bluuebunny/crossref_metadata_2025 huggingface dataset:
Resting-state Functional Connectivity After Occipital Stroke
Authors: Räty Silja , Ruuth Riikka , Silvennoinen Katri , Sabel Bernhard A. , Tatlisumak Turgut , Vanni Simo
Published: December 2021

Background

Resting-state functional magnetic resonance imaging (rsfMRI) reflects spontaneous activation of cortical networks. After stroke, these networks reorganize, both due to structural lesion and reorganization of functional connectivity (FC).

Objective

We studied FC in chronic phase occipital stroke patients with homonymous visual field defects before and after repetitive transorbital alternating current stimulation (rtACS).

Methods

This spin-off study, embedded in the randomized, sham-controlled REVIS trial, comprised 16 chronic occipital stroke patients with visual field defect and 12 healthy control subjects. The patients underwent rsfMRI at baseline, after two weeks of rtACS or sham treatment, and after two months of treatment-free follow-up, whereas the control subjects were measured once. We used a multivariate regression connectivity model to determine mutual prediction accuracy between 74 cortical regions of interest. Additionally, the model parameters were included into a graph to analyze average path length, centrality eigenvector, centrality degree, and clustering of the network. The patients and controls at baseline and the two treatment groups were compared with multilevel modeling.

Results

Before treatment, the patients and controls had similar whole-network prediction accuracy and network parameters, whereas centrality eigenvector differed in perilesional regions, indicating local modification in connectivity. In line with behavioral results, neither prediction accuracy nor any network parameter changed systematically as a result of rtACS rehabilitation compared to sham.

Conclusions

Whole-network FC showed no difference between occipital stroke patients and healthy population, congruent with the peripheral location of the visual network in relation to the high-density cortical core. rtACS treatment in the given setting did not affect FC.
Maternal Cardiovascular Disease After Pre-Eclampsia and Gestational Hypertension: A Narrative Review
Authors: Oliver-Williams Clare , Johnson Jasmine D. , Vladutiu Catherine J.
Published: December 2021

Previous literature has highlighted that women who have a pregnancy affected by gestational hypertension or preeclampsia are at higher risk of cardiovascular disease (CVD) in later life. However, CVD is a composite of multiple outcomes, including coronary heart disease, heart failure, and stroke, and the risk of both CVD and hypertensive disorders of pregnancy varies by the population studied. We conducted a narrative review of the risk of cardiovascular outcomes for women with prior gestational hypertension and pre-eclampsia. Previous literature is summarized by country and ethnicity, with a higher risk of CVD and coronary heart disease observed after gestational hypertension and a higher risk of CVD, coronary heart disease and heart failure observed after pre-eclampsia in most of the populations studied. Only one study was identified in a low- or middle-income country, and the majority of studies were conducted in white or mixed ethnicity populations. We discuss potential interventions to mitigate cardiovascular risk for these women in different settings and highlight the need for a greater understanding of the epidemiology of CVD risk after gestational hypertension and pre-eclampsia outside of high-income, white populations.
MicroRNA-17-5p, a novel endothelial cell modulator, controls vascular re-endothelialization and neointimal lesion formation
Authors: Liu Xiaopei , Chen Jing , Liu Gen , Zhang Bofang , Jin Xing , Wang Yun
Published: December 2021

Background

The functions of miR-17-5p in tumorigenesis have been explored. However, their functionalities in arterial endothelial cells (ECs) have not been investigated. Besides, the issue of vascular remodelling is barely addressed.

Objectives

The study aimed to determine the effect of overexpression or inhibition of miR-17-5p on arterial endothelial cells’ (ECs) function and vascular remodelling in vitro and the rat carotid arteries model.

Methods

Quantitative RT-PCR analysis was performed to examine the expression of miR-17-5p. Then, gain-of-function and loss-of-function approaches were employed to investigate the functional roles of miR-17-5p in cultured human coronary artery endothelial cells (HCAECs); further, TargetScan software analysis and luciferase reporter activity assay were performed to investigate the potential mechanism. Lastly, the results of the cell segment were verified in a rat carotid artery balloon injury model by Western blot analysis, measurement of the vascular cGMP level and plasma 8-iso-prostaglandin F2 (8-iso-PGF2) testing. Moreover, morphometric analysis was implemented to detect the re-endothelialization and neointimal formation in rat carotid artery after balloon injury.

Results

This study firstly found that miR-17-5p expression was upregulated in the injured vascular walls and highly expressive in ECs; overexpression of miR-17-5p inhibited HCAECs’ proliferation and migration, whereas miR-17-5p knockdown strengthened its proliferative and migratory roles, influenced inflammatory response, through regulating VEGRA and VEGFR2. It was found that miR-17-5p bind to VEGFA and VEGFR2 at the 3′UTR. Next, downregulation of miR-17-5p promotes re-endothelialization, and attenuates neointimal formation as measured by the I/M ratio (0.63±0.05 vs 1.45±0.06, antagomiR-17-5p vs. Lenti-NC, p < 0.05). In addition, the functional recovery of the endothelium was also accelerated by miR-17-5p knockdown.

