bluuebunny/crossref_metadata_2025 huggingface dataset:
p38 MAPK in Glucose Metabolism of Skeletal Muscle: Beneficial or Harmful?
Authors: Bengal Eyal , Aviram Sharon , Hayek Tony
Published: September 2020

Skeletal muscles respond to environmental and physiological changes by varying their size, fiber type, and metabolic properties. P38 mitogen-activated protein kinase (MAPK) is one of several signaling pathways that drive the metabolic adaptation of skeletal muscle to exercise. p38 MAPK also participates in the development of pathological traits resulting from excessive caloric intake and obesity that cause metabolic syndrome and type 2 diabetes (T2D). Whereas p38 MAPK increases insulin-independent glucose uptake and oxidative metabolism in muscles during exercise, it contrastingly mediates insulin resistance and glucose intolerance during metabolic syndrome development. This article provides an overview of the apparent contradicting roles of p38 MAPK in the adaptation of skeletal muscles to exercise and to pathological conditions leading to glucose intolerance and T2D. Here, we focus on the involvement of p38 MAPK in glucose metabolism of skeletal muscle, and discuss the possibility of targeting this pathway to prevent the development of T2D.
Structure-Function Relationship of TRPM2: Recent Advances, Contradictions, and Open Questions
Authors: Kühn Frank J.P.
Published: September 2020

When in a particular scientific field, major progress is rapidly reached after a long period of relative stand-still, this is often achieved by the development or exploitation of new techniques and methods. A striking example is the new insights brought into the understanding of the gating mechanism of the transient receptor potential melastatin type 2 cation channel (TRPM2) by cryogenic electron microscopy structure analysis. When conventional methods are complemented by new ones, it is quite natural that established researchers are not fully familiar with the possibilities and limitations of the new method. On the other hand, newcomers may need some assistance in perceiving the previous knowledge in detail; they may not realize that some of their interpretations are at odds with previous results and need refinement. This may in turn trigger further studies with new and promising perspectives, combining the promises of several methodological approaches. With this review, I aim to give a comprehensive overview on functional data of several orthologous of TRPM2 that are nicely explained by structural studies. Moreover, I wish to point out some functional contradictions raised by the structural data. Finally, some open questions and some lines of possible future experimental approaches shall be discussed.
Dysmorphic Uteri: Obstetric Results after Hysteroscopic Office Metroplasty in Infertile and Recurrent Pregnancy Loss Patients. A Prospective Observational Study
Authors: Sánchez-Santiuste Mónica , Ríos Mar , Calles Laura , Cuesta Reyes de la , Engels Virginia , Pereira Augusto , Pérez-Medina Tirso
Published: September 2020

To compare the obstetric results achieved after hysteroscopic office metroplasty (HOME-DU) in infertile and recurrent pregnancy loss (RPL) patients diagnosed with dysmorphic uterus, women hysteroscopically diagnosed with dysmorphic uterus who underwent uterine-enlargement metroplasty were prospectively enrolled from June 2016 until April 2020. Patients were followed up and obstetric outcomes were recorded (pregnancy and live birth rate). Sixty-three women (30 infertile; 33 RPL) were enrolled, of which 48 became pregnant post-HOME-DU, with an overall pregnancy rate of 76.2% (66.7% among infertile participants; 84.9% among those with RPL). Overall, 64.3% (n = 36/63) achieved live birth. Among infertile women, 62.07% (n = 18/29) achieved live birth, as well as 66.7% of women with RPL (n = 18/27). The difference in live birth rates between both cohorts was 4.6% (p > 0.05). The rate of miscarriage amongst infertile patients was 3.3% (n = 1/30) and 12.1% amongst women with RPL (n = 4/33). Office metroplasty via the HOME-DU technique improves obstetric results (namely increasing live birth rate) in patients with dysmorphic uterus and a history of reproductive failure. No significant difference was found in the clinical efficacy of HOME-DU in infertile and RPL patients.
The Relevance of Dual Diagnoses among Drug-Dependent Patients with Sleep Disorders
Authors: Roncero Carlos , García-Ullán Llanyra , Bullón Alberto , Remón-Gallo Diego , Vicente-Hernández Begoña , Álvarez Ana , Caldero Amaya , Flores Andrea , Aguilar Lourdes
Published: September 2020

Background: Sleep disorders are often associated with drug use. Nearly 70% of patients admitted for detoxification report sleep problems. Dual disorder (DD) is the comorbidity between mental disorders in general and disorders related to psychoactive substance use. The association between substance use and sleep disorders (SD) appears to be bidirectional. Our objective is to analyze the association between sleep disturbance history and drug use pattern (alcohol, cannabis, opioids, and cocaine). Methods: Analysis of data in the first interview at the Addictions Unit of the Department of Psychiatry at the University of Salamanca Health Care Complex between October 2017 and January 2020. The sample consists of 398 patients. We studied the association between different variables: origin of patients (Inpatient Dual Diagnosis Detoxification Unit (IDDDU) vs. Outpatient Drug Clinic (ODC), presence of affective disorder, psychotic disorder, type of drug used, and treatment. Results: Of patients with DD, 62% had more delayed sleep induction, sleep fragmentation, early awakening, and nightmares. Outpatients had more difficulty falling asleep because, in many cases, they had not previously sought any medical assistance. On the other hand, 67% of the patients with insomnia presented depression. Conclusions: There is evidence of a harmful association between DD and SD.
An Experimental Comparison between Deep Learning and Classical Machine Learning Approaches for Writer Identification in Medieval Documents
Authors: Cilia Nicole Dalia , De Stefano Claudio , Fontanella Francesco , Marrocco Claudio , Molinara Mario , Freca Alessandra Scotto di
Published: September 2020

