bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute Administration of Metformin Protects Against Neuronal Apoptosis Induced by Cerebral Ischemia-Reperfusion Injury via Regulation of the AMPK/CREB/BDNF Pathway
Authors: Liu Ke , Li Lulu , Liu Zhijun , Li Gang , Wu Yanqing , Jiang Xingjun , Wang Mengdie , Chang Yanmin , Jiang Tingting , Luo Jianheng , Zhu Jiahui , Li Hongge , Wang Yong
Published: April 2022

Metformin is a first-line anti-diabetic agent with a powerful hypoglycemic effect. Several studies have reported that metformin can improve the prognosis of stroke patients and that this effect is independent of its hypoglycemic effect; however, the specific mechanism remains unclear. In this research, we explored the effect and specific mechanism of metformin in cerebral ischemia-reperfusion (I/R) injury by constructing a transient middle cerebral artery occlusion model in vivo and a glucose and oxygen deprivation/reoxygenation (OGD/R) model in vitro. The results of the in vivo experiments showed that acute treatment with low-dose metformin (10 mg/kg) ameliorated cerebral edema, reduced the cerebral infarction volume, improved the neurological deficit score, and ameliorated neuronal apoptosis in the ischemic penumbra. Moreover, metformin up-regulated the brain-derived neurotrophic factor (BDNF) expression and increased phosphorylation levels of AMP-activated protein kinase (AMPK) and cAMP-response element binding protein (CREB) in the ischemia penumbra. Nevertheless, the above-mentioned effects of metformin were reversed by Compound C. The results of the in vitro experiments showed that low metformin concentrations (20 μM) could reduce apoptosis of human umbilical vein endothelial cells (HUVECs) under OGD/R conditions and promote cell proliferation. Moreover, metformin could further promote BDNF expression and release in HUVECs under OGD/R conditions via the AMPK/CREB pathway. The Transwell chamber assay showed that HUVECs treated with metformin could reduce apoptosis of SH-SY5Y cells under OGD/R conditions and this effect could be partially reversed by transfection of BDNF siRNA in HUVECs. In summary, our results suggest that metformin upregulates the level of BDNF in the cerebral ischemic penumbra via the AMPK/CREB pathway, thereby playing a protective effect in cerebral I/R injury.
Chinese Herbal Medicine (Yiqi-Yangyin-Jiedu Decoction) Combined With Osimertinib as First-Line Treatment in EGFR Mutation-Positive Advanced Non-Small-Cell Lung Cancer (CATLA-2): A Study Protocol for a Double-Blind Randomized Controlled Trial
Authors: Yao Jialin , Lu Yan , Jiao Lijing , Bi Ling , Yang Wenxiao , Su Lingzi , Shi Jun , Wang Zhe , Gong Yabin , Xu Ling
Published: April 2022

Background: Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (EGFR-TKIs) significantly improve the prognosis of non-small cell lung cancer (NSCLC) with EGFR mutation-positive. Although third-generation EGFR-TKI osimertinib is demonstrated with superior efficacy compared with first-generation EGFR-TKIs, acquired resistance to EGFR-TKIs remains the bottleneck. The Chinese herbal medicine (CHM) Yiqi-Yangyin-Jiedu decoction (YYJD) has been shown to delay acquired resistance to first-generation EGFR-TKIs in the CATLA study, but there is no high-level evidence for its effect when combined with osimertinib. This trial aims to evaluate the efficacy and safety of YYJD combined with osimertinib as first-line treatment in EGFR mutation-positive advanced NSCLC.

Methods: This is a double-blind, multi-center, randomized controlled trial conducted in eight hospitals in China. A total of 314 participants will be randomly assigned to the osimertinib plus YYJD group (O+YYJD) or the osimertinib plus placebo group (O+placebo). Treatment will last until disease progression or death. Patients diagnosed with advanced NSCLC harboring EGFR Ex19del or L858R will be enrolled if they are ready to take osimertinib as first-line treatment, aged 18–74 years old, and provide signed informed consent. The primary outcome is progression-free survival (PFS). The secondary outcomes include a comparison of overall survival (OS), objective response rate (ORR), disease control rate (DCR), and quality of life (QoL). The analysis will be based on intention-to-treat and per-protocol subject analysis principles.

Discussion: The goal of this trial is to evaluate the efficacy and safety of YYJD when added to osimertinib as first-line treatment in EGFR mutation-positive advanced NSCLC.
Hydralazine Associated With Reduced Therapeutic Phlebotomy Frequency in a Nationwide Cohort Study: Real-World Effectiveness for Drug Repurposing
Authors: Lin Wei-Zhi , Chung Chi-Hsiang , Shaiu Chia-Yang , Yang Bing-Heng , Chien Wu-Chien
Published: April 2022

Background: Therapeutic phlebotomy, known as scheduled bloodletting, has been the main method for managing erythrocytosis symptoms and thrombocytosis-associated complications in various blood disorders. One of the major indications for phlebotomy is polycythemia vera (PV). The main goal of current treatment strategies for patients who require phlebotomy is to prevent thrombohemorrhagic complications rather than to prolong survival or lessen the risk of myelofibrotic or leukemic progression. Additional cytoreductive therapy is recommended for high-risk PV, for which the common first-line drug is hydroxyurea. However, recent evidence suggests that phlebotomy may not reduce the risk of thrombosis in patients with PV. Further evidence suggests that patients with PV treated with hydroxyurea who require three or more phlebotomy procedures per year have a higher risk of thrombotic complications.

