bluuebunny/crossref_metadata_2025 huggingface dataset:
Ticagrelor Exerts Immune-Modulatory Effect by Attenuating Neutrophil Extracellular Traps
Authors: Mitsios Alexandros , Chrysanthopoulou Akrivi , Arampatzioglou Athanasios , Angelidou Iliana , Vidali Veroniki , Ritis Konstantinos , Skendros Panagiotis , Stakos Dimitrios
Published: May 2020

Neutrophils through the release of neutrophil extracellular traps (NETs) containing active tissue factor (TF) are key components of thrombo-inflammation. Platelets-neutrophils interplay in ST elevation myocardial infarction (STEMI) promotes NET formation via inorganic polyphosphates (polyP) released by thrombin-activated platelets. NETs, however, are also induced by biomaterials in a platelet-independent manner. Considering the possible pleiotropic effects of Ticagrelor beyond platelet inhibition and the clinical need for novel antithrombotic strategies targeting inflammation, we investigated the effects of Ticagrelor on polyP and stent-induced NETs in STEMI. Neutrophils from healthy individuals and patients receiving Ticagrelor were stimulated with polyP or drug-eluting stents (DES) to produce NETs. To induce TF expression, neutrophils were further incubated with plasma obtained from the infarct-related artery (IRA) of STEMI patients. The effects of Ticagrelor on NETs and TF loading were assessed using fluorescence microscopy, flow cytometry, myeloperoxidase(MPO)/DNA complex ELISA, and a Western blot. Ticagrelor interrupts platelet–neutrophil interaction by attenuating NETs induced by polyP. However, Ticagrelor does not affect polyP secretion from thrombin-activated platelets. Similarly, the intracellular production of TF in neutrophils triggered by IRA plasma is not hindered by Ticagrelor. Furthermore, DES induce NETs and synchronous stimulation with IRA plasma leads to the formation of thrombogenic TF-bearing NETs. Ticagrelor inhibits stent-induced NET release. These findings suggest a novel immune-modulatory effect of Ticagrelor when it attenuates the formation of thrombogenic NETs.
Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis
Authors: Buyandelger Undral , Walker Douglas G. , Yanagisawa Daijiro , Morimura Toshifumi , Tooyama Ikuo
Published: May 2020

Aberrant angiogenesis is a pathological feature of a number of diseases and arises from the uncoordinated expression of angiogenic factors as response to different cellular stresses. Age-related macular degeneration (AMD), a leading cause of vision loss, can result from pathological angiogenesis. As a mutation in the mitochondrial ferritin (FTMT) gene has been associated with AMD, its possible role in modulating angiogenic factors and angiogenesis was investigated. FTMT is an iron-sequestering protein primarily expressed in metabolically active cells and tissues with high oxygen demand, including retina. In this study, we utilized the human retinal pigment epithelial cell line ARPE-19, both as undifferentiated and differentiated cells. The effects of proinflammatory cytokines, FTMT knockdown, and transient and stable overexpression of FTMT were investigated on expression of pro-angiogenic vascular endothelial growth factor (VEGF) and anti-angiogenic pigment epithelial-derived factor (PEDF). Proinflammatory cytokines induced FTMT and VEGF expression, while NF-κB inhibition significantly reduced FTMT expression. VEGF protein and mRNA expression were significantly increased in FTMT-silenced ARPE-19 cells. Using an in vitro angiogenesis assay with endothelial cells, we showed that conditioned media from FTMT-overexpressing cells had significant antiangiogenic effects. Collectively, our findings indicate that increased levels of FTMT inhibit angiogenesis, possibly by reducing levels of VEGF and increasing PEDF expression. The cellular models developed can be used to investigate if increased FTMT may be protective in angiogenic diseases, such as AMD.
Melatonin from an Antioxidant to a Classic Hormone or a Tissue Factor: Experimental and Clinical Aspects 2019
Authors: Mayo Juan C. , Sainz Rosa M.
Published: May 2020

During the last 25 years we have accomplished great advances in melatonin research, regarding antioxidant or anti-inflammatory functions, oncostatic actions, glucose metabolism regulation or plant physiology, among others. Of course, we should not forget the classical, circadian-related functions of the indole, which has recently brought up new and important findings. All together these new discoveries will likely lead the way in the next decade in terms of melatonin research. This special issue collects some of these new advances focused on different aspects of the indole
SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner
Authors: Moroni Lorenzo , Barbaro Fulvio , Caiment Florian , Coleman Orla , Costagliola Sabine , Di Conza Giusy , Elviri Lisa , Giselbrecht Stefan , Krause Christian , Mota Carlos , Nazzari Marta , Pennington Stephen R. , Ringwald Annette , Sandri Monica , Thomas Simon , Waddington James , Toni Roberto
Published: May 2020

