bluuebunny/crossref_metadata_2025 huggingface dataset:
Induced Resistance by Ascorbate Oxidation Involves Potentiating of the Phenylpropanoid Pathway and Improved Rice Tolerance to Parasitic Nematodes
Authors: Singh Richard Raj , Pajar Jessil Ann , Audenaert Kris , Kyndt Tina
Published: August 2021

Anticipating an increased ecological awareness, scientists have been exploring new strategies to reduce the use of chemical pesticides to control pests and diseases. Triggering the intrinsic plant defense system is one of the promising strategies to reduce yield loss by pathogenic organisms, such as nematodes. Ascorbate oxidase (AO) enzyme plays an important role in plant defense by regulating the apoplastic ascorbate/dehydroascorbate (DHA) ratio via the ascorbate oxidation process. Ascorbate oxidation is known to induce systemic resistance in rice against parasitic root-knot nematodes (RKN). Here, we sought to evaluate if AO- or DHA-induced resistance (IR) against RKN M. graminicola involves activation of the phenylpropanoid pathway and whether this IR phenotype has potential effects on growth of rice seedlings under stressed and unstressed conditions. Our results show that AO/DHA-IR against these parasitic nematodes is dependent on activation of phenylalanine ammonia lyase (PAL). However, application of reduced ascorbic acid (AA) did not induce this response. Gene expression analysis via qRT-PCR showed that OsPAL2 and OsPAL4 are highly expressed in AO/DHA-sprayed nematode-infected roots and PAL-activity measurements confirmed that AO/DHA spraying triggers the plants for primed activation of this enzyme upon nematode infection. AO/DHA-IR is not effective in plants sprayed with a chemical PAL inhibitor confirming that AO/DHA-induced resistance is dependent on PAL activity. Improved plant growth and low nematode infection in AO/DHA-sprayed plants was found to be correlated with an increase in shoot chlorophyll fluorescence (Fv/Fm), chlorophyll index (ChlIdx), and modified anthocyanin reflection index which were proven to be good above-ground parameters for nematode infestation. A detailed growth analysis confirmed the improved growth of AO/DHA-treated plants under nematode-infected conditions. Taken together, our results indicate that ascorbate oxidation enhances the phenylpropanoid-based response to nematode infection and leads to a tolerance phenotype in treated rice plants.
LFR Physically and Genetically Interacts With SWI/SNF Component SWI3B to Regulate Leaf Blade Development in Arabidopsis
Authors: Lin Xiaowei , Yuan Can , Zhu Bonan , Yuan Tingting , Li Xiaorong , Yuan Shan , Cui Sujuan , Zhao Hongtao
Published: August 2021

Leaves start to develop at the peripheral zone of the shoot apical meristem. Thereafter, symmetric and flattened leaf laminae are formed. These events are simultaneously regulated by auxin, transcription factors, and epigenetic regulatory factors. However, the relationships among these factors are not well known. In this study, we conducted protein-protein interaction assays to show that our previously reported Leaf and Flower Related (LFR) physically interacted with SWI3B, a component of the ATP-dependent chromatin remodeling SWI/SNF complex in Arabidopsis. The results of truncated analysis and transgenic complementation showed that the N-terminal domain (25–60 amino acids) of LFR was necessary for its interaction with SWI3B and was crucial for LFR functions in Arabidopsis leaf development. Genetic results showed that the artificial microRNA knockdown lines of SWI3B (SWI3B-amic) had a similar upward-curling leaf phenotype with that of LFR loss-of-function mutants. ChIP-qPCR assay was conducted to show that LFR and SWI3B co-targeted the promoters of YABBY1/FILAMENTOUS FLOWER (YAB1/FIL) and IAA carboxyl methyltransferase 1 (IAMT1), which were misexpressed in lfr and SWI3B-amic mutants. In addition, the association between LFR and the FIL and IAMT1 loci was partly hampered by the knockdown of SWI3B. These data suggest that LFR interacts with the chromatin-remodeling complex component, SWI3B, and influences the transcriptional expression of the important transcription factor, FIL, and the auxin metabolism enzyme, IAMT1, in flattened leaf lamina development.
A Novel and Highly Effective Bayesian Sampling Algorithm Based on the Auxiliary Variables to Estimate the Testlet Effect Models
Authors: Lu Jing , Zhang Jiwei , Zhang Zhaoyuan , Xu Bao , Tao Jian
Published: August 2021

In this paper, a new two-parameter logistic testlet response theory model for dichotomous items is proposed by introducing testlet discrimination parameters to model the local dependence among items within a common testlet. In addition, a highly effective Bayesian sampling algorithm based on auxiliary variables is proposed to estimate the testlet effect models. The new algorithm not only avoids the Metropolis-Hastings algorithm boring adjustment the turning parameters to achieve an appropriate acceptance probability, but also overcomes the dependence of the Gibbs sampling algorithm on the conjugate prior distribution. Compared with the traditional Bayesian estimation methods, the advantages of the new algorithm are analyzed from the various types of prior distributions. Based on the Markov chain Monte Carlo (MCMC) output, two Bayesian model assessment methods are investigated concerning the goodness of fit between models. Finally, three simulation studies and an empirical example analysis are given to further illustrate the advantages of the new testlet effect model and Bayesian sampling algorithm.
Association Between Daily Worry, Pathological Worry, and Fear of Progression in Patients With Cancer
Authors: Dinkel Andreas , Marten-Mittag Birgitt , Kremsreiter Katrin
Published: August 2021

Background: Fear of progression (FoP), or fear of cancer recurrence (FCR), is characterized by worries or concerns about negative illness-related future events. Actually, to worry is a common cognitive process that, in its non-pathological form, belongs to daily life. However, worry can also become pathological appearing as a symptom of mental disorders. This study aimed at investigating the associations among daily worry, pathological worry, and FoP in patients with cancer.

