bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating Maternal Stress, Depression, and Breastfeeding: A Pregnancy Risk Assessment Monitoring System (2016–2019) Analysis
Authors: Dhaurali Shubhecchha , Dugat Vickie , Whittler Tayler , Shrestha Shikhar , Kiani Marwah , Ruiz Maria Gabriela , Ali Iman , Enge Courtney , Amutah-Onukagha Ndidiamaka
Published: June 2023

Breastfeeding is invaluable for postpartum physical healing and mental wellbeing, but psychosocial stress and depression impede such recovery processes. To inform future interventions and policies, associations between breastfeeding, maternal stress, and depression were examined. Data from the Pregnancy Risk Assessment Monitoring System (PRAMS) were analyzed (2016–2019). Logistic regression models were used to calculate adjusted odds ratios with 95% confidence intervals. Of the total sample (n = 95,820), approximately 88% of participants attempted breastfeeding. Our findings indicate that participants who experienced any form of stress had a slightly higher likelihood of breastfeeding compared to those without stress. Specifically, partner-related and financial-related stressors were significantly associated with increased odds of breastfeeding. However, no significant associations were observed trauma-related or emotional-related stressors and breastfeeding. Additionally, no significant association was found between depression at different stages (preconception, prenatal, and postpartum) and breastfeeding. A significant interaction effect was noted between having experienced any of the 13 stressors and Black race/ethnicity on breastfeeding odds. Similarly, significant interaction effects were observed between partner-related, trauma, financial, or emotional stressors and Black race/ethnicity. These findings emphasize the importance of considering various factors when promoting breastfeeding in diverse populations, and screening for psychosocial stress during postpartum visits. Our study recommends tailoring breastfeeding interventions to address the needs of Black mothers which could significantly improve maternal health and breastfeeding outcomes.
Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C
Authors: Elazab Doaa , Capuana Maurizio , Ozudogru Elif Aylin , Anichini Monica , Lambardi Maurizio
Published: June 2023

ElecTIS is a new single container bioreactor which does not require forced air blowing, instead making the culture material mobile and the liquid medium stationary. The timed up-and-down movement of the basket containing the shoot culture ensures periodic contact with the liquid medium positioned at the base of the container. In this study we tested for the first time its use in the recovery of blackberry shoot cultures (Rubus fruticosus L., cvs Thornfree and Chester), coming from 5 months of slow growth storage (SGS), at 4 °C and in the dark. The shoot recovery at standard culture conditions was performed on two different types of ElecTIS, i.e., one with a smaller basket (ElecTISS, 234 cm2 of culture area), and one with a large basket (ElecTISL, 336 cm2), comparing the culture in TIS (cycle of 8 min every 6 h, equal to 32 min/day) with the traditional one in a gelled medium in glass jars (500 cc). After each one of the three 4-week subcultures, the shoot growth parameters and the relative growth rate highlighted a clear superiority of ElecTIS in promoting the recovery of shoot cultures coming from SGS. The analyses of chlorophyll content and stoma functionality confirmed the superior quality of shoots cultured in the ElecTIS bioreactor, and these shoots were afterwards easily rooted and acclimatized ex vitro.
NextGEM: Next-Generation Integrated Sensing and Analytical System for Monitoring and Assessing Radiofrequency Electromagnetic Field Exposure and Health
Authors: Petroulakis Nikolaos , Mattsson Mats-Olof , Chatziadam Panos , Simko Myrtill , Gavrielides Andreas , Yiorkas Andrianos M. , Zeni Olga , Scarfi Maria Rosaria , Soudah Eduardo , Otin Ruben , Schettino Fulvio , Migliore Marco Donald , Miaoudakis Andreas , Spanoudakis George , Bolte John , Korkmaz Erdal , Theodorou Vasileios , Zarogianni Eleni , Lagorio Susanna , Biffoni Mauro , Schiavoni Andrea , Boldi Mauro Renato , Feldman Yuri , Bilik Igal , Laromaine Anna , Gich Martí , Spirito Marco , Ledent Maryse , Segers Seppe , Vargas Francisco , Colussi Loek , Pruppers Mathieu , Baaken Dan , Bogdanova Anna
Published: June 2023

The evolution of emerging technologies that use Radio Frequency Electromagnetic Field (RF-EMF) has increased the interest of the scientific community and society regarding the possible adverse effects on human health and the environment. This article provides NextGEM’s vision to assure safety for EU citizens when employing existing and future EMF-based telecommunication technologies. This is accomplished by generating relevant knowledge that ascertains appropriate prevention and control/actuation actions regarding RF-EMF exposure in residential, public, and occupational settings. Fulfilling this vision, NextGEM commits to the need for a healthy living and working environment under safe RF-EMF exposure conditions that can be trusted by people and be in line with the regulations and laws developed by public authorities. NextGEM provides a framework for generating health-relevant scientific knowledge and data on new scenarios of exposure to RF-EMF in multiple frequency bands and developing and validating tools for evidence-based risk assessment. Finally, NextGEM’s Innovation and Knowledge Hub (NIKH) will offer a standardized way for European regulatory authorities and the scientific community to store and assess project outcomes and provide access to findable, accessible, interoperable, and reusable (FAIR) data.
Irisin Protects against Loss of Trabecular Bone Mass and Strength in Adult Ovariectomized Mice by Stimulating Osteoblast Activity
Authors: Storlino Giuseppina , Dicarlo Manuela , Zerlotin Roberta , Pignataro Patrizia , Sanesi Lorenzo , Suriano Clelia , Oranger Angela , Mori Giorgio , Passeri Giovanni , Colucci Silvia , Grano Maria , Colaianni Graziana
Published: June 2023

