bluuebunny/crossref_metadata_2025 huggingface dataset:
Pemetaan Distribusi Ekosistem Mangrove di Wilayah Kota Surabaya dan Sidoarjo Memanfaatkan Citra Landsat TM-5
[Distribution Mapping of Mangrove Ecosystem in Surabaya and Sidoarjo by Using Landsat TM-5 Imagery]
Authors: Hidayah Zainul
Published: February 2019

Abstract Rapid developments on the coastal regions have become a major thread to mangrove ecosystems. The conversion of mangrove forest into fish ponds, housing and industrial sites make the area of this unique tropical ecosystem decline significantly in the last decade. To prevent further destruction of mangrove ecosystems due to human activities, conservation programs are needed. Therefore, information on mangrove's area as well as their distribution is needed. The main objective of this research was to demonstrate the ability of remote sensing and geographic information technology to provide reliable and accurate data on mangrove forest in Surabaya and thSidoarjo regions. A Landsat TM-5 imagery (acquisition date June 4 , 2009) data was utilized to produce the map. The results showed that in the 2009 period the area of mangrove forest in Surabaya and Sidoarjo was 378.19 Ha and 1236.42 Ha respectively. However, over 73.5% area of mangrove in Surabaya and 43.25% in Surabaya were under critical conditions. Anthropogenic factors and human influence were some of the main factors that cause the condition. Activities such as illegal and uncontrolled logging, conversion of mangrove's area and the lack of people's awareness in the importance of mangrove ecosystems were also problems that lead to the massive damage of mangrove forests.
Pemberian Perasan Buah Mengkudu (Morinda citrifolia) untuk Pengendalian Argulus Pada Ikan Mas Komet (Carassius auratus auratus)
[Giving Morinda Fruit Distilation (Morinda citrifolia) For Argulus Handling On Carrasius auratus auratus]
Authors: Kismiyati Kismiyati , Ghazali Iqbal , Mahasri Gunanti
Published: February 2019

Abstract This study aims to determine the effect of giving Morinda fruit distilation for handling Argulus on Carrasius auratus auratus. The research method that used was experimentally with Completely Randomized Design (CRD) with five treatments and four replications. The used treatment are : medium with Morinda distilation mixed 0% (A), medium with Morinda distilation mixed 2,5% (B), medium with Morinda distilation mixed 3% (C), medium with Morinda distilation mixed 3,5% (D), medium with Morinda distilation mixed 4% (E). The results showed that giving Morinda fruit distillation on Carrasius auratus auratus which have Argulus infest significantly different (p <0.05) with the best treatment in D with six releasing Argulus and that fish can survive within 15 minutes dipping. The lowest treatment result in A (control) with nothing releasing Argulus. Water quality parameters are supporting this research. Supporting parameters measured during the study is the water temperature ranges 27° C, pH 7,5-8,5, DO 8 mg/L to 5 mg/L, and salinity from 0 to 3 ppt. Water quality parameter are still within tolerance limit for Carrasius auratus auratus
Kandungan Protein Kasar dan Serat Kasar Pada Kulit Pisang Raja yang Difermentasi dengan Trichoderma viride dan Bacillus subtilis sebagai Bahan Baku Pakan Ikan
[ Crude Protein And Fiber Content Of Rough Of Fermented On The King Banana Skins By Trichoderma viride And Bacillus subtilis As Raw Fish Feed]
Authors: Agustono Agustono , Yusuf Mohamad , Meles Dewa Ketut
Published: February 2019

Abstract Utilization of fish waste as an alternative feed ingredient in the effort to increase the availability of raw materials making up rations. Banana skin is one that has the potential of agricultural waste into alternative feed ingredients, but the nutritional value contained in the banana peel is still low so we need to attempt to improve nutritional content, one of which is fermentation method using Trichoderma viride and Bacillus subtilis in order to improve crude protein and lower content of crude fiber on a banana peel. This study aims to determine the influence of Trichoderma viride on crude protein and crude fiber are fermented banana skin, know the effect of Bacillus subtilis on crude protein and crude fiber are fermented banana skin and know the effect of interaction of Trichoderma viride and Bacillus subtilis of crude protein and fiber fermented banana skin rough. This research method is to experiment with Completely Randomized Design (CRD) as a factorial experimental design. The treatments used consisted of two factors, namely the effect of Trichoderma viride (factor A) and Bacillus subtilis (factor B). Factor A consisted of three dose levels: 0%, 3% and 6%, while factor B consists of three levels ie dose of 0%, 4% and 8%. Each treatment combination was replicated 3 times. The results showed that the use of Trichoderma viride and Bacillus subtilis in fermentation processes plantain skin showed significant differences on crude protein and crude fiber on dry matter (p<0.05).
Prakiraan Kesuburan Perairan Bali Dari Citra Satelit
[Forecast Fertility Bali Waters From Satellite Imagery]
Authors: Abdul Manan Sony Angga Satrya,
Published: February 2019