Methods: This is a cross-sectional study that includes 328 hospitalized patients with cancer. Patients filled out the FoP Questionnaire (FoP-Q), the Worry Domains Questionnaire (WDQ) for the assessment of daily worry, and the Penn State Worry Questionnaire (PSWQ) for the assessment of pathological worry. Depressive, anxiety, and somatic symptoms were measured with modules of the Patient Health Questionnaire [Patient Health Questionnaire-Depressive Symptoms (PHQ-2), Generalized Anxiety Disorder-2 (GAD-2), and Patient Health Questionnaire-Somatic Symptoms (PHQ-15)]. Furthermore, a structured clinical interview was conducted for the assessment of anxiety disorders. The hierarchical multiple linear regression analysis was used to identify factors independently associated with FoP.

Results: Mean age of the participants was M = 58.5 years (SD = 12.8), and 64.6% were men. FoP and worry were significantly intercorrelated (r = 0.58–0.78). The level of FoP was most strongly associated with daily worry (β = 0.514, p < 0.001), followed by pathological worry (β = 0.221, p < 0.001). Further significant determinants were younger age and depressive and anxiety symptoms. Clinical variables were not independently associated with FoP. The final model explained 74% of the variance.

Discussion: Fear of progression is strongly associated with daily worry and pathological worry. These results bring up the question of whether FoP is an expression of a general tendency to worry. Whether a general tendency to worry, in fact, represents an independent vulnerability factor for experiencing FCR/FoP needs to be investigated in a longitudinal research design.
Personal and Social Responsibility Development in a Volleyball Hybrid Positive Youth Development Program: A Mixed Methods Approach
Authors: Muñoz-Llerena Antonio , Hernández-Hernández Elena , García-de-Alcaraz Antonio , Caballero-Blanco Pablo
Published: August 2021

Positive Youth Development (PYD) programs are being used to foster holistic growth in children and adolescents. The hybridized application of two or more programs of this type has acquired special relevance in recent years. Although their application is common in the school context, there are few research studies that attempt their implementation in an extracurricular context. This study analyzed the effects of an intervention based on a hybrid PYD program on personal responsibility (PR) and social responsibility (SR) in youth volleyball players in an extracurricular context. A hybrid program was applied during the competitive season, with a total of 37 sessions with 30 girl students (15 experimental and 15 control) aged between 8 and 10 years (M = 8.87, SD = 0.82). A convergent mixed methods design was applied to integrate the following: (a) semi-structured interviews and field notes and (b) personal and social responsibility questionnaires. The results indicated that the implementation of the hybrid program appeared to yield a positive perception of learning in both the participants and the coach. Although there were no statistically significant intergroup or intragroup differences, the findings suggest that the hybrid program seems to be effective in fostering PR and SR in youth girl volleyball players.
Drivers of Subjective Well-Being Under Different Economic Scenarios
Authors: Arrondo Rubén , Cárcaba Ana , González Eduardo
Published: August 2021

This paper explores the evolution of the driving forces that shape individual subjective well-being (SWB) in Spain from 2013 to 2018. Several socio-demographic, material conditions and quality of life (QoL) variables are considered as potential drivers of SWB. The data come from a large survey carried in two different time periods. The first one (2013) is characterized by a negative economic scenario as a result of the global financial crisis of 2008. The second one (2018) is characterized by fast economic recovery. Our results suggest that the material conditions variables, especially unemployment, have a much deeper impact on SWB during economic downturns than during economic recovery periods. Social connections and health status are determinant factors behind SWB, especially if the economy is working well. Our results also point to changes in gender effects. While women were happier than men in 2013, this effect disappears in 2018. Paradoxically, this suggest an increase in female expectations about their own lives during this period.
Time Spent on School-Related Activities at Home During the Pandemic: A Longitudinal Analysis of Social Group Inequality Among Secondary School Students
Authors: Zinn Sabine , Bayer Michael
Published: August 2021

