bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Antioxidant and Antiproliferative Properties of Cornus mas L. Fruit Juice
Authors: Tiptiri-Kourpeti Angeliki , Fitsiou Eleni , Spyridopoulou Katerina , Vasileiadis Stavros , Iliopoulos Christos , Galanis Alex , Vekiari Stavroula , Pappa Aglaia , Chlichlia Katerina
Published: September 2019

Cornus mas L. (Cornelian cherry) is a flowering plant indigenous to Europe and parts of Asia, mostly studied for the antimicrobial activity of its juice. In this report, we investigated the composition and the in vitro antioxidant capacity of Cornus mas L. fruit juice from Greece, as well as its antiproliferative properties in vitro and in vivo. The fruits showed a high content of citric, malic, and succinic acid, in contrast to their juice, which had a low concentration of organic acids. The juice demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and modest antiproliferative potential against four human cancer cells lines and one murine: mammary adenocarcinoma MCF-7, hepatocellular carcinoma HepG2 and colon adenocarcinomas Caco2, HT-29, as well as murine colon carcinoma CT26. Cell viability was reduced by 40–50% following incubation of the cells with the highest concentration of the juice. Although Cornelian cherry juice exhibited in vitro growth inhibitory effects against colon carcinoma cells, no tumor growth inhibition was observed in an in vivo experimental colon carcinoma model in mice following prophylactic oral administration of a daily dose of 100 μL juice for a period of 10 days. Thus, our findings raise interesting questions for further research on Cornus mas L. fruit juice, and in parallel, the strong antioxidant potential implies that the plant could be further explored and exploited for its protective effect against oxidative damage.
Peracetic Acid Sterilization Induces Divergent Biological Response in Polymeric Tissue Engineering Scaffolds
Authors: Yoganarasimha Suyog , Best Al , Madurantakam Parthasarathy A.
Published: September 2019

Synthetic polymers offer control over composition, architecture, mechanical properties and degradation kinetics. Predictable sterilization of synthetic polymeric scaffolds made from low temperature melting polymers, remains a challenge to clinical translation. We previously demonstrated successful room temperature sterilization of electrospun polycaprolactone scaffolds (ePCL) using peracetic acid (PA). The current paper investigates the effects of PA sterilization on two different scaffolds types—ePCL and commercially available porous polystyrene (Alvetex®) scaffolds using mouse calvarial osteoblasts cell line (MC3T3) and Live-Dead Assay. We report cytotoxicity in PA-treated ePCL scaffolds (PA-ePCL), while control scaffolds strongly supported cell survival. Treatment of PA-ePCL scaffolds with known methods of PA residual elimination (sodium thiosulfate, catalase, washing and aeration) had minimal effect on MC3T3 survival. However, incubation in 80% ethanol for 30 min successfully eliminated the toxic PA residuals and restored scaffold cytocompatibility. On the other hand, PA treatment of Alvetex® scaffolds induced diametrically opposite effects: cell survival and proliferation was enhanced after PA exposure and these responses were reversed following ethanol wash. These results suggest that PA treatment can induce different biological effects based on polymer chemistry and scaffold architecture and presents interesting opportunities to modulate biological properties of tissue engineering scaffolds.
Investigation on the Performance Improvement of Polyacrylonitrile-Derived Flexible Electrospun Carbon Nanofiber Mats
Authors: He Xin , Lan Qiuming , Zhao Sirou , Liu Junyan , Zhang Chi , Chen Bohua , Chen Mei , Song Mingxia
Published: September 2019

Polyacrylonitrile (PAN)-derived carbon nanofiber mats were fabricated using electrospinning and further carbonization. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and electrochemical characterization were used to investigate the effects of precursor concentration, thermal stabilization and carbonization temperature, addition of multi-walled carbon nanotubes (MWCNTs), activation of nitric acid and sulfuric acid on the morphologies, conductivity, flexibility and electrochemical properties of the fabricated carbon nanofiber mats. The results reveal that the carbon nanofiber mats with uniform fiber diameter of 200 nm and sheet resistance of 154 Ω/sq could be achieved with a PAN mass fraction of 12 wt% and a thermal stabilization and carbonization temperature of 270 °C and 900 °C, respectively. Due to the good conductivity and high strength of the MWCNTs, the sheet resistance of the carbon nanofiber mats decreases to around 60 Ω/sq by adding MWCNTs to precursor, and the mats exhibit excellent bend and fold flexibility. The electrochemical performance of the co-spun carbon nanofiber mats could be further improved by the activation treatment of acids, and the maximum specific capacitance of the carbon mat reaches 113.5 F/g at a current density of 0.1 mA/cm2 in the case of 1:3 HNO3:H2SO4. The investigation provides a reference for improving the performance of spun carbon nanofiber mats, which can be used as the electrodes or current collectors to further load other active materials in the applications of energy storage devices.
Effect of Venturi Structures on the Cleaning Performance of a Pulse Jet Baghouse
Authors: Liu Xingcheng , Shen Henggen
Published: September 2019

