bluuebunny/crossref_metadata_2025 huggingface dataset:
Prática interdisciplinar sobre alimentação utilizando a metodologia de Rotação por Estações na educação infantil
Authors: Gonçalves Carolina Bento Pereira , Soares Gisele Lángaro
Published: January 2021

Em dias atuais, a chamada era digital influencia todos os jovens, tanto na maneira de agir como de ver o mundo, de socializar e de aprender. As escolas ainda presas no método tradicional de ensino, sem acesso à tecnologia digital ou a nenhum outro aparato ou metodologia tecnológica, necessitam quebrar esse paradigma e incluir em seus processos métodos de ensino inovadores, que melhorem de forma significativa o aprendizado das crianças. Este artigo tem como objetivo avaliar uma prática de ensino interdisciplinar utilizando a técnica de Rotação por Estações para trabalhar o tema de alimentação saudável, abrangendo os componentes curriculares Português, Inglês e Ciências. Para isso, foi desenvolvido um plano de atividade, o qual foi posteriormente aplicado aos alunos do pré-escolar de uma escola particular no município de São Gabriel, RS. Trata-se de uma pesquisa-ação, com observação participante e com análise de documentos produzidos pela professora e coordenadora da escola. A análise dos resultados evidencia os benefícios da uma atividade interdisciplinar, bem como o aumento da participação e curiosidade dos alunos. Trabalhar conteúdos pedagógicos de forma interdisciplinar fornece recursos para que as crianças desenvolvam o ensino aprendizagem de forma autônoma, além de melhorar a interação com o grupo e a dinâmica em sala de aula. O uso da técnica de Rotação por Estações confirmou que um processo de aprendizagem se realiza de fato quando o aluno aprende de forma mais personalizada e autônoma com a valorização de suas habilidades e competências e tendo o professor como facilitador do processo de aprendizagem
Estudo da composição gravimétrica dos resíduos sólidos de uma escola pública do município de Santarém-PA
Authors: Assis Emily Cristy de Souza , Santos Jessyca Nepomuceno dos
Published: January 2021

Este estudo procurou conhecer e analisar a situação dos resíduos sólidos em uma escola pública. Trata-se de uma pesquisa quali-quantitativa, realizada a partir da aplicação de questionários com os funcionários e discentes da instituição. Além disso, foi realizada a caracterização física dos RS produzidos na escola, através da gravimetria. Estima-se que são gerados cerca 10,1 kg /dia, onde são gerados grandes quantidades de papeis e plásticos, caracterizados pela gravimetria de massa e volume. Em termos gerais, boa parcela da massa de RS corresponde a materiais que podem ser reciclados ou reutilizados (52,7%). A partir da análise dos resultados, foi possível identificar falhas no sistema de gerenciamento de RS, tais como: falta de segregação na fonte e resíduos armazenados de modo inapropriado. Conclui-se com recomendações para o aprimoramento do gerenciamento dos resíduos, dentre as quais recomendações referentes à realização de programas de educação ambiental (EA), visando à conscientização e sensibilização, em busca da mudança de atitudes em relação ao manejo dos RS, tanto na escola, quanto em ambiente extraescolar.
El archivo de la “Fundación Rodríguez”. Análisis, potencia y gestión del legado
Authors: Fidalgo Alday Iker
Published: January 2021

El presente artículo parte de un caso de estudio concreto que es la investigación en torno al archivo del colectivo artístico “Fundación Rodríguez” (Vitoria-Gasteiz, 1994-2012). El proceso de investigación estará marcado por todo lo que conlleva enfrentarse a un archivo compuesto por contenido digital y con formatos a punto de la obsolescencia, así como las dificultades para su conservación y mantenimiento. Partiendo de esto, se contextualizará la práctica de “Fundación Rodríguez” y el papel que juega el concepto de archivo en varias de las fases de su producción artística. Para ello analizaremos su posición desde el trabajo colectivo, la disolución del rol del artista y la desmaterialización de la obra artística como los tres frentes principales desde los que se erige su producción. Con todo, podremos valorar desde la actualidad la vigencia y relevancia de su legado, así como la potencia del mismo en el contexto artístico al que pertenece.

This article starts from a concrete case study that is the research around the archive of the artistic collective “Fundación Rodríguez” (Vitoria-Gasteiz, 1994-2012). The research process will be marked by all that is involved in dealing with an archive composed of digital content and formats on the verge of obsolescence, as well as the difficulties of preserving and maintaining them. Starting from this, the practice of “Fundación Rodríguez” and the role played by the concept of archive in several of the phases of its artistic production will be contextualized. To do so, we will analyze its position from the perspective of collective work, the dissolution of the role of the artist and the dematerialization of the artistic work as the three main fronts from which its production is built.With all this, we will be able to evaluate from the present time the validity and relevance of his legacy as well as its power in the artistic context to which it belongs.
Los ‘apocalipsis silenciosos’ del arte contemporáneo como dispositivos transformadores de conciencia para la reparación de una memoria ecológica degradada
Authors: González-García Ricardo
Published: January 2021

