bluuebunny/crossref_metadata_2025 huggingface dataset:
A controlled aquarium system and approach to study the role of sponge-bacteria interactions using Aplysilla rosea and Vibrio natriegens
Authors: Mehbub Mohammad F. , Tanner Jason E. , Barnett Stephen J. , Bekker Jan , Franco Christopher M. M. , Zhang Wei
Published: August 2018

Sponge-bacteria interactions are very important due to their ecological and biological significance. To understand the impact of interactions between sponges and bacteria (both associated with and external to sponges) on sponge-associated microbial diversity, sponge metabolite profiles and bioactivity, we used a controlled aquarium system and designed an experimental approach that allows the study of sponge-bacteria interactions in a well-defined manner. To test the feasibility of this approach, this system was used to study the interaction between a spongeAplysilla roseaand a marine bacterium commonly found in seawater,Vibrio natriegens. Sponge explants were exposed toV.natriegens, at 5 × 106cfu/ml, and changes were monitored for 48 hours. Pyro-sequencing revealed significant shifts in microbial communities associated with the sponges after 24 to 48 hours. Both the control (sponge only without added bacteria) andVibrio-exposed sponges showed a distinct shift in bacterial diversity and abundance with time.Vibrioexposure significantly increased bacterial diversity, the abundance of a number of taxa compared to control sponges. The result experimentally supports the notion of dynamic and concerted responses by the sponge when interacting with a bacterium, and demonstrates the feasibility of using this controlled aquarium system for the study of sponge-bacteria interactions.
Comparative effect of immunotherapy and standard therapy in patients with high grade glioma: a meta-analysis of published clinical trials
Authors: Artene Stefan-Alexandru , Turcu-Stiolica Adina , Ciurea Marius Eugen , Folcuti Catalin , Tataranu Ligia Gabriela , Alexandru Oana , Purcaru Oana Stefana , Tache Daniela Elise , Boldeanu Mihail Virgil , Silosi Cristian , Dricu Anica
Published: August 2018

Immunotherapy holds great promise in the treatment of high grade glioma (HGG). We performed a comprehensive meta-analysis of clinical trials involving dendritic cell (DC) therapy and viral therapy (VT) for the treatment of HGG, in order to assess their clinical impact in comparison to standard treatments in terms of overall survival (OS) and progression-free survival (PFS). To our knowledge, this is the first meta-analysis to evaluate VT for the treatment of HGG, allowing comparison of different immunotherapeutic approaches. Thirteen eligible studies of 1043 cases were included in the meta-analysis. For DC vaccination, in terms of OS, both newly diagnosed patients (HR, 0.65) and patients who suffered from recurrent HGGs (HR = 0.63) presented markedly improved results compared to the control groups. PFS was also improved (HR = 0.49) but was not statistically significant (p = 0.1). A slight improvement was observed for newly diagnosed patients receiving VT in terms of OS (HR = 0.88) while PFS was inferior for patients in the experimental arm (HR = 1.16). Our results show that DC therapy greatly improves OS for patients with both newly diagnosed and recurrent HGGs. VT, however, did not provide any statistically significant improvements in terms of OS and PFS for patients with newly diagnosed HGGs.
ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
Authors: Singh Shashi Kant , Sung Tzu-Ying , Chung Tsui-Yun , Lin Shao-Yu , Lin Sang-Chu , Liao Jo-Chien , Hsieh Wei-Yu , Hsieh Ming-Hsiun
Published: August 2018

The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which do not contain any recognizable catalytic domain. Arabidopsis has 12 ACR proteins, whose functions are largely unknown. To study the functions of Arabidopsis ACR11, we have characterized two independent T-DNA insertion mutants, acr11-2 and acr11-3. RNA gel-blot analysis revealed that the expression of wild-type ACR11 transcripts was not detectable in the acr11 mutants. Interestingly, a lesion-mimic phenotype occurs in some rosette leaves of the acr11 mutants. In addition, high levels of reactive oxygen species (ROS), salicylic acid (SA), and callose accumulate in the mutant leaves when grown under normal conditions. The expression of several SA marker genes and the key SA biosynthetic gene ISOCHORISMATE SYNTHASE1 is up-regulated in the acr11 mutants. Furthermore, the acr11 mutants are more resistant to the infection of bacterial pathogen Pseudomonas syringae pathovar tomato DC3000. These results suggest that ACR11 may be directly or indirectly involved in the regulation of ROS and SA accumulation, which in turn modulates SA-associated defense responses and disease resistance in Arabidopsis.
Identifying individuals with attention deficit hyperactivity disorder based on temporal variability of dynamic functional connectivity
Authors: Wang Xun-Heng , Jiao Yun , Li Lihua
Published: August 2018

