bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association Between Decompensated Liver Cirrhosis and Deep Neck Infection: Real-World Evidence
Authors: Tsai Ming-Shao , Chang Geng-He , Chen Wei-Ming , Liu Chia-Yen , Lin Meng-Hung , Chang Pey-Jium , Huang Tsung-Yu , Tsai Yao-Te , Wu Ching-Yuan , Hsu Cheng-Ming , Yang Yao-Hsu
Published: October 2019

Background: Deep neck infection (DNI) can progress to become a life-threatening complication. Liver cirrhosis, which is related to poor immune conditions, is a likely risk factor for DNI. This study investigated the risk and mortality of DNI in patients with decompensated liver cirrhosis (DLC). Methods: We performed a nationwide cohort study using the National Health Insurance Research Database (NHIRD) in Taiwan. We included a total of 33,175 patients with DLC between 2000 and 2013, from the Catastrophic Illness Patient Database, a subsection of the NHIRD, along with 33,175 patients without cirrhosis who were matched in a 1:1 proportion for age, sex, and socioeconomic status. The occurrence of DNI was the primary study outcome. The risk, treatment, and mortalities of DNI were evaluated in the study and comparison cohorts. Results: DLC Patients had a significantly higher incidence of DNI than noncirrhotic patients (p < 0.001). The adjusted Cox proportional hazard regression showed that DLC was associated with a significantly higher risk of DNI (adjusted hazard ratio, 4.11; 95% confidence interval, 3.16–5.35, p < 0.001). The mortality rate in cirrhotic patients with DNI was not significantly higher than that in noncirrhotic patients with DNI (11.6% vs. 9.8%; p = 0.651). Conclusions: This study is the first to investigate the correlation between DLC and DNI. The study findings strongly indicate that DLC is an independent risk factor for DNI. Cirrhotic patients with DNI do not have a significantly poorer survival rate than noncirrhotic patients with DNI. Therefore, physicians should be alert to potential DNI occurrence in DLC patients. Besides this, intensive care and appropriate surgical drainage can yield similar survival outcomes in DLC-DNI and noncirrhosis-DNI patients.
Household Smoking Status and Heavy Metal Concentrations in Toenails of Children
Authors: Karatela Shamshad , Coomarasamy Christin , Paterson Janis , Ward Neil I.
Published: October 2019

There is limited evidence on the distribution of heavy metals and its association with secondhand smoking (SHS) within Pacific Island children living in New Zealand. Certain heavy metals such as cadmium (Cd), lead (Pb), and aluminum (Al) bioaccumulates in the body and can deteriorate health in both children and adults. Others, such as chromium (Cr) and nickel (Ni) in trace amounts are necessary but become toxic at high levels. Exposure routes of these elements include food, water, and air. The purpose of this study was to identify the distribution of toxic metal concentrations and its possible correlation with SHS within the Pacific Island children. A sub-sample of children within Pacific Island families longitudinal study, at the nine-year phase, who were living in the New Zealand city of Auckland were invited to participate, (n = 278). Toenails were used as a biomarker to determine Cr, Pb, Cd, Cu, Ni, and Al concentration using inductively coupled plasma mass spectrometry. Reliable and validated questionnaires were used for demographics, lifestyle, and health outcome variables. Significant differences between household smoking status and ethnicity, as well as parents’ marital status, were observed (p < 0.05). There was no statistical difference in heavy metal concentrations in smoking versus non-smoking households. However, Cr, Pb, Cd, Cu, and Ni concentrations were all higher than the required optimal health value in both groups. A high concentration of heavy metals was observed in these children that exceeded the value required for optimal health, although no significant difference in heavy metals with regards to secondhand smoking was observed. SHS was associated with children’s ethnicity and parental marital status, but not with household income levels or maternal education.
Topographic and Biomechanical Changes after Application of Corneal Cross-Linking in Recurrent Keratoconus
Authors: Pedrotti Emilio , Caldarella Grazia , Fasolo Adriano , Bonacci Erika , Gennaro Nicola , De Gregorio Alessandra , Marchini Giorgio
Published: October 2019

Background: Recurrent keratoconus (RKC) develops as a progressive thinning of the peripheral and the inferior cornea after keratoplasty, in both graft and host, causing secondary astigmatism, refractive instability, and reduced visual acuity. We evaluated the effectiveness of corneal cross-linking (CXL) in patients diagnosed with RKC. Methods: Accelerated-CXL via the epi-off technique was performed in15 patients (18 eyes) diagnosed with RKC. Topographic and biomechanical changes were assessed at 12 months. Results: Differences in maximum keratometry, thinnest corneal thickness, and biomechanical parameters (deformation amplituderatio, inverse concave radius, applanation 1 velocity, and applanation 2 velocity, stiffness A1) versus baseline were statistically significant (p < 0.05).Best corrected visual acuity was improved in 13 eyes and unchanged in 4;manifest refractive spherical equivalent was reduced in 13 eyes, increased in 3,and unchanged in 1 eye; topographic astigmatism was reduced in 9 eyes, remained stable in 1 eye, and increased in 7 eyes. Conclusions: Improved topographic and biomechanic indexes at 1 year after CXL suggest it‘s potential as first-line therapy for RKC, as it is for KC.
Quality of Life and Work Ability among Healthcare Personnel with Chronic Viral Hepatitis. Evaluation of the Inpatient Rehabilitation Program of the Wartenberg Clinic
Authors: Westermann Claudia , Nienhaus Albert , Treszl András
Published: October 2019

