bluuebunny/crossref_metadata_2025 huggingface dataset:
Exogenous Fecal Microbial Transplantation Alters Fearfulness, Intestinal Morphology, and Gut Microbiota in Broilers
Authors: Yan Chao , Xiao Jinlong , Li Zhiwei , Liu Hao , Zhao Xinjie , Liu Jian , Chen Siyu , Zhao Xingbo
Published: October 2021

Fecal microbiota transplantation (FMT) documented transplanting a donor fecal sample to a receipt individual for a desired physiologic effect. However, whether the gut microbiota construction, intestinal maturation, and behavioral plasticity are modulated by FMT during the early life of broilers is waiting for verification. To evaluate the role of transfer of fecal microbiota from aged broilers donor (BD) to another individual, 96 birds were equally divided into a check (CK, control) group and a broiler recipient (BR) group. FMT was conducted daily from 5 to 12 days of age to determine the future impact on body weight, behavior, intestinal development, and gut microbiota. Results indicated that fearfulness in the CK group was higher than the BR group in both the behavioral tests (p < 0.05). The muscularis mucosa, thickness of muscle layer, and thickness of serous membrane layer in the BR group were higher compared with those of the CK group in the jejunum (p < 0.05). In the gut microbiota, Shannon diversity showed no difference, while beta diversity presented a difference in principal coordination analysis (PCoA) between the CK and BR groups. At the phylum level, the relative abundance of Lentisphaerae in the CK group was lower than the BR (p = 0.052) and BD (p = 0.054) groups. The relative abundance of Tenericutes in the BD group was higher than that in the CK and BR groups (p < 0.05). At the genus level, Megamonas in the CK group was higher than the BR (p = 0.06) and BD (p < 0.05) groups. In the BR group, the functional capabilities of microbial communities analyzed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were increased in the glutamatergic synapse and N-glycan biosynthesis pathways in comparison with the CK and BD groups (p < 0.05). Some characteristics of gut microbiota in the donor chickens could be transferred to recipient chickens by FMT. In conclusion, exogenous FMT as a probiotic-like administration might be an efficient way to improve the physiology and behavior of chickens. Notably, the role of microbiota for various individuals and periods remains undefined, and the mechanism of microbiota on behaviors still needs further investigation.
The Short Form of the Glasgow Composite Measure Pain Scale in Post-operative Analgesia Studies in Dogs: A Scoping Review
Authors: Testa Barbara , Reid Jacqueline , Scott Marian E. , Murison Pamela J. , Bell Andrew M.
Published: October 2021

The measurement and treatment of acute pain in animals is essential from a welfare perspective. Valid pain-related outcome measures are also crucial for ensuring reliable and translatable findings in veterinary clinical trials. The short form of the Glasgow Composite Measure Pain Scale (CMPS-SF) is a multi-item behavioral pain assessment tool, developed and validated using a psychometric approach, to measure acute pain in the dog. Here we conduct a scoping review to identify prospective research studies that have used the CMPS-SF. We aim to describe the contexts in which it has been used, verify the correct use of the scale, and examine whether these studies are well-designed and adequately powered. We identify 114 eligible studies, indicating widespread use of the scale. We also document a limited number of modifications to the scale and intervention level, which would alter its validity. A variety of methods, with no consensus, were used to analyse data derived from the scale. However, we also find many deficiencies in reporting of experimental design in terms of the observers used, the underlying hypothesis of the research, the statement of primary outcome, and the use ofa priorisample size calculations. These deficiencies may predispose to both type I and type II statistical errors in the small animal pain literature. We recommend more robust use of the scale and derived data to ensure success of future studies using the tool ensuring reliable and translatable outcomes.
Thoughts on the Dilemma of When to Introduce the Euro in Hungary
Authors: Gottfried Péter
Published: October 2021

In its EU accession treaty, Hungary committed to introducing the euro without a legally binding deadline. The question is therefore not whether the country will introduce the common currency, but when it will do so, and what factors play a role in the decision. Developments in recent years have confirmed that the euro system is able to weather a crisis, but also highlight that the euro does not in itself guarantee sustainable convergence. In addition to accession, members’ rights and obligations have also changed considerably, and these changes have not been completed. There are examples for successful economic policies without the euro and also for frustrated growth with the euro. Only one area has been identified where the advantages of membership are indisputable: yield spreads. In today’s international environment, this is much less important than in the past, but it is impossible to know how long this situation will last. Accordingly, the Hungarian strategy should target sustainable convergence, rather than the introduction of the euro. If the country can substantially reverse the increase in the government deficit and debt and keep them low, it would be worth waiting until the development paths related to the euro are outlined more clearly, while continuing with convergence. If this is not possible, the option to join the euro area as soon as possible should be preferred, which offers greater security but less room for autonomous manoeuvre.
A Real-Time Realization Method for the Pneumatic Positioning System of the Industrial Automated Production Line Using Low-Cost On–Off Valves
Authors: Lin Zhonglin , Xie Qi , Qian Qiang , Zhang Tianhong , Zhang Jiaming , Zhuang Jiaquan , Wang Weixiong
Published: October 2021

