bluuebunny/crossref_metadata_2025 huggingface dataset:
Low Baseline Sympathetic Tone Correlates to a Greater Blood Pressure Change in the Cold Pressor Test
Authors: Youssef Marylen , Ghassemi Azadeh , Carvajal Gonczi Catalina Marysol , Kugathasan Thiffya Arabi , Kilgour Robert D. , Darlington Peter J.
Published: May 2018

BACKGROUND: The cold pressor test (CPT) involves acute hand or foot exposure to cold water. CPT hyper-responders have unique traits, including risk of hypertension and a greater vasoconstrictor reserve and g force tolerance compared to hypo-responders. The purpose of this study
was to uncover differences in cardiovascular and sympathetic biomarkers between responder types.METHODS: Healthy volunteers (N = 30) submerged one hand into cold water (3.3 ± 0.8°C) for 5 min. Blood pressure, heart rate, cardiac output, and cardiac parameters were
recorded using an automated monitor, impedance cardiography, and a beat-to-beat monitoring system. We analyzed for salivary α-amylase (SαA), which is a convenient biomarker of the sympathetic nervous system. Subjects were stratified post hoc into hyper-responders (≥ 22 mmHg)
and hypo-responders (< 22 mmHg) based on change in systolic blood pressure during CPT.RESULTS: Hyper-responders had a significantly lower baseline heart rate (64 ± 7 bpm), cardiac output (5.6 ± 0.9 L · min−1), and SαA (60 ± 37
U · mL−1) compared to hypo-responders (73 ± 9 bpm, 6.9 ± 1.3 L · min−1, 165 ± 122 U · mL−1). During the cold immersion, hyper-responders had significantly higher systolic blood pressure (150 ±
14 mmHg), diastolic blood pressure (91 ± 10 mmHg), mean arterial pressure (129 ± 17 mmHg), and systemic vascular resistance (1780 ± 640 dyn · s−1 · cm−5) than hypo-responders (130 ± 14 mmHg, 81 ± 10 mmHg, 110
± 9 mmHg, 1290 ± 220 dyn · s−1 · cm−5). The change in systolic blood pressure correlated with baseline SαA (r = −0.455, P = 0.011) and baseline heart rate (r = −0.374, P = 0.042).DISCUSSION:
Baseline characteristics influenced by sympathetic tone such as SαA, heart rate, and cardiac output are indicative of responses to CPT. Our data supports the use of baseline values to predict blood pressure response to acute cold exposure and indicates an intrinsic difference between
CPT responder phenotypes.Youssef M, Ghassemi A, Carvajal Gonczi CM, Kugathasan TA, Kilgour RD, Darlington PJ. Low baseline sympathetic tone correlates to a greater blood pressure change in the cold pressor test. Aerosp Med Hum Perform. 2018; 89(6):503–509.
Individual Differences in Adaptability to Isolated, Confined, and Extreme Environments
Authors: Bartone Paul T. , Krueger Gerald P. , Bartone Jocelyn V.
Published: May 2018

INTRODUCTION: Future deep space missions will expose astronauts to more intense stressors than previously encountered. Isolation will be greater and more prolonged, living and work areas more confined, and communications and resupply channels to Earth longer and less reliable.
Astronauts will need to function more autonomously, with less guidance and support from Earth. Thus, it is important to select and train astronauts who can adapt and function effectively under extreme and variable conditions. In order to identify factors linked to individual adaptability,
we conducted a systematic review of the literature on cognitive and behavioral adaptation to isolated, confined, and extreme (ICE) environments.METHODS: We searched PubMed, Embase, Web of Science, and PsychINFO databases for studies addressing individual adaptability to ICE environments.
Studies were rated for quality and fidelity to long-duration space missions and key results extracted.RESULTS: There were 73 studies that met all inclusion criteria. Adaptability attributes for ICE environments include intelligence, emotional stability, self-control, openness, achievement
facets of conscientiousness, optimism, mastery, introversion, hardiness, task-oriented coping, past experience, low need for social support, and adequate sleep.DISCUSSION: This review identifies individual factors linked to adaptability under ICE conditions. Further studies
are needed to verify causal directions and determine the relative importance of these factors.Bartone PT, Krueger GP, Bartone JV. Individual differences in adaptability to isolated, confined, and extreme environments. Aerosp Med Hum Perform. 2018; 89(6):536–546.
The Sound of Words Evokes Affective Brain Responses
Authors: Aryani Arash , Hsu Chun-Ting , Jacobs Arthur M.
Published: May 2018

The long history of poetry and the arts, as well as recent empirical results suggest that the way a word sounds (e.g., soft vs. harsh) can convey affective information related to emotional responses (e.g., pleasantness vs. harshness). However, the neural correlates of the affective potential of the sound of words remain unknown. In an fMRI study involving passive listening, we focused on the affective dimension of arousal and presented words organized in two discrete groups of sublexical (i.e., sound) arousal (high vs. low), while controlling for lexical (i.e., semantic) arousal. Words sounding high arousing, compared to their low arousing counterparts, resulted in an enhanced BOLD signal in bilateral posterior insula, the right auditory and premotor cortex, and the right supramarginal gyrus. This finding provides first evidence on the neural correlates of affectivity in the sound of words. Given the similarity of this neural network to that of nonverbal emotional expressions and affective prosody, our results support a unifying view that suggests a core neural network underlying any type of affective sound processing.
In Vivo Level of Poly(ADP-ribose)
Authors: Miwa Masanao , Ida Chieri , Yamashita Sachiko , Kouyama Kenichi , Kuroda Yasuhito , Eguchi Takayuki , Ohta Narumi , Sato Teruaki , Tsuda Masataka , Tanaka Masakazu
Published: May 2018

