bluuebunny/crossref_metadata_2025 huggingface dataset:
A multi-methodological approach to record dynamics and timescales of the plumbing system of Zaro (Ischia Island, Italy)
Authors: Pelullo Carlo , Chakraborty Sumit , Montagna Chiara Paola , Arienzo Ilenia , Brown Richard James , D’Antonio Massimo , de Vita Sandro , D’Oriano Claudia , Nazzari Manuela , Pappalardo Lucia , Petrosino Paola
Published: May 2024

Determining the time spans of processes related to the assembly of eruptible magma at active volcanoes is fundamental to understand magma chamber dynamics and assess volcanic hazard. This information can be recorded in the chemical zoning of crystals. Nevertheless, this kind of study is still poorly employed for the active volcanoes of the Neapolitan area (Southern Italy), in particular, for Ischia island where the risk is extremely high and this information can provide the basis for probabilistic volcanic hazard assessment. For these reasons, we acquired chemical composition on clinopyroxene crystals erupted at Ischia during the Zaro eruption (6.6 ± 2.2 ka) and performed numerical simulations of the input of mafic magma into a trachytic reservoir, in order to investigate various aspects of pre-eruptive dynamics occurring at different timescales. This event emplaced a ~ 0.1 km3 lava complex, in which the main trachytic lava flows host abundant mafic to felsic enclaves. Previous petrological investigation suggested that mafic magma(s) mixed/mingled with a trachytic one, before the eruption. In this work, the clinopyroxene zoning patterns depict the growth of crystals in different magmatic environments, recording sequential changes occurred in the plumbing system before the eruption. The evolution of the plumbing system involved a hierarchy of timescales: a few hours for magma mingling caused by mafic recharge(s) and likely occurred multiple times over a decade during which a dominant magmatic environment was sustained before the eruption. Such timescales must be considered in volcanic hazard assessment at Ischia and similar active volcanoes in densely populated areas.
LAMC2 regulates the proliferation, invasion, and metastasis of gastric cancer via PI3K/Akt signaling pathway
Authors: Cheng Lulu , Li Xiaofei , Dong Wenhui , Yang Jing , Li Pengmei , Qiang Xihui , Yin Jiajun , Guo Lianyi
Published: May 2024

Objectives

Gastric cancer (GC) is a prevalent malignant tumor widely distributed globally, exhibiting elevated incidence and fatality rates. The gene LAMC2 encodes the laminin subunit gamma-2 chain and is found specifically in the basement membrane of epithelial cells. Its expression is aberrant in multiple types of malignant tumors. This research elucidated a link between LAMC2 and the clinical characteristics of GC and investigated the potential involvement of LAMC2 in GC proliferation and advancement.

Materials and methods

LAMC2 expressions were detected in GC cell lines and normal gastric epithelial cell lines via qRT-PCR. Silencing and overexpression of the LAMC2 were conducted by lentiviral transfection. A xenograft mouse model was also developed for in vivo analysis. Cell functional assays were conducted to elucidate the involvement of LAMC2 in cell growth, migration, and penetration. Further, immunoblotting was conducted to investigate the impact of LAMC2 on the activation of signal pathways after lentiviral transfection.

Results

In the findings, LAMC2 expression was markedly upregulated in GC cell lines as opposed to normal gastric epithelial cells. In vitro analysis showed that sh-LAMC2 substantially inhibited GC cell growth, migration, and invasion, while oe-LAMC2 displayed a contrasting effect. Xenograft tumor models demonstrated that oe-LAMC2 accelerated tumor growth via high expression of Ki-67. Immunoblotting analysis revealed a substantial decrease in various signaling pathway proteins, PI3K, p-Akt, and Vimentin levels upon LAMC2 knockdown, followed by increased E-cadherin expression. Conversely, its overexpression exhibited contrasting effects. Besides, epithelial-mesenchymal transition (EMT) was accelerated by LAMC2.

Conclusion

This study provides evidence indicating that LAMC2, by stimulating signaling pathways, facilitated EMT and stimulated the progression of GC cells in laboratory settings and mouse models. Research also explored that the abnormal LAMC2 expression acts as a biomarker for GC.
Targeting BMAL1 reverses drug resistance of acute myeloid leukemia cells and promotes ferroptosis through HMGB1-GPX4 signaling pathway
Authors: Zheng Hong , Wu Ting , Lin Zhi , Wang Dan , Zhang Jing , Zeng Ting , Liu Leping , Shen Jie , Zhao Mingyi , Li Jia-Da , Yang Minghua
Published: May 2024

Purpose

Acute myeloid leukemia (AML) is a refractory hematologic malignancy that poses a serious threat to human health. Exploring alternative therapeutic strategies capable of inducing alternative modes of cell death, such as ferroptosis, holds great promise as a viable and effective intervention.

Methods

We analyzed online database data and collected clinical samples to verify the expression and function of BMAL1 in AML. We conducted experiments on AML cell proliferation, cell cycle, ferroptosis, and chemotherapy resistance by overexpressing/knocking down BMAL1 and using assays such as MDA detection and BODIPY 581/591 C11 staining. We validated the transcriptional regulation of HMGB1 by BMAL1 through ChIP assay, luciferase assay, RNA level detection, and western blotting. Finally, we confirmed the results of our cell experiments at the animal level.

