bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatobiliary-Related Outcomes in US Adults Exposed to Lead
Authors: Obeng-Gyasi Emmanuel , Armijos Rodrigo , Weigel M. , Filippelli Gabriel , Sayegh M.
Published: April 2018

The purpose of this cross-sectional study was to investigate hepatobiliary-related clinical markers in Unites States adults (aged ≥ 20) exposed to lead using the National Health and Nutrition Examination Survey (NHANES) 2007–2008 and 2009–2010 datasets. Clinical markers and occupation were evaluated in 4 quartiles of exposure—0–2 μg/dL, 2–5 μg/dL, 5–10 μg/dL, and 10 μg/dL and over—to examine how the markers and various occupations manifested in the quartiles. Linear regression determined associations, and binary logistic regression predicted the likelihood of elevated clinical makers using binary degrees of exposure set at (2 μg/dL, 5 μg/dL, and 10 μg/dL). Clinical makers, and how they manifested between exposed and less-exposed occupations, were explored in addition to how duration of exposure altered these clinical markers. In regression analysis, Gamma-Glutamyl Transferase (GGT), total bilirubin, and Alkaline Phosphatase (ALP) were positively and significantly associated with Blood lead level (BLL). Using binary logistic regression models, at the binary 2 μg/dL level ALP, and GGT were more likely to be elevated in those exposed. At 5 μg/dL level, it was ALP and GGT that were more likely to be elevated in those exposed whereas at 10 μg/dL level, it was GGT that were more likely to be elevated in those exposed. In the occupational analysis, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), GGT, and ALP showed differences between populations in the exposed and less-exposed occupations. Regarding Agriculture, Forestry and Fishing, duration of exposure altered AST, ALP, and total bilirubin significantly (p < 0.05) while ALT and GGT were altered moderately significantly (p < 0.10). With mining, duration of exposure altered AST and GGT moderately significantly, whereas in construction duration in occupation altered AST, and GGT significantly, and total bilirubin moderately significantly. The study findings are evidence of occupational exposure to lead playing a significant role in initiating and promoting adverse hepatobiliary clinical outcomes in United States adults.
Evaluating the Multi-Functionality of Forest Ecosystems in Northern Mexico
Authors: Perez-Verdin Gustavo , Monarrez-Gonzalez Jose , Tecle Aregai , Pompa-Garcia Marin
Published: April 2018

Managing multiple ecosystem services is a complex task that involves special interactions among different resources, services, and stakeholders. Mexican forests have been traditionally managed for the single purpose of obtaining wood, benefiting a small sector of society. In this study, we evaluated the interactions among various ecosystem services, namely carbon content, tree diversity, surface water runoff, and the net present value of timber production. We also attempted to determine the most suitable basal area level that best satisfies the management of these services combined. Bivariate correlations, non-linear regression models, and a multiobjective decision-making technique are used to analyze the data in the study. Results indicate that trade-offs exist between surface water runoff and tree basal area. A synergistic relationship, between net present value and carbon content with basal area, was also observed. Tree diversity has a synergistic relationship with basal area in open forests, but a trade-off relationship in denser forests. The most preferred forest management level that satisfies the desired ecosystem services is between 17 and 21 m2/ha of residual basal area. We hope that adopting this multiobjective study can cement collaborative strategies among Mexican resource managers, landowners, environmental groups, and others interested in forest management.
Effect of Sludge Concentration and Crude Glycerol Matrix as a Substrate on the Production of Single-Cell Oil by Oleaginous Yeast Yarrowia lipolytica SKY7
Authors: Ram Saurabh , Tyagi Rajeshwar , Drogui Patrick
Published: April 2018

The disposal of excess crude glycerol produced by the booming biodiesel industry and wastewater sludge solid waste has become a severe problem, and alternate routes of use and valorization of these waste byproducts are needed. The use of cheaply available wastewater sludge solids in fermentation media is very much desirable to reduce the cost of production. The strains of Yarrowia lipolytica can assimilate a wide array of waste substrates, such as crude glycerol, waste cooking oil, starch wastewater, and cellulosic. This study optimized the concentration of wastewater sludge solids (5–35 g/L) to be used with crude glycerol in fermentation media to produce microbial oil as feedstock for biodiesel production. The results indicated that 20 g/L of sludge solids with 40 g/L of crude glycerol resulted in highest lipid content of 29.35% in 96 h. Further, assuming wet extraction of lipids, it was found that at least 11.2% or higher lipid content is required for this process to have an overall positive net solid waste reduction. Insignificant inhibition was observed by the crude glycerol used in this study as compared to pure glycerol, which proves it to be an adequate source of carbon substrate for lipid production.
Key Stages of Fiber Development as Determinants of Bast Fiber Yield and Quality
Authors: Mokshina Natalia , Chernova Tatyana , Galinousky Dmitry , Gorshkov Oleg , Gorshkova Tatyana
Published: April 2018

Plant fibers find wide application in various fields that demand specific parameters of fiber quality. To develop approaches for the improvement of yield and quality of bast fibers, the knowledge of the fiber developmental stages and of the key molecular players that are responsible for a certain parameter, are vitally important. In the present review the key stages of fiber development, such as initiation, intrusive growth, and formation of thickened cell wall layers (secondary and tertiary cell walls) are considered, as well as the impact of each of these stages on the final parameters of fiber yield and quality. The problems and perspectives of crop quality regulation are discussed.
Swedish Consumers’ Perception of Food Quality and Sustainability in Relation to Organic Food Production
Authors: Bosona Techane , Gebresenbet Girma
Published: April 2018

