bluuebunny/crossref_metadata_2025 huggingface dataset:
Growth and Photosynthetic Responses of Cowpea Genotypes under Waterlogging at the Reproductive Stage
Authors: Olorunwa Omolayo J. , Adhikari Bikash , Brazel Skyler , Shi Ainong , Popescu Sorina C. , Popescu George V. , Barickman T. Casey
Published: September 2022

Waterlogging is an important environmental stress limiting the productivity of crops worldwide. Cowpea (Vigna unguiculata L.) is particularly sensitive to waterlogging stress during the reproductive stage, with a consequent decline in pod formation and yield. However, little is known about the critical processes underlying cowpea’s responses to waterlogging during the reproductive stage. Thus, we investigated the key parameters influencing carbon fixation, including stomatal conductance (gs), intercellular CO2 concentration, chlorophyll content, and chlorophyll fluorescence, of two cowpea genotypes with contrasting waterlogging tolerance. These closely related genotypes have starkly contrasting responses to waterlogging during and after 7 days of waterlogging stress (DOW). In the intolerant genotype (‘EpicSelect.4’), waterlogging resulted in a gradual loss of pigment and decreased photosynthetic capacity as a consequent decline in shoot biomass. On the other hand, the waterlogging-tolerant genotype (‘UCR 369’) maintained CO2 assimilation rate (A), stomatal conductance (gs), biomass, and chlorophyll content until 5 DOW. Moreover, there was a highly specific downregulation of the mesophyll conductance (gm), maximum rate of Rubisco (Vcmax), and photosynthetic electron transport rate (Jmax) as non-stomatal limiting factors decreasing A in EpicSelect.4. Exposure of EpicSelect.4 to 2 DOW resulted in the loss of PSII photochemistry by downregulating the PSII quantum yield (Fv/Fm), photochemical efficiency (ΦPSII), and photochemical quenching (qP). In contrast, we found no substantial change in the photosynthesis and chlorophyll fluorescence of UCR 369 in the first 5 DOW. Instead, UCR 369 maintained biomass accumulation, chlorophyll content, and Rubisco activity, enabling the genotype to maintain nutrient absorption and photosynthesis during the early period of waterlogging. However, compared to the control, both cowpea genotypes could not fully recover their photosynthetic capacity after 7 DOW, with a more significant decline in EpicSelect.4. Overall, our findings suggest that the tolerant UCR 369 genotype maintains higher photosynthesis under waterlogging stress attributable to higher photochemical efficiency, Rubisco activity, and less stomatal restriction. After recovery, the incomplete recovery of A can be attributed to the reduced gs caused by severe waterlogging damage in both genotypes. Thus, promoting the rapid recovery of stomata from waterlogging stress may be crucial for the complete restoration of carbon fixation in cowpeas during the reproductive stage.
The Role of Alternative Electron Pathways for Effectiveness of Photosynthetic Performance of Arabidopsis thaliana, Wt and Lut2, under Low Temperature and High Light Intensity
Authors: Popova Antoaneta V. , Stefanov Martin , Ivanov Alexander G. , Velitchkova Maya
Published: September 2022

A recent investigation has suggested that the enhanced capacity for PSI-dependent cyclic electron flow (CEF) and PSI-dependent energy quenching that is related to chloroplast structural changes may explain the lower susceptibility of lut2 to combined stresses—a low temperature and a high light intensity. The possible involvement of alternative electron transport pathways, proton gradient regulator 5 (PGR5)-dependent CEF and plastid terminal oxidase (PTOX)-mediated electron transfer to oxygen in the response of Arabidopsis plants—wild type (wt) and lut2—to treatment with these two stressors was assessed by using specific electron transport inhibitors. Re-reduction kinetics of P700+ indicated that the capacity for CEF was higher in lut2 when this was compared to wt. Exposure of wt plants to the stress conditions caused increased CEF and was accompanied by a substantial raise in PGR5 and PTOX quantities. In contrast, both PGR5 and PTOX levels decreased under the same stress conditions in lut2, and inhibiting PGR5-dependent pathway by AntA did not exhibit any significant effects on CEF during the stress treatment and recovery period. Electron microscopy observations demonstrated that under control conditions the degree of grana stacking was much lower in lut2, and it almost disappeared under the combined stresses, compared to wt. The role of differential responses of alternative electron transport pathways in the acclimation to the stress conditions that are studied is discussed.
The Biomechanical Screening Game between Visitor Power and Staminode Operative Strength of Delphinium caeruleum (Ranunculaceae)
Authors: Hou Qin-Zheng , Shao Wen-Juan , Ehmet Nurbiye , Yang Guang , Zhong Yu-Qin , Min Wen-Rui , Xu Yi-Fan , Gao Ruo-Chun
Published: September 2022

