bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in Clean Fuel Ethanol Production from Electro-, Photo- and Photoelectro-Catalytic CO2 Reduction
Authors: Song Yanfang , Chen Wei , Wei Wei , Sun Yuhan
Published: November 2020

Using renewable energy to convert CO2 to a clean fuel ethanol can not only reduce carbon emission through the utilization of CO2 as feedstock, but also store renewable energy as the widely used chemical and high-energy-density fuel, being considered as a perfect strategy to address current environment and energy issues. Developing efficient electrocatalysts, photocatalysts, and photoelectrocatalysts for CO2 reduction is the most crucial keystone for achieving this goal. Considerable progresses in CO2-based ethanol production have been made over the past decades. This review provides the general principles and summarizes the latest advancements in electrocatalytic, photocatalytic and photoelectrocatalytic CO2 conversion to ethanol. Furthermore, the main challenges and proposed future prospects are illustrated for further developments in clean fuel ethanol production.
Immobilized Cell Physiology Imaging and Stabilization of Enzyme Cascade Reaction Using Recombinant Cells Escherichia coli Entrapped in Polyelectrolyte Complex Beads by Jet Break-Up Encapsulator
Authors: Bučko Marek , Gemeiner Peter , Krajčovič Tomáš , Hakarová Marietta , Chorvát Dušan , Chorvátová Alžbeta Marček , Lacík Igor , Rudroff Florian , Mihovilovič Marko D.
Published: November 2020

A novel, high performance, and scalable immobilization protocol using a laminar jet break-up technique was developed for the production of polyelectrolyte complex beads with entrapped viable Escherichia coli cells expressing an enzyme cascade of alcohol dehydrogenase, enoate reductase, and cyclohexanone monooxygenase. A significant improvement of operational stability was achieved by cell immobilization, which was manifested as an almost two-fold higher summative product yield of 63% after five cascade reaction cycles as compared to the yield using free cells of 36% after the maximum achievable number of three cycles. Correspondingly, increased metabolic activity was observed by multimodal optical imaging in entrapped cells, which was in contrast to a complete suppression of cell metabolism in free cells after five reaction cycles. Additionally, a high density of cells entrapped in beads had a negligible effect on bead permeability for low molecular weight substrates and products of cascade reaction.
Optical Transmittance for Strongly-Wedge-Shaped Semiconductor Films: Appearance of Envelope-Crossover Points in Amorphous As-Based Chalcogenide Materials
Authors: Ruiz-Pérez Juan José , Navarro Emilio Márquez
Published: November 2020

In this work, we study the influence of the geometry of a thin film on its transmission spectrum, as measured on amorphous As-based chalcogenide layers grown onto 1-mm-thick soda-lime-silica glass substrates. A new method is suggested for a comprehensive optical characterization of the film-on-substrate specimen, which is based upon some novel formulae for the normal-incidence transmittance of such a specimen. It has to be emphasized that they are not limited to the usual cases, where the refractive index, n, of the film and that of the thick transparent substrate, s, must obey: n2>>k2 and s2>>k2, respectively, where k stands for the extinction coefficient of the semiconductor. New expressions for the top and bottom envelopes of the transmission spectrum are also obtained. The geometry limitation usually found when characterizing strongly-wedge-shaped films, has been eliminated with the introduction of an appropriate parameter into the corresponding equations. The presence of crossover points in the top and bottom envelopes of the transmission spectrum, for these strongly-wedge-shaped chalcogenide samples, has been both theoretically predicted and experimentally confirmed.
Microparticle Deposition on Human Serum Albumin Layers: Unraveling Anomalous Adsorption Mechanism
Authors: Nattich-Rak Małgorzata , Dąbkowska Maria , Adamczyk Zbigniew
Published: November 2020

Human serum albumin (HSA) layers are adsorbed on mica under controlled diffusion transport at pH 3.5 and various ionic strengths. The surface concentration of HSA is directly determined by AFM imaging of single molecules. It is shown that the adsorption kinetics derived in this way is quantitatively described using the random sequential (RSA) adsorption model. The electrokinetic characteristics of the HSA layers at various pHs comprising their zeta potential are acquired in situ while using the streaming potential method. It is shown that at pH 3.5 the zeta potential of mica becomes positive for HSA concentrations above 3000 μm−2. At larger pHs, HSA layers exhibit negative zeta potential for the entire range of coverage. Thorough characteristics of these monolayers at various pHs were performed applying the colloid deposition method involving negatively charged polystyrene microparticles. The kinetics of their deposition and their maximum coverage are determined as a function of the HSA layer surface concentration, pH, and ionic strength. An anomalous deposition of microparticles on substrates also exhibiting a negative zeta potential is observed, which contradicts the Derjaguin, Landau, Vervey, Overbeek (DLVO) theory. This effect is interpreted in terms of heterogeneous charge distribution that results from molecule concentration fluctuations. It is also shown that the maximum concentration of microparticles abruptly decreases with the electric double-layer thickness that is regulated by changing ionic strength, which indicates that their deposition is governed by electrostatic interactions. One can argue that the results obtained in this work can be exploited as useful reference data for the analysis of deposition phenomena of bioparticles on protein layers.
Identification of Local Structure in 2-D and 3-D Atomic Systems through Crystallographic Analysis
Authors: Ramos Pablo , Herranz Miguel , Foteinopoulou Katerina , Karayiannis Nikos , Laso Manuel
Published: November 2020