In the industrial automated production line, how to use the existing low-cost pneumatic equipment to obtain the best positioning effect has become a significant engineering problem. In this paper, a differential switching method is proposed in a pneumatic servo system consisting of four low-cost on–off valves for more precise control and lower prices. All valves are simultaneously open at the initial stage of each control period, and the differential closing time of the desired valves is calculated through the theoretical models. A sliding mode controller is applied with the proposed method, and the system stability is proven. The real-time control setup including three software layers is proposed to implement the algorithm. Several experiments are performed on a real-time embedded controller. Average 0.83% overshoot and 0.18 mm steady-state error are observed in the step response experiment. The highest frequency of sine wave that can be tracked is 1 Hz, and the average error is 1.68 mm. The maximum steady-state error is about 0.5 mm in the step response under payloads of 5.25 kg. All the simulation and experimental results prove the effectiveness of the control method.
Orbital Design and Control for Jupiter-Observation Spacecraft
Authors: Jiang Chunsheng , Liu Yongjie , Jiang Yu , Li Hengnian
Published: October 2021

This paper investigates the evolution of orbits around Jupiter and designs a sun-synchronous repeating ground track orbit. In the dynamical models, the leading terms of the Jupiter’s oblateness are J2 and J4 terms. A reasonable range of ground track repetition parameter Q is given and the best observation orbit elements are selected. Meanwhile, the disturbing function acting on the navigation spacecraft is the atmospheric drag and the third body. The law of altitude decay of the spacecraft’s semimajor orbit axis caused by the atmospheric drag is studied, and the inclination perturbation caused by the sun’s gravity is analyzed. This paper designs a semimajor axis compensation strategy to maintain the orbit’s repeatability and proposes an initial inclination prebiased strategy to limit the local time at the descending node in a permitted range. In particular, these two methods are combined in the context of sun-synchronous repeating ground track orbit for better observation of the surface of Jupiter.
Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
Authors: Vannucchi Francesca , Imperato Valeria , Saran Anabel , Staykov Svetoslav , D’Haen Jan , Sebastiani Luca , Vangronsveld Jaco , Thijs Sofie
Published: October 2021

Seed endophyte inoculation can enhance the plant tolerance to pollutants, which allows plant cultivation on trace element (TE) polluted soils. Methylobacterium sp. CP3 and Kineococcus endophyticus CP19 were tested in vitro for their tolerance to Zn and Cd and their plant growth promotion traits. The in vivo effects of bioaugmentation with individual strains or both strains were tested using two poplar cultivars, Populus deltoides x (P. trichocarpa x P. maximowiczii) ‘Dender’ and ‘Marke’, grown in TE polluted soil for six weeks. Methylobacterium sp. was found to grow on media enriched with 0.4 and 0.8 mM Cd, and both endophytes tolerated 0.6 and 1 mM Zn, due to the presence of genes involved in Zn and Cd tolerance and transport. Methylobacterium sp. showed an extracellular ion sequestration mechanism. Production of indole-3-acetic acid by Methylobacterium sp. and K. endophyticus, as well as phosphorus solubilization by Methylobacterium sp. were observed. Bioaugmentation with both endophytes increased the shoot length of Populus ‘Marke’ and enhanced the Mg uptake in both cultivars. Inoculation with Methylobacterium sp. reduced the bioaccumulation of Zn in ‘Marke’, conferring it an excluder strategy. Methylobacterium sp. and K. endophyticus seemed to improve the plant nutritional status, which can alleviate abiotic stress.
Soilless System with Supplementary LED Light to Obtain a High-Quality Out-of-Season Production of Green Beans
Authors: Signore Angelo , Leoni Beniamino , Palmitessa Onofrio Davide , Santamaria Pietro
Published: October 2021

Green bean (Phaseolus vulgaris L.) is one of the most important sources of vegetable proteins in the world and it is cultivated all year round, but the light availability, during the dark season, limited its growth. Nevertheless, recent studies conducted on greenhouse horticulture demonstrated that, with the application of light emitting diodes (LEDs) as supplementary light (SL) technology, it is possible to overcome this limitation. Consequently, during the experiment conducted, two cultivars of green bean (‘Saporro’ and ‘Maestrale’) were grown with a soilless system in a cold greenhouse during the fall-winter period. To increase the photoperiod and the daily light integral (DLI), early in the morning, four hours of red (R), blue (B) and red+blue (R + B) supplementary light were supplied by LEDs at 180 μmol·m−2·s−1 (PPFD) at plants level. Plants grown under LEDs improved the yield and the gas exchange system compared with the plants grown under natural light; when B light was supplied as a sole source of SL, it increased the dry matter content and the brightness (L*) of the pods. Between the cultivars, ‘Maestrale’ produced 20 g∙plant−1 of pods more than ‘Saporro’ but the latter’s colour was brighter (L*) and greener (a*), and ‘Saporro’ also showed the highest photosynthetic efficiency (ΦPSII). In conclusion, ‘Maestrale’ and ‘Saporro’ obtained encouraging out-of-season yields under different LED spectra, but among those B light seems to improve overall crop performances and pods quality.
Hydrophilic Interaction Liquid Chromatography Coupled with Fluorescence Detection (HILIC-FL) for the Quantitation of Octreotide in Injection Forms
Authors: Doulou Eleni , Kalomiraki Marina , Parla Anthi , Thermos Kyriaki , Chaniotakis Nikos A. , Panderi Irene
Published: October 2021

