bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Life Evidence of Mepolizumab Treatment in Chronic Rhinosinusitis with Nasal Polyps: A Multicentric Study
Authors: Cavaliere Carlo , Loperfido Antonella , Ciofalo Andrea , Di Michele Loreta , Begvarfaj Elona , Bellocchi Gianluca , Bugani Marcella , de Vincentiis Marco , Greco Antonio , Millarelli Stefano , Plath Michaela , Sculco Eleonora , Masieri Simonetta
Published: June 2024

Background: The introduction of biological drugs in the management of chronic rhinosinusitis with nasal polyps (CRSwNP) is allowing new and increasingly promising therapeutic options. This manuscript aims to provide a multicenter trial in a real-life setting on Mepolizumab treatment for severe uncontrolled CRSwNP with or without comorbid asthma. Methods: A retrospective data analysis was jointly conducted at the Otolaryngology–Head and Neck Surgery departments of La Sapienza University and San Camillo Forlanini Hospital in Rome. Both institutions participated by sharing clinical information on patients with CRSwNP treated with Mepolizumab. Patients were evaluated before starting Mepolizumab, at six months and at twelve months from the first drug administration. During follow–up visits, patients underwent endoscopic evaluation, quality of life assessment, nasal symptoms assessment, and blood tests to monitor mainly neutrophils, basophils, eosinophils, and IgG, IgA, and IgE assay. Results: Twenty patients affected by CRSwNP and treated with Mepolizumab were enrolled (12 females and 8 males with a mean age of 63.7 years). Sixteen patients (80%) had concomitant asthma. During follow-up, a gradual improvement in nasal polyp score, quality of life and nasal symptoms, assessed by SNOT-22 and VAS and loss of smell measured by olfactory VAS, was found. Regarding blood tests, eosinophils decreased gradually, while other blood parameters showed no statistically significant changes. Conclusions: Mepolizumab has been shown to be effective in the therapeutic management of patients with CRSwNP. Further studies are needed to support our findings and better understand the underlying immune pathways to predict patients’ response to biological treatment in CRSwNP.
Assessment of the Retinal Ganglion Cell Layer after Uncomplicated Cataract Surgery
Authors: Abou-Jokh Rajab Bassam , Doncel-Fernández Carlos , Sánchez-Liñan Noelia , Castro-Luna Gracia
Published: June 2024

(1) Background: This research aimed to evaluate the changes in ganglion cell layer thickness (GCLT) after uncomplicated cataract surgery in patients without previous ocular pathology and the impact of the appearance of cystoid macular edema on the GCLT and visual acuity. (2) Methods: The evaluation of 174 patients was performed with the indication of uncomplicated cataract surgery. The variables analyzed were demographic data, best-corrected visual acuity (BCVA), cataract type and OCT (Optical Coherence Tomography) measurements of central macular thickness (CMT), and the presence of cysts and GCLT preoperatively and one day, one and three months after surgery. (3) Results: There was a relationship between the postoperative increase in retinal GCLT and BCVA after uncomplicated cataract surgery. The presence of microcysts reduced the thickness of the GCL, which is significantly related to the loss of BCVA. The appearance of cystoid macular edema one month after surgery was also related to the preoperative CMT. There was a statistically significant decrease in preoperative GCL but a statistically significant increase in preoperative CMT in patients with microcysts one-month post-surgery. (4) Conclusions: There is a relationship between postoperative retinal GCLT and BCVA after uncomplicated cataract surgery. The presence of microcysts significantly reduces the thickness of the GCL, which is significantly related to the loss of BCVA.
Comparison of Intense Pulsed Light Treatments including Upper Lid or Lateral Canthus in Patients of Meibomian Gland Dysfunction
Authors: Min Ji Sang , Jun Ikhyun , Kim Tae-im , Arita Reiko , Seo Kyoung Yul
Published: June 2024

Background: To determine the differences in the effects of intense pulsed light (IPL) treatment when including the upper and lower lid or lateral canthus area in patients with meibomian gland dysfunction (MGD). Methods: Patients who underwent three IPL treatment sessions at 3-week intervals were divided into three groups according to the treatment sites: group A, lower lid; group B, upper and lower lids; and group C, lower lid and lateral canthal area. Before and after the IPL treatment sessions, we obtained the lid abnormality score (LAS), meibum expressibility (ME), meibum quality (MQ), lipid layer thickness (LLT), type I Schirmer test (ST), tear break-up time (TBUT) test, corneal fluorescein staining scores (CFSs), and Ocular Surface Disease Index (OSDI). Results: IPL treatment significantly improved LASs, ME, MQ, TBUT, CFS, and OSDI values in all groups. Differences in LAS values before and after IPL treatment were significantly greater in groups B and C than those in group A. Conclusions: IPL treatment encompassing the upper lid and lateral canthus together with the lower lid elicited additional improvement in patients with MGD. The additional effect on treating the lateral canthus was similar to the effect observed on the additional treatment of the upper lid.
Surgical Outcomes of Hugo™ RAS Robot-Assisted Partial Nephrectomy for Cystic Renal Masses: Technique and Initial Experience
Authors: Prata Francesco , Iannuzzi Andrea , Tedesco Francesco , Ragusa Alberto , Civitella Angelo , Pira Matteo , Fantozzi Marco , Sica Leonilde , Scarpa Roberto Mario , Papalia Rocco
Published: June 2024

