bluuebunny/crossref_metadata_2025 huggingface dataset:
Association Between Chemotherapy and Survival in T1 Colon Cancer With Lymph Node Metastasis: A Propensity-Score Matched Analysis
Authors: Yan Wangxin , Zhou Huizhen , Shi Si , Lin Jixu , Lin Qiangkang
Published: July 2021

This study aimed to comprehensively examine the efficacy of chemotherapy in T1 colon cancer patients with lymph node metastasis.

Methods

The differences in categorical variables in colon cancer patients according to lymph node status were evaluated by Pearson’s chi-square test. The Kaplan-Meier method was used to assess Cancer-specific survival (CSS) and overall survival (OS) with the log-rank test. Cox proportional hazards models were built, multivariate Cox regression analyses were performed with the hazard ratio (HR) and 95% confidence interval (CI) to identify the potential independent prognostic factors. Propensity score matching was also undertaken to adjust for treatment bias due to measured confounders.

Results

Younger age (52.2% VS. 43.0% for ≤ 65 years old, p < 0.001), female gender (50.3% VS. 46.8% for female, p < 0.001), more lymph nodes harvested (68.1% VS. 46.6% for ≥12 lymph nodes harvested, p < 0.001), Black race (13.6% VS. 12.0% for the Black race, p < 0.001), and higher tumor grade (14.2% VS. 5.6% for grade III/IV, p < 0.001) were more prone to be diagnosed with lymph node involvement. The receipt of adjuvant chemotherapy following radical surgery significantly reduced the risk of colon cancer-specific mortality by 33.9% after propensity-score matching (HR = 0.661, 95%CI = 0.476-0.917, p = 0.013).

Conclusions

Younger age, female gender, more lymph nodes harvested, Black race, and higher tumor grade were more prone to be diagnosed with lymph node involvement. The receipt of adjuvant chemotherapy following radical surgery also significantly decreased the risk of colon cancer-specific mortality by 33.9% in T1 colon cancer with lymph node involvement.
N6-Methyladenosine Regulators and Related LncRNAs Are Potential to be Prognostic Markers for Uveal Melanoma and Indicators of Tumor Microenvironment Remodeling
Authors: Liu Zhicheng , Li Shanshan , Huang Shan , Wang Tao , Liu Zhicheng
Published: July 2021

Uveal melanoma (UM) is one of the most common malignant intraocular tumors in adults. Few studies have investigated the effect of N6-methyladenosine (m6A) RNA methylation regulators and related long noncoding RNAs (lncRNAs) on the tumor microenvironment (TME) and survival time of patients with UM. Based on the transcriptome and clinical data from The Cancer Genome Atlas, we systematically identified m6A regulators. Then, we constructed an m6A regulators-based signature to predict the prognostic risk using univariate and LASSO Cox analyses. The signature was then validated by performing Kaplan-Meier, and receiver operating characteristic analyses. Through the correlation analysis, m6A regulators-related lncRNAs were identified, and they were divided into different clustering subtypes according to their expression. We further assessed differences in TME scores, the survival time of patients, and immune cell infiltration levels between different clustering subtypes. Finally, we screened out the common immune genes shared by m6A-related lncRNAs and determined their expression in different risk groups and clustering subtypes. For further validation, we used single-cell sequencing data from the GSE139829 dataset to explore the expression distribution of immune genes in the TME of UM. We constructed a prognostic risk signature representing an independent prognostic factor for UM using 3 m6A regulators. Patients in the low-risk group exhibited a more favorable prognosis and lower immune cell infiltration levels than patients in the high-risk group. Two subtypes (cluster 1/2) were identified based on m6A regulators-related lncRNAs. The TME scores, prognosis, and immune cell infiltration have a marked difference between cluster 1 and cluster 2. Additionally, 13 common immune genes shared by 5 lncRNAs were screened out. We found that these immune genes were differentially expressed in different risk groups and clustering subtypes and were widely distributed in 3 cell types of TME. In conclusion, our study demonstrated the important role of m6A regulators and related lncRNAs in TME remodeling. The signature developed using m6A regulators might serve as a promising parameter for the clinical prediction of UM.
Bullying Victimization and Life Satisfaction Among Rural Left-Behind Children in China: A Cross-Sectional Study
Authors: Yang Yide , Zheng Chanjuan , Xie Ming , Yuan Shuqian , Zeng Yuan , Zhou Meiling , Huang Shuzhen , Zhu Yulian , Ye Xiangli , Zou Zhiyong , Wang Ying , Baker Julien Steven
Published: July 2021

Objectives: This study aimed to evaluate the associations between bullying victimization and life satisfaction in primary school children and also investigate the interactive effects of left-behind status and bullying victimization on life satisfaction.

Materials and Methods: Bullying victimization was measured using the Chinese version of the revised Olweus Bully/Victim Questionnaire. Life satisfaction was assessed using the Multidimensional Students' Life Satisfaction Scale (MSLSS). Life satisfaction is composed of five domains, namely, family, school, friends, environment, and self-satisfaction. Left-behind status of rural children was defined as one or both their parents migrating to working in cities. The data were analyzed using Mann–Whitney U tests, Chi-square tests, and multivariate linear and logistic regression analyses.

