bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a clinical-dermoscopic model for the diagnosis of urticarial vasculitis
Authors: García-García B. , Aubán-Pariente J. , Munguía-Calzada P. , Vivanco B. , Argenziano G. , Vázquez-López F.
Published: April 2020

The clinical criteria for the diagnosis of urticarial vasculitis lack accuracy, according to previous studies. The aim of the study was to assess the accuracy of a clinical and a clinical-dermoscopic model for the differential diagnosis of chronic spontaneous urticaria (CSU) and urticarial vasculitis (UV). Dermoscopic images of lesions with histopathologically confirmed diagnosis of CSU and UV were evaluated for the presence of selected criteria (purpuric patches/globules (PG) and red linear vessels). Clinical criteria of CSU and UV were also registered. Univariate and adjusted odds ratios were calculated. Multivariate regression analyses were conducted separately for clinical variables (clinical diagnostic model) and for both clinical and dermoscopic variables (clinical-dermoscopic diagnostic model). 108 patients with CSU and 27 patients with UV were included in the study. The clinical-dermoscopic model notably showed higher diagnostic sensitivity than the clinical approach (63% vs. 44%). Dermoscopic purpuric patches/globules (PG) was the variable that better discriminated UV, increasing by 19-fold the odds for this diagnosis. In conclusion, dermoscopy helps the clinical discrimination between CSU and UV. The visualization of dermoscopic PG may contribute to optimize decisions regarding biopsy in patients with urticarial rashes.
Microfluidic Electroporation Coupling Pulses of Nanoseconds and Milliseconds to Facilitate Rapid Uptake and Enhanced Expression of DNA in Cell Therapy
Authors: Chang An-Yi , Liu Xuan , Tian Hong , Hua Liping , Yang Zhaogang , Wang Shengnian
Published: April 2020

Standard electroporation with pulses in milliseconds has been used as an effective tool to deliver drugs or genetic probes into cells, while irreversible electroporation with nanosecond pulses is explored to alter intracellular activities for pulse-induced apoptosis. A combination treatment, long nanosecond pulses followed by standard millisecond pulses, is adopted in this work to help facilitate DNA plasmids to cross both cell plasma membrane and nuclear membrane quickly to promote the transgene expression level and kinetics in both adherent and suspension cells. Nanosecond pulses with 400–800 ns duration are found effective on disrupting nuclear membrane to advance nuclear delivery of plasmid DNA. The additional microfluidic operation further helps suppress the negative impacts such as Joule heating and gas bubble evolution from common nanosecond pulse treatment that lead to high toxicity and/or ineffective transfection. Having appropriate order and little delay between the two types of treatment with different pulse duration is critical to guarantee the effectiveness: 2 folds or higher transfection efficiency enhancement and rapid transgene expression kinetics of GFP plasmids at no compromise of cell viability. The implementation of this new electroporation approach may benefit many biology studies and clinical practice that needs efficient delivery of exogenous probes.
Exploring yeast interactions through metabolic profiling
Authors: Roullier-Gall C. , David V. , Hemmler D. , Schmitt-Kopplin P. , Alexandre H.
Published: April 2020

As a complex microbial ecosystem, wine is a particularly interesting model for studying interactions between microorganisms as fermentation can be done by microbial consortia, a unique strain or mixed culture. The effect of a specific yeast strain on its environments is unique and characterized by its metabolites and their concentration. With its great resolution and excellent mass accuracy, ultrahigh resolution mass spectrometry (uHRMS) is the perfect tool to analyze the yeast metabolome at the end of alcoholic fermentation. This work reports the change in wine chemical composition from pure and mixed culture fermentation with Lachancea thermotolerans, Starmerella bacillaris, Metschnikowia pulcherrima and S. cerevisiae. We could clearly differentiate wines according to the yeast strain used in single cultures and markers, which reflect important differences between the yeast species, were extracted and annotated. Moreover, uHRMS revealed underlining intra species metabolomics differences, showing differences at the strain level between the two Starmerella bacillaris. Non volatile metabolomics analysis of single and sequential fermentations confirmed that mixed fermentations lead to a different composition. Distinct metabolites appeared in wines from sequential fermentation compared to single fermentation. This suggests that interactions between yeasts are not neutral.
Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin
Authors: Dong Wenbo , Oya Eriko , Zahedi Yasaman , Prasad Punit , Svensson J. Peter , Lennartsson Andreas , Ekwall Karl , Durand-Dubief Mickaël
Published: April 2020

Heterochromatin regulation is critical for genomic stability. Different H3K9 methylation states have been discovered, with distinct roles in heterochromatin formation and silencing. However, how the transition from H3K9me2 to H3K9me3 is controlled is still unclear. Here, we investigate the role of the conserved bromodomain AAA-ATPase, Abo1, involved in maintaining global nucleosome organisation in fission yeast. We identified several key factors involved in heterochromatin silencing that interact genetically with Abo1: histone deacetylase Clr3, H3K9 methyltransferase Clr4, and HP1 homolog Swi6. Cells lacking Abo1 cultivated at 30 °C exhibit an imbalance of H3K9me2 and H3K9me3 in heterochromatin. In abo1∆ cells, the centromeric constitutive heterochromatin has increased H3K9me2 but decreased H3K9me3 levels compared to wild-type. In contrast, facultative heterochromatin regions exhibit reduced H3K9me2 and H3K9me3 levels in abo1∆. Genome-wide analysis showed that abo1∆ cells have silencing defects in both the centromeres and subtelomeres, but not in a subset of heterochromatin islands in our condition. Thus, our work uncovers a role of Abo1 in stabilising directly or indirectly Clr4 recruitment to allow the H3K9me2 to H3K9me3 transition in heterochromatin.
Contrast-enhanced ultrasound features of malignant focal liver masses in dogs
Authors: Burti Silvia , Zotti Alessandro , Rubini Giuseppe , Orlandi Riccardo , Bargellini Paolo , Bonsembiante Federico , Banzato Tommaso
Published: April 2020

