bluuebunny/crossref_metadata_2025 huggingface dataset:
Insect wood borers on commercial North American tree species growing in China: review of Chinese peer-review and grey literature
Authors: Dong Yiyi , Gao Jie , Hulcr Jiri
Published: April 2023

Invasive insect wood borers are a threat to global forests and tree-related industries as they can damage trees and spread plant pathogens. Reports of damages by wood borers on plants that were planted overseas may facilitate the identification of potential invaders and speed up risk assessment. However, much of this information remains unavailable to the international plant protection community due to language barriers, lack of digitization, or limited circulation of regional literature. Here, we investigated reports of wood borers on 7 important North American commercial tree species planted in China (Carya illinoinensis, Liquidambar styraciflua, Pinus elliottii, Pinus taeda, Quercus texana, Quercus rubra, and Quercus virginiana) in peer-reviewed as well as “grey” (nonpeer-reviewed) Chinese literature. A total of 60 unique wood borer records were found, yielding reports of 4 orders, 39 genera, and 44 species of insect wood borers. Among Coleoptera, longhorned beetles (Cerambycidae) were the most commonly reported colonizers of North American trees in China. Chinese peer-reviewed reports of pests on alien plants are a valuable tool to survey for potential wood-boring invaders of North America, and wherever North American trees are planted and have the potential to encounter Asian invasive insects. Digitization and dissemination of non-English literature are essential for contemporary risk assessment. On the other hand, the nonpeer reviewed “grey” literature, primarily agency reports and student theses, provided only 5% of the records; many incidental observations were unreliable.
Cardiac toxicity of chemotherapy for breast cancer: do angiotensin-converting enzyme inhibitors and beta blockers protect?
Authors: Bisceglia Irma , Mistrulli Raffaella , Cartoni Domenico , Matera Sabrina , Petrolati Sandro , Canale Maria Laura
Published: April 2023

Cardiotoxicity is a relatively frequent and potentially serious side effect of anticancer treatments, particularly anthracyclines and trastuzumab, widely used in the treatment of breast cancer. The increase in cancer survivors has generated a growing interest in the prevention of cardiotoxicity. Although early studies suggested an overall benefit on cardiac function with the use of ACE inhibitors (ACEIs) and beta blockers (BBs), more recent randomized trials have demonstrated little or no effect of pharmacological interventions. Even the various meta-analyses conducted in this area have provided weak results in favour of cardioprotective therapies for which the benefit would not always justify the risk of developing side effects. Given the incompleteness of the evidence, there is no clear consensus on which patients should initiate cardioprotective therapy. As recommended in the new guidelines of the European Society of Cardiology, risk stratification before treatment is crucial to identify high-risk patients who would benefit most from the use of cardioprotective therapy. Randomized trials are currently underway to evaluate other therapeutic strategies such as sacubitril/valsartan, and the possibility of using gliflozins in the future cannot be excluded. However, rigorous control and treatment of risk factors remain the primary focus in the management of these patients.
Progress in the treatment of acute pulmonary embolism and chronic thrombo-embolic pulmonary hypertension/disease
Authors: Palazzini Massimiliano , Dardi Fabio , Magnani Ilenia , Guarino Daniele , Galie’ Nazzareno , Manes Alessandra
Published: April 2023

The combination of an initial clinical approach aimed at evaluating the early risk of mortality with subsequent diagnostic and therapeutic approaches articulated on the overall patient’s profile is recommended in acute pulmonary embolism (PE). The presence of pulmonary hypertension associated with the persistence of chronic vascular obstructions in the pulmonary arteries after one or more acute thrombo-embolic events identifies a condition defined as chronic thrombo-embolic pulmonary hypertension (CTEPH). The evolution of technology and knowledge in the field of imaging has allowed us to qualify the computed tomography angiography of the pulmonary arteries as the gold standard for the diagnostic confirmation of both acute PE and CTEPH. In both these conditions, the first therapeutic step is the immediate initiation of anticoagulant therapy. In acute high-risk PE, in addition to anticoagulant therapy, thrombolytic therapy is recommended; in the event of contraindications to thrombolysis, surgical embolectomy or percutaneous catheter-directed treatment represents viable treatment options. In CTEPH, the combination of data collected from cardiac catheterization, computed tomography angiography, and conventional angiography of pulmonary arteries allows a team of experts to identify candidates for pulmonary endarterectomy surgery. Inoperable patients should be considered for percutaneous balloon angioplasty of the pulmonary arteries which can improve patients’ symptoms, quality of life, and prognosis.
The temptation of anticoagulant therapy after transcatheter aortic valve implantation
Authors: Gatto Laura , Scalia Lorenzo
Published: April 2023

The choice of the best antithrombotic strategy after transcatheter aortic valve implantation (TAVI) must be based on the careful balance between the ischaemic risk and the bleeding risk and on the evaluation of some concomitant conditions, such as atrial fibrillation or coronary artery disease which may lead to the choice of anticoagulant treatment or antiplatelet therapy. Another element to consider is the possibility, albeit remote in post-TAVI patients, of thrombosis of the valve leaflets, an event whose clinical impact has yet to be fully clarified and which however appears to present a lower incidence in patients treated with anticoagulants. Recent evidence has shown that in patients who do not require anticoagulant therapy, single therapy with aspirin represents the best treatment compared to dual antiplatelet or to the addition of anticoagulant which in post-TAVI patients should be reserved only for those with a clear indication such as atrial fibrillation. It is still much debated whether in this case the choice should fall on vitamin K antagonists or on the new direct-acting anticoagulants, as the comparison studies have produced inconclusive results.
Treatment of amyloidosis: present and future
Authors: Mallus Maria Teresa , Rizzello Vittoria
Published: April 2023

