bluuebunny/crossref_metadata_2025 huggingface dataset:
Optoelectronic Simulations of InGaN-Based Green Micro-Resonant Cavity Light-Emitting Diodes with Staggered Multiple Quantum Wells
Authors: Hsieh Tsau-Hua , Huang Wei-Ta , Hong Kuo-Bin , Lee Tzu-Yi , Bai Yi-Hong , Pai Yi-Hua , Tu Chang-Ching , Huang Chun-Hui , Li Yiming , Kuo Hao-Chung
Published: March 2023

In this research, we compared the performance of commercial μ-LEDs and three-layered staggered QW μ-LED arrays. We also investigated the self-heating effect. We proposed a green micro-resonant cavity light-emitting diode (µ-RCLED) that consists of a three-layer staggered InGaN with multiple quantum wells (MQWs), a bottom layer of nanoporous n-GaN distributed Bragg reflectors (DBRs), and a top layer of Ta2O5/SiO2 DBRs. We systematically performed simulations of the proposed µ-RCLEDs. For the InGaN MQWs with an input current of 300 mA, the calculated wavefunction overlaps are 8.8% and 18.1% for the regular and staggered structures, respectively. Furthermore, the staggered MQWs can reduce the blue-shift of electroluminescence from 10.25 nm, obtained with regular MQWs, to 2.25 nm. Due to less blue-shift, the output power can be maintained even at a high input current. Conversely, by employing 6.5 pairs of Ta2O5/SiO2 DBRs stacks, the full width at half maximum (FWHM) can be significantly reduced from 40 nm, obtained with ordinary µ-LEDs, to 0.3 nm, and a divergence angle smaller than 60° can be obtained. Our simulation results suggest that the µ-RCLEDs can effectively resolve the wavelength instability and color purity issues of conventional µ-LEDs.
Magnetic Field-Dependent Microstructure Evolution of Solidified Co39.2Ni39.2Al21.6 Eutectic Medium-Entropy Alloy
Authors: Liu Haoxiang , Bu Fan , Wu Yuhao , Xing Chenxu , Liu Xudong , He Yixuan
Published: March 2023

A (Fe, Cr)-free Co39.2Ni39.2Al21.6 eutectic medium-entropy alloy (EMEA) was designed and fabricated to study the microstructure and its evolution during slow solidification under different intensities of high static magnetic field (0 T, 5 T and 10 T). It was found that the original microstructure was characterized by FCC/BCC mixed herringbone eutectics consisting of two types of lamellar structures: a curved and wormy anomalous eutectic in the fringe, and a straight and long regular eutectic in the center. Nano-sized L10-type martensite layers are also distributed on the BCC lamellar as the martensitic transformation product. The FCC and BCC phases were enriched in Co and Al elements, respectively, while Ni element was distributed homogenously in both phases. With increasing magnetic field intensity, the herringbone eutectic structures remained stable, without the formation of a primary phase, while the phase constitution and the orientation relationships in the eutectic structures remained unchanged, with no obvious magnetically induced alignments. However, the lamellar spacing of the regular lamellar eutectic decreased significantly from 3.3 μm (0 T) to 1.93 μm (10 T); by contrast, the volume fraction of the anomalous eutectics increased considerably from 28.35% (0 T) to 55.14% (10 T), and the assumption that the imposed convection and destabilization of lamellar eutectics is controlled by the magnetic field is discussed in depth. Our results show a great potential for tailoring microstructures and properties by applying a strong magnetic field during the solidification process of EMEAs.
Preparation of HfNbTiTaZr Thin Films by Ionized Jet Deposition Method
Authors: Skočdopole Jakub , Čech Jaroslav , Čapek Jiří , Trojan Karel , Kalvoda Ladislav
Published: March 2023

The ionized jet deposition (IJD) method is applied to the preparation of thin films composed of refractory HfNbTiTaZr high-entropy alloy (HEA). Due to its stoichiometric reliability, the IJD method provides a flexible tool for deposition of complex multi-element materials, such as HEAs. Scanning electron microscopy, energy-dispersion spectroscopy, confocal microscopy, and X-ray diffraction methods are used to characterize the influence of the applied accelerating voltage of the IJD deposition head ranging from 16 to 22 kV on the resulting morphology, chemical composition, thickness, crystalline structure, and phase composition of the layers prepared as 10 mm-wide strips on a single stainless-steel substrate. With a low accelerating voltage applied, the best surface homogeneity is obtained. Transfer coefficient values characterizing the elemental transport between the bulk target and the grown layer are evaluated for each constituting element and applied voltage. With the IJD accelerating voltage approaching 22 kV, the coefficients converge upon the values proportional to the atomic number of the element. Such voltage dependence of the IJD elemental transport might be used as a suitable tool for fine-tuning the elemental composition of layers grown from a single deposition target.
Climatic Niche Differentiation between the Invasive Hornet Vespa velutina nigrithorax and Two Native Hornets in Europe, Vespa crabro and Vespa orientalis
Authors: Lioy Simone , Carisio Luca , Manino Aulo , Porporato Marco
Published: March 2023

