bluuebunny/crossref_metadata_2025 huggingface dataset:
LINC00525 promotes tumour growth and epithelial‐mesenchymal transition as an oncogene in oral squamous cell carcinoma
Authors: Du Juan , Su Wenjing , Li Xiaoguang , Zu Tingjian , Bai Jinbo , Zhang Weidong , Zhou Wei
Published: May 2023

Objective

Oral squamous cell carcinoma (OSCC) is the most common malignant tumour in the oral cavity. OSCC is aggressive and prone to metastasis; it is associated with high mortality and short survival. In this study, we investigated the function of the long non‐coding RNA LINC00525 in OSCC progression and the molecular mechanisms through in vitro and in vivo experiments.

Materials and Methods

CCK8 assay was used to detect the effect of LINC00525 on cell viability; transwell migration and invasion assays and scratch assay were used to examine the role of LINC00525 in cell migration and invasion. Flow cytometry, RT‐PCR and western blot were used to detect apoptosis indexes. Tumorigenic effects were investigated using mouse xenograft tumour models.

Results

LINC00525 was associated with OSCC survival and prognosis. LINC00525 knockdown decreased cell viability and epithelial‐mesenchymal transition (EMT) properties and increased apoptosis and also shortened the cell cycle of OSCC cells in vitro. The downregulation of LINC00525 reduced the growth of OSCC tumour in vivo. LINC00525 can regulate OSCC cells via the apoptotic signalling pathway.

Conclusion

Our results indicate that LINC00525 exhibits oncogenic functions in OSCC. LINC00525 may be a new promising and potential target for the treatment of OSCC.
Detection of Common Anatomical Landmarks and Vertical Trajectories for Freehand Pedicle Screw Placement
Authors: Wang Shunmin , Zhang Weihang , Sun Jingchuan , Wang Yuan , Fan Jianping , Yu Yaping , Zhao Feng , Gao Jie , Shi Jiangang , Guo Yongfei
Published: May 2023

Objective

It is clinically important for pedicle screws to be placed quickly and accurately. Misplacement of pedicle screws results in various complications. However, the incidence of complications varies greatly due to the different professional titles of physicians and surgical experience. Therefore, physicians must minimize pedicle screw dislocation. This study aims to compare the three nail placement methods in this study, and explore which method is the best for determining the anatomical landmarks and vertical trajectories.

Methods

This study involved 70 patients with moderate idiopathic scoliosis who had undergone deformity correction surgery between 2018 and 2021. Two spine surgeons used three techniques (preoperative computed tomography scan [CTS], visual inspection‐X‐freehand [XFH], and intraoperative detection [ID] of anatomical landmarks) to locate pedicle screws. The techniques used include visual inspection for 287 screws in 21 patients, preoperative planning for 346 screws in 26 patients, and intraoperative probing for 309 screws in 23 patients. Observers assessed screw conditions based on intraoperative CT scans (Grade A, B, C, D).

Results

There were no significant differences between the three groups in terms of age, sex, and degree of deformity. We found that 68.64% of screws in the XFH group, 67.63% in the CTS group, and 77.99% in the ID group were placed within the pedicle margins (grade A). On the other hand, 6.27% of screws in the XFH group, 4.33% in the CTS group, and 6.15% in the ID group were considered misplaced (grades C and D). The results show that the total amount of upper thoracic pedicle screws was fewer, meanwhile their placement accuracy was lower. The three methods used in this study had similar accuracy in intermediate physicians (P > 0.05). Compared with intermediate physicians, the placement accuracy of three techniques in senior physicians was higher. The intraoperative detection group was better than the other two groups in the good rate and accuracy of nail placement (P < 0.05).

Conclusion

Intraoperative common anatomical landmarks and vertical trajectories were beneficial to patients with moderate idiopathic scoliosis undergoing surgery. It is an optimal method for clinical application.
Leaf anatomy explains the strength of C4 activity within the grass species Alloteropsis semialata
Authors: Alenazi Ahmed S. , Bianconi Matheus E. , Middlemiss Ella , Milenkovic Vanja , Curran Emma V. , Sotelo Graciela , Lundgren Marjorie R. , Nyirenda Florence , Pereira Lara , Christin Pascal‐Antoine , Dunning Luke T. , Osborne Colin P.
Published: May 2023

C4 photosynthesis results from anatomical and biochemical characteristics that together concentrate CO2 around ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco), increasing productivity in warm conditions. This complex trait evolved through the gradual accumulation of components, and particular species possess only some of these, resulting in weak C4 activity. The consequences of adding C4 components have been modelled and investigated through comparative approaches, but the intraspecific dynamics responsible for strengthening the C4 pathway remain largely unexplored. Here, we evaluate the link between anatomical variation and C4 activity, focusing on populations of the photosynthetically diverse grass Alloteropsis semialata that fix various proportions of carbon via the C4 cycle. The carbon isotope ratios in these populations range from values typical of C3 to those typical of C4 plants. This variation is statistically explained by a combination of leaf anatomical traits linked to the preponderance of bundle sheath tissue. We hypothesize that increased investment in bundle sheath boosts the strength of the intercellular C4 pump and shifts the balance of carbon acquisition towards the C4 cycle. Carbon isotope ratios indicating a stronger C4 pathway are associated with warmer, drier environments, suggesting that incremental anatomical alterations can lead to the emergence of C4 physiology during local adaptation within metapopulations.
Revisiting the policy implications of COVID‐19, asylum seekers, and migrants on the Mexico–U.S. border: Creating (and maintaining) states of exception in the Trump and Biden administrations
Authors: Garrett Terence M. , Sementelli Arthur J.
Published: May 2023

