bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between furosemide administration and outcomes in critically ill patients with acute kidney injury
Authors: Zhao Guang-ju , Xu Chang , Ying Jian-chao , Lü Wen-biao , Hong Guang-liang , Li Meng-fang , Wu Bing , Yao Yong-ming , Lu Zhong-qiu
Published: March 2020

Background

Although current guidelines for AKI suggested against the use of furosemide in AKI management, the effect of furosemide on outcomes in real-world clinical settings remains uncertain. The aim of the present study was to investigate the association between furosemide administration and outcomes in critically ill patients with AKI using real-world data.

Methods

Critically ill patients with AKI were identified from the Medical Information Mart for Intensive Care (MIMIC)-III database. Propensity score (PS) matched analysis was used to match patients receiving furosemide to those without diuretics treatment. Linear regression, logistic regression model, and Cox proportional hazards model were used to assess the associations between furosemide and length of stay, recovery of renal function, and in-hospital and 90-day mortality, respectively.

Results

A total of 14,154 AKI patients were included in the data analysis. After PS matching, 4427 pairs of patients were matched between the patients who received furosemide and those without diuretics treatment. Furosemide was associated with reduced in-hospital mortality [hazard ratio (HR) 0.67; 95% CI 0.61–0.74; P < 0.001] and 90-day mortality [HR 0.69; 95% CI 0.64–0.75; P < 0.001], and it was also associated with the recovery of renal function [HR 1.44; 95% CI 1.31–1.57; P < 0.001] in over-all AKI patients. Nevertheless, results illustrated that furosemide was not associated with reduced in-hospital mortality in patients with AKI stage 0–1 defined by UO criteria, AKI stage 2–3 according to SCr criteria, and in those with acute-on-chronic (A-on-C) renal injury.

Conclusions

Furosemide administration was associated with improved short-term survival and recovery of renal function in critically ill patients with AKI. Furosemide was especially effective in patients with AKI UO stage 2–3 degree. However, it was not effective in those with AKI SCr stage 2–3 and chronic kidney disease. The results need to be verified in randomized controlled trials.
A nurse-led health coaching intervention for stroke survivors and their family caregivers in hospital to home transition care in Chongqing, China: a study protocol for a randomized controlled trial
Authors: Lin Shuanglan , Xiao Lily Dongxia , Chamberlain Diane
Published: March 2020

Background

Hospital to home transition care is a most stressful period for stroke survivors and their caregivers to learn self-management of stroke-related health conditions and to engage in rehabilitation. Health coaching has been identified as a strategy to enhance self-management of poststroke care at home. However, interventions in this field that are informed by a health coaching framework are scarce. This study will address a gap in research by testing the hypothesis that a nurse-led health coaching intervention can improve health outcomes for stroke survivors and their family caregivers in hospital to home transition care.

Methods

This is a single-blind, two-arm parallel randomized controlled trial of a nurse-led health coaching program versus routine care situated in two tertiary hospitals in Chongqing, China. Stroke survivors and their primary family caregivers will be recruited together as “participant dyads”, and the estimated sample size is 140 (70 in each group). The intervention includes a 12-week nurse-led health coaching program in hospital to home transition care commencing at discharge from the hospital. The primary outcome is changes in self-efficacy of stroke survivors at 12 weeks from the baseline. The secondary outcomes are changes in stroke survivors’ and quality of life, functional ability, stroke-related knowledge, the number of adverse events, and unplanned hospital admissions, and caregivers’ self-efficacy and caregiver-related burden at 12 weeks from the baseline. The outcomes will be measured at 12 weeks and 24 weeks from the baseline.

Discussion

This study will examine the effect of nurse-led health coaching on hospital to home transition care for stroke survivors and their caregivers. It is anticipated that findings from this trial will provide research evidence to inform policy, and resource and practice development to improve hospital to home transition care for stroke survivors and their caregivers.

Trial registration

The Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN12619000321145. Registered on 1 March 2019.
Linker histone variant H1t is closely associated with repressed repeat-element chromatin domains in pachytene spermatocytes
Authors: Mahadevan Iyer Aditya , Kumar Sanjeev , Rao Manchanahalli R. Satyanarayana
Published: March 2020

Background

H1t is the major linker histone variant in pachytene spermatocytes, where it constitutes 50–60% of total H1. This linker histone variant was previously reported to localize in the nucleolar rDNA element in mouse spermatocytes. Our main aim was to determine the extra-nucleolar localization of this linker histone variant in pachytene spermatocytes.

Results

We generated H1t-specific antibodies in rabbits and validated its specificity by multiple assays like ELISA, western blot, etc. Genome-wide occupancy studies, as determined by ChIP-sequencing in P20 mouse testicular cells revealed that H1t did not closely associate with active gene promoters and open chromatin regions. Annotation of H1t-bound genomic regions revealed that H1t is depleted from DSB hotspots and TSS, but are predominantly associated with retrotransposable repeat elements like LINE and LTR in pachytene spermatocytes. These chromatin domains are repressed based on co-association of H1t observed with methylated CpGs and repressive histone marks like H3K9me3 and H4K20me3 in vivo. Mass spectrometric analysis of proteins associated with H1t-containing oligonucleosomes identified piRNA–PIWI pathway proteins, repeat repression-associated proteins and heterochromatin proteins confirming the association with repressed repeat-element genomic regions. We validated the interaction of key proteins with H1t-containing oligonucleosomes by use of ChIP-western blot assays. On the other hand, we observe majority of H1t peaks to be associated with the intergenic spacer of the rDNA element, also in association with SINE elements of the rDNA element. Thus, we have identified the genomic and chromatin features of both nucleolar and extranucleolar localization patterns of linker histone H1t in the context of pachytene spermatocytes.

