bluuebunny/crossref_metadata_2025 huggingface dataset:
Immune-Checkpoint Inhibitors in Platinum-Resistant Ovarian Cancer
Authors: Indini Alice , Nigro Olga , Lengyel Csongor György , Ghidini Michele , Petrillo Angelica , Lopez Salvatore , Raspagliesi Francesco , Trapani Dario , Khakoo Shelize , Bogani Giorgio
Published: April 2021

Platinum-resistant ovarian cancer (OC) has limited treatment options and is associated with a poor prognosis. There appears to be an overlap between molecular mechanisms responsible for platinum resistance and immunogenicity in OC. Immunotherapy with single agent checkpoint inhibitors has been evaluated in a few clinical trials with disappointing results. This has prompted exploration of immunotherapy combination strategies with chemotherapy, anti-angiogenics, poly (ADP-ribose) polymerase (PARP) inhibitors and other targeted agents. The role of immunotherapy in the treatment of platinum-resistant OC remains undefined. The aim of this review is to describe the immunobiology of OC and likely benefit from immunotherapy, discuss clinical trial data and biomarkers that warrant further exploration, as well as provide an overview of future drug development strategies.
Pregnancy and Cancer: Cellular Biology and Mechanisms Affecting the Placenta
Authors: Oliveira Melina de Moraes Santos , Salgado Carla de Moraes , Viana Lais Rosa , Gomes-Marcondes Maria Cristina Cintra
Published: April 2021

Cancer during pregnancy is rarely studied due to its low incidence (1:1000). However, as a result of different sociocultural and economic changes, women are postponing pregnancy, so the number of pregnant women with cancer has been increasing in recent years. The importance of studying cancer during pregnancy is not only based on maternal and foetal prognosis, but also on the evolutionary mechanisms of the cell biology of trophoblasts and neoplastic cells, which point out similarities between and suggest new fields for the study of cancer. Moreover, the magnitude of how cancer factors can affect trophoblastic cells, and vice versa, in altering the foetus’s nutrition and health is still a subject to be understood. In this context, the objective of this narrative review was to show that some researchers point out the importance of supplementing branched-chain amino acids, especially leucine, in experimental models of pregnancy associated with women with cancer. A leucine-rich diet may be an interesting strategy to preserve physiological placenta metabolism for protecting the mother and foetus from the harmful effects of cancer during pregnancy.
Pathway-Directed Therapy in Multiple Myeloma
Authors: John Lukas , Krauth Maria Theresa , Podar Klaus , Raab Marc-Steffen
Published: April 2021

Multiple Myeloma (MM) is a malignant plasma cell disorder with an unmet medical need, in particular for relapsed and refractory patients. Molecules within deregulated signaling pathways, including the RAS/RAF/MEK/ERK, but also the PI3K/AKT-pathway belong to the most promising evolving therapeutic targets. Rationally derived compounds hold great therapeutic promise to target tumor-specific abnormalities rather than general MM-associated vulnerabilities. This paradigm is probably best depicted by targeting mutated BRAF: while well-tolerated, remarkable responses have been achieved in selected patients by inhibition of BRAFV600E alone or in combination with MEK. Targeting of AKT has also shown promising results in a subset of patients as monotherapy or to resensitize MM-cells to conventional treatment. Approaches to target transcription factors, convergence points of signaling cascades such as p53 or c-MYC, are emerging as yet another exciting strategy for pathway-directed therapy. Informed by our increasing knowledge on the impact of signaling pathways in MM pathophysiology, rationally derived Precision-Medicine trials are ongoing. Their results are likely to once more fundamentally change treatment strategies in MM.
Achieving ‘Marginal Gains’ to Optimise Outcomes in Resectable Pancreatic Cancer
Authors: Powell-Brett Sarah , Pande Rupaly , Roberts Keith J.
Published: April 2021

Improving outcomes among patients with resectable pancreatic cancer is one of the greatest challenges of modern medicine. Major improvements in survival will result from the development of novel therapies. However, optimising existing pathways, so that patients realise benefits of already proven treatments, presents a clear opportunity to improve outcomes in the short term. This narrative review will focus on treatments and interventions where there is a clear evidence base to improve outcomes in pancreatic cancer, and where there is also evidence of variation and under-treatment. Avoidance of preoperative biliary drainage, treatment of pancreatic exocrine insufficiency, prehabiliation and enhanced recovery after surgery, reducing perioperative complications, optimising opportunities for elderly patients to receive therapy, optimising adjuvant chemotherapy and regular surveillance after surgery are some of the strategies discussed. Each treatment or pathway change represents an opportunity for marginal gain. Accumulation of marginal gains can result in considerable benefit to patients. Given that these interventions already have evidence base, they can be realised quickly and economically.
TiO2 and TiO2-Carbon Hybrid Photocatalysts for Diuron Removal from Water
Authors: Amorós-Pérez Ana , Lillo-Ródenas María Ángeles , Román-Martínez María del Carmen , García-Muñoz Patricia , Keller Nicolas
Published: April 2021

