bluuebunny/crossref_metadata_2025 huggingface dataset:
The Toddler Autism Symptom Inventory: Use in diagnostic evaluations of toddlers
Authors: Coulter Kirsty L , Barton Marianne L , Boorstein Hilary , Cordeaux Cara , Dumont-Mathieu Thyde , Haisley Lauren , Herlihy Lauren , Jashar Dasal Tenzin , Robins Diana L , Stone Wendy L , Fein Deborah A
Published: June 2021

Although symptoms of autism are present early in life and early diagnosis can lead to better outcomes, there is a dearth of validated caregiver-report interviews designed for children under the age of 3 years. We developed the Toddler Autism Symptom Inventory, a semi-structured interview designed to assess the presence and absence of skills and symptoms in children aged 12–36 months. Reliability and validity of items and a cutoff score for likelihood of autism spectrum disorder were established. Specificity and sensitivity of this cutoff were confirmed with a cross-validation sample. The Toddler Autism Symptom Inventory effectively identified most children with autism without excessive false positives. The Toddler Autism Symptom Inventory is a developmentally appropriate caregiver interview for use in diagnostic evaluations of children under age 3 years that offers clearly operationalized diagnostic criteria and a cutoff for autism likelihood for very young children.

Lay abstract

Determining whether a young child has an autism spectrum disorder requires direct observation of the child and caregiver report of the child’s everyday behaviors. There are few interviews for parents that are specifically designed for children under 3 years of age. The Toddler Autism Symptom Inventory is a new interview that asks caregivers of children age 12–36 months about symptoms of possible autism spectrum disorder. The Toddler Autism Symptom Inventory uses a cutoff score to indicate likelihood for autism spectrum disorder; this cutoff score appears to accurately identify most children who are diagnosed with autism spectrum disorder without identifying too many who do not have autism spectrum disorder. The Toddler Autism Symptom Inventory interview can help clinicians to determine whether a young child shows symptoms suggestive of an autism spectrum disorder.
Preschool teachers’ selection of social communication interventions for children with autism: An application of the theory of planned behavior
Authors: Hugh Maria L , Johnson LeAnne D , Cook Clayton
Published: June 2021

Early Childhood Special Education teachers select practices to use to promote social communication development for their students with autism spectrum disorder. Understanding what evidence-based practices teachers select and why can inform the development of dissemination and implementation supports at the critical Adoption-Decision stage of implementation. The researchers used discrete choice analysis to examine and test the effects of Early Childhood Special Education teachers’ beliefs (self-efficacy, attitude, subjective norms; Theory of Planned Behavior) about key evidence-based practices on their practice selections. To assess malleable determinants of practice selection, this study addressed these aims: (1) assess Early Childhood Special Education teachers’ beliefs (attitudes, subjective norms, and self-efficacy) about key evidence-based practices, (2) describe which evidence-based practices teachers selected, and (3) examine whether teachers’ beliefs predicted practice selections. A web-based survey gathered responses from 222 Early Childhood Special Education teachers. Participants had the most favorable beliefs about naturalistic intervention and the least about discrete trial teaching and scripting. These beliefs aligned with the frequencies with which these practices were selected, and teachers’ beliefs predicted which practices they selected. Together, these findings suggest that beliefs serve as determinants of Early Childhood Special Education teachers’ practice selections that can be acted on to tailor pre-implementation supports and improve implementation from the start.

Lay abstract

Preschool special educators’ are more likely to choose an educational practice to teach a young child with autism a social communication skill if they have positive beliefs about it. We asked preschool special educators to read a description of an autistic student and their social communication goal and imagine they were the student’s teacher. We then asked them to pick one of five practices to teach the student. We also asked them questions to understand their attitudes about, confidence in their ability to use, and their perception of their coworkers’ support of each practice. There are many research-based practices that a teacher could use to help children learn, and preschool teachers often make these decisions for their students. Teachers’ beliefs varied in how supportive they were of each practice, and research shows people are more likely to do something that their beliefs support. In this study, they had more supportive beliefs and were more likely to use some practices, like naturalistic intervention, than other practices, like discrete trial teaching. By knowing this, researchers can help teachers use practices that their beliefs support and help change teachers’ beliefs to be supportive of a practice they may need to use.
Societal multilingualism à la balkanique: The Montenegrin Velja Gorana and beyond
Authors: Morozova Maria S , Rusakov Alexander Yu
Published: June 2021

Aims and objectives:

The goal of the article is to explore the properties of bilingual and multilingual situations with balanced, or non-polyglossic, relationships between languages in the modern Balkans. A comparison with non-balanced, or polyglossic, settings aims at determining the factors that support balanced bilingualism in one Balkan community and result in different dominance configurations in the others.

Methodology:

We focus, on the one hand, on the community’s inner diversity and the variation in linguistic competence among its individual members, showing how the differences of the various groups of speakers contribute to the maintenance of bilingualism in the community across several generations. On the other hand, we consider speech community as a unit of study and see how factors such as geographical location, size, and presence or absence of ties with the surrounding region, contribute to the loss or maintenance of community bilingualism and multilingualism in different parts of the Balkan linguistic area.

