bluuebunny/crossref_metadata_2025 huggingface dataset:
College Students’ Opinions About Coping Strategies for Mental Health Problems, Suicide Ideation, and Self-Harm During COVID-19
Authors: Klonoff-Cohen Hillary
Published: July 2022

Background

Mental health problems have emerged as a significant health complication in United States colleges during COVID-19, and as a result, they have been extensively investigated in the United States and internationally. In contrast, research on coping among the college population during the pandemic is scant. Hence, this study investigated coping strategies proposed by undergraduate students attending a Midwestern university.

Objectives

The purpose of this preliminary study was to obtain college students’ feedback/opinions about coping strategies for mental health problems, suicide ideation, and self-harm during COVID-19.

Methods

In December 2021, one-hundred and four undergraduate students (ages 18–22 years) completed an online survey on coping strategies using Qualtrics. Major topics included: (1) Types of coping strategies/styles expressed by students for general mental health problems, (2) Types of coping strategies for suicide ideation and self-harm behaviors, (3) Preferred platforms for receiving coping therapy, and (4) Reasons for accepting or refusing parent involvement with mental health problems.

Results

The most beneficial coping strategies for mental health were ranked by college students as follows: (1) a skills training development program (30%), (2) meditation (19%), and (3) mindfulness exercises (15%), and physical education (11%). The respondents’ best coping strategies for preventing self-harm and suicide ideation/behaviors during COVID-19 were ranked as: (1) improving support from friends (32%), (2) building self-esteem (29%), and (3) addressing anger, depression, stress, and loneliness (25%). Finally, a total of 50% of participants felt that parents should be involved in college student interventions. Students stated that the most important type of support that they received from their parents were: (1) emotional support (31%), (2) direction and/or assistance with solutions (27%), and problem-solving (16%).

Conclusion

This study identified potential avenues which could be implemented into action during future outbreaks. Specifically, employing interventions that: (i) train undergraduate students to employ more effective skills training coping strategies or practicing mindfulness or meditation; (ii) integrate mental health, suicide, and self-harm prevention into the curriculum; (iii) offer more in-person campus services targeted toward the psychological and emotional effects of a pandemic, and (iv) involve support persons (e.g., family) in students’ lives to enhance their well-being during and after COVID-19.
MRI-Visible Perivascular Spaces Associated With Cognitive Impairment in Military Veterans With Traumatic Brain Injury Mediated by CSF P-Tau
Authors: Wang Ming-Liang , Yang Dian-Xu , Sun Zheng , Li Wen-Bin , Zou Qiao-Qiao , Li Peng-Yang , Wu Xue , Li Yue-Hua
Published: July 2022

Objective

To investigate the association of MRI-visible perivascular spaces (PVS) with cognitive impairment in military veterans with traumatic brain injury (TBI), and whether cerebrospinal fluid (CSF) p-tau and Aβ mediate this effect.

Materials and Methods

We included 55 Vietnam War veterans with a history of TBI and 52 non-TBI Vietnam War veterans from the Department of Defense Alzheimer’s Disease Neuroimaging Initiative (ADNI) database. All the subjects had brain MRI, CSF p-tau, Aβ, and neuropsychological examinations. MRI-visible PVS number and grade were rated on MRI in the centrum semiovale (CSO-PVS) and basal ganglia (BG-PVS). Multiple linear regression was performed to assess the association between MRI-visible PVS and cognitive impairment and the interaction effect of TBI. Additionally, mediation effect of CSF biomarkers on the relationship between MRI-visible PVS and cognitive impairment was explored in TBI group.

Results

Compared with military control, TBI group had higher CSO-PVS number (p = 0.001), CSF p-tau (p = 0.022) and poorer performance in verbal memory (p = 0.022). High CSO-PVS number was associated with poor verbal memory in TBI group (β = -0.039, 95% CI −0.062, −0.016), but not in military control group (β = 0.019, 95% CI −0.004, 0.043) (p-interaction = 0.003). Further mediation analysis revealed that CSF p-tau had a significant indirect effect (β = −0.009, 95% CI: −0.022 −0.001, p = 0.001) and mediated 18.75% effect for the relationship between CSO-PVS and verbal memory in TBI group.

Conclusion

MRI-visible CSO-PVS was more common in Vietnam War veterans with a history of TBI and was associated with poor verbal memory, mediated partially by CSF p-tau.
COVID-19 Inpatient Deaths and Brought-in-Dead Cases in Malaysia
Authors: Lim Poh Ying , Md Said Salmiah , Kadir Shahar Hayati , Azman Ahmad Zaid Fattah , Mokhtar Siti Aisah , Mahmud Aidalina
Published: July 2022

