bluuebunny/crossref_metadata_2025 huggingface dataset:
A Real-World Study on the Clinical Characteristics, Outcomes, and Relationship between Antibiotic Exposure and Clostridioides difficile Infection
Authors: Vintila Bogdan Ioan , Arseniu Anca Maria , Morgovan Claudiu , Butuca Anca , Bîrluțiu Victoria , Dobrea Carmen Maximiliana , Rus Luca Liviu , Ghibu Steliana , Bereanu Alina Simona , Arseniu Rares , Roxana Codru Ioana , Sava Mihai , Gabriela Gligor Felicia
Published: February 2024

Clostridioides difficile is a Gram-positive bacteria that causes nosocomial infections, significantly impacting public health. In the present study, we aimed to describe the clinical characteristics, outcomes, and relationship between antibiotic exposure and Clostridioides difficile infection (CDI) in patients based on reports from two databases. Thus, we conducted a retrospective study of patients diagnosed with CDI from Sibiu County Clinical Emergency Hospital (SCCEH), Romania, followed by a descriptive analysis based on spontaneous reports submitted to the EudraVigilance (EV) database. From 1 January to 31 December 2022, we included 111 hospitalized patients with CDI from SCCEH. Moreover, 249 individual case safety reports (ICSRs) from EVs were analyzed. According to the data collected from SCCEH, CDI was most frequently reported in patients aged 65–85 years (66.7%) and in females (55%). In total, 71.2% of all patients showed positive medical progress. Most cases were reported in the internal medicine (n = 30, 27%), general surgery (n = 26, 23.4%), and infectious disease (n = 22, 19.8%) departments. Patients were most frequently exposed to ceftriaxone (CFT) and meropenem (MER). Also, in the EV database, most CDI-related ADRs were reported for CFT, PIP/TAZ (piperacillin/tazobactam), MER, and CPX (ciprofloxacin). Understanding the association between previous antibiotic exposure and the risk of CDI may help update antibiotic stewardship protocols and reduce the incidence of CDI by lowering exposure to high-risk antibiotics.
The Role of Glutathione and Its Precursors in Type 2 Diabetes
Authors: Tuell Dawn , Ford George , Los Evan , Stone William
Published: February 2024

Type 2 diabetes (T2D) is a major worldwide health crisis affecting about 6.2% of the world’s population. Alarmingly, about one in five children in the USA have prediabetes. Glutathione (GSH) and its precursors play a promising role in the prevention and management of type T2D. Oxidative stress (OxS) is a probable factor in both T2D initiation and progression. GSH is the major cytosolic water-soluble chemical antioxidant and emerging evidence supports its role in improving T2D outcomes. Dietary supplementation with N-acetyl-cysteine (NAC) and/or glycine (GLY), which are GSH precursors, has also been studied for possible beneficial effects on T2D. This review will focus on the underlying pathophysiological and molecular mechanisms linking GSH and its precursors with T2D and OxS. In addition to their traditional antioxidant roles, the in vivo effects of GSH/NAC/GLY supplements will be evaluated for their potential abilities to modulate the complex pro-oxidant pathophysiological factors (e.g., hyperglycemia) driving T2D progression. Positive feedback loops that amplify OxS over long time intervals are likely to result in irreversible T2D micro- and macro-vascular damage. Most clinical studies with GSH/NAC/GLY have focused on adults or the elderly. Future research with pediatric populations should be a high priority since early intervention is critical.
A New Non-Obese Steatohepatitis Mouse Model with Cardiac Dysfunction Induced by Addition of Ethanol to a High-Fat/High-Cholesterol Diet
Authors: Shiraishi Seiji , Liu Jinyao , Saito Yuki , Oba Yumiko , Nishihara Yuiko , Yoshimura Satomichi
Published: February 2024

Non-obese metabolic dysfunction-associated steatotic liver disease (MASLD) has been associated with cardiovascular-related mortality, leading to a higher mortality rate compared to the general population. However, few reports have examined cardiovascular events in non-obese MASLD mouse models. In this study we created a mouse model to mimic this condition. In this study involving seven-week-old C57BL/6J male mice, two dietary conditions were tested: a standard high-fat/high-cholesterol diet (STHD-01) and a combined diet of STHD-01 and ethanol. Over periods of 6 and 12 weeks, we analyzed the effects on liver and cardiac tissues using various staining techniques and PCR. Echocardiography and blood tests were also performed to assess cardiac function and liver damage. The results showed that mice on the ethanol-supplemented STHD-01 diet developed signs of steatohepatitis and cardiac dysfunction, along with increased sympathetic activity, as early as 6 weeks. At 12 weeks, more pronounced exacerbations accompanied with cardiac dilation, advanced liver fibrosis, and activated myocardial fibrosis with sympathetic activation were observed. This mouse model effectively replicated non-obese MASLD and cardiac dysfunction over a 12-week period using a combined diet of STHD-01 and ethanol. This dietary approach highlighted that both liver inflammation and fibrosis, as well as cardiac dysfunction, could be significantly worsened due to the activation of the sympathetic nervous system. Our results indicate that alcohol, even when completely metabolized on the day of drinking, exacerbates the progression of non-obese MASLD and cardiac dysfunction.
Dynamic Leadership Mechanism in Homing Pigeon Flocks
Authors: Xie Lin , Zhang Xiangyin
Published: February 2024

