bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative physiological, transcriptomic, and WGCNA analyses reveal the key genes and regulatory pathways associated with drought tolerance in Tartary buckwheat
Authors: Meng Heng-Ling , Sun Pei-Yuan , Wang Jia-Rui , Sun Xiao-Qian , Zheng Chuan-Zhi , Fan Ting , Chen Qing-Fu , Li Hong-You
Published: October 2022

Drought stress is one of the major abiotic stress factors that affect plant growth and crop productivity. Tartary buckwheat is a nutritionally balanced and flavonoid-rich pseudocereal crop and also has strong adaptability to different adverse environments including drought. However, little is known about its drought tolerance mechanism. In this study, we performed comparative physiological and transcriptomic analyses of two contrasting drought-resistant Tartary buckwheat genotypes under nature drought treatment in the reproductive stage. Under drought stress, the drought-tolerant genotype XZSN had significantly higher contents of relative water, proline, and soluble sugar, as well as lower relative electrolyte leakage in the leaves than the drought-susceptible LK3. A total of 5,058 (2,165 upregulated and 2,893 downregulated) and 5,182 (2,358 upregulated and 2,824 downregulated) potential drought-responsive genes were identified in XZSN and LK3 by transcriptome sequencing analysis, respectively. Among the potential drought-responsive genes of XZSN, 1,206 and 1,274 genes were identified to be potential positive and negative contributors for XZSN having higher drought resistance ability than LK3. Furthermore, 851 out of 1,206 positive drought-resistant genes were further identified to be the core drought-resistant genes of XZSN based on WGCNA analysis, and most of them were induced earlier and quicker by drought stress than those in LK3. Functional annotation of the 851 core drought-resistant genes found that a large number of stress-responsive genes were involved in TFs, abscisic acid (ABA) biosynthesis, signal transduction and response, non-ABA signal molecule biosynthesis, water holding, oxygen species scavenging, osmotic adjustment, cell damage prevention, and so on. Transcriptional regulatory network analyses identified the potential regulators of these drought-resistant functional genes and found that the HD-ZIP and MYB TFs might be the key downstream TFs of drought resistance in Tartary buckwheat. Taken together, these results indicated that the XZSN genotype was more drought-tolerant than the LK3 genotype as evidenced by triggering the rapid and dramatic transcriptional reprogramming of drought-resistant genes to reduce water loss, prevent cell damage, and so on. This research expands our current understanding of the drought tolerance mechanisms of Tartary buckwheat and provides important information for its further drought resistance research and variety breeding.
Construction of a high-density genetic map and localization of grazing-tolerant QTLs in Medicago falcata L.
Authors: Zhou Xinyue , Li Xiaojie , Zhang Xiaoming , Yin Dabao , Wang Junjie , Zhao Yan
Published: October 2022

Background

Using genomic DNA from 79 F1 plants resulted from a crossing between parents with strong and weak grazing tolerance inMedicago falcataL., we generated anEcoRIrestriction site-associated DNA (RAD) sequencing library. After sequencing and assembly, a high-density genetic map with high-quality SNP markers was constructed, with a total length of 1312.238 cM and an average density of 0.844 SNP/cM.

Methods

The phenotypic traits of 79 F1 families were observed and the QTLS of 6 traits were analyzed by interval mapping.

Results

Sixty three QTLs were identified for seven traits with LOD values from 3 to 6 and the contribution rates from 15% to 30%. Among the 63 QTLs, 17 were for natural shoot height, 12 for rhizome Length, 10 for Shoot canopy diameter, 9 for Basal plant diameter, 6 for stem number, 5 for absolute shoot height, and 4 for rhizome width. These QTLs were concentrated on LG2, LG4, LG5, LG7, and LG8. LG6 had only 6 QTLs. According to the results of QTL mapping, comparison of reference genomes, and functional annotation, 10 candidate genes that may be related to grazing tolerance were screened. qRT-PCR analysis showed that two candidate genes (LOC11412291 and LOC11440209) may be the key genes related to grazing tolerance ofM. falcata.

Conclusion

The identified trait-associated QTLs and candidate genes in this study will provide a solid foundation for future molecular breeding for enhanced grazing-tolerance inM. falcata.
Double-seedlings and embryo-free seeds generated by genetic engineering
Authors: Xia Yumei , Wang Yao , Hu Yuanyi , Zhan Yijie , Dan Junhao , Tang Ning , Tian Junyou , Cao Mengliang
Published: October 2022