A total of 185 cases (150 retrospectively and 35 prospectively) of malignant liver masses were collected. In the retrospectively collected cases hyperenhancement during wash-in was the most common feature in HCCs but there was a high percentage of cases showing no enhancement or hypo/isoenhancement. ICCs displayed a large variety of contrast enhancement patterns and, although statically significant differences between ICCs and HCCs were evident, no clear distinction between these two pathologies was possible based only on their CEUS appearance. Sarcomas displayed all the possible degrees of wash-in enhancement with non-enhancing being the most common appearance. Metastases displayed all the possible contrast-enhancement patterns, with the most common being hyperenhancement in the wash-in phase followed by hypoenhancement in the wash-out phase. A decision tree was developed based on the features of the retrospectively selected cases. Based on the developed decision tree 27/35 prospectively collected cases were correctly classified. Even if some significant differences among groups were evident, all the histotypes displayed all the possible patterns of contrast enhancement, and, therefore, the differentiation of liver masses in dogs based only on their CEUS features is not feasible and, therefore, cytology or histopathology is required.
Artificial intelligence for the diagnosis of heart failure
Authors: Choi Dong-Ju , Park Jin Joo , Ali Taqdir , Lee Sungyoung
Published: April 2020

The diagnosis of heart failure can be difficult, even for heart failure specialists. Artificial Intelligence-Clinical Decision Support System (AI-CDSS) has the potential to assist physicians in heart failure diagnosis. The aim of this work was to evaluate the diagnostic accuracy of an AI-CDSS for heart failure. AI-CDSS for cardiology was developed with a hybrid (expert-driven and machine-learning-driven) approach of knowledge acquisition to evolve the knowledge base with heart failure diagnosis. A retrospective cohort of 1198 patients with and without heart failure was used for the development of AI-CDSS (training dataset, n = 600) and to test the performance (test dataset, n = 598). A prospective clinical pilot study of 97 patients with dyspnea was used to assess the diagnostic accuracy of AI-CDSS compared with that of non-heart failure specialists. The concordance rate between AI-CDSS and heart failure specialists was evaluated. In retrospective cohort, the concordance rate was 98.3% in the test dataset. The concordance rate for patients with heart failure with reduced ejection fraction, heart failure with mid-range ejection fraction, heart failure with preserved ejection fraction, and no heart failure was 100%, 100%, 99.6%, and 91.7%, respectively. In a prospective pilot study of 97 patients presenting with dyspnea to the outpatient clinic, 44% had heart failure. The concordance rate between AI-CDSS and heart failure specialists was 98%, whereas that between non-heart failure specialists and heart failure specialists was 76%. In conclusion, AI-CDSS showed a high diagnostic accuracy for heart failure. Therefore, AI-CDSS may be useful for the diagnosis of heart failure, especially when heart failure specialists are not available.
From metal–organic frameworks to single/dual-atom and cluster metal catalysts for energy applications
Authors: Hou Chun-Chao , Wang Hao-Fan , Li Caixia , Xu Qiang
Published: April 2020

Single/dual-atom and cluster metal catalysts have emerged as a new frontier in catalysis. This review highlights recent advances and provides a state-of-the-art understanding of MOF-based synthesis strategies and their prospective applications.
Amorphous calcium organophosphate nanoshells as potential carriers for drug delivery to Ca2+-enriched surfaces
Authors: Perez Enriquez Darlin J. , Dell’ Arciprete María L. , Dittler María L. , Miñan Alejandro , Prieto Eduardo , Gonzalez Mónica C.
Published: April 2020

Our amorphous calcium organophosphate nanoshells are prone to agglomerate and disassemble when Ca2+ions are present in the solution and on surfaces. This have great implications for targeting and drug release in Ca-rich environments, such as bone.
Coupling of digital image processing and three-way calibration to assist a paper-based sensor for determination of nitrite in food samples
Authors: Almasvandi Zohreh , Vahidinia Ali , Heshmati Ali , Zangeneh Mohammad Mahdi , Goicoechea Hector C. , Jalalvand Ali R.
Published: April 2020

In this work, a novel and very interesting analytical methodology based on coupling of digital image processing and three-way calibration has been developed for determination of nitrite in food samples.
Techno-economic analysis and comprehensive optimization of anon-sitehydrogen refuelling station system using ammonia: hybrid hydrogen purification with both high H2purity and high recovery
Authors: Lin Li , Tian Yao , Su Wenbin , Luo Yu , Chen Chongqi , Jiang Lilong
Published: April 2020

A carbon-freeon-sitehydrogen refuelling station using ammonia and hybrid “PSA-to-membrane” separation can produce more than 99.97%-purity and more than 95%-recovery hydrogen with an 85.37% efficiency and a cost of 4.31 € per kg.
A one-dimensional dual emissive hybrid perovskite with flexibly tunable white-light emission
Authors: Li Dong , Wu Wentao , Wang Sasa , Zhang Xinyuan , Li Lina , Yao Yunpeng , Peng Yu , Luo Junhua
Published: April 2020

