bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Cercopithifilaria bainae infection in shelter dogs and ticks in Oklahoma, USA
Authors: Lineberry Megan W. , Sundstrom Kellee D. , Little Susan E. , Stayton Erin M. , Allen Kelly E.
Published: March 2020

Background Cercopithifilaria bainae is a filarioid nematode of dogs. Infection with the parasite was not reported in the USA until 2017, when a dog with skin lesions in Florida was diagnosed. Brown dog ticks, Rhipicephalus sanguineus sensu lato, are the purported tick vectors, and are widespread in the USA. Therefore, C. bainae is likely present in additional states. Here, we tested dogs and ticks in Oklahoma for evidence of C. bainae infection. Methods Dermal punch biopsies were opportunistically collected from municipal shelter and client-owned dogs. Multiple skin samples collected from interscapular and head regions were tested by saline sedimentation to recover live microfilariae for morphometric identification and by PCR to amplify a 330 bp region of the filarioid 12S rRNA gene. Also, ticks observed on surveyed dogs were collected, identified to species level, and tested for filarioid DNA. Results A total of 496 saline sedimentations were performed on 230 shelter and 20 client-owned dogs. Cercopithifilaria bainae infections were identified in 2.6% (6/230) of shelter dogs by morphometry of microfilariae in sedimentations and/or amplification of DNA from skin. DNA sequences amplified from PCR positive skin samples were 99–100% identical to C. bainae reported in Italy. All skin samples from client-owned dogs were negative for filarioid infection by saline sedimentation and PCR. A total of 112 ticks, comprised of four species, were collected. Two of 72 R. sanguineus s.l., both engorged females found attached to a C. bainae infected dog, harbored C. bainae DNA (99–100% identity). One attached R. sanguineus s.l. male on the same dog harbored filarioid DNA sequence which was difficult to interpret at numerous base-pair locations, but was closest in identity (~80%) to C. bainae . Conclusions The distribution of C. bainae is more widespread than previously known. Here, we document C. bainae infections in dogs and DNA in brown dog ticks in Oklahoma for the first time. As brown dog ticks are commonly found throughout the USA, veterinarians in this region should consider C. bainae infection as a differential diagnosis in canine patients with dermatitis or polyarthritis.
Effects of Gastric Sleeve Surgery on the Serum Levels of GH, IGF-1 and IGF-binding Protein 2 in Morbidly Obese Patients
Authors: Al-Regaiey Khalid , Alshubrami Suad , Al-Beeshi Ibrahim , Alnasser Torki , Alwabel Abdulnasser , Al-Beladi Hassan , Al-Tujjar Omar , Alnasser Abdulrahman , Alfadda Assim , Iqbal Muhammad
Published: March 2020

Background Bariatric surgery is an effective treatment for severe obesity. It also ameliorates diabetes independently of weight loss through mechanisms that are not fully understood. In this study, we investigated the levels of GH, IGF-1 and IGF-binding protein (IGFBP-2) after gastric sleeve surgery in healthy obese individuals. Method This study was conducted in 33 obese (BMI >38.3) healthy male subjects aged 25 to 50 years undergoing sleeve gastrectomy. GH, IGF-1 and IGFBP-2 levels were evaluated by ELISA at baseline and 6-12 months after surgery. Other parameters, such as glucose, BMI, insulin, HOMA-IR and lipid profile, were also investigated. Results Systemic GH (12.32 vs. 50.97 pg/mL, p < 0.001) and IGFBP-2 levels (51.86 vs. 68.81 pg/mL, p < 0.001) were elevated after bariatric surgery. There was no change in IGF-1 level from before to after surgery. BMI (52.18 vs. 40.11, p = 0.001), insulin (19.35 vs. 8.80 mIU/L, p < 0.001) and HOMA-IR index (6.48 to 2.52, p < 0.001) were reduced after surgery. Lipid profile analysis revealed that total cholesterol (4.26 vs. 5.12 mmol/L, p < 0.001) and high-density lipoprotein (HDL) (0.90 to 1.55 mmol/L, p < 0.001) were increased, while triglycerides were decreased, after surgery (1.62 vs. 1.05 mmol/L p < 0.001). GH, IGF-1, and IGFBP-2 were not correlated with insulin or lipid parameters. Conclusions Our study suggests that improved circulating GH and IGFBP-2 levels may mediate the beneficial effects of gastric sleeve surgery in improving insulin sensitivity and reducing insulin demand.
Health-related quality of life among 13–14 year old adolescents with overweight - a mixed methods approach
Authors: Sundar Turid Kristin Bigum , Riiser Kirsti , Småstuen Milada , Opheim Randi , Løndal Knut , Glavin Kari , Helseth Sølvi
Published: March 2020

Background

Overweight and obesity are public concerns with risk of adverse health outcomes. Health-related quality of life (HRQoL) is lower in adolescents than children in general. An increase in body mass index (BMI) is associated with a decrease in HRQoL. The purpose of this study was to measure and explore the HRQoL among adolescents with overweight or obesity who had participated in an intervention study with the aim of increasing PA, reducing BMI and promoting HRQoL.

Methods

Mixed methods, with a convergent design, were used to investigate how different methodological approaches could expand our understanding of the adolescents’ HRQoL. Quantitative post-intervention data on HRQoL were collected among the 84 intervention participants, aged 13–14 years, using the KIDSCREEN 52 questionnaire. The data were compared with a Norwegian reference population of 244 individuals, and analysed using a non-parametric Mann-Whitney test. Qualitative semi-structured interviews were conducted with 21 adolescents from the intervention. A directed approach to content analysis was adopted, using the ten sub-scales from KIDSCREEN 52.