In recent years, an increasing number of studies have focused on exploring the principles and mechanisms underlying the emergence of collective intelligence in biological populations, aiming to provide insights for human society and the engineering field. Pigeon flock behavior garners significant attention as a subject of study. Collective homing flight is a commonly observed behavioral pattern in pigeon flocks. The study analyzes GPS data during the homing process and utilizes acceleration information, which better reflects the flock’s movement tendencies during turns, to describe the leadership relationships within the group. By examining the evolution of acceleration during turning, the study unveils a dynamic leadership mechanism before and after turns, employing a more intricate dynamic model to depict the flock’s motion. Specifically, during stable flight, pigeon flocks tend to rely on fixed leaders to guide homing flight, whereas during turns, individuals positioned in the direction of the flock’s turn experience a notable increase in their leadership status. These findings suggest the existence of a dynamic leadership mechanism within pigeon flocks, enabling adaptability and stability under diverse flight conditions. From an engineering perspective, this leadership mechanism may offer novel insights for coordinating industrial multi-robot systems and controlling drone formations.
An Overview of Hydrogen Energy Generation
Authors: AlZohbi Gaydaa
Published: February 2024

The global issue of climate change caused by humans and its inextricable linkage to our present and future energy demand presents the biggest challenge facing our globe. Hydrogen has been introduced as a new renewable energy resource. It is envisaged to be a crucial vector in the vast low-carbon transition to mitigate climate change, minimize oil reliance, reinforce energy security, solve the intermittency of renewable energy resources, and ameliorate energy performance in the transportation sector by using it in energy storage, energy generation, and transport sectors. Many technologies have been developed to generate hydrogen. The current paper presents a review of the current and developing technologies to produce hydrogen from fossil fuels and alternative resources like water and biomass. The results showed that reformation and gasification are the most mature and used technologies. However, the weaknesses of these technologies include high energy consumption and high carbon emissions. Thermochemical water splitting, biohydrogen, and photo-electrolysis are long-term and clean technologies, but they require more technical development and cost reduction to implement reformation technologies efficiently and on a large scale. A combination of water electrolysis with renewable energy resources is an ecofriendly method. Since hydrogen is viewed as a considerable game-changer for future fuels, this paper also highlights the challenges facing hydrogen generation. Moreover, an economic analysis of the technologies used to generate hydrogen is carried out in this study.
The Impact of Qualification and Hospice Education on Staff Attitudes during Palliative Care in Pediatric Oncology Wards—A National Survey
Authors: Salamon Eszter , Fodor Éva , Földesi Enikő , Hauser Peter , Kriván Gergely , Csanádi Krisztina , Garami Miklós , Kovacs Gabor , Csóka Monika , Tiszlavicz Lilla Györgyi , Kiss Csongor , Dergez Tímea , Ottóffy Gábor
Published: February 2024

Background: Our knowledge about the attitudes of healthcare staff to palliative care in pediatric oncology is scarce. We aimed to assess their perceptions of palliative care in Hungary and find answers to the question of how to provide good palliative care for children. Method: Physicians (n = 30) and nurses (n = 43) working in the field of pediatric oncology (12 of them specialized in hospice care) were interviewed. Palliative care practice (communication, integration of palliative care, professionals’ feelings and attitudes, and opportunities for improvement) was assessed by semi-structured interviews evaluated in a mixed quantitative and qualitative way by narrative categorical content analysis and thematic analysis. Results: All providers displayed high negative emotions, positive evaluations, and used many active verbs. Nurses showed higher levels of denial, more self-references, and were more likely to highlight loss. Physicians emphasized the importance of communication regarding adequate or inadequate palliative care. Hospice specialists showed a higher passive verb rate, a lower self-reference, a lower need for psychological support, and a greater emphasis on teamwork and professional aspects. Conclusion: Our results show that nurses are more emotionally stressed than doctors in palliative care in pediatric oncology. To our knowledge, a study comparing doctors and nurses in this field has yet to be carried out. Our results suggest that pediatric oncological staff can positively evaluate a child’s palliative care despite the emotional strain. Regarding hospices, professional practice in palliative care may be a protective factor in reducing emotional distress and achieving professional well-being.
Role of Satisfaction with Life, Sex and Body Mass Index in Physical Literacy of Spanish Children
Authors: Urbano-Mairena Javier , Mendoza-Muñoz María , Carlos-Vivas Jorge , Pastor-Cisneros Raquel , Castillo-Paredes Antonio , Rodal Miguel , Muñoz-Bermejo Laura
Published: February 2024

Physical activity (PL) is essential to achieve good health, prevent cardiovascular diseases, obesity and overweight, as well as to achieve a better quality of life. Therefore, PL could become the tool to increase the practice of physical activity among young people, thus increasing life satisfaction (LS) given its positive relationship with physical activity. A single-measure cross-sectional correlational study was carried out, involving 135 children aged 8–12 years from Extremadura. They were administered the SWLS questionnaire and the Canadian assessment of physical literacy (CAPL-2). Significantly higher levels of PL (p = 0.010) were found in normal-weight children compared to overweight and obese children, due to the physical competence domain score (p < 0.001). PL was directly related to SWLS (p < 0.001), but inversely related to BMI (p = 0.018). The daily physical activity behaviour domain was explained by SWLS (p < 0.001) and sex (p < 0.001). Physical competence was described by SWLS (p < 0.001) and BMI (p = 0.045). Finally, the motivation and confidence domain were only significantly associated with SWLS (p < 0.001). It was concluded that boys and girls of normal weight achieved higher levels of PL and LS than those with overweight and obesity, establishing a negative relationship of PL with BMI and positive with LS.
Effect of Particle Size on the Physical Properties of PLA/Potato Peel Composites
Authors: Miller Katharina , Reichert Corina L. , Loeffler Myriam , Schmid Markus
Published: February 2024

