bluuebunny/crossref_metadata_2025 huggingface dataset:
Structures and Biological Activities of Secondary Metabolites from Trichoderma harzianum
Authors: Guo Rui , Li Gang , Zhang Zhao , Peng Xiaoping
Published: November 2022

The biocontrol fungus Trichoderma harzianum, from both marine and terrestrial environments, has attracted considerable attention. T. harzianum has a tremendous potential to produce a variety of bioactive secondary metabolites (SMs), which are an important source of new herbicides and antibiotics. This review prioritizes the SMs of T. harzianum from 1988 to June 2022, and their relevant biological activities. Marine-derived SMs, especially terpenoids, polyketides, and macrolides compounds, occupy a significant proportion of natural products from T. harzianum, deserving more of our attention.
Influence of Dispersed TiO2 Nanoparticles via Steric Interaction on the Antifouling Performance of PVDF/TiO2 Composite Membranes
Authors: Zhang Jie , Jian Zicong , Jiang Minmin , Peng Bo , Zhang Yuanyuan , Wu Zhichao , Zheng Junjian
Published: November 2022

Herein, the influence of various contents of polyethylene glycol (PEG) on the dispersion of TiO2 nanoparticles and the comprehensive properties of PVDF/TiO2 composite membranes via the steric hindrance interaction was systematically explored. Hydrophilic PEG was employed as a dispersing surfactant of TiO2 nanoparticles in the pre-dispersion process and as a pore-forming additive in the following membrane preparation process. The slight overlap shown in the TEM image and low TSI value (<1) of the composite casting solution indicated the effective dispersion and stabilization under the steric interaction with a PEG content of 6 wt.%. Properties such as the surface pore size, the development of finger-like structures, permeability, hydrophilicity and Zeta potential were obviously enhanced. The improved antifouling performance between the membrane surface and foulants was corroborated by less negative free energy of adhesion (about −42.87 mJ/m2), a higher interaction energy barrier (0.65 KT) and low flux declination during the filtration process. The high critical flux and low fouling rate both in winter and summer as well as the long-term running operation in A/O-MBR firmly supported the elevated antifouling performance, which implies a promising application in the municipal sewage treatment field.
Water Molecular Dynamics in the Porous Structures of Ultrafiltration/Nanofiltration Asymmetric Cellulose Acetate–Silica Membranes
Authors: Cunha João , da Silva Miguel P. , Beira Maria J. , Corvo Marta C. , Almeida Pedro L. , Sebastião Pedro J. , Figueirinhas João L. , de Pinho Maria Norberta
Published: November 2022

This study presents the characterization of water dynamics in cellulose acetate–silica asymmetric membranes with very different pore structures that are associated with a wide range of selective transport properties of ultrafiltration (UF) and nanofiltration (NF). By combining 1H NMR spectroscopy, diffusometry and relaxometry and considering that the spin–lattice relaxation rate of the studied systems is mainly determined by translational diffusion, individual rotations and rotations mediated by translational displacements, it was possible to assess the influence of the porous matrix’s confinement on the degree of water ordering and dynamics and to correlate this with UF/NF permeation characteristics. In fact, the less permeable membranes, CA/SiO2-22, characterized by smaller pores induce significant orientational order to the water molecules close to/interacting with the membrane matrix’s interface. Conversely, the model fitting analysis of the relaxometry results obtained for the more permeable sets of membranes, CA/SiO2-30 and CA/SiO2-34, did not evidence surface-induced orientational order, which might be explained by the reduced surface-to-volume ratio of the pores and consequent loss of sensitivity to the signal of surface-bound water. Comparing the findings with those of previous studies, it is clear that the fraction of more confined water molecules in the CA/SiO2-22-G20, CA/SiO2-30-G20 and CA/SiO2-34-G20 membranes of 0.83, 0.24 and 0.35, respectively, is in agreement with the obtained diffusion coefficients as well as with the pore sizes and hydraulic permeabilities of 3.5, 38 and 81 kg h−1 m−2 bar−1, respectively, reported in the literature. It was also possible to conclude that the post-treatment of the membranes with Triton X-100 surfactants produced no significant structural changes but increased the hydrophobic character of the surface, leading to higher diffusion coefficients, especially for systems associated with average smaller pore dimensions. Altogether, these findings evidence the potential of combining complementary NMR techniques to indirectly study hydrated asymmetric porous media, assess the influence of drying post-treatments on hybrid CA/SiO2 membrane’ surface characteristics and discriminate between ultra- and nano-filtration membrane systems.
Simulation Analysis and Experimental Study on SLM Forming Titanium Alloy Milling Hole
Authors: Shi Wentian , Yan Tianming , Liu Yude , Zhang Xiaoqing , Li Jihang , Wang Lin , Dong Lu
Published: November 2022

Using finite element analysis software and based on the Johnson–Cook failure criterion, a 3D printing workspace model with collapse, powder sticking, and cavity defects was established under the selective laser melting (SLM) forming process. The simulation analysis of milling holes was carried out, and the relationship between cutting speed and material-removal rate on tool wear and entrance burr was derived. The hole-milling experiment was carried out to verify the dimensional accuracy and surface appearance of the hole under the two processes of SLM direct forming and re-milling after forming; the inhibition effect of re-milling after forming on collapse, powder sticking, and cavity defects in hole forming were studied, and the formation mechanism of various defects such as burrs, scratches, and hole-wall cracks in the hole-milling process was analyzed. The Kistler9129A dynamometer was used to measure the cutting forces of re-milling holes and direct milling holes, and a comparative analysis was carried out. The influence of cutting speed, hole diameter, and material-removal rate on the axial force of milling holes was explored. The experiment results were consistent with the simulation cutting model, and the model’s accuracy was verified.
Corrosion Study of 80S Steel under the Coexistence of CO2 and H2S
Authors: Song Pu , Wang Wenzhen , Jia Xingang
Published: November 2022

