bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Performance of High-Performance Mortar (HPM) Prepared Using Sodium-Silicate-Modified Graphite Tailing Sand
Authors: Jiang Ruixin , Wang Zhengjun , Du Yingxin , Wen Yajing
Published: October 2024

In order to rationalize the consumption of graphite tailing sand and reduce its pollution of the environment—with sodium silicate being a commonly used activator for improving the strength of concrete composites—in this study, the joint effects of sodium silicate (SS) and graphite tail sand (GT) on the strength and frost resistance of graphite tail sand high-performance mortar (GT-HPM) were investigated. Experiments were conducted to evaluate the bulk density, water absorption, compressive strength, speed of sound, and working performance status of GT-HPM before and after freezing and thawing at different SS dosages and different GT substitution rates. The microstructural properties of GT-HPM were also analyzed by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM/EDS). The results showed that 4% SS doping improved the performance of GT-HPM more obviously. Moreover, with an increase in the GT substitution rate, the mechanical properties and frost resistance of GT-HPM increased firstly and then decreased, and the best performance of GT-HPM was obtained when the GT substitution rate was 20%. At 6% SS doping, the performance of GT-HPM gradually decreased with the increase in the graphite tailing sand substitution rate. FT-IR testing showed that there was no significant change with the type of hydration products used, and the Si–O–T absorption peak and average bond length of GT-4 were the largest. SS and GT promoted the generation of hydration products. Microstructural analysis showed that 4% SS promoted the hydration reaction; in addition, an appropriate amount of GT improved the pore structure of HPM, increased the strength and frost resistance, and provided fundamental insights for the subsequent comprehensive utilization of graphite tailing sand.
Damage Inspection and Seismic Assessment of Lingzhao Xuan in the Palace Museum: A Case Study
Authors: Huang Xuanming , Wang Ruwei , Zhang Xiaogu , Huang Guanghua , Teng Dongyu , Zhang Xinjiang
Published: October 2024

Lingzhao Xuan, located in the Palace Museum, has significant historical, artistic and scientific values. To investigate the safety status and seismic performance of the Lingzhao Xuan structure, the material composition and performance of the main load-bearing members of the Lingzhao Xuan were examined, and a finite element seismic simulation was performed on the model. The results show that under different seismic intensities, the deformation of the central pavilion is more evident than that of the four metal pavilions in the corner. Under the 8-degree frequent seismic intensities, the tensile stress in the local area of the masonry has exceeded the design tensile strength value of the masonry, posing a risk of structural cracking. Under the 8-degree rare seismic intensity, when the seismic waves are along the direction of the east–west engineering axis, the stress at the column foot of the central pavilion is greater than the tensile strength of the cast iron. When the seismic waves are along the direction of the south–north engineering axis, the stress of the tie beam in the north–south direction of the central pavilion exceeds the tensile strength of the cast iron. The structure has a greater risk of collapse due to excessive deformation.
Global and Local Shear Behavior of the Frozen Soil–Concrete Interface: Effects of Temperature, Water Content, Normal Stress, and Shear Rate
Authors: Zhang Kun , Yan Jianglin , Mu Yanhu , Zhu Xiaoming , Zhang Lianhai
Published: October 2024

The interface between soil and concrete in cold climates has a significant effect on the structural integrity of embedded structures, including piles, liners, and others. In this study, a novel temperature control system was employed to conduct direct shear tests on this interface. The test conditions included normal stress (25 to 100 kPa), temperature (ranging from 20 to −6 °C), water content (from 10 to 19%), and shear rates (0.1 to 1.2 mm/min). Simultaneously, the deformation process of the interface was continuously photographed using a modified visual shear box, and the non-uniform deformation mechanism of the interface was analyzed by combining digital image correlation (DIC) technology with the photographic data. The findings revealed that the shear stress–shear displacement curves did not exhibit a discernible peak strength at elevated temperatures, indicating deformation behavior characterized by strain hardening. In the frozen state, however, the deformation softened, and the interfacial ice bonding strength exhibited a positive correlation with decreasing temperature. When the initial water content was 16% and the normal stress was 100 kPa, the peak shear strength increased significantly from 99.9 kPa to 182.9 kPa as the test temperature dropped from 20 °C to −6 °C. Both shear rate and temperature were found to have a marked effect on the peak shear strength, with interface cohesion being the principal factor contributing to this phenomenon. At a shear rate of 0.1 mm/min, the curve showed hardening characteristics, but at other shear rates, the curves exhibited strain-softening behavior, with the softening becoming more pronounced as shear rates increased and temperatures decreased. Due to the refreezing of interfacial ice, the residual shear strength increased in proportion to the reduction in shear rate. On a mesoscopic level, it was evident that the displacement of soil particles near the interface exhibited more pronounced changes. At lower shear rates, the phenomenon of interfacial refreezing became apparent, as evidenced by the periodic changes in interfacial granular displacement at the interface.
Differentiated Impacts of Indoor and Outdoor Fitness Environments on Residents’ Activity Intensity: A Perspective on Homo Urbanicus
Authors: Wei Wei , Xu Wenyi , Hong Mengyao
Published: October 2024

