bluuebunny/crossref_metadata_2025 huggingface dataset:
HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE
Authors: Ratkin A. V. , Pfarger Yu. A. , Ivanov V. V. , Adekenov S. M. , Kaidash O. A. , Chuchalin V. S. , Novitsky V. V.
Published: June 2020

Objective.Investigation of hypolipidemic effect of sesquiterpene γ-lactone Arglabin in hepatoma tissue culture (HTC).

Materials and methods.In this study we’ve evaluated the effect of sesquiterpene γ-lactone Arglabin and gemfibrozil (reference drug) on the lipid content in the hepatoma tissue culture (HTC) which were incubated with a fat emulsion “Lipofundin” by fluorescent method with vital dye Nile Red. The cell viability was investigated using the MTT-test and staining by Trypan blue.

Results.Cultivation of cell cultures of rat’s hepatoma cell line HTC with Arglabin and gemfibrozil in concentrations from 10 to 50 μmol and from 0.25 to 0.5 mmol, respectively, had no cytotoxic effect. HTC cell viability did not change compared with the corresponding rate in the control culture. Experimental hyperlipidemia in hepatoma culture was induced by the addition in the incubation medium of fat emulsion “Lipofundin” in a final concentration of 0.05 %. The fluorescence intensity of Nile Red in the cells was increased 4-fold (p < 0.05), which indicates a significant accumulation of lipids in the cytosol of cells. In these steady-state Arglabin and gemfibrozil at concentrations 75–100 μM and 0.25–1.0 mM, respectively, reduced the content of lipid in cells. 

Conclusion.In the model of hyperlipidemia induced by lipofundin, sesquiterpene γ-lactone Arglabin prevents the accumulation of lipids in the HTC cell line, as evidenced by a decrease in Nile Red fluorescence. However hypolipidemic effect of Arglabin is associated with cytotoxic effects, which is typical for anticancer drugs.
EXPRESSION OF IGFBP-6 IN COLORECTAL CANCER: THE RELATION WITH INSULIN-LIKE GROWTH FACTOR RECEPTOR, ADIPONECTIN LEVEL AND ITS RECEPTORS
Authors: Yunusova N. V. , Spirina L. V. , Afanasiev S. G. , Kondakova I. V.
Published: June 2020

The aim of the study was to investigate the expression of insulin-like growth factor binding protein 6 (IGFBP-6) in cancer tissues in relation with clinical and morphological parameters, IGF-IR expression, serum adiponectin level and its receptors (AdipoR1, AdipoR2) in patients with colorectal cancer.

Material and Methods. The study included 31 patients with colorectal cancer (T2–4N0–2M0). Serum adiponectin level, AdipoR1 and AdipoR2 expression were evaluated with ELISA. IGF-IR expression was evaluated in tumor tissue by flow cytometry. IGFBP-6 expression was evaluated with Western blotting.

Results. The dependence of IGFBP-6 expression, AdipoR1, AdipoR2 on tumor invasion and lymph nodes status were revealed. There is no association IGFBP-6 expression, AdipoR1 and AdipoR2 expression and serum adiponectin level with metabolic syndrome. The revealed positive relationships between IGFBP-6 expression and AdipoR1 expression, between IGFBP-6 expression and AdipoR2 expression, between IGF-IR and AdipoR1 expression show cross-talk between IGF-IR and adiponectin/AdipoR1 pathways in colorectal carcinomas.
Mécanismes et conséquences de la croissance compensatrice chez les ruminants
Authors: HOCH T. , BEGON C. , CASSAR-MALEK I. , PICARD B. , SAVARY-AUZELOUX I.
Published: June 2020

L’alternance, volontaire ou non, de périodes d’apports alimentaires réduits et élevés engendre chez les animaux un phénomène de croissance compensatrice. Bien que son étude ait fait l’objet de nombreux travaux, les avancées récentes des recherches méritent d’être synthétisées, ce qui fait l’objet du présent article. Plus particulièrement, l’influence de phases de restriction et de réalimentation sur la qualité finale des produits est un aspect novateur des recherches menées dans ce domaine. Par ailleurs, les mécanismes hormonaux liés à la compensation sont dorénavant mieux connus. Ils gouvernent l’orientation des métabolismes énergétique et protéique vers l’accroissement de l’accrétion protéique pendant la croissance compensatrice. L’influence de la compensation sur la composition corporelle finale des animaux est faible, même si certains auteurs décrivent des animaux plus maigres à la fin d’une phase de compensation. En ce qui concerne les caractéristiques des muscles, les changements induits par une restriction alimentaire sont souvent réversibles et annulés par la compensation. Néanmoins, certaines études montrent une amélioration de la qualité sensorielle finale de la viande, plus particulièrement de la tendreté. Ces résultats sont à nuancer, mais justifient l’intérêt porté aux études reliant croissance discontinue et caractéristiques du muscle et de la viande. Des développements futurs et des prolongements de ces recherches sont envisagés et mis en perspective.
La production laitière dans les montagnes françaises : une dynamique particulière pour les Alpes du Nord
Authors: CHATELLIER V. , DELATTRE F.
Published: June 2020

