bluuebunny/crossref_metadata_2025 huggingface dataset:
Barriers to the Adoption of Modular Construction in Portugal: An Interpretive Structural Modeling Approach
Authors: Ribeiro Adriana Machado , Arantes Amílcar , Cruz Carlos Oliveira
Published: September 2022

Modular construction is the most advanced method of off-site construction available, generating fully-fitted modules with up to 80% of the work completed in a controlled environment prior to their transportation and installation on-site. The adoption of modular construction (AMC) has increased in several countries worldwide. However, in smaller construction markets, the adoption rates remain low, and the industry is still heavily reliant on traditional labor-intensive methods. The main research question for this study is: What are the main barriers (including the root barriers) to the adoption of modular construction in Portugal? The methodology used was a mixed methods research (MMR) approach, trying to understand if there are any specificities in the Portuguese (European Union) markets compared with the more well-documented North American and Asian markets. First, a literature review followed by a survey were used to prioritize a list of 15 critical barriers to the AMC. The results and interrelations between the barriers were analyzed through an interpretive structural modeling (ISM) approach and an impact matrix cross-reference multiplication applied to a classification (MICMAC) analysis. The combined analyses show that the main barriers are low levels of R&D, a lack of accredited organizations to certify the quality of the manufactured components and the industry’s unwillingness to innovate.
Employment among Childhood Cancer Survivors: A Systematic Review and Meta-Analysis
Authors: Godono Alessandro , Felicetti Francesco , Conti Alessio , Clari Marco , Dionisi-Vici Margherita , Gatti Filippo , Ciocan Catalina , Pinto Tommaso , Arvat Emanuela , Brignardello Enrico , Fagioli Franca , Pira Enrico
Published: September 2022

To date, there are heterogeneous studies related to childhood cancer survivors’ (CCS) employment rates. Given the importance of this topic, we aimed to perform a systematic review and meta-analysis to investigate the prevalence of employment among CCS and to examine its association with socio-demographic and clinical factors. We followed the PRISMA guidelines to search for pertinent articles in relevant electronic databases. Eighty-nine articles comprising 93 cohorts were included. The overall prevalence of employment was 66% (CI: 95% 0.63–0.69). Subgroup meta-analyses showed that lower rates were found for central nervous system tumor survivors (51%, CI: 95% 0.43–0.59), and for CCS treated with cranial-radiotherapy (53%, CI: 95% 0.42–0.64) or haematopoietic stem-cell transplantation (56%, CI: 95% 0.46–0.65). The studies conducted in Asia highlighted employment rates of 47% (CI: 95%, 0.34–0.60). Univariate meta-regressions identified the following socio-demographic factors associated with higher rates of employment: a female gender (p = 0.046), a higher mean age at the time of investigation (p = 0.00), a longer time since diagnosis (p = 0.00), a higher educational level (p = 0.03), and a married status (p = 0.00). In conclusion, this systematic review and meta-analysis provides evidence that two-thirds of CCS are employed worldwide. Identifying vulnerable groups of CCS may allow for the design of multidisciplinary support strategies and interventions to promote employment in this population.
The Clinical Impact of Methylated Homeobox A9 ctDNA in Patients with Non-Resectable Biliary Tract Cancer Treated with Erlotinib and Bevacizumab
Authors: Andersen Line Bechsgaard , Mahler Marit Sofie Kjær , Andersen Rikke Fredslund , Jensen Lars Henrik , Raunkilde Louise
Published: September 2022

Methylated homeobox A9 (meth-HOXA9) is tumor specific and has been suggested as a prognostic biomarker in several types of cancer. ctDNA measured as meth-HOXA9 may be a valuable biomarker in the decision-making process about last-line treatment of biliary tract cancer (BTC). The aim of the study was to investigate the clinical impact of meth-HOXA9 in plasma from patients receiving erlotinib and bevacizumab for late-stage BTC and to investigate the treatment effect and adverse events. Droplet digital PCR was applied to detect meth-HOXA9 in 39 patients. Response rates were registered according to RECIST (1.1) and adverse events according to Common Terminology Criteria for Adverse Events Version 4.0 (CTCAE (4.0)). Endpoints were progression-free survival (PFS), overall survival (OS), response rate, and toxicity. A significant difference in PFS and OS between patients with increasing and non-increasing meth-HOXA9 was detected after one treatment cycle, hazard ratio (HR) 12.4 (p < 0.0001) and HR 2.75 (p = 0.04), respectively. The most common adverse events of erlotinib were fatigue, pain, and rash, and those of bevacizumab were bleeding and wounds. This study found meth-HOXA9 to be negatively associated with survival in patients with late-stage BTC. Hence, meth-HOXA9 may guide early discontinuation of ineffective treatment.
Biological and Clinical Aspects of Metastatic Spinal Tumors
Authors: Litak Jakub , Czyżewski Wojciech , Szymoniuk Michał , Sakwa Leon , Pasierb Barbara , Litak Joanna , Hoffman Zofia , Kamieniak Piotr , Roliński Jacek
Published: September 2022