This study develops a framework for the impact mechanism based on the “homo urbanicus” theoretical perspective, linking indoor and outdoor fitness environmental elements to residents’ subjective perceptions and their activity intensity. An empirical investigation is conducted using structural equation modeling (SEM) with data from 286 valid questionnaires (N = 421). The results demonstrate that both indoor and outdoor fitness environmental elements—specifically spatial, facility, and environmental factors—significantly positively influence residents’ fitness activity intensity, with this relationship mediated by perceptions of safety, convenience, comfort, and aesthetics. Furthermore, the study uncovers variations in the types and degrees of influence across indoor and outdoor settings. Based on these findings, the author proposes several planning and design strategies: “In indoor environments, it is essential to ensure that fitness spaces feature bright lighting and thoughtful design to enhance aesthetic appeal; additionally, optimizing spatial layouts and integrating information services can foster greater social interaction. For outdoor environments, prioritizing locations near aesthetically pleasing architecture, adjacent to blue landscapes, and within open areas, as well as sites that are close to community amenities and abundant surrounding facilities, will enhance residents’ fitness activity intensity”.
Back and Forth from Urban Renewal: The Spatial Parameters of Affordable Housing in Two Cities
Authors: Levine Daphna , Yavo Ayalon Sharon
Published: October 2024

This study examines the impact of spatial parameters on urban well-being by comparing the urban design and housing policies of Roosevelt Island in New York City and Bat Yam in the Tel Aviv metropolitan area, Israel. Despite their distinct political and cultural contexts, these cities exhibit similar urban design approaches that integrate physical well-being parameters—such as density, building height, open spaces, and walkability—alongside social well-being parameters including age distribution, income levels, and ethnic diversity. The research traces the evolution of affordable housing through various historical phases, including Urban Renewal, Community Development, and Neoliberal Urbanism, and explores how different residential ownership structures have influenced demographic shifts, gentrification, and neighborhood transformation over the past decades. Using a mixed-methods approach that combines historical analysis with spatial and demographic data, this study highlights the contrasting impacts of Roosevelt Island’s affordable housing program and Bat Yam’s state-led urban renewal policies. The results illustrate how distinct housing models affect community resilience, social stability, and overall urban well-being. By uncovering parallel narratives and extracting valuable insights, this analysis offers lessons for other cities navigating similar market-led pressures and policy shifts. The findings underscore the importance of understanding the interplay between spatial design, housing policies, and ownership structures in shaping the long-term sustainability and quality of life in urban neighborhoods.
Magnetic Resonance Elastography for the Detection and Classification of Prostate Cancer
Authors: Kim Seung Ho , Kim Joo Yeon , Hwang Moon Jung
Published: October 2024

We investigated the feasibility of magnetic resonance elastography (MRE) using a pelvic acoustic driver for the detection and classification of prostate cancer (PCa). A total of 75 consecutive patients (mean age, 70; range, 56–86) suspected of having PCa and who underwent multi-parametric MRI including MRE and subsequent surgical resection were included. The analyzed regions consisted of cancer (n = 69), benign prostatic hyperplasia (BPH) (n = 70), and normal parenchyma (n = 70). A histopathologic topographic map served as the reference standard for each region. One radiologist and one pathologist performed radiologic–pathologic correlation, and the radiologist measured stiffness values in each region of interest on elastograms automatically generated by dedicated software. Paired t-tests were used to compare stiffness values between two regions. ROC curve analysis was also used to extract a cutoff value between two regions. The stiffness value of PCa (unit, kilopascal (kPa); 4.9 ± 1.1) was significantly different to that of normal parenchyma (3.6 ± 0.3, p < 0.0001) and BPH (4.5 ± 1.4, p = 0.0454). Under a cutoff value of 4.2 kPa, a maximum accuracy of 87% was estimated, with a sensitivity of 73%, a specificity of 99%, and an AUC of 0.839 for discriminating PCa from normal parenchyma. Between PCa and BPH, a maximum accuracy of 62%, a sensitivity of 70%, a specificity of 56%, and an AUC of 0.598 were estimated at a 4.5 kPa cutoff. The stiffness values tended to increase as the ISUP grade increased. In conclusion, it is feasible to detect and classify PCa using pelvic MRE. Our observations suggest that MRE could be a supplement to multi-parametric MRI for PCa detection.
Dysbiosis of the Upper Gastrointestinal Tract in Head-and-Neck Cancer Survivors: A Pilot Study Using the Capsule Sponge Device
Authors: Zeber-Lubecka Natalia , Kulecka Maria , Dabrowska Michalina , Kluska Anna , Piątkowska Magdalena , Turkot Maryla Helena , Pilonis Nastazja Dagny , Yusuf Aisha , Nowicki-Osuch Karol , Mikula Michal , Ostrowski Jerzy
Published: October 2024

