bluuebunny/crossref_metadata_2025 huggingface dataset:
Meningkatkan Pengetahuan Remaja dalam Penelitian Sosial di Masa Pandemi
Authors: Kurniawan Nandi , Saipiatuddin Saipiatuddin , Hidayaht Achmad
Published: June 2022

This article is the result of community service activities that aim to increase adolescent knowledge in social research, especially during the Covid 19 pandemic. The form of activity is in the form of webinars which are expected to be one solution to overcome the low interest of adolescents in social research. This community service activity was carried out in the youth scientific group (KIR) of SMAN 24 which consisted of 15 student members. The webinar activity was carried out in two sessions of material, among others about the urgency of social research and types of social research. The material is provided with an interactive lecture and discussion model so that the activities are two-way. Data collection was carried out in the form of an open questionnaire and pre-test-post-test. Data analysis with descriptive statistics and analysis of answers from open questionnaires. The results of the activity show that KIR member students have understood the urgency of social research and are able to express ideas to be developed into social research. Social research can be a positive activity for teenagers during a pandemic as a medium for self-development. In conclusion, this activity has been able to increase students' knowledge and understanding in social research. In addition, this activity has also developed critical thinking skills.

ABSTRAK

Artikel ini merupakan hasil dari kegiatan pengabdian kepada masyarakat yang bertujuan untuk meningkatkan pengetahuan remaja dalam penelitian sosial terutama di masa pandemic Covid 19. Bentuk kegiatan berupa webinar yang diharapkan dapat menjadi salah satu solusi untuk mengatasi rendahnya minat remaja dalam penelitian sosial. Kegiatan pengabdian masyarakat ini dilaksanakan pada kelompok ilmiah remaja (KIR) SMAN 24 yang terdiri dari 15 siswa anggota. Kegiatan webinar dilaksanakan dalam dua sesi materi antara lain tentang urgensi penelitian sosial dan jenis - jenis penelitian sosial. Materi diberikan dengan model ceramah interaktif dan diskusi agar kegiatan bersifat dua arah. Pengumpulan data dilakukan berupa angket terbuka dan pre test-post test. Analisis data dengan statistik deksriptif dan analisis jawaban dari angket terbuka. Hasil kegiatan menunjukan siswa anggota KIR telah memahami urgensi penelitian sosial dan mampu mengungkapkan ide untuk dikembangkan ke dalam penelitian sosial. Penelitian sosial dapat menjadi kegiatan positif bagi remaja pada saat pandemi sebagai media pengembangan diri. Kesimpulan dari kegiatan ini telah mampu menigkatkan pengetahuan dan pemahaman siswa dalam penelitian sosial. Selain itu kegiatan ini juga telah mengembangkan kemampuan berpikir kritis.
Pengembangan Literasi Kewirausahaan Digital Pada Komunitas Dasawisma, Kampung Baru Nelayan, Cilincing, Jakarta Utara
Authors: Hamid Abdul Rahman , Septiandini Devi , Siswanto Achmad , Suyuti Suyuti
Published: June 2022

ABSTRAK

Tujuan kegiatan ini adalah untuk memberikan pengetahuan serta keterampilan kepada masyarakat Kampung Baru Nelayan, Cilincing, Jakarta Utara dalam mengembangkan literasi kewirausahaan digital. Kegiatan inti dilaksanakan di RT 007/RW 08 pada tanggal 13 November 2021. Metode yang digunakan yakni berupa pemaparan materi, diskusi dan praktik berupa demo memasak “rice bowl” sebagai bentuk dari literasi kewirausahaan digital. Kegiatan ini menjaring 20 orang ibu – ibu dari komunitas Dasawisma dan juga melibatkan mahasiswa dari program studi pendidikan sosiolgi dan program studi pendidikan tata boga sebanyak 5 orang. Hasil yang diperoleh dari kegiatan ini adalah semakin meningkatnya pengetahuan masyarakat mengenai literasi kewirausahaan digital melalui beberapa platform media sosial yang sudah familiar seperti Whatsapp, Facebook dan Instagram. Selain platfom media sosial tersebut, peserta juga diajarkan bagaimana mendaftarkan usahanya di platform seperti Go Food dan Grab Food. Pelatihan ini juga meningkatkan keterampilan memasak para peserta lewat demo masak yang diberikan oleh mahasiswa dengan memanfaatkan potensi lokal yakni menu berbahan dasar Kerang.

 

ABSTRACT

The purpose of this activity is to provide knowledge and skils to the people of Kampung Baru Nelayan, Cilincing,Jakarta Utara. In developing digital enterpreneurship literacy. The activity was held in RT 007/RW 08 on November 13, 2021. The method used was in the form of material presentation , discussion and practice in the form of a “rice bowl” cooking demonstration as a form of digital enterpreneurship literacy. The activity recruited 20 women from the Dasawisma community and also involved 5 students from Pendidikan Sosiologi and Pendidikan Tata Bog. The result obtained from this activity are increasing public knowledge about digital enterpreneurship literacy through several familiar social nedia platform such as WhatsApp, Facebook and Instagram. In addition to these social media platform, participants were also taught how to register their businesses on platforms such as Go Food and Grab Food. This training improves the cooking skills of the participants trough cooking demonstrations given by students by utilizing local potential, namely the menu made from shellfish.
Pendampingan Penulisan Toponim Kabupaten Karawang Untuk Guru Sejarah di Karawang
Authors: Humaidi Humaidi , Umasih Umasih , Fakhruddin M. , Abrar Abrar , Yanuardi M. Hasmi , Wisnubroto R. , Santosa Firdaus Hadi
Published: June 2022

ABSTACT

This Community Service Activity is an activity based on community assistance to write and produce a local history product for its own environment. This is based on an analysis of the situation and needs of the people who do not have historical records and only rely on memories.