Results

BMAL1 up-regulation is an observed phenomenon in AML patients. Furthermore, there existed a strong correlation between elevated levels of BMAL1 expression and inferior prognosis in individuals with AML. We found that knocking down BMAL1 inhibited AML cell growth by blocking the cell cycle. Conversely, overexpressing BMAL1 promoted AML cell proliferation. Moreover, our research results revealed that BMAL1 inhibited ferroptosis in AML cells through BMAL1-HMGB1-GPX4 pathway. Finally, knocking down BMAL1 can enhance the efficacy of certain first-line cancer therapeutic drugs, including venetoclax, dasatinib, and sorafenib.

Conclusion

Our research results suggest that BMAL1 plays a crucial regulatory role in AML cell proliferation, drug resistance, and ferroptosis. BMAL1 could be a potential important therapeutic target for AML.
Effects of concurrent chemoradiotherapy with or without Endostar on the regression of retropharyngeal lymph node and prognosis of patients with locally advanced nasopharyngeal carcinoma: a retrospective study
Authors: Song Jun-Mei , Mo Ning , Lv Yu-Qing , Huang Lu-Lu , Wen Ya-Jing , Liu Ting , Li Zhi-Ru , Wang Ren-Sheng , Zhang Ting-Ting
Published: May 2024

Background and Purpose

To investigate the effect of combining Endostar with concurrent chemoradiotherapy (ECCRT) compared to concurrent chemoradiotherapy (CCRT) on the regression rate of retropharyngeal lymph nodes (RLNs) and the relationship between regression rate of RLNs and prognosis of patients with locally advanced nasopharyngeal carcinoma (LANPC).

Methods

A total of 122 LANPC patients with RLNs metastasis were included. Metastatic RLNs were delineated both before and after treatment slice by slice on the magnetic resonance images cross-section. The regression rate of RLNs, adverse effects (AE) were evaluated. The median regression rate of RLNs was taken as the cut-off value, and the patients were furtherly divided into high regression rate (HRR) group and low regression rate (LRR) group, then survival times were evaluated.

Results

The median regression rates of RLNs in the ECCRT and CCRT groups were 81% and 50%, respectively (P < 0.001). There was no statistically significant difference in the incidence of grade 3/4 AEs between the two groups, except for oral mucositis (ECCRT 26.23% vs. CCRT 44.26%, P = 0.037). The 3-year overall survival (OS), progression-free survival (PFS), distant metastasis-free survival (DMFS) and locoregional failure-free survival (LRFFS) rates in the HRR and LRR groups were 85.48% and 86.67% (P = 0.983), 80.65% and 68.33% (P = 0.037), 83.87% and 85% (P = 0.704), 93.55% and 81.67% (P = 0.033), respectively.

Conclusions

Patients in the ECCRT group had higher regression rates of RLNs and lower incidence of severe oral mucositis. Furthermore, patients in the HRR group had a better 3-year PFS and LRFFS rate than those in the LRR group.
Chromosome-Y haplogroups in Asturias (Northern Spain) and their association with severe COVID-19
Authors: González-Fernández Mar , Vázquez-Coto Daniel , Albaiceta Guillermo M. , Amado-Rodríguez Laura , Clemente Marta G. , Velázquez-Cuervo Lucinda , García-Lago Claudia , Gómez Juan , Coto Eliecer
Published: May 2024

The main objective of this study was to determine whether the common Y-haplogroups were be associated with the risk of developing severe COVID-19 in Spanish male. We studied 479 patients who required hospitalization due to COVID-19 and 285 population controls from the region of Asturias (northern Spain), They were genotyped for several polymorphisms that define the common European Y-haplogroups. We compared the frequencies between patients and controls aged ≤ 65 and >65 years. There were no different haplogroup frequencies between the two age groups of controls. Haplogroup R1b was less common in patients aged ≤65 years. Haplogroup I was more common in the two patient´s groups compared to controls (p = 0.02). Haplogroup R1b was significantly more frequent among hypertensive patients, without difference between the hypertensive and normotensive controls. This suggested that R1b could increase the risk for severe COVID-19 among male with pre-existing hypertension. In conclusion, we described the Y-haplogroup structure among Asturians. We found an increased risk of severe COVID-19 among haplogroup I carriers, and a significantly higher frequency of R1b among hypertensive patients. These results indicate that Y-chromosome variants could serve as markers to define the risk of developing a severe form of COVID-19.
Reversed-phase thin-layer chromatography and ultra-performance liquid chromatography/mass spectrometry to estimate the drug likeness of phosphodiesterase 10A inhibitors with phthalimide core
Authors: Czopek Anna , Żmudzki Paweł , Dąbrowska Monika , Starek Małgorzata , Łątka Kamil , Bajda Marek , Jaromin Anna , Fryc Monika , Zagórska Agnieszka
Published: May 2024

