bluuebunny/crossref_metadata_2025 huggingface dataset:
Management of dredged marine sediments in Southern France: main keys to large-scale beneficial re-use
Authors: Dorleon Garry , Rigaud Sylvain , Techer Isabelle
Published: April 2024

Fifty million cubic meters of marine sediments are dredged each year in France in order to maintain harbor activities and sustain the economy of littoral territories. Because of anthropogenic activities in and around harbors, sediments can contain significant amounts of chemical and organic pollutants whose behavior during dredging must be addressed in order to avoid releasing risks for humans and the environment. French regulations come to govern the management of dredged sediments, considering them “safe” and possible to be dumped at sea or “contaminated” and needed to be treated on land as waste. In recent years, new constraints have been pushed toward the management of land. This management is, however, challenging as few channels are proposed to reuse marine sediments, and elimination appears to be economically and environmentally unsustainable. This study provides an overview of the technical and regulatory aspects related to dredged marine sediment management in France and aims to identify and discuss the limits of their valorization. Dredged sediments are mainly composed of particles with heterogeneous grain size, some being known for many applications such as building materials and growing media. However, several reasons have been put forward to explain why these particles are not reused when extracted from dredged sediments. Several technical, socio-economic, and regulatory obstacles explain the low demand for dredged sediments. This demand can be stimulated by government incentives and a good regulatory framework. National regulations could help streamline their reuse by removing their “waste” status and creating a regulated market for dredged sediment.
The impact of positive and negative psychological affect and overconfidence from major family events on new venture survival
Authors: Seet Pi-Shen , Tan Wee-Liang
Published: April 2024

This paper investigates how family events interacting with entrepreneurs’ psychological affect and overconfidence impact new venture viability. We use panel data from the Australian Household, Income and Labor Dynamics survey, focusing on family event-induced psychological affect entrepreneurs experience as a predictor of new venture survival. Our accelerated failure time model shows that although negative family events interact with entrepreneur overconfidence to spur cautious behaviour, positive events interacting with overconfidence have the biggest impact (negative) on new ventures. The study enhances our understanding of the embeddedness of family in the entrepreneurial process and challenges past research by revealing how positive family events can have a greater negative impact on new venture survival than negative ones.
Heart failure, recurrent vascular events and death in patients with ischemic stroke—results of the MonDAFIS study
Authors: Tütüncü Serdar , Olma Manuel C. , Kunze Claudia , Dietzel Joanna , Schurig Johannes , Rosenkranz Michael , Stingele Robert , Maschke Matthias , Heuschmann Peter U. , Kirchhof Paulus , Laufs Ulrich , Nabavi Darius G. , Röther Joachim , Thomalla Götz , Veltkamp Roland , Endres Matthias , Haeusler Karl Georg ,   for the MonDAFIS Investigators
Published: April 2024

Heart failure (HF) is associated with poor outcome after stroke, but data from large prospective trials are sparse.

We assessed the impact of HF on clinical endpoints in patients hospitalized with acute ischemic stroke or transient ischemic attack (TIA) enrolled in the prospective, multicenter Systematic Monitoring for Detection of Atrial Fibrillation in Patients with Acute Ischemic Stroke (MonDAFIS) trial. HF was defined as left ventricular ejection fraction (LVEF) < 55% or a history of HF on admission. The composite of recurrent stroke, major bleeding, myocardial infarction, and all-cause death, and its components during the subsequent 24 months were assessed. We used estimated hazard ratios in confounder-adjusted models. Overall, 410/2562 (16.0%) stroke patients fulfilled the HF criteria (i.e. 381 [14.9%] with LVEF < 55% and 29 [1.9%] based on medical history). Patients with HF had more often diabetes, coronary and peripheral arterial disease and presented with more severe strokes on admission. HF at baseline correlated with myocardial infarction (HR 2.21; 95% CI 1.02–4.79), and all-cause death (HR 1.67; 95% CI 1.12–2.50), but not with major bleed (HR 1.93; 95% CI 0.73–5.06) or recurrent stroke/TIA (HR 1.08; 95% CI 0.75–1.57). The data were adjusted for age, stroke severity, cardiovascular risk factors, and randomization. Patients with ischemic stroke or TIA and comorbid HF have a higher risk of myocardial infarction and death compared with non-HF patients whereas the risk of recurrent stroke or major hemorrhage was similar. Trial registration number Clinicaltrials.gov NCT02204267.
Holistic Processing of Sawdust to Enable Sustainable Hybrid Li-Ion Capacitors
Authors: Guo Xiaoyang , van de Kleut Dick , Zhang Jia , Chen Chaofan , Wang Xuehang , Zheng Tianye , Boles Steven
Published: April 2024

