bluuebunny/crossref_metadata_2025 huggingface dataset:
Political-economic correlates of environmental policy
*
Authors: Kammerlander Andreas , Schulze Günther G
Published: January 2021

What are the correlates of environmental performance? In particular, does globalization lead to environmental degradation? What is the role of democracy for environmental performance and do left-wing governments really care more about the environment? Using a novel and comprehensive measure of environmental performance, we test these three hypotheses for a panel of 134 countries for the period 2007–2016. Our results are surprising—we find no evidence that democracies are cleaner, left-wing governments perform better than right-wing governments, but centrist governments clearly have the highest environmental performance, and globalization is good for the environment. The positive impact of globalization, however, is driven by social globalization—economic and political globalization do not play a role.
Relationship between two types of heat waves in northern East Asia and temperature anomalies in Eastern Europe
Authors: Yang Xiaoye , Zeng Gang , Zhang Shiyue , Hao Zhixin , Iyakaremye Vedaste
Published: January 2021

In this study, the hierarchical clustering algorithm was used to cluster the summer heat waves (HWs) in northern East Asia (NEA) into wave-train HW and blocking HW. In the past 38 years, the wave-train HWs have increased significantly, while the blocking HWs have not shown a visible trend. The wave-train index and the blocking index are proposed to objectively identify the HW type after capturing the circulation characteristics of two kinds of HWs. When a wave-train HW occurs in NEA, simultaneous HW will occur in Eastern Europe. However, when a blocking HW occurs in NEA, a negative temperature anomaly will take place in Eastern Europe. The results of numerical simulations using the linear baroclinic model indicate that the wave-train HWs and the blocking HWs are related to the heating anomaly over southern Greenland and the cooling anomaly over the Barents Sea, respectively.
How norms, needs, and power in science obstruct transformations towards sustainability
Authors: Lahsen Myanna , Turnhout Esther
Published: January 2021

After decades of inadequate responses to scientists’ warnings about global environmental threats, leading analysts of the science-policy interface are seeking an important shift of research focus. This switch is from continued modeling and diagnoses of biogeochemical conditions in favor of enhanced efforts to understand the many socio-political obstacles to achieving just transformations towards sustainability, and how to overcome them. We discuss why this shift continues to prove elusive. We argue that rarely analyzed mutually reinforcing power structures, interests, needs, and norms within the institutions of global environmental change science obstruct rethinking and reform. The blockage created by these countervailing forces are shielded from scrutiny and change through retreats behind shields of neutrality and objectivity, stoked and legitimated by fears of losing scientific authority. These responses are maladaptive, however, since transparency and reflexivity are essential for rethinking and reform, even in contexts marked by anti-environmentalism. We therefore urge greater openness, self-critique, and power-sharing across research communities, to create spaces and support for conversations, diverse knowledges, and decisions conducive to sustainability transformations.
Cooling hot cities: a systematic and critical review of the numerical modelling literature
Authors: Krayenhoff E Scott , Broadbent Ashley M , Zhao Lei , Georgescu Matei , Middel Ariane , Voogt James A , Martilli Alberto , Sailor David J , Erell Evyatar
Published: January 2021

Infrastructure-based heat reduction strategies can help cities adapt to high temperatures, but simulations of their cooling potential yield widely varying predictions. We systematically review 146 studies from 1987 to 2017 that conduct physically based numerical modelling of urban air temperature reduction resulting from green-blue infrastructure and reflective materials. Studies are grouped into two modelling scales: neighbourhood scale, building-resolving (i.e. microscale); and city scale, neighbourhood-resolving (i.e. mesoscale). Street tree cooling has primarily been assessed at the microscale, whereas mesoscale modelling has favoured reflective roof treatments, which are attributed to model physics limitations at each scale. We develop 25 criteria to assess contextualization and reliability of each study based on metadata reporting and methodological quality, respectively. Studies have shortcomings with respect to neighbourhood characterization, reporting areal coverages of heat mitigation implementations, evaluation of base case simulations, and evaluation of modelled physical processes relevant to heat reduction. To aid comparison among studies, we introduce two metrics: the albedo cooling effectiveness (ACE), and the vegetation cooling effectiveness (VCE). A sub-sample of 47 higher quality studies suggests that high reflectivity coatings or materials offer ≈0.2 °C–0.6 °C cooling per 0.10 neighbourhood albedo increase, and that trees yield ≈0.3 °C cooling per 0.10 canopy cover increase, for afternoon clear-sky summer conditions. VCE of low vegetation and green roofs varies more strongly between studies. Both ACE and VCE exhibit a striking dependence on model choice and model scale, particularly for albedo and roof-level implementations, suggesting that much of the variation of cooling magnitudes between studies may be attributed to model physics representation. We conclude that evaluation of the base case simulation is not a sufficient prerequisite for accurate simulation of heat mitigation strategy cooling. We identify a three-phase framework for assessment of the suitability of a numerical model for a heat mitigation experiment, which emphasizes assessment of urban canopy layer mixing and of the physical processes associated with the heat reduction implementation. Based on our findings, we include recommendations for optimal design and communication of urban heat mitigation simulation studies.
Vulnerability of European wheat to extreme heat and drought around flowering under future climate
Authors: Senapati Nimai , Halford Nigel G , Semenov Mikhail A
Published: January 2021