TiO2 and TiO2-activated carbon (AC) photocatalysts have been prepared (by sol-gel synthesis), characterized, and tested in the removal of diuron from water under simulated solar light. The preparation variables of the two series of catalysts are: (i) heat-treatment temperature of bare TiO2 (350, 400, 450 and 500 °C) and (ii) activated carbon content (0.5, 1, 5, and 10 wt.%) in TiO2-AC samples heat-treated at 350 °C. The activated carbon was previously prepared by hydrothermal carbonization of saccharose and has spherical shape. The heat-treatment temperature does not determine the efficiency of TiO2 for diuron photocatalytic degradation, but clearly influences the diuron adsorption capacity. The capacity of TiO2-AC samples for diuron removal increases with the carbon content and it is the result of combined diuron adsorption and photodegradation. Thus, the sample with highest carbon content (10 wt.% nominal) leads to the highest diuron removal. The TiO2-AC photocatalysts have proved to be capable of degrading diuron previously adsorbed in dark conditions, which allows their regeneration.
Alterations in the Gut-Microbial-Inflammasome-Brain Axis in a Mouse Model of Alzheimer’s Disease
Authors: Shukla Pradeep K. , Delotterie David F. , Xiao Jianfeng , Pierre Joseph F. , Rao RadhaKrishna , McDonald Michael P. , Khan Mohammad Moshahid
Published: April 2021

Alzheimer’s disease (AD), a progressive neurodegenerative disorder characterized by memory loss and cognitive decline, is a major cause of death and disability among the older population. Despite decades of scientific research, the underlying etiological triggers are unknown. Recent studies suggested that gut microbiota can influence AD progression; however, potential mechanisms linking the gut microbiota with AD pathogenesis remain obscure. In the present study, we provided a potential mechanistic link between dysbiotic gut microbiota and neuroinflammation associated with AD progression. Using a mouse model of AD, we discovered that unfavorable gut microbiota are correlated with abnormally elevated expression of gut NLRP3 and lead to peripheral inflammasome activation, which in turn exacerbates AD-associated neuroinflammation. To this end, we observe significantly altered gut microbiota compositions in young and old 5xFAD mice compared to age-matched non-transgenic mice. Moreover, 5xFAD mice demonstrated compromised gut barrier function as evident from the loss of tight junction and adherens junction proteins compared to non-transgenic mice. Concurrently, we observed increased expression of NLRP3 inflammasome and IL-1β production in the 5xFAD gut. Consistent with our hypothesis, increased gut–microbial–inflammasome activation is positively correlated with enhanced astrogliosis and microglial activation, along with higher expression of NLRP3 inflammasome and IL-1β production in the brains of 5xFAD mice. These data indicate that the elevated expression of gut–microbial–inflammasome components may be an important trigger for subsequent downstream activation of inflammatory and potentially cytotoxic mediators, and gastrointestinal NLRP3 may promote NLRP3 inflammasome-mediated neuroinflammation. Thus, modulation of the gut microbiota may be a potential strategy for the treatment of AD-related neurological disorders in genetically susceptible hosts.
Extracellular Vesicles from Infected Cells Are Released Prior to Virion Release
Authors: Kim Yuriy , Mensah Gifty A. , Al Sharif Sarah , Pinto Daniel O. , Branscome Heather , Yelamanchili Sowmya V. , Cowen Maria , Erickson James , Khatkar Pooja , Mahieux Renaud , Kashanchi Fatah
Published: April 2021

Here, we have attempted to address the timing of EV and virion release from virally infected cells. Uninfected (CEM), HIV-1-infected (J1.1), and human T cell leukemia virus-1 (HTLV-1)-infected (HUT102) cells were synchronized in G0. Viral latency was reversed by increasing gene expression with the addition of serum-rich media and inducers. Supernatants and cell pellets were collected post-induction at different timepoints and assayed for extracellular vesicle (EV) and autophagy markers; and for viral proteins and RNAs. Tetraspanins and autophagy-related proteins were found to be differentially secreted in HIV-1- and HTLV-1-infected cells when compared with uninfected controls. HIV-1 proteins were present at 6 h and their production increased up to 24 h. HTLV-1 proteins peaked at 6 h and plateaued. HIV-1 and HTLV-1 RNA production correlated with viral protein expression. Nanoparticle tracking analysis (NTA) showed increase of EV concentration over time in both uninfected and infected samples. Finally, the HIV-1 supernatant from the 6-h samples was found not to be infectious; however, the virus from the 24-h samples was successfully rescued and infectious. Overall, our data indicate that EV release may occur prior to viral release from infected cells, thereby implicating a potentially significant effect of EVs on uninfected recipient cells prior to subsequent viral infection and spread.
Effect of Agroecological Conditions on Biologically Active Compounds and Metabolome in Carrot
Authors: Koudela Martin , Schulzova Vera , Krmela Ales , Chmelarova Hana , Hajslova Jana , Novotny Cenek
Published: April 2021

Carrot serves as a source of health-beneficial phytochemicals for human diet whose content is affected by agroecological conditions. The effect of conventional, integrated and organic farming on ascorbic acid (AA) and α,β-carotene levels of new carrot cultivars Cortina F1 and Afalon F1 was investigated and their metabolomic profiles were measured by direct analysis in real time ion source coupled with a high-resolution mass spectrometer (DART-HRMS). Cortina and Afalon exhibited high levels of AA and total carotenes under all agroecological conditions tested that fluctuated in broad ranges of 215–539 and 173–456 mg AA.kg−1 dry biomass and 1069–2165 and 1683–2165 mg carotene.kg−1 dry biomass, respectively. The ratio of β- to α-carotene in both cultivars was about 1.3. The most important variable for the PCA and the partial least squares discriminant analysis (PLS-DA) models for ethyl acetate extracts measured in positive and negative ionization mode was 6-methoxymellein (6-MM). Total carotene content and 6-MM levels were higher in the organic carrot compared to the conventional one and were correlated with a higher level of spontaneous infection. Other important compounds identified were sitosterol, hexose and various organic acids including antioxidant ferulic and coumaric acids. The findings allow comparison of metabolomic profiles and the AA and carotene contents of both cultivars with those of other commercially used carrots.
Numerical Wear Analysis of a PLA-Made Spur Gear Pair as a Function of Friction Coefficient and Temperature
Authors: Fekete Gusztáv
Published: April 2021