The aim of this study was to research the impact of inpatient rehabilitation on work ability and health-related quality of life factors for healthcare personnel (HP) with chronic hepatitis B and C virus (HBV and HCV) infection. A prospective evaluation study with three data collection times without an external control group was conducted. HP (n = 163) with an occupational acquired chronic hepatitis B/C infection who participated in an inpatient rehabilitation program were surveyed. Information was collected on work ability (WAI—Work Ability Index), quality of life (SF-36—Short Form-36 Health Survey), and anxiety and depression-related symptoms (HADS-D—Hospital Anxiety and Depression Scale). The majority of participants had HCV infection. Work ability was poor, improved significantly until the end of treatment, and remained at a moderate level six months later. The SF-36 showed no change in physical health over the study period, the results regarding mental health were in the average range with a significant improvement directly after intervention. The HADS-D results indicate noteworthy anxiety and depression symptoms during the study period. The inpatient rehabilitation program proved to be effective in the short term regarding mental health (SF-36) and WAI. To ensure long lasting positive results, services aimed at enhancing physical and mental health should be provided as early as possible and on a recurring basis.
Effects of Three Months of Detraining on the Health Profile of Older Women after a Multicomponent Exercise Program
Authors: Leitão Luis , Pereira Ana , Mazini Mauro , Venturini Gabriela , Campos Yuri , Vieira João , Novaes Jefferson , Vianna Jeferson , da Silva Sandro , Louro Hugo
Published: October 2019

Physical exercise results in very important benefits including preventing disease and promoting the quality of life of older individuals. Common interruptions and training cessation are associated with the loss of total health profile, and specifically cardiorespiratory fitness. Would detraining (DT) promote different effects in the cardiorespiratory and health profiles of trained and sedentary older women? Forty-seven older women were divided into an experimental group (EG) and a control group (CG) (EG: n = 28, 70.3 ± 2.3 years; CG: n = 19, 70.1 ± 5.6 years). Oxygen uptake (VO2) and health profile assessments were conducted after the exercise program and after three months of detraining. The EG followed a nine-month multicomponent exercise program before a three-month detraining period. The CG maintained their normal activities. Repeated measures ANOVA showed significant increases in total heath and VO2 (p < 0.01) profile over a nine-month exercise period in the EG and no significant increases in the CG. DT led to greater negative effects on total cholesterol (4.35%, p < 0.01), triglycerides (3.89%, p < 0.01), glucose (4.96%, p < 0.01), resting heart rate (5.15%, p < 0.01), systolic blood pressure (4.13%, p < 0.01), diastolic blood pressure (3.38%, p < 0.01), the six-minute walk test (7.57%, p < 0.01), Pulmonary Ventilation (VE) (10.16%, p < 0.01), the Respiratory Exchange Ratio (RER) (9.78, p < 0.05), and VO2/heart rate (HR) (16.08%, p < 0.01) in the EG. DT may induce greater declines in total health profile and in VO2, mediated, in part, by the effectiveness of multicomponent training particularly developed for older women.
The Perception and Intervention of Internship Nursing Students Helping Smokers to Quit: A Cross-Sectional Study in Chongqing, China
Authors: Zhang Li , Chen Yanhan , Lv Yalan , Yang Xia , Yin Qianyu , Bai Li , Luo Yaling , Sharma Manoj , Zhao Yong
Published: October 2019

Background: Smoking is among the most preventable causes of death globally. Tobacco cessation can lessen the number of potential deaths. The China Tobacco Cessation Guidelines encourage medical staff to perform the 5As (Ask, Advise, Assess, Assist, Arrange) when delivering tobacco dependence treatments to patients. Nursing students will develop to be nurses in the future and they have to finish 9 months of clinical practicum study in the last year at hospitals or care centers. However, the frequency of behaviors used to help smokers quit among Chinese nursing internship students is unclear. This study analyzed the rate of nurse interns’ performance of the 5As and which demographic characteristics, perceptions of smoking and knowledge predicted higher performance of the 5As. Methods: The cluster sampling method was used to select 13 teaching hospitals among 29. All nursing intern students were expected to finish the questionnaire about their 5As behaviors to help patients quit smoking. Their 5As performances were scored from one to five with 5 being the best and scores were summed. A multivariate linear mixed-effect model was employed to test the differences between their 5As. Results: Participating in the survey were 1358 interns (62.4% response rate). The average scores were as follows—Ask—3.15, Advise—2.75, Assess—2.67, Assist—2.58 and Arrange—2.42. A total of 56.3% students perceived that medical staff should perform the 5As routinely to help patients quit smoking. On the other hand, 52.1% viewed clinical preceptors as role models of the 5As. School education regarding tobacco control, smoking dependence treatment, self-efficacy and positive intentions were predictors of higher performance of the 5As (p < 0.001). Conclusions: Nursing internship students seldom administered tobacco dependence treatments to patients. It is essential to improve the corresponding education, skills and self-efficacy of the 5As. Meanwhile, clinical preceptors should procure more training in the responsibilities and skills related to tobacco cessation. In this way, clinical preceptors can be role models of the 5As and impart positive influences on interns.
Isotope Composition and Chemical Species of Monthly Precipitation Collected at the Site of a Fusion Test Facility in Japan
Authors: Akata Naofumi , Tanaka Masahiro , Iwata Chie , Kato Akemi , Nakada Miki , Kovács Tibor , Kakiuchi Hideki
Published: October 2019

