bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes, Mortality Causes, and Pathological Findings in European Hedgehogs (Erinaceus europaeus, Linnaeus 1758): A Seventeen Year Retrospective Analysis in the North of Portugal
Authors: Garcês Andreia , Soeiro Vanessa , Lóio Sara , Sargo Roberto , Sousa Luís , Silva Filipe , Pires Isabel
Published: July 2020

This study aimed to analyze the admission causes, outcomes, primary causes of death, and main lesions observed in the post mortem examinations of Western European hedgehogs, Erinaceus europaeus (Linnaeus, 1758), in the north of Portugal. The data were obtained by consulting the records from the two main wildlife rehabilitation centers located in the north of Portugal (Wildlife Rehabilitation Centre of Parque Biologico de Gaia and the Wildlife Rehabilitation Centre of the University of Trás-os-Montes and Alto Douro). Over 17 years (2002–2019) a total of 740 animals were admitted. Most of the animals were juveniles, with the highest number of admissions occurring during summer (36.8%) and spring (33.2%). The main reasons for admission were debilitation (30.7%) and random finds (28.4%). Of the total number of individuals admitted to these centers, 66.6% were successfully released back into the wild. The most relevant causes of death were trauma of unknown origin (32.7%), nontrauma causes of unknown origin (26.6%), and nutritional disorders (20.2%). The main lesions observed were related to trauma, including skeletal and skin lesions (fractures, hemorrhages, wounds) and organ damage, particularly to the lungs and liver. The hedgehog is a highly resilient and adaptable animal. The urban environment has many benefits for hedgehogs, yet the presence of humans can be harmful. In the future, the public needs to become even more involved in the activities of the wildlife centres, which will make a positive difference for these populations.
The pathogen Mycoplasma dispar Shows High Minimum Inhibitory Concentrations for Antimicrobials Commonly Used for Bovine Respiratory Disease
Authors: Bottinelli Marco , Merenda Marianna , Gastaldelli Michele , Picchi Micaela , Stefani Elisabetta , Nicholas Robin A. J. , Catania Salvatore
Published: July 2020

Mycoplasma dispar is an overlooked pathogen often involved in bovine respiratory disease (BRD), which affects cattle around the world. BRD results in lost production and high treatment and prevention costs. Additionally, chronic therapies with multiple antimicrobials may lead to antimicrobial resistance. Data on antimicrobial susceptibility to M. dispar is limited so minimum inhibitory concentrations (MIC) of a range of antimicrobials routinely used in BRD were evaluated using a broth microdilution technique for 41 M. dispar isolates collected in Italy between 2011–2019. While all isolates had low MIC values for florfenicol (<1 μg/mL), many showed high MIC values for erythromycin (MIC90 ≥8 μg/mL). Tilmicosin MIC values were higher (MIC50 = 32 μg/mL) than those for tylosin (MIC50 = 0.25 μg/mL). Seven isolates had high MIC values for lincomycin, tilmicosin and tylosin (≥32 μg/mL). More, alarmingly, results showed more than half the strains had high MICs for enrofloxacin, a member of the fluoroquinolone class considered critically important in human health. A time-dependent progressive drift of enrofloxacin MICs towards high-concentration values was observed, indicative of an on-going selection process among the isolates.
Design and Construction of a Low-Cost Test Bench for Testing Agricultural Spray Nozzles
Authors: Longo Domenico , Manetto Giuseppe , Papa Rita , Cerruto Emanuele
Published: July 2020

Droplet size distribution is probably the most important feature of a spray as it affects all aspects of a phytosanitary treatment, i.e., biological, environmental, and safety aspects. This study describes a low-cost laboratory test bench able to analyze agricultural spray nozzles under realistic conditions. The design of the equipment was mainly based on the ISO 5682-1 standard. It has a couple of 3 m long rails, along which the nozzle under test moves while spraying, controlled by a closed-loop position and speed controller. The drops were captured with three Petri dishes containing silicone oil, photographed by means of a digital single-lens reflex (DSLR) camera, and then analyzed with the ImageJ software in order to measure the usual spray parameters: the volumetric diameters, the Sauter mean diameter, and the number mean diameter. Spray trials and tuning of the system parameters were managed by means of a purposely designed user interface running on a Windows 10 PC. Some tests were carried out by using an Albuz ATR80 orange hollow cone nozzle at the working pressures of 0.3, 0.5, 1.0, and 1.5 MPa. The results about spray quality agree with the factory information, and the whole system, even if some aspects still need improvements, has proven reliable.
An Electromagnetic–Thermal Coupling Numerical Study of the Synchronous Generator with Second-Generation High-Temperature Superconducting Armatures
Authors: Huang Xiangyu , Huang Zhen , Xu Xiaoyong , Li Wan , Jin Zhijian
Published: July 2020

