bluuebunny/crossref_metadata_2025 huggingface dataset:
A Framework for Memory Efficient Context-Sensitive Program Analysis
Authors: Hedenborg Mathias , Lundberg Jonas , Löwe Welf , Trapp Martin
Published: July 2022

Static program analysis is in general more precise if it is sensitive to execution contexts (execution paths). But then it is also more expensive in terms of memory consumption. For languages with conditions and iterations, the number of contexts grows exponentially with the program size. This problem is not just a theoretical issue. Several papers evaluating inter-procedural context-sensitive data-flow analysis report severe memory problems, and the path-explosion problem is a major issue in program verification and model checking. In this paper we propose χ-terms as a means to capture and manipulate context-sensitive program information in a data-flow analysis. χ-terms are implemented as directed acyclic graphs without any redundant subgraphs. We introduce the k-approximation and the l-loop-approximation that limit the size of the context-sensitive information at the cost of analysis precision. We prove that every context-insensitive data-flow analysis has a corresponding k,l-approximated context-sensitive analysis, and that these analyses are sound and guaranteed to reach a fixed point. We also present detailed algorithms outlining a compact, redundancy-free, and DAG-based implementation of χ-terms.
Complications following surgical treatment of posterior malleolar fractures: an analysis of 300 cases
Authors: Neumann Annika Pauline , Kroker Livia , Beyer Franziska , Rammelt Stefan
Published: July 2022

Aims

The treatment of ankle fractures and fracture-dislocations involving the posterior malleolus (PM) has undergone considerable changes over the past decade. The aim of our study was to identify risk factors related to the occurrence of complications in surgically treated ankle fractures with PM involvement.

Patients and methods

We retrospectively analyzed 300 patients at a mean age of 57 years with 300 ankle fractures involving the PM treated surgically at our institution over a 12-year period. The following relevant comorbidities were noted: arterial hypertension (43.7%; n = 131), diabetes mellitus (DM) (14.0%; n = 42), thereof insulin-dependent (3.7%; n = 11), peripheral vascular disease (0.7%; n = 2), osteoporosis (12.0%; n = 36), dementia (1.0%; n = 3), and rheumatoid arthritis (2.0%; n = 6). Furthermore, nicotine consumption was recorded in 7.3% (n = 22) and alcohol abuse in 4.0% (n = 12).

Results

Complications occurred in 41 patients (13.7%). A total of 20 (6.7%) revision surgeries had to be performed. Patients with DM (p < 0.001), peripheral vascular disease (p = 0.003) and arterial hypertension (p = 0.001) had a significantly increased risk of delayed wound healing. Alcohol abuse was associated with a significantly higher overall complication rate (OR 3.40; 95% CI 0.97–11.83; p = 0.043), increased rates of wound healing problems (OR 11.32; 95% CI 1.94–65.60; p = 0.001) and malalignment requiring revision (p = 0.033). The presence of an open fracture was associated with an increased rate of infection and wound necrosis requiring revision (OR 14.25; 95% CI 2.39–84.84; p < 0.001). Multivariate analysis identified BMI (p = 0.028), insulin-dependent DM (p = 0.003), and staged fixation (p = 0.043) as independent risk factors for delayed wound healing. Compared to the traditional lateral approach, using the posterolateral approach for fibular fixation did not lead to increased complication rates.

Conclusions

Significant risk factors for the occurrence of complications following PM fracture treatment were identified. An individually tailored treatment regimen that incorporates all risk factors is important for a good outcome.
Capturing nystagmus in the emergency room: posterior circulation stroke versus acute vestibular neuritis
Authors: Nham B. , Akdal G. , Young A. S. , Özçelik P. , Tanrıverdizade T. , Ala R. T. , Bradshaw A. P. , Wang C. , Men S. , Giarola B. F. , Black D. A. , Thompson E. O. , Halmagyi G. M. , Welgampola M. S.
Published: July 2022

Objectives

To compare acute nystagmus characteristics of posterior circulation stroke (PCS) and acute vestibular neuritis (AVN) in the emergency room (ER) within 24 h of presentation.

Methods

ER-based video-nystagmography (VNG) was conducted, recording ictal nystagmus in 101 patients with PCS (on imaging) and 104 patients with AVN, diagnosed on accepted clinical and vestibular test criteria.

Results

Patients with stroke in the brainstem (38/101, affecting midbrain (n = 7), pons (n = 19), and medulla (n = 12)), cerebellum (31/101), both (15/101) or other locations (17/101) were recruited. Common PCS territories included posterior-inferior-cerebellar-artery (41/101), pontine perforators (18/101), multiple-territories (17/101) and anterior-inferior-cerebellar-artery (7/101). In PCS, 44/101 patients had no spontaneous nystagmus. Remaining PCS patients had primary position horizontal (44/101), vertical (8/101) and torsional (5/101) nystagmus. Horizontal nystagmus was 50% ipsiversive and 50% contraversive in lateralised PCS. Most PCS patients with horizontal nystagmus (28/44) had unidirectional “peripheral-appearing” nystagmus. 32/101 of PCS patients had gaze-evoked nystagmus. AVN affected the superior, inferior or both divisions of the vestibular nerve in 55/104, 4/104 and 45/104. Most (102/104) had primary position horizontal nystagmus; none had gaze-evoked nystagmus. Two inferior VN patients had contraversive torsional-downbeat nystagmus. Horizontal nystagmus with SPV ≥ 5.8 °/s separated AVN from PCS with sensitivity and specificity of 91.2% and 83.0%. Absent nystagmus, gaze-evoked nystagmus, and vertical-torsional nystagmus were highly specific for PCS (100%, 100% and 98.1%).

