bluuebunny/crossref_metadata_2025 huggingface dataset:
The dynamism of communication technology adoption, market information and welfare: Evidence from Nile perch (Lates niloticus) fish market, Mwanza, Tanzania
Authors: Kitole Felician , Lihawa Robert , Sesabo Jennifer , Shitima Christina
Published: May 2023

Despite the growing importance of digital technology in modern society, its adoption in some sectors and regions has been slow. One such sector is fishing in developing countries, where the potential benefits of technology have yet to be fully realized. This study examines the adoption of communication technology among Nile perch fishers on Lake Victoria in Mwanza, Tanzania. Using a Probit model and Ordinary Least Square (OLS) estimations with Robust Standard Errors (OLSR), the study analyzes the impact of technology adoption on fishers' welfare, with a focus on income. The results suggest that the type of mobile phone, fishing experience, and the desire to exploit digital marketing are key factors in technology adoption among fishers. Furthermore, the study finds that technology adoption has a significant positive impact on fishers' welfare, indicating that digitalization can increase market competitiveness, openness, and improve livelihoods. To capitalize on these benefits, the study recommends that the government should support the digitalization of the fishing market and make accurate and timely market information readily available to fishers.
The neighbouring genes AvrLm10A and AvrLm10B are part of a large multigene family of cooperating effector genes conserved in Dothideomycetes and Sordariomycetes
Authors: Talbi Nacera , Fokkens Like , Audran Corinne , Petit‐Houdenot Yohann , Pouzet Cécile , Blaise Françoise , Gay Elise J. , Rouxel Thierry , Balesdent Marie‐Hélène , Rep Martijn , Fudal Isabelle
Published: May 2023

Fungal effectors (small‐secreted proteins) have long been considered as species or even subpopulation‐specific. The increasing availability of high‐quality fungal genomes and annotations has allowed the identification of trans‐species or trans‐genera families of effectors. Two avirulence effectors, AvrLm10A and AvrLm10B, of Leptosphaeria maculans, the fungus causing stem canker of oilseed rape, are members of such a large family of effectors. AvrLm10A and AvrLm10B are neighbouring genes, organized in divergent transcriptional orientation. Sequence searches within the L. maculans genome showed that AvrLm10A/AvrLm10B belong to a multigene family comprising five pairs of genes with a similar tail‐to‐tail organization. The two genes, in a pair, always had the same expression pattern and two expression profiles were distinguished, associated with the biotrophic colonization of cotyledons and/or petioles and stems. Of the two protein pairs further investigated, AvrLm10A_like1/AvrLm10B_like1 and AvrLm10A_like2/AvrLm10B_like2, the second one had the ability to physically interact, similarly to what was previously described for the AvrLm10A/AvrLm10B pair, and cross‐interactions were also detected for two pairs. AvrLm10A homologues were identified in more than 30 Dothideomycete and Sordariomycete plant‐pathogenic fungi. One of them, SIX5, is an effector from Fusarium oxysporum f. sp. lycopersici physically interacting with the avirulence effector Avr2. We found that AvrLm10A/SIX5 homologues were associated with at least eight distinct putative effector families, suggesting that AvrLm10A/SIX5 is able to cooperate with different effectors. These results point to a general role of the AvrLm10A/SIX5 proteins as “cooperating proteins”, able to interact with diverse families of effectors whose encoding gene is co‐regulated with the neighbouring AvrLm10A homologue.
Feminist Gothic: Anonymity and intersectionality of oppression in Eva Figes's The Tenancy
Authors: Xu Lian
Published: May 2023

The Gothic genre has historically been adopted by women writers to express their subversive gender views. In The Tenancy, Figes depicts the systematic oppression of the patriarchal society against women during the latter half of the twentieth century. She also revises the traditional Gothic topos of “oppression” in terms of three aspects: victim, intersectionality, and the source of oppression. Female victims no longer hold traditional gender values; they are new women who are oppressed for their nonconformity. This novel also shows that, with the development of technology and urbanization, patriarchal oppression has become more anonymous and more systematic, during which process gender and class parameters intersect with each other. Figes's revision upon the “oppression” topos reveals a more revolutionary insight than most Gothic novels in the eighteenth and nineteenth centuries. As a result, The Tenancy marks a significant transition from the female Gothic to the feminist Gothic.
A novel catheter ablation strategy for non‐paroxysmal atrial fibrillation combining cryoballoon, radiofrequency, and Marshall‐vein ethanol ablations
Authors: Hayasaka Kazuto , Sasaki Takeshi , Shirai Yasuhiro , Shimosato Hikaru , Tahara Tomohiro , Segami Shota , Nagasawa Ryo , Akimoto Ko , Yabe Kento , Toya Chisashi , Watanabe Keita , Yamashita Shu , Suzuki Masahito , Sugiyama Koji , Yamauchi Yasuteru , Okishige Kaoru , Goya Masahiko , Sasano Tetsuo
Published: May 2023

Backgrounds

Catheter ablation for non‐paroxysmal atrial fibrillation (non‐PAF) remains challenging and more effective strategy has been required to reduce postoperative arrhythmia recurrences. This study aims to investigate the efficacy and safety of a novel extensive ablation strategy for non‐PAF, that is based on a combination of cryoballoon (CBA), radiofrequency (RFA), and Marshall‐vein ethanol ablations (EA‐VOM).