In recent years, agricultural by-product fillers have been investigated in composites to influence the physical properties of the packaging material, increase biodegradability, and reduce costs. In general, the properties of composites are mainly influenced by the type, amount, and size of fillers. The aim of this study was to characterize potato peel particles as a filler in a poly(lactic acid) (PLA) matrix and to determine the effect of particle size on the physical properties of the composite. Therefore, different fractions of potato peel powder (0–53 μm, 125–250 μm, and 315–500 μm) were incorporated into PLA matrix via compounding and injection-molding. Microscopic analysis of the injection-molded samples revealed that the average particle shape did not differ between the different fractions. Overall, increasing the particle size of potato peel particles resulted in increased stiffness and decreased ductility. The cold crystallization temperature and water vapor transmission rate of the composites were independent of particle size but increased upon the incorporation of potato peel particles. In conclusion, the effect of particle incorporation on packaging-related properties was higher than the effect of using different particle size fractions. This means that potato peel particles, regardless of their particle size distribution, are promising fillers for composites, with the potential to improve biodegradability, maintain some level of protection for the packaged product, and reduce the cost of the composites.
Organ-On-A-Chip (OOC) Image Dataset for Machine Learning and Tissue Model Evaluation
Authors: Movčana Valērija , Strods Arnis , Narbute Karīna , Rūmnieks Fēlikss , Rimša Roberts , Mozoļevskis Gatis , Ivanovs Maksims , Kadiķis Roberts , Zviedris Kārlis Gustavs , Leja Laura , Zujeva Anastasija , Laimiņa Tamāra , Abols Arturs
Published: February 2024

Organ-on-a-chip (OOC) technology has emerged as a groundbreaking approach for emulating the physiological environment, revolutionizing biomedical research, drug development, and personalized medicine. OOC platforms offer more physiologically relevant microenvironments, enabling real-time monitoring of tissue, to develop functional tissue models. Imaging methods are the most common approach for daily monitoring of tissue development. Image-based machine learning serves as a valuable tool for enhancing and monitoring OOC models in real-time. This involves the classification of images generated through microscopy contributing to the refinement of model performance. This paper presents an image dataset, containing cell images generated from OOC setup with different cell types. There are 3072 images generated by an automated brightfield microscopy setup. For some images, parameters such as cell type, seeding density, time after seeding and flow rate are provided. These parameters along with predefined criteria can contribute to the evaluation of image quality and identification of potential artifacts. This dataset can be used as a basis for training machine learning classifiers for automated data analysis generated from an OOC setup providing more reliable tissue models, automated decision-making processes within the OOC framework and efficient research in the future.
Contribution of Social Media Addiction on Intention to Buy in Social Media Sites
Authors: Leite Ângela , Rodrigues Anabela , Ribeiro Ana Margarida , Lopes Sílvia
Published: February 2024

The aim of this study is to assess whether social media addiction contributes to the intention to buy; it is based on the model of Hajli (2014) that assesses the relationships between the constructs of social media use, trust, perceived usefulness, and intention to buy in social media sites. To this end, a confirmatory factor analysis was carried out to evaluate whether the Hajli model applied to this sample, as well as multigroup CFA to measure invariance across gender and across following influencers or not. Finally, the path analysis evaluates the intersection of social media addiction with the Hajli model (2014). The results confirmed the Hajli model as well as the inclusion in the model of social media addiction as a variable that contributes to purchase intention on social media. Configural, metric, and scalar invariance were found across genders and across the following influencers or not. Also, the values found for internal consistency and composite reliability, convergent reliability, and discriminant reliability were within the reference values.
A Novel Technique Based on Machine Learning for Detecting and Segmenting Trees in Very High Resolution Digital Images from Unmanned Aerial Vehicles
Authors: Kouvaras Loukas , Petropoulos George P.
Published: February 2024

The present study proposes a technique for automated tree crown detection and segmentation in digital images derived from unmanned aerial vehicles (UAVs) using a machine learning (ML) algorithm named Detectron2. The technique, which was developed in the python programming language, receives as input images with object boundary information. After training on sets of data, it is able to set its own object boundaries. In the present study, the algorithm was trained for tree crown detection and segmentation. The test bed consisted of UAV imagery of an agricultural field of tangerine trees in the city of Palermo in Sicily, Italy. The algorithm’s output was the accurate boundary of each tree. The output from the developed algorithm was compared against the results of tree boundary segmentation generated by the Support Vector Machine (SVM) supervised classifier, which has proven to be a very promising object segmentation method. The results from the two methods were compared with the most accurate yet time-consuming method, direct digitalization. For accuracy assessment purposes, the detected area efficiency, skipped area rate, and false area rate were estimated for both methods. The results showed that the Detectron2 algorithm is more efficient in segmenting the relevant data when compared to the SVM model in two out of the three indices. Specifically, the Detectron2 algorithm exhibited a 0.959% and 0.041% fidelity rate on the common detected and skipped area rate, respectively, when compared with the digitalization method. The SVM exhibited 0.902% and 0.097%, respectively. On the other hand, the SVM classification generated better false detected area results, with 0.035% accuracy, compared to the Detectron2 algorithm’s 0.056%. Having an accurate estimation of the tree boundaries from the Detectron2 algorithm, the tree health assessment was evaluated last. For this to happen, three different vegetation indices were produced (NDVI, GLI and VARI). All those indices showed tree health as average. All in all, the results demonstrated the ability of the technique to detect and segment trees from UAV imagery.
Hydraulic Flushing of Sediment in Reservoirs: Best Practices of Numerical Modeling
Authors: Lai Yong G. , Huang Jianchun , Greimann Blair P.
Published: February 2024