Coronavirus disease 2019 (COVID-19) deaths can occur in hospitals or otherwise. In Malaysia, COVID-19 deaths occurring outside of the hospital and subsequently brought to the hospital are known as brought-in-dead (BID) cases. To date, the characteristics of BID COVID-19 cases in Malaysia are not clear. The objectives of this study are 2-fold: to explore the characteristics of 29,155 mortality cases in Malaysia and determine the factors associated with the high probability of BID, using the multilevel logistic regression model. Data on COVID-19 mortality cases from the entire country between March 17, 2020 and November 3, 2021 were retrieved from a national open data source. Of the 29,155 COVID-19 mortality cases, 5,903 (20.2%) were BID. A higher probability of BID (p < 0.05) was seen among individuals aged between 18 and 59 years, non-Malaysians, had no comorbidities, did not receive COVID-19 vaccination, and the interval between the date of death and diagnosis. A high prevalence of BID is an alarming public health issue, as this may signal health system failure at one or several levels and, hence, need urgent attention from relevant stakeholders. Based on the findings of this study, increasing the intensity of the vaccination campaign, addressing any issues faced by noncitizens about to COVID-19 management in- and out-of-hospital, increasing the awareness of signs and symptoms of worsening COVID-19 and, hence, the significance of self-monitoring, and determining the potential gaps in the health system may contribute to their increased risk of deaths.
Athletic Identity and Shoulder Overuse Injury in Competitive Adolescent Tennis Players: The Smash Cohort Study
Authors: Johansson Fred , Tranaeus Ulrika , Asker Martin , Skillgate Eva , Johansson Fredrik
Published: July 2022

Objectives

Our primary aim was to determine if athletic identity is prospectively associated with shoulder overuse injuries. Secondly, we aimed to determine if athletic identity is prospectively associated with playing through pain and to describe how athletic identity relates to sex, age, playing level, weekly training load, and match volume.

Methods

A cohort of 269 adolescent tennis players were followed over a period of 52 weeks. Cox regression was used to estimate the hazard rate ratio (HRR) of first-time shoulder overuse injury associated with every 10-unit increase on the Athletic Identity Measurement Scale (AIMS).

Results

The adjusted HRR of shoulder overuse injury was 0.89 (95% CI: 0.36–2.20) and the odds ratio of playing through pain was 2.41 (95% CI: 0.74–8.96) for every 10 unit increase on AIMS. The level of athletic identity was higher among players at the national level than among players at the regional level and was weakly correlated to weekly hours of tennis matches, tennis training, and fitness training.

Conclusions

Our results indicate that higher levels of athletic identity may be associated with a lower incidence of shoulder overuse injuries, and potentially with playing through pain, although these results are inconclusive due to wide confidence intervals.
Evaluation of Availability, Prices, and Affordability of Selected Essential Medicines in Balochistan, Pakistan
Authors: Bibi Murad , Haq Noman Ul , Kareem Abdul , Ullah Habib , Baloch Nizam , Rehman Gulalai , Nasim Aqeel
Published: July 2022

Objectives: The study aimed to evaluate the availability, prices, and affordability of selected essential medicines in Balochistan, Pakistan.

Methods: Cross-sectional research was conducted in several cities of Balochistan, Pakistan, using the World Health Organization/Health Action International methodology to assess the availability and cost of 50 originator brand (OB) and lowest priced generic (LPG) drugs. The medicine costs were compared to international reference prices (IRPs) to calculate the median price ratio. The daily wage of the lowest paid unskilled government employee was used to determine affordability.

Results: The mean availability was low for OBs (9.8%) and fairly high (49.4%) for LPGs. The OBs and LPGs’ mean availability in the private sector were fairly high, 51.8% and 42.6%, respectively. It was surprising to see that Balochistan’s public sector has only 24.3% of the National Essential Medicine List when the medicines on this list are supposed to be adequately available.

Conclusion: The standard treatment cost with OBs is steep, exceeding the minimum daily wage. Treatment with LPG medications seems affordable. Furthermore, essential LPG medicines are economical when used solely for medication therapy.
Praca zdalna jako wyzwanie dla organizacji i funkcjonowania urzędów administracji publicznej w warunkach pandemii COVID-19
Authors: Kuligowski Rafał , Lipska-Sondecka Agnieszka
Published: July 2022

The COVID-19 pandemic has become one of the greatest global challenges of recent years. It forced the governments of many countries to introduce new and unique legal instruments, whose main objective was to prevent, counteract and combat the infectious disease caused by a novel coronavirus. One of these instruments was the possibility to limit the functioning of public administration offices. This accelerated the design and implementation of solutions which, on the one hand, ensured the safety and health protection of employees of public offices and, on the other hand, allowed the continuation of public tasks. The subject of the article is the analysis of Polish legal solutions concerning the possibility of remote work in connection with the outbreak of the COVID-19 pandemic. The aim of the study is to show that the coronavirus pandemic forced the Polish legislator to introduce new instruments into the legal order regarding the organisation and functioning of the state administrative apparatus. One of them was the possibility of remote work, i.e. work that is carried out away from the place of its permanent performance.
Storage of the Early Ripe Almonds under Modified Atmosphere to Preserve Kernel Qualitative and Sensory Traits
Authors: Massantini Riccardo , Cristofori Valerio , Frangipane Maria Teresa
Published: July 2022

Almonds are often used both in confectionery products and for fresh consumption. Thus, to enhance the use of early ripe or unripe fresh almonds, it is important to maintain the organoleptic and qualitative traits of the product for a period of time as long as possible. The objective of the research was to study different types of almonds storage, not artificially dried, under a modified atmosphere to maintain quality in almond kernels mainly destoned to table consumption. The storage of samples was in a modified atmosphere in 100 ± 1 kPa CO2, 100 ± 1 kPa N2 or air and at +4 °C and +10 °C, respectively for 12 days. Some analytical parameters and sensory analysis were explored. Test results showed that the modified atmosphere of N2 at +4 °C was the most suitable for keeping the chemical, physical and sensorial attributes of fresh almond kernels, maintaining their quality intact for up to almost two weeks of storage.
Comparison of the Effects of Chemical Topping Agent Sprayed by a UAV and a Boom Sprayer on Cotton Growth
Authors: Dou Zechen , Fang Zhihao , Han Xiaoqiang , Liu Yapeng , Duan Li , Zeeshan Muhammad , Arshad Muhammad
Published: July 2022

