bluuebunny/crossref_metadata_2025 huggingface dataset:
Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study
Authors: Pan Shin-Chen , Lai Chi-Hung , Vu Van-Truc , Vu Cao-An , Huang Chun-Jen , Cheng Chao-Min , Chen Wen-Yih
Published: June 2023

Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, the role of exosomal miR-21 in wounds has yet to be well studied. In order to facilitate the early management of poorly healing wounds, we developed an easy-to-use, rapid, paper-based microfluidic-exosomal miR-21 extraction device to determine wound prognosis in a timely manner. We isolated and then quantitatively examined exosomal miR-21 in wound fluids from normal tissues and acute and chronic wounds. Eight improving wounds displayed lower levels of exosomal miR-21 expression after wound debridement. However, four instances of increased exosomal miR-21 expression levels were notably associated with patients with poor healing wounds despite aggressive wound debridement, indicating a predictive role of tissue exosomal miR-21 for wound outcome. Paper-based nucleic acid extraction device provides a rapid and user-friendly approach for evaluating exosomal miR-21 in wound fluids as a means of monitoring wounds. Our data suggest that tissue exosomal miR-21 is a reliable marker for determining current wound status.
Features of DNA–Montmorillonite Binding Visualized by Atomic Force Microscopy
Authors: Kraevsky Sergey V. , Barinov Nikolay A. , Morozova Olga V. , Palyulin Vladimir V. , Kremleva Alena V. , Klinov Dmitry V.
Published: June 2023

In the present work, complexes of DNA with nano-clay montmorillonite (Mt) were investigated by means of atomic force microscopy (AFM) under various conditions. In contrast to the integral methods of analysis of the sorption of DNA on clay, AFM allowed us to study this process at the molecular level in detail. DNA molecules in the deionized water were shown to form a 2D fiber network weakly bound to both Mt and mica. The binding sites are mostly along Mt edges. The addition of Mg2+ cations led to the separation of DNA fibers into separate molecules, which bound mainly to the edge joints of the Mt particles according to our reactivity estimations. After the incubation of DNA with Mg2+, the DNA fibers were capable of wrapping around the Mt particles and were weakly bound to the Mt edge surfaces. The reversible sorption of nucleic acids onto the Mt surface allows it to be used for both RNA and DNA isolation for further reverse transcription and polymerase chain reaction (PCR). Our results show that the strongest binding sites for DNA are the edge joints of Mt particles.
A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace
Authors: Santana-Sosa Silvia , Matos-Perdomo Emiliano , Ayra-Plasencia Jessel , Machín Félix
Published: June 2023

The morphology of the nucleus is roughly spherical in most eukaryotic cells. However, this organelle shape needs to change as the cell travels through narrow intercellular spaces during cell migration and during cell division in organisms that undergo closed mitosis, i.e., without dismantling the nuclear envelope, such as yeast. In addition, the nuclear morphology is often modified under stress and in pathological conditions, being a hallmark of cancer and senescent cells. Thus, understanding nuclear morphological dynamics is of uttermost importance, as pathways and proteins involved in nuclear shaping can be targeted in anticancer, antiaging, and antifungal therapies. Here, we review how and why the nuclear shape changes during mitotic blocks in yeast, introducing novel data that associate these changes with both the nucleolus and the vacuole. Altogether, these findings suggest a close relationship between the nucleolar domain of the nucleus and the autophagic organelle, which we also discuss here. Encouragingly, recent evidence in tumor cell lines has linked aberrant nuclear morphology to defects in lysosomal function.
Thermally Stable and Reusable Silica and Nano-Fructosome Encapsulated CalB Enzyme Particles for Rapid Enzymatic Hydrolysis and Acylation
Authors: Jang Woo Young , Sohn Jung Hoon , Chang Jeong Ho
Published: June 2023

This study reports the preparation of silica-coated and nano-fructosome encapsulated Candida antarctica lipase B particles (CalB@NF@SiO2) and a demonstration of their enzymatic hydrolysis and acylation. CalB@NF@SiO2 particles were prepared as a function of TEOS concentration (3–100 mM). Their mean particle size was 185 nm by TEM. Enzymatic hydrolysis was performed to compare catalytic efficiencies of CalB@NF and CalB@NF@SiO2. The catalytic constants (Km, Vmax, and Kcat) of CalB@NF and CalB@NF@SiO2 were calculated using the Michaelis–Menten equation and Lineweaver–Burk plot. Optimal stability of CalB@NF@SiO2 was found at pH 8 and a temperature of 35 °C. Moreover, CalB@NF@SiO2 particles were reused for seven cycles to evaluate their reusability. In addition, enzymatic synthesis of benzyl benzoate was demonstrated via an acylation reaction with benzoic anhydride. The efficiency of CalB@NF@SiO2 for converting benzoic anhydride to benzyl benzoate by the acylation reaction was 97%, indicating that benzoic anhydride was almost completely converted to benzyl benzoate. Consequently, CalB@NF@SiO2 particles are better than CalB@NF particles for enzymatic synthesis. In addition, they are reusable with high stability at optimal pH and temperature.
Building Information Modelling for Application in Geotechnical Engineering
Authors: Satyanaga Alfrendo , Aventian Gerarldo Davin , Makenova Yerkezhan , Zhakiyeva Aigerim , Kamaliyeva Zhuldyz , Moon Sung-Woo , Kim Jong
Published: June 2023