Methods: We hypothesized that a drug-repurposing strategy of utilizing antineoplastic drugs for patients who require phlebotomy would result in greater benefits than would phlebotomy. The antihypertensive hydralazine and the anticonvulsant valproate, which have both been reported to have antineoplastic activity that mimics cytoreductive agents, were selected as candidates for the drug-repositioning strategy in a retrospective cohort study. We measured the hazard ratios (HR) and the frequencies of phlebotomy in patients with prescriptions for hydralazine or valproate or the two drugs in combination by using data from Taiwan’s National Health Insurance Research Database from 2000 to 2015 (n = 1,936,512).

Results: The HRs of undergoing phlebotomy in groups with hydralazine, valproate, and combination hydralazine–valproate prescriptions were reduced to 0.729 (p = 0.047), 0.887 (p = 0.196), and 0.621 (p = 0.022), respectively. The frequency of undergoing phlebotomy decreased from 2.27 to 1.99, 2.01, and 1.86 per person-year (p = 0.015), respectively. However, no significant differences were observed for the hydralazine group or the hydralazine–valproate combination group.

Conclusion: Whether a repurposed drug can serve as a cytoreductive agent for patients who require phlebotomy depends on its risk–benefit balance. We suggest that hydralazine, instead of the hydralazine–valproate combination, is a reasonable alternative for patients who require regular phlebotomy.
Long Non-Coding RNA NR-133666 Promotes the Proliferation and Migration of Fibroblast-Like Synoviocytes Through Regulating the miR-133c/MAPK1 Axis
Authors: Zhang Nanwen , Zheng Ningning , Luo Dunxiong , Lin Duoduo , Que Wenzhong , Wang He , Huang Qiuping , Yang Juhua , Ye Jian , Chen Xiaole
Published: April 2022

Long non-coding RNA (lncRNA) is involved in the regulation of rheumatoid arthritis (RA) and many other diseases. In this study, a new lncRNA, NR-133666, was identified to be highly expressed in the adjuvant-induced arthritis rat model using the Agilent lncRNA microarray assay. qRT-PCR verified that NR-133666 was upregulated in fibroblast-like synoviocyte of a collagen-induced arthritis (CIA) rat model. Fluorescence in situ hybridization analysis showed that NR-133666 is mainly expressed in the cytoplasm of collagen-induced arthritis FLS. MTT assay and EdU staining results showed that the proliferation of CIA FLS was inhibited after NR-133666 was knocked down, and the wound healing assay showed that the migration of CIA FLS was also suppressed. Dual luciferase detection was used to confirm the relationship among NR-133666, miR-133c and MAPK1. MAPK1 is the target gene of miR-133c, where NR-133666 acts as a sponge of miR-133c to reduce the inhibitory effect of miR-133c on MAPK1. Overexpression of NR-133666 and MAPK1 can promote the proliferation and migration of CIA FLS, and overexpression of miR-133c can reverse this phenomenon. Western blot indicated that it may be related to the ERK/MAPK signaling pathway. Collectively, we identified that lncRNA NR-133666 acted as a miR-133c sponge that can promote the proliferation and migration of CIA FLS through regulating the miR-133c/MAPK1 axis.
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): Transcriptome Assembly for the Development of Pest Control Strategies
Authors: Schvartzman Claudia , Fresia Pablo , Murchio Sara , Mujica María Valentina , Dalla-Rizza Marco
Published: April 2022

Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
The Evolution of Cytogenetic Traits in Cuscuta (Convolvulaceae), the Genus With the Most Diverse Chromosomes in Angiosperms
Authors: Ibiapino Amalia , García Miguel A. , Amorim Bruno , Baez Mariana , Costea Mihai , Stefanović Saša , Pedrosa-Harand Andrea
Published: April 2022

Karyotypes are characterized by traits such as chromosome number, which can change through whole-genome duplication and dysploidy. In the parasitic plant genus Cuscuta (Convolvulaceae), chromosome numbers vary more than 18-fold. In addition, species of this group show the highest diversity in terms of genome size among angiosperms, as well as a wide variation in the number and distribution of 5S and 35S ribosomal DNA (rDNA) sites. To understand its karyotypic evolution, ancestral character state reconstructions were performed for chromosome number, genome size, and position of 5S and 35S rDNA sites. Previous cytogenetic data were reviewed and complemented with original chromosome counts, genome size estimates, and rDNA distribution assessed via fluorescence in situ hybridization (FISH), for two, seven, and 10 species, respectively. Starting from an ancestral chromosome number of x = 15, duplications were inferred as the prevalent evolutionary process. However, in holocentric clade (subgenus Cuscuta), dysploidy was identified as the main evolutionary mechanism, typical of holocentric karyotypes. The ancestral genome size of Cuscuta was inferred as approximately 1C = 12 Gbp, with an average genome size of 1C = 2.8 Gbp. This indicates an expansion of the genome size relative to other Convolvulaceae, which may be linked to the parasitic lifestyle of Cuscuta. Finally, the position of rDNA sites varied mostly in species with multiple sites in the same karyotype. This feature may be related to the amplification of rDNA sites in association to other repeats present in the heterochromatin. The data suggest that different mechanisms acted in different subgenera, generating the exceptional diversity of karyotypes in Cuscuta.
Characterisation of the Introgression of Brassica villosa Genome Into Broccoli to Enhance Methionine-Derived Glucosinolates and Associated Health Benefits
Authors: Neequaye Mikhaela , Steuernagel Burkhard , Saha Shikha , Trick Martin , Troncoso-Rey Perla , van den Bosch Frans , Traka Maria H. , Østergaard Lars , Mithen Richard
Published: April 2022

