bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Environmental Sub-Inhibitory Concentrations of Antibiotics, Heavy Metals, and Biocides on the Emergence of Tolerance and Effects on the Mutant Selection Window in E. coli
Authors: Chukwu Kelechi B. , Abafe Ovokeroye A. , Amoako Daniel G. , Ismail Arshad , Essack Sabiha Y. , Abia Akebe L. K.
Published: September 2023

Bacteria’s ability to withstand the detrimental effects of antimicrobials could occur as resistance or tolerance with the minimum inhibitory concentration, the mutant prevention concentration, and the mutant selection window as salient concepts. Thus, this study assessed the impact of exposure to extremely high doses of ampicillin on the level of persistence and tolerance development in isolates previously exposed to different concentrations of selected antibiotics, biocides, and heavy metals. These isolates were previously exposed to oxytetracycline (OXYTET), amoxicillin (AMX), copper (Cu), zinc (Zn), benzalkonium chloride (BAC) 10, dimethylammonium chloride (DADMAC) 12 and a combination of all the individual pollutants (ALL). The isolates were exposed to very high concentrations (25 × MIC) of ampicillin, and their tolerance was calculated as the time required to kill 99.9% of the bacterial population (MDK99.9). The MDK99.9 increased by 30 to 50% in test isolates (DADMAC, OXYTET, Zinc = 28 h; BAC, Copper = 30 h; amoxycillin, ALL = 26 h) compared to the untreated control. BAC-exposed isolates decreased from 2.5 × 108 CFU/mL to 2.5 × 104 CFU/mL on the second day, displaying the highest tolerance increase. The tolerance appeared to originate from two sources, i.e., stochastic persistence and genetic-induced persistence, involving multiple genes with diverse mechanisms. The mutant selection window of the isolates to ampicillin, amoxicillin, and oxytetracycline also slightly increased compared to the control, indicating the selective survival of persister cells during the 30-day exposure. These findings indicate that bacterial exposure to sub-inhibitory concentrations of environmental chemical stressors may not always result in the development of antimicrobial resistance but could initiate this process by selecting persisters that could evolve into resistant isolates.
Enriched Aptamer Libraries in Fluorescence-Based Assays for Rikenella microfusus-Specific Gut Microbiome Analyses
Authors: Zhang Yiting , Xing Hu , Bolotnikov Grigory , Krämer Markus , Gotzmann Nina , Knippschild Uwe , Kissmann Ann-Kathrin , Rosenau Frank
Published: September 2023

Rikenella microfusus is an essential intestinal probiotic with great potential. The latest research shows that imbalance in the intestinal flora are related to the occurrence of various diseases, such as intestinal diseases, immune diseases, and metabolic diseases. Rikenella may be a target or biomarker for some diseases, providing a new possibility for preventing and treating these diseases by monitoring and optimizing the abundance of Rikenella in the intestine. However, the current monitoring methods have disadvantages, such as long detection times, complicated operations, and high costs, which seriously limit the possibility of clinical application of microbiome-based treatment options. Therefore, the intention of this study was to evolve an enriched aptamer library to be used for specific labeling of R. microfusus, allowing rapid and low-cost detection methods and, ultimately the construction of aptamer-based biosensors. In this study, we used Rikenella as the target bacterium for an in vitro whole Cell-SELEX (Systematic Evolution of Ligands by EXponential Enrichment) to evolve and enrich specific DNA oligonucleotide aptamers. Five other prominent anaerobic gut bacteria were included in this process for counterselection and served as control cells. The aptamer library R.m-R13 was evolved with high specificity and strong affinity (Kd = 9.597 nM after 13 rounds of selection). With this enriched aptamer library, R. microfusus could efficiently be discriminated from the control bacteria in complex mixtures using different analysis techniques, including fluorescence microscopy or fluorometric suspension assays, and even in human stool samples. These preliminary results open new avenues toward the development of aptamer-based microbiome bio-sensing applications for fast and reliable monitoring of R. microfusus.
Genomic Characterization of IMP-Producing Pseudomonas aeruginosa in Bulgaria Reveals the Emergence of IMP-100, a Novel Plasmid-Mediated Variant Coexisting with a Chromosomal VIM-4
Authors: Stoikov Ivan , Ivanov Ivan N. , Donchev Deyan , Teneva Deana , Dobreva Elina , Hristova Rumyana , Sabtcheva Stefana
Published: September 2023

Multidrug-resistant (MDR) Pseudomonas aeruginosa infections represent a major public health concern and require comprehensive understanding of their genetic makeup. This study investigated the first occurrence of imipenemase (IMP)-carrying P. aeruginosa strains from Bulgaria. Whole genome sequencing identified a novel plasmid-mediated IMP-100 allele located in a a novel In4886 integron embedded in a putative Tn7700 transposon. Two other closely related chromosomal IMP variants, IMP-13 and IMP-84, were also detected. The IMP-producers were resistant to last-line drugs including cefiderocol (CFDC) (two out of three) and susceptible to colistin. The IMP-13/84 cassettes were situated in a In320 integron inserted in a Tn5051-like transposon as previously reported. Lastly, the p4782-IMP plasmid rendered the PA01 transformant resistant to CFDC, suggesting a transferable CFDC resistance. A variety of virulence factors associated with adhesion, antiphagocytosis, iron uptake, and quorum sensing, as well as secretion systems, toxins, and proteases, were confirmed, suggesting significant pathogenic potential consistent with the observed strong biofilm formation. The emergence of IMP-producing MDR P. aeruginosa is alarming as it remains unsusceptible even to last-generation drugs like CFDC. Newly detected IMP-100 was even located in a CFDC-resistant XDR strain.
Perturbation of Quorum Sensing after the Acquisition of Bacteriophage Resistance Could Contribute to Novel Traits in Vibrio alginolyticus
Authors: Skliros Dimitrios , Droubogiannis Stavros , Kalloniati Chrysanthi , Katharios Pantelis , Flemetakis Emmanouil
Published: September 2023