Tunable white-light emission, from “cold” white light to “warm” white light, is facilely achieved using the one-dimensional perovskite (N-AEP)2Pb2Cl10·H2O.
Selection and optimization of protein and carbohydrate assays for the characterization of marine biofouling
Authors: Richards Chloe , O'Connor Nicole , Jose Diveena , Barrett Alan , Regan Fiona
Published: April 2020

Biofilms comprise a set of microorganisms attached to a surface through extracellular polymeric substances (EPS). Development of reliable analytical assays are valuable in determining the rate of biofilm attachment on surfaces.
Rhodium-catalyzed Sommelet–Hauser type rearrangement of α-diazoimines: synthesis of functionalized enamides
Authors: Reddy Angula Chandra Shekar , Ramachandran Kuppan , Reddy Palagulla Maheswar , Anbarasan Pazhamalai
Published: April 2020

An efficient rhodium catalyzed Sommelet–Hauser type rearrangement of sulfur ylides derived from α-thioesters and N-sulfonyl-1,2,3-triazoles has been successfully accomplished for the synthesis of various functionalized enamides.
Access to 4-substituted isothiazoles through three-component cascade annulation and their application in C–H activation
Authors: Huang Guoling , Li Jian , Ji Xiaoliang , Chen Lu , Liu Qiang , Chen Xiuwen , Huang Yubing , Li Yibiao
Published: April 2020

The use of EtOCS2k enabled the annulation of isopropene derivatives with NH4I, affording various 4-substituted isothiazoles in good yields.
Carbohydrate supramolecular chemistry: beyond the multivalent effect
Authors: González-Cuesta Manuel , Ortiz Mellet Carmen , García Fernández José M.
Published: April 2020

(Hetero)multivalency acts as a multichannel switch that shapes the supramolecular properties of carbohydrates in an intrinsically multifactorial biological context.
Study of the surface species during thermal and plasma-enhanced atomic layer deposition of titanium oxide films using in situ IR-spectroscopy and in vacuo X-ray photoelectron spectroscopy
Authors: Vandenbroucke Sofie S. T. , Levrau Elisabeth , Minjauw Matthias M. , Van Daele Michiel , Solano Eduardo , Vos Rita , Dendooven Jolien , Detavernier Christophe
Published: April 2020

By the powerful combination of in situ FTIR and in vacuo XPS, the surface species during ALD of TDMAT with different reactants could be identified.
Catalytic dioxygen reduction mediated by a tetranuclear cobalt complex supported on a stannoxane core
Authors: Chandra Anirban , Mebs Stefan , Kundu Subrata , Kuhlmann Uwe , Hildebrandt Peter , Dau Holger , Ray Kallol
Published: April 2020

The synthesis, spectroscopic characterization and DFT calculations of a tetranuclear cobalt complex supported on a stannoxane core, and its catalytic O2-reduction ability are reported.
Hydrogen bonding interactions in single component molecular conductors based on metal (Ni, Au) bis(dithiolene) complexes
Authors: Hachem Hadi , Bellec Nathalie , Fourmigué Marc , Lorcy Dominique
Published: April 2020

Nickel (closed-shell) or gold (radical) bis(dithiolene) neutral complexes, functionalized with hydroxyethyl and thiazole moieties, afford hydrogen-bonded single component conductors.
A new microfluidic model that allows monitoring of complex vascular structures and cell interactions in a 3D biological matrix
Authors: van Dijk Christian G. M. , Brandt Maarten M. , Poulis Nikolaos , Anten Jonas , van der Moolen Matthijs , Kramer Liana , Homburg Erik F. G. A. , Louzao-Martinez Laura , Pei Jiayi , Krebber Merle M. , van Balkom Bas W. M. , de Graaf Petra , Duncker Dirk J. , Verhaar Marianne C. , Luttge Regina , Cheng Caroline
Published: April 2020

We present a microfluidic vascular device. Vascular cells in a 3D-ECM environment support hemodynamic flow and enable monocyte interaction.
A bespoke microfluidic pharmacokinetic compartment model for drug absorption using artificial cell membranes
Authors: Korner Jaime L. , Stephenson Elanna B. , Elvira Katherine S.
Published: April 2020

A new type of pharmacokinetic compartment model using artificial cell membranes that predicts intestinal absorption three times more accurately than the current state of the art.
Each phenyl group performs its own functions on luminescence: phenyl substituted effect in tetraphenylpyrazine
Authors: Wu Haozhong , Song Xiaojuan , Zhang Bing , Wang Zhiming , Zhang Tian , Qin Anjun , Tang Ben Zhong
Published: April 2020

Three phenyl groups of TPP derivatives affect the luminescence process in various modes. TPPs show AIE thanks to the ortho substituted groups, while meta and para groups can modulate their luminescence in different extents.
A hydrazide organogelator for fluoride sensing with hyperchromicity and gel-to-sol transition
Authors: Park Sangwoo , Ju Jeewon , Lee Young Ju , Lee Sang-Yup
Published: April 2020

A low-molecular-weight organogelator containing hydrazide groups, DPH, exhibited considerable selectivity and sensitivity for fluoride. The optical sensing of fluoride was demonstrated with the systematic study on the sensing mechanism.
High stability and visible-light photocatalysis in novel two-dimensional monolayer silicon and germanium mononitride semiconductors: first-principles study
Authors: Zhang Kaining , Li Nan
Published: April 2020

The two-dimensional SiN and GeN semiconductors are expected to become novel photocatalysts for water-splitting.
Preparation of a nanoscale homogeneous energetic lead azides@porous carbon hybrid with high ignition ability by in situ synthesis
Authors: Yan Zhenzhan , Yang Li , Han Ji-Min , Song Naimeng , Liu Jianchao
Published: April 2020

