bluuebunny/crossref_metadata_2025 huggingface dataset:
The Subthalamic Nucleus Influences Visuospatial Attention in Humans
Authors: Schmalbach Barbara , Günther Veronika , Raethjen Jan , Wailke Stefanie , Falk Daniela , Deuschl Günther , Witt Karsten
Published: October 2013

Spatial attention is a lateralized feature of the human brain. Whereas the role of cortical areas of the nondominant hemisphere on spatial attention has been investigated in detail, the impact of the BG, and more precisely the subthalamic nucleus, on signs and symptoms of spatial attention is not well understood. Here we used unilateral deep brain stimulation of the subthalamic nucleus to reversibly, specifically, and intraindividually modify the neuronal BG outflow and its consequences on signs and symptoms of visuospatial attention in patients suffering from Parkinson disease. We tested 13 patients with Parkinson disease and chronic deep brain stimulation in three stimulation settings: unilateral right and left deep brain stimulation of the subthalamic nucleus as well as bilateral deep brain stimulation of the subthalamic nucleus. In all three stimulation settings, the patients viewed a set of pictures while an eye-tracker system recorded eye movements. During the exploration of the visual stimuli, we analyzed the time spent in each visual hemispace, as well as the number, duration, amplitude, peak velocity, acceleration peak, and speed of saccades. In the unilateral left-sided stimulation setting, patients show a shorter ipsilateral exploration time of the extrapersonal space, whereas number, duration, and speed of saccades did not differ between the different stimulation settings. These results demonstrated reduced visuospatial attention toward the side contralateral to the right subthalamic nucleus that was not being stimulated in a unilateral left-sided stimulation. Turning on the right stimulator, the reduced visuospatial attention vanished. These results support the involvement of the subthalamic nucleus in modulating spatial attention. Therefore, the subthalamic nucleus is part of the subcortical network that subserves spatial attention.
Clutter Modulates the Representation of Target Objects in the Human Occipitotemporal Cortex
Authors: Erez Yaara , Yovel Galit
Published: October 2013

Target objects required for goal-directed behavior are typically embedded within multiple irrelevant objects that may interfere with their encoding. Most neuroimaging studies of high-level visual cortex have examined the representation of isolated objects, and therefore, little is known about how surrounding objects influence the neural representation of target objects. To investigate the effect of different types of clutter on the distributed responses to target objects in high-level visual areas, we used fMRI and manipulated the type of clutter. Specifically, target objects (i.e., a face and a house) were presented either in isolation, in the presence of a homogeneous (identical objects from another category) clutter (“pop-out” display), or in the presence of a heterogeneous (different objects) clutter, while participants performed a target identification task. Using multivoxel pattern analysis (MVPA) we found that in the posterior fusiform object area a heterogeneous but not homogeneous clutter interfered with decoding of the target objects. Furthermore, multivoxel patterns evoked by isolated objects were more similar to multivoxel patterns evoked by homogenous compared with heterogeneous clutter in the lateral occipital and posterior fusiform object areas. Interestingly, there was no effect of clutter on the neural representation of the target objects in their category-selective areas, such as the fusiform face area and the parahippocampal place area. Our findings show that the variation among irrelevant surrounding objects influences the neural representation of target objects in the object general area, but not in object category-selective cortex, where the representation of target objects is invariant to their surroundings.
Comparisons of Empirical Localization Techniques for Serial Ensemble Kalman Filters in a Simple Atmospheric General Circulation Model
Authors: Lei Lili , Anderson Jeffrey L.
Published: October 2013

Two techniques for estimating good localization functions for serial ensemble Kalman filters are compared in observing system simulation experiments (OSSEs) conducted with the dynamical core of an atmospheric general circulation model. The first technique, the global group filter (GGF), minimizes the root-mean-square (RMS) difference between the estimated regression coefficients using a hierarchical ensemble filter. The second, the empirical localization function (ELF), minimizes the RMS difference between the true values of the state variables and the posterior ensemble mean. Both techniques provide an estimate of the localization function for an observation’s impact on a state variable with few a priori assumptions about the localization function. The ELF localizations can have values larger than 1.0 at small distances, indicating that this technique addresses localization but also can correct the prior ensemble spread in the same way as a variance inflation when needed. OSSEs using ELF localizations generally have smaller root-mean-square error (RMSE) than the optimal Gaspari and Cohn (GC) localization function obtained by empirically tuning the GC width. The localization functions estimated by the GGF are broader than those from the ELF, and the OSSEs with the GGF localization generally have larger RMSE than the optimal GC localization function. The GGFs are too broad because of spurious correlation biases that occur in the OSSEs. These errors can be reduced by using a stochastic EnKF with perturbed observations instead of a deterministic EAKF.
Heteroscedastic Extended Logistic Regression for Postprocessing of Ensemble Guidance
Authors: Messner Jakob W. , Mayr Georg J. , Zeileis Achim , Wilks Daniel S.
Published: October 2013

