bluuebunny/crossref_metadata_2025 huggingface dataset:
An Efficient Computational Approach for Computing Subdivision Depth of Non-Stationary Binary Subdivision Schemes
Authors: Abdul Karim Samsul Ariffin , Khan Faheem , Mustafa Ghulam , Shahzad Aamir , Asghar Muhammad
Published: May 2023

Subdivision schemes are equipped with some rules that take a polygon as an input and produce smooth curves or surfaces as an output. This presents the issue of how accurately the polygon approximates the limit curve and surface. What number of iterations/levels would be necessary to achieve the required shape at a user-specified error tolerance? In fact, several methods have been introduced in the case of stationary schemes to address the issue in terms of the error bounds (distance between polygon/polyhedron and limiting shape) and subdivision depth (the number of iterations required to obtain the result at a user-specified error tolerance). However, in the case of non-stationary schemes, this topic needs to be further studied to meet the requirements of new practical applications. This paper highlights a new approach based on a convolution technique to estimate error bounds and subdivision depth for non-stationary schemes. The given technique is independent of any condition on the coefficient of the non-stationary subdivision schemes, and it also produces the best results with the least amount of computational effort. In this paper, we first associated constants with the vectors generated by the given non-stationary schemes, then formulated an expression for the convolution product. This expression gives real values, which monotonically decrease with the increase in the order of the convolution in both the curve and surface cases. This convolution feature plays an important role in obtaining the user-defined error tolerance with fewer iterations. It achieves a trade-off between the number of iterations and user-specified errors. In practice, more iterations are needed to achieve a lower error rate, but we achieved this goal by using fewer iterations.
Robust Evaluation and Comparison of EEG Source Localization Algorithms for Accurate Reconstruction of Deep Cortical Activity
Authors: Shen Hao , Yu Yuguo
Published: May 2023

Accurately reconstructing deep cortical source activity from EEG recordings is essential for understanding cognitive processes. However, currently, there is a lack of reliable methods for assessing the performance of EEG source localization algorithms. This study establishes an algorithm evaluation framework, utilizing realistic human head models and simulated EEG source signals with spatial propagations. We compare the performance of several newly proposed Bayesian algorithms, including full Dugh, thin Dugh, and Mackay, against classical methods such as MN and eLORETA. Our results, which are based on 630 Monte Carlo simulations, demonstrate that thin Dugh and Mackay are mathematically sound and perform significantly better in spatial and temporal source reconstruction than classical algorithms. Mackay is less robust spatially, while thin Dugh performs best overall. Conversely, we show that full Dugh has significant theoretical flaws that negatively impact localization accuracy. This research highlights the advantages and limitations of various source localization algorithms, providing valuable insights for future development and refinement in EEG source localization methods.
Scaling Limits of a Tandem Queue with Two Infinite Orbits
Authors: Nazarov Anatoly , Phung-Duc Tuan , Paul Svetlana , Morozova Mariya
Published: May 2023

This paper considers a tandem queueing network with a Poisson arrival process of incoming calls, two servers, and two infinite orbits by the method of asymptotic analysis. The servers provide services for incoming calls for exponentially distributed random times. Blocked customers at each server join the orbit of that server and retry to enter the server again after an exponentially distributed time. Under the condition of low retrial rates, we prove that the joint stationary distribution of scaled numbers of calls in the orbits weakly converges to a two-variable Normal distribution.
Computational Human Nasal Reconstruction Based on Facial Landmarks
Authors: Anh Tuan Ho Nguyen , Truong Thinh Nguyen
Published: May 2023

This research presented a mathematical-based approach to the computational reconstruction of the human nose through images with anthropometric characteristics. The nasal baselines, which were generated from facial aesthetic subunits combined with the facial landmarks, were reconstructed using interpolation and Mesh adaptive direct search algorithms to generate points that would serve as the support for the layer-by-layer reconstruction. The approach is proposed as the basis for nasal reconstruction in aesthetics or forensics rather than focusing on the applications of image processing or deep learning. A mathematical model for the computational reconstruction was built, and then volunteers were the subjects of nasal reconstruction experiments. The validations based on the area errors—which are based on four samples and eight sub-regions with different values depending on the regions C1, C2, and C3 and nasal shapes of the volunteers—were measured to prove the results of the mathematical model. Evaluations have demonstrated that the computer-reconstructed noses fit the original ones in shape and with minimum area errors. This study describes a computational reconstruction based on a mathematical approach directly to facial anthropometric landmarks to reconstruct the nasal shape.
New Vectors of TTX Analogues in the North Atlantic Coast: The Edible Crabs Afruca tangeri and Carcinus maenas
Authors: Lage Sandra , ten Brink Felicitas , Canário Adelino V. M. , Da Silva José P.
Published: May 2023

Tetrodotoxin (TTX) and its analogues are naturally occurring toxins historically responsible for human poisoning fatalities in Eastern Asia. It is typically linked to the consumption of pufferfish and, to a lesser extent, marine gastropods and crabs. In the scope of a comprehensive project to understand the prevalence of emergent toxins in edible marine organisms, we report, for the first time, the detection of TTX analogues in the soft tissues of edible crabs, the European fiddler crab (Afruca tangeri) and green crab (Carcinus maenas), harvested in southern Portugal. No TTX was detected in the analyzed samples. However, three TTX analogues were detected—an unknown TTX epimer, deoxyTTX, and trideoxyTTX. These three analogues were found in the European fiddler crab while only trideoxyTTX was found in the green crab, suggesting that the accumulation of TTX analogues might be influenced by the crabs’ different feeding ecology. These results highlight the need to widely monitor TTX and its analogues in edible marine species in order to provide adequate information to the European Food Safety Authority and to protect consumers.
Simulation of Cross Wedge Rolling and Hot Extrusion-Combined Forming Process for Axle Sleeve
Authors: Sun Wenhui , Yang Cuiping
Published: May 2023

