bluuebunny/crossref_metadata_2025 huggingface dataset:
Steroid Therapy and Steroid Response in Autoimmune Pancreatitis
Authors: Matsubayashi Hiroyuki , Ishiwatari Hirotoshi , Imai Kenichiro , Kishida Yoshihiro , Ito Sayo , Hotta Kinichi , Yabuuchi Yohei , Yoshida Masao , Kakushima Naomi , Takizawa Kohei , Kawata Noboru , Ono Hiroyuki
Published: January 2020

Autoimmune pancreatitis (AIP), a unique subtype of pancreatitis, is often accompanied by systemic inflammatory disorders. AIP is classified into two distinct subtypes on the basis of the histological subtype: immunoglobulin G4 (IgG4)-related lymphoplasmacytic sclerosing pancreatitis (type 1) and idiopathic duct-centric pancreatitis (type 2). Type 1 AIP is often accompanied by systemic lesions, biliary strictures, hepatic inflammatory pseudotumors, interstitial pneumonia and nephritis, dacryoadenitis, and sialadenitis. Type 2 AIP is associated with inflammatory bowel diseases in approximately 30% of cases. Standard therapy for AIP is oral corticosteroid administration. Steroid treatment is generally indicated for symptomatic cases and is exceptionally applied for cases with diagnostic difficulty (diagnostic steroid trial) after a negative workup for malignancy. More than 90% of patients respond to steroid treatment within 1 month, and most within 2 weeks. The steroid response can be confirmed on clinical images (computed tomography, ultrasonography, endoscopic ultrasonography, magnetic resonance imaging, and 18F-fluorodeoxyglucose-positron emission tomography). Hence, the steroid response is included as an optional diagnostic item of AIP. Steroid treatment results in normalization of serological markers, including IgG4. Short- and long-term corticosteroid treatment may induce adverse events, including chronic glycometabolism, obesity, an immunocompromised status against infection, cataracts, glaucoma, osteoporosis, and myopathy. AIP is common in old age and is often associated with diabetes mellitus (33–78%). Thus, there is an argument for corticosteroid therapy in diabetes patients with no symptoms. With low-dose steroid treatment or treatment withdrawal, there is a high incidence of AIP recurrence (24–52%). Therefore, there is a need for long-term steroid maintenance therapy and/or steroid-sparing agents (immunomodulators and rituximab). Corticosteroids play a critical role in the diagnosis and treatment of AIP.
Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation
Authors: Shin Na-Hyun , Trang Do Thi , Hong Woo-Jong , Kang Kiyoon , Chuluuntsetseg Jadamba , Moon Joon-Kwan , Yoo Yo-Han , Jung Ki-Hong , Yoo Soo-Cheul
Published: January 2020

Chlorophyll breakdown is a vital catabolic process of leaf senescence as it allows the recycling of nitrogen and other nutrients. In the present study, we isolated rice senescence-induced receptor-like kinase (OsSRLK), whose transcription was upregulated in senescing rice leaves. The detached leaves of ossrlk mutant (ossrlk) contained more green pigment than those of the wild type (WT) during dark-induced senescence (DIS). HPLC and immunoblot assay revealed that degradation of chlorophyll and photosystem II proteins was repressed in ossrlk during DIS. Furthermore, ultrastructural analysis revealed that ossrlk leaves maintained the chloroplast structure with intact grana stacks during dark incubation; however, the retained green color and preserved chloroplast structures of ossrlk did not enhance the photosynthetic competence during age-dependent senescence in autumn. In ossrlk, the panicles per plant was increased and the spikelets per panicle were reduced, resulting in similar grain productivity between WT and ossrlk. By transcriptome analysis using RNA sequencing, genes related to phytohormone, senescence, and chlorophyll biogenesis were significantly altered in ossrlk compared to those in WT during DIS. Collectively, our findings indicate that OsSRLK may degrade chlorophyll by participating in a phytohormone-mediated pathway.
PP2A Functions during Mitosis and Cytokinesis in Yeasts
Authors: Moyano-Rodriguez Yolanda , Queralt Ethel
Published: January 2020

Protein phosphorylation is a common mechanism for the regulation of cell cycle progression. The opposing functions of cell cycle kinases and phosphatases are crucial for accurate chromosome segregation and exit from mitosis. Protein phosphatases 2A are heterotrimeric complexes that play essential roles in cell growth, proliferation, and regulation of the cell cycle. Here, we review the function of the protein phosphatase 2A family as the counteracting force for the mitotic kinases. We focus on recent findings in the regulation of mitotic exit and cytokinesis by PP2A phosphatases in S. cerevisiae and other fungal species.
Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat
Authors: Chen Yanger , Mao Haotian , Wu Nan , Ma Jie , Yuan Ming , Zhang Zhongwei , Yuan Shu , Zhang Huaiyu
Published: January 2020

Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most destructive wheat disease and a major problem for the productivity of wheat in the world. To obtain a better understanding about different effects of redox homeostasis and photosystem (PS) to Pst infection in wheat, we investigated the differences in photosynthesis and the antioxidant defense system in wheat cultivar Chuanmai42 (CM42) in response to two Chinese Pst races known as CYR32 and V26. The results showed that V26-infected wheat accumulated a higher reactive oxygen species (ROS), cell death, and energy dissipation than CYR32-infected wheat when compared with the control. Furthermore, we found that the activities of three antioxidant enzymes (APX, GR, and GPX) and four resistance-related enzymes in CYR32-infected wheat were significantly higher than that in V26-infected wheat. In addition, quantitative RT-PCR indicated that the expression levels of two genes associated with resistant stripe rust in CYR32-infected wheat were clearly higher than that in V26-infected wheat. Compared with CYR32-infected wheat, lower photochemical efficiencies were observed in V26-infected wheat at the adult stage. Meanwhile, only a marked decline in D1 protein was observed in V26-infected wheat. We therefore deduced that wheat with stripe rust resistance could maintain high resistance and photosynthetic capacity by regulating the antioxidant system, disease-resistant related enzymes and genes, and the levels of PSII reaction center proteins.
Traditional and New Routes of Trophoblast Invasion and Their Implications for Pregnancy Diseases
Authors: Huppertz Berthold
Published: January 2020

Historically, invasion of placental trophoblasts was thought to be extremely specific, only invading into the connective tissues of the maternal uterus and finally reaching and transforming the uterine spiral arteries. Only recently, identification of new routes of trophoblast invasion into different structures of the maternal uterus has been achieved. Thorough morphological analysis has resulted in the identification of trophoblasts invading into glands, veins, and lymph vessels of the uterine wall. These new routes pave the way for a re-evaluation of trophoblast invasion during normal placental development. Of course, such new routes of trophoblast invasion may well be altered, especially in pregnancy pathologies such as intra-uterine growth restriction, preeclampsia, early and recurrent pregnancy loss, stillbirth, and spontaneous abortion. Maybe one or more of these pregnancy pathologies show alterations in different pathways of trophoblast invasion, and, thus, etiologies may need to be redefined, and new therapies may be developed.
A Single Cell but Many Different Transcripts: A Journey into the World of Long Non-Coding RNAs
Authors: Alessio Enrico , Bonadio Raphael Severino , Buson Lisa , Chemello Francesco , Cagnin Stefano
Published: January 2020

In late 2012 it was evidenced that most of the human genome is transcribed but only a small percentage of the transcripts are translated. This observation supported the importance of non-coding RNAs and it was confirmed in several organisms. The most abundant non-translated transcripts are long non-coding RNAs (lncRNAs). In contrast to protein-coding RNAs, they show a more cell-specific expression. To understand the function of lncRNAs, it is fundamental to investigate in which cells they are preferentially expressed and to detect their subcellular localization. Recent improvements of techniques that localize single RNA molecules in tissues like single-cell RNA sequencing and fluorescence amplification methods have given a considerable boost in the knowledge of the lncRNA functions. In recent years, single-cell transcription variability was associated with non-coding RNA expression, revealing this class of RNAs as important transcripts in the cell lineage specification. The purpose of this review is to collect updated information about lncRNA classification and new findings on their function derived from single-cell analysis. We also retained useful for all researchers to describe the methods available for single-cell analysis and the databases collecting single-cell and lncRNA data. Tables are included to schematize, describe, and compare exposed concepts.
Molecular Identification of Endophytic Fungi and Their Pathogenicity Evaluation Against Dendrobium nobile and Dendrobium officinale
Authors: Sarsaiya Surendra , Jain Archana , Jia Qi , Fan Xiaokuan , Shu Fuxing , Chen Zhongwen , Zhou Qinian , Shi Jingshan , Chen Jishuang
Published: January 2020

Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch’s postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.
Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii
Authors: Dong Zhenjie , Tian Xiubin , Ma Chao , Xia Qing , Wang Beilin , Chen Qifan , Sehgal Sunish K. , Friebe Bernd , Li Huanhuan , Liu Wenxuan
Published: January 2020

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75–0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.
GABAA Receptor Ligands Often Interact with Binding Sites in the Transmembrane Domain and in the Extracellular Domain—Can the Promiscuity Code Be Cracked?
Authors: Iorio Maria Teresa , Vogel Florian Daniel , Koniuszewski Filip , Scholze Petra , Rehman Sabah , Simeone Xenia , Schnürch Michael , Mihovilovic Marko D. , Ernst Margot
Published: January 2020

