bluuebunny/crossref_metadata_2025 huggingface dataset:
Rancang Bangun Alat Kontrol Lampu Jarak Jauh Menggunakan Mikrokontroler Berbasis Website
Authors: Frare Laban Yoel Ishak , Ramos Samuel
Published: March 2023

It is very important to pay attention to the management of electrical devices in spaces for human activity related to the specified time when the time is on and when it is off to avoid wasting energy using wasted energy. There are several procedures for managing electricity that has been implemented using renewable technology in the current era of industrial trends 4.0, including controlling lights and other electrical devices. In general, light control uses manual procedures by turning on the switch directly, starting from offices, shopping centers, public places and others. In this modern era, it is possible for humans to use technological updates by changing the use of light switches to become automatic using a microcontroller control board and a wifi module as a transmission medium that is integrated with the web as a portal for monitoring light points installed in a building or facilities. general. This study aims to obtain new habits of the community regarding the use of automatic light control devices in order to save on the cost of using electricity. This change is also beneficial for everyone who is outside the house in their daily life, so they don't have to go home when they want to turn on the lights. By using the website platform, electrical devices located in different rooms can be controlled via cell phones and can also be controlled via computers or gadgets.
Resenha: Teoria de moda: sociedade, imagem e consumo
Authors: Novelli Daniela , Sena Taísa Vieira
Published: March 2023

Mara Rúbia Sant'Anna é autora da obra Teoria de moda: sociedade, imagem e consumo, publicada no Brasil em 2007, pela editora Estação das Letras. Situando o discurso sobre a moda na dimensão presentista do mundo contemporâneo, através de um estudo consistente, a autora relaciona de forma inovadora modernidade, moda e aparência, qualificando e caracterizando a sociedade contemporânea ocidental como uma sociedade de moda.
Fast fashion: estratégia para conquista do mercado globalizado
Authors: Delgado Daniela
Published: March 2023

O conceito de fast fashion se torna cada vez mais importante para o varejo de moda mundial que visa atender aos indivíduos da atualidade, cada vez mais ávidos pelo consumo e mais informados em termos de moda. A Zara foi uma das primeiras a introduzir o fast fashion como estratégia de vendas que tem se mostrando como uma forma dinâmica de crescimento do faturamento da empresa e de seu reconhecimento no mercado global.
Sustentabilidade ambiental: um desafio para a moda
Authors: Schulte Neide Köhler , Lopes Luciana Dornbusch
Published: March 2023

Neste artigo se propõe uma reflexão sobre um paradigma que se estabeleceu no século XXI: ‘o desenvolvimento ambientalmente sustentável’, e sua implicação na criação de produtos para o vestuário. O consumidor, a indústria, o criador de novos produtos, todos têm papéis determinantes na consolidação deste paradigma. Os impactos ambientais devem ser considerados em todas as etapas nos projetos de novos produtos, da origem da matéria-prima até o descarte pelo consumidor. O desenvolvimento sustentável é um grande desafio para a criação de novos produtos para o vestuário de moda, pois o ciclo de vida muito curto destes produtos, e o apelo ao consumismo, representam um entrave. Diante deste contexto, são identificados novos cenários para a moda.
O estilo enquanto lógica de identificação: elo entre as características expressivas complexas que se coadunam no trânsito do processo histórico e a manifestação expressiva particular e singular de um indivíduo
Authors: Tronca Flávia Zambon
Published: March 2023

O estilo é expressão da individualidade, personalíssimo, e tem a sua própria linguagem e ritmo, porém, é invisível ao primeiro olhar. É síntese da experiência de um sujeito tornando-se identidade pessoal. Porém, apesar de ser único, pessoal e intransferível o estilo estabelece inúmeras combinações com as expressões cristalizadas de muitos outros. Ao mesmo tempo que é marca de si, é, também, espírito do tempo em toda a sua complexidade.
Artesanato e Moda: uma contribuição ao processo
Authors: Clemencio Maria Aparecida
Published: March 2023

O objetivo deste artigo é propor uma reflexão sobre a utilização de produtos artesanais como fator de diferenciação e agregação de valor às vestimentas e aos acessórios. Busca, ainda, entender de que forma os produtos artesanais podem ser inseridos em uma produção industrial massificada.
Ateliê e educação a distância: uma ação completar ao ensino superior presencial de moda
Authors: Lopes Luciana Dornbusch , Varella Leah , Gonçalves Marília Matos , Gonçalves Eliana , Carioni Carolina
Published: March 2023

Este trabalho tem como objetivo apresentar o desenvolvimento de uma videoaula experimental criada para inclusão em AVEA – Ambiente Virtual de Ensino Aprendizagem para o ensino superior de Moda como complemento às aulas presenciais da disciplina de Ateliê Experimental de Confecção do curso de bacharelado em Moda – habilitação em Design de Moda do CEART/UDESC. Apresenta as conceituações e caracterização da Educação a Distância além de um sistema para gerenciamento de cursos – o Moodle6, bem como a atividade do infodesign no processo de criação da videoaula. Como tema da videoaula, escolheu-se a apresentação da confecção da camisa masculina. A videoaula foi desenvolvida com base na metodologia proposta por Ligia C. Girao.
Da alta costura ao prêt-à-porter, da fast fashion a slow fashion: um grande desafio para a Moda
Authors: Refosco Ereany , Oenning Josiany , Neves Manuela
Published: March 2023