Background: The Hugo™ Robot-Assisted Surgery (RAS) system is a new cutting-edge robotic platform designed for clinical applications. Nevertheless, its application for cystic renal tumors has not yet been thoroughly investigated. In this context, we present an initial series of Robot-Assisted Partial Nephrectomy (RAPN) procedures carried out using the Hugo™ RAS system for cystic renal masses. Methods: Between October 2022 and January 2024, twenty-seven RAPN procedures for renal tumors were performed at Fondazione Policlinico Universitario Campus Bio-Medico. Our prospective board-approved dataset was queried for “cystic features” (n = 12). Perioperative data were collected. The eGFR was calculated according to the CKD-EPI formula. Post-operative complications were reported according to the Clavien–Dindo classification. Computed tomography (CT) scans for follow-up were performed according to the EAU guidelines. Trifecta was defined as the coexistence of negative surgical margin status, no Clavien–Dindo grade ≥ 3 complications, and eGFR decline ≤ 30%. Results: All the patients successfully underwent RAPN without the need for conversion or additional port placement. The median docking and console time were 5.5 (IQR, 4–6) and 79.5 min (IQR, 58–91 min), respectively. No intraoperative complications occurred, as well as clashes between instruments or with the bedside assistant. Two minor postoperative complications were recorded (Clavien–Dindo II). At discharge, serum creatinine and eGFR were comparable to preoperative values. Only one patient (8.4%) displayed positive surgical margins. The rate of trifecta achievement was 91.7%. Conclusions: RAPN for cystic renal masses using the novel Hugo™ RAS system can be safely and effectively performed. This robotic system provided satisfactory peri-operative outcomes, preserving renal function and displaying low postoperative complications and a high trifecta rate achievement.
Wound Dressing Based on Cassava Silk-Chitosan
Authors: Chen Yumei , Lin Haitao , Yue Xinxia , Lai Enping , Huang Jiwei , Zhao Ziyu
Published: June 2024

The application prospects of composite sponges with antibacterial and drug-carrying functions in the field of medical tissue engineering are extensive. A solution of cassava silk fibroin (CSF) was prepared with Ca(NO3)2 as a solvent, which was then combined with chitosan (CS) to create a sponge-porous material by freeze-drying. The CSF-CS composite sponge with a mesh structure was successfully fabricated through hydrogen bonding. Scanning electron microscopy (SEM), Fourier transform infrared absorption (FTIR) and X-ray diffraction (XRD) were employed to investigate the appearance and structure of the cassava silk’s fibroin materials, specifically examining the impact of different mass percentages of CS on the sponge’s structure. The swelling rate and mechanical properties of the CSF-CS sponge were analyzed, along with its antibacterial properties. Furthermore, by incorporating ibuprofen as a model drug into these loaded sponges, their potential efficacy as efficient drug delivery systems was demonstrated. The results indicate that the CSF-CS sponge possesses a three-dimensional porous structure with over 70% porosity and an expansion rate exceeding 400% while also exhibiting good resistance against pressure. Moreover, it exhibits excellent drug-carrying ability and exerts significant bacteriostatic effects on Escherichia coli. Overall, these findings support considering the CSF-CS composite sponge as a viable candidate for use in drug delivery systems or wound dressings.
Polyethylene Terephthalate (PET) Recycled by Catalytic Glycolysis: A Bridge toward Circular Economy Principles
Authors: Enache Andra-Cristina , Grecu Ionela , Samoila Petrisor
Published: June 2024

Plastic pollution has escalated into a critical global issue, with production soaring from 2 million metric tons in 1950 to 400.3 million metric tons in 2022. The packaging industry alone accounts for nearly 44% of this production, predominantly utilizing polyethylene terephthalate (PET). Alarmingly, over 90% of the approximately 1 million PET bottles sold every minute end up in landfills or oceans, where they can persist for centuries. This highlights the urgent need for sustainable management and recycling solutions to mitigate the environmental impact of PET waste. To better understand PET’s behavior and promote its management within a circular economy, we examined its chemical and physical properties, current strategies in the circular economy, and the most effective recycling methods available today. Advancing PET management within a circular economy framework by closing industrial loops has demonstrated benefits such as reduced landfill waste, minimized energy consumption, and conserved raw resources. To this end, we identified and examined various strategies based on R-imperatives (ranging from 3R to 10R), focusing on the latest approaches aimed at significantly reducing PET waste by 2040. Additionally, a comparison of PET recycling methods (including primary, secondary, tertiary, and quaternary recycling, along with the concepts of “zero-order” and biological recycling techniques) was envisaged. Particular attention was paid to the heterogeneous catalytic glycolysis, which stands out for its rapid reaction time (20–60 min), high monomer yields (>90%), ease of catalyst recovery and reuse, lower costs, and enhanced durability. Accordingly, the use of highly efficient oxide-based catalysts for PET glycolytic degradation is underscored as a promising solution for large-scale industrial applications.
Thermal Stability of Encapsulated Carbon-Based Multiporous-Layered-Electrode Perovskite Solar Cells Extended to Over 5000 h at 85 °C
Authors: Tsuji Ryuki , Nagano Yuuma , Oishi Kota , Kobayashi Eiji , Ito Seigo
Published: June 2024

