bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into the Genetic Architecture of Phenotypic Stability Traits in Winter Wheat
Authors: Lozada Dennis , Carter Arron
Published: March 2020

Examining the architecture of traits through genomics is necessary to gain a better understanding of the genetic loci affecting important traits to facilitate improvement. Genomewide association study (GWAS) and genomic selection (GS) were implemented for grain yield, heading date, and plant height to gain insights into the genetic complexity of phenotypic stability of traits in a diverse population of US Pacific Northwest winter wheat. Analysis of variance using the Additive Main Effect and Multiplicative Interaction (AMMI) approach revealed significant genotype and genotype by environment interactions. GWAS identified 12 SNP markers distributed across 10 chromosomes affecting variation for both trait and phenotypic stability, indicating potential pleiotropic effects and signifying that similar genetic loci could be associated with different aspects of stability. The lack of stable and major effect loci affecting phenotypic variation supports the complexity of stability of traits. Accuracy of GS was low to moderate, between 0.14 and 0.66, indicating that phenotypic stability is under genetic control. The moderate to high correlation between trait and trait stability suggests the potential of simultaneous selection for trait and trait stability. Our results demonstrate the complex genetic architecture of trait stability and show the potential for improving stability in winter wheat using genomic-assisted approaches.
Application of Directly Brewed Compost Extract Improves Yield and Quality in Baby Leaf Lettuce Grown Hydroponically
Authors: Giménez Almudena , Fernández Juan A. , Pascual José A. , Ros Margarita , Egea-Gilabert Catalina
Published: March 2020

The aim of this work was to study whether the application of a directly brewed compost extract (added in the nutrient solution or by microsprinkler) could be used to improve the yield and quality of baby leaf red lettuce growing in a floating system, and to control the incidence of Pythium irregulare. Its effect on the quality of fresh-cut red lettuce was also studied. For this, two experiments were carried out over two growing cycles (winter–spring and autumn). The results showed that the use of compost extract added to the nutrient solution improved baby leaf lettuce growth and quality, reducing the nitrate content and enhancing the content of potentially health-promoting compounds such as phenols and flavonoids and the antioxidant capacity. Microbial quality was maintained during storage and the compost extract had no negative effect on the microbial load of the final product. In addition, application of the compost decreased the population of P. irregulare in the water. It is concluded that the application of directly brewed compost extract is of potential use in a sustainable soilless production system for baby leaf red lettuce, since it improves the yield and quality of the product and is able to control the incidence of P. irregulare.
Farmers’ Preferences for Genetic Resources of Kersting’s Groundnut [Macrotyloma geocarpum (Harms) Maréchal and Baudet] in the Production Systems of Burkina Faso and Ghana
Authors: Coulibaly Mariam , Agossou Chaldia O.A. , Akohoué Félicien , Sawadogo Mahamadou , Achigan-Dako Enoch G.
Published: March 2020

Pulses play important roles in providing proteins and essential amino-acids, and contribute to soils’ nutrients cycling in most smallholder farming systems in Sub-Saharan Africa (SSA). These crops can be promoted to meet food and nutrition security goals in low-income countries. Here, we investigated the status of Kersting’s groundnut (Macrotyloma geocarpum, Fabaceae), a neglected pulse in West Africa. We explored its diversity, the production systems, the production constraints and farmers’ preferences in Burkina Faso and Ghana. Focus groups and semi-structured interviews were conducted in 39 villages with 86 respondents grouped in five sociolinguistic groups. Our results indicated that Macrotyloma geocarpum was produced in three cultivation systems: in the first system, farmers grew Kersting’s groundnut in fields, mostly on mounds or on ridges; in the second system, farmers grew it as field border; and in the third system, no clear tillage practice was identified. The main constraints of those farming systems included: difficulty to harvest, the lack of manpower and the damage due to high soil humidity at the reproductive stage. A total of 62 samples were collected and clustered in six landraces based on seed coat colors including cream, white mottled with black eye, white mottled with greyed orange eye, black, brown mottled, and brown. All six groups were found in the southern-Sudanian zone whereas only white mottled with black eye and black colors were found in the northern-Sudanian zone. The white mottled with black eye landrace was commonly known and widely grown by farmers. Farmers’ preferences were, however, influenced by sociolinguistic membership and the most preferred traits included high yielding, drought tolerance, and resistance against beetles. These findings offer an avenue to develop a relevant breeding research agenda for promoting Kersting’s groundnut in Burkina Faso and Ghana.
Comparison of Methods for Measuring Protein Concentration in Venom Samples
Authors: Bocian Aleksandra , Sławek Sonja , Jaromin Marcin , Hus Konrad K. , Buczkowicz Justyna , Łysiak Dawid , Petrílla Vladimir , Petrillova Monika , Legáth Jaroslav
Published: March 2020

Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.
Defatted Rice Bran Supplementation in Diets of Finishing Pigs: Effects on Physiological, Intestinal Barrier, and Oxidative Stress Parameters
Authors: Fan Lijuan , Huang Ruihua , Wu Chengwu , Cao Yang , Du Taoran , Pu Guang , Wang Huan , Zhou Wuduo , Li Pinghua , Kim Sung Woo
Published: March 2020

Rice bran is a waste product with low cost and high fiber content, giving it an added advantage over corn and soybean meal, which have to be purchased and always at a relatively higher cost. Under the background of increased attention to sustainable agriculture, it is significant to find alternative uses for this byproduct. A total of 35 finishing pigs were allotted to five dietary treatments: a control group with basal diet and four experimental diets where corn was equivalently substituted by 7%, 14%, 21%, and 28% defatted rice bran (DFRB), respectively. With increasing levels of DFRB, the neutrophil to lymphocyte ratio (NLR) linearly decreased (p < 0.05). In the jejunum, the mRNA level of nuclear factor erythroid-2 related factor-2 (Nrf2) exhibited a quadratic response (p < 0.01) with incremental levels of DFRB. In the colon, the mRNA levels of mucin 2 (MUC2), Nrf2, and NAD(P)H: quinone oxidoreductase 1 (NQO1) were upregulated (linear, p < 0.05) and heme oxygenase-1 (HO-1) was upregulated (linear, p < 0.01). Overall, using DFRB to replace corn decreased the inflammatory biomarkers of serum and showed potential function in modulating the intestinal barrier by upregulating the mRNA expression levels of MUC2 and downregulating that of Nrf2, NQO1, and HO-1 in the colon.
Ginger and Its Derivatives as Promising Alternatives to Antibiotics in Poultry Feed
Authors: Abd El-Hack Mohamed E. , Alagawany Mahmoud , Shaheen Hazem , Samak Dalia , Othman Sarah I. , Allam Ahmed A. , Taha Ayman E. , Khafaga Asmaa F. , Arif Muhammad , Osman Ali , El Sheikh Ahmed I. , Elnesr Shaaban S. , Sitohy Mahmoud
Published: March 2020

Poultry enterprises have sustained rapid development through the last three decennaries. For which reason, higher utilization of antibacterial, either as therapeutic or growth promoting agents, has been accepted. Owing to the concern of developing bacterial resistance among populations towards antibiotic generations, accumulation of antibacterial remaining’s in chicken products and elevating shopper request for outcomes without antibacterial remaining’s, looking for unconventional solutions that could exchange antibacterial without influencing productiveness or product characters. Using natural alternatives including ginger, garlic prebiotics, organic acids, plant extracts, etheric oils and immune stimulants have been applied to advance the performance, hold poultry productiveness, prevent and control the enteric pathogens and minimize the antibacterial utilization in the poultry production in recent years. The use of a single replacement or ideal assemblage of different choices besides good supervision and livestock welfare may play a basic role in maximizing benefits and preserving poultry productiveness. The object of this review was to support an outline of the recent knowledge on the use of the natural replacements (ginger and its derivatives) in poultry feed as feed additives and their effects on poultry performance, egg and meat quality, health as well as the economic efficiency.
Application of Bacillus coagulans in Animal Husbandry and Its Underlying Mechanisms
Authors: Zhou Yuanhao , Zeng Zihan , Xu Yibin , Ying Jiafu , Wang Baikui , Majeed Muhammed , Majeed Shaheen , Pande Anurag , Li Weifen
Published: March 2020

In recent decades, probiotics have attracted widespread attention and their application in healthcare and animal husbandry has been promising. Among many probiotics, Bacillus coagulans (B. coagulans) has become a key player in the field of probiotics in recent years. It has been demonstrated to be involved in regulating the balance of the intestinal microbiota, promoting metabolism and utilization of nutrients, improving immunity, and more importantly, it also has good industrial properties such as high temperature resistance, acid resistance, bile resistance, and the like. This review highlights the effects of B. coagulans in animal husbandry and its underlying mechanisms.
Population Pharmacokinetic Modeling and Pharmacodynamic Target Attainment Simulation of Piperacillin/Tazobactam for Dosing Optimization in Late Elderly Patients with Pneumonia
Authors: Ishihara Noriyuki , Nishimura Nobuhiro , Ikawa Kazuro , Karino Fumi , Miura Kiyotaka , Tamaki Hiroki , Yano Takahisa , Isobe Takeshi , Morikawa Norifumi , Naora Kohji
Published: March 2020