Public policy choices continue to bring dramatic changes to migration practices in the era of the coronavirus in the United States. In this article, we argue that the COVID‐19 pandemic facilitated the creation and maintenance of states of exception while continuing to destabilize practices at the Mexico–U.S. border through the politics of fear. Specifically, the Migrant Protection Protocols (MPP), Zero Tolerance Policy (ZTP), COVID‐19 CAPIO, Asylum Cooperative Agreements (ACA), and Title 42 used an arcane section of U.S. law to immediately expel asylum seekers and refugees. We show that these policies highlight the formation and maintenance of states of exception consistent with the work of Agamben. We further discuss how the politics of fear can reinforce hegemonic narratives targeting asylum seekers while shaping political agendas that lean toward a specific brand of nationalism using public health as a context. The U.S. government under the Trump administration—and the Biden administration to a lesser, yet continuous, extent—constructed these policies aimed primarily at refugees and asylum seekers from El Salvador, Guatemala, Honduras, and Mexico thereby violating laws and international treaty obligations.

Related Articles

Duman, Yoav H. 2014. “Reducing the Fog? Immigrant Regularization and the State.” Politics & Policy 42(2): 187–220. https://doi.org/10.1111/polp.12065.

Garrett, Terence Michael. 2020. “The Security Apparatus, Federal Magistrate Courts, and Detention Centers as Simulacra: The Effects of Trump's Zero Tolerance Policy on Migrants and Refugees in the Rio Grande Valley.” Politics & Policy 48(2): 372–95. https://doi.org/10.1111/polp.12348.

Maggio, James. 2007. “The Presidential Rhetoric of Terror: The (Re)Creation of Reality Immediately after 9/11.” Politics & Policy 35(4): 819–35. https://doi.org/10.1111/j.1747‐1346.2007.00085.x.
Negotiating the Gemeinschaft/Gesellschaft Dichotomy: Appalachian Medical Student Perceptions of Practice☆
Authors: Hedrick Jason S. , McHenry‐Sorber Erin
Published: May 2023

This study investigated motivations for Appalachian medical students to stay or leave the region weighing postgraduation options. Semi‐structured interviews were employed with final year medical students. Transcripts were open‐coded and analyzed using the theoretical concept of Gemeinschaft/Gesellschaft. Participants were in continuous negotiation between notions of Gemeinschaft and Gesellschaft in their decisions to stay or leave rural Appalachian communities. Students navigated multiple tensions in their decisions to stay or leave, including: (1) geographic isolation versus place identity and (2) community responsibility versus individual opportunity. Utilization of Gemeinschaft/Gesellschaft provides a novel contribution to the literature on decisions to stay or leave as the majority of participants hedged in their decision‐making regarding future practice location. These students tended to employ a Gesellschaft rationale to stay and a Gemeinschaft rationale to leave, expressing complicated ideas about community and individual opportunity.
Removing hepatitis C antibody testing for Australian blood donations: A cost‐effectiveness analysis
Authors: Cheng Qinglu , Hoad Veronica C. , Roy Choudhury Avijoy , Seed Clive R. , Bentley Peter , Shih Sophy T. F. , Kwon Jisoo A. , Gray Richard T. , Wiseman Virginia
Published: May 2023

Background and Objectives

The risk of transfusion‐transmitted hepatitis C virus (HCV) infections is extremely low in Australia. This study aims to conduct a cost‐effectiveness analysis of different testing strategies for HCV infection in blood donations.

Materials and Methods

The four testing strategies evaluated in this study were universal testing with both HCV antibody (anti‐HCV) and nucleic acid testing (NAT); anti‐HCV and NAT for first‐time donations and NAT only for repeat donations; anti‐HCV and NAT for transfusible component donations and NAT only for plasma for further manufacture; and universal testing with NAT only. A decision‐analytical model was developed to assess the cost‐effectiveness of alternative HCV testing strategies. Sensitivity analysis and threshold analysis were conducted to account for data uncertainty.

Results

The number of potential transfusion‐transmitted cases of acute hepatitis C and chronic hepatitis C was approximately zero in all four strategies. Universal testing with NAT only was the most cost‐effective strategy due to the lowest testing cost. The threshold analysis showed that for the current practice to be cost‐effective, the residual risks of other testing strategies would have to be at least 1 HCV infection in 2424 donations, which is over 60,000 times the baseline residual risk (1 in 151 million donations).