Endocrine disruptors (EDs) are chemicals that contribute to health problems by interfering with the physiological production and target effects of hormones, with proven impacts on a number of endocrine systems including the thyroid gland. Exposure to EDs has also been associated with impairment of the reproductive system and incidence in occurrence of obesity, type 2 diabetes, and cardiovascular diseases during ageing. SCREENED aims at developing in vitro assays based on rodent and human thyroid cells organized in three different three-dimensional (3D) constructs. Due to different levels of anatomical complexity, each of these constructs has the potential to increasingly mimic the structure and function of the native thyroid gland, ultimately achieving relevant features of its 3D organization including: (1) a 3D organoid based on stem cell-derived thyrocytes, (2) a 3D organoid based on a decellularized thyroid lobe stromal matrix repopulated with stem cell-derived thyrocytes, and (3) a bioprinted organoid based on stem cell-derived thyrocytes able to mimic the spatial and geometrical features of a native thyroid gland. These 3D constructs will be hosted in a modular microbioreactor equipped with innovative sensing technology and enabling precise control of cell culture conditions. New superparamagnetic biocompatible and biomimetic particles will be used to produce “magnetic cells” to support precise spatiotemporal homing of the cells in the 3D decellularized and bioprinted constructs. Finally, these 3D constructs will be used to screen the effect of EDs on the thyroid function in a unique biological sex-specific manner. Their performance will be assessed individually, in comparison with each other, and against in vivo studies. The resulting 3D assays are expected to yield responses to low doses of different EDs, with sensitivity and specificity higher than that of classical 2D in vitro assays and animal models. Supporting the “Adverse Outcome Pathway” concept, proteogenomic analysis and biological computational modelling of the underlying mode of action of the tested EDs will be pursued to gain a mechanistic understanding of the chain of events from exposure to adverse toxic effects on thyroid function. For future uptake, SCREENED will engage discussion with relevant stakeholder groups, including regulatory bodies and industry, to ensure that the assays will fit with purposes of ED safety assessment. In this project review, we will briefly discuss the current state of the art in cellular assays of EDs and how our project aims at further advancing the field of cellular assays for EDs interfering with the thyroid gland.
Interstitial Leydig Cell Tumorigenesis—Leptin and Adiponectin Signaling in Relation to Aromatase Expression in the Human Testis
Authors: Duliban Michal , Gorowska-Wojtowicz Ewelina , Tworzydlo Waclaw , Rak Agnieszka , Brzoskwinia Malgorzata , Krakowska Izabella , Wolski Jan K. , Kotula-Balak Malgorzata , Płachno Bartosz J. , Bilinska Barbara
Published: May 2020

Although epidemiological studies from the last years report an increase in the incidences of Leydig cell tumors (previously thought to be a rare disease), the biochemical characteristics of that tumor important for understanding its etiology, diagnosis, and therapy still remains not completely characterized. Our prior studies reported G-protein coupled estrogen receptor signaling and estrogen level disturbances in Leydig cell tumors. In addition, we found that expressions of multi-level-acting lipid balance- and steroidogenesis–controlling proteins including peroxisome proliferator-activated receptor are altered in this tumor. In order to get deeper into the other molecular mechanisms that regulate lipid homeostasis in the Leydig cell tumor, here we investigate the presence and expression of newly-described hormones responsible for lipid homeostasis balancing (leptin and adiponectin), together with expression of estrogen synthase (aromatase). Samples of Leydig cell tumors (n = 20) were obtained from patients (31–45 years old) and used for light and transmission electron microscopic, western blotting, and immunohistochemical analyses. In addition, body mass index (BMI) was calculated. In tumor mass, abundant lipid accumulation in Leydig cells and various alterations of Leydig cell shape, as well as the presence of adipocyte-like cells, were observed. Marked lipid content and various lipid droplet size, especially in obese patients, may indicate alterations in lipid homeostasis, lipid processing, and steroidogenic organelle function in response to interstitial tissue pathological changes. We revealed significantly increased expression of leptin, adiponectin and their receptors, as well as aromatase in Leydig cell tumors in comparison to control. The majority of patients (n = 13) were overweight as indicated by their BMI. Moreover, a significant increase in expression of phospholipase C (PLC), and kinases Raf, ERK which are part of adipokine transductional pathways, was demonstrated. These data expand our previous findings suggesting that in human Leydig cell tumors, estrogen level and signaling, together with lipid status, are related to each other. Increased BMI may contribute to certain biochemical characteristics and function of the Leydig cell in infertile patients with a tumor. In addition, altered adipokine-estrogen microenvironment can have an effect on proliferation, growth, and metastasis of tumor cells. We report here various targets (receptors, enzymes, hormones) controlling lipid balance and estrogen action in Leydig cell tumors indicating their possible usefulness for diagnostics and therapy.
Dry Sliding Friction of Tool Steels and Their Comparison of Wear in Contact with ZrO2 and X46Cr13
Authors: Krbata Michal , Eckert Maros , Bartosova Lenka , Barenyi Igor , Majerik Jozef , Mikuš Pavol , Rendkova Petra
Published: May 2020

