bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunohistochemical study of motoneurons of the lumbar spinal cord of mice after the 30-days space flight on biosatellite BION-M1
Authors: Tyapkina O. V , Rezvyakov P. N , Nurullin L. F , Petrov K. A , Nikolskiy E. E , Islamov R. R
Published: October 2024

We made immunohistochemical study of the lumbar spinal cord of c57black/6 mice after a 30-days space flight using antibodies against proteins which involved in providing of synaptic transmission (synaptophysin and PSD95). Also we use antibodies against proteins of intracellular protection (Hsp25 and Hsp70), and neuroprotective protein (VEGF) and its receptor (Flt-1). We found, that the levels of synaptophysin immune expression decreased by 21%, PSD - by 55%, Hsp25 - by 15% and Hsp70 - by 9% in mice lumbar spinal cord motoneurons after space flight. Thus, there were no significant changes in the immune expression of VEGF and its receptor Flt-1. Obtained data reveal the changes in the functional state (synaptic activity and resistance to stress) of mice lumbar spinal cord motoneurons after space flight. So, we have shown a new data about spinal cord motoneurons role in the development of hypogravity motor syndrome.
Activity index of lymphocyte apoptosis in children with neonatal sepsis
Authors: Khaertynov Kh. S , Boichuk S. V , Anokhin V. A , Ramazanov B. R , Dunaev P. D , Khaiboullina S. F , Rizvanov A. A , Andreeva A. A , Satrutdinov M. A
Published: October 2024

We studied the lymphocyte apoptosis in 15 infants with late neonatal sepsis, whereas 8 of them (53.3%) were considered as a preterm infants. Etiology of sepsis was identified in 4 cases (26.7%): in 1 case of each, the cause of the disease was Klebsiella pneumoniae, Pseudomonas koreenses, Candida and St. agalactae. Control group consisted of 5 healthy newborns. Apoptosis analysis was conducted by measuring the amount of hypodiploid cells by using a propidium iodide (Sigma Aldrich) DNA staining procedure and flow cytometry (FACsCanto II, Becton Dickinson). We observed an elevated numbers of apoptotic cells in all cases of neonatal sepsis. The most significant difference was observed when the lymphocytes were cultured for 3 and 5 days. For example, on day 3 of culture the numbers of apoptotic cells in patients with neonatal sepsis vs control group were 19.6% and 5.13%, respectively. Absolute lymphopenia was noted in 26.7% of cases with neonatal sepsis. No difference in the lymphocyte apoptosis between term and preterm infants was observed. An increased amount of lymphocyte apoptosis was not associated with C-reactive protein level during the whole time-points of the experiment: at 1, 3 and 5 days. Acute phase of neonatal sepsis is associated with an increased apoptosis of peripheral blood lymphocytes.
The spread of mobile genetic elements SCCmec-type strains Staphylococcus Aureus, isolated with skin and mucous of patients with allergic pathology
Authors: Shamsutdinov A. F , Toymentseva A. A , Tyurin Yu. A , Bayazitova L. T
Published: October 2024

Staphylococcus aureus is widespread bacterium that causes inflectional diseases of humans and animals. Currently, there is growing incidence of methicillin-resistant strains of Staphylococcus aureus. Resistance of Staphylococcus aureus strains to methicillin is detected by the presence in the genome of staphylococcal chromosomal cassette of SCCmec type. In this work 60 clinical strains of MRSA isolated from the skin and mucous of patients with allergic pathology were examined. The analysis of Staphylococcus aureus strains was performed in differential mediums. Additionally, specific identification was carried out by MALDI-TOF. Specific regions of the genes of mec gene complex were identified by PCR. As a result of typing we determined that 47% of tested strains were resistant to oxacillin phenotypically, but did not contain the specific primer sites related to mec- gene complex types I-IV. The remaining 53% of the strains were contained primer-specific amplicons. These strains were also phenotypically resistant to oxacillin. This study showed that MRSA strains as part of its genetic apparatus are several different types of SCCmec cassettes. This property allows them to express multidrug resistance.
Geneand cell-based therapy of muscle system hereditary disorders: state-of-art
Authors: Deev R. V , Mavlikeev M. O , Bozo I. Ya , Pulin A. A , Eremin I. I
Published: October 2024

Genetic disorders primarily affecting skeletal muscles can be caused by dysfunction of more than 30 genes. To date there is no effective etiotropic and pathogenetic treatment of such disorders. Investigators focus on search for new therapeutic agents based on gene and cell technologies, small molecules as well. There are numerous preclinical and several dozens of clinical studies in the world. Unfortunately tested technologies did not lead to significant advance in treatment of patients with such disorders. At the same time resulting data allow to determine the most feasible directions of future development - combining of genome correction methods with cell delivery of corrected genome to skeletal muscles. This review is intended to give general information about etiology of skeletal muscles genetic disorders, the main directions of biotechnological development and results of the clinical studies.
Features of multipotent mesenchymal stromal cells derived from various intraoral sources
Authors: Bozo I. Y , Zorin V. L , Eremin I. I , Deev R. V , Drobyshev A. Y , Korsakov I. N , Pulin AA. A
Published: October 2024

