bluuebunny/crossref_metadata_2025 huggingface dataset:
Fatigue life prediction of lattice structures using a strain-life method based on an equivalent solid model
Authors: Gorguluarslan Recep M , Ozay Arda I
Published: January 2023

In this study, an efficient fatigue life estimation approach is proposed based on an equivalent solid model, along with two stress concentration factors, instead of a lattice model to simplify simulations for the strain-life method. One of the stress concentration factors accounts for the stress concentrations at the strut joints in lattice structures without any manufacturing effect, while the other accounts for the stress concentrations due to surface defects on the struts introduced by the additive manufacturing process. A simple solid cell, for which the dimensions are the same as those of the lattice cell, is first used to estimate the fatigue life of the bulk material used in the additive manufacturing process using the Brown–Miller strain-life method. Combinations of two stress concentration factors were then utilized to predict the fatigue life of the lattice structure from the equivalent solid model. Using this approach, computationally expensive lattice structure simulations were eliminated, and the fatigue life of the lattice structure was predicted directly from the bulk material fatigue life estimation. The proposed approach was applied to different lattice types made of Ti-6Al-4V alloy using the laser-powder bed fusion additive manufacturing process. The fatigue life results estimated using the proposed approach were in good agreement with the experimental fatigue life results reported in the literature.
QEEG Characterizations During Hyperventilation, Writing and Reading Conditions: A Pre–Post Cognitive-Behavioral Intervention Study on Students with Learning Difficulty
Authors: Kaushik Pratima
Published: January 2023

Background: Electroencephalography (EEG) has been used to measure neural correlates of cognitive and social development in children for decades. It is essential to evaluate the relationship between EEG parameters and cognitive measures to understand the mechanisms of learning problems better. Methods and procedure: Fifty school-going children with complaints of learning problems were studied. EEG and other cognitive measures were used to assess children before and after PEABLS; a cognitive-behavioral intervention was imparted. EEG was recorded while hyperventilation, writing, and reading conditions, and the values for absolute and relative powers were calculated. Results: The results suggested that the post-intervention absolute (in the theta and alpha bands) and relative (delta, theta, and alpha) power values were higher, and the relative power beta value was significantly lower at most of the electrodes in comparison to pre-intervention EEG measures. A significant high positive correlation in the children with learning problems between the relative power of alpha, beta O1O2, the relative power of theta, delta T3T4, and the academic scores, IQ, working memory, DTLD, and BGT values. Conclusion: These quantitative electroencephalogram findings in children with learning problems are related to cognitive measures. The findings could be due to brain immaturity and lack of learning opportunities.
Antonio Scarpa (1752-1832): His Contribution to the History of Surgery
Authors: Ferretti Cecilia , Stroppa Daniele , Riva Michele Augusto
Published: January 2023

Background

Antonio Scarpa (1752-1832) is generally remembered for his discoveries in the field of anatomy, but he also provided some important contributions to the history of surgery.

Methods

We analyzed main treatises written by Scarpa, highlighting how he contributed to the development of modern surgery.

Results

Scarpa made great innovations in three branches of surgery: abdominal surgery (hernia), orthopedics (clubfoot) and vascular surgery (aneurism). Furthermore, he contributed to the birth of modern ophthalmology.
Structural Influences on Consent Decisions in Participatory Health Research in Eswatini
Authors: Brear Michelle R. , Shabangu Pinky N. , Hammarberg Karin , Fisher Jane
Published: January 2023

Recognition that structural factors influence participation decisions and have potential to coerce participation, emerged relatively recently in research ethics literature. Empirical evidence to elucidate the nature of “structural” coercion and influence is needed to optimise respect for autonomy through voluntary informed consent. We present findings from ethnographic data about community co-researchers’ experiences designing and implementing demographic and health survey consent procedures in participatory health research in Eswatini. Informed by Bourdieu's sociological theory of multiple types of capital/power, our findings detail structural influences on research participation decisions, highlight the inherently power-laden dynamics of consent interactions, and suggest that to be optimally ethical, research ethics principles and practices should consider and account for structural power dynamics.
Paediatric interlabial bleeding: More than vaginal pathology
Authors: Wong Zeng Hao Joel
Published: January 2023

A 10 years old girl was referred with the complaints of “per vaginal bleeding and a mass after cycling”. During the first 2 weeks of symptoms, she had visited three different doctors with possible delayed recognition of her diagnosis. Clinical examination revealed an underweight, non-dysmorphic, prepubertal girl with vulvitis and an inflamed interlabial bleeding mass with ulceration. Imaging revealed a subcentimetre arteriovenous fistula and haematoma in the vagina. After one more week (total 3 weeks) of medical therapy, the indication for surgery was worsening of symptoms and failure of medical management. Examination under anaesthesia and cystoscopy revealed a complete urethra prolapse. Dilute adrenaline was injected into the prolapsed mucosa. The prolapse was excised in elliptical segments, and the edges closed with interrupted absorbable sutures. Sub-centimetre segments of mucosa were left between the excised specimens. Bipolar diathermy was used only for hemostasis and circumferential coagulation on the mucosa was avoided. These measures prevent an iatrogenic stenosis. The urethra prolapse was reduced, with no recurrence demonstrated on Valsalva maneuver. Post-operatively, her symptoms resolved. Oral antibiotics, sitz baths, antimicrobial cream and topical estrogen were administered. There was no recurrent urethra prolapse or stricture. The aim of this case report is to increase awareness of the urethral prolapse in the paediatric population and its management.
A sexuality pivot: Thinking through solidarity, geographies of sexuality, and a world in transition
Authors: Tucker Andrew
Published: January 2023

