bluuebunny/crossref_metadata_2025 huggingface dataset:
GoPerio - impact of a personalized video and an automated two-way text-messaging system in oral hygiene motivation: study protocol for a randomized controlled trial
Authors: Garyga Valentin , Pochelu Florian , Thivichon-Prince Béatrice , Aouini Walid , Santamaria Julie , Lambert France , Maucort-Boulch Delphine , Gueyffier Francois , Gritsch Kerstin , Grosgogeat Brigitte
Published: December 2019

Background

Oral hygiene is of paramount importance for the preservation of oral health, and for patients affected by periodontal disease establishing an effective oral hygiene routine is the first step of therapy. Several clinical frameworks have been developed to foster behavior change, such as motivational interviewing. However, two obstacles can be identified. First, patients tend to forget the advice they were given during the consultation. Second, it is hard to maintain motivation in the long term, thus leading to relapse. An innovative eHealth solution was designed with the aim to tackle both obstacles and supplement the current clinical standard of care. The primary objective is to compare the full mouth plaque scores of study groups (eHealth plus standard of care versus standard of care only) at 8 weeks of follow up. The main secondary objective is to compare the full mouth bleeding score at 8 weeks of follow up.

Methods/design

The “GoPerio” study is a multicenter, randomized, controlled trial assessing the impact of a novel eHealth concept for oral hygiene motivation (personalized video of oral hygiene routine available for the patient via a cloud server plus interactive text messages) in addition to the current standard of care (motivational interviewing plus tooth scaling and polishing). The minimum sample size required is 86 patients. Participants will be randomized (allocation ratio 1:1): test group (eHealth plus standard of care) versus control group (standard of care only). The primary outcome is oral hygiene as measured by the full mouth (six sites per tooth) plaque control record (PCR) index. The main secondary outcome is gingival inflammation as measured by the full mouth (six sites per tooth) bleeding on probing (BOP) index. Both the primary and the main secondary outcomes are evaluated by blinded and calibrated examiners at 8 weeks of follow up. The other secondary outcomes are patient satisfaction and patient behavior change and motivation.

Discussion

The study will investigate the value of an innovative eHealth approach to strengthen patient motivation for oral hygiene. If proven effective, such an approach would supplement the current clinical standard of care, resulting in improved clinical outcomes with negligible impact on productivity in a dental practice.

Trial registration

ClinicalTrials.gov, NCT03109808. Registered on 12 April 2017.

Sponsor: Hospices Civils de Lyon. BP 2251, 3 quai des Célestins, 69,229 Lyon cedex 02.

Protocol version: 1.0 as of 21 September 2016.
Effects of larval diets and temperature regimes on life history traits, energy reserves and temperature tolerance of male Aedes aegypti (Diptera: Culicidae): optimizing rearing techniques for the sterile insect programmes
Authors: Sasmita Hadian Iman , Tu Wu-Chun , Bong Lee-Jin , Neoh Kok-Boon
Published: December 2019

Background

Producing high quality sterile males is vital in Aedes aegypti rear-and-release birth control strategies. Larval diets, rearing temperatures, and their interactions determine the accumulation rates of essential nutrients in larvae, but these factors have been understudied in relation to mass-rearing techniques for producing eminent males.

Methods

We compared the effects of two larval diets, a cereal-legume-based diet (Khan’s diet) and a standard larval diet developed in the FAO/IAEA Insect Pest Control Laboratory (IAEA 2 diet). Diets were tested at selected temperatures for both larval and male adult life history traits, adult extreme temperature tolerance, and mating capacity relative to energy reserves of reared male adult Ae. aegypti.

Results

Khan’s diet resulted in shorter immature development time at each test temperature (except for 25 °C) than an IAEA 2 diet. Larvae reared at 28 °C and 32 °C with Khan’s diet demonstrated low pupation rates (c.80%). We accounted for these phenomena as secondary sex ratio manipulation, because a higher proportion of male adults emerged at 28 °C and 32 °C than that for the IAEA 2 diet. In general, the pupal development time shortened as temperature increased, resulting in higher teneral energy reserves in male mosquitoes. High energy reserves allowed male mosquitoes reared with Khan’s diet to have higher adult longevity (5–6 days longer when sugar-fed and 2–3 days longer when water-fed) and tolerance of heat stress than those fed on the IAEA 2 diet. The IAEA 2 diet produced larger male mosquitoes than Khan’s diet did: mosquitoes fed on Khan’s diet were 1.03–1.05 times smaller than those fed on the IAEA 2 diet at 28 °C and 32 °C. No evidence indicated reduced mating capacity for small mosquitoes fed on Khan’s diet.

Conclusions

Larvae reared at 28 °C and 32 °C with Khan’s diet were characterized by shorter immature development time compared with those fed on the IAEA 2 diet. Adult mosquitoes produced from that larval rearing condition exhibited a significant male bias, long lifespan, and better endurance against extreme temperatures relative to energy reserves. Thus, the larval diet at rearing temperature of 28 °C and 32 °C optimized rearing techniques for the sterile insect programmes. However, mating competitiveness and flight performance of adult males require further investigation.
Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling
Authors: Kong Yuanyuan , Hu Xiaoli , Zhong Yingqun , Xu Ke , Wu Buling , Zheng Jianmao
Published: December 2019

Background

Magnesium (Mg2+)-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells (DPSCs), but the regulatory mechanisms remain undefined. The aim of this work was to assess magnesium’s function in the above process and to explore the associated signaling pathway.