Multipotent mesenchymal stromal cells (MMSC) derived from various intraoral sources attracts attention of an increasing number of researchers due to availability and some features making them different from MMSC of bone marrow or adipose tissue. The review describes the main intraoral sources, characterizes their anatomical, topographical and histological peculiarities which may influence on MMSC morphofunctional profile. In comparative aspect we systematized the principal data concerning properties of MMSC derived from gingiva, buccal mucosa; periodontal ligament, dental pulp, apical papilla, jaw periosteum. It is not possible to compare MMSC of various intraoral sources under some parameters because of absence appropriate experimental findings. Some additional difficulties are caused by contradictions in published materials that are discussed in the review as well.
Cytotoxic activity of plant oils of Nigella sativa, salvia officinalis and peptide metabolites of streptomyces mirabilis fK749 against HeLa and MCF-7 cancer cell lines
Authors: El-Shafei S. M.A , Abd El-Rahman A. A , Validov S. Z , Bikmullin A. G , Sanatova E. R , Zalyalyutdinova L. M , Fattakhova A. N , Alimova F. K
Published: October 2024

In the last several years, there has been an increase in the use of plant materials in clinical development of new drugs and methods in the treatment of neoplastic diseases. In this regard, the current study was aimed to investigate the cytotoxic activity of Nigella sativa (NS), Salvia officinalis (SO) plant oils and peptide metabolites (PM) of Streptomyces mirabilis FK749 on the viability of Hela and MCF-7 cancer cells. To achieve this objective, cells were exposed to 0, 20, 40, 60, 80 and 100 mg/ml of NS, SO plant oils and PM S. mirabilis FK749. After 24 h. incubation the effect of plant oils and PM on the viability and apoptotic activity of the cancer cells were assessed by using trypan blue and TMRE assays. Morphological changes were examined. Whereas, the half-maximal inhibitory concentrations of these oils and PM were assessed after 72 h. incubation. The results of this study showed that treatment Hela and MCF-7 cells with NS, SO and PM at 40 mg/ml and above concentrations up to 100 mg/ml were found to be cytotoxic and leads to a decrease in the cell viability. The morphological changes observed in Hela and MCF-7 cells exposed to different concentrations of NS SO and PM were found to be in a concentration dependent manner. Hela cells were shown to be more resistant against oil components and PM than MCF-7 cells.
Experimental and Morphological study of the xenogenic biological membranes
Authors: Ryabov A. Yu , Fadeeva I. S , Deev R. V , Vezhnina N. O , Yurasova Yu. B , Fesenko N. I , Guriev V. V , Sklyanchuk E. D , Lekishvili M. V , Akatov V. S
Published: October 2024

In the last decade barrier the application of different types of membranes in the oral and maxillofacial surgery has been increasing. Their main reliability features include resorption times, the lack of toxic and antigenic effects, the absence of negative effects from the membrane on the structure of the surrounding soft tissues, as well as a high degree of biointegration and a potentiality of steady stabilization in the wound. Considering the abovementioned parameters, graft barrier membranes are a material of choice in the modern reconstructive oral and maxillofacial surgery. The present study includes an experimental and morphological assessment of biologic xenogenic barrier membranes for the evaluation of their immunological potency and the time of biodegradation using a model test system in vivo. Three groups of barrier membranes were assayed - M-D (decellu-larized diaphragm), M-P (pericardium), M-DM (dura mater), prepared from the corresponding tissues of a mature ox, in accordance to the method developed in the Priorov Central Research Institute of Traumatology and Orthopaedics. The experiments were carried out on a model of 6-week heterotopic subcutaneous implantation in rats and included a complex histological and immunohistochemical analysis, with quantitative estimation of biograft mineralization by adsorption spectroscopy. It was found that: the material of M-DM has the highest and fullest (while retaining the strength properties) resorption; a relatively long bi-odegradation time, expressed conductive effect and constraint function are specific for the М-Р material; M-D membranes have faster resorption, enhanced thinning and relative biological inertness. The results of the conducted study indicate that all the implanted materials are biocompatible and efficient, and thus may be used in various medical cases.
Description of methods for the detection of Helicobacter pylori in children with chronic gastroduodenal pathology
Authors: Faizullina R. A , Abdullina E. V , Akhmetov I. I
Published: October 2024

Frequent detection of Helicobacter pylori infection among both adult and child population, as well as its relationship with the development of chronic gastroduodenal pathology field justified the need to search for sensitive and accurate diagnostic methods. This has stimulated the emergence of a variety of different methods for detecting Helicobacter pylori, which created for clinicians and specialists laboratory diagnosis difficulties in choosing the adequate and reliable ways to identify. A comparative analysis of methods for diagnosis of Helicobacter pylori infection in 151 children with chronic gastroduodenal pathology field, including 23 patients with duodenal ulcer, 97 patients with chronic gastroduodenitis and 31 conditionally healthy children of the same age. Breathing helic - test and polymerase chain reaction of gastric mucosa biopsies for the diagnosis of Helicobacter pylori infection have equally high sensitivity (94.8% and 93.6%) but low specificity (80.6% and 79.5%) and a high probability helicobakteriosis methods with negative results (19.4% and 18.4%).The analysis of the diagnostic value of the combination helic - test and polymerase chain reaction in the verification of Helicobacter pylori infection showed a higher sensitivity (98.3%) and specificity (93.5%) overall accuracy (96%) and the probability of a positive result helicobakteriosis (96.6%) and a low probability in a negative result (6.4%) methods. Therefore, for the primary diagnosis of Helicobacter pylori infection is better to use a combination of breathing helic -test and polymerase chain reaction biopsies gastric mucosa, reducing the proportion of false-positive and false-negative data, would increase the sensitivity and specificity of the methods.
Features of bone tissue regeneration after gunshot fractures of human long bones
Authors: Gololobov V. G
Published: October 2024