Bacteria employ a wide range of molecular mechanisms to confer resistance to bacteriophages, and these mechanisms are continuously being discovered and characterized. However, there are instances where certain bacterial species, despite lacking these known mechanisms, can still develop bacteriophage resistance through intricate metabolic adaptation strategies, potentially involving mutations in transcriptional regulators or phage receptors. Vibrio species have been particularly useful for studying the orchestrated metabolic responses of Gram-negative marine bacteria in various challenges. In a previous study, we demonstrated that Vibrio alginolyticus downregulates the expression of specific receptors and transporters in its membrane, which may enable the bacterium to evade infection by lytic bacteriophages. In our current study, our objective was to explore how the development of bacteriophage resistance in Vibrio species disrupts the quorum-sensing cascade, subsequently affecting bacterial physiology and metabolic capacity. Using a real-time quantitative PCR (rt-QPCR) platform, we examined the expression pattern of quorum-sensing genes, auto-inducer biosynthesis genes, and cell density regulatory proteins in phage-resistant strains. Our results revealed that bacteriophage-resistant bacteria downregulate the expression of quorum-sensing regulatory proteins, such as LuxM, LuxN, and LuxP. This downregulation attenuates the normal perception of quorum-sensing peptides and subsequently diminishes the expression of cell density regulatory proteins, including LuxU, aphA, and LuxR. These findings align with the diverse phenotypic traits observed in the phage-resistant strains, such as altered biofilm formation, reduced planktonic growth, and reduced virulence. Moreover, the transcriptional depletion of aphA, the master regulator associated with low cell density, was linked to the downregulation of genes related to virulence. This phenomenon appears to be phage-specific, suggesting a finely tuned metabolic adaptation driven by phage–host interaction. These findings contribute to our understanding of the role of Vibrio species in microbial marine ecology and highlight the complex interplay between phage resistance, quorum sensing, and bacterial physiology.
Prediction of Reflection Seismic Low-Frequency Components of Acoustic Impedance Using Deep Learning
Authors: Jiang Lian , Castagna John P. , Zhang Zhao , Russell Brian
Published: September 2023

The unreliable prediction of the low-frequency components from inverted acoustic impedance causes uncertainty in quantitative seismic interpretation. To address this issue, we first calculate various seismic and geological attributes that contain low-frequency information, such as relative geological age, interval velocity, and integrated instantaneous amplitude. Then, we develop a method to predict the low-frequency content of seismic data using these attributes, their high-frequency components, and recurrent neural networks. Next, we test how to predict the low-frequency components using stacking velocity obtained from velocity analysis. Using all the attributes and seismic data, we propose a supervised deep learning method to predict the low-frequency components of the inverted acoustic impedance. The results obtained in both synthetic and real data cases show that the proposed method can improve the prediction accuracy of the low-frequency components of the inverted acoustic impedance, with the best improvement in a real data example of 57.7% compared with the impedance predicted using well-log interpolation.
A Review on Wine Flavour Profiles Altered by Bottle Aging
Authors: Zhang Di , Wei Ziyu , Han Yufeng , Duan Yaru , Shi Baohui , Ma Wen
Published: September 2023

The wine flavour profile directly determines the overall quality of wine and changes significantly during bottle aging. Understanding the mechanism of flavour evolution during wine bottle aging is important for controlling wine quality through cellar management. This literature review summarises the changes in volatile compounds and non-volatile compounds that occur during wine bottle aging, discusses chemical reaction mechanisms, and outlines the factors that may affect this evolution. This review aims to provide a deeper understanding of bottle aging management and to identify the current literature gaps for future research.
Anticancer Effects of Fucoxanthin through Cell Cycle Arrest, Apoptosis Induction, and Angiogenesis Inhibition in Triple-Negative Breast Cancer Cells
Authors: Ahmed Shade’ A. , Mendonca Patricia , Messeha Samia S. , Soliman Karam F. A.
Published: September 2023

The absence of progesterone receptors, estrogen receptors, and human epidermal growth factor receptor-2 restricts the therapy choices for treating triple-negative breast cancer (TNBC). Moreover, conventional medication is not highly effective in treating TNBC, and developing effective therapeutic agents from natural bioactive compounds is a viable option. In this study, the anticancer effects of the natural compound fucoxanthin were investigated in two genetically different models of TNBC cells: MDA-MB-231 and MDA-MB-468 cells. Fucoxanthin had a significant anticancer effect in both cell lines at a concentration range of 1.56–300 µM. The compound decreased cell viability in both cell lines with higher potency in MDA-MB-468 cells. Meanwhile, proliferation assays showed similar antiproliferative effects in both cell lines after 48 h and 72 h treatment periods. Flow cytometry and Annexin V-FITC apoptosis assay revealed the ability of fucoxanthin to induce apoptosis in MDA-MB-231 only. Cell cycle arrest analysis showed that the compound also induced cell cycle arrest at the G1 phase in both cell lines, accompanied by more cell cycle arrest in MDA-MB-231 cells at S-phase and a higher cell cycle arrest in the MDA-MB-468 cells at G2-phase. Wound healing and migration assay showed that in both cell lines, fucoxanthin prevented migration, but was more effective in MDA-MB-231 cells in a shorter time. In both angiogenic cytokine array and RT-PCR studies, fucoxanthin (6.25 µM) downregulated VEGF-A and -C expression in TNF-α-stimulated (50 ng/mL) MDA-MB-231, but not in MDA-MB-468 cells on the transcription and protein levels. In conclusion, this study shows that fucoxanthin was more effective in MDA-MB-231 TNBC cells, where it can target VEGF-A and VEGF-C, inhibit cell proliferation and cell migration, and induce cell cycle arrest and apoptosis—the most crucial cellular processes involved in breast cancer development and progression.
CCSD(T) Rotational Constants for Highly Challenging C5H2 Isomers—A Comparison between Theory and Experiment
Authors: Thimmakondu Venkatesan S. , Karton Amir
Published: September 2023