Conclusion

Our study suggests that miR-17-5p is a feasible strategy for the selective modulation of endothelialization and vascular remodelling through regulating VEGFA and VEGFR2.
Different change of direction tests assess different physical ability parameters: Principal component analysis of nine change of direction tests
Authors: Kozinc Žiga , Šarabon Nejc
Published: December 2021

Change of direction (CoD) ability is critical for the success of athletes in many sports. The purpose of this study was to perform a principal component analysis using 9 CoD tests in order to reveal possible subcomponents of CoD ability, which could aid practitioners in test selection. Male and female kinesiology students (n = 76) performed all CoD tests and a 30-m sprint test in a quasi-randomized, counterbalanced order. Three components for males and two components for females were extracted from principle component analysis (variance explained = 82.3 and 71.4%, respectively). It seems that the CoD test should be subdivided into at least two components: a) “pure CoD tests” (such as 505 test, T-test and 180° turn) and maneuverability tests (such as AFL run, Illinois test and Figure of Eight test). Considering that different factors seem to underlie CoD and maneuverability, our findings have important practical implications for training design. If hopping/jumping CoD is important for a given athlete, it should also be tested separately.
A time-varying reliability-based robust design method considering overall efficiency of axial piston pump
Authors: Du Zunling , Zhang Yimin
Published: December 2021

Axial piston pumps (APPs) are the core energy conversion components in a hydraulic transmission system. Energy conversion efficiency is critically important for the performance and energy-saving of the pumps. In this paper, a time-varying reliability design method for the overall efficiency of APPs was established. The theoretical and practical instantaneous torque and flow rate of the whole APP were derived through comprehensive analysis of a single piston-slipper group. Moreover, as a case study, the developed model for the instantaneous overall efficiency was verified with a PPV103-10 pump from HYDAC. The time-variation of reliability for the pump was revealed by a fourth-order moment technique considering the randomness of working conditions and structure parameters, and the proposed reliability method was validated by Monte Carlo simulation. The effects of the mean values and variance sensitivity of random variables on the overall efficiency reliability were analyzed. Furthermore, the optimized time point and design variables were selected. The optimal structure parameters were obtained to meet the reliability requirement and the sensitivity of design variables was significantly reduced through the reliability-based robust design. The proposed method provides a theoretical basis for designers to improve the overall efficiency of APPs in the design stage.
Phylogenetic relationships and genetic variations among cinereous vultures Aegypius monachus in South Korea
Authors: Lee Mu-Yeong , Lee Seon-Mi , Kang Seung-Gu , Jeon Hye Sook , Kim Hee-Jong , Park Jin-Young , An Junghwa
Published: December 2021

Mature individuals of the cinereous vulture, Aegypius monachus, number 15,600–21,000 birds worldwide and population size of the bird has been in decline due to poisoned baits and the decreasing availability of food. Approximately 12–16% of the global population of cinereous vultures spend their winters in Korean regions that are hence important areas. In this study, the population structure and genetic diversity of the cinereous vulture in South Korea were evaluated to clarify the relationships between the Mongolian and South Korean populations with genetic diversity assessed using mitochondrial DNA (mtDNA) and microsatellite markers. In the South Korean population, two unique haplotypes (Hap8 and Hap9) were newly discovered in their mtDNA, with three polymorphic sites and low-level genetic diversity. Most of the cinereous vultures in South Korea were represented by a single haplotype, Hap8, and analyses of phylogenetic trees and the haplotype network illustrated that Hap8 was clustered in Lineage D (Mongolia). Notably, the other haplotype, Hap9, was only identified in one individual in South Korea with an unexpected clustering in Lineage C (Caucasus region, Armenia, Georgia, and Kazakhstan populations), which indicated that it might have been a vagrant bird. The microsatellite loci ( n = 21) isolated from South Korean cinereous vultures indicated comparatively lower genetic variation, with a K value of 1. These results indicate that the origin of the South Korean population is most likely from one source population of the Far Eastern cluster (Mongolian population), which was also supported by the outcome of mtDNA analysis. This study improves our understanding of the population structure and genetic diversity between the Mongolian and South Korean cinereous vulture populations.
How food supply in rubbish dumps affects the breeding success and offspring mortality of cattle Egret Bubulcus ibis?
Authors: Gherbi-Salmi Rachida , Bachir Abdelkrim Si , Ghazi Cherif , Doumandji Salah Eddine
Published: December 2021

The objective of this study was to evaluate the effect of food supply in garbage dumps on the reproductive fitness of Cattle Egret Bubulcus ibis and offspring losses. A total of 236 nests were monitored during two distinct periods of 2 years for each: 146 nests during a period without food supply in dumps (1998–1999) and 90 with food supply in dumps (2007–2008). The study was carried out in the colony of El-Kseur in the Lower Soummam Valley (northeast Algeria). For the entire study period, the mean of clutch size, average number of hatched chicks, productivity, and breeding success varied significantly between years (Kruskal–Wallis test: p < .05). Also, the average calculated losses for eggs, chicks, and total offspring vary significantly (Chi2 test: p > .0001). The clutch size and the number of hatched chicks per nest were highest during the period with food supply in garbage dump (respectively: 3.46 ± 0.86; 2.85 ± 1.11), compared to the period when cattle egrets feed in natural or agricultural habitats (3.04 ± 0.87; 2.54 ± 1.03). However, productivity and breeding success were highest during the period without food supply (respectively: 2.11 ± 1.16 fledging’s/nest; 0.70 ± 0.35) than in the period with food supply (1.14 ± 0.91; 0.35 ± 0.30). While egg losses were substantially similar between the two study periods, chick’s mortality (59.9%) and total offspring losses (36.7%) were higher during the period with food supply. The generalized linear mixed model (GLMM) analysis indicated a large negative effect of food supply in dumps on the productivity, on the chick’s losses; and a positive effect on the total offspring losses ( p < .001). Also, feed in dump garbage revealed a significant negative effect on the breeding success linear mixed model (LMM, p = .01). However, no significant effects (GLMM, p > .05) of food supply in dumps were noted on average clutch size, the mean number of hatched chicks per nest, and egg losses.
Human–nature relationships in early childhood education for sustainability: Metaphysical analysis and reshaping
Authors: Dreamson Neal , Kim Soyoung
Published: December 2021