Attention deficit hyperactivity disorder (ADHD) is a common disorder that emerges in school-age children. The diagnostic model based on neuroimaging features could be beneficial for ADHD in twofold: identifying individuals with ADHD and discovering the discriminative patterns for patients. The dynamic functional connectivity of ADHD remains unclear. Towards this end, 100 children with ADHD and 140 normal controls were obtained from the ADHD-200 Consortium. The raw features were derived from the temporal variability between intrinsic connectivity networks (ICNs) as well as the demographic and covariate variables. The diagnostic model was based on the support vector machines (SVMs). The performance of diagnostic model was analyzed using leave-one-out cross-validation (LOOCV) and 10-folds cross-validations (CVs). The diagnostic model based on inter-ICN variability outperformed that based on inter-ICN functional connectivity and inter-ICN phase synchrony. The LOOCV achieved total accuracy of 78.75%, the sensitivity of 76%, and the specificity of 80.71%. The 10-folds CVs achieved average prediction accuracy of 75.54% ± 1.34%, average sensitivity of 70.5% ± 2.34%, and average specificity of 77.44% ± 1.47%. In addition, the discriminative patterns for ADHD were discovered using SVMs. The discriminative patterns confirmed with previous findings. In summary, individuals with ADHD could be identified through inter-ICN variability, which could be potential biomarkers for diagnostic model of ADHD.
Design and MinION testing of a nanopore targeted gene sequencing panel for chronic lymphocytic leukemia
Authors: Orsini Paola , Minervini Crescenzio F. , Cumbo Cosimo , Anelli Luisa , Zagaria Antonella , Minervini Angela , Coccaro Nicoletta , Tota Giuseppina , Casieri Paola , Impera Luciana , Parciante Elisa , Brunetti Claudia , Giordano Annamaria , Specchia Giorgina , Albano Francesco
Published: August 2018

We report a customized gene panel assay based on multiplex long-PCR followed by third generation sequencing on nanopore technology (MinION), designed to analyze five frequently mutated genes in chronic lymphocytic leukemia (CLL): TP53, NOTCH1, BIRC3, SF3B1 and MYD88. For this purpose, 12 patients were selected according to specific cytogenetic and molecular features significantly associated with their mutational status. In addition, simultaneous analysis of the targets genes was performed by molecular assays or Sanger Sequencing. Data analysis included mapping to the GRCh37 human reference genome, variant calling and annotation, and average sequencing depth/error rate analysis. The sequencing depth resulted on average higher for smaller amplicons, and the final breadth of coverage of the panel was 94.1%. The error rate was about 6% and 2% for insertions/deletions and single nucleotide variants, respectively. Our gene panel allows analysis of the prognostically relevant genes in CLL, with two PCRs per patient. This strategy offers an easy and affordable workflow, although further advances are required to improve the accuracy of the technology and its use in the clinical field. Nevertheless, the rapid and constant development of nanopore technology, in terms of chemistry advances, more accurate basecallers and analysis software, offers promise for a wide use of MinION in the future.
Use of user-centered design to create a smartphone application for patient-reported outcomes in atopic dermatitis
Authors: Gracey Lia E. , Zan Shiyi , Gracz Joseph , Miner John J. , Moreau Jacqueline F. , Sperber Jodi , Jethwani Kamal , Hale Timothy M. , Kvedar Joseph C.
Published: August 2018

The ubiquity and convenience of smartphones carries great potential for collecting patient-reported data to address many gaps in research, especially those that rely on ongoing, real-time data collection. Health care apps have often suffered from low utility due to lack of consideration of the needs of multiple stakeholders. We employed an iterative user-centered design approach to create the myEczema smartphone application (app) to study the burden of disease of atopic dermatitis. We outline below the steps we took for developing myEczema for multiple stakeholders, including patients, clinicians, and researchers.
Smartphone measures of day-to-day behavior changes in children with autism
Authors: Jones Rebecca M. , Tarpey Thaddeus , Hamo Amarelle , Carberry Caroline , Lord Catherine
Published: August 2018

Smartphones offer a flexible tool to collect data about mental health, but less is known about their effectiveness as a method to assess variability in children’s problem behaviors. Caregivers of children with autism completed daily questions about irritability, anxiety and mood delivered via smartphones across 8-weeks. Smartphone questions were consistent with subscales on standard caregiver questionnaires. Data collection from 7 to 10 days at the beginning and 7 to 10 days at the end of the study were sufficient to capture similar amounts of variance as daily data across 8-weeks. Other significant findings included effects of caregiver socioeconomic status and placebo-like effects from participation even though the study included no specific treatment. Nevertheless, single questions via smartphones collected over relatively brief periods reliably represent subdomains in standardized behavioral questionnaires, thereby decreasing burden on caregivers.
Bioluminescence resonance energy transfer-based biosensors allow monitoring of ligand- and transducer-mediated GPCR conformational changes
Authors: Picard Louis-Philippe , Schönegge Anne Marie , Lohse Martin J. , Bouvier Michel
Published: August 2018

G protein-coupled receptors (GPCRs) are seven-transmembrane proteins that mediate a variety of cellular response which make them a target of choice for drug development in many indications. It is now well established that GPCRs can adopt several distinct conformations that can be differentially stabilized by various ligands resulting in different biological outcomes, a concept known as functional selectivity. However, due to the highly hydrophobic nature of GPCRs, tools to monitor these conformational ensembles are limited and addressing their conformation dynamics remains a challenge with current structural biology approaches. Here we describe new bioluminescent resonance energy transfer-based biosensors that can probe the conformational rearrangement promoted by ligands with different signaling efficacies as well as the impact of transducers such as G proteins and β-arrestin on these conformational transitions. The design of such sensors for other receptors should be useful to further explore the structural determinants of GPCR functional selectivity.
Dual-mode infrared laser-excited synergistic effect in NaGdF4:Er3+ nano-glass ceramics: a kinetic model
Authors: Wang Xiangfu , Wang Yemin , Bu Yanyan , Cheng Jie , Yan Xiaohong
Published: August 2018

