bluuebunny/crossref_metadata_2025 huggingface dataset:
Efektifitas Progressive Muscle Relaxation Techniques Terhadap Kecemasan Pada Pasien Skizofrenia: Literature Review
Authors: Maulana Indra Maulana , Hesti Platini  , Hendrawati  , Iceu Amira
Published: January 2024

Skizofrenia menempati urutan ketiga di beban penyakit gangguan jiwa Indonesia tahun 2017. Prevalensi kecemasan pada pasien skizofrenia cukup tinggi dan memengaruhi gejala psikotik mereka. Skizofrenia adalah gangguan psikotik yang terdiri dari beberapa gejala positif dan negative. Skizofrenia merupakan gangguan psikotik yang tanda utamanya adalah gangguan pikiran, emosi, dan perilaku, yang mana pemikiran tersebut tidak saling berhubungan secara logis, persepsi yag keliru, afek datar, dan gangguan aktifitas motorik.Tujuan dari penelitian ini yaitu untuk mengetahui efektifitas relaksasi otot progresif terhadap kecemasan pada pasien skizofrenia. Metode dalam penelitian ini yaitu literature review dari database Google Scholar dan PubMed dengan kriteria 10 tahun terakhir, full text, yang membahas relaksasi otot progresif, kecemasan, dan skizofrenia. Hasil analisis dari 4 artikel didapatkan bahwa relaksasi otot progresif terbukti efektif untukmenurunkan kecemasan, stres, gejala psikotik dan meningkatkan kualitas hidup pasien skizofrenia. Namun efek penggunaan intervensi ini masih dalam jangka pendek jika digunakan pada pasien skizofrenia kronis, sehingga diperlukan latihan yang lebih lama untuk mendapatkan efek jangka Panjang. Terapi utama pasien skizofrenia yaitu melalui terapi farmakologi, namun penggunaan jangka panjang terapi tersebut memberikan efek samping yang dapat menurunkan kualitas hidup pasien. Oleh karena itu, penggunaan terapi nonfarmakologi pada pasien skizofrenia penting dilakukan, terapi nonfarmakologi yang sering digunakan yaitu relaksasi otot progresif untuk menurunkan kecemasan
Goddess of the Jewelled Web – Visionary Arts’ Connection to Truth
Authors: Mirante Daniel
Published: January 2024

Art striving for an existential veracity or ‘truth’ will be put upon a difficult and confusing road, forking and branching pathways; between on one hand positivist and nominalist systems tending toward rationality (as in ‘ratio’, to measure), and on the other extreme something akin to pure aesthetics, decor, and the enjoyment of a sensoric object. What we wish to seek here is a deeper meaning for the Arts which put Sacred Art upon a respectable and integral foundation.
ADAPTAÇÃO CRIATIVA NAS IMAGENS DO TARÔ DO TERREIRO DE UMBANDA PAI MANECO
Authors: Mendes Maria Cristina
Published: January 2024

O Tarô do Terreiro Pai Maneco, cujas imagens foram criadas por mim, é composto por vinte e dois Arcanos maiores e uma carta coringa. O objetivo deste artigo é apresentar partes do processo de criação em que identifico a atuação/ inspiração espiritual. Para tanto levanto questões acerca das formas de comunicação entre sagrado e religiosidade (ELIADE e HOOKS), destaco características dos processos tradutórios criativos (CAMPOS, PLAZA e SCHNAIDERMAN) e lanço luz sobre o valor da dúvida nos processos poéticos contemporâneos (FERVENZA, SALLES e TESSLER). Indago-me acerca do transitar sígnico identificado na realização das pinturas digitais, considerando a importância de tal ato para a difusão de uma religião de base afro-indígena-brasileira. Sob a ótica de uma crítica engajada e amorosa (HOOKS), acredito aprofundar relações dialógicas com as entidades espirituais por meio da pintura e da breve interpretação das cartas. A pesquisa oriunda da criação do Tarô se justifica pelo respeito à ancestralidade mágica e valorização da diversidade de credos não hegemônicos, elementos basilares para a potencialização da abordagem decolonial na cultura e na arte brasileiras.
El espacio para generar encuentro se halla en ruinas
Authors: Perepelycia Alexis
Published: January 2024

En el panorama cultural del continente latinoamericano del siglo XXI la improvisación libre aparece como una expresión emergente con marcadas características regionales. En este artículo compartimos algunas ideas en torno a una mirada interdisciplinar sobre la improvisación libre en relación a tópicos como la auralidad, el sonido, la praxis, el referente, y aspectos provenientes de la biología, la ecología y lo ecosistémico, situándonos en el contexto socio-político latinoamericano. Elementos disímiles provenientes de diferentes disciplinas y autores/as que encontramos relevantes nos sirven aquí para tensionar el territorio de acción y problematizar de este modo temas que a nuestro entender atraviesan a la improvisación libre.
Variações híbridas no cinema documentário
Authors: De Souza Lucas Soares
Published: January 2024

Embora muitas vezes encarados como pólos opostos, o documentário e a ficção são, na verdade, teórica e praticamente entrelaçados, assim como a história e a ficção, também convencionalmente definidos como opostos, são simbioticamente ligados. O historiador Hayden White argumentou em seu livro Metahistory que a distinção mito/história é arbitrária e uma invenção recente. No que diz respeito ao cinema, bem como à escrita, White apontou que pouco importa se o mundo que é transmitido para o leitor/espectador é concebido para ser real ou imaginário, a forma de dar sentido discursivo a ele através do tropos e da montagem do enredo é idêntica (WHITE, 1973). Neste artigo, examinaremos as maneiras que a hibridação entre documentário e ficção tem sido mobilizada como radical recurso estético.
Questionnaire survey of risk factors for recurrence of ocular inflammation in patients with uveitis after SARS-CoV-2 infection
Authors: Ma Zonghui , Chi Ying , Guo Chunying , Zhang Jing , Yang Liu
Published: January 2024

Introduction

During the COVID-19 pandemic in China, the proportion of patients with uveitis who were infected with SARS-CoV-2 increased greatly. The impact of SARS-CoV-2 infection on patients with uveitis has not been fully described.