Popular instructional approaches in Early Childhood Education for Sustainability (ECEfS) are co-construction learning, transformative learning and ecological learning. These rely on constructivism that could challenge human–nature relationships of ECEfS. In this study, we aim to discover and reshape human–nature relationships embedded in the approaches. To do so, we deconstruct the approaches through a metaphysical analysis with ontology, epistemology and axiology. As a result, we confirm that they are likely to view humans and nature as two separate entities. For sustainability, we distinguish an ontological view (‘N’ature) and an epistemological view (‘n’ature) and justify the distinction based on posthumanists’ concepts of non-humans and more-than-humans. We present a metaphysically reshaped human–nature relationship and argue that this new model enables teachers to critically review ‘human-made/observed natures’ and participate in the reconciliation between ‘N’ature and humanity. We also apply the model into a waste-recycle activity to clarify its potential practicality.
Digital manometry to measure cerebrospinal fluid pressure during lumbar puncture
Authors: Heider Robert , Kranz Peter G , Weant Erin Hope , Gray Linda , Amrhein Timothy J
Published: December 2021

Rationale and Objectives

Accurate cerebrospinal fluid (CSF) pressure measurements are critical for diagnosis and treatment of pathologic processes involving the central nervous system. Measuring opening CSF pressure using an analog device takes several minutes, which can be burdensome in a busy practice. The purpose of this study was to compare accuracy of a digital pressure measurement device with analog manometry, the reference gold standard. Secondary purpose included an assessment of possible time savings.

Materials and Methods

This study was a retrospective, cross-sectional investigation of 71 patients who underwent image-guided lumbar puncture (LP) with opening CSF pressure measurement at a single institution from June 2019 to September 2019. Exclusion criteria were examinations without complete data for both the digital and analog measurements or without recorded needle gauge. All included LPs and CSF pressures were measured with the patient in the left lateral decubitus position, legs extended. Acquired data included (1) digital and analog CSF pressures and (2) time required to measure CSF pressure.

Results

A total of 56 procedures were analyzed in 55 patients. There was no significant difference in mean CSF pressures between devices: 22.5 cm H2O digitally vs 23.1 analog ( p = .7). Use of the digital manometer resulted in a time savings of 6 min (438 s analog vs 78 s digital, p < .001).

Conclusion

Cerebrospinal fluid pressure measurements obtained with digital manometry demonstrate comparable accuracy to the reference standard of analog manometry, with an average time savings of approximately 6 min per case.
Practice Variation Research in Degenerative Lumbar Disc Surgery: A Literature Review on Design Characteristics and Outcomes
Authors: Van Munster Juliëtte J. C. M. , de Weerdt Vera , Halperin Ilan J. Y. , Zamanipoor Najafabadi Amir H. , van Benthem Peter Paul G. , Schoonman Guus G. , Moojen Wouter A. , van den Hout Wilbert B. , Atsma Femke , Peul Wilco C.
Published: December 2021

Study Design

Literature review.

Objective

To describe whether practice variation studies on surgery in patients with lumbar degenerative disc disease used adequate study methodology to identify unwarranted variation, and to inform quality improvement in clinical practice. Secondary aim was to describe whether variation changed over time.

Methods

Literature databases were searched up to May 4th, 2021. To define whether study design was appropriate to identify unwarranted variation, we extracted data on level of aggregation, study population, and case-mix correction. To define whether studies were appropriate to achieve quality improvement, data were extracted on outcomes, explanatory variables, description of scientific basis, and given recommendations. Spearman’s rho was used to determine the association between the Extreme Quotient (EQ) and year of publication.

Results

We identified 34 articles published between 1990 and 2020. Twenty-six articles (76%) defined the diagnosis. Prior surgery cases were excluded or adjusted for in 5 articles (15%). Twenty-three articles (68%) adjusted for case-mix. Variation in outcomes was analyzed in 7 articles (21%). Fourteen articles (41%) identified explanatory variables. Twenty-six articles (76%) described the evidence on effectiveness. Recommendations for clinical practice were given in 9 articles (26%). Extreme Quotients ranged between 1-fold and 15-fold variation and did not show a significant change over time (rho= −.33, P= .09).

