bluuebunny/crossref_metadata_2025 huggingface dataset:
Fishing Innate Immune System Properties through the Transcriptomic Single-Cell Data of Teleostei
Authors: Bobrovskikh Aleksandr V. , Zubairova Ulyana S. , Doroshkov Alexey V.
Published: December 2023

The innate immune system is the first line of defense in multicellular organisms. Danio rerio is widely considered a promising model for IIS-related research, with the most amount of scRNAseq data available among Teleostei. We summarized the scRNAseq and spatial transcriptomics experiments related to the IIS for zebrafish and other Teleostei from the GEO NCBI and the Single-Cell Expression Atlas. We found a considerable number of scRNAseq experiments at different stages of zebrafish development in organs such as the kidney, liver, stomach, heart, and brain. These datasets could be further used to conduct large-scale meta-analyses and to compare the IIS of zebrafish with the mammalian one. However, only a small number of scRNAseq datasets are available for other fish (turbot, salmon, cavefish, and dark sleeper). Since fish biology is very diverse, it would be a major mistake to use zebrafish alone in fish immunology studies. In particular, there is a special need for new scRNAseq experiments involving nonmodel Teleostei, e.g., long-lived species, cancer-resistant fish, and various fish ecotypes.
Comparative Transcriptome Analysis of the Response to Vibrio parahaemolyticus and Low-Salinity Stress in the Swimming Crab Portunus trituberculatus
Authors: Sun Dongfang , Lv Jianjian , Li Yukun , Wu Jie , Liu Ping , Gao Baoquan
Published: December 2023

Vibrio parahaemolyticus is one of the main pathogenic bacteria of Portunus trituberculatus and causes mass mortality of P. trituberculatus in aquaculture. In addition, low-salinity stimulation makes P. trituberculatus more susceptible to V. parahaemolyticus infections. In order to elucidate the molecular mechanism of resistance to V. parahaemolyticus in P. trituberculatus, comparative transcriptomic analysis of blood cells stimulated by low salinity and V. parahaemolyticus was carried out in this study. Transcriptome sequencing of low-salinity stress and pathogen infection at different time points was completed using Illumina sequencing technology. A total of 5827, 6432, 5362 and 1784 differentially expressed genes (DEGs) involved in pathways related to ion transport and immunoregulation were found under low-salinity stress at 12, 24, 48 and 72 h compared with the control at 0 h. In contrast, 4854, 4814, 5535 and 6051 DEGs, which were significantly enriched in Toll and IMD signaling pathways, were found at 12, 24, 48 and 72 h compared with the control at 0 h under V. parahaemolyticus infection. Among them, 952 DEGs were shared in the two treatment groups, which were mainly involved in apoptosis and Hippo signaling pathway. Cluster analysis screened 103 genes that were differentially expressed in two factors that were negatively correlated, including immunoglobulin, leukocyte receptor cluster family, scavenger receptor, macroglobulin and other innate-immune-related genes. These results provide data support for the analysis of the mechanisms of immunity to V. parahaemolyticus under low-salinity stress in P. trituberculatus and help to elucidate the molecular mechanisms by which environmental factors affect immunity.
Myostatin and the Heart
Authors: Knapp Małgorzata , Supruniuk Elżbieta , Górski Jan
Published: December 2023

Myostatin (growth differentiation factor 8) is a member of the transforming growth factor-β superfamily. It is secreted mostly by skeletal muscles, although small amounts of myostatin are produced by the myocardium and the adipose tissue as well. Myostatin binds to activin IIB membrane receptors to activate the downstream intracellular canonical Smad2/Smad3 pathway, and additionally acts on non-Smad (non-canonical) pathways. Studies on transgenic animals have shown that overexpression of myostatin reduces the heart mass, whereas removal of myostatin has an opposite effect. In this review, we summarize the potential diagnostic and prognostic value of this protein in heart-related conditions. First, in myostatin-null mice the left ventricular internal diameters along with the diastolic and systolic volumes are larger than the respective values in wild-type mice. Myostatin is potentially secreted as part of a negative feedback loop that reduces the effects of the release of growth-promoting factors and energy reprogramming in response to hypertrophic stimuli. On the other hand, both human and animal data indicate that myostatin is involved in the development of the cardiac cachexia and heart fibrosis in the course of chronic heart failure. The understanding of the role of myostatin in such conditions might initiate a development of targeted therapies based on myostatin signaling inhibition.
Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection
Authors: Huysseune Ann , Larsen Ulrike G. , Larionova Daria , Matthiesen Cecilie L. , Petersen Steen V. , Muller Marc , Witten P. Eckhard
Published: December 2023

