bluuebunny/crossref_metadata_2025 huggingface dataset:
Building collaboration in teams through emotional intelligence: Mediation by SOAR (strengths, opportunities, aspirations, and results)
Authors: Cole Matthew L , Cox John D , Stavros Jacqueline M
Published: November 2016

In today’s global business environment teams are fast becoming the norm. Collaboration is an essential factor in leveraging team effectiveness, and organizations are looking for strategies to increase collaboration among their teams. In this study, we administered an eSurvey to 308 professionals working in face-to-face and virtual teams to investigate emotional intelligence and strengths, opportunities, aspirations, and results as strategies to support the collaborative process. Results found the regression of collaboration on emotional intelligence (controlling for age, ethnicity, and education) was significant (p<.01). Results also found a significant indirect effect between emotional intelligence and collaboration as mediated by strengths, opportunities, aspirations, and results (β=0.110,Z=2.444). We focus on understanding the effect of emotional intelligence on team collaboration as mediated by strengths, opportunities, aspirations, and results. Recommendations are provided for increasing emotional intelligence and strengths, opportunities, aspirations, and results among team members. Our research has important implications for teams and their pervasive use in business.
Brokerage-based attack on real world temporal networks
Authors: SUR SOUVIK , GANGULY NILOY , MUKHERJEE ANIMESH
Published: November 2016

In this paper, we attempt to investigate the attack tolerance of human mobility networks where the mobility is restricted to some extent, for instance, in a hospital, one is not allowed to access all locations. Similar situations also arise in schools. In such a network, we will show that people need to rely upon some intermediate agents, popularly known as thebrokersto disseminate information. In order to establish this fact, we have followed the approach of attack in a network which in turn helps to identify important nodes in the network in order to maintain the overall connectivity. In this direction, we have proposed, a new temporal metric,brokerage frequencywhich significantly outperforms all other state-of-the-art attack strategies reported in Trajanovski et al. (2012), Sur et al. (2015).
Nutrigenomics in the modern era
Authors: Mathers John C.
Published: November 2016

The concept that interactions between nutrition and genetics determine phenotype was established by Garrod at the beginning of the 20th century through his ground-breaking work on inborn errors of metabolism. A century later, the science and technologies involved in sequencing of the human genome stimulated development of the scientific discipline which we now recognise as nutritional genomics (nutrigenomics). Much of the early hype around possible applications of this new science was unhelpful and raised expectations, which have not been realised as quickly as some would have hoped. However, major advances have been made in quantifying the contribution of genetic variation to a wide range of phenotypes and it is now clear that for nutrition-related phenotypes, such as obesity and common complex diseases, the genetic contribution made by SNP alone is often modest. There is much scope for innovative research to understand the roles of less well explored types of genomic structural variation, e.g. copy number variants, and of interactions between genotype and dietary factors, in phenotype determination. New tools and models, including stem cell-based approaches and genome editing, have huge potential to transform mechanistic nutrition research. Finally, the application of nutrigenomics research offers substantial potential to improve public health e.g. through the use of metabolomics approaches to identify novel biomarkers of food intake, which will lead to more objective and robust measures of dietary exposure. In addition, nutrigenomics may have applications in the development of personalised nutrition interventions, which may facilitate larger, more appropriate and sustained changes in eating (and other lifestyle) behaviours and help to reduce health inequalities.
Words are not enough: how preschoolers’ integration of perspective and emotion informs their referential understanding
Authors: GRAHAM SUSAN A. , SAN JUAN VALERIE , KHU MELANIE
Published: November 2016

When linguistic information alone does not clarify a speaker's intended meaning, skilled communicators can draw on a variety of cues to infer communicative intent. In this paper, we review research examining the developmental emergence of preschoolers’ sensitivity to a communicative partner's perspective. We focus particularly on preschoolers’ tendency to use cues both within the communicative context (i.e. a speaker's visual access to information) and within the speech signal itself (i.e. emotional prosody) to make on-line inferences about communicative intent. Our review demonstrates that preschoolers’ ability to use visual and emotional cues of perspective to guide language interpretation is not uniform across tasks, is sometimes related to theory of mind and executive function skills, and, at certain points of development, is only revealed by implicit measures of language processing.
A process evaluation of a Psychomotor Dance Therapy Intervention (DANCIN) for behavior change in dementia: attitudes and beliefs of participating residents and staff
Authors: Guzmán Azucena , Robinson Lisa , Rochester Lynn , James Ian A. , Hughes Julian C.
Published: November 2016

Background:

In a previous paper, we presented results from a 12-week study of a Psychomotor DANCe Therapy INtervention (DANCIN) based on Danzón Latin Ballroom that involves motor, emotional-affective, and cognitive domains, using a multiple-baseline single-case design in three care homes. This paper reports the results of a complementary process evaluation to elicit the attitudes and beliefs of home care staff, participating residents, and family members with the aim of refining the content of DANCIN in dementia care.

Methods:

An external researcher collected bespoke questionnaires from ten participating residents, 32 care home staff, and three participants’ family members who provided impromptu feedback in one of the care homes. The Behavior Change Technique Taxonomy v1 (BCTTv1) provided a methodological tool for identifying active components of the DANCIN approach warranting further exploration, development, and implementation.