Conclusions

Practice variation research on surgery in patients with degenerative disc disease showed important limitations to identify unwarranted variation and to achieve quality improvement by public reporting. Despite the availability of new evidence, we could not observe a significant decrease in variation over time.
Psychometric Properties of the Korean Version of the Perceived Therapeutic Efficacy Scale for Physical Activity in Adults With Type 2 Diabetes
Authors: Kim Moonsun , Kim Chun-Ja , Kim Dae Jung , Schlenk Elizabeth A.
Published: December 2021

Purpose:

The purpose of this study is to translate the Perceived Therapeutic Efficacy Scale (PTES) into Korean and investigate its validity and reliability.

Methods:

The authors conducted a cross-sectional survey using baseline data from a randomized controlled study to psychometrically validate the PTES-Korean (PTES-K) among 108 adults with type 2 diabetes from an outpatient clinic at a university-affiliated hospital in Korea. The original PTES was forward-translated and back-translated to ensure translation equivalence of the PTES-K. Structured questionnaires were used for psychometric evaluation; exploratory and confirmatory factor analysis assessed validity, and Cronbach’s alpha coefficient and intraclass correlation coefficient (ICC) were used for reliability.

Results:

The interitem correlation analyses revealed that 5 items were redundant; thus, the scale was reduced to 5 items. A 1-factor model explained 76.85% of the variance; confirmatory factor analysis showed that this model adequately fit the data. The ICC for test-retest reliability was .78; Cronbach’s alpha was .92. The PTES-K showed significant associations with the scores of diabetes self-care activities for physical activity, quality of life, and depressive symptoms. Participants with good glycemic control and regular physical activity tended to have a higher score on the PTES-K than their counterparts, demonstrating known-groups validity.

Conclusions:

The cross-cultural applicability, reliability, and validity of the PTES-K were confirmed. The PTES-K may be used in clinical settings to examine the potential role of perceived therapeutic efficacy for physical activity in enhanced glycemic control among patients with diabetes.
MicroRNA-148a-3p suppresses cell proliferation and migration of esophageal carcinoma by targeting CEP55
Authors: Lin Yong , Chen Yushan , Shen Rongqiang , Chen Dingzhu , Lin Yimin
Published: December 2021

This study evaluated microRNA-148a-3p in esophageal carcinoma cells. The prediction of bioinformatics analysis revealed that microRNA-148a-3p may target CEP55. qRT-PCR and western blot showed that CEP55 level in esophageal carcinoma cells and tissue was dramatically higher than that of normal cells and tissue, while microRNA-148a-3p was the opposite. Forced expression of microRNA-148a-3p restrained cell malignant behaviors of esophageal carcinoma, and repression of microRNA-148a-3p resulted in the converse results in terms of cell function. Dual-luciferase assay confirmed that microRNA-148a-3p targeted CEP55. CEP55 attenuated the suppressive effect of microRNA-148a-3p on proliferation and migration of esophageal carcinoma cells, demonstrating that microRNA-148a-3p regulated function of esophageal carcinoma cells via decreasing CEP55 level. Microscopy observation indicated that cell morphology was also affected by the microRNA-148a-3p/CEP55 axis. Furthermore, western blot analysis revealed that the PI3K/AKT signaling pathway could be suppressed by activating the microRNA-148a-3p/CEP55 axis. Finally, in vivo experiments confirmed the effects of microRNA-148a-3p on tumorigenesis. Thus, microRNA-148a-3p could act as a repressor in esophageal carcinoma via binding to CEP55.
cDNA-detector: detection and removal of cDNA contamination in DNA sequencing libraries
Authors: Qi Meifang , Nayar Utthara , Ludwig Leif S. , Wagle Nikhil , Rheinbay Esther
Published: December 2021

Background

Exogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that gene in next-generation sequencing libraries derived from this system. If not properly recognized and managed, this cross-contamination with exogenous signal can lead to incorrect interpretation of research results. Yet, this problem is not routinely addressed in current sequence processing pipelines.

Results

We present cDNA-detector, a computational tool to identify and remove exogenous cDNA contamination in DNA sequencing experiments. We demonstrate that cDNA-detector can identify cDNAs quickly and accurately from alignment files. A source inference step attempts to separate endogenous cDNAs (retrocopied genes) from potential cloned, exogenous cDNAs. cDNA-detector provides a mechanism to decontaminate the alignment from detected cDNAs. Simulation studies show that cDNA-detector is highly sensitive and specific, outperforming existing tools. We apply cDNA-detector to several highly-cited public databases (TCGA, ENCODE, NCBI SRA) and show that contaminant genes appear in sequencing experiments where they lead to incorrect coverage peak calls.

Conclusions

cDNA-detector is a user-friendly and accurate tool to detect and remove cDNA detection in NGS libraries. This two-step design reduces the risk of true variant removal since it allows for manual review of candidates. We find that contamination with intentionally and accidentally introduced cDNAs is an underappreciated problem even in widely-used consortium datasets, where it can lead to spurious results. Our findings highlight the importance of sensitive detection and removal of contaminant cDNA from NGS libraries before downstream analysis.
Immunological effects of convalescent plasma therapy for coronavirus: a scoping review
Authors: Esmaeili Behnaz , Esmaeili Shahnaz , Pourpak Zahra
Published: December 2021

Background

Preliminary studies revealed the safety and effectiveness of convalescent plasma (CP) therapy for patients with coronavirus. In this study, we aimed to evaluate and summarize the available evidence on CP therapy, identify the research gap regarding the immunological response to CP therapy and pave the road for future studies.