Spine metastases are a common life-threatening complication of advanced-stage malignancies and often result in poor prognosis. Symptomatic spine metastases develop in the course of about 10% of malignant neoplasms. Therefore, it is essential for contemporary medicine to understand metastatic processes in order to find appropriate, targeted therapeutic options. Thanks to continuous research, there appears more and more detailed knowledge about cancer and metastasis, but these transformations are extremely complicated, e.g., due to the complexity of reactions, the variety of places where they occur, or the participation of both tumor cells and host cells in these transitions. The right target points in tumor metastasis mechanisms are still being researched; that will help us in the proper diagnosis as well as in finding the right treatment. In this literature review, we described the current knowledge about the molecular pathways and biomarkers engaged in metastatic processes involving the spine. We also presented a current bone-targeted treatment for spine metastases and the emerging therapies targeting the discussed molecular mechanisms.
Dietary Inflammatory Index and Mortality from All Causes, Cardiovascular Disease, and Cancer: A Prospective Study
Authors: Liang Zhen , Feng Yanfei , Shivappa Nitin , Hebert James R. , Xu Xin
Published: September 2022

The Energy-adjusted Dietary Inflammatory Index (E-DIITM) is a comprehensive, literature-derived index for assessing the effect of dietary constituents on inflammatory biomarkers and inflammation-related chronic diseases. Several studies have examined the association between E-DII scores and mortality, with results that vary across populations. Therefore, in the present study, we aimed to investigate the potential association between E-DII scores and all-cause, cardiovascular disease (CVD), and cancer mortality using data from the Prostate, Lung, Colorectal and Ovarian (PLCO) Screening Trial. E-DII scores, calculated based on a food-frequency questionnaire, were analyzed both as a continuous variable and after categorization into quintiles. A multivariate Cox proportional hazards model was used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs). A total of 101,832 individuals were included, with 24,141 deaths recorded after a median of 17.0 years of follow-up. In multivariable-adjusted analyses, the E-DII score was significantly associated with all-cause mortality. The HR (95% CI) in the highest E-DII quintile compared to the lowest quintile was 1.23 (1.18–1.29). The E-DII was also statistically related to CVD mortality (Q5 vs. Q1; HR, 1.30 [95% CI, 1.20–1.41]) and cancer mortality (Q5 vs. Q1; HR, 1.14 [95% CI, 1.06–1.24]). Similar results were obtained from sensitivity analyses and subgroup analyses. In conclusion, the inflammatory potential of the diet, as calculated by the E-DII, was significantly associated with overall and CVD- and cancer-specific mortality risk in the PLCO study.
Pancreatic Cancer Cell-Derived Exosomes Promote Lymphangiogenesis by Downregulating ABHD11-AS1 Expression
Authors: Zhou Xulin , Zhong Fengyun , Yan Yongmin , Wu Sihui , Wang Huizhi , Liu Junqiang , Li Feifan , Cui Dawei , Xu Min
Published: September 2022

Research on pancreatic cancer microbiomes has attracted attention in recent years. The current view is that enriched microbial communities in pancreatic cancer tissues may affect pancreatic cancer metastasis, including lymph node (LN) metastasis. Similar to carriers of genetic information between cells, such as DNA, mRNA, protein, and non-coding RNA, exosomes are of great importance in early LN metastasis in tumors, including pancreatic cancer. Our previous study showed that the long non-coding RNA ABHD11-AS1 was highly expressed in tissues of patients with pancreatic cancer, and was correlated with patient survival time. However, the role of ABHD11-AS1 in pancreatic cancer LN metastasis has rarely been studied. Hence, in this paper we confirmed that exosomes derived from pancreatic cancer cells could promote lymphangiogenesis in vitro and in vivo, and that the mechanism was related to the downregulation of ABHD11-AS1 expression in lymphatic endothelial cells, and to the enhancement of their ability to proliferate, migrate, and form tubes. These findings preliminarily show a new mechanism by which pancreatic cancer cells regulate peripheral lymphangiogenesis, providing a new therapeutic strategy for inhibiting LN metastasis in pancreatic cancer.
Not a Mistake but a Feature: Promiscuous Activity of Enzymes Meeting Mycotoxins
Authors: Lyagin Ilya , Stepanov Nikolay , Maslova Olga , Senko Olga , Aslanli Aysel , Efremenko Elena
Published: September 2022

Mycotoxins are dangerous compounds and find multiple routes to enter living bodies of humans and animals. To solve the issue and degrade the toxicants, (bio)catalytic processes look very promising. Hexahistidine-tagged organophosphorus hydrolase (His6-OPH) is a well-studied catalyst for degradation of organophosphorus neurotoxins and lactone-containing quorum-sensing signal molecules. Moreover, the catalytic characteristics in hydrolysis of several mycotoxins (patulin, deoxynivalenol, zearalenone, and sterigmatocystin) were studied in this investigation. The best Michaelis constant and catalytic constant were estimated in the case of sterigmatocystin and patulin, respectively. A possible combination of His6-OPH with inorganic sorbents treated by low-temperature plasma was investigated. Further, enzyme–polyelectrolyte complexes of poly(glutamic acid) with His6-OPH and another enzymatic mycotoxin degrader (thermolysin) were successfully used to modify fiber materials. These catalytically active prototypes of protective materials appear to be useful for preventing surface contact and exposure to mycotoxins and other chemicals that are substrates for the enzymes used.
Catalytic Dehydration of Isopropanol to Propylene
Authors: Dubois Jean-Luc , Postole Georgeta , Silvester Lishil , Auroux Aline
Published: September 2022