Many allosteric binding sites that modulate gamma aminobutyric acid (GABA) effects have been described in heteropentameric GABA type A (GABAA) receptors, among them sites for benzodiazepines, pyrazoloquinolinones and etomidate. Diazepam not only binds at the high affinity extracellular “canonical” site, but also at sites in the transmembrane domain. Many ligands of the benzodiazepine binding site interact also with homologous sites in the extracellular domain, among them the pyrazoloquinolinones that exert modulation at extracellular α+/β− sites. Additional interaction of this chemotype with the sites for etomidate has also been described. We have recently described a new indole-based scaffold with pharmacophore features highly similar to pyrazoloquinolinones as a novel class of GABAA receptor modulators. Contrary to what the pharmacophore overlap suggests, the ligand presented here behaves very differently from the identically substituted pyrazoloquinolinone. Structural evidence demonstrates that small changes in pharmacophore features can induce radical changes in ligand binding properties. Analysis of published data reveals that many chemotypes display a strong tendency to interact promiscuously with binding sites in the transmembrane domain and others in the extracellular domain of the same receptor. Further structural investigations of this phenomenon should enable a more targeted path to less promiscuous ligands, potentially reducing side effect liabilities.
Towards More Sustainable Pavement Management Practices Using Embedded Sensor Technologies
Authors: Manosalvas-Paredes Mario , Roberts Ronald , Barriera Maria , Mantalovas Konstantinos
Published: January 2020

Road agencies are constantly being placed in difficult situations when making road maintenance and rehabilitation decisions as a result of diminishing road budgets and mounting environmental concerns for any chosen strategies. This has led practitioners to seek out new alternative and innovative ways of monitoring road conditions and planning maintenance routines. This paper considers the use of innovative piezo-floating gate (PFG) sensors and conventional strain gauges to continuously monitor the pavement condition and subsequently trigger maintenance activities. These technologies can help develop optimized maintenance strategies as opposed to traditional ad-hoc approaches, which often lead to poor decisions for road networks. To determine the environmental friendliness of these approaches, a case study was developed wherein a life cycle assessment (LCA) exercise was carried out. Observations from accelerated pavement testing over a period of three months were used to develop optimized maintenance plans. A base case is used as a guide for comparison to the optimized systems to establish the environmental impacts of changing the maintenance workflows with these approaches. On the basis of the results, the proposed methods have shown that they can, in fact, produce environmental benefits when integrated within the pavement management maintenance system.
Impact of Sustained Virological Response for Gastroesophageal Varices in Hepatitis-C-Virus-Related Liver Cirrhosis
Authors: Yuri Yukihisa , Nishikawa Hiroki , Enomoto Hirayuki , Yoh Kazunori , Iwata Yoshinori , Sakai Yoshiyuki , Kishino Kyohei , Ikeda Naoto , Takashima Tomoyuki , Aizawa Nobuhiro , Takata Ryo , Hasegawa Kunihiro , Ishii Noriko , Nishimura Takashi , Iijima Hiroko , Nishiguchi Shuhei
Published: January 2020

We aimed to clarify the relationship between sustained virological response (SVR) and gastroesophageal varices (GEVs) progression among hepatitis C virus (HCV)-related liver cirrhosis (LC) patients treated with interferon (IFN)-based therapies (n = 18) and direct-acting antiviral (DAA)-based therapies (n = 37), and LC patients with no SVR (n = 71) who had already developed GEVs. Factors influencing GEVs progression were also examined. During the follow-up period, GEVs progression was observed in 50 patients (39.7%). The 3-year cumulative GEVs progression rates in the DAA-SVR group, the IFN-SVR group, and the non-SVR group were 32.27%, 5.88%, and 33.76%, respectively (overall p value = 0.0108). Multivariate analysis revealed that sex (p = 0.0430), esophageal varices (EVs) F2 or more (p < 0.0001), and DAA-SVR (p = 0.0126, IFN-SVR as a reference) and non-SVR (p = 0.0012, IFN-SVR as a reference) were independent predictors for GEVs progression. The proportion of GEVs progression in patients with no or F1 EVs was significantly lower than that in patients with F2 or F3 EVs (33.9% (38/112) vs. 85.7% (12/14), p = 0.0003). In conclusion, IFN-based therapies can have a favorable impact for preventing GEVs progression in HCV-related LC patients with GEVs. Clinicians should be aware of a point of no return where SVR is no longer capable of avoiding GEVs progression.
UV-Cured Poly(Ethylene Glycol) Diacrylate/Carbon Nanostructure Thin Films. Preparation, Characterization, and Electrical Properties
Authors: Loginos Panagiotis , Patsidis Anastasios , Georgakilas Vasilios
Published: January 2020

Carbon nanoallotropes such as carbon nanotubes, graphene, and their derivatives have been combined with a plethora of polymers in the last years to develop new composite materials with interesting properties and applications. However, the area of photopolymer composites with carbon nanostructures has not been analogously explored. In the present article, we study the photopolymerization of poly(ethylene glycol)diacrylate (PEGDA) enriched with different carbon nanoallotropes like graphene, pristine and chemically modified carbon nanotubes (CNTs and fCNTs), and a hybrid of graphene and CNTs. The products were characterized by several microscopic and spectroscopic techniques and the electrical conductivity was studied as a function of the concentrations of carbon nanoallotropes. In general, stable thin films were produced with a concentration of carbon nanostructures up to 8.5%, although the addition of carbon nanostructures in PEGDA decreases the degree of photopolymerization, and PEDGA/carbon nanostructure composites showed electrical conductivity at a relatively low percentage.
Effect of Submicron Glass Fiber Modification on Mechanical Properties of Short Carbon Fiber Reinforced Polymer Composite with Different Fiber Length
Authors: Nguyen Nhan Thi Thanh , Kiyotaka Obunai , Kazuya Okubo , Toru Fujii , Ou Shibata , Hidehiko Tomokuni , Yukiko Fujita
Published: January 2020

