bluuebunny/crossref_metadata_2025 huggingface dataset:
The College Students’ Oral English Education Strategy Using Human-Computer Interaction Simulation System From the Perspective of Educational Psychology
Authors: Zhou Ping , Wu Xiaoliang , Xu Hui , Wang Guan
Published: October 2021

The role of the human–computer interaction (HCI) system in college students’ oral English learning is discussed to analyze the current situation of college students’ oral English based on the HCI simulation system. The purpose is to study the oral education of college students. First, the theories of educational psychology, the HCI system, and the current situation of college students’ oral English learning are elaborated. Meanwhile, in oral English teaching, teachers use support vector machines and multimodal fusion intention perception methods in set theory to realize the interactive teaching between students and machines; then, the HCI simulation of oral English is explained. The current situation of college students’ oral English learning is analyzed by a questionnaire from the perspective of educational psychology. Finally, the HCI system in college students’ oral English learning is explored based on the learning level detection. The results show that 12% of college students are unqualified in oral English; 25% of them think their oral English level is medium; most of college students’ English learning anxiety is related to English progress anxiety; 18% of the students believe that they will study oral English for life; 32% of the students think that they have more opportunities to learn English at ordinary times; and most of the students learn English through English movies and songs outside of class. What attracts college students to learn oral English through the HCI system is that learning is not limited by time and space. Most students believe that their English level is good and hope that learning anxiety can be reduced through HCI systems. The strategies of college students’ oral English education with an HCI simulation system are evaluated based on the perspective of educational psychology, providing a research basis for oral English education in other regions and even the whole country to facilitate the better development of oral English education.
Identification and Characterization of Circulating MicroRNAs as Novel Biomarkers in Dogs With Heart Diseases
Authors: Ro Woong-Bin , Kang Min-Hee , Song Doo-Won , Kim Heyong-Seok , Lee Ga-Won , Park Hee-Myung
Published: October 2021

Background: Previous studies in humans have confirmed dysregulations of circulating microRNAs (miRNAs) in patients with various cardiovascular diseases. However, studies on circulating miRNAs in dogs with various heart diseases are limited in number. This study aimed to identify significantly dysregulated circulating miRNAs and characterize them as novel biomarkers in dogs with heart diseases.

Materials and Methods: Circulating levels of 11 miRNAs were investigated in serum samples of 82 dogs (72 with heart diseases and 10 healthy dogs) using quantitative reverse transcription-polymerase chain reaction. The results were correlated to clinical data including echocardiographic results and N-terminal pro B-type natriuretic peptide (NT-proBNP) levels.

Results: Upregulation of cfa-miR-130b was observed in dogs with myxomatous mitral valve degeneration (MMVD) stage B, patent ductus arteriosus, and pulmonic stenosis. In dogs with MMVD stage B, cfa-miR-130b was upregulated and correlated with clinical indices. In receiver operating characteristic (ROC) analysis, cfa-miR-130b accurately distinguished dogs with diseases from healthy dogs. We also observed that cfa-miR-375 and cfa-let-7b were upregulated in dogs with concentric cardiac hypertrophy. The cfa-miR-375 was correlated with concentric hypertrophy indices and was an accurate indicator of concentric hypertrophy in ROC analysis.

Conclusions: The miRNAs identified in this study may be used as novel biomarkers and possible candidates for therapeutic targets in various canine heart diseases.
A Unified Formulation of Analytical and Numerical Methods for Solving Linear Fredholm Integral Equations
Authors: Providas Efthimios
Published: October 2021

This article is concerned with the construction of approximate analytic solutions to linear Fredholm integral equations of the second kind with general continuous kernels. A unified treatment of some classes of analytical and numerical classical methods, such as the Direct Computational Method (DCM), the Degenerate Kernel Methods (DKM), the Quadrature Methods (QM) and the Projection Methods (PM), is proposed. The problem is formulated as an abstract equation in a Banach space and a solution formula is derived. Then, several approximating schemes are discussed. In all cases, the method yields an explicit, albeit approximate, solution. Several examples are solved to illustrate the performance of the technique.
Identity Reinforcement or Risky Organizational Change? Category Spanning in Humanitarian Projects
Authors: Kooijman Eva A. P. , Beck Nikolaus
Published: October 2021

In this study, we investigate the consequences of organizational change that consist of adding new categories to the portfolio of humanitarian organizations. Our aim is to discern differences in these consequences between specialist and generalist organizations. Previous research has shown that spanning categories lead to disadvantages in the evaluation of organizations by audience members in terms of the attention they receive from the audience but did not focus on the distinction between specialists, organizations that have no history in spanning categories, and generalists, organizations that have already done so in the past. Using fixed effect logit regression methods on project approval among 2480 non-governmental organizations (NGOs) in the humanitarian sector, we show that category spanning is tantamount to risky organizational change for specialist organizations, which leads to a reduction in project approval. However, generalists benefit from category spanning, which indicates identity reinforcement. We also show that in the case of urgent demand, spanning categories has a less detrimental effect. Consequently, organizations that have successfully undergone a change from a specialist to a generalist identity no longer suffer from category spanning. Moreover, also situations of urgent demand reduce the negative consequences of category spanning.
Potential Role of Technology Innovation in Transformation of Sustainable Food Systems: A Review
Authors: Khan Nawab , Ray Ram L. , Kassem Hazem S. , Hussain Sajjad , Zhang Shemei , Khayyam Muhammad , Ihtisham Muhammad , Asongu Simplice A.
Published: October 2021

