bluuebunny/crossref_metadata_2025 huggingface dataset:
1-Isobutanoil-2-isopropylisothiourea Phosphate, T1082: A Safe and Effective Prevention of Radiotherapy Complications in Oncology
Authors: Filimonova Marina , Saburova Alina , Shevchenko Ljudmila , Makarchuk Victoria , Shitova Anna , Soldatova Olga , Rybachuk Vitaly , Kosachenko Alexander , Nikolaev Kirill , Demyashkin Grigory , Saburov Vyacheslav , Koryakin Sergey , Shegay Petr , Kaprin Andrey , Ivanov Sergey , Filimonov Alexander
Published: March 2022

The radioprotective effects of a new 1-isobutanoil-2-isopropylisothiourea derivative named T1082 are presented. Research methods included toxic characteristics, radioprotective activity (Till–McCulloch’s test and 30-day survival test) in γ-ray total-body-irradiated mice, and a clinical and histological study of the effect of T1082 on acute radiation skin reactions (RSR) in rats after a single or fractionated β-ray local irradiation. T1082 is more effective than its analogue, the NOS inhibitor T1023, at low concentrations and doses (1/12–1/8 LD10), both parenterally and intragastrically. In this case, its therapeutic index (LD50/ED50) reaches 30, and the optimal radioprotective doses (ED84–98—141–224 mg/kg) are an order less than the maximum tolerated doses—1/16–1/10 LD10. These properties allowed T1082, at a low intragastrical dose (160 mg/kg; 1/14 LD10), to significantly limit the severity of acute RSR after single (40 Gy) and fractionated (78 Gy) β-ray irradiation. The results confirm T1082 as one of the safest emergency radioprotectors and indicate the prospects for its further development as a pharmacological agent for the prevention of RT complications.
Osteogenic Differentiation of Human Adipose-Derived Stem Cells Seeded on a Biomimetic Spongiosa-like Scaffold: Bone Morphogenetic Protein-2 Delivery by Overexpressing Fascia
Authors: Ren Bin , Betz Oliver B. , Seitz Daniel , Thirion Christian , Salomon Michael , Jansson Volkmar , Müller Peter E. , Betz Volker M.
Published: March 2022

Human adipose-derived stem cells (hADSCs) have the capacity for osteogenic differentiation and, in combination with suitable biomaterials and growth factors, the regeneration of bone defects. In order to differentiate hADSCs into the osteogenic lineage, bone morphogenetic proteins (BMPs) have been proven to be highly effective, especially when expressed locally by route of gene transfer, providing a constant stimulus over an extended period of time. However, the creation of genetically modified hADSCs is laborious and time-consuming, which hinders clinical translation of the approach. Instead, expedited single-surgery gene therapy strategies must be developed. Therefore, in an in vitro experiment, we evaluated a novel growth factor delivery system, comprising adenoviral BMP-2 transduced fascia tissue in terms of BMP-2 release kinetics and osteogenic effects, on hADSCs seeded on an innovative biomimetic spongiosa-like scaffold. As compared to direct BMP-2 transduction of hADSCs or addition of recombinant BMP-2, overexpressing fascia provided a more uniform, constant level of BMP-2 over 30 days. Despite considerably higher BMP-2 peak levels in the comparison groups, delivery by overexpressing fascia led to a strong osteogenic response of hADSCs. The use of BMP-2 transduced fascia in combination with hADSCs may evolve into an expedited single-surgery gene transfer approach to bone repair.
Analytical Assessment of the Vela Diagnostics NGS Assay for HIV Genotyping and Resistance Testing: The Apulian Experience
Authors: Bonifacio Maria Addolorata , Genchi Chiara , Lagioia Antonella , Talamo Vincenza , Volpe Anna , Mariggiò Maria Addolorata
Published: March 2022

Drug-resistance monitoring is one of the hardest challenges in HIV management. Next-generation sequencing (NGS) technologies speed up the detection of drug resistance, allowing the adjustment of antiretroviral therapy and enhancing the quality of life of people living with HIV. Recently, the NGS Sentosa® SQ HIV Genotyping Assay (Vela Diagnostics) received approval for in vitro diagnostics use. This work is the first Italian evaluation of the performance of the Vela Diagnostics NGS platform, assessed with 420 HIV-1 clinical samples. A comparison with Sanger sequencing performance is also reported, highlighting the advantages and disadvantages of the Sentosa® NGS assay. The precision of the technology was studied with reference specimens, while intra- and inter-assay reproducibility were evaluated for selected clinical samples. Vela Diagnostics’ NGS assay reached an 87% success rate through 30 runs of analysis in a real-world clinical context. The concordance with Sanger sequencing outcomes was equal to 97.2%. Several detected mismatches were due to NGS’s superior sensitivity to low-frequency variants. A high accuracy was observed in testing reference samples. Repeatability and reproducibility assays highlighted the good performance of the NGS platform. Beyond a few technical issues that call for further optimization, the key improvement will be a better balance between costs and processing speed. Once these issues have been solved, the Sentosa® SQ HIV Genotyping Assay will be the way forward for HIV resistance testing.
Molecular Cytogenetic Characterization of Novel 1RS.1BL Translocation and Complex Chromosome Translocation Lines with Stripe Rust Resistance
Authors: Li Zhi , Ren Zhenglong , Tan Feiquan , Luo Peigao , Ren Tianheng
Published: March 2022

