head heaviness as primary complaints. The cardiovascular profile and the behavioral symptoms led the physiotherapist to find an exaggerated blood pressure response during exercise; in addition to subtle neurological signs, this prompted the physiotherapist to make an urgent referral. At the emergency department a PFO was diagnosed. To the best of the authors’ knowledge, this is the first case to describe a rare clinical presentation of a PFO presenting neck pain as primary complaint. This case report emphasizes the importance for physiotherapists to be able to triage patients for conditions outside their scope suggestive of further medical investigation.
Factors Affecting Nurse Retention Intention: With a Focus on Shift Nurses in South Korea
Authors: Cho Eun-Young , Wee Hwee
Published: April 2023

This study aims to investigate the factors affecting shift nurses’ retention to solve the nurse shortage problem. The independent variables were general characteristics, stress response, work–life balance, and grit. The subjects for the study were 214 nurses working in three shifts at three general hospitals in Korea. Data were collected from 1 to 31 August 2022. We used structured tools such as the Nurses’ Retention Index, Stress Response Inventory, Work–Life Balance Scale, and Clinical Nurse’s Grit Scale. Data analysis was conducted using descriptive statistics, independent sample t-test, one-way variance analysis, Pearson correlation analysis, and hierarchical multiple regression analysis. Age, job satisfaction, and grit were factors that had a significant impact on retention intention. Grit had the greatest impact on retention intention. Additionally, retention intention increased in those aged from 30 to 40 years as compared with those under the age of 30. It is necessary to develop and implement a program that can improve grit to enhance the retention intention of shift nurses. Additionally, it is necessary to actively seek measures to reduce dissatisfaction with nursing jobs, increase satisfaction, and manage human resources considering the characteristics of age groups.
Survival Prediction Using Transformer-Based Categorical Feature Representation in the Treatment of Diffuse Large B-Cell Lymphoma
Authors: Pant Sudarshan , Kang Sae-Ryung , Lee Minhee , Phuc Pham-Sy , Yang Hyung-Jeong , Yang Deok-Hwan
Published: April 2023

Diffuse large B-cell lymphoma (DLBCL) is a common and aggressive subtype of lymphoma, and accurate survival prediction is crucial for treatment decisions. This study aims to develop a robust survival prediction strategy to integrate various risk factors effectively, including clinical risk factors and Deauville scores in positron-emission tomography/computed tomography at different treatment stages using a deep-learning-based approach. We conduct a multi-institutional study on 604 DLBCL patients’ clinical data and validate the model on 220 patients from an independent institution. We propose a survival prediction model using transformer architecture and a categorical-feature-embedding technique that can handle high-dimensional and categorical data. Comparison with deep-learning survival models such as DeepSurv, CoxTime, and CoxCC based on the concordance index (C-index) and the mean absolute error (MAE) demonstrates that the categorical features obtained using transformers improved the MAE and the C-index. The proposed model outperforms the best-performing existing method by approximately 185 days in terms of the MAE for survival time estimation on the testing set. Using the Deauville score obtained during treatment resulted in a 0.02 improvement in the C-index and a 53.71-day improvement in the MAE, highlighting its prognostic importance. Our deep-learning model could improve survival prediction accuracy and treatment personalization for DLBCL patients.
Expanding the Use of Peroxygenase from Oat Flour in Organic Synthesis: Enantioselective Oxidation of Sulfides
Authors: Sanfilippo Claudia , Cernuto Federica , Patti Angela
Published: April 2023

Biocatalyzed oxidations are an important target in sustainable synthesis since chemical oxidations often require harsh conditions and metal-based catalysts. A raw peroxygenase-containing enzymatic preparation from oat flour was tested as a biocatalyst for the enantioselective oxidation of sulfides to sulfoxides and the variations of some reaction parameters were evaluated. Under optimal conditions, thioanisole was fully converted into the corresponding (R)-sulfoxide with high optical purity (80% ee) and the same stereopreference was maintained in the oxidation of some other sulfides. Changes in the substituent on the sulfur atom affected the selectivity of the enzyme and the best results were obtained with phenyl methoxymethyl sulfide, which gave the corresponding sulfoxide in 92% ee as exclusive product. The over-oxidation of sulfides to sulfones was instead detected in all the other cases and preferential oxidation of the (S)-enantiomer of the sulfoxide intermediate was observed, albeit with low selectivity. Carrying out the oxidation of thioanisole up to the 29% formation of sulfone led to enhancement of the sulfoxide optical purity (89% ee). The activity in sulfoxidation reactions, in addition to that reported in the epoxidation of different substrates, makes this plant peroxygenase a promising and useful tool in organic synthesis.
Factors Affecting Population Dynamics of Helicoverpa zea (Lepidoptera: Noctuidae) in a Mixed Landscape with Bt Cotton and Peanut
Authors: Santos Izailda Barbosa dos , Paula-Moraes Silvana V. , Beuzelin Julien M. , Hahn Daniel A. , Perera Omaththage P. , Fraisse Clyde
Published: April 2023