During the evolution of angiosperm flowers, some floral traits may undergo certain changes in order to participate in screening. The stamens and pistils of Delphinium caeruleum are covered by two “door-like” staminodes, the evolutionary function of which, however, is quite unknown. In this study, we investigated whether D. caeruleum staminodes acted as visitor filters by assessing the respective strengths of staminodes and visitor insects (six bee species). We measured the operative strength required to open the staminodes and the strength that insects were capable of exerting using a biological tension sensor. Furthermore, we compared the strength required to open staminodes at different phases of the flowering period (male and female phases) and the strength of different visitors (visitors and non-visitors of D. caeruleum). The results showed that the strength needed to open staminodes in the male phase was significantly higher than that in the female phase. There was no significant difference between the strength exerted by visitors and required by staminodes of D. caeruleum in the male phase, but the visitor strength was significantly higher than that required to open staminodes in the female phase flowers. The strength of non-visitors was significantly lower than that required to open staminodes in the male phase. Furthermore, there was a significant positive association between the strength and the body weight of the bees. These results highlighted the observation that only strong visitors could press the two staminodes to access the sex organs and achieve successful pollination. Furthermore, these results revealed the function of pollinator screening by the staminodes of D. caeruleum. The biomechanical approach to the study of flowers allowed us to address relevant ecological and evolutionary questions of the plant–pollinator interaction and explore the functional modules within the flower structure in other plant species.
Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans
Authors: Patyra Andrzej , Kołtun-Jasion Małgorzata , Jakubiak Oktawia , Kiss Anna Karolina
Published: September 2022

Lignans are a group of natural polyphenols present in medicinal plants and in plants which are a part of the human diet for which more and more pharmacological activities, such as antimicrobial, anti-inflammatory, hypoglycemic, and cytoprotective, are being reported. However, it is their cytotoxic activities that are best understood and which have shed light on this group. Two anticancer drugs, etoposide, and teniposide, were derived from a potent cytotoxic agent—podophyllotoxin from the roots of Podophyllum peltatum. The evidence from clinical and observational studies suggests that human microbiota metabolites (enterolactone, enterodiol) of dietary lignans (secoisolariciresinol, pinoresinol, lariciresinol, matairesinol, syringaresinol, medioresinol, and sesamin) are associated with a reduced risk of some hormone-dependent cancers. The biological in vitro, pharmacological in vivo investigations, and clinical studies demand significant amounts of pure compounds, as well as the use of well-defined and standardized extracts. That is why proper extract preparation, optimization of lignan extraction, and identification are crucial steps in the development of lignan use in medicine. This review focuses on lignan extraction, purification, fractionation, separation, and isolation methods, as well as on chromatographic, spectrometric, and spectroscopic techniques for their qualitative and quantitative analysis.
Population-Specific Plant-To-Plant Signaling in Wild Lima Bean
Authors: Grof-Tisza Patrick , Morelon Stéphanie , Desurmont Gaylord A. , Benrey Betty
Published: September 2022

The exposure to volatiles from damaged plants can increase the resistance of the neighboring plants to herbivores. Studies have demonstrated that the strength of this response depends on the level of relatedness between the interacting plants. Indeed, a field study with Phaseolus lunatus found that the responses to induced volatiles were population-specific; individuals exposed to damaged conspecifics from the ‘local’ population exhibited greater resistance to herbivores than those exposed to damaged conspecifics from ‘foreign’ populations. Here, we repeated this study in the laboratory by placing undamaged plants near damaged plants from either their local or a foreign population. The former plants experienced less herbivory than the latter after a subsequent challenge by a generalist herbivore. To understand the role of the volatiles underlying this observed specificity, we explored the variability in the constitutively released volatiles and volatiles released after mechanical or herbivore damage among the three tested populations of P. lunatus. The total volatile emissions were 5× and 10× higher from the mechanically and herbivore-damaged plants, respectively, compared to the undamaged plants. The populations differed in their relative ratios of dominant constitutive compounds, but no pattern was observed that could explain the differential responses to induced volatiles among the populations. Overall, this study confirms the population-specific volatile-mediated interactions in P. lunatus.
Opuntia spp. in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 2
Authors: Madrigal-Santillán Eduardo , Portillo-Reyes Jacqueline , Madrigal-Bujaidar Eduardo , Sánchez-Gutiérrez Manuel , Izquierdo-Vega Jeannett A. , Izquierdo-Vega Julieta , Delgado-Olivares Luis , Vargas-Mendoza Nancy , Álvarez-González Isela , Morales-González Ángel , Morales-González José A.
Published: September 2022

Plants of the genus Opuntia spp are widely distributed in Africa, Asia, Australia and America. Specifically, Mexico has the largest number of wild species; mainly O. streptacantha, O. hyptiacantha, O. albicarpa, O. megacantha and O. ficus-indica. The latter being the most cultivated and domesticated species. Its main bioactive compounds include pigments (carotenoids, betalains and betacyanins), vitamins, flavonoids (isorhamnetin, kaempferol, quercetin) and phenolic compounds. Together, they favor the different plant parts and are considered phytochemically important and associated with control, progression and prevention of some chronic and infectious diseases. Part 1 collected information on its preventive actions against atherosclerotic cardiovascular diseases, diabetes and obesity, hepatoprotection, effects on human infertility and chemopreventive capacity. Now, this second review (Part 2), compiles the data from published research (in vitro, in vivo, and clinical studies) on its neuroprotective, anti-inflammatory, antiulcerative, antimicrobial, antiviral potential and in the treatment of skin wounds. The aim of both reviews is to provide scientific evidences of its beneficial properties and to encourage health professionals and researchers to expand studies on the pharmacological and therapeutic effects of Opuntia spp.
Bio-Based Rigid Polyurethane Foams Modified with C-MOF/MWCNTs and TBPBP as Building Insulation Materials: Synergistic Effect and Corresponding Mechanism for Enhancing Fire and Smoke Safety
Authors: Bo Guangxu , Xu Xiaoling , Tian Xiaoke , Yan Jinyong , Su Xingjian , Yan Yunjun
Published: September 2022