Broccoli cultivars that have enhanced accumulation of methionine-derived glucosinolates have been developed through the introgression of a novel allele of the MYB28 transcription factor from the wild species Brassica villosa. Through a novel k-mer approach, we characterised the extent of the introgression of unique B. villosa genome sequences into high glucosinolate broccoli genotypes. RNAseq analyses indicated that the introgression of the B. villosa MYB28 C2 allele resulted in the enhanced expression of the MYB28 transcription factor, and modified expression of genes associated with sulphate absorption and reduction, and methionine and glucosinolate biosynthesis when compared to standard broccoli. A adenine-thymine (AT) short tandem repeat (STR) was identified within the 5′ untranslated region (UTR) B. villosa MYB28 allele that was absent from two divergent cultivated forms of Brassica oleracea, which may underpin the enhanced expression of B. villosa MYB28.
Disaggregation of Ploidy, Gender, and Genotype Effects on Wood and Fiber Traits in a Diploid and Triploid Hybrid Poplar Family
Authors: Huang Xu-Yan , Shang Jing , Zhong Yu-Hang , Li Dai-Li , Song Lian-Jun , Wang Jun
Published: April 2022

Triploid breeding based on unilateral sexual polyploidization is an effective approach for genetic improvement of Populus, which can integrate heterosis and ploidy vigor in an elite variety. However, the phenotypic divergence of unselected allotriploids with the same cross-combination remains poorly understood, and the contributions of ploidy, gender, and genotype effects on phenotypic variation are still unclear. In this study, wood and fiber traits, including basic density (BD), lignin content (LC), fiber length (FL), fiber width (FW), and fiber length/width (FL/W), were measured based on a 10-year-old clonal trial, including full-sib diploid and triploid hybrids of (Populus pseudo-simonii × P. nigra ‘Zheyin3#’) × P. × beijingensis, and contributions of ploidy, gender, and genotype effects on the variation of these traits, were disaggregated to enhance our understanding of triploid breeding. We found a significant phenotypic variation for all measured traits among genotypes. All the wood and fiber traits studied here underwent strong clonal responses with high repeatabilities (0.55–0.76). The Pearson’s correlation analyses based on the best linear unbiased predictors (BLUPs) revealed that BD was significantly positively correlated with FL (r = 0.65, p = 0.030), suggesting that BD could be improved together with FL during triploid breeding. The FL of the triploids was significantly larger than that of the diploids (p < 0.001), suggesting that ploidy strongly affected the variation of FL traits. The difference between females and males was not significant for any measured trait, implying that gender might not be a major factor for variation in these traits. Further analyses of variance components showed that genotype dominantly contributed to the variation of BD, LC, and FW traits (with 54, 62, and 53% contributions, respectively) and ploidy contributed strongly to variation in FL and FL/W (77 and 50%, respectively). The genetic coefficient of variation (CVG) of triploids for each trait was low, suggesting that it is necessary to produce many triploids for selection or to use different Populus species as parents. Our findings provide new insights into the genetic effects of ploidy, gender, and genotype on wood and fiber traits within a full-sib poplar family, enhancing the understanding of the triploid breeding program of Populus.
Examining Motor Anticipation in Handwriting as an Indicator of Motor Dysfunction in Schizophrenia
Authors: Crespo Cobo Yasmina , Kandel Sonia , Soriano María Felipa , Iglesias-Parro Sergio
Published: April 2022

Dysfunction in motor skills can be linked to alterations in motor processing, such as the anticipation of forthcoming graphomotor sequences. We expected that the difficulties in motor processing in schizophrenia would be reflected in a decrease of motor anticipation. In handwriting, motor anticipation concerns the ability to write a letter while processing information on how to produce the following letters. It is essential for fast and smooth handwriting, that is, for the automation of graphomotor gestures. In this study, we examined motor anticipation by comparing the kinematic characteristics of the first l in the bigrams ll and ln written on a digitiser. Previous studies indicated that the downstroke duration of the first l is modulated by the anticipation of the local constraints of the following letter. Twenty-four adult individuals with diagnosis of schizophrenia and 24 healthy adults participated in the study. The classic measures of duration (sec), trajectory (cm), and dysfluency (velocity peaks) were used for the kinematic analysis of the upstroke (US) and downstroke (DS). In the control group, the duration of the downstroke of the l was longer in ln than ll (US: ln = ll; DS: ln > ll) whereas no differences were found for the group with schizophrenia. Likewise, the control group showed a longer DS trajectory for the l of ln than ll in downstrokes, while the group of patients failed to show this effect. These results suggest that the motor alterations in patients with schizophrenia could also affect their ability for motor anticipation.
Exposure to Olfactory Alcohol Cues During Non-rapid Eye Movement Sleep Did Not Decrease Craving in Patients With Alcohol Dependence
Authors: Zhu Ran , Ni Zhaojun , Tao Ran , Cheng Jun , Pang Liangjun , Zhang Shun , Zhang Yang , Xue Yanxue , Ma Yundong , Sun Wei , Lu Lin , Deng Jiahui , Sun Hongqiang
Published: April 2022

Background and Objectives

Cue exposure therapy (CET) has been used to reduce alcohol use, but the effect of CET during sleep on alcohol dependence (AD) is unclear. The present study examined the effect of repeated exposure to an olfactory stimulus during non-rapid eye movement (NREM) sleep on cue reactivity and craving in patients with AD.

