bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Skill-based Questions According to Science Education Teachers in Turkey
Authors:   Department of Educational Sciences, Faculty of Education, The University of Bartın, Bartın, Turkey, Yuzuak Ahmet Volkan , Recepkethüda Recep ,   Department of Educational Sciences, Ministry of National Education, Ankara, Turkey
Published: March 2022

This study aimed to determine science teachers’ opinions about the skills-based questions (SBQs) used in Turkey’s high school’s entrance exam. In this study, the phenomenology method was used. The study consisted of 15 participants working as science teachers with at least 5 years of professional experience in public schools in different provinces during the 2020–2021 academic years. The study data were collected through the semi-structured interview form prepared by the study’s researchers. The form included three questions related to the participants’ demographics and another 12 on SBQ. Content analysis was used by help of MAXQDA 20 to analyze the data. The study findings revealed that SBQ and lectures in textbooks were incompatible and books still offered traditional-type questions. Remarkably, science education is the most appropriate course to create SBQ. The teaching methods used in lectures should be associated with high-level thinking skills. Teachers need workshops related to SBQ. Therefore, this study has proposed that textbook contents be revised, and teachers be informed accordingly.
Impact of Research- and Assessment-based Instructional Modes on the Achievement of Senior High School Students in Selected Chemistry Topics
Authors:   Department of Educational Foundations, National Open University of Nigeria, Jabi, Abuja, Opateye Johnson Ayodele , Ewim Daniel Raphael Ejike ,   Department of Mechanical Engineering, Durban University of Technology, Durban, South Africa
Published: March 2022

This research focused on the impact of research- and assessment-based instruction on senior high school students’ achievement in selected chemistry topics. A 3 × 2 × 2 pre-test and post-test control group in a quasi-experimental research design was used on a population of Grade 11 chemistry students in Ojo Local Government Area of Lagos state. Two schools (one public and one private) were randomly assigned to each of the treatments. A sample of 240 chemistry students was used. Three research questions were answered and five hypotheses were tested. The reliability coefficient of the “Chemistry achievement test” was found to be 0.770 using the Kuder–Richardson Formula 20. Research-based, assessment-based, and conventional instruction manuals were also developed. The data collected were analyzed using descriptive statistics and analysis of covariance. It was found that the impact of assessment- and research-based instruction on senior high school students’ achievement in the selected topics was significant (F (2,227) = 49.214, p < 0.050). It was also found that the students exposed to the assessment-based instructional mode had a higher achievement than those in the research-based and conventional groups. Male chemistry students were found to benefit more than the female students after being exposed to research- and assessment-based instructional modes. It is, therefore, recommended that assessment- and research-based instructional modes be used to teach the selected chemistry topics to enhance effective learning.
Hubungan Kejadian Stunting dengan Perkembangan Anak Usia 6-23 Bulan
Authors: Syahruddin Akmal Novrian , Ningsih Nining Ade , Menge Fransiska
Published: March 2022

Stunting masih menjadi masalah kesehatan masyarakat yang serius di Indonesia yang berdampak tidak hanya pada pertumbuhan linear tetapi juga pada perkembangan anak.  Penelitian ini bertujuan untuk mengetahui hubungan kejadian stunting dengan perkembangan anak usia 6-23 bulan di Wilayah Kerja Puskemas Taraweang Kecamatan Labakkang Kabupaten Pangkep. Penelitian ini merupakan kuantitatif dengan pendekatan cross sectional study. Sampel penelitian sebanyak 72 responden berusia 6-23 bulan yang ditentukan dengan metode proportional random sampling. Stunting diukur berdasarkan nilai Z-score Panjang Badan berdasarkan Umur (PB/U) kurang dari -2 SD dan pengukuran perkembangan menggunakan kuesioner pra skining perkembangan (KPSP). Data dianalisis secara univariat dan bivariat menggunakan uji Chi-Square. Hasil penelitian menunjukkan bahwa kejadian stunting sebesar 36,1%. Stunting lebih banyak pada laki-laki (38,5%) dan meningkat sesuai kategori pertambahan umur 6-11, 12-17 dan 18-23 bulan berturut turut  25.0%, 36,4% dan 46.2%.  Perkembangan anak dalam kategori meragukan sebesar 40,3%. Berdasarkan analisis menunjukkan hubungan antara kejadian stunting dengan perkembangan anak dengan nilai p-value 0.012 (p<0,05). Kesimpulan dari penelitian ini adalah stunting berhubungan dengan hambatan perkembangan anak usia 6-23 bulan di wilayah kerja Puskesmas Taraweang, Kabupaten Pangkep
Effects of Supplemental Leucine on Growth, Nutrient Use, and Muscle and Visceral Tissue Mass in Holstein Bull Calves Fed Milk Replacer
Authors: Reiners Jessica N. , Steele Michael A. , Vonnahme Kimberly A. , Maddock Carlin Kasey R. , Swanson Kendall C.
Published: March 2022