Results: A total of 810 primary school children were involved, of which 8.5% reported bullying victimization, and 44.3% were left-behind children (LBC). We found that bullying victimization was negatively associated with all domains of life satisfaction (all p < 0.05). With further left-behind status-stratified analysis, we found that negative association between bullying victimization and friend satisfaction was more profound in the LBC group than in the non-LBC group [b(SE)= −0.133 (0.03) vs. −0.061 (0.026) for LBC and non-LBC, respectively, p < 0.05]. When further interaction analysis was conducted, we identified interaction effects between left-behind status and bullying victimization on friend satisfaction (pinteraction = 0.048). Similar interaction effect between bullying victimization and left-behind status on school satisfaction was also found (pinteraction = 0.004).

Conclusions: Bullying victimization was associated with low life satisfaction (including lower family, friends, school, self, and environment satisfaction). There were significant interactions between left-behind status and bullying victimization on friend satisfaction, as well as school satisfaction. Left-behind status of children may exaggerate the impact of bullying victimization on friends/school satisfaction rating.
Astragalus-Containing Chinese Herbal Medicine Combined With Chemotherapy for Cervical Cancer: A Systematic Review and Meta-Analysis
Authors: Shen Lei , Gwak Si Ra , Cui Zhen Yang , Joo Jong Cheon , Park Soo Jung
Published: July 2021

Background: Cervical cancer is the fourth most common malignant tumor among women worldwide. This study aimed to evaluate the efficacy of Astragalus-containing Chinese herbal medicine (CHM) combined with chemotherapy (CT) for the treatment of cervical cancer.

Methods: Ten electronic databases including PubMed, Cochrane Library, Embase, Korean databases, and Chinese medical databases, were systematically searched up to July 2020. All randomized controlled trials using Astragalus-containing CHM combined with CT to treat cervical cancer were included.

Results: A total of 19 trials were included in the analysis. Compared with the control group, the Astragalus-containing CHM combined with CT group showed a significantly increased tumor response (complete and partial response (CR and PR)) (risk ratio [RR] = 1.25, 95% confidence interval [CI]: 1.17–1.33, p < 0.00001) and Karnofsky performance score (KPS) (standardized mean difference [SMD] = 1.81, 95% CI: 1.46–2.17, p < 0.00001). This group also displayed remarkably reduced CT toxicity.

Conclusion: Our study suggests that Astragalus-containing CHM might be a potential option for cervical cancer to enhance the curative efficacy and reduce CT toxicity.
Incidence and Risk Factors for Antiplatelet Therapy–Related Bleeding Complications Among Elderly Patients After Coronary Stenting: A Multicenter Retrospective Observation
Authors: Qian Yanxia , Xu Bing , Qian Xiaodong , Cao Lu , Cheng Yujia , Liu Xinjian , Bai Song , Han Zhijun , Wang Junhong
Published: July 2021

Purpose: To determine the incidence and risk factors of bleeding events as well as assess the performance of the PRECISE-DAPT score in elderly patients (≥75 years) who underwent percutaneous coronary intervention (PCI) and one-year dual antiplatelet therapy (DAPT).

Methods: A total of 940 patients (≥75 years) who received PCI and one-year DAPT were retrospectively enrolled into the study. The multivariable logistic regression analysis was conducted to identify risk factors of antiplatelet-related bleeding complications. The receiver operating characteristic (ROC) curve analysis and the Delong test were performed to obtain the optimized PRECISE-DAPT score.

Results: It was observed that 89 (9.47%) patients suffered bleeding complications, while 37 (3.94%) of them had the Bleeding Academic Research Consortium (BARC, type ≥2) bleeding events. We stratified the PRECISE-DAPT score in tertiles (T1: ≤23; T2:24 to 32; T3: ≥33) and found that BARC ≥ 2 type bleeding occurred more frequently in T3 than in T1 and T2 (8.25 vs. 1.46% vs. 2.40%, p <0.05). The ROC curve analysis revealed that the PRECISE-DAPT score cutoff for BARC ≥2 type bleeding prediction was 33. In comparison with the current recommended cutoff score of 25 (AUC: 0.608, based on ROC analysis), the Delong test indicated significantly improved ability for predicting BARC ≥ 2 type bleeding events using the proposed cutoff value of 33, AUC of 0.676 (p = 0.03), and Brier Score of 0.04. The multivariable logistic regression analysis demonstrated that the PRECISE-DAPT score ≥ 33 [OR: 3.772; 95% CI (1.229, 11.578); p = 0.02] was associated with BARC ≥ 2 type bleeding event, along with a history of hemorrhagic stroke [OR: 6.806; 95% CI (1.465, 31.613); p = 0.014], peptic ulcer [OR: 3.871; 95% CI (1.378, 10.871); p = 0.01], and/or myocardial infarction [MI, OR: 3.081; 95% CI (1.140, 8.326); p = 0.027].