Tool steels are used in stamping, shearing processes, and as cutting tools due to their good mechanical properties. During their working cycle, steels are subject to aggressive conditions such as heat stress, fatigue, and wear. In this paper, three tool steels, namely X153CrMoV12, X37CrMoV5-1, and X45NiCrMo4 were selected against two types of bearing balls, ZrO2 and X46Cr1. All measurements were performed on a UMT TriboLab universal tribometric instrument under dry conditions. The main objective of the experiment was to analyze and compare tool steel wear in contact with two kinds of bearing balls with a diameter of 4.76 mm. This evaluation is focused on the hardness, surface roughness, and microstructure of all samples and on the impact of the input parameters on the resulting wear. All three types of tool steels were measured in the basic annealed state and, subsequently, in the state after hardening and tempering. Experimental results show that tool steels, belonging to high strength steels, can successfully represent wear resistant steels. The content of carbide elements, their size, and shape in the microstructure play an important role in the friction process and subsequent wear. Three types of loads were used and compared in the experiments 30, 60, and 90 N. Increasing the load results in significant degradation of the material on the sample surface. Lastly, the impact of hardness and roughness of materials on wear has also been proven. If abrasive wear occurs in the friction process, there is a greater degree of wear than that of adhesive wear. This is due to less abrasive particles, which behave like a cutting wedge and are subject to subsequent deformation strengthening due to the load increase, which adversely affects the further friction process. Analysis of the results showed that the ZrO2 ceramic ball showed significantly better wear values when compared to the X46Cr13 stainless steel ball. It also improves the values of the coefficient of friction with respect to the type of wear that occurs when the experimental materials and counterparts are in contact.
The Effect of Pre-Annealing on the Evolution of the Microstructure and Mechanical Behavior of Aluminum Processed by a Novel SPD Method
Authors: Zhilyaev Alexander P. , Torres Mario J. , Cadena Homero D. , Rodriguez Sandra L. , Calvo Jessica , Cabrera José-María
Published: May 2020

A novel continuous process of severe plastic deformation (SPD) named continuous close die forging (CCDF) is presented. The CCDF process combines all favorite advances of multidirectional forging and other SPD methods, and it can be easily scaled up for industrial use. Keeping constant both the cross section and the length of the sample, the new method promotes a refinement of the microstructure. The grain refinement and mechanical properties of commercially pure aluminum (AA1050) were studied as a function of the number of CCDF repetitive passes and the previous conditioning heat treatment. In particular, two different pre-annealing treatments were applied. The first one consisted of a reheating to 623 K (350 °C) for 1 h aimed at eliminating the effect of the deformation applied during the bar extrusion. The second pre-annealing consisted on a reheating to 903 K (630 °C) for 48 h plus cooling down to 573 K (300 °C) at 66 K/h. At this latter temperature, the material remained for 3 h prior to a final cooling to room temperature within the furnace, i.e., slow cooling rate. This treatment aimed at increasing the elongation and formability of the material. No visible cracking was detected in the workpiece of AA1050 processed up to 16 passes at room temperature after the first conditioning heat treatment, and 24 passes were able to be applied when the material was subjected to the second heat treatment. After processing through 16 passes for the low temperature pre-annealed samples, the microstructure was refined down to a mean grain size of 0.82 µm and the grain size was further reduced to 0.72 µm after 24 passes, applied after the high temperature heat treatment. Tensile tests showed the best mechanical properties after the high temperature pre-annealing and 24 passes of the novel CCDF method. A yield strength and ultimate tensile strength of 180 and 226 MPa, respectively, were obtained. Elongation to fracture was 18%. The microstructure and grain boundary nature are discussed in relation to the mechanical properties attained by the current ultrafine-grained (UFG) AA1050 processed by this new method.
Influence of Pore-Size Distribution on the Resistance of Clay Brick to Freeze–Thaw Cycles
Authors: Netinger Grubeša Ivanka , Vračević Martina , Ranogajec Jonjaua , Vučetić Snežana
Published: May 2020

This study examines the influence of raw material characteristics, methods of shaping and of parameters of firing process of clay bricks, on pore-size distribution and on resistance to freeze–thaw cycles (with particular emphasis on the retention time of the specimens at the maximum achieved temperature). Pore-size distribution was measured by mercury-intrusion porosimetry, while the resistance to freeze–thaw cycles was assessed by exposing the bricks to freeze–thaw cycles (HRN B.D8.011 standard) monitoring the appearance of surface changes, decrease of compressive strength as well as the Maage factor. A correlation was set up between the Maage factor and the ratio of the compressive strength before and after freezing as a quantitative indicator of bricks resistance to frost. By using this correlation for all the examined bricks, regardless of their raw material and shaping procedure, a low coefficient of correlation (R2 = 0.26) was obtained. When processed separately, machine-made bricks had a significantly higher correlation coefficient value (R2 = 0.60) than the hand-made bricks (R2 = 0.28).
Musculoskeletal Multibody Simulation Analysis on the Impact of Patellar Component Design and Positioning on Joint Dynamics after Unconstrained Total Knee Arthroplasty
Authors: Kebbach Maeruan , Darowski Martin , Krueger Sven , Schilling Christoph , Grupp Thomas M. , Bader Rainer , Geier Andreas
Published: May 2020