Cardiac amyloidosis (CA) is an infiltrative heart disease resulting from the deposition of amyloid fibrils in the interstitial spaces of the myocardium. The two main forms of CA are represented by light chain amyloidosis (AL) and transthyretin amyloidosis (ATTR) in the two forms familial or variant or wild-type or senile. Although considered a rare disease, CA is an underdiagnosed disease. Delay in diagnosis has a negative impact on the prognosis, delaying the initiation of specific therapy. The treatment of both forms of CA is based on: (i) prevention and slowing of the generation and deposition of amyloid fibrils and (ii) supportive care of complications. The main success of recent years has been the development of effective therapies that have been possible thanks to the understanding of the pathophysiology of amyloidosis. For the AL form, new therapeutic combinations between a proteasome inhibitor and a monoclonal antibody have been developed. For ATTR forms, the main strategies are transthyretin (TTR) production ‘silencers’ and TTR tetramer stabilizers. Supportive care of patients with CA involves various clinical aspects including treatment of heart failure, arrhythmias, conduction disturbances, thrombo-embolism, and the concomitant presence of aortic stenosis.
Indications, evidence, and controversy in the closure of the left atrial appendage
Authors: Patti Giuseppe , Ghiglieno Chiara
Published: April 2023

Closure of the left atrial appendage (LAAO) represents a valid option for the prevention of cardio-embolic stroke in patients with atrial fibrillation (AF) at high bleeding risk. Previous studies had shown that the atrial appendage represents the site of atrial thrombus formation in about 90% of cases in the presence of non-valvular AF. In all patients with AF and higher thromboembolic risk (in particular with CHA2DS2VASc score ≥2 in women and ≥1 in men) there is an indication for thromboembolic prophylaxis with AOC (oral anti-coagulants). The main guidelines and international consensus documents place the indication for the LAAO in patients with the need for thromboembolic prophylaxis who have contraindications to oral anticoagulant therapy (class of recommendation IIb).
A focus on the percutaneous therapy of mitral and tricuspid regurgitation
Authors: Frazzetto Marco , Sanfilippo Claudio , Ferrarotto Luigi , Tamburino Corrado
Published: April 2023

While mitral stenosis of rheumatic origin has been effectively treated percutaneously for more than 20 years, transcatheter treatment of mitral (MR) and tricuspid (TR) regurgitation appears as a contemporary unmet clinical need. The advent of new transcatheter therapies offers several treatment options for elderly and frail patients at high surgical risk. MitraClip is now consolidated as a therapy for functional MR in selected patients. Transcatheter mitral valve replacement is a promising alternative to transcatheter repair, for both functional and degenerative forms. However, further developments and new evidence are needed. Transcatheter treatment of the tricuspid valve has arrived late compared to similar technologies that have been developed for the aortic and mitral valve, and is currently in its infancy. This is likely due, in part, to the previously underreported impact of TR on patient outcomes. Edge-to-edge repair is the most advanced transcatheter solution in development. Data on annuloplasty and tricuspid valve replacement are limited and more evidence is needed. The future looks promising for transcatheter mitral and tricuspid valve therapies, although their place in clinical practice has yet to be clearly defined.
Heart failure therapy: the fifth card
Authors: Balestrieri Giulio , Sciatti Edoardo , D'isa Salvatore , D'elia Emilia , Senni Michele
Published: April 2023

The 2021 European Society of Cardiology guidelines for the diagnosis and treatment of acute and chronic heart failure (HF) have abandoned the sequential approach for optimal drug therapy and propose four drug classes (enzyme inhibitors conversion agents, angiotensin receptor antagonists, beta-blockers, and sodium–glucose cotransporter inhibitors 2) to be initiated and titrated in all patients with an ejection fraction <35%. This new approach offers advantages such as rapid introduction and titration, better tolerability, and early instrumental re-evaluation. In the VICTORIA study, the molecule vericiguat, a soluble guanylate cyclase activator, was shown to reduce the composite outcome of death from cardiovascular causes and first hospitalization for HF in a high-risk population. An additional randomized clinical trial (VICTOR) is ongoing to evaluate the efficacy and safety of vericiguat in a population with HF on optimized therapy and with no recent episodes of stabilization.
Ventricular rate in atrial fibrillation and the risk of heart failure and death
Authors: Westergaard Lucas Malta , Alhakak Amna , Rørth Rasmus , Fosbøl Emil L , Kristensen Søren L , Svendsen Jesper H , Graff Claus , Nielsen Jonas B , Gislason Gunnar H , Køber Lars , Torp-Pedersen Christian , Lee Christina J Y , Weeke Peter E
Published: April 2023

Aims

While clinical trials have suggested that a high ventricular rate is associated with increased risk of heart failure (HF) and mortality, all-comers studies are warranted.

