bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Machine Learning (ML)-Based Classification Using Conventional 18F-FDG PET Parameters in Predicting Postsurgical Features of Endometrial Cancer Aggressiveness
Authors: Bezzi Carolina , Bergamini Alice , Mathoux Gregory , Ghezzo Samuele , Monaco Lavinia , Candotti Giorgio , Fallanca Federico , Gajate Ana Maria Samanes , Rabaiotti Emanuela , Cioffi Raffaella , Bocciolone Luca , Gianolli Luigi , Taccagni GianLuca , Candiani Massimo , Mangili Giorgia , Mapelli Paola , Picchio Maria
Published: January 2023

Purpose: to investigate the preoperative role of ML-based classification using conventional 18F-FDG PET parameters and clinical data in predicting features of EC aggressiveness. Methods: retrospective study, including 123 EC patients who underwent 18F-FDG PET (2009–2021) for preoperative staging. Maximum standardized uptake value (SUVmax), SUVmean, metabolic tumour volume (MTV), and total lesion glycolysis (TLG) were computed on the primary tumour. Age and BMI were collected. Histotype, myometrial invasion (MI), risk group, lymph-nodal involvement (LN), and p53 expression were retrieved from histology. The population was split into a train and a validation set (80–20%). The train set was used to select relevant parameters (Mann-Whitney U test; ROC analysis) and implement ML models, while the validation set was used to test prediction abilities. Results: on the validation set, the best accuracies obtained with individual parameters and ML were: 61% (TLG) and 87% (ML) for MI; 71% (SUVmax) and 79% (ML) for risk groups; 72% (TLG) and 83% (ML) for LN; 45% (SUVmax; SUVmean) and 73% (ML) for p53 expression. Conclusions: ML-based classification using conventional 18F-FDG PET parameters and clinical data demonstrated ability to characterize the investigated features of EC aggressiveness, providing a non-invasive way to support preoperative stratification of EC patients.
Early Identification of Pneumonitis in Patients Irradiated for Lung Cancer—Final Results of the PARALUC Trial
Authors: Rades Dirk , Werner Elisa M. , Glatzel Esther , Bohnet Sabine , Schild Steven E. , Tvilsted Søren S. , Janssen Stefan
Published: January 2023

Radiotherapy of lung cancer may cause pneumonitis that generally occurs weeks or months following therapy and can be missed. This prospective trial aimed to pave the way for a mobile application (app) allowing early diagnosis of pneumonitis. The primary goal was the identification of the optimal cut-off of a score to detect pneumonitis of grade ≥2 after radiotherapy for lung cancer. Based on the severity of symptoms (cough, dyspnea, fever), scoring points were 0–9. Receiver operating characteristic (ROC)-curves were used to describe the sensitivity and specificity. The area under the ROC-curve (AUC) was calculated to judge the accuracy of the score, Youden-index was employed to define the optimal cut-off. Until trial termination, 57 of 98 patients were included. Eight of 42 patients evaluable for the primary endpoint (presence or absence of radiation pneumonitis) experienced pneumonitis. AUC was 0.987 (0.961–1.000). The highest sensitivity was achieved with 0–4 points (100%), followed by 5 points (87.5%), highest specificity with 5–6 points (100%). The highest Youden-index was found for 5 points (87.5%). The rate of patient satisfaction with the symptom-based scoring system was 93.5%. A cut-off of 5 points was identified as optimal to differentiate between pneumonitis and no pneumonitis. Moreover, pneumonitis was significantly associated with an increase of ≥3 points from baseline (p < 0.0001). The scoring system provided excellent accuracy and high patient satisfaction. Important foundations for the development of a mobile application were laid.
Deep Learning for Detecting Brain Metastases on MRI: A Systematic Review and Meta-Analysis
Authors: Ozkara Burak B. , Chen Melissa M. , Federau Christian , Karabacak Mert , Briere Tina M. , Li Jing , Wintermark Max
Published: January 2023

Since manual detection of brain metastases (BMs) is time consuming, studies have been conducted to automate this process using deep learning. The purpose of this study was to conduct a systematic review and meta-analysis of the performance of deep learning models that use magnetic resonance imaging (MRI) to detect BMs in cancer patients. A systematic search of MEDLINE, EMBASE, and Web of Science was conducted until 30 September 2022. Inclusion criteria were: patients with BMs; deep learning using MRI images was applied to detect the BMs; sufficient data were present in terms of detective performance; original research articles. Exclusion criteria were: reviews, letters, guidelines, editorials, or errata; case reports or series with less than 20 patients; studies with overlapping cohorts; insufficient data in terms of detective performance; machine learning was used to detect BMs; articles not written in English. Quality Assessment of Diagnostic Accuracy Studies-2 and Checklist for Artificial Intelligence in Medical Imaging was used to assess the quality. Finally, 24 eligible studies were identified for the quantitative analysis. The pooled proportion of patient-wise and lesion-wise detectability was 89%. Articles should adhere to the checklists more strictly. Deep learning algorithms effectively detect BMs. Pooled analysis of false positive rates could not be estimated due to reporting differences.
Theoretical and Cyclic Voltammetric Analysis of Asparagine and Glutamine Electrocatalytic Activities for Dopamine Sensing Applications
Authors: Kudur Jayaprakash Gururaj , Swamy B. E. Kumara , Flores-Moreno Roberto , Pineda-Urbina Kayim
Published: January 2023