Identifying the future threats to crop yields from climate change is vital to underpin the continuous production increases needed for global food security. In the present study, the vulnerability of European wheat yield to heat and drought stresses around flowering under climate change was assessed by estimating the 95-percentiles of two indices at flowering under rain-fed conditions: the heat stress index (HSI95) and the drought stress index (DSI95). These two indices represent the relative yield losses due heat stress or drought stress around flowering that could be expected to occur once every 20 years on average. The Sirius wheat model was run under the predicted 2050-climate at 13 selected sites, representing the major wheat-growing regions in Europe. A total of 19 global climate models (GCMs) from the CMIP5 ensemble were used to construct local-scale climate scenarios for 2050 (RCP8.5) by downscaling GCMs climate projections with the LARS-WG weather generator. The mean DSI95 due to extreme drought around flowering under the baseline climate (1981–2010) was large over Europe (DSI95 ∼ 0.28), with wide site variation (DSI95 ∼ 0.0–0.51). A reduction of 12% in the DSI95 was predicted under the 2050-climate; however, vulnerability due to extreme drought around flowering would remain a major constraint to wheat yield (DSI95 ∼ 0–0.57). In contrast, HSI95 under the baseline climate was very small over Europe (HSI95 ∼ 0.0–0.11), but was predicted to increase by 79% (HSI95 ∼ 0.0–0.23) under the 2050-climate, categorising extreme heat stress around flowering as an emergent threat to European wheat production. The development of wheat varieties that are tolerant to drought and heat stresses around flowering, is required, if climate change is not to result in a reduction of wheat yield potential under the future climate in Europe.
Microdynamic and thermodynamic properties of dissipative dust-acoustic solitons
Authors: Trukhachev F M , Vasiliev M M , Petrov O F , Vasilieva E V
Published: January 2021

Properties of weakly dissipative dust-acoustic solitons are analyzed on the basis of the hydrodynamic and single-particle approximation (Lagrangian–Euler approach). Significant differences between dissipative and conservative solitons are found. Particle-wave microdynamic parameters such as trajectories, phase trajectories, and drift velocity of dust particles under the action of a cascade of solitons are calculated. It is shown that dissipation is responsible for the interconnection of solitons in the ensemble. In addition, dissipative solitons significantly affect the environment comparing with conservative ones. The heat release processes that determine the production of entropy are analyzed. The role of dissipation in the ordering of charged particles trajectories is revealed. The theoretical results are in a reasonable agreement with known experimental data.
Vorticity and circulation decay in the viscous Lamb dipole
Authors: Krasny Robert , Xu Ling
Published: January 2021

The Lamb dipole is a steady translating structure in 2D ideal fluid flow with opposite-sign vorticity of compact support in a circular disk. Previous studies have shown that when viscosity is present, the resulting viscous Lamb dipole develops a head-tail structure in which the head expands in size, while a tail of low amplitude vorticity is left behind as the head moves forward; in addition, the maximum vorticity and total circulation on each side of the dipole decay in time. Here we examine these decay properties by comparing numerical solutions of the Navier–Stokes equation (NSE) and diffusion equation (DE) in the Reynolds number range

125
⩽
Re
⩽
1000

using the inviscid Lamb dipole as initial condition; this enables us to compare the combined effects of convection and diffusion in the NSE with the sole effect of diffusion in the DE. The results show that for a given Re, the vortex core size, shape, and maximum vorticity are nearly the same for the NSE and DE, indicating that convection has little effect on these properties. Nonetheless, compared to the DE, convection in the NSE inhibits circulation decay at low Re, while it enhances circulation decay at high Re, and the lateral separation of the vortex cores is a critical factor in this transition.
Effects of pore structure characteristics on performance of sintered bi-porous Ti3AlC2 wicks
Authors: Cao Yingwen , Guo Chunsheng , Wu Dongting , Zou Yong
Published: January 2021

Loop heat pipe, carried out by liquid-gas phase transition, has been attracted as an efficient heat management device in high-heat-flux, long-distance and anti-gravity situations. The capillary pressure provided by the porous wick in the evaporator drives circulation for working liquid, affecting the heat exchange efficiency for loop heat pipes directly. In this work, the bi-porous Ti3AlC2 wick was produced by reaction sintering and pore formers dissolution and porosity and pore size were regulated by varying pore formers content and cold pressure. The pore size distribution was characterized by mercury intrusion and effects on capillary performance and thermal conductivity were also analyzed. The porosity and proportion of large pores increased with the increased pore formers content, causing the enhancement of capillary performance and the reduction of thermal conductivity. As the cold pressure increased, pore size distribution concentrated, the proportion of fine pores increased and the porosity fluctuated slightly, increasing the capillary pressure and improving capillary performance and thermal conductivity. Based on experimental results, the parameter of pore size proportion was proposed to modify the Alexander model to predict the thermal conductivity of porous materials accurately, guide the design for pore structure and promote the transfer capacity.
Fast radio bursts
Authors: Chatterjee Shami
Published: January 2021

Shami Chatterjee reviews fast radio bursts, focusing on the rapid recent progress in observations of these enigmatic events, our understanding of their central engines, and their use as probes of the intergalactic medium
Using single-cell cytometry to illustrate integrated multi-perspective evaluation of clustering algorithms using Pareto fronts
Authors: Putri Givanna H , Koprinska Irena , Ashhurst Thomas M , King Nicholas J C , Read Mark N
Published: January 2021

Motivation

Many ‘automated gating’ algorithms now exist to cluster cytometry and single-cell sequencing data into discrete populations. Comparative algorithm evaluations on benchmark datasets rely either on a single performance metric, or a few metrics considered independently of one another. However, single metrics emphasize different aspects of clustering performance and do not rank clustering solutions in the same order. This underlies the lack of consensus between comparative studies regarding optimal clustering algorithms and undermines the translatability of results onto other non-benchmark datasets.

