bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of resveratrol to supplement oral nifedipine treatment in pregnancy-induced preeclampsia
Authors: Ding Jian , Kang Yan , Fan Yuqin , Chen Qi
Published: October 2017

Objective

Preeclampsia (PE) is a complication affecting pregnant women worldwide, which usually manifests as severe maternal hypertension. Resveratrol (RESV), a naturally existing polyphenol, is known to exhibit beneficial effects in cardiovascular disease including hypertension. We evaluated the outcome of treatment combining oral nifedipine (NIFE) and RESV against PE.

Design and methods

Using a randomized group assignment, 400 PE patients were enrolled and received oral treatments of either NIFE + RESV or NIFE + placebo. Primary endpoints were defined as time to control blood pressure and time before a new hypertensive crisis. Secondary endpoints were defined as the number of doses needed to control blood pressure, maternal and neonatal adverse effects.

Results

Compared with the NIFE + placebo group, the time needed to control blood pressure was significantly reduced in NIFE + RESV group, while time before a new hypertensive crisis was greatly delayed in NIFE + RESV group. The number of treatment doses needed to control blood pressure was also categorically lower in NIFE + RESV group. No differences in maternal or neonatal adverse effects were observed between the two treatment groups.

Conclusion

Our data support the potential of RESV as a safe and effective adjuvant of oral NIFE to attenuate hypertensive symptoms among PE patients.
Ativismo Virtual na Pós-Modernidade das Experiências e Afetos
Authors: Almeida Mariana Leite de
Published: October 2017

Paradigmas dicotômicos ainda presentes na pós-modernidade nos permitem observar o quanto ainda estamos ligados à modernidade mais racionalista. Vivemos tempos coletivos, porém individualistas, vivemos ao mesmo tempo libertos e aprisionados, informados, mas constantemente passivos. Em diversos momentos vivemos, ainda, sem reconhecer o outro enquanto legítimo. Essa falta de reconhecimento do outro, muitas vezes, faz com que nos tornemos menos comprometidos socialmente e não demonstremos, no cotidiano social, a coletividade constantemente vivida na internet. As redes sociais nos mantêm fortemente ligados através de laços sensíveis, repletos de emoções e afetos. Essas relações colaboram na construção de uma ideia de que aprendizados se fazem possíveis graças ao que é experienciado com o mundo e, principalmentecom o outro. Nesse contexto, o ativismo virtual se faz cada vez mais presente e necessário em nossas vidas, para podermos pensar criticamente sobre nossas experiências e sobre nossas vivências coletivas, buscando assim construir lugares melhores para se viver socialmente.
O imaginário do design: ensaio filosófico sobre os discursos do design
Authors: Beccari Marcos Namba
Published: October 2017

Este artigo apresenta uma reflexão filosófica a respeito das proposições que engendram os discursos paradigmáticos do design. Após uma breve contextualização do discurso funcionalista, discorro sobre algumas distinções (natureza x artifício, design x arte) que servem como princípios de uma mesma campanha que busca pleitear “seriedade” ao design. Por fim, proponho que a dimensão estética do design oferece uma imagem do que somos socialmente, como compreensão sensível das mediações simbólicas que nos perfazem. O objetivo geral, portanto, é problematizar o imaginário do design, contrapondo a imagem que os designers fazem de si mesmos à amplitude que o design assume enquanto modo de ser contemporâneo.
Numerical simulation of cascaded dam-break flow in downstream reservoir
Authors: Luo Jing , Xu Weilin , Tian Zhong , Chen Huayong
Published: October 2017

The evolution of dam-break floods in downstream and cascade reservoirs is an extremely complicated process. Based on the three-dimensional Navier–Stokes equation and smoothed particle hydrodynamics theory, the complicated dam-break flood flow was simulated for such cases. The evolution of the dam-break flood in the downstream reservoir and cascade reservoirs and its impact on the downstream dam were obtained, and the results were found to agree well with experimental results. According to force analysis, the dam-break flood effect can be divided into a high-speed impact area and a pressure-transmission area. The former can cause damage due to instantaneous impact on the dam and the latter can cause damage arising from oscillation fatigue. These conclusions have value in water resources and hydropower engineering for preventing the outburst of a cascade reservoir group.
Precision Transplant Medicine: Biomarkers to the Rescue
Authors: Naesens Maarten , Anglicheau Dany
Published: October 2017

The concept that individuals with the same disease and a similar clinical presentation may have very different outcomes and need very different therapies is not novel. With the development of many innovative tools derived from the omics technologies, transplant medicine is slowly entering the era of precision medicine. Biomarkers are the cornerstone of precision medicine, which aims to integrate biomarkers with traditional clinical information and tailor medical care to achieve the best outcome for an individual patient. Here, we discuss the basic concepts of precision medicine and biomarkers, with a specific focus on progress in renal transplantation. We delineate the different types of biomarkers and provide a general assessment of the current applications and shortcomings of previously proposed biomarkers. We also outline the potential of precision medicine in transplantation. Moving toward precision medicine in the field of transplantation will require transplant physicians to embrace the increased complexity and expanded decision algorithms and therapeutic options that are associated with improved disease nosology.
Kidney Proximal Tubule Lipoapoptosis Is Regulated by Fatty Acid Transporter-2 (FATP2)
Authors: Khan Shenaz , Cabral Pablo D. , Schilling William P. , Schmidt Zachary W. , Uddin Asif N. , Gingras Amelia , Madhavan Sethu M. , Garvin Jeffrey L. , Schelling Jeffrey R.
Published: October 2017