Results

HRQoL in the intervention sample was significantly reduced on the sub-scale of physical well-being compared to the reference population. The reference population scored significantly lower than the intervention sample on the sub-scale of parent relation and home life. No significant differences were found on the other sub-scales. The qualitative data supported the quantitative findings on the sub-scale of physical well-being, but showed that perceptions of fitness, energy level or health could vary. Regarding parent relations, the interviewees extended this to include relationships to other family members as equally important. Most of the interviewees expressed a negative view of their bodies, but not their clothing or accessories. This may explain why no statistically significant differences were found on these aspects in the results from the KIDSCREEN questionnaire.

Conclusion: The use of the KIDSCREEN 52 instrument gave important indications about the adolescents’ HRQoL. Combining methods enabled a comprehensive approach to research on HRQoL, indicating better ways of providing help. More research using the benefits of mixed methods approaches is needed to further elucidate these findings.
Transcriptome analysis reveals rapid defence responses in wheat induced by phytotoxic aphid Schizaphis graminum feeding
Authors: Zhang Yong , Fu Yu , Wang Qian , Liu Xiaobei , Li Qian , Chen Julian
Published: March 2020

Background

Schizaphis graminum is one of the most important and devastating cereal aphids worldwide, and its feeding can cause chlorosis and necrosis in wheat. However, little information is available on the wheat defence responses triggered by S. graminum feeding at the molecular level.

Results

Here, we collected and analysed transcriptome sequencing data from leaf tissues of wheat infested with S. graminum at 2, 6, 12, 24 and 48 hpi (hours post infestation). A total of 44,835 genes were either up- or downregulated and differed significantly in response to aphid feeding. The expression levels of a number of genes (9,761 genes) were significantly altered within 2 hpi and continued to change during the entire 48 h experiment. Gene Ontology analysis showed that the downregulated DEGs were mainly enriched in photosynthesis and light harvesting, and the total chlorophyll content in wheat leaves was also significantly reduced after S. graminum infestation at 24 and 48 hpi. However, a number of related genes of the salicylic acid (SA)-mediated defence signalling pathway and MAPK-WRKY pathway were significantly upregulated at early feeding time points (2 and 6 hpi). In addition, the gene expression and activity of antioxidant enzymes, such as peroxidase and superoxide dismutase, were rapidly increased at 2, 6 and 12 hpi. DAB staining results showed that S. graminum feeding induced hydrogen peroxide (H 2 O 2 ) accumulation at the feeding sites at 2 hpi, and increased H 2 O 2 production was detected with the increases in aphid feeding time. Pretreatment with diphenylene iodonium, an NADPH oxidase inhibitor, repressed the H 2 O 2 accumulation and expression levels of SA-associated defence genes in wheat.

Conclusions

Our transcriptomic analysis revealed that defence-related pathways and oxidative stress in wheat were rapidly induced within hours after the initiation of aphid feeding. Additionally, NADPH oxidase plays an important role in aphid-induced defence responses and H 2 O 2 accumulation in wheat. These results provide valuable insight into the dynamic transcriptomic responses of wheat leaves to phytotoxic aphid feeding and the molecular mechanisms of aphid-plant interactions.
Glucose transporter GLUT1 influences Plasmodium berghei infection in Anopheles stephensi
Authors: Wang Mengfei , Wang Jingwen
Published: March 2020

Background: Sugar feeding provides energy for mosquitoes. Facilitated glucose transporters (GLUTs) are responsible for the uptake of glucose in animals. However, the knowledge of GLUTs function in Anopheles mosquito is limited. Methods: Phylogenetic analysis of GLUTs in Anopheles stephensi (Asteglut) was performed by the maximum likelihood and Bayesian method. The spatial and temporal expression patterns of the four Astegluts were analyzed by qPCR. The function of Asteglut1 was examined using a dsRNA-mediated RNA interference method. Transcriptome analysis was used to investigate the global influence of Asteglut1 on mosquito physiology. Results: We identified 4 glut genes, Asteglut1 , Asteglutx , Asteglut3 and Asteglut4 in An. stephensi . Asteglut1, Asteglut3 and Asteglut4 were mainly expressed in the midgut. Plasmodium berghei infection differentially regulated the expression of Astegluts with significant downregulation of Asteglut1 and Asteglut4 , while upregulation of Asteglutx . Only knocking down Asteglut1 significantly increased the susceptibility of An. stephensi to Plasmodium berghei infection. This might be due to the accumulation of glucose prior to blood feeding in dsAsteglut1-treated mosquitoes. Our transcriptome analysis revealed that knockdown of Asteglut1 differentially regulated expression of genes associated with multiple functional clusters including detoxification and immunity. The dysregulation of multiple pathways might contribute to the increased P. berghei infection. Conclusions: Our study shows that Asteglut1 is essential in defense against P. berghei in An. stephensi . The regulation of Asteglut1 on vector competence might through modulating multiple biological processes, including detoxification and immunity.
Cost-effectiveness analysis of atezolizumab in advanced triple-negative breast cancer
Authors: Phua Lee Cheng , Lee Soo Chin , Ng Kwong , Aziz Mohamed Ismail Abdul
Published: March 2020