Como una reunión simbólica de conciencia, toda gran obra de arte es un apocalipsis silencioso que, con sus significativas impresiones o interacciones, puede llegar a transformar la estructura social. Transfigurando el mundo mediante sus espacios de representación, es capaz de adquirir una capacidad profética que denuncia situaciones a fin de, como en el caso específico aquí ofrecido, reestablecer el equilibrio de los ecosistemas degradados por la huella que el ser humano ha impreso sobre ellos. Esta misma impronta antropocénica, auspiciada por la idea de progreso que confiere la utopía del crecimiento ilimitado propuesta por el sistema capitalista, paradójicamente podría llevar al ser humano a asistir al fin de su propia especie. Por esta razón, parte del arte contemporáneo lleva tiempo sumamente preocupado en concienciar a la sociedad y, así, frenar la llegada de un catastrófico escenario futuro. Debido a la urgencia de esta acuciante situación, se abordan diversas denuncias establecidas en las prácticas artísticas para cambiar la actitud de sus espectadores. Para ello, se proponen aquí dos vertientes: la de obras que muestran escenarios distópicos y la de otras más activistas que tratan de atajar la situación desde entornos concretos.

As a symbolic gathering of conscience, every great artwork is a silent apocalypse that, with its significant impressions or interactions, can transform the social structure. Transfiguring the world through its spaces of representation, it is capable of acquiring a prophetic capacity that denounces situations in order to, as in the specific case offered here; restore the balance of ecosystems degraded by the footprint that human beings have printed on them. This same anthropocenic imprint, sponsored by the idea of progress that confers the utopia of unlimited growth proposed by the capitalist system, paradoxically could lead the human being to attend the end of his own species. For this reason, part of contemporary art has been extremely concerned about raising awareness in society and, thus, curbing the arrival of a catastrophic future scenario. Due to the urgency of this pressing situation, we complaints various complaints established in artistic practices to change the attitude of its spectators. To do this, we proposed two aspects here: that of works that show dystopian scenarios and that of other more activists which try to tackle the situation from specific environments.
VARIABLES RELACIONADAS CON EL NÚMERO Y ELECCIÓN DE LAS REDES SOCIALES DE LOS HOTELES ANDALUCES DE 4 Y 5 ESTRELLAS
Authors: Rabadán-Martín Inmaculada , Padilla-Garrido Nuria , Aguado-Correa Francisco
Published: January 2021

La gestión de las redes sociales se ha convertido en un factor clave para el turismo. Los contenidos generados en las mismas pueden condicionar la elección por parte de los turistas, la reputación online, las ventas, e incluso la supervivencia de un establecimiento hotelero. A través del estudio de 503 hoteles andaluces de 4 y 5 estrellas, nuestro trabajo analiza la totalidad de redes sociales presentes en sus páginas web oficiales, así como los posibles factores (número de estrellas, modalidad, antigüedad, tamaño y pertenencia a cadena) que pueden influir en el número y la elección de estas.

 

Social network management has become a key factor in developing tourism. Content generated on social networks can influence tourist choices, online reputation, sales and the very survival of the hotel establishment.

In a study of 503 four- and five-star hotels in Andalusia, we analysed all the social networks used on their official websites to attract customers, and the potential factors (number of stars, modality, age of the establishment, size, chain hotel or otherwise) that could influence the number of visits and choice of destination.
GESTIÓN SOSTENIBLE DE LOS HERITAGE SITES CULTURALES: PROPUESTA DE UN MODELO
Authors: Fresneda Fuentes Silvia , De Fuentes Ruíz Pilar , Lobo Gallardo Antonio
Published: January 2021

La gestión sostenible de los bienes de patrimonio histórico demanda sistemas de control de gestión que contemplen sus particularidades. El objetivo de este trabajo es el diseño de un modelo de control holístico, contingente e integrador a emplear por los responsables públicos y/o privados de su gestión. Se ha realizado una profunda revisión bibliográfica de la literatura. El modelo propuesto no pretende ser universal, sino un punto de referencia para su adaptación a las particularidades propias de cada bien. Incluye indicadores que permitirán medir y valorar el logro de los objetivos.

The requirement of a sustainable management of the historical heritage sites demands the design of management control models that contemplate the particularities of those sites, beyond the architectural ones. The goal of this paper is the design of a holistic, contingent and integrator model to be used by the public and/or private managers for the sustainable management. To achieve this, a thorough bibliographic review of the specialized literature has been carried out. This model does not pretend to be universal but rather a point of reference for its adaptation to the particularities of each site.
EL STORYTELLING EN LA MARCA DE DESTINOS TURÍSTICOS: EL CASO DE GIRONA
Authors: Camprubí Raquel , Planas Cèlia
Published: January 2021

El storytelling como estrategia para transmitir los valores e identidad de una marca ha adquirido importancia en los últimos años, pero aún hay poca literatura que desarrolle este concepto entorno a los destinos turísticos. Este artículo pretende analizar las potencialidades y uso del storytelling en un destino turístico a través de relatos y leyendas tradicionales. Las leyendas de la ciudad de Girona (España) se utilizaron como caso de estudio. Los resultados ponen en evidencia que, a pesar del potencial de las leyendas, actualmente no se está explotando adecuadamente.