Objective

To assess 1-year risk of new-onset diagnosed HF and all-cause mortality among rate-control treated patients presenting with atrial fibrillation (AF) on an electrocardiogram (ECG) according to ventricular rate.

Methods and results

ECGs recorded at the Copenhagen General Practitioners Laboratory (2001–15) were used to identify patients with AF. Multivariate Cox proportional hazard regression models were used to compare risk of new-onset HF and all-cause mortality after first ECG presenting with AF according to ventricular rate on ECG [<60, 60–79, 80–99, and 100–110, > 110 beats per minute (bpm)]. We identified 7408 patients in treatment with rate control drugs at time of first ECG presenting with AF [median age 78 years (Q1,Q3 = 70–85 years)], 45.8% male, median ventricular rate 83 bpm, (Q1,Q3 = 71–101 bpm)]. During 1-year follow-up, 666 (9.0%) of all patients with AF developed HF and 858 (11.6%) died. Patients with AF ventricular rates 100–110 bpm and >110 bpm had a hazard ratio (HR) of 1.46 (CI: 1.10–1.95) and 2.41 (CI: 1.94–3.00) respectively for new-onset HF, compared with 60–79 bpm. Similarly, patients with AF ventricular rates 100–110 bpm and >110 bpm had a HR of 1.44 (CI: 1.13–1.82) and 1.34 (CI: 1.08–1.65) respectively for all-cause mortality, compared with 60–79 bpm.

Conclusions

Ventricular rates ≥100 bpm among patients presenting with AF on ECG in treatment with rate control drugs were associated with greater risk of both new-onset HF and all-cause mortality.
Do non-drinking youth drink less alcohol in young adulthood or do they catch up? Findings from a Swedish birth cohort
Authors: Larm Peter , Hellström Charlotta , Raninen Jonas , Åslund Cecilia , Nilsson Kent W , Giannotta Fabrizia
Published: April 2023

Background

Alcohol consumption among adolescents has declined considerably during the last two decades. However, it is unknown if these adolescents’ alcohol consumption will remain low as they grow older. To our knowledge, this is one of the first studies that uses longitudinal data to examine if non-drinking adolescents have a lower alcohol consumption in young adulthood or if they catch up.

Methods

A self-report survey was distributed to a birth cohort (n = 794) born in 1997 in a Swedish region when cohort members attended ninth grade (age 14–15 years) in 2012. Responders were divided into non-drinkers and alcohol users and assessed again in their late teens (17–18 years) and young adulthood (20–21 years).

Results

In their late teens (17–18 years), non-drinkers at baseline consumed less alcohol and had a lower probability of harmful use compared with their alcohol-using peers. In young adulthood (20–21 years), these effects disappeared when adjustment was made for covariates. However, a stratified analysis showed that non-drinking adolescents low in conduct problems consumed less alcohol and had a lower probability of harmful use in young adulthood than alcohol-using peers.

Conclusions

This study suggests that the decline in alcohol use among adolescents in the past decades may be associated with a lower alcohol consumption in the late teens and young adulthood among those low in conduct problems. This may have promising implications for alcohol-related morbidity and mortality.
Peripheral artery disease (PAD) in primary care—educational experiences for PAD primary care in England—a mixed-method study
Authors: Bridgwood Bernadeta M , Sayers Rob D
Published: April 2023

Background

Peripheral artery disease (PAD), the pathophysiologic narrowing of arterial blood vessels of the lower leg due to atherosclerosis, is a highly prevalent disease, with sharp increases in prevalence with age. Primary care is ideally located to identify and manage PAD.

Objectives

This study aims to identify the educational experiences, opinions, and confidence of primary care clinicians (PCCs) regarding PAD.

Method

This mixed-method study was conducted within primary care in England. An online survey was completed with follow-on semistructured interviews, between January and September 2021, with PCCs, namely GPs, practice nurses, and allied professionals (survey n = 874, interviews n = 50).

Results

PCCs report variation in PAD education received, where the content could not often be recalled. Patient-focussed experiential and self-directed learning, formed the largest method to gain PAD education. All PCCs recognized that they have an important role in recognizing PAD yet confidence in recognizing and diagnosing PAD was lacking. PCCs acknowledged that late or missed PAD diagnosis resulted in significant patient morbidity and mortality. Yet many did not recognize PAD as a common disease.

Conclusion

As “specialist–generalists” with finite resources, education provided to primary care needs to be applicable for the multimorbid patient presentations often seen, utilizing resources available in primary care, with consideration to the time constraints endured.
The Regulatory Mendelian Mutation score for GRCh38
Authors: Schubach Max , Nazaretyan Lusiné , Kircher Martin
Published: April 2023

Background

Genome sequencing efforts for individuals with rare Mendelian disease have increased the research focus on the noncoding genome and the clinical need for methods that prioritize potentially disease causal noncoding variants. Some tools for assessment of variant pathogenicity as well as annotations are not available for the current human genome build (GRCh38), for which the adoption in databases, software, and pipelines was slow.

Results

Here, we present an updated version of the Regulatory Mendelian Mutation (ReMM) score, retrained on features and variants derived from the GRCh38 genome build. Like its GRCh37 version, it achieves good performance on its highly imbalanced data. To improve accessibility and provide users with a toolbox to score their variant files and look up scores in the genome, we developed a website and API for easy score lookup.