While work on solidarities forms a core element within geographical scholarship, especially as related to forms of shared connection to address forms of inequality, few studies from the geographies of sexualities literature have directly engaged with the concept. This article argues that there are two complementary logics as to why scholars working on sexuality may want to consider more forcibly the concept of solidarity. One of these logics suggests that a focus on solidarities can further pre-existing key interests within the geographies of sexualities literature, especially in parts of the world that have historically received relatively limited research attention. A second logic suggests that a focus on sexuality-based solidarities can help enable sexualities scholars to further critical engagement regarding broader discourses, and offer alternative framings, regarding a world in transition. This article presents a series of examples, drawn from across sub-Saharan Africa, related to both logics together with a series of potential future directions for research. Taken collectively, this article, by drawing on earlier work on solidarity from within the discipline of geography, contends that there are a number of key benefits for sexualities scholars to undertake a ‘pivot’ or choice to explore solidarity more directly in the current moment.
Bacteriology of community-acquired pneumonia, antimicrobial susceptibility pattern and associated risk factors among HIV patients, Northeast Ethiopia: cross-sectional study
Authors: Tilahun Mihret , Gebretsadik Daniel , Seid Abdurahaman , Gedefie Alemu , Belete Melaku Ashagrie , Tesfaye Melkam , Kebede Edosa , Shibabaw Agumas
Published: January 2023

Objective:

Pneumonia is an opportunistic infection and it is a major cause of mortality and morbidity among human immunodeficiency virus/acquired immune deficiency syndrome-positive patients. Previous studies have shown the dominant pathogens bacterial isolates were K. pneumoniae 27.0%, S. aureus 20.8%, S. pneumoniae 18.8% and E. coli 8.3%. This study aimed to determine bacteriology of community-acquired pneumonia, antimicrobial susceptibility pattern and associated risk factors among human immunodeficiency virus patients in the Northeast Ethiopia: cross-sectional study.

Methods:

A health facility-based cross-sectional study was conducted from January to April 2021 at six health facilities in Dessie Town. A total of 378 community-acquired pneumonia patients suspected to be human immunodeficiency virus-positive were recruited using a consecutive sampling technique. Sociodemographic and clinical data were collected using a structured questionnaire. A two-milliliter sputum specimen was collected aseptically from each study participant. Samples were cultivated on blood agar, chocolate agar and MacConkey agar to isolate bacterial pathogens. To identify bacteria pathogens Gram stain, colony morphology and biochemical tests were performed. The Kirby-Bauer Disc Diffusion method was used to perform the antimicrobial susceptibility test. Descriptive statistics, logistic regression analysis was carried out using Statistical package for social science version 25 software. p-value < 0.05 with a corresponding 95% confidence interval (CI) was considered for statistical significance.

Result:

The overall prevalence of bacterial pneumonia was 175 (46.3%). Gram-negative bacteria accounted for 119 (68%) and the predominant isolates identified were Streptococcus pneumoniae 49 (28%) followed by Klebsiella pneumoniae 46 (26.3%), Pseudomonas aeruginosa 34 (19.4%). There were 148 (84.6%) multidrug-resistant bacteria overall. Statistically significant factors included viral load, cigarette smoking, cluster of differentiation 4 count, alcohol use, World Health Organization clinical stages III and IV and low white blood cell count.

Conclusion:

The study found that both multidrug resistance and bacterial pneumonia were high. Thus, bacterial culture and antimicrobial susceptibility tests should be routinely performed in health facilities in order to prevent and control the spread of bacterial infection and concurrent drug resistance.
School Counselor Professional Identity in Relation to Post-Master’s Supervision
Authors: Mecadon-Mann Melissa , Tuttle Malti
Published: January 2023

School counselors may choose to pursue clinical supervision after they graduate from a master’s program even though they are not required to do so. This quantitative study utilized a survey research design to examine the differences between school counselor professional identity (SCPI) development and post-master’s supervision. Results from this study indicate significant differences in components of SCPI between school counselors who participated in post-master’s supervision and those who did not. School counselors who pursued post-master’s supervision had stronger identity scores focused on roles and responsibilities, leadership, and social justice advocacy.
The Politics of Belonging and Implications for School Organization: Autophotographic Perspectives on “Fitting In” at School
Authors: Walls Jeff , Louis Karen Seashore
Published: January 2023

The notion of belonging is an often-referenced but under-theorized concept in studies of school organization. The purpose of this study is to examine the politics of belonging in schools and accompanying implications for how schools are organized and led. This research employs an autophotographic methodology. Student participants took photographs across 2 years of data collection of spaces where they did and did not “fit in” and participated in interviews to explain their photographs. Students identified four themes in their photographs regarding their sense of membership at school: (a) the importance of spaces where belonging is noncontingent; (b) the distinction between calm spaces and surveilled spaces; (c) anxiety in public, “wild” spaces where no help was available; and (d) generally positive but mixed impressions of teachers. An increased understanding of organization leadership for belonging is linked to numerous other timely concerns in educational administration, including equity and inclusion.
Patterns of multimorbidity among a community-based cohort in rural India
Authors: Gummidi Balaji , Gautam Vaishali , John Oommen , Ghosh Arpita , Jha Vivekanand
Published: January 2023

Background

Multimorbidity estimates are expected to increase in India primarily due to the population aging. However, there is a lack of research estimating the burden of multimorbidity in the Indian context using a validated tool. We estimated the prevalence and determinants of multimorbidity amongst the adult population of the rural Uddanam region, Andhra Pradesh.