Conclusions

H1t-containing repeat-element LINE and LTR chromatin domains are associated with repressive marks like methylated CpGs, histone modifications H3K9me3 and H4K20me3, and heterochromatin proteins like HP1β, Trim28, PIWIL1, etc. Apart from localization of H1t at the rDNA element, we demonstrate the extranucleolar association of this linker histone variant at repeat-associated chromatin domains in pachytene spermatocytes. We hypothesize that H1t might induce local chromatin relaxation to recruit heterochromatin and repeat repression-associated protein factors necessary for TE (transposable element) repression, the final biological effect being formation of closed chromatin repressed structures.
Insensitivity versus poor response to tumour necrosis factor inhibitors in rheumatoid arthritis: a retrospective cohort study
Authors: Ochi Sae , Saito Kazuyoshi , Mizoguchi Fumitaka , Kato Shigeaki , Tanaka Yoshiya
Published: March 2020

Background

With advancement in the treatment options of rheumatoid arthritis (RA), optimising the outcomes of difficult-to-treat patients has become increasingly important in clinical practice. In particular, insensitivity to first-line biologic disease-modifying anti-rheumatic drugs (bDMARD) is becoming a significant problem because it may decrease the treatment adherence of patients. This study aimed to compare RA patients with an insensitivity and those with a poor response to initial treatment with tumour necrosis factor inhibitors (TNFis), which are the most frequently used bDMARDs.

Methods

This is a retrospective cohort study using clinical data from the FIRST registry. bDMARD-naïve RA patients treated with tumour necrosis factor inhibitors (TNFis) from August 2003 to May 2019 were included and categorised into three groups: TNFi insensitivity, poor response to TNFis and controls. TNFi insensitivity was defined as follows: (1) discontinuation of TNFi treatment within 22 weeks due to lack of any response, or (2) an increase in the disease activity score in 28 joints–C-reactive protein (DAS28-CRP) of > 0.6 at week 22 compared with week 0. Among the remaining patients, those with a DAS28-CRP > 2.6 at week 22 were categorised in the poor response group.

Results

Of the included patients, 94 were classified in the insensitivity, 604 in the poor response and 915 in the control. A higher DAS28-CRP before treatment was a risk factor for a poor response but not for insensitivity. In contrast, dose escalation of infliximab decreased the risk of a poor response but not that of insensitivity.

Conclusions

In future research, poor and insensitivity to bDMARDs should be assessed separately to fully elucidate the aetiology of, and risk factors for, bDMARD refractoriness.
Factors related to poor adherence in Latvian asthma patients
Authors: Smits Dins , Brigis Girts , Pavare Jana , Urtane Inga , Kovalovs Sandis , Barengo Noël Christopher
Published: March 2020

Background

The problem of nonadherence to therapy is a key reason of insufficient asthma control. Evaluating the beliefs about asthma medication, cognitive and emotional perceptions may help to identify patients with poor adherence to treatment in clinical practice which need additional attention in order to increase the likelihood of them taking their asthma medication according to the prescribed treatment protocol. The purpose of this study is to assess whether beliefs about asthma medication, cognitive and emotional factors are related to poor treatment adherence of asthma medication in a sample of asthma patients in Latvia.

Methods

Study subjects were asthma patients attending outpatient pulmonologist consultations in Latvia during September 2013 to December 2015. Beliefs about asthma medicine, cognitive and emotional factors related to asthma were determined in a cross-sectional, self-administered survey. The validated Beliefs about Medicines Questionnaire (BMQ) and the Brief Illness Perception Questionnaire (brief IPQ) were used. Treatment adherence was assessed using 5-item version of the Medication Adherence Reporting Scale (MARS). The total sample size was 352 patients. Logistic regression models were used to predict poor adherence to asthma treatment. The validity of each logistic regression model was assessed by the Hosmer/Lemeshow test. The main outcome measure was self-reported adherence to treatment.

Results

The more the patients agreed with the statement “My future health depends on my asthma medication” the lower the possibility of poor adherence to asthma treatment (OR 0.42; 95% CI 0.24–0.74). The more concerned the patients were in regard to long-term effects of their medication (OR 2; 95% CI 1.22–3.27), the higher the probability of poor treatment adherence.

Conclusions

Screening asthma patients using the BMQ may help to identify those to benefit from interventions targeting their concerns and medication beliefs in order to improve adherence to asthma medication.
Mesenchymal stem cell-derived exosomes protect beta cells against hypoxia-induced apoptosis via miR-21 by alleviating ER stress and inhibiting p38 MAPK phosphorylation
Authors: Chen Jin , Chen Junqiu , Cheng Yuanhang , Fu Yunfeng , Zhao Hongzhou , Tang Minying , Zhao Hu , Lin Na , Shi Xiaohua , Lei Yan , Wang Shuiliang , Huang Lianghu , Wu Weizhen , Tan Jianming
Published: March 2020

Background

Hypoxia is a major cause of beta cell death and dysfunction after transplantation. The aim of this study was to investigate the effect of exosomes derived from mesenchymal stem cells (MSCs) on beta cells under hypoxic conditions and the potential underlying mechanisms.