Rye is the most important source for the genetic improvement of wheat. In this study, two stable wheat-rye primary 1RS.1BL translocation lines, RT855-13 and RT855-14, were selected and identified by acid polyacrylamide gel electrophoresis (A-PAGE), co-dominant PCR, and multi-color fluorescence in situ hybridization (MC-FISH) from the progeny of the crossing of the wheat cultivar Mianyang11 and a Chinese rye Weining. When more than two independent, simple reciprocal translocations are involved in a carrier, they are defined as complex chromosome translocations (CCT). The MC-FISH results also indicated that CCT occurred in RT855-13; namely that, besides 1RS.1BL translocation chromosomes, there are other two pairs of balanced reciprocal translocations. It was demonstrated that the interchange between a distal segment of 4B and long arm of 3D occurred in the RT855-13. The novel translocation chromosomes in wheat were recorded as 3DS.4BSDS and 3DL-4BSPS.4BL. Reports about CCT as a genetic resource in plant breeding programs are scarce. Both lines expressed high resistance to Puccinia striiformis f. sp. tritici, which are prevalent in China and are virulent on Yr9, and the CCT line RT855-13 retained better resistance as adult plants compared with RT855-14 in the field. Both lines, especially the CCT line RT855-13, exhibited better agronomic traits than their wheat parent, Mianyang11, indicating that both translocation lines could potentially be used for wheat improvement. The results also indicated that the position effects of CCT can lead to beneficial variations in agronomic and resistant traits, making them a valuable genetic resource to wheat breeding programs.
Accuracy of Surface Electromyography in the Diagnosis of Pain-Related Temporomandibular Disorders in Children with Awake Bruxism
Authors: Szyszka-Sommerfeld Liliana , Sycińska-Dziarnowska Magdalena , Budzyńska Agata , Woźniak Krzysztof
Published: March 2022

The study assessed masticatory muscle electromyographic (EMG) activity in both children diagnosed with pain-related temporomandibular disorders (TMD-P) and awake bruxism (AB) and in children without TMD, as well as the diagnostic value of surface electromyography (sEMG) in diagnosing TMD-P in subjects with AB. After evaluation based on the Axis I of the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD), 30 children diagnosed with myofascial pain were included in the myofascial pain group and 30 children without TMD diagnosis comprised the control group (mean age of 9.49 ± 1.34 years). The activity of the anterior temporal (TA) and masseter (MM) muscle was assessed bilaterally using a DAB-Bluetooth device (zebris Medical GmBH, Germany) at rest and during maximum voluntary clenching (MVC). The receiver operating characteristic (ROC) curve was used to determine the accuracy, sensitivity, and specificity of the normalized sEMG data. Statistically significant intergroup differences were observed in TA and MM muscle EMG activity at rest and during MVC. Moderate degree of sEMG accuracy in discriminating between TMD-P and non-TMD children was observed for TAmean, left MM, and MMmean EMG muscle activity at rest. sEMG can be a useful tool in assessing myofascial TMD pain in patients with AB.
Development and Validation of a Scoring System for Assessment of Clinical Failure after Pediatric Robot-Assisted Laparoscopic Extravesical Ureteral Reimplantation: A Multi-Center Study
Authors: Koh Chester J. , Kim Kun Suk , Gerber Jonathan A. , Bhatia Vinaya , Zhu Huirong , Baek Minki , Song Sang Hoon
Published: March 2022

We aimed to develop and validate a scoring system as an objective assessment tool for predicting clinical failure after pediatric robotic extravesical ureteral reimplantation. Data for this multi-institutional retrospective cohort was obtained from two tertiary referral hospitals. We defined clinical failure as incomplete radiographic resolution or post-operative febrile UTI. Patients were stratified into low, intermediate, and high-risk groups according to the score. External validation was performed using the model projected to the external validation cohort. An amount of 115 renal units in the development cohort and 46 renal units in the validation cohort were analyzed. The prediction score was calculated with weighted points to each variable according to their regression coefficient as age (year) + BMI + BBD times 10 + VUR grade times 7 + console time (h) + hospital stay times 6. The C-index of our scoring system was 0.850 and 0.770 in the development and validation cohorts, respectively. Clinical failure was significantly different among risk groups: 0% (low-risk), 3.3% (intermediate-risk), and 22.2% (high-risk) (p = 0.004) in the development cohort. A novel scoring system using multiple pre- and intra-operative variables provides a prediction of children at risk of failure after robotic extravesical ureteral reimplantation.
Combined Coronary CT-Angiography and TAVI Planning: Utility of CT-FFR in Patients with Morphologically Ruled-Out Obstructive Coronary Artery Disease
Authors: Gohmann Robin Fabian , Seitz Patrick , Pawelka Konrad , Majunke Nicolas , Schug Adrian , Heiser Linda , Renatus Katharina , Desch Steffen , Lauten Philipp , Holzhey David , Noack Thilo , Wilde Johannes , Kiefer Philipp , Krieghoff Christian , Lücke Christian , Ebel Sebastian , Gottschling Sebastian , Borger Michael A. , Thiele Holger , Panknin Christoph , Abdel-Wahab Mohamed , Horn Matthias , Gutberlet Matthias
Published: March 2022

