bluuebunny/crossref_metadata_2025 huggingface dataset:
“Bitch I Said Hi”: The Bye Felipe Campaign and Discursive Activism in Mobile Dating Apps
Authors: Shaw Frances
Published: October 2016

This article examines the Instagram page for Bye Felipe, a feminist campaign where people submit screenshots of examples of harassment and sexual entitlement from men on online dating sites such as OKCupid and apps such as Tinder. I frame the campaign as an example of feminist discursive activism. The site owners collect contributions and aggregate examples of particular discursive patterns in hook up apps, in order to make collective political claims, a strategy that Tomlinson calls “intensification.” I address the existing literature on cyber-misogyny and online harassment, and also research on previous similar campaigns such as Fedoras of OKCupid to discuss shaming as a political practice. I then draw out the patterns and concepts invoked in interventions and resultant discussions on Bye Felipe, examining the themes of rejection, silence and who has the right to silence, rape culture, and gendered sexual entitlement. I identify the political claims being made through the rhetorical strategies described in the first part of the article. Drawing on the work of McCosker on trolling as provocation, I discuss the role of repetition and rehearsal in the practice of discursive politics. Finally, through a discursive analysis of responses to the posts on Instagram and Facebook over time, I explore the ongoing and difficult boundary work around what constitutes appropriate examples for the site, and the articulation of feminist claims and discourses.
Depression in Registered Nurses
Authors: Brandford Arica A. , Reed Deborah B.
Published: October 2016

Registered nurses suffer from depression at almost twice the rate of individuals in other professions. Major depression affects both employees and organizations. Depression is linked to increases in work absenteeism, short-term disability, and decreased productivity and presenteeism. Depression experienced by registered nurses may not be fully preventable, but realizing its presence and prevalence in the workplace is of vital importance. It is essential that we have a healthy, vibrant, and present registered nurse workforce to ensure that quality patient outcomes are achieved. This review of 36 articles assesses the current state of the science related to depression in registered nurses so that occupational health nurses can create pathways and work environments for better mental health for registered nurses.
Shoulder Pain Assessment for the Occupational Health Nurse
Authors: Kooienga Sarah A. , Rasmor Melody
Published: October 2016

Shoulder pain is a common complaint in the workplace. A shoulder condition may arise from acute trauma or non-traumatic work-related activities. Shoulder pain falls into three categories: acute, chronic, and referred pain. The occupational health nurse can document detailed health histories and focused complaint-driven physical examinations. Nurses’ expert assessments can guide injured workers to necessary treatment, case management, and return to full employment.
Navigating Without a Roadmap
Authors: Zubatsky Max , Aragon-Prada Mayra , Muse Fatima , Rainey Peter , Martin Robert
Published: October 2016

Caregivers of individuals with Alzheimer’s disease are willing to make large sacrifices to attend to the everyday demands of their loved one’s health. Many still lack the necessary skills, information, and resources from medical professionals needed to fully execute this role. The aim of this study is to explore the challenges that first-time Alzheimer’s caregivers encountered with members of their health care team around a loved one’s diagnosis. Four focus groups ( N = 13) were conducted in a primary care clinic and academic setting, using a semi-structured interview format facilitated by a mental health professional. The authors used thematic analysis to generate three overarching themes regarding lack of information given about the diagnosis, complications with medication management, and level of confidence in their medical team. Medical professionals should consider educating Alzheimer’s disease caregivers during the initial stage of this role, providing greater continuity of care with the family throughout treatment.
Our Essential and Endangered Dentist–Scientist Workforce
Authors: D’Souza R.N. , Colombo J.S. , Embree M.C. , Myers J.M. , DeRouen T.A.
Published: October 2016

Future advances in dental medicine rely on a robust and stable pipeline of dentist–scientists who are dedicated to research inspired by the patients’ condition. The biomedical research community faces external and internal pressures that have been building over years. This is now threatening the current and future status of basic, translational and patient-oriented research by dentist–scientists who study dental, oral and craniofacial diseases, population sciences, and prevention. The dental academic, research and practicing communities can no longer ignore the warning signs of a system that is under considerable stress. Here, the authors report findings of the Physician–Scientist Workforce Working Group, charged by the National Institutes of Health (NIH) Director, to perform quantitative and qualitative analyses on dentist–scientists by addressing the size, composition and activities of the group, relative to other health professions. From 1999 to 2012, trends in the numbers of grant applications and awards to dentist–scientists point to an overall decline. Disturbing are the low numbers of new investigators who apply for Early Career NIH Programs. While more seasoned dentist researchers enjoy greater success, the average age of first-time funded dentists is 52.7 y for females and 54.6 y for males, with a relatively low number of applications submitted and funded. These new data led the panel to stress the need to expand the capacity of the dentist–scientist workforce to leverage technologies and research opportunities that benefit the profession at-large. Suggestions were made to invest in developing clinical research faculty, including those with foreign degrees, through new training mechanisms. The creation of new alliances between national organizations like the American Association for Dental Research, the American Dental Education Association and the American Dental Association will undoubtedly lead to bold and concerted actions that must be pursued with a sense of urgency. A more supportive culture within dental schools and universities for dentist–scientists is needed, as their success is critical to the future career choices of their mentees.

