bluuebunny/crossref_metadata_2025 huggingface dataset:
At the heart of inflammation: Unravelling cardiac resident macrophage biology
Authors: Liao Yingnan , Zhu Liyuan
Published: September 2024

Cardiovascular disease remains one of the leading causes of death globally. Recent advancements in sequencing technologies have led to the identification of a unique population of macrophages within the heart, termed cardiac resident macrophages (CRMs), which exhibit self‐renewal capabilities and play crucial roles in regulating cardiac homeostasis, inflammation, as well as injury and repair processes. This literature review aims to elucidate the origin and phenotypic characteristics of CRMs, comprehensively outline their contributions to cardiac homeostasis and further summarize their functional roles and molecular mechanisms implicated in the onset and progression of cardiovascular diseases. These insights are poised to pave the way for novel therapeutic strategies centred on targeted interventions based on the distinctive properties of resident macrophages.
Highly expressed B3GALT5‐AS1 contributes to gastric cancer progression by recruiting WDR5 to mediate B3GALT5 and regulating β‐catenin/ZEB1 axis
Authors: Feng Wei , Tang Yelan , Jing Rongrong , Ju Shaoqing , Zong Wei
Published: September 2024

Long non‐coding RNAs (lncRNAs) play an important role in the progression of gastric cancer (GC), but its specific regulatory mechanism remains to be further studied. We previously identified that lncRNA B3GALT5‐AS1 was upregulated in GC serum. Here, we investigated the functions and molecular mechanisms of B3GALT5‐AS1 in GC tumorigenesis. qRT‐PCR was used to detect B3GALT5‐AS1 expression in GC. EdU, CCK‐8, and colony assays were utilized to assess the proliferation ability of B3GAL5‐AS1, and transwell, tube formation assay were used to assess the invasion and metastasis ability. Mechanically, FISH and nuclear plasmolysis PCR identified the subcellular localization of B3GALT5‐AS1. RIP and CHIP assays were used to analyse the regulation of B3GALT5‐AS1 and B3GALT5. We observed that B3GALT5‐AS1 was highly expressed in GC, and silencing B3GALT5‐AS1 could inhibit the proliferation, invasion, and migratory capacities of GC. Additionally, B3GALT5‐AS1 was bound to WDR5 and modulated the expression of B3GALT5 via regulating the ZEB1/β‐catenin pathway. High‐expressed B3AGLT5‐AS1 promoted GC tumorigenesis and regulated B3GALT5 expression via recruiting WDR5. Our study is expected to provide a new idea for clinical diagnosis and treatment.
Viral eradication reduces all‐cause mortality in patients with chronic hepatitis C virus infection who had received direct‐acting antiviral therapy
Authors: Tada Toshifumi , Kurosaki Masayuki , Toyoda Hidenori , Tamaki Nobuharu , Yasui Yutaka , Nakamura Shinichiro , Mori Nami , Tsuji Keiji , Ochi Hironori , Akahane Takehiro , Kobashi Haruhiko , Fujii Hideki , Marusawa Hiroyuki , Kondo Masahiko , Urawa Naohito , Yoshida Hideo , Uchida Yasushi , Morita Atsuhiro , Hasebe Chitomi , Mitsuda Akeri , Ogawa Chikara , Narita Ryoichi , Kubotsu Yoshihito , Matsushita Tomomichi , Shigeno Masaya , Okamoto Eisuke , Okada Kazuhiko , Kasai Toyotaka , Ishii Toru , Nonogi Michiko , Yasuda Satoshi , Koshiyama Yuichi , Kumada Takashi , Izumi Namiki
Published: September 2024

Background and Aims

The impact of hepatitis C virus (HCV) eradication via direct‐acting antiviral (DAA) therapy on overall mortality, particularly non‐liver‐related mortality, is understudied.

Methods

We recruited 4180 patients with chronic HCV infection who achieved sustained virological response (SVR) (HCV eradication) through DAA therapy (n = 2501, SVR group) or who did not receive antiviral therapy (n = 1679, non‐SVR group); 1236 from each group were chosen using propensity score matching. Causes of death and all‐cause mortality, including non‐liver‐related diseases, were investigated.

Results

Of the 4180 patients, 592 died during the follow‐up period. In the SVR group, the mortality rates from liver‐related and non‐liver‐related diseases were 16.5% and 83.5%, respectively. Compared to the non‐SVR group, mortality rates from liver‐related and non‐liver‐related diseases were 50.1% and 49.9%, respectively (p < .001). In non‐cirrhotic patients, multivariable analysis revealed that SVR was an independent factor associated with both liver‐related (hazard ratio [HR], .251; 95% confidence interval [CI], .092–.686) and non‐liver‐related (HR, .641; 95% CI, .415–.990) mortalities. In cirrhotic patients, multivariable analysis revealed that SVR remained an independent factor significantly associated with liver‐related mortality (HR, .151; 95% CI, .081–.279). In propensity score‐matched patients, the eradication of HCV (SVR group) decreased both liver‐related (p < .001) and non‐liver‐related mortality (p = .008) rates compared to persistent HCV infection (non‐SVR group).

Conclusions

The elimination of HCV via DAA therapy reduced not only liver‐related mortality but also non‐liver‐related mortality in patients with chronic HCV.
Equity, diversity, and inclusion in entrustable professional activities based assessment
Authors: Hennus Marije P. , Chen H. Carrie
Published: September 2024

Recognizing the value of Entrustable Professional Activities, Hennus and Chen argue that deliberate and conscious efforts at implementation are pivotal to addressing and mitigating the risks of racism and bias.
VirB11, a traffic ATPase, mediated flagella assembly and type IV pilus morphogenesis to control the motility and virulence of Xanthomonas albilineans
Authors: Li Meilin , Xiong Liya , Chen Wenhan , Li YiSha , Khan Abdullah , Powell Charles A. , Chen Baoshan , Zhang Muqing
Published: September 2024