In order to improve the spraying efficiency of cotton chemical topping agents, a field experiment was conducted to compare the effects of 250 g·L−1 mepiquat chloride aqueous solutions sprayed by a T30 unmanned aerial vehicle (UAV) and a boom sprayer. The cotton agronomic parameters, pesticide utilization rate, yield, and fiber quality were determined. The results showed that the pesticide utilization rates of the T30 UAV and the boom sprayer were 48.71% and 30.37%, respectively. After 10, 20, 30, and 40 days of T30 UAV spraying, the plant height inhibition rates of the two varieties of cotton were 12.97%, 13.78%, 20.91%, and 26.14% for Xinluzao 52 and 8.64%, 13.37%, 14.72%, and 18.03% for Huiyuan 720. After spraying with a boom sprayer, the plant height inhibition rates were 7.94%, 11.13%, 18.23%, and 23.69% for Xinluzao 52 and 6.09%, 9.98%, 11.78%, and 15.14% for Huiyuan 720 after 10, 20, 30, and 40 days of spraying. The T30 UAV spraying chemical topping agent was shown to have a significant beneficial effect on the utilization rate of the pesticides, as well as the agronomic properties, yield, and the fiber quality of the cotton.
Wildlife Crime: Issues and Promising Solutions
Authors: Pires Stephen F. , Olah George
Published: July 2022

The poaching of wildlife for profit, pleasure, subsistence, or as a result of human–animal conflict has decimated wildlife populations—particularly those of at-risk species [...]
Evidence-Based Recommendations for Herd Health Management of Porcine Post-Weaning Diarrhea
Authors: Eriksen Esben Østergaard , Pedersen Ken Steen , Larsen Inge , Nielsen Jens Peter
Published: July 2022

Aided by their advising veterinarians, pig producers need to make difficult decisions regarding herd health management strategies. For instance, the preventive use of antimicrobials and medicinal zinc oxide must be substituted with more sustainable preventive approaches to porcine post-weaning diarrhea. Veterinarians and pig producers may find assistance in knowledge based on evidence in this regard; however, the overwhelming scientific literature is not always readily available. The overall aim of this paper is to suggest herd health management decision-support tools that can aid veterinary-assisted decision making in the control of porcine post-weaning diarrhea at a tactical level. The first objective was to validate the need for a herd health management concept, including two decision-support tools. The second objective was to develop evidence-based recommendations that can aid veterinary-assisted decision-making for the herd health management of post-weaning diarrhea. The first objective was investigated by a questionnaire-based study among veterinary pig practitioners in Denmark. For the second objective, we conducted a scientific summary based on scientific review papers identified through a systematic search in three databases. From the papers, we synthesized and extracted 79 specific recommendations. In this paper, we report comprehensive evidence-based recommendations for the herd health management of post-weaning diarrhea.
A Rare Case of Endophthalmitis with Rhizobium radiobacter, Soon after a Resolved Keratitis: Case Report
Authors: Balasoiu Andrei Theodor , Zlatian Ovidiu Mircea , Ghenea Alice Elena , Davidescu Livia , Lungu Alina , Golli Andreea Loredana , Udriștoiu Anca-Loredana , Balasoiu Maria
Published: July 2022

Background: Rhizobium (Agrobacterium) species are plant aerobic bacteria, which in some cases can produce endophthalmitis in humans after corneal trauma. Case presentation: A 42-year-old female patient presented in the Emergency Department of the Emergency County Hospital of Craiova, Romania, reporting pain, epiphora, and blurry vision in her right eye for about five days. This initial infectious keratitis episode was successfully resolved, but after 20 days she presented again after trauma with a leaf with corneal abscess. In the conjunctival secretion, R. radiobacter was identified. Despite antibiotherapy, the patient’s state did not improve, and ultimately the eye was eviscerated. Methods: A search was performed in the ProQuest, PubMed, and ScienceDirect databases for the terms Agrobacterium, Rhizobium, radiobacter, and eye. We eliminated non-human studies, editorials and commentaries, and non-relevant content, and excluded the duplicates. Results: In total, 138 studies were initially obtained, and then we selected 26 studies for retrieval. After the selection process, we ended up including 17 studies in our analysis. Most studies reported R. radiobacter endophthalmitis after ocular surgical procedures or outdoor activities that involve exposure to soil. Conclusion: R. radiobacter is a rare cause of endophthalmitis after eye trauma that generally responds well to usual antibiotherapy, but occasionally can evolve to severe, leading to the loss of the eye.
Imaging of Lipid Peroxidation-Associated Chemiluminescence in Plants: Spectral Features, Regulation and Origin of the Signal in Leaves and Roots
Authors: Havaux Michel , Ksas Brigitte
Published: July 2022