The introduction and expansion of the Asian yellow-legged hornet (Vespa velutina nigrithorax) in Europe poses concern for multiple reasons, including biodiversity conservation. In addition to the predation of native insects (e.g., bees and wasps), this species may compete with native hornets due to an overlap of their climatic and trophic niches. The aim of this study is to investigate the realised climatic niche of V. v. nigrithorax and its response to climatic conditions and to evaluate the degree of overlap with the niches of the two native Vespa species present in Europe, Vespa crabro and Vespa orientalis. The niches of both native species partially overlap with the niche of the invasive species (Schoener’s D, 0.43 for V. crabro and 0.28 for V. orientalis), although some differences can be detected. V. crabro appears to be more adapted to cold and dry conditions than the invasive species, and V. orientalis is more adapted to arid climates. These differences may provide a competitive advantage to both native species in areas with a lower environmental suitability for V. v. nigrithorax, in the probable event that this species continues to spread, reaching all areas predicted to be suitable in Europe and in the Mediterranean basin.
Training and External Validation of a Predict Nomogram for Type 2 Diabetic Peripheral Neuropathy
Authors: Li Yongsheng , Li Yongnan , Deng Ning , Shi Haonan , Caika Siqingaowa , Sen Gan
Published: March 2023

Background: Diabetic peripheral neuropathy (DPN) is a critical clinical disease with high disability and mortality rates. Early identification and treatment of DPN is critical. Our aim was to train and externally validate a prediction nomogram for early prediction of DPN. Methods: 3012 patients with T2DM were retrospectively studied. These patients were hospitalized between 1 January 2017 and 31 December 2020 in the First Affiliated Hospital of Xinjiang Medical University in Xinjiang, China. A total of 901 patients with T2DM from the Suzhou BenQ Hospital in Jiangsu, China who were hospitalized between 1 January 2019 and 31 December 2020 were considered for external validation. The least absolute shrinkage and selection operator (LASSO) and multivariate logistic regression were performed to identify independent predictors and establish a nomogram to predict the occurrence of DPN. The performance of the nomogram was evaluated using a receiver operating characteristic curve (ROC), a calibration curve, and a decision curve analysis (DCA). Findings: Age, 25-hydroxyvitamin D3 [25(OH)D3], Duration of T2DM, high-density lipoprotein (HDL), hemoglobin A1c (HbA1c), and fasting blood glucose (FBG) were used to establish a nomogram model for predicting the risk of DPN. In the training and validation cohorts, the areas under the curve of the nomogram constructed from the above six factors were 0.8256 (95% CI: 0.8104–0.8408) and 0.8608 (95% CI: 0.8376–0.8840), respectively. The nomogram demonstrated excellent performance in the calibration curve and DCA. Interpretation: This study has developed and externally validated a nomogram model which exhibits good predictive ability in assessing DPN risk among the type 2 diabetes population. It provided clinicians with an accurate and effective tool for the early prediction and timely management of DPN.
Carotid Artery Stenting Using Five-French Distal Radial Vascular Access
Authors: Di Gioia Giuseppe , Salemme Luigi , Ferrone Marco , Cioppa Angelo , Popusoi Grigore , Pucciarelli Armando , Verdoliva Sebastiano , Franzese Michele , Marga Simion , Barbato Emanuele , Tesorio Tullio
Published: March 2023

Carotid artery stenting (CAS) is usually performed through a femoral vascular access using 6–9 Fr guiding catheters. We investigated whether a systematic distal radial approach using 5 Fr guiding sheaths was a safe and effective alternative to transfemoral approach for CAS. From July 2020 to October 2022, two operators at our center systematically performed CAS using a 5 Fr distal radial approach in consecutive patients. The main endpoints of the study were procedural success via distal radial and via proximal or distal radial access. The learning curve was evaluated by comparing the first half of patients versus the second half of patients enrolled. Procedural data and 30-day clinical outcomes were collected. Fifty-one patients were prospectively enrolled. CAS was effectively performed via distal radial access in 45 patients (88%). Overall radial artery success was 92%. Distal radial CAS was successfully performed in 20 out of the first 25 patients enrolled (80%), and in 25 of the last 26 patients enrolled (96%; p = 0.07). Significantly less contrast was administered in the last 26 patients compared to the first 25 enrolled (110 (70, 140) mL vs. 120 (107, 150) mL; p = 0.045). Radial artery occlusion was reported in 1 patient (2%). Only 1 minor stroke (2%) was reported in-hospital and at 30-day follow-up. In conclusion, distal radial CAS using 5 Fr catheters was a safe procedure with a high success rate. The procedure had a relatively short learning curve in operators familiar with transfemoral CAS.
Vascular Extracellular Vesicles Indicate Severe Hepatopulmonary Syndrome in Cirrhosis
Authors: Baweja Sukriti , Kumari Anupama , Negi Preeti , Thangariyal Swati , Subudhi P. Debishree , Gautam Shivani , Mittal Ashmit , Bihari Chhagan
Published: March 2023