Conclusion: A higher PRECISE-DAPT score of 33 might be a more reasonable cutoff value for predicting BARC ≥2 type bleeding risk in CAD patients (≥75 years). In addition, the history of hemorrhagic stroke, peptic ulcer, and myocardial infarction were identified as the risk factors of BARC ≥2 type bleeding events.
The Advancing of Selenium Nanoparticles Against Infectious Diseases
Authors: Lin Wensen , Zhang Junai , Xu Jun-Fa , Pi Jiang
Published: July 2021

Infectious diseases, caused by the direct exposure of cellular or acellular pathogens, are found to be closely associated with multiple inflammation and immune responses, keeping one of the top threats to human health. As an indispensable trace element, Selenium (Se) plays important roles in antioxidant defence and redox state regulation along with a variety of specific metabolic pathways. In recent decades, with the development of novel nanotechnology, Selenium nanoparticles (Se NPs) emerged as a promising agent for biomedical uses due to their low toxicity, degradability and high bioavailability. Taking the advantages of the strong ability to trigger apoptosis or autophagy by regulating reactive oxygen species (ROS), Se NPs have been widely used for direct anticancer treatments and pathogen killing/clearance in host cells. With excellent stability and drug encapsulation capacity, Se NPs are now serving as a kind of powerful nano-carriers for anti-cancer, anti-inflammation and anti-infection treatments. Notably, Se NPs are also found to play critical roles in immunity regulations, such as macrophage and T effector cell activation, which thus provides new possibilities to achieve novel nano-immune synergetic strategy for anti-cancer and anti-infection therapies. In this review, we summarized the progress of preparation methods for Se NPs, followed by the advances of their biological functions and mechanisms for biomedical uses, especially in the field of anti-infection treatments. Moreover, we further provide some prospects of Se NPs in anti-infectious diseases, which would be helpful for facilitating their future research progress for anti-infection therapy.
Isoforskolin Alleviates AECOPD by Improving Pulmonary Function and Attenuating Inflammation Which Involves Downregulation of Th17/IL-17A and NF-κB/NLRP3
Authors: Xiao Chuang , Cheng Sha , Li Runfeng , Wang Yutao , Zeng Deyou , Jiang Haiming , Liang Yaping , Huang Rong , Pan Hanxiao , Wu Xiao , Fang Yan , Chen Chen , Li Xian , Zhang Rongping , Wang Xinhua , Yang Zifeng , Yang Weimin
Published: July 2021

Chronic obstructive pulmonary disease (COPD), a major cause of morbidity and mortality worldwide, is widely considered to be related to cigarette smoke (CS), and viral infections trigger acute exacerbation of COPD (AECOPD). Isoforskolin (ISOF) is a bioactive component from the plant Coleus forskohlii, native to Yunnan in China. It has been demonstrated that ISOF has anti-inflammatory effect on acute lung injury animal models. In the present study, we investigated the efficacy and mechanism of ISOF for the prevention and treatment of AECOPD. Mice were exposed to CS for 18 weeks and then infected with influenza virus A/Puerto Rico/8/34 (H1N1). ISOF (0.5, 2 mg/kg) was intragastrically administered once a day after 8 weeks of exposure to cigarette smoke when the body weight and lung function of model mice declined significantly. The viral load, pulmonary function, lung morphology, Th17 cells, and inflammatory cytokines in lung tissues were evaluated. The expression of nuclear factor κB (NF-κB) and NOD-like receptor pyrin domain–containing protein 3 (NLRP3) inflammasome pathways were detected. The results showed that ISOF treatment reduced the viral load in the lung homogenate, decreased the lung index of model mice, and lung pathological injuries were alleviated. ISOF also improved the pulmonary function with increased FEV0.1/FVC and decreased Rn and Rrs. The levels of inflammatory mediators (TNF-α, IL-1β, IL-6, IL-17A, MCP-1, MIG, IP-10, and CRP) in the lung homogenate were reduced after ISOF treatment. ISOF decreased the proportion of Th17 cells in the lung tissues by the flow cytometry test, and the protein expression levels of RORγt and p-STAT3 were also decreased. Furthermore, ISOF significantly inhibited the activation of NF-κB signaling and NLRP3 inflammasome in the lung tissues of model mice. In conclusion, ISOF alleviates AECOPD by improving pulmonary function and attenuating inflammation via the downregulation of proinflammatory cytokines, Th17/IL-17 A, and NF-κB/NLRP3 pathways.
New Insight Into the Cardioprotective Effects of Allium ursinum L. Extract Against Myocardial Ischemia-Reperfusion Injury
Authors: Rankovic Marina , Krivokapic Milos , Bradic Jovana , Petkovic Anica , Zivkovic Vladimir , Sretenovic Jasmina , Jeremic Nevena , Bolevich Sergey , Kartashova Maria , Jeremic Jovana , Bolevich Stefani , Jakovljevic Vladimir , Tomovic Marina
Published: July 2021

