bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function
Authors: Oliva María Ángela , Tosat-Bitrián Carlota , Barrado-Gil Lucía , Bonato Francesca , Galindo Inmaculada , Garaigorta Urtzi , Álvarez-Bernad Beatriz , París-Ogáyar Rebeca , Lucena-Agell Daniel , Giménez-Abián Juan Francisco , García-Dorival Isabel , Urquiza Jesús , Gastaminza Pablo , Díaz José Fernando , Palomo Valle , Alonso Covadonga
Published: March 2022

Microtubule targeting agents (MTAs) have been exploited mainly as anti-cancer drugs because of their impact on cellular division and angiogenesis. Additionally, microtubules (MTs) are key structures for intracellular transport, which is frequently hijacked during viral infection. We have analyzed the antiviral activity of clinically used MTAs in the infection of DNA and RNA viruses, including SARS-CoV-2, to find that MT destabilizer agents show a higher impact than stabilizers in the viral infections tested, and FDA-approved anti-helminthic benzimidazoles were among the most active compounds. In order to understand the reasons for the observed antiviral activity, we studied the impact of these compounds in motor proteins-mediated intracellular transport. To do so, we used labeled peptide tools, finding that clinically available MTAs impaired the movement linked to MT motors in living cells. However, their effect on viral infection lacked a clear correlation to their effect in motor-mediated transport, denoting the complex use of the cytoskeleton by viruses. Finally, we further delved into the molecular mechanism of action of Mebendazole by combining biochemical and structural studies to obtain crystallographic high-resolution information of the Mebendazole-tubulin complex, which provided insights into the mechanisms of differential toxicity between helminths and mammalians.
Diagnostic and Therapeutic Potential for HNP-1, HBD-1 and HBD-4 in Pregnant Women with COVID-19
Authors: Brancaccio Mariarita , Mennitti Cristina , Calvanese Mariella , Gentile Alessandro , Musto Roberta , Gaudiello Giulia , Scamardella Giulia , Terracciano Daniela , Frisso Giulia , Pero Raffaela , Sarno Laura , Guida Maurizio , Scudiero Olga
Published: March 2022

Pregnancy is characterized by significant immunological changes and a cytokine profile, as well as vitamin deficiencies that can cause problems for the correct development of a fetus. Defensins are small antimicrobial peptides that are part of the innate immune system and are involved in several biological activities. Following that, this study aims to compare the levels of various cytokines and to investigate the role of defensins between pregnant women with confirmed COVID-19 infection and pregnant women without any defined risk factor. TNF-α, TGF-β, IL-2 and IL-10, β-defensins, have been evaluated by gene expression in our population. At the same time, by ELISA assay IL-6, IL-8, defensin alpha 1, defensin beta 1 and defensin beta 4 have been measured. The data obtained show that mothers affected by COVID-19 have an increase in pro-inflammatory factors (TNF-α, TGF-β, IL-2, IL-6, IL-8) compared to controls; this increase could generate a sort of “protection of the fetus” from virus attacks. Contemporarily, we have an increase in the anti-inflammatory cytokine IL-10 and an increase in AMPs, which highlights how the mother’s body is responding to the viral attack. These results allow us to hypothesize a mechanism of “trafficking” of antimicrobial peptides from the mother to the fetus that would help the fetus to protect itself from the infection in progress.
Phasic Dopamine Changes and Hebbian Mechanisms during Probabilistic Reversal Learning in Striatal Circuits: A Computational Study
Authors: Schirru Miriam , Véronneau-Veilleux Florence , Nekka Fahima , Ursino Mauro
Published: March 2022

Cognitive flexibility is essential to modify our behavior in a non-stationary environment and is often explored by reversal learning tasks. The basal ganglia (BG) dopaminergic system, under a top-down control of the pre-frontal cortex, is known to be involved in flexible action selection through reinforcement learning. However, how adaptive dopamine changes regulate this process and learning mechanisms for training the striatal synapses remain open questions. The current study uses a neurocomputational model of the BG, based on dopamine-dependent direct (Go) and indirect (NoGo) pathways, to investigate reinforcement learning in a probabilistic environment through a task that associates different stimuli to different actions. Here, we investigated: the efficacy of several versions of the Hebb rule, based on covariance between pre- and post-synaptic neurons, as well as the required control in phasic dopamine changes crucial to achieving a proper reversal learning. Furthermore, an original mechanism for modulating the phasic dopamine changes is proposed, assuming that the expected reward probability is coded by the activity of the winner Go neuron before a reward/punishment takes place. Simulations show that this original formulation for an automatic phasic dopamine control allows the achievement of a good flexible reversal even in difficult conditions. The current outcomes may contribute to understanding the mechanisms for active control of dopamine changes during flexible behavior. In perspective, it may be applied in neuropsychiatric or neurological disorders, such as Parkinson’s or schizophrenia, in which reinforcement learning is impaired.
Dual Role of YY1 in HPV Life Cycle and Cervical Cancer Development
Authors: Warowicka Alicja , Broniarczyk Justyna , Węglewska Martyna , Kwaśniewski Wojciech , Goździcka-Józefiak Anna
Published: March 2022