Methods

A questionnaire on SARS-CoV-2 infection was sent to patients with uveitis to assess ocular and systemic conditions before and after infection. Chi-square analysis and multifactorial regression analysis were used to investigate the associations between each risk factor and the recurrence of uveitis after SARS-CoV-2 infection.

Results

One hundred thirty-nine patients with noninfectious uveitis completed the questionnaire; 114 (82.0%) had COVID-19, and 27 (23.7%) had recurrent or exacerbated uveitis after COVID-19. There was a higher rate of recurrence or aggravation of ocular inflammation in patients who developed severe COVID-19 symptoms (severe group 8/20 vs. nonsevere group 19/94). There were significant differences in the rates of recurrence and aggravation between the two groups of patients who differed in terms of ocular inflammatory activity within 3 months prior to SARS-CoV-2 infection (χ2 = 10.701, P=0.001), as well as in the rates of recurrence and aggravation after cessation of systemic immunomodulatory therapy. After multifactorial regression analysis, patients with active ocular inflammation within 3 months prior to SARS-CoV-2 infection had a greater risk of recurrence or exacerbation of uveitis after COVID-19 (OR=4.298, P=0.002).

Conclusion

The degree of ocular inflammatory activity within 3 months prior to SARS-CoV-2 infection may be a major factor influencing the recurrence or exacerbation of uveitis after infection. Interruption of medication should be minimized in patients with unstable inflammatory control.
A meta-analysis of the therapeutic effect of probiotic intervention in obese or overweight adolescents
Authors: Duan Yuanqing , Wang Lanping , Ma Yan , Ning Lei , Zhang Xinhuan
Published: January 2024

Background & aims

Existing evidence on the possible effects of probiotics on obese or overweight adolescents has not been fully established. Therefore, the aim of this study was to explore the effects of probiotic supplementation on anthropometric indices, inflammatory markers and metabolic indices in obese or overweight adolescents.

Methods

The literature up to March 2023 related to probiotic intervention in obese or overweight adolescents was searched and screened from multiple databases, including the CNKI(China national knowledge infrastructure), CBM(Chinese biomedical literature database), PubMed, EmBase, and Cochrane library databases. All randomized controlled trials using probiotic supplements in obese or overweight adolescents were included in this systematic review and meta-analysis.

Results

A total of 8 studies that met the inclusion criteria were included in this study. There were 201 cases in the experimental group (probiotic treatment) and 190 cases in the control group. Compared to the control group, probiotic intervention in adolescents resulted in a decrease in body mass index, fasting blood glucose and C-reactive protein with WMD(Weighted mean difference) and 95% CI of -2.53 (-4.8 to -0.26) kg/m2, -0.80 (-1.13 to -0.47) mol/L and -0.24 (-0.43 to -0.05) mg/L, respectively. No significant changes were found in weight, waist circumference, waist-to-hip ratio, insulin, Homeostatic Model Assessment of insulin resistance, interleukin 6, tumor necrosis factor alpha and so on; however, an unfavorable elevated effect in total cholesterol, triglycerides, and low-density lipoproteins was detected with WMD and 95% CI of 0.06 (0.02 to 0.09) mmol/L, 0.18 (0.14 to 0.21) mmol/L, and 0.19 (0.18 to 0.20) mmol/L, respectively.

Conclusion

According to our results, probiotic supplementation was beneficial in managing metabolic indicators such as fasting blood glucose, body mass index and inflammation-related C-reactive protein in overweight or obese adolescents. Further large scale studies are warranted to confirm present findings and to identify the effects and mechanisms to provide more precise evidence for clinical intervention.

Systematic review registration

doi: 10.37766/inplasy2024.1.0081, identifier INPLASY202410081.
Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses
Authors: Ren Wanlu , Wang Jianwen , Zeng Yaqi , Wang Tongliang , Meng Jun , Yao Xinkui
Published: January 2024

Introduction

The Kazakh horse, renowned for its excellence as a breed, exhibits distinctive reproductive traits characterized by early maturity and seasonal estrus. While normal reproductive function is crucial for ensuring the breeding and expansion of the Kazakh horse population, a noteworthy decline in reproductive capabilities is observed after reaching 14 years of age.

Methods

In this study, ovarian granulosa cells (GCs) were meticulously collected from Kazakh horses aged 1, 2, 7, and above 15 years old (excluding 15 years old) for whole transcriptome sequencing.

Results

The analysis identified and selected differentially expressed mRNAs, lncRNAs, miRNAs, and circRNAs for each age group, followed by a thorough examination through GO enrichment analysis. The study uncovered significant variations in the expression profiles of mRNAs, lncRNAs, miRNAs, and circRNAs within GCs at different stages of maturity. Notably, eca-miR-486-3p and miR-486-y exhibited the highest degree of connectivity. Subsequent GO, KEGG, PPI, and ceRNA network analyses elucidated that the differentially expressed target genes actively participate in signaling pathways associated with cell proliferation, apoptosis, and hormonal regulation. These pathways include but are not limited to the MAPK signaling pathway, Hippo signaling pathway, Wnt signaling pathway, Calcium signaling pathway, Aldosterone synthesis and secretion, Cellular senescence, and NF-kappa B signaling pathway—essentially encompassing signal transduction pathways crucial to reproductive processes.

