bluuebunny/crossref_metadata_2025 huggingface dataset:
CRISPR/Cas9-Mediated SlMYBS2 Mutagenesis Reduces Tomato Resistance to Phytophthora infestans
Authors: Liu Chunxin , Zhang Yiyao , Tan Yinxiao , Zhao Tingting , Xu Xiangyang , Yang Huanhuan , Li Jingfu
Published: October 2021

Phytophthora infestans (P. infestans) recently caused epidemics of tomato late blight. Our study aimed to identify the function of the SlMYBS2 gene in response to tomato late blight. To further investigate the function of SlMYBS2 in tomato resistance to P. infestans, we studied the effects of SlMYBS2 gene knock out. The SlMYBS2 gene was knocked out by CRISPR-Cas9, and the resulting plants (SlMYBS2 gene knockout, slmybs2-c) showed reduced resistance to P. infestans, accompanied by increases in the number of necrotic cells, lesion sizes, and disease index. Furthermore, after P. infestans infection, the expression levels of pathogenesis-related (PR) genes in slmybs2-c plants were significantly lower than those in wild-type (AC) plants, while the number of necrotic cells and the accumulation of reactive oxygen species (ROS) were higher than those in wild-type plants. Taken together, these results indicate that SlMYBS2 acts as a positive regulator of tomato resistance to P. infestans infection by regulating the ROS level and the expression level of PR genes.
Molecular Karyotyping on Populus simonii × P. nigra and the Derived Doubled Haploid
Authors: Liu Bo , Wang Sui , Tao Xiaoyan , Liu Caixia , Qu Guanzheng , Dou Quanwen
Published: October 2021

The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid Populus simonii × P. nigra (P. xiaohei) and the derived doubled haploids was carried out with labeled telomeres, rDNA, and two newly repetitive sequences as probes by fluorescence in situ hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with high-sequence homology were used, and the results showed that they presented the colocalized distribution signal in chromosomes. For P. xiaohei, pPD284_XHY produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six pairs of chromosomes, and the three pairs of chromosomes showed a significant difference in the hybridization between homologous chromosomes. The repeat probes used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY generated an additional hybridization site in the telomere region of chromosome 14. Moreover, two pairs of chromosomes showed differential hybridization distributions between homologous chromosomes. Comparisons of the distinguished chromosomes between hybrid and DH poplar showed that three pairs of chromosomes in the DH presented hybridization patterns that varied from those of the hybrid. The No. 8 chromosome in DH and one of the homologous chromosomes in P. xiaohei shared highly similar FISH patterns, which suggested the possibility of intact or mostly partial transfer of the chromosome between the hybrid and DH. Our study will contribute to understanding the genetic mechanism of chromosomal variation in P. xiaohei and derived DH plants.
Developing a Phototactic Electrostatic Insect Trap Targeting Whiteflies, Leafminers, and Thrips in Greenhouses
Authors: Takikawa Yoshihiro , Nonomura Teruo , Sonoda Takahiro , Matsuda Yoshinori
Published: October 2021

Our aim was to develop an electrostatic apparatus to lure and capture silverleaf whiteflies (Bemisia tabaci), vegetable leafminers (Liriomyza sativae), and western flower thrips (Frankliniella occidentalis) that invade tomato greenhouses. A double-charged dipolar electric field producer (DD-EFP) was constructed by filling water in two identical transparent soft polyvinyl chloride tubes arrayed in parallel with fixed separation, and then, inserting the probes of grounded negative and positive voltage generators into the water of the two tubes to generate negatively and positively charged waters, respectively. These charged waters electrified the outer surfaces of the opposite tubes via dielectric polarization. An electric field formed between the oppositely charged tubes. To lure these phototactic insects, the water was colored yellow using watercolor paste, then introduced into the transparent insulator tubes to construct the yellow-colored DD-EFP. This apparatus lured insects in a manner similar to commercially available yellow sticky traps. The yellow-colored DD-EFP was easily placed as a movable upright screen along the plants, such that invading pests were preferentially attracted to the trap before reaching the plants. Furthermore, pests settling on the plants were attracted to the apparatus, which used a plant-tapping method to drive them off the plants. Our study provided an experimental basis for developing an electrostatic device to attract and capture insects that enter greenhouses.
Sepsis and the Human Microbiome. Just Another Kind of Organ Failure? A Review
Authors: Tourelle Kevin M. , Boutin Sebastien , Weigand Markus A. , Schmitt Felix C. F.
Published: October 2021