We evaluate the accuracy of CCSD(T) and density functional theory (DFT) methods for the calculation of equilibrium rotational constants (Ae, Be, and Ce) for four experimentally detected low-lying C5H2 isomers (ethynylcyclopropenylidene (2), pentatetraenylidene (3), ethynylpropadienylidene (5), and 2-cyclopropen-1-ylidenethenylidene (8)). The calculated rotational constants are compared to semi-experimental rotational constants obtained by converting the vibrationally averaged experimental rotational constants (A0, B0, and C0) to equilibrium values by subtracting the vibrational contributions (calculated at the B3LYP/jun-cc-pVTZ level of the theory). The considered isomers are closed-shell carbenes, with cumulene, acetylene, or strained cyclopropene moieties, and are therefore highly challenging from an electronic structure point of view. We consider both frozen-core and all-electron CCSD(T) calculations, as well as a range of DFT methods. We find that calculating the equilibrium rotational constants of these C5H2 isomers is a difficult task, even at the CCSD(T) level. For example, at the all-electron CCSD(T)/cc-pwCVTZ level of the theory, we obtain percentage errors ≤0.4% (Ce of isomer 3, Be and Ce of isomer 5, and Be of isomer 8) and 0.9–1.5% (Be and Ce of isomer 2, Ae of isomer 5, and Ce of isomer 8), whereas for the Ae rotational constant of isomers 2 and 8 and Be rotational constant of isomer 3, high percentage errors above 3% are obtained. These results highlight the challenges associated with calculating accurate rotational constants for isomers with highly challenging electronic structures, which is further complicated by the need to convert vibrationally averaged experimental rotational constants to equilibrium values. We use our best CCSD(T) rotational constants (namely, ae-CCSD(T)/cc-pwCVTZ for isomers 2 and 5, and ae-CCSD(T)/cc-pCVQZ for isomers 3 and 8) to evaluate the performance of DFT methods across the rungs of Jacob’s Ladder. We find that the considered pure functionals (BLYP-D3BJ, PBE-D3BJ, and TPSS-D3BJ) perform significantly better than the global and range-separated hybrid functionals. The double-hybrid DSD-PBEP86-D3BJ method shows the best overall performance, with percentage errors below 0.5% in nearly all cases.
In Situ N, O Co-Doped Nanoporous Carbon Derived from Mixed Egg and Rice Waste as Green Supercapacitor
Authors: Qin Shumeng , Liu Peiliang , Wang Jieni , Liu Chenxiao , Zhang Shuqin , Tian Yijun , Zhang Fangfang , Wang Lin , Cao Leichang , Zhang Jinglai , Zhang Shicheng
Published: September 2023

The conversion of nitrogen–oxygen-rich biomass wastes into heteroatomic co-doped nanostructured carbons used as energy storage materials has received widespread attention. In this study, an in situ nitrogen–oxygen co-doped porous carbon was prepared for supercapacitor applications via a two-step method of pre-carbonization and pyrolytic activation using mixed egg yolk/white and rice waste. The optimal sample (YPAC-1) was found to have a 3D honeycomb structure composed of abundant micropores and mesopores with a high specific surface area of 1572.1 m2 g−1, which provided abundant storage space and a wide transport path for electrolyte ions. Notably, the specific capacitance of the constructed three-electrode system was as high as 446.22 F g−1 at a current density of 1 A g−1 and remained above 50% at 10 A g−1. The capacitance retention was 82.26% after up to 10,000 cycles. The symmetrical capacitor based on YPAC-1 with a two-electrode structure exhibited an energy density of 8.3 Wh kg−1 when the power density was 136 W kg−1. These results indicate that porous carbon materials prepared from mixed protein and carbohydrate waste have promising applications in the field of supercapacitors.
Ordered Mesoporous Carbons with Well-Dispersed Nickel or Platinum Nanoparticles for Room Temperature Hydrogen Adsorption
Authors: Szczęśniak Barbara , Głowniak Sylwia , Woźniak Jakub , Popiel Stanisław , Choma Jerzy , Jaroniec Mietek
Published: September 2023

A facile mechanochemical method was used for the synthesis of ordered mesoporous carbons (OMCs) with well-dispersed metal nanoparticles. The one-pot ball milling of tannins with a metal salt in the presence of a block copolymer followed by thermal treatment led to Ni- or Pt-embedded OMCs with high specific surface areas (up to 600 m2·g−1) and large pore volumes (up to ~0.5 cm3·g−1). The as-prepared OMC-based samples exhibited hexagonally ordered cylindrical mesopores with narrow pore size distributions (average pore size ~7 nm), which implies sufficient long-range copolymer-assisted self-assembly of the tannin-derived polymer upon milling even in the presence of a metal salt. The homogenous decoration of carbons with small-sized metal (Ni or Pt) particles was essential to provide H2 storage capacities up to 0.33 wt.% at 25 °C and under 100 bar. The presented synthesis strategy seems to have great potential in the practical uses of functionalized polymers and carbons for applications in adsorption and catalysis.
Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen
Authors: Zhang Huihua , Zhang Chaoyan , Qu Hui , Xi Fengna
Published: September 2023

Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is of great significance in cancer screening, early diagnosis, and postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through in situ growth of platinum nanoparticles (PtNPs) in a nanochannel array, which can achieve highly sensitive detection of the tumor marker carcinoembryonic antigen (CEA). An inexpensive and readily available indium tin oxide (ITO) glass electrode was used as the supporting electrode, and a layer of amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) was grown on its surface using an electrochemically assisted self-assembly method (EASA). The amino groups within the nanochannels served as anchoring sites for the one-step electrodeposition of PtNPs, taking advantage of the confinement effect of the ultrasmall nanochannels. After the amino groups on the outer surface of NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies were covalently immobilized followed by blocking nonspecific binding sites to create an immunorecognition interface. The PtNPs, acting as nanocatalysts, catalyzed the generation of reactive oxygen species (ROS) with hydrogen peroxide (H2O2), significantly enhancing the ECL signal of the luminol. The ECL signal exhibited high stability during continuous electrochemical scanning. When the CEA specifically bound to the immunorecognition interface, the resulting immune complexes restricted the diffusion of the ECL emitters and co-reactants towards the electrode, leading to a reduction in the ECL signal. Based on this immune recognition-induced signal-gating effect, the immunosensor enabled ECL detection of CEA with a linear range of 0.1 pg mL−1 to 1000 ng mL−1 with a low limit of detection (LOD, 0.03 pg mL−1). The constructed immunosensor demonstrated excellent selectivity and can achieve CEA detection in serum.
Challenges in Virtual Reality Training for CBRN Events
Authors: Regal Georg , Pretolesi Daniele , Schrom-Feiertag Helmut , Puthenkalam Jaison , Migliorini Massimo , De Maio Elios , Scarrone Francesca , Nadalin Marina , Guarneri Massimiliano , Xerri Grace P. , Di Giovanni Daniele , Tessari Paola , Genna Federica , D’Angelo Andrea , Murtinger Markus
Published: September 2023

The contemporary geopolitical environment and strategic uncertainty shaped by asymmetric and hybrid threats urge the future development of hands-on training in realistic environments. Training in immersive, virtual environments is a promising approach. Immersive training can support training for contexts that are otherwise hard to access, dangerous, or have high costs. This paper discusses the challenges for virtual reality training in the CBRN (chemical, biological, radioactive, nuclear) domain. Based on initial considerations and a literature review, we conducted a survey and three workshops to gather requirements for CBRN training in virtual environments. We structured the gathered insights into four overarching themes—the future of CBRN training, ethical and safety requirements, evaluation and feedback, and tangible objects and tools. We provide insights on these four themes and discuss recommendations.
Revisited Catalytic Hydrogen Evolution Reaction Mechanism of MoS2
Authors: He Yuhao , Chen Xiangpeng , Lei Yunchao , Liu Yongqi , Wang Longlu
Published: September 2023

MoS2 has long been considered a promising catalyst for hydrogen production. At present, there are many strategies to further improve its catalytic performance, such as edge engineering, defect engineering, phase engineering, and so on. However, at present, there is still a great deal of controversy about the mechanism of MoS2 catalytic hydrogen production. For example, it is generally believed that the base plane of MoS2 is inert; however, it has been reported that the inert base plane can undergo a transient phase transition in the catalytic process to play the catalytic role, which is contrary to the common understanding that the catalytic activity only occurs at the edge. Therefore, it is necessary to further understand the mechanism of MoS2 catalytic hydrogen production. In this article, we summarized the latest research progress on the catalytic hydrogen production of MoS2, which is of great significance for revisiting the mechanism of MoS2 catalytic hydrogen production.
Dual-Functional Tunable Metasurface for Meta-Axicon with a Variable Depth of Focus and Continuous-Zoom Metalens
Authors: Wang Chang , Sun Yan , Yu Zeqing , Liu Xinyu , Chen Bingliang , Zhang Yang , Zheng Zhenrong
Published: September 2023

Optical metasurfaces have been widely investigated for their versatile ability to manipulate wavefront and miniaturize traditional optical components into ultrathin planar devices. The integration of metasurfaces with multifunctionality and tunability has fundamentally transformed optics with unprecedented control over light propagation and manipulation. This study introduces a pioneering framework for the development of tunable metasurfaces with multifunctionality, and an example of a tunable metasurface of dual functionalities is proposed and numerically verified as one of the tunable meta-axicon for generating Bessel beams with a variable depth of focus (DOF) and a continuous-zoom metalens. Specifically, this design achieves dual-functional phase modulation by helicity-multiplexing from the combination of the geometric phase as well as the propagation phase and realizes tunability for both functionalities through rotational actuation between double metasurface layers. As a result, dual functionalities with continuous tunability of the proposed TiO2 metasurface are enabled independently for the left and right circularly polarized (LCP and RCP) incidences at 532 nm. Specifically, LCP light triggers the metasurface to function as a tunable axicon, generating non-diffracting Bessel beams with variable numerical apertures (NA) and DOFs. Conversely, the RCP incidence induces it to operate as a continuous-zoom metalens and generates variable spherical wavefront focusing on diverse focal lengths. This study not only initially implements the design of tunable meta-axicon, but also achieves the integration of such a tunable meta-axicon and continuous-zoom metalens within a single metasurface configuration. The proposed device could find potential applications in biological imaging, microscopic measurement, laser fabrication, optical manipulation, multi-plane imaging, depth estimation, optical data storage, etc.
Effect of Laser-Textured Cu Foil with Deep Ablation on Si Anode Performance in Li-Ion Batteries
Authors: Wang Jingbo , Cao Li , Li Songyuan , Xu Jiejie , Xiao Rongshi , Huang Ting
Published: September 2023