This article provides a comprehensive review and best practices for numerically simulating hydraulic flushing for reservoir sediment management. Three sediment flushing types are discussed: drawdown flushing, pressure flushing, and turbidity current venting. The need for reservoir sediment management and the current practices are reviewed. Different hydraulic drawdown types are described in terms of the basic physical processes involved as well as the empirical/analytical assessment tools that may be used. The primary focus has been on the numerical modeling of various hydraulic flushing options. Three model categories are reviewed: one-dimensional (1D), two-dimensional (2D) depth-averaged or layer-averaged, and three-dimensional (3D) computational fluid dynamics (CFD) models. General guidelines are provided on how to select a proper model given the characteristics of the reservoir and the flushing method, as well as specific guidelines for modeling. Case studies are also presented to illustrate the guidelines.
Experimental Studies on Vortex-Induced Vibration of a Piggyback Pipeline
Authors: Xiao Difei , Hao Zhiyong , Zhou Tongming , Zhu Hongjun
Published: February 2024

Offshore pipelines of different diameters are often seen in piggyback arrangements in close proximity. Under the effects of external flows, the pipelines may experience vibration. Reliable prediction of the vibration amplitudes is important for the design and operation of these structures. In the present study, the effect of the position angle (α) and gap ratio (G/D) of a piggyback pipeline on the amplitude of 1DOF vortex-induced vibration (VIV) was investigated experimentally in a wind tunnel. The diameter ratio d/D of the two cylinders was 0.5. Five position angles, namely, α = 0°, 45°, 90°, 135°, and 180°, and six gap ratios at each angle, G/D = 0, 0.1, 0.2, 0.3, 0.4, 0.5, were tested. It was found that both α and G/D affected the amplitude of vibrations significantly. For all gap ratios, the amplitude of vibrations increased from α = 0° to α = 90° and then decreased to a minimum value around α = 135°. The maximum amplitude occurred around α = 90° when G/D = 0, and the minimum occurred around α = 135°, when G/D = 0.2–0.3. At other position angles, the vibration amplitude was less sensitive to G/D, especially when the latter was between 0.1 and 0.4. These results verified those obtained using numerical methods and are invaluable to engineers when designing offshore piggyback pipelines.
Investigation on the Efficacy of Two Food Supplements Containing a Fixed Combination of Selected Probiotics and β-Glucans or Elderberry Extract for the Immune System: Modulation on Cytokines Expression in Human THP-1 and PBMC
Authors: Cappellucci Giorgio , Baini Giulia , Miraldi Elisabetta , Pauletto Lara , De Togni Heide , Raso Floriana , Biagi Marco
Published: February 2024

Several herbal and other natural products are used as ingredients in food supplements to strengthen immunity even if, very often, marketed products are proposed without a clear rationale or experimental evidence. In this study, we aimed to investigate the effect on human monocytes (THP-1) and on ex vivo human peripheral blood mononuclear cells (PBMC) of two formulations, one containing Bifidobacterium animalis subsp. lactis Bl-04® with β-glucans (for adults) and one containing Lactobacillus rhamnosus CRL1505 with elderberry extract (for children). We compared formulations with single ingredients, with bacterial lipopolysaccharide (LPS) and the drug pidotimod; cytokines expression level was evaluated testing different concentrations of samples at two exposure times. As expected, LPS caused a non-specific huge upregulation of cytokines expression both in THP-1 and in PBMC, whereas pidotimod mainly upregulated IL-2 in PBMC and IL-8 in THP-1. The two formulations showed a difference between a pro-inflammatory stimulus such as LPS, and also from an immunostimulant drug, such as pidotimod, as they mainly upregulated the expression of IL-6 and IL-10 in PBMC but not in THP-1, in a concentration-dependent mode. Probiotics were shown to play a major role, but β-glucans and elderberry extract exerted a synergistic activity. This work demonstrated that combining selected probiotics with other natural products having immunomodulatory properties is an interesting strategy to develop innovative formulations in the sector of food supplements.
Forecasting the Occurrence of Electricity Price Spikes: A Statistical-Economic Investigation Study
Authors: Zamudio López Manuel , Zareipour Hamidreza , Quashie Mike
Published: February 2024

