bluuebunny/crossref_metadata_2025 huggingface dataset:
Conducting and Magnetic Hybrid Polypyrrole/Nickel Composites and Their Application in Magnetorheology
Authors: Jurča Marek , Vilčáková Jarmila , Kazantseva Natalia E. , Munteanu Andrei , Munteanu Lenka , Sedlačík Michal , Stejskal Jaroslav , Trchová Miroslava , Prokeš Jan
Published: December 2023

Hybrid organic/inorganic conducting and magnetic composites of core–shell type have been prepared by in-situ coating of nickel microparticles with polypyrrole. Three series of syntheses have been made. In the first, pyrrole was oxidised with ammonium peroxydisulfate in water in the presence of various amounts of nickel and the composites contained up to 83 wt% of this metal. The second series used 0.1 M sulfuric acid as a reaction medium. Finally, the composites with polypyrrole nanotubes were prepared in water in the presence of structure-guiding methyl orange dye. The nanotubes have always been accompanied by the globular morphology. FTIR and Raman spectroscopies confirmed the formation of polypyrrole. The resistivity of composite powders of the order of tens to hundreds Ω cm was monitored as a function of pressure up to 10 MPa. The resistivity of composites slightly increased with increasing content of nickel. This apparent paradox is explained by the coating of nickel particles with polypyrrole, which prevents their contact and subsequent generation of metallic conducting pathways. Electrical properties were practically independent of the way of composite preparation or nickel content and were controlled by the polypyrrole phase. On the contrary, magnetic properties were determined exclusively by nickel content. The composites were used as a solid phase to prepare a magnetorheological fluid. The test showed better performance when compared with a different nickel system reported earlier.
Bis(triethoxysilyl)ethane (BTESE)–Organosilica Membranes for H2O/DMF Separation in Reverse Osmosis (RO): Evaluation and Correlation of Subnanopores via Nanopermporometry (NPP), Modified Gas Translation (mGT) and RO Performance
Authors: Mohd Ibrahim Suhaina Mohd , Sawamura Ken-ichi , Mishina Kengo , Yu Xin , Salak Feridoun , Miyata Shigeru , Moriyama Norihiro , Nagasawa Hiroki , Kanezashi Masakoto , Tsuru Toshinori
Published: December 2023

A 40 cm length Bis(triethoxysilyl)ethane (BTESE) membrane having different pore sizes was successfully prepared by changing the number of coating times for gas permeation (GP) and organic solvent reverse osmosis (OSRO) separation study. It was found that BTESE-6 membranes prepared through six-time coating consisted of small-sized pores in the range 0.56 to 0.64 nm estimated using modified Gas Translation (mGT) method and 0.59 to 0.67 nm estimated by nanopermporometry (NPP) method, respectively. These membranes demonstrated a high DMF rejection, RDMF > 95% with total flux, Jv total > 5 kg m−2 h−1 at operating condition feed pressure, Pf: 8 MPa; feed temperature, Tf : 50 °C; and feed flowrate, Qf : 30 mL/min; and they exhibited a high degree selectivity of He/SF6 in the range of ~ 260–3400 at a permeation temperature 200 °C. On the other hand, the larger pore sizes of the BTESE-4 membranes (pore size estimates > 0.76 nm to 1.02 nm) exhibited low DMF rejection and a low degree selectivity of He/SF6 around ~30% and 25, respectively, at the same operating condition as BTESE-6. Both GT and NPP methods can be considered as an indicator of the measurement membrane pore size. From this study, it was found that He and SF6 gases can be some of the potential predictors for water and DMF permeance. Furthermore, by comparing our OSRO membrane with other PV membranes for DMF/H2O separation, our BTESE-6 membranes still exhibited high flux in the range of 3–6 kg m−2 h−1 with a separation factor H2O/DMF in the range of 80–120.
Metabolomic Changes in Plasma of Preterminal Stage of Rhesus Nonhuman Primates Exposed to Lethal Dose of Radiation
Authors: Carpenter Alana D. , Fatanmi Oluseyi O. , Wise Stephen Y. , Petrus Sarah A. , Tyburski John B. , Cheema Amrita K. , Singh Vijay K.
Published: December 2023

Ionizing radiation exposure is known to induce molecular and cellular injury, inflicting a cascade of potentially catastrophic events leading to tissue and organ damage. Metabolomic analysis allows for the identification and quantification of small molecules downstream of genomic changes induced by radiation exposure. We aimed to characterize metabolomic changes that underscore the prefinal stage of lethally irradiated rhesus nonhuman primates (NHPs). Peripheral blood was drawn at baseline, post-exposure, as well as at the preterminal stage in NHPs (immediately prior to death in moribund NHPs) that did not survive exposure with 7.2 Gy total-body radiation (LD70/60). Herein, we analyzed global metabolomic changes using ultra-performance liquid chromatography (UPLC) quadrupole time-of-flight mass spectrometry (QTOF-MS) in plasma samples of NHPs collected at various timepoints in relation to irradiation. The overall goal was to identify metabolic shifts present immediately prior to death. Our findings showed radiation induced significant time-dependent metabolic perturbations when compared to pre-irradiation profiles, particularly in glycerophospholipid metabolism and steroid hormone biosynthesis and metabolism pathways. These findings provide valuable insights for identifying biomarkers for lethality, which may be helpful for triage during a mass casualty scenario.
Multi-Objective Optimization of a Long-Stroke Moving-Iron Proportional Solenoid Actuator
Authors: Liu Peng , Ouyang Yuwen , Quan Wenwen
Published: December 2023