Conclusion

Nystagmus is often absent in PCS and always present in AVN. Unidirectional ‘peripheral-appearing’ horizontal nystagmus can be seen in PCS. ER-based VNG nystagmus assessment could provide useful diagnostic information when separating PCS from AVN.
Patient perspectives on digital healthcare technology in care and clinical trials for motor neuron disease: an international survey
Authors: Helleman Jochem , Johnson Barbara , Holdom Cory , Hobson Esther , Murray Deirdre , Steyn Frederik J. , Ngo Shyuan T. , Henders Anjali , Lokeshappa Madhura B. , Visser-Meily Johanna M. A. , van den Berg Leonard H. , Hardiman Orla , Beelen Anita , McDermott Chris , van Eijk Ruben P. A.
Published: July 2022

Introduction

To capture the patient’s attitude toward remote monitoring of motor neuron disease (MND) in care and clinical trials, and their concerns and preferences regarding the use of digital technology.

Methods

We performed an international multi-centre survey study in three MND clinics in The Netherlands, the United Kingdom, and Australia. The survey was co-developed by investigators and patients with MND, and sent to patients by e-mail or postal-mail. The main topics included: patients’ attitude towards remote care, participating in decentralized clinical trials, and preferences for and concerns with digital technology use.

Results

In total, 332 patients with MND participated. A majority of patients indicated they would be happy to self-monitor their health from home (69%), be remotely monitored by a multidisciplinary care team (75%), and would be willing to participate in clinical trials from home (65%). Patients considered respiratory function and muscle strength most valuable for home-monitoring. The majority of patients considered the use of at least three devices/apps (75%) once a week (61%) to be acceptable for home-monitoring. Fifteen percent of patients indicated they would not wish to perform home-measurements; reporting concerns about the burden and distress of home-monitoring, privacy and data security.

Conclusion

Most patients with MND exhibited a positive attitude toward the use of digital technology in both care and clinical trial settings. A subgroup of patients reported concerns with home-monitoring, which should be addressed in order to improve widespread adoption of remote digital technology in clinical MND care.
Multi-level context-driven interaction modeling for human future trajectory prediction
Authors: He Zhiquan , Sun Hao , Cao Wenming , He Henry Z.
Published: July 2022

Human trajectory prediction is a challenging task with important applications such as intelligent surveillance and autonomous driving. We recognize that pedestrians in close and distant neighborhoods have different impacts on the person’s decision of future movements. Local scene context and global scene layout also affect the movement decision differently. Existing methods have not adequately addressed these interactions between humans and the multi-level contexts occurring at different spatial and temporal scales. To this end, we propose a multi-level context-driven interaction modeling (MCDIM) method for human future trajectory learning and prediction. Specifically, we construct a multilayer graph attention network (GAT) to model the hierarchical human–human interactions. An extra set of long short-term memory networks is designed to capture the correlations of these human–human interactions at different temporal scales. To model the human–scene interactions, we explicitly extract and encode the global scene layout features and local context features in the neighborhood of the person at each time step and capture the spatial–temporal information of the interactions between human and the local scene contexts. The human–human and human–scene interactions are incorporated into the multi-level GAT-based network for accurate prediction of future trajectories. We have evaluated the method on benchmark datasets: the walking pedestrians dataset provided by ETH Zurich (ETH) and the crowd data provided by the University of Cyprus. The results demonstrate that our MCDIM method outperforms existing methods, being able to generate more accurate and plausible trajectories for pedestrians. The average performance gain is 2 and 3 percentage points in terms of the average displacement error and final displacement error, respectively.
Predictors of improvement in disease activity in first hospitalized patients with systemic lupus erythematosus: a multicenter retrospective study of a Chinese cohort
Authors: Li Mei , Liang Jun , Pan Wenyou , Liu Lin , Wu Min , Ding Fuwan , Hu Huaixia , Ding Xiang , Wei Hua , Zou Yaohong , Qian Xian , Wang Meimei , Wu Jian , Tao Juan , Tan Jun , Da Zhanyun , Zhang Miaojia , Li Jing , Feng Xuebing , Wen Lihui , Zhang Huayong , Sun Lingyun
Published: July 2022

Objectives

To analyze the relative factors of improvement in disease activity (IDA) after first hospitalized treatment based on the systemic lupus erythematosus disease activity index (SLEDAI).

Methods

A total of 1069 adult systemic lupus erythematosus (SLE) patients who were hospitalized for the first time in 26 hospitals in Jiangsu Province from 1999 to 2009 were retrospectively analyzed. SLEDAI decrease ≥ 4 during hospitalization was identified as IDA. Relative factors of IDA were assessed by univariate and multivariate logistic regression.

