bluuebunny/crossref_metadata_2025 huggingface dataset:
Eradication of Bovine Mastitis Caused by the Pathogenic Microalga Prototheca bovis on a Dairy Cattle Farm: A Case Report
Authors: Beinhauerova Monika , Moravkova Monika , Seydlova Ruzena , Crhanova Magdalena
Published: September 2023

The achlorophyllous unicellular microalga of the genus Prototheca, a causative agent of bovine mammary gland infection, is receiving increasing attention in the field of veterinary medicine. Mastitis caused by these algae leads to significant economic losses for farmers worldwide and represents a source of threat to raw milk quality and dairy food-chain safety. This case report describes an outbreak of bovine mastitis in dairy cattle caused by Prototheca bovis and the on-farm practices that resulted in the recovery of the affected herd and elimination of the presence of Prototheca spp. in the farm environment. Effective management strategies that played a crucial role in protothecosis eradication included regular screening, timely identification, strict separation of Prototheca-positive cows, a change of housing regime associated with the utilisation of straw mattresses and removal of deep bedding and, finally, the introduction of intermediate disinfection of teat cups with peracetic acid to prevent the spread of infection to other healthy individuals. The eradication process lasted approximately three years and required the removal of 139 cows from the farm. The corrective and preventive measures described in this case report provide guidance to farmers on how to successfully deal with protothecal mastitis on farms.
Emerging Tick-Borne Dabie bandavirus: Virology, Epidemiology, and Prevention
Authors: Kim Eun-Ha , Park Su-Jin
Published: September 2023

Severe Fever with Thrombocytopenia Syndrome (SFTS), caused by Dabie bandavirus (SFTSV), is an emerging infectious disease first identified in China. Since its discovery, infections have spread throughout East Asian countries primarily through tick bites but also via transmission between animals and humans. The expanding range of ticks, the primary vectors for SFTSV, combined with migration patterns of tick-carrying birds, sets the stage for the global spread of this virus. SFTSV rapidly evolves due to continuous mutation and reassortment; currently, no approved vaccines or antiviral drugs are available. Thus, the threat this virus poses to global health is unmistakable. This review consolidates the most recent research on SFTSV, including its molecular characteristics, transmission pathways through ticks and other animals, as well as the progress in antiviral drug and vaccine development, encompassing animal models and clinical trials.
The Biotechnological Application of Bacteriophages: What to Do and Where to Go in the Middle of the Post-Antibiotic Era
Authors: Jo Su Jin , Kwon Jun , Kim Sang Guen , Lee Seung-Jun
Published: September 2023

Amid the escalating challenges of antibiotic resistance, bacterial infections have emerged as a global threat. Bacteriophages (phages), viral entities capable of selectively infecting bacteria, are gaining momentum as promising alternatives to traditional antibiotics. Their distinctive attributes, including host specificity, inherent self-amplification, and potential synergy with antibiotics, render them compelling candidates. Phage engineering, a burgeoning discipline, involves the strategic modification of bacteriophages to enhance their therapeutic potential and broaden their applications. The integration of CRISPR-Cas systems facilitates precise genetic modifications, enabling phages to serve as carriers of functional genes/proteins, thereby enhancing diagnostics, drug delivery, and therapy. Phage engineering holds promise in transforming precision medicine, addressing antibiotic resistance, and advancing diverse applications. Emphasizing the profound therapeutic potential of phages, this review underscores their pivotal role in combatting bacterial diseases and highlights their significance in the post-antibiotic era.
Intravenous versus Partial Oral Antibiotic Therapy in the Treatment of Uncomplicated Bloodstream Infection Due to Streptococcus Species
Authors: Broermann Lynn E. , Al-Hasan Majdi N. , Withers Sarah , Benbow Kristina L. , Ramsey Taylor , McTavish Meghan , Winders Hana R.
Published: September 2023

This retrospective cohort study examines effectiveness of partial oral antibiotic regimens in uncomplicated bloodstream infections (BSIs) due to Streptococcus species compared to standard intravenous therapy. Adult patients with uncomplicated streptococcal BSIs from April 2016 to June 2020 in seven hospitals in South Carolina, USA, were evaluated. Multivariate Cox proportional hazards regression was used to examine the time to treatment failure within 90 days of a BSI after adjustment for the propensity to receive partial oral therapy. Multivariate linear regression was used to examine the hospital length of stay (HLOS). Among the 222 patients included, 99 received standard intravenous antibiotics and 123 received partial oral therapy. Of the standard intravenous therapy group, 46/99 (46.5%) required outpatient parenteral antibiotic therapy (OPAT). There was no difference in the risk of treatment failure between partial oral and standard intravenous therapy (hazards ratio 0.53, 95% CI 0.18, 1.60; p = 0.25). Partial oral therapy was independently associated with a shorter HLOS after adjustments for the propensity to receive partial oral therapy and other potential confounders (−2.23 days, 95% CI −3.53, −0.94; p < 0.001). Transitioning patients to oral antibiotics may be a reasonable strategy in the management of uncomplicated streptococcal BSIs. Partial oral therapy does not seem to have a higher risk of treatment failure and may spare patients from prolonged hospitalizations and OPAT complications.
Acinetobacter baumannii IC2 and IC5 Isolates with Co-Existing blaOXA-143-like and blaOXA-72 and Exhibiting Strong Biofilm Formation in a Mexican Hospital
Authors: Moreno-Manjón Julia , Castillo-Ramírez Santiago , Jolley Keith A. , Maiden Martin C. J. , Gayosso-Vázquez Catalina , Fernández-Vázquez José Luis , Mateo-Estrada Valeria , Giono-Cerezo Silvia , Alcántar-Curiel María Dolores
Published: September 2023

