bluuebunny/crossref_metadata_2025 huggingface dataset:
Households’ Willingness to Accept Forest Conservation and Ecosystem Services
Authors: Zhang Meiyan
Published: September 2022

In this study, forest owners’ willingness to accept the governmental redemption of commercial forests for forest conservation, as well as the factors influencing their willingness, was analyzed. It was found that having expected non-timber income from conservation programs, trustable government policies, simpler dealing with government departments for disputes, and satisfactory local ecological condition had strong impacts on the likelihood of participation for the households. If the sum of direct cash compensation incentives and indirect non-timber income compensation incentives was greater than the opportunity costs incurred by forest owners for protection, forest owners were more willing to participate in the redemption. Based on the results, the final offer arbitration method was recommended to improve the maximum price method for redemption, which enables forest owners to receive recognized incentives for direct cash compensation. Ecotourism was strongly recommended to raise forest owners’ expectations of sustainable non-timber income and deliver on such expectations with lower information costs.
Use of Data Augmentation Techniques in Detection of Antisocial Behavior Using Deep Learning Methods
Authors: Maslej-Krešňáková Viera , Sarnovský Martin , Jacková Júlia
Published: September 2022

The work presented in this paper focuses on the use of data augmentation techniques applied in the domain of the detection of antisocial behavior. Data augmentation is a frequently used approach to overcome issues related to the lack of data or problems related to imbalanced classes. Such techniques are used to generate artificial data samples used to improve the volume of the training set or to balance the target distribution. In the antisocial behavior detection domain, we frequently face both issues, the lack of quality labeled data as well as class imbalance. As the majority of the data in this domain is textual, we must consider augmentation methods suitable for NLP tasks. Easy data augmentation (EDA) represents a group of such methods utilizing simple text transformations to create the new, artificial samples. Our main motivation is to explore EDA techniques’ usability on the selected tasks from the antisocial behavior detection domain. We focus on the class imbalance problem and apply EDA techniques to two problems: fake news and toxic comments classification. In both cases, we train the convolutional neural networks classifier and compare its performance on the original and EDA-extended datasets. EDA techniques prove to be very task-dependent, with certain limitations resulting from the data they are applied on. The model’s performance on the extended toxic comments dataset did improve only marginally, gaining only 0.01 improvement in the F1 metric when applying only a subset of EDA methods. EDA techniques in this case were not suitable enough to handle texts written in more informal language. On the other hand, on the fake news dataset, the performance was improved more significantly, boosting the F1 score by 0.1. Improvement was most significant in the prediction of the minor class, where F1 improved from 0.67 to 0.86.
A Fairness Index Based on Rate Variance for Downlink Non-Orthogonal Multiple Access System
Authors: Yang Jie , Zhu Jiajia , Pan Ziyu
Published: September 2022

Aiming at the resource allocation problem of a non-orthogonal multiple access (NOMA) system, a fairness index based on sample variance of users’ transmission rates is proposed, which has a fixed range and high sensitivity. Based on the proposed fairness index, the fairness-constrained power allocation problem in NOMA system is studied; the problem is decoupled into the intra cluster power allocation problem and the inter cluster power allocation problem. The nonconvex optimization problem is solved by the continuous convex approximation (SCA) method, and an intra and inter cluster power iterative allocation algorithm with fairness constrained is proposed to maximize the total throughput. Simulation results show that the proposed algorithm can take into account intra cluster, inter cluster, and system fairness, and maximize the system throughput on the premise of fairness.
Effects of Macleaya cordata Extract on Growth Performance, Serum Biochemical Parameters, and Intestinal Health of Juvenile American Eel (Anguilla rostrata)
Authors: Chen Runan , Huang Linxin , Zhai Shaowei
Published: September 2022

The present trial was conducted to evaluate the supplementation effects of Macleaya cordata extract (MCE) on growth performance, serum biochemical parameters, and intestinal health of the juvenile American eel (Anguilla rostrata). The 480 juvenile American eels (10.93 ± 0.06 g) were randomly divided into four groups. They were fed on diets supplemented with MCE levels of 0, 25 mg/kg, 50 mg/kg, and 100 mg/kg for ten weeks, respectively. The 50 mg/kg or 100 mg/kg MCE could significantly improve growth performance, and increase the activities of acid phosphatase and alkaline phosphatase, as well as the level of high-density lipoprotein cholesterol. These levels of MCE also decreased the levels of D-lactate acid, triglyceride, and total cholesterol and the activities of aspartate aminotransferase, alanine aminotransferase, and diamine oxidase. The antioxidant ability, muscular thickness, and fold height of the intestine were enhanced by 50 mg/kg or 100 mg/kg MCE. There was no significant difference in the above parameters of groups fed with 50 mg/kg or 100 mg/kg of MCE. The beneficial effects on the intestinal microbiota were demonstrated in the group fed with 50 mg/kg MCE. In conclusion, the 50 mg/kg MCE could be used in the diet to improve the growth performance and health status of the juvenile American eels.
Effect of Pomegranate Extract Consumption on Satiety Parameters in Healthy Volunteers: A Preliminary Randomized Study
Authors: Stockton Angela , Al-Dujaili Emad A. S.
Published: September 2022

