bluuebunny/crossref_metadata_2025 huggingface dataset:
Electric Vehicle Routing, Arc Routing, and Team Orienteering Problems in Sustainable Transportation
Authors: Martins Leandro do C. , Tordecilla Rafael D. , Castaneda Juliana , Juan Angel A. , Faulin Javier
Published: August 2021

The increasing use of electric vehicles in road and air transportation, especially in last-mile delivery and city mobility, raises new operational challenges due to the limited capacity of electric batteries. These limitations impose additional driving range constraints when optimizing the distribution and mobility plans. During the last years, several researchers from the Computer Science, Artificial Intelligence, and Operations Research communities have been developing optimization, simulation, and machine learning approaches that aim at generating efficient and sustainable routing plans for hybrid fleets, including both electric and internal combustion engine vehicles. After contextualizing the relevance of electric vehicles in promoting sustainable transportation practices, this paper reviews the existing work in the field of electric vehicle routing problems. In particular, we focus on articles related to the well-known vehicle routing, arc routing, and team orienteering problems. The review is followed by numerical examples that illustrate the gains that can be obtained by employing optimization methods in the aforementioned field. Finally, several research opportunities are highlighted.
Electrical Stress on the Medium Voltage Medium Frequency Transformer
Authors: Changjiang Zheng , Qian Wang , Huai Wang , Zhan Shen , Bak Claus Leth
Published: August 2021

This paper proposes an equivalent circuit model to obtain the transient electrical stress quantitatively in medium voltage medium frequency transformers in modern power electronics. To verify this model, transient simulation is performed on a 1.5 kV/1 kHz transformer, revealing voltage overshoot quantitatively between turns and layers of the transformer’s HV winding. Effects of rise time of the input pulse voltage, stray capacitance of the winding insulation, and their interactions on the voltage overshot magnitude are presented. With these results, we propose limiting the voltage overshoot and, thereafter, enhancing medium voltage medium frequency transformer’s insulation capability, which throws light on the transformer’s insulation design. Additionally, guidance on the future studies on aging and endurance lifetime of the medium voltage medium frequency transformer’s insulation could be given.
A New Solar Assisted Heat Pump System with Underground Energy Storage: Modelling and Optimisation
Authors: Ocłoń Paweł , Ławryńczuk Maciej , Czamara Marek
Published: August 2021

The objectives of this work are: (a) to present a new system for building heating which is based on underground energy storage, (b) to develop a mathematical model of the system, and (c) to optimise the energy performance of the system. The system includes Photovoltaic Thermal Hybrid Solar Panels (PVT) panels with cooling, an evacuated solar collector and a water-to-water heat pump. Additionally, storage tanks, placed underground, are used to store the waste heat from PVT panels cooling. The thermal energy produced by the solar collectors is used for both domestic hot water preparation and thermal energy storage. Both PVT panels and solar collectors are assembled with a sun-tracking system to achieve the highest possible solar energy gain. Optimisation of the proposed system is considered to achieve the highest Renewable Energy Sources (RES) share during the heating period. Because the resulting optimisation problem is nonlinear, the classical gradient-based optimisation algorithm gives solutions that are not satisfying. As alternatives, three heuristic global optimisation methods are considered: the Genetic Algorithm (GA), the Particle Swarm Optimisation (PSO) algorithm, and the Jaya algorithm. It is shown that the Jaya algorithm outperforms the GA and PSO methods. The most significant result is that 93% of thermal energy is covered by using the underground energy storage unit consisting of two tanks.
A Strategic and Smart Environmental Assessment of Rapid Urbanization in Beijing
Authors: Fiscal Paulina Rodríguez , Taratori Rallou , Pacho Marie Abigail , Ioakimidis Christos S. , Koutra Sesil
Published: August 2021

One of the key challenges of developing countries is to tackle the mitigation of the impacts of rapid and uncontrolled urbanization. Assessing this phenomenon is crucial to lessen the consequences for the environment and society. ‘Literature has been concentrated in planning strategies for the cities’ adaptation and engagements to the principles of green development ensuring a long-term quality of life for their citizens. Hereby, smart technologies and applications consist of two of the most encouraging concepts for solutions for achieving the 2030 and 2050 horizon targets towards clean energy transition and carbon neutrality. In academia, scholars have already raised the importance of ‘smartness’ to define the adaptative patterns for the global pressures of climate change and uncontrolled urban growth. The mitigation of these phenomena is crucial to ensure the cities’ future and lessen their impacts. This study seeks a strategic and smart-driven vision to leverage smartness on the phenomenon of rapid urbanization that occurred in the case of Beijing, China. Defining and evaluating the environmental impacts in line with the RIAM approach as one of its main targets. Future works can be focused on addressing solutions in similar cases in further developing countries to not only overcome environmental, but also economic, social, and digital complications.
Recent Development in the Design of Wind Deflectors for Vertical Axis Wind Turbine: A Review
Authors: Rajpar Altaf Hussain , Ali Imran , Eladwi Ahmad E. , Bashir Mohamed Bashir Ali
Published: August 2021