PolyADP-ribosylation is a post-translational modification that plays key roles in cellular physiological functions and DNA damage responses. PolyADP-ribosylation is finely and dynamically regulated by various enzymes and factors involved in the synthesis and degradation of poly(ADP-ribose) (PAR). To better understand the function of polyADP-ribosylation, it is necessary to quantify and monitor the change of the in vivo level of PAR, the product of polyADP-ribosylation, which is rapidly turning over and kept in quite low level in cells or in organs. Recent developments of potent inhibitors of polyADP-ribosylation is expected to kill BRCA1/2-mutated breast cancer cells and ovarian cancer cells (synthetic lethality). To know the efficacy of these inhibitors in vivo, it is necessary to develop highly sensitive and reproducible methods to know PAR levels within cells or organs. However there have been several difficulties in measuring the physiologically low level of PAR without artefacts. Our experiments recently clarified that the method of sample preparation is very important in addition to the sensitivity and specificity. From reviewing the literature, including ours, we would like to emphasize the importance of the procedures of sample preparation for the assay, in addition to the sensitivity by comparing the reported PAR levels in vivo.
Influences of Cr and Co on the Growth of Thermally Grown Oxide in Thermal Barrier Coating during High-Temperature Exposure
Authors: Zhang Weifang , Zhang Jingyu , Wang Hongxun , Lou Weitao , Liu Xiaopeng
Published: May 2018

Thermal barrier coating (TBC) is a critical material in the aerospace domain to increase the lifetime of gas turbine components subjected to thermal load. The properties of TBC are strongly related to the growth of thermally grown oxide (TGO) whose main constituent is Al2O3. However, the oxidation of Cr and Co can affect the growth of TGO, which is not studied sufficiently. In this paper, high-temperature exposure at 1000 °C was performed to investigate the effect of Cr and Co oxides on TGO growth. The morphology and composition analysis of the interface between the ceramic top coat and the bond coat (TC/BC) were investigated by using scanning electron microscopy (SEM) and the energy dispersion spectrum (EDS). The thermodynamics and kinetics of oxidation were analyzed. The results indicated that the oxidation kinetics basically followed the sub-parabolic law with exposure time. Additionally, the major factor affecting the formation of oxides was the diffusion rate at the initial stage of exposure, then oxides depended on thermodynamics, and the oxidation was influenced by both of them in the last stage. The major elements to be oxidized were different at different stages. Moreover, the replacement reaction of Cr2O3 and the phase conversion of Al2O3 resulted in thickness variations of the TGO and Al-depleted zone during high-temperature exposure.
Breakdown Characteristics of Oil-Pressboard Insulation under AC-DC Combined Voltage and Its Mathematical Model
Authors: Chen Qingguo , Zhang Jinfeng , Chi Minghe , Guo Chong
Published: May 2018

An AC-DC combined voltage is applied to the oil-pressboard insulation near the valve side during the operation of a converter transformer. To study the breakdown characteristics of an oil-pressboard insulation under such voltages, a typical plate electrode structure was employed in the laboratory to conduct a breakdown test on the oil-pressboard insulation. The electrical field distribution and the DC contents of the transformer oil and the pressboard in composite insulation under the AC-DC combined voltage were simulated by their dielectric parameters. The breakdown strength of the transformer oil decreases with the increase in the DC content of the applied voltage, whereas that of the pressboard increases. For the oil-pressboard insulation, the breakdown voltage increases first and then decreases. The electric field strength decreases in the transformer oil with the increase in the DC content, whereas it increases in the pressboard. And the DC contents of the transformer and the pressboard in composite insulation were different from that of the applied voltage. Finally, based on the above results, a mathematical model was proposed to describe the breakdown characteristics of the oil-pressboard insulation under the AC-DC combined voltage; the theoretical and experimental results were in good agreement.
Development of Heat Transfer Surface Area Enhancements: A Test Facility for New Heat Exchanger Designs
Authors: Fugmann Hannes , Di Lauro Paolo , Sawant Aniket , Schnabel Lena
Published: May 2018

Enhancing the heat transfer surface by usage of cellular metal structures, such as foams or wire structures, might allow enlarging the surface area, increasing the heat transfer coefficients, decreasing the material utilization, and enabling the flexibility of different geometrical dimensions. However their manufacturing and assembling in a large heat exchanger for performance testing and optimizing can be costly. Therefore a test rig was constructed for experimental characterization of heat transfer surface area enhancements. Heat exchanger samples with dimensions in the centimeter range can be measured. The fluid flow and heat transfer features of a micro pin fin wire structure made from copper by soft-soldering were experimentally characterized under steady-state forced air convection. The results are compared to performance characteristics of louvered fins. Heat transfer coefficients of the pin fins are twice as high as for the louvered fins. The relative expanded uncertainty of the Nusselt number is ±7%.
Evaluation of Salvage Logging Productivity and Costs in Windthrown Norway Spruce-Dominated Forests
Authors: Kärhä Kalle , Anttonen Tuomas , Poikela Asko , Palander Teijo , Laurén Ari , Peltola Heli , Nuutinen Yrjö
Published: May 2018

