bluuebunny/crossref_metadata_2025 huggingface dataset:
Video Promosi Gerai Must Break Coffee & Eatery Untuk Meningkatkan Loyalitas Konsumen
Authors: Faisal Muhammad , Utami Wiranti Sri , Putra Prakoso Wahyu Aji
Published: March 2023

The strategy commonly used in the business world is in the form of media promotion, this is done to introduce a service to the wider community so that a sense of interest in offering services or products is created. This promotional activity was also carried out by the Must Break Coffee coffee shop, activities carried out in promoting coffee drinks at Must Break Coffee previously used brochures, banners and social media. Must Break Coffee is a business entity that sells coffee drinks as their main menu. The problem faced at this time is the lack of forms of promotion carried out by Must Break Coffee, therefore a new form of promotion is needed that can make Must Break Coffee better known by the public. The purpose of this study is to provide more complete information about the coffee products sold by Must Break Coffee in the form of the Must Break Coffee logo, the services available at Must Break Coffee, the facilities available at Must Break Coffee, and others. The method used in this study is to collect data, analyze using SWOT techniques, media design and media production concepts (KPM) which include pre-production, production and post-production using computer software, namely Adobe Premiere. This promotional video will be implemented through social media.

Keywords—Promotion Video, Coffee Shop, Strategi

 

Strategi yang biasa digunakan dalam dunia bisnis adalah dalam bentuk media promosi hal ini dilakukan untuk memperkenalkan suatu layanan kepada masyarakat luas agar terciptanya rasa ketertarikan dalam menawarkan jasa ataupun produk. Kegiatan promosi ini juga dilakukan oleh coffee shop Must Break Coffee, kegiatan yang dilakukan dalam mempromosikan minuman coffee pada Must Break Coffee sebelumnya menggunakan media Brosur, Banner, dan Sosial Media. Must Break Coffee merupakan suatu badan usaha yang menjual minuman coffee sebagai menu utama mereka. Permasalahan yang dihadapi saat ini adalah masih minimnya bentuk promosi yang dilakukan oleh Must Break Coffee, oleh karena itu dibutuhkan suatu bentuk promosi baru yang dapat membuat Must Break Coffee lebih dikenal oleh masyarakat. Tujuan dari penelitian ini adalah untuk memberikan informasi yang lebih lengkap mengenai produk kopi yang di jual oleh Must Break Coffee hal tersebut berupa logo Must Break Coffee, layanan yang ada pada Must Break Coffee, Fasilitas yang ada pada Must Break Coffee, dan lain-lain. Metode yang digunakan pada penelitian ini adalah dengan cara mengumpulkan data, Analisa menggunakan teknik SWOT, Perancangan Media dan Konsep Produksi Media (KPM) dimana termasuk dari pre production, production, dan post production dengan menggunakan perangkat lunak computer yaitu Adobe Premiere. Video promosi ini akan di implementasikan melalu media social.

Kata Kunci—Video Promosi, Coffee Shop, Strategi
Application of Naive Bayes Model, SVM and Deep Learning Predicting
Authors: Padeli Padeli , Martono Aris , Sudaryono Sudaryono
Published: March 2023

The college hopes that every semester students are able to pay tuition properly and smoothly. The hope is that the institution will be able to  maintain  monthly  cash  flow so  that  its  operational  and maintenance costs can be met. Therefore, this study was conducted to predict and fulfill the institution's cash-in from the method of paying tuition fees either by cash, installments, or sometimes late payments every semester. In predicting the method of paying tuition fees, using student  profile data (name,  name,  study program)  and achievement index  every  semester  for  5  semesters  passed  and  the  method  of payment  (cash,  installments,  and  late--cash  or  installments).  Using the Naive Bayes (NB) method, Support Vector Machine (SVM), and Deep Learning, this study aims to forecast tuition costs. The Classification Prediction Model with Naive Bayes, SVM, and Deep Learning produces Confusion Matrix Performance NB with an Accuracy of 91.49%, Confusion Matrix Performance SVM with an Accuracy of 85.11%, and Confusion Matrix Performance Deep Learning with an Accuracy of 89.36%, according to the research findings.

Keywords—Payments, Algorithm, Performance
Rancang Bangun Sistem Pemilahan dan Pengangkutan Sampah Wilayah Provinsi DKI Jakarta Berbasis Website Dengan Metode Prototype
Authors: Rahman Ardi Dwi , Setiawati Popong
Published: March 2023

Data pemilahan dan pengangkutan sampah terjadwal pada Dinas Lingkungan Hidup Provinsi DKI Jakarta masih menggunakan buku catatan/manual dan menggunakan Google From. Hal ini mempersulit bagi pimpinan/kepala Dinas Lingkungan Hidup Provinsi DKIJakarta untuk memonitoring seluruh data laporan pemilahan dan pengangkutan sampah terjadwal di wilayah DKI Jakarta. Penelitian ini  mengembangkan sebuah aplikasi dari permasalahan tersebutyakni pemilahan dan pengangkutan sampah terjadwal berbasis web sehingga meringankan Dinas Lingkungan Hidup dalam pengelolaan data dan memonitoring pemilahan dan pengangkutan sampah terjadwal. Aplikasi ini dibuat menggunakan modeling diagram UML (Unified Modeling Language) sedangkan Pemrograman PHP Bootstrap dan database MySQL. Metode pengumpulan data ialah wawancara, observasi, studi dokumentasi serta kepustakaan, selanjutnya softwarey ang digunakan metode protoype. Dengan dibangunnya system ini diharapkan pemilahan sampah mulai sumber menjadi begitu penting dilakukan guna meningkatkan persentase daur ulang sampah serta mengurangi jumlah sampah yang berakhir di Tempat Pembuangan Akhir.
Penerapan Mikrokontroler pada Penutup Tempat Sampah untuk Membuka atau Menutup secara Otomatis ( Studi Kasus di Universitas Raharja)
Authors: Martono Aris , Yulianjani Arsi , Sudariyanti Amilia
Published: March 2023