Catalytic dehydration of isopropanol to propylene is a common reaction in laboratories to characterize the acid–base properties of catalysts. When acetone is produced, it is the sign of the presence of basic active sites, while propylene is produced on the acid sites. About 2/3rd of the world production of isopropanol is made from propylene, and the other third is made from acetone hydrogenation. Since the surplus acetone available on the market is mainly a coproduct of phenol synthesis, variations in the demand for phenol affect the supply position of acetone and vice versa. High propylene price and low demand for acetone should revive the industrial interest in acetone conversion. In addition, there is an increasing interest in the production of acetone and isopropanol from CO/CO2 via expected more environmentally friendly biochemical conversion routes. To preserve phenol process economics, surplus acetone should be recycled to propylene via the acetone hydrogenation and isopropanol dehydration routes. Some critical impurities present in petrochemical propylene are avoided in the recycling process. In this review, the selection criteria for the isopropanol dehydration catalysts at commercial scale are derived from the patent literature and analyzed with academic literature. The choice of the process conditions, such as pressure, temperature and gas velocity, and the catalysts’ properties such as pore size and acid–base behavior, are critical factors influencing the purity of propylene. Dehydration of isopropanol under pressure facilitates the downstream separation of products and the isolation of propylene to yield a high-purity “polymer grade”. However, it requires to operate at a higher temperature, which is a challenge for the catalyst’s lifetime; whereas operation at near atmospheric pressure, and eventually in a diluted stream, is relevant for applications that would tolerate a lower propylene purity (chemical grade).
Neuroinflammation in Dementia—Therapeutic Directions in a COVID-19 Pandemic Setting
Authors: Łuc Mateusz , Woźniak Marta , Rymaszewska Joanna
Published: September 2022

Although dementia is a heterogenous group of diseases, inflammation has been shown to play a central role in all of them and provides a common link in their pathology. This review aims to highlight the importance of immune response in the most common types of dementia. We describe molecular aspects of pro-inflammatory signaling and sources of inflammatory activation in the human organism, including a novel infectious agent, SARS-CoV-2. The role of glial cells in neuroinflammation, as well as potential therapeutic approaches, are then discussed. Peripheral immune response and increased cytokine production, including an early surge in TNF and IL-1β concentrations activate glia, leading to aggravation of neuroinflammation and dysfunction of neurons during COVID-19. Lifestyle factors, such as diet, have a large impact on future cognitive outcomes and should be included as a crucial intervention in dementia prevention. While the use of NSAIDs is not recommended due to inconclusive results on their efficacy and risk of side effects, the studies focused on the use of TNF antagonists as the more specific target in neuroinflammation are still very limited. It is still unknown, to what degree neuroinflammation resulting from COVID-19 may affect neurodegenerative process and cognitive functioning in the long term with ongoing reports of chronic post-COVID complications.
HIV-Associated Insults Modulate ADAM10 and Its Regulator Sirtuin1 in an NMDA Receptor-Dependent Manner
Authors: Lopez Lloreda Claudia , Chowdhury Sarah , Ghura Shivesh , Alvarez-Periel Elena , Jordan-Sciutto Kelly
Published: September 2022

Neurologic deficits associated with human immunodeficiency virus (HIV) infection impact about 50% of persons with HIV (PWH). These disorders, termed HIV-associated neurocognitive disorders (HAND), possess neuropathologic similarities to Alzheimer’s disease (AD), including intra- and extracellular amyloid-beta (Aβ) peptide aggregates. Aβ peptide is produced through cleavage of the amyloid precursor protein (APP) by the beta secretase BACE1. However, this is precluded by cleavage of APP by the non-amyloidogenic alpha secretase, ADAM10. Previous studies have found that BACE1 expression was increased in the CNS of PWH with HAND as well as animal models of HAND. Further, BACE1 contributed to neurotoxicity. Yet in in vitro models, the role of ADAM10 and its potential regulatory mechanisms had not been examined. To address this, primary rat cortical neurons were treated with supernatants from HIV-infected human macrophages (HIV/MDMs). We found that HIV/MDMs decreased levels of both ADAM10 and Sirtuin1 (SIRT1), a regulator of ADAM10 that is implicated in aging and in AD. Both decreases were blocked with NMDA receptor antagonists, and treatment with NMDA was sufficient to induce reduction in ADAM10 and SIRT1 protein levels. Furthermore, decreases in SIRT1 protein levels were observed at an earlier time point than the decreases in ADAM10 protein levels, and the reduction in SIRT1 was reversed by proteasome inhibitor MG132. This study indicates that HIV-associated insults, particularly excitotoxicity, contribute to changes of APP secretases by downregulating levels of ADAM10 and its regulator.
CRISPR-Based Therapeutic Gene Editing for Duchenne Muscular Dystrophy: Advances, Challenges and Perspectives
Authors: Chen Guofang , Wei Tingyi , Yang Hui , Li Guoling , Li Haisen
Published: September 2022

Duchenne muscular dystrophy (DMD) is a severe neuromuscular disease arising from loss-of-function mutations in the dystrophin gene and characterized by progressive muscle degeneration, respiratory insufficiency, cardiac failure, and premature death by the age of thirty. Albeit DMD is one of the most common types of fatal genetic diseases, there is no curative treatment for this devastating disorder. In recent years, gene editing via the clustered regularly interspaced short palindromic repeats (CRISPR) system has paved a new path toward correcting pathological mutations at the genetic source, thus enabling the permanent restoration of dystrophin expression and function throughout the musculature. To date, the therapeutic benefits of CRISPR genome-editing systems have been successfully demonstrated in human cells, rodents, canines, and piglets with diverse DMD mutations. Nevertheless, there remain some nonignorable challenges to be solved before the clinical application of CRISPR-based gene therapy. Herein, we provide an overview of therapeutic CRISPR genome-editing systems, summarize recent advancements in their applications in DMD contexts, and discuss several potential obstacles lying ahead of clinical translation.
Oral Health and Knowledge among Postpartum Women
Authors: Ben David Mordechai , Callen Yaffa , Eliasi Hila , Peretz Benjamin , Odeh-Natour Rasha , Ben David Hadani Michal , Blumer Sigalit
Published: September 2022