Patellofemoral (PF) disorders are considered a major clinical complication after total knee replacement (TKR). Malpositioning and design of the patellar component impacts knee joint dynamics, implant fixation and wear propagation. However, only a limited number of studies have addressed the biomechanical impact of the patellar component on PF dynamics and their results have been discussed controversially. To address these issues, we implemented a musculoskeletal multibody simulation (MMBS) study for the systematical analysis of the patellar component’s thickness and positioning on PF contact forces and kinematics during dynamic squat motion with virtually implanted unconstrained cruciate-retaining (CR)-TKR. The patellar button thickness clearly increased the contact forces in the PF joint (up to 27%). Similarly, the PF contact forces were affected by superior–inferior positioning (up to 16%) and mediolateral positioning (up to 8%) of the patellar button. PF kinematics was mostly affected by the mediolateral positioning and the thickness of the patellar component. A medialization of 3 mm caused a lateral patellar shift by up to 2.7 mm and lateral patellar tilt by up to 1.6°. However, deviations in the rotational positioning of the patellar button had minor effects on PF dynamics. Aiming at an optimal intraoperative patellar component alignment, the orthopedic surgeon should pay close attention to the patellar component thickness in combination with its mediolateral and superior–inferior positioning on the retropatellar surface. Our generated MMBS model provides systematic and reproducible insight into the effects of patellar component positioning and design on PF dynamics and has the potential to serve as a preoperative analysis tool.
Evaluation of a Cell-Free Collagen Type I-Based Scaffold for Articular Cartilage Regeneration in an Orthotopic Rat Model
Authors: Szychlinska Marta Anna , Calabrese Giovanna , Ravalli Silvia , Dolcimascolo Anna , Castrogiovanni Paola , Fabbi Claudia , Puglisi Caterina , Lauretta Giovanni , Di Rosa Michelino , Castorina Alessandro , Parenti Rosalba , Musumeci Giuseppe
Published: May 2020

The management of chondral defects represents a big challenge because of the limited self-healing capacity of cartilage. Many approaches in this field obtained partial satisfactory results. Cartilage tissue engineering, combining innovative scaffolds and stem cells from different sources, emerges as a promising strategy for cartilage regeneration. The aim of this study was to evaluate the capability of a cell-free collagen I-based scaffold to promote cartilaginous repair after orthotopic implantation in vivo. Articular cartilage lesions (ACL) were created at the femoropatellar groove in rat knees and cell free collagen I-based scaffolds (S) were then implanted into right knee defect for the ACL-S group. No scaffold was implanted for the ACL group. At 4-, 8- and 16-weeks post-transplantation, degrees of cartilage repair were evaluated by morphological, histochemical and gene expression analyses. Histological analysis shows the formation of fibrous tissue, at 4-weeks replaced by a tissue resembling the calcified one at 16-weeks in the ACL group. In the ACL-S group, progressive replacement of the scaffold with the newly formed cartilage-like tissue is shown, as confirmed by Alcian Blue staining. Immunohistochemical and quantitative real-time PCR (qRT-PCR) analyses display the expression of typical cartilage markers, such as collagen type I and II (ColI and ColII), Aggrecan and Sox9. The results of this study display that the collagen I-based scaffold is highly biocompatible and able to recruit host cells from the surrounding joint tissues to promote cartilaginous repair of articular defects, suggesting its use as a potential approach for cartilage tissue regeneration.
Cobaloximes as Building Blocks in Halogen-Bonded Cocrystals
Authors: Bedeković Nikola , Martinez Valentina , Topić Edi , Stilinović Vladimir , Cinčić Dominik
Published: May 2020

In this work, we explore the halogen-bonded cocrystallization potential of cobaloxime complexes in the synthesis of cocrystals with perhalogenated benzenes. We demonstrate a strategy for synthesizing halogen-bonded metal–organic cocrystals by utilizing cobaloximes whose pendant bromide group and oxime oxygen enable halogen bonding. By combining three well-known halogen bond donor molecules differing in binding geometry and composition with three cobaloxime units, we obtained a total of four previously unreported cocrystals. Single crystal X-ray diffraction experiments showed that the majority of obtained cocrystals exhibited the formation of the targeted I···O and I···Br motives. These results illustrate the potential of cobaloximes as halogen bond acceptors and indicate that this type of halogen bond acceptors may offer a novel route to metal–organic halogen-bonded cocrystals.
Mechanical Behavior of Masonry Mortars Made with Recycled Mortar Aggregate
Authors: Mora-Ortiz René Sebastián , Munguía-Balvanera Emmanuel , Díaz Sergio Alberto , Magaña-Hernández Francisco , Del Angel-Meraz Ebelia , Bolaina-Juárez Álvaro
Published: May 2020

