bluuebunny/crossref_metadata_2025 huggingface dataset:
Bioaugmentation Technology for Treatment of Toxic and Refractory Organic Waste Water Based on Artificial Intelligence
Authors: Yanbo Jiang , Jianyi Jiang , Xiandong Wei , Wei Ling , Lincheng Jiang
Published: July 2021

With the development of modern chemical synthesis technology, toxic and harmful compounds increase sharply. In order to improve the removal efficiency of refractory organic matter in waste water, the method of adding powdered activated carbon (PAC) to the system for adsorption was adopted. Through the analysis of organic matter removal rule before and after waste water treatment, it can be found that PAC is easy to adsorb hydrophobic organic matter, while activated sludge is easy to remove hydrophilic and weakly hydrophobic neutral organic matter. Powdered activated carbon-activated sludge SBR system (PAC-AS) system is obviously superior to AS and PAC system in removing organic matter of hydrophilic and hydrophobic components, that is, biodegradation and PAC adsorption are additive. Compared with the control system, the Chemical Oxygen Demand (COD) removal rate of refractory substances increased by 8.36%, and PAC had a good adsorption effect on small molecular weight organic compounds, but with the increase of molecular weight of organic compounds, the adsorption effect of PAC gradually weakened, and it had no adsorption effect on macromolecular organic compounds. Based on the research of fuzzy control theory, an Agent control system for ozone oxidation process of industrial waste water based on Mobile Agent Server (MAS) theory was established, which was realized by fuzzy control method. The simulation results showed strong stability and verified the feasibility and adaptability of the distributed intelligent waste water treatment system based on MAS theory in the actual control process.
Trehalose-Induced Remodelling of the Human Microbiota Affects Clostridioides difficile Infection Outcome in an In Vitro Colonic Model: A Pilot Study
Authors: Buckley Anthony M. , Moura Ines B. , Arai Norie , Spittal William , Clark Emma , Nishida Yoshihiro , Harris Hannah C. , Bentley Karen , Davis Georgina , Wang Dapeng , Mitra Suparna , Higashiyama Takanobu , Wilcox Mark H.
Published: July 2021

Within the human intestinal tract, dietary, microbial- and host-derived compounds are used as signals by many pathogenic organisms, including Clostridioides difficile. Trehalose has been reported to enhance virulence of certain C. difficile ribotypes; however, such variants are widespread and not correlated with clinical outcomes for patients suffering from C. difficile infection (CDI). Here, we make preliminary observations on how trehalose supplementation affects the microbiota in an in vitro model and show that trehalose-induced changes can reduce the outgrowth of C. difficile, preventing simulated CDI. Three clinically reflective human gut models simulated the effects of sugar (trehalose or glucose) or saline ingestion on the microbiota. Models were instilled with sugar or saline and further exposed to C. difficile spores. The recovery of the microbiota following antibiotic treatment and CDI induction was monitored in each model. The human microbiota remodelled to utilise the bioavailable trehalose. Clindamycin induction caused simulated CDI in models supplemented with either glucose or saline; however, trehalose supplementation did not result in CDI, although limited spore germination did occur. The absence of CDI in trehalose model was associated with enhanced abundances of Finegoldia, Faecalibacterium and Oscillospira, and reduced abundances of Klebsiella and Clostridium spp., compared with the other models. Functional analysis of the microbiota in the trehalose model revealed differences in the metabolic pathways, such as amino acid metabolism, which could be attributed to prevention of CDI. Our data show that trehalose supplementation remodelled the microbiota, which prevented simulated CDI, potentially due to enhanced recovery of nutritionally competitive microbiota against C. difficile.
Seasonal Sleep Variations and Their Association With Meteorological Factors: A Japanese Population Study Using Large-Scale Body Acceleration Data
Authors: Li Li , Nakamura Toru , Hayano Junichiro , Yamamoto Yoshiharu
Published: July 2021

Seasonal changes in meteorological factors [e.g., ambient temperature (Ta), humidity, and sunlight] could significantly influence a person's sleep, possibly resulting in the seasonality of sleep properties (timing and quality). However, population-based studies on sleep seasonality or its association with meteorological factors remain limited, especially those using objective sleep data. Japan has clear seasonality with distinctive changes in meteorological variables among seasons, thereby suitable for examining sleep seasonality and the effects of meteorological factors. This study aimed to investigate seasonal variations in sleep properties in a Japanese population (68,604 individuals) and further identify meteorological factors contributing to sleep seasonality. Here we used large-scale objective sleep data estimated from body accelerations by machine learning. Sleep parameters such as total sleep time, sleep latency, sleep efficiency, and wake time after sleep onset demonstrated significant seasonal variations, showing that sleep quality in summer was worse than that in other seasons. While bedtime did not show clear seasonality, get-up time varied seasonally, with a nadir during summer, and positively correlated with the sunrise time. Estimated by the abovementioned sleep parameters, Ta had a practically meaningful association with sleep quality, indicating that sleep quality worsened with the increase of Ta. This association would partly explain seasonal variations in sleep quality among seasons. In conclusion, Ta had a principal role for seasonality in sleep quality, and the sunrise time chiefly determined the get-up time.
An Anecdotal Case Study in Psychological Anthropology of Two Retired Middle School Teachers’ Perceptions of Classroom Life in the United States
Authors: Attwood Adam I.
Published: July 2021

