bluuebunny/crossref_metadata_2025 huggingface dataset:
Pre-, Per- and post-cooling strategies used by competitive tennis players in hot dry and hot humid conditions
Authors: Robin Nicolas , Crespo Miguel , Ishihara Toru , Carien Robbin , Brechbuhl Cyril , Hue Olivier , Dominique Laurent
Published: September 2024

Purpose

This research investigated the pre-, per- and post cooling strategies used by competitive tennis players from various levels of play who occasionally train and compete in hot (>28°C) and humid (>60% rH), and dry (<60% rH) environments.

Methods

129 male tennis players (Mage = 24.9) competing at regional (N = 54), national (N = 30) and international (N = 45) levels, completed an online questionnaire regarding their use (i.e., timing, type, justification and effectiveness) of pre- (i.e., before practice), per- (i.e., during exercise) and post-cooling strategies when playing tennis in hot dry (HD) and hot humid (HH) conditions. Individual follow-up interviews were also carried on 3 participants to gain an in-depth understanding of the player's experience.

Results

Competitive tennis players used both internal and external cooling strategies to combat the negative effects of HD and HH conditions, but considered the HH to be more stressful than HD and experienced more heat-related illness in HH environments. International players used cold packs and cold towel more frequently than the regional and national players in hot environments, and used cold water immersion and cold vest more frequently than the latter in HH. Differences in strategy use were mostly observed during per-cooling where regional and national players more frequently used cold drinks than international players who more frequently used cold packs in HD and cold towel in HH conditions. Moreover the latter more frequently used cold towel, cold packs and cold water immersion as post-cooling strategies than regional players.

Conclusion

When playing tennis in the heat, it is strongly recommended to employ cooling strategies to maintain health, limit declines in performance, and promote recovery. We also recommend improving education regarding the appropriate use and effectiveness of cooling strategies, and increasing their availability in tournaments.
Enhancement of the energy and exergy analysis capabilities of the yoghurt process: a case study of the dairy industry
Authors: Oztuna Taner Oznur
Published: September 2024

This study provides a comprehensive analysis of the thermal and exergy characteristics of a dairy plant that produces yoghurt. This study aims to perform a comprehensive analysis of the thermal and exergy aspects of a dairy facility that produces yoghurt. This study also seeks to improve the accuracy of the results by evaluating the reliability of the energy and production data. A comprehensive analysis of energy and exergy is utilized to enhance the yoghurt production process. Moreover, the Grassmann-Sankey diagram is employed to produce a map of energy density. The process’s energy and exergy efficiencies were assessed by taking into account the enhancements and alterations made in addition to the existing implementations. Analysis of the yoghurt production process revealed that the total energy input was 113.9 [kW], the total energy output was 72.05 kW as well and the energy efficiency was 63.3%. The exergy input and output for the yoghurt production process were calculated to be 48.95 [kW] and 29.77 [kW], and the exergy efficiency was determined to be 60.8%. This study is expected to promote the growth of livestock and agriculture in the energy sector, and is forecasted to act as a catalyst for future research. This study, which is the first of its kind in the region and is expected to stimulate further research, reveals that improving energy efficiency and conservation in the production of yoghurt products enhances the factory’s overall energy efficiency.
Multi-Objective Optimization of the Pre-Swirl System in a Twin-Web Turbine Disc Cavity
Authors: Guo Yueteng , Wang Suofang , Shen Wenjie
Published: September 2024

Enhancing thermal efficiency and minimizing weight are prevailing issues in aero engines. Owing to its hollow structure, the twin-web turbine disc exhibits remarkable weight reduction properties, while its enhanced cooling constitutes a novel challenge. In this study, a twin-web turbine disc cavity system is numerically investigated. To enhance the cooling effect and minimize pressure loss, a multi-objective genetic algorithm and Kriging surrogate model are employed to optimize the radial height of the pre-swirl nozzle and receiver hole in the disc cavity system. The results indicate that the overall performance of Opt-3, derived from the Technique for Order Preference by Similarity to the Ideal Solution method within the Pareto frontier, is superior. This configuration achieves a uniform low distribution of rotor temperatures while maintaining moderate pressure losses. Notably, the maximum temperature is reduced by 21.1 K compared to the basic model, with pressure losses remaining largely unchanged. Additionally, an increase in the flow ratio leads to a reduction in both the maximum temperature and average temperature of the back web while simultaneously increasing the temperature of the front web and augmenting pressure losses. However, it is important to note that the degree of variation in these parameters diminishes with increasing flow ratios.
Consensus SE(3)-Constrained Extended Kalman Filter for Close Proximity Orbital Relative Pose Estimation
Authors: Mathavaraj S. , Butcher Eric A.
Published: September 2024

In this paper, a recently proposed SE(3)-constrained extended Kalman filter (EKF) is extended to formulate a strategy for relative orbit estimation in a space-based sensor network. The resulting consensus SE(3)-constrained EKF utilizes space-based sensor fusion and is applied to the problem of spacecraft proximity operations and formation flying. The proposed filter allows for the state (i.e., pose and velocities) estimation of the deputy satellite while accounting for measurement error statistics using the rotation matrix to represent attitude. Via a comparison with a conventional filter in the literature, it is shown that the use of the proposed consensus SE(3)-constrained EKF can improve the convergence performance of the existing filter for satellite formation flying. Moreover, the benefits of faster convergence and consensus speed by using communication networks with more connections are illustrated to show the significance of the proposed sensor fusion strategy in spacecraft proximity operations.
Virtual Dynamic Vibration Absorber Trap Fusion Active Vibration Suppression Algorithm Based on Inertial Actuators for Large Flexible Space Trusses
Authors: Qin Chao , Xu Anpeng , He Shuai , Han Chunyang , Xu Zhenbang
Published: September 2024