Background: Hepatopulmonary syndrome (HPS) is a pulmonary vasculature complication in the setting of liver disease that is characterized by pathological vasodilation resulting in arterial oxygenation defects. We investigated the role of extracellular vesicles (EV) in cirrhosis patients with HPS, as well as the functional effect of EV administration in a common bile duct ligation (CBDL) HPS mouse model. Methods: A total of 113 cirrhosis patients were studied: 42 (Gr. A) with HPS and 71 (Gr. B) without HPS, as well as 22 healthy controls. Plasma levels of EV associated with endothelial cells, epithelial cells, and hepatocytes were measured. The cytokine cargoes were estimated using ELISA. The effect of EV administered intranasally in the CBDL mouse model was investigated for its functional effect in vascular remodeling and inflammation. Results: We found endothelial cells (EC) associated EV (EC-EV) were elevated in cirrhosis patients with and without HPS (p < 0.001) than controls. EC-EV levels were higher in HPS patients (p = 0.004) than in those without HPS. The epithelial cell EVs were significantly high in cirrhosis patients than controls (p < 0.001) but no changes found in patients with HPS than without. There was a progressive increase in EC-EV levels from mild to severe intrapulmonary shunting in HPS patients (p = 0.02 mild vs. severe), and we were able to predict severe HPS with an AUROC of 0.85; p < 0.001. An inverse correlation of EC-EVs was found with hemoglobin (r = −0.24; p = 0.031) and PaO2 (r = 0.690; p = 0.01) and a direct correlation with MELD (r = 0.32; p = 0.014). Further, both TNF-α (p = 0.001) and IL-1β (p = 0.021) as cargo levels were significantly elevated inside the EVs of HPS patients than without HPS. Interestingly, upon administration of intranasal EVs, there was a significant decrease in Evans blue accumulation and lung wet–dry ratio (p = 0.042; 0.038). A significant reduction was also noticed in inflammation and cholestasis. Conclusion: High levels of plasma EC-EV levels were found in patients with HPS with elevated pro-inflammatory cytokine cargoes. EC-EVs were indicative of severe HPS condition. In the CBDL HPS model, we were able to prove the beneficial effects of improving vascular tone, inflammation, and liver pathogenesis.
Universal Causality
Authors: Mahadevan Sridhar
Published: March 2023

Universal Causality is a mathematical framework based on higher-order category theory, which generalizes previous approaches based on directed graphs and regular categories. We present a hierarchical framework called UCLA (Universal Causality Layered Architecture), where at the top-most level, causal interventions are modeled as a higher-order category over simplicial sets and objects. Simplicial sets are contravariant functors from the category of ordinal numbers Δ into sets, and whose morphisms are order-preserving injections and surjections over finite ordered sets. Non-random interventions on causal structures are modeled as face operators that map n-simplices into lower-level simplices. At the second layer, causal models are defined as a category, for example defining the schema of a relational causal model or a symmetric monoidal category representation of DAG models. The third layer corresponds to the data layer in causal inference, where each causal object is mapped functorially into a set of instances using the category of sets and functions between sets. The fourth homotopy layer defines ways of abstractly characterizing causal models in terms of homotopy colimits, defined in terms of the nerve of a category, a functor that converts a causal (category) model into a simplicial object. Each functor between layers is characterized by a universal arrow, which define universal elements and representations through the Yoneda Lemma, and induces a Grothendieck category of elements that enables combining formal causal models with data instances, and is related to the notion of ground graphs in relational causal models. Causal inference between layers is defined as a lifting problem, a commutative diagram whose objects are categories, and whose morphisms are functors that are characterized as different types of fibrations. We illustrate UCLA using a variety of representations, including causal relational models, symmetric monoidal categorical variants of DAG models, and non-graphical representations, such as integer-valued multisets and separoids, and measure-theoretic and topological models.
Model of a Quarter Car Suspension with a Damper Containing Magnetorheological Fluid and with Damaged Parts Controlled by Backstepping Method
Authors: Słomczyński Maciej , Radkowski Stanisław , Makowski Michał
Published: March 2023

The work focuses on the minimization of the body deflection from its equilibrium position after a deflection by force applied to the wheel with the task of simulating obstacles encountered by the wheel. The model presents a quarter of the car’s suspension with a nonlinear spring and a damper with magnetorheological fluid, by which the damping of the suspension is modified. The system was created in harmony with Lyapunov’s stability. The model was designed using Matlab-Simulink. The model was designed for testing many different damaged parts of the suspension, for example, a spring or a damper. In further attempts, the model was tested for numerous damaged parts, and the sequence of events was different. The model was tested for different characteristics of springs and dampers and variable method deflection wheel from its equilibrium position such as force and displacement. This work discusses the detection of damage to the suspension along with the possibility of adapting the MR damper control system to avoid reducing the comfort and safety of the vehicle.
Fast Aero-Structural Model of a Leading-Edge Inflatable Kite
Authors: Cayon Oriol , Gaunaa Mac , Schmehl Roland
Published: March 2023

Soft-wing kites for airborne wind-energy harvesting function as flying tensile membrane structures, each of whose shape depends on the aerodynamic load distribution and vice versa. The strong two-way coupling between shape and loading poses a complex fluid-structure interaction problem. Since computational models for such problems do not yet meet the requirements of being accurate and at the same time fast, kite designers usually work on the basis of intuition and experience, combined with extensive iterative flight testing. This paper presents a fast aero-structural model of leading-edge inflatable kites for the design phase of airborne wind-energy systems. The fluid-structure interaction solver couples two fast and modular models: a particle system model to capture the deformation of the wing and bridle-line system and a 3D nonlinear vortex step method coupled with viscous 2D airfoil polars to describe the aerodynamics. The flow solver was validated with several wing geometries and proved to be accurate and computationally inexpensive for pre-stall angles of attack. The coupled aero-structural model was validated using experimental data, showing good agreement in the deformations and aerodynamic forces. Therefore, the speed and accuracy of this model make it an excellent foundation for a kite design tool.
Balancing the Active Power of a Railway Traction Power Substation with an sp-RPC
Authors: Barros Luis , Martins António , Pinto José
Published: March 2023

