bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychophysical Studies of Interleaving Narrowband Tactile Stimuli to Achieve Broadband Perceptual Effects
Authors: Martinez Juan S. , Tan Hong Z. , Cholewiak Roger W.
Published: May 2022

Despite the ubiquitous presence of tactile actuators (tactors) in mobile devices, there is a continuing need for more advanced tactors that can cover the entire frequency range of human tactile perception. Broadband tactors can increase information transmission and enrich sensory experience. The engineering challenges are multifold in that the ideal tactors should exhibit an effective bandwidth of at least 300 Hz, small form factor, robustness, power efficiency and low cost. For wearable applications, there are the additional challenges of ease of mounting and maintaining adequate skin contact during body movements. We propose an approach to interleave narrowband tactile stimuli to achieve broadband effects, taking advantage of the limited spatial resolution of the skin on the torso and limbs. Three psychophysical experiments were conducted to assess the validity of this approach. Participants performed pairwise discriminations of two broadband stimuli delivered using one or two tactors. The broadband stimuli consisted of one mid-frequency and one high-frequency component delivered through one tactor by mixing the two components, or through two tactors (one component per tactor). The first two experiments revealed extraneous cues such as localization and mutual masking of mid- and high-frequency components that were subsequently eliminated in the third experiment. Results from 12 participants confirmed that performance on pairwise comparisons was below the discrimination threshold, confirming that broadband haptic effects can be achieved through narrowband tactors placed within the skin’s two-point limen.
Relationship Between Particle Properties and Immunotoxicological Effects of Environmentally-Sourced Microplastics
Authors: Beijer Nick R. M. , Dehaut Alexandre , Carlier Maxim P. , Wolter Helen , Versteegen Ron M. , Pennings Jeroen L. A. , de la Fonteyne Liset , Niemann Helge , Janssen Henk M. , Timmermans Belinda G. , Mennes Wim , Cassee Flemming R. , Mengelers Marcel J. B. , Amaral-Zettler Linda A. , Duflos Guillaume , Staal Yvonne C. M.
Published: May 2022

Background

Concerns on microplastics (MPs) in food are increasing because of our increased awareness of daily exposure and our knowledge gap on their potential adverse health effects. When particles are ingested, macrophages play an important role in scavenging them, potentially leading to an unwanted immune response. To elucidate the adverse effects of MPs on human health, insights in the immunotoxicity of MPs are essential.

Objectives

To assess the effect of environmentally collected ocean and land weathered MP particles on the immunological response of macrophages using a state-of-the art in vitro immunotoxicity assay specifically designed for measuring particle toxicity.

Methods

Environmentally-weathered macroplastic samples were collected from the North Pacific Subtropical Gyre and from the French coastal environment. Macroplastics were identified using (micro)Raman-spectrometry, FT-IR and Py-GC-MS and cryo-milled to obtain size-fractionated samples up to 300 μm. Physiochemical MP properties were characterized using phase contrast microscopy, gel-permeation chromatography, nuclear magnetic resonance, and differential scanning colorimetry. Macrophages (differentiated THP-1 cells) were exposed to particles (<300 μm) for 48 h before assessment of cell viability and cytokine release. Using both the physiochemical particle properties and biological data, we performed multi-dimensional data analysis to explore relationships between particle properties and immunotoxicological effects.

Results

We investigated land-derived polyethylene, polypropylene, polystyrene, and polyethylene terephthalate, water-derived polypropylene macroplastics, and virgin polyethylene fibers and nylon MPs. The different plastic polymeric compositions and MP size classes induced distinct cytokine responses. Macrophages had the largest response to polyethylene terephthalate-particle exposure, including a dose-related increase in IL-1β, IL-8, and TNF-α secretion. Smaller MPs induced cytokine production at lower concentrations. Additionally, a relationship between both physical and chemical particle properties and the inflammatory response of macrophages was found.

Discussion

This research shows that MP exposure could lead to an inflammatory response in vitro, depending on MP material and size. Whether this implies a risk to human health needs to be further explored.
Eruption and Interplanetary Evolution of a Stealthy Streamer-Blowout CME Observed by PSP at ∼0.5 AU
Authors: Pal Sanchita , Lynch Benjamin J. , Good Simon W. , Palmerio Erika , Asvestari Eleanna , Pomoell Jens , Stevens Michael L. , Kilpua Emilia K. J.
Published: May 2022

Streamer-blowout coronal mass ejections (SBO-CMEs) are the dominant CME population during solar minimum. Although they are typically slow and lack clear low-coronal signatures, they can cause geomagnetic storms. With the aid of extrapolated coronal fields and remote observations of the off-limb low corona, we study the initiation of an SBO-CME preceded by consecutive CME eruptions consistent with a multi-stage sympathetic breakout scenario. From inner-heliospheric Parker Solar Probe (PSP) observations, it is evident that the SBO-CME is interacting with the heliospheric magnetic field and plasma sheet structures draped about the CME flux rope. We estimate that 18 ± 11% of the CME’s azimuthal magnetic flux has been eroded through magnetic reconnection and that this erosion began after a heliospheric distance of ∼0.35 AU from the Sun was reached. This observational study has important implications for understanding the initiation of SBO-CMEs and their interaction with the heliospheric surroundings.
Impact of Natural Farming Cropping System on Rural Households—Evidence From Solan District of Himachal Pradesh, India
Authors: Laishram Chinglembi , Vashishat Rohit Kumar , Sharma Subhash , Rajkumari Bhavna , Mishra Neha , Barwal Parul , Vaidya Manoj Kumar , Sharma Ravinder , Chandel Rajeshwar Singh , Chandel Ashu , Gupta Rakesh Kumar , Sharma Neha
Published: May 2022