Knowledge Transfer Statement: Advances in dental medicine rely on a pipeline of dentist–scientists who are dedicated to research inspired by the patients’ condition. Despite the recent advancement in technology and innovation, the dental community can no longer ignore the various pressures that threaten the future of the dentist–scientist profession. Here, the authors report findings of the Physician-Scientist Workforce Working Group of NIH that were published in 2014, and draw attention to the key issues threatening the NIH-funded pool of dentist–scientists.
Data-Driven Markov Decision Process Approximations for Personalized Hypertension Treatment Planning
Authors: Schell Greggory J. , Marrero Wesley J. , Lavieri Mariel S. , Sussman Jeremy B. , Hayward Rodney A.
Published: October 2016

Background: Markov decision process (MDP) models are powerful tools. They enable the derivation of optimal treatment policies but may incur long computational times and generate decision rules that are challenging to interpret by physicians. Methods: In an effort to improve usability and interpretability, we examined whether Poisson regression can approximate optimal hypertension treatment policies derived by an MDP for maximizing a patient’s expected discounted quality-adjusted life years. Results: We found that our Poisson approximation to the optimal treatment policy matched the optimal policy in 99% of cases. This high accuracy translates to nearly identical health outcomes for patients. Furthermore, the Poisson approximation results in 104 additional quality-adjusted life years per 1000 patients compared to the Seventh Joint National Committee’s treatment guidelines for hypertension. The comparative health performance of the Poisson approximation was robust to the cardiovascular disease risk calculator used and calculator calibration error. Limitations: Our results are based on Markov chain modeling. Conclusions: Poisson model approximation for blood pressure treatment planning has high fidelity to optimal MDP treatment policies, which can improve usability and enhance transparency of more personalized treatment policies.
Upper extremity recovery after ischaemic and haemorrhagic stroke: Part of the SALGOT study
Authors: Persson Hanna C , Opheim Arve , Lundgren-Nilsson Åsa , Alt Murphy Margit , Danielsson Anna , Sunnerhagen Katharina S
Published: October 2016

Introduction

The purpose was to explore if there are differences in extent of change in upper extremity motor function and activity capacity, in persons with ischaemic versus haemorrhagic stroke, during the first year post stroke.

Patients and methods

One hundred seventeen persons with stroke (ischaemic n = 98, haemorrhagic n = 19) and reduced upper extremity function 3 days after onset were consecutively included to the Stroke Arm Longitudinal Study at the University of Gothenburg (SALGOT) from a stroke unit. Upper extremity motor function (Fugl-Meyer Assessment Scale for Upper Extremity (FMA-UE)) and activity capacity (Action Research Arm Test (ARAT)) were assessed at 6 assessments during the first year; age and initial stroke severity were recorded. Differences between groups in extent of change over time of upper extremity motor function and activity capacity were analysed with mixed models repeated measurements method.

Results

Significant improvements were found in function and activity in both groups within the first month ( p = 0.001). Higher age and more severe stroke had a negative impact on recovery in both groups. Larger improvements of function and activity were seen in haemorrhagic stroke compared to ischaemic, both from 3 days to 3- and 12 months, and from 1 month to 3 months. Both groups reached similar levels of function and activity at 3 months post stroke.

Conclusion

Although persons with haemorrhagic stroke had initially lower scores than those with ischaemic stroke, they had a larger improvement within the first 3 months, and thereafter both groups had similar function and activity.
Metformin improves defective hematopoiesis and delays tumor formation in Fanconi anemia mice
Authors: Zhang Qing-Shuo , Tang Weiliang , Deater Matthew , Phan Ngoc , Marcogliese Andrea N. , Li Hui , Al-Dhalimy Muhsen , Major Angela , Olson Susan , Monnat Raymond J. , Grompe Markus
Published: October 2016

Key Points

The widely used diabetes drug metformin improves hematopoiesis and delays tumor formation in a preclinical murine model of FA. Metformin reduces DNA damage in human FA patient–derived cells.
Targeting BTK through microRNA in chronic lymphocytic leukemia
Authors: Bottoni Arianna , Rizzotto Lara , Lai Tzung-Huei , Liu Chaomei , Smith Lisa L. , Mantel Rose , Reiff Sean , El-Gamal Dalia , Larkin Karilyn , Johnson Amy J. , Lapalombella Rosa , Lehman Amy , Plunkett William , Byrd John C. , Blachly James S. , Woyach Jennifer A. , Sampath Deepa
Published: October 2016

Key Points

Inhibition of HDAC reverses epigenetic silencing to upregulate miRs that target BTK and suppress its downstream prosurvival signaling. We identified a rationale for the dual targeting of BTK when combined with ibrutinib and a strategy to eliminate the C481S-mutant BTK clone.
Mice lacking the conserved transcription factor Grainyhead-like 3 (Grhl3) display increased apposition of the frontal and parietal bones during embryonic development
Authors: Goldie Stephen J. , Arhatari Benedicta D. , Anderson Peter , Auden Alana , Partridge Darren D. , Jane Stephen M. , Dworkin Sebastian
Published: October 2016

Background

Increased apposition of the frontal and parietal bones of the skull during embryogenesis may be a risk factor for the subsequent development of premature skull fusion, or craniosynostosis. Human craniosynostosis is a prevalent, and often serious embryological and neonatal pathology. Other than known mutations in a small number of contributing genes, the aetiology of craniosynostosis is largely unknown. Therefore, the identification of novel genes which contribute to normal skull patterning, morphology and premature suture apposition is imperative, in order to fully understand the genetic regulation of cranial development.

