bluuebunny/crossref_metadata_2025 huggingface dataset:
A systematic review and meta-analysis of the diagnosis and surgical management of carcinoid heart disease
Authors: Namkoong Jenny , Andraweera Prabha H. , Pathirana Maleesa , Munawar Dian , Downie Michael , Edwards Suzanne , Averbuj Paula , Arstall Margaret A.
Published: March 2024

Introduction

Carcinoid heart disease (CHD), a complication of carcinoid syndrome (CS), is a rare condition that can lead to right sided valvular heart disease and has been traditionally associated with a poor prognosis. We conducted a systematic review and meta-analysis to explore the accuracy of biomarkers and echocardiography in diagnosing CHD amongst patients who are already known to have neuroendocrine tumours and to assess whether surgical management of CHD leads to a reduction in mortality.

Methods

A systematic literature search of MEDLINE, EMBASE, EBM Reviews, Google Scholar, ClinicalTrials.gov was conducted. All studies on patients with carcinoid heart disease (CHD) reporting on biomarkers, echocardiographic and surgical outcomes were included. The National Heart, Lung, and Blood Institute quality assessment tool was used to assess the methodological study quality. Data analysis was performed using Stata Statistical Software and R Studio, and individual meta-analyses were performed for biomarkers, echocardiographic findings, and surgical outcomes.

Results

A total of 36 articles were included in the systematic review analysis. N terminal pro-brain natriuretic peptide (NTproBNP) and 5-hydroxyindole acetate (5-HIAA) levels were higher in patients with CHD compared with those without CHD. 32% of CS patients had echocardiographic evidence of cardiac involvement, of which 79% involved tricuspid valve abnormalities. Moderate-severe tricuspid regurgitation was the most common echocardiographic abnormality (70% of patients). However, these analyses had substantial heterogeneity due to the high variability of cardiac involvement across studies. Pooled surgical mortality for CHD was 11% at 1 month, 31% at 12 months and 56% at 24 months. When assessing surgical outcomes longitudinally, the one-month surgical results showed a trend towards more recent surgeries having lower mortality rates than those reported in earlier years, however this was not statistically significant.

Discussion

There is not enough data in current literature to determine a clear cut-off value of NTproBNP and 5-HIAA to help diagnose or determine CHD severity. Surgical management of CHD is yet to show significant mortality benefit, and there are no consistent comparisons to medical treatment in current literature.
The upper crustal thermal structure of the Cameli Basin and its surroundings (SW Anatolia, Türkiye) by using the fractal-based centroid method of aeromagnetic data and its relationship with earthquakes occurring in the region
Authors: Akar Fahriye
Published: March 2024

The Cameli Basin and its surroundings are located in southwestern Anatolia, Türkiye, and are one of the tectonically active regions in western Türkiye. The Curie point depth of continental crust can help us to determine the regional tectonic and geothermal structures. The aeromagnetic magnetic data of the study region were used to determine the Curie Point Depth estimates. The fractal-based centroid method is used for this purpose. The fractal approach removes the effect of fractal magnetization in the power spectrum. The depth to the bottom of the deepest magnetic sources in the study region ranges between 6.9 and 14.05. The estimated thermal gradient varies from 41.28°C/km to 89.23°C/km and the average value is 58.59°C/km (580°C for magnetite). The distributions of earthquakes are compatible with NW-SE trending estimated Curie depth anomalies. Interpretation indicates that the depth to the Curie isotherm is considerably shallower than the Moho depth.
Abnormal line loss identification and category classification of distribution networks based on semi-supervised learning and hierarchical classification
Authors: Li Wei , Zhao Wen , Li Junmin , Li Jie , Zhao Yankai
Published: March 2024

Line loss refers to the electrical energy that is dissipated as heat during the transmission and distribution of electricity through power lines. However, unusual causes, such as grid topology mismatch and communication failure, can cause abnormal line loss. Efficient abnormal line loss detection contributes not only to minimizing energy wastage and reducing carbon emissions but also to maintaining the stability and reliability of the entire distribution network. In actual situations, the cause of abnormal line loss is not labeled due to the expensive labor cost. This paper proposes a hierarchical abnormal line loss identification and category classification model, considering the unlabeled and unbalanced sample problem. First, an abnormal line loss identification model-based random forest is established to detect whether the line loss is abnormal. Then, an abnormal line loss category classification model is developed with semi-supervised learning for line loss abnormal category classification, considering the unlabeled samples. The real dataset in China is utilized to validate the performance of the proposed model. Its reliability implies the potential to be applied to real-world scenarios to improve the management level and safety of the power grid.
Ultrasound-triggered with ROS-responsive SN38 nanoparticle for enhanced combination cancer immunotherapy
Authors: Liu Hongyu , Shi Yunpeng , Ji Guofeng , Wang Jukun , Gai Baodong
Published: March 2024