In this paper, an axle sleeve is formed through a combined cross wedge rolling (CWR) and hot extrusion process, and the combined forming process is simulated via finite element analysis software Deform-3D. The forming mechanism is revealed by analyzing the stress and strain distribution, the temperature variation and the metal flow law of the workpiece during CWR and hot extrusion. Combined with CWR and hot extrusion forming experiments, the feasibility of a combined rolling and extrusion process to produce an axle sleeve is verified. It has been proven that the outer steps of the axle sleeve produced through the rolling extrusion composite process are well formed, the flange extrusion cavity is full, the metal streamline is continuous, the axis of the inner hole does not easily deviate, the product quality is good and the production efficiency is high.
Metabolites of Geum aleppicum and Sibbaldianthe bifurca: Diversity and α-Glucosidase Inhibitory Potential
Authors: Kashchenko Nina I. , Olennikov Daniil N. , Chirikova Nadezhda K.
Published: May 2023

α-Glucosidase inhibitors are essential in the treatment of diabetes mellitus. Plant-derived drugs are promising sources of new compounds with glucosidase-inhibiting ability. The Geum aleppicum Jacq. and Sibbaldianthe bifurca (L.) Kurtto & T.Erikss. herbs are used in many traditional medical systems to treat diabetes. In this study, metabolites of the G. aleppicum and S. bifurca herbs in active growth, flowering, and fruiting stages were investigated using high-performance liquid chromatography with photodiode array and electrospray ionization triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS/MS). In total, 29 compounds in G. aleppicum and 41 components in S. bifurca were identified including carbohydrates, organic acids, benzoic and ellagic acid derivatives, ellagitannins, flavonoids, and triterpenoids. Gemin A, miquelianin, niga-ichigoside F1, and 3,4-dihydroxybenzoic acid 4-O-glucoside were the dominant compounds in the G. aleppicum herb, while guaiaverin, miquelianin, tellimagrandin II2, casuarictin, and glucose were prevailing compounds in the S. bifurca herb. On the basis of HPLC activity-based profiling of the G. aleppicum herb extract, the most pronounced inhibition of α-glucosidase was observed for gemin A and quercetin-3-O-glucuronide. The latter compound and quercetin-3-O-arabinoside demonstrated maximal inhibition of α-glucosidase in the S. bifurca herb extract. The obtained results confirm the prospects of using these plant compounds as possible sources of hypoglycemic nutraceuticals.
A Compact Piezo-Inertia Actuator Utilizing the Double-Rocker Flexure Hinge Mechanism
Authors: Sun Pingping , Lei Chenglong , Ge Chuannan , Guo Yunjun , Zhu Xingxing
Published: May 2023

With a simple structure and control method, the piezo-inertia actuator is a preferred embodiment in the field of microprecision industry. However, most of the previously reported actuators are unable to achieve a high speed, high resolution, and low deviation between positive and reverse velocities at the same time. To achieve a high speed, high resolution, and low deviation, in this paper we present a compact piezo-inertia actuator with a double rocker-type flexure hinge mechanism. The structure and operating principle are discussed in detail. To study the load capacity, voltage characteristics, and frequency characteristics of the actuator, we made a prototype and conducted a series of experiment. The results indicate good linearity in both positive and negative output displacements. The maximum positive and negative velocities are about 10.63 mm/s and 10.12 mm/s, respectively, and the corresponding speed deviation is 4.9%. The positive and negative positioning resolutions are 42.5 nm and 52.5 nm, respectively. In addition, the maximum output force is 220 g. These results show that the designed actuator has a minor speed deviation and good output characteristics.
A Lightweight Method for Detecting IC Wire Bonding Defects in X-ray Images
Authors: Zhan Daohua , Lin Jian , Yang Xiuding , Huang Renbin , Yi Kunran , Liu Maoling , Zheng Hehui , Xiong Jingang , Cai Nian , Wang Han , Qiu Baojun
Published: May 2023

Integrated circuit (IC) X-ray wire bonding image inspections are crucial for ensuring the quality of packaged products. However, detecting defects in IC chips can be challenging due to the slow defect detection speed and the high energy consumption of the available models. In this paper, we propose a new convolutional neural network (CNN)-based framework for detecting wire bonding defects in IC chip images. This framework incorporates a Spatial Convolution Attention (SCA) module to integrate multi-scale features and assign adaptive weights to each feature source. We also designed a lightweight network, called the Light and Mobile Network (LMNet), using the SCA module to enhance the framework’s practicality in the industry. The experimental results demonstrate that the LMNet achieves a satisfactory balance between performance and consumption. Specifically, the network achieved a mean average precision (mAP50) of 99.2, with 1.5 giga floating-point operations (GFLOPs) and 108.7 frames per second (FPS), in wire bonding defect detection.
Antibacterial Activity against Four Fish Pathogenic Bacteria of Twelve Microalgae Species Isolated from Lagoons in Western Greece
Authors: Androutsopoulou Chrysa , Makridis Pavlos
Published: May 2023

