bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats
Authors: Chao Wen-Wan , Chen Shu-Ju , Peng Hui-Chen , Liao Jiunn-Wang , Chou Su-Tze
Published: July 2019

(1) Background: Graptopetalum paraguayense E. Walther is a traditional Chinese herbal medicine. In our previous study, 50% ethanolic G. paraguayense extracts (GE50) demonstrated good antioxidant activity. (2) Methods: To investigate the hepatoprotective effects of GE50 on ethanol and carbon tetrachloride (CCl4) co-induced hepatic damage in rats, Sprague–Dawley rats were randomly divided into five groups (Control group; GE50 group, 0.25 g/100 g BW; EC group: Ethanol + CCl4, 1.25 mL 50% ethanol and 0.1 mL 20% CCl4/100 g BW; EC + GE50 group: Ethanol + CCl4 + GE50; EC + silymarin group: ethanol + CCl4 + silymarin, 20 mg/100 g BW) for six consecutive weeks. (3) Results: Compared with the control group, EC group significantly elevated the serum aspartate aminotransferase (AST), alanine aminitransferase (ALT), and lactate dehydrogenase (LDH). However, GE50 or silymarin treatment effectively reversed these changes. GE50 had a significant protective effect against ethanol + CCl4 induced lipid peroxidation and increased the levels of glutathione (GSH), vitamin C, E, total antioxidant status (TAS), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferases (GST). Furthermore, in EC focal group, slight fat droplet infiltration was observed in the livers, while in the GE50 or silymarin treatment groups, decreased fat droplet infiltration. HPLC phytochemical profile of GE50 revealed the presence of gallic acid, flavone, genistin, daidzin, and quercetin. (4) Conclusions: The hepatoprotective activity of GE50 is proposed to occur through the synergic effects of its chemical component, namely, gallic acid, flavone, genistin, daidzin, and quercetin. Hence, G. paraguayense can be used as a complementary and alternative therapy in the prevention of alcohol + CCl4-induced liver injury.
Applying Selective Laser Melting to Join Al and Fe: An Investigation of Dissimilar Materials
Authors: Nguyen Dinh-Son , Park Hong-Seok , Lee Chang-Myung
Published: July 2019

Combining aluminum and steel is a major goal of automobile manufacturers and other industries because the hybrid material reduces the weight of components. However, differences in chemical properties, thermal expansion, and physical characteristics of aluminum and steel are barriers to achieving this goal. In this article, selective laser melting (SLM), which is widely used in industrial fields, was applied to join dissimilar materials by printing aluminum on a steel substrate. Defects of joining during the SLM process, characteristics of the intermetallic reaction layer, and the effects of the process parameters were investigated. The analysis indicates that flake behavior could affect the quality of joining. The phases of the intermetallic layer found in this study were in agreement with other research, but the morphology of the layer was much different. A formula to estimate the join quality in terms of density energy is proposed. The results indicate that the SLM process is a promising method to manufacture a hybrid material.
Effect of Doping Microcapsules on Typical Electrical Performances of Self-Healing Polyethylene Insulating Composite
Authors: Wang Youyuan , Li Yudong , Zhang Zhanxi , Zhang Yanfang
Published: July 2019

Polyethylene cables, as important transmission equipment of modern power grid, would inevitably be slightly damaged, which seriously threatens the safety of the power supply. This paper has pioneered the preparation and typical performances of a self-healing polyethylene insulating composite. The self-healing performance to structural damage was verified by tests of electrical and mechanical damage. The effect mechanism of doping microcapsules on the electrical performance of polyethylene was emphatically analyzed. The results show that in appropriate conditions (such as 60 °C/30 min), the composite can not only repair the electrical tree and scratches, but also restore the insulation strength of damaged area. The effect of doping microcapsules on the electrical performances of polyethylene, such as breakdown strength, volumetric resistivity, dielectric properties, and space charge characteristics, are mainly related to impurity and the interface of microcapsule. Polarization and ionization of impurities can reduce the electrical performance of polyethylene. The interface not only improves the microstructure of polyethylene (such as how the heterogeneous nucleation effect increases the number of crystal regions, and the anchoring effect enhances the stability of amorphous regions), but also increases the charge traps. Moreover, the microstructure and charge trap can affect the characteristics of carrier transport, material polarization, and space charge accumulation, thus improving the electrical performance of polyethylene. In addition, the important electrical performance of the composite can meet the basic application requirements of polyethylene insulating material, which has good application prospects.
Vocal Pitch Discrimination in Children with and without Vocal Fold Nodules
Authors: Heller Murray Elizabeth S. , Hseu Anne F. , Nuss Roger C. , Harvey Woodnorth Geralyn , Stepp Cara E.
Published: July 2019