Storytelling as strategy to transmit values and identity of a brand has acquired relevance during the last years. However, this concept is already relatively underdeveloped in academic literature on tourism destinations. This paper aims to analyze storytelling potentialities and use in a tourism destination trough traditional stories. Legends of the city of Girona (Spain) have been chosen as a case study. Results demonstrated that legends of the city have the potential to develop a storytelling strategy, but this potential is not used properly.
Guds børn og Fadervor (I):
Authors: Pedersen Nils Arne
Published: January 2021

In the baptismal ritual of the Church of Denmark, the Lord’s Prayer has since 1912 been placed after baptism while it formerly was placed before, as in Luther’s Taufbüchlein. Two consecutive articles argue that the replacement in 1912 was influenced by the theology of Nikolai Frederik Severin Grundtvig. The current first article deals with Grundtvig’s understanding of baptism as a sacrament, and his view and criticism of the baptismal ritual of his time.
Ydre mission som dansk kulturhistorie
Authors: Iversen Hans Raun , Iversen Hans Raun
Published: January 2021

In his new book, Daniel Henschen studies the foreign mission movement in Denmark as a case of an “empathic globalization” before modern globalization began to be developed at full scale from around 1960. The book is innovative in viewing the mission movement in Denmark as an integrated part of contemporary culture, contributing to the general cultural and political development in its time. At the same time, Henschen develops a new track in mission studies.
Guds børn og Fadervor (II)
Authors: Pedersen Nils Arne
Published: January 2021

In the baptismal ritual of the Danish Church, the Lord’s Prayer has since 1912 been placed after baptism while it formerly was placed before, as in Luther’s Taufbüchlein. Two consecutive articles argue that the replacement in 1912 was influenced by Nikolai Frederik Severin Grundtvig’s theology. The present second article deals with the different translations of Biblical passages central to the baptizee as a child of God, and attempts to demonstrate that Grundtvig identified the Lord’s Prayer with the Abba-cry mentioned in Romans 8:15 and Galatians 4:6. Thus, the Lord’s Prayer had its role to play after baptism and furthermore functioned for the believer as a daily confirmation and an inner assurance of salvation.
Daniel mellem to verdener: Diasporamotiver i Daniels Bog 1-6
Authors: Poulsen Frederik
Published: January 2021

The book of Daniel is set in the diaspora. As a Jew in Babylon, Daniel exists between two worlds: the culture of the host land and the values of his ancestral land. This article focuses on the tension between these two in the court legends (chs. 1-6). I argue that the relation between the two worlds is a dynamic one that is construed differently in the single stories. In my reading of the six stories, I demonstrate that the consistent use of irony, satire, and utopia points to a fundamental ambiguity of diasporic existence.
Reading spaces
Authors: Linkis Sara Tanderup
Published: January 2021

Following ‘the audiobook boom’ of recent years, born-audio narratives have emerged: texts produced specifi cally for the audiobook format and intended for mobile audio consumption. Focusing on this category of works, this article examines how the audiobook draws attention to places and situations in which we read, and how these places, in turn, infl uence the content and experience of literary works. Drawing on theories on mobile reading and listening by, for example, Michael Bull (2007), Lutz Koepnick (2013, 2019), and Iben Have and Birgitte Stougaard Pedersen (2015, 2020), I investigate the case of Storytel Originals, texts produced specifi cally for sound by the Swedish subscription service Storytel. Focusing first on the Danish Originals series Askehave (2019-2020) by Jakob Melander, I examine how Storytel promotes a situated reading experience for a mobile listener. Next, I move on to investigate what happens to the audiobook experience when the listener is not mobile: Cecilia Garme’s Original series Dagbok Från Coronabubblan (2020) describes everyday life during the corona crisis in spring 2020. Analysing the diary’s refl ections on the isolation at home and the listeners’ response to this text, I examine how the audiobook produces a social and intimate listening space. Based on these two examples, I point to two different tendencies in the content and usage of original audiobooks, one refl ecting how mobile listening promotes situated reading experiences in public and another focusing on the construction of social reading spaces at home.
Literature’s listening spaces
Authors: Vilmar Therese Wiwe
Published: January 2021

Literary descriptions of music are – of course – pure fi ction. However, such narratives are also windows into the phenomenological and sociological workings of music in modern society. Many novels share detailed descriptions of music in their fi ctional worlds, and this article examines what two contemporary novels reveal about modern-day music listening as both a cultural and private practice. The article will analyse the nature of ‘listening spaces’ represented in A Visit from the Goon Squad by Jennifer Egan (2010) and Kafka on the Shore by Haruki Murakami (2005). Both novels have been published within the fi rst decade of the 21st century and describe Western popular music. Music experienced by fi ctional characters can be valuable empirical data, because novels represent different listening situations varied by geography, epochs and genres, and they depict characters with different demographics, lives and musical/cultural backgrounds. This enables scholars to collect and compare multi-faceted datasets. The aim of this article is to use literary descriptions to ask qualifi ed questions about sociological and phenomenological aspects of contemporary music listening practices. The analysis will focus on the atmosphere of listening (Böhme, 2017) – and especially the fi ctional listeners’ bodily presence in musical spaces – in dialogue with sociological studies of music listening by especially Tia DeNora (2000), David Hesmondhalgh (2013) and Even Ruud (2013). The analysis indicates how fi ction articulates a connection between music, body (in space and place) and mind (emotions, temporality and memory).
Listening from the in-between
Authors: Cooper Ed
Published: January 2021