In this research, three kinds of carbon fiber (CF) with lengths of 1, 3, and 25 mm were prepared for processing composite. The effect of submicron glass fiber addition (sGF) on mechanical properties of composites with different CF lengths was investigated and compared throughout static tests (i.e., bending, tensile, and impact), as well as the tension-tension fatigue test. The strengths of composites increased with the increase of CF length. However, there was a significant improvement when the fiber length changed from 1 to 3 mm. The mechanical performance of 3 and 25 mm was almost the same when having an equal volume fraction, except for the impact resistance. Comparing the static strengths when varying the sGF content, an improvement of bending strength was confirmed when sGF was added into 1 mm composite due to toughened matrix. However, when longer fiber was used and fiber concentration was high, mechanical properties of composite were almost dependent on the CF. Therefore, the modification effect of matrix due to sGF addition disappeared. In contrast to the static strengths, the fatigue durability of composites increased proportionally to the content of glass fiber in the matrix, regardless to CF length.
Morphing a Stereogram into Hologram
Authors: Canessa Enrique , Tenze Livio
Published: January 2020

We developed a method to transform stereoscopic two-dimensional (2D) images into holograms via unsupervised morphing deformations between left (L) and right (R) input images. By using robust DeepFlow and light-field rendering algorithms, we established correlations between a 2D scene and its three-dimensional (3D) display on a Looking Glass HoloPlay monitor. The possibility of applying this method, together with a lookup table for multi-view glasses-free 3D streaming with a stereo webcam, was also analyzed.
A Methodological Approach for the Assessment of Potentially Buildable Land for Tax Purposes: The Italian Case Study
Authors: Battisti Fabrizio , Campo Orazio , Forte Fabiana
Published: January 2020

According to Italian legislation for a particular type of real property—lands/areas subject to buildability, but not yet currently buildable—there is a problem related to their “qualification”, or whether or not they must be considered buildable for the purposes of their recurrent taxation. These potentially buildable (POBU) areas, that were previously zoned as “agricultural”, have been rezoned as “general urban planning instruments/regulations” (the General Urban Development Plans or variances, which regulate land governance), whose approval path has yet to be concluded. Their value—the taxable base underpinning their taxation—clearly depends on their qualification (whether or not they are considered buildable). This has produced, in recent years, several disputes between owners and local governments; the law did not give univocal solutions: Today (2019), there is a conflict of case law in relation to considering these areas as being building areas, as it is not clear what estimating procedures should be used. This article is thus based on the assumption that responding to the problems connected with taxing POBU areas must be considered separately from (overcoming, in this way, conflicting case law) the “virtual” qualification of agricultural or buildable area, but must instead, and more simply, be considered as the actual condition it is found in (likelihood of having building potential in the future), and therefore its limitations (present at the time of taxation) and the time necessary for the building to actually be built and not just “potential”. The approach proposed in this article thus offers a solution to the problem that has been raised, by modifying the current de jure approach (defining the moment when the building right is manifested) towards an assessment/appraisal approach (defining the value of the potentially buildable (POBU) area, in relation to its actual conditions). To implement this approach, a methodology—proposing an upgrade of the traditional analytic procedure for the assessment of transformation value has been structured in a way such that consideration may be made of the components characterizing the potentially buildable areas by means of appropriate assessment parameters that go towards forming these areas’ value: These are the market value discount rate of the POBU area in relation to the uncertainty and risk of reaching effective and concrete buildability, and the estimated time needed to complete the procedural path for making the area actually buildable.
An Approach to Studying the Sociolinguistic Integration of Romanian Immigrants Residing in the Community of Madrid
Authors: Pérez Cantador Yara
Published: January 2020

Grounded in different theoretical approaches of sociolinguistics, this study aims to examine the sociolinguistic integration process of the Romanian immigrant population that resides in Madrid. Semi-guided interviews are carried out with Romanian immigrants in order to obtain qualitative information regarding different aspects of the sociolinguistic integration of the immigrant population; additionally, questionnaires are administered to the same informants, with the objective of obtaining quantitative information on attitudes. Furthermore, questionnaires on attitudes were administered to 1534 informants of Spanish origin who reside in Madrid to analyze their attitudes with regard to the Romanian immigrant population living in Madrid. The analysis performed thus far indicates that the Romanian informants seem to have a good attitude toward the speech of Madrid and, in general, seem highly predisposed to integrating into their host community. The most significant conclusions drawn from the preliminary phase of analysis presented here are that although the Romanian informants perceive their integration positively, the host community does not perceive it in the same way.
Removing Fractured Endodontic NiTi Instruments with a Tube Technique: Influence of Pre-Treatment with Various Agents on Adhesive Forces In Vitro
Authors: Bürklein Sebastian , Donnermeyer David , Wefelmeier Michael , Schäfer Edgar , Urban Kent
Published: January 2020