Natural farming, popularly known as zero budget natural farming, is an innovative farming approach. It is low input based, climate resilient, and low cost farming system because all the inputs (insect repellents, fungicides, and pesticides) are made up of natural herbs and locally available inputs, thereby reducing the use of artificial fertilizers and industrial pesticides. It is becoming increasingly popular among the smallholder farmers of Himachal Pradesh. Under the natural farming system, 3 to 12 crops are cultivated together on the same area, along with leguminous crops as intercrop in order to ensure that no piece of land is wasted and utilized properly. This article focuses mainly on the different cropping systems of natural farming and comparing the economics of natural farming (NF) with conventional farming (CF) systems. Study shows that farmers adopted five major crop combinations under natural farming system, i.e., vegetables-based cropping system (e.g., tomato + beans + cucumber and cauliflower + pea + radish), vegetables-cereals-based cropping system, and other three more cropping systems discussed in this article. The results indicated that a vegetable-based cropping system has 19.68% more net return in Kharif season and 24.64% more net return in Rabi season as compared to conventional farming vegetable-based monocropping system. NF maximizes land use and reduces the chance of crop yield loss. NF has resulted in increased returns especially in the vegetable cropping system where reduction in cost was 30.73 per cent (kharif) and 11.88 per cent (rabi) across all crop combinations in comparison to CF. It is found in study that NF was cost savings from not using chemical fertilizers and pesticides, as well as higher benefit from intercrops.
Safety and Feasibility of Esophagectomy Following Neoadjuvant Immunotherapy Combined with Chemotherapy for Esophageal Squamous Cell Carcinoma
Authors: Gu Yi-Min , Shang Qi-Xin , Zhang Han-Lu , Yang Yu-Shang , Wang Wen-Ping , Yuan Yong , Hu Yang , Che Guo-Wei , Chen Long-Qi
Published: May 2022

Background

This study aimed to investigate the safety and feasibility of esophagectomy after neoadjuvant immunotherapy and chemotherapy for esophageal squamous cell carcinoma.

Methods

We retrospectively identified patients who received neoadjuvant immunotherapy combined with chemotherapy (n = 38) in our center between 2020 and 2021. The primary end point was the risk of major complications (grade ≥3) according to the Clavien–Dindo classification. Secondary end points were surgical details, 30-day mortality, and 30-day readministration.

Results

The most commonly used regimens of immunotherapy were camrelizumab (36.8%), pembrolizumab (31.5%), tislelizumab (15.8%), sintilimab (13.2%), and toripalimab (2.6%). The median interval to surgery was 63 days (range, 40–147). Esophagectomy was performed in 37 of 38 patients who received neoadjuvant immunotherapy and chemotherapy. All procedures were performed minimally invasively, except for 1 patient who was converted to thoracotomy. Of 37 surgical patients, R0 resection was achieved in 36 patients (97.3%). Pathologic complete response was observed in 9 patients (24.3%). Tumor regression grade I was identified in 17 patients (45.9%). Morbidity occurred in 12 of 37 patients (32.4%). The most common complication was pneumonia (16.2%). There were no deaths or readministration within 30 days.

Conclusions

Esophagectomy following neoadjuvant immune checkpoint inhibitor plus chemotherapy for patients with resectable esophageal squamous cell carcinoma appears to be safe and feasible, with acceptable complication rates.
The Guidewire-assisted Drainage Catheter Placement in Chronic Subdural Hematoma
Authors: Zheng Bingjie , Wang Chunlei , Yao Jinbiao , Zhu Shiyi , Meshcheryakova Svetlana , Shi Huaizhang
Published: May 2022

Background

Chronic subdural hematoma (cSDH) is a common neurosurgical pathology associated with older age. The burr hole drainage is a predominant technique with a lower incidence of recurrence and morbidity. The blind placement of the subdural drain could result in intracerebral hemorrhage. This paper describes a simple and reliable technique for drainage catheter placement in cSDH to reduce intracerebral hemorrhage.

Methods

Forty-nine consecutive patients with cSDH were treated with The Guidewire-assisted Drainage Catheter Placement Technique between July 2019 and June 2021. Epidemiological, clinical and radiographical data were collected and reviewed. The operative technique consists of an angular guidewire tip and catheter. Under the navigation of the guidewire, the catheter is inserted into the subdural space and the length of catheter remaining in the subdural space was 4–5 cm. The catheter was tunneled subcutaneously and fixed at the point where it emerged from the scalp.

Results

Forty-nine consecutive patients underwent 55 The Guidewire-assisted Drainage Catheter Placement. The gender distribution was 37 men and 12 women. The mean age was 69.3 years. The patients presented with headache (31 patients), weakness of limbs (28 patients), speech disturbances (7 patients), and Altered behavior (6 patients). Neither intracerebral hemorrhages nor post-operative seizure occurred. Forty-seven patients were improved after the operation. The recurrence occurred in one patient.