L’attachement du quota laitier au foncier, la gratuité des droits à produire et la redistribution des références laitières aux agriculteurs jugés prioritaires ont, en France, favorisé le maintien d’un nombre relativement important d’exploitations laitières en zone de montagne. A ces choix de politique publique s’ajoute le dynamisme des acteurs locaux et des filières de transformation (développement des fromages sous signes de qualité) qui a joué un rôle déterminant au cours des deux dernières décennies. En partant des données du réseau d’information comptable agricole (RICA) de l’exercice 2000, la situation structurelle, économique et financière des exploitations laitières françaises de montagne est comparée à celles des zones défavorisées simples et des zones de plaine, puis de façon déclinée pour différents massifs montagneux (Alpes du Nord, Jura, Massif Central et Vosges). Les 27 200 exploitations laitières ’professionnelles’ françaises de montagne (lesquelles représentent 35 % des exploitations agricoles et 40 % de la production agricole de ces zones) disposent, en moyenne, d’un quota de 150 800 kg de lait, de 61 hectares et de 22 400 euros de résultat courant avant impôt (contre respectivement 222 700 kg, 72 hectares et 31 500 euros en plaine). Une analyse centrée sur les dynamiques internes de la filière laitière des Alpes du Nord est ensuite présentée moyennant la valorisation de différents outils statistiques.
Production et conservation de la semence de bouc pour l’insémination artificielle
Authors: LEBOEUF B. , RESTALL B. , SALAMON S.
Published: June 2020

Chez les races caprines saisonnées, les méthodes d’amélioration de production et de conservation de semence doivent prendre en considération l’influence de l’environnement et en particulier de la photopériode. Par la fonction qu’elle exerce sur la régulation du développement gonadique, celle-ci contribue indirectement au contrôle du comportement sexuel et de la production de sperme, tant du point de vue quantitatif que qualitatif. L’utilisation de traitements photopériodiques constitue donc un outil efficace pour augmenter la production des doses de sperme destinées à l’insémination artificielle durant la carrière de reproducteur du bouc. Afin d’optimiser la production et la qualité de la semence, une sélection préalable des mâles est cependant nécessaire.
Un problème spécifique dans la conservation de la semence de bouc est l’effet délétère du plasma séminal sur la viabilité des spermatozoïdes après congélation / décongélation, principalement lorsqu’ils sont dilués dans des milieux à base de lait ou de jaune d’œuf. La protéine responsable de cet effet est une enzyme sécrétée par les glandes bulbo-uréthrales appartenant à la famille des lipases pancréatiques et qui possède une double activité lipasique et phospholipasique.
La conservation de la semence à l’état liquide, peu coûteuse, est un complément à la cryconservation pour développer l’insémination artificielle à grande échelle. Mais la diminution de la fécondance après 12 heures de conservation est un frein à l’utilisation de cette technique. Les méthodes de congélation disponibles actuellement diffèrent sur plusieurs aspects. Les plus efficaces incluent le lavage de la semence pour éliminer le plasma séminal dès la collecte.
Dossier : Typologie et ontogenèse des fibres musculaires chez différentes espèces d’intérêt agronomique
Authors: PICARD B. , JURIE C. , HOCQUETTE J.F. , LEFAUCHEUR L. , BERRI C. , DUCLOS M.J. , ALAMI-DURANTE H. , RESCAN P.Y.
Published: June 2020

Chez les espèces d’intérêt agronomique, les caractéristiques des fibres musculaires jouent un rôle important dans le déterminisme de la qualité organoleptique de la viande. Ce dossier fait le point des connaissances concernant la caractérisation des fibres musculaires et l’acquisition de leurs propriétés (contractiles et métaboliques) dans les principales espèces productrices de viande (bovins, porcs, volailles) et chez les poissons. La mise en place des fibres musculaires, qui débute très tôt au cours de la vie fœtale, implique des vagues successives de plusieurs générations de cellules myogéniques qui sont à l’origine des différents types de fibres. Le nombre total de fibres reste stable après la naissance chez le bovin, le porc et les volailles. En revanche, chez certains poissons, il augmente encore au cours de la vie postnatale. Chez le bovin, les principales étapes de la différenciation contractile et métabolique des fibres ont lieu durant le dernier tiers de la vie fœtale. En revanche, l’acquisition de ces propriétés se fait principalement pendant les deux semaines qui suivent la naissance chez le porc ou la volaille. Ainsi, si les principes généraux du développement musculaire sont comparables dans les différentes espèces, ils se déroulent selon une cinétique différente en relation avec la maturité de chaque espèce à la naissance. Toutefois, dans toutes les espèces, les propriétés des fibres sont caractérisées par une large plasticité tout au long de la vie des animaux, ce qui laisse la possibilité de les modifier par le biais des facteurs d’élevage.
Typologie et ontogenèse des fibres musculaires chez les poissons
Authors: ALAMI-DURANTE H. , RESCAN P.Y.
Published: June 2020