The continuous rise in the energy demand has shifted the extraction environment in oil and gas fields towards a more hostile environment, and has ultimately increased the corrosion of extraction and transmission facilities. One of the most effective solutions for mitigating the corrosion problem is the use of corrosion-resistant metals. In this paper, we investigated the corrosion behavior of 80S steel that was being employed in an oilfield underground gathering pipeline at different temperatures and partial pressures of H2S and CO2 using an autoclave. Moreover, the loss-in-weight method was used to simulate the corrosive environment in the oilfield. Electrochemical studies were then carried out to investigate the corrosion mechanism. The results show that: (1) In the corrosive environment of CO2 and H2S coexistence, temperature is a major factor affecting the corrosion rate of 80S steel, and increase in temperature accelerates the corrosion process. (2) Corrosion rate is also affected by the CO2 and H2S partial pressure ratio; high S content at high temperatures will inhibit the corrosion process, and vice versa for low temperature. (3) With an increase in the temperature, the corrosion potential decreases, corrosion current density increases, and polarization curve gradually moves to the right. (4) The shape of the cathodic branch moves in the X-negative direction by increasing S content, and the cathodic reaction is jointly controlled by activation and diffusion processes, when the temperature is 100 °C, whereas the anodic branch of the polarization curve at a 3% concentration of Na2S.9H2O changes significantly and a passivation zone appears. (5) The results of the impedance spectra showed that the impedance radius of the metal decreases significantly at increasing temperatures. In addition, the Warburg impedance showed a more pronounced diffusion phenomenon with the increasement of H2S concentration.
Non-Targeted Serum Lipidomics Analysis and Potential Biomarkers of Laryngeal Cancer Based on UHPLC-QTOF-MS
Authors: Wang Haoyue , Luo Yanbo , Chen Huan , Hou Hongwei , Hu Qingyuan , Ji Min
Published: November 2022

Laryngeal cancer is a common head and neck malignant cancer type. However, effective biomarkers for diagnosis are lacking and pathogenesis is unclear. Lipidomics is a powerful tool for identifying biomarkers and explaining disease mechanisms. Hence, in this study, non-targeted lipidomics based on ultra-performance liquid chromatography–quadrupole time of flight–mass spectrometry (UHPLC-QTOF-MS) were applied to screen the differential lipid metabolites in serum and allowed for exploration of the remodeled lipid metabolism of laryngeal cancer, laryngeal benign tumor patients, and healthy crowds. Multivariate analysis and univariate analysis were combined to screen for differential lipid metabolites among the three groups. The results showed that, across a total of 57 lipid metabolic markers that were screened, the regulation of the lipid metabolism network occurred mainly in phosphatidylcholine (PC), lysophosphatidylcholine (LPC), and sphingomyelin (SM) metabolism. Of note, the concentration levels of sphingolipids 42:2 (SM 42:2) and sphingolipids 42:3 (SM 42:3) correlated with laryngeal cancer progression and were both significantly different among the three groups. Both of them could be considered as potential biomarkers for diagnosis and indicators for monitoring the progression of laryngeal cancer. From the perspective of lipidomics, this study not only revealed the regulatory changes in the lipid metabolism network, but also provided a new possibility for screening biomarkers in laryngeal cancer.
Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)
Authors: Li Xuehe , Yi Yuanming , Wu Jiahua , Yang Qihui , Tan Beiping , Chi Shuyan
Published: November 2022

In a context where the search for plant-derived additives is a hot topic, glycerol monolaurate (GML) was chosen as our subject to study its effect on grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). Seven gradient levels of GML (0, 600, 1200, 1800, 2400, 3000, and 3600 mg/kg) were used for the experiment. Based on our experiments, 1800 mg/kg GML significantly increased the final body weight (FBW) and weight gain rate (WGR). GML increased the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreased malondialdehyde (MDA). Adding 1800 mg/kg GML also significantly increased the levels of lauric acid (C12:0) (LA), n-3 polyunsaturated fatty acids (PFA), and the n-6 PFA-to-n-3/n-6 ratio, while significantly decreasing the levels of saturated fatty acids (SFA). Dietary supplementation with GML significantly inhibited the expression of pro-inflammatory factors and reduced the occurrence of inflammation. GML improved intestinal flora and the abundance of beneficial bacteria (Bacillus, Psychrobacter, Acinetobacter, Acinetobacter, Stenotrophomonas, and Glutamicibacter). It provides a theoretical basis for the application of GML in aquafeed and greatly enhances the possibility of using GML in aquafeed. Based on the above experimental results, the optimum level of GML in grouper feed is 1800 mg/kg.
The Influence of Energy Balance, Lipolysis and Ketogenesis on Metabolic Adaptation in Cows Milked Twice and Three Times Daily
Authors: Krnjaić Srđan , Cincović Marko , Djoković Radojica , Belić Branislava , Ježek Jožica , Starič Jože
Published: November 2022