The method used is service learning, which is divided into two activities that are synchronous and asynchronous. Synchronous consists of delivering material and presentations, while asynchronous is in the form of guidance on writing Karawang toponyms. The participants who took part in this Community Service Activity were 62 history teachers who produced a history book on the toponym of Karawang.

The results of this dedication show that history teachers have a strong interest in toponymy or local history. The process of finding sources, both literature study and field visits, provides an experience of researching history that strengthens soft skills in historical research.

 

ABSTRAK

Kegiatan Pengabdian Kepada Masyarakat ini adalah sebuah kegiatan yang berbasis pendampingan masyarakat untuk menulis dan menghasilkan sebuah produk sejarah lokal lingkungannya sendiri. Hal ini didasarkan atas sebuah analisis situasi kebutuhan masyarakat yang belum memiliki catatan sejarahnya dan hanya mengandalkan ingatan saja.

Metode yang digunakan  adalah service learning yang terbagi  menjadi  dua  kegiatan  yaitu synchronous dan asynchronous. Anchronous terdiri atas penyampaian materi dan presentasi, sedangkan asynchronous berupa bimbingan penulisan toponim karawang. Peserta yang mengikuti kegiatan PkM ini adalah sebanyak 62 guru sejarah yang menghasilkan sebuah buku sejarah mengenai toponim Karawang.

Hasil pengabdian  ini menunjukkan bahwa para guru sejarah memiliki ketertarikan yang kuat terhadap toponimi atau sejarah local. Proses pencarian sumber, baik study literatur dan kunjungan lapangan, memberikan sebuah pengalaman meneliti sejarah yang memperkuat sofskill dalam penelitian sejarah.
Penguatan Religiusitas di Masa Pandemik Melalui Kajian Kitab Fathul Qorib
Authors: Narulita Sari , Aulia Rihlah Nur , Kurniati Devi
Published: June 2022

ABSTRAK

Larangan berkumpul di masa pandemic membuat semangat keberagamaan banyak muslim menurun; hal ini dikarenakan kaum muslim menjadi sulit untuk mengikuti berbagai kajian yang biasa diikuti, terlebih kajian Fiqh, yang terkait dengan aturan dalam beribadah. Berdasarkan hal tersebut, Prodi Pendidikan Agama Islam bekerjasama dengan MATAN UNJ mengadakan pembelajaran Fiqh secara daring, membedah Kitab Fathul Qarib.  Kegiatan ini dilakukan sekali di setiap bulannya dalam rentang Januari – Agustus 2021. Evaluasi kegiatan menunjukkan antusias peserta dalam mengikuti kegiatan,terlebih paparan materi tidak hanya Terpaku pada konten kitab Fathul Qarib yang merupakan kitab klasik; namun juga di korelasikan dengan permasalahan yang ada pada masa kini. Peserta mendapatkan pemahaman agama yang mumpuni walaupun kajiannya dilakukan secara daring.

 

 

ABSTRACT

The prohibition of gathering during the pandemic has reduced the religious spirit of many Muslims; This is because Muslims find it difficult to follow the various studies that are commonly followed, especially the study of Fiqh, which is related to the rules of worship. Based on this, the Islamic Religious Education Department in collaboration with MATAN UNJ held an online Fiqh study, dissecting the Book of Fathul Qarib. This activity is carried out once every month in the range of January – August 2021. The evaluation of the activities shows the enthusiasm of the participants in participating in the activity, especially the material exposure is not only focused on the content of the Fathul Qarib book which is a classic book; but also correlated with the problems that exist today. Participants gain a good understanding of religion even though the study is conducted online.
Literasi digital berbasis edukasi dalam pemanfaatan gadget di kalangan Kelompok Majelis Taklim pada masa pandemi
Authors: Sutjipto Vera Wijayanti , Putri Maulina Larasati , Sary Marisa Puspita , Putri Angieta Dewati , Novanra Fahira
Published: June 2022

ABSTRAK

Internet menyebabkan perubahan gaya hidup, sebagian besar masyarakat semakin pandai dalam mengoperasikan gadget dan memanfaatkan internet. Namun, disayangkan bahwa kepandaian dalam menggunakan gadget, tidak diiringi oleh kemampuan literasi digital. Padahal mempunyai pengetahuan literasi digital sangatlah penting, terutama saat pandemi ini, dimana hampir seluruh kegiatan masyarakat dilakukan secara daring (online), sehingga literasi digital telah menjadi keterampilan utama untuk mendapatkan informasi, mengembangkan pribadi berkomunikasi, dan berinteraksi dengan orang lain. Metode yang peneliti gunakan untuk penelitian ini adalah kualitatif dengan jenis penelitian yang digunakan deskriptif. Data yang digunakan sebagai bahan penelitian, dikumpulkan dengan cara melakukan wawancara dengan para informan, dokumentasi untuk menguatkan validasi data, dan observasi terhadap lingkungan disekitar informan serta tiap individu yang tergabung dalam Kelompok Majelis Taklim. Informan pada penelitian ini berjumlah delapan orang dan merupakan anggota dari Kelompok Majelis Taklim.