Developments in the design of wind turbines with augmentation are advancing around the globe with the goal of generating electricity close to the user in built-up areas. This is certain to help lessen the power generation load as well as distribution and transmission network costs by reducing the distance between the user and the power source. The main objectives driving the development and advancement of vertical-axis wind turbines are increasing the power coefficient and the torque coefficient by optimizing the upstream wind striking on the rotor blades. Unlike horizontal-axis wind turbines, vertical axis turbines generate not only positive torque but also negative torque during operation. The negative torque generated by the returning blade is a key issue for vertical-axis wind turbines (VAWTs) that is counterproductive. Installation of wind deflectors for flow augmentation helps to reduce the negative torque generated by the returning blades as well as enhance the positive torque by creating a diversion in the upstream wind towards the forwarding blade during operation. This paper reviews various designs, experiments, and CFD simulations of wind deflectors reported to date. Optimization techniques for VAWTs incorporating wind deflectors are discussed in detail. The main focus of the review was on the installation position and orientation of the deflectors and their potential contribution to increasing the power coefficient. Topics for future study are suggested in the conclusion section of the paper.
Comprehensive Modeling and Control of Grid-Connected Hybrid Energy Sources Using MPPT Controller
Authors: Mohamed Shazly A. , Tolba Mohamed A. , Eisa Ayman A. , El-Rifaie Ali M.
Published: August 2021

Nuclear and renewables energies are the two variants for low-carbon energy and the evolving grid suggests possible synergies between them. Nuclear energy introduces supple operations based on power demand, while renewables such as PV and wind hybrid systems depend on the presence and strength of sunlight or wind. For grid stability, there is need to improve their performance in order to overcome the impact of this disadvantage. The paper is a step in this direction as it addresses a detailed comprehensive dynamic modeling and an efficient control of grid-connected energy sources such as PV or wind system to increase system reliability and to ensure the power quality and safe operation of critical demands. The behavior of the suggested hybrid system is tested at different climate circumstances such as variation of sun radiation and wind speed. The PV is equipped with a boost converter and a three-phase pulse width modulation (PWM) inverter. The wind energy comprises a doubly fed generator (DFIG) based on a variable-speed wind turbine. The two controllers’ rotor-side and grid-side converters of DFIG have the ability to generate and observe reactive power, to keep constant speed of the rotor and control the DC-link voltage. The proposed scheme was investigated using MATLAB software. The maximum power point tracking (MPPT) was used for two systems, PV and wind, in varying weather conditions. The simulation results prove that the voltage at the point of common coupling was constant. Furthermore, the injected current of the grid side was in sinusoidal form and was synchronized with grid side voltage. In addition, the injected power-to-utility grid was around power delivered by the hybrid PV and wind system.
Evaluation of Methodology for Lightning Impulse Voltage Distribution over High-Voltage Windings of Inductive Voltage Transformers
Authors: Trkulja Bojan , Drandić Ana , Milardić Viktor , Žiger Igor
Published: August 2021

Knowledge of lightning impulse (LI) voltage distribution over transformer windings during the design stage of the transformer is very important. Specific design differences in inductive voltage transformers make the transient analysis approach different to the approach to the power transformers. In this paper, a methodology for acquiring lightning impulse voltage distribution over high-voltage (HV) winding of inductive voltage transformers is presented and evaluated. Resistance, inductance, and capacitance matrices are calculated using the integral and boundary element methods (BEM) approach. Additionally, in order to improve the capacitance matrix solver, adaptive cross approximation (ACA) is applied. These parameters are then used to solve the equivalent circuit model in time domain. In order to evaluate the methodology, an experimental and numerical investigation of the layer discretisation, iron core influence, and accuracy of the proposed methodology is performed. The comparison of numerical results with measurements confirms the validity of the methodology.
Nonlinear Optimal-Based Vibration Control of a Wind Turbine Tower Using Hybrid vs. Magnetorheological Tuned Vibration Absorber
Authors: Martynowicz Paweł
Published: August 2021

This paper presents an implementation of a nonlinear optimal-based wind turbine tower vibration control method. An NREL 5.0 MW tower-nacelle model equipped with a hybrid tuned vibration absorber (HTVA) is analysed against the model equipped with a magnetorheological TVA (MRTVA). For control purposes, a 3 kN active actuator in parallel with a passive TVA is used in the HTVA system, while an MR damper is built in the MRTVA instead of a viscous damper, as in a standard TVA. All actuator force constraints are embedded in the implemented nonlinear control techniques. By employing the Pontryagin maximum principle, the nonlinear optimal HTVA control proposition was derived along with its simplified revisions to avoid a high computational load during real-time control. The advantage of HTVA over MRTVA in vibration attenuation is evident within the first tower bending frequency neighbourhood, with HTVA also requiring less working space. Using the appropriate optimisation fields enabled an 8-fold reduction of HTVA energy demand along with a (further) 29% reduction of its working space while maintaining a significant advantage of HTVA over the passive TVA. The obtained results are encouraging for the assumed mass ratio and actuator force limitations, proving the effectiveness and validity of the proposed approaches.
Winding MMF and PM MMF Analysis of Axial-Flux Machine with Multi-Phase and Multi-Layer Winding
Authors: Chen Qixu , Li Guoli , Cao Wenping , Qian Zhe , Wang Qunjing
Published: August 2021

