bluuebunny/crossref_metadata_2025 huggingface dataset:
Subsidence of Uncemented Short Stems in Reverse Shoulder Arthroplasty—A Multicenter Study
Authors: Tross Anna-K. , Lädermann Alexandre , Wittmann Thomas , Schnetzke Marc , Nolte Philip-C. , Collin Philippe , Raiss Patric
Published: October 2020

Background: The radiological phenomenon of subsidence following the implantation of uncemented short-stem reverse prostheses (USSP) has not yet been described. The purpose of this study was to describe the rate and potential risk factors for subsidence. We hypothesized that subsidence may be a frequent finding and that a subsidence of >5 mm (mm) is associated with an inferior clinical outcome. Methods: A total of 139 patients with an average age of 73 ± 9 years were included. The clinical and radiological outcome was evaluated at a minimum follow-up (FU) of 12 months. Results: No humeral component loosening was present at a mean FU of 18 (range, 12–51) months. Mean Constant Score (CS) and Subjective Shoulder Value (SSV) improved significantly from 34.3 ± 18.0 points and 37.0 ± 19.5% preoperatively to 72.2 ± 13.4 points and 80.3 ± 16.5% at final FU (p < 0.001). The average subsidence of the USSP was 1.4 ± 3.7 mm. Subsidence of >5 mm was present in 15 patients (11%). No association between a subsidence >5 mm and CS or SSV was found (p = 0.456, p = 0.527). However, a subsidence of >5 mm resulted in lower strength at final FU (p = 0.022). Complications occurred in six cases (4.2%), and the revision rate was 3.5% (five cases). Conclusions: Although subsidence of USSP is a frequent radiographic finding it is not associated with loosening of the component or a decrease in the clinical outcome at short term FU. Level of evidence: Level 4, retrospective study.
Evaluation of Strip Meniscometry and Association with Clinical and Demographic Variables in a Community Eye Study (in Bangladesh)
Authors: Rashid Mamunur A.K.M. , Thia Zhang Zhe , Teo Calesta Hui Yi , Mamun Sumaiya , Ong Hon Shing , Tong Louis
Published: October 2020

Strip meniscometry (SM) is a relatively new technique for evaluating inferior tear meniscus. We described SM in an epidemiology study and its potential associations with clinical and tear parameters. This cross-sectional study involved 1050 factory garment workers in Gazipur, Bangladesh. The Ocular Surface Disease Index (OSDI) questionnaire and a standard examination for dry eye and meibomian gland dysfunction (MGD), including the five-second SM, were performed by a single ophthalmologist. The participants’ ages were 35.56 ± 12.12 years (range 18–59), with 53.8% women. The overall SM was 7.7 ± 3.6 mm, with skewness of 0.126 and kurtosis of 1.84 in frequency distribution. SM values were significantly lower in men than women, and significantly correlated with schirmers (r = 0.71) and tear break up time (TBUT) (r = 0.89). A lower SM value was associated with higher OSDI, lower Schirmer test, increased MG severity and lower TBUT. In multivariable analysis, when adjusted by age, SM values remained associated with schirmers and TBUT, and inversely associated with OSDI. In a separate regression model, higher SM was associated with increasing age, reduced severity of MGD grading, and increased TBUT. To conclude, SM is a rapid clinical test associated with dry eye symptoms and signs, with findings affected by both tear secretion and tear stability.
Changes in Metabolic Profile in the Women with a History of PCOS—A Long-Term Follow-Up Study
Authors: Jacewicz-Święcka Małgorzata , Kowalska Irina
Published: October 2020

Data concerning metabolic consequences in women with polycystic ovary syndrome (PCOS) are delivered mainly by cross-sectional studies. In this research, we re-examined 31 Caucasian PCOS women after a median period of 120.9 months to evaluate the changes in metabolic syndrome components. Clinical examination, oral glucose tolerance test with estimations of glucose and insulin, lipids, sex hormone-binding globulin (SHBG) and sex hormones assessments were performed on two occasions. Additionally, the euglycaemic hyperinsulinaemic clamp technique was used at the baseline to assess insulin sensitivity (M-clamp value). In the end, the median age of participants was 35. We observed an increase in glucose concentrations, a decrease in insulin concentrations and no changes in insulin resistance markers. Final mean glucose, mean insulin, Matsuda index and body mass index (BMI) were correlated with baseline M-clamp value and SHBG (p < 0.01). During the follow-up, no one in the sample developed diabetes. The annualised incidence rate for conversion from normoglycaemia to prediabetes totalled 4.5%. Baseline BMI, free androgen index, fasting glucose and M-clamp value were identified as prediabetes predictors in young PCOS women (respectively, OR = 1.17, OR = 1.42, OR = 1.2, OR = 0.73, p < 0.05). Prediabetes appeared in 76.47% of the women with a final BMI of ≥ 25 kg/m2 and in 7.14% of the normal-weight women (p = 0.0001). In conclusion, we report a high rate of adverse change in glucose metabolism in overweight and obese participants, a deterioration in β-cell function and strong correlations between metabolic parameters assessed in the third and the fourth decade in PCOS women, emphasising the role of early intervention to prevent cardiometabolic diseases.
Tropisms of the Dowser Texture
Authors: Pieranski Pawel , Godinho Maria Helena
Published: October 2020