Results

Using advanced imaging techniques and quantitative measurement, we show that genetic deletion of the highly-conserved transcription factor Grainyhead-like 3 (Grhl3) in mice (Grhl3
−/−
) leads to decreased skull size, aberrant skull morphology and premature apposition of the coronal sutures during embryogenesis. Furthermore, Grhl3
−/−
mice also present with premature collagen deposition and osteoblast alignment at the sutures, and the physical interaction between the developing skull, and outermost covering of the brain (the dura mater), as well as the overlying dermis and subcutaneous tissue, appears compromised in embryos lacking Grhl3. Although Grhl3
−/−
mice die at birth, we investigated skull morphology and size in adult animals lacking one Grhl3 allele (heterozygous; Grhl3
+/−
), which are viable and fertile. We found that these adult mice also present with a smaller cranial cavity, suggestive of post-natal haploinsufficiency in the context of cranial development.

Conclusions

Our findings show that our Grhl3 mice present with increased apposition of the frontal and parietal bones, suggesting that Grhl3 may be involved in the developmental pathogenesis of craniosynostosis.
Effects of recall time on cause-of-death findings using verbal autopsy: empirical evidence from rural South Africa
Authors: Hussain-Alkhateeb Laith , Petzold Max , Collinson Mark , Tollman Stephen , Kahn Kathleen , Byass Peter
Published: October 2016

Background

Verbal autopsy (VA) is a widely used technique for assigning causes to non-medically certified deaths using information gathered from a close caregiver. Both operational and cultural factors may cause delays in follow-up of deaths. The resulting time lag—from death to VA interview—can influence ways in which terminal events are remembered, and thus affect cause-of-death assignment. This study investigates the impact of recall period on causes of death determined by VA.

Methods

A total of 10,882 deaths from the Agincourt Health and Demographic Surveillance System (HDSS) with complete VAs, including recall period, were incorporated in this study. To measure seasonal effect, cause specific mortality fractions (CSMFs) were calculated and compared by every cause for VAs undertaken within six months of death and those undertaken from six to 12 months of death. All causes were classified into eight broad categories and entered in a multiple logistic regression to explore outcome by recall period in relation to covariates.

Results

The majority of deaths (83 %) had VAs completed within 12 months. There was a tendency towards longer recall periods for deaths of those under one year or over 65 years of age. Only the acute respiratory, diarrhoeal and other unspecified non-communicable disease groups showed a CSMF ratio significantly different from unity at the 99 % confidence level between the two recall periods. Only neonatal deaths showed significantly different OR for recall exceeding 12 months (OR 1.69; p value = 0.004) and this increased when adjusting for background factors (OR 2.58; p value = 0.000).

Conclusion

A recall period of up to one year between death and VA interview did not have any consequential effects on the cause-of-death patterns derived, with the exception of neonatal causes. This is an important operational consideration given the planned widespread use of the VA approach in civil registration, HDSS sites and occasional surveys.
Medicare D Subsidies and Racial Disparities in Persistence and Adherence With Hormonal Therapy
Authors: Biggers Alana , Shi Yushu , Charlson John , Smith Elizabeth C. , Smallwood Alicia J. , Nattinger Ann B. , Laud Purushottam W. , Neuner Joan M.
Published: October 2016

Purpose

To investigate the role of out-of-pocket cost supports through the Medicare Part D Low-Income Subsidy on disparities in breast cancer hormonal therapy persistence and adherence by race or ethnicity.

Methods

A nationwide cohort of women age ≥ 65 years with a breast cancer operation between 2006 and 2007 and at least one prescription filled for oral breast cancer hormonal therapy was identified from all Medicare D enrollees. The association of race or ethnicity with nonpersistence (90 consecutive days with no claims for a hormonal therapy prescription) and nonadherence (medication possession rate < 80%) was examined. Survival analyses were used to account for potential differences in age, comorbidity, or intensity of other treatments.

Results

Among the 25,111 women in the study sample, 77% of the Hispanic and 70% of the black women received a subsidy compared with 21% of the white women. By 2 years, 69% of black and 70% of Hispanic patients were persistent compared with 61% of white patients. In adjusted analyses, patients in all three unsubsidized race or ethnicity groups had greater discontinuation than subsidized groups (white patients: hazard ratio [HR], 1.83; 95% CI, 1.70 to 1.95; black patients: HR, 2.09; 95% CI, 1.73 to 2.51; Hispanic patients: HR, 3.00; 95% CI, 2.37 to 3.89). Racial or ethnic persistence disparities that were present for unsubsidized patients were not present or reversed among subsidized patients. All three subsidized race or ethnicity groups also had higher adherence than all three unsubsidized groups, although with the smallest difference occurring in black women.

Conclusion

Receipt of a prescription subsidy was associated with substantially improved persistence to breast cancer hormonal therapy among white, black, and Hispanic women and lack of racial or ethnic disparities in persistence. Given high subsidy enrollment among black and Hispanic women, policies targeted at low-income patients have the potential to also substantially reduce racial and ethnic disparities.
Addition of Androgens Improves Survival in Elderly Patients With Acute Myeloid Leukemia: A GOELAMS Study
Authors: Pigneux Arnaud , Béné Marie C. , Guardiola Philippe , Recher Christian , Hamel Jean-Francois , Sauvezie Mathieu , Harousseau Jean-Luc , Tournilhac Olivier , Witz Francis , Berthou Christian , Escoffre-Barbe Martine , Guyotat Denis , Fegueux Nathalie , Himberlin Chantal , Hunault Mathilde , Delain Martine , Lioure Bruno , Jourdan Eric , Bauduer Frederic , Dreyfus Francois , Cahn Jean-Yves , Sotto Jean-Jacques , Ifrah Norbert
Published: October 2016

Purpose

Elderly patients with acute myeloid leukemia (AML) have a poor prognosis, and innovative maintenance therapy could improve their outcomes. Androgens, used in the treatment of aplastic anemia, have been reported to block proliferation of and initiate differentiation in AML cells. We report the results of a multicenter, phase III, randomized open-label trial exploring the benefit of adding androgens to maintenance therapy in patients 60 years of age or older.