Results

A total of 783 (73.2%) adult SLE patients showed IDA after the first hospitalization, while the remaining patients (n = 286) were in the non-IDA group. The IDA group had higher SLEDAI at admission; fewer patients had SLICC/ACR damage index (SDI) ≥ 1, comorbidities at admission, especially Sjögren’s syndrome, abnormal serum creatinine, and glomerular filtration rate. More patients had mucocutaneous and musculoskeletal involvements, leukopenia, increased C-reactive protein, anti-dsDNA antibody positive, and hypocomplementemia at admission and were treated with methotrexate and leflunomide during hospitalization. After multivariate logistic regression analysis, SDI ≥ 1 (P = 0.005) and combined with Sjögren’s syndrome (P < 0.001) at admission had negative association with IDA. Musculoskeletal involvement (P < 0.001), anti-dsDNA antibody positive (P = 0.012), hypocomplementemia (P = 0.001), and use of leflunomide (P = 0.030) were significantly related with IDA.

Conclusion

Organ damage or comorbidities at admission were adverse to SLE improvement. Anti-dsDNA antibody positive, hypocomplementemia, musculoskeletal involvements, and leflunomide treatment had positive association with IDA of SLE.

Key Points• Organ damage or comorbidities at admission were negatively correlated with SLE improvement.• Anti-dsDNA antibody positivity, hypocomplementemia, musculoskeletal involvements, and leflunomide treatment were positively associated with SLE improvement.
Influence of appropriate emergency department utilization and verbal communication on physicians’ (dis)satisfaction with doctor–patient interactions with special consideration of migrational backgrounds
Authors: Pötter Anna Rahel , Sauzet Odile , Borde Theda , Naghavi Baharan , Razum Oliver , Sehouli Jalid , Somasundaram Rajan , Stein Hendrike , David Matthias
Published: July 2022

In recent years, utilization of emergency departments (EDs) has increased continuously, both in Germany and internationally. Inappropriate use of EDs is believed to be partly responsible for this trend. The topic of doctor–patient interaction (DPI) has received little attention in research. However, successful DPI is not only important for adherence and treatment success, but also for the satisfaction of medical staff. This non-interventionl cross-sectional study attempts to identify factors influencing physicians’ satisfaction with DPIs, with a particular focus on the appropriate utilization of EDs and verbal communication. We carried out tripartite data collection in three EDs of major referral hospitals in Berlin between July 2017 and July 2018. Migration experience, communication and language problems, level of education, and a large gap between physicians’ and patients’ perceived urgency regarding the utilization of EDs influence the quality of the doctor–patient relationships and interactions.
Social traits and credit card default: a two-stage prediction framework
Authors: Gaganis Chrysovalantis , Papadimitri Panagiota , Pasiouras Fotios , Tasiou Menelaos
Published: July 2022

Over the past years, studies shed light on how social norms and perceptions potentially affect loan repayments, with overtones for strategic default. Motivated by this strand of the literature, we incorporate collective social traits in predictive frameworks on credit card delinquencies. We propose the use of a two-stage framework. This allows us to segment a market into homogeneous sub-populations at the regional level in terms of social traits, which may proxy for perceptions and potentially unravelled behaviours. On these formed sub-populations, delinquency prediction models are fitted at a second stage. We apply this framework to a big dataset of 3.3 million credit card holders spread in 12 UK NUTS1 regions during the period 2015–2019. We find that segmentation based on social traits yields efficiency gains in terms of both computational and predictive performance compared to prediction in the overall population. This finding holds and is sustained in the long run for different sub-samples, lag counts, class imbalance correction or alternative clustering solutions based on individual and socio-economic attributes.

Graphical abstract
Development and application of equilibrium optimizer for optimal power flow calculation of power system
Authors: Houssein Essam H. , Hassan Mohamed H. , Mahdy Mohamed A. , Kamel Salah
Published: July 2022

This paper proposes an enhanced version of Equilibrium Optimizer (EO) called (EEO) for solving global optimization and the optimal power flow (OPF) problems. The proposed EEO algorithm includes a new performance reinforcement strategy with the Lévy Flight mechanism. The algorithm addresses the shortcomings of the original Equilibrium Optimizer (EO) and aims to provide better solutions (than those provided by EO) to global optimization problems, especially OPF problems. The proposed EEO efficiency was confirmed by comparing its results on the ten functions of the CEC’20 test suite, to those of other algorithms, including high-performance algorithms, i.e., CMA-ES, IMODE, AGSK and LSHADE_cnEpSin. Moreover, the statistical significance of these results was validated by the Wilcoxon’s rank-sum test. After that, the proposed EEO was applied to solve the the OPF problem. The OPF is formulated as a nonlinear optimization problem with conflicting objectives and subjected to both equality and inequality constraints. The performance of this technique is deliberated and evaluated on the standard IEEE 30-bus test system for different objectives. The obtained results of the proposed EEO algorithm is compared to the original EO algorithm and those obtained using other techniques mentioned in the literature. These Simulation results revealed that the proposed algorithm provides better optimized solutions than 20 published methods and results as well as the original EO algorithm. The EEO superiority was demonstrated through six different cases, that involved the minimization of different objectives: fuel cost, fuel cost with valve-point loading effect, emission, total active power losses, voltage deviation, and voltage instability. Also, the comparison results indicate that EEO algorithm can provide a robust, high-quality feasible solutions for different OPF problems.
Improving citizen science data for long-term monitoring of plant species in the Netherlands
Authors: van Strien Arco J. , van Zweden Jelle S. , Sparrius Laurens B. , Odé Baudewijn
Published: July 2022