The deuterium plasma experiment was started using the Large Helical Device (LHD) at the National Institute for Fusion Science (NIFS) in March 2017 to investigate high-temperature plasma physics and the hydrogen isotope effects towards the realization of fusion energy. In order to clarify any experimental impacts on precipitation, precipitation has been collected at the NIFS site since November 2013 as a means to assess the relationship between isotope composition and chemical species in precipitation containing tritium. The tritium concentration ranged from 0.10 to 0.61 Bq L−1 and was high in spring and low in summer. The stable isotope composition and the chemical species were unchanged before and after the deuterium plasma experiment. Additionally, the tritium concentration after starting the deuterium plasma experiment was within three sigma of the average tritium concentration before the deuterium plasma experiment. These results suggested that there was no impact by tritium on the environment surrounding the fusion test facility.
Multidimensional Health Groups and Healthcare Utilization Among Elderly Chinese: Based on the 2014 CLHLS Dataset
Authors: Ye Linglong , Luo Jiecheng , Shia Ben-Chang , Fang Ya
Published: October 2019

Based on multidimensional health, we aimed to identify health groups among the elderly Chinese population, and examine its relationship with socio-demographic factors on healthcare utilization. Chinese Longitudinal Healthy Longevity Survey in 2014 was adopted. For 2981 participants aged ≥65 years, without missing any health indicators, latent class analysis was adopted to identify health groups. For 1974 participants with complete information, the two-part model was used to assess how health groups and socio-demographic characteristics influence the outpatient and inpatient expenditure. Four health groups were identified and labeled as “Lacking Socialization” (10.4%), “High Comorbidity” (16.7%), “Severe Disability” (7.8%), and “Relative Health” (65.1%). Compared with the relative health group, the lacking socialization group cost higher inpatient expenditure (p = 0.02). Those in the high comorbidity and severe disability groups were more likely to use healthcare services and cost higher outpatient expenditure (p < 0.01 for all). The effects of socio-demographic factors were also discussed. The findings enhanced our understanding of the heterogeneity of multidimensional health status and complex healthcare demands in the elderly Chinese population. Moreover, it is valuable for improving the allocation of healthcare resource targeted for different groups of the ageing population.
Age-Period-Cohort Analysis on the Time Trend of Hepatitis B Incidence in Four Prefectures of Southern Xinjiang, China from 2005 to 2017
Authors: Ji Weidong , Xie Na , He Daihai , Wang Weiming , Li Hui , Wang Kai
Published: October 2019

Objective: The influence of age, period, and cohort on Hepatitis B (HB) incidence in four prefectures of southern Xinjiang, China is still not clear. This paper aims to analyze the long-term trend of the HB incidence in four prefectures of southern Xinjiang, China and to estimate the independent impact of age, period and cohort, as well as to predict the development trend of HB incidence in male and female groups, then to identify the targeted population for HB screening by the model fitting and prediction. Method: The data were from the Case List of HB Cases Reported in the Infectious Disease Reporting Information Management System and the Xinjiang Statistical Yearbook of China. The age-period-cohort (APC) model was used to estimate the impacts of age, period and cohort on HB incidence, which could be used to predict the HB incidence in specific age groups of men and women. Results: Under the influence of age effect, the incidence of HB in males had two peaks (20–35 years old and 60–80 years old), the influence of age effect on the incidence of HB in females was lower than that of males and the obvious peak was between 20–30 years old; the period effect on the HB incidence in males and females fluctuated greatly and the fluctuation degree of influence on males was bigger than that of women. The HB incidence among males and females in the four regions tended to be affected by cohort effect, which reached a peak after 1990 and then declined sharply and gradually became stabilized. By predicting the HB incidence from 2018 to 2022, we found that there were significant differences in HB incidence among people over 35 years old, under 35 years old and the whole population in four prefectures of southern Xinjiang, China. Conclusions: Although the incidence of HB in some regions shows a downward trend, there is still an obvious upward trend of incidences in other places. In our paper, results indicate that the burden of HB incidence may be extended in the future, so we hope this can draw the attention of relative departments. These results reveal the differences of incidence between males and females as well, so respective measures of the two groups’ functions are essential.
Influencing Factors of PM2.5 Pollution: Disaster Points of Meteorological Factors
Authors: Sun Ruiling , Zhou Yi , Wu Jie , Gong Zaiwu
Published: October 2019