Conclusion

The screening strategy for HCV in blood donations currently implemented in Australia is not cost‐effective compared with targeted testing or universal testing with NAT only. Partial or total removal of anti‐HCV testing would bring significant cost savings without compromising blood recipient safety.
Prevalence of weak D phenotypes in the general population of Québec, Canada: A focus on weak D type 42
Authors: Drouin Mathieu , Rochette Samuel , St‐Louis Maryse , Lewin Antoine , Laganière Josée
Published: May 2023

Background and Objectives

Weak D type 42 accounts for an unusually high proportion of weak D phenotypes in Québec (Canada), which contrasts with other predominantly White populations. However, its prevalence in the general population is unknown. We estimated the prevalence of weak D type 42 and other common weak D phenotypes in Québec.

Materials and Methods

We screened forRHD*01W.42alleles among 1000 individuals of CARTaGENE—a cohort representative of Québec's population. The prevalence of weak D type 42 was calculated based on the allele frequency ofRHD*01W.42andd(i.e., all recessive alleles that confer a D− phenotype), assuming a Hardy–Weinberg equilibrium. This prevalence was then leveraged to calculate that of other common weak D phenotypes, using published prevalence estimates among weak D phenotypes.

Results

Two individuals harboured theRHD*01W.42/RHD*01heterozygous genotype. Assuming an allele frequency of 38.19% ford, the overall prevalence of weak D type 42 was 0.08%. The following prevalence estimates were also obtained: 0.44% for all weak D phenotypes and 0.07%, 0.01% and 0.04% for weak D types 1, 2 and 3, respectively.

Conclusion

Québec has the highest documented prevalence of weak D type 42, which was estimated at 0.08%.
Performance and Emission Characteristics for Karanja Biodiesel Blends Assisted With Green Hydrogen Fuel and Nanoparticles
Authors: Praveenkumar T. R. , Rath Badrinarayan , Devanesan Sandhanasamy , Alsahi Mohamad S. , Jhanani G. K. , Gemede Habtamu Fekadu , Solowski Gawel , Daniel Freedon
Published: May 2023

Extensive efforts are being made to produce and use a variety of alternative energies in order to meet the rising global energy demand. The main purpose of this research was to evaluate the mass fuel burnt, emissions, and performance properties of biodiesel made from non-edible karanja oil along with hydrogen and nanoparticles in a standard diesel engine. Upon experimental evaluation, it was determined that the transesterified karanja oil retained all of its vital physicochemical properties within the specified tolerances. The emission and performance characteristics of karanja biodiesel blended with nanoparticles and hydrogen fuel were assessed at different engine loads ranging from 0% to 100%. The uniform amount of nanoparticles and hydrogen was incorporated into test fuels. Al2O3 nanoparticles of 50 ppm were used in this study and hydrogen of 5 L/min was supplied to the engine. K20NH test fuel had the maximum brake thermal efficiency and less brake-specific fuel consumption compared to the other fuel blend. The emissions were considerably reduced on karanja oil, nanoparticles, and hydrogen-blended biodiesel except for nitrogen emission compared with conventional diesel fuel. In this study, the utilization of karanja, nanoparticles, and hydrogen-blended biodiesel showed a promising alternative to fossil fuels due to reduced emission and enhanced performance characteristics.
A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
Authors: Wang Yao , Qi Tao , Liu Jingtong , Yang Yuan , Wang Ziwen , Wang Ying , Wang Tianyi , Li Miaomiao , Li Mingqing , Lu Daru , Chang Alex Chia Yu , Yang Li , Gao Song , Wang Yongming , Lan Feng
Published: May 2023

The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide mutations cannot be discriminated from wild-type alleles by current CRISPR-Cas systems. Here, we functionally screened six Cas12j nucleases and determined Cas12j-8 as an ideal genome editor with a hypercompact size. Cas12j-8 displayed comparable activity to AsCas12a and Un1Cas12f1. Cas12j-8 is a highly specific nuclease sensitive to single-nucleotide mismatches in the protospacer adjacent motif (PAM)–proximal region. We experimentally proved that Cas12j-8 enabled allele-specific disruption of genes with a single-nucleotide polymorphism (SNP). Cas12j-8 recognizes a simple TTN PAM that provides for high target site density. In silico analysis reveals that Cas12j-8 enables allele-specific disruption of 25,931 clinically relevant variants in the ClinVar database, and 485,130,147 SNPs in the dbSNP database. Therefore, Cas12j-8 would be particularly suitable for therapeutic applications.
Flexible biomimetic block copolymer composite for temperature and long-wave infrared sensing
Authors: Kim Tae Hyun , Zhou Zhun , Choi Yeong Suk , Costanza Vincenzo , Wang Linghui , Bahng Joong Hwan , Higdon Nicholas J. , Yun Youngjun , Kang Hyunbum , Kim Sunghan , Daraio Chiara
Published: May 2023