Methods

Exosomes were isolated from the conditioned medium of human umbilical cord MSCs and identified by WB, NTA, and transmission electron microscopy. Beta cells (βTC-6) were cultured in serum-free medium in the presence or absence of exosomes under 2% oxygen conditions. Cell viability and apoptosis were analysed with a CCK-8 assay and a flow cytometry-based annexin V-FITC/PI apoptosis detection kit, respectively. Endoplasmic reticulum stress (ER stress) proteins and apoptosis-related proteins were detected by the WB method. MiRNAs contained in MSC exosomes were determined by Illumina HiSeq, and treatment with specific miRNA mimics or inhibitors of the most abundant miRNAs was used to reveal the underlying mechanism of exosomes.

Results

Exosomes derived from MSC-conditioned culture medium were 40–100 nm in diameter and expressed the exosome markers CD9, CD63, CD81, HSP70, and Flotillin 1, as well as the MSC markers CD73, CD90, and CD105. Hypoxia significantly induced beta cell apoptosis, while MSC exosomes remarkably improved beta cell survival. The WB results showed that ER stress-related proteins, including GRP78, GRP94, p-eIF2α and CHOP, and the apoptosis-related proteins cleaved caspase 3 and PARP, were upregulated under hypoxic conditions but were inhibited by MSC exosomes. Moreover, the p38 MAPK signalling pathway was activated by hypoxia and was inhibited by MSC exosomes. The Illumina HiSeq results show that MSC exosomes were rich in miR-21, let-7 g, miR-1246, miR-381, and miR-100. After transfection with miRNA mimics, the viability of beta cells under hypoxia was increased significantly by miR-21 mimic, and the p38 MAPK and ER stress-related proteins in beta cells were downregulated. These changes were reversed after exosomes were pretreated with miR-21 inhibitor.

Conclusions

Exosomes derived from MSCs could protect beta cells against apoptosis induced by hypoxia, largely by carrying miR-21, alleviating ER stress and inhibiting p38 MAPK signalling. This result indicated that MSC exosomes might improve encapsulated islet survival and benefit diabetes patients.
Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells
Authors: Fang Fang , Li Zili , Zhao Qian , Xiong Chengliang , Ni Ke
Published: March 2020

Background

In mammals, specification of primordial germ cells (PGCs) is established in the early post­implantation embryo. The bone morphogenetic protein (BMP)-SMAD and WNT3-β-catenin signaling initiate the gene regulatory network for PGC specification. The activation of SOX17-BLIMP1 axis is critical for human PGC program. Moreover, EpCAM and INTEGRINα6 were identified as surface markers of human PGC-like cells (PGCLCs) recently. However, the signaling mechanism for PGC specification in non­rodent mammals remains to be clarified.

Methods

We differentiated human induced pluripotent stem cells (hiPSCs) into PGCLCs in vitro in response to Activin A and BMP4. The percentage of EpCAM/INTEGRINα6 double-positive cells (PGCLCs) was analyzed by flow cytometry. The expression of PGC genes was evaluated by qRT-PCR and immunofluorescence. The expression dynamic of multi-lineage genes during the differentiation process was evaluated by qRT-PCR.

Results

Under the stimulation for PGCLC induction, the embryoids derived from hiPSCs initiated significant upregulation of the early PGC genes (BLIMP1, TFAP2C, and NANOS3), but maintained low or no levels of DPPA3 and late PGC genes (DAZL and DDX4). The percentage of EpCAM/INTEGRINα6 double-positive PGCLCs reached the highest at day 6 of induction. After pre-induction, the incipient mesoderm-like cells (iMeLCs) upregulated most of the mesoderm genes (EOMES, T, MSXI, RUNX2, and MIXL1). The differentiating embryoids showed high levels of key pluripotency genes, OCT4 and NANOG, but became negative for SOX2. In contrast to iMeLCs, the differentiating embryoids downregulated mesoderm genes RUNX2 and EOMES, and ectoderm gene PAX6, but increased the expression of endoderm gene SOX17.

Conclusions

During PGCLC induction process in vitro, the differentiating embryoids not only activated the PGC-related genes, but also displayed complex regulation of pluripotency genes and multi-lineage genes. These results would be meaningful for future research investigating the regulation of human early germ line development.
Standardized GMP-compliant scalable production of human pancreas organoids
Authors: Dossena Marta , Piras Roberta , Cherubini Alessandro , Barilani Mario , Dugnani Erica , Salanitro Francesca , Moreth Till , Pampaloni Francesco , Piemonti Lorenzo , Lazzari Lorenza
Published: March 2020

Background

Organoids are three-dimensional in vitro-grown cell clusters that recapitulate key features of native organs. In regenerative medicine, organoid technology represents a promising approach for the replacement of severely damaged organs, such as the pancreas in patients with type 1 diabetes. Isolation human pancreas organoids (hPOs) in chemically defined serum-free culture media would be a major milestone for this approach.