The ever-increasing demand for miniaturized explosive systems urgently calls for better performance studies through the synthesis of novel nanoscale materials.
Effects of metal cation doping in CeO2 support on catalytic methane steam reforming at low temperature in an electric field
Authors: Takahashi Ayako , Inagaki Reona , Torimoto Maki , Hisai Yudai , Matsuda Taku , Ma Quanbao , Seo Jeong Gil , Higo Takuma , Tsuneki Hideaki , Ogo Shuhei , Norby Truls , Sekine Yasushi
Published: April 2020

Temperature dependence of electron/ion conductivity of Pd/CeO2 and Pd/Al-CeO2 under wet conditions with application of an electric field.
The intellectual demands of the intended chemistry curriculum in Czechia, Finland, and Turkey: a comparative analysis based on the revised Bloom's taxonomy
Authors: Elmas Rıdvan , Rusek Martin , Lindell Anssi , Nieminen Pasi , Kasapoğlu Koray , Bílek Martin
Published: April 2020

Understanding the intellectual demands of an intended curriculum is crucial as it defines the frames for teaching and learning processes and practice during lessons. In this study, upper-secondary school chemistry curricula contents in Czechia, Finland, and Turkey were analysed, and their objectives were compared using the Revised Bloom's Taxonomy (RBT). The intellectual demands were examined analysing the action verbs in the three curricula objectives based on their association with the intended cognitive process dimensions in the RBT. The Turkish upper-secondary chemistry curriculum was found to be more structured, detailed, and containing more objectives than the Czech and Finnish curricula. The domineering objectives in cognitive demands were understand (77.2%) and analyse and apply (both 7.1%). Conceptual items dominated (59.8%) with procedural items identified (29.1%). Also, there are five metacognitive items (3.9%). The Czech curriculum, compared to the Finnish and Turkish curricula, does not take modern trends in the field of chemistry into account. The cognitive demands in the Czech curriculum were skewed toward apply (40%) with understand and evaluate accordingly represented by 20%. Conceptual items dominate with a 53.3% of occurrence. In the Finnish curriculum, the cognitive demands were skewed toward apply (47.1%) with create (23.5%) and understand (17.6%). Procedural (35.3%) domains predominate, although metacognitive objectives represent a significant share (23.5%) too. These findings from the contents and intellectual demands of the curricula in each of the three countries have the potential to help teachers and other actors in education design the interventions and assessments implemented in the classes. Comparing the distribution of intellectual demands between the countries provides an international reference for educational reforms in hand in many countries.
Directed motion of an impinging water droplet—seesaw effect
Authors: Wang Shun , Li Hailong , Duan Hu , Cui Yingtao , Sun Heng , Zhang Mengjiao , Zheng Xianfu , Song Meirong , Li He , Dong Zhichao , Ding Hang , Jiang Lei
Published: April 2020

This study discovered a “seesaw effect” in the underlying physics of the directional bouncing behavior of a droplet on superhydrophobic heterogeneous surfaces, leading to projectile motion.
Phosphate ion and oxygen defect-modulated nickel cobaltite nanowires: a bifunctional cathode for flexible hybrid supercapacitors and microbial fuel cells
Authors: Qiu Wenda , Zhou Quanhua , Xiao Hongbing , Zhou Chun , He Wenting , Li Yu , Lu Xihong
Published: April 2020

The exploration of efficient and cost-effective cathodes for flexible hybrid supercapacitors (HSCs) and microbial fuel cells (MFCs) is highly desirable but challenging.
Tuning of endosomal escape and gene expression by functional groups, molecular weight and transfection medium: a structure–activity relationship study
Authors: Richter Friederike , Martin Liam , Leer Katharina , Moek Elisabeth , Hausig Franziska , Brendel Johannes C. , Traeger Anja
Published: April 2020

A library of cationic polyacrylamide homopolymers was synthesized and their gene delivery, endosomal release, and interaction with endosome-specific lipids were investigated. The guanidinium-containing polymers were most efficient.
Chemoreactive nanomedicine
Authors: Feng Wei , Chen Yu
Published: April 2020

Triggering specific chemical reactions in the disease microenvironment can produce species for disease treatment that have high theranostic performance and low side effects on healthy cells/tissues.
A biochemical comparison of the lung, colonic, brain, renal, and ovarian cancer cell lines using 1H-NMR spectroscopy
Authors: Hu Cong , Liu Zhigang , Zhao Hailin , Wu Lingzhi , Lian Qingquan , Ma Daqing , Li Jia V.
Published: April 2020

Cancer cell lines are often used for cancer research. However, continuous genetic instability-induced heterogeneity of cell lines can hinder the reproducibility of cancer research. Molecular profiling approaches including transcriptomics, chromatin modification profiling, and proteomics are used to evaluate the phenotypic characteristics of cell lines. However, these do not reflect the metabolic function at the molecular level. Metabolic phenotyping is a powerful tool to profile the biochemical composition of cell lines. In the present study, 1H-NMR spectroscopy-based metabolic phenotyping was used to detect metabolic differences among five cancer cell lines, namely, lung (A549), colonic (Caco2), brain (H4), renal (RCC), and ovarian (SKOV3) cancer cells. The concentrations of choline, creatine, lactate, alanine, fumarate and succinate varied remarkably among different cell types. The significantly higher intracellular concentrations of glutathione, myo-inositol, and phosphocholine were found in the SKOV3 cell line relative to other cell lines. The concentration of glutamate was higher in both SKOV3 and RCC cells compared with other cell lines. For cell culture media analysis, isopropanol was found to be the highest in RCC media, followed by A549 and SKOV3 media, while acetone was the highest in A549, followed by RCC and SKOV3. These results demonstrated that 1H-NMR-based metabolic phenotyping approach allows us to characterize specific metabolic signatures of cancer cell lines and provides phenotypical information of cellular metabolism.
m6A-dependent up-regulation of DRG1 by METTL3 and ELAVL1 promotes growth, migration, and colony formation in osteosarcoma
Authors: Ling Zhian , Chen Liangjun , Zhao Jinmin
Published: April 2020