Oral health behavior and risks during pregnancy and after birth affect the oral health of babies and toddlers. We examined the oral and gingival health and caries prevalence of 150 postpartum women shortly after giving birth and assessed their knowledge of oral hygiene using a questionnaire. We also compared the oral health knowledge of nulliparous and multiparous women. Although most participants (98.0%) understood the importance of maintaining oral hygiene in children, their overall knowledge of oral health was medium–low, regardless of the number of previous pregnancies. Only 4.6% of women received oral health advice from their obstetrician during their pregnancy. Most participants had a high gingival index score, which correlated with dental pain during pregnancy. In contrast, the number of decayed, missing and filled teeth was significantly lower in first-time mothers. There was a statistically significant positive correlation between women who regularly visit their dentist and those who regularly take their children to the dentist. Expecting mothers should be educated about their own oral health and that of their developing fetus and children. Raising awareness among obstetricians with regards to this topic may be an effective way to achieve this.
Diagnosis, Treatment and Prognosis of Primary Pulmonary NUT Carcinoma: A Literature Review
Authors: Yuan Jiaqian , Xu Zhili , Guo Yong
Published: September 2022

NUT carcinoma is a rare, highly lethal cancer characterized with the rearrangement of the nuclear protein in testis (NUT) gene on chromosome 15q14, which primarily occurs in the midline organs. Primary pulmonary NUT carcinoma (NC) lacks characteristic clinical manifestations, which leads to the high rate of misdiagnose and nonstandard treatment. To date, fewer than one hundred cases have been reported worldwide. Here, a comprehensive literature search involving a total of 35 articles with 55 patients was conducted in this paper. We reviewed and analyzed the associated clinical and pathological characteristics, the efficacy of various treatment options and the prognosis. Pulmonary NC mainly occurred in middle young-aged men (median age, 36) with no smoking history (2:1) and would present with symptoms of cough (63.6%), dyspnea (29.5%), chest pain (18.2%) and hemoptysis (18.2%). The initial imaging frequently revealed large and irregular lesions in the lower lobe (46.5%) of the left or right lungs; lymph node metastasis was also prevalent (91.9%). A focal squamous differentiation with abrupt keratinization often occurred in the undifferentiated or poorly differentiated (93.75%) tumor cells, with abundant necrosis and numerous neutrophils infiltrated. The mean overall survival (OS) in patients of this malignant disease was 6.21 months, and the median OS was 4.4 months. According to our results, this disease is sensitive to radiotherapy, and chemoradiotherapy (either concurrent chemoradiotherapy or sequential chemoradiotherapy) was the most efficient therapeutic regimen to prolong the OS of patients with pulmonary NC.
Issues and Prospects of Current Endoscopic Treatment Strategy for Superficial Non-Ampullary Duodenal Epithelial Tumors
Authors: Suwa Tetsuya , Yoshida Masao , Ono Hiroyuki
Published: September 2022

An increasing number of duodenal tumors are being diagnosed over the years, leading to increased confusion regarding the choice of treatment options. Small-to-large tumors and histological types vary from adenoma to carcinoma, and treatment methods may need to be selected according to lesion characteristics. Because of its anatomic characteristics, complications are more likely to occur in the duodenum than in other gastrointestinal organs. Several reports have described the outcomes of conventional endoscopic mucosal resection, endoscopic submucosal dissection, cold snare polypectomy, underwater endoscopic mucosal resection, endoscopic full-thickness resection, and laparoscopic and endoscopic cooperative surgery for duodenal tumors. However, even in the guidelines set out by various countries, only the treatment methods are listed, and no clear treatment strategies are provided. Although there are few reports with a sufficiently high level of evidence, considering the currently available treatment options is essential. In this report, we reviewed previous reports on each treatment strategy, discussed the current issues and prospects, and proposed the best possible treatment strategy.
The Challenges of Patient Selection for Prostate Cancer Focal Therapy: A Retrospective Observational Multicentre Study
Authors: Paladini Alessio , Cochetti Giovanni , Colau Alexandre , Mouton Martin , Ciarletti Sara , Felici Graziano , Maiolino Giuseppe , Balzarini Federica , Sèbe Philippe , Mearini Ettore
Published: September 2022

Increased diagnoses of silent prostate cancer (PCa) have led to overtreatment and consequent functional side effects. Focal therapy (FT) applies energy to a prostatic index lesion treating only the clinically significant PCa focus. We analysed the potential predictive factors of FT failure. We collected data from patients who underwent robot-assisted radical prostatectomy (RARP) in two high-volume hospitals from January 2017 to January 2020. The inclusion criteria were: one MRI-detected lesion with a Gleason Score (GS) of ≤7, ≤cT2a, PSA of ≤10 ng/mL, and GS 6 on a random biopsy with ≤2 positive foci out of 12. Potential oncological safety of FT was defined as the respect of clinicopathological inclusion criteria on histology specimens, no extracapsular extension, and no biochemical, local, or metastatic recurrence within 12 months. To predict FT failure, we performed uni- and multivariate logistic regression. Sixty-seven patients were enrolled. The MRI index lesion median size was 11 mm; target lesions were ISUP grade 1 in 27 patients and ISUP grade 2 in 40. Potential FT failure occurred in 32 patients, and only the PSA value resulted as a predictive parameter (p < 0.05). The main issue for FT is patient selection, mainly because of multifocal csPCa foci. Nevertheless, FT could represent a therapeutic alternative for highly selected low-risk PCa patients.
Deep Learning Approaches for Long-Term Global Horizontal Irradiance Forecasting for Microgrids Planning
Authors: Medina-Santana Alfonso , Hewamalage Hansika , Cárdenas-Barrón Leopoldo
Published: September 2022