Lipophilicity is a physicochemical parameter well known as a decisive factor for predicting the successful development of a drug. Thus, a balance between potency and physicochemical properties during medicinal chemistry optimization is needed. In this study, the lipophilicity of isoindole-1,3(2H)-dione derivatives designed as phosphodiesterase 10A (PDE10A) inhibitors was determined by chromatographic [reversed-phase thin-layer chromatography (RP-TLC) and ultra-performance liquid chromatography/mass spectrometry (UPLC/MS)] and in silico methods. To assess the correlation between the obtained lipophilicity parameters, principal component analysis (PCA) was performed. logP values obtained by chromatographic (logPRP-TLC and logPUPLC/MS) and in silico methods were compared using the PCA method. The results of PCA revealed that logPUPLC/MS and in silico clogP provided by the ChemDraw program were highly correlated. Compounds’ drug likeness was screened, and the pharmacokinetic properties were predicted. All the investigated compounds displayed drug-likeness properties, and they met the criteria of Lipinski’s rule of five, which predicted the oral bioavailability of drug candidates. Analysis of the influence of physicochemical properties on the biological activity showed that the compounds with increased potency on PDE10A had significantly higher topological polar surface area (TPSA) values. The blood‒brain barrier permeability and the hemolytic activity of model compound 18 were examined. The model compound 18 displayed no toxicity effect on erythrocytes in the hemolytic assay and good parallel artificial membrane permeability. The results showed that phthalimide compounds with benzimidazole moiety are a source of compound-targeted inhibition of PDE10A with balanced physicochemical and drug-likeness properties.
The impact of temperature on vascular function in connection with vascular laser treatment
Authors: Doppegieter M. , van Leeuwen T.G. , Aalders M.C.G. , de Vos J. , van Bavel E.T. , Bakker E.N.T.P
Published: May 2024

Pulsed dye lasers are used effectively in the treatment of psoriasis with long remission time and limited side effects. It is, however, not completely understood which biological processes underlie its favorable outcome. Pulsed dye laser treatment at 585-595 nm targets hemoglobin in the blood, inducing local hyperthermia in surrounding blood vessels and adjacent tissues. While the impact of destructive temperatures on blood vessels has been well studied, the effects of lower temperatures on the function of several cell types within the blood vessel wall and its periphery are not known. The aim of our study is to assess the functionality of isolated blood vessels after exposure to moderate hyperthermia (45 to 60°C) by evaluating the function of endothelial cells, smooth muscle cells, and vascular nerves. We measured blood vessel functionality of rat mesenteric arteries (n=19) by measuring vascular contraction and relaxation before and after heating vessels in a wire myograph. To this end, we elicited vascular contraction by addition of either high potassium solution or the thromboxane analogue U46619 to stimulate smooth muscle cells, and electrical field stimulation (EFS) to stimulate nerves. For measurement of endothelium-dependent relaxation, we used methacholine. Each vessel was exposed to one temperature in the range of 45-60°C for 30 seconds and a relative change in functional response after hyperthermia was determined by comparison with the response per stimulus before heating. Non-linear regression was used to fit our dataset to obtain the temperature needed to reduce blood vessel function by 50% (Half maximal effective temperature, ET50). Our findings demonstrate a substantial decrease in relative functional response for all three cell types following exposure to 55°C-60°C. There was no significant difference between the ET50 values of the different cell types, which was between 55.9°C and 56.9°C (P>0.05). Our data show that blood vessel functionality decreases significantly when exposed to temperatures between 55°C-60°C for 30 seconds. The results show functionality of endothelial cells, smooth muscle cells, and vascular nerves is similarly impaired. These results help to understand the biological effects of hyperthermia and may aid in tailoring laser and light strategies for selective photothermolysis that contribute to disease modification of psoriasis after pulsed dye laser treatment.
Physiological effects of interacting native and invasive bivalves under thermal stress
Authors: Hillebrand Veronika , Dobler Andreas H. , Schwalb Astrid N. , Geist Juergen
Published: May 2024

Across many ecosystems in North America and Europe, native freshwater bivalves (Order Unionida) are threatened by fouling and competition for food by the invasive zebra mussel Dreissena polymorpha. In light of climate change, knowledge on the influence of water temperature on these competitive effects is important, yet poorly understood. This study examines the physiological impact of the interaction between D. polymorpha and the native European unionid Anodonta cygnea over a 28 day—period in response to water temperatures of 12, 19, and 25 °C by comparing their glycogen, glucose, lipid and protein concentrations. The laboratory experiment comprised three treatments: (1) fouling of A. cygnea by D. polymorpha, (2) both species present but not fouling; and (3) a control in which A. cygnea and D. polymorpha were placed separately. Increased water temperatures caused physiological stress in D. polymorpha as evident from reduced glycogen, glucose, lipid and protein concentrations. Dreissena polymorpha benefited from fouling of unionids, as individuals that fouled A. cygnea tended to have increased glycogen, glucose, lipid and protein concentrations. Competitive effects of D. polymorpha over the unionid bivalve species, however, were not intensified by elevated temperatures. Glochidia release, lower infestation intensity, and physiological stress of Dreissena at higher temperatures were likely confounding factors. The results of this study suggest that understanding the physiological consequences of species interactions at changing temperatures can be an important tool to assess future climate change impacts on freshwater bivalves and aquatic community structures.
Contribution of marine macrophytes to pCO2 and DOC variations in human-impacted coastal waters
Authors: Watanabe Kenta , Tokoro Tatsuki , Moki Hirotada , Kuwae Tomohiro
Published: May 2024