Xanthomonas albilineans (Xal) is a gram‐negative bacterial pathogen responsible for developing sugarcane leaf scald disease, which engenders significant economic losses within the sugarcane industry. In the current study, homologous recombination exchange was carried out to induce mutations within the virB/D4‐like type IV secretion system (T4SS) genes of Xal. The results revealed that the virB11‐deletion mutant (ΔvirB11) exhibited a loss in swimming and twitching motility. Application of transmission electron microscopy analysis further demonstrated that the ΔvirB11 failed to develop flagella formation and type IV pilus morphology and exhibited reduced swarming behaviour and virulence. However, these alterations had no discernible impact on bacterial growth. Comparative transcriptome analysis between the wild‐type Xal JG43 and the deletion‐mutant ΔvirB11 revealed 123 differentially expressed genes (DEGs), of which 28 and 10 DEGs were notably associated with flagellar assembly and chemotaxis, respectively. In light of these findings, we postulate that virB11 plays an indispensable role in regulating the processes related to motility and chemotaxis in Xal.
The association between salivary pepsin and gastroesophageal reflux disease: A meta‐analysis
Authors: Han Fei , Li Xiangyu , Song Zhaoxiang , Xie Jinlin , Wang Nan , Yao Jianning
Published: September 2024

Background and Purpose

The definitive diagnosis of gastroesophageal reflux disease (GERD) often requires invasive investigations like upper gastrointestinal endoscopy or reflux monitoring. We aimed to explore the relationship between salivary pepsin and GERD and its value as a non‐invasive diagnostic tool.

Methods

Databases (PubMed, Web of Science, Cochran Library, and EMBASE) were searched from their inception to January 22, 2024 to explore the correlation of salivary pepsin with GERD. The meta‐analysis data retrieved were summarized, including the salivary pepsin concentration, sensitivity of diagnosis (SEN), specificity of diagnosis (SPE), negative likelihood ratio, positive likelihood ratio, diagnostic odds ratio, and receiver operating characteristic (ROC) curve.

Results

The meta‐analysis comparing salivary pepsin concentration in two groups (proven GERD and non‐GERD) with 18 studies revealed that the proven GERD group had higher salivary pepsin concentration than the non‐GERD group (SMD = 1.74 [95% CI 1.14–2.34]). The meta‐analysis of salivary pepsin diagnostic value for proven GERD incorporated 23 studies. The results showed pooled SEN (0.73 [95% CI 0.66–0.80]), SPE (0.72 [95% CI 0.65–0.78]), positive likelihood ratio (2.61 [95% CI 2.02–3.39]), negative likelihood ratio (0.37 [95% CI 0.28–0.50]), diagnostic odds ratio (7.03 [95% CI 4.24–11.66]) and area under the SROC curve (0.79 [95% CI 0.75–0.82]).

Conclusion

GERD patients presented a higher salivary pepsin concentration. Salivary pepsin is both sensitive and specific in identifying GERD, making it a promising non‐invasive marker for diagnosis.
How Does the Stress in the Fixation Device Change during Different Stages of Bone Healing in the Treatment of Fractures? A Finite Element Study of External Fixation for Tibial Fractures
Authors: Jia Xuehai , Shen Changyong , Luo Bin , Yang Yi , Zhang Kerui , Deng Yi , Wen Jun , Ma Litai
Published: September 2024

Background

Although the specific relationship between the stress changes in the external fixator during tibial fracture treatment and the bone healing process remains unclear, it is believed that stress variations in the external fixator scaffold can, to a certain extent, reflect the progress of tibial healing.

Objective

This study aims to propose a non‐invasive method for assessing the degree of fracture healing by monitoring the changes in stress transmission, the locations of stress‐sensitive points, and displacement in the external fixator‐tibia system during the healing process of tibial fractures.

Methods

In this study, finite element models of tibial fractures at various healing stages were developed. Physiological conditions, including axial, torsional, and bending loads on the tibia, were simulated to evaluate stress and strain within the external scaffold‐tibia system under normal physiological loading conditions.

Results

The results indicate variations in the stress distribution between the external fixator and the tibia during different stages of healing. In the early phase of fracture healing, the external fixator plays a crucial role as the primary load‐bearing unit under all three loading conditions. As the fracture healing progresses, the stress on the tibia gradually increases, concentrating on the medial part of the tibia under axial and torsional loading, and at the upper and lower ends, as well as the central part of the anterior and posterior tibia during bending loading. The stress at the callus gradually increases, while micro‐movements decrease. The stress within the external bracket gradually decreases, with a tendency for the connecting rod to transfer stress towards the screws. Throughout the fracture healing process, the location of maximum stress in the external fixator remains unchanged. Under axial and torsional loading, the maximum stress is located at the intersection of the lowest screw and the bone cortex, while under bending loading, it is at the intersection of the second screw and the connecting rod.

Conclusion

During the bone healing process, stress is transferred between the external fixation frame and the bone. As bone healing advances, the stress on the connecting rods and screws of the external fixation frame decreases, and the amplitude of stress changes diminishes. When complete and robust fusion is achieved, stress variations stabilize, and the location of maximum stress on the external fixation frame remains unchanged. The intersections of the lowest screw and the bone cortex, as well as the second screw and the connecting rod, can serve as sensitive points for monitoring the degree of bone healing.
Liver Transplantation for Nijmegen Breakage Syndrome With Hepatic Malignancy and Hepatopulmonary Syndrome After Bone Marrow Transplantation: A Case Report
Authors: Şal Oğuzhan , Erbey Fatih , Armutlu Ayşe , Karasu Gülsün , Demir Barış , Kızılkan Nuray Uslu , Akbulut Akın , Kanmaz Turan , Alim Altan
Published: September 2024

Background

Nijmegen breakage syndrome (NBS) is an autosomal recessive DNA repair disorder that manifests through increased genomic instability, malignancy, and cellular and humoral immunodeficiencies. The prognosis for NBS patients is poor due to their increased susceptibility to fatal infections and lymphoproliferative malignancies. Currently, there is no specific treatment for NBS, though allogeneic hematopoietic stem cell transplantation (HSCT) has been performed and documented as case series to demonstrate the utility of transplantation.

Methods

A 14‐year‐old girl with NBS and haploidentical HSCT from her older brother due to recurrent lung infection was referred for liver transplantation (LT) due to liver cirrhosis, hepatopulmonary syndrome (HPS), and suspicion of liver malignancy. It was decided to perform LT using the living donor who had previously donated for HSCT.