The railway system is one of the safest, most efficient, and environmentally friendly means of land transport for people and goods. However, as the demand for mobility has increased, the current railway system has shown some weaknesses, requiring an increase in catenary power in order to be able to supply power to longer trains and faster locomotives, as well as to increase rail traffic. This paper proposes a control algorithm to be implemented in a sectioning post-Rail Power Conditioner (sp-RPC). The sp-RPC is connected to the neutral section between two traction power substations (TPS). With the control algorithm, it is possible to minimize the existing unbalance of the active powers of each TPS. In a regenerative braking condition, this surplus energy can be used to assist the traction of another locomotive on the existing overhead line. In this way, it is possible to increase the capacity of the overhead line. The analysis was performed with computer models using a modular multilevel converter (MMC) topology for the sp-RPC. Quantitative results for different consumption events of the locomotives and the analysis of the response to these variations are presented.
A Scientometric Review of Environmental Valuation Research with an Altmetric Pathway for the Future
Authors: Olukolajo Michael Ayodele , Oyetunji Abiodun Kolawole , Amaechi Chiemela Victor
Published: March 2023

Environmental valuation (EV) research has advanced significantly as a method of assigning value to environmental goods, many of which lack readily discernible market values. The term “environmental valuation” describes a number of methods for putting monetary values on environmental effects, particularly non-market effects. Over the years, there has been a continuous increase in the number of publications on the topic. According to searches via the Scopus and Web of Science (WoS) databases, the phrase “environmental valuation” first occurred in 1987. The current research examines patterns in publishing rates over the previous three decades to analyse environmental valuation activity. To identify the future pathway, a research pattern was identified using Scopus, WoS, and the Altmetric Explorer. The data were postprocessed using VOSviewer to identify the mapping networks; then, Voyant Tools were used to explore the keywords. A summary of the demand for environmental valuation is also provided based on the literature review. However, the findings of this historical analysis indicate that despite the academic efforts on this subject, environmental valuation is not used in research as much as one may anticipate. In addition, the study gives a general outline of the future directions that environmental valuation research is anticipated to follow in light of the current academic research initiatives as well as academic market and policy market research efforts. The study shows that the United Kingdom (UK) showed the highest publications by location in this field. Additionally, the study shows that the choice experiment approach is preferred over the contingent valuation method, and this work illustrates this preference. It also demonstrates that only a small number of papers have had a significant influence on the researchers in this field.
Screening and Application of Novel Homofermentative Lactic Acid Bacteria Results in Low-FODMAP Whole-Wheat Bread
Authors: Borowska Małgorzata , Ispiryan Lilit , Neylon Emma , Sahin Aylin W. , Murphy Craig P. , Zannini Emanuele , Arendt Elke K. , Coffey Aidan
Published: March 2023

FODMAPs are fermentable oligo-, di-, monosaccharides, and polyols. The application of homofermentative lactic acid bacteria (LAB) has been investigated as a promising approach for producing low-FODMAP whole-wheat bread. The low-FODMAP diet is recommended to treat irritable bowel syndrome (IBS). Wheat flour is staple to many diets and is a significant source of fructans, which are considered FODMAPs. The reduction of fructans via sourdough fermentation, generally associated with heterofermentative lactic acid bacteria (LAB), often leads to the accumulation of other FODMAPs. A collection of 244 wild-type LAB strains was isolated from different environments and their specific FODMAP utilisation profiles established. Three homofermentative strains were selected for production of whole-wheat sourdough bread. These were Lactiplantibacillus plantarum FST1.7 (FST1.7), Lacticaseibacillus paracasei R3 (R3), and Pediococcus pentosaceus RYE106 (RYE106). Carbohydrate levels in flour, sourdoughs (before and after 48 h fermentation), and resulting breads were analysed via HPAEC-PAD and compared with whole-wheat bread leavened with baker’s yeast. While strain R3 was the most efficient in FODMAP reduction, breads produced with all three test strains had FODMAP content below cut-off levels that would trigger IBS symptoms. Results of this study highlighted the potential of homofermentative LAB in producing low-FODMAP whole-wheat bread.
Tissue Bioconcentration Pattern and Biotransformation of Per-Fluorooctanoic Acid (PFOA) in Cyprinus carpio (European Carp)—An Extensive In Vivo Study
Authors: Petre Valentina Andreea , Chiriac Florentina Laura , Lucaciu Irina Eugenia , Paun Iuliana , Pirvu Florinela , Iancu Vasile Ion , Novac Laura , Gheorghe Stefania
Published: March 2023