Methods

This study was conducted according to the Hilary Arksey and Lisa O’Malley framework. To find out the relevant studies, we searched PubMed, Scopus and Embase databases up to 30th May 2021. Data have been extracted according to three categories: (1) patients’ characteristics, (2) clinical and immunological responses to CP therapy and (3) pre-infusion screening of the CP samples.

Results

A total of 12,553 articles were identified. One hundred fifty-four studies met the inclusion criteria for full-text review. More than half of the included studies (112 studies, (75.6%)) concluded satisfactory outcomes and or safety of CP infusion in patients. Results of studies showed the efficacy of CP therapy in clinical improvement (101 studies), decreasing in the level of inflammatory factors (62 studies), elimination or decreasing in viral load (60 studies), and induction or increase in antibody response (37 studies). Despite these promising results, the results of the 49 studies revealed that CP therapy was ineffective in the survival of patients, clinical improvement, viral infection elimination or decrease in the inflammatory factor levels. Furthermore, the adaptive immune response was evaluated in 3 studies. Information related to the pre-infusion screening for human leukocyte antigen/human neutrophil antigen (HLA/HNA) antibodies was not reported in most of the studies. Our gap analysis revealed that the influence of the CP infusion on the adaptive immune and inflammatory responses in patients with coronavirus needs further investigation.

Conclusions

Based on the results of most included studies, CP infusion was safe and resulted in clinical improvement of patients and decreasing the viral load. The effect of the CP infusion on adaptive immune response and inflammatory cytokines in patients with coronavirus needs further investigation.
Pericytes as mediators of infiltration of macrophages in multiple sclerosis
Authors: Kaushik Deepak Kumar , Bhattacharya Anindita , Lozinski Brian Mark , Wee Yong V.
Published: December 2021

Background

Multiple sclerosis (MS) is a neurodegenerative condition of the central nervous system (CNS). It is associated with blood–brain barrier (BBB) breakdown and intravasation of leukocytes, particularly monocyte-derived macrophages, into the CNS. Pericytes are mural cells that are encased within the basement membrane of vasculature, and they contribute functionally to the neurovascular unit. These cells play an important role in maintaining BBB integrity and CNS homeostasis. However, the critical role of pericytes in mediating inflammation in MS or its models is unclear. Whether pericytes infiltrate into the CNS parenchyma in MS also needs clarification.

Methods

CNS samples from the experimental autoimmune encephalomyelitis (EAE) mouse model of MS were collected at different time points for immunohistochemical analysis of pericytes along the inflamed vasculature. These findings were validated using MS brain specimens, and further analysis of pericyte involvement in inflammation was carried out by culturing primary pericytes and macrophages. Multiplex ELISA, transmigration assay and real-time PCR were used to study the inflammatory potential of pericytes in cultures.

Results

We found that pericytes exhibit a heterogenous morphology, with notable elongation in the inflamed perivascular cuffs of EAE. This was manifested by a decrease in pericyte density but an increase in the coverage by pericytes along the vasculature. Chondroitin sulfate proteoglycans (CSPGs), a family of extracellular matrix proteins enriched within inflamed perivascular cuffs, elevated levels of pro-inflammatory chemokines/cytokines in pericytes in culture. Importantly, pericytes stimulated with CSPGs enhanced macrophage migration. We did not detect pericytes in the CNS parenchyma during EAE, and this was corroborated in MS brain samples.

Conclusions

Our data suggest that pericytes seek to restore the BBB through increased coverage, but that their exposure to CSPGs prompt their facilitation of macrophages to enter the CNS to elevate neuroinflammation in EAE and MS.
“COVID affected us all:” the birth and postnatal health experiences of resettled Syrian refugee women during COVID-19 in Canada
Authors: Stirling Cameron Emma , Ramos Howard , Aston Megan , Kuri Marwa , Jackson Lois
Published: December 2021

Background

Prior to COVID-19, postnatal resettled refugee women in Canada reported barriers to healthcare and low levels of social support, contributing to maternal health morbidities. The COVID-19 pandemic appears to be further exacerbating health inequities for marginalized populations. The experiences of resettled refugee women are not fully known.

Aim

To understand Syrian refugee women’s experiences accessing postnatal healthcare services and supports during the COVID-19 pandemic.

Methods

Semi-structured, virtual interviews were conducted with eight resettled Syrian refugee women living in Nova Scotia (Canada) who were postnatal between March and August 2020. Data analysis was informed by constructivist grounded theory.

Findings

Three themes emerged: “the impacts of COVID-19 on postnatal healthcare;” “loss of informal support;” and “grief and anxiety.” Women experienced difficult healthcare interactions, including socially and physically isolated deliveries, challenges accessing in-person interpreters, and cancelled or unavailable in-home services (e.g., public health nurse and doula visits). Increased childcare responsibilities and limited informal supports due to pandemic restrictions left women feeling overwhelmed and exhausted. Stay-at-home orders resulted in some women reporting feelings of isolation and loss, as they were unable to share in person postnatal moments with friends and family, ultimately impacting their mental wellness.