To determine the effects of leucine supplementation on body weight (BW), tissue mass, nutrient digestibility, the concentration of serum amino acids (AAs) and metabolites, and protein abundance of elongation initiation factor 4E (eIF4E) in skeletal muscle, 23 Holstein bull calves (43. 3 ± 1.16 kg; 11.3 ± 0.57 days of age) fed milk replacer at 2.5% of body weight (BW; dry matter basis) were used in a randomized complete block design. Leucine was supplemented at 0, 0.4, 0.6, or 0.8 g Leu/kg BW per day for 28 d. Data were analyzed using the MIXED procedure of SAS. Leucine supplementation did not affect calf BW (P = 0.73), and digestibility of nitrogen (P = 0.21), organic matter (P = 0.28), and dry matter (P = 0.28). Masses proportional to BW of the pancreas (P = 0.04), omasum (P < 0.01), and spleen (P = 0.01) were quadratically affected by treatment where tissue mass decreased at 0.4 g Leu/kg BW and increased at 0.6 and 0.8 g Leu/kg BW. Semitendinosus mass proportional to BW tended (P = 0.07) to be quadratically affected, as tissue mass increased at 0.4 g Leu/kg BW, and decreased at 0.6 and 0.8 g Leu/kg BW. Serum Leu concentration increased linearly (P = 0.002; day × time × treatment) across days and after feedings with increased supplemental Leu. Increasing supplemental Leu linearly decreased serum Ala (P < 0.01), Arg (P = 0.04), Ile (P = 0.02), Met (P < 0.01), and Pro (P = 0.05) concentrations, and quadratically affected serum Glu (P = 0.04) and Lys (P = 0.03) concentrations where serum Glu and Lys concentrations were decreased at 0.4 g Leu/kg BW and increased at 0.6 and 0.8 g Leu/kg BW. There was no effect of treatment on protein abundance of eIF4E in semitendinosus or longissimus dorsi. These data indicate that supplemental Leu did not influence ADG and nitrogen retention in calves fed milk replacer. However, changes in serum AA concentrations and tissue masses proportional to BW suggest that supplementation of Leu at lower levels could increase the use of AA for non-visceral tissue growth.
Proteomic Responses of Dark-Adapted Euglena gracilis and Bleached Mutant Against Light Stimuli
Authors: Chen Zhenfan , Chen Zixi , Zhu Jiayi , He Jiayi , Liu Qiong , Zhu Hui , Lei Anping , Wang Jiangxin
Published: March 2022

Euglena gracilis (E. gracilis) has secondary endosymbiotic chloroplasts derived from ancient green algae. Its chloroplasts are easily lost under numerous conditions to become permanently bleached mutants. Green cells adapted in the dark contain undeveloped proplastids and they will develop into mature chloroplasts after 3 days of light exposure. Thus, E. gracilis is an ideal model species for a chloroplast development study. Previous studies about chloroplast development in E. gracilis focused on morphology and physiology, whereas few studies have addressed the regulatory processes induced by light in the proteome. In this study, the whole-genome proteome of dark-adapted E. gracilis (WT) and permanently ofloxacin-bleached mutant (B2) was compared under the light exposure after 0, 12, and 72 h. The results showed that the photosynthesis-related proteins were up-regulated over time in both WT and B2. The B2 strain, with losing functional chloroplasts, seemed to possess a complete photosynthetic function system. Both WT and B2 exhibited significant light responses with similar alternation patterns, suggesting the sensitive responses to light in proteomic levels. The main metabolic activities for the utilization of carbon and energy in WT were up-regulated, while the proteins with calcium ion binding, cell cycle, and non-photosynthetic carbon fixation were down-regulated in B2. This study confirmed light-induced chloroplast development in WT from dark, and also for the first time investigates the light responses of a bleached mutant B2, providing more information about the unknown functions of residual plastids in Euglena bleached mutants.
The Collagen Receptor Discoidin Domain Receptor 1b Enhances Integrin β1-Mediated Cell Migration by Interacting With Talin and Promoting Rac1 Activation
Authors: Borza Corina M. , Bolas Gema , Zhang Xiuqi , Browning Monroe Mary Beth , Zhang Ming-Zhi , Meiler Jens , Skwark Marcin J. , Harris Raymond C. , Lapierre Lynne A. , Goldenring James R. , Hook Magnus , Rivera Jose , Brown Kyle L. , Leitinger Birgit , Tyska Matthew J. , Moser Markus , Böttcher Ralph T. , Zent Roy , Pozzi Ambra
Published: March 2022

Integrins and discoidin domain receptors (DDRs) 1 and 2 promote cell adhesion and migration on both fibrillar and non fibrillar collagens. Collagen I contains DDR and integrin selective binding motifs; however, the relative contribution of these two receptors in regulating cell migration is unclear. DDR1 has five isoforms (DDR1a-e), with most cells expressing the DDR1a and DDR1b isoforms. We show that human embryonic kidney 293 cells expressing DDR1b migrate more than DDR1a expressing cells on DDR selective substrata as well as on collagen I in vitro. In addition, DDR1b expressing cells show increased lung colonization after tail vein injection in nude mice. DDR1a and DDR1b differ from each other by an extra 37 amino acids in the DDR1b cytoplasmic domain. Interestingly, these 37 amino acids contain an NPxY motif which is a central control module within the cytoplasmic domain of β integrins and acts by binding scaffold proteins, including talin. Using purified recombinant DDR1 cytoplasmic tail proteins, we show that DDR1b directly binds talin with higher affinity than DDR1a. In cells, DDR1b, but not DDR1a, colocalizes with talin and integrin β1 to focal adhesions and enhances integrin β1-mediated cell migration. Moreover, we show that DDR1b promotes cell migration by enhancing Rac1 activation. Mechanistically DDR1b interacts with the GTPase-activating protein (GAP) Breakpoint cluster region protein (BCR) thus reducing its GAP activity and enhancing Rac activation. Our study identifies DDR1b as a major driver of cell migration and talin and BCR as key players in the interplay between integrins and DDR1b in regulating cell migration.
Lipid Analysis of Fracture Hematoma With MALDI-MSI: Specific Lipids are Associated to Bone Fracture Healing Over Time
Authors: Groven Rald V. M. , Nauta Sylvia P. , Gruisen Jane , Claes Britt S. R. , Greven Johannes , van Griensven Martijn , Poeze Martijn , Heeren Ron M. A. , Porta Siegel Tiffany , Cillero-Pastor Berta , Blokhuis Taco J.
Published: March 2022

Background: Fracture healing is a complex process, involving cell-cell interactions, various cytokines, and growth factors. Although fracture treatment improved over the last decades, a substantial part of all fractures shows delayed or absent healing. The fracture hematoma (fxh) is known to have a relevant role in this process, while the exact mechanisms by which it influences fracture healing are poorly understood. To improve strategies in fracture treatment, regulatory pathways in fracture healing need to be investigated. Lipids are important molecules in cellular signaling, inflammation, and metabolism, as well as key structural components of the cell. Analysis of the lipid spectrum in fxh may therefore reflect important events during the early healing phase. This study aims to develop a protocol for the determination of lipid signals over time, and the identification of lipids that contribute to these signals, with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in fxh in healthy fracture healing.