Controlled generation of cytotoxic reactive oxygen species (ROS) is essential in cancer therapy. Ultrasound (US)-triggered sonodynamic therapy (SDT) has shown considerable ability to trigger in situ ROS generation. Unfortunately, US therapy alone is insufficient to trigger an efficient anticancer response, owing to the induction of multiple immunosuppressive factors. It was identified that 7-ethyl-10-hydroxycamptothecin (SN38) could notably inhibit DNA topoisomerase I, induce DNA damage and boost robust anticancer immunity. However, limited by the low metabolic stability, poor bioavailability, and dose-limiting toxicity, the direct usage of SN38 is inadequate in immune motivation, which limits its clinical application. Hence, new strategies are needed to improve drug delivery efficiency to enhance DNA topoisomerase I inhibition and DNA damage and elicit a vigorous anticancer cancer immunity response. Considering US irradiation can efficiently generate large amounts of ROS under low-intensity irradiation, in this study, we aimed to design a polymeric, ROS-responsive SN38 nanoformulation for in vivo drug delivery. Upon the in-situ generation of ROS by US therapy, controlled on-demand release of SN38 occurred in tumor sites, which enhanced DNA damage, induced DC cell maturation, and boosted anticancer immunity. Our results demonstrated that a new strategy of involving the combination of a SN38 nanoformulation and US therapy could be used for cancer immunotherapy.
The ST2+ Treg/amphiregulin axis protects from immune-mediated hepatitis
Authors: Wachtendorf Selina , Jonin Fitriasari , Ochel Aaron , Heinrich Fabian , Westendorf Astrid M. , Tiegs Gisa , Neumann Katrin
Published: March 2024

Introduction

The alarmin IL-33 has been implicated in the pathology of immune-mediated liver diseases. IL-33 activates regulatory T cells (Tregs) and type 2 innate lymphoid cells (ILC2s) expressing the IL-33 receptor ST2. We have previously shown that endogenous IL-33/ST2 signaling activates ILC2s that aggravate liver injury in murine immune-mediated hepatitis. However, treatment of mice with exogenous IL-33 before induction of hepatitis ameliorated disease severity. Since IL-33 induces expression of amphiregulin (AREG) crucial for Treg function, we investigated the immunoregulatory role of the ST2+ Treg/AREG axis in immune-mediated hepatitis.

Methods

C57BL/6, ST2-deficient (Il1rl1-/-) and Areg-/- mice received concanavalin A to induce immune-mediated hepatitis. Foxp3Cre+ x ST2fl/fl mice were pre-treated with IL-33 before induction of immune-mediated hepatitis. Treg function was assessed by adoptive transfer experiments and suppression assays. The effects of AREG and IL-33 on ST2+ Tregs and ILC2s were investigated in vitro. Immune cell phenotype was analyzed by flow cytometry.

Results and discussion

We identified IL-33-responsive ST2+ Tregs as an effector Treg subset in the murine liver, which was highly activated in immune-mediated hepatitis. Lack of endogenous IL-33 signaling in Il1rl1-/- mice aggravated disease pathology. This was associated with reduced Treg activation. Adoptive transfer of exogenous IL-33-activated ST2+ Tregs before induction of hepatitis suppressed inflammatory T-cell responses and ameliorated disease pathology. We further showed increased expression of AREG by hepatic ST2+ Tregs and ILC2s in immune-mediated hepatitis. Areg-/- mice developed more severe liver injury, which was associated with enhanced ILC2 activation and less ST2+ Tregs in the inflamed liver. Exogenous AREG suppressed ILC2 cytokine expression and enhanced ST2+ Treg activation in vitro. In addition, Tregs from Areg-/- mice were impaired in their capacity to suppress CD4+ T-cell activation in vitro. Moreover, application of exogenous IL-33 before disease induction did not protect Foxp3Cre+ x ST2fl/fl mice lacking ST2+ Tregs from immune-mediated hepatitis. In summary, we describe an immunoregulatory role of the ST2+ Treg/AREG axis in immune-mediated hepatitis, in which AREG suppresses the activation of hepatic ILC2s while maintaining ST2+ Tregs and reinforcing their immunosuppressive capacity in liver inflammation.
Neutralizing antibody and CD8+ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2
Authors: Underwood Alexander P. , Sølund Christina , Jacobsen Kivin , Binderup Alekxander , Fernandez-Antunez Carlota , Mikkelsen Lotte S. , Inekci Dilek , Villadsen Signe Lysemose , Castruita Jose A. S. , Pinholt Mette , Fahnøe Ulrik , Ramirez Santseharay , Brix Liselotte , Weis Nina , Bukh Jens
Published: March 2024

As severe acute respiratory coronavirus 2 (SARS-CoV-2) variants continue to emerge, it is important to characterize immune responses against variants which can inform on protection efficacies following booster vaccination. In this study, neutralizing breadth and antigen-specific CD8+ T cell responses were analyzed in both infection-naïve and infection-experienced individuals following administration of a booster bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine. Significantly higher neutralizing titers were found after this vaccination compared to the pre-third booster vaccination time point. Further, neutralizing breadth to omicron variants, including BA.1, BA.2, BA.5, BQ.1 and XBB.1, was found to be boosted following bivalent vaccination. SARS-CoV-2-specific CD8+ T cells were identified, but with no evidence that frequencies were increased following booster vaccinations. Spike protein-specific CD8+ T cells were the only responses detected after vaccination and non-spike-specific CD8+ T cells were only detected after infection. Both spike-specific and non-spike-specific CD8+ T cells were found at much lower frequencies than CD8+ T cells specific to cytomegalovirus (CMV), Epstein-Barr virus (EBV) and influenza (Flu). Taken together, these results show that the bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine boosted the breadth of neutralization to newer SARS-CoV-2 variants and that vaccination is able to induce spike protein-specific CD8+ T cell responses, which are maintained longitudinally.
How do sea urchins prepare offspring to face ocean acidification? Gamete intraspecific differences and adaptability
Authors: Asnicar Davide , Locatello Lisa , Zanovello Laura , Minichino Riccardo , Masiero Luciano , Munari Marco , Marin Maria Gabriella
Published: March 2024