Methods

This community-based cross-sectional study was conducted as a part of an ongoing research program. Multistage cluster sampling technique was used to select 2419 adult participants from 40 clusters. Multimorbidity was assessed using Multimorbidity Assessment Questionnaire for Primary Care (MAQ-PC) tool, collecting information on 13 chronic diseases. Patient Health Questionnaire (PHQ-12) was used to screen for depression. Multiple logistic regression was conducted to identify the strongest determinants of multimorbidity.

Results

Of the 2419 participants, 2289 completed the MAQ-PC tool. Mean age (standard deviation) of participants was 48.1 (13.1) years. The overall prevalence of multimorbidity was 58.5% (95% CI 56.5-60.6); with 30.7%, 15.6%, and 12.2% reporting two, three, and four chronic conditions, respectively. Acid peptic disease-musculoskeletal disease (44%) and acid peptic disease-musculoskeletal disease-hypertension (14.9%) were the most common dyad and triad. Among metabolic diseases, diabetes-hypertension (28.3%) and diabetes-hypertension-chronic kidney disease (7.6%) were the most common dyad and triad, respectively. Advancing age, female gender, and being obese were the strongest determinates of the presence of multimorbidity. Depression was highly prevalent among the study population, and participants with higher PHQ-12 score had 3.7 (2.5-5.4) greater odds of having multimorbidity.

Conclusions

Our findings suggest that six of 10 adults in rural India are affected with multimorbidity. We report a higher prevalence of multimorbidity as compared with other studies conducted in India. We also identified vulnerable groups which would guide policy makers in developing holistic care packages for individuals with multimorbidity.
Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus
Authors: Cai Yidan , Wei Ke
Published: January 2023

Polyploidy and the microbiome are crucial factors in how a host organism responds to disease. However, little is known about how triploidization and microbiome affect the immune response and disease resistance in the fish host. Therefore, this study aims to identify the relationship between intestinal microbiota composition, transcriptome changes, and disease resistance in triploid Carassius auratus (3nCC). In China’s central Dongting lake water system, diploid (2nCC) and triploid Carassius auratus were collected, then 16S rRNA and mRNA sequencing were used to examine the microbes and gene expression in the intestines. 16S rRNA sequencing demonstrated that triploidization altered intestinal richness, as well as the diversity of commensal bacteria in 3nCC. In addition, the abundance of the genus Vibrio in 3nCC was increased compared to 2nCC (P < 0.05). Furthermore, differential expression analysis of 3nCC revealed profound up-regulation of 293 transcripts, while 324 were down-regulated. Several differentially expressed transcripts were related to the immune response pathway in 3nCC, including NLRP3, LY9, PNMA1, MR1, PELI1, NOTCH2, NFIL3, and NLRC4. Taken together, triploidization can alter bacteria composition and abundance, which can in turn result in changes in expression of genes. This study offers an opportunity for deciphering the molecular mechanism underlying disease resistance after triploidization.
Where, why and who delivers our babies? Examining the perspectives of women on utilization of antenatal and delivery services in a developing country
Authors: Ossai Edmund Ndudi , Eze Irene Ifeyinwa , Eke Pearl Chizobam , Onah Cosmas Kenan , Agu Chibuike , Ogbonnaya Lawrence Ulu
Published: January 2023

Background

The differences in maternal mortality between developed and developing countries is due to differences in use of antenatal and delivery services. The study was designed to determine the views of women on utilization of antenatal and delivery services in urban and rural communities of Ebonyi state, Nigeria.

Methods

Community based descriptive exploratory study design was employed. Qualitative data was collected through use of pre-tested focus group discussion (FGD) guide. Eight FGDs were conducted among women who were pregnant and others who have delivered babies one year prior to the study. Four FGDs each were conducted in urban and rural communities. QDA Miner Lite v2.0.6 was used in the analysis of the data.

Results

Most of the participants in urban and rural areas prefer the man and woman deciding on where to receive antenatal and deliver care. All the participants in urban and rural communities wish for the support of their husbands when pregnant. Perceived quality of care is the major reason the women choose a facility for antenatal and delivery services. Others reasons included cost of services and proximity to a facility. Participants in rural communities were of the opinion that traditional birth attendants deliver unique services including helping women to achieve conception. For participants in urban, traditional birth attendants are very friendly and perhaps on divine assignment. These reasons explain why women still patronize their services. The major criticism of services of traditional birth attendants is their inability to manage complications associated with pregnancy and delivery. The major reasons why women delivery at home included poverty and cultural beliefs.