In this qualitative study, interviews of two former middle school teachers were conducted and analyzed for how they fostered a supportive classroom environment in the United States despite the national stress of the Vietnam War during the late 1960s and early 1970s. Half a century later in 2020-2021, similar trends seem to remain just as important in society though the context has shifted. Implications for social-emotional learning from then and now are discussed. Each of the two participants in this study established centralized control through slightly different means and both used written guidelines and rules while emphasizing prosocial behaviors. Three themes emerged: 1) a syllabus was important in management style, 2) sports, and 3) the national social context—such as the Vietnam War—manifested itself in the discourse of the social environment to affect classroom life. Implications for the socio-spatial context of school architectural layout are explored.
Throw Them All in One Pot? Differences in Stereotypes About Subgroups of Pre-Service Teachers
Authors: Stuckert Martin O. , Ihme Toni A. , Südkamp Anna , Möller Jens
Published: July 2021

According to the stereotype content model, stereotypes can be described by using the dimensions competence and warmth. Compared to other professions, teaching is associated with a paternalistic stereotype consisting of high warmth and low competence. In four studies, stereotypes about different subgroups of pre-service teachers were compared. The aim was to understand sub-stereotypes better that could lead to different levels of stereotype threat and adverse behavioral tendencies. In Study 1 (N = 335), we compared stereotypes about elementary school pre-service teachers, grammar school pre-service teachers, computer science students, law students, and psychology students reported by pre-service teachers and psychology students. In contrast to nonteaching students, both groups of pre-service teachers corresponded to the paternalistic stereotype. In Study 2 (N = 243), pre-service teachers reported stereotypes about pre-service teachers for elementary schools, special education schools, comprehensive schools, vocational schools, and grammar schools. Elementary school pre-service teachers were stereotyped most paternalistically, while grammar school pre-service teachers matched the paternalistic stereotype the least. The ratings of other school types mostly fell between these extremes. In Studies 3a (N = 133, open-ended questions) and 3b (N = 308, closed-ended questions), students of various study programs compared pre-service teachers majoring in German and history (representing a non-STEM major combination) to pre-service teachers with the majors mathematics and physics (representing a STEM major combination). Pre-service teachers studying German and history were rated warmer but less competent than pre-service teachers with the majors mathematics and physics, confirmed by both methods of measuring stereotypes. In Studies 1, 3a, and 3b, ingroup favoritism in the ratings by pre-service teacher participants was tested and only found for competence in Study 1. The importance of our results and their implications for stereotype threat effects and possible interventions are discussed.
Whole-Exome Sequencing Identified CFTR Variants in Two Consanguineous Families in China
Authors: Yang Binyi , Lei Cheng , Yang Danhui , Tan Zhiping , Guo Ting , Luo Hong
Published: July 2021

Background

Cystic fibrosis (CF) is an autosomal recessive disease caused by genetic variants of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. It is a common hereditary disease in Caucasians while rare in the Chinese. Until now, only 87 Chinese patients have been reported with molecular confirmations. The variant spectrum and clinical features of Chinese CF patients are obviously different from those of Caucasians.

Materials and Methods

Whole-exome sequencing was applied to analyze the exome of three individuals who have only the typical CF phenotype in the respiratory system from two consanguineous families. The protein domain and structure analysis were applied to predict the impact of the variants. Sanger sequencing was applied to validate the candidate variants.

Results

A previously reported homozygous variant in CFTR (NM_000492.4: c.1000C > T, p.R334W) was identified in proband I. A novel homozygous variant in a polymorphic position (NM_000492.4: c.1409T > A, p.V470E) was identified in two individuals in the family II. The novel CFTR variant predicted to be disease-causing is the first, to the best of our knowledge, to be reported in CFTR. However, in vitro validation is still needed.

Conclusion

Our finding expands the variant spectrum of CFTR, reveals clearer clinical phenotype distinction and variant spectrum distinction between Chinese and Caucasian CF patients, and contributes to a more rapid genetic diagnosis and future genetic counseling.
Impact of Genetic Variation in Gene Regulatory Sequences: A Population Genomics Perspective
Authors: Joshi Manas , Kapopoulou Adamandia , Laurent Stefan
Published: July 2021

The unprecedented rise of high-throughput sequencing and assay technologies has provided a detailed insight into the non-coding sequences and their potential role as gene expression regulators. These regulatory non-coding sequences are also referred to as cis-regulatory elements (CREs). Genetic variants occurring within CREs have been shown to be associated with altered gene expression and phenotypic changes. Such variants are known to occur spontaneously and ultimately get fixed, due to selection and genetic drift, in natural populations and, in some cases, pave the way for speciation. Hence, the study of genetic variation at CREs has improved our overall understanding of the processes of local adaptation and evolution. Recent advances in high-throughput sequencing and better annotations of CREs have enabled the evaluation of the impact of such variation on gene expression, phenotypic alteration and fitness. Here, we review recent research on the evolution of CREs and concentrate on studies that have investigated genetic variation occurring in these regulatory sequences within the context of population genetics.
ImputEHR: A Visualization Tool of Imputation for the Prediction of Biomedical Data
Authors: Zhou Yi-Hui , Saghapour Ehsan
Published: July 2021