The key to the practical application of organometal–halide crystals perovskite solar cells (PSCs) is to achieve thermal stability through robust encapsulation. This paper presents a method to significantly extend the thermal stability lifetime of perovskite solar cells to over 5000 h at 85 °C by demonstrating an optimal combination of encapsulation methods and perovskite composition for carbon-based multiporous-layered-electrode (MPLE)-PSCs. We fabricated four types of MPLE-PSCs using two encapsulation structures (over- and side-sealing with thermoplastic resin films) and two perovskite compositions ((5-AVA)x(methylammonium (MA))1−xPbI3 and (formamidinium (FA))0.9Cs0.1PbI3), and analyzed the 85 °C thermal stability followed by the ISOS-D-2 protocol. Without encapsulation, FA0.9Cs0.1PbI3 exhibited higher thermal stability than (5-AVA)x(MA)1−xPbI3. However, encapsulation reversed the phenomenon (that of (5-AVA)x(MA)1−xPbI3 became stronger). The combination of the (5-AVA)x(MA)1−xPbI3 perovskite absorber and over-sealing encapsulation effectively suppressed the thermal degradation, resulting in a PCE value of 91.2% of the initial value after 5072 h. On the other hand, another combination (side-sealing on (5-AVA)x(MA)1−xPbI3 and over- and side-sealing on FA0.9Cs0.1PbI3) resulted in decreased stability. The FACs-based perovskite was decomposed from these degradation mechanisms by the condensation reaction between FA and carbon. For side-sealing, the space between the cell and the encapsulant was estimated to contain approximately 1,260,000 times more H2O than in over-sealing, which catalyzed the degradation of the perovskite crystals. Our results demonstrate that MA-based PSCs, which are generally considered to be thermally sensitive, can significantly extend their thermal stability after proper encapsulation. Therefore, we emphasize that finding the appropriate combination of encapsulation technique and perovskite composition is quite important to achieve further device stability.
Effect of Wet Grinding Concrete Slurry Waste on Hydration and Hardening Properties of Cement: Micro-Nano-Scale Modification
Authors: Liu Guishan , Sun Hao , Huang Yongbo , Du Peng
Published: June 2024

The concrete slurry waste (CSW) produced by concrete mixing plants is a type of hazardous waste that is difficult to handle. To better recycle the CSW separated from the aggregates, this study uses a variety of wet-grinding processes to refine the solid in it, replaces some of the cement with the solid particles in wet grinding concrete slurry waste (WCSW), and investigates the properties of WCSW and its effect on the hydration and hardening properties of cement. The results show that a suitable wet-grinding process can ensure that the particle size in WCSW is less than 10 μm, the particle morphology is more flat, and the degree of hydration is higher. The WCSW particles can promote early cement hydration; after adding WCSW, the heat release peak of cement hydration appears earlier and more early hydration products are produced, and with the increase in the substitution amount, the promoting effect on early cement hydration will be more significant. The WCSW particles have a great effect on improving the strength of mortar, especially in the early stage. At 1 d, when the substitution amount is 7.5 wt.%, the compressive and flexural strength is increased by 43.67% and 45.04%; this is related to the filling of matrix pores and the improvement of the interface transition zone by micro- and nanoparticles. After the wet grinding of CSW, fine WCSW particles are obtained, which can improve the performance of cement-based materials by replacing cement.
A Finite Element Model for Simulating Stress Responses of Permeable Road Pavement
Authors: Siao Jhu-Han , Chang Tung-Chiung , Wang Yu-Min
Published: June 2024

Permeable road pavements, due to their open-graded design, suffer from low structural strength, restricting their use in areas with light traffic volume and low bearing capacity. To expand application of permeable road pavements, accurate simulation of stress parameters used in pavement design is essential. A 3D finite element (3D FE) model was developed using ABAQUS/CAE 2021 to simulate pavement stress responses. Utilizing a 53 cm thick permeable road pavement and a 315/80 R22.5 wheel as prototypes, the model was calibrated and validated, with its accuracy confirmed through t-test statistical analysis. Simulations of wheel speeds at 11, 15, and 22 m/s revealed significant impact on pavement depths of 3 cm and 8 cm, while minimal effects were observed at depths of 13 cm and 33 cm. Notably, stress values at a depth of 3 cm with 15 m/s speed in the open-graded asphalt concrete (OGFC) surface layer exceeded those at the speed of 11 m/s, while at a depth of 8 cm in the porous asphalt concrete (PAC) base layer, an opposite performance was observed. This may be attributed to the higher elastic modulus of the OGFC surface layer, which results in different response trends to velocity changes. Overall, lower speeds increase stress responses and prolong action times for both layers, negatively affecting pavement performance. Increasing the moduli of layers is recommended for new permeable road pavements for low-speed traffic. Furthermore, considering the effects of heavy loads and changes in wheel speed, the recommended design depth for permeable road pavement is 30 cm. These conclusions provide a reference for the design of permeable road pavements to address climate change and improve performance.
Numerical Simulation of Friction Stir Welding of Dissimilar Al/Mg Alloys Using Coupled Level Set and Volume of Fluid Method
Authors: Zhang Guanlan , Gao Jinqiang , Wu Chuansong
Published: June 2024

The coupled level set and volume of fluid (CLSVOF) method is proposed to simulate the material distribution and physical properties during dissimilar aluminum/magnesium friction stir welding (FSW) process more accurately. Combined with a computational fluid dynamics model, the FSW process is numerically simulated and the heat transfer and material flow are analyzed. The results show that heat transfer and material flow have great influence on the Al/Mg bonding. In order to verify the accuracy of the model, the calculated results based on different methods are compared with the experimental results, and the Al/Mg interface simulated by the CLSVOF method is in better agreement with the experimental results. Finally, the material distribution and interface evolution near the tool at different times were studied based on the CLSVOF method.
Nicotinamide N-Methyltransferase (NNMT): A New Hope for Treating Aging and Age-Related Conditions
Authors: Li Jing-Jing , Sun Wei-Dong , Zhu Xiao-Juan , Mei Ya-Zhong , Li Wen-Song , Li Jiang-Hua
Published: June 2024