Carbon cycles in coastal waters are highly sensitive to human activities and play important roles in global carbon budgets. CO2 sink–source behavior is regulated by spatiotemporal variations in net biological productivity, but the contribution of macrophyte habitats including macroalgae aquaculture to atmospheric CO2 removal has not been well quantified. We investigated the variations in the carbonate system and dissolved organic carbon (DOC) in human-impacted macrophyte habitats and analyzed the biogeochemical drivers for the variations of these processes. Cultivated macroalgal metabolism (photosynthesis, respiration, calcification, and DOC release) was quantified by in situ field-bag experiments. Cultivated macroalgae took up dissolved inorganic carbon (DIC) (16.2–439 mmol-C m−2 day−1) and released DOC (1.2–146 mmol-C m−2 day−1). We estimated that seagrass beds and macroalgae farming contributed 0.8 and 0.4 mmol-C m−2 day−1 of the in situ total CO2 removal (5.7 and 6.7 mmol-C m−2 day−1, respectively) during their growing period in a semi-enclosed embayment but efficient water exchange (i.e., short residence time) in an open coastal area precluded detection of the contribution of macrophyte habitats to the CO2 removal. Although hydrological processes, biological metabolism, and organic carbon storage processes would contribute to the net CO2 sink–source behavior, our analyses distinguished the contribution of macrophytes from other factors. Our findings imply that macroalgae farming, in addition to restoring and creating macrophyte habitats, has potential for atmospheric CO2 removal.
A novel remote sensing-based approach to determine loss of agricultural soils due to soil sealing — a case study in Germany
Authors: Säurich Annelie , Möller Markus , Gerighausen Heike
Published: May 2024

Soils provide habitat, regulation and utilization functions. Therefore, Germany aims to reduce soil sealing to 30 ha day$$^{-1}$$

-
1

by 2030 and to eliminate it by 2050. About 55 ha day$$^{-1}$$

-
1

of soil are damaged (average 2018–2021), but detailed information on its soil quality is lacking. This study proposes a new approach using geo-information and remote sensing data to assess agricultural soil loss in Lower Saxony and Brandenburg. Soil quality is assessed based on erosion resistance, runoff regulation, filter functions, yield potential and the Müncheberg Soil Quality Rating from 2006 to 2015. Data from the German Soil Map at a scale of 1:200,000 (BÜK 200), climate, topography, CORINE Land Cover (CLC) and Imperviousness Layer (IMCC), both provided by the Copernicus Land Monitoring Service (CLMS), are used to generate information on soil functions, potentials and agricultural soil loss due to sealing. For the first time, soil losses under arable land are assessed spatially, quantitatively and qualitatively. An estimate of the qualitative loss of agricultural soil in Germany between 2006 and 2015 is obtained by intersecting the soil evaluation results with the quantitative soil loss according to IMCC. Between 2006 and 2015, about 73,300 ha of land were sealed in Germany, affecting about 37,000 ha of agricultural soils. This corresponds to a sealing rate of 11 ha per day for Germany. In Lower Saxony and Brandenburg, agricultural soils were sealed at a rate of 1.9 ha day$$^{-1}$$

-
1

and 0.8 ha day$$^{-1}$$

-
1

respectively, removing these soils from primary land use. In Lower Saxony, 75% of soils with moderate or better biotic yield potential have been removed from primary land use, while in Brandenburg this figure is as high as 88%. Implementing this approach can help decision-makers reassess sealed land and support Germany’s sustainable development strategy.
The Simons Observatory: Production-Level Fabrication of the Mid- and Ultra-High-Frequency Wafers
Authors: Duff Shannon M. , Austermann Jason , Beall James A. , Daniel David P. , Hubmayr Johannes , Jaehnig Greg C. , Johnson Bradley R. , Jones Dante , Link Michael J. , Lucas Tammy J. , Sonka Rita F. , Staggs Suzanne T. , Ullom Joel , Wang Yuhan
Published: May 2024

The Simons Observatory (SO) is a cosmic microwave background instrumentation suite in the Atacama Desert of Chile. More than 65,000 polarization-sensitive transition-edge sensor (TES) bolometers will be fielded in the frequency range spanning 27 to 280 GHz, with three separate dichroic designs. The mid-frequency 90/150 GHz and ultra-high-frequency 220/280 GHz detector arrays, fabricated at NIST, account for 39 of 49 total detector modules and implement the feedhorn-fed orthomode transducer-coupled TES bolometer architecture. A robust production-level fabrication framework for these detector arrays and the monolithic DC/RF routing wafers has been developed, which includes single device prototyping, process monitoring techniques, in-process metrology, and cryogenic measurements of critical film properties. Application of this framework has resulted in timely delivery of nearly 100 total superconducting focal plane components to SO with $$88\%$$

88
%

of detector wafers meeting nominal criteria for integration into a detector module: a channel yield $$>95\%$$

>
95
%

and $$T_{\textrm{c}}$$

T
c

in the targeted range.
The Relationship Between Strategic Human Resource Management Practices and the Employment of Vulnerable Workers: A Two-Wave Study Among Employers
Authors: Kersten Amber , van Woerkom Marianne , Geuskens Goedele A. , Blonk Roland W. B.
Published: May 2024

Purpose

To improve the inclusion of vulnerable workers in the labor market, employer behavior is key. However, little is known about the effectiveness of strategic Human Resource Management (HRM) practices that employers use to employ vulnerable workers. Therefore, this exploratory study investigates the association between strategic HRM practices (based on social legitimacy, economic rationality and employee well-being) and the actual and intended employment of vulnerable workers in the future.