Background The IMpassion130 trial demonstrated that adding atezolizumab to nanoparticle albumin-bound (nab)-paclitaxel improved the survival of patients with untreated, advanced, programmed death ligand 1 (PDL1)-positive triple-negative breast cancer (TNBC). In view of the high cost of immunotherapy, it is important to examine its value with respect to both benefits and costs. In this study, the cost-effectiveness of atezolizumab/nab-paclitaxel combination therapy relative to nab-paclitaxel monotherapy was evaluated for the first-line treatment of advanced, PDL1-positive TNBC, from a healthcare system perspective. Methods A three-state partitioned-survival model was developed to compare the clinical and economic outcomes of treatment with atezolizumab/nab-paclitaxel combination therapy with nab-paclitaxel monotherapy in patients with advanced TNBC. Clinical data were obtained from the IMpassion130 trial and extrapolated to 5 years. Health state utilities were retrieved from the literature, while direct costs were sourced from public healthcare institutions in Singapore. The primary outcomes of the model were life years (LYs), quality-adjusted LYs (QALYs), costs and incremental cost-effectiveness ratios (ICERs). One-way and probabilistic sensitivity analyses and scenario analyses were conducted to explore the impact of specific assumptions and uncertainties. Results Adding atezolizumab to nab-paclitaxel resulted in an additional 0.361 QALYs (0.636 LYs) at an ICER of S$324,550 per QALY gained. The ICER remained high at $67,092 per QALY even when atezolizumab was priced zero. One-way sensitivity analysis showed that the ICER was most sensitive to variations in the cost of atezolizumab and the time horizon. Scenario analyses confirmed that the combination therapy was unlikely to be cost-effective even under extremely favourable assumptions. Conclusions Given the exceedingly high ICER, adding atezolizumab to nab-paclitaxel was unlikely to represent a cost-effective use of healthcare resources for the treatment of advanced PDL1-positive TNBC. Our findings will be useful in informing funding policy decisions alongside other considerations such as comparative effectiveness, unmet need and budget impact.
Perinatal listeriosis patients at a maternity hospital in Beijing, China
Authors: Li Chunyun , Zeng Huihui , Ding Xin , Chen Yi , Liu Xiaowei , Zhou Li , Wang Xin , Cheng Yumei , Hu Shanshan , Cao Zheng , Liu Ruixia , Yin Chenghong
Published: March 2020

Background: Listeriosis is a rare but severe emerging foodborne infectious disease. Perinatal listeriosis is extremely prone to causing septicaemia and CNS (central nervous system) infection, and serious adverse pregnancy outcomes (abortion and neonate death). This research reports perinatal listeriosis cases at a maternity hospital over the course of six years in Beijing, China.Methods: Beijing Obstetrics and Gynecology Hospital (BOGH) is the largest maternal and child health care hospital in China. We retrospectively searched all the laboratory confirmed 12 pregnancy-associated listeriosis cases during Jan 1, 2013 to Dec 31, 2018. The clinical manifestations, laboratory results, perinatal complications and outcomes were investigated.Results: In BOGH, 12 perinatal listeriosis cases were diagnosed based on Listeria positive culture, including 10 single pregnancies and 2 twin pregnancies, and the incidence of which is 13.7/100,000 deliveries (12/87,644). Among those cases, four pregnant women and four newborns had septicaemia, and two of the neonatal septicaemia also got CNS (central nervous system) infection. All the maternal patients recovered. For the fourteen offsprings, there were 8 newborns and 6 aborted fetuses, with two newborns died and 6 survived among the 8 newborns, and none of survivors had sequelae. The feto-neonatal fatality ratio is 57.1% (8/14).Conclusions: Perinatal listeriosis is associated with high morbidity and mortality. It has been confirmed that many factors contribute to the high mortality, such as high proportion of second trimester onset, empiric antibiotics with low pathogenic bacterium effective coverage.
Improvement on high-cholesterol diet induced atherosclerosis, lipid profile, oxidative stress and genotoxicity in the liver of mice by Echinops spinosissimus Turra subsp. spinosus
Authors: Dammak Donyez Frikha , Saad Hajer Ben , Bouattour Emna , Boudawara Ons , Jarraya Raoudha Mezghani
Published: March 2020

Background Hypercholesterolemia is a major risk factor for the development of atherosclerosis and endothelial dysfunction. Methods The present study investigates the possible mechanism of Echinops spinosissimus Turra subsp. spinosus ( E. s. spinosus ) flower on the high cholesterol diet. Results Our in vitro results demonstrated the richness of E.s. spinosus flower in antioxidant compounds, and its antioxidant activities. The co-administration of E.s. spinosus (100 or 200 mg/kg/day) with high-fat diet attenuated hepatotoxicity as monitored by the improvement of oxidative stress biomarkers and plasma lipid and liver parameters, when compared to the hypercholesterolemic mice. Atherogenic index and body weight were also reduced markedly, compared to control mice. These results were confirmed by the improvement of histological changes and DNA damage. Conclusion These data indicate that E.s. spinosus flower reduces the hypercholesterolemia risk and atherogenic properties of dietary cholesterol. Its hypocholesterolemic effect may be due to its antioxidant activities and its richness in bioactive molecule.
Failure to replicate the JTC-bias in patients with psychotic disorders: A secondary analysis of a baseline assessment of a randomized controlled trial
Authors: Pytlik Nico , Soll Daniel , Hesse Klaus , Moritz Steffen , Bechdolf Andreas , Herrlich Jutta , Kircher Tilo , Klingberg Stefan , Landsberg Martin W. , Müller Bernhard W. , Wiedemann Georg , Wittorf Andreas , Wölwer Wolfgang , Wagner Michael , Mehl Stephanie
Published: March 2020