Apomixis can fix the heterosis of Hybrid F1, by maintaining its heterozygous genotype, and is an ideal way for the development of hybrid rice. In this paper, we designed an engineering strategy for realizing apomictic reproduction of hybrid rice in the way of induce adventitious embryos. An embryogenesis gene, AtWUS, controlled by the ovule-specific promoter, a ribonuclease gene Barnase driven by the egg cell-specific promoter pDD45, and an inactivation gene ZmAA1 driven by the pollen-specific promoter pG47 were simultaneously integrated into one T-DNA, and co-transformed with the second T-DNA carrying a Barstar gene. Double-seedlings were observed in transgenic line. Whole-genome sequencing and ploidy levels confirmed by flow cytometry showed that one of the double-seedlings was heterozygous diploid and the other seedling was homozygous haploid, which confirmed that embryogenesis in one of the double-seedlings arises from the zygote after fertilization and the other derived from an unfertilized gamete. Meanwhile we obtained embryo-free seeds at frequencies of 2.6% to 3.8% in T1 generation, and 0.75% to 3% in T2 generation. Though we did not obtained adventitious embryos in hybrid rice in this study, the phenomenon of double-seedlings and embryo-free seeds in transgenic line was informative and strongly suggested that endosperm development is an autonomously organized process in rice, independent of egg cell fertilization and embryo-endosperm communication. This provides novel insights into the induction of haploid embryos and lends theoretical support to successful clonal propagation using synthetic apomixis
The relationship between physical exercise and mobile phone addiction among Chinese college students: Testing mediation and moderation effects
Authors: Zeng Miaolin , Chen Siyu , Zhou Xiangyi , Zhang Jincheng , Chen Xin , Sun Jingquan
Published: October 2022

Background

During the COVID-19 pandemic, suspensions of activities and long periods of self-isolation led to a sharp increase in excessive use of mobile phones, which sparked public concern about mobile phone addiction (MPA). In recent years, more and more attention has been paid to physical exercise as a protective effect of MPA. However, more studies are needed to reveal this relationship and the exact mechanisms, based on which this study tested the mediating and moderating roles of self-control, rumination, psychological distress, and loneliness between physical exercise and MPA.

Methods

In this cross-sectional study, primary data was collected by questionnaire from 1,843 college students (19.75 ± 1.3) from five universities in Sichuan Province in Mainland China. Mobile Phone Addiction Tendency Scale (MPATS), Physical Activity Rating Scale-3 (PARS-3), Self-Control Scale (SCS), Ruminative Response Scale (RRS), Depression Anxiety Stress Scale-21 (DASS-21), and UCLA Loneliness Scale (UCLA-20) were investigated. The mediating models were examined using SPSS PROCESS macro 3.3 software, in which the mediation variables were self-control, rumination, and psychological distress, and the moderation was loneliness. Gender, major, and grade were included as control variables.

Result

Self-control, rumination, and psychological distress played a simple mediating role between physical exercise and MPA. Moreover, not only self-control and rumination but also self-control and psychological distress played the chain mediating roles between physical exercise and MPA. The chain pathways were moderated by loneliness. Specifically, the effect was more substantial among college students with higher loneliness.

Conclusion

The conclusions corroborate and clarify that self-control, rumination, and psychological distress mediated the association between physical exercise and MPA, and the mediation effects were moderated via loneliness. This present study advanced our understanding of how and when college students’ physical exercise was related to MPA. It also illustrates that educators and parents should pay more attention to college students’ physical exercise.
Assessing the relationship between terrorist attacks against ingroup or outgroup members and public support for terrorism
Authors: Schumann Sandy , Rottweiler Bettina , Gill Paul
Published: October 2022

Terrorist groups rely on constituency support for their long-term survival. Here, we examined the extent to which terrorists’ own activities are related with public opinion on terrorism. Specifically, we assessed whether more frequent and more costly terrorist attacks against the ingroup are associated with war weariness or retaliatory sentiments, thus, either weaker or stronger approval of terrorism. We further investigated if more frequent and costly attacks that target an outgroup predict higher levels of justification of terrorism. Lastly, we identified the timeframe during which domestic and outgroup terrorist attacks correlate with (lower or higher) public support. The analyses focused on Jordan (ingroup) and Israel (outgroup), over an 8-year period (2004–2011), drawing on data from the Pew Global Attitudes Survey and the Global Terrorism Database. Results showed that support for terrorism in Jordan decreased in 2005 and, again, in 2008. The frequency of terrorist attacks and fatality/injury rates in Jordan did not vary significantly during the study period. The number of attacks and fatalities/people injured in Israel, however, changed between 2004 and 2011. Cross-correlations of the time-series further demonstrated that the number of attacks and fatalities/people injured in Jordan was not related with the level of public approval of terrorism in the country. Importantly, and in line with the literature, the casualty rate in Israel was positively associated with support for terrorism in Jordan, in the next year. That is, there is evidence that more/less costly terrorist attacks on an outgroup can predict stronger/weaker public support for the tactic relatively quickly. Those findings provide insights for counter-terrorism measures.
Perceived facial happiness during conversation correlates with insular and hypothalamus activity for humans, not robots
Authors: Chaminade Thierry , Spatola Nicolas
Published: October 2022

Emotional contagion, in particular of happiness, is essential to creating social bonds. The somatic marker hypothesis posits that embodied physiological changes associated with emotions and relayed to the brain by the autonomous nervous system influence behavior. Perceiving others’ positive emotions should thus be associated with activity in brain regions relaying information from and to the autonomic nervous system. Here, we address this question using a unique corpus of brain activity recorded during unconstrained conversations between participants and a human or a humanoid robot. fMRI recordings are used to test whether activity in key brain regions of the autonomic system, the amygdala, hypothalamus, and insula, is differentially affected by the level of happiness expressed by the human and robot agents. Results indicate that for the hypothalamus and the insula, in particular the anterior agranular region strongly involved in processing social emotions, activity in the right hemisphere increases with the level of happiness expressed by the human but not the robot. Perceiving positive emotions in social interactions induces local brain responses predicted by the contagion of somatic markers of emotions only when the interacting agent is a fellow human.
Consequences of M&A team composition for deal outcomes: An inductive study
Authors: Paumen Timo , Kroon David , Khapova Svetlana N.
Published: October 2022