Methods

Starting from discarded pancreatic tissues, we developed a large-scale process for obtaining clinically relevant quantities of undifferentiated organoids, obviating enzymatic digestion and operator-dependent pancreatic ducts picking steps. hPO identity was characterized by molecular and flow cytometry analysis.

Results

This work demonstrates that it is possible to obtain a large-scale production of organoids. We introduced some innovations in the isolation, expansion, and freezing of hPOs from five donors. First of all, the choice of the starting material (islet-depleted pancreas) that allows obtaining a high quantity of hPOs at low passages. On the other hand, we introduced mechanical dissociation and we eliminated the picking step to exclude the operator-depending steps, without affecting the success of the culture (100% success rate). Another important improvement was to replace R-spondin-1 (Rspo1) conditioned medium with Rspo1 recombinant molecule to obtain a well-defined composition of the expansion medium. Finally, we implemented a GMP-compliant freezing protocol. hPOs showed exponential growth with diameter and area that increased three- and eight-fold in 7 days, respectively. Immunophenotypic profile and gene expression analysis revealed that hPOs were composed of ductal (82.33 ± 8.37%), acinar (2.80 ± 1.25%) cells, and pancreatic progenitors (5.81 ± 2.65%).

Conclusion

This work represents a milestone for a GMP-compliance hPO production and, ultimately, their clinical application as a type 1 diabetes therapy.
The challenge of developing human 3D organoids into medicines
Authors: Vives Joaquim , Batlle-Morera Laura
Published: March 2020

The capacity of organoids to generate complex 3D structures resembling organs is revolutionizing the fields of developmental and stem cell biology. We are currently establishing the foundations for translational applications of organoids such as drug screening, personalized medicine and launching the future of cell therapy using organoids. However, clinical translation of organoids into cell replacement therapies is halted due to (A) a few preclinical studies demonstrating their efficacy and (B) the lack of robust, reproducible, and scalable methods of production in compliance with current pharmaceutical standards. In this issue of Stem Cell Research & Therapy [ref], Dossena and collaborators present a validated bioprocess design for large-scale production of human pancreatic organoids from cadaveric tissue in accordance with current good manufacturing practice. The authors also propose a set of specifications of starting materials and critical quality attributes of final products that are of interest to other developments provided that this type of medicines are different than any other medicinal product due to their complex composition and living nature of the active ingredient. Although large-scale production of functional cells secreting insulin is still a challenge, the development of methods such as the one presented by Dossena and collaborators contributes to move toward clinical use of organoids in the treatment of type 1 diabetes and opens avenues for future clinical use of organoids in degenerative pathologies.
Joint beamforming and power allocation for wireless powered UAV-assisted cooperative NOMA systems
Authors: Mu Guangchen
Published: March 2020

In this paper, we investigate an unmanned aerial vehicle (UAV)-enabled non-orthogonal multiple access (NOMA) systems, where UAV acts as a full-duplex (FD) relay to help the communication between the base station (BS) and two NOMA users. Assume that the UAV follows a circular trajectory and applies decode-and-forward (DF) strategy. Using simultaneous wireless information and power transfer (SWIPT), the UAV harvests energy from the BS in the first time slot and self-interference due to FD mode in the second time slot. By the joint optimization of beamforming and time allocation ratio, we aim at maximizing sum throughput of the whole system and harvested energy at UAV. To solve two highly non-convex problem, we propose the corresponding algorithms based on inner approximation method, respectively, which can converge to at least optimal solutions in few steps. In terms of two different system performances, numerical results can verify that the effectiveness of the proposed scheme. We also find the optimal azimuth angle of UAV’s circular trajectory by simulation.
Health-risk behavior differences between boarding and non-resident students: Brazilian adolescent National School Health Survey
Authors: Noll Matias , Noll Priscilla Rayanne e Silva , Tiggemann Carlos Leandro , Custodio Daniela Costa , Silveira Erika Aparecida
Published: March 2020

Background

Studies that evaluated health-risk behaviors with boarding students are scarce. There are no studies with representative samples among adolescents residing in educational institutions in Latin America. To better assess the role of resident status on such behaviors, this study aimed to compare health-risk behaviors between boarding and non-resident students assessed by the Brazilian National Adolescent School Health Survey (PeNSE).

Methods

A cross-sectional study was conducted using the 2015 PeNSE database. A sample of 101,788 students (aged 11–19 years) from both public and private schools throughout all the Brazilian states completed the survey. A self-administered questionnaire was used to evaluate multiple health-related behaviors (sociodemographic characteristics; sexual behavior; cigarette use; drug use; and alcohol use). Poisson regression model-based analyses were performed and the effects measured through the prevalence ratio (PR) and its 95% confidence interval (CI).

Results

Boarding residents reported more health-risk behaviors than non-residents: previous sexual intercourse (PR 1.17, 1.10–1.25), smoking experience (PR 1.12, 1.03–1.21), monthly smoking frequency (PR 1.68, 1.42–1.99), monthly alcohol intake (PR 2.12, 1.79–2.50), inebriation (PR 1.51, 1.35–1.71), drug use experience (PR 1.23, 1.10–1.38), and monthly drug use frequency (PR 1.59, 1.31–1.94).