Substantial educational inequalities have been documented in Germany for decades. In this article, we examine whether educational inequalities among children have increased or remained the same since the school closures of spring 2020 due to the COVID-19 pandemic. Our perspective is longitudinal: We compare the amount of time children in secondary schools spent on school-related activities at home before the pandemic, during school closures, and immediately after returning to in-person learning. We operationalize family socio-economic status using the highest parental educational attainment. Based on the theoretical assumption that the pandemic affected everyone equally, we formulate a hypothesis of equalization during the first period of school closures. For the period thereafter, however, we assume that parents with a low level of education had more difficulties bearing the additional burden of supervising and supporting their children’s learning activities. Thus, for that period, we postulate an increase in educational inequality. To study our hypotheses, we use data from the 2019 wave of the SOEP and the SOEP-CoV study, both of which are probability samples. The SOEP-CoV study provides a unique database, as it was conducted during the lockdown of spring 2020 and in the following month. For statistical analysis, we use probit regressions at three measurement points (in 2019, in 2020 during the school closures, and in the month after closures). The comparison of these three time points makes our analysis and findings unique in the research on education during the COVID-19 pandemic, in particular with regard to Germany-wide comparisons. Our results confirm the hypothesis of equalization during the first school closures and the hypothesis of an increase in educational in the subsequent period. Our findings have direct policy implications regarding the need to further expand support systems for children.
Beliefs About Transitional Events: The Effect of Experience and Life-Script Consistency
Authors: Shi Liangzi , Brown Norman R.
Published: August 2021

The present study examined the beliefs about two types of important life transitions: transitions that are consistent with the cultural life script (e.g., getting married) and transitions that diverge from it (e.g., relocating). Data were collected from two conditions: individuals in the experienced condition only responded to transitions they had experienced; individuals in the hypothetical condition provided ratings only for transitions they had not experienced. Participants rated the likelihood and typical age of occurrence, importance, transitional impact, and valence for an individualized set of condition-appropriate events. We found that script-consistent events were considered more normative and positive than script-divergent events. The two types of events, however, differed little in terms of importance or transitional impact. We conclude by arguing that although script-consistent and script-divergent transitions have much in common from a mnemonic perspective, the distinction is still warranted in the context of lifetime planning and evaluation.
Is FMR1 CGG Repeat Number Polymorphism Associated With Phenotypic Variation in the General Population? Report From a Cohort of 5,499 Adults
Authors: Hong Jinkuk , DaWalt Leann , Baker Mei Wang , Berry-Kravis Elizabeth M. , Mailick Marsha R.
Published: August 2021

FMR1 CGG repeat length was assayed in 5499 research participants (2637 men and 2862 women) in the Wisconsin Longitudinal Study (WLS), a population-based cohort. Most past research has focused on clinically-ascertained individuals with expansions in CGG repeats, either those with fragile X syndrome (> 200 CGG repeats), the FMR1 premutation (55–200 repeats), or in the gray zone (variously defined as 45–54 or 41–54 repeats). In contrast, the WLS is a unique source of data that was obtained from an unselected cohort of individuals from the general population for whom FMR1 CGG repeat length was assayed. The WLS is a random sample of one-third of all high school seniors in the state of Wisconsin in 1957. The most recent round of data collection was in 2011; thus, the study spanned over 50 years. Saliva samples were obtained from 69% of surviving members of the cohort in 2008 and 2011, from which CGG repeats were assayed. With one exception, the CGG repeat length of all members of this cohort was below 100 (ranging from 7 to 84). The present study evaluated the genotype-phenotype associations of CGG repeat number and IQ, college graduation, age at menopause, number of biological children, having a child with intellectual or developmental disabilities, and the likelihood of experiencing an episode of depression during adulthood. Linear and curvilinear effects were probed. Although effect sizes were small, significant associations were found between CGG repeat length and high school IQ score, college graduation, number of biological children, age at menopause, and the likelihood of having an episode of depression. However, there was no significant association between repeat length and having a child diagnosed with an IDD condition. This study demonstrates a continuum of phenotype effects with FMR1 repeat lengths and illustrates how research inspired by a rare genetic condition (such as fragile X syndrome) can be used to probe genotype-phenotype associations in the general population.
Female Community Health Workers and Health System Navigation in a Conflict Zone: The Case of Afghanistan
Authors: Parray Ateeb Ahmad , Dash Sambit , Ullah Md. Imtiaz Khalil , Inam Zuhrat Mahfuza , Kaufman Sophia
Published: August 2021

Afghanistan ranked 171st among 188 countries in the Gender Inequality Index of 2011 and has only 16% of its women participating in the labor force. The country has been mired in violence for decades which has resulted in the destruction of the social infrastructure including the health sector. Recently, Afghanistan has deployed community health workers (CHW) who make up majority of the health workforce in the remote areas of this country. This paper aims to bring the plight of the CHWs to the forefront of discussion and shed light on the challenges they face as they attempt to bring basic healthcare to people living in a conflict zone. The paper discusses the motivations of Afghani women to become CHWs, their status in the community and within the health system, the threatening situations under which they operate, and the challenges they face as working women in a deeply patriarchal society within a conflict zone. The paper argues that female CHWs should be provided proper accreditation for their work, should be allowed and encouraged to progress in their careers, and should be instilled at the heart of healthcare program planning because they have the field experience to make the most effective and community oriented programmatic decisions.
The Predictive Role of Lymphocyte-to-Monocyte Ratio in Acute Kidney Injury in Acute Debakey Type I Aortic Dissection
Authors: Ma Xiaochun , Chen Shanghao , Yun Yan , Zhao Diming , Li Jinzhang , Wu Zezhong , Liu Yanwu , Shen Hechen , Ma Huibo , Wang Zhengjun , Zou Chengwei , Zhang Haizhou
Published: August 2021

Background: The post-operative acute kidney injury (AKI) represents a common complication in the Acute Debakey Type I Aortic Dissection (ADTIAD) and predicts a poorer prognosis. The clinical evidence is scarce supporting the predictive value of the pre-operative lymphocyte-to-monocyte ratio (LMR) in post-operative AKI in ADTIAD.