This paper presents a virtual dynamic vibration absorber (DVA) trap fusion active vibration suppression algorithm based on inertial actuators as a solution to the harmonic vibration control problem of large flexible space trusses. Firstly, the mechanism of the inertial actuator is analyzed, and the relationship between the bandwidth of the algorithm and the intrinsic frequency of the inertial actuator is derived. Secondly, a dynamic model of the space truss is constructed. Subsequently, an analysis is conducted to determine the manner in which the virtual DVA exerts influence on the system’s dynamic characteristics. Based on this analysis, a virtual DVA trap fusion active vibration suppression algorithm is designed. Finally, the efficacy of the proposed algorithm in suppressing vibration is demonstrated through experimentation. The algorithm was demonstrated to be effective in suppressing both single-frequency harmonic vibration and multi-frequency harmonic vibration under the working conditions of single-degree-of-freedom and multi-degree-of-freedom of a flexible truss. The vibration suppression efficiency was found to be greater than 60%. It is therefore evident that the proposed algorithm has the potential to be applied to the vibration suppression of telescopes assembled in orbit in the future.
Investigation of the Traveling Performance of the Tracked Chassis of a Potato Combine Harvester in Hilly and Mountainous Areas
Authors: Chen Yangzhou , Wang Zeyu , Zhang Hua , Liu Xiaolong , Li Hui , Sun Wei , Li Hongling
Published: September 2024

Aiming at the problems of poor passability of a tracked chassis due to small plots, complicated road conditions and steep slopes during mechanized potato harvesting in hilly and mountainous areas. To design a potato combine harvester and take the tracked chassis of the harvester as the research object to study its driving performance under typical road conditions in mountainous areas. Firstly, mechanical analysis and theoretical calculation are carried out on the tracked chassis to obtain the relevant parameters of key components, and secondly, its driving performance is analyzed to obtain the driving limit values of passing performance under different working conditions; RecurDyn software was used to establish the dynamics simulation and analysis model of the whole machine, and the driving limit values of the harvester were determined under five different road conditions through simulation. The results show the following: the driving limit gradient angle is 20° in cross-gradient conditions, 25° in longitudinal up-gradient conditions, 25° in longitudinal down-gradient conditions, the limiting height of the chassis that can be overpassed in obstacle-crossing conditions is 450 mm, and the limiting width of the chassis that can be spanned in trench-crossing conditions is 1200 mm. The simulation results were verified through field tests, and the results of the field tests showed that the harvester met the requirements of stable travelling on longitudinal slopes of 24°, climbed over a 450 mm straight wall and crossed over a 1200 mm trench, which were similar to the simulation results, indicating that the simulation results were accurate and feasible, and met the design requirements for the travelling passage of the crawler potato harvester. This study provides an in-depth understanding of the tracked chassis of the potato combine harvester in hilly mountainous areas, with a view to providing reference for the design of tracked harvesters for other crops in hilly mountainous areas.
Green Development of Chinese Agriculture from the Perspective of Bidirectional Correlation
Authors: Yu Jinkuan , Sun Yao , Wei Feng
Published: September 2024

China’s agricultural green development exhibits spatial imbalances. To promote the coordinated green development of agriculture across various regions, this paper explores the evolution of spatial association networks and driving factors of agricultural green development from a bidirectional correlation perspective, using social network analysis and spatial econometric methods. This research indicates that China’s agricultural green efficiency has steadily improved, with a complex multi-threaded network structure. Although the spatial interaction and spillover effects of the overall network structure have increased, they remain relatively weak. The individual network structure shows significant regional imbalances. The spatial association network of agricultural green efficiency forms four major blocks, with sparse connections within the blocks but close connections between blocks, demonstrating strong spillover effects. Regarding the driving factors, the proportion of the primary industry, labor level, and R&D investment have significant spatial impacts, while the spatial impacts of human capital level, degree of openness, economic development level, and new quality productivity level are not significant. Therefore, we believe that it is necessary to establish the concept of coordinated green development in agriculture, fully leverage regional associations and spillover effects, and formulate differentiated policies to improve agricultural green efficiency.
Impact of Ecological Cognitive Bias on Pesticide Reduction by Natural Rubber Farmers in China: Insight from Price Insurance Satisfaction
Authors: Chen Donghui , Liu Jiyao , Zhang Desheng , Dong Zhixu , Xu Tao
Published: September 2024

