bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Comparative In Vitro and In Vivo Study of BNN27 Mucoadhesive Liposomes and Nanoemulsions for Nose-to-Brain Delivery
Authors: Kannavou Maria , Karali Kanelina , Katsila Theodora , Siapi Eleni , Marazioti Antonia , Klepetsanis Pavlos , Calogeropoulou Theodora , Charalampopoulos Ioannis , Antimisiaris Sophia G.
Published: January 2023

Intranasal administration offers an alternative and promising approach for direct nose-to-brain delivery. Herein, we developed two chitosan (CHT)-coated (and uncoated) nanoformulations of BNN27 (a synthetic C-17-spiro-dehydroepiandrosterone analogue), liposomes (LIPs), and nanoemulsions (NEs), and compared their properties and brain disposition (in vitro and in vivo). LIPs were formulated by thin film hydration and coated with CHT by dropwise addition. BNN27-loaded NEs (BNEs) were developed by spontaneous emulsification and optimized for stability and mucoadhesive properties. Mucoadhesive properties were evaluated by mucin adherence. Negatively charged CHT-coated LIPs (with 0.1% CHT/lipid) demonstrated the highest coating efficiency and mucoadhesion. BNEs containing 10% w/w Capmul-MCM and 0.3% w/w CHT demonstrated the optimal properties. Transport of LIP or NE-associated rhodamine-lipid across the blood–brain barrier (in vitro) was significantly higher for NEs compared to LIPs, and the CHT coating demonstrated a negative effect on transport. However, the CHT-coated BNEs demonstrated higher and faster in vivo brain disposition following intranasal administration compared to CHT-LIPs. For both BNEs and LIPs, CHT-coating resulted in the increased (in vivo) brain disposition of BNN27. Current results prove that CHT-coated NEs consisting of compatible nasal administration ingredients succeeded in to delivering more BNN27 to the brain (and faster) compared to the CHT-coated LIPs.
Trioleyl Pyridinium, a Cationic Transfection Agent for the Lipofection of Therapeutic Oligonucleotides into Mammalian Cells
Authors: Delgado Ana , Griera Rosa , Llor Núria , López-Aguilar Ester , Busquets Maria Antònia , Noé Véronique , Ciudad Carlos J.
Published: January 2023

Background: One of the most significant limitations that therapeutic oligonucleotides present is the development of specific and efficient delivery vectors for the internalization of nucleic acids into cells. Therefore, there is a need for the development of new transfection agents that ensure a proper and efficient delivery into mammalian cells. Methods: We describe the synthesis of 1,3,5-tris[(4-oelyl-1-pyridinio)methyl]benzene tribromide (TROPY) and proceeded to the validation of its binding capacity toward oligonucleotides, the internalization of DNA into the cells, the effect on cell viability, apoptosis, and its capability to transfect plasmid DNA. Results: The synthesis and chemical characterization of TROPY, which can bind DNA and transfect oligonucleotides into mammalian cells through clathrin and caveolin-mediated endocytosis, are described. Using a PPRH against the antiapoptotic survivin gene as a model, we validated that the complex TROPY–PPRH decreased cell viability in human cancer cells, increased apoptosis, and reduced survivin mRNA and protein levels. TROPY was also able to stably transfect plasmid DNA, as demonstrated by the formation of viable colonies upon the transfection of a dhfr minigene into dhfr-negative cells and the subsequent metabolic selection. Conclusions: TROPY is an efficient transfecting agent that allows the delivery of therapeutic oligonucleotides, such as PPRHs and plasmid DNA, inside mammalian cells.
CSM-Toxin: A Web-Server for Predicting Protein Toxicity
Authors: Morozov Vladimir , Rodrigues Carlos H. M. , Ascher David B.
Published: January 2023

Biologics are one of the most rapidly expanding classes of therapeutics, but can be associated with a range of toxic properties. In small-molecule drug development, early identification of potential toxicity led to a significant reduction in clinical trial failures, however we currently lack robust qualitative rules or predictive tools for peptide- and protein-based biologics. To address this, we have manually curated the largest set of high-quality experimental data on peptide and protein toxicities, and developed CSM-Toxin, a novel in-silico protein toxicity classifier, which relies solely on the protein primary sequence. Our approach encodes the protein sequence information using a deep learning natural languages model to understand “biological” language, where residues are treated as words and protein sequences as sentences. The CSM-Toxin was able to accurately identify peptides and proteins with potential toxicity, achieving an MCC of up to 0.66 across both cross-validation and multiple non-redundant blind tests, outperforming other methods and highlighting the robust and generalisable performance of our model. We strongly believe the CSM-Toxin will serve as a valuable platform to minimise potential toxicity in the biologic development pipeline. Our method is freely available as an easy-to-use webserver.
Effects of Dimerization, Dendrimerization, and Chirality in p-BthTX-I Peptide Analogs on the Antibacterial Activity and Enzymatic Inhibition of the SARS-CoV-2 PLpro Protein
Authors: Bitencourt Natália Vitória , Righetto Gabriela Marinho , Camargo Ilana Lopes Baratella Cunha , de Godoy Mariana Ortiz , Guido Rafael Victorio Carvalho , Oliva Glaucius , Santos-Filho Norival Alves , Cilli Eduardo Maffud
Published: January 2023