Consumers’ demand for locally produced and organic foods has increased in Sweden. This paper presents the results obtained from the analysis of data acquired from 100 consumers in Sweden who participated in an online survey during March to June 2016. The objective was to identify consumers’ demand in relation to organic food and sustainable food production, and to understand how the consumers evaluate food quality and make buying decisions. Qualitative descriptions, descriptive statistics and Pearson’s Chi-square test (with alpha value of p < 0.05 as level of significance), and Pearson’s correlation coefficient were used for analysis. About 72% of participants have the perception that organic food production method is more sustainable than conventional methods. Female consumers have more positive attitudes than men towards organic food. However, age difference, household size and income level do not significantly influence the consumers’ perception of sustainable food production concepts. Regionality, sustainable methods of production and organic production are the most important parameters to characterize the food as high quality and make buying decisions. On the other hand, product uniformity, appearance, and price were found to be relatively less important parameters. Food buying decisions and food quality were found to be highly related with Pearson’s correlation coefficient of r = 0.99.
Applying MOG to Lensing: Einstein Rings, Abell 520 and the Bullet Cluster
Authors: Moffat John , Rahvar Sohrab , Toth Viktor
Published: April 2018

We investigate gravitational lensing in the context of the MOG modified theory of gravity. Using a formulation of the theory with no adjustable or fitted parameters, we present the MOG equations of motion for slow, nonrelativistic test particles and for ultrarelativistic test particles, such as rays of light. We demonstrate how the MOG prediction for the bending of light can be applied to astronomical observations. Our investigation first focuses on a small set of strong lensing observations where the properties of the lensing objects are found to be consistent with the predictions of the theory. We also present an analysis of the colliding clusters 1E0657-558 (known also as the Bullet Cluster) and Abell 520; in both cases, the predictions of the MOG theory are in good agreement with observation.
Lithogenic and Anthropogenic Components in Surface Sediments from Lake Limboto as Shown by Magnetic Mineral Characteristics, Trace Metals, and REE Geochemistry
Authors: Yunginger Raghel , Bijaksana Satria , Dahrin Darharta , Zulaikah Siti , Hafidz Abd , Kirana Kartika , Sudarningsih Sudarningsih , Mariyanto Mariyanto , Fajar Silvia
Published: April 2018

Lake Limboto is one of the major lakes in Sulawesi, Indonesia. It is currently undergoing serious degradation due to population pressure. As more residential areas have been established around the lake, the sedimentation rate has increased because of the contribution of anthropogenic particles. In this study, the lithogenic and anthropogenic components in surface sediments from 17 points in the lake were studied and identified using a combination of magnetic and geochemical analyses. The results showed that although the magnetic susceptibility values in R (residential) and NR (non-residential) areas were relatively similar, the values of saturation isothermal remanent magnetization (SIRM) as well as those of SIRM/χLF differed significantly, implying that the magnetic characteristics of the lithogenic component (in the NR area) differ from those of the anthropogenic component (in the R area). The discrepancy between the anthropogenic and lithogenic contributions was further supported by trace metals and rare earth element (REE) contents. Sediment samples in the R area contained higher levels of Mn, La, Pr, and Gd, while in the NR area they contained higher levels of Fe, Sc, Nd, and Ce. The magnetic susceptibility also correlated strongly with Fe, Cu, Zn, and Mn contents in the NR area. A similar correlation was not observed in the R area. The results above imply that a combination of magnetic and geochemical analyses can successfully differentiate lithogenic and anthropogenic components or contributions in lake sediments.
Multiscale and Hierarchical Classification for Benthic Habitat Mapping
Authors: Porskamp Peter , Rattray Alex , Young Mary , Ierodiaconou Daniel
Published: April 2018

Developing quantitative and objective approaches to integrate multibeam echosounder (MBES) data with ground observations for predictive modelling is essential for ensuring repeatability and providing confidence measures for benthic habitat mapping. The scale of predictors within predictive models directly influences habitat distribution maps, therefore matching the scale of predictors to the scale of environmental drivers is key to improving model accuracy. This study uses a multi-scalar and hierarchical classification approach to improve the accuracy of benthic habitat maps. We used a 700-km2 region surrounding Cape Otway in Southeast Australia with full MBES data coverage to conduct this study. Additionally, over 180 linear kilometers of towed video data collected in this area were classified using a hierarchical classification approach. Using a machine learning approach, Random Forests, we combined MBES bathymetry, backscatter, towed video and wave exposure to model the distribution of biotic classes at three hierarchical levels. Confusion matrix results indicated that greater numbers of classes within the hierarchy led to lower model accuracy. Broader scale predictors were generally favored across all three hierarchical levels. This study demonstrates the benefits of testing predictor scales across multiple hierarchies for benthic habitat characterization.
Gleaning and Dreaming on Car Park Beach
Authors: Croft Jo
Published: April 2018