Human papillomaviruses (HPVs) are considered to be key etiological agents responsible for the induction and development of cervical cancer. However, it has been suggested that HPV infection alone may not be sufficient to promote cervical carcinogenesis, and other unknown factors might be required to establish the disease. One of the suggested proteins whose deregulation has been linked with oncogenesis is transcription factor Yin Yang 1 (YY1). YY1 is a multifunctional protein that is involved not only in the regulation of gene transcription and protein modification, but can also control important cell signaling pathways, such as cell growth, development, differentiation, and apoptosis. Vital functions of YY1 also indicate that the protein could be involved in tumorigenesis. The overexpression of this protein has been observed in different tumors, and its level has been correlated with poor prognoses of many types of cancers. YY1 can also regulate the transcription of viral genes. It has been documented that YY1 can bind to the HPV long control region and regulate the expression of viral oncogenes E6 and E7; however, its role in the HPV life cycle and cervical cancer development is different. In this review, we explore the role of YY1 in regulating the expression of cellular and viral genes and subsequently investigate how these changes inadvertently contribute toward the development of cervical malignancy.
Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis
Authors: Lax Carlos , Cánovas-Márquez José Tomás , Tahiri Ghizlane , Navarro Eusebio , Garre Victoriano , Nicolás Francisco Esteban
Published: March 2022

The study of the Mucoralean fungi physiology is a neglected field that the lack of effective genetic tools has hampered in the past. However, the emerging fungal infection caused by these fungi, known as mucormycosis, has prompted many researchers to study the pathogenic potential of Mucorales. The main reasons for this current attraction to study mucormycosis are its high lethality, the lack of effective antifungal drugs, and its recent increased incidence. The most contemporary example of the emergence character of mucormycosis is the epidemics declared in several Asian countries as a direct consequence of the COVID-19 pandemic. Fortunately, this pressure to understand mucormycosis and develop new treatment strategies has encouraged the blossoming of new genetic techniques and methodologies. This review describes the history of genetic manipulation in Mucorales, highlighting the development of methods and how they allowed the main genetic studies in these fungi. Moreover, we have emphasized the recent development of new genetic models to study mucormycosis, a landmark in the field that will configure future research related to this disease.
Repurposing Dipyridamole in Niemann Pick Type C Disease: A Proof of Concept Study
Authors: Pepponi Rita , De Simone Roberta , De Nuccio Chiara , Visentin Sergio , Matteucci Andrea , Bernardo Antonietta , Popoli Patrizia , Ferrante Antonella
Published: March 2022

Niemann Pick type C disease (NPC) is a rare disorder characterized by lysosomal lipid accumulation that damages peripheral organs and the central nervous system. Currently, only miglustat is authorized for NPC treatment in Europe, and thus the identification of new therapies is necessary. The hypothesis addressed in this study is that increasing adenosine levels may represent a new therapeutic approach for NPC. In fact, a reduced level of adenosine has been shown in the brain of animal models of NPC; moreover, the compound T1-11, which is able to weakly stimulate A2A receptor and to increase adenosine levels by blocking the equilibrative nucleoside transporter ENT1, significantly ameliorated the pathological phenotype and extended the survival in a mouse model of the disease. To test our hypothesis, fibroblasts from NPC1 patients were treated with dipyridamole, a clinically-approved drug with inhibitory activity towards ENT1. Dipyridamole significantly reduced cholesterol accumulation in fibroblasts and rescued mitochondrial deficits; the mechanism elicited by dipyridamole relies on activation of the adenosine A2AR subtype subsequent to the increased levels of extracellular adenosine due to the inhibition of ENT1. In conclusion, our results provide the proof of concept that targeting adenosine tone could be beneficial in NPC.
Cav3 T-Type Voltage-Gated Ca2+ Channels and the Amyloidogenic Environment: Pathophysiology and Implications on Pharmacotherapy and Pharmacovigilance
Authors: Papazoglou Anna , Arshaad Muhammad Imran , Henseler Christina , Daubner Johanna , Broich Karl , Hescheler Jürgen , Ehninger Dan , Haenisch Britta , Weiergräber Marco
Published: March 2022

Voltage-gated Ca2+ channels (VGCCs) were reported to play a crucial role in neurotransmitter release, dendritic resonance phenomena and integration, and the regulation of gene expression. In the septohippocampal system, high- and low-voltage-activated (HVA, LVA) Ca2+ channels were shown to be involved in theta genesis, learning, and memory processes. In particular, HVA Cav2.3 R-type and LVA Cav3 T-type Ca2+ channels are expressed in the medial septum-diagonal band of Broca (MS-DBB), hippocampal interneurons, and pyramidal cells, and ablation of both channels was proven to severely modulate theta activity. Importantly, Cav3 Ca2+ channels contribute to rebound burst firing in septal interneurons. Consequently, functional impairment of T-type Ca2+ channels, e.g., in null mutant mouse models, caused tonic disinhibition of the septohippocampal pathway and subsequent enhancement of hippocampal theta activity. In addition, impairment of GABA A/B receptor transcription, trafficking, and membrane translocation was observed within the septohippocampal system. Given the recent findings that amyloid precursor protein (APP) forms complexes with GABA B receptors (GBRs), it is hypothesized that T-type Ca2+ current reduction, decrease in GABA receptors, and APP destabilization generate complex functional interdependence that can constitute a sophisticated proamyloidogenic environment, which could be of potential relevance in the etiopathogenesis of Alzheimer’s disease (AD). The age-related downregulation of T-type Ca2+ channels in humans goes together with increased Aβ levels that could further inhibit T-type channels and aggravate the proamyloidogenic environment. The mechanistic model presented here sheds new light on recent reports about the potential risks of T-type Ca2+ channel blockers (CCBs) in dementia, as observed upon antiepileptic drug application in the elderly.
LINE-1 Cargo and Reverse Transcriptase Activity Profiles in Extracellular Vesicles from Lung Cancer Cells and Human Plasma
Authors: Bowers Emma C. , Motta Alexandre , Knox Ken , McKay Brian S. , Ramos Kenneth S.
Published: March 2022