Cet article fait partie du Dossier Typologie et ontogenèse des fibres musculaires chez différentes espèces d’intérêt agronomique.
Computational experiment of error diffusion dithering for depth reduction in images
Authors: Rezende Costa Leonardo
Published: June 2020

The halftone technique is a process that employs patterns formed by black and white dots to reduce the number of gray levels in an image. Due to the tendency of the human visual system to soften the distinction between points with different shades, the patterns of black and white dots produce a visual effect as if the image were composed of shades of gray and dark. This technique is quite old and is widely used in printing images in newspapers and magazines, in which only black (ink) and white (paper) levels are needed. There are several methods for generating halftone images. In this article we explore dithering with error diffusion and an analysis of different halftone techniques is presented using error diffusion to change the depth of the image. The results showed that the depth of the image changes 1/8 per channel, this halftone technique can be used to reduce an image weight, losing information but achieving good results, depending on the context.

ontext.
Aos tambores, as margens: as casas de religiões de matrizes afro-brasileiras na microrregião de Cataguases
Authors: Cruz Inácio Manoel Neves Frade da
Published: June 2020

O presente texto tem como propósito oferecer uma visão panorâmica sobre as religiões de matrizes afro-brasileiras em quatro municípios pertencentes à microrregião de Cataguases. Pautado na noção de racismo epistemológico, difundida por Abdias do Nascimento, procurou-se ocupar uma lacuna no que se refere à produção do conhecimento sobre as crenças alternativas, sobretudo, às denominações evangélicas e católica. A possibilidade de desconstruir estereótipos e dar a conhecer alguns templos de umbanda e candomblé nas localidades estudadas, bem como a permanência dos casos de intolerância religiosa contra as suas lideranças e frequentadores, foram questões que motivaram esta pesquisa.
Análisis de la práctica educativa de los museos pedagógicos españoles mediante una experiencia de evaluación de iniciativas, actividades y recursos
Authors: Álvarez Domínguez Pablo , Rebollo Espinosa María José
Published: June 2020

Los museos pedagógicos españoles han venido desarrollando desde su fundación una serie de prácticas educativas que han contribuido de manera determinante (con sus luces y sus sombras) a que hoy sean lo que son. Han emprendido múltiples y diversas iniciativas y actividades para conservar, difundir y ayudar a interpretar el patrimonio educativo. Y de igual forma, cuentan con una gran variedad de recursos que les han permitido llevar a cabo tales funciones. Así, pues, tras una trayectoria educadora más o menos alargada en el tiempo, es el momento de hacer balance. Para responder a este objetivo, hemos utilizado la técnica de evaluación pedagógica Hashtag con un conjunto de miembros de la comunidad de historiadores/as de la educación, interesados especialmente en el estudio del patrimonio educativo, a fin de obtener datos que nos ayuden a esbozar el panorama de las fortalezas, debilidades y anhelos de estos Museos. Los resultados ponen de manifiesto, entre las prácticas consideradas como positivas y transferibles: su uso didáctico, su labor de recuperación y difusión del patrimonio educativo, la colaboración con los centros escolares o el empleo de metodologías alternativas para el aprendizaje de la Historia de la Educación con el alumnado universitario. Las debilidades más repetidas tienen que ver con la falta de personal, espacios y financiación. Y, como propósitos deseables, destacan la necesidad de establecer redes museísticas y planificar proyectos museográficos más interactivos, inclusivos y sociales.
Estimation of Flexible Pavement Structural Capacity Using Machine Learning Techniques
Authors: Karballaeezadeh Nader , Ghasemzadeh Tehrani Hosein , Mohammadzadeh S. Danial , Shamshirband Shahaboddin
Published: June 2020

The most common index for representing structural condition of the pavement is the structural number. The current procedure for determining structural numbers involves utilizing falling weight deflectometer and ground-penetrating radar tests, recording pavement surface deflections, and analyzing recorded deflections by back-calculation manners. This procedure has two drawbacks: 1. falling weight deflectometer and ground-penetrating radar are expensive tests, 2. back-calculation ways has some inherent shortcomings compared to exact methods as they adopt a trial and error approach. In this study, three machine learning methods entitled Gaussian process regression, m5p model tree, and random forest used for the prediction of structural numbers in flexible pavements. Dataset of this paper is related to 759 flexible pavement sections at Semnan and Khuzestan provinces in Iran and includes “structural number” as output and “surface deflections and surface temperature” as inputs. The accuracy of results was examined based on three criteria of R, MAE, and RMSE. Among the methods employed in this paper, random forest is the most accurate as it yields the best values for above criteria (R=0.841, MAE=0.592, and RMSE=0.760). The proposed method does not require to use ground penetrating radar test, which in turn reduce costs and work difficulty. Using machine learning methods instead of back-calculation improves the calculation process quality and accuracy.
Government Size dan Pertumbuhan Ekonomi di Indonesia
Authors: Santika Ary Ratna , Qibthiyyah Riatu Mariatul
Published: June 2020