BIM (Building Information Modelling) is used to create and manage data during design, construction, and operation. It helps to effectively manage resources and optimize processes in the construction industry. Geotechnical engineering is one of the complex disciplines that may require BIM integration. Various data types must be provided in a timely manner and require real-time feedback, fast processing, and construction guidance. The first problem presented in the paper is the use of the traditional 2D-based method used by engineers for a particular task. It seems to be impractical when some adjustments are included. Another issue is the lack of communication between the workers. It poses the problem of information exchange and misunderstanding during the interpretation of technical data. This paper aims to find different integration techniques and steps for integrating geotechnical data into the BIM process. Methods used to examine the topic are qualitative research, literature review, and case studies. These methods were useful for studying the problems and introducing the soil information into the BIM application. Firstly, a case study with I-BIM was considered, and the BIM–FEM–BIM interaction was applied to introduce geotechnical information with Plaxis 3D. The results have shown that further development of BIM in infrastructure is needed. Another case study explored the present state of the geotechnical design in BIM and potential solutions. The new frameworks were recreated: many boreholes were imported to the BIM, and a 3D geometric model of the entire hill was created for the hill fortification structure with soil clogging. The last two studies in Malaysia modeled a 3D subsurface and used two geotechnical formats, AGS and CVS. The first includes more information than the second; however, the second can be used for a more generalized model. Overall, BIM–FEM interaction can be used as a geometric model for data transfer. However, the open data format of the Industry Foundation Class (IFC) or geotechnical data format of the AGS and CVS were suggested to be used for greater flexibility. It was also found that excessive information makes the model loaded and complex. Therefore, it was recommended that big data be summarized properly with minimal loss of necessary data. Further research is needed to understand data transmission schemes of geotechnical information better. Moreover, it is recommended to put all the strategies directly into practice to create a geotechnical design.
Beyond the Gut: A Systematic Review of Oral Manifestations in Celiac Disease
Authors: Lucchese Alberta , Di Stasio Dario , De Stefano Simona , Nardone Michele , Carinci Francesco
Published: June 2023

Background: Celiac disease (CD) is a chronic immune-mediated gluten-sensitive enteropathy, affecting about 1% of the population. The most common symptoms include diarrhea, abdominal pain, weight loss, and malabsorption. Extra-intestinal symptoms include oral manifestations. This systematic review aims to catalog and characterize oral manifestations in patients with CD. Methods: a systematic literature review among different search engines using PICOS criteria has been performed. The studies included used the following criteria: tissues and anatomical structures of the oral cavity in humans, published in English and available in full text. Review articles and papers published before 1990 were excluded. Results: 209 articles were identified in the initial search. In the end, 33 articles met the selection criteria. The information extracted from the articles was classified based on the type of oral manifestation. Recurrent aphthous stomatitis (34.6%), atrophic glossitis and geographic tongue (15.26%), enamel defects (42.47%), delayed dental eruption (47.34%), xerostomia (38.05%), glossodynia (14.38%), and other manifestations including cheilitis, fissured tongue, periodontal diseases, and oral lichen planus were found in the celiac subjects of the studies analyzed. The quality of articles on the topic should be improved; however, oral manifestations in CD patients are widely described in the literature and could help diagnose celiac disease.
Visual and Anatomical Outcomes of a Single Intravitreal Dexamethasone in Diabetic Macular Edema: An 8 Year Real-World Study
Authors: Faes Livia , Mishra Amit V. , Lipkova Veronika , Balaskas Konstantinos , Quek Chrystie , Hamilton Robin , Held Ulrike , Sim Dawn , Sivaprasad Sobha , Fu Dun Jack
Published: June 2023

Importance: Diabetic macular edema (DME) is a major cause of vision loss in patients with diabetes mellitus. Intravitreal dexamethasone is a treatment option for patients unsuitable for or non-responsive to anti-angiogenic agents. Objective: To quantify visual and anatomical outcomes from an initial intravitreal dexamethasone injection over the expected 6-month period of dexamethasone release by the implant. Design and enrolment: This is a retrospective cohort study using electronic medical records of patients reviewed between 1 January 2012 and 1 April 2022. Setting: A tertiary eye-care center in London, United Kingdom; Moorfields Eye Hospital National Healthcare System Foundation Trust. Participants: The cohort comprised 418 adult patients with DME who received an initial treatment of 700 µg intravitreal dexamethasone in the study period. Of these, 240 patients met the inclusion criteria of ≥2 hospital visits following initial injection (≥1 beyond 6 months) and no previous ocular corticosteroid treatment or missing assessment at baseline. Exposure(s): Intravitreal dexamethasone implant (700 µg). Main Outcome(s) and Measure(s): Probability of a positive visual outcome, defined as ≥5 or ≥10 Early Treatment Diabetic Retinopathy Study (ETDRS)-letter gain after treatment when compared to baseline (Kaplan–Meier models). Results: From the initial intravitreal dexamethasone injection alone, we observed a >75% chance of gaining ≥5 ETDRS letters and >50% chance of gaining ≥10 ETDRS letters within 6 months. There was less than a 50% chance of sustaining either positive visual outcome beyond 4 months. Conclusions and Relevance: Most patients can be expected to have a positive visual outcome following an initial injection of dexamethasone implants that subsides within 4 months. Real-world re-treatment was observed to be delayed until after visual benefits were lost in half of the cohort. Further research will be needed to study the effects of delays in re-treatment.
Treatment and Prevention of Postoperative Leakage after Gastrectomy for Gastric Cancer
Authors: Jeong Sang-Ho , Lee Jin-Kwon , Seo Kyung Won , Min Jae-Seok
Published: June 2023