This article explores beachcombing and gleaning as practices that combine mobility with daydreaming and which allow us to experience our environment with the perception of ‘tactile nearness’ (Benjamin). Through eco-poetics shaped by ‘inconceivable analogies and connections’ (Benjamin), the author re-imagines a neglected space used as a short-cut on the way to work—the Liverpool Adelphi car park in Liverpool—as “Car Park Beach”. Inspired by the situationists’ slogan ‘Sous les pavés, la plage’, the author argues that Car Park Beach opens up imaginative possibilities for a different form of ecological encounter with our own precarity, one ushered in by a ‘close-up’ awareness of how waste transforms our world. Car Park Beach is a site that the author associates with the drift-like, distracted movements of both people and matter, and this article therefore attempts to deploy an equivalent method of analysis. Drawing on her own practice of gleaning photos and objects on the way to work, the author places a vocabulary of flotsam and jetsam at the axis of her discussion. Allusive, often layered, connections are followed between a diverse range of sources including beachcombing guides, literary memoirs, documentary films, eco-criticism, and auto-ethnography.
Cooking Fuels in Lagos, Nigeria: Factors Associated with Household Choice of Kerosene or Liquefied Petroleum Gas (LPG)
Authors: Ozoh Obianuju , Okwor Tochi , Adetona Olorunfemi , Akinkugbe Ayesha , Amadi Casmir , Esezobor Christopher , Adeyeye Olufunke , Ojo Oluwafemi , Nwude Vivian , Mortimer Kevin
Published: April 2018

Cooking with dirty-burning fuels is associated with health risk from household air pollution. We assessed the prevalence of and factors associated with the use of cooking fuels, and attitudes and barriers towards use of liquefied petroleum gas (LPG). This was a cross-sectional, population-based survey conducted in 519 households in Lagos, Nigeria. We used a structured questionnaire to obtain information regarding choice of household cooking fuel and the attitudes towards the use of LPG. Kerosene was the most frequently used cooking fuel (n = 475, 91.5%; primary use n = 364, 70.1%) followed by charcoal (n = 159, 30.6%; primary use n = 88, 17%) and LPG (n = 86, 16.6%; primary use n = 63, 12.1%). Higher level of education, higher income and younger age were associated with LPG vs. kerosene use. Fuel expenditure on LPG was significantly lower than for kerosene ( N (Naira) 2169.0 ± 1507.0 vs. N 2581.6 ± 1407.5). Over 90% of non-LPG users were willing to switch to LPG but cited safety issues and high cost as potential barriers to switching. Our findings suggest that misinformation and beliefs regarding benefits, safety and cost of LPG are important barriers to LPG use. An educational intervention program could be a cost-effective approach to improve LPG adoption and should be formally addressed through a well-designed community-based intervention study.
Determinants of Child Health Behaviors in a Disadvantaged Area from a Community Perspective: A Participatory Needs Assessment
Authors: Anselma Manou , Chinapaw Mai , Altenburg Teatske
Published: April 2018

Children from disadvantaged areas are hard to reach for interventions aimed at promoting healthy lifestyles. We conducted a participatory needs assessment, in which researchers collaborated with a community in a disadvantaged area in Amsterdam to gain an understanding of the health-related issues of children within this community. Qualitative data was collected through: three to four participatory group meetings with three groups of 9–12-year-old children (n = 5–9 per group); nine interviews with professionals working with youth; two interviews with parents and their children; and informal meetings including 31 parents. All transcriptions or summaries were coded and analyzed. Childhood overweight/obesity was indicated as the main health issue. A lack of physical activity and unhealthy dietary behavior were identified as the main risk factors, with underlying determinants such as culture, habits, finances, and social norms. Identified needs included more supervised, low-priced sports activities at a nearby location and more education on adopting a healthy diet. Our participatory health needs assessment resulted in a comprehensive overview of the most relevant risk factors and determinants of childhood overweight/obesity and needs from the community’s perspective. This knowledge aids in the development of better tailored, and thereby potentially more effective, interventions.
Factors that Predict Parental Willingness to Have Their Children Vaccinated against HPV in a Country with Low HPV Vaccination Coverage
Authors: Ganczak Maria , Owsianka Barbara , Korzeń Marcin
Published: April 2018

Background: Adolescent HPV (Human Papilloma Virus) vaccination is yet to be introduced as a mandatory program in Poland. Polish literature on factors associated with adolescent HPV vaccination is scant, despite the fact that uptake is one of the poorest in the European Union. Objectives: To assess HPV awareness and identify independent predictors for parental willingness to have their children vaccinated against HPV. Methods: All parents of first grade students from three selected high schools in Zgorzelec, Poland, who participated in parent–teacher meetings at the time the study was conducted, had their children unvaccinated regarding HPV, and who gave informed consent to participate were included. There were 600 first grade students; 9 were vaccinated against HPV. This left 591 parents who met the eligibility criteria; the response rate was 76.1%. Results: Awareness of HPV was reported by 55.3% of 450 parents (mean age 42 years, 70.9% females); 85.1% expressed their willingness to vaccinate their children against HPV; 31.3% identified HPV as a sexually transmitted pathogen, and 36.2% identified it as a risk factor of cervical cancer. Multivariable logistic regression analyses indicated that being employed (OR 2.09; 95% CI: 1.10–3.86), having positive attitudes toward vaccines (OR 3.02; 95% CI: 1.34–6.49), previous information about HPV (OR 2.02; 95% CI: 1.17–3.51), and concerns about the side effects of the HPV vaccine (OR 0.60; 95% CI: 0.35–0.99) were independent predictors of parents’ willingness to vaccinate. Conclusions: Attitudes regarding their child being vaccinated against HPV were positive among Polish parents, even though awareness and knowledge of HPV in this group were low. Most of the significant factors that influenced their willingness were modifiable, such as being informed about HPV and having positive attitudes toward vaccines. Future interventions should focus specifically on vulnerable subgroups, such as unemployed parents.
Influence of Vehicle Speed on the Characteristics of Driver’s Eye Movement at a Highway Tunnel Entrance during Day and Night Conditions: A Pilot Study
Authors: Qin Li , Dong Li-Li , Xu Wen-Hai , Zhang Li-Dong , Leon Arturo
Published: April 2018

