bluuebunny/crossref_metadata_2025 huggingface dataset:
Do Naturalistic Developmental Behavioral Interventions improve family quality of life? A systematic review and meta-analysis
Authors: Duncan Amanda Leigh , Keene Hannah , Shepley Collin
Published: February 2024

Naturalistic Developmental Behavioral Interventions have been described as culturally responsive and family-friendly interventions, with research demonstrating improvements in children’s development when receiving these services. Given the child-directed nature of Naturalistic Developmental Behavioral Interventions and the intervention’s congruence with families’ daily routines, many studies have examined the impact of Naturalistic Developmental Behavioral Interventions on family and family member quality of life. Therefore, we conducted a systematic review and meta-analysis to explore the relationship between Naturalistic Developmental Behavioral Interventions and family quality of life. Results suggest that the receipt of Naturalistic Developmental Behavioral Intervention services neither improved nor worsened family and family member quality of life. For those involved in the provision of Naturalistic Developmental Behavioral Intervention services, there is an immediate need to convey to families that children’s improvements will likely not translate into improvements in family quality of life (osf.io/3ua5f).

Lay abstract

Naturalistic Developmental Behavioral Interventions have been described as culturally responsive and family-friendly interventions, with research demonstrating improvements in children’s development following the receipt of these interventions. Given the child-directed nature of Naturalistic Developmental Behavioral Interventions and the intervention’s integration within families’ daily routines, many studies have examined the impact of Naturalistic Developmental Behavioral Interventions on family and family member quality of life. We conducted a systematic review and meta-analysis to explore the relationship between Naturalistic Developmental Behavioral Interventions and family quality of life. Results suggest that the provision of a Naturalistic Developmental Behavioral Intervention neither improved nor worsened family or family member quality of life. For those involved in delivering Naturalistic Developmental Behavioral Intervention services, there is an immediate need to convey to families that children’s improvements will likely not translate into improvements in family quality of life.
Characterizing research partnerships in child health research: A scoping review
Authors: Crockett Leah K , Scott Shannon D , Driedger S Michelle , Khan Masood , Prabhu Devashree , Askin Nicole , Steliga Dawn , Tefft Olivia , Jansson Ann , Turner Sarah , Sibley Kathryn M
Published: February 2024

Research partnerships between researchers and knowledge users (KUs) in child health are understudied. This study examined the scope of KU engagement reported in published child health research, inclusive of health research partnership approaches and KU groups. Search strategies were developed by a health research librarian. Studies had to be in English, published since 2007, and were not excluded based on design. A two-step, multiple-person hybrid screening approach was used for study inclusion. Data on study and engagement characteristics, barriers and facilitators, and effects were extracted by one reviewer, with 10% verified by a second reviewer. Three hundred fifteen articles were included, with 243 (77.1%) published between 2019 and 2021. Community-based participatory research was the most common approach used ( n = 122, 38.3%). Most studies ( n = 235, 74.6%) engaged multiple KU groups (range 1–11), with children/youth, healthcare professionals, and parents/families being most frequently engaged. Reporting of barriers and facilitators and effects were variable, reported in 170 (53.8%) and 197 (62.5%) studies, respectively. Publications have increased exponentially over time. There is ongoing need to optimize evaluation and reporting consistency to facilitate growth in the field. Additional studies are needed to further our understanding of research partnerships in child health.
Shifting narratives: A critical discourse analysis of racial bias in the tale of Wu Feng’s indigenous representation
Authors: Chu Rong-Xuan
Published: February 2024

Heroic tales can support a nation’s mainstream ideology, but for minority groups, such as indigenous peoples, they can also be a detrimental oppositional force. This study explores the evolving discourse surrounding the tale of Wu Feng, arguably the most widespread and prominent narrative responsible for traumatizing indigenous peoples in Taiwan. Employing a critical discourse analysis of three texts from significant historical eras (Taiwan under Japanese rule, the martial-law era under the Nationalist Kuomintang’s rule, and the beginning of Taiwan’s indigenous rights movements), the study reveals the shifting portrayal of Taiwan’s indigenous peoples over time. Additionally, by incorporating data obtained by interviewing key stakeholders, it discusses the enduring ramifications of the tale for Taiwan’s indigenous communities. In conclusion, this paper urges that increased attention be directed toward recognizing the voices and experiences of minorities as represented in historical tales, to ensure greater inclusivity and balance.
Operationalizing Street Harassment Using Survey Instruments: A Systematic Review of Measuring Harassment in Public Spaces Using Surveys
Authors: Keel Chloe , Stewart Rebecca , Mellberg Jacques
Published: February 2024