Anastomotic leakage is one of the common causes of serious morbidity and death after gastrectomy. The use of surgical treatment for leakage decreased due to the development of nonsurgical management. However, if nonsurgical management fails to control the spread of intra-abdominal infection, emergency surgical treatment is required. The authors wished to determine in which cases surgical treatment is needed for postoperative leakage and to identify treatment and prevention strategies. If a patient’s vital signs are stable, local abscesses can be cured by conservative treatment after percutaneous drain insertion; if there is no improvement in anastomotic leakage, endoscopic treatment such as clipping, vacuum, and stent placement can be performed. If a patient’s vital signs are unstable or patient shows diffuse peritonitis, surgical treatment should be performed. A surgical plan can be established according to leakage location. The duodenal stump may first require conservative treatment. It is recommended that surgical treatment be attempted first for anastomotic leakage of gastrojejunostomy site and gastric stump in remnant stomach. In conclusion, the need for surgical treatment is determined depending on vital signs and presence of diffuse peritonitis. During surgical treatment, a strategic approach is required according to the patient’s condition and the anatomical location of leakage.
Impact of Routinely Performed Optical Coherence Tomography Examinations on Quality of Life in Patients with Retinal Diseases—Results from the ALBATROS Data Collection
Authors: Schuster Alexander K. , Wolfram Christian , Hudde Tobias , Klatt Alexander , Schnegelsberg Birthe , Midani-Oezkan Heven , Ross Mike , Ziemssen Focke , Pfeiffer Norbert
Published: June 2023

The use of OCT to monitor intravitreal treatment varies in clinical practice and is not always mandatory. The ALBATROS data collection aimed to clarify the impact of routinely implemented OCT on clinical outcomes and its impact on vision-related quality of life (VRQoL). Methods: An observational cohort study included patients with retinal diseases starting an intravitreal anti-vascular endothelial growth factor treatment in Germany. Treatment followed clinical practice except mandatory OCT examination during the 12-month observation period. VRQoL was assessed by NEI VFQ-25 and compared with respect to OCT examinations and number of intravitreal injections in the different diseases (nAMD, DME, BRVO, CRVO). Results: 1478 patients (74.5 ± 10.9 years, 54.9% female) were included in the analysis. Patients had neovascular AMD (65.2%), DME (18.4%), BRVO (9.5%), or CRVO (6.9%). 8.8 ± 2.6 OCT examinations and 6.1 ± 3.2 intravitreal injections were performed within 12 months. VRQoL differed between indications at baseline, with substantially lower values for neovascular AMD and CRVO. After twelve months, an increase in visual acuity and visual functional scale was observed for nAMD, DME, and BRVO, while in DME only, there was an association between number of OCT examinations and VRQoL. Conclusion: Intravitreal treatment was able to maintain VRQoL over twelve months in a real-world setting. Regular OCT examinations were associated with higher gain in VRQoL in DME patients after 12 months.
Red Wine and Sexual Function in Men: An Original Point of View
Authors: Basile Livia , Condorelli Rosita , Calogero Aldo , Cannarella Rossella , Barbagallo Federica , Crafa Andrea , Aversa Antonio , La Vignera Sandro
Published: June 2023

Red wine is a rich source of nutrients whose biological properties have inspired numerous scientific studies. Indeed, it has been widely reported that there is a correlation between the positive health effects of moderate consumption of red wine and its phenolic content, which, due to its antioxidant activity, has proved to be useful in the improvement of various diseases, such as cardiovascular diseases, metabolic syndrome, cognitive disorders, depression, and cancer. It is a common opinion that the antioxidant activity of red wine is to be ascribed to its entire content of polyphenols, which act synergistically and not as a single component. Furthermore, this health-promoting effect of red wine can also be linked to its ethanol content, which has shown a wide array of biological properties. Beyond this evidence, very little is known about a possible correlation between moderate consumption of red wine and male sexual function. This brief review aimed to evaluate the effects of moderate consumption of red wine on erectile function. To accomplish this, Pubmed and Google Scholar databases were searched to retrieve the most relevant studies on this topic. The evidence so far collected has shown that red wine, if consumed in moderation, can be potentially beneficial for patients with erectile dysfunction as well as can positively influence reproductive function through mechanisms that depend on the vasorelaxant properties of red wine and its antioxidant properties.
Predicting Preterm Birth with Strain Ratio Analysis of the Internal Cervical Os: A Prospective Study
Authors: Luca Alina-Madalina , Haba Raluca , Cobzeanu Luiza-Maria , Nemescu Dragos , Harabor Anamaria , Mogos Raluca , Adam Ana-Maria , Harabor Valeriu , Nechita Aurel , Adam Gigi , Carauleanu Alexandru , Scripcariu Sadiye-Ioana , Vasilache Ingrid-Andrada , Gisca Tudor , Socolov Demetra
Published: June 2023