The aim of this study was to investigate how vehicle speed influences the characteristics of driver’s eye movement at highway tunnel entrances during day and night. In this study, six drivers’ eye movement data (from 200 m before tunnel entrance to 200 m inside tunnel entrance) under five predetermined vehicle speeds (40, 50, 60, 70 and 80 km/h) in the daytime and three predetermined vehicle speeds (40, 60 and 80 km/h) in the nighttime were recorded using the non-intrusive Dikablis Professional eye-tracking system. Pupil size, the average fixation duration time and the average number of fixation were analyzed and then the influence of the vehicle speed on these parameters was evaluated by means of IBM SPSS Statistics 20.0. The results for pupil size in daytime increased when approaching the tunnel entrance, while as for nighttime, pupil size decreased when approaching the tunnel entrance and then increased after entering the tunnel. The pupil size in daytime has a significant negative correlation with vehicle speed, while the pupil size in nighttime did not show a significant association with vehicle speed. Furthermore, the average fixation duration in daytime increased when entering the tunnel, and had a significant negative correlation with vehicle speed. Also, the average number of fixations in daytime decreased when entering the tunnel and has a significant negative correlation with vehicle speed. However, the average fixation duration and the average number of fixations in nighttime did not show any significant association with vehicle speed. Moreover, limitations and future directions of the study are discussed for the further investigation.
Why Are New Tobacco Control Interventions Needed?
Authors: Nagelhout Gera , Popova Lucy , Kuipers Mirte
Published: April 2018

It has been known for years which policies and interventions work to decrease tobacco use in the population[...]
Medical Surveillance, Continuous Health Promotion and a Participatory Intervention in a Small Company
Authors: Magnavita Nicola
Published: April 2018

The workplace is an ideal setting for health promotion. The regular medical examination of workers enables us to screen for numerous diseases, spread good practices and correct lifestyles, and obtain a favourable risk/benefit ratio. The continuous monitoring of the level of workers’ wellbeing using a holistic approach during medical surveillance enables us to promptly identify problems in work organisation and the company climate. Problems of this kind can be adequately managed by using a participatory approach. The aim of this paper is twofold: to signal this way of proceeding with medical surveillance, and to describe an organisational development intervention. Participatory groups were used to improve occupational life in a small company. After intervention we observed a reduction in levels of perceived occupational stress measured with the Effort/Reward Imbalance questionnaire, and an improvement in psychological wellbeing assessed by means of the Goldberg Anxiety/Depression scale. Although the limited size of the sample and the lack of a control group call for a cautious evaluation of this study, the participatory strategy proved to be a useful tool due to its cost-effectiveness.
A Novel Approach for Identifying Urban Built-Up Area Boundaries Using High-Resolution Remote-Sensing Data Based on the Scale Effect
Authors: Zhou Yi , Tu Mingguang , Wang Shixin , Liu Wenliang
Published: April 2018

Identifying urban built-up area boundaries is critical to urban data statistics, size measurement, and spatial control. However, previous methods of extracting urban built-up area boundaries based on low-resolution remote-sensing data are frequently constrained by data accuracy. In this paper, a new method for extracting urban built-up area boundaries using high-resolution remote sensing images based on scale effects is proposed. Firstly, we generate a number of different levels of edge-multiplied hexagonal vector grids. Secondly, the impervious surface densities are calculated based on the hexagonal vector grids with the longest edge. Then, the hexagonal grids with higher impervious surface densities are extracted as the built-up area of the first level. Thirdly, we gradually reduce the spatial scale of the hexagonal vector grid and repeat the extraction process based on the extracted built-up area in the previous step. Eventually, we obtain the urban built-up area boundary at the smallest scale. Plausibility checks indicate that the suggested method not only guarantees the spatial continuity of the resultant urban built-up area boundary, but also highlights the prevailing orientation of urban expansion. The extracted Beijing built-up area boundary can serve as a reference in decision-making for space planning and land-use control.
Feature Tracking for High Speed AFM Imaging of Biopolymers
Authors: Hartman Brett , Andersson Sean
Published: April 2018