Biological compounds often provide clues to advance material designs. Replicating their molecular structure and functional motifs in artificial materials offers a blueprint for unprecedented functionalities. Here, we report a flexible biomimetic thermal sensing (BTS) polymer that is designed to emulate the ion transport dynamics of a plant cell wall component, pectin. Using a simple yet versatile synthetic procedure, we engineer the physicochemical properties of the polymer by inserting elastic fragments in a block copolymer architecture, making it flexible and stretchable. The thermal response of our flexible polymer outperforms current state-of-the-art temperature sensing materials, including vanadium oxide, by up to two orders of magnitude. Thermal sensors fabricated from these composites exhibit a sensitivity that exceeds 10 mK and operate stably between 15° and 55°C, even under repeated mechanical deformations. We demonstrate the use of our flexible BTS polymer in two-dimensional arrays for spatiotemporal temperature mapping and broadband infrared photodetection.
Antimicrobial Agents and Chemotherapy
Launches a New Section Focused on Innovative Antimicrobial Stewardship Studies
Authors: Tamma Pranita D. , Arias Cesar A. , Shields Ryan K.
Published: May 2023

In response to the global burden of antimicrobial resistance (AMR) and the critical role antimicrobial stewardship plays in optimizing antibiotic use and reducing the subsequent emergence of AMR,
Antimicrobial Agents
and Chemotherapy
is excited to add a new section to the journal focused on antimicrobial stewardship studies. Combatting the devastating burden of AMR requires novel, multipronged approaches from clinicians and scientists alike.
Microbe-Mineral Interactions between Asbestos and Thermophilic Chemolithoautotrophic Anaerobes
Authors: Choi Jessica K. , Vigliaturo Ruggero , Gieré Reto , Pérez-Rodríguez Ileana
Published: May 2023

We explored the potential of chemosynthetic microorganisms growing at high temperatures to induce the release of key elements (mainly iron, silicon, and magnesium) involved in the known toxic properties (iron content and fibrous mineral shapes) of asbestos minerals. We show for the first time that the microbial respiration of iron from amphibole asbestos releases some of the iron contained in the mineral while supporting microbial growth.
Emergent Long-Lived Zitterbewegung in Su–Schrieffer–Heeger Lattice with Third-Nearest-Neighbor Hopping
Authors: Chen M.-N. , Yu X.-J. , Li Z.
Published: May 2023

We investigate the wavepacket dynamics of quasiparticles in a Su–Schrieffer–Heeger lattice with third-nearest-neighbor hopping. The results reveal that the life-span of Zitterbewegung can be prolonged. To better understand the mechanism, we discuss the band structure and the long-time average of inverse participation rate. The results show that the band structure can be effectively manipulated as a quasi-flat band by introducing the third-nearest-neighbor hopping. This, as a unique advantage over the standard Su–Schrieffer–Heeger model, will bring about restrained diffusion of the wavepacket as well as dramatically stretched life-span of Zitterbewegung, thus will promise wide applications in condensed matter physics.
Hypothesis on the Reasons for Strong Variability in the Concentrations of Impurities in Natural Waters
Authors: Danilov-Danilyan V. I. , Rozenthal O. M.
Published: May 2023

A hypothesis has been put forward about the formation of increased dispersion of the concentration of substances contaminating flowing river water under the influence of internal synergistic factors. The effect is manifested in the dispersion of monitored quality factors, exceeding their average values, which in practice makes it difficult to manage water use. It is assumed that such dispersion is a consequence of the nonlinearity of systems that are far from thermodynamic equilibrium. It is possible that the driving force in this case is the turbophoresis of impurity particles in a turbulent, as a rule, water flow.
Neotectonic Dislocations on the Barents Sea Shelf and Their Origin on the Basis of Morphometry of the Seafloor Relief, Seismic Survey, and the Deep Structure of the Mantle
Authors: Sokolov S. Yu. , Abramova A. S. , Shkarubo S. I.
Published: May 2023

A comprehensive analysis of the morphometric attribute “general curvature” for the Barents Sea bathymetry and reports on the seismic and seismoacoustic data on tectonic dislocations in a wave field, the fault network of the sedimentary cover, and seismotomographic data on the heterogeneous deep pattern of the distribution of seismic wave velocities in the upper mantle are provided. The analysis showed that mobile blocks of the upper mantle, exhibiting heterogeneous rheological properties, are associated with the consolidated part of the Earth’s crust fault network of deep-seated origin. The fault network emerges on the seafloor and, becoming a relief-forming factor, forms specific domains with different textures displayed in the morphometric attribute “general curvature.”
Navigating two ‘truths’: a qualitative study of physician-led end-of-life decision-making for children with life-limiting conditions
Authors: Vemuri Sidharth , Hynson Jenny , Williams Katrina , Gillam Lynn
Published: May 2023

Objective

To describe how paediatricians undertake the process of end-of-life decision-making for a child with a life-limiting condition who is unable to participate in decision-making for themselves.

Design

A qualitative phenomenological study using semistructured interviews based around a clinical vignette matched to the clinical practice of individual paediatricians. Verbatim transcripts underwent thematic analysis.

Setting

Paediatricians practising in Victoria (Australia) between mid-2019 and mid-2020.

Participants

Twenty-five purposively sampled paediatricians caring for children with specific life-limiting conditions: children with severe neurodisability, oncological or haematological malignancies or complex cardiac disease in an inpatient intensive care or outpatient clinic setting.