This study aimed to estimate the effects of increasing doses of Allium ursinum methanol extract on cardiac ischemia/reperfusion injury (I/R) with a special emphasis on the role of oxidative stress. Fifty rats were randomly divided into five groups (10 animals per group) depending on the applied treatment as follows: sham, rats who drank only tap water for 28 days and hearts were retrogradely perfused for 80 min without I/R injury, I/R, rats who drank only tap water for 28 days and hearts were exposed to ex vivo I/R injury and rats who consumed increasing doses of A. ursinum 125, 250, and 500 mg/kg for 28 days before I/R injury. Hearts from all rats were isolated and retrogradely perfused according to the Langendorff technique. Parameters of oxidative stress were spectrophotometrically measured in blood, coronary venous effluent, and heart tissue samples. Intake of wild garlic extract for 28 days significantly contributed to the recovery of cardiac function, which was reflected through preserved cardiac contractility, systolic function, and coronary vasodilatory response after ischemia. Also, wild garlic extract showed the potential to modulate the systemic redox balance and stood out as a powerful antioxidant. The highest dose led to the most efficient decrease in cardiac oxidative stress and improve recovery of myocardial function after I/R injury. We might conclude that wild garlic possesses a significant role in cardioprotection and strong antioxidant activity, which implicates the possibility of its use alone in the prevention or as adjuvant antioxidant therapy in cardiovascular diseases (CVD).
Post-Traumatic Distress and Burnout Among Chinese School Teachers: The Mediating Role of Forgiveness
Authors: Wang Yabing , Chung Man Cheung , Fang Siqi
Published: July 2021

Purpose

Teachers’ mental health is concerning due to high stress at work. Its association with job-related stressors has been well-documented. Little is known; however, about how traumatic life events and trauma reactions might contribute to their psychological distress. This paper is to explore whether Post-traumatic Stress Disorder (PTSD) following past traumatic event would predict burnout and psychiatric co-morbidity among Chinese k-12 school teachers and whether this prediction would be mediated by forgiveness after controlling for work-related factors.

Methods

Two hundred and seventy-nine Chinese teachers (F = 223, M = 56) from primary and secondary schools completed demographic information, Post-traumatic Stress Disorder Checklist for DSM-5 (PCL-5), Heartland Forgiveness Scale (HFS), General Health Questionnaire-28 (GHQ-28), Maslach Burnout Inventory-Educator’s Survey (MBI-ES), and a series of measures assessing work-related factors.

Results

Structured equation modeling (SEM) showed that after controlling for work-related factors, PTSD following past trauma was positively associated with burnout and general psychological problems but negatively associated with levels of forgiveness. Forgiveness carried the impact of PTSD onto burnout rather than general psychological distress.

Conclusion

To conclude, regardless of the level of stress experienced from working in school, primary and secondary teachers with PTSD from past trauma found it more difficult forgiving which in turn could affect their levels of burnout.
But What’s Your Partner Up to? Associations Between Relationship Quality and Pornography Use Depend on Contextual Patterns of Use Within the Couple
Authors: Kohut Taylor , Dobson Kiersten A. , Balzarini Rhonda N. , Rogge Ronald D. , Shaw Amanda M. , McNulty James K. , Russell V. Michelle , Fisher William A. , Campbell Lorne
Published: July 2021

It is commonly assumed that exposure to pornography harms relationships because pornography changes the way that individuals think, feel, and behave in problematic ways. In the current research, we contribute to a small but growing body of work that challenges this assumption by carefully scrutinizing the relational context of pornography use. In contrast to dominant theoretical explanations in this field, we argue that at least some of the apparent negative “impacts” of pornography use on relationship quality may reflect partner dissimilarity in pornography use behavior rather than the consequences of exposure to such materials. Moreover, we further examine a particular type of pornography use – shared use with a partner – which previous evidence suggests may be positively associated with relationship quality. To this end, we sought to test whether dyadic patterns of pornography use, and related attributes, were associated with sexual and relationship satisfaction in two cross-sectional (N1 = 200; N3 = 207) and two longitudinal (N2 = 77; N4 = 277) samples of heterosexual couples. Across these samples, we found consistent evidence that partners who watch pornography together report higher relationship and sexual satisfaction than partners who do not, and notably, this association was not moderated by gender. Independent of this association, we also found evidence of a similarity-dissimilarity effect, such that the solitary pornography use of one partner was negatively associated with their own relationship and sexual satisfaction, but only in cases where their romantic partners used little or no pornography alone. Further consideration of several correlates of pornography use established comparable patterns of results for dissimilarity in attitudes toward pornography, erotophobia-erotophilia, sexual preferences, and sex drive. Importantly, only dissimilarity in sex drive statistically accounted for dissimilarity in solitary pornography use, suggesting that differences in sex drive may be implicated in the associations between pornography use and relationship quality. These findings demonstrate that links between pornography use and relationship health are partially a function of different dyadic patterns of pornography use within couples and do not always suggest relational harm.
High-Resolution Comparative Genomics of Salmonella Kentucky Aids Source Tracing and Detection of ST198 and ST152 Lineage-Specific Mutations
Authors: Soltys Rachel C. , Sakomoto Carson K. , Oltean Hanna N. , Guard Jean , Haley Bradd J. , Shah Devendra H.
Published: July 2021