Patients and Methods

A total of 330 patients with AML de novo or secondary to chemotherapy or radiotherapy were enrolled in the study. Induction therapy included idarubicin 8 mg/m2 on days 1 to 5, cytarabine 100 mg/m2 on days 1 to 7, and lomustine 200 mg/m2 on day 1. Patients in complete remission or partial remission received six reinduction courses, alternating idarubicin 8 mg/m2 on day 1, cytarabine 100 mg/m2 on days 1 to 5, and a regimen of methotrexate and mercaptopurine. Patients were randomly assigned to receive norethandrolone 10 or 20 mg/day, according to body weight, or no norethandrolone for a 2-year maintenance therapy regimen. The primary end point was disease-free survival by intention to treat. Secondary end points were event-free survival, overall survival, and safety. This trial was registered at www.ClinicalTrials.gov identifier NCT00700544.

Results

Random assignment allotted 165 patients to each arm; arm A received norethandrolone, and arm B did not receive norethandrolone. Complete remission or partial remission was achieved in 247 patients (76%). The Schoenfeld time-dependent model showed that norethandrolone significantly improved survival for patients still in remission at 1 year after induction. In arms A and B, respectively, 5-year disease-free survival was 31.2% and 16.2%, event-free survival was 21.5% and 12.9%, and overall survival was 26.3% and 17.2%. Norethandrolone improved outcomes irrelevant to all prognosis factors. Only patients with baseline leukocytes > 30 × 109/L did not benefit from norethandrolone.

Conclusion

This study demonstrates that maintenance therapy with norethandrolone significantly improves survival in elderly patients with AML without increasing toxicity.
Relapsed or Refractory Double-Expressor and Double-Hit Lymphomas Have Inferior Progression-Free Survival After Autologous Stem-Cell Transplantation
Authors: Herrera Alex F. , Mei Matthew , Low Lawrence , Kim Haesook T. , Griffin Gabriel K. , Song Joo Y. , Merryman Reid W. , Bedell Victoria , Pak Christine , Sun Heather , Paris Tanya , Stiller Tracey , Brown Jennifer R. , Budde Lihua E. , Chan Wing C. , Chen Robert , Davids Matthew S. , Freedman Arnold S. , Fisher David C. , Jacobsen Eric D. , Jacobson Caron A. , LaCasce Ann S. , Murata-Collins Joyce , Nademanee Auayporn P. , Palmer Joycelynne M. , Pihan German A. , Pillai Raju , Popplewell Leslie , Siddiqi Tanya , Sohani Aliyah R. , Zain Jasmine , Rosen Steven T. , Kwak Larry W. , Weinstock David M. , Forman Stephen J. , Weisenburger Dennis D. , Kim Young , Rodig Scott J. , Krishnan Amrita , Armand Philippe
Published: October 2016

Purpose

Double-hit lymphomas (DHLs) and double-expressor lymphomas (DELs) are subtypes of diffuse large B-cell lymphoma (DLBCL) associated with poor outcomes after standard chemoimmunotherapy. Data are limited regarding outcomes of patients with relapsed or refractory (rel/ref) DEL or DHL who undergo autologous stem-cell transplantation (ASCT). We retrospectively studied the prognostic impact of DEL and DHL status on ASCT outcomes in patients with rel/ref DLBCL.

Methods

Patients with chemotherapy-sensitive rel/ref DLBCL who underwent ASCT at two institutions and in whom archival tumor material was available were enrolled. Immunohistochemistry for MYC, BCL2, and BCL6 and fluorescence in situ hybridization (FISH) for MYC were performed. In cases with MYC rearrangement or copy gain, FISH for BCL2 and BCL6 was also performed.

Results

A total of 117 patients were included; 44% had DEL and 10% had DHL. DEL and DHL were associated with inferior progression-free survival (PFS), and DHL was associated with poorer overall survival (OS). The 4-year PFS in patients with DEL compared with those with non-DEL was 48% versus 59% ( P = .049), and the 4-year OS was 56% versus 67% ( P = .10); 4-year PFS in patients with DHL compared with those with non-DHL was 28% versus 57% ( P = .013), and 4-year OS was 25% versus 61% ( P = .002). The few patients with concurrent DEL and DHL had a poor outcome (4-year PFS, 0%). In multivariable models, DEL and DHL were independently associated with inferior PFS, whereas DHL and partial response ( v complete response) at transplant were associated with inferior OS.

Conclusion

DEL and DHL are both associated with inferior outcomes after ASCT in patients with rel/ref DLBCL. Although ASCT remains a potentially curative approach, these patients, particularly those with DHL, are a high-risk subset who should be targeted for investigational strategies other than standard ASCT.
Body Composition Changes After Very-Low-Calorie Ketogenic Diet in Obesity Evaluated by 3 Standardized Methods
Authors: Gomez-Arbelaez Diego , Bellido Diego , Castro Ana I. , Ordoñez-Mayan Lucia , Carreira Jose , Galban Cristobal , Martinez-Olmos Miguel A. , Crujeiras Ana B. , Sajoux Ignacio , Casanueva Felipe F.
Published: October 2016

Context:

Common concerns when using low-calorie diets as a treatment for obesity are the reduction in fat-free mass, mostly muscular mass, that occurs together with the fat mass (FM) loss, and determining the best methodologies to evaluate body composition changes.