 

ABSTRACT

The internet causes lifestyle changes, most people are getting smarter in operating gadgets and using the internet. However, it is unfortunate that cleverness in using gadgets is not accompanied by digital literacy skills. Even though having knowledge of digital literacy is very important, especially during this pandemic, where almost all community activities are carried out online, so digital literacy has become the main skill to get information, develop personal communication, and interact with other people. The method that the researcher uses for this research is qualitative with the type of research used is descriptive. The data used as research material was collected by conducting interviews with informants, documentation to strengthen data validation, and observations of the environment around the informants and each individual who is a member of the Majelis Taklim Group. There were eight informants in this study and were members of the Majelis Taklim Group.
Luteryen-Katolik Diyaloğu: Aklanma Doktrini Ortak Bildirisi
Authors: ŞAHİN Osman
Published: June 2022

Hıristiyanlık tarihi ayrılık hikâyeleri ile doludur. Bu ayrılıkları gidermek için çeşitli kiliseler arasında birtakım diyalog faaliyetleri gerçekleşmiştir. Özellikle 19. yüzyılda bu çerçevede yeni bir canlanma yaşanmış ve 20. yüzyılda da bu tür girişimler artarak devam etmiştir. Roma Katolik Kilisesi başlangıçta bu tür çalışmalara karşı çoğunlukla mesafeli bir tutum takınmıştır. Ancak II. Vatikan Konsili’nden sonra Roma Kilisesi de ökümenik diyalog faaliyetlerine aktif bir şekilde katılmaya başlamıştır. Bu çerçevede Roma Katolik Kilisesi ile Luteryen Kiliseler arasında bir takım diyalog çalışmaları yürütülmüş ve bu iki kilise arasındaki en önemli teolojik sorun olan “aklanma doktrini” üzerine ortak bir bildiri yayımlanmıştır. Bu çalışma, Roma Katolik Kilisesi ile Luteryen Kiliseler arasında imzalanan “Aklanma Doktrini Ortak Bildirisi’nin” nasıl bir süreç sonunda ortaya çıktığını, içeriğini ve önemini açıklamayı hedeflemektedir.
Характеристика штаммов генетических вариантов вируса SARS-CoV-2, выделенных на территории РФ в 2020-2022 гг (Обзор)
Authors: Шиповалов Андрей , Кудров Глеб , Старчевская Мария , Пьянков Олег
Published: June 2022

С момента выявления первых случаев инфицирования в городе Ухань (Китай) вирусом SARS-CoV-2 и по сегодняшний день учреждениями Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (Роспотребнадзора) отслеживаются у вновь выявляемых вариантов изменения в геноме, способные привести к появлению новых нежелательных для человека свойств. На всем протяжении пандемии основными остаются методы молекулярно-биологического мониторинга, позволяя в кратчайшие сроки выявить мутации в клинически значимых регионах генома коронавируса. Предсказание методами молекулярного моделирования влияния единичных аминокислотных замен в участках связывания поверхностных белков коронавируса с клеточными рецепторами позволяет оценить эпидемическую значимость вновь выявляемых генетических вариантов, несущих те или иные значимые мутации. Подтвердить связь биологических свойств штаммов, относящихся к определенному генетическому варианту, с изменениями в структуре рецептор-связывающего домена S-белка коронавируса возможно только с помощью вирусологических методов исследования. В связи с этим настоящая работа имела целью представление свойств in vitro и in vivo выделенных на территории РФ штаммов коронавируса в зависимости от принадлежности к определенному генетическому варианту. Характеристика исследованных штаммов, полученная с использованием всех доступных методов, позволяет адекватно оценивать эпидемический потенциал каждого варианта вируса SARS-CoV-2.
Aplicación de los principios de la bioconstrucción (Baubiologie) como alternativa para el desarrollo del habitat rural sostenible en el diseño de una finca agroturistica
Authors:   , Cusicuna Palma Nohely Angela
Published: June 2022

La presente investigación tiene como finalidad determinar la influencia de los principios de la bioconstrucción para contribuir con el desarrollo del hábitat rural sostenible en el distrito de Pachacamac, Casa Blanca. El tipo de investigación es descriptiva correlacional la cual establece relaciones entre las dos variables precisando el sentido de causalidad de las mismas. Para ello se utilizaron los siguientes instrumentos metodológicos: Para la variable independiente (Los principios de la bioconstrucción) se utilizó la revisión bibliográfica, ya que mucha de la información requerida se encontró en libros, revistas y artículos. Además, se realizó un cuestionario de 5 preguntas en las cuales se midió la percepción de las personas con respecto a los materiales naturales y de construcción. Para la variable independiente (El hábitat rural sostenible) se desarrolló un cuestionario de 5 preguntas en las cuales se midieron la interacción y el sentido de pertenencia de la población con respecto al medio rural agrícola. También se realizó una entrevista a uno de los promotores de la bioconstrucción en Lima (Fundador de Tierra Langla) y una ficha de observación donde se analizaron las condiciones del hábitat rural y de las extensiones agrícolas del distrito. Se concluyó que la aplicación de la bioconstrucción contribuye a generar un desarrollo del hábitat rural sostenible, basándose en que las condiciones de confort espacial en una vivienda es un requisito fundamental para una calidad de vida adecuada y el uso de los ecomateriales permite dicha condición, además de generar una buena integración de la edificación con el paisaje.
Correlation between preoperative body composition and postoperative muscle volume and degeneration after total hip arthroplasty
Authors: Ukai Taku , Yokoyama Katsuya , Watanabe Masahiko
Published: June 2022