The molecular dynamics and density functional theory (DFT) can be applied to discriminate electrocatalyst’s electron transfer (ET) properties. It will be interesting to discriminate the ET properties of green electrocatalysts such as amino acids. Here, we have used DFT to compare the electrocatalytic abilities of asparagine and glutamine at the carbon paste electrode interface. Cyclic voltammetric results reveal that the electrocatalytic activities of aspargine are higher than glutamine for dopamine sensing. Dopamine requires less energy to bind with asparagine when compared to glutamine. Additionally, asparagine has higher electron-donating and accepting powers. Therefore, asparagine has a higher electrocatalytic activity than glutamine—the ability for the asparagine and glutamine carbon electrodes to detect dopamine in commercial injection, and to obtain satisfactory results. As a part of the work, we have also studied dopamine interaction with the modified carbon surface using molecular dynamics.
Selective Furfuryl Alcohol Production from Furfural via Bio-Electrocatalysis
Authors: Zhan Peng , Liu Xiangshi , Zhu Qian , Zhao Hongqing , Zhang Shiding , Zhang Chenxi , Ren Cong , Zhang Jiawen , Zhang Changwei , Cai Di
Published: January 2023

The catalytic reduction of renewable furfural into furfuryl alcohol for various applications is in the ascendant. Nonetheless, the conventional chemo-catalysis hydrogenation of furfural always suffers from poor selectivity, harsh conditions, and expensive catalysts. Herein, to overcome the serious technical barriers of conventional furfuryl alcohol production, an alternative bio-electrocatalytic hydrogenation system was established under mild and neutral conditions, where the dissolved cofactor (NADH) and the alcohol dehydrogenase (ADH) participated in a tandem reaction driven by the electron from a novel Rh (III) complex fixed cathode. Under the optimized conditions, 81.5% of furfural alcohol selectivity can be realized at −0.43 V vs. RHE. This contribution presents a ‘green’ and promising route for the valorization of furfural and other biomass compounds.
Production of Biomodified Bleached Kraft Pulp by Catalytic Conversion Using Penicillium verruculosum Enzymes: Composition, Properties, Structure, and Application
Authors: Shevchenko Aleksandr R. , Tyshkunova Irina V. , Chukhchin Dmitry G. , Malkov Alexey V. , Toptunov Evgeniy A. , Telitsin Vadim D. , Rozhkova Aleksandra M. , Sinitsyna Olga A. , Gofman Iosif V. , Aksenov Andrey S.
Published: January 2023

The global development of the bioeconomy is impossible without technologies for comprehensive processing of plant renewable resources. The use of proven pretreatment technologies raises the possibility of the industrial implementation of the enzymatic conversion of polysaccharides from lignocellulose considering the process’s complexity. For instance, a well-tuned kraft pulping produces a substrate easily degraded by cellulases and hemicelulases. Enzymatic hydrolysis of bleached hardwood kraft pulp was carried out using an enzyme complex of endoglucanases, cellobiohydrolases, β-glucosidases, and xylanases produced by recombinant strains of Penicillium verruculosum at a 10 FPU/g mixture rate and a 10% substrate concentration. As a result of biocatalysis, the following products were obtained: sugar solution, mainly glucose, xylobiose, xylose, as well as other minor reducing sugars; a modified complex based on cellulose and xylan. The composition of the biomodified kraft pulp was determined by HPLC. The method for determining the crystallinity on an X-ray diffractometer was used to characterize the properties. The article shows the possibility of producing biomodified cellulose cryogels by amorphization with concentrated 85% H3PO4 followed by precipitation with water and supercritical drying. The analysis of the enzymatic hydrolysate composition revealed the predominance of glucose (55–67%) among the reducing sugars with a maximum content in the solution up to 6% after 72 h. The properties and structure of the modified kraft pulp were shown to change during biocatalysis; in particular, the crystallinity increased by 5% after 3 h of enzymatic hydrolysis. We obtained cryogels based on the initial and biomodified kraft pulp with conversion rates of 35, 50, and 70%. The properties of these cryogels are not inferior to those of cryogels based on industrial microcrystalline cellulose, as confirmed by the specific surface area, degree of swelling, porosity, and SEM images. Thus, kraft pulp enzymatic hydrolysis offers prospects not only for producing sugar-rich hydrolysates for microbiological synthesis, but also cellulose powders and cryogels with specified properties.
Graphene-Based Derivatives Heterostructured Catalytic Systems for Sustainable Hydrogen Energy via Overall Water Splitting
Authors: Sadiq Iqra , Ali Syed Asim , Ahmad Tokeer
Published: January 2023