The goal of the study was to analyse the features of tissue reactions during reparation of the bone wound following gunshot long bone fracture in human. Analysis of biopsy specimens, i.e. bone splinters, edges of bones fragments and surrounding them tissues taken from 9 male patients undergoing surgical treatment due to gunshot fracture of long bones has been carried out. Histological study using light and electron microscopy has been performed on the 1st, 2d, 3d, 4th, 5th, 14th, 23d and 34th days following the injury. It has been shown that newly developed blood vessels are capable for transportation of osteogenic cambial cells into the zone of the fracture thus optimizing osteogenesis. Regenerative endossal osteogenesis found out in this study proved to promote reconstructed osteons formation missing a stage of reticulofibrous bone tissue. It has been shown also that cells carrying osteogenic properties introduced into retained splinters during re-vascularization are responsible for the bone tissue construction. Such fragments served as additional and considerable source of bone regenerate formation. They are considered to be so-called «post-injury organ culture» in vivo. Difference between development of regeneration zone from distal and proximal edges of fractured bones was documented. Ultrastructural changes within osteoblasts, osteocytes and extracellular matrix reflected the intensity of bone tissue formation. Our results have been taken into consideration in routine clinical management and methods of treatment among such patients have been proposed.
Recovery options of reproductive function of cancer patients due to transplantation of cryopreserved ovarian tissue
Authors: Abakushina E. V , Otoi T. , Kaprin A. D
Published: October 2024

Cancer patients survive at increasing rates, but successful treatment in younger patients often leads to reduced fertility. If damage to reproductive organs from treatment is unavoidable, cryopreservation of ovarian tissue can protect fertility for young patients prior to treatment. Well known that the mammalian ovary contains a huge stock of resting оосуtes. The large store of these small follicles creates a potential source of oocytes for fertilization. To utilize the potential female gametes stored in ovaries, it will be important to safe ovarian tissue before oocytes undergo degeneration during treatment of cancer cryopreservation and transplantation of ovarian tissue are two emerging techniques for fertility preservation, especially in yang cancer patients If these technologies are to become widely accepted, they need to be safe, easy to perform and must obtain favorable result. Recent advances in cryobiology have made it possible to preserve ovarian tissue with relatively little loss of viability. If gonadal toxicity of chemo radiotherapy is unavoidable, physicians also should be knowledgeable about options for fertility preservation and offer patients a referral to a fertility specialist. The ability of having genetically related children is an important issue for patients surviving cancer.
Academician A.A. Zavarzin scientific progeny of histologists-evolutionists
Authors: Gololobov V. G
Published: October 2024

The 130th anniversary of academician A.A. Zavarzin, the founder of evolutional histology and the author of fundamental theory of parallel series of tissue development will held in 2016. Being an outstanding scientist and organizer, he managed to create a powerful school of histologists-evolutionists in the Soviet Union. The paper contains short variant of history foundation and analysis of the most outstanding achievements of his school, which are contributing towards perspective development of histology
Technologies of cellular antitumor immune response induction in vitro
Authors: Sennikov S. V , Kulikova E. V , Obleukhova I. A , Shevchenko J. A
Published: October 2024

Dendritic cells are “professional” antigen-presenting cells and the most potent stimulators of various immune responses of the organism, including antitumor. Modern studies have shown that an effective antitumor immune response doesn't occur in patients with malignant tumors. This is largely due to a decrease in functional activity of dendritic cells in cancer patients through irregularities in the maturation process to a functionally active form and in the antigen presentation process to naive T lymphocytes This review describes the main stages in technology of cellular antitumor immune response induction in vitro, aimed at resolution of the problems blocking the full functioning of dendritic cells, and additional stimulation of antitumor immune response, as well as prospects for the technology development
Prospects of the tissue engineering lung development with the methods of regenerative medicine (review)
Authors: Kuevda E. V , Gubareva E. A , Sotnichenko A. S , Gilevich I. V , Gumenyuk I. S , Macchiarini P.
Published: October 2024

Deficit of donor organs, the need for lifelong immunosuppressive therapy, even in the case of successful lung transplantation, high risk of death in the postoperative period are forced to look for the new ways to treat terminally ill patients, requiring organ transplants. In the last decade, much attention is given to methods of regenerative medicine to repair or replace the function of damaged tissues and organs. Creating functional lungs in the laboratory will hopefully solve the problem of donor organs shortage. The study of morphological properties of biological scaffolds, deeper understanding of stem cells and progenitor cells behavior led to the idea of using decellularization methods followed by recellularization with autologous cells for tissue engineered trachea, lungs, heart and kidney creation Recellularized solid organs can perform organ-specific function in vitro conditions, indicating the potential clinical use of these methods This review presents the current data about lung decellularization and recellularization methods to increase efficiency and improve the quality of the biological scaffold and discusses the main aspects of lung transplantation in animal models and perspectives of lung bioengineering
Investigating the properties of membrane vesicles obtained from human cells HEK293 using cytochalasin B
Authors: Gomzikova M. O , Rizvanov A. A
Published: October 2024