Results

We propose the Pareto fronts framework as an integrative evaluation protocol, wherein individual metrics are instead leveraged as complementary perspectives. Judged superior are algorithms that provide the best trade-off between the multiple metrics considered simultaneously. This yields a more comprehensive and complete view of clustering performance. Moreover, by broadly and systematically sampling algorithm parameter values using the Latin Hypercube sampling method, our evaluation protocol minimizes (un)fortunate parameter value selections as confounding factors. Furthermore, it reveals how meticulously each algorithm must be tuned in order to obtain good results, vital knowledge for users with novel data. We exemplify the protocol by conducting a comparative study between three clustering algorithms (ChronoClust, FlowSOM and Phenograph) using four common performance metrics applied across four cytometry benchmark datasets. To our knowledge, this is the first time Pareto fronts have been used to evaluate the performance of clustering algorithms in any application domain.

Availability and implementation

Implementation of our Pareto front methodology and all scripts and datasets to reproduce this article are available at https://github.com/ghar1821/ParetoBench.

Supplementary information

Supplementary data are available at Bioinformatics online.
Effect of the SARS-CoV-2 pandemic on mortality related to high-risk emergency and major elective surgery
Authors: Deputy M , Rao C , Worley G , Balinskaite V , Bottle A , Aylin P , Burns E M , Faiz O
Published: January 2021

These data show large reductions in both elective and emergency activity that are concerning for unmeasured morbidity and mortality within the community. The risk of mortality following high-risk EGS and major elective surgery during the first wave of the pandemic did not differ when compared with date-matched patient cohorts from 2019. The prevalence of concomitant SARS-CoV-2 infection in this surgical population is low.
Sleep-Dependent Memory in Older People With and Without MCI: The Relevance of Sleep Microarchitecture, OSA, Hippocampal Subfields, and Episodic Memory
Authors: Lam Aaron , Haroutonian Carla , Grummitt Lucy , Ireland Catriona , Grunstein Ronald R , Duffy Shantel , D’Rozario Angela , Naismith Sharon L
Published: January 2021

This study aimed to determine if, relative to cognitively healthy controls, sleep-dependent memory consolidation (SDMC) is diminished in mild cognitive impairment (MCI), a group at high risk of conversion to dementia. We also sought to determine whether SDMC is associated with sleep characteristics, daytime episodic memory, and hippocampal integrity. Participants with MCI (n = 43) and controls (n = 20) underwent clinical and neuropsychological profiling. From polysomnography, apnea hypopnea index (AHI) and non-REM sleep spindle characteristics were derived. From magnetic resonance imaging, hippocampal subfield volumes were computed. Participants learned a novel 32-item word-pair prior to sleep; morning retention of the word-pairs was used to determine SDMC. Results showed that SDMC did not differ between MCI and controls, but there was a large effect size decrement in SDMC in those with multiple domain MCI (Hedge’s g = 0.85). In MCI, poorer SDMC was correlated with CA1 and CA3 hippocampal atrophy, shorter spindle duration, and worse daytime episodic memory. In controls, poorer SDMC was associated with higher AHI. Impaired daytime memory consolidation, reduced hippocampal volumes, shorter sleep spindles, and greater sleep apnea severity are indicators of diminished SDMC in older adults and should be explored in future studies.
Effective Treatment of Lymphogranuloma venereum Proctitis With Azithromycin
Authors: Blanco José L , Fuertes Irene , Bosch Jordi , De Lazzari Elisa , Gonzalez-Cordón Ana , Vergara Andrea , Blanco-Arevalo Alejandro , Mayans Josep , Inciarte Alexy , Estrach Teresa , Martinez Esteban , Cranston Ross D , Gatell Josep M , Alsina-Gibert Merce
Published: January 2021

Background

Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis (CT) serovars L1, L2, and L3 and is endemic among men who have sex with men (MSM) in Europe. We evaluated weekly oral azithromycin 1 g for 3 weeks as a treatment for LGV proctitis.

Methods

This is an open clinical trial with convenience allocation according to treating physician preferences. Adults with clinical proctitis received a single dose of 1 g of intramuscular ceftriaxone and were subsequently allocated to receive (i) doxycycline 100 mg twice daily for 21 days (Doxycycline group) or (ii) azithromycin 1 g orally once weekly for 3 weeks (Azithromycin group). LGV cure (primary endpoint) was defined as resolution of symptoms at week 6 (clinical cure, LGV-CC), with an additional supporting negative rectal polymerase chain reaction (PCR) at week 4 (microbiological cure, LGV-MC), if available.

Results

One hundred and twenty-five individuals with LGV clinical proctitis were included. All were MSM, and 96% were living with human immunodeficiency virus (HIV). Eighty-two were in the Azithromycin group, and 43 were in the Doxycycline group. LGV cure on a modified intention-to-treat analysis (primary endpoint), occurred in 80 of 82 (98%) in the Azithromycin group versus 41 of 43 (95%) in the Doxycycline group (treatment difference [95% confidence interval {CI}] 2.2% [−3.2, 13.2]). LGV-MC occurred in 70 of 72 (97%) vs 15 of 15 (100%) in the Azithromycin group and Doxycycline group, respectively (treatment difference [95% CI] −2.8% [−9.6; 17.7]). Adverse events were similar in both treatment groups.