Depois da Alta Costura, do abandono das roupas feitas artesanalmente e sob medida, e com o início do Prêt-à-porter, a moda passou por diversas transformações. Entre elas, o desenvolvimento de técnicas produtivas, novas tecnologias, novas matérias primas e, também, a introdução de importantes produtos no cotidiano. A moda gerou riqueza, colocou a indústria têxtil e os designers em evidência. Envolve aspectos psicológicos, financeiros e sociológicos. Incorpora os desejos e necessidades dos consumidores. Em pleno século XXI, a análise de toda esta cadeia produtiva perpassa os aspectos sustentáveis. A partir desta percepção, vê-se que os papéis desempenhados pelo consumidor, pelo fabricante e pelo designer devem considerar o impacto da produção no meio e o ciclo de vida do produto. Neste momento, a moda pode estar caminhando da fast fashion para slow fashion quando, finalmente, além dos objetivos monetários, o foco maior estará no design, na qualidade, na durabilidade dos produtos e também no ambiente.
Informação através da cor: a construção simbólica psicodinâmica das cores na concepção do produto
Authors: Xavier Nunes Ana Camila Nobre
Published: March 2023

Considerando a amplitude e importância da cor em diversas áreas, este artigo objetiva demonstrar a relevância do estudo das cores aplicado ao processo que engloba a geração de um produto (desde a embalagem utilizada, à publicidade para divulgação do produto). Busca-se evidenciar a capacidade informativa das cores, através da análise dos estimulos, da percepção e da sintaxe das cores, assim como através da sua utilização simbólica e linguística na transmissão de mensagens e informação, revelando, também, a influência dos elementos psicodinâmicos na escolha do consumidor e a importância de análises do mercado e pesquisa de tendências na concepção do produto.
Uniforme escolar infantil: seu posicionamento na identificação dos gêneros
Authors: Rabelo Andrade Raquel , Pereira Lívia
Published: March 2023

O uniforme escolar faz parte do cotidiano de crianças estudantes brasileiras desde meados do século XX e ainda hoje continua sendo um artefato amplamente utilizado, tanto por escolas públicas como particulares. Desse modo, algumas considerações e análises podem ser feitas em relação à representação e identificação dos gêneros no uniforme a fim de se verificar sua contribuição na constituição das identidades. Nesse sentido, este estudo busca investigar, por meio de pesquisa bibliográfica e análise imagética, a relação que se estabelece entre a identificação dos gêneros e os uniformes escolares infantis, desde seu advento até a atualidade.
Impact of interventions for tuberculosis prevention and care in South Africa – a systematic review of mathematical modelling studies
Authors: Brown LK , Van Schalkwyk C , De Villiers AK , Marx FM
Published: March 2023

Background. Substantial additional efforts are needed to prevent, find and successfully treat tuberculosis (TB) in South Africa (SA). In thepast decade, an increasing body of mathematical modelling research has investigated the population-level impact of TB prevention and careinterventions. To date, this evidence has not been assessed in the SA context.Objective. To systematically review mathematical modelling studies that estimated the impact of interventions towards the World HealthOrganization’s End TB Strategy targets for TB incidence, TB deaths and catastrophic costs due to TB in SA.Methods. We searched the PubMed, Web of Science and Scopus databases for studies that used transmission-dynamic models of TB in SAand reported on at least one of the End TB Strategy targets at population level. We described study populations, type of interventions andtheir target groups, and estimates of impact and other key findings. For studies of country-level interventions, we estimated average annualpercentage declines (AAPDs) in TB incidence and mortality attributable to the intervention.Results. We identified 29 studies that met our inclusion criteria, of which 7 modelled TB preventive interventions (vaccination,antiretroviral treatment (ART) for HIV, TB preventive treatment (TPT)), 12 considered interventions along the care cascade for TB(screening/case finding, reducing initial loss to follow-up, diagnostic and treatment interventions), and 10 modelled combinationsof preventive and care-cascade interventions. Only one study focused on reducing catastrophic costs due to TB. The highest impactof a single intervention was estimated in studies of TB vaccination, TPT among people living with HIV, and scale-up of ART. Forpreventive interventions, AAPDs for TB incidence varied between 0.06% and 7.07%, and for care-cascade interventions between 0.05%and 3.27%.Conclusion. We describe a body of mathematical modelling research with a focus on TB prevention and care in SA. We found higherestimates of impact reported in studies of preventive interventions, highlighting the need to invest in TB prevention in SA. However, studyheterogeneity and inconsistent baseline scenarios limit the ability to compare impact estimates between studies. Combinations, rather thansingle interventions, are likely needed to reach the End TB Strategy targets in SA
Melanoma crude incidence rates among White South Africans climbing as in other countries: urgent call for targeted skin cancer prevention and awareness campaigns
Authors: Wright Caradee , Kapwata Thandi , Albers Patricia
Published: March 2023

[We have not prepared an abstract for the editorial but we can do so if required.]
Investigación artística en la educación superior: contexto de inclusión y modalidades
Authors: Galarza-Neira María Teresa
Published: March 2023