In the framework of palaeography, the availability of both effective image analysis algorithms, and high-quality digital images has favored the development of new applications for the study of ancient manuscripts and has provided new tools for decision-making support systems. The quality of the results provided by such applications, however, is strongly influenced by the selection of effective features, which should be able to capture the distinctive aspects to which the paleography expert is interested in. This process is very difficult to generalize due to the enormous variability in the type of ancient documents, produced in different historical periods with different languages and styles. The effect is that it is very difficult to define standard techniques that are general enough to be effectively used in any case, and this is the reason why ad-hoc systems, generally designed according to paleographers’ suggestions, have been designed for the analysis of ancient manuscripts. In recent years, there has been a growing scientific interest in the use of techniques based on deep learning (DL) for the automatic processing of ancient documents. This interest is not only due to their capability of designing high-performance pattern recognition systems, but also to their ability of automatically extracting features from raw data, without using any a priori knowledge. Moving from these considerations, the aim of this study is to verify if DL-based approaches may actually represent a general methodology for automatically designing machine learning systems for palaeography applications. To this purpose, we compared the performance of a DL-based approach with that of a “classical” machine learning one, in a particularly unfavorable case for DL, namely that of highly standardized schools. The rationale of this choice is to compare the obtainable results even when context information is present and discriminating: this information is ignored by DL approaches, while it is used by machine learning methods, making the comparison more significant. The experimental results refer to the use of a large sets of digital images extracted from an entire 12th-century Bibles, the “Avila Bible”. This manuscript, produced by several scribes who worked in different periods and in different places, represents a severe test bed to evaluate the efficiency of scribe identification systems.
Modified LOS Path Following Strategy of a Portable Modular AUV Based on Lateral Movement
Authors: Wang Xiaoming , Wu Gaosheng
Published: September 2020

The portable modular AUV (Autonomous Underwater Vehicle), named ZFAUV, has the ability to move laterally. Its turning radius becomes smaller as the forward speed decreases. Based on this special maneuverability, a modified LOS (line of sight) path following strategy, integrating basic LOS and lateral movement, is proposed. The main idea of this strategy is to improve the path following performance through cross-track error and heading error. That is to say, the ZFAUV continues to move toward the current waypoint during a survey task. If ZFAUV deviates from the desired path due to disturbances from the wind, waves, current, or other uncertainties, it gradually returns to the desired path under lateral maneuverability. At the same time, in order to reduce overshoot after reaching the current waypoint, an arc transiting strategy and decelerating strategy (if necessary) are adopted. Through this strategy, the path following performance is greatly improved. Based on mathematical modeling, this strategy is simulated with a square path and a triangular path. The same paths are selected in lake experiments. The experimental results are in agreement with the simulation results, which demonstrate the validity of this strategy.
Exploring Troubles, Attitudes, and Strategies Related to Integrated Aquaculture. A Case of the Andalusia Region (South of Spain)
Authors: Cavallo Marianna , Frangoudes Katia , Pérez Agúndez José , Raux Pascal
Published: September 2020

With the adoption of the Maritime Spatial Planning, European countries have recognized the need to move towards multi-sectoral management of marine resources and space. The present study discusses the problems and opportunities associated with an integrated aquaculture development along the coast of Andalusia through an in-depth analysis of legislative documents and face-to-face interviews. The findings suggest that the management of the aquaculture sector has been characterized by a sectoral approach with little integration into the existing economic activities and socio-cultural context leading to conflicts among fisheries and lack of acceptance from local communities. During the last decade, the regional competent authority and the aquaculture sector implemented several strategies to improve the integration with the other coastal users and enhance the social acceptability of this activity in the area.
Biomarkers for Alzheimer’s Disease Early Diagnosis
Authors: Ausó Eva , Gómez-Vicente Violeta , Esquiva Gema
Published: September 2020

Alzheimer’s disease (AD) is the most common cause of dementia, affecting the central nervous system (CNS) through the accumulation of intraneuronal neurofibrillary tau tangles (NFTs) and β-amyloid plaques. By the time AD is clinically diagnosed, neuronal loss has already occurred in many brain and retinal regions. Therefore, the availability of early and reliable diagnosis markers of the disease would allow its detection and taking preventive measures to avoid neuronal loss. Current diagnostic tools in the brain, such as magnetic resonance imaging (MRI), positron emission tomography (PET) imaging, and cerebrospinal fluid (CSF) biomarkers (Aβ and tau) detection are invasive and expensive. Brain-secreted extracellular vesicles (BEVs) isolated from peripheral blood have emerged as novel strategies in the study of AD, with enormous potential as a diagnostic evaluation of therapeutics and treatment tools. In addition; similar mechanisms of neurodegeneration have been demonstrated in the brain and the eyes of AD patients. Since the eyes are more accessible than the brain, several eye tests that detect cellular and vascular changes in the retina have also been proposed as potential screening biomarkers. The aim of this study is to summarize and discuss several potential markers in the brain, eye, blood, and other accessible biofluids like saliva and urine, and correlate them with earlier diagnosis and prognosis to identify individuals with mild symptoms prior to dementia.
Corporate Debt
Authors: Zhou Xing (Alex)
Published: September 2020

