bluuebunny/crossref_metadata_2025 huggingface dataset:
Bah Bolon River 1980-2023: The History of Pollution and River Used
Authors: Abus Abiyulail Alatas , Purba Meilisna , Lubis Tasnim
Published: March 2024

The existence of the Bah Bolon River is related to the surrounding community. This study was diachronic research on the history and dynamics of the Bah Bolon River from 1980-2023. Bah Bolon River is related to environmental, social and economic changes in terms of river pollution. This study aimed to invent information on the condition of the Bah Bolon River in Pematang Siantar City, North Sumatra in 1980-2023 with a focus on pollution of the Bah Bolon River and its use for the surrounding community. The method used was a historical method which consists of four stages, namely heuristics, criticism, interpretation, and historiography. Data collection was carried out through a literature review and in-depth interviews. Literature data was taken from official documents and articles regarding the Bah Bolon River from 1980-2023. In-depth interviews were conducted with informants who met the criteria of (1) full enculturation; (2) direct involvement; (3) unfamiliar cultural atmosphere; (4) sufficient time; and (5) non-analytic. The chronological analysis carried out includes the transformation of river flows, the use of water resources, and their impact on the lives of local communities. The results showed that there were changes in land use, social structure, and community economy over time. These findings contribute to a deep understanding of the dynamics of the Bah Bolon River in the 1980s, creating a foundation for evaluating the impact of environmental change on human life. The results of this research contribute to the understanding of environmental history, social change, and community adaptation to ecological change. The implications of this research can be used as a basis for future environmental conservation and water resource management policies while exploring the historical and heritage value of the Bah Bolon River.
РОЗВИТОК СОЦІАЛЬНОЇ ВІДПОВІДАЛЬНОСТІ ЗАКЛАДІВ ВИЩОЇ ОСВІТИ УКРАЇНИ НА ТЛІ СТРУКТУРНО-ІННОВАЦІЙНИХ ЗМІН В УМОВАХ ВІЙСЬКОВОГО КОНФЛІКТУ
Authors: Батченко Людмила
Published: March 2024

Військовий конфлікт спонукав заклади вищої освіти України адаптуватися до нових суспільних викликів та зміцнило їхню роль у соціальному відновленні. Дослідження розкриває, як університети реалізують інноваційні методи та ініціативи соціальної відповідальності, аби відповідати на потреби суспільства під час війни. Використання кейс-стаді, дослідження досвіду впровадження принципів та методів соціальної відповідальності в університетах всього світу, аналізу документів офіційних джерел інформації виявило перехід до дистанційного навчання, збільшення ініціатив соціальної відповідальності та зміцнення співпраці з громадськими організаціями та урядом. Результати підкреслюють важливість закладів вищої освіти у підтримці стійкості та відновленні суспільства, акцентуючи на необхідності інновацій та активної взаємодії з суспільними потребами. Адаптація та реагування на сучасні виклики підкреслює важливість ролі університетів у військовий час та їх потенціал як ключових агентів соціальних змін і відновлення.
Strategi Rekrutmen Dan Seleksi Dalam Meningkatkan Profesionalisme Guru MTs. Jam'iyah Mahmudiyah
Authors: Ritonga Lia Ariska , Neliwati Neliwati , Khalila Daulay Nurika
Published: March 2024

Penelitian  ini adalah Strategi Rekrutmen dan Seleksi Tenaga Pendidik dan Kependidikan di Mts, Jamâ€™iyah Mahmudiyah Tanjung Pura Langkat. Adapun tujuan dalam penelitian ini adalah untuk mengetahui Strategi Rekrutmen dan Seleksi Dalam meningkatkan Profesionalisme Guru di Mts Jamâ€™iyah Mahmudiyah  Tanjung Pura Kabupaten Langkat. Jenis penelitian ini adalah merupakan penelitian deskriftif kualitatif yaitu prosedur penelitian yang menghasilkan data deskriptif berupa kata-kata tertulis atau lisan dari orang-orang dan pelaku yang diamati, diarahkan dari latar belakang individu secara utuh (holistic) tanpa mengisolasikan individu dan organisasinya dalam variable tetapi memandangnya sebagai bagian dari suatu keutuhan. Dalam teknik pengumpulan data pada penelitian ini dipergunakan berbagai teknik, yaitu wawancara, observasi dan dokumentasi. Prosedur analisis data yang digunakan dalam penelitian ini adalah analisis kualitatif. Berdasarkan temuan hasil penelitian dan pembahasan maka hasil penelitian ini dapat disimpulkan bahwa pelaksanaan rekrutmen dan seleksi tenaga pendidik dan kependidikan di Mts Jamâ€™iyah Mahmudiyah  Tanjung Pura dilaksanakan dengan beberapa tahap yaitu tahap pertama dilaksanakan sesuai dengan tahapan rekrutmen karena tahapan rekrutmen dan seleksi yang pertama adalah melakukan penentuan kebutuhan yang diperlukan untuk menjadi tenaga pendidik, kemudian mengonfirmasi adanya lowongan melalui media massa, kedua Seleksi administrasi dilakukan oleh sekolah, seleksi administrasi ini meliputi seleksi berkas-berkas lamaran yang dimasukan oleh calon tenaga pendidik dan kependidikan untuk menentukan apakah sesuai dengan persyaratan yang ditentukan, Pelaksanaan tes calon tenaga pendidik di Mts Jamâ€™iyah Mahmudiyah  Tanjung Pura meliputi tes wawancara. Namun demikian, kegiatan rekrutmen dan seleksi ini tidak selalu dilakukan pada setiap tahun pelajaran, tetapi berdasarkan kebutuhan saja
MEJORAMIENTO GENÉTICO PARTICIPATIVO EN CHAYOTE
Authors: Cadena-IñígueZ J.
Published: March 2024