Introduction

Due to their relevant ecological position and well-studied biology, sea urchins are reference organisms for ocean acidification studies, at both within- and trans-generational levels. In this study, we examined gamete quality in specimens exposed to future predicted (-0.4 units) pH conditions during gametogenesis.

Methods

Egg physical characteristics, biochemical composition, and fatty acid profiles were assessed after two and six months of exposure, while sperm viability and velocity were analyzed after six months of exposure. Considering the documented intraspecific variability in response to ocean acidification, this study involved two populations of Paracentrotus lividus. One population was sampled from the highly variable lagoon of Venice (Site 1), while the other was obtained from a coastal area (Site 2) characterized by more stable environmental conditions and facing minimal anthropogenic stress.

Results

A different response was highlighted in the two sites. Noteworthy trends emerged, especially in the fatty acid profile and sperm traits. Although adults were fed the same diet, Site 1 eggs contained more high-energetic fatty acids than Site 2, potentially boosting the survival odds for the next generation. Moreover, Site 1 sperms displayed higher viability but slower motility compared to those from Site 2. Within sites, a significant difference between time points and a change in the fitness strategy of sea urchin females emerged when comparing eggs spawned after two and six months of exposure to reduced pH. The effects of time and exposure pH are more pronounced in animals from Site 1, suggesting a higher adaptability of this population rather than negative effects of ocean acidification.

Discussion

Overall, our findings suggest that sea urchins have the potential to acclimate to reduced pH and to produce gametes of the same quality as controls held at the currently natural pH. Our findings emphasize the relevance of combining investigations of gamete quality characteristics, particularly egg biochemistry and fatty acid composition, and considering site variability to fully understand the transgenerational response potential of sea urchins to ocean acidification.
18F-FDG PET/CT revealed sporadic schwannomatosis involving the lumbar spinal canal and both lower limbs: a case report
Authors: Li Xiaotian , Hu Xianwen , Wang Pan , Cai Jiong
Published: March 2024

Schwannomatosis is a rare autosomal dominant hereditary syndrome disease characterized by multiple schwannomas throughout the body, without bilateral vestibular schwannoma or dermal schwannoma. The most common location of schwannomatosis is the head and neck, as well as the limbs, while multiple schwannomas in the lumbosacral canal and lower extremities are relatively rare. In this study, we report a 79-year-old woman diagnosed with schwannomatosis. MRI and contrast-enhanced imaging revealed multiple schwannomas in both lower extremities. An 18F-FDG PET/CT examination revealed that in addition to multiple tumors with increased 18F-FDG uptake in both lower extremities, there was also an increased 18F-FDG uptake in a mass in the lumbosacral canal. These masses were confirmed to be schwannomas by pathology after surgery or biopsy. 18F-FDG PET/CT findings of schwannomas were correlated with MRI and pathological components. Antoni A area rich in tumor cells showed significant enhancement on contrast-enhanced T1WI, and PET/CT showed increased uptake of 18F-FDG in the corresponding area, while Antoni B region rich in mucus showed low enhancement on contrast-enhanced T1WI, accompanied by a mildly increased 18F-FDG uptake.
Overexpression of the FBA and TPI genes promotes high production of HDMF in Zygosaccharomyces rouxii
Authors: Wang Yanhong , Liu Wei , Chen Jingyao , Li Zhijiang , Hu Yijia , Fan Zixiang , Yan Liangyuan , Liu Jiahui , Zhou Yuao , Jiang Wei , Rui Haiying , Dai Lingyan
Published: March 2024

4-Hydroxy-2,5-dimethyl-3 (2H)-furanone (HDMF) is widely used in the food industry as a spice and flavoring agent with high market demand. In this study, fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) were overexpressed in Zygosaccharomyces rouxii in the form of single and double genes, respectively, via electroporation. High-yield HDMF-engineered yeast strains were constructed by combining the analysis of gene expression levels obtained by real-time fluorescence quantitative PCR technology and HDMF production measured by HPLC. The results showed that there was a significant positive correlation between the production of HDMF and the expression levels of the FBA and TPI genes in yeast; the expression levels of the FBA and TPI genes were also positively correlated (p < 0.05). Compared with the wild type (WT), the engineered strains F10-D, T17-D, and TF15-A showed marked increases in HDMF production and FBA and TPI gene expression (p < 0.05) and exhibited great genetic stability with no obvious differences in biomass or colony morphology. In addition, the exogenous addition of d-fructose promoted the growth of Z. rouxii. Among the engineered strains, when fermented in YPD media supplemented with d-fructose for 5 days, TF15-A (overexpressing the FBA and TPI genes) generated the highest HDMF production of 13.39 mg/L, which is 1.91 times greater than that of the wild-type strain. The results above indicated that FBA and TPI, which are key enzymes involved in the process of HDMF biosynthesis by Z. rouxii, positively regulate the synthesis of HDMF at the transcriptional level. d-fructose can be used as a precursor for the biosynthesis of HDMF by engineered yeast in industrial production.
Study on the effect and mechanism of Lacticaseibacillus rhamnosus AFY06 on inflammation-associated colorectal cancer induced by AOM/DSS in mice
Authors: Zhang Jing , Zhang Piyun , Li Sijia , Yu Ting , Lai Xiangyu , He Yongpeng
Published: March 2024

Introduction

Lacticaseibacillus rhamnosus AFY06 (LR-AFY06) is a microorganism isolated from naturally fermented yogurt in Xinjiang, China.

