bluuebunny/crossref_metadata_2025 huggingface dataset:
Kajian Kualitas Pelayanan Bus Primajasa Kelas Bisnis Rute Garut-Bekasi Pada Masa Pandemi Covid-19 Menggunakan Metode Fuzzy-Servqual
Authors: Prabowo Opik Legiman , Prasetyanto Dwi
Published: June 2023

Seiring dengan meningkatnya mobilitas kebutuhan penduduk ke luar kota, menuntut ketersediaan angkutan umum antar kota yang memenuhi syarat kelancaran, kenyamanan, dan keamanan. Masa pandemi Covid-19 sangat mempengaruhi keberlangsungan suatu perusahaan jasa Transportasi. Oleh karena itu tujuan penelitian adalah mengevaluasi kualitas pelayanan transportasi dari perusahaan-perusahaan bus yang ada di kota Garut untuk memenuhi tingkat pelayanan terbaik dari perusahaan di masa pandemi Covid-19. Penelitian menggunakan metode kuantitatif, dipilih angkutan umum bus antar kota perusahaan bus Primajasa cabang Garut. Penelitian kualitas pelayanan bus Primajasa Kelas Bisnis Rute Garut-Bekasi dengan jenis armada bus besar. Data didapatkan mengunakan metode survei dan wawancara langsung serta penyebaran kuesioner kepada pihak terkait. Penelitian kinerja pelayanan Bus Primajasa diukur dari ekspektasi dan persepsi kinerja berdasarkan survei dan menggunakan Metode Fuzzy-Servqual. Hasil penelitian dengan analisis Fuzzy-Servqual terdapat tiga belas atribut yang sudah sesuai antara persepsi dan harapan konsumen Bus dengan nilai gap positif. Kepuasan konsumen dan pelayanan di dalam bus pada atribut jumlah penumpang sesuai kapasitas angkut, tempat duduk penumpang nyaman, dilengkapi AC, tersedia kursi bagi penumpang berkebutuhan khusus, tersedia tempat sampah, memiliki rasa aman dan nyaman, kedisiplinan pengemudi, tersedia alat pemadam kebakaran, tersedianya fasilitas dan penerapan protokol kesehatan, transaksi cepat, kemudahan penumpang dalam menyampaikan keluhan/saran. Prioritas perbaikan pelayanan berdasarkan gap servqual score terbesar terdapat pada jadwal operasional sesuai yang telah ditetapkan.
Penerapan Metode Earned Value Analysis Terhadap Waktu Penjadwalan
Authors: Janizar Syapril
Published: June 2023

Manajemen proyek adalah suatu aktivitas yang didalamnya terdiri atas kegiatan merencanakan, memimpin, mengorganisir, serta mengendalikan sumberdaya organisasi perusahaan untuk mencapai tujuan organisasi yang telah ditentukan dalam jangka waktu tertentu dengan sumber daya tertentu. Studi kasus pada Proyek Pembangunan Gedung Ruang Kelas Baru (RKB) Madrasah Aliyah Negeri (MAN) Kab. Karawang yang berlokasi Karawang, Jawa Barat 41314. Menganalisis proyek menggunakan konsep Nilai Hasil (Earned Value Analysis) yang dititik beratkan pada waktu pelaksanaan Proyek Pembangunan Gedung Ruang Kelas Baru (RKB) Madrasah Aliyah Negeri (MAN) Kab. Karawang. Mengetahui dan menambah wawasan tentang pengendalian biaya dan waktu pada suatu proyek dengan penerapan Earned Value Analysis. Waktu penyelesaian pekerjaan sudah sesuai dengan rencana pekerjaan sehingga pekerjaan dapat selesai sesuai dengan jadwal.
Possible role of glucose-6-phosphatase 3 in the pathogenesis of uterine leiomyomas
Authors: Marcolongo Paola , Barone Virginia , Luisi Stefano
Published: June 2023

Background and aim: Glucose-6-phosphatase catalytic subunit 3 (G6PC3) has been recently described as a metabolite repair enzyme involved in the disposal of the phosphorylated glucose analog 1,5-anhydroglucitol-6-phosphate (1,5AG6P). This function is especially relevant in neutrophils; indeed, G6PC3 deficiency leads to neutropenia as the accumulated metabolite 1,5AG6P inhibits the first step of glycolysis. Like neutrophils, tumoral metabolism also mainly relies on glycolysis, and we wondered if G6PC3 is expressed in uterine leiomyoma samples and if it can eventually have a role in the pathogenesis of these tumors. Understanding the complex pathophysiology of leiomyomas is a prerequisite to develop new therapeutic strategies.
Methods: We used human uterine leiomyoma and matched myometrial samples. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) were performed.
Results: Immunohistochemical analysis has not evidenced appreciable differences between pathologic versus normal tissue samples. Indeed, qPCR analysis suggests a higher expression of G6PC3 in human uterine leiomyoma than in matched myometrial samples.
Conclusion: A targeted therapeutic inhibition of G6PC3 in uterine leiomyoma samples is a potential strategy to slow down tumor growth.
The effect of ovarian hormones on antioxidant enzyme activities in the brain of male rats
Authors: Pajović SB , Saičić ZS , Spasić MB , Petrović VM
Published: June 2023