Quantitative research has an omitted variable problem when it comes to measuring and modeling non-criminal threats in the urban environment. This systematic review identified questionnaires and surveys used to measure incidences of street harassment/harassment in public, to discuss how they operationalize street harassment/harassment in public, and to report the characteristics of those with the best evidence of reliability and validity. We searched five databases and included peer-reviewed articles published in English from 1994 to 2022 that measured street harassment using a survey instrument. Our search resulted in 54 included studies. Of these studies, 16 primarily focused on understanding street harassment. To design effective prevention strategies in response to street harassment, research must first effectively measure the prevalence of street harassment and the contexts in which street harassment occurs. Due to the inconsistencies in definition, our review identified prevalence rates were inconsistent. Incidents of street harassment provide a promising avenue for future research, although scholars must first seek to appropriately operationalize this concept in survey research. We provide suggestions for future research that seeks to use surveys to understand harassment in public places.
Understanding Newcomer Challenges and Opportunities to Accessing Nature and Greenspace in Riverdale, Hamilton, Ontario: A Neighborhood-Centered Photovoice Study
Authors: Kandasamy Sujane , Kwan Matthew Y. , Memon Parsa K. , Desai Dipika , de Souza Russell J. , Montague Patty , Sherifali Diana , Wahi Gita , Anand Sonia
Published: February 2024

Background

Access to and engagement with greenspace is related to improved health benefits. We sought to collaborate with community members as partners in research and co-creators in knowledge to better understand which components within a newcomer-dense community help or hinder individual and community efforts to access greenspace and nature-based activities.

Methods

We used photovoice methodology to engage with local residents in focus groups, photowalks, and photo-elicitation interviews. Themes were developed using direct content analysis.

Results

A total of 39 participants (ages 11–70 years; median years in Canada of 3.25 years) were engaged in this program of research. From the analysis, we developed four themes: (a) peace and beauty; (b) memories of home; (c) safety and cleanliness; and (d) welcoming strengthened and new opportunities. Participants associated nature with peace, citing it as “under-rated” but “vital” to the neighborhood. Via photographs and stories, participants also shared a multitude of safety concerns that prevent their access to green/outdoor spaces for healthy active living programs or activities (e.g., woodchip-covered playgrounds, ample amounts of garbage littering the park and school grounds, lack of timely ice removal on sidewalks, limited safe biking paths, and unsafe motor vehicle practices at the crosswalks surrounding local parks).

Conclusion

To translate the key ideas and themes into an informed discussion with policy and decision-makers, we held an in-person exhibition and guided tour where community members, the lead photovoice researcher, and SCORE! principal investigator shared information about each theme in the form of a pseudo-narrative peppered with prepared discussion questions.
School Engagement as a Mediator of the Relationship Between Temperament and Recidivism Among Adolescents in Community-Based Juvenile Justice Services
Authors: Lang Joshua A. , Wolff Kevin T. , Baglivio Michael T.
Published: February 2024

Prior research has established the relationship between temperament and recidivism, yet the mechanisms that underlie this relationship have yet to be explored. School engagement represents one possible mechanism and has been shown to be related to both recidivism and temperament. The current study expands research on DeLisi and Vaughn’s temperament theory by utilizing data from the Florida Department of Juvenile Justice to determine if school engagement effectively mediates the relationship between temperament and continued delinquency. Results provide evidence of a significant mediation effect, controlling for a host of potential confounding variables. Findings suggest that approximately one-third of the total effect of temperament on juvenile recidivism is mediated by school engagement issues, yet a significant direct effect of temperament remains. Policy and intervention implications are briefly considered. Further research is required to determine additional mediators to better understand the processes by which temperament impacts reoffending.
Associations of Circadian Rest-Activity Rhythms With Affect and Cognition in Community-Dwelling Stroke Survivors: An Ambulatory Assessment Study
Authors: Lau Stephen C. L. , Connor Lisa Tabor , Skidmore Elizabeth R.
Published: February 2024

Background

Rest-activity rhythm (RAR) is a modifiable behavioral factor associated with affect and cognition. Identifying RAR characteristics associated with affect and cognition among stroke survivors provides insight into preventing poststroke affective and cognitive impairment.

Objective

To examine the associations of RAR characteristics with affect and cognition among community-dwelling stroke survivors.

Methods

Forty participants with mild stroke (mean age = 52.8; 42.5% female; 55% White) reported their affect and cognitive complaints using ecological momentary assessment and wore an accelerometer for 7 consecutive days and completed the National Institutes of Health Toolbox Cognition Battery. RAR characteristics were extracted using parametric and non-parametric approaches. Multivariable linear regressions were used to identify RAR characteristics associated with affect and cognition.

Results

Later onset of rest ( B = 0.45; P = .008) and activity ( B = 0.36; P = .041) were positively associated with depressed affect. These associations were reversed for cheerful effect (rest onset: B = −0.42; P = .017; activity onset: B = −0.39; P = .033). Cheerful affect was also positively associated with relative amplitude (ie, distinctions in activity levels between rest and activity; B = .39; P = .030). Intra-daily variability (ie, RAR fragmentation; B = 0.35; P = .042) and later onset of activity ( B = .36; P = .048) were positively associated with cognitive complaints. Less erratic RAR was positively associated with fluid cognition ( B = 0.29; P = .036); RAR fragmentation was positively associated with crystallized cognition ( B = 0.39; P = .015).