Penelitian ini bertujuan pembuatan prototipe penutup tempat sampah secara otomatis. Penutup tempat sampah otomatis ini menggunakan rancangan perakitan mikrokontroler Arduino Uno ATMega328 agar menyadarkan masyarakat tentang pentingnya kesehatan melalui pembuangan sampah. Metode yang digunakan untuk mendeteksi keberadaan objek ini adalah metode desain yang terdiri dari beberapa langkah yaitu (1) analisis kebutuhan, (2) desain, (3) implementasi rangkaian. Prototipe ini terbagi menjadi dua bagian yaitu perangkat keras dan perangkat lunak. Perangkat keras terdiri dari sensor ultrasonik HC-SR04, sensor PIR, sistem mikrokontroler ATMega328 minimal dengan satu set pengontrol input dan output dan motor servo, sedangkan perangkat lunak dibangun dengan Arduino, yang mirip dengan bahasa pemrograman C (Arduino). Hasil pembacaan data jarak sensor ultrasonik kemampuan mendeteksi objek dari tempat sampah sejauh 25cm dan waktu menutup tempat sampah sekitar 5 detik. Penggunaan sensor PIR untuk mendeteksi keberadaan Orang. Berdasarkan jarak yang telah ditentukan, ketika objek yang mendekat dalam jarak 25 cm terdeteksi, komputer mikro mengontrol motor servo untuk membuka dan menutup tempat sampah secara otomatis. Dengan menggunakan mikrokontroler ATMega328, fungsi tempat sampah otomatis banyak dibutuhkan untuk menjaga kebersihan dan kesehatan lingkungan.
Analisis Ruang Virtual Menggunakan Augmented Reality Untuk Meningkatkan Promosi Penjualan Bagi UMKM
Authors: Warsito Ary Budi , Yusup Muhammad , Nugroho Wijil
Published: March 2023

Digital marketing adalah upaya pemasaran produk dengan media digital dan jaringan internet. Seperti Google Bisnisku, WhatsApp Bisnis, website, dan platform lainnya. Faktanya, pengguna internet di dunia sudah 4,8 miliar orang lebih (Data Reportal). Itu artinya, makin banyak orang terhubung dengan internet sehingga upaya digital marketing mulai diperlukan. Melihat peluang emas itu, berbagai tingkatan bisnis pun mengadopsi strategi digital marketing. Sehingga, mereka bisa menjangkau target pasar yang lebih luas. Mengapa perlu digital marketing, hal ini dikarenakan digital marketing memiliki manfaat diantaranya adalah fleksibel digunakan semua jenis bisnis, mampu menjangkau konsumen secara personal, lebihmemudahkan dalam mengukur keberhasilan strategis dan memaksimalkan tingkat konversi karena mampu melemparkan ke target audiens spesifik. Oleh karena itu perlu diterapkan digital marketing pada pelaku usaha mikro, kecil dan menengah agar kegiatan usaha mereka dapat tetap berjalan, Berdasarkan data Kementerian Koperasi dan Usaha Kecil dan Menengah (Kemenkop UKM) bulan Maret 2021, jumlah UMKM mencapai 64,2 juta yang tersebar di seluruh indonesia. Dari data tersebut pelaku UMKM masih banyak yang belum atau mampu memanfaat media digital. Ruang virtual merupakan solusi yang baik untuk meningkatkan brand produk, hal ini didasarkankan ada interaksi antara produk dengan konsumen tanpa harus berhubungan langsung dengan produsen, konsepnya adalah pada saat konsumen memindai gawai ke produk maka produk tersebut akan muncul sebuah informasi tentang produk tersebut. Penelitian tahap awal ini berlangsung selama kurang lebih enam bulan dimana keluaran yang diharapkan adalah sebuah aplikasi virtual yang dapat digunakan oleh para pelaku usaha UMKM di indonesia untuk mempercepat menginformasikan dan kejelasan produk. karena luasnya wilayah indonesia maka penelitian ini akan difokuskan pada usaha mikro atau kecil yang ada di wilayah kabupaten Tangerang provinsi banten yang dekat dengan tempat peneliti. Sample data yang akan menjadi observasi adalah UMKM Kabupaten Tangerang dimana penelitian yang difokuskan pada usaha mikro dan kecil. Aplikasi yang akan dikembangkan ini menggunakan media website agar pemanfaat bisa menjangkau lebih luas di Indonesia. Pemanfaat aplikasi yang dikembangkan dapat diterapkan pada beberapa pelaku UMKM, mata kuliah digital marketing, mata pelajaran bisnis sekolah bahkan bisa lebih luas lagi. Aplikasi yang dikembangkan ini dapat memungkinkan pelaku usaha memudahkan dalam membuat produk berbasis virtual.
In Covers Green and Gold: Fin-de-siècle Evelina
Authors: Kochkina Svetlana
Published: March 2023