Conclusions

COVID-19 and associated public health restrictions had significant impacts on postnatal Syrian refugee women. Data presented in this study demonstrated the ways in which the pandemic environment and related restrictions amplified pre-existing barriers to care and postnatal health inequalities for resettled refugee women—particularly a lack of postnatal informal supports and systemic barriers to care.
Upright versus supine MRI: effects of body position on craniocervical CSF flow
Authors: Muccio Marco , Chu David , Minkoff Lawrence , Kulkarni Neeraj , Damadian Brianna , Damadian Raymond V. , Ge Yulin
Published: December 2021

Background

Cerebrospinal fluid (CSF) circulation between the brain and spinal canal, as part of the glymphatic system, provides homeostatic support to brain functions and waste clearance. Recently, it has been observed that CSF flow is strongly driven by cardiovascular brain pulsation, and affected by body orientation. The advancement of MRI has allowed for non-invasive examination of the CSF hydrodynamic properties. However, very few studies have addressed their relationship with body position (e.g., upright versus supine). It is important to understand how CSF hydrodynamics are altered by body position change in a single cardiac phase and how cumulative long hours staying in either upright or supine position can affect craniocervical CSF flow.

Methods

In this study, we investigate the changes in CSF flow at the craniocervical region with flow-sensitive MRI when subjects are moved from upright to supine position. 30 healthy volunteers were imaged in upright and supine positions using an upright MRI. The cranio-caudal and caudo-cranial CSF flow, velocity and stroke volume were measured at the C2 spinal level over one cardiac cycle using phase contrast MRI. Statistical analysis was performed to identify differences in CSF flow properties between the two positions.

Results

CSF stroke volume per cardiac cycle, representing CSF volume oscillating in and out of the cranium, was ~ 57.6% greater in supine (p < 0.0001), due to a ~ 83.8% increase in caudo-cranial CSF peak velocity during diastole (p < 0.0001) and extended systolic phase duration when moving from upright (0.25 ± 0.05 s) to supine (0.34 ± 0.08 s; p < 0.0001). Extrapolation to a 24 h timeframe showed significantly larger total CSF volume exchanged at C2 with 10 h spent supine versus only 5 h (p < 0.0001).

Conclusions

In summary, body position has significant effects on CSF flow in and out of the cranium, with more CSF oscillating in supine compared to upright position. Such difference was driven by an increased caudo-cranial diastolic CSF velocity and an increased systolic phase duration when moving from upright to supine position. Extrapolation to a 24 h timeframe suggests that more time spent in supine position increases total amount of CSF exchange, which may play a beneficial role in waste clearance in the brain.
Transcriptome analysis reveals the molecular mechanism of Yiqi Rougan decoction in reducing CCl4-induced liver fibrosis in rats
Authors: Xiong Yu , Hu Jinyuan , Xuan Chen , Tian Jiayu , Tan Kaiyue , Chen Zhiwei , Luo Yan , Du Xuqin , Cheng Junxiong , Zhang Lanyue , Cao Wenfu
Published: December 2021

Background

Liver fibrosis develops from various chronic liver diseases, and there is currently a lack of specific treatment strategies. Yiqi Rougan decoction (YQRG) is a traditional Chinese medicine that has shown durative effects in the treatment of liver fibrosis; however, the mechanism associated with YQRG-related improvements in liver fibrosis remains to be experimentally determined. This study evaluated the therapeutic effect of YQRG on carbon tetrachloride (CCl4)-induced liver fibrosis in rats and its molecular mechanism.

Methods

We used low-, medium-, and high-dose YQRG to treat CCl4-induced liver fibrosis in rats, followed by assessment of liver injury and fibrosis according to liver appearance, body weight, liver mass index, histopathologic examination, and serum testing. Additionally, we performed transcriptome analysis using RNA-sequencing (RNA-seq) technology, including cluster, Gene Ontology (GO), and pathway analyses, to identify differentially expressed genes (DEGs), and protein and gene expression were detected by immunofluorescence (IFC), western blot and real-time quantitative PCR.

Results

The results showed that YQRG effectively alleviated CCl4-induced liver injury and fibrosis in rats, including observations of improved liver function, decreased activity of hepatic stellate cells (HSCs), and decreased extracellular matrix (ECM) deposition. Moreover, we identified downregulated and upregulated DEGs in the model group relative to the control and YQRG-treated groups, with GO analysis revealing their enrichment in biological processes, such as endoplasmic reticulum stress (ERS), apoptosis, and autophagy. Furthermore, pathway analysis showed that YQRG treatment downregulated the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/Akt (PI3K/AKT) signalling pathways and upregulated other signalling pathways, including those related to peroxisome proliferator-activated receptors(PPAR) and AMP-activated protein kinase(AMPK), with these findings subsequently verified experimentally.

