bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
Authors: Müller-Kräuter Helena , Mezzacapo Jolanda , Klüver Michael , Baumgart Sara , Becker Dirk , Fathi Anahita , Pfeiffer Sebastian , Krähling Verena
Published: May 2024

To date, there is no licensed vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV). Therefore, MERS-CoV is one of the diseases targeted by the Coalition for Epidemic Preparedness Innovations (CEPI) vaccine development programs and has been classified as a priority disease by the World Health Organization (WHO). An important measure of vaccine immunogenicity and antibody functionality is the detection of virus-neutralizing antibodies. We have developed and optimized a microneutralization assay (MNA) using authentic MERS-CoV and standardized automatic counting of virus foci. Compared to our standard virus neutralization assay, the MNA showed improved sensitivity when analyzing 30 human sera with good correlation of results (Spearman’s correlation coefficient r = 0.8917, p value < 0.0001). It is important to use standardized materials, such as the WHO international standard (IS) for anti-MERS-CoV immunoglobulin G, to compare the results from clinical trials worldwide. Therefore, in addition to the neutralizing titers (NT50 = 1384, NT80 = 384), we determined the IC50 and IC80 of WHO IS in our MNA to be 0.67 IU/ml and 2.6 IU/ml, respectively. Overall, the established MNA is well suited to reliably quantify vaccine-induced neutralizing antibodies with high sensitivity.
Prognosis of patients with advanced bile tract carcinoma: assessment using the modified-Gustave Roussy Immune Score (mGRIm-s) as a clinico-immunological tool
Authors: Ma Yue , Pan Yuting , Li Yue , Guan Huafang , Dai Guanghai
Published: May 2024

Background

The emergence of immune checkpoint inhibitors (ICIs) has enhanced survival outcomes for certain patients with advanced biliary tract carcinoma (BTC). Pinpointing those who would benefit most from immunotherapy remains elusive. We investigated the predictive value of the modified Gustave Roussy Immune Score (mGRIm-s) in BTC patients treated with ICIs.

Methods

Data from 110 patients at Chinese People's Liberation Army General Hospital, spanning September 2015 to April 2021, were analyzed. The median follow-up duration was 38.7 months as of December 2023. Risk factors included low albumin, high lactate dehydrogenase, and an elevated neutrophil–lymphocyte ratio. Patients were stratified into low (patients with no risk factors) and high (patients with at least one risk factor) mGRIm-s groups based on these factors.

Results

Survival outcomes post-immunotherapy favored the low mGRIm-s group, with significantly improved progression-free survival (PFS) and overall survival (OS) (8.50 months vs. 3.70 months and 21.60 months vs. 8.00 months). COX regression confirmed an elevated risk in the high mGRIm-s group. Subgroup analysis highlighted a notable survival advantage for low mGRIm-s patients receiving first-line immunotherapy.

Conclusions

This study underscores mGRIm-s's potential in predicting immunotherapy response in BTC, paving the way for more targeted approaches.
Planar Drawings with Few Slopes of Halin Graphs and Nested Pseudotrees
Authors: Chaplick Steven , Da Lozzo Giordano , Di Giacomo Emilio , Liotta Giuseppe , Montecchiani Fabrizio
Published: May 2024

The planar slope number$${{\,\textrm{psn}\,}}(G)$$

psn

(
G
)

of a planar graph G is the minimum number of edge slopes in a planar straight-line drawing of G. It is known that $${{\,\textrm{psn}\,}}(G) \in O(c^{\Delta })$$

psn

(
G
)

∈
O

(

c
Δ

)

for every planar graph G of maximum degree $$\Delta $$
Δ
. This upper bound has been improved to $$O(\Delta ^5)$$

O
(

Δ
5

)

if G has treewidth three, and to $$O(\Delta )$$

O
(
Δ
)

if G has treewidth two. In this paper we prove $${{\,\textrm{psn}\,}}(G) \le \max \{4,\Delta \}$$

psn

(
G
)
≤
max
{
4
,
Δ
}

when G is a Halin graph, and thus has treewidth three. Furthermore, we present the first polynomial upper bound on the planar slope number for a family of graphs having treewidth four. Namely we show that $$O(\Delta ^2)$$

O
(

Δ
2

)

slopes suffice for nested pseudotrees.
Climate cyclicity-controlled recurrent bottom-water ventilation events in the aftermath of the Toarcian Oceanic Anoxic Event: the Jenkyns Event
Authors: Schwark Lorenz , Ruebsam Wolfgang
Published: May 2024