Electronic health records (EHRs) have been widely adopted in recent years, but often include a high proportion of missing data, which can create difficulties in implementing machine learning and other tools of personalized medicine. Completed datasets are preferred for a number of analysis methods, and successful imputation of missing EHR data can improve interpretation and increase our power to predict health outcomes. However, use of the most popular imputation methods mainly require scripting skills, and are implemented using various packages and syntax. Thus, the implementation of a full suite of methods is generally out of reach to all except experienced data scientists. Moreover, imputation is often considered as a separate exercise from exploratory data analysis, but should be considered as art of the data exploration process. We have created a new graphical tool, ImputEHR, that is based on a Python base and allows implementation of a range of simple and sophisticated (e.g., gradient-boosted tree-based and neural network) data imputation approaches. In addition to imputation, the tool enables data exploration for informed decision-making, as well as implementing machine learning prediction tools for response data selected by the user. Although the approach works for any missing data problem, the tool is primarily motivated by problems encountered for EHR and other biomedical data. We illustrate the tool using multiple real datasets, providing performance measures of imputation and downstream predictive analysis.
Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer
Authors: Wang Zhenxing , Wang Xi , Wang Yaobang , Tang Shaomei , Feng Chao , Pan Lixin , Lu Qinchen , Tao Yuting , Xie Yuanliang , Wang Qiuyan , Tang Zhong
Published: July 2021

Small nuclear RNA is a class of non-coding RNA that widely exist in the nucleus of eukaryotes. Accumulated evidences have shown that small nuclear RNAs are associated with the regulation of gene expression in various tumor types. To explore the gene expression changes and its potential effects mediated by U11 snRNA in bladder cancer cells, U11 snRNA knockout and overexpressed cell lines were constructed and further used to analyze the gene expression changes by RNA sequencing. The differentially expressed genes were found to be mainly enriched in tumor-related pathways both in the U11 knockout and overexpression cell lines, such as NF-kappa B signaling pathway, bladder cancer and PI3K-Akt signaling pathway. Furthermore, alternative splicing events were proposed to participate in the potential regulatory mechanism induced by the U11 knockout or overexpression. In conclusion, U11 may be involved in the regulation of gene expression in bladder cancer cells, which may provide a potentially new biomarker for clinical diagnosis and treatment of bladder cancer.
Clinicopathologic Features and Risk Factors of Proteinuria in Transplant Glomerulopathy
Authors: Zhang Qiang , Budde Klemens , Schmidt Danilo , Halleck Fabian , Duerr Michael , Naik Marcel G. , Mayrdorfer Manuel , Duettmann Wiebke , Klauschen Frederick , Rudolph Birgit , Wu Kaiyin
Published: July 2021

Background: Transplant glomerulopathy (TG) is one of the main causes of post-transplant proteinuria (PU). The features and possible risk factors for proteinuria in TG patients are uncertain.

Methods: We investigated all patients who had biopsy-proven TG from 2000 to 2018 in our center. The clinical and histological data were compared between two groups with or without PU (cut-off = 0.3 g/day). Spearman correlation analysis was used to evaluate the relationship between PU and pathological changes. The risk factors for PU in TG patients were determined by multivariable logistic regression analysis.

Results: One hundred and twenty-five (75.76%) of all enrolled 165 TG patients had proteinuria ≥0.3 g/day at the time of biopsy. TG patients' PU level was significantly correlated with Banff lesion score cg (ρ = 0.247, P = 0.003), and mm (ρ = 0.257, P = 0.012). Systolic blood pressure ≥140 mmHg (OR 2.72, 95% CI 1.04–7.10, P = 0.041), diastolic blood pressure ≥90 mmHg (OR 4.84, 95% CI 1.39–16.82, P = 0.013), peak PRA ≥5% (OR 6.47, 95% CI 1.67–25.01, P = 0.007), positive C4d staining (OR 4.55, 95% CI 1.29–16.11, 0.019), tacrolimus-based regimen (OR 3.5, 95% CI 1.28–9.54, P = 0.014), and calcium channel blocker usage (OR 4.38, 95% CI 1.59–12.09, P = 0.004) were independent risk factors for PU.