Different abiotic and biotic disturbances are expected to become more common in the future due to a warming climate. Globally, post-disturbance salvage logging is becoming more predominant to recover economic value from timber in disturbed forests. This study collected comparative time-study data and analyzed the productivity of cutting for windfalls in clear cuttings and determined the cutting costs of windfalls. Furthermore, the logging (i.e., cutting and forwarding) costs of wind-damaged trees and those of undamaged standing Norway spruce (Picea abies (L.) Karst.) trees in clear cuts were calculated in Finland. The results revealed that the cutting productivity of windfalls was 19–33% lower than that of undamaged stems. The cutting costs of windthrown stems with a volume of 0.3–1.5 m3 were 35–64% higher and the logging costs of windfalls were 10–30% higher than those of undamaged standing stems. The study provided new understanding regarding the productivity and costs of salvage logging operations under Finnish conditions. Even if the logging of windfalls is expensive and laborious, salvage logging operations are important for forest stands and their health to minimize post-disaster damage outbreaks in coniferous forests, such as the damage caused by bark beetles—mainly Ips typographus L.
Interactions between Vegetation, Hydrology, and Litter Inputs on Decomposition and Soil CO2 Efflux of Tropical Forests in the Brazilian Pantanal
Authors: Borges Pinto Osvaldo , Vourlitis George , De Souza Carneiro Edna , De França Dias Marizeth , Hentz Cloe , De Souza Nogueira Jose
Published: May 2018

Climate change has the capacity to alter water availability and the litter production of tropical forests, which will alter rates of carbon (C) cycling and storage. We conducted a short-term field experiment in two hydrologically diverse forests in the Brazilian Pantanal to assess the initial response of litter decomposition and soil respiration (Rsoil) to variations in litter pool size. Total annual Rsoil and decomposition significantly declined with litter removal and increased with litter addition, but the rate of litter decomposition was highest for plots where litter was removed. Rsoil was positively related to soil organic matter content and the rate of litter decomposition, but not soil moisture or temperature, suggesting that the litter treatment effects on decomposition and Rsoil were due to changes in C availability and not litter effects on the soil environment (i.e., temperature and moisture). Rsoil was not significantly different between the forests studied here even though they had large differences in hydrology; however, litter decomposition was significantly higher in seasonally flooded forest, especially when augmented with litter. These results suggest that alterations in litter production from land use and/or climate change will alter short-term rates of decomposition and Rsoil for these and other floodplain forests of the Pantanal and Amazon Basin.
Invasive Species May Disrupt Protected Area Networks: Insights from the Pine Wood Nematode Spread in Portugal
Authors: De la Fuente Begoña , Beck Pieter S. A.
Published: May 2018

The expansion of invasive alien species is considered a major threat to forest ecosystems and biodiversity. Their potential impacts range from local changes in species composition to wider-scale effects on forest habitat and landscape functioning, although the latter has been relatively little explored in the literature. Here, we assessed the impact of an invasive forest pest, the pine wood nematode (PWN), in the Natura 2000 network of protected areas (PAs) in Portugal, the first European country in which PWN was reported. We considered the impacts of the pest’s spread (up to 2016) on individual PAs, in terms of the fraction of their coniferous forest infected, and on the corridors between PAs, which were mapped and prioritized through least-cost path modelling, geographic information system analysis, and the graph-based probability of connectivity metric. We found that PWN by 2016 had spread into 49% of the Portuguese Natura 2000 coniferous forest habitat, while it had invaded 68% of the coniferous forests that form the priority corridors between the PAs. These impacts are likely to be aggravated in the next years, given the pace of PWN expansion and the predicted rates of natural spread to new areas in Portugal and, increasingly likely, in Spain. Our results suggest that the connectivity of PA systems may be significantly disrupted by alien species, and that spatially prioritized control measures can help mitigate the impacts of invasive species on the coherence and functionality of protected area networks such as Natura 2000.
Seedling Quality: History, Application, and Plant Attributes
Authors: Grossnickle Steven C. , MacDonald Joanne E.
Published: May 2018

Since the early 20th century, silviculturists have recognized the importance of planting seedlings with desirable attributes, and that these attributes are associated with successful seedling survival and growth after outplanting. Over the ensuing century, concepts on what is meant by a quality seedling have evolved to the point that these assessments now provide value to both the nursery practitioner growing seedlings and the forester planting seedlings. Various seedling quality assessment procedures that measure numerous morphological and physiological plant attributes have been designed and applied. This paper examines the historical development of the discipline of seedling quality, as well as where it is today. It also examines how seedling quality is employed in forest restoration programs and the attributes that are measured to define quality. The intent is to provide readers with an overall perspective on the field of seedling quality and the people who developed this discipline from an idea into an operational reality.
The Influence of Individual-Specific Plant Parameters and Species Composition on the Allergenic Potential of Urban Green Spaces
Authors: Jochner-Oette Susanne , Stitz Theresa , Jetschni Johanna , Cariñanos Paloma
Published: May 2018