Rigid polyurethane foams (RPUFs) as building insulation materials quickly burn and release a lot of heat, smoke, and carbon monoxide, and cause human safety risk and severe environmental pollution. To mitigate these disadvantages, MOF/MWCNTs were fabricated via mixing Cu ions’ partly substituted framework of ZIF-67 and MWCNTs, and further calcinated MOF/MWCNTs (C-MOF/MWCTs) was newly generated by calcinating MOF/MWCNTs in air. Then, MOF/MWCNTs and C-MOF/MWCNTs were respectively employed together with a phosphorus–nitrogen-containing reactive flame retardant (TBPBP) to prepare renewable bio-based rigid polyurethane foam, including RPUF-T/MOF/MWCNTs 2 and RPUF-T/C-MOF/MWCNTs 2. The characterization results showed that RPUF-T/C-MOF/MWCNTs 2 had better performance than RPUF-T/MOF/MWCNTs 2 and neat RPUF. Compared to neat RPUF, the compressive strength, limiting oxygen index value, and the mass char residue in cone calorimetry test of RPUF-T/C-MOF/MWCNTs 2, respectively, were increased by 105.93%, 46.35%, and 347.32%; meanwhile, the total heat release rate, total smoke production, total carbon monoxide product, and total carbon dioxide product were reduced by 47.97%, 50.46%, 41.38%, 43.37%, respectively. This study provides a referable method for preparing RPUFs with good physical properties, fire, and smoke safety, which is favorable for human safety and environmental protection as new building insulation materials.
Study of the Potential Accumulation of the Pesticide Alpha-Endosulfan by Microplastics in Water Systems
Authors: Martinho Sílvia D. , Fernandes Virgínia Cruz , Figueiredo Sónia A. , Delerue-Matos Cristina
Published: September 2022

Microplastics (MP) are spread into all ecosystems and represent a threat to the equilibrium of the environment and human health, not only due to their intrinsic characteristics but also to their action as effective carriers of contaminants, such as pesticides, pharmaceuticals, polychlorinated biphenyls and polycyclic aromatic hydrocarbons. The pesticide α-endosulfan is persistent and spread in the environment. The MP are another possible way of dissemination to be considered in the fate of this pesticide. The adsorption dynamics of α-endosulfan by six different MP (low-density polyethylene—LDPE, polyethylene-co-vinyl acetate, unplasticized polyvinyl chloride, polyamide 6, polystyrene granule, polypropylene granule) with different sizes/shapes and chemical compositions were evaluated. The most critical situation was identified for the system LDPE (particle size < 300 μm). Equilibrium studies (48 h equilibrium time) were performed for distilled, tap and filtered river water. Based on the Langmuir model parameters, the highest maximum adsorption capacity was obtained for distilled water, followed by filtered river and tap waters (i.e., 366 ± 39, 247 ± 38, 157 ± 22 μg/g). The obtained results demonstrate the important role that microplastics may have in the fate and transport of pesticides and their potentially harmful effect on the environment, which requires further investigation.
Influence of Cooling Rate on Crystallization Behavior of Semi-Crystalline Polypropylene: Experiments and Mathematical Modeling
Authors: Hu Yeyuan , Liao Yang , Zheng Yanyan , Ikeda Kosuke , Okabe Ryoji , Wu Ruifen , Ozaki Ryota , Xu Jun , Xu Qingyan
Published: September 2022

As crystallization behavior has a great effect on the injection molding process, the flash differential scanning calorimetry (FSC) method was employed to study the influence of cooling rate on the crystallization behavior of a semi-crystalline polypropylene (PP). As the experimental results show, crystallization temperatures (onset crystallization temperature and maximum crystallization temperature) and crystallinity decrease as the cooling rate increases. In addition, the corresponding mathematical models were established to describe the relationship between the crystallization temperatures/crystallinity and the cooling rate. A revised Tait equation was also carried out based on the mathematical models.
Post-Cure Development of the Degree of Conversion and Mechanical Properties of Dual-Curing Resin Cements
Authors: Carek Andreja , Dukaric Ksenija , Miler Helena , Marovic Danijela , Tarle Zrinka , Par Matej
Published: September 2022

This study investigated the effect of different curing conditions on the degree of conversion and mechanical properties of contemporary dual-curing resin cements. The material specimens were either light-cured directly, light-cured through a 1-mm lithium disilicate glass-ceramic layer, or self-cured. The degree of conversion was measured in 0.1-mm films using Fourier-transform infrared spectroscopy 1 day, 7 days, and 28 days post-cure. Specimens used to study the flexural strength and modulus were prepared according to the ISO 4049 protocol, stored for 28 days post-cure, and subjected to accelerated aging by absolute ethanol immersion. The degree of conversion values ranged between 44.3–77.8%. Flexural strength varied between 11.4–111.1 MPa, while flexural modulus amounted to 0.7–5.5 GPa. The degree of conversion was significantly affected by material type, curing conditions, and post-cure time; however, variations in curing conditions were the least influential factor. A statistically significant effect of curing conditions on the degree of conversion was identified for only one of the five materials tested, whereas the flexural strength and modulus of all tested materials were significantly reduced in the experimental groups that were light-cured through a ceramic layer or self-cured. The effect size analysis showed that mechanical properties were most affected by the material type, while the differences in curing conditions were less influential. A comparison of the degree of conversion and mechanical properties indicated that different curing conditions may lead to significantly different flexural strength and modulus, which are not necessarily accompanied by identifiable variations in the degree of conversion.
Rheological Properties of Styrene-Butadiene-Styrene Asphalt Mastic Containing High Elastic Polymer and Snow Melting Salt
Authors: Cao Yangsen , Li Jiarong , Liu Zhuangzhuang , Li Xinzhou , Zhang Fan , Shan Baozeng
Published: September 2022