Background: Coronary artery disease (CAD) is a frequent comorbidity in patients undergoing transcatheter aortic valve implantation (TAVI). If significant CAD can be excluded on coronary CT-angiography (cCTA), invasive coronary angiography (ICA) may be avoided. However, a high plaque burden may make the exclusion of CAD challenging, particularly for less experienced readers. The objective was to analyze the ability of machine learning (ML)-based CT-derived fractional flow reserve (CT-FFR) to correctly categorize cCTA studies without obstructive CAD acquired during pre-TAVI evaluation and to correlate recategorization to image quality and coronary artery calcium score (CAC). Methods: In total, 116 patients without significant stenosis (≥50% diameter) on cCTA as part of pre-TAVI CT were included. Patients were examined with an electrocardiogram-gated CT scan of the heart and high-pitch scan of the torso. Patients were re-evaluated with ML-based CT-FFR (threshold = 0.80). The standard of reference was ICA. Image quality was assessed quantitatively and qualitatively. Results: ML-based CT-FFR was successfully performed in 94.0% (109/116) of patients, including 436 vessels. With CT-FFR, 76/109 patients and 126/436 vessels were falsely categorized as having significant CAD. With CT-FFR 2/2 patients but no vessels initially falsely classified by cCTA were correctly recategorized as having significant CAD. Reclassification occurred predominantly in distal segments. Virtually no correlation was found between image quality or CAC. Conclusions: Unselectively applied, CT-FFR may vastly increase the number of false positive ratings of CAD compared to morphological scoring. Recategorization was virtually independently from image quality or CAC and occurred predominantly in distal segments. It is unclear whether or not the reduced CT-FFR represent true pressure ratios and potentially signifies pathophysiology in patients with severe aortic stenosis.
Risk of Acute Myocardial Infarction in Patients with Gastroenteritis: A Nationwide Case-Control Study
Authors: Tai Ying-Hsuan , Chang Ming-Long , Chu Pao-Hsien , Yeh Chun-Chieh , Cherng Yih-Giun , Chen Ta-Liang , Liao Chien-Chang
Published: March 2022

Gastroenteritis promotes the development of systemic inflammation and a hypercoagulable state. There are limited data regarding the association between gastroenteritis and acute myocardial infarction (AMI). We aimed to evaluate the risk of AMI after an episode of gastroenteritis. In this nested case-control study, we selected patients who were hospitalized for AMI (N = 103,584) as a case group during 2010–2017 and performed propensity score matching (case-control ratio 1:1) to select eligible controls from insurance research data in Taiwan. We applied multivariable logistic regressions to calculate adjusted odds ratios (ORs) with 95% confidence intervals (CIs) for the risk of AMI associated with recent gastroenteritis within 14 days before AMI. We also compared the outcomes after AMI in patients with or without gastroenteritis. A total of 1381 patients (1.3%) with AMI had a prior episode of gastroenteritis compared to 829 (0.8%) among the controls. Gastroenteritis was significantly associated with a subsequent risk of AMI (adjusted OR: 1.68, 95% CI: 1.54–1.83), which was augmented in hospitalizations for gastroenteritis (adjusted OR: 2.50, 95% CI: 1.20–5.21). The outcomes after AMI were worse in patients with gastroenteritis than in those without gastroenteritis, including increased 30-day in-hospital mortality (adjusted OR: 1.28, 95% CI: 1.08–1.52), medical expenditure, and length of hospital stay. Gastroenteritis may act as a trigger for AMI and correlates with worse post-AMI outcomes. Strategies of aggressive hydration and/or increased antithrombotic therapies for this susceptible population should be further developed.
Application of Keratograph and Fourier-Domain Optical Coherence Tomography in Measurements of Tear Meniscus Height
Authors: Chen Minjie , Wei Anji , Xu Jianjiang , Zhou Xingtao , Hong Jiaxu
Published: March 2022

To compare the interoperator repeatability of tear meniscus height (TMH) measurements obtained with a keratograph and Fourier-domain optical coherence tomography (FD-OCT) and to assess the agreement between the methods.Forty-seven eyes with DED and 41 healthy eyes were analyzed using the Schirmer test I and tear breakup time test (TBUT). The TMH was measured three times with each device. The repeatability of measurements was assessed by within-subject standard deviation (Sw), repeatability (2.77 Sw), coefficient of variation (CoV) and intraclass correlation coefficient (ICC). Efficacy in detecting DED was evaluated in terms of the area under the curve (AUC). The TMHs obtained with the keratograph were 0.03 mm lower than those obtained with FD-OCT in both groups (p < 0.001 for the DED group and p = 0.0143 for the control group, respectively). The intraexaminerICCs of the keratographic TMH were 0.789 and 0.817 for the DED and control groups, respectively, and those of the FD-OCT TMH were 0.859 and 0.845, respectively. Although a close correlation was found between the TMHs measured with the keratograph and FD-OCT by the Spearman analysis in both groups (both p < 0.001), poor agreement between the devices was shown in both groups using a Bland–Altman plot. The AUCs of the keratography and FD-OCT results were 0.971 (p < 0.001) and 0.923 (p < 0.001), respectively. Both devices had excellent diagnostic accuracy in differentiating normal patients from DED patients. FD-OCT TMH measurements were more reliable than the keratograph data in the DED group. Agreement between the devices was poor in both groups.
Individual Patterns and Temporal Trajectories of Changes in Fear and Pain during Exposure In Vivo: A Multiple Single-Case Experimental Design in Patients with Chronic Pain
Authors: Bontinck Jente , den Hollander Marlies , Kaas Amanda L. , De Jong Jeroen R. , Timmers Inge
Published: March 2022