Plants, like most living organisms, spontaneously emit photons of visible light. This ultraweak endogenous chemiluminescence is linked to the oxidative metabolism, with lipid peroxidation constituting a major source of photons in plants. We imaged this signal using a very sensitive cooled CCD camera and analysed its spectral characteristics using bandpass interference filters. In vitro oxidation of lipids induced luminescence throughout the visible spectrum (450–850 nm). However, luminescence in the red spectral domain (>640 nm) occurred first, then declined in parallel with the appearance of the emission in the blue-green (<600 nm). This temporal separation suggests that the chemical species emitting in the blue-green are secondary products, possibly deriving from the red light-emitting species. This conversion did not seem to occur in planta because spontaneous chemiluminescence from plant tissues (leaves, roots) occurred only in the red/far-red light domain (>640 nm), peaking at 700–750 nm. The spectrum of plant chemiluminescence was independent of chlorophyll. The in vivo signal was modulated by cellular detoxification mechanisms and by changes in the concentration of singlet oxygen in the tissues, although the singlet oxygen luminescence bands did not appear as major bands in the spectra. Our results indicate that the intensity of endogenous chemiluminescence from plant tissues is determined by the balance between the formation of luminescent species through secondary reactions involving lipid peroxide-derived intermediates, including singlet oxygen, and their elimination by metabolizing processes. The kinetic aspects of plant chemiluminescence must be taken into account when using the signal as an oxidative stress marker.
Design of Intelligent Management Platform for Industry–Education Cooperation of Vocational Education by Data Mining
Authors: Wu Min , Hao Xinxin , Lv Yang , Hu Zihan
Published: July 2022

Data are playing an increasingly important role in the development of industry–education cooperation strategies in vocational education and training. The objective of this study was to promote the comprehensive progress of an industry–education cooperation system and improve the effect of the application of big data technology in this system. First, we designed of a big data technology application in an intelligent management platform system for industry–education cooperation. Second, we analyzed the synthetical design of the system. Finally, we optimized and designed a support vector machine (SVM) data mining (DM) algorithm model based on big data, and evaluated the model. The results revealed that the designed algorithm model provides outstanding advantages compared with similar algorithm models. In general, the highest average computation time of the designed SVM algorithm model is about 95 ms. The overall average calculation time linearly decreases around 200 iterations and tends to be stable, and the lowest overall average computation time is about 20 ms. In the DM process, the highest accuracy rate of the model is about 97%, and the lowest is about 92%. The DM accuracy rate is always stable as the number of iterations of the model continues to increase. The designed model slowly increases the occupancy rate of the system in the process of increasing computing time. At about 60 min, the system occupancy rate of the model tends to be stable, and the highest is maintained at about 23%. This study not only provides technical support for the optimization of DM algorithms with big data technology, but also contributes to the integrated development of industry–education cooperation systems.
Combating Label Noise in Image Data Using MultiNET Flexible Confident Learning
Authors: Popowicz Adam , Radlak Krystian , Lasota Slawomir , Szczepankiewicz Karolina , Szczepankiewicz Michal
Published: July 2022

Deep neural networks (DNNs) have been used successfully for many image classification problems. One of the most important factors that determines the final efficiency of a DNN is the correct construction of the training set. Erroneously labeled training images can degrade the final accuracy and additionally lead to unpredictable model behavior, reducing reliability. In this paper, we propose MultiNET, a novel method for the automatic detection of noisy labels within image datasets. MultiNET is an adaptation of the current state-of-the-art confident learning method. In contrast to the original, our method aggregates the outputs of multiple DNNs and allows for the adjustment of detection sensitivity. We conduct an exhaustive evaluation, incorporating four widely used datasets (CIFAR10, CIFAR100, MNIST, and GTSRB), eight state-of-the-art DNN architectures, and a variety of noise scenarios. Our results demonstrate that MultiNET significantly outperforms the confident learning method.
Process Parameter Optimization for 3D Printed Investment Casting Wax Pattern and Its Post-Processing Technique
Authors: Mukhtarkhanov Muslim , Shehab Essam , Ali Md. Hazrat
Published: July 2022

This research paper aims to improve the quality of 3D printed parts made of the wax filament by implementing the Taguchi orthogonal array process optimization method. The manufactured parts can be used as cost-effective investment casting patterns. With the Taguchi method, it was concluded that the nozzle temperature has the most effect on the dimensional accuracy of printed parts. In addition, thermal, mechanical, and rheological characterization were performed on the wax filament, revealing several important findings. For instance, the rheological studies identified the low viscosity of melted wax at printing temperatures. This resulted in the rough surface of the printed parts. To improve the surface roughness, a post-processing procedure was implemented using a white spirit as a surface smoothing agent.
Balanced Meshless Method for Numerical Simulation of Pollutant Transport by Shallow Water Flow over Irregular Bed: Application in the Strait of Gibraltar
Authors: Al Nuwairan Muneerah , Chaabelasri Elmiloud
Published: July 2022

This paper focuses on the implementation of an accurate meshless scheme for the simulation of the advection–diffusion of non-active pollutant in a two-dimensional depth-averaged flow. The depth-averaged flow model includes the shallow water system and the pollutant propagation described by the advection–diffusion equation with diffusion tensor. The mathematical model was implemented by using a meshless method based on local radial basis functions. This method was used to numerically evaluate the spatial derivatives and complemented with the second-order Runge–Kutta method for the time evolution. To remove the non-physical oscillations, which appear at the discontinuity, a filter based on a hyperviscosity operator was applied. To investigate the effectiveness and accuracy of the proposed scheme, a number of tests are presented, including the dam break problem, and the pure transport of a pollutant in a long channel. Finally, a hypothetical example of a pollutant transport in the Strait of Gibraltar is modeled. The results obtained are compared both with analytical solutions and with simulation results obtained by a finite volume method based on the Roe-MUSCL scheme. The main advantages of the proposed method are: (i) the simplicity of implementation, (ii) the ability to handle calculations of slowly varying flows or concentrations, as well as rapidly varying flows containing shocks or discontinuities, and (iii) the ability to satisfy the C-property and guarantee positive values of both water level and pollutant concentration.
Simultaneous Detection of Foodborne Pathogens Using a Real-Time PCR Triplex High-Resolution Melt Assay
Authors: Reese Kashiya R. , Elkins Kelly M.
Published: July 2022