The realization of the absorption and conversion of wide band infrared light have been a challenge in the field of upconversion luminescence.
Low-temperature solution-processed vanadium oxide as hole transport layer for efficient and stable perovskite solar cells
Authors: Guo Qiang , Wang Chenyun , Li Jinyan , Bai Yiming , Wang Fuzhi , Liu Lin , Zhang Bing , Hayat Tasawar , Alsaedi Ahmed , Tan Zhan’ao
Published: August 2018

Planar perovskite solar cells employing low-temperature-processed vanadium oxide as the hole transport layer with enhanced stability.
One step fabrication of novel Ag-CdS@EP floating photocatalyst for efficient degradation of organic pollutants under visible light illumination
Authors: Khan Usman Ali , Liu Jianjun , Pan Jinbo , Zuo Shengli , Ma Hecheng , Yu Yingchun , Ahmad Aftab , Ullah Sadeeq , Li Baoshan
Published: August 2018

In this paper, we present the fabrication of an expanded-perlite (EP)-based floating photocatalyst comprising CdS and Ag nanoparticles.
Efficient degradation of cytotoxic contaminants of emerging concern by UV/H2O2
Authors: Huang Ying , Liu Yiqing , Kong Minghao , Xu Elvis Genbo , Coffin Scott , Schlenk Daniel , Dionysiou Dionysios D.
Published: August 2018

The degradation kinetics and cytotoxicity of two commonly detected contaminants of emerging concern (CECs), diclofenac and triclosan, in UV/H2O2 system were investigated in this study.
A location- and sharpness-specific tactile electronic skin based on staircase-like nanowire patches
Authors: Gong Shu , Wang Yan , Yap Lim Wei , Ling Yunzhi , Zhao Yunmeng , Dong Dashen , Shi Qianqian , Liu Yiyi , Uddin Hemayet , Cheng Wenlong
Published: August 2018

A new stretchable tactile electronic skin sensor that could sense touch, location and sharpness is presented.
Nickel-catalyzed direct C–H bond sulfenylation of acylhydrazines
Authors: Li Jun-Ming , Yu Yang , Weng Jiang , Lu Gui
Published: August 2018

An efficient Ni-catalyzed direct C–H bond sulfenylation of acylhydrazines for the synthesis of diverse diaryl sulfides.
DFT exploration of [3 + 2] cycloaddition reaction of 1H-phosphorinium-3-olate and 1-methylphosphorinium-3-olate with methyl methacrylate
Authors: Hallooman Dhanashree , Ríos-Gutiérrez Mar , Rhyman Lydia , Alswaidan Ibrahim A. , Domingo Luis R. , Ramasami Ponnadurai
Published: August 2018

These phosphorus cycloadducts are kinetically and thermodynamically more favorable than their nitrogen analogues, providing incentives to experimentalists in the quest to synthesise phosphorus containing heterocycles.
Metal-free alkene oxy- and amino-perfluoroalkylations via carbocation formation by using perfluoro acid anhydrides: unique reactivity between styrenes and perfluoro diacyl peroxides
Authors: Valverde Elena , Kawamura Shintaro , Sekine Daisuke , Sodeoka Mikiko
Published: August 2018

A practical metal-free perfluoroalkylation using acid anhydrides with unique reaction mode via carbocation has been developed.
Enhanced thermoelectric performance of Sn-doped Cu3SbS4
Authors: Chen Kan , Di Paola Cono , Du Baoli , Zhang Ruizhi , Laricchia Savio , Bonini Nicola , Weber Cedric , Abrahams Isaac , Yan Haixue , Reece Mike
Published: August 2018

Enhanced thermoelectric performance of Cu3SbS4 with fine microstructure and optimized carrier concentration by Sn-doping.
Auditory Outcomes in Patients Who Received Proton Radiotherapy for Craniopharyngioma
Authors: Bass Johnnie K. , Huang Jie , Hua Chia-Ho , Bhagat Shaum P. , Mendel Lisa Lucks , Onar-Thomas Arzu , Indelicato Daniel J. , Merchant Thomas E.
Published: August 2018

Purpose

Compared to photon-based radiotherapy, protons deliver less radiation to healthy tissue resulting in the potential reduction of late complications such as sensorineural hearing loss (SNHL). We report early auditory outcomes in children treated with proton radiotherapy (PRT) for craniopharyngioma.

Method

Conventional frequency (CF = 0.25–8.0 kHz) audiometry, extended high-frequency (EHF = 9.0–16.0 kHz) audiometry, distortion product otoacoustic emission (DPOAE) testing, and speech-in-noise (SIN) assessments were prospectively and longitudinally conducted on 74 children with a median of 2 post-PRT evaluations (range, 1–5) per patient. The median age at PRT initiation was 10 years, and median follow-up time was 2 years. Ototoxicity was classified using the Chang Ototoxicity Grading Scale (Chang & Chinosornvatana, 2010) and the American Speech-Language-Hearing Association (ASHA) criteria (ASHA, 1994). Comparisons were made between baseline and most recent DPOAE levels, with evidence of ototoxicity based on criterion reductions of ≥ 6 dB. The critical difference values for comparing SIN scores between two conditions (i.e., pre- and post-PRT) were used to determine a significant change between test scores.

Results

At last evaluation, no patients had SNHL in the CF range, and 2 patients had SNHL (Chang Grade 1a) in the EHF range. Based on the ASHA criteria, a decrease in hearing was observed in 0 patients in the CF range alone, in 9 patients in the EHF range alone, and in 15 patients in both the CF and EHF ranges. DPOAE levels decreased at a faster rate at higher versus lower frequencies. For 41 evaluable patients, SIN perception did not decline over time (
p
= .6463).