Traditional corporate bond pricing models have had limited success in explaining actual corporate yield spreads [...]
Fatty Liver, and Not Visceral Fat, Is More Associated with Liver Fibrosis and Diabetes in Non-Obese Japanese Individuals: A Cross-Sectional Study
Authors: Urata Noriyo , Kawanaka Miwa , Nishino Ken , Ishii Katsunori , Tanikawa Tomohiro , Suehiro Mitsuhiko , Sasai Takako , Haruma Ken , Kawamoto Hirofumi , Nakamura Jun , Manabe Noriaki , Kamada Tomoari
Published: September 2020

Asians are known to be more likely than Westerners to develop fatty liver and lifestyle-related diseases in spite of their weight. However, the relationship between fat accumulation and lifestyle-related diseases in non-obese Asians is unknown. Therefore, this study aimed to analyze visceral fat and hepatic fat in participants with a normal body mass index (BMI) and examine their characteristics during a medical checkup. This cross-sectional study was conducted on 663 of 1142 patients who underwent abdominal ultrasonography and who had an alcohol intake (converted to ethanol) of <30 g/day for males and <20 g/day for females and a BMI of <25 kg/m2 during a health checkup. Participants were classified into four groups: group A, visceral fat accumulation (VFA) (−) and fatty liver (FL) (−) (n = 549); group B, VFA (+) and FL (−) (n = 32); group C, VFA (−) and FL (+) (n = 58); and group D, VFA (+) and FL (+) (n = 24). The frequencies of lifestyle-related disease complications, liver function tests, and liver fibrosis were evaluated among the four groups. Compared with group A (control), groups B, C, and D had a higher number of males, BMI, abdominal circumference, ALT, AST, γ-GTP, triglyceride, uric acid, fasting blood sugar levels, and incidence of hyperlipidemia. Groups C and D had higher ALT, HbA1c, cholinesterase, and triglyceride levels, FIB4 index, and the number of patients with diabetes mellitus (DM) than groups A and B; however, there was no difference between groups A and B. FL is a risk factor of DM and liver fibrosis in non-obese Japanese individuals; however, VFA only is not a risk factor of DM and liver fibrosis.
Investigation of Thrombosis Volume, Anticoagulants, and Recurrence Factors in Portal Vein Thrombosis with Cirrhosis
Authors: Suda Tsuyoshi , Takatori Hajime , Hayashi Takehiro , Horii Rika , Nio Kouki , Terashima Takeshi , Iida Noriho , Kitahara Masaaki , Shimakami Tetsuro , Arai Kuniaki , Yamashita Taro , Yamashita Tatsuya , Mizukoshi Eishiro , Honda Masao , Okumura Kenichiro , Kozaka Kazuto , Kaneko Shuichi
Published: September 2020

This retrospective study investigated factors influencing the portal vein thrombosis (PVT) volume and recurrence in 52 cirrhosis patients with PVT from November 2008 to September 2018. All patients were treated with danaparoid sodium with or without additional antithrombin III. Blood platelet counts significantly correlated with the PVT volume (r2 = 0.17; P < 0.01). Computed tomography confirmed recurrence as PVT aggravation was reported in 43 patients, with ≥50% PVT volume reduction following anticoagulation therapy. In 43 patients, recurrence significantly correlated with the pretreatment PVT volume (P = 0.019). Factors influencing recurrence included a Child–Pugh score >8 (P = 0.049) and fibrosis index ≤7.0 based on four factors (FIB-4) (P = 0.048). Moreover, the relationship between recurrence and correlating factors showed that 15 patients who received warfarin experienced recurrence more often when Child–Pugh scores were >8 (P = 0.023), regardless of maintenance treatment. For patients who did not receive warfarin, a PVT volume ≥3.0 mL significantly influenced recurrence (P = 0.039). Therefore, the platelet count influences the PVT volume. The pretreatment PVT volume correlated with recurrence after anticoagulation therapy. According to the Kaplan–Meier curve, risk factors for PVT recurrence after anticoagulation therapy included Child–Pugh scores >8 and FIB-4 ≤7.0. Therefore, the FIB-4 is a unique factor that shows trends opposing other liver function markers.
Dental Adhesive Interfaces Reinforced with Magnetic Nanoparticles: Evaluation and Modeling with Micro-CT versus Optical Microscopy
Authors: Zaharia Cristian , Duma Virgil-Florin , Sinescu Cosmin , Socoliuc Vlad , Craciunescu Izabell , Turcu Rodica Paula , Marin Catalin Nicolae , Tudor Anca , Rominu Mihai , Negrutiu Meda-Lavinia
Published: September 2020