Environmental perturbations of the Toarcian Anoxic Event and its associated carbon isotope excursion (CIE) occurred in a cyclic fashion indicating an orbital control mechanism. Sedimentary strata of the E. elegantulum ammonite subzone in the Lorraine Sub-basin, Luxembourg, exhibit eight sedimentary cycles, most of which postdate the CIE, implying that its termination did not coincide with a full recovery from environmental stress. Sea-level and temperature fluctuations of the Toarcian crisis were linked to a cryosphere demise in the Northern Hemisphere, which modulated stadial versus interstadial phases on the orbital 100 ka eccentricity frequency band. Upon stadial phases, enhanced wind strength in combination with lowered sea level disrupted stratified shelf waters and shifted the storm wave base close to the sea floor. Ventilation of bottom waters interrupted accumulation of laminated and organic-rich black shales, but formed organic-lean and non-laminated gray claystones enriched in terrigenous wax lipids and spores provided via aeolian transport from the hinterland due to the enhanced wind strength. Wind systems are assumed to have been driven by high pressure differences between the cryosphere in the North of the Tethyan shelf and the warmer costal lowland. This distinguishes the atmospheric turbulences after the CIE from the postulated intensification of the cyclones that accompanied the rapid warming at the beginning of the CIE. The deep-water ventilation event following the CIE in the Lorraine Sub-basin was accompanied by a parallel evolution in the SW German Basin, indicating a supraregional driving mechanism in paleobathymetric sub-basins susceptible to lowering of storm wave base. In sub-basins with greater water depth on the NW-Tethyan shelf, post-CIE orbitally driven atmospheric turbulences had a minor or no effect on water column stratification and deposition of organic-rich laminated black shales persisted.

Graphical abstract
Topical antimicrobial treatment of mesh for the reduction of surgical site infections after hernia repair: a systematic review and meta-analysis
Authors: Bontekoning Nathan , Huizing Nathalie J. , Timmer Allard S. , Groenen Hannah , de Jonge Stijn W. , Boermeester Marja A.
Published: May 2024

Purpose

Use of mesh is essential in hernia repair. A common complication after hernia repair is surgical site infection (SSI), which poses a risk in spreading to the mesh, possibly causing mesh infection. Topical antimicrobial pretreatment of mesh may potentially reduce SSI risk in hernia repair and has shown promising results in in vitro and in vivo studies. Clinical evidence, however, is more important. This systematic review aims to provide an overview of available clinical evidence for antimicrobial pretreated mesh in hernia repair surgery to reduce SSI.

Methods

We report in accordance with PRISMA guidelines. CENTRAL, EMBASE, CINAHL and PubMed were searched up to October 2023 for studies that investigated the use of antimicrobial pretreated mesh on SSI incidence in adults undergoing hernia repair. The primary outcome was SSI incidence. We also collected data on pathogen involvement, hernia recurrence, and mesh infection. A meta-analysis on SSI risk and GRADE-assessment was performed of eligible studies.

Results

We identified 11 eligible studies (n = 2660 patients); 5 randomized trials and 6 cohort studies. Investigated interventions included pre-coated mesh, antibiotic carriers, mesh soaked or irrigated with antibiotic or antiseptic solution. Meta-analysis showed no significant reduction in SSI for antibiotic pretreated polypropylene mesh (RR 0.76 [95% CI 0.27; 2.09]; I2 50%).

Conclusion

Data on topical mesh pretreatment to reduce SSI risk after hernia repair is limited. Very low certainty evidence from randomized trials in hernia repair surgery shows no significant benefit for antibiotic mesh pretreatment for SSI reduction, but data are imprecise due to optimal information size not being met.
Diclofenac sodium adsorption in aqueous media by activated carbon obtained from einkorn (Triticum monococcum L.) husk
Authors: Kocabıyık Barış , Üner Osman , Geçgel Ünal
Published: May 2024

The uncontrolled release of waste diclofenac with low biodegradability is considered to be a potential threat for the environment and creatures. To find effective solution for this issue, this study reports the adsorption performance of diclofenac sodium salt (DCF) by using activated carbon (EHAC) obtained from einkorn (Triticum monococcum L.) husk in aqueous solution under various circumstances. It was found that DCF adsorption on EHAC was highly solution pH dependent, and DCF adsorption by EHAC decreased with increasing adsorption temperature. Equilibrium data showed that fitted isotherm model with the experiment results of DCF adsorption on EHAC followed the order of Langmuir > Temkin > Freundlich > Dubinin-Radushkevich. Adsorption capacity of EHAC for DCF adsorption in aqueous solution was calculated to be 147.06 mg/g at 25 °C. The adsorption kinetic of DCF adsorption on EHAC was determined to obey the pseudo-second-order kinetic model. By utilizing FTIR and pH data obtained from DCF adsorption on EHAC, DCF adsorption mechanisms with some interactions such as π-π stacking, electrostatic interactions, and hydrogen bonding were suggested at diverse pH values. Additionally, intraparticle diffusion model was applied to kinetic results to further recognize the kinetic mechanism of DCF adsorption on EHAC. Furthermore, thermodynamic parameters for DCF adsorption on EHAC were calculated and evaluated, in which DCF adsorption process by EHAC was determined to be exothermic, spontaneous, and feasible.
Increases in Awareness and Uptake of Dating Apps’ Sexual Health Features Among US Men Who Have Sex with Men, 2018 to 2021
Authors: Hecht Jennifer , Zlotorzynska Maria , Wohlfeiler Dan , Sanchez Travis H.
Published: May 2024

Dating apps are now used by the majority of MSM to meet sexual and romantic partners. While research has demonstrated an association between app use and greater number of sex partners and STIs, dating apps also pose an opportunity for intervention. By advocating for new and improved sexual health features on dating apps, Building Healthy Online Communities (BHOC) aims to increase communication about sexual health on the apps. As a follow-up to our previous paper assessing the uptake of sexual health-related profile options on dating apps through Emory’s annual survey of 10,000 MSM in the US, BHOC and Emory partnered to explore the change in uptake over time, again through their annual survey. Among survey participants in 2021, 85% reported using dating apps to meet a partner in the past year, and among this group, 93% reported awareness of sexual health features, up from 77% in 2018 (p < 0.0001). 71% of app users who were aware of features in 2021 reported using one or more sexual health feature, up from 61% in 2018 (p < 0.0001). BHOC will continue to advocate for increased uptake of these features, especially among subgroups with lower levels of uptake.
A survey on imbalanced learning: latest research, applications and future directions
Authors: Chen Wuxing , Yang Kaixiang , Yu Zhiwen , Shi Yifan , Chen C. L. Philip
Published: May 2024