Albuminuria and tubular atrophy are among the highest risks for CKD progression to ESRD. A parsimonious mechanism involves leakage of albumin-bound nonesterified fatty acids (NEFAs) across the damaged glomerular filtration barrier and subsequent reabsorption by the downstream proximal tubule, causing lipoapoptosis. We sought to identify the apical proximal tubule transporter that mediates NEFA uptake and cytotoxicity. We observed transporter-mediated uptake of fluorescently labeled NEFA in cultured proximal tubule cells and microperfused rat proximal tubules, with greater uptake from the apical surface than from the basolateral surface. Protein and mRNA expression analyses revealed that kidney proximal tubules express transmembrane fatty acid transporter-2 (FATP2), encoded by Slc27a2, but not the other candidate transporters CD36 and free fatty acid receptor 1. Kidney FATP2 localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane. Treatment of mice with lipidated albumin to induce proteinuria caused a decrease in the proportion of tubular epithelial cells and an increase in the proportion of interstitial space in kidneys from wild-type but not Slc27a2−

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−
mice. Ex vivo microperfusion and in vitro experiments with NEFA-bound albumin at concentrations that mimic apical proximal tubule exposure during glomerular injury revealed significantly reduced NEFA uptake and palmitate-induced apoptosis in microperfused Slc27a2−/−
proximal tubules and Slc27a2−/−
or FATP2 shRNA-treated proximal tubule cell lines compared with wild-type or scrambled oligonucleotide–treated cells, respectively. We conclude that FATP2 is a major apical proximal tubule NEFA transporter that regulates lipoapoptosis and may be an amenable target for the prevention of CKD progression.
Cardiovascular Effects of Renal Distal Tubule Deletion of the FGF Receptor 1 Gene
Authors: Han Xiaobin , Ross Jed , Kolumam Ganesh , Pi Min , Sonoda Junichiro , King Gwendalyn , Quarles L. Darryl
Published: October 2017

The bone-derived hormone fibroblast growth factor–23 (FGF-23) activates complexes composed of FGF receptors (FGFRs), including FGFR1, and α-Klotho in the kidney distal tubule (DT), leading to increased sodium retention and hypertension. However, the role of FGFR1 in regulating renal processes linked to hypertension is unclear. Here, we investigated the effects of selective FGFR1 loss in the DT. Conditional knockout (cKO) of FGFR1 in the DT (FGFR1DT-cKO
mice) resulted in left ventricular hypertrophy (LVH) and decreased kidney expression of α-Klotho in association with enhanced BP, decreased expression of angiotensin converting enzyme 2, and increased expression of the Na+-K+-2Cl− cotransporter. Notably, recombinant FGF-23 administration similarly decreased the kidney expression of α-Klotho and induced LVH in mice. Pharmacologic activation of FGFR1 with a monoclonal anti-FGFR1 antibody (R1MAb1) normalized BP and significantly attenuated LVH in the Hyp mouse model of excess FGF-23, but did not induce a response in FGFR1DT-cKO
mice. The hearts of FGFR1DT-cKO
mice showed increased expression of the transient receptor potential cation channel, subfamily C, member 6 (TRPC6), consistent with cardiac effects of soluble Klotho deficiency. Moreover, administration of recombinant soluble Klotho lowered BP in the Hyp mice. Thus, FGFR1 in the DT regulates systemic hemodynamic responses opposite to those predicted by the actions of FGF-23. These cardiovascular effects appear to be mediated by paracrine FGF control of kidney FGFR1 and subsequent regulation of soluble Klotho and TRPC6. FGFR1 in the kidney may provide a new molecular target for treating hypertension.
Current approaches and methods in plant domestication studies
Authors: Guerra García Azalea , Piñero Daniel
Published: October 2017

Background: The domestication process has left signatures in the genomes of domesticated species. Before the existence of molecular markers, only phenotypic traits could be used in domestication studies and breeding programs, but these approaches required long time and effort. In the last decades, the use of molecular markers dramatically increased, and the development of massive sequencing tools have enable to obtain thousands or even millions of molecular markers. This work focuses on domesticated plants and the main goal is to bring a general and an integrative perspective of the data, approaches and questions that can be answered using massive sequencing tools, compared to classic genetic data.
Results: The use of molecular markers in the last decades has increased the efficiency and accuracy of plant breeding, allowing to access information about domestication history and to identify genes affected by domestication. Some patterns have been identified: (1) genetic diversity reduction due to demographic bottlenecks and artificial selection; (2) frequently, mutations related with domestication syndrome preexisted at low frequency in natural populations; (3) accumulation of deleterious mutation; (4) gene flow between wild and cultivated populations. There are several approaches that can be used in massive sequencing tools: de novo genome sequencing, whole genome resequencing, reduction of genome complexity using restriction enzymes, transcriptome analysis and epigenetic studies.
Conclusions: Despite the progress made, enormous challenges still remain: storage of large databases; development of fast, accurate and high throughput strategies to phenotype; identification of paralogous genes in polyploid species; and the analysis of large and highly diverse genomes.
Micromorphological character expression of the hybrid Quercus × dysophylla and its parental species (Q. crassifolia and  Q. crassipes)
Authors: López-Caamal Alfredo , Ruiz-Amaro Luz del Carmen , Zepeda-Rodríguez Armando , Mussali-Galante Patricia , Tovar-Sánchez Efraín
Published: October 2017