Acinetobacter baumannii is an opportunistic pathogen responsible for healthcare-associated infections (HAIs) and outbreaks. Antimicrobial resistance mechanisms and virulence factors allow it to survive and spread in the hospital environment. However, the molecular mechanisms of these traits and their association with international clones are frequently unknown in low- and middle-income countries. Here, we analyze the phenotype and genotype of seventy-six HAIs and outbreak-causing A. baumannii isolates from a Mexican hospital over ten years, with special attention to the carbapenem resistome and biofilm formation. The isolates belonged to the global international clone (IC) 2 and the Latin America endemic IC5 and were predominantly extensively drug-resistant (XDR). Oxacillinases were identified as a common source of carbapenem resistance. We noted the presence of the blaOXA-143-like family (not previously described in Mexico), the blaOXA-72 and the blaOXA-398 found in both ICs. A low prevalence of efflux pump overexpression activity associated with carbapenem resistance was observed. Finally, strong biofilm formation was found, and significant biofilm-related genes were identified, including bfmRS, csuA/BABCDE, pgaABCD and ompA. This study provides a comprehensive profile of the carbapenem resistome of A. baumannii isolates belonging to the same pulse type, along with their significant biofilm formation capacity. Furthermore, it contributes to a better understanding of their role in the recurrence of infection and the endemicity of these isolates in a Mexican hospital.
Rafting on the Evidence for Lipid Raft-like Domains as Hubs Triggering Environmental Toxicants’ Cellular Effects
Authors: Marques-da-Silva Dorinda , Lagoa Ricardo
Published: September 2023

The plasma membrane lipid rafts are cholesterol- and sphingolipid-enriched domains that allow regularly distributed, sub-micro-sized structures englobing proteins to compartmentalize cellular processes. These membrane domains can be highly heterogeneous and dynamic, functioning as signal transduction platforms that amplify the local concentrations and signaling of individual components. Moreover, they participate in cell signaling routes that are known to be important targets of environmental toxicants affecting cell redox status and calcium homeostasis, immune regulation, and hormonal functions. In this work, the evidence that plasma membrane raft-like domains operate as hubs for toxicants’ cellular actions is discussed, and suggestions for future research are provided. Several studies address the insertion of pesticides and other organic pollutants into membranes, their accumulation in lipid rafts, or lipid rafts’ disruption by polychlorinated biphenyls (PCBs), benzo[a]pyrene (B[a]P), and even metals/metalloids. In hepatocytes, macrophages, or neurons, B[a]P, airborne particulate matter, and other toxicants caused rafts’ protein and lipid remodeling, oxidative changes, or amyloidogenesis. Different studies investigated the role of the invaginated lipid rafts present in endothelial cells in mediating the vascular inflammatory effects of PCBs. Furthermore, in vitro and in vivo data strongly implicate raft-localized NADPH oxidases, the aryl hydrocarbon receptor, caveolin-1, and protein kinases in the toxic mechanisms of occupational and environmental chemicals.
Effect of Vitamin D on the Proliferation and Barrier of Atrophic Vaginal Epithelial Cells
Authors: Li Dandan , Zhang Tao , Yang He , Yang Wenlan , Zhang Chi , Gao Guolan
Published: September 2023

Atrophic vaginitis is very common in postmenopausal women due to declining estrogen levels. Vitamin D plays an important role in promoting epithelial cell proliferation, migration and adhesion. We established a rat model of ovariectomy (OVX) induced atrophic vaginitis with the aim of investigating the effects of Vitamin D supplementation on the vaginal epithelial barrier. The results showed that ovariectomised rats had significantly higher vaginal pH, reduced Lactobacillus, significantly lower uterine and vaginal weights, and lower vaginal epithelial PCNA, occludin, and E-cadherin mRNA expression compared with sham-operated rats. Vitamin D supplementation could reduce the vaginal pH, promote the proliferation and keratinization of vaginal epithelial cells, enhance the expression of PCNA mRNA in vaginal tissues, and improve the vaginal and uterine atrophy. Vitamin D can also increase the expression of E-cadherin and occludin proteins in vaginal tissues, maintain the integrity of the vaginal epithelium, increase the number of Lactobacillus, and reduce pathogenic bacterial infections. In vitro experiments demonstrated that 1,25(OH)2D3 could promote the proliferation and migration of VK2/E6E7 vaginal epithelial cells and increase the expression of E-cadherin protein. In conclusion, we demonstrated that Vitamin D can regulate the expression of vaginal epithelial tight junction proteins, promotes cell proliferation, and improves vaginal atrophy due to estrogen deficiency.
Impact of Lactation Stage on the Metabolite Composition of Bovine Milk
Authors: Connolly Claire , Yin Xiaofei , Brennan Lorraine
Published: September 2023