In North America, weather and host-plant abundance drive the population dynamics of the migratory pest Helicoverpa zea. The objectives of this study were to (i) estimate monthly abundance of H. zea moths in Bt cotton and peanut fields, (ii) document the effects of weather on H. zea trap catches, and (iii) determine larval hosts supporting H. zea populations from 2017 to 2019. Year-round trapping of H. zea moths was conducted in 16 commercial fields in two regions of the Florida Panhandle using delta traps. H. zea moth catches were associated with temperature, rainfall, and relative humidity. Larval hosts were determined by isotopic carbon analysis. Our results showed year-round H. zea flights in both regions across two years, with the highest and lowest moth catches occurring from July to September and November to March, respectively. There was no difference in catches between traps set on Bt cotton and peanut. In the Santa Rosa/Escambia counties, weather explained 59% of the variance in H. zea catches, with significant effects of temperature, relative humidity, and rainfall. In Jackson County, weather explained 38% of H. zea catches, with significant effects of temperature and relative humidity. Carbon isotopic data showed that feeding on C3 plants, including Bt cotton, occurred over most of the year, although feeding on C4 hosts, including Bt corn, occurred during the summer months. Hence overwintering and resident populations of H. zea in the Florida Panhandle may be continually exposed to Bt crops, increasing the risk for the evolution of resistance.
Post-Stroke Rehabilitation of Distal Upper Limb with New Perspective Technologies: Virtual Reality and Repetitive Transcranial Magnetic Stimulation—A Mini Review
Authors: Banduni Onika , Saini Megha , Singh Neha , Nath Debasish , Kumaran S. Senthil , Kumar Nand , Srivastava M. V. Padma , Mehndiratta Amit
Published: April 2023

Upper extremity motor impairment is the most common sequelae in patients with stroke. Moreover, its continual nature limits the optimal functioning of patients in the activities of daily living. Because of the intrinsic limitations in the conventional form of rehabilitation, the rehabilitation applications have been expanded to technology-driven solutions, such as Virtual Reality and Repetitive Transcranial Magnetic Stimulation (rTMS). The motor relearning processes are influenced by variables, such as task specificity, motivation, and feedback provision, and a VR environment in the form of interactive games could provide novel and motivating customized training solutions for better post-stroke upper limb motor improvement. rTMS being a precise non-invasive brain stimulation method with good control of stimulation parameters, has the potential to facilitate neuroplasticity and hence a good recovery. Although several studies have discussed these forms of approaches and their underlying mechanisms, only a few of them have specifically summarized the synergistic applications of these paradigms. To bridge the gaps, this mini review presents recent research and focuses precisely on the applications of VR and rTMS in distal upper limb rehabilitation. It is anticipated that this article will provide a better representation of the role of VR and rTMS in distal joint upper limb rehabilitation in patients with stroke.
A Monocortical Screw for Preventing Trochanteric Escape in Extended Trochanteric Osteotomy: A Simple Solution to a Complicated Problem?
Authors: Ismailidis Petros , Mündermann Annegret , Stoffel Karl
Published: April 2023

Extended trochanteric osteotomy (ETO) is an established method in revision total hip arthroplasty. Proximal migration of the greater trochanter fragment and the resulting non-union of the osteotomy remains a major problem, and several techniques have been developed to prevent its occurrence. This paper describes a novel modification of the original surgical technique in which a single monocortical screw is placed distally to one of the cerclages used for the fixation of the ETO. The contact between the screw and the cerclage counteracts the forces applied on the greater trochanter fragment and prevents trochanteric escape under the cerclage. The technique is simple and minimally invasive, does not require special skills or additional resources, or add to surgical trauma or operating time, and therefore represents a simple solution to a complicated problem.
The Association between Metabolic Syndrome and Epithelial Cell Abnormalities Detected on Pap Smear: A Nationwide Population-Based Study
Authors: Lee Dayong , Lee Taek Sang
Published: April 2023