In this study, the performance of a long-stroke moving-iron proportional solenoid actuator (MPSA) was improved by combining numerical simulations and experiments. A finite element model of the MPSA was developed; its maximum and mean relative absolute errors of electromagnetic force were 4.3% and 2.3%, respectively, under typical work conditions. Seven design parameters including the cone angle, cone length, depth of the inner hole of the coil skeleton, cone width of the armature, inner cone diameter, and initial position of the moving-iron core were selected for developing the model, and the coefficient of the variation in electromagnetic force, nominal acceleration, 95% of the maximum stable output electromagnetic force, and corresponding response time were used as the performance indicators. The constraint relation between each performance indicator and the influence of each design parameter on the performance indicators were revealed using the uniform Latin hypercube experiment design, correlation analysis, and the main effect analysis method. A multi-objective optimization mathematical model of the MPSA was developed by combining traditional surrogate and machine learning models. The Pareto solution set was obtained using the nondominated sorting genetic algorithm II (NSGA-II), and three decision schemes with different attitudes were determined using the Hurwicz multi-criteria decision-making method. The results showed that a strong contradiction exists among the 95% of the maximum stable output electromagnetic force and its corresponding response time and the coefficient of the variation in electromagnetic force. The cone angle considerably influenced the performance indicators. Compared with the initial design, the coefficient of the variation in electromagnetic force was reduced by 54.08% for the positive decision, the corresponding response time was shortened by 15.65% for the critical decision, and the corresponding acceleration was enhanced by 10.32% for the passive decision. Thus, the overall performance of the long-stroke MPSA effectively improved.
Recent Developments in Fabrication Methods and Measurement Schemes for Optically Pumped Magnetic Gradiometers: A Comprehensive Review
Authors: Dong Haifeng , Ye Hangfei , Hu Min , Ma Zongmin
Published: December 2023

Optically pumped gradiometers have long been utilized in measurement in the International Geomagnetic Reference Field (IGRF). With advancements in technologies such as laser diodes and microfabrication, integrated gradiometers with compact sizes have become available, enabling improvements in magnetoencephalography and fetal magnetocardiography within shielded spaces. Moreover, there is a growing interest in the potential of achieving biomagnetic source detection without shielding. This review focuses on recent developments in optically pumped magnetic field gradiometers, including various fabrication methods and measurement schemes. The strengths and weaknesses of different types of optically pumped gradiometers are also analyzed.
A Hybrid Energy-Efficient, Area-Efficient, Low-Complexity Switching Scheme in SAR ADC for Biosensor Applications
Authors: Hu Yunfeng , Chen Chaoyi , Huang Qingming , Hu Lexing , Tang Bin , Hu Mengsi , Yuan Bingbing , Wu Zhaohui , Li Bin
Published: December 2023

A hybrid energy-efficient, area-efficient, low-complexity switching scheme in SAR ADC for biosensor applications is proposed. This scheme is a combination of the monotonic technique, the MSB capacitor-splitting technique, and a new switching method. The MSB capacitor-splitting technique, as well as the reference voltage Vaq allow for more options for reference voltage conversion, resulting in higher area savings and higher energy efficiency. In a capacitor array, the circuit performs unilateral switching during all comparisons except for the second and last two comparisons, reducing the difficulty in designing the drive circuit. The proposed switching scheme saves 98.4% of the switching energy and reduces the number of unit capacitors by 87.5% compared to a conventional scheme. Furthermore, the SAR ADC employs low-noise and low-power dynamic comparators utilizing multi-clock control, low-sampling error-sampling switches based on the bootstrap technique, and dynamic SAR logic. The simulation results demonstrated that the proposed SAR ADC achieves 61.51 dB SNDR, 79.21 dB SFDR and consumes 0.278 μW of power in a 180 nm process with a 1 V power supply, a full swing input signal frequency of 23.33 kHz, and a sampling rate of 100 kS/s.
Quantitative Evaluation of Paleocene Reservoir Diagenetic Facies by Logging in Lishui West Sag, East China Sea Basin
Authors: Teng Ji , Fu Qiang , Qin Jun , Ma Wenrui , Zhang Guanyu , Feng Kailong
Published: December 2023