There has been an increasing interest in nutraceuticals and functional foods in reducing appetite and to lose weight. We assessed the effect of oral pomegranate extract (PE) and PE juice (PJ) intake vs. placebo on satiety parameters in healthy volunteers. Twenty-eight subjects (mean age 34.5 ± 13.7 years, body mass index [BMI] 25.05 ± 3.91 kg/m2) were randomized to 3-week priming supplementation with PE (Pomanox®) or placebo. On week 3, satiety parameters were determined on 1 testing day after participants ingested a breakfast and a lunch meal with PJ juice, using 100-mm visual acuity scales (VAS) for hunger, desire to eat, fullness and satisfaction. Meal quality and palatability were also tested. The desire to eat was less at all time points in the PJ juice with PE priming group and participants were also less hungry (p = 0.044) than those who consumed placebo. There was an overall significant difference between the groups (p < 0.001). Participants in the PJ juice with PE priming group experienced significantly greater satisfaction (p = 0.036) and feeling of fullness (p = 0.02) than those in the placebo group. These findings suggest that consumption of PE could have the potential to modulate satiety indicators.
The Influence of Trial-By-Trial Feedback on Trust in Health, First-Episode and Chronic Psychosis
Authors: Lemmers-Jansen Imke L. J. , Wichmann Rune J. , Perizonius Sophie , Shergill Sukhi S.
Published: September 2022

Trust is crucial to establishing reciprocal, positive social interactions and seems to be compromised in psychosis. The trust game offers methods to assess an individual’s trust responses to trust-reciprocating, positive feedback. Various computational techniques have been implemented to measure trust responsiveness, mostly based on investments. Here, we propose a new method, focusing on feedback response. Psychosis patients show social dysfunction and reduced trust during early and more progressed illness stages. The present study inspects differences in feedback responsiveness of 102 first-episode psychosis patients (FEPs), 43 chronic psychosis patients (CPs), and 39 healthy controls (HCs) by adopting a novel assessment approach. Additionally, baseline trust, the trust exerted without any prior knowledge of the partner’s trustworthiness, and mean trust were examined. Participants performed a multi-round trust game, playing the investor role, and were paired with a computer, programmed to return at least the invested amount, representing a trustworthy partner. The new method detected group differences, more distinguished than the former methods. Contrary to our expectations, baseline trust was intact in patients. Relative to HCs, patients were less responsive to feedback, failing to integrate the positive information into their decision-making process. The magnitude of returns was not associated with increases in trust. This novel method showed promising results and confirmed patients’ deficits within the social interactional domain.
Hydroxyapatite Reinforced Polyvinyl Alcohol/Polyvinyl Pyrrolidone Based Hydrogel for Cartilage Replacement
Authors: Jalageri Mallikarjun B. , Mohan Kumar G. C.
Published: September 2022

Polyvinyl alcohol (PVA) and Polyvinyl Pyrrolidone (PVP) hydrogels are desirable biomaterials for soft tissue repair and replacement. However, the bio-inertness and poor cell adhesive potency of the PVA and PVP hinder the wide range of biomedical applications. In the present work, PVA and PVP were blended with a one-dimensional hydroxyapatite nanorod (HNr), and PVA/PVP/HNr composite hydrogel was synthesized by the freeze-thaw process. The developed hydrogels were characterized by Scanning Electron Microscope (SEM). The bio-ceramic nanohydroxyapatite content was optimized, and it was found that reinforcement improves mechanical strength as well as bioactivity. The compression strength values are 2.47 ± 0.73 MPa for the composite having 2 wt% of nanohydroxyapatite. The storage modulus was much higher than the loss modulus, which signifies the elastic dominancy similar to cartilage. Besides, the antimicrobial activity of nanohydroxyapatite reinforced PVA hydrogel towards bacterial species, Escherichia coli (E. Coli), Staphylococcus aureus (S. aureus) was satisfactory, and the in vitro biocompatibility response towards Human Mesenchymal stem cells(hMSC) after 72 h of culture confirms nanohydroxyapatite reinforced PVA/PVP hydrogels are the promising alternatives for next-generation cartilage substitutes.
Some Remarkable Rheological and Conducting Properties of Hybrid PVC Thermoreversible Gels/Organogels
Authors: Collin Dominique , Viswanatha-Pillai Ganesh , Vargas Jentzsch Andreas , Gavat Odile , Moulin Emilie , Giuseppone Nicolas , Guenet Jean-Michel
Published: September 2022

We report on investigations into the rheological properties of organogels prepared from triarylamine trisamide (TATA) and oligo phenylene vinylene (OPVOH) molecules in binary organogel gels and in ternary thermoreversible networks with poly vinyl chloride (PVC). In the case of OPVOH, we show that the modulus of the ternary gel is simply the sum of the modulus of each binary gel, corresponding to the so-called Voigt upper limit. In contrast, TATA/PVC ternary gels generally exceed the Voigt upper limit. In an attempt to account for this unexpected outcome, we hypothesized that a de-solvation process might occur in the PVC fibrils that possibly originates in the propensity of TATA molecules to form molecular compounds with the solvent. Finally, the conducting properties of TATA/solvent organogels and TAT/PVC/solvent reversible networks were measured. It was found that they strongly depend on the solvent type but are not significantly altered when PVC is present. Therefore, PVC gels can be made conducive by incorporating TATA fibers.
Transcriptomic Analysis Reveals the Correlation between End-of-Day Far Red Light and Chilling Stress in Setaria viridis
Authors: Sun Shilei , Liu Qingjia , Dai Xiuru , Wang Xianglan
Published: September 2022