Conclusions

All efforts should be made to reduce the huge maternal death burden in Nigeria. This may necessitate the involvement of men and by extension communities in antenatal and delivery matters. There is need to train health workers in orthodox health facilities on delivery of quality healthcare. Public enlightenment on importance of health facility delivery will be of essence. Encouraging women to deliver in health facilities should be prioritized. This may entail the provision of free or subsidized delivery services. The deficiencies of primary health centers especially in rural communities should be addressed.
Vibration therapy in young children with mild to moderate cerebral palsy: does frequency and treatment duration matter? A randomised-controlled study
Authors: Adaikina Alena , Derraik José G. B. , Hofman Paul L. , Gusso Silmara
Published: January 2023

Background

Vibration therapy (VT) has been increasingly studied in children with cerebral palsy (CP) over the last years, however, optimal therapeutic VT protocols are yet to be determined. The present study compared the effects of side-alternating VT protocols varying in frequency and treatment duration on the health of young children with mild-to-moderate CP.

Methods

Thirty-four participants aged 6.0 to 12.6 years with CP acted as their own controls and underwent two consecutive study periods: a 12-week lead-in (control) period prior to the intervention period of 20-week side-alternating VT (9 min/session, 4 days/week), with the frequency either 20 Hz or 25 Hz, determined by randomisation. Participants had 4 assessment visits: baseline, after the control period, after 12-week VT (12VT), and after further 8 weeks of VT (20VT). Assessments included 6-minute walk test (6MWT); dual-energy x-ray absorptiometry; gross motor function; muscle function testing on the Leonardo mechanography plate and by hand-held dynamometry, and a quality-of-life questionnaire (CP QOL). Analysis was carried out using linear mixed models based on repeated measures.

Results

Side-alternating VT was well-tolerated, with occasional mild itchiness reported. The median compliance level was 99%. VT led to improvements in 6MWT (+ 23 m; p = 0.007 after 20VT), gross motor function in standing skills (+ 0.8 points; p = 0.008 after 12VT; and + 1.3 points; p = 0.001 after 20VT) and in walking, running and jumping skills (+ 2.5 points; p < 0.0001 after 12VT; and + 3.7 points; p < 0.0001 after 20VT), spine bone mineral density z-score (+ 0.14; p = 0.015 after 20VT), velocity rise maximum of the chair rising test (+ 0.14 m/s; p = 0.021 after 20VT), force maximum of the single two-leg jump test (+ 0.30 N/kg; p = 0.0005 after 12VT; and + 0.46 N/kg; p = 0.022 after 20VT) and in the health module of CP QOL (+ 7 points; p = 0.0095 after 20VT). There were no observed differences between the two VT frequencies (i.e., 20 Hz vs 25 Hz) on study outcomes.

Conclusions

The study confirms that side-alternating VT has positive effects on mobility, gross motor function, body composition, muscle function, and quality of life, independent of VT frequencies tested. Long-term, 20VT appears to be a more efficient treatment duration than a short-term, 12VT.

Trial registration

Australian New Zealand Clinical Trials Registry ACTRN12618002026202; 18/12/2018.
Analysis of factors influencing the use of child restraint system by parents of children aged 0–6 years: an information, motivation, behavioral skills model-based cross-sectional study
Authors: Sun Yaru , Liu Ting , Chen Junyu , Huang Juan , Wang Xin , Lu Mingqin , Luo Ying , Yang Xiuling
Published: January 2023

Background

Children's injuries from traffic accidents have been identified as a global public health issue. Child restraint system (CRS) is a useful tool for lowering the risk of injury to children. Nevertheless, CRS usage is really low in China. The goal of the current study was to investigate the use of CRS after the legislation revised in China and to explore the influencing factors based on Information, Motivation, and Behavioral Skills model (IMB).

Methods

The study is a cross-sectional survey of parents who took their 0 to 6-year-old children for seeking primary care services at the Children Preventive Health Care Clinic of a tertiary hospital in Shandong Province, China. Parents were invited to complete the self-administered questionnaire between March and June 2022, including their knowledge, motivation, and behavioral skills, use behavior of CRS and socio-demographics. Ordinal logistic regression was used to explore the factors associated with CRS use by using SPSS software (version 26.0).

Results

In total, 442 parents participated in the study; 56.1% (n = 201) of the parents utilized CRS for their child passengers, however only 29.0% used CRS frequently. The result of logistic regression analysis show that parents with junior college (OR = 0.398, 95%CI: 0.185 ~ 0.857), possessing a high family economic status(OR = 0.225, 95%CI: 0.088 ~ 0.578), being trained on children’s unintentional injuries(OR = 0.435,95%CI: 0.272 ~ 0.695), and having high scores on CRS riding mode cognition(OR = 0.476, 95%CI: 0.368 ~ 0.616), CRS type cognition(OR = 0.519, 95%CI: 0.392 ~ 0.689), CRS use motivation(OR = 0.392, 95%CI: 0.295 ~ 0.520) and installation skills(OR = 0.559, 95%CI:0.411 ~ 0.761) were the main factors promoting the usage of CRS.