Sprinkled snow melting salt (SMS) exerts a snow melting effect and also has a negative impact on the asphalt pavement and the environment. Salt storage pavement technology can alleviate these two problems. However, non-alkaline SMSs may have the risk of affecting asphalt mastic properties and further affecting the mechanical properties of asphalt pavements. Therefore, the general properties and rheological properties of two styrene-butadiene-styrene-modified asphalts with and without high elastic polymer were studied after adding SMS. The asphalt mastic without a high elastic agent is defined as the SBS group, and the other group is the HEA group. Our results show that the HEA group shows a lower penetration and a higher softening point, ductility, and viscosity than the SBS group. The more the SMS, the more the reduction effect of the general performance. The elastic recovery of asphalt mastic decreases with the content of SMS. SMS has no obvious effect on the ratio of the viscous and elastic composition of asphalt mastic. The creep of asphalt mastic increases with the content of SMS. The high elastic polymer can significantly reduce the creep, and even the strain of HEA100 is smaller than that of SBS00. SMS increases the creep stiffness and reduces the creep rate at low temperature. Although SMS increases the potential of asphalt pavement to melt ice and snow, it also reduces the high-temperature rutting resistance and low-temperature crack resistance of asphalt mastic. Salt storage pavement materials can be used in combination with high elastic polymers to reduce the negative effects brought by SMSs.
Attenuation of Pseudomonas aeruginosa Quorum Sensing Virulence of Biofilm and Pyocyanin by mBTL-Loaded Calcium Alginate Nanoparticles
Authors: Eltayb Esra Kamal , Alqahtani Fulwah Yahya , Alkahtani Hamad M. , Alsarra Ibrahim A. , Alfaraj Rihaf , Aleanizy Fadilah Sfouq
Published: September 2022

Pseudomonas aeruginosa contributes to many chronic infections and has been found to be resistant to multiple antibiotics. Pseudomonas use a quorum sensing system (QS) to control biofilm establishment and virulence factors, and, thus, quorum sensing inhibitors (QSIs), such as meta-bromo-thiolactone (mBTL), are promising anti-infective agents. Accordingly, this study intended to investigate the antibacterial and anti-virulence activity of mBTL-loaded calcium alginate nanoparticles (CANPs) against Pseudomonas aeruginosa and different QS mutants. The results show that the mBTL-CANPs had higher antibacterial activity, which was made evident by decreases in all tested strains except the ∆lasR/∆rhlR double mutant, with MIC50 (0.5 mg/mL) of mBTL-CANPs compared with free mBTL at MIC50 (˃1 mg/mL). The biofilm formation of P. aeruginosa and some QS-deficient mutants were reduced in response to 0.5–0.125 mg/mL of mBTL-encapsulating CANPs. The pyocyanin production of the tested strains except ∆lasA and ∆rhlR decreased when challenged with 0.5 mg/mL of mBTL-loaded NPs. The subsequent characterization of the cytotoxic effect of these NPs on human lung epithelial cells (A549) and cystic fibrosis fibroblast cells (LL 29) demonstrated that synthesized NPs were cytocompatible at MIC50 in both cell lines and markedly reduced the cytotoxic effect observed with mBTL alone on these cells. The resulting formulation reduced the P. aeruginosa strains’ adhesion to A549 comparably with mBTL, suggesting their potential anti-adhesive effect. Given the virulence suppressing action, cytocompatibility, and enhanced anti-biofilm effect of mBTL-CANPs, and the advantage of alginate-based NPs as an antimicrobial delivery system these nanoparticles have great potential in the prophylaxis and treatment of infection caused by Pseudomonas aeruginosa.
Dentin Sialoprotein/Phosphophoryn (DSP/PP) as Bio-Inductive Materials for Direct Pulp Capping
Authors: Chuang Shu-Fen , Chen Yu-Hsuan , Ma Peter X. , Ritchie Helena H.
Published: September 2022