Dental adhesives are used in a wide range of applications, including to place direct composite restorations in frontal or posterior teeth. One of the most frequent causes for the failure of composite resin restorations is microleakages. The first aim of this work is to introduce a new type of self-etched dental adhesive doped with magnetic nanoparticles (MPs) synthetized in the laboratory. The scope is to produce adhesives with a minimized width/thickness to decrease the risk of microleakages. The second aim is to assess the width/thickness of the adhesive layer in all the characteristic areas of the teeth using both the less precise but most common optical microscopy and the more accurate and volumetric micro-Computed Tomography (CT) investigations. Twenty extracted teeth have been divided into four groups: Group 1 includes ‘blank’ samples with adhesives that are not doped with MPs; Group 2 includes samples with adhesives doped with MPs; Groups 3 and 4 include samples with adhesives doped with MPs that are subjected to an active magnetic field for 5 and 10 min, respectively. Microscopy investigations followed by micro-CT and EDAX are performed on the adhesive. While a rather good agreement is obtained between the microscopy and micro-CT results, the capability of the latter to offer a full volumetric reconstruction of the layer is exploited to analyze the adhesion of the four considered dental materials. Thus, from micro-CT results the graphs of the surface areas as functions of the adhesive layer width are modeled mathematically, as well as the volume of sealants, for each of the four groups. To our knowledge, it is the first time that such a methodology is used. Characteristic parameters are extracted and the ascertainment of the optimal parameter that should be utilized for such assessments is discussed. The study demonstrates the adhesion improvement produced for Groups 3 and 4, where MPs are used. It also concludes that the magnetic field should be applied to the adhesive material for the longest possible exposure time (with a trade-off with the clinical duration of the treatment).
Exploratory Full-Field Mechanical Analysis across the Osteochondral Tissue—Biomaterial Interface in an Ovine Model
Authors: Clark Jeffrey N. , Heyraud Agathe , Tavana Saman , Al-Jabri Talal , Tallia Francesca , Clark Brett , Blunn Gordon W. , Cobb Justin P. , Hansen Ulrich , Jones Julian R. , Jeffers Jonathan R. T.
Published: September 2020

Osteochondral injuries are increasingly prevalent, yet success in articular cartilage regeneration remains elusive, necessitating the development of new surgical interventions and novel medical devices. As part of device development, animal models are an important milestone in illustrating functionality of novel implants. Inspection of the tissue-biomaterial system is vital to understand and predict load-sharing capacity, fixation mechanics and micromotion, none of which are directly captured by traditional post-mortem techniques. This study aims to characterize the localised mechanics of an ex vivo ovine osteochondral tissue–biomaterial system extracted following six weeks in vivo testing, utilising laboratory micro-computed tomography, in situ loading and digital volume correlation. Herein, the full-field displacement and strain distributions were visualised across the interface of the system components, including newly formed tissue. The results from this exploratory study suggest that implant micromotion in respect to the surrounding tissue could be visualised in 3D across multiple loading steps. The methodology provides a non-destructive means to assess device performance holistically, informing device design to improve osteochondral regeneration strategies.
Is There a Role for Absorbable Metals in Surgery? A Systematic Review and Meta-Analysis of Mg/Mg Alloy Based Implants
Authors: Sukotjo Cortino , Lima-Neto Tiburtino J. , Santiago Júnior Joel Fereira , Faverani Leonardo P. , Miloro Michael
Published: September 2020

Magnesium (Mg) alloys have received attention in the literature as potential biomaterials for use as absorbable implants in oral and maxillofacial and orthopedic surgery applications. This study aimed to evaluate the available clinical studies related to patients who underwent bone fixation (patients), and received conventional fixation (intervention), in comparison to absorbable metals (comparison), in terms of follow-up and complications (outcomes). A systematic review and meta-analysis were performed in accordance with the PRISMA statement and PROSPERO (CRD42020188654), PICO question, ROBINS-I, and ROB scales. The relative risk (RR) of complications and failures were calculated considering a confidence interval (CI) of 95%. Eight studies (three randomized clinical trial (RCT), one retrospective studies, two case-control studies, and two prospective studies) involving 468 patients, including 230 Mg screws and 213 Titanium (Ti) screws, were analyzed. The meta-analysis did not show any significant differences when comparing the use of Mg and Ti screws for complications (p = 0.868). The estimated complication rate was 13.3% (95% CI: 8.3% to 20.6%) for the comparison group who received an absorbable Mg screw. The use of absorbable metals is feasible for clinical applications in bone surgery with equivalent outcomes to standard metal fixation devices.
Investigation of Microstructure and Mechanical Performance of IN738LC Superalloy Thin Wall Produced by Pulsed Plasma Arc Additive Manufacturing
Authors: Wang Kaibo , Sun Zhe , Liu Yuxin , Lv Yaohui
Published: September 2020

The IN738LC Ni-based superalloy strengthened by the coherent γ′-Ni3(Al,Ti) intermetallic compound is one of the most employed blade materials in gas turbine engines and IN738LC thin wall components without macro-cracks were fabricated by pulsed plasma arc additive manufacturing (PPAAM), which is more competitive when considering convenience and cost in comparison with other high-energy beam additive manufacturing technologies. The as-fabricated sample exhibited epitaxial growth columnar dendrites along the building direction with discrepant secondary arm spacing due to heat accumulation. A lot of fine γ′ particles with an average size of 81 nm and MC carbides were observed in the interdendritic region. Elemental segregation and γ–γ′ eutectic reaction were analyzed in detail and some MC carbides were confirmed in the reaction L + MC→γ + γ′. After standard heat treatment, bimodal distribution of γ′ phases, including coarse γ′ particles (385 nm, 42 vol.%) and fine γ′ particles (42 nm, 25 vol.%), was observed. The mechanism of microstructural evolution, phase formation, as well as cracking mechanisms were discussed. Microhardness and tensile tests were carried out to investigate the mechanical performance. The results show that both the as-fabricated and heat-treated samples exhibited a higher tensile strength but a slightly lower ductility compared with cast parts.
Saccharomyces cerevisiae and Candida albicans Yeast Cells Labeled with Fe(III) Complexes as MRI Probes
Authors: Patel Akanksha , Asik Didar , Snyder Eric M. , Spernyak Joseph A. , Cullen Paul J. , Morrow Janet R.
Published: September 2020