This research proposes an investigative experiment employing binary classification for short-term electricity price spike forecasting. Numerical definitions for price spikes are derived from economic and statistical thresholds. The predictive task employs two tree-based machine learning classifiers and a deterministic point forecaster; a statistical regression model. Hyperparameters for the tree-based classifiers are optimized for statistical performance based on recall, precision, and F1-score. The deterministic forecaster is adapted from the literature on electricity price forecasting for the classification task. Additionally, one tree-based model prioritizes interpretability, generating decision rules that are subsequently utilized to produce price spike forecasts. For all models, we evaluate the final statistical and economic predictive performance. The interpretable model is analyzed for the trade-off between performance and interpretability. Numerical results highlight the significance of complementing statistical performance with economic assessment in electricity price spike forecasting. All experiments utilize data from Alberta’s electricity market.
Systematic Reviews and Synthesis without Meta-Analysis on Hydrotherapy for Pain Control in Labor
Authors: Mellado-García Elena , Díaz-Rodríguez Lourdes , Cortés-Martín Jonathan , Sánchez-García Juan Carlos , Piqueras-Sola Beatriz , Higuero Macías Juan Carlos , Rodríguez-Blanque Raquel
Published: February 2024

Background: Although there is scientific evidence regarding the use of water immersion during labor, this evidence is primarily focused on the first stage of labor. There is limited scientific evidence on water immersion during the second stage of labor. Objective: The objective of this study was to conduct a comprehensive systematic review and synthesis of contemporary evidence related to water birth, with a specific focus on the second stage of labor. Methods: A systematic review of the scientific literature published between January 2018 and October 2023 was carried out. A synthesis of the results was conducted following the Synthesis without Meta-Analysis (SWiM) guidelines. PubMed, Scopus, and the Cochrane Library were utilized as information sources. The search strategy was designed using the keywords “immersion” and “parturition”, along with their relevant synonyms. Inclusion criteria encompassed studies employing randomized controlled trials (RCTs), systematic reviews, and quantitative and qualitative approaches focusing on pregnant women undergoing water immersion at any stage of the labor process. Results: Eleven articles were selected: two systematic reviews (one quantitative and one qualitative), five cohort studies, one case–control study, one cross-sectional observational study, and two qualitative studies. A thorough assessment of the methodology was performed using several specific tools: the Cochrane RoB 2 (Risk of Bias 2) tool for systematic reviews, JBI Critical Appraisal Checklist for Qualitative Research for qualitative studies, STROBE for observational descriptive studies, and CASPe for qualitative studies. The results provided fundamental insights that will contribute to conceptual standardization regarding the effects of water birth on maternal and fetal health. Additionally, a synthesis of the results was performed concerning types of delivery, analgesia use, pain perception, and maternal satisfaction with the water birth experience. Conclusions: In this study, we conclude that the results regarding delivery types, labor durations, and analgesia use found in the literature, along with statistically significant maternal/fetal effects, are crucial for making recommendations regarding the use of water during labor in any of its stages if the woman desires it safely.
The Commitment to Excellence: Understanding Nurses’ Perspectives on Continuous Professional Development
Authors: Kurtović Biljana , Gulić Petra , Čukljek Snježana , Sedić Biserka , Smrekar Martina , Ledinski Fičko Sanja
Published: February 2024

Continuous Professional Development (CPD) is essential for nurses to maintain up-to-date knowledge and skills in the evolving healthcare sector. This study explores nurses’ attitudes toward CPD, its necessity, and the challenges encountered. The aim is to examine nurses’ perspectives on CPD, focusing on their participation and motivation, in relation to their workplace, workplace function, and form of work. A cross-sectional study design was employed with 151 nurses from University Hospital Centre Split, Croatia. Data were collected using the “Professional Development of Nurses questionnaire (Q-PDN)” and analyzed using descriptive statistical methods, the Kolmogorov–Smirnov test, Pearson’s correlation, ANOVA test, and t-test. The average level of participation in CPD activities was 4.27 (±0.63), indicating a positive inclination towards CPD. The study identified a statistically significant difference in activities related to CPD (t = 2.12; p = 0.036) among employees of surgical and intensive care units compared to other departments, where a higher level of engagement was present among employees of other departments. Notably, nurses without managerial roles showed 0.16 points higher participation in CPD activities compared to their managerial counterparts, though this was not statistically significant (t = 0.92; p = 0.357). Nurses in managerial roles valued CPD for professional development more highly, with a significant difference (t = 2.77; p = 0.006). Full-time nurses demonstrated a higher perception of the importance of personal professional development compared to part-time nurses, with a significant difference (F = 2.88; p = 0.038). The study reveals a strong commitment to CPD among nurses, with variations based on workplace roles and schedules. It underscores the need for role-specific and adaptable CPD programs to meet diverse needs and enhance professional competence in the nursing workforce.
The Impact of Three Key Paradigm Shifts on Disability, Inclusion, and Autism in Higher Education in England: An Integrative Review
Authors: Tang Eunice S. Y. , Griffiths Austin , Welch Graham F.
Published: February 2024