Some natural rubber farmers mistakenly equate the ecological functions of rubber plantations with those of primary forests. This cognitive bias can hinder pesticide reduction efforts. Meanwhile, natural rubber farmers gain security through price insurance, which helps them adopt a long-term perspective on environmental protection, mitigating the negative impact of cognitive biases on pesticide use decisions. However, existing research often overlooks the influence of ecological cognitive bias on pesticide reduction and the moderating role of price insurance satisfaction. This study utilizes field survey data from Hainan and employs logit models and double machine learning models to empirically analyze the impact of ecological cognitive bias on pesticide reduction among natural rubber farmers. It further tests the underlying mechanisms using moderation models. The results indicate that (1) ecological cognitive bias negatively affects pesticide reduction among natural rubber farmers, and (2) price insurance satisfaction mitigates the impact of ecological cognitive bias on pesticide reduction. Based on these findings, the government should enhance education and training to raise ecological awareness among natural rubber farmers and reduce ecological cognitive biases. Disseminating knowledge about price insurance and establishing a comprehensive insurance system can secure farmers’ income and promote the rational use of pesticides.
Microplastics Can Inhibit Organic Carbon Mineralization by Influencing Soil Aggregate Distribution and Microbial Community Structure in Cultivated Soil: Evidence from a One-Year Pot Experiment
Authors: Chen Zonghai , Wan Quan , Zhou Pengyu , Li Haochen , Liu Yige , Lu Ying , Li Bo
Published: September 2024

Microplastics (MPs) pollution has become a global pollution problem, potentially affecting soil carbon cycling and structure stability in agricultural systems. However, the effects of MPs pollution on soil organic carbon fractions/transformation and soil aggregate stability remain unknown. Thus, a combination of one-year pot and short-term mineralized incubation experiments that involved a reference (CK, with no MPs), different concentrations (0.1, 1, and 2 w/w % polyethylene (PE)), and types (0.1 w/w % PE, polypropylene (PP), and polyvinyl chloride (PVC)) of MPs were carried out to investigate the effects on the soil aggregate stability and organic carbon mineralization after one year of adding MPs. The results showed that the size distribution of the soil partial aggregates varied significantly as affected by the MP concentration and type (p ˂ 0.05). Compared with 0.1% PE, significant increases in the MWD (mean weight diameter) and GMD (geometric mean diameter) of 2% PE of 27.22% and 32.73%, respectively, were detected. In addition, high concentrations (>1%) of PE significantly decreased the dissolved organic carbon (DOC) (p ˂ 0.05), whereas they significantly increased the stable carbon fractions including the particulate organic carbon (POC) and mineral-bound organic carbon (MOC) (p ˂ 0.01). Meanwhile, compared with the CK, both MP types and doses significantly decreased the soil organic carbon mineralization rate (SOCMR) and cumulative mineralization amount (CM) (p ˂ 0.001). Moreover, the MPs significantly increased the total PLFA (phospholipid fatty acid) by 261.9–438.8% (p ˂ 0.01), and the soil pH and total PLFA were the dominant factors that affected the SOCMR as affected by MPs. Thus, a high concentration (>1%) of PE significantly decreased the SOCMR by influencing the soil pH, TN, and macroaggregate (R>0.25) content and microbial community composition. This study provided evidence of the feedback of MPs pollution on soil C dynamic and aggregates in cultivated soil in South China.
Sensitivity Profile to Pyraclostrobin and Fludioxonil of Alternaria alternata from Citrus in Italy
Authors: Leonardi Giuseppa Rosaria , Quatra Greta La , Gusella Giorgio , Aiello Dalia , Vitale Alessandro , Camiletti Boris Xavier , Polizzi Giancarlo
Published: September 2024

Alternaria brown spot (ABS), caused by Alternaria alternata, is one of the main citrus diseases that causes heavy production losses and reductions in fruit quality worldwide. The application of chemical fungicides has a key role in the management of ABS. In this study, 48 isolates of A. alternata collected from citrus orchards since 2014 were tested in vitro for their sensitivity to pyraclostrobin and fludioxonil, the latter being temporarily registered in Italy since 2020. Pyraclostrobin sensitivity was determined using spore germination and mycelial growth assays. The effective concentration inhibiting 50% of fungal growth (EC50) was determined for each isolate. The sensitivity assays showed that the majority of A. alternata isolates tested were sensitive to pyraclostrobin. EC50 values of fludioxonil in a mycelial growth assay indicated that 100% of isolates were sensitive to this fungicide. The analysis of the cytochrome b gene showed that none of the 40 isolates with a different sensitivity profile had the G143A mutation, and the subgroup of 8 isolates analyzed by real-time PCR did not carry the G137R and F129L mutations. A subset of four more sensitive and two reduced-sensitive isolates was chosen to assess sensitivity on detached citrus leaves treated with pyraclostrobin at the maximum recommended label rate. Disease incidence and symptom severity were significantly reduced, with a small reduction reported in leaves inoculated with the reduced-sensitive isolates. Furthermore, there was no correlation between sensitivity and fitness parameters evaluated in vitro (mycelium growth and sporulation rate). These findings help the development of monitoring resistance programs and, consequently, set up effective anti-resistance strategies for managing ABS on citrus orchards.
Coexistence between Xylella fastidiosa Subsp. pauca and Susceptible Olive Plants in the Salento Peninsula (Southern Italy)
Authors: Bruno Giovanni Luigi
Published: September 2024