Advanced technologies and innovation are essential for promoting sustainable food systems (SFSs) because these technologies can be used to answer some of the critical questions needed to transform SFSs and help us better understand global food security and nutrition. The main objective of this study is to address the question of whether technological innovations have an impact on the transformation of SFSs. There are certain innovations including agricultural land utilization, food processing, production systems, improvement in diets according to people’s needs, and management of waste products. This study provides an overview of new technologies and innovations being used with potential to transform SFSs. Applications of emerging technologies in digital agriculture, including the Internet of Things (IoT), artificial intelligence and machine learning, drones, use of new physical systems (e.g., advanced robotics, autonomous vehicles, advanced materials), and gene technology (e.g., biofortified crops, genome-wide selection, genome editing), are discussed in this study. Additionally, we suggest eight action initiatives, which are transforming mindsets, enabling social licensing, changing policies and regulations, designing market incentives, safeguarding against undesirable effects, ensuring stable finance, building trust, and developing transition pathways that can hasten the transition to more SFSs. We conclude that appropriate incentives, regulations, and social permits play a critical role in enhancing the adoption of modern technologies to promote SFSs.
Analysis on Transporting Methods of Cultivation Unit for Vertical Cultivation in Plant Factory
Authors: Gu Song , Ji Hanhan , Yang Yanli , Chu Qi , Yang Yi , Liu Houcheng , Jiang Xianping
Published: October 2021

Automatic transport can improve the operational efficiency in plant factory production and reduce the use of labor. However, a determination of a plant factory automation operation mode should comprehensively consider the economic strength of the enterprise, operation objects and operation mode, production scale, technical strength, labor costs, and other factors. In this study, a logistics transport system comprising a set of cultivation units was developed for plant factories, using a mode based on shelf-end delivery without power inside the shelf. Moreover, an analysis was conducted on four modes of common transportation methods for the cultivation units for vertical cultivation in plant factories. The results showed that, when comparing the progressive transport type and warehousing/reciprocating transport type for cultivation units, the transport productivity of the former is higher than that of the latter, and the difference in the two transport productivities is proportional to the capacity for cultivation units in each layer. When the capacities for cultivation units in each layer are 20 and 40, the transport productivity of the former is 115–200% and 130–250% higher than that of the latter, respectively. Moreover, the logistics transport system developed herein reaches an input (output) transport productivity of 330 (270) cultivation units h−1.
Modelling Broiler Requirements for Lysine and Arginine
Authors: Nogueira Bernardo Rocha Franco , Sakomura Nilva Kazue , Reis Matheus de Paula , Leme Bruno Balbino , Létourneau-Montminy Marie-Pierre , Viana Gabriel da Silva
Published: October 2021

Six assays were conducted to investigate male and female broiler responses to standardized ileal digestible (SID) lysine (Lys) and arginine (Arg). Response data were modeled to estimate the efficiency of utilization (k) of both amino acids and adjust factorial models to predict bird intake for SID Lys and SID Arg. In each assay, 1280 Cobb 500® broilers (640 male and 640 female) were randomly assigned to one of sixteen dietary treatments with four replicates of 20 birds. Dietary treatments consisted of crescent levels of SID Lys or SID Arg based on the dilution technique. The values of k determined for each phase (1- to 14-, 15- to 28-, and 29- to 42-d-old) and sex were contrasted using linear regression with groups (sex and phase). The estimated efficiencies were 0.79 for Lys and 0.62 for Arg, which were unaffected by phase or sex. Factorial models based on body weight and weight gain (M1) and on body and feather protein weight and deposition (M2) were applied to estimate the SID Lys and Arg intake for growth. The amino acid intake based on M2 had a lower error of prediction. Broiler chickens require 94.9 and 92.9 mg/d of SID Lys and SID Arg to deposit 1 g of body protein.
Determinants of Harem Size in a Polygynous Primate: Reproductive Success and Social Benefits
Authors: Xia Wancai , Grueter Cyril C. , Ren Baoping , Zhang Dejun , Yuan Xiaoxia , Li Dayong
Published: October 2021

We used long-term data on the variation in harem size in Yunnan snub-nosed monkeys to research the effects of harem size on reproductive success and the ratio of grooming received to given (RGRG). The results suggest that harem holders derive reproductive benefits commensurate with harem size, whereas the females’ reproductive success is unaffected by harem size. Males of larger harems groomed less and had higher RGRG than males of smaller harems. In the case of females, grooming given increased, and RGRG decreased with an increase in harem size. The males’ reproductive success seems to be a driver of harem size maximization. From the females’ perspective, dwindling social benefits appear to set the upper limit for harem enlargement. We also showed that males of monogamous units (‘single-female harems’) invested more into grooming their female, presumably to prevent unit disintegration and loss of mating privileges.
Sedation and Anesthesia of Galapagos (Chelonoidis nigra), Aldabra (Aldabrachelys gigantea), and African Spurred Tortoises (Centrochelys sulcata): A Retrospective Review (2009–2019)
Authors: Turner Rachel C. , Gatson Bonnie J. , Hernandez Jorge A. , Alexander Amy B. , Aitken-Palmer Copper , Vigani Alessio , Heard Darryl J.
Published: October 2021