Findings:

The main case that is scrutinized is the situation of “balanced language contact” in a small-scale village community of Velja Gorana (Montenegro). Its analysis is based on field observations made in the bilingual families of Velja Gorana in 2013 to 2015. Besides, we overview a range of Balkan bilingual and multilingual communities, based on the existing literature, and analyse the patterns of (socio)linguistic dominance of the languages spoken in each community.

Originality:

The article provides a description of a balanced situation in the modern Balkans and offers a model for understanding of the Balkan bilingualism and multilingualism, taking into consideration several factors interacting at different levels, from individual speaker to the speech community.

Implications:

The proposed model explains the diversity of bilingual and multilingual situations of the modern Balkans. This enables us to make predictions about the ways of development of these situations and suggest how the past Balkan situations could have been organized.
Predicting the effect of weathering and corrosion on the bond properties of bamboo- and steel-reinforced cement-stabilized rammed earth blocks
Authors: Raavi Satya Sai Deep , Tripura Deb Dulal
Published: June 2021

In this article, the effect of weathering and corrosion on the bond properties of bamboo- and steel-reinforced cement-stabilized rammed earth blocks was investigated. The treated, untreated bamboo and steel reinforcement types were considered under regular and weathered categories. Reinforcement of 8 mm, 10 mm and 12 mm diameters were used along with 10% of cement as stabilizer. A total of 90 reinforced cement-stabilized rammed earth blocks were prepared and tested for bond strength. The investigation shows that the bond force and bond strength of all the blocks reduced due to weathering and corrosion of reinforcement. In case of blocks with bamboo reinforcement only, a minor reduction in bond properties (bond force and bond strength) was identified, but in case of blocks with steel reinforcement, a major reduction in bond properties was identified. All the blocks failed by either lateral splitting, pullout or pullout along with lateral splitting. However, the pullout failure was observed only in the blocks with weathered or corroded reinforcement, making it clear that the mode of failure was influenced by the type and physical condition of the reinforcement. Based on the results obtained, it was not advisable to use of corroded steel (CS) bars as reinforcement in rammed earth. However, considering the bond properties, treated bamboo can be a potential and economical alternative to CS. A series of statistical analysis was performed using the test data to predict the bond properties correlating perimeter, diameter, type and condition of reinforcement. The regression equations generated from statistical analysis represent a strong correlation between the actual and predicted values and can be used for predicting the bond properties of rammed earth accurately.
Psychological treatments for post-traumatic stress disorder, anxiety and depression following major physical civilian trauma: A systematic review and meta-analysis
Authors: Qureshi Arham , Dickenson Edward , Wall Peter
Published: June 2021

Introduction

Approximately 16% of the world’s burden of disease is attributable to traumatic injury. Psychological symptoms, including post-traumatic stress disorder (PTSD), are prevalent in this population and impact recovery from physical injury. Nevertheless, mental health has not been considered to the same degree as physical health. Psychological interventions are used widely as treatments for PTSD.

Methods

Systematic searches of computerised databases were conducted. Randomised controlled trials of psychological treatments for PTSD following major physical civilian trauma were included. The main outcome measure was clinician-assessed symptoms of PTSD (CAPS), with findings for anxiety and depression also reported. Included studies data were extracted and entered using RevMan 5.3 software. Quality assessments were performed, and data were analysed for summary effects.

Results

10 studies were included. With regard to CAPS <6 months, individual CBT did significantly better than usual care/wait list (SMD (95% CI) = −1.24 [−1.82, −0.67]) and non-CBT treatments (SMD (95% CI) = −1.32 [−2.64, −0.04]). Non-CBT treatments were not significantly better than usual care/wait list (SMD (95% CI) −1.40 [−2.91, 0.11]). CBT was superior to usual care/wait list for reducing depressive (SMD (95% CI) −0.67 [−0.98, −0.37]) and anxiety (SMD (95% CI) −0.70 [−1.22, −0.18]) symptoms both in the shorter and longer term.

Conclusion

Individual CBT was superior to wait list/usual care, and there was limited evidence for non-CBT treatments in reducing clinician and self-rated PTSD, depressive and anxiety symptoms in the shorter term; however, the latter comparison was based on few studies with small sample sizes. Longer-term effects of treatments remain uncertain. There is a need for adequately powered RCTs investigating PTSD treatments following major physical civilian trauma in the longer term. There was considerable heterogeneity in the studies, so care must be taken in interpreting the results of this review.
Current Scottish Land Reform and Reclaiming the Commons: Building Community Resilience
Authors: Danson Mike , Burnett Kathryn A.
Published: June 2021

Scotland has been addressing the highest European concentrations of land ownership through land reform legislation, encouraging communities to buy out the lairds. Collective efforts to take ownership of the commons are explored through application of theories on governance, regional development and institutions. Experiences of Inner and Outer Hebridean islanders under private and then community ownership, paying special attention to the case of the Isle of Eigg, are considered. Their collective tenacity, flexibility and confidence in securing a community future despite continuing challenges to remote small island living are offered as keystone examples from Scotland of small island enterprise, social development and collective community actions.
Comfort Equals Nurturing: Young People Talk About Mental Health Ward Design
Authors: Hutton Alison , Wilson Rhonda , Foureur Maralyn
Published: June 2021

Aim:

The aim of this research was to obtain user perspectives of adolescent patients to inform the redesign of an adolescent mental health inpatient facility ward unit in regional Australia.