Microalgae may produce a range of high-value bioactive substances, making them a promising resource for various applications. In this study, the antibacterial activity of twelve microalgae species isolated from lagoons in western Greece was examined against four fish pathogenic bacteria (Vibrio anguillarum, Aeromonas veronii, Vibrio alginolyticus, and Vibrio harveyi). Two experimental approaches were used to evaluate the inhibitory effect of microalgae on pathogenic bacteria. The first approach used bacteria-free microalgae cultures, whereas the second approach used filter-sterilized supernatant from centrifuged microalgae cultures. The results demonstrated that all microalgae had inhibitory effects against pathogenic bacteria in the first approach, particularly 4 days after inoculation, where Asteromonas gracilis and Tetraselmis sp. (red var., Pappas) exhibited the highest inhibitory activity, reducing bacterial growth by 1 to 3 log units. In the second approach, Tetraselmis sp. (red var., Pappas) showed significant inhibition against V. alginolyticus between 4 and 25 h after inoculation. Moreover, all tested cyanobacteria exhibited inhibitory activity against V. alginolyticus between 21 and 48 h after inoculation. Statistical analysis was performed using the independent samples t-test. These findings suggested that microalgae produce compounds with antibacterial activity, which could be useful in aquaculture.
Molecular Characterization and Prevalence of Antimicrobial-Resistant Escherichia coli Isolates Derived from Clinical Specimens and Environmental Habitats
Authors: Dioli Chrysoula , Pappa Olga , Siatravani Eirini , Bratakou Spyridoula , Tatsiopoulos Apostolos , Giakkoupi Panagiota , Miriagou Vivi , Beloukas Apostolos
Published: May 2023

Antibiotic-resistant bacteria (ARB) are present in wastewaters as their elimination during treatment in wastewater treatment plants (WWTPs) is often impossible. Water plays an important role in the spread of these microorganisms among humans, animals and the environment. This study aimed to assess the antimicrobial resistance patterns, resistance genes and molecular genotypes by means of phylogenetic groups of E. coli isolates in aquatic habitats, including sewage and receiving water bodies, as well as clinical settings in the Boeotia regional district of Greece. The highest resistance rates among both environmental and clinical isolates were observed to be for penicillins, ampicillin and piperacillin. Resistance patterns related to extended spectrum β-lactamases (ESBL) production and ESBL genes were also detected in both environmental and clinical isolates. Phylogenetic group B2 was predominant in clinical settings and the second most frequent among wastewaters, whereas group A was dominant in all environmental isolates. In conclusion, the studied river water and wastewaters may serve as reservoirs of resistant E. coli isolates that pose potential threats to both human and animal health.
Cu–S Isotopes of the Main Sulfides and Indicative Significance in the Qibaoshan Cu–Au Polymetallic Ore District, Wulian County, Shandong Province, North China Craton
Authors: Sun Yuqin , Wang Xin , Zhang Yan , Li Dapeng , Shan Wei , Geng Ke , Wei Pengfei , Liu Qiang , Xie Wei , Chi Naijie
Published: May 2023

With a focus on the Cu isotope geochemistry of chalcopyrite, this paper analyzed the Cu isotope geochemistry of the Qibaoshan crypto-explosive breccia-type Cu–Au polymetallic ore district in Wulian, Shandong Province, North China Craton (NCC). Combined with the results of the in situ sulfur isotope analysis of sulfides, a certain reference and evidence for the study of the genetic mechanism of the epithermal-porphyry Cu polymetallic metallogenic system were provided. The results of the in situ isotope analysis show that the δ34S values of the main sulfides in the Qibaoshan Cu–Au polymetallic ore district range from −6.81‰ to +3.82‰ and are likely to be attributed to the mixing of the derived mantle with the surrounding sedimentary rock assimilation. The ore-forming mechanism may be related to the progressive cooling and transition of the earliest hydrothermal fluids that were dominated by H2S under relatively reducing conditions, followed by a gradual transition from oxidation to reduction. The Cu isotopic composition of the sulfides in ores (δ65Cu = +0.169‰–+0.357‰) decreases with depth, which is likely caused by the upward transport of heavier Cu isotopes. The upper part of the crypto-explosive breccia pipe in the Qibaoshan area may be relatively more gaseous, resulting in the enrichment of δ65Cu. As the gas phase decreases and the liquid phase increases with depth, the δ65Cu value gradually decreases. This indicates the transition from a low-temperature phyllic alteration to a high-temperature K-feldspar alteration. Large, concealed pluton intrusions or orebodies may be present at a depth of the Qibaoshan area. The heavy δ65Cu characteristic is a potential indicator for tracing the fluid activity of the porphyry system and searching for Cu mines. The results provide a reference for the study of the genetic mechanisms of the epithermal-porphyry Cu polymetallic metallogenic system.
The Transport of Charged Molecules across Three Lipid Membranes Investigated with Second Harmonic Generation
Authors: Xu Baomei , Li Jianhui , Zhang Shuai , Zeb Johar , Chen Shunli , Yuan Qunhui , Gan Wei
Published: May 2023