Background: Hybridization leads to individuals with atypical phenotypes, leading to taxonomic confusion when hybridizing populations are studied. Micromorphological characters may enable taxonomists to discriminate between parental and hybrid categories due to their simple genetic inheritance.
Species study: Three oak taxa distributed in the montane regions of the Mexico were studied: Quercus crassifolia, Q. crassipes and their hybrid Q. × dysophylla.
Hypothesis: We describe the leaf micromorphological and macromorphological variation of these taxa. Specifically, we searched for a unique combination of micromorphological characters in hybrids. We hypothesize that spatial micromorphological variation will match the spatial pattern revealed by a previous genetic study.
Study sites: Two allopatric stands for each parental species and seven hybrid zones were studied. Individuals within each stand were identified as belonging to parental or hybrid categories following previous genetic analyses.
Methods: Stomata and trichome types for each taxa were determined through Scanning Electron Microscopy. Eight micromorphological characters for trichomes and stomata and four foliar macromorphological characters were measured.
Results: We found the presence of both multirradiate and simple stellate non-glandular trichomes as a diagnostic feature of Q. × dysophylla. Overall populations, Q. × dysophylla showed intermediate phenotypes in 70 % of morphological characters. However, hybrid phenotype exhibited geographical variation. Lastly, spatial hybrid’s phenotype variation did not correlate with the spatial genetic pattern previously identified.
Conclusions: The micromorphological features of Q. × dysophylla may enable taxonomists to accurately discriminate between this taxon and its parental species. Finally, we suggest that micromorphological expression of both parental species and hybrids may be influenced by environmental gradients and microclimates.
Polymorphism analysis in some Algerian Opuntia species using morphological and phenological UPOV descriptors
Authors: Hadjkouider Boubakr , Boutekrabt Ammar , Lallouche Bahia , Lamine Salim , Zoghlami Néjia
Published: October 2017

Background: In the present study, we have investigated the morphological variation in a set of five Opuntia species from the Algerian steppes using 49 UPOV descriptors.
Questions: which of the 49 descriptors that can be used as powerful estimators of the phenotypic diversity within Opuntia species? How is the morphological diversity patterned in Algerian Opuntia?
Species study/ Mathematical model: Opuntia ficus-indica, Opuntia amycleae, Opuntia streptacantha, Opuntia engelmannii, Opuntia robusta. Principal Components Analysis (PCA) and Hierarchical Cluster Analysis were used.
Study site: Four counties were studied located in the Algerian steppes. The present research was carried out during 2014.
Methods: 49 descriptors adopted by the International Union for the Protection of New Varieties of Plants (UPOV) were employed in the present research, where cladode, flower and fruit traits were used to determine the overall degree of polymorphism among 5 Opuntia species.
Results: Principal Component Analysis and Hierarchical Cluster Analysis indicated a consistent differentiation between all studied species. The relative magnitude of the first two PCA eigenvectors showed that 8 descriptors out of 49 were identified as the most important descriptors for the classification of the species. The dendrogram performed on the calculated Euclidean distances between all species pairs allowed the identification of 3 groups, unlike the PCA that identified 4 groups. The species Opuntia ficus-indica and Opuntia amycleae were identified as very close morphologically.
Conclusions: The present outcome represents a paramount step towards the fast selection of interesting species and for their best management and conservation.
Population size, sex-ratio and sexual dimorphism in Fuchsia parviflora (Onagraceae) an endemic dioecious shrub
Authors: Cuevas Eduardo , Pérez María Ángeles , Sevillano Lucero
Published: October 2017

Background: In small dioecious populations stochastic events may increase biased sex ratios decreasing the viability of populations. Moreover, pollinators may promote pollen limitation if sexual dimorphism related to pollinator attraction, such as flower size and number of flowers are present.
Questions: 1) In order to estimate the viability of natural populations of Fuchsia parviflora, population size, sex ratio and fruit production were estimated in natural populations. In addition, number of flowers and floral traits were compared between genders as a way to predict pollinator preferences to particular gender.
Studied species: Fuchsia parviflora is an endemic Mexican dioecious, animal-pollinated shrub.
Methods: In 2012 and 2013 population sizes and sex ratios were estimated in 12 populations in the states of Michoacan and Jalisco. In addition, the number of flowers and floral traits were estimated between genders.
Results: Population sizes varied widely from 14 to 550 individuals and an overall significant male- bias was found. Male frequency varied from 50 % to 78 % among populations and some male-biased populations were also the small populations. A significant negative correlation between population size and male frequency was found, but no relationship was found between population size and number of flowers or fruit production. Reproductive plant height varied among populations but not between genders within populations. Male plants had larger number of flowers but floral longevity did not differ between genders. Consistently in all evaluated populations, staminate flowers had longer floral tubes and wider corolla diameter than pistillate flowers.
Conclusions: In accordance to stochastic sex ratio fluctuations by small population sizes, it was expected to find populations with male and female-biased sex ratios. However, the absence of female-biased sex ratio suggests that male plants may have a higher survival chance but this hypothesis has to be tested. Although populations seem not to be seed-limited, some populations were cut during this study to plant avocado trees, suggesting the need to implement conservation efforts to preserve populations of F. parviflora.
The impact of the "Developmental Resource Stimulation Programme"(DRSP) on children with Down syndrome.
Authors: Russell Dorothy Charmaine , Van Heerden Rita , Van Vuuren Santie , Venter André , Joubert Gina
Published: October 2017

