bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental health care-seeking and barriers: a cross-sectional study of an urban Latinx community
Authors: Newberry Jennifer A. , Gimenez Michelle A. , Gunturkun Fatma , Villa Erica , Maldonado Maritza , Gonzalez Dilza , Garcia Gabriel , Espinosa Patricia Rodriguez , Hedlin Haley , Kaysen Debra
Published: November 2024

Background

The Latinx community faces an increasing amount of mental health challenges and disparities in care. While the contributing factors are complex, there are likely potential barriers related to connecting with mental health support and accessing care that can be addressed.

Methods

To investigate barriers in connecting to mental health care, we conducted a cross-sectional survey of mental health service use and barriers in an urban community with a primarily Hispanic/Latinx ethnicity using a modified random walk approach for door-to-door data collection with a two-cluster sampling frame. Survey included questions on socio-demographic, mental health status, desire and attempt to seek care, and the Barriers to Access to Care Evaluation. Shapley additive explanation (SHAP) identified impactful barriers and demographic characteristics. Our primary outcome was the number of respondents who saw a professional in the past 12 months and the key determinants that enabled their successful connection. Secondary outcomes were people with poor mental health who had wanted or tried to seek any source of mental health support.

Results

Of the 1004 respondents enrolled, 70.5% were foreign born; 63.4% were women. In the past 12 months, 23.8% of respondents wanted to connect with mental health care; 15.5% tried to connect, and only 11.7% successfully connected to mental health services. The two most cited barriers had the highest SHAP values: concerns about treatments available (65%) and financial costs (62.7%). Additional barriers with high SHAP values: being seen as weak and having no one to help them find care. Of demographic characteristics, age had the highest SHAP values.

Conclusion

In a community with a high density of Latinx immigrants, just under half of respondents wanting mental health care successfully connected. Perceived informational, financial, and stigma-related barriers impacted the likelihood to connect with mental health care. These factors should be considered when designing programs and interventions to improve mental health care access and services in the Latinx community.
Nature-based approaches: a mixed methods study of facilitators and barriers to implementation in CAMHS
Authors: Mitchell Siobhan B. , Chapman Beth , Hayes Rachel , Bonnici Hélène , Banks Hazel , Mareva Silvana , Hardwick Rebecca
Published: November 2024

Background

There is growing evidence that spending time with or in nature can be beneficial for health and wellbeing. Emerging evidence suggests potential benefits for staff and service users in healthcare settings, yet little is known about how to put Nature-based approaches (NBAs) into practice within the Child and Adolescent Mental Health Services (CAMHS) setting. The CAMHS Goes Wild project in Southwest England aimed to explore the implementation of NBAs within CAMHS, examining staff attitudes and understanding to identify potential benefits and challenges through a mixed methods study.

Methods

The study involved three phases of data collection: an online survey with two waves, the first wave prior to the training, and the second four months post-training, and semi-structured interviews. Data collection was designed to fit around NatureWell training, attended by sixty-four CAMHS staff, which took place alongside the study. All participants were sampled from one NHS Trust and the study was open to both those who had attended the NatureWell training and those who had not. Data were synthesised to produce an understanding of staff attitudes towards NBAs and perceived barriers and facilitators to the implementation of this approach.

Results

Ninety-seven staff responded to the wave 1 survey and 57 responded to the wave 2 survey. Fourteen staff members were interviewed. Data synthesis generated three themes: Tension between the culture of CAMHS and NBAs (Theme 1) and the need for buy-in and governance support (Theme 2). Theme three described the potential benefits of NBAs for staff and service users in CAMHS and is presented in a separate paper. The first two themes are presented in this paper.

Conclusions

The implementation of NBAs in mental health service settings for CYP presents both significant challenges and opportunities. Our findings suggest multiple barriers to implementation, often in the form of organisational or cultural factors, such as the risk averse nature of the service. Our work also elucidates several potential facilitators which may address or mitigate some of these barriers. These potential enablers, such as harnessing the role of firsthand experience, warrant further exploration in the implementation of NBAs in CAMHS.
Prognostic value of computed tomography-derived fractional flow reserve in patients with diabetes mellitus and unstable angina
Authors: Zhao Qi , Liu Li , Xian Huimin , Luo Xing , Zhang Donghui , Hou Shenglong , Qu Chao , Zhang Ruoxi , Qu Xiufen
Published: November 2024

Background

Coronary artery calcification is commonly found in patients with type 2 diabetes mellitus (T2DM), which may compromise the diagnostic accuracy of coronary computed tomography angiography (CTA). Computed tomography-derived fractional flow reserve (CT-FFR), which integrates coronary anatomy with functional assessment, holds the potential to become a powerful diagnostic tool for evaluating calcified lesions.