Long Interspersed Element-1 (LINE-1) is an oncogenic human retrotransposon that ‘copies and pastes’ DNA into new locations via reverse transcription. Given that enzymatically active LINE-1 can be exported in extracellular vesicles (EVs), and that LINE-1 mRNA and its two encoded proteins, ORF1p and ORF2p, are required for retrotransposition, the present study examined LINE-1 EV loading patterns relative to reverse transcriptase (RT) activity in vivo and in vitro. Density gradient ultracentrifugation identified conserved patterns of LINE-1 mRNA and protein distribution in EVs, with RT activity readily detected in EV fractions containing both LINE-1 mRNA and protein. Unlike whole cell and tissue lysates, the ORF1p in EVs was detected as a dimer. EVs from ostensibly healthy plasma donors showed variable but consistent ORF1p profiles, with residual levels of LINE-1 mRNA measured in some but not all samples. EVs from cancer cell lines had elevated mean LINE-1 levels and 5–85 times greater RT activity than EVs from normal cells or healthy plasma. EV RT activity was associated with EV LINE-1 mRNA content and was highest in cell lines that also expressed an elevated expression of ORF1p and ORF2p. Given that LINE-1 activation is a hallmark of many cancer types, our findings suggest that an EV LINE-1 ‘liquid biopsy’ may be developed to monitor LINE-1 activity during the course of malignant progression.
Laccases and Tyrosinases in Organic Synthesis
Authors: Martínková Ludmila , Křístková Barbora , Křen Vladimír
Published: March 2022

Laccases (Lac) and tyrosinases (TYR) are mild oxidants with a great potential in research and industry. In this work, we review recent advances in their use in organic synthesis. We summarize recent examples of Lac-catalyzed oxidation, homocoupling and heterocoupling, and TYR-catalyzed ortho-hydroxylation of phenols. We highlight the combination of Lac and TYR with other enzymes or chemical catalysts. We also point out the biological and pharmaceutical potential of the products, such as dimers of piceid, lignols, isorhamnetin, rutin, caffeic acid, 4-hydroxychalcones, thiols, hybrid antibiotics, benzimidazoles, benzothiazoles, pyrimidine derivatives, hydroxytyrosols, alkylcatechols, halocatechols, or dihydrocaffeoyl esters, etc. These products include radical scavengers; antibacterial, antiviral, and antitumor compounds; and building blocks for bioactive compounds and drugs. We summarize the available enzyme sources and discuss the scalability of their use in organic synthesis. In conclusion, we assume that the intensive use of laccases and tyrosinases in organic synthesis will yield new bioactive compounds and, in the long-term, reduce the environmental impact of industrial organic chemistry.
Improving Recruitment for a Newborn Screening Pilot Study with Adaptations in Response to the COVID-19 Pandemic
Authors: Wynn Julia , Tavakoli Norma P. , Armstrong Niki , Gomez Jacqueline , Koval Carrie , Lai Christina , Tang Stephanie , Quevedo Prince Andrea , Quevedo Yeyson , Rufino Katrina , Palacio Morales Laura , Pena Angela , Grossman Sharon , Monfiletto Mary , Ruda Erika , Jimenez Vania , Verdade Lorraine , Jones Ashley , Barriga Michelle G. , Karan Nandanee , Puma Alexandria , Sarker Safa , Chin Sarah , Duarte Kelly , Tegay David H. , Bacchus Irzaud , Julooru Rajani , Maloney Breanne , Park Sunju , Saami Akilan M. , Cohen Lilian , Shapiro Natasha , Caggana Michele , Chung Wendy K. , Gruber Dorota
Published: March 2022

Seven months after the launch of a pilot study to screen newborns for Duchenne Muscular Dystrophy (DMD) in New York State, New York City became an epicenter of the coronavirus disease 2019 (COVID-19) pandemic. All in-person research activities were suspended at the study enrollment institutions of Northwell Health and NewYork-Presbyterian Hospitals, and study recruitment was transitioned to 100% remote. Pre-pandemic, all recruitment was in-person with research staff visiting the postpartum patients 1–2 days after delivery to obtain consent. With the onset of pandemic, the multilingual research staff shifted to calling new mothers while they were in the hospital or shortly after discharge, and consent was collected via emailed e-consent links. With return of study staff to the hospitals, a hybrid approach was implemented with in-person recruitment for babies delivered during the weekdays and remote recruitment for babies delivered on weekends and holidays, a cohort not recruited pre-pandemic. There was a drop in the proportion of eligible babies enrolled with the transition to fully remote recruitment from 64% to 38%. In addition, the proportion of babies enrolled after being approached dropped from 91% to 55%. With hybrid recruitment, the proportion of eligible babies enrolled (70%) and approached babies enrolled (84%) returned to pre-pandemic levels. Our experience adapting our study during the COVID-19 pandemic led us to develop new recruitment strategies that we continue to utilize. The lessons learned from this pilot study can serve to help other research studies adapt novel and effective recruitment methods.
Mechanical Properties of Cold Mix Asphalt (CMA) Mixed with Recycled Asphalt Pavement
Authors: Chegenizadeh Amin , Tufilli Anthony , Arumdani Indah Sekar , Budihardjo Mochamad Arief , Dadras Ethan , Nikraz Hamid
Published: March 2022