The brain is widely responsive to gonadal hormones. The functional significance of ovarian hormones in the brain is evident from biochemical studies indicating that estradiol or progesterone treatment of testectomized rats produces changes of antioxidant enzyme activities. The effect of estradiol benzoate (EB) and progesterone (P) in the control of antioxidant (AO) enzyme activities was studied in the brain of adult male Wistar rats. The activities of catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and glutathione reductase (GR) were measured in appropriate subcellular fractions, prepared from brains of animals belonging to various experimental groups. These groups were designed with the intention to follow changes in enzyme activities 2 h or 24 h after systemic administration of 5 g EB or 2 mg P to testectomized (TX) animals. The obtained results show that both EB and P increase CAT activity, whereas EB decreases GSH-Px, GST and GR activities. These findings clearly show the modulatory role of EB and P in the control of enzymes responsible for the protection of rat nerve cells against oxidative damage caused by free oxygen radicals.
Apoptotic DNA alterations in pig leukocytes after phagocytosis of bacteria are linked to maturation of the immune system
Authors: Matalová E , Španová A , Kovářů F
Published: June 2023

The effect of phagocytosis of living bacteria on apoptotic DNA changes was examined in pig leukocytes in relation to immune system maturation. Blood samples of pigs (aged 6, 12 and 18 weeks) were cultivated with a suspension of bacterial cells Salmonella typhimurium LB 5000 at 37 C. In the experimental groups, killed bacteria and microspheric particles were used to detect the influence of the phagocytic process. Phagocytic activity and index were determined in each sample by means of microspheric particles. The ability to kill engulfed microbes (bactericidal capacity) was estimated from the decrease in bacterial colony-forming units (CFU). Samples of cultured cells were taken for DNA analysis at given intervals. DNA ladder assay was used for qualitative apoptotic DNA break detection and the TUNEL AP test was employed for quantification of apoptosis. In 18-week-old animals, spontaneous DNA degradation was observed in the control group without phagocytosis after 8 h. In contrast, cells cultivated with microspheric particles or killed bacteria became apoptotic after 4 h. The rate of apoptotic DNA degradation was decreased in the group exposed to living bacteria. This prolonged survival of phagocytes was also detected in 12-week-old animals, but not at 6 weeks of age. These findings were supported by the ability of phagocytes in 6-week-old animals to engulf microbes, but their killing (bactericidal) ability was significantly decreased in comparison with other stages of immune system maturation. These results suggest that the process of phagocytosis itself is accompanied by activation of the apoptotic program in phagocytic cells of the pig immune system, but the presence of phagocyted living bacteria can delay this activation. The prolonged survival of short-lived cells was only observed in later phases of immune system maturation.
Pregnanolone isomers, pregnenolone and their polar conjugates around parturition
Authors: Klak J , Hill M , Pařízek A , Havlíková H , Bičíková M , Hampl R , Fait T , Šulcová J , Pouzar V , Kancheva R , Stárka L
Published: June 2023

The levels of four pregnanolone isomers and their polar conjugates and pregnenolone sulfate were measured in the plasma of 13 and 7 women at delivery with subarachnoidal and epidural analgesia, respectively, and in corresponding samples of umbilical plasma using a simple quadrupole GC/MS system with electron impact ionization (pregnenolone isomers), RIA following HPLC separation (pregnenolone) and specific RIA (pregnanolone sulfate). The concentration of epipregnanolone (3b-hydroxy-5b-pregnan-20-one) in both maternal and umbilical plasma was much lower than that of other pregnanolone isomers. The levels of 3b-hydroxy-pregnanolone isomers were significantly higher in the umbilical plasma than in the maternal, while the differences in 3a-hydroxy-isomers were insignificant. The differences in conjugates were insignificant with the exception of allopregnanolone, the levels of which were lower in umbilical plasma. In all the pregnanolone isomers, a significantly lower conjugated/unconjugated steroid ratio was found in the umbilical plasma than in the maternal plasma. In addition, time profiles of the steroids were measured around parturition and in the postpartum period in the maternal serum. Similarly, the levels of polar conjugates of all pregnanolone isomers were followed during parturition. Changes in concentrations of free steroids exhibited a similar pattern, with a fall primarily within the first hour after delivery. The decrease in conjugated steroids was shifted to the interval within the first hour and first day after delivery, and the changes were more pronounced. The time profiles of the conjugated/free steroid ratio exhibited a significant decrease within the first hour and the first day after delivery in all of the isomers investigated. A decrease was also observed in the ratio of 3a/3b- isomers and 5a/5b- isomers around parturition. The possible physiological consequences of the findings are indicated.
MODEL PEMBELAJARAN COPERATIVE LEARNING TIPE NUMBER HEAD TOGETHER SEBAGAI SOLUSI UNTUK MENINGKATKAN HASIL BELAJAR IPA SISWA SEKOLAH DASAR
Authors: Sugiharti Rini Endah , Aey Aey Rahayu
Published: June 2023

Mata pelajaran Ilmu Pengetahuan Alam merupakan salah satu mata pelajaran yang harus diajarkan di sekolah dasar sesuai dengan kurikulum pendidikan sekolah dasar. Dalam proses pembelajaran IPA lebih menitik beratkan pada serangkaian proses penyelidikan tentang suatu konsep peristiwa yang terjadi di sekitar siswa. Begitu pentingnya pembelajaran IPA dalam kehidupan, seharusnya mata pelajaran IPA menjadi mata pelajaran yang menarik. Tetapi masih banyak siswa yang tidak menyukai pelajaran ini, karena beranggapan terlalu banyak menghapal sesuatu yang tidak penting. Permasalahan di atas dapat diatasi dengan menggunakan Model Pembelajaran Number Head Together merupakan model yang mengutamakan kerjasama antara siswa, sehingga siswa bekerja sama untuk menyelesaikan permasalah (soal) secara bersama-sama, sehingga terjalin interaksi, komunikasi, dan sosialisasi yang positif dalam menyelesaikan permasalahan.Langkah-langkah model Number Head Together sebagai berikut 1) perencanaan, 2) pelaksanaan, 3) evaluasi. Hasil dengan menggunakan model pembelajaran Number Head Together, siswa diyakini dapat meningkatkan hasil belajar IPA dalam indikator penilaian hasil belajar ranah kognitif dari kemampuan (C1) mengingat hingga (C6) evaluasi.
IMPLEMENTASI MODEL PEMBELAJARAN BERUPA MEDIA UNTUK MENGATASI KESULITAN BELAJAR IPS SISWA ( Studi Kasus di Kelas V SD/MI )
Authors: Qalbi Fadhilla Afifah , Lasari Yufi Latmini , Anastasha Desty Ayu
Published: June 2023