Methods

In total, 438 organizations included in the Netherlands Employers Work Survey participated in a two-wave study with a nine-month follow-up period. Logistic regression models were used to estimate the relationship between strategic HRM practices (T0) with the employment of vulnerable workers (T1) and intentions to hire vulnerable workers (T1), while controlling for organizational size, sector, and employment of vulnerable workers at baseline.

Results

Employers who applied strategic HRM practices based on social legitimacy (e.g., inclusive mission statement or inclusive recruitment) or economic rationality (e.g., making use of reimbursements, trial placements, or subsidies) at T0 were more likely to employ vulnerable workers and to intend to hire additional vulnerable workers at T1. No significant results were found for practices related to employee well-being.

Conclusion

Since different types of strategic HRM practices contribute to the inclusion of vulnerable workers, employers can build on their strategic priorities and strengths to create inclusive HRM approaches. Future research is needed to study whether these strategic HRM domains also relate to sustainable employment of vulnerable workers.
Topological twists of massive SQCD, Part I
Authors: Aspman Johannes , Furrer Elias , Manschot Jan
Published: May 2024

We consider topological twists of four-dimensional $$\mathcal {N}=2$$

N
=
2

supersymmetric QCD with gauge group SU(2) and $$N_f\le 3$$

N
f

≤
3

fundamental hypermultiplets. The twists are labelled by a choice of background fluxes for the flavour group, which provides an infinite family of topological partition functions. In this Part I, we demonstrate that in the presence of such fluxes the theories can be formulated for arbitrary gauge bundles on a compact four-manifold. Moreover, we consider arbitrary masses for the hypermultiplets, which introduce new intricacies for the evaluation of the low-energy path integral on the Coulomb branch. We develop techniques for the evaluation of these path integrals. In the forthcoming Part II, we will deal with the explicit evaluation.
Growth of Nordic container forest tree seedlings in some peatless and peat-reduced growing media
Authors: Heiskanen Juha , Ruhanen Hanna , Himanen Katri , Kivimäenpää Minna , Silvan Niko
Published: May 2024

Carbon emissions and environmental concerns have led to the aspiration to reduce peat extraction and its use as a growing medium (GM). In Nordic Forest tree seedling production, Sphagnum peat has been almost exclusively used as a GM in seedling containers due to its good properties and availability. This study examined the feasibility of several peat-reduced and peat-free GM in container tree seedling production of the key tree species in Nordic forestry (Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies L. Karst.) and silver birch (Betula pendula Roth) as well as Russian larch (Larix archangelica P. Lawson & C. Lawson ex Trautv.) and common alder (Alnus glutinosa (L.) Gaertn.). Pure or mixed GM, consisting of low-humified Sphagnum peat (pure as a control), harvested Sphagnum moss, wood fiber, cow manure digestate from a biogas plant, and a common reed compost were tested. Seedlings were grown in controlled conditions in greenhouse experiments and also in larger-scale commercial tree nurseries. Peat-reduced media containing peat of at least 50 vol% provided growth that is similar to pure Sphagnum peat for the tested species and container types. All the studied alternative media can yield marketable seedlings, although commonly of reduced size and requiring special adjustments in growing management. The studied media have a potential to reduce or replace peat in seedling production, but adjustments of their physical and chemical properties, as well as of seedling fertigation and management procedures, are required. The economic feasibility and environmental sustainability of these GM, as well as the outplanting success of seedlings grown in these media, remain to be studied.
Does Financial Inclusion Matter to Population Health? Insight From a Global Dataset
Authors: Acheampong Alex O. , Tetteh Godsway Korku
Published: May 2024

Currently, extensive empirical studies exist on the impact of financial inclusion on socio-economic outcomes such as economic growth, inequality, and poverty. However, research on financial inclusion and population health is limited and still at the infant stage. Therefore, this article adds to knowledge by inquiring into the health implications of financial inclusion using a global panel dataset for 121 countries between 2004 and 2020. After accounting for endogeneity with heteroskedasticity-based instrumental variable regression and cross-sectional dependency with the Driscol–Kraay estimator, we documented that financial inclusion improves population health outcomes. We found that the effect of financial inclusion on population health outcomes also depends on the level of information and communication technology penetration and existing socio-economic conditions in a country. Following this, we showed that financial inclusion improves population health outcomes in countries with lower income inequality and higher GDP per capita, mobile and internet penetration. We further found heterogeneity in the results across geographical regions and income groups. Following these findings, we suggest that financial inclusion policies could enhance population health.
Positive impacts of Nannochloropsis oculata supplementation on gene expression of immune and antioxidant markers and metabolic profile of Barki sheep in the transition period and lipogenic effects on progeny
Authors: El-Sayed Ahmed , Ebissy Eman , Ateya Ahmed
Published: May 2024