Due to its low symmetry C2v, the dowser texture is characterised by a 2D unitary vector field or alternatively by a unitary complex field. For the same symmetry reasons, the dowser texture is sensitive, in first order, to perturbations such as thickness gradients, electric fields or flows. We will focus on corresponding properties called respectively: cuneitropism, electrotropism and rheotropism. In particular we will show that topological defects, known as dowsons or monopoles, can be manipulated by means of these tropisms.
Influence of Vinyl Polysiloxane Impression Techniques on Marginal Fit of Metal Frameworks for Fixed Partial Dentures
Authors: Nissan Joseph , Rosner Ofir , Rosen Gal , Naishlos Sarit , Zenziper Eran , Zelikman Helena , Lavi David , Chaushu Liat
Published: October 2020

Impression technique is one of the factors affecting restoration fit accuracy, which is a major aspect influencing its survival. The purpose of this study is to compare, in vivo, the effect of two commonly used Vinyl Polysiloxane (VPS) impression techniques on the metal framework fitting of fixed partial dentures. Ninety-two consecutive patients, diagnosed as partially edentulous, treated by fixed partial denture restorations, participated in the study. Group 1-impressions (n = 44) were subjected to the 1-step technique, while group 2 impressions (n = 48) were subjected the 2-step technique. Three accuracy assessment common methods: probe, tactile sense and radiographic test, were used to validate the clinical fit of the metal framework. Misfit was defined as even one test failure. Twenty-one (22.8%) out of 92 metal frameworks exhibited metal frameworks misfit, whereas the other 71 (77.2%) were found to be accurate. Group 1 presented significantly (p = 0.04) more metal frameworks misfit, 14/44 (31.8%) vs. 7/48 (14.6%). Restoration location (maxilla vs. mandible) had no statistically significant impact on the results (p = 0.461). The use of the VPS putty/wash 2-step impression technique is recommended to improve the clinical fit of fixed partial denture restorations.
Mitigating Portland Cement CO2 Emissions Using Alkali-Activated Materials: System Dynamics Model
Authors: Nehdi Moncef L. , Yassine Abdallah
Published: October 2020

While alkali-activated materials (AAMs) have been hailed as a very promising solution to mitigate colossal CO2 emissions from world portland cement production, there is lack of robust models that can demonstrate this claim. This paper pioneers a novel system dynamics model that captures the system complexity of this problem and addresses it in a holistic manner. This paper reports on this object-oriented modeling paradigm to develop a cogent prognostic model for predicting CO2 emissions from cement production. The model accounts for the type of AAM precursor and activator, the service life of concrete structures, carbonation of concrete, AAM market share, and policy implementation period. Using the new model developed in this study, strategies for reducing CO2 emissions from cement production have been identified, and future challenges facing wider AAM implementation have been outlined. The novelty of the model consists in its ability to consider the CO2 emission problem as a system of systems, treating it in a holistic manner, and allowing the user to test diverse policy scenarios, with inherent flexibility and modular architecture. The practical relevance of the model is that it facilitates the decision-making process and policy making regarding the use of AAMs to mitigate CO2 emissions from cement production at low computational cost.
Data Merging of AE Sensors with Different Frequency Resolution for the Detection and Identification of Damage in Oxide-Based Ceramic Matrix Composites
Authors: Guel Nicolas , Hamam Zeina , Godin Nathalie , Reynaud Pascal , Caty Olivier , Bouillon Florent , Paillassa Aude
Published: October 2020

In this paper, acoustic emission data fusion based on multiple measurements is presented for damage detection and identification in oxide-based ceramic matrix composites. Multi-AE (acoustic emission) sensor fusion is considered with the aim of a better identification of damage mechanisms. In this context, tensile tests were conducted on ceramic matrix composites, fabricated with 3M™ Nextel™ 610 fibers and aluminosilicate matrix, with two kinds of AE sensors. Redundant and complementary sensor data were merged to enhance AE system capability and reliability. Data fusion led to consistent signal clustering with an unsupervised procedure. A correlation between these clusters and the damage mechanisms was established thanks to in situ observations. The complementarity of the information from both sensors greatly improves the characterization of sources for their classification. Moreover, this complementarity allows features to be perceived more precisely than using only the information from one kind of sensor.
Analysis and Synthesis of Control Systems for Spacecraft Solar Arrays
Authors: Tishchenko Anatoly K. , Vasiljev Eugeny M. , Tishchenko Artyom O.
Published: October 2020

