bluuebunny/crossref_metadata_2025 huggingface dataset:
Terminal Synergetic Control for Direct Active and Reactive Powers in Asynchronous Generator-Based Dual-Rotor Wind Power Systems
Authors: Benbouhenni Habib , Bizon Nicu
Published: August 2021

A terminal synergetic control (TSC) is designed in this work for a rotor side converter (RSC) of asynchronous generator (ASG)-based dual-rotor wind power (DRWP) systems. The design is based on a novel sliding manifold and aims at improving the ASG performance while minimizing active and reactive power undulations. The method performance and its effectiveness were studied under harmonic distortion (THD) of current, parameter variations and power undulations. Simulation results, carried out using Matlab software, confirmed the system’s robustness against parameter variations and its effectiveness in power undulations. The performance of the designed technique was further compared to that of integral-proportional (PI) controllers in terms of parameter variations, power undulations and THD value of current. While both controllers were able to reduce the effects of power undulations and protect the rotor circuit against over-currents, the proposed TSC was shown to be more effective than the classical PI controller in tracking power and minimizing the undulations effect.
Investigating the Feasibility of Virtual Reality (VR) for Teaching Cardiac Morphology
Authors: Pajaziti Endrit , Schievano Silvia , Sauvage Emilie , Cook Andrew , Capelli Claudio
Published: August 2021

Congenital heart disease (CHD) is the most common defect at birth. Effective training for clinical professionals is essential in order to provide a high standard of care for patients. Visual aids for teaching complex CHD have remained mostly unchanged in recent years, with traditional methods such as diagrams and specimens still essential for delivering educational content. Diagrams and other 2D visualisations for teaching are in most cases artistic illustrations with no direct relation to true, 3D medical data. Specimens are rare, difficult for students to access and are limited to specific institutions. Digital, patient-specific models could potentially address these problems within educational programmes. Virtual Reality (VR) can facilitate the access to digital models and enhance the educational experience. In this study, we recorded and analysed the sentiment of clinical professionals towards VR when learning about CHD. A VR application (VheaRts) containing a set of patient-specific models was developed in-house. The application was incorporated into a specialised cardiac morphology course to assess the feasibility of integrating such a tool, and to measure levels of acceptance. Attendees were clinical professionals from a diverse range of specialities. VR allowed users to interact with six different patient-derived models immersed within a 3D space. Feedback was recorded for 58 participants. The general response towards the use of VR was overwhelmingly positive, with 88% of attendees rating 4 or 5 for ‘helpfulness of VR in learning CHD’ (5-points Likert scale). Additionally, 70% of participants with no prior VR experience rated 4 or 5 for ‘intuitiveness and ease of use’. Our study indicates that VR has a high level of acceptance amongst clinical trainees when used as an effective aid for learning congenital heart disease. Additionally, we noted three specific use-cases where VR offered novel teaching experiences not possible with conventional methods.
Design of a Bézier-Profile Plate for Closely Spaced Multimodal Vibration Energy Harvesting
Authors: Nguyen Hai-Dang , Wang Dung-An
Published: August 2021

A cubic Bézier-profile plate for multimodal vibration energy harvesting was developed. The design of the plate was based on an optimization procedure in which the profile of the plate was optimized via the parameters of a cubic Bézier curve to meet the requirements. The multimodal energy harvesting of the plate exploited its first bending mode and its first twisting mode. The conversion of vibration energy into electrical energy was by electromagnetic induction with a magnet attached to a corner of the plate. These two closely spaced vibration modes achieved the multi-modal energy harvesting of the device. Prototypes of the device were manufactured using a numerical-control machining process. The experimental results were in good agreement with the design specifications. With the same base lengths, height, and thickness, the maximum von Mises stress of the proposed plate was much lower due to its bell-shaped profile. The cubic Bézier curve chosen for the plate profile was effective for design of the closely-spaced multimodal vibration energy harvester. With the flexibility of its controllable parametric curve, a high design freedom of the energy harvester with specified frequency ratios could be achieved.
Real Driving Emissions—Conception of a Data-Driven Calibration Methodology for Hybrid Powertrains Combining Statistical Analysis and Virtual Calibration Platforms
Authors: Krysmon Sascha , Dorscheidt Frank , Claßen Johannes , Düzgün Marc , Pischinger Stefan
Published: August 2021

The combination of different propulsion and energy storage systems for hybrid vehicles is changing the focus in the field of powertrain calibration. Shorter time-to-market as well as stricter legal requirements regarding the validation of Real Driving Emissions (RDE) require the adaptation of current procedures and the implementation of new technologies in the powertrain development process. In order to achieve highest efficiencies and lowest pollutant emissions at the same time, the layout and calibration of the control strategies for the powertrain and the exhaust gas aftertreatment system must be precisely matched. An optimal operating strategy must take into account possible trade-offs in fuel consumption and emission levels, both under highly dynamic engine operation and under extended environmental operating conditions. To achieve this with a high degree of statistical certainty, the combination of advanced methods and the use of virtual test benches offers significant potential. An approach for such a combination is presented in this paper. Together with a Hardware-in-the-Loop (HiL) test bench, the novel methodology enables a targeted calibration process, specifically designed to address calibration challenges of hybridized powertrains. Virtual tests executed on a HiL test bench are used to efficiently generate data characterizing the behavior of the system under various conditions with a statistically based evaluation identifying white spots in measurement data, used for calibration and emission validation. In addition, critical sequences are identified in terms of emission intensity, fuel consumption or component conditions. Dedicated test scenarios are generated and applied on the HiL test bench, which take into account the state of the system and are adjusted depending on it. The example of one emission calibration use case is used to illustrate the benefits of using a HiL platform, which achieves approximately 20% reduction in calibration time by only showing differences of less than 2% for fuel consumption and emission levels compared to real vehicle tests.
Detection of Changes in Total Antioxidant Capacity, the Content of Polyphenols, Caffeine, and Heavy Metals of Teas in Relation to Their Origin and Fermentation
Authors: Bobková Alica , Demianová Alžbeta , Belej Ľubomír , Harangozo Ľuboš , Bobko Marek , Jurčaga Lukáš , Poláková Katarína , Božiková Monika , Bilčík Matúš , Árvay Július
Published: August 2021