Background
Impaired muscle recovery after total hip arthroplasty (THA) may greatly affect gait and activities of daily living. Bioelectrical impedance analysis (BIA) may be used to assess body composition and muscle volume and computed tomography (CT) may be used for assessing muscle volume and, using Hounsfield units, fatty degeneration of muscle. This study aimed to assess the correlation between preoperative body composition and postoperative muscle volume and degeneration after THA using BIA and CT.
Methods
Thirty-eight patients who underwent THA and had BIA and CT performed both pre- and postoperatively were retrospectively assessed. Body composition measurements, specifically the fat mass index, fat-free mass index, and phase angle, were recorded using BIA. Pre- and postoperative muscle volume and Hounsfield units of the gluteus maximus and quadriceps of the affected hip were measured using CT. The correlation between preoperative body composition measurements and postoperative muscle volume and Hounsfield units of the gluteus maximus and quadriceps was evaluated.
Results
Preoperative fat mass index negatively correlated with postoperative muscle volume of the gluteus maximus (P = 0.02) and quadriceps (P < 0.001) and the Hounsfield units of the gluteus maximus (P = 0.03) and quadriceps (P = 0.03). Preoperative fat-free mass index positively correlated with postoperative muscle volume of the quadriceps (P = 0.02). Preoperative phase angle positively correlated with postoperative muscle volume of the quadriceps (P = 0.001) and the Hounsfield units of the gluteus maximus (P = 0.03) and quadriceps (P = 0.001).
Conclusion
In patients who underwent THA, preoperative body composition correlated with postoperative muscle volume and fatty degeneration of the affected lower limb. Preoperative body composition may be useful for predicting postoperative muscle volume and fatty degeneration and thus the extent of postoperative recovery.
Pre-hospital care providers’ perspective and associated factors towards pre-hospital emergency care in Addis Ababa, Ethiopia, 2022
Authors: Mamo Yohannes Feleke , H/mariam Hana Abera , Tura Teshome Gishu
Published: June 2022

Background: The pre-hospital emergency care is a system that provides emergency care to critically ill and injured patients in the field. Pre-hospital emergency care aims to deliver care on time to sufferers of sudden, life-threatening injuries and avoid unnecessary death or long-term morbidity. Accidental trauma, cardiac arrest, stroke, and obstetric emergencies are common causes of premature mortality and disability in low and middle-income countries. In these countries, most early deaths are the result of inadequate pre-hospital care provision.The objective of this study was to assess pre-hospital care providers' perspectives and associated factors toward pre-hospital emergency medical care.Result: In this study, the majority of participants' work experience was from 6-10years 40.6%(71), and 20%(35) above 10-years of work experience. The majority of care providers in the prehospital institution were 79.4% (139) nurses and 8.6% (15) emergency and critical nurses. The finding of this study showed that 96 (54.9%) of study participants had a good perspective and 45.1%(79) of the study participants had a poor perspective toward the pre-hospital emergency care. More than half of the participants of the study 52%(91) agreed that there is no proper documentation in prehospital care provider institutions. This study also indicated that there were no adequate basic life support equipment and drugs to provide emergency care in an ambulance. Eighty-seven (49.7%) of study participants replied that there were no the pre-hospital care guidelines and protocols that help care providers to give appropriate care in the ambulance serviceConclusion: The finding of this study showed that more than half of the study participants had a good perspective on the hospital emergency care. Pre-hospital care providers who had master's educational status were 1.17 times more likely to have a good perspective towards pre-hospital emergency care as compared to pre-hospital care providers who had diploma educational status. Therefore, the ministry of health of Ethiopia, the Addis Ababa health bureau, and all other stakeholders have to increase the training level of pre-hospital care providers to increase the quality care of prehospital service.
Effect of Pilates exercises with celery seed on antinuclear antibody levels in middle-aged women
Authors: Rostamkolai Touba Ahmadi , Nasiri Esmaeil
Published: June 2022

The aim of this study was to investigate the effect of Pilates training with celery seed supplementation on middle-aged women with rheumatoid arthritis to determine its effecting on the Antinuclear Antibodies levels (ANA) rheumatoid arthritis patients. Forty women with rheumatoid arthritis with mean age (53.80 ± 3.71) were randomly divided into four groups: control group, supplement group, exercise group and exercise group with celery seed consumption and each group with 10 replicates (N = 10). The exercise and exercise groups performed celery seed training for 8 weeks and 3 sessions per week for (30-60) minutes at an intensity of (35-65%) of maximum heart rate؛ and supplementation group supplemented with celery seed supplement 3 times daily after meal; and after eight weeks of exercise Blood samples were taken 24 hours before the study and 48 hours after the last training session. Data were analyzed using SPSS software. Celery seed supplementation and Pilates training had no significant decrease on rheumatoid arthritis. The results of the present study showed that eight weeks of Pilates training with celery seed supplement led to decline (ANA) in middle-aged women with rheumatoid arthritis.
Prescriptive formulation of Inorganic Membranes on Aqueous Surface
Authors: Ho Ghim Wei , Zhang Chen , Lu Wanheng , Xu Yingfeng , Zeng K. Y.
Published: June 2022