Osteosarcoma (OS) is a malignant tumor commonly observed in children and adolescents. Developmentally regulated GTP-binding protein (DRG) 1 plays an important role in embryonic development; aberrantly expressed DRG1 has been associated with pathological processes in cancer. The present study aimed to explore the role of DRG1 in OS and the mechanisms underlying DRG1 overexpression in OS. Clinical studies were performed to evaluate Drg1 expression in OS tissues and to identify a correlation between Drg1 expression and the clinicopathological features in patients with OS. Drg1 was knocked down in OS cells to determine its effects on cell viability, cell cycle distribution, apoptosis, migration, invasion, and colony formation rate. METTL3 and ELAVL1 were also silenced to determine their effects on the levels of N6-methyladenosine (m6A), RNA stability, and Drg1 expression. Higher levels of Drg1 mRNA and protein were observed in OS tissues than those in paracancerous tissues. High expression of DRG1 was associated with large tumor size and advanced clinical stages in OS. Silencing of Drg1 resulted in decreased viability and inhibition of the migration and colony formation abilities of OS cells; it also resulted in cell cycle arrest in the G2/M stage and induced apoptosis. Knockdown of METTL3 led to decreased m6A and Drg1 mRNA levels. ELAVL1 knockdown impaired the stability of DRG1 mRNA, thereby reducing both the mRNA and protein levels of DRG1. In all, DRG1 exerted tumorigenic effects in OS, and the up-regulation of DRG1 in OS was induced by METTL3 and ELAVL1 in an m6A-dependent manner.
Methods for protein delivery into cells: from current approaches to future perspectives
Authors: Chau Chalmers , Actis Paolo , Hewitt Eric
Published: April 2020

The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.
Dual‐output unity power factor rectifier power block
Authors: Rana Mandeep Singh , Mishra Santanu K.
Published: April 2020

Unity power factor rectifiers find applications in modern utility systems and consumer electronic devices. However, a dedicated rectifier is required for a given load. In this study, a Unity Power Factor (UFP) power block is described which can generate two DC outputs, one buck and one boost, with a reduced number of switches and associated driver circuits. The topology adapts well with conventional control as it retains the dynamic behaviour of a buck and boost topology. While supplying simultaneous loads it exhibits minimal cross‐regulation interference between the outputs. The controller implementation for the proposed power block is described and its difference with the conventional Power Factor Correction (PFC) controller is outlined. The topology along with its control to regulate the outputs and force UPF operation are validated using a 200 W prototype with 110 V utility input and 180–24 V DC outputs.
X-ray andγ-ray orbital variability from theγ-ray binary HESS J1832−093
Authors: Martí-Devesa G. , Reimer O.
Published: April 2020

Context.γ-ray binaries are systems composed of a massive star and a compact object whose interaction leads to particle acceleration up to relativistic energies. In the last fifteen years, a few binaries have been found to emit at high energies, but their number is still low. The TeV source HESS J1832−093 has been proposed as a binary candidate, although its nature is unclear. Neither a GeV counterpart nor a period was detected.

Aims.The purpose of this work is to search for a GeV counterpart to understand the origin of the TeV signal detected by H.E.S.S. For an unambiguous identification of its binary nature, finding an orbital modulation is crucial.

Methods.We analysed data spanning more than 10 years from theFermiLarge Area Telescope (Fermi-LAT), together withSwiftarchival observations taken between 2015 and 2018, using both the X-Ray Telescope and UV/Optical Telescope. We searched for periodicities in both X-ray and GeV bands.

Results.We find a periodic modulation of ∼ 86 days in the X-ray source candidate counterpart XMMU J183245−0921539, together with indications ofγ-ray modulation with a compatible period in the GeV candidate counterpart 4FGL J1832.9−0913. Neither an optical nor a UV counterpart is found at the X-ray source location. The overall spectral energy distribution strongly resembles the knownγ-ray binary HESS J0632+057.

Conclusions.Both the spectral energy distribution and the discovery of an orbital period allow the identification of the TeV source HESS J1832−093 as a new member of theγ-ray binary class.
SESAME project: advancements in liquid metal thermal hydraulics experiments and simulations
Authors: Tarantino Mariano , Roelofs Ferry , Shams Afaque , Batta Abdalla , Moreau Vincent , Di Piazza Ivan , Gershenfeld Antoine , Planquart Philippe
Published: April 2020