En primera instancia, este artículo pretende aproximarse a la reconfiguración de la educación superior, en el marco del Proceso de Bolonia en Europa, y sus iridiscencias en otros territorios. El texto contextualiza en este escenario una reflexión sobre la inclusión de la investigación artística en la academia. A continuación, se ofrece una exploración de las diversas modalidades que asume la investigación artística en la esfera universitaria. Para ello, a partir de las propuestas de Christopher Frayling, Henk Borgdorff y Lyle Skains, se marcan tres momentos en la configuración de esas modalidades, establecidas a partir de los modos de relación entre el arte y la investigación, según los cuales la primera puede ocupar el rol de objeto o método de la segunda, aunque también puede constituir el propio proceso-resultado de investigación.
In vitro evaluation of cytotoxicity of different brands of artificial teeth
Authors: Gusmão João Milton Rocha , Pithon Matheus Melo , Santos Rogerio Lacerda dos , Romanos Maria Teresa Villele
Published: March 2023

Aim: The present study aimed to evaluate the cytotoxicity of different brands of stock acrylic teeth against fibroblast cells. Materials and Methods: For this experiment, the cell lineage used was the L929 from the American Type Culture Collection (ATCC, Rockville, MD, USA). The brands of stock teeth tested included: Artplus, Biolux, Biotone, Pop Dent, Vipi Dent, Orthosit Ivoclar, Biocler, and Trilux. Each group was placed in contact with the fibroblast cells for the periods of 24, 48, 72, and 168 hours. Control cells, as well as negative and positive controls, were used to compare the extremes. Results: The results showed cytotoxicity for all the groups tested in the time periods of 24, 48, and 72 hours during the experiment, which occurred in the Orthosit Ivoclar and Trilux groups even after the experiment had been conducted for 168 hours. Conclusion:  The researched acrylic teeth developed cytotoxicity against the cells in vitro during the time periods of 24, 48, and 72 hours, while Ivoclar Vivodent and Trilux brands of teeth developed cytotoxicity against the tested cells during the time period of 168 hours.Uniterms: Tooth artificial. Evaluation studies. Cell line.
Identificação de indivíduos por meio das marcas de mordida em alimentos utilizando a engenharia reversa e a prototipagem rápida: caso simulado
Authors: Nascimento Michelle Michel , Sarmento Viviane Almeida , Beal Valter Estevão , Galvão Luis Carlos Cavalcante , Marques Jeidson Antônio Morais
Published: March 2023

Objetivo: Este trabalho objetivou avaliar a viabilidade do uso da engenharia reversa e da prototipagem rápida para auxiliar na identificação de indivíduos através da marca de mordida em alimentos (chocolate e fatia de bolo). Materiais e Métodos: A amostra foi composta por oito voluntários. Por meio de sorteio, três voluntários foram definidos para serem os suspeitos. Posteriores sorteios foram feitos para que a mordida fosse realizada nos respectivos alimentos. Depois de definida a amostra os modelos dos suspeitos e os alimentos mordidos foram digitalizados utilizando o software Measuring System ATOS I - Industrial 3D Digitizer v6.1. Viewer-8 and TRITOP of GOM Inspect v1.2.1 (Germany). Gerado o arquivo das imagens, estas foramcomparadas através da análise tridimensional. A partir do modelo virtual dos alimentos mordidos estes dados foram enviados para uma impressora de prototipagem rápida onde foi confeccionado o protótipo dos alimentosmordidos. Posteriormente, foi feita a comparação direta do protótipo do alimento com o modelo de gesso dos suspeitos. Através da engenharia reversa e da prototipagem rápida foi possível identificar com precisãoo autor da mordida no alimento chocolate. Entretanto, o alimento fatia de bolo apresentou dificuldade para a correta identificação do suspeito. Resultados: Os resultados obtidos permitiram concluir que a textura doalimento bolo, bem como, sua fragilidade e consistência podem influenciar na obtenção das imagens 3D e consequente construção do protótipo. Conclusão: Novos estudos com outros tipos de alimentos necessitam serrealizados para verificar a aplicabilidade da prototipagem rápida, devido ao seu grande poder de confronto e, principalmente, pela capacidade de transformar uma evidência perecível em uma prova manipulável e perene.Descritores: Força de mordida. Odontologia legal.
Humanização no atendimento odontológico: acolhimento da subjetividade dos pacientes atendidos por alunos de graduação em Odontologia
Authors: Mota Luciane de Queiroz , Farias Danilo Barboza Lopes Magalhães , Santos Thalita Almeida dos
Published: March 2023

Objetivo: O objetivo do presente estudo foi avaliar a subjetividade dos pacientes, quanto ao seu atendimento por alunos de graduação; à existência de ansiedade odontológica; e ao perfil de um dentista ideal. Materiais e Métodos: A pesquisa, descritiva e exploratória, utilizou o método quanti-qualitativo, com análise de conteúdo. O instrumento de coleta de dados foi uma entrevista estruturada com perguntas objetivas e subjetivase a amostra foi constituída por 48 usuários da demanda espontânea das clinicas odontológicas da Universidade Federal da Paraíba, selecionada por conveniência. Foram incluídos na amostra os pacientes a partir de 12 anosde idade, de ambos os gêneros, sem distinção de raça, que procuraram os serviços odontológicos no período de novembro de 2009 a maio de 2010. Foram excluídos da pesquisa os pacientes portadores de necessidadesespeciais, que não tinham condições de interagir com o pesquisador. Foi identificado, em cada questão, o(s) núcleo(s) de sentido existente(s), de onde foram extraídos os indicadores visando a classificação nas categoriase a sua contagem, cujos resultados foram expressos através de freqüência e números absolutos. Resultados: A maioria dos entrevistados (56,3%) se sentia seguro ao serem atendidos por alunos de graduação; 41,7% dospacientes consideraram que um dentista ideal deveria ter capacidade técnica e ser atencioso; e 35% possuíam ansiedade odontológica. Conclusões: Os pacientes se sentem seguros e confiantes ao serem atendidos poralunos do curso de Graduação nas escolas de Odontologia e almejam que um dentista ideal seja um profissional com habilidades técnicas, mas, sobretudo, que tenham um contato humanizado e acolhedor com o paciente.A ansiedade odontológica ainda é um temor freqüente entre os pacientes, mas, acredita-se que num futuro próximo esse sentimento seja minimizado em virtudes das novas políticas de humanização nos serviços desaúde. Descritores: Ansiedade ao tratamento odontológico. Humanização da assistência. Acolhimento.
Urgências odontológicas em uma Unidade de Saúde vinculada à Estratégia Saúde da Família de Montes Claros, Minas Gerais
Authors: Pinto Eduardo Carneiro , Barros Vânia Julieta de Araújo , Coelho Mânia de Quadros , Costa Simone de Melo
Published: March 2023