(1) Background: Cervical elastography is a new concept that could allow clinicians to assess cervical consistency in various clinical scenarios. We aimed to evaluate the predictive performance of the strain ratio (SR) at the level of the internal os, either individually or in combination with other parameters, in the prediction of spontaneous preterm birth (PTB) at various gestational ages. (2) Methods: This prospective study included 114 pregnant patients with a high-risk profile for PTB who underwent cervical elastography during the second trimester. Clinical and paraclinical data were assessed using univariate analysis, logistic regression, and sensitivity analysis. (3) Results: The SR achieved an area under the receiver operating curve (AUROC) value of 0.850, a sensitivity of 85.71%, and a specificity of 84.31% in the prediction of PTB before 37 weeks of gestation. The combined model showed superior results in terms of accuracy (AUROC = 0.938), sensitivity (92.31%), and specificity (95.16%). When considering PTB subtypes, the highest AUROC value (0.80) and accuracy (95.61%) of this marker were achieved in the prediction of extremely preterm birth, before 28 weeks of gestation. (4) Conclusions: The SR achieved an overall good predictive performance in the prediction of PTB and could be further evaluated in various cohorts of patients.
Impact of Clinical Pharmacist Running Anticoagulation Clinic in Saudi Arabia
Authors: Alshaiban Abdulrahman , Alavudeen Sirajudeen S. , Alshahrani Ibrahim , Kardam Abdulaziz M. , Alhasan Ibrahim Mohammed , Alasiri Saleh Abdulrahman , Imam Mohammad Tarique , Almalki Ziyad Saeed , Akhtar Md Sayeed
Published: June 2023

Despite the effectiveness of warfarin in extended anticoagulation, its narrow therapeutic index requires frequent dose adjustments and careful patient monitoring. Thus, we aimed to evaluate the outcomes of clinical pharmacists’ intervention in warfarin therapy management in terms of International Normalized Ratio (INR) control, reduction of bleeding, and hospitalization in a tertiary care hospital. An observational retrospective cohort study was conducted on 96 patients taking warfarin therapy in a clinical pharmacist-led anticoagulation clinic. We observed that 39.6% of patients required dose adjustments at their first and second visits. However, dose adjustments during the third, fourth, and fifth weeks were required at 31.1%, 20.8%, and 4.2%, respectively, to achieve INR levels. We also observed that 36.46% of the patients attained the target INR at baseline, which was increased over the first week to the fifth week to 57.29%, 61.46%, 61.46%, 68.75%, and 85.42%, respectively. No one reported the ADR between the third and fifth weeks. Based on our findings, the study strongly suggests that pharmacists’ interventions can improve the health-related quality of life of patients undergoing warfarin therapy. Thus, competent pharmacy personnel must be a priority in both usual patient care and critical care among primary care networks.
Cellular and Molecular Players in the Tumor Microenvironment of Renal Cell Carcinoma
Authors: Lasorsa Francesco , Rutigliano Monica , Milella Martina , Ferro Matteo , Pandolfo Savio Domenico , Crocetto Felice , Tataru Octavian Sabin , Autorino Riccardo , Battaglia Michele , Ditonno Pasquale , Lucarelli Giuseppe
Published: June 2023

Globally, clear-cell renal cell carcinoma (ccRCC) represents the most prevalent type of kidney cancer. Surgery plays a key role in the treatment of this cancer, although one third of patients are diagnosed with metastatic ccRCC and about 25% of patients will develop a recurrence after nephrectomy with curative intent. Molecular-target-based agents, such as tyrosine kinase inhibitors (TKIs) and immune checkpoint inhibitors (ICIs), are recommended for advanced cancers. In addition to cancer cells, the tumor microenvironment (TME) includes non-malignant cell types embedded in an altered extracellular matrix (ECM). The evidence confirms that interactions among cancer cells and TME elements exist and are thought to play crucial roles in the development of cancer, making them promising therapeutic targets. In the TME, an unfavorable pH, waste product accumulation, and competition for nutrients between cancer and immune cells may be regarded as further possible mechanisms of immune escape. To enhance immunotherapies and reduce resistance, it is crucial first to understand how the immune cells work and interact with cancer and other cancer-associated cells in such a complex tumor microenvironment.
Platelets Rich Plasma (PRP) Procedure in the Healing of Atonic Wounds
Authors: Horgos Maur Sebastian , Pop Ovidiu Laurean , Sandor Mircea , Borza Ioan Lucian , Negrean Rodica Anamaria , Cote Adrian , Neamtu Andreea-Adriana , Grierosu Carmen , Sachelarie Liliana , Huniadi Anca
Published: June 2023

(1) Background: Patients suffering from chronic wounds report physical, mental, and social consequences due to their existence and care. There is a global need for tissue repair strategies and, in our case, for chronic wound healing. PRP therapy is based on the fact that platelet-derived growth factors (PGF) support the three phases of the wound healing and repair cascade (inflammation, proliferation, and remodeling); (2) Methods: A comparative study was carried out on two groups of patients with atonic wounds totaling a total of 80 cases as follows: a study group in which the PRP procedure was applied and a control group in which the biological product was not injected. The study was carried out in the surgery clinic of the Clinical Hospital C.F. Oradea City; (3) Results: A much faster healing was achieved in the case of patients who benefited from the platelet-rich plasma injection therapy compared to the group of patients in whom this therapy was not used. Three weeks after the plasma injection, a considerable reduction of the wound was evident, with some of the patients presenting with a closed wound; (4) Conclusions: The effect of PRP on the healing of chronic wounds is promising in most cases. A positive effect was also highlighted in terms of reducing treatment costs by considerably reducing the materials used as well as the number of hospitalizations for the same pathology.
PGS/Gelatin Nanocomposite Electrospun Wound Dressing
Authors: Naseri Mahyar , Hedayatnazari Aysan , Tayebi Lobat
Published: June 2023