The first novel by Frances Burney, Evelina, Or, a Young Lady’s Entrance into the World, published in 1778, has enjoyed long-standing popularity, having never gone out of print in English. A distinct cluster of its editions appeared at the end of the 19th - beginning of the 20th century. This period in book history was marked by the creative work of private-press book designers, printers, binders, and illustrators inspired by the Aestheticism, Arts and Crafts, and Art Nouveau movements. Even though their pioneering work was destined for narrow circles of collectors and connoisseurs, it profoundly influenced the development of both industrial and artists’ book design, heightening the awareness of books’ aesthetic appeal. The fin de siècle was a time of nostalgia for idealized pre-industrial society, symptomatic of Victorian and Edwardian escapism, which found its expression in lavish book ornaments and illustrations executed in the Directoire and Louis XVI styles. Late 19th - early 20th-century Evelinas, illustrated and decorated by Aubrey Beardsley, Arthur Rackham, and Hugh Thomson, produced by Dent, Newnes, and Macmillan, reflect a profound influence that innovative private presses had on general publishing practices and the quality of industrially produced books. These Evelinas, beautifully illustrated, bound in decorative covers, and well-printed, exemplify both the newly awakened interest in the book’s physical make-up and aesthetical aspects and the late-Victorian and Edwardian escapist nostalgia characteristic of fin de siècle publishing.
The Personal, the Political, And The Psychotherapeutic
Authors: Clulow Christopher
Published: March 2023

It is unthinkable that the turbulence of recent global events will leave couple and family relationships unaffected. Yet the focus of therapists is unlikely to extend much beyond family relationships in understanding and working with the problems of those seeking their help. Is this limited focus justifiable? Can an understanding of psychological processes in couple relationships, especially those around oppression, inform an understanding of political conflict? What implications might there be for psychotherapists? These questions arise from considering intersections between private and public domains of life in four contexts: the decision of the United Kingdom to leave the European Union; the global pandemic of Covid-19; the war in Ukraine; and the climate emergency.
Facing a New Reality: Psychotherapy Against The Background of War
Authors: Konina Mira
Published: March 2023

The saying that "in order to leave the comfort zone, you must first enter one" is especially relevant against a background of external turbulence. The search for this zone brings people to psychotherapy, especially when early and current trauma intersect. Patients learn how to live in a new reality, enhance old supports, and discover new ones within themselves and in those relationships they perceive as secure. This article describes clinical work unfolding against the backdrop of the Russian–Ukrainian conflict. I outline the development of the relationship between a Ukrainian emigrant, Maria, and her French partner, Eduard, both traumatised by childhood events and living in their own "illusory" worlds where it was impossible for them to meet their true selves and each other. As a result of keeping the couple in mind in Maria's individual psychotherapeutic work, and under the influence of the events taking place in the world, they go through significant intrapersonal changes that allow them to fill their lives and relationships with new meaning.
Symptoms of Normality: Boundaries, Identity, And Symbolisation
Authors: Monguzzi Fabio
Published: March 2023

A significant part of psychic suffering is connected to the contemporary sociocultural crisis. The impressive transformations that have taken place in recent decades have led to a different and more complex organisation of needs and of the social and emotional life. On the psychic level, a diminished capacity for symbolic representation and a profound disorientation in processes of subjectivation have been observed. Couple crises frequently reveal the fragility of the link in its constituent aspects where individuals appear to have difficulty in forming intersubjective contracts. The function of the couple psychotherapist becomes essential in rooting the relationship within a more meaningful and profound psychic dimension.
How Far Do Current Theories and Techniques Go Towards Helping the Families and Couples of Today?
Authors: Scharff David E.
Published: March 2023

The author reviews his own history of applying psychoanalysis to the understanding and treatment of families and couples over a career of more than fifty years to ask the question: what elements of analytic understanding and practice still apply to the changing forms of families in the world and the complexity of situations in which families find themselves? He also considers the recent changes brought about by the Covid-19 pandemic and by deteriorating geopolitical situations. He concludes that analytic understanding of the unconscious organisation of the individual and the shared unconscious of the family, and the consideration of historical and cultural contexts, continues to offer a rich set of orienting ideas for our work.
Evolution of fetal ventriculomegaly diagnosed in the second trimester of pregnancy: A retrospective study in Northern Greece
Authors: Arampatzopoulou Maria-Elisavet , Tsakiridis Ioannis , Dagklis Themistoklis , Mamopoulos Apostolos , Athanasiadis Apostolos
Published: March 2023

Introduction: The present retrospective study aims to examine the course of ventriculomegaly (VM) during gestation, the association between the degree of VM and the presence of additional sonographic fetal malformations, as well as to inspect the prevalence of VM with reference to fetal gender. Materials and Methods: The databases of two maternal-fetal ultrasound units were reviewed from 2010 to 2021. All cases were classified as either mild (10 to <12mm), moderate (12 to <15mm), or severe (≥15mm) according to the fetal posterior ventricle width measurement upon VM diagnosis, at 20-24 weeks of gestation. Furthermore, cases with additional fetal sonographic abnormalities were registered as cases of non-isolated VM, whereas those cases without further fetal malformations on ultrasound (US) scan were documented as cases of isolated VM. The final sonographic record of every participant, following VM diagnosis, was registered so that the evolution of VM during gestation could be monitored. Results: The sonographic and medical records of 81 women diagnosed with fetal VM were studied. The prevalence of VM is about 0.24% and the male/female ratio was 2; 88.9% of the cases were mild, 9.9% moderate, while only 1 (1.2%) was severe at diagnosis. Among the initial 62 cases of mild VM, 24 (38.7%) regressed, 16 (25.8%) remained unchanged and 22 (35.5%) progressed. There was an association between a higher degree of VM and the presence of additional fetal abnormalities. Conclusions: The majority of VM cases are mild at diagnosis and in isolated about one third resolves in the third trimester and about one third progresses but not to severe. Therefore, a follow up scan is useful to reassure most parents and identify those cases that may progress to moderate.
Asymptomatic bacteriuria in pregnant Nigerian women at the Lagos University Teaching Hospital, south west Nigeria
Authors: Oluwole Ayodeji A. , Ugwu Aloy Okechukwu , Omisakin Sunday I. , Akinajo Rebecca Opeyemi
Published: March 2023