Discussion

This research significantly contributes to unraveling the molecular mechanisms governing follicular development in Kazakh horses. It establishes and preliminarily validates a differential regulatory network involving lncRNA-miRNA-mRNA, intricately associated with processes such as cell proliferation, differentiation, and apoptosis and integral to the developmental intricacies of stromal follicles. The findings of this study provide a solid theoretical foundation for delving deeper into the realm of reproductive aging in Kazakh mares, presenting itself as a pivotal regulatory pathway in the context of horse ovarian development.
Unveiling rural energy pattern determinants: insights from forest-dwelling rural households in the Zagros Mountains, Iran
Authors: Bazgir Ahmad , Maleknia Rahim , Rahimian Mehdi
Published: January 2024

Introduction

This study examines the determinants of energy sources among rural households in the forested regions of the Zagros Mountains in Iran.

Methods

The research focuses on 157 forest villages, categorized into three groups based on their access to different fuel sources: wood, kerosene and liquefied petroleum gas (LPG), and natural gas (NG). A survey was conducted among 346 rural households using a multi-stage stratified random sampling approach and a questionnaire.

Results

The findings reveal that firewood plays a significant role in household energy patterns, particularly in remote areas. Income is identified as a crucial determinant of energy structure, but non-income socio-economic factors also strongly influence energy patterns. Moreover, commercial energy sources exhibit higher energy efficiency, resulting in reduced annual energy consumption compared to the use of firewood as the primary fuel.

Discussion

The study emphasizes the urgent need to address the reliance on low-efficiency energy sources to mitigate deforestation risks. In countries like Iran, where forest resources are limited, transitioning from firewood to higher efficiency fuels becomes imperative for forest conservation and sustainability. The implications of this research underscore the importance of considering both economic and social dimensions in energy planning and interventions, promoting accessible and affordable alternatives to traditional biomass fuels, and designing targeted policies to encourage the adoption of cleaner and more efficient energy sources in rural areas.
CAR Immunotherapy for the treatment of infectious diseases: a systematic review
Authors: Morte-Romea Elena , Pesini Cecilia , Pellejero-Sagastizábal Galadriel , Letona-Giménez Santiago , Martínez-Lostao Luis , Aranda Silvia Loscos , Toyas Carla , Redrado Sergio , Dolader-Ballesteros Elena , Arias Maykel , Galvez Eva M. , Sanz-Pamplona Rebeca , Pardo Julián , Paño-Pardo Jose Ramón , Ramírez-Labrada Ariel
Published: January 2024

Immunotherapy treatments aim to modulate the host’s immune response to either mitigate it in inflammatory/autoimmune disease or enhance it against infection or cancer. Among different immunotherapies reaching clinical application during the last years, chimeric antigen receptor (CAR) immunotherapy has emerged as an effective treatment for cancer where different CAR T cells have already been approved. Yet their use against infectious diseases is an area still relatively poorly explored, albeit with tremendous potential for research and clinical application. Infectious diseases represent a global health challenge, with the escalating threat of antimicrobial resistance underscoring the need for alternative therapeutic approaches. This review aims to systematically evaluate the current applications of CAR immunotherapy in infectious diseases and discuss its potential for future applications. Notably, CAR cell therapies, initially developed for cancer treatment, are gaining recognition as potential remedies for infectious diseases. The review sheds light on significant progress in CAR T cell therapy directed at viral and opportunistic fungal infections.
Immunomodulation in non-alcoholic fatty liver disease: exploring mechanisms and applications
Authors: Guo Ziwei , Wu Qinjuan , Xie Pengfei , Wang Jiuchong , Lv Wenliang
Published: January 2024

Non-alcoholic fatty liver disease (NAFLD) exhibits increased lipid enrichment in hepatocytes. The spectrum of this disease includes stages such as nonalcoholic simple fatty liver (NAFL), nonalcoholic steatohepatitis (NASH), and liver fibrosis. Changes in lifestyle behaviors have been a major factor contributing to the increased cases of NAFLD patients globally. Therefore, it is imperative to explore the pathogenesis of NAFLD, identify therapeutic targets, and develop new strategies to improve the clinical management of the disease. Immunoregulation is a strategy through which the organism recognizes and eliminates antigenic foreign bodies to maintain physiological homeostasis. In this process, multiple factors, including immune cells, signaling molecules, and cytokines, play a role in governing the evolution of NAFLD. This review seeks to encapsulate the advancements in research regarding immune regulation in NAFLD, spanning from underlying mechanisms to practical applications.
Gut dysbiosis in patients with chronic pain: a systematic review and meta-analysis
Authors: Goudman Lisa , Demuyser Thomas , Pilitsis Julie G. , Billot Maxime , Roulaud Manuel , Rigoard Philippe , Moens Maarten
Published: January 2024

Introduction

Recent evidence supports the contribution of gut microbiota dysbiosis to the pathophysiology of rheumatic diseases, neuropathic pain, and neurodegenerative disorders. The bidirectional gut-brain communication network and the occurrence of chronic pain both involve contributions of the autonomic nervous system and the hypothalamic pituitary adrenal axis. Nevertheless, the current understanding of the association between gut microbiota and chronic pain is still not clear. Therefore, the aim of this study is to systematically evaluate the existing knowledge about gut microbiota alterations in chronic pain conditions.