Conclusions

We identified RAR correlates of affect and cognition among stroke survivors, highlighting the value of managing RAR and sleep in stroke rehabilitation. Future studies should test whether advancing the onset of rest and activity, promoting a regular active lifestyle, and improving rest and sleep in the nighttime protect stroke survivors from affective and cognitive impairment.
Using Ethno-Epidemiology in a Prospective Observational Study to Increase the Rigour of Nested Qualitative Research
Authors: S Walker , P Dietze , P Higgs , K Rathnayake , T Kerr , B Ward , L Maher
Published: February 2024

Ethnographic-epidemiological (“ethno-epi”) research methodologies are increasingly being used to examine health-related issues, including the experiences of people who use drugs. However, the complementary application of random sampling from a well characterised cohort and qualitative data collection methods in a single study has not been described. We address this gap by sharing insights from the implementation of a novel random stratified sampling technique to recruit participants from two large prospective observational studies of people who use drugs into a qualitative study about impacts of the COVID-19 pandemic on their lived experience. We aim to describe how an ethno-epi approach we used can enhance the validity, reliability and generalizability of research findings in mixed methods investigations. We do so by providing a step-by-step description of the process we used to determine participant eligibility and recruitment into the qualitative study. Although the approach is not without limitations, findings underscore how ethno-epi random sampling approaches can increase the credibility and trustworthiness of qualitative findings without compromising data depth and integrity. Our study makes an important contribution to the growing number of new creative approaches being developed in the mixed methods research field and we hope that by sharing our account it will encourage and support others to consider the use of ethno-epi approaches in health-related research.
COVID-19 and figures of blame: Discursive representations of blame for COVID-19 and its impacts in UK online news
Authors: Matthews Jamie , Heesambee Farzeen
Published: February 2024

As publics have attempted to make sense of the COVID-19 crisis and its longer-term impacts there has been an inevitable search for blame. Emergent research on the attribution of blame has focussed exclusively on the initial outbreak, with insufficient attention paid to how countries have responded to the pandemic. Our study adopts a longitudinal approach, examining the figures of blame that emerged across the UK’s experience of COVID-19, including subsequent waves of COVID-19. By sampling articles from three online UK news outlets (BBC; The Guardian; Mail Online), we analyse the linguistic elements and discourse strategies that contribute to the representation of specific actors as figures of blame in news coverage of COVID-19. To identify actors and their representations we focus on three elements: (1) direct, indirect or implied reference to an actor; (2) an expression of anger, resentment or frustration towards this actor; (3) textual and discursive features that nominate agency for their actions or inaction for a negative outcome. Our analysis shows that three prominent figures of blame emerged across the period of analysis. The primary actor represented as a figure of blame was the UK government. This, we argue, differs from the initial phases of the outbreak where there was an emphasis on externalising blame. We also found, however, that the public and the individual were constructed as figures of blame. For the latter it was through an emphasis on personal responsibility in the adoption of preventative behaviours and in following COVID-19 restrictions. We conclude the paper by exploring the significance of these findings for the communicative dynamics of the pandemic.
Logistics Service Quality and Product Satisfaction in E-Commerce
Authors: Rashid Dr. Aamir , Rasheed Dr. Rizwana
Published: February 2024

Consumer satisfaction in e-commerce directly depends on logistical services. There is a constant dilemma in which logistical service dimensions affect consumers’ product satisfaction. The aim is to determine how specific dimensions of logistics services affect product satisfaction in the e-commerce industry of Pakistan. For this research, a survey questionnaire with a deductive approach was utilized by collecting data from 197 respondents through a convenient sampling technique. Further, hypotheses were tested through a quantitative method with the help of SmartPLS. A measuring instrument was used with six dimensions, that is, availability, delivery time, shipping costs, product quality and condition, information quality, and customer satisfaction related to the product. This research revealed an insignificant effect of product availability, shipping time, and shipping cost on product satisfaction. In contrast, product quality and information quality were found to have a significant and positive effect on product satisfaction. From academic insights to logistical optimization, this research unlocks valuable knowledge for all stakeholders driving Pakistan’s e-commerce revolution. Buckle up for a journey into the heart of this rapidly evolving landscape.
Current Practices for Rehabilitation After Meniscus Repair: A Survey of Members of the American Orthopaedic Society for Sports Medicine
Authors: Cong Ting , Reddy Rajiv P. , Hall Arielle J. , Ernazarov Akhmad , Gladstone James
Published: February 2024

Background:

There is no consensus among sports medicine surgeons in North America on postoperative rehabilitation strategy after meniscus repair. Various meniscal tear types may necessitate a unique range of motion (ROM) and weightbearing rehabilitation protocol.

Purpose:

To assess the current landscape of how sports medicine practitioners in the American Orthopedic Society for Sports Medicine (AOSSM) rehabilitate patients after the repair of varying meniscal tears.

Study Design:

Cross-sectional study.

Methods:

A survey was distributed to 2973 AOSSM members by email. Participants reviewed arthroscopic images and brief patient history from 6 deidentified cases of meniscus repair—in cases 1 to 3, the tears retained hoop integrity (more stable repair), and in cases 4 to 6, the tear patterns represented a loss of hoop integrity. Cases were shuffled before the presentation. For each case, providers were asked at what postoperative time point they would permit (1) partial weightbearing (PWB), (2) full weightbearing (FWB), (3) full ROM, and (4) ROM allowed immediately after surgery.