Octreotide is a synthetic cyclic octapeptide analogue of somatostatin-14. It is mainly administered for the treatment of acromegaly, severe diarrhea, and neuroendocrine neoplasias. In this work, a hydrophilic interaction liquid chromatography (HILIC) method with fluorescence (FL) detection was developed and validated for the quantitation of octreotide in solutions for injection. Chromatographic separation was performed on an XBridge®-HILIC analytical column under isocratic elution with a short chromatographic run time of less than 10 min. The mobile phase consisted of ammonium bicarbonate 8.6 mM (pH 8.1)/acetonitrile 35/65 (v/v). The high sensitivity and selectivity of the fluorescence detection, with the excitation wavelength (λexcitation) set at 280 nm and the emission wavelength set at (λemission) 330 nm, enabled a simple sample preparation procedure that included only dilution steps. The calibration curve showed good linearity with a correlation coefficient greater than 0.998. The method was successfully applied to the analysis of commercially available octreotide injection forms.
Peroxisome Proliferator-Activated Receptor γ, but Not α or G-Protein Coupled Estrogen Receptor Drives Functioning of Postnatal Boar Testis—Next Generation Sequencing Analysis
Authors: Duliban Michal , Pawlicki Piotr , Gurgul Artur , Tuz Ryszard , Arent Zbigniew , Kotula-Balak Malgorzata , Tarasiuk Kazimierz
Published: October 2021

Porcine tissue gene expression is highly similar to the expression of homologous genes in humans. Based on this fact, the studies on porcine tissues can be employed to understand human physiology and to predict or treat diseases. Our prior studies clearly showed that there was a regulatory partnership of the peroxisome proliferator-activated receptor (PPAR) and the G-protein coupled membrane estrogen receptor (GPER) that relied upon the tumorigenesis of human and mouse testicular interstitial cells, as well as the PPAR-estrogen related receptor and GPER–xenoestrogen relationships which affected the functional status of immature boar testes. The main objective of this study was to identify the biological processes and signaling pathways governed by PPARα, PPARγ and GPER in the immature testes of seven-day-old boars after pharmacological receptor ligand treatment. Boar testicular tissues were cultured in an organotypic system with the respective PPARα, PPARγ or GPER antagonists. To evaluate the effect of the individual receptor deprivation in testicular tissue on global gene expression, Next Generation Sequencing was performed. Bioinformatic analysis revealed 382 transcripts with altered expression. While tissues treated with PPARα or GPER antagonists showed little significance in the enrichment analysis, the antagonists challenged with the PPARγ antagonist displayed significant alterations in biological processes such as: drug metabolism, adhesion and tubule development. Diverse disruption in the Notch signaling pathway was also observed. The findings of our study proposed that neither PPARα nor GPER, but PPARγ alone seemed to be the main player in the regulation of boar testes functioning during early the postnatal developmental window.
Effects of Pantothenic Acid Supplementation on Growth Performance, Carcass Traits, Plasma Parameters of Starter White Pekin Ducks Fed a Corn–Soybean Meal Diet
Authors: Tang Jing , Wu Yongbao , Zhang Bo , Qi Zhiguo , Luo Dawei , Hu Jian , Huang Wei , Zhou Zhengkui , Xie Ming , Hou Shuisheng
Published: October 2021

This study aimed to evaluate the effects of different dietary pantothenic acid levels on growth performance, carcass traits, and plasma biochemical parameters of starter Pekin ducks from 1 to 21 days of age, as well as the pantothenic acid requirement of starter ducks. A total of 384 one-day-old male white Pekin ducklings were assigned randomly into 6 dietary treatments, each with 8 replicate pens of 8 ducks. Ducks were fed conventional basal corn–soybean diets containing 8.5, 10.5, 12.5, 14.5, 16.5, and 18.5 mg/kg pantothenic acid for 21 days. Growth depression, poor pantothenic acid status, fasting hypoglycemia, and elevated plasma uric acid (UA) content were observed in the ducks fed the pantothenic acid-deficient basal diet (p < 0.05), and these adverse effects were ameliorated by pantothenic acid supplementation. Among all ducks, the birds fed the basal diet with no supplementation of pantothenic acid had the lowest body weight, average daily weight gain (ADG), average daily feed intake (ADFI), breast meat yield, and plasma pantothenic acid and glucose contents (p < 0.05), and the greatest plasma UA content (p < 0.05). In addition, all these parameters showed a linear or quadratic response as dietary pantothenic acid levels increased (p < 0.05). According to broken-line regression, the pantothenic acid requirements of starter male white Pekin ducks for body weight, ADG, and plasma pantothenic acid content were 13.36, 13.29, and 15.0 mg/kg, respectively. The data potentially provides theoretical support for the utilization of pantothenic acid in duck production.
Pharmacokinetic Behavior and Pharmacokinetic/Pharmacodynamic Integration of Danofloxacin Following Single or Co-Administration with Meloxicam in Healthy Lambs and Lambs with Respiratory Infections
Authors: Ural Mehmet Nihat , Uney Kamil
Published: October 2021