The scanning speed of atomic force microscopes continues to advance with some current commercial microscopes achieving on the order of one frame per second and at least one reaching 10 frames per second. Despite the success of these instruments, even higher frame rates are needed with scan ranges larger than are currently achievable. Moreover, there is a significant installed base of slower instruments that would benefit from algorithmic approaches to increasing their frame rate without requiring significant hardware modifications. In this paper, we present an experimental demonstration of high speed scanning on an existing, non-high speed instrument, through the use of a feedback-based, feature-tracking algorithm that reduces imaging time by focusing on features of interest to reduce the total imaging area. Experiments on both circular and square gratings, as well as silicon steps and DNA strands show a reduction in imaging time by a factor of 3–12 over raster scanning, depending on the parameters chosen.
Mixture Concentration-Response Modeling Reveals Antagonistic Effects of Estradiol and Genistein in Combination on Brain Aromatase Gene (cyp19a1b) in Zebrafish
Authors: Hinfray Nathalie , Tebby Cleo , Piccini Benjamin , Bourgine Gaelle , Aït-Aïssa Sélim , Porcher Jean-Marc , Pakdel Farzad , Brion François
Published: April 2018

Comprehension of compound interactions in mixtures is of increasing interest to scientists, especially from a perspective of mixture risk assessment. However, most of conducted studies have been dedicated to the effects on gonads, while only few of them were. interested in the effects on the central nervous system which is a known target for estrogenic compounds. In the present study, the effects of estradiol (E2), a natural estrogen, and genistein (GEN), a phyto-estrogen, on the brain ER-regulated cyp19a1b gene in radial glial cells were investigated alone and in mixtures. For that, zebrafish-specific in vitro and in vivo bioassays were used. In U251-MG transactivation assays, E2 and GEN produced antagonistic effects at low mixture concentrations. In the cyp19a1b-GFP transgenic zebrafish, this antagonism was observed at all ratios and all concentrations of mixtures, confirming the in vitro effects. In the present study, we confirm (i) that our in vitro and in vivo biological models are valuable complementary tools to assess the estrogenic potency of chemicals both alone and in mixtures; (ii) the usefulness of the ray design approach combined with the concentration-addition modeling to highlight interactions between mixture components.
Potential Interplay between Hyperosmolarity and Inflammation on Retinal Pigmented Epithelium in Pathogenesis of Diabetic Retinopathy
Authors: Willermain François , Scifo Lisa , Weber Célia , Caspers Laure , Perret Jason , Delporte Christine
Published: April 2018

Diabetic retinopathy is a frequent eyesight threatening complication of type 1 and type 2 diabetes. Under physiological conditions, the inner and the outer blood-retinal barriers protect the retina by regulating ion, protein, and water flux into and out of the retina. During diabetic retinopathy, many factors, including inflammation, contribute to the rupture of the inner and/or the outer blood-retinal barrier. This rupture leads the development of macular edema, a foremost cause of sight loss among diabetic patients. Under these conditions, it has been speculated that retinal pigmented epithelial cells, that constitute the outer blood-retinal barrier, may be subjected to hyperosmolar stress resulting from different mechanisms. Herein, we review the possible origins and consequences of hyperosmolar stress on retinal pigmented epithelial cells during diabetic retinopathy, with a special focus on the intimate interplay between inflammation and hyperosmolar stress, as well as the current and forthcoming new pharmacotherapies for the treatment of such condition.
Ixeris dentata Extract Increases Salivary Secretion through the Regulation of Endoplasmic Reticulum Stress in a Diabetes-Induced Xerostomia Rat Model
Authors: Bhattarai Kashi , Lee Hwa-Young , Kim Seung-Hyun , Kim Hyung-Ryong , Chae Han-Jung
Published: April 2018

This study aimed to investigate the molecular mechanism of diabetes mellitus (DM)-induced dry mouth and an application of natural products from Ixeris dentata (IXD), a recently suggested regulator of amylase secretion in salivary cells. Vehicle-treated or diabetic rats were orally treated with either water or an IXD extract for 10 days to observe the effect on salivary flow. We found that the IXD extract increased aquaporin 5 (AQP5) and alpha-amylase protein expression in the submandibular gland along with salivary flow rate. Similarly, the IXD extract and its purified compound increased amylase secretion in high glucose-exposed human salivary gland cells. Furthermore, increased endoplasmic reticulum stress response in the submandibular gland of diabetic rats was inhibited by treatment with the IXD extract, suggesting that IXD extract treatment improves the ER environment by increasing the protein folding capacity. Thus, pharmacological treatment with the IXD extract is suggested to relieve DM-induced dry mouth symptoms.
Rhein Induces Cell Death in HepaRG Cells through Cell Cycle Arrest and Apoptotic Pathway
Authors: You Longtai , Dong Xiaoxv , Yin Xingbin , Yang Chunjing , Leng Xin , Wang Wenping , Ni Jian
Published: April 2018

Rhein, a naturally occurring active anthraquinone found abundantly in various medicinal and nutritional herbs, possesses a wide spectrum of pharmacological effects. Furthermore, previous studies have reported that rhein could induce hepatotoxicity in rats. However, its cytotoxicity and potential molecular mechanisms remain unclear. Therefore, the present study aimed to investigate the cytotoxicity of rhein on HepaRG cells and the underlying mechanisms of its cytotoxicity. Our results demonstrate, by 3-(4,5-dimethyl thiazol-2-yl-)-2,5-diphenyl tetrazolium bromide (MTT) and Annexin V-fluoresce isothiocyanate (FITC)/propidium iodide (PI) double-staining assays, that rhein significantly inhibited cell viability and induced apoptosis in HepaRG cells. Moreover, rhein treatment resulted in the generation of reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP), and S phase cell cycle arrest. The results of Western blotting showed that rhein treatment resulted in a significant increase in the protein levels of Fas, p53, p21, Bax, cleaved caspases-3, -8, -9, and poly(ADP-ribose)polymerase (PARP). The protein expression of Bcl-2, cyclin A, and cyclin-dependent kinase 2 (CDK 2) was decreased. In conclusion, these results suggest that rhein treatment could inhibit cell viability of HepaRG cells and induce cell death through cell cycle arrest in the S phase and activation of Fas- and mitochondrial-mediated pathways of apoptosis. These findings emphasize the need to assess the risk of exposure for humans to rhein.
Kv3.1 and Kv3.4, Are Involved in Cancer Cell Migration and Invasion
Authors: Song Min , Park Su , Park Jeong , Byun Jin , Jin Hee , Seo Seung , Ryu Pan , Lee So
Published: April 2018