Background: A non-endoscopic capsule-sponge device allows sampling the entire length of the esophagus. Here, we compared microbiomes of the oral cavity, esophagus, and gastric corpus collected by oral swab, capsule-sponge device, and endoscopic biopsy, respectively, in patients representing three distinct risk profiles for esophageal squamous cell carcinoma (ESCC). Methods: The study enrolled 11 patients with esophageal squamous intraepithelial neoplasia, 21 patients after curative treatment for head and neck squamous cell cancer (HNSCC) (HNSCC survivors), and 40 patients with functional dyspeptic (FD) symptoms. Microbial genomic DNA was analyzed using 16S rRNA gene amplicon sequencing. Results: The Shannon index of the capsule-sponge sample microbiota was significantly higher in FD group than in patients after treatment for HNSCC, and the Chao index of gastric samples differed between HNSCC survivors and FD patients. Analysis of the β-diversity of FD patients, HNSCC, and esophageal squamous intraepithelial neoplasia showed that different genera formed at each location. The abundance of 205, 116, and 9 genera differed between FD patients and HNSCC survivors in the gastric, capsule-sponge, and oral samples, respectively; 33 genera differed between the FD group and patients with esophageal squamous intraepithelial neoplasia in capsule-sponge samples. Conclusions: The bacterial communities of the upper digestive tract were clustered according to the anatomic site. Despite substantial differences in gastric and esophageal microbiota samples between FD patients and HNSCC survivors, the microbial members and diversity showed small differences between FD patients and those with esophageal squamous intraepithelial neoplasia. It remains unclear whether gastric and esophageal dysbiosis is associated with or is a consequence of treatment for HNSCC.
Risk-Stratified Radiotherapy in Pediatric Cancer
Authors: Upadhyay Rituraj , Paulino Arnold C.
Published: October 2024

While the cure rate of cancer in children has markedly improved in the last few decades, late effects continue to be a problem in survivors. Radiotherapy, which is a major component of treatment in many cancers, is one of the major agents responsible for late toxicity. In the past decade, radiotherapy has been omitted in patients achieving excellent response to chemotherapy, such as in Hodgkin lymphoma and some Wilms tumors with lung metastases. Likewise, response to chemotherapy has been used to determine whether lower doses of radiation can be delivered in intracranial germinoma and pediatric nasopharyngeal carcinoma. Molecular subtyping in medulloblastoma is currently being employed, and in WNT-pathway M0 tumors, the reduction in radiotherapy dose to the craniospinal axis and tumor bed is currently being investigated. Finally, dose escalation was recently evaluated in patients with rhabdomyosarcoma > 5 cm who do not achieve a complete response to initial 9 weeks of chemotherapy as well as for unresectable Ewing sarcoma patients to improve local control.
Analysis of Molecular Imaging Biomarkers Derived from [18F]FDG PET/CT in mCRPC: Whole-Body Total Lesion Glycolysis (TLG) Predicts Overall Survival in Patients Undergoing [225Ac]Ac-PSMA-617-Augmented [177Lu]Lu-PSMA-617 Radioligand Therapy
Authors: Burgard Caroline , Khreish Fadi , Dahlmanns Lukas , Blickle Arne , Bastian Moritz B. , Speicher Tilman , Maus Stephan , Schaefer-Schuler Andrea , Bartholomä Mark , Petto Sven , Ezziddin Samer , Rosar Florian
Published: October 2024

Background/Objectives: The augmentation of [177Lu]Lu-PSMA-617 radioligand therapy by alpha emitting [225Ac]Ac-PSMA-617, known as the tandem therapy concept, is a promising escalating treatment option in advanced mCRPC. In this study, we evaluated the value of [18F]FDG PET/CT-derived molecular imaging biomarkers for predicting response and outcome to PSMA tandem RLT in n = 33 patients with insufficient response on [177Lu]Lu-PSMA-617 monotherapy. Methods: Six different molecular imaging parameters at baseline, i.e., before initiation of PSMA tandem RLT with respect to SUVmax, SUVpeak, SUV5, SUVmean, metabolic tumor volume (MTV), and total lesion glycolysis (TLG) were tested for association with response and overall survival (OS). Results: After the initiation of augmentation, 24.2% of patients with a previously insufficient response experienced partial remission, and 39.4% experienced stable disease. The median OS was 7 months (95% CI: 4–11 months). None of the tested parameters were able to predict the response (all p > 0.529). In contrast, the [18F]FDG PET/CT-derived whole-body molecular imaging parameter TLG was significantly (p = 0.029) associated with OS of patients undergoing [225Ac]Ac-PSMA-617 augmented [177Lu]Lu-PSMA-617 RLT after insufficient response to [177Lu]Lu-PSMA-617 monotherapy. Conclusion: Implementing [18F]FDG PET/CT in the management of PSMA-RLT in clinical practice may contribute to outcome prediction and provide a route to more individualized management in mCRPC.
Concomitant Cervical and Anal Screening for Human Papilloma Virus (HPV): Worth the Effort or a Waste of Time?
Authors: Chilou Camille , Espirito Santo Iolanda , Faes Seraina , St-Amour Pénélope , Jacot-Guillarmod Martine , Pache Basile , Hübner Martin , Hahnloser Dieter , Grass Fabian
Published: October 2024

Background: This study represents a follow-up analysis of the AnusGynecology (ANGY) study. Methods: This prospective, cross-sectional, single-center study recruited women for concomitant cervical and anal screening of HPV genotypes and cytology during a single appointment. All women with findings of either HPV or any type of dysplastic lesions on anal smears were offered follow-up in a specialized high-resolution anoscopy (HRA) outpatient clinic, representing the study cohort for this follow-up study. Results: Overall, 275 patients (mean age 42 ± 12) were included. Among them, 102 (37%) had cervical high-risk (HR) HPV. In total, HPV was (incidentally) revealed in 91 patients (33%) on anal smears, while any degree of anal squamous intraepithelial lesion (SIL) was found in 30 patients (11%), 6 if which were high-grade SIL (H-SIL). Furthermore, 10 out of 19 biopsies were positive (3 H-SIL lesions). Only half (48/91, 53%) of the women agreed to undergo the recommended specialized follow-up evaluation. Of them, 18 (38%) were diagnosed with dysplastic lesions (9 low grade (L-SIL) and 9 H-SIL, respectively) on biopsies, while the remaining visits revealed no abnormalities. Multivariable analysis revealed cervical HR-HPV infection (OR 4, 95% CI 2.2–7.5) and anal intercourse (OR 3.1, 95% CI 1.7–5.9) as independent risk factors for anal HR-HPV infection. Conclusions: Close follow-up of these women is hence strongly recommended.
A TRilogy of ATR’s Non-Canonical Roles Throughout the Cell Cycle and Its Relation to Cancer
Authors: Joo Yoon Ki , Ramirez Carlos , Kabeche Lilian
Published: October 2024