Background: Dipstick urinalysis is a handy and cost effective method for the early detection of asymptomatic bacteriuria. Asymptomatic bacteriuria occurs in 2-10% of pregnancies and has been associated with low birth weight and preterm delivery in pregnant women if untreated. Objective: To determine the incidence of asymptomatic bacteriuria in pregnancy, the distribution of infecting organisms and the antibiotic sensitivity pattern in pregnant Nigerian women attending the antenatal clinic of the Lagos University Teaching Hospital, Idi- Araba, Lagos. Methodology: This was a prospective study. The study population were 201 consecutive asymptomatic pregnant women, who attended the antenatal clinic of LUTH for booking between 1st July and 31st December, 2021. Two consecutive urine samples were collected and analysed for microscopy, culture and sensitivity and urinalysis. The outcomes of interest were prevalence of asymptomatic bacteriuria at LUTH, bacterial isolates, and their antibiotic sensitivity pattern. Results: The mean age of the population studied was 31years ± 4.56SD. The mean gestational age was 23.8 ± 5.39 SD with a range of 11-34 weeks; and majority of the participants (65.7%) were recruited at gestational ages between 14 and 26 weeks. The prevalence of asymptomatic bacteriuria in this study was 13.9%. The commonest isolated organism was klebsiella (46.4%) followed by Escherichia coli (21.4%). Ceftazidime, ofloxacin, and gentamycin showed good overall sensitivity with 92.9%, 89.3%, and 67.9%% respectively. From the results of urinalysis, nitrites showed a sensitivity of 48.3% and specificity of 98.2% with a positive predictive value of 83.3% while presence of bacterial cells on urine microscopy showed sensitivity of 31.2%, specificity of 85% and a positive predictive value of 27%. Conclusion: The incidence of asymptomatic bacteriuria in this study was considerably high and therefore, there may be need to consider introduction of routine urine culture at booking or in the second trimester.
On Techno-Tantrik Embodiment
Authors: Bedos Clémentine
Published: March 2023

Inspired by Paul B. Preciado’s work of autotheory, Testo Junkie, and the various processes of self-shaping that circulate through his corpus, this visual piece and its accompanying personal essay draw upon the artist’s familial and ancestral knowledge of their grandmother’s confinement in an asylum. Developing a visual methodology based on the holographic philosophy of Nondual Śaiva Tantra, Techno-Tantrik Embodiment seeks to offer analogous holistic technologies that harness the power of the repressed and the taboo, to transform mind, body and environment. The piece questions how technological scale sets conditions for relations, feelings, democratic processes and infrastructures, and it provides a mixed-reality approach to consciousness, asking: what types of engagement with image-based systems can subvert and transform our worldview? Utilizing a queer framework that eschews respectability or a quest for ‘positive’ counternarratives, it explores the possible synergies between Tantrik and digital technologies, to build meaningful connections with ourselves and alterity.
Ancestry dependent balancing selection of placental dysferlin at high-altitude
Authors: Gundling William E. , Post Sasha , Illsley Nicholas P. , Echalar Lourdes , Zamudio Stacy , Wildman Derek E.
Published: March 2023

Introduction: The placenta mediates fetal growth by regulating gas and nutrient exchange between the mother and the fetus. The cell type in the placenta where this nutrient exchange occurs is called the syncytiotrophoblast, which is the barrier between the fetal and maternal blood. Residence at high-altitude is strongly associated with reduced 3rd trimester fetal growth and increased rates of complications such as preeclampsia. We asked whether altitude and/or ancestry-related placental gene expression contributes to differential fetal growth under high-altitude conditions, as native populations have greater fetal growth than migrants to high-altitude.

Methods: We have previously shown that methylation differences largely accounted for altitude-associated differences in placental gene expression that favor improved fetal growth among high-altitude natives. We tested for differences in DNA methylation between Andean and European placental samples from Bolivia [La Paz (∼3,600 m) and Santa Cruz, Bolivia (∼400 m)]. One group of genes showing significant altitude-related differences are those involved in cell fusion and membrane repair in the syncytiotrophoblast. Dysferlin (DYSF) shows greater expression levels in high- vs. low-altitude placentas, regardless of ancestry. DYSF has a single nucleotide variant (rs10166384;G/A) located at a methylation site that can potentially stimulate or repress DYSF expression. Following up with individual DNA genotyping in an expanded sample size, we observed three classes of DNA methylation that corresponded to individual genotypes of rs10166384 (A/A < A/G < G/G). We tested whether these genotypes are under Darwinian selection pressure by sequencing a ∼2.5 kb fragment including the DYSF variants from 96 Bolivian samples and compared them to data from the 1000 genomes project.

Results: We found that balancing selection (Tajima’s D = 2.37) was acting on this fragment among Andeans regardless of altitude, and in Europeans at high-altitude (Tajima’s D = 1.85).

Discussion: This supports that balancing selection acting on dysferlin is capable of altering DNA methylation patterns based on environmental exposure to high-altitude hypoxia. This finding is analogous to balancing selection seen frequency-dependent selection, implying both alleles are advantageous in different ways depending on environmental circumstances. Preservation of the adenine (A) and guanine (G) alleles may therefore aid both Andeans and Europeans in an altitude dependent fashion.
Construction and evaluation of DNA vaccine encoding Crimean Congo hemorrhagic fever virus nucleocapsid protein, glycoprotein N-terminal and C-terminal fused with LAMP1
Authors: Hu Yong-Liang , Zhang Lian-Qing , Liu Xiao-Qian , Ye Wei , Zhao Yue-Xi , Zhang Liang , Qiang Zun-Xian , Zhang Lin-Xuan , Lei Ying-Feng , Jiang Dong-Bo , Cheng Lin-Feng , Zhang Fang-Lin
Published: March 2023