The global climate crisis has cultivated the demand for sustainable energy resources as fossil derivative fuels are functional in catalyzing the rate of environmental breakdown. Sustainable energy solutions generate various renewable energy prospects capable of delivering efficient energy operations. Among these prospects, green H2 energy generated via overall water splitting is an effective approach towards sustainability ascribed to the higher gravimetric density and efficiency of H2 fuel. In this review, we sought to discuss the applicability and challenges of graphene-based derivatives in H2 evolution operations through photochemical, electrochemical and photoelectrochemical water-splitting pathways. The unique layered structure of graphene-based derivatives alongside marvelous optoelectronic and physicochemical properties ease out the thermodynamic uphill of water splitting better than their non-layered counterparts. In addition, the heterojunction formation in the graphene derivatives with visible light catalysts propels the kinetics of HER. Functionalized GO and rGO derivatives of graphene are riveting catalysts that have received extensive interest from researchers attributed to their accelerated chemical and mechanical stability, tunable band structure and larger surface area, providing more exposed active sites for HER. The surface organic functional groups of GO/rGO assist in establishing synergetic interfacial contact with other catalysts. Thus, these groups provide structural and chemical versatility to GO/rGO-based heterostructured catalysts, which effectively improve their physicochemical parameters that drive their catalytic performance towards HER. In order to develop a cost-effective and highly efficient catalytic system, graphene-based derivatives are promising heterostructured catalysts that exhibit a good relationship between catalytic efficiency and robustness.
CuO/ZnO/CQDs@PAN Nanocomposites with Ternary Heterostructures for Enhancing Photocatalytic Performance
Authors: Xu Huanhuan , Fan Peizhi , Xu Lan
Published: January 2023

Photocatalysis is a green technology. In this paper, CuO/ZnO/carbon quantum dots (CQDs)@PAN nanocomposites with ternary heterostructures (CZC@PAN)—as high-performance environmentally friendly nanophotocatalysts—were prepared by electrospinning, heat treatment, and hydrothermal synthesis in sequence, and their practical applications were investigated by degrading methylene blue (MB). The synergistic effects of components in ternary heterostructures on the morphology, structure, and photocatalytic performance of CZC@PAN were analyzed, and their photocatalytic mechanism was further discussed. The results showed that due to the formation of p-n heterojunctions and the loading of CQDs and CZC@PAN had excellent photocatalytic degradation performance, and its photocatalytic degradation rate for MB reached 99.56% under natural sunlight for 4 h.
Morphological and Functional Effects of Ultrasound on Blood–Brain Barrier Transitory Opening: An In Vitro Study on Rat Brain Endothelial Cells
Authors: Branca Jacopo Junio Valerio , Boninsegna Matteo , Morucci Gabriele , Carrino Donatello , Nicoletti Claudio , Paternostro Ferdinando , Gulisano Massimo , Bocchi Leonardo , Pacini Alessandra
Published: January 2023

With the recent advances in medicine, human life expectancy is increasing; however, the extra years of life are not necessarily spent in good health or free from disability, resulting in a significantly higher incidence of age-associated pathologies. Among these disorders, neurodegenerative diseases have a significant impact. To this end, the presence of the protective blood–brain barrier (BBB) represents a formidable obstacle to the delivery of therapeutics. Thus, this makes it imperative to define strategies to bypass the BBB in order to successfully target the brain with the appropriate drugs. It has been demonstrated that targeting the BBB by ultrasound (US) can transiently make this anatomical barrier permeable and in so doing, allow the delivery of therapeutics. Thus, our aim was to carry out an in depth in vitro molecular and morphological study on the effects of US treatment on the BBB. The rat brain endothelial (RBE4) cell line was challenged with exposure to 12 MHz diagnostic US treatment for 10, 20, and 30 min. Cell viability assays, Western blotting analysis on the endoplasmic reticulum (ER), and oxidative stress marker evaluation were then performed, along with cytological and immunofluorescence staining, in order to evaluate the effects of US on the intercellular spaces and tight junction distribution of the brain endothelial cells. We observed that the US treatment exerted no toxic effects on either RBE4 cell viability or the upregulation/dislocation of the ER and oxidative stress marker (GRP78 and cytochrome C, respectively). Further, we observed that the application of US induced an increase in the intercellular spaces, as shown by Papanicolaou staining, mainly due to the altered distribution of the tight junction protein zonula occludens-1 (ZO-1). This latter US-dependent effect was transient and disappeared 20 min after the removal of the stimulus. In conclusion, our results show that US induces a transient alteration of the BBB, without altering the intracellular signaling pathways such as the ER and oxidative stress that could potentially be toxic for endothelial cells. These results suggested that US treatment could represent a potential strategy for improving drug delivery to the brain.
Meta-Analysis of MS-Based Proteomics Studies Indicates Interferon Regulatory Factor 4 and Nucleobindin1 as Potential Prognostic and Drug Resistance Biomarkers in Diffuse Large B Cell Lymphoma
Authors: Ejtehadifar Mostafa , Zahedi Sara , Gameiro Paula , Cabeçadas José , da Silva Maria Gomes , Beck Hans C. , Carvalho Ana Sofia , Matthiesen Rune
Published: January 2023