Objective

We aim to assess the prognostic value of CT-FFR for calcific lesions in patients with T2DM and unstable angina (UA).

Methods

We conducted a retrospective study involving 3,392 patients who were diagnosed with T2DM and UA who underwent coronary CTA, with at least one visible calcification site. Of those, 1,091 patients and 1,372 vessels were recommended by cardiovascular specialists and completed invasive coronary angiography (ICA) and invasive fractional flow reserve (FFR) measurements. Simultaneously, those patients also underwent CT-FFR measurements and were divided into two groups based on CT-FFR values: one group with CT-FFR > 0.80 and the other with CT-FFR ≤ 0.80. Demographics, clinical data, the diagnostic performance of CT-FFR, analysis of calcified lesions on CTA, and major adverse events during follow-up were recorded.

Results

The diagnostic accuracy, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the area under the curve (AUC) of CT-FFR were 84.8%, 84.6%, 85.1%, 84.7%, 85.0%, and 84.8%, respectively, per patient, and 82.2%, 80.3.2%, 81.8%, 79.7%, 81.1%, and 82.9% respectively, per vessel. For lesion and calcification characteristics, the degree of stenosis, lesion length, rate of bifurcation lesions, diffusive lesions, occlusion, calcium volume, and coronary artery calcification score (CACS) were significantly higher in the CT-FFR ≤ 0.8 group compared to the CT-FFR > 0.8 group. In contrast, the minimum cross-sectional area was smaller in the CT-FFR ≤ 0.8 group than in the CT-FFR > 0.8 group. Major adverse cardiovascular and cerebrovascular events (MACCE) at the 3-year follow-up was significantly higher in the CT-FFR ≤ 0.8 group compared to the CT-FFR > 0.8 group. The CT-FFR value is an independent predictor of MACCE at the 3-year follow-up.

Conclusion

CT-FFR demonstrated significant diagnostic performance using invasive FFR as the reference standard and proved to be an important predictive tool for assessing prognosis not only in calcified lesions but also in lesions with a CACS score of zero in patients with T2DM and UA. CT-FFR may serve as a valuable tool for guiding treatment decisions in these patients.

Graphical abstract
How to ensure an appropriate oral health workforce? Modelling future scenarios for the Netherlands
Authors: Janssen Jip , Pöld Ave , Islam Md Monirul , Németh Orsolya , Grytten Jostein , Woods Noel , Listl Stefan
Published: November 2024

Background

Current methods for oral health workforce planning lack responsiveness to dynamic needs, hampering efficiency, equity and sustainability. Effective workforce planning is vital for resilient health care systems and achieving universal health coverage. Given this context, we developed and operationalised a needs-adaptive oral health workforce planning model and explored the potential of various future scenarios.

Methods

Using publicly available data, including the Special Eurobarometer 330 Oral Health Survey, we applied the model in a hypothetical context focusing on the Dutch population’s dental needs from 2022 to 2050. We compared current and future provider supply and requirement and examined, in addition to a base case scenario, several alternative scenarios. These included epidemiological transition scenarios with different oral health morbidity trajectories, skill-mix scenarios with independent oral hygienists conducting check-ups and multiple dental student intake and training duration (5 instead of 6 years) scenarios.

Results

Based on the aforementioned historical data, our model projects that provider requirement will exceed supply for the planning period. If the percentage of people having all natural teeth increases by 10% or 20% in 2032, 34 or 68 additional full-time equivalent (FTE) dentists will be required, respectively, compared to the base case scenario. In the skill-mix scenario, the model indicates that prioritising oral hygienists for check-ups and shifting dentists’ focus to primarily complex care could address population needs more efficiently. Among the student intake and training duration scenarios, increasing intake to 375 and, to a lesser extent, reducing training to 5 years is projected to most effectively close the provider gap.

Conclusions

The study underscores the importance of understanding oral health morbidity trajectories for effective capacity planning. Due to limited dental epidemiological data, projections carry substantial uncertainty. Currently, demand for FTE dentists seems to exceed supply, though this may vary with epidemiological changes. Skill-mix strategies could offer efficiency gains by redistributing tasks, while adjustments in dental intake and training duration could also help address the requirement-supply gap. Resolving dentistry workforce challenges requires a multifaceted approach, including strengthening oral epidemiology projections, addressing the root causes of dental health issues and prioritising harmonious dental public health and general practice prevention measures.
Expediting treatments in the 21st century: orphan drugs and accelerated approvals
Authors: Domike Reuben , Raju G. K. , Sullivan Jamie , Kennedy Annie
Published: November 2024

Background

In response to activated patient communities’ catalyzation, two significant efforts by the FDA to expedite treatments have now been in place for multiple decades. In 1983, the United States Congress passed the Orphan Drug Act to provide financial incentives for development of drugs for rare diseases. In 1992, partly in response to the HIV epidemic, the FDA implemented Accelerated Approval (AA) to expedite access to promising new therapies to treat serious conditions with unmet medical need based on surrogate marker efficacy while additional clinical data is confirmed.