The aim of this research to investigate the effect of government size on regional economic growth in Indonesia. This study uses panel data analysis with using two years of interval data from 2009 until 2015. The results showed that government size has a significant effect on economic growth and it has a non-linear relationship. According the estimation from the model, the threshold government size on regional economic growth is 38.98%. Above the threshold, an increase in government size will instead have a negative effect on economic growth. ------------------------------------------ Penelitian ini bertujuan untuk meneliti pengaruh government size terhadap pertumbuhan ekonomi daerah di Indonesia. Penelitian ini menggunakan analisis data panel dengan menggunakan data dua tahun interval dari tahun 2009 hingga 2015. Hasil penelitian menunjukkan bahwa government size berpengaruh signifikan terhadap pertumbuhan ekonomi daerah dan berpengaruh secara tidak linier. Berdasarkan hasil model, dapat dihitung bahwa threshold untuk government size terhadap pertumbuhan ekonomi daerah adalah sebesar 38,98%. Jika pertumbuhan ekonomi daerah lebih dari itu, peningkatan government size akan berdampak negatif pada pertumbuhan ekonomi.
Analisis Pengaruh Frekuensi Belajar di Luar Sekolah dan Motivasi Belajar terhadap Hasil Belajar IPA
Authors: Hartina Tika , Mahrus Mahrus , Hadiprayitno Gito
Published: June 2020

The purpose of this study is to determine the effect of frequency of out-school learning activities and learning motivation towards improving students' natural science learning achievement. This research is quantitative research with an ex-post facto approach. The subjects in the study were 8th grade students of SMP Negeri 8 Mataram in the academic year at 2018/2019 with a total of 260 people. Data collection of science learning outcomes is obtained from daily tests from teachers in schools while data collection of the frequency of out-school learning activities and learning motivation is obtained using a questionary. The effect of frequency of out-school learning activities and learning motivation on learning achievement of science are analyzed using analysis of covariance (ANAKOVA). The results of the study show that: 1) the frequency of out-school learningactivities has a significant influence on science learning achievement; 2) student's learning motivation has a significant effect on science learning achievement, and 3) there is an interaction between the frequency of out-school learning activities and learning motivation on students' learning achievement in science. The frequency of out-school learning activities and learning motivation is proven have effect on science learning achievement.
Sikap Siswa Terhadap IPA berdasarkan Investigasi dan Korelasi: Kesenangan Belajar dan Minat Meluangkan Waktu pada IPA
Authors: Kurniawan Dwi Agus , Astalini Astalini , Kurniawan Nugroho , Anggraini Lika
Published: June 2020

This study purposed to investigate attitudes towards science in junior high school students, based on 2 attitude indicators: (1) enjoyment in science lesson & (2) leisure interest in science. In addition, measurements were also made regarding the relationship between the two indicators of attitude. This research is survey research. The results of the data came from an attitude questionnaire consisting of indicators (1) enjoyment in science lesson and (2) leisure interest in science. The sample consisted of 2815 junior high school students in Jambi Province, Indonesia. The results showed that for indicators: (1) enjoyment in science is good category with a percentage of 48.1%, (2) leisure interest in science is fairly good category with a value: 47.2% and This indicator leads to a positive trend supported by a positive attitude with a greater good value: 38.4%, while for the analysis of the correlation between the two indicators shows a positive correlation with a sig: 0,000 (<0.05) and has a strong degree of correlation indicated by the value "Pearson Correlation": 0.716. Therefore, it is concluded overall attitudes towards science at Junior high school students in Jambi, Indonesia have a positive attitude and are categorized well.
Inbar Graiver, Asceticism of the Mind: Forms of Attention and Self-Transformation in Late Antique Monasticism. Studies and Texts 213, Toronto, Pontifical Institute of Mediaeval Studies, 2018, x + 238 pp., ISBN: 9780888442130. Cloth $80
Authors: WOOD JAMIE
Published: June 2020

Reseñado por JAMIE WOOD University of Lincoln, UK jwood@lincoln.ac.uk
Dragos Calma and Zenon Kaluza (eds.), Regards sur les traditions philosophiques (XIIe – XVIe siècles), Ancient and Medieval Philosophy 1, Leuven, Leuven University Press, 2017, vi + 341 pp., ISBN: 9789462701243. Cloth €85
Authors: HERRERA JUAN JOSÉ
Published: June 2020

Reseñado por JUAN JOSÉ HERRERA Universidad del Norte Santo Tomás de Aquino San Miguel de Tucumán, AR jjherreraop@hotmail.com
Fray Luis de León, Cuestiones sobre la Encarnación. Introducción, transcripción, versión y notas de José Manuel Díaz Martín, Salamanca, Ediciones Universidad de Salamanca, 2018, 172 pp., ISBN: 9788490128497. Cloth €14
Authors: CASTILLA FRANCISCO
Published: June 2020

Reseñado por FRANCISCO CASTILLA URBANOUniversidad de Alcalá, ESfrancisco.castilla@uah.es
María Asunción Sánchez Manzano, El escepticismo humanista de Francisco Sánchez, Madrid, Dykinson, 2018, 145 pp., ISBN: 9788491489795. Cloth €17
Authors: ORTIZ CARLOS
Published: June 2020