Tea (Camellia sinensis) is widely sought for beverages worldwide. Heavy metals are often the main aims of the survey of teas, given that the use of agricultural fertilization is very frequent. Some of these may affect the content of bioactive compounds. Therefore, in this study, we analyzed fermented and non-fermented teas of a single plant origin from Japan, Nepal, Korea, and China, and described mutual correlations and changes in the total antioxidant capacity (TAC), and the content of polyphenols (TPC), caffeine, and heavy metals in tea leaves, in relation to the origin and fermentation process. Using UV-VIS spectrophotometry and HPLC-DAD, we determined variations in bioactive compounds’ content in relation to the fermentation process and origin and observed negative correlations between TAC and TPC. Heavy metal content followed this order: Mn > Fe > Cu > Zn > Ni > Cr > Pb > Co > Cd > Hg. Given the homogenous content of these elements in relation to fermentation, this paper also describes the possibility of using heavy metals as determinants of geographical origin. Linear Discriminant Analysis showed an accuracy of 75% for Ni, Co, Cd, Hg, and Pb, explaining 95.19% of the variability between geographical regions.
The Influence of Corporate Networks on the Competitive Advantage of High Technology Enterprises in China: The Mediating Effects of Dynamic Capacities and Ambidextrous Combination
Authors: Wang Huayun , Zhang Zhuoran
Published: August 2021

As an essential channel to obtain external resources and information, corporate networks have played a key role in enhancing the competitive advantage of firms, especially during the period where most of the high-technology firms in China started to directly seek means other than import technology to boost their competitive advantages. However, there was still ambiguity concerning how exactly corporate networks would affect comparative advantage. This study sought to expose the internal mechanisms among two aspects of corporate networks, namely, the network strength and the network centrality, and competitive advantage. We also examined the mediating effects of the dynamic capabilities and the ambidextrous combination. Managers of 384 high technology enterprises in China were interviewed via telephone calls during the period of January to June 2020. The data were analyzed by utilizing the structural equation method, and the results show that both dynamic capacities and the ambidextrous combination significantly mediate the relationship between corporate networks and firms’ comparative advantage, where the two mediators also had a significant relationship with each other. Moreover, the multigroup analysis also unveiled that the corporate networks had a greater impact on competitive advantage and the ambidextrous combination in the manufacturing sector, while small-sized enterprises and service enterprises would benefit more than others from the improvement in the dynamic capabilities and ambidextrous combination. Our findings fill the gap in the literature and provide useful information to firms in China on how to allocate internal and external resources to enhance their competitive advantages.
SPPD: A Novel Reassembly Method for 3D Terracotta Warrior Fragments Based on Fracture Surface Information
Authors: Yao Wenmin , Chu Tong , Tang Wenlong , Wang Jingyu , Cao Xin , Zhao Fengjun , Li Kang , Geng Guohua , Zhou Mingquan
Published: August 2021

As one of China′s most precious cultural relics, the excavation and protection of the Terracotta Warriors pose significant challenges to archaeologists. A fairly common situation in the excavation is that the Terracotta Warriors are mostly found in the form of fragments, and manual reassembly among numerous fragments is laborious and time-consuming. This work presents a fracture-surface-based reassembling method, which is composed of SiamesePointNet, principal component analysis (PCA), and deep closest point (DCP), and is named SPPD. Firstly, SiamesePointNet is proposed to determine whether a pair of point clouds of 3D Terracotta Warrior fragments can be reassembled. Then, a coarse-to-fine registration method based on PCA and DCP is proposed to register the two fragments into a reassembled one. The above two steps iterate until the termination condition is met. A series of experiments on real-world examples are conducted, and the results demonstrate that the proposed method performs better than the conventional reassembling methods. We hope this work can provide a valuable tool for the virtual restoration of three-dimension cultural heritage artifacts.
LINC01133 Inhibits Invasion and Promotes Proliferation in an Endometriosis Epithelial Cell Line
Authors: Yotova Iveta , Hudson Quanah J. , Pauler Florian M. , Proestling Katharina , Haslinger Isabella , Kuessel Lorenz , Perricos Alexandra , Husslein Heinrich , Wenzl René
Published: August 2021