Objetivo: Identificar os casos de urgência odontológica na Estratégia Saúde Família, em Montes Claros, Minas Gerais caracterizando o usuário pelo perfil sociodemográfico, de comportamento em saúde, estilo de vida e condição sistêmica. Materiais e Métodos: Trata-se de estudo transversal e quantitativo, de base documental. Foram avaliados 164 prontuários odontológicos, de adultos e idosos, atendidos entre 2008 e 2010. A análise dos dados foi por meio do teste χ2 de Pearson e pelo teste t de Student considerando o nível de significância p<0,05. Resultados: A idade média dos usuários foi de 35,0 anos (±12,9), sem diferença estatística entre os sexos (p=0,06). As mulheres (63,4%) e os alfabetizados (97,6%) utilizaram mais o serviço de urgência. A presença de alguma doença sistêmica foi registrada em 22,6% dos documentos. A média de escovação dental diária foi de 2,73 (±8,15), com diferença significativa para etilismo e sexo (p<0,05). A maioria dos homens e das mulheres não faz uso de fio dental (p=0,15). Cerca de 37,0% dos pacientes concluíram o tratamento, não sendo associada à condição sistêmica, ao tabagismo e ao etilismo (p>0,05). A dor foi a principal queixa que motivou a procura pelo serviço, sendo a cárie o diagnóstico mais frequente. Conclusão: A maioria das pessoas que procurou pelo serviço de urgência odontológica é do sexo feminino e possui idade média de 35 anos. Acárie foi o problema mais constatado, sendo a procura pelo serviço motivada, principalmente, pela dor.Descritores: Identificação de emergência. Odontologia. Saúde Pública.
Perfil epidemiológico de cegueira e perda do globo ocular por traumatismos em pacientes reabilitados através de próteses
Authors: Cardoso Silvana Orestes- , Magalhães Thaíza de Souza , Almeida Jefferson Luis Freire de , Massa Mirella Emerenciano , Vieira Fernando Luiz Tavares
Published: March 2023

Objetivo: Determinar o perfil epidemiológico de cegueira e perda do globo ocular por traumatismos, considerando-se a prevalência e a etiologia dos traumas oculares, segundo variáveis sociodemográficas e econômicas, assim como os tipos de agentes que causaram a lesão, de acordo com gênero, espaço social e etiologia. Materiais e Métodos: A amostra constituiu-se de 116 prontuários de pacientes reabilitados através de próteses oculares na Clínica de Prótese Buco-Maxilo-Facial da Universidade Federal de Pernambuco. A análise estatística descritiva envolveu cálculo de proporções e foram utilizados os testes estatísticos Qui-quadrado de Pearson e Exato de Fisher, quando indicados. O nível de significância estatística utilizado foi igual a 0,05.Resultados: O perfil sociodemográfico e econômico evidenciou que os pacientes eram predominantemente adultos (73,7%), do gênero masculino (69,7%), procedentes do Recife e sua Região Metropolitana (64,9%),com baixa escolaridade (79,9%) e renda individual de até dois salários mínimos (75,4%). Quanto aos fatores etiológicos, os dois principais instrumentos que provocaram traumas oculares em ambos os gêneros foram os perfurantes e os contundentes, sendo que no gênero masculino o maior percentual correspondeu aos instrumentos contundentes (42,9%). Considerando-se o espaço social onde ocorreram os traumatismos, constatou-se que eles foram mais prevalentes na rua (53,6%) e em casa (36,4%), em indivíduos do gênero masculino (70,0%). Os traumas acidentais foram os mais comuns (76,0%). Conclusão: Os danos provocados pelos traumatismos oculares estão relacionados a fatores passíveis de prevenção. Foram registradas associações significativas entrerenda e etiologia e entre os tipos de agentes etiológicos com o espaço social e a etiologia dos traumas.Descritores: Traumatismos oculares. Cegueira. Epidemiologia. Olho artificial.
Worn Out
Authors: Van Oort Madison
Published: March 2023

An immersive, first-hand account of retail worker surveillance and resistance in the digital age.

Technology has sped up the world of retail clothing, rushing affordable, trendy garments to consumers and enriching multinational retail giants such as Zara and H&M. But beneath the success of fast fashion, there is a grimmer story to be told—that of the people who do the actual producing and selling. Working undercover in two of the world's largest fast fashion stores in New York City, Madison Van Oort observed firsthand how data and surveillance shape the lives of low-status workers in an industry in flux—and how these workers are fighting back. Worn Out provides an on-the-ground look at how technology helps create this just-in-time workforce; how feminized and racialized workers experience and respond to new forms of digital control; and how collective struggles for racial, gender, and economic justice in and around retail spaces inform these workers' resistance.

