bluuebunny/crossref_metadata_2025 huggingface dataset:
Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS
Authors: Russo Francesco , Chimienti Guglielmina , Riezzo Giuseppe , Linsalata Michele , D’Attoma Benedetta , Clemente Caterina , Orlando Antonella
Published: November 2018

Background. Alterations of the small-intestinal permeability (s-IP) might play an essential role in a subgroup of diarrhoea-predominant IBS (D-IBS) patients. Goals. (a) To analyse in D-IBS patients the symptom profile in relation to the altered (+) or not (−) s-IP using the Gastrointestinal Symptom Rating Scale (GSRS). (b) To assess the circulating levels of the adipokines IL-6, IL-8, TNF-α, leptin, and adiponectin, along with LPS, TLR-4, neurotensin, and brain-derived neurotrophic factor (BDNF). The frequency distribution of SNPs at the loci for the investigated molecules and leptin receptor was evaluated. Study. The study included 34 D-IBS patients and 17 healthy controls (HC). s-IP permeability was assayed by high-performance liquid chromatography determination in the urine of the lactulose to mannitol ratio. Concentrations of IL-6, IL-8, TNF-α, LPS, TLR-4, leptin, adiponectin, neurotensin, and BDNF were assayed by ELISA. Screening of genetic variants was done employing the restriction fragment length polymorphism-polymerase chain reaction method. Results. D-IBS(−) patients had a significantly higher GSRS cluster pain and diarrhoea profile than D-IBS(+) ones. Significant correlations were found between the symptoms clusters and immune activation and inflammation markers. The levels of adipo(cyto)kines in D-IBS(+) patients were higher than those of controls, and IL-6 levels correlated with those of LPS. Leptin and BDNF were significantly higher, and neurotensin levels were significantly lower in D-IBS(+) than in controls. No differences were found in the frequency distribution of genotypes among the study groups. Conclusions. Results from this study could be of some help in the characterization of the D-IBS and highlight the contribution of an altered intestinal barrier in the pathogenesis of this syndrome. Besides, a role could be ascribed to molecules secreted by the visceral adipose tissue that can impact on barrier functions.
Low Serum IGF-1 in Boys with Recent Onset of Juvenile Idiopathic Arthritis
Authors: Lundell Anna-Carin , Erlandsson Malin , Bokarewa Maria , Liivamägi Hille , Uibo Karin , Tarraste Sirje , Rebane Tiina , Talvik Tiina , Pruunsild Chris , Pullerits Rille
Published: November 2018

Background. Liver-derived insulin-like growth factor-1 (IGF-1) contributes bone formation. Decreased IGF-1 levels are common in juvenile idiopathic arthritis (JIA), but whether IGF-1 is related to sex and differ during the pathogenic progress of JIA is unknown. Objective. The aim of this study was to examine IGF-1 levels in boys and girls with newly diagnosed JIA, with established JIA and in controls. Methods. The study group included 131 patients from the Estonian population-based prevalence JIA study. Blood samples were obtained from 27 boys and 38 girls with early JIA (≤1 month from the diagnosis), 29 boys and 36 girls with established JIA (mean disease duration 18 months), and from 47 age- and sex-matched controls. Results. IGF-1 levels in boys were significantly decreased in early JIA compared to male controls, while IGF-1 levels in girls were comparable between JIA and controls. In early JIA, IGF-1 levels were 12-fold lower in boys relative to girls. In controls, IGF-1 levels correlated with both age and height, while these correlations were lost in boys with early JIA. Conclusion. We report a sex-dependent deficiency in serum IGF-1 in boys with early JIA, which argues for sex-related differences in biological mechanisms involved in the disease pathogenesis.
Massive Leiomyomatous Uterine Proliferation Following Kidney Transplantation: A Case Report and Literature Review
Authors: Minja Emmanuel J. , Tan Miguel , Gibbs Melissa J. , Kazimi Marwan M. , Hundley Jonathan C. , Pollinger Harrison S.
Published: November 2018

Uterine fibroids are the most common benign uterine tumors affecting > 50% of premenopausal women. The incidence, burden and symptoms from uterine fibroids are higher in women of African descent compared to Caucasians. Despite increasing number of African American females being evaluated for and undergoing kidney transplantation (KT), perioperative management guidelines for uterine fibroids currently do not exist. We present a case of a 40 y/o African American female with known symptomatic uterine fibroids preoperatively and medically managed, who underwent a successful KT and 4 years later progressively developed massive leiomyomatous uterine proliferation, causing a complete lateral displacement of the transplanted kidney with severe hydronephrosis, transplant ureteral obstruction and secondary urinary tract infections with bacteremia. This obstruction required a percutaneous nephrostomy tube placement followed by an interval transabdominal hysterectomy, which was complicated by transplant ureteral transection requiring ureteral reimplantation, resulting in prolonged hospitalization, follow-up and outpatient antibiotic regimen. There is a need for management guidelines for uterine fibroids incidentally encountered during the KT evaluation process to avoid similar preventable post-KT complications in patient populations most commonly affected. Literature review and perioperative management/surveillance strategies are provided.
NRF2 through RPS6 Activation Is Related to Anti-HER2 Drug Resistance in HER2-Amplified Gastric Cancer
Authors: Gambardella Valentina , Gimeno-Valiente Francisco , Tarazona Noelia , Ciarpaglini Carolina Martinez , Roda Desamparados , Fleitas Tania , Tolosa Pablo , Cejalvo Juan Miguel , Huerta Marisol , Roselló Susana , Castillo Josefa , Cervantes Andrés
Published: November 2018

Purpose:

Despite the clinical advantage of the combination of trastuzumab and platinum-based chemotherapy in HER2-amplified tumors, resistance will eventually develop. The identification of molecular mechanisms related to primary and acquired resistance is needed.