Generators with high-temperature superconducting armatures have an advantage in the fact that they can carry high currents. However, the AC loss of high-temperature superconducting (HTS) armatures is difficult to calculate precisely because HTS coils exist in a complex and time-varying electromagnetic environment. In addition, when the HTS coil is carrying a short circuit fault overcurrent, an electromagnetic–thermal simulation study of this process is required to ensure that the HTS coil is not damaged. In this paper, first, a fully coupled T-A formulation model is used to calculate the AC loss of HTS armatures. Then, the current and temperature distributions are simulated, considering the intrinsic characteristic of superconducting coated conductors, when the generator suffers the worst short circuit fault accidently. It is found that the turn with the lowest critical current quenches after 0.01 s, but the temperature rise cannot damage the coil if the circuit breaker can clear the fault quickly. The effects of the copper stabilizer thickness on the thermal stability of the HTS coil during the worst short circuit fault are also investigated. A thicker copper stabilizer improves the thermal stability of the HTS coil in the event of a short circuit fault, but the use of a simulation model is needed to make trade-offs between the engineering current density and the thermal stability of the HTS tapes. The work in this paper is necessary and can provide an important reference for manufacturing superconducting generators.
Exploiting Nanoparticles to Improve the Properties of Bitumens and Asphalts: At What Extent Is It Really Worth It?
Authors: Calandra Pietro , Loise Valeria , Porto Michele , Oliviero Rossi Cesare , Lombardo Domenico , Caputo Paolino
Published: July 2020

Asphalt concretes are materials used worldwide. It is well-known that in such materials the minor component, the bitumen, plays the most important role since it binds the high fraction (>95%) of inorganic macrometer-sized particles ensuring a coherent material fit for uses in road pavement. Additives can be used to increase the overall rheological properties, with high benefits in terms of resistance to mechanical stress and to ageing. Among these, nanoparticles have recently been considered as very effective additives in increasing the overall performance, increasing the viscosity, the rutting parameter and the recovery from deformation. However, they are expensive, so a delicate equilibrium between costs and benefits must be found for large-scale uses. In this framework, we furnish our critical analysis of the state-of-the art technologies used for improving the bitumen performances by means of nanoparticles with an eye to eventual added-values (like anti-oxidant effect, antistripping properties, or UV radiation screening which avoids radiation-induced ageing…). We will critically consider the costs involved in their use and we will give our opinion about vanguard techniques which can be fit for the analysis of nanoparticles-containing bitumens and asphalts. Interesting perspectives will be also given for future research and applications.
Effect of the Leavening Agent on the Compositional and Sensorial Characteristics of Bread Fortified with Flaxseed Cake
Authors: Taglieri Isabella , Sanmartin Chiara , Venturi Francesca , Macaluso Monica , Zinnai Angela , Tavarini Silvia , Serra Andrea , Conte Giuseppe , Flamini Guido , Angelini Luciana G.
Published: July 2020

Health and well-being improvement is currently driving innovation in bread, using a wide variety of value-added compounds as extra ingredients, including food industry by-products in a circular economy concept. In this context, this research aimed at evaluating the effect of the fortification of bread with different percentages of flaxseed cake, comparing two leavening agents: sourdough and baker’s yeast. Sensorial, physicochemical, and nutritional properties, including pH, the main fermentative metabolites, fatty acids, total phenols, antioxidant capacity, and volatile organic compounds were determined for fortified bread. The results showed a significant improvement of nutraceutical profile of the bread fortified with flaxseed cake in a dose-dependent manner. Regardless of the leavening agent, the fortification determined a decrease of n-6:n-3 ratio, reaching the recommended value (<3) already at the 7.5% level. Furthermore, under the same fortification level, sourdough breads showed a higher level of total phenols and antiradical activity than baker’s yeast breads. Sensory profiles were instead deeply influenced by both the fortification percentage and the leavening agents. In conclusion, considering both nutritional and sensory results, the best formulation as a function of leavening agent utilized was defined as 5% and 7.5% when sourdough and baker’s yeast were used, respectively.
A Challenge: Support of Standing Balance in Assistive Robotic Devices
Authors: Lippi Vittorio , Mergner Thomas
Published: July 2020

Neurological patients using a powered lower-body exoskeleton for rehabilitation of standing and walking skills in an upright body pose face the safety challenge of postural instability and fall. Current research, therefore, develops exoskeletons with self-balancing functions. This study suggests basing the exoskeleton’s stabilization of standing posture on a human-derived postural control mechanism. A corresponding control system has previously been successfully tested with specific balancing tasks in humanoid robots. Here, we provide a short introduction into the control method and, using a lightweight robot, present as a test of the balancing an experimental shift in the body weight distribution (as if, e.g., a human exoskeleton user was raising an arm or leaning the upper body or lifting an external weight). An overview of other specific balancing tests previously already investigated in humans and humanoids is also briefly mentioned. Overall, the tests will allow the quantification of the capabilities of self-balancing exoskeletons developed for patients with partial paralysis of lower body sensorimotor functions.
CFD Simulation of a Temperature Control System for Galvanizing Line of Metal Band Based on Jet Cooling Heat Transfer
Authors: Carozzo Giovanni , Cravero Carlo , Marini Martino , Mazza Matteo
Published: July 2020

The work focuses on the development of a thermo-fluid dynamic simulation model of a section of close cooling, called a jet cooler, inserted in the galvanizing line of metal band production. Two models of increasing accuracy have been tested and calibrated by experimental data. Special attention to turbulence modeling and boundary conditions has been given. A literature survey was focused on the jet impingement process (the reference heat transfer mechanism for the system component) and on available correlations to predict the heat exchange coefficient. The numerical simulation of jet impingement has been applied to a module of an actual industrial cooler for steel band production. The operation of the jet cooler was simulated in real operating conditions to get a detailed insight into the jet impingement mechanism in order to optimize the heat transfer and reduce, as far as possible, the cooling fluid mass flow rate. The comparison of heat transfer correlations, used in industrial preliminary design, with detailed CFD results is discussed.
Spraying of Viscous Liquids: Influence of Fluid-Mixing Mechanism on the Performance of Internal-Mixing Twin-Fluid Atomizers
Authors: Mlkvik Marek , Jedelsky Jan , Karbstein Heike P. , Gaukel Volker
Published: July 2020