Voltage-gated potassium (Kv) channels, including Kv3.1 and Kv3.4, are known as oxygen sensors, and their function in hypoxia has been well investigated. However, the relationship between Kv channels and tumor hypoxia has yet to be investigated. This study demonstrates that Kv3.1 and Kv3.4 are tumor hypoxia-related Kv channels involved in cancer cell migration and invasion. Kv3.1 and Kv3.4 protein expression in A549 and MDA-MB-231 cells increased in a cell density-dependent manner, and the pattern was similar to the expression patterns of hypoxia-inducible factor-1α (HIF-1α) and reactive oxygen species (ROS) according to cell density, whereas Kv3.3 protein expression did not change in A549 cells with an increase in cell density. The Kv3.1 and Kv3.4 blocker blood depressing substance (BDS) did not affect cell proliferation; instead, BDS inhibited cell migration and invasion. We found that BDS inhibited intracellular pH regulation and extracellular signal-regulated kinase (ERK) activation in A549 cells cultured at a high density, potentially resulting in BDS-induced inhibition of cell migration and invasion. Our data suggest that Kv3.1 and Kv3.4 might be new therapeutic targets for cancer metastasis.
CRISPR/Cas9-Mediated Mutagenesis of Carotenoid Cleavage Dioxygenase 8 (CCD8) in Tobacco Affects Shoot and Root Architecture
Authors: Gao Junping , Zhang Tong , Xu Bingxin , Jia Ling , Xiao Bingguang , Liu He , Liu Lijing , Yan Hao , Xia Qingyou
Published: April 2018

Strigolactones (SLs) are a class of phytohormones that regulate plant architecture. Carotenoid cleavage dioxygenase (CCD) genes are involved in the biosynthesis of SLs and are identified and characterized in many plants. However, the function of CCD genes in tobacco remains poorly understood. In this study, two closely related genes NtCCD8A and NtCCD8B were cloned from tobacco (Nicotiana tabacum L.). The two NtCCD8 genes are orthologues of the tomato (Solanum lycopersicum) carotenoid cleavage dioxygenase 8 (SlCCD8) gene. NtCCD8A and NtCCD8B were primarily expressed in tobacco roots, but low expression levels of these genes were detected in all plant tissues, and their transcript levels significantly increased in response to phosphate limitation. NtCCD8A and NtCCD8B mutations were introduced into tobacco using the CRISPR/Cas9 system and transgenic tobacco lines for both ntccd8 mutant alleles were identified. The ntccd8a and ntccd8b mutant alleles were inactivated by a deletion of three nucleotides and insertion of one nucleotide, respectively, both of which led to the production of premature stop codons. The ntccd8 mutants had increased shoot branching, reduced plant height, increased number of leaves and nodes, and reduced total plant biomass compared to wild-type plants; however, the root-to-shoot ratio was unchanged. In addition, mutant lines had shorter primary roots and more of lateral roots than wild type. These results suggest that NtCCD8 genes are important for changes in tobacco plant architecture.
Impact of Subolesin and Cystatin Knockdown by RNA Interference in Adult Female Haemaphysalis longicornis (Acari: Ixodidae) on Blood Engorgement and Reproduction
Authors: Rahman Md. , Saiful Islam Mohammad , You Myungjo
Published: April 2018

Currently, multi-antigenic vaccine use is the method of choice for the strategic control of ticks. Therefore, determining the efficacy of combined antigens is a promising avenue of research in the development of anti-tick vaccines. The antigen responsible for blood intake and reproduction has proven suitable as a vaccine antigen. It has been shown to silence Haemaphysalis longicornis salivary cystatin (HlSC-1) and subolesin by RNA interference. Adult unfed female ticks were injected with double-stranded RNA of (A) subolesin, (B) cystatin, (C) subolesin plus cystatin, and (D) injection buffer, then fed alongside normal unfed males up to spontaneous drop-down. The percentage of knockdowns was determined by real-time polymerase chain reaction. Sixty-three percent and 53% knockdown rates were observed in subolesin and cystatin double-stranded RNA-injected ticks respectively, while 32 and 26% knockdown rates of subolesin and cystatin transcript were observed in subolesin plus cystatin double-stranded RNA-injected ticks. Subolesin and/or cystatin knockdown causes a significant (p < 0.05) reduction in tick engorgement, egg mass weight, and egg conversion ratio. Most importantly, combined silencing did not act synergistically, but caused a similarly significant (p < 0.05) reduction in tick engorgement, egg mass weight, and egg conversion ratio. Therefore, the elucidation of multiple antigens may be helpful in the future of vaccines.
Role of Stromal Paracrine Signals in Proliferative Diseases of the Aging Human Prostate
Authors: Ishii Kenichiro , Takahashi Sanai , Sugimura Yoshiki , Watanabe Masatoshi
Published: April 2018