Methods

Four databases were consulted for this systematic literature review: PubMed, Web of Science, Scopus, and Embase. The Newcastle-Ottawa Scale was used to assess the risk of bias. The study protocol was prospectively registered at the International prospective register of systematic reviews (PROSPERO, CRD42023430115). Alpha-diversity, β-diversity, and relative abundance at different taxonomic levels were summarized qualitatively, and quantitatively if possible.

Results

The initial database search identified a total of 3544 unique studies, of which 21 studies were eventually included in the systematic review and 11 in the meta-analysis. Decreases in alpha-diversity were revealed in chronic pain patients compared to controls for several metrics: observed species (SMD= -0.201, 95% CI from -0.04 to -0.36, p=0.01), Shannon index (SMD= -0.27, 95% CI from -0.11 to -0.43, p<0.001), and faith phylogenetic diversity (SMD -0.35, 95% CI from -0.08 to -0.61, p=0.01). Inconsistent results were revealed for beta-diversity. A decrease in the relative abundance of the Lachnospiraceae family, genus Faecalibacterium and Roseburia, and species of Faecalibacterium prausnitzii and Odoribacter splanchnicus, as well as an increase in Eggerthella spp., was revealed in chronic pain patients compared to controls.

Discussion

Indications for gut microbiota dysbiosis were revealed in chronic pain patients, with non-specific disease alterations of microbes.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42023430115.
Composition of the sinking particle flux in a hot spot of dinitrogen fixation revealed through polyacrylamide gel traps
Authors: Ababou Fatima-Ezzahra , Le Moigne Frédéric A. C. , Cornet-Barthaux Véronique , Taillandier Vincent , Bonnet Sophie
Published: January 2024

Diazotrophs regulate marine productivity in the oligotrophic ocean by alleviating nitrogen limitation, contributing to particulate organic carbon (POC) export to the deep ocean. Yet, the characterization of particles composing the sinking POC flux has never been explored in such ecosystems. Moreover, the contribution of the direct gravitational export of diazotrophs to the overall flux is seldom assessed. Here we explore the composition of the sinking POC flux in a hot spot of N2 fixation (the western sub-tropical South Pacific) using polyacrylamide gel-filled traps deployed at two stations (S05M and S10M) and three depths (170 m, 270 m, 1000 m) during the TONGA expedition (November-December 2019). Image analyses of particles collected in the gels was used to classify them into 5 categories (fecal aggregates, phytodetrital aggregates, mixed aggregates, cylindrical fecal pellets, and zooplankton carcasses). Fecal aggregates were the most abundant at both stations and all depths and dominated the flux (average of 56 ± 28% of the POC flux), followed by zooplankton carcasses (24 ± 19%), cylindrical fecal pellets (15 ± 14%) and mixed aggregates (5 ± 4%), whereas phytodetrital aggregates contributed less (<1%). Since N isotope budgets show that export is mainly supported by diazotrophy at these stations, these results suggest that the diazotroph-derived N has been efficiently transferred to the foodweb up to zooplankton and fecal pellets before being exported, pleading for an indirect export of diazotrophy. However, random confocal microscopy examination performed on sinking particles revealed that diazotrophs were present in several categories of exported particles, suggesting that diazotrophs are also directly exported, with a potential contribution to overall POC fluxes increasing with depth. Our results provide the first characterization of particle categories composing the sinking flux and their contribution to the overall flux in a hot spot of N2 fixation.
Kelp dynamics and environmental drivers in the southern Salish Sea, British Columbia, Canada
Authors: Mora-Soto Alejandra , Schroeder Sarah , Gendall Lianna , Wachmann Alena , Narayan Gita R. , Read Silven , Pearsall Isobel , Rubidge Emily , Lessard Joanne , Martell Kathryn , Wills Peter , Costa Maycira
Published: January 2024

The impacts of local-scale temperatures and winds on bull kelp (Nereocystis luetkeana) vary along a coastal gradient, while also being influenced by corresponding global-scale oceanic conditions. Around Vancouver Island and the Gulf Islands, BC, Canada, bull kelp floating canopies were mapped using high-resolution imagery from 2005 to 2022, whereas the largest kelp bed of the area was mapped with medium-resolution imagery spanning from 1972 to 2022. In order to understand spatial patterns of kelp resilience, the abiotic characteristics were used to organize the coastline into four clusters, ranging from the coldest and most exposed coast to a more sheltered and warmer location. Additionally, local-scale sea surface temperatures, winds, and marine heatwaves were categorized by global-scale temporal conditions defined by the positive/negative oceanic oscillations of the Oceanic Niño Index (ONI) and Pacific Decadal Oscillation (PDO). Comparing spatial and temporal categories, we observed that years with positive ONI and PDO, in particular the 2014–2019 period, concentrated most of the marine heatwaves and the spring temperature peaks. However, there are some indications of an underlying long-term trend. During the period 2020–2022, when ONI and PDO were negative, summer temperatures kept increasing and wind displayed a higher frequency of extreme events. Mapped kelp showed different trends to these stressors: the coldest and most exposed area showed a constant presence of kelp during the entire period, even dating back to 1972. Warmer and semi-sheltered coasts increased in kelp percentage cover after the positive ONI+PDO period of 2014–2019, and the coasts facing the Strait of Georgia displayed a lower kelp percentage cover than the other clusters. In summary, bull kelp was resilient in the study area, but for different reasons: colder and more exposed coasts had the most favorable conditions for kelp, but warmer and more sheltered coastal kelp beds may have benefited from wind-wave forcing.
Areal bone mineral density, trabecular bone score and 3D-DXA analysis of proximal femur in psoriatic disease
Authors: Toussirot Eric , Winzenrieth Renaud , Aubin François , Wendling Daniel , Vauchy Charline , Desmarets Maxime
Published: January 2024

Objectives

To evaluate bone mineral density (BMD) and bone quality, with assessment of the cortical and trabecular compartments, in patients with psoriasis (PsO) alone or with psoriatic arthritis (PsA).