Vocal pitch discrimination abilities were compared in sixteen children with vocal fold nodules (CwVN) and sixteen matched controls with typical voices (CwTV). Vocal pitch discrimination was also evaluated in thirty-five vocally healthy children and twenty adults to examine potential changes as a function of maturation. CwTV were categorized as either younger (N = 15, 5.6–7.7 years) or older (N = 20, 8.2–11.7 years). Participants completed two-alternative, forced choice listening tasks in which they judged whether pairs of sustained /ɑ/ tokens were different in pitch. Each pair consisted of a base token with a fundamental frequency (fo) of 216.2 Hz and a test token with a fo that was adaptively modified, according to the participant’s prior judgments. There were no significant differences in pitch discrimination abilities between CwVN and CwTV. Pitch discrimination abilities were significantly poorer in younger and older CwTV as compared to adults. Additionally, younger CwTV had significantly poorer discrimination abilities than older CwTV. Findings from this study suggest that CwVN do not have differences in pitch discrimination abilities, yet, therapies designed for CwVN should consider this developmental trend in perceptual abilities.
Advances in the Study of the Behavior of Full-Depth Reclamation (FDR) with Cement
Authors: Gonzalo-Orden Hernán , Linares-Unamunzaga Alaitz , Pérez-Acebo Heriberto , Díaz-Minguela Jesús
Published: July 2019

Road maintenance and rehabilitation are expected to meet modern society’s demands for sustainable development. Full-depth reclamation with cement as a binder is closely linked to the concept of sustainability. In addition to the environmental benefits of reusing the existing pavement as aggregate, this practice entails significant technical and economic advantages. In Spain, in the absence of tests specifically designed to determine the behavior of recycled pavements stabilized with cement, these materials are treated as soil-cement or cement-bound granular material. This assumption is not entirely accurate, because this recycled pavement contains some bituminous elements that reduce its stiffness. This study aimed to obtain the relationships between flexural strength (FS) and the parameters that describe the pavement behavior (long-term unconfined compressive strength (UCS) and indirect tensile strength (ITS)) and compare the findings with the relationships between these parameters in soil-cement and cement-bound granular materials. The results showed that the similar behavior hypothesis is not entirely accurate for recycled pavements stabilized with cement, because they have lower strength values—although, this is not necessarily an indication of poorer performance.
Lavandula Luisieri and Lavandula Viridis Essential Oils as Upcoming Anti-Protozoal Agents: A Key Focus on Leishmaniasis
Authors: Machado Marisa , Martins Natália , Salgueiro Lígia , Cavaleiro Carlos , Sousa Maria C.
Published: July 2019

Background and objectives: Leishmania species is the causative agent of leishmaniasis, a broad-spectrum clinical condition that can even be life-threatening when neglected. Current therapeutic strategies, despite beings highly cost-effective, have been increasingly associated with the appearance of drug-resistant microorganisms. Thus, an increasing number of thorough studies are needed towards upcoming drug discovery. This study aims to reveal the anti-protozoa activity of Lavandula luisieri and Lavandula viridis essential oils (EO) and their main components (1,8-cineole, linalool, and borneol). Materials and Methods: L. luisieri and L. viridis EO and their main components’ leishmanicidal effects were tested in vitro against Leishmania infantum, Leishmania major, and Leishmania tropica strains. Cell viability effects were estimated by using the tetrazolium-dye (MTT) colorimetric method, morphological changes were assessed by scanning electron microscopy (SEM) and ultrastructural investigation by transmission electronic microscopy (TEM). Phosphatidylserine externalization, mitochondrial membrane potential (MMP), and cathepsin D activity assessment were also carried out. Finally, cytotoxic activity of the studied matrices was also determined in mammalian cells. Results: Plant-studied EO exhibited prominent anti-Leishmania effects (IC50 = 31–263 µg/mL), with L. luisieri being the most active one. At concentrations corresponding to IC50 values, EO-exposed L. infantum promastigotes suffered marked ultrastructural modifications. The presence of aberrant-shaped cells, mitochondrial and kinetoplast swelling, and autophagosomal structures were the most common evidenced changes. L. luisieri EO exerted its leishmanicidal activity through different mechanisms, but mainly through unleashing apoptosis. Phosphatidylserine externalization, mitochondrial membrane potential loss, and cell-cycle arrest at G(0)/G(1) phase were the most remarkable apoptosis-mediated aspects. Inhibition of cathepsin D activity was also observed. No toxic effects were found on macrophage cells. Conclusions: L. luisieri seems to be an upcoming source of bioactive molecules for leishmaniasis control and to find leading molecules for new drugs formulation against Leishmania infections.
Experimental Investigation of Force Response, Efficiency, and Wear Behaviors of Polycrystalline Diamond Rock Cutters
Authors: Xiao Huaping , Liu Shuhai , Tan Kaiwen
Published: July 2019

Polycrystalline diamond compact (PDC) cutters are the most extensively used tool for rock drilling in superdeep oil and gas exploration, in which the air drilling technology without drilling fluid is highly promoted. This study examined the performance of PDC cutters in air drilling, including their friction angle, cutting force, specific energy, and wear behaviors, using a home-made testing apparatus and a commercial tribometer. It also investigated the dependence of cutting force on cutting depth and back rake angle. Results obtained in both dry conditions and in drilling fluid media were compared, and a tentative explanation to the observed differences was brought about by these two environments.
Hyperspectral Image Inpainting Based on Robust Spectral Dictionary Learning
Authors: Song Xiaorui , Wu Lingda
Published: July 2019

