bluuebunny/crossref_metadata_2025 huggingface dataset:
Heterogeneous trajectories of perceived stress and their associations with active leisure: a longitudinal study during the first year of COVID-19
Authors: Kulbin Karel , Jõgi Anna-Liisa , Pulver Aleksander , Kask Kristjan
Published: May 2024

Introduction

There is a plethora of literature on the dynamics of mental health indicators throughout the COVID-19 pandemic, yet research is scarce on the potential heterogeneity in the development of perceived stress. Furthermore, there is a paucity of longitudinal research on whether active leisure engagement, which typically is beneficial in reducing stress, might have similar benefits during times of major disruption. Here we aimed to extend previous work by exploring the dynamics of change in stress and coping, and the associations with active leisure engagement over the first year of COVID-19.

Methods

Data from 439 adults (Mage = 45, SD = 13) in Estonia who participated in a longitudinal online study were analyzed. The participants were assessed at three timepoints: April–May 2020; November–December 2020; and April–May 2021.

Results

Mean stress and coping levels were stable over time. However, latent profile analysis identified four distinct trajectories of change in stress and coping, involving resilient, stressed, recovering, and deteriorating trends. Participants belonging to the positively developing stress trajectories reported higher active leisure engagement than those belonging to the negatively developing stress trajectories.

Discussion

These findings highlight the importance of adopting person-centered approaches to understand the diverse experiences of stress, as well as suggest the promotion of active leisure as a potentially beneficial coping resource, in future crises.
Computer vision syndrome and ergonomic risk factors among workers of the Commercial Bank of Ethiopia in Addis Ababa, Ethiopia: an institutional-based cross-sectional study
Authors: Gasheya Kassahun Ayele , Belay Azanaw Asega , Abegaze Teferi , Zele Yifokire Tefera , Daba Chala
Published: May 2024

Background

Computer vision syndrome (CVS) is the most pressing public health concern that affects vision and reduces quality of life and productivity, particularly in developing countries. Most of the previous studies conducted in Ethiopia focus on the knowledge and personal risk factors of bank workers. Moreover, ergonomic workstation design was not objectively assessed, which could hinder the implementation of effective intervention strategies. Therefore, this study aimed to determine CVS and ergonomic factors among commercial bank workers in Addis Ababa, Ethiopia.

Methods

An institutional-based cross-sectional study was carried out among 466 study participants from May 26 to July 24, 2022. A multistage sampling technique was applied to select the study participants. Data were collected via a standardized tool of CVS (CVS-Q). Besides, workstation ergonomics were pertinently assessed. The collected data was entered into EpiData version 3.1 and exported to SPSS version 26 for data analysis and cleaning. Multivariable logistics regression analysis was performed to identify factors associated with CVS. The variables with a p-value < 0.05 were considered statistically significant factors.

Results

Prevalence of CVS was 75.3% (95% CI: 71.2–79.2%). Blurred vision, eye redness, and headache, 59.8%, 53.7%, and 50.7%, respectively, were frequently reported symptoms. Glare (AOR = 4.45: 95% CI: 2.45–8.08), 20–20–20 principle (AOR = 1.98, 95% CI: 1.06–3.67), wearing non-prescription eyeglasses (AOR = 4.17; 95% CI: 1.92–9.06), and poor workstation (AOR = 7.39; 95% CI: 4.05–13.49) was significantly associated with CVS.

Conclusion

The prevalence of CVS was found to be high. Glare at work, ignoring the 20–20–20 principle, wearing non-prescription eyeglasses, and poor workstation ergonomic design were independent predictors of CVS. Therefore, comprehensive interventional activities like adhering to the 20–20–20 principle, avoiding the use of non-prescription glasses, minimizing glare, and improving workstation ergonomic setup are essential to prevent CVS.
Differences between parents’ and paediatricians’ perceptions of mild respiratory infections in childhood: contrast study
Authors: Ortiz-Gonzalez Luis , Delgado-Ojeda Jesús , Guisado-Rasco Mª Cinta , Santamaria-Orleans Alicia , Coronel-Rodríguez Cristobal
Published: May 2024

Introduction

Mild respiratory infections are a common reason for consultation in paediatrics, both in the emergency department and in primary care clinics. These conditions, mostly viral and self-limiting, have a significant impact on the healthcare system, school and work absenteeism, and family routines. Despite being common and banal illnesses from a medical perspective, they involve a significant concern in families. The main objective of the contrast study was to compare the perceptions of parents and paediatricians regarding mild respiratory infections in childhood and their impact on family conciliation.

Materials and methods

Two online, cross-sectional surveys were conducted among Spanish paediatricians and parents with children aged 6 months to 12 years, involving 504 paediatricians and 1,447 families, with questions on attitudes towards visits to the paediatric consultation, care burden of minor pathologies, work, and family conciliation, and treatment and prevention of these illnesses.

Results

Results showed significant differences in paediatricians’ and parents’ perceptions in many aspects. According to 34.5% of paediatricians and 27% of parents, families regularly go to the paediatrician without a scheduled visit. Only 4% of parents report having self-medicated their child, while paediatricians raise this percentage significantly to 48%. Regarding the question: “it is normal for a child to have an average of 4 colds a year,” only 25.5% of the surveyed families “strongly agree” unlike to 70.2% of paediatricians. 72.8% of paediatricians “strongly agree” with: “in my opinion, it is good for children to get sick to improve their immune system” reduced to 45.9% of parents. Consultations for minor pathologies represent a “high workload” for 60.9% of paediatricians, while this opinion is agreed by only 18.9% of the parents.