Bovine milk is a nutrient-dense food and a major component of the human diet. Therefore, understanding the factors that impact its composition is of great importance. Applications of metabolomics provide in-depth analysis of the metabolite composition of milk. The objective of this research was to examine the impact of lactation stage on bovine milk metabolite levels. Metabolomic analysis of bovine milk powder samples across lactation (N = 18) was performed using nuclear magnetic resonance (1H-NMR) spectroscopy and liquid chromatography–tandem mass spectrometry (LC-MS/MS). Forty-one metabolites were identified and quantified in the 1H-NMR spectra. Statistical analysis revealed that 17 metabolites were significantly different across lactation stages (FDR < 0.05), of which the majority had higher levels in early lactation. In total, 491 metabolites were measured using LC-MS/MS, of which 269 had significantly different levels across lactation (FDR < 0.05). Compound classes significantly affected by lactation stage included phosphatidylcholines (59%) and triglycerides (64%), of which 100% of phosphatidylcholines and 61% of triglycerides increased from early lactation onwards. Our study demonstrates significant differences in metabolites across the stages of lactation, with early-lactation milk having a distinct metabolomic profile. More research is warranted to further explore these compositional differences to inform animal feeding practice.
Quantum Transport in Large-Scale Patterned Nitrogen-Doped Graphene
Authors: Lorentzen Aleksander Bach , Bouatou Mehdi , Chacon Cyril , Dappe Yannick J. , Lagoute Jérôme , Brandbyge Mads
Published: September 2023

It has recently been demonstrated how the nitrogen dopant concentration in graphene can be controlled spatially on the nano-meter scale using a molecular mask. This technique may be used to create ballistic electron optics-like structures of high/low doping regions; for example, to focus electron beams, harnessing the quantum wave nature of the electronic propagation. Here, we employ large-scale Greens function transport calculations based on a tight-binding approach. We first benchmark different tight-binding models of nitrogen in graphene with parameters based on density functional theory (DFT) and the virtual crystal approximation (VCA). Then, we study theoretically how the random distribution within the masked regions and the discreteness of the nitrogen scattering centers impact the transport behavior of sharp n−p and n−n′ interfaces formed by different, realistic nitrogen concentrations. We investigate how constrictions for the current can be realized by patterned high/low doping regions with experimentally feasible nitrogen concentrations. The constrictions can guide the electronic current, while the quantized conductance is significantly washed out due to the nitrogen scattering. The implications for device design is that a p−n junction with nitrogen corrugation should still be viable for current focusing. Furthermore, a guiding channel with less nitrogen in the conducting canal preserves more features of quantized conductance and, therefore, its low-noise regime.
Dietary Approaches from Moms, Farms, and Nature to Overcome Chronic Diseases and the Pharmacracy
Authors: Dietert Rodney R.
Published: September 2023

Chronic diseases, previously called noncommunicable diseases, are the leading cause of global death and were recently estimated by the World Health Organization to account for 74% of all deaths [...]
Usefulness of Longitudinal Strain to Assess Cancer Therapy-Related Cardiac Dysfunction and Immune Checkpoint Inhibitor-Induced Myocarditis
Authors: Tamura Yudai , Tamura Yuichi
Published: September 2023

Longitudinal strain (LS) measured by echocardiography has been reported to be useful not only for the diagnosis and risk stratification of various cardiac diseases, but also in cardio-oncology. Most previous studies have been conducted on patients undergoing treatment with anthracyclines and human epidermal growth factor receptor 2-targeted therapies. Existing guidelines recommend that global LS (GLS) should be measured before and after the administration of cancer drugs. This recommendation is based on many reports showing that a decline in GLS is indicative of early or mild cancer therapy-related cardiac dysfunction. The main purpose of this article is to provide insight into the importance of LS in patients undergoing cancer treatment and highlight the role of LS evaluation in patients undergoing immune checkpoint inhibitor (ICI) treatment, which is being used with increasing frequency. Among cancer drug therapies, immune checkpoint inhibitors (ICIs) have an important place in cancer treatment and are used for the treatment of many types of cancer. Although the efficacy of ICIs in cancer treatment has been reported, immune-related adverse events (irAEs) have also been reported. Among these irAEs, cardiovascular complications, although rare, are recognized as important adverse events that may result in ICI treatment discontinuation. Myocarditis is one severe adverse event associated with ICIs, and it is important to standardize diagnostic and therapeutic approaches to it. Several studies have reported a relationship between LS and cardiac complications associated with ICIs which may contribute to the early diagnosis of ICI-induced cardiac complications.
Interaction of ALK Inhibitors with Polyspecific Organic Cation Transporters and the Impact of Substrate-Dependent Inhibition on the Prediction of Drug–Drug Interactions
Authors: Tsang Yik Pui , López Quiñones Antonio Jesús , Vieira Letícia Salvador , Wang Joanne
Published: September 2023