Exposure in vivo (EXP) is an effective treatment to reduce pain-related fear and disability in chronic pain populations. Yet, it remains unclear how reductions in fear and pain relate to each other. This single-case experimental design study attempted to identify patterns in the individual responses to EXP and to unravel temporal trajectories of fear and pain. Daily diaries were completed before, during and after EXP. Multilevel modelling analyses were performed to evaluate the overall effect. Temporal effects were scrutinized by individual regression analyses and determination of the time to reach a minimal clinically important difference. Furthermore, individual graphs were visually inspected for potential patterns. Twenty patients with chronic low back pain and complex regional pain syndrome type I were included. On a group level, both fear and pain were reduced following EXP. Individually, fear was significantly reduced in 65% of the patients, while pain in only 20%. A decrease in fear was seen mostly in the first weeks, while pain levels reduced later or remained unchanged. Daily measurements provided rich data on temporal trajectories of reductions in fear and pain. Overall, reductions in fear preceded pain relief and seemed to be essential to achieve pain reductions.
Shaky Student Growth? A Comparison of Robust Bayesian Learning Progress Estimation Methods
Authors: Forthmann Boris , Förster Natalie , Souvignier Elmar
Published: March 2022

Monitoring the progress of student learning is an important part of teachers’ data-based decision making. One such tool that can equip teachers with information about students’ learning progress throughout the school year and thus facilitate monitoring and instructional decision making is learning progress assessments. In practical contexts and research, estimating learning progress has relied on approaches that seek to estimate progress either for each student separately or within overarching model frameworks, such as latent growth modeling. Two recently emerging lines of research for separately estimating student growth have examined robust estimation (to account for outliers) and Bayesian approaches (as opposed to commonly used frequentist methods). The aim of this work was to combine these approaches (i.e., robust Bayesian estimation) and extend these lines of research to the framework of linear latent growth models. In a sample of N = 4970 second-grade students who worked on the quop-L2 test battery (to assess reading comprehension) at eight measurement points, we compared three Bayesian linear latent growth models: (a) a Gaussian model, (b) a model based on Student’s t-distribution (i.e., a robust model), and (c) an asymmetric Laplace model (i.e., Bayesian quantile regression and an alternative robust model). Based on leave-one-out cross-validation and posterior predictive model checking, we found that both robust models outperformed the Gaussian model, and both robust models performed comparably well. While the Student’s t model performed statistically slightly better (yet not substantially so), the asymmetric Laplace model yielded somewhat more realistic posterior predictive samples and a higher degree of measurement precision (i.e., for those estimates that were either associated with the lowest or highest degree of measurement precision). The findings are discussed for the context of learning progress assessment.
Ecological Response of the Subsidy and Incentive System for Grassland Conservation in China
Authors: Lin Huilong , Zhao Yuting , Kalhoro Ghulam Mujtaba
Published: March 2022

The overexploitation of Grasslands without any return-back and compensation is the major cause of degradation and deterioration of the grassland ecosystem. The Subsidy and Incentive System for Grassland Conservation (SISGC) in China aimed to restore grassland ecology by the reduction of overgrazing, promoting carrying capacity, and increasing alternative employment of herders in non-husbandry sectors. However, the ecological response to the SISGC still remains unclear on the national scale. Here, we used systematic sampling, and satellite image time series data revealed a widespread proliferation of major ecological indicators for grasslands, contrasting climate and actual net primary productivity (NPP) before (2004–2010) and after (2011–2017) the implementation of SISGC founded the contributions to policy, as simulated by the Carnegie-Ames-Stanford-Approach (CASA) model. On average, by two-phase comparison, the actual grassland NPP increased by 11.72%. The contribution of policy implementation and climate factors increased grassland NPP by up to 61.14% and 38.86%, respectively, but the response of the NPP growth of various grassland types exhibited divergence, mainly divided into policy-led (contribution rate of 52.28–97.02%) and climate-led (contribution rate of 57.56–96.00%). Hence, the SISGC policies should be renewed for different grassland types.
Gustatory and Saliva Secretory Dysfunctions in COVID-19 Patients with Zinc Deficiency
Authors: Tsuchiya Hironori
Published: March 2022