The aim of this study was to determine the pharmacokinetics and pharmacodynamics of danofloxacin (DAN; 6 mg/kg) following subcutaneous administration alone or co-administration with meloxicam (MLX; 1 mg/kg) in healthy lambs and lambs with respiratory infections. The study was carried out using a total of four groups: HD (healthy; n = 6) and ID (infected; n = 7) groups who were administered DAN only, and HDM (healthy; n = 6) and IDM (infected; n = 7) groups who were administered DAN and MLX simultaneously. The plasma concentrations of DAN were determined using high-performance liquid chromatography–UV and analyzed by the non-compartmental method. DAN exhibited a similar elimination half-life in all groups, including both the healthy and infected lambs. The total clearance in the HDM, ID and IDM groups and volume of distribution in the HDM and IDM groups were significantly reduced. MLX in the IDM group significantly increased the area under the curve (AUC) and peak concentration (Cmax) of DAN compared to the HD group. The Mannheimia haemolytica, Escherichia coli, and Streptococcus spp. strains were isolated from bronchoalveolar lavage fluid samples of the infected lambs. When co-administration with meloxicam, DAN at a 6 mg/kg dose can provide optimum values of ƒAUC0–24/MIC (>56 h) and ƒCmax/MIC (>8) for susceptible M. haemolytica isolates with an MIC90 value of 0.25 µg/mL and susceptible E. coli isolates with an MIC value of ≤0.125 µg/mL.
FtsH Sensitizes Methicillin-Resistant Staphylococcus aureus to β-Lactam Antibiotics by Degrading YpfP, a Lipoteichoic Acid Synthesis Enzyme
Authors: Yeo Won-Sik , Jeong Bohyun , Ullah Nimat , Shah Majid , Ali Amjad , Kim Kyeong , Bae Taeok
Published: October 2021

In the Gram-positive pathogen Staphylococcus aureus, FtsH, a membrane-bound metalloprotease, plays a critical role in bacterial virulence and stress resistance. This protease is also known to sensitize methicillin-resistant Staphylococcus aureus (MRSA) to β-lactam antibiotics; however, the molecular mechanism is not known. Here, by the analysis of FtsH substrate mutants, we found that FtsH sensitizes MRSA specifically to β-lactams by degrading YpfP, the enzyme synthesizing the anchor molecule for lipoteichoic acid (LTA). Both the overexpression of FtsH and the disruption of ypfP-sensitized MRSA to β-lactams were observed. The knockout mutation in ftsH and ypfP increased the thickness of the cell wall. The β-lactam sensitization coincided with the production of aberrantly large LTA molecules. The combination of three mutations in the rpoC, vraB, and SAUSA300_2133 genes blocked the β-lactam-sensitizing effect of FtsH. Murine infection with the ypfP mutant could be treated by oxacillin, a β-lactam antibiotic ineffective against MRSA; however, the effective concentration of oxacillin differed depending on the S. aureus strain. Our study demonstrated that the β-lactam sensitizing effect of FtsH is due to its digestion of YpfP. It also suggests that the larger LTA molecules are responsible for the β-lactam sensitization phenotype, and YpfP is a viable target for developing novel anti-MRSA drugs.
RIP3 Associates with RIP1, TRIF, MAVS, and Also IRF3/7 in Host Innate Immune Signaling in Large Yellow Croaker Larimichthys crocea
Authors: Zou Pengfei , Li Kaiqing , Li Ying , Shen Yingjia , Zhang Ziping , Wang Yilei
Published: October 2021

Receptor-interacting protein 3 (RIP3) has been demonstrated to be a key regulator not only in cell death pathways including apoptosis and necroptosis but also in inflammation and host immune responses. In this study, a RIP3 ortholog named Lc-RIP3 is identified in large yellow croaker (Larimichthys crocea). The open reading frame (ORF) of Lc-RIP3 is 1524 bp long and encodes a protein of 507 amino acids (aa). The deduced Lc-RIP3 protein has an N-terminal kinase domain and a C-terminal RHIM domain, and the genome organization of Lc-RIP3 is conserved in teleosts with 12 exons and 11 introns but is different from that in mammals, which comprises 10 exons and 9 introns. Confocal microscopy revealed that Lc-RIP3 is a cytosolic protein. The expression analysis at the mRNA level indicated that Lc-RIP3 is ubiquitously distributed in various tissues/organs, and could be up-regulated under poly I:C, LPS, PGN, and Pseudomonas plecoglossicida stimulation in vivo. Notably, Lc-RIP3 could induce NF-κB but not IRF3 activation. In addition, Lc-RIP3 co-expression with Lc-TRIF, Lc-MAVS, or Lc-IRF3 significantly abolishes the activation of NF-κB but enhances the induction of IRF3 activity. Moreover, NF-κB activity could be up-regulated when Lc-RIP3 is co-expressed with Lc-RIP1 or Lc-IRF7. These results collectively indicate that Lc-RIP3 acts as an important regulator in host innate immune signaling in teleosts.
Yogurt Fortification by the Addition of Microencapsulated Stripped Weakfish (Cynoscion guatucupa) Protein Hydrolysate
Authors: Lima Karina Oliveira , da Rocha Meritaine , Alemán Ailén , López-Caballero María Elvira , Tovar Clara A. , Gómez-Guillén María Carmen , Montero Pilar , Prentice Carlos
Published: October 2021