Freestanding functional inorganic membranes have piqued immense interest, as they may extend the notion of a selective barrier beyond matter flow to encompass energy and even information flows, potentially unlocking new possibilities in advanced separation, catalysis, sensors, memories, optical filtering and ionic conductors. However, limited by the brittle nature of most inorganic materials along with their few surface unsaturated linkages, inorganic membranes are far less ubiquitous than their organic counterparts, which may be easily obtained from diverse top-down moldings and/or bottom-up syntheses. Up to now, only a few specific inorganic membranes were circuitously derived from the pre-deposited films by selective removal of the sacrificial substrates. Here, we demonstrate a facile strategy to switch the nucleation preference in the aqueous system, resulting in a versatile synthesis of various ultrathin inorganic membranes on solution surface. Through a comprehensive understanding of the kinematic evolution of floating building units for membrane construction, the phase diagram based on geometrical connectivity, as well as the principle of customizing membrane thickness and porosity, are established, providing access to unusual membrane topologies with complex architectures. This universal synthetic methodology, pivotal to both scientific and industrial communities, will hasten the exploitation of new functional membranes for a variety of novel applications.
Complete chloroplast genome sequences of Gynostemma guangxiense: genome structure, codon usage bias and phylogenetic relationships in Gynostemma (Cucurbitaceae)
Authors: Zhao Yuemei , Zhang Xiao , Zhou Tao , Chen Xiaodan , Ding Bo
Published: June 2022

Gynostemma guangxiense is an important medicinal species distributed in Guangxi, China. In this study, we not only obtained the complete chloroplast (cp) genome sequence of G. Guangxiense by Illumina paired-end sequencing technology, but also analyzed its codon usage pattern using bioinformatics approaches. The cp genome of G. guangxiense was 157,785 bp with a pair of inverted repeat (IR) regions (26,288 bp) separated by one large single copy (LSC) region (86,702 bp) and one small single copy (SSC) region (18,507 bp). The whole genome contains 130 unique genes, of which 113 are unique, including 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. In addition, 62 repeats and 70 simple sequence repeats (SSRs) were identified, phylogenetic inference based on 73 protein coding genes indicated that G. guangxiense had the closest relationship to G. caulopterum. 52 CDSs longer than 300 bp in G. guangxiense cp genome were screened to analyze synonymous codon usage. The neutrality plot reflected a weak correlation between GC12 and GC3; ENC plot analysis showed that a majority of genes distributed below the expected curve; PR2-plot mapping analysis revealed G and T were used more frequently than C and A at the third base position. Finally, a total of 16 codons were identified as the optimal codons. These findings suggested that selection was the major pressure to influence the codon usage in G. guangxiense cp genomes. Our study provided an important theoretical basis for molecular identification, utilization and conservation in G. guangxiense.
The transcription factor HIF2α partakes in the differentiation block of acute myeloid leukemia
Authors: Magliulo Daniela , Caserta Carolina , Belluschi Serena , Fracassi Cristina , Giannetti Kety , Pini Raffaella , Zapparoli Ettore , Beretta Stefano , Ugge' Martina , Simoni Matilde , Draghi Eleonora , Coltella Nadia , Tresoldi Cristina , Morelli Marco , Micco Raffaella Di , Gentner Bernhard , Vago Luca , Bernardi Rosa
Published: June 2022

Differentiation arrest along the myeloid lineage is a defining feature of acute myeloid leukemia (AML), but its molecular determinants remain poorly defined. Pharmacological removal of the differentiation block leads to leukemia eradication in acute promyelocytic leukemia (APL) patients but has not yet been successfully translated to non-APL AMLs. Here, by investigating the function of hypoxia-inducible transcription factors HIF1α and HIF2α, we identify HIF2α as a new regulator of the AML differentiation block. Mechanistically, HIF2α cooperates with EZH2, the catalytic subunit of polycomb repressive complex 2, to promote deposition of the repressive H3K27me3 histone mark at the regulatory regions of myeloid differentiation genes. Thus, inhibiting HIF2α releases an epigenetic break to leukemic blasts maturation and cooperates with the pro-differentiation agent all-trans retinoic acid to trigger AML differentiation. We propose that HIF2α inhibition may open new therapeutic avenues for AML treatment by licensing blasts maturation and forcing leukemia debulking.
Temperature-dependent development of Agrotis ipsilon (Lepidoptera: Noctuidae) and its stage transition models
Authors: Lee Young Su , Lee Hee-A , Kim Gil-Hah , Kim Soo-Bin , Kim Dong-Soon
Published: June 2022

The black cut worm, Agrotis ipsilon (Hufnagel), is a destructive crop pest worldwide in tropical and subtropical regions, and a typical pest damaging plant parts below the soil surface, which requires thorough prediction of target stage for proper managing. This study was conducted to evaluate thermal effect on the development of A. ipsilon using Kimchi cabbage (Brassica campestris) as larval diet in a wide range of temperatures (10 to 40 ℃) in the laboratory. Temperature significantly affected the development time of eggs, larvae, and pupae of A. ipsilon. Using a linear model between temperature and development rate (1/days), lower threshold temperatures (LT) for eggs, larvae, and pupae were estimated to be 12.1 ℃, 9.6 ℃, and 11.2℃, respectively. Thermal constants (degree days, DD) for eggs, larvae, and pupae stages were 31.3 DD, 342.2 DD, and 181.3 DD, respectively. Thermal constant for each larval instar was also determined suing a common LT of 10.4 ℃: 40.3 DD for eggs, 315.6 DD for total larvae, and 199.6 DD for pupae. Stage transition models for all stages were constructed using two basic components of nonlinear development rate and distribution models to simulate the proportion of individuals shifted from one stage to the next stage. These models will be useful for constructing a population model for A. ipsilon in the future. Furthermore, the variation of development time of A. ipsilon reported in previous studies was discussed using 95% confidence limits of the estimated line of our nonlinear models.
Clinical anatomy of coronoid foramina of mandible and review of its implications in maxillofacial surgery
Authors: S Nyer Firdoose C , AlSaleh Mohamad Mahmoud Awadalla , S Ghousia , Khair Syeda Umul
Published: June 2022