Abstract Aplication Ocean Remote Sensing technology to many use for field fisheries, once use this technology in forecast fertility water. The aim of this study was to determine the feasibility of Bali coastal area for cage of pearl oyster culture. The method used is a descriptive method of data collection. Satellite image processing activities Aqua/Terra Modis starting with the collection of satellite image data from the database NASA via OceanColor Web site, the selection of a clean image data, and than download of satellite images. The first stages of image data processing are used software ENVI 4.7, with procedures are coloring the image, limiting the minimum and maximum temperatures and sea surface chlorophyll-a, and classifiying of the image based on the value of sea surface temperature. Sea surface temperature parameter determine the location of the cage of pearl oysters (Pinctada maxima) culture. Suitability of the location of the cage of pearl oyster culture on Bali coastal area, at coordinates 8° 33' 00.97 " - 8° 42' 05.30" South Latitude and 115° 18' 03.40 " - 115° 39 ' 03.21" East Longitude. Based on geographical, that the location in the southeastern Bali coastal area and on the northern area of Nusa Pennida island.
Uji Sensitifitas Sari Buah Pare (Momordica charantia L) pada Bakteri Edwardsiella tarda dengan Metode Difusi Kertas Cakram Secara In Vitro
[Sensitivity Test Of Fruit Bitter Melon Juice (Momordica charantia L) On Bacteria Edwardsiella tarda With Paper Disc Diffusion Method In Vitro]
Authors: Sudarno Sudarno , Rosanti Selvi Lely , Subekti Sri
Published: February 2019

Abstract One of the problems encountered in fish farming is the disease attack. The attack of disease that was caused by bacteria is a problem that often occur in aquaculture. Traditional medicine is more readily accepted by communities because is cheap and easily available, have been quite familiar with the community. There were various kind of traditional medicine and has been widely studied chemically content and usefulness. Therefore there were still many plants unknown levels of toxicity, therefore need further study. Bacteria E. tarda spread in some countries such as Europe, Japan, Taiwan, Thailand, United States, Singapore, and Malaysia. While in Indonesia E. tarda were found in Java, Sumatra, and Borneo. The aim of the research is to know the capabilities of the inhibition zone of fruit bitter melon power against the bacteria E. tarda in vitro and knowing whether fruit bitter melon effective in inhibiting the growth of the bacteria E. tarda with gold standard comparator antibiotics Tetracycline. The research was conducted on February 2011 in Bacteriology and Microbiology, laboratory in Fish Quarantine Juanda Sidoarjo district of East Java. The method used in this research is descriptive method that was done in vitro by using sensitivity test of diffusion method with the determination of inhibition zone by comparing with antibiotic inhibition zones. The results showed that bitter melon juice has a bacterial growth inhibition against E. tarda, although when was compared with gold standard antibiotic tetracycline shared considered less sensitive. Observations showed clear zone of bitter melon fruit juice shared with a diameter of 13.3mm, was compared with standard antibiotic tetracycline can inhibit with a minimum diameter of 15mm. Sensitivity test of bitter melon fruit juice will be classified as not susceptible to the bacteria therefore is able to inhibit E. tarda..
Efektivitas Perasan Asam Jawa (Tamarindus indica L) Terhadap Pertumbuhan Bakteri Aeromonas hydrophila dengan Metode Difusi Kertas Cakram
[Effectiveness Of Thamarin Juice (Tamarindus indica L) On Growth Of Bacteria Aeromonas hydrophila With Paper Disc Diffusion Method]
Authors: Sulmartiwi Laksmi , Chrisanti Rizky Aprilia , Prayogo Prayogo
Published: February 2019

Abstract Fish disease is one of the serious problems that faced by the farmers potentially to make high disadvantages as follows, mortality of fish and fish quality decrease, therefore economically will caused price decrease. Bacterial pathogens were one of the problems that were faced by fish farmers, which cause 90% of mortality. Diseases control caused by Aeromonas hydrophila in general using by antibiotics. Antibiotics are commonly used were tetracycline, oxolinic acid, erytromicin, streptomycin, and chloramphenicol. Using of antibiotics has shown good results, but on the other hand using of antibiotics or antibacterial cultivation would increased the bacteria number of resistant to antibiotics. This research was purposed to find the alternative treatment of Motile Aeromonas Septicemia by determination ability of a squeeze of tamarind (Tamarindus indica L) and the minimum concentration of tamarind (Tamarindus indica L) to inhibit the growth of Aeromonas hydrophila. The function of tamarind as an antibacterial because they contain antibacterial substance such as flavonoids and saponins. The results showed that the antibacterial power of tamarind juice has antibacterial power against Aeromonas hydrophila. Based on a sentsitivity test was performed according to standard of antibiotic concentrations that are common to Aeromonas hydrophila, was Tetracycline inhibition zone with <15 mm diameter (not sensitive), 15-18 mm (moderately susceptible), >18 mm (very sensitive) is A (10%), and B (12.5%), treatment inhibition zone subsequently 13 mm and 14.3 mm (insensitive), C (15%), D (17.5%), with inhibition zone 15.5 mm and 16.6 mm (self-sensitive) and 20% with inhibition zone diameter of 19 mm (highly sensitive). Therefore, it was concluded that A treatment (15%) with inhibition zone 15.5 mm showed the inhibition ability of Aeromonas hydrophila growth.
Pengaruh Penambahan Atraktan yang Berbeda dalam Pakan Pasta Terhadap Retensi Protein, Lemak dan Energi Benih Ikan Sidat (Anguilla bicolor) Stadia Elver
[ Effects Of Addition Different Attractants In Pasta Feed Against Retention Of Protein, Fat and Energy Eel Fish Seed (Anguilla bicolor) Stadia Elver]
Authors: Arief Muhammad , Yudiarto Suryo , Agustono Agustono
Published: February 2019