Conclusions

Boarding residents reported more health-risk behaviors than did non-residents. The results provide insights into an under-researched subject, helping to highlight potential points of intervention for supporting public health programs within the boarding-school student population.
Shedding light on pediatric diseases: multispectral optoacoustic tomography at the doorway to clinical applications
Authors: Regensburger Adrian P. , Wagner Alexandra L. , Claussen Jing , Waldner Maximilian J. , Knieling Ferdinand
Published: March 2020

Optoacoustic imaging (OAI), or photoacoustic imaging (PAI), has fundamentally influenced basic science by providing high-resolution visualization of biological mechanisms. With the introduction of multispectral optoacoustic tomography (MSOT), these technologies have now moved closer to clinical applications. MSOT utilizes short-pulsed near-infrared laser light to induce thermoelastic expansion in targeted tissues. This results in acoustic pressure waves, which are used to resolve specific endo- and exogenous chromophores. Especially in the pediatric population, this non-invasive imaging approach might hold fundamental advantages compared to conventional cross-sectional imaging modalities. As this technology allows the visualization of quantitative molecular tissue composition at high spatial resolution non-invasively in sufficient penetration depth, it paves the way to personalized medicine in pediatric diseases.
Pediatric cerebellar malformations: magnetic resonance diagnostic merits and correlation with neurodevelopmental outcome
Authors: Ibrahim Rania S. M. , Hachem Rania H.
Published: March 2020

Background

In spite of having many classifications for pediatric cerebellar malformations (PCMs), no broadly accepted classification is recommended. Associated neurodevelopmental outcomes in children with PCMs remain poorly defined. Neuroimaging is compulsory for the diagnosis of cerebellar malformation and their associated abnormalities. This article emphasizes on the clinical and radiological traits of PCMs. It proposes a radiological classification and a diagnostic approach and assesses whether specific neuroimaging features in patients with PCM correlate with their neurodevelopmental outcomes.

Results

Fifty-eight pediatric patients were classified as follows: The majority of about 51 cases (88%) showed cerebellar hypoplasia and the remaining 7 cases (12%) showed cerebellar dysplasia. Twenty-six patients (45%) remained undiagnosed, while 32 patients (55%) were having a final diagnosis (24% Dandy-Walker malformation (DWM) (n = 14), 7% isolated vermian hypoplasia (n = 4), 7% congenital disorder of glycosylation (CDG) (n = 4), 5% congenital muscular dystrophy (n = 3), 5% congenital cytomegalovirus (CMV) infection (n = 30), 3% rhombencephalosynapsis (n = 2), 2% Lhermitte-Duclos syndrome (n = 1), and 2% DWM with Joubert syndrome (n = 1)). Overall, for the neurodevelopmental outcome, the majority of patients 90% (52/58) had a global developmental delay (GDD) which is a delay in two or more developmental domains. Both motor and language delay represented about 72% (37/58), intellectual disability was present in 59% (34/58), epilepsy in 53% (31/58), ataxic gait in 57% (33/58), attention deficit hyperactivity disorder (ADHD) in 19% (11/58), autism spectrum disorder (ASD) in 17% (10/58), nystagmus and tremors in 15% (9/58), and behavioral changes in 7% (6/58). Most of the children with cerebellar hypoplasia, about 93%, had GDD. Also, patients with PCH associated with a severe GDD, 75% had a language delay, 50% had intellectual and motor delay, and about 25% had epilepsy. However, we observed mild GDD in half of the vermian hypoplasia cases and half of them had a mild fine motor delay.

Conclusions

Magnetic resonance imaging (MRI) of the pediatric brain provides key information to categorize and classify cerebellar malformations. A neurodevelopmental deficit is highly associated with different types of PCMs. Severe GDD was associated with cerebellar and brain stem involvement. However, children with vermis mal-development were likely to have mild GDD. Familiarity with their diagnostic criteria is mandatory for correct diagnosis and prognosis for patients.
Integrated transmitter devices on InP exploiting electro-absorption modulation
Authors: Baier M. , Grote N. , Moehrle M. , Sigmund A. , Soares F. M. , Theurer M. , Troppenz U.
Published: March 2020

InP technology is the principal enabler for implementing fully monolithic photonic integrated circuits (PIC), uniquely including transmitter elements. In this article we present an overview of recent achievements on ultra-high speed electro-absorption modulated lasers (EML) which represent a simple transmitter PIC comprising a single-mode laser diode and an electro-absorption modulator. Using a so-called identical-layer approach single-wavelength modulation rates up to 100 Gb/s have been accomplished. By additionally integrating an optical amplifier section modulated optical output power of > 10 dBm has been achieved. Multi-level amplitude modulation was successfully demonstrated. Extended EML chips designed for wavelength-division and space-division multiplexing, respectively, will be presented. For dual-polarization transmission a novel EML related transmitter as well as a corresponding receiver PIC have been introduced. The latter devices were made on a generic PIC platform that is available for open-access foundry service.
The plant–pathogen haustorial interface at a glance
Authors: Bozkurt Tolga O. , Kamoun Sophien
Published: March 2020