The thermal usage of liquid fuels implies their combustion, which is a process strongly influenced by the performance of the atomizer, which disrupts the fuel into drops of the required sizes. The spray quality of the twin-fluid atomizers with internal mixing (IM-TFA) is primarily influenced by the two-phase flow pattern inside the mixing chamber. We studied the performance of the four types of the IM-TFA nozzles by the optical diffraction system (Malvern Spraytec) to answer the question of how the mixing chamber design influences the spray quality at low atomizing gas consumption. We tested the effervescent atomizer in outside-in-liquid (OIL) and outside-in-gas (OIG) configurations, the Y-jet nozzle and new nozzle design, and the CFT atomizer when spraying model liquids with the viscosities comparable to the common fuels (μ=60and143 mPa· s). We found that the effervescent atomizer performance was strongly influenced by the configuration of the inlet ports. Although the OIL configuration provided the best spray quality (D32 = 72 μm), with the highest efficiency (0.16%), the OIG nozzle was characterized by unstable work and poor spray quality. Both the devices were sensitive to liquid viscosity. The Y-jet nozzle provided a stable performance over the liquid viscosity spectrum, but the spray quality and efficiency were lower than for the OIL nozzle. Our findings can be used to improve the performance of the common IM-TFA types or to design new atomizers. The results also provide an overview of the tested atomizers’ performances over the wide range of working conditions and, thus, help to define the application potential of the tested nozzle designs.
Edge-Event-Triggered Synchronization for Multi-Agent Systems with Nonlinear Controller Outputs
Authors: Liu Jie , Dai Ming-Zhe , Zhang Chengxi , Wu Jin
Published: July 2020

This paper addresses the synchronization problem of multi-agent systems with nonlinear controller outputs via event-triggered control, in which the combined edge state information is utilized, and all controller outputs are nonlinear to describe their inherent nonlinear characteristics and the effects of data transmission in digital communication networks. First, an edge-event-triggered policy is proposed to implement intermittent controller updates without Zeno behavior. Then, an edge-self-triggered solution is further investigated to achieve discontinuous monitoring of sensors. Compared with the previous event-triggered mechanisms, our policy design considers the controller output nonlinearities. Furthermore, the system’s inherent nonlinear characteristics and networked data transmission effects are combined in a unified framework. Numerical simulations demonstrate the effectiveness of our theoretical results.
Utilization of Partially Liquefied Bark for Production of Particleboards
Authors: Jiang Wen , Adamopoulos Stergios , Hosseinpourpia Reza , Žigon Jure , Petrič Marko , Šernek Milan , Medved Sergej
Published: July 2020

Bark as a sawmilling residue can be used for producing value-added chemicals and materials. This study investigated the use of partially liquefied bark (PLB) for producing particleboard with or without synthetic adhesives. Maritime pine (Pinus pinaster Ait.) bark was partially liquefied in the presence of ethylene glycol and sulfuric acid. Four types of particleboard panels were prepared with a PLB content of 4.7%, 9.1%, 20%, and 33.3%, respectively. Another five types of particleboard panels were manufactured by using similar amounts of PLB and 10 wt.% of melamine–urea–formaldehyde (MUF) adhesives. Characterization of bark and solid residues of PLB was performed by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and automated vapor sorption (AVS). Mechanical and physical properties of the particleboard were tested according to the European standards EN 310 for determining modulus of elasticity and bending strength, EN 317 for determining thickness swelling after immersion in water, and EN 319 for determining internal bond strength. The results showed that the increase in PLB content improved the mechanical strength for the non-MUF boards, and the MUF-bonded boards with up to 20% of PLB met the requirements for interior uses in dry conditions according to EN 312. The non-MUF boards containing 33.3% of PLB and the MUF-bonded boards showed comparable thickness swelling and water absorption levels compared to the reference board.
A Novel Method of Radar Emitter Identification Based on the Coherent Feature
Authors: Xue Jian , Tang Lan , Zhang Xinggan , Jin Lin
Published: July 2020

To deal with the problem of reliability degradation of radar emitter identification (REID) based on the traditional five parameters in a complex electromagnetic environment, a new feature extraction method based on the autocorrelation function of coherent signals, which makes full use of the coherent characteristic of modern radar emitters, is proposed in this paper. The main idea of this paper is utilizing the instantaneous autocorrelation function to obtain the correlation results of coherent and noncoherent signals. To this end, a new feature parameter, named the ratio of the secondary peak value to the main peak value (SMR), is defined to describe the difference of correlation results between coherent and noncoherent signals. Through simulation analysis, the feasibility of using SMR as the coherent feature for REID is verified. In order to evaluate the effectiveness of the coherent feature, an analytical hierarchy process (AHP) was introduced to compare the comprehensive performance of the coherent feature and the existing parameters, and then convolution neural network (CNN) and support vector machine (SVM) were selected as the classifier to check the recognition capability of the proposed feature. Simulation results show that the proposed feature can not only be used as a new feature for REID but can also be used as a supplement to existing feature parameters to improve the accuracy of REID as it is more insensitive to the signal-to-noise ratio (SNR) and signal modulation type changes.
Numerical Investigation of the Dynamic Performance and Riding Comfort of a Straddle-Type Monorail Subjected to Moving Trains
Authors: Gao Qingfei , Cui Kemeng , Li Zhonglong , Li Yan
Published: July 2020