Ataxia Telangiectasia and Rad3-related protein (ATR) is an apical kinase of the DNA Damage Response (DDR) pathway responsible for detecting and resolving damaged DNA. Because cancer cells depend heavily on the DNA damage checkpoint for their unchecked proliferation and propagation, ATR has gained enormous popularity as a cancer therapy target in recent decades. Yet, ATR inhibitors have not been the silver bullets as anticipated, with clinical trials demonstrating toxicity and mixed efficacy. To investigate whether the toxicity and mixed efficacy of ATR inhibitors arise from their off-target effects related to ATR’s multiple roles within and outside the DDR pathway, we have analyzed recently published studies on ATR’s non-canonical roles. Recent studies have elucidated that ATR plays a wide role throughout the cell cycle that is separate from its function in the DDR. This includes maintaining nuclear membrane integrity, detecting mechanical forces, and promoting faithful chromosome segregation during mitosis. In this review, we summarize the canonical, DDR-related roles of ATR and also focus on the non-canonical, multifaceted roles of ATR throughout the cell cycle and their clinical relevance. Through this summary, we also address the need for re-assessing clinical strategies targeting ATR as a cancer therapy based on these newly discovered roles for ATR.
Functional Targets for Epstein-Barr Virus BART MicroRNAs in B Cell Lymphomas
Authors: Fachko Devin N. , Goff Bonnie , Chen Yan , Skalsky Rebecca L.
Published: October 2024

MicroRNAs are key post-transcriptional regulators of gene expression and their dysregulation is often linked to cancer. Epstein-Barr virus encodes 22 BamHI A Rightward Transcript (BART) miRNAs, which are expressed in nearly all EBV-associated cancers and implicated in viral pathogenesis. To investigate biological targets for BART miRNAs in B cell lymphomas, we performed a meta-analysis of publicly available Ago-CLIP datasets from EBV-positive Burkitt lymphomas (BLs), primary effusion lymphomas (PELs), AIDS-associated diffuse large B cell lymphomas (DLBCLs), and lymphoblastoid cell lines (LCLs). Our analysis focused on comparing targets of EBV BART miRNAs across the different types of transformed B cells. Using reporter assays, we then experimentally validated over 50 functional interactions between BART miRNAs and cellular protein-coding transcripts involved in activities such as B cell differentiation (PRDM1, IRF4, and MYC), cell cycle regulation (UHMK1, CDKN1A, MDM2, and NPAT), apoptosis (MCL1), signaling and intracellular trafficking (GAB1, SOS1, MAPK1, RAB11A, CAV1, and RANBP9), and tumor suppression (CCDC6). Moreover, ectopic BART miRNA expression in several EBV-negative BL cells induced transcriptional changes that may influence molecular signatures of EBV-associated BLs. Collectively, our findings reveal novel, functional interactions for BART miRNAs in lymphomas and provide insights into their roles in these B cell cancers.
Caseload per Year in Robotic-Assisted Minimally Invasive Esophagectomy: A Narrative Review
Authors: Büdeyri Ibrahim , El-Sourani Nader , Eichelmann Ann-Kathrin , Merten Jennifer , Juratli Mazen A. , Pascher Andreas , Hoelzen Jens P.
Published: October 2024

Esophageal surgery is deemed one of the most complex visceral operations. There is a well-documented correlation between higher caseload and better outcomes, with hospitals that perform more surgeries experiencing significantly lower mortality rates. The approach to caseload per year varies across different countries within Europe. Germany increased the minimum annual required caseload of complex esophageal surgeries from 10 to 26 starting in 2023. Furthermore, the new regulations present challenges for surgical training and staff recruitment, risking the further fragmentation of training programs. Enhanced regional cooperation is proposed as a solution to ensure comprehensive training. This review explores the benefits of robotic-assisted minimally invasive esophagectomy (RAMIE) in improving surgical precision and patient outcomes and aims to evaluate how the caseload per year influences the quality of patient care and the efficacy of surgical training, especially with the integration of advanced robotic techniques.
Emerging Paradigms in Cancer Metastasis: Ghost Mitochondria, Vasculogenic Mimicry, and Polyploid Giant Cancer Cells
Authors: Krotofil Mateusz , Tota Maciej , Siednienko Jakub , Donizy Piotr
Published: October 2024