Nannochloropsis species should be given priority when it comes to microalgae that should be added to feed since they are suitable for intense culture and have a high concentration of PUFAs (especially EPA), antioxidants, and certain vitamins. This study investigated the possible immune and antioxidant impacts of Nannochloropsis supplementation on Barki ewes during transition period and their newly born lambs. Three weeks prior to the expected time of lambing, the researched ewes were divided into two equal groups of thirty ewes each. The second group, on the other hand, was fed the same base diet as the first group plus 10 g of commercially available Nannochloropsis powder per kg of concentrate, given daily to each ewe’s concentrate. Findings revealed that supplementation of ewes with Nannochloropsis significantly up-regulated the expression pattern of immune (NFKB, RANTES, HMGB1, TNF-α, IRF4, TLR7, CLA-DRB3.2, IL1B, IL6, CXCL8, S-LZ, and Cathelicidin), and antioxidant (SOD1, CAT, GPX1, GST, ATOX1, Nrf2 and AhpC/TSA) markers in ewes post-lambing and their newly born lambs. Additionally, mRNA levels of lipogenic (ACACA, FASN SCD, LPL, and BTN1A) markers were significantly up-regulated in lambs from supplemented ewes than control ones. There was a significant increase in the WBCs, Hb, RBc count, serum level of glucose, total protein, triacylglycerol and total cholesterol, GPx, catalase, IL1α and IL6 with significantly decreased serum level of TNF-α and MDA in supplemented ewes after lambing as compared with control ones. There was also a significant increase in WBCs, Hb, RBc count, birth weight and body temperature with significantly decreased in the serum levels of TNF-α and stillbirth of newly born lambs from supplemented ewes as compared to other lambs from control ones.
The Michaelis–Menten Reaction at Low Substrate Concentrations: Pseudo-First-Order Kinetics and Conditions for Timescale Separation
Authors: Eilertsen Justin , Schnell Santiago , Walcher Sebastian
Published: May 2024

We demonstrate that the Michaelis–Menten reaction mechanism can be accurately approximated by a linear system when the initial substrate concentration is low. This leads to pseudo-first-order kinetics, simplifying mathematical calculations and experimental analysis. Our proof utilizes a monotonicity property of the system and Kamke’s comparison theorem. This linear approximation yields a closed-form solution, enabling accurate modeling and estimation of reaction rate constants even without timescale separation. Building on prior work, we establish that the sufficient condition for the validity of this approximation is $$s_0 \ll K$$

s
0

≪
K

, where $$K=k_2/k_1$$

K
=

k
2

/

k
1

is the Van Slyke–Cullen constant. This condition is independent of the initial enzyme concentration. Further, we investigate timescale separation within the linear system, identifying necessary and sufficient conditions and deriving the corresponding reduced one-dimensional equations.
Neuroprotective Role of Selenium Nanoparticles Against Behavioral, Neurobiochemical and Histological Alterations in Rats Subjected to Chronic Restraint Stress
Authors: Elfakharany Sarah A. , Eskaros Samir S. , Azhary Nesrine M. El , Abdelmonsif Doaa A. , Zeitoun Teshreen M. , Ammar Gamal A. G. , Hatem Youssef A.
Published: May 2024

Chronic stress induces changes in the prefrontal cortex and hippocampus. Selenium nanoparticles (SeNPs) showed promising results in several neurological animal models. The implementation of SeNPs in chronic restraint stress (CRS) remains to be elucidated. This study was done to determine the possible protective effects of selenium nanoparticles on behavioral changes and brain oxidative stress markers in a rat model of CRS. 50 rats were divided into three groups; control group (n = 10), untreated CRS group (n = 10) and CRS-SeNPs treated group (n = 30). Restraint stress was performed 6 h./day for 21 days. Rats of CRS-SeNPs treated group received 1, 2.5 or 5 mg/kg SeNPs (10 rats each) by oral gavage for 21 days. Rats were subjected to behavioral assessments and then sacrificed for biochemical and histological analysis of the prefrontal cortex and hippocampus. Prefrontal cortical and hippocampal serotonin levels, oxidative stress markers including malondialdehyde (MDA), reduced glutathione (GSH) and glutathione peroxidase (GPx), tumor necrosis factor alpha (TNF-α) and caspase-3 were assessed. Accordingly, different doses of SeNPs showed variable effectiveness in ameliorating disease parameters, with 2.5 mg/kg dose of SeNPs showing the best improving results in all studied parameters. The present study exhibited the neuroprotective role of SeNPs in rats subjected to CRS and proposed their antioxidant, anti-inflammatory and anti-apoptotic effects as the possible mechanism for increased prefrontal cortical and hippocampal serotonin level, ameliorated anxiety-like and depressive-like behaviors and improved prefrontal cortical and hippocampal histological architecture.
Dimensional and Structural Instability of Electrospun Polylactic Acid Membranes in Liquid Environments: Role of Water, Ethanol, and Temperature
Authors: Hambleton Annaliese Rebecca , Mele Elisa
Published: May 2024

Electrospun nanofibres of polylactic acid (PLA) are suggested for a variety of uses, including scaffolds for tissue engineering, components of drug delivery devices, sustainable packaging materials and membranes for liquid filtration/purification. For all these applications, it is critical to consider the stability of the PLA electrospun materials once in operation. Exposure to certain liquids and temperatures can modify their dimensions, shape, surface topography and mechanical response and compromise their performance. In this study, electrospun PLA mats were exposed to water and ethanol solutions, at different temperatures and for defined time periods, and changes in their properties were analysed. It was found that the impact of water on area shrinkage and fibre arrangement strongly depended on temperature, particularly if the treatment was performed at the glass transition temperature of PLA. Ethanol, instead, induced significant alterations in the size, morphology, and elastic modulus of the electrospun mats, even at room temperature and determined the formation of crimped structures. This work provides insights into the conditions that can critically affect the properties of PLA electrospun fibres and, hence, impact on their usage.
Local Watershed Properties Cannot Explain Divergent Dynamics of Pacific Herring in an Urbanizing Estuary
Authors: Davis Leah R. , Urfer Fabienne , Essington Timothy E. , Feist Blake E. , Francis Tessa B.
Published: May 2024