A chance constrained stochastic Data Envelopment Analysis (DEA) was developed for investigating the relations between PM2.5 pollution days and meteorological factors and human activities, incorporating with an empirical study for 13 cities in Jiangsu Province (China) to illustrate the model. This approach not only admits random input and output environment, but also allows the evaluation unit to exceed the front edge under the given probability constraint. Moreover, observing the change in outcome variables when a group of explanatory variables are deleted provides an additional strategic technique to measure the effect of the remaining explanatory variables. It is found that: (1) For 2013–2016, the influencing factors of PM2.5 pollution days included wind speed, no precipitation day, relative humidity, population density, construction area, transportation, coal consumption and green coverage rate. In 2016, the number of cities whose PM2.5 pollution days was affected by construction was decreased by three from 2015 but increased according to transportation and energy utilization. (2) The PM2.5 pollution days in southern and central Jiangsu Province were primarily affected by the combined effect of the meteorological factors and social progress, while the northern Jiangsu Province was largely impacted by the social progress. In 2013–2016, at different risk levels, 60% inland cities were of valid stochastic efficiency, while 33% coastal cities were of valid stochastic efficiency. (3) The chance constrained stochastic DEA, which incorporates the data distribution characteristics of meteorological factors and human activities, is valuable for exploring the essential features of data in investigating the influencing factors of PM2.5.
Sustainable Agriculture by Increasing Nitrogen Fertilizer Efficiency Using Low-Resolution Camera Mounted on Unmanned Aerial Vehicles
Authors: Kim Dong-Wook , Min Tae-Sun , Kim Yoonha , Silva Renato Rodrigues , Hyun Hae-Nam , Kim Ju-Sung , Kim Kyung-Hwan , Kim Hak-Jin , Chung Yong Suk
Published: October 2019

Nitrogen use efficiency in modern agriculture is very low. It means that a lot of synthetic chemicals are wasted rather than utilized by crops. This can cause more problems where the soil surface is thin and rocky like Jeju Island in the Republic of Korea. This is because overly used nitrogen fertilizer can be washed into the underground water and pollute it. Thus, it would be important to monitor the nitrogen deficiency of crops in the field to provide the right amount of nitrogen in a timely manner so that nitrogen waste can be limited. To achieve this, the normalized difference vegetation index (NDVI) was used to monitor chlorophyll content, which is tightly associated with nitrogen content in the buckwheat field. The NDVI was calculated with the data obtained by a low-resolution camera mounted on an unmanned aerial vehicle. The results showed that the NDVI can estimate the chlorophyll content of buckwheat. These simple but clear results imply that precision agriculture could be achieved even with a low-resolution camera in a cost-effective manner to reduce the pollution of underground water.
Pain Intensity among Community-Dwelling African American Older Adults in an Economically Disadvantaged Area of Los Angeles: Social, Behavioral, and Health Determinants
Authors: C. Evans Meghan , Bazargan Mohsen , Cobb Sharon , Assari Shervin
Published: October 2019

Background. Although social, behavioral, and health factors influence prevalence and intensity of pain, very few studies have investigated correlates of pain among economically disadvantaged older African American (AA) adults. Objective. This study explored social, behavioral, and health correlates of pain intensity among community-dwelling AA older adults in an economically disadvantaged area of Los Angeles. Methods. A cross-sectional study on 740 AA older adults (age ≥ 55 years) was conducted in South Los Angeles between 2015 and 2018. Exploratory variables were age, gender, educational attainment, financial difficulties, living alone, marital status, smoking, drinking, pain-related chronic medical conditions (CMCs), and depressive symptoms. Dependent variable was pain intensity. Linear regression was used for data analysis. Results. Age, financial difficulties, living alone, smoking, pain-related chronic medical conditions, and depressive symptoms were associated with pain intensity. Individuals with lower age, higher financial difficulties, those who lived alone, those with a higher number of pain-related chronic medical conditions, more depressive symptoms, and nonsmokers reported more pain intensity. Gender, educational attainment, marital status, and drinking were not associated with pain intensity. Conclusion. The results may help with the health promotion of economically disadvantaged AA older adults in urban areas.
PPARγ and Cognitive Performance
Authors: d’Angelo Michele , Castelli Vanessa , Catanesi Mariano , Antonosante Andrea , Dominguez-Benot Reyes , Ippoliti Rodolfo , Benedetti Elisabetta , Cimini Annamaria
Published: October 2019

Recent findings have led to the discovery of many signaling pathways that link nuclear receptors with human conditions, including mental decline and neurodegenerative diseases. PPARγ agonists have been indicated as neuroprotective agents, supporting synaptic plasticity and neurite outgrowth. For these reasons, many PPARγ ligands have been proposed for the improvement of cognitive performance in different pathological conditions. In this review, the research on this issue is extensively discussed.
Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells
Authors: Aghajani Nargesi Arash , Zhu Xiang-Yang , Liu Yuanhang , Tang Hui , Jordan Kyra L. , Lerman Lilach O. , Eirin Alfonso
Published: October 2019

Background: Scattered tubular-like cells (STCs) proliferate and differentiate to support neighboring injured renal tubular cells during recovery from insults. Renal artery stenosis (RAS) induces renal ischemia and hypertension and leads to loss of kidney function, but whether RAS alters renal endogenous repair mechanisms, such as STCs, remains unknown. We hypothesize that RAS in swine modifies the messenger RNA (mRNA) profile of STCs, blunting their in vitro reparative capacity. Methods: CD24+/CD133+ STCs were isolated from pig kidneys after 10-weeks of RAS or sham (n = 3 each) and their gene cargo analyzed using high-throughput mRNAseq. Expression profiles for upregulated and downregulated mRNAs in RAS-STCs were functionally interpreted by gene ontology analysis. STC activation was assessed by counting the total number of STCs in pig kidney sections using flow cytometry, whereas cell proliferation was assessed in vitro. Results: Of all expressed genes, 1430 genes were upregulated and 315 downregulated in RAS- versus Normal-STCs. Expression of selected candidate genes followed the same fold change directions as the mRNAseq findings. Genes upregulated in RAS-STCs were involved in cell adhesion, extracellular matrix remodeling, and kidney development, whereas those downregulated in RAS-STCs are related to cell cycle and cytoskeleton. The percentage of STCs from dissociated kidney cells was higher in RAS versus Normal pigs, but their proliferation rate was blunted. Conclusions: Renal ischemia and hypertension in swine induce changes in the mRNA profile of STCs, associated with increased STC activation and impaired proliferation. These observations suggest that RAS may alter the reparative capacity of STCs.
Generation of a Nebulizable CDR-Modified MERS-CoV Neutralizing Human Antibody
Authors: Kim Sang Il , Kim Sujeong , Kim Jinhee , Chang So Young , Shim Jung Min , Jin Jongwha , Lim Chungsu , Baek Songyi , Min Ji-Young , Park Wan Beom , Oh Myoung-don , Kim Seungtaek , Chung Junho
Published: October 2019

Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe aggravating respiratory failure in infected patients, frequently resulting in mechanical ventilation. As limited therapeutic antibody is accumulated in lung tissue following systemic administration, inhalation is newly recognized as an alternative, possibly better, route of therapeutic antibody for pulmonary diseases. The nebulization process, however, generates diverse physiological stresses, and thus, the therapeutic antibody must be resistant to these stresses, remain stable, and form minimal aggregates. We first isolated a MERS-CoV neutralizing antibody that is reactive to the receptor-binding domain (RBD) of spike (S) glycoprotein. To increase stability, we introduced mutations into the complementarity-determining regions (CDRs) of the antibody. In the HCDRs (excluding HCDR3) in this clone, two hydrophobic residues were replaced with Glu, two residues were replaced with Asp, and four residues were replaced with positively charged amino acids. In LCDRs, only two Leu residues were replaced with Val. These modifications successfully generated a clone with significantly greater stability and equivalent reactivity and neutralizing activity following nebulization compared to the original clone. In summary, we generated a MERS-CoV neutralizing human antibody that is reactive to recombinant MERS-CoV S RBD protein for delivery via a pulmonary route by introducing stabilizing mutations into five CDRs.
Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma
Authors: Cao Mingzhuo , Gao Yong , Zhan Mengling , Qiu Nasha , Piao Ying , Zhou Zhuxian , Shen Youqing
Published: October 2019

In the last 2–3 decades, gene therapy represented a promising option for hepatocellular carcinoma (HCC) treatment. However, the design of safe and efficient gene delivery systems is still one of the major challenges that require solutions. In this study, we demonstrate a versatile method for covalent conjugation of glycyrrhizin acid (GL) or glycyrrhetinic acid (GA) to increase the transfection efficiency of Polyethyleneimine (PEI, Mw 1.8K) and improve their targeting abilities of hepatoma carcinoma cells. GA and GL targeting ligands were grafted to PEI via N-acylation, and we systematically investigated their biophysical properties, cytotoxicity, liver targeting and transfection efficiency, and endocytosis pathway trafficking. PEI-GA0.75, PEI-GL10.62 and PEI-GL20.65 conjugates caused significant increases in gene transfection efficiency and superior selectivity for HepG2 cells, with all three conjugates showing specific recognition of HepG2 cells by the free GA competition assay. The endocytosis inhibition and intracellular trafficking results indicated that PEI-GA0.75 and GL10.62 conjugates behaved similarly to SV40 virus, by proceeding via the caveolae- and clathrin-independent mediated endocytosis pathway and bypassing entry into lysosomes, with an energy independent manner, achieving their high transfection efficiencies. In the HepG2 intraperitoneal tumor model, PEI-GA0.75 and PEI-GL10.62 carrying the luciferase reporter gene gained high gene expression, suggesting potential use for in vivo application.
Thrombin Upregulates PAI-1 and Mesothelial–Mesenchymal Transition Through PAR-1 and Contributes to Tuberculous Pleural Fibrosis
Authors: Hsieh Cheng-Ying , Sheu Joen-Rong , Yang Chih-Hao , Chen Wei-Lin , Tsai Jie-Heng , Chung Chi-Li
Published: October 2019

Thrombin is an essential procoagulant and profibrotic mediator. However, its implication in tuberculous pleural effusion (TBPE) remains unknown. The effusion thrombin and plasminogen activator inhibitor-1 (PAI-1) levels were measured among transudative pleural effusion (TPE, n = 22) and TBPE (n = 24) patients. Pleural fibrosis, identified as radiological residual pleural thickening (RPT) and shadowing, was measured at 12-month follow-up. Moreover, in vivo and in vitro effects of thrombin on PAI-1 expression and mesothelial–mesenchymal transition (MMT) were assessed. We demonstrated the effusion thrombin levels were significantly higher in TBPE than TPE, especially greater in TBPE patients with RPT > 10mm than those without, and correlated positively with PAI-1 and pleural fibrosis area. In carbon black/bleomycin-treated mice, knockdown of protease-activated receptor-1 (PAR-1) markedly downregulated α-smooth muscle actin (α-SMA) and fibronectin, and attenuated pleural fibrosis. In pleural mesothelial cells (PMCs), thrombin concentration-dependently increased PAI-1, α-SMA, and collagen I expression. Specifically, Mycobacterium tuberculosis H37Ra (MTBRa) induced thrombin production by PMCs via upregulating tissue factor and prothrombin, and PAR-1 silencing considerably abrogated MTBRa−stimulated PAI-1 expression and MMT. Consistently, prothrombin/PAR-1 expression was evident in the pleural mesothelium of TBPE patients. Conclusively, thrombin upregulates PAI-1 and MMT and may contribute to tuberculous pleural fibrosis. Thrombin/PAR-1 inhibition may confer potential therapy for pleural fibrosis.
Green Tea Polyphenol (−)-Epigallocatechin Gallate (EGCG) Attenuates Neuroinflammation in Palmitic Acid-Stimulated BV-2 Microglia and High-Fat Diet-Induced Obese Mice
Authors: Mao Limin , Hochstetter Danielle , Yao Liyun , Zhao Yueling , Zhou Jihong , Wang Yuefei , Xu Ping
Published: October 2019