Increasing milk production requires increasing milking frequency (MF) from two times daily (2X) to three (3X) or more. High milk production leads to negative energy balance (NEB) and homeorhesis, characterized by lipolysis, ketogenesis, and endocrinological changes. The relationship among energy balance (EB), lipolysis, and ketogenesis with endocrine and metabolic parameters in blood of cows milked 2X and 3X daily was studied. Holstein Friesian cows milked 2X (n = 45) and 3X (n = 45) were analyzed, with approximately 50% of cows in each group in positive EB (PEB) and 50% in NEB. After determining EB, blood samples were collected from all cows and blood serum was analyzed for non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), glucose (GLU), cholesterol (CHOL), triglycerides (TGC), total bilirubin (TBIL), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), calcium (Ca), inorganic phosphate (P), total protein (TPROT), albumin (ALB), urea, insulin (INS), T3, T4, and cortisol (CORT), and the RQUICKIBHB index of insulin resistance was calculated. Cows milked 3X in NEB represent a special cluster that partially overlaps with cows milked 2X in NEB and has no contact points with cows in PEB. Cows milked 3X had higher levels of NEFA, BHB, AST, GGT, TBIL, and CORT and lower levels of GLU, Ca, INS, and T4. Cows milked 3X in NEB had higher levels of NEFA, BHB, AST, GGT, TBIL, and CORT and lower levels of GLU, CHOL, TGC, TPROT, P, INS, RQUICKIBHB, and T3 compared with cows milked 2X in NEB and cows in PEB. In cows milked 3X, lipolysis and ketogenesis were much more prominent, and EB levels were lower, implying a pronounced shift in homeorhesis. Metabolic and endocrinology parameters were determined mainly by the values of EB, NEFA, and BHB in cows milked 3X in NEB compared with other categories of cows. The results confirm the peculiarity of metabolic adaptation in cows with increased MF, characterized not only by differences in the concentration of metabolites but also in their interactions.
Correlation of Superlattice Cross-Plane Thermal Conductivity with Emission Wavelength in InAlAs/InGaAs Quantum Cascade Lasers
Authors: Villalobos Meza Alejandro M. , Shahzad Monas , Hathaway Dagan , Shu Hong , Lyakh Arkadiy
Published: November 2022

The low cross-plane thermal conductivity of quantum cascade lasers (QCLs) is a significant limitation in their Continuous-Wave (CW) performance. Structural parameters such as individual layer thicknesses and interface density vary for QCLs with different target emission wavelengths, and these design parameters are expected to influence the cross-plane thermal conductivity. Though previous works have used theoretical models and experimental data to quantify thermal conductivity, the correlation between target wavelength and thermal conductivity has yet to be reported for QCLs. In this work, we observe a general trend across a group of QCLs emitting from 3.7 to 8.7 µm: as the QCL design changes to reduce wavelength, the thermal conductivity decreases as well. Numerically, we measured an approximate 70% reduction in thermal conductivity, from 1.5 W/(m·K) for the 8.7 µm device, to 0.9 W/(m·K) for the 3.7 µm device. Analysis of these structures with the Diffuse Mismatch Model (DMM) for thermal boundary resistance (TBR) shows that the largest contribution of this effect is the impact of superlattice interface density on the thermal conductivity. The observed changes in conductivity result in significant changes in projected CW optical power and should be considered in laser design.
Two-Degree-of-Freedom Piezoelectric Energy Harvesting from Vortex-Induced Vibration
Authors: Lu De , Li Zhiqing , Hu Guobiao , Zhou Bo , Yang Yaowen , Zhang Guiyong
Published: November 2022

In recent years, vortex-induced vibration (VIV) has been widely employed to collect small-scale wind energy as a renewable energy source for microelectronics and wireless sensors. In this paper, a two-degree-of-freedom (2DOF) VIV-based piezoelectric energy harvester (VIVPEH) was designed, and its aerodynamic characteristics were thoroughly investigated. First, based on the traditional model theory and combined with the knowledge of vibration dynamics, the governing equations of the 2DOF VIVPEH were established. The dynamic responses, including the displacement and voltage output, were numerically simulated. Compared with the traditional 1DOF VIVPEH, the 2DOF VIVPEH proposed in this paper produced two lock-in regions for broadband wind energy harvesting. Furthermore, it was unveiled that the first- and second-order resonances were induced in the first and lock-in regions, respectively. Subsequently, a parametric study was conducted to investigate the influences of the circuit and mechanical parameters on the energy harvesting performance of the 2DOF VIVPEH. It was found that when the 2DOF VIVPEH was induced to vibrate in different lock-in regions, its optimal resistance became different. Moreover, by varying the masses and stiffnesses of the primary and secondary DOFs, we could adjust the lock-in regions in terms of their bandwidths, locations, and amplitudes, which provides a possibility for further customization and optimization.
Threshold Switching in Forming-Free Anodic Memristors Grown on Hf–Nb Combinatorial Thin-Film Alloys
Authors: Zrinski Ivana , Zavašnik Janez , Duchoslav Jiri , Hassel Achim Walter , Mardare Andrei Ionut
Published: November 2022