Tortoises belong to the taxonomic family Testudinidae, which is considered one of the most imperiled families of the order Testudines. Anesthesia is often required for the medical and surgical management of large tortoises. The objectives of this retrospective study were to review drug regimens used to successfully anesthetize Galapagos (Chelonoidis nigra), Aldabra (Aldabrachelys gigantea) and African spurred (Centrochelys sulcata) tortoises, and to compare the times to effect and to extubation in tortoises administered different premedication protocols. Anesthetic records of giant tortoises admitted to the University of Florida College of Veterinary Medicine between January 2009 and December 2019 were reviewed. A total of 34 tortoises (six Aldabra, 23 Galapagos, and five African spurred) were included, resulting in 64 anesthetic events. Frequently used premedication protocols included an α2-adrenergic agonist and ketamine combined with either midazolam (group α2−adrenergic agonist, midazolam, ketamine, AMK; n = 34), a μ-opioid receptor agonist (group α2−adrenergic agonist, μ-opioid receptor agonist, ketamine, AOK; n = 13), or a μ−opioid receptor agonist and midazolam (group α2−adrenergic agonist, midazolam, μ-opioid receptor agonist, ketamine, AMOK; n = 10). Inhalant anesthetics (isoflurane, n = 21; sevoflurane, n = 23) were frequently used for maintenance of anesthesia following premedication. Out of the 34 total tortoises, 22 had only one anesthetic event, five had two anesthetic events, three had three anesthetic events, and four had four or more anesthetic events. Few adverse effects were observed and there was no mortality reported during the peri-anesthetic period. Sedation and general anesthesia of giant tortoises can be successfully performed with a combination of an α2-adrenergic agonist and ketamine in combination with midazolam and/or a μ−opioid receptor agonist.
Survival of Embryos and Fry of Sea Trout (Salmo trutta m. trutta) Growing from Eggs Exposed to Different Concentrations of Selenium during Egg Swelling
Authors: Dziewulska Katarzyna , Kirczuk Lucyna , Czerniawski Robert , Kowalska-Góralska Monika
Published: October 2021

Adequate selenium (Se) availability enhances the health and growth of organisms, but overdose of it can be harmful and pathogenic. The study’s objective was to analyse the impact of short-term exposure of sea trout fertilised eggs to inorganic selenium (SeO2) at concentrations from 0 to 32 mg Se L−1 to find the optimal and toxic dose of Se on early fish development. Se accumulated in the body, embryos’ survival rate, and growth in the first four months of life was examined. Swelling of fertilised eggs in water supplemented with Se at a concentration from 0.5 to 8 mg Se L−1 was associated with a slightly positive impact on the hatching rate. At higher Se concentration, a harmful effect on the survival of the embryo was observed. The survival of fry was similar in all groups, while the fry length and weight correlated positively with Se concentration in its body. Immersion of fertilised eggs in water enriched with Se during egg swelling can constitute a method to supplement the element to non-feeding stages of fish. In selenium-poor areas, this innovative method can be implemented in aquaculture to improve breeding outcomes. Se concentration should be adjusted to the chemical compound, fish species, and Se’s content in the yolk.
Serum Level of Tumor-Overexpressed AGR2 Is Significantly Associated with Unfavorable Prognosis of Canine Malignant Mammary Tumors
Authors: Yuan Stephen Hsien-Chi , Chang Shih-Chieh , Huang Yenlin , Liu Hao-Ping
Published: October 2021

Canine malignant mammary tumors (MMTs) are prevalent malignancy in intact female dogs with a high incidence of metastasis and recurrence. A current lack of easily accessible tumor biomarkers hinders a timely assessment of the disease outcome. We previously identified anterior gradient protein 2 (AGR2) with higher protein abundance in canine MMT tissues compared with normal counterparts. AGR2 is an endoplasmic reticulum-resident protein disulfide isomerase involved in the regulation of protein processing and also exists extracellularly via secretion to exert pro-oncogenic functions. In the present study, we validated overexpression of AGR2 in canine MMT tissues from 45 dogs using immunohistochemistry and immunoblotting, and assessed serum AGR2 levels in 81 dogs with MMTs and 21 benign cases using a competitive enzyme-linked immunosorbent assay (ELISA). Our data revealed that serum eAGR2 levels are significantly correlated with MMT progression (p = 0.0007) and remote tumor metastasis (p = 0.002). Moreover, elevated levels of serum eAGR2 are associated with an unfavorable overall survival of MMT dogs in later stage (p = 0.0158). Area under the time-dependent ROC curve (AUC) of serum eAGR2 level as a prognostic indicator was 0.839. Collectively, this study uncovered that serum eAGR2 level is significantly associated with an adverse outcome of MMT dogs and holds a predictive potential in MMT prognosis.
Genomic Regions Associated with the Position and Number of Hair Whorls in Horses
Authors: Lima Diogo Felipe Pereira de Assis , da Cruz Valdecy Aparecida Rocha , Pereira Guilherme Luís , Curi Rogério Abdallah , Costa Raphael Bermal , de Camargo Gregório Miguel Ferreira
Published: October 2021