Obesity is closely associated with neuroinflammation in the hypothalamus, which is characterized by over-activated microglia and excessive production of pro-inflammatory cytokines. The present study was aimed at elucidating the effects of (−)-epigallocatechin gallate (EGCG) on palmitic acid-stimulated BV-2 microglia and high-fat-diet-induced obese mice. The results indicated the suppressive effect of EGCG on lipid accumulation, pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) release, and microglial activation in both cellular and high-fat-diet rodent models. These results were associated with lower phosphorylated levels of the janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) signaling pathway. In conclusion, EGCG can attenuate high-fat-induced hypothalamic inflammation via inhibiting the JAK2/STAT3 signaling pathways in microglia.
Evidences from a Systematic Review and Meta-Analysis Unveil the Role of MiRNA Polymorphisms in the Predisposition to Female Neoplasms
Authors: Bastami Milad , Choupani Jalal , Saadatian Zahra , Zununi Vahed Sepideh , Ouladsahebmadarek Elaheh , Mansoori Yasser , Daraei Abdolreza , Samadi Kafil Hossein , Yousefi Bahman , Mahdipour Mahdi , Masotti Andrea , Nariman-Saleh-Fam Ziba
Published: October 2019

Breast (BCa) and gynecological (GCa) cancers constitute a group of female neoplasms that has a worldwide significant contribution to cancer morbidity and mortality. Evidence suggests that polymorphisms influencing miRNA function can provide useful information towards predicting the risk of female neoplasms. Inconsistent findings in the literature should be detected and resolved to facilitate the genetic screening of miRNA polymorphisms, even during childhood or adolescence, and their use as predictors of future malignancies. This study represents a comprehensive systematic review and meta-analysis of the association between miRNA polymorphisms and the risk of female neoplasms. Meta-analysis was performed by pooling odds-ratios (ORs) and generalized ORs while using a random-effects model for 15 miRNA polymorphisms. The results suggest that miR-146a rs2910164 is implicated in the susceptibility to GCa. Moreover, miR-196a2 rs11614913-T had a moderate protective effect against female neoplasms, especially GCa, in Asians but not in Caucasians. MiR-27a rs895819-G might pose a protective effect against BCa among Caucasians. MiR-499 rs3746444-C may slightly increase the risk of female neoplasms, especially BCa. MiR-124 rs531564-G may be associated with a lower risk of female neoplasms. The current evidences do not support the association of the remaining polymorphisms and the risk of female neoplasms.
Approach of Agile Methodologies in the Development of Web-Based Software
Authors: Molina Ríos Jimmy , Pedreira-Souto Nieves
Published: October 2019

The current inclusion of agile methodologies in web-oriented projects has been considered on a large-scale by software developers. However, the benefits and limitations go beyond the comforts that project managers delimit when choosing them. Selecting a methodology involves more than only the associated processes or some documentation. Based on the above, we could define as the main concerns the approach with which we identify the methodology, the needs of the company, the size, and qualities of the project, and especially the characteristics of agile development that they possess. However, there are several difficulties in selecting the most appropriate methodology due to the features in common; Will it be suitable for my project? What challenges will be presented in the process? Will my team understand each stage? Will I be able to deliver software that satisfies the client? Project managers create these questions, which seem manageable but have huge effects. This paper presents a systematic literature review based on the analysis of the approaches of six web development methodologies. The aim of the study is to analyze the approaches presented by relevant methodologies, identifying their common agile characteristics and managing to contrast both its benefits and limitations during a project. As a result, we could itemize five common features, which are presented within the processes; (1) flexibility, (2) constant communication of the workgroup, (3) use of UML, (4) the inclusion of the end-user and (5) some documentation.
Sulfoxaflor Applied via Drip Irrigation Effectively Controls Cotton Aphid (Aphis gossypii Glover)
Authors: Jiang Hui , Wu Hanxiang , Chen Jianjun , Tian Yongqing , Zhang Zhixiang , Xu Hanhong
Published: October 2019