To achieve well-calibrated probabilistic forecasts, ensemble forecasts are often statistically postprocessed. One recent ensemble-calibration method is extended logistic regression, which extends the popular logistic regression to yield full probability distribution forecasts. Although the purpose of this method is to postprocess ensemble forecasts, usually only the ensemble mean is used as the predictor variable, whereas the ensemble spread is neglected because it does not improve the forecasts. In this study it is shown that when simply used as an ordinary predictor variable in extended logistic regression, the ensemble spread affects the location but not the variance of the predictive distribution. Uncertainty information contained in the ensemble spread is therefore not utilized appropriately. To solve this drawback a new approach is proposed where the ensemble spread is directly used to predict the dispersion of the predictive distribution. With wind speed data and ensemble forecasts from the European Centre for Medium-Range Weather Forecasts (ECMWF) it is shown that by using this approach, the ensemble spread can be used effectively to improve forecasts from extended logistic regression.
What Happens to Artistic Aspirants Who Do Not "Succeed''? A Research Note From the Strategic National Arts Alumni Project
Authors: Lindemann Danielle J.
Published: October 2013

Scholars have often portrayed the arts as a winner-take-all market. However, data from the Strategic National Arts Alumni Project suggest that many artistic aspirants who do not work within the arts still incorporate artistic elements within their diverse careers and often engage in art avocationally. Further, these individuals continue to mobilize the skills and human capital involved in arts training in diverse realms. There are compelling reasons to examine the careers of the high numbers of aspiring artists who do not earn money in this capacity, and to pay attention to their alternative ways of putting together creative careers.
Mobile Project Networks: Regional Dynamics in the U.S. Film and Television Industry
Authors: Foster Pacey C.
Published: October 2013

Labor mobility and flexible employment practices have been recognized as central constructs in research on creative industries. However, despite the increasing mobility of creative work, flexible employment practices have received much more attention than the geographic mobility of creative projects and workers. This article addresses this gap by studying regional network dynamics in the evolution of the film industry in Massachusetts between 2000 and 2010. The author shows that the geographic distribution and specific types of labor and nonwage spending in the Massachusetts film industry varied over time as a function of the regional project mix and policies designed to lure highly mobile projects to the region.
Use of Medicaid 1915(c) Home- and Community-Based Care Waivers to Reconfigure State Long-Term Care Systems
Authors: Miller Nancy A. , Ramsland Sarah , Goldstein Elizabeth , Harrington Charlene
Published: October 2013

Since Congressional authorization in 1981, Medicaid 1915(c) home- and community-based care waivers have influenced states’ efforts to transform their long-term care systems. In 1997, every state participated in the 1915(c) waiver program, while waiver expenditures, at $8.1 billion, represented 59.6 percent of all Medicaid community-based care expenditures. To explore state-level factors that appear related to these expenditures, the authors turn to a body of work on Medicaid resource allocation. They compare the influence of five factors—sociodemographic, supply, economic, programmatic, and political environment—on states’ allocations to long-term care expenditures and 1915(c) waiver expenditures. The state economic environment was an important influence on total, as well as waiver expenditures. State regulation of long-term care supply demonstrated the most substantive relationship, increasing the share of dollars supporting 1915(c) waivers from 11.6 to 20.0 over the study period, all else equal.
Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)
Authors: Chen Zhongyuan , Gui Jianfang , Gao Xiaochan , Pei Chao , Hong Yijiang , Zhang Qiya
Published: October 2013

Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses.
Quantification of the impact of PSI:Biology according to the annotations of the determined structures
Authors: DePietro Paul J , Julfayev Elchin S , McLaughlin William A
Published: October 2013

Background

Protein Structure Initiative:Biology (PSI:Biology) is the third phase of PSI where protein structures are determined in high-throughput to characterize their biological functions. The transition to the third phase entailed the formation of PSI:Biology Partnerships which are composed of structural genomics centers and biomedical science laboratories. We present a method to examine the impact of protein structures determined under the auspices of PSI:Biology by measuring their rates of annotations. The mean numbers of annotations per structure and per residue are examined. These are designed to provide measures of the amount of structure to function connections that can be leveraged from each structure.

Results

One result is that PSI:Biology structures are found to have a higher rate of annotations than structures determined during the first two phases of PSI. A second result is that the subset of PSI:Biology structures determined through PSI:Biology Partnerships have a higher rate of annotations than those determined exclusive of those partnerships. Both results hold when the annotation rates are examined either at the level of the entire protein or for annotations that are known to fall at specific residues within the portion of the protein that has a determined structure.