Liquid metal cooled reactors are envisaged to play an important role in the future of nuclear energy production because of their possibility to use natural resources efficiently and to reduce the volume and lifetime of nuclear waste. Sodium and Liquid lead (-alloys) are considered the short and long term solution respectively, as coolant in GEN-IV reactor. Thermal-hydraulics of liquid metals plays a key role in the design and safety assessments of these reactors. Therefore, this is the main topic of a large European collaborative program (the Horizon 2020 SESAME) sponsored by the European Commission. This paper will present the progress in the project with respect to liquid metal cooled reactor thermal-hydraulics (liquid metal heat transfer, fuel assembly thermal-hydraulics, pool thermal-hydraulics, and system thermal-hydraulics). New reference data, both experimental and high-fidelity numerical data is being generated. And finally, when considering the system scale, the purpose is to validate and improve system thermal-hydraulics models and codes, but also to further develop and validate multi-scale approaches under development.
Systemische Therapieoptionen bei lokal fortgeschrittenen und metastasierten myxoiden, dedifferenzierten und pleomorphen Liposarkomen
Authors: Pink Daniel , West Antje , Andreou Dimosthenis
Published: April 2020

Noch vor wenigen Jahren haben alle Patienten mit metastasierten Weichteilsarkomen die gleiche Chemotherapie erhalten. Infolge der Erkenntnis, dass es sich bei den verschiedenen Sarkomsubtypen um unterschiedliche Erkrankungen mit unterschiedlichen genetischen Veränderungen und einem unterschiedlichen biologischen Verhalten handelt, wurden allerdings in letzter Zeit zunehmend subtypspezifische medikamentöse Therapieprotokolle eingesetzt. Selbiges gilt auch für die verschiedenen Subtypen von Liposarkomen – das myxoide/rundzellige Liposarkom, das dedifferenzierte Liposarkom und das pleomorphe Liposarkom. In diesem Beitrag werden publizierte Daten und Erfahrungen mit unterschiedlichen systemischen Therapieoptionen in den verschiedenen Therapielinien sowie ein Überblick über experimentelle Therapieansätze präsentiert.
Design and Usability of an Electronic Health Record—Integrated, Point-of-Care, Clinical Decision Support Tool for Modeling and Simulation of Antihemophilic Factors
Authors: Abdel-Rahman Susan M. , Gill Harpreet , Carpenter Shannon L. , Gueye Pathe , Wicklund Brian , Breitkreutz Matt , Ghosh Arindam , Kollu Avinash
Published: April 2020

Background With the consequences of inadequate dosing ranging from increased bleeding risk to excessive drug costs and undesirable administration regimens, the antihemophilic factors are uniquely suited to dose individualization. However, existing options for individualization are limited and exist outside the flow of care. We developed clinical decision support (CDS) software that is integrated with our electronic health record (EHR) and designed to streamline the process for our hematology providers.

Objectives The aim of this study is to develop and examine the usability of a CDS tool for antihemophilic factor dose individualization.

Methods Our development strategy was based on the features associated with successful CDS tools and driven by a formal requirements analysis. The back-end code was based on algorithms developed for manual individualization and unit tested with 23,000 simulated patient profiles created from the range of patient-derived pharmacokinetic parameter estimates defined in children and adults. A 296-item heuristic checklist was used to guide design of the front-end user interface. Content experts and end-users were recruited to participate in traditional usability testing under an institutional review board approved protocol.

Results CDS software was developed to systematically walk the point-of-care clinician through dose individualization after seamlessly importing the requisite patient data from the EHR. Classical and population pharmacokinetic approaches were incorporated with clearly displayed estimates of reliability and uncertainty. Users can perform simulations for prophylaxis and acute bleeds by providing two of four therapeutic targets. Testers were highly satisfied with our CDS and quickly became proficient with the tool.

Conclusion With early and broad stakeholder engagement, we developed a CDS tool for hematology provider that affords seamless transition from patient assessment, to pharmacokinetic modeling and simulation, and subsequent dose selection.
A Pilot Study of a Novel Fixation Technique for Fixation of Comminuted Patellar Fractures: Arthroscopic-Controlled Reduction and Circular External Fixation
Authors: Neyisci Cagri , Erdem Yusuf , Kilic Erden , Arsenishvili Arsen , Kürklü Mustafa
Published: April 2020

Patella fractures represent for 0.5 to 1.5% of all bony injuries in adults. Open reduction and the modified tension-band technique is the most common surgical technique used for patellar fractures. The purpose of this study is to present the outcomes of 26 comminuted patellar fractures treated with circular external fixator (CEF) under arthroscopic control and discuss its potential advantages over conventional surgical methods. This retrospective study included 26 patients who had closed comminuted patellar fractures and treated by CEF under arthroscopic control between January 2002 and March 2016. All patients treated with this technique were involved to the study as a consecutive series. Patients with noncomminuted transverse fractures were excluded, because they were treated with a different technique. Of the 26 patients 22 were male, 4 were female with the mean age of 33.5 years (range, 16–56 years). Patients were followed for 20 to 28 months (mean, 22 months). The mean time to union and the duration of fixation with the CEF ring was 12 weeks (range, 6–15 weeks). The mean Lysholm's score was 45 (range, 35–58) at the 10th postoperative day, which increased to 51 (range, 40–68) at the end of the first postoperative month and increased to 95 (range, 90–100) 1 month after CEF ring removal. Minor pin tract infection by pin-skin irritation was observed in nine patients. In one patient, refracture occurred due to a fall 19 days after CEF removal. CEF appears to be a safe and effective treatment for comminuted patellar fractures with a high union rate and minimal complications. It is safe and effective, as it allows short hospital stay and avoids a second surgery for removal of the instrument. Early rehabilitation with full weight-bearing promotes rapid recovery and quick return to work. Patients do not have a large unaesthetic scar on the anterior of the knee.
Closed Incisional Negative Pressure Wound Therapy at Flap Suture Line: An Innovative Approach for Improving Outcomes in Suboptimal Wound Conditions
Authors: Naalla Ravikiran , Bhushan Smriti , Abedin Minhaj Ul , Bichpuriya Ashish Dhanraj , Singhal Maneesh
Published: April 2020

Background Persistent dead space following flap cover is a frequently encountered challenge following the reconstruction of complex wounds. It may lead to a hematoma, seroma, wound infection, and wound dehiscence. Wound dehiscence could be a devastating complication. Closed incisional negative pressure wound therapy (ciNPWT) over the surgical incisions was found to reduce surgical site infection (SSI) and wound dehiscence. We applied this principle at the closed flap suture line and through this article, we share the indications, technique, and outcomes.