Conclusion

These findings showed that YQRG improved CCl4-induced liver fibrosis through multiple mechanisms and pathways, offering critical insight into the YQRG-related therapeutic mechanism and promoting further research into its potential application.
TorsiFlex: an automatic generator of torsional conformers. Application to the twenty proteinogenic amino acids
Authors: Ferro-Costas David , Mosquera-Lois Irea , Fernández-Ramos Antonio
Published: December 2021

In this work, we introduce , a user-friendly software written in Python 3 and designed to find all the torsional conformers of flexible acyclic molecules in an automatic fashion. For the mapping of the torsional potential energy surface, the algorithm implemented in combines two searching strategies: preconditioned and stochastic. The former is a type of systematic search based on chemical knowledge and should be carried out before the stochastic (random) search. The algorithm applies several validation tests to accelerate the exploration of the torsional space. For instance, the optimized structures are stored and this information is used to prevent revisiting these points and their surroundings in future iterations. operates with a dual-level strategy by which the initial search is carried out at an inexpensive electronic structure level of theory and the located conformers are reoptimized at a higher level. Additionally, the program takes advantage of conformational enantiomerism, when possible. As a case study, and in order to exemplify the effectiveness and capabilities of this program, we have employed to locate the conformers of the twenty proteinogenic amino acids in their neutral canonical form. has produced a number of conformers that roughly doubles the amount of the most complete work to date.

Graphical Abstract
Long-term follow-up and quality of life in patients receiving extracorporeal membrane oxygenation for pulmonary embolism and cardiogenic shock
Authors: Stadlbauer Andrea , Philipp Alois , Blecha Sebastian , Lubnow Matthias , Lunz Dirk , Li Jing , Terrazas Armando , Schmid Christof , Lange Tobias J. , Camboni Daniele
Published: December 2021

Background

Since 2019, European guidelines recommend considering extracorporeal life support as salvage strategy for the treatment of acute high-risk pulmonary embolism (PE) with circulatory collapse or cardiac arrest. However, data on long-term survival, quality of life (QoL) and cardiopulmonary function after extracorporeal membrane oxygenation (ECMO) are lacking.

Methods

One hundred and nineteen patients with acute PE and severe cardiogenic shock or in need of mechanical resuscitation (CPR) received venoarterial or venovenous ECMO from 2007 to 2020. Long-term data were obtained from survivors by phone contact and personal interviews. Follow-up included a QoL analysis using the EQ-5D-5L questionnaire, echocardiography, pulmonary function testing and cardiopulmonary exercise testing.

Results

The majority of patients (n = 80, 67%) were placed on ECMO during or after CPR with returned spontaneous circulation. Overall survival to hospital discharge was 45.4% (54/119). Nine patients died during follow-up. At a median follow-up of 54.5 months (25–73; 56 ± 38 months), 34 patients answered the QoL questionnaire. QoL differed largely and was slightly reduced compared to a German reference population (EQ5D5L index 0.7 ± 0.3 vs. 0.9 ± 0.04; p  < 0.01). 25 patients (73.5%) had no mobility limitations, 22 patients (65%) could handle their activities, while anxiety and depression were expressed by 10 patients (29.4%). Return-to-work status was 33.3% (average working hours: 36.2 ± 12.5 h/per week), 15 (45.4%) had retired from work early. 12 patients (35.3%) expressed limited exercise tolerance and dyspnea. 59% (20/34) received echocardiography and pulmonary function testing, 50% (17/34) cardiopulmonary exercise testing. No relevant impairment of right ventricular function and an only slightly reduced mean peak oxygen uptake (76.3% predicted) were noted.

Conclusions

Survivors from severe intractable PE in cardiogenic shock or even under CPR with ECMO seem to recover well with acceptable QoL and only minor cardiopulmonary limitations in the long term. To underline these results, further research with larger study cohorts must be obtained.
Marketing authorization of COVID-19 vaccines across UK, EU, and the US: fact-checking and the implications for future research
Authors: Abbas Nasir , Babar Zaheer-Ud-Din
Published: December 2021

While having access to safe and efficient vaccines is essential for eradicating the COVID-19 pandemic, gaining marketing authorisation is a critical step in enabling and speeding this process. On December 2, 2020, the United Kingdom became the first country to approve the first COVID-19 vaccine. This commentary aims to provide a quick overview of the UK’s COVID-19 vaccine authorization process and compare it to that of the EU and the US. While the UK, EU, and US expedited the COVID-19 vaccine approval process, regulatory authorities did not appear to cut corners in their approval of the Pfizer COVID-19 vaccine, as evidenced by their decisions to switch emergency use authorization to full authorization in the US and to renew conditional/temporary use authorization in the EU and UK, respectively. There is an opportunity to conduct a thorough investigation into and comparison of the filed dossiers, as well as the robustness of the evaluation process for the approval of COVID-19 vaccines.
Urgent care study of the LumiraDx SARS-CoV-2 Ag Test for rapid diagnosis of COVID-19
Authors: Gresh Jared , Kisner Harold , DuChateau Brian
Published: December 2021

Background

Testing individuals suspected of severe acute respiratory syndrome–like coronavirus 2 (SARS-CoV-2) infection is essential to reduce the spread of disease. The purpose of this retrospective study was to determine the false negativity rate of the LumiraDx SARS-CoV-2 Ag Test when utilized for testing individuals suspected of SARS-CoV-2 infection.

Methods

Concurrent swab samples were collected from patients suspected of SARS-CoV-2 infection by their healthcare provider within two different urgent care centers located in Easton, MA, USA and East Bridgewater, MA, USA. One swab was tested using the LumiraDx SARS-CoV-2 Ag Test. Negative results in patients considered at moderate to high risk of SARS-CoV-2 infection were confirmed at a regional reference laboratory by polymerase chain reaction (PCR) using the additional swab sample. The data included in this study was collected retrospectively as an analysis of routine clinical practice.