Methods

In this study, we investigated the effects and mechanisms of LR-AFY06 in a mouse model of inflammation-associated colon cancer. The mouse model was established by azoxymethane/dextran sulfate sodium (AOM/DSS) induction. The tumor number in intestinal tissues was counted, and the histopathological analysis was performed on colon tissues. Enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction were performed to measure relevant protein levels in colon tissues.

Results

LR-AFY06 treatment alleviated weight loss, increased organ index, reduced intestinal tumor incidence, improved histopathological damage, decreased the levels of inflammatory cytokines such as interleukin-6 (IL-6), interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), nuclear factor κB (NF-κB), and inducible nitric oxide synthase (iNOS) in the serum and colon tissue, downregulated the mRNA expression of inhibitor of NF-κB beta (IκBβ), p65, p50, p52, B-cell lymphoma-2 (Bcl-2), and B-cell lymphoma-extra large (Bcl-xL) in colon tissues, and increased the mRNA expression of Bid and caspase-8. The high concentration of LR-AFY06 exerted a better effect than the low concentration; however, the effect was slightly inferior to that of aspirin. Moreover, LR-AFY06 mitigated the intestinal inflammatory process and inhibited intestinal tumor development by regulating the NF-κB and apoptosis pathways.

Discussion

The present study indicates the regulatory potential of LR-AFY06 in inflammation-associated colorectal cancer in mice, providing a valuable basis for further research.
The association of TNF-alpha secretion and mtDNA copy number in CD14+ monocytes of patients with obesity and CHD
Authors: Tolstik Taisiya V. , Kirichenko Tatiana V. , Markin Alexander M. , Bogatyreva Anastasia I. , Markina Yuliya V. , Kiseleva Diana G. , Shaposhnikova Nataliya N. , Starodubova Antonina V. , Orekhov Alexander N.
Published: March 2024

Introduction

Mitochondrial dysfunction may be one of the causes of inflammatory activation of monocytes and macrophages, which leads to excessive secretion of inflammatory mediators and the development of chronic inflammation.

Aims

The study was aimed to evaluate the secretion of inflammatory cytokine tumor necrosis factor-α (TNF-α) in the primary culture of monocytes, and to analyze its relationship with the number of mitochondrial DNA (mtDNA) copies in the blood of patients with coronary heart disease (CHD) and obesity.

Materials and methods

108 patients with obesity and concomitant CHD and a control group of 25 participants were included in the study. CD14+ monocytes were isolated by a standard method in a ficoll-urographin gradient, followed by separation using magnetic particles. The number of mtDNA copies was estimated using qPCR.

Results

It was demonstrated that the number of mtDNA copies was significantly increased in groups of patients with CHD and obesity + CHD in comparison with control group. mtDNA copy number positively correlated with basal and LPS-stimulated TNF-α secretion, the most significant correlation was found in the group of patients with CHD and obesity.

Conclusion

Thus, the change in mtDNA copy number in CD14+ monocytes which indicates the presence of mitochondrial dysfunction, confirm the direct involvement of mitochondria in the violation of the inflammatory response of monocytes revealed in this study as an increased secretion of inflammatory cytokine TNF-α.
Prevalence and risk factors of early postoperative seizures in patients with glioma: a systematic review and meta-analysis
Authors: Sun Bo , Sun Yuchen , Wang Zijian , Zhao Chao , Yang Liang
Published: March 2024

Objective

This study aimed to explore the prevalence and risk factors of early postoperative seizures in patients with glioma through meta-analysis.

Methods

Case–control studies and cohort studies on the prevalence and risk factors of early postoperative seizures in glioma patients were retrieved from various databases including CNKI, Wanfang, VIP, PubMed, Embase, Cochrane Library, and Web of Science, and the retrieval deadline for the data was 1 April 2023. Stata15.0 was used to analyze the data.

Results

This review included 11 studies consisting of 488 patients with early postoperative seizures and 2,051 patients without early postoperative seizures. The research findings suggest that the prevalence of glioma is complicated by seizures (ES = 19%, 95% confidence interval [CI] [14%−25%]). The results also indicated a history of seizures (RR = 1.94, 95% CI [1.76, 2.14], P = 0.001), preoperative dyskinesia (RR = 3.13, 95% CI [1.20, 8.15], P = 0.02), frontal lobe tumor (RR = 1.45, 95% CI [1.16, 1.83], P = 0.001), pathological grade ≤2 (RR = 1.74, 95% CI [1.13, 2.67], P = 0.012), tumor≥ 3 cm (RR = 1.70, 95% CI [1.18, 2.45], P = 0.005), tumor resection (RR = 1.60, 95% CI [1.36, 1.88], P = 0.001), tumor edema ≥ 2 cm (RR = 1.77, 95% CI [1.40, 2.25], P = 0.001), and glioma cavity hemorrhage (RR=3.15, 95% CI [1.85, 5.37], P = 0.001). The multivariate analysis results showed that a history of seizures, dyskinesia, tumor ≥3 cm, peritumoral edema ≥2 cm, and glioma cavity hemorrhage were indicated as risk factors for glioma complicated with early postoperative seizures.