The prognosis of diffuse large B cell lymphoma (DLBCL) is inaccurately predicted using clinical features and immunohistochemistry (IHC) algorithms. Nomination of a panel of molecules as the target for therapy and predicting prognosis in DLBCL is challenging because of the divergences in the results of molecular studies. Mass spectrometry (MS)-based proteomics in the clinic represents an analytical tool with the potential to improve DLBCL diagnosis and prognosis. Previous proteomics studies using MS-based proteomics identified a wide range of proteins. To achieve a consensus, we reviewed MS-based proteomics studies and extracted the most consistently significantly dysregulated proteins. These proteins were then further explored by analyzing data from other omics fields. Among all significantly regulated proteins, interferon regulatory factor 4 (IRF4) was identified as a potential target by proteomics, genomics, and IHC. Moreover, annexinA5 (ANXA5) and nucleobindin1 (NUCB1) were two of the most up-regulated proteins identified in MS studies. Functional enrichment analysis identified the light zone reactions of the germinal center (LZ-GC) together with cytoskeleton locomotion functions as enriched based on consistent, significantly dysregulated proteins. In this study, we suggest IRF4 and NUCB1 proteins as potential biomarkers that deserve further investigation in the field of DLBCL sub-classification and prognosis.
Sigma-1 Receptor as a Protective Factor for Diabetes-Associated Cognitive Dysfunction via Regulating Astrocytic Endoplasmic Reticulum-Mitochondrion Contact and Endoplasmic Reticulum Stress
Authors: Du Mengyu , Jiang Tao , He Shuxuan , Cheng Bo , Zhang Xin , Li Liya , Yang Lan , Gao Wei , Li Yansong , Wang Qiang
Published: January 2023

The prevalence of diabetes-associated cognitive dysfunction (DACD) has increased to 13.5%. Dementia, as the most severe DACD, is the second leading cause of death in patients with diabetes mellitus. Hence, the potential mechanisms of DACD for slowing or halting its progression need to be urgently explored. Given that the sigma-1 receptor (Sig-1R), a chaperone protein located in the endoplasmic reticulum (ER)-mitochondrion contact membranes to regulate ER stress (ERS), is associated with cognitive outcomes in neurodegenerative diseases, this study aimed to investigate the role of astrocytic Sig-1R in DACD and its underlying mechanism. Here, we examined the levels of ERS and complement component 3/3a (C3/C3a) from primary astrocytes with different concentrations of glucose and treatment. Subsequently, HT22 neurons were cultured in different astrocyte-conditioned medium, and the expression of synaptic proteins was detected. We constructed type 1 diabetes mellitus (T1DM) model to evaluate the astrocytic Sig-1R mechanism on synapse and cognitive function changes. In vitro, high glucose concentration downregulated Sig-1R and aggravated ERS in astrocytes, resulting in synapse deficits. PRE-084, a high-affinity and selective Sig-1R agonist, inhibited astrocytic ERS and complement cascades and restored synaptic damage, while the Sig-1R antagonist displayed the opposite results. Moreover, C3a receptor antagonist (C3aRA) could mimic the effect of PRE-084 and exerted neuroprotective effects. In vivo, PRE-084 substantially reduced ER-mitochondrion contact, activation of ERS, and C3/C3a secretion in mice with T1DM. Additionally, the synaptic loss and neurobehavioral dysfunction of mice with T1DM were less pronounced in both the PRE-084 and C3aRA treatment groups. These findings demonstrated that Sig-1R activation reduced the astrocytic ER-mitochondrion contact, ERS activation, and complement-mediated synaptic damage in T1DM. This study suggested the mechanisms and potential therapeutic approaches for treating DACD.
The Novel Inhibitory Effect of YM976 on Adipocyte Differentiation
Authors: Kim Hee Jung , Kim Dong-Hoon , Um Sung Hee
Published: January 2023

The pyrimidine derivative YM976 (4-(3-chlorophenyl)-1,7-diethylpyrido(2,3-d)-pyrimidin-2(1H)-one) exerts anti-inflammatory and anti-asthmatic effects. Considering that accumulation of lipids in adipose tissue is accompanied by inflammation, we investigated whether YM976 affects adipocyte differentiation. We found that YM976 significantly decreased lipid accumulation without cytotoxicity and reduced the expression levels of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) as well as their lipogenic regulators including fatty acid synthase (FASN) and fatty acid-binding protein 4 (FABP4) in 3T3-L1 cells induced for differentiation. YM976 mainly inhibited the early stage of adipocyte differentiation. Furthermore, intracellular cAMP level was elevated by YM976 resulting in increased phosphorylation of adenosine monophosphate-activated protein kinase (AMPK). Conversely, decreasing the levels of AMPK or treatment with Compound C, an AMPK inhibitor, lessened the suppressive effects of YM976 on PPARγ transcriptional activity and adipogenesis. Thus, our results suggest YM976 as a novel potential compound for controlling lipid accumulation and formation of adipocytes in obesity.
Role of Hepatocyte Growth Regulators in Liver Regeneration
Authors: Kimura Mitsutoshi , Moteki Hajime , Ogihara Masahiko
Published: January 2023