Imbalanced learning constitutes one of the most formidable challenges within data mining and machine learning. Despite continuous research advancement over the past decades, learning from data with an imbalanced class distribution remains a compelling research area. Imbalanced class distributions commonly constrain the practical utility of machine learning and even deep learning models in tangible applications. Numerous recent studies have made substantial progress in the field of imbalanced learning, deepening our understanding of its nature while concurrently unearthing new challenges. Given the field’s rapid evolution, this paper aims to encapsulate the recent breakthroughs in imbalanced learning by providing an in-depth review of extant strategies to confront this issue. Unlike most surveys that primarily address classification tasks in machine learning, we also delve into techniques addressing regression tasks and facets of deep long-tail learning. Furthermore, we explore real-world applications of imbalanced learning, devising a broad spectrum of research applications from management science to engineering, and lastly, discuss newly-emerging issues and challenges necessitating further exploration in the realm of imbalanced learning.
Aquaculture and Aeromonas hydrophila: a complex interplay of environmental factors and virulence
Authors: Abdella Bahaa , Shokrak Nermeen M. , Abozahra Nourhan A. , Elshamy Yomna M. , Kadira Hossam I. , Mohamed Radi A.
Published: May 2024

Aquaculture faces significant challenges due to bacterial pathogens like Aeromonas hydrophila, which can severely impact production and fish health. Understanding the relationship between environmental factors, host susceptibility, and bacterial virulence is crucial for effectively managing and mitigating the risks associated with A. hydrophila in aquaculture systems. A. hydrophila, found ubiquitously in aquatic environments, possesses various virulence factors that enhance its pathogenicity. These factors are closely linked to environmental conditions, such as temperature and pH, which play pivotal roles in bacterial growth, survival, and pathogenic potential. Fluctuations in temperature and pH significantly influence A. hydrophila’s metabolic activity and growth rate, thereby modulating its virulence and overall pathogenicity. Ammonia, a byproduct of aquatic organism metabolism and organic matter decomposition, can accumulate to toxic levels in aquaculture settings, compromising fish health and immune function. Elevated ammonia concentrations worsen A. hydrophila infections by compromising host immunity and creating favorable conditions for bacterial proliferation. Oxygen concentration, host signals, and diet formulation are significantly impacting the susceptibility of aquatic organisms to infection. These factors are the most crucial in shaping the ecology, physiology, and pathogenicity of A. hydrophila in aquaculture. There is limited information on how these environmental factors modulate the virulence genes of such important fish pathogens. The knowledge of A. hydrophila virulence and its interplay with environmental factors paves the way for developing strategies to prevent and control diseases in aquaculture.
Preventing Sexual Harm in Nightlife Settings: A Scoping Review
Authors: Button Kira , Taylor Nicholas , Guala Tahnee , De Andrade Dominique , Coomber Kerri , Quigg Zara , Miller Peter
Published: May 2024

Sexual harm within nightlife settings is a pervasive global concern; however, little is known about the nature of available interventions. The current study aims to review the literature on the nature and effectiveness of nightlife-related sexual harm interventions. A systematic literature search of six databases was conducted to identify records that were published between 1970 and June 2023 and reported approaches that aimed to reduce or prevent nightlife-related sexual harm. Records were included if they theorized, discussed, or evaluated an intervention, prevention or response strategy; however, individual safety strategies were excluded. Results were categorized according to intervention type. Thirty-five peer-reviewed journal articles and 16 gray literature records were identified. The most common nightlife-related sexual harm intervention strategies covered by the literature included targeted policies, laws, and regulations, bystander interventions, and awareness-raising campaigns. Literature in the area is increasing, with the majority of the articles (77.1%) being published in the previous six years; however, there are very few interventions that have been critically evaluated (22.9%). Promising areas for intervention include targeted alcohol regulations (e.g., lockout policies), venue-level policies, and environmental interventions. However, an increase in rigorous evaluative practices is urgently required to ensure future interventions are based on sound theoretical work and empirical evidence.
Intergenerational Transmission of Social Anxiety: The Role of Parents’ Fear of Negative Child Evaluation and Their Self-Referent and Child-Referent Interpretation Biases
Authors: Dülger Melis , Van Bockstaele Bram , Majdandžić Mirjana , de Vente Wieke
Published: May 2024

Purpose

Parents’ fear of negative evaluation (FNE), fear of negative child evaluation (FNCE; parents’ fear that their child is evaluated negatively by others), and self- and child-referent negative interpretation biases have been proposed to play a role in the intergenerational transmission of social anxiety. In this cross-sectional study, we tested whether parents’ self-reported FNE and self-referent interpretation bias, as well as their FNCE and child-referent interpretation bias, statistically mediated the relationship between parent social anxiety and parent-reported child social anxiety.