Conclusions

This study found that the use of CRS can be increased by improving parents' knowledge, motivation and behavior skills and hence related educational programs is necessary for increasing CRS use in China.
Identification of key module and hub genes in pulpitis using weighted gene co-expression network analysis
Authors: Zhang Denghui , Zheng Chen , Zhu Tianer , Yang Fan , Zhou Yiqun
Published: January 2023

Background

Pulpitis is a common disease mainly caused by bacteria. Conventional approaches of diagnosing the state of dental pulp are mainly based on clinical symptoms, thereby harbor deficiencies. The accurate and rapid diagnosis of pulpitis is important for choosing the suitable therapy. The study aimed to identify pulpits related key genes by integrating micro-array data analysis and systems biology network-based methods such as weighted gene co-expression network analysis (WGCNA).

Methods

The micro-array data of 13 inflamed pulp and 11 normal pulp were acquired from Gene Expression Omnibus (GEO). WGCNA was utilized to establish a genetic network and categorize genes into diverse modules. Hub genes in the most associated module to pulpitis were screened out using high module group members (MM) methods. Pulpitis model in rat was constructed and iRoot BP plus was applied to cap pulp. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used for validation of hub genes.

Results

WGCNA was established and genes were categorized into 22 modules. The darkgrey module had the highest correlation with pulpitis among them. A total of 5 hub genes (HMOX1, LOX, ACTG1, STAT3, GNB5) were identified. RT-qPCR proved the differences in expression levels of HMOX1, LOX, ACTG1, STAT3, GNB5 in inflamed dental pulp. Pulp capping reversed the expression level of HMOX1, LOX, ACTG1.

Conclusion

The study was the first to produce a holistic view of pulpitis, screen out and validate hub genes involved in pulpitis using WGCNA method. Pulp capping using iRoot BP plus could reverse partial hub genes.
Knowledge of the ovulatory cycle and its determinants among women of childbearing age in Haiti: a population-based study using the 2016/2017 Haitian Demographic Health Survey
Authors: Jean Simon David , Jamali Yasmeen , Olorunsaiye Comfort Z. , Théodat Jean-Marie
Published: January 2023

Background

The knowledge of ovulatory cycle (KOC) is the base for natural family planning methods, yet few studies have paid attention to women’s KOC. This study aimed to assess the prevalence of correct KOC and its determinants among women of childbearing age in Haiti.

Methods

Data from the nationally representative cross-sectional Haiti Demographic and Health Survey 2016/17 were used. STATA/SE version 14 was employed to analyse the data by computing descriptive statistics, Chi‑square, and binary logistic regression model to assess the socio-economic and demographic predictors of correct KOC. P-value less than 0.05 was taken as a significant association.

Results

Out of 14,371 women of childbearing age who constituted our sample study, 24.1% (95% CI 23.4–24.8) had correct KOC. In addition, the findings showed that place of residence, respondent’s education level, wealth index, currently working, husband/partner’s education level, contraceptive use, exposure to mass media FP messages, and fieldworker visit were significantly associated with correct KOC.

Conclusion

Policies should include increasing the literacy at community level as well as of individual women and their partners. Moreover, increasing awareness about family planning should be prioritized, especially for women from poor households and rural areas.
Design of a new electrochemical aptasensor based on screen printed carbon electrode modified with gold nanoparticles for the detection of fumonisin B1 in maize flour
Authors: Naghshbandi Behnaz , Adabi Mohsen , Pooshang Bagheri Kamran , Tavakolipour Hamid
Published: January 2023

A new aptasensor for detecting fumonisin B1 (FB1) in the maize samples was developed based on DNA- aptamer recognition and electrochemical technique. A thiol-modified single-stranded DNA (ss-HSDNA) was immobilized on a screen printed carbon electrode (SPCE) electrodeposited by gold nanoparticles (AuNPs). The morphology and structure of SPCE and AuNPs/SPCE were evaluated via scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS). The SEM results demonstrated that the SPCE had a flat sheet-like structure, and the AuNPs were homogeneously electrodeposited on the SPCE. Cyclic voltammetry (CV) experiments in the [Fe(CN)6]− 3/− 4 solution were conducted to investigate each step of electrode modification as well as aptasensor performance. Aptamer-FB1 interaction prevented the electron transfer permitting the determination of FB1 in the range of 0.5–500 ng/mL with a low detection limit (0.14 ng/mL). The designed aptasensor was also shown high selectivity, acceptable repeatability and reproducibility, good long-term stability, and excellent recovery. Furthermore, there was a strong correlation between the findings achieved via the designed aptasensor and high performance liquid chromatography (HPLC). Therefore, a simple construction process and satisfactory electrochemical performance of the proposed aptasensor have a great potential for the detection of FB1 in maize samples.
Preference heterogeneity in health valuation: a latent class analysis of the Peru EQ-5D-5L values
Authors: Karim Suzana , Craig Benjamin Matthew , Tejada Romina Arely , Augustovski Federico
Published: January 2023

Background

Preference heterogeneity in health valuation has become a topic of greater discussion among health technology assessment agencies. To better understand heterogeneity within a national population, valuation studies may identify latent groups that place different absolute and relative importance (i.e., scale and taste parameters) on the attributes of health profiles.

Objective

Using discrete choice responses from a Peruvian valuation study, we estimated EQ-5D-5L values on a quality-adjusted life-year (QALY) scale accounting for latent heterogeneity in scale and taste, as well as controlling heteroskedasticity at task level variation.