Methods

Thirty-five patients with AD were enrolled according to the Diagnostic and Statistical Manual of Mental Disorders-IV (DSM-IV). All the subjects were randomly assigned to the experimental or control group. The experimental group was exposed to alcohol odor for 10 min during NREM sleep. The other group (controls) was exposed to water [control stimulus (CtrS)] for 10 min during NREM sleep. Demographic, alcohol-related, and clinical characteristics were collected at baseline. A cue-reactivity test was conducted before and after exposure to evaluate the effect of memory manipulation on acute response to an alcohol stimulus.

Results

There were no significant time × group interactions according to the visual analog scale (VAS) score of craving intensity, skin conductance response (SCR), systolic blood pressure (SBP), and diastolic blood pressure (DBP; all p > 0.05). Two-way ANOVA showed significant main effects of time on SCR [F(1,33) = 4.453, p = 0.043], SBP [F(1,33) = 14.532, p = 0.001], DBP [F(1,33) = 8.327, p = 0.007], Craving-VAS [F(1,33) = 1.997, p = 0.167] in two groups.

Conclusion

Exposure to olfactory alcohol cues during NREM sleep had no significant effect on alcohol craving in subjects with AD during hospitalization.
Highlighting “Risky Remands” Through Prisoner Death Investigations: People With Very Severe Mental Illness Transitioning From Police and Court Custody Into Prison on Remand
Authors: Tomczak Philippa
Published: April 2022

Prison suicide/self-inflicted death is an international public health crisis, harming stakeholders including bereaved families, prisoners, prison staff and death investigators. England and Wales' record prison suicide numbers in 2016 cost at least £400 million. Death rates are an indicator of prison safety, and unsafe prisons mean unsafe societies. I present four case studies of people with very severe mental illness who were remanded to prison from police and/or court custody and went on to take their own lives in prison. I use publicly available data from Ombudsman and Coronial death investigations in England and Wales, highlighting that these accessible sources could be more widely mobilized to reduce the substantial harms and costs of prisoner deaths. Case studies include three men (Lewis Francis, Jason Basalat and Dean Saunders) and one woman (Sarah Reed) who took their own lives between January 2016 and April 2017. All four people were clearly very mentally unwell at the time of their alleged offense and remand to prison. I develop the concept of “risky remands” to highlight that people with very severe mental illness being remanded to prison is a particularly problematic practice. I highlight the implications of people with very severe mental illness transitioning into prison in the first place, arguing that being remanded to prison is not an acceptable or safe pathway into healthcare. I illustrate that police custody suites and courts may lack awareness of mechanisms and/ or the practical ability to transfer ill detainees charged with a serious crime to mental health facilities for assessment and/ or treatment. My analysis amplifies and extends recent Criminal Justice Joint Inspection findings that it is unacceptable to use prisons as a “place of safety,” and that the Department of Health and Social Care, NHS England and the Welsh Government must increase the supply of medium and high secure beds. Moreover, Ombudsman investigations did not engage with the remand transition, effectively legitimizing this risky practice for very ill people. As such, my analysis also counters the apparent “problem of implementation” in prison oversight, instead questioning what reviewers recommend, based on which evidence.
Proactive Innovation in a Prolonged Conflict Setting: Facing COVID-19 in a Specialized Cancer Hospital in Palestine
Authors: Sabateen Ali , Khalil Merette , Abu El Hawa Munia , Peeperkorn Richard , Mataria Awad , Ravaghi Hamid
Published: April 2022

The prolonged ongoing conflict in Palestine exacerbated socioeconomic conditions and weakened the health system, complicating the management of COVID-19 pandemic, especially for cancer patients who are doubly-at risk. Augusta Victoria Hospital (AVH) is Palestine's only specialized cancer hospital, receiving patients from the Gaza Strip and the West Bank for oncology, nephrology, hematology, and radiotherapy. AVH's preparedness measures enabled its agile response. These proactive and innovative preparedness measures included: implementing a facility-level preparedness and response plan; utilizing multidisciplinary team-based and evidence-informed approaches to decision making; prioritizing health workers' safety and education; establishing in-house PCR testing to scale up timely screenings; and accommodating health workers, patients, and their relatives at hospital hotels, to maintain daily, continuous and critical health care for cancer patients and limit the spread of infection. At the facility-level, the biggest challenge faced by AVH was continuing essential and daily care for immunocompromised patients while protecting them from potential infection from relatives, hospital staff and other suspected patients. At the national level, the lack of preparedness, inequalities in vaccine distribution, political instability, violence, delays in obtaining medical exit permits to reach Jerusalem, weakened AVH's response. AVH's flexible financing, hospital accreditation, and strong leadership and coordination enabled its agility and resilience. Despite compiling challenges, the hospital's proactive and innovative interventions minimized the risk of infection among two high-risk groups: the immunocompromised patients and their health workers, providing invaluable lessons for health facilities in other fragile-and-conflict-affected settings.
Scaling and Disagreements: Bias, Noise, and Ambiguity
Authors: Uma Alexandra , Almanea Dina , Poesio Massimo
Published: April 2022