In order to study the effect of venturi structures on the cleaning performance of a pulse jet baghouse, several cleaning tests are carried out applying the pulse jet test bench. Experiments are performed using different filter bags with venturi tubes of varying throat diameters and lengths. Peak pressure and peak pressure arrival time are selected to evaluate the cleaning efficiency under different conditions. On this basis, a series of comparative experiments are conducted between a typical venturi-type and a new venturi tube design on a semi-industrial pulse jet baghouse. The effects of the new venturi-type on baghouse cleaning performance and dust emission are studied. The results show that pulsing air pressure has a significant effect on bag-cleaning performance; the larger the throat diameter and the shorter the venturi length, the higher the peak pressure and the shorter the peak pressure arrival time. This improves the cleaning efficiency of the filter bags. The new venturi design changes the distribution of the cleaning pressure in the vicinity of the bag mouth, effectively extending the cleaning cycle and reducing the energy consumption as well as reducing the dust emission concentration of the baghouse.
PPDC: A Privacy-Preserving Distinct Counting Scheme for Mobile Sensing
Authors: Yang Xiaochen , Xu Ming , Fu Shaojing , Luo Yuchuan
Published: September 2019

Mobile sensing mines group information through sensing and aggregating users’ data. Among major mobile sensing applications, the distinct counting problem aiming to find the number of distinct elements in a data stream with repeated elements, is extremely important for avoiding waste of resources. Besides, the privacy protection of users is also a critical issue for aggregation security. However, it is a challenge to meet these two requirements simultaneously since normal privacy-preserving methods would have negative influence on the accuracy and efficiency of distinct counting. In this paper, we propose a Privacy-Preserving Distinct Counting scheme (PPDC) for mobile sensing. Through integrating the basic idea of homomorphic encryption into Flajolet-Martin (FM) sketch, PPDC allows an aggregator to conduct distinct counting over large-scale datasets without disrupting privacy of users. Moreover, PPDC supports various forms of sensing data, including camera images, location data, etc. PPDC expands each bit of the hashing values of users’ original data, FM sketch is thus enhanced for encryption to protect users’ privacy. We prove the security of PPDC under known-plaintext model. The theoretic and experimental results show that PPDC achieves high counting accuracy and practical efficiency with scalability over large-scale data sets.
Scale-Adaptive Simulation of Unsteady Cavitation Around a Naca66 Hydrofoil
Authors: Hidalgo Víctor , Escaler Xavier , Valencia Esteban , Peng Xiaoxing , Erazo José , Puga Diana , Luo Xianwu
Published: September 2019

The present paper focuses on the numerical simulation of unsteady cavitation around a NACA66 hydrofoil to improve the understanding of the cavitation effects on hydraulic machinery. For this aim, the Zwart–Gerber–Belamri cavitation model was updated and uploaded as a library file for OpenFOAM’s solvers using C++ language. Furthermore, the hybrid Reynold average Navier–Stokes (RANS)–large eddy simulation (LES) model k - ω SST scale adaptive simulation (SAS) was implemented as a turbulence model for the present study of scale adaptive simulation. For validation, numerical results were compared with experimental results obtained by Leroux at the Naval Academy Research Institute in France. In order to highlight the benefits in terms of computational consumption and reproduction of the phenomenon the k - ω SST SAS model was compared against implicit large eddy simulation (ILES). Results show that the cavitation evolution including the maximum vapor length, the detachment and the oscillation frequency were reproduced satisfactorily using k - ω SST SAS. Moreover, k - ω SST SAS results predicted a lower total vapor volume on time than ILES, which is related to observed pulses of pressure coefficient, C p , and those match fairly well with the experimental results. To summarize, the k - ω SST SAS model predicts with good accuracy unsteady cavitation behavior around hydrofoils and shows improved versatility over the ILES approach.
An Analysis of the Short Utterance Problem for Speaker Characterization
Authors: Viñals Ignacio , Ortega Alfonso , Miguel Antonio , Lleida Eduardo
Published: September 2019

Speaker characterization has always been conditioned by the length of the evaluated utterances. Despite performing well with large amounts of audio, significant degradations in performance are obtained when short utterances are considered. In this work we present an analysis of the short utterance problem providing an alternative point of view. From our perspective the performance in the evaluation of short utterances is highly influenced by the phonetic similarity between enrollment and test utterances. Both enrollment and test should contain similar phonemes to properly discriminate, being degraded otherwise. In this study we also interpret short utterances as incomplete long utterances where some acoustic units are either unbalanced or just missing. These missing units are responsible for the speaker representations to be unreliable. These unreliable representations are biased with respect to the reference counterparts, obtained from long utterances. These undesired shifts increase the intra-speaker variability, causing a significant loss of performance. According to our experiments, short utterances (3–60 s) can perform as accurate as if long utterances were involved by just reassuring the phonetic distributions. This analysis is determined by the current embedding extraction approach, based on the accumulation of local short-time information. Thus it is applicable to most of the state-of-the-art embeddings, including traditional i-vectors and Deep Neural Network (DNN) xvectors.
Neural Machine Reading Comprehension: Methods and Trends
Authors: Liu Shanshan , Zhang Xin , Zhang Sheng , Wang Hui , Zhang Weiming
Published: September 2019