Method

We conducted a series of latent class analyses, each including the 20 main effects of the EQ-5D-5L and a power function that relaxes the constant proportionality assumption (i.e., discounting) between value and lifespan. Taste class membership was conditional on respondent-specific characteristics and their experience with the composite time trade-off (cTTO) tasks. Scale class membership was conditional on behavioral characteristics such as survey duration and self-stated difficulty level in understanding tasks. Each analysis allowed the scale factor to vary by task type and completion time (i.e., heteroskedasticity).

Results

The results indicated three taste classes: a quality-of-life oriented class (33.35%) that placed the highest value on levels of severity, a length-of-life oriented class (26.72%) that placed the highest value on lifespan, and a middle class (39.71%) with health attribute effects lower than the quality class and lifespan effect lower than the length-of-life oriented class. The EQ-5D-5L values ranged from − 2.11 to 0.86 (quality-of-life oriented class), from − 0.38 to 1.02 (middle class), and from 0.36 to 1.01 (length-of-life oriented class). The likelihood of being a member of the quality-of-life class was highly dependent on whether the respondent completed the cTTO tasks (p-value < 0.001), which indicated that the cTTO tasks might cause the Peru respondents to inflate the burden of health problems on a QALY scale compared to those who did not complete the cTTO tasks. The results also showed two scale classes as well as heteroskedasticity within each scale class.

Conclusion

Accounting for taste and scale classes simultaneously improveds understanding of preference heterogeneity in health valuation. Future studies may confirm the differences in taste between classes in terms of the effect of quality of life and lifespan attributes. Furthermore, confirmatory evidence is needed on how behavioral variables captured within a study protocol may enhance analyses of preference heterogeneity.
Angiotensin-(1–7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways
Authors: Chen Xin-Sen , Cui Jing-Rui , Meng Xiang-Long , Wang Shu-Hang , Wei Wei , Gao Yu-Lei , Shou Song-Tao , Liu Yan-Cun , Chai Yan-Fen
Published: January 2023

Background

There is no available viable treatment for Sepsis-Induced Cardiomyopathy (SIC), a common sepsis complication with a higher fatality risk. The septic patients showed an abnormal activation of the renin angiotensin (Ang) aldosterone system (RAAS). However, it is not known how the Ang II and Ang-(1–7) affect SIC.

Methods

Peripheral plasma was collected from the Healthy Control (HC) and septic patients and Ang II and Ang-(1–7) protein concentrations were measured. The in vitro and in vivo models of SIC were developed using Lipopolysaccharide (LPS) to preliminarily explore the relationship between the SIC state, Ang II, and Ang-(1–7) levels, along with the protective function of exogenous Ang-(1–7) on SIC.

Results

Peripheral plasma Ang II and the Ang II/Ang-(1–7) levels in SIC-affected patients were elevated compared to the levels in HC and non-SIC patients, however, the HC showed higher Ang-(1–7) levels. Furthermore, peripheral plasma Ang II, Ang II/Ang-(1–7), and Ang-(1–7) levels in SIC patients were significantly correlated with the degree of myocardial injury. Additionally, exogenous Ang-(1–7) can attenuate inflammatory response, reduce oxidative stress, maintain mitochondrial dynamics homeostasis, and alleviate mitochondrial structural and functional damage by inhibiting nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thus alleviating SIC.

Conclusions

Plasma Ang-(1–7), Ang II, and Ang II/Ang-(1–7) levels were regarded as significant SIC biomarkers. In SIC, therapeutic targeting of RAAS, for example with Ang-(1–7), may exert protective roles against myocardial damage.
Hydrolysis of lignocellulose to succinic acid: a review of treatment methods and succinic acid applications
Authors: Zhou Shuzhen , Zhang Miaomiao , Zhu Linying , Zhao Xiaoling , Chen Junying , Chen Wei , Chang Chun
Published: January 2023

Succinic acid (SA) is an intermediate product of the tricarboxylic acid cycle (TCA) and is one of the most significant platform chemicals for the production of various derivatives with high added value. Due to the depletion of fossil raw materials and the demand for eco-friendly energy sources, SA biosynthesis from renewable energy sources is gaining attention for its environmental friendliness. This review comprehensively analyzes strategies for the bioconversion of lignocellulose to SA based on the lignocellulose pretreatment processes and cellulose hydrolysis and fermentation principles and highlights the research progress on acid production and SA utilization under different microbial culture conditions. In addition, the fermentation efficiency of different microbial strains for the production of SA and the main challenges were analyzed. The future application directions of SA derivatives were pointed out. It is expected that this research will provide a reference for the optimization of SA production from lignocellulose.
Metabolic and cardiovascular responses to continuous and intermittent plank exercises
Authors: Huang Zihao , Wang Biru , Song Kangping , Wu Shaoping , Kong Huimin , Guo Lan , Liang Qi
Published: January 2023

Background

Plank exercise (PE) is a whole-body isometric muscle training which is beneficial for physical health. However, none of the previous studies investigated the responses within a typical isometric muscle training or PE protocol consisting of multiple sets. The application of PE was restricted for the understudied metabolic and cardiovascular responses, especially for the patients with cardiovascular diseases. This study is to alleviate the safety concerns of PE by investigating the PE-induced metabolic and cardiovascular responses.