The aim of this study was to develop a population pharmacokinetic model for piperacillin (PIPC)/tazobactam (TAZ) in late elderly patients with pneumonia and to optimize the administration planning by applying pharmacokinetic/pharmacodynamic (PK/PD) criteria. PIPC/TAZ (total dose of 2.25 or 4.5 g) was infused intravenously three times daily to Japanese patients over 75 years old. The plasma concentrations of PIPC and TAZ were determined using high-performance liquid chromatography and modeled using the NONMEM program. PK/PD analysis with a random simulation was conducted using the final population PK model to estimate the probability of target attainment (PTA) profiles for various PIPC/TAZ-regimen–minimum-inhibitory-concentration (MIC) combinations. The PTAs for PIPC and TAZ were determined as the fraction that achieved at least 50% free time > MIC and area under the free-plasma-concentration–time curve over 24 h ≥ 96 μg h/mL, respectively. A total of 18 cases, the mean age of which was 86.5 ± 6.0 (75–101) years, were investigated. The plasma-concentration–time profiles of PIPC and TAZ were characterized by a two-compartment model. The parameter estimates for the final model, namely the total clearance, central distribution volume, peripheral distribution volume, and intercompartmental clearance, were 4.58 + 0.061 × (CLcr − 37.4) L/h, 5.39 L, 6.96 L, and 20.7 L/h for PIPC, and 5.00 + 0.059 × (CLcr − 37.4) L/h, 6.29 L, 7.73 L, and 24.0 L/h for TAZ, respectively, where CLcr is the creatinine clearance. PK/PD analysis using the final model showed that in drug-resistant strains with a MIC > 8 μg/mL, 4.5 g of PIPC/TAZ every 6 h was required, even for the patients with a CLcr of 50–60 mL/min. The population PK model developed in this study, together with MIC value, can be useful for optimizing the PIPC/TAZ dosage in the over-75-year-old patients, when they are administered PIPC/TAZ. Therefore, the findings of present study may contribute to improving the efficacy and safety of the administration of PIPC/TAZ therapy in late elderly patients with pneumonia.
Efficacy of Antioxidant Supplementation on Conventional and Advanced Sperm Function Tests in Patients with Idiopathic Male Infertility
Authors: Arafa Mohamed , Agarwal Ashok , Majzoub Ahmad , Panner Selvam Manesh Kumar , Baskaran Saradha , Henkel Ralf , Elbardisi Haitham
Published: March 2020

Antioxidants are used in the empirical treatment of infertile men. The aim of this study was to evaluate the effects of antioxidant therapy on conventional semen parameters and advanced sperm function tests in men seeking fertility treatment. A total of 148 infertile men of unknown etiology were divided into idiopathic (n = 119) and unexplained male infertility (UMI; n = 29). All participants were treated with the antioxidant supplement ‘FH PRO for Men’ for a period of three months. Compared with pretreatment results, there was a significant improvement in conventional semen parameters including sperm concentration, total and progressive motility and normal morphology, and seminal oxidation reduction potential (ORP), and sperm DNA fragmentation (SDF) in idiopathic infertile men. The changes were more prominent in idiopathic infertile men positive for ORP and SDF. UMI patients showed an improvement in progressive motility, ORP, and SDF after antioxidant treatment. Statistical analysis revealed that the efficacy of FH PRO for Men was significant in idiopathic male infertility compared with UMI. Treatment of idiopathic male infertility patients with the FH PRO for Men antioxidant regimen for three months resulted in a significant improvement in conventional semen parameters and sperm function. Therefore, FH PRO for Men offers promise for the medical treatment of idiopathic male infertility.
A Novel Computer-Aided-Diagnosis System for Breast Ultrasound Images Based on BI-RADS Categories
Authors: Chang Yi-Wei , Chen Yun-Ru , Ko Chien-Chuan , Lin Wei-Yang , Lin Keng-Pei
Published: March 2020

The breast ultrasound is not only one of major devices for breast tissue imaging, but also one of important methods in breast tumor screening. It is non-radiative, non-invasive, harmless, simple, and low cost screening. The American College of Radiology (ACR) proposed the Breast Imaging Reporting and Data System (BI-RADS) to evaluate far more breast lesion severities compared to traditional diagnoses according to five-criterion categories of masses composition described as follows: shape, orientation, margin, echo pattern, and posterior features. However, there exist some problems, such as intensity differences and different resolutions in image acquisition among different types of ultrasound imaging modalities so that clinicians cannot always identify accurately the BI-RADS categories or disease severities. To this end, this article adopted three different brands of ultrasound scanners to fetch breast images for our experimental samples. The breast lesion was detected on the original image using preprocessing, image segmentation, etc. The breast tumor’s severity was evaluated on the features of the breast lesion via our proposed classifiers according to the BI-RADS standard rather than traditional assessment on the severity; i.e., merely using benign or malignant. In this work, we mainly focused on the BI-RADS categories 2–5 after the stage of segmentation as a result of the clinical practice. Moreover, several features related to lesion severities based on the selected BI-RADS categories were introduced into three machine learning classifiers, including a Support Vector Machine (SVM), Random Forest (RF), and Convolution Neural Network (CNN) combined with feature selection to develop a multi-class assessment of breast tumor severity based on BI-RADS. Experimental results show that the proposed CAD system based on BI-RADS can obtain the identification accuracies with SVM, RF, and CNN reaching 80.00%, 77.78%, and 85.42%, respectively. We also validated the performance and adaptability of the classification using different ultrasound scanners. Results also indicate that the evaluations of F-score based on CNN can obtain measures higher than 75% (i.e., prominent adaptability) when samples were tested on various BI-RADS categories.
Magnetic Nanowires
Authors: Piraux Luc
Published: March 2020