Methods

A total of 179 parents of 13–16-year-old adolescents completed questionnaires concerning own social anxiety, their FNE, and FNCE and their child’s social anxiety. Parents’ self- and child-referent interpretation biases were measured using scenario completion and memory recognition tasks.

Results

Parents’ FNCE partially statistically mediated the relationship between parent and child social anxiety. Parents’ FNE and their self- and child-referent interpretation biases did, however, not statistically mediate this relationship.

Conclusions

Parents’ FNCE does, but their FNE and self- and child-referent interpretation biases do not seem to play a role in the intergenerational transmission of social anxiety. Hence, parents’ FNCE might be a promising target for clinical practice while designing therapies and interventions concerning child social anxiety.
Genome-wide association study in Chickpea (Cicer arietinum L.) for yield and nutritional components
Authors: Sari Hatice , Uhdre Renan , Wallace Lyle , Coyne Clarice J. , Bourland Britton , Zhang Zhiwu , Russo M. , Kiszonas Alecia , Warburton Marilyn L.
Published: May 2024

The increase in the global human population and the accompanying challenges in meeting nutritional needs amidst climate change are a worldwide concern. Widespread protein and micronutrient deficiencies contribute to a significant number of individuals experiencing malnutrition, leading to severe health repercussions. This issue can be addressed through genomics-assisted breeding, particularly in enhancing the nutritional profile of vital staple crops like chickpea (Cicer arietinum L.). Chickpea, beyond being a rich source of protein, provides a diverse nutritional spectrum encompassing carbohydrates, fats, and minerals. To explore and improve the genetic basis of nutritional traits in chickpea, a study was conducted using 93 kabuli-type single plant derived lines and five cultivars in 2018, 2022, and 2023. Genotyping by sequencing revealed a total of 165K single nucleotide polymorphisms (SNPs) within this kabuli chickpea mini-core collection. After filtering for a minor allele frequency greater than 5%, 113,512 SNPs were utilized, distributed across eight chromosomes of the chickpea genome. Marker-trait associations were analyzed using genome wide association study, leading to the identification of 27 significantly associated SNPs from across all eight chromosomes linked to three seed nutritional concentrations and 100-seed weight. To unravel the molecular mechanisms governing seed protein, fiber, fat concentrations, and 100-seed weight, 31 candidate genes were determined within a 30 kb window size. This comprehensive approach holds promise for advancing crop breeding strategies to combat malnutrition and improve global food security.
Phenology and the response of photosynthesis to irradiance and temperature gradient in the herbal drug red alga, Chondria armata (Rhodomelaceae, Ceramiales) from Kagoshima, Japan
Authors: Terada Ryuta , Yoshizato Kyosuke , Murakami Kazuma , Nishihara Gregory N.
Published: May 2024

Seasonal changes in the size of the herbal drug red alga Chondria armata (Rhodomelaceae, Ceramiales) were investigated in Kagoshima, Japan, which is near the northern distributional limit in the western Pacific. Additionally, its photosynthetic response to irradiance and temperature was examined using dissolved oxygen sensors and a pulse amplitude modulation (PAM)-chlorophyll fluorometer. This alga was observed in tidepools throughout the year; its height and weight were greatest in December and the lowest in April and May. The net photosynthesis of the photosynthesis–irradiance (P–E) curve determined at 28°C quickly saturated at 113 µmol photons m-2 s-1, with minimal inhibition even at 1000 µmol photons m-2 s-1. The gross photosynthesis of the photosynthesis–temperature (P–T) curved over 8 to 40°C, measured at 500 µmol photons m-2 s-1, peaked at 30.1°C and decreased rapidly below 20°C and above 36°C, respectively. Similarly, the effective quantum yield (ΔF/Fm') after a 3-day culture during 4–40°C at 50 µmol photons m-2 s-1 remained stable between 16°C and 32°C but decreased outside of this range. The combined effect of irradiance (200 [low] and 1000 [high] µmol photons m-2 s-1) and temperature (28, 22, and 16°C) revealed that ΔF/Fm' declined during exposure to high irradiance at all temperature treatments. However, it mostly recovered after a subsequent 12-hour period of dim-light acclimation at 28°C and 22°C. In contrast, those at 16°C could not recover, indicating the occurrence of low-temperature light stress. This alga appears to be well-adapted to the irradiance and temperature environment at the study site. However, the winter temperature appears to approach its threshold level, and the occurrence of strong light during the winter might adversely affect the abundance of this alga near its northern distributional limit.
Non-invasive monitoring of microalgae cultivations using hyperspectral imager
Authors: Pääkkönen Salli , Pölönen Ilkka , Raita-Hakola Anna-Maria , Carneiro Mariana , Cardoso Helena , Mauricio Dinis , Rodrigues Alexandre Miguel Cavaco , Salmi Pauliina
Published: May 2024