Background:

It is well established that the consumer will use and understand unit space differently than the health professional, because of their unique positioning and depth of experience. The participation of young people in the design of ward environments and programs is essential.

Methods:

A combination of focus groups and interviews was undertaken with eight participants.

Results:

This study found that, in its current form, the unit revolves around routines, tasks, and patient safety, which in turn are driven by secure spaces and surveillance of the patients. Due to these routines and tasks, the aspects of individualized patient care and personal impetus and comfort are lost. Adolescent patients are able to identify solutions to enhance their hospital stay in the mental health context without being excessive and unrealistic in their suggestions.

Conclusions:

When designing an adolescent unit, there needs to be some consideration as to how the group using the unit understands space. Through their role and depth of hospital experience, the adolescent patient will use and understand unit space differently from that of the health professional whose role is a worker. Therefore, it is necessary to gain the perspectives of adolescent patients for efficient and effective functioning of such an environment and to suit the needs of those it is “built for,” the adolescent patient.
Luxury Branding in the Hospitality Industry: The Impact of Employee’s Luxury Appearance and Elitism Attitude
Authors: Kim Kawon , Baker Melissa A.
Published: June 2021

Some of the luxury consumption literature suggests that luxury consumption is a beneficial social signal for the actor which facilitates social interaction. However, a different stream of recent research suggests that luxury consumption bears social costs to the actor. In the employee–customer interaction context, wearing luxury brands can either benefit or backfire for the employee depending on the situation whether luxury status or warmth is necessary. Based on the gaps in the literature, this study examines the impact of employee conspicuous cues by utilizing luxury consumption and elitism attitude on employee–customer rapport and behavioral intentions. The study results show that employees wearing luxury brands increase customers’ perceived impression management toward the employee. Such perception is strengthened when employees show an elitism attitude. In addition, when employees wear luxury brands, customers are more likely to build rapport with employees when they show a democratic attitude, as they perceive the employees are less likely to involve in impression management than showing an elitism attitude. The results build upon the luxury hospitality literature, aesthetic labor, impression management, and rapport literature.
Debate on the linkages between large-scale agriculture and farmers food security: Examples from Ethiopia
Authors: Abebe Gezahegn
Published: June 2021

This article critically contributes to the debate on private large-scale agriculture for export crop production as a means to address the problem of food security in Ethiopia. The reviewed literature was identified by combining relevant keywords. The consumer price index and rainfall pattern, food gaps and affected population were calculated and presented using Microsoft Excel software. The paper argues that although private large-scale agriculture has opportunities in terms of employment generation, income and national-level growth, it poses risks to small-scale farmers’ food security. Loss of access to agricultural land and exposure to market risks endanger their food and livelihood security. The analysis suggests that both large-scale and small-scale agriculture is not a viable means to effectively reduce Ethiopia’s complex food insecurity and hunger. Improving food production for local consumption through transforming small-scale farms to medium-sized labour-intensive farms coupled with investment in manufacturing and building social protection through the adoption of well-intentioned state-directed development interventions that care about the well-being of individuals and households is needed.
Cartilage Biomarkers Coll2-1 and Coll2-1NO2 Are Associated with Knee OA MRI Features and Are Helpful in Identifying Patients at Risk of Disease Worsening
Authors: Hick Anne-Christine , Malaise Michel , Loeuille Damien , Conrozier Thierry , Maugars Yves , Pelousse Franz , Tits Cédric , Henrotin Yves
Published: June 2021

Objective:

To assess the cross-sectional association between serum levels of Coll2-1 and Coll2-1NO2, two cartilage degradation biomarkers; the burden of magnetic resonance imaging (MRI) features and clinical outcomes; and to evaluate the predictive value of these biomarkers on progression.

Design:

A total of 121 subjects with knee osteoarthritis (OA) were followed during 1 year with pain, function, and MRI assessment (PRODIGE study). Type II collagen-specific biomarker Coll2-1 and its nitrated form Coll2-1NO2 were directly measured in serum using immunoassays at baseline and after 3-, 6-, and 12-month follow-up.