Reseñado por CARLOS ORTIZ DE LANDÁZURIUniversidad de Navarra, Pamplona, EScortiz@unav.es
Basement Flood Control with Adaptive Neuro Fuzzy Inference System Using Ultrasonic Sensor
Authors: Kusumah Raden Muhamad Yuda Pradana , Abdurohman Maman , Putrada Aji Gautama
Published: June 2020

This paper proposes a basement flood management system based on Adaptive Neuro Fuzzy Inference System (ANFIS). Basement is one of the main parts of a building that has a high potential for flooding. Therefore, the existence of a flood control system in the basement can be a solution to this threat. Water level control is the key to solving the problem. Fuzzy Inference System (FIS) has proven to be a reliable method in the control system but this method has limitations, that is, it needs to have a basis or a reference when determining the fuzzy set. When there is no basis or reference, Adaptive Neuro FIS (ANFIS) can be a solution. The Neuron aspect in ANFIS determines fuzzy sets through training data. In terms of the Internet of Things (IoT), this system uses an ultrasonic sensor, Node Red IoT platform, and Matlab Server.  Then the water pump will turn on to control the water level when there is rainfall. By undergoing a comparative test with the FIS method, ANFIS provides a lower Root Mean Square Error (RMSE) and is recommended for use in basement flood management systems.
Effect of gases released during solidification of castings on microstructure of alloys
Authors: Marukovich E. I. , Stetsenko V. Yu.
Published: June 2020

Thermodynamic calculation of formation of spherical gas bubbles different in water – wetting on flat surfaces by metal melt is made. This process consists of two stages. The first stage is formation of equilibrium gas bubble in the form of ball segment. The second stage is its swinging into a spherical bubble. Calculations were made for gas bubbles with constant volume. It is shown that Gibbs energy of spherical gas bubble formation on water – wetting metal melt substrate is three times less than on nonwettable melt substrate. Thus, gases released by solidification of the castings will preferably be formed and removed as bubbles on the molten metal surfaces of the crystallizable phases of the alloys and directly affect on their microstructure.
Influence of the quality of compounds on the adhesion properties of metal cord.
Authors: Khrol O. N.
Published: June 2020

The adhesive strength of the metal cord, i. e. the strength of its adhesion to the compound, is one of the most important indicators of the quality of the metal cord. Increasing the amount of adhesive strength allow to improve the performance of finished tires.

Determination of the adhesive strength value can be performed by different methods. All methods are based on extracting (pulling) metal cord from a block of vulcanized rubber and fixing the required load. Each method has its own characteristics that affect the amount of adhesive strength. Therefore, when conducting comparative tests, it is necessary to use one of the selected methods.

The study of factors that affect the amount of adhesive strength is necessary to identify the causes that lead to its change.

The article analyzes how the quality of the used compound affects the adhesive strength. Tests with the use of compounds with various defects were considered and carried out; probable factors leading to deviations in the adhesion strength indicators were determined.
THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO
Authors: Asmilia Nuzul , TR T Armansyah , Aliza Dwinna , Melia Juli , Rahmi Erdiansyah , Daulay Lingga Surya Maret
Published: June 2020

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.
CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE
Authors: Adam Mulyadi , Gholib Gholib , Hafizuddin Hafizuddin , Zamzami Rumi Sahara , Bahi Muhammad
Published: June 2020

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.
Goblet Cells Proliferation of Duodenum, Jejunum, and Ileum of Laying Hens Immunized with Protein of Excretory-Secretory of Ascaridia galli
Authors: Balqis Ummu , Tiuria Risa , Priosoeryanto Bambang Pontjo , D Darmawi
Published: June 2020

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost’s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
The Characterization of Haemaglutinin Protein of Vili Vibrio alginolyticus as Adhesion Molecule on Epithelial Cells Receptor of Humpback Grouper (Cromileptes altivelis)
Authors: Yanuhar Uun
Published: June 2020

Vibriosis was a crucial problem and a primer infection causing mortality of humpback grouper.Virulence factor of vibrio, such as vili, is an adhesion molecule protein based on haemaglutination test toblood serum of humpback grouper. This research was aimed to characterize an adhesion molecule of viliprotein of V. alginolyticus on epithelial cells of Humpback grouper as haemaglutinin protein. Themethods are laboratory exploration by isolate epithelial cells of Humpback grouper, characterize a receptorand test vili protein to adhesion molecule that is epithelial cell of Humpback grouper. The result of proteinhaemaglutinin test to vili V. alginolyticus show that an adhesion molecule with a molecule weight of38.98 kDa is an adhesin vili V. alginolyticus and a haemaglutinin. The adhesion pattern of V.alginolyticus on epithelial cells of Humpback grouper exposed to protein haemaglutinin of vili show bothdiffuse and localize pattern. Research conclusion was the haemaglutinin protein of vili V. alginolyticuswas an adhesin having a role to both virulence process of bacteria and attachment process to receptor ofepithelial cells of Humpback grouper.Keywords: adhesion molecule, epithelial cell, vili, V. alginolyticus, C. altivelis
Decrease of Spermatogonia, Spermatocytes and Sertoli Cell Counts Male Mice (Mus musculus) Induced by Ethylene Glycol
Authors: Latief Nuraini
Published: June 2020