Small molecules targeting aberrant anaplastic lymphoma kinase (ALK) are active against ALK-positive non-small-cell lung cancers and neuroblastoma. Several targeted tyrosine kinase inhibitors (TKIs) have been shown to interact with polyspecific organic cation transporters (pOCTs), raising concerns about potential drug–drug interactions (DDIs). The purpose of this study was to assess the interaction of ALK inhibitors with pOCTs and the impact of substrate-dependent inhibition on the prediction of DDIs. Inhibition assays were conducted in transporter-overexpressing cells using meta-iodobenzylguanidine (mIBG), metformin, or 1-methyl-4-phenylpyridinium (MPP+) as the substrate. The half-maximal inhibitory concentrations (IC50) of brigatinib and crizotinib for the substrates tested were used to predict their potential for in vivo transporter mediated DDIs. Here, we show that the inhibition potencies of brigatinib and crizotinib on pOCTs are isoform- and substrate-dependent. Human OCT3 (hOCT3) and multidrug and toxin extrusion protein 1 (hMATE1) were highly sensitive to inhibition by brigatinib and crizotinib for all three tested substrates. Apart from hMATE1, substrate-dependent inhibition was observed for all other transporters with varying degrees of dependency; hOCT1 inhibition showed the greatest substrate dependency, with differences in IC50 values of up to 22-fold across the tested substrates, followed by hOCT2 and hMATE2-K, with differences in IC50 values of up to 16- and 12-fold, respectively. Conversely, hOCT3 inhibition only showed a moderate substrate dependency (IC50 variance < 4.8). Among the substrates used, metformin was consistently shown to be the most sensitive substrate, followed by mIBG and MPP+. Pre-incubation of ALK inhibitors had little impact on their potencies toward hOCT2 and hMATE1. Our results underscore the complexity of the interactions between substrates and the inhibitors of pOCTs and have important implications for the clinical use of ALK inhibitors and their DDI predictions.
Intersection and Considerations for Patient-Centered Care, Patient Experience, and Medication Experience in Pharmacogenomics
Authors: Murry Logan T. , Hillman Lisa A. , Allen Josiah D. , Bishop Jeffrey R.
Published: September 2023

As healthcare continues to embrace the concept of person- and patient-centered care, pharmacogenomics, patient experience, and medication experience will continue to play an increasingly important role in care delivery. This review highlights the intersection between these concepts and provides considerations for patient-centered medication and pharmacogenomic experiences. Elements at the patient, provider, and system level can be considered in the discussion, supporting the use of pharmacogenomics, with components of the patient and medication experience contributing to the mitigation of barriers surrounding patient use and the valuation of pharmacogenomic testing.
High-Responsivity Planar Photodetector Based on Methylammonium Lead Bromide Perovskite Thin Film
Authors: Mai Pavlo , Houel Julien , Dreveton Nathan , Mahler Benoit , Gassenq Alban
Published: September 2023

We have fabricated planar interdigitated photodetectors exhibiting high responsivity. These detectors are based on thin layers of methylammonium lead bromide (MAPbBr3) at 90 nm thickness. MAPbBr3 thin films were first characterized on glass (borosilicate) substrates using absorption and photoluminescence measurements showing a high absorption edge at 521 nm and strong emission at 530 nm, as expected. MAPbBr3 thin films were then deposited on top of interdigitated electrodes, hence producing planar photodetectors with responsivity up to 0.4 A/W. Such higher performances were attributed to the interdigitated design, low crack density (0.05 µm−2), and lower resistivity (20 MΩ.cm) compared to MAPbBr3 single crystal. Therefore, this work highlights MAPbBr3 thin films as very promising for photodetection applications.
Gyrotropic Crystals as a Basis for Creation of Helical Polychromatic Singular Beams
Authors: Egorov Yuriy , Rubass Alexander
Published: September 2023

In this work, studies are carried out in the field of optical singular beams that have passed through gyrotropic crystals. We have experimentally shown that singular beams with a helical intensity distribution are formed when passing through a system of two gyrotropic crystals with opposite values of the gyration coefficient. It is shown that the system is capable of generating optical vortices with a double topological charge in one of the components of circular polarization when light propagates through two gyrotropic crystals.
Assessing the Potential Distribution of Oxalis latifolia, a Rapidly Spreading Weed, in East Asia under Global Climate Change
Authors: Poudel Anil , Adhikari Pradeep , Na Chae Sun , Wee June , Lee Do-Hun , Lee Yong Ho , Hong Sun Hee
Published: September 2023

Oxalis latifolia, a perennial herbaceous weed, is a highly invasive species that poses a threat to agricultural lands worldwide. East Asia is under a high risk of invasion of O. latifolia under global climate change. To evaluate this risk, we employed maximum entropy modeling considering two shared socio-economic pathways (SSP2-4.5 and SSP5-8.5). Currently, a small portion (8.02%) of East Asia is within the O. latifolia distribution, with the highest coverages in Chinese Taipei, China, and Japan (95.09%, 9.8%, and 0.24%, respectively). However, our projections indicated that this invasive weed will likely be introduced to South Korea and North Korea between 2041 and 2060 and 2081 and 2100, respectively. The species is expected to cover approximately 9.79% and 23.68% (SSP2-4.5) and 11.60% and 27.41% (SSP5-8.5) of the total land surface in East Asia by these time points, respectively. South Korea and Japan will be particularly susceptible, with O. latifolia potentially invading up to 80.73% of their territory by 2081–2100. Mongolia is projected to remain unaffected. This study underscores the urgent need for effective management strategies and careful planning to prevent the introduction and limit the expansion of O. latifolia in East Asian countries.
Facile Preparation of Carbon Nanotubes/Cellulose Nanofibrils/Manganese Dioxide Nanowires Electrode for Improved Solid-Sate Supercapacitor Performances
Authors: Chin Siew Xian , Lau Kam Sheng , Ginting Riski Titian , Tan Sin Tee , Khiew Poi Sim , Chia Chin Hua , Wongchoosuk Chatchawal
Published: September 2023

