bluuebunny/crossref_metadata_2025 huggingface dataset:
Parental Preconception and Pre-Hatch Exposure to A Developmental Insult Alters Offspring’s Gene Expression and Epigenetic Regulations: An Avian Model
Authors: Rimawi Issam , Turgeman Gadi , Avital-Cohen Nataly , Rozenboim Israel , Yanai Joseph
Published: March 2023

Parental exposure to insults was initially considered safe if stopped before conception. In the present investigation, paternal or maternal preconception exposure to the neuroteratogen chlorpyrifos was investigated in a well-controlled avian model (Fayoumi) and compared to pre-hatch exposure focusing on molecular alterations. The investigation included the analysis of several neurogenesis, neurotransmission, epigenetic and microRNA genes. A significant decrease in the vesicular acetylcholine transporter (SLC18A3) expression was detected in the female offspring in the three investigated models: paternal (57.7%, p < 0.05), maternal (36%, p < 0.05) and pre-hatch (35.6%, p < 0.05). Paternal exposure to chlorpyrifos also led to a significant increase in brain-derived neurotrophic factor (BDNF) gene expression mainly in the female offspring (27.6%, p < 0.005), while its targeting microRNA, miR-10a, was similarly decreased in both female (50.5%, p < 0.05) and male (56%, p < 0.05) offspring. Doublecortin’s (DCX) targeting microRNA, miR-29a, was decreased in the offspring after maternal preconception exposure to chlorpyrifos (39.8%, p < 0.05). Finally, pre-hatch exposure to chlorpyrifos led to a significant increase in protein kinase C beta (PKCß; 44.1%, p < 0.05), methyl-CpG-binding domain protein 2 (MBD2; 44%, p < 0.01) and 3 (MBD3; 33%, p < 0.05) genes expression in the offspring. Although extensive studies are required to establish a mechanism–phenotype relationship, it should be noted that the current investigation does not include phenotype assessment in the offspring.
Gene Expression Analysis of Immune Regulatory Genes in Circulating Tumour Cells and Peripheral Blood Mononuclear Cells in Patients with Colorectal Carcinoma
Authors: Aktar Sharmin , Hamid Faysal Bin , Gamage Sujani , Cheng Tracie , Pakneshan Nahal , Lu Cu , Islam Farhadul , Gopalan Vinod , Lam Alfred
Published: March 2023

Information regarding genetic alterations of driver cancer genes in circulating tumour cells (CTCs) and their surrounding immune microenvironment nowadays can be employed as a real-time monitoring platform for translational applications such as patient response to therapeutic targets, including immunotherapy. This study aimed to investigate the expression profiling of these genes along with immunotherapeutic target molecules in CTCs and peripheral blood mononuclear cells (PBMCs) in patients with colorectal carcinoma (CRC). Expression of p53, APC, KRAS, c-Myc, and immunotherapeutic target molecules PD-L1, CTLA-4, and CD47 in CTCs and PBMCs were analysed by qPCR. Their expression in high versus low CTC-positive patients with CRC was compared and clinicopathological correlations between these patient groups were analysed. CTCs were detected in 61% (38 of 62) of patients with CRC. The presence of higher numbers of CTCs was significantly correlated with advanced cancer stages (p = 0.045) and the subtypes of adenocarcinoma (conventional vs. mucinous, p = 0.019), while being weakly correlated with tumour size (p = 0.051). Patients with lower numbers of CTCs had higher expression of KRAS. Higher KRAS expression in CTCs was negatively correlated with tumour perforation (p = 0.029), lymph node status (p = 0.037), distant metastasis (p = 0.046) and overall staging (p = 0.004). CTLA-4 was highly expressed in both CTCs and PBMCs. In addition, CTLA-4 expression was positively correlated with KRAS (r = 0.6878, p = 0.002) in the enriched CTC fraction. Dysregulation of KRAS in CTCs might evade the immune system by altering the expression of CTLA-4, providing new insights into the selection of therapeutic targets at the onset of the disease. Monitoring CTCs counts, as well as gene expression profiling of PBMCs, can be helpful in predicting tumour progression, patient outcome and treatment.
The CCCH-Type Zinc-Finger Protein GhC3H20 Enhances Salt Stress Tolerance in Arabidopsis thaliana and Cotton through ABA Signal Transduction Pathway
Authors: Zhang Qi , Zhang Jingjing , Wei Fei , Fu Xiaokang , Wei Hengling , Lu Jianhua , Ma Liang , Wang Hantao
Published: March 2023