Irisin is a peptide secreted by skeletal muscle that plays a major role in bone metabolism. Experiments in mouse models have shown that administration of recombinant irisin prevents disuse-induced bone loss. In this study, we aimed to evaluate the effects of irisin treatment for the prevention of bone loss in the ovariectomized (Ovx) mouse, the animal model commonly used to investigate osteoporosis caused by estrogen deficiency. Micro-Ct analysis conducted on Sham mice (Sham-veh) and Ovx mice treated with vehicle (Ovx-veh) or recombinant irisin (Ovx-irisn) showed bone volume fraction (BV/TV) decreases in femurs (Ovx-veh 1.39± 0.71 vs. Sham-veh 2.84 ± 1.23; p = 0.02) and tibia at both proximal condyles (Ovx-veh 1.97 ± 0.68 vs. Sham-veh 3.48 ± 1.26; p = 0.03) and the subchondral plate (Ovx-veh 6.33 ± 0.36 vs. Sham-veh 8.18 ± 0.41; p = 0.01), which were prevented by treatment with a weekly dose of irisin for 4 weeks. Moreover, histological analysis of trabecular bone showed that irisin increased the number of active osteoblasts per bone perimeter (Ovx-irisin 32.3 ± 3.9 vs. Ovx-veh 23.5 ± 3.6; p = 0.01), while decreasing osteoclasts (Ovx-irisin 7.6 ± 2.4 vs. Ovx-veh 12.9 ± 3.04; p = 0.05). The possible mechanism by which irisin enhances osteoblast activity in Ovx mice is upregulation of the transcription factor Atf4, one of the key markers of osteoblast differentiation, and osteoprotegerin, thereby inhibiting osteoclast formation.
Dendritic Cell Subpopulations Are Associated with Morphological Features of Breast Ductal Carcinoma In Situ
Authors: Szpor Joanna , Streb Joanna , Glajcar Anna , Streb-Smoleń Anna , Łazarczyk Agnieszka , Korta Paulina , Brzuszkiewicz Karolina , Jach Robert , Hodorowicz-Zaniewska Diana
Published: June 2023

Ductal carcinoma in situ (DCIS) is the preinvasive form of breast cancer (BC). It is disputed whether all cases of DCIS require extensive treatment as the overall risk of progression to BC is estimated at 40%. Therefore, the crucial objective for researchers is to identify DCIS with significant risk of transformation into BC. Dendritic cells (DC) are professional antigen presenting cells and as such play a pivotal role in the formation of immune cells that infiltrate in breast tumors. The aim of this study was to investigate the relationship between the density of DCs with different superficial antigens (CD1a, CD123, DC-LAMP, DC-SIGN) and various histopathological characteristics of DCIS. Our evaluation indicated that CD123+ and DC-LAMP+ cells were strongly associated with maximal tumor size, grading and neoductgenesis. Together with CD1a+ cells, they were negatively correlated with hormonal receptors expression. Furthermore, the number of DC-LAMP+ cells was higher in DCIS with comedo necrosis, ductal spread, lobular cancerization as well as comedo-type tumors, while CD1a+ cells were abundant in cases with Paget disease. We concluded that different subpopulations of DCs relate to various characteristics of DCIS. Of the superficial DCs markers, DC-LAMP seems particularly promising as a target for further research in this area.
Prohibitin Links Cell Cycle, Motility and Invasion in Prostate Cancer Cells
Authors: Koushyar Sarah , Uysal-Onganer Pinar , Jiang Wen Guo , Dart Dafydd Alwyn
Published: June 2023

Prohibitin (PHB) is a tumour suppressor gene with several different molecular activities. PHB overexpression leads to G1/S-phase cell cycle arrest, and PHB represses the androgen receptor (AR) in prostate cancer cells. PHB interacts with and represses members of the E2F family in a manner that may also be AR-linked, therefore making the AR:PHB:E2F interaction axis highly complex. PHB siRNA increased the growth and metastatic potential of LNCaP mouse xenografts in vivo. Conversely, PHB ectopic cDNA overexpression affected several hundred genes in LNCaP cells. Furthermore, gene ontology analysis showed that in addition to cell cycle regulation, several members of the WNT family were significantly downregulated (WNT7B, WNT9A and WNT10B), as well as pathways for cell adhesion. Online GEO data studies showed PHB expression to be decreased in clinical cases of metastatic prostate cancer, and to be correlated with higher WNT expression in metastasis. PHB overexpression reduced prostate cancer cell migration and motility in wound-healing assays, reduced cell invasion through a Matrigel layer and reduced cellular attachment. In LNCaP cells, WNT7B, WNT9A and WNT10B expression were also upregulated by androgen treatment and downregulated by androgen antagonism, indicating a role for AR in the control of these WNT genes. However, these WNTs were strongly cell cycle regulated. E2F1 cDNA ectopic expression and PHB siRNA (both cell cycle promoting effects) increased WNT7B, WNT9A and WNT10B expression, and these genes were also upregulated as cells were released from G1 to S phase synchronisation, indicating further cell cycle regulation. Therefore, the repressive effects of PHB may inhibit AR, E2F and WNT expression and its loss may increase metastatic potential in human prostate cancer.
Role of Estrogen and Estrogen Receptor in GH-Secreting Adenomas
Authors: Voltan Giacomo , Mazzeo Pierluigi , Regazzo Daniela , Scaroni Carla , Ceccato Filippo
Published: June 2023