Worn Out draws on interviews with dozens of front line workers and labor activists, and on evidence gathered at corporate conferences, to expose the exploitative reality of retail labor in the digital age. Van Oort shows how digital tools lubricate the shift toward just-in-time retail by collecting real-time data on not only customer behavior but also worker performance and how these tools—including automated scheduling platforms, biometric time clocks, and cashier metrics—increase these workers' already heightened insecurity. One of the first ethnographies of this “thriving” industry, Van Oort's book pulls open the curtain between production and consumption and reveals the real cost of fast fashion.
To Know Is to Compare
Authors: Matassi Mora , Boczkowski Pablo J.
Published: March 2023

How systematic comparative research can unlock the potential of social media scholarship.

Though diverse and fruitful, social media scholarship too often focuses on single platforms in single countries, disconnected from other media that people use. Mora Matassi and Pablo J. Boczkowski's alternative approach offers a framework based on the epistemological principle that everything we know emerges from comparing two or more entities. Drawing on a wealth of real-life cases, Matassi and Boczkowski examine key aspects of social media from three comparative dimensions (nations, media, and platforms) and two topics (history and language) to propose a blueprint that encourages researchers and lay readers alike to think about social media from new perspectives.

Matassi and Boczkowski illustrate their theoretical points with examples that link multiple media, illuminate an array of platforms, cover different countries and eras, and address various languages and both textual and non-textual signifiers. The result is an original conceptual account that allows for the study of social media in ways that are global, de-westernized, transmedia, and multiplatform. In addition, the authors review the major texts that use a comparative treatment and suggest topics, theories, and methods for engaging in comparative studies in the future.
The Videogame Industry Does Not Exist
Authors: Keogh Brendan
Published: March 2023

The precarious reality of videogame production beyond the corporate blockbuster studios of North America.

The videogame industry, we're invariably told, is a multibillion-dollar, high-tech business conducted by large corporations in North America, Europe, and East Asia. But, in reality, most videogames today are made by small clusters of people working on shoestring budgets, relying on existing, freely available software platforms, and hoping, often in vain, to rise to stardom—in short, people working like artists. Aiming squarely at this disconnect between perception and reality, The Videogame Industry Does Not Exist presents a more accurate and nuanced picture of how the vast majority of videogame-makers work.

Drawing on insights from over 400 game developers, Brendan Keogh develops a new framework for understanding videogame production as a cultural field in all its complexity. Part-time hobbyists, aspirational students, client-facing contractors, struggling independents, artist collectives, and tightly knit local scenes—all have a place within this model. But proponents of non-commercial game-making don't exist in isolation; Keogh shows how they and their commercial counterparts are deeply interconnected and codependent in the field of videogame production.

A cultural intervention, The Videogame Industry Does Not Exist challenges core assumptions about videogame production and reveals the diverse and precarious communities, identities, and approaches that make it a significant cultural practice.
Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans
Authors: Medwig-Kinney Taylor N , Kinney Brian A , Martinez Michael AQ , Yee Callista , Sirota Sydney S , Mullarkey Angelina A , Somineni Neha , Hippler Justin , Zhang Wan , Shen Kang , Hammell Christopher , Pani Ariel M , Matus David Q
Published: March 2023

A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. How cells switch between proliferative and invasive states is not well understood. Here, we investigated this dichotomy in vivo by examining two cell types in the developing Caenorhabditis elegans somatic gonad that derive from equipotent progenitors, but exhibit distinct cell behaviors: the post-mitotic, invasive anchor cell and the neighboring proliferative, non-invasive ventral uterine (VU) cells. We show that the fates of these cells post-specification are more plastic than previously appreciated and that levels of NHR-67 are important for discriminating between invasive and proliferative behavior. Transcription of NHR-67 is downregulated following post-translational degradation of its direct upstream regulator, HLH-2 (E/Daughterless) in VU cells. In the nuclei of VU cells, residual NHR-67 protein is compartmentalized into discrete punctae that are dynamic over the cell cycle and exhibit liquid-like properties. By screening for proteins that colocalize with NHR-67 punctae, we identified new regulators of uterine cell fate maintenance: homologs of the transcriptional co-repressor Groucho (UNC-37 and LSY-22), as well as the TCF/LEF homolog POP-1. We propose a model in which the association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo.
Inhibition of Type I PRMTs Reforms Muscle Stem Cell Identity Enhancing their Therapeutic Capacity
Authors: Dominici Claudia , Villareal Oscar , Dort Junio , Heckel Emilie , Wang Yu Chang , Ragoussis Ioannis , Joyal Jean-Sebastien , Dumont Nicolas A. , Richard Stéphane
Published: March 2023