Methods

DPSCs underwent culture in odontogenic medium with the addition of 0, 1, 5, or 10 mM MgCl2. Intracellular Mg2+ levels in DPSCs were evaluated flow cytometrically using Mag-Fluo-4-AM. Mg2+-entry was inhibited by TRPM7 inhibitor 2-aminoethoxydiphenyl borate (2-APB). RNA-Sequencing was carried out for assessing transcriptome alterations in DPSCs during odontogenic differentiation associated with high extracellular Mg2+. KEGG pathway analysis was performed to determine pathways related to the retrieved differentially expressed genes (DEGs). Immunoblot was performed for assessing magnesium’s role and exploring ERK/BMP2/Smads signaling.

Results

Mg2+-enriched microenvironment promoted odontogenic differentiation in DPSCs via intracellular Mg2+ increase. Consistently, the positive effect of high extracellular Mg2+ on odontogenic differentiation in DPSCs was blocked by 2-APB, which reduced Mg2+ entry. RNA-sequencing identified 734 DEGs related to odontogenic differentiation in DPSCs in the presence of high extracellular Mg2+. These DEGs participated in many cascades such as MAPK and TGF-β pathways. Consistently, ERK and BMP2/Smads pathways were activated in DPSCs treated with high extracellular Mg2+. In agreement, ERK signaling inhibition by U0126 blunted the effect of high extracellular Mg2+ on mineralization and odontogenic differentiation in DPSCs. Interestingly, BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly decreased by U0126, indicating that BMP2/Smads acted as downstream of ERK.

Conclusions

Mg2+-enriched microenvironment promotes odontogenic differentiation in DPSCs by activating ERK/BMP2/Smads signaling via intracellular Mg2+ increase. This study revealed that Mg2+-enriched microenvironment could be used as a new strategy for dental pulp regeneration.
Visual contour tracking based on inner-contour model particle filter under complex background
Authors: Cao Songxiao , Wang Xuanyin
Published: December 2019

In this paper, a novel particle filter–based visual contour tracking method is proposed, which uses inner-contour model to track contour object under complex background. The purpose is to achieve effectiveness and robustness against complex background. To that end, the proposed method first utilized Sobel edge detector to detect the edge information along the normal line of the contour. Then, it sampled the inner part of the normal line to get the local color information, which was then combined with the edge information to construct new normal line likelihood. After that, all the inner color information was used to construct global color likelihood. Finally, the edge information, local color information, and global color information were fused into new observation likelihood. Experimental results showed that the proposed method was robust for contours tracking under complex background, and it was also computationally efficient and can run in real-time completely.
Operators constructed by means of basic sequences and nuclear matrices
Authors: Morsy Ahmed , Faried Nashat , Harisa Samy A. , Nisar Kottakkaran Sooppy
Published: December 2019

In this work, we establish an approach to constructing compact operators between arbitrary infinite-dimensional Banach spaces without a Schauder basis. For this purpose, we use a countable number of basic sequences for the sake of verifying the result of Morrell and Retherford. We also use a nuclear operator, represented as an infinite-dimensional matrix defined over the space $\ell _{1}$ℓ1 of all absolutely summable sequences. Examples of nuclear operators over the space $\ell _{1}$ℓ1 are given and used to construct operators over general Banach spaces with specific approximation numbers.
In vitro and in vivo anti-aging effects of compounds isolated from Artemisia iwayomogi
Authors: Kim Kyung Young , Lee Eun Ju , Whang Wan Kyunn , Park Cho Hi
Published: December 2019

In Korean folk medicine, Artemisia iwayomogi has largely been employed for the improvement of diabetic complications and hepatic function as well as in the treatment of female diseases and skin whitening. Accordingly, the present study sought to assess cosmeceutical activity of Artemisia iwayomogi. Through activity-guided fractionation, scopolin (AI-1), 2,4-dihydroxy-6-methoxy-acetophenone 4-O-β-D-glucoside (AI-2), scopoletin (AI-3), kaempferol-3-O-methyl ether (AI-4) and luteolin (AI-5) were isolated from ethyl acetate and water fractions of Artemisia iwayomogi. Of them, AI-4 and AI-5 exhibited strong antioxidative and whitening effects. In addition, AI-1, AI-3, AI-4, and AI-5 demonstrated considerable anti-wrinkle performances. According to results of quantitative analysis of compounds isolated from Artemisia iwayomogi, scopolin (AI-1) is a major component of Artemisia iwayomogi. Moreover, O/W type cream made by water fraction which included scopolin showed unwrinkling effect for 8 weeks when it was applied to face in an in vivo study. Specific contributions of Artemisia iwayomogi were as follows: AI-1, 1.21 mg/g; AI-2, 0.26 mg/g; AI-3, 0.38 mg/g; AI-4, 0.01 mg/g; and AI-5, 0.04 mg/g.
Diurnal variation of salivary oxidative stress marker 8-hydroxyguanine
Authors: Watanabe Sintaroo , Kawasaki Yuya , Kawai Kazuaki
Published: December 2019

Introduction

Oxidative stress is a risk factor for life-style related diseases, including cancer. We recently reported that the oxidative stress marker 8-hydroxyguanine (8-OHGua) can be measured in saliva non-invasively. Understanding the diurnal pattern of salivary 8-OHGua levels is crucial for evaluating the oxidative stress. In this study, we analyzed the diurnal variation of salivary 8-OHGua levels.