The capacity of cancer cells to migrate from a primary tumor, disseminate throughout the body, and eventually establish secondary tumors is a fundamental aspect of metastasis. A detailed understanding of the cellular and molecular mechanisms underpinning this multifaceted process would facilitate the rational development of therapies aimed at treating metastatic disease. Although various hypotheses and models have been proposed, no single concept fully explains the mechanism of metastasis or integrates all observations and experimental findings. Recent advancements in metastasis research have refined existing theories and introduced new ones. This review evaluates several novel/emerging theories, focusing on ghost mitochondria (GM), vasculogenic mimicry (VM), and polyploid giant cancer cells (PGCCs).
Artificial Intelligence and Colposcopy: Automatic Identification of Vaginal Squamous Cell Carcinoma Precursors
Authors: Mascarenhas Miguel , Alencoão Inês , Carinhas Maria João , Martins Miguel , Ribeiro Tiago , Mendes Francisco , Cardoso Pedro , Almeida Maria João , Mota Joana , Fernandes Joana , Ferreira João , Macedo Guilherme , Mascarenhas Teresa , Zulmira Rosa
Published: October 2024

Background/Objectives: While human papillomavirus (HPV) is well known for its role in cervical cancer, it also affects vaginal cancers. Although colposcopy offers a comprehensive examination of the female genital tract, its diagnostic accuracy remains suboptimal. Integrating artificial intelligence (AI) could enhance the cost-effectiveness of colposcopy, but no AI models specifically differentiate low-grade (LSILs) and high-grade (HSILs) squamous intraepithelial lesions in the vagina. This study aims to develop and validate an AI model for the differentiation of HPV-associated dysplastic lesions in this region. Methods: A convolutional neural network (CNN) model was developed to differentiate HSILs from LSILs in vaginoscopy (during colposcopy) still images. The AI model was developed on a dataset of 57,250 frames (90% training/validation [including a 5-fold cross-validation] and 10% testing) obtained from 71 procedures. The model was evaluated based on its sensitivity, specificity, accuracy and area under the receiver operating curve (AUROC). Results: For HSIL/LSIL differentiation in the vagina, during the training/validation phase, the CNN demonstrated a mean sensitivity, specificity and accuracy of 98.7% (IC95% 96.7–100.0%), 99.1% (IC95% 98.1–100.0%), and 98.9% (IC95% 97.9–99.8%), respectively. The mean AUROC was 0.990 ± 0.004. During testing phase, the sensitivity was 99.6% and 99.7% for both specificity and accuracy. Conclusions: This is the first globally developed AI model capable of HSIL/LSIL differentiation in the vaginal region, demonstrating high and robust performance metrics. Its effective application paves the way for AI-powered colposcopic assessment across the entire female genital tract, offering a significant advancement in women’s healthcare worldwide.
Tumor Immune Microenvironment in Intrahepatic Cholangiocarcinoma: Regulatory Mechanisms, Functions, and Therapeutic Implications
Authors: Ricci Angela Dalia , Rizzo Alessandro , Schirizzi Annalisa , D’Alessandro Rosalba , Frega Giorgio , Brandi Giovanni , Shahini Endrit , Cozzolongo Raffaele , Lotesoriere Claudio , Giannelli Gianluigi
Published: October 2024

Treatment options for intrahepatic cholangiocarcinoma (iCCA), a highly malignant tumor with poor prognosis, are limited. Recent developments in immunotherapy and immune checkpoint inhibitors (ICIs) have offered new hope for treating iCCA. However, several issues remain, including the identification of reliable biomarkers of response to ICIs and immune-based combinations. Tumor immune microenvironment (TIME) of these hepatobiliary tumors has been evaluated and is under assessment in this setting in order to boost the efficacy of ICIs and to convert these immunologically “cold” tumors to “hot” tumors. Herein, the review TIME of ICCA and its critical function in immunotherapy. Moreover, this paper also discusses potential avenues for future research, including novel targets for immunotherapy and emerging treatment plans aimed to increase the effectiveness of immunotherapy and survival rates for iCCA patients.
Gastrointestinal Microbiota in Gastric Cancer: Potential Mechanisms and Clinical Applications—A Literature Review
Authors: Wu Mengjiao , Tian Chenjun , Zou Zhenwei , Jin Min , Liu Hongli
Published: October 2024

Emerging evidence highlights the crucial role of gastrointestinal microbiota in the pathogenesis of gastric cancer. Helicobacter pylori (H. pylori) infection stands out as a primary pathogenic factor. However, interventions such as anti-H. pylori therapy, gastric surgeries, immunotherapy, and chronic inflammation significantly remodel the gastric microbiome, implicating a broader spectrum of microorganisms in cancer development. These microbial populations can modulate gastric carcinogenesis through various mechanisms, including sustained chronic inflammation, bacterial genotoxins, alterations in short-chain fatty acids, elevated gastrointestinal bile acids, impaired mucus barrier function, and increased concentrations of N-nitrosamines and lactic acid. The dynamic changes in gut microbiota also critically influence the outcomes of anti-cancer therapies by modifying drug bioavailability and metabolism, thus affecting therapeutic efficacy and side effect profiles. Additionally, the effectiveness of radiotherapy can be significantly impacted by gut microbiota alterations. Novel therapeutic strategies targeting the microbiome, such as dietary interventions, probiotic and synbiotic supplementation, and fecal microbiota transplantation, are showing promise in cancer treatment. Understanding the intricate relationship between the gut microbiota and gastric cancer is essential for developing new, evidence-based approaches to the prevention and treatment of this malignancy.
The Utilization of PRAME in the Diagnosis, Prognosis, and Treatment of Melanoma
Authors: Blount Samuel L. , Liu Xiaochen , McBride Jeffrey D.
Published: October 2024