Conclusions

Our findings support extended azithromycin dosing as an alternative treatment option for symptomatic LGV proctitis and provides the rationale for future randomized trials.
Effects of genetic background, sex, and age on murine atrial electrophysiology
Authors: Obergassel Julius , O’Reilly Molly , Sommerfeld Laura C , Kabir S Nashitha , O’Shea Christopher , Syeda Fahima , Eckardt Lars , Kirchhof Paulus , Fabritz Larissa
Published: January 2021

Aims

Genetically altered mice are powerful models to investigate mechanisms of atrial arrhythmias, but normal ranges for murine atrial electrophysiology have not been robustly characterized.

Methods and results

We analyzed results from 221 electrophysiological (EP) studies in isolated, Langendorff-perfused hearts of wildtype mice (114 female, 107 male) from 2.5 to 17.7 months (mean 7 months) with different genetic backgrounds (C57BL/6, FVB/N, MF1, 129/Sv, Swiss agouti). Left atrial monophasic action potential duration (LA-APD), interatrial activation time (IA-AT), and atrial effective refractory period (ERP) were summarized at different pacing cycle lengths (PCLs). Factors influencing atrial electrophysiology including genetic background, sex, and age were determined.

LA-APD70 was 18 ± 0.5 ms, atrial ERP was 27 ± 0.8 ms, and IA-AT was 17 ± 0.5 ms at 100 ms PCL. LA-APD was longer with longer PCL (+17% from 80 to 120 ms PCL for APD70), while IA-AT decreased (−7% from 80 to 120 ms PCL). Female sex was associated with longer ERP (+14% vs. males). Genetic background influenced atrial electrophysiology: LA-APD70 (−20% vs. average) and atrial ERP (−25% vs. average) were shorter in Swiss agouti background compared to others. LA-APD70 (+25% vs. average) and IA-AT (+44% vs. average) were longer in 129/Sv mice. Atrial ERP was longer in FVB/N (+34% vs. average) and in younger experimental groups below 6 months of age.

Conclusion

This work defines normal ranges for murine atrial EP parameters. Genetic background has a profound effect on these parameters, at least of the magnitude as those of sex and age. These results can inform the experimental design and interpretation of murine atrial electrophysiology.
Nonformal education as health promotion method among European youth: the example of transitional Albania
Authors: Tresa E , Burazeri G , Van den Broucke S , Qirjako G , Czabanowska K
Published: January 2021

Nonformal education methodology is promoted by the European Union as a priority. Western Balkan countries are supported in using this methodology via access Community funds (Erasmus +, previously Youth in Action). Nonformal education (proven as the most effective education method for youth) is expected to have the same impact if used in Public Health. We aimed to explore how nonformal education methodology contributes to health promotion through elaborating the example of transitional Albania. An Exploratory Sequential Mixed Methods design was used. We organized two focus groups: one with students of medical sciences and another with none medical students. We randomly selected eight participants per focus group from the Beyond Barriers association database (Contact point for Erasmus+ Programme in Albania). We used conventional content analysis to analyze qualitative data. Exploratory group interviews were conducted previously, using a questionnaire, which was piloted prior to administration. Of a population of 581 youth who participated in nonformal education activities during 2007–2013, 113 youths were interviewed. Ninety percent of interviewees declared that nonformal education activities have influenced improvement of their skills/competences or helped to acquire new ones; 53% declared that they reflected a change into personal behavior/actions/attitudes. Trainees learned through practice. They intended to retain the healthy behavior even when the activity was finished. Nonformal education activities offered equal opportunities to all youth despite their gender or field of study. Nonformal education methodology is recommended to be used in health promotion campaigns targeting young people as a very effective tool.
Aortic valve neocuspidization with in-body tissue-engineered autologous membranes: preliminary results in a long-term goat model
Authors: Kawashima Takayuki , Umeno Tadashi , Terazawa Takeshi , Wada Tomoyuki , Shuto Takashi , Nishida Haruto , Anai Hirofumi , Nakayama Yasuhide , Miyamoto Shinji
Published: January 2021

OBJECTIVES

Aortic valve neocuspidization has shown satisfactory clinical outcomes; however, autologous pericardium durability is a concern for young patients. This study applied an autologous collagenous membrane (Biosheet®), produced by in-body tissue architecture, to aortic valve neocuspidization and investigated its long-term outcome in a goat model.

METHODS

Moulds were embedded subcutaneously in 6 goats. After 2 months, Biosheets formed in the moulds. We performed aortic valve neocuspidization using a portion of the sheets with a thickness of 0.20–0.35 mm, measured by optical coherence tomography. Animals were subjected to echocardiography and histological evaluation at 6 months (n = 3) and 12 months (n = 3). As a control, the glutaraldehyde-treated autologous pericardium was used in 4 goats that were similarly evaluated at 12 months.

RESULTS

All animals survived the scheduled period. At 6 months, Biosheets maintained valve function and showed a regeneration response: fusion to the annulus, cell infiltration to the leaflets and appearance of elastic fibres at the ventricular side. After 12 months, the regenerative structure had changed little without regression, and there was negligible calcification in the 1/9 leaflets. However, all cases had one leaflet tear, resulting in moderate-to-severe aortic regurgitation. In the pericardium group, three-fourths of the animals experienced moderate-to-severe aortic regurgitation with a high rate of calcification (9/12 leaflets).