Experimental Design:

We generated lapatinib- and trastuzumab-resistant clones deriving from two different HER2-amplified gastric cancer cell lines. Molecular changes such as protein expression and gene-expression profile were evaluated to detect alterations that could be related to resistance. Functional studies in vitro were corroborated in vivo. The translational relevance of our findings was verified in a patient cohort.

Results:

We found RPS6 activation and NRF2 to be related to anti-HER2 drug resistance. RPS6 or NRF2 inhibition with siRNA reduced viability and resistance to anti-HER2 drugs. In knockdown cells for RPS6, a decrease of NRF2 expression was demonstrated, suggesting a potential link between these two proteins. The use of a PI3K/TORC1/TORC2 inhibitor, tested in vitro and in vivo, inhibited pRPS6 and NRF2 expression and caused cell and tumor growth reduction, in anti–HER2-resistant models. In a cohort of HER2-amplified patients treated with trastuzumab and chemotherapy, a high level of NRF2 at baseline corresponds with worse progression-free survival.

Conclusions:

NRF2 through the PI3K/AKT/mTOR/RPS6 pathway could be a potential effector of resistance to anti-HER2 drugs in our models. RPS6 inhibition decreases NRF2 expression and restores sensitivity in HER2-amplified gastric cancer in vitro and in vivo. High NRF2 expression in gastric cancer patients predicts resistance to treatment. RPS6 and NRF2 inhibition could prevent resistance to anti-HER2 drugs.
The Present Status and Future of Barrett’s Esophageal Adenocarcinoma in Japan
Authors: Nishi Takayuki , Makuuchi Hiroyasu , Ozawa Soji , Shimada Hideo , Chino Osamu
Published: November 2018

Background: The incidence of esophageal adenocarcinoma in Europe and the United States rapidly increased from the latter half of the 1970s and exceeded that of esophageal squamous cell carcinoma in the latter half of the 1990s, currently accounting for approximately 60% of all esophageal carcinomas. Recently, its incidence has also increased in Japan, raising concerns that it will follow a course similar to that in Europe and the United States. Summary: The incidence of esophageal adenocarcinoma in Japan was about 2% until the 1990s, but in recent years, it has risen to 6.5–7.1%. Causes include the increase in the incidence of obesity due to changes in eating habits with resultant increases in the incidence of hiatal hernia and reflux esophagitis, a decrease in the rate of Helicobacter pylori infection, and the increased interest of physicians in the gastroesophageal junction. The number of gastroesophageal reflux disease patients in Japan rapidly increased from the 1990s, which accordingly increased the number of Barrett’s esophageal adenocarcinoma patients from the latter half of the 1990s. Tabulation and analysis of 1,794 reported cases of Barrett’s esophageal adenocarcinoma in Japan showed that superficial cancers accounted for 77.6%, and that the concomitant rates of hiatal hernia and reflux esophagitis were high at 87 and 70% respectively. Key Message: The future trend in the incidence of Barrett’s esophageal adenocarcinoma in Japan will depend on the increase in the incidence of reflux esophagitis, which is essential for the development of Barrett’s esophagus and Barrett’s esophageal carcinoma. The obesity rate is lower in Japan than that in Europe and the United States, and the incidence and severity of reflux esophagitis are low. We expect that the incidence of Barrett’s esophageal adenocarcinoma in Japan will not rise as high as in Europe and the United States, and will remain below 10%.
The Difficult Secondary Tracheoesophageal Puncture: A Technique for Safe Insertion
Authors: Abdul-Aziz Dunia , Zenga Joseph , Deschler Daniel G.
Published: November 2018

Background/Aims: The tracheoesophageal prothesis (TEP) has become the primary modality for laryngeal communication after total laryngectomy due to high success rates, minimal morbidity, and more natural pulmonary driven speech. Fibrosis, kyphosis, and post-radiation contracture may preclude TEP placement through rigid esophagoscopy, and certain patients may not tolerate an in-office awake procedure. For such patients, a technique for flexible esophageal stenting and TEP placement is necessary. Methods: We performed a retrospective review of 3 patients who underwent TEP placement through endotracheal-tube esophageal stenting at the Massachusetts Eye and Ear Infirmary. Results: All 3 patients underwent laryngectomy after prior chemoradiotherapy for laryngeal cancer with resulting neck contracture and fibrosis preventing rigid esophagoscopy. All patients underwent successful TEP placement through endotracheal stenting without complication and developed excellent tracheoesophageal speech. Specific technical details are highlighted. Conclusions: In patients with anatomical constraints preventing traditional TEP placement through rigid esophagoscopy, fiberoptic guidance through an endotracheal tube stent provides a safe and efficient approach for TEP placement.
Severe Diarrhea in the Setting of Immune Checkpoint Inhibitors
Authors: Kuo Jean R. , Davis Amy D. , Rodriguez Eduardo A. , Vela Marcelo F. , Heigh Russell I. , Salomao Marcela A. , Gurudu Suryakanth R.
Published: November 2018

Immune checkpoint inhibitors (ICPIs) are novel therapeutic agents targeting a variety of cancers by enhanced T cell activation. Immune-related adverse events (irAEs) commonly occur with ICPI use and can affect multiple organ systems including the gastrointestinal tract. Due to irAEs, the use of ICPIs is limited in autoimmune diseases. We present a case of microscopic colitis diagnosed after the initiation of nivolumab and a case of ipilimumab colitis and Clostridium difficile in the setting of Crohn’s colitis.
The Impact of Late Onset Arterial Hypotension on Respiratory Outcome in Extremely Premature Infants
Authors: Kurtom Waleed , Jain Deepak , Quan Meiying , Vanbuskirk Silvia , Bancalari Eduardo , Claure Nelson
Published: November 2018