Low temperature and end-of-day far-red (EOD-FR) light signaling are two key factors limiting plant production and geographical location worldwide. However, the transcriptional dynamics of EOD-FR light conditions during chilling stress remain poorly understood. Here, we performed a comparative RNA-Seq-based approach to identify differentially expressed genes (DEGs) related to EOD-FR and chilling stress in Setaria viridis. A total of 7911, 324, and 13431 DEGs that responded to low temperature, EOD-FR and these two stresses were detected, respectively. Further DEGs analysis revealed that EOD-FR may enhance cold tolerance in plants by regulating the expression of genes related to cold tolerance. The result of weighted gene coexpression network analysis (WGCNA) using 13431 nonredundant DEGs exhibited 15 different gene network modules. Interestingly, a CO-like transcription factor named BBX2 was highly expressed under EOD-FR or chilling conditions. Furthermore, we could detect more expression levels when EOD-FR and chilling stress co-existed. Our dataset provides a valuable resource for the regulatory network involved in EOD-FR signaling and chilling tolerance in C4 plants.
Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases
Authors: Hilt Evann E. , Ferrieri Patricia
Published: September 2022

Next-generation sequencing (NGS) technologies have become increasingly available for use in the clinical microbiology diagnostic environment. There are three main applications of these technologies in the clinical microbiology laboratory: whole genome sequencing (WGS), targeted metagenomics sequencing and shotgun metagenomics sequencing. These applications are being utilized for initial identification of pathogenic organisms, the detection of antimicrobial resistance mechanisms and for epidemiologic tracking of organisms within and outside hospital systems. In this review, we analyze these three applications and provide a comprehensive summary of how these applications are currently being used in public health, basic research, and clinical microbiology laboratory environments. In the public health arena, WGS is being used to identify and epidemiologically track food borne outbreaks and disease surveillance. In clinical hospital systems, WGS is used to identify multi-drug-resistant nosocomial infections and track the transmission of these organisms. In addition, we examine how metagenomics sequencing approaches (targeted and shotgun) are being used to circumvent the traditional and biased microbiology culture methods to identify potential pathogens directly from specimens. We also expand on the important factors to consider when implementing these technologies, and what is possible for these technologies in infectious disease diagnosis in the next 5 years.
First Genome of Rock Lizard Darevskia valentini Involved in Formation of Several Parthenogenetic Species
Authors: Ochkalova Sofia , Korchagin Vitaly , Vergun Andrey , Urin Avel , Zilov Danil , Ryakhovsky Sergei , Girnyk Anastasiya , Martirosyan Irena , Zhernakova Daria V. , Arakelyan Marine , Danielyan Felix , Kliver Sergei , Brukhin Vladimir , Komissarov Aleksey , Ryskov Alexey
Published: September 2022

The extant reptiles are one of the most diverse clades among terrestrial vertebrates and one of a few groups with instances of parthenogenesis. Due to the hybrid origin of parthenogenetic species, reference genomes of the parental species as well as of the parthenogenetic progeny are indispensable to explore the genetic foundations of parthenogenetic reproduction. Here, we report on the first genome assembly of rock lizard Darevskia valentini, a paternal species for several parthenogenetic lineages. The novel genome was used in the reconstruction of the comprehensive phylogeny of Squamata inferred independently from 7369 trees of single-copy orthologs and a supermatrix of 378 conserved proteins. We also investigated Hox clusters, the loci that are often regarded as playing an important role in the speciation of animal groups with drastically diverse morphology. We demonstrated that Hox clusters of D. valentini are invaded with transposons and contain the HoxC1 gene that has been considered to be lost in the amniote ancestor. This study provides confirmation for previous works and releases new genomic data that will contribute to future discoveries on the mechanisms of parthenogenesis as well as support comparative studies among reptiles.
Hearing Loss in Stickler Syndrome: An Update
Authors: Acke Frederic R. E. , De Leenheer Els M. R.
Published: September 2022

Stickler syndrome is a connective tissue disorder characterized by ocular, skeletal, orofacial and auditory manifestations. Its main symptoms are high myopia, retinal detachment, joint hypermobility, early osteoarthritis, cleft palate, midfacial hypoplasia, micrognathia and hearing loss. Large phenotypical variability is apparent and partly explained by the underlying genetic heterogeneity, including collagen genes (COL2A1, COL11A1, COL11A2, COL9A1, COL9A2, COL9A3) and non-collagen genes (BMP4, LRP2, LOXL3). The most frequent type of Stickler syndrome (COL2A1) is characterized by a rather mild high-frequency sensorineural hearing loss in about half of the patients. COL11A1- and COL11A2-related Stickler syndrome results in more frequent hearing loss, being moderate and involving all frequencies. Hearing loss in the rarer types of Stickler syndrome depends on the gene expression in the cochlea, with moderate to severe downsloping hearing loss for Stickler syndrome caused by biallelic type IX collagen gene mutations and none or mild hearing loss for the non-collagen genes. Inherent to the orofacial manifestations, middle ear problems and temporary conductive hearing loss, especially at young age, are also prevalent. Consequently, hearing loss should be actively sought for and adequately treated in Stickler syndrome patients given its high prevalence and the concomitant visual impairment in most patients.
Prenatal Diagnosis of Talipes Equinovarus by Ultrasound and Chromosomal Microarray Analysis: A Chinese Single-Center Retrospective Study
Authors: Huang Ruibin , Yang Xin , Zhou Hang , Fu Fang , Cheng Ken , Wang You , Ma Chunling , Li Ru , Jing Xiangyi , Han Jin , Zhen Li , Pan Min , Li Dongzhi , Liao Can
Published: September 2022