Machine reading comprehension (MRC), which requires a machine to answer questions based on a given context, has attracted increasing attention with the incorporation of various deep-learning techniques over the past few years. Although research on MRC based on deep learning is flourishing, there remains a lack of a comprehensive survey summarizing existing approaches and recent trends, which motivated the work presented in this article. Specifically, we give a thorough review of this research field, covering different aspects including (1) typical MRC tasks: their definitions, differences, and representative datasets; (2) the general architecture of neural MRC: the main modules and prevalent approaches to each; and (3) new trends: some emerging areas in neural MRC as well as the corresponding challenges. Finally, considering what has been achieved so far, the survey also envisages what the future may hold by discussing the open issues left to be addressed.
An Innovative Green Process for the Stabilization and Valorization of Organic Fraction of Municipal Solid Waste (OFMSW): Optimization of the Curing Process II Part
Authors: Esposito Corcione Carola , Ferrari Francesca , Striani Raffaella , Visconti Paolo , Greco Antonio
Published: September 2019

This work is focused on the optimization of an innovative and cheap process for the valorization of the organic fraction of municipal solid waste (OFMSW), through its transformation into an odorless and environmentally compatible material to be employed for building applications or as a thermal and acoustic insulator. The process starts with the grinding of OFMSW, followed by its sterilization in order to obtain a complete removal of the bacterial activity. Afterwards, the incorporation in a catalysed thermosetting matrix is carried out by mixing the OFMSW to a water soluble urea formaldehyde based resin (UF), characterized by a formaldehyde content lower than 1% wt. The OFMSW/UF blends were firstly analysed by the dynamic rheological analyses, as a function of the content of a proper catalyst, that is able to decrease the curing temperature and time. Rheological analyses results allowed the selection of times and temperatures necessary for the polymerization (T = 60 °C, t = 1 h). The effect of the presence of different additives on both the cure process and the mechanical properties of the cured samples was finally analysed, evidencing that the OFMSW/UF composites possess improved mechanical properties in comparison to that of the neat resin.
A Methodology for Verifying Cloud Forecasts with VIIRS Imagery and Derived Cloud Products—A WRF Case Study
Authors: Hutchison Keith D. , Iisager Barbara D. , Dipu Sudhakar , Jiang Xiaoyan , Quaas Johannes , Markwardt Randy
Published: September 2019

A methodology is presented to evaluate the accuracy of cloud cover fraction (CCf) forecasts generated by numerical weather prediction (NWP) and climate models. It is demonstrated with a case study consisting of simulations from the Weather Research and Forecasting (WRF) model. In this study, since the WRF CCf forecasts were initialized with reanalysis fields from the North American Mesoscale (NAM) Forecast System, the characteristics of the NAM CCf products were also evaluated. The procedures relied extensively upon manually-generated, binary cloud masks created from VIIRS (Visible Infrared Imager Radiometry Suite) imagery, which were subsequently converted into CCf truth at the resolution of the NAM and WRF gridded data. The initial results from the case study revealed biases toward under-clouding in the NAM CCf analyses and biases toward over-clouding in the WRF CCf products. These biases were evident in images created from the gridded NWP products when compared to VIIRS imagery and CCf truth data. Thus, additional simulations were completed to help assess the internal procedures used in the WRF model to translate moisture forecast fields into layered CCf products. Two additional sets of WRF CCf 24 h forecasts were generated for the region of interest using WRF restart files. One restart file was updated with CCf truth data and another was not changed. Over-clouded areas in the updated WRF restart file that were reduced with an update of the CCf truth data became over-clouded again in the WRF 24 h forecast, and were nearly identical to those from the unchanged restart file. It was concluded that the conversion of WRF forecast fields into layers of CCf products deserves closer examination in a future study.
Differential Sympathetic Activation of Adipose Tissues by Brain-Derived Neurotrophic Factor
Authors: Zhu Qi , Liu Xian , Glazier Bradley J. , Krolick Kristen N. , Yang Shangyuwen , He Jingyan , Lo Chunmin C. , Shi Haifei
Published: September 2019

Centrally administered brain-derived neurotrophic factor (BDNF) decreases body adiposity beyond what can be accounted for by decreased food intake, implying enhanced lipid metabolism by BDNF. Consistent with this notion, intracerebroventricular (icv) injection of BDNF in rats increased the expression of lipolytic enzymes in white adipose tissues (WAT) and increased circulating concentrations of lipolytic products without changing the levels of adrenal gland hormones. This suggests that central BDNF-induced lipid mobilization is likely due to sympathetic neural activation, rather than activation of the adrenocortical or adrenomedullary system. We hypothesized that BDNF activated sympathetic innervation of adipose tissues to regulate lipolysis. Rats with unilateral denervation of interscapular brown adipose tissue (BAT) and different WAT depots received icv injections of saline or BDNF. Both intact and denervated adipose tissues were exposed to the same circulating factors, but denervated adipose tissues did not receive neural signals. Norepinephrine (NE) turnover (NETO) of BAT and WAT was assessed as a measure of sympathetic activity. Findings revealed that central BDNF treatment induced a change in NETO in some but not all the adipose tissues tested. Specifically, greater NETO rates were found in BAT and gonadal epididymal WAT (EWAT), but not in inguinal WAT (IWAT) or retroperitoneal WAT (RWAT), of BDNF-treated rats compared to saline-treated rats. Furthermore, intact innervation was necessary for BDNF-induced NETO in BAT and EWAT. In addition, BDNF increased the expression of lipolytic enzymes in both intact and denervated EWAT and IWAT, suggesting that BDNF-induced WAT lipolysis was independent of intact innervation. To summarize, centrally administered BDNF selectively provoked sympathetic drives to BAT and EWAT that was dependent on intact innervation, while BDNF also increased lipolysis in a manner independent of intact innervation.
Wireless Direct Microampere Current in Wound Healing: Clinical and Immunohistological Data from Two Single Case Reports
Authors: Lagoumintzis George , Zagoriti Zoi , Jensen Mogens S. , Argyrakos Theodoros , Koutsojannis Constantinos , Poulas Konstantinos
Published: September 2019