Foodborne pathogens pose risks to populations all over the world. Pathogens can be used as bioterrorism agents, causing an outbreak that affects many individuals through the consumption of a commonly affected food or beverage. A PCR assay can be used to identify pathogens through their unique melting points using a high-resolution melt assay. Assays can be used to detect the bacteria individually or from a mixture using species-specific primers. An assay was developed to detect and identify three pathogens that routinely cause multistate foodborne outbreaks, as documented by the U.S. Centers for Disease Control and Prevention, Campylobacter jejuni (C. jejuni), Escherichia coli (E. coli), and Salmonella enterica (S. enterica), in single bacterium assays and a multiplex. The primers were targeted to specific and unique gene sequences of each pathogen, including cadF, yedN, and hilA, respectively. Each pathogen was identified by its unique melting temperature in single assays: 78.10 ± 0.58 °C for C. jejuni, 81.96 ± 0.42 °C for E. coli, and 87.55 ± 0.37 °C for S. enterica. The multiplex successfully detected and identified all three of the pathogens with the distinctly separated melt peaks. The PCR high-resolution melt assay also proved to be specific, reproducible, fast, and sensitive in experiments.
Influence of Meteorological Factors and Chemical Processes on the Explosive Growth of PM2.5 in Shanghai, China
Authors: Sun Wenwen , Huo Juntao , Fu Qingyan , Zhang Yuxin , Lin Xiangde
Published: July 2022

In order to explore the mechanism of haze formation, the meteorological effect and chemical reaction process of the explosive growth (EG) of PM2.5 were studied. In this study, the level of PM2.5, water-soluble inorganic ions, carbonaceous aerosols, gaseous precursors, and meteorological factors were analyzed in Shanghai in 2018. The EG event is defined by a net increase of PM2.5 mass concentration greater than or equal to 100 μg m−3 within 3, 6, or 9 h. The results showed that the annual average PM2.5 concentration in Shanghai in 2018 was 43.2 μg m−3, and secondary inorganic aerosols and organic matter (OM) accounted for 55.8% and 20.1% of PM2.5, respectively. The increase and decrease in the contributions of sulfate, nitrate, ammonium (SNA), and elemental carbon (EC) to PM2.5 from clean days to EG, respectively, indicated a strong, secondary transformation during EG. Three EG episodes (Ep) were studied in detail, and the PM2.5 concentration in Ep3 was highest (135.7 μg m−3), followed by Ep2 (129.6 μg m−3), and Ep1 (82.3 μg m−3). The EG was driven by stagnant conditions and chemical reactions (heterogeneous and gas-phase oxidation reactions). This study improves our understanding of the mechanism of haze pollution and provides a scientific basis for air pollution control in Shanghai.
Characterizing Long Island’s Extreme Precipitation and Its Relationship to Tropical Cyclones
Authors: Reed Austin T. , Stansfield Alyssa M. , Reed Kevin A.
Published: July 2022

Since extreme precipitation impacts society on small scales (i.e., a few kilometers and smaller, it is worthwhile to explore extreme precipitation trends in localized regions, such as Long Island (LI), New York. Its coastal location makes it vulnerable to various extreme events, such as tropical cyclones (TCs). This work aimed to quantify the extreme precipitation events on LI that are caused by TCs, as well as the percentage of TCs passing close to LI that cause extreme precipitation events. Both gauge-based and satellite-based precipitation datasets of varying resolutions (DAYMET, IMERG, and CPC) were used to understand the impact of dataset selection. Results are shown for the common time period of 2001–2020, as well as the full time periods of each dataset. DAYMET shows the highest percentage of extreme precipitation events linked to TCs for 2001–2020 (a maximum of 7.2%) and the highest number of TCs that caused extreme precipitation events (36.5%), with IMERG showing similar results. For the full and common time periods, the highest percentage of extreme precipitation events caused by TCs was found in eastern LI. TC-related extreme precipitation averaged over LI varied year to year, and amounts were dependent on the resolution of the observational dataset, but most datasets showed an increasing trend in the last 19 years that is larger than the trend in mean precipitation. Current infrastructure in the region is likely inadequately prepared for future impacts from TC-related extreme precipitation events in such a population-dense region.
Goats as Valuable Animal Model to Test the Targeted Glutamate Supplementation upon Antral Follicle Number, Ovulation Rate, and LH-Pulsatility
Authors: Luna-García Luis A. , Meza-Herrera César A. , Pérez-Marín Carlos C. , Corona Rebeca , Luna-Orozco Juan R. , Véliz-Deras Francisco G. , Delgado-Gonzalez Ramón , Rodriguez-Venegas Rafael , Rosales-Nieto Cesar A. , Bustamante-Andrade Jorge A. , Gutierrez-Guzman Ulises N.
Published: July 2022