Penelitian ini bertujuan untuk mendapatkan data tentang kesulitan belajar siswa yang dilatar belakangi oleh model pembelajaran dan juga media pembelajaran. Jenis daripenelitian ini adalah kualitatif, cara dari pengumpulan data ini yaitu dengan caraobservasi dan juga wawancata dari seorang guru serta siswa SD. Hasil dari analisis datatersebut yaitu masih banyak siswa SD yang kurang memahami dan fokus dalampelajaran IPS dengan model pembelajaran dan media pembelajaran yang di terapkanoleh guru. Upaya yang harus dilakukan yaitu membuat media pembelajaran semenarikmungkin dan dapat di variasikan agar siswa SD tidak bosan dalam pembelajaran yangberlangsung dan dapat fokus dalam belajar.
PERANAN MODEL THINK TALK WRITE (TTW) DALAM MENINGKATKAN KETERAMPILAN MENULIS KARANGAN DESKRIPSI SISWA SD PADA MATA PELAJARAN BAHASA INDONESIA
Authors: Rikmasari Rima , Kurniati Putri Rizki
Published: June 2023

Bahasa Indonesia merupakan mata pelajaran wajib yang dipelajari diseluruh jenjang pendidikan dasar sampai jenjang pendidikan tinggi di Indonesia. Karena Bahasa Indoensia juga mempunyai peranan penting dalam kehidupan manusia untuk mengusai ilmu dan teknologi yang berada di Indonesia. Meningkatkan Keterampilan Menulis Karangan Deskripsi Siswa Sd Pada Mata Pelajaran Bahasa Indonesia”. Dalam pembelajaran menulis karangan Deskripsi diperlukan model pembelajaran. Model pembelajaran merupakan cara untuk menggunakan sumber belajar dalam proses pembelajaran siswa aktif dan tujuan pembelajaran dapat tercapai. Sehingga hasil belajar siswa dapat meningkat. Oleh karena itu model pembelajaran sangat berpengaruh dalam kegiatan pembelajaran, terutama pada pembelajaran menulis karangan deskripsi. Dengan menggunakan model pembelajaran Think Talk Write (TTW) maka siswa diyakini dapat meningatkan keterampilan menulis bahasa Indonesia. Dan hasil yang diharpakan bagi siswa ialah : 1) Siswa mampu menyesuaikan judul, pengembangan gagasan yang cermat, banyak fakta pendukung, sesuai dengan topik karangan. 2) Siswa mampu Mengorganisasi isi sesuai dengan gagasan pokok, keseluruhan susunan kalimat jelas, urutan logis, kohesi tinggi. 3) Siswa dapat menyusun tata bahasa kompleks dan efektif. 4) Siswa mampu dalam Penggunaan dan pemilihan kata yang efektif, pemilihan kata yang tepat. 5) Siswa mampu menguasai EYD, menguasai tanda baca, dan menguasai kaidah penulisan. Selain itu siswa juga mendapatkan pengalaman belajar yang begitu menarik dan berkesan melalui model pembelajaran Think Talk Write.
PERANAN MODEL THINK TALK WRITE (TTW) DALAM MENINGKATKAN HASIL BELAJAR PADA MATA PELAJARAN PKn MATERI HAK & KEWAJIBAN TERHADAP LINGKUNGAN SISWA SEKOLAH DASAR
Authors: Pramintari Ratna Duhita , Triyanih Sopiyah
Published: June 2023

Pendidikan PKn sangat penting dalam rangka mencerdaskan kehidupan bangsa, sehingga keberhasilan pembelajaran PKn diukur dari tercapainya tujuan pembelajaran PKn. Penelitian ini bertujuan untuk meningkatkan hasil belajar PKn siswa sekolah dasar dengan menggunakan model Think Talk Write. Penelitian ini menggunakan beberapa jurnal ilmiah yang menjadi pedomannya. Pengumpulan data dalam penelitian ini menggunakan jurnal referensi, data-data atau berita dari internet serta koleksi jurnal nasional dan internasional. Penulis menggunakan model Think Talk Write sebagai solusi untuk melatih keterampilan peserta didik mengomunikasikan hasil pemikirannya. Sehingga dalam pembelajaran PKn membuat siswa menjadi aktif, kreatif, inovatif dan menyenangkan. Karena keberhasilan belajar merupakan hal yang sangat penting untuk dijadikan tolak ukur dalam melaksanakan proses belajar mengajar. Dengan menggunakan model Think Talk Write diharapkan siswa dapat meningkatkan hasil belajar PKn pada aspek kognitif, afektif dan psikomotor.
Nature on the airwaves: natural history and the BBC in interwar Britain, 1922–1939 (William T. Stearn Student Essay Prize 2021)
Authors: Long Max
Published: June 2023