Next-generation sequencing (NGS) has been further optimised during the last years and has given us new insights into the human microbiome. The 16S rDNA sequencing, especially, is a cheap, fast, and reliable method that can reveal significantly more microorganisms compared to culture-based diagnostics. It might be a useful method for patients suffering from severe sepsis and at risk of organ failure because early detection and differentiation between healthy and harmful microorganisms are essential for effective therapy. In particular, the gut and lung microbiome in critically ill patients have been probed by NGS. For this review, an iterative approach was used. Current data suggest that an altered microbiome with a decreased alpha-diversity compared to healthy individuals could negatively influence the individual patient’s outcome. In the future, NGS may not only contribute to the diagnosis of complications. Patients at risk could also be identified before surgery or even during their stay in an intensive care unit. Unfortunately, there is still a lack of knowledge to make precise statements about what constitutes a healthy microbiome, which patients exactly have an increased perioperative risk, and what could be a possible therapy to strengthen the microbiome. This work is an iterative review that presents the current state of knowledge in this field.
Comparing Humoral and Cellular Adaptive Immunity during Convalescent Phase of COVID-19 in Hemodialysis Patients and Kidney Transplant Recipients
Authors: Kamińska Dorota , Augustyniak-Bartosik Hanna , Kościelska-Kasprzak Katarzyna , Żabińska Marcelina , Bartoszek Dorota , Poznański Paweł , Kuriata-Kordek Magdalena , Kusztal Mariusz , Mazanowska Oktawia , Krajewska Magdalena
Published: October 2021

Background. It is still unclear whether COVID-19 convalescent kidney transplant recipients (KTR) and hemodialysis (HD) patients can develop anti-SARS-CoV-2 adaptive immunity. The aim was to characterize and compare the immune response to the virus in HD patients and KTR. Methods. The study included 26 HD patients and 54 KTR—both convalescent (14 HD, 25 KTR) and unexposed. The immune response was assessed by determining the anti-SARS-CoV-2 antibodies in serum and specific T cell response via the interferon-gamma release assay (IGRA). Moreover, blood-morphology-derived parameters, immune cell phenotypes, and acute phase reactants were evaluated. Results. KRT and HD convalescents presented similar serum levels of anti-SARS-CoV-2 IgG and IgA. A negative correlation occurred between IgG and time after the infection was observed. There was a strong relationship between the prevalence of anti-SARS-CoV-2 cellular and humoral responses in both groups. Convalescent IGRA response was significantly higher in HD patients compared to KTR. Conclusions. HD patients and KTR develop humoral and cellular responses after COVID-19. The antibodies levels are similar in both groups of patients. SARS-CoV-2-reactive T cell response is stronger in HD patients compared to KTR. The SARS-CoV-2-specific IgG level decreases with time while IgA and a cellular response are maintained. IGRA proved to be a valuable test for the assessment of specific cellular immunity in immunocompromised HD patients and KTR.
Prognostic Difference of Pleural versus Distant Metastasis after Surgery for Lung Cancer
Authors: Joubert Kyla D. , Okusanya Olugbenga T. , Mazur Summer , Ryan John P. , Ekeke Chigozirim N. , Schuchert Matthew J. , Soloff Adam C. , Dhupar Rajeev
Published: October 2021

Background: Pleural metastasis in lung cancer found at diagnosis has a poor prognosis, with 5–11 months’ survival. We hypothesized that prognosis might be different for patients who have had curative-intent surgery and subsequent pleural recurrence and that survival might differ based on the location of the first metastasis (distant versus pleural). This may clarify if pleural recurrence is a local event or due to systemic disease. Methods: A database of 5089 patients who underwent curative-intent surgery for lung cancer was queried, and 85 patients were found who had biopsy-proven pleural metastasis during surveillance. We examined survival based on pattern of metastasis (pleural first versus distant first/simultaneously). Results: Median survival was 34 months (range: 1–171) from the time of surgery and 13 months (range: 0–153) from the time of recurrence. The shortest median survival after recurrence was in patients with adenocarcinoma and pleural metastasis as the first site (6 months). For patients with pleural metastasis as the first site, those with adenocarcinoma had a significantly shorter post-recurrence survival when compared with squamous cell carcinoma (6 vs. 12 months; HR = 0.34) and a significantly shorter survival from the time of surgery when compared with distant metastases first/simultaneously (25 vs. 52 months; HR = 0.49). Conclusions: Patients who undergo curative-intent surgery for lung adenocarcinoma that have pleural recurrence as the first site have poor survival. This may indicate that pleural recurrence after lung surgery is not likely due to a localized event but rather indicates systemic disease; however, this would require further study.
Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
Authors: Urbano Nicoletta , Scimeca Manuel , Tolomeo Anna , Dimiccoli Vincenzo , Bonanno Elena , Schillaci Orazio
Published: October 2021

In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into three groups according to post-injection time points of an 18F-PSMA inhibitor. Groups of 4 mice were used to evaluate the biodistribution of the radiopharmaceutical after 30-, 60- and 120-min post-injection. Data here reported demonstrated that the digital autoradiography system is suitable to analyse the biodistribution of an 18F-PSMA inhibitor in both whole small-animal bodies and in single organs. The exposure of both whole mouse bodies and organs on the super resolution screen surface allowed the radioactivity of the PSMA inhibitor distributed in the tissues to be detected and quantified. Data obtained by using a digital autoradiography system were in line with the values detected by the activity calibrator. In addition, the image obtained from the super resolution screen allowed a perfect overlap with the tumour images achieved under the optical microscope. In conclusion, biodistribution studies performed by the autoradiography system allow the microscopical modifications induced by therapeutic radiopharmaceuticals to be studied by comparing the molecular imaging and histopathological data at the sub-cellular level.
IgA Vasculitis with Nephritis in Adults: Histological and Clinical Assessment
Authors: Lai Lingyun , Liu Shaojun , Azrad Maria , Hall Stacy , Hao Chuanming , Novak Jan , Julian Bruce A. , Novak Lea
Published: October 2021