Results:

Serum Coll2-1 and Coll2-1NO2 were correlated with several baseline knee features quantified with Whole-Organ Magnetic Resonance Imaging Score (WORMS). Coll2-1 was significantly correlated with periarticular cysts/bursitis (ρ = 0.29, P < 0.01), subarticular bone attrition (ρ = 0.25, P = 0.01), subarticular cysts (ρ = 0.24, P = 0.02), and articular cartilage integrity (ρ = 0.23, P = 0.03) WORMS subscores for the whole joint as well as with the medial femorotibial joint sum score (ρ = 0.26, P = 0.01) and medial femorotibial joint cartilage (ρ = 0.23, P = 0.02). Coll2-1NO2 correlated with WORMS total score (ρ = 0.23, P = 0.02), WORMS scores in the patellofemoral (ρ = 0.23, P = 0.02) and medial femorotibial compartments (ρ = 0.21, P = 0.03), with osteophytes scores (ρ = 0.27, P < 0.01), subarticular cysts (ρ = 0.24, P = 0.019), and intraarticular loose bodies (ρ = 0.27, P = 0.007). Baseline Coll2-1NO2 was higher in subjects with a pain worsening (426.4 pg/mL [278.04-566.95]) as compared to non-progressors (306.84 pg/mL [200.37-427.84]) over 1 year (AUC = 0.655, P = 0.015).

Conclusion:

Serum cartilage biomarkers Coll2-1 and Coll2-1NO2 are associated with several knee OA features quantified with WORMS. Our study also shows that the baseline value of Coll2-1NO2 is positively associated with pain worsening.
Real-world discontinuation rate of teriflunomide and dimethyl fumarate in multiple sclerosis
Authors: Norborg Hilde , Riise Trond , Myhr Kjell-Morten , Grytten Nina , Wergeland Stig
Published: June 2021

Background

For patients with MS, medication switches increase the risk of disease reactivation.

Objective

Compare discontinuation rates due to treatment failure or side effects between teriflunomide and dimethyl fumarate, and investigate clinical variables affecting discontinuation rates.

Methods

All patients who received teriflunomide or dimethyl fumarate at Haukeland University Hospital from 2013 until 2018 were identified. Clinical and demographic variables were extracted from the Norwegian MS Registry. Cause-specific Cox regression models estimated the rate of discontinuation due to treatment failure or side effects.

Results

We included 354 patients treated with either dimethyl fumarate ( n = 185) or teriflunomide ( n = 169). We found 38% lower risk of discontinuation because of treatment failure for patients using dimethyl fumarate compared to teriflunomide ( p < 0.05). In a treatment-naive subgroup ( n = 183), we found a 38% reduced risk of discontinuation for any reason among patients using dimethyl fumarate ( p < 0.05). There was no significant difference between treatment groups in discontinuation rate due to side effects, although more patients reported side effects when treated with dimethyl fumarate.

Conclusion

Our findings suggests that dimethyl fumarate has a lower risk of discontinuation because of treatment failure among both treatment-experienced and treatment-naive patients.
Multiple sclerosis in Kenya: Demographic and clinical characteristics of a registry cohort
Authors: Jamal Imran , Shah Jasmit , Mativo Peter , Hooker Juzar , Wallin Mitchell , Sokhi Dilraj Singh
Published: June 2021

Background

Multiple Sclerosis (MS) is the leading cause of non-traumatic neurological disability in young adults. There is limited literature regarding the burden of MS in sub-Saharan Africa (SSA).

Objective

To describe the demographic and clinical characteristics of patients with MS (PwMS) presenting to a tertiary referral hospital in Nairobi.

Methods

We conducted a retrospective descriptive study for PwMS presenting to Aga Khan University Hospital, Nairobi from 2008–2018.

Results

99 cases met the diagnostic criteria for MS with a male to female ratio of 1:4. Majority (68.7%) of PwMS were indigenous Africans with a mean age of onset of 30.7 years. Mean duration from symptom onset to first neuro-imaging was 5.04 years. Only 33% of patients had sensory symptoms at onset whereas 54.5% had vitamin D deficiency/insufficiency. Majority (79.5%) had relapsing remitting MS (RRMS) and 56.6% were initiated on disease modifying therapy (DMT). Only 21.2% of patients on DMT were non-compliant. Patients with RRMS were more likely to be initiated on DMT at our hospital (p < 0.001).

Conclusion

Clinical characteristics of these patients largely resemble those of other SSA cohorts and African American patients. There was a delay between symptom onset and neuroimaging. There were also issues with DMT compliance.
An Empirical Analysis of the Factors Affecting Farmer Satisfaction Under the China Link Policy
Authors: Gu Hanlong , Liu Yiying , Qian Fengkui , Wang Qiubing , Dong Xiuru
Published: June 2021

China’s urbanization has maintained a high growth rate for a long period of time, but the contradiction between urban and rural land use has become increasingly prominent. Link policy, promulgated in 2005 by the Chinese Central Government, made a positive attempt to promote the sustainable use of urban and rural land, which has attracted much attention. This article focuses on the implementation effects of this policy from the micro-perspective of farmers’ satisfaction and its underlying influence factors using a structural equation model (SEM) based on a cross-sectional dataset of 280 households surveyed in Chongqing city, China. The results show that the farmers’ satisfaction under the Link Policy is high, and most of the respondents are satisfied with the Link Policy at present. Furthermore, the farmers’ satisfaction is affected by their expectations, perceived value, and perceived quality. Among these factors, perceived quality has the strongest positive impact on farmers’ satisfaction, for which compensation equity under the Link Policy is most important to farmers. However, farmers’ expectations and perceived value have a negative impact on farmers’ satisfaction, which indicates that the higher the farmers’ expectations and perceived value, the lower their satisfaction. In addition, there is a strong positive correlation between farmer satisfaction and farmer trust, which means that the higher the level of farmer satisfaction, the more support the farmers will give to the government. These main results have heuristic implications for policymakers to improve the Link Policy, thereby enhancing the farmers’ satisfaction and thus achieving the goal of sustainable use between rural and urban land.
Social Isolation in a Population-Based Sample of Emerging Adults: Who Is on Their Own?
Authors: Simone Melissa , Loth Katie , Peterson Carol B. , Berge Jerica M. , Eisenberg Marla E. , Neumark-Sztainer Dianne
Published: June 2021