Acromegaly is a rare disease with several systemic complications that may lead to increased overall morbidity and mortality. Despite several available treatments, ranging from transsphenoidal resection of GH-producing adenomas to different medical therapies, complete hormonal control is not achieved in some cases. Some decades ago, estrogens were first used to treat acromegaly, resulting in a significant decrease in IGF1 levels. However, due to the consequent side effects of the high dose utilized, this treatment was later abandoned. The evidence that estrogens are able to blunt GH activity also derives from the evidence that women with GH deficiency taking oral estro-progestins pills need higher doses of GH replacement therapy. In recent years, the role of estrogens and Selective Estrogens Receptor Modulators (SERMs) in acromegaly treatment has been re-evaluated, especially considering poor control of the disease under first- and second-line medical treatment. In this review, we analyze the state of the art concerning the impact of estrogen and SERMs on the GH/IGF1 axis, focusing on molecular pathways and the possible implications for acromegaly treatment.
Injection-Molded Isotactic Polypropylene Colored with Green Transparent and Opaque Pigments
Authors: Janostik Vaclav , Senkerik Vojtech , Manas Lukas , Stanek Michal , Cvek Martin
Published: June 2023

Polypropylene (PP) belongs among the most important commodity plastics due to its widespread application. The color of the PP products can be achieved by the addition of pigments, which can dramatically affect its material characteristics. To maintain product consistency (dimensional, mechanical, and optical), knowledge of these implications is of great importance. This study investigates the effect of transparent/opaque green masterbatches (MBs) and their concentration on the physico-mechanical and optical properties of PP produced by injection molding. The results showed that selected pigments had different nucleating abilities, affecting the dimensional stability and crystallinity of the product. The rheological properties of pigmented PP melts were affected as well. Mechanical testing showed that the presence of both pigments increased the tensile strength and Young’s modulus, while the elongation at break was significantly increased only for the opaque MB. The impact toughness of colored PP with both MBs remained similar to that of neat PP. The optical properties were well controlled by the dosing of MBs, and were further related to the RAL color standards, as demonstrated by CIE color space analysis. Finally, the selection of appropriate pigments for PP should be considered, especially in areas where dimensional and color stability, as well as product safety, are highly important.
Role of the Sodium-Dependent Organic Anion Transporter (SOAT/SLC10A6) in Physiology and Pathophysiology
Authors: Wannowius Marie , Karakus Emre , Aktürk Zekeriya , Breuer Janina , Geyer Joachim
Published: June 2023

The sodium-dependent organic anion transporter (SOAT, gene symbol SLC10A6) specifically transports 3′- and 17′-monosulfated steroid hormones, such as estrone sulfate and dehydroepiandrosterone sulfate, into specific target cells. These biologically inactive sulfo-conjugated steroids occur in high concentrations in the blood circulation and serve as precursors for the intracrine formation of active estrogens and androgens that contribute to the overall regulation of steroids in many peripheral tissues. Although SOAT expression has been detected in several hormone-responsive peripheral tissues, its quantitative contribution to steroid sulfate uptake in different organs is still not completely clear. Given this fact, the present review provides a comprehensive overview of the current knowledge about the SOAT by summarizing all experimental findings obtained since its first cloning in 2004 and by processing SOAT/SLC10A6-related data from genome-wide protein and mRNA expression databases. In conclusion, despite a significantly increased understanding of the function and physiological significance of the SOAT over the past 20 years, further studies are needed to finally establish it as a potential drug target for endocrine-based therapy of steroid-responsive diseases such as hormone-dependent breast cancer.
Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis
Authors: Singh Nivisti , Sithole Bishop Bruce , Govinden Roshini
Published: June 2023

Pulp and paper mill sludge (PPMS) is currently disposed of into landfills which are reaching their maximum capacity. Valorisation of PPMS by enzymatic hydrolysis using cellulases is an alternative strategy. Existing commercial cellulases are expensive and contain low titres of β-glucosidases. In this study, β-glucosidase production was optimised by Aspergillus japonicus VIT-SB1 to obtain higher β-glucosidase titres using the One Variable at a Time (OVAT), Plackett Burman (PBD), and Box Behnken design (BBD)of experiments and the efficiency of the optimised cellulase cocktail to hydrolyse cellulose was tested. β-Glucosidase production was enhanced from 0.4 to 10.13 U/mL, representing a 25.3-fold increase in production levels after optimisation. The optimal BBD production conditions were 6 days of fermentation at 20 °C, 125 rpm, 1.75% soy peptone, and 1.25% wheat bran in (pH 6.0) buffer. The optimal pH for β-glucosidase activity in the crude cellulase cocktail was (pH 5.0) at 50 °C. Optimal cellulose hydrolysis using the crude cellulase cocktail occurred at longer incubation times, and higher substrate loads and enzyme doses. Cellulose hydrolysis with the A. japonicus VIT-SB1 cellulase cocktail and commercial cellulase cocktails resulted in glucose yields of 15.12 and 12.33 µmol/mL glucose, respectively. Supplementation of the commercial cellulase cocktail with 0.25 U/mg of β-glucosidase resulted in a 19.8% increase in glucose yield.
Vitamin E Analog Trolox Attenuates MPTP-Induced Parkinson’s Disease in Mice, Mitigating Oxidative Stress, Neuroinflammation, and Motor Impairment
Authors: Atiq Abubakar , Lee Hyeon Jin , Khan Amjad , Kang Min Hwa , Rehman Inayat Ur , Ahmad Riaz , Tahir Muhammad , Ali Jawad , Choe Kyonghwan , Park Jun Sung , Kim Myeong Ok
Published: June 2023