Background: The jumping to conclusions bias (JTC) is considered to be an important causal factor in theoretical models on the formation and maintenance of delusions. However, recent meta-analytic findings show a rather equivocal pattern of results regarding associations between JTC and delusions. Thus, the aim of the present preregistered study is to investigate in a large sample whether the JTC-bias is more pronounced in patients with psychotic disorders in comparison to controls and whether the JTC bias is associated with more severe delusional conviction, persecutory delusions and positive symptoms in general. All hypotheses were pre-specified in the trial proposal.Methods: Patients with psychotic disorders (n = 300) enrolled in a therapy trial and healthy controls (n = 51) conducted a variant of the beads task (fish task) as a measure for the JTC-bias at start of the trial. Further, clinical interviews were used to assess patients’ delusional severity and delusional conviction.Results: There were no statistically significant differences between patients with psychotic disorders (with 53% displaying the JTC-bias) and controls (41%). Furthermore, there were no statistically significant correlations between JTC measures and persecutory delusions, delusional conviction and positive symptoms.Conclusions: We found no evidence for hasty decisions in patients with psychotic disorders, possibly because the fish task induces a more deliberative reasoning style as compared to the traditional beads task. The lack of associations between JTC measures and delusion severity is in line with meta-analytic findings using a wide range of JTC task variants. JTC is not as ubiquitous as previously thought and may only manifest itself in certain situational contexts. Trial Registration: ISRCTN29242879 (isrctn.com), date of registration: April 12th 2006, http://www.isrctn.com/ISRCTN12345678
Simplified Minimally Invasive Beating Heart Technique for Redo Isolated Tricuspid Valve Surgery
Authors: Lu Shuyang , Song Kai , Yao Wangchao , Xia Limin , Dong Lili , Sun Yongxin , Hong Tao , Yang Shouguo , Wang Chunsheng
Published: March 2020

BackgroundRedo isolated tricuspid valve surgery has been associated with a high morbidity and mortality, and its optimal timing of surgical intervention remains controversial. Hence, we reviewed our early and midterm results with a simplified minimally invasive beating heart technique for isolated redo tricuspid valve surgery in patients at high risk.MethodsBetween June 2016 and August 2017, a total of 14 consecutive patients underwent isolated tricuspid valve operations after previous cardiac operations with minimally invasive beating heart technique through a right lateral thoracotomy in our center. Mean patient age was 54.0 ± 8.3 years, and 9 patients (64.3%) were women. Mean preoperative EuroSCORE was 8.1 ± 1.3 (6 to 11). Previous cardiac operations included 6 patients (42.9%) with mitral valve replacement, 1 patient (7.1%) with mitral valve replacement and tricuspid valve repair, 1 patient (7.1%) with tricuspid valve replacement, 5 patients (35.7%) with mitral valve and aortic valve replacement, and 1 patient (7.1%) with Ebstein repair. Midterm follow-up was complete for 12 patients (85.7%).ResultsBoth in-hospital and thirty-day mortalities were 0%. Tricuspid valve replacement with bioprosthesis was performed in 12 patients (85.7%), and the remaining 2 patients (14.3%) underwent tricuspid repair (annuloplasty and leaflets reconstruction). Mean cardiopulmonary bypass time was 55.6 ± 10.7 minutes. Overall in-hospital duration and intensive care unit (ICU) time were 11.6 ± 8.8 days, 3.9 ± 2.8 days, respectively. Postoperative complications included 2 patients (1.4%) with prolonged ventilation, and 2 patients (1.4%) with acute kidney injury. There were no postoperative cerebrovascular accidents, myocardial infarctions, reoperations for bleeding, or deep wound infections. All patients were discharged uneventful. Except 2 patients lost follow-up, there were no adverse cardiovascular events and deaths occurred in other patients.ConclusionsSimplified minimally invasive beating heart technique for redo tricuspid valve surgery is both feasible and safe, and the early and midterm results are excellent.
Construction and analysis of the lncRNA-miRNA-mRNA network based on competitive endogenous RNA reveal functional lncRNAs in oral cancer
Authors: Yin Junhao , Zeng Xiaoli , Ai Zexin , Yu Miao , Wu Yang’ou , LI SHENGJIAO
Published: March 2020

Background There is a growing body of evidence suggesting that long non-coding RNAs(lncRNAs) can function as a microRNA(miRNA) sponge in various diseases including oral cancer. However, we are still not very clear about the pathophysiological function of lncRNAs. Methods We constructed a lncRNA-miRNA-mRNA network in oral cancer based on the competitive endogenous RNA(ceRNA) theory with the human expression profiles GSE74530 from the Gene Expression Omnibus (GEO) database and used topological analysis to determine the hub lncRNAs in the regulatory ceRNA network. Then, function enrichment analysis was performed by R package Cluster Profiler. Results A total of 238 potential co-dysregulated competing triples were obtained in the lncRNA-assoicated ceRNA network of oral cancer, which consisted of 10 lncRNA nodes, 41 miRNA nodes, and 122 mRNA nodes. Additionally, we found three lncRNAs( HCP5 , AGAP11 , HCG22 ) exhibiting superior potential as diagnostic and prognostic markers of oral cancer. Conclusions Our findings will provide novel insights for understanding the ceRNA regulation in oral cancer and identify three novel lncRNAs as potential molecular biomarkers.
Comprehensive analyses to identify LINC02310 as an enhancer in lung adenocarcinoma
Authors: Zhao Wenyuan , Wang Jun , Luo Qingxi , Peng Wei , Li Bin , Wang Lei , Zhang Chunfang , Duan Chaojun
Published: March 2020