Recent studies have shown that the peptide [des-Cys11,Lys12,Lys13-(p-BthTX-I)2K] (p-Bth) is a p-BthTX-I analog that shows enhanced antimicrobial activity, stability and hemolytic activity, and is easy to obtain compared to the wild-type sequence. This molecule also inhibits SARS-CoV-2 viral infection in Vero cells, acting on SARS-CoV-2 PLpro enzymatic activity. Thus, the present study aimed to assess the effects of structural modifications to p-Bth, such as dimerization, dendrimerization and chirality, on the antibacterial activity and inhibitory properties of PLpro. The results showed that the dimerization or dendrimerization of p-Bth was essential for antibacterial activity, as the monomeric structure led to a total loss of, or significant reduction in, bacterial activities. The dimers and tetramers obtained using branched lysine proved to be prominent compounds with antibacterial activity against Gram-positive and Gram-negative bacteria. In addition, hemolysis rates were below 10% at the corresponding concentrations. Conversely, the inhibitory activity of the PLpro of SARS-CoV-2 was similar in the monomeric, dimeric and tetrameric forms of p-Bth. Our findings indicate the importance of the dimerization and dendrimerization of this important class of antimicrobial peptides, which shows great potential for antimicrobial and antiviral drug-discovery campaigns.
Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis
Authors: Sagini Krizia , Urbanelli Lorena , Buratta Sandra , Emiliani Carla , Llorente Alicia
Published: January 2023

Altered cellular metabolism is a well-established hallmark of cancer. Although most studies have focused on the metabolism of glucose and glutamine, the upregulation of lipid metabolism is also frequent in cells undergoing oncogenic transformation. In fact, cancer cells need to meet the enhanced demand of plasma membrane synthesis and energy production to support their proliferation. Moreover, lipids are precursors of signaling molecules, termed lipid mediators, which play a role in shaping the tumor microenvironment. Recent methodological advances in lipid analysis have prompted studies aimed at investigating the whole lipid content of a sample (lipidome) to unravel the complexity of lipid changes in cancer patient biofluids. This review focuses on the application of mass spectrometry-based lipidomics for the discovery of cancer biomarkers. Here, we have summarized the main lipid alteration in cancer patients’ biofluids and uncovered their potential use for the early detection of the disease and treatment selection. We also discuss the advantages of using biofluid-derived extracellular vesicles as a platform for lipid biomarker discovery. These vesicles have a molecular signature that is a fingerprint of their originating cells. Hence, the analysis of their molecular cargo has emerged as a promising strategy for the identification of sensitive and specific biomarkers compared to the analysis of the unprocessed biofluid.
Sonoporation of the Round Window Membrane on a Sheep Model: A Safety Study
Authors: Kerneis Sandrine , Escoffre Jean-Michel , Galvin John J. , Bouakaz Ayache , Presset Antoine , Alix Corentin , Oujagir Edward , Lefèvre Antoine , Emond Patrick , Blasco Hélène , Bakhos David
Published: January 2023

Sonoporation using microbubble-assisted ultrasound increases the permeability of a biological barrier to therapeutic molecules. Application of this method to the round window membrane could improve the delivery of therapeutics to the inner ear. The aim of this study was to assess the safety of sonoporation of the round window membrane in a sheep model. To achieve this objective, we assessed auditory function and cochlear heating, and analysed the metabolomics profiles of perilymph collected after sonoporation, comparing them with those of the control ear in the same animal. Six normal-hearing ewes were studied, with one sonoporation ear and one control ear for each. A mastoidectomy was performed on both ears. On the sonoporation side, Vevo MicroMarker® microbubbles (MBs; VisualSonics—Fujifilm, Amsterdam, The Netherlands) at a concentration of 2 × 108 MB/mL were locally injected into the middle ear and exposed to 1.1 MHz sinusoidal ultrasonic waves at 0.3 MPa negative peak pressure with 40% duty cycle and 100 μs interpulse period for 1 min; this was repeated three times with 1 min between applications. The sonoporation protocol did not induce any hearing impairment or toxic overheating compared with the control condition. The metabolomic analysis did not reveal any significant metabolic difference between perilymph samples from the sonoporation and control ears. The results suggest that sonoporation of the round window membrane does not cause damage to the inner ear in a sheep model.
Development of an Oral Liquid Formulation of Nicardipine Hydrochloride Compounded with Simple Excipients for the Treatment of Pediatric Hypertension
Authors: Cavelier Marine , Gondé Henri , Costa Damien , Lamoureux Fabien , Pereira Tony , Buchbinder Nimrod , Varin Rémi , Hervouët Charles
Published: January 2023

Nicardipine hydrochloride is an anti-hypertensive drug that is used off-label to treat hypertension in children. A previous oral formulation of nicardipine hydrochloride was developed using a commercial vehicle as an excipient. However, ready-to-use vehicles are prone to supply shortages, and their composition may undergo substantial modifications. The aim of this study was to propose a new oral formulation of nicardipine hydrochloride 2 mg/mL using simple excipients. The formulation included hydroxypropylmethylcellulose, simple syrup, polysorbate 80, sodium saccharin, citrate buffer, strawberry flavor and 0.2% potassium sorbate. The uniformity of content was maintained before and after agitation. Nicardipine hydrochloride concentration assessed by HPLC-MS/MS remained above 90% for 365 days before opening and for 28 days after opening. pH and osmolality were maintained throughout the study, and no microbial contamination was observed. The uniformity of mass of the delivered doses was evaluated using four different devices. A new oral formulation of nicardipine hydrochloride 2 mg/mL was developed using simple and safe excipients. Pharmacological and clinical parameters remain to be assessed and compared with those of the previous formulation.
Peptide Vaccines in Melanoma: Chemical Approaches towards Improved Immunotherapeutic Efficacy
Authors: Biri-Kovács Beáta , Bánóczi Zoltán , Tummalapally Anitha , Szabó Ildikó
Published: January 2023