Polylactic acid (PLA)-made machine elements exhibit easy machining, biodegradability, and excellent mechanical properties. However, enhancing their wear resistance is still a crucial engineering point, which may be achieved by altering (lowering) their coefficient of friction (CoF). Therefore, the first aim of this paper is to analyze how wear is affected by the alteration of CoF. The second aim is connected to the fact that PLA is sensitive to heat, which also limits its applicability. Accordingly, the next goal is to explore the effect of temperature on wear propagation. This study answers these questions by means of multibody dynamics simulations of a PLA-made spur gear pair. Simulations were carried out under constant torque, while the CoF and the temperature were varied in a normal operation domain (CoF: 0.1–0.05, T = 20–30 °C). The results showed that the wear volume gradually began to decline at approximately 0.085 CoF, whilst convergence to steady-state wear could be observed at 0.05 CoF. In conclusion, alteration of the CoF can lower wear by 35%, in this specific domain, while even a 5 °C rise in temperature causes 40% wear progression. The feasibility of the numerical procedure was validated by comparing numerically and experimentally obtained wear–torque results.
Non-invasive Optical Technical Identification of Red Pigments on Chinese Paper Notes
Authors: Ren Jie , Gao Cunjin , Wang Jigang , Shen Yang , Shi Jilong , Liu Quanxiao , Chen Wei
Published: April 2021

Red pigments with bright colors were widely used in ancient Chinese painted pottery, books, antiques, calligraphy, and paintings. Herein, red pigments of traditional paper notes were investigated by non-invasive optical technology in order to enrich the Chinese historical pigments knowledge base. The results of laser Raman spectroscopy tests on five paper notes clearly identified the inorganic mineral pigments including ocher and cinnabar. Infrared spectroscopy measurements indicated that an artificial synthetic magenta was employed as the organic pigment. Inorganic and organic red pigments were applied together on the same samples 2 and 5 which can be speculated to serve an anti-counterfeiting function. In addition, SEM-EDS analysis of sample 5 clearly showed that the red pigment was composed of lead oxides and ZnS was added as color modulator. Combined with the abovementioned non-invasive techniques, analysis of printed pigments can provide a feasible method to authenticate and conserve paper notes.
Effects of Oxygen Partial Pressure on Wood-Based Activated Carbon Treated with Vacuum Ultraviolet Light
Authors: Takahashi Nana , Kuwae Hiroyuki , Maeda Seren , Kawamura Masahiro , Tezuka Ami , Shoji Shuichi , Mizuno Jun
Published: April 2021

This study uses vacuum ultraviolet (VUV) light with a wavelength of 172 nm as a surface treatment to enhance the adsorption capacity of wood-based activated carbon (AC). The AC surface treatment is performed under three O2 partial pressure conditions—5.0 × 104 Pa, where ozone (O3) effects dominate; 6.3 × 10−6 Pa, where VUV effects dominate; and 1.9 × 103 Pa for a balanced condition. For the O3-dominant condition, only graphene edge defects are etched (no aromatic carbon bonds are etched), resulting in increased surface roughness. When the VUV effects dominate, aromatic carbon bonds are cleaved, which then reacted with O2 or water adsorbed inside the pores. This increased both the number and size of the mesopores. Under the balanced conditions, the water adsorption capacity was enhanced by 45.5%, which is higher than that obtained before VUV exposure or with VUV under other conditions. This is because the surface roughness increased, as well as the pore sizes and numbers under the balanced condition. These results indicate that we can control VUV-based AC surface treatments via O2 partial pressure.
Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens
Authors: Mairiang Dumrong , Songjaeng Adisak , Hansuealueang Prachya , Malila Yuwares , Lertsethtakarn Paphavee , Silapong Sasikorn , Poolpanichupatam Yongyuth , Klungthong Chonticha , Chin-Inmanu Kwanrutai , Thiemmeca Somchai , Tangthawornchaikul Nattaya , Sriraksa Kanokwan , Limpitikul Wannee , Vasanawathana Sirijitt , Ellison Damon W. , Malasit Prida , Suriyaphol Prapat , Avirutnan Panisadee
Published: April 2021

Detection and quantification of viruses in laboratory and clinical samples are standard assays in dengue virus (DENV) studies. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) is considered to be the standard for DENV detection and quantification due to its high sensitivity. However, qRT-PCR offers only quantification relative to a standard curve and consists of several “in-house” components resulting in interlaboratory variations. We developed and optimized a protocol for applying one-step RT-droplet digital PCR (RT-ddPCR) for DENV detection and quantification. The lower limit of detection (LLOD95) and the lower limit of quantification (LLOQ) for RT-ddPCR were estimated to be 1.851 log10-copies/reaction and 2.337 log10-copies/reaction, respectively. The sensitivity of RT-ddPCR was found to be superior to qRT-PCR (94.87% vs. 90.38%, p = 0.039) while no false positives were detected. Quantification of DENV in clinical samples was independently performed in three laboratories showing interlaboratory variations with biases <0.5 log10-copies/mL. The RT-ddPCR protocol presented here could help harmonize DENV quantification results and improve findings in the field such as identifying a DENV titer threshold correlating with disease severity.
Universal Design for Learning: The More, the Better?
Authors: Roski Marvin , Walkowiak Malte , Nehring Andreas
Published: April 2021