Results:

Ten residents found DANCIN beneficial in terms of mood and socialization in the care home. Overall, 78% of the staff thought DANCIN led to improvements in residents’ mood; 75% agreed that there were improvements in behavior; 56% reported increased job satisfaction; 78% of staff were enthusiastic about receiving further training. Based on participants’ responses, four BCTTv1 labels–Social support (emotional), Focus on past success and verbal persuasion to boost self-efficacy, Restructuring the social environment and Habit formation–were identified to describe the intervention. Residents and staff recommended including additional musical genres and extending the session length. Discussions of implementing a supervision system to sustain DANCIN regularly regardless of management or staff turnover were suggested.

Conclusions:

Care home residents with mild to moderate dementia wanted to continue DANCIN as part of their routine care and staff and family members were largely supportive of this approach. This study argues in favor of further dissemination of DANCIN in care homes. We provide recommendations for the future development of DANCIN based on the views of key stakeholder groups.
The economic burden of inadequate consumption of vegetables and fruit in Canada
Authors: Ekwaru John Paul , Ohinmaa Arto , Loehr Sarah , Setayeshgar Solmaz , Thanh Nguyen Xuan , Veugelers Paul J
Published: November 2016

Objective

Public health decision makers not only consider health benefits but also economic implications when articulating and issuing lifestyle recommendations. Whereas various estimates exist for the economic burden of physical inactivity, excess body weight and smoking, estimates of the economic burden associated with our diet are rare. In the present study, we estimated the economic burden attributable to the inadequate consumption of vegetables and fruit in Canada.

Design

We accessed the Canadian Community Health Survey to assess the inadequacy in the consumption of vegetables and fruit and published meta-analyses to assemble risk estimates for chronic diseases. Based on these inadequacy and risk estimates, we calculated the population-attributable fraction and avoidable direct and indirect costs to society. Direct costs include those for hospital care, physician services and drugs in 2015.

Results

About 80 % of women and 89 % of men consume inadequate amounts of vegetables and fruit. We estimated this to result in an economic burden of $CAN 3·3 billion per year, of which 30·5 % is direct health-care costs and 69·5 % is indirect costs due to productivity losses. A modest 1 percentage point annual reduction in the prevalence of inadequate vegetables and fruit consumption over the next 20 years would avoid approximately $CAN 10·8 billion, and an increase of one serving of vegetables and fruit per day would avoid approximately $CAN 9·2 billion.

Conclusions

Further investments in the promotion of vegetables and fruit will prevent chronic disease and substantially reduce direct and indirect health-care costs.
Semi-automated operation of Mars Climate Simulation chamber – MCSC modelled for biological experiments
Authors: Tarasashvili M.V. , Sabashvili Sh.A. , Tsereteli S.L. , Aleksidze N.D. , Dalakishvili O.
Published: November 2016

The Mars Climate Simulation Chamber (MCSC) (GEO PAT 12 522/01) is designed for the investigation of the possible past and present habitability of Mars, as well as for the solution of practical tasks necessary for the colonization and Terraformation of the Planet. There are specific tasks such as the experimental investigation of the biological parameters that allow many terrestrial organisms to adapt to the imitated Martian conditions: chemistry of the ground, atmosphere, temperature, radiation, etc. MCSC is set for the simulation of the conduction of various biological experiments, as well as the selection of extremophile microorganisms for the possible Settlement, Ecopoesis and/or Terraformation purposes and investigation of their physiological functions. For long-term purposes, it is possible to cultivate genetically modified organisms (e.g., plants) adapted to the Martian conditions for future Martian agriculture to sustain human Mars missions and permanent settlements. The size of the chamber allows preliminary testing of the functionality of space-station mini-models and personal protection devices such as space-suits, covering and building materials and other structures. The reliability of the experimental biotechnological materials can also be tested over a period of years. Complex and thorough research has been performed to acquire the most appropriate technical tools for the accurate engineering of the MCSC and precious programmed simulation of Martian environmental conditions. This paper describes the construction and technical details of the equipment of the MCSC, which allows its semi-automated, long-term operation.
Parental experiences with a hospital-based bereavement program following the loss of a child to cancer
Authors: Berrett-Abebe Julie , Levin-Russman Elyse , Gioiella Marie Elena , Adams Jeffrey M.
Published: November 2016

Objective:

The death of a child from cancer is an intense and life-changing loss for a parent. Guided by the principles of patient- and family-centered care, hospital-based caregivers developed a program to provide bereavement support for parents through phone calls and mailings. The aim of the present qualitative phenomenological study was to understand how parents experienced participating in this bereavement program.

Method:

A total of eight parents from six families participated in a focus-group evaluation of the two-year hospital-based bereavement program. Two social work clinicians/researchers independently analyzed the transcript of the focus group to define themes.

Results:

Four themes were identified: (1) lived experience of grief, (2) importance of relationships with the hospital-based team, (3) bereavement support from hospital-based providers, and (4) extending bereavement care.