Conclusions

We conclude that PSI:Biology determines structures that are estimated to have a higher degree of biomedical interest than those determined during the first two phases of PSI based on a broad array of biomedical annotations. For the PSI:Biology Partnerships, we see that there is an associated added value that represents part of the progress toward the goals of PSI:Biology. We interpret the added value to mean that team-based structural biology projects that utilize the expertise and technologies of structural genomics centers together with biological laboratories in the community are conducted in a synergistic manner. We show that the annotation rates can be used in conjunction with established metrics, i.e. the numbers of structures and impact of publication records, to monitor the progress of PSI:Biology towards its goals of examining structure to function connections of high biomedical relevance. The metric provides an objective means to quantify the overall impact of PSI:Biology as it uses biomedical annotations from external sources.
Attenuating effect of standardized fruit extract of punica granatum L in rat model of tibial and sural nerve transection induced neuropathic pain
Authors: Jain Vivek , Pareek Ashutosh , Bhardwaj Yashumati Ratan , Singh Nirmal
Published: October 2013

Background

Injury to a nerve is the most common reason of acquired peripheral neuropathy. Therefore, searching for effective substance to recover of nerve after injury is need of present era. The current study investigates the protective potential of Standardized Fruit Extract of Punica granatum L (PFE) [Ellagic acid (41.6%), Punicalagins (10%), Granatin (5.1%)] in Tibial & Sural Nerve Transection (TST) induced neuropathic pain in rats.

Methods

TST was performed by sectioning tibial and sural nerve portions of the sciatic nerve and leaving the common peroneal nerve intact. Acetone drop, pin-prick, hot plate, paint brush & Walking Track tests were performed to assess cold allodynia; mechanical heat, hyperalgesia and dynamic mechanical allodynia & tibial functional index respectively. The levels of TNF-α, TBARS, GSH and Nitrite were measured in the sciatic nerve as an index of inflammation & oxidative stress.

Results

TST led to significant development of cold allodynia; mechanical and heat hyperalgesia; dynamic mechanical allodynia; functional deficit in walking along with rise in the levels of TBARS, TNF-α, GSH and Nitrite. Administrations of PFE (100 & 300 mg/kg oral), significantly attenuate TST induced behavioral & biochemical changes. Pretreatments of BADGE (120 mg/kg IP) a PPAR-γ antagonist and nitric oxide precursor L-arginine (100 mg/kg IP) abolished the protective effect of PFE. Whereas, pretreatment of L-NAME (5 mg/kg IP) a NOS inhibitor significantly potentiated PFE’s protective effect of PFE.

Conclusion

PFE shown to have attenuating effect in TST induced neuropathic pain which may be attributed to potential PPAR-gamma agonistic activity, nitric oxide inhibitory, anti-inflammatory and anti oxidative actions.
Regulation of stem cell self-renewal and differentiation by Wnt and Notch are conserved throughout the adenoma-carcinoma sequence in the colon
Authors: Prasetyanti Pramudita Ramadhina , Zimberlin Cheryl Doreen , Bots Michael , Vermeulen Louis , De Sousa E Melo Felipe , Medema Jan Paul
Published: October 2013

Background

Colon cancer stem cells are shown to be the self-renewing cells within a tumor that give rise to all lineages of more differentiated tumor cells. In this respect they are remarkably similar to their non-malignant counterparts that orchestrate the intestinal lining. This suggests that, despite the numerous genetic aberrations and morphological changes that have occurred during cancer initiation and progression, a remnant homeostatic regulation persists.

Findings

Using a number of human and mouse intestinal-derived organoid cultures from normal, adenoma and cancerous tissues, we show here that Notch signals coordinate self-renewal and lineage determination not only in normal, but also at the adenoma and carcinoma stage in both mice and humans. Moreover, the Wnt pathway, which carries activating mutations in virtually all colon cancers, is not as previously predicted constitutively active in adenomas and carcinomas, but still displays a heterogeneous activity pattern that determined stemness in all stages of disease.

Conclusion

These data for the first time provide a comprehensive overview of Wnt and Notch-mediated signaling in the different stages of the adenoma-carcinoma sequence and demonstrates that these morphogenic pathways, despite mutations, remain crucial determinants of both architecture and hierarchy in normal and malignant intestinal tissue.
The Campylobacter jejuniCiaD effector protein activates MAP kinase signaling pathways and is required for the development of disease
Authors: Samuelson Derrick R , Eucker Tyson P , Bell Julia A , Dybas Leslie , Mansfield Linda S , Konkel Michael E
Published: October 2013

Background

Enteric pathogens utilize a distinct set of proteins to modulate host cell signaling events that promote host cell invasion, induction of the inflammatory response, and intracellular survival. Human infection with Campylobacter jejuni, the causative agent of campylobacteriosis, is characterized by diarrhea containing blood and leukocytes. The clinical presentation of acute disease, which is consistent with cellular invasion, requires the delivery of the Campylobacter invasion antigens (Cia) to the cytosol of host cells via a flagellar Type III Secretion System (T3SS). We identified a novel T3SS effector protein, which we termed CiaD that is exported from the C. jejuni flagellum and delivered to the cytosol of host cells.