Cancer of the skin is by far the most common of all cancers. Although the incidence of melanoma is relatively low among skin cancers, it can account for a high number of skin cancer deaths. Since the start of deeper insight into the mechanisms of melanoma tumorigenesis and their strong interaction with the immune system, the development of new therapeutical strategies has been continuously rising. The high number of melanoma cell mutations provides a diverse set of antigens that the immune system can recognize and use to distinguish tumor cells from normal cells. Peptide-based synthetic anti-tumor vaccines are based on tumor antigens that elicit an immune response due to antigen-presenting cells (APCs). Although targeting APCs with peptide antigens is the most important assumption for vaccine development, peptide antigens alone are poorly immunogenic. The immunogenicity of peptide antigens can be improved not only by synthetic modifications but also by the assistance of adjuvants and/or delivery systems. The current review summarizes the different chemical approaches for the development of effective peptide-based vaccines for the immunotherapeutic treatment of advanced melanoma.
The Impact of Radio Frequency Waves on the Plasma Density in the Tokamak Edge
Authors: Van Eester Dirk , Tournay Nil
Published: January 2023

A simple model is presented to describe how the radio frequency electromagnetic field modifies the plasma density the antenna faces in tokamaks. Aside from “off-the-shelf” equations standardly used to describe wave-plasma interaction relying on the quasilinear approach, it invokes the ponderomotive force in presence of the confining static magnetic field. The focus is on dynamics perpendicular to the Bo magnetic field. Stronger fields result in density being pushed further away from the launcher and in stronger density asymmetry along the antenna.
Modeling the Magnetic Field of the Inner Corona in a Radially Expanding Solar Wind
Authors: Tlatov Andrey G. , Berezin Ivan
Published: January 2023

The magnetic field in the interplanetary medium is formed by the action of magnetic field sources on the photosphere of the Sun and currents in the expanding atmosphere of the Sun and the solar wind. In turn, the high-speed plasma flow changes the configuration of the magnetic field lines. The problem of determining the parameters of the magnetic field near the Sun is thus a three-dimensional problem of the interaction of the magnetic field and the plasma of the solar wind. We present analytical expressions for calculating the total magnetic field vector B→(r, θ, ϕ) (in spherical coordinates) for a radially expanding solar wind flow of finite conductivity. The parameters of the solar wind are given in the form of a dimensionless magnetic Reynolds number given as an arbitrary function of the radius, r: Rm = rσμv=ξ(r), where σ, μ, and v denote, respectively, the conductivity, magnetic permeability, and velocity of the solar wind. The solution for the magnetic field components is obtained in the form of a decomposition in spherical functions and a radial part depending on the distance from the Sun. Examples of calculations of the configuration of magnetic fields and structures of the solar corona for the solar eclipse of 21 August 2017 are given.
Analysis of Fast Fluorescence Kinetics of a Single Cyanobacterium Trapped in an Optical Microcavity
Authors: Rammler Tim , Wackenhut Frank , Rapp Johanna , zur Oven-Krockhaus Sven , Forchhammer Karl , Meixner Alfred J. , Harter Klaus
Published: January 2023

Photosynthesis is one the most important biological processes on earth, producing life-giving oxygen, and is the basis for a large variety of plant products. Measurable properties of photosynthesis provide information about its biophysical state, and in turn, the physiological conditions of a photoautotrophic organism. For instance, the chlorophyll fluorescence intensity of an intact photosystem is not constant as in the case of a single fluorescent dye in solution but shows temporal changes related to the quantum yield of the photosystem. Commercial photosystem analyzers already use the fluorescence kinetics characteristics of photosystems to infer the viability of organisms under investigation. Here, we provide a novel approach based on an optical Fabry–Pérot microcavity that enables the readout of photosynthetic properties and activity for an individual cyanobacterium. This approach offers a completely new dimension of information, which would normally be lost due to averaging in ensemble measurements obtained from a large population of bacteria.
Molecular Characterization and Efficacy Evaluation of Transgenic Maize Harboring cry2Ab-vip3A-cp4epsps for Insect Resistance and Herbicide Tolerance
Authors: Liu Fantao , Liu Yuan , Zou Junjie , Zhang Lan , Zheng Hongyan , Luo Yanzhong , Wang Xiaoping , Wang Lei
Published: January 2023