The paper is devoted to the problem of creating highly reliable power supply systems for spacecrafts intended for long-term autonomous flights. Within its framework, the problem of synthesizing a control system for solar arrays is being solved. To solve this problem, a mathematical model of a solar panel was compiled, and a study of its static and dynamic characteristics was carried out. It was found that when the solar panel is controlled using a shunt switch with pulse-width modulation, resonance phenomena appear in the system, leading to an unacceptable change in the polarity of voltages on the photocells. The operating conditions of the solar panels, which exclude the occurrence of the indicated alternating voltages, are found, and appropriate recommendations are given for the choice of the quantization frequency in the system. On the basis of the recommendations received, the transition to a quasi-continuous representation of the control system was carried out, and a graphic-analytical synthesis of the controller providing the required quality indicators of the system was carried out. To ensure the survivability of the power supply system, a method is proposed for the hierarchical organization of the interaction of solar panels, which reproduces the homeostatic properties of biological structures in the system. This property is provided by automatic transfer of control to subsequent levels of the hierarchy as the energy resources of the previous levels are exhausted. In addition, selective control is applied only to that part of the total generated power, which is sufficient to counter the current disturbing influences on the system. This approach to control prevents cascading failures in the system. The paper presents simulation models on which all theoretical positions and methods proposed in the work are tested.
Hydrodynamical Study of Creeping Maxwell Fluid Flow through a Porous Slit with Uniform Reabsorption and Wall Slip
Authors: Ullah Hameed , Lu Dianchen , Siddiqui Abdul Majeed , Haroon Tahira , Maqbool Khadija
Published: October 2020

The present theoretical study investigates the influence of velocity slip characteristics on the plane steady two-dimensional incompressible creeping Maxwell fluid flow passing through a porous slit with uniform reabsorption. This two-dimensional flow phenomenon is governed by the mathematical model having nonlinear partial differential equations together with non-homogeneous boundary conditions. An analytical technique, namely the recursive approach, is used successfully to find the solutions of the problem. The explicit expressions for stream function, velocity components, pressure distribution, wall shear stress and normal stress difference have been derived. The axial flow rate, leakage flux and fractional reabsorption are also found out. The points of maximum velocity are identified. Non-dimensionalization is carried out and graphs are portrayed at different positions of the channel to show the impact of pertinent parameters: slip parameter, Maxwell fluid parameter and absorption parameter, on flow variables and found that the fluid velocity is affected significantly due to these parameters. This study provides a mathematical basis to understand the physical phenomenon for fluid flows through permeable boundaries which exists in different problems like gaseous diffusion, filtration and biological mechanisms.
Compiled Constructions towards Post-Quantum Group Key Exchange: A Design from Kyber
Authors: Escribano Pablos José Ignacio , González Vasco María Isabel , Marriaga Misael Enrique , Pérez del Pozo Ángel Luis
Published: October 2020

A group authenticated key exchange (GAKE) protocol allows a set of parties belonging to a certain designated group to agree upon a common secret key through an insecure communication network. In the last few years, many new cryptographic tools have been specifically designed to thwart attacks from adversaries which may have access to (different kinds of) quantum computation resources. However, few constructions for group key exchange have been put forward. Here, we propose a four-round GAKE which can be proven secure under widely accepted assumptions in the Quantum Random Oracle Model. Specifically, we integrate several primitives from the so-called Kyber suite of post-quantum tools in a (slightly modified) compiler from Abdalla et al. (TCC 2007). More precisely, taking as a starting point an IND-CPA encryption scheme from the Kyber portfolio, we derive, using results from Hövelmanns et al. (PKC 2020), a two-party key exchange protocol and an IND-CCA encryption scheme and prove them fit as building blocks for our compiled construction. The resulting GAKE protocol is secure under the Module-LWE assumption, and furthermore achieves authentication without the use of (expensive) post-quantum signatures.
Nanoporous High-Entropy Alloy by Liquid Metal Dealloying
Authors: Okulov Artem Vladimirovich , Joo Soo-Hyun , Kim Hyoung Seop , Kato Hidemi , Okulov Ilya Vladimirovich
Published: October 2020

High-entropy nanomaterials possessing high accessible surface areas have demonstrated outstanding catalytic performance, beating that found for noble metals. In this communication, we report about the synthesis of a new, nanoporous, high-entropy alloy (HEA) possessing open porosity. The nanoporous, high-entropy Ta19.1Mo20.5Nb22.9V30Ni7.5 alloy (at%) was fabricated from a precursor (TaMoNbV)25Ni75 alloy (at%) by liquid metal dealloying using liquid magnesium (Mg). Directly after dealloying, the bicontinuous nanocomposite consisting of a Mg-rich phase and a phase with a bulk-centered cubic (bcc) structure was formed. The Mg-rich phase was removed with a 3M aqueous solution of nitric acid to obtain the open, porous, high-entropy Ta19.1Mo20.5Nb22.9V30Ni7.5 alloy (at%). The ligament size of this nanoporous HEA is about 69 ± 9 nm, indicating the high surface area in this material.
Morphology and Optical Properties of Thin Cd3As2 Films of a Dirac Semimetal Compound
Authors: Kovaleva Natalia , Fekete Ladislav , Chvostova Dagmar , Muratov Andrei
Published: October 2020

