bluuebunny/crossref_metadata_2025 huggingface dataset:
Single Column Model Simulations of Icing Conditions in Northern Sweden: Sensitivity to Surface Model Land Use Representation
Authors: Janzon Erik , Körnich Heiner , Arnqvist Johan , Rutgersson Anna
Published: August 2020

In-cloud ice mass accretion on wind turbines is a common challenge that is faced by energy companies operating in cold climates. On-shore wind farms in Scandinavia are often located in regions near patches of forest, the heterogeneity length scales of which are often less than the resolution of many numerical weather prediction (NWP) models. The representation of these forests—including the cloud water response to surface roughness and albedo effects that are related to them—must therefore be parameterized in NWP models used as meteorological input in ice prediction systems, resulting in an uncertainty that is poorly understood and, to the present date, not quantified. The sensitivity of ice accretion forecasts to the subgrid representation of forests is examined in this study. A single column version of the HARMONIE-AROME three-dimensional (3D) NWP model is used to determine the sensitivity of the forecast of ice accretion on wind turbines to the subgrid forest fraction. Single column simulations of a variety of icing cases at a location in northern Sweden were examined in order to investigate the impact of vegetation cover on ice accretion in varying levels of solar insolation and wind magnitudes. In mid-winter cases, the wind speed response to surface roughness was the primary driver of the vegetation effect on ice accretion. In autumn cases, the cloud water response to surface albedo effects plays a secondary role in the impact of in-cloud ice accretion, with the wind response to surface roughness remaining the primary driver for the surface vegetation impact on icing. Two different surface boundary layer (SBL) forest canopy subgrid parameterizations were tested in this study that feature different methods for calculating near-surface profiles of wind, temperature, and moisture, with the ice mass accretion again following the wind response to surface vegetation between both of these schemes.
Fault Ride through Capability Analysis (FRT) in Wind Power Plants with Doubly Fed Induction Generators for Smart Grid Technologies
Authors: Nduwamungu Aphrodis , Ntagwirumugara Etienne , Mulolani Francis , Bashir Waqar
Published: August 2020

Faults in electrical networks are among the key factors and sources of network disturbances. Control and automation strategies are among the key fault clearing techniques responsible for the safe operation of the system. Several researchers have revealed various constraints of control and automation strategies such as a slow dynamic response, the inability to switch the network on and off remotely, a high fault clearing time and loss minimization. For a system with wind energy technologies, if the power flow of a wind turbine is perturbed by a fault, the intermediate circuit voltage between the machine side converter and line side converter will rise to unacceptably high values due to the accumulation of energy in the DC link capacitor. To overcome the aforementioned issues, this paper used MATLAB simulations and experiments to analyze and validate the results. The results revealed that fault ride through capability with Supervisory Control and Data Acquisition (SCADA) viewer software, Active Servo software and wind sim packages are more adaptable to the variations of voltage sag, voltage swell and wind speed and avoid loss of synchronism and improve power quality. Furthermore, for protection purposes, a DC chopper and a crowbar should be incorporated into the management of excess energy during faults and a ferrite device included for the reduction of the electromagnetic field.
Power and Wind Shear Implications of Large Wind Turbine Scenarios in the US Central Plains
Authors: Barthelmie Rebecca J. , Shepherd Tristan J. , Aird Jeanie A. , Pryor Sara C.
Published: August 2020

Continued growth of wind turbine physical dimensions is examined in terms of the implications for wind speed, power and shear across the rotor plane. High-resolution simulations with the Weather Research and Forecasting model are used to generate statistics of wind speed profiles for scenarios of current and future wind turbines. The nine-month simulations, focused on the eastern Central Plains, show that the power scales broadly as expected with the increase in rotor diameter (D) and wind speeds at hub-height (H). Increasing wind turbine dimensions from current values (approximately H = 100 m, D = 100 m) to those of the new International Energy Agency reference wind turbine (H = 150 m, D = 240 m), the power across the rotor plane increases 7.1 times. The mean domain-wide wind shear exponent (α) decreases from 0.21 (H = 100 m, D = 100 m) to 0.19 for the largest wind turbine scenario considered (H = 168 m, D = 248 m) and the frequency of extreme positive shear (α > 0.2) declines from 48% to 38% of 10-min periods. Thus, deployment of larger wind turbines potentially yields considerable net benefits for both the wind resource and reductions in fatigue loading related to vertical shear.
Predicting Suitable Habitats of Camptotheca acuminata Considering Both Climatic and Soil Variables
Authors: Feng Lei , Sun Jiejie , Shi Yuanbao , Wang Guibin , Wang Tongli
Published: August 2020