Results

Living donor left lobe LT was successfully performed from her brother. The patient experienced no complications in the early postoperative period and was discharged on the seventh postoperative day. Pathological examination of extracted liver has shown “intermediate cell carcinoma” in two foci. After 1 year LT, the patient has had an uneventful course in terms of LT complications and infection, with minimal immunosuppression.

Conclusions

NBS patients have an increased prevalence of malignancies, including primary hepatic malignancy, but most are managed medically or with limited resections. Transplantation in these patients can be curative for hepatic malignancy with a favorable safety profile.
Putting the puzzle together: the relationship between floral characters and pollinator morphology determines pollination mode in the fig–fig wasp mutualism
Authors: Castro‐Cárdenas N. , Martén‐Rodríguez S. , Vázquez‐Santana S. , Cornejo‐Tenorio G. , Navarrete‐Segueda A. , Ibarra‐Manríquez G.
Published: September 2024

The diversification of angiosperms has largely been attributed to adaptive radiation of their pollination and mating systems, which are relevant drivers of the macroevolution processes. The fig (Ficus, Moraceae) and fig wasp (Agaonidae, Hymenoptera) interaction is an example of obligate mutualism. Passive and active pollination modes have been associated with morphological traits in both partners. However, more information is required to assess the relationship between floral traits and pollination modes, particularly in Neotropical Ficus species.

This study evaluates the morphological traits of figs and fig wasps regarding pollination modes in species belonging to Neotropical Ficus sections (three species each of Americanae and Pharmacosycea). Pollination mode was identified by floral morphology, anther/ovule ratio, and specialized structures fig wasps use for pollen transport (pollen pocket and coxal combs).

Fig species in sect. Americanae are actively pollinated because pistillate flowers form a synstigma, present anther/ovule ratios <0.11, and their pollinator Pegoscapus fig wasps have pollen pockets and coxal combs. In contrast, species in sect. Pharmacosycea have free pistillate flowers, with anther/ovule ratios >0.27; they are pollinated by Tetrapus wasps, which lack specialized structures to carry pollen. Each species of Ficus was associated with a single morphospecies of fig wasp.

The results support previous contributions that consider reciprocal morphological traits between fig species and their pollinating wasps as evidence of a close co‐evolutionary history.
Comprehensive analysis of hairpin small RNAs involved in resistance and pathogenesis during wheat–Puccinia triticina interactions
Authors: Afreen Uzma , Pandey Anjali , Jha Shailendra Kumar , Kumar Manish , Mukhopadhyay Kunal
Published: September 2024

Wheat leaf rust, caused by the fungus Puccinia triticina (Pt), severely affects the grain quality and quantity of bread wheat (Triticum aestivum L.). Hairpin small(s)RNAs, like micro(mi)RNAs and their variants [including isomiRNAs (isomiRs) and microRNA‐like RNAs (milRNAs)], along with their corresponding target genes, bestow leaf rust disease resistance, development and progression from both interacting species. However, the regulatory networks remain inadequately understood. Thirteen differentially expressed novel miRNAs, including two isomiRs and three milRNAs were discerned from induced reads of wheat sRNA libraries, and a further 5,393 and 1,275 candidate target genes were predicted in wheat and Pt, respectively. Functional annotation divulged that wheat‐originated miRNAs/isomiRs were involved in resistance, while Pt‐derived milRNAs imparted pathogenesis. The identified milRNAs‐ Tae‐Pt‐milR5, Tae‐Pt‐milR12, and Tae‐Pt‐milR14b and their cleavage sites on Pt target gene MEP5 were confirmed through degradome library screening, suggesting cross‐kingdom translocation of Pt virulent genes in wheat host. Co‐expression analysis of miRNAs/isomiRs‐target genes provided insights into combating leaf rust disease, while co‐expression analysis of milRNAs‐target gene pairs reflected the extent of pathogenicity exerted by Pt with varied expression levels at the analyzed time points. The analysis pinpointed leaf rust‐responsive candidate hairpin sRNAs‐ Tae‐miR8, Tae‐Pt‐miR12, Tae‐Pt‐miR14a, and Tae‐Pt‐miR14b in wheat and Tae‐Pt‐milR12 in Pt. This study provides new insights into the hairpin sRNAs involved in the resistance and pathogenesis of wheat and Pt, respectively. Furthermore, crucial hairpin sRNAs and their promising targets for future biotechnological interventions to augment stress resilience have been identified.
A phylogeographic analysis of the North American medicinal leech, Macrobdella decora (Say, 1824)
Authors: Kennedy Nat , Kvist Sebastian , Oceguera‐Figueroa Alejandro , Phillips Anna J. , Stacey Donald F. , de Carle Danielle
Published: September 2024

In spite of their important roles in many ecosystems, data regarding population structure and biogeographic patterns of leeches are scarce. To begin to address this knowledge gap, we herein perform a phylogeographic analysis of the North American medicinal leech, Macrobdella decora (Say, 1824). A total of 224 M. decora specimens were collected from 35 localities across large swaths of USA and Canada and covering most of the known range of the species. Using four loci (mitochondrial cytochrome c oxidase subunit I [COI] and NADH dehydrogenase I [ND1], as well as nuclear 18S rRNA [18S] and 28S rRNA [28S]), we construct phylogenetic trees using several optimality criteria and superimpose geographic patterns onto the trees in order to tease out any potential structure among the populations. Rather surprisingly, given the large geographic range of the species and abundance of potential geographic barriers to gene flow, the analyses showed a conspicuous lack of structure among the different populations of M. decora. However, an AMOVA did show statistically significant differences between the genetic variation within populations and between populations (COI: FST = 0.65412, p < .00001; ND1: FST = 0.69245, p < .00001), which was largely driven by only 6 out of the 35 populations, and indicated a potential barrier for dispersal across the Appalachian Mountains. Finally, a Mantel test showed a weak, but significant, correlation between geographic distance and genetic distance (COI: r = 0.209, p = .027; ND1: r = 0.1289, p = .030); however, this correlation was primarily driven by a single locality. The overall weak structure suggests that M. decora is panmictic throughout its range, and we discuss this in light of previous population level studies in both bloodfeeding and non‐bloodfeeding species, concluding that the lack of structure in M. decora might be due to its high capacity for dispersal via hosts.
Abordaje geriátrico integral : escalas de uso frecuente en geriatría
Authors:   Pontificia Universidad Javeriana,   Pontificia Universidad Javeriana
Published: September 2024