High expectations are placed on microalgae as a sustainable source of valuable biomolecules. Robust methods to control microalgae cultivation processes are needed to enhance their efficiency and, thereafter, increase the profitability of microalgae-based products. To meet this need, a non-invasive monitoring method based on a hyperspectral imager was developed for laboratory scale and afterwards tested on industrial scale cultivations. In the laboratory experiments, reference data for microalgal biomass concentration was gathered to construct 1) a vegetation index-based linear regression model and 2) a one-dimensional convolutional neural network model to resolve microalgae biomass concentration from the spectral images. The two modelling approaches were compared. The mean absolute percentage error (MAPE) for the index-based model was 15–24%, with the standard deviation (SD) of 13-18 for the different species. MAPE for the convolutional neural network was 11–26% (SD = 10–22). Both models predicted the biomass well. The convolutional neural network could also classify the monocultures of green algae by species (accuracy of 97–99%). The index-based model was fast to construct and easy to interpret. The index-based monitoring was also tested in an industrial setup demonstrating a promising ability to retrieve microalgae-biomass-based signals in different cultivation systems.
Tritrophic Interactions Mediated by Zoophytophagous Predator-Induced Host Plant Volatiles
Authors: Adams Bashiru , Yusuf Abdullahi Ahmed , Torto Baldwyn , Khamis Fathiya Mbarak
Published: May 2024

The zoophytophagous mirid predator Nesidiocoris tenuis and the ectoparasitoid Stenomesius japonicus are important biological control agents for several agricultural pests including the invasive leafminer, Phthorimaea absoluta, a destructive pest of Solanaceous crops especially tomato in sub-Saharan Africa. However, little is known about how feeding by N. tenuis can influence the tritrophic interactions in the tomato plant. Here, we tested the hypothesis that N. tenuis phytophagy would influence the tritrophic olfactory interactions between the host plant tomato and pest, predator, and parasitoid. In olfactometer assays, P. absoluta females and N. tenuis adults were both attracted to constitutive volatiles released by the tomato plant. Whereas females of P. absoluta avoided volatiles released by N. tenuis-infested plants, S. japonicus females and N. tenuis adults were attracted to the induced volatiles. In coupled gas chromatography-electroantennographic detection (GC-EAD) recordings of intact and N. tenuis-infested plant volatiles, antennae of P. absoluta and S. japonicus females both detected eight components, whereas N. tenuis adults detected seven components which were identified by GC-mass spectrometry (GC-MS) as terpenes and green leaf volatiles (GLVs). Dose-response olfactometer bioassays revealed that the responses of P. absoluta, N. tenuis, and S. japonicus varied with the composition and concentration of blends and individual compounds tested from N tenuis-induced volatiles. Females of P. absoluta showed no preference for an eight-component blend formulated from the individual repellents including hexanal, (Z)-3-hexenyl butanoate, and δ-elemene identified in the volatiles. On the other hand, S. japonicus females were attracted to an eight-component blend including the attractants (E)-2-hexenal, (Z)-3-hexenol, methyl salicylate, β-phellandrene, and (E)-caryophyllene. Likewise, N. tenuis adults were attracted to a seven-component blend including the attractants β-phellandrene, δ-elemene, and (E)-caryophyllene identified in the volatiles. Our findings suggest that there is potential for the use of terpenes and GLVs to manage the insects in the tritrophic interaction.
Cue-Reactive Phenomenology Mediates the Relationship Between Positive Schizotypy and Cue-Reactive Urge to Gamble in Poker-Machine Gamblers
Authors: McTigue Benjamin A. , Talk Andrew C. , Rice Kylie , Rock Adam J.
Published: May 2024

Although ubiquitous in numerous nightlife cultures, poker-machines present a high risk for problematic use and addiction. Previous research has demonstrated that gambling cues (e.g., flashing lights) can activate gambling urges in poker-machine gamblers. However, the processes that contribute to the maintenance of cue-reactive urges to gamble remain unclear. Consequently, the present study explored whether positive schizotypy predicted gambling urge, and whether cue-reactive altered state of awareness, cue-reactive altered time sense, and cue-reactive absorption mediated this relationship. Seventy adults aged between 19 and 68 (M = 48.86, SD = 12.82) participated in an online cue-reactivity experiment. Participants first completed the Problem Gambling Severity Index and the Unusual Experiences subscale of the Short Oxford-Liverpool Inventory of Feelings and Experiences. Subsequently, at three time points (i.e., baseline, directly after a neutral cue, and directly after a gambling cue) participants completed the Altered State of Awareness, Altered Time Sense, and Absorption subscales of the Phenomenology of Consciousness Inventory and a visual analogue scale measuring cue-reactive urge to gamble. It was found that positive schizotypy was significantly positively correlated with cue-reactive urge to gamble. Additionally, cue-reactive altered state of awareness, cue-reactive altered time sense, and cue-reactive absorption mediated this relationship. The theoretical, clinical and practical implications are discussed.
MMC Array to Study X-Ray Transitions in Muonic Atoms
Authors: Unger Daniel , Abeln Andreas , Cocolios Thomas Elias , Eizenberg Ofir , Enss Christian , Fleischmann Andreas , Gastaldo Loredana , Godinho César , Heines Michael , Hengstler Daniel , Indelicato Paul , Jadhav Ashish , Kreuzberger Daniel , Kirch Klaus , Knecht Andreas , Machado Jorge , Ohayon Ben , Paul Nancy , Pohl Randolf , von Schoeler Katharina , Vogiatzi Stergiani Marina , Wauters Frederik
Published: May 2024