In skeletal muscle, muscle stem cells (MuSC) are the main cells responsible for regeneration upon injury. In diseased skeletal muscle, it would be therapeutically advantageous to replace defective MuSCs, or rejuvenate them with drugs to enhance their self-renewal and ensure longterm regenerative potential. One limitation of the replacement approach has been the inability to efficiently expand MuSCs
ex vivo
, while maintaining their stemness and engraftment abilities. Herein, we show that inhibition of type I protein arginine methyltransferases (PRMTs) with MS023 increases the proliferative capacity of
ex vivo
cultured MuSCs. Single cell RNA sequencing (scRNAseq) of
ex vivo
cultured MuSCs revealed the emergence of novel subpopulations in MS023-treated cells which are defined by elevated Pax7 expression and markers of MuSC quiescence, both features of enhanced self-renewal. Furthermore, the scRNAseq identified MS023-specific subpopulations to be metabolically reprogrammed with upregulated glycolysis and oxidative phosphorylation (OxPhos). Transplantation of MuSCs treated with MS023 had a better ability to repopulate the MuSC niche and contributed more robustly to muscle regeneration following injury. Interestingly, the preclinical mouse model of Duchenne muscular dystrophy had increased bilateral grip strength 10 days after a single intraperitoneal dose of MS023. These findings suggest that type I PRMT inhibition metabolically reprograms MuSCs resulting in improved self-renewal and muscle regeneration fitness.
Expanding the stdpopsim species catalog, and lessons learned for realistic genome simulations
Authors: Lauterbur M. Elise , Cavassim Maria Izabel A. , Gladstein Ariella L. , Gower Graham , Pope Nathaniel S. , Tsambos Georgia , Adrion Jeff , Belsare Saurabh , Biddanda Arjun , Caudill Victoria , Cury Jean , Echevarria Ignacio , Haller Benjamin C. , Hasan Ahmed R. , Huang Xin , Iasi Leonardo Nicola Martin , Noskova Ekaterina , Obšteter Jana , Pavinato Vitor Antonio Corrêa , Pearson Alice , Peede David , Perez Manolo F. , Rodrigues Murillo F. , Smith Chris C. R. , Spence Jeffrey P. , Teterina Anastasia , Tittes Silas , Unneberg Per , Vazquez Juan Manuel , Waples Ryan K. , Wohns Anthony Wilder , Wong Yan , Baumdicker Franz , Cartwright Reed A. , Gorjanc Gregor , Gutenkunst Ryan N. , Kelleher Jerome , Kern Andrew D. , Ragsdale Aaron P. , Ralph Peter L. , Schrider Daniel R. , Gronau Ilan
Published: March 2023

Simulation is a key tool in population genetics for both methods development and empirical research, but producing simulations that recapitulate the main features of genomic data sets remains a major obstacle. Today, more realistic simulations are possible thanks to large increases in the quantity and quality of available genetic data, and to the sophistication of inference and simulation software. However, implementing these simulations still requires substantial time and specialized knowledge. These challenges are especially pronounced for simulating genomes for species that are not well-studied, since it is not always clear what information is required to produce simulations with a level of realism sufficient to confidently answer a given question. The community-developed framework stdpopsim seeks to lower this barrier by facilitating the simulation of complex population genetic models using up-to-date information. The initial version of stdpopsim focused on establishing this framework using six well-characterized model species (Adrion et al.,2020). Here, we report on major improvements made in the new release of stdpopsim (version 0.2), which includes a significant expansion of the species catalog and substantial additions to simulation capabilities. Features added to improve the realism of the simulated genomes include non-crossover recombination and provision of species-specific genomic annotations. Through community-driven efforts, we expanded the number of species in the catalog more than three-fold and broadened coverage across the tree of life. During the process of expanding the catalog, we have identified common sticking points and developed best practices for setting up genome-scale simulations. We describe the input data required for generating a realistic simulation, suggest good practices for obtaining the relevant information from the literature, and discuss common pitfalls and major considerations. These improvements to stdpopsim aim to further promote the use of realistic whole-genome population genetic simulations, especially in non-model organisms, making them available, transparent, and accessible to everyone.
Understanding Esports Teamplay as an Emergent Choreography
Authors: Rusk Fredrik , Ståhl Matilda , Jusslin Sofia
Published: March 2023

We, as analysts and researchers of game play, may be overlooking important aspects of players’ actions that may help us understand the interconnectedness of interactional resources, such as body, gaze, talk and avatar actions, in players’ gaming experiences. Players, from their perspective, do not necessarily concern themselves with making distinctions between, for example, off-screen and on-screen actions at all. They employ all, and whatever, interactional resources that are available to them to play together as a team. This may become especially salient in multiplayer esports games where players are geographically dispersed. This study analyses several Counter-Strike: Global Offensive (CS:GO) matches being played by esports teams, in an attempt to, from an ethnomethodological (EM) participant perspective, understand how teams coordinate, or choreograph, their game play as part of larger sequences of situationally emergent tactics. We incorporate an understanding of expanded choreography developed within the field of dance and draw on the structural possibilities of choreography, seeking to understand the actions, collaboration, and coordination in the players’ game play through analyzing interactional resources and movement qualities enacted when playing. Understanding individual players’ actions and team actions as part of a larger, emergent, choreography may help us to better realize how esports players in a team, intersubjectively, construct a ’mental map’ of current and next actions, which affect their own (individual) current and next actions.
Prevalence and risk factors of computer vision syndrome—assessed in office workers by a validated questionnaire
Authors: Cantó-Sancho Natalia , Porru Stefano , Casati Stefano , Ronda Elena , Seguí-Crespo Mar , Carta Angela
Published: March 2023

Background

Computer vision syndrome (CVS) is a common occupational health problem, but its clinical definition, prevalence and risk factors are not well defined. In general, non-validated diagnostic instruments have been used to assess its prevalence. For this reason, the aim of this study is to estimate the prevalence and potential risk factors for CVS using a validated questionnaire.