El personal de salud que brinda atención a un adulto mayor requiere herramientas adaptadas y actualizadas para entender todas las dimensiones del cuidado. En este sentido, Abordaje geriátrico integral ofrece a sus lectores un conjunto de escalas para la valoración geriátrica integral (VGI) en diferentes niveles asistenciales, así como la experiencia de profesionales que, de forma didáctica, comparten sus conocimientos. Este libro busca estar a la mano de los profesionales de la salud para facilitar la interpretación de la información de la historia clínica del adulto mayor y para enriquecerla, a partir de cuatro dimensiones de la valoración geriátrica integral. También abarca campos que vienen en creciente desarrollo de la geriatría, como la ortogeriatría, la oncogeriatría y los cuidados paliativos. A través de tablas y figuras presentadas con una metodología didáctica para facilitar la interpretación y toma de decisiones, este libro marca la diferencia en la atención diaria al adulto mayor, con el objetivo de mejorar la calidad de vida de las personas mayores, la de sus familias y la comunidad, en consonancia con lo propuesto por la Organización Mundial de la Salud (OMS) en la Década del Envejecimiento Saludable (2021-2030).
In Vivo Quantification of Ascending Thoracic Aortic Aneurysm Wall Stretch Using MRI: Relationship to Repair Threshold Diameter and Ex Vivo Wall Failure Behavior
Authors: Dong Huiming , Haraldsson Henrik , Leach Joseph , Zhou Ang , Ballweber Megan , Zhu Chengcheng , Xuan Yue , Wang Zhongjie , Hope Michael , Epstein Frederick H. , Ge Liang , Saloner David , Tseng Elaine , Mitsouras Dimitrios
Published: September 2024

Ascending thoracic aortic aneurysms (aTAAs) can lead to life-threatening dissection and rupture. Recent studies have highlighted aTAA mechanical properties as relevant factors associated with progression. The aim of this study was to quantify in vivo aortic wall stretch in healthy participants and aTAA patients using displacement encoding with stimulated echoes (DENSE) magnetic resonance imaging. Moreover, aTAA wall stretch between surgical and nonsurgical patients was investigated. Finally, DENSE measurements were compared to reference-standard mechanical testing on aTAA specimens from surgical repairs. In total, 18 subjects were recruited, six healthy participants and 12 aTAA patients, for this prospective study. Electrocardiogram-gated DENSE imaging was performed to measure systole–diastole wall stretch, as well as the ratio of aTAA stretch to unaffected descending thoracic aorta stretch. Free-breathing and breath-hold DENSE protocols were used. Uniaxial tensile testing-measured indices were correlated to DENSE measurements in five harvested specimens. in vivo aortic wall stretch was significantly lower in aTAA compared to healthy subjects (1.75±1.44% versus 5.28±1.92%, respectively, P = 0.0004). There was no correlation between stretch and maximum aTAA diameter (P = 0.56). The ratio of aTAA to unaffected thoracic aorta wall stretch was significantly lower in surgical candidates compared to nonsurgical candidates (0.993±0.011 versus 1.017±0.016, respectively, P = 0.0442). Finally, in vivo aTAA wall stretch correlated to wall failure stress and peak modulus of the intima (P = 0.017 and P = 0.034, respectively), while the stretch ratio correlated to whole-wall thickness failure stretch and stress (P = 0.013 and P = 0.040, respectively). Aortic DENSE has the potential to assess differences in aTAA mechanical properties and progressions.
Supplemental Material: The onset of Paleo-Pacific subduction: Key evidence from the Kaishantun Accretionary Complex, northeastern Eurasia
Authors: Wang Hong-Yan , Zhou Jian-Bo , al. et
Published: September 2024

Supplemental Text S1: Analytical Methods. Table S1: LA-ICP-MS zircon U-Pb data for sedimentary rocks from the Kaishantun area. Table S2: Trace element (ppm) analyses of zircons within the sedimentary rocks from the Kaishantun Accretionary Complex. Table S3: Lu-Hf isotopic data of zircons from the Kaishantun Accretionary Complex in Northeast China.
Comment on “tracking rule” and generalization of special relativity
Authors: Lambare Justo Pastor
Published: September 2024

In a recent article in this journal (Canadian Journal of Physics 102, 43 (2024)), Alexander L. Kholmetskii, Oleg V. Missevitch, and Tolga Yarman criticized our views on the tracking rule and issued some inaccurate statements related to them. We clarify those statements explicating their content and analyze the meaning of the tracking rule as a valid principle to generalize special relativity.
Agritourism as a strategy of remote island’s development: the case study of Kasos
Authors: Vythoulka Anastasia , Kaliakmanis Fotis , Delegou Ekaterini , Giannikouris Antonis , Kampanis Nikolaos , Alexandrakis Georgios , Moropoulou Antonia , Chlorokostas Stavros , Magkoufis Efthymios , Kontopoulos Christos , Charalampopoulou Vasiliki
Published: September 2024

In rural, depopulated areas, agritourism, as a development model, could serve the goal of non- exclusively seasonal tourism while creating additional economic opportunities for the locals who are mainly engaged in agriculture. Kasos is an isolated island of the Dodecanese, Greece, preserving interesting elements of the authentic agricultural practices and architecture like terraces serving the island’s agricultural cultivation, mills and mitata, small stone- built creameries.

Kasos served as a case study of AEI program (Sustainable Development of Less Developed Regions and Isolated Areas by Creating New Touristic Resources and Products through Analysis, Documentation and Modelling of Cultural Assets using Innovative ICT Applications, project code:T2EDK- 01278). For thιs island that lies within the wider region of Rhodes, it is possible with the appropriate development of new touristic resources and products through the advancement of the unique architectural, cultural and environmental assets to orient towards them special parts of the touristic flow from Rhodes, creating new touristic activity by increasing the residence beyond the daily or short time tourism, as it stands for now.