Subtle variations in the structure and composition of lipid membranes can have a profound impact on their transport of functional molecules and relevant cell functions. Here, we present a comparison of the permeability of bilayers composed of three lipids: cardiolipin, DOPG (1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol), and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)). The adsorption and cross-membrane transport of a charged molecule, D289 (4-(4-diethylaminostyry)-1-methyl-pyridinium iodide), on vesicles composed of the three lipids were monitored by second harmonic generation (SHG) scattering from the vesicle surface. It is revealed that structural mismatching between the saturated and unsaturated alkane chains in POPG leads to relatively loose packing structure in the lipid bilayers, thus providing better permeability compared to unsaturated lipid bilayers (DOPG). This mismatching also weakens the efficiency of cholesterol in rigidifying the lipid bilayers. It is also revealed that the bilayer structure is somewhat disturbed by the surface curvature in small unilamellar vesicles (SUVs) composed of POPG and the conical structured cardiolipin. Such subtle information on the relationship between the lipid structure and the molecular transport capability of the bilayers may provide clues for drug development and other medical and biological studies.
Schisandra henryi—A Rare Species with High Medicinal Potential
Authors: Jafernik Karolina , Ekiert Halina , Szopa Agnieszka
Published: May 2023

Schisandra henryi (Schisandraceae) is a plant species endemic to Yunnan Province in China and is little known in Europe and America. To date, few studies, mainly performed by Chinese researchers, have been conducted on S. henryi. The chemical composition of this plant is dominated by lignans (dibenzocyclooctadiene, aryltetralin, dibenzylbutane), polyphenols (phenolic acids, flavonoids), triterpenoids, and nortriterpenoids. The research on the chemical profile of S. henryi showed a similar chemical composition to S. chinensis—a globally known pharmacopoeial species with valuable medicinal properties whichis the best-known species of the genus Schisandra. The whole genus is characterized by the presence of the aforementioned specific dibenzocyclooctadiene lignans, known as “Schisandra lignans”. This paper was intended to provide a comprehensive review of the scientific literature published on the research conducted on S. henryi, with particular emphasis on the chemical composition and biological properties. Recently, a phytochemical, biological, and biotechnological study conducted by our team highlighted the great potential of S. henryi in in vitro cultures. The biotechnological research revealed the possibilities of the use of biomass from S. henryi as an alternative to raw material that cannot be easily obtained from natural sites. Moreover, the characterization of dibenzocyclooctadiene lignans specific to the Schisandraceae family was provided. Except for several scientific studies which have confirmed the most valuable pharmacological properties of these lignans, hepatoprotective and hepatoregenerative, this article also reviews studies that have confirmed the anti-inflammatory, neuroprotective, anticancer, antiviral, antioxidant, cardioprotective, and anti-osteoporotic effects and their application for treating intestinal dysfunction.
Aqueous Binary Mixtures of Stearic Acid and Its Hydroxylated Counterpart 12-Hydroxystearic Acid: Cascade of Morphological Transitions at Room Temperature
Authors: Almeida Maëva , Dudzinski Daniel , Amiel Catherine , Guigner Jean-Michel , Prévost Sylvain , Le Coeur Clémence , Cousin Fabrice
Published: May 2023

Here, we describe the behavior of mixtures of stearic acid (SA) and its hydroxylated counterpart 12-hydroxystearic acid (12-HSA) in aqueous mixtures at room temperature as a function of the 12-HSA/SA mole ratio R. The morphologies of the self-assembled aggregates are obtained through a multi-structural approach that combines confocal and cryo-TEM microscopies with small-angle neutron scattering (SANS) and wide-angle X-ray scattering (WAXS) measurements, coupled with rheology measurements. Fatty acids are solubilized by an excess of ethanolamine counterions, so that their heads are negatively charged. A clear trend towards partitioning between the two types of fatty acids is observed, presumably driven by the favorable formation of a H-bond network between hydroxyl OH function on the 12th carbon. For all R, the self-assembled structures are locally lamellar, with bilayers composed of crystallized and strongly interdigitated fatty acids. At high R, multilamellar tubes are formed. The doping via a low amount of SA molecules slightly modifies the dimensions of the tubes and decreases the bilayer rigidity. The solutions have a gel-like behavior. At intermediate R, tubes coexist in solution with helical ribbons. At low R, local partitioning also occurs, and the architecture of the self-assemblies associates the two morphologies of the pure fatty acids systems: they are faceted objects with planar domains enriched in SA molecules, capped with curved domains enriched in 12-HSA molecules. The rigidity of the bilayers is strongly increased, as well their storage modulus. The solutions remain, however, viscous fluids in this regime.
Formal [3 + 2] Cycloaddition of α-Imino Esters with Azo Compounds: Facile Construction of Pentasubstituted 1,2,4-Triazoline Skeletons
Authors: Yoshida Yasushi , Ida Hidetoshi , Mino Takashi , Sakamoto Masami
Published: May 2023

1,2,4-Triazole and 1,2,4-triazoline are important components of bioactive molecules and catalysts employed in organic synthesis. Therefore, the efficient synthesis of these components has received significant research attention. However, studies on their structural diversity remain lacking. Previously, we developed chiral phase-transfer-catalyzed asymmetric reactions of α-imino carbonyl compounds with α,β-unsaturated carbonyl compounds and haloalkanes. In this study, we demonstrate the formal [3 + 2] cycloaddition reaction of α-imino esters with azo compounds under Brønsted base catalysis, resulting in the corresponding 1,2,4-triazolines in high yields. The results revealed that a wide range of substrates and reactants can be applied, irrespective of their steric and electronic characteristics. The present reaction made the general preparation of 3-aryl pentasubstituted 1,2,4-triazolines possible for the first time. Furthermore, a mechanistic study suggested that the reaction proceeds without isomerization into the aldimine form.
Side Streams of Vegetable Processing and Its Bioactive Compounds Support Microbiota, Intestine Milieu, and Immune System
Authors: Fotschki Joanna , Ogrodowczyk Anna M. , Wróblewska Barbara , Juśkiewicz Jerzy
Published: May 2023