Conclusion

Mild respiratory infections in childhood are perceived differently by paediatricians and parents. While paediatricians perceive them as a common and manageable phenomenon, parents tend to show higher concern and demand for medical attention. This study underlines the need to improve communication between paediatricians and parents to align perceptions, optimise the use of the health system resources, and improve the efficiency in the management of these common paediatric illnesses.
Co-creation of a novel approach for improving supply chain management for SARS-CoV-2 point of care diagnostic services in Mopani District, Limpopo Province: nominal group technique
Authors: Maluleke Kuhlula , Musekiwa Alfred , Nxele Siphesihle , Moetlhoa Boitumelo , Makena Langa , Nzuza Nkosingiphile , Lenders Alarice , Manentsa Ncomeka , Maswanganyi Tiyiselani , Dlangalala Thobeka , Mashamba-Thompson Tivani
Published: May 2024

Introduction

Effective supply chain management (SCM) of point-of-care (POC) tests for diseases like severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) requires active participation from diverse stakeholders, government entities, and regulatory bodies. The responsibility for overseeing various aspects of POC tests, including procurement, quality assurance, storage, inventory management, distribution, and human resource capacity, lies with national, provincial, and local levels of government. This study aimed to collaboratively develop an innovative approach to enhance SCM for SARS-CoV-2 POC diagnostic services in resource-limited settings, using the Mopani District in Limpopo province, South Africa, as a case study.

Methods

Key stakeholders were invited to participate in an online workshop using purposive sampling. The study employed the nominal group technique (NGT) for data collection, which consisted of two phases. Phase 1 focused on identifying barriers in the supply chain of COVID-19 rapid tests, while phase 2 aimed to devise strategies to overcome the priority barriers identified in phase 1. Participants used a Likert scale of 1–5 to rank barriers and strategies, and an overall ranking score was calculated for each. The participants were provided with the results of the ranking exercise for their feedback.

Results

Eleven key stakeholders from national (n = 1), provincial (n = 4), and local government (n = 2) levels, research entities (n = 3), and non-governmental organizations (n = 1) took part in the study. Participants identified significant barriers in the supply chain, such as the availability of testing kits, unknown demand, information on SCM during a pandemic, methods of controlling stock, and procurement processes. Strategies suggested by key stakeholders included monitoring stock levels and optimizing stock visibility systems to improve test availability, enhancing information visibility and consistent data updates to address unknown demand and improve SCM during a pandemic, employing data capturing and digitization for effective stock control, and implementing demand planning and standardized procurement processes at the national level to enhance stock procurement.

Discussion

The successful collaboration with key stakeholders, facilitated by the NGT, resulted in the co-creation of a novel approach to enhance SCM for COVID-19 diagnostic services in resource-limited settings. This study holds the potential to support the provision of COVID-19 diagnostic services in such settings. A recommended follow-up study would assess the feasibility of implementing this approach.
Leveraging diffusion models for unsupervised out-of-distribution detection on image manifold
Authors: Liu Zhenzhen , Zhou Jin Peng , Weinberger Kilian Q.
Published: May 2024

Out-of-distribution (OOD) detection is crucial for enhancing the reliability of machine learning models when confronted with data that differ from their training distribution. In the image domain, we hypothesize that images inhabit manifolds defined by latent properties such as color, position, and shape. Leveraging this intuition, we propose a novel approach to OOD detection using a diffusion model to discern images that deviate from the in-domain distribution. Our method involves training a diffusion model using in-domain images. At inference time, we lift an image from its original manifold using a masking process, and then apply a diffusion model to map it towards the in-domain manifold. We measure the distance between the original and mapped images, and identify those with a large distance as OOD. Our experiments encompass comprehensive evaluation across various datasets characterized by differences in color, semantics, and resolution. Our method demonstrates strong and consistent performance in detecting OOD images across the tested datasets, highlighting its effectiveness in handling images with diverse characteristics. Additionally, ablation studies confirm the significant contribution of each component in our framework to the overall performance.
Post-play communication of emotions facilitates prosociality and cooperation
Authors: Gately J. Braxton , Alsharawy Abdelaziz
Published: May 2024

Social decisions with monetary consequences are often accompanied with emotional consequences. Previous studies document a robust role of pre-play message communication in facilitating pro-sociality and cooperation. Yet, the effects of communicating emotional experiences in social interactions (particularly post-play) remain understudied. Here, we examine the value of a social environment where emotional expressions are shared post-play in contrast to a private environment where emotion exposure is absent. We develop an experimental design that facilitates emotion exposure and can be readily administered in or outside the laboratory. In this pre-registered online study, participants (N = 196) completed incentivized extensions of the Dictator Game (DG) and the Prisoners' Dilemma Game (PD). Participants learned to classify their emotional experiences on the arousal, valence, and dominance dimensions using non-verbal pictorial representations. Our experiment comprised both a within-subject and a between-subject manipulation: each participant completed a control condition (C, no emotional exposure) as well as an emotion exposure condition (Emotions), but the type of exposure varied between subjects (certain exposure, or Emotions-E, or probabilistic exposure, or Emotions-P). In all conditions, participants complete a one-shot DG and PD. We find that emotion exposure increases other-regarding behavior under both Emotions-E and Emotion-P conditions in the DG and under Emotions-E only in PD. Further, we find that demand for emotion exposure is hardly driven by the outcome of the social interaction (or the actions selected). We also document how empathic concern influence other-regarding behavior and how reports of emotional experiences vary across treatments and with the different outcomes of social interactions. Our results highlight the integral role of emotion exposure in social decision making. Environments that facilitate the communication of emotional experiences increase pro-sociality and encourage cooperation.
Incidence and predictors of postoperative complications in Sub-Saharan Africa: a systematic review and meta-analysis
Authors: Yadeta Daniel Aboma , Manyazewal Tsegahun , Demessie Dereje Bayissa , Kleive Dyre
Published: May 2024