Con el fin de mejorar la productividad y competitividad del cultivo y comercialización del chayote, se diseñó un programa de mejoramiento genético participativo (MGP) en dos áreas agroclimáticas de Veracruz y una en San Luis Potosí, México en el periodo de 1997-2010. Se aplicó la selección masal visual estratificada considerando nueve actividades principales de trabajo participativo que incluyeron variables agronómicas y estándares del Codex alimentario. Se obtuvieron tres variedades mejoradas las cuales fueron registradas legalmente y que responden a tres segmentos del mercado exterior. Se muestra registro de producción comercial.
RENDIMIENTO Y CALIDAD DE ACEITE DE Thymus vulgaris L., POR EFECTO DE HONGOS MICORRÍZICOS, RIZOBACTERIAS Y FITORREGULADORES
Authors: Ruiz-Posadas L.M.
Published: March 2024

El tomillo (Thymus vulgaris L.) se comercializa principalmente por sus hojas y aceite esencial, y con base en la creciente demanda de ingredientes naturales y aceites de alta calidad, se evaluó el efecto que tiene la aplicación de un fitorregulador comercial, la inoculación con rizobacterias promotoras del crecimiento vegetal (RPCV) y hongos micorrízicos arbsculares (HMA) en el rendimiento y composición del aceite esencial de tomillo, resaltando la primicia del efecto de las relaciones simbióticas para la especie, con la producción y calidad de aceite, bajo un diseño experimental completamente al azar, con 32 repeticiones para cada tratamiento. La distancia de plantación fue de 1.5X1.0 m entre hileras y plantas respectivamente. El mayor rendimiento lo presentó el tratamiento de inoculación con la bacteria P61 con 13.3 mg AE g-1 MS. Se observó que el número de componentes y el porcentaje presente en el aceite esencial son diferentes en cada tratamiento. La mayor concentración de timol se registró en el Testigo (52.55%), seguido por plantas inoculadas con P61 (51%) y los HMA Zac19+Cedro (50.66%). Para O-Cimeno el tratamiento inoculado con HMA Zac19 (21.79%) presentó la mayor concentración, seguido de HMA Zac19+Cedro y el fitorregulador Bioforte® con 20.38% y 19.37%, respectivamente. Respecto a la concentración de J-terpineno, la inoculación con el HMA Cedro y la bacteria P61 mostraron mejor respuesta con 17.9 y 17.84%, respectivamente.
REORIENTACIÓN DEL SISTEMA DE PRODUCCIÓN Y COMERCIALIZACIÓN DE CHAYOTE
Authors: Cadena-Iñiguez J.
Published: March 2024

La comercialización del chayote en México, se hace vía los intermediarios; la producción es muy baja (54 t ha-1 año-1), la asociación es prácticamente nula,no existe control sanitario, cultura empresarial, equipamiento, infraestructura y los beneficios del mercado no llegan a las familias que lo producen.
Truck Load Distribution Factors in Straight and Skewed Precast Shear-connected Adjacent Voided Slab Beam Bridges
Authors: Lad Monish
Published: March 2024

Prefabricated shear-connected voided-slab beam bridges are constructed with shear keys between precast slabs to allow them to share the applied truck load by providing transverse shear continuity along with slab torsional rigidity. Per the 2019 Canadian Highway Bridge Design Code, a simplified method of analysis of these bridges under truck loads is yet unavailable. So, a parametric study, using the grillage method of analysis, was conducted to obtain the fraction of truck load carried by each precast slab as affected by bridge span and width, truck load conditions, skew angle, and support flexibility. Results were presented in the form of moment and shear distribution factors for straight bridges and a skew correction factor for skew bridges and then compared to those found in AASHTO-LRFD Bridge Design Specifications. Based on the results, a set of empirical equations were developed for the fraction of truck loading carried by the precast slab for moment and shear calculations for straight bridges along with recommendation for the skew correction factors for skewed bridges.
Open Geodemographics and Crime: A Case Study Of Toronto
Authors: Larsen Kyle
Published: March 2024

Geodemographic segmentation systems are ubiquitous in commercial applications for marketing but academic research on geodemographics shows limited focus on crime. A criticism of geodemographic segmentation systems is their closed nature, with many systems only accessible through commercial means, making them inaccessible for general public access. Using a more open approach that provides access to geodemographic systems, methodology, and applications would increase the availability and potential use of geodemographic solutions. The research presented in this paper puts forward a geodemographic segmentation system for Toronto that uses crime data in the creation of its clusters and provides the system and the methodology open to the public. This serves the dual purpose of providing a system for others to use for their own analysis on crime within Toronto and providing the methodology for others to create their own system. This research found that crime data is well suited to a geodemographic segmentation approach. Further research can apply these open data techniques to other cities or even countries.
Older Immigrant Access to Family Physicians in the Toronto CMA: A Mixed-methods Approach
Authors: Greenbaum Meira
Published: March 2024

Ontario’s demographic structure is changing, and the senior population is expected to nearly double from 2020 to 2046 (2.6 to 4.5 million), leading to increased demand for healthcare services. Older immigrants are more likely to face spatial and aspatial barriers accessing healthcare. Spatial-quantitative analysis is used to explore the potential spatial accessibility to same-language family physicians (FP) for older Chinese immigrants living in the Toronto CMA. Enhanced 2-step floating catchment area (E2SFCA) modeling reveals areas with poor to very high accessibility. To gain a further understanding of spatial accessibility and use of care, survey data from older Chinese immigrants were analyzed to reveal individual experiences in accessing primary care prior and during the COVID pandemic. The study highlights the role of spatial access and other neighbourhood and individual characteristics in older immigrants’ access to health care services, as well as the value of integrating spatial analysis with empirical data in health care research involving older immigrants.
The Impact of Technology Medium on Learning Effectiveness: Comparison of Animated Video-based Scenario, Game Movie and Serious Game
Authors: Djafarova Naza
Published: March 2024

Many proponents of the digital game-based learning (DGBL) approach believe that learning through games can revolutionize education. Indeed, DGBL is recognized as having the potential to enhance the learning experience, but it is not well researched how it improves learning effectiveness. 