Significance

Based on the existing evidence, seizure history, dyskinesia, frontal lobe tumor, pathological grade ≤2, tumor ≥3 cm, partial tumor resection, edema around tumor ≥2 cm, and glioma cavity hemorrhage are indicated as risk factors for glioma complicated with early postoperative seizures.
Trichostatin A relieves anxiety-and depression-like symptoms in APP/PS1 mice
Authors: Su Qiang , Ren Yu-Hua , Liu Guo-Wei , Gao Yan-Ping , Zhang Jiu-Xuan , Zhang Jin-Nan , Pei Xia-Xia , Li Tian
Published: March 2024

Background:

Cognitive deficits and behavioral disorders such as anxiety and depression are common manifestations of Alzheimer’s disease (AD). Our previous work demonstrated that Trichostatin A (TSA) could alleviate neuroinflammatory plaques and improve cognitive disorders. AD, anxiety, and depression are all associated with microglial inflammation. However, whether TSA could attenuate anxiety- and depression-like behaviors in APP/PS1 mice through anti-inflammatory signaling is still unclearly.

Methods:

In the present study, all mice were subjected to the open field, elevated plus maze, and forced swim tests to assess anxiety- and depression-related behaviors after TSA administration. To understand the possible mechanisms underlying the behavioral effects observed, CST7 was measured in the hippocampus of mice and LPS-treated BV2 microglia.

Results:

The results of this study indicated that TSA administration relieved the behaviors of depression and anxiety in APP/PS1 mice, and decreased CST7 levels in the hippocampus of APP/PS1 mice and LPS-induced BV2 cells.

Conclusion:

Overall, these findings support the idea that TSA might be beneficial for reducing neurobehavioral disorders in AD and this could be due to suppression of CST7-related microglial inflammation.
Overexpression of SLIM1 transcription factor accelerates vegetative development in Arabidopsis thaliana
Authors: Apodiakou Anastasia , Alseekh Saleh , Hoefgen Rainer , Whitcomb Sarah J.
Published: March 2024

The transcription factor Sulfur Limitation 1 (SLIM1) belongs to the plant-specific Ethylene Insenstive3-Like transcription factor family and is known to coordinate gene expression in response to sulfur deficiency. However, the roles of SLIM1 in nutrient-sufficient conditions have not been characterized. Employing constitutive SLIM1 overexpression (35S::SLIM1) and CRISPR/Cas9 mutant plants (slim1-cr), we identified several distinct phenotypes in nutrient-sufficient conditions in Arabidopsis thaliana. Overexpression of SLIM1 results in plants with approximately twofold greater rosette area throughout vegetative development. 35S::SLIM1 plants also bolt earlier and exhibit earlier downregulation of photosynthesis-associated genes and earlier upregulation of senescence-associated genes than Col-0 and slim1-cr plants. This suggests that overexpression of SLIM1 accelerates development in A. thaliana. Genome-wide differential gene expression analysis relative to Col-0 at three time points with slim1-cr and two 35S::SLIM1 lines allowed us to identify 1,731 genes regulated directly or indirectly by SLIM1 in vivo.
Exploring the potential of endophyte-plant interactions for improving crop sustainable yields in a changing climate
Authors: Sena Lorenzo , Mica Erica , Valè Giampiero , Vaccino Patrizia , Pecchioni Nicola
Published: March 2024

Climate change poses a major threat to global food security, significantly reducing crop yields as cause of abiotic stresses, and for boosting the spread of new and old pathogens and pests. Sustainable crop management as a route to mitigation poses the challenge of recruiting an array of solutions and tools for the new aims. Among these, the deployment of positive interactions between the micro-biotic components of agroecosystems and plants can play a highly significant role, as part of the agro-ecological revolution. Endophytic microorganisms have emerged as a promising solution to tackle this challenge. Among these, Arbuscular Mycorrhizal Fungi (AMF) and endophytic bacteria and fungi have demonstrated their potential to alleviate abiotic stresses such as drought and heat stress, as well as the impacts of biotic stresses. They can enhance crop yields in a sustainable way also by other mechanisms, such as improving the nutrient uptake, or by direct effects on plant physiology. In this review we summarize and update on the main types of endophytes, we highlight several studies that demonstrate their efficacy in improving sustainable yields and explore possible avenues for implementing crop-microbiota interactions. The mechanisms underlying these interactions are highly complex and require a comprehensive understanding. For this reason, omic technologies such as genomics, transcriptomics, proteomics, and metabolomics have been employed to unravel, by a higher level of information, the complex network of interactions between plants and microorganisms. Therefore, we also discuss the various omic approaches and techniques that have been used so far to study plant-endophyte interactions.
A new model construction based on the knowledge graph for mining elite polyphenotype genes in crops
Authors: Zhang Dandan , Zhao Ruixue , Xian Guojian , Kou Yuantao , Ma Weilu
Published: March 2024