The aim of the study was to evaluate the adhesive forces for removing iatrogenically fractured endodontic nickel-titanium instruments using a modified tube technique with various pre-treatment agents in combination with a light-curing composite. 120 Nickel-Titanium-Mtwo instruments were cut at its parallel shaft and fixed in a vise with an overlap of 2 mm. The surfaces were treated with different agents: A) GC Metalprimer; B) Prime and Bond active; C) NaOCl (3%); D) citric acid (15%); E) phosphoric acid (37%) and group (F) was not pretreated (control). One end of a matching microtube, filled with light-curing composite, was placed over the instrument and a transmitting glass fiber inserted from the opposite side guaranteed polymerization. Pull-out tests (1 mm/min) were performed and failure load was measured digitally. Data were statistically analyzed using the ANOVA and Student–Newman–Keuls tests. Interfaces were subjected to SEM analysis. Prime and Bond active created significant higher pull-out values (mean 30.5 N) compared to all other groups (p < 0.001) and Metalprimer (18.5 N) was significantly superior to the untreated (12.6 N) and NaOCl (11.7 N) group (p < 0.05). No significant differences were obtained between the other groups (p > 0.05). Thus, adhesives improved bonding to fractured NiTi instruments.
Low Temperature Joining and High Temperature Application of Segmented Half Heusler/Skutterudite Thermoelectric Joints
Authors: Wang Weian , Li Xiaoya , Gu Ming , Xing Yunfei , Bao Yefeng
Published: January 2020

A low temperature joining process has been developed to fabricate segmented half Heusler/skutterudite thermoelectric joints, and high temperature service behavior of the joints has been studied. The microstructure and electrical resistance across the joint before and after aging were investigated. The joint is well bonded and no cracks appear at the interfaces of the joint before and after aging, which can attribute to the formation of high melting point intermetallic compounds. The electrical resistance crosses the bonding layer smoothly and the contact resistance is low. These results show the process is effective, and promising for preparation of segmented thermoelectric devices.
Study of the Fatigue Crack Growth in Long-Term Operated Mild Steel under Mixed-Mode (I + II, I + III) Loading Conditions
Authors: Lesiuk Grzegorz , Smolnicki Michał , Rozumek Dariusz , Krechkovska Halyna , Student Oleksandra , Correia José , Mech Rafał , De Jesus Abílio
Published: January 2020

The paper presents an analysis of mixed-mode fatigue crack growth in bridge steel after 100-years operating time. Experiments were carried out under mode I + II configuration on Compact Tension Shear (CTS) specimens and mode I + III on rectangular specimens with lateral stress concentrator under bending and torsion loading type. Due to the lack of accurate Stress Intensity Factor (SIF) solutions, the crack path was modelled with the finite element method according to its experimental observation. As a result, the Kinetic Fatigue Fracture Diagrams (KFFD) were constructed. Due to the change in the tendency of higher fatigue crack growth rates from KI towards KIII dominance for the samples subjected to bending and torsion, it was decided to analyze this phenomenon in detail using electron-scanning microscopy. The fractographic analysis was carried out for specimens subjected to I + III crack loading mode. The mechanism of crack growth in old bridge steel at complex loads was determined and analyzed.
Effect of Hydrogen Electrosorption on Mechanical and Electronic Properties of Pd80Rh20 Alloy
Authors: Losiewicz Bozena , Kubisztal Julian , Osak Patrycja , Starczewska Oliwia
Published: January 2020

The interaction of hydrogen with Pt-group metals and alloys is at the center of research in the fields of electrochemistry, electrocatalysis, hydrogen technologies and fuel cells developed under the Hydrogen Economy. In this work, the material under study was Pd80Rh20 alloy (50 μm foil) subjected to hydrogen electrosorption at potentials corresponding to formation of α, α-β and β phase in 0.1 M H2SO4 at 25 °C. The total amount of hydrogen adsorbed at the surface and absorbed in octahedral interstitial positions of fcc Pd80Rh20 alloy, was determined from the oxidation charges. The H/(Pd+Rh) was 0.002, 0.4 and 0.8 for α, α-β, and β Pd80Rh20H, respectively. Microindentation hardness testing and nanoindentation showed weakening of mechanical properties of the Pd80Rh20 alloy after hydrogen electrosorption due to internal stresses. Decrease of work function with increasing amount of hydrogen absorbed occurred due to the surface roughness changes and the presence of electropositive hydrogen atoms absorbed in the crystal lattice responsible for the dipole interaction. The detailed mechanism of hydrogen absorption/diffusion in the Pd80Rh20 alloy structure is discussed. The obtained results give a new insight into the relationship between the amount of absorbed hydrogen and mechanical and electronic properties of the Pd80Rh20 alloy at the micro- and nanoscale.
Memristive and CMOS Devices for Neuromorphic Computing
Authors: Milo Valerio , Malavena Gerardo , Monzio Compagnoni Christian , Ielmini Daniele
Published: January 2020