In 2012, a new volunteer-based recording scheme for vascular plants was launched in the Netherlands. Its purpose is to track the changes in the number of occupied 1-km grid cells for as many native plant species as possible between survey rounds of 8 years. We did not prescribe a strict field protocol to minimize variation in observer effort, but instead chose to statistically correct for this variation with occupancy models. These models require replicated visits to a grid cell per season, which was implemented by having two independent observers survey grid cells and record all plant species observed. Now that a first survey round has ended (2012–2019), we evaluate our approach, i.e. we tested whether the scheme has the potential to produce proper trend estimates. The number of occupied grid cells in the first round was estimated per species, using an occupancy model with day of year, visit duration and observer experience as covariates for detection. The detection probability, which was 0.43 on average, strongly depended on visit duration and day of year. It was possible to estimate the number of occupied grid cells quite precisely for several hundreds of species, such that the statistical power is expected to be high enough to detect changes of 10% between survey rounds. For rare species, however, the power to detect changes is expected to be quite low. We conclude that the approach works well, but further improvements are suggested.
Inconsistencies between Subjective Reports of Cognitive Difficulties and Performance on Cognitive Tests are Associated with Elevated Internalising and Externalising Symptoms in Children with Learning-related Problems
Authors: Williams Kira L. , Holmes Joni , Farina Francesca , Vedechkina Maria ,   The CALM team, Bennett Marc P.
Published: July 2022

Children with learning difficulties are commonly assumed to have underlying cognitive deficits by health and educational professionals. However, not all children referred for psycho-educational assessment will be found to have deficits when their abilities are measured by performance on cognitive tasks. The primary aim of this study was to estimate the prevalence of this inconsistent cognitive profile (ICP) in a transdiagnostic sample of children referred by health and education service providers for problems related to attention, learning and memory (N = 715). A second aim was to explore whether elevated mental health problems were associated with ICPs. Findings suggest that approximately half of this sample could be characterised as having an ICP. Cognitive difficulties, whether identified by parent ratings or task performance, were associated with elevated internalising and externalising difficulties. Crucially, a larger discrepancy between a parent’s actual ratings of a child’s cognitive difficulties and the ratings that would be predicted based on the child’s performance on cognitive tasks was associated greater internalising and externalising difficulties for measures of working memory, and greater externalising difficulties for measures of attention. These findings suggest that subjective cognitive difficulties occurring in the absence of any task-based performance deficits may be a functional problem arising from mental health problems.
Critique and the Coloniality of Being: Rethinking Development Discourses of Encounter
Authors: Chandler David
Published: July 2022

Fleur Johns argues that the contraposition of a ‘bottom-up’ approach of politics of prototypical technique rather than the ‘top-down’ politics of the master plan or normative principle no longer seems as straightforwardly radical as it appeared when James C Scott posited the value of local knowledge or métis against grand plans of high modernization, just over 20 years ago. This paper seeks to follow Johns’ call, ‘to capture and probe some of the effects of sensibility, rationality or style widely reproduced in the details of development work’. It draws upon fieldwork in Nairobi to open up a discussion of a shift in sensibility from a ‘bottom-up’ or ‘postliberal’ approach to a framing of open-ended encounter. The paper critiques this imaginary of relational encounter by engaging contemporary work in critical black studies. It suggests that the problem of critique is that it reproduces the problems of governing imaginaries, continually seeking to rework the human subject via adaptive capacities, sensitivities to difference and openness to alterity, while leaving intact the coloniality of being, the antiblack world.
Implicit gender-science stereotypes and college-major intentions of italian adolescents
Authors: De Gioannis Elena
Published: July 2022

Gender stereotypes are often viewed as one of the root causes of the gender gap in STEM. According to Eccles’ model, they would indirectly influence major choices by shaping expectations of success and values attached to the viable options. However, empirical findings on the link between implicit gender-science stereotypes and college major intentions are limited. To fill this gap, the current study examines this association in a mixed-gender sample of 302 Italian high-school students. Logistic regression analysis revealed that implicit gender stereotypes were directly associated with females’ intention of majoring in STEM. Unlike previous findings, the mediation analysis could not confirm that other relevant factors, i.e., interest in the subject, performance at school, identification with the subject, and value attributed to the job’s salary and social utility, moderated this association.
Specification decomposition for reactive synthesis
Authors: Finkbeiner Bernd , Geier Gideon , Passing Noemi
Published: July 2022