The CCCH zinc-finger protein contains a typical C3H-type motif widely existing in plants, and it plays an important role in plant growth, development, and stress responses. In this study, a CCCH zinc-finger gene, GhC3H20, was isolated and thoroughly characterized to regulate salt stress in cotton and Arabidopsis. The expression of GhC3H20 was up-regulated under salt, drought, and ABA treatments. GUS activity was detected in the root, stem, leaves, and flowers of ProGhC3H20::GUS transgenic Arabidopsis. Compared with the control, the GUS activity of ProGhC3H20::GUS transgenic Arabidopsis seedlings under NaCl treatment was stronger. Through the genetic transformation of Arabidopsis, three transgenic lines of 35S-GhC3H20 were obtained. Under NaCl and mannitol treatments, the roots of the transgenic lines were significantly longer than those of the wild-type (WT) Arabidopsis. The leaves of the WT turned yellow and wilted under high-concentration salt treatment at the seedling stage, while the leaves of the transgenic Arabidopsis lines did not. Further investigation showed that compared with the WT, the content of catalase (CAT) in the leaves of the transgenic lines was significantly higher. Therefore, compared with the WT, overexpression of GhC3H20 enhanced the salt stress tolerance of transgenic Arabidopsis. A virus-induced gene silencing (VIGS) experiment showed that compared with the control, the leaves of pYL156-GhC3H20 plants were wilted and dehydrated. The content of chlorophyll in pYL156-GhC3H20 leaves was significantly lower than those of the control. Therefore, silencing of GhC3H20 reduced salt stress tolerance in cotton. Two interacting proteins (GhPP2CA and GhHAB1) of GhC3H20 have been identified through a yeast two-hybrid assay. The expression levels of PP2CA and HAB1 in transgenic Arabidopsis were higher than those in the WT, and pYL156-GhC3H20 had expression levels lower than those in the control. GhPP2CA and GhHAB1 are the key genes involved in the ABA signaling pathway. Taken together, our findings demonstrate that GhC3H20 may interact with GhPP2CA and GhHAB1 to participate in the ABA signaling pathway to enhance salt stress tolerance in cotton.
Ginsenoside Rb1 Improves Post-Cardiac Arrest Myocardial Stunning and Cerebral Outcomes by Regulating the Keap1/Nrf2 Pathway
Authors: Chen Long , Geng Na , Chen Taiwei , Xiao Qingqing , Zhang Hengyuan , Huo Huanhuan , Jiang Lisheng , Shao Qin , He Ben
Published: March 2023

The prognosis of cardiac arrest (CA) is dismal despite the ongoing progress in cardiopulmonary resuscitation (CPR). ginsenoside Rb1 (Gn-Rb1) has been verified to be cardioprotective in cardiac remodeling and cardiac ischemia/reperfusion (I/R) injury, but its role is less known in CA. After 15 min of potassium chloride-induced CA, male C57BL/6 mice were resuscitated. Gn-Rb1 was blindly randomized to mice after 20 s of CPR. We assessed the cardiac systolic function before CA and 3 h after CPR. Mortality rates, neurological outcome, mitochondrial homeostasis, and the levels of oxidative stress were evaluated. We found that Gn-Rb1 improved the long-term survival during the post-resuscitation period but did not affect the ROSC rate. Further mechanistic investigations revealed that Gn-Rb1 ameliorated CA/CPR-induced mitochondrial destabilization and oxidative stress, partially via the activation of Keap1/Nrf2 axis. Gn-Rb1 improved the neurological outcome after resuscitation partially by balancing the oxidative stress and suppressing apoptosis. In sum, Gn-Rb1 protects against post-CA myocardial stunning and cerebral outcomes via the induction of the Nrf2 signaling pathway, which may offer a new insight into therapeutic strategies for CA.
Molecular Pathology, Diagnostics and Therapeutics: A Story of Success in 2022
Authors: Bustin Stephen A.
Published: March 2023

Molecular pathology, diagnostics and therapeutics are three closely related topics of critical importance in medical research and clinical practice [...]
Study on Removal Mechanism for Copper Cyanide Complex Ions in Water: Ion Species Differences and Evolution Process
Authors: Liu Ying , Sun Baogang , Jia Wenting , Wang Yuan , Huang Lijia , Ning Pengge , Yuan Shaojun
Published: March 2023

A large amount of cyanide-containing wastewater is discharged during electrode material synthesis. Among them, cyanides will form metal–cyanide complex ions which possess high stability, making it challenging to separate them from these wastewaters. Therefore, it is imperative to understand the complexation mechanism of cyanide ions and heavy metal ions from wastewater in order to obtain a deep insight into the process of cyanide removal. This study employs Density Functional Theory (DFT) calculations to reveal the complexation mechanism of metal–cyanide complex ions formed by the interaction of Cu+ and CN− in copper cyanide systems and its transformation patterns. Quantum chemical calculations show that the precipitation properties of Cu(CN)43− can assist in the removal of CN−. Therefore, transferring other metal–cyanide complex ions to Cu(CN)43− can achieve deep removal. OLI studio 11.0 analyzed the optimal process parameters of Cu(CN)43− under different conditions and determined the optimal process parameters of the removal depth of CN−. This work has the potential to contribute to the future preparation of related materials such as CN− removal adsorbents and catalysts and provide theoretical foundations for the development of more efficient, stable, and environmentally friendly next-generation energy storage electrode materials.
The Role of Citicoline and Coenzyme Q10 in Retinal Pathology
Authors: García-López Claudia , García-López Verónica , Matamoros José A. , Fernández-Albarral José A. , Salobrar-García Elena , de Hoz Rosa , López-Cuenca Inés , Sánchez-Puebla Lidia , Ramírez José M. , Ramírez Ana I. , Salazar Juan J.
Published: March 2023