The development of MRI probes is of interest for labeling antibiotic-resistant fungal infections based on yeast. Our work showed that yeast cells can be labeled with high-spin Fe(III) complexes to produce enhanced T2 water proton relaxation. These Fe(III)-based macrocyclic complexes contained a 1,4,7-triazacyclononane framework, two pendant alcohol groups, and either a non-coordinating ancillary group and a bound water molecule or a third coordinating pendant. The Fe(III) complexes that had an open coordination site associated strongly with Saccharomyces cerevisiae upon incubation, as shown by screening using Z-spectra analysis. The incubation of one Fe(III) complex with either Saccharomyces cerevisiae or Candida albicans yeast led to an interaction with the β-glucan-based cell wall, as shown by the ready retrieval of the complex by the bidentate chelator called maltol. Other conditions, such as a heat shock treatment of the complexes, produced Fe(III) complex uptake that could not be reversed by the addition of maltol. Appending a fluorescence dye to Fe(TOB) led to uptake through secretory pathways, as shown by confocal fluorescence microscopy and by the incomplete retrieval of the Fe(III) complex by the maltol treatment. Yeast cells that were labeled with these Fe(III) complexes displayed enhanced water proton T2 relaxation, both for S. cerevisiae and for yeast and hyphal forms of C. albicans.
Spatial-Perceptual Embedding with Robust Just Noticeable Difference Model for Color Image Watermarking
Authors: Zhou Kai , Zhang Yunming , Li Jing , Zhan Yantong , Wan Wenbo
Published: September 2020

In the robust image watermarking framework, watermarks are usually embedded in the direct current (DC) coefficients in discrete cosine transform (DCT) domain, since the DC coefficients have a larger perceptual capacity than any alternating current (AC) coefficients. However, DC coefficients are also excluded from watermark embedding with the consideration of avoiding block artifacts in watermarked images. Studies on human vision suggest that perceptual characteristics can achieve better image fidelity. With this perspective, we propose a novel spatial–perceptual embedding for a color image watermarking algorithm that includes the robust just-noticeable difference (JND) guidance. The logarithmic transform function is used for quantization embedding. Meanwhile, an adaptive quantization step is modeled by incorporating the partial AC coefficients. The novelty and effectiveness of the proposed framework are supported by JND perceptual guidance for spatial pixels. Experiments validate that the proposed watermarking algorithm produces a significantly better performance.
Many Objective Optimization of a Magnetic Micro–Electro–Mechanical (MEMS) Micromirror with Bounded MP-NSGA Algorithm
Authors: Di Barba Paolo , Mognaschi Maria , Sieni Elisabetta
Published: September 2020

The paper proposes the automated optimal design of a class of micro–electro–mechanical (MEMS) devices, based on a procedure of finite element analysis coupled to evolutionary optimization algorithms. A magnetic MEMS, used as an optical switch, is considered as the case study. In particular, the geometry of the device is optimized in order to maximize the actuation torque and minimize the power losses and the device volume. The optimization algorithms belong to the genetic class and, in particular, Migrated Parents - Non-Dominated Sorting Genetic Algorithm MP-NSGA, with three objective functions, is compared to NSGA-III.
Feasibility of Automatic Seed Generation Applied to Cardiac MRI Image Analysis
Authors: Mărginean Radu , Andreica Anca , Dioşan Laura , Bálint Zoltán
Published: September 2020

We present a method of using interactive image segmentation algorithms to reduce specific image segmentation problems to the task of finding small sets of pixels identifying the regions of interest. To this end, we empirically show the feasibility of automatically generating seeds for GrowCut, a popular interactive image segmentation algorithm. The principal contribution of our paper is the proposal of a method for automating the seed generation method for the task of whole-heart segmentation of MRI scans, which achieves competitive unsupervised results (0.76 Dice on the MMWHS dataset). Moreover, we show that segmentation performance is robust to seeds with imperfect precision, suggesting that GrowCut-like algorithms can be applied to medical imaging tasks with little modeling effort.
No Resistive Normal Electrons in Beginning Superconducting States
Authors: Seo Changho , Cho Seongsoo , Koo Je Huan
Published: September 2020

We investigate why normal electrons in superconductors have no resistance. Under the same conditions, the band gap is reduced to zero as well, but normal electrons at superconducting states are condensed into this virtual energy band gap.
Energy-Efficient Clustering Routing Protocol for Wireless Sensor Networks Based on Yellow Saddle Goatfish Algorithm
Authors: Rodríguez Alma , Del-Valle-Soto Carolina , Velázquez Ramiro
Published: September 2020