From the perspective of sustainability and environmental concerns, the use of cold-mix asphalt (CMA) and recycled asphalt pavement (RAP) is more advantageous than the use of hot mix asphalt (HMA) and warm mix asphalt (WMA). Some researchers used a mixture of CMA and RAP to improve the mechanical properties of pavement and made it more economical. However, only a few studies have focused on using a high content of RAP—particularly 100% RAP—as the virgin aggregate. Therefore, this study aims to analyse cold-mix asphalt (CMA) using 100% recycled asphalt pavement (RAP) instead of virgin aggregate raw materials and to determine the best mixture for the production of environmentally friendly asphalt. It is necessary to investigate the performance of CMA mixed with RAP in terms of the resilient modulus, indirect tensile strength, fatigue and rutting resistance. In this study, the percentage of bitumen emulsions added is 2%, 2.5%, 3%, 3.5%, and 4%, along with 100% RAP material. The results indicate that the fatigue life of the RAP mixture increased by 49.34% with the addition of bitumen emulsion (BE) from 2% to 4%, while the wheel tracking test experienced a decrease in rutting depth along with an increase in BE dose of 4%, which was 9 mm. The mixture containing 4% asphalt emulsion has the best performance. The results suggested that increasing the BE dosage increases the resistance against rutting and fatigue.
The Effect of Natural-Based Formulation (NBF) on the Response of RAW264.7 Macrophages to LPS as an In Vitro Model of Inflammation
Authors: Monga Sheelu , Fares Basem , Yashaev Rami , Melamed Dov , Kahana Meygal , Fares Fuad , Weizman Abraham , Gavish Moshe
Published: March 2022

Macrophages are some of the most important immune cells in the organism and are responsible for creating an inflammatory immune response in order to inhibit the passage of microscopic foreign bodies into the blood stream. Sometimes, their activation can be responsible for chronic inflammatory diseases such as asthma, tuberculosis, hepatitis, sinusitis, inflammatory bowel disease, and viral infections. Prolonged inflammation can damage the organs or may lead to death in serious conditions. In the present study, RAW264.7 macrophages were exposed to lipopolysaccharide (LPS; 20 ng/mL) and simultaneously treated with 20 µg/mL of natural-based formulation (NBF), mushroom–cannabidiol extract). Pro-inflammatory cytokines, chemokines, and other inflammatory markers were analyzed. The elevations in the presence of interleukin-6 (IL-6), cycloxygenase-2 (COX-2), C-C motif ligand-5 (CCL5), and nitrite response, following exposure to LPS, were completely inhibited by NBF administration. IL-1β and tumor necrosis factor alpha (TNF-α) release were inhibited by 3.9-fold and 1.5-fold, respectively. No toxic effect of NBF, as assessed by lactate dehydrogenase (LDH) release, was observed. Treatment of the cells with NBF significantly increased the mRNA levels of TLR2, and TLR4, but not NF-κB. Thus, it appears that the NBF possesses anti-inflammatory and immunomodulatory effects which can attenuate the release of pro-inflammatory markers. NBF may be a candidate for the treatment of acute and chronic inflammatory diseases and deserves further investigation.
A Simple Algorithm Using Ventilator Parameters to Predict Successfully Rapid Weaning Program in Cardiac Intensive Care Unit Patients
Authors: Chen Wei-Teing , Huang Hai-Lun , Ko Pi-Shao , Su Wen , Kao Chung-Cheng , Su Sui-Lung
Published: March 2022

Background: Ventilator weaning is one of the most significant challenges in the intensive care unit (ICU). Approximately 30% of patients fail to wean, resulting in prolonged use of ventilators and increased mortality. There are numerous high-performance prediction models available today, but they require a large number of parameters to predict and are thus impractical in clinical practice. Objectives: This study aims to create an artificial intelligence (AI) model for predicting weaning time and to identify the most simplified key predictors that will allow the model to achieve adequate accuracy with as few parameters as possible. Methods: This is a retrospective study of to-be-weaned patients (n = 1439) hospitalized in the cardiac ICU of Cheng Hsin General Hospital’s Department of Cardiac Surgery from November 2018 to August 2020. The patients were divided into two groups based on whether they could be weaned within 24 h (i.e., “patients weaned within 24 h” (n = 1042) and “patients not weaned within 24 h” (n = 397)). Twenty-eight variables were collected including demographic characteristics, arterial blood gas readings, and ventilation set parameters. We created a prediction model using logistic regression and compared it to other machine learning techniques such as decision tree, random forest, support vector machine (SVM), extreme gradient boosting, and artificial neural network. Forward, backward, and stepwise selection methods were used to identify significant variables, and the receiver operating characteristic curve was used to assess the accuracy of each AI model. Results: The SVM [receiver operating characteristic curve (ROC-AUC) = 88%], logistic regression (ROC-AUC = 86%), and XGBoost (ROC-AUC = 85%) models outperformed the other five machine learning models in predicting weaning time. The accuracies in predicting patient weaning within 24 h using seven variables (i.e., expiratory minute ventilation, expiratory tidal volume, ventilation rate set, heart rate, peak pressure, pH, and age) were close to those using 28 variables. Conclusions: The model developed in this research successfully predicted the weaning success of ICU patients using a few and easily accessible parameters such as age. Therefore, it can be used in clinical practice to identify difficult-to-wean patients to improve their treatment.
Changes in Facial Profile after Modified Anterior Maxillary Subapical Osteotomy
Authors: Chen Chun-Ming , Chou Szu-Ting , Chen Shih-Chieh , Pan Chin-Yun , Hsu Kun-Jung , Tseng Yu-Chuan
Published: March 2022