The position and number of hair whorls have been associated with the behavior, temperament, and laterality of horses. The easy observation of whorls assists in the prediction of reactivity, and thus permits the development of better measures of handling, training, mounting, and riding horses. However, little is known about the genetics involved in the formation of hair whorls. Therefore, the aim of this study was to perform a genome-wide association analysis to identify chromosome regions and candidate genes associated with hair whorl traits. Data from 342 Quarter Horses genotyped for approximately 53,000 SNPs were used in an association study using a single-step procedure. The following traits were analyzed: vertical position of hair whorl on the head, number of whorls on the head, and number of whorls on the left and right sides of the neck. The traits had between one and three genomic windows associated. Each of them explained at least 4% of the additive variance. The windows accounted for 20–80% of additive variance for each trait analyzed. Many of the prospected genes are related to hair follicle growth. Some of these genes exert a pleiotropic effect on neurological and behavioral traits. This is the first indication of biological and physiological activity that might explain the association of hair whorls and temperament.
Ecological Analysis of the Helminth Community of the Wood Mouse, Apodemus sylvaticus, along an 18-Year Post-Fire Regeneration Period in a Mediterranean Ecosystem
Authors: Sáez-Durán Sandra , Debenedetti Ángela L. , Sainz-Elipe Sandra , Sabater-Tena Mireia , Galán-Puchades María Teresa , Fuentes Màrius Vicent
Published: October 2021

The role of helminths of the wood mouse, Apodemus sylvaticus, as biological indicators of the post-fire regeneration process in Serra Calderona Natural Park, a Mediterranean forest ecosystem located between the provinces of València and Castelló (Valencian Country, Spain), has been analysed for almost twenty years. The helminth ecological analysis of 917 A. sylvaticus (675 originating from the burned area and 242 originating from the control area) has been carried out between the 2nd and 18th post-fire years. The influence of intrinsic (host population density, sex and age) and extrinsic (site, period and year of capture, climate variables) factors on the post-fire evolution of the helminth community of the wood mouse, and the biodiversity, species richness and life cycle of the helminth species was studied. Taking into account the most important results obtained, various aspects of the helminth community dynamics of the wood mouse are confirmed as biological indicators of the post-fire regeneration process in Mediterranean ecosystems. The still existing differences between the two areas are mainly related to the influence of climate variables on the post-fire regeneration process. Moreover, the important role that helminth parasites of the wood mouse play as biological indicators of this process in Mediterranean ecosystems is demonstrated.
Evaluation of the Respiratory Microbiome and the Use of Tracheal Lavage as a Diagnostic Tool in Kemp’s Ridley Sea Turtles (Lepidochelys kempii)
Authors: McNally Kerry L. , Bowen Jennifer L. , Brisson Jennifer O. , Kennedy Adam , Innis Charles J.
Published: October 2021

Respiratory disease is a common cause of morbidity and mortality in sea turtles, including the Kemp’s ridley sea turtle (Lepidochelys kempii). Although culture-dependent methods are typically used to characterize microbes associated with pneumonia and to determine treatment, culture-independent methods can provide a deeper understanding of the respiratory microbial communities and lead to a more accurate diagnosis. In this study, we characterized the tracheal lavage microbiome from cold-stunned Kemp’s ridley sea turtles at three time points during rehabilitation (intake, rehabilitation, and convalescence) by analyzing the 16S rRNA gene collected from tracheal lavage samples. We retrospectively developed a radiographic scoring system to grade the severity of lung abnormalities in these turtles and found no differences in diversity or composition of microbial communities based on radiographic score. We also found that the culture isolates from tracheal lavage samples, as well as other previously reported sea turtle pathogens, were present in variable abundance across sequenced samples. In addition to the tracheal microbial community of live turtles, we characterized microbial communities from other segments of the respiratory tract (glottis, trachea, anterior lung, posterior lung) from deceased turtles. We found a high degree of variability within turtles and a high degree of dissimilarity between different segments of the respiratory tract and the tracheal lavage collected from the same turtle. In summary, we found that the pulmonary microbial community associated with pneumonia in sea turtles is complex and does not correlate well with the microbial community as identified by tracheal lavage. These results underscore the limitations of using tracheal lavage for identification of the causative agents of pneumonia in sea turtles.
Effect of Total Starch and Resistant Starch in Commercial Extruded Dog Foods on Gastric Emptying in Siberian Huskies
Authors: Richards Taylor L. , Rankovic Alexandra , Cant John P. , Shoveller Anna K. , Adolphe Jennifer L. , Ramdath Dan , Verbrugghe Adronie
Published: October 2021