Green planning focusses on specific site requirements such as temperature tolerance or aesthetics as crucial criteria in the choice of plants. The allergenicity of plants, however, is often neglected. Cariñanos et al. (2014; Landscape and Urban Planning, 123: 134–144) developed the Urban Green Zone Allergenicity Index (IUGZA) that considers a variety of plant characteristics to calculate the allergenic potential of urban green spaces. Based on this index, we calculated an index for the individual-specific allergenic potential (IISA) that accounts for a varying foliage volume by accurate measurements of crown heights and surface areas occupied by each tree and only included mature individuals. The studied park, located in Eichstätt, Germany, has an area of 2.2 ha and consists of 231 trees. We investigated the influence of species composition using six planting scenarios and analysed the relationship between allergenic potential and species diversity using Shannon index. Only a small number of trees was female and therefore characterised as non-allergenic, 9% of the trees were classified as sources of main local allergens. The allergenic potential of the park based on literature values for crown height and surface was IUGZA = 0.173. Applying our own measurements resulted in IISA = 0.018. The scenarios indicated that replacing trees considered as sources of main local allergens has the strongest impact on the park’s allergenic potential. The IUGZA offers an easy way to assess the allergenic potential of a park by the use of a few calculations. The IISA reduces the high influence of the foliage volume but there are constraints in practicability and in speed of the analysis. Although our study revealed that a greater biodiversity was not necessarily linked to lower index values, urban green planning should focus on biodiversity for ameliorating the allergenic potential of parks.
Validation of Ion TorrentTM Inherited Disease Panel with the PGMTM Sequencing Platform for Rapid and Comprehensive Mutation Detection
Authors: Mustafa Abeer E. , Faquih Tariq , Baz Batoul , Kattan Rana , Al-Issa Abdulelah , Tahir Asma I. , Imtiaz Faiqa , Ramzan Khushnooda , Al-Sayed Moeenaldeen , Alowain Mohammed , Al-Hassnan Zuhair , Al-Zaidan Hamad , Abouelhoda Mohamed , Al-Mubarak Bashayer R. , Al Tassan Nada A.
Published: May 2018

Quick and accurate molecular testing is necessary for the better management of many inherited diseases. Recent technological advances in various next generation sequencing (NGS) platforms, such as target panel-based sequencing, has enabled comprehensive, quick, and precise interrogation of many genetic variations. As a result, these technologies have become a valuable tool for gene discovery and for clinical diagnostics. The AmpliSeq Inherited Disease Panel (IDP) consists of 328 genes underlying more than 700 inherited diseases. Here, we aimed to assess the performance of the IDP as a sensitive and rapid comprehensive gene panel testing. A total of 88 patients with inherited diseases and causal mutations that were previously identified by Sanger sequencing were randomly selected for assessing the performance of the IDP. The IDP successfully detected 93.1% of the mutations in our validation cohort, achieving high overall gene coverage (98%). The sensitivity for detecting single nucleotide variants (SNVs) and short Indels was 97.3% and 69.2%, respectively. IDP, when coupled with Ion Torrent Personal Genome Machine (PGM), delivers comprehensive and rapid sequencing for genes that are responsible for various inherited diseases. Our validation results suggest the suitability of this panel for use as a first-line screening test after applying the necessary clinical validation.
Clinical Efficacy Associated with Enhanced Antioxidant Enzyme Activities of Silver Nanoparticles Biosynthesized Using Moringa oleifera Leaf Extract, Against Cutaneous Leishmaniasis in a Murine Model of Leishmania major
Authors: El-khadragy Manal , Alolayan Ebtesam M. , Metwally Dina M. , El-Din Mohamed F. Serag , Alobud Sara S. , Alsultan Nour I. , Alsaif Sarah S. , Awad Manal A. , Abdel Moneim Ahmed E.
Published: May 2018

Leishmaniasis is one of the most significant vector-borne syndromes of individuals. This parasitic infection can be affected by many species of Leishmania, most of which are zoonotic. Natural products have made and are continuing to make important contributions to the search for new antileishmanial agents. The use of plants in the production assembly of silver nanoparticles has drawn attention because of its rapid, eco-friendly, non-pathogenic, economical protocol and provides a single step technique for the biosynthetic process. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using Moringa oleifera leaf extract and investigated the antileishmanial activity of these nanoparticles in a murine model of Leishmania major infection. A total of 50 mice were used and divided into five groups—healthy control, infected, infected mice treated with pentostam, infected mice treated with Ag-NPs and infected mice pretreated with Ag-NPs. In the present study, the leaf extract of the plant species Moringa oleifera was found to be a good source for the synthesis of silver nanoparticles, their formation being confirmed by color change and stability in solution. In the present murine model of Leishmania major infection, we found that oral treatment with silver nanoparticles biosynthesized using Moringa oleifera extract resulted in a significant reduction in the average size of leishmaniasis cutaneous lesions compared with untreated mice. Furthermore, the clinical efficacy of Moringa oleifera extract was associated with enhanced antioxidant enzyme activities. In conclusion, treatment with silver nanoparticles biosynthesized using Moringa oleifera extract has higher and faster clinical efficacy than standard pentavalent antimonial treatment, probably by boosting the endogenous antioxidant activity.
Physical Activity Recommendations for Health and Beyond in Currently Inactive Populations
Authors: Füzéki Eszter , Banzer Winfried
Published: May 2018

Widespread persistent inactivity makes continued efforts in physical activity promotion a persistent challenge. The precise content of physical activity recommendations is not broadly known, and there are concerns that the general messaging of the guidelines, including the recommendations to perform at least 150 min of at least moderate intensity physical activity per week might seem unattainable for and even actually discourage currently inactive people. Here we show that there are a myriad of ways of being physically active, and provide (in part) out-of-the-box examples of evidence based, pragmatic, easily accessible physical activity regimes below 150 min and/or with lower than moderate intensity that yield meaningful health benefits for currently inactive people.
Effect of Sewage Irrigation on the CT-Measured Soil Pore Characteristics of a Clay Farmland in Northern China
Authors: Guo Xiaoming , Zhao Tongqian , Liu Lin , Xiao Chunyan , He Yuxiao
Published: May 2018