Wearable energy storage devices require high mechanical stability and high-capacitance flexible electrodes. In this study, we design a flexible supercapacitor electrode consisting of 1-dimensional carbon nanotubes (CNT), cellulose nanofibrils (CNF), and manganese dioxide nanowires (MnO2 NWs). The flexible and conductive CNT/CNF-MnO2 NWs suspension was first prepared via ultrasonic dispersion approach, followed by vacuum filtration and hot press to form the composite paper electrode. The morphological studies show entanglement between CNT and CNF, which supports the mechanical properties of the composite. The CNT/CNF-MnO2 NWs electrode exhibits lower resistance when subjected to various bending angles (−120–+120°) compared to the CNT/CNF electrode. In addition, the solid-state supercapacitor also shows a high energy density of 38 μWh cm−2 and capacitance retention of 83.2% after 5000 cycles.
Experimental and Computational Fluid Dynamic—CFD Analysis Simulation of Heat Transfer Using Graphene Nanoplatelets GNP/Water in the Double Tube Heat Exchanger
Authors: Lima Carlos C. X. S. , Ochoa Alvaro A. V. , da Costa José A. P. , de Menezes Frederico D. , Alves João V. P. , Ferreira Julia M. G. A. , Azevedo Clara C. A. , Michima Paula S. A. , Leite Gustavo N. P.
Published: September 2023

This study investigates and compares the experimental heat transfer performance and simulation via computational fluid dynamics (CFD) of graphene nanoplatelets (GNP) and water nanofluids GNP/water in the double-tube-type heat exchanger (DTHE). Tests were conducted with water/water and GNP/water fluids, with the nanofluid for the hot-fluid circuit and water for the cold-fluid circuit, with counterflow direction, varying the nanofluid concentrations by weight (wt%) at 0.0125%, 0.025%, and 0.050%, the operating temperature at 50 and 60 °C, and Reynolds numbers between 2000–6000. The results showed that 0.025 wt% GNP presented better thermal performance, with a 28% increase in the temperature gain. The 0.025 wt% GNP had slightly better performance for the Nusselt number (Nu), and the 0.05 wt% GNP had a slightly better thermal effectiveness. The comparison between the experimental values showed good agreement with those calculated by empirical correlations and the CFD model, with maximum and minimum relative error values of 9% and 1%, respectively, when the Petukhov equation was used.
Research on a Carbon Emission Prediction Method for Oil Field Transfer Stations Based on an Improved Genetic Algorithm—The Decision Tree Algorithm
Authors: Cheng Qinglin , Wang Xue , Wang Shuang , Li Yanting , Liu Hegao , Li Zhidong , Sun Wei
Published: September 2023

The background of “dual carbon” is accelerating low-carbon transformation in the energy field, and oil field enterprises are facing challenges in energy conservation and emissions reduction for sustainable development. However, oil field gathering and transfer station systems, which are crucial components of the onshore transportation system, face challenges in energy conservation and emissions reduction. Therefore, it is necessary to predict the carbon emissions of oil field gathering and transfer station systems. To improve the accuracy of carbon emission prediction for the system, this study proposes an improved GA-decision tree (IGA-decision tree) algorithm. First, chaotic mapping was introduced to initialize the population, ensuring a uniform distribution of initial particles in the search space and enhancing population diversity. Second, the firefly perturbation strategy was employed to avoid the problem of genetic algorithms becoming trapped in local optima during the later stages of the search. The results show that the enhanced GA-decision tree algorithm effectively avoided being stuck in local optima while performing global searches. When predicting the carbon emissions of oil field gathering and transfer stations, the improved GA-decision tree (IGA-decision tree) algorithm outperformed traditional decision tree and GA-decision tree algorithms in terms of error and convergence efficiency. It achieved a root mean square error (RMSE) value of 74.5181 and a correlation coefficient (R2) of 0.99, indicating a high level of fitness and good convergence, as well as high prediction accuracy. This algorithm contributes to carbon accounting and energy conservation efforts in oilfield gathering and the transfer station system, filling the research gap in carbon emissions prediction for the system within the framework of energy internet projects.
Exploring Partial Structural Disorder in Anhydrous Paraxanthine through Combined Experiment, Solid-State Computational Modelling, and Molecular Docking
Authors: Latosińska Jolanta Natalia , Latosińska Magdalena , Seliger Janez , Žagar Veselko
Published: September 2023