Patients with IgA vasculitis (IgAV), an immune complex-mediated disease, may exhibit kidney involvement—IgAV with nephritis (IgAVN). The kidney-biopsy histopathologic features of IgAVN are similar to those of IgA nephropathy, but little is known about histopathologic disease severity based on the interval between purpura onset and diagnostic kidney biopsy. We assessed kidney histopathology and clinical and laboratory data in a cohort of adult patients with IgAVN (n = 110). The cases were grouped based on the interval between the onset of purpura and kidney biopsy: Group 1 (G1, <1 month, n = 14), Group 2 (G2, 1–6 months, n = 58), and Group 3 (G3, >6 months, n = 38). Glomerular leukocytes were more common in G1 than in the other groups (p = 0.0008). The proportion of neutrophils among peripheral-blood leukocytes was the highest in the patients biopsied within a month after onset of purpura (G1: 71 ± 8%). In the patients with an interval >6 months, the neutrophil proportion was lower, 60%. Moreover, the glomerular mesangial proliferation score correlated with the serum total IgA concentration (p = 0.0056). In conclusion, IgAVN patients biopsied <1 month from purpura onset showed an elevated percentage of blood neutrophils and glomerular leukocytes, consistent with an acute-onset inflammatory reaction. In all IgAVN patients, the mesangial proliferation score correlated with the serum IgA level.
Myocardial Aldosterone Receptor and Aquaporin 1 Up-Regulation Is Associated with Cardiomyocyte Remodeling in Human Heart Failure
Authors: Frustaci Andrea , Letizia Claudio , Chimenti Cristina , Verardo Romina , Alfarano Maria , Scialla Rossella , Bagnato Giulia , Miraldi Fabio , Sansone Luigi , Russo Matteo Antonio
Published: October 2021

Background: Abnormal aldosterone signaling is a recognized source of cardiovascular damage. Its influence on cardiomyocyte structure, function, and hormonal receptors when associated with heart failure is still unreported. Methods: Twenty-six consecutive patients with heart failure (LVEF < 40%) and normal coronaries and valves underwent left ventricular endomyocardial biopsy (EMB) for evaluation of myocardial substrate. Biopsy samples were processed for histology, electron microscopy, immunohistochemistry, and Western blot analysis of myocardial aldosterone receptor and aquaporin-1 correlated with plasma aldosterone (AD) and renin activity (PRA). Eight patients with virus-negative inflammatory cardiomyopathy (ICM) had a control EMB after 6 months of immunosuppressive therapy and recovery of cardiac function with re-evaluation of cardiomyocyte structure and receptor expression. Results: EMB in addition to the diagnosis of myocarditis (15 cases), dilated cardiomyopathy CM (6), alcohol CM (2), and diabetic CM (3) showed vacuolar degeneration and cloudy swelling of cardiomyocytes corresponding at electron microscopy to ions and water accumulation into cytosol, membrane-bound vesicles, nucleus, and other organelles, and was associated with an increased AD, PRA, and myocardial expression of aldosterone receptor (2.6 fold) and aquaporin 1 (2.7 fold). In the 8 patients recovered from ICM, cardiomyocyte diameter reduced with disappearance of intracellular vacuoles and normalization of cytosol, nucleus, and cell organelles’ electron-density, along with down-regulation of aldosterone receptor and aquaporin-1. Conclusion: Human heart failure is associated with overexpression of myocardial aldosterone receptor and aquaporin-1. These molecular changes are paralleled by intracellular water overloading and cardiomyocyte swelling and dysfunction. Cardiac recovery is accompanied by down-regulation of hormonal receptors and normalization of cell structure and composition.
Altered mRNA Expression of Interleukin-1 Receptors in Myocardial Tissue of Patients with Left Ventricular Assist Device Support
Authors: Niazy Naima , Mrozek Linus , Barth Mareike , Immohr Moritz Benjamin , Kalampokas Nikolaos , Saeed Diyar , Aubin Hug , Sugimura Yukiharu , Westenfeld Ralf , Boeken Udo , Lichtenberg Artur , Akhyari Payam
Published: October 2021