The industry of vegetable processing generates large amounts of by-products, which often emerge seasonally and are susceptible to microbial degradation. Inadequate management of this biomass results in the loss of valuable compounds that are found in vegetable by-products that can be recovered. Considering the possibility of using waste, scientists are trying to reuse discarded biomass and residues to create a product of higher value than those processed. The by-products from the vegetable industry can provide an added source of fibre, essential oils, proteins, lipids, carbohydrates, and bioactive compounds, such as phenolics. Many of these compounds have bioactive properties, such as antioxidative, antimicrobial, and anti-inflammatory activity, which could be used, especially in the prevention or treatment of lifestyle diseases connected with the intestinal milieu, including dysbiosis and immune-mediated diseases resulting in inflammation. This review summarises the key aspects of the health-promoting value of by-products and their bioactive compounds derived from fresh or processed biomass and extracts. In this paper, the relevance of side streams as a source of beneficial compounds with the potential for promoting health is considered, particularly their impact on the microbiota, immune system, and gut milieu because all of these fields interact closely to affect host nutrition, prevent chronic inflammation, and provide resistance to some pathogens.
Ultrasound-Assisted Mineralization of 2,4-Dinitrotoluene in Industrial Wastewater Using Persulfate Coupled with Semiconductors
Authors: Chen Wen-Shing , Hsu Min-Chih
Published: May 2023

Oxidative degradation of 2,4-dinitrotoluenes in aqueous solution was executed using persulfate combined with semiconductors motivated by ultrasound (probe type, 20 kHz). Batch-mode experiments were performed to elucidate the effects of diverse operation variables on the sono-catalytic performance, including the ultrasonic power intensity, dosage of persulfate anions, and semiconductors. Owing to pronounced scavenging behaviors caused by benzene, ethanol, and methanol, the chief oxidants were presumed to be sulfate radicals which originated from persulfate anions, motivated via either the ultrasound or sono-catalysis of semiconductors. With regard to semiconductors, the increment of 2,4-dinitrotoluene removal efficiency was inversely proportional to the band gap energy of semiconductors. Based on the outcomes indicated in a gas chromatograph–mass spectrometer, it was sensibly postulated that the preliminary step for 2,4-dinitrotoluene removal was denitrated into o-mononitrotoluene or p-mononitrotoluene, followed by decarboxylation to nitrobenzene. Subsequently, nitrobenzene was decomposed to hydroxycyclohexadienyl radicals and converted into 2-nitrophenol, 3-nitrophenol, and 4-nitrophenol individually. Nitrophenol compounds with the cleavage of nitro groups synthesized phenol, which was sequentially transformed into hydroquinone and p-benzoquinone.
Dual Drug-Loaded Nanoliposomes Encapsulating Curcumin and 5-Fluorouracil with Advanced Medicinal Applications: Self-Monitoring and Antitumor Therapy
Authors: Liu Yu-Shi , Song Jia-Wen , Zhong Wen-Xiao , Yuan Ming-Hao , Guo Yu-Rou , Peng Cheng , Guo Li , Guo Yi-Ping
Published: May 2023

Due to the presence of physiological barriers, it is difficult to achieve the desired therapeutic efficacy of drugs; thus, it is necessary to develop an efficient drug delivery system that enables advanced functions such as self-monitoring. Curcumin (CUR) is a naturally functional polyphenol whose effectiveness is limited by poor solubility and low bioavailability, and its natural fluorescent properties are often overlooked. Therefore, we aimed to improve the antitumor activity and drug uptake monitoring by simultaneously delivering CUR and 5-Fluorouracil (5-FU) in the form of liposomes. In this study, dual drug-loaded liposomes (FC–DP–Lip) encapsulating CUR and 5-FU were prepared by the thin-film hydration method; their physicochemical properties were characterized; and their biosafety, drug uptake distribution in vivo, and tumor cell toxicity were evaluated. The results showed that the nanoliposome FC–DP–Lip showed good morphology, stability, and drug encapsulation efficiency. It showed good biocompatibility, with no side effects on zebrafish embryonic development. In vivo uptake in zebrafish showed that FC–DP–Lip has a long circulation time and presents gastrointestinal accumulation. In addition, FC–DP–Lip was cytotoxic against a variety of cancer cells. This work showed that FC–DP–Lip nanoliposomes can enhance the toxicity of 5-FU to cancer cells, demonstrating safety and efficiency, and enabling real-time self-monitoring functions.
Biotech Application of Exopolysaccharides from Curvularia brachyspora: Optimization of Production, Structural Characterization, and Biological Activity
Authors: Menolli Rafael Andrade , Tessaro Fernando Henrique Galvão , do Amaral Alex Evangelista , de Melo Renan Henrique , dos Santos Jean Felipe , Iacomini Marcello , Smiderle Fhernanda Ribeiro , Mello Rosiane Guetter
Published: May 2023