According to star graph and winding distribution, winding MMFs of three kinds of 12-slot/10-pole multi-phase and multi-layer winding layout are analyzed by the improved winding function method. Analysis results show that multi-phase and multi-layer winding can suppress even order and (12n ± 1) order harmonics, thereby reducing the eddy-current loss in PMs. Based on the unfolded LPMSM model, rotor MMF, air gap flux density, and no-load back-EMF are analyzed by the analytical permeance function method, which is validated by Teslameter and no-load experiment. Winding MMF is validated by FEM. An axial-flux integrated starting/generator (ISG) with double-three-phase four-layer (DTP-FL) winding, segmented armature and PM, and centrifugal fan is designed and manufactured. The no-load and load test with two groups of resistance established has validated the reasonability of the mentioned method. Experiments show that the axial-flux ISG prototype can run at a relative temperature rise.
Effects of Mass and Damping on Flow-Induced Vibration of a Cylinder Interacting with the Wake of Another Cylinder at High Reduced Velocities
Authors: Alam Md. Mahbub
Published: August 2021

Flow-induced vibration is a canonical issue in various engineering fields, leading to fatigue or immediate damage to structures. This paper numerically investigates flow-induced vibrations of a cylinder interacting with the wake of another cylinder at a Reynolds number Re = 150. It sheds light on the effects of mass ratio m*, damping ratio, and mass-damping ratio m*ζ on vibration amplitude ratio A/D at different reduced velocities Ur and cylinder spacing ratios L/D = 1.5 and 3.0. A couple of interesting observations are made. The m* has a greater influence on A/D than ζ although both m* and ζ cause reductions in A/D. The m* effect on A/D is strong for m* = 2–16 but weak for m* > 16. As opposed to a single isolated cylinder case, the mass-damping m*ζ is not found to be a unique parameter for a cylinder oscillating in a wake. The vortices in the wake decay rapidly at small ζ. Alternate reattachment of the gap shear layers on the wake cylinder fuels the vibration of the wake cylinder for L/D = 1.5 while the impingement and switch of the gap vortices do the same for L/D = 3.0.
An Optimal Train Speed Profile Planning Method for Induction Motor Traction System
Authors: Wu Ziyu , Gao Chunhai , Tang Tao
Published: August 2021

Optimizing the operating speed curve of trains without adding new energy storage facilities is essential in the energy-saving operation of railways. In this paper, we propose an optimal train speed curve planning method for driving trains more energy efficiently. A refined traction energy evaluation model for induction motor propulsion systems is first presented. The proposed model considers the efficiency of the traction motor at different operating points and the efficiency of the inverter and gearbox. Then, the optimal energy-efficient speed profile problem is transformed into a multistep decision problem and solved using dynamic programming (DP). To verify the effectiveness of the proposed method, a case study was conducted on an actual subway line. The results obtained indicate that the speed curve produced by the proposed method results in a 20% energy consumption saving compared with the speed curve for actual operations. Furthermore, the results of comparison with a genetic algorithm indicate that the DP algorithm is better able to satisfy the constraints of the train traction system. Solving the optimal speed curve using the proposed method and programming the onboard controller of the train according to the optimal speed curve enables the train to be driven with greater energy efficiency.
An Efficient HVAC Network Control for Safety Enhancement of a Typical Uninterrupted Power Supply Battery Storage Room
Authors: Lencwe Mpho J. , Chowdhury SP Daniel , Mahlangu Sipho , Sibanyoni Maxwell , Ngoma Louwrance
Published: August 2021

Lead-acid batteries utilised in electrical substations release hydrogen and oxygen when these are charged. These gases could be dangerous and cause a risk of fire if they are not properly ventilated. Therefore, this research seeks to design and implement a network control panel for heating, ventilation, and air conditioning system (HVACS). This is achieved by using a specific range of controllers, which have more than thirty loops of proportional, integral, and derivative (PID) control to achieve a cost-effective design. It performs the required function of extracting hydrogen and oxygen, maintaining the desired temperature of the battery storage room within recommended limits (i.e., 25 ± 1 °C tolerance) without compromising quality, as set out in the user requirement specification. The system control panel allows the user to access control parameters such as changing temperature set-points, fan-speed, sensor database, etc. It does this automatically and allows no human interface after all necessary settings and installation are completed. The hardware is configured to detect extreme hydrogen and oxygen gas content in the battery room and to ensure that the HVACS extracts the gas content to the outside environment. The system’s results show that the network control panel operates effectively as per the recommended system requirements. Therefore, the effective operation of the HVACS ensures sufficient gas ventilation, thus mitigating the risk of fire in a typical battery storage room. Furthermore, this also enhances battery lifespan because of regulated operating temperature, which is conducive to minimise the effect of sulfation in lead–acid batteries (LAB). The extraction of toxic gases, regulation of temperature, ensuring suitable humidity in UPS battery room is important as it provides longer operational service of equipment, thus reducing frequent maintenance in these rooms. This benefits the electricity supply industry and helps in saving for unplanned maintenance costs.
Design Optimization of Flexure Springs for Free-Piston Stirling Engines and Experimental Evaluations with Fatigue Testing
Authors: Lee Chang-Whan , Kim Dong-Jun , Kim Sung-Kwon , Sim Kyuho
Published: August 2021