Crowdsourced data are often rife with disagreement, either because of genuine item ambiguity, overlapping labels, subjectivity, or annotator error. Hence, a variety of methods have been developed for learning from data containing disagreement. One of the observations emerging from this work is that different methods appear to work best depending on characteristics of the dataset such as the level of noise. In this paper, we investigate the use of an approach developed to estimate noise, temperature scaling, in learning from data containing disagreements. We find that temperature scaling works with data in which the disagreements are the result of label overlap, but not with data in which the disagreements are due to annotator bias, as in, e.g., subjective tasks such as labeling an item as offensive or not. We also find that disagreements due to ambiguity do not fit perfectly either category.
Comparing Multi-Arm Robotics for In-Space Assembly
Authors: McBryan Katherine
Published: April 2022

Robotic In-space assembly (ISA) is the next step to building larger and more permanent structures in orbit. Determining the best robot for ISA is difficult as it will not only depend on the structure being assembled but on how it is assembled. This analysis shows how changing some key design parameters can influence different robotic systems for ISA. This study focuses on the construction of a 20 m linear truss structure but also expands to a 10 and 50 m truss. Two categories of robots are included in this study: a stationary robot and a mobile robot which crawls along the structure. Both the stationary and crawling robotic systems utilize two planar dexterous manipulators to assemble individual truss pieces into a linear truss. In the case of the stationary robotic system a single long positioning leg is used to move the two dexterous arms into position. The crawling robotic system uses two planar manipulators to crawl along the truss. A systems level analysis is presented which details how the forces from the robotic systems drive the mass of the truss and also how the size of the truss segments drive the requirements of the robotic system. This analysis shows how changing some key design parameters can influence each of the different robotic systems and the truss design itself. The estimated masses of the robotic systems and the truss and the assembly time are presented. There are trade-offs to every robot design and understanding those trade-offs is essential to building a system that is not only efficient but also cost-effective.
Spies in the Deep: Ocean Landers Explore the Deep Sea
Authors: Yang Haleigh T. , Hardy Kevin , Wegner Nicholas C. , Nicoll Ashley , Levin Lisa A. , Gallo Natalya D.
Published: April 2022

Below the surface layers of the ocean, there are ecosystems full of undiscovered life. Scientists love to ask questions like, “Who is there?” and “What are they doing?” An important question scientists are beginning to ask is, “How will these living things react to warmer waters, loss of oxygen, or pollution?” To answer these questions, scientists build equipment to observe life in the deep sea. We built an ocean lander named BEEBE, with a camera, sensors, and waterproof casing. BEEBE helped us study deep-sea ecosystems near the coast of California and learn about the animals that live there. We can use what we learned to recognize vulnerable communities and the threats some ocean animals face. An ocean lander like BEEBE can be a great tool to learn more about coastal, deep-sea ecosystems around the world!
Are All Sponges Spongy?
Authors: Schuster Astrid , Strehlow Brian W. , Perrigo Allison
Published: April 2022

Sponges are animals that live in oceans, lakes, and rivers. There are close to 10,000 sponge species described by scientists, but far more species await discovery! However, not all sponges look and feel like their kitchen-sink cousins. Over time, sponges have evolved into many sizes and shapes, giving us the huge diversity of sponge species on Earth today. For example, many sponges are rock-hard! These rock sponges use minerals like silica (glass) to build their skeletons—instead of bone like ours. Other sponges have evolved unique ways to eat. Most sponges filter water to get their food, but certain sponges are carnivorous, meaning that they eat meat, including tiny shrimp-like creatures. Sponge species with different body and skeleton shapes and sizes can thrive in very different and unique environments.
Case Report: Congenital Intracranial Kaposiform Hemangioendothelioma Treated With Surgical Resection
Authors: Cai Yingjie , Li Jiayi , Yang Wei , Zhang Nan , Sun Hailang , Zhang Weiping , Ge Ming
Published: April 2022

Background

Kaposiform hemangioendothelioma (KHE) is a locally aggressive but non-metastatic vascular neoplasm. Most studies have been restricted to small case series of limited generalizability. Intracranial KHE is extremely rare with only three cases reported in the literature. Here, we report a case of congenital intracranial KHE who underwent surgical resection, and no lesion recurrence was seen during the follow-up period of 13 months.

Case Description

A 2-month-old boy initially presented with a left temporal mass following birth. Antenatal ultrasound at 36 weeks of gestation demonstrated a hyperechoic signal present in the left frontal lobe, with clear borders and irregular morphology. There were neither cutaneous abnormalities nor other neurologic examination abnormalities. No laboratory abnormality was identified. Computed tomography (CT) scans suggested that a massive hematoma was noted under the left frontal skull plate, with a little subdural hemorrhage in the adjacent temporal area. The adjacent meninges enhanced and thickened on contrasted T1 magnetic resonance (MR). After the multidisciplinary diagnostic assessment, the surgery was performed by the left frontotemporal craniotomy approach. The operation was extremely difficult. We completely removed the tumor, and the involved dura and brain tissue were resected with the lesion in a piecemeal fashion. On postoperative-day (POD) 3 and POD 14, the head CT re-examination revealed that cerebral perfusion improved gradually. The MRI of 6- and 12-month after operation showed no local recurrence or metastasis.