Endometriosis is a common gynecological disorder characterized by ectopic growth of endometrium outside the uterus and is associated with chronic pain and infertility. We investigated the role of the long intergenic noncoding RNA 01133 (LINC01133) in endometriosis, an lncRNA that has been implicated in several types of cancer. We found that LINC01133 is upregulated in ectopic endometriotic lesions. As expression appeared higher in the epithelial endometrial layer, we performed a siRNA knockdown of LINC01133 in an endometriosis epithelial cell line. Phenotypic assays indicated that LINC01133 may promote proliferation and suppress cellular migration, and affect the cytoskeleton and morphology of the cells. Gene ontology analysis of differentially expressed genes indicated that cell proliferation and migration pathways were affected in line with the observed phenotype. We validated upregulation of p21 and downregulation of Cyclin A at the protein level, which together with the quantification of the DNA content using fluorescence-activated cell sorting (FACS) analysis indicated that the observed effects on cellular proliferation may be due to changes in cell cycle. Further, we found testis-specific protein kinase 1 (TESK1) kinase upregulation corresponding with phosphorylation and inactivation of actin severing protein Cofilin, which could explain changes in the cytoskeleton and cellular migration. These results indicate that endometriosis is associated with LINC01133 upregulation, which may affect pathogenesis via the cellular proliferation and migration pathways.
Retinal Pigment Epithelium Expressed Toll-like Receptors and Their Potential Role in Age-Related Macular Degeneration
Authors: Klettner Alexa , Roider Johann
Published: August 2021

(1) Background: Inflammation is a major pathomechanism in the development and progression of age-related macular degeneration (AMD). The retinal pigment epithelium (RPE) may contribute to retinal inflammation via activation of its Toll-like receptors (TLR). TLR are pattern recognition receptors that detect the pathogen- or danger-associated molecular pattern. The involvement of TLR activation in AMD is so far not understood. (2) Methods: We performed a systematic literature research, consulting the National Library of Medicine (PubMed). (3) Results: We identified 106 studies, of which 54 were included in this review. Based on these studies, the current status of TLR in AMD, the effects of TLR in RPE activation and of the interaction of TLR activated RPE with monocytic cells are given, and the potential of TLR activation in RPE as part of the AMD development is discussed. (4) Conclusion: The activation of TLR2, -3, and -4 induces a profound pro-inflammatory response in the RPE that may contribute to (long-term) inflammation by induction of pro-inflammatory cytokines, reducing RPE function and causing RPE cell degeneration, thereby potentially constantly providing new TLR ligands, which could perpetuate and, in the long run, exacerbate the inflammatory response, which may contribute to AMD development. Furthermore, the combined activation of RPE and microglia may exacerbate neurotoxic effects.
Gran1: A Granulysin-Derived Peptide with Potent Activity against Intracellular Mycobacterium tuberculosis
Authors: Noschka Reiner , Wondany Fanny , Kizilsavas Gönül , Weil Tanja , Weidinger Gilbert , Walther Paul , Michaelis Jens , Stenger Steffen
Published: August 2021

Granulysin is an antimicrobial peptide (AMP) expressed by human T-lymphocytes and natural killer cells. Despite a remarkably broad antimicrobial spectrum, its implementation into clinical practice has been hampered by its large size and off-target effects. To circumvent these limitations, we synthesized a 29 amino acid fragment within the putative cytolytic site of Granulysin (termed “Gran1”). We evaluated the antimicrobial activity of Gran1 against the major human pathogen Mycobacterium tuberculosis (Mtb) and a panel of clinically relevant non-tuberculous mycobacteria which are notoriously difficult to treat. Gran1 efficiently inhibited the mycobacterial proliferation in the low micro molar range. Super-resolution fluorescence microscopy and scanning electron microscopy indicated that Gran1 interacts with the surface of Mtb, causing lethal distortions of the cell wall. Importantly, Gran1 showed no off-target effects (cytokine release, chemotaxis, cell death) in primary human cells or zebrafish embryos (cytotoxicity, developmental toxicity, neurotoxicity, cardiotoxicity). Gran1 was selectively internalized by macrophages, the major host cell of Mtb, and restricted the proliferation of the pathogen. Our results demonstrate that the hypothesis-driven design of AMPs is a powerful approach for the identification of small bioactive compounds with specific antimicrobial activity. Gran1 is a promising component for the design of AMP-containing nanoparticles with selective activity and favorable pharmacokinetics to be pushed forward into experimental in vivo models of infectious diseases, most notably tuberculosis.
Mesenchymal Stem/Stromal Cells and Their Derivates in Acute Diseases: Emergency in the Post-COVID-19 Times
Authors: Vizoso Francisco J. , Fernández-Francos Silvia , Eiro Noemi
Published: August 2021

The current coronavirus disease-19 (COVID-19) pandemic has strongly revived the pressing need to incorporate new therapeutic alternatives to deal with medical situations that result in a dramatic breakdown in the body’s normal homeostasis [...]
Rootstocks Overexpressing StNPR1 and StDREB1 Improve Osmotic Stress Tolerance of Wild-Type Scion in Transgrafted Tobacco Plants
Authors: Hezema Yasmine S. , Shukla Mukund R. , Goel Alok , Ayyanath Murali M. , Sherif Sherif M. , Saxena Praveen K.
Published: August 2021