Recycling is an important habit to avoid waste. This paper evaluates the performance of masonry mortar, elaborated by replacing natural sand with recycled fine aggregate (RFA) obtained from mortar. Five families of mixtures were prepared with different replacement proportions: 20%, 40%, 60%, and 100%. A 1:4 volumetric cement-to-aggregate ratio was used for all mixtures by experimentally adjusting the amount of water to achieve the same consistency of 175 ± 5 mm. The effects of the following procedures were analyzed: (1) the use of a deconstruction technique to collect the RFA, (2) pre-wetting of the aggregates, and (3) the use of a commercial plasticizer. Experimental results show that it is possible to use this type of recycled fine aggregate as a substitute for natural sand by up to 60% in the manufacture of masonry mortar without significantly affecting its properties.
Microstructure and Low Cycle Fatigue Properties of AA5083 H111 Friction Stir Welded Joint
Authors: Torzewski Janusz , Grzelak Krzysztof , Wachowski Marcin , Kosturek Robert
Published: May 2020

The present paper aims to analyze the microstructure, microhardness, tensile properties, and low cycle fatigue (LCF) behavior of friction stir welded (FSW) butt joints. The material used in this study was the 5 mm thick 5083 H111 aluminum alloy sheet. Butt joints of AA 5083 H111 were manufactured at different operating parameters of the FSW process. The effect of the welding parameters on microstructure, microhardness, and tensile properties was investigated. Based on microstructure analysis and strength tests, the most favorable parameters of the FSW process were settled on the point of view of weld quality. Then, LCF tests of base material and friction stir welded specimens made of 5083 H111 were carried out for the examined welded samples under selected friction stir welding parameters. The process of low-cycle fatigue of 5083 H111 aluminum alloy was characterized by cyclic hardening for both: base material and FSW joint. It was revealed by a decrease in the width of the hysteresis loop with the simultaneous significant increase in the values of the range of stress. It was determined that fatigue cracks are initiated by cyclic slip deformation due to local stress concentration from the surface in the corner of the samples for the base material and the heat-affected zone for FSW joints. For all tested strain amplitudes, the fatigue crack propagation region is characterized by the presence of fatigue striation with secondary cracks.
Magnetostrictive Performance of Electrodeposited TbxDy(1−x)Fey Thin Film with Microcantilever Structures
Authors: Shim Hang , Sakamoto Kei , Inomata Naoki , Toda Masaya , Toan Nguyen Van , Ono Takahito
Published: May 2020

The microfabrication with a magnetostrictive TbxDy(1−x)Fey thin film for magnetic microactuators is developed, and the magnetic and magnetostrictive actuation performances of the deposited thin film are evaluated. The magnetostrictive thin film of TbxDy(1−x)Fey is deposited on a metal seed layer by electrodeposition using a potentiostat in an aqueous solution. Bi-material cantilever structures with the Tb0.36Dy0.64Fe1.9 thin-film are fabricated using microfabrication, and the magnetic actuation performances are evaluated under the application of a magnetic field. The actuators show large magnetostriction coefficients of approximately 1250 ppm at a magnetic field of 11000 Oe.
Two Faces of Vitamin C—Antioxidative and Pro-Oxidative Agent
Authors: Kaźmierczak-Barańska Julia , Boguszewska Karolina , Adamus-Grabicka Angelika , Karwowski Bolesław T.
Published: May 2020

Vitamin C has been known for decades. It is common in everyday use as an element of the diet, supplementation, and a preservative. For years, research has been conducted to precisely determine the mechanism of action of ascorbate in the cell. Available results indicate its multi-directional cellular effects. Vitamin C, which belongs to antioxidants scavenging free radicals, also has a ‘second face’—as a pro-oxidative factor. However, whether is the latter nature a defect harmful to the cell, or whether a virtue that is a source of benefit? In this review, we discuss the effects of vitamin C treatment in cancer prevention and the role of ascorbate in maintaining redox balance in the central nervous system (CNS). Finally, we discuss the effect of vitamin C supplementation on biomarkers of oxidative DNA damage and review the evidence that vitamin C has radioprotective properties.
Dairy Products Quality from a Consumer Point of View: Study among Polish Adults
Authors: Sajdakowska Marta , Gębski Jerzy , Guzek Dominika , Gutkowska Krystyna , Żakowska-Biemans Sylwia
Published: May 2020

The aims of the current study were (a) to deepen the understanding of food quality from animal origin with particular emphasis on dairy products, including yoghurt; (b) to determine the level of acceptance of methods and ingredients used to enhance the quality of food from animal origin; (c) to identify how the perception of animal products quality affects the acceptance of changes in production methods and (d) to identify the projective image of consumers purchasing high-quality yoghurt. The data were collected using a CAPI (Computer Assisted Personal Interview) survey on a sample of 983 consumers. The k-means clustering method (k-means clustering algorithm is an unsupervised algorithm that is used to segment the interest area from the background) was used to identify five clusters of consumers. Moreover, the logistic regression models were used in order to examine the impact of opinions related to the quality of product on acceptance of food production methods. The results showed that food quality is generally perceived by consumers using the following attributes: its freshness, naturalness, production method, as well as appearance, taste and smell, but when it comes to the quality of food from animal origin, convenience, connected with the availability, nutritional value and health benefits is of primary importance. The most accepted production method of high-quality food is animal production that takes into consideration the welfare of farm animals. Results also show that the increase in the level of education among the surveyed people contributed to the acceptance of ensuring welfare of farm animals as a method of increasing food quality while consumers′ openness to new products favored the acceptance of adding health-promoting ingredients to livestock feed. As regards the assessment of the level of acceptance of enhancing food with beneficial ingredients, people for whom health aspects were important declared their willingness to accept such a method of increasing food quality. The research findings can be used to develop educational campaigns as well as marketing communication of enterprises operating on the food market. Furthermore, the results could be used to strengthen the competitive position of food enterprises searching for innovative solutions.
Wound Healing Activity of Nanoclay/Spring Water Hydrogels
Authors: García-Villén Fátima , Faccendini Angela , Miele Dalila , Ruggeri Marco , Sánchez-Espejo Rita , Borrego-Sánchez Ana , Cerezo Pilar , Rossi Silvia , Viseras César , Sandri Giuseppina
Published: May 2020