The aim of the present work was to fortify yogurt by adding a stripped weakfish (Cynoscion guatucupa) protein hydrolysate obtained with the enzyme Protamex and microencapsulated by spray drying, using maltodextrin (MD) as wall material. The effects on the physicochemical properties, syneresis, texture, viscoelasticity, antioxidant and ACE inhibitory activities of yogurt after 1 and 7 days of storage were evaluated. In addition, microbiological and sensory analyses were performed. Four yogurt formulations were prepared: control yogurt (without additives, YC), yogurt with MD (2.1%, YMD), with the free hydrolysate (1.4%, YH) and the microencapsulated hydrolysate (3.5%, YHEn). Yogurts to which free and microencapsulated hydrolysates were added presented similar characteristics, such as a slight reduction in pH and increased acidity, with a greater tendency to present a yellow color compared with the control yogurt. Moreover, they showed less syneresis, the lowest value being that of YHEn, which also showed a slight increase in cohesiveness and greater rheological stability after one week of storage. All yogurts showed high counts of the microorganisms used as starters. The hydrolysate presence in both forms resulted in yogurts with antioxidant activity and potent ACE-inhibitory activity, which were maintained after 7 days of storage. The incorporation of the hydrolysate in the microencapsulated form presented greater advantages than the direct incorporation, since encapsulation masked the fishy flavor of the hydrolysate, resulting in stable and sensorily acceptable yogurts with antioxidant and ACE inhibitory activities.
Iron-Regulated Reactive Oxygen Species Production and Programmed Cell Death in Chronic Obstructive Pulmonary Disease
Authors: Mizumura Kenji , Gon Yasuhiro
Published: October 2021

Chronic obstructive pulmonary disease (COPD) is characterized by persistent respiratory symptoms and airflow limitation. However, the pathogenesis of COPD remains unclear. Currently, it is known to involve the loss of alveolar surface area (emphysema) and airway inflammation (bronchitis), primarily due to exposure to cigarette smoke (CS). CS causes epithelial cell death, resulting in pulmonary emphysema. Moreover, CS induces iron accumulation in the mitochondria and cytosol, resulting in programmed cell death. Although apoptosis has long been investigated as the sole form of programmed cell death in COPD, accumulating evidence indicates that a regulated form of necrosis, called necroptosis, and a unique iron-dependent form of non-apoptotic cell death, called ferroptosis, is implicated in the pathogenesis of COPD. Iron metabolism plays a key role in producing reactive oxygen species (ROS), including mitochondrial ROS and lipid peroxidation end-products, and activating both necroptosis and ferroptosis. This review outlines recent studies exploring CS-mediated iron metabolism and ROS production, along with the regulation of programmed cell death in COPD. Elucidating the mechanisms of these pathways may provide novel therapeutic targets for COPD.
Localized Surface Plasmon Fields Manipulation on Nanostructures Using Wavelength Shifting
Authors: Song Hyerin , Ahn Heesang , Kim Taeyeon , Choi Jong-ryul , Kim Kyujung
Published: October 2021

Metallic nanowires have been utilized as a platform for propagating surface plasmon (SPs) fields. To be exploited for applications such as plasmonic circuits, manipulation of localized field propagating pattern is also important. In this study, we calculated the field distributions of localized surface plasmons (LSPs) on the specifically shaped nanostructures and explored the feasibility of manipulating LSP fields. Specifically, plasmonic fields were calculated at different wavelengths for a nanoscale rod array (I-shaped), an array connected with two nanoscale rods at right angles (T-shaped), and an array with three nanoscale rods at 120° to each other (Y-shaped). Three different types of nanostructures are suggested to manipulate the positions of LSP fields collaborating with adjustment of wavelength, polarization, and incident orientation of light source. The results of this study are important not only for the understanding of the wavelength-dependent surface plasmon field localization mechanism but also for the applicability of swept source-based plasmonic techniques or designing a plasmonic circuit.
Low Thermal Expansion Machine Frame Designs Using Lattice Structures
Authors: Juasiripukdee Poom , Maskery Ian , Ashcroft Ian , Leach Richard
Published: October 2021

In this work, we investigated tessellating cellular (or lattice) structures for use in a low thermal expansion machine frame. We proposed a concept for a lattice structure with tailorable effective coefficient of thermal expansion (CTE). The design is an assembly of two parts: a lattice outer part and a cylindrical inner part, which are made of homogenous materials with different positive CTEs. Several lattice design variations were investigated and their thermal and mechanical performance analysed using a finite element method. Our numerical models showed that a lattice design using Nylon 12 and ultra-high molecular weight polyethylene could yield an effective in-plane CTE of 1 × 10−9 K−1 (cf. 109 × 10−6 K−1 for solid Nylon 12). This paper showed that the combination of design optimisation and additive manufacturing can be used to achieve low CTE structures and, therefore, low thermal expansion machine frames of a few tens of centimetres in height.
A Nitrocarbazole as a New Microtubule-Targeting Agent in Breast Cancer Treatment
Authors: Sinicropi Maria Stefania , Tavani Cinzia , Rosano Camillo , Ceramella Jessica , Iacopetta Domenico , Barbarossa Alexia , Bianchi Lara , Benzi Alice , Maccagno Massimo , Ponassi Marco , Spinelli Domenico , Petrillo Giovanni
Published: October 2021