The preparation method of membrane vesicles from human cells using cytochalasin B allows to overcome the limitations of human cells natural microvesicles, associated with the complex procedure of isolation and limited output. Membrane vesicles (MV) prepared from human cells are a promising vector for delivering of various bioactive substances. We performed the preparation of MV from human cells HEK293 using cytochalasin B and size determination of the MV. Then we studied the influence of applied MV concentration and intravesicular substance concentration on the substance delivery effectiveness to recipient cells. It was found that MV ranging in size from 164.2 nm to 3580 nm, but the most of MV sized from 164.2 nm to 712,4 nm (84. 6%). MV are able to enclose the cytoplasmic contents of the parent cells and deliver it to recipient cells, the amount of delivered substance (CFDA SE) to the recipient cells is proportional to the loaded substances into MV
Lekishvili Experimental and morphological study of the bone plastic materials for surgical treatment of ENT pathology
Authors: Ahmedov Sh. M , Musina L. A , Kocharyan E. Z , Ryabov A. Yu , Lekishvili M. V
Published: October 2024

To study the possibility of application different materials during plastic surgery of the middle ear structures with background of purulent infection experimental model with cranial bone defects replacement was utilized. As the object of the experiment 17 rabbits «Chinchilla» were used. All animals underwent surgery with formation of artificial defects in parietal bones (diameter of 7 mm) followed by simultaneous grafting. Following the implantation of plastic materials animals underwent standard antibacterial and local anti-inflammatory therapy For the plastic of parietal bone defects at rabbits were used and studied: autologous plates from the parietal bone and the iliac crest, auricle's autologous cartilage plates, demineralized bone implants (DBI), derived with technology of producing osteoplastic material «Perfoost» (CITO) from cortical long bones, rabbit's auricle autologous cartilage preserved in 0. 2% thymol solution, synthetic plastic material, comprising a hydroxyapatite and antibiotic - “Collapan-D” and osteoplastic composite material obtained by adhesion of the autologous chips from iliac crest and the parietal bone of rabbits with fibrin bond “Byo-Glu”. Timing of the experiment consisted of 15 and 30 days, 3 and 6 months By 3 months of the experiment organotypical callus were obtained in cases of application pure autologous tissues (plates from rabbit's parietal and the iliac crest and, as well, as auricle's autologous cartilage plates) Similar results were obtained in the cases with DBI “Perfoost” and allogenic rabbit's auricle cartilage preserved in 0. 2% thymol solution. In the defects filled with “Collapan-D” and combined osteoplastic material with fibrin glue «Byo-Glu» at 6 months of the experiment most part of the material was resorbed and partially replaced by newly formed bone alternating with areas of connective tissue and remnants of implants
Etiopathogenesis and principles of myasthenia gravis treatment
Authors: Kharlamova A. D , Petrov K. A , Zueva I. V , Nikolsky E. E
Published: October 2024

The review addresses issues of etiological studies of synaptic defects underlying severe immunological disease of neuromuscular system myasthenia gravis. Current concepts on molecular mechanisms of muscle weakness accompanying this disease are considered, development of new algorithms of myasthenia gravis treatment is discussed
The human dermal fibroblasts cultivation on the porous polytetrafluoroethylene membrane for a scleroplastic
Authors: Blinova M. I , Yudintceva N. M , Vershevskaja E. A , Dghanaeva Z. N , Astakhov Yu. S , Tomson V. V , Potokoin I. L , Galibin O. V , Pinaev G. P
Published: October 2024

The cell technologies began to apply in ophthalmology actively. The aim of this investigation is to test the porous polytetraphtorethilen membrane as a substrate for the human dermal fibroblasts growth with further using for scleroplastik. It was shown by the scan electron microscopy that this membrane is a suitable substrate for a dermal fibroblast growth. Also it was shown, that the tissue-like structures are formed on the membrane previously seed with cells formerly than on membrane without cells in result of this membrane implantation into rabbit eye The obtained results suggest that dermal fibroblasts growing on porous polytetraphtorethilen membrane may be used in ophthalmology for the restoration of the damage or losed tissues. The clinical example mentioned in this work is evidence in this conclusion
Some physicochemical and biological characteristics of 3D printed constructions based on sodium alginate and calcium phosphates for bone defects reconstruction
Authors: Sergeeva N. S , Komlev V. S , Sviridova I. K , Kirsanova V. A , Akhmedova S. A , Shanskiy Ya. D , Kuvshinova E. A , Fedotov A. Yu , Teterina A. Yu , Egorov A. A , Zobkov Yu. V , Barinov S. M
Published: October 2024