Results

A process of physician-led end-of-life decision-making was described. Paediatricians first contemplate that the child’s death is approaching, then prepare themselves by ensuring there are no reversible factors at play. They then inform parents of this view and, if needed, hold discordant views between parents and themselves about the child’s death in a ‘fruitful tension’. Ultimately, they seek to bring parents’ views of their child in line with theirs to facilitate goal alignment.

Conclusions

Paediatricians feel responsible for facilitating the alignment of parental understanding of the child’s health status with their own. This is achieved either through direction or by holding differences between parental and medical truths about the child’s health in tension to provide time, space, and clarity. This alignment was seen as key to enabling end-of-life treatment decisions, without which conflict in end-of-life decision-making can arise or persist.
Digital drilling-based determination of rock anisotropy and anisotropic effect on cutter wear
Authors: Wang Haoteng , He Mingming , Zhao Jianbin , Zhang Yonghao
Published: May 2023

Anisotropy affects the mechanical behaviours of rock, especially in the application of rock engineering. In this study, a digital drilling method is proposed to evaluate the mechanical anisotropy of rock and the anisotropic effect on cutter wear. In considering critical friction, the cutting efficiency and contact stress were determined from the revised drilling model in order to characterize the drilling process. For six types of rock, a series of drilling tests were conducted on three axial directions using a coring bit. The anisotropy of the rock strength was obtained from point load tests to compare with the anisotropy of the drilling characteristics. Correspondingly, an anisotropy criterion was established. A critical point was identified in the evolution of contact stress and in the plot of drilling parameters, corresponding to the critical friction. Results indicate that the evolution of contact stress with inclination angle suggests similar elastic and plastic stages (inclination angles of 5° and 12°, respectively). The typical evolution was also confirmed by the critical depth of the friction point. Moreover, the cutting efficiency and contact stress at the critical point showed the evident anisotropic characteristic. A comparison of two kinds of anisotropy indices,
A
1
and
A
2
, was conducted to determine the anisotropy index of the drilling characteristics. The contact stress gave the anisotropy sequence to be shale (22.45) > gneiss (14.21) > schist 302 (10.74) and blue sandstone (10.07) > granite (7.29) > red sandstone (5.09). An examination of consistency with strength anisotropy index suggested that the contact stress has a fitting correlation with an accuracy of 91%. In summary, the digital drilling-based method provides a reliable evaluation of rock anisotropy, showing potential for a practical application.
Accurate Open-Set Recognition for Memory Workload
Authors: Jang Jun-Gi , Shim Sooyeon , Egay Vladimir , Lee Jeeyong , Park Jongmin , Chae Suhyun , Kang U
Published: May 2023

How can we accurately identify new memory workloads while classifying known memory workloads? Verifying DRAM (Dynamic Random Access Memory) using various workloads is an important task to guarantee the quality of DRAM. A crucial component in the process is open-set recognition which aims to detect new workloads not seen in the training phase. Despite its importance, however, existing open-set recognition methods are unsatisfactory in terms of accuracy since they fail to exploit the characteristics of workload sequences.

In this article, we propose
Acorn
, an accurate open-set recognition method capturing the characteristics of workload sequences.
Acorn
extracts two types of feature vectors to capture sequential patterns and spatial locality patterns in memory access.
Acorn
then uses the feature vectors to accurately classify a subsequence into one of the known classes or identify it as the unknown class. Experiments show that
Acorn
achieves state-of-the-art accuracy, giving up to 37% points higher unknown class detection accuracy while achieving comparable known class classification accuracy than existing methods.
Continuous Improvement of the Rational Use of Central Nervous System Disease-Related Drugs in Elderly Inpatients
Authors: Li Fang , Zhu Yingli , Li Min , Xu Yinpeng , Cui Yahui , Yang Ying , Wang Yaling , Hao Hong
Published: May 2023

What Is Known? In elderly inpatients, potential inappropriate medication (PIM) is a prominent prescription challenge. However, there is limited information available regarding PIM in patients with central nervous system (CNS) diseases in China. Objective. To evaluate and improve the rational use of drugs for the treatment of CNS diseases in elderly inpatients. Method. A retrospective, cross-sectional study was conducted among elderly inpatients (≥65 years) admitted to the Ninth People’s Hospital of Zhengzhou in China from March 2020 to March 2021. PIM was identified based on the 2019 Beers criteria at admission and discharge. The patients recruited in March 2020 were considered a baseline group, which was used as a comparison to evaluate PIM of CNS disease-related drugs in June 2020, September 2020, December 2020, and March 2021. Results. A total of 1500 patients were included in the evaluation. There was a statistically significant difference in the number of average hospitalization days, drug varieties used, and PIM detection (

p
<
0.05

), as determined by X2 test. A total of 332 cases of PIM were identified, and 226 cases were detected for the interaction with CNS disease dementia. Multifactor logistic regression analysis showed that male, length of stay ≥15 days, and >10 medication types were risk factors for the occurrence of PIM (

p
<
0.05

). After clinical supervision and training based on the High-Risk Drug Replacement Program for the Elderly, the rate of irrational medication under medical advice decreased from 34.67% in March 2020 to 14.0% (

p
<
0.001

) in March 2021. What Is New and Conclusion. There was certain rationality based on the High-Risk Drug Replacement Program for the Elderly, and the rates of selective serotonin reuptake inhibitor, 5-hydroxylamine/norepinephrine re-intake inhibitor, rotenone, quetiapine, and proton pump inhibitor use were improved. These results provide a reference for the continuous improvement of the PIM catalog for elderly patients.
Local Corner and Motion Key Point Trajectory Extraction for Facial Forgery Identification
Authors: Liu Qingtong , Xue Ziyu
Published: May 2023