CONCLUSIONS

Biosheets may have regeneration potential and anti-calcification properties in contrast to autologous pericardium. However, in order to obtain reliable outcome, further improvements are required to strictly control and optimize its thickness, density and homogeneity.
Reverse left ventricular remodelling after aortic valve replacement for severe aortic insufficiency
Authors: Toya Teppei , Fukushima Satsuki , Shimahara Yusuke , Kasahara Shingo , Kobayashi Junjiro , Fujita Tomoyuki
Published: January 2021

OBJECTIVES

The goal of this study was to investigate the long-term outcome of aortic valve replacement (AVR) for severe aortic insufficiency with a focus on pre- and postoperative left ventricular (LV) function to explore predictive factors that influence the recovery of LV function and clinical outcome.

METHODS

A total of 478 patients who underwent AVR for pure severe aortic insufficiency were grouped according to the preoperative echocardiographical LV ejection fraction (EF): low (LO) EF <35% (n = 43), moderate EF 35–50% (n = 150) or normal EF >50% (n = 285).

RESULTS

Actuarial survival at 10 years post-AVR was 64% with a LO EF, 92% with a moderate EF and 93% with a normal EF (P = 0.016), whereas 10-year rates of freedom from major adverse cerebral and cardiovascular events were 47%, 79% and 84%, respectively (P < 0.0001). Echocardiography at 1 year post-AVR demonstrated that EF substantially improved in all groups. We noted a significant difference in survival (P = 0.0086) and in freedom from major adverse cerebral and cardiovascular events (P = 0.024) between patients with an EF ≥35% and those with an EF <35% in the LO EF group. The multivariable logistic regression model showed that predictive factors for lack of improvement in EF 1 year post-AVR in the LO EF group included plasma brain natriuretic peptide >365 pg/mL (P = 0.0022) and echocardiographic LV mass index) >193 g/m2 (P = 0.0018).

CONCLUSIONS

Long-term outcome post-AVR for severe aortic insufficiency was largely influenced by preoperative LV function. Predictive factors of failure to recover ventricular function post-AVR included EF <25%, pre-brain natriuretic peptide >365 pg/mL or LV mass index >193 g/m2.
A New Enrichment Method for Quantification of 5-Hydroxymethylfurfural by Indirect Flame Atomic Absorption Spectrometry in Honey and Jam Samples
Authors: Gürkan Ramazan
Published: January 2021

Background

Because of increasing amounts of 5-hydroxymethylfurfural (5-HMF) in processed foods and the complexity of the matrix, monitoring of trace 5-HMF requires accurate and reliable methods. Hence, an efficient sample pretreatment procedure is necessary for extraction and preconcentration of 5-HMF from the matrix.

Objective

In this study, a new and efficient sample preparation method utilizing ultrasound-assisted-cloud point extraction (UA-CPE), indirectly followed by flame atomic absorption spectrometry (FAAS), was introduced for the monitoring of trace amounts of 5-HMF in honey and jam samples.

Method

With this method, hydroxylamine was used as a derivatizing agent in the presence of Mn(II) and an anionic surfactant, SDS for extraction of 5-HMF at pH 9.0. For dispersing reagents in sample solution, low amounts of mixed surfactant, triton X-45 and SDS were mixed and fast-injected into the extraction media. A cloudy solution formed, and after reaction of 5-HMF with reagents, the cloudy solution was centrifuged. The extracted 5-HMF in the surfactant-rich phase was dissolved in acidic acetone and indirectly analyzed by FAAS.

Results

The method showed a detection limit of 1.27 μg/L in linear working range of 4–240 μg/L, good precision (2.3–6.5%), and recovery rates (93.5–97%) after preconcentration of 70-fold.

Conclusions

Within this study, an accurate and reliable method for the indirect quantification of 5-HMF in selected samples was successfully developed with a sensitivity improvement factor of 30.6.

Highlights

The figures of merit for the developed indirect method were appropriate. The applicability of the method for the analysis of 5-HMF in processed foods was excellent.
Impact of sarcopenia on post-operative outcomes following nephrectomy and tumor thrombectomy for renal cell carcinoma with inferior vena cava thrombus
Authors: Watanabe Shun , Ishihara Hiroki , Takagi Toshio , Kondo Tsunenori , Ishiyama Ryo , Fukuda Hironori , Yoshida Kazuhiko , Iizuka Junpei , Kobayashi Hirohito , Ishida Hideki , Tanabe Kazunari
Published: January 2021

Objective

Sarcopenia is associated with oncological outcomes in various types of cancer. However, the impact of sarcopenia in renal cell carcinoma with inferior vena cava thrombus remains unclear. We herein evaluated the prognostic significance of sarcopenia for renal cell carcinoma with inferior vena cava thrombus following nephrectomy and thrombectomy.

Methods

Patients who underwent nephrectomy and thrombectomy for renal cell carcinoma with inferior vena cava thrombus at our department between 2004 and 2019 were retrospectively evaluated. Their sarcopenic status, determined by sex, body mass index and skeletal muscle index, was calculated using pre-surgical radiographic imaging. We compared the post-operative cancer-specific survival and overall survival, surgical data and duration of post-operative hospitalization of sarcopenic and non-sarcopenic patients.