DAF-FM DA is widely used as a live staining compound to show the presence of nitric oxide (NO) in cells. Applying this stain to live zebrafish embryos is known to indicate early centers of bone formation, but the precise (cellular) location of the signal has hitherto not been revealed. Using sections of zebrafish embryos live-stained with DAF-FM DA, we could confirm that the fluorescent signals were predominantly located in areas of ongoing bone formation. Signals were observed in the bone and tooth matrix, in the notochord sheath, as well as in the bulbus arteriosus. Surprisingly, however, they were exclusively extracellular, even after very short staining times. Von Kossa and Alizarin red S staining to reveal mineral deposits showed that DAF-FM DA stains both the mineralized and non-mineralized bone matrix (osteoid), excluding that DAF-FM DA binds non-specifically to calcified structures. The importance of NO in bone formation by osteoblasts is nevertheless undisputed, as shown by the absence of bone structures after the inhibition of NOS enzymes that catalyze the formation of NO. In conclusion, in zebrafish skeletal biology, DAF-FM DA is appropriate to reveal bone formation in vivo, independent of mineralization of the bone matrix, but it does not demonstrate intracellular NO.
Resilience and Vulnerability to Stress-Induced Anhedonia: Unveiling Brain Gene Expression and Mitochondrial Dynamics in a Mouse Chronic Stress Depression Model
Authors: Strekalova Tatyana , Svirin Evgeniy , Gorlova Anna , Sheveleva Elizaveta , Burova Alisa , Khairetdinova Adel , Sitdikova Kseniia , Zakharova Elena , Dudchenko Alexander M. , Lyundup Aleksey , Morozov Sergey
Published: December 2023

The role of altered brain mitochondrial regulation in psychiatric pathologies, including Major Depressive Disorder (MDD), has attracted increasing attention. Aberrant mitochondrial functions were suggested to underlie distinct inter-individual vulnerability to stress-related MDD syndrome. In this context, insulin receptor sensitizers (IRSs) that regulate brain metabolism have become a focus of recent research, as their use in pre-clinical studies can help to elucidate the role of mitochondrial dynamics in this disorder and contribute to the development of new antidepressant treatment. Here, following 2-week chronic mild stress (CMS) using predation, social defeat, and restraint, MDD-related behaviour and brain molecular markers have been investigated along with the hippocampus-dependent performance and emotionality in mice that received the IRS dicholine succinate (DS). In a sucrose test, mice were studied for the key feature of MDD, a decreased sensitivity to reward, called anhedonia. Based on this test, animals were assigned to anhedonic and resilient-to-stress-induced-anhedonia groups, using a previously established criterion of a decrease in sucrose preference below 65%. Such assignment was based on the fact that none of control, non-stressed animals displayed sucrose preference that would be smaller than this value. DS-treated stressed mice displayed ameliorated behaviours in a battery of assays: sucrose preference, coat state, the Y-maze, the marble test, tail suspension, and nest building. CMS-vulnerable mice exhibited overexpression of the inflammatory markers Il-1β, tnf, and Cox-1, as well as 5-htt and 5-ht2a-R, in various brain regions. The alterations in hippocampal gene expression were the closest to clinical findings and were studied further. DS-treated, stressed mice showed normalised hippocampal expression of the plasticity markers Camk4, Camk2, Pka, Adcy1, Creb-ar, Nmda-2r-ar, and Nmda-2r-s. DS-treated and non-treated stressed mice who were resilient or vulnerable to anhedonia were compared for hippocampal mitochondrial pathway regulation using Illumina profiling. Resilient mice revealed overexpression of the mitochondrial complexes NADH dehydrogenase, succinate dehydrogenase, cytochrome bc1, cytochrome c oxidase, F-type and V-type ATPases, and inorganic pyrophosphatase, which were decreased in anhedonic mice. DS partially normalised the expression of both ATPases. We conclude that hippocampal reduction in ATP synthesis is associated with anhedonia and pro-inflammatory brain changes that are ameliorated by DS.
Ethnobotany, Phytochemistry, and Biological Activities of the Genus Cordyline
Authors: Tematio Fouedjou Romuald , Tsakem Bienvenu , Siwe-Noundou Xavier , Dongmo Fogang Hervet P. , Tiombou Donkia Aphalaine , Kemvoufo Ponou Beaudelaire , Poka Madan , Demana Patrick H. , Teponno Rémy B. , Azefack Tapondjou Léon
Published: December 2023

Cordyline species have a long history in traditional medicine as a basis of treatment for various ailments such as a bloody cough, dysentery, and a high fever. There are about 26 accepted species names in this genus distributed worldwide, including C. fruticosa, C. autralis, C. stricta, C. cannifolia, and C. dracaenosides. This work presents a comprehensive review of the traditional uses of plants of the genus Cordylie and their chemical constituents and biological activities. A bibliographic search was conducted to identify available information on ethnobotany, ethnopharmacology, chemical composition, and biological activities. A total of 98 isolated compounds potentially responsible for most of the traditional medicinal applications have been reported from eight species of Cordyline and are characterised as flavonoid, spirostane, furostane, and cholestane glycosides. Some of these pure compounds, as well as extracts from some species of Cordyline, have exhibited noteworthy anti-oxidant, antiproliferative, antimicrobial, and hypolipidemic activities. Although many of these species have not yet been investigated phytochemically or pharmacologically, they remain a potential source of new bioactive compounds.
Erucin Exerts Cardioprotective Effects on Ischemia/Reperfusion Injury through the Modulation of mitoKATP Channels
Authors: Flori Lorenzo , Montanaro Rosangela , Pagnotta Eleonora , Ugolini Luisa , Righetti Laura , Martelli Alma , Di Cesare Mannelli Lorenzo , Ghelardini Carla , Brancaleone Vincenzo , Testai Lara , Calderone Vincenzo
Published: December 2023