(1) Background: This study explored the effects of modified anterior maxillary subapical osteotomy (AMSO) on facial profile changes in patients with bimaxillary protrusion. (2) Methods: Cephalograms of patients were collected preoperatively and over 2 months postoperatively. The following landmarks were recorded: pronasale (Prn), subnasale (Sn), labrale superius (Ls), anterior nasal spine (ANS), and incisor superius (Is). The following distances and angles were measured: ANS–Prn, ANS–Sn, ANS–Ls, Is–Sn, Is–Ls, SNA angle, and nasolabial (NLA) angle. (3) Results: Is and ANS were significantly retracted by 7.3 and 2.3 mm, respectively. Soft tissue landmarks (Prn, Sn, and Ls) were significantly retracted (1.2, 1.6, 4.4 mm, respectively). Postoperative changes in soft/hard tissue ratios were 0.54, 0.72, 0.31, and 0.60 for Prn/ANS, Sn/ANS, ANS/Is, and Ls/Is, respectively. The NLA angle was increased significantly by 7.1°. (4) Conclusions: The horizontal soft/hard tissue ratios of Sn/Is, ANS/Is, and Ls/Is were 0.22, 0.31, and 0.60, respectively. The NLA angle was increased significantly by 7.1°. The modified AMSO provides an increased blood supply, allows for direct vision, and results in fewer complications than other AMSO methods.
Approaching Composition as Showing–Telling through Translanguaging: Weaving Multilingualism, Multimodality, and Multiliteracies in a Digital Collage Proyecto Final
Authors: Prada Josh
Published: March 2022

Couched in theories of translanguaging, multimodality, and multiliteracies, this article explores digital compositions (i.e., digital collages) as spaces for identity representation through the proyectos finales produced by 22 students in a Spanish composition class for heritage/native speakers in a U.S. university. Each digital collage was accompanied by two written documents: one describing the processes leading to its creation, and another one explaining the meaning of the collage and its components. Qualitative content analysis was used to investigate the submissions, with particular attention paid to instances of identity, experience, and self-representation through complex orchestrations of flexible multilingual and multimodal meaning- and sense-making. The proyecto final is discussed in terms of the curricular innovation for courses designed for racialized language-minoritized multilingual students, describing the nature and affordances of translanguaging in this context, and advancing an approach to digital composing as showing–telling.
Electric and Dielectric Properties in Low-Frequency Fields of Composites Consisting of Silicone Rubber and Al Particles for Flexible Electronic Devices
Authors: Teusdea Alexandrina , Malaescu Iosif , Sfirloaga Paula , Marin Catalin Nicolae
Published: March 2022

Understanding the electrical conduction and dielectric polarization properties of elastomer-based composites is important for the design of flexible and elastic electronic devices and circuits. Five samples were manufactured by mixing silicone rubber (RTV-530) with Al particles in different volume fractions, x equal to 0%, 0.5%, 1%, 2.5% and 5.1%. Using the complex impedance measurements, the electric modulus, M, the electrical conductivity, σ, and the dielectric permittivity, ε, over the frequency range 100 Hz–200 kHz were analyzed. The electrical conductivity spectrum, σ(f), follows the Jonscher universal law and the DC conductivity of the samples, σDC, increases from 2.637·10−8 S/m to 5.725·10−8 S/m, with increasing x from, 0 to 5.1%. The conduction process was analyzed in terms of Mott’s variable-range-hopping (VRH) model. The hopping distance of the charge carriers, Rh decreases with increasing x, from 7.30 nm (for x = 0) to 5.92 nm (for x = 5.1%). The frequency dependence of permittivity, ε(f) = ε′(f) − iε″(f), reveals a relaxation process with the maximum of ε″(f) shifting from 301 Hz to 385 Hz and values of ε′(f) increasing with the increase of x.
Design, Synthesis and Adsorption Evaluation of Bio-Based Lignin/Chitosan Beads for Congo Red Removal
Authors: Han Xiaobing , Li Rong , Miao Pengpai , Gao Jie , Hu Guowen , Zhao Yuan , Chen Tao
Published: March 2022