Exploration in the Lishui West Sag of the East China Sea Basin is limited by the scarcity of offshore drilling sites, and the prediction of deep, high-quality reservoirs is challenging using only geophysical methods. This study introduces a quantitative approach to diagenetic facies division in individual wells based on logging data, providing a new method and perspective for the prediction of deep, high-quality reservoirs. We employed comprehensive data from core, logging, thin-section casting, cathodoluminescence, scanning electron microscopy, and X-ray diffraction analyses from five wells to study the petrology, physical properties, diagenetic types and strength, and diagenetic minerals of the Paleocene sandstone reservoirs in the Lishui West Sag. Apparent compaction rate, apparent cementation rate, and other quantitative characterization parameters were used to calculate the comprehensive diagenetic coefficient (Cg), and the diagenetic facies were divided into compaction, cementation, and dissolution facies. A logging calculation model for the comprehensive diagenetic coefficient (Cg) and a quantitative identification method for diagenetic facies in individual well reservoirs were established through a fitting analysis between Cg and logging curve parameters. Continuous quantitative identification of vertical diagenetic facies in the five wells in the study area showed that the high-quality reservoirs in wells L1, L2, and L3 within the L1 gas field are characterized by extensive development of dissolution facies, while wells L4 and L5 are dominated by compaction and cementation facies, with poor reservoir properties and no industrial gas flow output. The results demonstrate the reliability of the model method. The establishment of this quantitative characterization method for diagenetic facies using logging data provides guidance for the prediction of favorable reservoirs.
Enhancing Solid-Phase Extraction of Tamoxifen and Its Metabolites from Human Plasma Using MOF-Integrated Polyacrylonitrile Composites: A Study on CuBTC and ZIF-8 Efficacy
Authors: Flores-Hernandez Domingo R. , Leija Gutiérrez Héctor Manuel , Hernandez-Hernandez Jose A. , Sánchez-Fernández José Antonio , Bonilla-Rios Jaime
Published: December 2023

This study investigates electrospun fibers of metal-organic frameworks (MOFs), particularly CuBTC and ZIF-8, in polyacrylonitrile (PAN) for the solid-phase extraction (SPE) of Tamoxifen (TAM) and its metabolites (NDTAM, ENDO, and 4OHT) from human blood plasma. The focus is on the isolation, pre-concentration, and extraction of the analytes, aiming to provide a more accessible and affordable breast cancer patient-monitoring technology. The unique physicochemical properties of MOFs, such as high porosity and surface area, combined with PAN’s stability and low density, are leveraged to improve SPE efficiency. The study meticulously examines the interactions of these MOFs with the analytes under various conditions, including elution solvents and protein precipitators. Results reveal that ZIF-8/PAN composites outperform CuBTC/PAN and PAN alone, especially when methanol is used as the protein precipitator. This superior performance is attributed to the physicochemical compatibility between the analytes’ properties, like solubility and polarity, and the MOFs’ structural features, including pore flexibility, active site availability, surface polarity, and surface area. The findings underscore MOFs’ potential in SPE applications and provide valuable insights into the selectivity and sensitivity of different MOFs towards specific analytes, advancing more efficient targeted extraction methods in biomedical analysis.
Design Polyaniline/α-Zirconium Phosphate Composites for Achieving Self-Healing Anti-Corrosion of Carbon Steel
Authors: Lv Ziqi , Ren Kai , Liu Tao , Zhao Yunyan , Zhang Zhonghua , Li Guicun
Published: December 2023

The rupture of a micro/nano container can trigger the release of repair agents and provides the coating with a self-healing and anti-corrosion effect. However, the defect and inhomogeneity of the coating, produced by the rupture of the micro/nano container, may weaken its anti-corrosion performance. This study reports a rare protection mechanism, which optimizes the space occupying of zirconium phosphate, and the de-doping peculiarity of polyaniline without the rupture of the micro/nano container. Polyaniline/α-zirconium phosphate composites were constructed through in situ oxidation polymerization. Repair agents were added in the form of doped acids. According to the different repair agents in polyaniline/α-zirconium phosphate composites (citric ion, tartaric ion and phytic ion), the performance and protection mechanism of the composites were researched. Polyaniline/α-zirconium phosphate coating (with phytic ion) shows an excellent self-healing anti-corrosive effect, due to the large spatial structure and abundant chelating groups of the precipitation inhibitor. Considering the anti-corrosive application, the developed polyaniline/α-zirconium phosphate composite has a far-reaching influence on marine development.
Spectromicroscopy Study of Induced Defects in Ion-Bombarded Highly Aligned Carbon Nanotubes
Authors: Tayyab Sammar , Apponi Alice , Betti Maria Grazia , Blundo Elena , Cavoto Gianluca , Frisenda Riccardo , Jiménez-Arévalo Nuria , Mariani Carlo , Pandolfi Francesco , Polimeni Antonio , Rago Ilaria , Ruocco Alessandro , Sbroscia Marco , Yadav Ravi Prakash
Published: December 2023

Highly aligned multi-wall carbon nanotubes were investigated with scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) before and after bombardment performed using noble gas ions of different masses (argon, neon and helium), in an ultra-high-vacuum (UHV) environment. Ion irradiation leads to change in morphology, deformation of the carbon (C) honeycomb lattice and different structural defects in multi-wall carbon nanotubes. One of the major effects is the production of bond distortions, as determined by micro-Raman and micro-X-ray photoelectron spectroscopy. We observe an increase in sp3 distorted bonds at higher binding energy with respect to the expected sp2 associated signal of the carbon 1s core level, and increase in dangling bonds. Furthermore, the surface damage as determined by the X-ray photoelectron spectroscopy carbon 1s core level is equivalent upon bombarding with ions of different masses, while the impact and density of defects in the lattice of the MWCNTs as determined by micro-Raman are dependent on the bombarding ion mass; heavier for helium ions, lighter for argon ions. These results on the controlled increase in sp3 distorted bonds, as created on the multi-wall carbon nanotubes, open new functionalization prospects to improve and increase atomic hydrogen uptake on ion-bombarded multi-wall carbon nanotubes.
Mechanical Strength and Conductivity of Cementitious Composites with Multiwalled Carbon Nanotubes: To Functionalize or Not?
Authors: O’Rear Edgar A. , Onthong Suthisa , Pongprayoon Thirawudh
Published: December 2023