The free-piston Stirling engine is a closed-cycle regenerative heat engine that converts heat energy into mechanical work, and requires a spring element for vibratory operations of the displacer and power pistons. In this study, the geometry of the flexural spring design was optimized through structural finite element analyses and fatigue test evaluations. First, we constructed a target design space considering the required natural frequency of the displacer spring assembly under the geometric constraints of total mass and module height. The design of experiments was employed to construct simulation cases for design factors such as the outer diameter, thickness, and number of spirals in the spring sheet. As a result, the optimized design values were obtained to satisfy the design requirements. We also fabricated a test spring specimen and conducted fatigue tests using a linear actuator system developed to have the same motion as the engine. The test results indicated that the optimized spiral spring had no fracture under operating conditions with the design piston amplitude, revealing the effectiveness of the design method.
Preparation and Directed Evolution of Anti-Ciprofloxacin ScFv for Immunoassay in Animal-Derived Food
Authors: Wang Fangyu , Li Ning , Zhang Yunshang , Sun Xuefeng , Hu Man , Zhao Yali , Fan Jianming
Published: August 2021

An immunized mouse phage display scFv library with a capacity of 3.34 × 109 CFU/mL was constructed and used for screening of recombinant anti-ciprofloxacin single-chain antibody for the detection of ciprofloxacin (CIP) in animal-derived food. After four rounds of bio-panning, 25 positives were isolated and identified successfully. The highest positive scFv-22 was expressed in E. coli BL21. Then, its recognition mechanisms were studied using the molecular docking method. The result showed the amino acid residue Val160 was the key residue for the binding of scFv to CIP. Based on the results of virtual mutation, the scFv antibody was evolved by directional mutagenesis of contact amino acid residue Val160 to Ser. After the expression and purification, an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) based on the parental and mutant scFv was established for CIP, respectively. The IC50 value of the assay established with the ScFv mutant was 1.58 ng/mL, while the parental scFv was 26.23 ng/mL; this result showed highly increased affinity, with up to 16.6-fold improved sensitivity. The mean recovery for CIP ranged from 73.80% to 123.35%, with 10.46% relative standard deviation between the intra-assay and the inter-assay. The RSD values ranged between 1.49% and 9.81%. The results indicate that we obtained a highly sensitive anti-CIP scFv by the phage library construction and directional evolution, and the scFv-based IC-ELISA is suitable for the detection of CIP residue in animal-derived edible tissues.
Food Safety of Consuming Black Soldier Fly (Hermetia illucens) Larvae: Microbial, Heavy Metal and Cross-Reactive Allergen Risks
Authors: Bessa Leah W. , Pieterse Elsje , Marais Jeannine , Dhanani Karim , Hoffman Louwrens C.
Published: August 2021

Black soldier fly (Hermetia illucens) larvae (BSFL) are a promising, sustainable source of nutrients, however, there is limited knowledge regarding the food safety of consuming BSFL. This study determined the safety of consuming BSFL for direct human consumption in terms of microbial, heavy metal and allergen content. Microbial counts were determined using ISO (International Organization for Standardization) methods, heavy metals were determined using inductively coupled plasma mass spectrometry and allergens were determined via Orbitrap mass spectrometry and ELISA (enzyme-linked immunosorbent assay) kits. Feed and killing method influenced the presence of Bacillus cereus (p = 0.011), and only the killing method influenced Escherichia coli (p < 0.00) and total viable count (TVC) (p < 0.00). Blanching resulted in a 3-log reduction in E. coli and a 3.4 log reduction in the TVC counts. Salmonella spp. and Listeria spp. were not detected in the BSFL samples. Heavy metals were detected although they were below maximum legal limits. Cross-reactive allergens, tropomyosin and arginine kinase, were detected in the BSFL samples, although the clinical significance requires research. The feed fed to the BSFL and blanching were found to influence the safety of consuming BSFL, highlighting the importance of incorporating sufficient decontamination steps, such as blanching, to ensure food safety.
Application of Response Surface Methodology Based on a Box-Behnken Design to Determine Optimal Parameters to Produce Brined Cabbage Used in Kimchi
Authors: Song Hyeyeon , Moon Eun-woo , Ha Ji-Hyoung
Published: August 2021

The factors of brine time, concentration, and temperature, affect the high-quality production of brined cabbage used in Kimchi. Although changes in Kimchi cabbage quality depending on brine time and concentration have been reported, changes in brine temperature have not been explored. Here, we optimized the brine process considering specific conditions of temperature (15–25 °C), concentration (10–14%), and osmosis duration (14–18 h) affecting the characteristics such as pH, titratable acidity, soluble solid content, glucose, fructose, and lactic acid bacteria and mass transport (salt gain, water loss, and weight reduction). The optimal parameters were determined using multivariate statistical analysis using the Box–Behnken design combined with response surface methodology. For each response as qualitative characteristics, second order polynomial models were developed using multiple regression analysis. Analysis of variance was performed to check the adequacy and accuracy of the fitted models. The brine temperature and concentration affected salt gain and water loss; the optimal brining temperature, concentration, and time were 19.17 °C, 10.53%, and 15.38 h, respectively. Statistical regression analysis indicated that standardized brined cabbage can be produced efficiently using a brining tank at controllable temperature.
Sensory and Physical Characteristics of M. biceps femoris from Older Cows Using Ginger Powder (Zingibain) and Sous Vide Cooking
Authors: Naqvi Zahra B. , Thomson Peter C. , Campbell Michael A. , Latif Sajid , Legako Jerrad F. , McGill David M. , Wynn Peter C. , Friend Michael A. , Warner Robyn D.
Published: August 2021