Methods A retrospective analysis (January 2018–June 2019), in which selected high-risk patients who underwent ciNPWT at the flap suture following complex reconstruction (pedicled or free flap) were included in the study. The indications include deep incisional/organ SSI after debridement and flap coverage, persistent dead space following flap coverage, chronic osteomyelitis. Patients were analyzed in the follow-up period in terms of complications, wound healing.

Results Nine patients underwent ciNPWT over the flap suture line. The mean age was 32.2 years (range: 10–48 years). The mean duration of the NPWT application was 7.3 days (range: 3–21 days). Three of the nine patients had flap-related minor complications. One patient had marginal flap necrosis and required skin grafting, one patient had minor wound dehiscence (1 cm) which required secondary skin suturing and one patient had chronic discharging sinus related to osteomyelitis of ischium, which subsequently healed with antibiotics and local wound care. None of the patients had NPWT-related complications.

Conclusion Closed incisional NPWT decreases the untoward effects of dead space following the reconstruction of complex wounds. The incidence of SSI and wound gaping can be reduced.
The Perside Language of Shiraz Jewry: A Historical-Comparative Phonology
Authors: Borjian Habib
Published: April 2020

This study concerns the native language of Shirazi Jews, most of whom live in diasporic communities outside Iran. The language Judeo-Shirazi belongs to the Southwest Iranian group, as do most other native languages spoken in southern Iran. As such, Judeo-Shirazi shows general agreements with native rural varieties spoken in inland Fārs. There are, however, phonological features suggesting that Judeo-Shirazi is an insular survivor of the Medieval Shirazi language, from which a sizable literature has survived dating back to the fifteenth century.
Seasonal prediction of Euro-Atlantic teleconnections from multiple systems
Authors: Lledó Llorenç , Cionni Irene , Torralba Verónica , Bretonnière Pierre-Antoine , Samsó Margarida
Published: April 2020

Seasonal mean atmospheric circulation in Europe can vary substantially from year to year. This diversity of conditions impacts many socioeconomic sectors. Teleconnection indices can be used to characterize this seasonal variability, while seasonal forecasts of those indices offer the opportunity to take adaptation actions a few months in advance. For instance, the North Atlantic Oscillation has proven useful as a proxy for atmospheric effects in several sectors, and dynamical forecasts of its evolution in winter have been shown skillful. However the NAO only characterizes part of this seasonal circulation anomalies, and other teleconnections such as the East Atlantic, the East Atlantic Western Russia or the Scandinavian Pattern also play an important role in shaping atmospheric conditions in the continent throughout the year. This paper explores the quality of seasonal forecasts of these four teleconnection indices for the four seasons of the year, derived from five different seasonal prediction systems. We find that several teleconnection indices can be skillfully predicted in advance in winter, spring and summer. We also show that there is no single prediction system that performs better than the others for all seasons and teleconnections, and that a multi-system approach produces results that are as good as the best of the systems.
Pharmacology potency of thin layer chromatography steroid isolates of Chlorella sp. chloroform fraction
Authors: Megawati D S , Fasya A G , Pratiwi R A , Maghfiroh N
Published: April 2020

Al-Qur’an surah as Syu’ara verse 7 shows that Allah SWT grew many good and advantages plants, such as microalgae Chlorella sp. Chlorella sp. are contained some secondary metabolites, such as steroid compounds. Purpose of this research is to know toxicity levels and antioxidant activity steroid compound of chloroform and ethyl acetate fractions from hydrolyzed methanol extract of microalgae Chlorella sp. Cultivation is conducted on a laboratory scale using Sprouts Extract Medium (SEM) 4% and harvesting conducted every 10th day. Dry Chlorella sp. biomass was macerated using methanol solvent. The extract then hydrolyzed with 2 N of HCl and partitioned with chloroform, then the separation using Thin Layer Chromatography (TLC). Isolates conducted toxicity tests using Brine Shrimp Lethality Test (BSLT) and antioxidant activity test with the DPPH method. The toxicity test showed that TLC steroid isolates of chloroform fraction had LC50 values 8.796 (C1), 8.53 (C4) and 4.53 ppm (C5). The antioxidants test showed that TLC steroid isolates of chloroform fraction had had a value of EC50 is 2.26 1019 (C1) 163.10 ppm (C4) and 38.96 ppm (C5). Identification of steroid compounds with UV-VIS spectrophotometer has a maximum wavelength of 250.50, 270.5 and 281 nm. Identification with FTIR showed functional groups C=O (carbonyl), C-O (ether), -C(CH3)2 (geminal dimethyl), C=C and = CH (alkenes) attributed to steroid compounds.
The effect of synthesis time to particle size of Bi4Ti3O12 which synthesized using molten single salt NaCl method
Authors: Januari T , Aini N , Barroroh H , Prasetyo A
Published: April 2020