The incorporation of carbon nanotubes into cementitious composites increases their compressive and flexural strength, as well as their electrical and thermal conductivity. Multiwalled carbon nanotubes (MWCNTs) covalently functionalized with hydroxyl and carboxyl moieties are thought to offer superior performance over bare nanotubes, based on the chemistry of cement binder and nanotubes. Anionic carboxylate can bind to cationic calcium in the hydration products, while hydroxyl groups participate in hydrogen bonding to anionic and nonionic oxygen atoms. Results in the literature for mechanical properties vary widely for both bare and modified filler, so any added benefits with functionalization are not clearly evident. This mini-review seeks to resolve the issue using an analysis of reports where direct comparisons of cementitious composites with plain and functionalized nanotubes were made at the same concentrations, with the same methods of preparation and under the same conditions of testing. A focus on observations related to the mechanisms underlying the enhancement of mechanical strength and conductivity helps to clarify the benefits of using functionalized MWCNTs.
Understanding the Effect of the Synthetic Method and Surface Chemistry on the Properties of CsPbBr3 Nanoparticles
Authors: Giancaspro Mariangela , Panniello Annamaria , Depalo Nicoletta , Comparelli Roberto , Striccoli Marinella , Curri Maria Lucia , Fanizza Elisabetta
Published: December 2023

Over the last decade, the attractive properties of CsPbBr3 nanoparticles (NPs) have driven ever-increasing progress in the development of synthetic procedures to obtain high-quality NPs at high concentrations. Understanding how the properties of NPs are influenced by the composition of the reaction mixture in combination with the specific synthetic methodology is crucial, both for further elucidating the fundamental characteristics of this class of materials and for their manufacturing towards technological applications. This work aims to shed light on this aspect by synthesizing CsPbBr3 NPs by means of two well-assessed synthetic procedures, namely, hot injection (HI) and ligand-assisted reprecipitation (LARP) in non-polar solvents, using PbBr2 and Cs2CO3 as precursors in the presence of already widely investigated ligands. The overall goal is to study and compare the properties of the NPs to understand how each synthetic method influences the NPs’ size and/or the optical properties. Reaction composition and conditions are purposely tuned towards the production of nanocubes with narrow size distribution, high emission properties, and the highest achievable concentration. As a result, the formation of bulk crystals as precipitate in LARP limits the achievement of a highly concentrated NP solution. The size of the NPs obtained by LARP seems to be poorly affected by the ligands’ nature and the excess bromide, as consequence of bromide-rich solvation agents, effectively results in NPs with excellent emission properties. In contrast, NPs synthesized by HI exhibit high reaction yield, diffusion growth-controlled size, and less striking emission properties, probably ascribed to a bromide-deficient condition.
Investigating the Protective Effects of a Citrus Flavonoid on the Retardation Morphogenesis of the Oligodendroglia-like Cell Line by Rnd2 Knockdown
Authors: Fukatsu Shoya , Miyamoto Yuki , Oka Yu , Ishibashi Maki , Shirai Remina , Ishida Yuki , Endo Shin , Katoh Hironori , Yamauchi Junji
Published: December 2023

Recent discoveries suggest links between abnormalities in cell morphogenesis in the brain and the functional deficiency of molecules controlling signal transduction in glial cells such as oligodendroglia. Rnd2 is one such molecule and one of the Rho family monomeric GTP-binding proteins. Despite the currently known functions of Rnd2, its precise roles as it relates to cell morphogenesis and disease state remain to be elucidated. First, we showed that signaling through the loss of function of the rnd2 gene affected the regulation of oligodendroglial cell-like morphological differentiation using the FBD-102b cell line, which is often utilized as a differentiation model. The knockdown of Rnd2 using the clustered regularly interspaced palindromic repeats (CRISPR)/CasRx system or RNA interference was shown to slow morphological differentiation. Second, the knockdown of Prag1 or Fyn kinase, a signaling molecule acting downstream of Rnd2, slowed differentiation. Rnd2 or Prag1 knockdown also decreased Fyn phosphorylation, which is critical for its activation and for oligodendroglial cell differentiation and myelination. Of note, hesperetin, a citrus flavonoid with protective effects on oligodendroglial cells and neurons, can recover differentiation states induced by the knockdown of Rnd2/Prag1/Fyn. Here, we showed that signaling through Rnd2/Prag1/Fyn is involved in the regulation of oligodendroglial cell-like morphological differentiation. The effects of knocking down the signaling cascade molecule can be recovered by hesperetin, highlighting an important molecular structure involved in morphological differentiation.
Focusing on Future Consequences Enhances Self-Controlled Dietary Choices
Authors: Kruse Johanna , Korb Franziska M. , Surrey Caroline , Wolfensteller Uta , Goschke Thomas , Scherbaum Stefan
Published: December 2023