Lung adenocarcinoma (LADC) is a major subtype of non-small cell lung cancer (NSCLC) and has one of the highest mortality rates. An increasing number of long non-coding RNAs (lncRNAs) were reported to be associated with the occurrence and progression of LADC. In order to find potential biomarkers for LADC therapies among lncRNAs, 2431 differentially expressed lncRNAs (DElncRNAs) over LADC and adjacent normal samples were identified from The Cancer Genome Atlas (TCGA) expression data. Lnc-YARS2-5, lnc-NPR3-2 and LINC02310, which were the top-3 significant DElncRNAs related to overall survival, were selected for further analysis. Their overexpression indicated poor prognostic. PCR experimental results also showed that they were mainly up-regulated in the LADC tissues from Xiangya Hospital. Clinical analysis of these cases suggested that LINC02310 was significantly correlated with TNM-stage and T-stage, which was further validated by the clinical analysis based on TCGA. Reasonably, we assumed that LINC02310 acted as an enhancer in LADC, which was demonstrated by CCK-8 assay and colony formation assay. Moreover, 3 targeted miRNAs of LINC02310 and 414 downstream differentially expressed mRNAs (DEmRNAs) were predicted. The competing endogenous RNA (ceRNA) regulatory network was constructed with LINC02310, 3 miRNAs and 113 DEmRNAs involved. The downstream mRNAs were then enriched in 405 GO terms and 11 KEGG pathways, which revealed their potential functions and mechanisms. The protein-protein interaction (PPI) network showed the relationships between the downstream mRNAs. In conclusion, this study verified LINC02310 as an enhancer in LADC and conducted comprehensive analyses on its target miRNAs and downstream mRNAs.
Up-regulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/β-catenin signaling pathway
Authors: Lu Fengqin , Li Chunhong , Sun Yuping , Jia Ting , Li Na , Li Haiyan
Published: March 2020

Mounting evidences displayed that miRNAs modulated glioblastoma (GBM) development. Here, our goal was to investigate whether miR-1825 could regulate GBM development and explore its potential mechanism. Our findings displayed that miR-1825 was decreased in GBM tissue specimens by qRT-PCR and it confirmed as a prognostic marker of GBM by Kaplan-Meier survival analysis. Moreover, we also found that miR-1825 up-regulation suppressed GBM cell viability, tumor growth, invasion and migration by MTT assay, Xenograft assay and Transwell assay. Furthermore, CDK14 was first identified as the target of miR-1825 by Luciferase reporter assay and CDK14 acted as an oncogene in GBM development by Immunohistochemistry. In addition, Western blot analysis demonstrated that Wnt/β-catenin signaling pathway took part in GBM development modulating by miR-1825. In conclusion, miR-1825 up-regulation suppressed GBM progression by targeting CDK14 through Wnt/β-catenin pathway.
Internet-based cognitive rehabilitation for WORking Cancer survivors (i-WORC): study protocol of a randomized controlled trial
Authors: Klaver Kete , Duijts Saskia , Geusgens Chantal , Aarts Maureen , Ponds Rudolf , Beek Allard van der , Schagen Sanne
Published: March 2020

Background Cognitive problems are common in non-central nervous system cancer survivors. These problems are perceived as an important contributor to decline in work performance and work ability. Various interventions for cognitive problems have been proposed, but effectiveness regarding work-related outcomes has not yet been established. Effective treatment options to alleviate the adverse influence of cognitive problems on work performance are needed for working cancer survivors. In this paper, we will describe the design of a randomized, controlled, multicenter trial that evaluates the (cost-)effectiveness of an internet-based cognitive rehabilitation program for occupationally active cancer survivors confronted with cognitive problems. Methods/ Design A three-armed randomized controlled trial will be conducted, including two intervention groups (i.e., basic and extensive cognitive rehabilitation program) and one waitlist control group. In total, 261 cancer survivors (18 – 65 years) who have returned to work, and who experience cognitive problems will be recruited. Patients with and without cognitive impairment as established in a neuropsychological assessment, will be eligible; stratification will take place based on the presence of this cognitive impairment. The extensive intervention arm will contain a comprehensive training program (including psycho-education, fatigue management, and cognitive strategy training) with individual guidance (blended intervention). The basic intervention arm will contain a brief cognitive training program (including psycho-education and fatigue management) without individual guidance. The primary outcome will be accomplishment of an individually defined work-related treatment goal. Secondary outcomes include, among others, subjective cognitive functioning, work functioning, and quality of life. Primary and secondary outcomes will be measured at baseline (T0), and at 12 weeks (T1) and 26 weeks (T2) post-randomization. Discussion About 40-50% of the cancer patients worldwide are of working age at time of diagnosis. Many of the occupationally active cancer survivors experience cognitive problems. Both from an individual and a societal perspective, it is important to sustain cancer survivors’ employability. An effective treatment to alleviate the impact of cognitive decline and to improve work ability might help cancer survivors to sustain employability. Trial registration The trial is registered at ClinicalTrials.gov (NCT03900806) at 03 April 2019 (current status: ongoing),https://clinicaltrials.gov/ct2/show/NCT03900806?term=NCT03900806&draw=2&rank=1. KEYWORDS Cancer-related cognitive impairment, Cognitive rehabilitation, Employment, Internet-based, Randomized controlled trial, Cost-effectiveness
Examining Long-Term Effects of Human Papillomavirus Vaccine Recommendation Messages: A 4-months Follow-up Survey of a Randomized Controlled Study in Japan
Authors: Okuhara Tsuyoshi , Ishikawa Hirono , Ueno Haruka , Okada Hiroko , Kiuchi Takahiro
Published: March 2020