For the analysis of the study area, research in local archives and communication with the municipality and local stakeholders was conducted. Thus, both the special features of the island and the needs of the local community were identified, organized, classified and presented in GIS maps. The implementation of a GIS for Kasos Island aims to provide a comprehensive representation of the different aspects of the island, providing useful information for policy-making and the development of strategic cultural and agrotourism plans to the local authorities and stakeholders.

According to the integrated plan for Kasos by AEI program, the island could preserve its special rural identity while revealing its cultural and environmental assets and attracting external economies within an agritourism model. In this work, we present AEI’s integrated plan for Kasos that refers to the island’s agricultural character, to the existing trail network as well as to the restoration and adaptive reuse of important architectural assets that could serve to the overall revealing of Kasos history and cultural reserve.
MRI Predicts Residual Disease and Outcomes in Watch-and-Wait Patients with Rectal Cancer
Authors: Williams Hannah , Omer Dana M. , Thompson Hannah M. , Lin Sabrina T. , Verheij Floris S. , Miranda Joao , Yuval Jonathan B. , Buckley James , Marco Michael R. , Qin Li-Xuan , Dombroski David A. , Kedar Rajendra , Oto Aytekin , Korngold Elena , Veniero Joseph C. , Gandhi Sunil , Krishnaraj Arun , Jagtiani Minal , Ohanian Kirk , Vu Dan , Hope Thomas A. , Lee Sonia , Wasnik Ashish P. , Madhuripan Nikhil , Gollub Marc J. , Garcia-Aguilar Julio ,   on behalf of the OPRA Consortium, Patil Sujata , Kim Jin K. , Lee Meghan , Dunne Richard F. , Marcet Jorge , Cataldo Peter , Polite Blase , Herzig Daniel O. , Liska David , Oommen Samuel , Friel Charles M. , Ternent Charles , Coveler Andrew L. , Hunt Steven , Gregory Anita , Varma Madhulika G. , Bello Brian L. , Carmichael Joseph C. , Krauss John , Gleisner Ana , Paty Philip B. , Weiser Martin R. , Nash Garrett M. , Pappou Emmanouil , Guillem José G. , Temple Larissa , Wei Iris H. , Widmar Maria , Segal Neil H. , Cercek Andrea , Yaeger Rona , Smith J. Joshua , Goodman Karyn A. , Wu Abraham J. , Saltz Leonard B.
Published: September 2024

Restaging MRI can stratify patients treated with total neoadjuvant therapy into clinical response categories predictive of organ preservation, local regrowth, and survival.
Feeding intolerance after pediatric cardiac surgery is associated with dysbiosis, barrier dysfunction, and reduced short-chain fatty acids
Authors: Owens Jacob , Qiu Haowen , Knoblich Cole , Gerjevic Lisa , Izard Jacques , Xu Linda , Lee Junghyae , Kollala Sai Sundeep , Murry Daryl J. , Riethoven Jean Jack , Davidson Jesse A. , Singh Amar B. , Ibrahimiye Ali , Ortmann Laura , Salomon Jeffrey D.
Published: September 2024

Feeding intolerance contributes to postoperative morbidity following pediatric cardiac surgery. The intestinal microbiome and milieu play a vital role in gut function. Short-chain fatty acids are gut and cardioprotective metabolites produced by commensal bacteria and help maintain appropriate barrier function. Depletion of these metabolites and barrier dysfunction contribute to postoperative feeding intolerance following cardiac surgery. Identifying mechanistic targets to improve the intestinal milieu with the goal of improved nutrition and clinical outcomes is critical.
Extracellular Vesicle–Packaged ACSL4 Induces Hepatocyte Senescence to Promote Hepatocellular Carcinoma Progression
Authors: Hou Pei-pei , Zheng Chong-ming , Wu Si-hong , Liu Xi-xiao , Xiang Guang-xin , Cai Wei-yang , Chen Gang , Lou Yong-liang
Published: September 2024

Extracellular vesicles (EV) derived from cancer cells are crucial mediators of intercellular communication during tumor progression. The cargo in tumor-derived EVs that facilitates the establishment of a tumor-supportive microenvironment could serve as a therapeutic target to improve cancer treatment. Here, we demonstrated that hepatocellular carcinoma (HCC) cells secreted the acyl-CoA synthetase long-chain family member 4 (ACSL4) in large EVs (lEV) to modulate tumor–microenvironment interactions that promote HCC progression. HCC-derived lEV ACSL4 increased the intracellular abundance of polyunsaturated fatty acid–containing lipids and remodeled the lipid profile to potentiate lipid peroxidation in peritumoral hepatocytes, resulting in hepatocyte senescence accompanied by the senescence-associated secretory phenotype. Depletion of senescent hepatocytes by senolytic treatment suppressed tumor progression. In HCC cells, SREBP2-mediated transcriptional activation upregulated ACSL4 expression, and Akt-mediated phosphorylation of ACSL4 induced its packaging into lEVs by augmenting its interaction with Annexin A2. This study identified the critical regulatory function of ACSL4 secreted from HCC cells in inducing lipid remodeling and senescence in hepatocytes to support HCC progression, suggesting that targeting lEV ACSL4 is a potential therapeutic strategy for HCC.