Paraxanthine (PX), a major metabolite of caffeine, a protective agent against Alzheimer’s and Parkinson’s disease, and a promising drug for the treatment of post-COVID 2019 anosmia and ageusia, has been studied in the solid state and protein–ligand complex. Partial disorder in PX, caused by the methyl group at the N(7) position, has been modelled and discussed. The relationship between the unusual structural disorder and the propensity to form a specific system of non-covalent bonds was analyzed. Three 1H-14N NMR-NQR (nuclear magnetic resonance–nuclear quadrupole resonance) experimental techniques were used, namely multiple frequency sweeps, Larmor frequency scanning, and the two-frequency irradiation, followed by solid-state computational modelling (density functional theory, supplemented by quantum theory of atoms in molecules, 3D Hirshfeld surfaces, and reduced density gradient), and molecular docking approaches. New quantitative methods for estimating changes in the global pattern of interactions under the influence of rotation of the methyl group in N(7) based on the Pompeiu–Hausdorff and Bhattacharayya metrics and the Wasserstein distance have been proposed and applied. A spectrum consisting of 12 lines, indicating the presence of 4 chemically inequivalent nitrogen sites in the PX molecule, was recorded, and the lines’ assignment to particular sites was made. The influence of the methyl rotation on the eigenvalues and eigenvectors of the electric field gradient tensor, NQR parameters, and resonance line positions was modelled in the solid (GGA/RPBE, m-GGA/RSCAN) and cluster (Minnesota M062X hybrid). Three factors have been found to determine structural disorder in PX: larger crystal voids near the methyl at N(7) than at N(1) (opening the path for the disorder), hyperconjugation strongly affecting the density distribution in the five-membered ring, and the involvement of the methyl group at N(7) in many non-covalent bonds that intercept (capture) subsequent jumping protons. The Pompeiu–Hausdorff and Bhattacharayya metrics and the Wasserstein distance confirmed the changes in the distribution and strength of non-covalent interactions throughout the molecule as a result of methyl rotation. This effect is clearly visible regardless of the type of metric, and its order of magnitude is consistent with the modulation effect of the NQR spectra (experimental and calculated). Through molecular docking, it was discovered that the PX moiety in protein–ligand complexes adopt the same methyl group conformation at N(7) as in the solid state. It was found that the cooperation–competition between the C-H⋯O hydrogen bonds and C-H⋯H-C dispersion interactions is the crucial factor that impedes methyl rotation and induces structural disorder, as well as being an important factor in the formation of the protein–ligand complexes.
Monitoring the Ignition of Hay and Straw by Radiant Heat
Authors: Markova Iveta , Giertlova Zuzana , Jadudova Jana , Turekova Ivana
Published: September 2023

Hay and straw are commonly used materials in agriculture. They are organic materials and, therefore, flammable. This article examines the behaviour of hay and straw when exposed to radiant heat. The objective of this study is to experimentally determine the ignition temperature of hay and straw under the influence of radiant heat. This research investigates the effects of sample type (hay and straw) and sample quantity on the thermal degradation process, temperature increase within the samples, and ignition temperature of the samples as a function of time. The ignition temperature of hay was determined to be higher (407 °C) compared to straw (380 °C).
Statistical Process Control Using Control Charts with Variable Parameters
Authors: Sałaciński Tadeusz , Chrzanowski Jarosław , Chmielewski Tomasz
Published: September 2023

An extremely important issue in quality management is monitoring and diagnosing processes, and, subsequently, supervising them using so-called control charts. In typical production processes, charts with constant parameters are commonly used, such as x-R, x-s, CUSUM, EWMA and others, which, in most cases, are effective tools for process stability evaluation. Charts considered untypical (in statistical process control) are those with variable sample sizes, variable sampling intervals and/or variable control limits. Such charts are used when process analysis based on standard, well-known charts may lead to serious errors. Modern control charts are a response to the requirements of Industry 4.0 and are an excellent tool for supervising production processes. Their use together with Cp and Cpk indices and other process capability indices is a starting point for process improvement. The methodology of nonstandard charts is inadequately recognized and rarely used in practice. The theory of their design and examples of their use will be presented and characterized in this paper.
“That Further by Faith”: Ancestral Futurity, Reincarnation, and the Conjuration of Denmark Vesey’s Revolutionary Religious Perspective
Authors: Butts Jimmy Earl
Published: September 2023

This article expresses the importance and theoretical viability of Black religious communities reflecting on armed struggle as an option in their pursuit of liberation. African Americans have wrestled with various perspectives on what forms of resistance to white supremacy were religiously legitimate and those that were deemed practical. From moral suasion, immigration, a Black separate state, to violent resistance, Black people in the United States have debated these perspectives and have charted paths forward that continue to be accompanied by Black suffering and death at the hands of racists to the present day. While moral suasion has obtained a hegemonic place in mainstream Black political discourse, violent resistance has often been characterized as both religiously illegitimate and impractical. However, by using concepts from Afrofuturism and traditional African religion, the author will present Denmark Vesey as a model for contemporary Black religio-political thought. Using the themes of “past future”, time travel, resurrection, reincarnation, trance, and conjure, the author grounds himself in an African-centered epistemology that transcends the limitations of the Eurocentric model limited only to scientific “reality”. The author claims that by conjuring Vesey’s revolutionary interreligious Pan-African approach, it will provide more options for Black religio-political theory and praxis.
Assessing the Impact of 20 mph Speed Limits on Vehicle Speeds in Rural Areas: The Case of the Scottish Borders
Authors: Olowosegun Adebola , Fountas Grigorios , Davis Adrian
Published: September 2023