Methods: Twelve fxh samples (6 porcine; 6 human) were surgically removed, snap frozen, sectioned, washed, and analyzed using MALDI-MSI in positive and negative ion mode at different time points after fracture (porcine: 72 h; human samples: range 1–19 days). A tissue preparation protocol for lipid analysis in fxh has been developed with both porcine and human fxh. Data were analyzed through principal component- and linear discriminant analyses.

Results: A protocol for the preparation of fxh sections was developed and optimized. Although hematoma is a heterogeneous tissue, the intra-variability within fxh was smaller than the inter-variability between fxh. Distinctive m/z values were detected that contributed to the separation of three different fxh age groups: early (1–3 days), middle (6–10 days), and late (12–19 days). Identification of the distinctive m/z values provided a panel of specific lipids that showed a time dependent expression within fxh.

Conclusion: This study shows that MALDI-MSI is a suitable analytical tool for lipid analysis in fxh and that lipid patterns within fxh are time-dependent. These lipid patterns within fxh may serve as a future diagnostic tool. These findings warrant further research into fxh analysis using MALDI-MSI and its possible clinical implications in fracture treatment.
Ultrasensitive Immunosensor for Prostate-Specific Antigen Based on Enhanced Electrochemiluminescence by Vertically Ordered Mesoporous Silica-Nanochannel Film
Authors: Ma Kai , Zheng Yanyan , An Lizhe , Liu Jiyang
Published: March 2022

Ultrasensitive and specific detection of prostate-specific antigen (PSA) in complex biological samples is crucial for early diagnosis and treatment of prostate-related diseases. Immunoassay with a simple sensing interface and ultrahigh sensitivity is highly desirable. Herein, a novel electroluminescence (ECL) immunosensing platform is demonstrated based on the equipment of vertically ordered mesoporous silica-nanochannel films (VMSFs) with PSA antibody, which is able to realize ultrasensitive detection of PSA in human serum. Through the electrochemically assisted self-assembly (EASA) method, the VMSF is easily grown on an indium tin oxide (ITO) electrode in a few seconds. Owing to a large surface area and the negatively charged surface, VMSF nanochannels display strong electrostatic attraction to the positively charged ECL luminophores (tris(2,2-bipyridyl) dichlororuthenium (II), (Ru(bpy)32+), leading to two orders-of-magnitude enhancement of ECL emission compared with that of the bare ITO electrode. The outer surface of the VMSF is functionalized with reactive epoxy groups, which further allows covalent attachment of PSA antibody (Ab) on the entry of nanochannels. As the combination of PSA with Ab decreases the ECL signal by hindering the mass transfer of ECL luminophores and coreactant, the developed immunosensor can achieve ultrasensitive detection of PSA ranging from 1 pg ml−1 to 100 ng ml−1 with a limit of detection (LOD) of 0.1 pg ml−1. Considering the antifouling ability of the VMSF, sensitive detection of PSA in human serum is also realized. The proposed nanochannel-based immunosensor may open up a new way for the facile development of the universal immunosensing platform for rapid and ultrasensitive detection of disease markers.
Development of an Adeno-Associated Virus-Vectored SARS-CoV-2 Vaccine and Its Immunogenicity in Mice
Authors: Qin Xi , Li Shanhu , Li Xiang , Pei Dening , Liu Yu , Ding Youxue , Liu Lan , Bi Hua , Shi Xinchang , Guo Ying , Fang Enyue , Huang Fang , Yu Lei , Zhu Liuqiang , An Yifang , Valencia C. Alexander , Li Yuhua , Dong Biao , Zhou Yong
Published: March 2022

Owing to the outbreak of the novel coronavirus (SARS-CoV-2) worldwide at the end of 2019, the development of a SARS-CoV-2 vaccine became an urgent need. In this study, we developed a type 9 adeno-associated virus vectored vaccine candidate expressing a dimeric receptor binding domain (RBD) of the SARS-CoV-2 spike protein (S protein) and evaluated its immunogenicity in a murine model. The vaccine candidate, named AAV9-RBD virus, was constructed by inserting a signal peptide to the N-terminus of two copies of RBD, spaced by a linker, into the genome of a type 9 adeno-associated virus. In vitro assays showed that HeLa cells infected by the recombinant AAV virus expressed high levels of the recombinant RBD protein, mostly found in the cell culture supernatant. The recombinant AAV9-RBD virus was cultured and purified. The genome titer of the purified recombinant AAV9-RBD virus was determined to be 2.4 × 1013 genome copies/mL (GC/mL) by Q-PCR. Balb/c mice were immunized with the virus by intramuscular injection or nasal drip administration. Eight weeks after immunization, neutralizing antibodies against the new coronavirus pseudovirus were detected in the sera of all mice; the mean neutralizing antibody EC50 values were 517.7 ± 292.1 (n=10) and 682.8 ± 454.0 (n=10) in the intramuscular injection group and nasal drip group, respectively. The results of this study showed that the recombinant AAV9-RBD virus may be used for the development of a SARS-CoV-2 vaccine.
Gut Microbiome Dysbiosis in Alcoholism: Consequences for Health and Recovery
Authors: Day Andrew Whittier , Kumamoto Carol A.
Published: March 2022