Results

We show that the host cell kinases p38 and Erk 1/2 are activated by CiaD, resulting in the secretion of interleukin-8 (IL-8) from host cells. Additional experiments revealed that CiaD-mediated activation of p38 and Erk 1/2 are required for maximal invasion of host cells by C. jejuni. CiaD contributes to disease, as evidenced by infection of IL-10 knockout mice. Noteworthy is that CiaD contains a Mitogen-activated protein (MAP) kinase-docking site that is found within effector proteins produced by other enteric pathogens. These findings indicate that C. jejuni activates the MAP kinase signaling pathways Erk 1/2 and p38 to promote cellular invasion and the release of the IL-8 pro-inflammatory chemokine.

Conclusions

The identification of a novel T3SS effector protein from C. jejuni significantly expands the knowledge of virulence proteins associated with C. jejuni pathogenesis and provides greater insight into the mechanism utilized by C. jejuni to invade host cells.
Correlation of Nav1.8 and Nav1.9 Sodium Channel Expression with Neuropathic Pain in Human Subjects with Lingual Nerve Neuromas
Authors: Bird Emma V , Christmas Claire R , Loescher Alison R , Smith Keith G , Robinson Peter P , Black Joel A , Waxman Stephen G , Boissonade Fiona M
Published: October 2013

Background

Voltage-gated sodium channels Nav1.8 and Nav1.9 are expressed preferentially in small diameter sensory neurons, and are thought to play a role in the generation of ectopic activity in neuronal cell bodies and/or their axons following peripheral nerve injury. The expression of Nav1.8 and Nav1.9 has been quantified in human lingual nerves that have been previously injured inadvertently during lower third molar removal, and any correlation between the expression of these ion channels and the presence or absence of dysaesthesia investigated.

Results

Immunohistochemical processing and quantitative image analysis revealed that Nav1.8 and Nav1.9 were expressed in human lingual nerve neuromas from patients with or without symptoms of dysaesthesia. The level of Nav1.8 expression was significantly higher in patients reporting pain compared with no pain, and a significant positive correlation was observed between levels of Nav1.8 expression and VAS scores for the symptom of tingling. No significant differences were recorded in the level of expression of Nav1.9 between patients with or without pain.

Conclusions

These results demonstrate that Nav1.8 and Nav1.9 are present in human lingual nerve neuromas, with significant correlations between the level of expression of Nav1.8 and symptoms of pain. These data provide further evidence that changes in expression of Nav1.8 are important in the development and/or maintenance of nerve injury-induced pain, and suggest that Nav1.8 may be a potential therapeutic target.
Biochemical and molecular heterogeneity among isolates of Yersinia ruckeri from rainbow trout (Oncorhynchus mykiss, Walbaum) in north west Germany
Authors: Huang Yidan , Runge Martin , Michael Geovana Brenner , Schwarz Stefan , Jung Arne , Steinhagen Dieter
Published: October 2013

Background

Enteric Redmouth Disease (ERM), caused by Yersinia ruckeri, is one of the most important infectious diseases in rainbow trout (Oncorhynchus mykiss) aquaculture in Europe. More recently, non-motile vaccine resistant isolates appear to have evolved and are causing disease problems throughout Europe, including Germany. The aim of this study was to analyse the variation of biochemical and molecular characteristics of Y. ruckeri isolates collected in north west Germany as a basis for strain differentiation. The isolates originated mainly from rainbow trout and were characterised by biochemical profiling, 16S rDNA sequencing, repetitive sequence-based PCRs, including (GTG)5-PCR, BOX-PCR, ERIC-PCR and REP-PCR, and pulsed-field gel electrophoresis (PFGE).

Results

In total, 83 isolates were characterised, including 48 isolates collected during a field study in north west Germany. All isolates were confirmed as Y. ruckeri by the API 20E system. Five isolates were additionally confirmed as Y. ruckeri by Y. ruckeri-specific PCR and 16S rDNA sequencing. Only 17 isolates hydrolyzed Tween 80/20. Sixty-six isolates (79.5%) were non-motile. Two different patterns were obtained by REP-PCR, five patterns by ERIC-PCR, four patterns by (GTG)5-PCR and three patterns by BOX-PCR. NotI-directed PFGE resulted in 17 patterns that differed from each other by 25–29 fragments. Isolates from the field study clustered together as PFGE type C. According to the results of API 20E, repetitive sequence-based PCRs and PFGE, these isolates could be subdivided into 27 different groups.

Conclusions

The detailed molecular and phenotypic characterisation scheme developed in this study could be used to help trace the dissemination of Y. ruckeri isolates, and thus may represent part of improved disease monitoring plans in the future.
Risk factors for intra-abdominal hypertension and abdominal compartment syndrome among adult intensive care unit patients: a systematic review and meta-analysis
Authors: Holodinsky Jessalyn K , Roberts Derek J , Ball Chad G , Blaser Annika Reintam , Starkopf Joel , Zygun David A , Stelfox Henry Thomas , Malbrain Manu L , Jaeschke Roman C , Kirkpatrick Andrew W
Published: October 2013

Introduction

Although intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) are associated with substantial morbidity and mortality among critically ill adults, it remains unknown if prevention or treatment of these conditions improves patient outcomes. We sought to identify evidence-based risk factors for IAH and ACS in order to guide identification of the source population for future IAH/ACS treatment trials and to stratify patients into risk groups based on prognosis.