Sewage irrigation has a strong influence on the physical, chemical, and biological properties of soil. However, the effects of sewage irrigation on the pore characteristics of soil are not well understood. This study compares the effects of sewage irrigation and groundwater irrigation on computed tomography (CT)-measured pore parameters and examines the relationships between CT-measured pore parameters and soil physicochemical and microbial properties. Intact soil cores were collected from S1 irrigated with sewage for 25 years, S2 irrigated with sewage for 52 years, and CK irrigated with groundwater. Various soil pore characteristics were determined, including the total pore number, macropore number (>1 mm diam.), coarse mesopore number (0.264–1 mm diam.), total porosity, macroporosity, coarse mesoporosity, and circularity. The results indicated that sewage irrigation significantly affected soil pore number and porosity. Compared with S1 and S2, CK exhibited a higher average total pore number (91), macropore number (40), coarse mesopore number (51), total porosity (2.08%), macroporosity (1.90%), and coarse mesoporosity (0.18%) throughout the 50–350 mm layer. At depths of 200–350 mm, S2 exhibited the lowest average total pore number (33), macropore number (13), coarse mesopore number (21), total porosity (0.42%), macroporosity (0.35%), and coarse mesoporosity (0.07%) among the three sites. In addition, the average pore numbers and porosity at depths of 200–350 mm decreased with increasing sewage irrigation time. There were significant positive correlations between pore features (including pore numbers and porosity) and soil properties (phosphorus content and fungi numbers). Our results suggest that decreased macropore numbers and macroporosity in the sewage-irrigated farmland may strongly intensify the accumulation of metals and nutrients in the upper layer. The findings of this study are useful for understanding the negative effects of sewage irrigation on soil pore structure and are critical for developing sustainable strategies in agriculture.
Reconciling the Entomological Hazard and Disease Risk in the Lyme Disease System
Authors: McClure Max , Diuk-Wasser Maria
Published: May 2018

Lyme disease (LD) is a commonly cited model for the link between habitat loss and/or fragmentation and disease emergence, based in part on studies showing that forest patch size is negatively related to LD entomological risk. An equivalent relationship has not, however, been shown between patch size and LD incidence (LDI). Because entomological risk is measured at the patch scale, while LDI is generally assessed in relation to aggregate landscape statistics such as forest cover, we posit that the contribution of individual patches to human LD risk has not yet been directly evaluated. We design a model that directly links theoretical entomological risk at the patch scale to larger-scale epidemiological data. We evaluate its predictions for relative LD risk in artificial landscapes with varying composition and configuration, and test its ability to predict countywide LDI in a 12-county region of New York. On simulated landscapes, we find that the model predicts a unimodal relationship between LD incidence and forest cover, mean patch size, and mean minimum distance (a measure of isolation), and a protective effect for percolation probability (a measure of connectivity). In New York, risk indices generated by this model are significantly related to countywide LDI. The results suggest that the lack of concordance between entomological risk and LDI may be partially resolved by this style of model.
Dopamine Receptor Subtypes Differentially Regulate Autophagy
Authors: Wang Dongmei , Ji Xinmiao , Liu Juanjuan , Li Zhiyuan , Zhang Xin
Published: May 2018

Some dopamine receptor subtypes were reported to participate in autophagy regulation, but their exact functions and mechanisms are still unclear. Here we found that dopamine receptors D2 and D3 (D2-like family) are positive regulators of autophagy, while dopamine receptors D1 and D5 (D1-like family) are negative regulators. Furthermore, dopamine and ammonia, the two reported endogenous ligands of dopamine receptors, both can induce dopamine receptor internalization and degradation. In addition, we found that AKT (protein kinase B)-mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) pathways are involved in DRD3 (dopamine receptor D3) regulated autophagy. Moreover, autophagy machinery perturbation inhibited DRD3 degradation and increased DRD3 oligomer. Therefore, our study investigated the functions and mechanisms of dopamine receptors in autophagy regulation, which not only provides insights into better understanding of some dopamine receptor-related neurodegeneration diseases, but also sheds light on their potential treatment in combination with autophagy or mTOR pathway modulations.
Role of mTOR Complexes in Neurogenesis
Authors: LiCausi Francesca , Hartman Nathaniel W.
Published: May 2018

Dysregulation of neural stem cells (NSCs) is associated with several neurodevelopmental disorders, including epilepsy and autism spectrum disorder. The mammalian target of rapamycin (mTOR) integrates the intracellular signals to control cell growth, nutrient metabolism, and protein translation. mTOR regulates many functions in the development of the brain, such as proliferation, differentiation, migration, and dendrite formation. In addition, mTOR is important in synaptic formation and plasticity. Abnormalities in mTOR activity is linked with severe deficits in nervous system development, including tumors, autism, and seizures. Dissecting the wide-ranging roles of mTOR activity during critical periods in development will greatly expand our understanding of neurogenesis.
ENABLE 2017, the First EUROPEAN PhD and Post-Doc Symposium. Session 3: In Vitro to In Vivo: Modeling Life in 3D
Authors: Di Mauro Gianmarco , Dondi Ambra , Giangreco Giovanni , Hogrebe Alexander , Louer Elja , Magistrati Elisa , Mullari Meeli , Turon Gemma , Verdurmen Wouter , Cortada Helena Xicoy , Zivanovic Sanja
Published: May 2018