Camptotheca acuminata is considered a natural medicinal plant with antitumor activity. The assessment of climate change impact on its suitable habitats is important for cultivation and conservation. In this study, we applied a novel approach to build ecological niche models with both climate and soil variables while the confounding effects between the variables in the two categories were avoided. We found that the degree-days below zero and mean annual precipitation were the most important climatic factors, while the basic soil saturation, soil gravel volume percentage, and clay content were the main soil factors, determining the suitable habitats of C. acuminata. We found that suitable habitats of this species would moderately increase in future climates under both the RCP4.5 and RCP8.5 climate change scenarios for the 2020s, 2050s, and 2080s. However, substantial shifts among levels of habitat suitability were projected. The dual high-suitable habitats would expand, which would be favorable for commercial plantations. Our findings contribute to a better understanding of the impact of climate change on this species and provide a scientific basis for the cultivation and conservation purposes.
Profiling Individual Differences in Alcoholic Beverage Preference and Consumption: New Insights from a Large-Scale Study
Authors: Cravero Maria Carla , Laureati Monica , Spinelli Sara , Bonello Federica , Monteleone Erminio , Proserpio Cristina , Lottero Maria Rosa , Pagliarini Ella , Dinnella Caterina
Published: August 2020

Alcoholic beverage consumption plays an important role in European culture, and in many contexts drinking alcohol is socially acceptable and considered part of the diet. Understanding the determinants of alcohol preference and consumption is important not only for disease prevention, intervention, and policy management, but also for market segmentation, product development, and optimization. The aim of the study was to investigate the effect of individual responsiveness to various oral sensations on self-reported liking and intake of 14 alcoholic and non-alcoholic beverages (including beers, wines, spirits, and cocktails) considering gender, age, and oral responsiveness (measured through response to 6-n-propyl-2-thiouracil -PROP, basic tastes, astringency, and pungency) in a large sample of Italian consumers. Data were collected from 2388 respondents (age range 18–60 years; mean age = 37.6, SD = 13.1; 58.2% women). These results indicate that notwithstanding the strong gender difference, with women generally liking and consuming fewer alcoholic beverages than men, liking patterns in the two genders were similar. Three liking patterns for different alcoholic beverages largely driven by orosensory properties were identified in both genders. “Spirit-lovers” constituted the smallest group (12%), consumed alcoholic beverages of any kind (not only spirits) more than the other segments, and were mainly men aged 30–45. “Beer/wine lovers” (44%) were the oldest group with no difference by gender. “Mild-drink lovers” (44%) liked alcoholic drinks with intense sweet taste and/or mixers that moderate ethanol perception. They were mainly women, aged 18–29, had a lower consumption of alcohol, and a higher orosensory responsiveness than the other two groups. The results also suggest the opportunity to develop personalized recommendations towards specific consumer segments based not only on socio-demographics but considering also perceptive variables. Finally, our data suggest that increased burning and bitterness from alcohol may act as a sensory hindrance to alcoholic beverage overconsumption.
The Combined Effect of Pressure and Temperature on Kefir Production—A Case Study of Food Fermentation in Unconventional Conditions
Authors: Ribeiro Ana C. , Lemos Álvaro T. , Lopes Rita P. , Mota Maria J. , Inácio Rita S. , Gomes Ana M. P. , Sousa Sérgio , Delgadillo Ivonne , Saraiva Jorge A.
Published: August 2020

Food fermentation under pressure has been studied in recent years as a way to produce foods with novel properties. The purpose of this work was to study kefir production under pressure (7–50 MPa) at different temperatures (17–32 °C), as a case study of unconventional food fermentation. The fermentation time to produce kefir was similar at all temperatures (17, 25, and 32 °C) up to 15 MPa, compared to atmospheric pressure. At 50 MPa, the fermentation rate was slower, but the difference was reduced as temperature increased. During fermentation, lactic and acetic acid concentration increased while citric acid decreased. The positive activation volumes (Va) obtained indicate that pressure decreased the fermentation rate, while the temperature rise led to the attenuation of the pressure effect (lower Va). On the other hand, higher activation energies (Ea) were observed with pressure increase, indicating that fermentation became more sensitive to temperature. The condition that resulted in a faster fermentation, higher titratable acidity, and higher concentration of lactic acid was 15 MPa/32 °C. As the authors are aware, this is the second work in the literature to study the combined effect of pressure and temperature on a fermentative process.
Dispersive Transport Described by the Generalized Fick Law with Different Fractional Operators
Authors: Sibatov Renat T. , Sun HongGuang
Published: August 2020

The approach based on fractional advection–diffusion equations provides an effective and meaningful tool to describe the dispersive transport of charge carriers in disordered semiconductors. A fractional generalization of Fick’s law containing the Riemann–Liouville fractional derivative is related to the well-known fractional Fokker–Planck equation, and it is consistent with the universal characteristics of dispersive transport observed in the time-of-flight experiment (ToF). In the present paper, we consider the generalized Fick laws containing other forms of fractional time operators with singular and non-singular kernels and find out features of ToF transient currents that can indicate the presence of such fractional dynamics. Solutions of the corresponding fractional Fokker–Planck equations are expressed through solutions of integer-order equation in terms of an integral with the subordinating function. This representation is used to calculate the ToF transient current curves. The physical reasons leading to the considered fractional generalizations are elucidated and discussed.
Autohistoria: Traversing through Time and Space to Explore Identity, Consciousness, Positionality, and Power
Authors: Flores Belinda Bustos
Published: August 2020