Given the ever-progressing studies on coronavirus disease 2019 (COVID-19), it is critical to update our knowledge about COVID-19 symptomatology and pathophysiology. In the present narrative review, oral symptoms were overviewed using the latest data and their pathogenesis was hypothetically speculated. PubMed, LitCovid, ProQuest, and Google Scholar were searched for relevant studies from 1 April 2021 with a cutoff date of 31 January 2022. The literature search indicated that gustatory dysfunction and saliva secretory dysfunction are prevalent in COVID-19 patients and both dysfunctions persist after recovery from the disease, suggesting the pathogenic mechanism common to these cooccurring symptoms. COVID-19 patients are characterized by hypozincemia, in which zinc is possibly redistributed from blood to the liver at the expense of zinc in other tissues. If COVID-19 induces intracellular zinc deficiency, the activity of zinc-metalloenzyme carbonic anhydrase localized in taste buds and salivary glands may be influenced to adversely affect gustatory and saliva secretory functions. Zinc-binding metallothioneins and zinc transporters, which cooperatively control cellular zinc homeostasis, are expressed in oral tissues participating in taste and saliva secretion. Their expression dysregulation associated with COVID-19-induced zinc deficiency may have some effect on oral functions. Zinc supplementation is expected to improve oral symptoms in COVID-19 patients.
Bone Marrow Mesenchymal Stem Cells and Their Derived Extracellular Vesicles Attenuate Non-Alcoholic Steatohepatitis-Induced Cardiotoxicity via Modulating Cardiac Mechanisms
Authors: El-Derany Marwa O. , AbdelHamid Sherihan G.
Published: March 2022

Cardiovascular-disease (CVD)-related mortality has been fueled by the upsurge of non-alcoholic steatohepatitis (NASH). Mesenchymal stem cells (MSCs) were extensively studied for their reparative power in ameliorating different CVDs via direct and paracrine effects. Several reports pointed to the importance of bone marrow mesenchymal stem cells (BM-MSCs) as a reliable therapeutic approach for several CVDs. Nevertheless, their therapeutic potential has not yet been investigated in the cardiotoxic state that is induced by NASH. Thus, this study sought to investigate the molecular mechanisms associated with cardiotoxicity that accompany NASH. Besides, we aimed to comparatively study the therapeutic effects of bone-marrow mesenchymal-stem-cell-derived extracellular vesicles (BM-MSCs-EV) and BM-MSCs in a cardiotoxic model that is induced by NASH in rats. Rats were fed with high-fat diet (HFD) for 12 weeks. At the seventh week, BM-MSCs-EV were given a dose of 120 µg/kg i.v., twice a week for six weeks (12 doses per 6 weeks). Another group was treated with BM-MSCs at a dose of 1 × 106 cell i.v., per rat once every 2 weeks for 6 weeks (3 doses per 6 weeks). BM-MSCs-EV demonstrated superior cardioprotective effects through decreasing serum cardiotoxic markers, cardiac hypoxic state (HIF-1) and cardiac inflammation (NF-κB p65, TNF-α, IL-6). This was accompanied by increased vascular endothelial growth factor (VEGF) and improved cardiac histopathological alterations. Both BM-MSCs-EV and BM-MSCs restored the mitochondrial antioxidant state through the upregulation of UCP2 and MnSOD genes. Besides, mitochondrial Parkin-dependent and -independent mitophagies were regained through the upregulation of (Parkin, PINK1, ULK1, BNIP3L, FUNDC1) and (LC3B). These effects were mediated through the regulation of pAKT, PI3K, Hypoxia, VEGF and NF-κB signaling pathways by an array of secreted microRNAs (miRNAs). Our findings unravel the potential ameliorative effects of BM-MSCs-EV as a comparable new avenue for BM-MSCs for modulating cardiotoxicity that is induced by NASH.
Structural and Molecular Characterization of Squalene Synthase Belonging to the Marine Thraustochytrid Species Aurantiochytrium limacinum Using Bioinformatics Approach
Authors: Vyas Sachin , Bettiga Maurizio , Rova Ulrika , Christakopoulos Paul , Matsakas Leonidas , Patel Alok
Published: March 2022

The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the tri-terpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites.
Analysis of the Flatness Form Error in Binder Jetting Process as Affected by the Inclination Angle
Authors: Zago Marco , Lecis Nora , Mariani Marco , Cristofolini Ilaria
Published: March 2022

Binder jetting (BJ) has demonstrated high competitiveness among additive manufacturing processes on account of its high production rate at a low material cost. However, both the design procedure and the process for BJ have to be further developed, aiming towards the proper control of the geometrical and dimensional precision and accuracy of the final product. This paper aims to study the factors that affect the flatness form error. Five geometries were designed to obtain planes that were inclined with respect to the fabrication direction. These planes were measured by a coordinate measuring machine in both the green and sintered state, deriving the best-fitting plane and the flatness form error. The analysis of the green samples demonstrates the prevailing influence of saturation level and layer shifting on flatness form error. In the sintered parts, a dimensional change in sintering can determine shape distortion, or an increment in the surface irregularity observed in the green state. The experimental results clearly evidence the effect of both printing and sintering on the quality of the final product, which should be considered when designing parts to be produced using BJ technology.
Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm
Authors: Feito Javier , Contente Diogo , Ponce-Alonso Manuel , Díaz-Formoso Lara , Araújo Carlos , Peña Nuria , Borrero Juan , Gómez-Sala Beatriz , del Campo Rosa , Muñoz-Atienza Estefanía , Hernández Pablo E. , Cintas Luis M.
Published: March 2022

Probiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.
Genetic Diversity and Relationships of Listeria monocytogenes Serogroup IIa Isolated in Poland
Authors: Lachtara Beata , Wieczorek Kinga , Osek Jacek
Published: March 2022