Activated carbon has long been recognized as a promising electrode material for energy storage devices. The extraordinarily high specific area makes it challenging to replace in supercapacitors since electrical double-layer capacitors need such surfaces but also porous networks to enable electrolyte penetration. As a raw material for synthesizing activated carbon, sawdust offers key benefits, such as its renewability, abundance, favorable physical attributes for energy storage, and a more environmentally friendly synthesis process compared to mined alternative sources. In this work, electrochemical characterization is carried out which highlights the critical role of pelletization in enhancing the capacitive performance of sawdust-derived activated carbon, in addition to the implicit handling and logistical benefits. Subsequently, a Li-ion capacitor is assembled with an organic solvent-based electrolyte, sawdust-derived activated carbon serving as the positive electrode, and an Al-based foil negative electrode, potentially combining high energy and power density materials into a hybrid device. Despite commendable electrochemical performance and the use of a sustainable waste-derived positive electrode with a commoditized negative electrode, challenges remain regarding the ability to mitigate the role of surface functional groups that are stabilized by bio-carbon thermal treatments. Nevertheless, this distinctive architecture holds promise as an alternative high-power energy storage technology for a future filled with renewable energy, electric vehicles, and portable electronic devices.
Pulsed Electric Field Treatment of Oat and Barley Flour: Influence on Enzymes, Non-starch Polysaccharides, Dough Rheological Properties, and Application in Flat Bread
Authors: Grgić Tomislava , Bleha Roman , Smrčkova Petra , Stulić Višnja , Pavičić Tomislava Vukušić , Synytsya Andriy , Iveković Damir , Novotni Dubravka
Published: April 2024

This study examined the effects of pulsed electric field (PEF) treatment on enzymes, non-starch polysaccharides, and bread-making potential of oat and barley flour. Enzyme activity, microstructure, β-glucan extractability, molecular weight (Mw) and structure of non-starch polysaccharides, dough rheology, and flat bread properties were determined. An exponential decay model explained better the residual activity of oat β-glucanase across electric field intensity than barley β-glucanase. PEF treatment of flour at 12 kV/cm for 162 ms significantly reduced β-glucanase activity (40.2–76.5%) while increasing the concentration of total β-glucans (33.5%) and water-extractable arabinoxylans (36–41%). Mw of linear β-d-glucans decreased (9%) while Mw of branched arabinoxylans increased (6–33%). Scanning electron microscopy showed changes in microstructure of barley proteins. Blending wheat flour (70%) with oat or barley flour (30% weight) after PEF treatment enhanced gluten aggregation energy (29–19%) and breakdown viscosity (18–43%) of dough, as well as increased β-glucan content (21–32%) but reduced specific volume (11–24%). The findings of this study provide a comprehensive insight into the PEF’s potential for retarding enzymatic reactions and preserving integrity of cereal non-starch polysaccharides.
Drug Treatment Attenuates Retinal Ganglion Cell Death by Inhibiting Collapsin Response Mediator Protein 2 Phosphorylation in Mouse Models of Normal Tension Glaucoma
Authors: Wang Yuebing , Brahma Musukha Mala , Takahashi Kazuya , Hernandez Alessandra Nolia Blanco , Ichikawa Koki , Minami Syuntaro , Goshima Yoshio , Harada Takayuki , Ohshima Toshio
Published: April 2024

Normal tension glaucoma (NTG) is a progressive neurodegenerative disease in glaucoma families. Typical glaucoma develops because of increased intraocular pressure (IOP), whereas NTG develops despite normal IOP. As a subtype of open-angle glaucoma, NTG is characterized by retinal ganglion cell (RGC) degeneration, gradual loss of axons, and injury to the optic nerve. The relationship between glutamate excitotoxicity and oxidative stress has elicited great interest in NTG studies. We recently reported that suppressing collapsin response mediator protein 2 (CRMP2) phosphorylation in S522A CRMP2 mutant (CRMP2 KIKI) mice inhibited RGC death in NTG mouse models. This study evaluated the impact of the natural compounds huperzine A (HupA) and naringenin (NAR), which have therapeutic effects against glutamate excitotoxicity and oxidative stress, on inhibiting CMRP2 phosphorylation in mice intravitreally injected with N-methyl-d-aspartate (NMDA) and GLAST mutant mice. Results of the study demonstrated that HupA and NAR significantly reduced RGC degeneration and thinning of the inner retinal layer, and inhibited the elevated CRMP2 phosphorylation. These treatments protected against glutamate excitotoxicity and suppressed oxidative stress, which could provide insight into developing new effective therapeutic strategies for NTG.
Improving the hydrostability of zeolitic imidazolate framework coatings using a facile silk fibroin protein modification method
Authors: Wei Xiuming , Chen Ting , Chen Siyu , Jia Qian , Mazlan Nurul Ain , Lewis Allana , Radacsi Norbert , Huang Yi
Published: April 2024

Zeolitic imidazolate frameworks (ZIFs) are an important subclass of metal-organic frameworks (MOFs) with zeolite-type topology, which can be fabricated under ambient synthesis conditions. However, the applications of ZIFs are commonly limited due to the weak hydrostability of their metal–ligand coordination bonds, particularly under humid and aqueous conditions. In this work, as an example, the hydrolysis behaviours of ZIF-L with a special focus on ZIF-L coatings were tested at aqueous conditions with a wide range of pHs to systematically study and fundamentally understand their structural stability and degradation mechanism. Pristine ZIF-L powder and ZIF-L coatings were severely damaged after only 24 h in aqueous media. Interestingly, the ZIF-L coatings showed two distinct hydrolyzation pathways regardless of pH conditions, exhibiting either a ring-shaped etching or unfolding behaviours. While the ZIF-L powders were hydrolyzed almost identically across all pH conditions. With this new understanding, a facile silk fibroin (SF) protein modification method was developed to enhance the hydrostability of ZIF-L coatings in aqueous media. The effect of protein concentration on surface coating was systemically studied. ZIF-L coating retained its surface morphology after soaking in water and demonstrated switchable super wetting properties and superior separation performance for oil/water mixture. As a result, the quick SF protein modification significantly enhanced the stability of ZIF-L coatings under various pHs, while retaining their switchable wetting property and excellent separation performance.
Circular RNA COL1A1 promotes Warburg effect and tumor growth in nasopharyngeal carcinoma
Authors: Zhou ZeJun , Xu Fang , Zhang Tao
Published: April 2024