C. brachyspora, a widespread dematiaceous fungus, was evaluated in this study to optimize the production of exopolysaccharides (CB-EPS). Optimization was performed using response surface methodology, and the best production yielded 75.05% of total sugar at pH 7.4, with 0.1% urea, after 197 h. The obtained CB-EPS showed typical signals of polysaccharides, which was confirmed by FT-IR and NMR. The HPSEC analysis indicated a polydisperse polymer, showing a non-uniform peak, with an average molar mass (Mw) of 24,470 g/mol. The major monosaccharide was glucose (63.9 Mol%), followed by mannose (19.7 Mol%), and galactose (16.4 Mol%). Methylation analysis encountered derivatives that indicated the presence of a β-d-glucan and a highly branched glucogalactomannan. CB-EPS was tested on murine macrophages to verify its immunoactivity, and the treated cells were able to produce TNF-α, IL-6, and IL-10. However, the cells did not produce superoxide anions or nitric oxide nor stimulated phagocytosis. The results demonstrated an indirect antimicrobial activity of macrophages by stimulating cytokines, showing another biotech applicability for the exopolysaccharides produced by C. brachyspora.
Wafer-Level Highly Dense Metallic Nanopillar-Enabled High-Performance SERS Substrates for Molecular Detection
Authors: Zeng Pei , Zheng Mengjie , Chen Hao , Chen Guanying , Shu Zhiwen , Chen Lei , Liang Huikang , Zhou Yuting , Zhao Qian , Duan Huigao
Published: May 2023

Seeking sensitive, large-scale, and low-cost substrates is highly important for practical applications of surface-enhanced Raman scattering (SERS) technology. Noble metallic plasmonic nanostructures with dense hot spots are considered an effective construction to enable sensitive, uniform, and stable SERS performance and thus have attracted wide attention in recent years. In this work, we reported a simple fabrication method to achieve wafer-scale ultradense tilted and staggered plasmonic metallic nanopillars filled with numerous nanogaps (hot spots). By adjusting the etching time of the PMMA (polymethyl methacrylate) layer, the optimal SERS substrate with the densest metallic nanopillars was obtained, which possessed a detection limit down to 10−13 M by using crystal violet as the detected molecules and exhibited excellent reproducibility and long-term stability. Furthermore, the proposed fabrication approach was further used to prepare flexible substrates; for example, a SERS flexible substrate was proven to be an ideal platform for analyzing low-concentration pesticide residues on curved fruit surfaces with significantly enhanced sensitivity. This type of SERS substrate possesses potential in real-life applications as low-cost and high-performance sensors.
Characteristics of Synaptic Function of Mesoporous Silica–Titania and Mesoporous Titania Lateral Electrode Devices
Authors: Desai Dhanashri Vitthal , Yang Jongmin , Lee Hyun Ho
Published: May 2023

In this paper, we have fabricated non-volatile memory resistive switching (RS) devices and analyzed analog memristive characteristics using lateral electrodes with mesoporous silica–titania (meso-ST) and mesoporous titania (meso-T) layers. For the planar-type device having two parallel electrodes, current–voltage (I–V) curves and pulse-driven current changes could reveal successful long-term potentiation (LTP) along with long-term depression (LTD), respectively, by the RS active mesoporous two layers for 20~100 μm length. Through mechanism characterization using chemical analysis, non-filamental memristive behavior unlike the conventional metal electroforming was identified. Additionally, high performance of the synaptic operations could be also accomplished such that a high current of 10−6 Amp level could occur despite a wide electrode spacing and short pulse spike biases under ambient condition with moderate humidity (RH 30~50%). Moreover, it was confirmed that rectifying characteristics were observed during the I–V measurement, which was a representative feature of dual functionality of selection diode and the analog RS device for both meso-ST and meso-T devices. The memristive and synaptic functions along with the rectification property could facilitate a chance of potential implementation of the meso-ST and meso-T devices to neuromorphic electronics platform.
Model of a GaAs Quantum Dot in a Direct Band Gap AlGaAs Wurtzite Nanowire
Authors: Barettin Daniele , Shtrom Igor V. , Reznik Rodion R. , Cirlin George E.
Published: May 2023

We present a study with a numerical model based on k→·p→, including electromechanical fields, to evaluate the electromechanical and optoelectronic properties of single GaAs quantum dots embedded in direct band gap AlGaAs nanowires. The geometry and the dimensions of the quantum dots, in particular the thickness, are obtained from experimental data measured by our group. We also present a comparison between the experimental and numerically calculated spectra to support the validity of our model.
The Association between Coffee and Tea Consumption at Midlife and Risk of Dementia Later in Life: The HUNT Study
Authors: Abbel Denise , Åsvold Bjørn Olav , Kolberg Marit , Selbæk Geir , Noordam Raymond , Skjellegrind Håvard Kjesbu
Published: May 2023

Background: Studies exploring the possible protective effect of coffee and tea consumption on dementia have shown inconsistent results so far. We aimed to investigate whether consumption of tea and different types of coffee at midlife are associated with dementia later in life and whether sex or ApoE4 influence such association. Methods: We included 7381 participants from the Norwegian HUNT Study. Self-reported questionnaires assessed daily consumption of coffee and tea at baseline. After 22 years, individuals 70 years or older were screened for cognitive impairment. Results: General coffee consumption and tea consumption was not associated with dementia risk. Compared to daily consumption of 0–1 cups of coffee, daily consumption of ≥8 cups of boiled coffee was associated with increased dementia risk in women (OR: 1.83, 95% CI: 1.10–3.04, p-value for trend = 0.03) and daily consumption of 4–5 cups of other types of coffee was associated with a decrease in dementia risk in men (OR: 0.48, 95% CI: 0.32–0.72, p-value for trend = 0.05). Furthermore, the association between boiled coffee and increased dementia risk was only found in ApoE4 non-carriers. Differences by sex or ApoE4 carrier status were not supported by strong statistical evidence for interaction. Tea consumption was not associated with dementia risk. Conclusion: type of coffee may play a role in the direction of the association between coffee-drinking habits and dementia later in life.
The Potential of Food Fortification as an Enabler of More Environmentally Sustainable, Nutritionally Adequate Diets
Authors: Grasso Alessandra C. , Besselink Julia J. F. , Tyszler Marcelo , Bruins Maaike J.
Published: May 2023