How do our own cultural-historical experiences in geographic spaces like the border(s) we occupy shape our identities, consciousness, positionality, and power? Using the autohistoria-teoria methodology, the intent of this manuscript is to explore my paternal grandmother’s family, Los Martínez’ cultural historical experiences as descendants of conquistadores, who eventually lived along the Rio Grande-Río Bravo del Norte, which is now the Texas–Mexico border. Archival data, including birth, marriage, and death certificates, land grants, maps, border crossing documents, published books, and family oral stories were used to establish a timeline and develop a narrative that spans across time and geographic zones that were originally indigenous, colonized by Spain, became México, and for some of these territories eventually became part of the United States. I will share Los Martínez’ origins that begin in the Kingdom of the Navarre, their story as conquistadores and settlers in northern México and Texas geographic areas that were part of Nuevo España. The overarching theme I plan to capture is the fluidity of borders as figured worlds, but I also plan to highlight the formation of hybrid identities, consciousness, positionality, and power within the spaces/figured worlds that we occupy as both colonizer and colonized.
Integrated Morpho-Bathymetric, Seismic-Stratigraphic, and Sedimentological Data on the Dohrn Canyon (Naples Bay, Southern Tyrrhenian Sea): Relationships with Volcanism and Tectonics
Authors: Aiello Gemma , Iorio Marina , Molisso Flavia , Sacchi Marco
Published: August 2020

Submarine canyons are geomorphologic lineaments engraving the slope/outer shelf of continental margins. These features are often associated with significant geologic hazard when they develop close to densely populated coastal zones. The seafloor of Naples Bay is deeply cut by two incisions characterized by a dense network of gullies, namely the Dohrn and Magnaghi canyons, which develop from the shelf break of the Campania margin, down to the peripheral rise of the Eastern Tyrrhenian bathyal plain. Seismic-stratigraphic interpretation of multichannel seismic reflection profiles has shown that quaternary tectonics and recent to active volcanism have exerted a significant control on the morphological evolution and source-to sink depositional processes of the Dohrn and Magnaghi submarine canyons. The Dohrn canyon is characterized by relatively steep walls hundreds of meters high, which incise a Middle-Late Pleistocene prograding wedge, formed by clastic and volcaniclastic deposits associated with the paleo-Sarno river system during the Mid-Late Pleistocene. The formation of the Dohrn canyon predates the onset of the volcanic eruption of the Neapolitan Yellow Tuff (NYT), an ignimbrite deposit of ca. 15 ka that represents the bedrock on which the town of Napoli is built. Integrated stratigraphic analysis of high-resolution seismic profiles and marine gravity core data (C74_12) collected along the flanks of the eastern bifurcation of the head of Dohrn Canyon suggests that depositional processes along the canyon flanks are dominated by gravity flows (e.g., fine-grained turbidites, debris flows) and sediment mass transport associated with slope instability and failure.
Open-Source MASW Inversion Tool Aimed at Shear Wave Velocity Profiling for Soil Site Explorations
Authors: Olafsdottir Elin Asta , Erlingsson Sigurdur , Bessason Bjarni
Published: August 2020

The shear wave velocity profile is of primary interest for geological characterization of soil sites and elucidation of near-surface structures. Multichannel Analysis of Surface Waves (MASW) is a seismic exploration method for determination of near-surface shear wave velocity profiles by analyzing Rayleigh wave propagation over a wide range of wavelengths. The inverse problem faced during the application of MASW involves finding one or more layered soil models whose theoretical dispersion curves match the observed dispersion characteristics. A set of open-source MATLAB-based tools for acquiring and analyzing MASW field data, MASWaves, has been under development in recent years. In this paper, a new tool, using an efficient Monte Carlo search technique, is introduced to conduct the inversion analysis in order to provide the shear wave velocity profile. The performance and applicability of the inversion scheme is demonstrated with synthetic datasets and field data acquired at a well-characterized geotechnical research site.
Living Suspiciously: Contingent Belonging in British South Asian Theater
Authors: de Waal Ariane
Published: August 2020

This article investigates representations of national belonging in British South Asian theater productions after the 2005 London bombings. It identifies a significant yet hitherto underresearched corpus of plays that show the formation of the UK “home front” in the war on terror from the perspective of postcolonial subjects who are deemed threatening rather than worthy of protection. After discussing the construction of British South Asian citizens as suspicious subjects, the article analyzes two plays that offer an extensive consideration of the contingencies of national belonging. It argues that True Brits by Vinay Patel and Harlesden High Street by Abhishek Majumdar dramatize strategies for building, making, or keeping a home in London in spite of the strictures of suspectification and securitization.
Evaluating the Facilitators, Barriers, and Medical Outcomes Commensurate with the Use of Assistive Technology to Support People with Dementia: A Systematic Review Literature
Authors: Kruse Clemens Scott , Fohn Joanna , Umunnakwe Gilson , Patel Krupa , Patel Saloni
Published: August 2020