Methods: In this retrospective cohort study, 190 consecutive patients with ADTIAD enrolled for surgical treatment between January 1, 2013, and December 31, 2018. The diagnosis of AKI followed the Kidney Disease: Improving Global Outcomes guidelines (KDIGO). Pre-operative LMR and other possible risk factors were analyzed for their prognostic value in the post-operative AKI in ADTIAD.

Results: The subjects were assigned to the low-LMR and high-LMR groups according to the median value of pre-operative LMR. For post-operative AKI, the incidence and the severity in the low-LMR group were statistically different from that of the high-LMR group. Besides, the lower LMR was statistically associated with the more extended ICU stay and intubation time and higher incidences of ischemic stroke and in-hospital mortality. Additionally, in the multivariable analysis, the pre-operative LMR was an independent predictor for post-operative AKI in ADTIAD. A predictive model for post-operative AKI in ADTIAD was established incorporating LMR.

Conclusions: LMR is an independent prognostic indicator incorporated into the predictive model with other risk factors to predict the post-operative AKI in ADTIAD.
A Simulation Study of the Use of Vaccination to Control Foot-and-Mouth Disease Outbreaks Across Australia
Authors: Capon Tim R. , Garner Michael G. , Tapsuwan Sorada , Roche Sharon , Breed Andrew C. , Liu Shuang , Miller Corissa , Bradhurst Richard , Hamilton Sam
Published: August 2021

This study examines the potential for foot-and-mouth disease (FMD) control strategies that incorporate vaccination to manage FMD spread for a range of incursion scenarios across Australia. Stakeholder consultation was used to formulate control strategies and incursion scenarios to ensure relevance to the diverse range of Australian livestock production regions and management systems. The Australian Animal Disease Spread model (AADIS) was used to compare nine control strategies for 13 incursion scenarios, including seven control strategies incorporating vaccination. The control strategies with vaccination differed in terms of their approaches for targeting areas and species. These strategies are compared with two benchmark strategies based on stamping out only. Outbreak size and duration were compared in terms of the total number of infected premises, the duration of the control stage of an FMD outbreak, and the number of vaccinated animals. The three key findings from this analysis are as follows: (1) smaller outbreaks can be effectively managed by stamping out without vaccination, (2) the size and duration of larger outbreaks can be significantly reduced when vaccination is used, and (3) different vaccination strategies produced similar reductions in the size and duration of an outbreak, but the number of animals vaccinated varied. Under current international standards for regaining FMD-free status, vaccinated animals need to be removed from the population at the end of the outbreak to minimize trade impacts. We have shown that selective, targeted vaccination strategies could achieve effective FMD control while significantly reducing the number of animals vaccinated.
Design and Characterization of an Electrostatic Constant-Force Actuator Based on a Non-Linear Spring System
Authors: Thewes Anna Christina , Schmitt Philip , Löhler Philipp , Hoffmann Martin
Published: August 2021

In recent years, tissue engineering with mechanical stimulation has received considerable attention. In order to manipulate tissue samples, there is a need for electromechanical devices, such as constant-force actuators, with integrated deflection measurement. In this paper, we present an electrostatic constant-force actuator allowing the generation of a constant force and a simultaneous displacement measurement intended for tissue characterization. The system combines a comb drive structure and a constant-force spring system. A theoretical overview of both subsystems, as well as actual measurements of a demonstrator system, are provided. Based on the silicon-on-insulator technology, the fabrication process of a moveable system with an extending measurement tip is shown. Additionally, we compare measurement results with simulations. Our demonstrator reaches a constant-force of 79 ± 2 μN at an operating voltage of 25 V over a displacement range of approximately 40 μm, and the possibility of adjusting the constant-force by changing the voltage is demonstrated.
Efficacy of Herbicides in Controlling Wild Onion (Asphodelus tenuifolius L.) in Cumin Grown under Arid Climatic Conditions
Authors: Mehriya Moti Lal , Geat Neelam , Kumhar Sita Ram , Alrajhi Abdullah A. , Alkuriji Mohammed A. , Dewidar Ahmed Z. , Mattar Mohamed A.
Published: August 2021