The driving comfort of a straddle-type monorail, while considering the influence of the bridge structure, was studied on the basis of multibody dynamics and the finite element method. In this study, the coupled vehicle-bridge model was established through SIMPACK and ANSYS; the 3D model of the bridge was established in ANSYS, and the vehicle model with 35 degrees of freedom (DOFs) was established in SIMPACK. The influence of the vehicle speed, pier height, track irregularity, and vehicle load on riding comfort was studied. Overall, straddle-type monorails had a good running stability, and the lateral comfort of the vehicle was better than the vertical comfort, due to symmetrical horizontal wheels. As the vehicle speed increased, the acceleration of the bridge and vehicle increased accordingly. Track irregularity had a substantial influence on riding comfort. Three types of track irregularity were simulated, and this factor should be strictly controlled to be smoother than the Chinese national A-level road roughness. The bridge pier height had a notable influence on the lateral riding comfort. In addition, this study attempted to improve riding comfort from the perspective of increasing the bridge stiffness, which could be achieved by increasing the cross-beam thickness or the track beam height.
Energy-Based Prediction of the Displacement of DCFP Bearings
Authors: Li Jiaxi , Kishiki Shoichi , Yamada Satoshi , Yamazaki Shinsuke , Watanabe Atsushi , Terashima Masao
Published: July 2020

Isolation systems are currently being widely applied for earthquake resistance. During the design stage for such systems, the displacement response and input energy of the isolation layer are two of the main concerns. The prediction of these values is also of vital importance during the early stages of the structural design. In this study, the simple prediction method of double concave friction pendulum (DCFP) bearings is proposed, which can relate the response displacement of the isolation layer to the ground velocity through energy transfer with sufficient accuracy. Two friction models (the precise and simplified model) and a constant friction coefficient of double concave friction pendulum (DCFP) bearings are comprehensively validated by full-scale sinusoidal dynamic tests under various conditions. In addition, a response analysis, based on previous studies, was conducted using the friction models under selected unidirectional earthquake excitations, and the accuracy of using the simplified model in the response analysis was verified. Based on the response analysis data, this article verifies and optimizes the proposed prediction method by parameterizing the characteristics of earthquakes and combining the energy balance in order to gain a deeper understanding of the design of the isolation systems.
Microfluidic Quantification of Blood Pressure and Compliance Properties Using Velocity Fields under Periodic On–Off Blood Flows
Authors: Kang Yang Jun
Published: July 2020

To monitor variations of blood samples effectively, it is required to quantify static and dynamic properties simultaneously. With previous approaches, the viscosity and elasticity of blood samples are obtained for static and transient flows with two syringe pumps. In this study, simultaneous measurement of pressure and equivalent compliance is suggested by analyzing the velocity fields of blood flows, where a blood sample is delivered in a periodic on-off fashion with a single syringe pump. The microfluidic device is composed of a main channel (mc) for quantifying the equivalent compliance and a pressure channel (pc) for measuring the blood pressure. Based on the mathematical relation, blood pressure at junction (Px) is expressed as Px = kβ. Here, β is calculated by integrating the averaged velocity in the pressure channel (). The equivalent compliance (Ceq) is then quantified as Ceq = λoff · Q0/Px with a discrete fluidic model. The time constant (λoff ) is obtained from the transient behavior of the averaged blood velocity in the main channel (). According to results, Px and Ceq varied considerably with respect to the hematocrit and flow rate. The present method (i.e., blood pressure, compliance) shows a strong correlation with the previous method (i.e., blood viscosity, elasticity). In conclusion, the present method can be considered as a potential tool for monitoring the mechanical properties of blood samples supplied periodically from a single syringe pump.
Airborne Bacterial and Eukaryotic Community Structure across the United Kingdom Revealed by High-Throughput Sequencing
Authors: Song Hokyung , Crawford Ian , Lloyd Jonathan , Robinson Clare , Boothman Christopher , Bower Keith , Gallagher Martin , Allen Grant , Topping David
Published: July 2020

Primary biological aerosols often include allergenic and pathogenic microorganisms posing potential risks to human health. Moreover, there are airborne plant and animal pathogens that may have ecological and economic impact. In this study, we used high-throughput sequencing techniques (Illumina, MiSeq) targeting the 16S rRNA genes of bacteria and the 18S rRNA genes of eukaryotes, to characterize airborne primary biological aerosols. We used a filtration system on the UK Facility for Airborne Atmospheric Measurements (FAAM) research aircraft to sample a range of primary biological aerosols across southern England overflying surface measurement sites from Chilbolton to Weybourne. We identified 30 to 60 bacterial operational taxonomic units (OTUs) and 108 to 224 eukaryotic OTUs per sample. Moreover, 16S rRNA gene sequencing identified significant numbers of genera that have not been found in atmospheric samples previously or only been described in limited number of atmospheric field studies, which are rather old or published in local journals. This includes the genera Gordonia, Lautropia, and Psychroglaciecola. Some of the bacterial genera found in this study include potential human pathogens, for example, Gordonia, Sphingomonas, Chryseobacterium, Morganella, Fusobacterium, and Streptococcus. 18S rRNA gene sequencing showed Cladosporium to be the major genus in all of the samples, which is a well-known allergen and often found in the atmosphere. There were also genetic signatures of potentially allergenic taxa; for example, Pleosporales, Phoma, and Brassicales. Although there was no significant clustering of bacterial and eukaryotic communities depending on the sampling location, we found meteorological factors explaining significant variations in the community composition. The findings in this study support the application of DNA-based sequencing technologies for atmospheric science studies in combination with complementary spectroscopic and microscopic techniques for improved identification of primary biological aerosols.
Accuracy of Mean Radiant Temperature Derived from Active and Passive Radiometry
Authors: Staiger Henning , Matzarakis Andreas
Published: July 2020