Olive Quick Decline Syndrome (OQDS) associated with Xylella fastidiosa subsp. pauca is one of the most destructive diseases of olive trees in the Salento Peninsula (Southern Italy), particularly on the cultivars Cellina di Nardò and Ogliarola Salentina. This study proposes the NuovOlivo protocol as a management strategy to permit coexistence between X. fastidiosa subsp. pauca and olive drupes and extra-virgin oil production. Thirty-two private olive orchards affected by OQDS and cultivated following the standard agronomic techniques in use in the area were surveyed during the 2019–2023 olive-growing seasons. Tested cultivars included Cellina di Nardò, Ogliarola Salentina, Coratina, Ascolana Tenera, Nociara, Leccino, and Bella di Cerignola. At the beginning of the protocol application, the susceptible plants showed OQDS symptom severity of 40–80% and did not produce olives or oil, while the resistant(?)/tolerant cultivars exhibited a 2–8% leaf scorch and a drupe production less than 1–2 kg/plant. After the removal of dry branches in January–February, plants were sprayed two times per year (preferably in March and October) with NuovOlivo®, a mixture of aqueous botanical extracts esterified in the presence of sodium hydroxide with vegetable oils and activated at the time of use with sodium bicarbonate. In all the orchards, a slow-release fertilizer was distributed, and weeds were controlled by mowing or chopping. Upon eventual appearance, the dry twigs were removed. Treated olive trees produced new vegetation, rebuilt their foliage, reduced OQDS symptoms, and turned out cluster inflorescence and drupes. The drupes yield was 6.67–51.36 kg per plant, with an average of 13.19% in extra-virgin olive oil (free acidity 0.01–0.2%). Plants used as controls showed OQDS symptoms and were unproductive, and newly formed shoots were desiccated. The proposed protocol promotes, supports, and restores new vegetation, flowers, fruits, and oil production of the treated olive plants affected by OQDS without losing susceptible olive plants. The Apulian landscape and economy, based on olive presence and production, could be also safeguarded.
Variations in Canine Behavioural Characteristics across Conventional Breed Clusters and Most Common Breed-Based Public Stereotypes
Authors: Peťková Barbara , Skurková Lenka , Florian Martin , Slivková Monika , Dudra Kasičová Zuzana , Kottferová Jana
Published: September 2024

Dog breeds are grouped based on scientific agreement, whether for traditional reasons or specific tasks during their domestication. Discrepancies may occur between public views of breed behaviour and actual evidence. This research aims to investigate differences in five behavioural traits (aggression towards people, aggression towards animals, fearfulness, responsiveness to training, and activity/excitability) by using the Dog Personality Questionnaire (DPQ) across six conventional groups/clusters of dog breeds (herding, hunting, guarding, companion dogs, potentially aggressive breeds, and mixed-breed dogs) and to assess hypotheses derived from common public presumptions. A cohort of 1309 dog owners sourced through diverse online platforms took part in the study. Contrary to stereotypes, the findings indicate that breeds labelled as “potentially aggressive” display lower levels of aggression compared to guarding breeds (χ2 (5) = 3.657, p = 0.041) and mixed-breeds (χ2 (5) = 3.870, p = 0.002). Additionally, mixed-breed dogs exhibited the highest levels of fearfulness among the six conventional clusters. In terms of aggression and gender, males demonstrated higher aggression levels towards both humans and animals compared to females (p = 0.001). These results challenge established assumptions and emphasise the necessity of evidence-based methodologies in the assessment of canine behaviour.
First Experimental Application of DNA-Layered Salmonid Alphavirus-Based Replicon Vaccine in Non-Salmonid Fish: Induced Early Semi-Specific Protection against Spring Viraemia of Carp Virus (SVCV) in Common Carp (Cyprinus carpio)
Authors: Abonyi Flóra , Eszterbauer Edit , Baska Ferenc , Hardy Tímea , Doszpoly Andor
Published: September 2024

Our study demonstrates the first application of the salmonid alphavirus-based replicon vector system (pSAV) as a DNA vaccine in a non-salmonid fish species, in common carp (Cyprinus carpio) against spring viraemia of carp virus (SVCV). SAV replicon encoding the glycoprotein of the SVCV was used as a DNA-layered plasmid, and its efficacy was compared with a previously described conventional DNA vaccine construct (pcDNA3.1 based vector) and with a control group (pcDNA3.1-empty-plasmid) in an SVCV challenge at a water temperature of 14 ± 1 °C. Vaccine prototypes were administered intramuscularly at a dose of 0.1 µg/g of fish (n = 25 per group). The DNA-layered SAV replicon resulted in 88% survival, compared to around 50% in all other groups. The DNA-layered pSAV vaccination induced the innate immune genes at the injection site, and increased IgM upregulation was also observed. Our preliminary results show that the SAV-based replicon construct may serve as a potential vaccine candidate for the protection of non-salmonid fish in the future provided that further clinical and field trials confirm its efficiency.
Hemp Seed (Cannabis sativa L.) Varieties: Lipids Profile and Antioxidant Capacity for Monogastric Nutrition
Authors: Rosso Elena , Armone Rosangela , Costale Annalisa , Meineri Giorgia , Chiofalo Biagina
Published: September 2024

The present research aimed to study the proximate composition, fatty acid profile, antiox-idant activity, total phenolic and N-trans-Caffeoyltyramine content of three distinct varieties of hemp seeds (Carmaenecta, Enectaliana and Enectarol, grown in a Mediterranean area (Central Italy), as feed in the diet of farm animals. Proximate composition was determined using the official methods of analyses; the fatty acid profile was determined by gas chromatography, total phenolic content (TPC) and the scavenging activity (DPPH• and ABTS•+) by the colorimetric method, and N-trans-Caffeoyltyramine content by HPLC analysis. The hemp seed Enectarol showed the highest total lipid content and the best antioxidant activity with the highest TPC, N-trans-Caffeoyltyramine content, and ABTS•+, and the lowest peroxidation index and DPPH•; Carmaenecta showed the best fatty acid profile and nutritional indices (atherogenic and thrombogenic indices and hypocholesterolemic/hypercholesterolemic ratio), and Enectaliana showed the highest crude protein and dietary fiber content. The differences observed in the chemical composition, fatty acid profile and antioxidant activity are because of the varieties, considering that all other growing conditions were the same. The results obtained suggest that hemp seed can be used as a source of lipid and protein in animal diets due to their valuable antioxidant activity and as a rich source of essential fatty acids.
Hormone-Driven Temperature Optimization for Elevated Reproduction in Goldfish (Carassius auratus) under Laboratory Conditions
Authors: Taheri-Khas Zeynab , Gharzi Ahmad , Vaissi Somaye , Heshmatzad Pouria , Kalhori Zahra
Published: September 2024