Background: This study investigated the impact of an intensive early-intervention programme, the Developmental Resource Stimulation Programme (DRSP), on Down syndrome (DS) children younger than 42 months in the South African context. The DRSP is a unique, child-parent specific, one-on-one, integrated developmental programme for children with Down syndrome from birth to 42 months. Aim: The aim of this study was to investigate the impact of the DRSP on Down syndrome children younger than 42 months in the South African context. Methodology: A non-randomised control group pre-test-post-test design was followed. The Bayley Scales of Infant and Toddler Development, 3rd edition was used. Thirty children with the general characteristics of DS, specifically Trisomy 21, were included in the study. There were two groups, namely the intervention group (16 participants) and the control group (14) which were studied over a period of six months. Results: This study showed that a specifically designed programme, with participation of a parent, has a positive impact on the development of the child with Down syndrome. Conclusion: Contrary to the existing literature, there were positive changes in the fine-motor development and language of the intervention group overall and in the gross-motor development of children older than 9-months with Down syndrome.Key words: early intervention programme, Down syndrome, younger than three years, developmental domains, occupational therapy
Time management guidelines for the intervention of a child with Down Syndrome using the "Developmental Resource Stimulation Programme" (DRSP).
Authors: Russell Dorothy
Published: October 2017

Background: The duration of an intervention session for an intensive early-intervention programme, the Developmental Resource Stimulation Programme (DRSP), for Down syndrome (DS) children younger than 42 months, was investigated as part of a larger research study. The DRSP is a unique, child-parent specific, one-on-one integrated developmental programme for children with Down syndrome from birth to 42 months.Aim: The aim of this programme was to determine the required number of sessions per activity and optimal session length that enables a child younger than 42 months with DS to master the DRSP activities.Methodology: A descriptive study design was used to organise and summarise data from the DRSP checklist score-sheets. Sixteen children with common characteristics of DS, specifically Trisomy 21, and their parents formed part of the investigation. Results: The research established a time management guideline including the specific duration and frequency of executing an occupational performance activity by a child with DS in order for the child to master the activity.Conclusion: The recommendation is that individual sessions should occur fortnightly to implement the DRSP for children with DS from birth, with the suggested length of a session being 12-15 minutes for children younger than 18 months and 15-40 minutes for children older than 18 months and should be undertaken over a six month period.Key words: intervention session duration, Down syndrome, early intervention programme, developmental domains, occupational therapy.
Minimally invasive surgical treatment of carcinoma of the papilla of Vater: A case report
Authors: Rašić Ismar , Kotjelnikov Branislav , Glavaš Sanjin , Šabanović Jusuf , Holjan Sandin
Published: October 2017

Ampullary cancer is a rare neoplasm of the pancreaticobiliary tract. Most of the ampullary neoplasms require surgical resection. Cephalic pancreaticoduodenectomy (CPD) has been performed laparoscopically at high-volume institutions worldwide. In this paper, we present a 63-year-old patient with painless jaundice, subjected to proximal endoscopy and biopsy of the lesion located in the papilla of Vater, which resulted in a diagnosis of the adenocarcinoma of the papilla of Vater. We performed laparoscopic CPD with appropriate lymphadenectomy. To the best of our knowledge, this complex and technically challenging surgical procedure is the first of this kind in our country and the region.
Relação entre nutrição de idosos e dentição: Revisão de Literatura
Authors: Moura Selma Maria Santos , Leite Carla Maria Carvalho , Rocha Ana Makrina Carlos , Costa Mayara de Araújo Bastos , Oliveira Ingrid Macedo , Cardoso Jéssica de Carvalho
Published: October 2017

Na atualidade, onde se observa uma maior longevidade da vida populacional, a saúde bucal dos idosos tem sido observada com maior atenção por influenciar o estado nutricional do indivíduo. Esta revisão de literatura foi realizada para compreender a relação existente entre a perda dentária ou uso de prótese e o estado nutricional de pessoas na terceira idade. Foram consultadas as bases de dados da CAPES, MEDLINE, LILACS, a biblioteca virtual SciELO e a base de dados populacionais do IBGE, sendo consideradas as publicações de 1993 a 2014. A população idosa, de uma forma geral, apresenta elevados índices de perdas dentárias e especificamente no Brasil, dados do Ministério da Saúde, 2004, mostram que cerca de 54,8% da população com idade entre 64 e 74 anos é totalmente desdentada, dos quais 18,7% não faz uso de nenhuma prótese dentária. Estudos mostram que a ausência de elementos dentários e o uso de próteses trituram de forma menos eficiente os alimentos tendo como conseqüência a depauperação orgânica com o aumento dos problemas relacionados à digestão e absorção dos alimentos. Dessa forma, a má absorção dos nutrientes gera um desequilíbrio nutricional com prejuízo para a realização e manutenção de processos orgânicos vitais, maior susceptibilidade a enfermidades e dificuldade na recuperação de doenças. A literatura reporta a necessidade da realização de mais estudos com o intuito de identificar problemas nutricionais relacionados à saúde bucal e a partir desse ponto, buscar soluções adequadas que permitam o estabelecimento de políticas públicas de atenção à saúde oral possibilitando uma melhor qualidade de vida da população como um todo.
Análise Histológica de Traqueia e Pulmão de Neonatos Caninos
Authors: Silva Andrezza Braga Soares , Cavalcante Maria Michele Araujo de Sousa , Barros Vitor Jampierre da Silva , Lopes Luana Oliveira , Fernandes Heliana de Barros , Fortes Eunice Anita de Moura , Ferraz Maíra Soares , Rizzo Marcia dos Santos , Júnior Airton Mendes Conde
Published: October 2017