A foramen in the coronoid process of the human mandible is a recently identified anatomic variant. This anatomical-radiological study aims to explore the variant foramina using cone beam computed tomography while determining its prevalence and evaluating its variability in terms of unilateral, bilateral, or gender predilection and propose the significance of this unique anatomic finding. Mandibular cone beam computed tomography scans from a total of 2,168 patients were evaluated on a prospective and retrospective scale for confirmation of the coronoid foramen after first identification over a panoramic radiograph. The coronoid foramen was found and confirmed in 96 (4.4%) patients. This script identifying the coronoid foramina of mandible becomes requisite research as a foundation for further analysis due to the scarcity of literature on this recent find.
On-Line Monitoring of Oxidation States in a HSS Work Roll During Hot Rolling Using Pulsed Eddy Current Testing
Authors: Wei Chen , Song Shouxu , Li Xiang , Tian Yongting
Published: June 2022

The quality of rolled products and consumption of rolls are greatly influenced by the oxidation behavior of work roll surface in hot rolling. It is of great significance to improve the quality of rolled products and reduce the consumption of rolls to monitor and evaluate the oxidation states of work rolls in service by non-contact nondestructive inspection technology. In this study, a self-developed test apparatus simulated the high-speed steel (HSS) work roll oxidation in real hot rolling conditions. The interaction mechanism between oxidation and wear of HSS work roll under different conditions was revealed. Furthermore, a set of pulsed eddy current (PEC) nondestructive inspection system was designed and built to evaluate different oxidation states of HSS work roll in service. Variational mode decomposition (VMD) Hilbert transformation was proposed to overcome the problem of mode mixing in mode decomposition of oxidation monitoring signals. At last, the Hilbert marginal spectrum centroid was presented as a characteristic for quantifying the surface oxidation state of work rolls in service.
Research on laparoscopic surgical instrument detection technology based on multi-attention enhanced feature pyramid network
Authors: Wang Xinying , Zhang Yuxuan , Li Yang
Published: June 2022

Laparoscopic surgical instruments are difficult in object detection due to their large-scale changes. Feature Pyramid Network (FPN) can effectively solve the multi-scale object detection problem. However, there are still some problems in FPN that limit the full utilization of multi-scale features. By analyzing the design problem of FPN, we propose the Multi-Attention Enhanced Feature Pyramid Network (MAFPN). First, we replace the convolutional block with feature selection module (FSM) that combines channel attention and global attention, which selectively maintains important information and enhances the expressiveness of features at each scale. Second, the global contextual information is captured by the self-attentive augmented fusion module (AAFM), which enriches the high-level feature information in the FPN and enhances the feature fusion effect. Finally, we use Dynamic Convolution Decomposition (DCD) to alleviate the impact of upsampling while enhancing the feature expression ability. Experimental results on the laparoscopic surgical instrument detection dataset m2cai16-tools-locations indicate that when ResNet50 is used as the backbone network, MAFPN improves the baseline network by 1.8 percentage points, to 96.5AP.
Spatial dysregulation of T follicular helper cells impairs vaccine responses in ageing
Authors: Linterman Michelle , Silva-Cayetano Alyssa , Fra-Bido Sigrid , Innocentin Silvia , Lee Jia Le , Webb Louise , Foster William , Burton Alice , Bignon Alexandre , Vanderleyden Ine , Carr Edward , Hill Danika , Denton Alice , Robert Philippe , Meyer-Hermann Michael , Alterauge Dominik , Baumjohann Dirk , Lemos Julia , Espeli Marion , Balabanian Karl
Published: June 2022

Vaccination generates long-lived antibody-mediated immunity against (re-)infection. This humoral immunity is derived from memory B cells and long-lived antibody-secreting plasma cells via the germinal centre (GC) response. The magnitude and quality of the GC response declines with age, resulting in poor vaccine-induced immunity in older individuals, but the causal factor/s of this age-related decline are unknown. A functional GC requires the co-ordination of multiple cell types across time and space, in particular across its two functionally distinct compartments: the light and dark zones. We identified that the spatial organisation of the GC is altered in ageing, with CXCR4-mediated mislocalisation of T follicular helper (Tfh) cells in the dark zone and a compressed network of follicular dendritic cells (FDCs) in the light zone. By modulating the positioning of Tfh cells in vivo, we found that Tfh cell polarisation is critical for the quality of the antibody response, and, surprisingly, for the expansion of the FDC network upon immunisation. The smaller GC responses and defective FDC expansion in ageing were corrected by provision of Tfh cells that co-localise with FDCs via CXCR5:CXCL13-mediated interaction. This demonstrates that the age-dependent defects in the GC response are reversible and shows that Tfh cells have a dual role in B cell help and facilitating stromal cell responses to vaccines.
Designing of multiple diagnostic primers for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Omicron variant (B.1.1.529/BA)
Authors: Gupta Amit Kumar , Kumar Manoj
Published: June 2022