Conventional direct pulp capping, such as calcium hydroxide (Ca(OH)2) or silicate products, usually induces an inflammatory reaction to provoke pulp regeneration. Phosphophoryn (PP) and dentin sialoprotein (DSP), the two most abundant non-collagenous proteins in the dentin matrix, are responsible for dentin mineralization, pulp cell migration, and differentiation. Here we examined the PP and combined DSP/PP as bio-inductive pulp capping materials by in vitro and in vivo tests. Firstly, the effects of the PP dose on pulp cell migration and matrix protein expression were examined by an agarose bead test. Secondly, the role of recombinant DSP (recDSP) and recDSP/PP on stimulating DSP-PP transcript expression was examined by RT-PCR. DSPP mRNA was also knocked down by RNA interference (RNAi) to examine their functions on dentin matrix mineralization. Finally, we used ferret animal models to test PP and recDSP/PP acting as capping agents on in vivo pulp responses and reparative dentin formation. The result showed that intermediate-dose PP was the most effective to enhance cell migration and differentiation. RecDSP/PP strongly enhanced the DSP-PP transcript expression, while inhibition of DSPP mRNA expression by siRNAs partially or completely affected dental pulp cell mineralization. The in vivo results showed that intermediate-dose PP and recDSP/PP proteins induced less pulp inflammation and promoted reparative dentin formation. Contrarily, conventional calcium hydroxide induced severe pulp inflammation. With these findings, DSP and PP could serve as capping agents for pulp capping therapy.
Organic–Inorganic Modification of Magnesium Borate Rod by Layered Double Hydroxide and 3-Aminopropyltriethoxysilane and Its Effect on the Properties of Epoxy Resin
Authors: Zou Sai , Dang Li , Li Ping , Zhu Jiachen , Lan Shengjie , Zhu Donghai
Published: September 2022

To alleviate the safety hazards associated with the use of epoxy resin (EP), a multifunctional filler was designed. This study firstly combines the superior mechanical properties of magnesium borate rods (MBR) with the excellent smoke suppression and flame-retardant characteristics of layered double hydroxide (LDH). H2PO4− intercalated LDH (LDHP) was coated on the MBR surface to obtain inorganic composite particles MBR@LDHP. Subsequently, MBR@LDHP was modified with 3-aminopropyltriethoxysilane (APES) to obtain organic-inorganic composite particles MBR@LDHP-APES. Eventually, the hybrid particles were added to EP to prepare the composite materials. Thereafter, the morphology, composition, and structure of MBR@LDHP-APES were characterized utilizing scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD). The results indicated the successful preparation of MBR@LDHP-APES, after which we investigated the effects of MBR@LDHP-APES on the smoke suppression, flame retardancy, and mechanical characteristics of EP. As observed, the EP composites containing 7.5 wt% MBR@LDHP-APES exhibited superior smoke suppression and flame retardancy abilities. The limiting oxygen index reached 33.5%, which is 36.73% greater than pure EP, and the lowest values of total heat and smoke release were observed for the composite materials. In addition, the mechanical properties test revealed that MBR@LDHP-APES considerably enhanced the tensile strength as well as the flexural strength of the composites. Furthermore, mechanistic studies suggested that the barrier effect of MBR, endothermic decomposition of LDHP, and the synergistic effect of LDHP and APES contributed essentially to the smoke suppression and flame-retardant properties of the material. The findings of this research point to a potential method for enhancing the EP’s ability to suppress smoke and flames while enhancing its mechanical properties.
Gender and its role in critical thinking and critical thinking strategies used by EFL learners
Authors: Azizi Mahmoud , Fallah-Zivlaee Shima , Králik Roman , Miština Juraj
Published: September 2022

Among many aspects of reading that were under investigation rightfully and thoroughly, the effect of gender on critical thinking in general and critical thinking strategy use, in particular, seemed to be neglected. The aim of this study is to investigate the possible role of gender in the critical thinking and critical thinking strategy use of EFL learners. To do so, a qualitative case study consisting of four participants was conducted. Two of them were male and the other two were female students at the University of Mazandaran. Analysis of participants' recorded think-aloud protocols reveals that first, gender had no role to play in the critical thinking and critical thinking strategy use; Second, both male and female participants of the study used the same variety of strategies. In this way, there was no difference in male and female strategy use and nothing particular emerged as feminine strategies.
Clean Living Culture through Online Learning Using Digital Media for Junior High School Students
Authors:   Universitas Pendidikan Indonesia, Indonesia, Br. Sinaga Desi Arita Maryani , Nandiyanto Asep Bayu Dani
Published: September 2022

The purpose of this study was to analyse the teaching of clean and healthy living culture in daily life through online learning using digital media. This learning uses Zoom meetings, google forms and the Wordwall site and provides pretest and posttest before and after learning to 22 junior high school students in Dayun District. Learning begins by introducing a clean and healthy living culture and the use of digital media such as Zoom meetings, Google forms and the Wordwall site. Pretest and Posttest are given through Google Forms with 20 questions. The results of the pretest and posttest given, show on average that students can know well the culture of clean and healthy living in daily life by using digital media as much as 81.82 points, but in using digital media as a tool for learning for students less can be said. This is due to various obstacles faced by students including, students rarely interact with teachers via zoom and only give homework through Google Classroom and WhatsApp, there are network problems or signals that occur when online learning takes place and lack of knowledge of digital media used such as laptop or cell phone. In this case, the introduction to sites such as Wordwall is also very minimal, so it requires a lot of interaction in using the site. This research can provide new information about learning the culture of clean and healthy living through online learning using digital media and shows the obstacles in using it
Application-Based CRM Relationship Implementation for Micro, Small and Medium Enterprises
Authors:   Departemen Sistem Informasi, Universitas Komputer Indonesia, Indonesia, Kusuma B S , Ramdhani I F , Azhara Z
Published: September 2022