Magnetic nanowires are attractive materials because of their morphology-dependent remarkable properties suitable for various advanced technologies in sensing, data storage, spintronics, biomedicine and microwave devices, etc. The recent advances in synthetic strategies and approaches for the fabrication of complex structures, such as parallel arrays and 3D networks of one-dimensional nanostructures, including nanowires, nanotubes, and multilayers, are presented. The simple template-assisted electrodeposition method enables the fabrication of different nanowire-based architectures with excellent control over geometrical features, morphology and chemical composition, leading to tunable magnetic, magneto-transport and thermoelectric properties. This review article summarizing the work carried out at UCLouvain focuses on the magnetic and spin-dependent transport properties linked to the material and geometrical characteristics.
A Multi-Terminal HVdc Grid Topology Proposal for Offshore Wind Farms
Authors: Raza Ali , Younis Muhammad , Liu Yuchao , Altalbe Ali , Rouzbehi Kumars , Abbas Ghulam
Published: March 2020

Although various topologies of multi-terminal high voltage direct current (MT-HVdc) transmission systems are available in the literature, most of them are prone to loss of flexibility, reliability, stability, and redundancy in the events of grid contingencies. In this research, two new wind farms and substation ring topology (2WF-SSRT) are designed and proposed to address the aforementioned shortcomings. The objective of this paper is to investigate MT-HVdc grid topologies for integrating large offshore wind farms with an emphasis on power loss in the event of a dc grid fault or mainland alternating current (ac)grid abnormality. Standards and control of voltage source converter (VSC) based MT-HVdc grids are defined and discussed. High voltage dc switch-gear and dc circuit topologies are appraised based on the necessity of dc cables, HVdc circuit breakers, and extra offshore platforms. In this paper, the proposed topology is analyzed and compared with the formers for number and ratings of offshore substations, dc breakers, ultra-fast mechanical actuators, dc circuits, cost, flexibility, utilization, and redundancy of HVdc links. Coordinated operation of various topologies is assessed and compared with respect to the designed control scheme via a developed EMTDC/PSCAD simulation platform considering three fault scenarios: dc fault on transmission link connecting the wind farm to mainland power converters, dc fault within substation ring of VSC-HVdc stations, and ultimate disconnection of grid side VSC station. Results show that 2WF-SSRT is a promising topology for future MT-HVdc grids.
Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease
Authors: Zannella Carla , Shinde Surbhi , Vitiello Mariateresa , Falanga Annarita , Galdiero Emilia , Fahmi Amir , Santella Biagio , Nucci Ludovica , Gasparro Roberta , Galdiero Marilena , Boccellino Mariarosaria , Franci Gianluigi , Di Domenico Marina
Published: March 2020

(1) Background: In dentistry, silver nanoparticles (AgNPs) have progressively earned great interest as antimicrobial drugs and are widely used in several biomedical fields. Recent progress in the analysis of complex bacterial communities has demonstrated the richness of the oral microbiota and the presence of numerous previously unexplained strains. Several efforts have been dedicated to the investigation of antimicrobial peptides (AMPs). Those peptides are a widespread group of small peptides against invading microbes. We report the production of a hybrid molecule composed of AgNPs and indolicidin, a well-known antibacterial peptide. (2) Methods: Spectroscopy and microscopy were used to analyze the optical features and to determine the size of the generated AgNPs. The AgNP antibacterial activity was evaluated versus oral Gram-positive and Gram-negative bacteria. (3) Results: The coated nanoparticles’ antibacterial activity strongly inhibited the growth of microorganisms, with very low minimum inhibitory concentration (MIC) values in the range of 5–12.5 μg/mL. We hypothesize that this effect depended on the specific characteristics of the metal surface coated with indolicidin. The second result was that the coated nanoparticles observed cellular toxicity, was lower with respect to the toxicity of peptide and the naked AgNPs when used individually. (4) New investigations regarding antimicrobial effect of AgNPs coated with AMPs in oral infections are an urgent task.
RBC Storage Lesion Studies in Humans and Experimental Models of Shock
Authors: Applefeld Willard N. , Wang Jeffrey , Solomon Steven B. , Sun Junfeng , Klein Harvey G. , Natanson Charles
Published: March 2020