Melanoma, a deadly form of skin cancer, has seen improved survival rates due to advances in diagnosis and treatment, yet the need for further improvement remains critical. Tumor-associated antigens, such as PRAME (Preferentially Expressed Antigen in Melanoma), offer promising avenues for enhanced diagnostic precision, prognostic assessment, and targeted immunotherapy. PRAME, a cancer testis antigen, is selectively expressed in various cancers, including melanoma, and plays a key role in promoting tumorigenesis through inhibition of retinoic acid signaling, epithelial-to-mesenchymal transition, and immune evasion. This review explores the diagnostic utility of PRAME in distinguishing melanoma from benign nevi, its prognostic value in aggressive melanoma subtypes, and its potential as a therapeutic target in cancer vaccines and adoptive T-cell therapies. While PRAME-targeted therapies face challenges such as tumor heterogeneity and immune suppression, ongoing research aims to overcome these barriers, offering hope for more effective melanoma treatments.
The Effects of the COVID-19 Pandemic on Pediatric Orthopedic Injuries: A Single-Center Retrospective Study
Authors: Hinckel Betina , Hasan Sazid , Yin Clark , Lau Jimmy , Saleh Saeed , Saleh Ehab
Published: October 2024

Objectives: The COVID-19 pandemic has had a profound effect on the pediatric population worldwide. The consequences of the lockdown and the reallocation of hospital resources have caused notable changes in the presentation of pediatric orthopedic injuries. Through our use of patient records, we were able to display the epidemiological trends, as well as analyze the changes in the type and mechanism of various injuries. Our purpose is to further understand the considerable effects of the COVID-19 pandemic on pediatric orthopedic trauma and help guide the allocation of future healthcare resources. Methods: We conducted a retrospective cohort study on pediatric patients admitted for any orthopedic injury over a 3-year period (September 2018 to August 2021) at a Midwest level 1 trauma center. Cases were stratified into two groups based on the United States’ COVID-19 lockdown (19 March 2020): Pre-COVID-19 cases were any cases prior to the lockdown and Intra-COVID-19 which are cases following the lockdown. Numerical data and categorical variables were summarized and differences between the case groups were examined using either two-Proportion Z-Test, Independent Two-Sample t-test, Pearson’s chi-squared, or Fisher’s exact tests. Results: A total of 3179 pediatric orthopedic referrals occurred between the Pre-COVID-19 and Intra-COVID-19 study periods. We observed a general decrease in orthopedic injuries following the COVID-19 lockdowns with 1235 injuries compared to 1606 Pre-COVID-19. Patterns in the locations of injuries changed; notably, fractures of the humerus, tibia, and fibula decreased significantly (p < 0.05). Several mechanisms of injuries decreased significantly following the onset of COVID-19 including injuries caused by monkey bars, basketball, and automobiles (p < 0.01). There was a significant increase in the overall injury severity during the Intra-COVID-19 period (p < 0.05). Conclusions: Although there was a reduction in acute orthopedic trauma referrals, many injury mechanisms displayed similar trends regardless of restrictions. We clinically observed an overall increase in the severity of pediatric orthopedic injuries during the COVID-19 pandemic.
Conducting Patient-Oriented Research in Pediatric Populations: A Narrative Review
Authors: Cooper Alan P. , Nguyen Linda , Irelewuyi Oluwapolola , Miller Steven P.
Published: October 2024

It has become increasingly common for researchers to partner with patients as members of the research team and collaborate to use their lived experiences to shape research priorities, interventions, dissemination, and more. The patient-oriented research (POR) model has been adopted by both adult and pediatric health researchers. This cultural change to conducting pediatric health research brings with it new methodologies, tools, challenges, and benefits. In this review, we aim to provide guidance on how to conduct POR for pediatric populations using examples from the literature. We describe considerations for engagement before the project begins, for engagement across the research cycle, and for measurement and evaluation. We aim to show that conducting POR is feasible, beneficial, and that many common challenges and barriers can be overcome with preparation and usage of specific tools.
Evolutionary Mechanism of Solidification Behavior in the Melt Pool During Disk Laser Cladding with 316L Alloy
Authors: Li Chang , Liu Jiabo , Li Shuchao , Kong Fanhong , Wang Xuan , Sun Han , Sun Yichang
Published: October 2024

Laser cladding is an emerging environmentally friendly surface-strengthening technology. During the cladding process, the changes in molten pool temperature and velocity directly affect the solidification process and element distribution. The quantitative revelation of the directional solidification mechanism in the molten pool during the cladding process is crucial for enhancing the quality of the cladding layer. In this study, a multi-field coupling numerical model was developed to simulate the coating process of 316L powder on 45 steel matrices using a disk laser. The instantaneous evolution law of the temperature and flow fields was derived, providing input conditions for simulating microstructure evolution in the molten pool’s paste zone. The behavior characteristics of the molten pool were predicted through numerical simulation, and the microstructure evolution was simulated using the phase field method. The phase field model reveals that dendrite formation in the molten pool follows a sequence of plane crystal growth, cell crystal growth, and columnar crystal growth. The dendrites can undergo splitting to form algal structures under conditions of higher cooling rates and lower temperature gradients. The scanning speed of laser cladding (6 mm/s) has minimal impact on dendrite growth; instead, convection within the molten pool primarily influences dendrite growth and tilt and solute distribution.
Lightweight Mutually Authenticated Key Exchange with Physical Unclonable Functions
Authors: Minwalla Cyrus , Plusquellic Jim , Tsiropoulou Eirini Eleni
Published: October 2024