Breast cancer is still considered a high-incidence disease, and numerous are the research efforts for the development of new useful and effective therapies. Among anticancer drugs, carbazole compounds are largely studied for their anticancer properties and their ability to interfere with specific targets, such as microtubule components. The latter are involved in vital cellular functions, and the perturbation of their dynamics leads to cell cycle arrest and subsequent apoptosis. In this context, we report the anticancer activity of a series of carbazole analogues 1–8. Among them, 2-nitrocarbazole 1 exhibited the best cytotoxic profile, showing good anticancer activity against two breast cancer cell lines, namely MCF-7 and MDA-MB-231, with IC50 values of 7 ± 1.0 and 11.6 ± 0.8 μM, respectively. Furthermore, compound 1 did not interfere with the growth of the normal cell line MCF-10A, contrarily to Ellipticine, a well-known carbazole derivative used as a reference molecule. Finally, in vitro immunofluorescence analysis and in silico studies allowed us to demonstrate the ability of compound 1 to interfere with tubulin organization, similarly to vinblastine: a feature that results in triggering MCF-7 cell death by apoptosis, as demonstrated using a TUNEL assay.
The Nasal Epithelium as a Route of Infection and Clinical Signs Changes, in Rainbow Trout (Oncorhynchus mykiss) Fingerlings Infected with Aeromonas spp
Authors: Gómez de Anda Fabián Ricardo , Vega-Sánchez Vicente , Reyes-Rodríguez Nydia Edith , Martínez-Juárez Víctor Manuel , Ángeles-Hernández Juan Carlos , Acosta-Rodríguez Ismael , Campos-Montiel Rafael German , Zepeda-Velázquez Andrea Paloma
Published: October 2021

The genus Aeromonas is a group of bacteria that is widely distributed in water bodies and belongs to the normal intestinal microbiota of aquatic and terrestrial animals. In the present work, rainbow trout fingerlings were experimentally infected by an immersion bath with different Aeromonas species. Subsequently, the behavior of the infected groups was observed and recorded. Infected fingerlings were evaluated by histopathology. The highest percentages of hyperpigmentation (18.88%) and inappetence (47.7%) were observed in fish infected with A. salmonicida, while abnormal swimming (83.33%) was recorded in fish infected with A. bestiarum. In histopathological findings, the highest percentages were observed in the olfactory epithelium (50.0%) for A. lusitana and A. salmonicida (41.1%)-infected fish. While, in the nervous system, the cerebral hemispheres (31.1%) in A. media-infected fish and the oblongata medulla (40.0%) in the A. bestiarum-infected fish presented the highest percentages. Meanwhile, A. salmonicida and A. bestiarum have the highest pathogenicity and virulence based on the histopathological findings in the olfactory epithelium and nervous system. Due to the proximity of the olfactory epithelium with the nervous tissue, it is possible that the infection generated by the Aeromonas species and the histopathological findings in the nervous tissue are reflected in different behavioral changes that suggest differences in the pathogenicity and virulence of the bacteria.
Impacts of Climate Change on Blue and Green Water Resources in the Middle and Upper Yarlung Zangbo River, China
Authors: Huo Junjun , Qu Xing , Zhu Dejun , Yuan Zhe , Tang Yuanzhi
Published: October 2021

The Yarlung Zangbo River is the largest river on the Tibetan Plateau and a major international river in South Asia. Changes in the blue and green water resources in its basin are of great importance to the surrounding local and Asian regions in the context of global warming. This research used the Soil and Water Assessment Tool model to estimate blue and green flows (BWF and GWF) and analyze the spatial-temporal distribution characteristics under different hypothetical climate change scenarios. The results show that (1) the multi-year average BWF in the middle and upper reaches of the Yarlung Zangbo River Basin is 176.2 mm, the GWF is 213.1 mm, and the difference between precipitation and total water resources is only 5.4 mm; (2) both BWF and GWF in this basin showed a slightly increasing trend from 1980 to 2010, but the distribution of subbasins from upstream to downstream is decreasing; and (3) GWF has a positive correlation with both precipitation and temperature, but BWF only increases with precipitation and decreases with increasing temperature. Moreover, the change in blue and green water resources is more sensitive to the changes in precipitation than to changes in the temperature.
Accelerated Modified Tseng’s Extragradient Method for Solving Variational Inequality Problems in Hilbert Spaces
Authors: Okeke Godwin Amechi , Abbas Mujahid , De la Sen Manuel , Iqbal Hira
Published: October 2021

The aim of this paper is to propose a new iterative algorithm to approximate the solution for a variational inequality problem in real Hilbert spaces. A strong convergence result for the above problem is established under certain mild conditions. Our proposed method requires the computation of only one projection onto the feasible set in each iteration. Some numerical examples are presented to support that our proposed method performs better than some known comparable methods for solving variational inequality problems.
Human Mitochondrial DNA: Particularities and Diseases
Authors: Habbane Mouna , Montoya Julio , Rhouda Taha , Sbaoui Yousra , Radallah Driss , Emperador Sonia
Published: October 2021