Objective

Circular RNAs (circRNAs), pivotal in the pathogenesis and progression of nasopharyngeal carcinoma (NPC), remain a significant point of investigation for potential therapeutic interventions. Our research was driven by the objective to decipher the roles and underlying mechanisms of hsa_circ_0044569 (circCOL1A1) in governing the malignant phenotypes and the Warburg effect in NPC.

Methods

We systematically collected samples from NPC tissues and normal nasopharyngeal epithelial counterparts. The expression levels of circCOL1A1, microRNA-370-5p (miR-370-5p), and prothymosin alpha (PTMA) were quantitatively determined using quantitative polymerase chain reaction (qPCR) and Western blotting. Transfections in NPC cell lines were conducted using small interfering RNAs (siRNAs) or vectors carrying the pcDNA 3.1 construct for overexpression studies. We interrogated the circCOL1A1/miR-370-5p/PTMA axis's role in cellular functions through a series of assays: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide for cell viability, colony formation for growth, Transwell assays for migration and invasion, and Western blotting for protein expression profiling. To elucidate the molecular interactions, we employed luciferase reporter assays and RNA immunoprecipitation techniques.

Results

Our investigations revealed that circCOL1A1 was a stable circRNA, highly expressed in both NPC tissues and derived cell lines. A correlation analysis with clinical pathological features demonstrated a significant association between circCOL1A1 expression, lymph node metastasis, and the tumor node metastasis staging system of NPC. Functionally, silencing circCOL1A1 led to substantial suppression of cell proliferation, migration, invasion, and metabolic alterations characteristic of the Warburg effect in NPC cells. At the molecular level, circCOL1A1 appeared to modulate PTMA expression by acting as a competitive endogenous RNA or 'sponge' for miR-370-5p, which in turn promoted the malignant characteristics of NPC cells.

Conclusion

To conclude, our findings delineate that circCOL1A1 exerts its oncogenic influence in NPC through the modulation of the miR-370-5p/PTMA signaling axis.
T cell-mediated tumor killing sensitivity gene signature-based prognostic score for acute myeloid leukemia
Authors: Pan Yiyun , Xie FangFang , Zeng Wen , Chen Hailong , Chen Zhengcong , Xu Dechang , Chen Yijian
Published: April 2024

Background and Objective

Acute myeloid leukemia (AML) is an aggressive, heterogenous hematopoetic malignancies with poor long-term prognosis. T-cell mediated tumor killing plays a key role in tumor immunity. Here, we explored the prognostic performance and functional significance of a T-cell mediated tumor killing sensitivity gene (GSTTK)-based prognostic score (TTKPI).

Methods

Publicly available transcriptomic data for AML were obtained from TCGA and NCBI-GEO. GSTTK were identified from the TISIDB database. Signature GSTTK for AML were identified by differential expression analysis, COX proportional hazards and LASSO regression analysis and a comprehensive TTKPI score was constructed. Prognostic performance of the TTKPI was examined using Kaplan–Meier survival analysis, Receiver operating curves, and nomogram analysis. Association of TTKPI with clinical phenotypes, tumor immune cell infiltration patterns, checkpoint expression patterns were analysed. Drug docking was used to identify important candidate drugs based on the TTKPI-component genes.

Results

From 401 differentially expressed GSTTK in AML, 24 genes were identified as signature genes and used to construct the TTKPI score. High-TTKPI risk score predicted worse survival and good prognostic accuracy with AUC values ranging from 75 to 96%. Higher TTKPI scores were associated with older age and cancer stage, which showed improved prognostic performance when combined with TTKPI. High TTKPI was associated with lower naïve CD4 T cell and follicular helper T cell infiltrates and higher M2 macrophages/monocyte infiltration. Distinct patterns of immune checkpoint expression corresponded with TTKPI score groups. Three agents; DB11791 (Capmatinib), DB12886 (GSK-1521498) and DB14773 (Lifirafenib) were identified as candidates for AML.