Gastric emptying rate (GER) may impact diabetes and obesity in humans and could provide a method to reduce canine weight gain. Starch, the most common source of carbohydrates (CHOs) in pet food, is classified as rapidly or slowly digestible, or resistant to digestion. This study investigated starch source effects in commercial extruded dog foods on the GER of 11 healthy adult Siberian Huskies. Test diets were classified as traditional, grain-free, whole-grain, and vegan. Dogs received each diet once, a glucose control twice, and acetaminophen (Ac) as a marker for GER in a randomized, partially replicated, 6 × 6 Latin square design. Pre- and post-prandial blood samples were collected at 16 timepoints from −15 to 480 min. Serum Ac concentrations were assessed via standard spectrophotometric assays and fitted with a mathematical model to estimate parameters of GER. Parameter values were subjected to ANOVA, with period and treatment as fixed effects and dog as a random effect. More total emptying (p = 0.074) occurred at a faster rate (p = 0.028) in dogs fed the grain-free diet, which contained the lowest total starch (34.03 ± 0.23%) and highest resistant starch (0.52 ± 0.007%). This research may benefit future diet formulations to reduce the prevalence of canine weight gain.
Milk Production Responses and Digestibility of Dairy Buffaloes (Bubalus bubalis) Partially Supplemented with Forage Rape (Brassica napus) Silage Replacing Corn Silage
Authors: Zhou Di , Abdelrahman Mohamed , Zhang Xinxin , Yang Shuai , Yuan Jing , An Zhigao , Niu Kaifeng , Gao Yanxia , Li Jianguo , Wang Bo , Zhou Guangsheng , Yang Liguo , Hua Guohua
Published: October 2021

Worldwide, silage is considered the main component in dairy animal diets; however, this portion is mainly dominated by corn silage, which raises availability challenges in some agricultural production systems. The present study evaluated a partial replacement of corn silage with forage rape silage (FRS) and its effect on feed intake, nutrient digestibility, rumen fermentation, milk production, and blood metabolites in buffalo. Thirty-six lactating buffaloes were randomly assigned to four different groups, according to supplementation of FRS (only corn silage, FRS0) or with 15% (FRS15), 25% (FRS25), and 35% (FRS35) of forage rape silage instead of corn silage. The results showed that, compared to corn silage, forage rape silage has a lower carbohydrate but a higher protein concentration. The buffalo intake of dry matter and organic matter were improved linearly with the FRS increasing in the diet. The apparent total-tract digestibility (ATTD) of dry matter, organic matter, nitrogen, neutral detergent fiber, and acid detergent fiber also increased by the FRS supplementation compared with FRS0. Conversely, FRS supplementation decreased the propionic, butyric, and valeric acid contents and increased the acetic:propionic ratio and microbial protein content. Furthermore, FRS inclusion led to a significantly higher milk urea and non-fat milk solid content, higher blood glucose, total globulins, blood urea nitrogen, and lower blood high-density lipoprotein. These results suggested that FRS has high a nutritional value and digestibility, is a good feed resource, and showed favorable effects when supplemented with dairy buffalo ration.
Dietary Supplementation of Olive Mill Waste Water Polyphenols in Rabbits: Evaluation of the Potential Effects on Hepatic Apoptosis, Inflammation and Metabolism through RT-qPCR Approach
Authors: Cappelli Katia , Ferlisi Flavia , Mecocci Samanta , Maranesi Margherita , Trabalza-Marinucci Massimo , Zerani Massimo , Dal Bosco Alessandro , Acuti Gabriele
Published: October 2021

Agro-industrial processing for the production of food or non-food products generates a wide range of by-products and residues rich in bioactive compounds including polyphenols. The concentration of these by-products is sometimes higher than in the original raw material as in the case of olive mill waste water (OMWW), one of the main by-products of olive oil extraction. Polyphenols are secondary plant metabolites that regulate the expression of specific inflammatory genes, transcriptional factors and pro/anti-apoptotic molecules, thus modulating the signaling pathways essential for cell health and homeostasis. The liver plays a key role in regulating homeostasis by responding to dietary changes in order to maintain nutritional and physiological states. In this study a nutrigenomic approach was adopted, which focuses on the effects of diet–health–gene interactions and the modulation of cellular processes, in order to evaluate the expression of the genes (AGER, BAX, COX2, IL1B, PPARA, PPARG, SIRT1, TNFA) involved in these interactions in the livers of rabbits fed with a diet supplemented with OMWW (POL) or without supplements (control, CTR). The RT-qPCR analysis showed the down-regulation of SIRT1, TNFA, AGER, BAX and PPARA transcripts in the POL group compared to the CTR group. These results show that OMWW dietary supplementation prevents cell death and tissue deterioration in rabbits.
A Comparison of Two Different Slaughter Systems for Lambs. Effects on Carcass Characteristics, Technological Meat Quality and Sensory Attributes
Authors: Stenberg Elin , Arvidsson-Segerkvist Katarina , Karlsson Anders H. , Ólafsdóttir Aðalheiður , Hilmarsson Óli Þór , Gudjónsdóttir María , Thorkelsson Guðjón
Published: October 2021