Conclusions: Proteinuria is common in TG patients. systolic blood pressure ≥140 mmHg, diastolic blood pressure ≥90 mmHg, peak PRA ≥5%, positive C4d staining, tacrolimus-based regimen, and calcium channel blocker usage are associated with proteinuria in TG patients.
Acquisition of Tigecycline Resistance by Carbapenem-Resistant Klebsiella pneumoniae Confers Collateral Hypersensitivity to Aminoglycosides
Authors: Chen Hua-le , Jiang Yan , Li Mei-mei , Sun Yao , Cao Jian-ming , Zhou Cui , Zhang Xiao-xiao , Qu Yue , Zhou Tie-li
Published: July 2021

Tigecycline is a last-resort antibiotic for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP). This study aimed to broaden our understanding of the acquisition of collateral hypersensitivity by CRKP, as an evolutionary trade-off of developing resistance to tigecycline. Experimental induction of tigecycline resistance was conducted with tigecycline-sensitive CRKP clinical isolates. Antimicrobial susceptibility testing, microbial fitness assessment, genotypic analysis and full-genome sequencing were carried out for these clinical isolates and their resistance-induced descendants. We found that tigecycline resistance was successfully induced after exposing CRKP clinical isolates to tigecycline at gradually increased concentrations, at a minor fitness cost of bacterial cells. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) found higher expression of the efflux pump gene acrB (5.3–64.5-fold) and its regulatory gene ramA (7.4–65.8-fold) in resistance-induced strains compared to that in the tigecycline-sensitive clinical isolates. Stable hypersensitivities to aminoglycosides and other antibiotics were noticed in resistance-induced strains, showing significantly lowered MICs (X 4 – >500 times). Full genome sequencing and plasmid analysis suggested the induced collateral hypersensitivity might be multifaceted, with the loss of an antimicrobial resistance (AMR) plasmid being a possible major player. This study rationalized the sequential combination of tigecycline with aminoglycosides for the treatment of CRKP infections.
Insights Into the Phylogenetic Distribution, Diversity, Structural Attributes, and Substrate Specificity of Putative Cyanobacterial Orthocaspases
Authors: Bhattacharjee Samujjal , Kharwar Surbhi , Mishra Arun Kumar
Published: July 2021

The functionality of caspase homologs in prokaryotic cell execution has been perceived, yet the dimensions of their metabolic pertinence are still cryptic. Here, a detailed in silico study on putative cyanobacterial caspase homologs, termed orthocaspases, in a sequenced genome of 132 strains was performed. We observed that 473 putative orthocaspases were distributed among 62% cyanobacterial strains subsumed within all the taxonomical orders. However, high diversity among these orthocaspases was also evident as the conventional histidine–cysteine (HC) dyad was present only in 72.03% of orthocaspases (wild-type), whereas the rest 28.18% were pseudo-variants having substituted the catalytic dyad. Besides, the presence of various accessory functional domains with Peptidase C14 probably suggested the multifunctionality of the orthocaspases. Moreover, the early origin and emergence of wild-type orthocaspases were conferred by their presence in Gloeobacter; however, the complex phylogeny displayed by these caspase-homologs perhaps suggested horizontal a gene transfer for their acquisition. However, morpho-physiological advancements and larger genome size favored the acquisition of orthocaspases. Moreover, the conserved caspase hemoglobinase fold not only in the wild-type but also in the pseudo-orthocaspases in Nostoc sp. PCC 7120 ascertained the least effect of catalytic motifs in the protein tertiary structure. Further, the 100-ns molecular dynamic simulation and molecular mechanics/generalized born surface area exhibited stable binding of arginylarginine dipeptide with wild-type orthocaspase of Nostoc sp. PCC 7120, displaying arginine-P1 specificity of wild-type orthocaspases. This study deciphered the distribution, diversity, domain architecture, structure, and basic substrate specificity of putative cyanobacterial orthocaspases, which may aid in functional investigations in the future.
RNA 3D Structure Prediction Using Coarse-Grained Models
Authors: Li Jun , Chen Shi-Jie
Published: July 2021

The three-dimensional (3D) structures of Ribonucleic acid (RNA) molecules are essential to understanding their various and important biological functions. However, experimental determination of the atomic structures is laborious and technically difficult. The large gap between the number of sequences and the experimentally determined structures enables the thriving development of computational approaches to modeling RNAs. However, computational methods based on all-atom simulations are intractable for large RNA systems, which demand long time simulations. Facing such a challenge, many coarse-grained (CG) models have been developed. Here, we provide a review of CG models for modeling RNA 3D structures, compare the performance of the different models, and offer insights into potential future developments.
Presurgical Evaluation of Epilepsy Using Resting-State MEG Functional Connectivity
Authors: Xu Na , Shan Wei , Qi Jing , Wu Jianping , Wang Qun
Published: July 2021