Aphis gossypii Glover is a major pest of cotton and can severely affect cotton yield and lint quality. In this study, the efficacy of sulfoxaflor applied via drip irrigation and foliar spray on controlling cotton aphids was evaluated in 2016 and 2017 in Xinjiang, China. The distribution of sulfoxaflor in cotton roots, stems, leaves, and aphids, as well as its effects on two natural enemies of aphids, were also investigated. Results showed that sulfoxaflor applied through drip irrigation mainly concentrated in leaves and provided effective control of cotton aphids for 40 days, compared to 20 days when applied through foliar spray. Furthermore, drip application resulted in much lower sulfoxaflor concentrations in aphids than foliar spray. As a result, ladybird beetle and lacewing populations were higher in drip applied plants than in foliar sprayed plants. Additionally, the cost of drip irrigation was lower than foliar spray as cotton plants are commonly irrigated via drip irrigation in Xinjiang. Our results showed that application of sulfoxaflor through drip irrigation is an effective way of controlling cotton aphids in Xinjiang due to a prolonged control period, safety to two natural enemies, and lower cost of application.
Novel One-Pot Synthesis of Methyl 4-Hydroxy-2-thioxo-1,2-dihydroquinoline-3-carboxylate: Synthetic and Crystallographic Studies
Authors: Kovalenko Sergiy M. , Drushlyak Oleksandr G. , Konovalova Irina S. , Mariutsa Illia O. , Kravchenko Dmitry V. , Ivachtchenko Alexandre V. , Mitkin Oleg D.
Published: October 2019

A new suitable method of synthesis of methyl 4-hydroxy-2-thioxo-1,2-dihydroquinoline-3-carboxylate by condensation of methyl 2-isothiocyanatobenzoate and methyl malonate is described. The structure of the compound both and by-product methyl 2-(methoxycarbonothioylamino)benzoate was confirmed by means of elemental analysis, 1H NMR, 13C NMR, LC/MS and single crystal X-ray diffraction. UV/Vis and IR spectra of compounds are described. The presence of a strong intramolecular hydrogen bond between the hydroxy group and the carbonyl oxygen atom of the ester group in methyl 4-hydroxy-2-thioxo-1,2-dihydroquinoline-3-carboxylate is shown. The crystal structure of product was stabilized through intermolecular hydrogen bonds.
Mechanical and Acoustic Emission Behavior of Gangue Concrete under Uniaxial Compression
Authors: Xiao Meng , Ju Feng , Ning Pai , Li Kaiyuan
Published: October 2019

The application of gangue concrete can be an effective method to solve the massive gangue heap and shortage of raw materials of concrete by replacing the gravel and river sand with crushed gangue. An Acoustic emission (AE) is one of the non-destructive testing methods that can be used for damage detection of the gangue concrete structure. However, there are obvious mechanics differences between gangue and gravel/river sand, so the previous analysis methods of AE signal for concrete structure detection, mainly applied to ordinary concrete, are not suitable for gangue concrete. Based on this, the physical and mechanical characteristics of coal gangue were studied, and the uniaxial compressive test, along with AE monitoring of gangue concrete, was conducted in this paper. The differences in AE behavior between gangue concrete and ordinary concrete were also analyzed. The mechanical test result shows that the compressive strength of gangue concrete can reach 35–40 MPa. Comparing with ordinary concrete, gangue concrete has larger initial porosity and abrupt rupture. Additionally, the accumulative energy growth rate of gangue concrete has two peak values before the peak load, while ordinary concrete only has one. This difference can be used to forecast damage of gangue concrete structure by AE technology. This paper shows the possibility of making concrete by coal gangue, and the possibility of identifying its damage degree with the use of acoustic emission technology.
Utilization of Several Industrial Wastes as Raw Material for Calcium Sulfoaluminate Cement
Authors: Julphunthong Phongthorn , Joyklad Panuwat
Published: October 2019

The aim of this research was to study the production of calcium sulfoaluminate (CSA) cement from several industrial waste materials including with marble dust waste, flue gas desulfurization gypsum, ceramics dust waste, and napier grass ash. The chemical composition, microstructure, and phase composition of raw materials were examined using energy dispersive X-ray fluorescence (EDXRF), scanning electron microscopy (SEM), and X-ray diffraction (XRD), respectively. All raw wastes were analyzed using their chemical composition to assign proportion for raw mixture. The raw mixture is calcined at controlled calcination temperatures ranging from 1200 °C to 1300 °C for 30 min. Subsequently, with analysis, their phase composition is calculated by the Rietveld refinement technique. The results suggested that phase composition of clinker calcined at 1250 °C shows the closest composition when compared to target phases, and was selected to prepare CSA cement. The FTIR analysis was performed to study the hydration processes of CSA cement. The Ordinary Portland cement (OPC) based with adding CSA cement between 20 wt.% and 40 wt.% were investigated for the effect of CSA cement fraction on water requirement, setting times and compressive strength. The results showed that rapid setting and high early strength can be achieved by the addition of 20–40 wt.% CSA cement to OPC.
Optimization of Geometry Parameters of Inkjet-Printed Silver Nanoparticle Traces on PDMS Substrates Using Response Surface Methodology
Authors: Abu-Khalaf Jumana , Al-Ghussain Loiy , Nadi Ahmad , Saraireh Razan , Rabayah Abdulrahman , Altarazi Safwan , Al-Halhouli Ala’aldeen
Published: October 2019