Results

Out of 83 patients, 54 (65%) were sarcopenic. Sarcopenic patients had significantly shorter cancer-specific survival (median: 33.3 months vs. not reached, P = 0.0323) and overall survival (32.0 months vs. not reached, P = 0.0173) than non-sarcopenic patients. Furthermore, multivariate analyses showed that sarcopenia was an independent factor for cancer-specific survival (hazard ratio: 2.76, P = 0.0212) and overall survival (hazard ratio: 2.93, P = 0.014). The incidence rate of surgical complications (any grade: 35.2% vs. 27.6%, P = 0.482; grades ≥ 3: 7.4% vs. 10.3%, P = 0.648) or duration of post-operative hospitalization (median: 11 vs. 10 days, P = 0.148) was not significantly different between sarcopenic and non-sarcopenic patients.

Conclusions

In conclusion, this study showed that sarcopenia was an independent prognostic factor for renal cell carcinoma with inferior vena cava thrombus after nephrectomy and tumor thrombectomy. Thus, sarcopenia evaluation can be utilized as an effective prognosticator of post-operative survival.
Tangeretin as an adjuvant and chemotherapeutic sensitizer against various types of cancers: a comparative overview
Authors: Alhamad Dima W , Elgendy Sara M , Al-Tel Taleb H , Omar Hany A
Published: January 2021

Objectives

Cancer is a leading cause of disabling morbidities and death worldwide. Although there are various strategies for the management of cancer, the severe adverse effects negatively impact the patient’s quality of life. In addition, the development of resistance limits the efficacy of many chemotherapeutics. Many natural agents are capable of reducing the adverse effects associated with chemotherapy and improving the therapeutic outcome. Tangeretin, a polymethoxy flavone, is one of the promising natural anticancer agents.

Key findings

Tangeretin not only targets various malignancies but also synergizes chemotherapeutic agents and reverses cancer resistance. Hence, the application of tangeretin as an adjuvant in cancer chemotherapy would be a promising strategy.

This work critically highlighted the proposed anticancer activity of tangeretin and discussed its potential combination with various chemotherapeutic agents. Additionally, it shed light on tangeretin chemical derivatives with improved pharmacokinetic and pharmacodynamic activity. Finally, this review described flavonoid biosynthetic pathways and how bioengineering can be employed to enhance the production yield of tangeretin. Thus, this work paves the way for the rational clinical utilization of tangeretin as a safe and effective adjuvant in chemotherapeutic protocols.
Pre-harvest sprouting in cereals: genetic and biochemical mechanisms
Authors: Tai Li , Wang Hong-Jin , Xu Xiao-Jing , Sun Wei-Hang , Ju Lan , Liu Wen-Ting , Li Wen-Qiang , Sun Jiaqiang , Chen Kun-Ming
Published: January 2021

With the growth of the global population and the increasing frequency of natural disasters, crop yields must be steadily increased to enhance human adaptability to risks. Pre-harvest sprouting (PHS), a term mainly used to describe the phenomenon in which grains germinate on the mother plant directly before harvest, is a serious global problem for agricultural production. After domestication, the dormancy level of cultivated crops was generally lower than that of their wild ancestors. Although the shortened dormancy period likely improved the industrial performance of cereals such as wheat, barley, rice, and maize, the excessive germination rate has caused frequent PHS in areas with higher rainfall, resulting in great economic losses. Here, we systematically review the causes of PHS and its consequences, the major indicators and methods for PHS assessment, and emphasize the biological significance of PHS in crop production. Wheat quantitative trait loci functioning in the control of PHS are also comprehensively summarized in a meta-analysis. Finally, we use Arabidopsis as a model plant to develop more complete PHS regulatory networks for wheat. The integration of this information is conducive to the development of custom-made cultivated lines suitable for different demands and regions, and is of great significance for improving crop yields and economic benefits.
Coordination between MIDASIN 1-mediated ribosome biogenesis and auxin modulates plant development
Authors: Li Ke , Zhou Ximeng , Sun Xueping , Li Guanghui , Hou Lei , Zhao Shuzhen , Zhao Chuanzhi , Ma Changle , Li Pengcheng , Wang Xingjun
Published: January 2021

Ribosomes are required for plant growth and development, and ribosome biogenesis-deficient mutants generally display auxin-related phenotypes. Although the relationship between ribosome dysfunction and auxin is known, many aspects of this subject remain to be understood. We previously reported that MIDASIN 1 (MDN1) is an essential pre-60S ribosome biogenesis factor (RBF) in Arabidopsis. In this study, we further characterized the aberrant auxin-related phenotypes of mdn1-1, a weak mutant allele of MDN1. Auxin response is disturbed in both shoots and roots of mdn1-1, as indicated by the DR5:GUS reporter. By combining transcriptome profiling analysis and reporter gene detection, we found that expression of genes involved in auxin biosynthesis, transport, and signaling is changed in mdn1-1. Furthermore, MDN1 deficiency affects the post-transcriptional regulation and protein distribution of PIN-FORMED 2 (PIN2, an auxin efflux facilitator) in mdn1-1 roots. These results indicate that MDN1 is required for maintaining the auxin system. More interestingly, MDN1 is an auxin-responsive gene, and its promoter can be targeted by multiple AUXIN RESPONSE FACTORs (ARFs), including ARF7 and ARF19, in vitro. Indeed, in arf7 arf19, the auxin sensitivity of MDN1 expression is significantly reduced. Together, our results reveal a coordination mechanism between auxin and MDN1-dependent ribosome biogenesis for regulating plant development.
The Experience of Religious Same-Sex Marriage in England and Wales: Understanding the Opportunities and Limits Created by the Marriage (Same Sex Couples) Act 2013
Authors: Falcetta Silvia , Johnson Paul , Vanderbeck Robert M
Published: January 2021