The QUARTET collaboration aims to significantly improve the precision of the absolute nuclear charge radii of light nuclei from Li to Ne by using an array of metallic magnetic calorimeters to perform high-precision X-ray spectroscopy of low-lying states in muonic atoms. A proof-of-principle measurement with lithium, beryllium and boron is planned for fall 2023 at the Paul Scherrer Institute. We discuss the performance achieved with the maXs-30 detector module to be used. To place the detector close to the target chamber where the muon beam will impact the material under study, we have developed a new dilution refrigerator sidearm. We further discuss the expected efficiency given the transparency of the X-ray windows and the quantum efficiency of the detector. The expected muonic X-ray rate combined with the high resolving power and detection efficiency of the detector suggest that QUARTET will be able to study the de-excitation of light muonic atoms at an unprecedented level, increasing the relative energy resolution by up to a factor of 20 compared to conventional detector techniques.
In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth
Authors: Hardwick Laura J. A. , Davies Benjamin P. , Pensa Sara , Burge-Rogers Maedee , Davies Claire , Baptista André Figueiredo , Knott Robert , S. McCrone Ian , Po Eleonora , Strugnell Benjamin W. , Waine Katie , Wood Paul , Khaled Walid T. , Summers Huw D. , Rees Paul , Wills John W. , Hughes Katherine
Published: May 2024

Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.
Innovative control strategy for flood mitigation through a combination of barrage management on impounded rivers and polders
Authors: Dickel Sarah , Theobald Stephan
Published: May 2024

The partially catastrophic effects of large floods, such as the events of 2002 and 2013 in Germany and Austria for example, clearly show the vulnerability of settlement structures along large rivers. In the case of impounded rivers with run-of-river hydropower plants, the main question is whether a reduction of floods can be achieved with flood-adapted, intelligent control of barrages - to date, the operational objective has been the safe discharge of floodwater; targeted reduction is currently an unoccupied field due to its complexity. Specifically, the interaction with the operation of a polder for flood retention is of special interest, as polders are a much-discussed and effective element of flood protection, especially at higher discharges, that directly influences the hydrograph shape. Extensive analyses and sensitivity investigations of a multitude of parameters and measured values, using a complex model system, are needed in order to investigate the reduction potential of the named technical flood protection measures and the development of control strategies relevant to practice. It is essential to base the developed controlling strategies on operationally available data, as this is the only way to make the determined reductions relevant for practical operation.
The 2015 Refugee Crisis and Institutional Trust in European Countries
Authors: Economou Athina , Kollias Christos
Published: May 2024

Exogenous events have the potential to affect citizens’ risk-perceptions, (in)security sentiments, electoral choices, policy preferences and institutional trust levels. It has been shown that migrant and refugee flows also affect citizens’ trust and policy preferences. The 2015 refugee crisis as it has come to be known, shook Europe’s cohesion and tested the existing immigration and asylum policies as well as acted as a further stimulus to populist/right wing Eurosceptic parties with an agenda of anti-immigrant restrictive policies. Expectedly, it has attracted considerable attention in the relevant literature. Building on existing studies that examine various aspects associated with the 2015 refugee crisis, the present paper examines its effect on institutional trust levels in Europe. Specifically, it focuses on trust towards the national government, the EU and the two state security services the police and the armed forces. To this effect, the study draws data from successive Eurobarometer surveys before and after the crisis. Employing segmented-type regression models as well as Oaxaca decomposition models it probes into the 2015 refugee crisis’ impact on the levels of institutional trust using over 400,000 responses to the relevant Eurobarometer questions. The results reported herein suggest the presence of “a rally around the flag effect”. Nonetheless, macroeconomic factors such as GDP growth and unemployment as well as differences in behavioural responses also explain a large part of the observed gap in trust before and after the 2015 refugee crisis.
Sustainable astronomy: A comparative life cycle assessment of off-grid hybrid energy systems to supply large telescopes
Authors: Viole Isabelle , Shen Li , Camargo Luis Ramirez , Zeyringer Marianne , Sartori Sabrina
Published: May 2024

Purpose

Supplying off-grid facilities such as astronomical observatories with renewable energy–based systems (RES) instead of diesel generators can considerably reduce their environmental impact. However, RES require oversized capacities to counter intermittency and comply with reliability requirements, hence shifting the environmental impact from operation to construction phase. We assess whether 100% RES scenarios are favorable from an environmental point of view and discuss the trade-offs in systems with backup fossil generators versus 100% renewable ones.

Methods

In this comparative life cycle assessment (LCA), we study various RES supply systems to power a new telescope in the Atacama Desert, Chile. We compare six setups, including 100% RES scenarios, namely, photovoltaics (PV) with batteries and hydrogen energy storage; high-renewable scenarios, with fossil fuel power generation next to RES and storage; and a system combining PV with diesel generation. We base system sizing on a techno-economical optimization for the start of operation in 2030. Foreground data stem from life cycle inventories of RES components since 2015 and 2030 electricity mix assumptions of production countries. We assess environmental impact in the categories climate change, mineral resource depletion, and water use.

Results and discussion

We find that 100% RES and high-renewable scenarios result in emissions of 0.077–0.115 kg CO2e/kWh supplied, compared to 0.917 kg CO2e/kWh in the reference case with solely diesel generation. One hundred percent RES scenarios have a lower CO2e impact than high-renewable scenarios. However, the latter lower the mineral resource depletion and water use by about 27% compared to 100% RES scenarios. Applying hybrid energy storage systems increases the water use impact while reducing the mineral resource depletion.