Background: Assistive technologies (AT) have been used to improve the daily living conditions of people living with dementia (PWD). Research supports the positive impact of the use of AT such as decreased burden on caregivers and behavioral support for people with dementia. Four reviews in the last six years have analyzed AT and PWD, but none have incorporated the dimension of medical outcomes. Objectives: The purpose of this review is to identify the facilitators, barriers, and medical outcomes commensurate with the use of AT with PWD. Method: This review queried The Cumulative Index of Nursing and Allied Health Literature (CINAHL), Web of Science, Science Direct, and PubMed databases for peer-reviewed publications in the last five years for facilitators, barriers, and medical outcomes commensurate with the use of AT with PWD. The study is reported and conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) and the Kruse Protocol for conducting a systematic review. Results: 48 studies were analyzed. Fourteen types of AT, 17 facilitators, 17 barriers, and 16 medical outcomes were identified in the literature. The two most frequently mentioned ATs were cognitive stimulators (9/48, 19%) and social robots (5/48, 10%). The two most frequently mentioned facilitators were caregivers want AT (8/68, 12%) and enables increased independence (7/68, 10%). The top two barriers were cost (8/75, 11%) and PWD reject AT (8/75, 11%). The top medical outcomes were improved cognitive abilities (6/69, 9%), increased activities of daily living (ADLs), and increased autonomy (each at 5/69, 7%): Zero negative outcomes were reported. Conclusion: The systematic review revealed the positive relations that occur when PWD and their caregivers use AT. Although many reservations surrounding the use of AT exist, a majority of the literature shows a positive effect of its use. Research supports a strong support for AT by caregivers due to many positive medical outcomes, but also a reticence to adopt by PWD. If ATs for PWD are a way to reduce stress on caregivers, barriers of cost and complexity need to be addressed through health policy or grants.
Investigation of the Pigments and Glassy Matrix of Painted Enamelled Qing Dynasty Chinese Porcelains by Noninvasive On-Site Raman Microspectrometry
Authors: Colomban Philippe , Kırmızı Burcu , Zhao Bing , Clais Jean-Baptiste , Yang Yong , Droguet Vincent
Published: August 2020

A selection of 15 Chinese painted enameled porcelains from the 18th century (Qing dynasty) was analyzed on-site by mobile Raman and XRF microspectroscopy. The highly prized artifacts are present in the collections of the Musée du Louvre in Paris and Musée Chinois at Fontainebleau Castle in France. In the painted enamels, pigments such as Naples yellow lead pyrochlore, hematite, manganese oxide and carbon and opacifiers such as lead arsenates were detected. The glassy matrices of the enamels mainly belonged to lead-rich and lead-alkali glass types according to the Raman spectra obtained. The glaze and body phases of the porcelain artifacts were also analyzed. The detection of lead arsenate apatite in some of the blue enamels was significant, indicating the use of arsenic-rich European cobalt ores (smalt) and possibly mixing with Asian cobalt. This characteristic phase has also been identified in French soft-paste porcelains and glass decor and high-quality Limoges enamels from the same period. Based on the shape of the Raman scattering background, the presence of colloidal gold (Au° nanoparticles) was identified in red, orange and pink enamels. Different types of Naples yellow pigments were also detected with Sb-rich, Sn-rich and mixed Sb–Sn–(Zn, Fe?) compositions in the yellow enamels. The results were compared to previous data obtained on Chinese cloisonné and painted enameled metalware and Limoges enamels as well as French enameled watches.
Long-Term Groundwater Level Prediction Model Based on Hybrid KNN-RF Technique
Authors: Kombo Omar , Kumaran Santhi , Sheikh Yahya , Bovim Alastair , Jayavel Kayalvizhi
Published: August 2020

Reliable seasonal prediction of groundwater levels is not always possible when the quality and the amount of available on-site groundwater data are limited. In the present work, a hybrid K-Nearest Neighbor-Random Forest (KNN-RF) is used for the prediction of variations in groundwater levels (L) of an aquifer with the groundwater relatively close to the surface (<10 m) is proposed. First, the time-series smoothing methods are applied to improve the quality of groundwater data. Then, the ensemble K-Nearest Neighbor-Random Forest (KNN-RF) model is treated using hydro-climatic data for the prediction of variations in the levels of the groundwater tables up to three months ahead. Climatic and groundwater data collected from eastern Rwanda were used for validation of the model on a rolling window basis. Potential predictors were: the observed daily mean temperature (T), precipitation (P), and daily maximum solar radiation (S). Previous day’s precipitation P (t − 1), solar radiation S (t), temperature T (t), and groundwater level L (t) showed the highest variation in the fluctuations of the groundwater tables. The KNN-RF model presents its results in an intelligible manner. Experimental results have confirmed the high performance of the proposed model in terms of root mean square error (RMSE), mean absolute error (MAE), Nash–Sutcliffe (NSE), and coefficient of determination (R2).
Understanding Aging Policies in China: A Bibliometric Analysis of Policy Documents, 1978–2019
Authors: Nan Yan , Feng Tieying , Hu Yuqun , Qi Xinzhu
Published: August 2020

Aging poses a big challenge in all aspects of social governance in China. A coherent and focused aging policy response that spans multiple sectors of government has been undertaken to achieve the goal of “Healthy Aging”. From an historical perspective, this paper uses a bibliometric analysis method to probe into the evolution of Chinese aging policies from 1978 to 2019, and the roles of core government agencies in policy-making. We obtained 226 Chinese aging policies from the PKULaw Database and the websites of the government departments. Co-word analyses and network analyses were applied in mapping the topics of aging policies and collaboration among the agencies. Gephi software was used to visualize the most frequently used keywords and their network graphs. Findings are as follows. Firstly, the development of the aging policy system in China has undergone two phases, from focusing on basic security to emphasizing the rights and health of the elderly. Secondly, the network structure of aging policy-making departments presents a distinct edge-core layer. More and more government agencies have become involved in the formulation of aging policies. But collaboration among the agencies is insufficient. Thirdly, pilot promotion is the main tool for implementing aging policies.
A Carbon Emission Measurement Method for Individual Travel Based on Transportation Big Data: The Case of Nanjing Metro
Authors: Yu Wei , Wang Tao , Xiao Yujie , Chen Jun , Yan Xingchen
Published: August 2020