While Merger & Acquisition (M&A) activity has reached unprecedented levels over recent years, M&A failure rates remain high. In explaining these disappointing outcomes, previous studies barely focused on the teams that manage these M&A transactions. Furthermore, only scant information exists on team members’ roles and skill sets. With an aim to contribute to filling this gap, we inductively explore a composition logic of M&A teams and its consequences for M&A outcomes by following a grounded theory approach and conducting semi-structured interviews with 30 M&A professionals. We identify three prevailing team roles (project manager, expert and executor) which require a specific set of soft and hard skills that explain how M&A teams can enable M&A success in terms of deal execution and completion. Furthermore, we provide evidence to how aspects of project timing, such as deadlines and simultaneous projects, shape the team members’ work. Finally, our findings underline the importance of accumulating experience and learning effects on M&A deal outcomes, shaping both M&A team culture and the team members’ skill sets.
The active participation of elderly patients in traditional Chinese medicine consultations as means to creating a community of practice: A case study in Hong Kong
Authors: Pun Jack
Published: October 2022

Despite its historic role in Chinese society and its popularity with an elderly Chinese population, limited research has explored the discursive practices of Traditional Chinese Medicine (TCM). Our analysis of practitioner–patient interactions illustrated the reasons why TCM is widely welcomed by a specific patient group. This paper adopted the concept of Community of Practice (CoP) as a theoretical framework to document how patients interact with TCM practitioners to construct meaning through a process of participation and reification. This study aimed to explore how patients in a medical consultation context developed an understanding of their conditions and how they strategically used medical terminology to enact meaningful exchanges to realise their CoP. An inductive qualitative discourse analysis was conducted to for nine elderly patients (i.e., age > 65) in Hong Kong to demonstrate the role that a CoP played in a joint process of knowledge construction to promote a patient-centred practice and foster the active participation of the patients. TCM practitioners can develop specific communication skills to promote their patients’ CoP, which will improve patient-centred care, empower patients to actively engage in their treatments and result in higher levels of patient satisfaction.
High-frequency repetitive transcranial magnetic stimulation alleviates the cognitive side effects of electroconvulsive therapy in major depression
Authors: Chen Xing , Zhang Tongtong , Shan Xiaoyan , Yang Qun , Zhang Peiyun , Zhu Haijiao , Jiang Fei , Liu Chao , Li Yanzhong , Li Weijun , Xu Jian , Shen Hongmei
Published: October 2022

Objective

The retrospective study aimed to explore the difference in mood outcomes and cognitive function between high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) over dorsolateral prefrontal cortex (DLPFC) and electroconvulsive therapy in major depression disorder (MDD) patients and to examine the improvement of HF-rTMS on cognitive impairment evoked by electroconvulsive therapy (ECT).

Materials and methods

A total of 116 participants with MDD, who completed a 4-week follow-up assessment, were enrolled. The cohort consisted of 26 cases classed as control, 46 participants administrated with HF-rTMS (HF-rTMS group), 22 patients treated with ECT (ECT group), and 23 cases treated with HF-rTMS and ECT at the course of hospitalization (HF-rTMS + ECT group). Medication was kept constant as well in all participants. The 17-item Hamilton Depression Rating Scale for Depression (HAMD-17) and 14-item Hamilton Anxiety Rating Scale (HAMA-14) were used to assess depression and anxiety, respectively. Montreal Cognitive Assessment (MoCA) was to elevate cognitive function.

Results

No statistical significance was found for baseline in sociodemographic, characteristics of depression, anxiety and cognition, and psychopharmaceutic dosages among control, HF-rTMS, ECT, and HF-rTMS + ECT groups (p > 0.05). Compared with baseline level, total scores of HAMD-17 and HAMA-14 significantly decreased at the end of 4 weeks after treatment (p < 0.001). Furthermore, the decline in scores of HAMD-17 and its sleep disorder and retardation factors from baseline to post-treatment was greater in HF-rTMS, ECT, and HF-rTMS + ECT group than in control (p < 0.05), and there was a significant difference between control and HF-rTMS group in the decline of psychological factor of HAMA-14 (p < 0.01). ECT treatment evoked total score of MoCA to decrease significantly at the end of 4-week after intervention (p < 0.001), and the decline in scores of MoCA and its delayed recall and language performances from baseline to post-treatment was greater in ECT than control, HF-rTMS, and HF-rTMS + ECT (p < 0.05).