This study investigates the efficacy of hormone-induced artificial reproduction in goldfish (Carassius auratus) under controlled temperatures. Ovaprim injections significantly enhanced ovulation and sperm production compared to controls. Medium temperature (22 °C) produced the highest ovulation rates, fastest ovulation timing, and optimal sperm quality (motility and morphology) compared to high (28 °C) and low (16 °C) temperature groups. The low-temperature group exhibited reduced sperm motility duration and higher rates of sperm and larvae damage. The sperm volume of the high-temperature group was higher, but their post-injection survival rates were lower. Furthermore, the lowest spawning rate and low egg quality were noted in the high temperature. Cryopreservation using extender E4 (15% DMSO) exhibited superior post-thaw sperm motility and achieved higher fertilization rates. Fertilization rates, embryo development, and larval survival were all highest at the medium temperature. Larvae hatched from fresh sperm at medium temperature exhibited faster growth and fewer deformities. These findings suggest that hormone stimulation coupled with a medium temperature regimen is critical for successful artificial reproduction in goldfish. Cryopreservation with extender E4 holds promise for sperm banking; however, further optimization is necessary to improve fertilization success with thawed sperm. Future research could explore the influence of temperature on sperm physiology and refine cryopreservation protocols to enhance fertilization rates.
Dynamic Transcriptome Profile Analysis of Mechanisms Related to Melanin Deposition in Chicken Muscle Development
Authors: Ji Gaige , Zhang Ming , Ju Xiaojun , Liu Yifan , Shan Yanju , Tu Yunjie , Zou Jianmin , Shu Jingting , Li Hua , Zhao Weidong
Published: September 2024

The pectoral muscle is an important component of skeletal muscle. The blackness of pectoral muscles can directly affect the economic value of black-boned chickens. Although the genes associated with melanogenesis in mammals and birds have been thoroughly investigated, only little is known about the key genes involved in muscle hyperpigmentation during embryonic development. Here, we analyzed melanin deposition patterns in the pectoral muscle of Yugan black-boned chickens and compared differentially expressed genes (DEGs) between the muscles of Wenchang (non-black-boned chickens) and Yugan black-boned chickens on embryonic days 9, 13, 17, and 21. Melanin pigments were found to gradually accumulate in the muscle fibers over time. Using RNA-seq, there were 40, 97, 169, and 94 genes were identified as DEGs, respectively, between Yugan black-boned chicken muscles and Wenchang chickens at embryonic day 9, 13, 17, and 21 stages (fold change ≥2.0, false discovery rate (FDR) < 0.05). Thirteen DEGs, such as MSTRG.720, EDNRB2, TYRP1, and DCT, were commonly identified among the time points observed. These DEGs were mainly involved in pigmentation, melanin biosynthetic and metabolic processes, and secondary metabolite biosynthetic processes. Pathway analysis of the DEGs revealed that they were mainly associated with melanogenesis and tyrosine metabolism. Moreover, weighted gene co-expression network analysis (WGCNA) was used to detect core modules and central genes related to melanogenesis in the muscles of black-boned chickens. A total of 24 modules were identified. Correlation analysis indicated that one of them (the orange module) was positively correlated with muscle pigmentation traits (r > 0.8 and p < 0.001). Correlations between gene expression and L* values of the breast muscle were investigated in Yugan and Taihe black-boned chickens after hatching. The results confirmed that EDNRB2, GPNMB, TRPM1, TYR, and DCT expression levels were significantly associated with L* values (p < 0.01) in black-boned chickens (p < 0.05). Our results suggest that EDNRB2, GPNMB, TRPM1, TYR, and DCT are the essential genes regulating melanin deposition in the breast muscle of black-boned chickens. MSTRG.720 is a potential candidate gene involved in melanin deposition in the breast muscles of Yugan black-boned chickens.
Characterization of Two Novel Endolysins from Bacteriophage PEF1 and Evaluation of Their Combined Effects on the Control of Enterococcus faecalis Planktonic and Biofilm Cells
Authors: Wang Chen , Zhao Junxin , Lin Yunzhi , Lwin Su Zar Chi , El-Telbany Mohamed , Masuda Yoshimitsu , Honjoh Ken-ichi , Miyamoto Takahisa
Published: September 2024