Conclusion

A T-cell mediated killing sensitivity gene-based prognostic score TTKPI showed good accuracy in predicting survival in AML. TTKPI corresponded to functional and immunological features of the tumor microenvironment including checkpoint expression patterns and should be investigated for precision medicine approaches.
Convective drying of golden delicious apple enhancement: drying characteristics, artificial neural network modeling, chemical and ATR-FTIR analysis of quality parameters
Authors: TEPE Tolga Kağan
Published: April 2024

In recent years, many innovative methods have been investigated to provide alternative approaches to the food drying industry, but currently the most widely used method is convective drying. There are difficulties in integrating innovative methods into the food industry due to cost, inapplicability to every food material, or product quality, etc. In addition, it is possible to improve the convective drying method by pre-treating of foods. Thanks to the convective drying method with increased efficiency, shorter drying processes can be achieved. This study investigates the effects of ethanol and citric acid pretreatments on the convective drying process of apple slices and the drying rate, diameter and thickness shrinkage, color properties, total phenolic content (TPC), antioxidant activity (AA), ATR-FTIR spectra, and principal component analysis (PCA) of the dried samples. The results indicate that both ethanol and citric acid pretreatments significantly enhance the drying rate and decrease drying time, with the most favorable outcomes observed for apple slices immersed in an ethanol solution for 20 min. The study employs thin-layer and artificial neural network (ANN) modeling, revealing that ANN modeling outperforms thin-layer models in predicting moisture ratio. Shrinkage ratios in diameter and thickness were observed, but no significant statistical differences are found among the sample groups. The color properties of dried apple slices are influenced by pretreatments. L* values decreased in the ethanol-pretreated samples, whereas a* and b* values increased in all samples. On the other hand, drying process leads to a decrease in TPC and AA. Ethanol pretreatments caused higher losses; lower losses were observed in the citric acid–pretreated and untreated apples slices. ATR-FTIR analysis suggests distinct spectral changes in dried samples, particularly influenced by ethanol and citric acid pretreatments. The ATR-FTIR spectra highlighted shifts in water and carbohydrate levels, proteins, fibers, organic acids, and the occurrence of Maillard reactions throughout the drying process. PCA reveals that samples dried with ethanol and citric acid share a similar plane, while fresh samples and those dried at 60 °C exhibit different arrangements.
Influential lead uptake using dried and inactivated-fungal biomass obtained from Panaeolus papilionaceus: biological activity, equilibrium, and mechanism
Authors: Şenol Zeynep Mine , Keskin Zehra Saba , Dinçer Emine , Ayed Amina Ben
Published: April 2024

In this study, the use of fungal (Panaeolus papilionaceus) biomass as a biosorbent was investigated to effectively remove Pb2+ ions from aquatic medium. The removal of Pb2+ ions using a fungal biosorbent was examined in a batch system in terms of initial solution pH, temperature, time, and initial Pb2+ concentration. Optimal operating conditions for biosorption of Pb2+ ions; pH: 4.5, T: 25 °C, and t: 24 h. The max biosorption capacity for Pb2+ ions was found to be 31.2 mg g−1 from the Langmuir model. Thermodynamic studies showed that Pb2+ ions biosorption into fungal biomass was possible, spontaneous, and endothermic. Additionally, the antimicrobial activity and antibiofilm activity of the extract of fungus were also investigated. It was determined that the fungal extract did not have antimicrobial properties. On the other hand, the extract has been shown to have the potential to prevent biofilm formation. 1 mg of the extract prevented the biofilm formation of Staphylococcus aureus by 87.85%. It has been observed that the biosorption mechanism of Pb2+ ions into fungal biomass includes the steps of surface biosorption, film diffusion, and intra-particle diffusion.
Exploring mass and economic potentials of rare earth elements recycling from electric vehicles at end-of-life
Authors: Torta Gianluca , Ciacci Luca , Vassura Ivano , Passarini Fabrizio
Published: April 2024

Rare earth elements (REEs) are fundamental for various modern technologies and industrial applications. One significant application of REEs is in the production of neodymium-iron-boron (NdFeB) magnets, which are key components in electric vehicles (EVs), wind turbines, and electronic devices. These applications play a crucial role in driving the ecological and digital transition, highlighting the significance of REEs as strategic materials. With the dominance of very few countries in the REEs global supply and the rising of EVs demand, several concerns regarding resource availability, supply chain security, and price volatility have heightened the importance of efficient NdFeB magnet recycling, especially in Europe. This study assessed the elemental recycling potential of REEs from EV components through collaboration with authorized treatment facilities and metal recyclers in Italy. The study focused on three representative electric vehicles: a compact car, a van, and a hybrid vehicle. NdFeB magnets were found in various components, including the electric drive motor, air conditioning system, electric power steering, alternator, and electric gear box. The content of NdFeB magnets and REEs inside these components has been determined and economic feasibility of their recycling has been estimated by considering the intrinsic value of the raw materials contained. Despite being preliminary results, the economic value of REEs and Cu recoverable attested a promising potential for recycling, while the direct dismantling of magnets from the engine proves economically unviable for the studied components. Therefore, the study emphasizes the need for the development of specific recycling processes such as demagnetization and mechanical processing of the motors. The study also analysed the dismantling times of the target components from the vehicle and their relative economic impact on the potential for recovery.
Short carbon fiber-reinforced PLA composites: influence of 3D-printing parameters on the mechanical and structural properties
Authors: Alkabbanie Rasha , Aktas Bulent , Demircan Gokhan , Yalcin Serife
Published: April 2024