A neotalologia é a ciência, dentro da medicina veterinária, que estuda o período pós-nascimento até o desenvolvimento de certas características de resistência, que para os caninos se dá até a segunda semana de vida. O neonato exige uma abordagem precisa dada às particularidades de sua fisiologia e imunologia extremamente imaturas. O estudo histológico elucida problemas de anormalidade funcional e morfológica, na medida em que proporciona uma análise microscópica e fidedigna. Objetivou-se analisar traqueia e pulmão de neonatos caninos por meio de técnicas de histologia básica. Foram utilizados cinco neonatos, que vieram a óbito pós-parto. Estes foram pesados, medidos e posteriormente dissecados. Procedeu-se a extração da traqueia, brônquios e do pulmão para a submissão destas amostras à rotina histológica. O tecido traqueal apresenta um epitélio pseudoestratificado estereociliado com células caliciformes, pouca quantidade de glândulas na lâmina própria e cartilagem hialina não completamente desenvolvida. Quanto ao tecido dos brônquios, podem-se observar as camadas bem definidas, epitélio pulmonar pseudoestratificado estereociliado com células caliciformes, lâmina própria com vários feixes de músculo liso e espessa camada de tecido conjuntivo vascularizado. Da mesma forma, as placas de cartilagem nos brônquios apresentam-se em desenvolvimento. Os bronquíolos também apresentam o epitélio pulmonar comum e lâmina própria também normal, cercados sem músculo liso. Os sacos alveolares apresentaram células pulmonares típicas. Os tecidos pulmonares de neonatos caninos apresentam-se ainda em estágio de desenvolvimento. É possível entender padrões de histogênese e morfogênese em neonatos caninos.
Desenvolvimento motor em crianças praticantes e não praticantes de atividades lúdicas aquáticas
Authors: Silva Lauana Priscila , Dias Leticia Regina , Caetano Mabile Taize B. F. , Martelli Anderson , Frezzato Renata Camargo , Delbim Lucas Rissette
Published: October 2017

Entende-se como desenvolvimento motor a junção das características biológicas, meio ambiente e necessidades da tarefa. Sabe-se que é um processo permanente, o qual se inicia na concepção e só cessa com a morte do indivíduo. A estimulação diária das crianças pode acelerar e/ou melhorar esse desenvolvimento e, assim, as atividades lúdicas aquáticas se enquadram nessa estimulação, pois seu principal objetivo é a adaptação ao meio liquido, através do uso de brincadeiras, respeitando sempre o desenvolvimento motor de cada fase. O objetivo deste estudo foi avaliar o impacto da prática de atividades lúdicas aquáticas no desenvolvimento neuropsicomotor (DNPM) de crianças entre 5 a 6 anos de idade. A amostra foi dividida em dois grupos, um de crianças praticantes e outro de crianças não praticantes de atividades lúdicas aquáticas. Ambos os grupos foram avaliados com a Escala de Desenvolvimento Motor (EDM) sendo observado que o desempenho motor de ambos os grupos encontram-se dentro da normalidade esperada para as idades. Porém, a escassez de estudos com essa população sugere a necessidade de novos trabalhos investigativos sobre o assunto.
Purificação e avaliação da interferência de BthMP (uma metaloprotease da peçonha de Bothrops moojeni) na coagulação sanguínea
Authors: Santos Manuela Andrade , Pando Luzia Aparecida , Rodrigues Veridiana de Melo , Castro Mariana de Souza , Gomes Mário Sérgio Rocha
Published: October 2017

Neste trabalho relatamos a purificação da metaloprotease BthMP, proveniente da peçonha da serpente Bothrops moojeni. Para a purificação desta protease, utilizaram-se os passos cromatográficos de troca iônica (DEAE-Sepharose) e de exclusão molecular (Sephadex G-75), sendo o produto desses processos uma banda proteica com elevado grau de pureza, visualizada em SDS-PAGE a 14%, denominada BthMP. Esta, por sua vez, quando analisada em MALDI-TOF revelou a massa molecular nativa de 23.050 Da e 23.872 Da na forma reduzida, e a partir dos fragmentos peptídicos obtidos por Peptide Mass Fingerprinting (PMF) em MS (MALDI-TOF/TOF) indicou alta similaridade com a metaloprotease BmooMPα-I. Em termos enzimáticos, BthMP mostrou atividade proteolítica sobre azocaseína e frente ao PMSF e benzamidina, enquanto que esta atividade foi inibida na presença de EDTA, 1,10-fenantrolina e β-mercaptoetanol, sendo portanto uma metaloprotease zinco dependente da classe P-I. Ainda com este propósito, verificou-se sua especificidade enzimática sobre as cadeias Aα e Bβ do fibrinogênio, e também o consumo de fibrinogênio in vivo. Foi constatado ainda sua ação em componentes da cascata de coagulação, devido ao prolongamento do Tempo de Protrombina (TP) e do Tempo de Tromboplastina Parcial ativada (TTPa). Desta forma, a acentuada atividade fibrinogenolítica e o alto consumo de fibrinogênio in vivo são resultados que indicam a ação anticoagulante da BthMP; além do mais, sua capacidade de interferir na cascata de coagulação sugere que esta protease é promissora para futuros estudos que possam indicar um novo modelo de fármaco antitrombótico.
Seminoma em um cão com testículo ectópico – Relato de caso
Authors: Bomfim Elyse Medeiros Olimpio , Barbosa Yago Gabriel Silva , Baeta Silvia de Araújo Franca , Santos Paulo Victor Garrêto Rodrigues , Viana Felipe José Costa , Correio Francisco Lima Silva
Published: October 2017