Conclusions

The Guidewire-assisted Drainage Catheter Placement Technique is a reliable method for the insertion of a subdural catheter to evacuate of the Chronic Subdural Hematoma, and is associated with an extremely low risk to cortical structures and cerebral veins.
Effect Evaluation of Subxiphoid and Intercostal Thymectomy: A Meta-Analysis and Systematic Review
Authors: Wang Hailong , Wang Miao , Xin Ning , Wei Rongqiang , Huang Kenan
Published: May 2022

Background

It still remains unclear whether subxiphoid video-assisted thoracoscopic surgery (SVATS) thymectomy is safe and reasonable. This meta-analysis aims at assessing the effectiveness and safety of SVATS for thymoma in comparison with that of intercostal video-assisted thoracoscopic surgery (IVATS) thymectomy.

Methods

All the relevant data systematically analyzed in this thesis were retrieved from PubMed, the Cochrane Library, web of science, EMBASE, and ClinicalTrials.gov. The time span for data retrieval was from the date of database establishment to March 2022. The outcome indicators include operation time, intraoperative blood loss, duration of postoperative drainage, postoperative hospital days, visual analogue scale (VAS) score on the day of operation, VAS score on postoperative day 3, and VAS score on postoperative day 7; postoperative complications were analyzed in our meta-analysis.

Results

In 13 studies of this paper, there were 1,198 cases included. Among them, 563 cases were treated by SVATS thymectomy and 635 cases by IVATS thymectomy. There was no significant difference in the operation time [113.38 vs. 119.91  min, 95% confidence interval (CI): −0.70–0.15, p = 0.20) and the incidence of intraoperative and postoperative complications (RR = 0.82, 95% CI: 0.58–1.15, p = 0.25) between SVATS thymectomy and IVATS thymectomy. However, SVATS thymectomy significantly reduced the amount of intraoperative blood loss (47.68 vs. 66.69  mL, SMD = −0.57, 95% CI: −0.95 to −0.18, p = 0.004), postoperative drainage days (2.12 vs. 2.72 days, SMD = −0.46, 95% CI: −0.74 to −0.18, p = 0.001), postoperative hospital stays (4.53 vs. 5.91 days, SMD = −0.64, 95% CI: −0.96 to −0.31, p = 0.0001), and VAS scores after the operation.

Discussion

SVATS thymectomy is safe and feasible, and the perioperative effect is better than IVATS thymectomy to a certain extent, which is worthy of popularization and further research.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/
Kinetic Analysis in Horses With Deep Digital Flexor Tendinopathy Within the Digit Diagnosed by Magnetic Resonance Imaging
Authors: Madsen Lori M. , Gutierrez-Nibeyro Santiago D. , Stewart Matthew C. , McCoy Annette M. , Schaeffer David J.
Published: May 2022

Objectives

To determine the stance duration and ground reaction forces (GRF) of horses with deep digital flexor (DDF) tendinopathy at the level of the foot and compare the stance duration and GRF to those of clinically sound horses.

Design

Prospective clinical study.

Animals

Sixteen horses (seven horses with bilateral forelimb lameness, four horses with unilateral forelimb lameness, and five horses with no lameness).

Procedures

Analyses of kinetic variables were performed on both forelimbs from sound horses and horses diagnosed with chronic DDF tendinopathy. Stance duration and longitudinal and vertical components of the GRF were determined for the limbs of clinically sound horses and limbs of horses with DDF tendinopathy. Separate Spearman correlation analyses were used to assess potential association within groups (combined left and right forelimbs of clinically sound horses, lamest limbs of horses with DDF tendinopathy, and contralateral limbs of horses with DDF tendinopathy) and with the set of kinetic variables. Analysis of variance on mean ranks of tied values was used to determine differences in kinetic variables between groups (PROC GLIMMIX) using the kinetic values of the clinically sound horses as the reference group.

Results

There were a total of 11 lame horses. Seven horses had bilateral forelimb lameness and four had unilateral lameness. Of the 11 horses, there were 15 DDF tendinopathies. There were eight dorsal border DDF tendinopathies, five core DDF tendinopathies, and two sagittal/parasagittal splits DDF tendinopathies. The most lame limbs of horses with DDF tendinopathy had significantly smaller values for peak vertical force and time of peak braking force than did forelimbs of clinically sound horses. Also, the most lame limbs of horses with DDF tendinopathy had significantly larger values for the time of peak vertical force than did forelimbs of clinically sound horses.

Conclusions and Clinical Relevance

Horses with chronic DDF tendinopathies develop certain alterations of GRF parameters. This information can be used in future studies to determine if particular kinetic variable changes in horses with DDF tendinopathies differ from those of horses with other pathologies within the foot and therefore could be diagnostic.
Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review
Authors: Bezerra Andréia Santana , Santos Marcos Antônio Souza dos , Lourenço-Júnior José de Brito
Published: May 2022

This study identifies the number of publications that presented technologies used in the production systems of Santa Inês sheep in the last 5 years (2017–2021) carried out in Brazil. Therefore, the objective was to identify where we are in terms of knowledge about technologies in different fields (health, reproduction, animal breeding, behavior and welfare, nutrition and feeding, forage and pasture, carcass and meat quality, and economics and management of livestock systems). After rigorous selection, 114 studies were appointed and classified by knowledge field, and the main approaches within each theme were evaluated, pointing out research gaps. Most technologies have been in northeastern states. However, government agencies should develop public policies to disseminate techniques in rural areas because the production system in this region is still subsistence. This study highlighted the need for works that present management practices and tools that impact the improvement of animal welfare. Agro-industrial by-products have been widely used as an alternative for sheep feeding. However, economic feasibility analyses are recommended with these foodstuffs to substantiate their use as an option to reduce production costs. There is a lack of research allusive to the management of production systems, especially those related to estimates of economic feasibility indicators.
Validation of a Liquid Biopsy Protocol for Canine BRAFV595E Variant Detection in Dog Urine and Its Evaluation as a Diagnostic Test Complementary to Cytology
Authors: Gentilini Fabio , Palgrave Christopher J. , Neta Michal , Tornago Raimondo , Furlanello Tommaso , McKay Jennifer S. , Sacchini Federico , Turba Maria E.
Published: May 2022