Background

Postoperative complications remain a significant challenge, especially in settings where healthcare access and infrastructure disparities exacerbate. This systematic review and meta-analysis aimed to determine the pooled incidence and risk factors of postoperative complications among patients undergoing essential surgery in Sub-Saharan Africa (SSA).

Method

PubMed/MEDLINE, EMBASE, CINAHL, Web of Science, and Google Scholar were searched from January 2010 to November 2022 for completed studies reporting the incidence and risk factors associated with postoperative complications among patients undergoing essential surgery in SSA. Severity of postoperative complications was ranked based on the Clavien-Dindo classification system, while risk factors were classified into three groups based on the Donabedian structure-process-outcome quality evaluation framework. Studies quality was appraised using the JBI Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI), and data were analyzed using Comprehensive Meta-Analysis (CMA) software. The study protocol adhered to the PRISMA guidelines and was registered in PROSPERO (CRD42023414342).

Results

The meta-analysis included 19 studies (10 cohort and 9 cross-sectional) comprising a total of 24,136 patients. The pooled incidence of postoperative complications in SSA was 20.2% (95% CI: 18.7%–21.8%), with a substantial heterogeneity of incidence observed. The incidence varied from 14.6% to 27.5% based on the Clavien-Dindo classification. The random-effects model indicated significant heterogeneity among the studies (Q = 54.202, I = 66.791%, p < 0.001). Contributing factors to postoperative complications were: structure-related factors, which included the availability and accessibility of resources, as well as the quality of both the surgical facility and the hospital.; process-related factors, which encompassed surgical skills, adherence to protocols, evidence-based practices, and the quality of postoperative care; and patient outcome-related factors such as age, comorbidities, alcohol use, and overall patient health status.

Conclusion

The meta-analysis reveals a high frequency of postoperative complications in SSA, with noticeable discrepancies among the studies. The analysis highlights a range of factors, encompassing structural, procedural, and patient outcome-related aspects, that contribute to these complications. The findings underscore the necessity for targeted interventions aimed at reducing complications and improving the overall quality of surgical care in the region.

Systematic Reviews Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier (CRD42023414342).
PETRAS: a socio-technical framework for Internet of Things research and development
Authors: Ogunniye Gideon , Hana Amaya , Watson Jeremy
Published: May 2024

This paper presents a case application of a socio-technical framework for Internet of Things (IoT) research and development in the United Kingdom. Applying a socio-technical system approach to IoT, this paper seeks to provide a clear understanding of the interplay between technical and non-technical aspects of IoT research and development. It describes the socio-technical requirements for IoT design and development and provides the current snapshot of research in the United Kingdom to meet these requirements. Finally, the paper provides useful information on how to conceptualize IoT research within human-centered contexts and a useful guide for centre design and evaluation to those developing new research centres or seeking to reinvigorate existing ones.
Assessing the German Act on Corporate Due Diligence Obligations in Supply Chains: a perspective from the smallholder cocoa farmer
Authors: Kraft Sarah Katharina , Quayson Matthew , Kellner Florian
Published: May 2024

This research examines the potential outputs, outcomes, and impacts of the German Act on Corporate Due Diligence Obligations in Supply Chains (LkSG) on the smallholder cocoa farmers in West Africa. The study primarily relies on a literature review and an impact pathway to conduct a systematic analysis to identify the potential effects of the LkSG on smallholder cocoa farmers. The findings indicate that some, but not all of the risks addressed by the LkSG align with those faced by smallholder cocoa farmers and their families. Additionally, the research also reveals weaknesses, particularly in managing environmental risks, which the LkSG does not adequately cover. Our findings show that in the short- and medium-term, the LkSG has no potential effects on smallholder cocoa farmers. Furthermore, the potential positive impacts of the law on smallholder cocoa farmers will take a long time to realize, as the LkSG considers primarily tier-1 suppliers. Companies in Germany might reassess their supply chains to strive for an LkSG-risk-free supply chain, which could in the long term have sustained impacts on smallholder cocoa farmers. However, we recommend a comprehensive risk analysis of the cocoa supply chain to enhance the human rights of cocoa farmers.
Combined associations of regular exercise and work-related moderate-to-vigorous physical activity with occupational stress responses: a cross-sectional study
Authors: Abe Takafumi , Okuyama Kenta , Motohiro Atsushi , Shiratsuchi Daijo , Isomura Minoru
Published: May 2024

Objective

The association between work-related moderate-to-vigorous physical activity (MVPA) and higher levels of stress response is recognized, but whether this association is moderated by regular exercise remains unclear. This cross-sectional study investigated whether exercise-based physical activity (PA) associates with lower levels of stress responses moderated by work-related MVPA.