Crimean-Congo hemorrhagic fever virus (CCHFV) can cause severe hemorrhagic fever in humans and is mainly transmitted by ticks. There is no effective vaccine for Crimean-Congo hemorrhagic fever (CCHF) at present. We developed three DNA vaccines encoding CCHFV nucleocapsid protein (NP), glycoprotein N-terminal (Gn) and C-terminal (Gc) fused with lysosome-associated membrane protein 1 (LAMP1) and assessed their immunogenicity and protective efficacy in a human MHC (HLA-A11/DR1) transgenic mouse model. The mice that were vaccinated three times with pVAX-LAMP1-CCHFV-NP induced balanced Th1 and Th2 responses and could most effectively protect mice from CCHFV transcription and entry-competent virus-like particles (tecVLPs) infection. The mice vaccinated with pVAX-LAMP1-CCHFV-Gc mainly elicited specific anti-Gc and neutralizing antibodies and provided a certain protection from CCHFV tecVLPs infection, but the protective efficacy was less than that of pVAX-LAMP1-CCHFV-NP. The mice vaccinated with pVAX-LAMP1-CCHFV-Gn only elicited specific anti-Gn antibodies and could not provide sufficient protection from CCHFV tecVLPs infection. These results suggest that pVAX-LAMP1-CCHFV-NP would be a potential and powerful candidate vaccine for CCHFV.
Interactions between antifungals and everolimus against Cryptococcus neoformans
Authors: Liang Pin , Song Jiquan , Liu Qin
Published: March 2023

Cryptococcus is the causal agent of cryptococcosis, a disease with high mortality mainly related to HIV immunosuppression and usually manifests with pneumonia and/or meningoencephalitis. There are very few therapeutic options; thus, innovative approaches are required. Herein, We examined the interaction of everolimus (EVL) with amphotericin B (AmB) and azoles [fluconazole (FLU), posaconazole (POS), voriconazole (VOR), itraconazole (ITR)] against Cryptococcus. Eighteen Cryptococcus neoforman clinical isolates were analyzed. Following the guidelines of the Clinical and Laboratory Standards Institute (CLSI) M27-A4, we conducted a broth microdilution experiment to determine the minimum inhibitory concentrations (MICs) of azoles, EVL, and AmB for assessing antifungal susceptibility. A fractional inhibitory concentration index (FICI) of less than and equal to 0.5 indicated synergy, with a range of 0.5 to 4.0 indicated indifference and a value more than 4.0 indicated antagonism. These experiments revealed that EVL had antifungal activity against C. neoforman. Moreover, EVL, POS, AmB, FLU, ITR, and VOR exhibited MIC values ranging from 0.5-2 μg/mL, 0.03125-2 μg/mL, 0.25-4 μg/mL, 0.5-32μg/mL, 0.0625-4μg/mL and 0.03125-2μg/mL, respectively. The combination of EVL with AmB and azoles (POS, FLU, ITR, and VOR) exhibited synergistic antifungal effects against 16 (88.9%), 9 (50%), 11 (61.1%), 10 (55.6%) or 6 (33.3%) of analyzed Cryptococcus strains. In the presence of EVL, the MIC values of AmB and azoles were significantly lowered. No antagonism was observed. Subsequently, in vivo analyses conducted using the G. mellonella model further confirmed that combination EVL+ POS, EVL+ FLU, and EVL+ITR treatment were associated with significantly improved larval survival following Cryptococcus spp. infection. These findings provide the first published evidence suggesting that a combination of EVL and AmB or azoles exhibit a synergistic effect and may be an effective antifungal disease treatment strategy for infections caused by Cryptococcus spp.
Emergence and circulation of azole-resistant C. albicans, C. auris and C. parapsilosis bloodstream isolates carrying Y132F, K143R or T220L Erg11p substitutions in Colombia
Authors: Ceballos-Garzon Andres , Peñuela Ana , Valderrama-Beltrán Sandra , Vargas-Casanova Yerly , Ariza Beatriz , Parra-Giraldo Claudia M.
Published: March 2023

Methods

Over a four-year period, 123 Candida bloodstream isolates were collected at a quaternary care hospital. The isolates were identified by MALDI-TOF MS and their fluconazole (FLC) susceptibility patterns were assessed according to CLSI guidelines. Subsequently, sequencing of ERG11, TAC1 or MRR1, and efflux pump activity were performed for resistant isolates.

Results

Out of 123 clinical strains,C. albicans accounted for 37.4%, followed by C. tropicalis 26.8%, C. parapsilosis 19.5%, C. auris 8.1%, C. glabrata 4.1%, C. krusei 2.4% and C. lusitaniae 1.6%. Resistance to FLC reached 18%; in addition, a high proportion of isolates were cross-resistant to voriconazole. Erg11 amino acid substitutions associated with FLC-resistance (Y132F, K143R, or T220L) were found in 11/19 (58%) of FLCresistant isolates. Furthermore, novel mutations were found in all genes evaluated. Regarding efflux pumps, 8/19 (42%) of FLC-resistant Candida spp strains showed significant efflux activity. Finally, 6/19 (31%) of FLC-resistant isolates neither harbored resistance-associated mutations nor showed efflux pump activity. Among FLC-resistant species, C. auris 7/10 (70%) and C. parapsilosis 6/24 (25%) displayed the highest percentages of resistance (C. albicans 6/46, 13%).