Conclusions

Scores of the GRCh38 genome build are highly correlated to the prior release with a performance increase due to the better coverage of features. For prioritization of noncoding mutations in imbalanced datasets, the ReMM score performed much better than other variation scores. Prescored whole-genome files of GRCh37 and GRCh38 genome builds are cited in the article and the website; UCSC genome browser tracks, and an API are available at https://remm.bihealth.org.
Political economy analysis of subnational health management in Kenya, Malawi and Uganda
Authors: Rodríguez Daniela C , Balaji Lakshmi Narasimhan , Chamdimba Elita , Kafumba Juba , Koon Adam D , Mazalale Jacob , Mkombe Dadirai , Munywoki Joshua , Mwase-Vuma Tawonga , Namakula Justine , Nambiar Bejoy , Neel Abigail H , Nsabagasani Xavier , Paina Ligia , Rogers Braeden , Tsoka Maxton , Waweru Evelyn , Munthali Alister , Ssengooba Freddie , Tsofa Benjamin
Published: April 2023

The need to bolster primary health care (PHC) to achieve the Sustainable Development Goal (SDG) targets for health is well recognized. In Eastern and Southern Africa, where governments have progressively decentralized health decision-making, health management is critical to PHC performance. While investments in health management capacity are important, so is improving the environment in which managers operate. Governance arrangements, management systems and power dynamics of actors can have a significant influence on health managers’ ability to improve PHC access and quality. We conducted a problem-driven political economy analysis (PEA) in Kenya, Malawi and Uganda to explore local decision-making environments and how they affect management and governance practices for health. This PEA used document review and key informant interviews (N = 112) with government actors, development partners and civil societies in three districts or counties in each country (N = 9). We found that while decentralization should improve PHC by supporting better decisions in line with local priorities from community input, it has been accompanied by thick bureaucracy, path-dependent and underfunded budgets that result in trade-offs and unfulfilled plans, management support systems that are less aligned to local priorities, weak accountability between local government and development partners, uneven community engagement and insufficient public administration capacity to negotiate these challenges. Emergent findings suggest that coronavirus disease 2019 (COVID-19) not only resulted in greater pressures on health teams and budgets but also improved relations with central government related to better communication and flexible funding, offering some lessons. Without addressing the disconnection between the vision for decentralization and the reality of health managers mired in unhelpful processes and politics, delivering on PHC and universal health coverage goals and the SDG agenda will remain out of reach.
Traveling edge states in massive Dirac equations along slowly varying edges
Authors: Hu Pipi , Xie Peng , Zhu Yi
Published: April 2023

Topologically protected wave motion has attracted considerable research interest due to its chirality and potential applications in many applied fields. We construct quasi-traveling wave solutions to the 2D Dirac equation with a domain wall mass in this work. It is known that the system admits exact and explicit traveling wave solutions, which are termed edge states if the interface is a straight line. By modifying such explicit solutions, we construct quasi-traveling-wave solutions if the interface is nearly straight. The approximate solutions in two scenarios are given. One is the circular edge with a large radius, and the second is a straight line edge with the slowly varying along the perpendicular direction. We show the quasi-traveling wave solutions are valid in a long lifespan by energy estimates. Numerical simulations are provided to support our analysis both qualitatively and quantitatively.
Political Institutions and Global Project Finance Loans
Authors: Hong Ji Yeon , Lai Ruilin , Karaca Ilker
Published: April 2023

This paper explores the link between political institutions and the size of global bank loans received to fund project finance (PF) transactions, a commonly used funding method for domestic infrastructure construction. We theorize that lenders’ political risk assessments lead to a prioritization of political predictability over other institutional features of host countries. This indicates that, all else being equal, full democracies and politically closed regimes have advantages in attracting global PF capital, while hybrid regimes are least likely to receive global funds for similar projects. Using the global PF deals reported by the DealScan database and data on political institutions and economic indicators, we show that the relationship between host country regime type and global bank loans is indeed U-shaped. Our additional analysis demonstrates that a greater PF investment is associated with a lower hazard rate of regime failure in host autocracies, particularly in the form of an irregular exit by a dictator. Overall, our findings suggest that global bank loans, mostly originating from economically advanced democracies, contribute to infrastructure funding in dictatorships that might not otherwise be available through domestic or sovereign borrowing channels.
Isolation and characterization of a lipopeptide-producing Bacillus sp. strain JK08 with antagonistic activity against Vibrio parahaemolyticus
Authors: Lv Ran , Li Bin , Xiao Ying , Zhang Jingwen , Mai Yuliang , Hu Xiaoyong , Chen Jiazhi
Published: April 2023

Aims

The present study aimed to isolate a potential antagonist Bacillus sp. and evaluate its capacity for controlling pathogenic Vibrio parahaemolyticus in aquaculture.