The development of novel materials with coexisting volatile threshold and non-volatile memristive switching is crucial for neuromorphic applications. Hence, the aim of this work was to investigate the memristive properties of oxides in a Hf–Nb thin-film combinatorial system deposited by sputtering on Si substrates. The active layer was grown anodically on each Hf–Nb alloy from the library, whereas Pt electrodes were deposited as the top electrodes. The devices grown on Hf-45 at.% Nb alloys showed improved memristive performances reaching resistive state ratios up to a few orders of magnitude and achieving multi-level switching behavior while consuming low power in comparison with memristors grown on pure metals. The coexistence of threshold and resistive switching is dependent upon the current compliance regime applied during memristive studies. Such behaviors were explained by the structure of the mixed oxides investigated by TEM and XPS. The mixed oxides, with HfO2 crystallites embedded in quasi amorphous and stoichiometrically non-uniform Nb oxide regions, were found to be favorable for the formation of conductive filaments as a necessary step toward memristive behavior. Finally, metal–insulator–metal structures grown on the respective alloys can be considered as relevant candidates for the future fabrication of anodic high-density in-memory computing systems for neuromorphic applications.
Formation and Penetration Properties of a Shaped Charge with Zr41.2Ti13.8Cu12.5Ni10Be22.5 Liner
Authors: Zu Xudong , Chen Taian , Tan Yaping , Chen Hao , Huang Zhengxiang
Published: November 2022

In the military field, determining how to increase the hole-expanding ability of shaped charge warheads is a key and difficult issue with respect to warhead development. Amorphous alloys have grains or grain boundaries, with unique mechanical properties. Zr41.2Ti13.8Cu12.5Ni10Be22.5 can be used as the liner material of shaped charges, resulting in high-speed particle flows that differ from those of traditionally shaped charges. In this paper, based on the analysis of the mechanical response characteristics of Zr41.2Ti13.8Cu12.5Ni10Be22.5 and its fracture morphology under impact, combined with the formation theory of shaped charge jets, a semi-empirical formula is derived to calculate the velocity of non-cohesive high-speed particle flow considering the elastic strain energy loss. Additionally, the reliability of the proposed theoretical model is verified through experiments. The penetration process of Zr-based amorphous alloy high-speed particle flow into a concrete target is theoretically analyzed, and the penetration stages of the high-speed particle flow into the target are clearly distinguished. Combined with the penetration theory of shaped charge particle jets, a high-speed particle flow penetration model is proposed, and a pore expansion model is established through an energy method. The experimentally obtained data on depth of penetration are in agreement with the theoretical calculation results.
Prospects of Probiotic Adjuvant Drugs in Clinical Treatment
Authors: Ren Zhongyue , Hong Yan , Huo Yalan , Peng Lingling , Lv Huihui , Chen Jiahui , Wu Zhihua , Wan Cuixiang
Published: November 2022

In modern society, where new diseases and viruses are constantly emerging, drugs are still the most important means of resistance. However, adverse effects and diminished efficacy remain the leading cause of treatment failure and a major determinant of impaired health-related quality of life for patients. Clinical studies have shown that the disturbance of the gut microbial structure plays a crucial role in the toxic and side effects of drugs. It is well known that probiotics have the ability to maintain the balance of intestinal microecology, which implies their potential as an adjunct to prevent and alleviate the adverse reactions of drugs and to make medicines play a better role. In addition, in the past decade, probiotics have been found to have excellent prevention and alleviation effects in drug toxicity side effects, such as liver injury. In this review, we summarize the development history of probiotics, discuss the impact on drug side effects of probiotics, and propose the underlying mechanisms. Probiotics will be a new star in the world of complementary medicine.
Improving Early Optics Instruction Using a Phenomenological Approach: A Field Study
Authors: Fliegauf Kai , Sebald Janika , Veith Joaquin Marc , Spiecker Henrike , Bitzenbauer Philipp
Published: November 2022

Previous research has shown that phenomenological approaches in early optics education might be superior to traditional model-based instruction based on the light ray realm with regards to fostering students’ conceptual understanding of basic optics topics. However, it remains open to date which learning difficulties students encounter when being introduced to optics following a phenomenological approach—in particular, in comparison to the learning difficulties that are widespread among students introduced to optics via traditional model-based instruction. With this article, we contribute to closing this gap: We report the results of a quasi-experimental field study with N=189 secondary school students. We used ten items adapted from the literature in a pre-posttest design for an in-depth exploration of the conceptions of introductory optics topics acquired by N=89 students introduced to optics following a phenomenological teaching-learning sequence and compare these students’ conceptions to the ones acquired by N=100 peers who participated in traditional model-based instruction covering the same content topics. The results of this study substantiate earlier findings according to which phenomenological teaching might be a fruitful endeavour for early optics education, in particular, when it comes to teaching and learning about image formation by converging lenses.
A Seroepidemiological Survey of Corynebacterium pseudotuberculosis Infection in South Tyrol, Italy
Authors: Bettini Astrid , Mancin Marzia , Mazzucato Matteo , Schanung Angelica , Colorio Stefano , Tavella Alexander
Published: November 2022