Significance of Results:

Participants indicated the value of ongoing communication and connection with members of the healthcare team, who were often central to a family's life for years during their child's cancer treatment. Parents also provided suggestions for extending bereavement support through continued contact with providers and informal annual gatherings, as well as through a peer (parent-to-parent) support program.
RACE, JUSTICE, POLICING, AND THE 2016 AMERICAN PRESIDENTIAL ELECTION
Authors: Drakulich Kevin , Hagan John , Johnson Devon , Wozniak Kevin H.
Published: November 2016

Scholars have long been interested in the intersection of race, crime, justice, and presidential politics, focusing particularly on the “southern strategy” and the “war on crime.” A recent string of highly-publicized citizen deaths at the hands of police and the emergence of the Black Lives Matter movement have brought renewed visibility to this racially-driven intersection, and in particular to issues involving contact with and attitudes toward the police. Using data from the 2016 Pilot Study of the American National Election Studies, this study explores how contact with the criminal justice system and perceptions of police injustice shape political behavior in the modern era, with a specific emphasis on prospective participation and candidate choice in the 2016 presidential election. The results indicate that being stopped by the police—an experience that can feel invasive and unjust—may motivate political participation, while spending time in jail or prison—an experience associated with a marginalization from mainstream civic life—appears to discourage political participation. Perceiving the police as discriminatory also seems to motivate political engagement and participation, though in opposite directions for conservative versus liberal voters. In addition, perceptions of police injustice were related to candidate choice, driving voters away from Donald Trump. Affective feelings about the police were not associated with candidate choice. Perceptions of the police appear to act in part as a proxy for racial resentments, at least among potential voters in the Republican primary. In sum, the intersection of race, justice, and policing remains highly relevant in U.S. politics.
Large-scale self-organization of reconfigurable topological defect networks in nematic liquid crystals
Authors: Sasaki Yuji , Jampani V.S.R. , Tanaka Chiharu , Sakurai Nobutaka , Sakane Shin , Le Khoa V. , Araoka Fumito , Orihara Hiroshi
Published: November 2016

Topological defects in nematic liquid crystals are ubiquitous. The defects are important in understanding the fundamental properties of the systems, as well as in practical applications, such as colloidal self-assembly, optical vortex generation and templates for molecular self-assembly. Usually, spatially and temporally stable defects require geometrical frustration imposed by surfaces; otherwise, the system relaxes because of the high cost of the elastic energy. So far, multiple defects are kept in bulk nematic liquid crystals by top-down lithographic techniques. In this work, we stabilize a large number of umbilical defects by doping with an ionic impurity. This method does not require pre-patterned surfaces. We demonstrate that molecular reorientation controlled by an AC voltage induces periodic density modulation of ions accumulated at an electrically insulating polymer interface, resulting in self-organization of a two-dimensional square array of umbilical defects that is reconfigurable and tunable.
Genome-wide association study of 12 agronomic traits in peach
Authors: Cao Ke , Zhou Zhengkui , Wang Qi , Guo Jian , Zhao Pei , Zhu Gengrui , Fang Weichao , Chen Changwen , Wang Xinwei , Wang Xiaoli , Tian Zhixi , Wang Lirong
Published: November 2016

Peach (Prunus persica L.) is a highly valuable crop species and is recognized by molecular researchers as a model fruit for the Rosaceae family. Using whole-genome sequencing data generated from 129 peach accessions, here we perform a comprehensive genome-wide association study for 12 key agronomic traits. We show that among the 10 qualitative traits investigated, nine exhibit consistent and more precise association signals than previously identified by linkage analysis. For two of the qualitative traits, we describe candidate genes, one potentially involved in cell death and another predicted to encode an auxin-efflux carrier, that are highly associated with fruit shape and non-acidity, respectively. Furthermore, we find that several genomic regions harbouring association signals for fruit weight and soluble solid content overlapped with predicted selective sweeps that occurred during peach domestication and improvement. Our findings contribute to the large-scale characterization of genes controlling agronomic traits in peach.
Excitonic linewidth and coherence lifetime in monolayer transition metal dichalcogenides
Authors: Selig Malte , Berghäuser Gunnar , Raja Archana , Nagler Philipp , Schüller Christian , Heinz Tony F. , Korn Tobias , Chernikov Alexey , Malic Ermin , Knorr Andreas
Published: November 2016

Atomically thin transition metal dichalcogenides are direct-gap semiconductors with strong light–matter and Coulomb interactions. The latter accounts for tightly bound excitons, which dominate their optical properties. Besides the optically accessible bright excitons, these systems exhibit a variety of dark excitonic states. They are not visible in the optical spectra, but can strongly influence the coherence lifetime and the linewidth of the emission from bright exciton states. Here, we investigate the microscopic origin of the excitonic coherence lifetime in two representative materials (WS2 and MoSe2) through a study combining microscopic theory with spectroscopic measurements. We show that the excitonic coherence lifetime is determined by phonon-induced intravalley scattering and intervalley scattering into dark excitonic states. In particular, in WS2, we identify exciton relaxation processes involving phonon emission into lower-lying dark states that are operative at all temperatures.
Observation of the spin-polarized surface state in a noncentrosymmetric superconductor BiPd
Authors: Neupane Madhab , Alidoust Nasser , Hosen M. Mofazzel , Zhu Jian-Xin , Dimitri Klauss , Xu Su-Yang , Dhakal Nagendra , Sankar Raman , Belopolski Ilya , Sanchez Daniel S. , Chang Tay-Rong , Jeng Horng-Tay , Miyamoto Koji , Okuda Taichi , Lin Hsin , Bansil Arun , Kaczorowski Dariusz , Chou Fangcheng , Hasan M. Zahid , Durakiewicz Tomasz
Published: November 2016