The EUROPEAN ACADEMY FOR BIOMEDICAL SCIENCE (ENABLE) is an initiative funded by the European Union Horizon 2020 program involving four renowned European research institutes (Institute for Research in Biomedicine—IRB Barcelona, Spain; Radboud Institute for Molecular Life Sciences—RIMLS, the Netherlands; Novo Nordisk Foundation Center for Protein Research—NNF CPR, Denmark; European School of Molecular Medicine—SEMM, Italy) and an innovative science communication agency (Scienseed). With the aim to promote biomedical science of excellence in Europe, ENABLE organizes an annual three-day international event. This gathering includes a top-level scientific symposium bringing together leading scientists, PhD students, and post-doctoral fellows; career development activities supporting the progression of young researchers and fostering discussion about opportunities beyond the bench; outreach activities stimulating the interaction between science and society. The first European PhD and Postdoc Symposium, entitled “Breaking Down Complexity: Innovative models and techniques in biomedicine”, was hosted by the vibrant city of Barcelona. The scientific program of the conference was focused on the most recent advances and applications of modern techniques and models in biomedical research and covered a wide range of topics, from synthetic biology to translational medicine. Overall, the event was a great success, with more than 200 attendees from all over Europe actively participating in the symposium by presenting their research and exchanging ideas with their peers and world-renowned scientists.
Modeling the Effect of Primary and Secondary Twinning on Texture Evolution during Severe Plastic Deformation of a Twinning-Induced Plasticity Steel
Authors: Toth Laszlo S. , Haase Christian , Allen Robert , Lapovok Rimma , Molodov Dmitri A. , Cherkaoui Mohammed , Kadiri Haitham El
Published: May 2018

Modeling the effect of deformation twinning and the ensuing twin-twin- and slip-twin-induced hardening is a long-standing problem in computational mechanical metallurgy of materials that deform by both slip and twinning. In this work, we address this effect using the twin volume transfer method, which obviates the need of any cumbersome criterion for twin variant selection. Additionally, this method is capable of capturing, at the same time, secondary or double twinning, which is particularly important for modeling in large strain regimes. We validate our modeling methodology by simulating the behavior of an Fe-23Mn-1.5Al-0.3C twinning-induced plasticity (TWIP) steel under large strain conditions, experimentally achieved in this work through equal-channel angular pressing (ECAP) for up to two passes in a 90° die following route BC at 300 °C. Each possible twin variant, whether nucleating inside the parent grain or inside a potential primary twin variant was predefined in the initial list of orientations as possible grain of the polycrystal with zero initial volume fraction. A novelty of our approach is to take into account the loss of coherency of the twins with their parent matrix under large strains, obstructing progressively their further growth. This effect has been captured by attenuating growth rates of twins as a function of their rotation away from their perfect twin orientation, dubbed here as “disorientation” with respect to the mother grain’s lattice. The simulated textures and the hardening under tensile strain showed very good agreement with experimental characterization and mechanical testing results. Furthermore, upper-bound Taylor deformation was found to be operational for the TWIP steel deformation when all the above ingredients of twinning are captured, indicating that self-consistent schemes can be bypassed.
Removal of Crystal Violet by Using Reduced-Graphene-Oxide-Supported Bimetallic Fe/Ni Nanoparticles (rGO/Fe/Ni): Application of Artificial Intelligence Modeling for the Optimization Process
Authors: Ruan Wenqian , Hu Jiwei , Qi Jimei , Hou Yu , Cao Rensheng , Wei Xionghui
Published: May 2018

Reduced-graphene-oxide-supported bimetallic Fe/Ni nanoparticles were synthesized in this study for the removal of crystal violet (CV) dye from aqueous solutions. This material was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS), Raman spectroscopy, N2-sorption, and X-ray photoelectron spectroscopy (XPS). The influence of independent parameters (namely, initial dye concentration, initial pH, contact time, and temperature) on the removal efficiency were investigated via Box–Behnken design (BBD). Artificial intelligence (i.e., artificial neural network, genetic algorithm, and particle swarm optimization) was used to optimize and predict the optimum conditions and obtain the maximum removal efficiency. The zero point of charge (pHZPC) of rGO/Fe/Ni composites was determined by using the salt addition method. The experimental equilibrium data were fitted well to the Freundlich model for the evaluation of the actual behavior of CV adsorption, and the maximum adsorption capacity was estimated as 2000.00 mg/g. The kinetic study discloses that the adsorption processes can be satisfactorily described by the pseudo-second-order model. The values of Gibbs free energy change (ΔG0), entropy change (ΔS0), and enthalpy change (ΔH0) demonstrate the spontaneous and endothermic nature of the adsorption of CV onto rGO/Fe/Ni composites.
Superhydrophobic Natural and Artificial Surfaces—A Structural Approach
Authors: Avrămescu Roxana-Elena , Ghica Mihaela Violeta , Dinu-Pîrvu Cristina , Prisada Răzvan , Popa Lăcrămioara
Published: May 2018