We have studied whether growth factors, cytokines, hormones, neurotransmitters, and local hormones (autacoids) promote the proliferation of hepatic parenchymal cells (i.e., hepatocytes) using in vitro primary cultured hepatocytes. The indicators used for this purpose include changes in DNA synthesis activity, nuclear number, cell number, cell cycle, and gene expression. In addition, the intracellular signaling pathways from the plasma membrane receptors to the nucleus have been examined in detail for representative growth-promoting factors that have been found to promote DNA synthesis and cell proliferation of hepatocytes. In examining intracellular signaling pathways, the effects of specific inhibitors of presumed signaling factors involved have been pharmacologically confirmed, and the phosphorylation activities of the signaling factors (e.g., RTK, ERK, mTOR, and p70 S6K) have been evaluated. As a result, it has been found that there are many factors that promote the proliferation of hepatocytes (e.g., HGF, EGF, TGF-α, IL-1β, TNF-α, insulin, growth hormone (GH), prostaglandin (PG)), and serotonin (5-HT)), while there are very few factors (e.g., TGF-β1 and glucocorticoids) that inhibit the effects of growth-promoting factors. We have also found that 5-HT and GH promote the proliferation of hepatocytes via different autocrine factors (e.g., TGF-α and IGF-I, respectively). Using primary cultured hepatocytes, it will be possible to further study the molecular and cellular aspects of liver regeneration.
Optical Technologies for Single-Cell Analysis on Microchips
Authors: Ou Xiaowen , Chen Peng , Liu Bi-Feng
Published: January 2023

Cell analysis at the single-cell level is of great importance to investigate the inherent heterogeneity of cell populations and to understand the morphology, composition, and function of individual cells. With the continuous innovation of analytical techniques and methods, single-cell analysis on microfluidic chip systems has been extensively applied for its precise single-cell manipulation and sensitive signal response integrated with various detection techniques, such as optical, electrical, and mass spectrometric analyses. In this review, we focus on the specific optical events in single-cell analysis on a microfluidic chip system. First, the four most commonly applied optical technologies, i.e., fluorescence, surface-enhanced Raman spectroscopy, surface plasmon resonance, and interferometry, are briefly introduced. Then, we focus on the recent applications of the abovementioned optical technologies integrated with a microfluidic chip system for single-cell analysis. Finally, future directions of optical technologies for single-cell analysis on microfluidic chip systems are predicted.
Plasma-Polymerized Thiophene-Reduced Graphene Oxide Composite Film Sensor for Ammonia/Amine Detection at Room Temperature
Authors: Nadekar Baliram , Khollam Yogesh B. , Shaikh Shoyebmohamad F. , Trimukhe Ajinkya , Deshmukh Rajendra , More Pravin S. , Siddiqui Muhammad Usman Hassan , Rana Abu ul Hassan S. , Palaniswami Marimuthu
Published: January 2023

Industrialization has led to an increasing need for specific and selective gas sensors in the past few decades. Environmental monitoring of certain volatile compounds such as ammonia is necessary. Advancements in the food storage sector have created the need for cheap and effective amine chemosensors. Classical chemosensors still face several issues, such as a lack of selectivity and low sensitivity toward ammonia and amines. Sensitivity is defined as the relative change in response expressed in percentage. In this work, we have resolved a few issues associated with the ammonia and amine sensors, such as low selectivity, long-term instability, and unreliability under higher temperatures using plasma-polymerized thiophene (PPTh) reduced graphene oxide (rGO) composite films. PPTh films were prepared using RF plasma polymerization with optimized deposition parameters. Several samples were evaluated for their sensing response to understand the optimal PPTh and rGO ratio in the PPTh-rGO composite. These composite PPTh-rGO films have shown 4 times higher sensitivity for ammonia/amines than individual PPTh and rGO films. Ammonia, methylamine (MA), dimethylamine (DMA), and trimethylamine (TMA) were primary analytes and tested for sensing response of the PPTh-rGO composite. The sensitivity measured ranges from 1328 for trimethylamine to 2354 for methylamine at 1000 ppm. The order of sensitivity was found to be MA > Ammonia > DMA > TMA. Polymer swelling, reduced charge carriers, and disruption of conductive pathways can explain possible sensing mechanisms. PPTh-rGO composite films have shown selectivity as high as 110 for ammonia/amine over other commonly used volatile organic compounds. The sensing response of these films is stable for any temperature fluctuations from 30 °C to 150 °C. Additionally, films showed stable sensitivity for over 4 months. Thus, composite films of PPTh-rGO can be effectively used to develop highly selective and stable gas sensors for the environmental monitoring of ammonia/amines.
Unusual ‘Turn-on’ Ratiometric Response of Fluorescent Porphyrin-Pyrene Dyads to the Nitroaromatic Compounds
Authors: Shepeleva Irina I. , Birin Kirill P. , Polivanovskaia Daria A. , Martynov Alexander G. , Shokurov Alexander V. , Tsivadze Aslan Yu. , Selektor Sofiya L. , Gorbunova Yulia G.
Published: January 2023

Detection of nitroaromatic compounds (NAC) is an important task since these substances are hazardous to both the biosphere and the society. Fluorescent sensors developed for NAC detection usually demonstrate a ‘turn-off’ response to the analyte, while ‘turn-on’ sensors are rarely reported. Here, we present a showcase report on new pyrene-imidazoporphyrin dyads that demonstrate an unusual analytic response to NAC with clear ‘turn-on’ behavior followed by an unexpected appearance of a new band, which can be ascribed to exciplex emission. The porphyrin backbone of the dyad also allows registration of its own fluorescence, providing an internal reference signal for ratiometric detection. The association constants in the order of 104 M−1 are reported.
Suspect Screening and Semi-Quantification of Macrolide Antibiotics in Municipal Wastewater by High-Performance Liquid Chromatography—Precursor Ion Scan Tandem Mass Spectrometry
Authors: Voronov Ilya S. , Falev Danil I. , Ul’yanovskii Nikolay V. , Kosyakov Dmitry S.
Published: January 2023