The finding of toxicity in a meta-analysis of observational clinical studies of transfused longer stored red blood cells (RBC) and ethical issues surrounding aging blood for human studies prompted us to develop an experimental model of RBC transfusion. Transfusing older RBCs during canine pneumonia increased mortality rates. Toxicity was associated with in vivo hemolysis with release of cell-free hemoglobin (CFH) and iron. CFH can scavenge nitric oxide, causing vasoconstriction and endothelial injury. Iron, an essential bacterial nutrient, can worsen infections. This toxicity was seen at commonly transfused blood volumes (2 units) and was altered by the severity of pneumonia. Washing longer-stored RBCs mitigated these detrimental effects, but washing fresh RBCs actually increased them. In contrast to septic shock, transfused longer stored RBCs proved beneficial in hemorrhagic shock by decreasing reperfusion injury. Intravenous iron was equivalent in toxicity to transfusion of longer stored RBCs and both should be avoided during infection. Storage of longer-stored RBCs at 2 °C instead of higher standard temperatures (4–6 °C) minimized the release of CFH and iron. Haptoglobin, a plasma protein that binds CFH and increases its clearance, minimizes the toxic effects of longer-stored RBCs during infection and is a biologically plausible novel approach to treat septic shock.
Production of Sorbitol via Catalytic Transfer Hydrogenation of Glucose
Authors: García Beatriz , Moreno Jovita , Morales Gabriel , Melero Juan A. , Iglesias Jose
Published: March 2020

Sorbitol production from glucose was studied through catalytic transfer hydrogenation (CTH) over Raney nickel catalysts in alcohol media, used as solvents and hydrogen donors. It was found that alcohol sugars, sorbitol and mannitol, can be derived from two hydrogen transfer pathways, one produced involving the sacrificing alcohol as a hydrogen donor, and a second one involving glucose disproportionation. Comparison between short-chain alcohols evidenced that ethanol was able to reduce glucose in the presence of Raney nickel under neutral conditions. Side reactions include fructose and mannose production via glucose isomerization, which occur even in the absence of the catalyst. Blank reaction tests allowed evaluating the extension of the isomerization pathway. The influence of several operation parameters, like the temperature or the catalyst loading, as well as the use of metal promoters (Mo and Fe-Cr) over Raney nickel, was examined. This strategy opens new possibilities for the sustainable production of sugar alcohols.
Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey
Authors: M. Afonso Alexandra , Gonçalves Joana , Luís Ângelo , Gallardo Eugenia , Duarte Ana Paula
Published: March 2020

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.
An Optimized Covering Spheroids by Spheres
Authors: Pankratov Alexander , Romanova Tatiana , Litvinchev Igor , Marmolejo-Saucedo Jose Antonio
Published: March 2020

Covering spheroids (ellipsoids of revolution) by different spheres is studied. The research is motivated by packing non-spherical particles arising in natural sciences, e.g., in powder technologies. The concept of an ε -cover is introduced as an outer multi-spherical approximation of the spheroid with the proximity ε . A fast heuristic algorithm is proposed to construct an optimized ε -cover giving a reasonable balance between the value of the proximity parameter ε and the number of spheres used. Computational results are provided to demonstrate the efficiency of the approach.
Three-Dimensional CAD in Skull Reconstruction: A Narrative Review with Focus on Cranioplasty and Its Potential Relevance to Brain Sciences
Authors: Kung Woon-Man , Tzeng I-Shiang , Lin Muh-Shi
Published: March 2020

In patients suffering from severe traumatic brain injury and massive stroke (hemorrhagic or ischemic), decompressive craniectomy (DC) is a surgical strategy used to reduce intracranial pressure, and to prevent brainstem compromise from subsequent brain edema. In surviving patients, cranioplasty surgery helps to protect brain tissue, and correct the external deformity. The aesthetic outcome of cranioplasty using an asymmetrical implant can negatively influence patients physically and mentally, especially young patients. Advancements in the development of biomaterials have now made three-dimensional (3-D) computer-assisted design/manufacturing (CAD/CAM)-fabricated implants an optimal choice for the repair of skull defects following DC. Here, we summarize the various materials for cranioplasty, including xenogeneic, autogenous, and alloplastic grafts. The processing procedures of the CAD/CAM technique are briefly outlined, and reflected our experiences to reconstruct skull CAD models using commercial software, published previously, to assess aesthetic outcomes of regular 3-D CAD models without contouring elevation or depression. The establishment of a 3-D CAD model ensures a possibility for better aesthetic outcomes of CAM-derived alloplastic implants. Finally, clinical consideration of the CAD algorithms for adjusting contours and their potential application in prospective healthcare are briefly outlined.
Deadbeat Predictive Current Control for Circulating Currents Reduction in a Modular Multilevel Converter Based Rail Power Conditioner
Authors: Tanta Mohamed , Pinto J. G. , Monteiro Vitor , Martins Antonio P. , Carvalho Adriano S. , Afonso Joao L.
Published: March 2020