The perfluoroalkyl substances (PFAS) represent a persistent class of synthetic chemicals that spread in the environment as a result of industrialization. Due to their bioaccumulative and endocrine disruption implications, these chemicals can affect food quality and human health, respectively. In the present study, the bioconcentration and biotransformation of perfluorooctanoic acid (PFOA) in common carp (Cyprinus carpio) were evaluated in a biphasic system (exposure and depuration). Carp were continuously exposed, under laboratory conditions, to 10 (Experiment 1) and 100 (Experiment 2) µg/L PFOA for 14 weeks, followed by a wash out period of 3 weeks. Fish organs and tissues were collected at 8, 12, 14 weeks of exposure and at week 17, after the depuration period. The results obtained from the LC-MS/MS analysis showed the presence of PFOA in all studied organs. The highest values of PFOA were identified in the gallbladder (up to 2572 ng/g d.w.) in Experiment 1 and in the gallbladder (up to 18,640 ng/g d.w.) and kidneys (up to 13,581 ng/g d.w.) in Experiment 2. The average BCF varied between 13.4 and 158 L/Kg in Experiment 1 and between 5.97 and 80.3 L/Kg in Experiment 2. Four biotransformation products were identified and quantified in all organs, namely: PFBA, PFPeA, PFHxA, and PFHpA. PFBA was proven to be the dominant biotransformation product, with the highest values being determined after 8 weeks of exposure in the kidney, gallbladder, brain, liver, and gonads in both experiments. Because freshwater fish are an important food resource for the human diet, the present study showed the fishes’ capacity to accumulate perfluoroalkyl substances and their metabolites. The study revealed the necessity of monitoring and risk studies of new and modern synthetic chemicals in aquatic resources.
Performance of an Expert Sensory Panel and Instrumental Measures for Assessing Eating Fruit Quality Attributes in a Pear Breeding Programme
Authors: Lozano Lidia , Iglesias Ignasi , Puy Jaume , Echeverria Gemma
Published: March 2023

Breeding programmes count on stable trained panels that support breeding evaluation selections. This work aimed to evaluate the performance of a small expert panel in the join IRTA-PFR breeding programme to validate its use in the sensory assessments of fruit pear genotypes during the selection process. A breeding F1 population of 80 pear seedlings from this programme was used. Descriptors and standard references used for sensory evaluations of pear attributes were previously defined by the four members of the expert panel. A General Procrustes Analysis (GPA) was applied to analyse the relations between instrumental and sensory traits. The results showed a good relationship between sensory attributes such as firmness and crispness with penetrometer measures. A high correlation was also found between sensory sourness and titratable acidity (TA). Panel performance was evaluated in terms of reproducibility, homogeneity, and panel consonance. The results indicated that the experts were very consistent and had a good performance. The work demonstrates, for the first time, that a small expert trained panel could be efficiently used in pear breeding programmes and allows for the selection process in a more economical and available way in contrast to the larger sensory panels conventionally used.
Alleviation of Alcoholic Fatty Liver by Dendrobium officinale Flower Extracts due to Regulation of Gut Microbiota and Short-Chain Fatty Acids in Mice Exposed to Chronic Alcohol
Authors: Zhang Jingchi , Fan Jiakun , Luo Hui , Liang Zhengwei , Guan Yanhui , Lei Xin , Bo Nianguo , Zhao Ming
Published: March 2023

Alcoholic fatty liver disease (AFLD) is caused by long-term heavy alcohol consumption; therefore, useful and practical methods for the prevention of AFLD are urgently needed. The edible flower of Dendrobium officinale contains diverse flavonoids, and has shown antioxidant activity as well as antihypertensive and anti-inflammatory effects. In this study, an AFLD model was established, the protective effect of D. officinale flower (DOF) ethanol extract on AFLD was evaluated, and its mechanisms were investigated by analyzing gut microbiota and short-chain fatty acids (SCFAs). DOF extract (DOFE) supplementation promoted alcohol metabolism, restored hepatic antioxidant capacity, alleviated oxidative stress, reduced inflammatory factor levels, and inhibited dyslipidemia induced by alcohol intake in chronic alcohol-exposed mice, especially in the high DOFE group. Moreover, DOFE supplementation increased the diversity, structure, and composition of the gut microbiota in mice, restored some of the abnormal SCFA levels caused by AFLD, and helped restore intestinal function. DOFE supplementation significantly increased the relative abundance of Akkermansia, suggesting that Akkermansia may be a potential target of the protective effect of DOFE. Therefore, DOFE supplementation to improve the composition of the gut microbiota may be an effective therapeutic strategy for the prevention of AFLD.
Synergistic Effect of Combination of Various Microbial Hurdles in the Biopreservation of Meat and Meat Products—Systematic Review
Authors: Karbowiak Marcelina , Szymański Piotr , Zielińska Dorota
Published: March 2023

The control of spoilage microorganisms and foodborne pathogens in meat and meat products is a challenge for food producers, which potentially can be overcome through the combined use of biopreservatives, in the form of a mix of various microbial hurdles. The objective of this work is to systematically review the available knowledge to reveal whether various microbial hurdles applied in combination can pose an effective decontamination strategy for meat and meat products. PubMed, Web of Science, and Scopus were utilized to identify and evaluate studies through February 2023. Search results yielded 45 articles that met the inclusion criteria. The most common meat biopreservatives were combinations of various starter cultures (24 studies), and the use of mixtures of non-starter protective cultures (13 studies). In addition, studies evaluating antimicrobial combinations of bacteriocins with other bacteriocins, BLIS (bacteriocin-like inhibitory substance), non-starter protective cultures, reuterin, and S-layer protein were included in the review (7 studies). In one study, a biopreservative mixture comprised antifungal protein PgAFP and protective cultures. The literature search revealed a positive effect, in most of the included studies, of the combination of various bacterial antimicrobials in inhibiting the growth of pathogenic and spoilage bacteria in meat products. The main advantages of the synergistic effect achieved were: (1) the induction of a stronger antimicrobial effect, (2) the extension of the spectrum of antibacterial action, and (3) the prevention of the regrowth of undesirable microorganisms. Although further research is required in this area, the combination of various microbial hurdles can pose a green and valuable biopreservation approach for maintaining the safety and quality of meat products.
The Fluorescence Response of Four Crystalline Starches According to Ultrasound-Assisted Starch-Salicylic Acid Inclusions
Authors: Pei Rui , Lu Hao , Wang Fan , Ma Rongrong , Tian Yaoqi
Published: March 2023