To address the problems of defective pixels and strips in hyperspectral images affecting subsequent processing and applications, we modeled the hyperspectral image (HSI) inpainting problem as a sparse signal reconstruction problem with incomplete observations using the theory of sparse representation, and proposed an HSI inpainting algorithm based on spectral dictionary learning. First, we studied the HSI observation model under the assumption of additive noise. We subsequently proposed a new algorithm for constructing a spectral dictionary directly from hyperspectral data by introducing an online learning optimization method and performing dictionary learning using a robust function. Afterwards, the image was sparsely encoded by applying the variable decomposition and augmented Lagrangian sparse regression method. Finally, the inpainted HSI was obtained by sparse reconstruction. The experimental results showed that compared with the existing algorithms, the algorithm proposed herein could effectively inpaint the defective HSI under different noise conditions with a shorter calculation time than those of existing methods and other dictionary learning inpainting algorithms.
Optimal Maintenance Thresholds to Perform Preventive Actions by Using Multi-Objective Evolutionary Algorithms
Authors: Goti Aitor , Oyarbide-Zubillaga Aitor , Alberdi Elisabete , Sanchez Ana , Garcia-Bringas Pablo
Published: July 2019

Maintenance has always been a key activity in the manufacturing industry because of its economic consequences. Nowadays, its importance is increasing thanks to the “Industry 4.0” or “fourth industrial revolution”. There are more and more complex systems to maintain, and maintenance management must gain efficiency and effectiveness in order to keep all these devices in proper conditions. Within maintenance, Condition-Based Maintenance (CBM) programs can provide significant advantages, even though often these programs are complex to manage and understand. For this reason, several research papers propose approaches that are as simple as possible and can be understood by users and modified by experts. In this context, this paper focuses on CBM optimization in an industrial environment, with the objective of determining the optimal values of preventive intervention limits for equipment under corrective and preventive maintenance cost criteria. In this work, a cost-benefit mathematical model is developed. It considers the evolution in quality and production speed, along with condition based, corrective and preventive maintenance. The cost-benefit optimization is performed using a Multi-Objective Evolutionary Algorithm. Both the model and the optimization approach are applied to an industrial case.
Flocculation Harvesting Techniques for Microalgae: A Review
Authors: Matter Ibrahim A. , Bui Vu Khac Hoang , Jung Mikyoung , Seo Jung Yoon , Kim Young-Eun , Lee Young-Chul , Oh You-Kwan
Published: July 2019

Microalgae have been considered as one of the most promising biomass feedstocks for various industrial applications such as biofuels, animal/aquaculture feeds, food supplements, nutraceuticals, and pharmaceuticals. Several biotechnological challenges associated with algae cultivation, including the small size and negative surface charge of algal cells as well as the dilution of its cultures, need to be circumvented, which increases the cost and labor. Therefore, efficient biomass recovery or harvesting of diverse algal species represents a critical bottleneck for large-scale algal biorefinery process. Among different algae harvesting techniques (e.g., centrifugation, gravity sedimentation, screening, filtration, and air flotation), the flocculation-based processes have acquired much attention due to their promising efficiency and scalability. This review covers the basics and recent research trends of various flocculation techniques, such as auto-flocculation, bio-flocculation, chemical flocculation, particle-based flocculation, and electrochemical flocculation, and also discusses their advantages and disadvantages. The challenges and prospects for the development of eco-friendly and economical algae harvesting processes have also been outlined here.
Decarbonizing Space and Water Heating in Temperate Climates: The Case for Electrification
Authors: Sheikh Imran , Callaway Duncan
Published: July 2019

In order to meet ambitious carbon reduction goals, direct combustion of fossil fuels in homes will need to largely cease. The largest portion of this reduction will likely come from energy efficiency, but efficiency alone will not be sufficient. In this paper we look specifically at California and build the case for why energy efficiency with electrification of heating is the most likely path to achieve the large carbon emission reduction needed from this sector. We examine alternative decarbonization strategies, such as solar thermal, biogas, synthetic natural gas and electrification and show why electrification is the most promising path. We evaluate these options across the dimensions of scale, cost, and suitability. We find that, while electrification has the potential to serve all heating loads, the other low-carbon options may serve only 2–70% of loads. We also expect that electrification could reduce emissions from this sector at a cost 25–90+% less than other options.
Observed Trends in Thermal Stress at European Cities with Different Background Climates
Authors: Founda Dimitra , Pierros Fragiskos , Katavoutas George , Keramitsoglou Iphigenia
Published: July 2019

Intensification of extreme temperatures combined with other socioeconomic factors may exacerbate human thermal risk. The disastrous impacts of extreme weather during the last two decades demonstrated the increased vulnerability of populations even in developed countries from Europe, which are expected to efficiently manage adverse weather. The study aims to assess trends in the exposure of European populations to extreme weather using updated historical climatic data in large European cities of different local climates and a set of climatic and bioclimatic indices. Colder cities experience higher warming rates in winter (exceeding 1 °C/decade since the mid-1970s) and warmer cities in summer. Hot extremes have almost tripled in most cities during the last two or three decades with simultaneous advancing of hot weather, while northernmost cities have experienced an unprecedented increase in the heat waves frequency only during the last decade. Bioclimatic indices suggested a robust tendency towards less cold-related stress (mainly in cold cities) and more heat-related stress in all cities. A doubling or tripling in the frequency of heat-related ‘great discomfort’ was found in southern cities, while in the cities of northern Europe, heat-related ‘discomfort’ conditions are becoming increasingly more frequent and have nearly quadrupled during the last decade.
Extraction of Peak Velocity Profiles from Doppler Echocardiography Using Image Processing
Authors: Taebi Amirtahà , Sandler Richard H. , Kakavand Bahram , Mansy Hansen A.
Published: July 2019