Macrolides are widely used in medicine and veterinary medicine, and are the leading antibiotics in terms of consumption. The release of macrolides and their metabolites into the environment through municipal wastewater can have an adverse effect on aquatic ecosystems and human health. In the present study, a method for the non-targeted screening and semi-quantitative determination of macrolide antibiotics and their derivatives in wastewater based on a combination of chromatographic separation and tandem mass spectrometric detection in precursor ion scan (PrecIS) mode has been proposed. Product ions with m/z 158 and 174 related to specific desosamine fragments were used as diagnostic ions for the PrecIS detection of the macrolide structures without (14- and 15-membered macrocycles) and with a (16-membered macrocycle) glycosylated desosamine moiety, respectively. The combination of the optimized solid phase extraction procedure and HPLC-MS/MS analysis in PrecIS mode allowed for the suspect screening of macrolides in municipal wastewater with limits of detection in the range of 4–150 ng L−1. The developed approach made it possible to detect and tentatively identify in municipal wastewater 17 compounds belonging to the macrolide class, including azithromycin, clarithromycin, josamycin and 14 metabolites with a total concentration of 1450 ng L−1.
Parental Perceptions of Child’s Play in the Post-Digital Era: Parents’ Dilemma with Digital Formats Informing the Kindergarten Curriculum
Authors: Istenič Andreja , Rosanda Violeta , Volk Marina , Gačnik Mateja
Published: January 2023

Digital technology affordance has been recognized as a social and learning tool, and the requirements for digitalizing the kindergarten curriculum have been present for decades. Digitalization in a child’s early years can present conflict with parents, as the societal and economic demands of digitalized society for a child’s digital technology use are in dissonance with guidelines and recommendations of health organizations that caution against preschool children’s technology use. Kindergarten curricular reform in Slovenia was conducted in the 1990s. In this period, the use of digital learning technology and digital play for the development of children learning predispositions and early literacy was already recognized. At the time of curriculum design, it integrated some elements of digital learning technology. Now, 30 years after the design of the new curriculum, we are facing the post-digital era. Learning technology in the early years is a matter of partnership with parents; accordingly, this descriptive survey study includes a non-randomized sample of 306 parents. We are considering how child’s play is structured in the primary environment and how parents perceive digital technology in the current post-digital age of seamless, digitally saturated social practices. Parents are aware of the risks of technology and of its benefits for learning. The findings show correlations between a child’s digital screen technology use and parents’ attitudes and perceptions of digital play. Parents that offer digital screen technology to a child have less agreeable attitudes regarding its possible risks to a child.
Early Life Stress (ELS) Effects on Fetal and Adult Bone Development
Authors: Pappalardo Xena , Testa Gianluca , Pellitteri Rosalia , Dell’Albani Paola , Rodolico Margherita , Pavone Vito , Parano Enrico
Published: January 2023

Early life stress (ELS) refers to harmful environmental events (i.e., poor maternal health, metabolic restraint, childhood trauma) occurring during the prenatal and/or postnatal period, which may cause the ‘epigenetic corruption’ of cellular and molecular signaling of mental and physical development. While the impact of ELS in a wide range of human diseases has been confirmed, the ELS susceptibility to bone diseases has been poorly explored. In this review, to understand the potential mediating pathways of ELS in bone diseases, PRISMA criteria were used to analyze different stress protocols in mammal models and the effects elicited in dams and their progeny. Data collected, despite the methodological heterogeneity, show that ELS interferes with fetal bone formation, also revealing that the stress type and affected developmental phase may influence the variety and severity of bone anomalies. Interestingly, these findings highlight the maternal and fetal ability to buffer stress, establishing a new role for the placenta in minimizing ELS perturbations. The functional link between ELS and bone impairments will boost future investigations on maternal stress transmission to the fetus and, parallelly, help the assessment of catch-up mechanisms of skeleton adaptations from the cascading ELS effects.
Safety of Vaccination within First Year of Life—The Experience of One General Medicine Center
Authors: Pop Claudia Felicia , Coblisan Petronela , Capalna Ligia , Panța Paraschiva Cherecheș , Buzoianu Anca Dana , Bocsan Ioana Corina
Published: January 2023

Vaccines represent an important preventive strategy in paediatric populations, but the rate of vaccination has been constantly declining in the last decade. Concerns about vaccines’ safety represent one of the main causes of vaccine hesitancy among parents. The aim of this study was the analysis of the immediate adverse reactions induced by vaccines included in the national program of immunization for the first year of life. Method: Eighty-one children born between 1st of January 2018 and 31st of March 2019 were included. The vaccination refusal rate, and incidence and severity of adverse effects induced by three mandatory vaccines (Hexavalent, Prevenar 13 and MMR) were analyzed. The level of parents’ education and the sources of information that were consulted in order to understand the adverse effects were also analyzed. Results and conclusions: The rate of adverse events was lower than 30% of the total number of doses, and most of them were mild. The incidence and severity of vaccine-induced adverse effects were correlated with prematurity. The vaccine hesitancy rate was lower than the national one, possibly due to a high level of education and good information provided by doctors that might have led to parents’ concerns regarding vaccination being answered.
Atmospheric Plasma Lingual Frenectomy Followed by Post Operative Tongue Exercises: A Case Series
Authors: Scarano Antonio , Di Giulio Rosanna , Gehrke Sergio Alexandre , Tagariello Gianluca , Romano Francesco , Lorusso Felice
Published: January 2023