This study compares the efficacy between the game and video-based learning tools. Based on Self-Determination Theory and Flow Theory of Optimal Experience, we investigated a relationship between learning tools, motivation, flow, and learning outcomes. 

We used the experimental data collection method under controlled conditions with 340 university students. Results showed significantly higher learning outcomes for those who learned through learning tools designed using instructional design methodologies. We suggest that the application of instructional design practices matters, and well-designed game-based and videobased learning tools can be equally effective. It should not be assumed that one format of learning tool is more effective than another.
Business Improvement Areas and the Role of the Visitor Economy in the Survival, Recovery, and Progression of Main Streets in the Context of COVID-19
Authors: Francis Natasha
Published: March 2024

Business Improvement Areas (BIAs) are associations of property owners and tenants within a defined area who undertake initiatives to create successful business environments and attract people to them. This fundamental function shares similarities with the idea of a tourism destination, which manages the quality of the destination and attracts visitors to it. Despite this natural connectivity, current literature has yet to explore the relationship. BIAs may benefit from the application of a destination mindset to the BIA model to strategically attract and engage visitors, particularly as a long-term COVID-19 recovery strategy. This exploratory study involved a thematic analysis of 32 semi-structured interviews with Canadian BIA representatives. Findings demonstrate that BIAs are important actors in local visitor economy development and engagement, and that the visitor economy is important for BIAs in achieving their objectives. This thesis offers 13 recommendations for BIAs to consider to more effectively engage the visitor economy.
Keefektifan Penggunaan Model Project Based Learning dalam Pembelajaran Keterampilan Menulis Puisi Siswa
Authors: Chaerunnisa Nurul , Adam Andi , Rahayu Sri
Published: March 2024

This study aims to determine the effectiveness of using a project-based learning model on learning to write student poetry in class V SD Inpres Taeng-Taeng, Gowa Regency. This type of research is a quasi-experiment. The sample in this study was 25 students. Data collection techniques were carried out using questionnaires, writing tests, and observation sheets. The results showed that the poetry writing skills of students before the implementation of the project-based learning model were generally in the poor category and the learning outcomes on the pretest with an average value of 49.20. While the learning outcomes of students' poetry writing skills after the implementation of the project-based learning model changed to a satisfactory category and the learning outcomes of students on the posttest with an average value of 90.00 the results of descriptive analysis testing with the help of SPSS 27.0 for windows. Normality testing of pretest and post-test data has been normally distributed with sig 0.17> α = 0.05 and the results of hypothesis testing using the One sample t-test and obtained a p-value (sig. (2-tailed)) is 0.000 <0.05, so H0 is rejected and Ha is accepted, which means that the Project Based Learning model is effectively used in the poetry writing skills of fifth-grade students of SD Inpres Taeng-Taeng Gowa Regency.
Dekadensi Kesantunan Berbahasa Mahasiswa Lintas Budaya di Universitas Tribhuwana Tunggadewi Malang: Kajian Antropososiopragmatik
Authors: Kamhar Muhammad Yusi , Mulyono Mulyono , Mintowati Mintowati , Lestari Erma
Published: March 2024

This research examines the phenomenon of the decadence of language politeness by focusing on changes in politeness values ​​and the decline in communication ethics. This research is based on Brown and Levinson's theory of politeness which pays attention to the factors of social distance, social power, and threat ratings in communication, as well as anthroposociopragmatic theory to understand the influence of culture and social structure on language use. The method used is descriptive qualitative, with data collection through direct observation and analysis of student digital communication documents, especially via WhatsApp. Data analysis shows that there is a decline in politeness in language which is characterized by the use of less polite speech, both in daily communication between students and with lecturers. The research results show that the decadence of language politeness among cross-cultural students at Universitas Tribhuwana Tunggadewi Malang is influenced by various factors, including environmental influences, social media, and a lack of understanding of language politeness norms in diverse socio-cultural contexts. This study emphasizes the importance of understanding intercultural communication, cultural identity, and social interaction in overcoming the decadence of language politeness, as well as the need to integrate material on language politeness in higher education to develop effective and polite student communicative competence.
Hasil Luaran Bayi pada Ibu Hamil yang Mengalami Preeklampsia
Authors: Nurlana Zamaun  , Anna Sari Dewi  , Syamsu Rachmat Faisal , Andi Mappaware Nasruddin , Arfah Arni Isnaini , A. Madjid Djauhariah , Syarifuddin Rauf
Published: March 2024