Identifying polyphenotype genes that simultaneously regulate important agronomic traits (e.g., plant height, yield, and disease resistance) is critical for developing novel high-quality crop varieties. Predicting the associations between genes and traits requires the organization and analysis of multi-dimensional scientific data. The existing methods for establishing the relationships between genomic data and phenotypic data can only elucidate the associations between genes and individual traits. However, there are relatively few methods for detecting elite polyphenotype genes. In this study, a knowledge graph for traits regulating-genes was constructed by collecting data from the PubMed database and eight other databases related to the staple food crops rice, maize, and wheat as well as the model plant Arabidopsis thaliana. On the basis of the knowledge graph, a model for predicting traits regulating-genes was constructed by combining the data attributes of the gene nodes and the topological relationship attributes of the gene nodes. Additionally, a scoring method for predicting the genes regulating specific traits was developed to screen for elite polyphenotype genes. A total of 125,591 nodes and 547,224 semantic relationships were included in the knowledge graph. The accuracy of the knowledge graph-based model for predicting traits regulating-genes was 0.89, the precision rate was 0.91, the recall rate was 0.96, and the F1 value was 0.94. Moreover, 4,447 polyphenotype genes for 31 trait combinations were identified, among which the rice polyphenotype gene IPA1 and the A. thaliana polyphenotype gene CUC2 were verified via a literature search. Furthermore, the wheat gene TraesCS5A02G275900 was revealed as a potential polyphenotype gene that will need to be further characterized. Meanwhile, the result of venn diagram analysis between the polyphenotype gene datasets (consists of genes that are predicted by our model) and the transcriptome gene datasets (consists of genes that were differential expression in response to disease, drought or salt) showed approximately 70% and 54% polyphenotype genes were identified in the transcriptome datasets of Arabidopsis and rice, respectively. The application of the model driven by knowledge graph for predicting traits regulating-genes represents a novel method for detecting elite polyphenotype genes.
How much heat does non-photochemical quenching produce?
Authors: Murakami Aoi , Kim Eunchul , Minagawa Jun , Takizawa Kenji
Published: March 2024

Non-photochemical quenching (NPQ) is a protective mechanism used by plants to safely dissipate excess absorbed light energy as heat, minimizing photo-oxidative damage. Although the importance of NPQ as a safety valve for photosynthesis is well-known, the physiological and environmental effects of the heat produced remain unclear because the amount of heat produced by NPQ is considered negligible, and its physiological effects have not been directly observed. Here, we calculated the heat produced by NPQ and evaluated its impact on the leaf and global warming based on simplified models. Our evaluation showed that the heat produced by NPQ in a given leaf area is 63.9 W m−2 under direct sunlight. Under the standard condition, NPQ warms up the leaf at less than 0.1°C, but it could be 1°C under particular conditions with low thermal conductance. We also estimated the thermal radiation of vegetation’s NPQ to be 2.2 W m−2 par global averaged surface area. It is only 0.55% of the thermal radiation by the Earth’s surface, but still significant in the current climate change response. We further discuss the possible function of NPQ to plant physiology besides the safety valve and provide strategies with artificial modification of the NPQ mechanism to increase food production and mitigate global warming.
Collective action against corruption in Western and non-Western countries: cross-cultural implications of the Axiological-Identitary Collective Action Model
Authors: Grigoryev Dmitry , Gallyamova Albina , Conway Lucian Gideon , Zubrod Alivia , Sabucedo José Manuel , Dono Marcos , Batkhina Anastasia , Boehnke Klaus
Published: March 2024

People sometimes protest government corruption, yet our current understanding of why they do so is culturally constrained. Can we separate pancultural factors influencing people’s willingness to protest government corruption from factors culturally specific to each socioecological context? Surprisingly little cross-cultural data exist on this important question. To fill this gap, we performed a cross-cultural test of the Axiological-Identitary Collective Action Model (AICAM) regarding the intention to protest against corruption. As a collective action framework, AICAM integrates three classical antecedents of collective action (injustice, efficacy, identity) with axiological variables (ideology and morality). A total sample of 2,316 participants from six countries (Nigeria, Russia, India, Spain, United States, Germany) in a multilevel analysis of AICAM predictions showed that the positive relationship of the intention to protest corruption with moral obligation, system-based anger, and national identification can be considered pancultural. In contrast, the relationships between system justification and perceived efficacy are culturally specific. System justification negatively predicted the intention to participate only in countries with high levels of wealth, while perceived efficacy positively predicted it only in countries perceived as less corrupt. These findings highlight the importance of accounting features of socioecology and separating pancultural from culture-specific effects in understanding collective action.
The effects of live streamer’s expertise and entertainment on the viewers’ purchase and follow intentions
Authors: Jiang Yaping , Lee Hyoung-Tark , Li Wei
Published: March 2024