Abstract Indonesia has a great potential for the development of eel fish (Anguilla bicolor) farming. Availability of seeds is not enough to make the farmers are interested to cultivate eel fish because fish growth is long enough. It can be addressed through accelerated consumption of feed with the proper administration of feed attractants that can spur growth. This study aimed to determine the effect of the addition of different attractants in pasta feed on the retention of protein, fat and energy of eel fish seed (Anguilla bicolor) stadia Elver. The experiment was conducted at the Faculty of Fisheries and Marine Resources, University of Airlangga. Proximate analysis of feed ingredients, fish proximate analysis of the beginning and end of the study conducted in Unit Inspection Service laboratory Consulting and Training Faculty of Veterinary Medicine, University of Airlangga. Research using a pasta feed plus attractants shrimp oil (A), eel oil (B), milkfish oil (C), squid oil (D) and without attractant (E) with the main parameters, namely retention of protein, fat and energy and support parameters that water quality, including temperature, solubility of oxygen, pH and ammonia. Analysis of data using Variety Analysis (ANOVA) and to determine the differences between treatments performed tests Distance Regression Duncan. The results showed that the addition of different attractant in feed pasta provide the best protein retention in treatment E (12.83%) and then a row followed by treatment C (11.98%), D (11.45%), B (10 , 26%) and A (7.78%). Best energy retention in treatment E (10.77%), then a row followed by treatment D (9.59%), C (9.47%), B (8.65%) and A (8.27% ). Best fat retention in treatment D (3.76%) and lowest in the C treatment (2.12%). Water quality maintenance media eel fish are temperature 28 º -28.5 º C, pH 7-8, dissolved oxygen 4-8 mg / L and ammonia from 1.5 to 2 mg / L.
Tingkat Kejadian Aeromonasis pada Ikan Koi (Cyprinus carpio carpio) yang Terinfeksi Myxobolus koi pada Derajat Infeksi yang Berbeda
[ Aeromonasis Incidence Rate In Koi (Cyprinus carpio carpio) That Infected Myxobolus koi At Different Degrees Of Infection ]
Authors: Mahasri Gunanti , Ulfiana Riris , Suprapto Hari
Published: February 2019

Abstract Myxobolus infection in koi fish allows followed by opportunistic microorganisms infection. Aeromonas hydrophila bacteria classified as opportunistic because it can cause illness in certain condition such as when environmental conditions change, stress and the condition of the host that has been infected by the parasite. This study purpose to determine the incidence rate Aeromonasis in koi (Cyprinus carpio carpio) infected Myxobolus koi at different degrees of infection. The results showed that of 120 samples taken, infected with M. koi in a mild degree of infection is 68.3%. Furthermore the medium infection is 22.5%, 1.7% severe infection and uninfected fish 7.5%. Each level of infection of M. koi taken samples for examination bacteria with skin samples and water. The results of bacterial examination showed that 17 positive samples of fish infected with A. hydrophila (85%) from 20 fish sample. 17 positive samples of fish infected with A. hydrophila consist of 3 sample normal fish, 7 sample M. koi mild infection, 6 sample M. koi medium infection and 1 sample severe infection. The results showed that the fish infected or not infected with M. koi, positive with A. hydrophila infection that needs to be done by using a bacterial colony calculation method of determining the Angka Lempeng Total (ALT) or Total Plate Count (TPC). The calculation results from the number of bacterial colony in various levels of infection of M. koi are sample 1 (normal koi fish) of 1.15 x108 CFU/ml, sample 2 (koi fish with a mild infection of M. koi) as much as 1.3 x108 CFU/ml. While the sample 3 (koi fish with a medium infection of M. koi) 2.02 x108 CFU/ml and sample 4 (with the severe infection of M. koi) 2.60 x108 CFU/ml.
Substitusi Bungkil Kedelai dengan Ddgs (Distillers Dried Grains and Solubles) terhadap Kandungan Lemak Kasar dan Energi Daging Ikan Nila Merah (Oreochromis niloticus)
[Substitution Of Soybean Meal With Ddgs (Distillers Dried Grains And Solubles) Content Of Crude Fat And Energy Meat Red Tilapia (Oreochromis niloticus) ]
Authors: Agustono Agustono , Inayah Sathiul , Al Arif M. Anam
Published: February 2019

Abstract Red tilapia (O. niloticus) is a food fish including efforts to increase the commodity into aquaculture production in 2014 amounted to 353%. To support efforts to increase production, so it needed one attempt that the cultivation of tilapia. One effort to reduce the cost of feed needed alternative feed ingredients were good quality. One of the alternatives that are cheap and high nutritional value is DDGS. DDGS is a byproduct from dry milling ethanol industry. Although the by-product of milling corn and the price is cheaper than soybean meal but the nutritional content is similar to soybean meal. From the results of the proximate analysis of DDGS contains 28% protein, 17% fat, 10% crude fiber, 27% and energy BETN 3150 kcal / kg. The results showed that the use of DDGS as a substitute for soybean meal until 80% did not cause significant differences (p> 0.05) on crude fat and energy content of meat red tilapia (O.niloticus).
Identifikasi dan Prevalensi Cacing pada Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) di Desa Ngrajek Magelang Jawa Tengah
[Identification And Prevalence Of Worms On Gouramy Gastrointestinal (Osphronemus gouramy) In Ngrajek Village Magelang Central Java]
Authors: Mahasri Gunanti , Aryani Riza , Kismiyati Kismiyati
Published: February 2019