Methods

The study participants comprised 863 workers from 35 small and medium-sized enterprises in Shimane prefecture, Japan, collected through convenient sampling from April 2021 to August 2022. The Brief Job Stress Questionnaire was used to assess stress responses. Work-related MVPA and exercise-based PA were measured using questionnaires. Multiple linear regression was used to analyze the combined variables of work-related MVPA and exercise-based PA. The reference group had no weekly exercise-based PA and >60 min of work-related MVPA.

Results

When work-related MVPA exceeded 60 min/day, flexibility activity or walking for ≥5 days/week (B = −3.53, 95% CI = −5.96, −1.11; B = −2.53, 95% CI = −4.90, −0.16) and muscle-strengthening activity 1–3 times/week (B = −3.52, 95% CI = −6.91, −0.12) were significantly associated with lower psychological stress response. Flexibility activity (B = −1.74, 95% CI = −3.01, −0.46) showed a similar link with physical stress response. When work-related MVPA was below 60 min/day, flexibility activity (B = −3.23, 95% CI = −6.01, −0.44; B = −3.29, 95% CI = −5.94, −0.63) or walking (B = −4.03, 95% CI = −6.62, −1.45; B = −3.10, 95% CI = −5.76, −0.44) practice 1–4 times/week and ≥5 times/week was significantly associated with lower psychological stress response.

Conclusion

Exercise-based PA greatly and consistently associates with a lower level of stress responses moderated by work-related MVPA.
Landscape connectivity in extensive livestock farming: an adaptive approach to the land sharing and land sparing dilemma
Authors: Pulido-Herrera Luz Astrid , Sepulveda Claudia , Jiménez Jose Antonio , Betanzos Simon Juan Eduardo , Pérez-Sánchez Edwin , Niño Larry
Published: May 2024

This study investigates the “land sharing” versus “land sparing” dilemma in the context of extensive cattle ranching in Chiapas, Mexico. Employing a comprehensive methodology that synthesizes various systems and uses a normalized matrix for relative priority assessment, we identified several geographic variables as zoning criteria. These criteria encompass the hemerobic index, proximity to structurally intact forests, fire frequency, and terrain slope, aiming to identify areas optimal for conservation. Our results highlight properties with high conservation potential and propose two distinct connectivity scenarios, both excluding currently preserved areas. The analysis focuses on the interplay between connectivity and hemeroby, identifying human-influenced regions within the landscape and emphasizing the importance of tree conservation in agricultural contexts for biodiversity preservation. By tackling the “land sharing” vs. “land sparing” debate, the study underscores the necessity of sustainable livestock practices and the critical role of connectivity in ranching landscapes for ecosystem preservation.
Adenocarcinoma developing from gastric heterotopic pancreas: a case report and short review
Authors: Qi Ran , Li Kun , Shi Baomin
Published: May 2024

Heterotopic pancreas is a relatively rare condition that may be associated to clinical complaints or signs. Here, we report a case of gastric heterotopic pancreas assictaed to ductal adenocarcinoma. Obstructive jaundice was the initial symptom prompting medical intervention. A 73-year-old male patient presented with yellow staining of the skin and sclera, and dull epigastric pain. Contrast-enhanced computed tomography showed stenosis of the extrahepatic distal bile duct and mass lesions of the antrum. The patient underwent tumor resection, distal gastrectomy (Billroth II), and common bile duct exploration. Postoperative pathological examination revealed an adenocarcinoma located in the wall of the gastric antrum. Immunohistochemical results suggested that the tumor originated from the pancreas. Heterologous pancreatic tissue and a dilated pancreatic duct were found in the tumor. These findings suggest malignant transformation of the gastric heterotopic pancreas. Of note, jaundice as clinical complaint for adenocarcinoma associated to gastric heterotopic pancreas.
Behavioral consistency of competitive behaviors and feeding patterns in lactating dairy cows across stocking densities at the feed bunk
Authors: Reyes Faith S. , White Heather M. , Weigel Kent A. , Van Os Jennifer M. C.
Published: May 2024

Introduction

High feed bunk stocking densities can differentially impact individual dairy cows’ competitive behaviors, feeding patterns, and feed efficiency. Our objective was to manipulate feed bunk stocking densities to evaluate intra-individual behavioral consistency across stocking densities and quantify associations with feed efficiency and production.

Methods

Thirty-two primiparous (130.7 ± 29.0 days in milk, DIM) and 32 multiparous (111.3 ± 38.3 DIM) lactating Holstein cows were housed with 32 roughage intake control (RIC) bins. Each cow was assigned to share 8 bins with others of the same parity and similar body weight (16 cows/block; 2:1 feed bunk stocking density except during tests). Competition and feeding patterns were evaluated via video and RIC data, respectively, at 3 stocking densities (1:1, 2:1, 4:1 cows/bin) during 1-h tests (2 tests/stocking density; 6 tests/cow) following 2 h feed deprivation. Residual feed intake (RFI) was calculated across the 45-d study as the difference between observed and predicted dry matter intake (DMI) after accounting for known energy sinks. Linear mixed models were used to evaluate the overall impact of test stocking density on competition and feeding patterns. To evaluate intra-individual consistency between stocking densities, individual stability statistic (ISS) scores were computed. Correlational relationships were determined between RFI and ISS scores.