Insect infestation and weed interference have a seriously negative impact on the growth, yield, and grain quality of maize. In this study, transgenic maize plants harboring three exogenous genes, cry2Ab, vip3A, and cp4epsps, that were constructed into a single T-DNA were developed for protection against insects and weeds. The transgene integration sites on the chromosomes in two transgenic maize events, CVC-1 and CVC-2, were determined using whole genome sequencing and specific PCR detection. As revealed by laboratory insect bioassays, these two transgenic events exhibited strong insecticidal toxicity against three major species of Lepidoptera insects, including Mythimna separata, Helicoverpa armigera, and Spodoptera frugiperda, with mortality rates exceeding 96%, 100%, and 100%, respectively, after six days of infestation. In addition, CVC-1 exhibited a high tolerance to glyphosate under field conditions. The successful expressions of cry2Ab, vip3A, and cp4epsps in various tissues at different developmental stages of CVC-1 were validated at the transcriptional and translational levels using quantitative real-time reverse transcription PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. These findings demonstrated that the transgenic maize CVC-1 developed using this triple gene construct has excellent insect resistance and herbicide tolerance, which may provide a valuable germplasm resource and data support for future maize breeding of insect and weed control.
Custom-Developed Reflection–Transmission Integrated Vision System for Rapid Detection of Huanglongbing Based on the Features of Blotchy Mottled Texture and Starch Accumulation in Leaves
Authors: Xu Qian , Cai Jianrong , Ma Lixin , Tan Bin , Li Ziqi , Sun Li
Published: January 2023

Huanglongbing (HLB) is a highly contagious and devastating citrus disease that causes huge economic losses to the citrus industry. Because it cannot be cured, timely detection of the HLB infection status of plants and removal of diseased trees are effective ways to reduce losses. However, complex HLB symptoms, such as single HLB-symptomatic or zinc deficiency + HLB-positive, cannot be identified by a single reflection imaging method at present. In this study, a vision system with an integrated reflection–transmission image acquisition module, human–computer interaction module, and power supply module was developed for rapid HLB detection in the field. In reflection imaging mode, 660 nm polarized light was used as the illumination source to enhance the contrast of the HLB symptoms in the images based on the differences in the absorption of narrow-band light by the components within the leaves. In transmission imaging mode, polarization images were obtained in four directions, and the polarization angle images were calculated using the Stokes vector to detect the optical activity of starch. A step-by-step classification model with four steps was used for the identification of six classes of samples (healthy, HLB-symptomatic, zinc deficiency, zinc deficiency + HLB-positive, magnesium deficiency, and boron deficiency). The results showed that the model had an accuracy of 96.92% for the full category of samples and 98.08% for the identification of multiple types of HLB (HLB-symptomatic and zinc deficiency + HLB-positive). In addition, the classification model had good recognition of zinc deficiency and zinc deficiency + HLB-positive samples, at 92.86%.
In Vitro Comparison of Surface Roughness, Flexural, and Microtensile Strength of Various Glass-Ionomer-Based Materials and a New Alkasite Restorative Material
Authors: Kaptan Alper , Oznurhan Fatih , Candan Merve
Published: January 2023

This study aims to evaluate the physical properties of Cention N and various glass-ionomer-based materials in vitro. The groups were obtained as follows: Group 1 (LC-Cent): light-cured Cention N; Group 2 (SC-Cent): self-cured Cention N; Group 3 (COMP): composite (3M Universal Restorative 200); Group 4 (DYRA): compomer (Dyract XP); Group 5 (LINER): Glass Liner; Group 6 (FUJI): FujiII LC Capsule; and Group 7 (NOVA): Nova Glass LC. For the microtensile bond strength (μTBS) test, 21 extracted human molar teeth were used. The enamel of the teeth was removed, and flat dentin surfaces were obtained. Materials were applied up to 3 mm, and sticks were obtained from the teeth. Additionally, specimens were prepared, and their flexural strength and surface roughness (Ra) were evaluated. Herein, data were recorded using SPSS 22.0, and the flexural strength, μTBS, and Ra were statistically analyzed. According to the surface roughness tests, the highest Ra values were observed in Group 6 (FUJI) (0.33 ± 0.1), whereas the lowest Ra values were observed in Group 2 (SC-Cent) (0.17 ± 0.04) (p < 0.05). The flexural strengths of the materials were compared, and the highest value was obtained in Group 2 (SC-Cent) (86.32 ± 15.37), whereas the lowest value was obtained in Group 5 (LINER) (41.75 ± 10.05) (p < 0.05). When the μTBS of materials to teeth was evaluated, the highest μTBS was observed in Group 3 (COMP) (16.50 ± 7.73) and Group 4 (DYRA) (16.36 ± 4.64), whereas the lowest μTBS was found in Group 7 (NOVA) (9.88 ± 1.87) (p < 0.05). According to the μTBS results of materials-to-materials bonding, both Group 2 (SC-Cent) and Group 1 (LC-Cent) made the best bonding with Group 3 (COMP) (p < 0.05). It can be concluded that self-cured Cention N had the highest flexural strength and lowest surface roughness of the seven materials tested. Although the bond strength was statistically lower than conventional composites and compomers, it was similar to resin-modified glass ionomer cements. Additionally, the best material-to-material bonding was found between self-cured Cention N and conventional composites.
Poly(lactic acid) and Nanocrystalline Cellulose Methacrylated Particles for Preparation of Cryogelated and 3D-Printed Scaffolds for Tissue Engineering
Authors: Leonovich Mariia , Korzhikov-Vlakh Viktor , Lavrentieva Antonina , Pepelanova Iliyana , Korzhikova-Vlakh Evgenia , Tennikova Tatiana
Published: January 2023