This study aimed to evaluate the sensory and physical characteristics of zingibain-injected meat combined with sous vide cooking. M. biceps femoris (BF; n = 12) acquired from 6–7 year old Angus cows were cooked using the sous vide method at 65 °C, for 8 h or 12 h, either with ginger powder (GP) injected in a 2 g/L solution in water (treatment) or un-injected (control). The sensory attributes included flavour, juiciness, tenderness, and physicochemical characteristics were Warner-Bratzler shear (WBSF), hardness, total water content (TWC), cooking loss (CL) and collagen content. A significant improvement in tenderness with injection treatment and cooking time was observed, as evaluated through trained sensory panellists, and reduced WBSF and hardness (p < 0.05 for all). The flavour of the meat was not affected by injection treatment or cooking time (p > 0.05), but juiciness and TWC were reduced with longer cooking times (p < 0.01 for both). Soluble collagen increased with injection treatment and cooking time (both p < 0.05). Moderate to high correlations were found between sensory and physical measurements for tenderness and juiciness. The longer cooking time (12 h) with GP injection treatment caused over tenderization of the meat. The soft texture associated with over-tenderization may be suitable for some specialised consumer markets, for instance, the elderly population with chewing difficulties. Improving the eating quality of low-quality meat from old animals through sous vide cooking and the use of ginger proteases may increase the acceptability of lower value beef, potentially enhancing the commercial value of carcasses typically produced in the beef industry.
Investigation of Photoprotective, Anti-Inflammatory, Antioxidant Capacities and LC–ESI–MS Phenolic Profile of Astragalus gombiformis Pomel
Authors: Lekmine Sabrina , Boussekine Samira , Akkal Salah , Martín-García Antonio Ignacio , Boumegoura Ali , Kadi Kenza , Djeghim Hanene , Mekersi Nawal , Bendjedid Samira , Bensouici Chawki , Nieto Gema
Published: August 2021

Plant-derived compounds have recently been gaining popularity as skincare factors due to their ability to absorb ultraviolet radiations and their anti-inflammatory, and antioxidant properties. In this light, this work aimed to evaluate in vitro the pharmacological activities of the butanolic extract prepared from the aerial parts of Astragalus gombiformis Pomel, an endemic species to southern Algeria. The sun protection factor was used to assess the photoprotective effect (SPF), the protein denaturation method to determine the anti-inflammatory activity, and brine shrimp nauplii and OxHLIA assay, respectively, to assess the cytotoxicity and antioxidant capacity of A. gombiformis. In addition, LC–ESI–MS analysis was employed for the characterization of the phenolic constituents of A. gombiformis. The results showed that A. gombiformis had high capacity for absorbing UV radiations with an SPF of 37.78 ± 0.85 and significant anti-inflammatory activity with a percentage inhibition of 75.38% which is close to that of diclofenac and ketoprofen. In addition, A. gombiformis was found to have effective cytotoxicity against Artemia nauplii with a DC50 value of about 44.7 µg/mL, but a weak hemolytic effect against human erythrocytes. LC–ESI–MS results detected the presence of 17 phenolic compounds with a predominance of cirsiliol, silymarin, quercitrin (quercetin-3-O-rhamnoside), and kaempferol. Taken together, these results suggest that A. gombiformis extract could be used as a skincare agent in cosmetic formulations, providing excellent antioxidant and anti-inflammatory protection, allowing the treatment of skin conditions, as well as a pharmaceutical agent with multidimensional applications.
Dynamic In Vitro Gastric Digestion of Sheep Milk: Influence of Homogenization and Heat Treatment
Authors: Pan Zheng , Ye Aiqian , Li Siqi , Dave Anant , Fraser Karl , Singh Harjinder
Published: August 2021

Milk is commonly exposed to processing including homogenization and thermal treatment before consumption, and this processing could have an impact on its digestion behavior in the stomach. In this study, we investigated the in vitro gastric digestion behavior of differently processed sheep milks. The samples were raw, pasteurized (75 °C/15 s), homogenized (200/20 bar at 65 °C)–pasteurized, and homogenized–heated (95 °C/5 min) milks. The digestion was performed using a dynamic in vitro gastric digestion system, the human gastric simulator with simulated gastric fluid without gastric lipase. The pH, structure, and composition of the milks in the stomach and the emptied digesta, and the rate of protein hydrolysis were examined. Curds formed from homogenized and heated milk had much looser and more fragmented structures than those formed from unhomogenized milk; this accelerated the curd breakdown, protein digestion and promoted the release of protein, fat, and calcium from the curds into the digesta. Coalescence and flocculation of fat globules were observed during gastric digestion, and most of the fat globules were incorporated into the emptied protein/peptide particles in the homogenized milks. The study provides a better understanding of the gastric emptying and digestion of processed sheep milk under in vitro gastric conditions.
Recent Applications of Mixture Designs in Beverages, Foods, and Pharmaceutical Health: A Systematic Review and Meta-Analysis
Authors: Galvan Diego , Effting Luciane , Cremasco Hágata , Conte-Junior Carlos Adam
Published: August 2021