Background: hydrogels prepared with natural inorganic excipients and spring waters are commonly used in medical hydrology. Design of these clay-based formulations continues to be a field scarcely addressed. Safety and wound healing properties of different fibrous nanoclay/spring water hydrogels were addressed. Methods: in vitro biocompatibility, by means of MTT assay, and wound healing properties were studied. Confocal Laser Scanning Microscopy was used to study the morphology of fibroblasts during the wound healing process. Results: all the ingredients demonstrated to be biocompatible towards fibroblasts. Particularly, the formulation of nanoclays as hydrogels improved biocompatibility with respect to powder samples at the same concentration. Spring waters and hydrogels were even able to promote in vitro fibroblasts motility and, therefore, accelerate wound healing with respect to the control. Conclusion: fibrous nanoclay/spring water hydrogels proved to be skin-biocompatible and to possess a high potential as wound healing formulations. Moreover, these results open new prospects for these ingredients to be used in new therapeutic or cosmetic formulations.
Osteoprotegerin Is more than a Possible Serum Marker in Liver Fibrosis: A Study into Its Function in Human and Murine Liver
Authors: Adhyatmika Adhyatmika , Beljaars Leonie , Putri Kurnia S. S. , Habibie Habibie , Boorsma Carian E. , Reker-Smit Catharina , Luangmonkong Theerut , Guney Burak , Haak Axel , Mangnus Keri A. , Post Eduard , Poelstra Klaas , Ravnskjaer Kim , Olinga Peter , Melgert Barbro N.
Published: May 2020

Osteoprotegerin (OPG) serum levels are associated with liver fibrogenesis and have been proposed as a biomarker for diagnosis. However, the source and role of OPG in liver fibrosis are unknown, as is the question of whether OPG expression responds to treatment. Therefore, we aimed to elucidate the fibrotic regulation of OPG production and its possible function in human and mouse livers. OPG levels were significantly higher in lysates of human and mouse fibrotic livers compared to healthy livers. Hepatic OPG expression localized in cirrhotic collagenous bands in and around myofibroblasts. Single cell sequencing of murine liver cells showed hepatic stellate cells (HSC) to be the main producers of OPG in healthy livers. Using mouse precision-cut liver slices, we found OPG production induced by transforming growth factor β1 (TGFβ1) stimulation. Moreover, OPG itself stimulated expression of genes associated with fibrogenesis in liver slices through TGFβ1, suggesting profibrotic activity of OPG. Resolution of fibrosis in mice was associated with decreased production of OPG compared to ongoing fibrosis. OPG may stimulate fibrogenesis through TGFβ1 and is associated with the degree of fibrogenesis. It should therefore be investigated further as a possible drug target for liver fibrosis or biomarker for treatment success of novel antifibrotics.
Improving the Accuracy of Automatic Reconstruction of 3D Complex Buildings Models from Airborne Lidar Point Clouds
Authors: Kulawiak Marek , Lubniewski Zbigniew
Published: May 2020

Due to high requirements of variety of 3D spatial data applications with respect to data amount and quality, automatized, efficient and reliable data acquisition and preprocessing methods are needed. The use of photogrammetry techniques—as well as the light detection and ranging (LiDAR) automatic scanners—are among attractive solutions. However, measurement data are in the form of unorganized point clouds, usually requiring transformation to higher order 3D models based on polygons or polyhedral surfaces, which is not a trivial process. The study presents a newly developed algorithm for correcting 3D point cloud data from airborne LiDAR surveys of regular 3D buildings. The proposed approach assumes the application of a sequence of operations resulting in 3D rasterization, i.e., creation and processing of a 3D regular grid representation of an object, prior to applying a regular Poisson surface reconstruction method. In order to verify the accuracy and quality of reconstructed objects for quantitative comparison with the obtained 3D models, high-quality ground truth models were used in the form of the meshes constructed from photogrammetric measurements and manually made using buildings architectural plans. The presented results show that applying the proposed algorithm positively influences the quality of the results and can be used in combination with existing surface reconstruction methods in order to generate more detailed 3D models from LiDAR scanning.
Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors
Authors: Samanta Anirban , Medintz Igor L.
Published: May 2020

Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect: that the excited donor here is produced by biochemical means and not by an external illumination. Often the choice of BRET source is the bioluminescent protein Renilla luciferase, which catalyzes the oxidation of a substrate, typically coelenterazine, producing an oxidized product in its electronic excited state that, in turn, couples with a proximal fluorophore resulting in a fluorescence emission from the acceptor. The acceptors pertinent to this discussion are semiconductor quantum dots (QDs), which offer some unrivalled photophysical properties. Amongst other advantages, the QD’s large Stokes shift is particularly advantageous as it allows easy and accurate deconstruction of acceptor signal, which is difficult to attain using organic dyes or fluorescent proteins. QD-BRET systems are gaining popularity in non-invasive bioimaging and as probes for biosensing as they don’t require external optical illumination, which dramatically improves the signal-to-noise ratio by avoiding background auto-fluorescence. Despite the additional advantages such systems offer, there are challenges lying ahead that need to be addressed before they are utilized for translational types of research.
Spectral Analysis of Electricity Demand Using Hilbert–Huang Transform
Authors: Luque Joaquin , Anguita Davide , Pérez Francisco , Denda Robert
Published: May 2020

The large amount of sensors in modern electrical networks poses a serious challenge in the data processing side. For many years, spectral analysis has been one of the most used approaches to extract physically meaningful information from a sea of data. Fourier Transform (FT) and Wavelet Transform (WT) are by far the most employed tools in this analysis. In this paper we explore the alternative use of Hilbert–Huang Transform (HHT) for electricity demand spectral representation. A sequence of hourly consumptions, spanning 40 months of electrical demand in Spain, has been used as dataset. First, by Empirical Mode Decomposition (EMD), the sequence has been time-represented as an ensemble of 13 Intrinsic Mode Functions (IMFs). Later on, by applying Hilbert Transform (HT) to every IMF, an HHT spectrum has been obtained. Results show smoother spectra with more defined shapes and an excellent frequency resolution. EMD also fosters a deeper analysis of abnormal electricity demand at different timescales. Additionally, EMD permits information compression, which becomes very significant for lossless sequence representation. A 35% reduction has been obtained for the electricity demand sequence. On the negative side, HHT demands more computer resources than conventional spectral analysis techniques.
Self-Powered Sensors and Systems Based on Nanogenerators
Authors: Wu Zhiyi , Cheng Tinghai , Wang Zhong Lin
Published: May 2020

Sensor networks are essential for the development of the Internet of Things and the smart city. A general sensor, especially a mobile sensor, has to be driven by a power unit. When considering the high mobility, wide distribution and wireless operation of the sensors, their sustainable operation remains a critical challenge owing to the limited lifetime of an energy storage unit. In 2006, Wang proposed the concept of self-powered sensors/system, which harvests ambient energy to continuously drive a sensor without the use of an external power source. Based on the piezoelectric nanogenerator (PENG) and triboelectric nanogenerator (TENG), extensive studies have focused on self-powered sensors. TENG and PENG, as effective mechanical-to-electricity energy conversion technologies, have been used not only as power sources but also as active sensing devices in many application fields, including physical sensors, wearable devices, biomedical and health care, human–machine interface, chemical and environmental monitoring, smart traffic, smart cities, robotics, and fiber and fabric sensors. In this review, we systematically summarize the progress made by TENG and PENG in those application fields. A perspective will be given about the future of self-powered sensors.
Sustainable Soil and Water Resources Management in Nigeria: The Need for a Data-Driven Policy Approach
Authors: Doro Kennedy , Ehosioke Solomon , Aizebeokhai Ahzegbobor
Published: May 2020

Effective public policies are needed to manage a nation’s natural resources, including soil and water. However, making such policies currently requires a shift from a traditional qualitative approach to a mix of scientific data, evidence and the relevant social elements, termed data-driven policymaking. Nigeria, like most developing countries, falls short of the framework for this approach. Nevertheless, the lack of potable water in some regions and the continuous degradation of farmable lands call for intervention through effective policy formulation and implementation. In this work, we present a conceptual workflow as a strategic step towards developing a framework for a data-driven soil and water resources management policy. A review of the current legal and policy framework and selected scientific literature on soil and water resources in Nigeria is presented. Analysis of the National Water Resources Bill proposed in 2018 is used to highlight existing gaps between policy, scientific data and reality. Modern field techniques and project-based examples for soil and aquifer characterization that can be adapted for local use are presented. While government must take responsibility for the poor policy framework, the research community is challenged on the need for scientific data as a base for effective policy formulation and implementation.
Responses of Rural Households to the Cotton Crisis in Benin
Authors: Maboudou Alidou Guirguissou , Niehof Anke
Published: May 2020