Self-controlled dietary decisions, i.e., choosing a healthier food over a tastier one, are a major challenge for many people. Despite the potential profound consequences of frequent poor choices, maintaining a healthy diet proves challenging. This raises the question of how to facilitate self-controlled food decisions to promote healthier choices. The present study compared the influence of implicit and explicit information on food choices and their underlying decision processes. Participants watched two video clips as an implicit manipulation to induce different mindsets. Instructions to focus on either the short-term or long-term consequences of choices served as an explicit manipulation. Participants performed a binary food choice task, including foods with different health and taste values. The choice was made using a computer mouse, whose trajectories we used to calculate the influence of the food properties. Instruction to focus on long-term consequences compared to short-term consequences increased the number of healthy choices, reduced response times for healthy decisions, and increased the influence of health aspects during the decision-making process. The effect of video manipulation showed greater variability. While focusing on long-term consequences facilitated healthy food choices and reduced the underlying decision conflict, the current mindset appeared to have a minor influence.
Evaluation of Olive Leaf Phenolic Compounds’ Gastrointestinal Stability Based on Co-Administration and Microencapsulation with Non-Digestible Carbohydrates
Authors: Duque-Soto Carmen , Leyva-Jiménez Francisco Javier , Quirantes-Piné Rosa , López-Bascón María Asunción , Lozano-Sánchez Jesús , Borrás-Linares Isabel
Published: December 2023

The large generation of olive by-products has motivated their revalorization into high-added-value products. In this regard, olive leaves pose as an interesting source of bioactive compounds, due to their phenolic content with commonly known antioxidant, anti-inflammatory, and immunomodulatory properties, with potential application in non-communicable diseases. However, their effectiveness and applicability into functional foods is limited by their instability under gastrointestinal conditions. Thus, the development of protective formulations is essential. In this study, the spray-drying encapsulation of a phenolic-rich olive leaf extract with inulin as the encapsulating agent was optimized. Then, the behavior of the free extract under gastrointestinal conditions, its co-administration with the encapsulating agent, and the optimized microencapsulated formulation were studied through an in vitro gastrointestinal digestion process following the INFOGEST protocol. Digestion of the free extract resulted in the degradation of most compounds, whereas this was minimized in the co-administration of the non-encapsulated extract with the encapsulating agent. This protective effect, related to its interaction with inulin, was similar to the microencapsulated formulation. Thus, both approaches, co-administration and microencapsulation with inulin, could be promising strategies for the improvement of the stability of these anti-inflammatory and immunomodulatory compounds under gastrointestinal conditions, enhancing their beneficial effect.
Comparative Evaluation of a Low-Carbohydrate Diet and a Mediterranean Diet in Overweight/Obese Patients with Type 2 Diabetes Mellitus: A 16-Week Intervention Study
Authors: Currenti Walter , Losavio Francesca , Quiete Stefano , Alanazi Amer M. , Messina Giovanni , Polito Rita , Ciolli Fabiana , Zappalà Raffaela Simona , Galvano Fabio , Cincione Raffaele Ivan
Published: December 2023

Introduction: The worldwide prevalence of type 2 diabetes mellitus (T2DM) and obesity has been steadily increasing over the past four decades, with projections indicating a significant rise in the number of affected individuals by 2045. Therapeutic interventions in T2DM aim to control blood glucose levels and reduce the risk of complications. Dietary and lifestyle modifications play a crucial role in the management of T2DM and obesity. While conventional medical nutritional therapy (MNT) often promotes a high-carbohydrate, low-fat Mediterranean diet as an elective treatment, low-carbohydrate diets (LCDs), specifically those restricting carbohydrate intake to less than 130 g/day, have gained popularity due to their multifaceted benefits. Scientific research supports the efficacy of LCDs in improving glycemic control, weight loss, blood pressure, lipid profiles, and overall quality of life. However, sustaining these benefits over the long term remains challenging. This trial aimed to compare the effects of a Mediterranean diet vs. a low-carbohydrate diet (carbohydrate intake < 130 g/day) on overweight/obese patients with T2DM over a 16-week period. The study will evaluate the differential effects of these diets on glycemic regulation, weight reduction, lipid profile, and cardiovascular risk factors. Methods: The study population comprises 100 overweight/obese patients with poorly controlled T2DM. Anthropometric measurements, bioimpedance analysis, and blood chemistry assessments will be conducted at baseline and after the 16-week intervention period. Both dietary interventions were hypocaloric, with a focus on maintaining a 500 kcal/day energy deficit. Results: After 16 weeks, both diets had positive effects on various parameters, including weight loss, blood pressure, glucose control, lipid profile, and renal function. However, the low-carbohydrate diet appears to result in a greater reduction in BMI, blood pressure, waist circumference, glucose levels, lipid profiles, cardiovascular risk, renal markers, and overall metabolic parameters compared to the Mediterranean diet at the 16-week follow up. Conclusions: These findings suggest that a low-carbohydrate diet may be more effective than a Mediterranean diet in promoting weight loss and improving various metabolic and cardiovascular risk factors in overweight/obese patients with T2DM. However, it is important to note that further research is needed to understand the clinical implications and long-term sustainability of these findings.
The Associations of Vitamin D with Ovarian Reserve Markers and Depression: A Narrative Literature Review
Authors: Jeon Gyun-Ho
Published: December 2023