Providing sustainable energy to rural communities is considered in Sustainable Development Goal 7. Off-grid renewable energy systems arise as an affordable solution due to their portability and the availability of renewable sources for rural communities. In this work, to deal with the uncertainties of solar resources, we employ two deep learning models (feed forward and recurrent neural networks) to predict renewable sources in a long-term horizon. To this aim, the approach presented takes into account the necessity of a high enough resolution in the forecasting output. As a case study, we employ open source data for a location in Michoacan, Mexico as well as open source programming frameworks to ensure the replicability of the numerical experiments. The results show that our prediction model performs excellently with respect to the baseline methods (ARIMA, exponential smoothing, and seasonal naive) in terms of the evaluation metrics MASE (18.5% of reduction with respect to seasonal naive), RMSE (24.7%), WAPE (13.1%), MAE (12.9%), and APB (8.9%).
Estimating the Buckling Load of Steel Plates with Center Cut-Outs by ANN, GEP and EPR Techniques
Authors: Jayabalan Jagan , Dominic Manju , Ebid Ahmed M. , Soleymani Atefeh , Onyelowe Kennedy C. , Jahangir Hashem
Published: September 2022

Steel plates are used in the construction of various structures in civil engineering, aerospace, and shipbuilding. One of the main failure modes of plate members is buckling. Openings are provided in plates to accommodate various additional facilities and make the structure more serviceable. The present study examined the critical buckling load of rectangular steel plates with centrally placed circular openings and different support conditions. Various datasets were compiled from the literature and integrated into artificial intelligence techniques like Gene Expression Programming (GEP), Artificial Neural Network (ANN) and Evolutionary Polynomial Regression (EPR) to predict the critical buckling loads of the steel plates. The comparison of the developed models was conducted by determining various statistical parameters. The assessment revealed that the ANN model, with an R2 of 98.6% with an average error of 10.4%, outperformed the other two models showing its superiority in terms of better precision and less error. Thus, artificial intelligence techniques can be adopted as a successful technique for the prediction of the buckling load, and it is a sustainable method that can be used to solve practical problems encountered in the field of civil engineering, especially in steel structures.
Diagnostic Accuracy of Various Radiological Measurements in the Evaluation and Differentiation of Flatfoot: A Cross-Sectional Study
Authors: Khan Fayaz , Chevidikunnan Mohamed Faisal , Alsobhi Mashael Ghazi , Ahmed Israa Anees Ibrahim , Al-Lehidan Nada Saleh , Rehan Mohd , Alalawi Hashim Abdullah , Abduljabbar Ahmed H.
Published: September 2022

Arch angle is used to indicate flatfoot, but in some cases, it is not easily defined. The presence of flatfoot deformity remains difficult to diagnose due to a lack of reliable radiographic assessment tools. Although various assessment methods for flatfoot have been proposed, there is insufficient evidence to prove the diagnostic accuracy of the various tools. The main purpose of the study was to determine the best radiographic measures for flatfoot concerning the arch angle. Fifty-two feet radiographs from thirty-two healthy young females were obtained. Five angles and one index were measured using weight-bearing lateral radiographs; including arch angle, calcaneal pitch (CP), talar-first metatarsal angle (TFM), lateral talar angle (LTA), talar inclination angle (TIA) and navicular index (NI). Receiver-operating characteristics were generated to evaluate the flatfoot diagnostic accuracy for all radiographic indicators and Matthews correlation coefficient was calculated to determine the cutoff value for each measure. The strongest correlation was between arch angle and CP angle [r = −0.91, p ≤ 0.0001, 95% confidence interval (CI) (from −0.94 to −0.84)]. Also, significant correlations were found between arch angle and NI [r = 0.62, p ≤ 0.0001, 95% CI (0.42 to 0.76)], and TFM [r = 0.50, p ≤ 0.0001, 95% CI (from 0.266 to 0.68)]. Furthermore, CP (cutoff, 12.40) had the highest accuracy level with value of 100% sensitivity and specificity followed by NI, having 82% sensitivity and 89% specificity for the cutoff value of 9.90. In conclusion, CP angle is inversely correlated with arch angle and considered a significant indicator of flatfoot. Also, the NI is easy to define radiographically and could be used to differentiate flat from normal arched foot among young adults.
Pathological Nodal Staging Score for Gastric Signet Ring Cell Carcinoma: A Clinical Tool of Adequate Nodal Staging
Authors: Yu Chaoran , Zhou Zhiyuan , Liu Bin , Yao Danhua , Huang Yuhua , Wang Pengfei , Li Yousheng
Published: September 2022