Recently, noncentrosymmetric superconductor BiPd has attracted considerable research interest due to the possibility of hosting topological superconductivity. Here we report a systematic high-resolution angle-resolved photoemission spectroscopy (ARPES) and spin-resolved ARPES study of the normal state electronic and spin properties of BiPd. Our experimental results show the presence of a surface state at higher-binding energy with the location of Dirac point at around 700 meV below the Fermi level. The detailed photon energy, temperature-dependent and spin-resolved ARPES measurements complemented by our first-principles calculations demonstrate the existence of the spin-polarized surface states at high-binding energy. The absence of such spin-polarized surface states near the Fermi level negates the possibility of a topological superconducting behaviour on the surface. Our direct experimental observation of spin-polarized surface states in BiPd provides critical information that will guide the future search for topological superconductivity in noncentrosymmetric materials.
Phosphorene/rhenium disulfide heterojunction-based negative differential resistance device for multi-valued logic
Authors: Shim Jaewoo , Oh Seyong , Kang Dong-Ho , Jo Seo-Hyeon , Ali Muhammad Hasnain , Choi Woo-Young , Heo Keun , Jeon Jaeho , Lee Sungjoo , Kim Minwoo , Song Young Jae , Park Jin-Hong
Published: November 2016

Recently, negative differential resistance devices have attracted considerable attention due to their folded current–voltage characteristic, which presents multiple threshold voltage values. Because of this remarkable property, studies associated with the negative differential resistance devices have been explored for realizing multi-valued logic applications. Here we demonstrate a negative differential resistance device based on a phosphorene/rhenium disulfide (BP/ReS2) heterojunction that is formed by type-III broken-gap band alignment, showing high peak-to-valley current ratio values of 4.2 and 6.9 at room temperature and 180 K, respectively. Also, the carrier transport mechanism of the BP/ReS2 negative differential resistance device is investigated in detail by analysing the tunnelling and diffusion currents at various temperatures with the proposed analytic negative differential resistance device model. Finally, we demonstrate a ternary inverter as a multi-valued logic application. This study of a two-dimensional material heterojunction is a step forward toward future multi-valued logic device research.
RETRACTED ARTICLE: Culture of previously uncultured members
of the human gut microbiota by culturomics
Authors: Lagier Jean-Christophe , Khelaifia Saber , Alou Maryam Tidjani , Ndongo Sokhna , Dione Niokhor , Hugon Perrine , Caputo Aurelia , Cadoret Frédéric , Traore Sory Ibrahima , Seck El Hadji , Dubourg Gregory , Durand Guillaume , Mourembou Gaël , Guilhot Elodie , Togo Amadou , Bellali Sara , Bachar Dipankar , Cassir Nadim , Bittar Fadi , Delerce Jérémy , Mailhe Morgane , Ricaboni Davide , Bilen Melhem , Dangui Nieko Nicole Prisca Makaya , Dia Badiane Ndeye Mery , Valles Camille , Mouelhi Donia , Diop Khoudia , Million Matthieu , Musso Didier , Abrahão Jônatas , Azhar Esam Ibraheem , Bibi Fehmida , Yasir Muhammad , Diallo Aldiouma , Sokhna Cheikh , Djossou Felix , Vitton Véronique , Robert Catherine , Rolain Jean Marc , La Scola Bernard , Fournier Pierre-Edouard , Levasseur Anthony , Raoult Didier
Published: November 2016

Metagenomics revolutionized the understanding of the relations among
the human microbiome, health and diseases, but generated a countless number of
sequences that have not been assigned to a known
microorganism1. The pure culture of prokaryotes, neglected in
recent decades, remains essential to elucidating the role of these
organisms2. We recently introduced microbial culturomics, a
culturing approach that uses multiple culture conditions and matrix-assisted laser
desorption/ionization–time of flight and 16S rRNA for
identification2. Here, we have selected the best culture conditions
to increase the number of studied samples and have applied new protocols
(fresh-sample inoculation; detection of microcolonies and specific cultures of
Proteobacteria and microaerophilic and halophilic prokaryotes) to address the
weaknesses of the previous studies3–5. We
identified 1,057 prokaryotic species, thereby adding 531 species to the human gut
repertoire: 146 bacteria known in humans but not in the gut, 187 bacteria and 1
archaea not previously isolated in humans, and 197 potentially new species. Genome
sequencing was performed on the new species. By comparing the results of the
metagenomic and culturomic analyses, we show that the use of culturomics allows the
culture of organisms corresponding to sequences previously not assigned. Altogether,
culturomics doubles the number of species isolated at least once from the human
gut.
The role of master clock stability in quantum information processing
Authors: Ball Harrison , Oliver William D , Biercuk Michael J
Published: November 2016