This study explores the impact of two characteristics of streamers—expertise and entertainment—on viewers’ purchase intention and follow intention in live-streaming e-commerce, with a specific focus on viewers’ trust and flow experience as two mediators and viewers’ optimal stimulation level as a moderator. We implemented a methodological approach where participants were randomly directed to enter a live broadcast room and watch a 10-min live session before engaging in a structured questionnaire. 399 valid questionnaires were collected from the participants. These 399 valid questionnaires were subsequently utilized to validate the research model using structural equation modeling (SEM). The results suggest that streamer expertise and entertainment enhance viewers’ trust and flow experience, which then leads to an increase in their intention to make a purchase and continue following the streamer. Furthermore, the viewers’ optimal stimulation level acts as a moderator in the connections between streamer characteristics and viewers’ trust and flow experience, suggesting that individual differences among consumers affect how they respond to streamer characteristics. From the dual perspectives of the streamer and the viewer, this study provides a more comprehensive theoretical perspective on customer behavior in live streaming commerce by not only focusing on consumers’ short-term, transactional behavior inclinations but also long-term, relational behavior intentions.
Principles to guide research and policy on psychological well-being in remote island developing states in the South Pacific
Authors: Orbán Levente L.
Published: March 2024

Adverse climatic changes around the globe and predictions of catastrophic and irreversible alteration in global weather patterns, temperature rise, and coast-line habitability require a careful examination of consequences on the resilience and mental health of people who will endure these changes. This paper is concerned with the South Pacific region. This geography has benefited from a relatively stable climate that is seen in the lush and vibrant natural world with many unique species of plants and animals exclusively found here. This paper examines the psychological profile of the people in the South Pacific using an evolutionary framework, and considers their local climate risks and lifestyle patterns with the aim of exploring possible mental health trajectories.
Impact of social support on the resilience of youth: mediating effects of coping styles
Authors: Cao Fei , Li Juan , Xin Wei , Cai Nan
Published: March 2024

Background

Chinese youth are at high risk for depression with a significantly higher detection rate of depression risk than other age groups, which brings about a huge challenge to the mental health work of universities. Developing supportive resources that promote resilience against adverse environmental influences in high-risk groups is quite more urgent than medical treatment for firm diagnoses of mental issues that have developed into depression in the current background.

Methods

A total of 665 university students in China completed self-reported questionnaires measuring psychological resilience, social support, and coping styles. The structural equation model testing on the goodness of fit of the theoretical framework was first performed. Descriptive statistics and Pearson’s correlation analysis among social support, resilience, and coping styles were then conducted. At last, we tested the mediating role of coping styles.

Results

Social support has a significant positive effect on the psychological resilience of the youth. Mixed coping and immature coping styles have significant negative impacts on both social support and resilience, while mature coping styles have a significant positive effect on social support and resilience. Mature and immature coping styles mediate the association between social support and resilience in youth.

Conclusion

Based on stress theory, this study explores mechanisms that facilitate the development of resilience in young people with regard to social support and coping styles. The current research depicts an interventional perspective of building a social support network that guides the youth to adopt mature coping styles to enhance their resilience and facilitate their mental health.
Anaesthesiologic Considerations for Intraoperative ECMO Anticoagulation During Lung Transplantation: A Single-Centre, Retrospective, Observational Study
Authors: Vajter Jaromir , Holubova Gabriela , Novysedlak Rene , Svorcova Monika , Vachtenheim Jiri , Vymazal Tomas , Lischke Robert
Published: March 2024

Background: Extracorporeal membrane oxygenation (ECMO) is frequently used during lung transplantation. Unfractionated heparin (UFH) is mainly used as part of ECMO support for anticoagulation. One of the most common perioperative complications is bleeding, which high-dose UFH can aggravate.

Methods: We retrospectively analyzed (n = 141) patients who underwent lung transplantation between 2020 and 2022. All subjects (n = 109) underwent central cannulated VA ECMO with successful intraoperative ECMO weaning. Patients on ECMO bridge, postoperative ECMO, heart-lung transplants and transplants without ECMO were excluded. The dose of UFH for the entire surgical procedure, blood loss and consumption of blood derivatives intraoperatively and 48 h after ICU admission were recorded. Surgical revision for postoperative bleeding were analyzed. Thrombotic complications, mortality and long-term survival were evaluated.

Results: Lower doses of UFH administered for intraoperative ECMO anticoagulation contribute to a reduction in intraoperative blood derivates consumption and blood loss with no thrombotic complications related to the patient or the ECMO circuit. Lower doses of UFH may lead to a decreased incidence of surgical revision for hemothorax.

Conclusion: Lower doses of UFH as part of intraoperative ECMO anticoagulation might reduce the incidence of complications and lead to better postoperative outcomes.
On the Need for Accurate Brushstroke Segmentation of Tablet-Acquired Kinematic and Pressure Data: The Case of Unconstrained Tracing
Authors: Franz Karly S. , Reszetnik Grace , Chau Tom
Published: March 2024