Pacific herring (Clupea pallasii) is a foundational species in Puget Sound (Washington State, U.S.A.) and is subject to many anthropogenic threats. We assessed the overall status of the Puget Sound Pacific herring sub-stock complex and asked whether watersheds with less urban or agricultural land cover, less impervious surface, and lower human density were associated with better stock status. To this end, we developed multiple metrics of sub-stock population status; characterized watershed properties with respect to land use/land cover, percent impervious surfaces, and human density; and used statistical model selection to evaluate the weight of evidence in support of our hypotheses. Overall, the status of sub-stocks was poor; metrics for most sub-stocks indicate a decline from 1996–2021. However, the status metrics of sub-stocks were not related to recent (2016) watershed characteristics or the rate of change in watershed characteristics from the mid-1990s to 2016. While the cumulative effects of local human land use throughout Puget Sound may be contributing to the deterioration of spawning biomass, these results also suggest that other drivers that operate at larger scales (e.g., predation, disease, climate) are likely important.
Digital contact tracing technology in the COVID-19 pandemic: a systematic review
Authors: Irwin Nicole , Aisyah Dewi Nur , Rahman Fauziah Mauly , Manikam Logan
Published: May 2024

Introduction

The COVID-19 pandemic prompted public health teams across the world to emphasize case identification, contact tracing, and isolation in outbreak management strategies. Contact tracing was advanced by global access to mobile phones to develop and implement digital contact tracing (DCT) technology with the objective of increasing the rate of contact tracing while reducing the resources required.

Purpose

This study aimed to describe the DCT technology used during the COVID-19 pandemic across the world, and to identify differences and similarities between characteristics and uses.

Methods

This review followed the PRISMA (2020) guidelines for systematic reviews. Literature searches were conducted using Embase, MEDLINE, and PubMed and were restricted to English studies published between 2019 to 2023. Studies were excluded if they did not report findings for DCT during COVID-19, did not provide data for technology characteristics or outcomes, or were a study design listed for exclusion.

Results

Sixty one studies were included in the review producing results for 147 digital contact tracing technologies across 83 countries. The majority of digital contact tracing (DCT) technologies are government-owned (75.6%), primarily developed for COVID-19 tracing (96.4%). Bluetooth is the most favored technology (70%) used in their development, followed by GPS (30%) and QR codes (22%). Applications are the preferred platform (90.9%), with a few using applets (6.3%) and wearable devices (1.4%). Only 2 DCT technologies have achieved over 100 million downloads or uses (3.1%). Most DCTs fall into the 1–9.99 million downloads range (27.7%) and 10,000–99,999 downloads (20%). The majority of DCTs are voluntarily used by the population (63.6%), while 27.3% are mandated for use.

Conclusions

Digital contact tracing technologies were developed and implemented globally as a strategy in emergency outbreak management to reduce the spread of disease. This review describes the use of DCT across the world by identifying key features and characteristics that will serve as a lesson learned for improvement of existing DCT technologies for other emergency response outbreak management.
Research on the factors influencing the width of hydraulic fractures through layers
Authors: Liang Yunpei , Wang Mao , Luo Yongjiang , Rui Tao , Wang Xing , Meng Yu
Published: May 2024

The method of segmented hydraulic fracturing in the coal seam roof has proven to be an efficient technique for coalbed methane exploitation. However, the behavior of hydraulic fractures in multilayer formations with significant differences in mechanical properties is still unclear. This paper studied the variation in hydraulic fracture width at the coal-rock interface by employing experimental method with a true triaxial hydraulic fracturing experimental system and numerical simulation method. Results revealed that the hydraulic fracture more likely to expanded along the coal-rock interface instead of break through it with the small horizontal stress difference and low flow rate injection condition. And improving the injection flow rate lager than a critical value, the hydraulic fracture tends to break through the coal-rock interface. Hydraulic fractures in both mudstone and coal beds exhibited a trend of increasing and then decreasing of fracture width at the interface. Since the strength of the coal seam was lower compared to that of the mudstone, maintaining high pressure was no longer necessary when the hydraulic fracture crossed the interface and entered the coal seam, leading to a reduction in fracture width within the mudstone. During the later stages of fracturing, the entry of proppant into the coal seam became challenging, resulting in a phenomenon characterized by excessive fluid but insufficient sand. The time required for the fracture width to traverse the proppant was found to be inversely proportional to the difference in horizontal ground stress and the flow rate of the fracturing fluid. And it was directly proportional to the modulus of elasticity, permeability of the coal seam, and interface strength. The interface strength has the greatest influence on the width of hydraulic fractures. In conclusion, this study provides valuable insights into the behavior of hydraulic fractures in multilayer formations with varying mechanical properties. The findings contribute to a better understanding of the factors affecting hydraulic fracture width within coal seams, which can ultimately enhance the efficiency of coalbed methane exploitation.
Assessment of demand consumptions and potential viability of installation of biogas digester in melani village Eastern Cape, South Africa
Authors: Mthimunye Thabiso Vincent , Mukumba Patrick , Makaka Golden
Published: May 2024