Findings

The salivary 8-OHGua levels were relatively stable in the daytime (10:00–22:00). The daytime 8-OHGua levels seemed to represent the individual oxidative stress status. The average amount and the variation of the salivary 8-OHGua levels immediately after awakening were higher than those of the daytime levels.

Conclusions

The 8-OHGua levels in saliva exhibited diurnal variation. The levels were higher at the time of awakening. At this point, the daytime levels of salivary 8-OHGua may be appropriate for evaluating the individual oxidative stress status. Further study is needed for understanding and utilizing the 8-OHGua levels at the time of awakening.
The added value of18F-FDG PET/CT in staging non-small cell lung cancer
Authors: Sheha Aliaa S. , Elia Remon Zaher , Ghoneim Nada Mohammed Farid Hassan
Published: December 2019

Background

Non-small cell lung cancer (NSCLC) accounts for about 80% of all lung cancers. The current criteria for its staging are based on the TNM system that determines treatment options and predicts survival rate in patients. The aim of the study was to evaluate the diagnostic accuracy of 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography PET/CT in staging of NSCLC patients.

Methods

A retrospective study was conducted. We reviewed the CT and PET/CT examinations done in our institution on pathologically proven patients of NSCLC, in the period between October 2018 and end of July 2019.

Results

A total of 40 patients were evaluated with the age ranging from 37 to 77 years old, and the mean was 55.63 years (SD ± 10.29). There were 31 male cases and 9 female cases. When we compared contrast enhanced CT (CECT) to PET-CT for staging, PET-CT helped upstage disease in 10 of 40 patients (25%) and downstage in 3 of 40 patients (7.5%).

Conclusion

PET/CT is a useful imaging tool in initial staging of the newly diagnosed patients with NSCLC. It is better thаn СT alone fоr detection of malignant lesions for accurate staging. It can change the strategy of treatment according to its findings.
Preoperative MRI of perianal fistula evaluation and its impact on surgical outcome
Authors: Algazzar Hesham Youssef , Eldib Diaa Bakry , Bahram Mahmoud A. , Zaher Nasser A.
Published: December 2019

Background

Perianal fistula (PAF) is an abnormal tract communicating an external cutaneous opening in the perianal region to the anal canal. PAF is one of the common anorectal disorders in surgical practice with high prevalence. The current study aimed to determine the ability of preoperative MRI for preoperative evaluation of perianal fistula.

Results

This is a retrospective evaluation of 65 patients with perianal fistula. MRI fistula imaging-related data were revised, and fistula severity was scored using criteria of both local extension of fistulas and active inflammation for a total maximum score of 22. Preoperative MRI could predict the severity of perianal disease with sensitivity, specificity, and accuracy rates of 75%, 92%, and 84.6%, respectively. Surgical findings concerning PAF severity correlated significantly with MRI findings. Diffusion-weighted magnetic resonance imaging (DW-MRI) provided high sensitivity and accuracy with 100% specificity for fistula visualization and highest sensitivity, specificity, and accuracy for detection of cavities > 3 mm in diameter. DW-MRI provided the highest specificity rate on ROC curve analysis among the three MRI pulse sequences (DW-MRI, short tau inversion recovery (STIR), and T1 post-contrast).

Conclusion

MRI is valuable and accurate for preoperative investigation for PAF evaluation and abscess localization. MRI allowed accurate detection of internal fistula opening and its relation to sphincters. DW-MRI is a valuable sequence with highest diagnostic yield, and its addition to STIR WI improves sensitivity and specificity for determination of fistula activity and extension.
The impact of bubble curtains on seismic air-gun signatures and its high-frequency emission
Authors: Wehner Daniel , Landrø Martin
Published: December 2019

In marine seismic acquisition, air guns are the most common source and, in recent years, research on their impact on the marine environment has increased. The main focus is on the reduction of emitted high frequencies, approximately greater than 200 Hz, which are normally not useful for seismic imaging. Therefore, potential ways to reduce the high frequencies from air guns are investigated and the development of alternative source types has increased. We have investigated the impact of bubble curtains on the source signature from seismic air guns because bubble curtains are known to mitigate high frequencies in other applications, e.g., pile driving for offshore wind farms. We have conducted tank experiments with two different configurations of bubble curtains around a single air gun and compared the results to the conventional source signature without a bubble curtain. The two different bubble curtains vary in size and in the way they are attached to the air gun. The amount of injected air into the bubble curtains is varied for both configurations. We compare the measured results to simulated data using a common model for air-gun source signatures. The results indicate a reduced peak amplitude with increasing air injection through the bubble curtain. This corresponds to a gradually decreasing frequency content for frequencies greater than 50 Hz. The frequencies of the source signal of less than 50 Hz are practically unaffected by the bubble curtain. In addition, the bubble time period of the source signal is slightly increased with an increasing amount of air injection through the bubble curtain. The main cause for the reduced peak amplitude is likely to be a buffer effect of the bubble curtain on the released air. Hence, a bubble curtain concentrated around the air-gun ports could be an efficient and practical solution to reduce the high-frequency acoustic emission from air guns.
Mutation of SGK3, a Novel Regulator of Renal Phosphate Transport, Causes Autosomal Dominant Hypophosphatemic Rickets
Authors: Cebeci Ayşe Nurcan , Zou Minjing , BinEssa Huda A , Alzahrani Ali S , Al-Rijjal Roua A , Al-Enezi Anwar F , Al-Mohanna Futwan A , Cavalier Etienne , Meyer Brian F , Shi Yufei
Published: December 2019

Context

Hypophosphatemic rickets (HR) is a group of rare hereditary renal phosphate wasting disorders caused by mutations in PHEX, FGF23, DMP1, ENPP1, CLCN5, SLC9A3R1, SLC34A1, or SLC34A3.