Preeklampsia adalah sindrom klinis pada masa kehamilan setelah kehamilan 20 minggu yang ditandai dengan peningkatan tekanan darah >140/90 mmHg. Faktor resiko terjadinya preeklamsia adalah usia, nulliparitas, riwayat preeklamsia sebelumnya, riwayat penyakit hipertensi dan diabetes melitus, riwayat keluarga dan obesitas. Beberapa teori mengemukakan tentang bagaimana terjadinya preeklampsia antara lain teori genetik, teori iskemia uteroplasenter, teori kerusakan endotel pembuluh darah. Tujuan dari literature review ini bertujuan untuk mengetahui hasil luaran bayi pada ibu yang mengalami preeklampsia. Jenis penelitian ini menggunakan literature review dengan desain narrative review yaitu mencari artikel yang membahas tentang hasil luaran bayi pada ibu yang mengalami preeklampsia yang telah dipublikasikan pada jurnal dari tahun 2017-2020.  Jurnal dipilih berdasarkan kriteria inklusi dan eksklusi yang telah ditetapkan sebelumnya. Berdasarkan telaah dari beberapa jurnal, didapatkan hasil luaran bayi pada ibu yang mengalami preeklampsia adalah bayi kurang bulan kecil masa kehamilan (KMK), bayi kurang bulan sesuai masa kehamilan (SMK), dan asfiksia.
Controlling Electron Spin to Realise a Greener Future
Authors: Sewell Kevin
Published: March 2024

The Information and Communication Technology (ICT) sector is consuming an ever-increasing amount of electricity. Most electronic devices are composed of billions of transistors, which are made of materials called semiconductors. Semiconducting devices can be engineered to hold properties that suit the device’s function. One relatively unexplored property is electron spin, which, if it can be controlled, may be manipulated to increase the efficiency of semiconducting devices, particularly regarding data transfer and storage. I want to examine whether spin polarisation (the degree to which the spin of electrons aligns with a given direction) can be controlled. This is achieved by understanding the electron-scattering processes by which electrons change their spin polarisation in time. The ultimate potential impact of this research is leaner, greener telecommunications and computing.
“The whole of science is nothing more than a refinement of everyday thinking.” — Albert Einstein
Plantas alimentícias não convencionais utilizadas pelas famílias agricultoras do Núcleo Luta Camponesa da Rede Ecovida de Agroecologia, Paraná
Authors: Rauber Ana Cláudia , Leandrini Josimeire Aparecida , Franzener Gilmar
Published: March 2024

Existe uma grande diversidade de plantas nativas ou cultivadas que pode ser utilizada na alimentação eque contribuem com a soberania alimentar. Assim, esta pesquisa objetivou verificar quais plantas alimentícias não convencionais (PANC) são conhecidas e utilizadas pelas famílias agricultoras pertencentes ao Núcleo Luta Camponesa da Rede Ecovida de Agroecologia, Paraná. Para tanto, foram realizadas entrevistas semiestruturadas, caminhada etnobotânica e observação participante, em 30 unidades de produção familiar. Foram citadas 67 etnoespécies, sendo 34 nativas, 29 cultivadas e quatro naturalizadas. Constatou-se que algumas PANC foram substituídas por espécies convencionais, e que houve redução da ocorrência de algumas espécies devido à modernização agrícola. Com a transição agroecológica realizada em unidades produtivas, muitas PANC passaram a ser incorporadas ao agroecossistema. Portanto, as famílias camponesas conhecem e utilizam as PANC, e estas já fazem parte da cultura alimentar ou estão sendo conhecidas, sendo que a agroecologia tem papel fundamental no resgate destes saberes e na divulgação dos novos.
Cenários de saúde, trabalho e vida no município de Casimiro de Abreu (RJ): apontamentos para uma ação pública local voltada ao trabalho agrícola
Authors: Silva Valéria dos Santos Pinto da , De Albuquerque Campos Elida , Indio do Brasil da Costa Vanessa , De Albuquerque Melo Nogueira Fernanda , Barros Otero Ubirani , Sarpa Marcia , Casimiro de Abreu Grupo Técnico
Published: March 2024

O espaço rural é mais que a dimensão agrícola, é um sistema complexo que aproxima a agroecologia da saúde. Compartilhar uma experiência de construção coletiva para ações públicas no nível municipal é relevante. Em Casimiro de Abreu (RJ), foram realizados grupos de discussão com agricultores(as) para conhecer a relação saúde e trabalho, estimular a expressão de necessidades e a definição do modelo local.
Evaluation of Prognostic Significance of Serum Magnesium Level in Patients with Acute Myocardial Infarction
Authors: Sumaiya Binte Wahid  , Shahin Ara  , Ayub Ali  , Farzana Sharmin Payel  , Rahaman Md Arifur , Farhana Yasmin
Published: March 2024

Background: Magnesium has been considered an important factor in the pathogenesis of acute myocardial infarction and its complications. Magnesium ions are essential for the maintenance of the functional integrity of the myocardium. It also improves vascular tone, afterload and cardiac output, and decreases peripheral vascular resistance and cardiac arrhythmias. Serum magnesium concentration has great significance in the prognosis of acute myocardial infarction (MI). Aim and objective: This study aimed to assess the relation of low serum magnesium levels with post-infarction complications of acute MI.  Methods: This cross-sectional descriptive study was conducted in the Department of Pharmacology & Therapeutics, Rajshahi Medical College in collaboration with the Cardiology department between July 2019 to June 2020. Assessment and comparison of the serum magnesium level of the patients on the 5th day of post-infarction with the 1st day of admission in 50 patients of acute MI was done in this study. Results: The mean magnesium level was 2.25 ± 0.15 mg/dl in patients without any complications which was significantly higher than the patients who had multiple complications.  The level of magnesium was 1.79 ± 0.37 mg/dl in complicated cases. (P˂0.001). These observations suggest that in acute myocardial infarction, patients with low magnesium levels are more prone to develop complications. Conclusion: So it can be concluded that measurement of serum magnesium levels has prognostic significance and magnesium treatment can be implicated in patients of acute myocardial infarction with low magnesium levels.
Mediscope 2024;11(1): 27-33
EFFECTIVENESS OF ABSORPTION WELLS USING THE SUNJOTO METHOD TO CONTROL FLOODING IN THE VILLAGE OF NGRASEH BOJONEGORO
Authors: Mushthofa Mushthofa , Fadhila Fajrina Nur , Indriani Yulia , Faqih Nasyiin , Qomaruddin Mochammad
Published: March 2024