Experimentalists seeking to improve the coherent lifetimes of quantum bits have generally focused on mitigating decoherence mechanisms through, for example, improvements to qubit designs and materials, and system isolation from environmental perturbations. In the case of the phase degree of freedom in a quantum superposition, however, the coherence that must be preserved is not solely internal to the qubit, but rather necessarily includes that of the qubit relative to the ‘master clock’ (e.g., a local oscillator) that governs its control system. In this manuscript, we articulate the impact of instabilities in the master clock on qubit phase coherence and provide tools to calculate the contributions to qubit error arising from these processes. We first connect standard oscillator phase-noise metrics to their corresponding qubit dephasing spectral densities. We then use representative lab-grade and performance-grade oscillator specifications to calculate operational fidelity bounds on trapped-ion and superconducting qubits with relatively slow and fast operation times. We discuss the relevance of these bounds for quantum error correction in contemporary experiments and future large-scale quantum information systems, and consider potential means to improve master clock stability.
Spatial and temporal dynamics of multidimensional well-being, livelihoods and ecosystem services in coastal Bangladesh
Authors: Adams Helen , Adger W. Neil , Ahmad Sate , Ahmed Ali , Begum Dilruba , Lázár Attila N. , Matthews Zoe , Rahman Mohammed Mofizur , Streatfield Peter Kim
Published: November 2016

Populations in resource dependent economies gain well-being from the natural environment, in highly spatially and temporally variable patterns. To collect information on this, we designed and implemented a 1586-household quantitative survey in the southwest coastal zone of Bangladesh. Data were collected on material, subjective and health dimensions of well-being in the context of natural resource use, particularly agriculture, aquaculture, mangroves and fisheries. The questionnaire included questions on factors that mediate poverty outcomes: mobility and remittances; loans and micro-credit; environmental perceptions; shocks; and women’s empowerment. The data are stratified by social-ecological system to take into account spatial dynamics and the survey was repeated with the same respondents three times within a year to incorporate seasonal dynamics. The dataset includes blood pressure measurements and height and weight of men, women and children. In addition, the household listing includes basic data on livelihoods and income for approximately 10,000 households. The dataset facilitates interdisciplinary research on spatial and temporal dynamics of well-being in the context of natural resource dependence in low income countries.
A variant reference data set for the Africanized honeybee, Apis mellifera
Authors: Kadri Samir M. , Harpur Brock A. , Orsi Ricardo O. , Zayed Amro
Published: November 2016

The Africanized honeybee (AHB) is a population of Apis mellifera found in the Americas. AHBs originated in 1956 in Rio Clara, Brazil where imported African A. m. scutellata escaped and hybridized with local populations of European A. mellifera. Africanized populations can now be found from Northern Argentina to the Southern United States. AHBs—often referred to as ‘Killer Bees’— are a major concern to the beekeeping industry as well as a model for the evolutionary genetics of colony defence. We performed high coverage pooled-resequencing of 360 diploid workers from 30 Brazilian AHB colonies using Illumina Hi-Seq (150 bp PE). This yielded a high density SNP data set with an average read depth at each site of 20.25 reads. With 3,606,720 SNPs and 155,336 SNPs within 11,365 genes, this data set is the largest genomic resource available for AHBs and will enable high-resolution studies of the population dynamics, evolution, and genetics of this successful biological invader, in addition to facilitating the development of SNP-based tools for identifying AHBs.
Cross-cultural dataset for the evolution of religion and morality project
Authors: Purzycki Benjamin Grant , Apicella Coren , Atkinson Quentin D. , Cohen Emma , McNamara Rita Anne , Willard Aiyana K. , Xygalatas Dimitris , Norenzayan Ara , Henrich Joseph
Published: November 2016

A considerable body of research cross-culturally examines the evolution of religious traditions, beliefs and behaviors. The bulk of this research, however, draws from coded qualitative ethnographies rather than from standardized methods specifically designed to measure religious beliefs and behaviors. Psychological data sets that examine religious thought and behavior in controlled conditions tend to be disproportionately sampled from student populations. Some cross-national databases employ standardized methods at the individual level, but are primarily focused on fully market integrated, state-level societies. The Evolution of Religion and Morality Project sought to generate a data set that systematically probed individual level measures sampling across a wider range of human populations. The set includes data from behavioral economic experiments and detailed surveys of demographics, religious beliefs and practices, material security, and intergroup perceptions. This paper describes the methods and variables, briefly introduces the sites and sampling techniques, notes inconsistencies across sites, and provides some basic reporting for the data set.
The role of gibberellins in improving the resistance of tebuconazole-coated maize seeds to chilling stress by microencapsulation
Authors: Yang Lijuan , Yang Daibin , Yan Xiaojing , Cui Li , Wang Zhenying , Yuan Huizhu
Published: November 2016