With homelessness being prevalent across the United Kingdom and showing no sign of decreasing, it is imperative to better understand the experiences of individuals who fall into these diffi cult circumstances. A previously unexplored aspect of homelessness is engagement with sound. This article addresses this lacuna by investigating how the understanding of sonic space is related to individuals’ experiences of homelessness. The article considers homelessness through the analytical lens of ‘liminality’—a period when an individual or space has neither a former nor future identity, whilst simultaneously, paradoxically, possessing both (van Gennep, 1960). Taking a phenomenological approach, interviews were undertaken with residents of a halfway house in Leeds, UK, whose circumstances are between ‘literal homelessness’ and social housing. The study demonstrates the ways in which participants actively engage with sound and liminality in day-to-day life, regularly curating inhabited sonic environments which are often seen by members of ‘mainstream’ society as ‘non-places’. A distinction is made between quietness and silence: whereas quietness offered the participants an escape, the prospect of silence—being left alone with one’s thoughts—was often worrisome. Further differentiation is made between actively ‘listening to’ and ‘hearing’ (Oliveros, 2005) these individual sonic spaces—the participants’ focus is positioned between external sonic stimuli and their own internal thoughts, highlighting a betweenness of consciousness. Overall, the article fi nds that interactions with sound are key components of the liminal experience of homelessness.
“Partisan requiem” by H. Sommerro: the peculiarities of interaction between liturgical and author’s verses in the context of the musical drama
Authors: Devyataikina Nina Ivanovna , Lyashova Svetlana Aleksandrovna
Published: January 2021

The research object is the sacred music of a well-known Norwegian composer Henning Sommerro (born 1952), one of the bright components of his multifaceted creative work. The present article is the first work within the Russian scientific literature analyzing this aspect through the prism of one of the key works of the composer. The authors are also the first to introduce the complete musical score of the composition into the scientific discourse. Along with the recordings, it helps to understand how the composer manages to successfully combine the liturgical and secular components, the poetical and musical texts, and how they outline the modern musical directions. The research subject is the musical drama and expressive means of the “Partisan Requiem” (2000) by Sommerro performed by the combined Russian-Norwegian choir (Murmansk, 2019). The research shows that the composer keeps the graphic structure of the historically formed model of the genre. Owing to the musical expressive means and “citation”, demonstrating the postmodernist practices of the composer, the genre becomes recognizable, denoting the important “memory spaces”. At the same time, in terms of drama, Latin and Norwegian texts are “arranged” according to the internal connection, the monologue inclusions of the narrator are often accompanied with a reduced orchestra, whose timbre and color, on the one hand, thematically unite the Norwegian texts with the graphic and musical sphere of the composition, and on the other hand, deepen the understanding of the ideas underlying them. The briefness of poetic verses strengthens the importance of each of them; they are rhythmically linked to liturgical texts thus helping to create a single lexical and musical fabric of the composition by means of a complex interaction of the choir, the orchestra, the soloists and the actor-narrator.   
Academic music transcript modeling using electronic sound synthesis
Authors: Kibitkina Elina Vadimovna , Fatianova Elena Alekseevna
Published: January 2021

Nowadays, computer technologies are integrated into all art forms. New artistic directions emerge which are totally based on multimedia (primarily audio-visual) instruments. The question about the role of music art in the new cultural paradigm becomes topical. Based on the example of academic music, the article considers the possibilities of using programming tools for music scores processing into a transcript. The authors consider the possible mathematical model of a piece of music which allows processing the piece of music and getting both an authentic and an experimental version. The authors analyze the set of parameters helping to form a virtual acoustic space corresponding with an artistic concept. The authors demonstrate that academic music transcript modeling using electronic sound synthesis can promote the artistic reinterpretation of the score of the piece of music. The selection of particular timber, performance and spatial parameters during transcribing helps to interpret a composition according to the artistic tasks. The use of virtual instruments also allows creating digital recordings without performing musicians. The article contains the variants of forming a virtual acoustic environment based on the example of a classical piece of music for string quartet: from a “traditional” to an “experimental, differing in musical sources positioning, a set of spatial effects and the parameters of their adjustment.   
The reconstruction of the “Naughty Couplets” ballet of the choreographer G. Malkhasyants
Authors: Melovatskaya Anna
Published: January 2021

The research subject is the period of the 1960s - the 1970s - the period of flourishing of the Soviet ballet theatre. During this period, in many theatres of the Soviet Union,  choreographers-pioneers were working whose creativity was based on the commitment to traditions and readiness to propose their own vision of the development of ballet theatre. The article considers the early period of work of one of “the men of the sixties” - a choreographer Gennady Malkhasyants (1937 - 2008). The author of the article reconstructs one of his earliest plays - the ballet “Naughty Couplets” staged in 1974 in Voronezh Opera and Ballet Theatre. The ballet was a part of an one-act ballet recital. The novelty of the research consists in the fact that it introduces into the scholar discourse the materials of the personal archive of the choreographer. The topicality of the research consists in the necessity to analyze the creative work of choreographers-directors, to reconstruct the plays which are not performed any longer and haven’t been recorded. In the 1970s, G. Malkhasyants was developing various choreographer’s techniques and director’s principles of the composition of a dance. So far, the creative work of G. Malkhasyants almost hasn’t been studied. The knowledge of the materials and the creative process of G. Malkhasyants gives the opportunity to trace back the main tendencies and, probably, to find the new ways of development of the modern art of choreography.   
P.A. Serebryakov’s performance art
Authors: Kameko Elena Mikhailovna
Published: January 2021