At present, the development of deep forgery technology has brought new challenges to media content forensics, and the use of deep forgery identification methods to identify forged audio and video has become a significant focus of research and difficulty. Deep forgery technology and forensic technology play a mutual game and promote each other’s development. This paper proposes a spatiotemporal local feature abstraction (STLFA) framework for facial forgery identification to solve the media industry challenges of deep forgery technology. To adequately utilize local facial features, we combine facial key points, key point movement, and facial corner points to detect forgery content. This paper establishes a spatiotemporal relation, which realizes face forgery detection by identifying abnormalities of facial keypoints and corner points for interframe judgments. Meanwhile, we utilize RNNs to predict the sequences from facial key point movement abnormalities and corner points for interframe. Experimental results show that our method achieves better performance than some existing methods and good anticompression forgery face detection performance on FF++.
Molecular Evolution of Interferon-Epsilon (IFNε) Pseudogene Modulates Innate and Specific Antiviral Immunity in Manis javanica
Authors: Ahmad Hafiz Ishfaq , Jameel Laraib , Zahra Quratulain , Zhou Jiabin , Li Linmiao , Zhang Xiujuan , Ahmed Shakeel , Ali Daoud , Kumar Gokhlesh , Safdar Aleena , Abbas Farhan , Chen Jinping
Published: May 2023

Interferon-Epsilon (IFNε) is a type of interferon, a protein that plays a role in the immune response to viral infections. This study is aimed at examining the molecular evolution of the IFNε pseudogene in Manis javanica, and it has been found to modulate the innate and specific antiviral immunity in this species. In this study, we identified that IFNε gene has undergone rapid evolution in Manis javanica, with the human and primate IFNε genes showing evidence of positive selection. This suggests that IFNε has played an important role in the evolution of the immune system, possibly in response to coevolution with viral pathogens. Comparative genomic analysis revealed that the IFNε pseudogene in pangolins originated from a gene duplication event approximately 48 million years ago. It subsequently lost its protein-coding function due to multiple deleterious mutations. However, the IFNε pseudogene exhibits a high degree of conservation in its promoter region, suggesting it may still play a regulatory role in antiviral immunity. This suggests that the pseudogene may have evolved to serve an important function in the pangolin’s immune system, potentially helping to protect it from viral infections. The molecular evolution of IFNε provides insights into the coevolutionary dynamics between host immune systems and viral pathogens and may have implications for developing new antiviral therapies.
Point of Common Coupling Voltage Modulated Direct Power Control of Grid-Tied Photovoltaic Inverter for AC Microgrid Application
Authors: Ahmad Shameem , Mubarak Hamza , Jhuma Umme Kulsum , Ahmed Tofael , Mekhilef Saad , Mokhlis Hazlie
Published: May 2023

A direct power control (DPC) approach is proposed in this study for a grid-tied photovoltaic (PV) voltage source inverter (VSI) to regulate active and reactive power flow directly in between utility grid and microgrid (MG) by controlling point of common coupling (PCC) voltage. The proposed PCC voltage modulated (PVM) theory-based DPC method (PVMT-DPC) is composed of nonlinear PVM, nonlinear damping, conventional feedforward, and feedback PI controllers. For grid synchronization rather than employing phase-locked-loop (PLL) technology, in this study, direct power calculation of the PCC voltage and current is adopted. Subsequently, at PCC, the computed real and reactive powers are compared with reference powers in order to generate the VSI’s control signals using sinusoidal pulse width modulation (SPWM). Because of the absence of the PLL and DPC method adoption, the suggested controller has a faster convergence rate compared to traditional VSI’s power controllers. Additionally, it displays nearly zero steady-state power oscillations, which assure that MG’s power quality is improved significantly. To validate the proposed PVMT-DPC method’s performance, real-time simulations are conducted via real-time digital simulator (RTDS) for a variety of cases. The obtained results demonstrate that using the proposed PVMT-DPC approach, PV VSI can track the reference power within 0.055 s where the output power has low steady-state oscillations and output current has lower total harmonic distortion (THD) of 1.68%.
FACSC: Fine-Grained Access Control Based on Smart Contract for Terminals in Software-Defined Network
Authors: Jiang Bingcheng , He Qian , He Mingliu , Zhai Zhongyi , Zhao Baokang
Published: May 2023