Methods

The study was a single‐center, retrospective observational study. We enrolled 171 consecutive patients who underwent de‐novo catheter ablation for non‐PAF under conscious sedation with a novel extensive ablation strategy that included CBA for pulmonary vein isolation (PVI) and left atrial roof ablation (LARA), RFA for mitral isthmus (MI) ablation, superior vena cava isolation, and other linear ablations and EA‐VOM. Recurrence of atrial arrhythmias over 1 year, procedure outcomes, and procedure‐related complications were investigated.

Results

A total of 139 (81.3%) patients remained in sinus rhythm during 1‐year follow‐up. Of the 139 patients, 51 patients (29.8%) received antiarrhythmic drugs. The mean procedure time was 204 ± 45 min. PVI and LARA ablation by CBA and MI block by RFA and EA‐VOM were completed in 171 (100%) and 166 (97.1%) patients, respectively. No serious procedure‐related complications were observed except for one case of delayed pericardial effusion.

Conclusion

Approximately 80% of the study patients were AF‐free during 1‐year follow‐up period after a single procedure based on the novel extensive ablation strategy combining CBA, RFA, and EA‐VOM. This strategy for non‐PAF may be preferred in terms of maintenance of sinus rhythm, safety even in high‐risk patients, and relatively short procedure time.
Feeling insecure and excluding immigrants: Relationship between subjective risks and welfare chauvinism
Authors: Duman Anil
Published: May 2023

We argue that subjective insecurity plays an important role in explaining welfare chauvinism, which is defined as the restriction of immigrants' access to social benefits and public services. Additionally, macroeconomic performance and welfare regime are closely related to opinions about the social rights of migrant groups. We test these propositions, using a multilevel ordered logit model using the 8th wave of ESS. It is found that subjective unemployment and income risks are not overlapping with objective measures, and self‐assessed insecurity has a strong and positive effect on welfare chauvinism. Moreover, we demonstrate that, even for the most socio‐economically advantaged respondents, subjective risk increases the likelihood of chauvinistic welfare attitudes. At the macro level, higher rates of GDP per capita growth decrease welfare chauvinism, and Central and Eastern European welfare regime increases the likelihood of exclusionary attitudes in relative terms. The results are robust across different estimation techniques and inclusion of alternative contextual factors.
Relevance of Strategically Planned Engineering Education for Sustainable Development
Authors: Nay ZarAung  , Aye Myint
Published: May 2023

Every nation is paying attention to and strategically improving SDG-4 (Quality Education) itself and using it as a tool for fulfilling other related SDGs. Today, engineering education plays a vital role in implementing a significant portion of SDGs and thus strategic plan for engineering education also becomes important. Although many of existing works have focused on how to implement SDGs through education, there are very few works which study on the relevance of educational strategic plan for SDGs.

The objectives of this paper are to review how higher education, which includes engineering education, is strategically planned in a National Education Strategic Plan and to analyse which strategies support other SDGs in addition to SDG-4. As a case study, the review is conducted for Myanmar NESP (2016-2021). By means of questionnaires, a total of 240 institute/university heads of departments/deans, teachers/lecturers and students from fifteen different universities are surveyed to explore relevance of NESP for other SDGs in addition to SDG-4.

First, according to intuitive analysis, it finds that Myanmar NESP strategies for higher education support SDG-6, SDG-7, SDG-8, SDG-9, SDG-13, SDG-14, SDG-15 and SDG-17 in addition to SDG-4. It is agreed and verified by survey results. The survey results show that in addition to SDG-4, NESP can support SDG-9 in second place, SDG-11 in third place, SDG-6 in fourth place, SDG-7 and SDG-17 in fifth place. More than 95% agree to strategically include the concept of sustainable development in university curriculum. A total of six potential recommendations are made to be considered in developing a NESP in the future. These are (1) to have policy/strategy for inclusion of SD concept as an elementary course in universities, (2) to increase policy/strategy for promoting STI through research, (3) to have policy/strategy for promoting “Education for SD” in teacher education, (4) to increase policy/strategy to promote SD through campus operation, (5) to have policy/strategy for promoting technological entrepreneur (techno-preneur) (6) to have policy/strategy for initiating graduate programs in “Sustainable Development” in universities.
Teaching and Learning Engineering Mathematics by Project Based Learning Method
Authors: Rashmi Rani  , Rajesh Kanna  , Amer Abdelaal  , Durai Pon Raj
Published: May 2023

Teaching Mathematics in higher education is most challenging task for instructors. The objective of this paper is to improve teaching and learning engineering Mathematics via project-based learning. The major intention of this paper is to develop student’s problem-solving skills using projects. This paper describes a method which will increase the interest of student to learn calculus. Students were asked to do a small project to emphasize the application of calculus. The paper emphasizes the importance of linking real time application of engineering calculations with Mathematics. In this paper, method to find surface area of curve rotating about an axis is taught through different theorem and further one curve is chosen to fabricate. This paper will demonstrate substantial difference between normal classroom teaching and project-based learning. This will enhance the teaching process of Engineering Mathematics among the students and faculty members. This paper will also allow the readers to develop new models to link various Mathematical calculations.
Trial through Communication Games for Teamwork Skill Enhancement at First-year Students in Science and Technology Course
Authors: Hiroyuki Matsumoto  , Kenji Amagai  , Yasushi Yuminaka
Published: May 2023

The Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan had been promoted a “program of student employability” to cultivate a sense of sociality and independence in university education. Reflecting these trends, educational programs to improve the teamwork skills are in demand. At our university, first-year students are trained in group work skills and cooperative education. For this purpose, Career Planning and Career Design coursesare offered during first and second semesters of first-year education. Enhancement of teamwork skills at an early stage of education is deemed extremely important. In this paper, we report on a training program to enhance teamwork skills for first-year students of science and technology. Communication games were used to evaluate the importance of communication and teamwork. The effect of this trial was analyzed through a student questionnaire. As a result, weconfirmed that students experienced the importance of teamwork for the solving of problems through the trial of teamwork ability using communication games.
Evaluation of Mathematical Competencies among Electrical Engineering Students
Authors: Mohd Puzi Nur Izrah , Ahmad Azli Naziha , Osman Sharifah , Mohammad Yusof Yudariah
Published: May 2023

In universities, engineering students are often seen as a group of students with high level of intelligence. The engineering programme seeks applications from students with a strong command in Mathematics and Science. Therefore, it is believed that Mathematical Competencies (MC) are the element that plays a vital role in engineering education since there are plenty of applications of mathematical knowledge in engineering courses. This paper discusses mathematical competencies further, with emphasis on those demonstrated by the engineering students. MC consist of eight components: Thinking mathematically, posing and solving a mathematical problem, modelling mathematically, reasoning mathematically, representing mathematically, handling mathematical symbols and formalisms, communicating in, with, and about mathematics and using aids and tools. Unfortunately, many engineering students did not meet the required level as they did not perform well in the engineering course, whereby in this study, the focus was on the Electromagnetic Field Theory (EMT) course. EMT was chosen because the course contains high mathematical component. A qualitative method of ethnographic research design was selected as a mean to collect data, which was then analysed using thematic analysis. A set of EMT questions was developed for 17 participants from an electrical engineering programme. From the written work, it can be said that the participants demonstrated their MC based on their answers. It can be concluded that most of the participants with higher marks were able to demonstrate almost all of the MC components.
Contextual Knowledge Elements Utilization in 3D CAD Model Manipulation from the Practicing Engineers Perspectives
Authors: Adnan Mohd Fahmi , Saud Muhammad Sukri , Daud Mohd Fadzil
Published: May 2023

Engineers’ ability to contextualize their created 3D CAD model during the design stage is the most important aspect of product design. In this conducted research, researcher has found that there was a lack of contextual knowledge among Mechanical Engineering Undergraduates and fresh graduate engineers in utilizing Three-Dimensional Computer Aided Design (3D CAD) modeling software in developing a good product design. In addressing this issue, a study has been conducted to focus on the representation of contextual knowledge elements in 3D CAD modeling applications. This article focused on presenting essential elements of contextual knowledge utilized among practicing engineers in their daily design works, in the aspect of Model Manipulation. Transcendental phenomenology approach has been utilized as the main research methodology. Four practicing engineers from engineering department of one shipbuilding company in Peninsular Malaysia were purposively selected to be studied. From the analysis, there are three most frequent emerging themes in the application of contextual knowledge in manipulating model using 3D CAD modeling: Realization, Design Intention and Normalization. These three elements play an important role in helping engineers to contextualize their design work during the stage of manipulating created model for new product development process.
On the derived category of the Iwahori–Hecke algebra
Authors: Hellmann Eugen
Published: May 2023

We state a conjecture that relates the derived category of smooth representations of a $p$-adic split reductive group with the derived category of (quasi-)coherent sheaves on a stack of L-parameters. We investigate the conjecture in the case of the principal block of ${\rm GL}_n$ by showing that the functor should be given by the derived tensor product with the family of representations interpolating the modified Langlands correspondence over the stack of L-parameters that is suggested by the work of Helm and of Emerton and Helm.
Association of Walking and Biking to School Policies and Active Commuting to School in Children
Authors: Ganzar Leigh Ann , Burford Katie , Zhang Yuzi , Gressett Anna , Kohl Harold W. , Hoelscher Deanna M.
Published: May 2023

Background: Active commuting to school (ACS) can be an important source of physical activity for children. Schools are an important setting for policy-related ACS promotion. The purpose of this study was to examine the association between school policies and ACS, and to assess whether this relation varied by grade. Methods: This cross-sectional study used data from schools recruited to the Safe Travel Environment Evaluation in Texas School study (n = 94). The percent of trips made by active travel modes was measured through tallies among third to fifth grade classrooms from 5 school districts in Central Texas in 2018–2019. School ACS policies and practices were measured through 8 survey items aggregated into a score. Linear mixed effects models were used to assess the association between policies and ACS. Results: School health policy surveys and ACS data were collected from 69 elementary schools. An average of 14.6% of trips to/from school was made using active travel modes. Schools with higher numbers of policies had significantly higher percentages of students using active travel modes (P = .03), and for each additional policy, the predicted percentage of trips made by active travel modes was 1.46% higher. There was a significant interaction effect between school policy and grade, with stronger correlations among higher grades (P = .002). Conclusions: Results from this study demonstrate a correlation between the school policies designed to support walking and biking and ACS. Results from this study can be used to justify the use of school-based policy interventions to promote ACS.
‘Game changer’ method lets scientists peer into mice
Authors: Öz Esra
Published: May 2023