Conclusion

At a median follow-up time of 2 years post-PRT, normal hearing was maintained within the CF range. However, subclinical decreases in hearing were observed, particularly in the EHF range and in the DPOAE level; thus, long-term follow-up is recommended to monitor for potential auditory late effects from PRT.
Testing the Hyperarticulation and Prosodic Hypotheses of Child-Directed Speech: Insights From the Perceptual and Acoustic Characteristics of Child-Directed Cantonese Tones
Authors: Wong Puisan , Ng Kelly Wing Sum
Published: August 2018

Purpose

The function of child-directed speech has been debated for decades. This study examined the perceptual and acoustic characteristics of child- and adult-directed Cantonese tones to test the hyperarticulation and prosodic hypotheses that have been proposed to account for the acoustic modifications in child-directed speech.

Method

Sixty-two mother–child dyads participated in the study. The mothers verbally labeled 30 pictures in monosyllabic isolated words and in the final position of a carrier sentence to the experimenter and their 1- to 5-year-old children. The 8,634 adult- and child-directed productions were low-pass filtered to eliminate lexical information and presented to 5 judges for tone identification. Acoustic analysis was performed on the productions.

Results

Acoustically, child-directed tones were produced with an elevated pitch, and the pitch level decreased as the child's age increased. Acoustic contrasts between phonetically similar and more confusing tones were not enhanced in child-directed speech, and unexpectedly, child-directed tones were identified with a lower accuracy than adult-directed tones. The perceptual errors of child-directed tones mirrored the errors found in identifying tones excised from sentence-final position, which had a pitch-lowering effect on the tones. The lower perceptual accuracy, the lack of enhanced acoustic contrasts in confusing tone pairs, and the similarities in the error patterns in identifying tones in child-directed speech and tones in utterance-final position suggest that the acoustic modifications in child-directed tones are prosodic effects serving pragmatic purposes.

Conclusion

The findings reject the hyperarticulation hypothesis and support the prosodic hypothesis of child-directed speech.
Real‐time detection of distracted driving based on deep learning
Authors: Tran Duy , Manh Do Ha , Sheng Weihua , Bai He , Chowdhary Girish
Published: August 2018

Driver distraction is a leading factor in car crashes. With a goal to reduce traffic accidents and improve transportation safety, this study proposes a driver distraction detection system which identifies various types of distractions through a camera observing the driver. An assisted driving testbed is developed for the purpose of creating realistic driving experiences and validating the distraction detection algorithms. The authors collected a dataset which consists of images of the drivers in both normal and distracted driving postures. Four deep convolutional neural networks including VGG‐16, AlexNet, GoogleNet, and residual network are implemented and evaluated on an embedded graphic processing unit platform. In addition, they developed a conversational warning system that alerts the driver in real‐time when he/she does not focus on the driving task. Experimental results show that the proposed approach outperforms the baseline one which has only 256 neurons in the fully‐connected layers. Furthermore, the results indicate that the GoogleNet is the best model out of the four for distraction detection in the driving simulator testbed.
Wideband high‐gain millimetre/submillimetre wave antenna using additive manufacturing
Authors: Gu Chao , Gao Steven , Sanz Izquierdo Benito , Gibbons Gregory J. , Young Paul R. , Parker Edward A. , Qin Fan , Wen Geyi , Cheng Zhiqun , Geng Youlin , Liu Ying
Published: August 2018

This study presents a novel design of a wideband high‐gain resonant cavity antenna (RCA) for millimetre and submillimetre wave bands, and its fabrication using additive manufacturing (AM). The proposed RCA antenna consists of a partially reflecting surface and three impedance matching layers fed by a waveguide. AM techniques are utilised to fabricate the design operating at 30 GHz. Two fabrication techniques are assessed for printing the antenna. The first technique is based on printing a dielectric material and fully coating the parts with a metallic layer, while the second technique involves printing the parts in a single process using metal three‐dimensional printing. The first technique offers a lightweight solution while the second technique can print the whole model in one run. The antenna design is investigated by both simulations and experiments. The measured results show a 3 dB gain bandwidth of about 10%, and high gain over 15 dBi for all the three resulting antennas. Good agreement between simulation and measurement is obtained. The antenna is of low cost and achieved good performance in terms of wide bandwidth and high gain, thus it is potentially useful for high‐speed wireless communications at millimetre‐wave and sub‐millimetre‐wave frequencies.
Review of radar classification and RCS characterisation techniques for small UAVs or drones
Authors: Patel Jarez S. , Fioranelli Francesco , Anderson David
Published: August 2018