The uses of these regulatory approaches over time are assessed in this study.

Methods

The following U.S. FDA CDER published lists were used in this analysis: 1. all orphan designations and approvals; 2. all AA and their details updated through December 31, 2022; new molecular entities (NMEs).

Results

Orphan drug designations and approvals have increased several-fold over the past four decades. The largest increase recently has been in therapies targeting oncological diseases (comprised of both oncology and malignant hematology).

Although orphan drug approvals based on NMEs are the minority of orphan drug designations, the count of approved orphan drug NMEs has increased in recent years.

The characteristics of orphan drug approvals show notable differences by disease area with rare diseases and medical genetics (49%) having a relatively large fraction of orphan drug approvals with NMEs compared to the oncological diseases (32%).

Similar to the use of orphan drug designation, oncological disease therapies have been the largest utilizers of AA. Many therapies targeting these diseases address unmet medical need and can leverage surrogate markers that have previously been used in similar trials.

The timings of conversion of AA (confirmed or withdrawn) were assessed and found to be consistent across decades and to have some dependency upon the broad disease area (when assessed by three large groups: HIV conversions were fastest; followed by oncology; followed by all others). By the end of 2022, 98% of the first 105 (approved in 2010 or earlier) AA had been converted to confirmed or withdrawn.

Conclusions

Although the typical timings for AA to be confirmed or withdrawn has not changed significantly over the decades, the disease areas utilizing orphan drug designation and AA have changed significantly over time. Both programs have had increases in their use for therapies targeting oncological diseases.

The re-use of surrogate markers for oncological diseases has been an advantage in a way that may not be scientifically feasible in many other disease areas that have greater differentiation across disease etiology. For non-oncological diseases, applicability of AA is, in part, dependent upon greater focus on characterization and acceptance of novel surrogate markers.
Validation of a guidelines-based digital tool to assess the need for germline cancer genetic testing
Authors: Russell Callan D. , Daley Ashley V. , Van Arnem Durand R. , Hila Andi V. , Johnson Kiley J. , Davies Jill N. , Cytron Hanah S. , Ready Kaylene J. , Armstrong Cary M. , Sylvester Mark E. , Caleshu Colleen A.
Published: November 2024

Background

Efficient and scalable solutions are needed to identify patients who qualify for germline cancer genetic testing. We evaluated the clinical validity of a brief, patient-administered hereditary cancer risk assessment digital tool programmed to assess if patients meet criteria for germline genetic testing, based on personal and family history, and in line with national guidelines.

Methods

We applied the tool to cases seen in a nationwide telehealth genetic counseling practice. Validity of the tool was evaluated by comparing the tool’s assessment to that of the genetic counselor who saw the patient. Patients’ histories were extracted from genetic counselor-collected pedigrees and input into the tool by the research team to model how a patient would complete the tool. We also validated the tool’s assessment of which specific aspects of the personal and family history met criteria for genetic testing. Descriptive statistics were used.

Results

Of the 152 cases (80% female, mean age 52.3), 56% had a personal history of cancer and 66% met genetic testing criteria. The tool and genetic counselor agreed in 96% of cases. Most disagreements (4/6; 67%) occurred because the genetic counselor’s assessment relied on details the tool was not programmed to collect since patients typically don’t have access to the relevant information (pathology details, risk models). We also found complete agreement between the tool and research team on which specific aspects of the patient’s history met criteria for genetic testing.

Conclusion

We observed a high level of agreement with genetic counselor assessments, affirming the tool’s clinical validity in identifying individuals for hereditary cancer predisposition testing and its potential for increasing access to hereditary cancer risk assessment.
Proceedings from an international consensus meeting on ablation in urogenital diseases
Authors: Iezzi Roberto , Contegiacomo Andrea , De Filippis Alessandra , Gunn Andrew J. , Atwell Thomas , Mcclure Timothy , Jing Zhang , Posa Alessandro , Scrofani Anna Rita , Maresca Alessandro , Madoff David C. , Goldberg Shraga Nahum , Kelekis Alexis , Filippiadis Dimitri , Sala Evis , Ahmed Muneeb
Published: November 2024

Percutaneous image-guided ablation techniques are a consolidated therapeutic alternative for patients with high preoperative surgical risk for the management of oncological diseases in multiple body districts. Each technique has both pros and cons according to the type of energy delivered, mechanism of action, and site of application. The present article reviews the most recent literature results on ablation techniques applied in the field of genitourinary diseases (kidney, adrenal glands, prostate, and uterus), describing the advantages of the use of each technique and their technical limitations and summarizing the major recommendations from an international consensus meeting.