Imaging technique uses standard cell-specific antibodies
Metamorphism of the Uni Suite as an Indicator of Early Precambrian Collisional Processes in the Vyatka Belt, Northeast of Volgo–Uralia
Authors: Pilitsyna T. A. , Erofeeva K. G. , Samsonov A. V. , Postnikov A. V. , Varlamov D. A.
Published: May 2023

According to the core data from two deep holes, the following mineral assemblages were identified in metapelitic rocks of the Uni Suite in the central part of the Vyatka Belt, sandwiched between Archean blocks in the northeastern part of the Volgo–Uralian Segment of the East European Craton: (1) Pl + Ms + Bt + Qz + Kfs + And ± Chl and (2) Pl + Bt + Qz + Kfs + Grt + Sil ± Ms ± Chl. A set of methods including classical (Grt–Bt, GASP, Ti-in-Bt, and Ms–Bt) and multi-equilibrium thermobarometry (winTWQ 2.34), as well as the isopleth intersection method on pseudosection diagrams (GeoPS 3.2.2.128), was applied to estimate the P–T conditions for these assemblages. The calculated P–T parameters of metamorphism are 520–650°C (or up to 690°C, according to the isopleth method) and 2–5.4 kbar. Metamorphism of paragneiss containing assemblage (2) was accompanied by anatexis in the water-saturated system. Metamorphism of rocks of the Uni Suite belongs to the relatively shallow type of the andalusite–sillimanite facies series and amphibolite facies and relates to deformations during orogenes.
The Series of Catastrophic Earthquakes of February 6, 2023, in Turkey and Variations in the Geophysical Fields
Authors: Adushkin V. V. , Spivak A. A. , Rybnov Yu. S. , Tikhonova A. V.
Published: May 2023

An analysis of variations in the microbaric background and the terrestrial magnetic and electric fields in the surface layer of the atmosphere accompanying strong earthquakes that occurred on February 6, 2023, in Turkey, is presented. The analysis involved the results of instrumental observations performed at the Moscow Center for Geophysical Monitoring and the Mikhnevo Observatory (Institute of Geospheric Dynamics, Russian Academy of Sciences), as well as the data obtained by a number of magnetic observatories of the INTERMAGNET network. It is shown that, in addition to the seismic effect, these earthquakes were accompanied by variations in the magnetic and electric fields, as well as by the generation of infrasonic waves recorded at a considerable distance from the sources.
Sulfur Isotope Sources of Sulfides of Neoarchean Iron Deposits of the Kostomuksha Greenstone Belt of Karelia, Russia
Authors: Vysotskiy S. V. , Khanchuk A. I. , Velivetskaya T. A. , Ignatiev A. V. , Aseeva A. V. , Nesterova N. S.
Published: May 2023

The first data on the multi-isotopic S composition of sulfides of the Neoarchean banded iron formations (BIFs) of the Kostomuksha greenstone belt of Karelia (Karelian Craton of the Fennoscandian Shield) formed 2760–2740 Ma ago are presented in this paper. Pyrite associated with fine-grained magnetite has negative δ34S (to –7.8‰) and positive Δ33S (to +0.7‰) values. Pyrrhotite from stringer-disseminated ores associated with schists exhibits negative values of both δ34S (to –6‰) and Δ33S (to –0.46‰). Our data provide grounds to suggest that the BIF sulfides sourced photolitic elementary sulfur from the atmosphere, sulfate from seawater, and sulfur from magma. The S isotope ratios of sulfides reflect the interaction between abiotic (atmospheric, hydrothermal) and biotic (microbial dissimilation reduction) processes during the formation of iron deposits of the Kostomuksha greenstone belt of Karelia.
Endovascular management of a sphenopalatine artery pseudoaneurysm: a rare cause of delayed intractable epistaxis following endoscopic transsphenoidal pituitary surgery
Authors: Ramachandran Sreehari Nirmala , Retnathankom Ayyadurai , Rege Ishant , Reddy Krishna Tej
Published: May 2023

Vascular injuries during pituitary surgery are feared as they can lead to serious disability and can be life threatening. We are describing a case of severe intractable epistaxis following endoscopic transnasal transsphenoidal surgery for pituitary tumour due to a sphenopalatine artery pseudoaneurysm which was successfully managed using endovascular embolisation techniques. Very few cases of sphenopalatine artery pseudoaneurysm following endoscopic nasal surgery have been described. A middle aged male patient with a pituitary macroadenoma underwent endoscopic transsphenoidal pituitary surgery and returned to us after 3 days of discharge with severe epistaxis. Digital subtraction angiography showed contrast leakage and left sphenopalatine artery pseudoaneurysm. Glue embolisation of the distal sphenopalatine branches and pseudoaneurysm was done. Good occlusion of pseudoaneurysm was seen. Such a diagnosis for epistaxis following endoscopic transnasal surgery should be borne in mind, so prompt treatment can be planned to avoid life threatening complications.
Preoperative and postoperative optimisation of patients undergoing thyroid surgery: a multicentre quality improvement project at Barts Health NHS Trust
Authors: Lechner Matt , Moghul Gulwish , Chandrasekharan Deepak , Ashraf Salman , Emanuel Oscar , Magos Tiarnan , Liu Zi Wei , Crutchlow Margaret , Kinghorn Stephen , McDonnell Gayle , McArdle Harry , Salem Amr , Papesch Mike , Majumdar Kalpita , Lakhdar Abdulfattah , Ali Safina , Ahmed Jahangir , Alusi Ghassan , Stimpson Paul , Waterhouse Mona , Ghufoor Khalid
Published: May 2023