Ocular neurodegenerative diseases such as glaucoma, diabetic retinopathy, and age-related macular degeneration are common retinal diseases responsible for most of the blindness causes in the working-age and elderly populations in developed countries. Many of the current treatments used in these pathologies fail to stop or slow the progression of the disease. Therefore, other types of treatments with neuroprotective characteristics may be necessary to allow a more satisfactory management of the disease. Citicoline and coenzyme Q10 are molecules that have neuroprotective, antioxidant, and anti-inflammatory properties, and their use could have a beneficial effect in ocular neurodegenerative pathologies. This review provides a compilation, mainly from the last 10 years, of the main studies that have been published on the use of these drugs in these neurodegenerative diseases of the retina, analyzing the usefulness of these drugs in these pathologies.
Fisetin, a Natural Polyphenol, Ameliorates Endometriosis Modulating Mast Cells Derived NLRP-3 Inflammasome Pathway and Oxidative Stress
Authors: Arangia Alessia , Marino Ylenia , Fusco Roberta , Siracusa Rosalba , Cordaro Marika , D’Amico Ramona , Macrì Francesco , Raffone Emanuela , Impellizzeri Daniela , Cuzzocrea Salvatore , Di Paola Rosanna
Published: March 2023

A chronic, painful, and inflammatory condition known as endometriosis is defined by the extra-uterine development of endometrial tissue. The aim of this study was to evaluate the beneficial effects of fisetin, a naturally occurring polyphenol that is frequently present in a variety of fruits and vegetables. Uterine fragments were injected intraperitoneally to cause endometriosis, and fisetin was given orally every day. At 14 days of treatment, laparotomy was performed, and the endometrial implants and peritoneal fluids were collected for histological, biochemical, and molecular analyses. Rats subjected to endometriosis presented important macroscopic and microscopic changes, increased mast cell (MC) infiltration, and fibrosis. Fisetin treatment reduced endometriotic implant area, diameter, and volumes, as well as histological alterations, neutrophil infiltration, cytokines release, the number of MCs together with the expression of chymase and tryptase, and diminished α smooth muscle actin (α-sma) and transforming growth factor beta (TGF β) expressions. In addition, fisetin was able to reduce markers of oxidative stress as well as nitrotyrosine and Poly ADP ribose expressions and increase apoptosis in endometrial lesions. In conclusion, fisetin could represent a new therapeutic strategy to control endometriosis perhaps by targeting the MC-derived NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome pathway and oxidative stress.
Effects of l-Arginine Plus Vitamin C Supplementation on l-Arginine Metabolism in Adults with Long COVID: Secondary Analysis of a Randomized Clinical Trial
Authors: Calvani Riccardo , Gervasoni Jacopo , Picca Anna , Ciciarello Francesca , Galluzzo Vincenzo , Coelho-Júnior Hélio José , Di Mario Clara , Gremese Elisa , Lomuscio Sara , Paglionico Anna Maria , Santucci Lavinia , Tolusso Barbara , Urbani Andrea , Marini Federico , Marzetti Emanuele , Landi Francesco , Tosato Matteo
Published: March 2023

Altered l-arginine metabolism has been described in patients with COVID-19 and has been associated with immune and vascular dysfunction. In the present investigation, we determined the serum concentrations of l-arginine, citrulline, ornithine, monomethyl-l-arginine (MMA), and symmetric and asymmetric dimethylarginine (SDMA, ADMA) in adults with long COVID at baseline and after 28-days of l-arginine plus vitamin C or placebo supplementation enrolled in a randomized clinical trial, compared with a group of adults without previous history of SARS-CoV-2-infection. l-arginine-derived markers of nitric oxide (NO) bioavailability (i.e., l-arginine/ADMA, l-arginine/citrulline+ornithine, and l-arginine/ornithine) were also assayed. Partial least squares discriminant analysis (PLS–DA) models were built to characterize systemic l-arginine metabolism and assess the effects of the supplementation. PLS–DA allowed discrimination of participants with long COVID from healthy controls with 80.2 ± 3.0% accuracy. Lower markers of NO bioavailability were found in participants with long COVID. After 28 days of l-arginine plus vitamin C supplementation, serum l-arginine concentrations and l-arginine/ADMA increased significantly compared with placebo. This supplement may therefore be proposed as a remedy to increase NO bioavailability in people with long COVID.
Ovariectomy Exacerbates Acute Ethanol-Induced Tachycardia: Role of Nitric Oxide and NMDA Receptors in the Rostral Ventrolateral Medulla
Authors: Situmorang Jiro Hasegawa , Lin Hsun-Hsun , Islam Md Sharyful , Lai Chih-Chia
Published: March 2023

Ethanol consumption influences cardiovascular functions. In humans, acute consumption of ethanol causes dose-dependent tachycardia. Our previous study showed that ethanol-induced tachycardia might involve decreased nitric oxide (NO) signaling in the brain’s medulla. NMDA receptors, another important target of ethanol, are one of the upstream signals of nitric oxide. Reports showed the modulation of NMDA receptor function by estrogen or estrogen receptors. The present study aims to examine the hypothesis that depletion of estrogen by ovariectomy (OVX) might modulate ethanol-induced tachycardia by regulating NMDA receptor function and NO signaling in the cardiovascular regulatory nucleus of the brain. Ethanol (3.2 g/kg, 40% v/v, 10 mL/kg) or saline (10 mL/kg) was administered by oral gavage in sham or OVX female Sprague-Dawley (SD) rats. The blood pressure (BP) and heart rate (HR) were measured using the tail-cuff method. The levels of phosphoserine 896 of the GluN1 subunit (pGluN1-serine 896) and NMDA GluN1 subunits (GluN1) were determined by immunohistochemistry. The expressions of nitric oxide synthase (NOS) and estrogen receptors in the tissue were measured by Western blotting. Nitric oxide contents were measured as total nitrate-nitrite by colorimetric assay kit. In a 2-h observation, there was no significant change in BP between the saline and ethanol groups. However, compared with saline, ethanol caused an increase in HR (tachycardia) in sham control or OVX rats. Interestingly, ethanol produced more significant tachycardia in the OVX group than in the sham control group. Nitric oxide levels were lower in the area of the rostral ventrolateral medulla (RVLM) 60 min following ethanol administration in OVX compared with sham control, without significant changes in the expression of NOS and estrogen receptors (ERα and ERβ). In addition, a decrease in the immunoreactivity of pGluN1-serine 896, without significant changes in GluN1, was found in neurons of RVLM 40 min following ethanol administration in OVX compared with sham control. Our results suggest that depletion of estradiol (E2) by OVX might exacerbate the tachycardia following ethanol administration, the underlying mechanism of which might be associated with decreased NMDA receptor function and NO level in the RVLM.
Utilization of Supervised Machine Learning to Understand Kinase Inhibitor Toxophore Profiles
Authors: Bieberich Andrew A. , Asquith Christopher R. M.
Published: March 2023