An experimental study investigated the effects of applying principles of the Universal Design for Learning (UDL). Focusing on epistemic beliefs (EBs) in inclusive science classes, we compared four groups who worked with learning environments based more or less on UDL principles and filled out an original version of a widely used EBs questionnaire or an adapted version using the Universal Design for Assessment (UDA). Based on measurement invariance analyses, a multiple indicator, and multiple cause (MIMIC) approach as well as multi-group panel models, the results do not support an outperformance of the extensive UDL environment. Moreover, the UDA-based questionnaire appears to be more adequately suited for detecting learning gains in an inclusive setting. The results emphasize how important it is to carefully adopt and introduce the UDL principles for learning and to care about test accessibility when conducting quantitative research in inclusive settings.
Employees’ Trust in Artificial Intelligence in Companies: The Case of Energy and Chemical Industries in Poland
Authors: Łapińska Justyna , Escher Iwona , Górka Joanna , Sudolska Agata , Brzustewicz Paweł
Published: April 2021

The use of artificial intelligence (AI) in companies is advancing rapidly. Consequently, multidisciplinary research on AI in business has developed dramatically during the last decade, moving from the focus on technological objectives towards an interest in human users’ perspective. In this article, we investigate the notion of employees’ trust in AI at the workplace (in the company), following a human-centered approach that considers AI integration in business from the employees’ perspective, taking into account the elements that facilitate human trust in AI. While employees’ trust in AI at the workplace seems critical, so far, few studies have systematically investigated its determinants. Therefore, this study is an attempt to fill the existing research gap. The research objective of the article is to examine links between employees’ trust in AI in the company and three other latent variables (general trust in technology, intra-organizational trust, and individual competence trust). A quantitative study conducted on a sample of 428 employees from companies of the energy and chemical industries in Poland allowed the hypotheses to be verified. The hypotheses were tested using structural equation modeling (SEM). The results indicate the existence of a positive relationship between general trust in technology and employees’ trust in AI in the company as well as between intra-organizational trust and employees’ trust in AI in the company in the surveyed firms.
Improving Power Quality by a Four-Wire Shunt Active Power Filter: A Case Study
Authors: Popescu Mihaela , Bitoleanu Alexandru , Linca Mihaita , Suru Constantin Vlad
Published: April 2021

This paper presents the use of a three-phase four-wire shunt active power filter to improve the power quality in the Department of Industrial Electronics of a large enterprise from Romania. The specificity is given by the predominant existence of single-phase consumers (such as personal computers, printers, lighting and AC equipment). In order to identify the power quality indicators and ways to improve them, an A-class analyzer was used to record the electrical quantities and energy parameters in the point of common coupling (PCC) with the nonlinear loads for 27 h. The analysis shows that, in order to improve the power quality in PCC, three goals must be achieved: the compensation of the distortion power, the compensation of the reactive power and the compensation of the load unbalance. By using the conceived three-leg shunt active power filter, controlled through the indirect current control method in an original variant, the power quality at the supply side is very much improved. In the proposed control algorithm, the prescribed active current is obtained as a sum of the loss current provided by the DC voltage and the equivalent active current of the unbalanced load. The performance associated with each objective of the compensation is presented and analyzed. The results show that all the power quality indicators meet the specific standards and regulations and prove the validity of the proposed solution.
Genetic Diversity and Association Analysis among Germplasms of Diospyros kaki in Zhejiang Province Based on SSR Markers
Authors: Xu Yang , Cheng Wenqiang , Xiong Chunyan , Jiang Xibing , Wu Kaiyun , Gong Bangchu
Published: April 2021

In subtropical to temperate regions, persimmon (Diospyros kaki Thunb.) is an economically important fruit crop cultivated for its edible fruits. Persimmons are distributed abundantly and widely in Zhejiang Province, representing a valuable resource for the breeding of new cultivars and studying the origin and evolution of persimmon. In this study, we elucidated the genetic structures and diversity patterns of 179 persimmon germplasms from 16 different ecologic populations in Zhejiang Province based on the analysis of 17 SSR markers. The results show that there was a medium degree of genetic diversity for persimmon found in Zhejiang Province. With the exception of the Tiantai Mountain and Xin’an River populations, we found extensive gene exchange had occurred among the other populations. The 179 D. kaki germplasms from the 16 populations could be separated into three distinct clusters (I, II, and III) with a higher mean pairwise genetic differentiation index (FST) (0.2714). Nearly all samples of Cluster-I were distributed inland. Cluster-II and Cluster-III contained samples that were widely distributed throughout Zhejiang Province including all samples from the coastal populations and the Northeast Plain populations. In addition, we performed association mapping with nine traits (fruit crude fiber content, fruit calcium content, fruit water content, fruit longitudinal diameter, fruit aspect ratio, seed width, seed length, leaf aspect ratio, and number of lateral veins) using these markers. This led to the identification of 13 significant marker–trait associations (MTAs; p < 0.00044, 0.1/228) using a general linear model, of which, six MTAs with a correlation coefficient (R2) >10% were consistently represented in the general linear model with p < 0.00044 in the two models. The genetic structures and diversity patterns of the persimmon germplasms revealed in this study will provide a reference for the efficient conservation and further utilization of persimmon germplasms. The MTAs identified in this study will be useful for future marker-assisted breeding of persimmon.
The Effect of Informal Tourist Trails on the Abiotic Conditions and Floristic Composition of Deciduous Forest Undergrowth in an Urban Area
Authors: Kostrakiewicz-Gierałt Kinga , Pliszko Artur , Gmyrek Katarzyna
Published: April 2021