In the present work, we revise and extend the Characteristic Crystallographic Element (CCE) norm, an algorithm used to simultaneously detect radial and orientational similarity of computer-generated structures with respect to specific reference crystals and local symmetries. Based on the identification of point group symmetry elements, the CCE descriptor is able to gauge local structure with high precision and finely distinguish between competing morphologies. As test cases we use computer-generated monomeric and polymer systems of spherical particles interacting with the hard-sphere and square-well attractive potentials. We demonstrate that the CCE norm is able to detect and differentiate, between others, among: hexagonal close packed (HCP), face centered cubic (FCC), hexagonal (HEX) and body centered cubic (BCC) crystals as well as non-crystallographic fivefold (FIV) local symmetry in bulk 3-D systems; triangular (TRI), square (SQU) and honeycomb (HON) crystals, as well as pentagonal (PEN) local symmetry in thin films of one-layer thickness (2-D systems). The descriptor is general and can be applied to identify the symmetry elements of any point group for arbitrary atomic or particulate system in two or three dimensions, in the bulk or under confinement.
Sympérasmology: A Proposal for the Theory of Synthetic System Knowledge
Authors: Horváth Imre
Published: November 2020

Owing to the results of research in system science, artificial intelligence, and cognitive engineering, engineered systems are becoming more and more powered by knowledge. Complementing common-sense and scientific knowledge, system knowledge is maturing into a crucial productive asset. However, an overall theory of the knowledge of intellectualized systems does not exist. Some researchers suggest that time has come to establish a philosophically underpinned theoretical framework. This motion is seconded by the on-going intelligence revolution, in which artificial intelligence becomes a productive power, enabler of smart systems, and a strong transformer of the social life. The goal of this paper is to propose a skeleton of the needed generic theory of system knowledge (and a possible new branch of philosophical studies). The major assumption is that a significant part of the synthetic system knowledge (SSK) is “sympérasma”, that is, knowledge conjectured, inferred, constructed, or otherwise derived during the operation of systems. This part will become even more dominant in the future. Starting out from the above term, the paper suggests calling this theory “sympérasmology”. Some specific domains of “sympérasmological” studies, such as (i) manifestations of SSK, (ii) mechanisms of generating SSK, (iii) dependability of SSK, (iv) operational power of SSK, (v) composability of SSK, and (vi) advancement of SSK, are identified. It is understood that the essence and status of SSK cannot be investigated without considering the related cognitive processes and technological enablers. The paper presents a number of open questions relevant for follow-up discussions.
“Am I Doing Enough?” Special Educators’ Experiences with Emergency Remote Teaching in Spring 2020
Authors: Schuck Rachel K. , Lambert Rachel
Published: November 2020

While the COVID-19 pandemic radically changed all aspects of everyone’s life, the closure of schools was one of the most impactful, significantly altering daily life for school personnel, students, and families. The shift to Emergency Remote Teaching (ERT) presented particular challenges to special educators of students with significant support needs who often benefit from strong interpersonal connections, modeling, and the use of physical manipulatives. This paper details the experiences of two elementary special education teachers as they navigated the transition to ERT. The teachers reported three distinct stages of ERT: making contact, establishing routines, and transitioning to academics. They also discussed the challenges they faced during this period, such as the inequity in resources amongst their students, needing to rely on at-home support in order to meaningfully teach students, and changes in what it meant to be a teacher while having to teach online. While clearly not in favor of online learning, the teachers do present glimmers of hope, for example, with regards to increased communication between teachers and parents. The challenges and strategies used to overcome these challenges will be of use to educators in the coming months, with implications for distance learning in this population.
Electric Migration of Hydrogen Ion in Pore-Voltammetry Suppressed by Nafion Film
Authors: Liu Ling , Aoki Koichi Jeremiah , Chen Jingyuan
Published: November 2020

Micro-hole voltammetry exhibiting rectified current-voltage curves was performed in hydrochloric acid by varying the lengths and the diameters of the micro-holes on one end of which a Nafion film was mounted. Some voltammetric properties were compared with those in NaCl solution. The voltammograms were composed of two line-segments, the slope of one segment being larger than the other. They were controlled by electric migration partly because of the linearity of the voltammograms and partly the independence of the scan rates. Since the low conductance which appeared in the current from the hole to the Nafion film was proportional to the cross section area of the hole and the inverse of the length of the hole, it should be controlled by the geometry of the hole. The conductance of the hydrogen ion in the Nafion film was observed to be smaller than that in the bulk, because the transport rate of hydrogen ion by the Grotthuss mechanism was hindered by the destruction of hydrogen bonds in the film. In contrast, the conductance for the current from the Nafion to the hole, enhancing by up to 30 times in magnitude from the opposite current, was controlled by the cell geometry rather than the hole geometry except for very small holes. A reason for the enhancement is a supply of hydrogen ions from the Nafion to increase the concentration in the hole. The concentration of the hydrogen ion was five times smaller than that of sodium ion because of the blocking of transport of the hydrogen ion in the Nafion film. However, the rectification ratio of H+ was twice as large as that of Na+.
Modelling Neural Dynamics with Optics: A New Approach to Simulate Spiking Neurons through an Asynchronous Laser
Authors: Rostro-Gonzalez Horacio , Lauterio-Cruz Jesus Pablo , Pottiez Olivier
Published: November 2020