Modulation of mitochondrial K channels represents a pharmacological strategy to promote cardioprotective effects. Isothiocyanates emerge as molecules capable of releasing hydrogen sulfide (H2S), an endogenous pleiotropic gasotransmitter responsible for anti-ischemic cardioprotective effects also through the involvement of mitoK channels. Erucin (ERU) is a natural isothiocyanate resulting from the enzymatic hydrolysis of glucosinolates (GSLs) present in Eruca sativa Mill. seeds, an edible plant of the Brassicaceae family. In this experimental work, the specific involvement of mitoKATP channels in the cardioprotective effect induced by ERU was evaluated in detail. An in vivo preclinical model of acute myocardial infarction was reproduced in rats to evaluate the cardioprotective effect of ERU. Diazoxide was used as a reference compound for the modulation of potassium fluxes and 5-hydroxydecanoic acid (5HD) as a selective blocker of KATP channels. Specific investigations on isolated cardiac mitochondria were carried out to evaluate the involvement of mitoKATP channels. The results obtained showed ERU cardioprotective effects against ischemia/reperfusion (I/R) damage through the involvement of mitoKATP channels and the consequent depolarizing effect, which in turn reduced calcium entry and preserved mitochondrial integrity.
Unraveling Down Syndrome: From Genetic Anomaly to Artificial Intelligence-Enhanced Diagnosis
Authors: Koul Aabid Mustafa , Ahmad Faisel , Bhat Abida , Aein Qurat-ul , Ahmad Ajaz , Reshi Aijaz Ahmad , Kaul Rauf-ur-Rashid
Published: December 2023

Down syndrome arises from chromosomal non-disjunction during gametogenesis, resulting in an additional chromosome. This anomaly presents with intellectual impairment, growth limitations, and distinct facial features. Positive correlation exists between maternal age, particularly in advanced cases, and the global annual incidence is over 200,000 cases. Early interventions, including first and second-trimester screenings, have improved DS diagnosis and care. The manifestations of Down syndrome result from complex interactions between genetic factors linked to various health concerns. To explore recent advancements in Down syndrome research, we focus on the integration of artificial intelligence (AI) and machine learning (ML) technologies for improved diagnosis and management. Recent developments leverage AI and ML algorithms to detect subtle Down syndrome indicators across various data sources, including biological markers, facial traits, and medical images. These technologies offer potential enhancements in accuracy, particularly in cases complicated by cognitive impairments. Integration of AI and ML in Down syndrome diagnosis signifies a significant advancement in medical science. These tools hold promise for early detection, personalized treatment, and a deeper comprehension of the complex interplay between genetics and environmental factors. This review provides a comprehensive overview of neurodevelopmental and cognitive profiles, comorbidities, diagnosis, and management within the Down syndrome context. The utilization of AI and ML represents a transformative step toward enhancing early identification and tailored interventions for individuals with Down syndrome, ultimately improving their quality of life.
Leukocyte- and Platelet-Rich Fibrin (L-PRF) Obtained from Smokers and Nonsmokers Shows a Similar Uniaxial Tensile Response In Vitro
Authors: Lara Cesar , Bezmalinovic Alejandro , García-Herrera Claudio , Ríos Susana , Valenzuela Loreto M. , Martínez Constanza E.
Published: December 2023

We evaluated and compared the biomechanical properties of Leukocyte-and Platelet Rich Fibrin L-PRF clots and membranes derived from smoker and nonsmoker donors. Twenty venous-blood donors (aged 18 to 50 years) were included after signing informed consent forms. L-PRF clots were analyzed and then compressed to obtain L-PRF membranes. L-PRF clot and membrane samples were tested in quasi-static uniaxial tension and the stress–stretch response was registered and characterized. Furthermore, scanning electron microscope representative images were taken to see the fibrin structure from both groups. The analysis of stress–stretch curves allowed us to evaluate the statistical significance in differences between smoker and nonsmoker groups. L-PRF membranes showed a stiffer response and higher tensile strength when compared to L-PRF clots. However, no statistically significant differences were found between samples from smokers and nonsmokers. With the limitations of our in vitro study, we can suggest that the tensile properties of L-PRF clots and membranes from the blood of smokers and nonsmokers are similar. More studies are necessary to fully characterize the effect of smoking on the biomechanical behavior of this platelet concentrate, to further encourage its use as an alternative to promote wound healing in smokers.
Estimating the Prevalence and Characteristics of Patients Potentially Eligible for Lipoprotein(a)-Lowering Therapies in a Real-World Setting
Authors: Cicero Arrigo F. G. , Fogacci Federica , Giovannini Marina , Grandi Elisa , D’Addato Sergio , Borghi Claudio
Published: December 2023