Since the identification of vitamin D receptors in both the female reproductive tract and the central nervous system, further data have shown that vitamin D is involved in the processes of reproductive and mental health. This paper reviews current research on the associations of vitamin D with ovarian reserve markers and depression and discusses the potential role of vitamin D in their relationships. There have been numerous studies reporting that vitamin D was significantly related to ovarian reserve markers and depression in basic or clinical research, but some observational and interventional clinical studies have shown inconsistent results. Nevertheless, recent meta-analyses of interventional studies have provided promising results showing that vitamin D supplementation significantly improves ovarian reserve metrics, especially in a subgroup of women with normal or diminished ovarian reserve, and decreases depressive symptoms and risk. The demonstration of an association of vitamin D with both ovarian reserve and depression could suggest that vitamin D may be another important key in explaining female reproductive depression. Larger-scale studies in standardized settings will be needed in order to gain further insight into the role of vitamin D in female reproduction and depression.
Molecular Mechanism of Calycosin Inhibited Vascular Calcification
Authors: Zhou Zekun , Li Yi , Jiang Wei , Wang Zengli
Published: December 2023

Vascular calcification (VC) is a pathological condition frequently observed in cardiovascular diseases. Primary factors contributing to VC are osteogenic differentiation of vascular smooth muscle and hydroxyapatite deposition. Targeted autophagy (a lysosome-mediated mechanism for degradation/recycling of unnecessary cellular components) is a useful approach for inhibiting VC and promoting vascular cell health. Calycosin has been shown to alleviate atherosclerosis by enhancing macrophage autophagy, but its therapeutic effect on VC has not been demonstrated. Using an in vitro model (rat thoracic aortic smooth muscle cell line A7r5), we demonstrated effective inhibition of VC using calycosin (the primary flavonoid component of astragalus), based on the enhancement of autophagic flux. Calycosin treatment activated AMPK/mTOR signaling to induce initiation of autophagy and restored mTORC1-dependent autophagosome–lysosome fusion in late-stage autophagy by promoting soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation, thereby preventing stoppage of autophagy in calcified cells. Calycosin substantially reduced degrees of both osteogenic differentiation and calcium deposition in our VC cell model by enhancing autophagy. The present findings clarify the mechanism whereby calycosin mitigates autophagy stoppage in calcified smooth muscle cells and provide a basis for effective VC treatment via autophagy enhancement.
Comparative Clinical Study on Periodontal Health Status and Early Diagnosis of Periodontal Diseases Quantified through Clinical Periodontal Indices on a Group of Children and Adolescents with and without Cardiovascular Diseases
Authors: Chipirliu Oana , Crăciun Marian Viorel , Matei Madalina Nicoleta
Published: December 2023

It is well known that bacterial plaque is the main etiological factor that causes the appearance of periodontal diseases and carious disease. Periodontal diseases can affect children and adolescents and are manifested in the form of gingivitis, but also the early form of chronic periodontitis as well as aggressive marginal periodontitis associated with local or general factors. Early periodontitis is frequently undiagnosed by clinicians due to a lack of knowledge of the specific symptoms. Certain systemic diseases, such as cardiovascular diseases, can create favorable conditions for the appearance and progression of severe manifestations of periodontal disease; also, recent research highlights that individuals with periodontal disease present an increased risk of developing cardiovascular diseases. Children with congenital or acquired cardiovascular diseases are at increased risk for complications resulting from the growth of microorganisms in the oral cavity, presenting a risk of infective endocarditis. The specific aim was to highlight the existing differences between the periodontal health of children with cardiovascular diseases and that of children without these diseases. The analyzed group included 124 patients, represented by children and adolescents, aged between 7 and 17 years, who were divided into four subgroups depending on the presence or absence of cardiovascular diseases and periodontal disease. A specialized clinical examination was performed for each patient, and periodontal clinical parameters were quantified (plaque index, gingival bleeding index, gingival index, community periodontal index of treatment needs) and associated with the diagnosis of general condition. Patients diagnosed with periodontal disease underwent specialized treatment and were called to a control visit 3 months after treatment. Statistical analysis showed significant differences between subgroups with much higher values of clinical parameters for patients with cardiovascular disease. Also, the response to the treatment was better in the case of patients in the control subgroup without cardiovascular diseases. The present study highlighted the interaction of three factors in the progression of periodontal diseases: subgingival microbiota, immune system response and environmental factors.
Tunable Release of Calcium from Chitosan-Coated Bioglass
Authors: Uskoković Vuk , Abuna Gabriel , Hampton Joseph Ryan , Geraldeli Saulo
Published: December 2023

Bioglass presents a standard biomaterial for regeneration of hard tissues in orthopedics and dentistry. The notable osteo-inductive properties of bioglass are largely due to the release of calcium ions from it. However, this release is not easily controllable and can often be excessive, especially during the initial interaction of the biomaterial with the surrounding tissues. Consequently, this excessive release can deplete the calcium content of the bioglass, ultimately reducing its overall bioactivity. In this study, we have tested if applying biopolymer chitosan coatings of different thicknesses would be able to mitigate and regulate the calcium ion release from monodisperse bioglass nanoparticles. Calcium release was assessed for four different chitosan coating thicknesses at different time points over the period of 28 days using a fluorescence quencher. Expectedly, chitosan-coated particles released less calcium as the concentration of chitosan in the coating solution increased, presumably due to the increased thickness of the chitosan coating around the bioglass particles. The mechanism of release remained constant for each coating thickness, corresponding to anomalous, non-Fickian diffusion, but the degree of anomalousness increased with the deposition of chitosan. Zeta potential testing showed an expected increase in the positive double layer charge following the deposition of the chitosan coating due to the surface exposure of the amine groups of chitosan. Less intuitively, the zeta potential became less positive as thickness of the chitosan coating increased, attesting to the lower density of the surface charges within thicker coatings than within the thinner ones. Overall, the findings of this study demonstrate that chitosan coating efficiently prevents the early release of calcium from bioglass. This coating procedure also allows for the tuning of the calcium release kinetics by controlling the chitosan concentration in the parent solution.
Anti-Inflammatory Effects of the LK5 Herbal Complex on LPS- and IL-4/IL-13-Stimulated HaCaT Cells and a DNCB-Induced Animal Model of Atopic Dermatitis in BALB/c Mice
Authors: Kim Hyun-Jeong , Kim So-Yeon , Bae Ho Jung , Choi Yu-Yeong , An Ju-Yeon , Cho Ye Eun , Cho So-Young , Lee Su-Jung , Lee Sanghyun , Sin MinSub , Yun Young Min , Lee Jong Ryul , Park Se Jin
Published: December 2023