The usage of wireless sensor devices in many applications, such as in the Internet of Things and monitoring in dangerous geographical spaces, has increased in recent years. However, sensor nodes have limited power, and battery replacement is not viable in most cases. Thus, energy savings in Wireless Sensor Networks (WSNs) is the primary concern in the design of efficient communication protocols. Therefore, a novel energy-efficient clustering routing protocol for WSNs based on Yellow Saddle Goatfish Algorithm (YSGA) is proposed. The protocol is intended to intensify the network lifetime by reducing energy consumption. The network considers a base station and a set of cluster heads in its cluster structure. The number of cluster heads and the selection of optimal cluster heads is determined by the YSGA algorithm, while sensor nodes are assigned to its nearest cluster head. The cluster structure of the network is reconfigured by YSGA to ensure an optimal distribution of cluster heads and reduce the transmission distance. Experiments show competitive results and demonstrate that the proposed routing protocol minimizes the energy consumption, improves the lifetime, and prolongs the stability period of the network in comparison with the stated of the art clustering routing protocols.
A Phylogenetic and Ontogenetic Perspective of the Unique Accumulation of Arterial Variations in One Human Anatomic Specimen
Authors: Pretterklieber Bettina , Pretterklieber Michael L.
Published: September 2020

Background and objectives: Anatomical dissection is an indispensable means of acquiring knowledge about the variability of the human body. We detected the co-existence of several arterial variations within one female anatomic specimen during routine anatomical dissection. The aim of this study was to evaluate if this status is a regular pattern in any of other vertebrates. Materials and Methods: Besides of a meticulous anatomic dissection, we performed a literature review concerning the frequency, the phylogenesis, and ontogenesis of all of these variations. Results: Exceptionally, the middle colic artery arose from an extraordinarily divided celiac trunk. The kidneys received three polar arteries. On the left side, a corona mortis replaced the obturator artery. The aortic arch gave rise to a bicarotid trunk, and the right subclavian artery originated and coursed as a typical lusorial artery leading to a non-recurrent laryngeal nerve on the right side. Furthermore, variations of the branches of the thyrocervical trunk were found to be present. Extraordinarily, in their cervical portion both internal carotid arteries gave rise to two arteries each. All of these variations developed within two to three weeks, around the sixth week of gestation. It was not possible to ascribe all or even one of the variations to a singular species of vertebrates. Conclusion: Apparently, arterial variations are frequently a result of random development. Medical professionals must always be aware of anatomical variations; the absence of such awareness would create major difficulties during surgery. The present case confirms the relevance of anatomical dissection, particularly for medical students.
Recurrent Superior Vena Cava Syndrome in a Patient with Sarcoidosis and Pancreatic Adenocarcinoma: A Case Report and Literature Review
Authors: Shenoy Ganesh , Kim Yunsung , Newmaster Kyra , McGillen Kathryn L. , Ruggiero Francesca , Yee Nelson S.
Published: September 2020

Background: Superior vena cava (SVC) syndrome may result from extravascular compression or intravascular obstruction such as thrombosis. Recurrent venous thrombosis is typically associated with a hypercoagulable state such as malignancy, and inheritable or acquired coagulopathy. Sarcoidosis is a derangement of the immune system, and it has been associated with malignant diseases and hypercoagulation. The association of pancreatic cancer and sarcoidosis with SVC syndrome has not been reported previously. Here, we present a case of recurrent venous thrombosis causing SVC syndrome in a patient with pancreatic ductal adenocarcinoma and underlying thoracic sarcoidosis. Methods: The patient’s electronic health record was retrospectively analyzed. Results: A 66-year-old woman with pancreatic adenocarcinoma was treated with neoadjuvant chemotherapy followed by Whipple procedure, before developing tumor recurrence in the liver. Her treatment course was complicated with repeated incidents of venous thrombosis in the presence of a central venous catheter leading to recurrent SVC syndrome, which resolved with anti-coagulation. Conclusions: This case raises a plausible inter-relationship between sarcoidosis, pancreatic cancer, and hypercoagulable state. We suggest that patients with multiple risk factors for developing venous thrombosis should be carefully monitored for any thrombotic event, and they may benefit from prophylactic anti-coagulation.
Characterization and Modeling of Room-Temperature Compressive Creep Behavior of a Near α TA31 Titanium Alloy
Authors: Yu Weixin , Hou Shusen , Yang Zhijun , Zhang Jinyong , Lang Shaoting
Published: September 2020

The creep behavior of a near α TA31 titanium alloy under different compressive pressures has been studied by long-time (up to 500 h) compression tests at room temperature. The experimental results show that several thresholds of the compressive pressure were found to exist in the compression process of the TA31 alloy. When the compressive stress is lower than 0.80Rp0.2, there is no creep. There is a steady-state creep stage at the compressive stresses between 0.85Rp0.2 and 0.93Rp0.2, in which the strain rate is approximately a constant value. When the compressive stress reaches a threshold stress between 0.93Rp0.2 and 0.95Rp0.2, the sample enters the accelerating creep stage directly. The creep model of TA31 alloy has been built by using the regression method, from which the creep strain rate of TA31 titanium alloy in the steady-state creep stage under different compressive stress levels can be calculated. The mean difference between the calculated and the experimental value is 2.54%, indicating the creep model can efficiently predict the creep behavior of TA31 alloy.
Metagenomic and Metatranscriptomic Study of Microbial Metal Resistance in an Acidic Pit Lake
Authors: Ayala-Muñoz Diana , Burgos William D. , Sánchez-España Javier , Couradeau Estelle , Falagán Carmen , Macalady Jennifer L.
Published: September 2020