Conclusions

Intracranial KHE is difficult to diagnose early and the prognosis has been uniformly poor. We supposed that meticulous intraoperative hemostasis is the key for a successful operation, and the radical resection of the tumor and involved structures are essential to reduce recurrence.
Astaxanthin Supplementation Improves the Subsequent Developmental Competence of Vitrified Porcine Zygotes
Authors: Xiang Decai , Jia Baoyu , Zhang Bin , Liang Jiachong , Hong Qionghua , Wei Hongjiang , Wu Guoquan
Published: April 2022

Cryopreservation of embryos has been confirmed to cause oxidative stress as a factor responsible for impaired developmental competence. Currently, astaxanthin (Ax) raises considerable interest as a strong exogenous antioxidant and for its potential in reproductive biology. The present study aimed to investigate the beneficial effects of Ax supplementation during in vitro culture of vitrified porcine zygotes and the possible underlying mechanisms. First, the parthenogenetic zygotes were submitted to vitrification and then cultured in the medium added with various concentrations of Ax (0, 0.5, 1.5, and 2.5 μM). Supplementation of 1.5 μM Ax achieved the highest blastocyst yield and was considered as the optimal concentration. This concentration also improved the blastocyst formation rate of vitrified cloned zygotes. Moreover, the vitrified parthenogenetic zygotes cultured with Ax exhibited significantly increased mRNA expression of CDX2, SOD2, and GPX4 in their blastocysts. We further analyzed oxidative stress, mitochondrial and lysosomal function in the 4-cell embryos and blastocysts derived from parthenogenetic zygotes. For the 4-cell embryos, vitrification disturbed the levels of reactive oxygen species (ROS) and glutathione (GSH), and the activities of mitochondria, lysosome and cathepsin B, and Ax supplementation could fully or partially rescue these values. The blastocysts obtained from vitrified zygotes showed significantly reduced ATP content and elevated cathepsin B activity, which also was recovered by Ax supplementation. There were no significant differences in other parameters mentioned above for the resultant blastocysts. Furthermore, the addition of Ax significantly enhanced mitochondrial activity and reduced lysosomal activity in resultant blastocysts. In conclusion, these findings revealed that Ax supplementation during the culture period improved subsequent embryonic development and quality of porcine zygotes after vitrification and might be used to ameliorate the recovery culture condition for vitrified embryos.
Effect of Sulfur-Enriched Vermicompost on the Growth of Brassica chinensis L. and the Spodoptera litura Fabricius Larvae Feeding
Authors: Fong Chang-Jun , Chuang Yi-Yuan , Lai Hung-Yu
Published: April 2022

(1) Background: Vermicompost is enriched with plant essential nutrients and has been shown to suppress the incidence of pests; however, its potential is affected by its food sources. (2) Methods: Earthworms were fed cabbage or pig manure to produce two vermicomposts enriched in sulfur and nutrients, respectively. A pot experiment and a feeding experiment were then conducted to determine whether the application of the vermicomposts and sulfur could increase soil fertility, promote the growth of Brassica chinensis L., and inhibit the growth of Spodoptera litura Fabricius larvae. (3) Results: The characteristics of the vermicomposts were mainly affected by the food sources, and vermicomposted cabbage was found to have a higher sulfur content than vermicomposted pig manure. The application of the vermicomposts enhanced the concentrations of organic matter and available phosphorus, as well as the exchange concentrations of potassium, cadmium, and magnesium in the soil. Moreover, the growth of and the accumulated phosphorus and sulfur in the B. chinensis L. samples significantly increased when the plants were grown in soils treated with the two vermicomposts. Hence, the addition of vermicomposted cabbage and sulfur fertilizers can decrease the relative growth rate, total consumption, efficiency of conversion of ingested food, and relative consumption rate of S. litura larvae, possibly due to the increase in leaf sulfur concentration.
Genomic Association Mapping of Apparent Amylose and Protein Concentration in Milled Rice
Authors: Alpuerto Jasper Benedict B. , Samonte Stanley Omar P. B. , Sanchez Darlene L. , Croaker Peyton A. , Wang Ya-Jane , Wilson Lloyd T. , Christensen Eric F. , Tabien Rodante E. , Yan Zongbu , Thomson Michael J.
Published: April 2022