Since ancient times humans observed animal and plants features and tried to adapt them according to their own needs. Biomimetics represents the foundation of many inventions from various fields: From transportation devices (helicopter, airplane, submarine) and flying techniques, to sports’ wear industry (swimming suits, scuba diving gear, Velcro closure system), bullet proof vests made from Kevlar etc. It is true that nature provides numerous noteworthy models (shark skin, spider web, lotus leaves), referring both to the plant and animal kingdom. This review paper summarizes a few of “nature’s interventions” in human evolution, regarding understanding of surface wettability and development of innovative special surfaces. Empirical models are described in order to reveal the science behind special wettable surfaces (superhydrophobic /superhydrophilic). Materials and methods used in order to artificially obtain special wettable surfaces are described in correlation with plants’ and animals’ unique features. Emphasis is placed on joining superhydrophobic and superhydrophilic surfaces, with important applications in cell culturing, microorganism isolation/separation and molecule screening techniques. Bio-inspired wettability is presented as a constitutive part of traditional devices/systems, intended to improve their characteristics and extend performances.
Extreme Ultraviolet to Visible Dispersed Single Photon Detection for Highly Sensitive Sensing of Fundamental Processes in Diverse Samples
Authors: Hans Andreas , Schmidt Philipp , Ozga Christian , Hartmann Gregor , Holzapfel Xaver , Ehresmann Arno , Knie André
Published: May 2018

The detection of a single photon is the most sensitive method for sensing of photon emission. A common technique for single photon detection uses microchannel plate arrays combined with photocathodes and position sensitive anodes. Here, we report on the combination of such detectors with grating diffraction spectrometers, constituting a low-noise wavelength resolving photon spectroscopy apparatus with versatile applicability. We recapitulate the operation principle of such detectors and present the details of the experimental set-up, which we use to investigate fundamental mechanisms in atomic and molecular systems after excitation with tuneable synchrotron radiation. Extensions for time and polarization resolved measurements are described and examples of recent applications in current research are given.
Mass Transfer in Osmotic Dehydration of Kiwiberry: Experimental and Mathematical Modelling Studies
Authors: Bialik Michał , Wiktor Artur , Latocha Piotr , Gondek Ewa
Published: May 2018

The aim of this study was to analyze the impact of osmotic solutions and temperature on the osmotic dehydration (OD) of two cultivars of kiwiberry. OD was carried out in sucrose, xylitol and maltitol solutions at 30 °C and 50 °C, respectively. The process of osmotic dehydration was described by the means of water loss (WL), solid gain (SG), weight reduction (WR), and water content changes. Moreover, dehydration was described by mathematical models often used in the literature. The highest WL, WR and SG values were observed for samples treated by xylitol and maltitol at 50 °C. The statistical analysis of the mathematical modelling of the process showed that in most cases, the Peleg’s equation exhibits better fitting for the experimental data.
Design and Efficacy of Nanogels Formulations for Intranasal Administration
Authors: Aderibigbe Blessing A. , Naki Tobeka
Published: May 2018

Nanogels are drug delivery systems that can bypass the blood-brain barrier and deliver drugs to the desired site when administered intranasally. They have been used as a drug delivery platform for the management of brain diseases such as Alzheimer disease, migraine, schizophrenia and depression. nanogels have also been developed as vaccine carriers for the protection of bacterial infections such as influenza, meningitis, pneumonia and as veterinary vaccine carriers for the protection of animals from encephalomyelitis and mouth to foot disease. It has been developed as vaccine carriers for the prevention of lifestyle disease such as obesity. Intranasal administration of therapeutics using nanogels for the management of brain diseases revealed that the drug transportation was via the olfactory nerve pathway resulting in rapid drug delivery to the brain with excellent neuroprotective effect. The application of nanogels as vaccine carriers also induced significant responses associated with protective immunity against selected bacterial and viral infections. This review provides a detailed information on the enhanced therapeutic effects, mechanisms and biological efficacy of nanogels for intranasal administration.
Properties of Ferrite Garnet (Bi, Lu, Y)3(Fe, Ga)5O12 Thin Film Materials Prepared by RF Magnetron Sputtering
Authors: Nur-E-Alam Mohammad , Vasiliev Mikhail , Belotelov Vladimir , Alameh Kamal
Published: May 2018

This work is devoted to physical vapor deposition synthesis, and characterisation of bismuth and lutetium-substituted ferrite-garnet thin-film materials for magneto-optic (MO) applications. The properties of garnet thin films sputtered using a target of nominal composition type Bi0.9Lu1.85Y0.25Fe4.0Ga1O12 are studied. By measuring the optical transmission spectra at room temperature, the optical constants and the accurate film thicknesses can be evaluated using Swanepoel’s envelope method. The refractive index data are found to be matching very closely to these derived from Cauchy’s dispersion formula for the entire spectral range between 300 and 2500 nm. The optical absorption coefficient and the extinction coefficient data are studied for both the as-deposited and annealed garnet thin-film samples. A new approach is applied to accurately derive the optical constants data simultaneously with the physical layer thickness, using a combination approach employing custom-built spectrum-fitting software in conjunction with Swanepoel’s envelope method. MO properties, such as specific Faraday rotation, MO figure of merit and MO swing factor are also investigated for several annealed garnet-phase films.
Choline Supplementation Prevents a Hallmark Disturbance of Kwashiorkor in Weanling Mice Fed a Maize Vegetable Diet: Hepatic Steatosis of Undernutrition
Authors: May Thaddaeus , Klatt Kevin C. , Smith Jacob , Castro Eumenia , Manary Mark , Caudill Marie A. , Jahoor Farook , Fiorotto Marta L.
Published: May 2018