Chronic pressure ulcers are hard-to-heal wounds that decrease the patient’s quality of life. Wireless Micro Current Stimulation (WMCS) is an innovative, non-invasive, similar to electrode-based electrostimulation (ES) technology, that generates and transfers ions that are negatively-charged to the injured tissue, using accessible air gases as a transfer medium. WMCS is capable of generating similar tissue potentials, as electrode-based ES, for injured tissue. Here, through immunohistochemistry, we intended to characterize the induced tissue healing biological mechanisms that occur during WMCS therapy. Two single cases of bedridden due to serious stroke white men with chronic non-healing pressure ulcers have been treated with WMCS technology. WMCS suppresses inflammatory responses by decreasing the aggregation of granulocytes, followed by stimulating myofibroblastic activity and a new formation of collagen fibers, as depicted by immunohistochemistry. As a result, WMCS provides a special adjunct or stand-alone therapy choice for chronic and non-healing injuries, similar to electrode-based ES, but with added (i.e., contactless) benefits towards its establishment as a routine clinical wound healing regime.
Prediction of the Maximum Seismic Member Force in a Superstructure of a Base-Isolated Frame Building by Using Pushover Analysis
Authors: Fujii Kenji
Published: September 2019

It is essential for the seismic design of a base-isolated building that the seismic response of the superstructure remains within the elastic range. The evaluation of the maximum seismic member force in a superstructure is thus an important issue. The present study predicts the maximum seismic member force of five- and fourteen-story reinforced concrete base-isolated frame buildings adopting pushover analysis. In the first stage of the study, the nonlinear dynamic (time-history) analysis of the base-isolated frame buildings is carried out, and the nonlinear modal responses of the first and second modes are calculated from pushover analysis results. In the second stage, a set of pushover analyses is proposed considering the combination of the first and second modal responses, and predicted maximum member forces are compared with the nonlinear time-history analysis results. Results show that the maximum member forces predicted in the proposed set of pushover analyses are satisfactorily accurate, while the results predicted considering only the first mode are inaccurate.
GLUT1 and TUBB4 in Glioblastoma Could be Efficacious Targets
Authors: Guda Maheedhara R. , Labak Collin M. , Omar Sara Ibrahim , Asuthkar Swapna , Airala Subra , Tuszynski Jack , Tsung Andrew J. , Velpula Kiran K.
Published: September 2019

Glioblastoma multiforme (GBM) is the most aggressive and deadly brain tumor, portending a median 13-month survival even following gross total resection with adjuvant chemotherapy and radiotherapy. This prognosis necessitates improved therapies for the disease. A target of interest for novel chemotherapies is the Warburg Effect, which describes the tumor’s shift away from oxidative phosphorylation towards glycolysis. Here, we elucidate GLUT1 (Glucose transporter 1) and one of its associated binding partners, TUBB4 (Tubulin 4), as potentially druggable targets in GBM. Using data mining approach, we demonstrate that GLUT1 is overexpressed as a function of tumor grade in astrocytoma’s and that its overexpression is associated with poorer prognosis. Using both mass spectrometry performed on hGBM (human glioblastoma patient specimen) and in silico modeling, we show that GLUT1 interacts with TUBB4, and more accurately demonstrates GLUT1’s binding with fasentin. Proximity ligation assay (PLA) and immunoprecipitation studies confirm GLUT1 interaction with TUBB4. Treatment of GSC33 and GSC28 cells with TUBB4 inhibitor, CR-42-24, reduces the expression of GLUT1 however, TUBB4 expression is unaltered upon fasentin treatment. Using human pluripotent stem cell antibody array, we demonstrate reduced levels of Oct3/4, Nanog, Sox2, Sox17, Snail and VEGFR2 (Vascular endothelial growth factor receptor 2) upon CR-42-24 treatment. Overall, our data confirm that silencing TUBB4 or GLUT1 reduce GSC tumorsphere formation, self-renewal and proliferation in vitro. These findings suggest GLUT1 and its binding partner TUBB4 as druggable targets that warrant further investigation in GBM.
The Multifaceted Roles of Pyroptotic Cell Death Pathways in Cancer
Authors: Wang Man , Jiang Shuai , Zhang Yinfeng , Li Peifeng , Wang Kun
Published: September 2019