Several epidemiologic studies have suggested the correlation between metabolic syndrome (MetS) and cervical cancer. The identification of epithelial cell abnormalities through cervical cytology implies lesions that may lead to cervical cancer in the long term, making screening a crucial measure for its prevention. We conducted a case-control study using data from the National Health Screening Programs under the Health Insurance System of South Korea between 2009 and 2017. Among women who underwent a Pap smear during this period, 8,606,394 tests reported negative results for epithelial cell abnormalities (controls, 93.7%), while 580,012 tests reported epithelial cell abnormalities (cases, 6.3%). Of these, the incidence of MetS was significantly higher in the case group, with 21.7% of cases and 18.4% of controls meeting the MetS criteria with p-Value of less than 0.0001; however, the effect size was small with odds ratio of 1.23. Logistic regression analysis revealed increased odds of epithelial cell abnormalities in women with MetS after adjusting for associated risk factors (AOR 1.202, 95% CI 1.195–1.210, p < 0.0001). These findings indicate that women with MetS have an elevated risk of developing epithelial cell abnormalities, reinforcing the importance of regular Pap smear screening to prevent cervical cancer progression in this population.
Sex-Related Disparities in Cardiac Masses: Clinical Features and Outcomes
Authors: Angeli Francesco , Bergamaschi Luca , Rinaldi Andrea , Paolisso Pasquale , Armillotta Matteo , Stefanizzi Andrea , Sansonetti Angelo , Amicone Sara , Impellizzeri Andrea , Bodega Francesca , Canton Lisa , Suma Nicole , Fedele Damiano , Bertolini Davide , Tattilo Francesco Pio , Cavallo Daniele , Di Iuorio Ornella , Ryabenko Khrystyna , Casuso Alvarez Marcello , Galiè Nazzareno , Foà Alberto , Pizzi Carmine
Published: April 2023

Background. Cardiac masses (CM) represent a heterogeneous clinical scenario, and sex-related differences of these patients remain to be established. Purpose: To evaluate sex-related disparities in CMs regarding clinical presentation and outcomes. Material and Methods. The study cohort included 321 consecutive patients with CM enrolled in our Centre between 2004 and 2022. A definitive diagnosis was achieved by histological examination or, in the case of cardiac thrombi, with radiological evidence of thrombus resolution after anticoagulant treatment. All-cause mortality at follow-up was evaluated. Multivariable regression analysis assessed the potential prognostic disparities between men and women. Results. Out of 321 patients with CM, 172 (54%) were female. Women were more frequently younger (p = 0.02) than men. Regarding CM histotypes, females were affected by benign masses more frequently (with cardiac myxoma above all), while metastatic tumours were more common in men (p < 0.001). At presentation, peripheral embolism occurred predominantly in women (p = 0.03). Echocardiographic features such as greater dimension, irregular margin, infiltration, sessile mass and immobility were far more common in men. Despite a better overall survival in women, no sex-related differences were observed in the prognosis of benign or malignant masses. In fact, in multivariate analyses, sex was not independently associated with all-cause death. Conversely, age, smoking habit, malignant tumours and peripheral embolism were independent predictors of mortality. Conclusions. In a large cohort of cardiac masses, a significant sex-related difference in histotype prevalence was found: Benign CMs affected female patients more frequently, while malignant tumours affected predominantly men. Despite better overall survival in women, sex did not influence prognosis in benign and malignant masses.
Alpha ERS-ERD Pattern during Divergent and Convergent Thinking Depends on Individual Differences on Metacontrol
Authors: Liu Chunlei , Lin Yuhong , Ye Chaoqun , Yang Jiaqin , He Wenguang
Published: April 2023