High lipoprotein(a) (Lp(a)) plasma levels are significantly associated with an increased risk of developing atherosclerotic cardiovascular diseases (ASCVD). The aim of this analysis was to estimate the prevalence and characteristics of patients potentially eligible for Lp(a)-lowering therapies in a real-world setting (i.e., patients with ASCVD and Lp(a) levels > 70 mg/dL). For this reason, we pooled data from a large cohort of Italian outpatients (N = 5961; men: 2879, women: 3982) with dyslipidemia. A binary logistic regression analysis was used to determine the significant predictors of ASCVD in the cohort, which were age (Odds Ratio (OR): 1.158, 95% Confidence Interval (CI): 1.114 to 1.203, p < 0.001), low-density lipoprotein cholesterol at entry (OR: 1.989, 95% CI: 1.080 to 1.198, p = 0.020) and Lp(a) (OR: 1.090, 95% CI: 1.074 to 1.107, p < 0.001). In our cohort, almost half of patients with ASCVD (44.7%) may be eligible to be treated with Lp(a)-lowering agents. Interestingly, patients who do not meet the treatment criteria despite high Lp(a) (50–70 mg/dL), respectively, account for 4.7% and 7.3% of those in primary and secondary ASCVD prevention. In conclusion, in our large cohort of outpatients with dyslipidemia, the prevalence of individuals with ASCVD and very high Lp(a) plasma levels is quite high, even with a conservative estimation.
Robust Superhydrophobicity through Surface Defects from Laser Powder Bed Fusion Additive Manufacturing
Authors: Kan Longxin , Zhang Lei , Wang Pengfei , Liu Qi , Wang Jihao , Su Bin , Song Bo , Shi Yusheng
Published: December 2023

The robustness of superhydrophobic objects conflicts with both the inevitable introduction of fragile micro/nanoscale surfaces and three-dimensional (3D) complex structures. The popular metal 3D printing technology can manufacture robust metal 3D complex components, but the hydrophily and mass surface defects restrict its diverse application. Herein, we proposed a strategy that takes the inherent ridges and grooves’ surface defects from laser powder bed fusion additive manufacturing (LPBF-AM), a metal 3D printing process, as storage spaces for hydrophobic silica (HS) nanoparticles to obtain superhydrophobic capacity and superior robustness. The HS nanoparticles stored in the grooves among the laser-melted tracks serve as the hydrophobic guests, while the ridges’ metal network provides the mechanical strength, leading to robust superhydrophobic objects with desired 3D structures. Moreover, HS nanoparticles coated on the LPBF-AM-printed surface can inhibit corrosion behavior caused by surface defects. It was found that LPBF-AM-printed objects with HS nanoparticles retained superior hydrophobicity after 150 abrasion cycles (~12.5 KPa) or 50 cycles (~37.5 KPa). Furthermore, LPBF-AM-printed ships with superhydrophobic coating maintained great water repellency even after 10,000 cycles of seawater swashing, preventing dynamic corrosion upon surfaces. Our proposed strategy, therefore, provides a low-cost, highly efficient, and robust superhydrophobic coating, which is applicable to metal 3D architectures toward corrosion-resistant requirements.
TCP Doped with Metal Ions Reinforced with Tetragonal and Cubic Zirconia
Authors: Ferro Vanessa M. , Silva Beatriz C. , Macedo Duarte F. , Fernandes Natanael F. , Silva Abílio P.
Published: December 2023

Ceramic biocomposites based on bioactive tricalcium phosphate doped with metal ions are a strategy for obtaining good biomimetics for human bone composition. Manufacturing with PMMA porogen also induces bone-like porosity morphology. The poor strength of tricalcium phosphate can be overcomed by designing ceramic composites reinforced with tetragonal and cubic zirconia. In this work, five different bioceramic composites were manufactured without and with induced porosity and their physical, mechanical, microstructural, and biological properties were studied. With the addition of tetragonal and cubic zirconia, an improvement in strength of 22% and 55%, respectively, was obtained, corresponding to up to 20.7 MPa. PMMA was suitable for adding porosity, up to 30%, with interconnectivity while an excellent hOB cellular viability was achieved for all biocomposites.
The Body in Neurosciences: Representation, Perception and Space Processing
Authors: Palermo Liana , Boccia Maddalena
Published: December 2023

The Special Issue “The Body in Neurosciences: Representation, Perception and Space Processing” deals with the understanding of body processing in terms of the multisensorial perception of bodily related information, interoception, and mental representation, as well as its relationship with the peripersonal, interpersonal, and extrapersonal spaces, integrating findings from normal and pathological functioning [...]
Polypathologies and Animal Models of Traumatic Brain Injury
Authors: Freeman-Jones Erin , Miller William H. , Work Lorraine M. , Fullerton Josie L.
Published: December 2023