Methods

Eleven male recreational-level college students completed a baseline cardiopulmonary exercise test, continuous PE (CPE) and intermittent PE (IPE). Ratio of maximal oxygen uptake per kilogram of body mass (%VO2max/kg), ratio of maximal heart rate (%HRmax), and respiratory exchange ratio (RER) were continuously measured during PEs and divided into seven equal timepoints. Blood pressure (BP) was measured every minute during, before, and after PEs. A mixed-model repeated measures ANOVA was used to examine the interaction effect of exercise × phase.

Results

The %VO2max/kg (F6,69=11.25, P < 0.001), %HRmax (F6,65=7.74, P < 0.001), RER (F6,69=11.56, P < 0.001), and BP (systolic BP, F2,26=8.42, P = 0.002; diastolic BP, F2,24=22.63, P < 0.001) increased by safe magnitudes. Compared with the corresponding period in the IPE group, the %VO2max/kg (33.5 [2.2] vs. 27.7 [1.9], P = 0.043) and %HRmax (63.2 [3.9] vs. 53.3 [2.1], P = 0.019) increased more significantly from the 40% duration of CPE. Systolic BP increased by larger magnitudes during CPE than IPE (154.2 [3.8] vs. 142.3 [4.8] mmHg, P = 0.002). RERs were over 1 during PEs without cardiovascular and metabolic variables over the anaerobic threshold.

Conclusion

Energy was mainly supplied by anaerobic metabolism during PEs. CPE may be preferable for trainees aiming at anaerobic capacity enhancement. IPEs may be preferable to CPEs for youth patients with mild and borderline cardiovascular diseases due to their lower metabolic and cardiovascular responses.
Immunomodulatory effects of probiotic supplementation in patients with asthma: a randomized, double-blind, placebo-controlled trial
Authors: Sadrifar Sina , Abbasi-Dokht Tannaz , Forouzandeh Sarvenaz , Malek Farhad , Yousefi Bahman , Salek Farrokhi Amir , Karami Jafar , Baharlou Rasoul
Published: January 2023

Background

Asthma is considered to be a chronic inflammatory disorder of the airways. Probiotics are living microorganisms that are found in the human gut and have protective effects against a wide range of diseases such as allergies. The aim of this study was to investigate the improvement of clinical asthma symptoms and changes in the expression pattern of selective microRNAs in patients with asthma and the changes in IL-4 and IFN-γ plasma levels after receiving probiotics.

Materials and methods

The present study was a randomized, double-blind, placebo-controlled trial that enrolled 40 asthmatic patients. They were treated with probiotics or placebo: 1 capsule/day for 8 weeks. Pulmonary function tests, IL-4 and IFN-γ levels, and expression of microRNAs were assessed at baseline and after treatment.

Results

The results showed that the expression of miR-16, miR146-a and IL-4 levels in patients with asthma after receiving probiotic supplementation was significantly reduced and miR-133b expression was increased. In addition, pulmonary function tests showed a significant improvement in Forced Expiratory Volume in 1 s and Forced Vital Capacity after receiving probiotics.

Conclusion

In our study, 8-week treatment with probiotic supplementation led to reduced Th2 cells-associated IL-4 and improved Forced Expiratory Volume and Forced Vital Capacity. It appears probiotics can be used in addition to common asthma treatments.
Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption
Authors: He Zhengxin , Huo Xiaosai , Piao Jingzi
Published: January 2023

Nucleic acid based molecular technologies are the most promising tools for the early diagnosis of Candida infection. A simple and effective DNA preparation method is of critical for standardizing and applying molecular diagnostics in clinic laboratories. The goal of this study was to develop a Candida DNA preparation method that was quick to do, easy to perform, and bio-safe. Snailase and lyticase were screened and combined in this work to enhance the lysis of Candida cells. The lysis solution composition and metal bath were optimized to boost amplification efficiency and biosafety. A duplex real-time PCR was established to evaluate the sensitivity and specificity of the preparation method. Using the supernatant from the rapid preparation method as templates, the duplex PCR sensitivities for five common Candida species were determined to be as low as 100 CFUs. When compared to conventional preparation methods, the samples prepared by our method showed higher PCR detection sensitivity. PCR identification and ITS sequencing were 100% consistent, which was better than biochemical identification. This study demonstrates a rapid method for Candida DNA preparation that has the potential to be used in clinical laboratories. Meanwhile, the practical application of the method for clinical samples needs to be proven in future investigations.
Neural function of Bmal1: an overview
Authors: Zheng Yuanjia , Pan Lingyun , Wang Feixue , Yan Jinglan , Wang Taiyi , Xia Yucen , Yao Lin , Deng Kelin , Zheng Yuqi , Xia Xiaoye , Su Zhikai , Chen Hongjie , Lin Jie , Ding Zhenwei , Zhang Kaitong , Zhang Meng , Chen Yongjun
Published: January 2023