Reactive synthesis is the task of automatically deriving a correct implementation from a specification. It is a promising technique for the development of verified programs and hardware. Despite recent advances in terms of algorithms and tools, however, reactive synthesis is still not practical when the specified systems reach a certain bound in size and complexity. In this paper, we present a sound and complete modular synthesis algorithm that automatically decomposes the specification into smaller subspecifications. For them, independent synthesis tasks are performed, significantly reducing the complexity of the individual tasks. Our decomposition algorithm guarantees that the subspecifications are independent in the sense that completely separate synthesis tasks can be performed for them. Moreover, the composition of the resulting implementations is guaranteed to satisfy the original specification. Our algorithm is a preprocessing technique that can be applied to a wide range of synthesis tools. We evaluate our approach with state-of-the-art synthesis tools on established benchmarks: the runtime decreases significantly when synthesizing implementations modularly.
Sedimentary conditions based on the vertical distribution of radionuclides in small dystrophic lakes: a case study of Toporowe Stawy Lakes (Tatra Mountains, Poland)
Authors: Szarłowicz Katarzyna , Stobiński Marcin , Jedrzejek Filip , Kubica Barbara
Published: July 2022

The aim of this work was to prove the use of radionuclides deposited in sediment core taken from an overgrowing dystrophic lakes surrounded by marsh-peat vegetation to estimate sedimentary conditions. Sediment core samples were taken from the Toporowe Stawy Lakes (Niżni (TSN) and Wyżni (TSW); Tatra Mountains). The sampling was done using a Limnos corer. After the physical sample preparations, gamma measurements were performed. Radiochemical analysis was applied with the aim of determining 210Pb radioactivity by means of 210Po. The mean values for TSN lake are as follows: 137Cs ~ 123 Bq∙kg−1, 40 K ~ 389 Bq∙kg−1, 228Th ~ 55 Bq∙kg−1, 226Ra ~ 86 Bq∙kg−1, 241Am ~ 5 Bq∙kg−1, and 210Pbuns ~ 180 Bq∙kg−1. For TSW lake, the radioactivity levels of 226Ra and 241Am are comparable to the TSN. The mean values of 137Cs, 40 K, and 228Th are almost twice as high as in TSN. The level of 210Pb in uppermost layer of TSN is higher than in TSW. Sediments were dated by use of 210Pb method, and the rate of sedimentation of each layer was also estimated. Basic chemometric tools were used to confirm the way of deposition of radionuclide, find the correlations between variables, and compare analyzed lakes. It was concluded that the presented type of lakes are a valuable source of information and the vertical distribution of radionuclide can be used to interpret the source of material supply and factors that influence the sedimentation process in recent 150–200 years.
Over-expression of a YUCCA-Like Gene Results in Altered Shoot and Stolon Branching and Reduced Potato Tuber Size
Authors: Roumeliotis Efstathios , Kloosterman Bjorn , Oortwijn Marian , Kohlen Wouter , Bouwmeester Harro J. , Bachem Christian W. B. , Visser Richard G. F.
Published: July 2022

Auxin is known to be involved in various developmental processes, including meristem identity, shoot branching and initiation of potato tubers. The previously identifiedStYUCCA8gene in potato that exhibits a peak in gene expression after tuber induction and prior to tuber swelling was cloned and over-expressed in order to study the effects of altered auxin content on shoot and stolon architecture and tuber development. The potato plants transformed with the 35S::StYUCCA8construct exhibited increased shoot and stolon branching, reduced leaf size, lower average tuber fresh weight and enhanced adventitious and lateral root formation. Investigation of the IAA content revealed that the concentration of auxin was not altered in the shoot apex but was significantly lower in the basal part of the stem despite the several 100-fold increase of expression of theStYUCCA8gene in three independent transgenic clones. This is the first time a potatoYUCCAgene is used in an experiment in order to identify the role of endogenous auxin biosynthesis in potato plant development. Our research helps elucidate the importance of small changes of auxin content on several developmental events of the potato plant, such as shoot, stolon and root architecture.
Customer comfort during service robot interactions
Authors: Becker Marc , Mahr Dominik , Odekerken-Schröder Gaby
Published: July 2022

Customer comfort during service interactions is essential for creating enjoyable customer experiences. However, although service robots are already being used in a number of service industries, it is currently not clear how customer comfort can be ensured during these novel types of service interactions. Based on a 2 × 2 online between-subjects design including 161 respondents using pictorial and text-based scenario descriptions, we empirically demonstrate that human-like (vs machine-like) service robots make customers feel more comfortable because they facilitate rapport building. Social presence does not underlie this relationship. Importantly, we find that these positive effects diminish in the presence of service failures.
Luminal and Glandular Epithelial Cells from the Porcine Endometrium maintain Cell Type-Specific Marker Gene Expression in Air–Liquid Interface Culture
Authors: Schmidhauser Meret , Ulbrich Susanne E. , Schoen Jennifer
Published: July 2022