The study aimed to assess the effect of informal tourist trails on the abiotic conditions and floristic composition of deciduous forest undergrowth in an urban area. The study was carried out in the Wolski Forest in Kraków (Poland) in 2020 using a total of 400 plots (1 m × 1m) situated in close (CL) and further (FU) vicinity of informal (<50 cm in width) and formal (≥150 cm in width) tourist trails in the forest interior and forest edge sites.Agreater content of N-NH4, P, and K occurred in the forest interior sites, while greater light intensity, species number, total plant cover, and stem height were found in the forest edge sites. Greater light intensity and a higher percentage of total and damaged plant cover were recorded in CLplots, while a greater height of plants was seen in FUplots. The number of species was higher in plots located along informal trails than in plots along formal trails. The dominance of therophytes and autochorous species along informal trails in the forest interior sites might be a result of successful diaspore dispersal by people walking, while the substantial share of zoochorous species along formal trails suggests their dissemination by dogs accompanying visitors. The dominance of forest and ruderal taxa in the forest interior sites suggests unfavourable conditions for the germination of meadow and grassland species. The higher cover-abundance of alien plants in CL plots along informal trails and FU plots along formal traits suggests that alien plants growing near the formal trails are more exposed to mechanical damage.
Crown Structure Explains the Discrepancy in Leaf Phenology Metrics Derived from Ground- and UAV-Based Observations in a Japanese Cool Temperate Deciduous Forest
Authors: Budianti Noviana , Mizunaga Hiromi , Iio Atsuhiro
Published: April 2021

Unmanned aerial vehicles (UAV) provide a new platform for monitoring crown-level leaf phenology due to the ability to cover a vast area while offering branch-level image resolution. However, below-crown vegetation, e.g., understory vegetation, subcanopy trees, and the branches of neighboring trees, along with the multi-layered structure of the target crown may significantly reduce the accuracy of UAV-based estimates of crown leaf phenology. To test this hypothesis, we compared UAV-derived crown leaf phenology results against those based on ground observations at the individual tree scale for 19 deciduous broad-leaved species (55 individuals in total) characterized by different crown structures. The mean crown-level green chromatic coordinate derived from UAV images poorly explained inter- and intra-species variations in spring leaf phenology, most probably due to the consistently early leaf emergence in the below-crown vegetation. The start dates for leaf expansion and end dates for leaf falling could be estimated with an accuracy of <1-week when the influence of below-crown vegetation was removed from the UAV images through visual interpretation. However, a large discrepancy between the phenological metrics derived from UAV images and ground observations was still found for the end date of leaf expansion (EOE) and start date of leaf falling (SOF). Bayesian modeling revealed that the discrepancy for EOE increased as crown length and volume increased. The crown structure was not found to contribute to the discrepancy in SOF value. Our study provides evidence that crown structure is a pivotal factor to consider when using UAV photography to reliably estimate crown leaf phenology at the individual tree-scale.
Miskâsowin—Returning to the Body, Remembering What Keeps Us Alive
Authors: Clark Moe , Aviles-Betel Kenna , Richardson Catherine , Allouche Zeina
Published: April 2021

The nêhiyawêwin (Plains Cree language) Cree word, miskâsowin, relates to the sacred teachings of Treaty Elders of Saskatchewan as a concept pertaining to wellness of “finding one’s sense of belonging”—a process integral in the aftermath of colonial disruption. Métis educator and performance artist Moe Clark offers an approach to healing and well-being, which is imparted through movement, flux and through musical and performance-based engagement. Moe works with tools of embodiment in performance and circle work contexts, including song creation, collaborative performance, participatory youth expression and land-based projects as healing art. She shares her process for re-animating these relationships to land, human kin, and other-than-human kin through breath-work, creative practice and relationality as part of a path to wholeness. The authors document Moe’s approach to supporting the identity, growth, healing and transformation of others.
A Hypomorphic Mutant of PHD Domain Protein Male Meiocytes Death 1
Authors: Liu Bing , Jin Chunlian , De Storme Nico , Schotte Sébastien , Schindfessel Cédric , De Meyer Tim , Geelen Danny
Published: April 2021