Epilepsy is caused by abnormal electrical discharges (clinically identified by electrophysiological recording) in a specific part of the brain [originating in only one part of the brain, namely, the epileptogenic zone (EZ)]. Epilepsy is now defined as an archetypical hyperexcited neural network disorder. It can be investigated through the network analysis of interictal discharges, ictal discharges, and resting-state functional connectivity. Currently, there is an increasing interest in embedding resting-state connectivity analysis into the preoperative evaluation of epilepsy. Among the various neuroimaging technologies employed to achieve brain functional networks, magnetoencephalography (MEG) with the excellent temporal resolution is an ideal tool for estimating the resting-state connectivity between brain regions, which can reveal network abnormalities in epilepsy. What value does MEG resting-state functional connectivity offer for epileptic presurgical evaluation? Regarding this topic, this paper introduced the origin of MEG and the workflow of constructing source–space functional connectivity based on MEG signals. Resting-state functional connectivity abnormalities correlate with epileptogenic networks, which are defined by the brain regions involved in the production and propagation of epileptic activities. This paper reviewed the evidence of altered epileptic connectivity based on low- or high-frequency oscillations (HFOs) and the evidence of the advantage of using simultaneous MEG and intracranial electroencephalography (iEEG) recordings. More importantly, this review highlighted that MEG-based resting-state functional connectivity has the potential to predict postsurgical outcomes. In conclusion, resting-state MEG functional connectivity has made a substantial progress toward serving as a candidate biomarker included in epileptic presurgical evaluations.
Efficacy and Safety Study of Wearable Cyborg HAL (Hybrid Assistive Limb) in Hemiplegic Patients With Acute Stroke (EARLY GAIT Study): Protocols for a Randomized Controlled Trial
Authors: Watanabe Hiroki , Marushima Aiki , Kadone Hideki , Shimizu Yukiyo , Kubota Shigeki , Hino Tenyu , Sato Masayuki , Ito Yoshiro , Hayakawa Mikito , Tsurushima Hideo , Maruo Kazushi , Hada Yasushi , Ishikawa Eiichi , Matsumaru Yuji
Published: July 2021

We hypothesized that gait treatment with a wearable cyborg Hybrid Assistive Limb (HAL) would improve the walking ability of patients with hemiparesis after stroke. This study aims to evaluate the efficacy and safety of gait treatment using HAL versus conventional gait training (CGT) in hemiplegic patients with acute stroke and establish a protocol for doctor-initiated clinical trials for acute stroke. We will enroll patients with acute stroke at the University of Tsukuba Hospital. This study is a single-center, randomized, parallel-group, controlled trial (HAL group,n= 20; control group,n= 20) that will include three phases: (1) pre-observation phase (patient enrollment, baseline assessment, and randomization); (2) treatment phase (nine sessions, twice or thrice per week over 3−4 weeks; the HAL and control groups will perform gait treatment using HAL or CGT, respectively, and finally (3) post-treatment evaluation phase. The Functional Ambulation Category score will be the primary outcome measure, and the following secondary outcome measures will be assessed: Mini-Mental State Examination, Brunnstrom recovery stage of lower limbs, Fugl–Meyer assessment of lower limbs, 6-min walking distance, comfortable gait speed, step length, cadence, Barthel Index, Functional Independence Measure, gait posture, motion analysis (muscle activity), amount of activity (evaluated using an activity meter), stroke-specific QOL, and modified Rankin Scale score. The baseline assessment, post-treatment evaluation, and follow-up assessment will evaluate the overall outcome measures; for other evaluations, physical function evaluation centered on walking will be performed exclusively, excluding ADL and QOL scores. This study is a randomized controlled trial that aims to clarify the efficacy and safety of gait treatment using HAL compared with CGT in hemiplegic patients with acute stroke. In addition, we aim to establish a protocol for doctor-initiated clinical trials for acute stroke based on the study results. If our results demonstrate the effectiveness of the proposed treatment regarding outcomes of patients with hemiplegic acute stroke, this study will promote the treatment of these patients using the HAL system as an effective tool in future stroke rehabilitation programs. The study protocol was registered with the Japan Registry of Clinical Trials on October 14, 2020 (jRCTs032200151).
Pseudocirrhosis in Breast Cancer – Experience From an Academic Cancer Center
Authors: Gopalakrishnan Dharmesh , Shajihan Ain , Purysko Andrei S. , Abraham Jame
Published: July 2021

Background

Pseudocirrhosis is characterized by radiological changes in the liver that resemble cirrhosis, but with more rapid onset and progression. Though reported most frequently in patients with metastatic breast cancer, little is known about its prognostic factors and impact on breast cancer outcomes.

Methods

In this observational study, we reviewed abdominal CT and/or MRI scan reports of all patients with invasive breast cancer diagnosed at our center, during a ten-year period, to identify patients with pseudocirrhosis. Exclusion criteria included lack of baseline imaging, pre-existing cirrhosis, hepatitis B or C, other chronic liver diseases, or heavy alcohol use. Routine descriptive statistical measures were used. Survival distributions were estimated using Kaplan-Meier method, and Cox regression was used for multivariate analysis. Two-tailed p < 0.05 was considered significant.

Results

Eighty-six patients were included – all were females, median age was 57.5 years, and 90% were Caucasian; 86% of primary tumors were hormone-receptor positive and 17% were HER2 positive. Most patients (98%) had metastatic disease with liver involvement (94%), and were heavily pre-treated – 97% with chemotherapy, 85% with hormonal therapy, and 19% with anti-HER2 agents. Median interval from breast cancer diagnosis to pseudocirrhosis was 75.4 months (IQR 35.2-115.3 months). Thirty-six percentage of patients had ≥1 signs of portal hypertension and 49% had ≥1 signs of hepatocellular failure. Pseudocirrhosis led to permanent discontinuation of chemotherapy, endocrine therapy, and all systemic therapies in 29%, 31%, and 20% patients, respectively. Median overall survival from diagnosis of pseudocirrhosis was 10.0 months (95%CI 5.2-14.8 months). On multivariate analysis, coagulopathy, hyperbilirubinemia, hypoalbuminemia, and cancer progression were independently predictive of mortality.