Chilling stress during germination often causes severe injury. In the present study, maize seed germination and shoot growth under chilling stress were negatively correlated with the dose of tebuconazole in an exponential manner as predicted by the model Y = A + B × e(−x/k). Microencapsulation was an effective means of eliminating potential phytotoxic risk. The gibberellins (GAs) contents were higher after microencapsulation treatment than after conventional treatment when the dose of tebuconazole was higher than 0.12 g AI (active ingredient) kg−1 seed. Further analysis indicated that microencapsulation can stimulate ent-kaurene oxidase (KO) activity to some extent, whereas GA 3-oxidase (GA3ox) and GA 2-oxidase (GA2ox) activities remained similar to those in the control. Genes encoding GA metabolic enzymes exhibited different expression patterns. Transcript levels of ZmKO1 increased in the microcapsule treatments compared to the control. Even when incorporated into microcapsules, tebuconazole led to the upregulation of ZmGA3ox1 at doses of less than 0.12 g AI kg−1 seed and to the upregulation of ZmGA3ox2 when the dose was higher than 0.12 g AI kg−1 seed. With increasing doses of microencapsulated tebuconazole, the transcript levels of ZmGA2ox4, ZmGA2ox5 and ZmGA2ox6 exhibited upward trends, whereas the transcript levels of ZmGA2ox7 exhibited a downward trend.
Performance Improvement of AlN Crystal Quality Grown on Patterned Si(111) Substrate for Deep UV-LED Applications
Authors: Tran Binh Tinh , Maeda Noritoshi , Jo Masafumi , Inoue Daishi , Kikitsu Tomoka , Hirayama Hideki
Published: November 2016

An AlN template layer is required for growth of AlGaN-based deep ultraviolet light-emitting diodes (UV-LEDs). However, the crystal quality of AlN templates grown on both flat and patterned Si substrates has so far been insufficient for replacing templates grown on sapphire substrates. In this work, we grew a high-quality AlN template on 2 in. micro-circle-patterned Si substrate (mPSiS) with two different sizes and shapes through controlling the bias power of inductively coupled plasma (ICP) etching. The experimental results showed that the best AlN template was obtained on a large pattern size with a bow-angle shape and the template had X-ray rocking curves with full widths at half-maximum of 620 and 1141 arcsec for the (002) and (102) reflection planes. The threading dislocation density near surface of AlN template through transmission electron microscopy (TEM) estimation was in the order of 107 cm−2, which is the lowest dislocation density reported for a Si substrate to our knowledge. A strong single electroluminescence (EL) peak was also obtained for an AlGaN-based deep UV-LED grown on this template, means that it can be used for further developing high-efficiency deep UV-LEDs.
Optogenetic activation of serotonergic terminals facilitates GABAergic inhibitory input to orexin/hypocretin neurons
Authors: Chowdhury Srikanta , Yamanaka Akihiro
Published: November 2016

Orexin/hypocretin neurons play a crucial role in the regulation of sleep/wakefulness, primarily in the maintenance of wakefulness. These neurons innervate wide areas of the brain and receive diverse synaptic inputs including those from serotonergic (5-HT) neurons in the raphe nucleus. Previously we showed that pharmacological application of 5-HT directly inhibited orexin neurons via 5-HT1A receptors. However, it was still unclear how 5-HT neurons regulated orexin neurons since 5-HT neurons contain not only 5-HT but also other neurotransmitters. To reveal this, we generated new triple transgenic mice in which orexin neurons express enhanced green fluorescent protein (EGFP) and 5-HT neurons express channelrhodopsin2 (ChR2). Immunohistochemical studies show that nerve endings of ChR2-expressing 5-HT neurons are in close apposition to EGFP-expressing orexin neurons in the lateral hypothalamic area. Using these mice, we could optogenetically activate 5-HT nerve terminals and record postsynaptic effects from orexin neurons. Activation of nerve terminals of 5-HT neurons directly inhibited orexin neurons via the 5HT1A receptor, and also indirectly inhibited orexin neurons by facilitating GABAergic inhibitory inputs without affecting glutamatergic inputs. Increased GABAergic inhibitory inputs in orexin neurons were confirmed by the pharmacological application of 5-HT. These results suggest that orexin neurons are inhibited by 5-HT neurons, primarily via 5-HT, in both direct and indirect manners.
Variant responses of tree seedling to seasonal drought stress along an elevational transect in tropical montane forests
Authors: Song Xiaoyang , Li Jieqiong , Zhang Wenfu , Tang Yong , Sun Zhenhua , Cao Min
Published: November 2016

Seasonal drought is a common phenomenon in many forests predominated by monsoon climate. The impact of seasonal drought, however, may vary with elevations, and tree species of forests hence may differ in their response to elevations. In this study, we monitored the seasonal variation of seedling species composition, and their relative growth rate (RGR) along an elevational transect in tropical forests of southwest China for two years. We found tree seedling species richness declined with rising elevation. Seedling abundance and species richness increased significantly from dry season to rainy season. In dry season, RGR declined progressively from low to high elevational bands, while positive RGR occurred in each elevation in rainy season. We grouped seedling species into low and high elevation specialists based on their elevational distributions. Seasonal variance in soil moisture may lead to seasonal dynamics of seedling community in this area. Our results suggest that the observed change in local climate over the last 40 years tends to allow the tree species from high elevation to expand their distribution to the lower elevation, while the ranges of those at low elevations could be compressed or at the risk of extinction.
Multi-label ℓ2-regularized logistic regression for predicting activation/inhibition relationships in human protein-protein interaction networks
Authors: Mei Suyu , Zhang Kun
Published: November 2016