Infectious diabetic wounds can result in severe injuries or even death. Biocompatible wound dressings offer one of the best ways to treat these wounds, but creating a dressing with a suitable hydrophilicity and biodegradation rate can be challenging. To address this issue, we used the electrospinning method to create a wound dressing composed of poly(glycerol sebacate) (PGS) and gelatin (Gel). We dissolved the PGS and Gel in acetic acid (75 v/v%) and added EDC/NHS solution as a crosslinking agent. Our measurements revealed that the scaffolds’ fiber diameter ranged from 180.2 to 370.6 nm, and all the scaffolds had porosity percentages above 70%, making them suitable for wound healing applications. Additionally, we observed a significant decrease (p < 0.05) in the contact angle from 110.8° ± 4.3° for PGS to 54.9° ± 2.1° for PGS/Gel scaffolds, indicating an improvement in hydrophilicity of the blend scaffold. Furthermore, our cell viability evaluations demonstrated a significant increase (p < 0.05) in cultured cell growth and proliferation on the scaffolds during the culture time. Our findings suggest that the PGS/Gel scaffold has potential for wound healing applications.
Prevalence of Sarcopenia in Older Patients in Rehabilitation Wards
Authors: Brugliera Luigia , Giordani Alessandra , D’Angelo Giuseppe , Trimarchi Caterina , Villa Giulia , Yen Tao-Yu , Bosica Francesco , Malatino Lorenzo , Zweiker David , Negro Alessandra , Alemanno Federica , Iannaccone Sandro
Published: June 2023

The multidisciplinary assessment of hospitalized patients via validated scales and tools has become crucial in the early identification of sarcopenia. The objective of this study was to determine the prevalence of sarcopenia and its related factors in patients aged ≥65 years admitted to the neurological rehabilitation departments of cognitive motor disorders and functional motor rehabilitation at the IRCCS Hospital San Raffaele in Milan. Using the algorithm reported by the European Working Group on Sarcopenia in Older People (EWGSOP2), the prevalence of sarcopenia in patients was investigated from 2019–2020. Definite sarcopenia was detected in 161 of 336 recruited patients (47.9%). Age was significantly higher in sarcopenic patients than in those without sarcopenia (median 81 vs. 79 years, p < 0.001) and height, weight, and body mass index were lower (p < 0.001 for all). The malnutrition screening test (MUST) was higher but still negative in most sarcopenic patients (47.8% vs. 20.6%, p < 0.001). Patients with sarcopenia had significantly reduced life autonomy (by Barthel index, median 55 vs. 60 points, p < 0.001) and increased mental impairment (tested by MMSE and MOCA, p < 0.005 for both). In conclusion, sarcopenic patients were more cognitively impaired and less autonomous in their daily life, but the majority presented with a negative malnutrition screening test.
Perception of Pathologists in Poland of Artificial Intelligence and Machine Learning in Medical Diagnosis—A Cross-Sectional Study
Authors: Ahmed Alhassan , Brychcy Agnieszka , Abouzid Mohamed , Witt Martin , Kaczmarek Elżbieta
Published: June 2023

Background: In the past vicennium, several artificial intelligence (AI) and machine learning (ML) models have been developed to assist in medical diagnosis, decision making, and design of treatment protocols. The number of active pathologists in Poland is low, prolonging tumor patients’ diagnosis and treatment journey. Hence, applying AI and ML may aid in this process. Therefore, our study aims to investigate the knowledge of using AI and ML methods in the clinical field in pathologists in Poland. To our knowledge, no similar study has been conducted. Methods: We conducted a cross-sectional study targeting pathologists in Poland from June to July 2022. The questionnaire included self-reported information on AI or ML knowledge, experience, specialization, personal thoughts, and level of agreement with different aspects of AI and ML in medical diagnosis. Data were analyzed using IBM® SPSS® Statistics v.26, PQStat Software v.1.8.2.238, and RStudio Build 351. Results: Overall, 68 pathologists in Poland participated in our study. Their average age and years of experience were 38.92 ± 8.88 and 12.78 ± 9.48 years, respectively. Approximately 42% used AI or ML methods, which showed a significant difference in the knowledge gap between those who never used it (OR = 17.9, 95% CI = 3.57–89.79, p < 0.001). Additionally, users of AI had higher odds of reporting satisfaction with the speed of AI in the medical diagnosis process (OR = 4.66, 95% CI = 1.05–20.78, p = 0.043). Finally, significant differences (p = 0.003) were observed in determining the liability for legal issues used by AI and ML methods. Conclusion: Most pathologists in this study did not use AI or ML models, highlighting the importance of increasing awareness and educational programs regarding applying AI and ML in medical diagnosis.
A Study on the Spatial and Temporal Variation of Summer Surface Temperature in the Bosten Lake Basin and Its Influencing Factors
Authors: Jumai Miyesier , Kasimu Alimujiang , Liang Hongwu , Tang Lina , Aizizi Yimuranzi , Zhang Xueling
Published: June 2023