This paper presents a deadbeat predictive current control methodology to reduce the circulating currents in a modular multilevel converter (MMC) when it operates as a rail power conditioner (RPC) in a conventional railway system-based V/V connection. For this purpose, a half-bridge MMC based on half-bridge submodules, operating as an RPC is explained, and the total system is denominated as a simplified rail power conditioner (SRPC). The SRPC in this study is used to compensate harmonics, reactive power, and the negative sequence component of currents. This paper explains the SRPC system architecture, the key control algorithms, and the deadbeat predictive current control methodology. Mathematical analysis, based on the MMC equivalent circuit, is described and the reference equations are presented. Moreover, simulation results of the deadbeat predictive current control methodology are compared with the results of the conventional proportional-integral (PI) controller. This comparison is to verify the effectiveness of the proposed control strategy. Simulation results of the SRPC show reduced circulating currents in the MMC phases when using the predictive control approach, besides accomplishing power quality improvement at the three-phase power grid side.
Mindfulness for Preventing Psychosocial Risks in the Workplace: A Systematic Review and Meta-Analysis
Authors: Pérez-Fuentes María del Carmen , Molero Jurado María del Mar , Mercader Rubio Isabel , Soriano Sánchez José Gabriel , Gázquez Linares José Jesús
Published: March 2020

Organizations today are implementing psychological interventions to promote the job performance and wellbeing of their employees. Mindfulness-based interventions (MBIs) can contribute to providing workers with competencies and skills and develop their strengths. MBIs are therefore becoming more and more present in the workplace, sometimes using online intervention programs, which offer a promising direction in prevention and intervention for health. The objective of this study is to analyze the efficacy of MBIs on psychological variables in the workplace. For this purpose, a search for scientific articles published from 2009 to 2019 was made in the Psicodoc, PsycINFO, and Web of Science databases, where a total of 468 articles were found. After filtering with preestablished inclusion criteria, 24 studies were included in the systematic review and meta-analysis. The results of the meta-analysis suggest that intervention in mindfulness positively influences psychological variables related to employee health and wellbeing. However, it is recommended to continue performing new studies to confirm this finding.
Sliding Mode Switch Control of Adjustable Hydro-Pneumatic Suspension based on Parallel Adaptive Clonal Selection Algorithm
Authors: Zhou Chen , Liu Xinhui , Xu Feixiang , Chen Wei
Published: March 2020

The hydro-pneumatic suspension, as a widely used suspension for heavy vehicles, has been taken seriously by researchers for a long time because it is crucial in terms of handling stability, riding comfort, and driving safety of these vehicles. Most previous studies only discussed the control of ride comfort or vehicle handling stability of the suspension system separately. This article proposes a dynamic switch control strategy which can switch between ride comfort and handling stability controllers under different road surfaces and driving conditions. The load transfer ratio (LTR) is selected as the switch performance index, and it is calculated through a six-degrees-of-freedom (6-DOF) model. The ride comfort and handling stability controller of the hydro-pneumatic suspension are designed based on the sliding mode control theory. The objective functions of parameters optimization of the sliding mode controller (SMC) are obtained by means of analytic hierarchy process (AHP), and then the controller’s parameters are optimized by the parallel adaptive clonal selection algorithm (PACSA). The simulation results based on MATLAB/Simulink show that: (1) the PACSA performs better than a genetic algorithm in terms of the parameters optimization of the SMC; (2) the proposed switch control strategy can simultaneously improve the ride comfort and handling stability under several typical steering maneuvers and various road profiles compared with the conventional SMC-controlled suspension.
Solving a System of Differential Equations Containing a Diffusion Equation with Nonlinear Terms on the Example of Laser Heating in Silicon
Authors: Lipp Vladimir , Rethfeld Baerbel , Garcia Martin , Ivanov Dmitry
Published: March 2020

We present a finite-difference integration algorithm for solution of a system of differential equations containing a diffusion equation with nonlinear terms. The approach is based on Crank–Nicolson method with predictor–corrector algorithm and provides high stability and precision. Using a specific example of short-pulse laser interaction with semiconductors, we give a detailed description of the method and apply it to the solution of the corresponding system of differential equations, one of which is a nonlinear diffusion equation. The calculated dynamics of the energy density and the number density of photoexcited free carriers upon the absorption of laser energy are presented for the irradiated thin silicon film. The energy conservation within 0.2 % has been achieved for the time step 10 8 times larger than that in case of the explicit scheme, for the chosen numerical setup. The implemented Fortran source code is available in the Supplementary Materials. We also present a few examples of successful application of the method demonstrating its benefits for the theoretical studies of laser–matter interaction problems. Finally, possible extension to 2 and 3 dimensions is discussed.
Comprehensive Document Summarization with Refined Self-Matching Mechanism
Authors: Zeng Biqing , Xu Ruyang , Yang Heng , Gan Zibang , Zhou Wu
Published: March 2020

Under the constraint of memory capacity of the neural network and the document length, it is difficult to generate summaries with adequate salient information. In this work, the self-matching mechanism is incorporated into the extractive summarization system at the encoder side, which allows the encoder to optimize the encoding information at the global level and effectively improves the memory capacity of conventional LSTM. Inspired by human coarse-to-fine understanding mode, localness is modeled by Gaussian bias to improve contextualization for each sentence, and merged into the self-matching energy. The refined self-matching mechanism not only establishes global document attention but perceives association with neighboring signals. At the decoder side, the pointer network is utilized to perform a two-hop attention on context and extraction state. Evaluations on the CNN/Daily Mail dataset verify that the proposed model outperforms the strong baseline models and statistical significantly.
Retrieval and Analysis of the Strongest Mixed Layer in the Troposphere
Authors: Sheng Zheng , Zhou Lesong , He Yang
Published: March 2020