Cancer is a category of diseases involving abnormal cell growth with the potential to invade other parts of the body. Chemotherapy is the most widely used first-line treatment for multiple forms of cancer. Chemotherapeutic agents act via targeting the cellular apoptotic pathway. However, cancer cells usually acquire chemoresistance, leading to poor outcomes in cancer patients. For that reason, it is imperative to discover other cell death pathways for improved cancer intervention. Pyroptosis is a new form of programmed cell death that commonly occurs upon pathogen invasion. Pyroptosis is marked by cell swelling and plasma membrane rupture, which results in the release of cytosolic contents into the extracellular space. Currently, pyroptosis is proposed to be an alternative mode of cell death in cancer treatment. Accumulating evidence shows that the key components of pyroptotic cell death pathways, including inflammasomes, gasdermins and pro-inflammatory cytokines, are involved in the initiation and progression of cancer. Interfering with pyroptotic cell death pathways may represent a promising therapeutic option for cancer management. In this review, we describe the current knowledge regarding the biological significance of pyroptotic cell death pathways in cancer pathogenesis and also discuss their potential therapeutic utility.
Binding of SU(VAR)3-9 Partially Depends on SETDB1 in the Chromosomes of Drosophila melanogaster
Authors: Maksimov Daniil A. , Koryakov Dmitry E.
Published: September 2019

H3K9 methylation is known to play a critical role in gene silencing. This modification is established and maintained by several enzymes, but relationships between them are not fully understood. In the present study, we decipher the interplay between two Drosophila H3K9-specific histone methyltransferases, SU(VAR)3-9 and SETDB1. We asked whether SETDB1 is required for targeting of SU(VAR)3-9. Using DamID-seq, we obtained SU(VAR)3-9 binding profiles for the chromosomes from larval salivary glands and germline cells from adult females, and compared profiles between the wild type and SETDB1-mutant backgrounds. Our analyses indicate that the vast majority of single copy genes in euchromatin are targeted by SU(VAR)3-9 only in the presence of SETDB1, whereas SU(VAR)3-9 binding at repeated sequences in heterochromatin is largely SETDB1-independent. Interestingly, piRNA clusters 42AB and 38C in salivary gland chromosomes bind SU(VAR)3-9 regardless of SETDB1, whereas binding to the same regions in the germline cells is SETDB1-dependent. In addition, we compared SU(VAR)3-9 profiles in female germline cells at different developmental stages (germarium cells in juvenile ovaries and mature nurse cells). It turned out that SU(VAR)3-9 binding is influenced both by the presence of SETDB1, as well as by the differentiation stage.
Efficacy of 3D Culture Priming is Maintained in Human Mesenchymal Stem Cells after Extensive Expansion of the Cells
Authors: Bartosh Thomas J. , Ylostalo Joni H.
Published: September 2019

The use of non-optimal preparations of mesenchymal stem cells (MSCs), such as extensively expanded cells, might be necessary to obtain the large numbers of cells needed for many clinical applications. We previously demonstrated that minimally expanded (early passage) MSCs can be pre-activated as spheroids to produce potentially therapeutic factors in 3D cultures. Here, we used extensively expanded (late passage) MSCs and studied their 3D-culture activation potential. MSCs were culture-expanded as 2D monolayers, and cells from various passages were activated by 3D culture in hanging drops with either fetal bovine serum (FBS)-containing media or a more clinically-applicable animal product-free (xeno-free) media. Gene expression analyses demonstrated that MSC spheroids prepared from passage 3, 5, and 7 cells were similar to each other but different from 2D MSCs. Furthermore, the expression of notable anti-inflammatory/immune-modulatory factors cyclooxygenase-2 (PTGS2), TNF alpha induced protein 6 (TNFAIP6), and stanniocalcin 1 (STC-1) were up-regulated in all spheroid preparations. This was confirmed by the detection of secreted prostaglandin E2 (PGE-2), tumor necrosis factor-stimulated gene 6 (TSG-6, and STC-1. This study demonstrated that extensively expanded MSCs can be activated in 3D culture through spheroid formation in both FBS-containing and xeno-free media. This work highlights the possibility of activating otherwise less useable MSC preparations through 3D culture generating large numbers of potentially therapeutic MSCs.
Phenotypical and Functional Polymorphism of Liver Resident Macrophages
Authors: Elchaninov Andrey V. , Fatkhudinov Timur Kh. , Vishnyakova Polina A. , Lokhonina Anastasia V. , Sukhikh Gennady T.
Published: September 2019

Liver diseases are one of the main causes of mortality. In this regard, the development of new ways of reparative processes stimulation is relevant. Macrophages play a leading role in the regulation of liver homeostasis in physiological conditions and in pathology. In this regard, the development of new liver treatment methods is impossible without taking into account this cell population. Resident macrophages of the liver, Kupffer cells, represent a unique cell population, first of all, due to their development. Most of the liver macrophages belong to the self-sustaining macrophage cell population, whose origin is not bone marrow. In addition, Kupffer cells are involved in such processes as regulation of hepatocyte proliferation and apoptosis, remodeling of the intercellular matrix, lipid metabolism, protective function, etc. Such a broad spectrum of liver macrophage functions indicates their high functional plasticity. The review summarizes recent data on the development, phenotypic and functional plasticity, and participation in the reparative processes of liver macrophages: resident macrophages (Kupffer cells) and bone marrow-derived macrophages.
Hematopoietic-Extrinsic Cues Dictate Circadian Redistribution of Mature and Immature Hematopoietic Cells in Blood and Spleen
Authors: Stenzinger Miriam , Karpova Darja , Unterrainer Christian , Harenkamp Sabine , Wiercinska Eliza , Hoerster Keven , Pfeffer Martina , Maronde Erik , Bonig Halvard
Published: September 2019