Two slaughter systems for lambs and their effects on meat quality in terms of texture, colour and sensory attributes were compared. The slaughter systems differed in methods for controlling rigor mortis and carcass chilling. One slaughter system (large-scale) used electrical stimulation and fast chilling of carcasses, while the other system (small-scale) did not use electrical stimulation and applied slower chilling, with carcass temperature decreasing over a longer period after slaughter. Ten pairs of ram lamb twins were selected, and one of each pair was slaughtered at the large-scale abattoir and the other at the small-scale abattoir. Carcass weight, conformation, fatness, pH and temperature were recorded. Musculus longissimus thoracis et lumborum was analysed for colour, cooking loss, Warner–Bratzler shear force and sensory attributes. For meat quality attributes, the only differences were found in meat colour L* (lightness; p = 0.0073), sensory attribute “appearance colour” (p = 0.0089) and “fatty flavour” (p = 0.0554). Meat from the small-scale abattoir was darker in colour and had a more fatty flavour than the meat from the large-scale abattoir. For sensory attributes (apart from colour), no significant differences were found between the two abattoir systems.
A Comparison of Times Taken for the Placement of the First Portal and Complication Rates between the Veress Needle Technique and the Modified Hasson Technique in Canine Ovariectomy Laparoscopic Surgery
Authors: Bianchi Amanda , Collivignarelli Francesco , Vignoli Massimo , Scaletta Lorenzo , Cuomo Amedeo , Falerno Ilaria , Paolini Andrea , Tamburro Roberto
Published: October 2021

Minimally invasive surgery is increasingly being used in veterinary medicine. Laparoscopic procedures have several advantages compared with open surgery. These include the magnification of the field of surgery, reduced post-surgical pain and associated stress, reduced post-operative infection rates, and decreased hospitalization time. The establishment of a pneumoperitoneum is a critical step; however, this procedure can prolong the operation time, and most of the complications associated with laparoscopic surgery have been attributed to the insertion of devices into the abdominal cavity. Two main techniques have been employed to create pneumoperitoneum: the closed-entry method using the Veress needle and the open Hasson technique. The first portal is necessary to start insufflation and, subsequently, to realize the operative channel to insert the laparoscopic instruments into the abdomen. Many authors have compared the time necessary to create the first portal using different techniques in human medicine, but studies on this topic in veterinary medicine are lacking. In the veterinary medicine literature, complications associated with the creation of a pneumoperitoneum and the placement of ports include spleen, bowel, or bladder injuries; pneumothorax; and subcutaneous emphysema. The aim of the present study was to compare the times required for the placement of the first portal and the creation of pneumoperitoneum, and the rates of intraoperative complications using the Veress needle technique (VNT) and the open modified Hasson technique (MHT). The sample population comprised 30 female dogs who underwent laparoscopic ovariectomies. The dogs were randomly organized into two groups and two different entry techniques were used: Veress needle (VNT = group A) and the modified Hasson technique (MHT = group B). Complications related to abdominal entry were classified as major, in cases of organ perforation, and minor, in cases of subcutaneous emphysema and gas leakage. The VNT and MHT required 374.0 s and 242.9 s, respectively, for the placement of the first portal and for establishing pneumoperitoneum (p < 0.05). Their major complications rates were 20% and 0%, respectively (p < 0.05). Their minor complications rates were 20% and 35%, respectively (p < 0.05). No surgical procedures required laparotomy. The MHT was associated with a lower major complication rate and required less time to create the first portal, compared with the Veress needle technique.
Is Penicillin Allergy a Risk Factor for Early Dental Implant Failure? A Systematic Review
Authors: Salgado-Peralvo Angel-Orión , Peña-Cardelles Juan-Francisco , Kewalramani Naresh , Ortiz-García Iván , Jiménez-Guerra Álvaro , Uribarri Andrea , Velasco-Ortega Eugenio , Moreno-Muñoz Jesús , Núñez-Márquez Enrique , Monsalve-Guil Loreto
Published: October 2021

The prescription of preventive antibiotics in dental implant treatments reduces the incidence of early failures. This study has focused mainly on the influence of amoxicillin, which is contraindicated in penicillin-allergic patients. The present systematic review aimed to determine whether penicillin-allergic patients have a higher risk of implant failure compared to non-allergic patients. An electronic search was performed on Medline and Web of Science using the following MeSH terms: (penicillin allergy OR clindamycin OR erythromycin OR azithromycin OR metronidazole) AND (dental implant OR dental implant failure OR dental implant complications). The criteria employed were those described in the PRISMA® Declaration. Only five articles were included that analyzed the failure rates of implants placed in penicillin-allergic patients who were prescribed clindamycin compared to non-allergic patients who were prescribed amoxicillin. With the limitations of this study, it is not possible to state that penicillin allergy per se constitutes a risk factor for early dental implant failure as most of the studies included self-reported allergic patients. Clindamycin has been associated with a significantly elevated risk of failure and an up to six times increased risk of infection. Immediate implants also have a 5.7 to 10 times higher risk of failure.
Analytical Methods for Lipid Oxidation and Antioxidant Capacity in Food Systems
Authors: Abeyrathne Edirisingha Dewage Nalaka Sandun , Nam Kichang , Ahn Dong Uk
Published: October 2021