Conclusions

In this largest series, to date, of breast cancer with pseudocirrhosis, the latter was often complicated by portal hypertension and hepatocellular failure, and markedly impacted breast cancer management. Survival was shorter for patients who developed hepatocellular failure.
Local Shape of the Vapor–Liquid Critical Point on the Thermodynamic Surface and the van der Waals Equation of State
Authors: Yu J. S. , Zhou X. , Chen J. F. , Du W. K. , Wang X. , Liu Q. H.
Published: July 2021

Differential geometry is a powerful tool to analyze the vapor–liquid critical point on the surface of the thermodynamic equation of state. The existence of usual condition of the critical point (∂p/∂V)T=0 requires the isothermal process, but the universality of the critical point is its independence of whatever process is taken, and so we can assume (∂p/∂T)V=0. The distinction between the critical point and other points on the surface leads us to further assume that the critical point is geometrically represented by zero Gaussian curvature. A slight extension of the van der Waals equation of state is to letting the two parameters a and b in it vary with temperature, which then satisfies both assumptions and reproduces its usual form when the temperature is approximately the critical one.
A Combined Pulse Driving Waveform With Rising Gradient for Improving the Aperture Ratio of Electrowetting Displays
Authors: Tian Lixia , Bai Pengfei
Published: July 2021

As a reflective display technology, electrowetting displays (EWDs) have the advantages of paper-like display, low power consumption, fast response, and full color, but the aperture ratio of EWDs is seriously affected by oil dispersion and charge trapping. In order to improve the aperture ratio and optimize the display performance of EWDs, a combined pulse driving waveform with rising gradient design was proposed. First, an initial driving voltage was established by the threshold voltage of oil film rupture (Vth). And then, a rising gradient was designed to prevent oil from dispersing. At last, the oil splitting and movement were controlled to achieve the target aperture combined with the pulse waveform. Experimental results showed that the oil dispersion of EWDs can be effectively improved by using the proposed driving waveform, the aperture ratio of EWDs was increased by 3.16%, and the stability was increased by 71.43%.
Drought and Nitrogen Application Modulate the Morphological and Physiological Responses of Dalbergia odorifera to Different Niche Neighbors
Authors: Xiang Li-Shan , Miao Ling-Feng , Yang Fan
Published: July 2021

Mixed stands can be more productive if growth facilitation via niche segregation occurs. Dalbergia odorifera T. Chen, a tropical tree species endemic to Hainan Island with great economic values, belongs to the family Leguminosae. However, selecting mixed species with suitable ecological niches to efficiently construct mixed forests of D. odorifera in the context of abiotic stress [drought, nitrogen (N) deposition] remained obscure. In the present study, the target plant D. odorifera was planted with the same species D. odorifera, heterogeneous but the same family Delonix regia and non-Leguminous Family Swietenia mahagoni in the root interaction and isolated models under two watering regimes [100% and 30% field capacity (FC)] and two N applications (application, non-application), respectively. Principle component analysis based on the performances of growth, phenotype, and physiology was performed to identify the main factors affected by the treatments and the most discriminatory effects of water, N level, and species interaction models. Both comprehensive evaluation values and comprehensive index values were calculated to evaluate the influences of different niche neighbors on D. odorifera. Results showed that D. odorifera was benefited from S. mahagoni but inhibited from D. odorifera in all treatments under root system interaction. Drought stress aggravated the inhibitory effects on D. odorifera from D. odorifera. N application stimulated the promoted effects on D. odorifera from S. mahagoni but enhanced competition intensity of D. odorifera from D. regia under the 100% FC condition. N application alleviated the inhibitory effect of drought stress on D. odorifera from D. odorifera and S. mahagoni. Furthermore, the responses of D. odorifera to different niche neighbors were dominated by belowground interaction rather than the negligible aboveground one. Therefore, the feasibility of niche segregation as the criterion for selecting neighbors to construct D. odorifera mixed stands was confirmed. In addition, water level and N application could alter responses of D. odorifera to different niche neighbors under the root system interaction. Appropriate N application could alleviate the inhibitory effect of drought stress on D. odorifera in its mixed forests. A mixture with S. mahagoni under appropriate N application could be the optimal planting model.
Influence of Psychological Need-Based Teachers’ Autonomy Support on Effectiveness and Engagement in English Learning
Authors: Liu Pengkai
Published: July 2021