A significant proportion of canine urothelial carcinomas carry the driver valine to glutamic acid variation (V595E) in BRAF kinase. The detection of V595E may prove suitable to guide molecularly targeted therapies and support non-invasive diagnosis of the urogenital system by means of a liquid biopsy approach using urine. Three cohorts and a control group were included in this multi-step validation study which included setting up a digital PCR assay. This was followed by investigation of preanalytical factors and two alternative PCR techniques on a liquid biopsy protocol. Finally, a blind study using urine as diagnostic sample has been carried out to verify its suitability as diagnostic test to complement cytology. The digital PCR (dPCR) assay proved consistently specific, sensitive, and linear. Using the dPCR assay, the prevalence of V595E in 22 urothelial carcinomas was 90.9%. When compared with histopathology as gold standard in the blind-label cases, the diagnostic accuracy of using the canine BRAF (cBRAF) variation as a surrogate assay against the histologic diagnosis was 85.7% with 92.3% positive predictive value and 80.0% negative predictive value. In all the cases, in which both biopsy tissue and the associated urine were assayed, the findings matched completely. Finally, when combined with urine sediment cytology examination in blind-label cases with clinical suspicion of malignancy, the dPCR assay significantly improved the overall diagnostic accuracy. A liquid biopsy approach on urine using the digital PCR may be a valuable breakthrough in the diagnostic of urothelial carcinomas in dogs.
The miR-216/miR-217 Cluster Regulates Lipid Metabolism in Laying Hens With Fatty Liver Syndrome via PPAR/SREBP Signaling Pathway
Authors: Zhu Lihui , Liao Rongrong , Huang Jiwen , Yan Huaxiang , Xiao Changfeng , Yang Yunzhou , Wang Huiying , Yang Changsuo
Published: May 2022

Fatty liver syndrome (FLS), a common metabolic disease in laying hens, caused by excessive hepatic fat deposition is a bottleneck in the poultry industry. However, no specific therapeutic methods have been developed. Evidence suggests that microRNAs (miRNAs) are essential for liver lipid metabolism and homeostasis, providing strong evidence for targeting miRNAs as a potential treatment option for liver diseases. However, the roles of miRNAs in the pathogenesis of FLS remain unclear. In present study, RNA-sequencing was performed to discern the expression patterns of miRNAs in normal and fatty livers of laying hens. In total, 12 dysregulated miRNAs (2 down-regulated and 10 up-regulated) were detected between the normal and fatty livers. Functional enrichment analysis showed the potential impacts of the dysregulated miRNAs on lipid metabolism. Notably, miR-216a/b and miR-217-5p, which belong to the miR-216/miR-217 cluster, were up-regulated in the sera and livers of FLS chickens, as well as free fatty acid (FFA)-induced LMH cells. Oil-red O staining revealed that up-regulation of the miR-216/miR-217 cluster induced lipid accumulation in FFA-induced LMH cells. Furthermore, the dual luciferase gene reporter assay and RT-qPCR analysis demonstrated that 3-hydroxyacyl-CoA dehydratase 2, F-box protein 8, and transmembrane 9 superfamily member 3 (TM9SF3) were directly targeted by miR-216a/b and miR-217-5p, respectively, and suppressed in the fatty livers of laying hens. Moreover, overexpression of the miR-216/miR-217 cluster or reduction in TM9SF3 levels led to activation of the proliferator-activated receptor/sterol regulatory-element binding protein (PPAR/SREBP) pathway. Overall, these results demonstrate that the miR-216/miR-217 cluster regulates lipid metabolism in laying hens with FLS, which should prove helpful in the development of new interventional strategies.
Nonlinear Control of a Single Tail Tilt Servomotor Tri-Rotor Ducted VTOL-UAV
Authors: Hu Yanpeng , Guo Jin , Ying Pei , Zeng Guannan , Chen Nanyu
Published: May 2022

This paper explores a novel single tail tilt servomotor tri-rotor ducted vertical takeoff and landing unmanned aerial vehicle system (VTOL-UAV), and proposes a compound control method combining disturbance observer, model predictive control (MPC) and sliding mode nonlinear dynamic inversion (NDI), and realizes the robust tracking control of the VTOL-UAV trajectory under compound disturbance. Specifically, the inner loop adopts nonlinear dynamic inverse which improved by sliding mode to realize the pseudo linearization of the system. The outer loop adopts the model predictive control based on the E-SSPC (State Space Predictive Controller based on the Error model) method, on this basis, the sliding mode disturbance observer based on fast Super-twisting algorithm is introduced into the position loop to observe and compensate the disturbance in real time, which improves the robustness of the outer loop system. Numerical simulation experiments verify the effectiveness and robustness of the control method. Finally, the flight test of the VTOL-UAV is carried out.
Morphological Modulation of Broiler Organs in Response to an Organic Acid–Phytogen Composite in Healthy Broilers
Authors: Zeeshan Muhammad , Zaneb Hafsa , Masood Saima , Ashraf Saima , Khan Imad , Rehman Hafiz Faseeh Ur , Din Salahud , Hayat Khizar
Published: May 2022