Apparent amylose and protein concentrations (AAC and PC) affect the milling, cooking, and eating quality of rice. This study was conducted to assess the phenotypic and genotypic variation and to identify genomic regions and putative candidate genes associated with AAC and PC in milled rice grain. Two hundred and seventeen and 207 diverse rice accessions were grown at the Texas A&M AgriLife Research Center in 2018 and 2019, respectively. Milled rice samples were analyzed for AAC and PC using the iodine colorimetry and Dumas method, respectively. Genome-wide association studies (GWAS) for AAC and PC were conducted using 872,556 single nucleotide polymorphism (SNP) markers following the mixed linear model. Significant variation among the accessions was found for both variables each year. Associations between 32 SNPs with PC and seven SNPs with AAC were detected. Gene models linked to these SNPs have a wide range of biological functions, including protein and carbohydrate metabolism, DNA methylation, and response to abiotic and biotic stresses. Seven of the identified SNPs colocalized with previously reported quantitative trait loci (QTL) for protein concentration. Fine-mapping of significant genomic regions and gene validation are necessary for this information to aid in marker-assisted breeding for improved grain quality.
Describing Lettuce Growth Using Morphological Features Combined with Nonlinear Models
Authors: Li Qinglin , Gao Hongyan , Zhang Xiaodong , Ni Jiheng , Mao Hanping
Published: April 2022

The aim of this study was to describe the sigmoidal growth behaviour of a lettuce canopy using three nonlinear models. Gompertz, Logistic and grey Verhulst growth models were established for the top projected canopy area (TPCA), top projected canopy perimeter (TPCP) and plant height (PH), which were measured by two machine vision views and 3D point clouds data. Satisfactory growth curve fitting was obtained using two evaluation criteria: the coefficient of determination (R2) and the mean absolute percentage error (MAPE). The grey Verhulst models produced a better fit for the growth of TPCA and TPCP, with higher R2 (RTPCA2=0.9097, RTPCP2=0.8536) and lower MAPE (MAPETPCA=0.0284, MAPETPCP=0.0794) values, whereas the Logistic model produced a better fit for changes in PH (RPH2=0.8991, MAPEPH=0.0344). The maximum growth rate point and the beginning and end points of the rapid growth stage were determined by calculating the second and third derivatives of the models, permitting a more detailed description of their sigmoidal behaviour. The initial growth stage was 1–5.5 days, and the rapid growth stage lasted from 5.6 to 26.2 days. After 26.3 days, lettuce entered the senescent stage. These inflections and critical points can be used to gain a better understanding of the growth behaviour of lettuce, thereby helping researchers or agricultural extension agents to promote growth, determine the optimal harvest period and plan commercial production.
Soil Water Dynamics, Effective Rooting Zone, and Evapotranspiration of Sprinkler Irrigated Potato in a Sandy Loam Soil
Authors: Djaman Koffi , Koudahe Komlan , Saibou Aminou , Darapuneni Murali , Higgins Charles , Irmak Suat
Published: April 2022

Potato (Solanum tuberosum L.) is a very sensitive crop to water stress and timely irrigation water management improves tuber yield and quality. The objectives of this study were to (1) investigate soil water dynamics under potato crops across their root zone and (2) estimate potato crop evapotranspiration (ETa) under sprinkler irrigation on the sandy loam soil. The field experiment was conducted during the 2018 and 2019 growing seasons at the Navajo Farms within the Navajo Agricultural Products Industry, Farmington, NM. Two irrigation scheduling methods were evaluated as FAO-56 approach evapotranspiration-based scheduling and soil moisture sensing irrigation scheduling. Sentek capacitance soil moisture probe was used across four commercial potato fields in each year after calibration to the soil texture just after installation. Crop Evapotranspiration values estimated by the water balance method and the two-step approach were compared to the satellite-based models used in OpenET. The results showed that the potato’s effective rooting zone is the upper 40 cm soil layer. Potato plants extracted more than 50% of total water from the upper 15 cm of the soil profile and about 85% from the upper 40 cm of the soil profile. Little water amount was extracted from the 40–60 cm soil water. Potato crop seasonal evapotranspiration averaged 580 to 645 mm in 2018 and 2019, respectively. The Two-step approach ETa values of 795.5 and 832.7 mm in 2018 and 2019, respectively, were higher than the soil water balance estimated ETa. The satellite modeled ETa varied with field and years and ranged from 437 to 759 mm and averaged 570.4 mm for the 2016–2020 period. Soil moisture probe-based irrigation scheduling improved irrigation water management and the irrigation water use of potatoes in the semiarid climate.
Conservation Agriculture and Agroecological Weed Management
Authors: Cordeau Stéphane
Published: April 2022

Conservation agriculture (CA) relies on three fundamental pillars [...]
Impact of Feeding Fermented Palm Kernel Cake and High Dietary Fat on Nutrient Digestibility, Enzyme Activity, Intestinal Morphology and Intestinal Nutrient Transporters mRNA Expression in Broiler Chickens under Hot and Humid Conditions
Authors: Hakim Ali , Zulkifli Idrus , Farjam Abdoreza , Awad Elmutaz , Ramiah Suriyah
Published: April 2022

The study aimed at determining the ileal nutrient digestibility, digestive enzyme activity, intestinal morphology, and nutrient transporters mRNA expressions in broiler chickens fed with fermented PKC (LPKC) based diets with different levels of fat supplementation under hot and humid conditions. From day 22 to 35, broiler chickens were randomly fed with either (1) 20% LPKC-based diet with 5% palm oil, (2) 20% LPKC based diet with 9.5% palm oil, (3) 20% PKC-based diet with 5% palm oil or (4) 20% PKC-based diet with 9.5% palm oil. Feeding LPKC and PKC diets at the finisher phase have not affected the nutrient’s digestibility, but a higher level of oil supplementation does. This was seconded by changes in the digestive enzyme activity, villus height, and mRNA expression of nutrient transporters in the higher level of oil-supplemented diets fed chickens. In conclusion, the inclusion of oil at 9.5% in a 20% LPKC/PKC-based diet is necessary to ensure better nutrient digestibility in chickens via improved digestive function, especially in hot and humid tropical regions.
Changes to Gut Microbiota Following Systemic Antibiotic Administration in Infants
Authors: Kwon Yoowon , Cho Young-Sun , Lee Yoo-Mi , Kim Seok-jin , Bae Jaewoong , Jeong Su-Jin
Published: April 2022