Infiltration wells are a form of hydrological infrastructure used to manage rainwater. Infiltration wells are usually built as part of a rainwater management system to soak rainwater into the ground and reduce surface waterlogging as well as slow down surface runoff and allow rainwater to seep into the ground to renew aquifers or prevent waterlogging. Maintaining groundwater levels is an important step in sustainable water resource management. The aim of this research is to plan effective infiltration wells to replace rainwater catchment areas lost due to building construction. The planning of this infiltration well uses the Sunjoto  method. From the research results, it was found that an effective infiltration well design was calculated using the Sunjoto  method. With the data, each roof area of ​​the house has an optimum number of infiltration wells with a planned infiltration well diameter of 1 m with a depth of 2.5 m with an effective depth according to the calculations is as follows: 60 m2 number of 1 unit of absorption well with an optimum depth of 2.82 m, 80 m2 number of 2 units of absorption well with an optimum depth of 3.76 m, 100 m2 number of 2 units of absorption well with an optimum depth of 4.7 m, 120 m2 number of 2 units of infiltration wells with an optimum depth of 5.64 m, 140 m2 number of 3 units of absorption wells with an optimum depth of 6.58 m, 160 m2 number of 3 units of absorption wells with an optimum depth of 7.52 m, 180 m2 number of 3 units of absorption wells with an optimum depth of 8.46 m, 200 m2 total of 4 units of infiltration wells with  an  optimum depth  of 9.4 m.
Formulasi dan Karakterisasi Nanoemulsi Ekstrak Etanol Buah Wualae (Etlingera Elatior (Jack) R.M. Smith)
Authors: Wa Ode Sitti Zubaydah  , Astrid Indalifiany  , Yamin  , Suryani  , Dian Munasari  , Muhammad Handoyo Sahumena  , Sitti Raodah Nurul Jannah
Published: March 2024

Wualae (Etlingera elatior (Jack) R.M. Smith) has a high flavonoid content, so it has the potential as an antioxidant and can be used in drug delivery systems, namely nanoemulsions. Nanoemulsions can improve absorption, help dissolve lipophilic drugs and increase bioavailability. This study aims to determine the formulation and characterization of wualae nanoemulsion. The research method used is a low-energy emulsification method. The nanoemulsion is formulated into four formulas with a concentration ratio of tween 80 and PEG 400, namely 50%: 30% (F1), 50%: 10% (F2), 10%: 30% (F3), and 10%: 10% (F4). The characterizations included organoleptic tests, nanoemulsion type tests, viscosity tests, transmittance tests, particle size tests, polydispersity index tests, potential zeta and physical tests, and centrifugation and cycling tests. The results obtained sequentially are F1 has a characteristic odour of clear yellow, type of oil-in-water nanoemulsion, 150 cPa.s, 99%, 14.56 ± 0.666 nm, 0.061 ± 0.017, 1.453 ± 1.23 mV and is stable in the test. F2 has a characteristic odour of clear yellow colour, oil-in-water nanoemulsion type, 900 cPa.s, 97.2%, 13.8 ± 0.781 nm, 0.126 ± 0.066, -5.503 ± 0.57 mV and is stable in difficult tests. F3 has a dirty white type of nanoemulsion oil in 9.5 cPa and is unstable in typical tests. F4 has a characteristic odour of cloudy white colour, oil-in-water nanoemulsion type, 6 cPa.s and is inconsistent in stability testing. Based on the data above, the best wualae fruit extract nanoemulsion formulation is formula 1.
Potensi Aktivitas Sitotoksik Belimbing Wuluh (Averrhoa bilimbi L.) Pada Sel Kanker
Authors: Utami Widyaningrum , Saragih Elisa Br. , Andini Merilla
Published: March 2024

Kemoterapi merupakan pengobatan yang efektif digunakan untuk terapi berbagai jenis sel kanker namun memiliki banyak efek samping. Belimbing wuluh (Averrhoa bilimbi L.) merupakan alternatif tanaman obat digunakan untuk pengobatan tradisional seperti antibakteri, antivirus, dan antikanker.  A. bilimbi memiliki kandungan fenolik yang tinggi yang didunga memiliki aktivitas sitotoksik. Tujuan dari review ini adalah untuk mengetahui potensi Averrhoa bilimbi L sebagai agen antikanker. Pencarian literatur dilakukan dengan melalui database online Google Scholar dan PubMed tahun 2011-2022. Senyawa aktif yang diduga memiliki efek antikanker pada belimbing wuluh yaitu squalene dan asam protocatechuic. Beberapa ekstrak etanol belimbing wuluh terbukti berpotensi sebagai agen antikanker secara in vitro dengan kategori sitotoksik sedang (IC50 = 21 µg/mL - 200 µg/mL) pada sel kanker serviks (sel HeLa), sel kanker payudara MCF-7, dan sel kanker payudara non hormonal (MDA-MB-23). Sedangkan secara in vivo, Averrhoa bilimbi L. terbukti memiliki aktivitas antikanker sebagai antilimfoma pada mencit albino Swiss yang diinduksi DAL (Dalton’s asites Lymphoma) dan potensi apoptogenik yang signifikan terhadap sel EAC (Ehrlich ascites carcinoma) tikus. Maka dari itu, kajian ini menunjukkan potensi aktivitas Averrhoa bilimbi L. sebagai antikanker.
PENINGKATAN PEMAHAMAN DAN KEWASPADAAN MASYARAKAT TERHADAP KASUS RESISTENSI ANTIBIOTIK DI SMA NEGERI 2 KENDARI
Authors: Malaka Muhammad Hajrul , Sahidin  , Sitti Raodah Nurul Jannah  , Azis Muhammad Israwan , Hamsidi Rini
Published: March 2024