Methods

Patients with PsA and patients with PsO alone were evaluated and compared to control subjects matched for age, sex and body mass index category. Areal BMD (aBMD) was determined for the lumbar spine, femoral neck, total hip and total body using dual-energy X-ray absorptiometry (DXA). Bone quality was evaluated by using trabecular bone score (TBS) at the lumbar spine, and by 3D DXA-based analysis (3D Shaper) for the proximal femur.

Results

One hundred ninety-six subjects including 52 patients with PsA and 52 patients with PsO and their respective paired controls were analyzed. Patients with PsA had comparable aBMD, TBS and 3D DXA analysis parameters compared to their paired controls. After adjustment for confounders, patients with PsO alone were characterized by a higher aBMD at the left femur and higher cortical 3D DXA derived parameters (total hip cortical surface BMD and total hip cortical thickness) than their paired controls. TBS was decreased in PsO compared to their controls.

Conclusion

Patients with PsA had normal bone mass and bone quality parameters. Patients with PsO were characterized by higher femoral neck bone density by DXA and cortical parameters by 3D DXA-based analysis, supporting no increased risk for hip fracture. Conversely, bone texture by TBS assessment was decreased in patients with PsO, which may be associated with impaired vertebral bone resistance.
Harnessing fungal bio-electricity: a promising path to a cleaner environment
Authors: Umar Aisha , Mubeen Mustansar , Ali Iftikhar , Iftikhar Yasir , Sohail Muhammad Aamir , Sajid Ashara , Kumar Ajay , Solanki Manoj Kumar , Kumar Divvela Praveen , Zhou Lei
Published: January 2024

Integrating fungi into fuel cell systems presents a promising opportunity to address environmental pollution while simultaneously generating energy. This review explores the innovative concept of constructing wetlands as fuel cells for pollutant degradation, offering a practical and eco-friendly solution to pollution challenges. Fungi possess unique capabilities in producing power, fuel, and electricity through metabolic processes, drawing significant interest for applications in remediation and degradation. Limited data exist on fungi’s ability to generate electricity during catalytic reactions involving various enzymes, especially while remediating pollutants. Certain species, such as Trametes versicolor, Ganoderma lucidum, Galactomyces reessii, Aspergillus spp., Kluyveromyce smarxianus, and Hansenula anomala, have been reported to generate electricity at 1200 mW/m3, 207 mW/m2, 1,163 mW/m3, 438 mW/m3, 850,000 mW/m3, and 2,900 mW/m3, respectively. Despite the eco-friendly potential compared to conventional methods, fungi’s role remains largely unexplored. This review delves into fungi’s exceptional potential as fuel cell catalysts, serving as anodic or cathodic agents to mitigate land, air, and water pollutants while simultaneously producing fuel and power. Applications cover a wide range of tasks, and the innovative concept of wetlands designed as fuel cells for pollutant degradation is discussed. Cost-effectiveness may vary depending on specific contexts and applications. Fungal fuel cells (FFCs) offer a versatile and innovative solution to global challenges, addressing the increasing demand for alternative bioenergy production amid population growth and expanding industrial activities. The mechanistic approach of fungal enzymes via microbial combinations and electrochemical fungal systems facilitates the oxidation of organic substrates, oxygen reduction, and ion exchange membrane orchestration of essential reactions. Fungal laccase plays a crucial role in pollutant removal and monitoring environmental contaminants. Fungal consortiums show remarkable potential in fine-tuning FFC performance, impacting both power generation and pollutant degradation. Beyond energy generation, fungal cells effectively remove pollutants. Overall, FFCs present a promising avenue to address energy needs and mitigate pollutants simultaneously.
Enhancing hazardous material vehicle detection with advanced feature enhancement modules using HMV-YOLO
Authors: Wang Ling , Liu Bushi , Shao Wei , Li Zhe , Chang Kailu , Zhu Wenjie
Published: January 2024

The transportation of hazardous chemicals on roadways has raised significant safety concerns. Incidents involving these substances often lead to severe and devastating consequences. Consequently, there is a pressing need for real-time detection systems tailored for hazardous material vehicles. However, existing detection methods face challenges in accurately identifying smaller targets and achieving high precision. This paper introduces a novel solution, HMV-YOLO, an enhancement of the YOLOv7-tiny model designed to address these challenges. Within this model, two innovative modules, CBSG and G-ELAN, are introduced. The CBSG module's mathematical model incorporates components such as Convolution (Conv2d), Batch Normalization (BN), SiLU activation, and Global Response Normalization (GRN) to mitigate feature collapse issues and enhance neuron activity. The G-ELAN module, building upon CBSG, further advances feature fusion. Experimental results showcase the superior performance of the enhanced model compared to the original one across various evaluation metrics. This advancement shows great promise for practical applications, particularly in the context of real-time monitoring systems for hazardous material vehicles.
Problems in management of medication overuse headache in transgender and gender non-conforming populations
Authors: Martinez Cameron I. , Liktor-Busa Erika , Largent-Milnes Tally M.
Published: January 2024