Atopic dermatitis (AD) is a chronic inflammatory skin disease influenced by a complex interplay of genetic and environmental factors. The activation of the JAK-STAT pathway increases the expression of inflammatory cytokines such as IL-4 and IL-13, further deteriorating AD. Therefore, for the treatment of AD, the JAK-STAT pathway is emerging as a significant target, alongside inflammatory cytokines. This study investigates the potential therapeutic effects of a novel herbal complex, LK5, composed of Scutellaria baicalensis, Liriope platyphylla, Sophora flavescens, Dictammus dasycarpus, and Phellodendron schneider, known for their anti-inflammatory and immune-modulating properties. We examined the anti-inflammatory and anti-AD effects of the LK5 herbal complex in HaCaT cells stimulated by LPS and IL-4/IL-13, as well as in a mouse model of AD induced by DNCB. In HaCaT cells stimulated with LPS or IL-4/IL-13, the LK5 herbal complex demonstrated anti-inflammatory effects by inhibiting the expression of inflammatory cytokines including TNF-α, IL-6, and IL-1β, and downregulating the phosphorylation of STAT proteins. In a murine AD-like model induced by DNCB, administration of the LK5 herbal complex significantly ameliorated clinical symptoms, including dermatitis, ear thickness, and TEWL. Histological analysis revealed a reduction in epidermal thickness and mast cell infiltration. The LK5 herbal complex also inhibited pruritus induced by compound 48/80. Furthermore, the LK5 herbal complex treatment significantly decreased the levels of inflammatory cytokines such as TSLP, IL-6, and IgE in plasma and ear tissue of AD-induced mice. These findings suggest that the LK5 herbal complex may modulate the immune response and alleviate AD symptoms by inhibiting STAT pathways.
Co-Occurrence Analysis of Citrus Root Bacterial Microbiota under Citrus Greening Disease
Authors: Park Jong-Won , Braswell W. Evan , Kunta Madhurababu
Published: December 2023

Candidatus Liberibacter asiaticus (CLas) is associated with Citrus Huanglongbing (HLB), a devastating disease in the US. Previously, we conducted a two-year-long monthly HLB survey by quantitative real-time PCR using root DNA fractions prepared from 112 field grapefruit trees grafted on sour orange rootstock. Approximately 10% of the trees remained CLas-free during the entire survey period. This study conducted 16S metagenomics using the time-series root DNA fractions, monthly prepared during twenty-four consecutive months, followed by microbial co-occurrence network analysis to investigate the microbial factors contributing to the CLas-free phenotype of the aforementioned trees. Based on the HLB status and the time when the trees were first diagnosed as CLas-positive during the survey, the samples were divided into four groups, Stage H (healthy), Stage I (early), II (mid), and III (late) samples. The 16S metagenomics data using Silva 16S database v132 revealed that HLB compromised the diversity of rhizosphere microbiota. At the phylum level, Actinobacteria and Proteobacteria were the predominant bacterial phyla, comprising >93% of total bacterial phyla, irrespective of HLB status. In addition, a temporal change in the rhizosphere microbe population was observed during a two-year-long survey, from which we confirmed that some bacterial families differently responded to HLB disease status. The clustering of the bacterial co-occurrence network data revealed the presence of a subnetwork composed of Streptomycetaceae and bacterial families with plant growth-promoting activity in Stage H and III samples. These data implicated that the Streptomycetaceae subnetwork may act as a functional unit against HLB.
Enhanced Stomatal Conductance Supports Photosynthesis in Wheat to Improved NH4+ Tolerance
Authors: Hu Jinling , Zheng Qiaomei , Dong Chaofeng , Liang Zhihui , Tian Zhongwei , Dai Tingbo
Published: December 2023