The morphology and intermolecular interaction are two of the most important factors in the design of highly efficient dye adsorbent in the industry. Millimeter-sized, bead-type, bio-based lignin/chitosan (Lig/CS) adsorbent was designed for the removal of Congo red (CR), based on the electrostatic attraction, π-π stacking, and hydrogen bonding, which were synthesized through the emulsification of the chitosan/lignin mixture followed by chemical cross-linking. The effects of the lignin/chitosan mass ratio, initial pH, temperature, concentration, and contact time on the adsorption were thoroughly investigated. The highest adsorption capacity (173 mg/g) was obtained for the 20 wt% Lig/CS beads, with a removal rate of 86.5%. To investigate the adsorption mechanism and recyclability, an evaluation of the kinetic model and an adsorption/desorption experiment were conducted. The adsorption of CR on Lig/CS beads followed the type 1 pseudo-second-order model, and the removal rate for CR was still above 90% at five cycles.
Low-Velocity Impact Response on Glass Fiber Reinforced 3D Integrated Woven Spacer Sandwich Composites
Authors: Rahman Mahfuz Bin , Zhu Lvtao
Published: March 2022

This study presents an experimental investigation on the low-velocity impact response of three-dimensional integrated woven spacer sandwich composites made of high-performance glass fiber reinforced fabric and epoxy resin. 3D integrated woven spacer sandwich composites with five different specifications were produced using a hand lay-up process and tested under low-velocity impact with energies of 5 J, 10 J, and 15 J. The results revealed that the core pile’s heights and diverse impact energies significantly affect the stiffness and energy absorption capacity. There is no significant influence of face sheet thickness on impact response. Moreover, the damage morphologies of 3D integrated woven spacer sandwich composites under different impact energies were analyzed by simple visualization of the specimen. Different damage and failure mechanisms were observed, including barely visible damage, visible damage, and clearly visible damage. Moreover, it was noticed that the damage of 3D integrated woven spacer sandwich composites samples only constraints to the impacted area and does not affect the integrity of the samples.
Hydrophobic Recovery of PDMS Surfaces in Contact with Hydrophilic Entities: Relevance to Biomedical Devices
Authors: Tsuzuki Tomoo , Baassiri Karine , Mahmoudi Zahra , Perumal Ayyappasamy Sudalaiyadum , Rajendran Kavya , Rubies Gala Montiel , Nicolau Dan V.
Published: March 2022

Polydimethylsiloxane (PDMS), a silicone elastomer, is increasingly being used in health and biomedical fields due to its excellent optical and mechanical properties. Its biocompatibility and resistance to biodegradation led to various applications (e.g., lung on a chip replicating blood flow, medical interventions, and diagnostics). The many advantages of PDMS are, however, partially offset by its inherent hydrophobicity, which makes it unsuitable for applications needing wetting, thus requiring the hydrophilization of its surface by exposure to UV or O2 plasma. Yet, the elastomeric state of PDMS translates in a slow, hours to days, process of reducing its surface hydrophilicity—a process denominated as hydrophobic recovery. Using Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM), the present study details the dynamics of hydrophobic recovery of PDMS, on flat bare surfaces and on surfaces embedded with hydrophilic beads. It was found that a thin, stiff, hydrophilic, silica film formed on top of the PDMS material, following its hydrophilization by UV radiation. The hydrophobic recovery of bare PDMS material is the result of an overlap of various nano-mechanical, and diffusional processes, each with its own dynamics rate, which were analyzed in parallel. The hydrophobic recovery presents a hysteresis, with surface hydrophobicity recovering only partially due to a thin, but resilient top silica layer. The monitoring of hydrophobic recovery of PDMS embedded with hydrophilic beads revealed that this is delayed, and then totally stalled in the few-micrometer vicinity of the embedded hydrophilic beads. This region where the hydrophobic recovery stalls can be used as a good approximation of the depth of the resilient, moderately hydrophilic top layer on the PDMS material. The complex processes of hydrophilization and subsequent hydrophobic recovery impact the design, fabrication, and operation of PDMS materials and devices used for diagnostics and medical procedures. Consequently, especially considering the emergence of new surgical procedures using elastomers, the impact of hydrophobic recovery on the surface of PDMS warrants more comprehensive studies.
Fragment-Resistant Property Optimization within Ballistic Inserts Obtained on the Basis of Para-Aramid Materials
Authors: Kośla Katarzyna , Kubiak Paweł , Łandwijt Marcin , Urbaniak Wioleta , Kucharska-Jastrząbek Agnieszka
Published: March 2022

A high protection level without an excessive weight is a basic assumption in the design of modern armors and protection systems. Optimizing armors is a task of development of the utmost importance, and is the subject of the work contained within this article. Optimization of ballistic inserts was carried out using multicriterial analysis (MCA), which enables the selection of the optimal composition, taking into account properties such as ballistic resistance, physicomechanical, and/or functional properties. For this purpose, various types of composite systems were produced and tested in terms of their fragment-resistant properties according to STANAG 2920 and the composite areal density of different ballistic inserts: Soft inserts made of Twaron® para-aramid sheets, hard ballistic inserts made of multilayer hot-pressed preimpregnated sheets, and hybrid hard ballistic inserts prepared on the basis of multilayer hot-pressed preimpregnated sheets and ceramics. The application of MCA and performance of experimental fragment resistance tests for a wide spectrum of para-aramid inserts are part of the novelty of this work. The obtained test results showed that depending on the composition of the composite system, we could obtain a wide range of fragmentation resistance in the range of 300 to >1800 m/s, which depended on the areal density and type of composite system used. The results also confirmed that MCA is a good computational tool to select the optimal design of para-aramid ballistic inserts.
Correction: Chen et al. The Effect of High-Quality RDX on the Safety and Mechanical Properties of Pressed PBX. Materials 2022, 15, 1185
Authors: Chen Shixiong , Qian Hua , Liu Bingxin , Xu Feiyang , Rui Jiuhou , Liu Dabin
Published: March 2022