Objective:

This study aimed to evaluate the very-low-calorie ketogenic (VLCK) diet-induced changes in body composition of obese patients and to compare 3 different methodologies used to evaluate those changes.

Design:

Twenty obese patients followed a VLCK diet for 4 months. Body composition assessment was performed by dual-energy X-ray absorptiometry (DXA), multifrequency bioelectrical impedance (MF-BIA), and air displacement plethysmography (ADP) techniques. Muscular strength was also assessed. Measurements were performed at 4 points matched with the ketotic phases (basal, maximum ketosis, ketosis declining, and out of ketosis).

Results:

After 4 months the VLCK diet induced a −20.2 ± 4.5 kg weight loss, at expenses of reductions in fat mass (FM) of −16.5 ± 5.1 kg (DXA), −18.2 ± 5.8 kg (MF-BIA), and −17.7 ± 9.9 kg (ADP). A substantial decrease was also observed in the visceral FM. The mild but marked reduction in fat-free mass occurred at maximum ketosis, primarily as a result of changes in total body water, and was recovered thereafter. No changes in muscle strength were observed. A strong correlation was evidenced between the 3 methods of assessing body composition.

Conclusion:

The VLCK diet-induced weight loss was mainly at the expense of FM and visceral mass; muscle mass and strength were preserved. Of the 3 body composition techniques used, the MF-BIA method seems more convenient in the clinical setting.
The impact of FOXO on dopamine and octopamine metabolism in Drosophila under normal and heat stress conditions
Authors: Gruntenko Nataly E. , Adonyeva Natalya V. , Burdina Elena V. , Karpova Evgenia K. , Andreenkova Olga V. , Gladkikh Daniil V. , Ilinsky Yury Y. , Rauschenbach Inga Yu
Published: October 2016

The forkhead boxO transcription factor (FOXO) is a component of the insulin signalling pathway and plays a role in responding to adverse conditions, such as oxidative stress and starvation. In stressful conditions, FOXO moves from the cytosol to the nucleus where it activates gene expression programmes. Here, we show that FOXO in Drosophila melanogaster responds to heat stress as it does to other stressors. The catecholamine signalling pathway is another component of the stress response. In Drosophila, dopamine and octopamine levels rise steeply under heat, nutrition and mechanical stresses, which are followed by a decrease in the activity of synthesis enzymes. We demonstrate that the nearly twofold decline of FOXO expression in foxoBG01018 mutants results in dramatic changes in the metabolism of dopamine and octopamine and the overall response to stress. The absence of FOXO increases tyrosine decarboxylase activity, the first enzyme in octopamine synthesis, and decreases the enzymatic activity of enzymes in dopamine synthesis, alkaline phosphatase and tyrosine hydroxylase, in young Drosophila females. We identified the juvenile hormone as a mediator of FOXO regulation of catecholamine metabolism. Our findings suggest that FOXO is a possible trigger for endocrinological stress reactions.
Neural specificity of the RNA-binding protein Elav is achieved by post-transcriptional repression in non-neural tissues
Authors: Sanfilippo Piero , Smibert Peter , Duan Hong , Lai Eric C.
Published: October 2016

Drosophila Elav is the founding member of the conserved family of Hu RNA-binding proteins (RBPs), which play crucial and diverse roles in post-transcriptional regulation. Elav has long served as the canonical neuronal marker. Surprisingly, although Elav has a well-characterized neural cis-regulatory module, we find endogenous Elav is also ubiquitously transcribed and post-transcriptionally repressed in non-neural settings. Mutant clones of multiple miRNA pathway components derepress ubiquitous Elav protein. Our re-annotation of the elav transcription unit shows not only that it generates extended 3′ UTR isoforms, but also that its universal 3′ UTR isoform is much longer than previously believed. This longer common 3′ UTR includes multiple conserved, high-affinity sites for the miR-279/996 family. Of several miRNA mutants tested, endogenous Elav and a transgenic elav 3′ UTR sensor are derepressed in mutant clones of mir-279/996. We also observe cross-repression of Elav by Mei-P26, another RBP derepressed in non-neural miRNA pathway clones. Ubiquitous Elav has regulatory capacity, since derepressed Elav can stabilize an Elav-responsive sensor. Repression of Elav in non-neural territories is crucial as misexpression here has profoundly adverse consequences. Altogether, we define unexpected post-transcriptional mechanisms that direct appropriate cell type-specific expression of a conserved neural RBP.
Control of apico–basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7
Authors: Noordstra Ivar , Liu Qingyang , Nijenhuis Wilco , Hua Shasha , Jiang Kai , Baars Matthijs , Remmelzwaal Sanne , Martin Maud , Kapitein Lukas C. , Akhmanova Anna
Published: October 2016