The potential effect of intravenous administration of glutamate on the ovarian activity and the LH secretion pattern, considering the anestrous yearling goat as an animal model, were assessed. In late April, yearling goats (n = 20) were randomly assigned to either (1) Glutamate supplemented (GLUT; n = 10, Live Weight (LW) = 29.6 ± 1.02 kg, Body Condition (BCS) = 3.4 ± 0.2 units; i.v. supplemented with 7 mg GLUT kg−1 LW) or (2) Non-supplemented (CONT; n = 10; LW = 29.2 ± 1.07 kg, BCS = 3.5 ± 0.2 units; i.v. saline). The oats were estrus-synchronized; blood sampling (6 h × 15 min) was carried out for LH quantification. Response variables included pulsatility (PULSE), time to first pulse (TTFP), amplitude (AMPL), nadir (NAD), and area under the curve (AUC) of LH. Ovaries were ultra-sonographically scanned to assess ovulation rate (OR), number of antral follicles (AF), and total ovarian activity (TOA = OR + AF). LH-PULSE was quantified with the Munro algorithm; significant treatment x time interactions were evaluated across time. The variables LW and BCS did not differ (p > 0.05) between the experimental groups. Nevertheless, OR (1.77 vs. 0.87 ± 0.20 units), TOA (4.11 vs. 1.87 ± 0.47 units) and LH-PULSE (5.0 vs. 2.2 pulses 6 h-1) favored (p < 0.05) to the GLUT group. Our results reveal that targeted glutamate supplementation, the main central nervous system neurotransmitter, arose as an interesting strategy to enhance the hypothalamic–hypophyseal–ovarian response considering the anestrous-yearling goat as an animal model, with thought-provoking while promising translational applications.
Approaches in Gene Coexpression Analysis in Eukaryotes
Authors: Zogopoulos Vasileios L. , Saxami Georgia , Malatras Apostolos , Papadopoulos Konstantinos , Tsotra Ioanna , Iconomidou Vassiliki A. , Michalopoulos Ioannis
Published: July 2022

Gene coexpression analysis constitutes a widely used practice for gene partner identification and gene function prediction, consisting of many intricate procedures. The analysis begins with the collection of primary transcriptomic data and their preprocessing, continues with the calculation of the similarity between genes based on their expression values in the selected sample dataset and results in the construction and visualisation of a gene coexpression network (GCN) and its evaluation using biological term enrichment analysis. As gene coexpression analysis has been studied extensively, we present most parts of the methodology in a clear manner and the reasoning behind the selection of some of the techniques. In this review, we offer a comprehensive and comprehensible account of the steps required for performing a complete gene coexpression analysis in eukaryotic organisms. We comment on the use of RNA-Seq vs. microarrays, as well as the best practices for GCN construction. Furthermore, we recount the most popular webtools and standalone applications performing gene coexpression analysis, with details on their methods, features and outputs.
Diffusion of Vanadium Ions in Artificial Saliva and Its Elimination from the Oral Cavity by Pharmacological Compounds Present in Mouthwashes
Authors: Fangaia Sónia I. G. , Cabral Ana M. T. D. P. V. , Nicolau Pedro M. G. , Guerra Fernando A. D. R. A. , Rodrigo M. Melia , Ribeiro Ana C. F. , Valente Artur J. M. , Esteso Miguel A.
Published: July 2022

In this study, diffusion coefficients of ammonium vanadate at tracer concentrations in artificial saliva with and without sodium fluoride, at different pH values, were measured using an experimental model based on the Taylor dispersion technique. Ternary mutual diffusion coefficients (D11, D22, D12, and D21) for four aqueous systems {NH4VO3 (component 1) + β-cyclodextrin (β-CD) (component 2),} {NH4VO3 (component 1) + β-cyclodextrin (HP-β-CD) (component 2)}, {NH4VO3 (component 1) + sodium dodecyl sulphate (SDS) (component 2)} and {NH4VO3 (component 1) + sodium hyaluronate (NaHy) (component 2)} at 25.00 °C were also measured by using the same technique. These data showed that diffusion of ammonium vanadate was strongly affected in all aqueous media studied. Furthermore, a significant coupled diffusion of this salt and β-CD was observed through the non-zero values of the cross-diffusion coefficients, D12, allowing us to conclude that there is a strong interaction between these two components. This finding is very promising considering the removal, from the oral cavity, of vanadium resulting from tribocorrosion of Ti-6Al-4V prosthetic devices.
Advances in MUC1-Mediated Breast Cancer Immunotherapy
Authors: Li Zhifeng , Yang Dazhuang , Guo Ting , Lin Mei
Published: July 2022

Breast cancer (BRCA) is the leading cause of death from malignant tumors among women. Fortunately, however, immunotherapy has recently become a prospective BRCA treatment with encouraging achievements and mild safety profiles. Since the overexpression and aberrant glycosylation of MUC1 (human mucin) are closely associated with BRCA, it has become an ideal target for BRCA immunotherapies. In this review, the structure and function of MUC1 are briefly introduced, and the main research achievements in different kinds of MUC1-mediated BRCA immunotherapy are highlighted, from the laboratory to the clinic. Afterward, the future directions of MUC1-mediated BRCA immunotherapy are predicted, addressing, for example, urgent issues in regard to how efficient immunotherapeutic strategies can be generated.
NMOSD—Diagnostic Dilemmas Leading towards Final Diagnosis
Authors: Szewczyk Anna K. , Papuć Ewa , Mitosek-Szewczyk Krystyna , Woś Michał , Rejdak Konrad
Published: July 2022

