Review on quantitative determination of preservatives - methyl paraben and propyl paraben by thin layer chromatography
Authors: P Vennela , S Asma Parveen , Y Prapurna Chandra 
Published: February 2025

In the field of analytical chemistry and various pharmaceutical fields the separation techniques are widely used to study specific compounds from a mixture or a complex material. One such separation technique is called Chromatography. A simple, rapid, and cost-effective TLC method was developed and validated for the quantitative determination of methyl paraben and propyl paraben in pharmaceutical and cosmetic products. The method employed a mobile phase consisting of ethyl acetate-methanol-water (80:10:10, v/v/v) and showed good linearity (R2 > 0.99), accuracy (recovery 95-105%), precision (RSD < 2%), and specificity. The limits of detection and quantitation were 0.1 μg and 0.5 μg, respectively. This TLC method can be used for quality control and regulatory purposes.

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