Background: We previously conducted a randomized controlled study to examine persuasive messages recommending HPV vaccination to mothers with daughters in September 2017 in Japan. Our previous study showed that the three types of intervention message used (statistical information only, a patient’s narrative in addition to statistical information, and a mother’s narrative in addition to statistical information) all significantly improved mothers’ intention to have their daughter(s) receive the HPV vaccine, in comparison with mothers who received no messaging. The present study is a follow-up survey to assess the long-term effect of the intervention in our previous study. Methods: Four months after the initial study, in January 2018, participants in the previous study were contacted and queried about their current intention to have their daughter(s) receive the HPV vaccine. Statistical analysis was conducted using the paired t -test and analysis of variance. Results: A total of 978 mothers participated in the current survey. Vaccination intention 4 months after intervention had decreased to a level that did not differ significantly from the level prior to intervention in all three intervention conditions. The amount of change in vaccination intention 4 months after intervention did not differ significantly among the three intervention groups ( p =0.871). Conclusions: A single exposure to messaging was insufficient to produce a persistent intervention effect; repeated exposure to messaging coupled with other interventions may be important.
Lung ultrasound in patients with rheumatoid arthritis and the definition of significant interstitial lung disease
Authors: Carlo Marco Di , Tardella Marika , Filippucci Emilio , Carotti Marina , Salaffi Fausto
Published: March 2020

Background

In recent years, a growing interest has grown around interstitial lung disease (ILD) in patients with rheumatoid arthritis (RA). While high resolution computed tomography (HRCT) of the chest remains the diagnostic method of choice, increasing attention has been directed towards lung ultrasound (LUS) in the diagnosis of ILD in connective tissue diseases. However, it is not yet well defined how to interpret the LUS findings under suspicion of RA-ILD. The aim of this study was to determine the cut-off number of LUS B-lines that identifies a significant RA-ILD.

Methods

A cross sectional study was conducted on consecutive RA patients with suspected RA-ILD. The inclusion criteria were clinical (dyspnea, velcro sounds), instrumental (suggestive anomalies on conventional radiography, DLco reduction), or in presence of at least two of the following risk factors for RA-ILD: smoking habit, male sex, advanced age, and ACPA presence.
Patients underwent LUS (carried out in 14 defined intercostal spaces), chest HRCT, pulmonary function tests, and clinical evaluation. The diagnosis of RA-ILD was based on a semi-quantitative evaluation of chest HRCT using a computer-aided method (CaM). The discriminative validity of the LUS versus HRCT has been studied by using the receiver operating characteristic (ROC) curve analysis.

Results

72 consecutive RA patients (21 male, 51 female) were evaluated, with a mean age of 63.0 (SD 11.5 years). The mean estimate of pulmonary fibrosis using the CaM was 11.20% (SD 7.48) at chest HRCT, while at LUS the mean number of B-lines was 10.65 (SD 15.11). A significant RA-ILD, as measured by the CaM at HRCT, was detected in 25 patients (34.7%). The presence of 9 B-lines was found to be the optimal cut-off at ROC curve analysis. This LUS cut-off defines the presence of significant RA-ILD with a sensitivity of 70.0%, a specificity of 97.62%, and a positive likelihood ratio of 29.4.

Conclusion

The present study provided data to determine the number of B-lines to identify a significant RA-ILD. LUS may represent a useful technique to select RA patients to be assessed by chest HRCT.
MicroRNA-559 restrains gastric cancer progression via activating AKT signaling pathway by targeting TRIM14
Authors: Wei Li , Zhang Lu , Zhang Bo , Wang Guanghui , Meng Lei , Zhang Junbo , Meng Yan , Qin Hao
Published: March 2020

Background: MicroRNAs (miRNAs) act as pivotal functions in gastric cancer (GC) carcinogenesis and progression. MiR-559 has been defined as a potential cancer suppressor gene in a few cancers. Nevertheless, the biological effect of miR-559 in human GC and the underlying molecular mechanism still unclear and need to be further illuminated. Methods: Quantitative real-time PCR (qRT-PCR) was fulfilled for measuring the miR-559 expression level in GC. The dual-luciferase reporter was used to verify that tripartite motif-containing 14 (TRIM14) is a target gene of miR-559. The expression levels of TRIM14 were examined by qRT-PCR and Western blot in GC tissue specimens and cell lines. The effects of miR-559 on GC cell growth were detected with MTT and cell counting assays. Cell cycle and apoptosis were examined by using flow cytometry. Overexpression and siRNA further demonstrated the role of TRIM14 in GC. Results: Our results revealed that miR-559 expression was dramatically downregulated in human GC tissue specimens and cell lines. MiR-559 overexpression restrained GC cell growth, and induced cell cycle G1-S phase arrest and apoptosis. MiR-559 inhibitors promoted cell multiplication and cell cycle G1-S transition, and inhibited apoptosis. MiR-559 level was negatively related to TRIM14 mRNA level in GC. The results showed that TRIM14 was affirmed to be a target gene of miR-559. MiR-559 overexpression downregulated the TRIM14 expression, and miR-559 inhibitors upregulated the TRIM14 expression. Particularly, knockdown of TRIM14 gave rise to the semblable cellular effects observed upon miR-559 overexpression. TRIM14 overexpression recapitulated the revulsive cellular and molecular effects by suppression of miR-559. Furthermore, both miR-559 overexpression and TRIM14 silencing led to the inhibition of the AKT signaling pathway. On the contrary, miR-559 suppression and TRIM14 overexpression activated the AKT signaling pathway, and inhibited Bax/Bcl-2 pathway. Conclusions: The findings illustrate that miR-559 restrains cell multiplication via suppressing the AKT signaling pathway in human GC and induces cell apoptosis through the suppression of Bax/Bcl-2 signaling pathway via targeting TRIM14. The findings imply that miR-559 acts as a pivotal function in human GC and represents a latent novel target in GC therapy.
The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules.
Authors: Liu Caidong , Chen Hongling , Sun Tong , Wang Haibo , Chen Baoan , Wang Xuerong
Published: March 2020