The concept of the mean radiant temperature (Tmrt) allows the study of radiative exchanges between a human and its environment. It presupposes that the radiant effects on the person of the actual environment, which is generally heterogeneous, and the virtual environment, which is defined as homogeneous, are identical. ISO 7726 specifies the required accuracy in Tmrt as input of rational thermal indices, outdoors ±5 (K). Tmrt accounts for the radiant heat absorbed by skin/clothing from the shortwave (SW) and longwave (LW) spectral bands. Most of the radiant components are isotropic. However, there are anisotropic SW components; namely the direct irradiance and under clear or partly obstructed skies a significant circumsolar fraction (fcs) in the diffuse irradiance. Both originate from the close proximity of the solar disk. This study highlights the effect of fcs on Tmrt. In the scope of human biometeorology a standing body posture is standard. For unidirectional irradiances its radiant cross-section varies dependent on the solar altitude. Active radiometry in deriving Tmrt is based on measured irradiances. One method is the Klima-Michel-Modell (KMM) that uses readily available measurements from standard meteorologically radiant observations. KMM references Fanger’s area projection factors that are derived from precise measurements of real humans. Thus, KMM serves as reference in evaluation of further methods. One is the six-directional instrument (Tmrt,r,6−Dir). Slightly simplifying a standing human, it represents a subject as a rectangular solid. Tmrt,r,6−Dir is derived based on measured irradiances incident on the vertical and horizontal planes. In passive radiometry the energy balance equation of a black globe thermometer is solved that leads to Tmrt,Tg,BG. fcs significantly impacts Tmrt with noticeably reduced values for high and increased for low solar altitudes. Hence, accounting for fcs is essential for the accuracy of Tmrt. For KMM an extension to an existing algorithm is provided in order to include fcs into the Tmrt calculation that results in Tmrt,r,KMM. For Tmrt,r,6−Dir the radiant cross-section of the solid depends to a minor extent on its azimuth relative to the solar azimuth. As a result Tmrt,r,6−Dir slightly scatters compared to Tmrt,r,KMM. However, it remains within ±2 (K). Tmrt,Tg,BG compared to Tmrt,r,KMM complies only at night with the ISO 7726 bin of ±5 K. Tmrt,Tg,BG significantly overestimates Tmrt,r,KMM during the daytime, because of its greater SW absorptance compared to skin/clothing and to a smaller extent because the standing posture is represented by a sphere. Particularly in sunny conditions, Tmrt,Tg,BG is subject to considerable variance. Thus, outdoors during the daytime, Tmrt,Tg,BG is unable to serve as an appropriate input for the calculation of rational-based thermal indices.
Configuration–Interaction Perturbation Theory Calculations of Pu II
Authors: Savukov Igor
Published: July 2020

Configuration–interaction perturbation theory (CI–PT) is applied to calculations of low-energy states of Pu II. This ion is quite challenging due to a large number of possible determinants arising from seven valence electrons and strong relativistic effects. The CI–PT calculations agree with experiments for the energies and g-factors for many low-energy states that allowed positive identification of the theoretical levels. Isotope shifts were also used to aid in identification, and, in case of the odd states, fitting with three independent parameters was used to match theoretical isotope shifts to the experimental values with good accuracy. The CI–PT approach tested here on the Pu II ion can be generally used to calculate properties of many complex atoms, including U I that can find application in fundamental and applied science.
Attachable Hydrogel Containing Indocyanine Green for Selective Photothermal Therapy against Melanoma
Authors: Hwang Juyoung , Jin Jun-O
Published: July 2020

Melanoma is the most lethal form of skin cancer because it spreads easily to other tissues, thereby decreasing the efficiency of its treatment via chemo-, radio-, and surgical therapies. We suggest the application of an attachable hydrogel for the treatment of melanoma whereby the size and amount of incorporated indocyanine green (ICG) for photothermal therapy (PTT) can be controlled. An attachable hydrogel (poly(acrylamide-co-diallyldimethylammonium chloride); PAD) that incorporates ICG as a near-infrared (NIR) absorber was fabricated using a biocompatible polymer. The temperature of PAD-ICG increases under 808 nm laser irradiation. The hydrogel protects the ICG against decomposition; consequently, PAD-ICG can be reused for PTT. The attachment of PAD-ICG to an area with melanoma in mice, with irradiation using a NIR laser, successfully eliminated melanoma. Thus, the data suggest that PAD-ICG is a smart material that could be used for selective target therapy against melanoma in humans.
Clinical Significance of Electronegative Low-Density Lipoprotein Cholesterol in Atherothrombosis
Authors: Chu Chih-Sheng , Law Shi Hui , Lenzen David , Tan Yong-Hong , Weng Shih-Feng , Ito Etsuro , Wu Jung-Chou , Chen Chu-Huang , Chan Hua-Chen , Ke Liang-Yin
Published: July 2020