Androgens are essential for the development, differentiation, growth, and function of the prostate through epithelial–stromal interactions. However, androgen concentrations in the hypertrophic human prostate decrease significantly with age, suggesting an inverse correlation between androgen levels and proliferative diseases of the aging prostate. In elderly males, age- and/or androgen-related stromal remodeling is spontaneously induced, i.e., increased fibroblast and myofibroblast numbers, but decreased smooth muscle cell numbers in the prostatic stroma. These fibroblasts produce not only growth factors, cytokines, and extracellular matrix proteins, but also microRNAs as stromal paracrine signals that stimulate prostate epithelial cell proliferation. Surgical or chemical castration is the standard systemic therapy for patients with advanced prostate cancer. Androgen deprivation therapy induces temporary remission, but the majority of patients eventually progress to castration-resistant prostate cancer, which is associated with a high mortality rate. Androgen deprivation therapy-induced stromal remodeling may be involved in the development and progression of castration-resistant prostate cancer. In the tumor microenvironment, activated fibroblasts stimulating prostate cancer cell proliferation are called carcinoma-associated fibroblasts. In this review, we summarize the role of stromal paracrine signals in proliferative diseases of the aging human prostate and discuss the potential clinical applications of carcinoma-associated fibroblast-derived exosomal microRNAs as promising biomarkers.
Restrictions in the Ankle Sagittal- and Frontal-Plane Range of Movement during Simulated Walking with Different Types of Orthoses
Authors: Thiele Frank , Schuhmacher Sebastian , Schwaller Christoph , Plüss Stefan , Rhiner Joël , List Renate , Lorenzetti Silvio
Published: April 2018

Different types of orthoses are available to clinicians for non-surgical treatment of acute ankle sprains. The goal of this study was to scientifically compare the movement restrictions in the sagittal and frontal plane during simulated walking between one adaptable semi-rigid brace (OrthoTri-PhaseTM), four non-adaptable semi-rigid braces (OrthoStandardTM, MalleoLocTM, MalleoSprintTM, VACOankleTM), and one rigid cast. Predefined time sequences of rotational moments and axial loading during gait were applied via an ankle joint simulator, with the pneumatic pressure inside the orthoses kept constant to ensure the same condition for different trials and orthoses. The peak ranges of motion (RoMs) in the frontal and sagittal plane during gait were analyzed for statistically significant differences using single-factorial ANOVA with post-hoc Bonferroni analysis. Significant differences in peak plantar-/dorsiflexion and in-/eversion RoM during gait were found between different types of orthoses. In the sagittal plane, the rigid cast most significantly restricted overall RoM followed by the Ortho Tri-PhaseTM in Phase 1 and the Ortho StandardTM. The peak restriction in-/eversion RoM of the VACOankleTM came closest to the rigid cast, with a shift towards inversion. The VACOankleTM allowed for significantly larger dorsiflexion movement compared to all other orthoses. The present results may help clinicians in the decision-making process of finding the optimal orthosis for individual patients.
The Implications of Oil Exploration off the Gulf Coast of Florida
Authors: Nelson Jake , Grubesic Tony
Published: April 2018

In the United States (U.S.), oil exploration and production remain critical economic engines for local, state, and federal economies. Recently, the U.S. Department of the Interior expressed interest in expanding offshore oil production by making available lease areas in the U.S. Gulf of Mexico, the U.S. West Coast and East Coast, as well as offshore Alaska. With the promise of aiding in energy independence, these new lease areas could help solidify the U.S. as one of the world’s largest oil-producing countries, while at the same time bolstering the local and regional energy job sectors. Of all the newly proposed lease areas, the Gulf Coast of Florida is particularly contentious. Opponents of drilling in the area cite the sensitive ecosystems and the local and state tourism economy that depends heavily on the numerous beaches lining Florida’s coast. In this analysis, we use a data-driven spatial analytic approach combined with advanced oil spill modeling to determine the potential impact of oil exploration off of Florida’s Gulf Coast given a loss-of-control event. It is determined that plume behavior varies drastically depending on the location of the spill but that overall impacts are comparable across all spill scenario sites, highlighting the necessity of contingency-type analyses. Implications for spill response are also discussed.
Design of a Broadband Tunable Terahertz Metamaterial Absorber Based on Complementary Structural Graphene
Authors: Huang Mu , Cheng Yong , Cheng Zheng , Chen Hao , Mao Xue , Gong Rong
Published: April 2018

We present a simple design for a broadband tunable terahertz (THz) metamaterial absorber (MMA) consisting of a complementary cross-oval-shaped graphene (CCOSG) structure and dielectric substrate placed on a continuous metal film. Both numerical simulation and theoretical calculation results indicate that the absorbance is greater than 80% from 1.2 to 1.8 THz, and the corresponding relative bandwidth is up to 40%. Simulated electric field and power loss density distributions reveal that the broadband absorption mainly originates from the excitation of continuous surface plasmon resonance (SPR) on the CCOSG. In addition, the MMA is polarization-insensitive for both transverse-electric (TE) and transverse-magnetic (TM) modes due to the geometry rotational symmetry of the unit-cell structure. Furthermore, the broadband absorption properties of the designed MMA can be effectively tunable by varying the geometric parameters of the unit-cell and chemical potential of graphene. Our results may find promising applications in sensing, detecting, and optoelectronic-related devices.
Effects of Setting Errors (Insert Run-Outs) on Surface Roughness in Face Milling When Using Circular Inserts
Authors: Felhő Csaba , Kundrák János
Published: April 2018