Many filamentous pathogens invade plant cells through specialized hyphae called haustoria. These infection structures are enveloped by a newly synthesized plant-derived membrane called the extrahaustorial membrane (EHM). This specialized membrane is the ultimate interface between the plant and pathogen, and is key to the success or failure of infection. Strikingly, the EHM is reminiscent of host-derived membrane interfaces that engulf intracellular metazoan parasites. These perimicrobial interfaces are critical sites where pathogens facilitate nutrient uptake and deploy virulence factors to disarm cellular defenses mounted by their hosts. Although the mechanisms underlying the biogenesis and functions of these host–microbe interfaces are poorly understood, recent studies have provided new insights into the cellular and molecular mechanisms involved. In this Cell Science at a Glance and the accompanying poster, we summarize these recent advances with a specific focus on the haustorial interfaces associated with filamentous plant pathogens. We highlight the progress in the field that fundamentally underpin this research topic. Furthermore, we relate our knowledge of plant–filamentous pathogen interfaces to those generated by other plant-associated organisms. Finally, we compare the similarities between host–pathogen interfaces in plants and animals, and emphasize the key questions in this research area.
What Remains of Cross-Country Convergence?
Authors: Johnson Paul , Papageorgiou Chris
Published: March 2020

We examine the record of cross-country growth over the past fifty years and ask if developing countries have made progress on closing the income gap between their per capita incomes and those in the advanced economies. We conclude that, as a group, they have not and then survey the literature on absolute convergence with particular emphasis on that from the last decade or so. That literature supports our conclusion of a lack of progress in closing the income gap between countries. We close with a brief examination of the recent literature on cross-individual distribution of income, which finds that despite the lack of progress on cross country convergence, global inequality has tended to fall since 2000. ( JEL E01, E13, O11, O47, F41, F62)
What Students Learn in Economics 101: Time for a Change
Authors: Bowles Samuel , Carlin Wendy
Published: March 2020

We make the case for a shift in what students learn in a first economics course, taking as our exemplar Paul Samuelson’s paradigm-setting 1948 text. In the shadow of the Great Depression, Samuelson made Keynesian economics an essential component of what every economics student should know. By contrast, leading textbooks today were written in the glow of the Great Moderation and the tamed cyclical fluctuations in the two decades prior to 2007. Here, using topic modeling, we document Samuelson’s novelty and the evolution of the content of introductory textbooks since, and we put forward three propositions. First, as was the case in the aftermath of the Great Depression, new problems now challenge the content of our introductory courses; these include mounting inequalities, climate change, concerns about the future of work, and financial instability. Second, the tools required to address these problems, including strategic interaction, limited information, principal–agent models, new behavioral foundations, and dynamic processes including instability and path dependence, are available (indeed widely taught in PhD programs). And third, as we will illustrate by reference to a new open access introductory text, a course integrating these tools into a new benchmark model can be accessible, engaging, coherent and, as a result, successfully taught to first-year students. Deployed to address the new problems, following Samuelson’s example, the new benchmark provides the basis for integrating not only micro- and macroeconomics but also the analysis of both market failures and the limits of government interventions. ( JEL A22, D00, E00).
Reflections of a Textbook Author
Authors: Mankiw N. Gregory
Published: March 2020

In this essay, I reflect on textbook writing after three decades of participating in the activity. I address the following questions: What perspective should textbooks take? What is the best approach to teaching microeconomics? What is the best approach to teaching macroeconomics? How does the content of the introductory course evolve? How much material should textbooks include? Are textbooks too expensive? How is digital technology changing the market for textbooks? Who should become a textbook author? ( JEL A22)
Pleural, pancreatic and prostatic involvement in IgG4-related disease mimicking pancreatic head malignancy
Authors: Foley Robert W , Redman Stewart L , Graham Richard N , Phull Jaspal S , Masani Vidan , Colleypriest Benjamin J , Little David
Published: March 2020

We describe the case of a gentleman with pleural thickening. On follow-up imaging, dilatation of the main pancreatic and common biliary ducts was noted and an initial diagnosis of pancreatic malignancy was made. During his preoperative workup for pancreatic head malignancy, a PET-CT was performed, which demonstrated increased uptake in the pancreas, in the pleura and in the prostate gland. This raised the possibility of immunoglobulin G4-related disease (IgG4-RD), which was effectively treated with oral steroids. IgG4-RD is a well-described cause of autoimmune pancreatitis but can affect other regions, including the pleura and prostate. It is essential that radiologists are aware of the imaging findings in IgG4-RD and can direct clinicians towards this important multisystem diagnosis.
Differentially Private Distributed Learning
Authors: Zhou Yaqin , Tang Shaojie
Published: March 2020

The rich data used to train learning models increasingly tend to be distributed and private. It is important to efficiently perform learning tasks without compromising individual users’ privacy even considering untrusted learning applications and, furthermore, understand how privacy-preservation mechanisms impact the learning process. To address the problem, we design a differentially private distributed algorithm based on the stochastic variance reduced gradient (SVRG) algorithm, which prevents the learning server from accessing and inferring private training data with a theoretical guarantee. We quantify the impact of the adopted privacy-preservation measure on the learning process in terms of convergence rate, by which it indicates noises added at each gradient update results in a bounded deviation from the optimum. To further evaluate the impact on the trained models, we compare the proposed algorithm with SVRG and stochastic gradient descent using logistic regression and neural nets. The experimental results on benchmark data sets show that the proposed algorithm has minor impact on the accuracy of trained models under a moderate amount of privacy budget.
Exact MCMC for Choices from Menus—Measuring Substitution and Complementarity Among Menu Items
Authors: Kosyakova Tetyana , Otter Thomas , Misra Sanjog , Neuerburg Christian
Published: March 2020