Conclusion

High-frequency repetitive transcranial magnetic stimulation improved psychological anxiety and ameliorated the cognition impairment evoked by ECT though it had the same anti-depressant efficacy as ECT.
Neural mechanism of the relationship between sleep efficiency and clinical improvement in major depressive disorder: A longitudinal functional magnetic resonance imaging study
Authors: Chen Tao , Zhao Wenming , Zhang Yu , Yu Jiakuai , Wang Ting , Zhang Jiajia , Li Yifei , Zhu Jiajia , Zhu Dao-min
Published: October 2022

Background

Antidepressants represent the most common treatment of choice for major depressive disorder (MDD). In this study, we aimed to explore the status-related changes (acute vs. remitted status) in brain function in patients with MDD.

Methods

Regular antidepressant medications (an average of 7 months after the initial visit, remitted status) were received by 48 patients with MDD. All the patients underwent MRI and polysomnography examinations as well as clinical assessment at each visit.

Results

We found that baseline fractional amplitude of low-frequency fluctuations (fALFF) of right superior parietal gyrus (SPG) and middle frontal gyrus could predict depression and anxiety symptoms improvement from acute to remitted status in patients with MDD, respectively. Moreover, we found a significant positive correlation between the fALFF of right SPG and baseline sleep efficiency (SE) in patients with MDD. Further mediation analysis revealed that the fALFF of right SPG mediated the relationship between baseline SE and depressive symptom improvement.

Conclusion

Apart from highlighting the fALFF as a potential prognostic indicator to predict and track disease progression in patients with MDD, these findings might provide a neural mechanism basis for improving sleep quality of patients with MDD and thus promoting the recovery of clinical symptoms, as well as provide a practical basis for clinical interventions in patients with MDD with sleep disorders.
Employing Respondent Driven Sampling (RDS) to recruit people who inject drugs (PWID) and other hard-to-reach populations during COVID-19: Lessons learned
Authors: Abadie Roberto , Habecker Patrick , Carrasco Kimberly Gocchi , Chiou Kathy S. , Fernando Samodha , Bennett Sydney J. , Valentin-Acevedo Aníbal , Dombrowski Kirk , West John T. , Wood Charles
Published: October 2022

Background

Respondent Driven Sampling (RDS) is an effective sampling strategy to recruit hard-to-reach populations but the impact of the COVID-19 pandemic on the use of this strategy in the collection of data involving human subjects, particularly among marginalized and vulnerable populations, is not known. Based on an ongoing study using RDS to recruit and study the interactions between HIV infection, injection drug use, and the microbiome in Puerto Rico, this paper explores the effectiveness of RDS during the pandemic and provided potential strategies that could improve recruitment and data collection.

Results

RDS was employed to evaluate its effectiveness in recruiting a group of people who inject drugs (PWID) and controls (N = 127) into a study in the midst of the COVID-19 pandemic. The participants were distributed among three subsets: 15 were HIV+ and PWID, 58 were HIV- PWID, and 54 were HIV+ and not PWID.

Findings

Results show that recruitment through peer networks using RDS was possible across all sub-groups. Yet, while those in the HIV+ PWID sub-group managed to recruit from other-sub groups of HIV- PWID and HIV+, this occurred at a lower frequency.

Conclusion

Despite the barriers introduced by COVID-19, it is clear that even in this environment, RDS continues to play a powerful role in recruiting hard-to-reach populations. Yet, more attention should be paid at how future pandemics, natural disasters, and other big events might affect RDS recruitment of vulnerable and hard-to-reach populations.
Case report: From anxiety disorders to psychosis, a continuum in transitional age youth?
Authors: Reis Joana , Marchini Simone , Nicolis Hélène , Delvenne Véronique
Published: October 2022

Introduction

To date, among individuals meeting ultra-high risk criteria for psychosis, the relationship between the presence of anxiety disorders and the risk of psychotic transition raises several unanswered questions.

Case description

This case report describes the clinical progression of a 17-year-old male initially presenting anxious symptoms meeting the DSM-V criteria for panic disorder. The patient also reported social withdraw, mild depressive symptoms, insomnia and fatigue. Over a 6 month period, a gradual onset of subthreshold psychotic symptoms suggested a prodromal phase of a psychotic disorder.

Diagnostic assessment and therapeutic intervention

A detailed assessment of UHR criteria for psychosis was performed. The overall level of social and occupational functioning was assessed by the SOFAS, which showed a 35% drop over a 12 months period. The CAARMS, has also been administered. The patient met the diagnostic criteria for UHR, APS group. The care plan included psychiatric follow-up, pharmacologic treatment, individual psychological follow-up and individual and familial psychoeducation. Over a 6 months period, the patient did not experienced a first psychotic episode and presented a partial improvement of psychotic symptoms.