Cueva de la Mora (CM) is an acidic, meromictic pit lake in the Iberian Pyrite Belt characterized by extremely high metal(loid) concentrations and strong gradients in oxygen, metal, and nutrient concentrations. We hypothesized that geochemical variations with depth would result in differences in community composition and in metal resistance strategies among active microbial populations. We also hypothesized that metal resistance gene (MRG) expression would correlate with toxicity levels for dissolved metal species in the lake. Water samples were collected in the upper oxic layer, chemocline, and deep anoxic layer of the lake for shotgun metagenomic and metatranscriptomic sequencing. Metagenomic analyses revealed dramatic differences in the composition of the microbial communities with depth, consistent with changing geochemistry. Based on relative abundance of taxa identified in each metagenome, Eukaryotes (predominantly Coccomyxa) dominated the upper layer, while Archaea (predominantly Thermoplasmatales) dominated the deep layer, and a combination of Bacteria and Eukaryotes were abundant at the chemocline. We compared metal resistance across communities using a curated list of protein-coding MRGs with KEGG Orthology identifiers (KOs) and found that there were broad differences in the metal resistance strategies (e.g., intracellular metal accumulation) expressed by Eukaryotes, Bacteria, and Archaea. Although normalized abundances of MRG and MRG expression were generally higher in the deep layer, expression of metal-specific genes was not strongly related to variations in specific metal concentrations, especially for Cu and As. We also compared MRG potential and expression in metagenome assembled genomes (MAGs) from the deep layer, where metal concentrations are highest. Consistent with previous work showing differences in metal resistance mechanisms even at the strain level, MRG expression patterns varied strongly among MAG populations from the same depth. Some MAG populations expressed very few MRG known to date, suggesting that novel metal resistance strategies remain to be discovered in uncultivated acidophiles.
New Taxa of the Family Amniculicolaceae (Pleosporales, Dothideomycetes, Ascomycota) from Freshwater Habitats in Spain
Authors: Magaña-Dueñas Viridiana , Stchigel Alberto M. , Cano-Lira José F.
Published: September 2020

With the exception of the so-called Ingoldian fungi, the diversity and distribution of the freshwater aero-aquatic or facultative fungi are not well known in Spain. In view of that, we collected and placed into wet chambers 105 samples of submerged and decomposing plant debris from various places in Spain, looking for individuals belonging to these latter two morpho-ecological groups of fungi. As a result, we found and isolated in pure culture several fungi, the morphology of some of them belonging to the family Amniculicolaceae (order Pleosporales, class Dothideomycetes). After a careful phenotypic characterization and a phylogenetic tree reconstruction using a concatenated sequence dataset of D1-D2 domains of the 28S nrRNA gene (LSU), the internal transcribed spacer region (ITS) of the nrDNA, and a fragment of the translation elongation factor 1-alpha (tef1) gene, we report the finding of three new species of the genus Murispora: Murispora navicularispora, which produces cinnamon-colored, broadly fusiform to navicular ascospores; Murispora fissilispora, which has as a remarkable characteristic the production of both sexual and asexual morphs in vitro; and Murispora asexualis, the unique species of the genus that lacks a sexual morph. As a consequence of the phylogenetic study, we introduce the new aero-aquatic genus Fouskomenomyces, with a new combination (Fouskomenomyces cupreorufescens, formerly Spirosphaera cupreorufescens as the type species of the genus) and a new species, Fouskomenomyces mimiticus; we propose the new combinations Murispora bromicola (formerly Pseudomassariosphaeria bromicola) and Murispora triseptata (formerly Pseudomassariosphaeria triseptata); and we resurrect Massariosphaeria grandispora, which is transferred to the family Lopiostomataceae.
Genomic and Transcriptome Analyses of a Thermophilic Bacterium Geobacillus stearothermophilus B5 Isolated from Compost Reveal Its Enzymatic Basis for Lignocellulose Degradation
Authors: Wang Mengmeng , Miao Jiaxi , Wang Xuanqing , Li Tuo , Zhu Han , Liu Dongyang , Shen Qirong
Published: September 2020

A lignocellulose-degrading strain isolated from thermophilic compost was identified as Geobacillus stearothermophilus B5, and found able to secrete considerable amounts of enzymes at optimal temperature (60 °C) and pH (7.5). One circular contig of 3.37 Mbp was assembled from raw data, and 3371 protein-coding genes were predicted. Clusters of orthologous groups (COG) analysis revealed various genes with functions in polymeric substrate degradation, especially for Carbohydrate Active enZymes (CAZymes), such as glycoside hydrolases (GHs) and glycosyl transferases (GTs). Furthermore, the transcriptional responses of B5 at different temperatures—with rice straw provided as the sole carbon source—were analyzed. The results revealed that B5 could resist high temperature by upregulating heat shock proteins (HSPs), enhancing protein synthesis, and decreasing carbon catabolism. Briefly, B5 possesses the ability of lignocellulose degradation, and might be considered a potential inoculant for improving composting efficiency.
Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula
Authors: Azinheiro Sarah , Carvalho Joana , Prado Marta , Garrido-Maestu Alejandro
Published: September 2020