Resistensi antibiotik merupakan permasalahan yang diakibatkan oleh penggunaan obat yang tidak rasional. dan menjadi tantangan terbesar di Indonesia yang merupakan negara tropis dengan prevalensi penyakit infeksi yang tinggi. Penggunaan antibiotik yang tidak rasional contohnya adalah penggunaan antibiotik tanpa resep dokter dan masih menjadi hal yang umum di masyarakat terutama di SMA Negeri 02 Kendari. Salah satu penyebab terjadinya kasus resistensi antibiotik adalah kurangnya pengetahuan masyarakat mengenai penggunaan antibiotik yang benar. Sosialisasi dilakukan dengan tujuan untuk meningkatkan pemahaman siswa dan siswi di SMA Negeri 02 Kendari mengenai antibiotik, penggunaan antibiotik secara bijak, dan meningkatkan kewaspadaan masyarakat terhadap kasus resistensi antibiotik. Dalam pelaksanaannya sosialisasi dilakukan secara tatap muka bersama guru maupun siswa dan siswi SMA Negeri 02 Kendari, serta pemberian leaflet berisi informasi tentang antibiotik. Pemberian kuisioner pre-test dan post-test ­juga dilakukan untuk mengetahui tingkat pemahaman masyarakat sebelum dan setelah sosialisasi. Hasil yang diperoleh menunjukkan nilai pre-test sebesar 6,7% untuk tingkat pengetahuan rendah, 20% untuk tingkat pengetahuan sedang dan 73,3% untuk tingkat pengetahuan tinggi, sehingga diperoleh kenaikan pemahaman siswa dan siswi dari hasil post-test. Berdasarkan hasil tersebut dapat disimpulkan bahwa sosialisasi dan pemberian informasi melalui leaflet dapat meningkatkan pemahaman masyarakat mengenai pentingnya penggunaan antibiotik secara bijak untuk mencegah kasus resistensi antibiotik.
SOSIALISASI DAN EDUKASI TENTANG PHBS DI PUSKESMAS KANDAI KECAMATAN KENDARI KOTA KENDARI SULAWESI TENGGARA
Authors: Indradewi Fery , Ruslin
Published: March 2024

PHBS adalah semua perilaku kesehatan yang dilakukan atas dasar kesadaran sehingga anggota keluarga dapat menolong dirinya sendiri di bidang kesehatan dan berperan aktif dalam kegiatan-kegiatan kesehatan di masyarakat. Sulawesi Tenggara salah satu provinsi yang masih rendah cakupan PHBSnya yaitu hanya mencakup 43,8% jika dibandingkan dengan standar cakupan Indonesia yaitu 55%. Oleh karena itu, kami melakukan sosialisasi PHBS tepatnya di Puskesmas Kandai untuk meningkatkan kepatuhan masyarakat mengenai pentingnya perilaku hidup bersih dan sehat untuk meningkatkan Kesehatan masyarakat dan mencegah kejadian penyakit menular maupun tidak menular. Metode yang digunakan pada kegiatan pengabdian ini berupa metode ceramah melalui sosialisasi pemahaman kepada masyarakat di Puskesmas Kandai mengenai Perilaku Hidup Bersih dan Sehat (PHBS). Serta pemberian informasi kepada masyarakat di Puskesmas Kandai terkait hal-hal yang berhubungan dengan Perilaku Hidup Bersih dan Sehat (PHBS) menggunakan leaflet dan video edukasi. Berdasarkan hasil penjumlahan skor jawaban responden sebelum dan sesudah pemberian informasi memiliki nilai (skor) rata-rata Baik. Sehingga dapat disimpulkan bahwa masyarakat di Kelurahan Kandai, Kecamatan Kendari, Kota Kendari Sulawesi Tenggara, memiliki kesadaran penuh mengenai pentingnya menerapkan Perilaku Hidup Bersih dan Sehat dalam kehidupan sehari-hari
Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf
Authors: Zhang Yan , Jiang Chuandong , Li Yangyang , Sun Jingguo , Chen Zhenguo , Zhang Qiang , Sun Guangwei
Published: March 2024

Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as Bacillus subtilis by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.
Molecular dynamics of the human RhD and RhAG blood group proteins
Authors: Floch Aline , Galochkina Tatiana , Pirenne France , Tournamille Christophe , de Brevern Alexandre G.
Published: March 2024

Introduction: Blood group antigens of the RH system (formerly known as “Rhesus”) play an important role in transfusion medicine because of the severe haemolytic consequences of antibodies to these antigens. No crystal structure is available for RhD proteins with its partner RhAG, and the precise stoichiometry of the trimer complex remains unknown.

Methods: To analyse their structural properties, the trimers formed by RhD and/or RhAG subunits were generated by protein modelling and molecular dynamics simulations were performed.

Results: No major differences in structural behaviour were found between trimers of different compositions. The conformation of the subunits is relatively constant during molecular dynamics simulations, except for three large disordered loops.