Background: There are few studies on the detection rate by chromosomal microarray analysis (CMA) of the prenatal diagnosis of talipes equinovarus (TE) compared to conventional karyotyping. We aimed to explore the molecular etiology of fetal TE and examine the detection rate by CMA, which provides more information for the clinical screening and genetic counseling of TE. Methods: In this retrospective study, pregnancies diagnosed with fetal TE were enrolled and clinical data for all cases were retrieved from our medical record database, including demographic data for pregnancies, ultrasound findings, karyotype/CMA results, and pregnant and perinatal outcomes. Results: Among the 164 patients, 17 (10.4%) clinically significant variants were detected by CMA. In 148 singleton pregnancies, the diagnostic rate of clinically significant variants was significantly higher in the non-isolated TE group than in the isolated TE group (10/37, 27.0% vs. 6/111, 5.4%, P < 0.001). In twin pregnancies, 1 (6.3%) pathogenic copy number variant was present in the other 16 twin pregnancies. Conclusions: This study demonstrates that CMA is useful for the prenatal genetic diagnosis of fetal TE. Fetal TE with the associated structural malformation correlates with a higher probability of clinically significant variants. This data may aid prenatal diagnosis and genetic counseling for fetal TE.
Exploratory Analysis of Outpatient Visits for US Adults Diagnosed with Lupus Erythematosus: Findings from the National Ambulatory Medical Care Survey 2006–2016
Authors: Preciado Salena Marie , Elsaid Khaled A. , Fawaz Souhiela , Brown Lawrence , Seoane-Vazquez Enrique , Fleming Marc , Wang Yun
Published: September 2022

The study aims to assess office-based visit trends for lupus patients and evaluate their medication burden, chronic conditions, and comorbidities. This cross-sectional study used data from the National Ambulatory Medical Care Survey (NAMCS), a survey sample weighted to represent national estimates of outpatient visits. Adult patients diagnosed with lupus were included. Medications and comorbidities that were frequently recorded were identified and categorized. Descriptive statistics and bivariate analyses were used to characterize visits by sex, age, race/ethnicity, insurance type, region, and reason for visit. Comorbidities were identified using diagnosis codes documented at each encounter. There were 27,029,228 visits for lupus patients from 2006 to 2016, and 87% them were on or were prescribed medications. Most visits were for female (88%), white (79%), non-Hispanic (88%) patients with private insurance (53%). The majority of patients were seen for a chronic routine problem (75%), and 29% had lupus as the primary diagnosis. Frequent medications prescribed were hydroxychloroquine (30%), prednisone (23%), multivitamins (14%), and furosemide (9%). Common comorbidities observed included arthritis (88%), hypertension (25%), and depression (13%). Prescription patterns are reflective of comorbidities associated with lupus. By assessing medications most frequently prescribed and comorbid conditions among lupus patients, we showcase the complexity of disease management and the need for strategies to improve care.
Quality of Life in Post-COVID-19 Patients after Hospitalization
Authors: Mitrović-Ajtić Olivera , Stanisavljević Dejana , Miljatović Sanja , Dragojević Teodora , Živković Emilija , Šabanović Miloš , Čokić Vladan P.
Published: September 2022

The COVID-19 pandemic has had a strong impact on people’s quality of life (QoL), which is affected by social and economic changes as well as by mental and physical health. The aim of this study was to determine QoL in post-COVID-19 patients who had required hospitalization, and to identify relevant sociodemographic data. We used questionnaires which considered demographic and socioeconomic data, health and vaccination status, the pandemic situation, and EQ-5D scoring. The interactions of all data and the scores of EQ-5D were analyzed. Multivariate logistic regression analysis was applied to the five dimensions of EQ-5D. In this single-hospital-cohort study, the average times elapsed since initial diagnosis and hospital admission were 2.5 (76.3 ± 18.1 days) and 5 months (155.4 ± 33.9 days), respectively. Post-COVID-19 females were 3–5 times more likely to be affected in terms of anxiety/depression, and in negative impact upon their usual activities, at 5 months after diagnosis. At the same time, reductions in mobility were 3–4 times more likely in elderly post-COVID-19 patients, whose levels of pain and discomfort increased. Single patients, those with low incomes, and those with severe clinical outcomes were 2–4 times more likely to experience a reduction in their usual activities, while the presence of co-morbidities and lower levels of education were associated with increased pain and discomfort. Aging-induced pain/discomfort and anxiety/depression were significantly exacerbated in elderly patients with widespread vaccination. Our study revealed effects of demographic and socioeconomic factors upon lower QoL in post-COVID-19 patients in four dimensions of EQ-5D: mobility, usual activity, pain/discomfort, and anxiety/depression, 5 months after first diagnosis and hospitalization.
Basics of Sustainable Diets and Tools for Assessing Dietary Sustainability: A Primer for Researchers and Policy Actors
Authors: Alexandropoulou Ioanna , Goulis Dimitrios G. , Merou Theodora , Vassilakou Tonia , Bogdanos Dimitrios P. , Grammatikopoulou Maria G.
Published: September 2022