Results

From October 19, 2020 to January 3, 2021, a total of 2241 tests were performed using the LumiraDx SARS-CoV-2 Ag Test, with 549 (24.5%) testing positive and 1692 (75.5%) testing negative. A subset (800) of the samples rendering a negative LumiraDx SARS-CoV-2 Ag Test was also tested using a PCR-based test for SARS-CoV-2. Of this subset, 770 (96.3%) tested negative, and 30 (3.8%) tested positive. Negative results obtained with the LumiraDx SARS-CoV-2 Ag test demonstrated 96.3% agreement with PCR-based tests (CI 95%, 94.7–97.4%). A cycle threshold (CT) was available for 17 of the 30 specimens that yielded discordant results, with an average CT value of 31.2, an SD of 3.0, and a range of 25.2–36.3. CT was > 30.0 in 11/17 specimens (64.7%).

Conclusions

This study demonstrates that the LumiraDx SARS-CoV-2 Ag Test had a low false-negative rate of 3.8% when used in a community-based setting.
Notching is less, if femoral component sagittal positioning is planned perpendicular to distal femur anterior cortex axis, in navigated TKA
Authors: Kanna Raj , Ravichandran Chandramohan , Shetty Gautam M.
Published: December 2021

Purpose

In navigated TKA, the risk of notching is high if femoral component sagittal positioning is planned perpendicular to the sagittal mechanical axis of femur (SMX). We intended to determine if, by opting to place the femoral component perpendicular to distal femur anterior cortex axis (DCX), notching can be reduced in navigated TKA.

Methods

We studied 171 patients who underwent simultaneous bilateral computer-assisted TKA. Femoral component sagittal positioning was planned perpendicular to SMX in one knee (Femur Anterior Bowing Registration Disabled, i.e. FBRD group) and perpendicular to DCX in the opposite knee (Femur Anterior Bowing Registration Enabled, i.e. FBRE group). Incidence and depth of notching were recorded in both groups. For FBRE knees, distal anterior cortex angle (DCA), which is the angle between SMX and DCX, was calculated by the computer.

Results

Incidence and mean depth of notching was less (p = 0.0007 and 0.009) in FBRE versus FBRD group, i.e. 7% versus 19.9% and 0.98 mm versus 1.53 mm, respectively. Notching was very high (61.8%) in FBRD limbs when the anterior bowing was severe (DCA > 3°) in the contralateral (FBRE) limbs.

Conclusion

Notching was less when femoral component sagittal positioning was planned perpendicular to DCX, in navigated TKA.

Level of evidence

Therapeutic level II.
Ni-Catalyzed Dearomative Cycloaddition of Alkynes to 10π Aromatic Benzothiophenes: Elucidation of Reaction Mechanism
Authors: Takahashi Toshifumi , Kurahashi Takuya , Matsubara Seijiro
Published: December 2021

The Ni(0)-catalyzed cycloaddition of alkynes to stable 10π-electron aromatic benzothiophenes involves the insertion of the former into the C–S bond of the latter to form metastable 12π-electron non-aromatic benzothiepines featuring a seven-membered ring, with the reactivity of benzothiophenes strongly influenced by their substituents (e.g., MeO and F). Herein, the mechanism of the above dearomative cycloaddition is elucidated by computational and experimental methods. The promotional effects of benzothiophene substituents (MeO and F) on the catalytic reaction are rationalized by density functional theory computations. Moreover, computations demonstrate the superiority of Ni catalysts over Pd ones. The theoretical findings are confirmed by spectroscopic (operando X-ray absorption fine structure) analysis, and the combined results are used to design an alternative reaction-promoting substituent, the effect of which is then experimentally validated. A deep mechanistic understanding of this unprecedented dearomative cycloaddition is important for the design of a new range of reactions involving dearomatization.
Synthesis and Polymerization of Acid-degradable Rotaxane Using Boc Protecting Group
Authors: Nomura Tsugumi , Onimura Kenjiro , Yamabuki Kazuhiro
Published: December 2021

An acid-degradable rotaxane consisting of a crown ether/ammonium salt complex was synthesized using a stopper compound bearing a tert-butyloxycarbonyl protecting group (Boc group). The unstable inclusion complex was stabilized by introducing a bulky Boc group at its end. The deprotection reaction via addition of trifluoroacetic acid resulted in the decomposition of rotaxane, as demonstrated by 1H nuclear magnetic resonance (NMR) and gel permeation chromatography measurement. The polymerization of rotaxane by metathesis reaction was also attempted.
First record of the genus Restiosporium (Websdaneaceae, Ustilaginales) outside Australasia: documenting R. spathacei in Thailand
Authors:   Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Denchev Teodor T. , Denchev Cvetomir M. ,   Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences
Published: December 2021

The genus Restiosporium consists of 21 species on hosts in the Restionaceae. It was known only from Australia and New Zealand. Restiosporium is reported here for the first time outside Australasia, based on a specimen of R. spathacei on Dapsilanthus disjunctus from Thailand. A description and illustrations are provided for this smut fungus. Dapsilanthus disjunctus is a new host plant for Restiosporium spathacei.