This study aims to maintain good relations with consumers so that consumers do not turn to other competitors. The method used is the prototype method, which uses an approach to create a program quickly, gradually, making the information system better and easier. By using Customer Relationship Management (CRM) it can be useful for sales so that they can have good relations with consumers and so that consumers do not turn to others.
Social Media Affect on Contemporary Design for Architect and Client via Instagram Sites
Authors:   Departemen Teknik Arsitektur, Universitas Komputer Indonesia, Indonesia, Kosasih Imam Baihaqi , Sangaras Muhammad Abdul Gahar
Published: September 2022

The development of information technology, especially social media, not only has an impact on changing the behavior of its users, but also brings changes in various fields. The high intensity of human activity on social media can no doubt change the lifestyle of its users, including influencing the design of their dwellings. This phenomenon is expected to be used especially for architects to open up new opportunities in getting their clients. This study uses a qualitative method with a library research approach. This study displays scientific reasoning related to the development of social media which can have an impact on changes in work process patterns and the final result of a contemporary design work in the world of architecture. Like a client's request who plans a contemporary residential design following trends on social media, one of which is Instagram. Instagram is the third most used social media platform in the world. This is very effective for architects and prospective clients by utilizing social media to see references to the design of the building they want to plan. It is certain that the rapid development of social media information technology, especially Instagram, can affect the world of residential design.
The Application of Financial Recording Applications Towards Financial Report for Micro, Small and Medium Enterprises
Authors:   Departemen Akuntansi, Universitas Komputer Indonesia, Indonesia, Elmanda Finna Afrilia , Merdikawati Gusnerlisa Gita , Wahyuni Ratih
Published: September 2022

In this current era, many Micro, Small and Medium Enterprises (MSMEs) have started to use financial report recording applications in recording incoming and outgoing financial reports. This research aims to determine how much application of financial statement recording applications in making financial reports on Micro, Small and Medium Enterprises (MSMEs) using an android based financial recording application. This study uses descriptive quantitative research methods using data collection techniques in the form of questionnaires. The results of this study explain that the role of using applications to record financial reports for Micro, Small and Medium Enterprises has a positive impact on the sustainability of a business. By using an android-based financial recording application, it can make it easier to record financial reports and minimize errors in recording. Therefore, this research benefits Micro, Small and Medium Enterprises actors to understand better and understand technology so that they can apply financial statement recording applications properly.
Particularidades da histeroscopia na infertilidade - um advento diagnóstico
Authors: Leite Humberto Faria , Bento Ana Letícia Rossetti
Published: September 2022

Os distúrbios reprodutivos são uma afecção comum e afeta entre 10-15% dos casais ao redor do mundo. Por definição, tem-se como infertilidade feminina a incapacidade de estabelecer uma gravidez clínica com um parceiro saudável após um ano ou mais de relações sexuais regulares desprotegidas. Nesse cenário, a infertilidade está relacionada a diversas condições que influem e interferem na capacidade reprodutiva, tais como: anormalidades genéticas, endócrinas, fisiológicas, imunológicas e anatômicas do sistema reprodutor. Esta última, em especial, será abordada de maneira mais aprofundada no presente artigo. Estudos aventam que anormalidades localizadas na cavidade uterina, como pólipos, miomas, septos e/ou aderências, são as alterações anatômicas mais frequentes e guardam íntima relação com diversos casos de infertilidade. Quanto às modalidades diagnósticas de tais distúrbios anatômicos, a histeroscopia é considerada o método padrão ouro para a triagem em mulheres que possuem infertilidade inexplicada, propiciando a visualização e o manejo de fatores intrauterinos relacionados à infertilidade. Esse exame ginecológico é pautado na averiguação direta da cavidade uterina através de um endoscópio rígido, semi-rígido ou flexível, permitindo a análise das alterações através de imagens de altíssima qualidade. Desse modo, a histeroscopia diagnóstica pode auxiliar no reconhecimento precoce de afecções que são incompatíveis com a concepção. Além de ser um método diagnóstico, quando oportuno, a histeroscopia permite a remoção das anormalidades anatômicas, elevando as chances de fecundação da paciente.
Considerações acerca do Aglomerado Metropolitano de Curitiba na perspectiva da cidade - região Global
Authors: Alves Alceli Ribeiro
Published: September 2022

O objetivo desse trabalho consiste em analisar criticamente a hipótese de que o Aglomerado Metropolitano de Curitiba (AMC) representa uma escala de análise característica das cidades mundiais ou globais e que, portanto, possuem grande importância econômica e política no cenário mundial. Dentre algumas dessas cidades mundiais já figuraram em debates anteriores as cidades de Londres, Nova York e Tóquio, mas também Los Angeles e São Paulo. Utilizando o repertório teórico-conceitual da Cidade-Região Global, formulada por SCOTT et al., (2001) e uma variedade de dados quantitativos extraídos de diferentes bancos de dados, o trabalho obteve resultados que nos permitem afirmar o aumento da importância da escala do AMC em relação a Região Metropolitana de Curitiba (RMC). Contudo, as evidências obtidas nos permitem avaliar que o AMC, apesar de representar uma escala de análise relevante para a implantação de políticas públicas futuras, não nos permite rotulá-la como um recorte espacial de importância mundial tipificada comumente na literatura das cidades-regiões globais. Não obstante, o trabalho aponta também para a necessidade estendermos essas discussões e levantarmos novos questionamentos e debates no sentido de investigarmos outros temas pouco explorados nesta ocasião, tais como os políticos e sociais vinculados às temáticas aqui propostas.
Is there a relationship between the expression of β-catenin and Ki-67 in canine melanocytic neoplasms?
Authors: Machado Tanise Policarpo , Stolfo Josiane Borges , Costa Márcio Machado , Rodriguez Rubens , Da Motta Adriana Costa
Published: September 2022