Hypocalcaemia following thyroid surgery can occur in up to 38% of patients. With over 7100 thyroid surgeries performed in 2018 in the UK, this is a common postoperative complication. Undertreated hypocalcaemia can result in cardiac arrhythmias and death. Preventing adverse events from hypocalcaemia requires preoperative identification and treatment of at-risk patients with vitamin D deficiency, timely recognition of postoperative hypocalcaemia and prompt appropriate treatment with calcium supplementation. This project aimed to design and implement a perioperative protocol for prevention, detection and management of post-thyroidectomy hypocalcaemia. A retrospective audit of thyroid surgeries (n=67; October 2017 to June 2018) was undertaken to establish baseline practice of (1) preoperative vitamin D levels assessment, (2) postoperative calcium checks and incidence of postoperative hypocalcaemia and (3) management of postoperative hypocalcaemia. A multidisciplinary team approach following quality improvement principles was then used to design a perioperative management protocol with all relevant stakeholders involved. After dissemination and implementation, the above measures were reassessed prospectively (n=23; April–July 2019). The percentage of patients having their preoperative vitamin D measured increased from 40.3% to 65.2%. Postoperative day-of-surgery calcium checks increased from 76.1% to 87.0%. Hypocalcaemia was detected in 26.8% of patients before and 30.43% of patients after protocol implementation. The postoperative component of the protocol was followed in 78.3% of patients. Limitations include low number of patients which precluded from analysis of the impact of the protocol on length of stay. Our protocol provides a foundation for preoperative risk stratification and prevention, early detection and subsequent management of hypocalcaemia in thyroidectomy patients. This aligns with enhanced recovery protocols. Moreover, we offer suggestions for others to build on this quality improvement project with the aim to further advance the perioperative care of thyroidectomy patients.
Closure Properties of Subregular Languages Under Operations
Authors: Olejár Viktor , Szabari Alexander
Published: May 2023

A class of languages is closed under a given operation if the resulting language belongs to this class whenever the operands belong to it. We examine the closure properties of various subclasses of regular languages under basic operations of intersection, union, concatenation and power, positive closure and star, reversal, and complementation. We consider the following classes: definite languages and their variants (left ideal, finitely generated left ideal, symmetric definite, generalized definite and combinational), two-sided comets and their variants comets and stars, and the classes of singleton, finite, ordered, star-free, and power-separating languages. We also give an overview about subclasses of convex languages (classes of ideal, free, and closed languages), union-free languages, and group languages. We summarize some inclusion relations between these classes. Subsequently, for all pairs of a class and an operation, we provide an answer whether this class is closed under this operation or not.
Kinematics Bionic Concept Structure Design and Optimization of Vehicle Crash Dummy’s Knee Joint: Bionics and Biomechanics Applied in Collision Safety of Cars
Authors: Hao Tianyi , Liu Zhixin , Liu Hai
Published: May 2023

The structural bionicism of the knee joint of an automobile crash dummy is an important factor affecting the accuracy of the dummy’s knee displacement and knee flexion angle measurements in automobile crash tests. This study focused mainly on the optimization of the bionic structure of the knee joint of an automobile crash dummy to ensure that the dummy has a kinematic response closer to that of the knee joint of a real human. Forty sets of high-speed photographic images of the sphyrion were acquired by performing a trajectory-measurement test at the lower tibial point. Subsequently, the high-flexion motion trajectory of the knee joint was obtained by solving vector equations and by multicurve fitting. This trajectory, combined with the bionic structure design method, optimized the structure of the existing dummy’s knee joint. Thereby, its motion can be altered from a fixed-axis rotation to a non-fixed-axis curve motion close to how the human tibial plateau rotates around the femoral condyle. This increases the degrees of freedom of the dummy’s knee joint from two to three. The knee joint structures before and after the optimization were simulated kinematically using a multibody dynamics method. The results showed that the peak of the motion trajectory deviation of the optimized sphyrion decreased from 3.7% to 1.9%, and the average deviation decreased from 2.0% to 0.2%. This indicates that the structural optimization scheme improved the motion bionics of the crash dummy’s knee joint.
STK24 Promotes Progression of LUAD and Modulates the Immune Microenvironment
Authors: Li Yadong , Liu Yanhu , Wang Kun , Xue Dong , Huang Yiqin , Tan Zhenguo , Chen Yijiang
Published: May 2023