As neoplasias testiculares em cães são predominantes em animais com criptorquidismo. Sendo as mais comuns sertolioma e o seminoma. Este último se desenvolve nos túbulos seminíferos, sendo a segunda neoplasia testicular mais encontrada em cães. O diagnóstico é confirmado apenas com a histopatologia dos testículos coletado durante a cirurgia, a qual é a terapia de eleição para retirada do tumor. A remoção dos testículos é a terapia preferencial, pois estudos sobre quimioterápicos para esta neoplasia ainda são escassos. Revisa-se neste estudo o seminoma, através de um relato de caso como também os protocolos terapêuticos escolhidos.
Os fatores genéticos da insônia - Uma revisão de literatura
Authors: Ferreira Guilherme Sousa , Barros Indira Maria de Almeida , Xavier Lyvia Ester Ferreira
Published: October 2017

A insônia é um dos distúrbios do sono caracterizado pela dificuldade em iniciar ou manter o sono. Isso se reflete em um sono de baixa qualidade que pode causar problemas ao longo do dia, como cansaço e falta de energia. Sob essa condição, estão associados alguns fatores genéticos, entre eles os genes ABCC9, 5-HTT e Clock. A presente revisão de literatura tem por objetivo a explanação acerca dos fatores genéticos relacionados com o distúrbio da insônia. Trata-se de uma revisão de literatura de forma sistemática, realizada por meio de um levantamento bibliográfico que procura reunir os conhecimentos sobre as características da insônia e sua herança gênica. Encontrou-se uma relevância de achados autorais valiosos para a síntese dessa pesquisa. Portanto, a partir do estudo abordado nessa revisão, torna-se evidente a importância do conhecimento das causas genéticas do distúrbio da insônia para a melhor abordagem clínica, haja vista a relevância desse mecanismo biológico.
A CAPTURA COMO EXPERIÊNCIA: Investigações pragmáticas e teorias do poder
Authors: CHATEAURAYNAUD Francis
Published: October 2017

Por muito tempo, a sociologia pragmática considerou que não tinha nada a dizer sobre os jogos de poder. Uma de suas regras de método exigia aproximar-se dos próprios atores quando eles se punham a desvelar, ao longo de polêmicas e processos, formas de poder ou de dominação. Apoiando-se em muitos dossiês, o artigo mostra como redirecionar a pesquisa pragmática para apreender de outro modo a crítica das relações de poder, cujo grau de visibilidade pública depende da trajetória seguida pelos atores tomados pela lógica da crise ou do conflito. Os elementos de uma captura bem-sucedida e aqueles em que se apoia todo o trabalho de escape da captura são, pouco a pouco, expostos: imperativo de justificação, conservação de uma necessidade de reconhecimento do caso, controle dos instrumentos de avaliação, produção de uma troca desigual invisível.
Five Efficacious Treatments for Posttraumatic Stress Disorder: An Empirical Review
Authors: Blankenship David M.
Published: October 2017

Posttraumatic stress disorder (PTSD) is known to be a debilitating disorder and for some a lifelong complaint. Although there are many treatment options, determining which treatments are not only recommended but show high efficacy rates is vital for clinicians. This review examines current treatment endorsements for PTSD and identifies five treatment modalities consistently recommended in the literature as most efficacious for PTSD: prolonged exposure therapy, cognitive processing therapy, trauma-focused cognitive behavioral therapy, stress inoculation training, and eye movement desensitization and reprocessing therapy. Comparison studies of each modality, limitations, and training requirements are reviewed. Research overall shows no significant differences in rates of efficacy between treatments, with mental health counselors recommended to select the approach that best fits the client population and professional goals based on identified strengths and limitations of each therapy. Additional recommendations for future directions are discussed.
Nonsuicidal Self-Injury, Interpersonal Neurobiology, and Attachment: Implications for Counselors and Therapists
Authors: De Stefano Jack , Atkins Shawna
Published: October 2017

Given its prevalence, nonsuicidal self-injury (NSSI) is increasingly becoming the focus of mental health counselors and other clinicians. Even when these individuals are knowledgeable about NSSI, the best treatment approaches remain uncertain. While several therapeutic treatments have been evaluated for their effectiveness, results are inconclusive. As the search for best practice continues, the authors propose attachment theory and interpersonal neurobiology as relevant to NSSI, especially considering their potential to facilitate emotion regulation. A case example is provided.
Injury rate in the pediatric population of Saint Petersburg
Authors: Solovyova Karina S. , Zaletina Anna V.
Published: October 2017

Introduction. The injury rate in children remains an urgent and unresolved problem in the field of medicine. In recent years, the average injury rate in 0- to 17-year-old children is 116–118 injuries per 1000 children of the corresponding population in Russia.

Aim. The study aimed to analyze the injury rates in the children in St. Petersburg in 2016 and to compare them with the injury rates of previous years and those of children in Russia.

Material and methods. Based on state statistics, the indices of injury rate in the children in St. Petersburg in 2016 were evaluated. The disability in children as a result of external actions and the activities of medical and preventive institutions for providing specialized care to children with injuries were also investigated.