This paper aims at delivering new empirical evidence as to the effectiveness of 20 mph speed limits in rural areas. For this purpose, speed and traffic data were drawn from the area of the Scottish Borders, UK, where the local Council led the rollout of a 20 mph speed limit trial in 97 villages and towns from October 2020. This intervention is considered as one of the first of its kind in the UK and overseas, as it was carried out on a large scale, in predominantly rural areas. To evaluate the impact of the 20 mph speed limit on vehicle speeds, we conducted a “before–after” quantitative analysis using traffic and speed data collected in different waves before and after the intervention. The descriptive analysis showed that both mean and 85th percentile speeds reduced directly after the introduction of the 20 mph speed limit (by 3.1 mph and 3.2 mph, respectively), and that such speed reductions were largely maintained even up to eight months after the onset of the intervention. The largest speed reductions were observed in locations with high-speed patterns before the intervention, and especially in those having mean speeds greater than 25 mph before the intervention. Both non-parametric and parametric statistical tests, which were conducted using approximately five million speed observations, showed that the observed speed changes were statistically significant for the vast majority of cases. Linear regression models were also estimated confirming the significant impact of the 20 mph limit on vehicle speeds, while controlling for the influence of traffic volume. Overall, the findings of this study will likely assist in filling an evidence gap regarding the effectiveness of 20 mph speed limits in rural settlements. They can also provide encouragement to those local authorities in the UK and abroad that are currently actively examining the possibility of setting the 20 mph as the default limit in built-up areas.
Analysis of the Problem-Based Learning Model’s Application for the Sustainable Development of Sports Education
Authors: Jang Ju-Sun
Published: September 2023

The purpose of this study was to analyze importance and performance levels by applying the problem-based learning (PBL) education model for the sustainable development of sports education. In this study, 331 university sports majors who received PBL education at least once were administered a questionnaire from 15 July to 20 September 2022. Frequency analysis (using SPSS 25.0), exploratory factor analysis, reliability analysis, descriptive statistical analysis, and IPA were used for data processing. The results are as follows: The first quadrant of the IPA matrix comprised attributes such as determining the cause of the problem, acquiring sufficient knowledge through learning, gathering information related to the problem, getting to the heart of the matter, and building intimacy among team members. The second quadrant included planning for oneself when learning, considering the importance of self-learning, and actively answering questions. The third quadrant included enthusiasm and sincerity toward team learning. The fourth quadrant included 11 attributes: proper planning and execution, understanding others’ points of view, the joy of team learning, prioritizing problem-solving methods, creative communication, communication with team members, proactive communication, everyone on the team working hard, feeling a sense of accomplishment, a sense of challenge, and an increase in self-confidence.
Merging Design Thinking into Translational Research in a Biomedical Engineering Laboratory (DT-TRBEL) Course
Authors: Chen Min-Hua , Cheng Shih-Ping , Wu Leon Yufeng
Published: September 2023

Laboratory classes offered in universities often fail to develop students’ ability to identify questions and encourage creativity to solve authentic problems. Lab exercises tend to provide clear step-by-step instructions, leaving little room for experimentation or creative thinking. Unfortunately, this approach can result in engineering students losing the skills they need to solve unprecedented challenges in their future professional careers. Biomedical engineering is particularly vulnerable to this training approach, given that students are taught to devise ideas to solve medical problems. To address this issue, the current study combined the curriculum designs of translational research and design thinking. This guided students in bringing biomaterials into the clinic and stimulated their interest in biomaterial development. The resulting course, called DT-TRBEL (Design-Thinking: Translational Research in Biomedical Engineering Laboratory Course), focuses on developing dental biomaterials, including material preparation, analysis, and cytotoxicity testing. The data was collected and evaluated through a survey of self-efficacy of creativity, student motivation, and learning scores of both the prerequisite course “Material Science” and DT-TRBEL. The study found that DT-TRBEL did not have a positive effect on overall motivation or the sense of self-efficacy regarding creativity. However, it did have a significant gender effect, benefiting female students more than male students. The discussion covers implementation and further directions for research.
Acceptability of HPV Vaccines: A Qualitative Systematic Review and Meta-Summary
Authors: Urrutia María-Teresa , Araya Alejandra-Ximena , Gajardo Macarena , Chepo Macarena , Torres Romina , Schilling Andrea
Published: September 2023

In 2006, the human papillomavirus (HPV) vaccine was approved for use as an effective intervention for reducing the risk of developing cervical cancer; however, its successful implementation is dependent on acceptability. This study aims to provide a comprehensive understanding of the reasons that favor or do not favor the acceptability of HPV vaccines. Methods: We conducted a systematic review and meta-summary of qualitative research on 16 databases. A total of 32 articles that considered the perspectives of vaccine users, their parents, and the professionals who care for them were reviewed. Synthesis was conducted as described by Sandelowski and Barroso. Results: We used inductive and deductive methods to obtain a total of 22 dimensions, out of which three issues stood out that should be considered to improve acceptability and are formed by three groups of study, namely, information about the vaccine, fears and side effects, and sexuality associated with the vaccine. Conclusions: Acceptability, as well as adherence to HPV vaccination, is a complex concept. This review highlights the perspectives of the three sets of actors involved in the process (i.e., users, parents, and professionals) and views these factors in relation to acceptability as a guide for new interventions.
Exploring the Relationship between Neutrophil Activation and Different States of Canine L. infantum Infection: Nitroblue Tetrazolium Test and IFN-γ
Authors: Blasi-Brugué Carles , Martínez-Flórez Icíar , Baxarias Marta , del Rio-Velasco Joan , Solano-Gallego Laia
Published: September 2023