(1) Background: The emergence of white matter lesions in the central nervous system (CNS) can lead to diagnostic dilemmas. They are a common radiological symptom and their patterns may overlap CNS or systemic diseases and provoke underdiagnosis or misdiagnosis. The aim of the study was to assess factors influencing the underdiagnosis of neuromyelitis optica spectrum disorder (NMOSD) as well as to estimate NMOSD epidemiology in Lubelskie voivodeship, Poland. (2) Methods: This retrospective study included 1112 patients, who were made a tentative or an established diagnosis of acute or subacute onset of neurological deficits. The evaluation was based on medical history, neurological examination, laboratory and radiographic results and fulfilment of diagnosis criteria. (3) Results: Up to 1.62 percent of patients diagnosed with white matter lesions and up to 2.2% of the patients previously diagnosed with MS may suffer from NMOSD. The duration of delayed diagnosis is longer for males, despite the earlier age of onset. Seropositive cases for antibodies against aquaporin-4 have worse prognosis for degree of disability. (4) Conclusions: Underdiagnosis or misdiagnosis in NMOSD still remains a problem in clinical practice and has important implications for patients. The incorrect diagnosis is caused by atypical presentation or NMOSD-mimics; however, covariates such as gender, onset and diagnosis age may also have an influence.
Brain Tissue Oxygenation-Guided Therapy and Outcome in Traumatic Brain Injury: A Single-Center Matched Cohort Study
Authors: Barrit Sami , Al Barajraji Mejdeddine , El Hadweh Salim , Dewitte Olivier , Torcida Nathan , Andre Joachim , Taccone Fabio Silvio , Schuind Sophie , Gouvêa Bogossian Elisa
Published: July 2022

Brain tissue oxygenation (PbtO2)-guided therapy can improve the neurological outcome of traumatic brain injury (TBI) patients. With several Phase-III ongoing studies, most of the existing evidence is based on before-after cohort studies and a phase-II randomized trial. The aim of this study was to assess the effectiveness of PbtO2-guided therapy in a single-center cohort. We performed a retrospective analysis of consecutive severe TBI patients admitted to our center who received either intracranial pressure (ICP) guided therapy (from January 2012 to February 2016) or ICP/PbtO2-guided therapy (February 2017 to December 2019). A genetic matching was performed based on covariates including demographics, comorbidities, and severity scores on admission. Intracranial hypertension (IH) was defined as ICP > 20 mmHg for at least 5 min. Brain hypoxia (BH) was defined as PbtO2 < 20 mmHg for at least 10 min. IH and BH were targeted by specific interventions. Mann–Whitney U and Fisher’s exact tests were used to assess differences between groups. A total of 35 patients were matched in both groups: significant differences in the occurrence of IH (ICP 85.7% vs. ICP/PbtO2 45.7%, p < 0.01), ICU length of stay [6 (3–13) vs. 16 (9–25) days, p < 0.01] and Glasgow Coma Scale at ICU discharge [10 (5–14) vs. 13 (11–15), p = 0.036] were found. No significant differences in ICU mortality and Glasgow Outcome Scales at 3 months were observed. This study suggests that the role of ICP/PbtO2-guided therapy should await further confirmation in well-conducted large phase III studies.
Defining Oligometastatic Disease in the New Era of PSMA-PET Imaging for Primary Staging of Prostate Cancer
Authors: Galgano Samuel J. , McDonald Andrew M. , West Janelle T. , Rais-Bahrami Soroush
Published: July 2022

Oligometastatic prostate cancer has traditionally been defined in the literature as a limited number of metastatic lesions (either to soft tissue or bone), typically based on findings seen on CT, MRI, and skeletal scintigraphy. Although definitions have varied among research studies, many important clinical trials have documented effective treatments and prognostication in patients with oligometastatic prostate cancer. In current clinical practice, prostate-specific membrane antigen (PSMA)-PET/CT is increasingly utilized for the initial staging of high-risk patients and, in many cases, detecting metastases that would have otherwise been undetected with conventional staging imaging. Thus, patients with presumed localized and/or oligometastatic prostate cancer undergo stage migration based on more novel molecular imaging. As a result, it is challenging to apply the data from the era before widespread PET utilization to current clinical practice and to relate current trials using PSMA-PET/CT for disease detection to older studies using conventional staging imaging alone. This manuscript aims to review the definition of oligometastatic prostate cancer, summarize important studies utilizing both PSMA-PET/CT and conventional anatomic imaging, discuss the concept of stage migration, and discuss current problems and challenges with the current definition of oligometastatic disease.
Genetic Control of Kinetochore-Driven Microtubule Growth in Drosophila Mitosis
Authors: Popova Julia V. , Pavlova Gera A. , Razuvaeva Alyona V. , Yarinich Lyubov A. , Andreyeva Evgeniya N. , Anders Alina F. , Galimova Yuliya A. , Renda Fioranna , Somma Maria Patrizia , Pindyurin Alexey V. , Gatti Maurizio
Published: July 2022