In this study, we investigated broiler growth traits, intestinal health and immune aggregates in response to supplementation of Origanum vulgare and Cinnamomum verum, citric acid and malic acid in drinking water at varying levels and durations on broiler. We randomly allocated 140 day-old broilers into four (4) groups, with each group having seven replicates (n = 5). A basal diet and water were provided to all the groups ad libitum in accordance with standard farming practices. Keeping group I as the control, group II was supplemented with 1 mL/3.3 L of water for 1–4 days, 1 mL/4 L from 15–18 days and 1 mL/4 L water from 26–29 days. Group III and IV were supplemented with 1 mL/4 L of water and 1 mL/6 L of water for 35 days, respectively. After the completion of the trial (day 35), two broilers per replicate (14 birds per group) were slaughtered to sample the visceral organs. The results demonstrated that the feed conversion ratio (FCR) and body weight gain improved (p ≤ 0.05) during the fourth and fifth week of the trial in group III in comparison to the control group. Although the small intestine villus height and villus-height-to-crypt-depth ratio improved (p ≤ 0.05) in group III, the muscularis externa thickness of the ileum was highest (p ≤ 0.05) in the control group. Similarly, the lymphatic follicular and nodular area of the immune organs improved (p ≤ 0.05) in group III. In all segments of the small intestine (duodenal, jejunal and ileal), the number of acidic and total goblet cells was highest (p ≤ 0.05) in group II. In conclusion, dietary Origanum vulgare, Cinnamomum verum, citric acid and malic acid at the dose of 1 mL/4 L of drinking water (group III) was the most effective in improving the growth performance, gut development and associated immune components in broilers.
Research on the Time-Dependent Split Delivery Green Vehicle Routing Problem for Fresh Agricultural Products with Multiple Time Windows
Authors: Wu Daqing , Wu Chenxiang
Published: May 2022

Due to the diversity and the different distribution conditions of agricultural products, split delivery plays an important role in the last mile distribution of agricultural products distribution. The time-dependent split delivery green vehicle routing problem with multiple time windows (TDSDGVRPMTW) is studied by considering both economic cost and customer satisfaction. A calculation method for road travel time across time periods was designed. A satisfaction measure function based on a time window and a measure function of the economic cost was employed by considering time-varying vehicle speeds, fuel consumption, carbon emissions and customers’ time windows. The object of the TDSDGVRPMTW model is to minimize the sum of the economic cost and maximize average customer satisfaction. According to the characteristics of the model, a variable neighborhood search combined with a non-dominated sorting genetic algorithm II (VNS-NSGA-II) was designed. Finally, the experimental data show that the proposed approaches effectively reduce total distribution costs and promote energy conservation and customer satisfaction.
Bumblebee Pollination Enhances Yield and Flavor of Tomato in Gobi Desert Greenhouses
Authors: Zhang Hong , Han Chao , Breeze Tom D. , Li Mengdan , Mashilingi Shibonage K. , Hua Jun , Zhang Wenbin , Zhang Xuebin , Zhang Shiwen , An Jiandong
Published: May 2022

Bumblebee pollination is crucial to the production of tomato in protected cultivation. Both tomato yield and flavor play important roles in attracting attentions from growers and consumers. Compared with yield, much less work has been conducted to investigate whether and how pollination methods affect tomato flavor. In this study, the effects of bumblebee pollination, vibrator treatment, and plant growth regulator (PGR) treatment on tomato yield and flavor were tested in Gobi Desert greenhouses. Compared with vibrator or PGR treatments, bumblebee pollinated tomato had higher and more stable fruit set, heavier fruit weight, and more seed. We also found that the seed quantity positively correlated with fruit weight in both bumblebee pollinated, and vibrator treated tomato, but not in PGR treated tomato. Besides enhancing yield, bumblebee pollination improved tomato flavor. Bumblebee pollinated tomato fruits contained more fructose and glucose, but less sucrose, citric acid, and malic acid. Furthermore, the volatile organic compounds of bumblebee pollinated tomato were distinctive with vibrator or PGR treated tomato, and more consumer liking related compounds were identified in bumblebee pollinated tomato. Our findings provide new insights into the contributions of bee pollinator towards improving crop yield and quality, emphasizing the importance of bumblebee for tomato pollination.
The Influence of Seed Characteristics on Seed Dispersal Early Stages by Tibetan Macaques
Authors: Qian Hanrui , Li Wenbo , Li Jinhua
Published: May 2022