Trolox is a potent antioxidant and a water-soluble analog of vitamin E. It has been used in scientific studies to examine oxidative stress and its impact on biological systems. Trolox has been shown to have a neuroprotective effect against ischemia and IL-1β-mediated neurodegeneration. In this study, we investigated the potential protective mechanisms of Trolox against a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease mouse model. Western blotting, immunofluorescence staining, and ROS/LPO assays were performed to investigate the role of trolox against neuroinflammation, the oxidative stress mediated by MPTP in the Parkinson’s disease (PD) mouse model (wild-type mice (C57BL/6N), eight weeks old, average body weight 25–30 g). Our study showed that MPTP increased the expression of α-synuclein, decreased tyrosine hydroxylase (TH) and dopamine transporter (DAT) levels in the striatum and substantia nigra pars compacta (SNpc), and impaired motor function. However, Trolox treatment significantly reversed these PD-like pathologies. Furthermore, Trolox treatment reduced oxidative stress by increasing the expression of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Lastly, Trolox treatment inhibited the activated astrocytes (GFAP) and microglia (Iba-1), also reducing phosphorylated nuclear factor-κB, (p-NF-κB) and tumor necrosis factor-alpha (TNF-α) in the PD mouse brain. Overall, our study demonstrated that Trolox may exert neuroprotection on dopaminergic neurons against MPTP-induced oxidative stress, neuroinflammation, motor dysfunction, and neurodegeneration.
Electrocatalytic Reactions for Converting CO2 to Value-Added Products: Recent Progress and Emerging Trends
Authors: Masoumi Zohreh , Tayebi Meysam , Tayebi Mahdi , Masoumi Lari S. Ahmad , Sewwandi Nethmi , Seo Bongkuk , Lim Choong-Sun , Kim Hyeon-Gook , Kyung Daeseung
Published: June 2023

Carbon dioxide (CO2) emissions are an important environmental issue that causes greenhouse and climate change effects on the earth. Nowadays, CO2 has various conversion methods to be a potential carbon resource, such as photocatalytic, electrocatalytic, and photo-electrocatalytic. CO2 conversion into value-added products has many advantages, including facile control of the reaction rate by adjusting the applied voltage and minimal environmental pollution. The development of efficient electrocatalysts and improving their viability with appropriate reactor designs is essential for the commercialization of this environmentally friendly method. In addition, microbial electrosynthesis which utilizes an electroactive bio-film electrode as a catalyst can be considered as another option to reduce CO2. This review highlights the methods which can contribute to the increase in efficiency of carbon dioxide reduction (CO2R) processes through electrode structure with the introduction of various electrolytes such as ionic liquid, sulfate, and bicarbonate electrolytes, with the control of pH and with the control of the operating pressure and temperature of the electrolyzer. It also presents the research status, a fundamental understanding of carbon dioxide reduction reaction (CO2RR) mechanisms, the development of electrochemical CO2R technologies, and challenges and opportunities for future research.
New 3-Aminopropylsilatrane Derivatives: Synthesis, Structure, Properties, and Biological Activity
Authors: Adamovich Sergey N. , Ushakov Igor A. , Oborina Elizaveta N. , Lukyanova Svetlana V. , Komarov Vladislav Y.
Published: June 2023

The biologically active compound 3-aminopropylsilatrane (a compound with a pentacoordinated silicon atom) underwent an aza-Michael reaction with various acrylates and other Michael acceptors. Depending on the molar ratio, the reaction yielded Michael mono- or diadducts (11 examples) containing functional groups (silatranyl, carbonyl, nitrile, amino, etc.). These compounds were characterized via IR and NMR spectroscopy, mass spectrometry, X-ray diffraction, and elemental analysis. Calculations (using in silico, PASS, and SwissADMET online software) revealed that the functionalized (hybrid) silatranes were bioavailable, druglike compounds that exhibited pronounced antineoplastic and macrophage-colony-stimulating activity. The in vitro effect of silatranes on the growth of pathogenic bacteria (Listeria, Staphylococcus, and Yersinia) was studied. It was found that the synthesized compounds exerted inhibitory and stimulating effects in high and low concentrations, respectively.
Celiac Disease and the Risk of Cardiovascular Diseases
Authors: Wang Yichen , Chen Bing , Ciaccio Edward J. , Jneid Hani , Virani Salim S. , Lavie Carl J. , Lebovits Jessica , Green Peter H. R. , Krittanawong Chayakrit
Published: June 2023

Celiac disease (CD) is a chronic autoimmune disorder that affects the small intestine in genetically predisposed individuals. Previous studies have investigated the potential link between CD and cardiovascular disease (CVD); however, the findings have been inconsistent. We aimed to provide an updated review of the literature on the association between CD and CVD. PubMed was searched from inception to January 2023 using keywords including CD, cardiovascular disease, coronary artery disease, cardiac arrhythmia, heart failure, cardiomyopathy, and myocarditis. We summarized the results of the studies, including meta-analyses and original investigations, and presented them according to the different forms of CVD. Meta-analyses published in 2015 provided mixed results regarding the relationship between CD and CVD. However, subsequent original investigations have shed new light on this association. Recent studies indicate that individuals with CD are at a higher risk of developing overall CVD, including an increased risk of myocardial infarction and atrial fibrillation. However, the link between CD and stroke is less established. Further research is needed to determine the link between CD and other cardiac arrhythmias, such as ventricular arrhythmia. Moreover, the relationship between CD and cardiomyopathy or heart failure, as well as myopericarditis, remains ambiguous. CD patients have a lower prevalence of traditional cardiac risk factors, such as smoking, hypertension, hyperlipidemia, and obesity. Therefore, it is important to discover strategies to identify patients at risk and reduce the risk of CVD in CD populations. Lastly, it is unclear whether adherence to a gluten-free diet can diminish or increase the risk of CVD among individuals with CD, necessitating further research in this area. To fully comprehend the correlation between CD and CVD and to determine the optimal prevention strategies for CVD in individuals with CD, additional research is necessary.
Basalt-Fiber-Reinforced Phosphorus Building Gypsum Composite Materials (BRPGCs): An Analysis on Their Working Performance and Mechanical Properties
Authors: Wu Lei , Tao Zhong , Huang Ronggui , Zhang Zhiqi , Shen Jinjin , Xu Weijie
Published: June 2023