Methods

We searched electronic bibliographic databases (MEDLINE, EMBASE, PubMed, and the Cochrane Database from 1950 until January 21, 2013) and reference lists of included articles for observational studies reporting risk factors for IAH or ACS among adult ICU patients. Identified risk factors were summarized using formal narrative synthesis techniques alongside a random effects meta-analysis.

Results

Among 1,224 citations identified, 14 studies enrolling 2,500 patients were included. The 38 identified risk factors for IAH and 24 for ACS could be clustered into three themes and eight subthemes. Large volume crystalloid resuscitation, the respiratory status of the patient, and shock/hypotension were common risk factors for IAH and ACS that transcended across presenting patient populations. Risk factors with pooled evidence supporting an increased risk for IAH among mixed ICU patients included obesity (four studies; odds ratio (OR) 5.10; 95% confidence interval (CI), 1.92 to 13.58), sepsis (two studies; OR 2.38; 95% CI, 1.34 to 4.23), abdominal surgery (four studies; OR 1.93; 95% CI, 1.30 to 2.85), ileus (two studies; OR 2.05; 95% CI, 1.40 to 2.98), and large volume fluid resuscitation (two studies; OR 2.17; 95% CI, 1.30 to 3.63). Among trauma and surgical patients, large volume crystalloid resuscitation and markers of shock/hypotension and metabolic derangement/organ failure were risk factors for IAH and ACS while increased disease severity scores and elevated creatinine were risk factors for ACS in severe acute pancreatitis patients.

Conclusions

Although several IAH/ACS risk factors transcend across presenting patient diagnoses, some appear specific to the population under study. As our findings were somewhat limited by included study methodology, the risk factors reported in this study should be considered candidate risk factors until confirmed by a large prospective multi-centre observational study.
Resolving the paradox for protein aggregation diseases: a common mechanism for aggregated proteins to initially attack membranes without needing aggregates
Authors: Qin Haina , Lim Liangzhong , Wei Yuanyuan , Gupta Garvita , Song Jianxing
Published: October 2013

Paradoxically, aggregation of specific proteins is characteristic of many human diseases and aging, yet aggregates have been found to be unnecessary for initiating pathogenesis. Here we determined the NMR topology and dynamics of a helical mutant in a membrane environment transformed from the 125-residue cytosolic all-β MSP by the ALS-causing P56S mutation. Unexpectedly, despite its low hydrophobicity, the P56S major sperm protein (MSP) domain becomes largely embedded in the membrane environment with high backbone rigidity. Furthermore it is composed of five helices with amphiphilicity comparable to those of the partly-soluble membrane toxin mellitin and α-synuclein causing Parkinson's disease. Consequently, the mechanism underlying this chameleon transformation becomes clear: by disrupting the specific tertiary interaction network stabilizing the native all-β MSP fold to release previously-locked amphiphilic segments, the P56S mutation acts to convert the classic MSP fold into a membrane-active protein that is fundamentally indistinguishable from mellitin and α-synuclein which are disordered in aqueous solution but spontaneously partition into membrane interfaces driven by hydrogen-bond energetics gained from forming α-helix in the membrane environments. As segments with high amphiphilicity exist in all proteins, our study successfully resolves the paradox by deciphering that the proteins with a higher tendency to aggregate have a stronger potential to partition into membranes through the same mechanism as α-synuclein to initially attack membranes to trigger pathogenesis without needing aggregates. This might represent the common first step for various kinds of aggregated proteins to trigger familiar, sporadic and aging diseases. Therefore the homeostasis of aggregated proteins in vivo is the central factor responsible for a variety of human diseases including aging. The number and degree of the membrane attacks by aggregated proteins may act as an endogenous clock to count down the aging process. Consequently, a key approach to fight against them is to develop strategies and agents to maintain or even enhance the functions of the degradation machineries.
Multisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry
Authors: Frey Olivier , Rothe Jörg , Heer Flavio , van der Wal Peter D. , de Rooij Nico F. , Hierlemann Andreas
Published: October 2013

A miniature device enabling parallel
Metalloid Sn clusters: properties and the novel synthesis via a disproportionation reaction of a monohalide
Authors: Schrenk Claudio , Schnepf Andreas
Published: October 2013

Metalloid cluster compounds of tin of the general formulae SnnRm with n>m (R=organic ligand), where beside ligand-bound tin atoms also “naked” tin atoms, that only bind to other tin atoms, are present, represent a novel class of cluster compounds in tin chemistry. As the “naked” tin atoms inside these clusters exhibit an oxidation state of 0, the average oxidation state of the tin atoms within such metalloid tin clusters is in between 0 and 1. Thus, these cluster compounds may be seen as intermediates on the way to the elemental state. Therefore, interesting properties are expected for these compounds, which might complement results from nanotechnology. During the last years, different syntheses of such novel cluster compounds have been introduced, leading to several metalloid tin cluster compounds, which exhibit new and partly unusual structure and bonding properties. In this review, recent results in this novel field of group 14 chemistry are discussed, whereby special attention is focused on the novel synthetic route applying a disproportionation reaction of metastable Sn(I) halides.
La geografía del caos
Authors: Sanginés Pedro Agustín Salmerón
Published: October 2013