The objective of this study is to extract positive and negative peak velocity profiles from Doppler echocardiographic images. These profiles are currently estimated using tedious manual approaches. Profiles can be used to establish realistic boundary conditions for computational hemodynamic studies and to estimate cardiac time intervals, which are of clinical utility. In the current study, digital image processing algorithms that rely on intensity calculations and two different thresholding methods were proposed and tested. Image intensity histograms were used to guide threshold choices, which were selected such that the resulting velocity profiles appropriately represent Doppler shift envelopes. The resulting peak velocity profiles contained artifacts in the form of sudden velocity changes and possible outliers. To reduce these artifacts, image smoothing using a moving average process was then implemented. Bland–Altman analysis suggested good agreement between the two thresholding methods. Artifacts decreased when image smoothing was performed. Results also suggested that one thresholding method tended to provide the lower limit (i.e., underestimate) of velocities, while the second tended to provide the velocity upper limit (i.e., overestimate). Combining estimates from both methods appeared to provide a smoother peak velocity profile estimate. The proposed automated approach may be useful for objective estimation of peak velocity profiles, which may be helpful for computational hemodynamic studies and may increase the efficiency of current clinical diagnostic tools.
Cell Intrinsic and Extrinsic Mechanisms of Caveolin-1-Enhanced Metastasis
Authors: Campos America , Burgos-Ravanal Renato , González María , Huilcaman Ricardo , Lobos González Lorena , Quest Andrew
Published: July 2019

Caveolin-1 (CAV1) is a scaffolding protein with a controversial role in cancer. This review will initially discuss earlier studies focused on the role as a tumor suppressor before elaborating subsequently on those relating to function of the protein as a promoter of metastasis. Different mechanisms are summarized illustrating how CAV1 promotes such traits upon expression in cancer cells (intrinsic mechanisms). More recently, it has become apparent that CAV1 is also a secreted protein that can be included into exosomes where it plays a significant role in determining cargo composition. Thus, we will also discuss how CAV1 containing exosomes from metastatic cells promote malignant traits in more benign recipient cells (extrinsic mechanisms). This ability appears, at least in part, attributable to the transfer of specific cargos present due to CAV1 rather than the transfer of CAV1 itself. The evolution of how our perception of CAV1 function has changed since its discovery is summarized graphically in a time line figure.
Diagnosis and Management of Progressive Multiple Sclerosis
Authors: Macaron Gabrielle , Ontaneda Daniel
Published: July 2019

Multiple sclerosis is a chronic autoimmune disease of the central nervous system that results in varying degrees of disability. Progressive multiple sclerosis, characterized by a steady increase in neurological disability independently of relapses, can occur from onset (primary progressive) or after a relapsing–remitting course (secondary progressive). As opposed to active inflammation seen in the relapsing–remitting phases of the disease, the gradual worsening of disability in progressive multiple sclerosis results from complex immune mechanisms and neurodegeneration. A few anti-inflammatory disease-modifying therapies with a modest but significant effect on measures of disease progression have been approved for the treatment of progressive multiple sclerosis. The treatment effect of anti-inflammatory agents is particularly observed in the subgroup of patients with younger age and evidence of disease activity. For this reason, a significant effort is underway to develop molecules with the potential to induce myelin repair or halt the degenerative process. Appropriate trial methodology and the development of clinically meaningful disability outcome measures along with imaging and biological biomarkers of progression have a significant impact on the ability to measure the efficacy of potential medications that may reverse disease progression. In this issue, we will review current evidence on the physiopathology, diagnosis, measurement of disability, and treatment of progressive multiple sclerosis.
Bioisosteres of Carbohydrate Functional Groups in Glycomimetic Design
Authors: Hevey Rachel
Published: July 2019

The aberrant presentation of carbohydrates has been linked to a number of diseases, such as cancer metastasis and immune dysregulation. These altered glycan structures represent a target for novel therapies by modulating their associated interactions with neighboring cells and molecules. Although these interactions are highly specific, native carbohydrates are characterized by very low affinities and inherently poor pharmacokinetic properties. Glycomimetic compounds, which mimic the structure and function of native glycans, have been successful in producing molecules with improved pharmacokinetic (PK) and pharmacodynamic (PD) features. Several strategies have been developed for glycomimetic design such as ligand pre-organization or reducing polar surface area. A related approach to developing glycomimetics relies on the bioisosteric replacement of carbohydrate functional groups. These changes can offer improvements to both binding affinity (e.g., reduced desolvation costs, enhanced metal chelation) and pharmacokinetic parameters (e.g., improved oral bioavailability). Several examples of bioisosteric modifications to carbohydrates have been reported; this review aims to consolidate them and presents different possibilities for enhancing core interactions in glycomimetics.
Dynamic Displacement of an Aluminum Frame Using Close Range Photogrammetry
Authors: Ghaychi Afrouz Setareh , Razavi Mohammad Reza , Pourkand Ashkan , Mara Dias Wilson Claudia
Published: July 2019