There are numerous ecological and evolutionary implications for the ability of frugivores to predate on fruits and consume or disperse their seeds. Tibetan macaques, which are considered important seed predators, typically feed on fruits or seeds. However, systematic research into whether they have a seed dispersal function is still lacking. Endozoochory allows seeds to disperse over greater distances by allowing them to remain in the animal’s digestive tract. Consumption of fruit may not imply effective seed dispersal, and the physical characteristics of seeds (e.g., size, weight, specific gravity, etc.) may influence the dispersal phase’s outcome. We conducted feeding experiments with three captive Tibetan macaques (Macaca thibetana) and nine plant seeds to determine the influence of seed characteristics on Tibetan macaques’ early stages of seed dispersal. The results revealed that the percentage of seed destruction (PSD) after ingestion was 81.45% (range: 15.67–100%), with the PSD varying between plant species. Among the three passage time parameters, the transit time (TT) (mean: 18.8 h and range: 4–24 h) and the time of seed last appearance (TLA) (mean: 100.4 h and range: 48–168 h) differed significantly between seed species, whereas the mean retention time (MRT) (mean: 47.0 h and range: 32–70.3 h) did not. In terms of model selection, PSD was influenced by seed size, weight, volume, and specific gravity; TT was influenced by seed-to-shell investment rate, weight, volume, and specific gravity; and TLA was influenced only by seed size. These findings imply that seeds with a smaller size, specific gravity, volume, and greater weight pass more easily through the monkeys’ digestive tracts. Particularly, seeds with a mean cubic diameter (MCD) of <3 mm had a higher rate of expulsion, larger volume, and weight seeds pass faster, while smaller remained longer. Tibetan macaques, as potential seed dispersers, require specific passage time and passage rates of small or medium-sized seeds. Larger and heavier seeds may be more reliant on endozoochory. Tibetan macaques have the ability to disperse seeds over long distances, allowing for gene flow within the plant community.
Are Local Dairy Products Better? Using Principal Component Analysis to Investigate Consumers’ Perception towards Quality, Sustainability, and Market Availability
Authors: Merlino Valentina Maria , Renna Manuela , Nery Joana , Muresu Arianna , Ricci Alessandro , Maggiolino Aristide , Celano Giuseppe , De Ruggieri Barbara , Tarantola Martina
Published: May 2022

Consumers are increasingly aware of the benefits of local foods in terms of quality, sustainability, animal welfare, and safety. This research addresses two main questions: (i) is the perception towards sustainability aspects of local dairy products related to individuals’ preferences for milk and cheese quality aspects? (ii) are these perceptions related to people’s socio-demographic characteristics? For this purpose, a choice experiment was conducted online in Apulia (South-East Italy) involving a sample of 543 respondents. A Principal Component Analysis (PCA) was applied to analyze the consumer opinion related to dairy products’ quality attributes, sustainability, and availability on the market. From the PCA, four main components (accounting the 64.5% of the total explained variance) were defined, highlighting non-overlapping choice styles of consumers, distinguished by attitudes primarily based on quality attributes of dairy products rather than sustainability characteristics or perceived higher quality of local products. Furthermore, the Availability request component described the consumer need for higher availability and/or visibility of local dairy products on the market. The effect of gender, age, and educational status of individuals emerged as significantly important for the resulting component definitions. The obtained results clearly suggest the need to increase the efficiency of communication strategies concerning local dairy products, as well as local dairy products’ availability and visibility on the markets.
Current State of In Vitro Embryo Production in African Lion (Panthera leo)
Authors: Zahmel Jennifer , Skalborg Simonsen Kim , Stagegaard Julia , Palma-Vera Sergio Eliseo , Jewgenow Katarina
Published: May 2022

In the last 30–40 years, in vitro maturation (IVM) and fertilization (IVF) of domestic cat oocytes have been established as part of the panel of assisted reproduction technologies. As a representative of wild felids, the African lion is not yet considered endangered. Nevertheless, the zoo population management of the African lion itself as well as other closely related felids would benefit from the establishment of an IVF system. Here, we aimed to investigate the transferability of domestic cat IVF technology to the African lion. From the ovaries of 42 lionesses aged between 0.75 and 15 years, a total of 933 IVF-suitable oocytes were retrieved and subjected to IVM and IVF. The overall maturation rate was 40.6% and 18.9% of these oocytes cleaved after fertilization, respectively. Embryos were generated by intracytoplasmic sperm cell injection as well as co-culture with epididymal sperm. Improvements in the model system also led to an improved outcome with in vitro produced embryos in the lion. Compared to domestic cats, the transportation of gonads to a specialized laboratory was time-consuming and influenced oocyte quality negatively. In conclusion, the domestic cat IVF system is adoptable for the African lion, although success rates are still lower.
One-Year Clinical, Microbiological and Immunological Results of Local Doxycycline or Antimicrobial Photodynamic Therapy for Recurrent/Persisting Periodontal Pockets: A Randomized Clinical Trial
Authors: Cosgarea Raluca , Ramseier Christoph A. , Jepsen Søren , Arweiler Nicole Birgit , Jervøe-Storm Pia Merete , Batori-Andronescu Ionela , Rößler Ralf , Conrad Torsten , Eick Sigrun , Sculean Anton
Published: May 2022

We evaluated, in this study, the clinical, microbiological and immunological effects of local drug delivery (LDD) or photodynamic therapy (PDT), adjunctive to subgingival instrumentation (SI) in persistent or recurrent periodontal pockets in patients enrolled in supportive periodontal therapy (SPT) after one year. A total of 105 patients enrolled in SPT with persistent/recurrent pockets were randomly treated with SI +PDT or SI + LDD or SI (control). The number of treated sites with bleeding on probing (n BOP+), probing pocket depths (PPD), clinical attachment level (CAL), full-mouth plaque and bleeding scores (gingival bleeding index, %bleeding on probing-BOP) was evaluated at baseline and after 12 months. Additionally, eight periodontopathogens and the immunomarkers IL-1β (interleukin)and MMP-8 (matrix metalloprotease) were quantitatively determined using real-time PCR and ELISA, respectively. All three treatments resulted in statistically significant clinical improvements (p < 0.05) without statistically significant intergroup differences (p > 0.05), which were maintained up to 12 months. The presence of BOP negatively affected the PPD and CAL. Moreover, statistically significantly fewer bleeding sites at 12 months were observed in the test groups (p = 0.049). Several periodontopathogens were reduced after 12 months. In conclusion, the present data indicate that in periodontal patients enrolled in SPT, treatment of persistent/recurrent pockets with SI alone or combined with either PDT or LDD may lead to comparable clinical, microbiological and immunological improvements, which are maintained up to 12 months. Secondly, the presence of BOP directly impacts the PPD and CAL.
Correction: Nulsopapon et al. The Synergistic Activity and Optimizing Doses of Tigecycline in Combination with Aminoglycosides against Clinical Carbapenem-Resistant Klebsiella pneumoniae Isolates. Antibiotics 2021, 10, 736
Authors: Nulsopapon Parnrada , Nasomsong Worapong , Pongchaidecha Manat , Changpradub Dhitiwat , Juntanawiwat Piraporn , Santimaleeworagun Wichai
Published: May 2022

