Функціональний стан прооксидантної та антиоксидантної систем у легенях при екcпериментальному алергічному альвеоліті за умов іммобілізаційного стресу і корекція його порушень корвітином
Authors: Regeda M. Ye. , Kovalska M. Ye. 
Published: May 2018

Introduction. Experimental allergic alveolitis (EAA) takes an important part among the diseases of the allergic genesis of the bronchopulmonary unit. The sharp deterioration of the socioeconomic situation in Ukraine, the high psycho-emotional stress in society, the long-term systematic impact of adverse factors are often combined, causing a stressful situation in the body.The aim of the study – to learn the functional state of the prooxidant and antioxidant system in the lungs of the mollusks under the conditions of the development of experimental immobilization stress and experimental allergic alveolitis and their correction with corvethene.Research Methods. The object – 80 guinea pigs (females) divided into 8 experimental groups, with 10 animals in each. 1st group – intact animals; 2, 3, 4 groups – the experimental model of allergic alveolitis (1st, 2nd, 34th day)to the treatment of K.5, 6, 7 groups – EAA under conditions of experimental immobilization stress (EIS ) (1st, 2nd, 34th day) to C treatment. Group 8 – EAA in the conditions of EIS (34th day) after treatment C, which for 10 days was administered intravaginal drug "Corvitin" (Borshchahivskyi Chemical and Pharmaceutical factory) in dose of 40 mg/kg. The animals then decapitated and determined in the lungs the content of the LPO products and the activity of the enzymes AOC. The content of diene conjugates (DK) – method of V. H. Havrylov, V. I. Mishkorudna, malone dialdehyde (MDA) – E. N. Korobeinikov, superoxide dismutase activity (SOD) – by R. Fried method, catalase activity (CT) – by B. Holmes, C. Masters. Immobilization stress – by the method of PID Horizon [10]. Experimental AA – by the method of O. Orekhov, Yu. A. Kyrilov. Statistical processing of digital data – Styuden's method. Statistically reliable results were considered, for which p≤0.05.Results and Discussion. During experimental studies, there was a change in the activity of antioxidant protection and lipid peroxidation in the dynamics of EAA development. There are changes in the equilibrium between the prooxidant and antioxidant systems in the direction of the growth of prooxidant and inhibition of antioxidant systems. According to the results, there is a significant activation of antioxidant mechanisms before the first day, with a gradual exhaustion up to 34 days of the disease and an increase in the processes of lipoperoxidation up to 34 days. Under conditions of combined pathological processes – experimental AA and immobilization stress, there is a significant stimulation of the antioxidant and prooxidant antioxidant systems. After treatment for ten days, mullahs with experimental AA in the conditions of EIS and treated K, which was administered at a dose of 40 mg/kg. there is a significant inhibition of LPO, and an increase in the activity of the antioxidant system – superoxide dismutase.Conclusions. The obtained results of the study showed the failure of the antioxidant system to dispose of the products of the LPA in the experimental AA under stress and its exhaustion in early periods of the disease. With theuse of corvetone, which has membrane-stabilizing, immune-regulating, anti-inflammatory and anti-ischemic effects, there was a decrease in the damaging effect of LPO and a significant increase in antioxidant defense, which in turn indicates the corrective effect of corvetane on the parameters of prooxidant and antioxidant systems and provides an opportunity for further study and experimental researches.