Significance: Peritumoral hepatocyte senescence mediated by ACSL4 secreted from hepatocellular carcinoma cells in extracellular vesicles promotes tumor progression through a senescence secretome and represents a therapeutic target in liver cancer.
Neural Circuitries between the Brain and Peripheral Solid Tumors
Authors: Chen Xiang , Geng Yuli , Wei Guanxin , He Danzeng , Lv Jialong , Wen Wenhao , Xiang Fan , Tao Kaixiong , Wu Chuanqing
Published: September 2024

The recent discovery of the pivotal role of the central nervous system in controlling tumor initiation and progression has opened a new field of research. Increasing evidence suggests a bidirectional interaction between the brain and tumors. The brain influences the biological behavior of tumor cells through complex neural networks involving the peripheral nervous system, the endocrine system, and the immune system, whereas tumors can establish local autonomic and sensory neural networks to transmit signals into the central nervous system, thereby affecting brain activity. This review aims to summarize the latest research in brain–tumor cross-talk, exploring neural circuitries between the brain and various peripheral solid tumors, analyzing the roles in tumor development and the related molecular mediators and pathologic mechanisms, and highlighting the critical impact on the understanding of cancer biology. Enhanced understanding of reciprocal communication between the brain and tumors will establish a solid theoretical basis for further research and could open avenues for repurposing psychiatric interventions in cancer treatment.
Supplementary Table S9 from Long-Term Risks of Cardiovascular Death among Older Patients with Major Hematological Malignancies: A Population-Based Cohort Study from SEER Database
Authors: Zhang Hanqing , He Minghao , Zhang Peng , Gao Yang , Ouyang Ling , He Xianjun , Han Na , Zhang Jinfeng , Guan Mengshan , Feng Yueqi , Li Yonghua
Published: September 2024

Supplementary Table S9. Heart disease-related Standardized Mortality Ratios in older patient with different types of hematological malignancies based on years after diagnosis.
Supplementary Table S6 from Long-Term Risks of Cardiovascular Death among Older Patients with Major Hematological Malignancies: A Population-Based Cohort Study from SEER Database
Authors: Zhang Hanqing , He Minghao , Zhang Peng , Gao Yang , Ouyang Ling , He Xianjun , Han Na , Zhang Jinfeng , Guan Mengshan , Feng Yueqi , Li Yonghua
Published: September 2024

Supplementary Table S6. Standardized Mortality Ratios and Absolute Excess Risks for Specific CVD death with Radiotherapy and Ann Arbor stage.
Supplementary Figure S8 from Long-Term Risks of Cardiovascular Death among Older Patients with Major Hematological Malignancies: A Population-Based Cohort Study from SEER Database
Authors: Zhang Hanqing , He Minghao , Zhang Peng , Gao Yang , Ouyang Ling , He Xianjun , Han Na , Zhang Jinfeng , Guan Mengshan , Feng Yueqi , Li Yonghua
Published: September 2024

Supplementary Figure S8. The distribution of causes of deaths among older patients with hematological malignancies based on different malignancy types. For each panel, we show the distribution of four causes of death among different types of hematological malignancy base on years after diagnosis: acute myeloid leukemia, chronic lymphocytic leukemia, chronic myeloid leukemia, multiple myeloma, Hodgkin lymphoma, non-Hodgkin lymphoma, and acute lymphocytic leukemia
Supplementary Figure S6 from Long-Term Risks of Cardiovascular Death among Older Patients with Major Hematological Malignancies: A Population-Based Cohort Study from SEER Database
Authors: Zhang Hanqing , He Minghao , Zhang Peng , Gao Yang , Ouyang Ling , He Xianjun , Han Na , Zhang Jinfeng , Guan Mengshan , Feng Yueqi , Li Yonghua
Published: September 2024

Supplementary Figure S6. The distribution of causes of deaths among older patients with hematological malignancies (2004-2018). For each panel, we show the distribution based on different causes of death, the distribution of all four causes of deaths (a), the distribution of all causes of non-malignancies deaths (b), the distribution of all causes of CVD-related deaths (c). The time interval does not include the upper limit. Abbreviations: CVD, Cardiovascular Disease
Supplementary Table S7 from Evaluation of the Immune Response within the Tumor Microenvironment in African American and Non-Hispanic White Patients with Non–Small Cell Lung Cancer
Authors: Trendowski Matthew R. , Watza Donovan , Lusk Christine M. , Lonardo Fulvio , Ratliff Valerie , Wenzlaff Angela S. , Mamdani Hirva , Neslund-Dudas Christine , Boerner Julie L. , Schwartz Ann G. , Gibson Heather M.
Published: September 2024

Immune-centric Pathway Analysis of Genetic Predictors of PD-L1 Expression and TLS Status in NSCLC Cases.
Data from Geographic Access to Fertility Counseling among Adolescent and Young Adult Women with Cancer in North Carolina
Authors: Rodriguez-Ormaza Nidia , Anderson Chelsea , Baggett Christopher D. , Delamater Paul L. , Troester Melissa A. , Wheeler Stephanie B. , Wardell Alexis C. , Deal Allison M. , Smitherman Andrew , Mersereau Jennifer , Baker Valerie L. , Nichols Hazel B.
Published: September 2024

AbstractBackground:
Fertility counseling is recommended for adolescent and young adult women facing gonadotoxic cancer therapy. However, fertility care is subspecialized medical care offered at a limited number of institutions, making geographic access a potential barrier to guideline-concordant care. We assessed the relationship between geographic access and receipt of fertility counseling among adolescent and young adult women with cancer.
Methods:
Using data from the North Carolina Central Cancer Registry, we identified women diagnosed with lymphoma, gynecologic cancer, or breast cancer at ages 15 to 39 years during 2004 to 2015. Eligible women were invited to complete an online survey on various topics, including fertility counseling. Geographic access was measured, using geocoded addresses, as vehicular travel time from residence to the nearest fertility clinic available at diagnosis. Multivariable regression models were used to examine the association between travel time and receipt of fertility counseling by provider type: health care provider versus fertility specialist.
Results:
Analyses included 380 women. The median travel time to a fertility clinic was 31 (IQR: 17–71) minutes. Overall, 75% received fertility counseling from a health care provider and 16% by a fertility specialist. Women who lived ≥30 minutes from a clinic were 13% less likely to receive fertility counseling by a health care provider (prevalence ratio: 0.87; 95% confidence interval, 0.75–1.00) and 49% less likely to receive counseling by a fertility specialist (prevalence ratio: 0.51; 95% confidence interval, 0.28–0.93).
Conclusions:
Women who lived further away from fertility clinics were less likely to receive fertility counseling.
Impact:
Interventions to improve access to fertility counseling should include strategies to alleviate the burden of geographic access.
Supplementary Table S5 from Streamlined Intraoperative Brain Tumor Classification and Molecular Subtyping in Stereotactic Biopsies Using Stimulated Raman Histology and Deep Learning
Authors: Reinecke David , Ruess Daniel , Meissner Anna-Katharina , Fürtjes Gina , von Spreckelsen Niklas , Ion-Margineanu Adrian , Khalid Florian , Blau Tobias , Stehle Thomas , Al-Shugri Abdulkader , Büttner Reinhard , Goldbrunner Roland , Ruge Maximilian I. , Neuschmelting Volker
Published: September 2024