Critical relevant statement

The article critically evaluates the efficacy and safety of ablation therapies for various genitourinary tract diseases, demonstrating their potential to improve patient outcomes and advance clinical radiology by offering minimally invasive, effective alternatives to traditional surgical treatments.

Key Points

Ablation therapies are effective alternatives to surgery for renal cell carcinoma.

Ablation techniques offer effective treatment for intermediate-risk prostate cancer.

Ablation is a promising tool for adrenal tumor management.

Ablation reduces fibroid symptoms and volume, offering an alternative to surgery.

Graphical Abstract
Design of two-dimensional reflective imaging systems: an approach based on inverse methods
Authors: Verma Sanjana , Anthonissen Martijn J. H. , ten Thije Boonkkamp Jan H. M. , IJzerman Wilbert L.
Published: November 2024

Imaging systems are inherently prone to aberrations. We present an optimization method to design two-dimensional (2D) freeform reflectors that minimize aberrations for various parallel ray beams incident on the optical system. We iteratively design reflectors using inverse methods from non-imaging optics and optimize them to obtain a system that produces minimal aberrations. This is done by minimizing a merit function that quantifies aberrations and is dependent on the energy distributions at the source and target of an optical system, which are input parameters essential for inverse freeform design. The proposed method is tested for two configurations: a single-reflector system and a double-reflector system. Classical designs consisting of aspheric elements are well-known for their ability to minimize aberrations. We compare the performance of our freeform optical elements with classical designs. The optimized freeform designs outperform the classical designs in both configurations.
Metabolomic profiling and 16 S rRNA metabarcoding of endophytes of two Aloe species revealed diverse metabolites
Authors: Marokane-Radebe Cynthia , Raimi Adekunle , Amoo Stephen , Adeleke Rasheed
Published: November 2024

Aloe species are often used interchangeably for medicinal and cosmeceutical applications, presenting a challenge to the biological efficacy consistency of some herbal preparations. Sustainable production of high-quality commonly used medicinal plants remains a limitation for commercialisation. Thus, this study investigated the potential for plant substitution by examining bacterial endophytes capable of producing similar host plant secondary metabolites. The metabolite profiles and endophytic bacterial communities of endangered Aloe lettyae were compared with those of Aloe longibracteata using nuclear magnetic resonance spectroscopy and 16 S rRNA gene sequencing. Only 15 metabolites were significantly different between A. lettyae and A. longibracteata based on metabolite concentrations. However, both plants’ functionality and potential application remain comparable. Phytohormones, including indole-3-acetate and 5-hydroxyindole-3-acetate, were more concentrated in A. lettyae than A. longibracteata. Metabolites such as tyrosine, allantoin, and myo-inositol, with human health benefits, were annotated in both species. Aloe lettyae harboured a phylogenetically diverse bacteria community compared to A. longibracteata, with a higher richness of bacterial species, indicating a likelihood of diverse metabolic capabilities among the bacteria. Dominant endophytes, including Bacillus, Comamonas, and Pseudomonas, possess enzymes contributing to various metabolic pathways. The enzymes have the potential to impact the synthesis, or breakdown of plant metabolites, consequently influencing the overall metabolic composition of the host plant. Therefore, this study supports the interchangeability of A. lettyae and A. longibracteata due to their ability to produce similar metabolites, and although the Aloe species exhibit phylogenetically diverse endophytic communities, the feasibility of utilizing their endophytes as producers of secondary metabolites remains viable.

Graphical abstract
Environmental cleaning barriers and mitigation measures identified through two initiatives in four countries, 2018–2023: a commentary
Authors: Patrick Molly , Kilpatrick Claire , Storr Julie , Gon Giorgia , Huynh Tuan , Thang Phung Manh , Adeniyi Damilola , Ogunsola Folasade , Manzi Fatuma , Por Ir , Sarpong Bernice , Sedekia Yovitha , Sokvy Ma , Tang Vouchnea , Vong Sreytouch , Graham Wendy
Published: November 2024