3D printing, particularly “fused filament fabrication” (FFF), plays a crucial role in Industry 4. FFF is widely used for creating complex structures and multi-material parts across various industries such as food industry, fashion industry, and manufacturing sectors. The properties of FFF-produced objects are remarkably affected by printing parameters. This study explores the impact of printing parameters and the addition of short carbon fibers on the strength of polylactic acid (PLA) printed samples. The lowering layer height, increasing feed rate and extrusion temperature boost impact strength, while a smaller raster angle enhances it. Meanwhile, an improved flexural strength is achieved by adjusting layer height, extrusion temperature, and raster angle. Higher extrusion temperatures enhance tensile strength, microstructure, and reduce porosity. Lower layer height improves flexural and impact strength (28.05% increase in 0.1 mm layer height), higher feed rate boosts strengths (12.56% improvement in 7 mm3/s feed rate), and elevated extrusion temperatures enhance impact strength (14.49% increase in 230 °C extrusion temperature) but reduce flexural strength (14.44% decrease). Incorporating carbon fibers in PLA negatively affects the microstructure but increases crystallinity, raising the melting temperature and lowering cold-crystallization temperature. The introduction of carbon fibers into PLA results in a complex interplay of mechanical and thermal properties.

Graphical abstract
Response of Carrots (Daucus carota) on the Growth, Yield, and Nutritional Composition to Varying Poultry Manure Rates
Authors: Eze Festus Onyebuchi , Mukosha Chisenga Emmanuel , Anozie Chukwunyere , Moudrý Jan , Ali Shahzaib , Ghorbani Mohammad , Amirahmadi Elnaz , Baloch Sadia Babar , Baiyeri Kayode Paul
Published: April 2024

Carrot cultivation in south-eastern Nigeria could reduce the need to transport carrots from northern regions. To explore this, a field experiment with three replications was carried out in Nsukka, south-eastern Nigeria, focusing on four carrot varieties (Carotte Touchon, Touchon Mega, Super Mega, and Touchon France) and three poultry manure rates (0, 10, and 20 t ha−1). The study revealed that Carotte Touchon had the highest root production (11.6 t ha−1) with 20 t ha−1 of manure, followed by 7.5 t ha−1 at 10 t ha−1, while Touchon Mega had the lowest root yield (1.4 t ha−1) in the control group. Proximate quality of carrots was mostly unaffected by the varieties, except for fat content. The study found significant variations in vitamins A, C, and E, phytochemicals (phytate, tannins, and oxalate), and minerals (Fe, Mg, Na, and K) among the varieties, although no impact on Zn and Ca was observed. This research suggests that carrots have substantial growth potential in south-eastern Nigeria, offering a solution to vitamin and mineral deficiencies prevalent in this area. Additionally, Carotte Touchon with either 20 t ha−1 or 10 t ha−1 of poultry manure is recommended for optimal carrot cultivation in this region.
In Situ Atomic Reconstruction Engineering Modulating Graphene-Like MXene-Based Multifunctional Electromagnetic Devices Covering Multi-Spectrum
Authors: Liu Ting-Ting , Zheng Qi , Cao Wen-Qiang , Wang Yu-Ze , Zhang Min , Zhao Quan-Liang , Cao Mao-Sheng
Published: April 2024

With the diversified development of big data, detection and precision guidance technologies, electromagnetic (EM) functional materials and devices serving multiple spectrums have become a hot topic. Exploring the multispectral response of materials is a challenging and meaningful scientific question. In this study, MXene/TiO2 hybrids with tunable conduction loss and polarization relaxation are fabricated by in situ atomic reconstruction engineering. More importantly, MXene/TiO2 hybrids exhibit adjustable spectral responses in the GHz, infrared and visible spectrums, and several EM devices are constructed based on this. An antenna array provides excellent EM energy harvesting in multiple microwave bands, with |S11| up to − 63.2 dB, and can be tuned by the degree of bending. An ultra-wideband bandpass filter realizes a passband of about 5.4 GHz and effectively suppresses the transmission of EM signals in the stopband. An infrared stealth device has an emissivity of less than 0.2 in the infrared spectrum at wavelengths of 6–14 µm. This work can provide new inspiration for the design and development of multifunctional, multi-spectrum EM devices.
Description of magnetic field lines without arcana
Authors: Escande Dominique Franck , Momo Barbara
Published: April 2024

This work is based on the variational principle for magnetic field lines introduced in 1983 by Cary and Littlejohn. The action principles for magnetic field lines and for Hamiltonian mechanics are recalled to be analogous. It is shown that the first one can be rigorously proved from first principles without analytical calculations. Not only the action principles are analogous, but also a change of canonical coordinates is recalled to be equivalent to a change of gauge. Furthermore, using the vector potential makes obvious the freedom in the choice of “time” for describing Hamiltonian dynamics. These features may be used for a new pedagogical and intuitive introduction to Hamiltonian mechanics. In the context of confined magnetic fields, the action principle for magnetic field lines makes practical calculations simpler and safer, with an intuitive background and allowing to keep a high degree of generality, as shown in the practical example of the calculation of the width of a magnetic island, analytically derived without any need of abstract Fourier components and independently of the choice of coordinates. Moreover, a new formula provides explicitly the Boozer and Hamada magnetic coordinates from action-angle coordinates.
Empirisch-kriterienorientierte Analyse des fachdidaktischen Wissens angehender Physiklehrkräfte
Authors: Zeller Jannis , Schiering Dustin , Kulgemeyer Christoph , Neumann Knut , Riese Josef , Sorge Stefan
Published: April 2024