Fluorescence has shown its superior performance in the fields of starch physicochemical properties, starch–based materials, and the interactions of starch with small molecules. However, it has not been well explored in the fluorescence characteristics of starch. Herein, the fluorescence properties of four crystalline starches (A–type tapioca starch, B–type potato starch, C–type pea starch, and V–type starch, prepared with corn starch and stearic acid) were investigated using salicylic acid (SA) as an indicator. The results of inverted fluorescence microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis indicated that SA could be included by starch. X–ray diffraction analysis further demonstrated that the inclusion of SA did not change the crystalline of the four crystal types of starches, which could provide a prerequisite for comparing the different fluorescence properties of the four crystal types of starches. Fluorescence enhancements of the four inclusions were 264.5 (B–type), 206 (C–type), 51.2 (V–type), and 28 (A–type). These results provide new insights for analyzing the fluorescence response of starch.
High Internal Phase Emulsions Stabilized by Pea Protein Isolate Modified by Ultrasound Combined with pH-Shifting: Micromorphology, Rheology, and Physical Stability
Authors: Zhang Jingnan , Zhao Siqi , Li Linte , Kong Baohua , Liu Haotian
Published: March 2023

In this study, the interfacial behavior of high internal phase emulsions (HIPEs), stabilized by ultrasound combined with pH-shifting modified pea protein isolate (MPPI), was investigated, and its emulsification process and stabilization mechanism were discussed. The effects of MPPI concentration on the micromorphology, droplet size, rheology, and stability of HIPEs were investigated. As the MPPI concentration increased, the appearance of HIPEs gradually changed from a relatively fluid state to a plastic solid-like state with detailed texture. There occurred a gradual decrease in droplet size, the cohering of an orderly and tight arrangement, in addition to the formation of a bilayer elastic interface layer. The macro- and microrheological assessments confirmed that the apparent viscosity, storage modulus, elasticity index, and macroscopic viscosity index increased gradually. Furthermore, it was demonstrated that 5 wt% MPPI-stabilized HIPEs had the potential to be used as 3D printing inks. Stability evaluation showed that the TURBISCAN stability index decreased and centrifugal stability increased. The appearance and microstructure remained highly stable after heating at 80 °C for 30 min and storage at 4 ℃ for 90 days. These findings confirm that MPPI improves the rheological behavior and stability of HIPEs by modulating the interfacial adsorption and network structure.
School Parent Attitudes and Perceptions Relating to Animals, Animal-Assisted Interventions, and the Support of Children’s Mental Health
Authors: Leos Rhoda A. , Cuccaro Paula M. , Herbold John R. , Hernandez Belinda F.
Published: March 2023

Growing awareness of the negative effects of trauma has led San Antonio, TX, school districts to expand efforts that can help mitigate these effects and support mental health. Given the literature around the psychological benefits of human–animal interactions, the concept of incorporating animals in treatments or interventions is not a new one. While schools have begun considering or utilizing animal-assisted interventions (AAIs), there have been limited efforts to understand existing perceptions relating to animals and AAIs among school parents in this Hispanic community. To address this gap, a cross-sectional study consisting of a 34-item survey was conducted to explore attitudes, knowledge, and perceptions relating to animals (i.e., pets), AAIs, and the need for supporting young children’s mental health among parents. A total of 187 surveys from two school districts were completed and utilized for analysis. The study’s findings demonstrate that parents acknowledged the importance of addressing mental health issues early on and were aware of the health benefits human–animal interactions can provide. Furthermore, parents had positive attitudes toward pets and positive perceptions toward AAIs in schools. Some implementation concerns were expressed relating to safety and well-being. Overall, these findings suggest there is existing parent support in using AAIs as a trauma-informed strategy and school innovation.
Social Experiences, Discrimination, and Violence among Men Who Have Sex with Men in a Northern Brazilian Capital
Authors: Carneiro Alexandre Mansuê Ferreira , Rodrigues Yan Corrêa , Dolabela Maria Fani , Lima Luana Nepomuceno Godim Costa , Guimarães Ricardo José de Paula Souza , Kendall Carl , Kerr Ligia Regina Franco Sansigolo , Lima Karla Valéria Batista
Published: March 2023