Conclusion

The DSM-V categorical approach does not seem to adapt well to early clinical presentations in transitional age youth. A transdiagnostic and dimensional approach allows to better identify at-risk patients of psychiatric disorders and implement early intervention strategies.
3,4-Methylenedioxy methamphetamine, synthetic cathinones and psychedelics: From recreational to novel psychotherapeutic drugs
Authors: López-Arnau Raúl , Camarasa Jordi , Carbó Marcel·lí , Nadal-Gratacós Núria , Puigseslloses Pol , Espinosa-Velasco María , Urquizu Edurne , Escubedo Elena , Pubill David
Published: October 2022

The utility of classical drugs used to treat psychiatric disorders (e.g., antidepressants, anxiolytics) is often limited by issues of lack of efficacy, delayed onset of action or side effects. Psychoactive substances have a long history of being used as tools to alter consciousness and as a gateway to approach the unknown and the divinities. These substances were initially obtained from plants and animals and more recently by chemical synthesis, and its consumption evolved toward a more recreational use, leading to drug abuse-related disorders, trafficking, and subsequent banning by the authorities. However, these substances, by modulation of certain neurochemical pathways, have been proven to have a beneficial effect on some psychiatric disorders. This evidence obtained under medically controlled conditions and often associated with psychotherapy, makes these substances an alternative to conventional medicines, to which in many cases the patient does not respond properly. Such disorders include post-traumatic stress disease and treatment-resistant depression, for which classical drugs such as MDMA, ketamine, psilocybin and LSD, among others, have already been clinically tested, reporting successful outcomes. The irruption of new psychoactive substances (NPS), especially during the last decade and despite their recreational and illicit uses, has enlarged the library of substances with potential utility on these disorders. In fact, many of them were synthetized with therapeutic purposes and were withdrawn for concrete reasons (e.g., adverse effects, improper pharmacological profile). In this review we focus on the basis, existing evidence and possible use of synthetic cathinones and psychedelics (specially tryptamines) for the treatment of mental illnesses and the properties that should be found in NPS to obtain new therapeutic compounds.
Effect of intergenerational exchange patterns and intergenerational relationship quality on depressive symptoms in the elderly: An empirical study on CHARLS data
Authors: Zheng Ranran , Yu Mingyang , Huang Li , Wang Fang , Gao Baizhi , Fu Duanduan , Zhu Jinghui , Liu Guilin
Published: October 2022

Background

As the population ages with fewer children, depression symptoms are increasing among the elderly who lack companionship. Intergenerational support is closely related to depression in the elderly; hence how the behavioral patterns and emotional quality of intergenerational support affect depressive symptoms in the elderly should be further explored.

Objective

To study the effects of intergenerational exchange patterns and intergenerational relationship quality on depressive symptoms in the elderly.

Methods

A total of 8,015 people over 60 years old in CHARLS in 2018 were selected as the object of this study. First, the correlation between demographics, economic conditions, health status, intergenerational support patterns, intergenerational relationship quality, and depressive symptoms in the elderly were analyzed. Three regression analysis models were established to analyze the relationship between control variables, intergenerational support patterns, intergenerational relationship quality, and depressive symptoms in the elderly. Results: Among the intergenerational economic, care, and emotional exchange modes, the risk of depressive symptoms in the elderly in the mutual support group was 31.8, 38.4, and 25.5% lower than that in the non-communication group. Compared with the elderly with very poor intergenerational relationship quality, the elderly with good, very good, and excellent intergenerational relationship quality had 74.5, 84.0, and 85.6% lower risk of depressive symptoms.

Discussion

Different cultural backgrounds also affect intergenerational exchange patterns and depression in the elderly. During the study of depressive symptoms, two aspects relating to intergenerational support should be considered behaviorally and emotionally: the intergenerational exchange model and the intergenerational relationship quality. As depression in the elderly is affected by multiple factors, the participation and joint efforts of the whole society are required to reduce depressive symptoms in the elderly and realize active aging.

Conclusion

The intergenerational exchange pattern of mutual support and the higher quality of the intergenerational relationship can significantly reduce the depressive symptoms of the elderly.
Air quality assessment based on R/S–SPA composite model: A case study of the Hebei province (China)
Authors: Xing Qinfeng , Yang Ziwei , Yao Wanyan
Published: October 2022

Air pollution has been the favorite subject of pollution prevention and control measures with the aim of sustainable development. Therefore, a composite model of rescaled range (R/S) and set pair analysis (SPA) was constructed to evaluate the air pollution situation based on the data from 2013 to 2020 in the Hebei province and then predict its air quality development trend. The results show that (1) the air pollution situation is severe, but the development trend is good and (2) the main pollutants are the core factors affecting air quality, and it is necessary to focus on intervention. The innovation of this paper lies on the combination of R/S and SPA to jointly predict the development trend of air quality in the Hebei province and ensure its scientific prediction. Meanwhile, this paper does not point out the continuation cycle of development state, which is the limitation of this paper. Finally, this study deepens the understanding of air quality evaluation, and the following countermeasures are formed: adherence to the problem- and goal-oriented approach to help the transformation of low-carbon development and enhancing the awareness of energy conservation and environmental protection to practice low-carbon lifestyle.
Factors influencing negative cyber-bystander behavior: A systematic literature review
Authors: Jeyagobi Sobana , Munusamy Shalini , Kamaluddin Mohammad Rahim , Ahmad Badayai Abdul Rahman , Kumar Jaya
Published: October 2022