Climate change can have economic consequences, affecting the nutritional intake of populations and increasing food insecurity, as it negatively affects diet quality parameters. One way to mitigate these consequences is to change the way we produce and consume our food. A healthy and sustainable diet aims to promote and achieve the physical, mental, and social well-being of the populations at all life stages, while protecting and safeguarding the resources of the planet and preserving biodiversity. Over the past few years, several indexes have been developed to evaluate dietary sustainability, most of them based on the EAT-Lancet reference diet. The present review explains the problems that arise in human nutrition as a result of climate change and presents currently available diet sustainability indexes and their applications and limitations, in an effort to aid researchers and policy actors in identifying aspects that need improvement in the development of relevant indexes. Overall, great heterogeneity exists among the indicators included in the available indexes and their methodology. Furthermore, many indexes do not adequately account for the diets’ environmental impact, whereas others fall short in the economic impact domain, or the ethical aspects of sustainability. The present review reveals that the design of one environmentally friendly diet that is appropriate for all cultures, populations, patients, and geographic locations is a difficult task. For this, the development of sustainable and healthy diet recommendations that are region-specific and culturally specific, and simultaneously encompass all aspects of sustainability, is required.
Social Support and Resilience as Predictors of Prosocial Behaviors before and during COVID-19
Authors: Esparza-Reig Javier , Martí-Vilar Manuel , González-Sala Francisco , Merino-Soto César , Toledano-Toledano Filiberto
Published: September 2022

The objective of this research was to analyze the relationship between social support and resilience with prosocial behavior before and during the confinement caused by COVID-19. Materials and Methods: The participants were divided into a confined group (228 women and 84 men) and an unconfined group (153 women and 105 men), all of whom were university students. Instruments were applied to measure the variables proposed. Results: Social support predicted 24.4% of the variance in prosocial behavior among women and 12% among men in the confined group; no evidence of this relationship was found in the unconfined groups. Resilience predicted 7% of the variance in prosocial behavior among confined women, 8.4% among confined men, 8.8% among unconfined women, and 5.1% in unconfined men. Discussion and Conclusion: The results show the importance of social support and resilience in prosocial behaviors, which are key elements for the proper functioning of society, especially in the face of a crisis such as COVID-19.
Regeneration of Cotoneaster wilsonii Nakai through Indirect Organogenesis
Authors: Li Yali , Xiao Jie , Jeong Byoung Ryong
Published: September 2022

The ornamental plant Cotoneaster wilsonii Nakai is a rare endemic species to the Ulleung Island in Korea. There is an urgent need to develop efficient propagation methods to preserve this endangered plant species. The cytokinin thidiazuron (TDZ) at a concentration of 0, 1.0, 2.0, or 3.0 mg·L−1 combined with 0, 0.5, 1.0, or 1.5 mg·L−1 a-naphthaleneacetic acid (NAA) was used to induce calli from young leaf explants on the Murashige and Skoog (MS) medium. The TDZ and/or 6-benzyladenine at a concentration of 0, 0.5, or 1.0 mg·L−1 combined with 0.2 mg·L−1 NAA was supplied to the MS medium to induce shoots from calli. Four auxins at 0, 0.2, 0.4, 0.6, 0.8, or 1.0 mg·L−1 were supplied to half-strength MS medium for root induction. The treatment of TDZ at 1.0 mg·L−1 combined with 1.5 mg·L−1 NAA not only had 100% callus induction ratio, but also induced the heaviest calli. The shoot induction ratio was the greatest with TDZ at 1.0 mg·L−1, and the greatest root induction ratio was obtained with IAA at a concentration of 0.8 mg·L−1. In conclusion, an effective indirect organogenesis system was established for C. wilsonii Nakai.
Comparative Effects of Four Plant Growth Regulators on Yield and Field Performance of Crocus sativus L.
Authors: Heidari Fatemeh , Shekari Farid , Andalibi Babak , Saba Jalal , Uberti Daniela , Mastinu Andrea
Published: September 2022

The effects of four plant growth regulators on Crocus sativus L. (saffron) yield and performance were studied in two consecutive years under field conditions. Saffron corms were immersed in solutions of gibberellic acid (GA3), salicylic acid (SA), paclobutrazol (PBZ), chlormequat chloride (CCC), distilled water (hydroprime), and dry corms as the control. Results showed that among the different treatments, plants primed with GA3 500 μM had the highest flower fresh weight and stigma dry weight. In contrast, primed corms with 1000 μM CCC and 100 μM PBZ had the lowest flower dry weight. Furthermore, the plants primed with SA 1400 had the highest leaf numbers, leaf dry weight, and leaf area index (LAI). Plants treated with GA3 induced narrow but the longest leaves, while those treated with SA showed the widest ones. It was also demonstrated that the application of CCC and PBZ can produce shorter leaves. Furthermore, the greatest numbers of daughter corms were obtained in 1400 μM SA. Both PBZ and CCC were reported to have no impacts on the corm numbers but produced larger and heavier daughter corms. The results revealed that the priming of saffron corms with GA3 and then SA improved saffron growth and yield.
Efficacy and Comparison of Different Strategies for Selenium Biofortification of Tomatoes
Authors: Shiriaev Anton , Pezzarossa Beatrice , Rosellini Irene , Malorgio Fernando , Lampis Silvia , Ippolito Antonio , Tonutti Pietro
Published: September 2022