Results:

In total, 451 surveys were completed (15.2% response). The times to PWB and FWB in cases 1 to 3 (median, 0 and 4 weeks, respectively) were significantly lower than those in cases 4 to 6 (median, 4 and 6 weeks, respectively) ( P < .001). In tears with retained hoop integrity, the median time to PWB was immediately after surgery, whereas in tears without hoop integrity, the median time to PWB was at 4 weeks postoperatively. Similarly, the median time to FWB in each tear with retained hoop integrity was 4 weeks after surgery, while it was 6 weeks in each tear without hoop integrity. However, regardless of tear type, most providers (67.1%) allowed 0° to 90° of ROM immediately after surgery and allowed full ROM at 6 weeks. Most providers (83.3%) braced the knee after repair regardless of hoop integrity and utilized synovial rasping/trephination with notch microfracture—a much lower proportion of providers utilized biologic augmentation (9%).

Conclusion:

Sports medicine practitioners in the AOSSM rehabilitated meniscal tears differently based on hoop integrity, with loss of hoop stresses triggering a more conservative approach. A majority braced and utilized in situ adjuncts for biological healing, while a minority added extrinsic biologics.
Selections From the ABC 2023 Annual Conference, Denver, Colorado, USA: Seeing the Future of Business Communication Teaching From a Mile High Perch
Authors: Whalen D. Joel , Drehmer Charles , Cavanaugh Andrew
Published: February 2024

Artificial intelligence assignments lead this article’s 11 teaching innovations selected from the My Favorite Assignments presented at the 2023 Association for Business Communication’s Annual International Conference held in Denver, Colorado. USA. Pedagogy presented here also includes ideas to enhance student engagement and techniques to transform learning via gamification.
Racialized Early Grade (Mis)Behavior: The Links Between Same-Race/Ethnicity Teachers and Discipline in Elementary School
Authors: Hwang NaYoung , Graff Patrick , Berends Mark
Published: February 2024

Studies persistently show disparities in exclusionary discipline across racial/ethnic groups in U.S. schools. Using administrative data from kindergarteners through fifth graders in Indiana, we examine the effects of student-teacher race/ethnicity matching on disciplinary outcomes. We find that Black students exhibit lower rates of suspension and expulsion when they study with Black teachers—driven mainly by fewer defiance and profanity offenses. By contrast, for Latinx and White students, having a teacher of the same race/ethnicity is not associated with suspension and expulsion. In light of the shortage of Black teachers in the teacher workforce, our findings underscore the vulnerability of Black students to exclusionary discipline in the early stages of schooling.
Cleaning Up the Neighborhood: White Influx and Differential Requests for Services
Authors: Dahir Nima , Hwang Jackelyn , Yu Ang
Published: February 2024

Visible signs of disorder serve as markers of difference across urban space. Sociological theory suggests that variation in collective social control efforts contributes to variation in physical disorder. However, how structural characteristics shape differences in informal social control remains underexplored because of limited data on disorder and social control. Using city service request data and a novel dataset drawing on Google Street View imagery and computer vision methods, the authors examine the neighborhood characteristics associated with propensities to request trash-related services across five large U.S. cities. The authors find that socioeconomically advantaged neighborhoods and those with fewer minority and foreign-born residents have higher propensities. However, an increase in White residents, but not necessarily an increase in high–socioeconomic status residents, is strongly associated with greater propensities. The authors argue that incoming White residents introduce unique dynamics of social control that are not necessarily collective, thereby affecting spatial inequality and power relations within their new neighborhoods.
The Protagonist of Value Creation in a Transformation Economy
Authors: Zhang Xueying
Published: February 2024

In today’s economy, consumers are increasingly motivated by emotions and self-realization, and have been described as ‘transformational aspirants’. These aspirants demand companies to understand their needs, co-create products/services/experiences with them and to provide transformational value. Therefore, this study aimed to identify the characteristics of ‘transformational aspirants’. A descriptive phenomenological approach was used, and semi-structured interviews were conducted for data collection. The results showed that ‘transformational aspirants’ have two major characteristics: otakufication and progression. This study contributes to the understanding and application of transformational value in business settings by clarifying the characteristics of transformational aspirants and demonstrating the importance of transformation for the promotion of sustainable customer relationships.
Glimpse of the Trials Presented at TCT and AHA 2023
Authors: Nagula Praveen , Parvathareddy Krishna Mala Konda Reddy
Published: February 2024

In this article, the late-breaking trials presented at TCT and AHA 2023 are briefly described. The trials are described under the headings according to the area of interest of the trial.
Transcriptomic analysis reveals the molecular basis of photoperiod-regulated sex differentiation in tropical pumpkins (Cucurbita moschata Duch.)
Authors: Xue Shudan , Huang Hexun , Xu Yingchao , Liu Ling , Meng Qitao , Zhu Jitong , Zhou Meijiang , Du Hu , Yao Chunpeng , Jin Qingmin , Nie Chengrong , Zhong Yujuan
Published: February 2024

Background

Photoperiod, or the length of the day, has a significant impact on the flowering and sex differentiation of photoperiod-sensitive crops. The “miben” pumpkin (the main type of Cucurbita moschata Duch.) is well-known for its high yield and strong disease resistance. However, its cultivation has been limited due to its sensitivity to photoperiod. This sensitivity imposes challenges on its widespread cultivation and may result in suboptimal yields in regions with specific daylength conditions. As a consequence, efforts are being made to explore potential strategies or breeding techniques to enhance its adaptability to a broader range of photoperiods, thus unlocking its full cultivation potential and further promoting its valuable traits in agriculture.