The preparation of fiber-reinforced phosphorus building gypsum composite materials (FRPGCs) is an important approach to enlarge the utilization of phosphogypsum resources. Through reinforcing phosphorus building gypsum (PBG) with basalt fiber (BF), this article probes into the effects of the length and fiber content of BF on the working performance and mechanical properties of basalt-fiber-reinforced phosphorus building gypsum composite materials (BRPGCs) and accesses the toughness of BRPGCs under bending loads using residual strength. The results showed that the addition of BF could significantly promote the mechanical properties of BRPGCs. However, due to the adverse effect of fibers on the working performance of BRPGCs, the fiber content was constrained. After adding 1.2% of 6 mm BF, the bending strength and compressive strength of FRPGCs reached maximum values of 10.98 MPa and 29.83 MPa, respectively. Under a bending load, BRPGCs exhibited an apparent ductile behavior. The P-δ curve presented five stages, with an evident phase of strength stability after cracking. A larger fiber content was conducive to the toughness of BRPGCs. When 1.6% of 6 mm BF was added, the residual strength of FRPGCs could reach 6.77 MPa.
Molecular Switching through Chalcogen-Bond-Induced Isomerization of Binuclear (Diaminocarbene)PdII Complexes
Authors: Popov Roman A. , Novikov Alexander S. , Suslonov Vitalii V. , Boyarskiy Vadim P.
Published: June 2023

Binuclear diaminocarbene complexes, which form as a regioisomer mixture in the reaction between isocyanide–palladium(II) complex cis-[PdCl2(CNXyl)2] and 1,3-thiazol-2-amine, are able to exchange an anionic chloride ligand with other halides, such as Br or I. This process also affords binuclear complexes as mixtures of kinetically and thermodynamically controlled regioisomers. In CDCl3 solutions, we observed interconversion of kinetically and thermodynamically controlled regioisomers. The results of the DFT calculations revealed that in CHCl3 solution, each pair of the isomers exhibited two different types of chalcogen bonding such as S···X or S···N; the presence of CBs for two complexes in the solid state was also proven through X-ray crystallographic study. Based on the combined experimental and theoretical data, it could be concluded that thermodynamic favorability for the formation of thermodynamically controlled regioisomers increases in the Cl < Br ≈ I row and correlate well with the energy difference between S···N and S···X (X = Cl, Br, I) chalcogen bonds in kinetically and thermodynamically controlled products. This means that it is possible to change the structure of metallocycles in binuclear diaminocarbene complexes by simply replacing one halide ligand with another.
Cardiac Inflammation in Adult-Onset Genetic Dilated Cardiomyopathy
Authors: Sikking Maurits A. , Stroeks Sophie L. V. M. , Henkens Michiel T. H. M. , Venner Max F. G. H. M. , Li Xiaofei , Heymans Stephane R. B. , Hazebroek Mark R. , Verdonschot Job A. J.
Published: June 2023

Dilated cardiomyopathy (DCM) has a genetic cause in up to 40% of cases, with differences in disease penetrance and clinical presentation, due to different exogeneous triggers and implicated genes. Cardiac inflammation can be the consequence of an exogeneous trigger, subsequently unveiling a phenotype. The study aimed to determine cardiac inflammation in a cohort of genetic DCM patients and investigate whether it associated with a younger disease onset. The study included 113 DCM patients with a genetic etiology, of which 17 had cardiac inflammation as diagnosed in an endomyocardial biopsy. They had a significant increased cardiac infiltration of white blood, cytotoxic T, and T-helper cells (p < 0.05). Disease expression was at a younger age in those patients with cardiac inflammation, compared to those without inflammation (p = 0.015; 50 years (interquartile range (IQR) 42–53) versus 53 years (IQR 46–61). However, cardiac inflammation was not associated with a higher incidence of all-cause mortality, heart failure hospitalization, or life-threatening arrhythmias (hazard ratio 0.85 [0.35–2.07], p = 0.74). Cardiac inflammation is associated with an earlier disease onset in patients with genetic DCM. This might indicate that myocarditis is an exogeneous trigger unveiling a phenotype at a younger age in patients with a genetic susceptibility, or that cardiac inflammation resembles a ‘hot-phase’ of early-onset disease.
Correction: Jansa et al. Hospitalisation Is Prognostic of Survival in Chronic Thromboembolic Pulmonary Hypertension. J. Clin. Med. 2022, 11, 6189
Authors: Jansa Pavel , Ambrož David , Aschermann Michael , Černý Vladimír , Dytrych Vladimír , Heller Samuel , Kunstýř Jan , Lindner Jaroslav , Linhart Aleš , Nižnanský Matúš , Pad’our Michal , Prskavec Tomáš , Širanec Michal , Edwards Susan , Gressin Virginie , Kuhn Matyáš , Di Scala Lilla
Published: June 2023