A creation of personalized constructions for tissue engineering of bone tissue is very perspective biomedical technological trend. An aim of our work was studying physicochemical characteristics, cyto- and biocompatibility of 3D printed constructions based on sodium alginate and three calcium phosphates species (tricalcium phosphates, carbonated hydroxyapatite, and octacalcium phosphate). The methods of 3D constructions producing included 3D printing of components with crosslinking agent (CaCl2), their freezing, sublimation, “forced shrinkage”, γ-ray sterilization (15 kGr). A structure of 3D constructions, their porosity and strength characteristics were studied. The cytocompatibility of 3D constructions and matrix-for-cell properties were investigated in vivo on a model of human osteosarcoma MG-63 cell line by means of MTT assay. The biocompatibility of 3D constructions was studied on the model of subcutaneous implantation in mice up to 12 weeks Using scanning electron microscopy it was found that all 3 types of constructions have a lamellar structure of alginate component with spherical inclusions of calcium phosphate Total porosity of species was 54,5-63,9%. Calcium phosphates in the constructions reserved initial phase composition Compressive strength of 3D constructions depended on inorganic component and was 1,8-3,7 MPa with ultimate strain 12,3-12,6%. All types of 3D constructions were cytocompatible in vitro, demonstrated good matrix-forcells properties, and had supported cell proliferation for 2 weeks. in results of subcataneous in vivo test all constructions demonstrated biocompatibility with slow bioresorption of organic and inorganic components. Received data proved the promising outlook for further improvement of 3D printing and investigations of described 3D constructions as osteoplastic materials
Nanodiamond composite scaffolds for human skin fibroblasts cultivation
Authors: Nashchekina Yu. A , Margulis B. A , Gordeev S. K , Blinova M. I , Guzhova I. V
Published: October 2024

Carbon materials are widely evaluated as scaffolds for cultured cells in “regenerative” bone surgery. In this research we investigated the interaction of human skin fibroblasts with diamond carbon composites scaffolds. Scaffolds with 10-100 nm pore diameter were obtained from the dispersed powders of diamond with particle size - from 2 nm to 5 microns It was shown that all scaffolds were not toxic for cultured cells. The highest number of cells adhered on the scaffolds with average pore size of 50-100 nm Scaffold with a pore size of 50 nm contribute to the fibroblasts parallel orientation of their surfaces
Apoptosis-inducing activity of Bacillus pumilus ribonuclease and some egyptian medicinal plants extracts on human alveolar adenocarcinoma cells
Authors: Karamova N. S , Zelenikhin P. V , Miroshnik N. B , Essam Abdul-Hafeez , Zakirova Ya. N , Ilinskaya O. N
Published: October 2024

Induction of apoptosis is a primary mechanism of anticancer activity of several drugs using for cancer therapy. Apoptosis-inducing activity of Bacillus pumilus ribonuclease (binase) and stem bark aqueous extracts of eight medicinal plants from Egypt in human A549 alveolar adenocarcinoma cells was studied. It was shown that binase (300 μg/ml) significantly increases the portion of apoptic cells in population after 24 h. Extracts of Albizzia lebbeck and Bauhinia variegate demonstrated a clear dose-dependent apoptosis-inducing effect. Combined treatment of A549 cells with binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana enhances induction of apoptosis in comparison with binase and extracts alone. Results obtained allow to consider combination of binase and stem bark aqueous extracts of Albizzia lebbeck, Bauhinia variegate, Kigelia africana as a source for development of low toxic, natural drugs to induce apoptosis in tumor cells
Transplantation of cell sheets from adipose-derived mesenchymal stromal cells effectively induces angiogenesis in ischemic skeletal muscle
Authors: Makarevich P. I , Boldyreva M. A , Dergilev K. V , Gluhanyuk E. V , Gallinger J. O , Efimenko A. Yu , Tkachuk V. A , Parfyonova Ye. V
Published: October 2024

Delivery of cells is a promising approach to induce blood vessel formation for treatment of ischemia. Still, efficacy of these methods has been shown to be below expectations due to the fact that injection procedures used to transplant cells can diminish their survival rate. To circumvent this problem a technique known as “cell sheets” can be utilized. Cell sheets are minimal tissue-engineered constructs that comprise of cells along with their extracellular matrix proteins Present study investigates application of cell sheets from adipose-derived mesenchymal stromal cells (AD-MSC) to stimulate angiogenesis. In a mouse model of limb ischemia we demonstrate that subcutaneous implantation of a cell sheet from 1 mln AD-MSC effectively stimulates angiogenesis and restores perfusion of ischemic muscle compared to untreated animals with limb ischemia. Histology also indicates that cell sheet transplantation results in decreased necrosis of skeletal muscle and retain of AD-MSC at Day 14 with certain prevalence of proliferating and minimal amount of apoptotic cells within cell sheet Furthermore, comparison of cell sheet-treated animals vs. injection of the same dose of AD-MSC shows that cell sheet delivery was superior to routine injection-based delivery in terms of limb perfusion and tissue protection Obtained results indicate that local application of AD-MSC cell sheets to promote angiogenesis and protect skeletal muscle from ischemia can be a promising approach for therapeutic use
Antitumor effect of hybrid nanocomplexes containing nanoparticles of orthovanadates rare earth elements and cholesterol
Authors: Goltsev A. N , Babenko N. N , Gaevskaya Yu. A , Chelombytko O. V , Bondarovich N. A , Dubrava T. G , Ostankov M. V , Dimitrov A. Yu , Klochkov V. K , Kavok N. S , Malyukin Yu. V
Published: October 2024