Primary headache disorders, such as migraine, account for a significant portion of disability rates worldwide, yet patients still struggle to receive the adequate medical and emotional support necessary to improve health outcomes. Insufficient pain management through either impractical pharmaceutical treatments or absent emotional support networks can worsen physical and mental health outcomes since comorbidities commonly associated with headache include hypertension, diabetes, depression, and anxiety. A lack of awareness on headache pathology and its observable severity can lead to pain-related prejudice that destroys beneficial aspects of patient self-advocacy and self-efficacy, thus potentially discouraging the use of healthcare services in favor of maladaptive coping skills. Acute treatments for primary headache disorders include non-steroidal anti-inflammatory drugs (i.e., aspirin, ibuprofen), triptans (i.e., sumatriptan), and opioids; however, continuous use of these pain-relieving agents can generate a secondary headache known as medication overuse headache (MOH). Recent work highlighting the overlap of morphological and functional brain changes in MOH and substance use disorder (SUD) suggests that insufficient pain management encourages analgesic misuse. The LGBTQ+ community—specifically transgender and gender non-conforming persons—struggles with high rates of mental illness and substance abuse. Since gender-affirming sex hormone therapy influences migraine progression, transgender and gender non-conforming (trans*) patients on hormone therapy have a higher risk for worsening migraine symptoms. However, trans* patients are less likely to have access to appropriate pain management techniques, thus preventing positive health outcomes for this vulnerable population.
Risk stratification for early mortality in newly diagnosed acute promyelocytic leukemia: a multicenter, non-selected, retrospective cohort study
Authors: Kim Suhyeon , Jung Jiye , Ahn Seo-Yeon , Kim Mihee , Jeon So Yeon , Lee Chang-Hoon , Kim Dae Sik , Lee Se Ryeon , Sung Hwa Jung , Choi Chul Won , Kim Byung-Soo , Kim Hyeoung-Joon , Kwak Jae-Yong , Park Yong , Ahn Jae-Sook , Yhim Ho-Young
Published: January 2024

Introduction

Despite the current effective treatments for acute promyelocytic leukemia (APL), early mortality (EM), defined as death within 30 days of presentation, is a major hurdle to long-term survival.

Methods

We performed a multicenter retrospective study to evaluate the incidence and clinical characteristics of EM in patients with newly diagnosed APL and to develop a risk stratification model to predict EM.

Results

We identified 313 eligible patients diagnosed between 2000 and 2021 from five academic hospitals. The median age was 50 years (range 19-94), and 250 (79.9%) patients were <65 years. Most patients (n=274, 87.5%) received their first dose of all-trans retinoic acid (ATRA) within 24 hours of presentation. EM occurred in 41 patients, with a cumulative incidence of 13.1%. The most common cause of EM was intracranial hemorrhage (n=22, 53.6%), and most EMs (31/41, 75.6%) occurred within the first seven days of APL presentation. In a multivariable analysis, we identified three independent factors predicting EM: age ≥65 years (HR, 2.56), white blood cell count ≥8.0 x 109/L (HR, 3.30), and ATRA administration >24 hours of presentation (HR, 2.95). Based on these factors, patients were stratified into three categories with a significantly increasing risk of EM: 4.1% for low risk (54.3%; no risk factors; HR 1), 18.5% for intermediate risk (34.5%; 1 factor; HR 4.81), and 40.5% for high risk (11.2%; 2-3 factors; HR 13.16).

Discussion

The risk of EM is still not negligible in this era of ATRA-based therapies. Our risk model serves as a clinically useful tool to identify high-risk patients for EM who may be candidates for novel treatments and aggressive supportive strategies.
Ovarian steroid cell tumors: what do we know so far?
Authors: Wei Christina H. , Fadare Oluwole
Published: January 2024

Steroid cell tumors (SCT) of the ovary are rare, which has limited advances in the understanding of this enigmatic neoplasm. In this review, we summarize currently known clinicopathologic information on SCT. SCT are frequently hormonally active, leading to elevated serum and/or urine levels of androgenic hormones or their metabolites, and associated symptomatology, including virilization. The reported age at diagnosis is broad and has ranged from as young as 1 year old to 93 years old, although most patients were between ages 20 and 40 years. Most tumors are stage I and unilateral. The tumors are usually well circumscribed with a solid or solid to cystic cut surface. The tumors in one series reportedly ranged in size from 1.2 to 45 cm (average 8.4 cm). MRI is a useful imaging modality, typically showing a well delineated mass with contrast enhancement and lipid content on T2 and T1 weighted images, respectively. Microscopically, SCT display polygonal to epithelioid cells with abundant eosinophilic to vacuolated/clear cytoplasm and display an immunoprofile that is consistent with sex cord-stromal differentiation. Most cases are benign, without any recurrences after primary resection, but a subset – probably less than 20% of cases –are clinically malignant. Pathologic criteria that can specifically predict patient outcomes remain elusive, although features that correlate with adverse outcomes have been proposed based on retrospective studies. The molecular characteristics of SCTs are similarly under characterized, although there is some evidence of an enrichment for hypoxia-signaling gene mutations in SCT. In malignant SCT, the tumors generally show greater global genomic instability, copy number gains in oncogenes, and occasional BAP1 mutation. Future studies involving multi-institutional cohort and unbiased molecular profiling using whole exome/transcriptome sequencing are needed to help advance our molecular understanding of SCTs.
Research progress on the physiological response and molecular mechanism of cold response in plants
Authors: Wang Yong , Wang Jin , Sarwar Rehman , Zhang Wei , Geng Rui , Zhu Ke-Ming , Tan Xiao-Li
Published: January 2024