Serum levels of cytokines interleukin 1 beta ( IL-1β) and interleukin 33 (IL-33) are highly abnormal in heart failure and remain elevated after mechanical circulatory support (MCS). However, local cytokine signaling induction remains elusive. Left (LV) and right ventricular (RV) myocardial tissue specimens of end-stage heart failure (HF) patients without (n = 24) and with MCS (n = 39; 594 ± 57 days) were analyzed for cytokine mRNA expression level of IL-1B, interleukin 1 receptor 1/2 (IL-1R1/2), interleukin 1 receptor-like 1 (IL-1RL1), IL-33 and interleukin-1 receptor accessory protein (IL-1RaP). MCS patients showed significantly elevated IL-1B expression levels (LV: 2.0 fold, p = 0.0058; RV: 3.3 fold, p < 0.0001). Moreover, IL-1R1, IL-1RaP and IL-33 expression levels strongly correlated with each other. IL-1RL1 and IL-1R2 expression levels were significantly higher in RV myocardial tissue (RV/LV ratio IL-1R2 HF: 4.400 ± 1.359; MCS: 4.657 ± 0.655; IL-1RL1 HF: 3.697 ± 0.876; MCS: 4.529 ± 0.5839). In addition, IL1-RaP and IL-33 RV expression levels were significantly elevated in MCS. Furthermore, IL-33 expression correlates with C-reactive protein (CRP) plasma levels in HF, but not in MCS patients. Increased expression of IL-1B and altered correlation patterns of IL-1 receptors indicate enhanced IL-1β signaling in MCS patients. Correlation of IL-1 receptor expression with IL-33 may hint towards a link between both pathways. Moreover, diverging expression in LV and RV suggests specific regulation of local cytokine signaling.
Exploratory Profiling of Extracellular MicroRNAs in Cerebrospinal Fluid Comparing Leptomeningeal Metastasis with Other Central Nervous System Tumor Statuses
Authors: Im Ji Hye , Kim Tae Hoon , Lee Kyue-Yim , Gwak Ho-Shin , Lin Weiwei , Park Jong Bae , Kim Jong Heon , Yoo Byong Chul , Park Seong-Min , Kwon Ji-Woong , Shin Sang Hoon , Yoo Heon
Published: October 2021

The diagnosis of leptomeningeal metastasis (LM) is often difficult due to the paucity of cancer cells in cerebrospinal fluid (CSF) and nonspecific findings on neuroimaging. Investigations of extracellular microRNAs (miRNAs) in CSF could be used for both the diagnosis and study of LM pathogenesis because they reflect the activity of disseminating cancer cells. We isolated CSF extracellular miRNAs from patients (n = 65) of different central nervous system tumor statuses, including cancer control, healthy control, LM, brain metastasis (BM), and primary brain tumor (BT) groups, and performed miRNA microarrays. In unsupervised clustering analyses, all LM and two BM samples showed unique profiles. Among 30 miRNAs identified for LM-specific biomarkers via a Prediction Analysis of Microarrays, miR-335-5p and miR-34b-3p were confirmed in both the discovery and validation samples (n = 23). Next, we performed a significance analysis of the microarray (SAM) to extract discriminative miRNA profiles of two selected CSF groups, with LM samples revealing a greater number of discriminative miRNAs than BM and BT samples compared to controls. Using SAM comparisons between LM and BM samples, we identified 30 upregulated and 6 downregulated LM miRNAs. To reduce bias from different primary cancers, we performed a subset analysis with primary non-small cell lung cancer, and 12 of 13 upregulated miRNAs in LM vs. BM belonged to the upregulated miRNAs in LM. We identified possible target genes and their biological processes that could be affected by LM discriminative miRNAs in NSCLC using the gene ontology database. In conclusion, we identified a unique extracellular miRNA profile in LM CSF that was different from BM, suggesting the use of miRNAs as LM biomarkers in studies of LM pathogenesis.
Occurrence and Morpho-Molecular Identification of Botryosphaeriales Species from Guizhou Province, China
Authors: Dissanayake Asha J. , Chen Ya-Ya , Cheewangkoon Ratchadawan , Liu Jian-Kui
Published: October 2021

Botryosphaeriales is an important order of diverse fungal pathogens, saprobes, and endophytes distributed worldwide. Recent studies of Botryosphaeriales in China have discovered a broad range of species, some of which have not been formerly described. In this study, 60 saprobic isolates were obtained from decaying woody hosts in southwestern China. The isolates were compared with other species using morphological characteristics, and available DNA sequence data was used to infer phylogenetic analyses based on the internal transcribed spacer (ITS), large subunit rRNA gene (LSU), and translation elongation factor 1-α (tef) loci. Three novel species were illustrated and described as Botryobambusa guizhouensis, Sardiniella elliptica, and Sphaeropsis guizhouensis, which belong to rarely identified genera within Botryosphaeriaceae. Botryobambusa guizhouensis is the second species identified from the respective monotypic genus. The previously known species were identified as Aplosporella hesperidica, Barriopsis tectonae, Botryosphaeria dothidea, Diplodia mutila, Di. neojuniperi, Di. pseudoseriata, Di. sapinea, Di. seriata, Dothiorella sarmentorum, Do. yunnana, Lasiodiplodia pseudotheobromae, Neofusicoccum parvum, Sardiniella celtidis, Sa. guizhouensis, and Sphaeropsis citrigena. The results of this study indicate that numerous species of Botryosphaeriales are yet to be revealed in southwestern China.
A Sneak-Peek into the Physician’s Brain: A Retrospective Machine Learning-Driven Investigation of Decision-Making in TAVR versus SAVR for Young High-Risk Patients with Severe Symptomatic Aortic Stenosis
Authors: Hasimbegovic Ena , Papp Laszlo , Grahovac Marko , Krajnc Denis , Poschner Thomas , Hasan Waseem , Andreas Martin , Gross Christoph , Strouhal Andreas , Delle-Karth Georg , Grabenwöger Martin , Adlbrecht Christopher , Mach Markus
Published: October 2021