Results and dicsussion

Cows displayed the most competitive behaviors at 2:1 stocking density (p < 0.0001) but experienced the highest rate of contacts per minute of eating time at 4:1 (1:1 vs. 2:1 vs. 4:1: 0.09 vs. 0.95 vs. 1.60 contacts/min; p < 0.0001). Feeding patterns were modulated as stocking density increased; eating rate increased (0.16 vs. 0.18 vs. 0.22 kg/min; p < 0.001) as eating time (40.3 vs. 28.2 vs. 14.6 min; p < 0.001) and DMI decreased (6.3 vs. 5.1 vs. 3.0 kg; p < 0.001). As stocking density doubled, individuals remained consistent (p = 0.018) in time spent near others actively eating and tended to remain consistent in competition behavior and feeding patterns (0.053 ≤ p ≤ 0.094). Between 2:1 and 4:1, cows with higher DMI and milk production were more consistent in first-visit DMI and duration. Feed efficiency was not associated with behavioral consistency across the tests (p ≥ 0.14). Nonetheless, feed bunk stocking density has behavioral implications which should be considered in nutritional management decisions.
Identification of equine mares as reservoir hosts for pathogenic species of Leptospira
Authors: Hamond Camila , Adam Emma N. , Stone Nathan E. , LeCount Karen , Anderson Tammy , Putz Ellie J. , Camp Patrick , Hicks Jessica , Stuber Tod , van der Linden Hans , Bayles Darrell O. , Sahl Jason W. , Schlater Linda K. , Wagner David M. , Nally Jarlath E.
Published: May 2024

Equine leptospirosis can result in abortion, stillbirth, neonatal death, placentitis, and uveitis. Horses can also act as subclinical reservoir hosts of infection, which are characterized as asymptomatic carriers that persistently excrete leptospires and transmit disease. In this study, PCR and culture were used to assess urinary shedding of pathogenic Leptospira from 37 asymptomatic mares. Three asymptomatic mares, designated as H2, H8, and H9, were PCR-positive for lipL32, a gene specific for pathogenic species of Leptospira. One asymptomatic mare, H9, was culture-positive, and the recovered isolate was classified as L. kirschneri serogroup Australis serovar Rushan. DNA capture and enrichment of Leptospira genomic DNA from PCR-positive, culture-negative samples determined that asymptomatic mare H8 was also shedding L. kirschneri serogroup Australis, whereas asymptomatic mare H2 was shedding L. interrogans serogroup Icterohaemorrhagiae. Sera from all asymptomatic mares were tested by the microscopic agglutination test (MAT) and 35 of 37 (94.6%) were seropositive with titers ranging from 1:100 to 1:3200. In contrast to asymptomatic mares, mare H44 presented with acute spontaneous abortion and a serum MAT titer of 1:102,400 to L. interrogans serogroup Pomona serovar Pomona. Comparison of L. kirschneri serogroup Australis strain H9 with that of L. interrogans serogroup Pomona strain H44 in the hamster model of leptospirosis corroborated differences in virulence of strains. Since lipopolysaccharide (LPS) is a protective antigen in bacterin vaccines, the LPS of strain H9 (associated with subclinical carriage) was compared with strain H44 (associated with spontaneous abortion). This revealed different LPS profiles and immunoreactivity with reference antisera. It is essential to know what species and serovars of Leptospira are circulating in equine populations to design efficacious vaccines and diagnostic tests. Our results demonstrate that horses in the US can act as reservoir hosts of leptospirosis and shed diverse pathogenic Leptospira species via urine. This report also details the detection of L. kirschneri serogroup Australis serovar Rushan, a species and serotype of Leptospira, not previously reported in the US.
Outcomes of root canal treatments with three different sealers for 120 fractured maxillary fourth premolar teeth in small-to medium-sized dogs
Authors: Kwon Daehyun , Yoo Dae Sung , Kang Seong Soo , Jang Kwangsik , Kim Se Eun
Published: May 2024

Introduction

Tooth fracture is one of the most common traumatic maxillofacial injuries in dogs and cats. For fractures with pulp exposure occurring in functionally important teeth, the literature indicates that root canal treatment (RCT) is an effective therapy option that may be the remedy of choice before extraction. The most commonly reported fractures in the United States involve canine teeth; however, fractures of the maxillary fourth premolars are more common in Korea, where there are many small-and medium-sized dogs. RCT mechanically and chemically removes pulp tissue and bacteria (cleaning and shaping) from the infected root canal, and obturates the root canal with filling material to restore tooth functionality without inflammation. Various techniques, instruments, and materials used in humans have been modified for application in veterinary dentistry.

Methods

This study analyzed the results of RCT of the maxillary fourth premolar in 120 small-and medium-sized dogs (weighing less than 25 kg) using three different sealers (silicone-based sealer, bioceramic sealer, and calcium hydroxide-based sealer) through a simple application of the single-cone technique.

Results

The overall success rate of RCT in maxillary fourth premolars was 90.83%, with 8.33% no evidence of failure (NEF) and 0.83% failure.