It is to explore the effects of psychological needs and teachers’ autonomy support on students’ English learning, hoping to provide a realistic basis for the innovation of English teaching. Four hypotheses are proposed first, and 420 students are then randomly selected from seven classes in Xi’an Ai Zhi Zhong Xue in Shaanxi Province as the research subjects, with 420 questionnaires distributed. After invalid questionnaires are excluded, a total of 400 valid questionnaires are collected. Then, SPSS26.0 is employed to analyze the collected scale data. The average value of emotional exhaustion and the average value of low sense of achievement are relatively close in the direction of English learning burnout, which are larger than that in the other two directions. The three directions in English learning burnout are positively correlated with each other. The three directions in English learning engagement are positively correlated with those of psychological needs. Besides, there is an obvious positive correlation between the three directions of English learning engagement with teachers’ autonomy support (p < 0.01). However, the three directions in teachers’ autonomy support and the three directions in psychological needs are all negatively correlated with English learning burnout (p < 0.01). To sum up, the psychological needs of middle school students are effectively met, and teachers’ autonomy support can obviously promote the effectiveness and engagement of middle school students’ English learning.
Development and Evaluation of the CASTLE Trial Online Sleep Intervention for Parents of Children with Epilepsy
Authors: Wiggs Luci , Cook Georgia , Hiscock Harriet , Pal Deb K. , Gringras Paul
Published: July 2021

Introduction: Many of the sleep problems experienced by children with epilepsy (CWE) have the same behavioural basis as common sleep problems seen in typically developing (TD) children. Behavioural sleep interventions (BSIs) are widely used to treat these sleep problems in TD children and are hypothesised to be effective for CWE. However, specific considerations need to be addressed and incorporated into a BSI for CWE to ensure the intervention is tailored to this population's needs. This paper details developing and tailoring an online BSI for parents of CWE, to be used in the CASTLE (Changing Agendas on Sleep, Treatment and Learning in Epilepsy) Sleep-E clinical trial.

Method: In phase one, two existing theory-driven paediatric BSIs were adapted into a novel online behavioural sleep intervention (CASTLE Online Sleep Intervention or COSI) which specifically incorporated the needs and requirements reported by nine parents of CWE. Scoping their needs included conducting interviews with three CWE so that they could contribute to the overall intervention content. In phase two, six of these parents evaluated COSI, reviewing and feeding back on COSI until parental approval for content and functionality was achieved.

Results: In phase one, a range of adaptations was made to the content and presentation of standardised intervention material to acknowledge and emphasise the key seizure-specific issues to ensure COSI best met parents of CWE's needs. Adaptations included embedding parent and child experiences in the intervention, including particular information requested by parents, such as the links between sleep and seizures and managing child and parental anxieties around sleep, as well as developing functionality to personalise the delivery of content. In phase two, parents confirmed that they found the final version of COSI to be functional and appropriate (after one round of review) for use by parents of CWE and that 100% would recommend it to other families who have CWE.

Discussion: It is hoped that the use of evidence-based BSIs, adapted to consider salient epilepsy-specific factors, will increase parent-engagement, COSI's relevance for this particular patient group and overall efficacy in improving sleep in CWE. The effectiveness of COSI will be tested in the CASTLE Sleep-E clinical trial (https://castlestudy.org.uk/).
Impact of Acute and Chronic Cannabis Use on Stress Response Regulation: Challenging the Belief That Cannabis Is an Effective Method for Coping
Authors: al'Absi Mustafa , Allen Alicia M.
Published: July 2021

Although research has only recently started to examine the impact of cannabis use on stress response, there is some evidence that indicates acute and chronic impacts of cannabis on these processes. In this paper, we review processes involved in regulating the stress response and we review the influence of acute and chronic exposure to cannabis on patterns and regulation of the stress response. We also highlight the role of stress as a risk factor for initiation and maintenance of cannabis use. In this context, we examine moderating variables, including sex and life adversity. In light of recent observations indicating increasing prevalence of cannabis use during pregnancy, we provide additional focus on cannabis use in this vulnerable population, including how acute and chronic stress may predispose some individuals to use cannabis during pregnancy. While this line of research is in its infancy, we review available articles that focus on the perinatal period and that examined the association between cannabis use and various life stressors, including partner violence, job loss, and lack of housing. We also review psychiatric co-morbidities (e.g., post-traumatic stress disorder, anxiety). A better understanding of the way stress and cannabis use relate within the general population, as well as within certain subgroups that may be at a greater risk of using and/or at greater risk for adverse outcomes of use, may lead to the development of novel prevention and intervention approaches.
Origins of Dissociations in the English Past Tense: A Synthetic Brain Imaging Model
Authors: Westermann Gert , Jones Samuel
Published: July 2021

Brain imaging studies of English past tense inflection have found dissociations between regular and irregular verbs, but no coherent picture has emerged to explain how these dissociations arise. Here we use synthetic brain imaging on a neural network model to provide a mechanistic account of the origins of such dissociations. The model suggests that dissociations between regional activation patterns in verb inflection emerge in an adult processing system that has been shaped through experience-dependent structural brain development. Although these dissociations appear to be between regular and irregular verbs, they arise in the model from a combination of statistical properties including frequency, relationships to other verbs, and phonological complexity, without a causal role for regularity or semantics. These results are consistent with the notion that all inflections are produced in a single associative mechanism. The model generates predictions about the patterning of active brain regions for different verbs that can be tested in future imaging studies.
Better Later Than Never: Meaning in Late Life
Authors: Pachana Nancy A. , Baumeister Roy F.
Published: July 2021