In the present study, 100 L. monocytogenes isolates of serogroup IIa from food and food production environments in Poland were characterized towards the presence of virulence, resistance, and stress response genes using whole-genome sequencing (WGS). The strains were also molecularly typed and compared with multi-locus sequence typing (MLST) and core genome MLST analyses. The present isolates were grouped into 6 sublineages (SLs), with the most prevalent SL155 (33 isolates), SL121 (32 isolates), and SL8 (28 isolates) and classified into six clonal complexes, with the most prevalent CC155 (33 strains), CC121 (32 isolates), and CC8 (28 strains). Furthermore, the strains were grouped to eight sequence types, with the most prevalent ST155 (33 strains), ST121 (30 isolates), and ST8 (28; strains) followed by 60 cgMLST types (CTs). WGS data showed the presence of several virulence genes or putative molecular markers playing a role in pathogenesis of listeriosis and involved in survival of L. monocytogenes in adverse environmental conditions. Some of the present strains were molecularly closely related to L. monocytogenes previously isolated in Poland. The results of the study showed that food and food production environments may be a source of L. monocytogenes of serogroup IIa with pathogenic potential.
Avian Pathogens: Editorial and the Perspectives of Research
Authors: Sharshov Kirill
Published: March 2022

In the last ten years, humanity has faced new challenges in the field of human and animal health, including emerging viral infections [...]
Publisher’s Note: We Changed Page Numbers to Article Numbers for Articles Published in Nursing Reports Volumes 1–9
Authors:   Nursing Reports Editorial Office
Published: March 2022

Nursing Reports [1] was published by PAGEPress from Volume 1 (2011) to Volume 9 (2019) [...]
Establishing a Resource to Assess Musculoskeletal Health in Older Adults in the Post-COVID-19 Era: Time to SaLSA?
Authors: Laskou Faidra , Linfield Alexander , Aggarwal Pritti , Dennison Elaine M. , Patel Harnish P.
Published: March 2022

Sarcopenia and osteoporosis are associated with morbidity and mortality. The development and progression of these two interrelated conditions are related to genetic and lifestyle factors, including nutrition and physical activity. Restrictions placed on individuals due to the COVID-19 pandemic and infection have led to widespread lifestyle modifications, with data suggesting a negative impact on physical activity levels. There is an urgent need to understand the effect of the pandemic on musculoskeletal health in older adults, at a time when COVID-19 infection and restrictions remain a barrier to research studies. We tested the feasibility of recruiting local community-dwelling older people to establish a new cohort investigating musculoskeletal health—the Southampton Longitudinal Study of Ageing (SaLSA). We invited 1993 community-dwelling older adults registered at the Living Well GP partnership in Southampton, UK, to participate in a study. Questionnaires were completed by participants on health, lifestyle, medication use, comorbidities, physical activity, nutrition, sarcopenia, osteoporosis, and quality of life. Permission was sought for future contact. Descriptive statistics were used on the initial pilot of 175 returned questionnaire data. The median age of participants was 80.4 years in both sexes, 81.3 years (77.9–84) in females, and 81.1 years in males (77.3–83.6). The majority (N = 168/171, 98%) of participants were of white Caucasian background; 36/53 (68%) female participants and 38/119 (32%) male participants lived alone. Over 80% (295/353) consented to be contacted for future studies. Recruitment of participants from a primary care practice into a research study was feasible. The next steps are to perform detailed musculoskeletal phenotyping through physical performance measures, grip strength dynamometry, DXA scanning, high-resolution peripheral quantitative computed tomography (HRpQCT), thigh ultrasound, and muscle biopsy, in a subset of participants. Our vision for SaLSA is to build a platform for discovery science and mechanistic studies, with the goal of improving the health care of older people.
Morphological and Molecular Characterization of a New Myxozoan, Myxobolus grassi sp. nov. (Myxosporea), Infecting the Grass Carp, Ctenopharyngodon idella in the Gomti River, India
Authors: Fariya Naireen , Kaur Harpreet , Singh Mahender , Abidi Rehana , El-Matbouli Mansour , Kumar Gokhlesh
Published: March 2022

Myxosporeans are well-known parasites infecting food fishes in fresh and marine water around the globe. Grass carp (Ctenopharyngodon idella), a freshwater food fish commonly cultured in India with has significant economic importance. Herein, the study focuses on the description of a new myxosporean species, Myxobolus grassi sp. nov. from the gills as primary site and liver as secondary site of infection in grass carp. Both organs (gill and liver) were infected concurrently in the host and the prevalence of grass carp infection was 4.05% in gill filaments and liver, respectively. Identification of species was based on the morphological and morphometric features of the myxospore as well as 18S rDNA sequence data. A smear from gill and liver exhibited hundreds of morphologically similar myxospores. BLAST search revealed 98% sequence similarity and 0.03 genetic distance with M. catlae (KM029967) infecting gill lamellae of mrigal carp (Cirrhinus cirrhosus) from India and 98–84% sequence similarity with other myxobolids in India, China, Japan, Malaysia, Turkey and Hungary. Phylogenetically, it clustered with other myxobolids infecting gills and related organs (i.e., vital organ) of Indian cyprinid carp species. On the basis of myxospore morphology and 18S sequence, we propose M. grassi sp. nov.
Experimental Infection and In-Contact Transmission of H9N2 Avian Influenza Virus in Crows
Authors: Verma Asha Kumari , Kumar Manoj , Murugkar Harshad V. , Nagarajan Shanmugasundaram , Tosh Chakradhar , Namdeo Pushpendra , Singh Rupal , Mishra Suman , Kombiah Subbiah , Dhanapal Senthilkumar , Singh Vijendra Pal
Published: March 2022