Discussion

There were no significant differences between the three different sealers. Furthermore, preexisting periapical lesion (PAL) was reconfirmed as a factor in reducing the success rate of RCT. In addition, the working length and master apical file of each root were analyzed in our study as a novel reference for endodontic veterinarians.
Metagenomic comparison of gut communities between wild and captive Himalayan griffons
Authors: Wang You , Zhai Jundie , Tang Boyu , Dong Yonggang , Sun Shengzhen , He Shunfu , Zhao Wenxin , Lancuo Zhuoma , Jia Qiangqiang , Wang Wen
Published: May 2024

Introduction

Himalayan griffons (Gyps himalayensis), known as the scavenger of nature, are large scavenging raptors widely distributed on the Qinghai-Tibetan Plateau and play an important role in maintaining the balance of the plateau ecosystem. The gut microbiome is essential for host health, helping to maintain homeostasis, improving digestive efficiency, and promoting the development of the immune system. Changes in environment and diet can affect the composition and function of gut microbiota, ultimately impacting the host health and adaptation. Captive rearing is considered to be a way to protect Himalayan griffons and increase their population size. However, the effects of captivity on the structure and function of the gut microbial communities of Himalayan griffons are poorly understood. Still, availability of sequenced metagenomes and functional information for most griffons gut microbes remains limited.

Methods

In this study, metagenome sequencing was used to analyze the composition and functional structures of the gut microbiota of Himalayan griffons under wild and captive conditions.

Results

Our results showed no significant differences in the alpha diversity between the two groups, but significant differences in beta diversity. Taxonomic classification revealed that the most abundant phyla in the gut of Himalayan griffons were Fusobacteriota, Proteobacteria, Firmicutes_A, Bacteroidota, Firmicutes, Actinobacteriota, and Campylobacterota. At the functional level, a series of Kyoto Encyclopedia of Genes and Genome (KEGG) functional pathways, carbohydrate-active enzymes (CAZymes) categories, virulence factor genes (VFGs), and pathogen-host interactions (PHI) were annotated and compared between the two groups. In addition, we recovered nearly 130 metagenome-assembled genomes (MAGs).

Discussion

In summary, the present study provided a first inventory of the microbial genes and metagenome-assembled genomes related to the Himalayan griffons, marking a crucial first step toward a wider investigation of the scavengers microbiomes with the ultimate goal to contribute to the conservation and management strategies for this near threatened bird.
Rapid Approximation of Low-Thrust Spacecraft Reachable Sets within Complex Two-Body and Cislunar Dynamics
Authors: Bowerfind Sean , Taheri Ehsan
Published: May 2024

The reachable set of controlled dynamical systems is the set of all reachable states from an initial condition over a certain time horizon, subject to operational constraints and exogenous disturbances. In astrodynamics, rapid approximation of reachable sets is invaluable for trajectory planning, collision avoidance, and ensuring safe and optimal performance in complex dynamics. Leveraging the connection between minimum-time trajectories and the boundary of reachable sets, we propose a sampling-based method for rapid and efficient approximation of reachable sets for finite- and low-thrust spacecraft. The proposed method combines a minimum-time multi-stage indirect formulation with the celebrated primer vector theory. Reachable sets are generated under two-body and circular restricted three-body (CR3B) dynamics. For the two-body dynamics, reachable sets are generated for (1) the heliocentric phase of a benchmark Earth-to-Mars problem, (2) two scenarios with uncertainties in the initial position and velocity of the spacecraft at the time of departure from Earth, and (3) a scenario with a bounded single impulse at the time of departure from Earth. For the CR3B dynamics, several cislunar applications are considered, including L1 Halo orbit, L2 Halo orbit, and Lunar Gateway 9:2 NRHO. The results indicate that low-thrust spacecraft reachable sets coincide with invariant manifolds existing in multi-body dynamical environments. The proposed method serves as a valuable tool for qualitatively analyzing the evolution of reachable sets under complex dynamics, which would otherwise be either incoherent with existing grid-based reachability approaches or computationally intractable with a complete Hamilton–Jacobi–Bellman method.
Antimicrobial Activity, Genetic Relatedness, and Safety Assessment of Potential Probiotic Lactic Acid Bacteria Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis) Used as Live Feed in Fish Larviculture
Authors: Contente Diogo , Díaz-Formoso Lara , Feito Javier , Gómez-Sala Beatriz , Costas Damián , Hernández Pablo E. , Muñoz-Atienza Estefanía , Borrero Juan , Poeta Patrícia , Cintas Luis M.
Published: May 2024

Aquaculture is a rapidly expanding agri-food industry that faces substantial economic losses due to infectious disease outbreaks, such as bacterial infections. These outbreaks cause disruptions and high mortalities at various stages of the rearing process, especially in the larval stages. Probiotic bacteria are emerging as promising and sustainable alternative or complementary strategies to vaccination and the use of antibiotics in aquaculture. In this study, potential probiotic candidates for larviculture were isolated from a rotifer-rearing tank used as the first live feed for turbot larvae. Two Lacticaseibacillus paracasei and two Lactiplantibacillus plantarum isolates were selected for further characterization due to their wide and strong antimicrobial activity against several ichthyopathogens, both Gram-positive and Gram-negative. An extensive in vitro safety assessment of these four isolates revealed the absence of harmful traits, such as acquired antimicrobial resistance and other virulence factors (i.e., hemolytic and gelatinase activities, bile salt deconjugation, and mucin degradation, as well as PCR detection of biogenic amine production). Moreover, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) analyses unveiled their genetic relatedness, revealing two divergent clusters within each species. To our knowledge, this work reports for the first time the isolation and characterization of Lactic Acid Bacteria (LAB) with potential use as probiotics in aquaculture from rotifer-rearing tanks, which have the potential to optimize turbot larviculture and to introduce novel microbial management approaches for a sustainable aquaculture.
Examining UV Radiation Patterns in Relation to Particulate Matter and Atmospheric Conditions in Arid, Unclouded Skies
Authors: Maghrabi Abdullrahman , Alharbi Badr , Aldosari Abdulah
Published: May 2024