Endolysin, a bacteriophage-derived lytic enzyme, has emerged as a promising alternative antimicrobial agent against rising multidrug-resistant bacterial infections. Two novel endolysins LysPEF1-1 and LysPEF1-2 derived from Enterococcus phage PEF1 were cloned and overexpressed in Escherichia coli to test their antimicrobial efficacy against multidrug-resistant E. faecalis strains and their biofilms. LysPEF1-1 comprises an enzymatically active domain and a cell-wall-binding domain originating from the NLPC-P60 and SH3 superfamilies, while LysPEF1-2 contains a putative peptidoglycan recognition domain that belongs to the PGRP superfamily. LysPEF1-1 was active against 89.86% (62/69) of Enterococcus spp. tested, displaying a wider antibacterial spectrum than phage PEF1. Moreover, two endolysins demonstrated lytic activity against additional gram-positive and gram-negative species pretreated with chloroform. LysPEF1-1 showed higher activity against multidrug-resistant E. faecalis strain E5 than LysPEF1-2. The combination of two endolysins effectively reduced planktonic cells of E5 in broth and was more efficient at inhibiting biofilm formation and removing biofilm cells of E. faecalis JCM 7783T than used individually. Especially at 4 °C, they reduced viable biofilm cells by 4.5 log after 2 h of treatment on glass slide surfaces. The results suggest that two novel endolysins could be alternative antimicrobial agents for controlling E. faecalis infections.
Establishment of an Antimicrobial Stewardship Program to Spare the Use of Oral Fluoroquinolones for Acute Uncomplicated Cystitis in Outpatients
Authors: Kato Tomoyuki , Nagasawa Masayuki , Tanaka Ippei , Seyama Yuka , Sekikawa Reiko , Yamada Shiori , Ishikawa Eriko , Kitajima Kento
Published: September 2024

The increase in fluoroquinolone (FQ)-resistant Escherichia coli (EC) is a serious global problem. In addition, much of acute uncomplicated cystitis (AUC) cases are caused by EC. FQs have been selected for the treatment of cystitis in outpatients, and there is concern about treatment failure. It is therefore necessary to select appropriate antimicrobials to spare FQs. However, there are few reported effective antimicrobial stewardship programs (ASPs) for outpatients. We aimed to establish the effective ASP for outpatients diagnosed with AUC caused by EC, to spare the use of FQs, and to explore optimal oral antimicrobials for AUC. The study subjects were outpatients treated for AUC caused by extended-spectrum β-lactamase-non-producing EC (non-ESBL-EC). Based on the antibiogram results, we recommended cefaclor (CCL) as the initial treatment for AUC, and educated clinical pharmacists who also worked together to advocate for CCL or cephalexin (CEX) prescriptions. FQ usages decreased, and cephalosporin (Ceph) prescriptions increased in all medical departments. The Ceph group (n = 114; CCL = 60, CEX = 54) in the non-FQ group had fewer treatment failures than the FQ group (n = 86) (12.3% vs. 31.4%). Cephs, including CCL and CEX, were effective treatments for AUC caused by non-ESBL-EC. Antimicrobial selection based on antibiogram results and the practice of an ASP in collaboration with clinical pharmacists were useful for optimizing antimicrobial therapy in outpatients.
Nail Lacquer Containing Origanum vulgare and Rosmarinus officinalis Essential Oils and Biogenic Silver Nanoparticles for Onychomycosis: Development, Characterization, and Evaluation of Antifungal Efficacy
Authors: Scandorieiro Sara , de Oliveira Natalia Rodrigues , de Souza Monique , de Castro-Hoshino Lidiane Vizioli , Baesso Mauro Luciano , Nakazato Gerson , Kobayashi Renata Katsuko Takayama , Panagio Luciano Aparecido , Lonni Audrey Alesandra Stinghen Garcia
Published: September 2024

Onychomycosis is a common fungal nail infection for which new antifungals are needed to overcome antimicrobial resistance and the limitations of conventional treatments. This study reports the development of antifungal nail lacquers containing oregano essential oil (OEO), rosemary essential oil (REO), and biogenic silver nanoparticles (bioAgNPs). The formulations (F) were tested against dermatophytes using agar diffusion, ex vivo nail infection, and scanning electron microscopy techniques. They were evaluated for their pharmacotechnical characteristics and by FTIR-PAS to assess permeation across the nail. F-OEO and F-OEO/bioAgNPs were promising candidates for the final nail lacquer formulation, as they permeated through the nail and showed antifungal efficacy against dermatophytes-contaminated nails after 5 days of treatment. Treated nails exhibited decreased hyphae and spores compared to the untreated control; the hyphae were atypically flattened, indicating loss of cytoplasmic content due to damage to the cytoplasmic membrane. The formulations were stable after centrifugation and thermal stress, maintaining organoleptic and physicochemical characteristics. Both F-OEO and F-OEO/bioAgNPs had pH compatible with the nail and drying times (59–90 s) within the reference for nail lacquer. For the first time, OEO and bioAgNPs were incorporated into nail lacquer, resulting in a natural and nanotechnological product for onychomycosis that could combat microbial resistance.
ST105 Lineage of MRSA: An Emerging Implication for Bloodstream Infection in the American and European Continents
Authors: Viana Alice Slotfeldt , Tótola Laís Pires do Valle , Figueiredo Agnes Marie Sá
Published: September 2024