Cyber-aggression is global epidemic affecting citizens of cyberspace, without regards to physical, geographical and time constraints. Recent research has identified the significant role of cyber-bystanders in exacerbating and de-escalating incidents on cyber-aggression they come across. Additionally, frequent exposure to cyber-aggression is found to have been associated with negative effects on participants of cyber-aggression, ranging from self-esteem problems to mental health disorders such as depression and anxiety, and in the worst cases even suicidal behaviors and ideation. Moreover, past research had also identified that negative bystanders could potentially become aggressors themselves. Therefore, the current review is aimed at uncovering the common themes and factors that drive individuals to resort to negative bystander behavior. Hence, a systematic literature review using the PRISMA framework was carried out, involving articles published between January 2012 to March 2022, on online databases such as SCOPUS, Science Direct, SAGE Journals, Web of Science, and Springer Link. Results obtained through the synthesis of 27 selected articles, were grouped into three categories, namely situational factors, personal factors and social influence. Upon further synthesis of the results, it was noted that many of the factors had interacted with each other. Thus, practical suggestion for prevention and future research would include addressing these interactions in preventative methodologies and research interests.
Application of a person-centered prescription model improves pharmacotherapeutic indicators and reduces costs associated with pharmacological treatment in hospitalized older patients at the end of life
Authors: Ferro-Uriguen Alexander , Beobide-Telleria Idoia , Gil-Goikouria Javier , Peña-Labour Petra Teresa , Díaz-Vila Andrea , Herasme-Grullón Arlovia Teresa , Echevarría-Orella Enrique , Seco-Calvo Jesús
Published: October 2022

Objective

This study sought to investigate whether applying an adapted person-centered prescription (PCP) model reduces the total regular medications in older people admitted in a subacute hospital at the end of life (EOL), improving pharmacotherapeutic indicators and reducing the expense associated with pharmacological treatment.

Design

Randomized controlled trial. The trial was registered with ClinicalTrials.gov (NCT05454644).

Setting

A subacute hospital in Basque Country, Spain.

Subjects

Adults ≥65 years (n = 114) who were admitted to a geriatric convalescence unit and required palliative care.

Intervention

The adapted PCP model consisted of a systematic four-step process conducted by geriatricians and clinical pharmacists. Relative to the original model, this adapted model entails a protocol for the tools and assessments to be conducted on people identified as being at the EOL.

Measurements

After applying the adapted PCP model, the mean change in the number of regular drugs, STOPPFrail (Screening Tool of Older Persons' Prescriptions in Frail adults with limited life expectancy) criteria, drug burden index (DBI), drug–drug interactions, medication regimen complexity index (MRCI) and 28-days medication cost of chronic prescriptions between admission and discharge was analyzed. All patients were followed for 3 months after hospital discharge to measure the intervention's effectiveness over time on pharmacotherapeutic variables and the cost of chronic medical prescriptions.

Results

The number of regular prescribed medications at baseline was 9.0 ± 3.2 in the intervention group and 8.2 ± 3.5 in the control group. The mean change in the number of regular prescriptions at discharge was −1.74 in the intervention group and −0.07 in the control group (mean difference = 1.67 ± 0.57; p = 0.007). Applying a PCP model reduced all measured criteria compared with pre-admission (p < 0.05). At discharge, the mean change in 28-days medication cost was significantly lower in the intervention group compared with the control group (−34.91€ vs. −0.36€; p < 0.004).

Conclusion

Applying a PCP model improves pharmacotherapeutic indicators and reduces the costs associated with pharmacological treatment in hospitalized geriatric patients at the EOL, continuing for 3 months after hospital discharge. Future studies must investigate continuity in the transition between hospital care and primary care so that these new care models are offered transversally and not in isolation.
Incentives to promote accessing HIV care and viral suppression among HIV self-screening test users who obtain a reactive result
Authors: Majam Mohammad , Phatsoane Mothepane , Wonderlik Theodore , Rhagnath Naleni , Schmucker Laura K. , Singh Leanne , Rademeyer Michael , Thirumurthy Harsha , Marcus Noora , Lalla-Edward Samanta
Published: October 2022

Introduction

Achieving viral suppression in people with HIV is crucial in ending the AIDS epidemic. Among users of HIV self-screening tests, low rates of linkage to care and early retention in care are key obstacles to achieving viral suppression. This study sought to evaluate the efficacy of financial incentives in supporting HIV case management.

Methods

Young adults within the inner city of Johannesburg, South Africa and surrounding areas who used HIV self-tests, were able to use WhatsApp to communicate with study personnel, reported a reactive or invalid result, and were confirmed to by HIV-positive were enrolled in the study. Participants were randomised to an intervention arm that received reminders and financial rewards for engaging in care, or to a control arm that received the standard of care. The primary outcome was HIV viral load at six months.

Results

Among 2,388 HIV self-test kits that were distributed, 1757/2,388 (73,58%) recipients were able to use their phones to send photos to study personnel. 142/1,757 (8,08%) of these recipients reported reactive or invalid results. Upon confirmatory testing, 99/142 (69,71%) participants were identified as being HIV-positive and were enrolled in the study. 2 (1,41%) participants received an HIV negative result, and 41(28,87%) participants were either lost to follow-up or did not complete the confirmatory testing step. 20/99 (20,2%) from the intervention arm and 18/99 (18,18%) from the control arm completed the study (i.e., attended a 6 month follow up and participated in the exit interview). 29/99 (29,29%) were virally suppressed by at 6 months. Of those achieving viral suppression 15 (51,72%) were from the intervention arm.