Si is a highly promising anode material due to its superior theoretical capacity of up to 3579 mAh/g. However, it is worth noting that Si anodes experience significant volume expansion (>300%) during charging and discharging. Due to the weak adhesion between the anode coating and the smooth Cu foil current collector, the volume-expanded Si anode easily peels off, thus damaging anode cycling performance. In the present study, a femtosecond laser with a wavelength of 515 nm is used to texture Cu foils with a hierarchical microstructure and nanostructure. The peeling and cracking phenomenon in the Si anode are successfully reduced, demonstrating that volume expansion is effectively mitigated, which is attributed to the high specific surface area of the nanostructure and the protection of the deep-ablated microgrooves. Moreover, the hierarchical structure reduces interfacial resistance to promote electron transfer. The Si anode achieves improved cycling stability and rate capability, and the influence of structural features on the aforementioned performance is studied. The Si anode on the 20 μm-thick Cu current collector with a groove density of 75% and a depth of 15 μm exhibits a capacity of 1182 mAh/g after 300 cycles at 1 C and shows a high-rate capacity of 684 mAh/g at 3 C.
The Circadian Nobiletin-ROR Axis Suppresses Adipogenic Differentiation and IκBα/NF-κB Signaling in Adipocytes
Authors: Kim Eunju , Mawatari Kazuaki , Yoo Seung-Hee , Chen Zheng
Published: September 2023

Obesity is a known risk factor for metabolic diseases and is often associated with chronic inflammation in adipose tissue. We previously identified the polyethoxylated flavonoid Nobiletin (NOB) as a circadian clock modulator that directly binds to and activates the ROR receptors in the core oscillator, markedly improving metabolic fitness in obese mice. Here, we show that NOB enhanced the oscillation of core clock genes in differentiated 3T3-L1 adipocytes, including ROR target genes such as Bmal1, Cry1, Dec1, and Dec2. NOB inhibited lipid accumulation in 3T3-L1 and SVF cells, concomitant with the dysregulated circadian expression of adipogenic differentiation-related genes including Cebpb, Pparg, Lpl, Scd1, and Fas. Importantly, RORα/RORγ double knockdown in 3T3-L1 cells (Ror DKD) significantly attenuated the effects of NOB on circadian gene expression and lipid accumulation. Furthermore, whereas NOB upregulated the expression of IκBα, a target of RORs, to inhibit NF-κB activation and proinflammatory cytokine expression, Ror DKD cells exhibited a heightened activation of the NF-κB pathway, further indicating a requisite role of RORs for NOB efficacy in adipocytes. Together, these results highlight a significant regulatory function of the NOB–ROR axis in the circadian expression of clock and clock-controlled genes in adipocytes, thereby governing adipogenic differentiation, lipogenesis, and inflammation.
Nutritional Ultrasonography, a Method to Evaluate Muscle Mass and Quality in Morphofunctional Assessment of Disease Related Malnutrition
Authors: López-Gómez Juan José , García-Beneitez David , Jiménez-Sahagún Rebeca , Izaola-Jauregui Olatz , Primo-Martín David , Ramos-Bachiller Beatriz , Gómez-Hoyos Emilia , Delgado-García Esther , Pérez-López Paloma , De Luis-Román Daniel A.
Published: September 2023

Nutritional ultrasonography is an emerging technique for measuring muscle mass and quality. The study aimed to evaluate the relationship between the parameters of body mass and quality of ultrasonography with other parameters of morphofunctional assessment in patients with disease-related malnutrition (DRM). Methods: A cross-sectional study was developed on 144 patients diagnosed with DRM according to the Global Leadership Initiative on Malnutrition (GLIM) criteria. Morphofunctional evaluation was assessed with anthropometric variables, handgrip strength and bioelectrical impedanciometry (BIA). Nutritional ultrasonography of quadriceps rectus femoris (QRF) was made (muscle mass (Muscle Area of Rectus Femoris index (MARFI)), Y axis and muscle quality (X-Y index and echogenicity). Results: The mean age of patients was 61.4 (17.34) years. The prevalence of sarcopenia in the sample was 33.3%. Patients with sarcopenia (S) had lower values of MARFI [(S: 1.09 (0.39) cm2/m2; NoS: 1.27 (0.45); p = 0.02), Y axis (S: 0.88 (0.27); NoS: 1.19 (0.60); p < 0.01) and X-Y index (S: 1.52 (0.61); NoS: 1.30 (0.53); p < 0.01)]. There was a correlation between BIA parameters (phase angle) and muscle mass ultrasonographic variables (MARFI) (r = 0.35; p < 0.01); there was an inverse correlation between muscle quality ultrasonographic variables (echogenicity) and handgrip strength (r = −0.36; p < 0.01). In the multivariate analysis adjusted by age, the highest quartile of the X-Y index had more risk of death OR: 4.54 CI95% (1.11–18.47). Conclusions: In patients with DRM and sarcopenia, standardized muscle mass and muscle quality parameters determined by ultrasonography of QRF are worse than in patients without sarcopenia. Muscle quality parameters had an inverse correlation with electric parameters from BIA and muscle strength. The highest quartile of the X-Y index determined by ultrasonography was associated with increased mortality risk.
Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Genes in Enterobacterales, Pseudomonas and Acinetobacter Isolates from the Uterus of Healthy Mares
Authors: Thomson Pamela , García Patricia , Río Camila del , Castro Rodrigo , Núñez Andrea , Miranda Carolina
Published: September 2023