This review explores radar‐based techniques currently utilised in the literature to monitor small unmanned aerial vehicle (UAV) or drones; several challenges have arisen due to their rapid emergence and commercialisation within the mass market. The potential security threats posed by these systems are collectively presented and the legal issues surrounding their successful integration are briefly outlined. Key difficulties involved in the identification and hence tracking of these ‘radar elusive’ systems are discussed, along with how research efforts relating to drone detection, classification and radar cross section (RCS) characterisation are being directed in order to address this emerging challenge. Such methods are thoroughly analysed and critiqued; finally, an overall picture of the field in its current state is painted, alongside scope for future work over a broad spectrum.
The CARMENES search for exoplanets around M dwarfs
Authors: Kaminski A. , Trifonov T. , Caballero J. A. , Quirrenbach A. , Ribas I. , Reiners A. , Amado P. J. , Zechmeister M. , Dreizler S. , Perger M. , Tal-Or L. , Bonfils X. , Mayor M. , Astudillo-Defru N. , Bauer F. F. , Béjar V. J. S. , Cifuentes C. , Colomé J. , Cortés-Contreras M. , Delfosse X. , Díez-Alonso E. , Forveille T. , Guenther E. W. , Hatzes A. P. , Henning Th. , Jeffers S. V. , Kürster M. , Lafarga M. , Luque R. , Mandel H. , Montes D. , Morales J. C. , Passegger V. M. , Pedraz S. , Reffert S. , Sadegi S. , Schweitzer A. , Seifert W. , Stahl O. , Udry S.
Published: August 2018

Despite their activity, low-mass stars are of particular importance for the search of exoplanets by the means of Doppler spectroscopy, as planets with lower masses become detectable. We report on the discovery of a planetary companion around HD 180617, a bright (J = 5.58 mag), low-mass (M = 0.45M⊙) star of spectral type M2.5 V. The star, located at a distance of 5.9 pc, is the primary of the high proper motion binary system containing vB 10, a star with one of the lowest masses known in most of the twentieth century. Our analysis is based on new radial velocity (RV) measurements made at red-optical wavelengths provided by the high-precision spectrograph CARMENES, which was designed to carry out a survey for Earth-like planets around M dwarfs. The available CARMENES data are augmented by archival Doppler measurements from HIRES and HARPS. Altogether, the RVs span more than 16 yr. The modeling of the RV variations, with a semi-amplitude of K = 2.85-0.25+0.16 m s-1, yields a Neptune-like planet with a minimum mass of 12.2-1.4+1.0 M⊕ on a 105.90-0.10+0.09 d circumprimary orbit, which is partly located in the host star’s habitable zone. The analysis of time series of common activity indicators does not show any dependence on the detected RV signal. The discovery of HD 180617 b not only adds information to a currently hardly filled region of the mass-period diagram of exoplanets around M dwarfs, but the investigated system becomes the third known binary consisting of M dwarfs and hosting an exoplanet in an S-type configuration. Its proximity makes it an attractive candidate for future studies.
XZ: Deriving redshifts from X-ray spectra of obscured AGN
Authors: Simmonds C. , Buchner J. , Salvato M. , Hsu L.-T. , Bauer F. E.
Published: August 2018

Context. Redshifts are fundamental for our understanding of extragalactic X-ray sources. Ambiguous counterpart associations, expensive optical spectroscopy, and/or multimission multiwavelength coverage to resolve degeneracies often make estimation difficult in practice.

Aims. We attempt to constrain redshifts of obscured active galactic nuclei (AGN) using only low-resolution X-ray spectra.

Methods. Our method for determining redshifts from the X-ray spectrum (XZ) fits AGN X-ray spectra with a moderately complex spectral model incorporating a corona, a torus obscurer, and a warm mirror. Using the Bayesian X-ray Astronomy (BXA) package, we constrain redshift, column density, photon index, and luminosity simultaneously. The redshift information primarily comes from absorption edges in Compton-thin AGN, and from the Fe Kα fluorescent line in heavily obscured AGN. A new generic background fitting method allows us to extract more information from limited numbers of source counts.

Results. We derive redshift constraints for 74/321 hard-band detected sources in the Chandra deep field South. Comparing with spectroscopic redshifts, we find an outlier fraction of 8%, indicating that our model assumptions are valid. For three Chandra deep fields, we release our XZ redshift estimates.

Conclusions. The independent XZ estimate is easy to apply and effective for a large fraction of obscured AGN in today’s deep surveys without the need for any additional data. Compared to different redshift estimation methods, XZ can resolve degeneracies in photometric redshifts, help detect potential association problems, and confirm uncertain single-line spectroscopic redshifts. With high spectral resolution and a large collecting area, this technique will be highly effective for Athena/WFI observations.
Identification and Expression of vitellogenin gene in the Gouramy (Osphronemous Gourammy) under photoperiods manipulation
Authors: Prayogo Norman Arie , Siregar Asrul Sahri , Sukardi Purnama , Nugrayani Dewi , Ekasanti Anandita , Yasumasa Bessho Riviani
Published: August 2018

Vitellogenin was protein hormone where controlled gonad development in fish. Expression of this gene regulated from the external and internal factor.Photoperiods are the external factor that regulated endocrine gland activities in gonadal development, gametogenesis and reproductive cycles. Aim of the research to find out the effect of photoperiod on gouramy reproductive performance by manipulating photoperiod. Design experimental with three photoperiod treatments, namely 14L: 10D (control), 8L: 16D (short photoperiods) and 18L: 6D (long photoperiods). Four aquaria consisting of nine fishes each were served as replicates. Fishes were kept under these photoperiods for 8 weeks. The observed variable was the liver activities. Liver activity was evaluated by measuring gene expression of Vitellogenin. The normalized data were subjected to ANOVA followed by Tukey’s multiple-comparison tests. The length of Vitellogenin cDNA was 1136 bp. The vitellogenin precursors encoded cDNA consisted of 378 amino acids. The average of vitellogenin gene in each experimental group significantly increased according to longer photoperiods (P<0.05). These results indicated that photoperiods had a stimulatory effect in improving gouramy reproductive performance
Rearing technology for climbing fish (Anabas testudineus) on different salinity
Authors: Widiyati Ani , Asyiah B.N. , Heru Prihadi Tri , Puspaningsih Dewi
Published: August 2018