Using atomic-force microscopy (AFM) and wide-band (0.02–8.5 eV) spectroscopic ellipsometry techniques, we investigated the morphology and optical properties of Cd3As2 films grown by non-reactive RF magnetron sputtering on two types of oriented crystalline substrates (100)p-Si and (001) α-Al2O3. The AFM study revealed the grainy morphology of the films due to island incorporation during the film growth. The complex dielectric function spectra of the annealed Cd3As2/Al2O3 films manifest pronounced interband optical transitions at 1.2 and 3.0 eV, in excellent agreement with the theoretical calculations for the body centered tetragonal Cd3As2 crystal structure. We discovered that due to electronic excitations to the Cd(s) conical bands, the low-energy absorption edge of the annealed Cd3As2 films reveals a linear dependence. We found that for the annealed Cd3As2 films, the Cd(s) conical node may be shifted in energy by about 0.08–0.18 eV above the heavy-flat As(p) valence band, determining the optical gap value. The as-grown Cd3As2 films exhibit the pronounced changes of the electronic band structure due to the doping effect associated with Cd non-stoichiometry, where fine-tuning of the Cd concentration may result in the gapless electronic band structure of Dirac semimetals.
Formation Mechanism of Micro- and Nanocrystalline Surface Layers in Titanium and Aluminum Alloys in Electron Beam Irradiation
Authors: Nevskii Sergei , Sarychev Vladimir , Konovalov Sergey , Granovskii Alexey , Gromov Victor
Published: October 2020

The reported study discusses the formation of micro- and nanocrystalline surface layers in alloys on the example of Ti-Y and Al-Si-Y systems irradiated by electron beams. The study has established a crystallization mechanism of molten layers in the micro-and nanodimensional range, which involves a variety of hydrodynamic instabilities developing on the plasma–melt interface. As suggested, micro- and nanostructures form due to the combination of thermocapillary, concentration and capillary, evaporation and capillary and thermoelectric instabilities. This mechanism has provided the foundation for a mathematical model to describe the development of structures in focus in the electron beam irradiation. The study has pointed out that thermoelectric field strength E ≥ 106 V/m is attributed to the occurring combination of instabilities in micro- and nanodimensional ranges. A full dispersion equation of perturbations on the melt surface was analyzed.
Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis
Authors: Dubrulle Guillaume , Picot Adeline , Madec Stéphanie , Corre Erwan , Pawtowski Audrey , Baroncelli Riccardo , Zivy Michel , Balliau Thierry , Le Floch Gaétan , Pensec Flora
Published: October 2020

The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.
Novel Nucleoside Analogues as Effective Antiviral Agents for Zika Virus Infections
Authors: Bassetto Marcella , Cima Cecilia M. , Basso Mattia , Salerno Martina , Schwarze Frank , Friese Daniela , Bugert Joachim J. , Brancale Andrea
Published: October 2020

Previously considered a neglected flavivirus, Zika virus has recently emerged as a public health concern due to its ability to spread rapidly and cause severe neurological disorders, such as microcephaly in newborn babies from infected mothers, and Guillain-Barré syndrome in adults. Despite extensive efforts towards the identification of effective therapies, specific antivirals are still not available. As part of ongoing medicinal chemistry studies to identify new antiviral agents, we screened against Zika virus replication in vitro in a targeted internal library of small-molecule agents, comprising both nucleoside and non-nucleoside agents. Among the compounds evaluated, novel aryloxyphosphoramidate prodrugs of the nucleosides 2′-C-methyl-adenosine, 2-CMA, and 7-deaza-2′C-methyl-adenosine, 7-DMA, were found to significantly inhibit the virus-induced cytopathic effect in multiple relevant cell lines. In addition, one of these prodrugs exhibits a synergistic antiviral effect against Zika virus when applied in combination with an indirect antiviral agent, a l-dideoxy bicyclic pyrimidine nucleoside analogue, which potently inhibits vaccinia and measles viruses in vitro by targeting a host pathway. Our findings provide a solid basis for further development of an antiviral therapy for Zika virus infections, possibly exploiting a dual approach combining two different agents, one targeting the viral polymerase (direct-acting antiviral), the second targeting a host-directed autophagy mechanism.
Seeding, Plating and Electrical Characterization of Gold Nanowires Formed on Self-Assembled DNA Nanotubes
Authors: Ranasinghe Dulashani R. , Aryal Basu R. , Westover Tyler R. , Jia Sisi , Davis Robert C. , Harb John N. , Schulman Rebecca , Woolley Adam T.
Published: October 2020

Self-assembly nanofabrication is increasingly appealing in complex nanostructures, as it requires fewer materials and has potential to reduce feature sizes. The use of DNA to control nanoscale and microscale features is promising but not fully developed. In this work, we study self-assembled DNA nanotubes to fabricate gold nanowires for use as interconnects in future nanoelectronic devices. We evaluate two approaches for seeding, gold and palladium, both using gold electroless plating to connect the seeds. These gold nanowires are characterized electrically utilizing electron beam induced deposition of tungsten and four-point probe techniques. Measured resistivity values for 15 successfully studied wires are between 9.3 × 10−6 and 1.2 × 10−3 Ωm. Our work yields new insights into reproducible formation and characterization of metal nanowires on DNA nanotubes, making them promising templates for future nanowires in complex electronic circuitry.
Chronological Review and Rational and Future Prospects of Cannabis-Based Drug Development
Authors: Namdar Dvora , Anis Omer , Poulin Patrick , Koltai Hinanit
Published: October 2020