The land surface temperature (LST) is an important indicator reflecting the ecological environment condition. As a sensitive area to climate change, mastering the spatial and temporal changes of summer LST in the Bosten Lake basin (BLB) helps gain insight into the evolution of the thermal environment in the Bosten Lake basin and for long-term monitoring of the basic ecological changes in the basin. Based on MOD11A1 data from 2005 to 2020, this paper investigates the diurnal LST spatiotemporal series variation and its influencing factors in the Bosten Lake basin by using surface temperature class classification, trending analysis, the Hurst index, and geographic probes. The results show that (1) the wetland grasslands in and around the Bayinbruck steppe in the northwestern part of the study area exhibit a heat island effect during the day, while the opposite is true at night. In terms of temporal changes, LST changes in the BLB fluctuate widely, having a general rising and then decreasing trend. (2) The decreasing trend of LST from 2005 to 2020 is significant during the daytime and vice versa at night, and the change at night is greater than during the day. The areas with significantly higher diurnal LST in the future have all expanded compared to the area occupied by them now, with an overall trend of a steady increase. (3) The dominant factor of LST variation has the strongest explanatory power when altitude and NDVI are combined during the daytime and the strongest explanatory power when NPP and temperature are combined at night.
Beyond Biodiversity: Eliciting Diverse Values of Urban Green Spaces in Flanders
Authors: Bastiaensen Thomas , Van Wambeke Ewaut , El Bakkali Camelia , Desair Jomme , Noël Charlotte , Kenis Kaat , Vincke Lukas , Jacobs Sander
Published: June 2023

Nature-based solutions are claimed to offer an effective approach to tackle societal challenges and promote biodiversity. While research has mainly focused on biodiversity and material ecosystem services, non-material contributions and relational values of urban green spaces remain underexplored. How to balance the benefits of nature, well-being, and relational values in their design and performance evaluation remains unclear. To elicit the values expressed in public communication regarding the benefits of urban nature projects in Flanders, three online repositories that feature diverse nature-based solutions projects in the region were chosen. Using coding and quantitative content analysis of standardized descriptions from these repositories, this study found that relational values were most abundant (55%), followed by instrumental values (30%) and intrinsic values (15%), consistently so over socio-demographic and physical contexts. It was also discovered that larger projects have a higher level of multifunctionality, which is calculated based on the variety of values and value dimensions expressed, and that participation—although considered key—rarely reports on inclusivity. The findings suggest that in Flanders, a greater emphasis is placed on relational values associated with urban nature. A broader value scope for the design, management, and evaluation of urban green spaces tailored to the local context is recommended.
The Communication Patterns between Speech–Language Therapists and Parents/Guardians of Children with Developmental Language Disorders in Private Speech–Language Therapy in Cyprus
Authors: Voniati Louiza , Armostis Spyros , Kilili-Lesta Margarita , Tafiadis Dionysios , Giannakou Konstantinos
Published: June 2023

Children with developmental language disorder (DLD) experience communication difficulties and receive speech–language therapy (SLT) services in public schools and/or private clinics in Cyprus. This study aims to analyze the context and content of communication between parents/guardians (P/Gs) and speech-language therapists (SLTs) in private practice. The conducted cross-sectional study utilized nonprobability convenience sampling to recruit P/Gs of children with DLD. Participants completed a questionnaire regarding their sociodemographic characteristics, the context of communication, topics discussed, and collaboration level with the SLTs. A total of 189 P/Gs participated, of which 84.1% were married, 79.4% were mothers, and 52.4% held at least a college/university degree. Mothers primarily communicated with SLTs in the setting, discussing their children’s performance, progress, behavior, difficulties, and homework. However, 48.7% felt well-informed, 47.1% fully understood their children’s goals, 55.6% received thorough briefings from SLTs, 51.3% observed at least a portion of the therapy session, and 77.8% received at least an adequate amount of homework. Additionally, 73.6% reported the use of technology during SLT, and 74.5% expressed good/great satisfaction with their children’s progress. Of the 31.1% reporting difficulties, 64.3% mentioned some difficulties related to homework completion and the children’s behavior. The proposed solutions included increased SLT briefings, training, and P/Gs observing therapy sessions.
RET Proto-Oncogene Variants in Patients with Medullary Thyroid Carcinoma from the Mediterranean Basin: A Brief Report
Authors: Neocleous Vassos , Fanis Pavlos , Frangos Savvas , Skordis Nicos , Phylactou Leonidas A.
Published: June 2023

Multiple endocrine neoplasia type 2 (MEN2) is an autosomal dominant (AD) condition with very high penetrance and expressivity. It is characterized into three clinical entities recognized as MEN2A, MEN2B, and familial medullary thyroid carcinoma (FMTC). In both MEN2A and MEN2B, there is a manifestation of multicentric tumor formation in the major organs such as the thyroid, parathyroid, and adrenal glands where the RET proto-oncogene is expressed. The FMTC form differs from MEN2A and MEN2B, since medullary thyroid carcinoma (MTC) is the only feature observed. In this present brief report, we demonstrate a collection of RET proto-oncogene genotype data from countries around the Mediterranean Basin with variable characteristics. As expected, a great extent of the Mediterranean RET proto-oncogene genotype data resemble the data reported globally. Most interestingly, higher frequencies are observed in the Mediterranean region for specific pathogenic RET variants as a result of local prevalence. The latter can be explained by founder effect phenomena. The Mediterranean epidemiological data that are presented herein are very important for domestic patients, their family members’ evaluation, and ultimately their treatment.
The Use of Aluminosilicate Ash Microspheres from Waste Ash and Slag Mixtures in Gypsum-Lime Compositions
Authors: Petropavlovskaya Victoria , Zavadko Maria , Novichenkova Tatiana , Petropavlovskii Kirill , Sulman Mikhail
Published: June 2023