Background Circulating cancer cells (CTCs) provide opportunities for early diagnosis and evaluation of cancer stage as a more acceptable non-invasive liquid biopsy. The advanced development and use of CTCs for diagnosis or prognosis just started in recent years. Methods Fifty three patients, diagnosed as SPNs with a diameter less than 30 mm by CT examination, was enrolled for statistical analysis based on their CTCs level, CT examination features, serum tumor marker concentrations, and histopathological characteristics. Centromere probe 8 (CEP8) was utilized as a marker for CTCs determination. Results The CTCs level was significantly different between malignant and benign SPNs, as well as between early (0/Ia) and advanced (Ib/II/III) stages of lung cancer. For the malignancy judgment and the pTNM stage diagnosis of SPNs, ROC analysis results showed that combined use of CTCs level or density feature of CT morphology significant improved diagnostic effect compared to use of these two markers solely. Moreover, in bigger, single, and solid SPNs based on CT morphology, the CTCs level significantly correlated with the malignant histopathology. Additionally, triple staining (CEP8, EpCAM and CKs) results using samples from 22 out of 53 patients showed that more CTCs was detected when CEP8 was used as a marker. Conclusion The CTCs determined by CEP8 positive would be a potential adjuvant diagnostic marker for the malignance and stage of lung cancer for patients with SPNs.
Managing the agricultural enterprises’ valuation: actuarial approach
Authors: Fomina Olena , Moshkovska Olena , Luchyk Svitlana , Manachynska Yulia , Kuzub Mikhaylo
Published: March 2020

This article aims at finding ways to improve the efficiency of Ukrainian agricultural enterprises’ valuation management based on a multidimensional actuarial model. The article confirmed the hypothesis of the relationship between accounting information, published in the financial statements of agricultural enterprises, compiled according to international financial reporting standards (IFRS), and actuarial management reporting. It is the latter, in the context of financial reporting conceptual basis, which promotes foreign investor’s managerial decisions on the expediency of investing temporarily free financial resources in the development of the agro-sector. The methodology of the research is based on optimization of the algorithm for estimating the agricultural enterprise market value within the framework of a cost-effective approach based on information filling of the actuarial management reporting using the method of adjusted net assets. The results of the study showed the dependence of investment attractiveness of Ukrainian agricultural enterprises on qualitative content of the reporting information provided within the framework of international accounting. The practical value of the results confirmed the optimal algorithm for estimating the value of agribusiness based on the accounting and information of actuarial management reporting according to its spatial interpretation from 3D to 5D format. The proposed 5D model for managing the value of agribusiness will facilitate an objective assessment of the agricultural enterprise value in just one step.
AcknowledgmentThe article has been prepared within the research project “Business Value Management” (state registration No. 0118U000131) implemented in the Kyiv National University of Trade and Economics.
Cárie dental no Espírito Santo: perspectivas para o controle e prevenção
Authors: Dantas A.
Published: March 2020

Cárie dental; Prevalência; Incidência; Placa dental; Fluoretos.
Ausência de sensibilização alérgica ao látex mediada por IgE em profissionais jovens da odontologia
Authors: Geller M. , Paiva T. C. B. , Geller P. , Wolf H. , Fidel S. R. , Fidel R. A. S.
Published: March 2020

Alergia ao látex; Dentistas; Epidemiologia; IgE; Testes de puntura.
Avaliação do risco à cárie em pacientes portadores de aparelho ortodôntico fixo
Authors: Fonseca L. , Campos D. M. K. S. , Shigetomi M. N. , Ito I. Y. , Brêtas S. M. , Valle M. A. S. , Miotto M. H. M. B. , Elias A. M.
Published: March 2020

Prevenção; Risco de cárie; Ortodôntia; S. mutans.
Saúde e desigualdades sociais: um tema revisitado a partir de uma investigação empírica
Authors: Tavares David
Published: March 2020

Introdução: A maioria dos estudos sobre este tema tem salientado a existência de uma forte relação entre as desigualdades sociais e as desigualdades em saúde. Objetivo: Revisitar o tema das desigualdades sociais em saúde nas últimas três décadas, a partir de uma investigação empírica realizada no Município de Loures, situado na Área Metropolitana de Lisboa, Portugal. Métodos: O universo de estudo foi constituído pela população do Município de Loures com 18 anos ou mais. Foi adotada uma metodologia quantitativa, centrada na aplicação de um inquérito a uma amostra representativa do Município em estudo, estratificada por sexo, idade e área de residência. Foram realizados 1100 inquéritos, por entrevista, em todas as freguesias do Município. Resultados: Os resultados revelam acentuadas assimetrias entre as classes sociais relativamente a diversos factores, como o tipo de acesso aos serviços de saúde (público/privado), às consultas de medicina dentária, bem como a adoção de práticas preventivas como o controlo do peso ou os cuidados particulares com a alimentação. Por outro lado, verificam-se práticas transversais às diferentes classes sociais, como a frequência regular de consultas e exames médicos, devido, em grande parte, à universalização do acesso aos serviços públicos de saúde. A mesma tendência de relativa transversalidade entre as classes sociais está igualmente presente na regularidade de consumo de medicamentos. Conclusão: O aprofundamento do estudo da relação entre as desigualdades sociais e as desigualdades em saúde pode constituir um suporte importante para a implementação de linhas de intervenção social e política sustentável.
Gestão na estratégia de Saúde da Família: avaliação das equipes participantes do 3º ciclo do PMAQ-AB em município de médio porte
Authors: Josiane Aleixo da Silva Siqueira  , Luciane Zanin  , Arlete Maria Gomes Oliveira  , Fabíola Holanda Barbosa Fernandez  , Flávia Martão Flório
Published: March 2020