Physical terminals provide network services to upper-layer applications, but their limited memory and processing power make it challenging to perform security updates and patches, leaving them vulnerable to known security threats. Attackers can exploit these weaknesses to control the terminals and attack the network. To restrict unauthorized access to the network and its resources, appropriate access control mechanisms are necessary. In this paper, we propose a fine-grained access control method based on smart contracts (FACSC) for terminals in software-defined networking (SDN). FACSC utilizes the attribute-based access control (ABAC) model to achieve fine-grained control over terminal access networks. To ensure the security and reliability of access control policies and terminal-related attribute information, we utilize smart contract technology to implement the ABAC model. Furthermore, we leverage the programming protocol-independent packet processor (P4) to filter and forward packets in the data plane based on the packet option field, enabling rapid terminal access. Experimental results show that our proposed method achieves fine-grained secure authentication of terminals in SDN networks with a low authentication processing overhead.
Table S3 from ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells
Authors: Hu Qiangsheng , Dai Jie , Zhang Zheng , Yu Huansha , Zhang Jing , Zhu Xinsheng , Qin Yi , Zhang Lele , Zhang Peng
Published: May 2023

Supplementary RNA sequencing raw data
Table S1 from ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells
Authors: Hu Qiangsheng , Dai Jie , Zhang Zheng , Yu Huansha , Zhang Jing , Zhu Xinsheng , Qin Yi , Zhang Lele , Zhang Peng
Published: May 2023

Supplementary Table 1 Primer sequences
Supplementary Figure 3 from Hypoxia Potentiates the Inflammatory Fibroblast Phenotype Promoted by Pancreatic Cancer Cell–Derived Cytokines
Authors: Schwörer Simon , Cimino Francesco V. , Ros Manon , Tsanov Kaloyan M. , Ng Charles , Lowe Scott W. , Carmona-Fontaine Carlos , Thompson Craig B.
Published: May 2023

Supplementary Figure 3, related to Figure 3
Supplementary Figure 1 from Hypoxia Potentiates the Inflammatory Fibroblast Phenotype Promoted by Pancreatic Cancer Cell–Derived Cytokines
Authors: Schwörer Simon , Cimino Francesco V. , Ros Manon , Tsanov Kaloyan M. , Ng Charles , Lowe Scott W. , Carmona-Fontaine Carlos , Thompson Craig B.
Published: May 2023

Supplementary Figure 1, related to Figure 1
Supplementary Methods from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

Supplementary Methods
Supplementary Tables from NAT10 Drives Cisplatin Chemoresistance by Enhancing ac4C-Associated DNA Repair in Bladder Cancer
Authors: Xie Ruihui , Cheng Liang , Huang Ming , Huang Lin , Chen Ziyue , Zhang Qiang , Li Hong , Lu Junlin , Wang Hongjin , Zhou Qianghua , Huang Jian , Chen Xu , Lin Tianxin
Published: May 2023

Supplementary Tables
Figure S6 from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

MLH1 KO cells do not display MSI.
Figure S3 from BRCA2 Germline Mutations Identify Gastric Cancers Responsive to PARP Inhibitors
Authors: Petrelli Annalisa , Rizzolio Sabrina , Pietrantonio Filippo , Bellomo Sara E. , Benelli Matteo , De Cecco Loris , Romagnoli Dario , Berrino Enrico , Orrù Claudia , Ribisi Salvatore , Moya-Rull Daniel , Migliore Cristina , Conticelli Daniela , Maina Irene M. , Puliga Elisabetta , Serra Violeta , Pellegrino Benedetta , Llop-Guevara Alba , Musolino Antonino , Siena Salvatore , Sartore-Bianchi Andrea , Prisciandaro Michele , Morano Federica , Antista Maria , Fumagalli Uberto , De Manzoni Giovanni , Degiuli Maurizio , Baiocchi Gian Luca , Amisano Marco F. , Ferrero Alessandro , Marchiò Caterina , Corso Simona , Giordano Silvia
Published: May 2023

Familial history of cancers of the GEA patients carrying BRCA2 germline LOF mutations.
Supplementary Table S2 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Significant genes in 10 clusters identified through spatial transcriptomics of brain tissues from a GBM mouse model.
Supplementary Table S1 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Characterization of patients whose resected tissues were used for confocal microscopy.
Supplementary Fig. S9 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Additional analysis LRP6 and the Wnt/β-catenin pathway activation in BTICs.
Supplementary Fig. S7 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Perivascular location assessments of biglycan expression in the human GBM microenvironment.
Supplementary Fig. S5 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Spatial expression of stemness genes in tumor areas.
Supplementary Fig. S1 from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Quality control analysis of spatial transcriptomics.
Graphical abstract from Single-Cell Spatial Analysis Identifies Regulators of Brain Tumor–Initiating Cells
Authors: Mirzaei Reza , D'Mello Charlotte , Liu Marina , Nikolic Ana , Kumar Mehul , Visser Frank , Bose Pinaki , Gallo Marco , Yong V. Wee
Published: May 2023