Background: Gastric signet ring cell carcinoma (GSRCC) is a subset of gastric cancer with distinct histological and inconsistent prognosis outcome. Currently, the association between the adequate regional lymph node and proper nodal staging in GSRCC is rarely noticed. Materials and methods: Clinical data of GSRCC were retrieved from the Surveillance, Epidemiology, and End Results database. Beta-binomial distribution model was employed for the estimation of the probability of missing nodal disease, followed by the development of a nodal staging score (NSS). Results: A total of 561 GSRCC patients were included in this study, with 193 in lymph node-negative and 368 in lymph node-positive diagnoses. As the number of examined lymph nodes increased, the probability of missing nodal disease decreased rapidly, with T stage-specific curves. The probability of missing nodal disease in T4 was lower than that in T1. NSS calculation indicated that T1 stage patients commonly had NSS > 0.8. However, with the NSS of T2–T4 to reach 0.8, the number of examined lymph node was required to be larger than 12 in T2, 17 in T3 and 27 in T4. NSS ≥ 0.75 (quantile 75%) subgroup in T2–T4 subgroups tended to have better outcome; however, without significant prognostic value. Conclusions: NSS is served as a reliable and feasible tool in adequate nodal staging of GSRCC with statistical basis and provides further evidence for clinical decision making.
A Modular Grad-Div Stabilization Method for Time-Dependent Thermally Coupled MHD Equations
Authors: Li Xianzhu , Su Haiyan
Published: September 2022

In this paper, we consider a fully discrete modular grad-div stabilization algorithm for time-dependent thermally coupled magnetohydrodynamic (MHD) equations. The main idea of the proposed algorithm is to add an extra minimally intrusive module to penalize the divergence errors of velocity and improve the computational efficiency for increasing values of the Reynolds number and grad-div stabilization parameters. In addition, we provide the unconditional stability and optimal convergence analysis of this algorithm. Finally, several numerical experiments are performed and further indicated these advantages over the algorithm without grad-div stabilization.
Security Analysis of Sending or Not-Sending Twin-Field Quantum Key Distribution with Weak Randomness
Authors: Jiang Xiao-Lei , Wang Yang , Lu Yi-Fei , Li Jia-Ji , Zhou Chun , Bao Wan-Su
Published: September 2022

Sending-or-not sending twin-field quantum key distribution (SNS TF-QKD) has the advantage of tolerating large amounts of misalignment errors, and its key rate can exceed the linear bound of repeaterless quantum key distribution. However, the weak randomness in a practical QKD system may lower the secret key rate and limit its achievable communication distance, thus compromising its performance. In this paper, we analyze the effects of the weak randomness on the SNS TF-QKD. The numerical simulation shows that SNS TF-QKD can still have an excellent performance under the weak random condition: the secret key rate can exceed the PLOB boundary and achieve long transmission distances. Furthermore, our simulation results also show that SNS TF-QKD is more robust to the weak randomness loopholes than the BB84 protocol and the measurement-device-independent QKD (MDI-QKD). Our results emphasize that keeping the randomness of the states is significant to the protection of state preparation devices.
A Capacitorless Flipped Voltage Follower LDO with Fast Transient Using Dynamic Bias
Authors: Lu Yange , Chen Ming , Wang Kunyu , Yang Yanjun , Wang Haiyong
Published: September 2022

The output capacitorless low-dropout regulator (OCL-LDO) has developed rapidly in recent years. This paper presents a flipped voltage follower (FVF) OCL-LDO with fast transient response. By adding a dynamic bias circuit to the FVF circuit, the proposed LDO has the ability to quickly adjust the gate voltage of the power transistor, without extra power consumption. The proposed LDO was designed in 0.18 μm CMOS process. The simulation results show that the recovery time is 52 ns when the load changes from 0.1 mA to 20 mA with a slew rate of 20 mA/ps, while the quiescent current is 92 μA with 1 V regulated output. The undershoot and overshoot voltage are 242 mV and 250 mV, respectively.
An Improved Down-Scale Evaluation System for Capacitors Utilized in High-Power Three-Phase Inverters under Balanced and Unbalanced Load Conditions
Authors: Liao Yi-Hung , Lin Yen-Lun
Published: September 2022

The DC-link capacitors in an electronic power system are the main constraint of the power density and lifespan of the power converters. Evaluating the load life of capacitors working in severely adverse circumstances plays an important role in the design stages of the next-generation power converters. In this article, an improved evaluation system for the capacitors utilized in high-power three-phase voltage source inverters is proposed. The purpose of this article is to reproduce the same encountered stresses when a DC-link capacitor is used in a high-power inverter with pulse-width modulation. Hence, an improved down-scale evaluation system for the DC-link capacitors used in high-power three-phase inverter systems under balanced and unbalanced load conditions is proposed. Moreover, AC and DC analyses in the proposed evaluation system are conducted. The equivalent circuit and transfer functions are derived to verify the proposed evaluation system. Finally, a prototype system is constructed to facilitate the theoretical results as the verification.
Liquid Air Energy Storage Model for Scheduling Purposes in Island Power Systems
Authors: Rajabdorri Mohammad , Sigrist Lukas , Lobato Enrique
Published: September 2022