The complex process of aging leads to a gradual deterioration in the function of cells, tissues, and the entire organism, thereby increasing the risk of disease and death. Nicotinamide N-methyltransferase (NNMT) has attracted attention as a potential target for combating aging and its related pathologies. Studies have shown that NNMT activity increases over time, which is closely associated with the onset and progression of age-related diseases. NNMT uses S-adenosylmethionine (SAM) as a methyl donor to facilitate the methylation of nicotinamide (NAM), converting NAM into S-adenosyl-L-homocysteine (SAH) and methylnicotinamide (MNA). This enzymatic action depletes NAM, a precursor of nicotinamide adenine dinucleotide (NAD+), and generates SAH, a precursor of homocysteine (Hcy). The reduction in the NAD+ levels and the increase in the Hcy levels are considered important factors in the aging process and age-related diseases. The efficacy of RNA interference (RNAi) therapies and small-molecule inhibitors targeting NNMT demonstrates the potential of NNMT as a therapeutic target. Despite these advances, the exact mechanisms by which NNMT influences aging and age-related diseases remain unclear, and there is a lack of clinical trials involving NNMT inhibitors and RNAi drugs. Therefore, more in-depth research is needed to elucidate the precise functions of NNMT in aging and promote the development of targeted pharmaceutical interventions. This paper aims to explore the specific role of NNMT in aging, and to evaluate its potential as a therapeutic target.
Effect of Al Substitution on Visible Short-Wave Infrared Reflectance Spectroscopy (VSWIR) of Goethite and Ferrihydrite
Authors: Chen Keyan , Qin Xiaorong , Zhou Jingwen , Tan Wei , Liang Xiaoliang , He Hongping , Zhu Jianxi , Han Mengqi , Luo Lianying
Published: June 2024

Goethite and ferrihydrite are the two major iron hydroxides, essential mineral constituents in the terrestrial surface system. Aluminum (Al) is the most common substituent in iron hydroxides, and it may significantly change the bulk and surficial physicochemical properties of iron hydroxides. Consequently, a practical and convenient approach is needed to efficiently identify the Al substitution degrees of iron hydroxides in natural occurrences. This study presents a comprehensive investigation of the VSWIR characteristics of laboratory-synthesized Al-substituted goethite and ferrihydrite, to establish diagnostic VSWIR parameters for the identification and quantification of Al substitution levels in iron hydroxides. The findings revealed that Al substitution can affect the band positions (P) of goethite and ferrihydrite at ~650 nm, ~900 nm, and ~1400 nm. The relationships between the Al substitution of ferrihydrite and VSWIR parameters can be expressed as P900 = −0.43 × Al(%) + 931 and P1400 = −0.07 × Al(%) + 1428, while that of goethite can be expressed as P650 = 0.42 × Al(%) + 657 and P900 = 2.29 × Al(%) + 936. The peak fitting results showed that the absorption intensity at 480–550 nm linearly decreases with increased Al substitution. The obtained VSWIR spectra of Al-substituted goethite and ferrihydrite provide a critical supplement to the spectral library for (Al) iron hydroxides, and these VSWIR parameters can be utilized for the semi-quantitative determination of Al substitution in natural iron hydroxides
Degradation and Recondensation of Metal Oxide Nanoparticles in Laminar Premixed Flames
Authors: May Nadine , Baumann Werner , Hauser Manuela , Yin Zhiyao , Geigle Klaus Peter , Stapf Dieter
Published: June 2024

The behavior of technical nanoparticles at high temperatures was measured systematically to detect morphology changes under conditions relevant to the thermal treatment of end-of-life products containing engineered nanomaterials. The focus of this paper is on laboratory experiments, where we used a Bunsen-type burner to add titania and ceria particles to a laminar premixed flame. To evaluate the influence of temperature on particle size distributions, we used SMPS, ELPI and TEM analyses. To measure the temperature profile of the flame, we used coherent anti-Stokes Raman spectroscopy (CARS). The comprehensible data records show high temperatures by measurement and equilibrium calculation for different stoichiometries and argon admixtures. With this, we show that all technical metal oxide nanoparticle agglomerates investigated reform in flames at high temperatures. The originally large agglomerates of titania and ceria build very small nanoparticles (<10 nm/“peak 2”) at starting temperatures of <2200 K and <1475 K, respectively (ceria: Tmelt = 2773 K, Tboil = 3873 K/titania: Tmelt = 2116 K, Tboil = 3245 K). Since the maximum flame temperatures are below the evaporation temperature of titania and ceria, enhanced vaporization of titania and ceria in the chemically reacting flame is assumed.
(Sub-)Picosecond Surface Correlations of Femtosecond Laser Excited Al-Coated Multilayers Observed by Grazing-Incidence X-ray Scattering
Authors: Randolph Lisa , Banjafar Mohammadreza , Yabuuchi Toshinori , Baehtz Carsten , Bussmann Michael , Dover Nicholas P. , Huang Lingen , Inubushi Yuichi , Jakob Gerhard , Kläui Mathias , Ksenzov Dmitriy , Makita Mikako , Miyanishi Kohei , Nishiuchi Mamiko , Öztürk Özgül , Paulus Michael , Pelka Alexander , Preston Thomas R. , Schwinkendorf Jan-Patrick , Sueda Keiichi , Togashi Tadashi , Cowan Thomas E. , Kluge Thomas , Gutt Christian , Nakatsutsumi Motoaki
Published: June 2024