The impact of ammonium (NH4+) stress on plant growth varies across species and cultivars, necessitating an in-depth exploration of the underlying response mechanisms. This study delves into elucidating the photosynthetic responses and differences in tolerance to NH4+ stress by investigating the effects on two wheat (Triticum aestivum L.) cultivars, Xumai25 (NH4+-less sensitive) and Yangmai20 (NH4+-sensitive). The cultivars were grown under hydroponic conditions with either sole ammonium nitrogen (NH4+, AN) or nitrate nitrogen (NO3−, NN) as the nitrogen source. NH4+ stress exerted a profound inhibitory effect on seedling growth and photosynthesis in wheat. However, these effects were less pronounced in Xumai25 than in Yangmai20. Dynamic photosynthetic analysis revealed that the suppression in photosynthesis was primarily attributed to stomatal limitation associated with a decrease in leaf water status and osmotic potential. Compared to Yangmai20, Xumai25 exhibited a significantly higher leaf K+ concentration and TaAKT1 upregulation, leading to a stronger stomatal opening and, consequently, a better photosynthetic performance under NH4+ stress. In conclusion, our study suggested stomatal limitation as the primary factor restricting photosynthesis under NH4+ stress. Furthermore, we demonstrated that improved regulation of osmotic substances contributed to higher stomatal conductance and enhanced photosynthetic performance in Xumai25.
Proteomic Analysis Revealed the Antagonistic Effect of Decapitation and Strigolactones on the Tillering Control in Rice
Authors: Zhao Yanhui , Zha Manrong , Xu Congshan , Hou Fangxu , Wang Yan
Published: December 2023

Removing the panicle encourages the growth of buds on the elongated node by getting rid of apical dominance. Strigolactones (SLs) are plant hormones that suppress tillering in rice. The present study employed panicle removal (RP) and external application of synthesized strigolactones (GR) to modulate rice bud growth at node 2. We focused on the full-heading stage to investigate proteomic changes related to bud germination (RP-Co) and suppression (GR-RP). A total of 434 represented differentially abundant proteins (DAPs) were detected, with 272 DAPs explicitly specified in the bud germination process, 106 in the bud suppression process, and 28 in both. DAPs in the germination process were most associated with protein processing in the endoplasmic reticulum and ribosome biogenesis. DAPs were most associated with metabolic pathways and glycolysis/gluconeogenesis in the bud suppression process. Sucrose content and two enzymes of sucrose degradation in buds were also determined. Comparisons of DAPs between the two reversed processes revealed that sucrose metabolism might be a key to modulating rice bud growth. Moreover, sucrose or its metabolites should be a signal downstream of the SLs signal transduction that modulates rice bud outgrowth. Contemplating the result so far, it is possible to open new vistas of research to reveal the interaction between SLs and sucrose signaling in the control of tillering in rice.
Mitigating Salinity Stress in Quinoa (Chenopodium quinoa Willd.) with Biochar and Superabsorber Polymer Amendments
Authors: Derbali Imed , Derbali Walid , Gharred Jihed , Manaa Arafet , Slama Inès , Koyro Hans-Werner
Published: December 2023

In agriculture, soil amendments are applied to improve soil quality by increasing the water retention capacity and regulating the pH and ion exchange. Our study was carried out to investigate the impact of a commercial biochar (Bc) and a superabsorbent polymer (SAP) on the physiological and biochemical processes and the growth performance of Chenopodium quinoa (variety ICBA-5) when exposed to high salinity. Plants were grown for 25 days under controlled greenhouse conditions in pots filled with a soil mixture with or without 3% Bc or 0.2% SAP by volume before the initiation of 27 days of growth in hypersaline conditions, following the addition of 300 mM NaCl. Without the Bc or soil amendments, multiple negative effects of hypersalinity were detected on photosynthetic CO2 assimilation (Anet minus 70%) and on the production of fresh matter from the whole plant, leaves, stems and roots (respectively, 55, 46, 64 and 66%). Moreover, increased generation of reactive oxygen species (ROS) was indicated by higher levels of MDA (plus 142%), antioxidant activities and high proline levels (plus 311%). In the pots treated with 300 mM NaCl, the amendments Bc or SAP improved the plant growth parameters, including fresh matter production (by 10 and 17%), an increased chlorophyll content by 9 and 13% and Anet in plants (by 98 and 115%). Both amendments (Bc and SAP) resulted in significant salinity mitigation effects, decreasing proline and malondialdehyde (MDA) levels whilst increasing both the activity of enzymatic antioxidants and non-enzymatic antioxidants that reduce the levels of ROS. This study confirms how soil amendments can help to improve plant performance and expand the productive range into saline areas.
Utilization of Waste Natural Fibers Mixed with Polylactic Acid (PLA) Bicomponent Fiber: Incorporating Kapok and Cattail Fibers for Nonwoven Medical Textile Applications
Authors: Srisuk Tanyalak , Charoenlarp Khanittha , Kampeerapappun Piyaporn
Published: December 2023

Disposable surgical gowns are usually made from petroleum-based synthetic fibers that do not naturally decompose, impacting the environment. A promising approach to diminish the environmental impact of disposable gowns involves utilizing natural fibers and/or bio-based synthetic fibers. In this study, composite webs from polylactic acid (PLA) bicomponent fiber and natural fibers, cattail and kapok fibers, were prepared using the hot press method. Only the sheath region of the PLA bicomponent fiber melted, acting as an adhesive that enhanced the strength and reduced the thickness of the composite web compared with its state before hot pressing. The mechanical and physical properties of these composite webs were evaluated. Composite webs created from kapok fibers displayed a creamy yellowish-white color, while those made from cattail fibers showed a light yellowish-brown color. Additionally, the addition of natural fibers endowed the composite webs with hydrophobic properties. The maximum natural fiber content, at a ratio of 30:70 (natural fiber to PLA fiber), can be incorporated while maintaining proper water vapor permeability and mechanical properties. This nonwoven material presents an alternative with the potential to replace petroleum-based surgical gowns.