The research subject of the article is the piano art of Pavel Alekseevich Serebryakov, an outstanding Leningrad pianist, pedagogue, artist and director. The research object is the mani-sided activity of this person. The author considers such aspects of the topic and the performer’s individual style, his repertoire preferences, concert, music and social activity. The author gives special attention to particular facts of the great artist’s biography which had influenced his performance path. The author refers to the reviews of various musical critics, colleagues and students, as well as Serebryakov’s own performance views drawn from autobiographical notes and interviews. Based on the author’s conversation with P. Serebruakov’s grandson P.V. Dmitriev, the author reveals the methods of the pianist’s individual work with the repertoire and some peculiarities of his performance thinking. The scientific novelty of the research consists in the fact that it uses rare previously unpublished archive materials covering performance and music and social activity of P.A. Serebryakov, including: The Serebryakovs family archive documents provided by Serebryakov’s grandson P.V. Dmitriev Materials, reviews and newspaper articles kept at the Central State Archive of Literature and Arts (fund 214) Audio recordings from the sound library of St.Petersburg Conservatory Materials of Serebryakov’s conversations with students The research material can be used by young musicians, as well as the pedagogues of special music schools for illustrative and educational purposes.  
Longitudinal wave analysis of infinite length piezoelectric circular rod based on temperature effect
Authors: Chen Qiong , Xue Chun-Xia , Wang Xun ,   Department of Engineering Mechanics, School of Science, North University of China, Taiyuan 030051, China
Published: January 2021

Piezoelectric elements have been commonly used because of their wide applications in sensors, transducers, and some micro intelligent structures. However, in the fields of aviation, aerospace, and automation, some relevant equipment works in a harsh environment and is susceptible to the temperature change, thereby leading its performances to be greatly affected. Therefore, the problem of nonlinear wave relating to piezoelectric circular rods in different temperature fields is studied by modeling and numerical analysis. Firstly, based on the theory of finite deformation, we take infinite piezoelectric circular rod as a research object and consider the effects of transverse inertia and equivalent Poisson's ratio under the thermoelectric coupling action. Using the Hamilton principle and introducing the Euler equation, the longitudinal wave equation of piezoelectric circular rod is obtained. Secondly, Jacobi elliptic cosine function and Jacobi elliptic sine function expansion method are used to solve the wave equation of the piezoelectric circular rod, and the solitary wave solution and the exact periodic solution of the wave equation are obtained. It is found that the periodic solution can be reduced into a solitary wave solution under certain conditions, and it is proved theoretically that there may be solitary wave stably propagating in a piezoelectric circular rod. Finally, the dispersion curves of different wave velocity ratios and the curves about influences of temperature field on the waveform, amplitude and wave number of the piezoelectric rod are obtained by Matlab. The numerical results show that the wave velocity decreases with the increase of temperature when the wave velocity ratio is constant. Given the temperature is constant, it can be found that with the increase of the ratio, the amplitude of solitary wave gradually increases while the wavelength gradually decreases. In addition, the images obtained show that although temperature change can cause the characteristics of solitary waves to change, the solitary waves are always symmetrical bell shaped waves in the propagation process, reflecting the stability characteristics under the combined action of nonlinear and dispersion effects. Therefore, the variation of temperature field can influence and control some propagation characteristics of solitary waves. Moreover, the wave theory has been widely used in the nondestructive testing of structures and the improving of information transmission quality due to its special stability.
Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion
Authors: Angulo Guillem , Zeleznjak Jelena , Martínez-Vicente Pablo , Puñet-Ortiz Joan , Hengel Hartmut , Messerle Martin , Oxenius Annette , Jonjic Stipan , Krmpotić Astrid , Engel Pablo , Angulo Ana
Published: January 2021

Viral infections are controlled, and very often cleared, by activated T lymphocytes. The inducible co-stimulator (ICOS) mediates its functions by binding to its ligand ICOSL, enhancing T-cell activation and optimal germinal center (GC) formation. Here, we show that ICOSL is heavily downmodulated during infection of antigen-presenting cells by different herpesviruses. We found that, in murine cytomegalovirus (MCMV), the immunoevasin m138/fcr-1 physically interacts with ICOSL, impeding its maturation and promoting its lysosomal degradation. This viral protein counteracts T-cell responses, in an ICOS-dependent manner, and limits virus control during the acute MCMV infection. Additionally, we report that blockade of ICOSL in MCMV-infected mice critically regulates the production of MCMV-specific antibodies due to a reduction of T follicular helper and GC B cells. Altogether, these findings reveal a novel mechanism evolved by MCMV to counteract adaptive immune surveillance, and demonstrates a role of the ICOS:ICOSL axis in the host defense against herpesviruses.
Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cells
Authors: Glykofridis Iris E , Knol Jaco C , Balk Jesper A , Westland Denise , Pham Thang V , Piersma Sander R , Lougheed Sinéad M , Derakhshan Sepide , Veen Puck , Rooimans Martin A , van Mil Saskia E , Böttger Franziska , Poddighe Pino J , van de Beek Irma , Drost Jarno , Zwartkruis Fried JT , de Menezes Renee X , Meijers-Heijboer Hanne EJ , Houweling Arjan C , Jimenez Connie R , Wolthuis Rob MF
Published: January 2021