In this paper, we propose a novel approach for implementing spiking neurons through an optical system. Spiking neurons are a new approach to emulate the neural processes that occur in the brain, known as the third generation of artificial neural networks. They have been successfully used to build a new technology called neuromorphic engineering, which looks for a better performance than traditional computing in tasks usually performed by AI-based systems. Our optical system consists of a low-cost laser source, based on a microcontroller and a continuous-wave laser diode; the microcontroller allows producing synchronous or asynchronous pulses with complex time profiles. Here, through said system we have successfully reproduced most of the neural dynamics observed in biological neurons. These dynamics have been reproduced using a very simple optical array with a great potential for the development of neuromorphic systems. The optical system has been experimentally validated.
A 12-bit 40-MS/s SAR ADC with Calibration-Less Switched Capacitive Reference Driver
Authors: Ju Hyungyu , Lee Sewon , Lee Minjae
Published: November 2020

This paper presents a switched capacitive reference driver (SCRD) with a low-energy switching scheme. In order to reduce the performance degradation resulting from a signal-dependent voltage drop in a capacitive reference driver (CRD) without increasing the capacitance (CREF) of a CRD, the proposed SCRD utilizes the CRD for LSB conversion cycles. In MSB conversion cycles, a supply voltage is used as a reference voltage to save on area and power consumption. As such, the proposed SCRD significantly relaxes the required CREF, and does not necessitate bit weight calibration or compensation requiring an auxiliary capacitor-based digital-to-analog converter (CDAC). To evaluate the proposed SCRD, a prototype 12-bit 40-MS/s SAR ADC is fabricated in a 65 nm CMOS process. With near Nyquist frequency, the measured spurious-free dynamic range (SFDR) of the SAR ADC with the SCRD is 80.6 dB, which is about a 16 dB improvement from the SFDR of a SAR ADC with a CRD only.
A Compact 16 Channel Embedded System with High Dynamic Range Readout and Heater Management for Semiconducting Metal Oxide Gas Sensors
Authors: Hammer Christof , Warmer Johannes , Maurer Stephan , Kaul Peter , Thoelen Ronald , Jung Norbert
Published: November 2020

The simultaneous operation of multiple different semiconducting metal oxide (MOX) gas sensors is demanding for the readout circuitry. The challenge results from the strongly varying signal intensities of the various sensor types to the target gas. While some sensors change their resistance only slightly, other types can react with a resistive change over a range of several decades. Therefore, a suitable readout circuit has to be able to capture all these resistive variations, requiring it to have a very large dynamic range. This work presents a compact embedded system that provides a full, high range input interface (readout and heater management) for MOX sensor operation. The system is modular and consists of a central mainboard that holds up to eight sensor-modules, each capable of supporting up to two MOX sensors, therefore supporting a total maximum of 16 different sensors. Its wide input range is archived using the resistance-to-time measurement method. The system is solely built with commercial off-the-shelf components and tested over a range spanning from 100 Ω to 5 GΩ (9.7 decades) with an average measurement error of 0.27% and a maximum error of 2.11%. The heater management uses a well-tested power-circuit and supports multiple modes of operation, hence enabling the system to be used in highly automated measurement applications. The experimental part of this work presents the results of an exemplary screening of 16 sensors, which was performed to evaluate the system’s performance.
Evolutionary Optimization of Asymmetrical Pixelated Antennas Employing Shifted Cross Shaped Elements for UHF RFID
Authors: Mair Dominik , Renzler Michael , Pfeifhofer Alexander , Ußmüller Thomas
Published: November 2020

The design and optimization of antennas for specific boundary conditions and parameters, such as size and frequency, for a given application, is a highly complex and time consuming process, which usually involves elaborate computer-aided software packages and/or methods. Additionally, trade-offs and co-dependencies have to be considered, when optimizing for a specific parameter, i.e., a high antenna gain goes usually hand-in-hand with a large antenna. Therefore, we implemented a method that involves the automated design and optimization of asymmetrical pixelated antennas using evolutionary algorithms, where arbitrary parameters can be optimized for chosen boundary conditions. In contrast to other approaches, shifted cross elements were employed as pixels to avoid point contact defects. We present results for antennas with an exemplary resonant frequency of 868 MHz and sizes of 3 × 3, 4 × 4 and 6 × 6 cm. The agreement between measurements and simulations for the antenna gain and reflection coefficient is excellent, with a maximum error of 1.15% for the single resonant frequency (relative error) and 1.35 dB for the antenna gain (mean absolute error).
Masks, Gloves, and the COVID-19 Pandemic: Rapid Assessment of Public Behaviors in the United States
Authors: Khubchandani Jagdish , Saiki Diana , Kandiah Jayanthi
Published: November 2020