Long-term antibiotic use can have consequences on systemic diseases, such as obesity, allergy, and depression, implicating the causal role of gut microbiome imbalance. However, the evaluation of the effect of antibiotics in early infancy on alterations to the gut microbiome remains poorly understood. This study aimed to evaluate the gut microbiome state in infancy following systemic antibiotic treatment. Twenty infants under 3 months of age who had received antibiotics for at least 3 days were enrolled, and their fecal samples were collected 4 weeks after antibiotic administration finished. Thirty-four age-matched healthy controls without prior exposure to antibiotics were also assessed. The relative bacterial abundance in feces was obtained via sequencing of 16 S rRNA genes, and alpha and beta diversities were evaluated. At the genus level, the relative abundance of Escherichia/Shigella and Bifidobacterium increased (p = 0.03 and p = 0.017, respectively) but that of Bacteroides decreased (p = 0.02) in the antibiotic treatment group. The microbiome of the antibiotic treatment group exhibited an alpha diversity lower than that of the control group. Thus, systemic antibiotic administration in early infancy affects the gut microbiome composition even after a month has passed; long-term studies are needed to further evaluate this.
Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2
Authors: Jin Jun-Xue , Sun Jing-Tao , Jiang Chao-Qian , Cui Hong-Di , Bian Ya , Lee Sanghoon , Zhang Lianjin , Lee Byeong Chun , Liu Zhong-Hua
Published: April 2022

Previous studies suggest that the inclusion of melatonin (MTn) in in vitro maturation protocols improves the developmental competence of oocytes by scavenging reactive oxygen species (ROS). However, the molecular mechanisms integrating melatonin receptor (MT)-mediated lipid metabolism and redox signaling during in vitro cumulus–oocyte complex (COC) development still remain unclear. Here, we aimed to elucidate the potential role of MTn receptors in lipid metabolic adjustments during in vitro porcine COC development. We observed that MTn-mediated Gsα–cAMP/PKA signaling facilitated lipolysis primarily through the MT2 receptor and subsequently increased fatty acid (FA) release by hydrolyzing intracellular triglycerides (TGs) in cumulus cells. Furthermore, CD36 was a critical FA transporter that transported available FAs from cumulus cells to oocytes and promoted de novo TG synthesis in the latter. In addition, MTn regulated lipogenesis and intracellular lipolysis to maintain lipid homeostasis and limit ROS production, thereby supporting oocyte cytoplasmic maturation and the subsequent embryo development. Taken together, these findings provide insight into the possible mechanism integrating MT2-mediated lipid homeostasis and redox signaling, which limits ROS production during in vitro COC development. Therefore, understanding the dynamics of the interactions between lipid homeostasis and redox signaling driven by MT2 is necessary in order to predict drug targets and the effects of therapeutics used to improve female reproductive health.
Agaricus Mushroom-Enriched Diets Modulate the Microbiota-Gut-Brain Axis and Reduce Brain Oxidative Stress in Mice
Authors: García-Sanmartín Josune , Bobadilla Miriam , Mirpuri Eduardo , Grifoll Vanessa , Pérez-Clavijo Margarita , Martínez Alfredo
Published: April 2022

Neurodegenerative diseases pose a major problem for developed countries, and stress has been identified as one of the main risk factors in the development of these disorders. Here, we have examined the protective properties against brain oxidative stress of two diets supplemented with 5% (w/w) of Agaricus bisporus (white button mushroom) or Agaricus bisporus brunnescens (Portobello mushroom) in mice. These diets did not modify the weight gain of the animals when compared to those fed with a regular diet, even after feeding on them for 15 weeks. The long-term modification of the microbiota after 12 weeks on the diets was investigated. At the phylum level, there was a large increase of Verrucomicrobia and a reduction of Cyanobacteria associated with the mushroom diets. No changes were observed in the Firmicutes/Bacteroidetes ratio, whose stability is a marker for a healthy diet. At the family level, three groups presented significant variations. These included Akkermansiaceae and Tannerellaceae, which significantly increased with both diets; and Prevotellaceae, which significantly decreased with both diets. These bacteria participate in the generation of microbiota-derived short-chain fatty acids (SCFAs) and provide a link between the microbiota and the brain. Mice subjected to restraint stress showed an upregulation of Il-6, Nox-2, and Hmox-1 expression; a reduction in the enzymatic activities of catalase and superoxide dismutase; and an increase in lipid peroxidation in their brains. All these parameters were significantly prevented by feeding for 3 weeks on the Agaricus-supplemented diets. In summary, the supplementation of a healthy diet with Agaricus mushrooms may significantly contribute to prevent neurodegenerative diseases in the general population.