Traumatic brain injury (TBI) is an important health issue for the worldwide population, as it causes long-term pathological consequences for a diverse group of individuals. We are yet to fully elucidate the significance of TBI polypathologies, such as neuroinflammation and tau hyperphosphorylation, and their contribution to the development of chronic traumatic encephalopathy (CTE) and other neurological conditions. To advance our understanding of TBI, it is necessary to replicate TBI in preclinical models. Commonly used animal models include the weight drop model; these methods model human TBI in various ways and in different animal species. However, animal models have not demonstrated their clinical utility for identifying therapeutic interventions. Many interventions that were successful in improving outcomes for animal models did not translate into clinical benefit for patients. It is important to review current animal models and discuss their strengths and limitations within a TBI context. Modelling human TBI in animals encounters numerous challenges, yet despite these barriers, the TBI research community is working to overcome these difficulties. Developments include advances in biomarkers, standardising, and refining existing models. This progress will improve our ability to model TBI in animals and, therefore, enhance our understanding of TBI and, potentially, how to treat it.
Unveiling the Hidden Challenges: Non-Motor Disorders in Parkinson’s Disease
Authors: Nieto-Escamez Francisco , Obrero-Gaitán Esteban , García-López Héctor , Cortés-Pérez Irene
Published: December 2023

Parkinson’s disease (PD) is not just a motor disorder, it is a complex condition that affects every aspect of a patient’s life, from cognitive impairment and psychiatric disturbances to autonomic dysfunction and sleep disturbances [...]
Gestures as Scaffolding to Learn Vocabulary in a Foreign Language
Authors: García-Gámez Ana Belén , Macizo Pedro
Published: December 2023

This paper investigates the influence of gestures on foreign language (FL) vocabulary learning. In this work, we first address the state of the art in the field and then delve into the research conducted in our lab (three experiments already published) in order to finally offer a unified theoretical interpretation of the role of gestures in FL vocabulary learning. In Experiments 1 and 2, we examined the impact of gestures on noun and verb learning. The results revealed that participants exhibited better learning outcomes when FL words were accompanied by congruent gestures compared to those from the no-gesture condition. Conversely, when meaningless or incongruent gestures were presented alongside new FL words, gestures had a detrimental effect on the learning process. Secondly, we addressed the question of whether or not individuals need to physically perform the gestures themselves to observe the effects of gestures on vocabulary learning (Experiment 3). Results indicated that congruent gestures improved FL word recall when learners only observed the instructor’s gestures (“see” group) and when they mimicked them (“do” group). Importantly, the adverse effect associated with incongruent gestures was reduced in the “do” compared to that in the “see” experimental group. These findings suggest that iconic gestures can serve as an effective tool for learning vocabulary in an FL, particularly when the gestures align with the meaning of the words. Furthermore, the active performance of gestures helps counteract the negative effects associated with inconsistencies between gestures and word meanings. Consequently, if a choice must be made, an FL learning strategy in which learners acquire words while making gestures congruent with their meaning would be highly desirable.
Model Predictive Control for Energy Optimization of HVAC Systems Using EnergyPlus and ACO Algorithm
Authors: Bamdad Keivan , Mohammadzadeh Navid , Cholette Michael , Perera Srinath
Published: December 2023

The deployment of model-predictive control (MPC) for a building’s energy system is a challenging task due to high computational and modeling costs. In this study, an MPC controller based on EnergyPlus and MATLAB is developed, and its performance is evaluated through a case study in terms of energy savings, optimality of solutions, and computational time. The MPC determines the optimal setpoint trajectories of supply air temperature and chilled water temperature in a simulated office building. A comparison between MPC and rule-based control (RBC) strategies for three test days showed that the MPC achieved 49.7% daily peak load reduction and 17.6% building energy savings, which were doubled compared to RBC. The MPC optimization problem was solved multiple times using the Ant Colony Optimization (ACO) algorithm with different starting points. Results showed that ACO consistently delivered high-quality optimized control sequences, yielding less than a 1% difference in energy savings between the worst and best solutions across all three test days. Moreover, the computational time for solving the MPC problem and obtaining nearly optimal control sequences for a three-hour prediction horizon was observed to be around 22 min. Notably, reasonably good solutions were attained within 15 min by the ACO algorithm.
Experimental Study on Activation Energy and Microstructure of Nano- and Micro-Sized Pozzolanic Materials as Cementitious Composite Binder
Authors: Kim Won-Woo , Moon Jae-Heum
Published: December 2023