With the strengthening of environmental awareness, the government pays much more attention to environmental protection and thus implements carbon trading schemes to promote the reduction of global carbon dioxide emissions. The carbon Generalized System of Preferences (GSP) is an incentive mechanism for citizens to value their energy conservation and carbon reduction. Individual travel needs to rely on various means of transportation, resulting in energy consumption. Carbon tax or subsidy can only be carried out after carbon GSP accurately measures individual carbon emissions. The big data acquired from the smart cards of passengers’ travels provide the possibility for carbon emission accounting of individual travel. This research proposes a carbon emission measurement of individual travel. Through establishing the network model of the Nanjing metro with a complex method, the shortest path of the passengers’ travels is obtained. Combined with the origination–destination (OD) records of the smart cards, the total distance of the passengers’ travels is obtained. By selecting the operation table to estimate the carbon emissions generated by the daily operation of the subway system, the carbon emissions per kilometer or per time of passenger travel are finally obtained. With the accurate tracking of carbon emissions for individual travel, the government may establish a comprehensive monitoring system so as to establish a carbon tax and carbon supplement mechanism for citizens.
Estimating Disability-Adjusted Life Years due to Tuberculosis in Korea through to the Year 2040
Authors: Jang Su Yeon , Kim Moon Jung , Cheong Hyeon-Kyoung , Oh In-Hwan
Published: August 2020

Despite efforts to reduce its incidence, tuberculosis continues to burden the rapidly aging Korean society. This study aimed to investigate the current trend of tuberculosis burden in Korea and its projections to 2040. We used National Health Insurance claims data to calculate the disability-adjusted life years due to tuberculosis in Korea. Disability-adjusted life years were measured by summing the years of life lost and the years lived with disability using an incidence-based approach. We modeled the incidence rates using a time-series model for the projection of disability-adjusted life years accrued from 2020 to 2040. The total disability-adjusted life years due to tuberculosis were 69, 64, 59, and 49 disability-adjusted life years/100,000 population in 2014, 2015, 2016, and 2017, respectively. In both sexes, disability-adjusted life years were the highest in those aged ≥80 years. Projected disability-adjusted life years showed a descending trend from 38 disability-adjusted life years/100,000 in 2020, to 14 disability-adjusted life years/100,000 in 2040. Conversely, the projected disability-adjusted life years increased among females aged ≥80 years. Although the tuberculosis burden in Korea is decreasing, a high burden remains among the elderly. Therefore, interventions targeting those vulnerable are required.
Community Pharmacist’s Role in Detecting Low Back Pain, and Patient Attitudes—A Cross-Sectional Observational Study in Italian Community Pharmacies
Authors: Giua Corrado , Minghetti Paola , Gandolini Giorgio , Rocco Paolo , Arancio Elisa , Bevacqua Teresa , Floris Nicolina , Keber Enrico , SGCP  , Musazzi Umberto M.
Published: August 2020

Background: Low back pain (LBP) is one of the most frequent diseases for which patients seek advice in a community pharmacy. The study aimed to evaluate the feasibility of the administration by community pharmacists of questionnaires to assess the LBP intensity and disability degree in patients entering community pharmacies and the attitudes they have toward pain management by pharmacological and non-pharmacological strategies. Methods: An explorative, cross-sectional, observational, and quantitative study was performed. Twelve Italian community pharmacists were asked to submit a questionnaire on LBP to patients visiting their pharmacies. The questionnaire included a pain intensity scale, and two validated tools: the Roland and Morris Disability Questionnaire (RMDQ) and the Start Back Screening Tool (SBST) to determine the degree and risk of patient disability, respectively. Results: 872 patients filled out the questionnaires in 6 months. No statistical differences between genders (p > 0.30) were recorded for pain intensity (Female: median score 6, IQR 4–7; Male: median scores 5, IQR 4–7; p > 0.30) and disability associated with LBP (RMDQ high-disability level: Females, 14.7%, Males, 15.0%; p > 0.90). Most of the patients (69%) reported a low degree of disability, but the risk of disability was medium and high in 36% and 18% of them, respectively (p < 0.05). About 14% of patients declare to never seek for physician’s advice despite their medium-high degree of disability. Conclusion: The study demonstrated the feasibility of validated tools for assessing the degree and risk of disability in LBP patients administrable in community pharmacies. Moreover, the community pharmacy resulted in an important care portal for patients suffering from moderate LBP and for intercepting patients who suffered from severe LBP but had never reported their problem to their physician.
Indicators of Absolute and Relative Changes in Skeletal Muscle Mass during Adulthood and Ageing
Authors: Dopsaj Milivoj , Kukić Filip , Đorđević-Nikić Marina , Koropanovski Nenad , Radovanović Dragan , Miljuš Dragan , Subošić Dane , Tomanić Milena , Dopsaj Violeta
Published: August 2020