A field experiment was conducted to investigate the effectiveness of different herbicides for controlling wild onion (Asphodelus tenuifolius) in cumin (Cuminum cyminum L.) during the rabi seasons (2018–2019 and 2019–2020) at Agricultural Research Station, Agriculture University, Jodhpur, Rajasthan. The experiment comprised eight herbicidal weed management treatments for wild onion applied to cumin in a three-replication randomized block design. Among the herbicidal weed management treatments, early post-emergence (8 DAS) application of oxyfluorfen 200 g/ha resulted in the lowest weed density and dry matter of Asphodilus tenuifolius, with maximum weed (Asphodilus tenuifolius) control efficiency at 40 days after sowing (DAS) during both experimental years. Likewise, the highest total efficiency of weed control was recorded with the application of oxyfluorfen 200 g/ha at 8 DAS. Oxyflourfen 200 g/ha used early post emergence (8 DAS) reduced the weed index more effectively than the other herbicides. It also recorded the highest number of branches/plant, plant height, umbels/plant, umbellates/umbel, seeds/umbellates, and seed yield. However, application of oxyflourfen @ 200 g/ha 8 DAS–early POE and pendimethalin 38.7 CS 500 g/ha + oxyfluorfen @ 150 g/ha 8 DAS–early POE were statistically similar in terms of plant growth, yield, and yield attributes. The net returns (366.49 USD/ha in 2018–2019 and 175.72 USD/ha in 2019–2020) and B:C ratio (1.70 and 1.33 in 2018–2019 and 2019–2020, respectively) were also superior, with oxyfluorfen 200 g/ha applied early post emergence.
Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle
Authors: Elayadeth-Meethal Muhammed , Thazhathu Veettil Aravindakshan , Asaf Muhasin , Pramod Sathiamoorthy , Maloney Shane K. , Martin Graeme B. , Rivero M. Jordana , Sejian Veerasamy , Naseef Punnoth Poonkuzhi , Kuruniyan Mohamed Saheer , Lee Michael R. F.
Published: August 2021

Climate change is an imminent threat to livestock production. One adaptation strategy is selection for heat tolerance. While it is established that the ATP1A1 gene and its product play an important role in the response to many stressors, there has been no attempt to characterize the sequence or to perform expression profiling of the gene in production animals. We undertook a field experiment to compare the expression profiles of ATP1A1 in heat-tolerant Vechur and Kasaragod cattle (Bos taurus indicus) with the profile of a heat-susceptible crossbreed (B. t. taurus × B. t. indicus). The cattle were exposed to heat stress while on pasture in the hot summer season. The environmental stress was quantified using the temperature humidity index (THI), while the heat tolerance of each breed was assessed using a heat tolerance coefficient (HTC). The ATP1A1 mRNA of Vechur cattle was amplified from cDNA and sequenced. The HTC varied significantly between the breeds and with time-of-day (p < 0.01). The breed–time-of-day interaction was also significant (p < 0.01). The relative expression of ATP1A1 differed between heat-tolerant and heat-susceptible breeds (p = 0.02). The expression of ATP1A1 at 08:00, 10:00 and 12:00, and the breed–time-of-day interaction, were not significant. The nucleotide sequence of Vechur ATP1A1 showed 99% homology with the B. t. taurus sequence. The protein sequence showed 98% homology with B. t. taurus cattle and with B. grunniens (yak) and 97.7% homology with Ovis aries (sheep). A molecular clock analysis revealed evidence of divergent adaptive evolution of the ATP1A1 gene favoring climate resilience in Vechur cattle. These findings further our knowledge of the relationship between the ATP1A1 gene and heat tolerance in phenotypically incongruent animals. We propose that ATP1A1 could be used in marker assisted selection (MAS) for heat tolerance.
Horse Behavior towards Familiar and Unfamiliar Humans: Implications for Equine-Assisted Services
Authors: Brubaker Lauren , Schroeder Katy , Sherwood Dawn , Stroud Daniel , Udell Monique A. R.
Published: August 2021

While human benefits of animal-assisted therapy programs have been documented, relatively little research has been conducted on behavioral factors that predict a successful equine-assisted services (EAS) horse. This study compares the behavior of experienced and non-experienced EAS horses as well as horses selected for future EAS work in a series of sociability and temperament tests. No significant differences were found between experienced and non-experienced horses in the sociability measures or for most of the temperament tests; however, significant differences were found between groups in the brushing test, with non-experienced horses showing more affiliative behaviors towards the familiar handler and unfamiliar persons. No significant differences were found between selected and non-selected horses in the temperament tests. However, non-selected horses were found to show significantly more affiliative behaviors towards a familiar person during a sociability test compared with selected horses. These findings suggest that the social behavior and temperament of EAS horses may not be significantly different from other available horses not selected for EAS work. Instead, these decisions may primarily reflect subjective impressions of fit. Interestingly, on measures where significant differences were identified, the horses not actively engaged in or selected for therapy were the ones that showed greater affiliative responses to familiar and unfamiliar humans. Reasons for why this may be, as well as future directions in EAS selection, are discussed.
Lying Behaviour in Dairy Goats: Effects of a New Automated Feeding System Assessed by Accelerometer Technology
Authors: Maurmann Ines , Greiner Bianca A. E. , von Korn Stanislaus , Bernau Maren
Published: August 2021

The aim of this study was to evaluate lying behaviour in dairy goats before and after installation of an optimized automatic concentrated feeding system (AFS). A mixed-horned herd of Bunte Deutsche Edelziege was used. As many agonistic interactions between goats happen at the feeding place, a new automated feeding system was installed to better fulfil the needs of horned goats. Lying behaviour is an indicator to ascertain animal welfare of ruminants. In order to measure lying behaviour accelerometer technology was used and verified by video analyses. The results show an agreement of 99.62–99.93% per lying time by comparing accelerometers to video data. Over all goats, a mean ± SD lying time (LT) of 11.78 ± 1.47 h/d, a mean ± SD lying bout duration (LBD) of 0.51 ± 0.10 h/bout and a mean ± SD frequency of lying bouts (FLB) of 24.35 ± 5.57 were found. Lying behaviour follows a seasonal progression with significant lowest LBD and highest FLB in summer. With the old AFS significant differences in LBD and FLB were detected between horned and hornless goats, but with the new AFS results were adjusted. Findings suggest that changes in feeding management do not affect the general seasonal progression of lying behaviour but can affect the behavioural differences between horned and hornless dairy goats.
Profile of Dogs’ Breeders and Their Considerations on Female Reproduction, Maternal Care and the Peripartum Stress—An International Survey
Authors: Santos Natalia Ribeiro , Beck Alexandra , Maenhoudt Cindy , Billy Charlotte , Fontbonne Alain
Published: August 2021