Men who have sex with men who suffer stigmatization and discrimination become more fragile in facing life’s problems, such as the search for treatment in health services. In the present study, the social aspects related to discrimination and violence among men who have sex with men in Belém, Pará, are evaluated. Data were obtained by applying the respondent-driven sampling method to recruit 349 participants aged 18 years or older and who reported having had at least one sexual relationship with a man in the last 12 months. Data were collected from June to December 2016 in a semi-structured interview. Five seeds were initially recruited who applied RDS. The vast majority were between 18 and 35 years old, had completed elementary school but not high school, and were of mixed race. Almost a third lived in peripheral neighborhoods and were employed/self-employed. Additionally, most participants reported having suffered aggression/discrimination, more often in religious contexts, with family or in health services. The findings reported here may contribute to the development of public policies aimed at this population and indicate the need for new strategies to combat sexually transmitted infections, stigma, and discrimination suffered by this population.
Valuing Exchange Options under an Ornstein-Uhlenbeck Covariance Model
Authors: Villamor Enrique , Olivares Pablo
Published: March 2023

In this paper we study the pricing of exchange options between two underlying assets whose dynamic show a stochastic correlation with random jumps. In particular, we consider a Ornstein-Uhlenbeck covariance model, with Levy Background Noise Processes driven by Inverse Gaussian subordinators. We use expansions in terms of Taylor polynomials and cubic splines to approximately compute the price of the derivative contract. Our findings show that the later approach provides an efficient way to compute the price when compared with a Monte Carlo method, while maintaining an equivalent degree of accuracy.
Comparison of SPEED, S-Trap, and In-Solution-Based Sample Preparation Methods for Mass Spectrometry in Kidney Tissue and Plasma
Authors: Templeton Evelyn M. , Pilbrow Anna P. , Kleffmann Torsten , Pickering John W. , Rademaker Miriam T. , Scott Nicola J. A. , Ellmers Leigh J. , Charles Christopher J. , Endre Zoltan H. , Richards A. Mark , Cameron Vicky A. , Lassé Moritz
Published: March 2023

Mass spectrometry is a powerful technique for investigating renal pathologies and identifying biomarkers, and efficient protein extraction from kidney tissue is essential for bottom-up proteomic analyses. Detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-coupled mass spectrometry, requiring additional purification and buffer-exchange steps. This study compares two well-established detergent-based methods for protein extraction (in-solution sodium deoxycholate (SDC); suspension trapping (S-Trap)) with the recently developed sample preparation by easy extraction and digestion (SPEED) method, which uses strong acid for denaturation. We compared the quantitative performance of each method using label-free mass spectrometry in both sheep kidney cortical tissue and plasma. In kidney tissue, SPEED quantified the most unique proteins (SPEED 1250; S-Trap 1202; SDC 1197). In plasma, S-Trap produced the most unique protein quantifications (S-Trap 150; SDC 148; SPEED 137). Protein quantifications were reproducible across biological replicates in both tissue (R2 = 0.85–0.90) and plasma (SPEED R2 = 0.84; SDC R2 = 0.76, S-Trap R2 = 0.65). Our data suggest SPEED as the optimal method for proteomic preparation in kidney tissue and S-Trap or SPEED as the optimal method for plasma, depending on whether a higher number of protein quantifications or greater reproducibility is desired.
Temperature Effect on the Corrosion Inhibition of Carbon Steel by Polymeric Ionic Liquids in Acid Medium
Authors: Gómez-Sánchez Giselle , Olivares-Xometl Octavio , Arellanes-Lozada Paulina , Likhanova Natalya V. , Lijanova Irina V. , Arriola-Morales Janette , Díaz-Jiménez Víctor , López-Rodríguez Josué
Published: March 2023

In the present research work, the temperature effect on the corrosion inhibition process of API 5L X60 steel in 1 M H2SO4 by employing three vinylimidazolium poly(ionic liquid)s (PILs) was studied by means of electrochemical techniques, surface analysis and computational simulation. The results revealed that the maximal inhibition efficiency (75%) was achieved by Poly[VIMC4][Im] at 308 K and 175 ppm. The PILs showed Ecorr displacements with respect to the blank from −14 mV to −31 mV, which revealed the behavior of mixed-type corrosion inhibitors (CIs). The steel micrographs, in the presence and absence of PILs, showed less surface damage in the presence of PILs, thus confirming their inhibiting effect. The computational studies of the molecular orbitals and molecular electrostatic potential of the monomers suggested that the formation of a protecting film could be mainly due to the nitrogen and oxygen heteroatoms present in each structure.
Benefits of Biotics for Cardiovascular Diseases
Authors: Hijová Emília
Published: March 2023

Cardiovascular diseases are the main cause of death in many countries, and the better prevention and prediction of these diseases would be of great importance for individuals and society. Nutrition, the gut microbiota, and metabolism have raised much interest in the field of cardiovascular disease research in the search for the main mechanisms that promote cardiovascular diseases. Understanding the interactions between dietary nutrient intake and the gut microbiota-mediated metabolism may provide clinical insight in order to identify individuals at risk of cardiometabolic disease progression, as well as other potential therapeutic targets to mitigate the risk of cardiometabolic disease progression. The development of cardiometabolic diseases can be modulated by specific beneficial metabolites derived from bacteria. Therefore, it is very important to investigate the impact of these metabolites on human health and the possibilities of modulating their production with dietary supplements called biotics.
Lanthanides as Calcium Mimetic Species in Calcium-Signaling/Buffering Proteins: The Effect of Lanthanide Type on the Ca2+/Ln3+ Competition
Authors: Nikolova Valya , Kircheva Nikoleta , Dobrev Stefan , Angelova Silvia , Dudev Todor
Published: March 2023