In grafted plants, the movement of long-distance signals from rootstocks can modulate the development and function of the scion. To understand the mechanisms by which tolerant rootstocks improve scion responses to osmotic stress (OS) conditions, mRNA transport of osmotic responsive genes (ORGs) was evaluated in a tomato/potato heterograft system. In this system, Solanum tuberosum was used as a rootstock and Solanum lycopersicum as a scion. We detected changes in the gene expression levels of 13 out of the 21 ORGs tested in the osmotically stressed plants; of these, only NPR1 transcripts were transported across the graft union under both normal and OS conditions. Importantly, OS increased the abundance of StNPR1 transcripts in the tomato scion. To examine mRNA mobility in transgrafted plants, StNPR1 and StDREB1 genes representing the mobile and non-mobile transcripts, respectively, were overexpressed in tobacco (Nicotiana tabacum). The evaluation of transgenic tobacco plants indicated that overexpression of these genes enhanced the growth and improved the physiological status of transgenic plants growing under OS conditions induced by NaCl, mannitol and polyethylene glycol (PEG). We also found that transgenic tobacco rootstocks increased the OS tolerance of the WT-scion. Indeed, WT scions on transgenic rootstocks had higher ORGs transcript levels than their counterparts on non-transgenic rootstocks. However, neither StNPR1 nor StDREB1 transcripts were transported from the transgenic rootstock to the wild-type (WT) tobacco scion, suggesting that other long-distance signals downstream these transgenes could have moved across the graft union leading to OS tolerance. Overall, our results signify the importance of StNPR1 and StDREB1 as two anticipated candidates for the development of stress-resilient crops through transgrafting technology.
Biochemical Characterization of a Novel Exo-Type PL7 Alginate Lyase VsAly7D from Marine Vibrio sp. QY108
Authors: Zhang Fengchao , Fu Zheng , Tang Luyao , Zhang Zhelun , Han Feng , Yu Wengong
Published: August 2021

Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L-erythro-5-hexoseulose uronate (DEH) by a non-enzyme reaction, which is an important raw material for the preparation of bioethanol. In our research, a novel exo-type alginate lyase, VsAly7D, belonging to the PL7 family was isolated from marine bacterium Vibrio sp. QY108 and recombinantly expressed in Escherichia coli. The purified VsAly7D demonstrated the highest activity at 35 °C, whereas it still maintained 46.5% and 83.1% of its initial activity at 20 °C and 30 °C, respectively. In addition, VsAly7D exhibited the maximum activity under alkaline conditions (pH 8.0), with the simultaneously remaining stability between pH 8.0 and 10.0. Compared with other reported exo-type enzymes, VsAly7D could efficiently degrade alginate, poly-β-D-mannuronate (polyM) and poly-α-L-guluronate (polyG) with highest specific activities (663.0 U/mg, 913.6 U/mg and 894.4 U/mg, respectively). These results showed that recombinant VsAly7D is a suitable tool enzyme for unsaturated alginate monosaccharide preparation and holds great promise for producing bioethanol from brown algae.
Human CD4+ T-Cell Clone Expansion Leads to the Expression of the Cysteine Peptidase Inhibitor Cystatin F
Authors: Perišić Nanut Milica , Pawelec Graham , Kos Janko
Published: August 2021

The existence of CD4+ cytotoxic T cells (CTLs) at relatively high levels under different pathological conditions in vivo suggests their role in protective and/or pathogenic immune functions. CD4+ CTLs utilize the fundamental cytotoxic effector mechanisms also utilized by CD8+ CTLs and natural killer cells. During long-term cultivation, CD4+ T cells were also shown to acquire cytotoxic functions. In this study, CD4+ human T-cell clones derived from activated peripheral blood lymphocytes of healthy young adults were examined for the expression of cytotoxic machinery components. Cystatin F is a protein inhibitor of cysteine cathepsins, synthesized by CD8+ CTLs and natural killer cells. Cystatin F affects the cytotoxic efficacy of these cells by inhibiting the major progranzyme convertases cathepsins C and H as well as cathepsin L, which is involved in perforin activation. Here, we show that human CD4+ T-cell clones express the cysteine cathepsins that are involved in the activation of granzymes and perforin. CD4+ T-cell clones contained both the inactive, dimeric form as well as the active, monomeric form of cystatin F. As in CD8+ CTLs, cysteine cathepsins C and H were the major targets of cystatin F in CD4+ T-cell clones. Furthermore, CD4+ T-cell clones expressed the active forms of perforin and granzymes A and B. The levels of the cystatin F decreased with time in culture concomitantly with an increase in the activities of granzymes A and B. Therefore, our results suggest that cystatin F plays a role in regulating CD4+ T cell cytotoxicity. Since cystatin F can be secreted and taken up by bystander cells, our results suggest that CD4+ CTLs may also be involved in regulating immune responses through cystatin F secretion.
Adaptor Molecules Epitranscriptome Reprograms Bacterial Pathogenicity
Authors: Kouvela Adamantia , Zaravinos Apostolos , Stamatopoulou Vassiliki
Published: August 2021