Mitochondria are the cell’s power site, transforming energy into a form that the cell can employ for necessary metabolic reactions. These organelles present their own DNA. Although it codes for a small number of genes, mutations in mtDNA are common. Molecular genetics diagnosis allows the analysis of DNA in several areas such as infectiology, oncology, human genetics and personalized medicine. Knowing that the mitochondrial DNA is subject to several mutations which have a direct impact on the metabolism of the mitochondrion leading to many diseases, it is therefore necessary to detect these mutations in the patients involved. To date numerous mitochondrial mutations have been described in humans, permitting confirmation of clinical diagnosis, in addition to a better management of the patients. Therefore, different techniques are employed to study the presence or absence of mitochondrial mutations. However, new mutations are discovered, and to determine if they are the cause of disease, different functional mitochondrial studies are undertaken using transmitochondrial cybrid cells that are constructed by fusion of platelets of the patient that presents the mutation, with rho osteosarcoma cell line. Moreover, the contribution of next generation sequencing allows sequencing of the entire human genome within a single day and should be considered in the diagnosis of mitochondrial mutations.
Transcriptional Profiling of Tumorspheres Reveals TRPM4 as a Novel Stemness Regulator in Breast Cancer
Authors: Verigos John , Kordias Dimitris , Papadaki Styliani , Magklara Angeliki
Published: October 2021

Cancer stem cells (CSCs) have been implicated in the development of chemoresistance, tumor recurrence and metastasis in breast cancer, thus emerging as a promising target for novel therapies. To identify novel stemness regulators that could potentially be targeted in luminal ER+ tumors, we performed RNA-sequencing (RNA-seq) in MCF-7 adherent monolayer cells and tumorspheres enriched in breast CSCs (bCSCs). We identified 1421 differentially expressed genes (DEGs), with 923 of them being upregulated and 498 downregulated in tumorspheres. Gene ontology and pathway enrichment analyses revealed that distinct gene networks underlie the biology of the two cell systems. We selected the transient receptor potential cation channel subfamily M member 4 (TRPM4) gene that had not been associated with cancer stemness before for further investigation. We confirmed that TRPM4 was overexpressed in tumorspheres and showed that its knock-down affected the stemness properties of bCSCs in vitro. TRPM4 inhibition revealed potential anti-tumor effects by directly targeting the bCSC subpopulation. We suggest that TRPM4 plays a key role in stemness mediation, and its inhibition may represent a novel therapeutic modality against bCSCs contributing in the improvement of breast cancer treatments.
Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation
Authors: Kim Young-Su , Asif Arun , Chethikkattuveli Salih Abdul Rahim , Lee Jae-Wook , Hyun Ki-Nam , Choi Kyung-Hyun
Published: October 2021

The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis.
Nonalcoholic Fatty Liver Disease and the Kidney: A Review
Authors: Umbro Ilaria , Baratta Francesco , Angelico Francesco , Del Ben Maria
Published: October 2021

Nonalcoholic fatty liver disease (NAFLD) is associated with several extrahepatic manifestations such as cardiovascular disease and sleep apnea. Furthermore, NAFLD is reported to be associated with an increased risk of incident chronic kidney disease (CKD). Inflammation and oxidative stress are suggested to be the key factors involved in the inflammatory mechanisms and pathways linking NAFLD to CKD and are responsible for both the pathogenesis and the progression of CKD in NAFLD patients. This review aims to provide a more comprehensive overview of the association between CKD and NAFLD, also considering the effect of increasing severity of NAFLD. A PubMed search was conducted using the terms “non-alcoholic fatty liver disease AND kidney”. In total, 537 articles were retrieved in the last five years and 12 articles were included in the qualitative analysis. Our results showed that CKD developed more frequently in NAFLD patients compared to those without NAFLD. This association persisted after adjustment for traditional risk factors and according to the severity of NAFLD. Therefore, patients with NAFLD should be considered at high risk of CKD. Intensive multidisciplinary surveillance over time is needed, where hepatologists and nephrologists must act together for better and earlier treatment of NAFLD patients.
Osteopontin Expression in Thyroid Cancer: Deciphering EMT-Related Molecular Mechanisms
Authors: Viana Bruna Prunes Pena Baroni , Gomes Amanda Vitória Pampolha , Gimba Etel Rodrigues Pereira , Ferreira Luciana Bueno
Published: October 2021

Thyroid cancer is the most common tumor arising from the endocrine system and generally presents good prognosis. However, its aggressive subtypes are related to therapeutic resistance and early metastasis. Epithelial–mesenchymal transition (EMT) and its reverse process, the mesenchymal–epithelial transition (MET), are key events mediating cancer progression, including in thyroid cancer. The matricellular protein osteopontin (OPN) has been reported as a master regulator of EMT in many tumor types. Although high OPN expression has been described and associated with important aspects of thyroid cancer progression, there is no clear evidence regarding OPN as a regulator of EMT in thyroid cancer. Thus, taking together the known roles of OPN in the modulation of EMT in cancer and the information reporting the expression of OPN in thyroid tumor progression, this review aims at summarizing and discussing data related to EMT in thyroid cancer and its putative relation to the roles of OPN in the development of thyroid cancer. These data provide new insights into the molecular mechanisms by which OPN could potentially modulate EMT in thyroid tumors, generating evidence for future studies that may contribute to new therapeutic, prognostic and/or diagnostic tools.
Short-Term Epileptiform Activity Potentiates Excitatory Synapses but Does Not Affect Intrinsic Membrane Properties of Pyramidal Neurons in the Rat Hippocampus In Vitro
Authors: Ergina Julia L. , Amakhin Dmitry V. , Postnikova Tatyana Y. , Soboleva Elena B. , Zaitsev Aleksey V.
Published: October 2021