Objective

A large kindred with 5 HR patients was recruited with dominant inheritance. The study was undertaken to investigate underlying genetic defects in HR patients.

Design

Patients and their family members were initially analyzed for PHEX and FGF23 mutations using polymerase chain reaction sequencing and copy number analysis. Exome sequencing was subsequently performed to identify novel candidate genes.

Results

PHEX and FGF23 mutations were not detected in the patients. No copy number variation was observed in the genome using CytoScan HD array analysis. Mutations in DMP1, ENPP1, CLCN5, SLC9A3R1, SLC34A1, or SLC34A3 were also not found by exome sequencing. A novel c.979–96 T>A mutation in the SGK3 gene was found to be strictly segregated in a heterozygous pattern in patients and was not present in normal family members. The mutation is located 1 bp downstream of a highly conserved adenosine branch point, resulted in exon 13 skipping and in-frame deletion of 29 amino acids, which is part of the protein kinase domain and contains a Thr-320 phosphorylation site that is required for its activation. Protein tertiary structure modelling showed significant structural change in the protein kinase domain following the deletion.

Conclusions

The c.979–96 T>A splice mutation in the SGK3 gene causes exon 13 skipping and deletion of 29 amino acids in the protein kinase domain. The SGK3 mutation may cause autosomal dominant HR.
Tissue transglutaminase 2 regulates tumor cell tensional homeostasis by increasing contractility
Authors: Bordeleau Francois , Wang Wenjun , Simmons Alysha , Antonyak Marc A. , Cerione Richard A. , Reinhart-King Cynthia A.
Published: December 2019

Abnormal tensional cellular homeostasis is now considered a hallmark of cancer. Despite this, the origin of this abnormality remains unclear. In this work, we investigated the role of tissue transglutaminase 2 (TG2, also known as TGM2), a protein associated with poor prognosis and increased metastatic potential, and its relationship to the EGF receptor in the regulation of the mechanical state of tumor cells. Remarkably, we observed a TG2-mediated modulation of focal adhesion composition as well as stiffness-induced FAK activation, which was linked with a distinctive increase in cell contractility, in experiments using both pharmacological and shRNA-based approaches. Additionally, the increased contractility could be reproduced in non-malignant cells upon TG2 expression. Moreover, the increased cell contractility mediated by TG2 was largely due to the loss of EGFR-mediated inhibition of cell contractility. These findings establish intracellular TG2 as a regulator of cellular tensional homeostasis and suggest the existence of signaling switches that control the contribution of growth factor receptors in determining the mechanical state of a cell.
History-dependent perturbation response in limb muscle
Authors: Libby Thomas , Chukwueke Chidinma , Sponberg Simon
Published: December 2019

Muscle mediates movement but movement is typically unsteady and perturbed. Muscle is known to behave non-linearly and with history dependent properties during steady locomotion, but the importance of history dependence in mediating muscles function during perturbations remains less clear. To explore muscle's capacity to mitigate perturbations during locomotion, we constructed a series of perturbations that varied only in kinematic history, keeping instantaneous position, velocity and time from stimulation constant. We find that muscle's perturbation response is profoundly history dependent, varying by four fold as baseline frequency changes, and dissipating energy equivalent to ∼6 times the kinetic energy of all the limbs in 5 ms (nearly 2400 W Kg−1). Muscle's energy dissipation during a perturbation is predicted primarily by the force at the onset of the perturbation. This relationship holds across different frequencies and timings of stimulation. This history dependence behaves like a viscoelastic memory producing perturbation responses that vary with the frequency of the underlying movement.
A new method for mapping spatial resolution in compound eyes suggests two visual streaks in fiddler crabs
Authors: Bagheri Zahra M. , Jessop Anna-Lee , Kato Susumu , Partridge Julian C. , Shaw Jeremy , Ogawa Yuri , Hemmi Jan M.
Published: December 2019

Visual systems play a vital role in guiding the behaviour of animals. Understanding the visual information animals are able to acquire is therefore key to understanding their visually-mediated decision making. Compound eyes, the dominant eye type in arthropods, are inherently low-resolution structures. Their ability to resolve spatial detail depends on sampling resolution (interommatidial angle) and the quality of ommatidial optics. Current techniques for estimating interommatidial angles are difficult, and generally require in vivo measurements. Here, we present a new method for estimating interommatidial angles based on the detailed analysis of 3D Micro-CT images of fixed samples. Using custom-made MATLAB software we determine the optical axes of individual ommatidia and project these axes into the three-dimensional space around the animal. The combined viewing directions of all ommatidia, estimated from geometrical optics, allow us to estimate interommatidial angles and map the animal's sampling resolution across its entire visual field. The resulting topographic representations of visual acuity match very closely the previously published data obtained from both fiddler and grapsid crabs. However, the new method provides additional detail that was not previously detectable and reveals that fiddler crabs, rather than having a single horizontal visual streak as is common in flat world inhabitants, likely have two parallel streaks located just above and below the visual horizon. A key advantage of our approach is that it can be used on appropriately preserved specimens allowing the technique to be applied to animals such as deep-sea crustaceans that are inaccessible or unsuitable for in vivo approaches.
A selfish genetic element linked to increased lifespan impacts metabolism in female house mice
Authors: Lopes Patricia C. , Lindholm Anna K.
Published: December 2019