Supplementary Table S5. The second model was used to predict the class of each acquired entity from small biopsy tissue samples. These predictions were compared to the frozen section diagnosis. The level of accuracy depended on the final diagnosis and the predefined model label assignment to the respective subclasses. Furthermore, the sample size in square millimeters was gathered to compare the available specimen samples between SRH and the frozen section diagnosis.
Supplementary Table S4 from Streamlined Intraoperative Brain Tumor Classification and Molecular Subtyping in Stereotactic Biopsies Using Stimulated Raman Histology and Deep Learning
Authors: Reinecke David , Ruess Daniel , Meissner Anna-Katharina , Fürtjes Gina , von Spreckelsen Niklas , Ion-Margineanu Adrian , Khalid Florian , Blau Tobias , Stehle Thomas , Al-Shugri Abdulkader , Büttner Reinhard , Goldbrunner Roland , Ruge Maximilian I. , Neuschmelting Volker
Published: September 2024

Supplementary Table S4. The third model was developed to classify adult-type diffuse gliomas into molecular subclasses, e.g., IDH-mutant and 1p/19q-non-codeleted, IDH-mutant and 1p/19q-codeleted, and IDH-wildtype. The corresponding class predictions are compared to the final neuropathological diagnosis.
Supplementary Table S4 from Maximizing Treatment Opportunities: Assessing Protocol Waivers’ Impact on Safety and Outcome in the Drug Rediscovery Protocol
Authors: van Berge Henegouwen Jade M. , Zeverijn Laurien J. , Geurts Birgit S. , Hoes Louisa R. , van der Wijngaart Hanneke , van der Noort Vincent , Huitema Alwin D.R. , de Vos Filip Y.F. , Grünberg Katrien , Bloemendal Haiko J. , Verheul Henk M.W. , Voest Emile E. , Gelderblom Hans
Published: September 2024

An overview of waivers granted for patients that were non-evaluable for the study’s primary endpoint
Supplementary Figure S6 from A Phase II Study of Potentiation of Pembrolizumab with Binimetinib and Bevacizumab in Refractory Microsatellite-Stable Colorectal Cancer
Authors: Lentz Robert W. , Friedrich Tyler J. , Blatchford Patrick J. , Jordan Kimberly R. , Pitts Todd M. , Robinson Hannah R. , Davis S. Lindsey , Kim Sunnie S. , Leal Alexis D. , Lee Mathew R. , Waring Meredith R.N. , Martin Anne C. , Dominguez Adrian T.A. , Bagby Stacey M. , Hartman Sarah J. , Eckhardt S. Gail , Messersmith Wells A. , Lieu Christopher H.
Published: September 2024

Supplementary Figure S6. Correlative peripheral blood immune cell characterization at baseline.
Supplementary Tables and Figures 1 from Ibrutinib in Combination with R-GemOx in Patients with Relapsed or Refractory Diffuse Large B-cell Lymphoma of Nongerminal Center B-cell–like Type: Phase II Clinical Trial of the Spanish GELTAMO Group
Authors: Rey-Búa Beatriz , Grande Carlos , Sánchez Blanco José J. , Abrisqueta Pau , Gutiérrez Antonio , Ramírez Páyer Ángel , Giné Eva , Zeberio Etxetxipia Izaskun , Terol Maria J. , de la Cruz Vicente Fátima , Andreu Rafel , Ramirez Maria J. , de la Fuente Adolfo , Viguria Maria C. , Peñarrubia María J. , Jiménez-Ubieto Ana , Montes-Moreno Santiago , López-Guillermo Armando , Caballero María D. , Martín García-Sancho Alejandro
Published: September 2024

Supplementary Tables and Figures
Supplementary Table 2 from A Phase I/II Study of Valemetostat (DS-3201b), an EZH1/2 Inhibitor, in Combination with Irinotecan in Patients with Recurrent Small-Cell Lung Cancer
Authors: Choudhury Noura J. , Lai W. Victoria , Makhnin Alex , Heller Glenn , Eng Juliana , Li Bob , Preeshagul Isabel , Santini Fernando C. , Offin Michael , Ng Kenneth , Paik Paul , Larsen Christina , Ginsberg Michelle S. , Lau Yvonne , Zhang Xinyuan , Baine Marina K. , Rekhtman Natasha , Rudin Charles M.
Published: September 2024

Pharmacokinetics Data
Supplementary Figure 1 from A Phase I/II Study of Valemetostat (DS-3201b), an EZH1/2 Inhibitor, in Combination with Irinotecan in Patients with Recurrent Small-Cell Lung Cancer
Authors: Choudhury Noura J. , Lai W. Victoria , Makhnin Alex , Heller Glenn , Eng Juliana , Li Bob , Preeshagul Isabel , Santini Fernando C. , Offin Michael , Ng Kenneth , Paik Paul , Larsen Christina , Ginsberg Michelle S. , Lau Yvonne , Zhang Xinyuan , Baine Marina K. , Rekhtman Natasha , Rudin Charles M.
Published: September 2024

Pharmacokinetics
Supplementary Figure S5 from Inhibition of Aberrantly Overexpressed Polo-like Kinase 4 Is a Potential Effective Treatment for DNA Damage Repair–Deficient Uterine Leiomyosarcoma
Authors: Lee Horace H.Y. , Chow Kin Long , Wong Ho Shing , Chong Tsz Yan , Wong Alice S.T. , Cheng Grace H.W. , Ko Jasmine M.K. , Siu Hoi Cheong , Yeung Maximus C.F. , Huen Michael S.Y. , Tse Ka Yu , Bray Mark R. , Mak Tak Wah , Leung Suet Yi , Ip Philip P.C.
Published: September 2024