Dynamic displacement measurement of objects can be challenging due to the limitations of conventional methods and pricey instrumentation of unconventional methods, such as laser scanners. In this research, Close Range Photogrammetry (CRP) is used as an affordable non-contact method to measure 3D dynamic displacements. It is proposed as a reliable alternative to traditional dynamic deformation measurement methods such as displacement sensors or accelerometers. For this purpose, dynamic displacements of a three-dimensional one-story building frame model on a one-dimensional shake table are determined by using the traditional method of attached accelerometer and CRP. The results of the CRP method are compared with the results of the traditional methods as well as numerical models. The results show a good agreement which evidences the reliability of the CRP with regular cameras.
APOBEC3B Gene Expression in Ductal Carcinoma In Situ and Synchronous Invasive Breast Cancer
Authors: Sieuwerts Anieta M. , Doebar Shusma C. , de Weerd Vanja , Verhoef Esther I. , Beauford Corine M. , Agahozo Marie C. , Martens John W.M. , van Deurzen Carolien H.M.
Published: July 2019

The underlying mechanism of the progression of ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive breast cancer (IBC), has yet to be elucidated. In IBC, Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3B (APOBEC3B) is upregulated in a substantial proportion of cases and is associated with higher mutational load and poor prognosis. However, APOBEC3B expression has never been studied in DCIS. We performed mRNA expression analysis of APOBEC3B in synchronous DCIS and IBC and surrounding normal cells. RNA was obtained from 53 patients. The tumors were categorized based on estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her2) and phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA) mutation status. APOBEC3B mRNA levels were measured by RT-qPCR. The expression levels of paired DCIS and adjacent IBC were compared, including subgroup analyses. The normal cells expressed the lowest levels of APOBEC3B. No differences in expression were found between DCIS and IBC. Subgroup analysis showed that APOBEC3B was the highest in the ER subgroups of DCIS and IBC. While there was no difference in APOBEC3B between wild-type versus mutated PIK3CA DCIS, APOBEC3B was higher in wild-type versus PIK3CA-mutated IBC. In summary, our data show that APOBEC3B is already upregulated in DCIS. This suggests that APOBEC3B could already play a role in early carcinogenesis. Since APOBEC3B is a gain-of-function mutagenic enzyme, patients could benefit from the therapeutic targeting of APOBEC3B in the early non-invasive stage of breast cancer.
Persistent Organic Pollutants and Breast Cancer: A Systematic Review and Critical Appraisal of the Literature
Authors: Ennour-Idrissi Kaoutar , Ayotte Pierre , Diorio Caroline
Published: July 2019

Persistent organic pollutants (POPs) bioaccumulate in the food chain and have been detected in human blood and adipose tissue. Experimental studies demonstrated that POPs can cause and promote growth of breast cancer. However, inconsistent results from epidemiological studies do not support a causal relationship between POPs and breast cancer in women. To identify individual POPs that are repeatedly found to be associated with both breast cancer incidence and progression, and to demystify the observed inconsistencies between epidemiological studies, we conducted a systematic review of 95 studies retrieved from three main electronic databases. While no clear pattern of associations between blood POPs and breast cancer incidence could be drawn, POPs measured in breast adipose tissue were more clearly associated with higher breast cancer incidence. POPs were more consistently associated with worse breast cancer prognosis whether measured in blood or breast adipose tissue. In contrast, POPs measured in adipose tissue other than breast were inversely associated with both breast cancer incidence and prognosis. Differences in biological tissues used for POPs measurement and methodological biases explain the discrepancies between studies results. Some individual compounds associated with both breast cancer incidence and progression, deserve further investigation.
Downregulation of SHIP2 by Hepatitis B Virus X Promotes the Metastasis and Chemoresistance of Hepatocellular Carcinoma through SKP2
Authors: Su Kuo-Jung , Yu Yung-Luen
Published: July 2019

Hepatitis B virus (HBV)-encoded X protein (HBx) plays an important role in the development of hepatocellular carcinoma (HCC). The protein SH2 domain containing inositol 5-phosphatase 2 (SHIP2) belongs to the family of enzymes that dephosphorylate the 5 position of PI(3,4,5)P3 to produce PI(3,4)P2. Expression of SHIP2 has been associated with several cancers including HCC. However, its role in the development of HBV-related HCC remains elusive. In this study, we performed tissue microarray analysis using 49 cases of HCC to explore SHIP2 expression changes and found that SHIP2 was downregulated in HBV-positive HCC. In addition, S-phase kinase-associated protein 2 (SKP2), a component of the E3 ubiquitin–ligase complex, was increased in HCC cell lines that overexpressed HBx, which also showed a notable accumulation of polyubiquitinated SHIP2. Moreover, HCC cells with silenced SHIP2 had increased expression of mesenchymal markers, which promotes cell migration, enhances glucose uptake, and leads to resistance to the chemotherapy drug (5-Fluorouracil, 5-FU). Taken together, our results demonstrate that HBx downregulates SHIP2 through SKP2 and suggest a potential role for SHIP2 in HBx-mediated HCC migration.
Liquid Biopsy in Lung Cancer Screening: The Contribution of Metabolomics. Results of A Pilot Study
Authors: Singhal Sandeep , Rolfo Christian , Maksymiuk Andrew W. , Tappia Paramjit S. , Sitar Daniel S. , Russo Alessandro , Akhtar Parveen S. , Khatun Nazrina , Rahnuma Parveen , Rashiduzzaman Ahmed , Ahmed Bux Rashid , Huang Guoyu , Ramjiawan Bram
Published: July 2019

