Tepung okra (Albelmoschus esculantus) menurunkan rasio kadar LDL terhadap HDL tikus hiperkolesterolemia
Authors: Febriyatna Ayu , Widiyawati Agatha 
Published: October 2018

<p><strong>ABSTRACT</strong><strong></strong></p><p><strong><em>Backgounds:</em></strong><em> Hypercholesterolemia is one cause of heart disease with high levels of Low Density Lipoprotein (LDL) cholesterol and low levels of high density lipoprotein (HDL) cholesterol. Okra (</em><em>A</em><em>lbelmoschus esculantus) </em><em>flour contain</em><em> high</em><em> fibers and more bioactive compound such as flavonoids. </em><em></em></p><p><strong><em>Objectives:</em></strong><em> </em><em>This study aimed to examine</em> <em>the effect</em><em>iveness of</em><em> okra flour against the </em><em>Low Density Lipoprotein (LDL) cholesterol level</em><em> </em><em>and high density lipoprotein (HDL) cholesterol level</em><em> </em><em>in Hypercholesterole</em><em>mic </em><em>rats. </em></p><p><strong><em>Methods:</em></strong><em> </em><em>Design of the </em><em>study was experimental design with pre-and posttest control goup</em><em>.</em><em> </em><em>Male white rats (rattus norvegicus) aged 8-10 weeks with body weight of 150-200 g were used, then divided into 4 goups of 8 rats. </em><em> Rats made Hypercholesterolemia used Haight Fat Diet (HFD) feed at a doses 4mg/100 g </em><em>body weight </em><em>for 14 days. </em><em>After that</em><em>,</em><em> </em><em>Hypercholesterolemic rats were provided by standard diet</em><em> rats given standart feed and okra flour with different doses it was goups positive control (KP) not given flour okra, okra flour </em><em>doses </em><em>0.09 g/200 g </em><em>bofy weight (P1)</em><em>, </em><em>okra flour doses </em><em>0.</em><em>18</em><em> g/200 g </em><em>body weight (P2), and </em><em>okra flour doses </em><em>0.</em><em>36</em><em> g/200 g </em><em>body weight (P3).</em><em> </em><em>The data was analyzed using </em><em>One Way ANOVA</em><em> </em><em>dan</em><em> </em><em>paired t-test</em><em>.</em></p><p><strong><em>Results:</em></strong><em> </em><em>Rats in all goup did not experience changes</em><em> in body weight significantly. Concentration of HDL cholesterol  increase</em><em>d</em><em> significantly in P3 goups with doses okra flour </em><em>0.36  g/200 g </em><em>body weight</em><em> compared withe the other goups. P3 goups</em> <em>attenuated the</em><em> conc</em><em>entration of LDL </em><em>cholesterol. </em></p><p><strong><em>Conclusions: </em></strong><em>the administration okra flour </em><em>(albelmoschus esculantus) at doses </em><em>0.36 g/200 g</em><em> </em><em>body weight</em><em> improve</em><em>d</em><em> HDL cholesterol and decrease</em><em>d</em><em> LDL cholesterol concentration in Hypercholesterolemi</em><em>c </em><em>rat</em><em>s</em><em> model .</em></p><p><strong><em> </em></strong></p><p><strong><em>KEYWORDS</em></strong><em>: </em><em>Hypercholesterolemi</em><em>c</em><em> High Density Lipoprotein (HDL</em><em>)</em><em>, Low Density Lipoprotein (LDL) and </em><em>Okra Flour</em><em>.</em><em> </em></p><p><strong><em>ABSTRAK </em></strong></p><p><strong> </strong></p><p class="Default"><strong><em>Latar belakang:</em></strong><em> Hiperkolesterolemia </em><em>adalah </em><em>salah satu penyebab penyakit jantung yang ditandai dengan kadar kolesterol Low Density Lipoprotein (LDL)</em><em> yang tinggi</em><em> </em><em>dan</em><em> kadar kolesterol High Density Lipoprotein (HDL) yang rendah. Okra (</em><em>A</em><em>lbelmoschus esculantus)</em><em> dalam bentuk tepung mengandung</em><em> </em><em>serat yang </em><em>tinggi </em><em>dan </em><em>kandungan </em><em>bioaktif seperti </em><em>flavonoid. </em><em></em></p><p class="Default"><strong><em>Tujuan</em></strong><strong><em>:</em></strong><em> </em><em>Pe</em><em>nelitian ini </em><em>untuk</em><em> mengetahui efektifitas tepung okra terhadap perubahan kadar kolesterol HDL dan LDL pada tikus putih model hiperkolesterolemia. </em></p><p class="Default"><strong><em>Metode:</em></strong><em> Desain penelitian ini yaitu true experimental dengan Kelompok Kontrol (Pretest-Posttest with Control Goup). </em><em>T</em><em>ikus putih </em><em>jantan (</em><em>Rattus novergicus</em><em>) </em><em>yang</em><em> berumur 8-10 minggu dengan bobot antara 150-200 g</em><em> dibagi kedalam 4 kelompok.</em><em> Tikus dibuat hiperkolesterolemia menggunakan pakan HFD dengan dosis 4 mg/100 g BB tikus perhari selama 14 hari. Selanjutnya, tikus hiperkolesterolemia diberi pakan pakan standart dan tepung okra dengan dosis yang berbeda yaitu k</em><em>elompok </em><em>KP (Kontrol Positif) hanya diberi pakan standar,</em><em> </em><em>kelompok P1 </em><em>(</em><em>tepung okra dosis </em><em>0</em><em>,</em><em>09 g/200 g BB tikus perhari</em><em>)</em><em>,</em><em> </em><em>k</em><em>elompok P2 </em><em>(</em><em>tepung okra dosis </em><em>0.18 g/200 g BB tikus perhari</em><em>),</em><em> dan kelompok P3</em><em> (</em><em>tepung okra dosis </em><em>0</em><em>,</em><em>36  g/200 g BB tikus perhari</em><em>)</em><em> selama 10 hari</em><em>. Data dianalisis menggunakan One Way ANOVA</em><em> </em><em>dan</em><em> </em><em>paired t-test</em><em>. </em></p><p class="Default"><strong><em>Hasil:</em></strong><em> Ti</em><em>kus percobaan pada </em><em>semua </em><em>kelompok </em><em>tidak terjadi perubahan berat badan yang signifikan. Kadar kolesterol HDL mengalami peningkatan yang signifikan pada kelompok </em><em>P3</em><em> dengan dosis</em><em> </em><em>tepung okra </em><em>0.36  g/200 g dibandingkan dengan kelompok yang lainnya.</em><em> Kelompok P3 juga mampu menurunkan kadar kolesterol LDL.</em></p><p class="Default"><strong><em>Kesimpulan: </em></strong><em>tepung okra </em><em>(albelmoschus esculantus) </em><em>pada dosis  0.36 g/200 g</em><em> BB tikus/hari</em><em> </em><em>selama 10 hari secara bermakna</em><em> </em><em>berpengaruh terhadap </em><em>peningkatan kadar kolesterol HDL dan </em><em>penurunan kadar  kolesterol </em><em>LDL </em><em>tikus</em><em> model hiperkolesterolemia</em><em></em></p><p class="Default"><strong><em> </em></strong></p><p><strong>KATA KUNCI</strong><em>: Hiperkolestrolemia, High Density Lipoprotein (HDL</em><em>)</em><em>, Low Density Lipoprotein (LDL), tepung okra</em></p>