Food poisoning continue to be a threat in the food industry showing a need to improve the detection of the pathogen responsible for the hospitalization cases and death. DNA-based techniques represent a real advantage and allow the detection of several targets at the same time, reducing cost and time of analysis. The development of new methodology using SYBR Green qPCR for the detection of L. monocytogenes, Salmonella spp. and E. coli O157 simultaneously was developed and a non-competitive internal amplification control (NC-IAC) was implemented to detect reaction inhibition. The formulation and supplementation of the enrichment medium was also optimized to allow the growth of all pathogens. The limit of detection (LoD) 95% obtained was <1 CFU/25 g for E. coli O157, and 2 CFU/25 g for Salmonella spp. and L. monocytogenes and regarding the multiplex detection a LoD 95% of 1.7 CFU/25 g was observed. The specificity, relative sensitivity and accuracy of full methodology were 100% and the use of the NC-IAC allowed the reliability of the results without interfering with the sensitivity of the methodology. The described study proved to obtain results comparable to those of probe-based qPCR, and more economically than classical high resolution melting qPCR, being both important aspects for its implementation in the food industry.
Infrared and Raman Spectroscopy of Ammoniovoltaite, (NH4)2Fe2+5Fe3+3Al(SO4)12(H2O)18
Authors: Sergeeva Anastasia V. , Zhitova Elena S. , Nuzhdaev Anton A. , Zolotarev Andrey A. , Bocharov Vladimir N. , Ismagilova Rezeda M.
Published: September 2020

Ammoniovoltaite, (NH4)2Fe2+5Fe3+3Al(SO4)12(H2O)18, is a complex hydrated sulphate of the voltaite group that has been recently discovered on the surface of the Severo-Kambalny geothermal field (Kamchatka, Russia). Vibrational spectroscopy has been applied for characterization of the mineral. Both infrared and Raman spectra of ammoniovoltaite are characterized by an abundance of bands, which corresponds to the diversity of structural fragments and variations of their local symmetry. The infrared spectrum of ammoniovoltaite is similar to that of other voltaite-related compounds. The specific feature related to the dominance of the NH4 group is its ν4 mode observed at 1432 cm−1 with a shoulder at 1510 cm−1 appearing due to NH4 disorder. The Raman spectrum of ammoniovoltaite is basically different from that of voltaite by the appearance of an intensive band centered at 3194 cm−1 and attributed to the ν3 mode of NH4. The latter can serve as a distinctive feature of ammonium in voltaite-group minerals in resemblance to recently reported results for another NH4-mineral—tschermigite, where ν3 of NH4 occurs at 3163 cm−1. The values calculated from wavenumbers of infrared bands at 3585 cm−1, 3467 cm−1 and 3400 cm−1 for hydrogen bond distances: d(O···H) and d(O···O) correspond to bonding involving H1 and H2 atoms of Fe2+X6 (X = O, OH) octahedra. The infrared bands observed at 3242 cm−1 and 2483 cm−1 are due to stronger hydrogen bonding, that may refer to non-localized H atoms of Al(H2O)6 or NH4.
First Class of Phosphorus Dendritic Compounds Containing β-Cyclodextrin Units in the Periphery Prepared by CuAAC
Authors: Sorroza-Martínez Kendra , González-Méndez Israel , Vonlanthen Mireille , Moineau-Chane Ching Kathleen I. , Caminade Anne-Marie , Illescas Javier , Rivera Ernesto
Published: September 2020

A new class of phosphorus dendritic compounds (PDCs) having a cyclotriphosphazene (P3N3) core and decorated with six β-cyclodextrin (βCD) units, named P3N3-[O-C6H4-O-(CH2)n-βCD]6, where n = 3 or 4 was designed, and the synthesis was performed using copper (I) catalyzed alkyne-azide cycloaddition (CuAAC). To obtain the complete substitution of the P3N3, two linkers consisting of an aromatic ring and an aliphatic chain of two different lengths were assessed. We found that, with both linkers, the total modification of the periphery was achieved. The two new obtained dendritic compounds presented a considerably high water solubility (>1 g/mL). The compounds comprised in this new class of PDCs are potential drug carrier candidates, since the conjugation of the βCD units to the P3N3 core through the primary face will not only serve as surface cover but, also, provide them the faculty to encapsulate various drugs inside the βCDs cavities.
Design and Synthesis of a Novel NIR Celecoxib-Based Fluorescent Probe for Cyclooxygenase-2 Targeted Bioimaging in Tumor Cells
Authors: Wang Xinli , Wang Liye , Xie Lijun , Xie Zuoxu , Li Li , Bui Dinh , Yin Taijun , Gao Song , Hu Ming
Published: September 2020

Cyclooxygenase-2 (COX-2) imaging agents are potent tools for early cancer diagnosis. Almost all of the COX2 imaging agents using celecoxib as backbone were chemically modified in the position of N-atom in the sulfonamide group. Herein, a novel COX-2 probe (CCY-5) with high targeting ability and a near-infrared wavelength (achieved by attaching a CY-5 dye on the pyrazole ring of celecoxib using a migration strategy) was evaluated for its ability to probe COX-2 in human cancer cells. CCY-5 is expected to have high binding affinity for COX-2 based on molecular docking and enzyme inhibition assay. Meanwhile, CCY-5 caused stronger fluorescence imaging of COX-2 overexpressing cancer cells (Hela and SCC-9 cells) than that of normal cell lines (RAW 264.7 cells). Lipopolysaccharide (LPS) treated RAW264.7 cells revealed an enhanced fluorescence as LPS was known to induce COX-2 in these cells. In inhibitory studies, a markedly reduced fluorescence intensity was observed in cancer cells, when they were co-treated with a COX-2 inhibitor celecoxib. Therefore, CCY-5 may be a selective bioimaging agent for cancer cells overexpressing COX-2 and could be useful as a good monitoring candidate for effective diagnosis and therapy in cancer treatment.