Since the mid 1980’s, the impact of gastrointestinal (GI) microbiome changes during alcohol use disorder has been an area of significant interest. This work has resulted in the identification of specific changes in the abundance of certain members of the GI microbiome and the role these changes play in a variety of alcohol related disorders (i.e. alcoholic liver disease). Interestingly, some findings suggest a possible role for the GI microbiome in alcohol addiction or withdrawal. Unfortunately, there is a significant gap in knowledge in this area. Here we describe differences in the GI microbiome of alcoholic and non-alcoholic individuals and discuss the possible impact of microbes on the gut-brain axis, which could impact alcohol related behaviors (i.e. addiction). Understanding the role of the GI microbiome in alcohol related disorders will potentially lead to the development of successful microbiome-targeted therapeutics to help mitigate these disorders.
Metagenomic Assessment of the Pathogenic Risk of Microorganisms in Sputum of Postoperative Patients With Pulmonary Infection
Authors: Chen Junji , Sun Lianjie , Liu Xiaoying , Yu Qixiang , Qin Kaijie , Cao Xuejie , Gu Jianwei
Published: March 2022

Respiratory infections are complicated biological processes associated with an unbalanced microbial community and a wide range of pathogens. To date, robust approaches are still required for distinguishing the pathogenic microorganisms from the colonizing ones in the clinical specimens with complex infection. In this study, we retrospectively analyzed the data of conventional culture testing and metagenomic next-generation sequencing (mNGS) of the sputum samples collected from 50 pulmonary infected patients after cardiac surgery from December 2020 and June 2021 in Ruijin Hospital. Taxonomic classification of the sputum metagenomes showed that the numbers of species belonging to bacteria, fungi, and viruses were 682, 58, and 21, respectively. The full spectrum of microorganisms present in the sputum microbiome covered all the species identified by culture, including 12 bacterial species and two fungal species. Based on species-level microbiome profiling, a reference catalog of microbial abundance detection limits was constructed to assess the pathogenic risks of individual microorganisms in the specimens. The proposed screening procedure detected 64 bacterial pathogens, 10 fungal pathogens, and three viruses. In particular, certain opportunistic pathogenic strains can be distinguished from the colonizing ones in the individual specimens. Strain-level identification and phylogenetic analysis were further performed to decipher molecular epidemiological characteristics of four opportunistic etiologic agents, including Klebsiella pneumoniae, Corynebacterium striatum, Staphylococcus aureus, and Candida albicans. Our findings provide a novel metagenomic insight into precision diagnosis for clinically relevant microbes, especially for opportunistic pathogens in the clinical setting.
“Depends on Who I'm Writing To”—The Influence of Addressees and Personality Traits on the Use of Emoji and Emoticons, and Related Implications for Forensic Authorship Analysis
Authors: Marko Karoline
Published: March 2022

Converging towards or diverging from an interlocutor's speaking style (i.e., linguistic accommodation) has been investigated in many previous studies and is a highly relevant phenomenon in forensic authorship analysis. Accommodation has also been linked to personality traits, but there is still a lack of investigations of accommodation in computer-mediated communication. The present study thus aims at examining in how far emoji use is subject to accommodation in casual online interactions, and how reliable both emoji and emoticons are as markers of authorship. Further, this study is interested in finding out about connections between both emoji and emoticon use and the Big Five personality traits of agreeableness, emotional stability, conscientiousness, openness to experiences, and extraversion. The results of the analysis show that the frequency of emoji use is indeed strongly influenced by conversation partners, and that both emoji and emoticon use correlate particularly with extraversion and agreeableness. Despite the influence of conversation partners on emoji use, it can further be shown that emoji and emoticons remain valuable markers of authorship to different degrees.
Association Between Extraversion Personality With the Blood Pressure Level in Adolescents
Authors: Liang Xiaohua , Hao Guang , Xiao Lun , Luo Shunqing , Zhang Guifang , Tang Xian , Qu Ping , Li Rina
Published: March 2022

Purpose

The association between extraversion personality and high blood pressure (BP) has not been well-studied. This study aims to explore the association between extraversion personality and the BP level in a large sample of adolescents.

Methods

As an ongoing study, 5,246 children aged 6–9 years were recruited using a stratified cluster sampling method in 2014. The extraversion personality trait, assessed by the Eysenck personality questionnaire (EPQ, answered by adolescents) in 2019, was used in the main analysis. A total of 3,407 participants were eligible and included in this analysis.

Results

The EPQ extraversion score was negatively associated with a systolic BP, a diastolic BP, and mean arterial pressure (MAP) in a univariate analysis. After adjusting for other covariates, high extraversion score was negatively associated with systolic BP (β = −0.026; 95% CI = −0.047, −0.004; p = 0.002). There was an interaction between regions and the extraversion score on systolic BP (Pinteraction = 0.037). The stratified analysis showed that, in rural areas, the extraversion score was negatively associated with systolic BP (β = −0.050; 95% CI = −0.081, −0.016; p = 0.004). However, we did find an association between the extraversion score and systolic BP in urban areas (β = 0.000; 95% CI = −0.028, 0.028; p = 0.996). Similar results were observed for the extraversion assessed by parents.