Archival and other historical evidence, including digitized issues of The Radio Times and files from the British Broadcasting Corporation’s Written Archives Centre, reveal the pivotal importance that natural history played in the BBC’s programming during the 1920s and 1930s. Beyond merely replicating the forms and styles that could be found across other forms of mass media, natural history broadcasts demonstrated to the British public what radio technology was capable of. At the same time, radio was not isolated from the media landscape of the period, and natural history broadcasts made full use of visual accompaniments such as magazines and pamphlets. Examining a variety of different broadcasts, this paper focuses on how expertise was fashioned behind the microphone, on “stunts” such as broadcasting the live sounds of animals, on audience participation, and on broadcasts aimed at young audiences. Broadcast lessons for schools, which are generally overlooked by historians, offer a rich opportunity for examining how natural history radio developed over the years. Children’s hour characters like the “Zoo Man” modelled an intimate, direct way of engaging with young audiences. Moreover, experiments in field ecology involving listeners in the collection of data showed that radio could act as a two-way medium. These broadcasts helped to shape the modern phenomenon of the nature documentary that would take off after 1945. While the origins of this phenomenon are usually traced either to the establishment of the BBC’s Natural History Unit in 1957, or in some cases to early nature films, there is a strong case for considering the BBC’s interwar broadcasts (1922–1939) as part of this trajectory.
George Montagu (1753–1815): travels in Scotland and his Scottish bird specimens
Authors: Mearns Richard , Mearns Barbara
Published: June 2023

George Montagu (1753–1815) is remembered particularly for his Ornithological dictionary (1802), Testacea Britannica (1803) and his Supplement to the Ornithological dictionary (1813), the works which helped establish his reputation as an astute and competent naturalist. His studies were mainly carried out in southern England, particularly in Wiltshire and Devon, but he travelled widely in Britain and Ireland during his military career. Montagu himself recorded that he had been at Dumbarton, Inverness, Loch Lomond and Mull, but his activities in Scotland in the early 1770s and early 1780s are otherwise not well recorded, there being no dates attached to his observations or specimens. An examination of the 15th Regiment of Foot’s muster rolls has shed some light upon his movements and helps clarify certain aspects of his life, including his elopement. Montagu’s Scottish bird specimens, whether he shot them himself or acquired them from other sources, are of special significance as they are amongst the oldest held in the Natural History Museum at Tring.
Solomon Col Adol (1909–1971), Game Ranger and animal collector in Bor, South Sudan
Authors: Tuttle Brendan
Published: June 2023

The study of fauna in South Sudan during the first two-thirds of the twentieth century relied on a web of collection stations, a vast field network of observers, the assembly of many disparate facts, and the collection of animals to be sold for display and study in zoos and museums in Sudan and other countries. This work of zoological study and the trade in animals mobilized great numbers of South Sudanese, relying on their labour, observations, and knowledge about how to capture and care for animals. These participants have rarely been credited for their own contributions to knowledge about the natural history of their country. This paper examines the work of Solomon Col Adol who was based at the zoological collection station in Bor, a small town on the White Nile, where he was a Game Ranger.
Notes on the birds collected by Giovanni Emilio Cerruti during his journey to New Guinea (1869–1870)
Authors: Ghiraldi Luca , Ruzzon Matteo , Coloberti Marta , Di Meo Alessandro
Published: June 2023

In the later decades of the nineteenth century, some Italian explorers carried out expeditions to the Indonesian archipelago and New Guinea, with the aims of concluding diplomatic agreements, strengthening commercial networks and obtaining territorial concessions. During these expeditions, collecting animal specimens and artefacts, later deposited in several museums, was fairly common, increasing the scientific renown of post-unitarian Italy. Giovanni Emilio Cerruti ( fl. 1860–1875) was a traveller, little known today, who visited those then remote lands. He was a merchant and a strong supporter of colonial policies. Through the mediation of government officials, he managed to obtain a commission from the Italian government to identify one or more places close to New Guinea where a penal colony could be established. Although the trip was commissioned exclusively for diplomatic purposes, Cerruti collected some interesting ornithological specimens, which were later donated to Italian natural history museums. We reconstruct Emilio Cerruti’s journey, using his letters to illuminate the most interesting details of its natural history. Fifty of Cerruti’s specimens, extant in the collections of the Museo di Scienze Naturali di Torino and Museo Calderini di Varallo Sesia, are recorded in an annotated catalogue.
Daily Activity Restriction Quantification in Older Population via Using Activity Frequency: Dual Roles in Preventing Falls
Authors: Shen Xia , Chen Lin Yu , Wang Jing Xin , Jiang Yan Na
Published: June 2023

Daily activity restriction is an expected physical behavior aiming to prevent falls in the older population. However, it plays dual roles in preventing falls, both positive and harmful. Therefore, the degree of daily activity restriction is proposed as a critical factor influencing the weight ratio of the positive and negative roles and finally determining the efficacy of fall prevention. Thus, quantification of daily activity restriction is essential to learn its efficacy.

We proposed using activity frequency to quantify activity restriction and have testified its good sensitivity to discriminate the degree of activity restriction in older populations. We further attempted to link the degree of activity restriction with the degree of fear of falling to estimate the dual roles of activity restriction in preventing falls. Based on it, a new Composite Activity-specific Risk of Falls Scale tool has been developed. It is promising to use for guiding the modification of physical behavior to prevent falls in older populations. However, further studies are required to establish the evidence for its application.
Late Decrease in Cerebral Blood Flow in Bacterial Meningitis: More than a Simple Normalization of Acute Inflammatory Vessel Wall Architecture?
Authors: Kumar Vivig Shantha , Kumar Vignarth Shantha
Published: June 2023