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Outcome of Gastric Bypass Surgery on Patients with Polycystic Ovary Syndrome: A Review
Authors: Ghobrial Stefan , Ott Johannes , Steininger Johanna , Dewailly Didier , Prager Gerhard
Published: June 2023

Polycystic ovary syndrome (PCOS), the most common endocrine disorder in women of reproductive age, is associated with obesity. The most effective method to achieve and maintain long-term weight loss is by the Roux-en-Y gastric bypass (RYGB). In this review, an overview about metabolic and PCOS-specific outcomes after RYGB in obese PCOS women is provided. The RYGB leads to an adequate excess weight loss and reduction in BMI in this patient population. Testosterone levels decline significantly at 6- and 12-months follow-up, as does the incidence of hirsutism and cycle irregularities. Data about fertility are scarce in this patient population. In conclusion, RYGB surgery seems to be an efficient treatment option for obese PCOS patients and leads to weight loss and improvements in metabolic parameters as well as in an improvement of PCOS-specific characteristics. However, larger prospective studies are warranted, which include all PCOS-specific outcome data in one patient population at the same time.
Prognostic Impact of Psoas Muscle Mass Index following Trans-Catheter Aortic Valve Replacement
Authors: Imamura Teruhiko , Fujioka Hayato , Ushijima Ryuichi , Sobajima Mitsuo , Fukuda Nobuyuki , Ueno Hiroshi , Kinugawa Koichiro
Published: June 2023

Background: Psoas muscle mass is a recently featured index of sarcopenia, which has a negative prognostic impact in patients with a variety of diseases. We investigated the prognostic impact of baseline psoas muscle mass in patients receiving a trans-catheter aortic valve replacement (TAVR). Methods: Patients who received TAVR at our center between 2015 and 2022 were included. Patients received computer tomography imaging upon admission as an institutional protocol, and psoas muscle mass was measured, which was indexed by body surface area. Patients were followed for four years or until January 2023. The prognostic impact of psoas muscle mass index on 4-year mortality following index discharge was evaluated. Results: A total of 322 patients (85 years, 95 male) were included. Median psoas muscle mass index at baseline was 10.9 (9.0, 13.5) × 10 cm3/m2. A lower psoas muscle mass index tended to be associated with several index of malnutrition and sarcopenia. A psoas muscle mass index was independently associated with 4-year mortality with an adjusted hazard ratio of 0.88 (95% confidence interval 0.79–0.99, p = 0.044). Patients with lower psoas muscle mass index (below the statistically calculated cutoff of 10.7 × 10 cm3/m2, N = 152) had significantly higher cumulative 4-year mortality compared with others (32% versus 13%, p = 0.008). Conclusions: A lower psoas muscle mass index, which is a recently featured objective marker of sarcopenia, was associated with mid-term mortality following TAVR in the elderly cohort with severe aortic stenosis. The measurement of psoas muscle mass index prior to TAVR could have clinical implications for shared decision-making among patients, their relatives, and clinicians.
Portal Vein Thrombosis in Liver Transplantation: A Retrospective Cohort Study
Authors: Barrera-Lozano Luis Manuel , Ramírez-Arbeláez Jaime Alberto , Muñoz Cristian Leonardo , Becerra Jorge Andrés , Toro Luis Guillermo , Ardila Carlos M.
Published: June 2023

Portal vein thrombosis was considered a contraindication for liver transplantation. This study analyzes the perioperative complications and survival of liver transplant patients with portal vein thrombosis (PVT). A retrospective observational cohort study of liver transplant patients was conducted. The outcomes were early mortality (30 days) and patient survival. A total of 201 liver transplant patients were identified and 34 (17%) patients with PVT were found. The most frequent extension of thrombosis was Yerdel 1 (58.8%), and a portosystemic shunt was identified in 23 (68%) patients. Eleven patients (33%) presented any early vascular complication, PVT being the most frequent (12%). The multivariate regression analysis showed a statistically significant association between PVT and early complications (OR = 3.3, 95% confidence interval 1.4–7.7; p = 0.006). Moreover, early mortality was observed in eight patients (24%), of which two (5.9%) presented Yerdel 2. For Yerdel 1, patient survival according to the extent of thrombosis was 75% at 1 year and 3 years, while for Yerdel 2, it was 65% at 1 year, and 50% at 3 years (p = 0.04). Portal vein thrombosis significantly influenced early vascular complications. Furthermore, portal vein thrombosis Yerdel 2 or higher impacts the survival of liver grafts in the short and long term.
The Efficacy of Electromagnetic Diathermy for the Treatment of Musculoskeletal Disorders: A Systematic Review with Meta-Analysis
Authors: Pollet Joel , Ranica Giorgia , Pedersini Paolo , Lazzarini Stefano G. , Pancera Simone , Buraschi Riccardo
Published: June 2023

OBJECTIVE: This study aims to establish the effect of electromagnetic diathermy therapies (e.g., shortwave, microwave, capacitive resistive electric transfer) on pain, function, and quality of life in treating musculoskeletal disorders. METHODS: We conducted a systematic review according to the PRISMA statement and Cochrane Handbook 6.3. The protocol has been registered in PROSPERO: CRD42021239466. The search was conducted in PubMed, PEDro, CENTRAL, EMBASE, and CINAHL. RESULTS: We retrieved 13,323 records; 68 studies were included. Many pathologies were treated with diathermy against placebo, as a standalone intervention or alongside other therapies. Most of the pooled studies did not show significant improvements in the primary outcomes. While the analysis of single studies shows several significant results in favour of diathermy, all comparisons considered had a GRADE quality of evidence between low and very low. CONCLUSIONS: The included studies show controversial results. Most of the pooled studies present very low quality of evidence and no significant results, while single studies have significant results with a slightly higher quality of evidence (low), highlighting a critical lack of evidence in the field. The results did not support the adoption of diathermy in a clinical context, preferring therapies supported by evidence.
A Case Study of 21st Century Cognitive, Social and Emotional Competencies Using Online-Learning
Authors: Maoulida Haïfat , Madhukar Manisha , Celume Macarena-Paz
Published: June 2023