Non-typhoidal Salmonella (NTS) is a major cause of foodborne illness globally. Salmonella Kentucky is a polyphyletic NTS serovar comprised of two predominant multilocus sequence types (STs): ST152 and ST198. Epidemiological studies have revealed that ST152 is most prevalent in US poultry whereas ST198 is more prevalent in international poultry. Interestingly, ST152 is sporadically associated with human illness, whereas ST198 is more commonly associated with human disease. The goal of this study was to develop a better understanding of the epidemiology of ST198 and ST152 in WA State. We compared the antimicrobial resistance phenotypes and genetic relationship, using pulsed-field gel electrophoresis, of 26 clinical strains of S. Kentucky isolated in Washington State between 2004 and 2014, and 140 poultry-associated strains of S. Kentucky mostly recovered from the northwestern USA between 2004 and 2014. We also sequenced whole genomes of representative human clinical and poultry isolates from the northwestern USA. Genome sequences of these isolates were compared with a global database of S. Kentucky genomes representing 400 ST198 and 50 ST152 strains. The results of the phenotypic, genotypic, and case report data on food consumption and travel show that human infections caused by fluoroquinolone-resistant (FluR) S. Kentucky ST198 in WA State originated from outside of North America. In contrast, fluoroquinolone-susceptible (FluS) S. Kentucky ST198 and S. Kentucky ST152 infection have a likely domestic origin, with domestic cattle and poultry being the potential sources. We also identified lineage-specific non-synonymous single nucleotide polymorphisms (SNPs) that distinguish ST198 and ST152. These SNPs may provide good targets for further investigations on lineage-specific traits such as variation in virulence, metabolic adaptation to different environments, and potential for the development of intervention strategies to improve the safety of food.
Intersubjetividad y estructura narrativa en la terapia familiar
Authors: Mook Berta
Published: July 2021

Los terapeutas familiares tienden a aceptar el modelo sistémico como una realidad fundamental y incuestionable, olvidando que es sólo una metáfora útil para observar la interrelación de la familia. En el proceso, pasan de largo otra realidad, a saber, que los miembros de la familia también son individuos separables con sus propios centros de iniciativa y responsabilidad. Como resultado, los terapeutas familiares se centran en las relaciones pero tienden a desatender la experiencia personal. En este artículo, se intenta aportar una nueva contribución a la teoría de la terapia familiar, desde la perspectiva epistemológica y hermenéutica, sugiriendo dos proposiciones fundamentales: primero, que la familia humana es co-constituida como una comunidad inter subjetiva; y segundo, que las experiencias y acciones de los miembros de la familia son intrínsecamente estructuradas de una forma análoga a las estructuras narrativas.
ALBERT over Match-LSTM Network for Intelligent Questions Classification in Chinese
Authors: Wang Xiaomin , Wang Haoriqin , Zhao Guocheng , Liu Zhichao , Wu Huarui
Published: July 2021

This paper introduces a series of experiments with an ALBERT over match-LSTM network on the top of pre-trained word vectors, for accurate classification of intelligent question answering and thus the guarantee of precise information service. To improve the performance of data classification, a short text classification method based on an ALBERT and match-LSTM model was proposed to overcome the limitations of the classification process, such as few vocabularies, sparse features, large amount of data, lots of noise and poor normalization. In the model, Jieba word segmentation tools and agricultural dictionary were selected to text segmentation, GloVe algorithm was then adopted to expand the text characteristic and weighted word vector according to the text of key vector, bi-directional gated recurrent unit was applied to catch the context feature information and multi-convolutional neural networks were finally established to gain local multidimensional characteristics of text. Batch normalization, Dropout, Global Average Pooling and Global Max Pooling were utilized to solve overfitting problem. The results showed that the model could classify questions accurately, with a precision of 96.8%. Compared with other classification models, such as multi-SVM model and CNN model, ALBERT+match-LSTM had obvious advantages in classification performance in intelligent Agri-tech information service.
Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives
Authors: Singh Chandra Mohan , Singh Poornima , Tiwari Chandrakant , Purwar Shalini , Kumar Mukul , Pratap Aditya , Singh Smita , Chugh Vishal , Mishra Awdhesh Kumar
Published: July 2021

Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.
Effect of Wet Aging on Color Stability, Tenderness, and Sensory Attributes of Longissimus lumborum and Gluteus medius Muscles from Water Buffalo Bulls
Authors: Jaspal Muhammad Hayat , Badar Iftikhar Hussain , Amjad Osama Bin , Yar Muhammad Kashif , Ijaz Muawuz , Manzoor Adeel , Nasir Jamal , Asghar Bilal , Ali Sher , Nauman Kashif , Rahman Abdur , Wara Um Ul
Published: July 2021

The present study aimed to investigate the effect of wet aging on meat quality characteristics of Longissimus lumborum (LL) and Gluteus medius (GM) muscles of buffalo bulls. Meat samples from six aging periods, i.e., 0 day (d) = control, 7 d, 14 d, 21 d, 28 d, and 35 d, were evaluated for pH, color, metmyoglobin content (MetMb%), cooking loss, water holding capacity (WHC), myofibrillar fragmentation index (MFI), Warner–Bratzler shear force (WBSF), and sensory evaluation. The pH, instrumental color redness (a *), yellowness (b *), chroma (C *), and MetMb% values were increased, while the lightness (L *) and hue angle (h *) values showed non-significant (p > 0.05) differences in both LL and GM muscles in all aging periods. The cooking loss increased while WHC decreased till 35 days of aging. MFI values significantly (p < 0.05) increased, while WBSF values decreased; in addition, sensory characteristics were improved with the increase in the aging period. Overall, the color, tenderness, and sensory characteristics were improved in LL and GM muscles until 28 and 21 days of aging, respectively. Based on the evaluated meat characteristics, 28 days of aging is required to improve the meat quality characteristics of LL, whereas 21 days of aging is suitable for GM muscle.
Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro
Authors: Kihal Abdelhacib , Rodríguez-Prado María Ercilda , Cristofol Carles , Calsamiglia Sergio
Published: July 2021