There have been more than 70 FDA-approved drugs to target the ATP binding site of kinases, mainly in the field of oncology. These compounds are usually developed to target specific kinases, but in practice, most of these drugs are multi-kinase inhibitors that leverage the conserved nature of the ATP pocket across multiple kinases to increase their clinical efficacy. To utilize kinase inhibitors in targeted therapy and outside of oncology, a narrower kinome profile and an understanding of the toxicity profile is imperative. This is essential when considering treating chronic diseases with kinase targets, including neurodegeneration and inflammation. This will require the exploration of inhibitor chemical space and an in-depth understanding of off-target interactions. We have developed an early pipeline toxicity screening platform that uses supervised machine learning (ML) to classify test compounds’ cell stress phenotypes relative to a training set of on-market and withdrawn drugs. Here, we apply it to better understand the toxophores of some literature kinase inhibitor scaffolds, looking specifically at a series of 4-anilinoquinoline and 4-anilinoquinazoline model libraries.
Extracting Self-Reported COVID-19 Symptom Tweets and Twitter Movement Mobility Origin/Destination Matrices to Inform Disease Models
Authors: Rosato Conor , Moore Robert E. , Carter Matthew , Heap John , Harris John , Storopoli Jose , Maskell Simon
Published: March 2023

The emergence of the novel coronavirus (COVID-19) generated a need to quickly and accurately assemble up-to-date information related to its spread. In this research article, we propose two methods in which Twitter is useful when modelling the spread of COVID-19: (1) machine learning algorithms trained in English, Spanish, German, Portuguese and Italian are used to identify symptomatic individuals derived from Twitter. Using the geo-location attached to each tweet, we map users to a geographic location to produce a time-series of potential symptomatic individuals. We calibrate an extended SEIRD epidemiological model with combinations of low-latency data feeds, including the symptomatic tweets, with death data and infer the parameters of the model. We then evaluate the usefulness of the data feeds when making predictions of daily deaths in 50 US States, 16 Latin American countries, 2 European countries and 7 NHS (National Health Service) regions in the UK. We show that using symptomatic tweets can result in a 6% and 17% increase in mean squared error accuracy, on average, when predicting COVID-19 deaths in US States and the rest of the world, respectively, compared to using solely death data. (2) Origin/destination (O/D) matrices, for movements between seven NHS regions, are constructed by determining when a user has tweeted twice in a 24 h period in two different locations. We show that increasing and decreasing a social connectivity parameter within an SIR model affects the rate of spread of a disease.
Magnitude and Extent of Helicoverpa zea Resistance Levels to Cry1Ac and Cry2Ab2 across the Southeastern USA
Authors: Reisig Dominic , Buntin G. David , Greene Jeremy K. , Paula-Moraes Silvana V. , Reay-Jones Francis , Roberts Phillip , Smith Ron , Taylor Sally V.
Published: March 2023

After resistance is first detected, continued resistance monitoring can inform decisions on how to effectively manage resistant populations. We monitored for resistance to Cry1Ac (2018 and 2019) and Cry2Ab2 (2019) from southeastern USA populations of Helicoverpa zea. We collected larvae from various plant hosts, sib-mated the adults, and tested neonates using diet-overlay bioassays and compared them to susceptible populations for resistance estimates. We also compared LC50 values with larval survival, weight and larval inhibition at the highest dose tested using regression, and found that LC50 values were negatively correlated with survival for both proteins. Finally, we compared resistance rations between Cry1Ac and Cry2Ab2 during 2019. Some populations were resistant to Cry1Ac, and most were resistant to CryAb2; Cry1Ac resistance ratios were lower than Cry2Ab2 during 2019. Survival was positively correlated with larval weight inhibition for Cry2Ab. This contrasts with other studies in both the mid-southern and southeastern USA, where resistance to Cry1Ac, Cry1A.105, and Cry2Ab2 increased over time and was found in a majority of populations. This indicates that cotton expressing Cry proteins in the southeastern USA was at variable risk for damage in this region.
Antiplatelet Strategies for Older Patients with Acute Coronary Syndromes: Finding Directions in a Low-Evidence Field
Authors: De Servi Stefano , Landi Antonio , Savonitto Stefano , Morici Nuccia , De Luca Leonardo , Montalto Claudio , Crimi Gabriele , De Rosa Roberta , De Luca Giuseppe
Published: March 2023