Dogs are part of many households worldwide and in recent years in some western countries, more and more people describe them as members of the family. The accurate number of dogs per household and the actual demand for puppies are hard to estimate correctly. The sources of dogs seem to be an important element to consider before acquiring a dog since some behavioural disorders are more likely observed in dogs coming from puppy mills and pet stores. Therefore, there is an increased need to better-know dog breeders, theirs believes and practices. To full-fill this objective, an online questionnaire in five different languages (English, German, Polish, Portuguese and Spanish) was sent to dog breeders. The survey intended to study the demographics of dog breeders and to understand their perception and practices about reproduction, maternal behaviour and management of the dam during the peripartum period. We were also interested to evaluate the occurrence and the impact of stress around parturition and how breeders deal with overstressed bitches and if they believe that motherly quality could have a later-on effect on the livelihood of the dog. Six hundred and sixty-eight respondents participated in the survey, mainly from Australia, Brazil, Canada, Germany, Portugal, Poland, Spain, United Kingdom, United States of America and non-listed country (reported as ‘other’ in the survey). A country effect was observed in relation to housing system, human interaction, the use of techniques to define breeding and whelping time and different methods to manage stress during the peripartum period. Considering the demographics of the responders, breeding activity is, in general, a family based activity with an effect of country. Use of progesterone levels to manage reproduction and pregnancy diagnosis by ultrasound were cited as common practice. In general, parturition takes place under human supervision. Peripartum period was perceived as a stressful moment with a difference in the way of addressing the problem based on the country but reassuring the bitch by increasing human presence was frequently described. Inappropriate maternal behaviour was observed by half of responders and if presented primiparous bitches and parturition by C-section were overrepresented. Puppies stayed with the dams from 4 weeks to 9 weeks and dog breeders from Spain, Poland and Portugal are keeping puppies with their mother the longest. Overall, poor maternal behaviour has an impact on puppies’ cognitive development with German breeders being more convinced than their counterparts from Brazil and Spain.
GIS as an Epidemiological Tool to Monitor the Spatial–Temporal Distribution of Tuberculosis in Large Game in a High-Risk Area in Portugal
Authors: Aranha José , Abrantes Ana Carolina , Gonçalves Raquel , Miranda Rui , Serejo João , Vieira-Pinto Madalena
Published: August 2021

Since April 2011, Portugal has implemented specific national legislation (Notice No. 1/2011), defining “Epidemiologic Risk Areas for Bovine Tuberculosis in Large Game” and mitigation measures in these areas, including Idanha-a-Nova county. A GIS project was created to record information that would allow us to analyse the spatial–temporal distribution, both for hunting bags and tuberculosis occurrence, in hunted wild boar and red deer in Idanha-a-Nova. Hunting bag and tuberculosis-like lesion data were recorded during post-mortem inspection across 11 hunting seasons, totalling 9844 animals. The difference in tuberculosis occurrence for these species was statistically significant in nearly all 11 seasons, with wild boars presenting approximately twice the occurrence of red deer. No significant difference was noted before and after the Notice No. 1/2011 implementation. These results, following GIS-based spatial analysis, enable us to state that both large game species displayed an irregular tuberculosis pattern for the 2006–2016 period, and we identified some specific areas of high risk for both species. Southern areas of the county may be considered the priority for intervention. This research demonstrates the potential of GIS tools to evaluate, in the field, the results and efficacy of legislation such as Notice No. 1/2011, and to ensure the correct implementation of cost-effective mitigation strategies for tuberculosis in large game species.
Exploring Information Available to and Used by Physicians on Antibiotic Use and Antibiotic Resistance in Jordan
Authors: Karasneh Reema A. , Al-Azzam Sayer I. , Ababneh Mera A. , Basheti Iman A. , Al-Azzeh Ola , Al Sharie Sarah , Conway Barbara R. , Aldeyab Mamoon A.
Published: August 2021

Evidence based information sources for physicians are needed for informed antibiotic prescribing practices. The aim of this study was to explore physicians’ preferred sources of information and evaluate physicians’ awareness of available information and initiatives on prudent antibiotic prescribing in Jordan. A cross-sectional study was conducted utilizing an online questionnaire and included physicians (n = 409) from all sectors and specialties in Jordan. Published guidelines (31.8%), the workplace (25.7%), colleagues or peers (20.0%), group or conference training (18.3%), and the medical professional body (18.1%) were the main sources of information about avoiding unnecessary antibiotic prescribing, with the influence of these sources on changing prescribers’ views being 34.7%, 17.1%, 11%, 13.4%, and 7.6%, respectively. One-third of physicians (33.7%) reported no knowledge of any initiatives on antibiotic awareness and resistance. Regarding awareness of national action plans on antimicrobial resistance, 10.5%, 34%, and 55.5% of physicians were aware, unaware, and unsure of the presence of any national action plans, respectively. Physicians showed interest in receiving more information on resistance to antibiotics (58.9%), how to use antibiotics (42.2%), medical conditions for which antibiotics are used (41.3%), prescribing of antibiotics (35.2%), and links between the health of humans, animals, and the environment (19.8%). The findings can inform interventions needed to design effective antimicrobial stewardship, enabling physicians to prescribe antibiotics appropriately.
Kidney Protection with the Radical Scavenger α1-Microglobulin (A1M) during Peptide Receptor Radionuclide and Radioligand Therapy
Authors: Kristiansson Amanda , Örbom Anders , Vilhelmsson Timmermand Oskar , Ahlstedt Jonas , Strand Sven-Erik , Åkerström Bo
Published: August 2021