Design of Experiments (DoE) is a statistical tool used to plan and optimize experiments and is seen as a quality technology to achieve products excellence. Among the experimental designs (EDs), the mixture designs (MDs) stand out, being widely applied to improve conditions for processing, developing, or formulating novel products. This review aims to provide useful updated information on the capacity and diversity of MDs applications for the industry and scientific community in the areas of food, beverage, and pharmaceutical health. Recent works were selected following the Preferred Reporting Items for Systematic Review and Meta-Analyses statement (PRISMA) flow diagram. Data analysis was performed by self-organizing map (SOM) to check and understand which fields of application/countries/continents are using MDs. Overall, the SOM indicated that Brazil presented the largest number of works using MDs. Among the continents, America and Asia showed a predominance in applications with the same amount of work. Comparing the MDs application areas, the analysis indicated that works are prevalent in food and beverage science in the American continent, while in Asia, health science prevails. MDs were more used to develop functional/nutraceutical products and the formulation of drugs for several diseases. However, we briefly describe some promising research fields in that MDs can still be employed.
Steam Explosion-Assisted Extraction of Protein from Fish Backbones and Effect of Enzymatic Hydrolysis on the Extracts
Authors: Dong Ye , Yan Wen , Zhang Xiao-Di , Dai Zhi-Yuan , Zhang Yi-Qi
Published: August 2021

The development of an efficient pretreatment, prior to enzymatic hydrolysis, is a good strategy for the sustainable use of refractory fish byproducts. This study compared hydrothermal pretreatments at 159 °C for 2 min, followed by water extraction (steam explosion-assisted extraction, SE) and 121 °C for 70 min (hot-pressure extraction, HPE), for the recovery of proteins from fish backbones. The effect of enzymatic hydrolysis on the properties of the obtained fish bone protein (FBP) was also evaluated. The results demonstrated that FBP had high contents of protein (81.09–84.88 g/100 g) and hydroxyproline (70–82 residues/1000 residues). After hydrolysis with Flavourzyme, for 3 h, the FBP hydrolysates that were pretreated with SE (SFBP-H) exhibited a better degree of hydrolysis (DH) and nitrogen recovery (NR), and a higher level of umami taste free amino acids (151.50 mg/100 mL), compared with the HPE-treated samples. The obtained SFBP-H mainly distributed below 3000 Da and had strong scavenging effects on 1,1-diphenyl-2-picrylhydrazy (DPPH) (IC50 = 4.24 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC50 = 1.93 mg/mL) radicals. Steam explosion-assisted extraction is a promising route for recovering proteins from native fish bone materials, and improving the flavor and antioxidant activity of the hydrolysates.
Complex Interactions in Regulation of Haematopoiesis—An Unexplored Iron Mine
Authors: De Ranita , Prakash Kulkarni Uday , Edison Eunice S.
Published: August 2021

Iron is one of the most abundant metals on earth and is vital for the growth and survival of life forms. It is crucial for the functioning of plants and animals as it is an integral component of the photosynthetic apparatus and innumerable proteins and enzymes. It plays a pivotal role in haematopoiesis and affects the development and differentiation of different haematopoietic lineages, apart from its obvious necessity in erythropoiesis. A large amount of iron stores in humans is diverted towards the latter process, as iron is an indispensable component of haemoglobin. This review summarises the important players of iron metabolism and homeostasis that have been discovered in recent years and highlights the overall significance of iron in haematopoiesis. Its role in maintenance of haematopoietic stem cells, influence on differentiation of varied haematopoietic lineages and consequences of iron deficiency/overloading on development and maturation of different groups of haematopoietic cells have been discussed.
Clinical Relevance of VEGFA (rs3025039) +936 C>T Polymorphism in Primary Myelofibrosis: Susceptibility, Clinical Co-Variates, and Outcomes
Authors: Villani Laura , Carolei Adriana , Rosti Vittorio , Massa Margherita , Campanelli Rita , Catarsi Paolo , Abbà Carlotta , Gale Robert Peter , Barosi Giovanni
Published: August 2021