An actual task of current oncology is the search for the structures, enabling to selectively affect the tumor stem cells, on the expansion rate of those the activity of oncologic process depends. Perspective in this aspect is the use of nanostructures selectively recognizing and inactivating tumor stem cells. In the research there was studied the effect of synthesized hybrid nanocomplexes based on nanoparticles of rare earth orthovanadates GdYV04:Eu3+, cholesterol and hydrophobic luminescent stain Dil on functional activity of Ehrlich carcinoma cells and expression in them of nanog, oct-4, sox-2 genes After pre-treatment of Ehrlich carcinoma cells with nanocomplexes there was shown an inhibition of tumor growth in vivo due to inactivation of the most carcinogenic CD44hi cells, which was accompanied with the reduced expression rate of the studied genes in total pool of cells The findings contribute to the understanding of the effect mechanisms of orthovanadates and open the prospects to apple new forms of nanocomposites in treatment of oncology diseases
Analysis of Bj fibroblasts mitochondrial respiratory chain function under glucose starvation and exposure to different doses of rotenone: Implications for neurogenerative diseases
Authors: Ivanova V. V , Starostina I. G , Martynova E. V , Pereira S. P , Oliveira P. J , Rizvanov A. A
Published: October 2024

Certain neurodegenerative diseases, such as Parkinson and Alzheimer, are characterizing by an impairment in mitochondrial function and biogenesis, which may lead to pathological changes in the central nervous system. From this putative link stems a growing interest in changes to the mitochondrial electron transport chain and the ensuing energy dysfunction in neuronal cells and connective tissue cells under normal and pathological conditions Fibroblasts involved in the formation of microenvironments of different types of specialized cells from the nervous system and their dysfunction may contribute to the pathogenesis of disease as well. In this regard, we have obtained stressful conditions of human dermal fibroblasts approximating of the pathological phenotype observed in Parkinson's disease. Was studied the expression and activity of the protein complexes of the mitochondria respiratory chain including translocase TOM20 under inhibition of NADH dehydrogenase and maintaining of oxidative stress
Effect of decellularization with sDs and Triton X-100 detergent solutions on the strength capacities of small caliber arteries
Authors: Akhmedov Sh. D , Lugovsky V. A , Andreev S. L , Rebenkova M. S , Rogovskaya Y. V , Skurihin I. M , Vecherskiy Yu. Yu , Afanasyev S. A
Published: October 2024

The research is dedicated to evaluation of the effect of the human mammary artery decellularization with 1 % solution of sodium dodecyl sulfate (SDS) and 1% solution of Triton X-100 during 2 hours on its morphological structure and its strength capacities As a result of decellularization all the cellular elements were eliminated from the vessel. Nevertheless, it appeared to be possible to preserve complex spatial structure of the vascular wall. The process of decellularization did not change significantly collagen basis of the vascular wall Although, under 2 hour effect of the detergents one could see lowering collagen concentration down to 80% in intima, media and adventitia. However, even in these circumstances the strength of the vessel was not less than 46% versus that of intact vessels The obtained data are important for the further study aimed at the development of a new generation of vascular implants
Conduit based on poly(ε-caprolactone) filled with fibrin-based hydrogel with mesenchymal stromal cells for peripheral nerve defect reconstitution
Authors: Mukhamedshina Y. O , Masgutov R. F , Masgutova G. A , Zuravleva M. N , Shulman A. P , Galieva L. R , Rogozin A. F , Chelyshev Y. A , Rizvanov A. A
Published: October 2024

In this study we used promising therapeutic strategy for stimulation of peripheral nerve regeneration - implantation of poly(ε-caprolactone) nerve conduit filled with fibrin hydrogel Tissucol in combination with mesenchymal stem cells. In vitro studies showed that the coating by Tissucol of poly(ε-caprolactone) substrate promotes cell proliferation. In vivo results of peripheral nerve defect reconstitution in rats using the conduit based on poly (ε-caprolactone) filled with fibrin hydrogel with mesenchymal stem cells confirmed the effectiveness of the proposed approach
Tryptophanyl-tRNA synthase gene expression as genetic marker of the athletes’ overtraining
Authors: Nurbekov M. K , Elov A. A , Il'in A. B , Ibragimova M. Y , Zhdanov R. I
Published: October 2024

In connection with a necessity the development of effective biomarkers of sportsman physical and overtraining (distress) states, the study of a role of triptophanyl-tRNA synthase, TRSase, and corresponding gene in process is of special interest. Preliminary estimate of specificity and level of mRNA expression of TRSase gene is carried out using method 1: scanning of gels with an image of separation of polymerase chain reaction PCR products to detect quantitatively the content of the mRNA and/or cDNA copies compare, in parallel, to DNA quantity markers to compose a calibrating curve. Final estimation of TRSase gene expression is carries out by real time PCR (method 2) using calibrating curve (technique 1) and relative quantitative estimation of a number of specific mRNA copies with reference gene involved (technique 2). The level of TRSase gene expression represents a criterium of organism response, which is adequate to a stress strength (overtraining) It is proposed to detect the overtraining state at sportsmen using registrating an increased TRSase gene expression. In our pilot project, the distress overtraining state is determined as increased level of specific TRSase gene expression in 1,45 fold higher at the samples under the study compare to control samples. The TRSase mRNA expression before training is increased by 1 2-1 6 fold compare to one after training
Action of NMDA receptors agonists on amplitude of miniature endplate potentials in PRiMA and ColQ genes knockout mice
Authors: Proskurina S. E , Petrov K. A , Kharlamova A. D , Krejci E. , Nikolsky E. E
Published: October 2024