Antibiotic-resistant bacteria are a growing concern for human and animal health. The objective of this study was to determine the antimicrobial resistance and extended-spectrum beta-lactamase genes in Enterobacterales, Pseudomonas spp. and Acinetobacter spp. isolates from the uterus of healthy mares. For this purpose, 21 mares were swabbed for samples, which were later seeded on blood agar and MacConkey agar. The isolates were identified using MALDI-TOF and the antimicrobial susceptibility test was performed using the Kirby–Bauer technique. To characterize the resistance genes, a polymerase chain reaction (PCR) scheme was performed. Of the isolates identified as Gram-negative, 68.8% were Enterobacterales, represented by E. coli, Enterobacter cloacae, Citrobacter spp., and Klebsiella pneumoniae; 28.1% belonged to the genus Acinetobacter spp.; and 3.1% to Pseudomonas aeruginosa. A 9.3% of the isolates were multidrug-resistant (MDR), presenting resistance to antibiotics from three different classes, while 18.8% presented resistance to two or more classes of different antibiotics. The diversity of three genes that code for ESBL (blaTEM, blaCTX-M and blaSHV) was detected in 12.5% of the strains. The most frequent was blaSHV, while blaTEM and blaCTX-M were present in Citrobacter spp. and Klebsiella pneumoniae. These results are an alarm call for veterinarians and their environment and suggest taking measures to prevent the spread of these microorganisms.
Synthesis and Evaluation of 99mTc-Labelled 2-Nitroimidazole Derivatives with Different Linkers for Tumour Hypoxia Imaging
Authors: Ruan Qing , Liu Yitong , Liao Lihao , Hao Jinyu , Jiang Yuhao , Jiang Jianyong , Zhang Junbo
Published: September 2023

When developing novel radiopharmaceuticals, a linker moiety between the chelator and targeting vector can have a crucial influence on adjusting the affinity of the tracer and its biodistribution in organisms. To develop novel 99mTc-labelled hypoxia imaging radiotracers, in this study, five isocyanide-containing 2-nitroimidazole derivatives with different linkers (L1, L2, L3, L4 and L5) were synthesised and radiolabelled with technetium-99m to obtain five stable 99mTc-complexes ([99mTc]Tc-L1, [99mTc]Tc-L2, [99mTc]Tc-L3, [99mTc]Tc-L4 and [99mTc]Tc-L5). Corresponding rhenium analogues of [99mTc]Tc-L1 were synthesised and suggested the structures of these 99mTc-complexes would be a monovalent cation with a technetium (I) core surrounded by six ligands. [99mTc]Tc-L1 is hydrophilic, while the lipophilicities of [99mTc]Tc-L2, [99mTc]Tc-L3, [99mTc]Tc-L4 and [99mTc]Tc-L5 are close. In vitro cell experiments showed that all five novel 99mTc-complexes had higher uptake in hypoxic cells compared with aerobic cells, which indicates the complexes have good hypoxia selectivity. The biodistribution of the five 99mTc-complexes in S180 tumour-bearing mice showed that they all had certain uptake in the tumours. Among them, [99mTc]Tc-L1 had the highest tumour-to-muscle (4.68 ± 0.44) and tumour-to-blood (3.81 ± 0.46) ratios. The introduction of polyethylene glycol (PEG) chains effectively reduced the lipophilicity and decreased uptake by the liver, intestine and blood but also increased clearance from the tumours. In vivo metabolic studies showed [99mTc]Tc-L1 kept intact and remained stable in tumour, blood and urine at 2 h post-injection. The results of SPECT imaging showed that [99mTc]Tc-L1 had significant tumour uptake at 2 h post-injection, but there was still high uptake in abdominal organs such as the liver and kidney, suggesting that this complex needs to be further optimised before being used for tumour hypoxia imaging.
MedSMA℞T Adventures in PharmaCity Game: Youth Experiences and Recommendation for Use in Opioid Safety Education
Authors: Abraham Olufunmilola , Koeberl Courtney R. , McCarthy Tyler J.
Published: September 2023

Adolescents are often excluded from the creation of opioid safety interventions; therefore, it is crucial to design evidence-based interventions tailored for and with youth. Video games are ubiquitous and approachable to adolescents making them an accessible educational modality. MedSMA℞T: Adventures in PharmaCity is a serious game that educates adolescents and their families on the safe, appropriate, and responsible use of opioid prescriptions. The first objective of the study was to elucidate adolescents’ experiences and perceptions of the game. The second objective was to elicit their recommendations for use and suggestions for improvement. Adolescents were recruited through Qualtrics research panels, social media, listservs, and snowball sampling. Recruitment occurred between April 2021 and October 2021. Eligible adolescents played the game and completed a follow-up virtual semi-structured interview with a study team member. Interviews were transcribed verbatim and uploaded to NVivo for data analysis. A thematic content analysis was performed. A total of seventy-two adolescents participated. Analysis yielded four themes: prior gaming experience, educational salience, game design impressions, and recommendations for improvement. Most adolescents approached MedSMA℞T with prior gaming experience. The youth correctly identified the game’s intended objective: the promotion of opioid medication safety. Adolescents had overarchingly positive impressions of the game’s levels, characters, and graphics. Study participants suggested expanded game levels, improved controls, and more instructions for gameplay. In summary, adolescents had favorable experiences using the MedSMA℞T game which allude to the wide-spread acceptability of this intervention among young people.
Spektr–UF Mission Spectrograph Space Qualified CCD Detector Subsystem
Authors: Shugarov Andrey , Sachkov Mikhail
Published: September 2023