We evaluated the association of VEGFA rs3025039 polymorphism with clinical co-variates and outcomes in 849 subjects with primary myelofibrosis (PMF) and 250 healthy controls. Minor T-allele frequency was higher in subjects with JAK2V617F compared with those without JAK2V617F (18% vs. 13%; p = 0.014). In subjects with JAK2V617F, the TT genotype was associated at diagnosis with lower platelet concentrations (p = 0.033), higher plasma LDH concentration (p = 0.005), higher blood CD34-positive cells (p = 0.027), lower plasma cholesterol concentration (p = 0.046), and higher concentration of high-sensitivity C-reactive protein (p = 0.018). These associations were not found in subjects with PMF without JAK2V617F. In subjects with the TT genotype, risk of death was higher compared with subjects with CC/CT genotypes (HR = 2.12 [1.03, 4.35], p = 0.041). Finally, the TT genotype was associated with higher frequency of deep vein thrombosis in typical sites (12.5% vs. 2.5%; OR = 5.46 [1.51, 19.7], p = 0.009). In conclusion, in subjects with PMF, the VEGFA rs3025039 CT or TT genotypes are more common in those with JAK2V617F than in those without JAK2V67F mutation and are associated with disease severity, poor prognosis, and risk of deep vein thrombosis.
Alternative Splicing and Hypoxia Puzzle in Alzheimer’s and Parkinson’s Diseases
Authors: Jakubauskienė Eglė , Kanopka Arvydas
Published: August 2021

Alternative pre-mRNA splicing plays a very important role in expanding protein diversity as it generates numerous transcripts from a single protein-coding gene. Therefore, alterations lead this process to neurological human disorders, including Alzheimer’s and Parkinson’s diseases. Moreover, accumulating evidence indicates that the splicing machinery highly contributes to the cells’ ability to adapt to different altered cellular microenvironments, such as hypoxia. Hypoxia is known to have an effect on the expression of proteins involved in a multiple of biological processes, such as erythropoiesis, angiogenesis, and neurogenesis, and is one of the important risk factors in neuropathogenesis. In this review, we discuss the current knowledge of alternatively spliced genes, which, as it is reported, are associated with Alzheimer’s and Parkinson’s diseases. Additionally, we highlight the possible influence of cellular hypoxic microenvironment for the formation of mRNA isoforms contributing to the development of these neurodegenerative diseases.
Impact of External Environmental Dimensions on Financial Performance of Major Teaching Hospitals in the U.S.
Authors: Lalani Karima , Revere Lee , Chan Wenyaw , Champagne-Langabeer Tiffany , Tektiridis Jennifer , Langabeer James
Published: August 2021

Teaching hospitals have a unique mission to not only deliver graduate medical education but to also provide both inpatient and ambulatory care and to conduct clinical medical research; therefore, they are under constant financial pressure, and it is important to explore what types of external environmental components affect their financial performance. This study examined if there is an association between the short-term and long-term financial performance of major teaching hospitals in the United States and the external environmental dimensions, as measured by the Resource Dependence Theory. Data for 226 major teaching hospitals spanning 46 states were analyzed. The dependent variable for short-term financial performance was days cash on hand, and dependent variable for long-term financial performance was return on assets, both an average of most recently available 4-year data (2014–2017). Utilizing linear regression model, results showed significance between outpatient revenue and days cash on hand as well as significant relationship between population of the metropolitan statistical area, unemployment rate of the metropolitan statistical area, and teaching hospital’s return on assets. Additionally, system membership, type of ownership/control, and teaching intensity also showed significant association with return on assets. By comprehensively examining all major teaching hospitals in the U.S. and analyzing the association between their short-term and long-term financial performance and external environmental dimensions, based upon Resource Dependence Theory, we found that by offering diverse outpatient services and novel delivery options, administrators of teaching hospitals may be able to increase organizational liquidity.
Serum Inflammatory and Prooxidant Marker Levels in Different Periodontal Disease Stages
Authors: Andreu Ricardo , Santos-del-Riego Sergio , Payri Francisco
Published: August 2021

Background: Periodontitis has been associated to systemic diseases and this association could be due to an increase in circulating inflammatory and oxidative stress biomarkers in the periodontal disease. This study aimed to evaluate the relationship between inflammatory and pro-oxidant markers according to different stages of periodontitis. Methods: This cross-sectional study included 70 subjects who were divided into three groups according to periodontitis stage: stage II (n = 22), stage III (n = 30), and stage IV (n = 18). We evaluated periodontal parameters and levels of high-sensitivity C-reactive protein (hsCRP), fibrinogen, and malondialdehyde (MDA) in serum, and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in urine. Results: Serum hsCRP and fibrinogen levels were associated with periodontitis severity, which were higher in stage IV than in stages III and II of periodontitis (p = 0.003 and p = 0.025, respectively). We observed a slight yet insignificant increase in MDA levels related to periodontitis severity. Probing depth and clinical attachment loss were associated with serum fibrinogen and hsCRP levels. However, there were no significant associations between periodontal variables and MDA and 8-OHdG levels. Conclusion: Our data support an association between periodontitis and systemic inflammation, which increases with periodontal disease severity. This indicates the importance of the early diagnosis and treatment of periodontal disease to avoid the development or worsening of systemic inflammatory diseases.
Effect of Ranitidine Intake on the Risk of Gastric Cancer Development
Authors: Kim SunMoon , Lee Suehyun , Hong JeeYoung , Ko Inseok , Kim Jong-Yeup , Kim Dong-Kyu
Published: August 2021