Discussion

Overall, 68% of FLC-resistant isolates exhibited a mechanism that could explain their phenotype (e.g. mutations, efflux pump activity, or both). We provide evidence that isolates from patients admitted to a Colombian hospital harbor amino acid substitutions related to resistance to one of the most commonly used molecules in the hospital setting, with Y132F being the most frequently detected.
Translating the complexity of disaster resilience with local leaders
Authors: Wowk Kateryna , Adams Margaret , Martinez Emily
Published: March 2023

Disaster resilience involves a complex web of processes, policies, regulatory requirements, and data that is difficult—if not impossible—to fully comprehend, even by seasoned experts. Yet resilience is not owned by “experts”—it is owned by local communities. Local leaders must be empowered to understand and trained to skillfully navigate complex systems to strengthen and continually build their resilience. Similarly, though billions of dollars in federal disaster recovery and mitigation funds are available to help local communities strengthen their resilience, additional work is needed to communicate, understand, assess, and address vulnerabilities at the local level, and particularly in small towns and in underserved communities, by harnessing local knowledge and data. In this article we present a framework developed to guide locally-owned resilience in Texas following Hurricane Harvey. The case study discusses methodologies to enhance the granularity of existing tools that assess resilience and social vulnerability by focusing on the local context for each, while building institutional to individual leadership needed to build disaster resilience over the longer-term. In particular, we discuss approaches being advanced by the Regional Resilience Partnership, which was formed to strengthen capacity for resilience in the 11 county Coastal Bend region, where Harvey first made landfall.
The intervention seasons of thoracic endovascular aortic repair impacted the outcomes for patients with type B aortic dissection
Authors: Zhao Kaiwen , Zhu Hongqiao , He Xiaomin , Liang Taiping , Sun Yudong , Zhou Jian , Jing Zaiping
Published: March 2023

Purpose

The objective of this research was to investigate whether seasonal variations influence the outcomes of type B aortic dissection (TBAD) patients with thoracic endovascular aortic repair (TEVAR).

Patients and methods

From 2003 to 2020, a retrospective cohort study was performed, which included 1,123 TBAD patients who received TEVAR. Medical records were used to gather data on baseline characteristics. Outcomes including all-cause mortality and aortic-related adverse events (ARAEs) were tracked and analyzed.

Results

Of the 1,123 TBAD patients in this study, 308 received TEVAR in spring (27.4%), 240 cases in summer (21.4%), 260 cases in autumn (23.2%), and 315 cases in winter (28.0%). Patients in the autumn group had a significantly lower risk of 1-year mortality than those in the spring group (hazard ratio: 2.66, 95% confidence interval: 1.06–6.67, p = 0.037). Kaplan–Meier curves revealed that patients who underwent TEVAR in autumn had a lower risk of 30-day ARAEs (p = 0.049) and 1-year mortality (p = 0.03) than those in spring.

Conclusion

This study confirmed that TEVAR operated in autumn for TBAD was associated with a lower risk of 30-day ARAEs and 1-year mortality than in spring.
Impact of dobutamine stress on diastolic energetic efficiency of healthy left ventricle: an in vivo kinetic energy analysis
Authors: Riva Alessandra , Eriksson Jonatan , Viola Federica , Sturla Francesco , Votta Emiliano , Ebbers Tino , Carlhäll Carl-Johan Gustav , Dyverfeldt Petter
Published: March 2023

The total kinetic energy (KE) of blood can be decomposed into mean KE (MKE) and turbulent KE (TKE), which are associated with the phase-averaged fluid velocity field and the instantaneous velocity fluctuations, respectively. The aim of this study was to explore the effects of pharmacologically induced stress on MKE and TKE in the left ventricle (LV) in a cohort of healthy volunteers. 4D Flow MRI data were acquired in eleven subjects at rest and after dobutamine infusion, at a heart rate that was ∼60% higher than the one in rest conditions. MKE and TKE were computed as volume integrals over the whole LV and as data mapped to functional LV flow components, i.e., direct flow, retained inflow, delayed ejection flow and residual volume. Diastolic MKE and TKE increased under stress, in particular at peak early filling and peak atrial contraction. Augmented LV inotropy and cardiac frequency also caused an increase in direct flow and retained inflow MKE and TKE. However, the TKE/KE ratio remained comparable between rest and stress conditions, suggesting that LV intracavitary fluid dynamics can adapt to stress conditions without altering the TKE to KE balance of the normal left ventricle at rest.
Building a successful minimally invasive mitral valve repair program before introducing the robotic approach: The Massachusetts General Hospital experience
Authors: van Kampen Antonia , Goudot Guillaume , Butte Sophie , Paneitz Dane C. , Borger Michael A. , Badhwar Vinay , Sundt Thoralf M. , Langer Nathaniel B. , Melnitchouk Serguei
Published: March 2023

Background

Patients with mitral valve prolapse (MVP) requiring surgical repair (MVr) are increasingly operated using minimally invasive strategies. Skill acquisition may be facilitated by a dedicated MVr program. We present here our institutional experience in establishing minimally invasive MVr (starting in 2014), laying the foundation to introduce robotic MVr.

Methods

We reviewed all patients that had undergone MVr for MVP via sternotomy or mini-thoracotomy between January 2013 and December 2020 at our institution. In addition, all cases of robotic MVr between January 2021 and August 2022 were analyzed. Case complexity, repair techniques, and outcomes are presented for the conventional sternotomy, right mini-thoracotomy and robotic approaches. A subgroup analysis comparing only isolated MVr cases via sternotomy vs. right mini-thoracotomy was conducted using propensity score matching.

Results

Between 2013 and 2020, 799 patients were operated for native MVP at our institution, of which 761 (95.2%) received planned MVr (263 [34.6%] via mini-thoracotomy) and 38 (4.8%) received planned MV replacement. With increasing proportions of minimally invasive procedures (2014: 14.8%, 2020: 46.5%), we observed a continuous growth in overall institutional volume of MVP (n = 69 in 2013; n = 127 in 2020) and markedly improved institutional rates of successful MVr, with 95.4% in 2013 vs. 99.2% in 2020. Over this period, a higher complexity of cases were treated minimally-invasively and increased use of neochord implantation ± limited leaflet resection was observed. Patients operated minimally invasively had longer aortic cross-clamp times (94 vs. 88 min, p = 0.001) but shorter ventilation times (4.4 vs. 4.8 h, p = 0.002) and hospital stays (5 vs. 6 days, p < 0.001) than those operated via sternotomy, with no significant differences in other outcome variables. A total of 16 patients underwent robotically assisted MVr with successful repair in all cases.