Meiosis drives reciprocal genetic exchanges and produces gametes with halved chromosome number, which is important for the genetic diversity, plant viability, and ploidy consistency of flowering plants. Alterations in chromosome dynamics and/or cytokinesis during meiosis may lead to meiotic restitution and the formation of unreduced microspores. In this study, we isolated an Arabidopsis mutant male meiotic restitution 1 (mmr1), which produces a small subpopulation of diploid or polyploid pollen grains. Cytological analysis revealed that mmr1 produces dyads, triads, and monads indicative of male meiotic restitution. Both homologous chromosomes and sister chromatids in mmr1 are separated normally, but chromosome condensation at metaphase I is slightly affected. The mmr1 mutant displayed incomplete meiotic cytokinesis. Supportively, immunostaining of the microtubular cytoskeleton showed that the spindle organization at anaphase II and mini-phragmoplast formation at telophase II are aberrant. The causative mutation in mmr1 was mapped to chromosome 1 at the chromatin regulator Male Meiocyte Death 1 (MMD1/DUET) locus. mmr1 contains a C-to-T transition at the third exon of MMD1/DUET at the genomic position 2168 bp from the start codon, which causes an amino acid change G618D that locates in the conserved PHD-finger domain of histone binding proteins. The F1 progenies of mmr1 crossing with knockout mmd1/duet mutant exhibited same meiotic defects and similar meiotic restitution rate as mmr1. Taken together, we here report a hypomorphic mmd1/duet allele that typically shows defects in microtubule organization and cytokinesis.
Impact of COVID-19 on Out-of-Hospital Cardiac Arrest in Singapore
Authors: Lim Shir Lynn , Shahidah Nur , Saffari Seyed Ehsan , Ng Qin Xiang , Ho Andrew Fu Wah , Leong Benjamin Sieu-Hon , Arulanandam Shalini , Siddiqui Fahad Javaid , Ong Marcus Eng Hock
Published: April 2021

This study aimed to evaluate the impact of the Coronavirus Disease 2019 (COVID-19) pandemic on out-of-hospital cardiac arrest (OHCA) in Singapore. We used data from the Singapore Civil Defence Force to compare the incidence, characteristics and outcomes of all Emergency Medical Services (EMS)-attended adult OHCA during the pandemic (January–May 2020) and pre-pandemic (January–May 2018 and 2019) periods. Pre-hospital return of spontaneous circulation (ROSC) was the primary outcome. Binary logistic regression was used to calculate the adjusted odds ratios (aOR) for the characteristics of OHCA. Of the 3893 OHCA patients (median age 72 years, 63.7% males), 1400 occurred during the pandemic period and 2493 during the pre-pandemic period. Compared with the pre-pandemic period, OHCAs during the pandemic period more likely occurred at home (aOR: 1.48; 95% CI: 1.24–1.75) and were witnessed (aOR: 1.71; 95% CI: 1.49–1.97). They received less bystander CPR (aOR: 0.70; 95% CI: 0.61–0.81) despite 65% of witnessed arrests by a family member, and waited longer for EMS (OR ≥ 10 min: 1.71, 95% CI 1.46–2.00). Pre-hospital ROSC was less likely during the pandemic period (aOR: 0.67; 95% CI: 0.53–0.84). The pandemic saw increased OHCA incidence and worse outcomes in Singapore, likely indirect effects of COVID-19.
COVID-19 Pandemic Is Associated with an Adverse Impact on Burnout and Mood Disorder in Healthcare Professionals
Authors: Lin Yu-Yin , Pan Yu-An , Hsieh Yi-Ling , Hsieh Meng-Hsuan , Chuang Yun-Shiuan , Hsu Hsiu-Yi , Huang Ya-Hsiu , Hsu Chia-En , Cheng Yi-Chen , Cho Shih-Feng , Wang Chao-Ling
Published: April 2021

The coronavirus disease 2019 (COVID-19) pandemic results in a profound physical and mental burden on healthcare professionals. This study aims to evaluate burnout status and mood disorder of healthcare workers during this period. An online questionnaire was voluntarily answered by eligible adult employees in a COVID-19 specialized medical center. The major analysis included the burnout status and mood disorder. Factors related to more severe mood disorder were also identified. A total of 2029 participants completed the questionnaire. There were 901 (44.4%) and 923 (45.5%) participants with moderate to severe personal and work-related burnout status, respectively. Nurses working in the emergency room (ER), intensive care unit (ICU)/isolation wards, and general wards, as well as those with patient contact, had significantly higher scores for personal burnout, work-related burnout, and mood disorder. This investigation identified 271 participants (13.35%) with moderate to severe mood disorder linked to higher personal/work-related burnout scores and a more advanced burnout status. Univariate analysis revealed that nurses working in the ER and ICU/isolation wards were associated with moderate to severe mood disorder risk factors. Multivariate analysis demonstrated that working in the ER (OR, 2.81; 95% CI, 1.14–6.90) was the only independent risk factor. More rest, perquisites, and an adequate supply of personal protection equipment were the most desired assistance from the hospital. Compared with the non-pandemic period (2019), employees working during the COVID-19 pandemic (2020) have higher burnout scores and percentages of severe burnout. In conclusion, this study suggests that the COVID-19 pandemic has had an adverse impact on healthcare professionals. Adequate measures should be adopted as early as possible to support the healthcare system.
High-Intensity Interval Exercise Performance and Short-Term Metabolic Responses to Overnight-Fasted Acute-Partial Sleep Deprivation
Authors: Papadakis Zacharias , Forsse Jeffrey S. , Stamatis Andreas
Published: April 2021