Bmal1 (Brain and muscle arnt-like, or Arntl) is a bHLH/PAS domain transcription factor central to the transcription/translation feedback loop of the biologic clock. Although Bmal1 is well-established as a major regulator of circadian rhythm, a growing number of studies in recent years have shown that dysfunction of Bmal1 underlies a variety of psychiatric, neurodegenerative-like, and endocrine metabolism-related disorders, as well as potential oncogenic roles. In this review, we systematically summarized Bmal1 expression in different brain regions, its neurological functions related or not to circadian rhythm and biological clock, and pathological phenotypes arising from Bmal1 knockout. This review also discusses oscillation and rhythmicity, especially in the suprachiasmatic nucleus, and provides perspective on future progress in Bmal1 research.
Inheritance of wood properties and their radial variations in full-sib families of 36-year-old Japanese larch (Larix kaempferi (Lamb.) Carr.)
Authors: Takahashi Yusuke , Ishiguri Futoshi , Takashima Yuya , Hiraoka Yuichiro , Iki Taiichi , Miyashita Hisaya , Matsushita Michinari , Ohshima Jyunichi , Yokota Shinso
Published: January 2023

Key message

Larger differences of maximum load among families were found in mature wood compared to juvenile wood, suggesting the possibility of improving mature wood with higher resistance to rupture and maintaining characteristics of material in Larix kaempferi (Lamb.) Carr. by selecting specific mating parents.

Context

Because the wood from L. kaempferi trees is used for construction lumber, wood properties and bending properties should be focused on as targeted traits of tree breeding programs.

Aims

We clarified the radial variation of inheritance for wood properties and bending properties and classified the features of bending properties among families in L. kaempferi.

Methods

Annual ring width, latewood percentage, air-dry density, microfibril angle, modulus of elasticity, modulus of rupture, and bending work at five radial positions were investigated for 15 full-sib families of 36-year-old L. kaempferi grown in two progeny test sites in Japan.

Results

Higher heritability at almost all radial positions was found in air-dry density. Phenotypic and genetic correlations between air-dry density and bending properties showed relatively higher values at almost all radial positions. Load-deflection curves in 15 families could be divided into three groups in juvenile and mature wood. Variation among groups for load-deflection curves in mature wood was relatively larger than that in juvenile wood.

Conclusion

Air-dry density can be used as a criterion to select trees with superior bending properties. Mature wood in L. kaempferi could be effectively improved by selecting individuals.
Technical opportunities and challenges in developing total-body PET scanners for mice and rats
Authors: Du Junwei , Jones Terry
Published: January 2023

Positron emission tomography (PET) is the most sensitive in vivo molecular imaging technique available. Small animal PET has been widely used in studying pharmaceutical biodistribution and disease progression over time by imaging a wide range of biological processes. However, it remains true that almost all small animal PET studies using mouse or rat as preclinical models are either limited by the spatial resolution or the sensitivity (especially for dynamic studies), or both, reducing the quantitative accuracy and quantitative precision of the results. Total-body small animal PET scanners, which have axial lengths longer than the nose-to-anus length of the mouse/rat and can provide high sensitivity across the entire body of mouse/rat, can realize new opportunities for small animal PET. This article aims to discuss the technical opportunities and challenges in developing total-body small animal PET scanners for mice and rats.
STRA6 Promotes Thyroid Carcinoma Progression via Activation of the ILK/AKT/mTOR Axis in Cells and Female Nude Mice
Authors: He Weiman , Cheng Zhen , Huo Zijun , Lin Bo , Wang Xuejie , Sun Yijia , Yu Shuang , Cao Siting , Xue Junyu , Liu Rengyun , Lv Weiming , Li Yanbing , Hong Shubin , Xiao Haipeng
Published: January 2023

Background

Metastasis has emerged to be an important cause for poor prognosis of thyroid carcinoma (TC) and its molecular mechanisms are not fully understood. STRA6 is a multifunctional membrane protein widely expressed in embryonic and adult tissues. The function and mechanism of STRA6 in TC remain elusive.

Objective

We aimed to explore the role of STRA6 in TC progression and provide a therapeutic target for TC.

Methods

The expression and clinicopathological relevance of STRA6 were explored in TC. Stable STRA6-knockdown TC cells were established and used to determine the biological function of STRA6 in vitro and in vivo. RNA sequencing and co-immunoprecipitation were performed to unveil the molecular mechanism of STRA6 in TC progression. The potential of STRA6 as a therapeutic target was evaluated by lipid nanoparticles (LNPs) containing siRNA.

Results

STRA6 was upregulated in TC and correlated with aggressive clinicopathological features, including extrathyroidal extension and lymph node metastasis, which contributed to the poor prognosis of TC. STRA6 facilitated TC progression by enhancing proliferation and metastasis in vitro and in vivo. Mechanistically, STRA6 could interact with integrin-linked kinase (ILK) and subsequently activate the protein kinase B/mechanistic target of rapamycin (AKT/mTOR) signaling pathway. We further unveiled that STRA6 reprogrammed lipid metabolism through SREBP1, which was crucial for the metastasis of TC. Moreover, STRA6 siRNA delivered by LNPs significantly inhibited cell growth in xenograft tumor models.

Conclusions

Our study demonstrates the critical roles of STRA6 contributing to TC progression via the ILK/AKT/mTOR axis, which may provide a novel prognostic marker as well as a promising therapeutic target for aggressive TC.