Conclusion

A focused approach towards minimally invasive MVr has transformed the overall MVr strategy (incision; repair techniques) at our institution, leading to a growth in MVr volume and improved repair rates without significant complications. On this foundation, robotic MVr was first introduced at our institution in 2021 with excellent outcomes. This emphasizes the importance of building a competent team to perform these challenging operations, especially during the initial learning curve.
Forward modeling of P- and S-waves response of fractures intersected with horizontal wells in tight reservoirs
Authors: Fan Huajun , Liu Peng , Zhao Hao , Zhang Mengsheng , Liao Maojie , Zhong Guanghai , Wang Kewen , Wu Hongliang
Published: March 2023

Horizontal wells play an important role in expanding the drilling volume of reservoirs and oil production area, and are widely used in unconventional reservoirs. Fractures have a positive effect on reservoir permeability, but fractures can also cause accidents such as casing deformation and inter-well frac-hit. It is of great significance to identify and evaluate fractures intersected with horizontal wells in tight reservoirs. In this paper, a three-dimensional numerical model of horizontal wells and fractures in tight reservoirs is designed. The responses of monopole P-wave and dipole S-wave to fractures with different width, dip angle and filling medium are systematically studied, by using three-dimensional finite difference algorithm. The results show that when the fracture is filled with calcite, the amplitude attenuation of monopole P-wave and dipole S-wave has a monotonic exponential increase with the increase of fracture width and the decrease of fracture dip angle. In the real data processing, the amplitude attenuation of P- and S-waves can be used to jointly evaluate the fracture filled with calcite. When the fracture is filled with water, both P- and S-waves have prominent amplitude attenuation. P wave amplitude attenuation does not have a monotonic variation with the increase of fracture width but it has a monotonic increase with the decrease of fracture dip angle. S wave amplitude attenuation has a monotonic increase with the increase of fracture width and the decrease of fracture dip angle. The amplitude attenuation of P- and S- waves rises significantly when the fracture is filled with natural gas. This study is crucial for better understanding the response of P- and S-waves to fractures intersected with borehole in tight reservoirs, and it provides useful information for the inversion of fracture parameters by using P- and S-waves.
Study on heat exchange of groundwater under complex geological conditions in karst area of south China
Authors: Wang Kun , Wang Wanjiang , Huang Lidong
Published: March 2023

This paper takes a groundwater source heat pump in the region as the research object and based on field research, experimental tests combined with comparative analysis, the data on its operation is monitored and analyzed in terms of operation, energy saving, and environment. The results show that the cooling temperatures of the test rooms were all below 26°C, the average coefficient of performance of the units was 4.61–4.93 and the average coefficient of performance of the system was 3.08–3.27. In addition, compared to conventional water-cooled chillers, 466 tons of standard coal could be saved in one cooling season, resulting in a reduction of 1,150.8 tons of carbon dioxide emissions, 9.3 tons of sulfur dioxide emissions and 4.7 tons of dust emissions The savings in operating costs are 793,000 RMB. This shows that the groundwater source heat pump has good energy efficiency and economy. The research results obtained in this paper provide a reference for improving energy efficiency and optimizing the operation of the groundwater source heat pump system. It is of great significance to the application of groundwater source heat pump systems in areas with complex geological environments.
Case Report: Surgery and genetic analysis of a complete androgen insensitivity syndrome family with testicular malignant tumors
Authors: Jiang Lu , Jia Peng , Duan Baofeng , Zhang Yan
Published: March 2023

Introduction: Complete androgen insensitivity syndrome (CAIS) is a rare sex development disorder that results from X-linked androgen receptor gene mutations. Malignant transformation of the gonads is the most feared complication in postpubertal patients.

Methods: In the current report, primary amenorrhea, infertility, and groin mass were symptoms described by a 58-year-old woman and his younger sister. Their two aunts, who shared the same clinical traits, passed away for an unknown reason.

Results: After gonadectomy, both patients were diagnosed with seminoma and an extratesticular benign tumor, and the elder sister suffered from breast cancer about a year after the operation. The diagnosis of CAIS was verified by whole-exome sequencing (WES), in which an uncommon mutation (c.2197G>A) in the AR gene was identified.

Discussion: This is the first family report of CAIS with germ cell tumors. The identified AR gene mutation based on WES can expand the understanding of CAIS.
A novel SETD2 variant causing global development delay without overgrowth in a Chinese 3-year-old boy
Authors: Wu Yuanyuan , Liu Fang , Wan Ruihua , Jiao Baoquan
Published: March 2023

Background: Luscan-Lumish syndrome is characterized by macrocephaly, postnatal overgrowth, intellectual disability (ID), developmental delay (DD), which is caused by heterozygous SETD2 (SET domain containing 2) mutations. The incidence of Luscan-Lumish syndrome is unclear. The study was conducted to provide a novel pathogenic SETD2 variant causing atypical Luscan-Lumish syndrome and review all the published SETD2 mutations and corresponding symptoms, comprehensively understanding the phenotypes and genotypes of SETD2 mutations.

Methods: Peripheral blood samples of the proband and his parents were collected for next-generation sequencing including whole-exome sequencing (WES), copy number variation (CNV) detection and mitochondrial DNA sequencing. Identified variant was verified by Sanger sequencing. Conservative analysis and structural analysis were performed to investigate the effect of mutation. Public databases such as PubMed, Clinvar and Human Gene Mutation Database (HGMD) were used to collect all cases with SETD2 mutations.