Different parts of bones possess different properties, such as the capacity for remodeling cell content, porosity, and protein composition. For various traumatic or surgical tissue defects, the application of tissue-engineered constructs seems to be a promising strategy. Despite significant research efforts, such constructs are still rarely available in the clinic. One of the reasons is the lack of resorbable materials, whose properties can be adjusted according to the intended tissue or tissue contacts. Here, we present our first results on the development of a toolbox, by which the scaffolds with easily tunable mechanical and biological properties could be prepared. Biodegradable poly(lactic acid) and nanocrystalline cellulose methacrylated particles were obtained, characterized, and used for preparation of three-dimensional scaffolds via cryogelation and 3D printing approaches. The composition of particles-based ink for 3D printing was optimized in order to allow formation of stable materials. Both the modified-particle cytotoxicity and the matrix-supported cell adhesion were evaluated and visualized in order to confirm the perspectives of materials application.
Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection
Authors: Jiang Yuhui , Tang Xiaoxuan , Li Tao , Ling Jue , Ge Yifan , Yang Yumin
Published: January 2023

The liver is the most complex vascular anatomy of all human organs, with extremely rich blood flow and fragile texture. Massive liver bleeding usually occurs after traumatic liver injury, causing severe systematic issues. Thus, bleeding control is critical in hindering mortality rates and complications in patients. In this study, non-compression hemostasis materials based on chitosan lactate particles (CLP) were developed for handling liver bleeding after injuries. CLP showed good blood biocompatibility and antibacterial performance against S. aureus. Taking advantage of the vital capacity of CLP to promote red blood cell and platelet adhesion, CLP exhibited in vivo homeostasis properties as non-compression hemostasis materials for traumatic liver injury, both in SD rats, New Zealand rabbits, or in beagles. Whereas CLP has better hemostasis than the commercial hemostatic agent Celox™.
Palladium Nanoparticle-Loaded Mesostructural Natural Woods for Efficient Water Treatment
Authors: Wang Zirun , Jia Chao , Xiang Hengxue , Zhu Meifang
Published: January 2023

Natural wood with oriented microchannels and unique multi-level structures is an ideal candidate for making water treatment membranes. Here, palladium nanoparticles are loaded into different kinds of natural woods and the degradation property of the wood membranes for organic pollutants are investigated. The water flux of hardwoods is significantly higher than that of softwood due to the existence of large vessel elements. For the single pollutant, both hardwood and softwood show high degradation efficiency for methylene blue and methylene orange, while the degradation efficiency of the softwoods for 4-nitrophenol is significantly higher than that of the hardwoods due to their lower water flux. For the mixed pollutants, all the wood membranes have a good degradation property for different concentrations of methylene blue in polluted water, while the degradation efficiency of high concentration methylene orange and 4-nitrophenol is low. Our work will provide some guidance for the degradation of organic pollutants in actual polluted water.
Regulation of Intersubunit Interactions in Homotetramer of Glyceraldehyde-3-Phosphate Dehydrogenases upon Its Immobilization in Protein—Kappa-Carrageenan Gels
Authors: Makshakova Olga , Antonova Maria , Bogdanova Liliya , Faizullin Dzhigangir , Zuev Yuriy
Published: January 2023

Polysaccharides, being biocompatible and biodegradable polymers, are highly attractive as materials for protein delivery systems. However, protein–polysaccharide interactions may lead to protein structural transformation. In the current study, we analyze the structural adjustment of a homotetrameric protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), upon its interactions with both flexible coil chain and the rigid helix of κ-carrageenan. FTIR spectroscopy was used to probe the secondary structures of both protein and polysaccharide. Electrostatically driven protein–polysaccharide interactions in dilute solutions resulted in an insoluble complex formation with a constant κ-carrageenan/GAPDH ratio of 0.2, which amounts to 75 disaccharide units per mole of protein tetramer. Upon interactions with both coiled and helical polysaccharides, a weakening of the intersubunit interactions was revealed and attributed to a partial GAPDH tetramer dissociation. In turn, protein distorted the helical conformation of κ-carrageenan when co-gelled. Molecular modeling showed the energy favorable interactions between κ-carrageenan and GAPDH at different levels of oligomerization. κ-Carrageenan binds in the region of the NAD-binding groove and the S-loop in OR contact, which may stabilize the OP dimers. The obtained results highlight the mutual conformational adjustment of oligomeric GAPDH and κ-carrageenan upon interaction and the stabilization of GAPDH’s dissociated forms upon immobilization in polysaccharide gels.
Removal of Sulfide Ions from Kraft Washing Effluents by Photocatalysis with N and Fe Codoped Carbon Dots
Authors: Luo Hao , Liu Hao , Sun Chengwu
Published: January 2023

N and Fe codoped carbon dots (N,Fe-CDs) were fabricated from citric acid, L-glutamic acid and ferric chloride via a hydrothermal method for the photocatalytic removal of S2− from kraft washing effluents (KWE). The N,Fe-CDs were fluorescent nanoparticles (average size of 3.18 nm) and catalyzed the oxidation of S2− following a first-order kinetic model with an activation energy of 33.77 kJ/mol. The N,Fe-CDs tolerated elevated temperatures as high as 80 °C without catalyst deactivation. The N,Fe-CDs catalysts were reusable for at least four cycles, preserving over 90% of the activity. In the treatment of KWE from the kraft pulping of eucalyptus, the concentration of S2− was decreased by the N,Fe-CDs from 1.19 to 0.41 mmol/L in 6 h. Consequently, near complete remediation was obtained in 24 h. In addition, half of the chemical oxygen demand was removed after treatment with 500 mg/L of the N,Fe-CDs. In addition, the present photocatalyst was safe within a concentration of 200 mg/L, as indicated by the acetylcholinesterase inhibition test. Our findings may help develop a cleaner production process for kraft brownstock washing.
Influence of the Temperature-Dependent Characteristics of CFRP Mechanical Properties on the Critical Axial Force of Drilling Delamination
Authors: Wang Hongxiao , Wu Yahang , Zhang Yi , Zhang Xiaohui
Published: January 2023