The strong decoration of tRNAs with post-transcriptional modifications provides an unprecedented adaptability of this class of non-coding RNAs leading to the regulation of bacterial growth and pathogenicity. Accumulating data indicate that tRNA post-transcriptional modifications possess a central role in both the formation of bacterial cell wall and the modulation of transcription and translation fidelity, but also in the expression of virulence factors. Evolutionary conserved modifications in tRNA nucleosides ensure the proper folding and stability redounding to a totally functional molecule. However, environmental factors including stress conditions can cause various alterations in tRNA modifications, disturbing the pathogen homeostasis. Post-transcriptional modifications adjacent to the anticodon stem-loop, for instance, have been tightly linked to bacterial infectivity. Currently, advances in high throughput methodologies have facilitated the identification and functional investigation of such tRNA modifications offering a broader pool of putative alternative molecular targets and therapeutic avenues against bacterial infections. Herein, we focus on tRNA epitranscriptome shaping regarding modifications with a key role in bacterial infectivity including opportunistic pathogens of the human microbiome.
Anticancer Activity and Mechanisms of Action of New Chimeric EGFR/HDAC-Inhibitors
Authors: Goehringer Nils , Biersack Bernhard , Peng Yayi , Schobert Rainer , Herling Marco , Ma Andi , Nitzsche Bianca , Höpfner Michael
Published: August 2021

New chimeric inhibitors targeting the epidermal growth factor (EGFR) and histone deacetylases (HDACs) were synthesized and tested for antineoplastic efficiency in solid cancer (prostate and hepatocellular carcinoma) and leukemia/lymphoma cell models. The most promising compounds, 3BrQuin-SAHA and 3ClQuin-SAHA, showed strong inhibition of tumor cell growth at one-digit micromolar concentrations with IC50 values similar to or lower than those of clinically established reference compounds SAHA and gefitinib. Target-specific EGFR and HDAC inhibition was demonstrated in cell-free kinase assays and Western blot analyses, while unspecific cytotoxic effects could not be observed in LDH release measurements. Proapoptotic formation of reactive oxygen species and caspase-3 activity induction in PCa and HCC cell lines DU145 and Hep-G2 seem to be further aspects of the modes of action. Antiangiogenic potency was recognized after applying the chimeric inhibitors on strongly vascularized chorioallantoic membranes of fertilized chicken eggs (CAM assay). The novel combination of two drug pharmacophores against the EGFR and HDACs in one single molecule was shown to have pronounced antineoplastic effects on tumor growth in both solid and leukemia/lymphoma cell models. The promising results merit further investigations to further decipher the underlying modes of action of the novel chimeric inhibitors and their suitability for new clinical approaches in tumor treatment.
Do All Integrase Strand Transfer Inhibitors Have the Same Lipid Profile? Review of Randomised Controlled Trials in Naïve and Switch Scenarios in HIV-Infected Patients
Authors: Saumoy Maria , Sanchez-Quesada Jose Luís , Ordoñez-Llanos Jordi , Podzamczer Daniel
Published: August 2021

In this study, we aim to explore the effects on lipids of integrase strand transfer inhibitors (INSTIs) in naïve and switch randomised controlled trials, and compare them with protease inhibitors (PIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs). We reviewed phase 3/4 randomised clinical trials in the Cochrane and PubMed databases that compare an INSTI with a boosted PI, an NNRTI, or another INSTI plus one or two nucleoside/nucleotide reverse transcriptase inhibitors (NtRTIs) in naïve patients and switching strategies in HIV-infected patients. We reported the baseline plasma concentration of total cholesterol (TC), low and high-density lipoprotein cholesterol (LDL-c, HDL-c), triglycerides (TG), and the TC/HDL-c ratio, as well as the change at weeks 48 and 96, when available. In naïve HIV-infected patients, raltegravir (RAL) and dolutegravir (DTG) have a more favourable lipid profile compared with NNRTI and boosted PI. Elvitegravir (EVG/c) has a superior lipid profile compared with efavirenz and is similar to that observed with ritonavir-boosted atazanavir except in TG, which increases less with EVG/c. In naïve patients, RAL, DTG, and bictegravir (BIC) produce a similar, slight increase in lipids. In switching trials, the regimen change based on a boosted PI or efavirenz to RAL, DTG, or BIC is associated with clinically significant decreases in lipids that are minor when the change is executed on EVG/c. No changes were observed in lipids by switching trials between INSTIs. In summary, RAL, DTG, and BIC have superior lipid profiles compared with boosted-PI, efavirenz, and EVG/c, in studies conducted in naïve participants, and they are associated with a clinically significant decrease in lipoproteins by switching studies.
Preliminary Results of Robotic Lobectomy in Stage IIIA-N2 NSCLC after Induction Treatment: A Case Control Study
Authors: Casiraghi Monica , Petrella Francesco , Sedda Giulia , Mazzella Antonio , Guarize Juliana , Maisonneuve Patrick , De Marinis Filippo , Spaggiari Lorenzo
Published: August 2021

Despite there already being many studies on robotic surgery as a minimally invasive approach for non-small-cell lung cancer (NSCLC) patients, the use of this technique for stage III disease is still poorly described. These are the preliminary results of our prospective study on the safety and effectiveness of robotic approaches in patients with locally advanced NSCLC in terms of postoperative complications and oncological outcomes. Since 2016, we prospectively investigated 19 consecutive patients with NSCLC stage IIIA-pN2 (diagnosed by EBUS-TBNA) who underwent lobectomy and radical lymph node dissection with robotic approaches after induction treatment. Furthermore, we matched a case-control study with 46 patients treated with open surgery during the same period of time, with similar age, comorbidities, clinical stage and tumor size. The individual matched population was composed of 16 robot-assisted thoracic surgeries and 16 patients who underwent open surgery. The median time range of resection was inferior in the open group compared to robotic lobectomy (243 vs. 161 min; p < 0.001). Lymph node resection and positivity were not significantly different (p = 0.96 and p = 0.57, respectively). Moreover, no difference was observed for PFS (p = 0.16) or OS (p = 0.41). In conclusion, we demonstrated that the early outcomes and oncological results of N2-patients after robotic lobectomy were similar to those who had open surgery. Considering the advantages of minimally invasive surgery, robot-assisted lobectomy appears to be a safe approach to patients with locally advanced diseases.
A Detection Method of Operated Fake-Images Using Robust Hashing
Authors: Tanaka Miki , Shiota Sayaka , Kiya Hitoshi
Published: August 2021