The experiment was conducted to study the effect of ethylene glycol (EG) on disturbance of spermatogenesis process of the male mice. Sixteen mice at the age of 2 months were randomly into 4 groups of 4 each. The mice of control group were only given drinking water without EG. Group KPI, KP2, and KP3 were given EG 5, 10, and 15 ml/liter drinking water, respectively. On day 31 of post treatment, all mice were killed for their testis. The testis were collected and processed for microscopic examination and haematoxylin-eosin staining. The result showed that EG given orally for 30 days on male mice have  negative effect on the process of spermatogenesis as the numbers of spermatogonia, spermatocyte and Sertoli cells decreased significantly (P0.01)
The Use of Multiple Linear Regression Model for Predicting The Influence of Weather to The Prevalence of Newcastle Disease in Chicken in North Aceh
Authors: Abrar Mahdi
Published: June 2020

The objective of this research is to see the influence of weather on the prevalence of Newcastle Disease (ND) in chicken in Kabupaten Aceh Utara (North Aceh). Data used in this research were obtained from Dinas Peternakan North Aceh for the number of chicken suffered ND and from Badan Meteorologi dan Geofisika Lhokseumawe, North Aceh for the form of weather. Multiple Linear Regression Model with five independent variables (the average of rainfall per month, the average of maximum temperature, the average of minimum temperature, the velocity of the wind, and the average of humidity per month) was used to see the influence of wheather to the prevalence of Newcastle Disease. Proportion the number of chicken suffered from ND which is the ratio of the number of chicken suffered from ND to the total number of chicken was used as dependent variables. The result shows that the best model is Ŷ= 120.529278 – 1.33 x wind humidity + 1.907 x wind velocity.
PENGARUH LAMA MATURASI DAN LAMA INKUBASI FERTILISASI TERHADAP ANGKA FERTILITAS OOSIT SAPI PERANAKAN ONGOLE SECARA IN VITRO
Authors: Kusindarta Dwi Liliek
Published: June 2020

Penelitian ini dilakukan untuk mengetahui pengaruh lama maturasi dan lama inkubasi fertilisasi terhadap angka fertilitas oosit sapi peranakan Ongole (PO) secara in vitro. Oosit diaspirasi dari folikel ovarium yang berdiameter 2-6 mm. Oosit dirandom untuk penelitian menggunakan rancangan blok acak sempurna (RCBD), dengan lama maturasi 20, 22 dan 24 jam sebagai blok dan lama inkubasi fertilisasi 4, 5 dan 6 jam sebagai perlakuan. Hasil penelitian menunjukkan angka fertilitas oosit sapi PO dengan lama maturasi 20 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masing-masing adalah 48,86%+3,88, 48,08%+2,22 dan 51,96%+ 2,27. Angka fertilitas oosit sapi PO dengan lama maturasi 22 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masing-masing adalah 50,14%+4,18, 51,14%+4,35 dan 52,27%+2,26. Angka fertilitas oosit sapi PO dengan lama maturasi 24 jam dan lama inkubasi fertilisasi 4, 5 dan 6 jam masingmasing adalah 51,21%+4,49, 53,49%+2,43 dan 50,96%+4,18. Tidak ada perbedaan yang nyata (P0,05) angka fertilitas antara lama maturasi 20, 22 dan 24 jam dan tidak ada perbedaan yang nyata (P0,05)  angka fertilitas antara lama inkubasi fertilisasi 4, 5 dan 6 jam.
KONSENTRASI PROTEIN DAN PENENTUAN BERAT MOLEKUL EKSKRETORI/SEKRETORI L3 Ascaridia galli
Authors: D Darmawi , Balqis Ummu , Tiuria Risa , Soejoedono Retno D , Pasaribu Fachriyan H
Published: June 2020

Penelitian ini bertujuan menentukan konsentrasi dan berat molekul protein  ekskretori/sekretori larva (L3) Ascaridia galli (A. galli). Larva L3 diperoleh dari usus halus 100  ayam tujuh hari setelah pemberian dosis 6000 L2 melalui esofagus ayam. Sebanyak 5–10  L3 dikultur secara in vitro  dalam setiap ml medium Rosswell Park Memorial Institute (RPMI 1640), pH 6,8, tanpa merah fenol dalam inkubator pada temperatur 37 0C dan 5% CO2 selama 3 hari. Ke dalam medium ditambahkan 100 unit ml-1 penisilin G, 100 µg ml-1 streptomisin, 5 µg ml-1 gentamisin dan 0,25 µg ml-1 kanamisin. Ekskretori/sekretori dipreparasi dari produk metabolisme L3 yang dilepaskan ke dalam medium kultur. Untuk mendapatkan protein ekskretori/sekretori, medium kultur dipekatkan dengan vivaspin 30.000 MWCO, dan kuantitas protein dihitung dengan metode Bradford. Berat molekul protein ekskretori/sekretori divisualisasikan dengan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Hasil penelitian  menunjukkan bahwa konsentrasi protein ekskretori/sekretori adalah 0,595 mg/ml dengan berat molekul 28 kDa.
Pathogenicity of Eimeria mivati Inoculation in Broiler Chicken
Authors: Hasan M
Published: June 2020