Gastric cancer is one of the most prevalent cancers globally, with high mortality, particularly in East Asia. Certain ranitidine products contain potentially carcinogenic N-nitrosodimethylamine. We investigated the potential association between gastric cancer risk and ranitidine intake using a nationwide cohort, extracted from the Korean National Health Insurance Service. In this longitudinal study, we employed a 1:1 propensity score matching according to sociodemographic factors. A total of 40,887 subjects were enrolled, of which 906 developed gastric cancer during the follow-up period. We investigated gastric cancer events during the follow-up period using the survival analysis, log-rank test, and Cox proportional hazards regression models to estimate incidence, survival rate, and hazard ratio. The incidence of gastric cancer was 67,422; 67,470; and 67,444 person-years in the control, other histamine-2 blockers, and ranitidine groups, respectively. Because the adjusted hazard ratio of gastric cancer was 0.98 and 1.01 in the other histamine-2 blockers and ranitidine groups, respectively, we could not calculate the likelihood of gastric cancer development in the ranitidine group. Ranitidine intake did not significantly increase the incidence of gastric cancer. Therefore, the relative risk of gastric cancer may be low in patients taking ranitidine products in South Korea.
Designing for Effective and Safe Multidisciplinary Primary Care Teamwork: Using the Time of COVID-19 as a Case Study
Authors: Lim Lisa , Zimring Craig M. , DuBose Jennifer R. , Lee Jaehoon , Stroebel Robert J. , Matthews Marc R.
Published: August 2021

Effective medical teamwork can improve the effectiveness and experience of care for staff and patients, including safety. Healthcare organizations, and especially primary care clinics, have sought to improve medical teamwork through improved layout and design, moving staff into shared multidisciplinary team rooms. While co-locating staff has been shown to increase communication, successful designs balance four teamwork needs: face-to-face communications; situational awareness; heads-down work; perception of teamness. However, precautions for COVID-19 make it more difficult to conduct face-to-face communications. In this paper we describe a model for understanding how layout affects these four teamwork needs and describe how the perception of teamwork by staff changed after COVID-19 precautions were put in place. Observations, interviews and two standard surveys were conducted in two primary care clinics before COVID-19 and again in 2021 after a year of precautions. In general, staff felt more isolated and found it more difficult to conduct brief consults, though these perceptions varied by role. RNs, who spent more time on the phone, found it convenient to work part time-from home, while medical assistants found it more difficult to find providers in the distanced clinics. These cases suggest some important considerations for future clinic designs, including greater physical transparency that also allow for physical separation and more spaces for informal communication that are distanced from workstations.
Participatory Development and Preliminary Psychometric Properties of the User-Friendly Patient Information Material Checklist (UPIM-Check)
Authors: Salm Sandra , Mollenhauer Judith , Hornbach Carolin , Cecon Natalia , Dresen Antje , Houwaart Stefanie , Arning Anna , Göttel Andrea , Schwickerath Kathrin , Pfaff Holger , Scholten Nadine , Krieger Theresia
Published: August 2021

The aims of this study were (1) to design a user-friendly instrument to assess and optimize patient information material (PIM), (2) to develop an English version, and (3) to test its psychometric properties. The instrument was needed to optimize the top-down developed PIM of the psycho-oncological care programme isPO. First, a literature-based PIM checklist was developed by a team of patient representatives, cancer care experts and professional researchers. Next, the checklist’s reliability and validity were analysed by having cancer survivors assess the initial and optimized version of the isPO-leaflet. The User-friendly Patient Information Material Checklist (UPIM-Check), developed participatorily, was found to be effective for evaluating PIM. It uses a traffic light scale, and suggestions for improvement can be given for each criterion. Its reliability appeared to be excellent (α = 0.927). The optimized leaflet was rated significantly better than the initial one. The UPIM-Check is a reliable and valid instrument, which enables end-users (e.g., patients) to assess and optimize the quality of PIM according to scientific criteria and the needs of end-users. A bottom-up approach was essential for developing and validating the UPIM-Check. End-users constantly contributed with their specific knowledge. Thus, their position as co-researchers was significantly strengthened.
Experiences of Changes in Eating Habits and Eating Behaviors of Women First Diagnosed with Gestational Diabetes
Authors: Kim Hye-Jin , Cho Eunjeong , Shin Gisoo
Published: August 2021

As gestational diabetes, which is increasing steadily around the world, can cause complications in the mother and fetus, it is essential to change eating habits and eating behavior to prevent this. According to the 2020 American Diabetes Association recommendations, the food plan should be designed for the adequate calorie intake to achieve glycemic goals and consequently promote maternal and fetal health. Thus, the following study has used the qualitative theme analysis method to assess what it means for 28 South Korean women, who were diagnosed with gestational diabetes for the first time, to change their eating habits and behaviors. As a result, themes were derived related to reflection on daily life, formation of new relationships in the same group, efforts that must be made, rediscovery of couples, and lifestyles reborn as new roles. Based on the results of the study, it is shown that the study participants recovered the peace in their mental state after the crisis of gestational diabetes to pursue relaxation and ultimately higher quality of life by following the plan to fulfill healthy achievements, such as changing their eating habits and behaviors. Therefore, future research and support measures to help the healthy behaviors should be sought by comprehensively exploring the effects of women’s experiences in changing their eating habits and behaviors.