Femtosecond high-intensity laser pulses at intensities surpassing 1014 W/cm2 can generate a diverse range of functional surface nanostructures. Achieving precise control over the production of these functional structures necessitates a thorough understanding of the surface morphology dynamics with nanometer-scale spatial resolution and picosecond-scale temporal resolution. In this study, we show that single XFEL pulses can elucidate structural changes on surfaces induced by laser-generated plasmas using grazing-incidence small-angle X-ray scattering (GISAXS). Using aluminium-coated multilayer samples we distinguish between sub-picosecond (ps) surface morphology dynamics and subsequent multi-ps subsurface density dynamics with nanometer-depth sensitivity. The observed subsurface density dynamics serve to validate advanced simulation models representing matter under extreme conditions. Our findings promise to open new avenues for laser material-nanoprocessing and high-energy-density science.
Genetic Diversity of Rhipicephalus (Boophilus) microplus for a Global Scenario: A Comprehensive Review
Authors: Sankar Muthu , Kumar Binod , Manjunathachar Haranahally Vasanthachar , Parthasarathi Balasamudram Chandrasekhar , Nandi Abhijit , Neethu Chemmangat Kunnath Subramanian , Nagar Gaurav , Ghosh Srikant
Published: June 2024

Rhipicephalus microplus poses a substantial threat to livestock health and agricultural economies worldwide. Its remarkable adaptability to diverse environments and hosts is a testament to its extensive genetic diversity. This review delves into the genetic diversity of R. microplus, employing three pivotal genetic markers: the cytochrome c oxidase I (COX1) gene, ribosomal genes, and microsatellites. The COX1 gene, a crucial tool for genetic characterization and phylogenetic clustering, provides insights into the adaptability of ticks. Ribosomal genes, such as internal transcribed spacer regions (ITS-1 and2) as well as 18S and 28S, are routinely utilized for species differentiation. However, their use is limited due to indels (insertions and deletions). Microsatellites and minisatellites, known for their high polymorphism, have been successfully employed to study populations and genetic diversity across various tick species. Despite their effectiveness, challenges such as null alleles and marker variations warrant careful consideration. Bm86, a well-studied vaccine candidate, exhibits substantial genetic diversity. This diversity directly influences vaccine efficacy, posing challenges for developing a universally effective Bm86-based vaccine. Moreover, the review emphasizes the prevalence of genes associated with synthetic pyrethroid resistance. Identifying single nucleotide polymorphisms in the acaricide-resistant genes of R. microplus has facilitated the development of molecular markers for detecting and monitoring resistance against synthetic pyrethroids. However, mutations in sodium channels, the target site for synthetic pyrethroid, correlate well with the resistance status of R. microplus, which is not the case with other acaricide target genes. This study underscores the importance of understanding genetic diversity in developing effective tick management strategies. The choice of genetic marker should be tailored based on the level of taxonomic resolution and the group of ticks under investigation. A holistic approach combining multiple markers and integrating additional molecular and morphological data may offer a more comprehensive understanding of tick diversity and relationships. This research has far-reaching implications in formulating breeding programs and the development of vaccine against ticks and tick-borne diseases (TTBDs) as well as strategies for the management of resistant ticks.
Evaluation of RNA Secondary Stem-Loop Structures in the UTRs of Mouse Hepatitis Virus as New Therapeutic Targets
Authors: Kang Gyuhyun , Lee Sun Hee , Cho Miyeon , Kim Ji-hyeon , Cho Hyosun , Kang Hyojeung
Published: June 2024

MHV-A59 is a beta-coronavirus that causes demyelinating encephalitis and hepatitis in mice. Recently, the mouse infection model of MHV-A59 has been used as an alternative animal infection model for SARS-CoV and SARS-CoV-2, aiding the development of new antiviral drugs. In this study, the MHV-A59 model was employed to investigate the potential of SARS-CoV-2 UTRs as new targets for antiviral drugs. Optimal targets within the MHV-A59 UTRs were identified using a shRNA and siRNA design tool, focusing on RNA secondary stem-loop (SL) structures in the UTRs. We then examined whether the designed RNAi constructs could inhibit MHV-A59 replication. In the 5′UTR, the stem-loop 1 (SL1) was identified as the most effective target, while in the 3′UTR, the minimal element for the initiation of negative-strand RNA synthesis (MIN) proved to be the most effective. Importantly, siRNAs targeting SL1 and MIN structures significantly reduced total RNA synthesis, negative-strand genomic RNA synthesis, subgenomic (sg) RNA synthesis, viral titer, and the plaque size of MHV-A59 compared to the control. Although not statistically significant, the combination of siSL1 and siMIN had a stronger effect on inhibiting MHV-A59 replication than either siRNA monotherapy. Interestingly, while the SL1 structure is present in both MHV and SARS-CoV-2, the MIN structure is unique to MHV. Thus, the SL1 of SARS-CoV-2 may represent a novel and promising target for RNAi-based antiviral drugs.
The Response and Evaluation of Morphology, Physiology, and Biochemistry Traits in Triploid Passiflora edulis Sims ‘Mantianxing’ to Drought Stress
Authors: Su Xin , Yang Zhenxin , Zhou Chiyu , Geng Shili , Chen Shi , Cai Nianhui , Tang Junrong , Chen Lin , Xu Yulan
Published: June 2024