Background: In extremely premature infants, arterial hypotension in the first days after birth has been associated with an increased risk for bronchopulmonary dysplasia (BPD). Some infants present with hypotension at a later postnatal age, but the relationship between late onset hypotension (LOH) and BPD has not been evaluated. Objective: To evaluate the association between LOH and BPD and to identify pre- and postnatal factors associated with LOH. Methods: Prospectively collected data from a cohort of 23–28 weeks gestational age (GA) infants born during years 2005–2015 and alive at day 28 were analyzed. LOH was defined as the receipt of vasopressor treatment during days 8–28. BPD was defined as need for oxygen at 36 weeks postmenstrual age. Late mortality was defined as death after day 28. Results: Of 1,058 infants in the cohort, 90 (9%) had LOH during days 8–28. Infants with LOH had a higher incidence of BPD than normotensive infants (55 vs. 21%, p < 0.001). Multivariate logistic regression analysis (LRA) showed that LOH was associated with an increased risk for BPD (OR 1.87, 95% CI 1.10–3.17). LOH was also associated with an increased risk for late mortality. LRA showed the risk for LOH increased with lower GA, sepsis and patent ductus arteriosus during days 8–28. Conclusions: In this cohort of extremely premature infants, LOH was associated with an increased the risk for BPD. This association could be secondary to underlying factors that predispose to LOH and BPD or to the deleterious effects of LOH or its treatments on the lung. Further investigation is needed to assess causality.
Vascular Remodeling Process of Heparin-Conjugated Poly(ε-Caprolactone) Scaffold in a Rat Abdominal Aorta Replacement Model
Authors: Xu Zeqin , Gu Yongquan , Li Jianxin , Feng Zengguo , Guo Lianrui , Tong Zhu , Ye Lin , Wang Chunmei , Wang Rong , Geng Xue , Wang Cong , Zhang Jian
Published: November 2018

In the field of vascular graft research, poly-ε-caprolactone (PCL) is used owing to its good mechanical strength and biocompatibility. In this study, PCL scaffold was prepared by electrospinning and surface modification with heparin via hexamethylenediamine. Then the scaffolds were implanted into the infrarenal abdominal aorta of Wistar rats and contrast-enhanced micro-ultrasound was used to monitor the patency of grafts after implantation. These grafts were extracted from the rats at 1, 3, and 6 months for histological analysis, immunofluorescence staining, and scanning electron microscopy observation. Although some grafts experienced aneurysmal change, results showed that all implanted grafts were patent during the course of 6 months and these grafts demonstrated well-organized neotissue with endothelium formation, smooth muscle regeneration, and extracellular matrix formation. Such findings confirm feasibility to create heparin-conjugated scaffolds of next-generation vascular grafts.
Endovascular Treatment of a Dural Arteriovenous Fistula after Cerebral Sinovenous Thrombosis in a Child
Authors: Gatto Luana Antunes Maranha , De Souza Thiago Ferreira Simões  , Naves de Lima Alves Guilherme , Padilha Miranda Tayna , Koppe Gelson Luis , Demartini, Jr. Zeferino
Published: November 2018

Background: Dural arteriovenous fistula (DAVF) is rare in children. Development theories postulate a response to cerebral sinovenous thrombosis (CSVT) or to venous hypertension. The symptoms are highly nonspecific and depend on lesion location. Standard treatment of thrombosis is based on antithrombotic therapy, while the main therapy for DAVF is embolization. Case Report: An 8-year-old boy presented with headache was diagnosed with CSVT and treated with anticoagulant. He developed tinnitus, mental confusion, and lowering of consciousness. Magnetic resonance imaging showed a DAVF draining through a single stenotic venous sinus. Successful endovascular treatment was performed with arterial embolization of multiple feeders and stent and balloon sinus angioplasty. Conclusion: Risk factors associated with sinus thrombosis must be always investigated; endovascular treatment is safe and reasonable in a clinical deterioration scenario.
Delayed Breast Cellulitis following Surgery for Breast Cancer: A Literature Review
Authors: Exarchos Georgios , Metaxa Linda , Constantinidou Anastasia , Kontos Michalis
Published: November 2018

Background: Delayed breast cellulitis (DBC) is a relatively rare complication following breast-conserving surgery. It is often challenging to distinguish DBC from other clinical conditions such as postoperative infection, inflammatory reaction following radiation, and recurrent inflammatory carcinoma. The definition of DBC, diagnostic approach, and treatment are not well established in the literature. Methods: We performed a literature search with the keywords ‘Delayed breast cellulitis' and ‘Breast conservation therapy cellulitis', without limitations to the dates or the article types, in the PubMed database. Information about the number of cases with DBC, the age of the patients, the interval between the onset of symptoms and the time of surgery or radiotherapy, and the type and outcome of DBC treatment were reviewed and tabulated. Results: We identified only 5 papers that were absolutely related to our subject, reflecting the fact that ‘delayed breast cellulitis' is a fairly unknown term and the condition is rather underreported. Although most agree that DBC is primarily an aseptic inflammatory process, bacterial growth may contribute to its development or recurrence. Obesity, breast size, location of the breast tumor, removal of the axillary lymph nodes, and connective tissue disorders are considered as risk factors. There is no clear evidence on how DBC should be best managed. Antibiotic treatment is controversial, and many authors suggest anti-inflammatory agents or sole observation. Prevention of lymph stasis and its consequences with massage and skin care may be helpful. Despite the fact that malignancy is rare, in cases where the condition persists for more than 4 months, a core biopsy should be performed to rule out recurrent or second primary carcinoma. Conclusion: The correct diagnostic approach is essential as it provides patients with reassurance, minimizes anxiety, and prevents unnecessary medical investigations, treatments, and costs.
Identification of Candidate Genes for Mayer-Rokitansky-Küster-Hauser Syndrome Using Genomic Approaches
Authors: Backhouse Brendan , Hanna Chloe , Robevska Gorjana , van den Bergen Jocelyn , Pelosi Emanuele , Simons Cas , Koopman Peter , Juniarto A. Zulfa , Grover Sonia , Faradz Sultana , Sinclair Andrew , Ayers Katie , Tan Tiong Y.
Published: November 2018

Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is a disorder of sex development which affects 1 in 4,500 females and is characterized by agenesis of müllerian structures, including the uterus, cervix, and upper vagina. It can occur in isolation (type 1) or in conjunction with various anomalies (type 2), with a subset of these comprising müllerian, renal, and cervicothoracic abnormalities (MURCS) association. The genetic causes of MRKH have been investigated previously yielding limited results, with massive parallel sequencing becoming increasingly utilized. We sought to identify genetic contributions to MRKH using a combination of microarray and whole exome sequencing (WES) on a cohort of 8 unrelated women with MRKH and MURCS. WES data were analysed using a candidate gene approach to identify potential contributing variants. Microarray analysis identified a 0.6-Mb deletion in the previously implicated 16p11.2 region in a patient with MRKH type 2. WES revealed 16 rare nonsynonymous variants in MRKH candidate genes across the cohort. These included variants in several genes, such as LRP10 and DOCK4, associated with disorders with müllerian anomalies. Further functional studies of these variants will help to delineate their biological significance and expand the genotypic spectrum of MRKH.
Collision Tumor of Adenocarcinoma and Gastrointestinal Stromal Tumor in the Small Bowel
Authors: Saito Hitoshi , Osaka Yoshiaki , Tamura Kazuhiko , Kawakita Hideaki , Kobayashi Nao , Nagakawa Yuichi , Katsumata Kenji , Tsuchida Akihiko
Published: November 2018

We report a very rare case of collision tumor composed of primary adenocarcinoma of the jejunum and gastrointestinal stromal tumor (GIST). The patient was a 63-year-old man who visited our hospital for epigastralgia and vomiting. Abdominal computed tomography revealed a mass in the upper jejunum, with gastric and duodenal dilatation. Endoscopy of the small bowel showed a circumferential tumor in the upper jejunum, which was diagnosed as primary adenocarcinoma by tissue biopsy. Thereafter, partial resection of the small bowel from the third part of the duodenum over the upper jejunum was performed. A tumor colliding with the primary adenocarcinoma was identified on the serosal side of the jejunum in the excised specimen and was histologically diagnosed as GIST. The annual incidence of primary adenocarcinoma of the small bowel (i.e., jejunum and ileum excluding the duodenum) has been reported to be 7 in 1 million people, and only 6 cases of collision tumor of the small bowel (i.e., duodenum: 5, ileum: 1) have been reported thus far. Although esophageal, gastric, and large intestinal collision tumors composed of primary cancer and GIST have been reported, to our knowledge, the present patient is the first case of the small bowel. The cause of or correlation between 2 tumors forming a collision tumor remains unclear. In the present patient, there was no pathological finding of infiltration between the 2 tumors. Although the collision of the 2 tumors was unclear, the findings indicate their independent development in closely located regions consistent with collision tumors.
Potential Blood Pressure Goals in IgA Nephropathy: Prevalence, Awareness, and Treatment Rates in Chronic Kidney Disease Among Patients with Hypertension in China (PATRIOTIC) Study
Authors: Zheng Ying , Wang Yong , Liu Shuwen , Wu Jie , Duan Shuwei , Zhu Hanyu , Wu Di , Cai Guangyan , Chen Xiangmei
Published: November 2018

Background/Aims: IgA nephropathy is the most prevalent form of primary glomerulonephritis worldwide. Among patients with kidney disease, hypertension is one of the most important risk factors of disease progression. Considering the limited evidence regarding the appropriate blood pressure (BP) goal for patients with IgA nephropathy, our aim was to critically appraise the potential BP goal in IgA nephropathy. Methods: We performed a retrospective analysis of the BP data from 1055 patients with IgA nephropathy, extracted from the database of a nationwide, multi-center, cross-sectional study, including 61 tertiary hospitals in China. Hypertension was defined by a BP ≥140/90 mmHg. Three BP cutoff levels were evaluated as control values: < 140/90 mmHg, < 130/80 mmHg and < 125/75 mmHg. The primary outcome of our study was the prevalence of BP control among patients with a 24-h proteinuria < 1 g/d or ≥ 1 g/d. Multivariate logistic regression analysis was used to identify demographic and clinical factors associated with a decrease in renal function for the different target levels of BP. Results: The overall prevalence of hypertension was 63.3%. BP was controlled under 140/90 mmHg in 49.1% of patients, with 34.3% of patients with proteinuria < 1 g/d reaching the target BP < 130/80 mmHg and only 12.9% of patients with proteinuria > 1 g/d achieving a BP < 125/75 mmHg. Among patients with proteinuria < 1 g/d, the adjusted odds ratios (OR) and 95% confidence interval (95% CI) of a decrease in renal function, for the 3 target BP levels, were as follows (P > 0.05): < 140/90 mmHg, 0.9 (0.5 - 1.6); < 130/80 mmHg, 1.0 (0.5 - 1.8); and < 125/75 mmHg, 1.0 (0.5 - 2.0). With proteinuria ≥1 g/d, the adjusted ORs (95%CI) of attaining the BP targets of < 140/90 mmHg, < 130/80 mmHg and < 125/75 mmHg were 0.4 (0.2 - 0.6), 0.2 (0.1 - 0.4) and 0.3 (0.1 - 0.5), respectively (P < 0.05). Conclusion: Hypertension was common in IgA nephropathy and hypertensive control was suboptimal. Our result supports a benefit of intensive control of BP < 130/80 mmHg for patients with proteinuria ≥1 g/d. However, in patients with proteinuria < 1 g/d, a renoprotective effect of this BP goal was not identified.
Clinical Manifestation, Management and Prognosis of Acute Myocardial Infarction in Autosomal Dominant Polycystic Kidney Disease
Authors: Yang Bo , Wang Qi , Wang Rui , Xu Tao
Published: November 2018