The world is facing the SARS-CoV-2 pandemic and COVID-19 disease with more than 63 lakh mortalities as of 7th June 2022. Multiple variants and lineages of SARS-CoV-2 were evolved. Recently on 24th November 2021, a new variant of SARS-CoV-2, named Omicron (B.1.1.529/BA, Pango lineage) was reported with a high number of mutations on spike protein and further classified as a variant of concern. It is very critical to detect and diagnose variants timely and effectively. We have designed multiple sets of diagnostic primers and probes specific for the Omicron variant utilizing the spike region. In total, we are providing 24 sets of diagnostic primers for the Real-Time RT-PCR with associated probes. Further, 32 sets of primers were also provided for PCR assays. For all the primers, forward-reverse sequences, start-end, Tm, and product length are also provided. These could be utilized and tested for omicron detection. Similarly, we also provide experimentally employed primer/probe data sets for RT-PCR and nested RT-PCR of omicron variants. We anticipate this effort would be useful for the scientific community and fulfill a much-needed requirement.
Tracing environmental changes according to the spatiotemporal variability of the hydrogeochemical background of springs: a case of Lithuania
Authors: Slavinskienė Gintarė , Satkūnas Jonas , Taminskas Julius , Linkevičienė Rita , Baubinienė Aldona , Arustienė Jurga
Published: June 2022

The qualitative status of groundwater is often underestimated. This is because distinguishing trends of hydrogeochemistry variation linked to anthropogenic pressure from natural trends in groundwater is challenging. The study aims to evaluate the effect of the natural processes on the limits of temporal variability of the natural background level (NBL) in springs draining aquifer units with different vulnerabilities. Shallow groundwater samples of eighteen natural springs were investigated. Statistical analysis and geochemical modelling were used. The results showed that the natural temporal variability limits of HCO3−, Na+, K+, Cl− and SO42− in high vulnerability aquifers were narrower than in moderate and low vulnerability aquifers. This pattern was determined by the stronger buffering effect of carbonate equilibrium, sulphate reduction, and dilution processes on the variation of these major ions in high vulnerability aquifers. Meanwhile, natural temporal variability limits of TDS, Ca2+ and Mg2+ in high vulnerability aquifers were wider than in moderate and low vulnerability aquifers. This pattern was related to the stronger buffering effect of ion exchange on the variability limits of Ca2+ and Mg2+ in springs of moderate and low vulnerability aquifer units than the buffering effect of weaker carbonate dissolution and higher dilution intensity on the variability limits of Ca2+ and Mg2+ in springs of high vulnerability aquifer. The results of this research could be helpful for the reasonable integrated management and effective protection of groundwater resources.
Long-term radiographic and clinical outcomes in patients undergoing transforaminal endoscopic lumbar discectomy: a propensity score matching study
Authors: Mu Guanzhang , Yue Lei , Sun Haolin
Published: June 2022

Background
Percutaneous Endoscopic Transforaminal Discectomy (PETD) is an evolving technique for spinal disorders. But what comes along with the widespread use of PETD are treatment related side-effects. The evidence regarding the long-term effects of PETD is needed. Our study aimed to fill the knowledge gap of long-term clinical and radiographic effects of PETD on lumbar disc herniation.
Methods
Radiographic and clinical data of patients undergoing TELD in our institution from January 2015 to January 2019 were retrospectively collected. LDH outpatients who had not received surgical treatment during the same period were 1:1 matched by propensity score matching as the conservative group. The radiographic parameters of the two groups at baseline and at the last follow-up (≥ 24 months) were analyzed. For the TELD group, patient-reported clinical outcomes and the incidence of reoperation were analyzed.
Results
A total of 47 patients in the TELD group were included in this study, and 47 patients in the conservative group were matched by propensity score matching. The disc height of the TELD group at the last follow-up was lower than that at the baseline (P < 0.001), and lower than that of the conservative group at the last follow-up (P < 0.05). The disc degeneration grade of the TELD group at the last follow-up was greater than that at the baseline, and greater than that of the conservative group at the last follow-up. There was no significant difference in the facet joint degeneration in the TELD group between the baseline and the last follow-up, and between the TELD group and the conservative group at the last follow-up (P > 0.05). The pain intensity and disability score in the TELD group at 3-month follow-up and at the last follow-up were significantly lower than those at the baseline (P < 0.001). Six patients in the TELD group required additional surgery during the follow-up period.
Conclusion
The disc height of the operated level was significantly reduced and the disc degeneration was significantly aggravated in TELD-treated patients at the long-term follow-up; in contrast, the facet joint degeneration did not show significant aggravation. Despite the satisfying therapeutic results, patients undergoing TELD should still be informed of the risk of reoperation.
Immunization with rEmCRT provides protection against Echinococcus multilocularis infection in BALB/c mice
Authors: Chen Lujuan , Cheng Zhe , Xian Siqi , Xu Zhijian , Yan Yan , Chen Jianfang , Wang Yanhai , Zhao Limei
Published: June 2022