Neuromorphic computing has emerged as one of the most promising paradigms to overcome the limitations of von Neumann architecture of conventional digital processors. The aim of neuromorphic computing is to faithfully reproduce the computing processes in the human brain, thus paralleling its outstanding energy efficiency and compactness. Toward this goal, however, some major challenges have to be faced. Since the brain processes information by high-density neural networks with ultra-low power consumption, novel device concepts combining high scalability, low-power operation, and advanced computing functionality must be developed. This work provides an overview of the most promising device concepts in neuromorphic computing including complementary metal-oxide semiconductor (CMOS) and memristive technologies. First, the physics and operation of CMOS-based floating-gate memory devices in artificial neural networks will be addressed. Then, several memristive concepts will be reviewed and discussed for applications in deep neural network and spiking neural network architectures. Finally, the main technology challenges and perspectives of neuromorphic computing will be discussed.
The Influence of the Strain and Stress Gradient in Determining Strain Fatigue Characteristics for Oscillatory Bending
Authors: Kurek Andrzej , Koziarska Justyna , Łagoda Tadeusz
Published: January 2020

In this study, we created a new model to determine strain fatigue characteristics obtained from a bending test. The developed model consists of comparing the stress and strain gradient surface ratio for bending and tensile elements. For model verification, seven different materials were examined based on fatigue tests we conducted, or data available in the literature: 30CrNiMo8, 10HNAP, SM45C, 16Mo3 steel, MO58 brass, and 2017A-T4 and 6082-T6 aluminum alloys. As a result, we confirmed that the proposed method can be used to determine strain fatigue characteristics that agree with the values determined on the basis of a tensile compression test.
Mechanical Characterization of Gypsum Composites Containing Inert and Insulation Materials from Construction and Demolition Waste and Further Application as A Gypsum Block
Authors: Villoria Sáez Paola , del Río Merino Mercedes , Sorrentino Marica , Porras Amores César , Santa Cruz Astorqui Jaime , Viñas Arrebola Carmen
Published: January 2020

This article analyzes the feasibility of using construction and demolition waste (expanded polystyrene, ceramic, and concrete waste) in a gypsum matrix to manufacture plaster for interior coatings or for prefabricated elements for interior partitions. To do this, several gypsum specimens were prepared (4 × 4 × 16 cm) incorporating different percentages of waste based on the weight of the gypsum (25%, 50%, and 75% of ceramic, concrete, and a mixture of both). Reference samples were also produced (without additions) to compare the results obtained. The compounds with the best performance were selected and lightened by preparing other samples in which 1/3 and 2/3 of the volume of ceramic, concrete, and mixed waste were replaced with expanded polystyrene (EPS). All samples were tested in the laboratory and the following physical and mechanical characteristics were determined: density, surface hardness, flexural strength, compressive strength, capillary water absorption, and thermal conductivity. Several applications were proposed for the selected compounds. A gypsum block with a sandwich configuration was obtained (40 × 20 × 10 cm) using the optimum compound. The block was further tested regarding its density and compression strength. A comparative analysis showed that it is possible to produce materials with a gypsum matrix by adding ceramic, concrete, and EPS waste, improving the behavior of the traditional gypsum and enabling them to be applied in various construction applications. These applications have a lower environmental impact than ordinary ones because they use less primary raw material, due to the reuse of waste.
In Vitro Evaluation of Antibacterial Properties and Smear Layer Removal/Sealer Penetration of a Novel Silver-Citrate Root Canal Irrigant
Authors: Tonini Riccardo , Giovarruscio Massimo , Gorni Fabio , Ionescu Andrei , Brambilla Eugenio , Mikhailovna Irina Makeeva , Luzi Arlinda , Maciel Pires Paula , Sauro Salvatore
Published: January 2020

This study aimed at evaluating the efficacy of a novel silver-citrate root canal irrigation solution (BioAKT) on smear layer removal, sealer penetration after root canal instrumentation and antibacterial activity. Single-root teeth were endodontically treated, sealed with an epoxi-amine resin sealer and irrigated using: Group I: 5.25% sodium hypochlorite (NaOCl); Group II: silver-citrate solution (BioAKT); Group III: phosphate buffer solution (PBS); Group IV: 17% ethylenediaminetetraacetic acid (EDTA). Smear layer removal and silver deposition at the coronal, middle and apical portion of each canal were analyzed using scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS). Sealer penetration into dentinal tubules at coronal, middle and apical portion was assessed through dye-assisted confocal microscopy (CSM). Both SEM and CSM micrographs were evaluated by two examiners (κ = 0.86), who were blind to the irrigation regimens; scores were given according to the degree of penetration of the sealer. Data analysis included Pearson’s x2 and Sidak’s multiple comparisons. Dentin discs were polished and sterilized. Enterococcus faecalis biofilms were grown using a continuous-flow bioreactor under anaerobic conditions for 72 h. Specimens were irrigated with the tested solutions, and bacterial viability was assessed using a tetrazolium salt assay (MTT). Statistical analysis included one-way ANOVA and Student’s post-hoc t-test (p < 0.05). BioAKT and EDTA were the most efficient solutions both in removing the smear layer and allowing sealer penetration. However, at the apical portion BioAKT performed significantly better compared to EDTA both in smear layer removal and sealer penetration (p < 0.05). BioAKT and NaOCl showed comparable antibacterial effect (p = 0.53). In conclusion, BioAKT represents a suitable smear layer removal agent, which allows for reliable sealer penetration at the apical portion of the root canal system and offers significant antibacterial properties.
Generalized-Fractional Tikhonov-Type Method for the Cauchy Problem of Elliptic Equation
Authors: Zhang Hongwu , Zhang Xiaoju
Published: January 2020