Sequence-type 5 (ST5) of methicillin-resistant Staphylococcus aureus (MRSA), harboring the staphylococcal chromosomal cassette mec type IV (SCCmecIV), was first detected in Portugal. It emerged as a significant cause of healthcare-associated (HA) infection in pediatric units and was hence named the pediatric clone. Another ST5 lineage, which carries SCCmecII, also prevailed in the USA and Japan for multiple years. More recently, another MRSA lineage, ST105-SCCmecII, part of the evolution of clonal complex 5 (CC5) MRSA, has emerged as the cause of hospital-acquired bloodstream infection outbreaks in countries including Portugal, the USA, and Brazil. This article reviews studies on the epidemiology and evolution of these newly emerging pathogens. To this end, a search of PUBMED from inception to 2024 was performed to find articles reporting the occurrence of ST105 MRSA in epidemiologic studies. A second search was performed to find studies on MRSA, CC5, ST5, and SCCmecII. A search of PUBMED from 1999 to 2024 was also performed to identify studies on the genomics and evolution of ST5, CC5, and ST105 MRSA. Further studies were identified by analyzing the references of the previously selected articles from PUBMED. Most articles on ST105 MRSA were included in this review. Only articles written in English were included. Furthermore, only studies that used a reliable genotyping method (e.g., whole genome sequencing, or MLST) to classify the CC5 lineages were selected. The quality and selection of articles were based on the consensus assessment of the three authors in independent evaluations. In conclusion, ST105-SCCmecII is an emerging MRSA in several countries, being the second/third most important CC5 lineage, with a relatively high frequency in bloodstream infections. Of concern is the increased mortality from BSI in patients older than 15 years and the higher prevalence of ST105-SCCmecII in the blood of patients older than 60 years reported in some studies.
An ML-Based Solution in the Transformation towards a Sustainable Smart City
Authors: Rojek Izabela , Mikołajewski Dariusz , Dorożyński Janusz , Dostatni Ewa , Mreła Aleksandra
Published: September 2024

The rapid development of modern information technology (IT), power supply, communication and traffic information systems and so on is resulting in progress in the area of distributed and energy-efficient (if possible, powered by renewable energy sources) smart grid components securely connected to entire smart city management systems. This enables a wide range of applications such as distributed energy management, system health forecasting and cybersecurity based on huge volumes of data that automate and improve the performance of the smart grid, but also require analysis, inference and prediction using artificial intelligence. Data management strategies, but also the sharing of data by consumers, institutions, organisations and industries, can be supported by edge clouds, thus protecting privacy and improving performance. This article presents and develops the authors’ own concept in this area, which is planned for research in the coming years. The paper aims to develop and initially test a conceptual framework that takes into account the aspects discussed above, emphasising the practical aspects and use cases of the Social Internet of Things (SIoT) and artificial intelligence (AI) in the everyday lives of smart sustainable city (SSC) residents. We present an approach consisting of seven algorithms for the integration of large data sets for machine learning processing to be applied in optimisation in the context of smart cities.
Computational Shape Design Optimization of Femoral Implants: Towards Efficient Forging Manufacturing
Authors: Tuninetti Víctor , Fuentes Geovanni , Oñate Angelo , Narayan Sunny , Celentano Diego , García-Herrera Claudio , Menacer Brahim , Pincheira Gonzalo , Garrido César , Valle Rodrigo
Published: September 2024

Total hip replacement is one of the most successful orthopedic operations in modern times. Osteolysis of the femur bone results in implant loosening and failure due to improper loading. To reduce induced stress, enhance load transfer, and minimize stress, the use of Ti-6Al-4V alloy in bone implants was investigated. The objective of this study was to perform a three-dimensional finite element analysis (FEA) of the femoral stem to optimize its shape and analyze the developed deformations and stresses under operational loads. In addition, the challenges associated with the manufacturing optimization of the femoral stem using large strain-based finite element modeling were addressed. The numerical findings showed that the optimized femoral stem using Ti-6Al-4V alloy under the normal daily activities of a person presented a strains distribution that promote uniform load transfer from the proximal to the distal area, and provided a mass reduction of 26%. The stress distribution was found to range from 700 to 0.2 MPa in the critical neck area of the implant. The developed computational tool allows for improved customized designs that lower the risk of prosthesis loss due to stress shielding.
Spatial and Temporal Evolution Characteristics of the Ecosystem Service Value along the Beijing–Hangzhou Grand Canal
Authors: Xu Yuqing , Hu Di , He Handong , Zhang Zhuo , Bian Duo
Published: September 2024

The study of the spatiotemporal evolution characteristics of ecosystem service values (ESVs) is an important basis for the coordinated development of the regional nature, economy, and society and the optimization of the ecological environment. The ecological zone is an important component of the Beijing–Hangzhou Grand Canal cultural belt. Ecosystem services are a concrete manifestation of land use structure and function. A thorough study of the value of ecosystem services in areas along the Beijing–Hangzhou Grand Canal is important for promoting the long-term and stable sustainable development of the regional economy. Based on a revised equivalent factor table, this study selected land use data from 1991, 2006, and 2021 to analyze the temporal and spatial evolution characteristics of ESVs along the Beijing–Hangzhou Grand Canal. The results show that (1) the ESVs along the Grand Canal first increased and then decreased from 1991 to 2021. The reason for this is the change in land use along the Beijing–Hangzhou Grand Canal. Specifically, the conversion of land use types from farmland to water areas contributed to the increase in the value of ecosystem services, while the conversion of farmland and grassland into construction land led to a decrease in the service value of the region. (2) the value of individual ecosystem services along the Beijing–Hangzhou Grand Canal from 1991 to 2021 varied greatly. The ESV provided by hydrological regulation was the largest and the ESV provided by maintenance nutrients was the smallest. (3) the areas along the Beijing–Hangzhou Grand Canal exhibited a specific pattern in terms of the value of ecosystem services, with the regions centered in Beijing and Tianjin showing relatively low values, while the middle section of the Grand Canal demonstrated relatively high ESV. According to the spatial and temporal distribution characteristics and the leading factor for the changes in ESVs, appropriate policies can be formulated in respective regions to implement ecological protection and land use planning, thereby providing a reference for the adaptation and restoration strategies of the ecosystem along the Grand Canal.
Influence of Camera Placement on UGV Teleoperation Efficiency in Complex Terrain
Authors: Cieślik Karol , Krogul Piotr , Muszyński Tomasz , Przybysz Mirosław , Rubiec Arkadiusz , Typiak Rafał Kamil
Published: September 2024