Based on the conceptualisation of the 21st Century Competencies Framework from the Center for Curriculum Redesign (CCR) we developed an online program to enable school-age students to increase their level on several social-emotional competencies. BE organized is a program that aims to help students to better organize themselves to be more efficient in today’s and tomorrow’s world. To do so, 12 individual sessions were designed to develop 4 out of the twelve 21st century competencies: Critical Thinking, Mindfulness, Resilience and Metacognition; collective sessions (action lab) to develop others such as Creativity. We used a mixed methodology, i.e., quantitative (two questionnaires) and qualitative (reflective questions) evaluation to test whether the targeted competencies have been developed during this program. Preliminary results (since it involves only a small number of participants, n = 27) partially confirm our hypotheses. Both qualitative and quantitative data show a development of critical thinking; the cross-sectional results are more mixed for the other three targeted competencies. Moreover, some other competencies, such as Creativity and Growth Mindset, seem to be developed during this program. However, it is difficult to determine whether it is the group and/or individual sessions that are responsible for these non-targeted competencies development. These results will be discussed in relation to the youth literature on 21st century competency and the broader literature on socio-emotional learning (SEL) and/or emotional intelligence (EI).
Theoretical Analysis Method for Roll Motion of Popup Data Communication Beacons
Authors: Song Yuanjie , Chi Haoyuan , Yu Liang , Wang Chen , Tian Chuan
Published: June 2023

The popup data communication beacon (PDCB) can send data to the shore and ships through the BeiDou navigation satellite system (BDS) when it surfaces. The data can be collected by a deep-sea landing vehicle (DSLV) and transmitted using a magnetic induction coil. PDCBs can reduce the cost of DSLV recovery and redeployment. Whether the data can be successfully sent mainly depends on the outlet height and roll angle of the PDCB. Thus, accurately assessing the effect of the roll angle on data transmission is crucial. In this study, first, the differential equation of roll motion was preliminarily established using the small-amplitude wave theory along with the shape characteristics of the PDCB. Next, the nonlinear term of the recovery moment was processed using the Linz Ted Poincaré method. Then, the wave current force was analyzed using the Morrison theoretical formula along with an additional inertia moment calculation formula that is suitable for slender cylindrical small buoys. Finally, the theoretical calculation results were verified using the computational fluid dynamics (CFD) method and pool test. The roll angle error of the theoretical calculation was within 5%. Thus, the heave and roll response of PDCBs can be evaluated using theoretical calculation methods. The proposed calculation formula of additional inertia moment has guiding significance for the further optimization of the structure.
Multiscale Analysis and Prediction of Sea Level in the Northern South China Sea Based on Tide Gauge and Satellite Data
Authors: Yang Yilin , Cheng Qiuming , Tsou Jin-Yeu , Wong Ka-Po , Men Yanzhuo , Zhang Yuanzhi
Published: June 2023

Under the influence of global warming, the problem of sea-level rise is becoming increasingly prominent. The northern part of the South China Sea (SCS) is low lying, with intense economic development, and densely populated. These characteristics make the region extremely sensitive to the consequences of rising sea levels. This study aims to reveal the trends of sea-level changes in the northern SCS and provide scientific insights into the potential flooding risks in low-lying areas. To achieve this, the Ensemble Empirical Mode Decomposition (EEMD) method is used to analyze the water level time series data from three tide gauges along the coast of Hong Kong. This analysis reveals the multidimensional change characteristics and response mechanisms of the sea level in the SCS. The findings reveal distinct seasonal, interannual, decadal, and interdecadal variations in sea-level changes. Furthermore, we explore the impact of the El Niño-Southern Oscillation (ENSO) on sea-level changes in the study area, finding a 6-month lagged correlation between the sea level and ENSO. Spatially, the rate of sea-level change is faster in nearshore areas than in the open ocean and higher in the northern regions than in the southern regions. The Multifractal Detrended Fluctuation Analysis (MF-DFA) method is employed to analyze the sea-level change time series, revealing long-range correlations and multifractal characteristics. In addition, we propose a sea-level prediction method that combines EEMD with Long Short-Term Memory (LSTM) neural networks and conducts empirical research on sea-level changes in the northern South China Sea. The results indicate that the EEMD-LSTM model outperforms the standalone LSTM model in terms of predictive accuracy, effectively eliminating noise from signals and providing a valuable reference. In summary, this research delves into the multiscale characteristics and influencing factors of sea-level changes in the northern SCS, proposing an improved sea-level prediction method that integrates EEMD and LSTM. The findings lay the groundwork for evaluating the risks of sea-level rise in low-lying regions of the northern SCS and inform future response strategies.
Staphylococcus aureus and Coagulase-Negative Staphylococci from Bloodstream Infections: Frequency of Occurrence and Antimicrobial Resistance, 2018–2021
Authors: Serra Nicola , Di Carlo Paola , Andriolo Maria , Mazzola Giovanni , Diprima Elena , Rea Teresa , Anastasia Antonio , Fasciana Teresa Maria Assunta , Pipitò Luca , Capra Giuseppina , Giammanco Anna , Cascio Antonio
Published: June 2023