Abstract Gouramy is one of the consumption of freshwater fish that have high economic value. Gouramy is a freshwater fish that have long been known and cultivated. Gouramy has many features including less demanding requirements for life, except that carp have slow growth. In maintenance appeared several constraints, such as illness. One possible cause is parasites. This research is aimed to know both the kind and the prevalence of gouramy gastrointestinal worms in Ngrajek Village Magelang Central Java. This research used survey methods through sampling at the site directly. Sample was taken once for 48 gouramy from four sites by using with fish size 10-15 cm. The main parameters of the research is the kind and prevalence degree of worms were found in the gastrointestinal of gouramy in the Ngrajek Magelang Central Java, while the supporting parameters in this research are water quality such as pH, temperature, dissolved oxygen (DO), and ammonia. The result of this research showed that from 48 gouramy from four sites, there was four samples that positively infected by gastrointestinal worms. The prevalence rate of gouramy infected with worms in the gastrointestinal for A location is 12.5%, for B location is 13.3%, for C location is 0%, and for D location is 10%. Worms prevalence rates are found in the gastrointestinal consists of Echinorhynchus 6.25%, Neoechinorhynchus 2.08% and Pallisentis 2.08%. Overall prevalence of gouramy infected with worms in the gastrointestinal in Ngrajek Village Magelang Central Java is 8.33%.
Pengaruh Konsentrasi Pupuk Lemna Minor terhadap Populasi Dunaliella salina
[Effect Of Lemna Minor Fertilizer Concentration On Dunaliella salina Population]
Authors: Rahardja Boedi S. , S Tjokorde Astrid , Masithah Endang Dwi
Published: February 2019

Abstract Dunaliella salina is a microalgae that have a high nutrient content, ctherefore Dunaliella salina widely used as an effective natural food, because they have cell walls and is easily digested. Dunaliella salina is rich in phytoplankton nutrients such as omega 3 and omega 6 and essential amino acids. Dunaliella salina has a large potential for the amount of β-carotene and glycerol. Lemna minor in addition containing nutrient n and p high also has a high concentration of minerals and pigment, especially beta carotene and xantofil so either for growth Dunaliella salina. This research aimed to know the effect of fertilizers on the growth of Lemna minor populations of Dunaliella salina and the optimal concentration of fertilizer Lemna minor in Dunaliella salina culture. Lemna minor doses used in this research are A (0 ml/l), B (0.25 ml/l), C (0.5 ml/l), D (0.75 ml/l), E (1 ml/l) and the use of fertilizers walne (1 ml/l) as with the control for the research. This research method is experimental methods. Research was done at the Faculty of Fisheries and Marine Laboratory, University of Airlangga for 7 days. The main parameter was observed the population Dunaliella salina. Supporting parameter includes water quality. The results suggest the best dose of fertilizer Lemna minor for the growth of Dunaliella salina is the dose in treatment D (0.75 ml/l) of 36.18x104 cells/ml, the highest growth occurred on the fifth day. Based on measurements of water quality throughout the treatment is still decent and good to support the the growth of Dunaliella salina during the research.
Pengaruh Lama Penyinaran dan Salinitas terhadap Pertumbuhan dan Jumlah Klorofil A Sargassum sp.
[Effect Of Photoperiod And Salinity On The Growth And Chlorophyll A From Sargassum sp. ]
Authors: Alamsjah Moch. Amin , Eismaputeri Maya Kartika , Rahardja Boedi Setya
Published: February 2019

Abstract Growth of Sargassum sp. in the waters of the world is different in each of its waters, it is strongly influenced by several factors, namely salinity and photoperiod. Salinity is closely related to the osmotic pressure that effects the body's balance of aquatic organisms. Photoperiod effects directly or indirectly, in particular on the algae, namely as a source of energy for photosynthesis. Photosynthesis process will occur not only with light, but also with the help of chlorophyll. The aim of this study was to determine the effect of photoperiod and salinity on growth and chlorophyll a Sargassum sp. and to determine the interaction relationship between photoperiod and of salinity on growth and chlorophyll a Sargassum sp. The design of the study is a Completely Randomized Design (CRD) Factorial test followed by Duncan's Multiple distance. Materials used in this study is Sargassum sp. with 9 treatments and 3 replications. Data results showed that the effect of salinity was not significantly different (F calculated < F table 0.05) on the growth of Sargassum sp. Effect of photoperiod did not different significant on the growth of Sargassum sp. in the first week until the third week, but different very significant (F calculated> F table 0.01) in the fourth week of treatment where E is the salinity of 30 ppt with photoperiod light 16 and 8 hours dark that significantly different with A, C, D, F, and I which not significantly different with B, G, H. On growth, there is no interaction of salinity and photoperiod. On chlorophyll a there is no difference significant in treatment A, B, C, D, E, F, G, H, and I. Salinity and photoperiod had no effect on chlorophyll a Sargassum sp., and there is no interaction of salinity and photoperiod.
Pemberian Enzim Lignosellulosa dalam Pakan Buatan Terhadap Pertumbuhan dan Tingkat Kelulushidupan Benih Ikan Gurami (Osphronemus gouramy Lac.)
[The Granting Of An Enzyme Lignosellulosa in Feed Artificial On The Growth and The Survival Rate Seed Gourami (Osphronemus gouramy Lac.) ]
Authors: Agustono Agustono , Akbar Muhammad Yusuf , Kusdarwati Rahayu
Published: February 2019