In recent years, there has been increased attention on the importance of healthcare environmental cleaning, including the need to professionalize and support the workforce responsible for performing cleaning. Global agendas and strategies on infection prevention and control (IPC) and water, sanitation and hygiene highlight the need for improvements to this sector, particularly in resource-limited healthcare facilities in low- and middle-income countries. Correspondingly, several resources have been developed that aim to (1) improve professional training of cleaners and (2) improve implementation of best practices in resource-limited settings. This commentary seeks to provide insight into the barriers and facilitators to implementing these resources, drawing on the practical experience from two initiatives across four countries from 2018 through 2023. Several common barriers were identified across the diverse settings, including (1) low empowerment and status of the workforce, (2) low pay, inadequate staff time for the high workload needed to achieve best practices and high turnover of staff, and (3) a lack of connection and integration of environmental cleaning with IPC and patient safety efforts at the participating hospitals. Despite barriers, local teams identified effective mitigation measures. While considerable time and effort will be needed to truly overcome these barriers, there are opportunities to build upon attention and momentum on this topic and IPC initiatives in resource-limited settings in low- and middle-income countries. We propose several broader actions, all of which require local leadership and context-specific approaches.
The gut microbial metabolite indole-3-aldehyde alleviates impaired intestinal development by promoting intestinal stem cell expansion in weaned piglets
Authors: Zhang Jiaqi , Chen Yahui , Guo Xin , Li Xuan , Zhang Ruofan , Wang Mengting , Zhu Weiyun , Yu Kaifan
Published: November 2024

Background

Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets.

Results

In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids.

Conclusions

These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.
Plant-derived saponins and their prospective for cosmetic and personal care products
Authors: Kanlayavattanakul Mayuree , Mersni Donia , Lourith Nattaya
Published: November 2024

Plants are industrially cultivated and processed serving for specified sectors for human consumptions including cosmetic and personal care products. Where, the consumers’ awareness towards sustainability are increasing year by year. Among which, those of the materials derived from the plants produced with good agricultural and manufacturing practices abided with bio-circular-green economy theme, are of eminence. This perspective is in line with the researchers’ bioprospective onto natural products. Special attention sheds on saponins, the biosurfactants that will not cause detrimental effects on the environment. Which, plants are regarded as the sustainable sources of these cosmetic substances. However, among tremendous plants that have been continuously explored upon their potential applications. Most of the studies focus on preparation of the saponins and biological activities. Surprisingly, those that are abided with the list published in the European Commission (CosIng) that are of crucially for cosmetic regulation are insufficiently demonstrated, which burden their applications in the sector. This context summarizes the industrial crops that are registered as plant saponin in the CosIng database. Those that are insufficiently exploited on the information required for cosmetic formulations are therefore encouraged to be examined. In addition, multidirectional cosmetic beneficials of the filled plants saponin would be encouraged to be explored. These plants will be properly knowledge managed for their sustainable utilizations as the bio-based materials promising for cosmetic and personal care industrial perspectives.
Employing automatic analysis tools aligned to learning progressions to assess knowledge application and support learning in STEM
Authors: Kaldaras Leonora , Haudek Kevin , Krajcik Joseph
Published: November 2024

We discuss transforming STEM education using three aspects: learning progressions (LPs), constructed response performance assessments, and artificial intelligence (AI). Using LPs to inform instruction, curriculum, and assessment design helps foster students’ ability to apply content and practices to explain phenomena, which reflects deeper science understanding. To measure the progress along these LPs, performance assessments combining elements of disciplinary ideas, crosscutting concepts and practices are needed. However, these tasks are time-consuming and expensive to score and provide feedback for. Artificial intelligence (AI) allows to validate the LPs and evaluate performance assessments for many students quickly and efficiently. The evaluation provides a report describing student progress along LP and the supports needed to attain a higher LP level. We suggest using unsupervised, semi-supervised ML and generative AI (GAI) at early LP validation stages to identify relevant proficiency patterns and start building an LP. We further suggest employing supervised ML and GAI for developing targeted LP-aligned performance assessment for more accurate performance diagnosis at advanced LP validation stages. Finally, we discuss employing AI for designing automatic feedback systems for providing personalized feedback to students and helping teachers implement LP-based learning. We discuss the challenges of realizing these tasks and propose future research avenues.
Material decomposition approaches for monosodium urate (MSU) quantification in gouty arthritis: a (bio)phantom study
Authors: Diekhoff Torsten , Schmolke Sydney Alexandra , Khayata Karim , Mews Jürgen , Kotlyarov Maximilian
Published: November 2024

Background

Dual-energy computed tomography (DECT) is a noninvasive diagnostic tool for gouty arthritis. This study aimed to compare two postprocessing techniques for monosodium urate (MSU) detection: conventional two-material decomposition and material map-based decomposition.

Methods

A raster phantom and an ex vivo biophantom, embedded with four different MSU concentrations, were scanned in two high-end CT scanners. Scanner 1 used the conventional postprocessing method while scanner 2 employed the material map approach. Volumetric analysis was performed to determine MSU detection, and image quality parameters, such as signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR), were computed.

Results

The material map-based method demonstrated superior MSU detection. Specifically, scanner 2 yielded total MSU volumes of 5.29 ± 0.28 mL and 4.52 ± 0.29 mL (mean ± standard deviation) in the raster and biophantom, respectively, versus 2.35 ± 0.23 mL and 1.15 ± 0.17 mL for scanner 1. Radiation dose correlated positively with detection for the conventional scanner, while there was no such correlation for the material map-based decomposition method in the biophantom. Despite its higher detection rate, material map-based decomposition was inferior in terms of SNR, CNR, and artifacts.