Patients ≥ 75 years of age account for about one third of hospitalizations for acute coronary syndromes (ACS). Since the latest European Society of Cardiology guidelines recommend that older ACS patients use the same diagnostic and interventional strategies used by the younger ones, most elderly patients are currently treated invasively. Therefore, an appropriate dual antiplatelet therapy (DAPT) is indicated as part of the secondary prevention strategy to be implemented in such patients. The choice of the composition and duration of DAPT should be tailored on an individual basis, after careful assessment of the thrombotic and bleeding risk of each patient. Advanced age is a main risk factor for bleeding. Recent data show that in patients of high bleeding risk short DAPT (1 to 3 months) is associated with decreased bleeding complications and similar thrombotic events, as compared to standard 12-month DAPT. Clopidogrel seems the preferable P2Y12 inhibitor, due to a better safety profile than ticagrelor. When the bleeding risk is associated with a high thrombotic risk (a circumstance present in about two thirds of older ACS patients) it is important to tailor the treatment by taking into account the fact that the thrombotic risk is high during the first months after the index event and then wanes gradually over time, whereas the bleeding risk remains constant. Under these circumstances, a de-escalation strategy seems reasonable, starting with DAPT that includes aspirin and low-dose prasugrel (a more potent and reliable P2Y12 inhibitor than clopidogrel) then switching after 2–3 months to DAPT with aspirin and clopidogrel for up to 12 months.
Celiac Disease and Cardiovascular Risk: A Retrospective Case-Control Study
Authors: Dore Maria Pina , Mereu Sandro , Saba Pier Sergio , Portoghese Michele , Pes Giovanni Mario
Published: March 2023

Background: The association of celiac disease (CD) with premature atherosclerosis, including increased carotid artery intima-media thickness and cardiovascular disease (CVD), is controversial. The aim of this study was to investigate this relationship. Methods: Clinical records of patients from Northern Sardinia referred to the Gastroenterology section of the Department of Medicine, University of Sassari, Italy, were analyzed. Unadjusted and adjusted odds ratios (ORs) for CVD with their 95% confidence intervals (CIs) were calculated according to established risk factors, including age, sex, diabetes, dyslipidemia, overweight/obesity, blood hypertension, and cigarette smoking, as well as a possible risk factor such as H. pylori infection. Results: In a total of 8495 patients (mean age 52.1 ± 17.3 years; 64.7% females), 2504 reported a diagnosis of CVD and 632 of CD. Logistic regression analysis showed a significantly reduced risk of CVD among patients with CD (OR 0.30, 95% CI 0.22–0.41). Moreover, the long duration of the gluten-free diet (GFD) was able to lower the risk of CVD in celiac patients. Finally, CD significantly decreased the frequency of carotid plaques (11.8% vs. 40.1%, p < 0.001). Conclusions. Our retrospective study demonstrated that CD reduces the risk of CVD in general and more specifically of carotid lesions after adjusting for potential confounders, especially in those on GFD for a long time.
Evaluation and Optimization of the Layout of Community Public Service Facilities for the Elderly: A Case Study of Hangzhou
Authors: Li Yonghua , Ran Qinchuan , Yao Song , Ding Likun
Published: March 2023

Community public service facilities have a primary supportive role in the health of the elderly. Under the background of global aging, it has become vital to evaluate the elderly-adaptability of their layouts. Based on the supply and demand interaction perspective, this study used the questionnaire-AHP-2SFCA method for this purpose. Firstly, taking the six main districts of Hangzhou as an example, we analyzed the spatial distribution characteristics of the elderly population, and a weight index table of the health importance of public service facilities was constructed using a questionnaire survey and the AHP method. Secondly, the improved 2SFCA was used to analyze the accessibility of public service facilities in Hangzhou, and combined with the weight index table, the elderly-adaptability of public service facilities in the community life circle was comprehensively evaluated. Finally, the demands of the elderly and the supply of public service facilities in the same region were superimposed to study the differential pattern of supply and demand. The results showed the following: (1) The communities with the largest elderly population are mainly concentrated in Shangcheng District, Xiacheng District, the north of Gongshu District, the west of Jianggan District, and the north of Binjiang District. (2) Green space facilities in parks are most important to the health of the elderly, with a weight of 0.46. (3) The overall evaluation results of the community life circle in the study area were good, and the proportion of areas above the medium level was more than 50%. This showed that the concepts of “neighborhood center” and “big community elderly care” in Hangzhou have achieved initial positive results. (4) Based on the interaction between supply and demand, the research area can be divided into four patterns: supply and demand balance, supply shortage, demand gap, and low supply and demand. The results of this study will help to improve the layout and aging-friendly status of community life circle facilities in Hangzhou, and provide information for other aging cities.
Effects of N-Alkanol Adsorption on Bubble Acceleration and Local Velocities in Solutions of the Homologous Series from Ethanol to N-Decanol
Authors: Krzan Marcel , Chattopadhyay Pradipta , Orvalho Sandra , Zednikova Maria
Published: March 2023