This study aimed to investigate the role of neutrophils in canine leishmaniosis by assessing neutrophil activation and its relationship with different states of L. infantum infection and antibody and IFN-γ production. Dogs were categorized into five groups: healthy-seronegative (n = 25), healthy-seropositive (n = 21), LeishVet-stage I (n = 25), Leishvet-stage II (n = 41), and LeishVet-stage III–IV (n = 16). Results of the nitroblue tetrazolium reduction test (NBT) showed significantly higher neutrophil activation in stage I (median:17.17, range: [7.33–31.50]%) compared to in healthy-seronegative (4.10 [1.20–18.00]%), healthy-seropositive (7.65 [3.98–21.74]%), stage II (6.50 [1.50–28.70]%), and stage III–IV (7.50 [3.00–16.75]%) groups (p < 0.0001). Healthy-seropositive dogs also displayed higher values than all groups except stage I. Stages II and III–IV did not show significant differences compared to healthy-seronegative. Regarding IFN-γ, stage I dogs had higher concentrations (median:127.90, range: [0–3998.00] pg/mL) than healthy-seronegative (0 [0–109.50] pg/mL) (p = 0.0002), stage II (9.00 [0–5086.00] pg/mL) (p = 0.045), and stage III–IV (3.50 [80.00–548.80] pg/mL) (p = 0.02) dogs. Stage II dogs showed increased IFN-γ compared to healthy-seronegative dogs (p = 0.015), while stage III–IV dogs had no significant differences compared to healthy-seronegative dogs (p = 0.12). Healthy-seropositive dogs had elevated IFN-γ concentrations compared to healthy-seronegative dogs (p = 0.001) and dogs in stage III–IV (p = 0.03). In conclusion, neutrophil activation was higher in dogs with mild disease and healthy-seropositive dogs, and a relationship between neutrophil activation and the production of IFN-γ was found.
Digitalization in healthcare: open opportunities and hidden risks
Authors:   Federal State Budgetary Educational Institution of Higher Education “Russian Economic University named after G. Plekhanov”, Ministry of Science and Education of the Russian Federation, Moscow, Russia, Lebedev V.A. , Lebedeva E.I. ,   Non-profit Partnership “Institute of Professional Accountants and Auditors of Russia”, Moscow, Russia
Published: September 2023

The issues of digitalization in the field of healthcare in the activities of medical institutions are considered. The increase and complication of the IT equipment of medical institutions should be accompanied not only by increased attention of the employer to the accounting of IT facilities, but also by compliance with legal norms to ensure the safety of these technical means, including the rights of employees entrusted with these facilities. The analysis of the mechanism of financial responsibility of employees of medical institutions, restrictions and risks of the employer is carried out; the assessment of the counter obligations of the parties to the employment contract is given, including the employee to take care of the employer’s property, and the employer to provide appropriate conditions for the employee to perform his work duties.
Change of paradigms in popular culture on the example of the concepts of "maiden-in-distress" and "maiden-warrior"
Authors:   Moscow University for the Humanities, Podobedova Anastasiya Alekseevna
Published: September 2023

The article deals with two popular concepts in modern mass culture - "maiden-in-distress" and "maiden-warrior". These concepts are basic in the construction and creation of a product of mass culture. Meanwhile, there is a greater interest in the "maiden-warrior" phenomenon than in its opposite, "maiden-in-distress". The author shows that the study of femininity, feminine, the woman and her role and place in modern society and its interpretation in modern popular and mass culture is of scientific interest for researchers in literary criticism, cultural studies, philosophy, and sociology. The article provides examples of mass culture products where these archetypal images are used, as well as film and television adaptations of modern female images. It is shown how their interpretation is influenced by public opinion and modern and mainstream trends, which, most often, offer a "different vision" for ordinary and traditional things. The materials of the article can be useful within the framework of areas related to philosophy, history, and cultural studies.
Consciousness and the unconscious: structures, levels, and functions
Authors:   Institute for Fundamental and Applied Research, Moscow University for the Humanities, Voskoboinikov Anatoliy Emmanuilovich
Published: September 2023

The article presents important materials on the course "Concepts of Modern Natural Science", related to the problem of the conscious and the unconscious, their features of manifestation, and connection with human life. The author shows that the more developed the system is, the more important the internal mechanisms of development are for it, which, in turn, are based on past experience and on the growing role of foresight and design of the future. The phylogenetically innate unconscious (the innermost unconscious) is at the heart of human mental life. The ontogenetically formed unconscious (the subconscious) is most directly connected with it. Forming a single whole, these two types of the unconscious serve as the foundation for preconsciousness and consciousness. The materials of the article can be useful within the framework of such areas of study as Psychology, Medicine, and Philosophy.
International legal foundations of criminal proceedings against minors
Authors:   Moscow University for the Humanities, Vorobiev Vladimir Yurievich
Published: September 2023

The article deals with the international legal participation of a minor in criminal proceedings. It is shown that age-related vulnerability and immaturity inherent in minors require additional legal guarantees in criminal proceedings. The personal characteristics of juvenile criminally prosecuted persons, victims, and witnesses and their limited procedural capacity can be considered as sufficient grounds for involving additional participants in the criminal process. Objectively, there is a need for legal and individual regulation of the relevant criminal procedural relations in law enforcement.