Supplementary Figure S5. Oncoplot of key genes responsible for DNA repair among sarcoma cell lines.
Supplementary Figure S2 from Inhibition of Aberrantly Overexpressed Polo-like Kinase 4 Is a Potential Effective Treatment for DNA Damage Repair–Deficient Uterine Leiomyosarcoma
Authors: Lee Horace H.Y. , Chow Kin Long , Wong Ho Shing , Chong Tsz Yan , Wong Alice S.T. , Cheng Grace H.W. , Ko Jasmine M.K. , Siu Hoi Cheong , Yeung Maximus C.F. , Huen Michael S.Y. , Tse Ka Yu , Bray Mark R. , Mak Tak Wah , Leung Suet Yi , Ip Philip P.C.
Published: September 2024

Supplementary Figure S2. DNA content and centrosome number quantification in myogenic sarcoma cell lines after CFI-400945 treatment.
Supplemental Figure 4 from Microsatellite Instability, Tumor Mutational Burden, and Response to Immune Checkpoint Blockade in Patients with Prostate Cancer
Authors: Lenis Andrew T. , Ravichandran Vignesh , Brown Samantha , Alam Syed M. , Katims Andrew , Truong Hong , Reisz Peter A. , Vasselman Samantha , Nweji Barbara , Autio Karen A. , Morris Michael J. , Slovin Susan F. , Rathkopf Dana , Danila Daniel , Woo Sungmin , Vargas Hebert A. , Laudone Vincent P. , Ehdaie Behfar , Reuter Victor , Arcila Maria , Berger Michael F. , Viale Agnes , Scher Howard I. , Schultz Nikolaus , Gopalan Anuradha , Donoghue Mark T.A. , Ostrovnaya Irina , Stopsack Konrad H. , Solit David B. , Abida Wassim
Published: September 2024

Supplemental Figure 4
Supplementary Figure S8 from Combination Targeted Therapy with Pembrolizumab and Lenvatinib in Progressive, Radioiodine-Refractory Differentiated Thyroid Cancers
Authors: French Jena D. , Haugen Bryan R. , Worden Francis P. , Bowles Daniel W. , Gianoukakis Andrew G. , Konda Bhavana , Dadu Ramona , Sherman Eric J. , McCue Shaylene , Foster Nathan R. , Nikiforov Yuri E. , Farias Ticiana D.J. , Norman Paul J. , Wirth Lori J.
Published: September 2024

Increased HED and HLA diversity are not associated with improved PFS
Supplemental Figure 2 from Microsatellite Instability, Tumor Mutational Burden, and Response to Immune Checkpoint Blockade in Patients with Prostate Cancer
Authors: Lenis Andrew T. , Ravichandran Vignesh , Brown Samantha , Alam Syed M. , Katims Andrew , Truong Hong , Reisz Peter A. , Vasselman Samantha , Nweji Barbara , Autio Karen A. , Morris Michael J. , Slovin Susan F. , Rathkopf Dana , Danila Daniel , Woo Sungmin , Vargas Hebert A. , Laudone Vincent P. , Ehdaie Behfar , Reuter Victor , Arcila Maria , Berger Michael F. , Viale Agnes , Scher Howard I. , Schultz Nikolaus , Gopalan Anuradha , Donoghue Mark T.A. , Ostrovnaya Irina , Stopsack Konrad H. , Solit David B. , Abida Wassim
Published: September 2024

Supplemental Figure 2
Supplementary Figure S5 from Combination Targeted Therapy with Pembrolizumab and Lenvatinib in Progressive, Radioiodine-Refractory Differentiated Thyroid Cancers
Authors: French Jena D. , Haugen Bryan R. , Worden Francis P. , Bowles Daniel W. , Gianoukakis Andrew G. , Konda Bhavana , Dadu Ramona , Sherman Eric J. , McCue Shaylene , Foster Nathan R. , Nikiforov Yuri E. , Farias Ticiana D.J. , Norman Paul J. , Wirth Lori J.
Published: September 2024

Peripheral T cell subsets and response
Supplementary Figure S2 from Combination Targeted Therapy with Pembrolizumab and Lenvatinib in Progressive, Radioiodine-Refractory Differentiated Thyroid Cancers
Authors: French Jena D. , Haugen Bryan R. , Worden Francis P. , Bowles Daniel W. , Gianoukakis Andrew G. , Konda Bhavana , Dadu Ramona , Sherman Eric J. , McCue Shaylene , Foster Nathan R. , Nikiforov Yuri E. , Farias Ticiana D.J. , Norman Paul J. , Wirth Lori J.
Published: September 2024

T cell and myeloid cell staining in DTC tumors
Supplementary Figure S1 from Combination Targeted Therapy with Pembrolizumab and Lenvatinib in Progressive, Radioiodine-Refractory Differentiated Thyroid Cancers
Authors: French Jena D. , Haugen Bryan R. , Worden Francis P. , Bowles Daniel W. , Gianoukakis Andrew G. , Konda Bhavana , Dadu Ramona , Sherman Eric J. , McCue Shaylene , Foster Nathan R. , Nikiforov Yuri E. , Farias Ticiana D.J. , Norman Paul J. , Wirth Lori J.
Published: September 2024

Consort diagram
Supplementary Figure S9 from Detecting Small Cell Transformation in Patients with Advanced EGFR Mutant Lung Adenocarcinoma through Epigenomic cfDNA Profiling
Authors: El Zarif Talal , Meador Catherine B. , Qiu Xintao , Seo Ji-Heui , Davidsohn Matthew P. , Savignano Hunter , Lakshminarayanan Gitanjali , McClure Heather M. , Canniff John , Fortunato Brad , Li Rong , Banwait Mandeep K. , Semaan Karl , Eid Marc , Long Henry , Hung Yin P. , Mahadevan Navin R. , Barbie David A. , Oser Matthew G. , Piotrowska Zofia , Choueiri Toni K. , Baca Sylvan C. , Hata Aaron N. , Freedman Matthew L. , Berchuck Jacob E.
Published: September 2024

H3K27ac signal at select genes in representative cfDNA samples from a healthy cancer-free control, a patient with metastatic EGFRm LUAD, and a patient with metastatic EGFRm tSCLC.