En este ensayo se pretende aclarar el cambiante mapa político-militar de México durante el verano y el otoño de 1914. Se busca mostrar la fuerza, las posiciones y los recursos de los grupos revolucionarios durante el proceso de gestación de la ruptura que provocaría la guerra civil en noviembre de 1914, guerra que enfrentó a convencionistas contra constitucionalistas. Además, se intenta cruzar el mapa económico y demográfico con el mapa militar, así como recurrir a fuentes originales para hacer una nueva valoración de esa coyuntura, contraria a las tradicionalmente aceptadas.

The aim of this paper is to clarify the changing political and military map of Mexico during the summer and autumn of 1914. I intend to show the strength, positions and resources of the revolutionary groups during the emergence of the rupture that would cause civil war between convencionistas and constitutionalistas in November 1914. Furthermore, I attempt to cross over the economic and demographic map with the military map using original sources to make a new assessment of the situation, contrary to the one traditionally accepted.
The Subcommander and the Sardinian
Authors: Henck Nick
Published: October 2013

Numerous scholars have adopted a Gramscian framework when analyzing the Zapatistas and Subcommander Marcos. Some, however, have gone beyond the evidence, claiming that Marcos was influenced by Gramsci. Inspection of the Subcommander’s discourse provides no proof that he has been directly influenced by the Sardinian; instead, indigenous thought and practice appear more influential in having shaped Marcos’s political philosophy. Marcos’s independence from Gramsci distances him from other contemporary Central American guerrillas and has wider implications concerning the timing and extent of Gramsci’s diffusion in Mexico. Gramsci did, however, contribute to an atmosphere of “antiauthoritarian eclecticism” among the Latin American new left from which Marcos emerged.

Muchos estudiosos han adoptado el marco teórico gramsciano para analizar a los zapatistas y al Subcomandante Marcos. Pero algunos han pasado por alto la evidencia y afirman que Marcos recibió influencia de Gramsci. Al inspeccionar el discurso del Subcomandante no se encuentran pruebas de que haya recibido influencia directa del pensador de Sardinia; en cambio, el pensamiento y la práctica indígenas aparecen como influencias más notorias en la conformación de la filosofía política de Marcos. La independencia de este último respecto de Gramsci lo aparta de otras guerrillas contemporáneas centroamericanas y tiene implicaciones más amplias en lo que toca al momento y alcance de la difusión de Gramsci en México. Lo cierto es que éste contribuyó a una atmósfera de “eclecticismo antiautoritario” entre la nueva izquierda latinoamericana de la que surgió Marcos.
La historia encarnada, Llanto de Carmen Boullosa
Authors: Muñoz Elizabeth Cummins
Published: October 2013

En su novela Llanto, novelas imposibles (1992), Carmen Boullosa explora el uso del cuerpo como una herramienta epistemológica para indagar en las tensiones entre el discurso tradicional de la historia mexicana y la lógica literaria de la novela moderna. Desde su momento posmoderno, la autora reconoce que la historiografía tradicional ya no es fiable y quiere encontrar el significado perdido mediante la dramatización literaria del cuerpo histórico y su entorno físico. Al fracasar como novela histórica moderna, su proyecto cumple con un rito íntimo y personal en el que la ausencia del pasado indígena colectivo se asimila por un proceso de duelo individual que está firmemente localizado en el cuerpo y representado por el llanto.

In her novel Llanto, novelas imposibles (1992), Carmen Boullosa explores the body as an epistemological means to examine the tensions between the traditional discourse of Mexican history and the literary logic of the modern novel. From her postmodern stance, she recognizes that traditional historiography is no longer reliable and wants to recover meaning through a literary dramatization of the historical body and its physical environment. Her project fails as a modern historical novel, but undergoes an intimate and personal rite in which the absence of the collective indigenous past is assimilated through an individual grieving process firmly located in the body and represented by tears.
Divergence of Iron Metabolism in Wild Malaysian Yeast
Authors: Lee Hana N , Mostovoy Yulia , Hsu Tiffany Y , Chang Amanda H , Brem Rachel B
Published: October 2013