Many fields, where human health and life are at risk, are increasingly utilizing mobile robots and UGVs (Unmanned Ground Vehicles). They typically operate in teleoperation mode (control based on the projected image, outside the operator’s direct field of view), as autonomy is not yet sufficiently developed and key decisions should be made by the man. Fast and effective decision making requires a high level of situational and action awareness. It relies primarily on visualizing the robot’s surroundings and end effectors using cameras and displays. This study aims to compare the effectiveness of three solutions of robot area imaging systems using the simultaneous transmission of images from three cameras while driving a UGV in complex terrain.
Uncertainty-Aware Federated Reinforcement Learning for Optimizing Accuracy and Energy in Heterogeneous Industrial IoT
Authors: Sagar A. S. M. Sharifuzzaman , Islam Muhammad Zubair , Haider Amir , Kim Hyung-Seok
Published: September 2024

The Internet of Things (IoT) technology has revolutionized various industries by allowing data collection, analysis, and decision-making in real time through interconnected devices. However, challenges arise in implementing Federated Learning (FL) in heterogeneous industrial IoT environments, such as maintaining model accuracy with non-Independent and Identically Distributed (non-IID) datasets and straggler IoT devices, ensuring computation and communication efficiency, and addressing weight aggregation issues. In this study, we propose an Uncertainty-Aware Federated Reinforcement Learning (UA-FedRL) method that dynamically selects epochs of individual clients to effectively manage heterogeneous industrial IoT devices and improve accuracy, computation, and communication efficiency. Additionally, we introduce the Predictive Weighted Average Aggregation (PWA) method to tackle weight aggregation issues in heterogeneous industrial IoT scenarios by adjusting the weights of individual models based on their quality. The UA-FedRL addresses the inherent complexities and challenges of implementing FL in heterogeneous industrial IoT environments. Extensive simulations in complex IoT environments demonstrate the superior performance of UA-FedRL on both MNIST and CIFAR-10 datasets compared to other existing approaches in terms of accuracy, communication efficiency, and computation efficiency. The UA-FedRL algorithm attain an accuracy of 96.83% on the MNIST dataset and 62.75% on the CIFAR-10 dataset, despite the presence of 90% straggler IoT devices, attesting to its robust performance and adaptability in different datasets.
Analysis of Flow Field Characteristics in the Three-Phase Jet Fire Monitor Head
Authors: Ge Hongen , Zhang Liye , Zhang Xin , Jiao Longfei
Published: September 2024

To enhance the jet performance of the three-phase jet fire monitor (TPJFM), an analysis was conducted on the internal flow field (IFF) characteristics of the monitor head. Using the volume of fluid method, the impact of key structural parameters, such as the powder-pipe bending angle and the supporting blade length, on the turbulent kinetic energy (TKE) of the IFF, the velocity distribution uniformity at the nozzle outlet, and the pressure drop (PD) between the inlet and outlet of the internal flow field, was analyzed. The study revealed that increasing the bending angle of the powder pipe will lead to a significant improvement in the uniformity of velocity distribution in the IFF. Extending the supporting blade length helps reduce the average TKE at the nozzle outlet but has a minimal impact on the velocity distribution uniformity and the PD between the inlet and outlet. Reasonable design of the distance between the supporting blades and the bending section of the powder pipe can improve the IFF characteristics, reducing local pressure losses and peak TKE. The research results can effectively improve the IFF characteristics, enhance jet performance, and provide theoretical basis and technical support for the design and optimization of the TPJFM.
The Structural Modification of Jackfruit Leaf Proteins (Artocarpus heterophyllus Lam.) by High-Intensity Ultrasound Alters Their Techno-Functional Properties and Antioxidant Capacity
Authors: Ragazzo-Calderón Frida Zoé , Iñiguez-Moreno Maricarmen , Calderón-Santoyo Montserrat , Ragazzo-Sánchez Juan Arturo
Published: September 2024

(1) Background: Jackfruit leaves (Artocarpus heterophyllus Lam.) are rich in proteins but are under-utilized in the food industry due to their poor sensory properties and low solubility. High-intensity ultrasound (HIU) can enhance protein solubility by disrupting internal interactions and altering protein structures, making them more suitable for industrial applications. (2) Methods: This study aimed to modify the structure of jackfruit leaf proteins using HIU at different powers (600, 840, and 1080 W) and treatment times (10, 15, and 20 min). This research also characterized the amino acid composition and the techno-functional and antioxidant properties of the modified proteins. (3) Results: The HIU treatments significantly improved the foaming capacity and enhanced the emulsion stability within the proteins treated at 1080 W for 15 min, which showed a monomodal size distribution profile. Additionally, the modified proteins exhibited a higher antioxidant capacity compared to the native protein. (4) Conclusions: These findings suggest that structurally modified proteins from jackfruit leaves could be directly utilized in the formulation of emulsions or as foam stabilizers, offering added benefits to consumers due to their significant antioxidant properties.