Background/Aims: Cardiovascular complications are the most common cause of death in individuals with autosomal dominant polycystic kidney disease (ADPKD), yet there is no substantial data concerning the clinical characteristics of acute myocardial infarction (AMI) in this population. This study thus aimed to investigate AMI in persons with ADPKD. Methods: A retrospective analysis of ADPKD patients admitted to our hospital over a 13 year period was conducted. Age and gender-matched control patients without ADPKD were also selected at a ratio of 1: 10. Results: A total of 52 ADPKD and 520 non-ADPKD patients were enrolled in the present study, with those in the former group exhibiting significantly poorer kidney function. The distribution of AMI types differed significantly between these two groups. The incidence of ST-segment elevation myocardial infarction (STEMI) was higher (75.0%) and the incidence of non-ST segment elevation myocardial infarction (NSTEMI) was lower (25.0%) in the ADPKD group. At the onset of AMI, sudden cardiac death (SCD) was more common in ADPKD patients (11.5% vs. 4.6%). In terms of risk factors, the occurrence of hypertension was greater in ADPKD patients (78.8% vs. 39.6%). With regard to subsequent management, ADPKD patients had a higher prevalence of triple-branch coronary lesions (21.1% vs. 11.2%), undergoing more coronary artery bypass grafting (CABG) (7.7% vs. 5.4%) and fewer percutaneous coronary interventions (PCI) (73.1% vs. 84.6%). Overall, ADPKD patients had higher rates of mortality (13.5% vs. 6.2%). Conclusion: ADPKD patients with AMI suffer from more severe conditions and difficult therapies, resulting in a poorer prognosis.
Hemodialysis or Peritoneal Dialysis, Which Is Better for Patients with Delayed Graft Function?
Authors: Yan Ting , Peng Wenhan , Lv Junhao , Wu Jianyong , Chen Jianghua
Published: November 2018

Background/Aims: Hemodialysis (HD) or peritoneal dialysis (PD) is an important renal replacement method in patients with delayed graft function (DGF) after kidney transplantation; however, it is not clear which dialysis modality is superior. This study determined the impact of different dialysis modalities on patients with DGF. Methods: It was a single-center, retrospective and descriptive study. We performed 673 kidney transplants from donors after cardiac death (DCD) between January 2010 and December 2016 at our center and 138 (20.5%) recipients developed DGF after transplantation. We classified the recipients into two groups according to post-transplant dialysis: DGF-HD (n=96) and DGF-PD (n=42). We analyzed the outcomes of the different dialysis modalities 30 days and 1 year post-transplantation. Results: There were no differences in baseline factors between patients with post-transplant HD (n = 96) or PD (n = 42). There were 10 patients with conversion from PD to HD during DGF. The DGF-PD patients had a higher rate of treatment failure than the DGF-HD patients (23.8% vs. 0%, p < 0.001), peritonitis (7.1% vs. 0%, p = 0.027), and longer duration of dialysis dependence (10.5 vs. 9 days, p = 0.003). There was no statistically significant difference between both groups with respect to acute rejection, hemorrhage, and patient and graft survival at 1 year. Conclusion: In renal transplant recipients with DGF, post-transplant PD led to increased treatment failure. PD did not result in rapid recovery of transplanted renal function, and had a high probability of peritonitis.
The Role of Intestinal Fatty Acid Binding Proteins in Protecting Cells from Fatty Acid Induced Impairment of Mitochondrial Dynamics and Apoptosis
Authors: Sarkar-Banerjee Suparna , Chowdhury Sourav , Sanyal Dwipanjan , Mitra Tulika , Roy Sib Sankar , Chattopadhyay Krishnananda
Published: November 2018

Background/Aims: The conformation, folding and lipid binding properties of the intestinal fatty acid binding proteins (IFABP) have been extensively investigated. In contrast, the functional aspects of these proteins are not understood and matter of debates. In this study, we aim to address the deleterious effects of FA overload on cellular components, particularly mitochondria; and how IFABP helps in combating this stress by restoring the mitochondrial dynamics. Methods: In the present study the functional aspect of IFABP under conditions of lipid stress was studied by a string of extensive in-cell studies; flow cytometry by fluorescence-activated cell sorting (FACS), confocal imaging, western blotting and quantitative real time PCR. We deployed ectopic expression of IFABP in rescuing cells under the condition of lipid stress. Again in order to unveil the mechanistic insights of functional traits, we arrayed extensive computational approaches by means of studying centrality calculations along with protein-protein association and ligand induced cluster dissociation. While addressing its functional importance, we used FCS and in-silico computational analyses, to show the structural distribution and the underlying mechanism of IFABP’s action. Results: Ectopic expression of IFABP in HeLa cells has been found to rescue mitochondrial morphological dynamics and restore membrane potential, partially preventing apoptotic damage induced by the increased FAs. These findings have been further validated in the functionally relevant intestinal Caco-2 cells, where the native expression of IFABP protects mitochondrial morphology from abrogation induced by FA overload. However, this native level expression is insufficient to protect against apoptotic cell death, which is rescued, at least partially in cells overexpressing IFABP. In addition, shRNA mediated IFABP knockdown in Caco-2 cells compromises mitochondrial dynamics and switches on intrinsic apoptotic pathways under FA-induced metabolic stress. Conclusion: To summarize, the present study implicates functional significance of IFABP in controlling ligand-induced damage in mitochondrial dynamics and apoptosis.
Morin Exhibits Anti-Inflammatory Effects on IL-1β-Stimulated Human Osteoarthritis Chondrocytes by Activating the Nrf2 Signaling Pathway
Authors: Qu Yanlong , Wang Chunlei , Liu Ning , Gao Chengzhe , Liu Fei
Published: November 2018