The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of vitamins A, D and E in the incubation conditions. In Experiment 2, the main factors were the MTB (bentonite, clinoptilolite, sepiolite, montmorillonite, active carbon and yeast cell walls), vitamins (A, D and E) and incubation type (vitamins incubated separately or together). The recovery was high for vitamin D (83%) and E (93%), but low for vitamin A (23%), for which no further analyses were conducted. When incubated separately, vitamin D was only adsorbed by yeast cell wall (20.2%). Vitamin E adsorption was highest with bentonite (54.5%) and montmorillonite (46.3%) and lowest with sepiolite (16.6%) and active carbon (18.5%). When incubated together, vitamin D was not adsorbed by any MTB. Vitamin E adsorption was highest in bentonite (61.8%) and montmorillonite (50.7%) and lowest in sepiolite (15.4%). Results indicate that the bioavailability of vitamin E, but not that of vitamin D, may be reduced in the presence of MTBs.
Dead-End (dnd) Gene Cloning and Gonad-Specific Expression Pattern in Starry Flounder (Platichthys stellatus)
Authors: Yoon Ji-Hye , Cho Youn-Su , Lee Hyo-Bin , Park Jung-Yeol , Lim Han-Kyu
Published: July 2021

dnd is a germline-specific maternal RNA expressed in various vertebrate classes, which encodes an RNA-binding protein that is essential for PGC migration. The purpose of this study is fundamental research about starry flounder dnd gene for germ cell marker development. In this study, we cloned and analyzed the expression levels of Platichthys stellatus dead end (psdnd) in various tissues and embryonic stages. The psdnd gene was isolated from starry flounder ovaries, cloned into a pGEM-t vector, and sequenced. Full-length of psdnd cDNA was 1495 bp long, encoding 395 amino acids. psdnd expression levels were investigated by real-time polymerase chain reaction (qRT-PCR) in various tissues and embryo developmental stages. psdnd transcripts were detected in the testes and ovaries, but not in somatic tissues. Embryonic psdnd expression levels were higher during early embryo development stages than during late embryogenesis; psdnd expression was highest at the 1 cell stage, then gradually decreased throughout the subsequent developmental stages. The spatial expression pattern was analyzed by whole-mount in situ hybridization (WISH). The psdnd transcripts migration pattern was similar with zebrafish (Danio rerio). Our results suggest that psdnd may function as a germ cell-specific marker.
High β-Lactam and Quinolone Resistance of Enterobacteriaceae from the Respiratory Tract of Sheep and Goat with Respiratory Disease
Authors: Khalifa Hazim O. , Oreiby Atef , Abd El-Hafeez Amer Ali , Abd El Latif Amira , Okanda Takashi , Kato Yasuyuki , Matsumoto Tetsuya
Published: July 2021

During the last decade’s increase of antimicrobial resistance (AMR) in animals, animal-human transmission has become a major threat. Therefore, the present study aimed to evaluate the genetic basis of AMR in Gram-negative bacteria recovered from sheep and goats with respiratory disease. Nasal and ocular swabs were collected from 69 diseased animals, and 76 Gram-negative bacterial isolates were identified from 59 animals. All isolates were checked phenotypically for resistance and genotypically for different resistance mechanisms, including β-lactam, quinolone, and aminoglycoside resistance. Our results demonstrated that 9.2% (95% CI 4.5–17.8%) of the isolates were multidrug-resistant, with high resistance rates to β-lactams and quinolones, and 11.8% (95% CI 6.4–21%) and 6.6% (95% CI 2.8–14.5%) of the isolates were phenotypically positive for AmpC and ESBL, respectively. Genotypically, blaTEM was the most identified β-lactamase encoding gene in 29% (95% CI 20–40%) of the isolates, followed by blaSHV (14.5%, 95% CI 8.3–24.1%) and blaCTX-M (4%, 95% CI 1.4–11%). Furthermore, 7.9% (95% CI 3.7–16.2%) of the isolates harbored plasmid-mediated quinolone resistance gene qnrS. Our study revealed for the first time to our knowledge high β-lactam and quinolone resistance associated with the bacteria recovered from sheep and one goat with respiratory disease. Furthermore, different antimicrobial resistant determinants were identified for the first time from animals in Africa, such as blaLEN-13/55, blaTEM-176 and blaTEM-198/214. This study highlights the potential role of sheep and goats in disseminating AMR determinants and/or resistant bacteria to humans. The study regenerates interest for the development of a One Health approach to combat this formidable problem.
Give Them a Toy or Increase Time out of Kennel at Lawn Areas: What Is the Influence of These Interventions on Police Dogs’ Welfare?
Authors: Nogueira Letícia Bicudo , Palme Rupert , Mendonça-Furtado Olívia
Published: July 2021