Moving towards clean energy generation seems essential. To do so, renewable energy penetration is growing in the power systems. Although energy sources such as wind and solar are clean, they are not available consistently. Using energy storage will help to tackle variability. Liquid air energy storage is gaining attention among different energy storage technologies, as it is a promising option for grid-scale energy storage. This paper presents a detailed mixed integer linear model of liquid air energy storage to be used in scheduling and planning problems. A comprehensive cycle diagram of different processes of liquid air energy storage is presented, and a model has been developed accordingly. Simulations of the proposed model are carried out for the power system of Tenerife island and compared with the basic models. Basic models overlook specific characteristics of liquid air energy storage systems, such as charging and discharging start energy. Results confirm that the use of simple models will lead to misleading conclusions and overestimate the economic benefits of liquid air energy storage.
Improvement of Ex Vitro Growing Completion of Highbush Blueberry (Vaccinium corymbosum L.) in Containers
Authors: Akimova Svetlana , Radzhabov Agamagomed , Esaulko Aleksandr , Samoshenkov Egor , Nechiporenko Ivan , Kazakov Pavel , Voskoboinikov Yurii , Matsneva Anna , Zubkov Aleksandr , Aisanov Timur
Published: September 2022

Highbush blueberry (Vaccinium corymbosum L.), originally a forest plant, is currently in need of improvement of clonal micropropagation technologies. It is known that the large percentage of propagated plants can be lost or damaged, not only at the stage of acclimatization to non-sterile conditions, but also during the growing completion stage. In fact, successful ex vitro regeneration of such plants is determined by their ability to produce new shoots that can adapt to new cultivation conditions. The lighting and ratio of nutrients under ex vitro conditions play an important role in the development of the plants’ photosynthetic capacity. The research revealed that LED grow lighting has a positive effect on the development of ex vitro plants of highbush blueberry (Vaccinium corymbosum L.) cv. Brigitta Blue, only at the initial stages of growing in 0.5-L containers. The results obtained have improved our understanding of lighting and mineral fertilizer’s impact on the development of ex vitro plants of the highbush blueberry (Vaccinium corymbosum L.) cv. Brigitta Blue in greenhouse conditions. This can be useful for providing blueberry planting stock and commercial use for large scale production.
Characterization of Microsatellite Distribution in Siamese Fighting Fish Genome to Promote Conservation and Genetic Diversity
Authors: Wattanadilokchatkun Pish , Panthum Thitipong , Jaisamut Kitipong , Ahmad Syed Farhan , Dokkaew Sahabhop , Muangmai Narongrit , Duengkae Prateep , Singchat Worapong , Srikulnath Kornsorn
Published: September 2022

The Siamese fighting fish (Betta splendens) is a well-known ornamental fish and emerging model species for studying animal morphology, physiology, and behavior. A key concern of betta inbreeding is the decline in genetic diversity resulting from commercial breeding programs. Therefore, it is essential to develop markers for understanding the genetic bases of the domestication and phenotypic diversification of this species. We utilized the previously assembled genome of Siamese fighting fish to identify and characterize microsatellites and compare their genomic organization across different species. We annotated 812,134 microsatellite loci spanning 30.70 Mb, accounting for 6.57% of the Siamese fighting fish genome. We performed in silico polymorphism screening of microsatellites in the Siamese fighting fish and related species and present these sequences as candidate markers for cross-species amplification. In addition, we successfully validated two microsatellite loci using PCR-based assays in different species, which can promote further genetic characterization of diverse betta lineages. The set of polymorphic markers identified in this study may facilitate the assessment of genetic diversity and population structure and marker-assisted selection, among other applications.
Antimicrobial Resistance in the Food Chain: Trends, Mechanisms, Pathways, and Possible Regulation Strategies
Authors: Samtiya Mrinal , Matthews Karl R. , Dhewa Tejpal , Puniya Anil Kumar
Published: September 2022

Antimicrobial resistance (AMR) remains of major interest for different types of food stakeholders since it can negatively impact human health on a global scale. Antimicrobial-resistant bacteria and/or antimicrobial resistance genes (transfer in pathogenic bacteria) may contaminate food at any stage, from the field to retail. Research demonstrates that antimicrobial-resistant bacterial infection(s) occur more frequently in low- and middle-income countries (LMICs) than in developed countries. Worldwide, foodborne pathogens are a primary cause of morbidity and mortality. The spread of pathogenic bacteria from food to consumers may occur by direct or indirect routes. Therefore, an array of approaches both at the national and international level to control the spread of foodborne pathogens and promote food safety and security are essential. Zoonotic microbes can spread through the environment, animals, humans, and the food chain. Antimicrobial drugs are used globally to treat infections in humans and animals and prophylactically in production agriculture. Research highlights that foods may become contaminated with AMR bacteria (AMRB) during the continuum from the farm to processing to retail to the consumer. To mitigate the risk of AMRB in humans, it is crucial to control antibiotic use throughout food production, both for animal and crop agriculture. The main inferences of this review are (1) routes by which AMRB enters the food chain during crop and animal production and other modes, (2) prevention and control steps for AMRB, and (3) impact on human health if AMR is not addressed globally. A thorough perspective is presented on the gaps in current systems for surveillance of antimicrobial use in food production and/ or AMR in the food chain.
Detailed Error Analysis for a Fractional Adams Method on Caputo–Hadamard Fractional Differential Equations
Authors: Green Charles Wing Ho , Yan Yubin
Published: September 2022

We consider a predictor–corrector numerical method for solving Caputo–Hadamard fractional differential equation over the uniform mesh logtj=loga+logtNajN,j=0,1,2,…,N with a≥1, where loga=logt0<logt1<…<logtN=logT is a partition of [loga,logT]. The error estimates under the different smoothness properties of the solution y and the nonlinear function f are studied. Numerical examples are given to verify that the numerical results are consistent with the theoretical results.
Collective Sensitivity and Collective Accessibility of Non-Autonomous Discrete Dynamical Systems
Authors: Pi Jingmin , Lu Tianxiu , Chen Yuanlin
Published: September 2022