Gene drive systems can lead to the evolution of traits that further enhance the transmission of the driving element. In gene drive, one allele is transmitted to offspring at a higher frequency than the homologous allele. This has a range of consequences, which generally include a reduction in fitness of the carrier of the driving allele, making such systems “selfish”. The t haplotype is one such driver, found in house mice. It is linked to a reduction in litter size in matings among heterozygous animals, but also to increased lifespan in wild females that carry it. Here, we tested whether carrying the t haplotype was associated with altered resting metabolic rate (RMR). We show that females carrying the t haplotype decrease RMR as they increase in size, compared to wildtype females or males of either genotype. Our study elucidates a plausible mechanism by which a selfish genetic element increases lifespan.
Kinetic volume analysis on dynamic contrast-enhanced MRI of triple-negative breast cancer: associations with survival outcomes
Authors: Hayashi Yoko , Satake Hiroko , Ishigaki Satoko , Ito Rintaro , Kawamura Mariko , Kawai Hisashi , Iwano Shingo , Naganawa Shinji
Published: December 2019

Objective:

To evaluate the associations between computer-aided diagnosis (CAD)-generated kinetic volume parameters and survival in triple-negative breast cancer (TNBC) patients.

Methods:

40 patients with TNBC who underwent pre-operative MRI between March 2008 and March 2014 were included. We analyzed CAD-generated parameters on dynamic contrast-enhanced MRI, visual MRI assessment, and histopathological data. Cox proportional hazards models were used to determine associations with survival outcomes.

Results:

12 of the 40 (30.0%) patients experienced recurrence and 7 died of breast cancer after a median follow-up of 73.6 months. In multivariate analysis, higher percentage volume (%V) with more than 200% initial enhancement rate correlated with worse disease-specific survival (hazard ratio, 1.12; 95% confidence interval, 1.02–1.22; p-value, 0.014) and higher %V with more than 100% initial enhancement rate followed by persistent curve type at 30% threshold correlated with worse disease-specific survival (hazard ratio, 1.33; 95% confidence interval, 1.10–1.61; p-value, 0.004) and disease-free survival (hazard ratio, 1.27; 95% confidence interval, 1.12–1.43; p-value, 0.000).

Conclusion:

CAD-generated kinetic volume parameters may correlate with survival in TNBC patients. Further study would be necessary to validate our results on larger cohorts.

Advances in knowledge:

CAD generated kinetic volume parameters on breast MRI can predict recurrence and survival outcome of patients in TNBC. Varying the enhancement threshold improved the predictive performance of CAD generated kinetic volume parameter.
Impact of pulmonary rehabilitation programs in improving health status in COPD patients
Authors: TUDORACHE Emanuela , MOTOC Nicoleta Stefania , PESCARU Camelia , CRISAN Alexandru , CIUMARNEAN Lorena
Published: December 2019

Pulmonary rehabilitation programme (PRP) have a positive impact on multiple outcomes of COPD, such as decreasing symptoms, increasing exercise tolerance and improving general health status. The aim of this study is to evaluate exercise tolerance impairment and to assess the impact of PRP in improving health status in patients diagnosed with COPD. It was conducted a prospective parallel group study in the Pulmonary Rehabilitation Department of the Clinical Hospital "V. Babes”, Timisoara, from 2007 to 2010. The subjects included in the study were patients diagnosed with COPD stages I-IV GOLD, initially evaluated and started a PRP, then re-evaluated after 3 weeks and 6 months. The study group included 168 patients, 158 men, mean age 61.73 years. The initial evaluation revealed higher values of dyspnea scores using mMRC scale in advanced COPD stages (3.69±0.77 in patients with COPD stage IV, vs 0.88±0.5 in patients with COPD stage, p<0.05), decreased Forced expiratory volume in 1 second (FEV1), PImax and PEmax, and 6 minutes walking distance values corresponding with COPD severity stages. Re-evaluation at 3 weeks and 6 months after the pulmonary rehabilitation programme was applied showed significant improved dyspnea scores and exercise tolerance. The results of this study reconfirmed the positive and persistent impact of pulmonary rehabilitation programme on muscle dysfunction, dyspnea, and quality of life in COPD patients, regardless of severity. Key words: COPD, pulmonary rehabilitation programme, 6 minutes walking test, pedometry,
Clinical, paraclinical aspects and complex therapeutical approaches in a patient with incomplete paraplegia, post thoracic menigioma surgically treated, in neurofibromatosis context
Authors: SPÎNU Aura , ANDONE Ioana , CHIPĂRUȘ Carmen , FRUNZA Andreea , STOICA Simona , ONOSE Liliana , PATRAȘCU George , ONOSE Gelu
Published: December 2019