Two different types of epithelial cells constitute the inner surface of the endometrium. While luminal epithelial cells line the uterine cavity and build the embryo-maternal contact zone, glandular epithelial cells form tubular glands reaching deeply into the endometrial stroma. To facilitate investigations considering the functional and molecular differences between the two populations of epithelial cells and their contribution to reproductive processes, we aimed at establishing differentiatedin vitromodels of both the luminal and the glandular epithelium of the porcine endometrium using an air–liquid interface (ALI) approach. We first tested if porcine luminal endometrium epithelial cells (PEEC-L) reproducibly form differentiated epithelial monolayers under ALI conditions by monitoring the morphology and the trans-epithelial electrical resistance (TEER). Subsequently, luminal (PEEC-L) and glandular epithelial cells (PEEC-G) were consecutively isolated from the endometrium of the uterine horn. Both cell types were characterized by marker gene expression analysis immediately after isolation. Cells were separately grown at the ALI and assessed by means of histomorphometry, TEER, and marker gene expression after 3 weeks of culture. PEEC-L and PEEC-G formed polarized monolayers of differentiated epithelial cells with a moderate TEER andin vivo-like morphology at the ALI. They exhibited distinct patterns of functional and cell type-specific marker gene expression after isolation and largely maintained these patterns during the culture period. The here presented cell culture procedure for PEEC-L and -G offers new opportunities to study the impact of embryonic signals, endocrine effectors, and reproductive toxins on both porcine endometrial epithelial cell types under standardizedin vitroconditions.

Graphical Abstract

Created withBioRender.com.
On Rectifiable Measures in Carnot Groups: Existence of Density
Authors: Antonelli Gioacchino , Merlo Andrea
Published: July 2022

In this paper, we start a detailed study of a new notion of rectifiability in Carnot groups: we say that a Radon measure is$${\mathscr {P}}_h$$Ph-rectifiable, for$$h\in {\mathbb {N}}$$h∈N, if it has positiveh-lower density and finiteh-upper density almost everywhere, and, at almost every point, it admits a unique tangent measure up to multiples. First, we compare$${\mathscr {P}}_h$$Ph-rectifiability with other notions of rectifiability previously known in the literature in the setting of Carnot groups, and we prove that it is strictly weaker than them. Second, we prove several structure properties of$${\mathscr {P}}_h$$Ph-rectifiable measures. Namely, we prove that the support of a$${\mathscr {P}}_h$$Ph-rectifiable measure is almost everywhere covered by sets satisfying a cone-like property, and in the particular case of$${\mathscr {P}}_h$$Ph-rectifiable measures with complemented tangents, we show that they are supported on the union of intrinsically Lipschitz and differentiable graphs. Such a covering property is used to prove the main result of this paper: we show that a$${\mathscr {P}}_h$$Ph-rectifiable measure has almost everywhere positive and finiteh-density whenever the tangents admit at least one complementary subgroup.
Decline of Lanna ceramic group production in northern Thailand (Ban Bo Suak site) confined by radiocarbon and luminescence dating
Authors: Srisunthon Prapawadee , Mueller Daniela , Preusser Frank
Published: July 2022

The Lanna group is a traditional ceramic production style from Southeast Asia, with several kiln sites found distributed all over present northern Thailand. However, its origin and development are considered controversial and chronological constraints are scarce. Applied here are radiocarbon dating to charcoal remains and luminescence dating to ceramics, kiln wall material, and fluvial sediments from the Ban Bo Suak archaeological site near Nan, northern Thailand. The site has been suspected to have been abandoned due to destruction by a flood. Unexpectedly, the ceramic samples lack proper thermoluminescence signal properties and only two samples could be dated using optically stimulated luminescence (OSL). These ages in combination with published radiocarbon ages point towards a ceramic production around AD 1700. The kiln wall material and fluvial sediments reveal evidence for partial resetting of the OSL signal, which is unexpected for heated material. Supported by some radiocarbon ages, the OSL ages imply a temporal connection between the last use of the kilns and the flood deposits, during the fifteenth century AD. Besides general methodological considerations, the data reported here indicates that while a flood apparently dismantled several kiln sites, this did not stop the production of Lanna style ceramics in the region.
Complete remission of recurrent multiple insulin-producing neuroendocrine tumors of the pancreas with somatostatin analogs: a case report and literature review
Authors: Tartaglia Andreas , Busonero Giulia , Gagliardi Lorenza , Boddi Valentina , Pieri Federica , Nizzoli Maurizio
Published: July 2022

Hyperinsulinemic hypoglycemia is most commonly caused by a single, sporadic insulinoma. Multicentric insulinoma disease (insulinomatosis) as well as metachronous neuroendocrine tumors of the pancreas, known also as neuroendocrine adenomatosis, represent a very rare condition, if not associated with multiple endocrine neoplasia type 1 syndrome (MEN1) or Von Hippel Lindau disease. We report a 9-year follow-up of a 41-year-old woman, initially presenting with hypoglycemic syndrome caused by two insulin-producing tumors, who underwent subtotal pancreasectomy in 2012, with histology compatible with multiple small neuroendocrine tumors. An approximately 1-cm insulin-producing tumor recurred at subsequent biochemical and radiological follow-up, and was cured with the somatostatin analog octreotide as a single treatment, until remission of symptoms and complete regression of the pancreatic lesion achieved after only 16 months of treatment. The possible mechanisms for these findings are discussed and the literature is briefly reviewed.
Analyzing voter behavior on social media during the 2020 US presidential election campaign
Authors: Belcastro Loris , Branda Francesco , Cantini Riccardo , Marozzo Fabrizio , Talia Domenico , Trunfio Paolo
Published: July 2022