Background/Aims: Osteoarthritis (OA) is a multifactorial disease that is associated with inflammation in joints. The purpose of the present study was to investigate the anti-inflammatory activity and mechanism of morin on human osteoarthritis chondrocytes stimulated by IL-1β. Methods: The levels of NO and PGE2 were measured by the Griess method and ELISA. The levels of MMP1, MMP3, and MMP13 were also measured by ELISA. Results: The results revealed that IL-1β significantly increased the production of NO, PGE2, MMP1, MMP3, and MMP13. Additionally, the increases were significantly attenuated by treatment with morin. Furthermore, IL-1β-induced NF-κB activation was suppressed by morin. In addition, the expression of Nrf2 and HO-1 were increased by morin and knockdown of Nrf2 could prevent the anti-inflammatory effects of morin. Conclusion: In conclusion, this study suggested that morin attenuated IL-1β-induced inflammation by activating the Nrf2 signaling pathway.
Ciliary Neurotrophic Factor (CNTF) Protects Myocardial Cells from Oxygen Glucose Deprivation (OGD)/Re-Oxygenation via Activation of Akt-Nrf2 Signaling
Authors: Zheng Koulong , Zhang Qing , Sheng Zhenqiang , Li Yefei , Lu Hui-he
Published: November 2018

Background/Aims: Oxygen glucose deprivation (OGD)/re-oxygenation (OGDR) exposure to myocardial cells mimics ischemia-reperfusion injuries. We studied the potential activity of ciliary neurotrophic factor (CNTF) on OGDR-treated myocardial cells. Methods: CNTF and CNTFR expression were tested by RT-PCR assay and Western blotting assay. Cell viability and death were tested by MTT assay and LDH release assay, respectively. Akt-Nrf2 signalings were tested by Western blotting assay and qPCR assay. Results: CNTF and its receptor CNTFR were functionally expressed in established H9c2 myocardial cells and primary murine myocardiocytes. Pretreatment of CNTF significantly attenuated OGDR-induced viability reduction and death in myocardial cells. Further studies show that in the myocardial cells CNTF activated NF-E2-related factor 2 (Nrf2) signaling to inhibit OGDR-induced reactive oxygen species (ROS) production and programmed necrosis, preventing adenine nucleotide translocator 1 (ANT-1)-p53-cyclophilin D (Cyp-D) mitochondrial association and mitochondrial depolarization. Nrf2 silencing or knockout almost abolished CNTF-induced H9c2 cytoprotection against OGDR. CNTF activated Akt in H9c2 cells and primary murine myocardiocytes. Conversely, Akt blockage by the pharmacological inhibitors not only blocked CNTF-induced Nrf2 Ser-40 phosphorylation and activation, but also nullified anti-OGDR actions by CNTF in myocardial cells. Conclusion: CNTF activates Akt-Nrf2 signaling to protect myocardial cells from OGDR.
Cr(VI)-Induced Autophagy Protects L-02 Hepatocytes from Apoptosis Through the ROS-AKT-mTOR Pathway
Authors: Liang Qi , Xiao Yuanyuan , Liu Kaihua , Zhong Caigao , Zeng Ming , Xiao Fang
Published: November 2018

Background/Aims: Hexavalent chromium [Cr(VI)] pollution has become a global concern for both ecosystems and human health. Our previous study revealed Cr(VI) could induce both apoptosis and autophagy in L-02 hepatocytes. Here, we sought to explore the underlying mechanism of Cr(VI)-induced autophagy and its exact role in cell death. Methods: Autophagy ultrastructure was observed under transmission electron microscope (TEM), autophagy flux was measured with double-tagged mCherry-green fluorescent protein (GFP)-microtubule-associated protein 1 light chain 3 (LC3) assay, long-lived protein degradation assay, and LC3II expression assay in the presence of lysosomal inhibitor, bafilomycin A1 (BafA1). Reactive oxygen species (ROS) level was determined using fluorescent probe dichloro-dihydrofluorescein diacetate (DCFH-DA). The expression levels of Beclin-1, LC3, p62/ SQSTM1, and AKT-mammalian target of rapamycin (mTOR) pathway-related molecules including phosphorylation (p)-AKT, AKT, p-mTOR, and mTOR were examined using real-time polymerase chain reaction (RT-PCR) and western blotting. Apoptosis was determined using Annexin V- fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining. Results: Our results demonstrated Cr(VI) exposure activated autophagy in L-02 hepatocytes, as evidenced by the accumulation of autophagosomes, the increase of LC3-II and degradation of p62/ SQSTM1, and the enhanced overall degradation of proteins. We also confirmed Cr(VI)-induced LC3-II elevation mainly came from autophagy induction rather than lysosomal degradation impairment. ROS-AKT-mTOR pathway was associated with Cr(VI)-induced autophagy, and ROS scavenger N-acetylcysteine (NAC) pretreatment inhibited Cr(VI)-induced autophagy by alleviating the inhibition of the AKT-mTOR pathway. Autophagy inhibitors 3-methyladenine (3-MA) and chloroquine diphosphate (CDP) promoted Cr(VI)-induced apoptotic death. Conclusion: These findings indicated Cr(VI)-induced autophagy protected L-02 hepatocytes from apoptosis through the ROS-AKT-mTOR pathway.
K6PC-5 Activates SphK1-Nrf2 Signaling to Protect Neuronal Cells from Oxygen Glucose Deprivation/Re-Oxygenation
Authors: Liu Hua , Zhang Zhiqing , Xu Min , Xu Rong , Wang Zhichun , Di Guangfu
Published: November 2018