There were errors made in the original article [...]
Antiamoebic Properties of Laboratory and Clinically Used Drugs against Naegleria fowleri and Balamuthia mandrillaris
Authors: Siddiqui Ruqaiyyah , Mungroo Mohammad Ridwane , Anuar Tengku Shahrul , Alharbi Ahmad M. , Alfahemi Hasan , Elmoselhi Adel B. , Khan Naveed Ahmed
Published: May 2022

Naegleria fowleri and Balamuthia mandrillaris are pathogenic free-living amoebae that infect the central nervous system with over 95% mortality rates. Although several compounds have shown promise in vitro but associated side effects and/or prolonged approval processes for clinical applications have led to limited success. To overcome this, drug repurposing of marketed compounds with known mechanism of action is considered a viable approach that has potential to expedite discovery and application of anti-amoebic compounds. In fact, many of the drugs currently employed in the treatment of N. fowleri and B. mandrillaris, such as amphotericin B, fluconazole, rifampin and miltefosine, are repurposed drugs. Here, we evaluated a range of clinical and laboratory compounds including metformin, quinclorac, indaziflam, inositol, nateglinide, 2,6-DNBT, trans-cinnamic acid, terbuthylazine, acarbose, glimepiride, vildagliptin, cellulase, thaxtomin A, repaglinide and dimethyl peptidase (IV) inhibitor against N. fowleri and B. mandrillaris. Anti-amoebic assays revealed that indaziflam, nateglinide, 2,6-DNBT, terbuthylazine, acarbose and glimepiride exhibited potent amoebicidal properties against both N. fowleri and B. mandrillaris. Notably, all compounds tested showed minimal human (HaCaT) cell cytotoxicity as determined by lactate dehydrogenase release. Prospective research using animal models is warranted to determine the potential of these repurposed compounds, as well as the need for investigating the intranasal route of delivery to treat these devastating infections.
Reasoning Algorithms on Feature Modeling—A Systematic Mapping Study
Authors: Sepúlveda Samuel , Cravero Ania
Published: May 2022

Context: Software product lines (SPLs) have reached a considerable level of adoption in the software industry. The most commonly used models for managing the variability of SPLs are feature models (FMs). The analysis of FMs is an error-prone, tedious task, and it is not feasible to accomplish this task manually with large-scale FMs. In recent years, much effort has been devoted to developing reasoning algorithms for FMs. Aim: To synthesize the evidence on the use of reasoning algorithms for feature modeling. Method: We conducted a systematic mapping study, including six research questions. This study included 66 papers published from 2010 to 2020. Results: We found that most algorithms were used in the domain stage (70%). The most commonly used technologies were transformations (18%). As for the origins of the proposals, they were mainly rooted in academia (76%). The FODA model continued to be the most frequently used representation for feature modeling (70%). A large majority of the papers presented some empirical validation process (90%). Conclusion: We were able to respond to the RQs. The FODA model is consolidated as a reference within SPLs to manage variability. Responses to RQ2 and RQ6 require further review.
High Power All-Fiber Supercontinuum System Based on Graded-Index Multimode Fibers
Authors: Zhang Haitao , Zu Jiaqi , Liu Xiaozheng , Chen Junyu , Xu Haozhen
Published: May 2022

An all-fiber supercontinuum source based on graded-index multimode fibers is reported. The supercontinuum source is based on a homemade mode-locked oscillator and a three-stage picosecond amplifier, which obtained the supercontinuum by a graded-index multimode fiber. The laser output with a spectral range of 480–2440 nm, an average power of 25 W, and a repetition frequency of 8.27 MHz is obtained. To the best of our knowledge, this is the highest average power for generating a supercontinuum with an all-fiber structure based on the graded-index fiber. The effects of GRIN fiber length and different pump peak powers on the supercontinuum generation are also verified. The results showed that the graded-index multimode fiber can effectively obtain a supercontinuum with high power.
Digital Planning Technique for Surgical Guides for Prosthetic Implants before Orthodontic Treatment
Authors: Kirlys Robertas , Nedzinskaitė Rugilė , Rongo Roberto , Severino Marco , Puisys Algirdas , D’Antò Vincenzo
Published: May 2022