Background: The abuse of antibiotics during the SARS-CoV-2 pandemic might have disrupted efforts to curb the further development and spread of the antimicrobial resistance of Staphylococcus aureus infection and Staphylococcus spp. coagulase-negative (CoNS) agents of nosocomial bloodstream infections (NBSIs). The purpose of our work was to study the resistance patterns of Staphylococcus aureus and CoNS through the analysis of blood cultures in hospitalized SARS-CoV-2-positive and SARS-CoV-2-negative patients (pts.). Materials and methods: During the period January 2018–June 2021, a retrospective case–control study was performed on blood cultures positive for Staphylococcus spp. detected in 177 adult pts. (≥18 years old) hospitalized for >48 hours at Sant’Elia Hospital, Caltanissetta. Results: Staphylococcus aureus was isolated in 33.9% of blood culture samples, and among CoNS, the most frequent strains were Staphylococcus capitis (18.6%) and Staphylococcus hominis (18.1%). Patients aged ≥ 65 years, with a greater number of males, comprised the SARS-CoV-2-negative pts. (71.8% vs. 52.2%, p = 0.0154). Among the SARS-CoV-2-positive patients, the significant resistance of Staphylococcus aureus was only observed for erythromycin (57.1%). The oxacillin resistance of Staphylococcus capitis was higher in SARS-CoV-2-positive than in negative pts. (90% and 78.3%, respectively). Comparing the two groups, we found an increase in resistance in SARS-CoV-2-negative patients for the following antibiotics: gentamicin for Staphylococcus aureus (p = 0.007), clindamycin and erythromycin (p = 0.012) for Staphylococcus hominis and oxacillin and rifampicin for Staphylococcus haemoliticus (p = 0.012). Conclusions: Our study confirms the relevance of oxacillin-resistant Staphylococcus aureus in being responsible for bloodstream infection and draws attention to highly oxacillin-resistant CoNS such as Staphylococcus capitis. The presence of resistant strains of CoNS in hospitals can be worrying, as it limits treatment options and worsens outcomes. The Infection Control Committee (ICC) recommends new treatment strategies to decrease colonization and infections. As part of the implementation of a bloodstream infection prevention program, the authors encourage the introduction of a report on the antimicrobial resistance of hospital bacteremia due to CoNS.
The Intricate Interplay between Cancer Stem Cells and Oncogenic miRNAs in Breast Cancer Progression and Metastasis
Authors: Tsintarakis Antonis , Papalouka Chara , Kontarini Christina , Zoumpourlis Panagiotis , Karakostis Konstantinos , Adamaki Maria , Zoumpourlis Vassilis
Published: June 2023

Complex signaling interactions between cancer cells and their microenvironments drive the clonal selection of cancer cells. Opposing forces of antitumor and tumorigenic potential regulate the survival of the fittest clones, while key genetic and epigenetic alterations in healthy cells force them to transform, overcome cell senescence, and proliferate in an uncontrolled manner. Both clinical samples and cancer cell lines provide researchers with an insight into the complex structure and hierarchy of cancer. Intratumor heterogeneity allows for multiple cancer cell subpopulations to simultaneously coexist within tumors. One category of these cancer cell subpopulations is cancer stem cells (CSCs), which possess stem-like characteristics and are not easily detectable. In the case of breast cancer, which is the most prevalent cancer type among females, such subpopulations of cells have been isolated and characterized via specific stem cell markers. These stem-like cells, known as breast cancer stem cells (BCSCs), have been linked to major events during tumorigenesis including invasion, metastasis and patient relapse following conventional therapies. Complex signaling circuitries seem to regulate the stemness and phenotypic plasticity of BCSCs along with their differentiation, evasion of immunosurveillance, invasiveness and metastatic potential. Within these complex circuitries, new key players begin to arise, with one of them being a category of small non-coding RNAs, known as miRNAs. Here, we review the importance of oncogenic miRNAs in the regulation of CSCs during breast cancer formation, promotion and metastasis, in order to highlight their anticipated usage as diagnostic and prognostic tools in the context of patient stratification and precision medicine.
Regeneration and Recovery after Acetaminophen Hepatotoxicity
Authors: Bhushan Bharat , Apte Udayan
Published: June 2023

Liver regeneration is a compensatory response to tissue injury and loss. It is known that liver regeneration plays a crucial role in recovery following acetaminophen (APAP)-induced hepatotoxicity, which is the major cause of acute liver failure (ALF) in the US. Regeneration increases proportional to the extent of liver injury upon APAP overdose, ultimately leading to regression of injury and spontaneous recovery in most cases. However, severe APAP overdose results in impaired liver regeneration and unchecked progression of liver injury, leading to failed recovery and mortality. Inter-communication between various cell types in the liver is important for effective regenerative response following APAP hepatotoxicity. Various non-parenchymal cells such macrophages, stellate cells, and endothelial cells produce mediators crucial for proliferation of hepatocytes. Liver regeneration is orchestrated by synchronized actions of several proliferative signaling pathways involving numerous kinases, nuclear receptors, transcription factors, transcriptional co-activators, which are activated by cytokines, growth factors, and endobiotics. Overt activation of anti-proliferative signaling pathways causes cell-cycle arrest and impaired liver regeneration after severe APAP overdose. Stimulating liver regeneration by activating proliferating signaling and suppressing anti-proliferative signaling in liver can prove to be important in developing novel therapeutics for APAP-induced ALF.