This study aimed to investigate the potential of H9N2 avian influenza virus to cause disease and intra-species transmission in house crows (Corvus splendens). A group of six crows were intranasally inoculated with 106.0 EID50 of H9N2 virus (A/chicken/India/07OR17/2021), and 24 h post-inoculation six naïve crows were co-housed with infected crows. Crows were observed for 14 days for any overt signs of illness. Oropharyngeal and cloacal swabs were collected up to 14 days to assess virus excretion. No apparent clinical signs were observed in either infected or in-contact crows. Virus excretion was observed only in infected birds up to 9 days post-infection (dpi) through both oropharyngeal and cloacal routes. All six infected crows seroconverted to H9N2 virus at 14 dpi, whereas all in-contact crows remained negative to H9N2 virus antibodies. No virus could be isolated from tissues viz., lung, liver, kidney, pancreas, small intestine and large intestine. Although crows became infected with the H9N2 virus, transmission of the virus was inefficient to the in-contact group. However, virus excretion through oral and cloacal swabs from infected crows suggests a potential threat for inter-species transmission, including humans. Crows, being a common synanthrope species, might have some role in influenza virus transmission to poultry and humans, which needs to be explored further.
Major Achievements in the Design of Quadruplex-Interactive Small Molecules
Authors: Mendes Eduarda , Aljnadi Israa M. , Bahls Bárbara , Victor Bruno L. , Paulo Alexandra
Published: March 2022

Organic small molecules that can recognize and bind to G-quadruplex and i-Motif nucleic acids have great potential as selective drugs or as tools in drug target discovery programs, or even in the development of nanodevices for medical diagnosis. Hundreds of quadruplex-interactive small molecules have been reported, and the challenges in their design vary with the intended application. Herein, we survey the major achievements on the therapeutic potential of such quadruplex ligands, their mode of binding, effects upon interaction with quadruplexes, and consider the opportunities and challenges for their exploitation in drug discovery.
MdMADS6 Recruits Histone Deacetylase MdHDA19 to Repress the Expression of the Carotenoid Synthesis-Related Gene MdCCD1 during Fruit Ripening
Authors: Li Qiqi , Wang Ting , Xu Chen , Li Meishuo , Tian Ji , Wang Yi , Zhang Xinzhong , Xu Xuefeng , Han Zhenhai , Wu Ting
Published: March 2022

Fruit ripening is regulated by epigenetic modifications and transcription factors, which may function independently or as protein complexes. Our previous study showed that the apple histone deacetylase19 (MdHDA19) suppresses fruit ripening through the deacetylation of histones in related genes. Here, a MADS-box transcription factor (MdMADS6) was identified using a yeast two-hybrid (Y2H) assay as a candidate protein that interacts with MdHDA19 during apple fruit ripening. Furthermore, Y2H, bimolecular fluorescence complementation (BiFC) and pull-down assays were used to confirm the interaction between MdHDA19 and MdMADS6. Agrobacterium-mediated transient transformation and yeast one-hybrid assays showed that MdMADS6 promoted carotenoid accumulation in apple fruit by acting on the downstream target genes related to carotenoid biosynthesis. In summary, we conclude that, in the early stages of fruit development, the expression of MdMADS6 was maintained at lower levels, where it interacted with MdHDA19 to form a protein complex that inhibited the expression of the downstream genes. At the late stages of fruit development, active expression of MdMADS6 dissociated the protein complex of MdMADS6 and MdHDA19 and consequently promoted the expression of carotenoid biosynthesis genes as well as carotenoid accumulation.
Potential Use of Microbial Fuel Cell Technology in Wastewater Treatment
Authors: Koleva Ralitza , Peeva Gergana , Yemendzhiev Hyuseyin , Nenov Valentin
Published: March 2022

Two options, in regard to applying microbial fuel cells (MFCs) in water treatment, are under discussion, namely the conversion of the chemical energy of organic substrates to electricity, as well as the use their potential to reduce different species, such as the ionic form of copper (Cu2+ converted to metal copper) and iron (Fe3+ converted to Fe2+). The high reduction potential of Cu2+ and Fe3+ makes the processes of electricity production and metal reduction, to be performed simultaneously in MFC, achievable. The electrical yield measurement during the experiments of anodic organic matter degradation by MFC in treating an artificial wastewater with chemical oxygen demand (COD) 0.6 and 1.6 g O2·dm−3, as initial COD, are given. It is demonstrated that the higher organic load is associated with better electrical yield. A comparison of MFC and conventional anaerobic digestion performance is discussed, as well. Experimental proofs of copper removal and phosphate mobilization, following the iron reduction of FePO4, are also reported.
Study of the Factors Limiting the Efficiency of Vertical-Type Nitride- and AlInGaP-Based Quantum-Well Micro-LEDs
Authors: Ho Cheng-Han , Chen Shih-Min , Wu Yuh-Renn
Published: March 2022