Methods and results

Strain JK08, which showed inhibitory activity against V. parahaemolyticus VP02r, was isolated from a Penaeus vannamei pond. Based on morphological, physiological, and biochemical characteristics and phylogenetic analysis, strain JK08 was identified as Bacillus sp. Through culture condition optimization, the maximal inhibition zone diameter (18.19 ± 0.16 mm) was observed when strain JK08 was cultivated at a temperature of 30°C, pH of 7, and salinity of 20‰ in Luria–Bertani broth for 24 h. The inhibition zone against V. parahaemolyticus VP02r of strain JK08 (∼7 μg, in mass of crude antimicrobial substance, per tablet) was larger than those (14–18 mm in diameter) of several commercial antibiotics (10 μg per tablet) in the in vitro antagonism assay. Liquid chromatography–tandem mass spectrometry analysis results indicated the presence of three families of lipopeptides in the antimicrobial substance: surfactin (C12–C17), iturin A (C14–C17), and fengycin A (C14–C17) and B (C17), which might be the key components contributing to the antagonistic activity of strain JK08.

Conclusions

Strain JK08, which is capable of producing antibacterial lipopeptides, shows effective antagonistic activity against V. parahaemolyticus VP02r, implying its promising potential for V. parahaemolyticus control in aquaculture.
Automatic extraction of social determinants of health from medical notes of chronic lower back pain patients
Authors: Lituiev Dmytro S , Lacar Benjamin , Pak Sang , Abramowitsch Peter L , De Marchis Emilia H , Peterson Thomas A
Published: April 2023

Objective

We applied natural language processing and inference methods to extract social determinants of health (SDoH) information from clinical notes of patients with chronic low back pain (cLBP) to enhance future analyses of the associations between SDoH disparities and cLBP outcomes.

Materials and Methods

Clinical notes for patients with cLBP were annotated for 7 SDoH domains, as well as depression, anxiety, and pain scores, resulting in 626 notes with at least one annotated entity for 364 patients. We used a 2-tier taxonomy with these 10 first-level classes (domains) and 52 second-level classes. We developed and validated named entity recognition (NER) systems based on both rule-based and machine learning approaches and validated an entailment model.

Results

Annotators achieved a high interrater agreement (Cohen’s kappa of 95.3% at document level). A rule-based system (cTAKES), RoBERTa NER, and a hybrid model (combining rules and logistic regression) achieved performance of F1 = 47.1%, 84.4%, and 80.3%, respectively, for first-level classes.

Discussion

While the hybrid model had a lower F1 performance, it matched or outperformed RoBERTa NER model in terms of recall and had lower computational requirements. Applying an untuned RoBERTa entailment model, we detected many challenging wordings missed by NER systems. Still, the entailment model may be sensitive to hypothesis wording.

Conclusion

This study developed a corpus of annotated clinical notes covering a broad spectrum of SDoH classes. This corpus provides a basis for training machine learning models and serves as a benchmark for predictive models for NER for SDoH and knowledge extraction from clinical texts.
Clinical Investigation of a Rapid Non-invasive Multispectral Imaging Device Utilizing an Artificial Intelligence Algorithm for Improved Burn Assessment
Authors: Thatcher Jeffrey E , Yi Faliu , Nussbaum Amy E , DiMaio John Michael , Dwight Jason , Plant Kevin , Carter Jeffrey E , Holmes James H
Published: April 2023

Currently, the incorrect judgment of burn depth remains common even among experienced surgeons. Contributing to this problem are change in burn appearance throughout the first week requiring periodic evaluation until a confident diagnosis can be made. To overcome these issues, we investigated the feasibility of an artificial intelligence algorithm trained with multispectral images of burn injuries to predict burn depth rapidly and accurately, including burns of indeterminate depth. In a feasibility study, 406 multispectral images of burns were collected within 72 hours of injury and then serially for up to 7 days. Simultaneously, the subject’s clinician indicated whether the burn was of indeterminate depth. The final depth of burned regions within images were agreed upon by a panel of burn practitioners using biopsies and 21-day healing assessments as reference standards. We compared three convolutional neural network architectures and an ensemble in their capability to automatically highlight areas of nonhealing burn regions within images. The top algorithm was the ensemble with 81% sensitivity, 100% specificity, and 97% positive predictive value (PPV). Its sensitivity and PPV were found to increase in a sigmoid shape during the first week postburn, with the inflection point at day 2.5. Additionally, when burns were labeled as indeterminate, the algorithm’s sensitivity, specificity, PPV, and negative predictive value were: 70%, 100%, 97%, and 100%. These results suggest multispectral imaging combined with artificial intelligence is feasible for detecting nonhealing burn tissue and could play an important role in aiding the earlier diagnosis of indeterminate burns.
A survey of the genes encoding trehalose-metabolism enzymes in crustaceans
Authors: Huang Yuting , Shi Qiong
Published: April 2023