As one of the most influential environmental factors, drought stress greatly impacts the development and production of plants. Triploid-induced Passiflora edulis Sims ‘Mantianxing’ is an important new cultivar for multi-resistance variety selective breeding, which is one of the P. edulis breeding essential targets. However, the performance of triploid ‘Mantianxing’ under drought stress is unknown. In order to study the drought resistance of triploid ‘Mantianxing’, our study compared drought-related indicators in diploids and triploids under natural drought experiments, including morphological, physiological, and biochemical characteristics. Results showed that triploid P. edulis ‘Mantianxing’ showed variable responses to drought treatment. Compared with diploids, triploids showed higher photosynthesis and chlorophyll fluorescence, osmotic adjustment substances, and antioxidant enzyme activity under drought stress and faster chlorophyll biosynthesis and growth recovery after rewatering. Generally speaking, these results indicate that the drought resistance of triploid P. edulis is superior to diploid. This study provides scientific information for breeding stress tolerance variety of P. edulis ‘Mantianxing’ new cultivar.
Recruitment and Aggregation Capacity of Tea Trees to Rhizosphere Soil Characteristic Bacteria Affects the Quality of Tea Leaves
Authors: Jia Xiaoli , Lin Shaoxiong , Wang Yuhua , Zhang Qi , Jia Miao , Li Mingzhe , Chen Yiling , Cheng Pengyuan , Hong Lei , Zhang Ying , Ye Jianghua , Wang Haibin
Published: June 2024

There are obvious differences in quality between different varieties of the same plant, and it is not clear whether they can be effectively distinguished from each other from a bacterial point of view. In this study, 44 tea tree varieties (Camellia sinensis) were used to analyze the rhizosphere soil bacterial community using high-throughput sequencing technology, and five types of machine deep learning were used for modeling to obtain characteristic microorganisms that can effectively differentiate different varieties, and validation was performed. The relationship between characteristic microorganisms, soil nutrient transformation, and tea quality formation was further analyzed. It was found that 44 tea tree varieties were classified into two groups (group A and group B) and the characteristic bacteria that distinguished them came from 23 genera. Secondly, the content of rhizosphere soil available nutrients (available nitrogen, available phosphorus, and available potassium) and tea quality indexes (tea polyphenols, theanine, and caffeine) was significantly higher in group A than in group B. The classification result based on both was consistent with the above bacteria. This study provides a new insight and research methodology into the main reasons for the formation of quality differences among different varieties of the same plant.
Tissue-Specific Tolerance to High-Temperature and Nutrient-Poor Conditions in a Canopy-Forming Macroalga, Surviving at an Ocean Warming Hotspot
Authors: Endo Hikaru , Kodama Masafumi , Kawashima Ryoya , Kumagai Momochika , Matsuoka Midori , Ebata Keigo , Okunishi Suguru
Published: June 2024

Most canopy-forming macroalgae have disappeared from temperate reefs in southern Japan, one of the ocean warming hotspots, but Sargassum nipponicum is surviving in this region. As this species’ annual shoots emerge from holdfasts during summer, both plant components may be highly tolerant to warm and nutrient-poor conditions in this season. The present study examined the effects of temperature and nutrient conditions on holdfast growth, shoot emergence from holdfasts, and shoot growth in S. nipponicum samples collected in Tanegashima Island, southern Japan. The summer temperature in this region (30 °C) allowed holdfast growth and shoot emergence but inhibited shoot growth. Nutrient-poor conditions had limited effects on the first two parameters but suppressed shoot growth. These results suggested that during warm summers and under nutrient-poor conditions in southern Japan, shoots can emerge from S. nipponicum holdfasts but cannot further grow. Additionally, nutrient loading from a nearby river was higher at the only site dominated by S. nipponicum, than at the other sites where this species was absent on Tanegashima Island. This was observed especially between autumn and winter, implying that such a nutrient-rich environment may contribute to shoot growth in S. nipponicum and to the persistence of its population in the area.
Amazon Rainforest Hidden Volatiles—Part I: Unveiling New Compounds from Acmella oleracea (L.) R.K. Jansen Essential Oil
Authors: Radulović Niko S. , Mladenović Marko Z. , Lima Clarissa Silva , Müller Elza Caroline Alves , da Costa Elizabeth Vianna Moraes , Martins Rozilene Valadares , Boylan Fabio
Published: June 2024

Motivated by the culinary and ethnopharmacological use of Acmella oleracea (L.) R.K. Jansen, this study aimed to unveil new chemical compounds from its essential oil (EO). Acmella oleracea, known for its anesthetic and spicy properties, has been used in traditional medicine and cuisine, particularly in Northern Brazil. Through a detailed GC-MS analysis, 180 constituents were identified, including 12 tentatively identified long-chain α-keto esters of various acids. Additionally, 18 new esters were synthesized for structural verification. This research expands the known chemical diversity of A. oleracea EO, providing a basis for potential pharmacological applications. The identification of new natural products, including homologs and analogs of acmellonate, underscores the EO’s rich chemical profile and its potential for novel bioproduct development.
A Physiological and Molecular Docking Insight on Quercetin Mediated Salinity Stress Tolerance in Chinese Flowering Cabbage and Increase in Glucosinolate Contents
Authors: Akram Waheed , Khan Imran , Rehman Areeba , Munir Bareera , Guo Juxian , Li Guihua
Published: June 2024