Circadian oscillations in circulating leukocyte subsets including immature hematopoietic cells have been appreciated; the origin and nature of these alterations remain elusive. Our analysis of wild-type C57BL/6 mice under constant darkness confirmed circadian fluctuations of circulating leukocytes and clonogenic cells in blood and spleen but not bone marrow. Clock gene deficient Bmal1−/− mice lacked this regulation. Cell cycle analyses in the different hematopoietic compartments excluded circadian changes in total cell numbers, rather favoring shifting hematopoietic cell redistribution as the underlying mechanism. Transplant chimeras demonstrate that circadian rhythms within the stroma mediate the oscillations independently of hematopoietic-intrinsic cues. We provide evidence of circadian CXCL12 regulation via clock genes in vitro and were able to confirm CXCL12 oscillation in bone marrow and blood in vivo. Our studies further implicate cortisol as the conveyor of circadian input to bone marrow stroma and mediator of the circadian leukocyte oscillation. In summary, we establish hematopoietic-extrinsic cues as causal for circadian redistribution of circulating mature/immature blood cells.
Health and Sport. Economic and Social Impact of Active Tourism
Authors: Araújo Vila Noelia , Fraiz Brea Jose Antonio , de Araújo Arthur Filipe
Published: September 2019

Concerns with health and wellness are currently a global trend. Consequently, people have increasingly been engaging in some form of regular sports practice in their adult lives. This is evidenced by the rise of markets that are related to the sports sector. One of those market trends is active tourism, which refers to the practice of physical activities in natural environments within a tourist destination. In this context, people travel to certain places, typically with friends or as couples, to engage in sports activities of greater or lesser intensities. This allows for them to disconnect with their daily routines and provides them all of the positive health-related outcomes that are associated with exercising. Therefore, health tourism is also an efficient tool against sedentariness. The present study explores which sports are currently the most popular amongst active tourists, which attributes they value the most when choosing a destination, and what is their expenditure pattern. To this end, a sample of 60 individuals who have engaged in some form of active tourism in Galicia is surveyed. The findings suggest that active tourists are particularly interested in dedicating their discretionary time to travelling and they have a higher daily expenditure within the destination than the average tourist.
A Voltage-Based Hierarchical Diagnosis Approach for Open-Circuit Fault of Two-Level Traction Converters
Authors: Zhang Jingrong , Peng Tao , Yang Chao , Chen Zhiwen , Tao Hongwei , Yang Chunhua
Published: September 2019

This paper proposes a voltage-based hierarchical diagnosis approach for insulated gate bipolar transistors (IGBTs) open-circuit fault of two-level AC-DC-AC traction converters. The proposed approach can diagnose the open-circuit fault in the single-phase rectifier as well as in the three-phase inverter without extra sensors. Moreover, no mandatory control signal injection is required, which ensures safe operation. In addition, the different levels of diagnostic results are flexibly determined by the presented hierarchical diagnosis architecture, which depends on the market requirements of wide use of IGBT modules. To be specific, firstly, a mixed-logic-dynamic estimation model of DC-link voltage is established. Secondly, the diagnosis characteristic function (DCF) is constructed by way of a residual characteristic analysis under normal and various open-circuit fault cases. Thirdly, the vector angular similarity function (VASF) is calculated for leg-level diagnosis and the control signals switching matching method is used to locate the fault in the device-level. Finally, the experimental results show the effectiveness of the proposed approach.
Design of Efficient Perspective Affine Motion Estimation/Compensation for Versatile Video Coding (VVC) Standard
Authors: Choi Young-Ju , Jun Dong-San , Cheong Won-Sik , Kim Byung-Gyu
Published: September 2019

The fundamental motion model of the conventional block-based motion compensation in High Efficiency Video Coding (HEVC) is a translational motion model. However, in the real world, the motion of an object exists in the form of combining many kinds of motions. In Versatile Video Coding (VVC), a block-based 4-parameter and 6-parameter affine motion compensation (AMC) is being applied. In natural videos, in the majority of cases, a rigid object moves without any regularity rather than maintains the shape or transform with a certain rate. For this reason, the AMC still has a limit to compute complex motions. Therefore, more flexible motion model is desired for new video coding tool. In this paper, we design a perspective affine motion compensation (PAMC) method which can cope with more complex motions such as shear and shape distortion. The proposed PAMC utilizes perspective and affine motion model. The perspective motion model-based method uses four control point motion vectors (CPMVs) to give degree of freedom to all four corner vertices. Besides, the proposed algorithm is integrated into the AMC structure so that the existing affine mode and the proposed perspective mode can be executed adaptively. Because the block with the perspective motion model is a rectangle without specific feature, the proposed PAMC shows effective encoding performance for the test sequence containing irregular object distortions or dynamic rapid motions in particular. Our proposed algorithm is implemented on VTM 2.0. The experimental results show that the BD-rate reduction of the proposed technique can be achieved up to 0.45% and 0.30% on Y component for random access (RA) and low delay P (LDP) configurations, respectively.
Non-Cooperative Spectrum Access Strategy Based on Impatient Behavior of Secondary Users in Cognitive Radio Networks
Authors: Zeng Zhen , Liu Meng , Wang Jin , Lan Dongping
Published: September 2019