Results

This study aimed to identify photoperiod-insensitive germplasm exhibiting no difference in sex differentiation under different day-length conditions. The investigation involved a phenotypic analysis of photoperiod-sensitive (PPS) and photoperiod-insensitive (PPIS) pumpkin materials exposed to different day lengths, including long days (LDs) and short days (SDs). The results revealed that female flower differentiation was significantly inhibited in PPS_LD, while no differences were observed in the other three groups (PPS_SD, PPIS_LD, and PPIS_SD). Transcriptome analysis was carried out for these four groups to explore the main-effect genes of sex differentiation responsive to photoperiod. The main-effect gene subclusters were identified based on the principal component and hierarchical cluster analyses. Further, functional annotations and enrichment analysis revealed significant upregulation of photoreceptors (CmCRY1, F-box/kelch-repeat protein), circadian rhythm-related genes (CmGI, CmPRR9, etc.), and CONSTANS (CO) in PPS_LD. Conversely, a significant downregulation was observed in most Nuclear Factor Y (NF-Y) transcription factors. Regarding the gibberellic acid (GA) signal transduction pathway, positive regulators of GA signaling (CmSCL3, CmSCL13, and so forth) displayed higher expression levels, while the negative regulators of GA signaling, CmGAI, exhibited lower expression levels in PPS_LD. Notably, this effect was not observed in the synthetic pathway genes. Furthermore, genes associated with ethylene synthesis and signal transduction (CmACO3, CmACO1, CmERF118, CmERF118-like1,2, CmWIN1-like, and CmRAP2-7-like) showed significant downregulation.

Conclusions

This study offered a crucial theoretical and genetic basis for understanding how photoperiod influences the mechanism of female flower differentiation in pumpkins.
Colorectal cancer pre-diagnostic symptoms are associated with anatomic cancer site
Authors: Briggs Nicole L. , Ton Mimi , Malen Rachel C. , Reedy Adriana M. , Cohen Stacey A. , Phipps Amanda I. , Burnett-Hartman Andrea N. , Newcomb Polly A.
Published: February 2024

Background

Signs and red flag symptoms in colorectal cancer (CRC) patients who are below the recommended screening age are often overlooked, leading to delayed diagnosis and worse prognosis. This study investigates how patient pre-diagnostic symptoms are associated with anatomic site of their cancer and whether the association varies by age at CRC diagnosis.

Methods

We ascertained CRC patients’ experienced symptoms and screening through medical abstractions from an ongoing population-based study of CRC patients identified through a SEER cancer registry (N = 626). We used logistic regression to estimate odds ratios and 95% confidence intervals for the association between symptoms and CRC anatomic site. Additional analyses were stratified by age at diagnosis. Early-onset was defined as less than 50 years of age at CRC diagnosis.

Results

Participants who experienced blood in stool were more likely (odds ratio (95% confidence interval)) to have rectal (vs. colon) cancer (4.37 (3.02, 6.33)), as were patients who experienced changes to stool (1.78 (1.21, 2.60)). Patients diagnosed with colon cancer were more likely to present with abdominal pain (0.30 (0.19, 0.47)), anemia (0.40 (0.21, 0.75)), other symptoms (0.33 (0.19, 0.55)) and no symptoms (0.68 (0.44, 1.04)). When stratifying by age at diagnosis, we found that the association between blood in stool and rectal tumor location was particularly pronounced for patients with early-onset CRC (6.48 (2.73, 15.41)).

Conclusions

Common pre-diagnostic red flag symptoms are associated with CRC anatomic site. These findings can inform best practices for gastroenterologist triage of care and early evaluation of CRC and are of key importance given the rise of early-onset (pre-screening age) CRC.

Trial registration

Not applicable to this study and analysis.
Perioperative point-of-care-testing of plasmacholinesterases identifies older patients at risk for postoperative delirium: an observational prospective cohort study
Authors: Gruendel Matthias S. , Brenneisen Wibke , Wollborn Jakob , Haaker Gerrit , Meersch Melanie , Gurlit Simone , Goebel Ulrich
Published: February 2024

Background

Postoperative delirium (POD) is a severe perioperative complication that may increase mortality and length-of-stay in older patients. Moreover, POD is a major economic burden to any healthcare system. An altered expression of Acetylcholine- and Butyrylcholinesterases (AChE, BuChE) due to an unbalanced neuroinflammatory response to trauma or an operative stimulus has been reported to play an essential role in the development of POD. We investigated if perioperative measurement of cholinesterases (ChEs) can help identifying patients at risk for the occurrence of POD in both, scheduled and emergency surgery patients.