Silicate-based nano- and micro-sized binders were used with ordinary Portland cement to evaluate their influence on the setting time, activation energy, mechanical properties, and microstructure. It was found that the setting time was reduced due to the pozzolanic reaction of the silicate-based binders and the densification of the microstructure. However, there is a lack of research on nano-sized pozzolanic materials. Therefore, in this study, research on activation energy and microstructure was conducted. The compressive strength increased owing to a reduction in the porosity in the microstructure, and activation energy also tended to decrease. Moreover, using both micro-silica and a small proportion of nano-silica was more effective in reducing the setting time and activation energy than using any of them individually. The study established that adding a small proportion of nano-silica could reduce the setting time and increase the compressive strength because it positively influenced the pozzolanic reaction and filled the pores between micro-silica and cement, which were composed of relatively larger particles, with smaller particles. Because nanomaterials may degrade flowability due to their large specific surface area, it is deemed necessary to consider the addition of chemical admixtures during mix design. A characteristic has been revealed when nanomaterials are used, and special attention to the particle size distribution characteristics is required because the imbalance in particle size distribution may increase the porosity inside the microstructure. Therefore, it is recommended to use micro-sized pozzolanic materials together when using nano-sized pozzolanic materials.
A Review on Numerical Modeling of the Hygrothermal Behavior of Building Envelopes Incorporating Phase Change Materials
Authors: Sawadogo Mohamed , Godin Alexandre , Duquesne Marie , Hamami Ameur El Amine , Belarbi Rafik
Published: December 2023

Buildings are submitted to various external and internal solicitations that could affect its energy performance. Among these solicitations, temperature and moisture play a crucial role and could irrevocably affect the comfort of the occupants and the indoor air quality of the living environment. To assess the impact of the solicitation on building performance, a precise modeling of the heat, air, and moisture transfer phenomenon is necessary. This work proposes an extensive review of the hygrothermal models for building envelopes. The different models are divided into nodal and HAM techniques for heat, air, and moisture (HAM) transfer models. The HAM approach has been classified based on four driving potentials: moisture content, relative humidity, capillary pressure, and vapor pressure. Phase change materials (PCMs), alongside hygroscopic materials, enhance building thermal capacity and energy efficiency. There are various approaches to studying phase changes, with enthalpy-based and heat capacity approaches being the most popular. Building performance can be improved by combining PCM thermal inertia with hygroscopic moisture management. This review has exhibited the need for numerical models that address phase change and moisture behavior in these hybrid materials, capable of controlling temperature and humidity.
Evaluation of Occupational Safety in Restoration Projects of Historic Buildings: Risk Analysis with Selected Projects
Authors: Gümürçinler Tuğçe , Akboğa-Kale Özge
Published: December 2023

Restoration works in historical buildings, which have gained importance in Türkiye in recent years, have unique risks in terms of occupational safety. Employees in this sector are exposed to many different risks during the restoration of historical building projects, and ensuring occupational safety is an important problem for the sector. In order to find a solution to this problem, a comprehensive examination of historical building projects was made in terms of occupational safety during the restoration process. Within the scope of the study every stage of the three different restoration projects was examined in terms of occupational safety. In line with this research, job safety analysis was performed by dividing the steps in the restoration projects into groups. The risks in the construction sites were determined and precautions were presented to eliminate or reduce these risks. As a result, the risks that may occur during the restoration phase of a historical building are shown in detail together with the prevention methods. It is expected that if the stakeholders of restoration projects integrate the information presented into their occupational health and safety systems, it will contribute to the improvement in the processes and there will be a decrease in the frequency of work accidents.
Pediatric Atypical Melanocytic Proliferations: Single-Site Retrospective Cohort Assessment of Treatment and Long-Term Follow-Up
Authors: Hatheway Marshall Emily , Alvarez Gabriella , Wang Bangchen , Crimmins Jennifer , Schneider Michelle M. , Selim M. Angelica , Al-Rohil Rami N.
Published: December 2023

Atypical and malignant cutaneous tumors are understudied in the pediatric population, with limited data on long-term follow-up. This study examines pediatric (0–18 years) atypical melanocytic proliferations over a twenty-year period (January 2002–December2022) using the EPIC SlicerDicer at our institution. Over a twenty-year period, there were 55 cases of pediatric melanoma (53 patients). The median follow-up time was 8 years, 11 months. A proportion of 96% were treated with wide local excision (WLE), and 47% had a sentinel lymph node biopsy (SLNB) (35% positive rate). There were 101 atypical Spitz tumor cases (85% atypical Spitz tumors, 15% Spitz melanoma), with a median follow-up duration of 9 years. A proportion of 77% were treated with WLE (with one patient dying of metastatic disease). There were 10 cases of atypical melanocytic proliferations not otherwise specified, including 5 pigmented epithelioid melanocytomas (PEM), 4 deep-penetrating nevi, and 1 atypical cellular blue nevus. This study adds to the growing body of knowledge on pediatric atypical cutaneous melanocytic proliferations, aligning with many described characteristics such as disease location and overall survival rates, with distinct exceptions (higher melanoma positive SLNB rate, lower atypical Spitz tumor WLE rate, and a case of fatal metastatic atypical Spitz tumor).
The Importance of Optimal Thermal Ablation Margins in Colorectal Liver Metastases: A Systematic Review and Meta-Analysis of 21 Studies
Authors: Chlorogiannis David-Dimitris , Sotirchos Vlasios S. , Georgiades Christos , Filippiadis Dimitrios , Arellano Ronald S. , Gonen Mithat , Makris Gregory C. , Garg Tushar , Sofocleous Constantinos T.
Published: December 2023