Authenticated key exchange is desired in scenarios where two participants must exchange sensitive information over an untrusted channel but do not trust each other at the outset of the exchange. As a unique hardware-based random oracle, physical unclonable functions (PUFs) can embed cryptographic hardness and binding properties needed for a secure, interactive authentication system. In this paper, we propose a lightweight protocol, termed PUF-MAKE, to achieve bilateral mutual authentication between two untrusted parties with the help of a trusted server and secure physical devices. At the end of the protocol, both parties are authenticated and possess a shared session key that they can use to encrypt sensitive information over an untrusted channel. The PUF’s underlying entropy hardness characteristics and the key-encryption-key (KEK) primitive act as the root of trust in the protocol’s construction. Other salient properties include a lightweight construction with minimal information stored on each device, a key refresh mechanism to ensure a fresh key is used for every authentication, and robustness against a wide range of attacks. We evaluate the protocol on a set of three FPGAs and a desktop server, with the computational complexity calculated as a function of primitive operations. A composable security model is proposed and analyzed considering a powerful adversary in control of all communications channels. In particular, session key confidentiality is proven through formal verification of the protocol under strong attacker (Dolev-Yao) assumptions, rendering it viable for high-security applications such as digital currency.
Effect of Zn on Microstructure and Wear Resistance of Sn-Based Babbitt Alloy
Authors: Ren Xiaoyan , Chen Huimin , Chang Yuan , Chen Ningning , Shi Zhenghua , Zhang Yougui , Guo Zhiming , Hu Jinzhi
Published: October 2024

Tin-based Babbitt alloys are a widely used bearing bushing material which have good comprehensive properties. However, problems such as high-temperature softening and insufficient bearing capacity occur during their use, so the optimization of tin-based Babbitt alloys has become a research hotspot. In this paper, ZChSnSb11-6 alloy was mainly prepared by the gravity casting method, and different amounts of Zn were added to the alloy (the mass fraction values were 0 wt.%, 0.05 wt.%, 0.1 wt.%, 0.15 wt.%, and 0.2 wt.%, respectively). Through the hardness test, the tensile test, the friction and wear test, and the microstructure observation of the prepared alloy, the influence of Zn on the organization and properties of the ZChSnSb11-6 alloy was analyzed. The results show that the size of the SnSb hard phase changed with the increasing content of Zn. The size of the hard phase of the SnSb tended to increase first and then decrease, and the number of phase particles increased first and then decreased, resulting in changes in performance. Through comparison, it was learned that the addition of Zn can effectively improve the hardness, tensile strength, yield strength, and wear resistance of the alloy, but the elongation rate was reduced. When the Zn content was 0.1 wt.%, the hardness value of the alloy reached the maximum value, 25.82 HB, which increased by 7.3% when compared with the sample without Zn. The hardness of the Zn, 0.15 wt.%, is close to that of the Zn, 0.1 wt.%. Compared to the sample without Zn, the tensile strength and elongation of the alloy were maximized at a Zn content of 0.15 wt.%. Compared to the sample without the Zn, the tensile strength was increased by 21.29%, and the elongation rate was increased by 46%. An analysis showed that the alloy has good comprehensive mechanical properties when the Zn content is 0.15 wt.%.
Effect of Loading Direction on Tensile-Compressive Mechanical Behaviors of Mg-5Zn-2Gd-0.2Zr Alloy with Heterogeneous Grains
Authors: Chen Jieming , Xiao Lei , Wang Xuefang , Li Zhuo , Wang Chen , Wang Bingshu , Chen Junfeng , Liu Pan , Zhang Xinyao
Published: October 2024

The tension-compression yield asymmetry caused by the strengthening of Mg-Zn-Gd-Zr alloy due to extrusion deformation is an important issue that must be addressed in its application. In this study, the effects of loading direction on the tensile and compressive mechanical behaviors of Mg-5Zn-2Gd-0.2Zr alloy were systematically investigated. As the loading angle (the angle between the loading direction and the extrusion direction) increases from 0° to 30°, 45°, 60° and 90°, the tensile yield strength decreases more significantly than the compressive yield strength. Consequently, the tension-compression yield asymmetry is gradually improved. Additionally, the ultimate compressive strength decreases more markedly than the ultimate tensile strength with the increment of the loading angle. In tensile tests conducted at 0°, 30° and 45°, two distinct stages of decreasing strain hardening rates are typically observed. For the 60° and 90° tensile tests, one unusual ascending stage of strain hardening rate is observed. For all compressive tests, three stages of strain hardening are consistently noted; however, the increment in strain hardening rate caused by {10–12} extension twinning decreases with the increasing loading angle. A model combining loading angle and Schmid factor distribution was established. The calculated results indicate that the dominant deformation modes during the yielding process also vary significantly with the loading conditions. This clarification highlights the differences in yield strength variations between tension and compression. Finally, an analysis of the plane trace and crack propagation direction near the fracture surface reveals the fracture mechanisms associated with tensile and compressive tests at different loading directions. This study promotes understanding of the mechanical behaviors of Mg-5Zn-2Gd-0.2Zr alloy under different loading directions, and helps to thoroughly elucidate the anisotropic effects of texture on the mechanical properties of magnesium alloys.
Threshold Switching and Resistive Switching in SnO2-HfO2 Laminated Ultrathin Films
Authors: Kalam Kristjan , Aan Mark-Erik , Merisalu Joonas , Otsus Markus , Ritslaid Peeter , Kukli Kaupo
Published: October 2024