Conclusion

While material map-based decomposition resulted in superior MSU detection, it is limited by challenges such as increased artifacts. Our findings highlight the potential of this method for gout diagnosis while underscoring the need for further research to enhance its clinical reliability.

Relevance statement

Advanced postprocessing such as material-map-based two-material decomposition might improve the sensitivity for gouty arthritis in clinical practice, thus, allowing for lower radiation doses or better sensitivity for gouty tophi.

Key Points

Dual-energy CT showed limited sensitivity for tophi with low MSU concentrations.

Materiel-map-based decomposition increased sensitivity compared to conventional two-material decomposition.

The advantages of material-map-based decomposition outweigh lower image quality and increased artifact load.

Graphical Abstract
Examining the standalone efficacy and safety of tranexamic acid in chronic subdural hematoma: a comprehensive review and meta-analysis
Authors: Ali Syed Muhammad Sinaan , Haseeb Abdul , Shafique Muhammad Ashir , Mustafa Muhammad Saqlain , Kumar Aashish , Nasir Rabia , Azhar Muhammad Abdullah Bin , Ahmad Tagwa Kalool Fadlalla , Raja Adarsh , Raja Sandesh , Lucke-Wold Brandon
Published: November 2024

Background

Chronic subdural hematoma (CSDH) is an important medical condition characterized by the accumulation of blood in the subdural space. Several methods have been explored, including surgical intervention and administration of tranexamic acid, which is a conservative treatment option. This meta-analysis aimed to assess the effectiveness and safety of tranexamic acid in CSDH management.

Methods

A systematic literature search was performed according to the PRISMA and MOOSE guidelines. This study included cohort and randomized controlled trials involving adults with chronic subdural hematoma (CSDH) who were treated with intravenous tranexamic acid. The primary outcome measures were recurrence and adverse events.

Results

Six studies encompassing 2024 participants were included. Studies have shown that tranexamic acid reduced recurrence rates (OR, 0.32; 95% CI: 0.27–0.38, p = 0.16; I2 = 0%), although the result was non-significant. However, the analysis of adverse events indicated a moderate level of heterogeneity (OR, 1.14; 95% CI: 0.60–2.15, p = 0.07; I2 = 57%), suggesting a potential safety concern.

Conclusion

In conclusion, this meta-analysis suggests tranexamic acid (TXA) may reduce chronic subdural hematoma (CSDH) recurrence, though significance varied across studies. Adverse effects with TXA were similar to controls. Larger trials are needed to confirm TXA’s role in CSDH management.
Hatchery type influences the gill microbiome of Atlantic farmed salmon (Salmo salar) after transfer to sea
Authors: Stewart Kelly J. , Boerlage Annette S. , Barr William , Ijaz Umer Z. , Smith Cindy J.
Published: November 2024

Background

Salmon aquaculture involves freshwater and seawater phases. Recently there has been an increase in multifactorial gill health challenges during the seawater phase which has led to an urgent need to understand the gill microbiome. There is a lack of understanding on what drives the composition of the gill microbiome, and the influence the freshwater stage has on its long-term composition. We characterise the gill microbiome from seven cohorts of Atlantic salmon raised in six different freshwater operational systems—recirculating aquaculture system (RAS), flowthrough (FT) and loch-based system, prior to and after transfer to seven seawater farms, over two different input seasons, S0 (2018) and S1 (2019).

Results

Using the V1-V2 region of the 16S rRNA gene, we produced amplicon libraries absent of host contamination. We showed that hatchery system influenced the gill microbiome (PERMAOVA R2 = 0.226, p < 0.001). Loch and FT systems were more similar to each other than the three RAS systems, which clustered together. On transfer to sea, the gill microbiomes of all fish changed and became more similar irrespective of the initial hatchery system, seawater farm location or season of input. Even though the gill microbiome among seawater farm locations were different between locations (PERMAOVA R2 = 0.528, p < 0.001), a clustering of the gill microbiomes by hatchery system of origin was still observed 7–25 days after transfer (PERMAOVA R = 0.164, p < 0.001). Core microbiomes at genera level were observed among all fish in addition to freshwater only, and seawater only. At ASV level core microbiomes were observed among FT and loch freshwater systems only and among all seawater salmon. The gill microbiome and surrounding water at each hatchery had more shared ASVs than seawater farms.