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a bacterial infection that can affect livestock. This infection can cause low growth rates and milk yields and reproductive failure, along with the infection of humans, especially those in close contact with the animals. The aim of this study was to evaluate the local CLA prevalence, highlighting the parameters for the potential predisposition to infection in goats, and to implement a monitoring program based on the newly acquired scientific evidence. Of a total of 2365 goat farms in South Tyrol, 384 farms were selected for the present study. A statistically significant number of animals were subjected to serologic analysis for the detection of C. pseutotubercolosis antibodies. A total of 2948 goats belonging to the selected farms were tested for CLA, 713 of which showed a positive result. The data analysis led to an estimated CLA seroprevalence of 21.85%. The results achieved can enable the evaluation and possible implementation of a voluntary program that permits us to test a larger number of animals using serological techniques. This program would be of great importance, especially for those farms dedicated to the production of milk and dairy products, as some manufacturing practices may increase the risk of transmission of zoonotic pathogens such as C. pseudotuberculosis to humans.
Unusual Localization of Hysterothylacium Incurvum in Xiphias gladius (Linnaeus 1758) Caught in the Atlantic Ocean
Authors: De Benedetto Giovanni , Corti Ivan , Malandra Renato , Riolo Kristian , Giannetto Alessia , Gaglio Gabriella
Published: November 2022

This study represents the first report of Hysterothylacium incurvum within swordfish (Xiphias gladius) heart chambers. Swordfish is a large pelagic teleost, considered one of the most appreciated fish worldwide. Among swordfish parasites, Anisakis sp. and Hysterothylacium sp. have been used to evaluate biological and ecological aspects of this teleost. Between 2021 and 2022, 364 X. gladius hearts, caught from the Atlantic Ocean (FAO 27.IXa and FAO 34 areas), were collected at the Milan fish market (Lombardy, Italy). Three specimens from FAO 27.IXa was positive for seven adult nematodes (p = 1.55%) within the heart chambers. Of these, three specimens were found within the bulbus arteriosus and 4 in the ventricle. All parasites were stored in 70% ethanol and processed for parasitological and molecular analysis using Cox2, ITS regions/ITS-I-5.8S-ITS-II, and rrnS genes. The analysis allowed us to identify the retrieved parasite as H. incurvum. According to our evaluation, the final localization is due to the movement of L3 larvae from the coelomic cavity to the bloodstream, with consequent development to the adult stage within the heart. Finally, the parasite localization, considered non-marketable fish parts, does not pose a significant risk to consumers, also considering the low zoonotic potential of H. incurvum.
The Effect of Pegbovigrastim Injection on Phagocytic and Oxidative Burst Activities of Peripheral Blood Granulocytes and Monocytes in Calves Challenged with Mycoplasma bovis
Authors: Dudek Katarzyna , Szacawa Ewelina , Wasiak Magdalena , Bednarek Dariusz , Reichert Michał
Published: November 2022

Mycoplasma bovis (M. bovis) is an important pathogen affecting cattle, causing various diseases including pneumonia which mainly occurring in calves. Control of M. bovis infections is difficult due to the lack of commercial vaccines in most parts of the world and increasing trends of antimicrobial resistance in field isolates of the pathogen; therefore, it seems reasonable to look for new solutions for the prevention of the infection. Pegbovigrastim is a pegylated form of naturally occurring circulating cytokine in cattle that affects bovine leukocytes and some cell functions. Most studies on pegbovigrastim have focused on reducing the occurrence of mastitis and other diseases occurring during the periparturient period in cows, while this study attempts to use pegbovigrastim in the prevention of respiratory diseases in calves, which are largely caused by M. bovis. Based on previous observations on the immunostimulatory properties of pegbovigrastim in cattle, for the first time, the effect of its injection on the number and phagocytic and oxidative burst activities of peripheral blood granulocytes and monocytes in calves experimentally infected with M. bovis was investigated. Pegbovigrastim administration in the calves significantly stimulated an increase in peripheral blood granulocyte and monocyte counts and phagocytic activity of the cells, especially granulocytes, which was also generally expressed in the course of M. bovis infection. In response to pegbovigrastim administration, a general increase in the oxygen burst activity of the cells was observed. This effect was also shown despite ongoing infection with M. bovis which, taken together, may indicate a beneficial effect of pegbovigrastim injection on the immunity of the affected animals.
Association between Thyroid Function and Respiratory Distress Syndrome in Preterm Infants
Authors: Kim Yonghyuk , Kim Youngjin , Chang Meayoung , Lee Byoungkook
Published: November 2022

Thyroid hormones are known to influence the production and secretion of pulmonary surfactant. The objective of this study was to explore the relationship between respiratory distress syndrome (RDS) and thyroid hormones. This was a retrospective study of preterm infants at 24–33 weeks gestational age from April 2017 to February 2019. T3, free T4 (fT4), and thyroid-stimulating hormone (TSH) were measured 1, 3, and 6 weeks after birth. Multivariate logistic regression analyses were performed to determine the relationship between RDS and TSH. A total of 146 infants were enrolled. Of these, 60 had RDS, 72 had no RDS, and 14 were excluded. T3 and TSH were lower in the RDS groups (p < 0.05) on the day of birth. Multivariate logistic regression analysis indicated that lower serum TSH levels immediately after birth were associated with a higher incidence of RDS (OR, 0.89; 95% CI, 0.81–0.97). The TSH level was associated with the incidence of RDS. This suggests that suppression of the hypothalamus–pituitary axis function contributes to RDS, which is the result of surfactant deficiency.
Easy Synthesis and Characterization of Novel Carbon Dots Using the One-Pot Green Method for Cancer Therapy
Authors: Wang Lijie , Gu Donghao , Su Yupei , Ji Dongxu , Yang Yue , Chen Kai , Pan Hao , Pan Weisan
Published: November 2022