Following the enactment of the Marriage (Same Sex Couples) Act 2013, same-sex couples in England and Wales have the same opportunities as different-sex couples to marry by way of a civil ceremony. However, same-sex couples who wish to marry by way of a religious ceremony are at a significant disadvantage to different-sex couples because only a small number of religious organisations, and a tiny number of places of worship, permit same-sex marriage. Drawing on semi-structured interviews, this article provides the first analysis of the experiences of same-sex couples who have married in a certified place of worship that has been registered for same-sex marriage. We argue that these experiences are shaped in complex ways within an environment in which same-sex religious marriage is lawful but nevertheless difficult to access. The analysis examines the experiences of couples at different stages of the marriage process, including the decision to have a religious marriage, seeking a place of worship, and negotiating the form of the ceremony. We show how many couples, unable to marry within the religious traditions with which they are most familiar, are effectively ‘funnelled’ towards particular traditions about which they have little prior knowledge. The article offers a unique insight into how English marriage law sustains faith-based discrimination against same-sex couples, and how some couples are able to overcome this.
Insights in the molecular mechanisms of an azole stress adapted laboratory-generated Aspergillus fumigatus strain
Authors: Aruanno Marion , Gozel Samantha , Mouyna Isabelle , Parker Josie E , Bachmann Daniel , Flamant Patricia , Coste Alix T , Sanglard Dominique , Lamoth Frederic
Published: January 2021

Aspergillus fumigatus is the main cause of invasive aspergillosis, for which azole drugs are the first-line therapy. Emergence of pan-azole resistance among A. fumigatus is concerning and has been mainly attributed to mutations in the target gene (cyp51A). However, azole resistance may also result from other mutations (hmg1, hapE) or other adaptive mechanisms. We performed microevolution experiment exposing an A. fumigatus azole-susceptible strain (Ku80) to sub-minimal inhibitory concentration of voriconazole to analyze emergence of azole resistance. We obtained a strain with pan-azole resistance (Ku80R), which was partially reversible after drug relief, and without mutations in cyp51A, hmg1, and hapE. Transcriptomic analyses revealed overexpression of the transcription factor asg1, several ATP-binding cassette (ABC) and major facilitator superfamily transporters and genes of the ergosterol biosynthesis pathway in Ku80R. Sterol analysis showed a significant decrease of the ergosterol mass under voriconazole exposure in Ku80, but not in Ku80R. However, the proportion of the sterol compounds was similar between both strains. To further assess the role of transporters, we used the ABC transporter inhibitor milbemycine oxime (MLB). MLB inhibited transporter activity in both Ku80 and Ku80R and demonstrated some potentiating effect on azole activity. Criteria for synergism were reached for MLB and posaconazole against Ku80. Finally, deletion of asg1 revealed some role of this transcription factor in controlling drug transporter expression, but had no impact on azole susceptibility.

This work provides further insight in mechanisms of azole stress adaptation and suggests that drug transporters inhibition may represent a novel therapeutic target.

Lay Summary

A pan-azole-resistant strain was generated in vitro, in which drug transporter overexpression was a major trait. Analyses suggested a role of the transporter inhibitor milbemycin oxime in inhibiting drug transporters and potentiating azole activity.
A numerical approach to the non-uniqueness problem of cosmic ray two-fluid equations at shocks
Authors: Gupta Siddhartha , Sharma Prateek , Mignone Andrea
Published: January 2021

Cosmic rays (CRs) are frequently modelled as an additional fluid in hydrodynamic (HD) and magnetohydrodynamic (MHD) simulations of astrophysical flows. The standard CR two-fluid model is described in terms of three conservation laws (expressing conservation of mass, momentum, and total energy) and one additional equation (for the CR pressure) that cannot be cast in a satisfactory conservative form. The presence of non-conservative terms with spatial derivatives in the model equations prevents a unique weak solution behind a shock. We investigate a number of methods for the numerical solution of the two-fluid equations and find that, in the presence of shock waves, the results generally depend on the numerical details (spatial reconstruction, time stepping, the CFL number, and the adopted discretization). All methods converge to a unique result if the energy partition between the thermal and non-thermal fluids at the shock is prescribed using a subgrid prescription. This highlights the non-uniqueness problem of the two-fluid equations at shocks. From our numerical investigations, we report a robust method for which the solutions are insensitive to the numerical details even in absence of a subgrid prescription, although we recommend a subgrid closure at shocks using results from kinetic theory. The subgrid closure is crucial for a reliable post-shock solution and also its impact on large-scale flows because the shock microphysics that determines CR acceleration is not accurately captured in a fluid approximation. Critical test problems, limitations of fluid modelling, and future directions are discussed.
Overlapping but distinct: a new model for G-quadruplex biochemical specificity
Authors: Volek Martin , Kolesnikova Sofia , Svehlova Katerina , Srb Pavel , Sgallová Ráchel , Streckerová Tereza , Redondo Juan A , Veverka Václav , Curtis Edward A
Published: January 2021