This study investigates the influences of air temperature (T), relative humidity (RH), wind speed (WS), clearance index (KT), particulate matter (PM) concentrations (PM10 and PM2.5), and aerosol optical depth (AOD) on ultraviolet (UV) radiation during clear skies in Riyadh, central Saudi Arabia. The observational dataset utilized in this study comprises global solar radiation (G), UVA radiation, AOD measurements, and PM concentrations. The data were collected from 2014 to 2015 at the King Abdulaziz City for Science and Technology (KACST) campus in Riyadh. Regression analyses were conducted to investigate the relationships between UV radiation and the considered variables. The methodology is based on the least square method and associated statistical tests. The findings of this study provide valuable insights into the impacts of meteorological variables and aerosols on UV radiation, contributing to the understanding of environmental and industrial applications in the Arabian Peninsula. The analyses showed that the strengths and magnitudes differed from one variable to another. No significant correlations between UVA radiation (315–400 nm) and hourly and daily values of PM2.5 were found. Moreover, no significant correlations were seen between daily values of the UVA radiation and RH and between the UVB (280–315 nm) and PM10. The rest of the correlations (between UV radiation and the PM10 and meteorological variables) were found to be significant. While WS, the ratio of the PM concentrations (PM2.5/PM10), KT, and T exhibited positive correlations with UV radiation, the rest of the variables had anti-correlations with UV radiation. The influences of T, WS, and RH on ambient PM concentrations during the considered period were taken into account, and it was found that the PM concentrations correlate, with different magnitudes and strengths, positively with T and negatively with RH and WS.
Preoperative Molecular Subtype Classification Prediction of Ovarian Cancer Based on Multi-Parametric Magnetic Resonance Imaging Multi-Sequence Feature Fusion Network
Authors: Du Yijiang , Wang Tingting , Qu Linhao , Li Haiming , Guo Qinhao , Wang Haoran , Liu Xinyuan , Wu Xiaohua , Song Zhijian
Published: May 2024

In the study of the deep learning classification of medical images, deep learning models are applied to analyze images, aiming to achieve the goals of assisting diagnosis and preoperative assessment. Currently, most research classifies and predicts normal and cancer cells by inputting single-parameter images into trained models. However, for ovarian cancer (OC), identifying its different subtypes is crucial for predicting disease prognosis. In particular, the need to distinguish high-grade serous carcinoma from clear cell carcinoma preoperatively through non-invasive means has not been fully addressed. This study proposes a deep learning (DL) method based on the fusion of multi-parametric magnetic resonance imaging (mpMRI) data, aimed at improving the accuracy of preoperative ovarian cancer subtype classification. By constructing a new deep learning network architecture that integrates various sequence features, this architecture achieves the high-precision prediction of the typing of high-grade serous carcinoma and clear cell carcinoma, achieving an AUC of 91.62% and an AP of 95.13% in the classification of ovarian cancer subtypes.
Enhancing Osteogenic Potential: Controlled Release of Dopamine D1 Receptor Agonist SKF38393 Compared to Free Administration
Authors: Hua Yunwei , Wang Chenxi , Ge Xiyuan , Lin Ye
Published: May 2024

Osteoporosis is the most common metabolic bone disorder and is characterized by decreased bone density, which has a relationship with the quality of life among the aging population. Previous research has found that activation of the dopamine D1 receptor can improve bone mass formation. SKF38393 is an agonist of dopamine D1 receptors. However, as a small-molecule drug, SKF38393 is unstable and releases quickly. The aim of this study was to prototype polylactic-co-glycolic acid (PLGA)/SKF38393 microspheres and assess their potential osteogenic effects compared to those under the free administration of SKF38393. The cytocompatibility of PLGA/SKF38393 was determined via CCK-8 and live/dead cell staining; the osteogenic effects in vitro were determined with ALP and alizarin red staining, qRT-PCR, and Western blotting; and the in vivo effects were assessed using 25 Balb/c mice. We also used a PCR array to explore the possible signaling pathway changes after employing PLGA/SKF38393. Our experiments demonstrated that the osteogenic effect of D1Rs activated by the PLGA/SKF38393 microsphere was better than that under free administration, both in vitro and in vivo. According to the PCR array, this result might be associated with six signaling pathways (graphical abstract). Ultimately, in this study, we prototyped PLGA/SKF38393, demonstrated its effectiveness, and preliminarily analyzed its mechanism of action.
Michelangelo Effect in Cognitive Rehabilitation: Using Art in a Digital Visuospatial Memory Task
Authors: Salera Claudia , Capua Chiara , De Angelis Domenico , Coiro Paola , Venturiero Vincenzo , Savo Anna , Marinozzi Franco , Bini Fabiano , Paolucci Stefano , Antonucci Gabriella , Iosa Marco
Published: May 2024