The concepts of collectively accessible, collectively sensitive, collectively infinitely sensitive, and collectively Li–Yorke sensitive are defined in non-autonomous discrete systems. It is proved that, if the mapping sequence h1,∞=(h1,h2,…) is W-chaotic, then hn,∞=(hn,hn+1,…)(∀n∈N={1,2,…}) would also be W-chaotic. W-chaos represents one of the following five properties: collectively accessible, sensitive, collectively sensitive, collectively infinitely sensitive, and collectively Li–Yorke sensitive. Then, the relationship of chaotic properties between the product system (H1×H2,f1,∞×g1,∞) and factor systems (H1,f1,∞) and (H2,g1,∞) was presented. Furthermore, in this paper, it is also proved that, if the autonomous discrete system (X,h^) induced by the p-periodic discrete system (H,h1,∞) is W-chaotic, then the p-periodic discrete system (H,f1,∞) would also be W-chaotic.
Intrinsic Disorder in BAP1 and Its Association with Uveal Melanoma
Authors: Djulbegovic Mak B. , Taylor David J. , Uversky Vladimir N. , Galor Anat , Shields Carol L. , Karp Carol L.
Published: September 2022

Background: Specific subvariants of uveal melanoma (UM) are associated with increased rates of metastasis compared to other subvariants. BRCA1 (BReast CAncer gene 1)-associated protein-1 (BAP1) is encoded by a gene that has been linked to aggressive behavior in UM. Methods: We evaluated BAP1 for the presence of intrinsically disordered protein regions (IDPRs) and its protein–protein interactions (PPI). We evaluated specific sequence-based features of the BAP1 protein using a set of bioinformatic databases, predictors, and algorithms. Results: We show that BAP1’s structure contains extensive IDPRs as it is highly enriched in proline residues (the most disordered amino acid; p-value < 0.05), the average percent of predicted disordered residues (PPDR) was 57.34%, and contains 9 disorder-based binding sites (ie. molecular recognition features (MoRFs)). BAP1’s intrinsic disorder allows it to engage in a complex PPI network with at least 49 partners (p-value < 1.0 × 10−16). Conclusion: These findings show that BAP1 contains IDPRs and an intricate PPI network. Mutations in UM that are associated with the BAP1 gene may alter the function of the IDPRs embedded into its structure. These findings develop the understanding of UM and may provide a target for potential novel therapies to treat this aggressive neoplasm.
Free Fatty Acid Overload Targets Mitochondria: Gene Expression Analysis of Palmitic Acid-Treated Endothelial Cells
Authors: Raja Afraz Ahmad , Dandare Abdullahi , Khan Muhammad Jawad , Khan Muhammad Jadoon
Published: September 2022

Lipotoxicity is known to cause cellular dysfunction and death in non-adipose tissue. A major cause of lipotoxicity is the accumulation of saturated free fatty acids (FFA). Palmitic acid (PA) is the most common saturated fatty acid found in the human body. Endothelial cells form the blood vessels and are the first non-adipose cells to encounter FFA in the bloodstream. FFA overload has a direct impact on metabolism, which is evident through the changes occurring in mitochondria. To study these changes, the PA-treated human coronary artery endothelial cell (HCAEC) dataset was obtained from the Gene Expression Omnibus (GEO), and it was analyzed to obtain differentially expressed genes (DEGs) from the nucleus and mitochondria. Functional and pathway enrichment analyses were performed on DEGs. Results showed that nuclear and mitochondrial DEGs were implicated in several processes, e.g., reactive oxygen species (ROS) production, mitochondrial fusion and fission, Ca2+ sequestering, membrane transport, the electron transport chain and the process of apoptosis. To better understand the role of FFA in endothelial cell damage, these DEGs can lead to future experiments based on these findings.
Chloroplast Genome Analysis for Genetic Information and Authentication in Five Barleria Species
Authors: Kaewdaungdee Sanit , Sudmoon Runglawan , Tanee Tawatchai , Lee Shiou Yih , Chaveerach Arunrat
Published: September 2022

In order to authenticate the genomic information of Barleriacristata L., B. lupulina Lindl., B. repens Nees, B. siamensis Craib, and B. strigosa Willd, cp genomes were investigated. They revealed a general structure with a total size of 151,997–152,324 bp. The genomes encoded a total of 131 genes, including 86 CDS, 37 tRNA, and 8 rRNA genes. Other details found were as follows: different numbers and types of SSRs; identical gene content, which is adjacent to the border regions, except for B. strigosa, that revealed a shorter ndhF gene sequence and lacked the ycf1 gene; slightly different genetic distance values, which can be used for species identification; three distinct gaps of nucleotide variations between the species located at the intergenic spacer regions of the LSC and CDS of the SSC; three effective molecular markers derived from divergent hotspot regions, including the ccsA-ndhD, ndhA-ndhH-rps15, and ycf1. The genetic relationships derived from the cp genome and the CDS phylogenetic trees of Barleria and the 13 genera in Acanthaceae and different families, Scrophulariaceae and Phrymaceae, showed similar results. The six Barleria species as monophyletic groups with inner and outer outgroups were found to have perfect discrimination. These results have helped to authenticate the five Barleria species and the six genera in Acanthaceae.