The COVID-19 outbreak was declared a national emergency in the U.S. in March 2020, and in April 2020, the U.S. government authorities issued recommendations on the use of masks and gloves as protective measures. Despite such recommendations, popular media reports highlighted a lack of compliance. However, no systematic study has examined the use of protective strategies (e.g., wearing a mask) by the American public to prevent the spread of COVID-19 during early stages of the pandemic. The purpose of this study was to conduct a rapid national assessment of public behaviors to prevent COVID-19 spread during the early stages of the pandemic and to assess how these behaviors may have differed based on selected sociodemographic characteristics. A total of 835 adult Americans nationwide took a multi-item survey and were asked about wearing masks, gloves, and their demographic background. The majority of the study participants reported wearing a mask more often during the pandemic (76%), but the majority did not wear gloves more often during the pandemic (30%). Significant differences (p < 0.05) for wearing masks were found based on sex, age, ethnicity, marital status, living arrangements, and employment status. For gloves, significant differences were found based on sex, age, marital status, and employment. While the pandemic continues to unfold and with recent reports of a surge in cases in the U.S., public health practitioners and policymakers must emphasize COVID-19 prevention strategies for the general public and explore pragmatic options to increase compliance of protective behaviors among the general public.
Anthropogenic Fires in West African Landscapes: A Spatially Explicit Model Perspective of Humanized Savannas
Authors: Caillault Sébastien , Laris Paul , Fleurant Cyril , Delahaye Daniel , Ballouche Aziz
Published: November 2020

Fire regimes are important components of environmental dynamics, but our understanding of them is limited. Despite recent advances in the methodologies used to remotely sense and map fires and burned areas and new case studies that shed light on local fire use and management practices, the scientific community still has much to learn about anthropogenic fire regimes. We identify two areas for improvement: first, the fine-scale heterogeneity of fire dynamics for specific regions is often masked by global-scale approaches, and second, barriers between the disciplines focusing on fire impacts hamper the development of knowledge of the human dimensions of fire regimes. To address the “blind spot” that these limitations create, we present a simple dynamic model of fire ignition in savanna systems. The aim is to connect the local and global scales of fire regimes by focusing on human fire management (anthropogenic fire). Our dynamical model is based on a study area in Western Burkina Faso and integrates biophysical elements (climate and soil data), land cover, and fire management scenarios based on field surveys. The simulation results offer contrasting views of the impact of local fire management practices on regional fire regimes observed in savannas. Fire density and frequency are local variables that clearly change the fire regimes despite a complex and constrained biophysical system. This experience, drawing from fieldwork and modelling, may be a way to integrate some key aspects of anthropogenic fire research in savanna systems.
Morphology, Transcriptomics and In Vitro Model of Skin from Polar Cod (Boreogadus Saida) and Atlantic Cod (Gadus Morhua)
Authors: Ytteborg Elisabeth , Hansen Øyvind Johannes , Høst Vibeke , Afanasyev Sergey , Vieweg Ireen , Nahrgang Jasmine , Krasnov Aleksei
Published: November 2020

Fish skin is a multifunctional barrier tissue with high regeneration capacity that interacts with the surrounding environment and provides protection. Functional importance, high complexity and activity make skin an attractive tissue for studying the effects of environmental challenges and chemical stressors in fish. The aim of this work was to characterize skin from polar cod (Boreogadus saida) and Atlantic cod (Gadus morhua), and to test cod skin as an in vitro model in exposure studies. Both species have similar skin structures including epidermis, mucous cells, club cells and scales. However, microarchitectural differences were detected; Atlantic cod has a smooth epidermal surface and overlapping scales, whereas polar cod has a folded outer surface and discontinuous scales. Genome-wide microarray found 6.5k genes with expression differences, which suggested more active turnover of proteins, proliferation and motility of cells in skin of polar cod. Hydrogen peroxide (H2O2) was used to examine skin responses. Transcriptome response was stronger in the skin of polar cod, with 155 differentially expressed genes. The skin from Atlantic cod was further used to develop a cell culture. H2O2 decreased the cell migration rate in a dose-dependent manner, which could indicate reduced skin healing capacity. The results revealed novel skin structures and confirmed that the skin from cod is a promising tissue for evaluation of stressors.
A Near-Shore Linear Wave Model with the Mixed Finite Volume and Finite Difference Unstructured Mesh Method
Authors: Lai Yong G. , Kim Han Sang
Published: November 2020