Inkjet printing is an emerging technology with key advantages that make it suitable for the fabrication of stretchable circuits. Specifically, this process is cost-effective and less complex compared to conventional fabrication technologies. Inkjet printing has several process and geometry parameters that significantly affect the electromechanical properties of the printed circuits. This study aims to optimize the geometry parameters of inkjet-printed silver nanoparticle traces on plasma-treated polydimethylsiloxane (PDMS) substrates. The optimization process was conducted for two printed shapes, namely straight line and horseshoe patterns. The examined input factors for the straight line traces were: the number of inkjet-printed layers and line width. On the other hand, the number of cycles and amplitude were the examined input parameters for the horseshoe shape. First, the optimal number of layers and line width were found from the straight line analysis and subsequently were used in the optimization of the horseshoe pattern parameters. The optimization of the input parameters was carried out using the response surface methodology (RSM), where the objective of the optimization was to maximize the breakdown strain of the traces while maximizing the gauge factor and minimizing the ink cost. The results indicate that a 1.78 mm line width and one layer are the optimal geometry parameters for the straight line traces, while for the horseshoe pattern, the optimal parameters are one layer, a line width of 1.78 mm, amplitude of 4 mm and one cycle. The optimal straight line was designed to sustain up to 10% strain while the horseshoe pattern was designed to sustain up to 15% strain.
Microscopic Analysis of Steel Corrosion Products in Seawater and Sea-Sand Concrete
Authors: Wang Gang , Wu Qing , Li Xue-Zhong , Xu Jun , Xu Yao , Shi Wen-Hao , Wang Shi-Lin
Published: October 2019

In this paper, the types, formation time, structural morphology, and influence of steel corrosion products in seawater and sea-sand concrete were studied, and the intermediate and final products of steel corrosion under different conditions were determined. The corrosion products of steel in these concrete specimens under two curing methods were studied separately by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). Due to the presence of a large amount of chloride ions in the concrete, the rust layer on the surface of a steel bar contained many intermediates, such as lepidocrocite (γ–FeOOH) and aka-ganeite (β–FeOOH). Under wet/dry cycles, with the addition and loss of moisture in concrete, various corrosion products were also dynamically converted into each other. In the specimens immersed in seawater for a long time, the intermediates of corrosion were lepidocrocite (γ–FeOOH) and aka-ganeite (β–FeOOH), which were substituted for oxygen as the new depolarizers of cathode reduction reaction, and consumed themselves to ensure smooth corrosion.
Forming of Dynamic Microstructure of Flexible Polymer
Authors: Weng Yung-Jin
Published: October 2019

This study focuses on the forming of dynamic microstructure of flexible polymer. The dynamic tensile control microstructure of the polymer mold, along with the gasbag, was used to exert pressure to achieve forming. This study simulated the dynamic control of the flexible mold, and proposed four mechanical models of material viscoelastic response for modeling and evaluation. MATLAB software was used to calculate the imprint prediction calculation theory construction according to the imprint result of curved surface and asymmetric imprint forming. This study designed and developed a gasbag-assisted dynamic forming system, and tested the proposed system for verification. The test results showed that the mechanical stability, curved surface, and asymmetric imprint prediction calculation of the mechanical model of the viscoelastic response of flexible mold material, as proposed in this study, can display the geometric features of the imprinted microstructure. The dynamic mold microstructure control process can accurately transfer a bifacial microstructure and construct the confidence interval for transfer printing forming.
Oxytree Pruned Biomass Torrefaction: Process Kinetics
Authors: Świechowski Kacper , Stegenta-Dąbrowska Sylwia , Liszewski Marek , Bąbelewski Przemysław , Koziel Jacek A. , Białowiec Andrzej
Published: October 2019

Oxytree is a fast-growing energy crop with C4 photosynthesis. In this research, for the first time, the torrefaction kinetic parameters of pruned Oxytree biomass (Paulownia clon in Vitro 112) were determined. The influence of the Oxytree cultivation method and soil class on the kinetic parameters of the torrefaction was also investigated. Oxytree pruned biomass from a first-year plantation was subjected to torrefaction within temperature range from 200 to 300 °C and under anaerobic conditions in the laboratory-scale batch reactor. The mass loss was measured continuously during the process. The relative mass loss increased from 1.22% to 19.56% with the increase of the process temperature. The first-order constant rate reaction (k) values increased from 1.26 × 10−5 s−1 to 7.69 × 10−5 s−1 with the increase in temperature. The average activation energy for the pruned biomass of Oxytree torrefaction was 36.5 kJ∙mol−1. Statistical analysis showed no significant (p < 0.05) effect of the Oxytree cultivation method and soil class on the k value. The results of this research could be useful for the valorization of energy crops such as Oxytree and optimization of waste-to-carbon and waste-to-energy processes.
Fabrication and Characterization of Silk Fibroin/Curcumin Sustained-Release Film
Authors: Zhang Xiaoning , Chen Zhenyu , Bao Hong , Liang Jianwei , Xu Shui , Cheng Guotao , Zhu Yong
Published: October 2019

In the present work, a sustained-release film composed of silk fibroin (SF), curcumin (Cur), glutaraldehyde (GA), and glycerol (Gly) was prepared successfully for wound dressings. Features relevant to wound dressings of SF/Gly/GA/Cur film were assessed. Physical and chemical properties of the fabricated materials were also characterized. The results showed that the prepared SF/Gly/GA/Cur film demonstrated a good sustained-release performance, flexibility, and gas permeability. In addition, it was found that the prepared SF/Gly/GA/Cur film possessed the capability to effectively inhibit the growth of bacteria and prevent bacterial penetration with a suitable water vapor transmission rate. Furthermore, the prepared composite film was non-cytotoxic, which makes it an ideal material for wound dressings.