Acute bacterial meningitis is a disease with an overwhelmingly high mortality rate and high incidence of adverse neurological sequelae and poor neurological recovery amongst survivors. Amongst the numerous complications of bacterial meningitis, the presence of cerebrovascular disease represents a severe disease form. Vascular involvement during bacterial meningitis has long been established by numerous pathological and angiographic studies. Cerebrovascular changes known to occur in bacterial meningitis ranging from narrowing of large arteries by vasospasm to critical stenosis/obliteration of small to medium sized arteries/arterioles by vasculitis. Not surprisingly, alterations in CBF velocities have commonly been described during the inflammatory process and may represent an important component of brain injury during meningitis. In accordance with previous studies observing a biphasic cerebral flow pattern characterized by an early but transient increase in flow velocity, mostly due to reflexive vasospasm, and later by a sustained decrease in flow velocity, likely attributable to stenotic vasculitis, cerebral ischemia is a notable complication of bacterial meningitis during the advanced disease phase. Impaired cerebral perfusion during the late stages of disease may result from a variety of factors that contribute to a vital component of cerebral injury in bacterial meningitis. The pathogenesis of cerebral ischemia with progression of disease course is less clearly understood but may involve a complex interaction between inflammatory processes, systemic dysfunction, energy impairment, neuronal damage and intracranial pressure, factors of which we aim to more precisely understand and assign a more definite contributory role in the development of cerebrovascular ischemic consequences with advanced stages of bacterial meningitis.
Being at-promise girls in the 2020s
Authors: Peng Lance
Published: June 2023

This 15-minute talk examines contextual and sociocultural factors that contribute to the development of adolescent girls' agency, with a focus on the key components of participation, authority, and deliverance. I offer insights into the ways in which cultural, political, and social trends impact the development of adolescent girls' agency in the 2020s. The talk also explores recent global activities and milestones that have impacted girls' development, with a particular focus on the rise of cyberactivism and the effects of COVID-19. Using personal experience working with justice-involved girls in out-of-home placements, I share observations of girls at-promise against the backdrop of the prevailing cultural, political, and social matrix. Through this analysis, the presentation provides a thematic frame of reference for those involved in adolescent female development and highlights the key transformative cultural dynamics that need to be considered in order to promote the development of adolescent girls' agency.
Effects of Melatonin Administration on Vasomotor Activity and Histological Structure of Isolated Thoracic Aorta in Rats Treated with Thyroxine
Authors: ÜSTÜNDAĞ Hilal , ŞENTÜRK Esra , YILDIRIM Serkan , ÇELEBİ Fikret , GÜL Mustafa
Published: June 2023

Objective: The goal of this study was to examine the effect of in vivo melatonin (MEL) administration on isolated thoracic aorta in rats with thyroxine treatment and its duty in aortic response to contractile agents, such as potassium chloride (KCl) and phenylephrine (PE). In addition, immunohistological alterations were also examined.

Methods: Experimental groups were as follows: control group (n= 5), thyroxine group (n= 5), melatonin group (n= 6), and thyroxine + melatonin group (n= 6). L-thyroxine was given by intraperitoneal (i.p) administration at 0.3 mg/kg/day for 14 days. MEL was administered i.p., at 3 mg/kg/day for 14 days. The thoracic aorta was isolated from rats euthanized by cervical dislocation. Then, vascular rings were prepared.

Concentration-response curves for KCl and PE applications were recorded in an isolated organ bath. Tissue samples were fixed in 10% formalin for histopathological and immunohistological evaluation.

Results: KCl and PE-induced contractions were reduced significantly in the thoracic aortic rings of the thyroxine-treated rats. MEL administration partially attenuated the reduction in the contraction responses due to thyroxine treatment. Immunohistological findings showed that MEL inhibits the thickening of the vessel wall by probably suppressing collagen formation due to thyroxine treatment in the aortic tissue.

Conclusion: Our results suggest that MEL may attenuate the decrease in vascular resistance caused by thyroxine treatment.
Conhecimentos acerca da doença de Chagas por alunos do ensino médio de uma escola particular de ensino em Belo Horizonte, Minas Gerais.
Authors: Brandão Lucas de Esquivel Dias , Nascimento Juliana Macedo Lacerda , Barros Marcelo Diniz Monteiro de
Published: June 2023

Globalmente, a doença de Chagas está associada a 14 mil mortes anuais, constituindo-se na sexta doença tropical de maior importância no mundo. Na América Latina pode ser entendida como uma das mais importantes doenças parasitárias, em que mais de 10 milhões de pessoas são infectadas com o parasito Trypanosoma cruzi, e aproximadamente 90 milhões de indiví­duos ainda estão sob o risco de contraí­rem a doença. No Brasil é responsável por cerca de 6 mil mortes anuais (16 por dia). A Fundação Oswaldo Cruz (Fiocruz) estima que existam de 2 a 3 milhões de pessoas com a forma crônica no paí­s. Sendo assim, trabalhos sobre o conhecimento da população acerca dessa zoonose são essenciais para verificar como o assunto vem sendo tratado na sociedade atual. No primeiro semestre de 2015 foi realizada uma investigação de cunho descritivo com 21 alunos, da faixa etária de 16 e 17 anos, do 2º ano do Ensino Médio em uma escola particular de ensino, localizada em Belo Horizonte, Minas Gerais. Através de um questionário semiestruturado, contendo 15 questões, almejou-se identificar que conhecimentos esses alunos tinham sobre a Doença de Chagas. Na presente pesquisa 85,71% dos alunos responderam conhecer sobre a Doença de Chagas na escola e apenas 9,52% relataram que esses conhecimentos foram adquiridos através de jornais e noticiários, evidenciando, portanto, que tais meios de comunicação não vigoraram como as principais fontes de obtenção de conhecimentos por parte dos alunos. Registrou-se, ainda, que a maioria dos estudantes não conhecem pessoas que desenvolveram a Doença de Chagas, o que em parte pode comprovar o fato de que a transmissão desta doença encontra-se controlada no Estado de Minas Gerais. Para avaliações futuras julgamos que pesquisas qualitativas sobre a percepção da população sobre o tema contribuiriam enormemente para o planejamento de ações educativas acerca dessa importante zoonose.
A entrada na carreira docente de professoras egressas do curso de Pedagogia da Universidade Estadual de Mato Grosso do Sul (UEMS), unidade de Paranaí­ba-MS
Authors: Martins Thaís Regina Miranda , Slavez Milka Helena Carrilho
Published: June 2023