The near-shore and estuary environment is characterized by complex natural processes. A prominent feature is the wind-generated waves, which transfer energy and lead to various phenomena not observed where the hydrodynamics is dictated only by currents. Over the past several decades, numerical models have been developed to predict the wave and current state and their interactions. Most models, however, have relied on the two-model approach in which the wave model is developed independently of the current model and the two are coupled together through a separate steering module. In this study, a new wave model is developed and embedded in an existing two-dimensional (2D) depth-integrated current model, SRH-2D. The work leads to a new wave–current model based on the one-model approach. The physical processes of the new wave model are based on the latest third-generation formulation in which the spectral wave action balance equation is solved so that the spectrum shape is not pre-imposed and the non-linear effects are not parameterized. New contributions of the present study lie primarily in the numerical method adopted, which include: (a) a new operator-splitting method that allows an implicit solution of the wave action equation in the geographical space; (b) mixed finite volume and finite difference method; (c) unstructured polygonal mesh in the geographical space; and (d) a single mesh for both the wave and current models that paves the way for the use of the one-model approach. An advantage of the present model is that the propagation of waves from deep water to shallow water in near-shore and the interaction between waves and river inflows may be carried out seamlessly. Tedious interpolations and the so-called multi-model steering operation adopted by many existing models are avoided. As a result, the underlying interpolation errors and information loss due to matching between two meshes are avoided, leading to an increased computational efficiency and accuracy. The new wave model is developed and verified using a number of cases. The verified near-shore wave processes include wave shoaling, refraction, wave breaking and diffraction. The predicted model results compare well with the analytical solution or measured data for all cases.
A Review of Registered Clinical Trials on Dietary (Poly)Phenols: Past Efforts and Possible Future Directions
Authors: Marino Mirko , Del Bo’ Cristian , Martini Daniela , Porrini Marisa , Riso Patrizia
Published: November 2020

In recent years, the increasing number of studies on polyphenol demonstrates the efforts in elucidating the potential role of these bioactives on human health. This study reviews the main topics and characteristics of clinical trials on polyphenols registered over the last 20 years, in order to track past and current efforts as well as to highlight the main research gaps in this field. The review was conducted by collecting trials registered in ClinicalTrials.gov and International Standard Randomised Controlled Trial Number (ISRCTN) registry. Overall, 750 clinical trials were selected and included in the final evaluation. Most of the trials were performed on extracts or pure compounds followed by studies conducted on polyphenol-rich foods, in particular berries. A total of 520 clinical trials focused on health effects, 55 on bioavailability, and 175 on both. Regarding outcomes, 139 registered intervention studies had the lipid profile and blood pressure as primary outcomes. The overview provided by this analysis also emphasizes the emerging interest in new outcomes related to polyphenols intervention such as microbiota composition and the evaluation of inter-individual variability in response to the intake of polyphenols. Our review underlines the need of further trials covering unexplored or debated research aspects and provides insights for the design and development of future intervention studies and related research areas.
All You Need Is Light. Photorepair of UV-Induced Pyrimidine Dimers
Authors: Banaś Agnieszka Katarzyna , Zgłobicki Piotr , Kowalska Ewa , Bażant Aneta , Dziga Dariusz , Strzałka Wojciech
Published: November 2020

Although solar light is indispensable for the functioning of plants, this environmental factor may also cause damage to living cells. Apart from the visible range, including wavelengths used in photosynthesis, the ultraviolet (UV) light present in solar irradiation reaches the Earth’s surface. The high energy of UV causes damage to many cellular components, with DNA as one of the targets. Putting together the puzzle-like elements responsible for the repair of UV-induced DNA damage is of special importance in understanding how plants ensure the stability of their genomes between generations. In this review, we have presented the information on DNA damage produced under UV with a special focus on the pyrimidine dimers formed between the neighboring pyrimidines in a DNA strand. These dimers are highly mutagenic and cytotoxic, thus their repair is essential for the maintenance of suitable genetic information. In prokaryotic and eukaryotic cells, with the exception of placental mammals, this is achieved by means of highly efficient photorepair, dependent on blue/UVA light, which is performed by specialized enzymes known as photolyases. Photolyase properties, as well as their structure, specificity and action mechanism, have been briefly discussed in this paper. Additionally, the main gaps in our knowledge on the functioning of light repair in plant organelles, its regulation and its interaction between different DNA repair systems in plants have been highlighted.
Exploring the Impact of Obesity on Health Care Resources and Coding in the Acute Hospital Setting: A Feasibility Study
Authors: Tan Winnie S. Y. , Young Adrienne M. , Di Bella Alexandra L. , Comans Tracy , Banks Merrilyn
Published: November 2020

Obesity is costly, yet there have been few attempts to estimate the actual costs of providing hospital care to the obese inpatient. This study aimed to test the feasibility of measuring obesity-related health care costs and accuracy of coding data for acute inpatients. A prospective observational study was conducted over three weeks in June 2018 in a single orthopaedic ward of a metropolitan tertiary hospital in Queensland, Australia. Demographic data, anthropometric measurements, clinical characteristics, cost of hospital encounter and coding data were collected. Complete demographic, anthropometric and clinical data were collected for all 18 participants. Hospital costing reports and coding data were not available within the study timeframe. Participant recruitment and data collection were resource-intensive, with mobility assistance required to obtain anthropometric measurements in more than half of the participants. Greater staff time and costs were seen in participants with obesity compared to those without obesity (obesity: body mass index ≥ 30), though large standard deviations indicate wide variance. Data collected suggest that obesity-related cost and resource use amongst acute inpatients require further exploration. This study provides recommendations for protocol refinement to improve the accuracy of data collected for future studies measuring the actual cost of providing hospital care to obese inpatients.
Molecular Characterization of Terpenoid Biosynthetic Genes and Terpenoid Accumulation in Phlomis umbrosa Turczaninow
Authors: Hwang Si-Yoon , Lee Sang-Hoon , Lee Young-Seob , Han Sin-Hee , Song Beom-Heon , Reddy Chinreddy Subramanyam , Kim Yeon Bok , Park Sang Un
Published: November 2020