Protein-protein interaction (PPI) networks are naturally viewed as infrastructure to infer signalling pathways. The descriptors of signal events between two interacting proteins such as upstream/downstream signal flow, activation/inhibition relationship and protein modification are indispensable for inferring signalling pathways from PPI networks. However, such descriptors are not available in most cases as most PPI networks are seldom semantically annotated. In this work, we extend ℓ2-regularized logistic regression to the scenario of multi-label learning for predicting the activation/inhibition relationships in human PPI networks. The phenomenon that both activation and inhibition relationships exist between two interacting proteins is computationally modelled by multi-label learning framework. The problem of GO (gene ontology) sparsity is tackled by introducing the homolog knowledge as independent homolog instances. ℓ2-regularized logistic regression is accordingly adopted here to penalize the homolog noise and to reduce the computational complexity of the double-sized training data. Computational results show that the proposed method achieves satisfactory multi-label learning performance and outperforms the existing phenotype correlation method on the experimental data of Drosophila melanogaster. Several predictions have been validated against recent literature. The predicted activation/inhibition relationships in human PPI networks are provided in the supplementary file for further biomedical research.
Novel CD200 homologues iSEC1 and iSEC2 are gastrointestinal secretory cell-specific ligands of inhibitory receptor CD200R
Authors: Kojima Toshiyuki , Tsuchiya Kiichiro , Ikemizu Shinji , Yoshikawa Soichiro , Yamanishi Yoshinori , Watanabe Mamoru , Karasuyama Hajime
Published: November 2016

CD200R is an inhibitory receptor expressed on myeloid cells and some lymphoid cells, and plays important roles in negatively regulating immune responses. CD200 is the only known ligand of CD200R and broadly distributed in a variety of cell types. Here we identified novel CD200 homologues, designated iSEC1 and iSEC2, that are expressed exclusively by secretory cell lineages in the gastrointestinal epithelium while authentic CD200 is expressed by none of epithelial cells including secretory cells. Both iSEC1 and iSEC2 could bind to CD200R but not other members of the CD200R family. Notably, CD200R expression was confined to intraepithelial lymphocytes (IELs) among cells in the gastrointestinal epithelium. Binding of iSEC1 to CD200R on IELs resulted in the suppression of cytokine production and cytolytic activity by activated IELs. Thus, iSEC1 is a previously unappreciated CD200R ligand with restricted expression in gastrointestinal secretory cells and may negatively regulate mucosal immune responses.
Big genomics and clinical data analytics strategies for precision cancer prognosis
Authors: Ow Ghim Siong , Kuznetsov Vladimir A.
Published: November 2016

The field of personalized and precise medicine in the era of big data analytics is growing rapidly. Previously, we proposed our model of patient classification termed Prognostic Signature Vector Matching (PSVM) and identified a 37 variable signature comprising 36 let-7b associated prognostic significant mRNAs and the age risk factor that stratified large high-grade serous ovarian cancer patient cohorts into three survival-significant risk groups. Here, we investigated the predictive performance of PSVM via optimization of the prognostic variable weights, which represent the relative importance of one prognostic variable over the others. In addition, we compared several multivariate prognostic models based on PSVM with classical machine learning techniques such as K-nearest-neighbor, support vector machine, random forest, neural networks and logistic regression. Our results revealed that negative log-rank p-values provides more robust weight values as opposed to the use of other quantities such as hazard ratios, fold change, or a combination of those factors. PSVM, together with the classical machine learning classifiers were combined in an ensemble (multi-test) voting system, which collectively provides a more precise and reproducible patient stratification. The use of the multi-test system approach, rather than the search for the ideal classification/prediction method, might help to address limitations of the individual classification algorithm in specific situation.
Micro-CT evaluation of apical delta morphologies in human teeth
Authors: Gao Xianhua , Tay Franklin R. , Gutmann James L. , Fan Wei , Xu Ting , Fan Bing
Published: November 2016

The apical delta is an intricate system within the root canal and incompletely debridement may affect the long-term prognosis of root canal therapy. The aim of the present study is to investigate the morphologic features of apical deltas in human teeth with micro-computed tomography (micro-CT) using a centreline-fitting algorithm. One hundred and thirty-six apical deltas were detected in 1400 teeth. Molars had more apical deltas (15.8%) than anterior teeth (6.3%). In maxillary molars, the mesiobuccal root had a significantly higher prevalence of apical delta than the palatal root or the distobuccal root. The median vertical distance of the apical delta was 1.87 mm with 13% more than 3 mm. The median diameter and length of the apical delta branches were 132.3 and 934.5 μm. Apical delta branches were not straight with cross-sectional shapes being non-circular. These morphological features of apical delta may complicate debridement of the infected root canal system.
Automatic detection and measurement of viral replication compartments by ellipse adjustment
Authors: Garcés Yasel , Guerrero Adán , Hidalgo Paloma , López Raul Eduardo , Wood Christopher D. , Gonzalez Ramón A. , Rendón-Mancha Juan Manuel
Published: November 2016