Methods

This monocentric prospective observational cohort study was performed in a tertiary hospital (departments of orthopaedic surgery and traumatology). One hundred and fifty-one patients aged above 75 years were enrolled for scheduled (n = 76) or trauma-related surgery (n = 75). Exclusion criteria were diagnosed dementia and anticholinergic medication. Plasma samples taken pre- and postoperatively were analysed regarding AChE and BuChE activity. Furthermore, perioperative assessment using different cognitive tests was performed. The type of anaesthesia (general vs. spinal anaesthesia) was analysed. Primary outcome was the incidence of POD assessed by the approved Confusion Assessment Method (CAM) in combination with the expression of AChE and BuChE.

Results

Of 151 patients included, 38 (25.2%) suffered from POD; 11 (14%) in scheduled and 27 (36%) in emergency patients. AChE levels showed no difference throughout groups or time course.

Trauma patients had lower BuChE levels prior to surgery than scheduled patients (p < 0.001). Decline in BuChE levels correlated positively with the incidence of POD (1669 vs. 1175 U/l; p < 0.001). Emergency patients with BuChE levels below 1556 U/L were at highest risk for POD. There were no differences regarding length of stay between groups or incidence of POD. The type of anaesthesia had no influence regarding the incidence of POD. Only Charlson Comorbidity Index and Mini Nutrition Assessment demonstrated reliable strength in respect of POD.

Conclusions

Perioperative measurement of BuChE activity can be used as a tool to identify patients at risk of POD. As a point-of-care test, quick results may alter the patients’ course prior to the development of POD.

Trial registration

https://drks.de/search/de/trial/DRKS00017178.
The global prevalence of depression, anxiety, and sleep disorder among patients coping with Post COVID-19 syndrome (long COVID): a systematic review and meta-analysis
Authors: Seighali Niloofar , Abdollahi Abolfazl , Shafiee Arman , Amini Mohammad Javad , Teymouri Athar Mohammad Mobin , Safari Omid , Faghfouri Parsa , Eskandari Alireza , Rostaii Omid , Salehi Amir Hossein , Soltani Hedieh , Hosseini Mahsa , Abhari Faeze Soltani , Maghsoudi Mohammad Reza , Jahanbakhshi Bahar , Bakhtiyari Mahmood
Published: February 2024

Background

Post COVID-19 syndrome, also known as "Long COVID," is a complex and multifaceted condition that affects individuals who have recovered from SARS-CoV-2 infection. This systematic review and meta-analysis aim to comprehensively assess the global prevalence of depression, anxiety, and sleep disorder in individuals coping with Post COVID-19 syndrome.

Methods

A rigorous search of electronic databases was conducted to identify original studies until 24 January 2023. The inclusion criteria comprised studies employing previously validated assessment tools for depression, anxiety, and sleep disorders, reporting prevalence rates, and encompassing patients of all age groups and geographical regions for subgroup analysis Random effects model was utilized for the meta-analysis. Meta-regression analysis was done.

Results

The pooled prevalence of depression and anxiety among patients coping with Post COVID-19 syndrome was estimated to be 23% (95% CI: 20%—26%; I2 = 99.9%) based on data from 143 studies with 7,782,124 participants and 132 studies with 9,320,687 participants, respectively. The pooled prevalence of sleep disorder among these patients, derived from 27 studies with 15,362 participants, was estimated to be 45% (95% CI: 37%—53%; I2 = 98.7%). Subgroup analyses based on geographical regions and assessment scales revealed significant variations in prevalence rates. Meta-regression analysis showed significant correlations between the prevalence and total sample size of studies, the age of participants, and the percentage of male participants. Publication bias was assessed using Doi plot visualization and the Peters test, revealing a potential source of publication bias for depression (p = 0.0085) and sleep disorder (p = 0.02). However, no evidence of publication bias was found for anxiety (p = 0.11).

Conclusion

This systematic review and meta-analysis demonstrate a considerable burden of mental health issues, including depression, anxiety, and sleep disorders, among individuals recovering from COVID-19. The findings emphasize the need for comprehensive mental health support and tailored interventions for patients experiencing persistent symptoms after COVID-19 recovery.
The Utrecht University Honours Program review project: example based scientific publishing training aimed at bachelor medical students
Authors: Bloothooft Meye , Pennings Helena J. M. , van der Heyden Marcel A. G.
Published: February 2024

Introduction

Medical undergraduate students receive limited education on scholarly publishing. However, publishing experiences during this phase are known to influence study and career paths. The medical bachelor Honours Program (HP) at Utrecht University initiated a hands-on writing and publishing course, which resulted in nine reviews published in internationally peer reviewed academic journals. We wanted to share the project set-up, explore the academic development of the participating students and determine the impact of the reviews on the scientific community.

Methods

Thirty-one out of 50 alumni completed a digital retrospective questionnaire on for example, development of skills and benefit for their studies and career. Publication metrics of the HP review papers were retrieved from Web of Science.

Results

This hands-on project provides a clear teaching method on academic writing and scholarly publishing in the bachelor medical curriculum. Participants were able to obtain and improve writing and publishing skills.

The output yielded well-recognized scientific papers and valuable learning experiences. 71% of the participating students published at least one additional paper following this project, and 55% of the students indicated the project influenced their academic study and/or career path. Nine manuscripts were published in journals with an average impact factor of 3.56 and cited on average 3.73 times per year.