Abstract Gurami having economic values of. Have abundance, namely able in waters with its oxygen relatively low. Belong to the species of carnivorous herbs herbivora. Unprofitableness is eat feed derived from herbs with content nutrition relatively low compared to animals, so it impact on its growth slow, easly diseases, easly stress, hard eat and SR low. The purpose of this research isto know the addition of enzyme lignosellulase in artificialfeedincreased growth and increased survival rate seed gourami. Method research used is experimental with delightful random complete (RAL) with five treatment and four deuterenomy. The treatment used were : control (A), enzyme 550ml (B), enzyme 600ml (C), enzyme 650ml (D), and enzyme 750ml(E). The main parameters measured were growth rate, and survival rate. The supporting parameters observedwas water quality. Data analysis used analysis of variance (ANOVA) to know the effect of the treatments.To know the difference among treatments used Duncan’s Multiple Range Test (DMRT). The result showed that the provision of enzymes give a real power (p<0.01) against growth rate daily and growth long absolute gourami (Osphronemus gouramy) To survival rate and feed conversion ratio gourami give impact which is not dissimilar real. Growth best on treatment E (0.0214), then successive followed by treatment D (0.0174), C (0.015), B (0.0142) and A (0.0128). Conversion ratio feed on all treatment e 10,415, that is, e then successive followed by treatment d (12,915), c (14,3975), b (15,6375) and a (17,5325). Survival rate obtained 100%. Water quality media maintenance gourami is temperature 26ºC - 29ºC, pH 7 – 8, oxygen dissolved 3.5 – 5 mg/l and ammonia 0.004 – 0.005 mg/l.
Studi Bioakumulasi Logam Berat Merkuri (Hg) pada Teripang Lokal (Phyllophorus sp.) dari Pantai Timur Surabaya – Jawa Timur
[Study Of Bioaccumulation Heavy Metal Mercury (Hg) On Local Sea Cucumber (Phyllophorus sp.) from Surabaya East Coast – East Java]
Authors: Masithah Endang Dewi , Putri Rr. Febrina Anggraini , Arief Muhammad
Published: February 2019

Abstract Sea cucumber is a commodity fishery that is traded international. One of the sea cucumber that has economic value is the local sea cucumber (Phyllophorus sp.) believed that contain compounds can be immunomodulatory. Phyllophorus sp. is one of the three dominant species of sea cucumbers in abundance and distribution in the Surabaya East Coast (Winarni,dkk., 2010). Coastal environmental issues that deserve the attention is the problem of heavy metal pollution in coastal waters. The highest toxicity of heavy metals for aquatic animals and humans are mercury (Hg) (Widowati dkk, 2008). The purpose of this study was to determine levels of heavy metals bioaccumulation of mercury (Hg) in the local sea cucumber (Phyllophorus sp.) From the East Coast Surabaya and to determine the heavy metal content of mercury (Hg) in the local sea cucumber (Phyllophorus sp.) has exceeded the threshold or does not. The experiment was conducted on the East Coast Surabaya and analisys of heavy metal Mercury (Hg) in Balai Besar Laboratorium Kesehatan (BBLK) Surabaya. The method used is the method of field observation and analyzed descriptively. The results showed levels of mercury bioaccumulation of heavy metals (Hg) from BCFo-w value from 454.78– 802.05 and BCFo-s from 31,42 – 111,26. This shows Phyllophorus sp. have the ability to accumulate heavy metals with low until middle accumulation level category. Mercury content of the Phyllophorus sp. obtained range from 0.031 to 0.061 ppm, sea water ranged from 0.0015 to 0.0023 ppm and sediments ranged from 0.0417 to 0.112 ppm. Mercury levels in Phyllophorus sp. and sediment is below the NAB, but the sea water has exceeded from NAB. Water quality parameters are still in normal conditions in accordance with the quality standards KMLH (2004) that temperatures 26-28 º C, pH 7-8, salinity ranged from 28-29 ‰, DO value range 4-5 mg / L and brightness between 2-3 m.
Studi Kandungan Logam Berat Timbal (Pb) pada Ikan, Krustasea dan Moluska di Pantai Utara Bangkalan, Madura
[Study Of Heavy Metal Lead (Pb) In Fish, Crustaceans And Mollusks At The Northern Coast Of Bangkalan, Madura]
Authors: Arief M. , Nurhuda Reza , Rahardja Boedi Setya
Published: February 2019

Abstract Currently a variety of activities and industrial activity at Bangkalan increas the presence Suramadu bridge. It will provide an increasingly severe impact on the coastal environment (Purnomo, 2007). In 2010 it has been observed that the heavy metal content of lead at marine district Bangkalan average reached 0.083 mg/l. The value has exceeded the maximum threshold of Pb in water that can be used fishing activity is 0.008 mg/l so that the marine district. Bangkalan classified as polluted. The purpose of this study was to determine levels of heavy metal lead (Pb) in fish, crustaceans and mollusks in the northern Bangkalan beaches. This research activity covers observations in the field and analysis laboratory in April-May 2013. Water and sediment sampling conducted at three locations in the waters of the North Coast Klampis, Sepulu and Tanjung Bumi, while samples of fish, crustaceans and mollusks capture for fishermen in the three of subdistrict. The purpose of this study was to determine levels of heavy metal lead (Pb) in fish, crustaceans and mollusks in the northern coast Bangkalan. The research activities include field observations and laboratory analysis in April-May 2013. Water and sediment sampling conducted at three locations in the waters of the North Coast Bangkalan include Klampis beach, Sepulu beach and Tanjung Bumi beach,, while samples of fish, crustaceans and mollusks in the capture of three fishermen in the district later in the analysis of lead content using Atomic Absorption Spectrophotometry (AAS) in BBLK (Center for Health Laboratory) Surabaya. The results showed that the average content of heavy metals of lead on puffer fish 0.043 ppm, on manyung fish 0.095 ppm, on white shrimp 0.103 ppm, on crab 0.113 ppm, squid and mussels 0.149 ppm 0.08 ppm. The content has not exceeded the threshold value set by the government (fish: 0.3 ppm; crustaceans :0.5 ppm and mollusks : 1.5 ppm) (SNI, 2009) so it is still safe for human consumption. The average content of heavy metals in sea water is 0.135 ppm and sediment is 0.746 ppm. The content exceeds the threshold value set by the government (water: 0.008 ppm and sediment: 0.07 ppm) (SNI, 2009) so that in the long term is harmful to marine life that exist in the northern waters of the sea water quality Bangkalan.Water quality parameters in the coastal waters of the North Sea Bangkalan still in normal condition as habitat for marine life. Water quality parameters are still in good condition for growth in aquatic biota. Temperature ranges from 28 - 31oC, pH ranges from 6-8, dissolved oxygen ranged between 4-6 ppm, salinity ranged range 25-35 ppm.
Pengukuran Kecernaan Lemak Kasar, Bahan Organik dan Energi pada Pakan Ikan Nila (Oreochromis niloticus) dengan Menggunakan Teknik Pembedahan
[Measurements Of Extract Ether Digestibility, Organic Matter and Energy Feed In Tilapia (Oreochromis niloticus) Using Surgery Techniques]
Authors: Agustono Agustono , Wicaksono Rahardian , Lokapirnasari Widya Paramita
Published: February 2019