We measure heterogeneous substitutive and complementary relationships between items in a menu sampling from the exact posterior of our model and showcase implications for optimal pricing.
White-light circular-polarization imaging using pairs of polarization gratings and wedge prisms
Authors: Noda Kohei , Yamada Koya , Momosaki Ryusei , Sakamoto Moritsugu , Sasaki Tomoyuki , Kawatsuki Nobuhiro , Tsutsui Kimiaki , Ono Hiroshi
Published: March 2020

A novel, to the best of our knowledge, method for circular-polarization imaging with white light is presented. The vital optical elements of the proposed system are two polarization gratings (PGs) and a wedge prism. The chromatic dispersion of the PG diffraction angle is compensated by the difference of the grating period for the two PGs. In addition, the distance between the diffracted lights on the imaging plane is controlled by using a wedge prism. As a result,

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3

of the Stokes parameter, including the wavelength dependence of a scarab beetle, was observed at a time, and the

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3

contribution to each color was determined by using the color filter of the light-receiving element. Because the white-light polarization image includes a lot of information about the illuminated structure, the proposed method should be applied to biomedical sensing and remote sensing.
Polarization contrast optical diffraction tomography
Authors: van Rooij Jos , Kalkman Jeroen
Published: March 2020

We demonstrate large scale polarization contrast optical diffraction tomography (ODT). In cross-polarized sample arm detection configuration we determine, from the amplitude of the optical wavefield, a relative measure of the birefringence projection. In parallel-polarized sample arm detection configuration we image the conventional phase projection. For off-axis sample placement we observe for polarization contrast ODT, similar as for phase contrast ODT, a strongly reduced noise contribution. In the limit of small birefringence phase shift δ we demonstrate tomographic reconstruction of polarization contrast images into a full 3D image of an optically cleared zebrafish. The polarization contrast ODT reconstruction shows muscular zebrafish tissue, which cannot be visualized in conventional phase contrast ODT. Polarization contrast ODT images of the zebrafish show a much higher signal to noise ratio (SNR) than the corresponding phase contrast images, SNR=73 and SNR=15, respectively.
Optical frequency comb noise spectra analysis using an asymmetric fiber delay line interferometer
Authors: Tian Haochen , Yang Wenkai , Kwon Dohyeon , Li Runmin , Zhao Yuwei , Kim Jungwon , Song Youjian , Hu Minglie
Published: March 2020

A simple and practical apparatus enabling repetition rate (frep) noise, carrier-envelope frequency (fceo) noise and nth optical comb mode (νn) noise spectra measurements with high precision is established. The frep and νn noise spectra are measured by a fiber delay line interferometer, while fceo noise spectrum is measured by an f-2f interferometer. We utilize this apparatus to characterize the noise performance of an Er-fiber optical frequency comb (OFC) and analyze the origin of dominant noise sources. Moreover, this apparatus provides a powerful tool for diagnosing noise dynamics intrinsic in mode-locked lasers and OFCs. To this end, we uncover the anti-correlation between frep and fceo noise as well as the impact of servo loops on noise characteristics in the stabilized OFC.
Strong coupling between distant photonic nanocavities via dark whispering gallery modes
Authors: Zhao Yanhui , Chen Li-Heng
Published: March 2020

The strong coupling between photonic nanocavities at arbitrary positions is important for the realization of photonic integrated circuits. However, the coupling between nanocavities is mainly through the evanescent field, which limits the distance between nanocavities and hinders the scalability of photonic circuits. Here, we propose a scheme to realize the strong coupling between two distant nanocavities beyond the limitations of evanescent field coupling. Two distant identical one dimensional photonic crystal cavities (1DPhCCs) more than 8 µm apart are bridged by a microring which supports whispering gallery modes (WGMs). We demonstrate that the two 1DPhCCs can be strongly coupled even though the microring is largely detuned from them. The supermodes between the two 1DPhCCs are formed while the proportions of the WGM in the microring are suppressed at large detuning. The light energy mainly oscillates between the two 1DPhCCs, leaving the WGM in the microring as a dark mode. Such a scheme can realize strong coupling between distant nanocavities without much difficulties in experiments, which provides advantages for the realization of next-generation photonic circuits.
Avaliando a re-expressão e criatividade de alunos de tradução
Authors: Bastin Georges L. , Esqueda Marileide Dias , Freitas Walter
Published: March 2020