Objective. Recent studies have shown that serine/threonine-protein kinase 24 (STK24) plays an important role in cancer development. However, the significance of STK24 in lung adenocarcinoma (LUAD) remains to be determined. This study is aimed at investigating the significance of STK24 in LUAD. Methods. STK24 was silenced and overexpressed by siRNAs and lentivirus, respectively. Cellular function was assessed by CCK8, colony formation, transwell, apoptosis, and cell cycle. mRNA and protein abundance was checked by qRT-PCR and WB assay, respectively. Luciferase reporter activity was evaluated to examine the regulation of KLF5 on STK24. Various public databases and tools were applied to investigate the immune function and clinical significance of STK24 in LUAD. Results. We found that STK24 was overexpressed in lung adenocarcinoma (LUAD) tissues. High expression of STK24 predicted poor survival of LUAD patients. In vitro, STK24 enhanced the proliferation and colony growth ability of A549 and H1299 cells. STK24 knockdown induced apoptosis and cell cycle arrest at G0/G1 phase. Furthermore, Krüppel-like factor 5 (KLF5) activated STK24 in lung cancer cells and tissues. Enhanced lung cancer cell growth and migration triggered by KLF5 could be reversed by silencing of STK24. Finally, the bioinformatics results showed that STK24 may be involved in the regulation of the immunoregulatory process of LUAD. Conclusion. KLF5 upregulation of STK24 contributes to cell proliferation and migration in LUAD. Moreover, STK24 may participate in the immunomodulatory process of LUAD. Targeting KLF5/STK24 axis may be a potential therapeutic strategy for LUAD.
Supplementary Figures S1-S10 from p38MAPKα Stromal Reprogramming Sensitizes Metastatic Breast Cancer to Immunotherapy
Authors: Faget Douglas V. , Luo Xianmin , Inkman Matthew J. , Ren Qihao , Su Xinming , Ding Kai , Waters Michael R. , Raut Ganesh Kumar , Pandey Gaurav , Dodhiawala Paarth B. , Ramalho-Oliveira Renata , Ye Jiayu , Cole Thomas , Murali Bhavna , Zheleznyak Alexander , Shokeen Monica , Weiss Kurt R. , Monahan Joseph B. , DeSelm Carl J. , Lee Adrian V. , Oesterreich Steffi , Weilbaecher Katherine N. , Zhang Jin , DeNardo David G. , Stewart Sheila A.
Published: May 2023

Contains supplemental figures and legends
Supplementary figures from The Immunopeptidome from a Genomic Perspective: Establishing the Noncanonical Landscape of MHC Class I–Associated Peptides
Authors: Bedran Georges , Gasser Hans-Christof , Weke Kenneth , Wang Tongjie , Bedran Dominika , Laird Alexander , Battail Christophe , Zanzotto Fabio Massimo , Pesquita Catia , Axelson Håkan , Rajan Ajitha , Harrison David J. , Palkowski Aleksander , Pawlik Maciej , Parys Maciej , O'Neill J. Robert , Brennan Paul M. , Symeonides Stefan N. , Goodlett David R. , Litchfield Kevin , Fahraeus Robin , Hupp Ted R. , Kote Sachin , Alfaro Javier A.
Published: May 2023

Supplementary Figures S1 to S7.
Supplementary Table 3 from The Immunopeptidome from a Genomic Perspective: Establishing the Noncanonical Landscape of MHC Class I–Associated Peptides
Authors: Bedran Georges , Gasser Hans-Christof , Weke Kenneth , Wang Tongjie , Bedran Dominika , Laird Alexander , Battail Christophe , Zanzotto Fabio Massimo , Pesquita Catia , Axelson Håkan , Rajan Ajitha , Harrison David J. , Palkowski Aleksander , Pawlik Maciej , Parys Maciej , O'Neill J. Robert , Brennan Paul M. , Symeonides Stefan N. , Goodlett David R. , Litchfield Kevin , Fahraeus Robin , Hupp Ted R. , Kote Sachin , Alfaro Javier A.
Published: May 2023

Annotation of the COD-dipp ncMAPs.
Supplementary Table 2 from The Immunopeptidome from a Genomic Perspective: Establishing the Noncanonical Landscape of MHC Class I–Associated Peptides
Authors: Bedran Georges , Gasser Hans-Christof , Weke Kenneth , Wang Tongjie , Bedran Dominika , Laird Alexander , Battail Christophe , Zanzotto Fabio Massimo , Pesquita Catia , Axelson Håkan , Rajan Ajitha , Harrison David J. , Palkowski Aleksander , Pawlik Maciej , Parys Maciej , O'Neill J. Robert , Brennan Paul M. , Symeonides Stefan N. , Goodlett David R. , Litchfield Kevin , Fahraeus Robin , Hupp Ted R. , Kote Sachin , Alfaro Javier A.
Published: May 2023

Open search peptide-spectrum matches (PSMs) information of serine acetylation, cysteinylation, carbamidomethylation, and cysteine trioxidation.
Data from The Immunopeptidome from a Genomic Perspective: Establishing the Noncanonical Landscape of MHC Class I–Associated Peptides
Authors: Bedran Georges , Gasser Hans-Christof , Weke Kenneth , Wang Tongjie , Bedran Dominika , Laird Alexander , Battail Christophe , Zanzotto Fabio Massimo , Pesquita Catia , Axelson Håkan , Rajan Ajitha , Harrison David J. , Palkowski Aleksander , Pawlik Maciej , Parys Maciej , O'Neill J. Robert , Brennan Paul M. , Symeonides Stefan N. , Goodlett David R. , Litchfield Kevin , Fahraeus Robin , Hupp Ted R. , Kote Sachin , Alfaro Javier A.
Published: May 2023