O tema abordado está voltado aos desafios de professoras no iní­cio da carreira docente. O ingresso na docência requer um olhar diferenciado, pois nesta fase, os professores podem passar por diversas experiências que muitas vezes são dificultosas, sendo marcadas por medos, incertezas e angústias. Desse modo, esta pesquisa tem como objetivo geral verificar as dificuldades e contribuições encontradas na entrada da carreira docente de doze professoras egressas do curso de Pedagogia da Universidade Estadual de Mato Grosso do Sul (UEMS), unidade de Paranaí­ba-MS. Os objetivos especí­ficos foram conhecer os problemas percebidos no iní­cio da profissão de professor, identificar os problemas voltados à prática docente em iní­cio de carreira, reconhecer problemas que envolvem a socialização e apontar contribuições da formação inicial para superação das dificuldades das professoras iniciantes. A investigação apoiou-se em estudos de Veenman (1988), tratando sobre dificuldades da profissão de professor e Huberman (1992), tratando sobre o ciclo de vida profissional do professor. A metodologia utilizada tem enfoque qualitativo e os procedimentos empregados para o desenvolvimento da pesquisa são questionários semiestruturados. Alguns resultados apontaram que as maiores dificuldades enfrentadas em sala de aula no iní­cio da carreira referem-se à indisciplina dos alunos e falta de recursos materiais para o trabalho pedagógico. Revela ainda um número significativo de egressos que conseguem estabelecer relações de aspectos teóricos que fizeram parte da formação acadêmica e um dos principais aspectos que acreditam ter contribuí­do significativamente para a formação profissional docente foi o estágio supervisionado.
Palavras-chave: Professoras iniciantes, Pedagogia, Carreira docente, Dificuldades.
Sociedade da transparência de Byung-Chul Han: a pornografia enquanto conceito filosófico
Authors: Noyama Samon , Costa Pâmela bueno
Published: June 2023

Neste livro o filósofo sul coreano Byung-Chul Han nos apresenta a ideia de transparência como um conceito imprescindí­vel para compreender os fenômenos sociais e cibernéticos que consolidam a sociedade contemporanea como um regime de controle, cujo reflexo imediato é a cultura da exibição, da desritualização e da nudez. Contudo, para Han, essa exibição exacerbada que se transformou no modo de ser e estar do homem contemporâneo, que se oferece na forma da transparência, sugerindo algo benéfico e importante, constitui efetivamente uma sociedade pornográfica, que nada tem de sedutora, construitiva ou reflexiva.
Gêneros de texto por meio de práticas sociais em lí­ngua inglesa: o papel da sequência didática produzida por pibidianos
Authors: Siebeneicher Brito Karim , Percinoto Poliseli Corrêa Francini , Rodrigues Tognato Maria Izabel
Published: June 2023

Neste artigo, apresentamos os resultados da análise de uma sequência didática (SD) desenvolvida por integrantes do subprojeto de lí­ngua inglesa do PIBID da Universidade Estadual do Paraná – Campus de União da Vitória, contemplando o gênero de texto Cardápio. Baseamo-nos, para tanto, na relação entre o ensino com base em gêneros e o desenvolvimento das capacidades de linguagem (BRONCKART, 1997/2009; SCHNEUWLY; DOLZ, 2004), o que permite aos alunos agir fazendo uso da linguagem em diferentes situações de comunicação. No que concerne aos procedimentos metodológicos deste estudo, quanto aos instrumentos de coleta de dados, realizamos a análise de uma sequência didática. No que tange aos procedimentos de análise, pautamos nossos estudos na identificação das capacidades de linguagem mobilizadas nessa sequência. Os resultados deste estudo apontam para a relevância da inserção de professores em formação em processos de produção de materiais didáticos para a tomada de consciência dos processos de regulação da linguagem e para possí­veis contribuições quanto a uma atuação profissional mais precisa e segura.
A domestic rooftop PV system: a step towards retrofitting the built environment to combat climate change in Bahrain
Authors: Alnaser Naser W.
Published: June 2023

This article assesses the technological, economic, and environmental aspects of installing the first 7.8 kW PV installation on a rooftop of a domestic house in Bahrain for a period of 2 years (20th March 2018 to 31st March 2020). The maximum solar electricity generated was 1,228.9 kWh (August 2018), and the least was 728.16 kWh (December 2019). The maximum daily specific yield (SY) was 6.12 kWh/kWp (on 14 April 2019). The annual average daily specific yield for this domestic building was 4.13 kWh/kWp. The average performance ratio (PR) of the PV system was 73.0% in 2019. The self-sufficiency (SS) of this installation was found to vary from 15.3% to 50.7%. The average SS value in 2018 was 22.8%, while in 2019 it was 28.6%. Furthermore, a cubic relation correlation was established, relating the month number (X) and the monthly average of the daily specific yield (Y). It was found that installing a solar PV system will cut about 39.0% of CO2 annually, which is equal to 4.637 tons and hence saves 38,567 ft3 of natural gas. The initiative of encouraging the use of rooftops in Bahrain to produce zero-carbon electricity is a step towards retrofitting the built environment to combat climate change.
Identification of natural killer markers associated with fatal outcome in COVID-19 patients
Authors: Tarantino Nadine , Litvinova Elena , Samri Assia , Soulié Cathia , Morin Véronique , Rousseau Alice , Dorgham Karim , Parizot Christophe , Bonduelle Olivia , Beurton Alexandra , Miyara Makoto , Ghillani Pascale , Mayaux Julien , Lhote Raphael , Lacorte Jean-Marc , Marcelin Anne-Geneviève , Amoura Zahir , Luyt Charles-Edouard , Gorochov Guy , Guihot Amélie , Vieillard Vincent
Published: June 2023

Introduction

Increasing evidence has shown that coronavirus disease 19 (COVID-19) severity is driven by a dysregulated immunological response. Previous studies have demonstrated that natural killer (NK) cell dysfunction underpins severe illness in COVID-19 patients, but have lacked an in-depth analysis of NK cell markers as a driver of death in the most critically ill patients.