Despite the numerous risk factors for atherosclerotic cardiovascular diseases (ASCVD), cumulative evidence shows that electronegative low-density lipoprotein (L5 LDL) cholesterol is a promising biomarker. Its toxicity may contribute to atherothrombotic events. Notably, plasma L5 LDL levels positively correlate with the increasing severity of cardiovascular diseases. In contrast, traditional markers such as LDL-cholesterol and triglyceride are the therapeutic goals in secondary prevention for ASCVD, but that is controversial in primary prevention for patients with low risk. In this review, we point out the clinical significance and pathophysiological mechanisms of L5 LDL, and the clinical applications of L5 LDL levels in ASCVD can be confidently addressed. Based on the previously defined cut-off value by receiver operating characteristic curve, the acceptable physiological range of L5 concentration is proposed to be below 1.7 mg/dL. When L5 LDL level surpass this threshold, clinically relevant ASCVD might be present, and further exams such as carotid intima-media thickness, pulse wave velocity, exercise stress test, or multidetector computed tomography are required. Notably, the ultimate goal of L5 LDL concentration is lower than 1.7 mg/dL. Instead, with L5 LDL greater than 1.7 mg/dL, lipid-lowering treatment may be required, including statin, ezetimibe or PCSK9 inhibitor, regardless of the low-density lipoprotein cholesterol (LDL-C) level. Since L5 LDL could be a promising biomarker, we propose that a high throughput, clinically feasible methodology is urgently required not only for conducting a prospective, large population study but for developing therapeutics strategies to decrease L5 LDL in the blood.
Delayed Ischemic Neurological Deficit after Uneventful Elective Clipping of Unruptured Intracranial Aneurysms
Authors: Vachata Petr , Lodin Jan , Hejčl Aleš , Cihlář Filip , Sameš Martin
Published: July 2020

Cerebral vasospasm and subsequent delayed ischemic neurological deficit is a typical sequela of acute subarachnoid hemorrhage after aneurysm rupture. The occurrence of vasospasms after uncomplicated surgery of an unruptured aneurysm without history of suspected rupture is extremely rare. The pathogenesis and severity of cerebral vasospasms is typically correlated with the amount of blood breakdown products extravasated during subarachnoid hemorrhage. In rare cases, where vasospasms occur after unruptured aneurysm surgery, the pathogenesis is most likely multifactorial and unclear. We present two cases of vasospasms following uncomplicated clipping of middle cerebral artery (MCA) aneurysms and a review of literature. Early diagnosis and therapy of this rare complication are necessary to achieve optimal clinical outcomes.
Alexithymia Profile in Relation to Negative Affect in Parents of Autistic and Typically Developing Young Children
Authors: Leonardi Elisa , Cerasa Antonio , Famà Francesca Isabella , Carrozza Cristina , Spadaro Letteria , Scifo Renato , Baieli Sabrina , Marino Flavia , Tartarisco Gennaro , Vagni David , Pioggia Giovanni , Ruta Liliana
Published: July 2020

In our study, we explored the construct of alexithymia in parents of children with and without ASD using a multi-method approach based on self-rated and external rater assessment. We also assessed the level of self-report measures of negative affect states such as trait anxiety and depression, and investigated the correlation between the alexithymia construct, trait anxiety, and depression within the broader autism phenotype (BAP). A total sample of 100 parents (25 mothers and 25 fathers in each group) were administered the TAS-20 and the TSIA to measure self-reported and observer-rated alexithymia traits, as well as self-report measures of anxiety and depression. Study results showed that the TSIA but not the TAS-20 was able to detect significant group differences in alexithymia traits among parents of children with and without ASD, with parents of ASD children displaying significantly higher levels of alexithymia. Furthermore, differently from the TAS-20, no significant correlations between the TSIA and measures of anxiety and depression were detected. Taken together, our results suggest the importance of using multi-method approaches to control for potential measurement bias and to detect psychological constructs such as alexithymia in subclinical samples such as parents of children with ASD.
Frontal Cortical Modulation of Temporal Visual Cross-Modal Re-organization in Adults with Hearing Loss
Authors: Campbell Julia , Sharma Anu
Published: July 2020

Recent research has demonstrated frontal cortical involvement to co-occur with visual re-organization, suggestive of top-down modulation of cross-modal mechanisms. However, it is unclear whether top-down modulation of visual re-organization takes place in mild hearing loss, or is dependent upon greater degrees of hearing loss severity. Thus, the purpose of this study was to determine if frontal top-down modulation of visual cross-modal re-organization increased across hearing loss severity. We recorded visual evoked potentials (VEPs) in response to apparent motion stimuli in 17 adults with mild-moderate hearing loss using 128-channel high-density electroencephalography (EEG). Current density reconstructions (CDRs) were generated using sLORETA to visualize VEP generators in both groups. VEP latency and amplitude in frontal regions of interest (ROIs) were compared between groups and correlated with auditory behavioral measures. Activation of frontal networks in response to visual stimulation increased across mild to moderate hearing loss, with simultaneous activation of the temporal cortex. In addition, group differences in VEP latency and amplitude correlated with auditory behavioral measures. Overall, these findings support the hypothesis that frontal top-down modulation of visual cross-modal re-organization is dependent upon hearing loss severity.
Evidence for Glass Behavior in Amorphous Carbon
Authors: Best Steven , Wasley Jake B. , de Tomas Carla , Aghajamali Alireza , Suarez-Martinez Irene , Marks Nigel A.
Published: July 2020