The influence of n-alkanol (C2–C10) water solutions on bubble motion was studied in a wide range of concentrations. Initial bubble acceleration, as well as local, maximal and terminal velocities during motion were studied as a function of motion time. Generally, two types of velocity profiles were observed. For low surface-active alkanols (C2–C4), bubble acceleration and terminal velocities diminished with the increase in solution concentration and adsorption coverage. No maximum velocities were distinguished. The situation is much more complicated for higher surface-active alkanols (C5–C10). In low and medium solution concentrations, bubbles detached from the capillary with acceleration comparable to gravitational acceleration, and profiles of the local velocities showed maxima. The terminal velocity of bubbles decreased with increasing adsorption coverage. The heights and widths of the maximum diminished with increasing solution concentration. Much lower initial acceleration values and no maxima presence were observed in the case of the highest n-alkanol concentrations (C5–C10). Nevertheless, in these solutions, the observed terminal velocities were significantly higher than in the case of bubbles moving in solutions of lower concentration (C2–C4). The observed differences were explained by different states of the adsorption layer in the studied solutions, leading to varying degrees of immobilization of the bubble interface, which generates other hydrodynamic conditions of bubble motion.
Grinding Burn Detection via Magnetic Barkhausen Noise Analysis Independently of Induction Hardened Depth
Authors: Gurruchaga Kizkitza , Lasaosa Aitor , Artetxe Itsaso , Martínez-de-Guerenu Ane
Published: March 2023

The electromagnetic technique based on magnetic Barkhausen noise (MBN) can be used to control the quality of ball screw shafts non-destructively, although identifying any slight grinding burns independently of induction-hardened depth remains a challenge. The capacity to detect slight grinding burns was studied using a set of ball screw shafts manufactured by means of different induction hardening treatments and different grinding conditions (some of them under abnormal conditions for the purpose of generating grinding burns), and MBN measurements were taken in the whole group of ball screw shafts. Additionally, some of them were tested using two different MBN systems in order to better understand the effect of the slight grinding burns, while Vickers microhardness and nanohardness measurements were taken in selected samples. To detect the grinding burns (both slight anddata intense) with varying depths of the hardened layer, a multiparametric analysis of the MBN signal is proposed using the main parameters of the MBN two-peak envelope. At first, the samples are classified into groups depending on their hardened layer depth, estimated using the intensity of the magnetic field measured on the first peak (H1) parameter, and the threshold functions of two parameters (the minimum amplitude between the peaks of the MBN envelope (MIN) and the amplitude of the second peak (P2)) are then determined to detect the slight grinding burns for the different groups.
Zeolite Composite Materials from Fly Ash: An Assessment of Physicochemical and Adsorption Properties
Authors: Mokrzycki Jakub , Franus Wojciech , Panek Rafał , Sobczyk Maciej , Rusiniak Piotr , Szerement Justyna , Jarosz Renata , Marcińska-Mazur Lidia , Bajda Tomasz , Mierzwa-Hersztek Monika
Published: March 2023

Waste fly ash, with both low (with the addition of vermiculite) and high contents of unburned coal, were subjected to hydrothermal syntheses aiming to obtain zeolite composite materials—zeolite + vermiculite (NaX–Ver) and zeolite + unburned carbon (NaX–C). The composites were compared with parent zeolite obtained from waste fly ash with a low content of unburned carbon (NaX–FA). In this study, the physicochemical characteristics of the obtained materials were evaluated. The potential application of the investigated zeolites for the adsorption of ammonium ions from aqueous solutions was determined. Composite NaX–Ver and parent zeolite NaX–FA were characterized by comparable adsorption capacities toward ammonium ions of 38.46 and 40.00 mg (NH4+) g−1, respectively. The nearly 2-fold lower adsorption capacity of composite NaX–C (21.05 mg (NH4+) g−1) was probably a result of the lower availability of ion exchange sites within the material. Adsorbents were also regenerated using 1 M NaCl solution at a pH of 10 and subjected to 3 cycles of adsorption–desorption experiments, which proved only a small reduction in adsorption properties. This study follows the current trend of waste utilization (fly ash) and the removal of pollutants from aqueous solutions with respect to their reuse, which remains in line with the goals of the circular economy.
What If Moms Quiet Quit? The Role of Maternity Leave Policy in Working Mothers’ Quiet Quitting Behaviors
Authors: Zhang Tingting , Rodrigue Chloe
Published: March 2023

This study aims to examine the effects of various maternity leave support on the quiet quitting behaviors and mental health conditions of working mothers across industries during the post-pandemic period. Through an empirical survey method of 310 valid responses from a panel data, the study results indicated that working mothers who took maternity leave were less likely to adopt quiet quitting behaviors when they returned to work after childbirth and showed better mental health at work compared to their peers who did not take maternity leave because of childbirth and/or childcare. Additionally, paid maternity leave was not found to have a significant effect on quiet quitting behaviors and mental health of working mothers across industries, but the duration of maternity leave was found as a significant factor in impacting working mothers’ quiet quitting behaviors and their mental health conditions. Moreover, peer workers’ quiet quitting behaviors and supervisors’ support for childcare (e.g., flexible work schedule) were found significantly to improve working mothers’ quiet quitting tendencies at work. Lastly, there exist significant differences in age and race in the working mothers’ quiet quitting behaviors at work.
Innovative Tungsten Coatings for an Application in Modern and Future Fusion Devices
Authors: Keller Tom , Litnovsky Andrey , Mauer Georg , Linsmeier Christian , Guillon Olivier
Published: March 2023