The role of metacontrol in creativity is theoretically assumed, but experimental evidence is still lacking. In this study, we investigated how metacontrol affects creativity from the perspective of individual differences. Sixty participants completed the metacontrol task, which was used to divide participants into a high-metacontrol group (HMC) versus a low (LMC) group. Then, these participants performed the alternate uses task (AUT; divergent thinking) and the remote associates test (RAT; convergent thinking), while their EEG results were recorded continuously. Regarding their behavior, the HMC group showed superior creative performance in the AUT and RAT, compared with the LMC group. For the electrophysiology, the HMC group showed larger stimulus-locked P1 and P3 amplitudes than the LMC group. Furthermore, the HMC group exhibited smaller alpha desynchronization (ERD) than the LMC group at the initial stages of the AUT task, followed by a flexible switching between alpha synchronization and desynchronization (ERS-ERD) during the process of selective retention in the AUT. In addition, the HMC group evoked smaller alpha ERD during the initial retrieval and the backtracking process in the RAT, associated with cognitive control adaptability. The aforementioned results indicate that metacontrol reliably contributes to the idea generation process, and HMC individuals could flexibly adjust their cognitive control strategies according to the demand for creative idea generation.
Improving the Accuracy of Seafloor Topography Inversion Based on a Variable Density and Topography Constraint Combined Modification Method
Authors: Sun Yongjin , Zheng Wei , Li Zhaowei , Zhou Zhiquan , Zhou Xiaocong
Published: April 2023

The use of satellite altimetry to recover marine gravity anomalies allows for the rapid acquisition of seafloor topography on a wide range of regional scales. Currently, the commonly used approaches for inverting seafloor topography have been focused on the linear correlation between gravity anomalies and seafloor topography and have disregarded the effect of density contrast between the crust and seawater on depth. Therefore, we proposed a variable density and topography constraint combined modification (VDTCCM) method by deriving Parker’s formula and the Bouguer plate formula by introducing variable density and topography factors. This method can effectively recover topography-related nonlinear terms of gravity anomalies. Subsequently, the seafloor topography of the South China Sea was estimated by applying the VDTCCM method and was defined as Seafloor topography one (ST1), and following, the accuracy was evaluated using shipborne sounding data (SSD). The results indicate that the ST1 model’s SSD-checked accuracy is 23.34% and 39.42% higher than the common international models of ETOPO1 and DTU10, respectively. Moreover, the ST1 model has advantages in mapping rugged areas, showing more detailed topographical features. Consequently, the VDTCCM method can provide beneficial references for the construction of seafloor topography models on a large regional scale using gravity anomalies recovered from satellite altimetry.
A Distributed Underwater Multi-Target Tracking Algorithm Based on Two-Layer Particle Filter
Authors: Kou Kunhu , Li Bochen , Ding Lu , Song Lei
Published: April 2023

Underwater multi-target tracking is one of the key technologies for military missions, including patrol and combat in the crucial area. Since the underwater environment is complex and targets’ trajectories may intersect when they are in a dense area, it is challenging to guarantee the precision of observed information. In order to provide high-precision underwater localization and tracking services over an underwater monitoring network, a dynamic network resource allocation mechanism and an underwater multi-target tracking algorithm based on a two-layer particle filter with distributed probability fusion (TLPF-DPF) are proposed. The position estimation model based on geometric constraints and the dynamic allocation mechanism of network resources based on prior position estimation are designed. Using the improved filtering algorithm with known initial states, the reliable tracking of multiple targets with trajectory intersection in a small area under complex noises is achieved. In the non-Gaussian environment, the average positioning error of TLPF-DPF is less by nearly 30% than alternative algorithms. When switching from a Gaussian environment to a non-Gaussian environment, the performance degradation of TLPF-DPF is less than 12%, which exhibits stability compared with other algorithms when targets are close to each other with crossing trajectories.
The First Whole Genome Sequencing of Agaricus bitorquis and Its Metabolite Profiling
Authors: Zhao Chunhua , Feng Xi-long , Wang Zhen-xin , Qi Jianzhao
Published: April 2023

Agaricus bitorquis, an emerging wild mushroom with remarkable biological activities and a distinctive oversized mushroom shape, has gained increasing attention in recent years. Despite its status as an important resource of wild edible fungi, knowledge about this mushroom is still limited. In this study, we used the Illumina NovaSeq and Nanopore PromethION platforms to sequence, de novo assemble, and annotate the whole genome and mitochondrial genome (mitogenome) of the A. bitorquis strain BH01 isolated from Bosten Lake, Xinjiang Province, China. Using the genome-based biological information, we identified candidate genes associated with mating type and carbohydrate-active enzymes in A. bitorquis. Cluster analysis based on P450 of basidiomycetes revealed the types of P450 members of A. bitorquis. Comparative genomic, mitogenomic, and phylogenetic analyses were also performed, revealing interspecific differences and evolutionary features of A. bitorquis and A. bisporus. In addition, the molecular network of metabolites was investigated, highlighting differences in the chemical composition and content of the fruiting bodies of A. bitorquis and A. bisporus. The genome sequencing provides a comprehensive understanding and knowledge of A. bitorquis and the genus Agaricus mushrooms. This work provides valuable insights into the potential for artificial cultivation and molecular breeding of A. bitorquis, which will facilitate the development of A. bitorquis in the field of edible mushrooms and functional food manufacture.
Physicians’ Perspectives on a Multi-Dimensional Model for the Roles of Electronic Health Records in Approaching a Proper Differential Diagnosis
Authors: Alanazi Abdullah , Almutib Amal , Aldosari Bakheet
Published: April 2023