This article researches an ill-posed Cauchy problem of the elliptic-type equation. By placing the a-priori restriction on the exact solution we establish conditional stability. Then, based on the generalized Tikhonov and fractional Tikhonov methods, we construct a generalized-fractional Tikhonov-type regularized solution to recover the stability of the considered problem, and some sharp-type estimates of convergence for the regularized method are derived under the a-priori and a-posteriori selection rules for the regularized parameter. Finally, we verify that the proposed method is efficient and acceptable by making the corresponding numerical experiments.
Elucidation of a Unique Pattern and the Role of Carbohydrate Binding Module of an Alginate Lyase
Authors: Hu Fu , Zhu Benwei , Li Qian , Yin Heng , Sun Yun , Yao Zhong , Ming Dengming
Published: January 2020

Alginate oligosaccharides with different degrees of polymerization (DPs) possess diverse physiological activities. Therefore, in recent years, increasing attention has been drawn to the use of enzymes for the preparation of alginate oligosaccharides for food and industrial applications. Previously, we identified and characterized a novel bifunctional alginate lyase Aly7A, which can specifically release trisaccharide from three different substrate types with a unique degradation pattern. Herein, we investigated its degradation pattern by modular truncation and molecular docking. The results suggested that Aly7A adopted a unique action mode towards different substrates with the substrate chain sliding into the binding pocket of the catalytic domain to position the next trisaccharide for cleavage. Deletion of the Aly7A carbohydrate binding module (CBM) domain resulted in a complex distribution of degradation products and no preference for trisaccharide formation, indicating that the CBM may act as a “controller” during the trisaccharide release process. This study further testifies CBM as a regulator of product distribution and provides new insights into well-defined generation of alginate oligosaccharides with associated CBMs.
The Role of Non-Selective β-Blockers in Compensated Cirrhotic Patients without Major Complications
Authors: Yeh Wen-Shuo , Yang Shih-Cheng , Liang Chih-Ming , Li Yu-Chi , Tai Wei-Chen , Lee Chen-Hsiang , Yang Yao-Hsu , Hsu Chien-Ning , Tsai Tzu-Hsien , Chuah Seng-Kee , Wu Cheng-Kun
Published: January 2020

Background and Objectives: Non-selective β-blockers (NSBB) could prevent decompensation and hepatocellular carcinoma (HCC) in cirrhotic patients with clinically significant portal hypertension (CSPH), but remained uncertain for compensated cirrhotic patients without major complications. We aimed to compare the clinical outcomes between propranolol users and non-users of a CC group without major complications. Material and Methods: We conducted this population-based cohort study by using the Taiwanese Longitudinal Health Insurance Database 2000. Propranolol users (classified as cumulative defined daily dose (cDDD)) and non-PPL users were matched with a 1:1 propensity score in both cohorts. Results: This study comprised 6896 propranolol users and 6896 non-propranolol users. There was no significant impact on the development of spontaneous bacterial peritonitis between the two groups (aHR: 1.24, 95% confidence interval (CI): 0.88~1.75; p = 0.2111). Male gender, aged condition, and non-liver related diseases (peripheral vascular disease, cerebrovascular disease, dementia, pulmonary disease, and renal disease) were the independent risk factors of mortality. PPL users had significantly lower incidence of HCC development than non-users (aHR: 0.81, p = 0.0580; aHR: 0.80, p = 0.1588; and aHR: 0.49, p < 0.0001 in the groups of 1–28, 29–90, and >90 cDDD, respectively). Conclusion: The current study suggested that high cumulative doses of propranolol could decrease the risk of hepatocellular carcinoma among compensated cirrhotic patients without major complications. Further large-scale prospective studies are still required to confirm the findings in this study. Results: It remained uncertain whether non-selective β-blockers (NSBB) could prevent decompensation and hepatocellular carcinoma (HCC) in compensatory cirrhotic patients without major complications. This study aimed to compare the clinical outcomes between propranolol users and non-users of the CC group without major complications.
Estimation of the Mutagenic Potential of 8-Oxog in Nuclear Extracts of Mouse Cells Using the “Framed Mirror” Method
Authors: Gening Leonid V. , Volodin Alexandr A. , Kazachenko Konstantin Y. , Makarova Irina V. , Tarantul Vyacheslav Z.
Published: January 2020