Background: Colorectal cancer (CRC) is the second most common cause of cancer-related deaths in the US. Thermal ablation (TA) can be a comparable alternative to partial hepatectomy for selected cases when eradication of all visible tumor with an ablative margin of greater than 5 mm is achieved. This systematic review and meta-analysis aimed to encapsulate the current clinical evidence concerning the optimal TA margin for local cure in patients with colorectal liver metastases (CLM). Methods: MEDLINE, EMBASE, and the CENTRAL databases were systematically searched from inception until 1 May 2023, in accordance with the PRISMA Guidelines. Measure of effect included the risk ratio (RR) with 95% confidence interval (CI) using the random-effects model. Results: Overall, 21 studies were included, comprising 2005 participants and 2873 ablated CLMs. TA with margins less than 5 mm were associated with a 3.6 times higher risk for LTP (n = 21 studies, RR: 3.60; 95% CI: 2.58–5.03; p-value < 0.001). When margins less than 5 mm were additionally confirmed by using 3D software, a 5.1 times higher risk for LTP (n = 4 studies, RR: 5.10; 95% CI: 1.45–17.90; p-value < 0.001) was recorded. Moreover, a thermal ablation margin of less than 10 mm but over 5 mm remained significantly associated with 3.64 times higher risk for LTP vs. minimal margin larger than 10 mm (n = 7 studies, RR: 3.64; 95% CI: 1.31–10.10; p-value < 0.001). Conclusions: This meta-analysis solidifies that a minimal ablation margin over 5 mm is the minimum critical endpoint required, whereas a minimal margin of at least 10 mm yields optimal local tumor control after TA of CLMs.
Factors Associated with Poor Quality of Life in Breast Cancer Survivors: A 3-Year Follow-Up Study
Authors: Kim Soo-Hyun , Jo Ha-Yeon
Published: December 2023

The purpose of this study was to identify subgroups of quality of life (QOL) changes in breast cancer survivors (BCSs), and to determine factors associated with subgroups of consistently low or deteriorated QOL. We enrolled 101 women recently diagnosed with breast cancer in South Korea and asked them to complete a questionnaire at baseline (within 1 month of diagnosis), 1 year later (Year 1), 2 years later (Year 2), and 3 years later (Year 3). We assessed QOL using the global QOL subscale from the EORTC QLQ-C30. We defined low QOL as a global QOL score 10 points below the mean score of the general population. Based on low QOL as defined in this study, we identified subgroups of QOL changes over 3 years. We identified four subgroups of QOL changes: improved (47.4%), stable (30%), continuously low (8.8%), and deteriorated (13.8%), and considered the last two categories (22.6%) poor QOL. Logistic regression analyses demonstrated that significant determinants of poor QOL were insomnia at Year 1, fatigue and anxiety at Year 2, and fatigue, depression, and comorbidity at Year 3. In conclusion, persistent symptoms of insomnia, fatigue, anxiety, depression, and comorbidity are potential risk factors for poor QOL in BCSs.
Experimental Modeling of Host–Bacterial Interactions in Head and Neck Squamous Cell Carcinoma
Authors: Okolo Ogoegbunam , Honzel Emily , Britton William R. , Yu Victoria X. , Flashner Samuel , Martin Cecilia , Nakagawa Hiroshi , Parikh Anuraag S.
Published: December 2023

The microscopic species colonizing the human body, collectively referred to as the microbiome, play a crucial role in the maintenance of tissue homeostasis, immunity, and the development of disease. There is evidence to suggest associations between alterations in the microbiome and the development of head and neck squamous cell carcinomas (HNSCC). The use of two-dimensional (2D) modeling systems has made significant strides in uncovering the role of microbes in carcinogenesis; however, direct mechanistic links remain in their infancy. Patient-derived three-dimensional (3D) HNSCC organoid and organotypic models have recently been described. Compared to 2D models, 3D organoid culture systems effectively capture the genetic and epigenetic features of parent tissue in a patient-specific manner and may offer a more nuanced understanding of the role of host–microbe responses in carcinogenesis. This review provides a topical literature review assessing the current state of the field investigating the role of the microbiome in HNSCC; including in vivo and in vitro modeling methods that may be used to characterize microbiome–epithelial interactions.
Five Fractions versus Seven Fractions SBRT for Intermediate- and High-Risk Prostate Cancer: A Propensity Score Matched Pair Analysis
Authors: Schröder Christina , Mose Lucas , Mathier Etienne , Zwahlen Daniel Rudolf , Aebersold Daniel Matthias , Förster Robert , Shelan Mohamed
Published: December 2023