Results and discussion. A new form of reporting by Rosstat enabled the clarification of the data with respect to the nature of the injuries and the number of bone fractures in various locations of the body in accordance with the XIX class of the ICD-10, as well as with respect to the morbidity and mortality in children owing to external causes (class XX of the ICD) that include road-traffic accidents and physical abuse.

Conclusions. The rate of injury in children aged 0–14 years decreased when compared with that suggested by the previous year’s data. Further, in adolescents aged 15–17 years, the rate of injury was significantly higher than the average rate in Russia. The disability in children as a result of external actions has decreased. However, the prevention of child injuries in St. Petersburg, especially in adolescents, remains an urgent medical and social problem.
Psyсhologiсal defense mechanisms of children with cerebral palsy in the context of mothers attitude to a child’s disease
Authors: Pyatakova Galina V. , Mamajchuk Irina I. , Umnov Valery V.
Published: October 2017

Introduction. Cerebral palsy (CP) has a significant prevalence in industrialized countries and is characterized by a high level of disability. Children and adolescents with CP face challenging situations. Physical defects repeatedly manifest in the daily life of a child in the form of other extraordinary events such as hospitalization, surgery, prolonged immobilization, and separation from family. Such events can be accompanied by feelings of fear, anxiety, helplessness, and pronounced symptoms of maladjustment. Under these conditions, certain biological and social compensatory manifestations help to identify such complicated conditions and restore the normal functioning of the body. Compensatory manifestations are a type of compensatory defense mechanisms. The major role in establishing a system of protection is played by the relationship between a child and his or her parents. Defense mechanisms can be formed during the process of assimilation of protective parental behavior, or because of the inappropriate attitude of mothers to the child’s illness. The purpose of the research was to study the defense mechanisms in children with CP in the context of mothers’ relationship to her child’s illness.

Materials and methods. The research involved 120 people. The experimental group included 30 children with CP aged 5 to 11 years and their mothers. The control group consisted of relatively healthy children (30) and their mothers. The following was used to achieve the goal: the elements of clinical and biographical method, projective technique, the technique of diagnostics of attitude to the child’s illness.

Results and discussion. The repertoire of psychological defense mechanisms in children with CP is limited in comparison with those in healthy children. Children with CP are dominated by the defense mechanism of “denial” that can play a negative role in the process of adaptation of these children in a situation involving complex treatments. The mothers of children with CP share an emotionally intense relationship with their child’s disease. Excessively disturbing attitudes of the mother to the disease of her child can contribute to the formation of negative psychological defense mechanisms of negation type in the child, which reduces the child’s psychological compensatory capacity and capability of adaptation in conditions of complex treatment.
Surgical treatment of gross posttraumatic deformations in thoracic spine
Authors: Shul’ga Aleksei E. , Zaretskov Vladimir V. , Korshunova Galina A. , Smol'kin Aleksei A. , Sumin Dmitrii Yu.
Published: October 2017

Rigid severe post-traumatic thoracic spine deformities result from frequent, recent high-energy trauma in children with an increasing frequency due to a variety of reasons. These types of injuries are commonly followed by spinal cord anatomic injury; therefore, the treatment of these patients warrants special attention from the ethical viewpoint. Generally, the only indication for surgical intervention is spinal dysfunction. Considering this and the patients’ ordinary severe somatic state, surgical trauma should be minimized as much as possible. However, for adequate deformity correction, effective spine stabilization and restoration of liquorodynamics is necessary. Recent studies have reported the successful use of different methods of dorsal interventions (P/VCR) in cases with unstable damages in children. Here, we present the case of a 15-year-old boy who underwent surgical treatment for coarse post-traumatic thoracic spine deformity with chronic fracture-dislocation of Th7 vertebra.
Runoff generation in mountain catchments: long-term hydrological monitoring in the Rio Vauz Catchment, Italy
Authors: Zuecco G. , Penna D. , Borga M.
Published: October 2017

Trying to obtain a more detailed understanding of the hydrological functioning of mountain catchments represents an important challenge in the effort of counteracting possible consequences of climate and land use change on water resources availability. Long-term (> 10 years) hydro-meteorological monitoring in small (typically < 10 km2) experimental catchments constitutes a valuable tool to achieve these goal. One of these sites is the Rio Vauz Catchment (1.9 km2), in the Italian Dolomites, that represents an excellent example of long-term snowmelt-dominated catchment in Dolomitic regions. The strong elevation gradient of the Rio Vauz Catchment and the different physiographic properties of its nested subcatchments make this a unique site for investigating fundamental runoff generation mechanisms in mountain headwaters. In this work, we provide a review of physical processes that have been inferred from 12 years of hydrological monitoring in this catchment. We present the available dataset and summarize the main hydrological mechanisms that explain the internal functioning of the Rio Vauz Catchment, primarily focusing on three characterizing hydrological behaviours, namely thresholds, hysteresis and connectivity. The main control on surface and subsurface runoff threshold response is constituted by a combination of soil moisture antecedent conditions, rainfall amount and topography. Changes in hysteresis patterns (clockwise and anti-clockwise loops) between streamflow and soil moisture, water table depth and electrical conductivity were governed by distinct runoff generation processes and rainfall event characteristics. Hillslope-riparian-stream subsurface connectivity was controlled by antecedent wetness conditions and rainfall amount. The composition in environmental tracers (stable isotopes of water and electrical conductivity) in different water sources and the application of tracer-based mixing models helped to distinguish the geographical sources to runoff and to quantify the role of rainfall and snowmelt in streamflow. Finally, we define a perceptual model of runoff generation processes for dry and wet conditions that can be considered representative for many mountain headwater catchments in the world.
Measurement of peak expiratory flow rate values in healthy school going children between 6 and 12 years attending urban schools in Chennai
Authors: Durairaj Prabakar , Raju Sasivarathan , Thirumalaikumarasamy Sivaraman
Published: October 2017