Discussion: This work makes it possible to propose a reasonable stoichiometry and demonstrates the potential of studying the structural behaviour of these proteins to investigate the hundreds of genetic variants relevant to transfusion medicine.
Combination of metagenomic next-generation sequencing and conventional tests unraveled pathogen profiles in infected patients undergoing allogeneic hematopoietic stem cell transplantation in Jilin Province of China
Authors: Zou Hongyan , Gao Sujun , Liu Xiaoliang , Liu Yong , Xiao Yunping , Li Ao , Jiang Yanfang
Published: March 2024

Background

Infection is the main cause of death for patients after allogeneic hematopoietic stem cell transplantation (HSCT). However, pathogen profiles still have not been reported in detail due to their heterogeneity caused by geographic region.

Objective

To evaluate the performance of metagenomic next-generation sequencing (mNGS) and summarize regional pathogen profiles of infected patients after HSCT.

Methods

From February 2021 to August 2022, 64 patients, admitted to the Department of Hematology of The First Hospital of Jilin University for HSCT and diagnosed as suspected infections, were retrospectively enrolled.

Results

A total of 38 patients were diagnosed as having infections, including bloodstream (n =17), pulmonary (n =16), central nervous system (CNS) (n =4), and chest (n =1) infections. Human betaherpesvirus 5 (CMV) was the most common pathogen in both bloodstream (n =10) and pulmonary (n =8) infections, while CNS (n =2) and chest (n =1) infections were mainly caused by Human gammaherpesvirus 4 (EBV). For bloodstream infection, Mycobacterium tuberculosis complex (n =3), Staphylococcus epidermidis (n =1), and Candida tropicalis (n =1) were also diagnosed as causative pathogens. Furthermore, mNGS combined with conventional tests can identify more causative pathogens with high sensitivity of 82.9% (95% CI 70.4-95.3%), and the total coincidence rate can reach up to 76.7% (95% CI 64.1-89.4%).

Conclusions

Our findings emphasized the importance of mNGS in diagnosing, managing, and ruling out infections, and an era of more rapid, independent, and impartial diagnosis of infections after HSCT can be expected.
Is the leptin/BMI ratio a reliable biomarker for endometriosis?
Authors: Zyguła Aleksandra , Sankiewicz Anna , Sakowicz Agata , Dobrzyńska Ewa , Dakowicz Agnieszka , Mańka Grzegorz , Kiecka Mariusz , Spaczynski Robert , Piekarski Piotr , Banaszewska Beata , Jakimiuk Artur , Issat Tadeusz , Rokita Wojciech , Młodawski Jakub , Szubert Maria , Sieroszewski Piotr , Raba Grzegorz , Szczupak Kamil , Kluza Tomasz , Kluza Marek , Pierzyński Piotr , Wojtyla Cezary , Lipa Michal , Warzecha Damian , Wielgos Miroslaw , Cendrowski Krzysztof , Gorodkiewicz Ewa , Laudanski Piotr
Published: March 2024

Background

The aim of this study was to analyze the concentration of leptin in peritoneal fluid and plasma and to assess their role as potential biomarkers in the diagnosis of endometriosis.

Materials & methods

Leptin adjusted for BMI (leptin/BMI ratio) was measured using surface plasmon resonance imaging (SPRI) biosensors. Patients with suspected endometriosis were included in the study. Plasma was collected from 70 cases, and peritoneal fluid from 67 cases. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group and a control group (patients without endometriosis).

Results

Leptin/BMI ratio in plasma did not differ between women with endometriosis and the control group (0.7159 ± 0.259 vs 0.6992 ± 0.273, p= 0,7988). No significant differences were observed in peritoneal leptin/BMI ratio levels in patients with and without endometriosis (0.6206 ± 0.258 vs 0.6215 ± 0.264, p= 0,9896). Plasma and peritoneal leptin/BMI ratios were significantly lower in women with endometriosis - related primary infertility compared to women with endometriosis without primary infertility (0.640 ± 0.502 vs 0.878 ± 0.623, p < 0.05). The difference was observed in case of primary infertility, but not in terms of the secondary one. No significant differences were noted between leptin/BMI ratio in the proliferative phase and the secretory phase (0.716 ± 0.252 vs 0.697 ± 0.288, p= 0,7785).

Conclusion

The results of present study do not support the relevance of leptin concentration determination as a biomarker of the endometriosis. Due to the limited number of samples in the tested group, further studies are needed to confirm its role.
The design of an improved index system for frequency control in China Southern Power Grid
Authors: Kuo Xin , Hongxuan Zhang , Wenzu Wu , Jingpeng Chen , Qixing Liu
Published: March 2024

Introduction: In order to dispatch frequency regulation resources in regional power grids efficiently and promote the development of spot markets, China Southern Power Grid (CSG) established the unified frequency regulation control area. However, the existing regional control performance standards (CPS) for evaluating the performance of frequency control in bulk power systems is no longer suitable for the unified frequency control mode.

Method: This paper proposed an innovative frequency control performance standard, named tertiary control performance standards (TCPS) and used Gaussian mixture model (GMM) and folded normal distribution (FND) to describe the distributions of frequency deviations and power deviations of tie line.