The propagation of E. mivati local strain was used single cell inoculation.  The objective of this study was to determine the pathogenicity of Eimeria mivati inoculation in broiler chicken by measuring the growth rate, oocysts production and levels of changes in small intestine.  The chickens were devided into two age groups of infection, three and teen days old.  Each group was received 1x104, 1x105   and 1x106  oocysts/chick.  The chichen were raised in wire cages and sampled accordingly.  Data collected was analyzed using analysis of variance. A large number of oocysts production, intestinal villi lesions and depression of growth rate were seen after infection.  Most of the peak production of oocysts were accured after the 9th day of infection.  However, after the peak production of oocysts,  oocysts production decreased extremely.  The choice of parasitic development were in the surface epithelial cells of the villi and the region dominantly effected was the third anterior of small intestine. The result revealed no significant differences regarding photogenicity findings (P0.05) between chicken aged three and ten days after infected with E. mivati.
The Development of Ascaridia galli Infective Eggs by In Vitro Culture
Authors: Balqis Ummu , D Darmawi , Hambal Muhammad , Tiuria Risa
Published: June 2020

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs
LABORATORY ASSESSMENT OF HYDRATION STATUS OF PRE-RUMINANT ETAWAH GOATS WITH DIARRHEA (Penentuan Status Hidrasi secara Laboratoris pada Anak Kambing Peranakan Etawah yang Mengalami Diare)
Authors: Roslizawaty R
Published: June 2020

Penelitian ini bertujuan mengembangkan panduan yang objektif dan akurat dalam memperhitungkan keadaan hidrasi anak kambing peranakan etawah yang masih menyusui yang mengalami diare. Penelitian inimenggunakan sepuluh ekor anak kambing peranakan etawah yang masih menyusu umur 10-14 hari, jenis kelamin jantan. Dehidrasi diinduksi dengan pemberian preparat diuretika (furosemide 2 mg/kg berat badan secara intramuskulus setiap 4 jam, spironolactone 50 mg per oral setiap 8 jam dan hydrochlorothiazide 50 mg per oral setiap 8 jam) dan larutan katartika (larutan sukrosa 2 mg/kg berat badan dalam 20% larutan aqua, per oral setiap 8 jam) selama 24 jam. Analisis regresi linier digunakan untuk menguji hubungan antara faktor-faktor laboratorisyang dianggap potensial untuk mengevaluasi status hidrasi (packed cell volume, hemoglobin dan total protein plasma dan dehidrasi). Penentuan derajat dehidrasi ditentukan berdasarkan persentase penurunan berat badan. Hasil penelitian menunjukkan bahwa total protein plasma (R2=0,95) berhubungan erat dengan status hidrasi dandapat digunakan sebagai prediktor untuk mengevaluasi status hidrasi pada anak kambing yang masih menyusu.Kata kunci: dehidrasi, anak kambing, derajat dehidrasi
Production of IgY Specific Antibody Against Entero Pathogenic Escherichia coli (EPEC) in Egg Yolk
Authors: Teguh Wibawan I Wayan , Pasaribu Fachriyan H. , Rawendra Rudi
Published: June 2020

Antibody to Enteropathogenic Eschericia coli (EPEC) K1.1. can be produced in egg yolk by the application of inactivated bacterial cells intravenously in layer chicken. The presence of specific antibody in sera and egg can bedetected with immunodiffusion techniques (Agar Gel Precipitation Test/AGPT), expressed by the occurrence of specific precipitation reaction between antibody and homolog antigen and no cross reaction of this antibody with antigen of Salmonella sp. and Klebsiella sp. The presence of specific antibody previously can be detected in sera, then 1 week after this the antibody start to be able be detected in egg yolk. The antibody is still present in sera as well as in egg yolk in large amount for 7 weeks and decrease significantly in the 8th week. This results indicated that theegg can be used in the producing specific antibody in the large quantity.
PENGARUH EKSTRAK ETANOL DAUN JALOH (Salix Tetrasperma Roxb) TERHADAP PERSENTASE PARASITEMIA PADA MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium berghei
Authors: Asmilia Nuzul , Sugito Sugito , Rahmi Erdiansyah , Febrianto Niko
Published: June 2020