Transcatheter aortic valve replacement (TAVR) has rapidly become a viable alternative to the conventional isolated surgical aortic valve replacement (iSAVR) for treating severe symptomatic aortic stenosis. However, data on younger patients is scarce and a gap exists between data-based recommendations and the clinical use of TAVR. In our study, we utilized a machine learning (ML) driven approach to model the complex decision-making process of Heart Teams when treating young patients with severe symptomatic aortic stenosis with either TAVR or iSAVR and to identify the relevant considerations. Out of the considered factors, the variables most prominently featured in our ML model were congestive heart failure, established risk assessment scores, previous cardiac surgeries, a reduced left ventricular ejection fraction and peripheral vascular disease. Our study demonstrates a viable application of ML-based approaches for studying and understanding complex clinical decision-making processes.
A Review of Small Farmer Land Use and Deforestation in Tropical Forest Frontiers: Implications for Conservation and Sustainable Livelihoods
Authors: López-Carr David
Published: October 2021

Forest conversion for agriculture is the most expansive signature of human occupation on the Earth’s surface. This paper develops a conceptual model of factors underlying frontier agricultural expansion—the predominant driver of deforestation worldwide—from the perspective of small farm households—the majority of farmers globally. The framework consists of four causal rubrics: demographic, socioeconomic, political–economic, and ecological. Following this approach, the article explores the current state of knowledge on tropical deforestation in tropical agricultural frontiers with a focus on Latin America, the region of greatest deforestation worldwide during recent decades. Neo-Malthusian arguments notwithstanding, in many tropical nations, deforestation has proceeded unabated in recent years despite declining rural populations. However, evidence from the global-to-household scale suggests that population size and composition are also related to farm forest conversion. Existing particularist or behaviorialist theories sometimes fail to capture key geographical and temporal dimensions, yet studies support the notion that certain cultural, individual, and household characteristics are crucial determinants of forest clearing. Conversely, while institutional arguments sometimes fail to emphasize that the ultimate land use change agents are local resource users, their livelihood decisions are shaped and constrained by policies governing economic subsidies, and market and infrastructure development. Further, although ecological change is usually modeled as an outcome in the deforestation literature, increasingly acute climate change and natural farm endowments form a dynamic tabula rasa on which household land use decisions are enabled. To more fully comprehend frontier forest conversion and to enhance protection and conservation while promoting vital local livelihoods, future research may fruitfully investigate the interaction of demographic, social, political, economic, and ecological factors across spatial scales and academic disciplines.
Timing of Tree Density Increases, Influence of Climate Change, and a Land Use Proxy for Tree Density Increases in the Eastern United States
Authors: Hanberry Brice B.
Published: October 2021

Long-term observations inform relationships among changes in vegetation, climate, and land use. For the eastern United States, I compared the timing of tree change, comprised of density and diversity increases, with the timing of climate change, as measured by change point detection of the Palmer Modified Drought Index (PMDI) that accounts for water balance, in two prairie ecological provinces, four grassland landscapes, and four forest landscapes. Historical evidence supplied documentation of tree density increases between approximately 1860 and 1890 in the two prairie provinces of grasslands bordering eastern forests. Additionally, because timing of tree increases paralleled when land area reached ≥25% agricultural use, I categorized grassland and forest landscapes that increased to ≥25% agricultural area during 1860, 1880, 1900, and 1920. One change point detection method identified no significant PMDI change points during the 1800s. The other method found the southern prairie province, bordering eastern forests, had change points of 1855 and 1865 during an interval of relative dryness. Only two of four grassland landscapes, and one of four forest landscapes had change points, which occurred during relative dryness or were continuous with historical variation. Inconsistent changes in moisture availability did not provide correlations with comprehensive tree increases, but land use change corresponded with tree changes based on timing, magnitude and direction of change, and mechanism. The agricultural threshold may provide the critical missing component that allows progression in analysis of land use change effects on vegetation.
Structure, Activity and Function of the Dual Protein Lysine and Protein N-Terminal Methyltransferase METTL13
Authors: Jakobsson Magnus E.
Published: October 2021

METTL13 (also known as eEF1A-KNMT and FEAT) is a dual methyltransferase reported to target the N-terminus and Lys55 in the eukaryotic translation elongation factor 1 alpha (eEF1A). METTL13-mediated methylation of eEF1A has functional consequences related to translation dynamics and include altered rate of global protein synthesis and translation of specific codons. Aberrant regulation of METTL13 has been linked to several types of cancer but the precise mechanisms are not yet fully understood. In this article, the current literature related to the structure, activity, and function of METTL13 is systematically reviewed and put into context. The links between METTL13 and diseases, mainly different types of cancer, are also summarized. Finally, key challenges and opportunities for METTL13 research are pinpointed in a prospective outlook.
In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties
Authors: Grandis Rone Aparecido De , Miotto Larissa Natiele , Genaro Luis Eduardo , Migliatti Polli Larissa , Plepis Ana Maria de Guzzi , Rodrigues Fabiana Tessari , Martins Virginia da Conceição Amaro , Pereira Franchi Leonardo , Scarel-Caminaga Raquel Mantuaneli , Sidorenko de Oliveira Capote Ticiana
Published: October 2021