An immunohistochemical evaluation of 26 cutaneous and oral benign and malignant canine melanocytic neoplasms was performed to identify a possible relationship between the expression of the β-catenin molecule with cell proliferation using Ki-67 expression. This molecule is a component of the Wnt/β-catenin signalling pathway, which causes a cascade of intracellular events that activate transduction genes and nuclear transcription. The microscopic evaluation was performed considering the β-catenin labelling site (cytoplasmic, nuclear or mixed). The mitotic index was evaluated by the expression of Ki-67 in 10 high power fields (HPF) (400x). Statistically significant difference was neither detected between the β-catenin labelling sites and the various neoplasms, nor a correlation between the β-catenin molecule and the cell proliferation marker Ki-67 in the oral or cutaneous, benign or malignant neoplasms. Our study brings interesting findings and points to future research on this topic, especially with established variables in the clinical, pathological and immunohistochemical fields.
Pedagogia para os bens comuns da humanidade no pré-escolar
Authors: Rocha Maria João Chainho Ataíde , Silva Ana Paula Lopes da
Published: September 2022

Conscientes das extremas assimetrias à escala mundial, o debate público, participativo e informado torna-se imperativo, no estabelecimento de orientações que contribuam para os bens comuns globais da Humanidade de forma construtiva, em todas os campos da ação humana, incluindo o campo estrito da pedagogia, com o reconhecimento de que “(…) what is learned is so important as the way it is taught” (Teodoro 2020, p.85). A suprema importância da educação no debate contra a apatia e alienação perante os fenómenos de exclusão social, desigualdades, violência representam a possibilidade de uma crescente edificação de políticas emancipadoras para uma mudança social. Acreditamos que com e através da educação se podem potenciar efetivas oportunidades de projetos que dignifiquem a condição humana, potenciando a afirmação de uma justiça social humanizadora. Bem como a relevância de evidenciarmos pontes de concertação internacional para que os direitos humanos se tornem de facto uma realidade. Assim, propomo-nos apresentar o nosso projeto de doutoramento que se focaliza no escutar e ler, de forma a interpretar as vozes das crianças sobre as representações que evidenciam nos seus quotidianos num contexto de educação pré-escolar. Um estudo que objetiva centrar-se na observação de um determinado contexto de educação pré-escolar, intentando responder à questão: Como é que as vozes e a participação das crianças são (des)consideradas no contexto educativo? Baseados em autores de referência no domínio da nossa temática, onde a pedagogia da escuta e da participação se afirma. Num paradigma de investigação interpretativo, através de um estudo de caso, etnográfico, com recurso à observação participante, objetivamos identificar a forma como a participação e as vozes das crianças emergem nos seus quotidianos de sala; analisar a forma como as crianças descrevem e interpretam as realidades e os factos do seu quotidiano de sala; e verificar como a participação e as vozes das crianças influenciam a gestão do processo educativo, pelas educadoras. Esperamos, assim, contribuir para a rutura da imagem da criança passiva, invisível e incompetente, sem direito de participação ativa nas decisões respeitantes às suas experiências educativas.
Preconceito, desconhecimento e problemas de implementação: uma revisão da literatura sobre políticas públicas para pessoas em situação de rua
Authors: Dalmolin Alice Gibeke Siqueira , Aguiar Rafael Barbosa de , Lima Luciana Leite
Published: September 2022

Devido ao grande contingente de pessoas em situação de rua no Brasil, em 2009 foi criada a Política Nacional para a População em Situação de Rua (PNPSR). De maneira intersetorial, tal política tem como objetivo assegurar o acesso das pessoas em situação de rua aos serviços ofertados pelo Estado. De forma complementar, em 2011, através da Portaria nº 2.488, foi criada estratégia específica voltada à saúde dessa população, o Programa Consultório na Rua (PCR). Nesse contexto de enfrentamento da problemática por meio de políticas públicas e da relativa novidade dessas iniciativas, o objetivo deste artigo é mapear a literatura científica sobre a PNPSR e o PCR, buscando identificar e sistematizar seus principais achados, os temas mais e menos abordados e como essas políticas têm sido implementadas. Para isso, realizou-se uma revisão sistemática da literatura, a coleta de dados foi realizada em março de 2022 nas bases de dados Capes, SciELO e Google Scholar, e foram identificados oito artigos. Constatou-se que a maioria das publicações tratavam das dificuldades de adesão ao PCR e dos seus problemas de implementação, que produzem barreiras de acesso à população em situação de rua.
Detecção de corrosão de aço carbono revestida com tinta industrial aditivada com Óxido de Grafeno por termografia ativa
Authors: Pinto Caio de Oliveira , Magalhães Olívia Karollyne Fidelix , Do Nascimento Bruna Figueiredo , Cavalcanti Jorge Vinícius Fernandes Lima , da Silva Ivan Costa , Farias Cláudia Teresa Teles , Cavalcanti Luiz Antonio Pimentel
Published: September 2022