The microtubule cytoskeleton regulates cell polarity by spatially organizing membrane trafficking and signaling processes. In epithelial cells, microtubules form parallel arrays aligned along the apico–basal axis, and recent work has demonstrated that the members of CAMSAP/Patronin family control apical tethering of microtubule minus ends. Here, we show that in mammalian intestinal epithelial cells, the spectraplakin ACF7 (also known as MACF1) specifically binds to CAMSAP3 and is required for the apical localization of CAMSAP3-decorated microtubule minus ends. Loss of ACF7 but not of CAMSAP3 or its homolog CAMSAP2 affected the formation of polarized epithelial cysts in three-dimensional cultures. In short-term epithelial polarization assays, knockout of CAMSAP3, but not of CAMSAP2, caused microtubule re-organization into a more radial centrosomal array, redistribution of Rab11-positive (also known as Rab11A) endosomes from the apical cell surface to the pericentrosomal region and inhibition of actin brush border formation at the apical side of the cell. We conclude that ACF7 is an important regulator of apico–basal polarity in mammalian intestinal cells and that a radial centrosome-centered microtubule organization can act as an inhibitor of epithelial polarity.
Public associations as an effective tool for Political and Legal socialization of youth
Authors: Щеголева  , Shchegoleva Natalya , Щеголев  , Shchegolev Sergey
Published: October 2016

The article analyzes the process of political and legal socialization. The necessity to prioritize the for-mation of active life position of today´s youth with the aim of building a civil society and rule of law. The authors emphasize the advisability of not only development, but the effective implementation of the civil society and public authorities versatile system of measures aimed at its support and development of youth associations.
Trust between government and business - basis of formation of business climate in the region
Authors: Музалевский  , Muzalevskiy L.
Published: October 2016

The article deals with the directions of the formation of business climate in the region. The presence of specific conditions and factors of development of small and medium business in the region is substantiated that involves the use of a differentiated policy of the state to create favorable business climate.
Programming and budgeting system: foreign practice and the basis for practical application for Russia
Authors: Брузати  , Bruzati Luka , Грядунова  , Gryadunova Alina
Published: October 2016

The main idea of the article is reforming of the public administration system and correct using of the fi-nance. The way of reforming is Programming and Budgeting System, it has a lot of advantages and provides the result. A lot of foreign countries use this system very successfully and this practice can be use in Russia with some correction. The USA is the first country which started to use this system. There are some prob-lems in sphere of using Programming and Budgeting System and it has to solve them because it is very per-spective and useful direction. It is necessary to use different programs of the development and plan costs for them, then it is important to set clear goals and encourage employees for achievement of them.
On the strategy of social and economic development of regions (on the example of the central federal district)
Authors: Мурашов  , Murashov A.
Published: October 2016

The article proves the importance of the strategic approach to the management of the economy of the region; a comparative analysis of the strategy objectives and priorities of socio-economic development of the regions of the Central Federal District of Russia is presented; it is concluded that competitiveness of the regions and of Russia as a whole depends on their ability to implement the strategy of their social and economic development.
Features of the development of the russian insurance market at the present stage
Authors: Мироненко  , Mironenko A.
Published: October 2016

The article describes the features of the development of market of insurance compa-nies; specifics of positioning in modern conditions arehighlighted. The author marked the most ac-tive administrative business processes in management system of insurance companies: manufacturing, marketing and informational.
Professional health of medical workers as a factor of the quality of medical service in public health
Authors: Игнатова  , Ignatova Tatyana , Еремин  , Eremin O.
Published: October 2016

The article deals with the concept of quality of medical care, basic health indicators of professional health of medical workers. The current results of diagnostics of job satisfaction by health workers according to qualitative and quantitative characteristics are presented. Some objective and subjective factors influencing the formation of professional health of medical workers are revealed. The article determines the social status of the person engaged in the field of medicine and the place of medical profession in the system of social relations in modern conditions, when the profession of medical worker is characterized by versatility.
Efficiency of the activity of the highest official of the subject of the russian federation: criteria of the assessment
Authors: Денисова  , Denisova Olga
Published: October 2016

In the article the author analyzes the process of efficiency assessment of the activity of the highest official of the subject of the Russian Federation. The attention is focused on inadequacy of application of exclusively quantitative indices. Based on the analysis of expansion practice of assessment criteria of efficiency, the need of public participation in rating process is shown. In this context the activity of Fund of development of civil society is examined as the center which created the clear and acceptable system of rating indicators of effi-ciency of the highest officials.
To the question of prestige of municipal service
Authors: Пономаренко  , Ponomarenko N.
Published: October 2016

This article addresses the issue of the prestige of municipal service, the ways to increase the image of municipal service as a whole. The question of the influence of mass media on public opinion on the municipal service is discussed. The issue of the negative influence of corruption on the prestige of municipal service is touched upon.
In silico and in vivo wound healing studies of ursolic acid isolated from Clematis gouriana against GSK-3 beta
Authors: NAIKA H. RAJA , BHAVANA S. , TEIXEIRA DA SILVA JAIME A. , LINGARAJU K. , MOHAN VIVEK CHANDRA , KRISHNA V.
Published: October 2016

Abstract. Naika HR, Bhavana S, Teixeira da Silva JA, Lingaraju K, Mohan VC, Krishna V. 2016. In silico and in vivo wound healing studies of ursolic acid isolated from Clematis gouriana against GSK-3 beta. Nusantara Bioscience 8: 232-244. Clematis gouriana Roxb. (Ranunculaceae) is an endemic medicinal plant of Western Ghats. Ursolic acid (UA) was isolated from the methanolic extract (ME), which was characterized by spectral studies, and was subjected to in silico studies on Glycogen synthase kinase 3-β (GSK3-β) protein, to inhibit protein function. UA showed least binding energy (-13.457 ΔG) towards the binding site and molecular dynamics studies showed minimum potential energy and greater stability with target protein. Thus, UA may be considered as a potential inhibitor of the GSK3-β protein. The wound-healing activity was assessed by excision, incision, and dead space wound models on Wistar strain rats. The results of excision, incision, and dead space wound models showed significant activity. There was a significant increase in skin-breaking strength in rats treated with UA (562.36 ± 7.60 g). The effects of ME and UA on the dead space wound model were pronounced, with a significant increase in the weight of granulation tissue (26.33 ± 0.25 mg), tensile strength (647.00 ± 0.71 g), and hydroxyproline content (1793.83 ± 0.64 µg/100 g). Thus, UA-treated animals showed excellent wound-healing activity. Animals treated with nitrofurazone, ME and UA showed increased collagenation and decreased accumulation of macrophages at the site of injury.
El discurso pedagógico en la fiesta de la ciencia en la universidad deseada: España 1900-1936
Authors: Hernández Díaz José María
Published: October 2016