Lanthanides, the 14 4f-block elements plus Lanthanum, have been extensively used to study the structure and biochemical properties of metalloproteins. The characteristics of lanthanides within the lanthanide series are similar, but not identical. The present research offers a systematic investigation of the ability of the entire Ln3+ series to substitute for Ca2+ in biological systems. A well-calibrated DFT/PCM protocol is employed in studying the factors that control the metal selectivity in biological systems by modeling typical calcium signaling/buffering binding sites and elucidating the thermodynamic outcome of the competition between the “alien” La3+/Ln3+ and “native” Ca2+, and La3+ − Ln3+ within the lanthanide series. The calculations performed reveal that the major determinant of the Ca2+/Ln3+ selectivity in calcium proteins is the net charge of the calcium binding pocket; the more negative the charge, the higher the competitiveness of the trivalent Ln3+ with respect to its Ca2+ contender. Solvent exposure of the binding site also influences the process; buried active centers with net charge of −4 or −3 are characterized by higher Ln3+ over Ca2+ selectivity, whereas it is the opposite for sites with overall charge of −1. Within the series, the competition between La3+ and its fellow lanthanides is determined by the balance between two competing effects: electronic (favoring heavier lanthanides) and solvation (generally favoring the lighter lanthanides).
Quantum Mechanical Assessment of Protein–Ligand Hydrogen Bond Strength Patterns: Insights from Semiempirical Tight-Binding and Local Vibrational Mode Theory
Authors: Madushanka Ayesh , Moura Renaldo T. , Verma Niraj , Kraka Elfi
Published: March 2023

Hydrogen bonds (HB)s are the most abundant motifs in biological systems. They play a key role in determining protein–ligand binding affinity and selectivity. We designed two pharmaceutically beneficial HB databases, database A including ca. 12,000 protein–ligand complexes with ca. 22,000 HBs and their geometries, and database B including ca. 400 protein–ligand complexes with ca. 2200 HBs, their geometries, and bond strengths determined via our local vibrational mode analysis. We identified seven major HB patterns, which can be utilized as a de novo QSAR model to predict the binding affinity for a specific protein–ligand complex. Glycine was reported as the most abundant amino acid residue in both donor and acceptor profiles, and N–H⋯O was the most frequent HB type found in database A. HBs were preferred to be in the linear range, and linear HBs were identified as the strongest. HBs with HB angles in the range of 100–110°, typically forming intramolecular five-membered ring structures, showed good hydrophobic properties and membrane permeability. Utilizing database B, we found a generalized Badger’s relationship for more than 2200 protein–ligand HBs. In addition, the strength and occurrence maps between each amino acid residue and ligand functional groups open an attractive possibility for a novel drug-design approach and for determining drug selectivity and affinity, and they can also serve as an important tool for the hit-to-lead process.
Flavonoids as Promising Natural Compounds in the Prevention and Treatment of Selected Skin Diseases
Authors: Čižmárová Beáta , Hubková Beáta , Tomečková Vladimíra , Birková Anna
Published: March 2023

Phytochemicals represent a large and diverse group of naturally occurring compounds, bioactive nutrients, or phytonutrients produced by plants, widely found in fruits, vegetables, whole grains products, legumes, beans, herbs, seeds, nuts, tea, and dark chocolate. They are classified according to their chemical structures and functional properties. Flavonoids belong to the phenolic class of phytochemicals with potential solid pharmacological effects as modulators of multiple signal transduction pathways. Their beneficial effect on the human body is associated with their antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic properties. Flavonoids are also widely used in various nutritional, pharmaceutical, medical, and cosmetic applications. In our review, we discuss the positive effect of flavonoids on chronic skin diseases such as vitiligo, psoriasis, acne, and atopic dermatitis.
Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos
Authors: Yan Wenhua , Zhang Tian , Li Shuaiting , Wang Yunpeng , Zhu Li , Cao Yu , Lai Xiaofang , Huang Huizhe
Published: March 2023

Areca nut has been listed as one of the most addictive substances, along with tobacco, alcohol and caffeine. Areca nut contains seven psychoactive alkaloids; however, the effects of these alkaloids on embryonic development and motor behavior are rarely addressed in zebrafish embryo-larvae. Herein, we investigated the effects of exposure to three alkaloids (arecoline and secondary metabolites—arecaidine and arecoline N-oxide) on the developmental parameters, locomotive behavior, oxidative stress and transcriptome of zebrafish embryos. Zebrafish embryos exposed to different concentrations (0, 0.1, 1, 10, 100 and 1000 μM) of arecoline, arecaidine and arecoline N-oxide showed no changes in mortality and hatchability rates, but the malformation rate of zebrafish larvae was significantly increased in a dose-dependent manner and accompanied by changes in body length. Moreover, the swimming activity of zebrafish larvae decreased, which may be due to the increase in reactive oxygen species and the imbalance between oxidation and antioxidation. Meanwhile, transcriptome analysis showed that endoplasmic reticulum stress and the apoptosis p53 signaling pathway were significantly enriched after exposure to arecoline and arecoline N-oxide. However, arecaidine exposure focuses on protein synthesis and transport. These findings provide an important reference for risk assessment and early warning of areca nut alkaloid exposure.