Introduction: Neurofibromatosis - type 1 (NF1) and type 2 (NF2) - are genetic disorders of the nervous system that can affect the growth and development of nerve cell tissue and so can determine severe or rather permanent sequels. NF2 implies usually multiple tumors on the cranial and spinal nerves and it is less common than NF1. The most frequent symptom of NF2 is hearing progressive loss, as a consequence of auditory nerves affection and appears at early ages or at twenties. The evolution of a patient with NF2 depends on the number and location of tumors and some of them might develop a life-threatening or disabling condition. With a prompt diagnosis and an appropriate therapy it can be improved the patient prognosis and QOL.
Materials and Methods: This paper presents the case of a 43-year-old patient, with personal antecedents of hearing dysfunction, diagnosed in 2013 with neurofibromatosis that was hospitalized at the IV Neurosurgery Clinic of TEHBA in January 2019 and suffered a re-intervention for removal of the spinal cord tumor (psammomatous meningioma) and with spinal cord decompression. In our clinic, the patient was admitted for incomplete AIS/Frankel C paraplegia, he had initially followed a complex nursing program and subsequently a rehabilitation adequate program. The patient was assessed functionally using the following scales: AIS / Frankel, modified Ashworth, Functional Independence Measure (FIM), Life Quality Assessment (QOL), FAC International Scale, Independence Assessment Scale in Daily Activities (ADL / IADL), Walking Scale for Spinal Cord Injury (WISCI).
Results: The paraclinical assessments (cerebral and spinal cord MRI) detect multiple cerebral tumors and micro-nodules adjacent to the lumbar spinal roots, which, associated with the bilateral acoustic neurinoma (diagnosed in 2013), contributed to the suspicion of the NF2 diagnosis. The patient had two admissions in our clinic division, benefited from a complex neuro-muscular rehabilitation program, having a favourable evolution, with an increase in the evaluated scales scores, now performing walking with a support from another person in walking frame, as well as sphincter re-education, with the neurogenic bladder remission.
Conclusions: Even if there is no cure for neurofibromatosis and no standard treatment, it is important to promptly diagnose such a rare disease and to give an adequate treatment (AINS or other analgesic drugs, surgery, chemotherapy or radiation –when it’s needed, or psychotherapy) for controlling symptoms and also a personalized rehabilitation program (including nursing measures) enhancing including patient's quality of life.

Key words: paraplegia, neurofibromatosis, neuro-muscular rehabilitation,
Effects of diet consistency on mandibular growth. A review
Authors: TSOLAKIS I.A. , VERIKOKOS C. , PERREA D. , BITSANIS E. , TSOLAKIS A.I.
Published: December 2019

This article is a review that focuses on the diet consistency and how this affects mandibular morphology. Various published studies focused on the relationship between mastication and growth of the mandible because it is considered that mandibular growth is dependent on the loads exerted by the function of the masticatory muscles. Moreover it has been pointed out that the increase of orthodontic anomalies is due to the modern softer diet. Even in rats, soft diet is one of the factors causing malocclusions. All of the studies have been experimental, mainly in rodents, since this research is impossible to be applied on humans in a short period of time. Most experimental studies suggested that occlusal loading affects bone mass, bone amount, bone density, the length and the width of the bone, the degree of mineralization, the genetic expression, the collagen immunoreaction and the chondrocytes action on the cartilage. It is stated that bone volumes and thickness of the mandible of rats fed with soft diet were smaller when compared to animals fed with hard diet. Also the mandibles and condyles were smaller and less dense in the rats of soft diet as compared to controls. Furthermore the length and the width of the condyle in the soft diet group of animals were smaller as compared to the condyle of the hard diet group of animals. Soft diets affect also the degree of mineralization, and the action of the chondrocytes on the cartilage.
Seasonal variation of fatty acids composition of milk from grazing ewes in Thessaly, central Greece
Authors: GOVARI M. , ILIADIS S. , PAPAGEORGIOU D. , FLETOURIS D.
Published: December 2019

The aim of this work was to evaluate the changes in fatty acids (FAs) profile and conjugated linoleic acid (CLA) concentration of milk from grazing ewes in winter (December and January) and spring (April and May) in Thessaly, central Greece. No significant changes (P>0.05) in the physicochemical properties (pH and protein, lactose and total solids content) of winter and spring milk were observed. However, the fat content of spring milk was lower (P<0.05) than the winter milk. The saturated FAs content of milk was not significantly changed (P>0.05) during winter neither during spring, whereas the polyunsaturated FAs content was significantly changed (P<0.05) in each of the four months examined. Nevertheless, in the ovine milk of spring, the saturated FAs content was significantly decreased (P<0.05), but the monounsaturated and polyunsaturated FAs content was significantly increased (P<0.05) as compared to that of winter milk. In contrast to the saturated FAs decrease in spring milk, the saturated stearic acid (C18:0) content showed a significant increase (P<0.05) in the spring milk as compared to winter milk. In winter milk, the C18:2 cis-9, trans-11 CLA levels were 0.89±0.05 and 0.98±0.03g/100 g Fatty Acid Methyl Esters (FAMEs) in December and January, respectively, whereas, in spring milk, the CLA levels were significantly increased (P<0.05) to 1.36±0.04 and 1.27±0.03g/100 g FAMEs in April and May, respectively. The atherogenicity index (AI) associated with proatherogenic and antiatherogenic FAs was found significantly (P<0.05) lower in spring milk compared to winter milk.
Hematological and histological alterations detected in striped catfish (Pangasius hypophthalmus) exposed to acute copper concentrations
Authors: Küçük Semra
Published: December 2019