This study aimed to verify the effect of ethanol extract of jaloh leaves at various doses on parasitemia percentage of in mice (Mus musculus), wich inoculated Plasmodium berghei. This study used 12 male mice (Mus musculus) strains Balb / C with average body weight of 30.2 grams of 2-month-old. This research using Completely Randomized Design (CRD) with four treatments and three replications. All the mice were infected with Plasmodium berghei prior to treatment on three days post infected the mice in group 2,3 and 4 were given with extract ethanol jaloh leaves at dose 50, 100, 150 mg/kgbw inspectively. The mice in group 1 (K0) were not given anything and consident as control.Observations parasitemia percentage using microscopy on preparations stained thin blood smear Giemsa. The parasitemia percentage data were analyzed using one way ANOVA. On the average (±SD) percentage of parasitemia during the three days in four treatments (K0, K1, K2, and K3) on the first day were 24.87±1.86; 24.33±1.53; 24.20±1.06; and 24.93±1.01. On the second day were 22.67±2.52; 23.00±1.00; 22.80±1.20; and 24.13±2.01; and on the third day were 24.77±1.66; 22.97±3.76; 21.13±1.90; and 24.27±1.42. Statistical analysis showed that ethanol extract of the jaloh leaves for KO, KI, KII, and KIV respectively treatment did not affect the percentage of parasitemia in mice (Mus musculus) inoculated Plasmodium berghei.
Microscopical Study of Pancreatic Tissue of Babirusa (Babyrousa babyrussa) Using Conventional and Immunohistochemical Methods
Authors: Adnyane Ketut Mudite , Macdonald Alastair A , Agungpriyono Srihadi
Published: June 2020

This study utilized conventional and immunohistochemical methods, to describe the morphology and distribution of glucagon and insulin cells in the pancreas of adult male babirusa (Babyrousa babyrussa). The results of research showed that the endocrine portion (Langerhans islet) spread evenly on the acinar portion of pancreas. Observation using immunohistochemical method showed that glucagon cells were distributed on the perifer, whereas insulin cells in the center of the Langerhans islet of pancreas. In general, the number of insulin cells was higher than glucagon cells. It is interesting to note, in the pancreas babirusa also found that neonatal elements similar to the Langerhans islet, but have larger sizes (ranging between 200 -700 mm). The picture is similar to the large Langerhans islet was reported in ruminant.  These cells give a negative reaction to insulin and glucagon antisera.
Invasif dan Persisten Pasteurella multocida Setelah Diinokulasi Secara Intratracheal pada Kalkun
Authors: Abrar Mahdi , Fakhrurrazi Fakhrurrazi
Published: June 2020

The objective of this study was to evaluate the invasiveness and persistence of Pasteurella multocida after intratracheal inoculation on turkeys. Four strains of Pasteurella multocida, P-1059, T-325, CU, and M-9 were used in this study and intratracheally (IT) inoculated into turkeys. The number of viable bacteria in the trachea, lung, liver, spleen, and the blood were enumerated during periods of 6 and 9 hrs post inoculation (PI). The result showed that four strains of organism were present in the organ and the tissues observed. In the liver, spleen, and blood, the virulent P-1059 strain showed significant increases within 6 or 9 hrs PI. Strain CU showed significant increase only in the spleen. Two other strains did not show significant increase in any organs; strain M-9, on the other hand, showed significant decrease in the trachea. These results indicated that P-1059 and three other strains showed their ability to invade the tissues or organs when they are intratracheally inoculated.
Polyoxyethylene Quaternary Ammonium Gemini Surfactants as a Completion Fluid Additive to Mitigate Formation Damage
Authors: Tariq Zeeshan , Kamal Muhammad Shahzad , Mahmoud Mohamed , Hussain Syed Muhammad , Abdulraheem Abdulazeez , Zhou Xianmin
Published: June 2020

During well completion operations, the wells are killed with specific fluids to control the well. These fluids can invade and damage the formation because of fluid/rock interactions. Fluids such as fresh water, brines, and weighted fluids (e.g., barite weighted, calcite weighted, and bentonite weighted) are used to control the formations during completion operations. These fluids can invade and interact with clays and damage the formation. In addition, these fluids may alter the near-wellbore wettability and make them more oil-wet, thereby affecting the production from these formations. In this work, polyoxyethylene quaternary ammonium gemini surfactants with different types of spacers are proposed as clay swelling additives in completion fluids to mitigate the formation damage in unconventional reservoirs. Adding the new surfactants will maintain the in-situ permeability and avoid the formation damage. The novel gemini surfactants are tested on unconventional tight sandstone formation enriched with high clay content to mitigate the formation damage during well completion. The process involved a complete stabilization of clays using gemini surfactants added in deionized water (DW). Coreflooding experiments were carried out on Scioto sandstone rock samples with an average porosity of 15.6% and average absolute permeability of 0.25 md. Several coreflooding experiments were carried out with different fluids, such as potassium chloride (KCl), sodium chloride (NaCl), and different classes of gemini surfactants. Coreflooding experiments were designed in a way that the cores were preflushed with the subjected fluid and then post-flooded with DW. Results showed that the cores saturated with KCl and NaCl solutions lost permeability significantly when flooded with water while gemini surfactant solutions maintained the same permeability even after being treated with DW. Conditioning with the KCl solution resulted in a 38% reduction of permeability and that with NaCl solution resulted in an 80% reduction of permeability when treated with DW. No significant change of permeability was found for the case of gemini surfactants. This indicates that the synthesized surfactants can be used for well completion operation without any side effects.