Conclusions

We observed that extraversion personality was negatively associated with high BP in adolescents, especially for those who were living in rural areas. Our results suggested that a comprehensive intervention should be implemented to promote psychological health in adolescents.
Plasma Big Endothelin-1 Levels and Long-Term Outcomes in Patients With Atrial Fibrillation and Acute Coronary Syndrome or Undergoing Percutaneous Coronary Intervention
Authors: Lyu Si-qi , Zhu Jun , Wang Juan , Wu Shuang , Zhang Han , Shao Xing-hui , Yang Yan-min
Published: March 2022

Background

This study aimed to evaluate the association between plasma big ET-1 levels and long-term outcomes in patients with atrial fibrillation (AF) and acute coronary syndrome (ACS) or undergoing percutaneous coronary intervention (PCI).

Methods

A total of 930 patients were enrolled and followed up for a median duration of 2.3 years. According to the optimal cutoff of big ET-1 for predicting all-cause death, these patients were divided into two groups. The primary endpoints were all-cause death and net adverse clinical events (NACE). The secondary endpoints included cardiovascular death, major adverse cardiovascular events (MACE), BARC class ≥ 3 bleeding, and BARC class ≥ 2 bleeding. Cox regressions were performed to evaluate the association between big ET-1 and outcomes.

Results

Based on the optimal cutoff of 0.54 pmol/l, 309 patients (33.2%) had high big ET-1 levels at baseline. Compared to the low big ET-1 group, patients in the high big ET-1 group tended to have more comorbidities, impaired cardiac function, elevated inflammatory levels, and worse prognosis. Univariable and multivariable Cox regressions indicated that big ET-1 ≥ 0.54 pmol/l was associated with increased incidences of all-cause death [HR (95%CI):1.73 (1.10–2.71), p = 0.018], NACE [HR (95%CI):1.63 (1.23–2.16), p = 0.001], cardiovascular death [HR (95%CI):1.72 (1.01–2.92), p = 0.046], MACE [HR (95%CI):1.60 (1.19–2.16), p = 0.002], BARC class ≥ 3 [HR (95%CI):2.21 (1.16–4.22), p = 0.016], and BARC class ≥ 2 bleeding [HR (95%CI):1.91 (1.36–2.70), p < 0.001]. Subgroup analysis indicated consistent relationships between the big ET-1 ≥ 0.54 pmol/l and the primary endpoints.

Conclusion

Elevated plasma big ET-1 levels were independently associated with increased risk of all-cause death, NACE, cardiovascular death, MACE, BARC class ≥ 3 bleeding, and BARC class ≥ 2 bleeding in patients with AF and ACS or undergoing PCI.
The Technological Basis of a Balloon-Expandable TAVR System: Non-occlusive Deployment, Anchorage in the Absence of Calcification and Polymer Leaflets
Authors: Appa Harish , Park Kenneth , Bezuidenhout Deon , van Breda Braden , de Jongh Bruce , de Villiers Jandré , Chacko Reno , Scherman Jacques , Ofoegbu Chima , Swanevelder Justiaan , Cousins Michael , Human Paul , Smith Robin , Vogt Ferdinand , Podesser Bruno K. , Schmitz Christoph , Conradi Lenard , Treede Hendrik , Schröfel Holger , Fischlein Theodor , Grabenwöger Martin , Luo Xinjin , Coombes Heather , Matskeplishvili Simon , Williams David F. , Zilla Peter
Published: March 2022

Leaflet durability and costs restrict contemporary trans-catheter aortic valve replacement (TAVR) largely to elderly patients in affluent countries. TAVR that are easily deployable, avoid secondary procedures and are also suitable for younger patients and non-calcific aortic regurgitation (AR) would significantly expand their global reach. Recognizing the reduced need for post-implantation pacemakers in balloon-expandable (BE) TAVR and the recent advances with potentially superior leaflet materials, a trans-catheter BE-system was developed that allows tactile, non-occlusive deployment without rapid pacing, direct attachment of both bioprosthetic and polymer leaflets onto a shape-stabilized scallop and anchorage achieved by plastic deformation even in the absence of calcification. Three sizes were developed from nickel-cobalt-chromium MP35N alloy tubes: Small/23 mm, Medium/26 mm and Large/29 mm. Crimp-diameters of valves with both bioprosthetic (sandwich-crosslinked decellularized pericardium) and polymer leaflets (triblock polyurethane combining siloxane and carbonate segments) match those of modern clinically used BE TAVR. Balloon expansion favors the wing-structures of the stent thereby creating supra-annular anchors whose diameter exceeds the outer diameter at the waist level by a quarter. In the pulse duplicator, polymer and bioprosthetic TAVR showed equivalent fluid dynamics with excellent EOA, pressure gradients and regurgitation volumes. Post-deployment fatigue resistance surpassed ISO requirements. The radial force of the helical deployment balloon at different filling pressures resulted in a fully developed anchorage profile of the valves from two thirds of their maximum deployment diameter onwards. By combining a unique balloon-expandable TAVR system that also caters for non-calcific AR with polymer leaflets, a powerful, potentially disruptive technology for heart valve disease has been incorporated into a TAVR that addresses global needs. While fulfilling key prerequisites for expanding the scope of TAVR to the vast number of patients of low- to middle income countries living with rheumatic heart disease the system may eventually also bring hope to patients of high-income countries presently excluded from TAVR for being too young.
Concurrent Primary Aldosteronism and Renal Artery Stenosis: An Overlooked Condition Inducing Resistant Hypertension
Authors: Zhao Lin , Xue Jinhong , Zhou Yi , Dong Xueqi , Luo Fang , Jiang Xiongjing , Du Xinping , Zhou Xianliang , Meng Xu
Published: March 2022