The root of Phlomis umbrosa has traditionally been used as a medicine in South Asian nations to treat colds and bone fractures, to staunch bleeding, and as an anti-inflammatory, and such use continues today. We identified 10 genes that are involved in terpenoid biosynthesis, while using the Illumina/Solexa HiSeq2000 platform. We investigated the transcript levels of the 10 genes using quantitative real-time PCR and quantified the level of terpenoid accumulation in different organs of P. umbrosa while using high-performance liquid chromatography. The transcript levels of PuHDR and PuHMGR1 were the highest among the studied genes. Sesamoside, an iridoid glycoside, appeared in higher quantity than shanzhiside methylester, umbroside (8-O-acetyl shanzhiside methyl ester), and acteoside. We speculate that PuHDR and PuHMGR1 may contribute to terpenoid biosynthesis in P. umbrosa. This study highlights the molecular mechanisms that underlie iridoid glycoside biosynthesis in P. umbrosa.
Discovery of the Environmental Factors Affecting Urban Dwellers’ Mental Health: A Data-Driven Approach
Authors: Wu Chao , Zheng Pei , Xu Xinyuan , Chen Shuhan , Wang Nasi , Hu Simon
Published: November 2020

Mental health is the foundation of health and happiness as well as the basis for an individual’s meaningful life. The environmental and social health of a city can measure the mental state of people living in a certain areas, and exploring urban dwellers’ mental states is an important factor in understanding and better managing cities. New dynamic and granular urban data provide us with a way to determine the environmental factors that affect the mental states of urban dwellers. The characteristics of the maximal information coefficient can identify the linear and nonlinear relationships so that we can fully identify the physical and social environmental factors that affect urban dwellers’ mental states and further test these relationships through linear and nonlinear modeling. Taking the Greater London as an example, we used data from the London Datastore to discover the environmental factors that had the highest correlation with urban mental health from 2015 to 2017 and to prove that they had a high nonlinear correlation through neural network modeling. This paper aimed to use a data-driven approach to find environmental factors that had not yet received enough attention and to provide a starting point for research by establishing hypotheses for further exploration of the impact of environmental factors on mental health.
Connections between Family Assets and Positive Youth Development: The Association between Parental Monitoring and Affection with Leisure-Time Activities and Substance Use
Authors: Belintxon Maider , Osorio Alfonso , de Irala Jokin , Van Riper Marcia , Reparaz Charo , Vidaurreta Marta
Published: November 2020

This study aimed to determine the associations between parental monitoring and affection and three adolescent lifestyle aspects: constructive leisure, non-constructive leisure and substance use. A cross-sectional study was conducted in four countries (Chile, Mexico, Spain and Peru). Adolescents aged 12–15 self-completed a multi-purpose questionnaire. Multiple logistic regressions were performed to analyse the association between the parental monitoring and affection variables and the outcomes in terms of the children’s lifestyles. The results indicate that parental monitoring is conducive to more constructive leisure and less non-constructive leisure and seems to be conducive to the prevention of substance use. Furthermore, parental affection is conducive to constructive leisure and the prevention of substance use. The discussion focuses on the fact that the family can be a protective resource associated with positive adolescent development.
Effect of Water-Saving Society Policy on Water Consumption in the Cities of China: A Propensity Score Matching Analysis
Authors: Zhao Yali , Li Min
Published: November 2020

The increased demand for water resources due to urban population and economic growth has worsened the urban water crisis. In order to address this issue, a policy of “developing a water-saving society” (namely, water-saving society policy) has been implemented in some Chinese cities. This study takes 285 cities at the prefecture level and above as the sample and uses the propensity score matching (PSM) method to analyze the effect of China’s urban water-saving society policy on the reduction of water consumption per CNY 10,000 gross domestic product (GDP) from 2005 to 2017. The results show that the water-saving society policy significantly (p < 0.01) reduced water consumption in the study period; however, the effects differed between cities with different water resource endowments, economic development level, and urban scale. Specifically, there was a positive water consumption reduction effect in cities in humid areas, with low economic development, or of large scale, while the effect was limited in cities in arid areas, with high economic development, or of small scale. Therefore, for areas where water resource supply is insufficient, water-saving policy should be designed and implemented suiting local conditions, and it is also necessary to explore more water sources.
A 2-Month Follow-Up Study of Psychological Distress among Italian People during the COVID-19 Lockdown
Authors: Roma Paolo , Monaro Merylin , Colasanti Marco , Ricci Eleonora , Biondi Silvia , Di Domenico Alberto , Verrocchio Maria Cristina , Napoli Christian , Ferracuti Stefano , Mazza Cristina
Published: November 2020