The present study was performed to investigate the negative impact of salinity on the growth of Chinese flowering cabbage (Brassica rapa ssp. chinensis var. parachinensis) and the ameliorative effects of quercetin dihydrate on the plant along with the elucidation of underlying mechanisms. The tolerable NaCl stress level was initially screened for the Chinese flowering cabbage plants during a preliminary pot trial by exposing the plants to salinity levels (0, 50, 100, 150, 200, 250, 300, 350, and 400 mM) and 250 mM was adopted for further experimentation based on the findings. The greenhouse experiment was performed by adopting a completely randomized design using three different doses of quercetin dihydrate (50, 100, 150 µM) applied as a foliar treatment. The findings showed that the exposure salinity significantly reduced shoot length (46.5%), root length (21.2%), and dry biomass (32.1%) of Chinese flowering cabbage plants. Whereas, quercetin dihydrate applied at concentrations of 100, and 150 µM significantly diminished the effect of salinity stress by increasing shoot length (36.8- and 71.3%), root length (36.57- and 56.19%), dry biomass production (51.4- and 78.6%), Chl a (69.8- and 95.7%), Chl b (35.2- and 87.2%), and carotenoid contents (21.4- and 40.3%), respectively, compared to the plants cultivated in salinized conditions. The data of physiological parameters showed a significant effect of quercetin dihydrate on the activities of peroxidase, superoxide dismutase, and catalase enzymes. Interestingly, quercetin dihydrate increased the production of medicinally important glucosinolate compounds in Chinese flowering cabbage plants. Molecular docking analysis showed a strong affinity of quercetin dihydrate with three different stress-related proteins of B. rapa plants. Based on the findings, it could be concluded that quercetin dihydrate can increase the growth of Chinese flowering cabbage under both salinity and normal conditions, along with an increase in the medicinal quality of the plants. Further investigations are recommended as future perspectives using other abiotic stresses to declare quercetin dihydrate as an effective remedy to rescue plant growth under prevailing stress conditions.
Cheungsam Seed Husk Extract Reduces Skin Inflammation through Regulation of Inflammatory Mediator in TNF-α/IFN-γ-Induced HaCaT Cells
Authors: Han Ji-Ye , Lee Yun Jung , Lim Do-Won , Jung Hyun-Ju , Kwon EunJeong , Hong Jongki , Lee Young-Mi
Published: June 2024

Cannabis contains numerous natural components and has several effects such as anticancer, anti-inflammatory and antioxidant. Cheungsam is a variety of non-drug-type hemp, developed in Korea and is used for fiber (stem) and oil (seed). The efficacy of Cheungsam on skin is not yet known, and although there are previous studies on Cheungsam seed oil, there are no studies on Cheungsam seed husk. In this study, we investigated the potential of Cheungsam seed husk ethanol extract (CSSH) to alleviate skin inflammation through evaluating the gene and protein expression levels of inflammatory mediators. The results showed that CSSH reduced pro-inflammatory cytokines (IL-1β, IL-6, IL-8, MCP-1 and CXCL10) and atopic dermatitis-related cytokines (IL-4, CCL17, MDC and RANTES) in TNF-α/IFN-γ-induced HaCaT cells. Furthermore, ERK, JNK and p38 phosphorylation were decreased and p-p65, p-IκBα, NLRP3, caspase-1, p-JAK1 and p-STAT6 were suppressed after CSSH treatment. CSSH significantly increased the level of the skin barrier factors filaggrin and involucrin. These results suggest that Cheungsam seed husk ethanol extract regulates the mechanism of skin inflammation and can be used as a new treatment for skin inflammatory diseases.
Considerations on Prevention of Pollution from Ships in a Seaport
Authors: Đelović Deda
Published: June 2024

Negative effects on the port environment can originate from ports’ hinterland, ports’ activities and operations, and from ships. According to the available literature and long-lasting experience of numerous ports presented in different sources, pollution from ships belongs to the group of environmental priorities in ports. After a theoretical introduction where the importance of ports, their development, and the challenges/risks faced by ports (with special attention to environmental risks) are analyzed, an overview of the literature from the domain of prevention of pollution from ships is presented. Based on the standard structure of the PDCA (Plan, Do, Check, Act) management cycle, in this paper, a process model of the management (sub)system of prevention of pollution from ships in a port is proposed. Key demands related to the modeled (sub)system and bases which directly determine those demands are made concrete through an analysis of a case study: the Port of Bar (Montenegro). A categorization of domains of risks of pollution from ships in the analyzed port is conducted using the Analytic Hierarchy Process (AHP) method: considerations are based on a three-level hierarchy model: “0”—goal; level “1”—criterion; and level “2”—choice. The results of the analyses show that a domain with the highest rank of risk of pollution from ships is variant (alternative) M1: unloading oil/oil derivatives from ships to tanks (and vice versa), with a composite weight of 0.5365 (53.65%). The results of considerations presented in this paper can be used in a process of optimization of the (sub)system of prevention of pollution from ships in a multipurpose port as well as a reliable base for further research in this domain.
Assessing Energy Consumption, Carbon Emissions, and Costs in Biomass-to-Gas Processes: A Life-Cycle Assessment Approach
Authors: Liu Minwei , Zeng Jincan , Huang Guori , Liu Xi , He Gengsheng , Yao Shangheng , Shang Nan , Zheng Lixing , Wang Peng
Published: June 2024