Previously it was shown that activation of NMDA receptors at neuromuscular junction can enhance nitric oxide (NO) production. NO, in turn, able to inhibit synaptic acetylcholinesterase activity and increases amplitude of synaptic responses In this study we tested a hypothesis that molecular form of acetylcholinesterase anchored in plasma membrane could be more sensitive to endogenous NO inhibition than molecular form of acetylcholinesterase located on basal lamina Experiments were performed on extensor digitorum longus of (-/-) PRiMA, (-/-) ColQ and wild type mice Miniature endplate potentials were recorded using standard microelectrode technique. After application of NMDA receptors agonists (glutamate and glycine) amplitude and decay time of miniature endplate potentials did not change in synapses of PRiMA or ColQ knock-out mice. Obtained results show that nitric oxide production owing to the activation of enzyme NO-synthase, which is initiated by glutamate mediated excitation of synaptic NMDA receptors, does not affect selectively one of molecular forms of acetylcholinesterase
Efficiency of IL-2 and IL-15 combined use for activation of cytotoxic lymphocytes in vitro
Authors: Abakushina E. V , Marizina Yu. V , Neprina G. S
Published: October 2024

0ne of the modern approaches for cancer treatment is based on the application of immunotherapy using activated cytotoxic lymphocytes. Search of methodological approaches for the preparation of activated lymphocytes in vitro is relevant. As a result of this study, the method for activation and culturing of lymphocytes for cancer patients have been perfected using cytokine IL-2 and IL-15. Peripheral mononuclear cells of cancer patients were culture using two different mediums based on RPMi-1640 with IL-2 for 10 days and X-vivo20 supplemented with IL-2 and IL-15 for 14 days. The expression of activation markers (CD38, CD69, CD25, HLA-DR and CD314) and subpopulations of lymphocytes were evaluated by the method of flow cytometry every 2 days. The expression of activation markers of lymphocytes increased after 3 days of culture in the first medium and after 5 days in the second one. We revealed that the activation of lymphocytes was faster in medium based on RPMI with IL-2, but the proliferation and viability of lymphocytes were lower than in the second medium. The culture medium based on RPMI with IL-2 can be recommended for more quickly obtaining of lymphokine-activated killer cells. The medium based on X-vivo20 with a combination of IL-2 and IL-15 can be recommended for a longer cultivation of lymphocytes and for escalating of lymphokine-activated killer cells. it has been shown that the combination of cytokines IL-2 and IL-15 not only has a positive influence on the proliferation activity of the lymphocytes and the expression of activation markers, but also on their viability.
Nucleic acid-protein identification: novel protein classification based on nucleic acid-protein interaction
Authors: Krylov A. S , Zhdanov R. I
Published: October 2024

Several basic properties of proteins are used for their classification. Some proteins can bind DNA and RNA in a specific way. We propose a simple method for classification of nucleic acids binding proteins which we call «nucleic acid-protein fingerprint» The method is based on usage of short oligonucleotides, immobilized in hydrogel for hybridization with proteins. First, we have found the shortest oligonucleotide capable for specific binding with nucleic acids. Experiments with samples from 2 to 12 bases length have shown that tetramers with 2 chains of 5-nitroindole (universal base) and 2 chains of normal bases can recognize proteins. Thus, we have made a smallest universal protein recognizing biochip containing 16 dinucleotides. Physical length of them is 4 chains, two chains are from 5-nitroindole and two chains are normal DNA bases We manufactured that biochip and investigated binding of dye labeled proteins with them. The hybridization patterns of two proteins RNase A and Binase have shown remarkable difference specificity and difference between proteins Biochip of this type with three of four remarkable proteins will have much more specificity. Thus, we proposed and experimentally confirmed a new type of proteins classification
Impact of histological material fixation on the results of immunohistochemistry
Authors: Vaganova A. N
Published: October 2024

A common method of tissue fixation is the fixation in formalin. This fixing method is related to the chemical transformations of molecules and may influence the stability of their epitopes. The extent of this impact is aggravated by prolonged tissue stay in the fixative. To standardize the result of research, it should thus be standardized length of tissue fixation. The allowable fixation delay after the separation of tissue from a circulatory system also must be limited. However, in the modern time, the new requirements for the time to result and preservation of the molecular composition in the tissue leads to introducing of new technologies and fixing reagents into the practice There is a trend to move to coagulating fixatives, based on the various alcohols. The impact of such reactives on biomolecules in general is gentler. The feature of the protein antigens as an object of study is a high diversity of chemical structure that dictates the necessity for an individualized approach to development of immunohistochemical staining protocol including factors that the tissue meets before the staining procedure. Such optimization procedure is carried out mainly empirical. Existi ng immunohistochemical tests are adapted for the formalin-fixed tissue, and their use after the fixation in other conditions requires preliminary studies for protocol adaptation and optimization
Pharmacotherapy effect on genetic diversity of intestinal biocenosis
Authors: Safina D. D , Abdulkhakov S. R , Grigoryeva T. V , Markelova M. I , Ismagilova R. K , Khusnutdinova D. R , Malanin S. Yu , Laikov A. V , Siniagina M. N , Abdulkhakov R. A , Chernov V. M
Published: October 2024