The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (p > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (p > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.
Softening Effects in Biological Tissues and NiTi Knitwear during Cyclic Loading
Authors: Yasenchuk Yuri F. , Marchenko Ekaterina S. , Gunter Sergey V. , Baigonakova Gulsharat A. , Kokorev Oleg V. , Volinsky Alex A. , Topolnitsky Evgeny B.
Published: October 2021

Samples of skin, tendons, muscles, and knitwear composed of NiTi wire are studied by uniaxial cyclic tension and stretching to rupture. The metal knitted mesh behaves similar to a superelastic material when stretched, similar to soft biological tissues. The superelasticity effect was found in NiTi wire, but not in the mesh composed of it. A softening effect similar to biological tissues is observed during the cyclic stretching of the mesh. The mechanical behavior of the NiTi mesh is similar to the biomechanical behavior of biological tissues. The discovered superelastic effects allow developing criteria for the selection and evaluation of mesh materials composed of titanium nickelide for soft tissue reconstructive surgery.
Metal Matrix Composite in Heat Sink Application: Reinforcement, Processing, and Properties
Authors: Baig Mirza Murtuza Ali , Hassan Syed Fida , Saheb Nouari , Patel Faheemuddin
Published: October 2021

Heat sinks are commonly used for cooling electronic devices and high-power electrical systems. The ever-increasing performance of electronic systems together with miniaturization calls for better heat dissipation. Therefore, the heat sink materials should not only have high thermal conductivities, low densities, and cost, but also have coefficients of thermal expansion matching to those of semiconductor chips and ceramic substrates. As traditional materials fail to meet these requirements, new composite materials have been developed with a major focus on metal matrix composites (MMCs). MMCs can be tailored to obtain the desired combination of properties by selecting proper metallic matrix and optimizing the size and type, volume fraction, and distribution pattern of the reinforcements. Hence, the current review comprehensively summarizes different studies on enhancing the thermal performance of metallic matrices using several types of reinforcements and their combinations to produce composites. Special attention is paid to the types of commonly used metallic matrices and reinforcements, processing techniques adopted, and the effects of each of these reinforcements (and their combinations) on the thermal properties of the developed composite. Focus is also placed on highlighting the significance of interfacial bonding in achieving optimum thermal performance and the techniques to improve interfacial bonding.
Impact of Induction Furnace Steel Slag as Replacement for Fired Clay Brick Aggregate on Flexural and Durability Performances of RC Beams
Authors: Miah Md Jihad , Ali Md. Kawsar , Li Ye , Babafemi Adewumi John , Paul Suvash Chandra
Published: October 2021

This research investigates the flexural and durability performances of reinforced concrete (RC) beams made with induction furnace steel slag aggregate (IFSSA) as a replacement for fired clay brick aggregate (FCBA). To achieve this, 27 RC beams (length: 750 mm, width: 125 mm, height: 200 mm) were made with FCBA replaced by IFSSA at nine replacement levels of 0%, 10%, 20%, 30%, 40%, 50%, 60%, 80%, and 100% (by volume). Flexural tests of RC beams were conducted by a four-point loading test, where the deflection behavior of the beams was monitored through three linear variable displacement transducers (LVDT). The compressive strength and durability properties (i.e., porosity, resistance to chloride ion penetration, and capillary water absorption) were assessed using the same batch of concrete mix used to cast RC beams. The experimental results have shown that the flexural load of RC beams made with IFSSA was significantly higher than the control beam (100% FCBA). The increment of the flexural load was proportional to the content of IFSSA, with an increase of 27% for the beam made with 80% IFSSA than the control beam. The compressive strength of concrete increased by 56% and 61% for the concrete made with 80% and 100% IFSSA, respectively, than the control concrete, which is in good agreement with the flexural load of RC beams. Furthermore, the porosity, resistance to chloride ion penetration, and capillary water absorption were inversely proportional to the increase in the content of IFSSA. For instance, porosity, chloride penetration, and water absorption decreased by 43%, 54%, and 68%, respectively, when IFSSA entirely replaced FCBA. This decreasing percentage of durability properties is in agreement with the flexural load of RC beams. A good linear relationship of porosity with chloride penetration resistance and capillary water absorption was observed.
Strength Characteristics of Alkali-Activated Slag Mortars with the Addition of PET Flakes
Authors: Kocot Agnieszka , Ćwirzeń Andrzej , Ponikiewski Tomasz , Katzer Jacek
Published: October 2021