At appropriate concentrations, selenium (Se) is beneficial for humans. Tomato appears to be one of the best commodities for producing Se-biofortified fruit for dietary supplementation. To assess the efficacy of different enrichment protocols, a total of four on-plant and off-plant trials were conducted. Hydroponically grown tomato plants were sprayed with: (i) chemically synthesized Se nanoparticles (SeNPs) at 0, 1, and 1.5 mg Se L−1 at blooming; (ii) sodium selenate (Na2SeO4) or SeNPs solution at 0, 5, and 10 mg Se L−1 when the fruit entered the immature green stage. With regard to the off-plant trials, harvested mature green fruit were immersed in Na2SeO4 solution: (iii) at 0, 5, 10, and 20 mg Se L−1 for 15 s under a vacuum; (iv) at 0, 40, and 80 mg Se L−1 for 1 h. Spraying Na2SeO4 induced higher Se accumulation in plant tissue than SeNPs: both protocols were effective in enriching tomatoes. Postharvest Se enrichment via vacuum infiltration caused textural damage, whereas passive immersion in solution induced fruit Se accumulation without causing any damage. SeNPs appear to be quantitatively less effective than Na2SeO4, but might be environmentally safer. Elemental Se carried by NPs may be more easily incorporated into organic forms, which are more bioavailable for humans. Passive immersion may represent an alternative Se-enrichment strategy, allowing for the biofortification of harvested tomato fruit directly, with lower risks of environmental pollution.
Considering Imperial Complexity in Prehistory: A Polyethnic Wari Enclave in Moquegua, Peru
Authors: Nash Donna J.
Published: September 2022

Wari is thought by many to be the first Andean Empire (ca. 600–1000 AD); however, the means of expansion, the areas controlled, the strength of the polity, and the nature of Wari institutions remain largely contested. In general, models describing the Wari polity are simplistic and do not exploit sophisticated approaches developed by historical archaeologists. Wari expansion into the Moquegua Valley, Peru, was originally interpreted as an intrusive colony or distant outpost, perhaps to engage its southern neighbor, Tiwanaku. It was presumed that migrants from the polity’s core established settlements and imperial infrastructure in an unoccupied ecozone. Recent research of households in the colony reveals diverse domestic material culture, diets, and use of living space. Those who lived in Wari-affiliated settlements were probably drawn from other imperial provinces and communities from other areas of the drainage. Forced relocations are common among historically documented empires, but willing pioneers may have selected for this tenuous frontier. Regional-scale data show that Wari had the strength to change the local economy and control this distant frontier province; household-scale data reveal the polyethnic nature of the colonial enclave and provide clues to understand early imperial institutions.
Thyroid Diseases and Thyroid Asymptomatic Dysfunction in People Living With HIV
Authors: Micali Cristina , Russotto Ylenia , Celesia Benedetto Maurizio , Santoro Laura , Marino Andrea , Pellicanò Giovanni Francesco , Nunnari Giuseppe , Venanzi Rullo Emmanuele
Published: September 2022

Thyroid diseases (TDs) and thyroid asymptomatic dysfunctions (TADs) are correlated with Human Immunodeficiency virus (HIV) infection and Acquired ImmunoDeficiency Syndrome (AIDS) as well as many endocrine dysfunctions and dysregulation of hormonal axes. To date, available studies on People Living With HIV (PLWH) affected by thyroid diseases and asymptomatic dysfunctions are few and rather controversial. The purpose of the present non-systematic literature review is to recap the current knowledge on the main features of thyroid dysfunctions and disorders in PLWH. Large cohort studies are needed for a better comprehension of the impact, evolution and treatment of thyroid pathologies in the HIV-infected population.
Spatial Distributions and Intrinsic Influence Analysis of Cr, Ni, Cu, Zn, As, Cd and Pb in Sediments from the Wuliangsuhai Wetland, China
Authors: Zhang Huilan , Liang Piaopiao , Liu Ying , Wang Xinglei , Bai Yahong , Xing Yunxin , Wei Chunli , Li Yuanyuan , Liu Yiming , Hu Yu
Published: September 2022

The spatial distributions of Cr, Ni, Cu, Zn, As, Cd and Pb (potentially toxic elements, PTEs) in sediments and intrinsic influence factors from the Wuliangsuhai wetland of the Hetao Irrigation District, China were studied in this work. The results showed that excluding Zn, the total contents of other PTEs were higher than the background values, of which As (39.26 mg·kg−1) and Cd (0.44 mg·kg−1) were six-fold and seven-fold higher, respectively. Especially, the high levels of Cd (70.17%), Pb (66.53%), and Zn (57.20%) in the non-residual fraction showed high bioavailability and mobility. It indicated that PTEs can enter the food chain more easily and produce much toxicity. Based on Igeo, ICF, and MRI, the contamination of As was the most serious in the middle areas (MDP) of the wetland, and its risk was up to moderately strong. Cd and Pb posed moderate and considerate risk, respectively. Furthermore, 29.50% and 55.54% risk contribution ratio of As and Cd, respectively, showed that they were the dominant contaminants. In addition, the positive correlation between sand, OM, and total contents and chemical fractions of PTEs by using PCM, RDA, and DHCA indicated that physicochemical properties could significantly influence the spatial distributions of PTEs. The work was useful for assessing the level of pollution in the study area and acquiring information for future and possible monitoring and remediation activities.
Effects of Exercise on Patients with Obstructive Sleep Apnea: A Systematic Review and Meta-Analysis
Authors: Peng Jiale , Yuan Yuling , Zhao Yuanhui , Ren Hong
Published: September 2022