In this study, hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) were used for the synthesis of novel targeted nanocarrier carbon dots (CDC-H) with photo-luminescence using a one-step hydrothermal method. Doxorubicin (DOX), a common chemotherapeutic agent, was loaded with the CDC-H through electrostatic interactions to form DOX–CDC-H complexes as a targeted antitumor drug delivery system. The synthesized CDC-H show a particle size of approximately 6 nm and a high fluorescence quantum yield of 11.64%. The physical and chemical character properties of CDC-H and DOX–CDC-H complexes were investigated using various techniques. The results show that CDC-H have stable luminescent properties and exhibit excellent water solubility. The in vitro release study showed that DOX–CDC-H exhibited pH-dependent release for 24 h. Confocal laser scanning microscopy was applied to investigate the potential of CDC-H for cell imaging and the cellular uptake of DOX–CDC-H in different cells (NIH-3T3 and 4T1 cells), and the results confirmed the target cell imaging and cellular uptake of DOX–CDC-H by specifically binding the CD44 receptors on the surface of tumor cells. The r MTT results suggest that the DOX–CDC-H complex may induce apoptosis in 4T1 cells, reducing the cytotoxicity of free DOX-induced apoptosis. In vivo antitumor experiments of DOX–CDC-H exhibited enhanced tumor cancer therapy. CDC-H have potential applications in bioimaging and antitumor drug delivery.
Coffea canephora: Heterotic Crosses Indicated by Molecular Approach
Authors: Sousa Priscila , Vieira Henrique , Santos Eileen , Viana Alexandre , Boaechat Marcela , Partelli Fábio
Published: November 2022

The genus Coffea comprises more than 100 species, of which the most commercially important are Coffea arabica and Coffea canephora. The latter is a self-incompatible plant with high natural genetic variability. The detection of polymorphism at the DNA level by molecular markers allowed significant progress with the selection of superior plants. The objective of this study was the molecular characterization of C. canephora using microsatellite markers. To this end, a population of forty-four C. canephora genotypes and one C. arabica genotype, was evaluated with 21 primers. These primers identified 61 alleles in the population and between 2 and 5 alleles per locus. The information index indicated a high level of polymorphism of the analyzed markers. According to the observed and expected heterozygosity, the genetic diversity in the population is high. The overall inbreeding coefficient of the population detected high heterozygosity and zero inbreeding within this population. Genetic diversity among the accessions was also evaluated by the unweighted pair-group method based on arithmetic averages (UPGMA). Six groups were formed based on Mojena’s cutting rule and three using the Bayesian approach. These results confirmed the existence of genetic diversity, genetic variability and a potential for selection in future breeding efforts involving the 45 genotypes studied.
Phytomanagement of a Lead-Polluted Shooting Range Using an Aromatic Plant Species and Its Effects on the Rhizosphere Bacterial Diversity and Essential Oil Production
Authors: Saran Anabel , Fernandez Lucia , Latini Cinthia Yanela , Reinhard Monica Bellozas , Minig Marisol , Thijs Sofie , Vangronsveld Jaco , Merini Luciano Jose
Published: November 2022

This field study aimed to assess the baseline conditions of a long-term shooting range in Argentina polluted with 428 mg kg−1 lead (Pb) to evaluate the establishment and development of Helianthus petiolaris plants and address the efficacy of the phytomanagement strategy through: (i) element accumulation in plant tissues; (ii) rhizosphere bacterial diversity changes by Illumina Miseq™, and (iii) floral water and essential oil yield, composition, and element concentration by GC–MS and ICP. After one life cycle growing in the polluted sites, in the roots of Helianthus petiolaris plants, Pb concentration was between 195 and 304 mg kg−1 Pb. Only a limited fraction of the Pb was translocated to the aerial parts. The predominance of the genus Serratia in the rhizosphere of Helianthus petiolaris plants cultivated in the polluted sites and the decrease in the essential oil yield were some effects significantly associated with soil Pb concentration. No detectable Pb concentration was found in the floral water and essential oil obtained. Extractable Pb concentration in the soil reduced between 28% and 45% after the harvest.
Ecophysiological Response of Vitis vinifera L. in an Urban Agrosystem: Preliminary Assessment of Genetic Variability
Authors: Brunori Elena , Bernardini Alessandra , Moresi Federico Valerio , Attorre Fabio , Biasi Rita
Published: November 2022

Urban agriculture is an emerging challenge. Identifying suitable agrosystems that allow for the multiple functions of urban agriculture represents a key issue for the reinforcement of the agricultural matrix in cities, with the aims of counteracting and adapting to climate change and providing economic and social benefits. This study aims to produce a preliminary assessment of the adaptability of Italian native and non-native Vitis vinifera L. cultivars to the stressors of an urban environment. The investigation was carried out on the grapevine collection of the Botanical Garden of Rome (“Vigneto Italia”). A total of 15 grapevine varieties were selected for the evaluation of leaf chlorophyll content, stomatal conductance, and chlorophyll fluorescence under abiotic conditions during the growing season of 2021. Spectral signatures were collected from mature leaves, and several vegetation indices (LWI, MCARI, and WBI) were calculated. Our preliminary results highlighted differences in the behavior of the grapevine cultivars. The native ones showed a medium-high level for leaf chlorophyll content (greater than 350 mol m−2), good photosynthetic efficiency (QY > 0.75), and optimal stomatal behavior under drought stress (200 > gs > 50 mmol H2O m−2 s−1). The data allowed for the classification of the tested genotypes based on their site-specific resistance and resilience to urban environmental conditions. The grapevine proved to be a biological system that is highly sensitive to climate variables, yet highly adaptable to limiting growing factors.
Vacuum Arc Plasma Coating for Polymer Surface Protection— A Plasma Enhanced In-Orbit Additive Manufacturing Concept
Authors: Kühn-Kauffeldt Marina , Kühn Marvin , Mallon Michael , Saur Wolfgang , Fuchs Fabian
Published: November 2022