Germline mutations in the Folliculin (FLCN) tumor suppressor gene cause Birt–Hogg–Dubé (BHD) syndrome, a rare autosomal dominant disorder predisposing carriers to kidney tumors.FLCNis a conserved, essential gene linked to diverse cellular processes but the mechanism by whichFLCNprevents kidney cancer remains unknown. Here, we show that disruptingFLCNin human renal tubular epithelial cells (RPTEC/TERT1) activates TFE3, upregulating expression of its E-box targets, including RRAGD and GPNMB, without modifying mTORC1 activity. Surprisingly, the absence of FLCN or its binding partners FNIP1/FNIP2 induces interferon response genes independently of interferon. Mechanistically, FLCN loss promotes STAT2 recruitment to chromatin and slows cellular proliferation. Our integrated analysis identifies STAT1/2 signaling as a novel target of FLCN in renal cells and BHD tumors. STAT1/2 activation appears to counterbalance TFE3-directed hyper-proliferation and may influence immune responses. These findings shed light on unique roles of FLCN in human renal tumorigenesis and pinpoint candidate prognostic biomarkers.
Biological constraints on GWAS SNPs at suggestive significance thresholds reveal additional BMI loci
Authors: Hammond Reza K , Pahl Matthew C , Su Chun , Cousminer Diana L , Leonard Michelle E , Lu Sumei , Doege Claudia A , Wagley Yadav , Hodge Kenyaita M , Lasconi Chiara , Johnson Matthew E , Pippin James A , Hankenson Kurt D , Leibel Rudolph L , Chesi Alessandra , Wells Andrew D , Grant Struan FA
Published: January 2021

To uncover novel significant association signals (p<5×10−8), genome-wide association studies (GWAS) requires increasingly larger sample sizes to overcome statistical correction for multiple testing. As an alternative, we aimed to identify associations among suggestive signals (5 × 10−8≤p<5×10−4) in increasingly powered GWAS efforts using chromatin accessibility and direct contact with gene promoters as biological constraints. We conducted retrospective analyses of three GIANT BMI GWAS efforts using ATAC-seq and promoter-focused Capture C data from human adipocytes and embryonic stem cell (ESC)-derived hypothalamic-like neurons. This approach, with its extremely low false-positive rate, identified 15 loci at p<5×10−5 in the 2010 GWAS, of which 13 achieved genome-wide significance by 2018, including at NAV1, MTIF3, and ADCY3. Eighty percent of constrained 2015 loci achieved genome-wide significance in 2018. We observed similar results in waist-to-hip ratio analyses. In conclusion, biological constraints on sub-significant GWAS signals can reveal potentially true-positive loci for further investigation in existing data sets without increasing sample size.
Endothelial cell-type-specific molecular requirements for angiogenesis drive fenestrated vessel development in the brain
Authors: Parab Sweta , Quick Rachael E , Matsuoka Ryota L
Published: January 2021

Vascular endothelial cells (vECs) in the brain exhibit structural and functional heterogeneity. Fenestrated, permeable brain vasculature mediates neuroendocrine function, body-fluid regulation, and neural immune responses; however, its vascular formation remains poorly understood. Here, we show that specific combinations of vascular endothelial growth factors (Vegfs) are required to selectively drive fenestrated vessel formation in the zebrafish myelencephalic choroid plexus (mCP). We found that the combined, but not individual, loss of Vegfab, Vegfc, and Vegfd causes severely impaired mCP vascularization with little effect on neighboring non-fenestrated brain vessel formation, demonstrating fenestrated-vEC-specific angiogenic requirements. This Vegfs-mediated vessel-selective patterning also involves Ccbe1. Expression analyses, cell-type-specific ablation, and paracrine activity-deficient vegfc mutant characterization suggest that vEC-autonomous Vegfc and meningeal fibroblast-derived Vegfab and Vegfd are critical for mCP vascularization. These results define molecular cues and cell types critical for directing fenestrated CP vascularization and indicate that vECs’ distinct molecular requirements for angiogenesis underlie brain vessel heterogeneity.
Use of Essential Oil of Eucalyptus globulus Leaves against Sitophilus zeamais Motsch
Authors: Ngongo-Kapenga J. , Kwete S. Minga , Yefile-Mposhi S. , Kalonji-Mbangila A. , Mulumba-Badibanga G. , Ngombo-Nzokwani A. , Kalonji-Kabemba N. , Kalala B. Kalala , Kalonji-Mbuyi A. , Muengula-Manyi M.
Published: January 2021

Aim: To assess in laboratory conditions, the efficacy of essential oil of Eucalyptus globulus leaves against the infestation due to maize weevil Sitophilus zeamais, the main insect pest of stored maize grains.  

Study Design: This study was conducted as two separate trials. Each trial was performed using a completely randomized design with 5 treatments repeated 3 times.

Place and Duration of the Study: The study was conducted in the laboratory Unit of Phytopathology and Crop protection, Faculty of Agronomy, University of Kinshasa, between February and April 2018. 

Methodology: Two trials (in Petri dishes and in cotton bags) were conducted separately from each other, while treatments used were the same. Trials were conducted according to a completely randomized design with 5 treatments repeated 3 times. Treatments used corresponded to 5 different volumes (0, 15, 20, 25 and 30 ml) of essential oil of Eucalyptus globulus leaves. In each trial, data collected were the rate of S. zeamais mortality and percentage of maize grains damaged. 