G-quadruplexes are noncanonical nucleic acid structures formed by stacked guanine tetrads. They are capable of a range of functions and thought to play widespread biological roles. This diversity raises an important question: what determines the biochemical specificity of G-quadruplex structures? The answer is particularly important from the perspective of biological regulation because genomes can contain hundreds of thousands of G-quadruplexes with a range of functions. Here we analyze the specificity of each sequence in a 496-member library of variants of a reference G-quadruplex with respect to five functions. Our analysis shows that the sequence requirements of G-quadruplexes with these functions are different from one another, with some mutations altering biochemical specificity by orders of magnitude. Mutations in tetrads have larger effects than mutations in loops, and changes in specificity are correlated with changes in multimeric state. To complement our biochemical data we determined the solution structure of a monomeric G-quadruplex from the library. The stacked and accessible tetrads rationalize why monomers tend to promote a model peroxidase reaction and generate fluorescence. Our experiments support a model in which the sequence requirements of G-quadruplexes with different functions are overlapping but distinct. This has implications for biological regulation, bioinformatics, and drug design.
Experimental Constraints on the Crystallization of Silica Phases in Silicic Magmas
Authors: Martel C , Pichavant M , Di Carlo I , Champallier R , Wille G , Castro J M , Devineau K , Davydova V O , Kushnir A R L
Published: January 2021

Low-pressure silica polymorphs, e.g. quartz (Qtz), tridymite (Trd), and cristobalite (Crs), are common in silicic magmas, but the conditions of their formation are still unclear. The stability fields of these polymorphs have been determined in the SiO2, SiO2–H2O, and haplogranite systems, but these simple systems are not directly applicable to silica polymorph crystallization in natural silicic magmas. The present study compiles an experimental database of new and previously-published data documenting the crystallization of silica phases in natural silicic magmas and simple synthetic systems. Silica polymorphs are identified using Raman spectroscopy and their pressure-temperature domains of occurrence and chemical compositions are determined at pressures between 0·1 and 200 MPa, temperatures between 685 to 1200° C, and under H2O-saturated and H2O-undersaturated conditions.

 Qtz is the stable silica polymorph at pressures higher than 25–30 MPa, temperatures between ∼700 and 950° C, and occurs for a narrow range of melt SiO2 contents (∼79–81 wt %). Constraints on Qtz stability derived from simple systems are mutually compatible with, and thus applicable to natural compositions. This is consistent with Qtz compositions being close to ‘pure’ SiO2, both in experiments and nature. In volcanic systems, Qtz crystallization may occur in magmatic reservoirs and deep volcanic conduits.

 Trd did not crystallize in the experiments conducted as part of this study despite several experiments having been performed in the Trd stability field. This is consistent with results from the literature which show that Trd crystallization is kinetically inhibited in particular relative to Crs. Natural Trd have compositions deviating substantially from ‘pure’ SiO2, so that stability limits determined in simple systems should not be applied directly to natural cases.

 Crs was encountered at pressures below 20–30 MPa (or H2O contents < ∼1·5 wt %), from sub-solidus (∼800° C) to near-liquidus (up to 1040° C), and coexisting with melts having a large range of SiO2 contents (70–81 wt %). The Crs stability field is much larger in natural magmas compared to pure SiO2 systems. Crs is a metastable phase stabilized by components (Al, Na, K; about 3 wt %) present in the silicic melt. In volcanic systems, Crs crystallization may thus be restricted to subsurface manifestations such as lava domes.
Ubiquitylome analysis reveals a central role for the ubiquitin-proteasome system in plant innate immunity
Authors: Ma Xiyu , Zhang Chao , Kim Do Young , Huang Yanyan , Chatt Elizabeth , He Ping , Vierstra Richard D , Shan Libo
Published: January 2021

Protein ubiquitylation profoundly expands proteome functionality and diversifies cellular signaling processes, with recent studies providing ample evidence for its importance to plant immunity. To gain a proteome-wide appreciation of ubiquitylome dynamics during immune recognition, we employed a two-step affinity enrichment protocol based on a 6His-tagged ubiquitin (Ub) variant coupled with high sensitivity mass spectrometry to identify Arabidopsis proteins rapidly ubiquitylated upon plant perception of the microbe-associated molecular pattern (MAMP) peptide flg22. The catalog from 2-week-old seedlings treated for 30 min with flg22 contained 690 conjugates, 64 Ub footprints, and all seven types of Ub linkages, and included previously uncharacterized conjugates of immune components. In vivo ubiquitylation assays confirmed modification of several candidates upon immune elicitation, and revealed distinct modification patterns and dynamics for key immune components, including poly- and monoubiquitylation, as well as induced or reduced levels of ubiquitylation. Gene ontology and network analyses of the collection also uncovered rapid modification of the Ub-proteasome system itself, suggesting a critical auto-regulatory loop necessary for an effective MAMP-triggered immune response and subsequent disease resistance. Included targets were UBIQUITIN-CONJUGATING ENZYME 13 (UBC13) and proteasome component REGULATORY PARTICLE NON-ATPASE SUBUNIT 8b (RPN8b), whose subsequent biochemical and genetic analyses implied negative roles in immune elicitation. Collectively, our proteomic analyses further strengthened the connection between ubiquitylation and flg22-based immune signaling, identified components and pathways regulating plant immunity, and increased the database of ubiquitylated substrates in plants.