Polycrystalline SnO2-HfO2 nanolaminated thin films were grown by atomic layer deposition (ALD) on SiO2/Si(100) and TiN substrates at 300 °C. The samples, when evaluated electrically, exhibited bipolar resistive switching. The sample object with a stacked oxide layer structure of SnO2 | HfO2 | SnO2 | HfO2 additionally exhibited bidirectional threshold resistive switching properties. The sample with an oxide layer structure of HfO2 | SnO2 | HfO2 displayed bipolar resistive switching with a ratio of high and low resistance states of three orders of magnitude. Endurance tests revealed distinguishable differences between low and high resistance states after 2500 switching cycles.
Vascular Perspectives of the Midfacial Superficial Musculoaponeurotic System
Authors: Hînganu Delia , Hînganu Marius Valeriu , Tamaș Camelia , Costan Victor Vlad , Hristian Liliana , Negru Dragoș , Calistru Anca Elena , Cucu Ramona Paula , Lozneanu Ludmila
Published: October 2024

Objectives: Presently, data on the vascularization of the superficial musculoaponeurotic system of the face (SMAS) are lacking. Thus, the present study aimed to provide new conclusive data about the topography, density, and relationship of the SMAS blood vessels with other components, namely, the fibrous connective tissue and muscles. Methods: The study included a control lot of 42 cases from the archive of the radiology department. In this group, nuclear magnetic resonance angiography (MRA) was performed in order to identify the main sources of vascular supply. In the second group, tissue samples were collected from the midfacial region of 45 patients from the Oro-Maxillo-Facial and Plastic and Reconstructive Surgery clinics of ‘St. Spiridon’ County Clinical Emergency Hospital, Iasi. These patients received surgery for excision of tumoral formations that did not involve SMAS components. These samples underwent micro-CT analysis, hematoxylin and eosin (HE) staining, as well as immunohistochemical (IHC) staining for collagen type III, muscle tissue, and the vascular endothelium. Results: We discovered the particular way in which the SMAS components interrelate with vascularization and the regional differences between them. We have discovered a new vascular network specific to the SMAS, highlighted by both the micro-CT technique and microscopy on slides with special IHC staining. Significant differences were observed in the topographic arrangement, density, and relationships of the microscopic vasculature across midfacial regions. IHC staining provided morphological and functional information about the structure and vascularization of SMAS. Conclusions: The MRA technique could not detect the structural blood vessels of the SMAS and other methods for their in vivo visualization must be sought. The blood vessels of the SMAS mainly follow the topography of the muscle fibers. From the SMAS layer where they are found, the distribution branches reach the stroma of the region and the hypoderm. Our data can contribute to the development of surgical techniques tailored to each individual patient, as well as the enhancement of methods for stimulating cutaneous angiogenesis, improving scarring in this region, and advancing biotissue engineering techniques.
Chronic Diarrhea Owing to Microscopic Colitis: A Cohort Study with Insights into Diagnostic Challenges and Size of the Problem
Authors: Gad Ahmed Ibrahim , Salem Sara Mohamed , Nofal Hanaa A. , Rashed Hayam , Ali Hossam Tharwat , Almadani Noura , Mahfouz Rasha , Ibrahim Nevin F. , Sadek Ayman M. E. M.
Published: October 2024

Background: Microscopic colitis (MC) is a recognized cause of chronic diarrhea and is often underestimated when a colonoscopy appears normal. This study aims to accurately diagnose chronic diarrhea through histopathological examination of colonoscopic mucosal biopsies and assess the prevalence of microscopic colitis and the diagnostic value of biomarkers. Methods: A hospital-based cohort study was conducted on 116 patients with chronic diarrhea. Colonoscopies and colonic mucosal biopsies were performed and analyzed, along with various tests including fecal calprotectin (FC) level, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), stool analysis, routine laboratory tests, and clinical data related to nocturnal diarrhea, abdominal pain, and unexplained weight loss. Results: In the study group, 32.8% had MC, with 25.9% having lymphocytic colitis (LC) and 6.9% having collagenous colitis (CC). Patients with MC had significantly higher FC, ESR, and CRP levels than those without colitis (p < 0.001). Factors associated with MC included nocturnal diarrhea (OR = 4.26; 95% CI [1.64–11.08]; p-value = 0.003) and abdominal pain (OR = 4.62; 95% CI [1.85–11.54]; p-value = 0.001). ESR at a cutoff >14 mm/h and FC at a cutoff >64 mcg/g showed excellent validity in diagnosing MC with area under the curve (AUC) values of 0.94 and 0.97, respectively. Conclusions: Microscopic colitis, particularly LC-type, is not an uncommon cause of chronic diarrhea, especially when accompanied by symptoms such as abdominal pain and nocturnal diarrhea, warranting further investigation, including inflammatory markers and colonic biopsy. Inflammatory markers can be useful in diagnosing MC with proper values and approaches; however, further studies are needed.