To explore the clinical features of coexisting primary aldosteronism (PA) and renal artery stenosis (RAS), we retrospectively analyzed records from 71 patients with PA with RAS and a control group of 121 patients with PA without RAS. Aldosterone-to-renin concentration ratio tests and computerized tomography (CT) scanning of the adrenal and renal arteries were routinely conducted to screen for PA and RAS. Color Doppler flow and/or magnetic resonance imaging were used as substitute testing of patients for whom CT was contraindicated. Standard percutaneous renal arteriography (PTRA) was considered for patients with RAS exceeding 70% based on non-invasive tests and for those without PTRA contraindications. The patients with PA with RAS were further divided into severe (RAS>70%) and moderate (50% < RAS <70%) RAS groups. The prevalence of RAS among PA patients was 6.9% (71/1,033), including 3.2% (33/1,033) with severe RAS. Compared with the PA without RAS group, the severe RAS group showed higher levels of systolic blood pressure (SBP) (171.82 ± 18.24 vs. 154.11 ± 18.96 mmHg; P < 0.001) and diastolic BP(DBP) (110.76 ± 15.90 vs. 91.73 ± 12.85 mmHg; P < 0.001) and prevalence of resistant hypertension (RH) (90.9 vs. 66.9%; P = 0.008), whereas the moderate RAS group merely showed higher DBP (98.63 ± 14.90 vs. 91.73 ± 12.85 mmHg; P = 0.006). The direct renin concentrations (DRCs) (5.37 ± 3.94 vs. 3.71 ± 2.10 μU/mL; P < 0.001) and false-negative rate (33.8 vs. 3.3%; P < 0.01) of PA screening tests were significantly higher in the PA with RAS group than in the control group, but only in severe RAS group, in subgroup analysis. Among patients who underwent successful treatment for severe RAS, mean DRC decreased from 11.22 ± 9.10 to 3.24 ± 2.69 μIU/mL (P < 0.001). Overall, the prevalence of RH decreased from 81.7 to 2.8% (P < 0.001) when both PA and RAS were treated with standard methods. PA with concurrent severe RAS is a condition that induces RH. PA can be easily missed in patients with coexisting RAS. RAS patients with RH after successful revascularization for RAS should be evaluated for coexisting PA.
A High Triglyceride-Glucose Index Value Is Associated With an Increased Risk of Carotid Plaque Burden in Subjects With Prediabetes and New-Onset Type 2 Diabetes: A Real-World Study
Authors: Jiang Zhen-zhen , Zhu Jian-bo , Shen Hua-liang , Zhao Shan-shan , Tang Yun-yi , Tang Shao-qi , Liu Xia-tian , Jiang Tian-an
Published: March 2022

Background

The triglyceride-glucose (TyG) index has been proposed as a convincing indicator of insulin resistance and has been found to be associated with atherosclerosis among diabetic patients. However, the relationship between the TyG index and arteriosclerosis in subjects with prediabetes and new-onset type 2 diabetes (T2D) remains uncertain. The purpose of this study was to assess the degree of carotid plaque burden in patients with prediabetes and new-onset T2D and to investigate the association between the TyG index and the degree of carotid plaque burden in this population.

Methods

This was a cross-sectional observational study that included 716 subjects aged 40–70 years old with prediabetes or new-onset T2D. Demographic, anthropometric, and laboratory measurements were collected. Participants underwent carotid arteriosclerosis evaluation by ultrasonography, and the degree of atherosclerosis was evaluated according to the carotid plaque burden. The TyG index was calculated.

Results

The population was stratified into high or low TyG index groups according to the median TyG index value. Higher values were associated with a higher BMI and waist circumference as well as higher total cholesterol, triglyceride, low-density lipoprotein cholesterol, plasma glucose, glycated hemoglobin, fasting C-peptide, and C-reactive protein levels (P < 0.001). The high TyG index group had a higher atherosclerotic plaque burden than the low TyG index group (P < 0.001). Multiclassification logistic regression analysis showed that the TyG index was positively associated with a high plaque burden [odds ratio (OR): 16.706, 95% confidence interval (CI): 3.988–69.978, P = 0.000], while no association was found between the TyG index and a low/moderate plaque burden. This association remained consistent in the subgroup analysis. In multiple linear regression analysis, sex, age, and the TyG index were found to be independently associated with carotid plaque burden. For each unit increase in the TyG index, the risk of a high carotid plaque burden increased 1.595-fold.

Conclusion

A high TyG index was positively associated with a high carotid plaque burden in subjects with prediabetes and new-onset T2D. Clinicians should pay close attention to the TyG index to help these patients receive the greatest benefit from early intervention.
Restitution Slope Affects the Outcome of Dominant Frequency Ablation in Persistent Atrial Fibrillation: CUVIA-AF2 Post-Hoc Analysis Based on Computational Modeling Study
Authors: Park Je-Wook , Lim Byounghyun , Hwang Inseok , Kwon Oh-Seok , Yu Hee Tae , Kim Tae-Hoon , Uhm Jae-Sun , Joung Boyoung , Lee Moon-Hyoung , Pak Hui-Nam
Published: March 2022

Introduction

Although the dominant frequency (DF) localizes the reentrant drivers and the maximal slope of the action potential duration (APD) restitution curve (Smax) reflects the tendency of the wave-break, their interaction has never been studied. We hypothesized that DF ablation has different effects on atrial fibrillation (AF) depending on Smax.

Methods

We studied the DF and Smax in 25 realistic human persistent AF model samples (68% male, 60 ± 10 years old). Virtual AF was induced by ramp pacing measuring Smax, followed by spatiotemporal DF evaluation for 34 s. We assessed the DF ablation effect depending on Smax in both computational modeling and a previous clinical trial, CUVIA-AF (170 patients with persistent AF, 70.6% male, 60 ± 11 years old).