Conclusions

None of the six energy systems we compared was clearly the best in all environmental impacts considered. Trade-offs must be taken when choosing an energy system to supply the prospective off-grid telescope in Chile. We find high-renewable systems with some fossil generation as the better option regarding power reliability, mineral resource depletion, and water use, while inducing slightly higher greenhouse gas emissions than the 100% RES scenarios. As remote research facilities and off-grid settlements today are mainly supplied by fossil fuels, we expect to motivate more multifaceted decisions for implementing larger shares of RES for these areas. To advance the LCA community in the field of energy systems, we should strive to incorporate temporal and regional realities into our life cycle inventories. To ease the path for upcoming studies, we publish this work’s inventories as detailed activity level datasets.
How Social Connectedness Helps Patients Stay Home After Hospital at Home Enrollment: A Mixed Methods Study
Authors: Ledford Christy J. W. , Cafferty Lauren A. , Lee Eunice , Hayes Hailie C. , Ede Destine C. , Hodges Brandon P. , Whitebloom Grant C. , Walsh David W. , Wilkins Thad
Published: May 2024

Background

While enrolled in Hospital at Home (HaH) programs, patients rely on their social network to provide supportive behaviors that are routinely provided by hospital staff in the inpatient setting.

Objective

This study investigated how social connectedness is associated with patient outcomes in a HaH program.

Design

The explanatory iterative sequential mixed methods design included an electronic health record review to collect quantitative measures to describe the severity of patient illness and healthcare utilization and then qualitative interviews to explain quantitative findings.

Participants

The quantitative phase included 100 patients (18 years or older) admitted to the hospital who were subsequently enrolled in the HaH program. In the qualitative phase, 33 of the 100 patients participated in semi-structured interviews.

Analysis

Qualitative data was analyzed using the Sort & Sift, Think & Shift method. Integrated analysis included merged data displays of healthcare utilization data and patient descriptions of their care and genogram-type illustrations to enable variable-oriented analysis of structural support. We then examined patient narratives by two variables: life course and care elevation, to understand differences in the trajectories of six subsets of patients as identified by the quantitative data.

Key Results

Three factors prompted patients to enroll in HaH: low attention from hospital staff during hospital stay; loneliness and isolation during hospital stay; and family encouragement to enroll. After discharge, social support within the home structure facilitated recovery during HaH. Conversely, HaH patients with limited support within the home were more likely to be readmitted.

Conclusions

Structural social connectedness facilitates patient recovery in HaH. Before enrolling patients in HaH, clinicians should take an in-depth social history, including questions about social/familial roles, household responsibilities, and technology acceptance. Clinicians should engage formal and informal caregivers in these conversations early and communicate a clear picture of what caregivers should do to support the patient through recovery.
Factors Influencing Referral for Bariatric Surgery by Primary Care Physicians in Northern Israel
Authors: Zoabi Elham , Elran-Barak Roni , Sakran Nasser , Sandler Noga Kaftori , Hatoum Ossama Abu , Kaplan Uri
Published: May 2024

Purpose

Obesity is a chronic metabolic disease with global distribution among adults and children which affects daily functioning and ultimately quality of life. Primary care physicians (PCPs) provide an important role for the treatment of severe obesity. Better understanding of obesity and its treatment options may increase patients’ referral rates to the various treatment modalities, including metabolic/bariatric surgery (MBS).

Materials and Methods

A quantitative cross-sectional study used a self-reported questionnaire among PCPs of Clalit Health Services (CHS) in Northern Israel. The quantitative questionnaire examined the PCP’s knowledge, opinions, attitude, and approaches to managing severe obesity.

Results

A total of 246 PCPs from Northern Israel filled the questionnaire (42.9%), the majority were Muslim Arabs (54.5%), who gained their medical degree outside of Israel (73.8%) and practicing for over 10 years (58.8%). 64.3% of PCPs had a high workload (over 100 appointments per week), 77.1% did not know the definition of severe obesity, and 69.17% did not attend educational meetings regarding obesity during the previous year. The referral rate for MBS was 50.4% ± 23.3. Two prognostic factors that had a statistically significant effect on the referral rate for bariatric surgery were the total appointments per week, and the number of practice years. Both had a negative association.

Conclusion

The knowledge and referral rates for bariatric surgery are higher among PCPs with lower workload and relatively fewer practice years. Workshops and annual training courses may fortify knowledge and awareness for the treatment of obesity, which in turn could increase the referral rate for MBS.
Anpassung von Modellbakterien an das Jahrhundertherbizid Glyphosat
Authors: Schwedt Inge , Commichau Fabian M.
Published: May 2024

Investigations on the adaptation mechanisms of well-studied model bacteria to the once in-a-century herbicide glyphosate can reveal novel functional relationships between seemingly well-studied cellular processes.
Die Rolle von mikro-RNAs während der B-Zell-Entwicklung
Authors: Wittmann Jürgen
Published: May 2024

B cell development in vertebrates is a complex process, involving numerous differentiation stages at many anatomical locations. Long-known regulators of B cell gene expression include transcription factor networks, cytokines, and cell surface receptors. In recent decades, small non-coding microRNAs (miRNAs) have been shown to exert an additional layer of control over gene expression and have been demonstrated to act as critical gatekeepers at various checkpoints during the tightly regulated B cell development.
Forensische RNA-Analyse–Möglichkeiten und Perspektiven
Authors: Courts Cornelius
Published: May 2024