Lipid oxidation is the most crucial quality parameter in foods. Many methods were developed to determine the level of oxidation and antioxidant activity. This review compares the methods used to determine lipid oxidation and antioxidant capacity in foods. Lipid oxidation methods developed are based on the direct or indirect measurement of produced primary or secondary oxidation substances. Peroxide values and conjugated diene methods determine the primary oxidative products of lipid oxidation and are commonly used for plant oils and high-fat products. 2-Thiobarbituric acid-reactive substances and chromatographic methods are used to determine the secondary products of oxidation and are suitable for meat and meat-based products. The fluorometric and sensory analyses are indirect methods. The antioxidant capacity of additives is determined indirectly using the lipid oxidation methods mentioned above or directly based on the free-radical scavenging activity of the antioxidant compounds. Each lipid oxidation and antioxidant capacity methods use different approaches, and one method cannot be used for all foods. Therefore, selecting proper methods for specific foods is essential for accurately evaluating lipid oxidation or antioxidant capacity.
Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro
Authors: Lin His , Lin Ting-Yun , Lin Jer-An , Cheng Kuan-Chen , Santoso Shella Permatasari , Chou Chun-Hsu , Hsieh Chang-Wei
Published: October 2021

Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiaging-related skincare products.
Curcumin and Carnosic Acid Cooperate to Inhibit Proliferation and Alter Mitochondrial Function of Metastatic Prostate Cancer Cells
Authors: Ossikbayeva Saniya , Khanin Marina , Sharoni Yoav , Trachtenberg Aviram , Tuleukhanov Sultan , Sensenig Richard , Rom Slava , Danilenko Michael , Orynbayeva Zulfiya
Published: October 2021

Anticancer activities of plant polyphenols have been demonstrated in various models of neoplasia. However, evidence obtained in numerous in vitro studies indicates that proliferation arrest and/or killing of cancer cells require quite high micromolar concentrations of polyphenols that are difficult to reach in vivo and can also be (geno)toxic to at least some types of normal cells. The ability of certain polyphenols to synergize with one another at low concentrations can be used as a promising strategy to effectively treat human malignancies. We have recently reported that curcumin and carnosic acid applied at non-cytotoxic concentrations synergistically cooperate to induce massive apoptosis in acute myeloid leukemia cells, but not in normal hematopoietic and non-hematopoietic cells, via sustained cytosolic calcium overload. Here, we show that the two polyphenols can also synergistically suppress the growth of DU145 and PC-3 metastatic prostate cancer cell cultures. However, instead of cell killing, the combined treatment induced a marked inhibition of cell proliferation associated with G0/G1 cell cycle arrest. This was preceded by transient elevation of cytosolic calcium levels and prolonged dissipation of the mitochondrial membrane potential, without generating oxidative stress, and was associated with defective oxidative phosphorylation encompassing mitochondrial dysfunction. The above effects were concomitant with a significant downregulation of mRNA and protein expression of the oncogenic kinase SGK1, the mitochondria-hosted mTOR component. In addition, a moderate decrease in SGK1 phosphorylation at Ser422 was observed in polyphenol-treated cells. The mTOR inhibitor rapamycin produced a similar reduction in SGK1 mRNA and protein levels as well as phosphorylation. Collectively, our findings suggest that the combination of curcumin and carnosic acid at potentially bioavailable concentrations may effectively target different types of cancer cells by distinct modes of action. This and similar combinations merit further exploration as an anticancer modality.
Real-Time Detection Method for Center and Attitude Precise Positioning of Cross Laser-Pattern
Authors: Li Haopeng , Qiu Zurong , Jiang Haodan
Published: October 2021

Optical metrology has experienced a fast development in recent years—cross laser-pattern has become a common cooperative measuring marker in optical metrology equipment, such as infrared imaging equipment or visual 3D measurement system. The rapid and accurate extraction of the center point and attitude of the cross-marker image is the first prerequisite to ensure the measurement speed and accuracy. In this paper, a cross laser-pattern is used as a cooperative marker, in view of the high resolution of the cross laser-pattern image in the project and the vulnerability to adverse environmental effects, such as stray light, smoke, water mist and other interference in the environment, resulting in poor contrast, low signal-to-noise ratio (SNR), uneven energy distribution. As a result, a method is proposed to detect the center point and attitude of cross laser-pattern image based on Gaussian fitting and least square fitting. Firstly, the distortion of original image is corrected in real time, the corrected image is smoothed by median filter, and the noise is suppressed while preserving the edge sharpness and detail of the image. In order to adapt to different environments, the maximum inter-class variance method of threshold automatic selection is used to determine the threshold of image segmentation to eliminate the background interference caused by different illumination intensities. To improve the real-time performance of the algorithm, the four cross laser edge pixels are obtained by line search, and then fitted by least square. With the edge lines, the transverse and portrait line of the cross-laser image are separated, then we calculate Gaussian center points of all Gaussian sections of transverse and portrait lines based on Gaussian fitting method, respectively. Based on the traditional line fitting method, the sub-pixel center of the transverse and portrait laser strip images are fitted by removing the Outlying Points, and the center coordinates and attitude information of the cross laser-pattern are calculated by using the center equation of the laser strip, realizing cross laser-pattern center and attitude accurate positioning. The results show that the method is robust, the center positioning accuracy is better than 0.6 pixels, the attitude positioning accuracy is better than ±15” under smoke and water mist environment and the processing speed is better than 0.1 s, which meets the real-time requirements of the project.
The Effectiveness of Dental Protection and the Material Arrangement in Custom-Made Mouthguards
Authors: Messias Ana , Gomes Inês J. , Reis Paulo N. B. , Amaro Ana M. , Neto Maria A.
Published: October 2021