Relying on one source of income puts the livelihood system of rural households at risk. In Benin, cotton has long been the core cash crop of rural livelihood systems—until the mid-2000s, when multiple constraints led to the demise of cotton production. This paper investigates the responses of rural households to the economic shock resulting from the collapse of the cotton sector and the consecutive decrease of income from cotton. The primary data collection was carried out between 2009 and 2012 and included a household survey and focus group discussions with groups of farmers. The results reveal that households diversified their sources of income on farm, with food crops increasingly gaining a cash function. However, because the production system still depended heavily on cotton for access to fertilizers and other inputs for food crops, farmers continued to grow cotton despite its decreasing returns. In addition, because of their multiple extra-domestic activities, women seemed to be less vulnerable than men when coping with livelihood shocks; indeed, their contribution to providing for household needs increased. Further results revealed that young men devised their own ways of dealing with the crisis by temporary migration.
Reducing Groundwater Contamination from On-Site Sanitation in Peri-Urban Sub-Saharan Africa: Reviewing Transition Management Attributes towards Implementation of Water Safety Plans
Authors: Twinomucunguzi Felix R. B. , Nyenje Philip M. , Kulabako Robinah N. , Semiyaga Swaib , Foppen Jan Willem , Kansiime Frank
Published: May 2020

High urbanization in Sub-Saharan Africa (SSA) has resulted in increased peri-urban groundwater contamination by on-site sanitation. The World Health Organization introduced Water Safety Plans (WSP) towards the elimination of contamination risks to water supply systems; however, their application to peri-urban groundwater sources has been limited. Focusing on Uganda, Ghana, and Tanzania, this paper reviews limitations of the existing water regime in addressing peri-urban groundwater contamination through WSPs and normative attributes of Transition Management (TM) towards a sustainable solution. Microbial and nutrient contamination remain prevalent hazards in peri-urban SSA, arising from on-site sanitation within a water regime following Integrated Water Resources Management (IWRM) principles. Limitations to implementation of WSPs for peri-urban groundwater protection include policy diversity, with low focus on groundwater; institutional incoherence; highly techno-centric management tools; and limited regard for socio-cultural and urban-poor aspects. In contrast, TM postulates a prescriptive approach promoted by community-led frontrunners, with flexible and multi-domain actors, experimenting through socio-technical tools towards a shared vision. Thus, a unified risk-based management framework, harnessing attributes of TM and IWRM, is proposed towards improved WSP implementation. The framework could assist peri-urban communities and policymakers in formulating sustainable strategies to reduce groundwater contamination, thereby contributing to improved access to safe water.
Corporate Sustainability Management, Earnings Transparency, and Chaebols
Authors: Oh Hyun Min , Park Sam Bock , Ma Hee Young
Published: May 2020

This study examines the association of corporate sustainability management with earnings transparency. Based on previous studies that indicate that sustainability management activities reduce earnings management and corporate risk and increase a firm’s value, this study predicts that the firms with effective sustainability management will have a high earnings transparency. In addition, this study examines the differential effect of corporate sustainability management on earnings transparency according to whether or not a firm belongs to a chaebol. We use Environmental, Social, and Governance (ESG) ratings of the Korean Corporate Governance Service (KCGS) as a proxy for corporate sustainability management and apply the method of Cheng and Subramanyam (2008) to measure earnings transparency. The empirical results show that there is a significant positive relationship between corporate sustainability management and earnings transparency. Furthermore, the association between corporate sustainability management and earnings transparency is more negative for firms belonging to a chaebol. These results indirectly show that firms belonging to a chaebol have a lower level of information asymmetry than firms not belonging to a chaebol. This study focuses on corporate sustainability management as a determinant of earnings transparency, and is useful for examining the effect of belonging to a chaebol on the relationship between sustainability management and earnings transparency. Our results are expected to provide important implications not only for managers, but also for investors and regulators.
Consumers’ Evaluation of Stockfree-Organic Agriculture—A Segmentation Approach
Authors: Jürkenbeck Kristin , Spiller Achim
Published: May 2020

Recently, more and more research has been conducted on what sustainable nutrition could look like. Stockfree-organic agriculture is one possible approach but a relatively new and unstudied cultivation method. In addition to organic agriculture, it excludes any animal by-products during the whole cultivation process. Among the consumers of organic food are especially many vegetarians and vegans. To attract this target group, first farms in Europe have started to follow the stockfree-organic agriculture principles. As it is important to know the consumers’ point of view on new developments in agriculture at an early stage of the diffusion process, this study deals with consumers’ evaluation of stockfree-organic agriculture to draw conclusions about a possible market potential. This is especially important for stockfree-organic farmers, as well as for organic farmers who are considering converting their cultivation method, and for retailers who wonder whether it is worthwhile to offer these products. The data was collected in 2019 by means of an online survey. The sample consisted of 500 German respondents. Principal component and cluster analyses were used to identify consumer segments according to their attitudes towards the acceptance, advantages, and disadvantages of stockfree-organic agriculture. Additionally, the different segments were compared with each other according to various attitudes and eating behaviours. Overall, animal welfare considerations and environmental aspects were of particular importance to consumers. Animal usage was clearly rejected by one segment, which contained 24% of the sample. Nearly all vegetarians and all vegans supported stockfree-organic agriculture, whereas heavy meat consumers tended to refuse the support of stockfree-organic agriculture. The supporting group valuing high animal welfare and health concerns was much larger than the current status of this niche segment would suggest. This could be a major challenge for the agricultural sector in the long term, but could also include opportunities for greater sustainability.