Abstract
Tumor antigens can emerge through multiple mechanisms, including translation of noncoding genomic regions. This noncanonical category of tumor antigens has recently gained attention; however, our understanding of how they recur within and between cancer types is still in its infancy. Therefore, we developed a proteogenomic pipeline based on deep learning de novo mass spectrometry (MS) to enable the discovery of noncanonical MHC class I–associated peptides (ncMAP) from noncoding regions. Considering that the emergence of tumor antigens can also involve posttranslational modifications (PTM), we included an open search component in our pipeline. Leveraging the wealth of MS-based immunopeptidomics, we analyzed data from 26 MHC class I immunopeptidomic studies across 11 different cancer types. We validated the de novo identified ncMAPs, along with the most abundant PTMs, using spectral matching and controlled their FDR to 1%. The noncanonical presentation appeared to be 5 times enriched for the A03 HLA supertype, with a projected population coverage of 55%. The data reveal an atlas of 8,601 ncMAPs with varying levels of cancer selectivity and suggest 17 cancer-selective ncMAPs as attractive therapeutic targets according to a stringent cutoff. In summary, the combination of the open-source pipeline and the atlas of ncMAPs reported herein could facilitate the identification and screening of ncMAPs as targets for T-cell therapies or vaccine development.
Data from Clonal Spreading of Tumor-Infiltrating T Cells Underlies the Robust Antitumor Immune Responses
Authors: Aoki Hiroyasu , Tsunoda Mikiya , Ogiwara Haru , Shimizu Haruka , Abe Haruka , Ogawa Tatsuro , Abe Takaya , Shichino Shigeyuki , Matsushima Kouji , Ueha Satoshi
Published: May 2023

Abstract
The repertoire of tumor-infiltrating T cells is an emerging method for characterizing effective antitumor T-cell responses. Oligoclonal expansion of the tumor T-cell repertoire has been evaluated; however, their association with antitumor effects is unclear. We demonstrate here that the polyclonal fraction of the tumor-reactive T-cell repertoire, consisting of relatively minor clones, increased in tumor-bearing mice treated with monoclonal anti–programmed death-ligand 1 (PD-L1) or anti-CD4, which correlated with antitumor effects. Meanwhile, the size of the oligoclonal fraction consisting of major clones remained unchanged. Moreover, the polyclonal fraction was enriched in progenitor exhausted T cells, which are essential for a durable antitumor response, and was more dependent on CCR7+ migratory dendritic cells, which are responsible for priming tumor-reactive T cells in the tumor-draining lymph nodes. These results suggest that the expansion of diverse tumor-reactive clones (“clonal spreading”) represents characteristics of antitumor T-cell responses induced by anti-CD4 and anti–PD-L1 treatment.
Supplementary Table S3 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplementary Table 3: Race/ethnicity specific incidence rates, per 100,000, adjusted to U.S. standard 2000 population, with left truncation.
Supplementary Figure S9 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplementary Figure 9: Sex stratified analysis for the association between Any T2D and BCA. Models adjusted for pack-years smoked (0, 1-10, 11-20, 21-30, 31-40, >40), alcohol consumption (<1 drinks/month, ≤1 drinks/day, >1 drinks/day), number of cigarettes smoked per day among smokers (5 cigarettes or less, 6-10 cigarettes, 11-20, 21-30, 31 or more), race/ethnicity (European American, African American, Japanese American, Latin American, Native Hawaiian), and reproductive factors among females. Body mass index (BMI, kg/m3) and smoking status (ever, never, current) were included as strata variables due to their proportional hazard violation. Categorical birthyear was also included as a strata variable to adjust for possible cohort effects. CI: Confidence Interval; HR: Hazard Ratio; P het: P- heterogeneity for likelihood ratio test for difference in association across covariate levels.
Supplementary Figure S8 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplementary Figure 8: Association between Any T2D and BCA stratified by sex and race/ethnicity. Models adjusted for pack-years smoked (0, 1-10, 11-20, 21-30, 31-40, >40), alcohol consumption (<1 drinks/month, ≤1 drinks/day, >1 drinks/day), sex (male, female), number of cigarettes smoked per day among smokers (5 cigarettes or less, 6-10 cigarettes, 11-20, 21-30, 31 or more), race/ethnicity (European American, African American, Japanese American, Latin American, Native Hawaiian), and reproductive factors among females. Body mass index (BMI, kg/m3) and smoking status (ever, never, current) were included as strata variables due to their proportional hazard violation. Categorical birth year was also included as a strata variable to adjust for possible cohort effects. CI: Confidence Interval; HR: Hazard Ratio; P het: P- heterogeneity for likelihood ratio test for difference in association by sex.
Supplementary Figure S7 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplementary Figure 7: Association between T2D and BCA in the multiethnic sample and by race/ethnicity, stratified by T2D assessment method, restricted to MEC participants with Medicare FFS enrollment. Models adjusted for pack-years smoked (0, 1-10, 11-20, 21-30, 31-40, >40), alcohol consumption (<1 drinks/month, ≤1 drinks/day, >1 drinks/day), sex (male, female), number of cigarettes smoked per day among smokers (5 cigarettes or less, 6-10 cigarettes, 11-20, 21-30, 31 or more), race/ethnicity (European American, African American, Japanese American, Latin American, Native Hawaiian), and reproductive factors among females. Body mass index (BMI, kg/m3) and smoking status (ever, never, current) were included as strata variables due to their proportional hazard violation. Categorical birthyear was also included as a strata variable to adjust for possible cohort effects. CI: Confidence Interval; HR: Hazard Ratio; P het: P- heterogeneity for likelihood ratio test for difference in association across race/ethnicity groups.
Supplementary Figure S4 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplemental Figure 4: Response rates to follow-up questionnaire among MEC participants who returned the baseline questionnaire and were alive for each respective survey by race/ethnicity. As an example, on the 3rd questionnaire, 44.3% of living African American participants returned a questionnaire. Bar heights shorten based on number of participants alive during the survey collection period.
QX: Questionnaire.
Supplementary Figure S3 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplemental Figure 3: Distribution of MEC participation across participation opportunities by race/ethnicity, represented as percentage by race/ethnicity. BL: baseline questionnaire; FU QX: follow-up questionnaire; BR: biorepository questionnaire; CMS: Medicare FFS.
Supplementary Figure S1 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Supplementary Figure 1: MEC participant exclusion, showing removal of participants based on data missingness.
FIGURE 1 from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Association between T2D and bladder cancer in the multiethnic sample and by race/ethnicity, stratified by T2D assessment method. Models adjusted for pack-years smoked (0, 1–10, 11–20, 21–30, 31–40, >40), alcohol consumption (<1 drink/month, ≤1 drink/day, >1 drinks/day), sex (male, female), number of cigarettes smoked per day among smokers (5 cigarettes or less, 6–10 cigarettes, 11–20, 21–30, 31, or more), race/ethnicity (European American, African American, Japanese American, Latin American, Native Hawaiian), and reproductive factors among females. BMI (kg/m3) and smoking status (ever, never, current) were included as strata variables due to their proportional hazard violation. Categorical birthyear was also included as a strata variable to adjust for possible cohort effects. CI, confidence interval; HR, hazard ratio; P het: P-heterogeneity for likelihood ratio test for difference in association across race/ethnicity groups.
Data from Interethnic Differences in Bladder Cancer Incidence and the Association between Type 2 Diabetes and Bladder Cancer in the Multiethnic Cohort Study
Authors: Bogumil David , Cortessis Victoria K. , Wilkens Lynne R. , Le Marchand Loïc , Haiman Christopher A. , Maskarinec Gertraud , Setiawan Veronica Wendy
Published: May 2023