Background: Lung cancer is the most common cause of cancer-related deaths worldwide. Early diagnosis is crucial to increase the curability chance of the patients. Low dose CT screening can reduce lung cancer mortality, but it is associated with several limitations. Metabolomics is a promising technique for cancer diagnosis due to its ability to provide chemical phenotyping data. The intent of our study was to explore metabolomic effects and profiles of lung cancer patients to determine if metabolic perturbations in the SSAT-1/polyamine pathway can distinguish between healthy participants and lung cancer patients as a diagnostic and treatment monitoring tool. Patients and Methods: Plasma samples were collected as part of the SSAT1 Amantadine Cancer Study. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify and quantify metabolite concentrations in lung cancer patient and control samples. Standard statistical analyses were performed to determine whether metabolite concentrations could differentiate between healthy subjects and lung cancer patients, as well as risk prediction modeling applied to determine whether metabolic profiles could provide an indication of cancer progression in later stage patients. Results: A panel consisting of 14 metabolites, which included 6 metabolites in the polyamine pathway, was identified that correctly discriminated lung cancer patients from controls with an area under the curve of 0.97 (95% CI: 0.875-1.0). Conclusion: When used in conjunction with the SSAT-1/polyamine pathway, these metabolites may provide the specificity required for diagnosing lung cancer from other cancer types and could be used as a diagnostic and treatment monitoring tool.
Mesenchymal Stem Cell Migration and Tissue Repair
Authors: Fu Xiaorong , Liu Ge , Halim Alexander , Ju Yang , Luo Qing , Song Guanbin
Published: July 2019

Mesenchymal stem cells (MSCs) are multilineage cells with the ability to self-renew and differentiate into a variety of cell types, which play key roles in tissue healing and regenerative medicine. Bone marrow-derived mesenchymal stem cells (BMSCs) are the most frequently used stem cells in cell therapy and tissue engineering. However, it is prerequisite for BMSCs to mobilize from bone marrow and migrate into injured tissues during the healing process, through peripheral circulation. The migration of BMSCs is regulated by mechanical and chemical factors in this trafficking process. In this paper, we review the effects of several main regulatory factors on BMSC migration and its underlying mechanism; discuss two critical roles of BMSCs—namely, directed differentiation and the paracrine function—in tissue repair; and provide insight into the relationship between BMSC migration and tissue repair, which may provide a better guide for clinical applications in tissue repair through the efficient regulation of BMSC migration.
Macrophage S1PR1 Signaling Alters Angiogenesis and Lymphangiogenesis During Skin Inflammation
Authors: Syed Shahzad Nawaz , Raue Rebecca , Weigert Andreas , von Knethen Andreas , Brüne Bernhard
Published: July 2019

The bioactive lipid sphingosine-1-phosphate (S1P), along with its receptors, modulates lymphocyte trafficking and immune responses to regulate skin inflammation. Macrophages are important in the pathogenesis of psoriasiform skin inflammation and express various S1P receptors. How they respond to S1P in skin inflammation remains unknown. We show that myeloid specific S1P receptor 1 (S1PR1) deletion enhances early inflammation in a mouse model of imiquimod-induced psoriasis, without altering the immune cell infiltrate. Mechanistically, myeloid S1PR1 deletion altered the formation of IL-1β, VEGF-A, and VEGF-C, and their receptors’ expression in psoriatic skin, which subsequently lead to reciprocal regulation of neoangiogenesis and neolymphangiogenesis. Experimental findings were corroborated in human clinical datasets and in knockout macrophages in vitro. Increased blood vessel but reduced lymph vessel density may explain the exacerbated inflammatory phenotype in conditional knockout mice. These findings assign a novel role to macrophage S1PR1 and provide a rationale for therapeutically targeting local S1P during skin inflammation.
A Ciliary View of the Immunological Synapse
Authors: Cassioli Chiara , Baldari Cosima T.
Published: July 2019

The primary cilium has gone from being a vestigial organelle to a crucial signaling hub of growing interest given the association between a group of human disorders, collectively known as ciliopathies, and defects in its structure or function. In recent years many ciliogenesis proteins have been observed at extraciliary sites in cells and likely perform cilium-independent functions ranging from regulation of the cytoskeleton to vesicular trafficking. Perhaps the most striking example is the non-ciliated T lymphocyte, in which components of the ciliary machinery are repurposed for the assembly and function of the immunological synapse even in the absence of a primary cilium. Furthermore, the specialization traits described at the immunological synapse are similar to those seen in the primary cilium. Here, we review common regulators and features shared by the immunological synapse and the primary cilium that document the remarkable homology between these structures.
Microstructure and Properties of M3B2-Type Boride-Based Cermet Coatings Prepared by Laser Cladding Synthesis
Authors: Hu Zhaowei , Li Wenge , Zhao Yuantao
Published: July 2019