Background
Alveolar echinococcosis (AE) is a severe parasitic zoonosis, caused by the larval stage of Echinococcus multilocularis. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Echinococcus infection.
Methods
First of all, we predicted the physical and chemical properties and protein structure characteristics of E. multilocularis calreticulin (EmCRT), and then constructed the recombinant plasmid pET28a-EmCRT. Secondly, the constructed recombinant plasmid was transformed into E. coli BL21 and induced to produce recombinant EmCRT (rEmCRT). Then the antigen specificity and the calcium-binding property of rEmCRT was detected. Thirdly, the expression level and distribution of Emcrt gene in PSCs and vesicles were analysed. And the protective immunity induced by rEmCRT immunized mice and the corresponding mechanismwas investigated in the end.
Results
We identified that EmCRT, a ubiquitous protein with Ca2+-binding ability, elicited protective immunity against E. multilocularis infection. EmCRT was found to be expressed on the surface of E. multilocularis larvae as well as in the secreted products of metacestode vesicles and protoscoleces (PSCs). Recombinant EmCRT formulated with Freund’s adjuvant (FA) elicited both IgG1 and IgG2a antibodies, with a higher IgG1/IgG2a ratio indicating a predominant Th2 response in vaccinated mice, and stimulated mouse lymphocytes from the spleen to produce high levels of Th1 (IFN-γ, IL-2) and Th2 (IL-4, 5, 10) cytokines. The protection was around 43.7% after challenged with E. multilocularis PSCs.
Conclusions
Our results suggest that EmCRT is an immunodominant protein secreted by E. multilocularis larvae and contributes to the induction of partial protective immunity in vaccinated mice against Echinococcus infection. Therefore, EmCRT could be a novel and potential candidate vaccine against AE.
Is the Histologic Finding of Smoking-Related Interstitial Fibrosis (SRIF) Specific for Smoking? Histologic Analysis of 600 Surgical Lung Specimens from Current Smokers, Ex-smokers and Never-Smokers
Authors: Stegenga Andrea , Mukhopadhyay Sanjay
Published: June 2022

Respiratory bronchiolitis (RB) and emphysema are histologic manifestations of cigarette smoking in lung parenchyma. In 2010, a histologic finding known as smoking-related interstitial fibrosis (SRIF) was described in smokers. We undertook this study to determine the specificity of SRIF as a histologic marker of smoking. An H&E-stained slide representative of non-neoplastic lung was selected from 600 consecutive surgically resected lung specimens and assessed for RB, emphysema and SRIF by a pulmonary pathologist blinded to smoking status. Smoking status was retrieved from medical records by another pathologist. The specificity of each histologic finding for smoking status was calculated. There were 600 lungs from 600 patients (309 men, 291 women; mean age 62y, range 6–91) including 444 smokers (88 current, 356 ex) and 156 never-smokers. Sixty-eight cases with SRIF (68/600, 11%) were diagnosed by the pathologist blinded to smoking status; in all 68, the patient was a smoker (current smokers 25; ex-smokers 43; 34 men/34 women, mean age 63y, range 33-82y) with a mean of 46.7 pack-years of smoking (range 7.5-122.5 pack years). Of 139 cases with RB alone, 98 were smokers and 41 were never-smokers. Of 43 patients with emphysema alone, 34 were smokers and 9 were never-smokers. Of 197 cases with RB plus emphysema, 173 were smokers and 24 were never-smokers. The specificity of SRIF for smokers (100%) was higher than RB alone (74%), emphysema alone (94%), and RB plus emphysema (85%). SRIF occurs exclusively in cigarette smokers, and is a more specific smoking-related histologic finding than RB or emphysema.
Interleukins and the risk of non-alcoholic fatty liver disease: a two-sample Mendelian randomization study
Authors: Wang Fang , Mi Jiarui , Bai Xiaoyin , Ruan Gechong , Zhang Yu , Yu Kang
Published: June 2022

Background and AimsInterleukins (ILs) are associated with non-alcoholic fatty liver disease (NAFLD), however the direction and causal effects of them susceptibility remain unclear. Two-sample Mendelian randomization (MR) study was performed to investigate the causal relationship.Materials and MethodsSingle nucleotide polymorphisms associated with 10 ILs were used as instrumental variables. Summary-level results of estimates for the associations of NAFLD were obtained from two large multi-centered NAFLD genome-wide association study (2,377 cases and 23,5679 controls). For univariable MR, inverse-variance weighted (IVW) method was applied, while MR-Egger, Weighted Median and MRPRESSO were conducted as sensitivity analyses. Lastly, we performed meta-analyses with estimates from the two cohorts.ResultsIL-1 receptor antagonist (IL-1Ra) demonstrated a suggested significant association with an increased risk of NAFLD in both cohorts, with OR 1.435 (95%CI, 1.041–1.977, P = 0.0270) and 1.424 (95%CI, 1.011–2.004, P = 0.0430), respectively. The combined estimate OR 1.430 (95%CI, 1.132–1.807, P = 0.0027). Furthermore, genetically proxied IL-6 and IL-6 receptor alpha (IL-6Ra) levels displayed consistent positive trend with an increased risk of NAFLD, although none of them reach suggestive significance. Notably, the pooled estimates were 1.827 (95% CI, 1.328–2.514, P = 0.0002) for IL-6 and 1.098 (95% CI, 1.026–1.176, P = 0.0068) for IL-6Ra, respectively. The sensitivity analysis showed consistent positive trend towards the increased NAFLD susceptibility, though none of them achieved statistical significance.ConclusionThe results suggested genetically proxied circulating of IL-1Ra, IL-6, and IL-6Ra are causally associated with the risk of NAFLD. Moreover, our data highlights the potential targeted measures and drug repurposing strategies in the NAFLD prevention and treatment.