China has a huge potential for biomass utilization. Converting low-grade biomass into high-quality hydrogen and natural gas is of great significance in promoting the utilization of biomass resources and the achievement of carbon reduction goals. Based on the data of biomass collection, transportation, power generation, hydrogen production and gas production stages in China, this paper constructs a multi-chain hybrid whole-life process evaluation model for “electricity to gas” and comprehensively compares the energy consumption, carbon emission and cost of the two chains of “hydrogen production from biomass power generation” and “methane production from biomass power generation”. We comprehensively compare the energy consumption, carbon emissions and costs of biomass-to-hydrogen and biomass-to-methane technologies. Biomass natural gas is found to have significant advantages in terms of energy consumption, carbon emissions and economics compared to biomass hydrogen production. In order to promote the development of the biomass “electricity to gas” industry in China, this paper proposes that PEM electrolysis tanks can be used for hydrogen production, and the distance from the biomass feedstock collection to the hydrogen production chemical park should be optimized to reduce the whole-life-cycle cost. Biomass natural gas can buy time for the development of China’s hydrogen industry and infrastructure construction.
Connectedness of Carbon Price and Energy Price under Shocks: A Study Based on Positive and Negative Price Volatility
Authors: Yu Bo , Chang Zhijia
Published: June 2024

We calculate both positive and negative price volatilities based on Realized Semi-variance (RS) in major economies’ carbon and energy markets with daily data from 1 July 2013 to 31 May 2023. Subsequently, we construct a network using the Elastic-Net-VAR model to analyze the contagion of price volatilities and examine how shocks affect the connectedness between these markets’ price volatilities using Local Projection. The following findings are presented: (1) There exists a robust correlation between carbon price volatilities and energy price volatilities, with time-varying overall network connectedness ranging from 21.54% to 83.34%. (2) Carbon markets primarily act as recipients of price volatilities, while energy markets serve as initiators. (3) The spillover effects and inflow of negative price volatilities are more pronounced compared to those of positive price volatilities. This is attributed to the fact that declining prices often indicate a market downturn, leading to the easy dissemination of adverse news across interconnected markets. Concurrently, increasing fragility diminishes its resilience against risks. (4) Shocks have a significant influence on the connectedness between carbon prices and energy prices, with different mechanisms at play under different shocks. The COVID-19 pandemic has increased the connectedness between carbon markets and energy markets primarily through common exposure mechanisms. Conversely, geopolitical risks reduce network connectedness by decreasing price complementarity.
Integrating Trade-In Strategies for Optimal Pre-Positioning Decisions in Relief Supply-Chain Systems
Authors: Ju Yingjie , Hou Hanping , Yang Jianliang , Ren Yuheng , Yang Jimei
Published: June 2024

This paper delves into optimizing the rotation of relief supplies within the relief supply chain system, concentrating on reserve quantity decisions for governments and humanitarian organizations involved in disaster response. By integrating a trade-in strategy with suppliers, it ensures a precise and timely response to the fluctuating demand for relief supplies post-disaster. Utilizing the newsvendor model, optimization theory, and supply chain coordination principles, we developed a comprehensive model that calculates optimal reserve quantities for pre-positioning demanders. It also outlines the expected profit function for suppliers and a robust supply chain coordination model. The findings highlight that optimal stockpiling decisions for relief supplies are heavily influenced by cost parameters, material characteristics, and the relationship between trade-in pricing and market resale values. Notably, higher trade-in prices generally reduce the government’s optimal reserve quantities, impacting strategic decisions within supply chain coordination. This research adds to disaster management literature by offering strategic insights into how coordination and pricing strategies can improve disaster preparedness and response efficiency and effectiveness.
Network science to correlate COVID-19 and tourism indicators in Mexico
Authors: Arenas-Resendiz Tanya , Patiño-Ortiz Julián , Martínez-Cruz Miguel Ángel , Dorantes-Benavidez Humberto , Chávez-Pichardo Mauricio
Published: June 2024

In this paper we analyze tourism as complex system susceptible to external perturbations, like COVID-19 public health emergency. The research objective is to confirm pertinence of using transdisciplinary tools such as complexity approach and network analysis to understand and represent tourism occupancy dynamic. We used network science methodology to introduce an analysis that integrates two Mexican tourism industry indicators: Tourist Destinations occupancy rates and Hospitality-Gastronomy jobs; correlated with COVID-19 in Mexico pandemic indicator: Confirmed cases. The analysis results are based on centrality measures used to describe organizational patterns in tourism dynamic, besides we identified some generic properties of tourism occupancy distribution.
Digital educational environments: strategies and tools for training competent professionals
Authors: Hrechanyk Nataliia , Eganov Alexsandr , Bandura Victor , Aramyan Armen , Shijia He
Published: June 2024

The article highlights digital educational environments, describes strategies and tools for training competent specialists. Ways of using digital technologies in education and their didactic possibilities are revealed, key characteristics are listed. The purpose and tasks of the digital educational environment of educational institutions of modern society are specified. The process of building a digital educational environment of an educational institution is shown. The most important software of the information system of professional training, which should be in the information and digital educational environment of the educational institution, is highlighted. The necessary conditions and organizational principles of creating an effective digital educational environment of an educational institution are substantiated. The main goals and recommended tasks regarding the formation of a digital educational environment in an educational institution are proposed. An experimental study was conducted to find out the level of digital competence of future specialists and the role of the digital educational environment in the educational institution.