Hepatic steatosis is a hallmark feature of kwashiorkor malnutrition. However, the pathogenesis of hepatic steatosis in kwashiorkor is uncertain. Our objective was to develop a mouse model of childhood undernutrition in order to test the hypothesis that feeding a maize vegetable diet (MVD), like that consumed by children at risk for kwashiorkor, will cause hepatic steatosis which is prevented by supplementation with choline. A MVD was developed with locally sourced organic ingredients, and fed to weanling mice (n = 9) for 6 or 13 days. An additional group of mice (n = 4) were fed a choline supplemented MVD. Weight, body composition, and liver changes were compared to control mice (n = 10) at the beginning and end of the study. The MVD resulted in reduced weight gain and hepatic steatosis. Choline supplementation prevented hepatic steatosis and was associated with increased hepatic concentrations of the methyl donor betaine. Our findings show that (1) feeding a MVD to weanling mice rapidly induces hepatic steatosis, which is a hallmark disturbance of kwashiorkor; and that (2) hepatic steatosis associated with feeding a MVD is prevented by choline supplementation. These findings support the concept that insufficient choline intake may contribute to the pathogenesis of hepatic steatosis in kwashiorkor.
Circulating Metabolites Associated with Alcohol Intake in the European Prospective Investigation into Cancer and Nutrition Cohort
Authors: Van Roekel Eline H. , Trijsburg Laura , Assi Nada , Carayol Marion , Achaintre David , Murphy Neil , Rinaldi Sabina , Schmidt Julie A. , Stepien Magdalena , Kaaks Rudolf , Kühn Tilman , Boeing Heiner , Iqbal Khalid , Palli Domenico , Krogh Vittorio , Tumino Rosario , Ricceri Fulvio , Panico Salvatore , Peeters Petra H. , Bueno-de-Mesquita Bas , Ardanaz Eva , Lujan-Barroso Leila , Quirós J. Ramón , Huerta José M. , Molina-Portillo Elena , Dorronsoro Miren , Tsilidis Konstantinos K. , Riboli Elio , Rostgaard-Hansen Agnetha Linn , Tjønneland Anne , Overvad Kim , Weiderpass Elisabete , Boutron-Ruault Marie-Christine , Severi Gianluca , Trichopoulou Antonia , Karakatsani Anna , Kotanidou Anastasia , Håkansson Anders , Malm Johan , Weijenberg Matty P. , Gunter Marc J. , Jenab Mazda , Johansson Mattias , Travis Ruth C. , Scalbert Augustin , Ferrari Pietro
Published: May 2018

Identifying the metabolites associated with alcohol consumption may provide insights into the metabolic pathways through which alcohol may affect human health. We studied associations of alcohol consumption with circulating concentrations of 123 metabolites among 2974 healthy participants from the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Alcohol consumption at recruitment was self-reported through dietary questionnaires. Metabolite concentrations were measured by tandem mass spectrometry (BIOCRATES AbsoluteIDQTM p180 kit). Data were randomly divided into discovery (2/3) and replication (1/3) sets. Multivariable linear regression models were used to evaluate confounder-adjusted associations of alcohol consumption with metabolite concentrations. Metabolites significantly related to alcohol intake in the discovery set (FDR q-value < 0.05) were further tested in the replication set (Bonferroni-corrected p-value < 0.05). Of the 72 metabolites significantly related to alcohol intake in the discovery set, 34 were also significant in the replication analysis, including three acylcarnitines, the amino acid citrulline, four lysophosphatidylcholines, 13 diacylphosphatidylcholines, seven acyl-alkylphosphatidylcholines, and six sphingomyelins. Our results confirmed earlier findings that alcohol consumption was associated with several lipid metabolites, and possibly also with specific acylcarnitines and amino acids. This provides further leads for future research studies aiming at elucidating the mechanisms underlying the effects of alcohol in relation to morbid conditions.
Electrospun Antimicrobial Polylactic Acid/Tea Polyphenol Nanofibers for Food-Packaging Applications
Authors: Liu Yaowen , Liang Xue , Wang Shuyao , Qin Wen , Zhang Qing
Published: May 2018

The development of new bioactive food-packaging materials that extend the shelf life of food is an important objective. Herein, we report the fabrication of four polylactic acid/tea polyphenol (PLA/TP) composite nanofibers, with PLA/TP ratios of 5:1, 4:1, 3:1, and 2:1, by electrospinning. The morphological quality of each sample was examined by scanning electron microscopy (SEM), and samples with higher TP content were found to be deeper in color. The samples were then examined by Fourier transform infrared (FTIR) spectroscopy to confirm the presence of TP. Examination of the mechanical properties of these fibers revealed that the presence of TP decreased both tensile strength and elongation at break; however, this decrease was only slight for the PLA/TP-3:1 composite fiber. The addition of TP influenced the hydrophilic–hydrophobic property and release behavior of the composite fibers, which significantly improved the antioxidant behavior of these samples, with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacities of up to 95.07% ± 10.55% observed. Finally, antimicrobial activities against Escherichia coli and Staphylococcus aureus of up to 92.26% ± 5.93% and 94.58% ± 6.53%, respectively, were observed for the PLA/TP-3:1 composite fiber. The present study demonstrated that PLA/TP composite nanofibers can potentially be used for food-packaging applications that extend food shelf life.