In den letzten Jahren wurde das Professionswissen (angehender) Lehrkräfte intensiv untersucht. Neben Aussagen zur inneren Struktur liegen auch Ergebnisse über den Zusammenhang zwischen Professionswissen, Performanz in prototypischen Handlungssituationen sowie Unterrichtserfolg vor. In diesen Analysen hat sich gezeigt, dass insbesondere dem fachdidaktischen Wissen eine zentrale Rolle zukommt. Es mangelt bisher jedoch an empirisch fundierten Beschreibungen von Niveaustufen des fachdidaktischen Wissens. Zwar liegen einzelne Vorschläge vor, diese sind jedoch entweder empirisch nicht fundiert oder post hoc generiert, so dass unklar ist, inwieweit die Beschreibung der Ausprägungen auch außerhalb der jeweiligen Projektkontexte anwendbar ist. Der vorliegende Artikel stellt eine projektübergreifende Analyse des fachdidaktischen Wissens mithilfe zweier Ansätze zur Bildung von Niveaustufen vor. Dazu werden Niveaumodelle mit Daten zum fachdidaktischen Wissen aus zwei Projekten ($$N=427$$
N
=
427
und $$N=779$$
N
=
779
) mithilfe des Scale-Anchoring-Verfahrens sowie eines regressionsanalytischen Ansatzes auf Basis eines Modells hierarchischer Komplexität erstellt. Das Scale-Anchoring-Verfahren liefert Niveaubeschreibungen, die sich zwar bezüglich fachlicher und fachdidaktischer Inhalte unterschieden, aber Parallelen bezüglich lernpsychologisch interpretierbarer Operatoren zeigten. Projektübergreifend deuteten die Ergebnisse daraufhin, dass sich das fachdidaktische Wissen in niedrigen Ausprägungen auf reproduktive Aspekte beschränkt, in höheren Ausprägungen aber kreative und evaluierende Elemente hinzukommen. Das Modell hierarchischer Komplexität zeigte sich nur für einen der Datensätze als geeignet, um ein Niveaumodell abzuleiten und konnte daher für projektübergreifende Analysen nicht weiter genutzt werden. Nichtsdestotrotz lieferte die projektübergreifende Analyse mithilfe des Scale-Anchoring-Verfahrens kontextunabhängige Beschreibungen von Ausprägungen des fachdidaktischen Wissens und ermöglicht so erste Schritte in Richtung eines empirisch fundierten, inhaltlich reichhaltigen Assessments, welches über eine Einordnung mittels eines Scores hinaus geht.
Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
Authors: Panda Debasmita , Karmakar Subhasis , Dash Manaswini , Tripathy Swagat Kumar , Das Priya , Banerjee Sagar , Qi Yiping , Samantaray Sanghamitra , Mohapatra Pradipta Kumar , Baig Mirza J. , Molla Kutubuddin A.
Published: April 2024

The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage at target loci. Many target loci are inaccessible due to complex chromatin structure. Currently, there is no suitable tool available to predict the architecture of genomic target sites and their accessibility. Hence, significant time and resources are spent on performing editing experiments with inefficient guides. Although in vitro-cleavage assay could provide a rough assessment of gRNA efficiency, it largely excludes the interference of native genomic context. Transient in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic context. Here, we developed a modified protocol that offers highly efficient protoplast isolation from rice, Arabidopsis, and chickpea, using a sucrose gradient, transfection using PEG (polyethylene glycol), and validation of single guide RNAs (sgRNAs) cleavage efficiency of CRISPR-Cas9. We have optimized various parameters for PEG-mediated protoplast transfection and achieved high transfection efficiency using our protocol in both monocots and dicots. We introduced plasmid vectors containing Cas9 and sgRNAs targeting genes in rice, Arabidopsis, and chickpea protoplasts. Using dual sgRNAs, our CRISPR-deletion strategy offers straightforward detection of genome editing success by simple agarose gel electrophoresis. Sanger sequencing of PCR products confirmed the editing efficiency of specific sgRNAs. Notably, we demonstrated that isolated protoplasts can be stored for up to 24/48 h with little loss of viability, allowing a pause between isolation and transfection. This high-efficiency protocol for protoplast isolation and transfection enables rapid (less than 7 days) validation of sgRNA cleavage efficiency before proceeding with stable transformation. The isolation and transfection method can also be utilized for rapid validation of editing strategies, evaluating diverse editing reagents, regenerating plants from transfected protoplasts, gene expression studies, protein localization and functional analysis, and other applications.
Type and Location of Adenomyosis in Women with Recurrent Pregnancy Loss: A Transvaginal Ultrasonographic Assessment
Authors: Exacoustos Caterina , Ticconi Carlo , Colombi Irene , Iorio Giuseppe Gabriele , Vaquero Elena , Selntigia Aikaterini , Chiaramonte Barbara , Soreca Giorgia , Rizzo Giuseppe
Published: April 2024