α1-Microglobulin (A1M) is an antioxidant found in all vertebrates, including humans. It has enzymatic reductase activity and can scavenge radicals and bind free heme groups. Infused recombinant A1M accumulates in the kidneys and has therefore been successful in protecting kidney injuries in different animal models. In this review, we focus on A1M as a radioprotector of the kidneys during peptide receptor radionuclide/radioligand therapy (PRRT/RLT). Patients with, e.g., neuroendocrine tumors or castration resistant prostate cancer can be treated by administration of radiolabeled small molecules which target and therefore enable the irradiation and killing of cancer cells through specific receptor interaction. The treatment is not curative, and kidney toxicity has been reported as a side effect since the small, radiolabeled substances are retained and excreted through the kidneys. In recent studies, A1M was shown to have radioprotective effects on cell cultures as well as having a similar biodistribution as the somatostatin analogue peptide 177Lu-DOTATATE after intravenous infusion in mice. Therefore, several animal studies were conducted to investigate the in vivo radioprotective potential of A1M towards kidneys. The results of these studies demonstrated that A1M co-infusion yielded protection against kidney toxicity and improved overall survival in mouse models. Moreover, two different mouse studies reported that A1M did not interfere with tumor treatment itself. Here, we give an overview of radionuclide therapy, the A1M physiology and the results from the radioprotector studies of the protein.
A Literature Review of Energy Efficiency and Sustainability in Manufacturing Systems
Authors: Renna Paolo , Materi Sergio
Published: August 2021

Climate change mitigation, the goal of reducing CO2 emissions, more stringent regulations and the increment in energy costs have pushed researchers to study energy efficiency and renewable energy sources. Manufacturing systems are large energy consumers and are thus responsible for huge greenhouse gas emissions; for these reasons, many studies have focused on this topic recently. This review aims to summarize the most important papers on energy efficiency and renewable energy sources in manufacturing systems published in the last fifteen years. The works are grouped together, considering the system typology, i.e., manufacturing system subclasses (single machine, flow shop, job shop, etc.) or the assembly line, the developed energy-saving policies and the implementation of the renewable energy sources in the studied contexts. A description of the main approaches used in the analyzed papers was discussed. The conclusion reports the main findings of the review and suggests future directions for the researchers in the integration of renewable energy in the manufacturing systems consumption models.
Re-Engineering of an Impeller for Submersible Electric Pump to Be Produced by Selective Laser Melting
Authors: Ponticelli Gennaro Salvatore , Tagliaferri Flaviana , Venettacci Simone , Horn Matthias , Giannini Oliviero , Guarino Stefano
Published: August 2021

The subject of the present study is the reproduction of a submersible electric pump impeller through reverse engineering and additive manufacturing. All of the phases commonly envisaged in the reproduction of an existing piece were carried out. The aim of the study is to show how the chosen pump component can be effectively re-engineered and produced with the selective laser melting technique, obtaining a final product that is comparable if not even better than the starting one. To achieve this goal, the original piece was redesigned and a new model was created and analyzed. The whole process has been split into three main phases: (i) realization of the three-dimensional model from an existing piece using reverse engineering techniques; (ii) finite element analysis for the optimization of the use of the material; and (iii) 3D printing of a concept model in polyethylene terephthalate by using the fused deposition modeling technology and of the functional model in AISI 316 stainless steel with selective laser melting technology.
Characteristics of Enhanced Heatwaves over Tanzania and Scenario Projection in the 21st Century
Authors: Gyilbag Amatus , Amou Martial , Tulcan Roberto Xavier Supe , Zhang Lei , Demelash Tsedale , Xu Yinlong
Published: August 2021

Extreme hot temperature is dangerous to the bioeconomy, and would worsen with time. Ambient heatwaves accompanied by unusual droughts are major threats to poverty eradication in Tanzania. Due to sparsity of observation data and proper heatwave detection metrics, there has been a paucity of knowledge about heatwave events in Tanzania. In this study, the Heatwave Magnitude Index daily (HWMId) was adopted to quantitatively analyze heatwave characteristics throughout Tanzania at mid-21st century (2041–2070) and end of 21st century (2071–2100), relative to the reference period (1983–2012) using the CHIRTS-daily quasi-global high-resolution temperature dataset and climate simulations from a multi-modal ensemble of median scenarios (RCP4.5, from CORDEX-Africa). The results showed that moderate to super-extreme heatwaves occurred in Tanzania between 1983 and 2012, particularly in 1999, when ultra-extreme heatwaves (HWMId > 32) occurred in the Lake Victoria basin. It is projected that by mid-21st century, the upper category of HWMId would be hotter and longer, and would occur routinely in Tanzania. The spatial extent of all of the HWMId categories is projected to range from 34% to 73% by the end of the 21st century with a duration of 8 to 35 days, compared to 1 to 5 days during the reference period. These findings will contribute to increasing public awareness of the need for adaptation.
Meta-Analysis of Mitochondrial DNA Control Region Diversity to Shed Light on Phylogenetic Relationship and Demographic History of African Sheep (Ovis aries) Breeds
Authors: Wanjala George , Bagi Zoltán , Kusza Szilvia
Published: August 2021