Abstract Tilapia is a freshwater fish species are widely cultivated in Indonesia. Tilapia production continues to grow every year, the average increase in the number of experienced production tilapia reaches 22.24% in the 2010-2011 range. Tilapia production in 2011 reached 567.449 tons and was second aquaculture production by major commodity after seaweed. This study aimed to determining differences in extract ether digestibility, organic matter, and energy on feed derived from different factories in tilapia (Oreochromis niloticus) using surgery techniques. The research design is using completely randomized design. The variables were measured extract ether digestibility, organic matter, and energy in tilapia feed. Analysis of the data processed using Analysis of Variance (ANOVA) to determine differences in treatment given. If there is a difference then followed by Duncan's Multiple Range Test with a significance level of 5%. Based on the findings that there were no significant differences (p>0.05) in extract ether , organic matter, and energy digestibility of the feed value of tilapia. The results stated that the feed treatments A, B and C efficiently digested by tilapia.
Uji Kadar Albumin dan Pertumbuhan Ikan Gabus (Channa striata) dengan Kadar Protein Pakan Komersial yang Berbeda [Albumin Level Test and Snakehead Fish (Channa striata) Growth with Different Commercial Feed Protein Level]
Authors: Alamsjah Moch. Amin , Kusumaningrum Galuh Ajeng , Masithah Endang Dwi
Published: February 2019

Abstract Gabus fish is rich of albumin. This fish as albumin source for hypoalbumin (lower albumin) patients and wound, both post operation wound and burn. Albumin have many sulfhydrile compound (SH) have a function as radikal bind. Protein which have rich of –SH compound be able to binding hazard metal and also antioxidant effects and salic acid. At present, albumin produced from human blood, then the price is expensive enough. The finding of albumin extract from gabus fish then become alternative to get cheap albumin. This research purposed to recognize about the influence by providing different protein feed on albumin level and the growth of gabus fish. Method that used in this research is experimental method, with Complete Random Design as trial design. Treatment that given such different protein level included, Po treatment with 26% protein, P1 treatment with 29% protein, P2 treatment with 32% protein respective repeated six times. The main parameter that observed are albumin and growth of Gabus fish. Supporting parameter that observed in this research are temperature measurement, pH and DO, Variant Analysis (ANOVA) and to know about Duncan Multiple Range test treatment. The result of the research indicates that found different albumin level on Gabus fish is not real among treatment above on observation yields. The highest average of albumin level found in P2 treatment, while lowest influence taken from treatments of P0 and P1. The growth of Gabus fish with providing commercial feed with different protein level indicates that found different growth of real Gabus fish (p< 0,05). The highest yield taken from P2 treatment is really different with other treatments. While lowest yield taken from P0 and P2 treatment.
Bioaktivitas Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Terhadap Respon Fisiologis (Glukosa Darah Dan Tachiventilasi) Ikan Koi (Cyprinus carpio) [Bioactivity Essential Oil Of Bandotan Leaf (Ageratum conyzoides) On Koi (Cyprinus carpio) Physiological Responses (Blood Glucose And Tachiventilation) ]
Authors: Sulmartiwi Laksmi , Umami Mardiah Rahma , Alamsjah Moch. Amin , Subekti Sri
Published: February 2019