En los inicios del siglo XX la Universidad española ofrece un panorama esperanzador sobre su proceso de modernización científica y docente, tal como lo habían intuido Giner de los Ríos y la Institución Libre de Enseñanza. Era la universidad deseada, como contrapunto a un modelo de universidad anacrónica que se había heredado del siglo XIX. El artículo busca explicar este proceso mediante el análisis de los discursos pronunciados en la llamada Fiesta de la Ciencia.
La literatura doctrinal y devocional en las escuelas del Barroco español. El Memento mori como materia escolar
Authors: Salido López José Vicente
Published: October 2016

La influencia de la Contrarreforma es fundamental para entender los modelos educativos de finales del siglo xvi y del Barroco. La formación religiosa se convirtió en el principal objetivo de las escuelas de primeras letras, de ahí que los materiales para la lectura y escritura contaran con abundantes textos extraídos de obras doctrinales y devocionales. En el Barroco, por su particular visión de la vida, se enriquecen con reflexiones sobre la muerte y la vanidad mundana. El presente artículo pretende analizar los principales manuales de caligrafía de la época para constatar esta finalidad moralizante en los ejercicios de muestra para alumnos, rastreando las fuentes y el principio pedagógico que subyace en sus autores.
O TRABALHO DE PROFESSORES DA ESCOLA PÚBLICA NITEROIENSE: LIMITES DA PRECARIZAÇÃO OU PRECARIZAÇÃO SEM LIMITES
Authors: Rodrigues Jéssica Do Nascimento , Rangel Mary
Published: October 2016

Neste artigo, recorte de uma tese de doutorado em Educação, objetivou-se perscrutar o contexto de atuação dos professores de Língua Portuguesa de três escolas municipais de Niterói-RJ, procurando entender as condições concretas de realização de seu trabalho, seus limites e suas possibilidades, de acordo com as versões da realidade produzidas pelos próprios professores. Para tanto, no referencial teórico, lançou-se mão do materialismo histórico e dialético e da teoria da enunciação, de fundamentação bakhtiniana, os quais também guiaram a metodologia adotada. Como dispositivos enunciativos, utilizaram-se questionários semiestruturados, grupos focais e entrevistas individuais. Como resultado, destacou-se o panorama de sucateamento das escolas públicas e de precarização das condições de vida e de trabalho dos docentes, decorrente do próprio acirramento das relações materiais e não materiais na realidade em que atuam.
A EDUCAÇÃO A DISTÂNCIA E A FORMAÇÃO DE PROFESSORES EM LICENCIATURAS NA UFT
Authors: Faria Denilda Caetano , Almeida Maria Zeneide Carneiro Magalhães , Silva Moisés Gregório Gregório
Published: October 2016

Neste estudo apresentam-se algumas considerações sobre a formação docente a distância, em contexto de expansão e interiorização da educação superior. Buscam-se, a partir da história e da formação a distância, compreender e refletir sobre as mudanças e as transformações no âmbito das políticas de formação de professores nas IES e suas implicações, mais especificamente na UFT. A metodologia adotada recorre às fontes bibliográficas, documentais e entrevistas. Os resultados evidenciam que houve um aumento significativo de oferta de cursos de licenciaturas a distância no Tocantins, contudo muitos desafios precisam ser superados no sentido de ampliar o acesso e garantir a qualidade na formação docente.
PROFESSOR REFLEXIVO: UM CONTRIBUTO À EPISTEMOLOGIA DA PRÁTICA E À FORMAÇÃO DOCENTE
Authors: Lira Mirtes Ribeiro de , Sarmento Elisângela Campos Damasceno
Published: October 2016

Este artigo tem por objetivo apresentar algumas considerações sobre o professor reflexivo como impulso à epistemologia da prática, com vistas a subsidiar a formação permanente dos professores, condição indispensável à conquista da qualidade do exercício profissional. O presente trabalho fundamenta-se, primordialmente, em Dewey (1960), Freire (1997), Schön (2000), Pimenta (2002) e Zapata (2004). A metodologia da pesquisa pauta-se em relatos de experiência docente, assentados numa revisão bibliográfica. Sendo assim, espera-se que os resultados obtidos possam evidenciar a relevância do professor reflexivo e da epistemologia da prática no contexto escolar, tendo em vista as contribuições à formação docente e à consequente melhoria dos processos de ensino e aprendizagem.
SOB A CÚPULA DAS CONSTELAÇÕES: A TEORIA ATOR-REDE E O ENSINO DE ASTRONOMIA NO PLANETÁRIO
Authors: Gonçalves Erica De Oliveira , Borges Martha Kaschny
Published: October 2016