Tungsten is foreseen presently as the plasma-facing material for divertors in fusion power plants. In order to achieve durable operation of divertors of current fusion reactors, an efficient way of maintaining the divertor functionality is needed. A system capable of in situ tungsten coating of the divertor via low-pressure plasma spraying was proposed to maintain the divertor integrity. In this work, tungsten was deposited on NB31 carbon fibre composite substrates using the low-pressure plasma spraying technology to evaluate the feasibility of this technique. The thickness, porosity, composition, adhesion, and microstructure of the coatings were investigated by scanning electron microscopy image analysis and energy dispersive spectroscopy. Based on the initial results, the spray parameters were iteratively improved in a campaign-based study. The coatings exhibited improving properties through an adjusting of the carrier gas flow, the scanning speed, and the spray distance. By lowering the carrier gas flow, the porosity of the coatings was reduced, resulting in coatings of 98% bulk density. Adjusting the carrier gas flow reduced the amount of semi-molten particles in the coatings significantly. A decrease in both scanning speed and spray distance increased the substrate’s temperature, which led to better adhesion and porosity.
Multi-Channel MEMS-FAIMS Gas Sensor for VOCs Detection
Authors: Zhao Zhujie , Lei Cheng , Liang Ting , Zhang Junna , Liu Yuqiao , Ghaffar Abdul , Xiong Jijun
Published: March 2023

Aimed at the problems of a large equipment size, long time and high price of environmental VOC gas detection, the FAIMS-VOC gas sensor was designed and prepared according to the principle that the ionization energy of the common VOC gas is less than 10.6 eV. The sensor is small in size, fast in detection, low in power consumption, and can work continuously. The sensor was fabricated through the MEMS process, a specific process which included photolithography, etching, anodic bonding, etc. The sensor is 5160 μm long, 5300 μm wide and 800 μm high. We built a test system to detect two typical VOC gases: isobutylene and acetone. The results show that in the detection of isobutylene gas and acetone gas, the sensor voltage value changes with the change of gas concentration. The linearity of testing isobutylene is 0.961, and the linearity of testing acetone is 0.987. When the isobutylene gas concentration is 50 ppm, the response time is 8 s and the recovery time is 6 s; when the acetone gas concentration is 50 ppm, the response time is 9 s and the recovery time is 10 s. In addition, the sensor demonstrates good repeatability and stability, which are conducive to the detection of VOCs in the environment.
Conceptual Navigation and Positioning Solution for the Upcoming Lunar Mining and Settlement Missions Based on the Earth’s Mining Experiences: Lunar Regional Navigation Transceiver System
Authors: Ignjatović Stupar Danijela , Ogrizović Vukan , Rošer Janez , Poslončec-Petrić Vesna , Vižintin Goran
Published: March 2023

Precise drilling and excavation in future Lunar mining sites as well as in building habitats areas will be supported by robotized instrumentation. To ensure accurate positioning of facilities or structures, customized surveying instruments will be used to perform measurements needed for calculating locations of surveyed objects. Precise positioning in unexplored areas is difficult, even on the Earth, with all available support. This issue becomes even more complex on the Moon’s surface, considering environmental conditions and the absence of Earth logistics. This paper solves a problem of centimeter-precision positioning on the Moon’s surface. The solution is called Lunar Regional Navigation Transceiver System (LRNTS). It is based on a network of transceiver facilities, holding onboard both navigation transmitters and receivers. Transmitting modules of LRNTS act in the same way as the Global Navigation Satellite Systems (GNSS) space segment, sending navigation messages to the receivers. Receiving modules are needed for self-calibration of LRNTS to calculate their coordinates. In this paper, 12 different LRNTS-simulated configuration setups within Shackleton Crater are tested against positioning accuracy and visibility along the crater. The results show that LRNTS of nine transceivers can achieve sub-centimeter horizontal and better than 2 cm vertical accuracy, with consistent visibility of six and more transceivers throughout the Shackleton Crater.
Anti-Inflammatory Activity of Panax notoginseng Flower Saponins Quantified Using LC/MS/MS
Authors: Liu Junchen , Wu Yuehang , Ma Wenrui , Zhang Hongyan , Meng Xianyao , Zhang Huirong , Guo Miaomiao , Ling Xiao , Li Li
Published: March 2023

Panax notoginseng (Burk) F. H. Chen is a traditional Chinese medicinal and edible plant. However, Panax notoginseng flower (PNF) is rarely used. Therefore, the purpose of this study was to explore the main saponins and the anti-inflammatory bioactivity of PNF saponins (PNFS). We explored the regulation of cyclooxygenase 2 (COX–2), a key mediator of inflammatory pathways, in human keratinocyte cells treated with PNFS. A cell model of UVB-irradiation-induced inflammation was established to determine the influence of PNFS on inflammatory factors and their relationship with LL–37 expression. An enzyme-linked immunosorbent assay and Western blotting analysis were used to detect the production of inflammatory factors and LL37. Finally, liquid chromatography–tandem mass spectrometry was employed to quantify the main active components (ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rg1, and notoginsenoside R1) in PNF. The results show that PNFS substantially inhibited COX–2 activity and downregulated the production of inflammatory factors, indicating that they can be used to reduce skin inflammation. PNFS also increased the expression of LL-37. The contents of ginsenosides Rb1, Rb2, Rb3, Rc, and Rd in PNF were much higher than those of Rg1, and notoginsenoside R1. This paper provides data in support of the application of PNF in cosmetics.
A Self-Powered High-Responsivity, Fast-Response-Speed Solar-Blind Ultraviolet Photodetector Based on CuO/β-Ga2O3 Heterojunction with Built-In Potential Control
Authors: Park Sangbin , Yoon Younghwa , Kim Hyungmin , Park Taejun , Kim Kyunghwan , Hong Jeongsoo
Published: March 2023