In this article, Thorpe analysis, which often retrieves the characteristics of mixing in the free atmosphere from balloon sounding data, is applied to the data of the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC). We find that the COSMIC data can well retrieve the strongest mixed layer in the troposphere (SMLT) altitude, and can reveal the basic variation trend of the SMLT thickness and Thorpe scale L T . We use COSMIC data to reveal the global spatial and temporal distribution of the SMLT from 2007 to 2015 and analyze the fluctuation period of the SMLT altitude with Hilbert–Huang transform (HHT), we find that the variation of the SMLT altitude is influenced by the dual effects of terrain and solar radiation.
Evidence of Ozone-Induced Visible Foliar Injury in Hong Kong Using Phaseolus Vulgaris as a Bioindicator
Authors: Leung Felix , Pang Jacky Y. S. , Tai Amos P. K. , Lam Timothy , Tao Donald K. C. , Sharps Katrina
Published: March 2020

(1) Background: Hong Kong is one of the most densely populated cities in the world, with millions of people exposed to severe air pollution. Surface ozone, mostly produced photochemically from anthropogenic precursor gases, is harmful to both humans and vegetation. The phytotoxicity of ozone has been shown to damage plant photosynthesis, induce early leaf death, and retard growth. (2) Methods: We use genotypes of bush bean Phaseolus vulgaris with various degrees of sensitivity to ozone to investigate the impacts of ambient ozone on the morphology and development of the beans. We use ozone-induced foliar injury index and measure the flowering and fruit production to quantify the ozone stress on the plants. (3) Results: We expected that the ozone-sensitive genotype would suffer from a reduction of yield. Results, however, show that the ozone-sensitive genotype suffers higher ozone-induced foliar damage as expected but produces more pods and beans and heavier beans than the ozone-resistant genotype. (4) Conclusions: It is postulated that the high ozone sensitivity of the sensitive genotype causes stress-induced flowering, and therefore results in higher bean yield. A higher than ambient concentration of ozone is needed to negatively impact the yield production of the ozone-sensitive genotype. Meanwhile, ozone-induced foliar damage shows a graduated scale of damage pattern that can be useful for indicating ozone levels. This study demonstrates the usefulness of bioindicators to monitor the phytotoxic effects of ozone pollution in a subtropical city such as Hong Kong.
System-Wide Analysis of Protein Acetylation and Ubiquitination Reveals a Diversified Regulation in Human Cancer Cells
Authors: Kozuka-Hata Hiroko , Kitamura Aya , Hiroki Tomoko , Aizawa Aiko , Tsumoto Kouhei , Inoue Jun-ichiro , Oyama Masaaki
Published: March 2020

Post-translational modifications are known to be widely involved in the regulation of various biological processes, through the extensive diversification of each protein function at the cellular network level. In order to unveil the system-wide function of the protein lysine modification in cancer cell signaling, we performed global acetylation and ubiquitination proteome analyses of human cancer cells, based on high-resolution nanoflow liquid chromatography–tandem mass spectrometry, in combination with the efficient biochemical enrichment of target modified peptides. Our large-scale proteomic analysis enabled us to identify more than 5000 kinds of ubiquitinated sites and 1600 kinds of acetylated sites, from representative human cancer cell lines, leading to the identification of approximately 900 novel lysine modification sites in total. Very interestingly, 236 lysine residues derived from 141 proteins were found to be modified with both ubiquitination and acetylation. As a consequence of the subsequent motif extraction analyses, glutamic acid (E) was found to be highly enriched at the position (−1) for the lysine acetylation sites, whereas the same amino acid was relatively dispersed along the neighboring residues of the lysine ubiquitination sites. Our pathway analysis also indicated that the protein translational control pathways, such as the eukaryotic initiation factor 2 (EIF2) and the ubiquitin signaling pathways, were highly enriched in both of the acetylation and ubiquitination proteome data at the network level. This report provides the first integrative description of the protein acetylation and ubiquitination-oriented systematic regulation in human cancer cells.
Adsorption of Fibrinogen on Silica Surfaces—The Effect of Attached Nanoparticles
Authors: Hyltegren Kristin , Hulander Mats , Andersson Martin , Skepö Marie
Published: March 2020