Background: Peak expiratory flow rate (PEFR) is an important tool in monitoring patient’s changes in asthma. Bronchial asthma is among the commonest disorders in childhood. Here authors planned to measure PEFR in healthy school going children between 6 and 12 years and correlate PEFR against various parameters such as height, weight, sex, age and chest circumference.Methods: The study was conducted from February 2005-August 2006 among healthy children between 6 and 12 years of age attending urban schools of both sexes in Chennai. The schools were selected randomly. Results: PEFR values were measured in a large number of children between 6 and 12 years and varied significantly between boys and girls in the age group 6 to 12 years. Among all the study variables, height had shown the maximum positive correlation to PEFR in both boys and girls. The co-efficient of regression derived for height was found to be highly statistically significant both in boys and girls.Conclusions: Significant correlations were found between PEFR and biological variables like age, weight, height and chest circumference. The correlation is more robust with regard to height. Boys have more PEFR values than girls across all age groups.
Entre realidade e ficção: o desafio de Dom Quixote a Erich Auerbach e Jacques Rancière * Between reality and fiction: the challenge of Don Quixote to Erich Auerbach and Jacques Rancière
Authors: Mendonça Martins Miriam
Published: October 2017

O presente artigo objetiva apresentar as considerações que Erich Auerbach e Jacques Rancière produziram a respeito do livro Dom Quixote de Miguel de Cervantes. Enquanto o primeiro defende que esta obra destoa da literatura moderna devido ao fato de não representar a realidade de maneira trágica, o segundo, por sua vez, argumenta que o referido romance sinaliza o advento de uma literatura que rompe com a mímesis clássica ao demonstrar a impossibilidade de correspondência entre as palavras e as coisas.
Prometeus desacorrentados: a influência do discurso nacionalista dos anos 60 no processo de (des)construção do jazz brasileiro * Prometheus unleashed: the nationalism speech influence in the process of (de)construction of brazilian jazz
Authors: Ferreira Junior José , Ribeiro Junior Antonio Carlos Araújo
Published: October 2017

O artigo faz uma análise inicial do discurso nacionalista dos anos 1960, tentando investigar até que ponto os embates políticos e ideológicos desse período influíram nos rumos do jazz brasileiro. Uma vez que não se tem um consenso até hoje no âmbito da crítica musical para denominar e caracterizar o jazz tocado no Brasil, divergindo-se entre os rótulos “jazz brasileiro” e “música instrumental brasileira”. Acredita-se que alguns fatores contribuíram para esse cenário, por exemplo: o conservadorismo da crítica contra o teor antropofágico dos músicos brasileiros; o estabelecimento da canção popular, e de seu aspecto político, como fator fundamental de autenticidade musical; e, por fim, a ideia de um jazz como música alienante, corroborada pela intelligentsia da Música Popular Brasileira – conhecida como MPB, conceito hegemônico a partir da segunda metade do século XX.
Apresentação do Dossiê Assistência e Pobreza: sentidos e lugares dos pobres no Brasil
Authors: Rossi Daiane Silveira , Ribeiro Lidiane Monteiro
Published: October 2017

Texto de Apresentação do Dossiê Assistência e Pobreza: sentidos e lugares dos pobres no Brasil. V.6, N.2, 2017.
Cortejo de miséria: seca, assistência e mortalidade infantil na segunda metade do século XIX no Ceará * Courtege of misery: drought, assistance and infant mortality in the second half of the 19th century in Ceará
Authors: Gadelha Georgina Da Silva , Lima Zilda Maria Menezes
Published: October 2017

A História do Ceará é marcada pelas secas e suas consequências que geraram (e geram) migrações e mortalidade humana expressiva. Nosso objetivo é analisar a segunda metade do século XIX, sobretudo a seca de 1877-1879, conhecida como a “grande seca”, buscando compreender como o governo, a partir desse período, passou a entender a pobreza, a assistência e a caridade destinadas aos socorros dos migrantes que saíram do campo para os centros urbanos à procura de auxílio do poder público, o qual atuou dentro da perspectiva do controle e da disciplina. Procuramos demonstrar também a elevação significativa nas taxas de mortalidade infantil por terem sido as crianças, a parte da população que mais sofreu com a fome e as altas temperaturas e pelo tema ser uma questão pouco explorada na literatura cearense.
Sobre las posiciones etnográficas en la antropología de la salud en el sur de las Américas
Authors: Epele María
Published: October 2017

Las etnografías sobre problemas de salud en poblaciones que viven bajo condiciones de desigualdad, pobreza y segregación han proliferado en Sudamérica durante las últimas décadas. El objetivo de este trabajo consiste en problematizar, de forma preliminar, ciertos patrones en las relaciones y posiciones de los etnógrafos respecto de los sujetos y de las poblaciones, tanto en el trabajo de campo como en la escritura de los resultados. Analizando dichas posiciones, este artículo examina su relación con las orientaciones teóricas dominantes en la región. Además, explora, brevemente, los poderes de resolución, las sensibilidades, los mapas teóricos y de los sentidos de algunas posiciones a la luz de los desafíos de las lógicas de poder, las economías simbólicas y las diversas variantes de acumulación por desposesión dominantes en esta geográfica.