In the cognitive radio network (CRN), secondary users (SUs) compete for limited spectrum resources, so the spectrum access process of SUs can be regarded as a non-cooperative game. With enough artificial intelligence (AI), SUs can adopt certain spectrum access strategies through their learning ability, so as to improve their own benefit. Taking into account the impatience of the SUs with the waiting time to access the spectrum and the fact that the primary users (PUs) have preemptive priority to use the licensed spectrum in the CRN, this paper proposed the repairable queueing model with balking and reneging to investigate the spectrum access. Based on the utility function from an economic perspective, the relationship between the Nash equilibrium and the socially optimal spectrum access strategy of SUs was studied through the analysis of the system model. Then a reasonable spectrum pricing scheme was proposed to maximize the social benefits. Simulation results show that the proposed access mechanism can realize the consistency of Nash equilibrium strategy and social optimal strategy to maximize the benefits of the whole cognitive system.
Modeling and Analysis of an Electromagnetic Fully Variable Valve Train with a Magnetorheological Buffer
Authors: Zhu Xiangbin , Liu Liang , Guo He , Xu Zhaoping , Hou Wenguo , Niu Lu
Published: September 2019

Electromagnetic fully variable valve train (EMVT) technology promises to improve the fuel economy and optimize the engine performance. A novel EMVT equipped with a magnetorheological buffer (EMVT with MR buffer) is proposed to suppress the valve seating impact in this paper. The magnetorheological buffer can adjust the damping characteristics of the whole system in the seating process. Valve precise motion control and better seating performance can be achieved through the coordinated control of electromagnetic linear actuator (EMLA) and MR buffer. For better analysis of system performance, establishing an accurate system dynamic model is the basis of the coordinated control system. A high-order nonlinear precise model integrating dynamics, electromagnetism, and fluid mechanic was established. Then, the Jacobi linearization model is carried out at the equilibrium seating point to build a control-oriented linearized model. The correctness and accuracy of the linearized model is verified. Experiments and simulations show that the valve precise motion can be well controlled to achieve fully variable actuation. And the valve soft landing can be completed under collaborative control.
Heat to H2: Using Waste Heat for Hydrogen Production through Reverse Electrodialysis
Authors: Krakhella Kjersti , Bock Robert , Burheim Odne , Seland Frode , Einarsrud Kristian
Published: September 2019

This work presents an integrated hydrogen production system using reverse electrodialysis (RED) and waste heat, termed Heat to H 2 . The driving potential in RED is a concentration difference over alternating anion and cation exchange membranes, where the electrode potential can be used directly for water splitting at the RED electrodes. Low-grade waste heat is used to restore the concentration difference in RED. In this study we investigate two approaches: one water removal process by evaporation and one salt removal process. Salt is precipitated in the thermally driven salt removal, thus introducing the need for a substantial change in solubility with temperature, which KNO 3 fulfils. Experimental data of ion conductivity of K + and NO 3 − in ion-exchange membranes is obtained. The ion conductivity of KNO 3 in the membranes was compared to NaCl and found to be equal in cation exchange membranes, but significantly lower in anion exchange membranes. The membrane resistance constitutes 98% of the total ohmic resistance using concentrations relevant for the precipitation process, while for the evaporation process, the membrane resistance constitutes over 70% of the total ohmic resistance at 40 ∘ C. The modelled hydrogen production per cross-section area from RED using concentrations relevant for the precipitation process is 0.014 ± 0.009 m 3 h − 1 (1.1 ± 0.7 g h − 1 ) at 40 ∘ C, while with concentrations relevant for evaporation, the hydrogen production per cross-section area was 0.034 ± 0.016 m 3 h − 1 (2.6 ± 1.3 g h − 1 ). The modelled energy needed per cubic meter of hydrogen produced is 55 ± 22 kWh (700 ± 300 kWh kg − 1 ) for the evaporation process and 8.22 ± 0.05 kWh (104.8 ± 0.6 kWh kg − 1 ) for the precipitation process. Using RED together with the precipitation process has similar energy consumption per volume hydrogen produced compared to proton exchange membrane water electrolysis and alkaline water electrolysis, where the energy input to the Heat to H 2 -process comes from low-grade waste heat.
The Effect of Mixed Wastewaters on the Biomass Production and Biochemical Content of Microalgae
Authors: Park Sanghyun , Ahn Yongtae , Park Young-Tae , Ji Min-Kyu , Choi Jaeyoung
Published: September 2019

The effect of ammonia and iron concentration in Bold Basal Medium and mixed wastewater (including pretreated piggery wastewater and acid mine drainage) on biomass production and biochemical content (lipid and ß-carotene) of microalgae (Uronema sp. KGE 3) was investigated. Addition of iron to the Bold Basal Medium enhanced the growth, lipid, and ß-carotene of Uronema sp. KGE 3. The highest dry cell weight, lipid content, and lipid productivity of KGE 3 were 0.551 g L−1, 46% and 0.249 g L−1 d−1, respectively, at 15 mg L−1 of Fe. The highest ß-carotene was obtained at 30 mg L−1 of Fe. The biomass production of KGE 3 was ranged between 0.18 to 0.37 g L−1. The microalgal growth was significantly improved by addition of acid mine drainage to pretreated piggery wastewater by membrane. The highest dry cell weight of 0.51 g L−1 was obtained at 1:9 of pretreated piggery wastewater by membrane and acid mine drainage for KGE 3. The removal efficiencies of total nitrogen and total phosphate was ranged from 20 to 100%. The highest lipid and ß-carotene content was found to be 1:9. Application of this system to wastewater treatment plant could provide cost effective technology for the microalgae-based industries and biofuel production field, and also provide the recycling way for pretreated piggery wastewater and acid mine drainage.
Wetting Behavior of Alder (Alnus cordata (Loisel) Duby) Wood Surface: Effect of Thermo-Treatment and Alkyl Ketene Dimer (AKD)
Authors: Lovaglio Teresa , Gindl-Altmutter Wolfgang , Meints Tillmann , Moretti Nicola , Todaro Luigi
Published: September 2019