When a biomaterial is inserted into the body, proteins rapidly adsorb onto its surface, creating a conditioning protein film that functions as a link between the implant and adhering cells. Depending on the nano-roughness of the surface, proteins will adsorb in different amounts, with different conformations and orientations, possibly affecting the subsequent attachment of cells to the surface. Thus, modifications of the surface nanotopography of an implant may prevent biomaterial-associated infections. Fibrinogen is of particular importance since it contains adhesion epitopes that are recognized by both eukaryotic and prokaryotic cells, and can therefore influence the adhesion of bacteria. The aim of this study was to model adsorption of fibrinogen to smooth or nanostructured silica surfaces in an attempt to further understand how surface nanotopography may affect the orientation of the adsorbed fibrinogen molecule. We used a coarse-grained model, where the main body of fibrinogen (visible in the crystal structure) was modeled as rigid and the flexible α C-chains (not visible in the crystal structure) were modeled as completely disordered. We found that the elongated fibrinogen molecule preferably adsorbs in such a way that it protrudes further into solution on a nanostructured surface compared to a flat one. This implicates that the orientation on the flat surface increases its bio-availability.
Probabilistic Life-Cycle Assessment of Service Life Extension on Renovated Buildings under Seismic Hazard
Authors: Di Bari Roberta , Belleri Andrea , Marini Alessandra , Horn Rafael , Gantner Johannes
Published: March 2020

Existing buildings can reach a performance enhancement and extend their nominal service life through renovation measures such as seismic rehabilitation. In particular, when buildings have almost exhausted their service life, seeking an optimal solution should consider whether costs and environmental effects are worthwhile, or new construction is preferred. In this paper, a methodology to consider seismic hazard into probabilistic approaches for life-cycle analyses is presented considering the possibility of structural enhancement over an extended building lifespan. A life-cycle-based decision support tool for building renovation measures is developed and applied to a selected case study. Unlike standard “static” analyses, which in this work show shortcomings by underestimating impacts of vulnerable buildings, such an approach brings out environmental and economic advantages of retrofit measures designed to improve the structural performance.
EGFR Protein Expression in KRAS Wild-Type Metastatic Colorectal Cancer Is Another Negative Predictive Factor of the Cetuximab Therapy
Authors: Uhlyarik Andrea , Piurko Violetta , Papai Zsuzsanna , Raso Erzsebet , Lahm Erika , Kiss Edina , Sikter Marta , Vachaja Jozsef , Kenessey Istvan , Timar Jozsef
Published: March 2020

The selection of colorectal cancer patients for anti-epidermal growth factor receptor (EGFR) antibody therapy is based on the determination of their RAS mutation status—a strongly negative predictive factor—since the protein target, EGFR, is not a reliable predictor of therapeutic response. In this study, we revisited the EGFR protein issue using a cohort of 90 patients with KRAS exon2 wild-type colorectal cancer who have been treated with cetuximab therapy. Twenty-nine of these patients had metastatic tissue available for analysis. The level of EGFR protein expression in the patients was determined by immunohistochemistry and evaluated by H-score (HS) methodology. Progression-free survival (PFS) and overall survival (OS) of the patients were determined according to the EGFR-HS ranges of both the primary and metastatic tissues using Kaplan–Meyer statistics. In the case of primary tumors, EGFR scores lower than HS = 200 were associated with significantly longer OS. In the case of metastatic tissues, all levels lower than the EGFR-HS range chosen were associated with significantly longer OS. These results are explained by the fact that metastatic tissues rarely maintained the expression levels of the primary tumors. On the other hand, high EGFR expression levels in either primary tumors or metastatic tissues were associated with multiple metastatic disease. This suggests a negative prognostic role of EGFR expression. However, in a multivariate analysis, one-sidedness remained a strong independent predictive factor of survival. Previous studies demonstrated that the EGFR expression level depends on sidedness. Therefore, a subgroup analysis of the left- and right-sided cases was performed on both primary and metastatic tissues. In the case of metastic tissues, an analysis confirmed a better OS in low EGFR protein-expressing cases than in high EGFR protein-expressing cases. Collectively, these data suggest that EGFR protein expression is another negative predictive factor of the efficacy of cetuximab therapy of KRAS exon2 wild-type colorectal cancer.
Tumor Suppressors in Chronic Lymphocytic Leukemia: From Lost Partners to Active Targets
Authors: Andreani Giacomo , Carrà Giovanna , Lingua Marcello Francesco , Maffeo Beatrice , Brancaccio Mara , Taulli Riccardo , Morotti Alessandro
Published: March 2020

Tumor suppressors play an important role in cancer pathogenesis and in the modulation of resistance to treatments. Loss of function of the proteins encoded by tumor suppressors, through genomic inactivation of the gene, disable all the controls that balance growth, survival, and apoptosis, promoting cancer transformation. Parallel to genetic impairments, tumor suppressor products may also be functionally inactivated in the absence of mutations/deletions upon post-transcriptional and post-translational modifications. Because restoring tumor suppressor functions remains the most effective and selective approach to induce apoptosis in cancer, the dissection of mechanisms of tumor suppressor inactivation is advisable in order to further augment targeted strategies. This review will summarize the role of tumor suppressors in chronic lymphocytic leukemia and attempt to describe how tumor suppressors can represent new hopes in our arsenal against chronic lymphocytic leukemia (CLL).