Background/Aims: New strategies are required to combat neuronal ischemia-reperfusion injuries. K6PC-5 is a novel sphingosine kinase 1 (SphK1) activator whose potential activity in neuronal cells has not yet been tested. Methods: Cell survival and necrosis were assessed with a Cell Counting Kit-8 assay and lactate dehydrogenase release assay, respectively. Mitochondrial depolarization was tested by a JC-1 dye assay. Expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) signaling components were examined by quantitative real-timePCR and western blotting. Results: K6PC-5 protected SH-SY5Y neuronal cells and primary murine hippocampal neurons from oxygen glucose deprivation/re-oxygenation (OGDR). K6PC-5 activated SphK1, and SphK1 knockdown by targeted short hairpin RNA (shRNA) almost completely abolished K6PC-5-induced neuronal cell protection. Further work showed that K6PC-5 inhibited OGDR-induced programmed necrosis in neuronal cells. Importantly, K6PC-5 activated Nrf2 signaling, which is downstream of SphK1. Silencing of Nrf2 by targeted shRNA almost completely nullified K6PC-5-mediated neuronal cell protection against OGDR. Conclusion: K6PC-5 activates SphK1-Nrf2 signaling to protect neuronal cells from OGDR. K6PC-5 might be a promising neuroprotective strategy for ischemia-reperfusion injuries.
Neurokinin 3 Receptor Antagonism: A Novel Treatment for Menopausal Hot Flushes
Authors: Modi Manish , Dhillo Waljit S.
Published: November 2018

Menopause is associated with significant symptomatic burden, with approximately two-thirds of postmenopausal women suffering from vasomotor symptoms, hot flushes, and night sweats. The mainstay of treatment for hot flushes continues to be hormone replacement therapy. However, as hormone replacement therapy is contraindicated in some cases, alternative, efficacious treatment options are also required. Hot flushes are thought to arise as a result of significant changes in the neuroendocrine circuitry underpinning the reproductive axis during menopause. This includes reduced circulating ovarian oestrogens, hypersecretion of gonadotropins, and increased expression of kisspeptin and neurokinin B (NKB) within the infundibular nucleus of the hypothalamus. In recent years, NKB, predominantly acting via the neurokinin 3 receptor (NK3R), has emerged as an important player in the development of menopausal hot flushes. Antagonism of NK3R has garnered much interest as a novel therapeutic target to help ameliorate hot flush symptoms. Improvements in hot flush frequency, severity, and quality of life have been demonstrated in a number of clinical trials using novel NK3R antagonists in postmenopausal women. Within this review, we will explore the growing body of evidence supporting antagonism of NK3R as a potentially promising treatment for menopausal hot flushes.
ВЗАЄМОЗВ'ЯЗОК ДИСФУНКЦІЇ СКРОНЕВО-НИЖНЬОЩЕЛЕПНОГО СУГЛОБУ З ПОРУШЕННЯМ ПОСТАВИ У ЛЮДЕЙ МОЛОДОГО ВІКУ
Authors: Tabaka K. I. , Bakalyuk T. G. , Sirant H. O.
Published: November 2018

На сьогодні захворювання скронево-нижньощелепного суглоба є поширеною патологією щелепно-лицьової ділянки. Проведено аналіз взаємозв’язку дисфункції скронево-нижньощелепного суглоба з порушенням постави у пацієнтів  молодого віку.
Lectotypifications in Oxalis (Oxalidaceae) for the Flora of Argentina
Authors: LÓPEZ ALICIA , SASSONE AGOSTINA B. , FIASCHI PEDRO
Published: November 2018

As part of a revision of Oxalis for the Flora Argentina, the types of all the relevant names for the country were assessed. Seven names were inadvertently lectotypified before 2001, but require second-step lectotypifications. As a whole, eleven lectotypes (first and second step) are designated here.
Lactarius taedae (Russulales): an unexpected new gasteroid fungus from Brazil
Authors: SILVA-FILHO ALEXANDRE G. S. , SULZBACHER MARCELO A. , FERREIRA RENATO J. , BASEIA IURI G. , WARTCHOW FELIPE
Published: November 2018

Data about the occurrence of basidiomata forming hypogeous sequestrate fungi in Brazil are limited, and studies on this group are needed. During studies of hypogeous sequestrate fungi in Rio Grande do Sul State, Brazil, an unexpected new species of Lactarius was sampled from soils of an exotic Pinus plantation and described based on molecular and morphological data. This new species is recognized by the reddish yellow to dark brown, hypogeous basidiomes with a persistent peridium, white unchanging latex, globose to broadly ellipsoid basidiospores that are ornamented with a complete and dense reticulum and the peridiopellis composed by a cutis of repent firmly woven hyphae.
New findings of rare or little-known alpheid shrimp genera (Crustacea, Decapoda) in Moorea, French Polynesia
Authors: ANKER ARTHUR
Published: November 2018