A abrangência e a magnitude dos conteúdos de astronomia despertam a curiosidade de crianças e de adultos, contribuem para a produção de conhecimentos e podem estimular o desenvolvimento de práticas docentes interdisciplinares. Nesse contexto, os Planetários oferecem possibilidades diferenciadas para os processos de ensino e de aprendizagem da astronomia. O objetivo desse artigo é analisar como os professores dos anos iniciais do ensino fundamental percebem o Planetário nos processos educativos. Para isto utilizamos o quadro teórico e metodológico da Teoria Ator-Rede (TAR) proposto por Bruno Latour (2012), especialmente a partir do conceito de actante, que pode ser um agente humano ou não humano. Os actantes podem assumir dois papéis distintos: de mediador, quando suas ações/associações na rede promovem mudanças; ou o de intermediário, quando o agente apenas transmite/reproduz as ações/associações existentes sem modificar as redes e os outros actantes. Assim, nos interessa responder à seguinte perguntanorteadora: em quais situações os professores concebem o Planetário como mediador dos processos de ensino e aprendizagem de astronomia ou como intermediário destes processos? Inicialmente, com o intuito de aproximação à temática, realizamos uma análise dos Parâmetros Curriculares Nacionais (PCN) sobre o ensino de astronomia. A seguir, examinamos 97 questionários dos professores dos diferentes níveis de ensino que frequentaram o Planetário com suas turmas durante o ano de 2014. Os resultados indicam que os professores dos anos iniciais, ao agirem de forma planejada e intencional no ensino de conteúdos de astronomia, percebem o Planetário como mediador, segundo a Teoria Ator-Rede, uma vez que, nestas situações, o Planetário - um dispositivo não humano – influencia, modifica e organiza as ações de ensino e aprendizagem de astronomia.
Investigación: Fundamentos teóricos de la corriente de investigación sobre relevancia valorativa Principales contribuciones de la investigación sobre intangibles orientada hacia el mercado de capitales
Authors: Ficco Cecilia Rita
Published: October 2016

El presente trabajo tiene por objetivo exponer y analizar los principalesfundamentos teóricos y conceptuales de la corriente de investigaciónsobre relevancia valorativa, contextualizándola dentro de la investigaciónorientada al mercado de capitales y del paradigma utilitarista, asícomo presentar algunos de los principales resultados que se han logradoa través de las investigaciones que, enmarcadas en dicha corriente, hanexaminado la relevancia valorativa de los intangibles. Metodológicamente,se ha realizado una investigación descriptiva y de tipo documental. Seconcluye que la corriente de investigación sobre relevancia valorativa, queencuentra en el modelo de Ohlson (1995) un sólido fundamento teórico,tiene dentro de sus líneas más prolíficas y con mayores perspectivas afuturo, a aquella tendiente a demostrar la relevancia valorativa de losintangibles en los mercados de capitales. Los resultados de las investigacionesrealizadas en esta línea han aportado evidencias claras acercade la importancia de los intangibles para la valoración de las empresas,los que resultan de sumo interés tanto para el ámbito académico comopara los organismos emisores de normas contables, ya que proporcionan una base sólida para decidir qué información debe ser incluida en losestados financieros con el fin de incrementar su utilidad, lo que resultafundamental para la mejora del modelo contable actual.
Divulgación: Estudios superiores y trabajo: implicancias de su simultaneidad
Authors: Pacífico Andera , Gandolfo Pablo
Published: October 2016

El objetivo de este artículo es determinar cómo la simultaneidad entre estudiossuperiores y trabajo inciden en las trayectorias de los estudiantes avanzadosde la Facultad de Ciencias Económicas de la Universidad Nacionaldel Litoral (FCE–UNL). Para cumplir con este objetivo, se utilizó informaciónde una encuesta realizada en 2015 a los alumnos de la FCE–UNL, junto condatos oficiales de dicha institución. Este estudio muestra que los trabajosque los alumnos poseen, en su gran mayoría, están muy relacionados consus estudios y que la cantidad de horas trabajadas excede ampliamentelo recomendado. La alta relación entre estudios y trabajo actúa como unfactor motivador dándole la oportunidad al alumno de apropiarse significativamentede los contenidos. También permite que el estudiante desarrolleun aprendizaje integral, mejorando sus oportunidades laborales futuras alcontar con experiencia previa en la profesión. Sin embargo, la gran cantidadde horas que trabajan estos estudiantes puede lentificar la finalización desus estudios y en algunos casos este rezago puede derivar en el abandono.
Elite bilingual education in Brazil: an applied linguist’s perspective
Authors: Liberali Fernanda Coelho , Megale Antonieta
Published: October 2016

We have live a historical moment when superdiversity creates a composition and affluence of importante variables that affect where, how and who people live with. In this scenario, issues of a multilingual formation in different countries are intensified and so is the establishent of elite bilingual schools. This article aims to present the relationship between the increasing phenomenon of Elite Bilingual Education in Brazil and studites developed in this field by Applied Linguistics. It is organized for a presentation of the globalizing multicultural context which supports the interest in the area and for a discussion of its connection with bilingual education. It will also discuss nation-wide circulating definitions of Bilingual Education and Elite Bilingual Education. Moreover, it will approach research and activities carried out in the Brazilian context, through the presentaion of numbers of schools and research in the area, besindes some researhers and their institutions involved with the issue. Furthermore, the article will clarify the scenario and the situation in Brazil. It will also include a specific focus on research being conducted at present at PUC-SP. Finally, some gaps, defined in national research and related to international ones, will be pointed out.