The current knowledge on adenomyosis as a risk factor for RPL is very scant. Overall 120 women were included in this retrospective observational study. They were divided in three groups each of which consisted of 40 subjects: Group 1: women with RPL who were diagnosed to have adenomyosis on transvaginal ultrasound (TVS); Group 2: patients with RPL without ultrasonographic findings of adenomyosis; Group 3: patients with ultrasound diagnosis of adenomyosis without RPL and at least one live birth pregnancy. The copresence of endometriosis was also investigated. Among women with RPL, patients with adenomyosis (Group 1) had higher number of pregnancy losses (p = 0.03) and lower age at first pregnancy loss (p = 0.03) than women without adenomyosis (Group 2). Moreover, they had more frequently primary RPL (p = 0.008). Adenomyosis of the inner myometrium was found more frequently (p = 0.04) in patients of Group 1 than in patients of Group 3 in which adenomyosis was mainly in the outer myometrium (p= 0.02). No differences were found in the severity of adenomyosis between these two groups of women. TVS findings for endometriosis were observed more frequently in women with adenomyosis without RPL (Group 3) than in the other two groups of patients. Adenomyosis can be a factor involved in RPL. Differences in adenomyosis localization are associated with different risks for RPL. Patients with RPL should be investigated for the presence of adenomyosis and also for the type and localization of the disease in the different myometrial layers.
Optimizing an efficient urban tree strategy to improve microclimate conditions while considering water scarcity: a case study of Cairo
Authors: Abdelmejeed Ahmed Yasser , Gruehn Dietwald
Published: April 2024

This study aims to develop an efficient urban tree strategy (UTS) to enhance the microclimate conditions of cities that suffer from heat stress and strong solar radiation, such as the metropolitan area of Greater Cairo. Cairo recently lost its limited greenery to enhance traffic. The proposed UTS aims to achieve a balance between enhancing microclimate conditions and considering the city’s water scarcity. It seeks to consider all strategic factors suitable for local conditions, including the selection of tree species (Step 1), the utilization of new technologies for irrigation (Step 2), and the optimization of the usage of an efficient number of trees (Step 3). When applying the strategy’s recommendations to a study area within Cairo’s downtown center and when testing different tree coverage percentages within urban canyons of various aspect ratios and orientations using ENVI-met, the microclimate conditions are significantly enhanced in certain streets during summertime compared to wintertime. Applying the UTS not only enhances thermal comfort but also helps to create a better comfort zone during certain hours. In one street, for example, there are average physiological equivalent temperature (PET) reductions of − 5.18° and − 6.36° at 16:00 and 17:00, respectively, which also changes the thermal comfort zone from extreme heat stress to very heat stress. The results show a strong positive correlation between thermal comfort enhancement and a reduction in the total mean radiant temperature (TMRT), verifying that shading plays a primary role in enhancing the microclimate conditions of urban canyons. Applying the UTS to the study area significantly enhances the microclimate conditions. Furthermore, through the implementation of irrigation technologies that are part of the UTS, water demand is reduced to only 15% when trees with larger canopies are used. Additionally, when the tree coverage percentage reaches 35 to 50% in some streets, it results in a significant enhancement in the PET.
Measuring adherence to AI ethics: a methodology for assessing adherence to ethical principles in the use case of AI-enabled credit scoring application
Authors: Pokholkova Maria , Boch Auxane , Hohma Ellen , Lütge Christoph
Published: April 2024

This article discusses the critical need to find solutions for ethically assessing artificial intelligence systems, underlining the importance of ethical principles in designing, developing, and employing these systems to enhance their acceptance in society. In particular, measuring AI applications’ adherence to ethical principles is determined to be a major concern. This research proposes a methodology for measuring an application’s adherence to acknowledged ethical principles. The proposed concept is grounded in existing research on quantification, specifically, Expert Workshop, which serves as a foundation of this study. The suggested method is tested on the use case of AI-enabled Credit Scoring applications using the ethical principle of transparency as an example. AI development, AI Ethics, finance, and regulation experts were invited to a workshop. The study’s findings underscore the importance of ethical AI implementation and highlight benefits and limitations for measuring ethical adherence. A proposed methodology thus offers insights into a foundation for future AI ethics assessments within and outside the financial industry, promoting responsible AI practices and constructive dialogue.
Crossing the principle–practice gap in AI ethics with ethical problem-solving
Authors: Corrêa Nicholas Kluge , Santos James William , Galvão Camila , Pasetti Marcelo , Schiavon Dieine , Naqvi Faizah , Hossain Robayet , Oliveira Nythamar De
Published: April 2024

The past years have presented a surge in (AI) development, fueled by breakthroughs in deep learning, increased computational power, and substantial investments in the field. Given the generative capabilities of more recent AI systems, the era of large-scale AI models has transformed various domains that intersect our daily lives. However, this progress raises concerns about the balance between technological advancement, ethical considerations, safety measures, and financial interests. Moreover, using such systems in sensitive areas amplifies our general ethical awareness, prompting a re-emergence of debates on governance, regulation, and human values. However, amidst this landscape, how to bridge the principle–practice gap separating ethical discourse from the technical side of AI development remains an open problem. In response to this challenge, the present work proposes a framework to help shorten this gap: ethical problem-solving (EPS). EPS is a methodology promoting responsible, human-centric, and value-oriented AI development. The framework’s core resides in translating principles into practical implementations using impact assessment surveys and a differential recommendation methodology. We utilize EPS as a blueprint to propose the implementation of an Ethics as a Service Platform, currently available as a simple demonstration. We released all framework components openly and with a permissive license, hoping the community would adopt and extend our efforts into other contexts. Available in the following URL https://nkluge-correa.github.io/ethical-problem-solving/.
A study about the prediction of population growth and demographic transition in Bangladesh
Authors: Karim Rezaul , Pk M. A. Bkar , Dey Pinakee , Akbar M. Ali , Osman M. S.
Published: April 2024