Experimental research studies have shown that wearing a mouthguard (MG) is an effective way to prevent tooth or maxillofacial trauma. However, there is a lack of scientific information regarding how the material arrangement within the mouthguard can modify its mechanical response during an impact. Hence, this study aimed to evaluate the influence of material arrangement within custom-made mouthguards on stress transmitted to anterior teeth, bone, and soft tissue after impact. Four 3D finite element models of a human maxilla were reconstructed based on the CBCT of a young patient and analyzed according to the presence or absence of a mouthguard and the type of material arrangement within those with a mouthguard: model NMG with no mouthguard; model CMG representing the conventional arrangement with a single 4 mm-thick ethylene-vinyl acetate (EVA) foil; model FMG presenting layer arrangement with two 1 mm-thick foils of EVA in the outer shell and one 2 mm-thick foil of EVA foam in the core; model HMG presenting a 1 mm-thick compact inner and outer shell of EVA and a 2 mm wide air-filled zone in the core. Linear quasi-static analysis and frontal load were used to simulate an impact with an energy of 4.4 J. Isotropic linear elastic properties were assumed for the bone and teeth but not for the mouthguard protection and oral soft tissues. The results were evaluated and compared in terms of displacement, stretches, and stresses. All the mouthguards analyzed reduced the risk of injury to teeth and bone, reducing the displacement and stress of these structures. However, the implementation of a honeycomb structured layer allowed more significant displacement and deformation of the mouthguard’s external layer, thus promoting higher protection of the anatomic structures, namely the root dentin and the bone tissue. Nevertheless, the results also indicate that improving the mouthguard flexibility might increase the soft tissue injuries.
Nanoclay and Polystyrene Type Efficiency on the Development of Polystyrene/Montmorillonite/Oregano Oil Antioxidant Active Packaging Nanocomposite Films
Authors: Giannakas Aris E. , Salmas Constantinos E. , Karydis-Messinis Andreas , Moschovas Dimitrios , Kollia Eleni , Tsigkou Vasiliki , Proestos Charalampos , Avgeropoulos Apostolos , Zafeiropoulos Nikolaos E.
Published: October 2021

Over the years, there has been an effort to extend food shelf life so as to reduce global food waste. The use of natural biodegradable materials in production procedures is more and more adopted nowadays in order to achieve cyclic economy targets and improve environmental and human health indexes. Active packaging is the latest trend for food preservation. In this work, polystyrene was mixed with natural NaMt, OrgNaMt montmorillonite, and oregano essential oil to develop a new packaging film. Strength, oxygen and water-vapour permeation, blending and homogeneity, and antimicrobial and antioxidant activity were measured as basic parameters for food packaging films characterization. Instruments such as a tensile measurement instrument, XRD, FTIR, DMA, OPA (Oxygen Permeation Analyzer), and other handmade devices were used. Results showed that polystyrene could be modified, improved, and exhibits food odour prevention characteristics in order to be used for applications on food active packaging. The material with the code name PS5OO@OrgMt qualified between the tested samples as the most promising material for food active packaging applications.
Systematic Literature Review of Challenges in Blockchain Scalability
Authors: Khan Dodo , Jung Low Tang , Hashmani Manzoor Ahmed
Published: October 2021

Blockchain technology is fast becoming the most transformative technology of recent times and has created hype and optimism, gaining much attention from the public and private sectors. It has been widely deployed in decentralized crypto currencies such as Bitcoin and Ethereum. Bitcoin is the success story of a public blockchain application that propelled intense research and development into blockchain technology. However, scalability remains a crucial challenge. Both Bitcoin and Ethereum are encountering low-efficiency issues with low throughput, high transaction latency, and huge energy consumption. The scalability issue in public Blockchains is hindering the provision of optimal solutions to businesses and industries. This paper presents a systematic literature review (SLR) on the public blockchain scalability issue and challenges. The scope of this SLR includes an in-depth investigation into the scalability problem of public blockchain, associated fundamental factors, and state-of-art solutions. This project managed to extract 121 primary papers from major scientific databases such as Scopus, IEEE explores, Science Direct, and Web of Science. The synthesis of these 121 articles revealed that scalability in public blockchain is not a singular term. A variety of factors are allied to it, with transaction throughput being the most discussed factor. In addition, other interdependent vita factors include storages, block size, number of nodes, energy consumption, latency, and cost. Generally, each term is somehow directly or indirectly reliant on the consensus model embraced by the blockchain nodes. It is also noticed that the contemporary available consensus models are not efficient in scalability and thus often fail to provide good QoS (throughput and latency) for practical industrial applications. Our findings exemplify that the Internet of Things (IoT) would be the leading application of blockchain in industries such as energy, finance, resource management, healthcare, education, and agriculture. These applications are, however, yet to achieve much-desired outcomes due to scalability issues. Moreover, Onchain and offchain are the two major categories of scalability solutions. Sagwit, block size expansion, sharding, and consensus mechanisms are examples of onchain solutions. Offchain, on the other hand, is a lighting network.