Abstract Koi (C. carpio) is one of famous ornamental fish in Indonesia. Fish often be subject of change or stessor like physical, chemical and biology on culture. Stress in fish caused released cortisol hormone and cathecolamine hormone. Cathecolamine increase glicogenolisis, cardiovascular regulation and respiration fungtion. Blood glucose and tachiventilation (opercular movement) can indicate stress respon in fish. Essential oil of A. conyzoides have central analgesic activity. Analgesic drugs in vertebrae can reduce stress in fish. Essential oil shown inhibitory effect of GABA transminase, an enzime GABA (gammaaminobutryic acid) degradation which can provoking sedation. Sedation effect in fish reduce blood glucose and tachiventilation. Potency bioactive essential oil of bandotan leaf compound haven’t been know in aquaculture. The potency based of LC50 (Lethal Consentration 50 %) value Purpose of this research determined effect of essential oil Ageratum conyzoides leaf on Koi (Cyprinus carpio) Phsycology Response (Blood Glucose and Tachiventilation) every 4 hours durung 24 hours and LC50-24 hours. Research was conducted at Laboratory of Faculty of Fisheries and Marine Airlangga University Surabaya. Research design used Completely Randomized Design and followed by Duncan's Multiple range test. Results of analysis of variance (ANAVA) from early observation and every 4 hours during 24 hours day showed that each treatment of essential oil Ageratum conyzoides leaf gave a significantly different effect on the blood glucose and tachiventilation (P <0.05). The highest average of blood glucose was found in D treatment at second observation (443,67 mg/dl) and the lowest was found in C treatment (60,67 mg/dl). The highest average of tachiventilation was found in D treatment at first observation (1730 bit/9 minute) and the lowest was found in A treatment (437,67 bit/9 minute) at early observation. LC50-24 hours essenial oil Ageratum conyzoides leaf is 34,047 ppm. Based on measurements of water quality, treatments was still viable and good to supported viability of Cyprinus carpio during research, water temperature range between 29-31oC, range of dissolved oxigen 4-6 mg/l, pH ranges 7,5-8,3 and 0,0060,27 mg/l on ammonia.
Identifikasi Koi Herpesvirus pada Dosis yang Berbeda dengan Metode Imunohistokimia Streptavidin Biotin pada Ikan Nila (Oreochromis niloticus)
[Identification of Koi Herpes Virus At Different Dose with Streptavidin Biotin Methods Immunohistochemistry on Tilapia (Oreochromis niloticus)]
Authors: Suprapto Hari , Nurani Faricha Risma , Suwarno Suwarno
Published: February 2019

Abstract KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations. Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus). This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000. The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms
Keragaman Gen Cytochrome B pada Sidat (Anguila bicolor) Berdasarkan Restriction Fragment Length Polymorphism (RFLP)
[Genetic Diversity Cythochrome B of Sidat (Anguila bicolor) Assesed by Restriction Fragment Length Polymorhisme (RFLP) ]
Authors: Mahasri Gunanti , Wilujeng Lestari , Mufasirin Mufasirin
Published: February 2019

Abstract This study aims to analyze the genetic character of Anguilla bicolor based on cytochrome b gene as the basis of information in the study of phylogeny and genetic engineering. The research was conducted from May to September 2013 in the Laboratory of Biotechnology Faculty of Science, University of Brawijaya. This study uses a survey with qualitative descriptive analysis in the laboratory. Samples obtained from direct arrests in Tulungagung Popo Beach , Manado , Medan and Cilacap. Study was initiated by DNA isolation using CTAB method and followed by PCR . Primers used were cytb - 1 (5' - TGCTAACGATGCCCTAGTGG - 3 ') and b CYT - 2 (5' - CTAGTCAACCTACT - AATGGG - 3 ') . PCR results were cut using restriction enzymes and Msp1 Hha1. Data analysis was performed with the aid of NTSYS software program. Genetic character of a sequence of nucleotide bases making up DNA from the cytochrome b gene were obtained on each sample has a degree of similarity around 32 - 100 %.
Monitoring Kualitas Air pada Tambak Pembesaran Udang Vannamei (Litopenaeus vannamei) di Situbondo, Jawa Timur
[Monitoring of Water Quality on Rearing Ponds of Vannamei Shrimp (Litopenaeus vannamei) in Situbondo, Jawa Timur ]
Authors: Manan Abdul , Putra Fatchurizal Rama
Published: February 2019

Abstract Vannamei shrimp (Litopenaeus vannamei) is one of fishery commodities that have high economic value in international market, so the vannamei shrimp farming can be reliable. Success in maintaining of water quality is the key factor in the cultivation. Either directly or indirectly, the water quality has important role in determining the growth and survival of vannamei shrimp. Water quality parameters that contribute to survival in vannamei shrimp growth include water temperature, dissolved oxygen, water pH, salinity and ammonia. The purposed of this study is to know the conditions of water quality on enlargement ponds of vannamei shrimp (Litopenaeus Vannamei) in Situbondo. Study was held on January 16 to February 16, 2012. The methods used in this study is descriptive method. Water management activities on enlargement vannamei shrimp includes good water quality observed in biological, physical, and chemical. In addition to the observation of water quality, maintenance, enlargement of vannamei shrimp also save good preparation includes construction of pond and container preparation and feeding. Daily observation data acquisition carried out water sampling done one time and early morning. Sampling using bottle samples. Get the data covering the temperature range between 27-30 ºC, salinity between 25-28 ppt, pH ranged from 7.0 to 9.0, DO levels ranged from 4.0 -4.8 mg / L, ≤ 0.001 ppm levels of ammonia, BOD from 1.3 to 1.75 ppm, plankton here tend to be kind Diatomae, Chlorella sp, Copepods, Rotifera, Ocylatoria.
Analisis Perbedaan Nilai Konsentrasi Logam Berat Cadmium (CD) pada Rumput Laut (Eucheuma cottonii) di Perairan Pamekasan Dan Sumenep – Madura
[Analysis of Heavy Metal Concentration Difference Value on Cadmium (CD) Against Seaweed (Eucheuma cottonii) in Pamekasan and Sumenep Seashore - Madura]
Authors: Arief Muhammad , Setiabudi Denny , Rahardja Boedi Setya
Published: February 2019