In the past two decades, students have been more willing to disclose their disability status when entering higher education (HE) in the United Kingdom (UK). Concurrently, higher education institutions (HEIs) have adopted disability policies and service teams for enhancing equality, diversity, and inclusion in the UK. The purpose of this integrative review is to understand the basis of these trends. The article suggests that there have been three major key paradigm shifts that underpin this cultural change. (1) There was a paradigm shift in terms of changing the dominant models for conceptualising disability from a medical model of disability to a social model of disability and to an affirmative model of disability, together with a debate and policy development demonstrating a concern for greater social inclusion and exclusion; (2) with a massive increase in students entering HE and the rising importance of league tables and ranking systems, universities experienced a paradigm shift from teacher-centred learning (TCL) to inclusive student-centred learning and teaching (SCLT); and (3) the increase in autism disclosure in HE signifies a shift in a conceptualisation of autism from a disorder to a disability and an example of neurodiversity.
The Effect and Potential Mechanism of Fulvic Acid on Flavonoids in Lemon Leaves
Authors: Ren Youdi , Yang Fan , Dai Weifeng , Yuan Cheng , Qin Yi , Li Jinxue , Zhang Mi
Published: February 2024

Citrus limon (L.) Burm. f. is a horticultural crop known for its abundance of valuable secondary metabolites, including flavonoids, which are found in its fruits and leaves. Our previous research has shown that treating C. limon with fulvic acid (FA) can enhance the levels of vitamin C, total acid, total sugar, total flavonoids, and phenols in its fruits as well as the volatiles and total flavonoids in its leaves. In this current study, we established a method to analyze eight specific flavonoids in lemon leaves and evaluated the impact of irrigation with FA on the content of these flavonoids over a six-month period using HPLC-DAD/MS analysis. Moreover, we investigated the potential mechanisms of FA through ELISA and q-PCR methods. The results indicated that FA increased the contents of four flavonoids, namely, isoorientin, eriocitrin, vitexin, and rutin, and promoted the activity and gene expression of phenylalanineammonialyase (PAL), 4-coumaric acid coenzyme A ligase (4CL), chalcone synthase (CHS), flavonoid 3′-hydroxylase (F3′H), and flavonol synthase (FLS). Furthermore, the relationship between flavonoid content and the activities of biosynthetic enzymes was analyzed using orthogonal partial least squares discriminant analysis (OPLS-DA), which revealed a positive correlation between seven flavonoid levels and the activity of five biosynthetic enzymes under FA treatment.
Presenilin: A Multi-Functional Molecule in the Pathogenesis of Alzheimer’s Disease and Other Neurodegenerative Diseases
Authors: Sun Yang , Islam Sadequl , Michikawa Makoto , Zou Kun
Published: February 2024

Presenilin, a transmembrane protein primarily known for its role in Alzheimer’s disease (AD) as part of the γ-secretase complex, has garnered increased attention due to its multifaceted functions in various cellular processes. Recent investigations have unveiled a plethora of functions beyond its amyloidogenic role. This review aims to provide a comprehensive overview of presenilin’s diverse roles in AD and other neurodegenerative disorders. It includes a summary of well-known substrates of presenilin, such as its involvement in amyloid precursor protein (APP) processing and Notch signaling, along with other functions. Additionally, it highlights newly discovered functions, such as trafficking function, regulation of ferritin expression, apolipoprotein E (ApoE) secretion, the interaction of ApoE and presenilin, and the Aβ42-to-Aβ40-converting activity of ACE. This updated perspective underscores the evolving landscape of presenilin research, emphasizing its broader impact beyond established pathways. The incorporation of these novel findings accentuates the dynamic nature of presenilin’s involvement in cellular processes, further advancing our comprehension of its multifaceted roles in neurodegenerative disorders. By synthesizing evidence from a range of studies, this review sheds light on the intricate web of presenilin functions and their implications in health and disease.
Comparative Solution Equilibrium Studies on Anticancer Estradiol-Based Conjugates and Their Copper Complexes
Authors: Enyedy Éva A. , Giricz Anett , Petrasheuskaya Tatsiana V. , Mészáros János P. , May Nóra V. , Spengler Gabriella , Kovács Ferenc , Molnár Barnabás , Frank Éva
Published: February 2024

Steroids are often considered valuable molecular tools for the development of anticancer agents with improved pharmacological properties. Conjugation of metal chelating moieties with a lipophilic sterane backbone is a viable option to obtain novel anticancer compounds. In this work, two estradiol-based hybrid molecules (PMA-E2 and DMA-E2) with an (N,N,O) binding motif and their Cu(II) complexes were developed. The lipophilicity, solubility, and acid-base properties of the novel ligands were determined by the combined use of UV-visible spectrophotometry, pH-potentiometry, and 1H NMR spectroscopy. The solution speciation and redox activity of the Cu(II) complexes were also investigated by means of UV-visible and electron paramagnetic resonance spectroscopy. Two structurally analogous ligands (PMAP and DMAP) were also included in the studies for better interpretation of the solution chemical data obtained. Three pKa values were determined for all ligands, revealing the order of the deprotonation steps: pyridinium-NH+ or NH(CH3)2+, secondary NH2+, and OH. The dimethylamine derivatives (DMA-E2, DMAP) are found in their H2L+ forms in solution at pH 7.4, whereas the fraction of the neutral HL species is significant (34–37%) in the case of the pyridine nitrogen-containing derivatives (PMA-E2, PMAP). Both estradiol derivatives were moderately cytotoxic in human breast (MCF-7) and colon adenocarcinoma (Colo-205) cells (IC50 = 30–63 μM). They form highly stable complexes with Cu(II) ions capable of oxidizing ascorbate and glutathione. These Cu(II) complexes are somewhat more cytotoxic (IC50 = 15–45 μM) than their corresponding ligands and show a better selectivity profile.
Aortic Valve Embryology, Mechanobiology, and Second Messenger Pathways: Implications for Clinical Practice
Authors: Notenboom Maximiliaan L. , Van Hoof Lucas , Schuermans Art , Takkenberg Johanna J. M. , Rega Filip R. , Taverne Yannick J. H. J.
Published: February 2024