Background: The use of digitalization allows clinicians to plan the position of dental implants in orthodontic patients treated with aligners in the beginning of their orthodontic treatment, instead of waiting until the end of orthodontic treatment, integrating implant restorations with orthodontic biomechanics. Aim: The aim of this case report is to describe a digital workflow for aligner treatment supported by implants, in which implants are placed at the beginning of the treatment and support the orthodontic treatment. Materials and Methods: Digital planning for orthodontic treatment with clear aligners and the preparation of surgical guides for implant surgery before orthodontic treatment are used to solve two multidisciplinary cases. Cone-beam computed tomography (CBCT) and virtual dental impressions are used for the virtual planning. Successively, a surgical guide for implant placement and aligners are used in the treatment plan. Results: The digital orthodontic setup with the integration of the root position allows the clinician to plan the position of dental implants on the final occlusion. The placement of the implant before the start of the orthodontic treatment allows the implants to be used as skeletal anchorage, thereby helping the orthodontic biomechanics. Conclusions: This study describes how to perform a digital workflow with orthodontic virtual planning and the design of surgical guides for implant placement. This technique is potentially promising for complex orthodontic cases.
Episodic Memory and Information Recognition Using Solitonic Neural Networks Based on Photorefractive Plasticity
Authors: Bile Alessandro , Tari Hamed , Fazio Eugenio
Published: May 2022

Neuromorphic models are proving capable of performing complex machine learning tasks, overcoming the structural limitations imposed by software algorithms and electronic architectures. Recently, both supervised and unsupervised learnings were obtained in photonic neurons by means of spatial-soliton-waveguide X-junctions. This paper investigates the behavior of networks based on these solitonic neurons, which are capable of performing complex tasks such as bit-to-bit information memorization and recognition. By exploiting photorefractive nonlinearity as if it were a biological neuroplasticity, the network modifies and adapts to the incoming signals, memorizing and recognizing them (photorefractive plasticity). The information processing and storage result in a plastic modification of the network interconnections. Theoretical description and numerical simulation of solitonic networks are reported and applied to the processing of 4-bit information.
Mixed-Integer-Based Path and Morphing Planning for a Tensegrity Drone
Authors: Savin Sergei , Al Badr Amer , Devitt Dmitry , Fedorenko Roman , Klimchik Alexandr
Published: May 2022

This paper proposes a method for simultaneously planning a path and a sequence of deformations for a tensegrity drone. Previous work in the field required the use of bounding surfaces, making the planning more conservative. The proposed method takes advantage of the need to use mixed-integer variables in choosing the drone path (using big-M relaxation) to simultaneously choose the configuration of the drone, eliminating the need to use semidefinite matrices to encode configurations, as was done previously. The numerical properties of the algorithm are demonstrated in numerical studies. To show the viability of tensegrity drones, the first tensegrity quadrotor Tensodrone was build. The Tensodrone is based on a six-bar tensegrity structure that is inherently compliant and can withstand crash landings and frontal collisions with obstacles. This makes the robot safe for the humans around it and protects the drone itself during aggressive maneuvers in constrained and cluttered environments, a feature that is becoming increasingly important for challenging applications that include cave exploration and indoor disaster response.
Peri-Implantitis: A New Definition Proposal Based on Unnatural Spatial Arrangement and Late Mechanical Coupling between Two Cortical Bone Layers during Osseointegration Phase Part II
Authors: Ihde Stefan , Ihde Antonina , Sipic Olga , Pałka Łukasz
Published: May 2022

To date, the term peri-implantitis has been mostly associated with bacterial or foreign body reaction as primary factors of its development. Because of this, researchers’ and clinicians’ attention regarding treatment possibilities were directed into the solutions on the basis of surface modifications, debridement, and antibiotics. After years of clinical observations and poor results in treatment of peri-implantitis, a new proposal of this condition is presented, shifting our way of thinking regarding bone and implant interactions. In the second part of the paper presenting a new definition of peri-implantitis, we focused on a biological explanation of the bone behavior at the bone–implant interface. The main conclusion is that PI is not an “infectious disease”, but rather the result of natural changes of the bone’s morphology in response to implant such as a decrease in convexity of the outer surface of the bone and subsequently a decrease in concavity of the inner bone.
Optomechanical Analysis and Design of Polygon Mirror-Based Laser Scanners
Authors: Duma Virgil-Florin , Duma Maria-Alexandra
Published: May 2022

Polygon Mirror (PM)-based scanning heads are one of the fastest and most versatile optomechanical laser scanners. The aim of this work is to develop a multi-parameter opto-mechanical analysis of PMs, from which to extract rules-of-thumbs for the design of such systems. The characteristic functions and parameters of PMs scanning heads are deduced and studied, considering their constructive and functional parameters. Optical aspects related to the kinematics of emergent laser beams (and of corresponding laser spots on a scanned plane or objective lens) are investigated. The PM analysis (which implies a larger number of parameters) is confronted with the corresponding, but less complex aspects of Galvanometer Scanners (GSs). The issue of the non-linearity of the scanning functions of both PMs and GSs (and, consequently, of their variable scanning velocities) is approached, as well as characteristic angles, the angular and linear Field-of-View (FOV), and the duty cycle. A device with two supplemental mirrors is proposed and designed to increase the distance between the GS or PM and the scanned plane or lens to linearize the scanning function (and thus to achieve an approximately constant scanning velocity). These optical aspects are completed with Finite Element Analyses (FEA) of fast rotational PMs, to assess their structural integrity issues. The study is concluded with an optomechanical design scheme of PM-based scanning heads, which unites optical and mechanical aspects—to allow for a more comprehensive approach of possible issues of such scanners. Such a scheme can be applied to other types of optomechanical scanners, with mirrors or refractive elements, as well.