Policies encouraging shifts towards more plant-based diets can lead to shortfalls in micronutrients typically present in animal products (B-vitamins, vitamin D, calcium, iodine, iron, selenium, zinc, and long-chain omega-3 fatty acids). We modelled the effect of fortifying foods with these critical micronutrients, with the aim of achieving nutrition and sustainability goals, using food consumption data from Dutch adults (19–30 years). Three dietary scenarios were optimized for nutritional adequacy and 2030 greenhouse gas emissions (GHGE-2030) targets, respectively, with the fewest deviations from the baseline diet: (i) the current diet (mainly vitamin A- and D-fortified margarine, iodized bread, and some calcium- and vitamin D-fortified dairy alternatives and iron- and vitamin B12-fortified meat alternatives); (ii) all plant-based alternatives fortified with critical micronutrients; and (iii) fortified bread and oils. Optimizing the current diet for nutrition and GHGE-2030 targets reduced animal-to-plant protein ratios from ~65:35, to 33:67 (women) and 20:80 (men), but required major increases in legumes and plant-based alternatives. When fortifying all plant-based alternatives and, subsequently, bread and oil, smaller dietary changes were needed to achieve nutrition and GHGE-2030 targets. Fortifying food products with critical micronutrients, ideally with complementary education on plant-based foods, can facilitate the transition to healthier and more sustainable diets.
PEG 400:Trehalose Coating Enhances Curcumin-Loaded PLGA Nanoparticle Internalization in Neuronal Cells
Authors: Caballero-Florán Isaac , Cortés Hernán , Borbolla-Jiménez Fabiola , Florán-Hernández Carla , Del Prado-Audelo María , Magaña Jonathan , Florán Benjamín , Leyva-Gómez Gerardo
Published: May 2023

This work proposes a combination of polyethylene glycol 400 (PEG) and trehalose as a surface modification approach to enhance PLGA-based nanoparticles as a drug carrier for neurons. PEG improves nanoparticles’ hydrophilicity, and trehalose enhances the nanoparticle’s cellular internalization by inducing a more auspicious microenvironment based on inhibiting cell surface receptor denaturation. To optimize the nanoprecipitation process, a central composite design was performed; nanoparticles were adsorbed with PEG and trehalose. PLGA nanoparticles with diameters smaller than 200 nm were produced, and the coating process did not considerably increase their size. Nanoparticles entrapped curcumin, and their release profile was determined. The nanoparticles presented a curcumin entrapment efficiency of over 40%, and coated nanoparticles reached 60% of curcumin release in two weeks. MTT tests and curcumin fluorescence, with confocal imaging, were used to assess nanoparticle cytotoxicity and cell internalization in SH-SY5Y cells. Free curcumin 80 µM depleted the cell survival to 13% at 72 h. Contrariwise, PEG:Trehalose-coated curcumin-loaded and non-loaded nanoparticles preserved cell survival at 76% and 79% under the same conditions, respectively. Cells incubated with 100 µM curcumin or curcumin nanoparticles for 1 h exhibited 13.4% and 14.84% of curcumin’s fluorescence, respectively. Moreover, cells exposed to 100 µM curcumin in PEG:Trehalose-coated nanoparticles for 1 h presented 28% fluorescence. In conclusion, PEG:Trehalose-adsorbed nanoparticles smaller than 200 nm exhibited suitable neural cytotoxicity and increased cell internalization proficiency.
Cyclodextrins and Their Polymers Affect Human Serum Albumin’s Interaction with Drugs Used in the Treatment of Pulmonary Infections
Authors: Skuredina Anna A. , Yakupova Linara R. , Kopnova Tatiana Yu. , Le-Deygen Irina M. , Belogurova Natalya G. , Kudryashova Elena V.
Published: May 2023

Respiratory infectious diseases have challenged medical communities and researchers. Ceftriaxone, meropenem and levofloxacin are widely used for bacterial infection treatment, although they possess severe side effects. To overcome this, we propose cyclodextrin (CD) and CD-based polymers as a drug delivery system for the drugs under consideration. CD polymers demonstrate higher binding affinity for levofloxacin (Ka ≈ 105 M) compared to drug–CD complexes. CDs slightly alter the drugs’ affinity for human serum albumin (HSA), whereas CD polymers increase the drugs’ binding affinity up to 100 times. The most significant effect was observed for more the hydrophilic drugs ceftriaxone and meropenem. The drug’s encapsulation in CD carriers leads to a decrease in the degree of change in the protein’s secondary structure. The drug–CD carrier–HSA complexes demonstrate satisfying antibacterial activity in vitro, and even a high binding affinity does not decrease the drug’s microbiological properties after 24 h. The proposed carriers are promising for a drug form with a prolonged drug release.
Ends of Life: Forms of Life as the “Ruins of an Enduring Fable”?
Authors: Guetti Edward
Published: May 2023