Even brief epileptic seizures can lead to activity-dependent structural remodeling of neural circuitry. Animal models show that the functional plasticity of synapses and changes in the intrinsic excitability of neurons can be crucial for epileptogenesis. However, the exact mechanisms underlying epileptogenesis remain unclear. We induced epileptiform activity in rat hippocampal slices for 15 min using a 4-aminopyridine (4-AP) in vitro model and observed hippocampal hyperexcitability for at least 1 h. We tested several possible mechanisms of this hyperexcitability, including changes in intrinsic membrane properties of neurons and presynaptic and postsynaptic alterations. Neither input resistance nor other essential biophysical properties of hippocampal CA1 pyramidal neurons were affected by epileptiform activity. The glutamate release probability also remained unchanged, as the frequency of miniature EPSCs and the paired amplitude ratio of evoked responses did not change after epileptiform activity. However, we found an increase in the AMPA/NMDA ratio, suggesting alterations in the properties of postsynaptic glutamatergic receptors. Thus, the increase in excitability of hippocampal neural networks is realized through postsynaptic mechanisms. In contrast, the intrinsic membrane properties of neurons and the probability of glutamate release from presynaptic terminals are not affected in a 4-AP model.
Obstacle Avoidance Path Planning for Worm-like Robot Using Bézier Curve
Authors: Wang Yifan , Liu Zehao , Kandhari Akhil , Daltorio Kathryn A.
Published: October 2021

Worm-like robots have demonstrated great potential in navigating through environments requiring body shape deformation. Some examples include navigating within a network of pipes, crawling through rubble for search and rescue operations, and medical applications such as endoscopy and colonoscopy. In this work, we developed path planning optimization techniques and obstacle avoidance algorithms for the peristaltic method of locomotion of worm-like robots. Based on our previous path generation study using a modified rapidly exploring random tree (RRT), we have further introduced the Bézier curve to allow more path optimization flexibility. Using Bézier curves, the path planner can explore more areas and gain more flexibility to make the path smoother. We have calculated the obstacle avoidance limitations during turning tests for a six-segment robot with the developed path planning algorithm. Based on the results of our robot simulation, we determined a safe turning clearance distance with a six-body diameter between the robot and the obstacles. When the clearance is less than this value, additional methods such as backward locomotion may need to be applied for paths with high obstacle offset. Furthermore, for a worm-like robot, the paths of subsequent segments will be slightly different than the path of the head segment. Here, we show that as the number of segments increases, the differences between the head path and tail path increase, necessitating greater lateral clearance margins.
Parenting and Sibling Relationships in Family with Disruptive Behavior Disorders. Are Non-Clinical Siblings More Vulnerable for Emotional and Behavioral Problems?
Authors: Smorti Martina , Inguaggiato Emanuela , Vezzosi Lara , Milone Annarita
Published: October 2021

Disruptive Behavior Disorders (DBD) are the most common mental health disorders in the school-aged child population. Although harsh parenting is a key risk factor in the shaping of DBD, studies neglect the presence of siblings and differential parenting. This study aims to compare: (1) parenting style and sibling relationship in sibling dyads of clinical families, composed of a DBD child and a non-clinical sibling, with control families composed of two non-clinical siblings; (2) parenting style, sibling relationship, and emotional and behavioral problems in DBD child, non-clinical sibling, and non-clinical child of control group. Sixty-one families (composed of mother and sibling dyads), divided into clinical (n = 27) and control (n = 34) groups, completed the APQ, SRI, and CBCL questionnaires. Results indicated differential parenting in clinical families, compared to control group families, with higher negative parenting toward the DBD child than the sibling; no difference emerged in sibling relationship within sibling dyads (clinical vs. control). Finally, externalizing and internalizing problems were higher in DBD children and their siblings, compared to control, indicating DBD sibling psychopathology vulnerability. Findings suggest inclusion of siblings in the clinical assessment and rehabilitative intervention of DBD children, given that the promotion of positive parenting could improve mental health in the offspring.
Sleep and PTSD in the Military Forces: A Reciprocal Relationship and a Psychiatric Approach
Authors: Saguin Emeric , Gomez-Merino Danielle , Sauvet Fabien , Leger Damien , Chennaoui Mounir
Published: October 2021

Sleep disturbances are well-recognised symptoms of Post-Traumatic Stress Disorder (PTSD). This review updates knowledge regarding the relationship between sleep during deployment, combat-related trauma, and PTSD in military personnel, from which the importance of restorative sleep results. The description of the characteristics of sleep in military forces with the considerable roles of the operational and training contexts highlights the important consequences of degraded sleep. Indeed, a lot of data suggest a dynamic link between sleep and the onset and chronicity of PTSD. We propose a reciprocal relationship model with strategies strongly recommended or already adopted by the military to promote restorative sleep before and after combat exposure. Among the alterations in a variety of sleep architecture and sleep patterns described in PTSD, the physiological hypothesis of REM sleep fragmentation in the development of PTSD symptoms may be important because REM sleep is generally associated with emotional memory. Finally, we address clinical and research perspectives that could be used to detect or restore sleep continuity before and during military deployment to possibly alleviate nightmares and insomnia related to combat exposure and PTSD occurrence and improve our understanding of sleep in PTSD.