Although Q235 steel materials are widely used in offshore engineering, the service life is severely shortened by its inferior resistance to wear and corrosion in harsh marine working environments. Boride-based cermet composites could be a good surface-protective coating to enhance surface hardness, wear resistance, and corrosion resistance. M3B2 (M: Mo, Ni, Fe, Cr) boride-based cermet coatings composed of hard ceramics of M3B2-type complex borides and an {Fe, Ni} metal matrix was fabricated on Q235 steels with mixed Mo, Cr, B, and Ni60 powders using a laser cladding synthesis technique. The influences of laser cladding parameters on the microstructure, phase composition, microhardness, and corrosion resistance of the coatings were comprehensively investigated. Results showed that the microstructures of the coatings mainly consisted of three layers, which were, from the top to bottom layer, a metal layer with fewer ceramic phases, a ceramic layer with fewer metal phases, and another metal layer with fewer ceramic phases. The ceramic phases were mainly M3B2-type borides, and the metal phases were mainly {Fe, Ni} alloys. The appearance of Fe-enriching metal phases was due to the supply of Fe elements from Q235 substrates. With squash pretreatment and without a remelting aftertreatment, ceramics uniformly dispersed in the cermet coatings, and their sizes decreased. The results of microhardness showed that the microhardness of the coating first increased and then decreased from the top layer to the bottom layer, and maximum microhardness was obtained in the layer of ceramics with less metal phases. An electrochemical corrosion test showed that the cermet coatings (jcorr = 6.35 μA/cm2) could improve the corrosion resistance of Q235 steels (j = 43.76 μA/cm2) by one order of magnitude.
Surface Morphology of Silicon Waveguide after Reactive Ion Etching (RIE)
Authors: Zheng Yu , Gao Piaopiao , Jiang Lianqiong , Kai Xiaochao , Duan Ji’an
Published: July 2019

The side wall profile roughness of the silicon waveguide prepared by electron beam lithography and reactive ion etching is extracted by using the boundary tracing method. The maximum, minimum, and average roughness values are extracted from the side wall boundary, and the changes of the side wall boundary of waveguide after electron beam exposure and reactive ion etching were compared. The roughness variation of the waveguide side wall is similar with the same length. And roughness from the bottom of the waveguide etched region is measured directly by laser confocal microscope and roughness correlation statistics are also obtained.
Ecological Ammonium Thiocyanate-Modified Geopolymeric Coating for Flame-Retarding Plywood
Authors: Wang Yachao , Zhao Jiangping
Published: July 2019

An ecological ammonium thiocyanate (NH4SCN)-modified geopolymeric coating was facilely prepared for flame-retarding plywood. The effect of NH4SCN on the flame resistance was preliminarily investigated using cone calorimeter (CC), scanning electron microscope (SEM), X-ray diffraction (XRD), and thermal gravimetry (TG). The results show that 1 wt.% NH4SCN as dopant is of paramount importance to generate a compact and continuous coating. The formation of a smooth, intact, and uniform-swelling siliceous layer during combustion facilitates enhanced fire resistance, evidenced by the increased fire performance index (FPI), reduced fire growth index (FGI), and 39.7% decreased value of peak heat release rate (pHRR), in comparison to those of the sample without NH4SCN. Because of the reducibility of O2-consuming NH4SCN, the compact shielding-layer containing carbonate and sulfate, as well as the release of NH3, the NH4SCN-modified geopolymeric coating exerts an enhancement on the flame-retardant efficiency.
Numerical Simulation of the Phase Transition Control in a Cylindrical Sample Made of Ferromagnetic Shape Memory Alloy
Authors: Rogovoy Anatoli A. , Stolbova Olga S.
Published: July 2019

The paper considers ferromagnetic alloys, which exhibit the shape memory effect during phase transition from the high-temperature cubic phase (austenite) to the low-temperature tetragonal phase (martensite) in the ferromagnetic state. In these alloys, significant macroscopic strains are generated during the direct temperature phase transition from the austenitic to the martensitic state, provided that the process proceeds under the action of the applied mechanical stresses. The critical phase transition temperatures in such alloys depend not only on the stress fields, but also on the magnetic field. By changing the magnetic field, it is possible to control the process of phase transition. In this work, within the framework of the finite deformation theory, we develop a model that allows us to describe the process of the control of the direct (austenite-martensite) and reverse (martensite-austenite) phase transitions in ferromagnetic shape memory polycrystalline materials under the action of external force, thermal, and magnetic fields with the aid of the magnetic field. In view of the fact that the magnetic field affects the material deformation, which, in turn, changes the magnetic field, we formulated and solved a coupled boundary value problem. As an example, we considered the problem of a shift of the outer surface of a long hollow cylinder made of ferromagnetic alloy. The numerical implementation of the problem was based on the finite element method using the step-by-step loading procedure. Complete recovery of the strains accumulated during the direct phase transition and reverting of the axially-displaced outer surface of the cylinder to its original position occurred both on heating of the sample to the temperatures of the reverse phase transition and at a constant temperature, when the magnetic field previously applied in the martensitic state was removed.
Dissociation Behavior of Dislocations in Ice
Authors: Hondoh Takeo
Published: July 2019