SNS providers are known to carry out the recompression and resizing of uploaded images, but most conventional methods for detecting fake images/tampered images are not robust enough against such operations. In this paper, we propose a novel method for detecting fake images, including distortion caused by image operations such as image compression and resizing. We select a robust hashing method, which retrieves images similar to a query image, for fake-image/tampered-image detection, and hash values extracted from both reference and query images are used to robustly detect fake-images for the first time. If there is an original hash code from a reference image for comparison, the proposed method can more robustly detect fake images than conventional methods. One of the practical applications of this method is to monitor images, including synthetic ones sold by a company. In experiments, the proposed fake-image detection is demonstrated to outperform state-of-the-art methods under the use of various datasets including fake images generated with GANs.
An Approach for the Transfer of Real Surfaces in Finite Element Simulations
Authors: Joerger Arn , Reichert Stefan , Wittig Christoph , Sistanizadeh Aghdam Navid , Albers Albert
Published: August 2021

Virtual simulations are a relevant element in product engineering processes and facilitate engineers to test different concepts during early phases of the development. However, in tribological product engineering, simulations are hardly used because input data such as material behavior are often missing. Besides the material behavior, the surface roughness of the contacting elements is relevant for tribological systems. To expand the capabilities of the virtual engineering of tribological components such as bearings or brakes, the hereby presented approach allows for the depiction of real rough surfaces in finite element simulations. Rough surfaces are scanned by a white-light interferometer (WLI) and further processed by removing the outliers and replacing non-measured samples. Next, a spline generation creates a solid body, which is imported to CAD software and afterwards meshed with triangle and quadrilateral elements in different sizes. The results comprise the evaluation of six differently manufactured (turned, coated, and pressed) real surfaces. The surfaces are compared by the deviations of the roughness values after measuring with the WLI and after meshing them. Furthermore, the elements’ aspect ratios and skewness describe the mesh quality. The results show that the transfer is dependent upon deep cliffs and large Sz values in comparison to the lateral expansion.
Unusual Structures and Cytotoxicities of Chitonoidosides A, A1, B, C, D, and E, Six Triterpene Glycosides from the Far Eastern Sea Cucumber Psolus chitonoides
Authors: Silchenko Alexandra S. , Kalinovsky Anatoly I. , Avilov Sergey A. , Andrijaschenko Pelageya V. , Popov Roman S. , Dmitrenok Pavel S. , Chingizova Ekaterina A. , Kalinin Vladimir I.
Published: August 2021

Six new triterpene tetra-, penta- and hexaosides, chitonoidosides A (1), A1 (2), B (3), C (4), D (5), and E (6), containing one or two sulfate groups, have been isolated from the Far-Eastern sea cucumber Psolus chitonoides, collected near Bering Island (Commander Islands) from the depth of 100–150 m. Three of the isolated compounds (1, 3 and 6) are characterized by the unusual aglycone of new type having 18(20)-ether bond and lacking a lactone in contrast with wide spread holostane derivatives. Another unexpected finding is 3-O-methylxylose residue as a terminal unit in the carbohydrate chains of chitonoidosides B (3), C (4), and E (6), which has never been found before in the glycosides from holothurians belonging to the Psolidae family. Moreover, this monosaccharide is sulfated in the compound 4 into unprecedented 3-O-methylxylose 4-O-sulfate residue. Chitonoidoside C (4) is characterized by tetrasaccharide moiety lacking a part of the bottom semi-chain, but having disaccharide fragment attached to C-4 of Xyl1. Such architecture is not common in sea cucumber glycosides. Cytotoxic activities of the compounds 1–5 against mouse and human erythrocytes and human cancer cell lines: adenocarcinoma HeLa, colorectal adenocarcinoma DLD-1, and leukemia promyeloblast HL-60 cells were studied. The cytotoxic effect of chitonoidoside d (5) was the most significant in this series due to the presence of pentasaccharide disulfated sugar chain in combination with holostane aglycone. Surprisingly, the glycosides 1 and 3, comprising the new aglycone without γ-lactone, demonstrated similar activity to the known compounds with holostane aglycones. Chitonoidoside C (4) was less cytotoxic due to the different architecture of the carbohydrate chain compared to the other glycosides and probably due to the presence of a sulfate group at C-4 in 3-O-MeXyl4.
Regulation of p53 Activity by (+)-Epiloliolide Isolated from Ulva lactuca
Authors: Chung Yuheon , Jeong Seula , Lee In-Kyoung , Yun Bong-Sik , Lee Jung Sup , Ro Seungil , Park Jong Kun
Published: August 2021