The article considered the issues of the modification of gypsum stone to improve its performance properties. The influence of mineral additives on the physical and mechanical characteristics of the modified gypsum composition is described. The composition of the gypsum mixture included slaked lime and an aluminosilicate additive in the form of ash microspheres. It was isolated from ash and slag waste from fuel power plants as a result of their enrichment. This made it possible to reduce the carbon content in the additive to 3%. Modified compositions of the gypsum composition are proposed. The binder was replaced with an aluminosilicate microsphere. Hydrated lime was used to activate it. Its content varied: 0, 2, 4, 6, 8 and 10% of the weight of the gypsum binder. Replacing the binder with an aluminosilicate product for the enrichment of ash and slag mixtures made it possible to improve the structure of the stone and increase its operational properties. The compressive strength of the gypsum stone was 9 MPa. This is more than 100% higher than the strength of the control composition of gypsum stone. Studies have confirmed the effectiveness of using an aluminosilicate additive—a product of enrichment of ash and slag mixtures. The use of an aluminosilicate component for the production of modified gypsum mixtures allows the saving of gypsum resources. Developed formulations of gypsum compositions using aluminosilicate microspheres and chemical additives provide the specified performance properties. This makes it possible to use them in the production of self-leveling floors, plastering and puttying works. Replacing traditional compositions with a new composition based on waste has a positive effect on the preservation of the natural environment and contributes to the formation of comfortable conditions for human habitation.
Decentralized Adaptive Quantized Dynamic Surface Control for a Class of Flexible Hypersonic Flight Vehicles with Input Quantization
Authors: Zhao Wenyan , Lu Zeyu , Bi Zijian , Zhong Cheng , Tian Dianxiong , Zhang Yanhui , Zhang Xiuyu , Zhu Guoqiang
Published: June 2023

A control strategy for a certain class of hypersonic flight aircraft dynamic models with unknown parameters is proposed in this article. The strategy is adaptive dynamic surface input quantization control. To address the issues in conventional inversion control, a first-order low-pass filter and an adaptive parameter minimum learning law are introduced in the control system design process. This method has the following features: (1) it solves the problem of repeated differentiation of the virtual control law in the conventional back-stepping method, greatly simplifying the control law structure; (2) by using the norm of the neural network weight vector as the adaptive adjustment parameter instead of updating each element online, the number of adaptive adjustment parameters is significantly reduced, improving the execution efficiency of the controller; (3) the introduced hysteresis quantizer overcomes the disadvantage of the quantization accuracy deterioration when the input value is too low in the logarithm quantizer, improving the accuracy of the quantizer. Stability analysis has shown that all signals in the closed-loop system are semi-globally uniformly bounded, and simulation results have verified the effectiveness of the proposed adaptive quantized control scheme.
Fused Filament Fabrication and Injection Moulding of Plastic Packaging: An Environmental and Financial Comparative Assessment
Authors: Bezzina Cheryl Marie , Refalo Paul
Published: June 2023

The drive towards smart and sustainable manufacturing is leading companies to opt for environmentally conscious technologies. This study assesses the environmental and financial feasibility of using additive manufacturing, in this case, fused filament fabrication (FFF), instead of injection moulding (IM) to mass-produce cosmetic plastic packaging. Using a life cycle assessment (LCA), the environmental impacts of the raw material production and manufacturing processes were assessed for both technologies. The results showed that using FFF creates a five times greater environmental impact, with printing energy consumption generating 80% of the impact. Using costing models, the cost per product produced using IM and FFF was evaluated, and the models showed that the raw material costs comprise the highest share in both cases. A net present value (NPV) model over twelve years indicated that the FFF NPV was seventeen times higher than that of IM. When testing for quality, the packages produced using IM were superior overall. This study concludes that FFF is more expensive and environmentally impactful when compared to IM.
Iatrogenic Pneumothorax during Acupuncture: Case Report
Authors: Chiu Wen-Shan , Lu Yu-Wen , Lien Ting-Hsuan
Published: June 2023

Acupuncture treatment in local areas is commonly used to treat pain or soreness; however, acupuncture around the neck or shoulder may be a risk factor for pneumothorax. Herein, we report two cases of iatrogenic pneumothorax after acupuncture. These points indicate that physicians should be aware of these risk factors through history-taking before acupuncture. Chronic pulmonary diseases, such as chronic bronchitis, emphysema, tuberculosis, lung cancer, pneumonia, and thoracic surgery, may be associated with a higher risk of iatrogenic pneumothorax after acupuncture. Even if the incidence of pneumothorax should be low under caution and fully evaluated, it is still recommended to arrange further imaging examinations to rule out the possibility of iatrogenic pneumothorax.
A Review of the Characteristics of Clinical Trials and Potential Medications for Alcohol Dependence: Data Analysis from ClinicalTrials.gov
Authors: Alshehri Fahad S.
Published: June 2023