Results: A novel pathogenic SETD2 variant (c.5835_c.5836insAGAA, p. A1946Rfs*2) was identified in a Chinese 3-year-old boy, who had speech and motor delay without overgrowth. Conservative analysis and structural analysis showed that the novel pathogenic variant would loss the conserved domains in the C-terminal region and result in loss of function of SETD2 protein. Frameshift mutations and non-sense mutations account for 68.5% of the total 51 SETD2 point mutations, suggesting that Luscan-Lumish syndrome is likely due to loss of function of SETD2. But we failed to find an association between genotype and phenotype of SETD2 mutations.

Conclusion: Our findings expand the genotype-phenotype knowledge of SETD2-associated neurological disorder and provide new evidence for further genetic counselling.
PRAME and CTCFL-reactive TCRs for the treatment of ovarian cancer
Authors: van Amerongen Rosa A. , Tuit Sander , Wouters Anne K. , van de Meent Marian , Siekman Sterre L. , Meeuwsen Miranda H. , Wachsmann Tassilo L. A. , Remst Dennis F. G. , Hagedoorn Renate S. , van der Steen Dirk M. , de Ru Arnoud H. , Verdegaal Els M. E. , van Veelen Peter A. , Falkenburg J. H. Frederik , Heemskerk Mirjam H. M.
Published: March 2023

Recurrent disease emerges in the majority of patients with ovarian cancer (OVCA). Adoptive T-cell therapies with T-cell receptors (TCRs) targeting tumor-associated antigens (TAAs) are considered promising solutions for less-immunogenic ‘cold’ ovarian tumors. In order to treat a broader patient population, more TCRs targeting peptides derived from different TAAs binding in various HLA class I molecules are essential. By performing a differential gene expression analysis using mRNA-seq datasets, PRAME, CTCFL and CLDN6 were selected as strictly tumor-specific TAAs, with high expression in ovarian cancer and at least 20-fold lower expression in all healthy tissues of risk. In primary OVCA patient samples and cell lines we confirmed expression and identified naturally expressed TAA-derived peptides in the HLA class I ligandome. Subsequently, high-avidity T-cell clones recognizing these peptides were isolated from the allo-HLA T-cell repertoire of healthy individuals. Three PRAME TCRs and one CTCFL TCR of the most promising T-cell clones were sequenced, and transferred to CD8+ T cells. The PRAME TCR-T cells demonstrated potent and specific antitumor reactivity in vitro and in vivo. The CTCFL TCR-T cells efficiently recognized primary patient-derived OVCA cells, and OVCA cell lines treated with demethylating agent 5-aza-2′-deoxycytidine (DAC). The identified PRAME and CTCFL TCRs are promising candidates for the treatment of patients with ovarian cancer, and are an essential addition to the currently used HLA-A*02:01 restricted PRAME TCRs. Our selection of differentially expressed genes, naturally expressed TAA peptides and potent TCRs can improve and broaden the use of T-cell therapies for patients with ovarian cancer or other PRAME or CTCFL expressing cancers.
Inflammation markers and the risk of hypertension in people living with HIV
Authors: Ou-Yang Hui , Fu Hai-Yan , Luo Yu , Xu Zhao-Yuan , Liu Jun , Gao Rui , Duan Jin-Yu , Mao Ya-Chao , Li Hong-Juan , Du Ying-Rong
Published: March 2023

Background

The incidence of hypertension is high in people living with HIV (PLWH). High-sensitivity C-reactive protein (hsCRP), systemic inflammation response index (SIRI), and neutrophil-to-monocyte ratio (NMR) are considered economic and convenient parameters that reflect the levels of inflammation in patients. Our aim was to explore whether indirect inflammation markers are associated with hypertension in PLWH.

Methods

This was a case-control study. The case group (hypertension) comprised PLWH with hypertension, and the control group (non-hypertension) comprised sex- and age-(± 3 years)-matched PLWH without hypertension. Demographic parameters, hsCRP, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), systemic immune- inflammation index (SII), SIRI, lymphocyte-to-monocyte ratio (LMR), platelet-to-neutrophil ratio (PNR), platelet-to-monocyte ratio (PMR), NMR, time to HIV diagnosis, antiretroviral therapy (ART) duration, recent CD4+ and CD8+ cell counts, recent CD4+/CD8+ ratio, recent HIV viral load (HIV-RNA),and recent ART regimen were obtained from the patients’ electronic medical records. A t-test or Wilcoxon rank-sum test was performed to compare differences between the two groups, and conditional logistic regression was used to analyze the risk factors of hypertension. Correlations between inflammation markers and CD4+ cell counts, CD8+ cell counts, and CD4+/CD8+ ratio were analyzed using Spearman’s correlation.

Results

In the hypertension group, body mass index (BMI), hsCRP, NLR, SII, SIRI, NMR, time to HIV diagnosis, ART duration, CD4+ and CD8+ cell counts, and CD4+/CD8+ ratio, the ratio of HIV-RNA < 100 copies/mL were all higher than those in the non-hypertension group, while the PNR was lower than that in the non-hypertension group. ART duration, CD4+ cell counts, HIV-RNA < 100 copies/mL, hsCRP, SIRI, and NMR were positively associated with hypertensive risk in PLWH. CD8+ cell counts and CD4+/CD8+ ratio was negatively associated with hypertensive risk in PLWH. SIRI was negatively correlated with CD4+ cell counts and CD8+ cell counts, but positively correlated with CD4+/CD8+ ratio.

Conclusions

We identified positive associations between inflammation markers hsCRP, SIRI, NMR and hypertensive risk in PLWH. Alleviating inflammation may help control or delay the occurrence of hypertension in PLWH.