People practicing high-intensity interval exercise (HIIE) fasted during the morning hours under a lack of sleep. Such a habit may jeopardize the health benefits related to HIIE and adequate sleep. Fifteen habitually good sleeper males (age 31.1 ± 5.3 SD year) completed on a treadmill two isocaloric (500 kcal) HIIE sessions (3:2 min work:rest) averaged at 70% VO2reserve after 9–9.5 h of reference sleep exercise (RSE) and after 3–3.5 h of acute-partial sleep deprivation exercise (SSE). Diet and sleep patterns were controlled both 1 week prior and 2 days leading up to RSE and SSE. HIIE related performance and substrate utilization data were obtained from the continuous analysis of respiratory gases. Data were analyzed using repeated measures ANOVA with the baseline maximum oxygen uptake (VO2max) and body fat percentage (BF%) as covariates at p < 0.05. No difference was observed in VO2max, time to complete the HIIE, VE, RER, CHO%, and FAT% utilization during the experimental conditions. Whether attaining an adequate amount of sleep or not, the fasted HIIE performance and metabolism were not affected. We propose to practice the fasted HIIE under adequate sleep to receive the pleiotropic beneficial effects of sleep to the human body.
Examining the Role of Acculturation in E-Cigarette Use among U.S. Immigrant Adults
Authors: Azagba Sunday , Shan Lingpeng
Published: April 2021

Evidence suggests that as immigrants’ length of residence in the host country increases, they may integrate their behavior and norms to align with the new community’s cultural norms. The current study examined e-cigarette use among immigrants in the U.S., and whether the length of residence in the U.S. is associated with e-cigarette use among immigrants compared to the native-born population. Data were drawn from the 2014/15 and 2018/19 Tobacco Use Supplement to the Current Population Survey. Multivariable logistic regression was used to compare differences in e-cigarette use between native-born populations and immigrants, when immigrants’ length of residence in the U.S. was considered. Among immigrants, the prevalence of ever and current e-cigarette use increased significantly from 2.5% and 0.5% in 2014/2015 to 3.2% and 0.8% in 2018/2019, respectively. Multivariable analysis showed that immigrants had significantly lower odds of ever e-cigarette use compared to the mainland-born citizen (0–5 years in the U.S., adjusted Odds Ratio (aOR) 0.57, 95% Confidence Interval (CI) 0.46–0.69; 6–10 years, aOR 0.51, 95% CI 0.41–0.63; 11–20 years, aOR 0.45, 95% CI 0.39–0.53; 20+years, aOR 0.68, 95% CI 0.62–0.76). Similar results were found for current e-cigarette use, with immigrants being less likely to be current users. Findings that e-cigarette use among all immigrants—regardless of years living in the U.S.—was consistently lower than among the native-born population run contrary to the notion that as length of stay increases, health behaviors between immigrants and native populations of the host country become similar.
Transition of Hypertriglyceridemic-Waist Phenotypes and the Risk of Type 2 Diabetes Mellitus among Middle-Aged and Older Chinese: A National Cohort Study
Authors: Chen Ge , Yi Qian , Hou Leying , Peng Shenghan , Fan Mengya , Song Peige , Zhu Yimin
Published: April 2021

The rapid economic growth and nutritional changes in China have brought an increased burden of type 2 diabetes mellitus (T2DM). This study aimed to assess the effects of hypertriglyceridemic-waist (HTW) and its dynamic transitions on incident T2DM among middle-aged and older Chinese. Data were extracted from the China Health and Retirement Longitudinal Study (CHARLS). Participants were classified into three HTW phenotypes, namely NTNW (normal triglyceride (TG) and waist circumference (WC)), NTEW/ETNW (normal TG and enlarged WC, or elevated TG and normal WC) and ETEW (elevated TG and enlarged WC). Multivariable Cox frailty models were used to assess the associations of HTW phenotypes and their transitions over time with the risk of T2DM. A total of 7397 subjects without T2DM were included, of which 849 developed T2DM during 2011–2018. Compared with individuals with NTNW, people in the NTEW/ETNW group and ETEW group were at a significantly higher risk of T2DM (HRNTEW/ETNW = 1.28, 95% CI: 1.06–1.54 and HRETEW = 1.61, 95% CI: 1.26–2.06). For subjects with NTNW at baseline, the risk of developing T2DM increased by 38% and 83% if their metabolic status changed to NTEW/ETNW and ETEW, respectively. For subjects with NTEW/ETNW, the risk of T2DM decreased by 33% when their metabolic status changed to normal (NTNW); but the risk increased by 49% if the status became more serious (ETEW). NTEW/ETNW, ETEW and their transitions to adverse states were risk factors for T2DM.
A Pilot Evaluation of mHealth App Accessibility for Three Top-Rated Weight Management Apps by People with Disabilities
Authors: Radcliffe Erin , Lippincott Ben , Anderson Raeda , Jones Mike
Published: April 2021

Growing evidence demonstrates that people with disabilities face more challenges in accessing healthcare and wellness resources, compared to non-disabled populations. As mobile applications focused on health and wellness (mHealth apps) become prevalent, it is important that people with disabilities can access and use mHealth apps. At present, there is no source of unified information about the accessibility and usability of mHealth apps for people with disabilities. We set out to create such a source, establishing a systematic approach for evaluating app accessibility. Our goal was to develop a simple, replicable app evaluation process to generate useful information for people with disabilities (to aid suitable app selection) and app developers (to improve app accessibility and usability). We collected data using two existing assessment instruments to test three top-rated weight management apps with nine users representing three disability groups: vision, dexterity, and cognitive impairment. Participants with visual impairments reported the lowest accessibility ratings, most challenges, and least tolerance for issues. Participants with dexterity impairments experienced significant accessibility-related difficulties. Participants with cognitive impairments experienced mild difficulties and higher tolerances for issues. Our pilot protocol will be applied to test mHealth apps and populate a “curation” website to assist consumers in selecting mHealth apps.
Precarious Essential Work, Immigrant Dairy Farmworkers, and Occupational Health Experiences in Vermont
Authors: Panikkar Bindu , Barrett Mary-Kate
Published: April 2021