Comparative genomic studies have reported widespread variation in levels of gene expression within and between species. Using these data to infer organism-level trait divergence has proven to be a key challenge in the field. We have used a wild Malaysian population of S. cerevisiae as a test bed in the search to predict and validate trait differences based on observations of regulatory variation. Malaysian yeast, when cultured in standard medium, activated regulatory programs that protect cells from the toxic effects of high iron. Malaysian yeast also showed a hyperactive regulatory response during culture in the presence of excess iron and had a unique growth defect in conditions of high iron. Molecular validation experiments pinpointed the iron metabolism factors AFT1, CCC1, and YAP5 as contributors to these molecular and cellular phenotypes; in genome-scale sequence analyses, a suite of iron toxicity response genes showed evidence for rapid protein evolution in Malaysian yeast. Our findings support a model in which iron metabolism has diverged in Malaysian yeast as a consequence of a change in selective pressure, with Malaysian alleles shifting the dynamic range of iron response to low-iron concentrations and weakening resistance to extreme iron toxicity. By dissecting the iron scarcity specialist behavior of Malaysian yeast, our work highlights the power of expression divergence as a signpost for biologically and evolutionarily relevant variation at the organismal level. Interpreting the phenotypic relevance of gene expression variation is one of the primary challenges of modern genomics.
Genotype to Phenotype Maps: Multiple Input Abiotic Signals Combine to Produce Growth Effects via Attenuating Signaling Interactions in Maize
Authors: Makumburage G Buddhika , Richbourg H Lee , LaTorre Kalindi D , Capps Andrew , Chen Cuixen , Stapleton Ann E
Published: October 2013

The complexity of allele interactions constrains crop improvement and the prediction of disease susceptibility. Additive allele effects are the foundation for selection in animal and plant breeding, and complex genetic and environmental interactions contribute to inefficient detection of desirable loci. Manipulation and modeling of other sources of variation, such as environmental variables, have the potential to improve our prediction of phenotype from genotype. As an example of our approach to analysis of the network linking environmental input to alleles, we mapped the genetic architecture of single and combined abiotic stress responses in two maize mapping populations and compared the observed genetic architecture patterns to simple theoretical predictions. Comparisons of single and combined stress effects on growth and biomass traits exhibit patterns of allele effects that suggest attenuating interactions among physiological signaling steps in drought and ultraviolet radiation stress responses. The presence of attenuating interactions implies that shared QTL found in sets of environments could be used to group environment types and identify underlying environmental similarities, and that patterns of stress-dependent genetic architecture should be studied as a way to prioritize prebreeding populations. A better understanding of whole-plant interactor pathways and genetic architecture of multiple-input environmental signaling has the potential to improve the prediction of genomic value in plant breeding and crop modeling.
Mated Progeny Production Is a Biomarker of Aging inCaenorhabditis elegans
Authors: Pickett Christopher L , Dietrich Nicholas , Chen Junfang , Xiong Chengjie , Kornfeld Kerry
Published: October 2013

The relationships between reproduction and aging are important for understanding the mechanisms of aging and evaluating evolutionary theories of aging. To investigate the effects of progeny production on reproductive and somatic aging, we conducted longitudinal studies of Caenorhabditis elegans hermaphrodites. For mated wild-type animals that were not sperm limited and survived past the end of the reproductive period, high levels of cross-progeny production were positively correlated with delayed reproductive and somatic aging. In this group of animals, individuals that generated more cross progeny also reproduced and lived longer than individuals that generated fewer cross progeny. These results indicate that progeny production does not accelerate reproductive or somatic aging. This longitudinal study demonstrated that cumulative cross progeny production through day four is an early-stage biomarker that is a positive predictor of longevity. Furthermore, in mated animals, high levels of early cross progeny production were positively correlated with high levels of late cross progeny production, indicating that early progeny production does not accelerate reproductive aging. The relationships between progeny production and aging were further evaluated by comparing self-fertile hermaphrodites that generated relatively few self progeny with mated hermaphrodites that generated many cross progeny. The timing of age-related somatic degeneration was similar in these groups, suggesting progeny production does not accelerate somatic aging. These studies rigorously define relationships between progeny production, reproductive aging, and somatic aging and identify new biomarkers of C. elegans aging. These results indicate that some mechanisms or pathways control age-related degeneration of both reproductive and somatic tissues in C. elegans.
Regulation of Gene Expression inNeurospora crassawith a Copper Responsive Promoter
Authors: Lamb Teresa M , Vickery Justin , Bell-Pedersen Deborah
Published: October 2013

Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled by copper availability. Excess copper represses, and copper depletion, via the use of a copper chelator, activates expression. The kinetics of induction and repression of tcu-1 are rapid, and the effects are long lived. We constructed a plasmid carrying the bar gene (for glufosinate selection) fused to the tcu-1 promoter. This plasmid permits the generation of DNA fragments that can direct integration of Ptcu-1 into any desired locus. We use this strategy to integrate Ptcu-1 in front of wc-1, a circadian oscillator and photoreceptor gene. The addition of excess copper to the Ptcu-1::wc-1 strain phenocopies a Δwc-1 strain, and the addition of the copper chelator, bathocuproinedisulfonic acid, phenocopies a wc-1 overexpression strain. To test whether copper repression can recapitulate the loss of viability that an essential gene knockout causes, we placed Ptcu-1 upstream of the essential gene, hpt-1. The addition of excess copper drastically reduced the growth rate as expected. Thus, this strategy will be useful to probe the biological function of any N. crassa gene through controlled expression.
Diastrophic dysplasia: prenatal diagnosis and review of the literature
Authors: Honório Jonathan Celli , Bruns Rafael Frederico , Gründtner Luciana Fernandes , Raskin Salmo , Ferrari Lilian Pereira , Araujo Júnior Edward , Nardozza Luciano Marcondes Machado
Published: October 2013