The spread of coronavirus disease 2019 (COVID-19) has called for unprecedented measures, including a national lockdown in Italy. The present study aimed at identifying psychological changes (e.g., changes in depression, stress, and anxiety levels) among the Italian public during the lockdown period, in addition to factors associated with these changes. An online follow-up survey was administered to 439 participants (original sample = 2766), between 28 April and 3 May 2020. A paired sample t-test tested for differences in stress, anxiety, and depression over the period. Multivariate regression models examined associations between sociodemographic variables, personality traits, coping strategies, depression, and stress. Results showed an increase in stress and depression over the lockdown, but not anxiety. Negative affect and detachment were associated with higher levels of depression and stress. Higher levels of depression at the start of the lockdown, as well as fewer coping strategies and childlessness, were associated with increased depression at follow-up, whereas higher levels of stress at the start of the lockdown and younger age were associated with higher stress at follow-up. These results may help us to identify persons at greater risk of suffering from psychological distress as a result lockdown conditions, and inform psychological interventions targeting post-traumatic symptoms.
How Sexuality Education Programs Have Been Evaluated in Low- and Lower-Middle-Income Countries? A Systematic Review
Authors: Ivanova Olena , Rai Masna , Michielsen Kristien , Dias Sónia
Published: November 2020

Background: Complex sexual and reproductive health interventions, such as sexuality education (SE), contain multiple components and activities, which often requires a comprehensive evaluation design and adaptation to a specific context. In this review, we synthetize available scientific literature on types of evaluation designs used for SE programs in low- and lower-middle-income countries. Methods: Two databases yielded 455 publications, from which 20 articles met the inclusion criteria. Narrative synthesis was used to summarize the findings. Evaluation approaches were compared to recommended evaluation frameworks. The quality of articles was assessed by using MMAT 2018. Results: A total of 15 interventions employed in 10 countries were evaluated in the 20 selected articles, with the quality of publications being moderate to high. Randomized controlled trial was the predominant study design, followed by quasi-experimental design. There were seven process evaluation studies, using mixed methods. Main outcomes reported were of public health or behavioral nature—condom use, sexual debut or delay, and number of sexual partners. By comparing evaluation designs to recommended frameworks, few studies fulfilled at least half of the criteria. Conclusions: Evaluations of SE are largely dominated by quantitative (quasi-)experimental designs and use of public health outcomes. To improve understanding of SE program effectiveness, it is important to assess the quality of the program development, its implementation, and its impact, using existing evaluation frameworks and recommendations.
Alternatives to Insulin for the Regulation of Blood Sugar Levels in Type 2 Diabetes
Authors: Bondy Stephen C. , Wu Meixia , Prasad Kedar N.
Published: November 2020

This short overview focuses on the causation and treatment of type 2 diabetes (T2D). Emphasis is given to the historical basis of understanding this disease and the background leading to emergence of the central role of insulin. The strengths of insulin administration in the treatment of diabetes are profound, but these need to be balanced against several serious shortcomings of its extended use. Some alternative approaches to T2D management are considered. Insulin is no longer considered as the first choice for type 2 diabetes, and an expanding range of new therapeutic possibilities is emerging. While these may lack the potency of insulin, at a minimum, they allow a major reduction in the intensity of insulin use. In view of the rising worldwide incidence of this disease, it is imperative to develop safe and inexpensive means of limiting its potential for impairment of normal functioning.
The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning
Authors: Rekstina Valentina V. , Sabirzyanova Tatyana A. , Sabirzyanov Fanis A. , Adzhubei Alexei A. , Tkachev Yaroslav V. , Kudryashova Irina B. , Snalina Natalia E. , Bykova Anastasia A. , Alessenko Alice V. , Ziganshin Rustam H. , Kuznetsov Sergei A. , Kalebina Tatyana S.
Published: November 2020

Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW). Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension is poorly understood. We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment. Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins. Correlation of Bgl2 distribution among pools and its N-glycosylation was not found. Glutathione affects Bgl2 conformation, probably resulting in the mode of its attachment and enzymatic activity. Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW. Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization. The hypothetical scheme of distribution of Bgl2 inside CW is represented.
The Role of Chrysoperla carnea (Steph.) (Neuroptera: Chrysopidae) as a Potential Dispersive Agent of Noctuid Baculoviruses
Authors: Gutiérrez-Cárdenas Oscar , Adán Ángeles , Beperet Inés , Medina Pilar , Caballero Primitivo , Garzón Agustín
Published: November 2020