Bi4Ti3O12 was synthesized by the molten salt method using single salt NaCl with calcination time variations: 2, 4, 8, and 16 hours at 900 °C. The effect of time synthesis on particle size and morphology was investigated. The X-ray diffraction data showed that the sample Bi4Ti3O12 was successfully synthesis and there is not found any impurities. The image from scanning electron microscopy showed that the shape of Bi4Ti3O12 particle is plate-like and particle size increases as calcination time.
Digital workplace: digital transformation for environmental sustainability
Authors: Yalina N , Rozas I S
Published: April 2020

Environmental Sustainability becomes a major issue around the world. Energy consumption and air pollution are some important issues that have been discussed among scholars and practitioners. The purpose of this paper is to examine how digital workplace implementation could contribute to empower environmental sustainability, why this is important and how this can be achieved. We collected the data from UIN Sunan Ampel Surabaya and do the simulation if their employees implement the digital workplace for three cases, 50% of the farthest employees, 50% of the nearest employees, and 50% on random. The efficiency of fuel used obtain was 44.39%, 5,61%, and 22.2% respectively. From the simulation, it can be concluded that the application of the digital workplace can be an alternative in the framework of global environmental sustainability.
New compounds of pregnanone from Calotropis gigantea roots actively against colon cancer cell WiDr through cell cycle inhibition
Authors: Mutiah R , Widyawaruyanti A , Sukardiman
Published: April 2020

Calotropis gigantea (L.) W. Aiton (C. gigantea) is a medicinal plant that has been empirically proven to have anticancer activity. In a previous study, it showed that the fraction of ethyl acetate from the root part of C. gigantea had higher anticancer activity than the other fractions. It suspected that the ethyl acetate fraction of C. gigantea root contained active compounds that has anticancer properties. This study aimed to determine the anticancer activity of active compounds from the ethyl acetate fraction of C. gigantea root regarding induction of apoptosis, cell cycle arrest, and expression of caspase-8 colon cancer cell WiDr. Isolation of the active compounds from the ethyl acetate fraction of C. gigantea root was carried out using Bioassay-guided Isolation method. Identification of active compounds was using NMR-1H, NMR-13C, HMBC, HMQC and UPLCMS/MS methods. The anticancer activity test of the identified compounds performed by using MTT method. The induction of apoptotic and cell cycle arrest evaluated by a flow cytometry method. The result of this study showed two active compounds were identified namely (1) (Pregnanon-5-en, 3,14,17 trihydroxy-12- (4’-cyclohexyl benzoate) -, (3β, 12β, 14β) - (9CI), (2) Pregn-5-en-20-one, 3,8,14-trihydroxy-12 - [(4’-hydroxy benzoyl) oxy] -, (3β, 12β, 14β, 17α) - (9CI). Both compounds inhibited the growth of colon cancer cell WiDr with IC50 values respectively were 15.89 μg/mL and 0.77 μg/mL. Both compounds increased the induction of apoptotic by increasing sub-G1, S, and G2-M following depletion of G0-G1 phase accumulation.
Disinfecting technology of Camellia sinensis L inoculants through in vitro culture
Authors: Sutini  , Widiwurjani  , Augustien N , Pribadi DU , Djoko A P
Published: April 2020

Leaves of the Camellia sinensis plant is a very economically valuable commodity and can be applied to various fields. Such as in the fields of agriculture, food and beverage and medicine. The problem that is found in the land area is the presence of plants that are more than tens of years old that need to be plant rejuvenated. Rejuvenation with grafting and cutting techniques is strongly influenced by the climate and extensive agricultural land. To overcome this problem, in vitro culture techniques are applied as an effort to conserving/producing plant seeds that are free of disease pests, controlled environments and with narrow land or laboratory scale. This research aimed to determine the inoculant of Camellia sinensis which gave the best results on the disinfecting through in vitro culture technique. The method used in the research was using two types of inoculants, they are shoot buds and young shoots in the disinfecting process, and each process was repeated fifteen times. The results obtained were that the disinfecting of Camellia sinensis plants with shoot bud inoculants showed the lowest level of contamination with the number of growing inoculants close to 85 percent in twelve weeks of inoculant harvesting.
Release test of N, P, and K of complete slow release fertilizer (PUKAP JESTRO-1) and its effect on the growth of young siam citrus (Citrus nobilis Lour.)
Authors: Sutopo  , Aji T G , Budiyati E
Published: April 2020

The research aimed to study the release of N, P, K elements from slow release complete fertilizer (PUKAP JESTRO-1) and its effect on the growth of Siam citrus in a high density planting system (HDP). The release test of PUKAP JESTRO-1 was carried out in a laboratory, where the remaining N, P, K content in fertilizer was measured periodically. The influence test of PUKAP JESTRO-1, which compared with conventional fertilizer in the form of a single fertilizer and NPK compound fertilizer, was carried out on a 1-month old Siam citrus planted on Grumusol (vertisol). The study used randomized block design with 5 treatments and 3 replications, namely 400 g/plant/year mixed fertilizer (200 g Urea + 150 g SP36 + 50 g KCl); 200 g/plant/year PUKAP JESTRO-1; 300 g/plant/year PUKAP JESTRO-1; 400 g/plant/year PUKAP JESTRO-1; 400 g/plant/year NPK YaraMila (16-16-16). The experimental results showed that PUKAP JESTRO-1 released N, P, and K until the fourth, tenth, and eighth week respectively. Application of PUKAP JESTRO-1 at 25% lower dosages produced better plant height compared to mixed fertilizer applications but did not significantly increase leaf N, P, and K levels due to the effect of dilution of elements in plant tissue.