Methods

We enrolled 50 non-vaccinated hospitalized patients infected with the initial virus or the alpha variant of SARS-CoV-2 with moderate or severe illness, to evaluate phenotypic and functional features of NK cells.

Results

Here, we show that, consistent with previous studies, evolution NK cells from COVID-19 patients are more activated, with the decreased activation of natural cytotoxicity receptors and impaired cytotoxicity and IFN-γ production, in association with disease regardless of the SARS-CoV-2 strain. Fatality was observed in 6 of 17 patients with severe disease; NK cells from all of these patients displayed a peculiar phenotype of an activated memory-like phenotype associated with massive TNF-α production.

Discussion

These data suggest that fatal COVID-19 infection is driven by an uncoordinated inflammatory response in part mediated by a specific subset of activated NK cells.
Human-umbilical cord matrix mesenchymal cells improved left ventricular contractility independently of infarct size in swine myocardial infarction with reperfusion
Authors: Raposo Luís , Cerqueira Rui J. , Leite Sara , Moreira-Costa Liliana , Laundos Tiago L. , Miranda Joana O. , Mendes-Ferreira Pedro , Coelho João Almeida , Gomes Rita N. , Pinto-do-Ó Perpétua , Nascimento Diana S. , Lourenço André P. , Cardim Nuno , Leite-Moreira Adelino
Published: June 2023

Background

Human umbilical cord matrix-mesenchymal stromal cells (hUCM-MSC) have demonstrated beneficial effects in experimental acute myocardial infarction (AMI). Reperfusion injury hampers myocardial recovery in a clinical setting and its management is an unmet need. We investigated the efficacy of intracoronary (IC) delivery of xenogeneic hUCM-MSC as reperfusion-adjuvant therapy in a translational model of AMI in swine.

Methods

In a placebo-controlled trial, pot-belied pigs were randomly assigned to a sham-control group (vehicle-injection; n = 8), AMI + vehicle (n = 12) or AMI + IC-injection (n = 11) of 5 × 105 hUCM-MSC/Kg, within 30 min of reperfusion. AMI was created percutaneously by balloon occlusion of the mid-LAD. Left-ventricular function was blindly evaluated at 8-weeks by invasive pressure-volume loop analysis (primary endpoint). Mechanistic readouts included histology, strength-length relationship in skinned cardiomyocytes and gene expression analysis by RNA-sequencing.

Results

As compared to vehicle, hUCM-MSC enhanced systolic function as shown by higher ejection fraction (65 ± 6% vs. 43 ± 4%; p = 0.0048), cardiac index (4.1 ± 0.4 vs. 3.1 ± 0.2 L/min/m2; p = 0.0378), preload recruitable stroke work (75 ± 13 vs. 36 ± 4 mmHg; p = 0.0256) and end-systolic elastance (2.8 ± 0.7 vs. 2.1 ± 0.4 mmHg*m2/ml; p = 0.0663). Infarct size was non-significantly lower in cell-treated animals (13.7 ± 2.2% vs. 15.9 ± 2.7%; Δ = −2.2%; p = 0.23), as was interstitial fibrosis and cardiomyocyte hypertrophy in the remote myocardium. Sarcomere active tension improved, and genes related to extracellular matrix remodelling (including MMP9, TIMP1 and PAI1), collagen fibril organization and glycosaminoglycan biosynthesis were downregulated in animals treated with hUCM-MSC.

Conclusion

Intracoronary transfer of xenogeneic hUCM-MSC shortly after reperfusion improved left-ventricular systolic function, which could not be explained by the observed extent of infarct size reduction alone. Combined contributions of favourable modification of myocardial interstitial fibrosis, matrix remodelling and enhanced cardiomyocyte contractility in the remote myocardium may provide mechanistic insight for the biological effect.
Structural architecture and late Cenozoic tectonic evolution of the Ulsan Fault Zone, SE Korea: New insights from integration of geological and geophysical data
Authors: Cheon Youngbeom , Shin Young Hong , Park Samgyu , Choi Jin-Hyuck , Kim Dong-Eun , Ko Kyoungtae , Ryoo Chung-Ryul , Kim Young-Seog , Son Moon
Published: June 2023