Baculoviruses (BV) are highly effective against lepidopteran pests of economic importance such as Spodoptera exigua. The combined use of entomopathogens and macrobiological control agents requires the study of their relationships. Laboratory bioassays were developed to evaluate the interactions between the multiple nucleopolyhedroviruses of S. exigua (SeMNPV) and Autographa californica (AcMNPV), and the predator Chrysoperla carnea. The microscopic examination of predator’s excreta (larval drops and meconia) after the ingestion of BV-infected S. exigua revealed the presence of viral occlusion bodies (OBs). The reinfection of S. exigua larvae with BVs-contaminated excreta by using OBs water suspensions or by direct application both yielded high mortality values but different speed-of-kill results. Meconia killed before in suspensions due to their higher viral load and larval excretion drops did so in direct application due to their liquid nature and their easiness of consumption. The prey-mediated ingestion of SeMNPV and AcMNPV triggered slight effects in C. carnea, which were probably derived from the food nutritional quality. Chrysoperla carnea larvae did not discriminate between healthy and BV-infected S. exigua, while a preference was shown for S. exigua (healthy or infected) vs. Macrosiphum euphorbiae. Our findings present C. carnea, and particularly its larvae, as a promissory candidate for BV dispersion in the field.
Genetic Diversity of Mitochondrial DNA of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Associated with Cassava and the Occurrence of Cassava Mosaic Disease in Zambia
Authors: Chiza Chikoti Patrick , Tembo Mathias , Peter Legg James , Rufini Shirima Rudolph , Mugerwa Habibu , Sseruwagi Peter
Published: November 2020

Bemisia tabaci is an important vector of cassava brown streak viruses and cassava mosaic begomoviruses, the causal agents of cassava brown streak disease and cassava mosaic disease (CMD), respectively. A study was carried out to determine the genetic variability of B. tabaci associated with cassava and the occurrence of CMD in Zambia in 2013 and 2015. Phylogenetic analysis showed the presence of only the sub-Saharan Africa 1 (SSA1) genetic group in Zambia. The SSA1 population had three population subgroups (SGs): SSA1-SG1, SSA1-SG2 and SSA1-SG3. All three SSA1 population subgroups occurred in Western Province. However, only SSA1-SG3 occurred in Eastern Province, while only SSA1-SG1 occurred in North Western and Luapula Provinces. Adult B. tabaci were most abundant in Western Province in 2013 (11.1/plant) and 2015 (10.8/plant), and least abundant (0.2/plant) in Northern Province in both 2013 and 2015. CMD was prevalent in all seven provinces surveyed, with the highest incidence recorded in Lusaka Province in both 2013 (78%) and 2015 (83.6%), and the lowest in Northern Province in both 2013 (26.6%) and 2015 (29.3%). Although SSA1-SG1 occurred at greater abundances than the other subgroups, there was no direct association demonstrated between whitefly subgroup and incidence of CMD. Establishing which B. tabaci genetic groups and populations are associated with CMD and their distribution in the country is a key factor in guiding the development of CMD control strategies for cassava-dependent households.
Comparative Morphology of the Mouthparts in Three Predatory Stink Bugs (Heteroptera: Asopinae) Reveals Feeding Specialization of Stylets and Sensilla
Authors: Wang Yan , Brożek Jolanta , Dai Wu
Published: November 2020

Mouthpart structures were observed in three species of Asopinae using scanning electron microscopy to investigate their morphological disparity. The examined species attack mainly slow-moving, soft-bodied insects, primarily larval forms of the Lepidoptera, and are the natural enemies of many pests. This is the first detailed description of their external mouthparts. The triangular and elongated labrum and four-segmented tube-like labium are longer in Picromerus species (Picromerus bidens (Linnaeus, 1758) and Picromerus lewisi Scott, 1874 than in Cazira bhoutanica Schouteden, 1907. The labrum of P. lewisi and C. bhoutanica appear to be equipped with olfactory sensilla basiconica Sb3, a special type of sensilla with nanopores. The labium surface in all studied species bears 14 types of sensilla (St1–St4, Sb1–7, Sst, Sca1–2). A new characteristic of sensilla trichodea is represented in sensillum St1; in both Picromerus species, it is classified as an olfactory sensillum with nanopores. The tripartite apex of the labium consists of two lateral lobes and a central membranous lobe having microtrichial extensions. Each lobe has one sensory field, including sensilla basiconica (Sb7), sensilla styloconica (Sst), and sensilla trichodea (St4). In the three studied predatory stink bugs, each mandibular stylet tip has five irregular teeth and three long, pointed hooks. The two opposing maxillae, which are held together by a tongue-and-groove system, form a food canal and a salivary canal. The apices of the right maxilla have small teeth and few short barbs along the edge of the food canal. In P. bidens and P. lewisi, there are 5 teeth, while in C. bhoutanica there are 2. Based on structural differences, we inferred that the hook-shaped mandibular teeth, right maxilla with small teeth, and few short barbs along edge of the food canal are more adapted for a predatory lifestyle. Predatory stink bugs use sharp recurved hooks and irregular teeth penetrating, tearing, or filing devices that aid in the mechanical disruption of host tissue. Stiff bristles in the food canal may indicate their possible adaptation to feeding on insect larvae. The evolution of mouthpart morphology and the putative functional significance of sensilla are discussed, providing insight into the sensory mechanism.