RNA analysis is used to contextualize biological traces in forensic investigations by assigning biological trace material and components of mixed stains containing different body fluids to particular tissues. While RNA based forensic body fluid and organ tissue identification is already a mature and robust method that is routinely used in forensic casework, there is still active ongoing research exploring the potential of forensic RNA analysis to investigate even more contextual aspects of forensic relevance.
Neuer Standard-Abbautests für synthetische Phosphonate
Authors: Riedel Ramona , Braun Burga , Böllmann Jörg , Martienssen Marion
Published: May 2024

The evaluation of the ready biodegradation of organic substances is usually carried out using the standardised OECD test methods 301 (A–F). However, this can become a great challenge for substances such as aminophosphonates which are chemically imbalanced and do not automatically promote microbial growth in standardized tests. Here, we report on the development of a standardized batch test that is suitable for phosphonates and overcomes important obstacles of the test methods above-mentioned.
Mechanism study of lncRNA RMRP regulating esophageal squamous cell carcinoma through miR-580-3p/ATP13A3 axis
Authors: Tan ZiRui , Luan ShengJie , Wang XiaoPeng , Jiao WenPeng , Jiang Pu
Published: May 2024

Objective

It is well-known that lncRNAs regulate energy metabolism in tumors. This study focused on the action of RMRP on esophageal squamous cell carcinoma (ESCC) cell proliferation, apoptosis, and glycolysis.

Methods

In the resected ESCC tissues and adjacent tissues from patients, RMRP/miR-580-3p/ATP13A3 expressions were evaluated. ESCC cell proliferation rates and apoptotic rates were measured by CCK-8 and flow cytometry, respectively. Apoptosis related markers were examined by Western blot. Moreover, glucose uptake, lactic acid, and ATP were measured by commercial kits, whereas HK2 and PKM2 were evaluated by Western blot to study ESCC cell glycolysis. Finally, the editing program of RMRP/miR-580-3p/ATP13A3 was translated by luciferase reporter assay and RIP analysis.

Results

RMRP and ATP13A3 were induced, while miR-580-3p was reduced in their expression in ESCC tissues. Silencing RMRP reduced proliferation, glycolysis, and anti-apoptosis ability of ESCC cells. RMRP sequestered miR-580-3p to target ATP13A3. Silenced ATP13A3 or overexpressed miR-580-3p rescued overexpressed RMRP-mediated promotion of proliferation, glycolysis, and anti-apoptosis of ESCC cells.

Conclusion

RMRP accelerates ESCC progression through the miR-580-3p/ATP13A3 axis, renewing a reference for lncRNA-based therapies for tumors.
Fe-Incorporated Metal-Organic Cobalt Hydroxide Toward Efficient Oxygen Evolution Reaction
Authors: Jiang Tao , Yao Yuechao , Wu Feiyan , Aziz Iram , Zhang Wenjing
Published: May 2024

Metal-organic cobalt hydroxide emerges as a cost-effective electrocatalyst for the oxygen evolution reaction (OER) in energy conversion. However, the limited active sites and poor conductivity hinder their large-scale application. This study employed salicylate as a bridging ligand to synthesize iron-incorporated metal-organic cobalt hydroxide. The influence of Fe intercalation on Co(OH)(Hsal) (where Hsal denotes o-HOC6H4COO−) was investigated using X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Fe0.2Co0.8(OH)(Hsal) demonstrates remarkable electrocatalytic activity, displaying an OER overpotential of 298 mV at 10 mA cm−2 and a Tafel slope of 57.46 mV dec−1. This enhancement can be attributed to improved charge transfer kinetics and increased active sites. This work highlights the crucial role of Fe in improving the efficiency of Co-based oxygen-evolving catalysts (OECs) and its potential for boosting efficient hydrogen generation in alkaline environments.

Graphical Abstract
Coordination in social learning: expanding the narrative on the evolution of social norms
Authors: Müller Basil
Published: May 2024

A shared narrative in the literature on the evolution of cooperation maintains that social learning evolves early to allow for the transmission of cumulative culture. Social norms, whilst present at the outset, only rise to prominence later on, mainly to stabilise cooperation against the threat of defection. In contrast, I argue that once we consider insights from social epistemology, an expansion of this narrative presents itself: An interesting kind of social norm — an epistemic coordination norm — was operative in early and important instances of specialised social learning. I show how there’s a need for such norms in two key social learning strategies and explain how this need is constituted. In assessor-teaching (e.g. Castro et al., 2019b, 2021), epistemic coordination norms allow agents to coordinate around the content of social learning, i.e., what is to be known and how this is to be done. These norms also allow agents to coordinate around the form of cultural learning in what’s sometimes called strategic social learning (Laland, 2004; Hoppitt & Laland, 2013; Heyes, 2018, Chap. 5) and elsewhere. Broadly speaking, this concerns how cultural learning is organised within the social group. The upshot is that the evolution of social learning and social norms are intertwined in important and underappreciated ways from early on. The above matters as it informs our views about the evolution of social norms more generally. Truly social norms emerged to coordinate a plurality of complex behaviours and interactions, amongst them specialised social learning. I substantiate this view by contrasting it with Jonathan Birch’s views on the evolution of norms. What results is a general but cohesive narrative on the early evolution of social norms.