Brushstroke segmentation algorithms are critical in computer-based analysis of fine motor control via handwriting, drawing, or tracing tasks. Current segmentation approaches typically rely only on one type of feature, either spatial, temporal, kinematic, or pressure. We introduce a segmentation algorithm that leverages both spatiotemporal and pressure features to accurately identify brushstrokes during a tracing task. The algorithm was tested on both a clinical and validation dataset. Using validation trials with incorrectly identified brushstrokes, we evaluated the impact of segmentation errors on commonly derived biomechanical features used in the literature to detect graphomotor pathologies. The algorithm exhibited robust performance on validation and clinical datasets, effectively identifying brushstrokes while simultaneously eliminating spurious, noisy data. Spatial and temporal features were most affected by incorrect segmentation, particularly those related to the distance between brushstrokes and in-air time, which experienced propagated errors of 99% and 95%, respectively. In contrast, kinematic features, such as velocity and acceleration, were minimally affected, with propagated errors between 0 to 12%. The proposed algorithm may help improve brushstroke segmentation in future studies of handwriting, drawing, or tracing tasks. Spatial and temporal features derived from tablet-acquired data should be considered with caution, given their sensitivity to segmentation errors and instrumentation characteristics.
Trends of the Global Burden of Disease Linked to Ground-Level Ozone Pollution: A 30-Year Analysis for the Greater Athens Area, Greece
Authors: Ntourou Kleopatra , Fameli Kyriaki-Maria , Moustris Konstantinos , Manousakis Nikolaos , Tsitsis Christos
Published: March 2024

The Greater Athens Area (GAA), situated in the southern part of the European continent (in Greece), has a Mediterranean climate characterized by hot, dry summers and mild, wet winters. As a result of increased sunshine and high temperatures, exceedances in ozone concentrations are often recorded during the hot period. In the present study, the monthly as well as daily variations of O3 concentrations at thirteen stations in the GAA were investigated for the period 1987–2019. Moreover, the impact of O3 on the people’s health in Greece was examined by using data from the Global Burden of Disease (GBD) study with the socio-economic conditions of the country. Ozone concentrations were found to be particularly high during the summer months, especially in suburban stations. Values ranged from 65 μg/m3 to 90 μg/m3 during the night, in contrast to urban areas and remain high for several hours. Comparing estimates from GBD, it was found that exposure to ozone can impair respiratory function, leading to death or susceptibility to respiratory diseases that reduce quality of life, especially for people over 55 years of age. Finally, since 2009, when the economic crisis began in Greece, an upward trend was observed for deaths and disability adjusted life years.
Comparison of Coupled Model Intercomparison Project Phases 5 and 6 in Simulating Diurnal Cloud Cycle
Authors: Jiang Zhiye , An Yahan , Yin Jun
Published: March 2024

Cloud dynamics and their response to future climate change continue to present a significant source of uncertainty in climate predictions. Besides the average cloud properties, the diurnal cloud cycle (DCC) exerts a substantial influence on Earth’s energy balance by reflecting solar radiation during the daytime and continuously absorbing and reemitting longwave radiation throughout the whole day. Previous studies have demonstrated that climate models exhibit certain discrepancies in simulating the DCC; however, less research attention has been paid to the patterns of these DCC biases and their impacts on modeling the Earth’s energy balance. Here, we employ satellite data to compare DCC patterns in Coupled Model Intercomparison Project Phase 5 (CMIP5) and their latest versions in CMIP6 at both regional and global scales. We found that some of the latest climate models tend to have larger DCC biases when using satellite observations as the references, and the radiative effects due to DCC changes account for nearly 50% of the changes in total cloud radiative effects (CREs), suggesting that the DCC biases play a significant role in modelingthe global energy budget. We therefore call for improving cloud parameterization schemes with particular attention to their diurnal cycle to reduce their impacts on future climate projections.
Characteristics and Formation Mechanism of Ozone Pollution in Demonstration Zone of the Yangtze River Delta, China
Authors: Wu Yezheng , Gu Jun , Shi Xurong , Shen Wenyuan , Zhang Hao , Zhang Xin
Published: March 2024

Emerging research indicates that ground-level ozone (O3) has become a leading contributor to air quality concerns in many Chinese cities, with the Yangtze River Delta (YRD) region facing particular challenges. This study investigated the characterization of air pollutants in Wujiang, which is located within the YRD demonstration zone, during the warm season (April–September) of 2022. The contributions of emission and meteorology to O3 were identified, the O3-NOX-VOC sensitivities were discussed, and the VOC sources and their contributions to O3 formation were analyzed. A random forest model revealed that the high O3 concentration was mainly caused by a combination of increased emission intensity due to the resumption of work and production after the COVID-19 pandemic, along with adverse meteorological conditions. The results revealed more than 92% of the pollution days were related to O3 during the warm season, and the impact of O3 precursor emissions was slightly greater than that of the meteorological conditions. O3 formation was in the VOC-limited regime, and emission reduction strategies targeting VOCs, particularly aromatics such as toluene and xylene, have been identified as the most effective approach for mitigating O3 pollution. Changes in O3-NOX-VOC sensitivity were also observed from the VOC-limited regime to the transitional regime, which was primarily driven by variations in the NOX concentrations. The VOC source analysis results showed that the contributions of gasoline vehicle exhaust and diesel engine exhaust (mobile source emissions) were significantly greater than those of the other sources, accounting for 20.8% and 16.5% of the total VOC emissions, respectively. This study highlights the crucial role of mobile source emission control in mitigating O3 pollution. Furthermore, prioritizing the control of VOC emission sources with minimal NOX contributions is highly recommended within the VOC-limited regime.