Despite the surge in cannabis chemistry research and its biological and medical activity, only a few cannabis-based pharmaceutical-grade drugs have been developed and marketed to date. Not many of these drugs are Food and Drug Administration (FDA)-approved, and some are still going through regulation processes. Active compounds including cannabinergic compounds (i.e., molecules targeted to modulate the endocannabinoid system) or phytocannabinoid analogues (cannabinoids produced by the plant) may be developed into single-molecule drugs. However, since in many cases treatment with whole-plant extract (whether as a solvent extraction, galenic preparation, or crude oil) is preferred over treatment with a single purified molecule, some more recently developed cannabis-derived drugs contain several molecules. Different combinations of active plant ingredients (API) from cannabis with proven synergies may be identified and developed as drugs to treat different medical conditions. However, possible negative effects between cannabis compounds should also be considered, as well as the effect of the cannabis treatment on the endocannabinoid system. FDA registration of single, few, or multiple molecules as drugs is a challenging process, and certain considerations that should be reviewed in this process, including issues of drug–drug interactions, are also discussed here.
Targeting Metabolism in Cancer Cells and the Tumour Microenvironment for Cancer Therapy
Authors: Li Jiaqi , Eu Jie Qing , Kong Li Ren , Wang Lingzhi , Lim Yaw Chyn , Goh Boon Cher , Wong Andrea L. A.
Published: October 2020

Targeting altered tumour metabolism is an emerging therapeutic strategy for cancer treatment. The metabolic reprogramming that accompanies the development of malignancy creates targetable differences between cancer cells and normal cells, which may be exploited for therapy. There is also emerging evidence regarding the role of stromal components, creating an intricate metabolic network consisting of cancer cells, cancer-associated fibroblasts, endothelial cells, immune cells, and cancer stem cells. This metabolic rewiring and crosstalk with the tumour microenvironment play a key role in cell proliferation, metastasis, and the development of treatment resistance. In this review, we will discuss therapeutic opportunities, which arise from dysregulated metabolism and metabolic crosstalk, highlighting strategies that may aid in the precision targeting of altered tumour metabolism with a focus on combinatorial therapeutic strategies.
Evaluation of Cytotoxicity and α-Glucosidase Inhibitory Activity of Amide and Polyamino-Derivatives of Lupane Triterpenoids
Authors: Kazakova Oxana B. , Giniyatullina Gul’nara V. , Mustafin Akhat G. , Babkov Denis A. , Sokolova Elena V. , Spasov Alexander A.
Published: October 2020

A series of two new and twenty earlier synthesized branched extra-amino-triterpenoids obtained by the direct coupling of betulinic/betulonic acids with polymethylenpolyamines, or by the cyanoethylation of lupane type alcohols, oximes, amines, and amides with the following reduction were evaluated for cytotoxicity toward the NCI-60 cancer cell line panel, α-glucosidase inhibitory, and antimicrobial activities. Lupane carboxamides, conjugates with diaminopropane, triethylenetetramine, and branched C3-cyanoethylated polyamine methyl betulonate showed high cytotoxic activity against most of the tested cancer cell lines with GI50 that ranged from 1.09 to 54.40 µM. Betulonic acid C28-conjugate with triethylenetetramine and C3,C28-bis-aminopropoxy-betulin were found to be potent micromolar inhibitors of yeast α-glucosidase and to simultaneously inhibit the endosomal reticulum α-glucosidase, rendering them as potentially capable to suppress tumor invasiveness and neovascularization, in addition to the direct cytotoxicity. Plausible mechanisms of cytotoxic action and underlying disrupted molecular pathways were elucidated with CellMinner pattern analysis and Gene Ontology enrichment analysis, according to which the lead compounds exert multi-target antiproliferative activity associated with oxidative stress induction and chromatin structure alteration. The betulonic acid diethylentriamine conjugate showed partial activity against methicillin-resistant S. aureus and the fungi C. neoformans. These results show that triterpenic polyamines, being analogs of steroidal squalamine and trodusquemine, are important substances for the search of new drugs with anticancer, antidiabetic, and antimicrobial activities.
Voltage-Controlled Anodic Oxidation of Porous Fluorescent SiC for Effective Surface Passivation
Authors: Yanai Kosuke , Lu Weifang , Yamane Yoma , Han Dong-Pyo , Ou Haiyan , Iwaya Motoaki , Takeuchi Tetsuya , Kamiyama Satoshi , Akasaki Isamu
Published: October 2020

This study investigated the fabrication of porous fluorescent SiC using a constant voltage-controlled anodic oxidation process. The application of a high, constant voltage resulted in a spatial distinction between the porous structures formed inside the fluorescent SiC substrates, due to the different etching rates at the terrace and the large step bunches. Large, dendritic porous structures were formed as the etching process continued and the porous layer thickened. Under the conditions of low hydrofluoric acid (HF) concentration, the uniformity of the dendritic porous structures through the entire porous layer was considerably improved compared with the conditions of high HF concentration. The resulting large uniform structure offered a sizable surface area, and promoted the penetration of atomic layer-deposited (ALD) Al2O3 films (ALD–Al2O3). The emission intensity in the porous fluorescent SiC was confirmed via photoluminescence (PL) measurements to be significantly improved by a factor of 128 after ALD passivation. With surface passivation, there was a clear blueshift in the emission wavelength, owing to the effective suppression of the non-radiative recombination rate in the porous structures. Furthermore, the spatial uniformity of emitted light was examined via PL mapping using three different excitation lasers, which resulted in the observation of uniform and distinctive emissions in the fluorescent SiC bulk and porous areas.
Non-Absorbing Dielectric Materials for Surface-Enhanced Spectroscopies and Chiral Sensing in the UV
Authors: Rosales Saúl A. , González Francisco , Moreno Fernando , Gutiérrez Yael
Published: October 2020