During the Renaissance, Leonardo Da Vinci was the first person to successfully detail the anatomy of the aortic root and its adjacent structures. Ever since, novel insights into morphology, function, and their interplay have accumulated, resulting in advanced knowledge on the complex functional characteristics of the aortic valve (AV) and root. This has shifted our vision from the AV as being a static structure towards that of a dynamic interconnected apparatus within the aortic root as a functional unit, exhibiting a complex interplay with adjacent structures via both humoral and mechanical stimuli. This paradigm shift has stimulated surgical treatment strategies of valvular disease that seek to recapitulate healthy AV function, whereby AV disease can no longer be seen as an isolated morphological pathology which needs to be replaced. As prostheses still cannot reproduce the complexity of human nature, treatment of diseased AVs, whether stenotic or insufficient, has tremendously evolved, with a similar shift towards treatments options that are more hemodynamically centered, such as the Ross procedure and valve-conserving surgery. Native AV and root components allow for an efficient Venturi effect over the valve to allow for optimal opening during the cardiac cycle, while also alleviating the left ventricle. Next to that, several receptors are present on native AV leaflets, enabling messenger pathways based on their interaction with blood and other shear-stress-related stimuli. Many of these physiological and hemodynamical processes are under-acknowledged but may hold important clues for innovative treatment strategies, or as potential novel targets for therapeutic agents that halt or reverse the process of valve degeneration. A structured overview of these pathways and their implications for cardiothoracic surgeons and cardiologists is lacking. As such, we provide an overview on embryology, hemodynamics, and messenger pathways of the healthy and diseased AV and its implications for clinical practice, by relating this knowledge to current treatment alternatives and clinical decision making.
Perceived Parental Style Is Better in Adults with Congenital Heart Disease than Healthy Controls—But There Is Work Left to Do in Specific Subgroups
Authors: Brudy Leon , Hock Julia , Willinger Laura , Oberhoffer-Fritz Renate , Hager Alfred , Ewert Peter , Müller Jan
Published: February 2024

Objective: To compare perceived parental style in a large cohort of adults with congenital heart disease (ACHD) to healthy reference (RCs). Furthermore, factors associated with perceived parental style were determined in ACHD. Patients and Methods: From September 2016 to April 2019, 912 ACHD (34.9 ± 10.4 years, 45% female) and 175 RCs (35.8 ± 12.2 years, 53% female) completed the Measure of Parental Style (MOPS) questionnaire. Results: After adjusting for age and sex, ACHD recalled the parental style of both their parents to be significantly less indifferent (mother: ACHD: 1.2 ± 0.01 vs. RC: 1.3 ± 0.03, p < 0.001; father: ACHD: 1.3 ± 0.02 vs. RC: 1.7 ± 0.05, p < 0.001), overcontrolling (mother: ACHD: 1.6 ± 0.63 vs. RC: 1.9 ± 0.62, p < 0.001; father: ACHD: 1.4 ± 0.52 vs. RC: 1.5 ± 0.50, p < 0.001), and abusive (mother: ACHD: 1.2 ± 0.47 vs. RC: 1.4 ± 0.46, p < 0.001; father: ACHD: 1.3 ± 0.59 vs. RC: 1.5 ± 0.57, p < 0.001) than healthy controls did. In ACHD, female sex (β = 0.068, p = 0.017), higher age (β = 0.005, p = 0.003), Ebstein anomaly (β = 0.170, p = 0.005), and cyanotic CHD (β = 0.336, p = 0.004) contribute to perceiving the parental style of at least one of the parents negatively. Conclusions: While ACHD appear to recall the parental style to be less negative, subgroup analysis revealed specific patients at risk. These findings point to the need for interventions in specific subgroups susceptible to psychological distress.
Sim-to-Real Dataset of Industrial Metal Objects
Authors: De Roovere Peter , Moonen Steven , Michiels Nick , wyffels Francis
Published: February 2024

We present a diverse dataset of industrial metal objects with unique characteristics such as symmetry, texturelessness, and high reflectiveness. These features introduce challenging conditions that are not captured in existing datasets. Our dataset comprises both real-world and synthetic multi-view RGB images with 6D object pose labels. Real-world data were obtained by recording multi-view images of scenes with varying object shapes, materials, carriers, compositions, and lighting conditions. This resulted in over 30,000 real-world images. We introduce a new public tool that enables the quick annotation of 6D object pose labels in multi-view images. This tool was used to provide 6D object pose labels for all real-world images. Synthetic data were generated by carefully simulating real-world conditions and varying them in a controlled and realistic way. This resulted in over 500,000 synthetic images. The close correspondence between synthetic and real-world data and controlled variations will facilitate sim-to-real research. Our focus on industrial conditions and objects will facilitate research on computer vision tasks, such as 6D object pose estimation, which are relevant for many industrial applications, such as machine tending. The dataset and accompanying resources are available on the project website.
A Detailed Protocol for Constructing a Human Single-Chain Variable Fragment (scFv) Library and Downstream Screening via Phage Display
Authors: Liu Ziyi , Kim Dokyun , Kang Seokmin , Jung Jae U.
Published: February 2024