Abstract Indonesia is well known as an island nation whose territory is ocean and coastline. One of cultivation are known to have high economic value is seaweed (Eucheuma cottonii). Madura sea is one of the sea that the majority of communities dependent on the seaweed by means of cultivated seaweed in the Madura sea, for the example is E.cottonii seaweed. Seaweed is also useful as a tool to detect the presence of heavy metal cadmium in other organisms, such as scallops, shrimp and fish. The purpose of this research was to determine the difference between the levels of cadmium that accumulates in seaweed E.cottonii on Pamekasan and Sumenep seashore and compared the levels of cadmium in seaweed E.cottonii the provisions of the Indonesian National Standard. Tests performed in the Great Hall of sampling Health Laboratory (BBLK) Surabaya. This research was conducted in Jumiang – Pamekasan seashore, and the Saronggi – Sumenep seashore. The materials used for sampling in this study is E.cottonii wet seaweed with 38 days of age weighing 4.53721 kg at each station in Sumenep and 3.47243 kg at each station in Pamekasan. The main parameters were observed in this research is the sum value of accumulated heavy metal cadmium in seaweed. Auxiliary parameters such as water quality and sediment values in seaweed. On the results of research on the analysis of the difference in value against the concentration of heavy metal cadmium in seaweed E.cottonii on Pamekasan seashore can still be said does not exceed a threshold value that is equal to 0.0182 ppm and 0.0262 ppm. However, The value of the levels of heavy metals in waters declared Sumenep exceeds a threshold value that is equal to 0.1314 and 0.1298.
Kejadian Kelainan Vertebra Ikan Nila (Oreochromis niloticus) pada Media Penetasan Salinitas yang Berbeda
[Vertebrae Malformation Tilapia Fish (Oreochromis niloticus) on Different Media Hatching Saline]
Authors: Lutfiyah Lailatul , Triastuti Rr Juni , Masithah Endang Dewi , Darmanto Win
Published: February 2019

Abstract Tilapia fish belonged to the euryhaline fish, which has wide range tolerance in salinity (freshwater euryhaline fish has salinity tolerance >8 ppt). Incidence of fish skeletal abnormalities could be the response of the environment influence such as salinity, temperature and hypoxia. This research uses five treatments : 0,5 10, 15, 20 ppt. The results showed that the highest abnormalities found on the treatment of 15 ppt and 20 ppt, while the lowest for the treatment of abnormalities of 10 ppt. The best result to be used in the brackish nila aquaculture is 10-15 ppt in salinity.
Deteksi Koi Herpesvirus (KHV) pada Ikan Nila (Oreochromis niloticus) yang Diinfeksi secara Buatan [ Detection of Koi Herpesvirus (KHV) in Nile Tilapia (Oreochromis niloticus) Infected by Artificially Infection ]
Authors: Suprapto Hari , Suwarno Suwarno , Pradana Muhammad Sungging
Published: February 2019

Abstract Koi Herpesvirus (KHV) was formerly known as Cyprinid herpesvirus-3 (CyHV-3) and carp nephritis interstitial and necrosis gill virus (CNGV) is a virus that infects common carp and koi (Cyprinus carpio and C. carpio koi) in farmed and wild population. KHV cause disease at a temperature of 18-25 °C with mortality rate of 80-90 % in fry and adult fish. Currently KHV also detected in tilapia from the results of monitoring in the field. The presence of KHV in tilapia can occur as a result of maintenance in cages adjacent to the infected carp. KHV diagnostic method currently based on case definition and PCR (Polymerase Chain Reaction) detection. The basic concept of PCR is one DNA molecule is used to produce two copies, then four, then eight and so forth through multiplication by polymerase. PCR results sometimes indicated the presence of a faint band caused a low amount of virus, so it is necessary to investigate the presence of KHV DNA in tilapia using different doses of infection. This study aimed to determine the KHV infectivity in nile tilapia were infected by artificially infection and determine dose KHV infection that can infect nile tilapia. The study design used true experimental with with the presentation of descriptive data. Dose of viral infection are 1 ID50, 10 ID50, 100 ID50 and 1000 ID50. The results showed that no clinical symptoms of KHV infected in nile tilapia. The results of electrophoresis of PCR products showed that the mucus of nile tilapia were infected with a 1000 ID50 immersion dose showed thin bands. The same results are also shown in the gill of nile tilapia infected by gill spray at 1000 ID50 dose. Fish were infected by injection, KHV was not detected in mucus, gill, kidney and liver. The results above show nile tilapia cannot be infected by KHV on various treatment
Analisis Respons Imun Ikan Koi (Cyprinus carpio Koi) yang Divaksin dengan Whole Protein Spora Myxobolus koi sebagai Kandidat Vaksin Myxobolusis [ Immune Response Analysis of Fish Koi (Cyprinus carpio Koi) Vaccinated Myxobolus Koi Spores Whole Protein as Vaccine Candidate Myxobolusis]
Authors: Mahasri Gunanti , Yusuf Mohamad , Mufasirin Mufasirin
Published: February 2019

Abstract Myxobolus is one of parasites on koi fish that belongs to a class myxosporea that can infect and systemic and can cause harm to the fish farming. Vaccination is an attempt to cause-specific endurance through vaccination. Observations differential leukocytes and increased optical density values can be used to determine the effectiveness of the vaccine is given. This study aims to analyze the immune response koi fish vaccinated with Myxobolus koi spores whole protein for vaccine development myxobolusis in koi fish. The method used in this study is Completely Randomized Design with 4 treatments and 5 replications. The results showed that a change in the total number and types of leukocytes that can be used as indicators of the presence of certain infectious diseases that occure in fish. The highest value of lymphocytes in treatment B, monocytes highest in treatment D, neutrophils on treatment D, eosinophils on treatment A and basophils highest in treatment A. The observation of the highest optical density value in treatment B (fish vaccinated and infected 80 M. koi spores / tail) of 0.593 at day 30, while the lowest in treatment D (fish are not vaccinated but diinveksi 80 M. koi spores / tail) of 0,064 in 30 days