Ulva lactuca (U. lactuca) is a green alga distributed worldwide and used as a food and cosmetic material. In our previous study, we determined the effects of U. lactuca methanol extracts on the UVB-induced DNA repair. In the present study, we fractionated U. lactuca methanol extracts to identify the effective compound for the DNA repair. MTT assay demonstrated that (+)-epiloliolide showed no cytotoxicity up to 100 μM in BJ-5ta human dermal fibroblast. Upon no treatment, exposure to UVB 400 J/m2 decreased cell viability by 45%, whereas (+)-epiloliolide treatment for 24 h after UVB exposure significantly increased the cell viability. In GO and GESA analysis, a number of differentially expressed genes were uniquely expressed in (+)-epiloliolide treated cells, which were enriched in the p53 signaling pathway and excision repair. Immunofluorescence demonstrated that (+)-epiloliolide increased the nuclear localization of p53. Comet assay demonstrated that (+)-epiloliolide decreased tail moment increased by UVB. Western blot analysis demonstrated that (+)-epiloliolide decreased the levels of p-p53, p21, Bax, and Bim, but increased that of Bcl-2. Reverse transcription PCR (RT-PCR) demonstrated that (+)-epiloliolide decreased the levels of MMP 1, 9, and 13, but increased that of COL1A1. These results suggest that (+)-epiloliolide regulates p53 activity and has protective effects against UVB.
Hot Deformation Behavior and Dynamic Recrystallization of Ultra High Strength Steel
Authors: Zhong Liping , Wang Bo , Hu Chundong , Zhang Jieyu , Yao Yu
Published: August 2021

In this paper, in order to improve the microstructure uniformity of an ultra-high strength martensitic steel with a strength greater than 2500 Mpa developed by multi-directional forging in the laboratory, a single-pass hot compression experiment with the strain rate of 0.01 to 1 s−1 and a temperature of 800 to 1150 °C was conducted. Based on the experimental data, the material parameters were determined, the constitutive model considering the influence of work hardening, the recrystallization softening on the dislocation density, and the recrystallized grain size model were established. After introducing the model into the finite element software DEFORM-3D, the thermal compression experiment was simulated, and the results were consistent with the experimental results. The rule for obtaining forging stock with a uniform and refinement microstructure was acquired by comparing the simulation and the experimental results, which are helpful to formulate an appropriate forging process.
Wire Arc Additive Manufacturing with Novel Al-Mg-Si Filler Wire—Assessment of Weld Quality and Mechanical Properties
Authors: Winterkorn René , Pittner Andreas , Rethmeier Michael
Published: August 2021

Wire arc additive manufacturing enables the production of near-net shape large-volume metallic components leveraging an established industrial base of welding and cladding technology and adapting it for layer-wise material deposition. However, the complex relationship between the process parameters and resulting mechanical properties of the components still remains challenging. In case of high-strength Al-Mg-Si aluminum alloys, no commercial filler wires are yet available due the high susceptibility of solidification cracking as well as the necessary efforts to obtain acceptable mechanical properties. To address this need, we evaluated a novel filler wire based on AlMg0.7Si doped with a Ti5B1 master alloy to foster fine equiaxed grains within the deposited metal. The correlation between the process parameters and component quality was examined by analyzing the size and distribution of pores as well as the grain morphology. Furthermore, we evaluated the influence of different post-weld heat treatment strategies to achieve mechanical properties corresponding to the reference wrought material. We demonstrated that fine equiaxed grains in the weld metal reduced the susceptibility of solidification cracking significantly. The novel AlMg0.7Si-TiB (S Al 6063-TiB) filler wire facilitated wire arc additive manufacturing of high-strength aluminum components with mechanical properties that were almost as superior as the corresponding wrought base material.
An Investigation of the Miscibility Gap Controlling Phase Formation in Refractory Metal High Entropy Superalloys via the Ti-Nb-Zr Constituent System
Authors: Whitfield Tamsin E. , Wise George J. , Pickering Ed J. , Stone Howard J. , Jones Nicholas G.
Published: August 2021

Refractory metal high entropy superalloys (RSAs) have been heralded as potential new high temperature structural materials. They have nanoscale cuboidal bcc+B2 microstructures that are thought to form on quenching through a spinodal decomposition process driven by the Ta-Zr or Nb-Zr miscibility gaps, followed by ordering of one of the bcc phases. However, it is difficult to isolate the role of different elemental interactions within compositionally complex RSAs. Therefore, in this work the microstructures produced by the Nb-Zr miscibility gap within the compositionally simpler Ti-Nb-Zr constituent system were investigated. A systematic series of alloys with compositions of Ti5NbxZr95−x (x = 25–85 at.%) was studied following quenching from solution heat treatment and long duration thermal exposures at 1000, 900 and 700 °C for 1000 h. During exposures at 900 °C and above the alloys resided in a single bcc phase field. At 700 °C, alloys with 40–75 at.% Nb resided within a three phase bcc + bcc + hcp phase field and a large misfit, 4.7–5%, was present between the two bcc phases. Evidence of nanoscale cuboidal microstructures was not observed, even in slow cooled samples. Whilst it was not possible to conclusively determine whether a spinodal decomposition occurs within this ternary system, these insights suggest that Nb-Zr interactions may not play a significant role in the formation of the nanoscale cuboidal RSA microstructures during cooling.
Valorization of Byproducts of Hemp Multipurpose Crop: Short Non-Aligned Bast Fibers as a Source of Nanocellulose
Authors: Dalle Vacche Sara , Karunakaran Vijayaletchumy , Patrucco Alessia , Zoccola Marina , Douard Loreleï , Ronchetti Silvia , Gallo Marta , Schreier Aigoul , Leterrier Yves , Bras Julien , Beneventi Davide , Bongiovanni Roberta
Published: August 2021