Integration of geological and geophysical data is essential to elucidate the configuration and geometry of surface and subsurface structures, as well as their long-term evolution. The NNW–SSE-striking incised valley and parallel mountain range in the southeastern margin of the Korean Peninsula, extending 50 km from Gyeongju to Ulsan cities, are together regarded as one of the most prominent geographical features in South Korea. This paper presents an investigation into the structural architecture and deformation history of the valley and mountain range during the late Cenozoic based on combined data from field observations and gravity and electrical resistivity surveys. Our results based on integrated and reconciled geological, structural, and geophysical data are as follows. First, the incised fault valley can be divided into 1) the northern part, which comprises several distributed buried or exposed fault strands; and 2) the southern part, which comprises a concentrated deformation zone along the eastern margin of the valley. Different deformation features between the two parts are controlled by the lithology of host rocks and by the location and geometry of the neighboring major structures, that is, the Yeonil Tectonic Line (YTL) and the Yangsan Fault. Second, we defined the Ulsan Fault Zone as a NNW–SSE-to N–S-striking fault within the incised valley and along the eastern margin of the valley. In particular, the constituent strands located along the eastern margin of the valley have acted mainly as an imbricate thrust zone, characterized by an east-side-up geometry with moderate to low dip angles and reverse-dominant kinematics in the shallow subsurface during the Quaternary. Third, reactivated strands within the Ulsan Fault Zone during the Quaternary are interpreted as shortcut faults developed in the footwall of Miocene subvertical structures, predominantly the YTL. In addition, movements on the Ulsan Fault Zone and the YTL during the Miocene to Quaternary were arrested by the NNE–SSW-striking Yangsan Fault, which was a prominent and mature pre-existing structure. Our results highlight the spatiotemporal structural variation in SE Korea and emphasize the strong control of the configuration and geometry of pre-existing structures on the distribution and characteristics (i.e., geometry and kinematics) of the subsequent deformation under changing tectonic environments through the late Cenozoic.
Local Moho distribution in the eastern Mediterranean region from gravity inversion: eastern Mediterranean Sea
Authors: Harash Fayez , Khalaf Amin , Al-Ansari Nadhir , Chen Chao , Alrawi Imad , Al-Khafaji Harith , Tageldin Mohammed
Published: June 2023

This study investigates the variations of surface Moho inverted from Bouguer gravity data beneath the northwestern Arabian plate. The results were obtained by applying the 3DINVER program developed in a MATLAB environment using the Parker–Oldenburg method. The calculation results show that the maximum Moho depth observed in the western Arabian shield approaches more than 40 km, while the minimum values of 16–20 km are mainly concentrated beneath the Mediterranean Sea. A key innovation of this study is its implementation of exponential density contrast decrease in the sediment–basement interface in the Syrian part of the Arabian plate. The gravity anomaly of the sediment is computed using a density contrast which varies exponentially with depth, leading to a more accurate representation of the subsurface structure and offering valuable insights into the geodynamic processes and seismogenic potential of the area. To validate the inverted results from the 3DINVER program, the study compares these findings with seismic results that show good agreement with our results from the same region. This leads to a better understanding of the crustal structure and provides insights into its implications for the geodynamic and seismogenic nature of the northwestern Arabian plate—critical for assessing the potential for earthquakes and other geological hazards. The density contrast between the Earth’s crust and mantle can be related to variations in the composition, temperature, and pressure of the rocks. These factors influence the mechanical properties of the crust, ultimately affecting the tectonic processes and seismic activity in the region.
Contextualizing the Mathematical Knowledge for Teaching Framework for teachers of Emergent Bilinguals
Authors: Fernández Luis Miguel , Ortiz Galarza Mayra
Published: June 2023

We are amid a rapid demographic shift, with Emergent Bilinguals (EBs) being the fastest growing K-12 student population. This has created an ambitious goal for teacher education programs as they must prepare mathematics pre-service teachers (PSTs) to assess the needs of EBs. As a response, this study conducted a qualitative analysis of 16 PSTs to propose a contextualized version of the Mathematical Knowledge for Teaching (MKT) framework as an emerging knowledge base of teaching that can be used to further guide the planning and enactment of teacher education programs in the mathematics education of EBs, specifically.
Does environmental information disclosure drive corporate sustainable growth? A new insight into U-shaped relationship
Authors: Zhang Jinsong , Wang Mengmeng
Published: June 2023

Due to the increasing rate of economic development and the increasingly serious problem of environmental degradation, environmental information disclosure has become an important basis for promoting carbon peaking and carbon neutrality, and an important way for enterprises to carry out green governance to achieve sustainable development. This study uses empirical research methods to analyze the relationship between environmental information disclosure and corporate sustainable growth in the context of green governance using panel data of Chinese A-share listed companies in Shanghai and Shenzhen from 2012 to 2021. The empirical tests conclude that there is a U-shaped relationship between environmental information disclosure and corporate sustainable growth, which decreases and then increases, and the U-shaped relationship is transmitted through innovation inputs. The U-shaped relationship between environmental information disclosure and corporate sustainable growth is weakened by firm size and enhanced by equity incentives. In addition, further group analysis reveals that the above U-shaped relationship is more significant in non-state enterprises than in state-owned enterprises.
Amide-containing neoepitopes: the key factor in the preparation of hapten-specific antibodies and a strategy to overcome
Authors: Han Xiangning , Lin Hong , Chen Xiangfeng , Wang Luefeng , Zhang Ziang , Wei Xiaojing , Sun Xun , Xie Hanyi , Pavase Tushar Ramesh , Cao Limin , Sui Jianxin
Published: June 2023

For a long time, people have suffered from uncertainty, complexity, and a low success rate in generating and screening antibodies against small molecules, which have become the core bottlenecks of immunochemistry. Here, the influence of antigen preparation on antibody generation was investigated at both molecular and submolecular levels. Neoepitopes (amide-containing neoepitopes) formed in the preparation of complete antigens are one of the most important factors limiting the efficiency of generating hapten-specific antibodies, which was verified by different haptens, carrier proteins, and conjugation conditions. Amide-containing neoepitopes present electron-dense structural components on the surface of prepared complete antigens and, therefore, induce the generation of the corresponding antibody with much higher efficiency than target hapten. Crosslinkers should be carefully selected and not overdosed. According to these results, some misconceptions in the conventional anti-hapten antibody production were clarified and corrected. By controlling the content of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) during the synthesis of immunogen to limit the formation of amide-containing neoepitopes, the efficiency of hapten-specific antibody generation could be significantly improved, which verified the correctness of the conclusion and provided an efficient strategy for antibody preparation. The result of the work is of scientific significance in the preparation of high-quality antibodies against small molecules.