Low temperature is a critical environmental stress factor that restricts crop growth and geographical distribution, significantly impacting crop quality and yield. When plants are exposed to low temperatures, a series of changes occur in their external morphology and internal physiological and biochemical metabolism. This article comprehensively reviews the alterations and regulatory mechanisms of physiological and biochemical indices, such as membrane system stability, redox system, fatty acid content, photosynthesis, and osmoregulatory substances, in response to low-temperature stress in plants. Furthermore, we summarize recent research on signal transduction and regulatory pathways, phytohormones, epigenetic modifications, and other molecular mechanisms mediating the response to low temperatures in higher plants. In addition, we outline cultivation practices to improve plant cold resistance and highlight the cold-related genes used in molecular breeding. Last, we discuss future research directions, potential application prospects of plant cold resistance breeding, and recent significant breakthroughs in the research and application of cold resistance mechanisms.
Artificial intelligence and voting advice applications
Authors: Gemenis Kostas
Published: January 2024

The voter information tools collectively known as “Voting Advice Applications” (VAAs) have emerged as particularly popular tools in the realm of E-participation. Today, VAAs are integral parts of election campaigns in many countries around the world as they routinely engage millions of citizens, in addition to political actors and the media. This contribution assesses the integration of Artificial Intelligence (AI) in the design and dissemination of VAAs, considering normative, ethical, and methodological challenges. The study provides a comprehensive overview of AI applications in VAA development, from formulating questions to disseminating information, and concludes by highlighting areas where AI can serve as a valuable tool for enhancing the positive impact of VAAs on democratic processes.
SARS-CoV-2 Spike Protein Stimulates Macropinocytosis in Murine and Human Macrophages via PKC-NADPH Oxidase Signaling
Authors: Ahn WonMo , Burnett Faith N. , Pandey Ajay , Ghoshal Pushpankur , Singla Bhupesh , Simon Abigayle B. , Derella Cassandra C. , A. Addo Stephen , Harris Ryan A. , Lucas Rudolf , Csányi Gábor
Published: January 2024

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While recent studies have demonstrated that SARS-CoV-2 may enter kidney and colon epithelial cells by inducing receptor-independent macropinocytosis, it remains unknown whether this process also occurs in cell types directly relevant to SARS-CoV-2-associated lung pneumonia, such as alveolar epithelial cells and macrophages. The goal of our study was to investigate the ability of SARS-CoV-2 spike protein subunits to stimulate macropinocytosis in human alveolar epithelial cells and primary human and murine macrophages. Flow cytometry analysis of fluid-phase marker internalization demonstrated that SARS-CoV-2 spike protein subunits S1, the receptor-binding domain (RBD) of S1, and S2 stimulate macropinocytosis in both human and murine macrophages in an angiotensin-converting enzyme 2 (ACE2)-independent manner. Pharmacological and genetic inhibition of macropinocytosis substantially decreased spike-protein-induced fluid-phase marker internalization in macrophages both in vitro and in vivo. High-resolution scanning electron microscopy (SEM) imaging confirmed that spike protein subunits promote the formation of membrane ruffles on the dorsal surface of macrophages. Mechanistic studies demonstrated that SARS-CoV-2 spike protein stimulated macropinocytosis via NADPH oxidase 2 (Nox2)-derived reactive oxygen species (ROS) generation. In addition, inhibition of protein kinase C (PKC) and phosphoinositide 3-kinase (PI3K) in macrophages blocked SARS-CoV-2 spike-protein-induced macropinocytosis. To our knowledge, these results demonstrate for the first time that SARS-CoV-2 spike protein subunits stimulate macropinocytosis in macrophages. These results may contribute to a better understanding of SARS-CoV-2 infection and COVID-19 pathogenesis.
Fuzzy Cognitive Map Applications in Medicine over the Last Two Decades: A Review Study
Authors: Apostolopoulos Ioannis D. , Papandrianos Nikolaos I. , Papathanasiou Nikolaos D. , Papageorgiou Elpiniki I.
Published: January 2024

Fuzzy Cognitive Maps (FCMs) have become an invaluable tool for healthcare providers because they can capture intricate associations among variables and generate precise predictions. FCMs have demonstrated their utility in diverse medical applications, from disease diagnosis to treatment planning and prognosis prediction. Their ability to model complex relationships between symptoms, biomarkers, risk factors, and treatments has enabled healthcare providers to make informed decisions, leading to better patient outcomes. This review article provides a thorough synopsis of using FCMs within the medical domain. A systematic examination of pertinent literature spanning the last two decades forms the basis of this overview, specifically delineating the diverse applications of FCMs in medical realms, including decision-making, diagnosis, prognosis, treatment optimisation, risk assessment, and pharmacovigilance. The limitations inherent in FCMs are also scrutinised, and avenues for potential future research and application are explored.
Association of Four Interleukin-8 Polymorphisms (−251 A>T, +781 C>T, +1633 C>T, +2767 A>T) with Ovarian Cancer Risk: Focus on Menopausal Status and Endometriosis-Related Subtypes
Authors: Watrowski Rafał , Schuster Eva , Hofstetter Gerda , Fischer Michael B. , Mahner Sven , Van Gorp Toon , Polterauer Stefan , Zeillinger Robert , Obermayr Eva
Published: January 2024