Six rare or little-known genera from the shrimp family Alpheidae are reported from French Polynesia, five of them for the first time. Distribution ranges are significantly extended for the recently described Acanthanas pusillus Anker, Poddoubtchenko & Jeng, 2006 and Richalpheus palmeri Anker & Jeng, 2006, previously known only from two and one type specimens, respectively, from the Philippines. The original description of A. pusillus, based on two females, is completed with the description and illustration of the male cheliped and pleopod; accordingly, the diagnosis of Acanthanas is slightly emended. The genus Leptalpheus Williams, 1965 was not known from French Polynesia before, but is represented there by two species: L. denticulatus Anker & Marin, 2009, previously known from Vietnam, the Philippines and Fiji; and L. pacificus Banner & Banner, 1974, previously considered as a Hawaiian endemic. The genus Prionalpheus Banner & Banner, 1960 is represented in French Polynesia by two species: P. triarticulatus Banner & Banner, 1960, reported for the first time, and P. brachytomeus Banner & Banner, 1971, reported with some doubts (P. cf. brachytomeus) for the first time since the original description. Rugathanas borradailei (Coutière, 1903), a widespread but rather uncommon and easily overlooked species, is also reported for the first time from French Polynesia. Colour patterns are illustrated for all species, this being for the first time for R. palmeri, L. pacificus, P. triarticulatus and P. cf. brachytomeus.
A new genus of calcareous sponge discovered in the Caribbean Sea: Bidderia gen. nov. (Porifera, Calcarea, Calcinea)
Authors: LOPES MATHEUS V. , CÓNDOR-LUJÁN BÁSLAVI , AZEVEDO FERNANDA , PÉREZ THIERRY , KLAUTAU MICHELLE
Published: November 2018

Calcareous sponges from the Lesser Antilles were recently inventoried and several specimens morphologically resembling species of the genus Ascoleucetta were collected. Morphological and molecular (C-LSU and ITS) analyses indicated that these specimens from the Lesser Antilles constituted a new genus. They lack the conspicuous and very characteristic ornamentation of the inhalant apertures found in two out of three species of Ascoleucetta, including the type species A. compressa. In the molecular analyses, the specimens clustered as an independent lineage, distant from the clade of A. compressa. Based on these results, we decided to erect a new genus, Bidderia gen. nov., whose type species is Bidderia bicolora gen. nov. sp. nov. Considering this new discovery, we are proposing to transfer the species A. amitsba to the genus Bidderia gen. nov. and to rediagnose Ascoleucetta.
Snake eels of the genus Ophichthus (Anguilliformes: Ophichthidae) from Myanmar (Indian Ocean) with the description of two new species
Authors: McCOSKER JOHN E. , PSOMADAKIS PETER N.
Published: November 2018

Two new species of ophichthid eels, subfamily Ophichthinae, are described and illustrated from specimens collected from Myanmar by the R/V Dr. Fridtjof Nansen. Included are: Ophichthus nansen sp. nov., from 103–106 m depth, which is unique in its vertebral number (11-53-116), dorsal-fin origin (above mid-pectoral fin), jaw dentition (small, conical and mostly biserial), and coloration (dark gray-brown, fins black); and Ophichthus naga sp. nov., from 455–459 m depth, which is unique in its vertebral number (15-65-153), dorsal-fin origin (well behind pectoral fin), dentition (small, conical, biserial in upper jaw and uniserial in lower jaw and on vomer), and dark brown coloration. Also new to Myanmar are Ophichthus cephalozona Bleeker, 1864, and O. lithinus (Jordan & Richardson, 1908), which are diagnosed and treated herein.
Nonlinear Wave Ensemble Averaging in the Gulf of Mexico Using Neural Networks
Authors: Campos Ricardo Martins , Krasnopolsky Vladimir , Alves Jose-Henrique G. M. , Penny Stephen G.
Published: November 2018

Artificial neural networks (ANNs) applied to nonlinear wave ensemble averaging are developed and studied for Gulf of Mexico simulations. It is an approach that expands the conservative arithmetic ensemble mean (EM) from the NCEP Global Wave Ensemble Forecast System (GWES) to a nonlinear mapping that better captures the differences among the ensemble members and reduces the systematic and scatter errors of the forecasts. The ANNs have the 20 members of the GWES as input, and outputs are trained using observations from six buoys. The variables selected for the study are the 10-m wind speed (U10), significant wave height (Hs), and peak period (Tp) for the year of 2016. ANNs were built with one hidden layer using a hyperbolic tangent basis function. Several architectures with 12 different combinations of neurons, eight different filtering windows (time domain), and 100 seeds for the random initialization were studied and constructed for specific forecast days from 0 to 10. The results show that a small number of neurons are sufficient to reduce the bias, while 35–50 neurons produce the greatest reduction in both the scatter and systematic errors. The main advantage of the methodology using ANNs is not on short-range forecasts but at longer forecast ranges beyond 4 days. The nonlinear ensemble averaging using ANNs was able to improve the correlation coefficient on forecast day 10 from 0.39 to 0.61 for U10, from 0.50 to 0.76 for Hs, and from 0.38 to 0.63 for Tp, representing a gain of five forecast days when compared to the EM currently implemented.
Perceived Discrimination, Ethnic Identity, and Psychological Well-Being Among Arab Americans
Authors: Atari Rawan , Han Suejung
Published: November 2018

In this study, we examined associations among perceived discrimination, ethnic identity dimensions (exploration, resolution, affirmation), and psychological well-being (self-esteem, life satisfaction, flourishing) among 156 Arab Americans. Multiple moderated regression analyses revealed that perceived discrimination was negatively associated, and ethnic identity resolution and affirmation were positively associated, with psychological well-being. Ethnic identity affirmation moderated the association between perceived discrimination and flourishing by buffering the negative effect of perceived discrimination. Ethnic identity resolution and exploration did not moderate the associations between perceived discrimination and psychological well-being. Affirmation both promoted and protected well-being, resolution promoted well-being, and exploration was not associated with promoting or protecting well-being. We discuss implications to help Arab American clients develop and capitalize on their ethnic identity, promote their well-being, and buffer against perceived discrimination.