Centrosome-containing cells assemble their spindles exploiting three main classes of microtubules (MTs): MTs nucleated by the centrosomes, MTs generated near the chromosomes/kinetochores, and MTs nucleated within the spindle by the augmin-dependent pathway. Mammalian and Drosophila cells lacking the centrosomes generate MTs at kinetochores and eventually form functional bipolar spindles. However, the mechanisms underlying kinetochore-driven MT formation are poorly understood. One of the ways to elucidate these mechanisms is the analysis of spindle reassembly following MT depolymerization. Here, we used an RNA interference (RNAi)-based reverse genetics approach to dissect the process of kinetochore-driven MT regrowth (KDMTR) after colcemid-induced MT depolymerization. This MT depolymerization procedure allows a clear assessment of KDMTR, as colcemid disrupts centrosome-driven MT regrowth but not KDMTR. We examined KDMTR in normal Drosophila S2 cells and in S2 cells subjected to RNAi against conserved genes involved in mitotic spindle assembly: mast/orbit/chb (CLASP1), mei-38 (TPX2), mars (HURP), dgt6 (HAUS6), Eb1 (MAPRE1/EB1), Patronin (CAMSAP2), asp (ASPM), and Klp10A (KIF2A). RNAi-mediated depletion of Mast/Orbit, Mei-38, Mars, Dgt6, and Eb1 caused a significant delay in KDMTR, while loss of Patronin had a milder negative effect on this process. In contrast, Asp or Klp10A deficiency increased the rate of KDMTR. These results coupled with the analysis of GFP-tagged proteins (Mast/Orbit, Mei-38, Mars, Eb1, Patronin, and Asp) localization during KDMTR suggested a model for kinetochore-dependent spindle reassembly. We propose that kinetochores capture the plus ends of MTs nucleated in their vicinity and that these MTs elongate at kinetochores through the action of Mast/Orbit. The Asp protein binds the MT minus ends since the beginning of KDMTR, preventing excessive and disorganized MT regrowth. Mei-38, Mars, Dgt6, Eb1, and Patronin positively regulate polymerization, bundling, and stabilization of regrowing MTs until a bipolar spindle is reformed.
The Expression and Prognostic Significance of VEGF and CXCR4 in Gastric Cancer: Correlation with Angiogenesis, Lymphangiogenesis and Progression
Authors: Kruszyna Łukasz , Murawa Dawid , Jagodziński Paweł Piotr , Oszkinis Grzegorz , Krasiński Zbigniew
Published: July 2022

The cellular response to hypoxia includes the expression of hypoxia-inducible factor-1 (HIF-1) and its target genes: vascular endothelial growth factor (VEGF) and CXC chemokine receptor 4 (CXCR4). The aim of this study was to investigate the expression and prognostic significance of VEGF and CXCR4, which are responsible for angiogenesis and progression in gastric cancer. Twenty-eight gastric cancer patients were analyzed. The mRNA expression was examined in primary tumors and corresponding normal gastric mucosa by RT-PCR. The protein level was examined by immunohistochemistry staining. The high expression of VEGF and CXCR4 was found in 71.0 and 64.0% of tumors, respectively. The mean levels of VEGF and CXCR4 were upregulated in primary tumors compared to normal mucosa (p = 0.0007, p = 0.0052). A correlation between VEGF expression and tumor invasion (p = 0.0216) and stage (p = 0.0181) was found. CXCR4 expression correlated with lymph node metastases (p = 0.0237) and stage (p = 0.0054). The VEGF expression correlated with microvessel density (MVD) (p = 0.0491). The overall 3-year survival rate was 46.4% and correlated negatively with high CXCR4 mRNA expression (p = 0.0089). VEGF and CXCR4 play an important role in tumor progression. Their overexpression correlates with a bad prognosis and may improve high-risk patient selection, and these patients may obtain additional survival benefits if treated more aggressively.
Psychological Factors Related to Impotence as a Sexual Dysfunction in Young Men: A Literature Scan for Noteworthy Research Frameworks
Authors: Ciaccio Valentina , Di Giacomo Dina
Published: July 2022

Despite the increasing interest in clinical practice in sexual dysfunction (SD) and the related relevance of psychological outcomes for young people, this area has been neglected in scientific scenarios. This study aims to draw on recent scientific findings and propose future research perspectives on the risk factors, diagnostic issues, and therapies that address SD for the under-40 age category, with particular attention paid to various psychological aspects. A literature search was conducted on studies published between March 2011 and March 2021. Anxiety, depression, and relational conflicts can both cause and affect sexual dysfunction. Psychological interventions have also proved to be important to organic causes; however, no review has found either sex education programs or multidisciplinary therapies. A multidisciplinary approach involving medical doctors and psychologists (trained in psychosexology) would improve not only the recognition of disorders through organic and psychogenic symptoms, but also treatment effectiveness.
After-Machining Surface Layer of Composite Coatings Obtained by Thermal Spraying
Authors: Starosta Robert , Dyl Tomasz
Published: July 2022

The surface of machine elements is changed to improve their mechanical properties. Ceramic-metal composite coatings are mainly used to increase the durability of machine parts. Therefore, new materials are constantly searched for application in the future development of metal and ceramic composites. The nickel and iron elements are usually used as a base and Al2O3 as tough particles. The welding technology for applying alloy and composite coatings is widely used. The common technology used to apply composite coatings is thermal spraying. It is a simple technology and very useful for the production or regeneration of machines. The surface layer is very rough and porous after applying the coating by thermal spraying. In order to obtain the proper surface quality of alloy and composite coatings, after-machining should be used. Many parts of machines strive to achieve an accurate surface. Therefore, a certain roughness is often required. The arithmetic mean of the ordinates of the surface roughness profile was (Ra = 0.16–1.25 μm). The article presents the influence of finishing on the roughness of the surface layer of the coating. After plastic working and machining, the roughness of the surface layer of the coating was reduced. However, it can be seen that, after plastic working, there is a strengthening of the surface layer of the coating and most of the pores were closed.