Optimal salinity on rearing media is needed to get the best production performance of climbing fish culture. The aim of this experiment is to get an optimal salinity on rearing media of climbing fish. This experiment was conducted in wet lab of Research Institute for freshwater Aquaculture and Fisheries Extension. The treatments were different salinities on rearing media; 0 ppt, 3 ppt, 6 ppt and 9 ppt. Each treatment has 3 replicates. Experiment design use completely randomized design. The initial weight of fish was 1 ± 0.11g and initial length of fish was 2 ± 0.3 cm. Aquaria of 40x22x20 cm was used for rearing media. Each aquarium with 15 lt of water was stocked 350 fish. Fish was fed frozen blood worm (protein contains of 62.5%) at ad libitum. Duration time of rearing was 40 days. Sampling was conducted every 10 day for fish weight, lenght and water quality. The result showed that optimal salinity of survivale rate, specific growth rate and total length were on salinity of 1.8 ppt, 2.5 ppt and 3.3 ppt. The value of water quality parameter in aquaria such as temperature, pH, Oxygen, nitrite, nitrate an amonia is still feasible for climbing fish rearing.
Polyculture of Giant Freshwater Prawn, Macrobrachium rosenbergii and Nilem Carp, Osteochilus hasselti Cultured in Recirculation System Using Biofiltration
Authors: Setiadi Eri , Taufik Imam
Published: August 2018

Giant Freshwater Prawn is one of the freshwater commodity which have an economy value where Nilem carp has also potentially to be cultured to produce fingerling size as a “baby fish”. The purpose of this experiment is to determine the optimal survival, growth, FCR, and productivity of Giant Freshwater Prawn and Nilem carp cultured on polyculture system and also biomass productivity of bio filter (snail, water spinach, and Cabomba). The different stocking density of Nilem carp as a treatments were as followed: A) 25 fish m-3 B) 50 fish m-3, and C) 75 fish m-3. Density of Giant Freshwater Prawn was 20 individuals m-3 for all treatments. The result showed that the optimal of survival, growth (length and weight), productivity and FCR for Giant Freshwater Prawn was found at density of 25 fish m-3 (A) than that of density 50 fish m-3 (B) and 75 fish m-3 (C) (P<0.05). The highest of biomass productivity of bio filter (snail, water spinach, and Cabomba) was found at density of 25 fish m-3 (A) than that of density of 50 fish m-3 (B) and 75 fish m-3 (P<0.05). Water quality such as DO, temperature and pH showed almost the same at all treatments.
Sublethal Toxicity Tests of Mercury (Hg) to Nilem Fish (Osteochilus hasselti) Gills Tissue Damage
Authors: Sahri Siregar Asrul , Arie Prayogo Norman , Listiowati Emyliana , Santoso Mahendro , Gumay Yudha Indra , Wahyuni Sholehah Tri
Published: August 2018

Industry development in Indonesia resulted in sewage and polluting heavy metals such as mercury (Hg). These pollutants will be harm organisms that live at aquatic environment. Fish gills could be damage due to exposure by mercury. To know the influence of mercury’s toxic against aquatic organisms by using toxicity test. An aquatic organism, which is usually used as toxicity test, is nilem fish (Osteochilus hasselti). The objective of the study was to determine the value of LC50-96 hours in a toxicity test of nilem fish exposed to lead acetate; to determine the changes in the fish gill and to determine the concentration of lead mercury which causes tissue damage structure in the gills of nilem fish. The design used completely randomized design (CRD) with four concentrations (0; 0.08; 0.16 and 0.24 ppm) and three replications. The objective of the study was sublethal toxicity test. Lethal toxicity concentration data were descriptive and data from sublethal toxicity test were One Way. The analysis showed that there were highest edema 10% and 17% on day 4 and 12, the highest lamella fusion 35% and 59% on day 4 and 12, the highest hyperplasia 45% and 54% on day 4 and 12, and the highest necrosis the 65% and 80% on day 4 and 12. It was concluded that the value of LC50-96 hours in nilem fish (Osteochilus hasselti) toxicity test was 0,3938 ppm; and damage of fish gill tissue changes started to occur in the lead mercury (Hg) concentration of 0,08 ppm.
Acute effects of crude oilfor three common mangrove seedling in segara anakan nature reserve (sanr) cilacap, Indonesia
Authors: Harisam Taufan , Sugiyono  , Pribadi Rudhi , Sari Siregar Asrul , Wisudyanti Budi Hastuti Dewi , Arie Prayogo Norman
Published: August 2018

The growth of three mangrove species seedlings,Bruguiera gymnorrhiza, Rhizophora apiculataand Ceriops decandra in sediments contaminated by crude oil. Thisresearchwas designed to evaluate the acute effects of crude oil on the growth and survival performance of mangrove seedling in 4-week field experiment. Three treatments(sub plot) were compared with the control (no oil added) usingi.e. 100 ml, 120 ml and 140 ml crude oil applied once, in a split-plot design with nine replicates. The result showed the extent of survival has maximum for B. gymnorrhiza while it was minimum for C. decandra. The growth of mangrove seedlings was decrease with higher concentrations of crude oil. Stem height of B. gymnorrhiza seedling higher than R. apiculata and C. decandra.We observed, severe damage in the epidermis and the cortex at the root of all three types of mangrove seedlings when crude oil applied at 140 ml.
Occurrence of beach debris in Tunda Island, Banten, Indonesia
Authors: Maharani Adinda , Purba Noir P , Faizal Ibnu
Published: August 2018