Viruses employ a variety of strategies to hijack cellular activities through the orchestrated recruitment of macromolecules to specific virus-induced cellular micro-environments. Adenoviruses (Ad) and other DNA viruses induce extensive reorganization of the cell nucleus and formation of nuclear Replication Compartments (RCs), where the viral genome is replicated and expressed. In this work an automatic algorithm designed for detection and segmentation of RCs using ellipses is presented. Unlike algorithms available in the literature, this approach is deterministic, automatic, and can adjust multiple RCs using ellipses. The proposed algorithm is non iterative, computationally efficient and is invariant to affine transformations. The method was validated over both synthetic images and more than 400 real images of Ad-infected cells at various timepoints of the viral replication cycle obtaining relevant information about the biogenesis of adenoviral RCs. As proof of concept the algorithm was then used to quantitatively compare RCs in cells infected with the adenovirus wild type or an adenovirus mutant that is null for expression of a viral protein that is known to affect activities associated with RCs that result in deficient viral progeny production.
Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow
Authors: Yumoto Kenji , Eber Matthew R. , Wang Jingcheng , Cackowski Frank C. , Decker Ann M. , Lee Eunsohl , Nobre Ana Rita , Aguirre-Ghiso Julio A. , Jung Younghun , Taichman Russell S.
Published: November 2016

Disseminated prostate cancer (PCa) cells in the marrow survive for years without evidence of proliferation, while maintaining the capacity to develop into metastatic lesions. These dormant disseminated tumor cells (DTCs) may reside in close proximity to osteoblasts, while expressing high levels of Axl, one of the tyrosine kinase receptors for growth arrest specific 6 (Gas6). Yet how Axl regulates DTC proliferation in marrow remains undefined. Here, we explored the impact of the loss of Axl in PCa cells (PC3 and DU145) on the induction of their dormancy when they are co-cultured with a pre-osteoblastic cell line, MC3T3-E1. MC3T3-E1 cells dramatically decrease the proliferation of PCa cells, however this suppressive effect of osteoblasts is significantly reduced by the reduction of Axl expression in PCa cells. Interestingly, expression of both TGF-β and its receptors were regulated by Axl expression in PCa cells, while specific blockade of TGF-β signaling limited the ability of the osteoblasts to induce dormancy of PCa cells. Finally, we found that both Gas6 and Axl are required for TGF-β2-mediated cell growth suppression. Taken together, these data suggest that a loop between the Gas6/Axl axis and TGF-β2 signaling plays a significant role in the induction of PCa cell dormancy.
Attosecond electronic and nuclear quantum photodynamics of ozone monitored with time and angle resolved photoelectron spectra
Authors: Decleva Piero , Quadri Nicola , Perveaux Aurelie , Lauvergnat David , Gatti Fabien , Lasorne Benjamin , Halász Gábor J. , Vibók Ágnes
Published: November 2016

Recently we reported a series of numerical simulations proving that it is possible in principle to create an electronic wave packet and subsequent electronic motion in a neutral molecule photoexcited by a UV pump pulse within a few femtoseconds. We considered the ozone molecule: for this system the electronic wave packet leads to a dissociation process. In the present work, we investigate more specifically the time-resolved photoelectron angular distribution of the ozone molecule that provides a much more detailed description of the evolution of the electronic wave packet. We thus show that this experimental technique should be able to give access to observing in real time the creation of an electronic wave packet in a neutral molecule and its impact on a chemical process.
PEPlife: A Repository of the Half-life of Peptides
Authors: Mathur Deepika , Prakash Satya , Anand Priya , Kaur Harpreet , Agrawal Piyush , Mehta Ayesha , Kumar Rajesh , Singh Sandeep , Raghava Gajendra P. S.
Published: November 2016

Short half-life is one of the key challenges in the field of therapeutic peptides. Various studies have reported enhancement in the stability of peptides using methods like chemical modifications, D-amino acid substitution, cyclization, replacement of labile aminos acids, etc. In order to study this scattered data, there is a pressing need for a repository dedicated to the half-life of peptides. To fill this lacuna, we have developed PEPlife (http://crdd.osdd.net/raghava/peplife), a manually curated resource of experimentally determined half-life of peptides. PEPlife contains 2229 entries covering 1193 unique peptides. Each entry provides detailed information of the peptide, like its name, sequence, half-life, modifications, the experimental assay for determining half-life, biological nature and activity of the peptide. We also maintain SMILES and structures of peptides. We have incorporated web-based modules to offer user-friendly data searching and browsing in the database. PEPlife integrates numerous tools to perform various types of analysis such as BLAST, Smith-Waterman algorithm, GGSEARCH, Jalview and MUSTANG. PEPlife would augment the understanding of different factors that affect the half-life of peptides like modifications, sequence, length, route of delivery of the peptide, etc. We anticipate that PEPlife will be useful for the researchers working in the area of peptide-based therapeutics.