Diarrhea is one of the most frequent adverse events of H. pylori eradication therapy due to a violation of the intestinal microflora The aim of the study was to assess intestinal microflora content in H. pylori-positive and H. pylori-negative patients, as well as the effect of eradication therapy on its qualitative and quantitative composition 78 stool samples were used for analysis: 34 samples from H. pylori-positive patients before eradication therapy, 34 - from the same patients after completion of eradication therapy, 10 samples from H. pylori-negative patients (control group) A total deoxyribonucleic acid (DNA) was isolated from the stool samples by phenol extraction method, a nucleotide sequence of the isolated DNA was established by shotgun sequencing method Composition of intestinal microflora community was evaluated based on the number of species, the qualitative composition and diversity Shannon index. Statistical analysis and visualization of the analysis results were carried out in R medium Bacteroides, Prevotella, Eubacterium, Roseburia, Faecalibacterium and Clostridium were predominant genus in all groups of faecal samples, however, representation variability of the prevailing phyls (Bacteroides and Firmicutes) was lower in the control samples than in H. pylori-positive patients before and after eradication therapy Eradication therapy resulted in reduction of the Coprococcus, Bifidobacterium, Collinsella genera representation and increase in the number of Clostridium, Bacteroides, Coprobacillus and Flavonifractor. In about half of the patients eradication therapy leads to a decrease of both the number of species and the Shannon index, which indicates a decrease in the overall diversity and, consequently, reduction of the stability of community, with a possible predominance of individual species. These results suggest that changes in the intestinal microflora composition after H. pylori eradication therapy are individual and caused by initial condition of the intestinal microbiota with a predominance of its particular representatives
Supravital lanthanoid staining for scanning electron microscopy of biological objects
Authors: Novikov I. A , Subbot A. M , Fedorov A. A , Griboedova I. G , Antonov E. N , Vakhrushev I. V
Published: October 2024

Since modern scanning electron microscopes are capable of low-vacuum mode and back-scattered electron detection, no other sample preparation, except for heavy metal staining, is necessary. Rare earth chlorides were used as contrast agents to study ocular tissues, cell cultures, and small invertebrates. It has been shown that supravital lanthanoid staining, if adjusted to meet the requirements of modern technology, significantly increases contrast of back-scattered electron images along with their informative value. Not only the microrelief but also internal structures (5-10 μm beneath the surface) of the samples can be visualized without 'classical' sample preparation. Selective accumulation of lantanoids in cell membranes is likely to be due to their binding to calcium ATPases. The developed method of lanthanoid staining enables the use of scanning electron microscopy for subsurface examination of biological objects
Current methods in experimental angiogenesis investigation
Authors: Livanova A. A , Deev R. V , Rizvanov A. A
Published: October 2024

Growing interest in angiogenesis, a key component in the development of different diseases, requires the use of a suitable experimental model to simulate neovascularization in a laboratory. In recent years, with the development of novel therapeutic strategies, based on angiogenesis regulation, this problem has become especially important. Current in vitro and in vivo models are characterized with a variety of disadvantages, which impede results interpretation. Thus, in vitro assays provide estimation of discrete endothelial cells characteristics, which alter from the same ones in the native microenvironment. The use of in vivo assays is accompanied with difficulties in testing agent delivery and quantitative analysis of its angiogenic activity In view of these complications, the use of a combination of assays is recommended while planning the experiment in this area. The aim of this review is to critically analyze angiogenesis assays, currently used to perform fundamental investigation as well as preclinical tests of developing therapeutic agents
Effect of tescalcin overexpression on osteogenic differentiation of human mesenchymal stem cells
Authors: Solovyeva V. V , Kolobynina K. G , Gomzikova M. O , Tazetdinova L. G , Zhuravleva M. N , Slepak V. Z , Rizvanov A. A
Published: October 2024

Tescalcin plays an important role in the proliferation and differentiation of certain cell types. It is involved in the signaling pathways of mitogen-activated protein kinase and is reported to directly interact with subunit 4 of COP9 signalosome and Na/H exchanger NHE1. Since tescalcin is one of the factors that allow one type of progenitor cells differentiate into a variety of specialized cell types, it can be a potential molecular tool for modulation of phenotype of target cells. The ability of adipose-derived mesenchymal stem cells (ADSCs) to differentiate into osteogenic direction makes them a promising adult stem cell type for regenerative medicine and tissue engineering. Here we show that ADSCs with tescalcin overexpression had a significantly higher level of matrix mineralization compared with control ADSCs. This finding indicates that ectopic tescalcin overexpression might affect osteogenic differentiation of ADSCs.