This study aimed to explore the set of variables related to skeletal muscle mass (SMM) in both sexes, and to create age- and sex-related models of changes in SMM, using the most representative indicator of muscular status. Body composition was assessed in 8733 subjects (♀ = 3370 and ♂ = 5363), allocated into subsamples according to age: 18–29.9, 30–39.9, 40–49.9, 50–59.9, 60–69.9, and 70.0–79.9 years. Nine variables were used: protein mass, protein percent, protein mass index, SMM, percent of SMM, SMM index, fat-free mass, fat-free mass index, and protein/fat index. Univariate and multivariate analysis of variance (ANOVA and MANOVA) were used to determine between- and within-sex difference in all variables by age. Correlation analysis established the relationship between age and muscularity variables. Principal Component Analysis extracted the variables that loaded highest in explaining muscularity, while regression analysis determined the linearity of association between the age and indicators of muscular status. Variables SMMI and PSMM were extracted as the most sensitive to age, with SMMI being gender-independent while showing the parabolic and sinusoidal form of change as function of ageing in males and females, respectively; and PSMM being sex-dependent while showing a linear trend of decrease in both sexes.
Narrative Review on Health-EDRM Primary Prevention Measures for Vector-Borne Diseases
Authors: Chan Emily , Sham Tiffany , Shahzada Tayyab , Dubois Caroline , Huang Zhe , Liu Sida , Hung Kevin , Tse Shelly , Kwok Kin , Chung Pui-Hong , Kayano Ryoma , Shaw Rajib
Published: August 2020

Climate change is expanding the global at-risk population for vector-borne diseases (VBDs). The World Health Organization (WHO) health emergency and disaster risk management (health-EDRM) framework emphasises the importance of primary prevention of biological hazards and its value in protecting against VBDs. The framework encourages stakeholder coordination and information sharing, though there is still a need to reinforce prevention and recovery within disaster management. This keyword-search based narrative literature review searched databases PubMed, Google Scholar, Embase and Medline between January 2000 and May 2020, and identified 134 publications. In total, 10 health-EDRM primary prevention measures are summarised at three levels (personal, environmental and household). Enabling factor, limiting factors, co-benefits and strength of evidence were identified. Current studies on primary prevention measures for VBDs focus on health risk-reduction, with minimal evaluation of actual disease reduction. Although prevention against mosquito-borne diseases, notably malaria, has been well-studied, research on other vectors and VBDs remains limited. Other gaps included the limited evidence pertaining to prevention in resource-poor settings and the efficacy of alternatives, discrepancies amongst agencies’ recommendations, and limited studies on the impact of technological advancements and habitat change on VBD prevalence. Health-EDRM primary prevention measures for VBDs require high-priority research to facilitate multifaceted, multi-sectoral, coordinated responses that will enable effective risk mitigation.
The Impact of a Multi-Pronged Intervention on Students’ Perceptions of School Lunch Quality and Convenience and Self-Reported Fruit and Vegetable Consumption
Authors: Machado Stephanie S. , Ritchie Lorrene D. , Thompson Hannah R. , Madsen Kristine A.
Published: August 2020

School lunch programs provide an opportunity to improve students’ diets. We sought to determine the impact of a multifaceted intervention (cafeteria redesigns, increased points-of-sale and teacher education) on secondary students’ perceptions of school-lunch quality and convenience and fruit and vegetable intake. Surveys (n = 12,827) from middle and high school students in 12 intervention and 11 control schools were analyzed. We investigated change in school-lunch perceptions and lunchtime and daily fruit and vegetable consumption from 2016 to 2018. Among 8th graders, perceptions that school lunch tastes good and that school lunch was enough to make students feel full increased 0.2 points (on a 5-point scale; p < 0.01) in intervention schools relative to control schools. Among 10th graders, lunchtime fruit and vegetable consumption increased 6% in intervention relative to control schools (p < 0.05 and p < 0.01 respectively). Daily fruit intake increased 0.1 cups/day in intervention relative to control schools among 9th graders (p < 0.01). This study provides important evidence on the limited effect of design approaches in the absence of meal changes. We observed only modest changes in school lunch perceptions and fruit and vegetable consumption that were not consistent across grades, suggesting that additional efforts are needed to improve school-lunch uptake.
High-Flow Oxygen through Nasal Cannula vs. Non-Invasive Ventilation in Hypercapnic Respiratory Failure: A Randomized Clinical Trial
Authors: Papachatzakis Yiannis , Nikolaidis Pantelis Theodoros , Kontogiannis Sofoklis , Trakada Georgia
Published: August 2020