To improve sheep breeding and conservation of genetic resources, the mitochondrial DNA control region (mtDNA CR) of 399 sequences of African indigenous sheep breeds from previously published research articles were meta-analyzed to elucidate their phylogenetic relationship, diversity, and demographic history. A total of 272 haplotypes were found, of which 207 were unique and a high level of mtDNA CR variability was observed. Generally, the number of polymorphic sites, nucleotide and haplotype diversity were high (284, 0.254 ± 0.012 and 0.993 ± 0.002, respectively). The median-joining (MJ) network of haplotypes produced three major haplogroups (A, B and C), with haplogroup B being dominant. A mixture of populations suggests a common matrilineal origin and lack of and/or a weak phylogeographic structure. Mismatch analysis showed recent expansion of North African breeds, whereas East African and continental populations exhibited selection pressures for adaptation. A slight historical genetic difference was also observed between the fat tail and thin tail sheep breeds. However, further investigations are required using more samples and long sequence segments to achieve deeper levels of conclusions on the African sheep phylogenetic relationship. The present meta-analysis results contribute to the general understanding of African native sheep populations for improved management of sheep genetic resources.
Candidate Glaucoma Biomarkers: From Proteins to Metabolites, and the Pitfalls to Clinical Applications
Authors: Fernández-Vega Cueto Andrés , Álvarez Lydia , García Montserrat , Álvarez-Barrios Ana , Artime Enol , Fernández-Vega Cueto Luis , Coca-Prados Miguel , González-Iglesias Héctor
Published: August 2021

Glaucoma is an insidious group of eye diseases causing degeneration of the optic nerve, progressive loss of vision, and irreversible blindness. The number of people affected by glaucoma is estimated at 80 million in 2021, with 3.5% prevalence in people aged 40–80. The main biomarker and risk factor for the onset and progression of glaucoma is the elevation of intraocular pressure. However, when glaucoma is diagnosed, the level of retinal ganglion cell death usually amounts to 30–40%; hence, the urgent need for its early diagnosis. Molecular biomarkers of glaucoma, from proteins to metabolites, may be helpful as indicators of pathogenic processes observed during the disease’s onset. The discovery of human glaucoma biomarkers is hampered by major limitations, including whether medications are influencing the expression of molecules in bodily fluids, or whether tests to validate glaucoma biomarker candidates should include human subjects with different types and stages of the disease, as well as patients with other ocular and neurodegenerative diseases. Moreover, the proper selection of the biofluid or tissue, as well as the analytical platform, should be mandatory. In this review, we have summarized current knowledge concerning proteomics- and metabolomics-based glaucoma biomarkers, with specificity to human eye tissue and fluid, as well the analytical approach and the main results obtained. The complex data published to date, which include at least 458 different molecules altered in human glaucoma, merit a new, integrative approach allowing for future diagnostic tests based on the absolute quantification of local and/or systemic biomarkers of glaucoma.
Enzymatic and Antimicrobial Activity of Biologically Active Samples from Aloe arborescens and Aloe barbadensis
Authors: Leitgeb Maja , Kupnik Kaja , Knez Željko , Primožič Mateja
Published: August 2021

Recently, the use of Aloe species has become very widespread. These are extensively used as a nutraceutical in a variety of health care products and food supplements. In addition, the occurrence of the quickly adaptable microorganisms, particularly bacteria, which can develop resistance to antibiotics, is a major problem for public health, and therefore, it is necessary to search for new antimicrobials. In our study, the content of total phenols, proanthocyanidins, and proteins in fresh and lyophilized samples of A. arborescens and A. barbadensis and their ethanol extracts was investigated. Furthermore, enzymatic and antioxidant activity of samples were studied. Since antimicrobial activity of fresh samples was determined in our latest research, a more detailed study of antimicrobial effectiveness of A. arborescens and A. barbadensis (lyophilized, extracts) was performed. Ethanol extracts in particular contain higher concentrations of bioactive substances and show the topmost antioxidant activity. The novelty of the study refers to the observation of industrially important enzyme activities such as α-amylase, cellulase, lipase, peroxidase, protease, and transglutaminase in the samples as well as the microbial growth inhibition rates determination (MGIR) at different concentrations of added aloe samples. All samples inhibited the growth of all tested microbial cells. MIC90 for A. arborescens and A. barbadensis were also determined in case of B. cereus, P. aeruginosa, P. fluorescens, and S. aureus. The results of our study tend to give credence to the popular use of both aloes in medicine and in the cosmetic, food, and pharmaceutical industries.