Amorphous carbons are disordered carbons with densities of circa 1.9–3.1 g/cc and a mixture of sp2 and sp3 hybridization. Using molecular dynamics simulations, we simulate diffusion in amorphous carbons at different densities and temperatures to investigate the transition between amorphous carbon and the liquid state. Arrhenius plots of the self-diffusion coefficient clearly demonstrate that there is a glass transition rather than a melting point. We consider five common carbon potentials (Tersoff, REBO-II, AIREBO, ReaxFF and EDIP) and all exhibit a glass transition. Although the glass-transition temperature (Tg) is not significantly affected by density, the choice of potential can vary Tg by up to 40%. Our results suggest that amorphous carbon should be interpreted as a glass rather than a solid.
Comprehensive Analysis of LincRNAs in Classical and Basal-Like Subtypes of Pancreatic Cancer
Authors: Glaß Markus , Dorn Agnes , Hüttelmaier Stefan , Haemmerle Monika , Gutschner Tony
Published: July 2020

Pancreatic ductal adenocarcinomas (PDAC) belong to the deadliest malignancies in the western world. Mutations in TP53 and KRAS genes along with some other frequent polymorphisms occur almost universally and are major drivers of tumour initiation. However, these mutations cannot explain the heterogeneity in therapeutic responses and differences in overall survival observed in PDAC patients. Thus, recent classifications of PDAC tumour samples have leveraged transcriptome-wide gene expression data to account for epigenetic, transcriptional and post-transcriptional mechanisms that may contribute to this deadly disease. Intriguingly, long intervening RNAs (lincRNAs) are a special class of long non-coding RNAs (lncRNAs) that can control gene expression programs on multiple levels thereby contributing to cancer progression. However, their subtype-specific expression and function as well as molecular interactions in PDAC are not fully understood yet. In this study, we systematically investigated the expression of lincRNAs in pancreatic cancer and its molecular subtypes using publicly available data from large-scale studies. We identified 27 deregulated lincRNAs that showed a significant different expression pattern in PDAC subtypes suggesting context-dependent roles. We further analyzed these lincRNAs regarding their common expression patterns. Moreover, we inferred clues on their functions based on correlation analyses and predicted interactions with RNA-binding proteins, microRNAs, and mRNAs. In summary, we identified several PDAC-associated lincRNAs of prognostic relevance and potential context-dependent functions and molecular interactions. Hence, our study provides a valuable resource for future investigations to decipher the role of lincRNAs in pancreatic cancer.
Study of the Female Sex Survival Advantage in Melanoma—A Focus on X-Linked Epigenetic Regulators and Immune Responses in Two Cohorts
Authors: Emran Abdullah Al , Nsengimana Jérémie , Punnia-Moorthy Gaya , Schmitz Ulf , Gallagher Stuart J. , Newton-Bishop Julia , Tiffen Jessamy C. , Hersey Peter
Published: July 2020

Background: Survival from melanoma is strongly related to patient sex, with females having a survival rate almost twice that of males. Many explanations have been proposed but have not withstood critical scrutiny. Prior analysis of different cancers with a sex bias has identified six X-linked genes that escape X chromosome inactivation in females and are, therefore, potentially involved in sex differences in survival. Four of the genes are well-known epigenetic regulators that are known to influence the expression of hundreds of other genes and signaling pathways in cancer. Methods: Survival and interaction analysis were performed on the skin cutaneous melanoma (SKCM) cohort in The Cancer Genome Atlas (TCGA), comparing high vs. low expression of KDM6A, ATRX, KDM5C, and DDX3X. The Leeds melanoma cohort (LMC) on 678 patients with primary melanoma was used as a validation cohort. Results: Analysis of TCGA data revealed that two of these genes—KDM6A and ATRX—were associated with improved survival from melanoma. Tumoral KDM6A was expressed at higher levels in females and was associated with inferred lymphoid infiltration into melanoma. Gene set analysis of high KDM6A showed strong associations with immune responses and downregulation of genes associated with Myc and other oncogenic pathways. The LMC analysis confirmed the prognostic significance of KDM6A and its interaction with EZH2 but also revealed the expression of KDM5C and DDX3X to be prognostically significant. The analysis also confirmed a partial correlation of KDM6A with immune tumor infiltrates. Conclusion: When considered together, the results from these two series are consistent with the involvement of X-linked epigenetic regulators in the improved survival of females from melanoma. The identification of gene signatures associated with their expression presents insights into the development of new treatment initiatives but provides a basis for exploration in future studies.
Computational Identification of Gene Networks as a Biomarker of Neuroblastoma Risk
Authors: Sun Lidan , Jiang Libo , Grant Christa N. , Wang Hong-Gang , Gragnoli Claudia , Liu Zhenqiu , Wu Rongling
Published: July 2020