Every day millions of people use social media platforms by generating a very large amount of opinion-rich data, which can be exploited to extract valuable information about human dynamics and behaviors. In this context, the present manuscript provides a precise view of the 2020 US presidential election by jointly applying topic discovery, opinion mining, and emotion analysis techniques on social media data. In particular, we exploited a clustering-based technique for extracting the main discussion topics and monitoring their weekly impact on social media conversation. Afterward, we leveraged a neural-based opinion mining technique for determining the political orientation of social media users by analyzing the posts they published. In this way, we were able to determine in the weeks preceding the Election Day which candidate or party public opinion is most in favor of. We also investigated the temporal dynamics of the online discussions, by studying how users’ publishing behavior is related to their political alignment. Finally, we combined sentiment analysis and text mining techniques to discover the relationship between the user polarity and sentiment expressed referring to the different candidates, thus modeling political support of social media users from an emotional viewpoint.
Nature, smells, and human wellbeing
Authors: Bentley Phoebe R. , Fisher Jessica C. , Dallimer Martin , Fish Robert D. , Austen Gail E. , Irvine Katherine N. , Davies Zoe G.
Published: July 2022

The link between nature and human wellbeing is well established. However, few studies go beyond considering the visual and auditory underpinnings of this relationship, even though engaging with nature is a multisensory experience. While research linking smell to wellbeing exists, it focuses predominantly on smells as a source of nuisance/offence. Smells clearly have a prominent influence, but a significant knowledge gap remains in the nexus of nature, smell, and wellbeing. Here, we examine how smells experienced in woodlands contribute to wellbeing across four seasons. We show that smells are associated with multiple wellbeing domains, both positively and negatively. They are linked to memories, and specific ecological characteristics and processes over space/time. By making the link between the spatiotemporal variability in biodiversity and wellbeing explicit, we unearth a new line of enquiry. Overall, the multisensory experience must be considered by researchers, practitioners, policy-makers and planners looking to improve wellbeing through nature.
Preliminary functional results after transanal irrigation in patients undergoing SHiP procedure for low rectal cancer
Authors: Bianco Francesco , Grassia Sebastiano , Goglia Marta , Gallo Gaetano
Published: July 2022

The short-stump and high-anastomosis pull-through procedure (SHiP) is a newly introduced technique in the treatment of rectal cancer. This procedure does not involve the creation of a diverting ostomy with great improvement of the patients’ quality of life in the post-operative period. However, functional post-operative alterations such as low anterior rectal resection syndrome (LARS) may occur. In this context, trans-anal irrigation (TAI) may represent a viable option in the treatment and management of LARS symptoms. The aim of the present study is to investigate the role of TAI in patients operated on SHiP procedure for low rectal cancer. A prospective database of 17 patients who underwent a SHiP procedure was maintained from April 2019 to December 2021. Anal continence and functional outcomes were assessed through LARS score and Cleveland Clinic Incontinence Score (CCIS), respectively. All patients with a LARS score > 21 underwent TAI in the post-operative period. LARS median value was 36 (IQR = 8) and drastically improved after TAI treatment to 3 (IQR = 3), as the CCIS at a mean follow-up of 9 months (SD ± 5.02). Good functional result was reached in 12 out of 13 patients (92%). Our study confirms that patients with severe post-operative dysfunction could benefit from the use of TAI.
EV-miRome-wide profiling uncovers miR-320c for detecting metastatic colorectal cancer and monitoring the therapeutic response
Authors: Yang Chan-Keng , Hsu Hung-Chih , Liu Yu-Hao , Tsai Wen-Sy , Ma Chung-Pei , Chen Yi-Tung , Tan Bertrand Chin-Ming , Lai Ying-Yu , Chang Ian Yi-Feng , Yang Chi , Yang Chia-Yu , Yu Jau-Song , Liu Hsuan
Published: July 2022

Purpose

Molecular composition of circulating small extracellular vesicles (EVs) does not merely reflect the cells of origin, but also is enriched in specific biomolecules directly associated with the cellular transformation. However, while most of the currently identified EV-miRs are only geared towards one-dimensional disease detection, their application for long-term tracking and treatment response monitoring has been largely elusive.

Methods

We established and optimized a rapid, sensitive and robust liquid biopsy sampling method, and further used small RNA sequencing to comprehensively catalogue EV-miRomes in association with the progression and outcome of metastatic colorectal cancer (mCRC).

Results

By cross-comparison of EV-miRomes (n = 290) from multi-stage and longitudinal cohorts, we uncovered a 15-EV-miR signature with dual detection and long-term monitoring of tumor size progression for mCRC. From this panel, EV-miR-320c was uncovered as a strong clinical marker – aside from its diagnostic power and a therapeutic monitoring performance superior to carcinoembryonic antigen (CEA), its high expression has also been linked to lower overall survival and a greater likelihood of disease recurrence. Further, integrative analyses of tissue transcriptomic and liquid biopsy implicated this 15-EV-miR signature in programming the mesenchymal–epithelial transition (MET) for distant localization of the metastasized cells and also in creating a tumor-favoring metastatic niche.