Many healthcare organizations have adopted Electronic Health Records (EHRs) to improve the quality of care and help physicians make proper clinical decisions. The vital roles of EHRs can support the accuracy of diagnosis, suggest, and rationalize the provided care to patients. This study aims to understand the roles of EHRs in approaching proper differential diagnosis and optimizing patient safety. This study utilized a cross-sectional survey-based descriptive research design to assess physicians’ perceptions of the roles of EHRs on diagnosis quality and safety. Physicians working in tertiary hospitals in Saudi Arabia were surveyed. Three hundred and fifty-one participants were included in the study, of which 61% were male. The main participants were family/general practice (22%), medicine, general (14%), and OB/GYN (12%). Overall, 66% of the participants ranked themselves as IT competent, most of the participants underwent IT self-guided learning, and 65% of the participants always used the system. The results generally reveal positive physicians’ perceptions toward the roles of the EHR system on diagnosis quality and safety. There was a statistically significant relationship between user characteristics and the roles of the EHR by enhancing access to care, patient–physician encounter, clinical reasoning, diagnostic testing and consultation, follow-up, and diagnostic safety functionality. The study participants demonstrate positive perceptions of physicians toward the roles of the EHR system in approaching differential diagnosis. Yet, areas of improvement in the design and using EHRs are emphasized.
Coupling the Empirical Wavelet and the Neural Network Methods in Order to Forecast Electricity Price
Authors: Boubaker Heni , Bannour Nawres
Published: April 2023

This paper aims to evaluate the forecast capability of electricity markets, categorized by numerous major characteristics such as non-stationarity, nonlinearity, highest volatility, high frequency, mean reversion and multiple seasonality, which give multifarious forecasts. To improve it, this investigation proposes a new hybrid approach that links a dual long-memory process (Gegenbauer autoregressive moving average (GARMA) and generalized long-memory GARCH (G-GARCH)) and the empirical wavelet transform (EWT) and local linear wavelet neural network (LLWNN) approaches, forming the k-factor GARMA-EWLLWNN model. The future hybrid model accomplished is assessed via data from the Polish electricity markets, and it is matched with the generalized long-memory k-factor GARMA-G-GARCH process and the hybrid EWLLWNN, to demonstrate the robustness of our approach. The obtained outcomes show that the suggested model presents important results to define the relevance of the modeling approach that offers a remarkable framework to reproduce the inherent characteristics of the electricity prices. Finally, it is presented that the adopted methodology is the most appropriate one for prediction as it realizes a better prediction performance and may be an answer for forecasting electricity prices.
Study on the Effect of Job Accessibility and Residential Location on Housing Occupancy Rate: A Case Study of Xiamen, China
Authors: Ren Feng , Zhang Jinbo , Yang Xiuyun
Published: April 2023

The serious mismatch between industrialization and urbanization has led to the emergence of ghost cities. Industry-and-city integration aims to agglomerate industries and the population simultaneously by coordinating the planning and construction, and by mixing different functional areas including industry, office, living, and commercial functions. Based on the population spatial vector database of Jimei District in Xiamen in 2020, this paper empirically analyzes the effects of spatial patterns between industry and city, in terms of residential location and job accessibility, on the housing occupancy rate in new towns and cities. The findings demonstrate that: (1) The attraction of residential location to population varies among three different urban expansion models. The housing occupancy rate of residential areas that meet the concentric circle model is the highest, followed by the sector model, and the multiple nuclei model is the lowest; (2) The jobs–housing relationship has a stable and positive impact on the occupancy rate of commercial housing in the new town, which verifies that job accessibility is the basic demand for families’ residential location choice; (3) There is a significant pattern difference in the influence of job accessibility on the occupancy rate. The occupancy rate of the sector model residential area is highly dependent on job accessibility: the higher the job accessibility, the lower the occupancy rate of the concentric residential area, while job accessibility has a weak impact on the occupancy rate of the multiple nuclei residential area. The conclusions suggest that the spatial planning of new towns should include a clear population absorbing strategy, and the residential location should follow the expansion law of the urban residential functional area, balance the relationship between industrial agglomeration and the job–housing relationship, and allocate life factors in a targeted manner according to the actual impact of job accessibility.
Chemical Weathering Rates of Soils Developed on Eocene Marls and Sandstones in a Mediterranean Catchment (Istria, Croatia)
Authors: Hasan Ozren , Miko Slobodan , Mesić Saša , Peh Zoran
Published: April 2023