The lingual frenulum is a submucosal component significantly involved in the mobility of the tongue. In the case of short lingual frenulum, different surgical approaches have been proposed. Atmospheric plasma is a thermal technique of vaporization or sublimation of the superficial tissues, without going deep, and the resulting fine carbonized layer avoids bleeding. The aim of the present investigation was to evaluate the effectiveness of atmospheric plasma (voltaic arc dermabrasion) for the frenectomy of a short lingual frenulum. A total of 30 patients with an age range between 6–11 years old and a class III/IV Kotlow’s ankyloglossia classification were included in the study. The Kotlow’s free-tongue, maximal interincisal mouth opening (MIO, mm) and interincisal mouth opening with tongue tip to maxillary incisive papillae at roof of mouth (MOTTIP, mm) were calculated at the baseline, immediately postoperatively (T0), at one week (T1), one month (T2) and two months (T3). A significant increase of Kotlow’s measurements, MOTTIP and MIO were detected when comparing the baseline and the T0 (p < 0.05). No significant difference was detected between the T0, T1 and T2 (p > 0.05). The atmospheric plasma demonstrated a very minimal invasive approach for frenectomy, without important or fibrotic complications and with very low recurrence rates.
Application of the Self-Made Flexible Three-in-One Microsen-Sor to the Laboratory Oven for Immediate Micro-Monitoring of the Roll-to-Roll Process of Polarizing Films
Authors: Lee Chi-Yuan , Liu Chen-Kai , Lin Jyun-Ting
Published: January 2023

The main purpose of this study is to carry out the immediate micro-monitoring of the roll-to-roll (R2R) process of polarizing films. Therefore, a self-made flexible three-in-one (temperature, humidity, and flow) microsensor is developed. The temperature and flow sensing area are 585 μm × 450 μm, the humidity sensing area is 1065 μm × 1035 μm, and the minimum line width is 15 μm. The micro-electro-mechanical systems (MEMS) technology was applied to integrate temperature, humidity, and flow sensors on a 50 µm thick polyimide substrate. A 100 Å thick chromium (Chrome, Cr) section form the adhesion layer. A 1000 Å thick gold section forms the sensing layer. A self-made flexible three-in-one microsensor set up in a laboratory oven for immediate micro-monitoring of the R2R process of the polarizing film. Since it is not advisable to set up signal lines in a clean room, the analog signals of the sensor should be transmitted via wireless means. Thus, a monitoring module should be connected to the back end of the self-made flexible three-in-one microsensor to receive the analog signals of the sensor, convert them into digital signals, send them out in the form of wireless signals, and store the data on the server-side. Through these measures, both the R2R process and yield can be improved. Therefore, the focus of this study is the environmental monitoring of drying process ovens. However, commercially available all-in-one sensors cannot handle the temperature of high-temperature factory ovens, and commercial flow sensors are rarely used in high-temperature applications. Some are also expensive and cannot be widely distributed, so this study intends to develop an integrated sensor to measure the internal environment of the drying oven.
Surface and Tribological Properties of Powder Metallurgical Cp-Ti Titanium Alloy Modified by Shot Peening
Authors: Avcu Yasemin Yıldıran , Iakovakis Eleftherios , Guney Mert , Çalım Emirhan , Özkılınç Ayşe , Abakay Eray , Sönmez Fikret , Koç Funda Gül , Yamanoğlu Rıdvan , Cengiz Abdulkadir , Avcu Egemen
Published: January 2023

The present study reveals for the first time the dry sliding wear behavior of a powder metallurgical pure titanium alloy (Cp-Ti) modified by shot peening. Cp-Ti samples were manufactured via powder metallurgy, and then their surface and subsurface features were modified using a custom-made, fully automated shot-peening system. The texture isotropy rate and the highest orientation angle of the shot-peened samples were 71.5% and 36°, respectively. The Abbott curves of the shot-peened surfaces revealed that the most common areal roughness value was 5.177 μm, with a frequency of 8.1%. Shot-peened surfaces exhibited an ~20% lower wear rate than unpeened surfaces under dry sliding wear, whereas the coefficient of friction was the same for both surfaces. Micro-ploughing, micro-cutting, oxidation, and three-body abrasion wear mechanisms were observed on the shot-peened and unpeened surfaces. High resolution 3D surface topographies of worn unpeened and shot-peened surfaces revealed micro-scratches and inhomogeneities along wear tracks, which are indicative of three-body abrasion mechanisms during contact. In addition, vertical and horizontal microcracks were visible just beneath the wear track, suggesting a clear indication of plastic deformation during contact. The cross-sectional hardness maps of shot-peened samples revealed the formation of a work-hardened surface layer with shot peening, which improved the wear resistance. These findings support that shot peening can be a useful tool to modify the surface and tribological properties of powder metallurgical Cp-Ti alloys.
CLCD-I: Cross-Language Clone Detection by Using Deep Learning with InferCode
Authors: Yahya Mohammad A. , Kim Dae-Kyoo
Published: January 2023