Conclusion

Our clinically-oriented delineation of the mCRC-associated circulating EV-miRomes systematically revealed the functional significance of these liquid biopsy markers and further strengthen their translational potential in mCRC therapeutic monitoring.

Graphical abstract
An integrated distribution scheduling and route planning of food cold chain with demand surge
Authors: Chen Youhua , Lan Hongjie , Wang Chuan , Jia Xiaoqiong
Published: July 2022

With the rapid development of e-commerce, customers could order online to ensure timeliness. Therefore, e-commerce enterprises need to pick and distribute customers’ orders. These two operations are interdependent. Order picking needs to consider the vehicle route planning. At the same time, the vehicle route planning is also based on the batching of orders. Considering the demand surge scenario of food cold chain, with the shortest time and lowest cost to complete all distribution tasks as the objective, this paper aims at the integrated optimization of distribution scheduling and route planning, and establishes a mixed integer programming mathematical model. Finally, we design a three-stage heuristic algorithm to solve this problem, and use the actual data to carry out numerical experiments to verify the reliability and effectiveness of the mathematical model and heuristic algorithm.
Connecting Calcium-Based Nanomaterials and Cancer: From Diagnosis to Therapy
Authors: Bai Shuang , Lan Yulu , Fu Shiying , Cheng Hongwei , Lu Zhixiang , Liu Gang
Published: July 2022

As the indispensable second cellular messenger, calcium signaling is involved in the regulation of almost all physiological processes by activating specific target proteins. The importance of calcium ions (Ca2+) makes its “Janus nature” strictly regulated by its concentration. Abnormal regulation of calcium signals may cause some diseases; however, artificial regulation of calcium homeostasis in local lesions may also play a therapeutic role. “Calcium overload,” for example, is characterized by excessive enrichment of intracellular Ca2+, which irreversibly switches calcium signaling from “positive regulation” to “reverse destruction,” leading to cell death. However, this undesirable death could be defined as “calcicoptosis” to offer a novel approach for cancer treatment. Indeed, Ca2+ is involved in various cancer diagnostic and therapeutic events, including calcium overload-induced calcium homeostasis disorder, calcium channels dysregulation, mitochondrial dysfunction, calcium-associated immunoregulation, cell/vascular/tumor calcification, and calcification-mediated CT imaging. In parallel, the development of multifunctional calcium-based nanomaterials (e.g., calcium phosphate, calcium carbonate, calcium peroxide, and hydroxyapatite) is becoming abundantly available. This review will highlight the latest insights of the calcium-based nanomaterials, explain their application, and provide novel perspective. Identifying and characterizing new patterns of calcium-dependent signaling and exploiting the disease element linkage offer additional translational opportunities for cancer theranostics.
A comparison between carbon footprint of water production facilities in the Canary Islands: groundwater resources vs. seawater desalination
Authors: Cruz-Pérez Noelia , Santamarta Juan C. , Gamallo-Paz Isabel , Rodríguez-Martín Jesica , García-Gil Alejandro
Published: July 2022

The Canary Islands have a water culture tied to the exploitation of their groundwater by means of wells and water galleries. However, the growth of tourism, the increase in the local population and the development of agriculture have led to the emergence of new ways of obtaining water, such as the desalination of seawater. The presence of these desalination plants covers the entire archipelago except for the island of La Palma, and sometimes they function as a complement to water needs, while in other cases they are the only source of drinking water available. To study the environmental impact of the production of drinking water through the exploitation of the aquifer and the desalination of seawater, the carbon footprint methodology was used following the guidelines of the GHG Protocol. The result has shown that seawater installations have the largest carbon footprint, mainly due to the high electricity consumption in the islands and the electricity mix of the archipelago which, as it does not rely entirely on renewable energy sources, increases CO2 emissions into the atmosphere due to the production of drinking water in the islands.
Integral Real-time Locomotion Mode Recognition Based on GA-CNN for Lower Limb Exoskeleton
Authors: Wang Jiaqi , Wu Dongmei , Gao Yongzhuo , Wang Xinrui , Li Xiaoqi , Xu Guoqiang , Dong Wei
Published: July 2022

The wearable lower limb exoskeleton is a typical human-in-loop human–robot coupled system, which conducts natural and close cooperation with the human by recognizing human locomotion timely. Requiring subject-specific training is the main challenge of the existing approaches, and most methods have the problem of insufficient recognition. This paper proposes an integral subject-adaptive real-time Locomotion Mode Recognition (LMR) method based on GA-CNN for a lower limb exoskeleton system. The LMR method is a combination of Convolutional Neural Networks (CNN) and Genetic Algorithm (GA)-based multi-sensor information selection. To improve network performance, the hyper-parameters are optimized by Bayesian optimization. An exoskeleton prototype system with multi-type sensors and novel sensing-shoes is used to verify the proposed method. Twelve locomotion modes, which composed an integral locomotion system for the daily application of the exoskeleton, can be recognized by the proposed method. According to a series of experiments, the recognizer shows strong comprehensive abilities including high accuracy, low delay, and sufficient adaption to different subjects.