Embora a tradução possa ser considerada um processo de comunicação que se realiza em duas (ou três) fases, isto é, compreensão – (conceituação) – e re-expressão, a maioria dos estudos teóricos e pedagógicos tem se dedicado à compreensão e conceituação. Há, no entanto, uma necessidade crescente de estabelecer uma base teórica para a terceira fase, uma vez que, contrariamente à máxima de Boileau (de que ideias bem concebidas podem ser facilmente expressadas), mesmo quando há compreensão total, as palavras não surgem facilmente. Para que a re-expressão seja mais bem ensinada, sua avaliação precisa ser repensada. Este artigo concentra-se na avaliação da re-expressão em tradução. Com base em um estudo detalhado de vários textos escritos em inglês e traduzidos para o francês por cerca de 38 alunos do primeiro ano de um curso de Tradução, primeiramente ressalta a diferença entre expressão e re-expressão e entre criatividade e literalidade, sendo aquela vista como um “desvio” (ou afastamento) desta. Em segundo lugar, defende uma avaliação positiva (que envolve a análise de soluções bem-sucedidas, e não de erros), já que a avaliação negativa tem um impacto relativamente limitado no processo de aprendizagem e estudá-la não seria muito produtivo.
The First Translation of Patrick White’s The Solid Mandala into Spanish
Authors: Stefani Mônica
Published: March 2020

This paper analyses some aspects (use of footnotes, intertextuality, punctuation and maintenance of cultural elements) of Las esferas del mandala, the first Spanish translation (by Silvia Pupato and Román García-Azcárate and published in Barcelona in 1973) of The Solid Mandala, written by the Australian Nobel Prize winner Patrick White in 1966. Through the selection of excerpts from the original considered problematic to be rendered in translation, we observe the solutions found, as well as some strategies adopted by the Spanish translators to compose the final product presented to the readers. This contrastive reading hopes to engender interesting ideas to help future translators of the novel, while valuing the translation act.
MICROSTRUCTURE INVESTIGATION OF SEAWATER VS. FRESHWATER CEMENT PASTES
Authors: Younis Adel , Ebead Usama , Suraneni Prannoy , Nanni Antonio
Published: March 2020

Recently, seawater has emerged as viable mixing water for concrete, especially in the case of non-reinforced concrete applications or with the use of non-corrosive reinforcement. Previous studies concerning seawater-mixed concrete mostly revealed an initial slight increase in its strength performance (i.e., till Day 14 following mixing), followed by a strength reduction of 7–15% (i.e., after 28 days or longer) as compared to the conventional freshwater-mixed concrete. With an attempt to explain such observations, this paper aims at comparing the microstructure of freshwater-and seawater-mixed cement pastes. Scanning electron microscopy was utilized to observe the microstructure of freshwater and seawater pastes at Days 3 and 28 following mixing. At Day 3, seawater paste was observed to have more densified microstructure as compared to that of the freshwater counterpart, resulting in relatively higher strength performance. At Day 28, the microstructure was almost similar for the two cement pastes. However, seawater paste was observed to have salt impurities as a result of seawater ions, which possibly cause a slightly lower strength performance as compared to the freshwater paste.
MODEL DISASTER PREVENTION LESSONS IN PRIMARY SCHOOL BASED ON DISASTER PREVENTION EDUCATION POLICIES
Authors: Imura Noriko , Ishikawa Takashige
Published: March 2020

This study deals with disaster mitigation education in primary schools and as a final goal aims to improve citizens’ capabilities to mitigate disaster situations in society. This report summarizes the current national policy on safety education in Japan and follow it up with a summarization of the Tokyo Metropolitan Government policy, which is based on the national policy. In addition, it compares Japan’s education policy with that of New Zealand. Analysis revealed the following three points. First, the content of safety education in Japanese primary schools consists of six fields (traffic safety, daily life safety, disaster safety, etc.). Just before, and after, the Great Tohoku Earthquake in 2011, the main educational content changed from daily life safety to disaster safety. Second, by focusing on lessons, it was found that the content of disaster safety was taught not as part of various subjects but largely during “homeroom activities”. Third, it became clear that the subject of earthquakes now accounts for half of the disaster prevention lesson contents in Japanese primary schools and has been included in disaster prevention lesson contents in all school grades.
AMASS: ADVANCED MANUFACTURING FOR THE ASSEMBLY OF STRUCTURAL STEEL
Authors: Schultz Arturo E. , Le Jia-Liang , Shemshadian Mohamed E. , Labbane Ramzi , Laefer Debra F. , Al-Sabah Salam , Truong-Hong Linh , Huynh Minh , McGetrick Patrick , Martin Tony , Matis Pantelis
Published: March 2020

This paper describes a collaborative project between the US, Ireland, and Northern Ireland (UK) to investigate advanced manufacturing cutting techniques for the creation of a new class of intermeshed steel connections that rely on neither welding nor bolting. To date, advanced manufacturing equipment has only been used to accelerate traditional processes for cutting sheet metal or other conventional fabrication activities. Such approaches have not capitalized on the equipment's full potential. This project lays the groundwork to transform the steel building construction industry by investigating the underlying science and engineering precepts for intermeshed connections created from precise, volumetric cutting. The proposed system enhances the integration between design, fabrication, installation and maintenance through building information modeling platforms to implement advanced connections. Fully automated, precise, volumetric cutting of open steel sections introduces intellectual challenges regarding the load-transfer mechanisms and failure modes for intermeshed connections. The research activity addresses knowledge gaps concerning the load resistance and design of steel systems with intermeshed connections. Physical tests, finite element simulation and multi-scale modeling are being used to investigate the mechanics of intermeshed connections including stress and strain concentrations, fracture potential and failure modes, and to optimize connection geometry.