The efficiency of micro-light-emitting diodes (μ-LEDs) depends enormously on the chip size, and this is connected to sidewall-trap-assisted nonradiative recombination. It is known that the internal quantum efficiency (IQE) of aluminum gallium indium phosphide (AlGaInP)-based red μ-LEDs is much lower than that of nitride-based μ-LEDs. To establish the major reasons giving rise to this huge IQE discrepancy, we examined the limiting factors in the two structures. For the nitride-based InGaN quantum wells, the influences of random alloy fluctuations were examined. A two-dimensional Poisson and drift-diffusion solver was applied to analyze these issues.
Coastal Bathymetry Estimation from Sentinel-2 Satellite Imagery: Comparing Deep Learning and Physics-Based Approaches
Authors: Najar Mahmoud Al , Benshila Rachid , Bennioui Youssra El , Thoumyre Grégoire , Almar Rafael , Bergsma Erwin W. J. , Delvit Jean-Marc , Wilson Dennis G.
Published: March 2022

The ability to monitor the evolution of the coastal zone over time is an important factor in coastal knowledge, development, planning, risk mitigation, and overall coastal zone management. While traditional bathymetry surveys using echo-sounding techniques are expensive and time consuming, remote sensing tools have recently emerged as reliable and inexpensive data sources that can be used to estimate bathymetry using depth inversion models. Deep learning is a growing field of artificial intelligence that allows for the automatic construction of models from data and has been successfully used for various Earth observation and model inversion applications. In this work, we make use of publicly available Sentinel-2 satellite imagery and multiple bathymetry surveys to train a deep learning-based bathymetry estimation model. We explore for the first time two complementary approaches, based on color information but also wave kinematics, as inputs to the deep learning model. This offers the possibility to derive bathymetry not only in clear waters as previously done with deep learning models but also at common turbid coastal zones. We show competitive results with a state-of-the-art physical inversion method for satellite-derived bathymetry, Satellite to Shores (S2Shores), demonstrating a promising direction for worldwide applicability of deep learning models to inverse bathymetry from satellite imagery and a novel use of deep learning models in Earth observation.
Characterization System for Heat-Energy to Electric-Energy Conversion from Concrete by Means of a Thermoelectric Module
Authors: Félix-Herrán Luis C. , García-Juárez Alejandro , García-Delgado Luis Arturo , González-Aguayo Pablo Said , Lozoya-Santos Jorge de-J. , Noriega José R.
Published: March 2022

The present work describes the implementation of a prototype to characterize thermoelectric modules (TEM). The goal is to study the energy conversion by means of thermoelectric modules mounted on concrete structures. The proposed experimental system is used for the electrical characterization of a commercially available thermoelectric module TEC1-12710 to prove its operation while embedded in a concrete slab, typical of building constructions. In this case, the parameters that define thermal energy conversion into electrical energy are open-circuit voltage generation, loaded circuit voltage generation, and load current. A known external load is connected to the terminals of the TEM for the purpose of its electric characterization. An electrical heating element on the hot side and a thermoelectric cooler on the cold side produce a temperature difference on the concrete slab. This arrangement allows the emulation of a temperature gradient produced by sunlight over a concrete structure. The objective is to measure the resulting electrical energy produced by the combination of concrete slab and the thermoelectric module. By controlling the temperature difference between the sides of the thermoelectric module under test, it is possible to simulate the effect of the temperature gradient under different sunlight conditions. Two digital PI controllers regulate the temperature conditions, thus providing controlled conditions for the experiments.
Motion Blur Kernel Rendering Using an Inertial Sensor: Interpreting the Mechanism of a Thermal Detector
Authors: Lee Kangil , Ban Yuseok , Kim Changick
Published: March 2022

Various types of motion blur are frequently observed in the images captured by sensors based on thermal and photon detectors. The difference in mechanisms between thermal and photon detectors directly results in different patterns of motion blur. Motivated by this observation, we propose a novel method to synthesize blurry images from sharp images by analyzing the mechanisms of the thermal detector. Further, we propose a novel blur kernel rendering method, which combines our proposed motion blur model with the inertial sensor in the thermal image domain. The accuracy of the blur kernel rendering method is evaluated by the task of thermal image deblurring. We construct a synthetic blurry image dataset based on acquired thermal images using an infrared camera for evaluation. This dataset is the first blurry thermal image dataset with ground-truth images in the thermal image domain. Qualitative and quantitative experiments are extensively carried out on our dataset, which show that our proposed method outperforms state-of-the-art methods.
Frequency Selective Surface for Ultra-Wide Band Filtering and Shielding
Authors: De Sabata Aldo , Matekovits Ladislau , Buta Adrian , Dassano Gianluca , Silaghi Andrei
Published: March 2022

A frequency selective surface for spatial filtering in the standardized Ultra-Wide Band (UWB) frequency range is proposed. A very large stop-band of 1.75–15.44 GHz has been obtained, with good polarization insensitivity and an angular stability of more than 60∘ and more than 50∘ in TE and TM incidence, respectively. Circuit models have been devised. The structure has been assessed by electromagnetic simulation and implemented on an FR4 substrate of 1.6 mm thickness, with an edge of the square-shaped unit cell of 15 mm. Tests in an anechoic chamber demonstrated good matching between simulation and experimental results and proper operation of the device.