Spektr–UF (World Space Observatory Ultraviolet, WSO-UV) is a Russian-led international collaboration aiming to develop a large space-borne 1.7 m Ritchey–Chretién telescope with science instruments to study the Universe in ultraviolet wavelengths. The WSO-UV spectrograph (WUVS) consists of three channels: two high-resolution channels (R = 50,000) with spectral ranges of 115–176 nm and 174–310 nm, and a low-resolution (R = 1000) channel with a spectral range of 115–305 nm. Each of the three channels has an almost identical custom detector consisting of a CCD inside a vacuum enclosure, and drive electronics. The main challenges of the WUVS detectors are to achieve high quantum efficiency in the FUV-NUV range, to provide low readout noise (3 e− at 50 kHz) and low dark current (<12 e−/pixel/hour), to operate with integral exposures of up to 10 h and to provide good photometric accuracy. A custom vacuum enclosure and three variants of a custom CCD272-64 sensor with different UV AR coatings optimised for each WUVS channel were designed. The enclosure prevents contamination and maintains the CCD at the operating temperature of −100 ∘C, while the temperature of the WUVS optical bench is +20 ∘C. A camera electronics box (CEB) that houses the CCD drive electronics was developed. Digital correlated double sampling technology allows for extremely low readout noise and flexible frequency for normal and binned pixel readout modes. This paper presents the WUVS detector design drivers, methods for extending the service life of the CCD sensors working with low signals in a space radiation environment and the key calculated parameters and results of the engineering qualification model qualification campaign.
Evaluation of the Chemical Profile and Antioxidant Capacity of Green, Brown, and Dark Propolis
Authors: Vieira Ana Luiza Santos , Correia Vinícius Tadeu da Veiga , Ramos Ana Luiza Coeli Cruz , da Silva Nayana Hayss Araújo , Jaymes Leonardo Assis Campos , Melo Julio Onésio Ferreira , de Paula Ana Cardoso Clemente Filha Ferreira , Garcia Maria Aparecida Vieira Teixeira , Araújo Raquel Linhares Bello de
Published: September 2023

The chemical composition of propolis varies between different types, due to the specific vegetation found near the hives and the climatic and soil conditions worldwide. Green propolis is exclusive to Brazil, produced by bees, with the resin of the plant Baccharis dracunculifolia. Brown propolis is a specific variety produced mainly in Northeast Brazil from the plant Hyptis divaricata, also known as “maria miraculosa”. Dark propolis is a variety of propolis produced by bees from the resin of the plant known as Jurema Preta (Mimosa hostilis benth). In this study, the aqueous extracts of green, brown, and dark propolis were analyzed for their antioxidant capacity using ABTS, FRAP, and DPPH, and their chemical profiles were determined using paper spray mass spectrometry. Among the three extracts, green propolis had the highest content of total phenolic compounds (2741.71 ± 49.53 mg GAE. 100 g−1), followed by brown propolis (1191.55 ± 36.79 mg GAE. 100 g−1), and dark propolis had the lowest content (901.79 ± 27.80 mg GAE. 100 g−1). The three types of propolis showed high antioxidant capacity, with green showing the highest antioxidant capacity for the three methods used. Using paper spray mass spectrometry, it was possible to suggest the presence of 116 substances, including flavonoids (56), phenylpropanoids (30), terpenes (25), carboxylic acids (1), benzoic acid derivatives (1), fatty acids (1), amino acids (1) and alkaloids (1). The compounds in the green, brown, and dark propolis extracts reinforce the bioactive potential for application in these tree extracts’ food and pharmaceutical products.
Pitaya as a New Alternative Crop for Iberian Peninsula: Biology and Edaphoclimatic Requirements
Authors: Trindade Ana Rita , Paiva Paulo , Lacerda Vander , Marques Natália , Neto Luís , Duarte Amílcar
Published: September 2023

Pitaya is one of the fruit species whose demand has increased in recent years due to the numerous health benefits and lucrative price of the fruit and its by-products. In Europe, the Iberian Peninsula and other Mediterranean countries are the ones with favorable climatic conditions for its cultivation. This document describes much of the history of pitaya in the Iberian Peninsula and the difficulties related to its cultivation. A bibliographical survey was carried out on the culture of pitaya in the world, focusing on the edaphoclimatic requirements, and on the possibility of this becoming a consolidated crop in the Iberian Peninsula. The relatively low water requirement of pitaya makes this crop sustainable among crops that require irrigation. In addition, we provide a perspective for use and research of this emerging crop. There has been an exponential growth of scientific publications on pitaya in the last decade; however, much more needs to be researched to know how to increase productivity as well as the sensory quality of fruits in different regions. This sustainable crop is a good option to diversify fruit production in the Iberian Peninsula.
Chitosanase Production from the Liquid Fermentation of Squid Pens Waste by Paenibacillus elgii
Authors: Doan Chien Thang , Tran Thi Ngoc , Tran Thi Phuong Hanh , Nguyen Thi Thanh , Nguyen Huu Kien , Tran Thi Kim Thi , Vu Bich Thuy , Trinh Thi Huyen Trang , Nguyen Anh Dzung , Wang San-Lang
Published: September 2023