In-orbit additive manufacturing (AM) is a promising approach for fabrication of large structures. It allows to expand and accelerate human space exploration possibilities. Extrusion-based AM was demonstrated in zero gravity, while the realization of such a process in orbit-like vacuum conditions is currently under exploration. Still, a solution for protection of the UV and IR radiation sensitive polymers is needed in order to prevent their early mechanical failure under space conditions. Vacuum arc plasma based process is widely applied on earth for thin protective coating deposition. Its major advantage is its scalability—from tiny size used in electric propulsion to large scale coating devices. The usability of the vacuum arc process in space conditions was shown in electric propulsion applications in nano-satellites. In this work we discuss and demonstrate the integration of vacuum arc process as a post processing step after Fused Filament Fabrication (FFF) for additive manufacturing and functionalization of long polymer structures. Here we address the concept for technical realization, which integrates the vacuum arc into additive manufacturing process chain. More over we present a laboratory prototype, which implements this concept together with a use case, where a previously printed PEEK structure is coated with aluminum based coating suitable for UV radiation protection.
Introducing Platforms: A Transdisciplinary Journal on Platform Management, Services and Policy and All Related Research
Authors: Ben Youssef Adel
Published: November 2022

The rise of the platform is one of the three iconic events of the “digital revolution” [...]
Experimental and Numerical Investigation of Joints for a Pultruded Fiber-Reinforced Polymer Truss
Authors: Chen Yiwei , Duan Maojun , Zou Xingxing , Feng Yu , Li Guofen
Published: November 2022

Bolted connections usually govern the structural rigidity and load-carrying capacity of pultruded glass fiber-reinforced polymer (GFRP) truss structures. In this study, a novel bolted integrated gusset plate (IGP) connection is proposed to enhance the stiffness and capacity of GFRP truss structures. Nine double-lap shear tests of GFRP joints and numerical simulation were conducted to investigate the influence of variable design parameters of the bolted GFRP joints (number of bolts, width and thickness of GFRP, edge distance of bolts, and the employment of adhesive). Three full-scale GFRP truss joints were tested under static loading to study the response of a typical bolted connection, a bolted gusset plate connection, and the proposed IGP connection. The nine double-lap shear tests showed that the bolted–bonded mixed connection has 50% higher shear stiffness and 27% higher ductility compared with bolted joints, and bearing failure dominated the capacity of most specimens, which agreed well with numerical simulation results. Tests on the three full-scale GFRP truss joints showed that the bolted gusset plate can substantially reduce the number of cracks and improve the initial stiffness, but the maximum bearing capacity of the joints did not increase because the shear fracture of pultruded GFRP webs governs the capacity. The proposed IGP substantially increased the stiffness and capacity compared with the bolted connection and typical bolted gusset plate connection. The full-scale GFRP joint test is suggested to be used together with direct shear tests to study the performance of joints of the GFRP truss.
Acceleration of Bone Fracture Healing through the Use of Bovine Hydroxyapatite or Calcium Lactate Oral and Implant Bovine Hydroxyapatite–Gelatin on Bone Defect Animal Model
Authors: Budiatin Aniek Setiya , Khotib Junaidi , Samirah Samirah , Ardianto Chrismawan , Gani Maria Apriliani , Putri Bulan Rhea Kaulika Hadinar , Arofik Huzaifah , Sadiwa Rizka Nanda , Lestari Indri , Pratama Yusuf Alif , Rahadiansyah Erreza , Susilo Imam
Published: November 2022

Bone grafts a commonly used therapeutic technique for the reconstruction and facilitation of bone regeneration due to fractures. BHA–GEL (bovine hydroxyapatite–gelatin) pellet implants have been shown to be able accelerate the process of bone repair by looking at the percentage of new bone, and the contact between the composite and bone. Based on these results, a study was conducted by placing BHA–GEL (9:1) pellet implants in rabbit femoral bone defects, accompanied by 500 mg oral supplement of BHA or calcium lactate to determine the effectiveness of addition supplements. The research model used was a burr hole defect model with a diameter of 4.2 mm in the cortical part of the rabbit femur. On the 7th, 14th and 28th days after treatment, a total of 48 New Zealand rabbits were divided into four groups, namely defect (control), implant, implant + oral BHA, and implant + oral calcium lactate. Animal tests were terminated and evaluated based on X-ray radiology results, Hematoxylin-Eosin staining, vascular endothelial growth Factor (VEGF), osteocalcin, and enzyme-linked immunosorbent assay (ELISA) for bone alkaline phosphatase (BALP) and calcium levels. From this research can be concluded that Oral BHA supplementation with BHA–GEL pellet implants showed faster healing of bone defects compared to oral calcium lactate with BHA–GEL pellet implants.
Novel In Situ Modification for Thermoplastic Starch Preparation based on Arenga pinnata Palm Starch
Authors: Ghozali Muhammad , Meliana Yenny , Chalid Mochamad
Published: November 2022