The production of ordinary Portland cement is associated with significant CO2 emissions. To limit these emissions, new binders are needed that can be efficiently substituted for cement. Alkali-activated slag composites are one such possible binder solution. The research programme presented herein focused on the creation of alkali-activated slag composites with the addition of PET flakes as a partial substitute (5%) for natural aggregate. Such composites have a significantly lower impact in terms of CO2 emissions in comparison to ordinary concrete. The created composites were differentiated by the amount of activator (10 and 20 wt.%) and curing temperature (from 20 to 80 °C). Their mechanical properties were tested, and a scanning electron microscope analysis was conducted. Compressive and flexural strengths ranging from 29.3 to 68.4 MPa and from 3.5 to 6.1 MPa, respectively, were achieved. The mechanical test results confirmed that a higher amount of activator improved the mechanical properties. However, the influence of the PET particles on the mechanical properties and microstructure varied with the curing temperature and amount of activator. Areas that require further research were identified.
Optimization of Cement-Based Mortar Containing Oily Sludge Ash by Response Surface Methodology
Authors: Kankia Mubarak Usman , Baloo Lavania , Danlami Nasiru , Samahani Wan Nurliyana , Mohammed Bashar S. , Haruna Sani , Jagaba Ahmad Hussaini , Abubakar Mahmud , Ishak Effa Affiana , Sayed Khalid , Amila Bt Wan Zawawi Noor
Published: October 2021

In the industries of petroleum extraction, a large volume of oily sludge is being generated. This waste is usually considered difficult to dispose of, causing environmental and economic issues. This study presented the novel experimental method of manufacturing mortar used in civil construction by cement and oily sludge ash (OSA). The defined method was described with a logical experimental study conducted to examine a feasible manufacturing method for casting cement-based mortars by partially replacing cement with OSA. Replacement concentrations for OSA ranged from 0 to 20 percent by cement weight, while the water-to-cement (w/c) ratio was varied from 0.4 to 0.8, and the amount of sand was kept constant. The strengths and absorption rate of the mortar were monitored for 28 days. The OSA contains a crystalline structure with packs of angular grains. Because of OSA in the cement-based mortar mixtures and water-to-cement ratios, the mechanical strength was improved significantly. However, the water absorption trend increased linearly. Using variance analysis, the influence of OSA and w/c ratio on the behavior of mortar was acquired. The developed models were significant for all p-value reactions of <5%. Numerical optimization results showed that the best mixture can be obtained by replacing 8.19 percent cement with OSA and 0.52 as a ratio of w/c.
Preparation and Characterization of Pure SiC Ceramics by HTPVT Induced by Seeding with SiC Nanoarrays
Authors: Deng Yu-Chen , Zhang Nan-Long , Zhi Qiang , Wang Bo , Yang Jian-Feng
Published: October 2021

Dense SiC ceramics were fabricated by high-temperature physical vapor transport (HTPVT) growth process using SiC nanoarrays as the crystal seeds, which was obtained by vacuum heat treatment of amorphous SiC films prepared by plasma-enhanced chemical vapor deposition (PECVD) with a porous anodic aluminum oxide (AAO) template. In the HTPVT process, two-step holding was adopted, and the temperature at the first step was controlled at 2100 and 2150 °C to avoid SiC nanoarrays evaporation, and the grain size of SiC crystal increased with the increase in temperature and decrease in the pressure of Ar. The temperature of the second step was 2300 °C, and rapid SiC grain growth and gradual densification were achieved. The prepared SiC ceramics exhibited a relative density of more than 99%, an average grain size of about 100 μm, a preferred orientation along the (0 0 0 6) plane, a Vickers hardness of about 29 GPa, a flexural strength of about 360 MPa, and thermal conductivity at room temperature of more than 200 W·m−1·K−1.
Fucoidan and Derived Oligo-Fucoses: Structural Features with Relevance in Competitive Inhibition of Gastrointestinal Norovirus Binding
Authors: Hanisch Franz-Georg , Aydogan Cem , Schroten Horst
Published: October 2021

Norovirus infections belong to the most common causes of human gastroenteritis worldwide and epidemic outbreaks are responsible for hundreds of thousands of deaths annually. In humans, noroviruses are known to bind to gastrointestinal epithelia via recognition of blood-group active mucin-type O-glycans. Considering the involvement of l-α-fucose residues in these glycans, their high valency on epithelial surfaces far surpasses the low affinity, though specific interactions of monovalent milk oligosaccharides. Based on these findings, we attempted to identify polyfucoses (fucans) with the capacity to block binding of the currently most prevalent norovirus strain GII.4 (Sydney, 2012, JX459908) to human and animal gastrointestinal mucins. We provide evidence that inhibitory effects on capsid binding are exerted in a competitive manner by α-fucosyl residues on Fucus vesiculosus fucoidan, but also on the galacto-fucan from Undaria pinnatifida and their oligo-fucose processing products. Insight into novel structural aspects of fucoidan and derived oligosaccharides from low-mass Undaria pinnatifida were revealed by GCMS and MALDI mass spectrometry. In targeting noroviral spread attenuation, this study provides first steps towards a prophylactic food additive that is produced from algal species.
Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina
Authors: Xu Yanan , Harvey Patricia J.
Published: October 2021

Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga Dunaliella salina is one of the richest sources of natural carotenoids. We have previously investigated the over-production of phytoene in D. salina after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in D. salina. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support the over-production of phytoene by D. salina cultures as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h of treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in D. salina. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from D. salina.
Can a Set of Questions after Routine Cataract Surgery Predict Unexpected Findings and Avoid an Unnecessary Follow-Up Visit?
Authors: Zemaitiene Reda , Pasiskeviciute Ieva , Varoniukaite Aiste , Pajeda Pijus , Grzybowski Andrzej , Zaliuniene Dalia
Published: October 2021