Results

Mean DF had an inverse relationship with Smax regardless of AF acquisition timing (p < 0.001). Virtual DF ablations increased the defragmentation rate compared to pulmonary vein isolation (PVI) alone (p = 0.015), especially at Smax <1 (61.5 vs. 7.7%, p = 0.011). In post-DF ablation defragmentation episodes, DF was significantly higher (p = 0.002), and Smax was lower (p = 0.003) than in episodes without defragmentation. In the post-hoc analysis of CUVIA-AF2, we replicated the inverse relationship between Smax and DF (r = −0.47, p < 0.001), and we observed better rhythm outcomes of clinical DF ablations in addition to a PVI than of empirical PVI at Smax <1 [hazard ratio 0.45, 95% CI (0.22–0.89), p = 0.022; log-rank p = 0.021] but not at ≥ 1 (log-rank p = 0.177).

Conclusion

We found an inverse relationship between DF and Smax and the outcome of DF ablation after PVI was superior at the condition with Smax <1 in both in-silico and clinical trials.
Mini-Invasive Bentall Procedure Performed via a Right Anterior Thoracotomy Approach With a Costochondral Cartilage Sparing
Authors: Ji Qiang , Wang YuLin , Liu FangYu , Yang Ye , Li Jun , Sun XiaoNing , Yang ZhaoHua , Pan Sun , Lai Hao , Wang ChunSheng
Published: March 2022

Objectives

A right minithoracotomy approach with a sternal sparing technique is a minimally invasive option for surgeons performing aortic root surgery. This report presents our initial clinical results of the right minithoracotomy Bentall procedure.

Methods

Clinical data of 15 patients were retrospectively analyzed who underwent the minimally invasive Bentall procedure through the right anterior thoracotomy via the second intercostal incision without any costochondral cartilage invasion at our institution between October, 2019 and June, 2021. The operative time, length of intensive care unit stay and postoperative hospital stay, perioperative outcomes, and follow-up results were analyzed.

Results

The median aortic cross-clamping time was 95.0 (85.5–98.8) min. Three (21.4%) patients received blood transfusion. The median drainage volume in the first 24 h was 200.0 ml, with no redo for bleeding. The median duration of mechanical ventilation was 12.5 (11.0–25.0) h, and median length of intensive care unit stay was 1.5 (1.0–3.0) day. All patients discharged 5.8 ± 1.2 days following surgery, with no dead patients found. At 6 months following surgery, all patients survived with an improved New York Heart Association (NYHA) functional class.

Conclusion

The right minithoracotomy Bentall procedure may be performed safely with low morbidity and mortality. This approach should be considered as an option in carefully selected patients requiring aortic root replacement.
Dissolved Nitrogen Speciation and Concentration During Spring Thaw in the Greenland Ice Sheet Dark Zone: Evidence for Microbial Activity
Authors: Holland Alexandra T. , Williamson Christopher J. , Tedstone Andrew J. , Anesio Alexandre M. , Tranter Martyn
Published: March 2022

This study provides the first contemporaneous measurements of the concentration and speciation of dissolved nitrogen (N) in snow, meltwater and ice during the onset of the ablation season at a site within the Dark Zone of the Greenland Ice Sheet. The decaying, partially leached snow pack near S6 in south-west Greenland produced meltwater with relatively constant nitrate (NO3−) concentrations, approximating the snow pack average (1.1 µM). By contrast, ammonium (NH4+) (0–∼ 4 µM) and dissolved organic nitrogen (DON) (0–∼ 3 µM) concentrations were more variable, and sometimes higher than the average snow pack concentrations of 1.0 and 0.4 µM, respectively. This variability could be the result of microbial uptake and production within the melting snow pack. We observed pooled meltwater at the snow-ice interface that appeared to scavenge DON (∼1–8 µM) and possibly NO3− (∼1–2 µM) from the underlying ice, whose initial surface was a continuum of superimposed ice and weathering crust from the previous season. The shallow meteoric ice (∼10 cm–1 m) beneath the snow pack had high concentrations of DON and NH4+(6.5 and 2.6 µM, respectively), while NO3− concentrations were similar to the snow pack (1.1 µM). The absence of NH4+ in the snowmelt traversing the snow-ice interface may also point to microbial activity occurring at this boundary layer. Melt modelling indicated the presence of liquid meltwater at the snow-ice interface and that at least 10 cm of the surface ice below the snow pack was at 0°C. Solar radiation transmitted through the thin snow pack to the ice surface is absorbed by pigmented glacier algae causing melt of the surrounding ice, allowing the possibly of photosynthesis to begin under the thinning snowpack in these micro-melt environments. Hence, we conjecture that glacier algal blooms can commence before the snow pack has completely melted, occuring at a time when meltwaters are enhanced in nutrients scavenged from the snowpack, superimposed ice and the remnants of the weathering crust from the previous year.
Geometry and Segmentation of the Philippine Fault in Surigao Strait
Authors: Marfito Bryan J. , Llamas Deo Carlo E. , Aurelio Mario A.
Published: March 2022