Low-loss dielectric nanomaterials are being extensively studied as novel platforms for enhanced light-matter interactions. Dielectric materials are more versatile than metals when nanostructured as they are able to generate simultaneously electric- and magnetic-type resonances. This unique property gives rise to a wide gamut of new phenomena not observed in metal nanostructures such as directional scattering conditions or enhanced optical chirality density. Traditionally studied dielectrics such as Si, Ge or GaP have an operating range constrained to the infrared and/or the visible range. Tuning their resonances up to the UV, where many biological samples of interest exhibit their absorption bands, is not possible due to their increased optical losses via heat generation. Herein, we report a quantitative survey on the UV optical performance of 20 different dielectric nanostructured materials for UV surface light-matter interaction based applications. The near-field intensity and optical chirality density averaged over the surface of the nanoparticles together with the heat generation are studied as figures of merit for this comparative analysis.
Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor
Authors: Oseev Aleksandr , Mukhin Nikolay , Elie-Caille Céline , Boireau Wilfrid , Lucklum Ralf , Lecompte Thomas , Remy-Martin Fabien , Manceau Jean-François , Chollet Franck , Leblois Thérèse
Published: October 2020

Shear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells while keeping the same basic assumptions. Thus, the biosensor readouts are still quite often described using ‘mass’ related terminology. This contribution aims to show that assessment of cell deposits with acoustic biosensors requires a deep understanding of the sensor transduction mechanism. More specifically, the cell deposits should be considered as a structured viscoelastic load and the sensor response depends on both material and topological parameters of the deposits. This shifts the paradigm of acoustic biosensor away from the classical mass loading perspective. As a proof of the concept, we recorded QCM frequency shifts caused by blood platelet deposits on a collagen surface under different rheological conditions and observed the final deposit shape with atomic force microscopy (AFM). The results vividly demonstrate that the frequency shift is highly impacted by the platelet topology on the bio-interface. We support our findings with numerical simulations of viscoelastic unstructured and structured loads in liquid. Both experimental and theoretical studies underline the complexity behind the frequency shift interpretation when acoustic biosensing is used with cell deposits.
Liposome-Mediated Inhibition of Inflammation by Hydroxycitrate
Authors: Vassallo Antonio , Santoro Valentina , Pappalardo Ilaria , Santarsiero Anna , Convertini Paolo , De Luca Maria , Martelli Giuseppe , Infantino Vittoria , Caddeo Carla
Published: October 2020

Hydroxycitrate (HCA), a main organic acid component of the fruit rind of Garcinia cambogia, is a natural citrate analog that can inhibit the ATP citrate lyase (ACLY) enzyme with a consequent reduction of inflammatory mediators (i.e., nitric oxide (NO), reactive oxygen species (ROS), and prostaglandin E2 (PGE2)) levels. Therefore, HCA has been proposed as a novel means to prevent, treat, and ameliorate conditions involving inflammation. However, HCA presents a low membrane permeability, and a large quantity is required to have a biological effect. To overcome this problem, HCA was formulated in liposomes in this work, and the enhancement of HCA cell availability along with the reduction in the amount required to downregulate NO, ROS, and PGE2 in macrophages were assessed. The liposomes were small in size (~60 nm), monodispersed, negatively charged (−50 mV), and stable on storage. The in vitro results showed that the liposomal encapsulation increased by approximately 4 times the intracellular accumulation of HCA in macrophages, and reduced by 10 times the amount of HCA required to abolish LPS-induced NO, ROS, and PGE2 increase. This suggests that liposomal HCA can be exploited to target the citrate pathway involved in inflammatory processes.
Synthesis, Electrocatalytic and Gas Transport Characteristics of Pentagonally Structured Star-Shaped Nanocrystallites of Pd-Ag
Authors: Petriev Iliya , Pushankina Polina , Lutsenko Ivan , Shostak Nikita , Baryshev Mikhail
Published: October 2020