Background and Objectives: to evaluate whether a set of questions after a routine cataract surgery can predict unexpected findings and avoid an unnecessary follow-up visit. Materials and Methods: single-center, prospective, cohort study included 177 routine cataract surgery cases of two experienced surgeons between November 2019 and December 2020. Inclusion criteria included unremarkable postoperative day one follow-up examination. A set of seven questions regarding complaints with positive or negative answers was presented at the second follow-up visit (PV2)—one week (mean 8.34 ± 1.73 days) after the surgery. The outcome measures were the incidence of unexpected management changes (UMCs) at the PV2 visit (change or addition from a prescribed postoperative drop plan, extra procedures, an urgent referral to an ophthalmologist) and UMCs associations with the answers to a question set. Results: 81.4% of patients had no complaints about postoperative ocular status and answered with negative answers, 18.6% reported one or more complaint (positive answer): dissatisfaction with postoperative visual acuity (6.2%, 11 cases), eye pain (4.0%, 7 cases), increase in floaters after the surgery (4.0%, 7 cases), red eye (4.0%, 7 cases) and others. The prevalence of UMCs at PV2 was 1.7% (3 cases), of which 0.6% (1 case) was the prolonged antibiotic prescription due to conjunctivitis, 0.6% (1 case) was the addition of IOP lowering medication and 0.6% (1 case) was additional medication due to uveitis management. None of the complaints (positive answers) at PV2 were associated with the incidence of UMCs (p > 0.05). Conclusions: there were no associations of UMCs determined with positive answers to the questions. The prediction of UMCs incidence based on the positive answers was not obtained. Thus, we cannot exclude the necessity of a postoperative week one follow-up visit.
Influence of Cross-Wind on CO2 Arc Welding of Carbon Steel
Authors: Tashiro Shinichi , Trinh Ngoc Quang , Suga Tetsuo , Matsuda Natsume , Tsurumaru Naotaka , Maeda Tomohiko , Tanaka Ryohei , Nakatsu Satoshi , Tsujii Gen , Bui Hanh Van , Tanaka Manabu
Published: October 2021

The purpose of this study is to develop a novel welding torch with high wind resistance, which can be used for welding outside a building under strong cross-wind. In this paper, a parametric study was carried out using different torch nozzle designs and shield gas flow rates for their optimization. The gas flow around the torch nozzle exit was visualized through the shadowgraph method to evaluate the interaction between the shielding gas flow and the cross-wind. Nitrogen fraction in a weld bead was measured for confirming the shielding effect. Furthermore, CFD simulation was also carried out for obtaining shielding gas flow velocity at the torch nozzle exit. From the result of the above experiments and simulation, effective parameters for improving the shielding effect against the cross-wind were comprehensively discussed. As a result, the nitrogen fraction was found to be decreased by increasing the averaged vertical gas velocity at the torch nozzle exit. For achieving this, it is especially effective to decrease the nozzle diameter or increase the gas flow rate.
Tree Species and Epiphyte Taxa Determine the “Metabolomic niche” of Canopy Suspended Soils in a Species-Rich Lowland Tropical Rainforest
Authors: Gargallo-Garriga Albert , Sardans Jordi , Alrefaei Abdulwahed Fahad , Klem Karel , Fuchslueger Lucia , Ramírez-Rojas Irene , Donald Julian , Leroy Celine , Langenhove Leandro Van , Verbruggen Erik , Janssens Ivan A. , Urban Otmar , Peñuelas Josep
Published: October 2021

Tropical forests are biodiversity hotspots, but it is not well understood how this diversity is structured and maintained. One hypothesis rests on the generation of a range of metabolic niches, with varied composition, supporting a high species diversity. Characterizing soil metabolomes can reveal fine-scale differences in composition and potentially help explain variation across these habitats. In particular, little is known about canopy soils, which are unique habitats that are likely to be sources of additional biodiversity and biogeochemical cycling in tropical forests. We studied the effects of diverse tree species and epiphytes on soil metabolomic profiles of forest floor and canopy suspended soils in a French Guianese rainforest. We found that the metabolomic profiles of canopy suspended soils were distinct from those of forest floor soils, differing between epiphyte-associated and non-epiphyte suspended soils, and the metabolomic profiles of suspended soils varied with host tree species, regardless of association with epiphyte. Thus, tree species is a key driver of rainforest suspended soil metabolomics. We found greater abundance of metabolites in suspended soils, particularly in groups associated with plants, such as phenolic compounds, and with metabolic pathways related to amino acids, nucleotides, and energy metabolism, due to the greater relative proportion of tree and epiphyte organic material derived from litter and root exudates, indicating a strong legacy of parent biological material. Our study provides evidence for the role of tree and epiphyte species in canopy soil metabolomic composition and in maintaining the high levels of soil metabolome diversity in this tropical rainforest. It is likely that a wide array of canopy microsite-level environmental conditions, which reflect interactions between trees and epiphytes, increase the microscale diversity in suspended soil metabolomes.