Methods

A cross-sectional study (n = 238) was carried out in Italian office workers using digital devices. All participants responded to an anamnesis, a digital exposure questionnaire, and the validated Italian version of the Computer Vision Syndrome Questionnaire. A battery of 3 ocular surface and tear ophthalmic tests (break-up time, BUT), Schirmer II and corneal staining) was performed.

Results

The mean age (±SD) was 45.55 (11.02) years, 64.3% were female. 71.4% wore glasses to work, whose design was monofocal (for distance) in 47.6%, monofocal (for near) in 26.5%, general progressive in 16.5% and occupational progressive in 8.8% of cases. 35.7% used digital devices >6 hours/day in the workplace. The prevalence of CVS was 67.2%. In the multivariate model, female sex (aOR: 3.17; 95% CI [1.75–5.73]), the use of digital devices >6 hours/day at workplace (aOR: 2.07; 95% CI [1.09–3.95]) and the use of optical correction at work (aOR: 2.69; 95% CI [1.43–5.08]) significantly increased the odds of CVS. Association was observed between presenting CVS and having abnormal BUT (χ2 = 0.017).

Conclusions

The prevalence of CVS in Italian office workers, especially among females, was high. Intensive use of digital devices at work (>6 hours/day) and the use of optical correction at work significantly increased the odds of CVS. There is an association between poor tear stability and CVS. Further research is needed on the influence of wearing optical correction on CVS. The use of a validated questionnaire in health surveillance of digital workers is strongly recommended.
MicroRNA expression in apical periodontitis and pulpal inflammation: a systematic review
Authors: Al Gashaamy Zainab Jamal , Alomar Tiba , Al-Sinjary Linah , Wazzan Mohammad , Saeed Musab Hamed , Al-Rawi Natheer H.
Published: March 2023

Background

The aim of this systematic review is to determine microRNAs (miRs) that are differently expressed between diseased pulpal and periapical tissues.

Design

This systematic review used PubMed, Scopus, EBSCO, ProQuest, Cochrane database as well as manual searching to extract studies from January 2012 up to February 2022.

Results

A total of 12 studies met the eligibility criteria were included. All selected studies were of case-control type. Twenty-four miRNAs associated with apical periodontitis, 11 were found to be upregulatedand 13 were downregulated. Four out of the 44 miRs associated with pulpal inflammation were upregulated, whereas forty were downregulated. Six miRs, namely hsa-miR-181b, hsa-miR-181c,hsa-miR-455-3p,hsa-miR-128-3p, hsa-miR199a-5p, and hsa-miR-95, exhibited considerable downregulation in both periapical and pulp tissues.

Conclusion

MiRs have been investigated for their role in pulpal and periapical biology and may be utilised in diagnostic and therapeutic purposes. Further investigations are required to determine why certain irreversible pulpitis situations progress to apical periodontitis and others do not, based on the various miR expressions. Moreover, clinical and laboratory trials are needed to support this theory.
CYP27A1 deficiency promoted osteoclast differentiation
Authors: Fang Ziqi , Cheng Guangdong , He Mengting , Lin Yanliang
Published: March 2023

Background

The elevating osteoclast differentiation can lead to an imbalance in bone homeostasis, which was responsible for bone loss and bone diseases, such as osteoporosis. Multiple pathways and molecules have been involved in osteoclast formation, but the role of CYP27A1 in osteoclast differentiation has never been explored.

Methods

CYP27A1 deficient mice were constructed using CRISPR-Cas9 system. Osteoclast differentiation was detected by TRAP staining. Differentially expressed genes (DEGs) were identified using RNA-seq analysis and were confirmed by qRT-PCR and Western blot.

Results

The results showed that CYP27A1 knockout (KO) promoted osteoclast differentiation and bone loss. The transcriptomic analysis revealed that CYP27A1 KO led to differential expression of multiple genes, including ELANE, LY6C2, S100A9, GM20708, BGN, SPARC, and COL1A2, which were confirmed by qRT-PCR and Western blot. Enrichment analysis indicated that these differential genes were significantly associated with osteogenesis-related pathways, such as PPAR signaling, IL-17 signaling, and PI3K/AKT signaling, which were confirmed by qRT-PCR and Western blot.

Conclusions

These results suggested that CYP27A1 was involved in osteoclast differentiation, providing a novel therapeutic target for osteoclast-related diseases.
Effects of an Interactive Smoking Cessation Coaching Program on Smoking in Female College Students
Authors: Kwon Youn-Suk , Choi Eun-Suk
Published: March 2023

Purpose: This study aimed to examine the effects of an interactive smoking cessation coaching program on smoking cessation motivation, smoking knowledge, smoking shame, urine cotinine levels, tobacco craving and smoking abstinence self-efficacy. The conceptual framework of the study applied Cox’s interaction model of client health behavior for female college students who smoked.Methods: This was a before-and-after experimental design study with a non-equivalent control group. The participants were smoking female college students, who were assigned either to an experimental group (n=22) or a control group (n=24). The interaction model of client health behavior was the theoretical basis. The core tactic of smart management and strategy for health was applied as a coaching technique. The experimental group attended 11 sessions of an interactive smoking cessation coaching program, (60 to 120 minutes per session) for 6 weeks, and the control group received education regarding smoking cessation after the program finished. The data collection period was from January 23, 2019 to March 7, 2019.Results: This study showed differences in smoking cessation motivation (F=71.09, p<.001), smoking knowledge (F=20.77, p<.001), smoking shame (t=5.11, p<.001), urine cotinine levels (t=-9.58, p<.001) and smoking abstinence self-efficacy (t=11.68, p<.001). However, no difference in tobacco cravings (t=-1.57, p=.127) was found.Conclusion: As a result of the interactive smoking cessation coaching program, statistically significant differences were found in smoking cessation motivation, smoking knowledge, smoking shame, urine cotinine levels and smoking abstinence self-efficacy. However, further research is needed because there was no statistically significant difference in tobacco cravings.
Factors Influencing Senior Nursing Students’ COVID-19 Psychological Distress: A Cross-sectional Study
Authors: Lee Kang Sun , Kim Hye Young
Published: March 2023