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Production and Characterization of the Third-Generation Oxide Nanotubes on Ti-13Zr-13Nb Alloy
Authors: Łosiewicz Bożena , Skwarek Sandra , Stróż Agnieszka , Osak Patrycja , Dudek Karolina , Kubisztal Julian , Maszybrocka Joanna
Published: March 2022

In the group of vanadium-free titanium alloys used for applications for long-term implants, the Ti-13Zr-13Nb alloy has recently been proposed. The production of a porous layer of oxide nanotubes (ONTs) with a wide range of geometries and lengths on the Ti-13Zr-13Nb alloy surface can increase its osteoinductive properties and enable intelligent drug delivery. This work concerns developing a method of electrochemical modification of the Ti-13Zr-13Nb alloy surface to obtain third-generation ONTs. The effect of the anodizing voltage on the microstructure and thickness of the obtained oxide layers was conducted in 1 M C2H6O2 + 4 wt% NH4F electrolyte in the voltage range 5–35 V for 120 min at room temperature. The obtained third-generation ONTs were characterized using SEM, EDS, SKP, and 2D roughness profiles methods. The preliminary assessment of corrosion resistance carried out in accelerated corrosion tests in the artificial atmosphere showed the high quality of the newly developed ONTs and the slight influence of neutral salt spray on their micromechanical properties.
Suitability of Constitutive Models of the Structural Concrete Codes When Applied to Polyolefin Fibre Reinforced Concrete
Authors: Enfedaque Alejandro , Suárez Fernando , Alberti Marcos G. , Gálvez Jaime C.
Published: March 2022

The use of fibres as structural reinforcement in concrete is included in standards, providing guidelines to reproduce their behaviour, which have been proven adequate when steel fibres are used. Nevertheless, in recent years new materials, such as polyolefin fibres, have undergone significant development as concrete reinforcement. This work gives insight on how suitable the constitutive models proposed by the Model Code 2010 (MC2010) are in the case of such polymer fibres. A set of numerical models has been carried out to reproduce the material behaviour proposed by the MC2010 and the approach based on the softening function proposed by the authors. The results show remarkable differences between the experimental results and the numerical simulations when the constitutive models described in the MC2010 are employed for different polyolefin fibre reinforced concrete mixes, while the material behaviour can be reproduced with greater accuracy if the softening function proposed by the authors is employed when this type of macro-polymer fibres is used. Moreover, the relatively complex behaviour of polyolefin fibre reinforced concrete may be reproduced by using such constitutive model.
A Numerical Analysis of Ductile Deformation during Nanocutting of Silicon Carbide via Molecular Dynamics Simulation
Authors: Liu Bing , Li Xiaolin , Kong Ruijie , Yang Haijie , Jiang Lili
Published: March 2022

As a typical third-generation semiconductor material, silicon carbide (SiC) has been increasingly used in recent years. However, the outstanding performance of SiC component can only be obtained when it has a high-quality surface and low-damage subsurface. Due to the hard–brittle property of SiC, it remains a challenge to investigate the ductile machining mechanism, especially at the nano scale. In this study, a three-dimensional molecular dynamics (MD) simulation model of nanometric cutting on monocrystalline 3C-SiC was established based on the ABOP Tersoff potential. Multi-group MD simulations were performed to study the removal mechanism of SiC at the nano scale. The effects of both cutting speed and undeformed cutting thickness on the material removal mechanism were considered. The ductile machining mechanism, cutting force, hydrostatic pressure, and tool wear was analyzed in depth. It was determined that the chip formation was dominated by the extrusion action rather than the shear theory during the nanocutting process. The performance and service life of the diamond tool can be effectively improved by properly increasing the cutting speed and reducing the undeformed cutting thickness. Additionally, the nanometric cutting at a higher cutting speed was able to improve the material removal rate but reduced the quality of machined surface and enlarged the subsurface damage of SiC. It is believed that the results can promote the level of ultraprecision machining technology.
Effectiveness of Some Novel Ionic Liquids on Mild Steel Corrosion Protection in Acidic Environment: Experimental and Theoretical Inspections
Authors: Al-Rashed Osama , Abdel Nazeer Ahmed
Published: March 2022

Three ionic liquids (ILs)—1-butyl-1-methyl-pyrrolidinium Imidazolate (BMPyrIM), 1-butyl-3-methyl-imidazolium Imidazolate (BMImIM), and bis(1-butyl-3-methyl-imidazolium Imidazolate) (BBMImIM)—were synthesized and examined experimentally and theoretically as potential inhibitors for mild steel corrosion in HCl (1.0 M) solution. To our knowledge, two of the ILs successfully synthesized in our laboratory named BMPyrIM and BBMImIM are novel. Different electrochemical (potentiodynamic polarization (PDP), electrochemical impedance spectroscopy (EIS)), surface and structural (scanning electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDS), Atomic force microscopy (AFM) and Fourier Transform Infrared Spectroscopy (FTIR)) and theoretical (Density functional theory (DFT)) techniques were utilized to confirm their use as efficient environmentally safe inhibitors. These ionic liquids were designed to study the cation effect (imidazolium and pyrrolidinium) and the dimeric effect of the imidazolium-based IL. A pronounced inhibiting effect was recorded using the optimum concentration (5 × 10−3 M) of BBMImIM with protection efficiency of 98.6% compared to 94.3% and 92.4% for BMImIM and BMPyrIM, respectively. The investigated ILs act as a mixed-type corrosion inhibitors and their protection obeys Langmuir adsorption isotherm. The results obtained by SEM, EDS and AFM confirmed the mild steel protection by the formation of protective film of the ILs on the steel surface resulted in less damaged surfaces compared with the blank solution. Furthermore, quantum chemical calculations illustrated the electronic structure of the investigated ILs and their optimized adsorptiοn configurations on mild steel surface. The findings from the different techniques helped to provide a supported interpretation of the inhibition mechanism.
Functioning of Heat Accumulating Composites of Carbon Recyclate and Phase Change Material
Authors: Musiał Michał , Pękala Agnieszka
Published: March 2022