Bangladesh is the smallest nation in South Asia, with the eighth-largest and tenth-largest population density globally. The main goal of this article is to find the population growth and demographic transition (DT) in Bangladesh. The time series data used in this paper were gathered from population surveys, national censuses, and UN Population division, population projections and estimates. There are numerous variations in the population's size and age distribution due to Bangladesh's continuing demographic transition, which creates possibilities for the economy and society and difficulties for policy. It must be successfully controlled to develop this population into stronger and more stable economic and social development. Bangladesh's population and policymakers observe increasing population growth concerns, as the country's economy and policies heavily depend on both the population's size and growth rate. Bangladesh's changing demographics are creating new economic opportunities, and the findings presented in this paper could be useful to planners and policymakers to enhance Bangladesh's population policy. At the end of the pioneering study, we presented graphically what the population of Bangladesh could be in 2000- 2100 and the impact it would have on Bangladesh.
Mitochondrial homeostasis: shaping health and disease
Authors: Li Xiaoyu , Jiang Ou , Chen Mo , Wang Songlin
Published: April 2024

Mitochondria serve as the primary site for metabolizing the three major nutrients, underscoring their pivotal role in cellular energy metabolism and the regulation of signaling pathways. Mitochondrial homeostatic imbalance is a key pathological cause of the development of many diseases. Hence, preserving mitochondrial homeostasis is vital for the normal growth and development of cells and organisms. Living organisms have evolved intricate regulatory mechanisms to ensure cellular mitochondrial homeostasis. This review focuses on recent advancements in comprehending the mechanisms responsible for maintaining mitochondrial homeostasis and addresses the current challenges in this field. We also provide an overview of the key functions of mitochondria in both physiological and pathological conditions. Emphasizing the potential therapeutic implications, we discuss strategies for preserving mitochondrial homeostasis, recognizing its significance in mitigating various health conditions.

Graphical Abstract
A Flexible Big Data System for Credibility-Based Filtering of Social Media Information According to Expertise
Authors: Diaz-Garcia Jose A. , Gutiérrez-Batista Karel , Fernandez-Basso Carlos , Ruiz M. Dolores , Martin-Bautista Maria J.
Published: April 2024

Nowadays, social networks have taken on an irreplaceable role as sources of information. Millions of people use them daily to find out about the issues of the moment. This success has meant that the amount of content present in social networks is unmanageable and, in many cases, fake or non-credible. Therefore, a correct pre-processing of the data is necessary if we want to obtain knowledge and value from these data sets. In this paper, we propose a new data pre-processing technique based on Big Data that seeks to solve two of the key concepts of the Big Data paradigm, data validity and credibility of the data and volume. The system is a Spark-based filter that allows us to flexibly select credible users related to a given topic under analysis, reducing the volume of data and keeping only valid data for the problem under study. The proposed system uses the power of word embeddings in conjunction with other text mining and natural language processing techniques. The system has been validated using three real-world use cases.
Antenatal Depression and its Associated Factors: Findings from Kuwait Birth Cohort Study
Authors: Al-Sabah Reem , Al-Taiar Abdullah , Ziyab Ali H. , Akhtar Saeed , Hammoud Majeda S.
Published: April 2024

Background

Pregnant and postpartum women are at high risk of depression due to hormonal and biological changes. Antenatal depression is understudied compared to postpartum depression and its predictors remain highly controversial.

Aim

To estimate the prevalence of depressive symptoms during pregnancy and investigate factors associated with this condition including vitamin D, folate and Vitamin B12 among participants in the Kuwait Birth Study.

Methods

Data collection occurred as part of the Kuwait Birth Cohort Study in which pregnant women were recruited in the second and third trimester during antenatal care visits. Data on antenatal depression were collected using the Edinburgh Postnatal Depression Scale (EPDS), considering a score of ≥ 13 as an indicator of depression. Logistic regression was used to investigate factors associated with depressive symptoms in pregnant women.

Results

Of 1108 participants in the Kuwait Birth Cohort study, 1070(96.6%) completed the EPDS. The prevalence of depressive symptoms was 21.03%(95%CI:18.62–23.59%) and 17.85%(95%CI:15.60-20.28%) as indicated by an EPDS ≥ 13 and EPDS ≥ 14 respectively. In the multivariable analysis, passive smoking at home, experiencing stressful life events during pregnancy, and a lower level of vitamin B12 were identified as predisposing factors. Conversely, having desire for the pregnancy and consumption of fruits and vegetables were inversely associated with depressive symptoms.

Conclusion

Approximately, one fifth of pregnant women had depressive symptoms indicating the need to implement screening program for depression in pregnant women, a measure not systematically implemented in Kuwait. Specifically, screening efforts should focus on pregnant women with unintended pregnancies, exposure to passive smoking at home, and recent stressful live events.