Discussion

This course might inspire other medical educators to incorporate similar projects successfully into their curriculum. To this end, a number of recommendations with regard to supervision, time investment and group size are given.
Novel neutralizing SARS-CoV-2-specific mAbs offer detection of RBD linear epitopes
Authors: Zadeh Seyed Mostafa Mostafavi , Bayat Ali Ahmad , Shahsavarani Hosein , Karimi-Busheri Feridoun , Kiani Jafar , Ghods Roya , Madjd Zahra
Published: February 2024

Background

To stop the spread of the COVID-19 disease, it is crucial to create molecular tools to investigate and diagnose COVID-19. Current efforts focus on developing specific neutralizing monoclonal antibodies (NmAbs) elicited against the receptor-binding domain (RBD).

Methods

In the present study, recombinant RBD (rRBD) protein was produced in E. coli, followed by immunizing mice with purified rRBD. ELISA was applied to screen the hybridomas for positive reactivity with rRBD protein. The linear and conformational epitopes of the mAbs were subsequently identified using western blot. Finally, the reactivity, affinity, and neutralization activity of the purified mAbs were evaluated using ELISA.

Results

All mAbs exhibited similar reactivity trends towards both eukaryotic RBD and prokaryotic rRBD in ELISA. Among them, 2E7-D2 and 2B4-G8 mAbs demonstrated higher reactivity than other mAbs. Additionally, in western blot assays, these two mAbs could detect reducing and non-reducing rRBD, indicating recognition of linear epitopes. Notably, five mAbs effectively blocked rRBD- angiotensin-converting enzyme 2 (ACE2) interaction, while two high-affinity mAbs exhibited potent neutralizing activity against eukaryotic RBD.

Conclusion

In the current study, we generated and characterized new RBD-specific mAbs using the hybridoma technique that recognized linear and conformational epitopes in RBD with neutralization potency. Our mAbs are novel candidates for diagnosing and treating SARS-CoV-2.
Process evaluation of an integrated community-based intervention for promoting health equity in children in a new residential development area
Authors: Voss Stephan , Bauer Julia , Jung-Sievers Caroline , Moore Graham , Rehfuess Eva , Rhein Valerie Zu , Coenen Michaela
Published: February 2024

Background

Reducing health inequities for children from a disadvantaged background is an important task in public health. While intersectoral partnerships are a promising way to achieve this, few studies have examined the factors influencing the success of these interventions. In this study, we conducted a process evaluation of the integrated community-based intervention Präventionskette Freiham that the city of Munich, Germany, has implemented in a new residential development area. The aim was to investigate the implementation process as well as barriers and facilitators.

Methods

Following a mixed methods approach, we collected data from different core groups making up Präventionskette Freiham from April 2020 to August 2022, exploring their perspective on the implementation process. We conducted repeated qualitative interviews with the network coordinators and eleven local professionals from institutions engaged with or relevant for the intervention. We also undertook a focus group with four members of the advisory group representing the three municipal departments guiding the intervention. Ego-centered network maps were drawn by the network coordinators to chart the development of the network. Subsequently, we also conducted an online survey with local network members.

Results

At the early stage of the implementation process, the intervention was able to integrate actors from different sectors, serving as a platform for mutual exchange. However, the network produced limited output. According to the interviews, this may be mainly attributable to the early development status of the area. We identified seven topics that may act as facilitators or barriers to implementation of Präventionskette Freiham: (1) availability of resources, (2) political and administrative support, (3) the network coordinators, (4) network-internal processes, (5) trans-institutional cooperation, (6) perceived benefits of engagement, and (7) the output of the network.

Conclusions

The early development status of the area was a challenge for the intervention. This emphasizes the need to carefully consider context when planning and implementing integrated community-based public health interventions in new residential development areas.
TFR1 knockdown alleviates iron overload and mitochondrial dysfunction during neural differentiation of Alzheimer’s disease-derived induced pluripotent stem cells by interacting with GSK3B
Authors: Kang Tao , Han Zheng , Zhu Lijuan , Cao Bingqing
Published: February 2024

Iron metabolism disorders are implicated in the pathogenesis of Alzheimer’s disease (AD). It was previously reported that transferrin receptor (TFR1) expression was upregulated in AD mouse model. However, the precise biological functions of TFR1 in AD progression remains unclear. Herein, we observed a gradual increase in TFR1 protein expression during the differentiation of AD patient-derived induced pluripotent stem cells (AD-iPS). TFR1 knockdown inhibited the protein expression of ferritin and ferritin heavy chain 1 (FTH1), enhanced the expression of ferroportin 1 (FPN1), and decreased intracellular levels of total iron, labile iron, and reactive oxygen species (ROS). Moreover, TFR1 knockdown improved mitochondrial membrane potential (MMP), increased adenosine triphosphate (ATP) content, downregulated mitochondrial fission proteins, and upregulated mitochondrial fusion proteins. TFR1 knockdown alleviated iron overload and mitochondrial dysfunction in neural cells differentiated from AD-iPS, while TFR1 overexpression showed the opposite results. Additionally, TFR1interacted with glycogen synthase kinase 3 beta (GSK3B) and promoted GSK3B expression. GSK3B overexpression reversed the inhibitory effects of TFR1 knockdown on iron overload and mitochondrial dysfunction in AD-iPS differentiated neural cells. In conclusion, TFR1 knockdown alleviated iron overload and mitochondrial dysfunction in neural cells differentiated from AD-iPS by promoting GSK3B expression. Our findings provide a potential therapeutic target for the treatment of AD.