Graphical abstract. A multi-omics analysis of the glioblastoma tumor microenvironment revealed that biglycan, predominantly expressed by inflammatory BTICs, drives tumor growth. The graphics were generated with Biorender.com.
Supplementary Figure S6 from Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft Model
Authors: Chen Shih-Shih , Barrientos Jacqueline C. , Ferrer Gerardo , King-Richards Morgan , Chen Yu-Ju , Ravichandran Priyadarshini , Ibrahim Michael , Kieso Yasmine , Waters Sheila , Kutok Jeffery L. , Peluso Marisa , Sharma Sujata , Weaver David T. , Pachter Jonathan A. , Rai Kanti R. , Chiorazzi Nicholas
Published: May 2023

Effects of treatment with duvelisib and ibrutinib on the numbers of T cells in in mice receiving samples from the same patients in Figure 6.
Figure S6 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: May 2023

Figure S6: Number of H2AX-foci after the treatment of saline and [225Ac]DOTA-YS5 for 7 days and 14 days (n=3). t-test p value is indicated as * p<0.05.
Figure S5 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: May 2023

Figure S5: Estimated equivalent doses (in Sv) in organs and tumor indicating the highest dose (37 Sv) was delivered to tumor tissue.
Figure S3 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: May 2023

Figure S3: Binding of [225Ac]DOTA-YS5 to 22Rv1 with or without cold antibody blocking. Blocking with cold YS5 results in a reduction of binding from 19.25±0.70% to 4.46±0.17% (n=3).
Supplementary Figure S1 from Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft Model
Authors: Chen Shih-Shih , Barrientos Jacqueline C. , Ferrer Gerardo , King-Richards Morgan , Chen Yu-Ju , Ravichandran Priyadarshini , Ibrahim Michael , Kieso Yasmine , Waters Sheila , Kutok Jeffery L. , Peluso Marisa , Sharma Sujata , Weaver David T. , Pachter Jonathan A. , Rai Kanti R. , Chiorazzi Nicholas
Published: May 2023

Effects of inhibition of PI3K isoforms on CLL-cell proliferation, survival, and migration.
Table S8 from Immunologic Predictors for Clinical Responses during Immune Checkpoint Blockade in Patients with Myelodysplastic Syndromes
Authors: Lee Sung-Eun , Wang Feng , Grefe Maison , Trujillo-Ocampo Abel , Ruiz-Vasquez Wilfredo , Takahashi Koichi , Abbas Hussein A. , Borges Pamella , Antunes Dinler Amaral , Al-Atrash Gheath , Daver Naval , Molldrem Jeffrey J. , Futreal Andrew , Garcia-Manero Guillermo , Im Jin S.
Published: May 2023

Table S8. Genes used for gene set analysis
Figure S1 from Treatment of Prostate Cancer with CD46-targeted 225Ac Alpha Particle Radioimmunotherapy
Authors: Bidkar Anil P. , Wang Sinan , Bobba Kondapa Naidu , Chan Emily , Bidlingmaier Scott , Egusa Emily A. , Peter Robin , Ali Umama , Meher Niranjan , Wadhwa Anju , Dhrona Suchi , Dasari Chandrashekhar , Beckford-Vera Denis , Su Yang , Tang Ryan , Zhang Li , He Jiang , Wilson David M. , Aggarwal Rahul , VanBrocklin Henry F. , Seo Youngho , Chou Jonathan , Liu Bin , Flavell Robert R.
Published: May 2023

Figure S1: Immunohistochemical staining for PSMA expression on tissue section from patient-derived xenografts. Scale bar = 200 µm
Supplementary PLS from Randomized Trial of Tepotinib Plus Gefitinib versus Chemotherapy in EGFR-Mutant NSCLC with EGFR Inhibitor Resistance Due to MET Amplification: INSIGHT Final Analysis
Authors: Liam Chong Kin , Ahmad Azura Rozila , Hsia Te-Chun , Zhou Jianying , Kim Dong-Wan , Soo Ross Andrew , Cheng Ying , Lu Shun , Shin Sang Won , Yang James Chih-Hsin , Zhang Yiping , Zhao Jun , Berghoff Karin , Bruns Rolf , Johne Andreas , Wu Yi-Long
Published: May 2023

Plain language summary
Supplementary Figure S4 from Randomized Trial of Tepotinib Plus Gefitinib versus Chemotherapy in EGFR-Mutant NSCLC with EGFR Inhibitor Resistance Due to MET Amplification: INSIGHT Final Analysis
Authors: Liam Chong Kin , Ahmad Azura Rozila , Hsia Te-Chun , Zhou Jianying , Kim Dong-Wan , Soo Ross Andrew , Cheng Ying , Lu Shun , Shin Sang Won , Yang James Chih-Hsin , Zhang Yiping , Zhao Jun , Berghoff Karin , Bruns Rolf , Johne Andreas , Wu Yi-Long
Published: May 2023

Treatment-related adverse events in treated patients with MET amplification and/or MET IHC 2+/3+
Table S3 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: May 2023

A. Overall Survival outcomes and B. Progression Free Survival outcomes utilizing multivariate analysis for PSMA positive CTCs, adjusting on prior treatment, risk score, CTC enumeration, comparting 3 CTC + groups with the reference group being CTC 0.