This work was aimed at identifying the effectiveness of two interventions applied to a group of eight kennelled police dogs. Interventions consisted of access to a lawn area (350 m2) and “toy” (a 30 cm jute rag roll, hanging from the kennel ceiling), both available for 15 min a day, for four days in a row. We collected behavioural data and faecal samples for cortisol metabolites evaluation before, during and after interventions. Faecal cortisol metabolites levels were significantly reduced (Friedman, X2(3) = 12.450; p = 0.006) during the second round of intervention, regardless of the type of intervention, indicating that the interventions can have a beneficial cumulative effect. Regarding the frequency of stereotyped behaviour, cluster analysis identified two groups of individuals: (1) high-stereotyping individuals (n = 3) that had a tendency to reduce stereotyping behaviours in the lawn intervention when compared to toy intervention (Friedman, X2(3) = 2.530; p = 0.068), and (2) low-stereotyping animals (n = 5) that did not present significant behavioural changes during the experiment. The variety of behavioural and endocrine parameters evaluated highlights the need to account for the individual in behaviour and welfare research. Overall, our results suggest that even simple environmental enrichment can be an effective method to mitigate behaviour and physiological signs of stress.
Protective Effect of Baicalin against Clostridioides difficile Infection in Mice
Authors: Pellissery Abraham Joseph , Vinayamohan Poonam Gopika , Kuttappan Deepa Ashwarya , Mishra Neha , Fragomeni Breno de Oliveira , Maas Kendra , Mooyottu Shankumar , Venkitanarayanan Kumar
Published: July 2021

This study investigated the prophylactic and therapeutic efficacies of baicalin (BC), a plant-derived flavone glycoside, in reducing the severity of Clostridioides difficile infection (CDI) in a mouse model. In the prophylactic trial, C57BL/6 mice were provided with BC (0, 11, and 22 mg/L in drinking water) from 12 days before C. difficile challenge through the end of the experiment, whereas BC administration started day 1 post challenge in the therapeutic trial. Both challenge and control groups were infected with 106 CFU/mL of hypervirulent C. difficile BAA 1803 spores or sterile PBS, and the clinical and diarrheal scores were recorded for 10 days post challenge. On day 2 post challenge, fecal and tissue samples were collected from mice prophylactically administered with BC for microbiome and histopathologic analysis. Both prophylactic and therapeutic supplementation of BC significantly reduced the severity of colonic lesions and improved CDI clinical progression and outcome compared with control (p < 0.05). Microbiome analysis revealed a significant increase in Gammaproteobacteria and reduction in the abundance of protective microbiota (Firmicutes) in antibiotic-treated and C. difficile-infected mice compared with controls (p < 0.05). However, baicalin supplementation favorably altered the microbiome composition, as revealed by an increased abundance in beneficial bacteria, especially Lachnospiraceae and Akkermansia. Our results warrant follow-up investigations on the use of BC as an adjunct to antibiotic therapy to control gut dysbiosis and reduce C. difficile infection in humans.
Generation of an HBIM Library regarding a Palace of the 19th Century in Lisbon
Authors: Sampaio Alcínia Zita , Pinto António Mendes , Gomes Augusto Martins , Sanchez-Lite Alberto
Published: July 2021

The growing interest within the construction industry in the preservation, rehabilitation, and conversion of heritage-value buildings has led to the implementation of Historic Building Information Modelling (HBIM) across all sectors. The rigorously accurate representation of old construction solutions for roofs, floors, and walls, and of the decorative details and finishes, commonly used in historic buildings of patrimonial value, requires specific geometric modelling processes in order to generate relevant libraries of parametric objects. This study addresses the generation of parametric families of representative architectural geometry in the context of the conversion of a building of patrimonial value. A collection of historical information and a detailed inspection in situ, recorded by means of digital images and sketches with annotative dimensions of the architectural forms, were first carried out to support the accurate representation of the building. Several families of objects were generated, namely, guillotine windows, glazed doors, ornamental stonework, staircases, and handrails, thus creating a reference library for use in further similar building projects. The study improves the potential of the BIM process for its application to buildings with distinctive architecture in the context of the preservation or conversion of heritage buildings.
Numerical Parametric Study on the Effectiveness of the Contact-Point Response of a Stationary Vehicle for Bridge Health Monitoring
Authors: Hashlamon Ibrahim , Nikbakht Ehsan , Topa Ameen , Elhattab Ahmed
Published: July 2021

Indirect bridge health monitoring is conducted by running an instrumented vehicle over a bridge, where the vehicle serves as a source of excitation and as a signal receiver; however, it is also important to investigate the response of the instrumented vehicle while it is in a stationary position while the bridge is excited by other source of excitation. In this paper, a numerical model of a stationary vehicle parked on a bridge excited by another moving vehicle is developed. Both stationary and moving vehicles are modeled as spring–mass single-degree-of-freedom systems. The bridges are simply supported and are modeled as 1D beam elements. It is known that the stationary vehicle response is different from the true bridge response at the same location. This paper investigates the effectiveness of contact-point response in reflecting the true response of the bridge. The stationary vehicle response is obtained from the numerical model, and its contact-point response is calculated by MATLAB. The contact-point response of the stationary vehicle is investigated under various conditions. These conditions include different vehicle frequencies, damped and undamped conditions, different locations of the stationary vehicle, road roughness effects, different moving vehicle speeds and masses, and a longer span for the bridge. In the time domain, the discrepancy of the stationary vehicle response with the true bridge response is clear, while the contact-point response agrees well with the true bridge response. The contact-point response could detect the first, second, and third modes of frequency clearly, unlike the stationary vehicle response spectra.
Determining the Prognostic Validity of the Unilateral Horizontal Cyclic Jumps Test in Sprint Performance
Authors: Babić Vesna , Milinović Ivan , Čule Marko , Dolenec Aleš
Published: July 2021