The main purpose of this study was to investigate the hydrophobic effect and chemical changes induced by thermo-treatment and alkyl ketene dimer (AKD) on the surface properties of Alder (Alnus cordata (Loisel) Duby) wood before and after an artificial weathering test. Thermal treatment was conducted at a temperature of 200 °C for 4 h in a thermo-vacuum cylinder. Then, the paper sizing agent, AKD at different concentrations of a solution of 0.1%, 0.5% and 10% was used as a potential hydrophobizing reagent for untreated and thermally treated alder wood surfaces. The contact angle measurement, ATR-FTIR analysis and colour variation were carried out for the samples. The preliminary results revealed that the contact angle values of the wood materials increased with thermal modification. However, the influence of the thermal treatment on hydrophobicity was small when compared to the substantial effect of the AKD application in this respect, and also after the artificial weathering test. The FTIR analysis supported the hypothesis that AKD could make bonds chemically stable even when using a small concentration of AKD. The findings acquired in this work provide important information for future research and the utilization of the AKD on lesser-used wood species.
Effects of Phosphate-Solubilizing Bacteria and N2-fixing Bacteria on Nutrient Uptake, Plant Growth, and Bioactive Compound Accumulation in Cyclocarya paliurus (Batal.) Iljinskaja
Authors: Wang Zhikang , Chen Ziyun , Xu Ziheng , Fu Xiangxiang
Published: September 2019

Research Highlights: We firstly interpreted nutritional mechanisms involved in growth regulation and phytochemical accumulation in Cyclocarya paliurus (Batal.) Iljinskaja under three inoculant types, and selected bacterial inoculations for multiple purposes of C. paliurus plantation. Co-inoculation with phosphate-solubilizing bacteria (PSB) and N2-fixing bacteria (NFB) performed better in growth promotion and nutrient uptake than single bacterial inoculation. Background and Objectives: C. paliurus is a well-known medicinal plant as it accumulates bioactive compounds (BC) such as flavonoids, triterpenoids, and polysaccharides, in its leaves. However, the effects of plant growth-promoting rhizobacteria (PGPR) on the growth and BC yields in C. paliurus are not known. To fill this gap, the effects of different inoculants should be examined. Materials and Methods: A pot experiment was conducted and two-year-old C. paliurus seedlings were inoculated with three inoculant types (PSB, NFB, PSB + NFB). After four rounds of inoculation, the growth characteristics and concentrations of flavonoids, triterpenoids, and polysaccharides, as well as the nutrients in soil and leaves, were measured. Results: The inoculations resulted in the elevation of soil available nutrients, with improvements in plant growth, BC yield, and N and P uptake in leaves. However, the changes in BC yields were mainly a result of elevated leaf biomass rather than BC concentrations, and leaf biomass was regulated by C:N:P stoichiometry. Co-inoculation with PSB and NFB was applicable for leaf production, while inocula related to NFB resulted in higher BC yields than PSB and control. Conclusions: Our results implied that bacterial inoculants improved plant growth and BC yield by altering the nutrients in soil and leaves, while three inoculant types showed a different pattern in which co-inoculation with four strains presented a greater performance than single bacterial addition.
Modeling the Potential Global Distribution of Phenacoccus madeirensis Green under Various Climate Change Scenarios
Authors: Wei Jiufeng , Li Xiaozhou , Lu Yunyun , Zhao Ling , Zhang Hufang , Zhao Qing
Published: September 2019

The Madeira mealybug, Phenacoccus madeirensis Green, is a serious invasive pest that does significant damage to more than 120 genera of host plants from 51 families in more than 81 countries. However, the potential distribution range of this pest is unclear, which could hamper control and eradication efforts. In the current study, MaxEnt models were developed to forecast the current and future distribution of the Madeira mealybug around the world. Moreover, the future potential distribution of this invasive species was projected for the 2050s and 2070s under three different climate change scenarios (HADGEM2-AO, GFDL-CM3, and MIROC5) and two representative concentration pathways (RCP-2.6 and RCP-8.5). The final model indicates that the Madeira mealybug has a highly suitable range for the continents of Asia, Europe, and Africa, as well as South America and North America, where this species has already been recorded. Potential expansions or reductions in distribution were also simulated under different future climatic conditions. Our study also suggested that the mean temperature of the driest quarter (Bio9) was the most important factor and explained 46.9% of the distribution model. The distribution model from the current and future predictions can enhance the strategic planning of agricultural and forestry organization by identifying regions that will need to develop integrated pest management programs to manage Madeira mealybug, especially for some highly suitable areas, such as South Asia and Europe. Moreover, the results of this research will help governments to optimize investment in the control and management of the Madeira mealybug by identifying regions that are or will become suitable for infestations.