A corrosão é um problema de escala global e detectá-la de maneira eficiente representa um grande avanço para o setor industrial. Algumas técnicas não destrutivas (END’s) possibilitam a detecção de processos corrosivos. A termografia infravermelho, técnica que se baseia no registro térmico da superfície do objeto, é amplamente utilizada nesse setor, possuindo rapidez, reprodutibilidade e baixo custo, mas com limitação de detecção a temperatura superficial do material, interferências de condições ambientais, e falta de precisão no acúmulo de calor nas descontinuidades. Nesse contexto, o presente estudo teve como objetivo avaliar a melhoria na detecção da corrosão por termografia ativa em corpos de prova de aço carbono SAE 1020, revestidos com pintura industrial aditivada com carga nanométrica de óxido de grafeno (OG). Foi sintetizado OG através do método de Hummers adaptado, sendo então designado para formulação da tinta de acabamento aditivada nas proporções de 0.1, 0.5, 1 e 2% (m/m). O corpo de provas foi pintado com duas demãos de tinta de base e uma de tinta de acabamento. Foram realizados ensaios de termografia ativa com intervalos de 3s, 6s e 9s, visando avaliar o período de tempo mais adequado para execução do ensaio. Como resultados, a utilização da tinta aditivada de OG auxiliou no sentido de maximizar a dispersão do calor pela superfície do corpo de prova, gerando imagens com menor interferências e possibilidade de dimensionamento mais preciso, os melhores resultados experimentais foram para o tempo de 6s e concentração de 0,5% de OG na tinta de acabamento.
Deep Learning in Cell Image Analysis
Authors: Xu Junde , Zhou Donghao , Deng Danruo , Li Jingpeng , Chen Cheng , Liao Xiangyun , Chen Guangyong , Heng Pheng Ann
Published: September 2022

Cell images, which have been widely used in biomedical research and drug discovery, contain a great deal of valuable information that encodes how cells respond to external stimuli and intentional perturbations. Meanwhile, to discover rarer phenotypes, cell imaging is frequently performed in a high-content manner. Consequently, the manual interpretation of cell images becomes extremely inefficient. Fortunately, with the advancement of deep-learning technologies, an increasing number of deep learning-based algorithms have been developed to automate and streamline this process. In this study, we present an in-depth survey of the three most critical tasks in cell image analysis: segmentation, tracking, and classification. Despite the impressive score, the challenge still remains: most of the algorithms only verify the performance in their customized settings, causing a performance gap between academic research and practical application. Thus, we also review more advanced machine learning technologies, aiming to make deep learning-based methods more useful and eventually promote the application of deep-learning algorithms.
The Role of Trust in Forming a Social Innovation Service System
Authors: Nguyen Thao , Pham Vy T.
Published: September 2022

The promotion of social innovation requires the interaction of multiple actors in a network who exchange resources to create value for customers. Adopting the Service-Dominant logic perspective, this paper explores how trust influences the emergence and evolvement of a social innovation service system. Data from a case study of One4One reveals that trust guides the activities of the social impact business and other actors in the service system to search for collaborators in a network, to lead the exchange of resources in the system for co-value creation, and to deal with other actors through the evolvement of the system. The study's findings could inform practitioners’ practices conducive to creating a social innovation service system that aims to improve the quality of life for people in developing countries like Vietnam.
How to Increase the Efficiency of Social Enterprises in Russia During the COVID-19 Pandemic?
Authors: Pavlov Ruslan
Published: September 2022

The paper considers the examples of economic activities of social enterprises during the COVID-19 pandemic. It is shown that often social enterprises are more effective than common enterprises in their responses to the processes caused by the COVID-19 pandemic. A model of interaction between different actors enhancing the efficiency of social enterprises in Russia by means of providing the continuous source of cashfrow is provided. At the macro-level of this model it’s necessary to mention such institutions as the Ministry of Finance, because it is engaged in planning and controlling the process of public procurement activities, The Public Relations Committee, as it provides subsidies to nonprofit organizations and social enterprises based on the competition rules and the Public Chamber, as it is engaged in distributing the Presidential Grants to nonprofit organizations and social enterprises. The institutions that provide financial help to social enterprises in the range of 1 to 50 million rubles belong to the meso-level institutions in this model. They consist of the Moscow regional division of the All-Russian Popular Front, the charitable foundation KAF, the fund “Our Future” and the company Unilever. At the micro-level of this model there are two institutions: Impact Hub Moscow and Awesome Foundation. A contact should be set between these organizations to exchange the experience in the area of supporting social entrepreneurs and attracting the micro-grants of Awesome Foundation as the supplementary sources of support for the winners of the competition held by Impact Hub Moscow. This system should be balanced by setting direct contacts between each institution at the respective level so that it could enable these institutions to act effectively at each level of that model. First, we should conclude that these institutions should not act as isolated units. They should be implemented into the system of different actors supporting social enterprises, along with such potential investors as private sector companies and microfinance institutions. Second, these actors should interact in such manner that enables a cooperation between them. Conclusions refer to how these institutions should be arranged to make a system of interconnected units supporting social enterprises at three levels.