The article presents the results of experimental research together with the development of a response function presenting the thermal functioning of a new composite of a phase change material with carbon recyclate. The empirical research proved the improvement of the thermal functioning of the phase change material as a result of modifying its structure with carbon-based recycling material. The conducted experimental tests and statistical analysis proved that the obtained innovative composite is characterized by a more effective distribution of stored heat than the pure phase change material, which resulted in reduction of the heating and cooling time of the package by 10 min. The obtained innovative composite can improve the thermal efficiency of short-term heat storage systems, both in building components and in elements of heating and cooling systems, and translates into their increase in thermal efficiency.
Immobilization of Hexavalent Chromium Using Self-Compacting Soil Technology
Authors: Rudzionis Zymantas , Navickas Arunas Aleksandras , Stelmokaitis Gediminas , Ivanauskas Remigijus
Published: March 2022

A study of immobilization of hexavalent chromium in the form of Na2CrO4 salt by self-compacting soils (SCS) is presented. Carbofill E additive was used as SCS binder. The efficiency of immobilization of Cr (VI) was evaluated by washing out chromium compounds from SCS samples. The influence of the nature of the soil and the content of Carbofill E and Na2CrO4 in the SCS samples on the efficiency of Cr (VI) immobilization was studied. It was found that the nature of the soil and the content of Carbofill E in the SCS samples affect the immobilization of Cr (VI). Moreover, increasing the Carbofill E content in SCS samples further increases Cr (VI) immobilization. X-ray diffraction studies of the samples with immobilized hexavalent chromium showed that part of the sample transforms from a readily soluble form of salt into oxide forms of chromium and calcium-chromium, which are practically insoluble in water.
Fabrication of MnCuNiFe–CuAlNiFeMn Gradient Alloy by Laser Engineering Net Shaping System
Authors: Yan Kuo , Lin Zaiwen , Chen Meng , Wang Yuren , Wang Jun , Jiang Heng
Published: March 2022

Marine noise pollution generated by propellers is of wide concern. Traditional propeller materials (nickel–aluminum bronze (NAB) alloys) can no longer meet the requirements for reducing shaft vibration. However, the Mn–Cu alloy developed to solve the problem of propeller vibration is affected by seawater corrosion, which greatly limits the application of the alloy in the field of marine materials. In this study, the M2052–NAB gradient alloy was developed for the first time using LENS technology to improve the corrosion resistance while retaining the damping properties of the M2052 alloy. We hope this alloy can provide a material research basis for the development of low-noise propellers. This study shows that, after solution-aging of M2052 alloy as the matrix, the martensitic transformation temperature increased to approach the antiferromagnetic transformation temperature, which promoted twinning and martensitic transformation. The aging process also eliminated dendrite segregation, promoted the equiaxed γ-MnCu phase, and increased the crystal size to reduce the number of dislocations, resulting in obvious modulus softening of the alloy. NAB after deposition had higher hardness and good corrosion resistance than the as-cast alloy, which offers good corrosion protection for the M2052 alloy. This research provides new material options for the field of shipbuilding.
A Comprehensive Survey on Fault Tolerance in Multiphase AC Drives, Part 2: Phase and Switch Open-Circuit Faults
Authors: Yepes Alejandro G. , Gonzalez-Prieto Ignacio , Lopez Oscar , Duran Mario J. , Doval-Gandoy Jesus
Published: March 2022

Multiphase machines are very convenient for applications that require high reliability. In this two-part survey, the state of the art about fault tolerance in multiphase drives is reviewed. In Part 1, an overview including numerous fault types was presented, along with fundamental notions about multiphase drives. Here, in Part 2, the focus is placed on phase/switch open-circuit (OC) faults in particular, which have received the most attention in the literature. Phase OC failures involve OCs in stator phases or in converter-machine connections, and switch/diode OCs are frequently dealt with similarly or identically. Thanks to the phase redundancy of multiphase drives, their operation can be satisfactorily continued under a certain number of OCs. Nonetheless, the procedure to follow for this purpose is far from unique. For given OC fault conditions, numerous fault-tolerant possibilities can be found in the literature, each of them with different advantages and disadvantages. Moreover, a great variety of methods have also been devised to detect and diagnose phase/switch OC failures so that, as soon as possible, the most appropriate fault-tolerance measures are applied. Thus, given the broad literature about tolerance to phase/switch OC faults in multiphase drives, the survey presented here is expected to be of great interest for the research community and industry.