The method of synthesis of bimetallic Pd–Ag pentagonally structured catalyst “nanostar” on the surface of Pd-23%Ag alloy films has been developed. The resulting catalyst was studied as a highly active functional layer for methanol oxidation reaction (MOR) in alkaline media and the intensification of hydrogen transport through the Pd-23%Ag membrane in the processes of hydrogen diffusion purification. A modifying layer with a controlled size, composition and excellent electrocatalytic activity was synthesized by electrochemical deposition at a reduced current density compared to classical methods. The low deposition rate affects the formation of pentagonally structured nanocrystallites, allowing Pd and Ag particles to form well-defined structures due to the properties of the surfactant used. Electrochemical studies have demonstrated that the catalyst synthesized by the “nanostar” method shows better electrocatalytic activity in relation to MOR and demonstrates a higher peak current (up to 17.82 µA cm−2) in comparison with one for the catalyst synthesized by the “nanoparticle” method (up to 10.66 µA cm−2) in a cyclic voltammetric study. The nanostar catalyst electrode releases the highest current density (0.25 µA cm−2) for MOR and demonstrates higher catalytic activity for the oxidation of possible intermediates such as sodium formate in MOR. In the processes of diffusion membrane purification of hydrogen, a multiple increase in the density of the penetrating flux of hydrogen through the membranes modified by the “nanostar” catalyst (up to 10.6 mmol s−1 m−2) was demonstrated in comparison with the membranes modified by the “nanoparticles” method (up to 4.49 mmol s−1 m−2). Research data may indicate that the properties of the developed pentagonally structured catalyst “nanostar” and its enhanced activity with respect to reactions involving hydrogen increase the desorption activity of the membrane, which ultimately accelerates the overall stepwise transfer of hydrogen across the membrane.
Ceruloplasmin and Coronary Heart Disease—A Systematic Review
Authors: Arenas de Larriva Antonio P. , Limia-Pérez Laura , Alcalá-Díaz Juan F. , Alonso Alvaro , López-Miranda José , Delgado-Lista Javier
Published: October 2020

Several studies indicate that oxidative stress might play a central role in the initiation and maintenance of cardiovascular diseases. It remains unclear whether ceruloplasmin acts as a passive marker of inflammation or as a causal mediator. To better understand the impact of ceruloplasmin blood levels on the risk of cardiovascular disease, and paying special attention to coronary heart disease, we conducted a search on the two most commonly used electronic databases (Medline via PubMed and EMBASE) to analyze current assessment using observational studies in the general adult population. Each study was quality rated using criteria developed by the US Preventive Services Task Force. Most of 18 eligible studies reviewed support a direct relationship between ceruloplasmin elevated levels and incidence of coronary heart disease. Our results highlight the importance of promoting clinical trials that determine the functions of ceruloplasmin as a mediator in the development of coronary heart disease and evaluate whether the treatment of elevated ceruloplasmin levels has a role in the prognosis or prevention of this condition.
Hepatitis E Virus Infection: Circulation, Molecular Epidemiology, and Impact on Global Health
Authors: Pallerla Srinivas Reddy , Harms Dominik , Johne Reimar , Todt Daniel , Steinmann Eike , Schemmerer Mathias , Wenzel Jürgen J. , Hofmann Jörg , Shih James Wai Kuo , Wedemeyer Heiner , Bock C.-Thomas , Velavan Thirumalaisamy P.
Published: October 2020

Infection with hepatitis E virus (HEV) represents the most common source of viral hepatitis globally. Although infecting over 20 million people annually in endemic regions, with major outbreaks described since the 1950s, hepatitis E remains an underestimated disease. This review gives a current view of the global circulation and epidemiology of this emerging virus. The history of HEV, from the first reported enteric non-A non-B hepatitis outbreaks, to the discovery of the viral agent and the molecular characterization of the different human pathogenic genotypes, is discussed. Furthermore, the current state of research regarding the virology of HEV is critically assessed, and the challenges towards prevention and diagnosis, as well as clinical risks of the disease described. Together, these points aim to underline the significant impact of hepatitis E on global health and the need for further in-depth research to better understand the pathophysiology and its role in the complex disease manifestations of HEV infection.
Control of Cytokines in Latent Cytomegalovirus Infection
Authors: Chinta Pearley , Garcia Erica C. , Tajuddin Kiran Hina , Akhidenor Naomi , Davis Allyson , Faure Lionel , Spencer Juliet V.
Published: October 2020

Human cytomegalovirus (HCMV) has evolved a number of mechanisms for long-term co-existence within its host. HCMV infects a wide range of cell types, including fibroblasts, epithelial cells, monocytes, macrophages, dendritic cells, and myeloid progenitor cells. Lytic infection, with the production of infectious progeny virions, occurs in differentiated cell types, while undifferentiated myeloid precursor cells are the primary site of latent infection. The outcome of HCMV infection depends partly on the cell type and differentiation state but is also influenced by the composition of the immune environment. In this review, we discuss the role of early interactions between HCMV and the host immune system, particularly cytokine and chemokine networks, that facilitate the establishment of lifelong latent infection. A better understanding of these cytokine signaling pathways could lead to novel therapeutic targets that might prevent latency or eradicate latently infected cells.
A Non-Enzymatic Sensor Based on Fc-CHIT/CNT@Cu Nanohybrids for Electrochemical Detection of Glucose
Authors: Wang Fang , Hu Sheng , Shi Fengna , Huang Kexin , Li Jiarui
Published: October 2020