Results: In general, statistical analysis showed significant differences (P<0.05) among treatments used. There was variation over time of the percentage of S. zeamais mortality as a function of the volume of essential oil of E. globulus. The volume of 30ml of essential oil caused higher mortality compared to other treatments. The percentage of maize grains damaged increased on control, while it decreased on maize grains treated with essential oil of E. globulus leaves.     

Conclusion: The results of the present study revealed that essential oil of E. globulus leaves can help to protect maize grains stored against infestation due to maize weevil, Sitophilus zeamais.
Evaluate the Effects of Potential Botanical and Conventional Insecticides on the Reproductive and Developmental Aspects of the Pest Agrotis Ipsilion (Lepidoptera: Noctuidae)
Authors: Gesraha Mohamed Ahmed , Ebeid Amany Ramadan
Published: January 2021

Background: Agrotis ipsilon (Hüfn.), is known as a hazardous destructive pest for corn, Zea mays (L.) in Egypt, where it negatively affects corn yield production. Using insecticides continuously were sharply affected the progress of pest development in the field. The frequent uses of chemical pesticides to protect field crops leads to the development of insecticide-resistant strains.

Aim: Evaluate the insecticidal effect of four plant extracts with insecticidal properties and a chemical insecticide to control A. ipsilon using biological parameters and fertility life table. Also, to estimate the effects of these insecticidal materials on population growth, developmental progress, and nutritional indices and hence make recommendation of the type of insecticides to be used in Integrated Pest Management Programs.

Methodology: 1- Tested materials: (1) chemical insecticide (Chlorfenapyr at 0.5% concentration). (2) Four plant extracts (petroleum ether extracts of both Melia azedarach and Vinca rosea, and alcohol & hexane extracts of Conyza aegyptiaca) at 5% concentration each.

2- Tested insect: Agrotis ipsilon (the egg stage and the 4th larval instar).

3- Bioassay: Eggs and larvae were treated with tested materials separately, then different biological parameters were calculated.

4- Life table parameters: Life table parameters were calculate using  the “Age-stage, two-sex life tables” computer program.

Results: The obtained data revealed that petroleum ether extracts of V. rosea and M. azedarach were the best antifeedant agents against the 4th larval instar of A. ipsilon. The female longevity was significantly shortened under the effect of both treatments of M. azedarach and Challenger insecticide. All tested materials reflected an effective decline in the female ability for laying eggs, where this effect was obvious in the case of M. azedarach and Challenger insecticide treatments. It was found that the Melia treatment prolonged the incubation and developmental periods for the immature stages leading to a reduction of some life table parameters of A. ipsilon and also increase the generation mean time.

Conclusion: The resultant data obtained in this work may help in the usage of plant extracts in the advancement of IPM programs for the greasy cutworm, (Agrotis ipsilon) in Egypt.
Agronomic Performance of Corn Cultivars in Low-Altitude in the Cerrado-Amazon Ecotone
Authors: Santos Weder Ferreira dos , Bequiman Lara Rythelle Souza , Maciel Lucas Carneiro , Peluzio Joênes Mucci , Júnior Osvaldo José Ferreira , Sodré Layanni Ferreira , Ferreira Talita Pereira de Souza , Cerqueira Fernando Barnabé , Faria Lucas Alves de
Published: January 2021

e of this study was to evaluate the agronomic performance of corn cultivars for grain production in the south at low altitude in the Cerrado-Amazon ecotone.

Place: The research was carried out at Sítio Vitória (8°18'32.0"S, 50°36'58.0"W, 278 MASL), in the south of the state of Pará, Brazil.

Study Design: The experimental design was randomized blocks with twelve treatments and three replications. The treatments were eight corn hybrids: AG 1051, AG 8088, BM 3051, BR 2022, BR 205, BR 206, BRS 3046 and PR 27D28; and four open pollination populations: AL BANDEIRANTE, ANHEMBI, CATIVERDE and M 274.

Methodology: Sowed on January 28, 2019. The following characteristics were evaluated: ear height, plant height, number of grains per row, ear diameter, ear length, ear weight and grain yield.

Results: The cultivars showed a difference for all traits. The grain yield of the cultivars ranged from 4,567 kg ha-1 (BR 205) to 9,450 kg ha-1 (AG 1051).

Conclusion: The hybrids AG 1051 and BM 3051 were the ones that stood out the most, had the best performance in the Cerrado-Amazon ecotone.
Assessing the determinants of soil fertility in cocoa-based (Theobroma cacao) agroforestry systems in the Littoral region of Cameroon
Authors: Tsufac Azembouh Roshinus , Awazi Nyong Princely , Yerima Bernard Palmer Kfuban
Published: January 2021