Introdução: O Ministério da Saúde (MS) instituiu em 2011 o Programa Nacional de Melhoria do Acesso e da Qualidade da Atenção Básica (PMAQ-AB). Objetivo: Este estudo analisou a implementação do 3º ciclo do PMAQ-AB na cidade de Aracruz/ES, ocorrido no período de 2015 a 2016. Métodos: Abordagem qualitativa por meio da Análise de Conteúdo e estudo de caso foram adotados para investigar o fenômeno em questão. Foram realizadas 22 entrevistas envolvendo o Subsecretário de Saúde, Coordenadores do PMAQ-AB, Conselheiros Municipais de Saúde, Apoiadores Estaduais de Saúde, gerentes, dentistas e enfermeiros da ESF. As entrevistas e documentos oficiais foram analisados por meio da Condensação de Significados em duas categorias como eixos norteadores: formulação, implantação e implementação do PMAQ e percepção do programa. Resultados: Os entrevistados mostraram-se envolvidos e apontaram dificuldades decorrentes da sobrecarga de funções, da estrutura física das unidades, além da falta de material e de capacitação sobre o programa. Dentre as melhorias apontadas, aparecem a organização do processo de trabalho e as práticas desenvolvidas. As dificuldades apresentadas pelos entrevistados trazem à tona a falta de retorno em relação às avaliações e ao recurso financeiro advindo do programa. Conclusão: A experiência foi significativa, emergiram sugestões de melhorias em relação ao processo de avaliação e monitoramento da saúde e questionamentos quanto à falta de retorno sobre os resultados das avaliações e uso do recurso.
Restauração classe II, utilizando uma técnica ultra conservativa: relato de caso clínico
Authors: Loss Maria Domingas Covre , Masioli Marco Antônio , Cunha Deise Linha
Published: March 2020

Cárie dental; Dentística operatória; Modalidades de tratamento.
influência do acompanhante sobre o comportamento do paciente infantil durante o atendimento odontológico
Authors: Sanglard Peixoto Luciana Faria , Frauches Marileny Boechat , Costa Alexandre Sylvio
Published: March 2020

Acompanhante; Comportamento; Paciente infantil.
Análise in vitro da capacidade de preenchimento do sistema de canais radiculares promovido por duas técnicas de obturação
Authors: Santiago Maria Regina Japlassú , Alvares Gustavo Ribeiro , Szmajser Luciane Kac , Fidel Sandra Rivera , Fidel Rvail Antônio Sérgio
Published: March 2020

Técnicas de obturação; Selamento apical; Sistema de canais radiculares.
Técnicas auxiliares de manejo do medo e da ansiedade no atendimento odontopediátrico
Authors: Fröner Andrea Martins , Dutra Cláudia Augusta Viana , Righi Mariane Porto , Barata Juliana Sarmento
Published: March 2020

Medo odontológico; Ansiedade odontológica; Comportamento infantil; controle de comportamente; Consulta odontológica.
PD1 and PD-L1 Inhibitors for the Treatment of Kidney Cancer: The Role of PD-L1 Assay
Authors: Cimadamore Alessia , Massari Francesco , Santoni Matteo , Lopez-Beltran Antonio , Cheng Liang , Scarpelli Marina , Montironi Rodolfo , Moch Holger
Published: March 2020

Background:

Immune checkpoint inhibitors targeting the programmed death receptor ligand
1 (PD-L1)/programmed death receptor 1 (PD-1) pathway represent a drastic change in the treatment
landscape of RCC resulting in a dynamic and evolving scenario. There is an urgent need for predictive
biomarkers of response to provide a personalized therapeutic strategy for individual patients.

Objective:

In this review, we focused on trials that investigated the administration of a PD-1 and PDL1
inhibitor alone or in combination with another agent and compared the different assays applied in
each trial to evaluate the role of PD-L1 as a prognostic and predictive biomarker.

Conclusion:

So far, the use of PD-L1 expression alone is not sufficient to predict treatment response
and present many limitations: the lack of consensus between different methodologies on biomarker
assessment, the heterogeneity of PD-L1 between primary tumors and metastatic sites, different criteria
of response to therapy (RECIST vs. irRECIST), the complex interplay with inflammatory components,
previous treatments, administration of antibiotic therapy. Combinations of different biomarkers and
biological features, such as gene expression associated with angiogenesis, immune response and myeloid
inflammation are promising biological variables that need to be validated in the context of prospective
clinical trials.
Diabetes Mellitus and Osteoporosis Correlation: Challenges and Hopes
Authors: Ala Moein , Jafari Razieh Mohammad , Dehpour Ahmad Reza
Published: March 2020

Diabetes and osteoporosis are two common diseases with different complications. Despite
different therapeutic strategies, managing these diseases and reducing their burden have not been satisfactory,
especially when they appear one after the other.

In this review, we aimed to clarify the similarity, common etiology and possible common adjunctive
therapies of these two major diseases and designate the known molecular pattern observed in them.

Based on different experimental findings, we want to illuminate that interestingly similar pathways lead
to diabetes and osteoporosis. Meanwhile, there are a few drugs involved in the treatment of both diseases,
which most of the time act in the same line but sometimes with opposing results. Considering the
correlation between diabetes and osteoporosis, more efficient management of both diseases, in conditions
of concomitant incidence or cause and effect condition, is required.