Nanocellulose was extracted from short bast fibers, from hemp (Cannabis sativa L.) plants harvested at seed maturity, non-retted, and mechanically decorticated in a defibering apparatus, giving non-aligned fibers. A chemical pretreatment with NaOH and HCl allowed the removal of most of the non-cellulosic components of the fibers. No bleaching was performed. The chemically pretreated fibers were then refined in a beater and treated with a cellulase enzyme, followed by mechanical defibrillation in an ultrafine friction grinder. The fibers were characterized by microscopy, infrared spectroscopy, thermogravimetric analysis and X-ray diffraction after each step of the process to understand the evolution of their morphology and composition. The obtained nanocellulose suspension was composed of short nanofibrils with widths of 5–12 nm, stacks of nanofibrils with widths of 20–200 nm, and some larger fibers. The crystallinity index was found to increase from 74% for the raw fibers to 80% for the nanocellulose. The nanocellulose retained a yellowish color, indicating the presence of some residual lignin. The properties of the nanopaper prepared with the hemp nanocellulose were similar to those of nanopapers prepared with wood pulp-derived rod-like nanofibrils.
Templated Synthesis and Assembly of Two-, Three- and Six-Patch Silica Nanoparticles with a Controlled Patch-to-Particle Size Ratio
Authors: Liu Bin , Exiga Stéphanie , Duguet Etienne , Ravaine Serge
Published: August 2021

We report a fabrication route of silica nanoparticles with two, three or six patches with an easily tunable patch-to-particle size ratio. The synthetic pathway includes two main stages: the synthesis of silica/polystyrene multipod-like templates and the selective growth of their silica core through an iterative approach. Electron microscopy of the dimpled nanoparticles obtained after dissolution of the polystyrene nodules of the multipod-like nanoparticles provides evidence of the conformational growth of the silica core. Thanks to the presence of some polymer chains, which remained grafted at the bottom of the dimples after the dissolution of the PS nodules, the solvent-induced assembly of the patchy nanoparticles is performed. Chains, hexagonal suprastructures and cubic lattices are obtained from the assembly of two-, three- and six-patch silica nanoparticles, respectively. Our study can guide future work in both patchy nanoparticle synthesis and self-assembly. It also opens new routes towards the fabrication of specific classes of one-, two- and three-dimensional colloidal lattices, including complex tilings.
Phenolics-Rich Extracts of Dietary Plants as Regulators of Fructose Uptake in Caco-2 Cells via GLUT5 Involvement
Authors: Zakłos-Szyda Małgorzata , Pietrzyk Nina , Kowalska-Baron Agnieszka , Nowak Adriana , Chałaśkiewicz Katarzyna , Ratajewski Marcin , Budryn Grażyna , Koziołkiewicz Maria
Published: August 2021

The latest data link the chronic consumption of large amounts of fructose present in food with the generation of hypertension and disturbances in carbohydrate and lipid metabolism, which promote the development of obesity, non-alcoholic fatty liver disease, insulin resistance, and type 2 diabetes. This effect is possible after fructose is absorbed by the small intestine cells and, to a lesser extent, by hepatocytes. Fructose transport is dependent on proteins from the family of glucose transporters (GLUTs), among which GLUT5 selectively absorbs fructose from the intestine. In this study, we examined the effect of four phenolic-rich extracts obtained from A. graveolens, B. juncea, and M. chamomilla on fructose uptake by Caco-2 cells. Extracts from B. juncea and M. chamomilla most effectively reduced fluorescent fructose analogue (NBDF) accumulation in Caco-2, as well as downregulated GLUT5 protein levels. These preparations were able to decrease the mRNA level of genes encoding transcription factors regulating GLUT5 expression-thioredoxin-interacting protein (TXNIP) and carbohydrate-responsive element-binding protein (ChREBP). Active extracts contained large amounts of apigenin and flavonols. The molecular docking simulation suggested that some of identified phenolic constituents can play an important role in the inhibition of GLUT5-mediated fructose transport.
A Review of Self-Seeded Germanium Nanowires: Synthesis, Growth Mechanisms and Potential Applications
Authors: Garcia-Gil Adrià , Biswas Subhajit , Holmes Justin D.
Published: August 2021

Ge nanowires are playing a big role in the development of new functional microelectronic modules, such as gate-all-around field-effect transistor devices, on-chip lasers and photodetectors. The widely used three-phase bottom-up growth method utilising a foreign catalyst metal or metalloid is by far the most popular for Ge nanowire growth. However, to fully utilise the potential of Ge nanowires, it is important to explore and understand alternative and functional growth paradigms such as self-seeded nanowire growth, where nanowire growth is usually directed by the in situ-formed catalysts of the growth material, i.e., Ge in this case. Additionally, it is important to understand how the self-seeded nanowires can benefit the device application of nanomaterials as the additional metal seeding can influence electron and phonon transport, and the electronic band structure in the nanomaterials. Here, we review recent advances in the growth and application of self-seeded Ge and Ge-based binary alloy (GeSn) nanowires. Different fabrication methods for growing self-seeded Ge nanowires are delineated and correlated with metal seeded growth. This review also highlights the requirement and advantage of self-seeded growth approach for Ge nanomaterials in the potential applications in energy storage and nanoelectronic devices.