The quest for meaning in life takes on new challenges and directions during late life. This mini-review draws on prior theory to analyze meaningfulness into six discrete dimensions (purpose, value, efficacy, self-worth, mattering, and comprehension) and covers research into how these apply and operate specifically during late life. Limited remaining time, concern with one's legacy, concerns with self-continuity and integration, variable challenges to self-worth, and prioritization of positivity emerge as key themes.
Preliminary Assessment of Chinese Strategy in Controlling Reemergent Local Outbreak of COVID-19
Authors: Wang Zhouhan , Jin Yanqi , Jin Xi , Lu Yingfeng , Yu Xiaopeng , Li Lanjuan , Zhang Yimin
Published: July 2021

Reemergent local outbreaks of coronavirus disease 2019 (COVID-19) have occurred in China, yet few Chinese response strategies and its evaluation have been reported. This study presents a preliminary assessment of Chinese strategy in controlling reemergent local outbreaks of COVID-19. Time course of accumulative and daily new cases and time-varying reproductive numbers (Rt) of outbreak areas were presented. The asymptomatic rate, days required to control the outbreaks, seeding time (ST), and doubling time (DT) of areas with over 96 reemergent cases were calculated. National and local year-on-year growth rates of gross domestic product (GDP) were presented. Accumulative numbers of 30, 8, 11, 430, 15, 139, 1,067, 382, 42, and 94 confirmed reemergent COVID-19 cases were diagnosed in Hulun Buir, Shanghai, Tianjin, Kashgar, Qingdao, Dalian, Urumchi, Beijing, Jilin, and Harbin, respectively. Among them, maximum rate of asymptomatic infections was 81.9%. Time required to control the local outbreaks in the areas given above varied from 29 to 51 days. After activation of outbreak responses, the late-stage DTs of Kashgar, Urumchi, Beijing, and Dalian were apparently lengthened compared to the early-stage DTs. Although the year-on-year GDP growth rate of Urumchi was slightly affected, the GDP growth rate of Dalian, Beijing, Jilin, and Harbin kept rising during the reemergence. Moreover, the year-on-year GDP growth rate of Mainland China turned positive regardless of the reemergent local outbreaks. In general, the Chinese strategy to maintain the status of no or minimal transmission was effective in balancing the control of COVID-19 reemergent local outbreak and the recovery of economy.
Insights Into the Older Adults' World: Concepts of Aging, Care, and Using Assistive Technology in Late Adulthood
Authors: Wilkowska Wiktoria , Offermann-van Heek Julia , Laurentius Thea , Bollheimer L. Cornelius , Ziefle Martina
Published: July 2021

The ongoing demographic change forces different stakeholders to cope with increasing needs in nursing care and the economic costs. Consequences arising from the population aging can be supported by assistive technologies to maintain older individuals' autonomy. However, older adults' opinions on the assistance of health-related technologies and their attitudes toward aging and care largely remain underexplored. This paper provides a geriatric and socio-technical perspective, investigating individual perceptions of (a) aging, (b) nursing care, and (c) the adoption of assistive technologies in a cross-national subject group. For this purpose, N = 384 individuals (60+ years) participated in an online survey. Findings indicate that most older adults are open to assistive technologies and that individual care preferences contribute to a successful adoption of these technologies. Among individual factors, health status, and gender affect respondents' opinions the most. Our findings help to understand older adults' acceptance of assistive technologies and contribute to the research on the nursing care in private and professional environments.
Care for Joy: Evaluation of a Humor Intervention and Its Effects on Stress, Flow Experience, Work Enjoyment, and Meaningfulness of Work
Authors: Bartzik Marek , Bentrup Andreas , Hill Susanne , Bley Maria , von Hirschhausen Eckart , Krause Gerrit , Ahaus Peter , Dahl-Dichmann Angelika , Peifer Corinna
Published: July 2021

The media increasingly speak of a care crisis. Systematic support is needed to prepare nursing apprentices for the high demands of their profession and to reduce the number of nurses who finally quit. Particularly in stressful jobs like nursing, humor as a coping strategy can have a beneficial effect on perceived stress and overall work enjoyment. In this study, we used a humor intervention among nursing staff in training and evaluated its effects on humor, stress, work enjoyment, the meaningfulness of work, and flow experience. The sample consists of 104 nurses in training. The intervention group received a 3-h humor intervention, while the control group received no intervention. Positive and negative affect were measured immediately before and after the intervention. Humor was measured before the intervention (t0) and again 6 months later (t1); at t1, we again measured humor and also stress, work meaningfulness, work enjoyment, and flow experience. Our analyses showed a beneficial change in positive and negative affect right after the intervention. By means of repeated measures ANOVA we could further confirm an effect of the intervention on reported humor 6 months later. Humor mediated positive effects of the humor intervention on perceived meaningfulness of work, work enjoyment, and on the frequency of flow at work. Also, we found a significant negative relationship between humor and stress measured at t1. The results of this study confirm the effectiveness of humor interventions in promoting humor, and, through this, the meaningfulness of work, work enjoyment, and the frequency of flow experience. Implications of the use of humor interventions in the nursing profession are discussed.