The development of monoclonal antibodies (mAbs) represents a significant milestone in both basic research and clinical applications due to their target specificity and versatility in therapeutic and diagnostic applications. The innovative strategy of mAb screening, utilizing phage display, facilitates the in vitro screening of antibodies with high affinity to target antigens. The single-chain variable fragment (scFv) is a subset of mAb derivatives, known for its high binding affinity and smaller size—just one-third of that of human IgG. This report outlines a detailed and comprehensive procedure for constructing a scFv phagemid library derived from human patients, followed by screening via phage display affinity selection. The protocol utilizes 348 primer combinations spanning the entire human antibody repertoire to minimize sequence bias and maintain library diversity during polymerase chain reaction (PCR) for scFv generation, resulting in a library size greater than 1 × 108. Furthermore, we describe a high-throughput phage display screening protocol using enzyme-linked immunosorbent assay (ELISA) to evaluate more than 1200 scFv candidates. The generation of a highly diverse scFv library, coupled with the implementation of a phage display screening methodology, is expected to provide a valuable resource for researchers in pursuit of scFvs with high affinity for target antigens, thus advancing both research and clinical endeavors.
Organic Nitrogen Forms in Soils Treated with Cattle Manure
Authors: Adame Cassia Rita , Carlos Roberta Souto , Braos Lucas Boscov , Ferreira Manoel Evaristo , da Cruz Mara Cristina Pessôa
Published: February 2024

The availability of nitrogen in the soil is influenced by several factors associated with the forms and characteristics of organic nitrogen present in it, as well as by any property that impacts its microbiological and biochemical activity. The objective of the present work was to evaluate the combined effect of manure fertilization, soil type, and incubation time on soil N forms and availability. The experiment was conducted in a factorial scheme (2 soils × with (20 Mg ha−1) or without manure × 4 incubation times (15, 45, 90, and 180 days)). The levels of organic matter and fractions of organic N and mineral N were evaluated. Fertilization increased the organic matter and mineral N content in both soils, but the effect of fertilization on the organic N fractions was different depending on the type of soil. In the clayey soil, there was an accumulation of nitrogen in the unidentified hydrolysable fraction, whereas, in the sandy soil, the greatest increase occurred in the hydrolysable ammonium fraction. The application of manure generated different effects on the dynamics of N forms, depending on the type of soil. It is important to understand the impacts of different soil properties on the forms of nitrogen.
Synthesis and Butyllithium-Induced Cyclisation of 2-Benzyloxyphenylphosphonamidates Giving 2,3-Dihydrobenzo[d][1,3]oxaphospholes
Authors: Aitken R. Alan , Ait Moulay Khadija , Cordes David B. , Inwood Ryan A. , Jamieson Fraser G. , Nelson Alexander J. B. , McKay Aidan P.
Published: February 2024

A series of fourteen O-ethyl-N-butylphenylphosphonamidates with benzyl ether substituents at the ortho position was prepared and fully characterised. Upon treatment with n-butyllithium in THF at RT, they underwent cyclisation in eight cases to give the novel 2,3-dihydrobenzo[d][1,3]oxaphospholes in moderate to low yield as a single diastereomer, for which the relative configuration was determined by X-ray diffraction in one case.
Assessment of Physicians’ Practice in Implementing Antibiotic Stewardship Program in Najran City, Saudi Arabia: A Cross-Sectional Study
Authors: Alqahtani Nasser Saeed , Bilal Maha Mohammed , Al Margan Albatoul M. , Albaghrah Fatimah Ahmad , Al Sharyan Anwar Mana , Alyami Aljawharh Salem M.
Published: February 2024

Introduction: One of the main causes of illness, mortality, and rising medical costs is antimicrobial resistance, which is a global healthcare concern. Objectives: This study explores the practice of physicians toward the effective implementation of Antibiotic Stewardship Programs (ASPs) in Najran city, Saudi Arabia. Methodology: This cross-sectional study was conducted among physicians working at primary care setting in Najran city, Saudi Arabia, between May and August 2023. A self-administered questionnaire was distributed among the physicians composed of three parts: socio-demographic data, a questionnaire about physicians’ practice in the efficacy of ASP, and a questionnaire about physicians’ practice regarding prescribing antibiotics. Results: Of the 128 physicians who participated in the study, 60.2% were males, and 43.8% were aged between 36 and 45 years. Among the practices in implementing the ASP effectively, controlling the source of infection domain received the highest score (mean score: 4.83). Every practice domain mean score was greater than 3, indicating that study participants possessed a moderate level of ASP practice and implementation skills. The overall mean practice score in the effective implementation of ASP was 154.9 ± 25.5 out of 185 points, with good, moderate, and poor practices constituting 67.2%, 28.1%, and 4.7%, respectively. Conclusions: The physicians showed a moderate level of practice for the effective implementation of ASPs in Najran city. The factors significantly associated with increased practice score include older age, male gender, Saudi nationality, handling five or fewer infection cases daily, and infection-initiated antibiotic prescribing treatment managed per day. These findings suggest the need for targeted interventions and educational programs to enhance physicians’ adherence to ASP guidelines and promote appropriate antibiotic prescribing practices, ultimately contributing to global efforts in combating antimicrobial resistance and improving patient outcomes.