Conclusion

We showed hatchery system, loch, FT or RAS, significantly impacted the gill microbiome. On transfer to sea, the microbiomes changed and became more similar. After transfer, the individual sites to which the fish were transferred has a significant influence on microbiome composition, but interesting some clustering by hatchery system remained. Future gill disease mitigation methods that target enhancing the gill microbiome may be most effective in the freshwater stage, as there were more shared ASVs between water and gill at hatchery, compared to at sea.
Antioxidant activity, enzyme inhibition, photoprotection, cytotoxicity, and phytochemical profiling of sea lavender (Limonium algarvense Erben) seed extracts for dermo-cosmetic use
Authors: Lescano Leonardo , Cziáky Zoltán , Kurt-Celep İnci , Zengin Gökhan , Fernandes Eliana , Trentin Riccardo , Pereira Catarina G. , Custódio Luísa , Rodrigues Maria João
Published: November 2024

Background

Despite sea lavender being a medicinal species, research on its seeds’ biological properties and chemical composition is unexplored. Thus, this study evaluated the effect of different extraction solvents on the biological activities and chemical profile of greenhouse-cultivated sea lavender seeds, aiming at their potential use as a dermo-cosmetic ingredient. Therefore, ethyl acetate, acetone, ethanol, and water extracts were examined for their antioxidant activity, enzyme inhibition, photoprotection, and cytotoxicity, followed by phytochemical analysis through spectrophotometric methods, further detailed by Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Mass/Mass Spectrometry (UHPLC-Esi-MS/MS).

Results

The water extract demonstrated significant antioxidant activity, evidenced by low half maximal effective concentration (EC50) values in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing iron and chelating copper (296, 478, 230 and 678 µg/mL, respectively). The ethanol extract was more effective in inhibiting cosmetic-related enzymes, particularly elastase and hyaluronidase (2.18 and 3.21 µg/mL, respectively). The water and acetone extracts had the highest sun protection factors (23.2 and 18.9, respectively). All the extracts had nil to weak cytotoxicity (70–120% cell viability) towards mammalian cell lines. The water extract had the highest phenolics and condensed tannins (115 and 78.30 mg/g extract, respectively), while the ethanol contained the most flavonoids (62.73 mg/g extract). UHPLC-ESI–MS/MS analysis identified ethyl gallate, myricetin, rutin, and quercetin as major components of the ethanol extract, whereas myricetin-O-rutinoside isomers are predominant in the water extract.

Conclusions

These findings highlight the potential of greenhouse-cultivated sea lavender seeds as potential dermo-cosmetic ingredients, with ethanol and water extracts demonstrating superior biological activities and chemical profiles, significantly contributing to general skin health and protection.
Drug repurposing in the treatment of chronic inflammatory diseases
Authors: Sarup Shivmuni , Obukhov Alexander G. , Raizada Shubhi , Atre Rajat , Baig Mirza S.
Published: November 2024

Background

Chronic inflammation is an increasing global healthcare challenge with limited effective treatment options. Developing medications for chronic diseases requires high financial investment and a long duration. Given these challenges, alternative strategies are needed. Here, we focus on one such strategy that holds great promise: drug repurposing, which involves identifying new therapeutic uses for existing drugs.

Main body

Here, we discuss the importance of two key transcription factors: nuclear factor kappa B (NF-κB) and activator protein 1 (AP-1), in orchestrating complex pathophysiological signaling networks involved in chronic inflammatory diseases. Dysregulation of the NF-κB and AP1 signaling pathways have been associated with various diseases, such as cancer, inflammatory disease, and autoimmune disorders. This review emphasized that repurposed small-molecule inhibitors of these pathways have proven successful as therapeutic interventions. These compounds exhibit high degrees of specificity and efficacy in modulating NF-κB or AP-1 signaling, making them appealing candidates for treating chronic inflammatory conditions. This review discusses the therapeutic potential and action mechanisms of several repurposed small-molecule inhibitors for combating diseases caused by abnormal activation or inhibition of NF-κB and AP-1.

Conclusion

This concise review highlights the potential of repurposing small-molecule inhibitors targeting the NF-κB and AP-1 pathways as effective therapies for various chronic inflammatory diseases. While further experimental validation is needed, drug repurposing offers a promising strategy to bypass the existing lengthy and expensive new drug development processes, providing a faster and more economical route to novel treatments.
Development and evaluatıon of the Turkısh mobıle real-noıse speech dıscrımınatıon test
Authors: Ozkan Melek Basak , Konukseven Ozlem
Published: November 2024

Background

While there is a list of monosyllabic words available for use in speaking tests in our country, the list of sentences is limited. The aim of this study is to evaluate the effectiveness of the mobile application we developed by creating a list of 4-word sentences that are frequently encountered in daily life, well-known, and spectrally balanced, to be used in speech tests in children aged 8–12 with normal hearing. A total of 50 participants, 25 girls (mean age 9.56 ± 1.56) and 25 boys (mean age 10.60 ± 1.55) with normal hearing, aged 8–12, were included in the study. Familiarity was evaluated by listening to a list of 200 sentences consisting of 4-word sentences to 70 children in 7 geographical regions of Turkey, and the sentences that 80% of the children did not understand were excluded. A list of 60 balanced sentences was determined by performing spectral and coherence analysis of the sentences. The recording of each sentence at 80 dB, 60 dB, and 40 dB intensity levels, respectively, increased in 5 dB steps between the − 10/ + 10 signal-to-noise ratio value and was combined separately with 3 different environmental noises (classroom, shopping mall, amusement park) and uploaded to the developed mobile application.