Marine Debris is one of the spotlight issues of the world. The existence of marine debris can disrupt the ecosystem. Marine Debris carried by the current to coastal areas and accumulated along the coast. The study area of this research was on the coastline of TundaIsland, which located between Ban-ten and Lampung Provinces. These two provinces are part of two major islands of Indonesia, Java and Sumatra Island which have a high density of population. Household needs produce every day directly proportional to the population. This study is to record what types of garbage contained along the coast. There are 8 stations used in this study using International Coastal Cleanup (ICC) form with an area of 100 m2 collection at each station. From 800m2 area coverage, 1,234 items collected with 47 kg weight of debris and the density 0.072 kg/m2 with abundance 0.75 items/m2. This result suggests that household needs give the big proportion of debris such as Food wrapper, cigarettes butts, and foam pieces. In addition, the study indicates that there is a strong relation between human activities and spreading of debris.
Harmonization of regulation in water territorial management becoming a fair economic benefit distribution towards regional autonomy
Authors: Sudrajat Tedi , Raharjo Agus , Wasi Bintoro Rahadi , Saefudin Yusuf
Published: August 2018

The extent of Indonesian territorial waters along with its natural wealth inside brings economic benefit, yet on the other side it invites problems. Especially with the existence of regional autonomy, the competition to obtain the economic benefit causes fiercer competition between regions. The competition causes the division emergence of marine areas which implicates towards the fate of fishermen. This relates to their catchment area, imposition of income tax, and technical restriction on fishing. This research used normative approach by emphasizing the comparative study of water territorial arrangement in various regions. Based on the research, in autonomy region which has marine water, they regulate the object very detail and there are some which exploit their area. The detailed and thorough regulation with its practice sometimes cause the territorial waters dispute among the regions. It causes a confusion for the government or fishermen in obtaining the economic benefit of their own water. Hence, in level of regulation, it needs a legal harmonization between autonomy regions in utilization of water territorial. In practical level it often needs coordination to create fair economic benefit for the stakeholders.
The Roles of the Ministry of Marine and Fishery in Marine Environment Protection from Oil Pollution by Tankers in the Sea in Indonesian Legal System
Authors: Purwendah Elly Kristiani , Triatmodjo Marsudi , Sulistiawati Linda Yanti
Published: August 2018

The Ministry of Marine and Fishery (KKP), as stipulated in the Presidential Regulation Number 2 of 2017 on the Amendment of the Presidential Regulation Number 63 of 2015 on the Ministry of Marine and Fishery, is in charge of organizing government affairs in the field of marine and fisheries to assist the President in organizing the state government. In one of the seven pillars of Indonesia's marine policy, which is in the pillars of the management in the scope and protection of marine environment, one of the major programs of marine environment protection policy is the prevention, mitigation, and recovery of pollution impacts and damage to marine environment. For the achievement of the objectives, the KKP requires a normative support for the protection to marine environment from oil pollution by tankers, in terms of prevention, mitigation, and recovery of pollution impacts and damage to marine environment.
Prospect on Implementation of National Fish Logistics System: case in Indonesia
Authors: Azhar Muhamad , Suhartoyo Suhartoyo , Suharso Putut , Herawati Vivi Endar , Trihastuti Nanik
Published: August 2018

The policy to realize the sustainable supply of fish (especially marine fish commodities), face the fundamental constraints of seasonal factors, remoteness and characteristic of damaged fish commodities. At the time or harvest season, the production of catch fish can be abundant, but in the famine season, there is the opposite condition. Meanwhile, on the consumer (community) and industry side, fish supply must be available all the time without getting to know the season in constant amount. The research aims to find out the prospect of implementation of national fish logistic system or Sistem Logistik Ikan Nasional (SLIN), especially in Indonesia. This research is an empirical juridical research using statutory uproach approach, conceptual aproach, and comparative approach. Then analyzed using synthesis analysis. The results showed that SLIN implemetasi provide great benefits for national fish production, also directly contribute to the increase of national economic development. SLIN Management can run optimally if there is a synergy between all entities involved in each activity from upstream (production side) to downstream (consumption side). In the framework of looking at best practices for upstream downstream supply chain management of fishery commodities as well as in accordance with the mandate of Minister of Maritime Affairs and Fisheries No. 05/Permen-KP/2014 on SLIN. Thus, the Directorate General of Processing and Marketing of Fishery Products sees the importance of better implementation of SLIN. In the Initial Stage by providing several supporting facilities and involving the main operators and supporting operators in the management of selected locations.
Practices of Illegal Fishing in Pemalang Region: A Policy Analysis
Authors: Wahyuningrat  , Haryanto Tenang , Rosyadi Slamet
Published: August 2018

The use of unsustainable fishing gear has resulted in the decline of fish resources and threatens the sustainability of fish resources. This can cause harm to the state and the interests of the fishermen themselves. Although various policies governing fishing gear are already in place, unsustainable fishing practices remain. This, of course, has resulted in overfishing and causing environmental damage. Using the case of fishing practices in Pemalang Region, this study found that fishing gears used by fishermen are prohibited tools because they have negative impacts on the environment. Some fishing gear is quite popular used by fishermen in Pemalang Region are stun, fishing nets, fishing rods and poison. Unfortunately, the use of electric current tool (stun) is found to be more dominant than fishing nets and fishing rods. Similarly, it has developed among fishermen to use toxic methods in fishing. This phenomenon encourages researchers to formulate an effective public policy to control and minimize illegal fishing practices.
Sustainability of Seahorses: Lessons Learned of Local Wisdom from Bintan Islands, Riau Islands Province
Authors: Ulfah Fitria , Afrizal  , Pratomo Arief
Published: August 2018