Neuroblastoma is a common cancer in children, affected by a number of genes that interact with each other through intricate but coordinated networks. Traditional approaches can only reconstruct a single regulatory network that is topologically not informative enough to explain the complexity of neuroblastoma risk. We implemented and modified an advanced model for recovering informative, omnidirectional, dynamic, and personalized networks (idopNetworks) from static gene expression data for neuroblastoma risk. We analyzed 3439 immune genes of neuroblastoma for 217 high-risk patients and 30 low-risk patients by which to reconstruct large patient-specific idopNetworks. By converting these networks into risk-specific representations, we found that the shift in patients from a low to high risk or from a high to low risk might be due to the reciprocal change of hub regulators. By altering the directions of regulation exerted by these hubs, it may be possible to reduce a high risk to a low risk. Results from a holistic, systems-oriented paradigm through idopNetworks can potentially enable oncologists to experimentally identify the biomarkers of neuroblastoma and other cancers.
Innovative CAR-T Cell Therapy for Solid Tumor; Current Duel between CAR-T Spear and Tumor Shield
Authors: Jo Yuna , Ali Laraib Amir , Shim Ju A , Lee Byung Ha , Hong Changwan
Published: July 2020

Novel engineered T cells containing chimeric antigen receptors (CAR-T cells) that combine the benefits of antigen recognition and T cell response have been developed, and their effect in the anti-tumor immunotherapy of patients with relapsed/refractory leukemia has been dramatic. Thus, CAR-T cell immunotherapy is rapidly emerging as a new therapy. However, it has limitations that prevent consistency in therapeutic effects in solid tumors, which accounts for over 90% of all cancer patients. Here, we review the literature regarding various obstacles to CAR-T cell immunotherapy for solid tumors, including those that cause CAR-T cell dysfunction in the immunosuppressive tumor microenvironment, such as reactive oxygen species, pH, O2, immunosuppressive cells, cytokines, and metabolites, as well as those that impair cell trafficking into the tumor microenvironment. Next-generation CAR-T cell therapy is currently undergoing clinical trials to overcome these challenges. Therefore, novel approaches to address the challenges faced by CAR-T cell immunotherapy in solid tumors are also discussed here.
DNA Damage-Inducing Anticancer Therapies: From Global to Precision Damage
Authors: Reuvers Thom G. A. , Kanaar Roland , Nonnekens Julie
Published: July 2020

DNA damage-inducing therapies are of tremendous value for cancer treatment and function by the direct or indirect formation of DNA lesions and subsequent inhibition of cellular proliferation. Of central importance in the cellular response to therapy-induced DNA damage is the DNA damage response (DDR), a protein network guiding both DNA damage repair and the induction of cancer-eradicating mechanisms such as apoptosis. A detailed understanding of DNA damage induction and the DDR has greatly improved our knowledge of the classical DNA damage-inducing therapies, radiotherapy and cytotoxic chemotherapy, and has paved the way for rational improvement of these treatments. Moreover, compounds targeting specific DDR proteins, selectively impairing DNA damage repair in cancer cells, form a promising novel therapy class that is now entering the clinic. In this review, we give an overview of the current state and ongoing developments, and discuss potential avenues for improvement for DNA damage-inducing therapies, with a central focus on the role of the DDR in therapy response, toxicity and resistance. Furthermore, we describe the relevance of using combination regimens containing DNA damage-inducing therapies and how they can be utilized to potentiate other anticancer strategies such as immunotherapy.
Episodic Breathlessness with and without Background Dyspnea in Advanced Cancer Patients Admitted to an Acute Supportive Care Unit
Authors: Mercadante Sebastiano , Adile Claudio , Ferrera Patrizia , Bonanno Giuseppe , Restivo Vincenzo , Casuccio Alessandra
Published: July 2020

Aim: To characterize episodic breathlessness (EB) in patients with advanced cancer, and to determine factors influencing its clinical appearance. Methods: A consecutive sample of advanced cancer patients admitted to an acute palliative care unit was surveyed. Continuous dyspnea and EB were measured by a numerical scale. The use of drugs used for continuous dyspnea and EB was recorded. Patients were asked about the characteristics of EB (frequency, intensity, duration and triggers). The Multidimensional dyspnea profile (MDP), the Brief dyspnea inventory (BDI), the Athens sleep scale (AIS) and the Hospital Anxiety and Depression Scale (HADS) were also administered. Results: From 439 advanced cancer patients surveyed, 34 and 27 patients had EB, without and with background dyspnea, respectively. The mean intensity and the number of episodes were higher in patients with background dyspnea (p < 0.0005 and p = 0.05, respectively). No differences in duration were observed. Most episodes lasted <10 min. A recognizable cause triggering EB was often found. The presence of both background dyspnea and EB was associated with higher values of MDP and BDI. EB was independently associated with frequency and intensity of background dyspnea (OR = 20.9, 95% CI (Confidence interval) 9.1–48.0; p < 0.0005 and OR = 1.97, 95% CI 1.09–3.58; p = 0.025, respectively) and a lower Karnofsky level (OR = 0.96, 95%CI 0.92–0.98, p = 0.05). Discussion: EB may occur in patients with and without continuous dyspnea, and is often induced by physical and psychological factors. EB intensity is higher in patients with continuous dyspnea. The duration was often so short that the use of drugs, as needed, may be too late, unless administered pre-emptively when the trigger was predictable.