Conclusion

Financial incentives and reminders were not effective in promoting engagement with HIV care and viral suppression in this setting.
A systematic review of the techno-economic assessment of various hydrogen production methods of power generation
Authors: Zulfhazli  , Keeley Alexander Ryota , Takeda Shutaro , Managi Shunsuke
Published: October 2022

Hydrogen is a low or zero-carbon energy source that is considered the most promising and potential energy carrier of the future. In this study, the energy sources, feedstocks, and various methods of hydrogen production from power generation are comparatively investigated in detail. In addition, this study presents an economic assessment to evaluate cost-effectiveness based on different economic indicators, including sensitivity analysis and uncertainty analysis. Proton exchange membrane fuel cell (PEMFCs) technology has the most potential to be developed compared to several other technologies. PEMFCs have been widely used in various fields and have advantages (i.e., start-up, zero-emissions, high power density). Among the various sources of uncertainty in the sensitivity analysis, the cost estimation method shows inflationary deviations from the proposed cost of capital. This is due to the selection process and untested technology. In addition, the cost of electricity and raw materials, as the main factors that are unpredictable.
Could a Medication Help With Bleeding Between the Skull and the Brain?
Authors: Chari Aswin , Lee Keng Siang , Alamri Alexander , Hutchinson Peter J. A. , Kolias Angelos G. , Edlmann Ellie
Published: October 2022

Chronic subdural haematoma is the medical name for bleeding that occurs between the skull and the brain. It usually happens to older people, like grandparents. If it causes serious symptoms, brain surgeons will operate. However, even after surgery, chronic subdural haematomas can come back, and further operations may be needed. We tested whether a medication called dexamethasone could prevent chronic subdural haematomas from coming back by performing a study of 750 people, in which half received the medication and half did not. The results showed that dexamethasone was good at stopping brain bleeds from coming back, but it had other effects that caused patients to have a more difficult recovery than did those who did not take it. These results are really important to stop doctors from giving this medication—it shows that, at the moment, surgery alone is the best treatment.
Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation
Authors: Choi Yoon , Park Su-Ryeon , Lee Sei-Jung , Choi Chang-Hyung
Published: October 2022

We present a double emulsion drop-based microfluidic approach to produce uniform polyacrylic acid functionalized polyethylene glycol (PAA-PEG) microgels. By utilizing double emulsion drops as templates, we produce monodisperse microgels by rapid photopolymerization of the inner prepolymer drop consisting of polyacrylic acid (PAA) and polyethylene glycol diacrylate (PEGDA), followed by dewetting the oil layer when they disperse into an aqueous media. The size control of the PAA-PEG microgels with a broad range is achieved by tuning the flow rate of each phase; the uniformity of the microgels is maintained even when the flow rate changes. The results show rapid R-phycoerythrin (R-PE) coupling with the microgels’ carboxylate with minimal non-specific adsorption, demonstrating highly efficient and reliable biomolecular conjugation within PAA-PEG microgels.
Modelling mechanically dominated vasculature development
Authors: Walker Benjamin J. , Dawes Adriana T.
Published: October 2022

Vascular networks play a key role in the development, function, and survival of many organisms, facilitating transport of nutrients and other critical factors within and between systems. The development of these vessel networks has been explored in a variety of in vivo, in vitro, and in silico contexts. However, the role of interactions between the growing vasculature and its environment remains largely unresolved, particularly concerning mechanical effects. Motivated by this gap in understanding, we develop a computational framework that is tailored to exploring the role of the mechanical environment on the formation of vascular networks. Here, we describe, document, implement, and explore an agent-based modelling framework, resolving the growth of individual vessels and seeking to capture phenomenology and intuitive, qualitative mechanisms. In our explorations, we demonstrate that such a model can successfully reproduce familiar network structures, whilst highlighting the roles that mechanical influences could play in vascular development. For instance, we illustrate how an external substrate could act as an effective shared memory for the periodic regrowth of vasculature. We also observe the emergence of a nuanced collective behaviour and clustered vessel growth, which results from mechanical characteristics of the external environment.
Determining the role of advection in patterning by bone morphogenetic proteins through neural network model-based acceleration of a 3D finite element model of the zebrafish embryo
Authors: Li Linlin , Wang Xu , Chai Junyi , Wang Xiaoqian , Buganza-Tepole Adrian , Umulis David M.
Published: October 2022