In face milling, the roughness of the machined surface varies due to the movement of the cutting edge. Changes in roughness parameter values in the axis of rotation (symmetry plane) have been examined at a constant depth of cut for symmetrical milling. In this paper, the effect of increasing feed per tooth on the topography of the surface is studied in fly-cutting and in multi-point face milling. The study takes into account the axial run-out of the inserts. Theoretical roughness values were modelled, the real values were tested in experiments and in both cases the impact of the run-out of the cutting edges and the change of the chip cross-section were also taken into account. Based on the performed experiments it can be stated that the accuracy of the introduced roughness prediction method increases with the increase in feed and therefore the application of the method in the case of high-feed milling is particularly effective. The results have also shown that the run-out of the insert significantly effects the roughness of the milled surfaces and therefore the measurement and minimization of these setting errors is essential.
A Machine Learning Approach to Determine Oyster Vessel Behavior
Authors: Frey Devin Joseph , Mishra Avdesh , Hoque Md Tamjidul , Abdelguerfi Mahdi , Soniat Thomas
Published: April 2018

In this work, we address a multi-class classification task of oyster vessel behaviors determination by classifying them into four different classes: fishing, traveling, poling (exploring) and docked (anchored). The main purpose of this work is to automate the oyster vessel behaviors determination task using machine learning and to explore different techniques to improve the accuracy of the oyster vessel behavior prediction problem. To employ machine learning technique, two important descriptors: speed and net speed, are calculated from the trajectory data, recorded by a satellite communication system (Vessel Management System, VMS) attached to the vessels fishing on the public oyster grounds of Louisiana. We constructed a support vector machine (SVM) based method which employs Radial Basis Function (RBF) as a kernel to accurately predict the behavior of oyster vessels. Several validation and parameter optimization techniques were used to improve the accuracy of the SVM classifier. A total 93% of the trajectory data from a July 2013 to August 2014 dataset consisting of 612,700 samples for which the ground truth can be obtained using rule-based classifier is used for validation and independent testing of our method. The results show that the proposed SVM based method is able to correctly classify 99.99% of 612,700 samples using the 10-fold cross validation. Furthermore, we achieved a precision of 1.00, recall of 1.00, F1-score of 1.00 and a test accuracy of 99.99%, while performing an independent test using a subset of 93% of the dataset, which consists of 31,418 points.
Cloning, Synthesis and Functional Characterization of a Novel α-Conotoxin Lt1.3
Authors: Chen Jinqin , Liang Li , Ning Huying , Cai Fengtao , Liu Zhuguo , Zhang Longxiao , Zhou Liangyi , Dai Qiuyun
Published: April 2018

α-Conotoxins (α-CTxs) are small peptides composed of 11 to 20 amino acid residues with two disulfide bridges. Most of them potently and selectively target nicotinic acetylcholine receptor (nAChR) subtypes, and a few were found to inhibit the GABAB receptor (GABABR)-coupled N-type calcium channels (Cav2.2). However, in all of α-CTxs targeting both receptors, the disulfide connectivity arrangement “C1-C3, C2-C4” is present. In this work, a novel α4/7-CTx named Lt1.3 (GCCSHPACSGNNPYFC-NH2) was cloned from the venom ducts of Conus litteratus (C. litteratus) in the South China Sea. Lt1.3 was then chemically synthesized and two isomers with disulfide bridges “C1-C3, C2-C4” and “C1-C4, C2-C3” were found and functionally characterized. Electrophysiological experiments showed that Lt1.3 containing the common disulfide bridges “C1-C3, C2-C4” potently and selectively inhibited α3β2 nAChRs and not GABABR-coupled Cav2.2. Surprisingly, but the isomer with the disulfide bridges “C1-C4, C2-C3” showed exactly the opposite inhibitory activity, inhibiting only GABABR-coupled Cav2.2 and not α3β2 nAChRs. These findings expand the knowledge of the targets and selectivity of α-CTxs and provide a new structural motif to inhibit the GABABR-coupled Cav2.2.
Total Synthesis of Bioactive Marine Meroterpenoids: The Cases of Liphagal and Frondosin B
Authors: Zong Yan , Wang Weijia , Xu Tao
Published: April 2018

Liphagal and frondosin B are two marine-derived secondary metabolites sharing a very similar polyfused-benzofuran skeleton. The two tetracyclic meroterpenoids were isolated from marine sponges, both featuring a 6-5-7-6 fused ring system. A preliminary bioactive study shows that (+)-liphagal is a selective kinase (PI3K α) inhibitor, while (+)-frondosin B is shown to inhibit the binding of the cytokine interleukin-8 (IL-8) to its receptor, CX-CLR1/2. The unique structures and interesting biological profiles of these two meroterpenoids have attracted considerable attention from synthetic chemists. Herein we summarize the synthetic efforts with respect to (+)-liphagal and (+)-frondosin B during the past two decades.