CONTEXT Diastrophic dysplasia is a type of osteochondrodysplasia caused by homozygous mutation in the gene DTDST (diastrophic dysplasia sulfate transporter gene). Abnormalities occurring particularly in the skeletal and cartilaginous system are typical of the disease, which has an incidence of 1 in 100,000 live births. CASE REPORT The case of a pregnant woman, without any consanguineous relationship with her husband, whose fetus was diagnosed with skeletal dysplasia based on ultrasound findings and DNA tests, is described. An obstetric ultrasound scan produced in the 16th week of gestation revealed characteristics that guided the clinical diagnosis. Prominent among these characteristics were rhizomelia of the lower and upper limbs (shortening of the proximal portions) and mesomelia (shortening of the intermediate portions). Both upper limbs showed marked curvature, with the first finger of the upper limbs in abduction and clinodactyly of the fifth finger. Molecular analysis using the polymerase chain reaction (PCR) and gene sequencing detected mutations that had already been described in the literature for the gene DTDST, named c.862C > T and c.2147_2148insCT. Therefore, the fetus was a compound heterozygote, carrying two different mutations. CONCLUSIONS Prenatal diagnosis of this condition allowed a more realistic interpretation of the prognosis, and of the couple's reproductive future. This case report shows the contribution of molecular genetics towards the prenatal diagnosis, for which there are few descriptions in the literature.
Effect of Intermittent Kangaroo Mother Care on Weight Gain of Low Birth Weight Neonates With Delayed Weight Gain
Authors: Samra Nashwa M. , Taweel Amal El , Cadwell Karin
Published: October 2013

Objective: To evaluate intermittent Kangaroo Mother Care (KMC) with additional opportunities to breastfeed on weight gain of low birth weight (LBW) neonates with delayed weight gain. Methods: 40 LBW neonates were followed to see whether KMC with additional opportunities to breastfeed improved weight gain. Results: In the KMC group, the mean age of regaining birth weight was significantly less (15.68 vs. 24.56 days) and the average daily weight gain was significantly higher (22.09 vs. 10.39 g, p < .001) than controls. Conclusion: KMC with additional opportunities to breastfeed was found to be an effective intervention for LBWs with delayed weight gain and should be considered to be an effective strategy.
Childrearing Among Thai First-Time Teenage Mothers
Authors: Sriyasak Atcharawadee , Åkerlind Ingemar , Akhavan Sharareh
Published: October 2013

The aim of this study is to explore and describe the experiences of being a teenage mother and taking care of infants less than 6 months of age. Ten teenage mothers were interviewed. Latent content analysis was used to analyze interview transcripts with the teenage mothers. It was found that previous childrearing experiences and social support were important factors in determining how teenage mothers adapted to being a mother and how they practiced infant care. Becoming a mother created feelings of responsibility in the maternal role and led to affection toward their babies. Nevertheless, teenage mothers appreciated the help they received from their families and health-care providers. Instruction and assistance with infant care built self-confidence in the maternal role and in childrearing.
Generalized Aggressive Periodontitis Associated With a Plasma Cell Gingivitis Lesion: A Case Report and Non‐Surgical Treatment
Authors: Parashis Andreas O. , Vardas Emmanouil , Tosios Konstantinos
Published: October 2013

Introduction: Plasma cell gingivitis (PCG) is an unusual inflammatory condition characterized by dense, band‐like polyclonal plasmacytic infiltration of the lamina propria. Clinically, it appears as gingival enlargement with erythema and swelling of the attached and free gingiva and is not associated with any loss of attachment. The aim of this report is to present a rare case of severe generalized aggressive periodontitis (GAgP) associated with a PCG lesion that was successfully treated and maintained non‐surgically.

Case Presentation: A 32‐year‐old white male with a non‐contributory medical history presented with gingival enlargement with diffuse erythema and edematous swelling, predominantly around teeth #5 through #8. Clinical and radiographic examination revealed generalized severe periodontal breakdown. A complete blood count and biochemical tests were within normal limits. Histologic and immunohistochemical examination were consistent with PCG. A diagnosis of severe GAgP associated with a PCG lesion was assigned. Treatment included elimination of possible allergens and non‐surgical periodontal treatment in combination with azithromycin. Clinical examination at reevaluation revealed complete resolution of gingival enlargement, erythema, and edema and localized residual probing depths of 5 mm. One year after treatment, the clinical condition was stable. Radiographs indicated improved bone levels and formation of crestal lamina dura.

Conclusion: This case report highlights the unusual coexistence of GAgP and PCG, in which non‐surgical treatment with elimination of all possible causes in combination with antimicrobials resulted in elimination of the gingival enlargement and significant improvement of periodontal parameters.