Interleukin-8 (IL-8) is involved in the regulation of inflammatory processes and carcinogenesis. Single-nucleotide polymorphisms (SNPs) within the IL-8 gene have been shown to alter the risks of lung, gastric, or hepatocellular carcinomas. To date, only one study examined the role of IL-8 SNPs in ovarian cancer (OC), suggesting an association between two IL-8 SNPs and OC risk. In this study, we investigated four common IL-8 SNPs, rs4073 (−251 A>T), rs2227306 (+781 C>T), rs2227543 (+1633 C>T), and rs1126647 (+2767 A>T), using the restriction fragment length polymorphism (PCR-RFLP) technique. Our study included a cohort of 413 women of Central European descent, consisting of 200 OC patients and 213 healthy controls. The most common (73.5%) histological type was high-grade serous OC (HGSOC), whereas 28/200 (14%) patients had endometriosis-related (clear cell or endometrioid) OC subtypes (EROC). In postmenopausal women, three of the four investigated SNPs, rs4073 (−251 A>T), rs2227306 (+781 C>T), and rs2227543 (+1633 C>T), were associated with OC risk. Furthermore, we are the first to report a significant relationship between the T allele or TT genotype of SNP rs1126647 (+2767 A>T) and the EROC subtype (p = 0.02 in the co-dominant model). The TT homozygotes were found more than twice as often in EROC compared to other OC subtypes (39% vs. 19%, p = 0.015). None of the examined SNPs appeared to influence OC risk in premenopausal women, nor were they associated with the aggressive HGSOC subtype or the stage of disease at the initial diagnosis.
The FLNC Ala1186Val Variant Linked to Cytoplasmic Body Myopathy and Cardiomyopathy Causes Protein Instability
Authors: Onnée Marion , Bénézit Audrey , Bastu Sultan , Nadaj-Pakleza Aleksandra , Lannes Béatrice , Ader Flavie , Thèze Corinne , Cintas Pascal , Cances Claude , Carlier Robert-Yves , Metay Corinne , Cossée Mireille , Malfatti Edoardo
Published: January 2024

Filamin C-related disorders include myopathies and cardiomyopathies linked to variants in the FLNC gene. Filamin C belongs to a family of actin-binding proteins involved in sarcomere stability. This study investigates the pathogenic impact of the FLNC c.3557C > T (p.Ala1186Val) pathogenic variant associated with an early-onset cytoplasmic body myopathy and cardiomyopathy in three unrelated patients. We performed clinical imaging and myopathologic and genetic characterization of three patients with an early-onset myopathy and cardiomyopathy. Bioinformatics analysis, variant interpretation, and protein structure analysis were performed to validate and assess the effects of the filamin C variant. All patients presented with a homogeneous clinical phenotype marked by a severe contractural myopathy, leading to loss of gait. There was prominent respiratory involvement and restrictive or hypertrophic cardiomyopathies. The Ala1186Val variant is located in the interstrand loop involved in intradomain stabilization and/or interdomain interactions with neighbor Ig-like domains. 3D modeling highlights local structural changes involving nearby residues and probably impacts the protein stability, causing protein aggregation in the form of cytoplasmic bodies. Myopathologic studies have disclosed the prominent aggregation and upregulation of the aggrephagy-associated proteins LC3B and p62. As a whole, the Ala1186Val variant in the FLNC gene provokes a severe myopathy with contractures, respiratory involvement, and cardiomyopathy due to protein aggregation in patients’ muscles.
Path for Room-Temperature Superconductivity in Q-Carbon-Related Materials
Authors: Narayan Jagdish
Published: January 2024

We present the atomic structures and nonequilibrium synthesis of a new class of materials, where the basic structural unit is a diamond tetrahedron. When units of one, two, and three tetrahedra are randomly packed, we create distinct phases of amorphous Q-carbon. Four tetrahedra in two adjacent layers lead to crystalline diamond lattice, which has four missing tetrahedra alternately. When these four missing tetrahedra are filled, we create subunit cell of crystalline Q-diamond. Theoretical calculations show that the superconducting transition temperature (Tc) in 50 atomic % B-doped Q-diamond can reach room temperature at ambient pressures. This is consistent with our earlier results using low-loss EELS measurements in 50 atomic % B-doped Q-carbon, which had mostly amorphous QB3 phase mixed with some crystalline Q-diamond phase. These EELS results showed that the Tc for these samples was between 90 K and 300 K. Theoretical calculations of density of states, Eliashberg function, electron–phonon interaction parameter, and root-mean-square and logarithmic average of frequency in crystalline Q-diamond show Tc in the range of 268 K to 300 K, which is in complete agreement with our EELS results in QB3.
The Effects of a Digital Game Simulator versus a Traditional Intervention on Paramedics’ Neonatal Resuscitation Performance
Authors: Cutumisu Maria , Schmölzer Georg M.
Published: January 2024

Neonatal resuscitation is a skill set that comprises procedures, assessment, decision-making, communication, and teamwork. It is used in an emergency situation in the delivery room with the aim of supporting newborn infants who are not able to begin breathing on their own. Thus, healthcare providers need to refresh their neonatal resuscitation skills periodically, according to the Neonatal Resuscitation Program, to ensure that they can react quickly and effectively in emergency situations. The RETAIN digital game simulator was designed to enable healthcare providers to practice their neonatal resuscitation skills. To evaluate the effectiveness of this game in a laboratory setting, a randomized control trial sampled 42 paramedics who completed a pre-test, were randomly assigned to watch a traditional lecture video on the neonatal resuscitation procedure or to play a novel digital game simulation on the same topic, and then completed a following test. A two-way mixed ANOVA revealed a statistically significant improvement in paramedics’ neonatal resuscitation performance over time, which did not differ between conditions. Thus, digital games can provide an enjoyable alternative to traditional practices in refreshing neonatal resuscitation knowledge.