Herein, a composite structure, consisting of Cu nanoparticles (NPs) deposited onto carbon nanotubes and modified with ferrocene-branched chitosan, was prepared in order to develop a nonenzymatic electrochemical glucose biosensor ferrocene-chitosan/carbon nanotube@ Cu (Fc-CHIT/CNT@Cu). The elemental composition of the carbon nanohybrids, morphology and structure were characterized by various techniques. Electrochemical impedance spectroscopy (EIS) was used to study the interfacial properties of the electrodes. Cyclic voltammetry (CV) and chronoamperometry methods in alkaline solution were used to determine glucose biosensing properties. The synergy effect of Cu NPs and Fc on current responses of the developed electrode resulted in good glucose sensitivity, including broad linear detection between 0.2 mM and 22 mM, a low detection limit of 13.52 μM and sensitivity of 1.256 μA mM−1cm−2. Moreover, the modified electrode possessed long-term stability and good selectivity in the presence of ascorbic acid, dopamine and uric acid. The results indicated that this inexpensive electrode had potential application for non-enzymatic electrochemical glucose detection.
Stress-Induced Alteration in Chloride Transporters in the Trigeminal Nerve May Explain the Comorbidity between Depression and Migraine
Authors: Kalkman Hans O.
Published: October 2020

Migraine is frequently comorbid with depression and anxiety disorders. In the case of depression and panic disorder, the associations seem to be bidirectional. Stress (activation of the hypothalamic-pituitary-adrenal axis) is thought to be involved in increasing the attack frequency. In the current review, it is argued that elevated levels of cortisol increase the function of chloride-ion transporter NKCC1 and decrease the function of chloride-extruder KCC2 in the trigeminal nerve. This leads to a diminished inhibitory effect of gamma-aminobutyric acid (GABA) and an enhanced likelihood of a migraine attack. Since migraine attacks themselves are stressful, and since brain areas are activated that could contribute to panic, anxiety and depression, a number of self-sustaining circular processes could occur that would explain the bi-directionality of the associations. On the basis of this hypothesis, several novel therapeutic approaches to counter the pathological process can be proposed. These include inhibition of corticotrophin releasing factor by CRF1 receptor antagonists, blockade of adrenocorticotropic hormone (ACTH) at the MC2 receptor, and inhibition of the hyperactive NKCC1 chloride-transporter.
Be Gentle to Them: Animal Welfare and the Protection of Draft Animals in the Ottoman Fatwā Literature and Legislation
Authors: Kızılkaya Necmettin
Published: October 2020

Animal studies in the Islamic context have greatly increased in number in recent years. These studies mostly examine the subject of animal treatment through the two main sources of Islam, namely, the Qur’an and the traditions of the Prophet Muhammad. Some studies that go beyond this examine the subject of animal treatment through the texts of various disciplines, especially that of Islamic jurisprudence and law. Although these two research approaches draw a picture on the subject of animal treatment, it is still not a full one. Other sources, such as fatwā books and archive documents, should be used to fill in the gaps. By incorporating these into the pool of research, we will be better enabled to understand how the principles expressed in the main sources of Islam are reflected in daily life. In this article, I shall examine animal welfare and animal protection in the Ottoman context based on the fatāwā of Shaykh al-Islām Ebū’s-Suʿūd Efendi and archival documents.
Narratives in Action: Modelling the Types and Drivers of Sikh Activism in Diaspora
Authors: Singh Jasjit
Published: October 2020

Using data gathered for an investigation of “Sikh radicalisation in Britain”, in this article I develop a typology of different types of activism among Sikhs in diaspora based on an analysis of historic and contemporary media sources (newspapers, radio, television, online), academic literature, ethnographic fieldwork and a series of semi-structured interviews with self-identifying Sikh activists. I assess the reasons behind a variety of different incidents involving Sikh activists, how Sikh activists view the drivers of their activism and to what extent this activism can be regarded as being “religiously motivated”. I critique existing typologies of “religious activism” by developing a typology of Sikh activism which challenges the distinction often made between “religious” and “political” action. I argue that “religiously motivated actions” must be understood in conjunction with narratives, incidents and issues specific to particular religious traditions and that generic motivations for these actions cannot be applied across all religious traditions.
Static Ice Pressure Measuring System Based on Fiber Loop Ring-Down Spectroscopy and FPGA
Authors: Deng Xiao , Wang Dingrui , Pan Lipeng , Zhang Li , Zhang Jun , Lu Xinshuo , Du Chao , Zhang Lin
Published: October 2020

Hydraulic engineering built in the cold region, such as reservoirs and hydropower stations, is often threatened by static ice pressure from nature. Therefore, it is of vital significance to research the pressure variation in the growth and melting processes of the ice layer for the design and protection of hydraulic structures in cold regions. This paper introduces an optical fiber sensor system based on the fiber loop ring-down spectroscopy technology and field-programmable gate array (FPGA) pulse modulation technology. An electro-optic modulation scheme that relied on FPGA to generate optical pulses with adjustable pulse width and period is proposed, which is more suitable for the in-situ observation. In addition, the temperature stability and repeatability of the system are also discussed. This system was applied to the real-time detection of static ice pressure on the sidewall and bottom of the polyvinyl chloride (PVC) pipe during the ice growth and melting processes. The results indicate that the system has favorable stability and sensitivity, and the relationship obtained between the static ice pressure and temperature could provide some references for the field application in the future.