High-flow oxygen through nasal cannula (HFNC) provides adequate oxygenation and can be an alternative to noninvasive ventilation (NIV) for patients with hypoxemic respiratory failure. The aim of the present study was to assess the efficacy of HFNC versus NIV in hypercapnic respiratory failure. Patients (n = 40) who were admitted to the Emergency Department of Alexandra Hospital due to hypercapnic respiratory failure (PaCO2 ≥ 45 mmHg) were randomized assigned into two groups, i.e., an intervention group (use of HFNC, n = 20) and a control group (use of NIV, n = 20). During their hospitalization in the Intensive Care Unit, vital signs (respiratory and heart rate, arterial blood pressure) and arterial blood gases (ABG) were closely monitored on admission, after 24 h and at discharge. No difference between the two groups regarding the duration of hospitalization and the use of HFNC or NIV was observed (p > 0.05). On admission, the two groups did not differ in terms of gender, age, body mass index, APACHE score, predicted death rate, heart rate, arterial blood pressure and arterial blood gases (p > 0.05). Respiratory rate in the HFNC group was lower than in the NIV group (p = 0.023). At discharge, partial carbon dioxide arterial pressure (PaCO2) in the HFNC group was lower than in the NIV group (50.8 ± 9.4 mmHg versus 59.6 ± 13.9 mmHg, p = 0.024). The lowerPaCO2 in the HFNC group than in the NIV group indicated that HFNC was superior to NIV in the management of hypercapnic respiratory failure.
Affinity Capture Enrichment versus Affinity Depletion: A Comparison of Strategies for Increasing Coverage of Low-Abundant Human Plasma Proteins
Authors: Palstrøm Nicolai Bjødstrup , Rasmussen Lars Melholt , Beck Hans Christian
Published: August 2020

In the present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from plasma samples thereby enabling the detection of medium-to-low abundant proteins in plasma samples by mass spectrometry-based proteomics. We compared their performance with the most commonly used immunodepletion method, the Multi Affinity Removal System Human 14 (MARS14) targeting the top 14 most abundant plasma proteins and also the ProteoMiner protein equalization method by label-free quantitative liquid chromatography tandem mass spectrometry (LC-MSMS) analysis. The affinity-based probes demonstrated a high reproducibility for low-abundant plasma proteins, down to picomol per mL levels, compared to the Multi Affinity Removal System (MARS) 14 and the Proteominer methods, and also demonstrated superior removal of the majority of the high-abundant plasma proteins. The ABA-based affinity probe and the Proteominer protein equalization method performed better compared to all other methods in terms of the number of analyzed proteins. All the tested methods were highly reproducible for both high-abundant plasma proteins and low-abundant proteins as measured by correlation analyses of six replicate experiments. In conclusion, our results demonstrated that small-molecule based affinity-based probes are excellent alternatives to the commonly used immune-depletion methods for proteomic biomarker discovery studies in plasma. Data are available via ProteomeXchange with identifier PXD020727.
Transcriptome Analysis Elucidates the Key Responses of Bryozoan Fredericella sultana during the Development of Tetracapsuloides bryosalmonae (Myxozoa)
Authors: Kumar Gokhlesh , Ertl Reinhard , Bartholomew Jerri L. , El-Matbouli Mansour
Published: August 2020

Bryozoans are sessile, filter-feeding, and colony-building invertebrate organisms. Fredericella sultana is a well known primary host of the myxozoan parasite Tetracapsuloides bryosalmonae. There have been no attempts to identify the cellular responses induced in F. sultana during the T. bryosalmonae development. We therefore performed transcriptome analysis with the aim of identifying candidate genes and biological pathways of F. sultana involved in the response to T. bryosalmonae. A total of 1166 differentially up- and downregulated genes were identified in the infected F. sultana. Gene ontology of biological processes of upregulated genes pointed to the involvement of the innate immune response, establishment of protein localization, and ribosome biogenesis, while the downregulated genes were involved in mitotic spindle assembly, viral entry into the host cell, and response to nitric oxide. Eukaryotic Initiation Factor 2 signaling was identified as a top canonical pathway and MYCN as a top upstream regulator in the differentially expressed genes. Our study provides the first transcriptional profiling data on the F. sultana zooid’s response to T. bryosalmonae. Pathways and upstream regulators help us to understand the complex interplay in the infected F. sultana. The results will facilitate the elucidation of innate immune mechanisms of bryozoan and will lay a foundation for further analyses on bryozoan-responsive candidate genes, which will be an important resource for the comparative analysis of gene expression in bryozoans.
R-Ras GTPases Signaling Role in Myelin Neurodegenerative Diseases
Authors: Alcover-Sanchez Berta , Garcia-Martin Gonzalo , Wandosell Francisco , Cubelos Beatriz
Published: August 2020

Myelination is required for fast and efficient synaptic transmission in vertebrates. In the central nervous system, oligodendrocytes are responsible for creating myelin sheaths that isolate and protect axons, even throughout adulthood. However, when myelin is lost, the failure of remyelination mechanisms can cause neurodegenerative myelin-associated pathologies. From oligodendrocyte progenitor cells to mature myelinating oligodendrocytes, myelination is a highly complex process that involves many elements of cellular signaling, yet many of the mechanisms that coordinate it, remain unknown. In this review, we will focus on the three major pathways involved in myelination (PI3K/Akt/mTOR, ERK1/2-MAPK, and Wnt/β-catenin) and recent advances describing the crosstalk elements which help to regulate them. In addition, we will review the tight relation between Ras GTPases and myelination processes and discuss its potential as novel elements of crosstalk between the pathways. A better understanding of the crosstalk elements orchestrating myelination mechanisms is essential to identify new potential targets to mitigate neurodegeneration.
Basic and Translational Models of Cooperative Oncogenesis
Authors: Richardson Helena E. , Cordero Julia B. , Grifoni Daniela
Published: August 2020