Controlling built-in potential can enhance the photoresponse performance of self-powered photodetectors. Among the methods for controlling the built-in potential of self-powered devices, postannealing is simpler, more efficient, and less expensive than ion doping and alternative material research. In this study, a CuO film was deposited on a β-Ga2O3 epitaxial layer via reactive sputtering with an FTS system, and a self-powered solar-blind photodetector was fabricated through a CuO/β-Ga2O3 heterojunction and postannealed at different temperatures. The postannealing process reduced the defects and dislocations at the interface between each layer and affected the electrical and structural properties of the CuO film. After postannealing at 300 °C, the carrier concentration of the CuO film increased from 4.24 × 1018 to 1.36 × 1020 cm−3, bringing the Fermi level toward the valence band of the CuO film and increasing the built-in potential of the CuO/β-Ga2O3 heterojunction. Thus, the photogenerated carriers were rapidly separated, increasing the sensitivity and response speed of the photodetector. The as-fabricated photodetector with 300 °C postannealing exhibited a photo-to-dark current ratio of 1.07 × 103; responsivity and detectivity of 30.3 mA/W and 1.10 × 1012 Jones, respectively; and fast rise and decay times of 12 ms and 14 ms, respectively. After three months of storage in an open-air space, the photocurrent density of the photodetector was maintained, indicating good stability with aging. These results suggest that the photocharacteristics of CuO/β-Ga2O3 heterojunction self-powered solar-blind photodetectors can be improved through built-in potential control using a postannealing process.
Antioxidant and Anti-Inflammatory Effects of Oral Supplementation with a Highly-Concentrated Docosahexaenoic Acid (DHA) Triglyceride in Patients with Keratoconus: A Randomized Controlled Preliminary Study
Authors: Peris-Martínez Cristina , Piá-Ludeña José Vicente , Rog-Revert María José , Fernández-López Ester , Domingo Joan Carles
Published: March 2023

A prospective, randomized, single-center preliminary study was performed in patients with keratoconus stages I–III (Amsler–Krumeich), who received a high rich docosahexaenoic acid (DHA) (1000 mg/day) supplement for 3 months versus untreated patients. One eye per patient was evaluated. Thirty-four patients were recruited (75% men, mean age 31 years), with 15 randomized to the control group and 19 to the DHA-treated group. Corneal topography variables and plasma biomarkers of oxidative stress and inflammatory status were evaluated. A panel of fatty acids in blood samples was also assessed. There were significant between-group differences in the astigmatism axis, asphericity coefficient, and intraocular pressure in favor of the DHA group. Additionally, between-group significant differences in total antioxidant capacity (TAC), malondialdehyde (MDA), free glutathione (GSH) and GSH/GSSG ratio, as well as reduced values of inflammatory markers, including interleukin (IL)-4, IL-6, and vascular endothelial growth factor (VEGF-A) were found. These preliminary findings support the usefulness of the antioxidant and anti-inflammatory effects of DHA supplementation for targeting underlying pathophysiological mechanisms of keratoconus. Prolonged duration of DHA supplementation may be needed to detect more noticeable clinical changes in corneal topography.
Compaction Behavior of Co-Amorphous Systems
Authors: Sørensen Cecilie-Mathilde , Rantanen Jukka , Grohganz Holger
Published: March 2023

Co-amorphous systems have been shown to be a promising strategy to address the poor water solubility of many drug candidates. However, little is known about the effect of downstream processing-induced stress on these systems. The aim of this study is to investigate the compaction properties of co-amorphous materials and their solid-state stability upon compaction. Model systems of co-amorphous materials consisting of carvedilol and the two co-formers aspartic acid and tryptophan were produced via spray drying. The solid state of matter was characterized using XRPD, DSC, and SEM. Co-amorphous tablets were produced with a compaction simulator, using varying amounts of MCC in the range of 24 to 95.5% (w/w) as a filler, and showed high compressibility. Higher contents of co-amorphous material led to an increase in the disintegration time; however, the tensile strength remained rather constant at around 3.8 MPa. No indication of recrystallization of the co-amorphous systems was observed. This study found that co-amorphous systems are able to deform plastically under pressure and form mechanically stable tablets.
Prediction of Process Parameters for Ultra-Precision Optical Processing Based on Dual-Stacked LSTM
Authors: Ang Pengzhi , Yang Minghong , Chen Jun , Cao Jun , Zhu Qiuyu
Published: March 2023

High-precision and large-aperture optical components have important applications in modern optics and optoelectronics. However, the traditional continuous polishing technology of optical components relies heavily on the processing experience of the processing personnel. The surface shape of the pitch lap is judged by frequent offline measurement of the surface shape of the processing workpiece, and then the processing personnel judges how to adjust the next process parameters through their own experience, which leads to uncertainty of processing time and low processing efficiency. In this paper, based on the historical processing data, including the surface parameters of workpieces and process parameters before and after each processing, a machine learning-based prediction method of process parameters is proposed. At first, taking the surface shape of the pitch lap as the hidden parameter of the model, a time-series mathematical model of the forward and reverse processing processes is constructed. Theoretical and experimental results show that the prediction method in this paper can effectively reduce the processing time and improve the stability of the workpiece quality.