Soil fertility decline is a reality across the globe. Soil infertility has led to falling crop yields for both food and cash crops precipitating both poverty and food insecurity. Poor agricultural practices have often been blamed for declining levels of soil fertility. It was within this framework that this study was carried out to assess soil fertility levels in cocoa-based agroforestry systems in the Littoral region of Cameroon. Through the use of a mixed research approach and descriptive and inferential statistical analysis it was found that the most perceived indicators of soil fertility by cocoa farmers in cocoa-based agroforestry systems were cocoa yields (100%), soil colour (90%), indicator plant species (75%), presence of soil macro-organisms (80%), indicator weed species (65%), growth rate/vigour of the cocoa plant (70%),  and level of compaction of the soil (50%). Chi-square test statistic (X2 = 15.92, p<0.05) revealed that there was a statistically significant difference in cocoa farmers’ perceptions of soil fertility indicators in cocoa-based agroforestry systems. Most cocoa farmers perceived the level of soil fertility in cocoa-based agroforestry systems to be between average (29%) and low (43%). Very few cocoa farmers perceived that soil fertility level in cocoa-based agroforestry systems was very high (5.3%). Chi-square test statistic (X2 = 38.42, p<0.05), revealed the existence of a statistically significant difference in cocoa farmers’ perception of the level of soil fertility in cocoa-based agroforestry systems. Correlation and regression analyses showed the existence of a direct and inverse non-causal and causal relationship respectively between several explanatory variables and soil fertility in cocoa-based agroforestry systems. It was noticed that explanatory variables such as fertilizer/manure application, diversity of tree species in the cocoa agroforests,  diversity of soil macro-fauna in the cocoa agroforests, and density of soil macro-fauna in the cocoa agroforests, all had a statistically significant (p<0.05) direct non-causal and causal relationship with soil fertility in cocoa-based agroforestry systems. Meanwhile, explanatory variables such as age of the cocoa farm, density of cocoa plants in the cocoa agroforests, and application of agrochemicals in the cocoa agroforests, all had a statistically significant (p<0.05) non-causal and causal relationship with soil fertility in cocoa-based agroforestry systems. It is therefore recommended that there should be more organic fertilizer/manure application, more diversity of tree species in the cocoa agroforests, and measures to ensure the diversity and density of soil macro-fauna in cocoa-based agroforestry systems.
Morphological and Molecular Identification of Mycobiota Associated with Juniperus monosperma (Engelm.) Sarg. Blight in Mexico
Authors: Galindo-Cepeda Ma. E. , Sánchez-Arizpe A. , Ángel E. Castro-del , Velázquez-Morales S. F. , Arispe-Vázquez J. L.
Published: January 2021

The aim of the investigation was to identify the agents associated with blight in Juniperus monosperma at Lirios region, Arteaga, Coahuila. Botanical material was collected at Lirios, Arteaga, and taken to the laboratory. Pathogens were isolated in ADP culture medium and identifying by morphological criteria and molecular using primers ITS1 and ITS4. DNA extraction by the Dellaporta method, the visualization of obtained DNA was performed by electrophoresis on a 2% (p/v) agarose gel. DNA quantification was performed on a NanoDrop 1000 spectrophotometer, and DNA amplification was carried out in the Veriti thermocycler. Obtained sequences were aligned and compared with those available in the GenBank database of the National Center for Biotechnology Information (NCBI), using the BLAST algorithm (Basic Local Aligment Search Tool) to find conserved sequences. Pathogens were inoculated in stems of J. monosperma. The agents associated with blight were Alternaria sp., Aspergillus sp. and Rosellinia sp. and the sequence obtained compared with BLAST coincided only with Alternaria sp. and Aspergillus sp. with the access codes KP027305.1 and MG551283.1, respectively. According to data taken in field, Alternaria sp. behaved as the highest prevalence species and severity associated with blight in J. monosperma, and Rosellinia sp. in laboratory conditions.
Research Progress on Auricularia delicata
Authors: Xiao Li , Jacob Muharagi Samwel , Bo Zhang , Anran Xu
Published: January 2021

Aims: To describe the current status of A. delicata emphasizing on the key parameters; occurrence, classification, molecular studies, nutritional and medicinal benefits, cultivation status, and future development perspective.

Place and Duration of Study: China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020.

Methodology: In this study, various literatures were reviewed for each parameter studied. Findings were deduced from current literatures and discussed.

Results: The screening of bioactive contents of A. delicata revealed the presence of phenolic compounds; chlorogenic, flavonoids, and ethyl acetate, polysaccharides; Chitosan, fibers, β-glucans, mannans, chitin, and melanin. These substances exhibit hepatoprotective effects, antioxidant activities and antimicrobial activities against some microbes like Candida albicans, Bacillus subtilis, Enterococcus faecium, Streptococcus aureus, Bacillus cereus, Salmonella typhi and Escherichia coli. A. delicata also contains several nutrients namely; protein, vitamin B2, vitamin C, and minerals; Potassium, Calcium, Iron, Magnesium, Zinc, Manganese, that play a vital role in human growth and development. Moreover, its cultivation using various technologies provides an opportunity for high yield production. A. delicata was recently studied at Jilin Agricultural University and domesticated in Heilongjiang province in the northeastern part of China. Due to the similarity of its fruiting body with the structure of the deer tripe, it was assigned a common name as “Deer tripe mushroom”. A. delicata mycelia are capable of growing on several culture media with different nutritional profiles, optimal temperatures and pH values. It is cultivated under tropic temperatures ranging from 25°C-30°C, optimal pH of 6.5, and humidity 80-90%. The commonly used media include; Potato Dextrose Agar (PDA), Yeast Extract Agar (YEA) and Malt Extract Agar (MEA).

Conclusion: Therefore the above mentioned significant properties (occurrence, nutritional, medicinal, and cultivation) provide a foundation for further research on the development and utilization of A. delicata.