The Michelangelo effect is a phenomenon that shows a reduction in perceived effort and an improvement in performance among both healthy subjects and patients when completing a motor task related to artistic stimuli, compared to performing the same task with non-artistic stimuli. It could contribute to the efficacy of art therapy in neurorehabilitation. In this study, the possible occurrence of this effect was tested in a cognitive task by asking 15 healthy subjects and 17 patients with a history of stroke to solve a digital version of the classical memory card game. Three different types of images were used in a randomized order: French cards, artistic portraits, and photos of famous people (to compensate for the possible effects of face recognition). Healthy subjects were involved to test the usability and the load demand of the developed system, reporting no statistically significant differences among the three sessions (p > 0.05). Conversely, patients had a better performance in terms of time (p = 0.014) and the number of attempts (p = 0.007) needed to complete the task in the presence of artistic stimuli, accompanied by a reduction in the perceived effort (p = 0.033). Furthermore, artistic stimuli, with respect to the other two types of images, seemed more associated with visuospatial control than linguistic functions.
Biological Functions and Potential Therapeutic Significance of O-GlcNAcylation in Hepatic Cellular Stress and Liver Diseases
Authors: Mao Zun , Mu Junpeng , Gao Zhixiang , Huang Shile , Chen Long
Published: May 2024

O-linked-β-D-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation), which is dynamically regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), is a post-translational modification involved in multiple cellular processes. O-GlcNAcylation of proteins can regulate their biological functions via crosstalk with other post-translational modifications, such as phosphorylation, ubiquitination, acetylation, and methylation. Liver diseases are a major cause of death worldwide; yet, key pathological features of the disease, such as inflammation, fibrosis, steatosis, and tumorigenesis, are not fully understood. The dysregulation of O-GlcNAcylation has been shown to be involved in some severe hepatic cellular stress, viral hepatitis, liver fibrosis, nonalcoholic fatty acid liver disease (NAFLD), malignant progression, and drug resistance of hepatocellular carcinoma (HCC) through multiple molecular signaling pathways. Here, we summarize the emerging link between O-GlcNAcylation and hepatic pathological processes and provide information about the development of therapeutic strategies for liver diseases.
Evaluation of the Therapeutical Effect of Matricaria Chamomilla Extract vs. Galantamine on Animal Model Memory and Behavior Using 18F-FDG PET/MRI
Authors: Iacob Roxana , Palimariciuc Matei , Florea Tudor , Pricope Cosmin Vasilica , Uritu Cristina Mariana , Tamba Bogdan Ionel , Ionescu Teodor Marian , Stolniceanu Cati Raluca , Jalloul Wael , Dobrin Romeo Petru , Hritcu Lucian , Cioanca Oana , Hancianu Monica , Naum Alexandru Gratian , Stefanescu Cipriana
Published: May 2024

The memory-enhancing activity of Matricaria chamomilla hydroalcoholic extract (MCE) is already being investigated by behavioral and biochemical assays in scopolamine-induced amnesia rat models, while the effects of scopolamine (Sco) on cerebral glucose metabolism are examined as well. Nevertheless, the study of the metabolic profile determined by an enriched MCE has not been performed before. The present experiments compared metabolic quantification in characteristic cerebral regions and behavioral characteristics for normal, only diseased, diseased, and MCE- vs. Galantamine (Gal)-treated Wistar rats. A memory deficit was induced by four weeks of daily intraperitoneal Sco injection. Starting on the eighth day, the treatment was intraperitoneally administered 30 min after Sco injection for a period of three weeks. The memory assessment comprised three maze tests. Glucose metabolism was quantified after the 18F-FDG PET examination. The right amygdala, piriform, and entorhinal cortex showed the highest differential radiopharmaceutical uptake of the 50 regions analyzed. Rats treated with MCE show metabolic similarity with normal rats, while the Gal-treated group shows features closer to the diseased group. Behavioral assessments evidenced a less anxious status and a better locomotor activity manifested by the MCE-treated group compared to the Gal-treated group. These findings prove evident metabolic ameliorative qualities of MCE over Gal classic treatment, suggesting that the extract could be a potent neuropharmacological agent against amnesia.
Effects of Beam Mode on Hole Properties in Laser Processing
Authors: Zhang Tingzhong , Li Hui , Zhang Chengguang , Zhang Aili
Published: May 2024

The laser beam mode affects the power density distribution on the irradiated target, directly influencing the product quality in laser processing, especially the hole quality in laser drilling. The Gaussian beam shape, Mexican-Hat beam shape, Double-Hump beam shape, and Top-Hat beam shape are four typical laser beam modes used as a laser heat source and introduced into our proficient laser-drilling model, which involves complex physical phenomena such as heat and mass transfer, solid/liquid/gas phase changes, and two-phase flow. Simulations were conducted on an aluminum target, and the accuracy was verified using experimental data. The results of the simulations for the fundamental understanding of this laser–material interaction are presented in this paper; in particular, the hole shape, including the depth–diameter ratio and the angle of the cone, as well as spatter phenomena and, thus, the formed recast layer, are compared and analyzed in detail in this paper. This study can provide a reference for the optimization of the laser-drilling process, especially the selection of laser beam mode.