Results

For all participants, speech comprehension scores at 80 dB, 60 dB, and 40 dB sound intensity were lowest in the amusement park environment. Speech comprehension scores are lowest in noise levels of 40 dB in amusement park and shopping mall environments. Speech comprehension scores are lowest in noise levels of 60 dB in the classroom environment. There is a statistically significant difference between ambient noises (p < 0.05). When male and female participants are compared, it is seen that male participants’ comprehension scores in noise in the shopping mall environment are higher than female participants. There is a statistically significant difference between genders (p < 0.05).

Conclusion

When the speech in noise tests developed in the literature are examined, even though the word lists are equalized with the phonemic equalization method, they do not reflect the reality of daily life. The Turkish Mobile Speech Discrimination in Real Noise 4-Word Sentence Test that we have developed will contribute to the literature. It is recommended that future research compare the results by adding different ambient noises.
Influence of particle density on turbulence characteristics over a rough surface
Authors: Singh Kirti , Prasad Kesheo
Published: November 2024

The objective of the study is to use a 3D Acoustic Doppler Velocimeter (ADV) to gauge the typical flow and turbulence characteristics within a non-uniform open channel. The findings of experimental examinations of the subcritical flow along the channel are presented in this work. The behavior of sand grains in turbulent open channel flow across porous and rough bed surfaces was examined in laboratory research, and the results were obtained. The properties of turbulent flow, i.e., turbulence intensity, turbulent kinetic energy, and Reynolds shear stresses, are determined from ADV data. The continuity equation and the Reynolds equation of open-channel flow have been used to build theoretical formulations for the velocity distribution and Reynolds stress distribution in the vertical direction. Measured profiles of vertical velocity and Reynolds stress are compared to the derived expressions. The impact of the size of particles on the distribution of mean flow characteristics is discussed. This work provides a novel origin for the profile and analyzes the behavior of the vertical velocity distribution in the region where fully formed turbulence is dominating in open channels using the Navier–Stokes equations. In comparison to other sand roughness, Chopan sand bed (with greater density) exhibits the strongest turbulence intensities in both vertical and streamwise direction just next to the bed when away from the channel boundary. In contrast to flow across a rough surface, the variance ranges between 150 and 250% concerning the channel bed’s roughness type, impacting the velocity triple products that signifies transfer of turbulent kinetic energy.
Pharmacodynamic evaluation and network pharmacology analysis of a novel anti-heat stress Chinese herbal formula
Authors: Wang Hanfei , Xu Shuyi , Mao Haiyang , Wang Boyu , Feng Yanping , Ihsan Awais , Li Shijun , Wang Xu
Published: November 2024

Frequent extreme heat events around the world not only pose a major threat to human health but also cause significant economic losses to the livestock industry. The existing management practices are insufficient to fully prevent heat stress with an urgent need to develop preventive medicines. The aim of this study was to develop an anti-heat stress Chinese herbal formula (CHF) via big data analysis techniques and to evaluate its anti-heat stress effect and mechanism of action via pharmacodynamic evaluation and network pharmacology analysis. Many anti-heat stress CHFs were collected from the Chinese National Knowledge Infrastructure (CNKI) database. Three alternative CHFs were obtained via unsupervised entropy hierarchical clustering analysis, and the most effective CHF against heat stress, Shidi Jieshu decoction (SJD), was obtained by screening in a mouse heat stress model. In dry and hot environments, SJD significantly improved the heat tolerance of AA broilers by 4–6°C. In a humid and hot environment, pretreatment with 2% SJD resulted in 100% survival of Wenchang chickens at high temperatures. The main active ingredients of SJD were identified as muntjacoside E, timosaponin C, macrostemonoside H and mangiferin via ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) and database comparison. The active ingredients of SJD were found to target tumor necrosis factor-α (TNF-α), signal transducer activator of transcription 3 (STAT3) and epidermal growth factor receptor (EGFR). Finally, the safety of the new formulation was assessed in an acute oral toxicity study in rats. The SJDs developed in this study provide a new option for the prevention of heat stress in animal husbandry and offer new insights for further research on anti-heat stress.