On 10 February 2017, a MS 6.7 earthquake, whose epicenter was located in Surigao Strait, struck the province of Surigao del Norte, causing numerous geologic impacts and damages to infrastructure. Field investigation conducted immediately after the earthquake revealed an NNW-SSE-striking surface rupture that extends 4.1 km inland. The remaining 20 km of the surface rupture, based on the computed magnitude-surface rupture length empirical relationship, was inferred to be located offshore along the Philippine Fault in Surigao Strait. We investigated the geometry of the fault in Surigao Strait by identifying morphotectonic features and geologic structures using offshore geophysical data that include multibeam bathymetry, sub-bottom profiles, and seismic reflection profiles. We interpreted several fault-related features such as pull-apart basin, offset channels, linear valleys, and folds and identified four fault segments, which are named the Maka-andog Strand, Surigao-Sanghid Strand, West Sanghid Strand, and Panaon Strand. Based on fault length, these segments are capable of generating MW 6.4 to 6.9 earthquakes. We have determined that surface rupture most probably extends along the entire length of the Surigao-Saghid Strand that generated the 2017 MS 6.7 earthquake. The identified morphotectonic features, presence of strike-slip with significant normal displacement in offshore geophysical imaging data, and focal mechanism all suggest that Philippine Fault in Surigao Strait is dominated by transtensional deformation, which supports the interpretations by previous authors.
Research on the Occurrence State of Methane Molecules in Postmature Marine shales—A Case Analysis of the Lower Silurian Longmaxi Formation Shales of the Upper Yangtze Region in Southern China
Authors: Zhang Kun , Song Yan , Jiang Zhenxue , Yuan Xuejiao , Wang Xueying , Han Fengli , Zhang Liwen , Tang Liangyi , Liu Pei , Yang Yiming , Zeng Yao , Chen Xuecheng , Zheng Zehao
Published: March 2022

The Yangtze region in southern China is endowed with abundant marine shale gas. Methane molecules exist in either adsorption state or free state in postmature marine shales, depending on the components of shales. In this study, the core samples of the selected well in the Lower Silurian Longmaxi Fm. shales from the Sichuan Basin, upper Yangtze region, southern China, were taken as study objects. We carried out TOC content, organic matter maturity, mineral component, and core gas content analyses and isothermal adsorption, FIB-SEM, and FIB-HIM experiments to analyze the occurrence state of methane molecules in postmature marine shales. The conclusions are as follows: most methane molecules exist in the organic matter pores of the postmature marine shales, and only a small amount of them exist in clay mineral pores. The organic matter pores in organic-rich shales are large in number with excellent roundness and are well connected, with large pores covering small ones. Thus, abundant free gas can be stored in the organic matter pores and pore throats, making it possible to densely and continuously adsorb methane molecules with a relatively large adsorption space. The flake-shaped clay minerals have a small number of pores with low roundness. Among the three clay minerals in postmature marine shales of the Longmaxi Fm., the I/S mixed layer offers certain reservoir spaces for adsorbed and free gases and chlorite stores a little adsorbed gas and little free gas, while illite hardly stores the adsorbed gas but contains a little free gas.
Wearable System to Guide Crosswalk Navigation for People With Visual Impairment
Authors: Son Hojun , Weiland James
Published: March 2022

Independent travelling is a significant challenge for visually impaired people in urban settings. Traditional and widely used aids such as guide dogs and long canes provide basic guidance and obstacle avoidance but are not sufficient for complex situations such as street crossing. We propose a new wearable system that can safely guide a user with visual impairment at a signalized crosswalk. Safe street crossing is an important element of fully independent travelling for people who are blind or visually impaired (BVI), but street crossing is challenging for BVI because it involves several steps reliant on vision, including scene understanding, localization, object detection, path planning, and path following. Street crossing also requires timely completion. Prior solutions for guiding BVI in crosswalks have focused on either detection of crosswalks or classifying crosswalks signs. In this paper, we demonstrate a system that performs all the functions necessary to safely guide BVI at a signalized crosswalk. Our system utilizes prior maps, similar to how autonomous vehicles are guided. The hardware components are lightweight such that they can be wearable and mobile, and all are commercially available. The system operates in real-time. Computer vision algorithms (Orbslam2) localize the user in the map and orient them to the crosswalk. The state of the crosswalk signal (don’t walk or walk) is detected (using a convolutional neural network), the user is notified (via verbal instructions) when it is safe to cross, and the user is guided (via verbal instructions) along a path towards a destination on the prior map. The system continually updates user position relative to the path and corrects the user’s trajectory with simple verbal commands. We demonstrate the system functionality in three BVI participants. With brief training, all three were able to use the system to successfully navigate a crosswalk in a safe manner.
A Novel Osteochondrodysplasia With Empty Sella Associates With a TBX2 Variant
Authors: Mäkitie Riikka E. , Toiviainen-Salo Sanna , Kaitila Ilkka , Mäkitie Outi
Published: March 2022

Skeletal dysplasias comprise a heterogenous group of developmental disorders of skeletal and cartilaginous tissues. Several different forms have been described and the full spectrum of their clinical manifestations and underlying genetic causes are still incompletely understood. We report a three-generation Finnish family with an unusual, autosomal dominant form of osteochondrodysplasia and an empty sella. Affected individuals (age range 24–44 years) exhibit unusual codfish-shaped vertebrae, severe early-onset and debilitating osteoarthritis and an empty sella without endocrine abnormalities. Clinical characteristics also include mild dysmorphic features, reduced sitting height ratio, and obesity. Whole-exome sequencing excluded known skeletal dysplasias and identified a novel heterozygous missense mutation c.899C>T (p.Thr300Met) in TBX2, confirmed by Sanger sequencing. TBX2 is important for development of the skeleton and the brain and three prior reports have described variations in TBX2 in patients portraying a complex phenotype with vertebral anomalies, craniofacial dysmorphism and endocrine dysfunctions. Our mutation lies near a previously reported disease-causing variant and is predicted pathogenic with deleterious effects on protein function. Our findings expand the current spectrum of skeletal dysplasias, support the association of TBX2 mutations with skeletal dysplasia and suggest a role for TBX2 in development of the spinal and craniofacial structures and the pituitary gland.