Graphical Abstract
A broad wastewater screening and clinical data surveillance for virus-related diseases in the metropolitan Detroit area in Michigan
Authors: Li Yabing , Miyani Brijen , Faust Russell A. , David Randy E. , Xagoraraki Irene
Published: February 2024

Background

Periodic bioinformatics-based screening of wastewater for assessing the diversity of potential human viral pathogens circulating in a given community may help to identify novel or potentially emerging infectious diseases. Any identified contigs related to novel or emerging viruses should be confirmed with targeted wastewater and clinical testing.

Results

During the COVID-19 pandemic, untreated wastewater samples were collected for a 1-year period from the Great Lakes Water Authority Wastewater Treatment Facility in Detroit, MI, USA, and viral population diversity from both centralized interceptor sites and localized neighborhood sewersheds was investigated. Clinical cases of the diseases caused by human viruses were tabulated and compared with data from viral wastewater monitoring. In addition to Betacoronavirus, comparison using assembled contigs against a custom Swiss-Prot human virus database indicated the potential prevalence of other pathogenic virus genera, including: Orthopoxvirus, Rhadinovirus, Parapoxvirus, Varicellovirus, Hepatovirus, Simplexvirus, Bocaparvovirus, Molluscipoxvirus, Parechovirus, Roseolovirus, Lymphocryptovirus, Alphavirus, Spumavirus, Lentivirus, Deltaretrovirus, Enterovirus, Kobuvirus, Gammaretrovirus, Cardiovirus, Erythroparvovirus, Salivirus, Rubivirus, Orthohepevirus, Cytomegalovirus, Norovirus, and Mamastrovirus. Four nearly complete genomes were recovered from the Astrovirus, Enterovirus, Norovirus and Betapolyomavirus genera and viral species were identified.

Conclusions

The presented findings in wastewater samples are primarily at the genus level and can serve as a preliminary “screening” tool that may serve as indication to initiate further testing for the confirmation of the presence of species that may be associated with human disease. Integrating innovative environmental microbiology technologies like metagenomic sequencing with viral epidemiology offers a significant opportunity to improve the monitoring of, and predictive intelligence for, pathogenic viruses, using wastewater.
Large-scale cultural heritage conservation and utilization based on cultural ecology corridors: a case study of the Dongjiang-Hanjiang River Basin in Guangdong, China
Authors: Sun Ying , Wang Yushun , Liu Lulu , Wei Zhiwei , Li Jialiang , Cheng Xi
Published: February 2024

In the field of world heritage conservation, there has been broad consensus on carrying out heritage conservation research on the basis of spatial integration and interregional and international cooperation. However, there are still many deficiencies in the integration of culture with the environment, regional economic and social development, and the regional, holistic and multimodal conservation and utilization of cultural heritage sites. In China, the Dongjiang-Hanjiang River Basin is a representative area of substantial cultural and ecological value for both Guangdong Province and the whole country. This paper uses the morphological spatial pattern analysis and the minimum cumulative resistance model to integrate cultural ecology sources and establish a cross-regional and large-scale cultural ecology network system that includes 1 main corridor, 22 important corridors and 17 secondary corridors. In addition, based on identified cultural landscape nodes and cultural ecology services, the economy of the cultural ecology corridor could be developed with large-scale co-construction, co-governance and shared working mechanisms to overcome administrative limits and realize the conservation and utilization of multimodal and large-scale heritage sites. This approach has strong theoretical and practical significance for innovative methods in cultural ecology research, as well as for new content in the research of Lingnan culture, ecosystem restoration, and the economic and social development of towns and villages. This article supplements unilateral studies of regional culture and ecology and demonstrates an in-depth application of cultural ecology theory.
Zero-knowledge proofs in education: a pathway to disability inclusion and equitable learning opportunities
Authors: Xu Xiao
Published: February 2024

In the evolving landscape of global education, the significance of inclusivity and equity has never been more important. Emphasizing the United Nation Sustainable Development Goal 4, this paper explores the innovative application of blockchain-powered Zero-Knowledge Proofs (ZKPs) technology in education, with a particular focus on disability inclusion. This study introduces a novel disability management system powered by Zero-Knowledge Succinct Non-Interactive Argument of Knowledge (zk-SNARK). This advanced system enables educational institutions to verify the status of students with disabilities without compromising their personal information, thereby preserving their privacy and reinforcing their identity. This paper evaluates the potential operational efficiency of this prototype system against the existing costs incurred by higher education institutions in disability schemes. It also examines the system’s potential to enhance self-disclosure among students with disability, which is pivotal for their academic success. By advocating for privacy and inclusivity, this study highlights the transformative potential of ZKP in creating an educational environment where students with disabilities can comfortably disclose their needs. This approach not only protects their confidentiality but also empowers them academically, aligning with the global commitment to accessible and inclusive education.