With exercise being more frequently utilized in treatment for obstructive sleep apnea (OSA), a systematic review of the intervention efficacy of exercise on OSA is necessary. PubMed, EBSCO, Web of Science, VIP, and CNKI databases were searched to collect randomized controlled trials (RCTs) of exercise applied to OSA from January 2000 to January 2022. The literature screening, data extraction, and risk of bias assessment of included studies were conducted independently by two reviewers. Meta-analysis was then performed using Rev Man 5.4 software. A total of 9 RCTs were included, including 444 patients. Compared with the control group, exercise made an improvement in apnea–hypopnea index (AHI) [MD = −6.65, 95% CI (−7.77, −5.53), p < 0.00001], minimum oxygen saturation (SaO2min%) [MD = 1.67, 95% CI (0.82, 2.52), p = 0.0001], peak oxygen uptake (VO2peak) [SMD = 0.54, 95% CI (0.31, 0.78), p < 0.00001], Pittsburgh sleep quality index (PSQI) [MD = −2.08, 95% CI (−3.95, −0.21), p = 0.03], and Epworth Sleepiness Scale (ESS) values [MD = −1.64, 95% CI, (−3.07, −0.22), p = 0.02]. However, there were no significant changes in body mass index (BMI). As for the results of subgroup analysis, aerobic exercise combined with resistance exercise [MD = −7.36, 95% CI (−8.64, −6.08), p < 0.00001] had a better effect on AHI reduction than aerobic exercise alone [MD = −4.36, 95% CI (−6.67, −2.06), p = 0.0002]. This systematic review demonstrates that exercise reduces the severity of OSA with no changes in BMI, and the effect of aerobic exercise combined with resistance training is better than aerobic exercise alone in AHI reduction. Exercise also improves cardiopulmonary fitness, sleep quality, and excessive daytime sleepiness.
Impacts of Transitioning to an Online Curriculum at a Graduate School in South Korea Due to the COVID-19 Pandemic
Authors: Yee Eric , Jung Changhwa , Cheriberi Derrick , Choi Minjune , Park Wonsick
Published: September 2022

This study focuses on the impacts of implementing an online curriculum at a graduate school in South Korea in response to the COVID-19 pandemic. A framework distinguishing impacts to academic, educational, and institutional stakeholders from the virtualization of curricula as well as general COVID-19 prevention measures is invoked to help understand the impacts of these changes. These impacts are sourced from general graduate school operations, course evaluations for two compulsory courses, and unofficial interviews with students and professors. A statistical evaluation of the course evaluations suggested no significant difference between the online format of 2020 and the traditional in person formats in prior years in terms of academics and education. Unofficial meetings with students and faculty revealed technical issues throughout 2020, which many could not be resolved due to the variety of different computer systems at the school as well as limited technical support. Most importantly, students stated they were suffering from prolonged mental and emotional distress such as feeling isolated. Lessons learned include having academic institutions prepare for difficulties in technical support, educational infrastructure investments, compliance, as well as student body mental health.
Does 8-Week Resistance Training with Slow Movement Cadenced by Pilates Breathing Affect Muscle Strength and Balance of Older Adults? An Age-Matched Controlled Trial
Authors: Fernandes Ilha G. , Macedo Maria C. G. S. , Souza Matheus A. , Silveira-Nunes Gabriela , Barbosa Michelle C. S. A. , Queiroz Andreia C. C. , Vieira Edgar R. , Barbosa Alexandre C.
Published: September 2022

This study investigated the balance and dorsiflexion strength of older adults after eight weeks of resistance training, with the exercise velocity cadenced by the Pilates breathing technique and the volume modulated by the session duration. Forty-four older adults were divided into two groups: resistance training (TR; n = 22) and resistance training with the Pilates breathing technique cadencing all exercises (TR + P; n = 22), both during eight weeks. The total exercising volume was controlled by time of execution (50 min/session). The dorsiflexion strength and balance were assessed. The RT group showed higher dorsiflexion strength after the protocol: Right (RT = 29.1 ± 7.7 vs. RT + P = 22.9 ± 5.2, p = 0.001) and Left (RT = 29.5 ± 6.9 vs. RT + P = 24.0 ± 5.2, p = 0.001). All balance parameters were improved in RT + P group compared to its own baseline: Path Length (cm) (pre = 71.0 ± 14.3 vs. post = 59.7 ± 14.3, p = 0.003); Sway Velocity (cm/s) (pre = 3.6 ± 0.7; post = 2.9 ± 0.7; p = 0.001); Sway Area (cm2) (pre = 8.9 ± 5.3 vs. post = 5.7 ± 2.1, p = 0.003); Excursion Medio Lateral (cm) (pre = 3.0 ± 0.7 vs. post = 2.6 ± 0.5 cm, p = 0.002); and Excursion AP (cm) (pre = 3.6 ± 1.4 vs. post = 2.8 ± 0.7 cm, p = 0.010). Resistance training using slower velocity movement cadenced by Pilates breathing technique produced balance improvements compared to baseline (moderate to large effect sizes), but no between-group effect was observed at the end of the protocol. The dorsiflexion strength was higher in the RT group compared to RT + P group.
Associations among Active Commuting to School and Prevalence of Obesity in Adolescents: A Systematic Review
Authors: Martin-Moraleda Evelyn , Mandic Sandy , Queralt Ana , Romero-Blanco Cristina , Aznar Susana
Published: September 2022

Active commuting to school (ACS) seems to be one of the means to increase physical activity (PA) levels in youth, but it is unclear if ACS reduces the prevalence of obesity, protecting and improving their health. Most of the previous research has been conducted on children or youth (i.e., children with adolescents together), and there is a paucity of research in adolescents only. The purpose of this review was to assess the association between ACS with overweight/obesity parameters in adolescents aged 11 to 19 years. We used PubMed, WOS and SPORTDiscus as electronics databases. All steps of the process followed the recommendations of the PRISMA flow-diagram. Fifteen articles (68.18%) found a consistent association between ACS and body composition and seven studies (31.82%) showed no differences in body composition between active and passive commuters to school. Fourteen studies observed that active commuters to school had a more favorable body composition and one study reported that ACS was associated with unfavourable body composition. ACS could be the steppingstone to improve PA promotion in adolescence but whether ACS is associated with improved body composition and prevention of obesity requires further research.
High-Coverage Reconstruction of XCO2 Using Multisource Satellite Remote Sensing Data in Beijing–Tianjin–Hebei Region
Authors: Wang Wei , He Junchen , Feng Huihui , Jin Zhili
Published: September 2022