Purpose: The purpose of this study was to examine the relationship between coronavirus disease 2019 (COVID-19)-related knowledge, preventive behaviors, risk perception, nursing professional values, and COVID-19 psychological distress among nursing students, and to identify factors associated with senior nursing students’ COVID-19 psychological distress.Methods: This descriptive cross-sectional study included fourth-year nursing students (n=183). Data were collected using a structured questionnaire between May and June 2022. Data were analyzed using descriptive statistics, the independent t-test, one-way analysis of variance, the Scheffé test, Pearson’s correlation coefficients, and multiple linear regression analysis, using SPSS version 27.0 for Windows.Results: COVID-19 risk perception (β=.39, p<.001) had the strongest association with COVID-19-related psychological distress, followed by COVID-19 knowledge (β=.37, p<.001), major satisfaction (β=-.19, p=.004), and COVID-19 preventive behavior (β=-.16, p=.023). These variables accounted for approximately 27% of the variance in COVID-19-related psychological distress.Conclusion: Nursing educators should work to develop interventions to reduce nursing students’ COVID-19-related psychological distress and should consider COVID-19 knowledge, COVID-19 risk perception, and COVID-19 preventive behavior. Furthermore, plans should be developed to enhance students’ satisfaction with the nursing major by improving the clinical practicum suited to their needs.
Influence of Fall-related Perception on Behavioral Intention of Fall Prevention among Hospitalized Older Patients
Authors: Kim Young Ju , Kim Su Hyun
Published: March 2023

Purpose: This study aimed 1) to identify hospitalized older patients' fall-related perceptions (including subjective fall risk assessment, confidence in performing fall risk behavior, fear of falling, and recognition of fall consequences) and behavioral intention for fall prevention, and 2) to investigate the influence of fall-related perceptions on the behavioral intention for fall prevention.Methods: A cross-sectional survey was conducted among 150 hospitalized older patients admitted to a large general hospital. A structured questionnaire was administered from October 1, 2020 to January 31, 2021. Cohen's kappa and multiple linear regression analysis were utilized to analyze the data.Results: The average scores were 1.43±0.65 for the subjective fall risk assessment, 4.08±0.44 for confidence in performing fall risk behavior, and 2.04±0.61 for the behavioral intention for fall prevention, on a 1~5 scale, with higher scores indicating higher levels of the attributes. They had an average of 1.48±0.56 for fear of falling and 2.43±0.35 for the recognition of fall consequences, on a 1~4 scale. Low agreement was found between the subjective fall risk assessment by hospitalized elderly patients and the objective fall risk assessment by nurses using the Morse Fall Scale. The hospitalized elderly patients' subjective fall risk assessment, confidence in performing fall risk behavior, and fear of falling were significant factors influencing behavioral intention for fall prevention.Conclusion: The approach to preventing falls among hospitalized older patients should include assessing fall-related perceptions and providing education to correct inappropriate perceptions about falls.
Structures Surrounding the ‘User’ in User Engagement: Gender-based Violence Design Engagements
Authors:   Royal College of Art, Fiadeiro Rute , Stevens John ,   Royal College of Art, Bichard Jo-Anne ,   Royal College of Art
Published: March 2023

User engagement is a dynamic social process influenced by who is involved and how. Here we argue that designers must account for the structural conditions of users’ lives, as they may have safety, accountability, and political implications. We review current scholarship in the area of user configuration and engagement from a ‘structural’ viewpoint of gender-based violence (GBV), to better understand such considerations. We propose three dimensions that might support designers in deepening their engagement in this area, namely: construction of the user, engagement within the context, and the designers’ position. We combine these dimensions as a framework to review and compare examples of designed outcomes for GBV prevention. This article suggests thoughts and questions to be considered by designers for thinking more structurally about GBV design, and for other contexts involving people experiencing vulnerability.
Design In and From the Periphery: Building a Praxis of Resistance through Collective Investigations
Authors:   Universidade Federal do Pará, Batista e Silva Sâmia
Published: March 2023

This article presents notes resulting from qualitative research with a participatory approach, carried out in person and remotely with a group of young people from the Terra Firme neighborhood, in Belém-Pará, northern Brazil. The aim was to analyze how the engagement of designers in emancipation processes managed by socially oppressed groups can promote transformations in the practices of designers in participatory projects. The theoretical foundation is based on the Latin American critical thinking of authors such as Paulo Freire and Orlando Fals Borda, demonstrating how their legacy influenced designers in participatory projects. The theoretical framework and qualitative research allowed us to consider that the engagement of designers in popular struggles not only influences the change in the scope of projects, but also allows solidarity to emerge as their main element