Migrant dairy workers in Vermont face a wide range of occupational and health hazards at work. This research examines the environmental risks, occupational health hazards, and health outcomes experienced by migrant dairy farm workers in Vermont. This research draws on a triangulation of sources including analysis of data—surveys and interviews with migrant dairy farmworkers gathered by the organization Migrant Justice since 2015 as well as relevant key informant interviews with community organizations across the state to characterize the occupational health experiences of migrant dairy workers in Vermont. Our results show that Vermont migrant dairy farmworkers received poor health and safety training and lacked sufficient protective gear. Over three quarters of the respondents reported experiencing harm from chemical and biological risks. Close to half the survey respondents reported headaches, itchy eyes and cough; a quarter reported breathing difficulties; three fourths reported being hurt by animal-related risks. These exposures and existing health concerns are avoidable. Migrant workers require better social representation and advocates to negotiate better work-related protection and training, access to health services, and social welfare to ensure their health and safety.
Could tDCS Be a Potential Performance-Enhancing Tool for Acute Neurocognitive Modulation in eSports? A Perspective Review
Authors: Machado Sergio , Travassos Bruno , Teixeira Diogo S. , Rodrigues Filipe , Cid Luis , Monteiro Diogo
Published: April 2021

Competitive sports involve physical and cognitive skills. In traditional sports, there is a greater dependence on the development and performance of both motor and cognitive skills, unlike electronic sports (eSports), which depend much more on neurocognitive skills for success. However, little is known about neurocognitive functions and effective strategies designed to develop and optimize neurocognitive performance in eSports athletes. One such strategy is transcranial direct current stimulation (tDCS), characterized as a weak electric current applied on the scalp to induce prolonged changes in cortical excitability. Therefore, our objective is to propose anodal (a)-tDCS as a performance-enhancing tool for neurocognitive functions in eSports. In this manuscript, we discussed the neurocognitive processes that underlie exceptionally skilled performances in eSports and how tDCS could be used for acute modulation of these processes in eSports. Based on the results from tDCS studies in healthy people, professional athletes, and video game players, it seems that tDCS is applied over the left dorsolateral prefrontal cortex (DLPFC) as a potential performance-enhancing tool for neurocognition in eSports.
Midday Nap Duration and Hypertension among Middle-Aged and Older Chinese Adults: A Nationwide Retrospective Cohort Study
Authors: Fu Jialin , Zhang Xinge , Moore Justin B. , Wang Bowen , Li Rui
Published: April 2021

The goal of this study was to investigate the associations of midday nap duration and change in midday nap duration with hypertension in a retrospective cohort using a nationwide representative sample of middle-aged and older Chinese adults. Data were obtained from the China Health and Retirement Longitudinal Study (CHARLS) database during 2011–2015. Information on midday nap duration was collected via a self-reported questionnaire and blood pressure was objectively measured. Hazard ratios (HR) with 95% confidence interval (CI) were estimated using Cox proportional hazards regression models to quantify the associations. A sample of 5729 Chinese adults (≥45 years old) were included in the longitudinal analysis. Relative to non-nappers, participants who napping for ≥90 min/day was associated with significantly larger HR for hypertension at four-year follow-up (HR = 1.18, 95% CI = 1.01–1.40, p = 0.048). Compared with people who napped ≥90 min/day both at baseline (2011) and follow-up (2013), hypertension risk at four-year follow-up declined in individuals whose midday nap durations decreased in the 2-year study period from ≥ 90 min/day to 1–59 min/day (HR = 0.59, 95% CI = 0.36–0.97, p = 0.037) and 60–89 min/day (HR = 0.68, 95% CI = 0.47–0.99, p = 0.044). Among middle-aged and older Chinese adults, relative to non-nappers, people who had longer midday nap duration (≥90 min/day) were associated with significantly larger HR for hypertension and decreased napping duration may confer benefit for hypertension prevention.
Analyzing Gender Differences in Factors Affecting Depression among Multicultural Adolescents in South Korea: A Cross-Sectional Study
Authors: Lee Eun Jee , Jeong Sookyung
Published: April 2021

Suicide is the topmost cause of death among adolescents in South Korea and is deeply related to depression. This study aimed to identify gender differences in the factors affecting depression among multicultural adolescents. This study is a secondary analysis using data from a national survey, the Multicultural Adolescents Panel Study (MAPS) conducted in 2017. The participants were 1160 multicultural adolescents ranging from 15 to 18 years, living in Korea, and whose fathers were Koreans and mothers were foreigners. The results showed that depression scores were higher for females (18.35) than males (16.38, t = 6.42, p < 0.001). In total, seven factors affected depression among male multicultural adolescents’ and the model explained 50.5% of the total variance (F = 77.99, p < 0.001), while four factors affected female multicultural adolescents’ depression, and the model explained 51.4% of the total variance (F = 100.02, p < 0.001). Significant gender differences were found in factors that influence depression among multicultural adolescents. Therefore, depression prevention programs for multicultural adolescents need to vary according to gender. Additionally, these programs should target families and teachers of multicultural adolescents as well.