Previous studies have often assumed that the mechanical properties of Carbon Fibre Reinforced Plastics (CFRP) remain unchanged during drilling. In fact, due to the increase in drilling temperature, the mechanical properties of the composites change greatly, and this then affects the critical force. In addition, previous studies have often assumed that the failure mode of CFRP drilling was a type I crack failure. In fact, due to the complexity of the CFRP drilling process, the failure modes are often coupled with different failure modes, so type I cracks alone cannot reflect the actual cracking situation. Therefore, a three-dimensional drilling Finite Element Modeling (FEM) was established to analyze the failure modes of CFRP drilling delamination, and the I/III mode was determined; then, a new drilling critical force mechanics model, which considers the temperature dependence of CFRP mechanical properties and the failure modes of CFRP drilling delamination, was established based on the classical drilling critical force mechanics model; the results of the mechanics model were validated by drilling critical force experiments under different temperatures. The effects of the temperature dependence of CFRP mechanical properties on the drilling critical force were investigated and analyzed.
Photocrosslinked Fish Collagen Peptide/Chitin Nanofiber Composite Hydrogels from Marine Resources: Preparation, Mechanical Properties, and an In Vitro Study
Authors: Yano Shinya , Yamaguchi Kei , Shibata Mitsuhiro , Ifuku Shinsuke , Teramoto Naozumi
Published: January 2023

Fish collagen peptide (FCP) is a water-soluble polymer with easy accessibility, bioactivity, and reactivity due to its solubility. The gelation of FCP can be carried out by chemical crosslinking, but the mechanical strength of FCP hydrogel is very low because of its intrinsically low molecular weight. Therefore, the mechanical properties of FCP gel should be improved for its wider application as a biomaterial. In this study, we investigated the mechanical properties of M-FCP gel in the context of understanding the influence of chitin nanofibers (CHNFs) on FCP hydrogels. FCP with a number average molecular weight (Mn) of ca. 5000 was reacted with glycidyl methacrylate (GMA) and used for the preparation of photocrosslinked hydrogels. Subsequently, composite hydrogels of methacrylate-modified FCP (M-FCP) and CHNF were prepared by the photoirradiation of a solution of M-FCP containing dispersed CHNF at an intensity of ~60 mW/cm2 for 450 s in the presence of 2-hydroxy-1-[4-(hydroxyethoxy)phenyl]-2-methyl-1-propanone (Irgacure 2959) as a photoinitiator. Compression and tensile tests of the FCP hydrogels were carried out using a universal tester. The compression and tensile strength of the hydrogel increased 10-fold and 4-fold, respectively, by the addition of 0.6% CHNF (20% M-FCP), and Young’s modulus increased 2.5-fold (20% M-FCP). The highest compression strength of the M-FCP/CHNF hydrogel was ~300 kPa. Cell proliferation tests using fibroblast cells revealed that the hydrogel with CHNF showed good cell compatibility. The cells showed good adhesion on the M-FCP gel with CHNF, and the growth of fibroblast cells after 7 days was higher on the M-FCP/CHNF gel than on the M-FCP gel without CHNF. In conclusion, we found that CHNF improved the mechanical properties as well as the fibroblast cell compatibility, indicating that M-FCP hydrogels reinforced with CHNF are useful as scaffolds and wound-dressing materials.
The Influence of Azidated Glycerol as a New Low Temperature Stabilizer on the Colloidal Properties of Natural Rubber Latex: For Latex Marine Transportation
Authors: Siti Nuraya A. S. , Azniwati A. A. , Baharin A. , Azura A. R. , Yhaya M. F.
Published: January 2023

Natural rubber latex (NRL) is normally transported to a destination in colloid/liquid form. It requires large storage containers such as drums and the probability of latex leakage during transportation is high. This is prevalent especially when transporting latex by sea. To prevent latex spillage, the liquid form of NRL is transformed into solid/frozen latex by freezing. However, the coagulation/destabilization of NRL by freezing has been acknowledged as a problem for years. Therefore, this study proposed a new low temperature stabilizer named azidated glycerol (AG) to be incorporated in NRL liquid before the freezing process. AG was prepared by a chemical reaction of pure glycerol with sodium azide. NRL containing AG was then frozen at a temperature of −4 °C. After 24 h of freezing, the frozen latex was thawed at ambient temperature for 1 h followed by heating in a water bath at 40 °C for another 1 h. The regenerated latex was then allowed to stand at room temperature before testing. The effect of AG on the colloid properties before and after the freeze–thaw processes was studied. The production of AG was confirmed by the appearance of a peak in the range of 2160–2120 cm−1, corresponding to N=N=N stretching, confirming the introduction of an azide group into the glycerol molecule. Modifying NRL with AG did not significantly influence the TSC of latex. Increasing the AG content up to 0.4 phr resulted in an increase in MST from 699 s to 828 s. An AG content of 0.2 phr resulted in the highest anionically stabilized latex as indicated by zeta potential values of −59.63 mV (before freezing) and −56.27 mV (after thawing). It is concluded that the AG produced in this study can be used as an anti-freeze stabilizer for NRL and is suitable for latex marine transportation.
“It Makes a Difference!” Religion and Self-Assessed Health among Healthcare Support Professionals of Asian-Indian Origin
Authors: Roy Moushumi
Published: January 2023