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CD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood
Authors: Krijgsman Daniëlle , De Vries Natasja L. , Andersen Morten N. , Skovbo Anni , Tollenaar Rob A.E.M. , Møller Holger J. , Hokland Marianne , Kuppen Peter J.K.
Published: August 2020

The macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to clinicopathological parameters. An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum sCD163 levels and multiparameter flow cytometry was used to study the peripheral blood monocytes and their CD163 expression in CRC patients (N = 78) and healthy donors (N = 50). The distribution of tumor-associated macrophages (TAMs) was studied in primary colorectal tumors with multiplex immunofluorescence. We showed that CRC patients with above-median sCD163 level had a shorter overall survival (OS, p = 0.035) as well as disease-free survival (DFS, p = 0.005). The above-median sCD163 remained significantly associated with a shorter DFS in the multivariate analysis (p = 0.049). Moreover, a shorter OS was observed in CRC patients with an above-median total monocyte percentage (p = 0.007). The number and phenotype of the stromal and intraepithelial TAMs in colorectal tumors were not associated with clinical outcome. In conclusion, sCD163 and monocytes in the circulation may be potential prognostic biomarkers in CRC patients, whereas TAMs in the tumor showed no association with clinical outcome. Thus, our results emphasize the importance of the innate systemic immune response in CRC disease progression.
The Significance of Flavonoids in the Process of Biological Nitrogen Fixation
Authors: Dong Wei , Song Yuguang
Published: August 2020

Nitrogen is essential for the growth of plants. The ability of some plant species to obtain all or part of their requirement for nitrogen by interacting with microbial symbionts has conferred a major competitive advantage over those plants unable to do so. The function of certain flavonoids (a group of secondary metabolites produced by the plant phenylpropanoid pathway) within the process of biological nitrogen fixation carried out by Rhizobium spp. has been thoroughly researched. However, their significance to biological nitrogen fixation carried out during the actinorhizal and arbuscular mycorrhiza–Rhizobium–legume interaction remains unclear. This review catalogs and contextualizes the role of flavonoids in the three major types of root endosymbiosis responsible for biological nitrogen fixation. The importance of gaining an understanding of the molecular basis of endosymbiosis signaling, as well as the potential of and challenges facing modifying flavonoids either quantitatively and/or qualitatively are discussed, along with proposed strategies for both optimizing the process of nodulation and widening the plant species base, which can support nodulation.
Tracking Antimicrobial Resistance Determinants in Diarrheal Pathogens: A Cross-Institutional Pilot Study
Authors: Taitt Chris R. , Leski Tomasz A. , Prouty Michael G. , Ford Gavin W. , Heang Vireak , House Brent L. , Levin Samuel Y. , Curry Jennifer A. , Mansour Adel , Mohammady Hanan El , Wasfy Momtaz , Tilley Drake Hamilton , Gregory Michael J. , Kasper Matthew R. , Regeimbal James , Rios Paul , Pimentel Guillermo , Danboise Brook A. , Hulseberg Christine E. , Odundo Elizabeth A. , Ombogo Abigael N. , Cheruiyot Erick K. , Philip Cliff O. , Vora Gary J.
Published: August 2020

Infectious diarrhea affects over four billion individuals annually and causes over a million deaths each year. Though not typically prescribed for treatment of uncomplicated diarrheal disease, antimicrobials serve as a critical part of the armamentarium used to treat severe or persistent cases. Due to widespread over- and misuse of antimicrobials, there has been an alarming increase in global resistance, for which a standardized methodology for geographic surveillance would be highly beneficial. To demonstrate that a standardized methodology could be used to provide molecular surveillance of antimicrobial resistance (AMR) genes, we initiated a pilot study to test 130 diarrheal pathogens (Campylobacter spp., Escherichia coli, Salmonella, and Shigella spp.) from the USA, Peru, Egypt, Cambodia, and Kenya for the presence/absence of over 200 AMR determinants. We detected a total of 55 different determinants conferring resistance to ten different categories of antimicrobials: genes detected in ≥ 25 samples included blaTEM, tet(A), tet(B), mac(A), mac(B), aadA1/A2, strA, strB, sul1, sul2, qacEΔ1, cmr, and dfrA1. The number of determinants per strain ranged from none (several Campylobacter spp. strains) to sixteen, with isolates from Egypt harboring a wider variety and greater number of genes per isolate than other sites. Two samples harbored carbapenemase genes, blaOXA-48 or blaNDM. Genes conferring resistance to azithromycin (ere(A), mph(A)/mph(K), erm(B)), a first-line therapeutic for severe diarrhea, were detected in over 10% of all Enterobacteriaceae tested: these included >25% of the Enterobacteriaceae from Egypt and Kenya. Forty-six percent of the Egyptian Enterobacteriaceae harbored genes encoding CTX-M-1 or CTX-M-9 families of extended-spectrum β-lactamases. Overall, the data provide cross-comparable resistome information to establish regional trends in support of international surveillance activities and potentially guide geospatially informed medical care.