Thermoplastic starch (TPS) has three main disadvantages, i.e., poor mechanical properties, low thermal stability and water sensibility. To overcome these disadvantages, TPS properties can be improved by starch modification, adding reinforcements and blending with other polymers. In this research, to prepare modified TPS, starch modification was carried out by in situ modification. The modified TPS was prepared by adding Arenga pinnata palm starch (APPS), glycerol and benzoyl peroxide simultaneously in the twin-screw extruder. Morphology analysis of TPS revealed that the starch granules were damaged and gelatinized in the extrusion process. No phase separation is observed in TPS, which exhibits that starch granules with and without benzoyl peroxide were uniformly dispersed in the matrix. The addition of benzoyl peroxide resulted in increased density of TPS from 1.37 to 1.39 g·cm−3, tensile strength from 7.19 to 8.61 MPa and viscosity from 2482.19 to 2604.60 Pa.s. However, it decreased the elongation at break of TPS from 33.95 to 30.16%, melt flow rate from 7.13 to 5.73 gr/10 min and glass transition temperature from 65 to 52 °C. In addition, the thermal analysis showed that the addition of benzoyl peroxide increased the thermal stability of TPS and extended the temperature range of thermal degradation.
Activated Carbon Aerogel as an Electrode with High Specific Capacitance for Capacitive Deionization
Authors: Wang Wei , Li Kerui , Song Ge , Zhou Minghua , Tan Peng
Published: November 2022

In this study, carbon aerogels (CAs) were synthesized by the sol-gel method, using environmentally friendly glucose as a precursor, and then they were further activated with potassium hydroxide (KOH) to obtain activated carbon aerogels (ACAs). After the activation, the electrochemical performance of the ACAs was significantly improved, and the specific capacitance increased from 19.70 F·g−1 to 111.89 F·g−1. Moreover, the ACAs showed a stronger hydrophilicity with the contact angle of 118.54° compared with CAs (69.31°). When used as an electrode for capacitive deionization (CDI), the ACAs had not only a better diffuse electric double layer behavior, but also a lower charge transfer resistance and intrinsic resistance. Thus, the ACA electrode had a faster CDI desalination rate and a higher desalination capacity. The unit adsorption capacity is three times larger than that of the CA electrode. In the desalination experiment of 100 mg·L−1 sodium chloride (NaCl) solution using a CDI device based on the ACA electrode, the optimal electrode spacing was 2 mm, the voltage was 1.4 V, and the flow rate was 30 mL·min−1. When the NaCl concentration was 500 mg·L−1, the unit adsorption capacity of the ACA electrode reached 26.12 mg·g−1, much higher than that which has been reported in many literatures. The desalination process followed the Langmuir model, and the electro-sorption of the NaCl was a single layer adsorption process. In addition, the ACA electrode exhibited a good regeneration performance and cycle stability.
Assessing a Revised Compensation Theodicy
Authors: Reichenbach Bruce R.
Published: November 2022

Attempts to resolve the problem of evil often appeal to a greater good, according to which God’s permission of moral and natural evil is justified because (and just in case) the evil that is permitted is necessary for the realization of some greater good. In the extensive litany of greater good theodicies and defenses, the appeal to the greater good of an afterlife of infinite reward or pleasure has played a minor role in Christian thought but a more important role in Islamic thought. In a recent article, Seyyed Jaaber Mousavirad invites us to reconsider the greater good theodicy of compensation. He contends that not only are all evils justified in that God compensates the sufferer in an afterlife, but because the evils experienced produce some good, God has reason for bringing about or allowing evils in the first place. In what follows, I argue that this modified compensation theodicy is flawed in its premises, faces serious problems with its concept of justice, treats people as means only and not as intrinsically valuable, and ultimately fails to show that an afterlife compensation, along with some good produced here and now by evil, justify God bringing about or allowing evil.
“Interpreting Buddhist Precepts with Confucian Rites” Based on Their Similarity and Dissimilarity: A Perspective of the History of Ideas in Wei, Jin, and Northern and Southern Dynasties
Authors: Sheng Kai , Zhou Bangwei
Published: November 2022

The “similarity” (gongtong 共通) and “dissimilarity” (chayi 差異) between the Buddhist precepts and Confucian rites in the Wei, Jin, and Southern and Northern Dynasties reflected a “dialogue of civilizations” (wenming duihua 文明對話) at the levels of concept, system, and life. During these periods, the Chinese system of rites were compared and interpreted with the Buddhist monastic codes by Buddhist monks and Confucian scholars, so a history of the ideas interpretation process of “interpreting precepts with rites” (yi li shi jie 以禮釋戒) was achieved. The result of such a process was two-fold: from the perspective of lay Buddhist ethics, they were in common with each other; from the perspective of monastic precepts, they were irreconcilable contradictions. Thus, on the one hand, the eminent Chinese monks “were emulating the Confucian rites to justify Buddhist precepts” (ni li yi zheng jie 擬禮義證戒) to stress their commonalities. On the other hand, the differences between the precepts and rites continued to be discovered, and the Buddhist subjective consciousness (zhuti yishi 主體意識) of “the distinction between precepts and rites” (jie li you bie 戒禮有別) was gradually established.