Source code clones are common in software development as part of reuse practice. However, they are also often a source of errors compromising software maintainability. The existing work on code clone detection mainly focuses on clones in a single programming language. However, nowadays software is increasingly developed on a multilanguage platform on which code is reused across different programming languages. Detecting code clones in such a platform is challenging and has not been studied much. In this paper, we present CLCD-I, a deep neural network-based approach for detecting cross-language code clones by using InferCode which is an embedding technique for source code. The design of our model is twofold: (a) taking as input InferCode embeddings of source code in two different programming languages and (b) forwarding them to a Siamese architecture for comparative processing. We compare the performance of CLCD-I with LSTM autoencoders and the existing approaches on cross-language code clone detection. The evaluation shows the CLCD-I outperforms LSTM autoencoders by 30% on average and the existing approaches by 15% on average.
The Molecular Characterization of Genetic Abnormalities in Esophageal Squamous Cell Carcinoma May Foster the Development of Targeted Therapies
Authors: Testa Ugo , Castelli Germana , Pelosi Elvira
Published: January 2023

Esophageal cancer is among the most common tumors in the world and is associated with poor outcomes, with a 5-year survival rate of about 10–20%. Two main histological subtypes are observed: esophageal squamous cell carcinoma (ESCC), more frequent among Asian populations, and esophageal adenocarcinoma (EAC), the predominant type in Western populations. The development of molecular analysis techniques has led to the definition of the molecular alterations observed in ESCC, consistently differing from those observed in EAC. The genetic alterations observed are complex and heterogeneous and involve gene mutations, gene deletions and gene amplifications. However, despite the consistent progress in the definition of the molecular basis of ESCC, precision oncology for these patients is still virtually absent. The recent identification of molecular subtypes of ESCC with clinical relevance may foster the development of new therapeutic strategies. It is estimated that about 40% of the genetic alterations observed in ESCC are actionable. Furthermore, the recent introduction of solid tumor immunotherapy with immune checkpoint inhibitors (ICIs) showed that a minority of ESCC patients are responsive, and the administration of ICIs, in combination with standard chemotherapy, significantly improves overall survival over chemotherapy in ESCC patients with advanced disease.
Quantifying the Impact of Family Doctors on the Care Experiences of Patients with Cancer: Exploring Evidence from the 2021 Ambulatory Oncology Patient Satisfaction Survey in Alberta, Canada
Authors: Watson Linda , Link Claire , Qi Siwei , DeIure Andrea
Published: January 2023

Oncology programs across Canada are reaching capacity as more Canadians are diagnosed with and treated for cancer each year. There is an increasing need to share care with family doctors, however it is unclear how this type of care impacts patient experiences, particularly while receiving active treatment. Retrospective data from the 2021 Ambulatory Oncology Patient Satisfaction Survey (AOPSS) in Alberta, Canada was used in this study. A unique question on the Alberta survey asks patients about their family doctor’s involvement during their cancer care. Patient satisfaction across the six domains of person-centred care on the AOPSS was analyzed based on how involved a patient’s family doctor was. Compared to patients who indicated their family doctor was “Not involved”, patients with “Very involved” family doctors had significantly higher satisfaction scores in all six domains of care. The three domains which showed the largest positive impact of family doctor involvement were: Coordination & Integration of Care, Emotional Concerns, and Information, Communication & Education. The results demonstrate that involving family doctors in cancer care can be beneficial for patients. Based on the observed satisfaction increases in this study, shared care models may be preferred by many patients. These models of care can also help alleviate strain and capacity issues within cancer programs. The results could be used to support recommendations for cancer care teams to regularly involve and communicate with family doctors, to ensure that patients receive comprehensive and tailored care from all their health care providers.
Progress on Geographical Distribution, Driving Factors and Ecological Functions of Nepalese Alder
Authors: Xia Chenxi , Zhao Wanglin , Wang Jinniu , Sun Jian , Cui Guangshuai , Zhang Lin
Published: January 2023

As the oldest species of Betulaceae, Nepalese alder (Alnus nepalensis) shows a high capacity for nitrogen fixation, rapid growth rate, and strong adaptability to stress environments, and it plays an important role in maintaining the structure and function of forest and agroforestry ecosystems. We explored its geographic distribution and the corresponding environmental drivers through collecting specimen records and published literature for Nepalese alder over the world during the past 40 years. The research trends, the growth limiting factors, the physiological characteristics, and ecological functions were all summarized as well. In terms of geographical distribution and limiting factors, Nepalese alder is mainly distributed in southern mountainous areas of the Himalayas and southwest China. Since it presented a clear northern limit of distribution and an upper limit of elevation, temperature is assumed to be the main environmental limiting factor. According to historical development, the research history of Nepalese alder could be divided into three main periods: the initial development (before 2001), the fast development (2002–2015), and the high-quality development (2016–2022), with the two key points in 2002 and 2015 relating to the conversion of cropland to a forest project that the government conducted and the application from theory to practice, respectively. As can be seen from the ecological functions, Nepalese alder could form symbiotic nodules with Frankia, which plays an important role in improving soil physical and chemical properties and facilitating vegetation secondary succession. Overall, the present review provides a reference for further studies on ecological adaptability and sustainable utilization of Nepalese alder under climate change, and also for regional ecosystem service, forestry production practice, and vegetation restoration.