Chitosanases play a significant part in the hydrolysis of chitosan to form chitooligosaccharides (COS) that possess diverse biological activities. This study aimed to enhance the productivity of Paenibacillus elgii TKU051 chitosanase by fermentation from chitinous fishery wastes. The ideal parameters for achieving maximum chitosanase activity were determined: a squid pens powder amount of 5.278% (w/v), an initial pH value of 8.93, an incubation temperature of 38 °C, and an incubation duration of 5.73 days. The resulting chitosanase activity of the culture medium was 2.023 U/mL. A chitosanase with a molecular weight of 25 kDa was isolated from the culture medium of P. elgii TKU051 and was biochemically characterized. Liquid chromatography with tandem mass spectrometry analysis revealed that P. elgii TKU051 chitosanase exhibited a maximum amino acid identity of 43% with a chitosanase of Bacillus circulans belonging to the glycoside hydrolase (GH) family 46. P. elgii TKU051 chitosanase demonstrated optimal activity at pH 5.5 while displaying remarkable stability within the pH range of 5.0 to 9.0. The enzyme displayed maximum efficiency at 60 °C and demonstrated considerable stability at temperatures ≤40 °C. The presence of Mn2+ positively affected the activity of the enzyme, while the presence of Cu2+ had a negative effect. Thin-layer chromatography analysis demonstrated that P. elgii TKU051 chitosanase exhibited an endo-type cleavage pattern and hydrolyzed chitosan with 98% degree of deacetylation to yield (GlcN)2 and (GlcN)3. The enzymatic properties of P. elgii TKU051 chitosanase render it a promising candidate for application in the production of COS.
Acetylcellulose Recovery from Waste Residual for Attenuating Reactive Dye from Aquaculture Waste as a Fascinating Synergistic Ecology Effect
Authors: Nabwey Hossam A. , Tony Maha A. , Nour Manasik M.
Published: September 2023

Waste valorization is attracting not only the scientific world but also the world. Acetylcellulose wastes from cigarette filter residuals are signified as the largest global impact of solid waste. Acetylcellulose recycling for desired products is a promising way for environmental management. In this regard, the current investigation is dealing with the immersion of residual filters sequentially into aqueous solutions of alkali and ethanol before water washing, which converts them into a superhydrophobic acetylcellulose adsorbent material. The morphology and characteristics of the acetylcellulose fiber were characterized using a Scanning Electron Microscope (SEM) and Fourier-Transform infrared spectroscopy (FTIR). The adsorption tendency was checked for Levafix Blue dye compromised in an aqueous stream as a model textile polluted effluent. The experimental results exposed that the acetylcellulose fiber displayed a sensible textile dye elimination from the dying stream. Langmuir isotherm is well fitting the adsorption matrix and the reaction follows the 1st-order kinetic model. The so-obtained acetylcellulose fiber showed tremendous efficiency for dye removal from aqueous effluent. The attained maximum monolayer adsorption capacity was recorded as 4.8 mg/g at pH 3.0 and an adsorbent dose of 1 g/L through the isotherm time of 2 h. Also, temperature elevation could increase the adsorption capacity to 5.7 mg/g. Due to this excellent affinity to adsorb dye at an economic wise rate is shown as a promising candidate for textile dye elimination form aqueous effluent.
Artificial Intelligence and Carbon Emissions in Manufacturing Firms: The Moderating Role of Green Innovation
Authors: Chen Yixuan , Jin Shanyue
Published: September 2023

Carbon emissions have gained worldwide attention in the industrial era. As a key carbon-emitting industry, achieving net-zero carbon emissions in the manufacturing sector is vital to mitigating the negative effects of climate change and achieving sustainable development. The rise of intelligent technologies has driven industrial structural transformations that may help achieve carbon reduction. Artificial intelligence (AI) technology is an important part of digitalization, providing new technological tools and directions for the low carbon development of enterprises. This study selects Chinese A-share listed companies in the manufacturing industry from 2012 to 2021 as the research objects and uses a fixed-effects regression model to study the relationship between AI and carbon emissions. This study clarifies the significance of enterprise AI technology applications in realizing carbon emissions reduction and explores the regulatory mechanism from the perspective of the innovation effect. The results show that the application of enterprise AI technology positively impacts carbon emissions reduction. Simultaneously, green technological innovation, green management innovation, and green product innovation play moderating roles; in other words, enterprise green innovation strengthens the effect of AI on carbon emissions reduction. This study clarifies the necessity of intelligent manufacturing and enriches theories related to AI technology and carbon emissions.
A Tabu-Matching Heuristic Algorithm Based on Temperature Feasibility for Efficient Synthesis of Heat Exchanger Networks
Authors: Huang Xiaohuang , Shen Hao , Yue Wenhao , Duan Huanhuan , Cui Guomin
Published: September 2023

The non-structural model of heat exchanger networks (HENs) offers a wide solution space for optimization due to the random matching of hot and cold streams. However, this stochastic matching can sometimes result in infeasible structures, leading to inefficient optimization. To address this issue, a tabu matching based on a heuristic algorithm for HENs is proposed. The proposed tabu-matching method involves three main steps: First, the critical temperature levels—high, medium, and low-temperature intervals—are determined based on the inlet and outlet temperatures of streams. Second, the number of nodes is set according to the temperature intervals. Third, the nodes of streams are flexibly matched within the tabu rules: the low-temperature interval of hot streams with the high-temperature interval of cold streams; the streams crossing cannot be matched. The results revealed that by incorporating the tabu rules and adjusting the number of nodes, the ratio of the feasible zone in the whole solution domain increases, and the calculation efficiency is enhanced. To evaluate the effectiveness of the method, three benchmark problems were studied. The obtained total annual costs (TACs) of these case studies exhibited a decrease of USD 4290/yr (case 1), USD 1435/yr (case 2), and USD 11,232/yr (case 3) compared to the best published results. The results demonstrate that the proposed tabu-matching heuristic algorithm is effective and robust.