In sports practice, motor tests are commonly used to predict success in specific segments of sprint running, as well as post-injury tests in rehabilitation. The purpose of this analytical cross-sectional study was to determine the prognostic validity of the unilateral horizontal cyclic jumps for a 20 m (UHCJ20m) test on sprint running success. A sample of 118 kinesiology students aged 20.5 ± 1.2 years with an average height of 179.7 ± 6.4 cm and a body mass of 75.6 ± 7.3 kg was used to determine the correlation between the results of the UHCJ20m test and the results of the 20 m sprint start run (MRLS20m), the 20 m sprint flying start run (MRFS20m), and the 100 m run (M100m). The results showed a moderate correlation in all tests (MRLS20m (R = 0.49), MRFS20m (R = 0.53), and M100m (R = 0.38)) with UHCJ20m. In addition to the final result, the multiple regression analysis showed a significant moderate correlation between the kinematic parameters of the UHCJ20m test and the results in the MRLS20m (R = 0.38), MRFS20m (R = 0.49), and M100m (R = 0.37) tests. The stride length (SL) and the contact time (CT) of the UHCJ20m test were statistically significant predictors for the 100 m sprint, the number of steps for MRLS20m, and the SL and the CT for MRFS20m sprint success. Unilateral horizontal cyclic jumps are a significant predictor of success in sprint running, especially for the maximal speed running segment.
Two-Dimensional Shape Analysis of Complex Geometry Based on Photogrammetric Models of Iconostases
Authors: Đurić Isidora , Obradović Ratko , Vasiljević Ivana , Ralević Nebojša , Stojaković Vesna
Published: July 2021

Three-dimensional digitization technologies have been proved as reliable methods for detailed and accurate spatial data collection from existing cultural heritage. In addition, the point segmentation techniques are particularly relevant for contour detection and classification of the unstructured point cloud. This paper describes an approach to obtain 2D CAD-like visualizations of complex geometry from photogrammetric models so that the detected contours of particular object elements can be used for 2D shape analysis. The work process uses the point clouds derived from photogrammetric models to create the plane visualization of the object’s geometry by segmenting points based on the verticality geometric feature. The research presented is on the case studies of iconostases as the specific art and architectural elements of the Christian Orthodox church that can be appreciated only in situ. To determine relations between the characteristics of the particular shapes and the iconostases’ style origins, the mathematical method of shape analysis was applied. This study aims to numerically describe the stylistic characteristics of the shapes of the main parts of the iconostasis concerning the artistic period to which it belongs to. The concept was based on the consideration of global shape descriptors and associated shape measurements which were used to analyze and classify the stylistic characteristics of the iconostases. The methodology was applied to the representative examples of three iconostases from the Baroque and Classicism art movements. The results illustrated that the proposed methods and techniques, with certain improvements, could be helpful for CAD visualization and shape analysis of complex geometry.
Influence of Far Infrared TiO2 and Multi-Walled Carbon Nanotubes Coatings on the Performance of a Hot Water Heater
Authors: Teng Tun-Ping , Yu Shang-Pang , Lue Yeou-Feng , Xie Qi-Lin , Hsieh Hsiang-Kai , Huang Chia-Cing
Published: July 2021

This study selects titanium dioxide (TiO2) and multi-walled carbon nanotubes (MWCNTs) as far-infrared materials (FIRMs), and further adds water-based acrylic coatings to prepare far-infrared coatings (FIRCs). FIRCs are uniformly coated on #304 stainless steel sheets to make the test samples, which are then installed between the shell and insulation material of the hot water heater to measure the influences of various FIRCs on the performance of the hot water heater. The research results show no significant difference in the heating rate or heat insulation performance of the hot water heater with or without FIRCs coating. However, the uniformity of the water temperatures of the test samples is significantly improved with FIRCs. Considering that the uniformity of water temperature will inhibit the heating rate and heat insulation performance of the hot water heater, TiO2-FIRC should provide better performance improvement when applied to the hot water heater in this study. The application of TiO2-FIRC to large-scale hot water heaters with a high aspect ratio will effectively improve the quality of hot water supply in the future.
Retina-like Imaging and Its Applications: A Brief Review
Authors: Hao Qun , Tao Yu , Cao Jie , Tang Mingyuan , Cheng Yang , Zhou Dong , Ning Yaqian , Bao Chun , Cui Huan
Published: July 2021

The properties of the human eye retina, including space-variant resolution and gaze characters, provide many advantages for numerous applications that simultaneously require a large field of view, high resolution, and real-time performance. Therefore, retina-like mechanisms and sensors have received considerable attention in recent years. This paper provides a review of state-of-the-art retina-like imaging techniques and applications. First, we introduce the principle and implementing methods, including software and hardware, and describe the comparisons between them. Then, we present typical applications combined with retina-like imaging, including three-dimensional acquisition and reconstruction, target tracking, deep learning, and ghost imaging. Finally, the challenges and outlook are discussed to further study for practical use. The results are beneficial for better understanding retina-like imaging.