Africa relies heavily on traditional fossil fuel sources, especially coal. To solve the energy problem in rural areas, the country initiated the production and distribution of several renewable energy technologies. Biogas has been proven viable and has begun as a promising technology among several promising technologies. It has been one of the most successful models for producing clean, environmentally friendly, cost-effective energy sources and has multiple benefits. A financial analysis will be conducted, including an estimation of the total investment, the present value of the annual investment, the present value of the investment, and a simple payback period. To determine the demand, onset CTA-A hobo current transducers were installed in three families to measure everyday use in summer and winter. The Data acquisition system has shown that each home consumes an average of 140 kWh of electricity per month. Finally, the biogas digester's analytical life cycle cost analysis shows that the simple payback period would be approximately two years.
Defining Mechanistic Pathways for Anthropogenic Noise Impact on Avian Species
Authors: Engel Margret S. , Young Robert J. , Davies William J. , Waddington David , Wood Michael D.
Published: May 2024

Purpose of Review

This review collates and analyses data on noise exposure of birds in relation to avian hearing system performance. It provides new insights into the mechanistic pathways of anthropogenic noise impact on avian species.

Recent Findings

Noise impacts both humans and wildlife. Birds are of conservation concern, given the recent reports of major global declines in bird populations and that one in eight bird species is threatened with extinction. Studies of noise impacts on birds have been, and continue to be, published. Whilst many of these studies report associations between noise and a response in birds, relatively few provide clear demonstration of the mechanisms of impact.

Anatomical and physiological datasets were compiled for species representing nineteen avian orders. Information on noise sources, propagation path and habitat selection was also collated. Bird order was not a good predictor of bird hearing frequencies, but body dimensions were. In general, smaller birds were found to have higher peak hearing frequencies than larger birds. Cranium height was the strongest predictor of peak hearing frequency for birds. These findings provide mechanistic context to noise impacts on birds and a potential basis for predicting responses of avian species to different noise environments.
Relational Skills and Corporate Productivity in a Comparative Size Class Perspective
Authors: Becchetti Leonardo , Mancini Sara , Solferino Nazaria
Published: May 2024

Drawing on insights from various fields within game theory literature, such as strategic interactions, social dilemmas, gift exchange, and procedural utility, we argue that corporate social responsibility (CSR) and relational skills—whether between firms, employers and workers, among workers themselves, or with stakeholders—are associated with positive effects on productivity. We test this hypothesis using a comparative approach across small, medium, and large Italian firms, leveraging data from the 2019 CSR survey conducted by the Italian Statistical Institute (ISTAT). Our analysis reveals that firm size plays a crucial role in the impact of relational skills on added value per worker, even after controlling for relevant factors. Key components of relational skills identified in our study include corporate policies focusing on workers' well-being, prioritizing teamwork attitudes in recruitment, supporting initiatives within the local productive network, and involving stakeholders in CSR initiatives. Our findings indicate that stakeholder engagement positively impacts all firm sizes, while worker well-being is particularly significant for small and medium firms, local network initiatives for medium and large firms, and teamwork for medium-sized firms. Instrumental variable estimates find evidence of a causal link beyond these correlations. We conclude that firm size exhibits an inverse U-shaped effect on the impact of teamwork skills, reduces the influence of gift exchange mechanisms, and strengthens the effects of investments in the local productive environment on added value per worker.
Homology and Euler characteristic of generalized anchored configuration spaces of graphs
Authors: Kozlov Dmitry N.
Published: May 2024

In this paper we consider the generalized anchored configuration spaces on n labeled points on a graph. These are the spaces of all configurations of n points on a fixed graph G, subject to the condition that at least q vertices in some pre-determined set K of vertices of G are included in each configuration. We give a non-alternating formula for the Euler characteristic of such spaces for arbitrary connected graphs, which are not trees. Furthermore, we completely determine the homology groups of the generalized anchored configuration spaces of n points on a circle graph.
The improved residual power series method for the solutions of higher-order linear and nonlinear boundary value problems
Authors: Gillani Rimshah , Dawar Abdullah , Khan Hamid
Published: May 2024

IRPSM is the extended form of RPSM that gives an approximate solution to boundary value problems without requiring an exact solution. This method creates a truncated series for the determination of the missing initial conditions. The present study is conducted to evaluate semi-numerical solutions to differential equations using the Improved Residual Power Series Method (IRPSM). Our main objective is to check whether the proposed scheme is efficient for solving such equations or not. The solution of differential equations has been approximated using truncated residual power series. The method is used to solve differential equations for higher-order linear and non-linear boundary value problems. Absolute errors for the solved problems are calculated to ensure accuracy. It is also compared with some known methods, like the Differential Transform Method (DTM) and the Homotopy Perturbation Method (HPM). For the calculations, Mathematica 12.0 software is used. The computed results are compared to the exact solutions as well as the available results in the literature. The results showed that the results obtained by IRPSM are closely related to the exact solution when compared to the DTM and HPM results.