Purpose:

This study aimed to identify the associations between social isolation, social contextual factors, and behavioral and psychological health during emerging adulthood.

Methods:

Participants (n = 1,568) were drawn from EAT 2018 (mean age = 22.1 ± 2.0). Logistic regressions tested the associations between social isolation, contextual factors, and behavioral and psychological health.

Results:

Approximately 16% of the sample reported social isolation. Emerging adults with socially marginalized identities (e.g., racial/ethnic, socioeconomic), who were unemployed, and/or lived alone had higher odds of experiencing social isolation. Social isolation was associated with more risky health behaviors and lower self-esteem.

Discussion:

Emerging adults who were socially disadvantaged (e.g., unemployed) had greater risk of social isolation. Findings suggest that social isolation is more strongly linked with behavioral health than psychological health. Generally, findings suggest that access to community resources and areas wherein social connections can be formed may to be important for behavioral health in emerging adulthood.
Injury Profile Among Street Workout Practitioners
Authors: Ngo Jake K. , Solis-Urra Patricio , Sanchez-Martinez Javier
Published: June 2021

Background:

Street workout (SW) is an urban sport based on calisthenic exercises. Injury profiles and risk factors are widely explored in various sport disciplines. However, because of the lack of research in SW, injury profiles have not yet been established.

Purpose:

To investigate the characteristics and prevalence of injuries and variables that may increase these odds in SW practitioners.

Study Design:

Case-control study; Level of evidence, 4.

Methods:

An electronic survey was conducted between September 2018 and March 2019 in order to obtain practitioner information, physical activity level, training characteristics, and injury information of SW practitioners. Responses from 93 practitioners were eligible for analysis. Univariate and multivariate binary logistic regression analyses were used to examine the association among several variables and the presence of injury in the past 12 months based on odds ratios (ORs).

Results:

Overall, 62.4% of the 93 participants reported an injury in the previous 12 months, and tendinopathy was the most reported diagnosis (31.0% of injured practitioners). High injury frequency was reported in the shoulder (23.0%) and back (upper and mid) (18.4%). Overtraining was the most reported perception of cause of injury (56.9%). Most injured practitioners (20.7%) performed freestyle exercises before the injury, followed by upper body exercises. A history of previous injuries (OR, 4.078; P = .005) and the middle and higher tertiles of vigorous physical activity (OR, 4.370; P = .015 and OR, 5.634; P = .003, respectively) were associated with a higher odds of injury.

Conclusion:

In SW, the shoulder and back (upper and mid) were the most injured body parts, with tendinopathy being the most frequent diagnosis. Overtraining was typically reported as the perceived cause of injury, with freestyle and upper limb exercises the most performed exercises when injured. A history of previous injuries, along with vigorous physical activity, was found to augment the odds of injury. More research is needed to corroborate our findings, along with increased knowledge about SW practice and injuries.
Single-Stage Arthroscopic Cartilage Repair With Gel Scaffold and BMAC
Authors: Heng Christian Hwee Yee , Lee Yee Han Dave
Published: June 2021

Background:

Injectable scaffold augmentation has been gaining traction as a promising modality for single-stage cartilage repair. It involves the use of a biological scaffold that augments microfracture techniques by aiding in clot stabilization and maturation. The scaffold provides a matrix that helps with mesenchymal stem cell (MSC) retention and encourages differentiation along a chondrogenic lineage. Bone marrow aspirate concentrate (BMAC) has also been proposed as an alternative source of MSCs to microfracture, and it can potentially avoid the pitfalls of microfracture techniques.

Indications:

Injectable scaffold augmentation to microfracture techniques are recommended in lesions >4 cm, as long-term follow-up has shown increasing failure over time with microfracture alone.

Technique Description:

We describe a technique of autologous matrix-induced chondrogenesis using CartiFill, a porcine-derived type 1 collagen scaffold, combined with BMAC as a source of MSCs (avoiding the use of microfracture).

Results:

Injectable scaffold augmentation has been shown in recent studies to lead to better radiological fill, higher quality of histological repair, and better clinical outcomes as compared with microfracture alone. These injectable scaffolds have the versatility to be used on lesions which have traditionally been considered difficult to address, such as vertical or inverted lesions. Moreover, the use of scaffolds allows the repair to be further augmented with BMAC which provides a source of MSCs without the need to perform microfracture and perforate the subchondral bone.