Trehalose is important in activity, development, and environmental-stress response, especially in invertebrates. It is mainly synthesized by trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP), and degraded by trehalase (TRE). In the present study, the tps, tpp, and tre were identified from various crustacean species and their phylogeny, structure, network, and transcriptome were analyzed. The tps and tpp are fused in crustaceans, accompanied with multi-copies of genes to improve the synthesis capacity of trehalose, and they may be formed by whole-genome duplication (WGD) and/or segmental duplications. Phylogenetic subgroups of enzymes in the same species may be due to the different lengths and distribution positions of domains. The protein with single TPP domain in the salmon louse, the copepod Lepeophtheirus salmonis (Krøyer, 1837), probably has a depoisoning effect. Structure analyses and location predictions showed that crustacean TRE possess an α-helix-rich structure with barrel core, and are membrane-bound, cytoplasmic, and secreted. Additionally, the non-acid TRE might not be adjusted by Ca2+ because there is no binding domain in crustaceans. Expression profiles of different tissues, developmental periods, and environmental-challenge responses, as well as genes of co-expression networks suggested that TPS (including TPP) and TRE might play important roles in physiological activities including development and environmental adaptation in crustaceans. Multi-copies of tre may enhance survival ability of copepods in diverse and sometimes harsh environments. Branchiopods, copepods, and the marine shrimp Penaeus vannamei Boone, 1931 are suspected to adopt possible acid TRE as a supplementary strategy in response to stress.
The CJEU sets aside the quadruple fee rule for plant variety right infringers covered by the ‘agricultural exemption’
Authors: Crespo Velasco Adrián
Published: April 2023

The Court of Justice of the European Union (CJEU) has invalidated Article 18(2) of Regulation No 1768/95, setting aside the ‘quadruple fee rule’ for intentional and negligent plant variety right infringers covered by the ‘agricultural exemption’.
The role of chloroplast movement in C4 photosynthesis: a theoretical analysis using a three-dimensional reaction–diffusion model for maize
Authors: Retta Moges A , Yin Xinyou , Ho Quang Tri , Watté Rodrigo , Berghuijs Herman N C , Verboven Pieter , Saeys Wouter , Cano Francisco Javier , Ghannoum Oula , Struik Paul C , Nicolaï Bart M
Published: April 2023

Chloroplasts movement within mesophyll cells in C4 plants is hypothesized to enhance the CO2 concentrating mechanism, but this is difficult to verify experimentally. A three-dimensional (3D) leaf model can help analyse how chloroplast movement influences the operation of the CO2 concentrating mechanism. The first volumetric reaction–diffusion model of C4 photosynthesis that incorporates detailed 3D leaf anatomy, light propagation, ATP and NADPH production, and CO2, O2 and bicarbonate concentration driven by diffusional and assimilation/emission processes was developed. It was implemented for maize leaves to simulate various chloroplast movement scenarios within mesophyll cells: the movement of all mesophyll chloroplasts towards bundle sheath cells (aggregative movement) and movement of only those of interveinal mesophyll cells towards bundle sheath cells (avoidance movement). Light absorbed by bundle sheath chloroplasts relative to mesophyll chloroplasts increased in both cases. Avoidance movement decreased light absorption by mesophyll chloroplasts considerably. Consequently, total ATP and NADPH production and net photosynthetic rate increased for aggregative movement and decreased for avoidance movement compared with the default case of no chloroplast movement at high light intensities. Leakiness increased in both chloroplast movement scenarios due to the imbalance in energy production and demand in mesophyll and bundle sheath cells. These results suggest the need to design strategies for coordinated increases in electron transport and Rubisco activities for an efficient CO2 concentrating mechanism at very high light intensities.
Quantitative, Spectro-kinetic Analysis of Oxygen in Electron-Beam Sensitive, Multimetallic Oxide Nanostructures
Authors: López-Haro Miguel , Gómez-Recio Isabel , Pan Huiyan , Delgado Juan J , Chen Xiaowei , Cauqui Miguel A , Pérez-Omil José A , Ruiz-González María L , Hernando María , Parras Marina , González-Calbet José M , Calvino José J
Published: April 2023

The oxygen stoichiometry of hollandite, KxMnO2-δ, nanorods has been accurately determined from a quantitative analysis of scanning-transmission electron microscopy (STEM) X-Ray Energy Dispersive Spectroscopy (XEDS) experiments carried out in chrono-spectroscopy mode. A methodology combining 3D reconstructions of high-angle annular dark field electron tomography experiments, using compressed-sensing algorithms, and quantification through the so-called ζ-factors method of XEDS spectra recorded on a high-sensitivity detector has been devised to determine the time evolution of the oxygen content of nanostructures of electron-beam sensitive oxides. Kinetic modeling of O-stoichiometry data provided K0.13MnO1.98 as overall composition for nanorods of the hollandite. The quantitative agreement, within a 1% mol error, observed with results obtained by macroscopic techniques (temperature-programmed reduction and neutron diffraction) validate the proposed methodology for the quantitative analysis, at the nanoscale, of light elements, as it is the case of oxygen, in the presence of heavy ones (K, Mn) in the highly compromised case of nanostructured materials which are prone to electron-beam reduction. Moreover, quantitative comparison of oxygen evolution data measured at macroscopic and nanoscopic levels allowed us to rationalize beam damage effects in structural terms and clarify the exact nature of the different steps involved in the reduction of these oxides with hydrogen.
CSS1603+19: a low-mass polar near the cataclysmic variable period minimum
Authors: Liu Yiqi , Hwang Hsiang-Chih , Zakamska Nadia L , Thorstensen John R
Published: April 2023