Embryonic development is a complex phenomenon that integrates genetic regulation and biomechanical cellular behaviors. However, the relative influence of these factors on spatiotemporal morphogen distributions is not well understood. Bone Morphogenetic Proteins (BMPs) are the primary morphogens guiding the dorsal-ventral (DV) patterning of the early zebrafish embryo, and BMP signaling is regulated by a network of extracellular and intracellular factors that impact the range and signaling of BMP ligands. Recent advances in understanding the mechanism of pattern formation support a source-sink mechanism, however, it is not clear how the source-sink mechanism shapes the morphogen patterns in three-dimensional (3D) space, nor how sensitive the pattern is to biophysical rates and boundary conditions along both the anteroposterior (AP) and DV axes of the embryo, nor how the patterns are controlled over time. Throughout blastulation and gastrulation, major cell movement, known as epiboly, happens along with the BMP-mediated DV patterning. The layer of epithelial cells begins to thin as they spread toward the vegetal pole of the embryo until it has completely engulfed the yolk cell. This dynamic domain may influence the distributions of BMP network members through advection. We developed a Finite Element Model (FEM) that incorporates all stages of zebrafish embryonic development data and solves the advection-diffusion-reaction Partial Differential Equations (PDE) in a growing domain. We use the model to investigate mechanisms in underlying BMP-driven DV patterning during epiboly. Solving the PDE is computationally expensive for parameter exploration. To overcome this obstacle, we developed a Neural Network (NN) metamodel of the 3D embryo that is accurate and fast and provided a nonlinear map between high-dimensional input and output that replaces the direct numerical simulation of the PDEs. From the modeling and acceleration by the NN metamodels, we identified the impact of advection on patterning and the influence of the dynamic expression level of regulators on the BMP signaling network.
Association of hoarding case identification and animal protection programs to socioeconomic indicators in a major metropolitan area of Brazil
Authors: de Moura Raphael Rolim , de Castro Wagner Antonio Chiba , Farinhas João Henrique , da Cunha Graziela Ribeiro , Pegoraro Martha Maria de Oliveira , Kmetiuk Louise Bach , dos Santos Andrea Pires , Biondo Alexander Welker
Published: October 2022

The present study assessed the identification of animal and object hoarding disorder cases by contact and mapping and the presence of animal protection programs in association with seven social–economic indicators of the metropolitan area of the ninth-biggest metropolitan area of Brazil. City Secretaries of Health and Environment provided demographic information and responded to a questionnaire. Overall, a very high level of hoarding case identification per municipality was associated with a higher Human Development Index, population, density, and income and related to distance from Curitiba, the capital of Parana State. Low and very low levels of hoarding case identification were related to greater area, higher Social Vulnerability Index (SVI), inequality, illiteracy, and rural areas. Very high identification level of animal protection programs was also associated with higher HDI, density and population, urban area, and high income, and geographical area. Similarly, low and very low levels of animal protection programs identification were major explained by low income, illiteracy, and distance related to higher population, urbanization, and higher HDI. In summary, better identification of hoarding cases and animal protection programs have shown an association with better socioeconomic indicators and higher population, density, and urban area. Whether municipalities with better human socioeconomic indicators may stimulate society's demands for identification of cases of individuals with hoarding disorder and animal programs should be further established. Regardless, animal health and welfare have been associated with improving human quality of life in a major Brazilian metropolitan area.
Pricing policy of organizations selling sports nutrition products in world capitals
Authors:   Russian Presidential Academy of National Economy and Public Administration, Nikolaeva M.A. , Golubtsov M.А. ,   Russian Presidential Academy of National Economy and Public Administration
Published: October 2022

The article discusses the goals, objectives and main directions of the pricing policy of organizations selling sports nutrition products in world capitals. Data on prices for the main types of sports nutrition products in 7 largest capitals of the world, including Moscow, are presented, and their comparative analysis is carried out.
Monitoring of corticobulbar motor evoked potentials during surgical remov¬al of brainstem and fourth ventricle tumors in pediatric patients
Authors:   FSAI N.N. Burdenko National Medical Research Center for Neurosurgery, Moscow, Russia, Lapteva K.N. , Ogurtsova A.A. ,   FSAI N.N. Burdenko National Medical Research Center for Neurosurgery, Moscow, Russia, Kadyrov Sh.U. ,   FSAI N.N. Burdenko National Medical Research Center for Neurosurgery, Moscow, Russia, Fomochkina L.A. ,   FSAI N.N. Burdenko National Medical Research Center for Neurosurgery, Moscow, Russia, Maryashev S.A. ,   FSAI N.N. Burdenko National Medical Research Center for Neurosurgery, Moscow, Russia
Published: October 2022

Introduction. Corticobulbar motor evoked potentials (CB-MEP) are a modality of intraoperative monitoring (IOM) for assessment of the functional state of caudal cranial nerves. CB-MEPs can be used during fossa posterior surgery, when there is a risk of damaging the caudal cranial nerves and their nuclei. The possibility of using this modality in pediatric patients is rarely discussed in the literature. Objective. To assess the CB-MEP validity in the prognosis of neurological symptoms from caudal cranial nerves in the pediatric group of patients after surgery in the brainstem and fourth ventricle. Material and methods. The study included 30 children aged from 11 months to 17 years who underwent brainstem and the fourth ventricle tumor surgery with IOM at the N.N. Burdenko National Medical Research Center for Neurosurgery from January 2020 to November 2021. Results. We obtained statistically significant difference in the dynamics of the CB-MEP amplitude in the groups of patients with and without an increase in neurological symptoms (p<0.05). Conclusion. CB-MEP can be used during brainstem surgery in pediatric patients for assessing the functional state of caudal cranial nerves.