Discussion/Conclusion:

Scaffold augmentation is a promising technique that improves upon the results of conventional microfracture repair by allowing augmentation with BMAC, as well as giving surgeons the versatility to apply the scaffold on vertical/inverted lesions. BMAC is also a viable alternative source of MSCs for cartilage repair.
Regional attenuation models in Central and Eastern North America using the NGA-East database
Authors: Bayless Jeff
Published: June 2021

The anelastic attenuation term found in ground motion prediction equations (GMPEs) represents the distance dependence of the effect of intrinsic and scattering attenuation on the wavefield as it propagates through the crust and contains the frequency-dependent quality factor, [Formula: see text], which is an inverse measure of the effective anelastic attenuation. In this work, regional estimates of [Formula: see text] in Central and Eastern North America (CENA) are developed using the NGA-East regionalization. The technique employed uses smoothed Fourier amplitude spectrum (FAS) data from well-recorded events in CENA as collected and processed by NGA-East. Regional [Formula: see text] is estimated using an assumption of average geometrical spreading applicable to the distance ranges considered. Corrections for the radiation pattern effect and for site response based on [Formula: see text] result in a small but statistically significant improvement to the residual analysis. Apparent [Formula: see text] estimates from multiple events are combined within each region to develop the regional models. Models are provided for three NGA-East regions: the Gulf Coast, Central North America, and the Appalachian Province. Consideration of the model uncertainties suggests that the latter two regions could be combined. There were not sufficient data to adequately constrain the model in the Atlantic Coastal Plain region. Tectonically stable regions are usually described by higher [Formula: see text] and weaker frequency dependence ([Formula: see text]), while active regions are typically characterized by lower [Formula: see text] and stronger frequency dependence, and the results are consistent with these expectations. Significantly different regional [Formula: see text] is found for events with data recorded in multiple regions, which supports the NGA-East regionalization. An inspection of two well-recorded events with data both in the Mississippi embayment and in southern Texas indicates that the Gulf Coast regionalization by Cramer in 2017 may be an improvement to that of NGA-East for anelastic attenuation. The [Formula: see text] models developed serve as epistemic uncertainty alternatives in CENA based on a literature review and a comparison with previously published models.
Genetic differentiation and restricted gene flow in rice landraces from Yunnan, China: effects of isolation-by-distance and isolation-by-environment
Authors: Cui Di , Tang Cuifeng , Lu Hongfeng , Li Jinmei , Ma Xiaoding , A Xinxiang , Han Bing , Yang Yayun , Dong Chao , Zhang Feifei , Dai Luyuan , Han Longzhi
Published: June 2021

Background

Understanding and identifying the factors responsible for genetic differentiation is of fundamental importance for efficient utilization and conservation of traditional rice landraces. In this study, we examined the spatial genetic differentiation of 594 individuals sampled from 28 locations in Yunnan Province, China, covering a wide geographic distribution and diverse growing conditions. All 594 accessions were studied using ten unlinked target genes and 48 microsatellite loci, and the representative 108 accessions from the whole collection were sampled for resequencing.

Results

The genetic diversity of rice landraces was quite different geographically and exhibited a geographical decline from south to north in Yunnan, China. Population structure revealed that the rice landraces could be clearly differentiated into japonica and indica groups, respectively. In each group, the rice accessions could be further differentiated corresponded to their geographic locations, including three subgroups from northern, southern and middle locations. We found more obvious internal geographic structure in the japonica group than in the indica group. In the japonica group, we found that genetic and phenotypic differentiation were strongly related to geographical distance, suggesting a pattern of isolation by distance (IBD); this relationship remained highly significant when we controlled for environmental effects, where the likelihood of gene flow is inversely proportional to the distance between locations. Moreover, the gene flow also followed patterns of isolation by environment (IBE) whereby gene flow rates are higher in similar environments. We detected 314 and 216 regions had been differentially selected between Jap-N and Jap-S, Ind-N and Ind-S, respectively, and thus referred to as selection signatures for different geographic subgroups. We also observed a number of significant and interesting associations between loci and environmental factors, which implies adaptation to local environment.

Conclusions

Our findings highlight the influence of geographical isolation and environmental heterogeneity on the pattern of the gene flow, and demonstrate that both geographical isolation and environment drives adaptive divergence play dominant roles in the genetic differentiation of the rice landraces in Yunnan, China as a result of limited dispersal.
Application of convolutional neural networks towards nuclei segmentation in localization-based super-resolution fluorescence microscopy images
Authors: Mela Christopher A. , Liu Yang
Published: June 2021

Background

Automated segmentation of nuclei in microscopic images has been conducted to enhance throughput in pathological diagnostics and biological research. Segmentation accuracy and speed has been significantly enhanced with the advent of convolutional neural networks. A barrier in the broad application of neural networks to nuclei segmentation is the necessity to train the network using a set of application specific images and image labels. Previous works have attempted to create broadly trained networks for universal nuclei segmentation; however, such networks do not work on all imaging modalities, and best results are still commonly found when the network is retrained on user specific data. Stochastic optical reconstruction microscopy (STORM) based super-resolution fluorescence microscopy has opened a new avenue to image nuclear architecture at nanoscale resolutions. Due to the large size and discontinuous features typical of super-resolution images, automatic nuclei segmentation can be difficult. In this study, we apply commonly used networks (Mask R-CNN and UNet architectures) towards the task of segmenting super-resolution images of nuclei. First, we assess whether networks broadly trained on conventional fluorescence microscopy datasets can accurately segment super-resolution images. Then, we compare the resultant segmentations with results obtained using networks trained directly on our super-resolution data. We next attempt to optimize and compare segmentation accuracy using three different neural network architectures.