Background:
Research on the association between type 2 diabetes (T2D) and bladder cancer (BCA) risk among non-European ancestry populations is sparse to nonexistent, and most prior studies rely on a single baseline assessment of T2D status.
Methods:
We estimated the T2D-BCA association using the Multiethnic Cohort Study of 185,059 men and women in California and Hawaii. Participants were African American, European American, Japanese American, Latin American, and Native Hawaiian, ages 45–75 years at enrollment (1993–1996). T2D was assessed by self-report at baseline, follow-up surveys, and Medicare claims. Cases were identified using Surveillance, Epidemiology and End Results Program cancer registries through 2016. Associations were estimated by race/ethnicity using Cox proportional hazards regression. Adjusted attributable fractions (AAF) and cumulative absolute risk of bladder cancer were estimated across groups.
Results:
Over an average 19.7 years of follow-up 1,890 incident bladder cancer cases were diagnosed. Time-varying T2D was associated with bladder cancer in the multiethnic sample (HR = 1.17; 95% confidence interval, 1.05–1.30); however, the HR did not differ by race/ethnicity (P = 0.85). The AAF was 4.2% in the multiethnic sample and largest among Native Hawaiians (9.8%). Absolute risk of bladder cancer among European Americans without T2D was higher than all other groups with T2D.
Conclusion:
T2D is significantly associated with bladder cancer risk in a multiethnic sample.
Significance:
Those with T2D have higher incidence of bladder cancer, regardless of racial/ethnic group. Reducing T2D prevalence could substantially lower bladder cancer incidence among Native Hawaiians due to T2D being more common in this group. High absolute risk of bladder cancer among European Americans, regardless of T2D status, indicates that elevated bladder cancer risk in this group may be due to factors other than T2D. Future studies must explore reasons for this difference in incidence.
A escravidão como compromisso liberal na construção do ordenamento jurídico brasileiro no pós-independência
Authors: Santos André Cozer dos
Published: May 2023

O presente artigo defende que o ordenamento jurídico brasileiro foi formado a partir do compromisso conservador das camadas dominantes por meio da instrumentalização do discurso liberal. Além disso, pretende-se demonstrar como não houve contradição entre os ideais liberais utilizados por essas classes dominantes e o instituto da escravidão. Para isso, partimos da obra de Eunice Aparecida de Jesus, com uma atualização historiográfica. Percebemos, a partir dessa pesquisa bibliográfica, como a escravidão foi um fator de integração das camadas dominantes e como a instrumentalização do liberalismo e a contraposição aos ideais democráticos permitiu a sustentação política e jurídica da exclusão da maioria da população brasileira dos espaços decisórios e, acima de tudo, permitiu a manutenção da escravidão.