The extreme climate caused by global warming has had a great impact on the earth’s ecology. As the main greenhouse gas, atmospheric CO2 concentration change and its spatial distribution are among the main uncertain factors in climate change assessment. Remote sensing satellites can obtain changes in CO2 concentration in the global atmosphere. However, some problems (e.g., low time resolution and incomplete coverage) caused by the satellite observation mode and clouds/aerosols still exist. By analyzing sources of atmospheric CO2 and various factors affecting the spatial distribution of CO2, this study used multisource satellite-based data and a random forest model to reconstruct the daily CO2 column concentration (XCO2) with full spatial coverage in the Beijing–Tianjin–Hebei region. Based on a matched data set from 1 January 2015, to 31 December 2019, the performance of the model is demonstrated by the determination coefficient (R2) = 0.96, root mean square error (RMSE) = 1.09 ppm, and mean absolute error (MAE) = 0.56 ppm. Meanwhile, the tenfold cross-validation (10-CV) results based on samples show R2 = 0.91, RMSE = 1.68 ppm, and MAE = 0.88 ppm, and the 10-CV results based on spatial location show R2 = 0.91, RMSE = 1.68 ppm, and MAE = 0.88 ppm. Finally, the spatially seamless mapping of daily XCO2 concentrations from 2015 to 2019 in the Beijing–Tianjin–Hebei region was conducted using the established model. The study of the spatial distribution of XCO2 concentration in the Beijing–Tianjin–Hebei region shows its spatial differentiation and seasonal variation characteristics. Moreover, daily XCO2 map has the potential to monitor regional carbon emissions and evaluate emission reduction.
Early Maladaptive Schemas and Self-Stigma in People with Physical Disabilities: The Role of Self-Compassion and Psychological Flexibility
Authors: Pyszkowska Anna , Stojek Monika M.
Published: September 2022

Self-stigmatizing thoughts may be rooted in one’s core beliefs, and in turn be associated with early maladaptive schemas (EMS). Psychological flexibility, an ability to distance and accept one’s thoughts, is reported to diminish EMS’s effect on well-being, while self-compassion, a mindful attitude towards one’s suffering, often reduces self-stigma. The objective of this study was to examine associations between EMS, self-stigma, psychological flexibility and self-compassion in individuals with disabilities, as they are at higher risk of experiencing self-stigma. Participants were 238 persons with disabilities. The Self-Stigma Scale, Young’s Schemas Questionnaire, the Self-Compassion Scale Short and the Acceptance and Action-II Questionnaire were used. Hierarchical regression and mediation analysis were used to establish (1) predictors and (2) potential mediators of self-stigma in people with disabilities. Hierarchical regression showed that EMS alone accounted for 39% of the variance explained by self-stigma, and with the addition of psychological flexibility—an additional 2% was explained. Parallel mediation analyses indicated that psychological flexibility partially mediated the relationship between EMS domains and self-stigma. It appears that psychological rigidity is related to self-stigma and should be addressed in treatment through evidence-based approaches such as Schema Therapy and Acceptance and Commitment Therapy to enhance individuals’ healthy life patterns, flexibility and self-compassion.
Cannabidiol (CBD) Protects Adipose-Derived Mesenchymal Stem Cells (ASCs) against Endoplasmic Reticulum Stress Development and Its Complications
Authors: Kowalczuk Anna , Marycz Krzysztof , Kornicka-Garbowska Katarzyna , Kornicka Justyna , Bujalska-Zadrożny Magdalena , Groborz Sylwia
Published: September 2022

Background: Recent studies suggested that individuals with metabolic disorders have altered function of adipocytes and adipose stem cell subpopulations, which impairs tissue homeostasis, promoting insulin resistance and diabetes development. The non-psychoactive phytocannabinoid CBD was found to modulate adipose tissue metabolism, however, its exact role in controlling ASCs’ fate is still poorly understood. Objectives: This investigation aimed to elucidate whether pretreatment of ASCs with CBD can protect against ER stress development and maintain the cytophysiological properties of cells. Methods: Human ASCs were cultured under control and adipogenic conditions. Prior to the experiments, cells in the experimental group were pretreated with CBD following the addition of an ER stress inducer—tunicamycin. After the experiments, the cells were subsequently tested for expression of the apoptotic, ER stress, and anti-inflammatory-related genes using RT-qPCR. Oxidative stress was analysed with flow cytometric assays. Results: Cells pretreated with CBD displayed decreased apoptosis and enhanced proliferation rate. Additionally, the expression of pro-inflammatory cytokines and miRNAs was significantly reduced. The obtained results also demonstrated an obvious reduction in intracellular accumulated ROS and NO, as well as mitigated ER stress through the down-regulation of IRE-1, PERK, CHOP, and ATF6 transcripts upon CBD treatment. Conclusion: The presented data provide the evidence that CBD protects ASCs against ER stress development and its complications and, thus, offers new insights for the management of obesity through the regulation of adipose tissue dynamics.