Results

Results indicate that super-resolution images are not broadly compatible with neural networks trained on conventional bright-field or fluorescence microscopy images. When the networks were trained on super-resolution data, however, we attained nuclei segmentation accuracies (F1-Score) in excess of 0.8, comparable to past results found when conducting nuclei segmentation on conventional fluorescence microscopy images. Overall, we achieved the best results utilizing the Mask R-CNN architecture.

Conclusions

We found that convolutional neural networks are powerful tools capable of accurately and quickly segmenting localization-based super-resolution microscopy images of nuclei. While broadly trained and widely applicable segmentation algorithms are desirable for quick use with minimal input, optimal results are still found when the network is both trained and tested on visually similar images. We provide a set of Colab notebooks to disseminate the software into the broad scientific community (https://github.com/YangLiuLab/Super-Resolution-Nuclei-Segmentation).
Transcriptome analysis of scions grafted to potato rootstock for improving late blight resistance
Authors: Li Yuexin , Zhao Degang
Published: June 2021

Background

Late blight seriously threatens potato cultivation worldwide. The severe and widespread damage caused by the fungal pathogen can lead to drastic decreases in potato yield. Although grafting technology has been widely used to improve crop resistance, the effects of grafting on potato late blight resistance as well as the associated molecular mechanisms remain unclear. Therefore, we performed RNA transcriptome sequencing analysis and the late blight resistance testing of the scion when the potato late blight-resistant variety Qingshu 9 and the susceptible variety Favorita were used as the rootstock and scion, respectively, and vice versa. The objective of this study was to evaluate the influence of the rootstock on scion disease resistance and to clarify the related molecular mechanisms.

Results

A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that the expression levels of genes related to plant–pathogen interactions, plant mitogen-activated protein kinase (MAPK) signaling pathways, and plant hormone signal transduction pathways were significantly up-regulated in the scion when Qingshu 9 was used as the rootstock. Some of these genes encoded calcium-dependent protein kinases (CDPKs), chitin elicitor receptor kinases (CERKs), LRR receptor serine/threonine protein kinases (LRR-LRKs), NPR family proteins in the salicylic acid synthesis pathway, and MAPKs which were potato late blight response proteins. When Favorita was used as the rootstock, only a few genes of late blight response genes were upregulated in the scion of Qingshu 9. Grafted plants using resistant variety as rootstocks inoculated with P. infestans spores showed significant reductions in lesion size while no significant difference in lesion size was observed when susceptible variety was used as the rootstock. We also showed that this induction of disease resistance in scions, especially scions derived from susceptible potato varieties was mediated by the up-regulation of expression of genes involved in plant disease resistance in scions.

Conclusions

Our results showed that potato grafting using late blight resistant varieties as rootstocks could render or enhance resistance to late blight in scions derived from susceptible varieties via up-regulating the expression of disease resistant genes in scions. The results provide the basis for exploring the molecular mechanism underlying the effects of rootstocks on scion disease resistance.
An NADPH oxidase regulates carbon metabolism and the cell cycle during root nodule symbiosis in common bean (Phaseolus vulgaris)
Authors: Fonseca-García Citlali , Nava Noreide , Lara Miguel , Quinto Carmen
Published: June 2021

Background

Rhizobium–legume symbiosis is a specific, coordinated interaction that results in the formation of a root nodule, where biological nitrogen fixation occurs. NADPH oxidases, or Respiratory Burst Oxidase Homologs (RBOHs) in plants, are enzymes that generate superoxide (O2•−). Superoxide produces other reactive oxygen species (ROS); these ROS regulate different stages of mutualistic interactions. For example, changes in ROS levels are thought to induce ROS scavenging, cell wall remodeling, and changes in phytohormone homeostasis during symbiotic interactions. In common bean (Phaseolus vulgaris), PvRbohB plays a key role in the early stages of nodulation.

Results

In this study, to explore the role of PvRbohB in root nodule symbiosis, we analyzed transcriptomic data from the roots of common bean under control conditions (transgenic roots without construction) and roots with downregulated expression of PvRbohB (by RNA interference) non-inoculated and inoculated with R. tropici. Our results suggest that ROS produced by PvRBOHB play a central role in infection thread formation and nodule organogenesis through crosstalk with flavonoids, carbon metabolism, cell cycle regulation, and the plant hormones auxin and cytokinin during the early stages of this process.