Result and Discussion: Based on these probability models, the parameters of the proposed TCPS can be determined optimized. Finally, case studies were carried out with the practical data from CSG and indicated that the parameters of proposed TCPS index could be calculated and improved the control performance for the real time power balance of the regional power grid with spot markets.
Efficacy of the induced pluripotent stem cell derived and engineered CD276-targeted CAR-NK cells against human esophageal squamous cell carcinoma
Authors: Lin Xiaolan , Guan Tian , Xu Yien , Li Yun , Lin Yanchun , Chen Shaobin , Chen Yuping , Wei Xiaolong , Li Dongsheng , Cui Yukun , Lin Yan , Sun Pingnan , Guo Jianmin , Li Congzhu , Gu Jiang , Yang Wei , Zeng Haoyu , Ma Changchun
Published: March 2024

Introduction

Chimeric antigen receptor natural killer (CAR-NK) cells have been found to be successful in treating hematologic malignancies and present potential for usage in solid tumors.

Methods

In this study, we created CD276-targeted CAR-expressing NK cells from pluripotent stem cells (iPSC CD276-targeted CAR-NK cells) and evaluated their cytotoxicity against esophageal squamous cell carcinoma (ESCC) using patient-specific organoid (PSO) models comprising of both CD276-positive and CD276-negative adjacent epithelium PSO models (normal control PSO, NC PSO) as well as primary culture of ESCC cell models. In addition, in vitro and in vivo models such as KYSE-150 were also examined. iPSC NK cells and NK-free media were used as the CAR-free and NK-free controls, respectively.

Results

The positive CD276 staining was specifically detected on the ESCC membrane in 51.43% (54/105) of the patients of all stages, and in 51.35% (38/74) of stages III and IV. The iPS CD276-targeted CAR-NK cells, comparing with the iPS NK cells and the NK-free medium, exhibited specific and significant cytotoxic activity against CD276-positive ESCC PSO rather than CD276-negative NC PSO, and exhibited significant cytotoxicity against CD276-expressing cultured ESCC cells, as well as against CD276-expressing KYSE-150 in vitro and in BNDG mouse xenograft.

Discussion

The efficacy of the iPSC CD276-targeted CAR-NK cells demonstrated by their successful treatment of CD276-expressing ESCC in a multitude of pre-clinical models implied that they hold tremendous therapeutic potential for treating patients with CD276-expressing ESCC.
Transcriptomics in idiopathic pulmonary fibrosis unveiled: a new perspective from differentially expressed genes to therapeutic targets
Authors: Hu Wenzhong , Xu Yun
Published: March 2024

Background

The underlying molecular pathways of idiopathic pulmonary fibrosis (IPF), a progressive lung condition with a high death rate, are still mostly unknown. By using microarray datasets, this study aims to identify new genetic targets for IPF and provide light on the genetic factors that contribute to the development of IPF.

Method

We conducted a comprehensive analysis of three independent IPF datasets from the Gene Expression Omnibus (GEO) database, employing R software for data handling and normalization. Our evaluation of the relationships between differentially expressed genes (DEGs) and IPF included differential expression analysis, expression quantitative trait loci (eQTL) analysis, and Mendelian Randomization(MR) analyses. Additionally, we used Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to explore the functional roles and pathways of these genes. Finally, we validated the results obtained for the target genes.

Results

We identified 486 highly expressed genes and 468 lowly expressed genes that play important roles in IPF. MR analysis identified six significantly co-expressed genes associated with IPF, specifically C12orf75, SPP1, ZG16B, LIN7A, PPP1R14A, and TLR2. These genes participate in essential biological processes and pathways, including macrophage activation and neural system regulation. Additionally, CIBERSORT analysis indicated a unique immune cell distribution in IPF, emphasized the significance of immunological processes in the disease. The MR analysis was consistent with the results of the analysis of variance in the validation cohort, which strengthens the reliability of our MR findings.

Conclusion

Our findings provide new insights into the molecular basis of IPF and highlight the promise of therapeutic interventions. They emphasize the potential of targeting specific molecular pathways for the treatment of IPF, laying the foundation for further research and clinical work.
Activated B-Cells enhance epitope spreading to support successful cancer immunotherapy
Authors: Kellermann Guillaume , Leulliot Nicolas , Cherfils-Vicini Julien , Blaud Magali , Brest Patrick
Published: March 2024

Immune checkpoint therapies (ICT) have transformed the treatment of cancer over the past decade. However, many patients do not respond or suffer relapses. Successful immunotherapy requires epitope spreading, but the slow or inefficient induction of functional antitumoral immunity delays the benefit to patients or causes resistances. Therefore, understanding the key mechanisms that support epitope spreading is essential to improve immunotherapy. In this review, we highlight the major role played by B-cells in breaking immune tolerance by epitope spreading. Activated B-cells are key Antigen-Presenting Cells (APC) that diversify the T-cell response against self-antigens, such as ribonucleoproteins, in autoimmunity but also during successful cancer immunotherapy. This has important implications for the design of future cancer vaccines.
A novel associative memory model based on semi-tensor product (STP)
Authors: Hou Yanfang , Tian Hui , Wang Chengmao
Published: March 2024

A good intelligent learning model is the key to complete recognition of scene information and accurate recognition of specific targets in intelligent unmanned system. This study proposes a new associative memory model based on the semi-tensor product (STP) of matrices, to address the problems of information storage capacity and association. First, some preliminaries are introduced to facilitate modeling, and the problem of information storage capacity in the application of discrete Hopfield neural network (DHNN) to associative memory is pointed out. Second, learning modes are equivalently converted into their algebraic forms by using STP. A memory matrix is constructed to accurately remember these learning modes. Furthermore, an algorithm for updating the memory matrix is developed to improve the association ability of the model. And another algorithm is provided to show how our model learns and associates. Finally, some examples are given to demonstrate the effectiveness and advantages of our results. Compared with mainstream DHNNs, our model can remember learning modes more accurately with fewer nodes.