Objective: Mixed evidence exists in the research examining the effects of religion on health outcomes. Due to the severity of health conditions experienced by immigrants and racial and ethnic minorities in the US during the COVID-19 pandemic, this research aims to examine the influence of religious involvement on self-assessed health among healthcare support professionals of Asian Indian origin living in the US. Method: The study used oral history narratives of phenomenological tradition, obtained through interviews of 16 healthcare support professionals of Asian-Indian origin and from three different religious backgrounds. The study cohort was made up of individuals residing in the greater Detroit and Lansing areas in Michigan. Findings: Although the narratives indicated that religious engagement plays a positive role in health outcomes among care professionals, simultaneously, it was found that religion-specific meaning intersects their immigration, settlement, family expectations, and work-related experiences in the US. Future research should extend the scholarship examining gender differences in the effect of religion on self-assessed health among healthcare support professionals of Asian-Indian origin.
Media Representations of Gendered Minority Practices: The Case of Polygamy in Israel
Authors: Kiwanuka Sophia Abela , Parker Asia , Ben Shitrit Lihi
Published: January 2023

This paper critically examines how the mainstream media in Israel frame the phenomenon of polygamy among the minority Palestinian Bedouin community within the country. We identify four prominent media frames: (1) an “orientalist” frame, which considers Muslim women as in need of saving from their own culture and religion’s oppression by a modernizing state; (2) a “securitization” frame, which links the practice of polygamy to threats to the state’s security and to “Islamic terrorism;” (3) an “existential threat” frame, which reflects the Israeli Jewish majority’s anxieties about a demographic battle between Jews and Muslims in the country; and finally, (4) a “women’s rights” frame, which is the least prevalent, that addresses polygamy from the perspective of women’s equality and equal citizenship, and which is critical of the discriminatory policies of the state. Theoretically, the paper explicates how the media utilizes minority gendered practices to amplify Islamophobic sentiments in relation to a Muslim community, and how alternative framing and the featuring of critical Muslim women’s voices in the media might mitigate such harmful effects.
The Use and Misuse of Zakāh Funds by Religious Institutions in North America
Authors: Wahb Yousef Aly
Published: January 2023

Despite being a foundational practice in Islam, deeply rooted in law and reflected in the theological and spiritual concepts of wealth and sustenance (rizq), discussions of applying obligatory alms (zakāh) rulings to majority non-Muslim countries are limited. The Muslim’s spiritual attitude toward finances is informed by a theological view that all forms of wealth ultimately belong to God. Sunni Muslim theologians define rizq to be what one actually (not potentially) consumes and benefits from (not possesses), which, alongside plentiful verses and Prophetic traditions, continuously motivate philanthropic giving without fearing scarcity. This article aims to investigate some major issues resulting from the unregulated procedures of zakāh collection and disbursement as practiced by North American Muslim organizations and religious leaders. The article (1) doctrinally analyzes how North American practices diverge from the rules of Islamic law (fiqh) regarding zakāh distribution, (2) examines the ramifications of contemporary Eastern–Western legal opinions (fatāwā) expanding the eligibility of charitable institutions to receive zakāh, and (3) investigates the practices of administering zakāh resources.
The Narrative Foundations of Radical and Deradicalizing Online Discursive Spaces: A Comparison of the Cases of Generation Islam and Jamal al-Khatib in Germany
Authors: Ali Rami , Özvatan Özgür , Walter Linda
Published: January 2023

Radical/extremist Islamist actors use social media to disseminate uncompromising stories of monist religious political orders and identities. As a reaction, counter-movements to online Islamist radicalism/extremism emerged in Western societies (and beyond), while uncertainty about effective outcomes remains widespread. In a bid to understand how inclusionary and exclusionary discursive spaces are created, we ask: How do some Muslim actors create discursive spaces open to self-reflection, pluralism and liberal-democratic principles, while others construct illiberal, particularistic and non/anti-democratic spaces? To respond to this question, we compare two contrasting storytellers, one who agitates for exclusionary Islamist radicalism/extremism (Generation Islam) and one who offers inclusionary prevention and deradicalization work against that (Jamal al-Khatib). We draw on novel narrative approaches to the Discourse Historical Approach (DHA) in Critical Discourse Studies (CDS), via which we compare text-level and context-level narratives disseminated about three Muslim-related crises: the racist terrorist attacks/genocide to represent the national, European and global level. Our two-layered, DHA-inspired narrative analysis illustrates that, at the level of text, narrative persuasion varies between both contrasting actors. While Jamal al-Khatib disseminates persuasive stories, Generation Islam is much less invested in narrative persuasion; it seems to address an already convinced audience. These two text-level strategies reveal their meaning in two antagonistic narrative genres: Jamal al-Khatib’s “self-reflexive savior” creates an inclusionary discursive space represented in a self-ironic narrative genre, while Generation Islam’s ”crusading savior” manufactures an exclusionary discursive space represented in a romance featuring a nostalgic return to the particularistic Islamic umma.
Atonement, Returning, and Repentance in Islam
Authors: Khalil Atif
Published: January 2023