In this study, to examine hematological and histological alterations, striped catfish exposed to 0.0, 2.5, 5.0, 10.0 mg L-1 copper concentrations for 48 hours. After that, four fish were selected per aquarium. First 24 and second 24 h, blood samples were taken. And then, their gills, liver, kidney, and spleen samples were collected. The blood samples were analyzed for hematocrit, hemoglobin, number of erythrocytes, MCHC, MCH and MCV. The tissue samples stained with standart tecnique of haematoxylin and eosin for histological assessment. Copper depending on concentrations increase caused some important hematological and histological alterations on striped catfish. Number of erythroryte, hematocrit and hemoglobin of striped catfish exposed to ≥ 2.5 mg L-1 of Cu concentrations increased in 48 h. Within first 24 h ≥ 2.5 mg L-1 of Cu caused edema, hyperplasia and fusion in gills. After 48 h, ≥ 2.5 mg L-1 of Cu formed very intense hyperplasia and degeneration of cartilage and epithelium tissue in the gills. Pyknotic nuclei and degenation in liver; necrosis and increase number of hemosterin clustering in spleen; degeneration of renal tubules and black spots in kidney were seen. As a result of that, ≥ 2.5 mg L-1 of Cu caused high mortality and histological disorders within 48 h.
ПЕДАГОГИЧЕСКИЙ ЭКСПЕРИМЕНТ ПО ИНСТИТУЦИОНАЛЬНОМУ ПРОЕКТИРОВАНИЮ: «СТУДЕНЧЕСКИЙ ИНВЕСТИЦИОННЫЙ КЛУБ»
Authors: Yakovlev Artem Konstantinovich
Published: December 2019

Существующая сегодня отрасль венчурного инвестирования РФ полна институциями и инструментами, направленными на генерацию новых проектов; созданы и работают десятки фондов и сотни акселерационных программ. Этого нельзя сказать о механизмах, развивающих навыки венчурного инвестирования, в таких условиях сложно говорить о долгосрочных эффективных перспективах важной части венчурной отрасли – предпосевных (pre-Seed) инвестициях. Данная статья описывает авторский педагогический подход к дизайну образовательного процесса: от разработки имитационной игры «Стартап будильник» до проекта нового элемента венчурной отрасли, в котором студенты становятся активными участниками процесса принятия решения об инвестировании студенческих проектов. Автор показывает, что необходимо и возможно повышение интереса к практико-ориентированным формам образования через принятие студентами ответственности как способности влиять на результат образовательных услуг, приведет к росту эффективности учебного процесса.
'Untruss a Point' - Interiority, Sword Combat, and Gender in The Roaring Girl
Authors: Carter Matthew Charles
Published: December 2019

This article discusses the critical apparatus surrounding Dekker and Middleton’s well-known play The Roaring Girl. While previous discussions of the text have focused mostly on Moll’s cross-dressing, I instead look at Moll’s sword skills to show how the lascivious behaviour of London’s men produces her gender performance, which seems unruly by early modern standards. I also examine other rituals of gender construction that texture previous analyses of Moll.
Review: Derek Dunne, Shakespeare, Revenge Tragedy and Early Modern Law: Vindictive Justice.
Authors: Apolloni Jessica
Published: December 2019

This review considers Derek Dunne's Shakespeare, Revenge Tragedy and Early Modern Law: Vindictive Justice (2016).
Review: Evelyn Tribble, Early Modern Actors & Shakespeare’s Theatre: Thinking with the Body.
Authors: Menzer Paul
Published: December 2019

This review considers Evelyn Tribble's Early Modern Actors & Shakespeare’s Theatre: Thinking with the Body (2017).
Review: Jeffrey S. Doty, Shakespeare, Popularity, and the Public Sphere.
Authors: Smith Emma
Published: December 2019

This review considers Jeffrey S. Doty's Shakespeare, Popularity, and the Public Sphere (2017).
Review: Bill Barclay and David Lindley (eds), Shakespeare, Music and Performance.
Authors: Waghorn Jennifer Claire Moss
Published: December 2019

This review considers Bill Barclay and David Lindley (eds), Shakespeare, Music and Performance (2017).
What Is Different About Digital Strategy? From Quantitative to Qualitative Change
Authors: Adner Ron , Puranam Phanish , Zhu Feng
Published: December 2019

The recent attention paid to the challenge of digital transformation signals an inflection point in the impact of digital technology on the competitive landscape. We suggest that this transition can be understood as a shift from the quantitative advances that have historically characterized digital progress (e.g., Moore’s law, Metcalf’s law) to qualitative changes embodied in three core processes underlying modern digital transformation: representation, connectivity, and aggregation. We consider the implications for firm strategy and raise questions for future strategy research.
Potential of Polymeric Materials (Microcrystalline Cellulose and Cotton Fibers) for the Removal of Anionic Dye from Textile Wastewater through Solid Phase Extraction
Authors: Dar Amara , Safdar Amna , Anwar Jamil
Published: December 2019

Alizarin red S dye is frequently used in textile industry and thus present in large quantities in out-drained industrial effluents, which pollute the sources of fresh water, by hindering the light and affecting the aquatic flora and fauna. Such water when used in irrigation purposes later causes threats for human health and animals life. In this work the dye has been effectively removed by using two polymeric materials i.e. microcrystalline cellulose and cotton fiber by solid phase extraction. Various concerned physical parameters were optimized for maximum adsorption through batch experiments. Kinetics of the adsorption process, thermodynamic studies, isotherm studies and desorption of the dye have also been studied. Qmax values for cotton and microcrystalline cellulose come out to be 6.612 mg/g and 5.573mg/g. Further this extraction proved to be spontaneous and endothermic in nature.
Faktor-Faktor yang Mempengaruhi Kriminalitas di Indonesia Tahun 2011-2016 dengan Regresi Data Panel
Authors: Kosmaryati Kosmaryati , Handayani Chandra Arinda , Isfahani Refinanda Nur , Widodo Edy
Published: December 2019

Criminality in Indonesia is increasing every year, therefore an effort is needed to reduce criminality in Indonesia, one of which can be used by knowing which factors influence the increase of criminality. This paper discusses the factors that influence criminality by using panel data regression analysis. Unemployment, domestic violence cases, narcotics cases, embezzlement cases, and fraud cases have positive effect on the amount of criminality with R2 of 0,85823 or 85,823%.