Physical and chemical weathering, together with biological and biochemical processes, form soil from bedrock and strongly influence the chemical composition of natural waters. Erosive processes, primarily through the agents of running water and wind, remove the products of weathering from catchments. The aim was to determine the chemical weathering of minerals because of changes in land-use and natural forestation in two small neighboring catchments of the rivers Argilla and Bazuja. Agricultural land-use practice is very intense in the Argilla catchment, while the Bazuja catchment’s arable land is mostly abandoned, with progressive forestation. Chemical weathering in soils and sediments was evaluated with the aid of bulk chemistry analysis focused on major elements, trace elements, and zirconium. Weathering indices, mass balance, and strain were calculated. The abandonment of arable land and intense forestation in the Bazuja catchment caused increased chemical weathering with the loss of base cations (Ca and Mg) and enrichment of conservative elements (Zr and Ti) in surface horizons. EIC and MTF values are positive (enrichment) in areas with agricultural activities, while forested areas show negative values (loss). A comparison of the oldest and youngest parts of the overbank sediment profiles in the swallow hole zone and stream sediments shows that chemical and mechanical weathering in the Bazuja catchment was similar to present weathering in the Argilla catchment, while agriculture was active in the Bazuja catchment. The integrated knowledge gained in small catchment studies can be broadly applicable to larger systems.
The Influence of Focus on the Activation of Alternatives in Speech Production—An Online Picture-Word-Interference Experiment
Authors: Bergmann Beate , Lu Yanru , Spalek Katharina
Published: April 2023

In previous picture naming tasks, semantically related distractor words (co-hyponyms to the target word) induce interference, which is usually taken as evidence for lexical competition. In an online picture-word-interference experiment, we showed that distractor words that share a feature with the target (here: their natural prototypical color), also induced interference. Pictures were not named with single words but with short descriptive sentences (“The heart is red”). Focus on the noun modulated the interference effect. In particular, when target and distractor were presented simultaneously, the interference effect was significantly reduced in the narrow focus condition, compared to broad focus. We discuss our findings for focus production against the findings on language comprehension reported in the literature, which mostly observed facilitatory effects of focus marking on the comprehension of focus alternatives.
Pramipexole Augmentation for Treatment-Resistant Unipolar and Bipolar Depression in the Real World: A Systematic Review and Meta-Analysis
Authors: Tundo Antonio , Betro’ Sophia , de Filippis Rocco , Marchetti Fulvia , Nacca Daniele , Necci Roberta , Iommi Marica
Published: April 2023

Background: Pramipexole is a dopamine full agonist approved for the treatment of Parkinson’s disease and restless legs syndrome. Its high affinity for the D3 receptor and neuroprotective, antioxidant, and anti-inflammatory activity provides a rationale for the treatment of depression. In this paper, we review studies on the effectiveness and safety of antidepressant pramipexole augmentation in treatment-resistant depression. Methods: This comprehensive systematic review and meta-analysis of observational studies on pramipexole–antidepressant augmentation included patients with resistant unipolar and bipolar depression. The primary outcome measure was the treatment response, measured at the study endpoint. Results: We identified 8 studies including 281 patients overall, 57% women and 39.5% with bipolar disorder and 60.5% with major depressive disorder. The mean follow-up duration was 27.3 weeks (range 8–69). The pooled estimate of treatment response was 62.5%, without significant differences between unipolar and bipolar depression. Safety was good, with nausea and somnolence the most frequent side effects. Conclusions: The findings of this systematic review, needing further confirmation, show that off-label use of pramipexole as augmentation of antidepressant treatment could be a useful and safe strategy for unipolar and bipolar treatment-resistant depression.