Dislocations in ice behave very differently from those in other materials due to the very low energies of stacking faults in the ice basal plane. As a result, the dislocations dissociate on the basal plane, from a perfect dislocation into two partial dislocations with equilibrium width we ranging from 20 to 500 nm, but what is the timescale to reach this dissociated state? Using physical models, we estimate this timescale by calculating two time-constants: the dissociation-completing time td and the dissociation-beginning time tb. These time constants are calculated for two Burgers vectors as a function of temperature. For perfect dislocations with Burgers vector , td is more than one month even at the melting temperature TM, and it exceeds 103 years below −50 ℃, meaning that the dissociation cannot be completed during deformation over laboratory timescales. However, in this case the beginning time tb is less than one second at TM, and it is within several tens of minutes above −50 ℃. These dislocations can glide on non-basal planes until they turn to the dissociated state during deformation, finally resulting in sessile extended dislocations of various widths approaching to the equilibrium value we. In contrast, for perfect dislocations with Burgers vector , td is less than one second above −50 ℃, resulting in glissile extended dislocations with the equilibrium width we on the basal plane. This width is sensitive to the shear stress τ exerted normal to the dislocation line, leading to extension of the intervening stacking fault across the entire crystal grain under commonly accessible stresses. Also, due to the widely dissociated state, dislocations  cannot cross-slip to non-basal planes. Such behavior of extended dislocations in ice are notable when compared to those of other materials.
Monochromatic Depolarizer Based on Liquid Crystal
Authors: Marć Paweł , Bennis Noureddine , Spadło Anna , Kalbarczyk Aleksandra , Węgłowski Rafał , Garbat Katarzyna , Jaroszewicz Leszek R.
Published: July 2019

Polarization is a very useful parameter of a light beam in many optical measurements. Improvement of holographic systems requires optical elements which need a diffused and depolarized light beam. This paper describes a simple monochromatic depolarizer based on a pure vertically aligned liquid crystal without pretilt. In this work we present an extended description of depolarizer by analyzing its electro-optic properties measured in spatial and time domains with the use of crossed polarizers and polarimetric configurations. Crossed polarizers set-up provides information on spatial and temporal changes of microscopic textures while polarimetric measurement allows to measure voltage and time dependence of degree of polarization. Three different thicknesses, i.e., 5 μm, 10 μm and 15 μm have been manufactured in order to analyze another degree of freedom for this type of depolarizer device based on a liquid crystals’ material. Consideration of the light scattering capability of the cell is reported.
Catastrophic Household Expenditure for Healthcare in Turkey: Clustering Analysis of Categorical Data
Authors: Dogan Onur , Kaya Gizem , Kaya Aycan , Beyhan Hidayet
Published: July 2019

The amount of health expenditure at the household level is one of the most basic indicators of development in countries. In many countries, health expenditure increases relative to national income. If out-of-pocket health spending is higher than the income or too high, this indicates an economical alarm that causes a lower life standard, called catastrophic health expenditure. Catastrophic expenditure may be affected by many factors such as household type, property status, smoking and drinking alcohol habits, being active in sports, and having private health insurance. The study aims to investigate households with respect to catastrophic health expenditure by the clustering method. Clustering enables one to see the main similarity and difference between the groups. The results show that there are significant and interesting differences between the five groups. C4 households earn more but spend less money on health problems by the rate of 3.10% because people who do physical exercises regularly have fewer health problems. A household with a family with one adult, landlord and three people in total (mother or father and two children) in the cluster C5 earns much money and spends large amounts for health expenses than other clusters. C1 households with elementary families with three children, and who do not pay rent although they are not landlords have the highest catastrophic health expenditure. Households in C3 have a rate of 3.83% health expenditure rate on average, which is higher than other clusters. Households in the cluster C2 make the most catastrophic health expenditure.
Design and Physical Prototyping of a Novel Braking System for a Helicopter Rotor
Authors: Booker Julian D. , Lock Richard J. , Drury David
Published: July 2019

The aim of this paper was to demonstrate the improved functionality and performance of an electromechanical brake for a helicopter main rotor, which to date has been hydraulically actuated using a disc brake and caliper arrangement. Increasingly, designers seek higher performing solutions to traditional problems through the integration of modern actuation and control strategies. This electromechanical device is required to constrain the helicopter tail rotor shaft protruding from the main rotor gearbox to allow safe taxiing and storage of the helicopter. A systematic and rigorous design methodology was used to converge on an effective solution which satisfied a very demanding specification. The design was further detailed and optimized, leading to the development of a prototype at a high technology readiness level that was tested within a bespoke rig, simulating the torque requirements found on a helicopter main rotor using the torque and position control. The design was shown to meet the required holding torque whilst providing additional functionality of continuous holding capability and meeting the challenging volumetric constraints.