CSS1603+19 is a cataclysmic variable (CV) with an orbital period of 81.96 min, near the minimal period of CVs. It is unusual in having a strong mid-infrared excess inconsistent with thermal emission from a brown dwarf companion. Here, we present time-resolved multiwavelength observations of this system. WISE photometry indicates that the mid-infrared excess displays a one-magnitude eclipsing-like variability during the orbit. We obtained near-infrared and optical spectroscopy using Gemini, MDM, and APO telescopes. Near-infrared spectra show possible cyclotron features indicating that the white dwarf has a magnetic field of about 5 MG. Optical and near-infrared spectra display double-peaked emission lines, with both components showing strong radial velocity variations during the orbital period and with the broad component leading the narrow component stably by about 0.2 of the orbital phase. We construct a physical model informed by existing observations of the system and determine that one component likely originates from the accretion column on to the magnetized white dwarf in synchronous rotation with the orbital motion and the other from the Roche overflow point. This allows us to constrain the masses of the binary components to be M1 > 0.24 M⊙ for the white dwarf accretor and M2 = 0.0644 ± 0.0074 M⊙ for the donor. We classify the system as an AM Herculis star, or a polar. It has likely completed its stint on the period gap, but has not yet gone through the period bounce.
Support for the standard picture of thermal X-rays in the wind of ζ Puppis from dielectronic recombination of He-like ions
Authors: Gunderson Sean J , Gayley Kenneth G , Huenemoerder David P
Published: April 2023

We use the line ratios of resolved X-ray resonance and satellite line pairs from Si xiii and Mg xi ions to test the widely held assumption that the shocked plasma in the wind of hot stars is in collisional ionization equilibrium. We specifically apply this test to the prototypical O supergiant ζ Puppis, as its line ratios are well resolved in its total 813 ks Chandra observation. This satellite-to-resonance line ratio is known to be a reliable diagnostic of plasma temperature but has yet to be applied to hot stars due to needing a long exposure time to see both lines above the continuum. In testing the assumed plasma state, we also provide evidence against significant ionization and excitation from proton collisions. Since dielectronic recombination (DR) allows somewhat lower energy free electrons to excite resonance lines than by the usual collisional pathways, we also use the DR flux ratios to extend diagnostics of the ionization fraction of the parent ion to lower temperatures than any other method can achieve. Also, the DR ratio supports a concept of an average free electron temperature, for each target ion used, which can constrain heating models.
Dissect two-halo galactic conformity effect for central galaxies: the dependence of star formation activities on the large-scale environment
Authors: Wang Kai , Peng Yingjie , Chen Yangyao
Published: April 2023

We investigate the two-halo galactic conformity effect for central galaxies, which is the spatial correlation of the star formation activities for central galaxies to several Mpcs, by studying the dependence of the star formation activities of central galaxies on their large-scale structure in our local Universe using the SDSS data. Here we adopt a novel environment metric using only central galaxies quantified by the distance to the nth nearest central galaxy. This metric measures the environment within an aperture from ∼1 to ≳ 10 Mpc, with a median value of ∼4 Mpc. We found that two kinds of conformity effects in our local Universe. The first one is that low-mass central galaxies are more quenched in high-density regions, and we found that this effect mainly comes from low-mass centrals that are close to a more massive halo. A similar trend is also found in the IllustrisTNG simulation, which can be entirely explained by backsplash galaxies. The second conformity effect is that massive central galaxies in low-density regions are more star-forming. This population of galaxies also possesses a higher fraction of spiral morphology and lower central stellar velocity dispersion, suggesting that their low quiescent fraction is due to less-frequent major merger events experienced in the low-density regions and, as a consequence, less-massive bulges and central black holes.
FAM129A promotes self-renewal and maintains invasive status via stabilizing the Notch intracellular domain in glioma stem cells
Authors: Liu Guohao , Zhang Po , Chen Sui , Chen Zirong , Qiu Yanmei , Peng Peng , Huang Wenda , Cheng Fangling , Zhang Yang , Li Huan , Xiao Qungen , Mao Feng , Wang Baofeng , Jiang Xiaobing , Wan Feng , Guo Dongsheng , Yu Xingjiang
Published: April 2023

Background

Glioma stem cells (GSCs) are a subpopulation of tumor cells with self-renewal and tumorigenic capabilities in glioblastomas (GBMs). Diffuse infiltration of GSCs facilitates tumor progression and frustrates efforts at effective treatment. Further compounding this situation is the currently limited understanding of what drives GSC invasion. Here we comprehensively evaluated the significance of a novel invasion-related protein, Family with Sequence Similarity 129 Member A (FAM129A), in infiltrative GSCs.

Methods

Western blotting, immunohistochemistry, and gene expression analysis were used to quantify FAM129A in glioma specimens and cancer datasets. Overexpression and knockdown of FAM129A in GSCs were used to investigate its effects on tumor growth and invasion. RNA-seq, qRT-PCR, western blotting, and co-precipitation assays were used to investigate FAM129A signaling mechanisms.

Results

FAM129A is preferentially expressed in invasive frontiers. Targeting FAM129A impairs GSC invasion and self-renewal. Mechanistically, FAM129A acted as a positive regulator of Notch signaling by binding with the Notch1 intracellular domain (NICD1) and preventing its degradation.