Keywords : panel regression analysis, crime, Indonesia
Analisis Sentimen Masyarakat terhadap Hasil Quick Count Pemilihan Presiden Indonesia 2019 pada Media Sosial Twitter Menggunakan Metode Naive Bayes Classifier
Authors: Andika Lingga Aji , Azizah Pratiwi Amalia Nur , Respatiwulan Respatiwulan
Published: December 2019

Indonesia is one of the countries that adheres to a democratic system. In the course of a democratic system it is marked by periodic general elections. In 2019 Indonesia held a general election simultaneously to elect the President, DPR, DPRD and DPD. After the election, a lot of opinion arise within the community, including on social media twitter. One of the topics discussed was the results of the quick count of the presidential election. Therefore, a method that can be used to analyze sentiment from the quick count opinion is needed, that is naive Bayes method. The aims of this study are to find the best naive Bayes model and to classify sentiments. The result shows the best accuracy of 82.90% with α = 0.05. The classification obtained is 34.5% (471) positive tweets and 65.5% (895) negative tweets on the results of the quick count.

Keywords : sentiment analysis, naive Bayes classifier, elections, quick count
Revisiting the concept of shared value in developing countries: Towards an organisational framework
Authors: Azmat Fara , Fujimoto Yuka , Subramaniam Nava
Published: December 2019

Purpose
 The purpose of this paper is to offer a greater understanding of creating shared value (CSV) in socioeconomically deprived regions. It aims to develop a normative organisational framework of shared value creation using corporate social responsibility (CSR)
ideologies, to help managers design firm level CSR initiatives for use in socioeconomically deprived regions, where it is needed more.
 Design/methodology/approach
 We conducted an in-depth case study of Company X - an industry leader in the controversial paper and
pulp manufacturing sector in Indonesia - drawing on multiple evidence sources, including interviews with 92 stakeholders and a review of secondary data.
 Findings
 Our study presents a context-embedded organisational CSR framework for companies operating in socioeconomically
deprived regions that signifies the need for normative principles of strategic and philanthropic CSR to work 'in parallel', to create shared value in a real sense to benefit both the businesses and the society. The framework further highlights the need to address certain contextual challenges
that impact on the creation of shared value in socioeconomically deprived regions, including managing stakeholder expectations, regularly communicating with stakeholders, and staff capacity building for reporting on CSR and measuring performance.
 Originality/value

Incorporating perspectives from the Global South that reflect the experiences of a company operating in a developing country, our findings provide a useful addition to the debate on shared value creation in socioeconomically deprived regions. In doing so, our study broadens and shifts the
focus on shared value creation from a managerial perspective or single project perspective to an organisational perspective.
Multiphoton microscopy of the dermoepidermal junction and automated identification of dysplastic tissues with deep learning
Authors: Huttunen Mikko J. , Hristu Radu , Dumitru Adrian , Floroiu Iustin , Costache Mariana , Stanciu Stefan G.
Published: December 2019

Histopathological image analysis performed by a trained expert is currently regarded as the gold-standard for the diagnostics of many pathologies, including cancers. However, such approaches are laborious, time consuming and contain a risk for bias or human error. There is thus a clear need for faster, less intrusive and more accurate diagnostic solutions, requiring also minimal human intervention. Multiphoton microscopy (MPM) can alleviate some of the drawbacks specific to traditional histopathology by exploiting various endogenous optical signals to provide virtual biopsies that reflect the architecture and composition of tissues, both in-vivo or ex-vivo. Here we show that MPM imaging of the dermoepidermal junction (DEJ) in unstained fixed tissues provides useful cues for a histopathologist to identify the onset of non-melanoma skin cancers. Furthermore, we show that MPM images collected on the DEJ, besides being easy to interpret by a trained specialist, can be automatically classified into healthy and dysplastic classes with high precision using a Deep Learning method and existing pre-trained convolutional neural networks. Our results suggest that deep learning enhanced MPM for in-vivo skin cancer screening could facilitate timely diagnosis and intervention, enabling thus more optimal therapeutic approaches.
Shape-dependent infrared reflectance properties of CNT forest metamaterial arrays
Authors: Pander Adam , Takano Keisuke , Hatta Akimitsu , Nakajima Makoto , Furuta Hiroshi
Published: December 2019

In this work, shape-dependent mid-infrared properties of novel split ring resonator (SRR) metamaterials composed of single-walled carbon nanotube (CNT) forest are investigated. The introduction of the gap and dip shape to the closed ring geometry reduced the total reflectance by 15%, due to the generation of circular currents and LC resonances in SRRs. The increase of the SRR height reduced the total IR reflectance by 25%. Unique one-dimensional anisotropic electric and photonic properties of CNTs, combined with an artificial refractive index induced in SRR circuits, will stimulate the development of new optoelectronics applications.