Conclusions

Our findings provide important insight into the multiple roles of ROS in regulating rhizobia–legume symbiosis.
Outcomes after EVAR in females are similar to males
Authors: Tumer Naim Boran , Askin Goktan , Akkaya Bekir Bogachan , Civelek Isa , Unal Ertekin Utku , Iscan Hakki Zafer
Published: June 2021

Introduction

Women are less likely to develop infrarenal abdominal aortic aneurysm; however, when they do, it is almost always associated with challenging anatomy, more rapid aneurysmal growth rate and earlier rupture. Women generally have poorer outcomes following open aneurysm repair; and in this respect, the present study aims to evaluate if it is so after endovascular repair.

Methods

A retrospective analysis of our database was performed for patients underwent endovascular aneurysm repair (EVAR) between January 2013–March 2020. 249 elective EVAR patients were evaluated. Patients were categorized according to gender and 26 patients (10.4%) were female. Demographics and pre-peri-postoperative findings were compared. Propensity score matching (ratio 1:1) was performed to reduce selection bias.

Results

In the overall unmatched cohort, female population had more diabetes mellitus (p = 0.016) and hypertension (p = 0.005). However, coronary artery disease (p = 0.005) and coronary artery bypass grafting (p = 0.006) were more in male gender. Non-IFU implantation was higher in female group (38.5% vs. 11.5%, p = 0.025). After propensity matching, even though it was not statistically significant, early mortality for female gender was higher when compared to male gender (7.7% and 0%, respectively, p = 0.490). In the follow-up period, no difference in all-cause mortality, secondary interventions or complications have been observed between the genders.

Conclusion

Challenging anatomy and subsequently treated patients outside IFU may be the reasons for higher morbidity and mortality in women. However, despite these factors female and male patients revealed equivalent early and late results.
Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students
Authors: Munasinghe Thilini , Vidanapathirana Gihani , Kuthubdeen Shahlina , Ekanayake Asela , Angulmaduwa Sacheera , De Silva Kunchana , Subhasinghe Susan , Kalupahana Ruwani , Liyanapathirana Veranja , Ip Margaret
Published: June 2021

Background

Antibiotic Resistance is an imminent global public health threat. Antibiotic resistance emerged in healthcare settings and has now moved on to the community settings.

This study was conducted to identify the rates of asymptomatic colonization with selected antibiotic resistant organisms, (Methicillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli and Klebsiella spp and carbapenem resistant E.coli and Klebsiella spp) - among a group of university students in Sri Lanka. Identification of genetic determinants of MRSA and ESBL was an additional objective of the study.

Methods

A self - collected nasal swab and a peri-rectal swab collected after passing stools were obtained. Routine microbiological methods were used for the isolation S.aureus from the nasal swab and E.coli and Klebsiella species from the peri-rectal swab. Antibiotic sensitivity testing was performed as recommended by clinical and laboratory standard institute (CLSI).

Three (3) genes that are responsible for ESBL production; blaCTX-M, blaSHV, and blaTEM were tested using previously described primers and PCR procedures. Identification of MecA and PVL genes attributed to MRSA was also done with PCR.

Results

A total of 322 participants between 21 and 28 years were recruited representing 5 different faculties of study. Seventy one (22.0%) were colonized with S.aureus and 14 among them with MRSA, making the MRSA colonization rate of 4.3%. Forty five (15%) of the participants were colonized with an ESBL producing E.coli or Klebsiella spp. No one was colonized with carbapenem resistant E.coli or Klebsiella species. Of the 45 ESBL producers the commonest genetic determinant identified was blaCTX-M (n = 36), while 16 isolates had blaTEM and 7 had blaSHV. Similarly, of the 14 isolates identified as MRSA, 3 (21.4%) were found to be PVL positive while 11 (78.6%) were MecA positive.

Conclusions

A high rate of colonization with ESBL producing E.coli and Klebsiella species was noted in our study group.
Comparison of the effectiveness of lecture instruction and virtual reality-based serious gaming instruction on the medical students’ learning outcome about approach to coma
Authors: Mansoory Meysam Siyah , Khazaei Mohammad Rasool , Azizi Seyyed Mohsen , Niromand Elham
Published: June 2021

Background

New approaches to e-learning and the use of virtual reality technology and serious game in medical education are on the rise. Therefore, the purpose of this study was to compare the effectiveness of lecture method and virtual reality-based serious gaming (VRBSG) method on students learning outcomes about the approach to coma.

Methods

We adopted a randomized trial method for this study and selected 50 medical students dividing them into experimental and control groups. Students’ learning outcome was measured with a 10-item test. Serious game usability scale was used to evaluate the usability of the serious game. Descriptive and inferential statistics were used for data analysis by SPSS-22 software.

Results

Students’ familiarity with e-learning and VRBSG was low. The mean usability of a VRBSG was 126.78 ± 10.34 out of 150. The majority of students were eager to be instructed through VRBSG. The mean score of learning outcomes in the experimental group was significantly higher than the control group (t = − 2.457, P = 0.019).

Conclusion

Students’ learning outcomes in the VRBSG group in the test approach to coma were significantly better than the lecture group. The usability of the serious game instruction method was high. Taken together, instruction through VRBSG had an effective role in medical students’ learning.