Conclusions

FAM129A and NICD1 provide a precise indicator for identifying tumor margins and aiding prognosis. Targeting them may provide a significantly therapeutic strategy for GSCs.
Emerging open-channel droplet arrays for biosensing
Authors: Song Yongchao , Wang Lirong , Xu Tailin , Zhang Guangyao , Zhang Xueji
Published: April 2023

Open-channel droplet arrays have attracted much attention in the fields of biochemical analysis, biofluid monitoring, biomarker recognition and cell interactions, as they have advantages with regard to miniaturization, parallelization, high-throughput, simplicity and accessibility. Such droplet arrays not only improve the sensitivity and accuracy of a biosensor, but also do not require sophisticated equipment or tedious processes, showing great potential in next-generation miniaturized sensing platforms. This review summarizes typical examples of open-channel microdroplet arrays and focuses on diversified biosensing integrated with multiple signal-output approaches (fluorescence, colorimetric, surface-enhanced Raman scattering (SERS), electrochemical, etc.). The limitations and development prospects of open-channel droplet arrays in biosensing are also discussed with regard to the increasing demand for biosensors.
Fibreglass dermatitis: diagnostic challenges and occupational interventions
Authors: Lim S Y D , Teo S , Tan K W A , Cheng W N S
Published: April 2023

Fibreglass dermatitis is a common occupationally acquired irritant contact dermatitis, where small spicules of fibreglass lodging in the stratum corneum result in mechanical irritation. We present two patients, an air-conditioning ducting worker and an injection moulding machine operator, who both presented with generalized pruritus. In the first case, polarized microscopy of a skin biopsy specimen demonstrated rare small spicules, with a diameter of 1 µm, lodged in the stratum corneum. In the second case, skin tape stripping demonstrated fibreglass particles, not found on skin biopsy. Proper work practices, personal hygiene and use of impervious barrier materials were recommended. The first patient did not return for follow-up, and the second patient’s dermatitis resolved after handling of fibreglass-containing material was eliminated from his job scope. In conclusion, we present two cases of fibreglass dermatitis to illustrate the challenges in diagnosis and highlight strategies for prevention.
Latent Tuberculosis Infection Treatment Practices in Two Large Integrated Health Systems in California, 2009–2018
Authors: Bruxvoort Katia J , Skarbinski Jacek , Fischer Heidi , Li Zhuoxin , Eaton Abigail , Qian Lei , Spence Brigitte , Wei Rong , Rieg Gunter , Shaw Sally , Tartof Sara Y
Published: April 2023

Background

Treatment of latent tuberculosis infection (LTBI) is highly effective at preventing active tuberculosis (TB) disease. Understanding LTBI treatment practices in US health system settings is critical to identify opportunities to improve treatment prescription, initiation, and completion, and thus to prevent TB disease.

Methods

We assessed LTBI treatment practices among a cohort of adults after their first positive LTBI test (tuberculin skin test [TST] or interferon gamma release assay [IGRA]) between 2009 and 2018 at 2 large integrated health systems in California. We described the prescription, initiation, and completion of LTBI treatment (isoniazid [INH], rifampin, and rifamycin-INH short-course combinations) by demographic and clinical characteristics. We used multivariable robust Poisson regression to examine factors that were independently associated with treatment prescription and completion.

Results

Among 79 302 individuals with a positive LTBI test, 33.0% were prescribed LTBI treatment, 28.3% initiated treatment, and 18.5% completed treatment. Most individuals were prescribed INH (82.0%), but treatment completion was higher among those prescribed rifamycin-INH short-course combinations (69.6% for INH + rifapentine and 70.3% for INH + rifampin) compared with those prescribed INH (56.3%) or rifampin (56.6%). In adjusted analyses, treatment prescription and completion were associated with older age, female sex, more comorbidities, immunosuppression, not being born in a high–TB incidence country, and testing positive with IGRA vs TST.

Conclusions

LTBI treatment is underutilized, requiring tailored interventions to support treatment prescription and completion for patients with LTBI.
The role of coronary computed tomography angiography in pregnancy-associated myocardial infarction
Authors: Chokhachi Reihaneh , Reiss Craig K
Published: April 2023

Background

Pregnancy-associated coronary artery dissection is one of the critical causes of myocardial infarction in young females. The challenges in the diagnosis and follow-up of pregnancy-associated myocardial infarction prompted us to highlight the potential role of coronary computed angiography in managing this life-threatening medical condition.

Case Summary

We describe a case of a 29-year-old female with sudden onset chest discomfort one-week postpartum. She presented to the hospital with an ST-elevation myocardial infarction and was urgently taken to the cath lab for PCI and stent placement. Thirteen months later, with intermittent chest discomfort, an coronary computed tomography was done, demonstrating evidence of SCAD and aneurysm formation in the left main coronary artery and left circumflex coronary artery. Repeat cardiac catheterization confirmed the same diagnosis. Given her refractory chest discomfort and the chance of aneurysm rupture decision was made to proceed with a three-vessel CABG. At 3-month and 1-year followup visits, the patient is doing well and remains pain-free.

Conclusion

In this case presentation, we aim to highlight the potential role of Coronary Computed tomography angiography in the diagnosis and follow-up of pregnancy-associated spontaneous coronary artery dissection, given the high accuracy and non invasiveness of this diagnostic modality in the context of the high prevalence of spontaneous coronary artery dissection in symptomatic or asymptomatic pregnancy-associated myocardial infarction.