Objective. This study provides a comprehensive analysis of the characteristics of clinical trials related to alcohol dependence that are registered on ClinicalTrials.gov. Methods. All ClinicalTrials.gov trials registered up to 1 January 2023 were examined, focusing on trials that involved alcohol dependence. All 1295 trials were summarized by presenting their characteristics and results and reviewed most intervention drugs used in the treatment of alcohol dependence. Results. The study analysis identified a total of 1295 clinical trials registered on ClinicalTrials.gov that were focused on alcohol dependence. Of these, 766 trials had been completed, representing 59.15% of the total, while 230 trials were currently recruiting participants, accounting for 17.76% of the total. None of the trials had yet been approved for marketing. The majority of the studies included in this analysis were interventional studies (1145 trials, or 88.41%), which accounted for most of the patients enrolled in the trials. In contrast, observational studies represented only a small portion of the trials (150 studies, or 11.58%) and involved a smaller number of patients. In terms of geographic distribution, the majority of registered studies were located in North America (876 studies, or 67.64%), while only a small number of studies were registered in South America (7 studies, or 0.54%). Conclusions. The purpose of this review is to provide a basis for the treatment of alcohol dependence and prevention of its onset through an overview of clinical trials registered at ClinicalTrials.gov. It also offers essential information for future research to guide future studies.
Evaluation of Vertical Profiles and Atmospheric Boundary Layer Structure Using the Regional Climate Model CCLM during MOSAiC
Authors: Heinemann Günther , Schefczyk Lukas , Zentek Rolf , Brooks Ian M. , Dahlke Sandro , Walbröl Andreas
Published: June 2023

Regional climate models are a valuable tool for the study of the climate processes and climate change in polar regions, but the performance of the models has to be evaluated using experimental data. The regional climate model CCLM was used for simulations for the MOSAiC period with a horizontal resolution of 14 km (whole Arctic). CCLM was used in a forecast mode (nested in ERA5) and used a thermodynamic sea ice model. Sea ice concentration was taken from AMSR2 data (C15 run) and from a high-resolution data set (1 km) derived from MODIS data (C15MOD0 run). The model was evaluated using radiosonde data and data of different profiling systems with a focus on the winter period (November–April). The comparison with radiosonde data showed very good agreement for temperature, humidity, and wind. A cold bias was present in the ABL for November and December, which was smaller for the C15MOD0 run. In contrast, there was a warm bias for lower levels in March and April, which was smaller for the C15 run. The effects of different sea ice parameterizations were limited to heights below 300 m. High-resolution lidar and radar wind profiles as well as temperature and integrated water vapor (IWV) data from microwave radiometers were used for the comparison with CCLM for case studies, which included low-level jets. LIDAR wind profiles have many gaps, but represent a valuable data set for model evaluation. Comparisons with IWV and temperature data of microwave radiometers show very good agreement.
Antireflection Structures for VIS and NIR on Arbitrarily Shaped Fused Silica Substrates with Colloidal Polystyrene Nanosphere Lithography
Authors: Schmelz David , Jia Guobin , Käsebier Thomas , Plentz Jonathan , Zeitner Uwe Detlef
Published: June 2023

Antireflective (AR) nanostructures offer an effective, broadband alternative to conventional AR coatings that could be used even under extreme conditions. In this publication, a possible fabrication process based on colloidal polystyrene (PS) nanosphere lithography for the fabrication of such AR structures on arbitrarily shaped fused silica substrates is presented and evaluated. Special emphasis is placed on the involved manufacturing steps in order to be able to produce tailored and effective structures. An improved Langmuir-Blodgett self-assembly lithography technique enabled the deposition of 200 nm PS spheres on curved surfaces, independent of shape or material-specific characteristics such as hydrophobicity. The AR structures were fabricated on planar fused silica wafers and aspherical planoconvex lenses. Broadband AR structures with losses (reflection + transmissive scattering) of <1% per surface in the spectral range of 750–2000 nm were produced. At the best performance level, losses were less than 0.5%, which corresponds to an improvement factor of 6.7 compared to unstructured reference substrates.
Relevance of the Microbiota on Ocular Diseases: A Bibliometric and Citation Network Analysis
Authors: Sanchez-Tena Miguel Angel , Galvez Beatriz G. , Martinez-Perez Clara , Alvarez-Peregrina Cristina
Published: June 2023

Background: This study aimed to analyze the relevance of different publications about microbiota on ocular diseases and their authors through a citation network analysis. In addition, the different research areas and the most cited publications have been identified. Methods: The bibliographic search was carried out through the Web of Science (WOS) database, using the following search term: “microbiota AND (vision OR eye OR visual)” for the period between 1995 and December 2022. The Citation Network Explorer and the CiteSpace software have been used to analyze the different publications. Results: 705 publications were found in the field of microbiota on ocular diseases, together with 1014 citation networks. The year 2022 was the year with more publications. The first authors with the highest number of publications in the microbiota on the ocular surface field were Chisari G, Chisari CG, and Li Y. This field is multidisciplinary, highlighting “microbiology” and “ophthalmology” as the main research areas. Publications were clustered into three main groups allowing the identification of the main research topics in this field. The principal was the composition and diversity of the bacterial community on the ocular surface of patients with several pathologies. Conclusion: It could be useful for researchers to choose suitable collaborators or projects to promote their research on the role of microbiota on ocular diseases, as well as to know the main research topics that are of major interest today.