Seahorse (Hippocampus spp.) has a high economic value in Bintan Island that leads to its exploitation by coastal community intensively. This study was to assess local knowledge on seahorse ecology which was captured across shoreline near the coastal communities. The research was conducted in SebongPereh village Bintan Island. The data were collected by conducting depth interviews and focus group discussion on seahorse fishermen which was selected purposively. The results revealed local fishermen have ecological knowledge related to habitat types, behavior, and monthly variation in abundance, size and reproduction pattern. The result also showed that the existence of the seahorse during the catch season moves around, follow the condition of the seaweed and tidal of sea water. This local knowledge was used by fishermen as a strategy in determining the fishing time and catchment area of a seahorse. Through such local wisdom, local fishing communities established a local institution to manage conversation and sustainability use of seahorse in waters of SebongPereh village.
Revisiting the passivation of stainless steels and other passive CRAs
Authors: Crolet Jean- Louis
Published: August 2018

All that was said so far about passivity and passivation was indeed based on electrochemical prejudgments, and all based on unverified postulates. However, due the authors’ fame and for lack of anything better, the great many contradictions were carefully ignored. However, when resuming from raw experimental facts and the present general knowledge, it now appears that passivation always begins by the precipitation of a metallic hydroxide gel. Therefore, all the protectiveness mechanisms already known for porous corrosion layers apply, so that this outstanding protectiveness is indeed governed by the chemistry of transport processes throughout the entrapped water. For Al type passivation, the base metal ions only have deep and complete electronic shells, which precludes any electronic conductivity. Then protectiveness can only arise from gel thickening and densification. For Fe type passivation, an incomplete shell of superficial 3d electrons allows an early metallic or semimetallic conductivity in the gel skeleton, at the onset of the very first perfectly ordered inorganic polymers (- MII-O-MIII-O-)n. Then all depends on the acquisition, maintenance or loss of a sufficient electrical conductivity in this Faraday cage. But for both types of passive layers, all the known features can be explained by the chemistry of transport processes, with neither exception nor contradiction.
La répression catabolique ou comment les bactéries choisissent leurs sucres préférés
Authors: Galinier Anne
Published: August 2018

La répression catabolique permet aux bactéries, mais aussi aux levures ou champignons, une utilisation préférentielle des sources de carbone. Ce phénomène se traduit par une croissance diauxique durant laquelle les bactéries assimilent d’abord les sources de carbone rapidement métabolisables, puis les sources de carbone non préférentielles. Divers mécanismes moléculaires sont responsables de la répression catabolique et contrôlent non seulement l’expression de gènes impliqués dans l’utilisation de sources de carbone alternatives, mais aussi l’expression de plusieurs gènes impliqués dans des processus cellulaires variés. Cette synthèse décrit les principaux mécanismes moléculaires retrouvés chez les entérobactéries et chez les firmicutes, ainsi que l’importance du système des phosphotransférases dans cette régulation.
Lymphocytes NK : un rôle majeur dans le contrôle immunologique de la leucémie myéloïde chronique
Authors: Toubert Antoine , Turhan Ali , Guerci-Bresler Agnès , Dulphy Nicolas , Réa Delphine
Published: August 2018

Les dernières études menées sur la leucémie myéloïde chronique (LMC) ont permis de mettre en évidence le rôle clé des cellules NK (natural killer) dans cette maladie avec trois enseignements majeurs : (1) au diagnostic, les cellules NK sont anormales dans leurs propriétés phénotypiques et fonctionnelles ; (2) un traitement standard par inhibiteur de tyrosine kinase (ITK) est associé à la correction de certaines anomalies, en particulier celles des lymphocytes NK ; (3) le taux du sous-type mature NK CD56dim pourrait être un facteur du maintien d’une survie sans rechute après une stratégie d’arrêt de traitement par ITK. Ces données renforcent le rôle des lymphocytes NK dans le contrôle immunologique de la LMC.
Au cœur de la cardiomyopathie diabétique
Authors: Lugat Alexandre , Joubert Michael , Cariou Bertrand , Prieur Xavier
Published: August 2018

Le diabète de type 2 (DT2) est un facteur de risque indépendant de l’insuffisance cardiaque. Ces anomalies sont cependant associées à une lipotoxicité et à une glucotoxicité cardiaques. Pourtant, les mécanismes exacts de ces toxicités cardiaques demeurent inconnus. Récemment, le phénotype cardiaque d’un modèle unique de DT2, les souris lipodystrophiques invalidées pour le gène codant la seipine (SKO), a révélé l’importance de la glucotoxicité dans le développement des anomalies cardiaques. En effet, les souris SKO présentent une cardiomyopathie associée à une surcharge en glucose, qui est corrigée par l’utilisation d’un agent hypoglycémiant, un inhibiteur du co-transporteur sodium-glucose de type 2 (SGLT2). Dans ce modèle, la glucotoxicité peut ainsi, à elle seule, générer les dysfonctions cardiaques associées au diabète.