This paper addresses the possibility of using the Wittgensteinian conception of “forms of life” (“Lebensformen”) as a potentially transformative philosophical framework that responds to contemporary challenges. These challenges can be understood as resulting from parallel discourses of “ends”: that of “nature” and that of the “human”. These challenges are relevant, especially, for a Cavellian interpretation of Wittgensteinian Lebensformen as an expression of cultural and natural factors. My purpose is to show how Cavell’s elaboration of Wittgensteinian Lebensformen can be maintained against the critical pressures exerted by prevailing discourses of ends.
Variation in Leaf Volatile Emissions in Potato (Solanum tuberosum) Cultivars with Different Late Blight Resistance
Authors: Agho C. A. , Runno-Paurson E. , Tähtjärv T. , Kaurilind E. , Niinemets Ü.
Published: May 2023

Volatile organic compounds (VOCs) play key roles in plant abiotic and biotic stress resistance, but even for widespread crops, there is limited information on variations in the magnitude and composition of constitutive VOC emissions among cultivars with varying stress resistance. The foliage VOC emissions from nine local and commercial potato cultivars (Alouette, Sarme, Kuras, Ando, Anti, Jõgeva Kollane, Teele, 1681-11, and Reet) with medium to late maturities and varying Phytophthora infestans (the causative agent of late blight disease) resistance backgrounds were analyzed to gain an insight into the genetic diversity of constitutive VOC emissions and to test the hypothesis that cultivars more resistant to Phytophthora infestans have greater VOC emissions and different VOC fingerprints. Forty-six VOCs were identified in the emission blends of potato leaves. The majority of the VOCs were sesquiterpenes (50% of the total number of compounds and 0.5–36.9% of the total emissions) and monoterpenes (30.4% of the total number of compounds and 57.8–92.5% of the total VOC emissions). Qualitative differences in leaf volatiles, mainly in sesquiterpenes, were related to the potato genotype background. Among the volatile groups, the monoterpenes α-pinene, β-pinene, Δ3-carene, limonene, and p-cymene, the sesquiterpenes (E)-β-caryophyllene and α-copaene, and green leaf volatile hexanal were the major volatiles in all cultivars. A higher share of VOCs known to have antimicrobial activities was observed. Interestingly, the cultivars were grouped into high and low resistance categories based on the VOC profiles, and the total terpenoid and total constitutive VOC emission scale positively with resistance. To support and expedite advances in breeding for resistance to diseases such as late blight disease, the plant research community must develop a fast and precise approach to measure disease resistance. We conclude that the blend of emitted volatiles is a fast, non-invasive, and promising indicator to identify cultivars resistant to potato late blight disease.
Genome-Wide Identification and Expression Analysis of the VILLIN Gene Family in Soybean
Authors: Zhou Yueqiong , He Liangliang , Zhou Shaoli , Wu Qing , Zhou Xuan , Mao Yawen , Zhao Baolin , Wang Dongfa , Zhao Weiyue , Wang Ruoruo , Hu Huabin , Chen Jianghua
Published: May 2023

The VILLIN (VLN) protein is an important regulator of the actin cytoskeleton, which orchestrates many developmental processes and participates in various biotic and abiotic responses in plants. Although the VLN gene family and their potential functions have been analyzed in several plants, knowledge of VLN genes in soybeans and legumes remains rather limited. In this study, a total of 35 VLNs were characterized from soybean and five related legumes. Combining with the VLN sequences from other nine land plants, we categorized the VLN gene family into three groups according to phylogenetic relationships. Further detailed analysis of the soybean VLNs indicated that the ten GmVLNs were distributed on 10 of the 20 chromosomes, and their gene structures and protein motifs showed high group specificities. The expression pattern analysis suggested that most GmVLNs are widely expressed in various tissues, but three members have a very high level in seeds. Moreover, we observed that the cis−elements enriched in the promoters of GmVLNs are mainly related to abiotic stresses, hormone signals, and developmental processes. The largest number of cis−elements were associated with light responses, and two GmVLNs, GmVLN5a, and GmVLN5b were significantly increased under the long light condition. This study not only provides some basic information about the VLN gene family but also provides a good reference for further characterizing the diverse functions of VLN genes in soybeans.
Adhesion Properties and Stability of Non-Polar Polymers Treated by Air Atmospheric-Pressure Plasma
Authors: Bîrleanu Emma , Mihăilă Ilarion , Topală Ionuț , Borcia Cătălin , Borcia Gabriela
Published: May 2023

Atmospheric-pressure plasma (APP) has advantages for enhancing the adhesion of polymers and has to provide uniform, efficient treatment, which also limits the recovery effect of treated surfaces. This study investigates the effects of APP treatment on polymers that have no oxygen bonded in their structure and varying crystallinity, aiming to assess the maximum level of modification and the post-treatment stability of non-polar polymers based on their initial structure parameters, including the crystalline–amorphous structure. An APP reactor simulating continuous processing operating in air is employed, and the polymers are analyzed using contact angle measurement, XPS, AFM, and XRD. APP treatment significantly enhances the hydrophilic character of the polymers, with semicrystalline polymers exhibiting adhesion work values of approximately 105 mJ/m2 and 110 mJ/m2 for 0.5 s and 1.0 s exposure, respectively, while amorphous polymers reach approximately 128 mJ/m2. The maximum average oxygen uptake is around 30%. Short treatment times induce the roughening of the semicrystalline polymer surfaces, while the amorphous polymer surfaces become smoother. The polymers exhibit a limit to their modification level, with 0.5 s exposure being optimal for significant surface property changes. The treated surfaces remain remarkably stable, with the contact angle only reverting by a few degrees toward that of the untreated state.