We propose an improved earlier described “mirror” method for detecting in cell nuclear extracts mutations that arise in DNA during its replication due to the misincorporation of deoxyadenosine-5′-monophosphate (dAMP) opposite 7,8-dihydro-8-oxoguanine (8-oxoG). This method is based on the synthesis of a complementary chain (“mirror”) by nuclear extracts of different mice organs on a template containing 8-oxoG and dideoxycytidine residue (ddC) at the 3′‑end. The “mirror” was amplified by PCR using primers part of which was non-complementary to the template. It allowed obtaining the “framed mirror” products. The misincorporation of dAMP in “framed mirror” products forms an EcoRI restriction site. The restriction analysis of double-stranded “framed mirror” products allows a quantification of the mutation frequency in nuclear extracts. The data obtained show that the mutagenic potential of 8-oxoG markedly varied in different organs of adult mice and embryos.
Synthesis of the Hydrophobic Cationic Polyacrylamide (PADD) Initiated by Ultrasonic and its Flocculation and Treatment of Coal Mine Wastewater
Authors: Qi Xin , Liu Junling , Wang Cheng , Li Shiyao , Li Xiang , Liang Yicong , Sarfaraz Khan
Published: January 2020

In this study, a new type of hydrophobic cationic polyacrylamide P (AM-DMC-DABC) (PADD) was synthesized by ultrasonic (US)-initiated polymerization, which is used for the separation and removal of coal mine wastewater. The acrylamide (AM), methacryloyloxyethyl trimethyl ammonium chloride (DMC) and acryloyloxyethyl dimethylbenzyl ammonium chloride (DABC) were used as monomers to prepare). The factors that affecting the US initiated polymerization of PADD were analyzed. Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H NMR) and scanning electron microscopy (SEM) were used to characterize the chemical structure, thermal decomposition performance and surface morphology of the polymers. FT-IR and 1H NMR results showed that PADD was successfully synthesized. In addition, irregular porous surface morphology of PADD were observed by SEM analysis. Under the optimum conditions (pH = 7.0, flocculant dosage = 16.0 mg/L), the excellent flocculation performance (turbidity removal rate (TR) = 98.8%), floc size d50 = 513.467 μm, fractal dimension (Df) = 1.61, flocculation kinetics (KN0) = 27.24 × 10−3·s−1) was obtained by using high-efficiency flocculant PADD. Zeta potential analysis was used to further explore the possible flocculation mechanism of removal. The zeta potential and flocculation analytical results displayed that the flocculation removal process of coal mine wastewater mainly included hydrophobic effect, adsorption, bridging and charge neutralization, and electric patching when PADD was used. The PADD showed more excellent coal mine wastewater flocculation performance than PAD, commercial cationic polyacrylamide (CPAM) CCPAM and PAM. Thus PADD, with its good flocculation effect on coal mine wastewater under relatively wide pH range, had bright practical application value.
Endo and Exoskeleton: New Technologies on Composite Materials
Authors: Fiorillo Luca , D’Amico Cesare , Turkina Anna Yurjevna , Nicita Fabiana , Amoroso Giulia , Risitano Giacomo
Published: January 2020

The developments in the field of rehabilitation are proceeding hand in hand with those of cybernetics, with the result of obtaining increasingly performing prostheses and rehabilitations for patients. The purpose of this work is to make a brief exposition of new technologies regarding composites materials that are used in the prosthetic and rehabilitative fields. Data collection took place on scientific databases, limited to a collection of data for the last five years, in order to present news on the innovative and actual materials. The results show that some of the most commonly used last materials are glass fibers and carbon fibers. Even in the robotics field, materials of this type are beginning to be used, thanks above all to the mechanical performances they offer. Surely these new materials, which offer characteristics similar to those in humans, could favor both the rehabilitation times of our patients, and also a better quality of life.
An Evaluation of Electromagnetic Exposure While Using Ultra-High Frequency Radiofrequency Identification (UHF RFID) Guns
Authors: Zradziński Patryk , Karpowicz Jolanta , Gryz Krzysztof , Ramos Victoria
Published: January 2020

The aim is to evaluate specific absorption rate (SAR) values from exposure near handheld ultra-high frequency radiofrequency identification readers (UHF RFID guns—small electronic devices, or even portable computers with relevant accessories—emitting up to several watts of electromagnetic field (EMF) to search for RFID sensors (tags) attached to marked objects), in order to test the hypothesis that they have an insignificant environmental influence. Simulations of SAR in adult male and female models in seven exposure scenarios (gun near the head, arm, chest, hip/thigh of the operator searching for tags, or near to the chest and arm of the scanned person or a bystander). The results showed EMF exposure compliant with SAR limits for general public exposure (ICNIRP/European Recommendation 1999/519/EC) at emissions up to 1 W (reading range 3.5–11 m, depending on tag sensitivity). In the worst-case scenario, guns with a reading range exceeding 5 m (>2 W emission) may cause an SAR exceeding the general public limits in the palm of the user and the torso of the user, a bystander, or a scanned person; occupational exposure limits may be exceeded when emission >5 W. Users of electronic medical implants and pregnant women should be treated as individuals at particular risk in close proximity to guns, even at emissions of 1 W. Only UHF RFID guns emitting below 1 W may be considered as environmentally insignificant EMF sources.