The aim of this article is to demonstrate how in Islam the principle mechanism for atonement lies in tawba (returning, repentance). Divided into four sections, and drawing primarily on the literature of classical Sufism, the analysis begins by defining some key terms related to the idea of atonement, with special attention to the language of the Quran. Then it outlines three conditions of returning, repentance, and atonement, delineated by classical Muslim authorities, before turning to a brief overview of the concept of amending wrongs or settings matters aright. It concludes with some final remarks about the possibilities of atonement available until death, and the soteriological role divine mercy is believed to play in the posthumous states of the soul.
An Anthropological Outline of the Sutta Nipāta: The Contemplative Experience in Early Buddhist Poetry
Authors: Divino Federico
Published: January 2023

By examining the Sutta Nipāta, considered one of the collections containing the oldest texts of the Pāli canon, this study aims to reconstruct social, historical, and anthropological aspects of archaic Buddhist doctrine at the time of its definition in dialectic with the forces of orthodoxy and constituted power in 6th century BCE India. This study also provides important insights into the development and definition of contemplative practices devoted to the search for the ‘absolute’ (paramattha and brahmavihāra), which has often received little attention in meditation studies.
Czesław Miłosz’s Translations as “Re-Visioning” of the Psalms: Poetry and Eschatology
Authors: Chrusciel Ewa
Published: January 2023

This article focuses on Czesław Miłosz’s translations of parts of the Psalms and their influence on his poetry. For Miłosz, poetry had an eschatological dimension, a view deeply influenced by his distant cousin, the Lithuanian poet and playwright Oscar Miłosz. In his essay “A Few Words on Poetry,” Oscar Miłosz claimed that since prehistoric times, poetry has always followed the mysterious movements of the great soul of the people. He criticized his contemporaries—the French Symbolists—for their elitism, which perpetuated the schism between the poet and the great human family. He predicted that the new poetry would be that of the Bible: “a spacious prose hammered into verses.” For him, a truly inspired poet of the future will be able to transcend his paltry ego. Czesław Miłosz—thanks to this significant influence—resisted literary fashions. Moreover, in times of despair or dry spells in his writing, Miłosz would turn to translating the Bible. In his poem “Ars Poetica?” he writes: “I have always aspired to a more spacious form / that would be free from the claims of poetry or prose/and would let us understand each other without exposing / the author or reader to sublime agonies.”
Amplifying the Dalit Pentecostal Historical Narrative amid the Persistent Syrian Christian ‘Privileged’ Narrative in Kerala
Authors: Varghese Meloottu Allan
Published: January 2023

While studies indicate that Pentecostal teachings center the egalitarian move of the Holy Spirit with empowering effects on the social lives of the Dalits in the South Indian Pentecostal landscape, the persistence of ethnocentric behavior from “Syrian Christian” Pentecostals continues. Hence, this paper focuses on exploring the historical development of a Syrian Christian identity marker as a privileged one that became prefixed by the Pentecostals in Kerala, especially in Travancore. In this regard, this paper seeks to answer questions such as: How did caste dynamics structure the formation of Christian communities in Kerala? How did the historical Western missionary interactions deal with local caste-influenced distinctions (especially between the “Syrian Christian” and Dalit communities)? How did Pentecostalism challenge and perpetuate the Syrian Christian caste identity? Through an intersectional (religion and caste) historical analysis, this paper shows that the Syrian Christian community has been integrated into the caste system for the last two thousand years of Christian history in Kerala. At the same time, via a more inclusive historical narrative, the paper highlights Dalit conversions to Christianity since the sixteenth century as a critique against the privileged status of Syrian Christianity, even more so when Pentecostalism arrived at its shores, impacting the emergence of Dalit Pentecostalism.
Modern Shia Islamic and Jewish Political Theosophy: An Elective Affinity?
Authors: Tzfadya Ezra
Published: January 2023

The present study will focus on core parallels and nodes of theopolitical exchange between the two most politically and theologically consequential jurist “theosophers” of the twentieth century, the Religious Zionist founding father, the Jewish Rav Avraham Yitzchak Hacohen Kook (1865–1935), and the Shia Islamic Ayatollah Ruhollah Khomeini (1900–1989). Unquestioned masters of the tradition of both medieval philosophy and mysticism, as well as the theosophies of the early-modern and modern eras, both Kook and Khomeini attempted to embed the rhetoric of theosophy within revolutionary notions of both clerical religious authority and the necessity of their respective nomoi to assume political form. The study will also correlate contemporary Shia reformist theosophies undergirded with anti-theocratic exoteric postures with pre-WW2 German-Jewish “existence philosophies” as represented by Franz Rosenzweig, noting a common appreciation for what the study will term “theopolitical risk”. It argues that the retrieval of medieval Judeo-Islamic political philosophy for the successful negotiation of reason and revelation in modernity against both theocratic juridical extremism and the iron cages of positivistic-realist secularism must be rethought in light of the theopolitics coursing through Iran and Israel, two states at the geographic periphery though fully within the horizons of the Modern West.