Purpose: To compare two stereotactic body radiotherapy (SBRT) regimens in patients with intermediate- or high-risk prostate cancer with regards toxicity and efficacy. Methods/Material: We retrospectively collected data from 198 patients treated with SBRT for prostate cancer at two different institutions. Patients received either 35–36.25 Gy in five fractions (group A) using Cyberknife robotic platform or 42.7 Gy in seven fractions (group B) using a C-arm LINAC (image-guided). Propensity score matching was done (2:1 nearest neighbor matching without replacement), resulting in 120 patients (80 patients for group A, 40 patients for group B). Toxicity, PSA nadir, biochemical failure and disease-free survival (DFS) were analyzed. Results: Median follow up of all patients was 13 months (range 1–91 months). Overall, 23.3% of patients had ≥G2 acute GU toxicity (21.1% group A versus 30% group B (p = 0.222)) and 6.6% of patients ≥G2 GI toxicity (2.5% versus 15% (p = 0.010)). There was one acute G3 GU toxicity in arm A and one acute G4 rectal bleeding in group B (anticoagulated patient). Regarding late toxicity, 14.1% of patients had ≥G2 late GU toxicity (17.4% versus 6.6% (p = 0.159)) and 5.0% of patients had ≥G2 late GI toxicity (1.4% versus 13.3% (p = 0.013)). There was one G3 late GU toxicity in arm B and two G3 late GI toxicities, one in each arm. Relative median PSA reduction was 92.4% (−53.9–99.9%) from baseline PSA (93.7% (−53.9–99.9%) in group A versus 87.7% (39.8–99.9%) in group B (p = 0.043). In total, 4.2% of patients had biochemical relapse, 5.0% in group A and 2.5% in group B (p = 0.518). One-year DFS in the overall cohort was 97.3%, 98.8% in group A and 94.3% in group B (p = 0.318). Conclusion: Both SBRT regimens have acceptable acute and late toxicity and good efficacy. There are significantly more GI toxicities in the seven-fraction regimen. Longer follow-up is warranted for better comparison of long-term efficacy.
Radiogenomics: Contemporary Applications in the Management of Rectal Cancer
Authors: O’Sullivan Niall J. , Temperley Hugo C. , Horan Michelle T. , Corr Alison , Mehigan Brian J. , Larkin John O. , McCormick Paul H. , Kavanagh Dara O. , Meaney James F. M. , Kelly Michael E.
Published: December 2023

Radiogenomics, a sub-domain of radiomics, refers to the prediction of underlying tumour biology using non-invasive imaging markers. This novel technology intends to reduce the high costs, workload and invasiveness associated with traditional genetic testing via the development of ‘imaging biomarkers’ that have the potential to serve as an alternative ‘liquid-biopsy’ in the determination of tumour biological characteristics. Radiogenomics also harnesses the potential to unlock aspects of tumour biology which are not possible to assess by conventional biopsy-based methods, such as full tumour burden, intra-/inter-lesion heterogeneity and the possibility of providing the information of tumour biology longitudinally. Several studies have shown the feasibility of developing a radiogenomic-based signature to predict treatment outcomes and tumour characteristics; however, many lack prospective, external validation. We performed a systematic review of the current literature surrounding the use of radiogenomics in rectal cancer to predict underlying tumour biology.
Mice Overexpressing Wild-Type RRAS2 Are a Novel Model for Preclinical Testing of Anti-Chronic Lymphocytic Leukemia Therapies
Authors: Hortal Alejandro M. , Villanueva Ana , Arellano Irene , Prieto Cristina , Mendoza Pilar , Bustelo Xosé R. , Alarcón Balbino
Published: December 2023

B-cell chronic lymphocytic leukemia (B-CLL) is the most common type of leukemia in the Western world. Mutation in different genes, such as TP53 and ATM, and deletions at specific chromosomic regions, among which are 11q or 17p, have been described to be associated to worse disease prognosis. Recent research from our group has demonstrated that, contrary to what is the usual cancer development process through missense mutations, B-CLL is driven by the overexpression of the small GTPase RRAS2 in its wild-type form without activating mutations. Some mouse models of this disease have been developed to date and are commonly used in B-CLL research, but they present different disadvantages such as the long waiting period until the leukemia fully develops, the need to do cell engraftment or, in some cases, the fact that the model does not recapitulate the alterations found in human patients. We have recently described Rosa26-RRAS2fl/flxmb1-Cre as a new mouse model of B-CLL with a full penetrance of the disease. In this work, we have validated this mouse model as a novel tool for the development of new therapies for B-CLL, by testing two of the most broadly applied targeted agents: ibrutinib and venetoclax. This also opens the door to new targeted agents against R-RAS2 itself, an approach not yet explored in the clinic.
The Role of Alcohols in the Hexene-1 Hydroalkoxycarbonylation Reaction with Catalysts Based on Palladium Complexes
Authors: Zhaksylykova Gulbanu , Shalmagambetov Kairzhan , Kanapiyeva Fatima , Kudaibergenov Nurbolat , Bulybayev Marat , Zykai Meruyert , Abyzbekova Gulmira , Balykbayeva Gulzhan
Published: December 2023

In this work, the activity of various alcohols in the hexene-1 hydroalkoxycarbonylation reaction in the presence of two catalytic systems was investigated for the first time: (1) Pd(PPh3)4-PPh3-TsOH (menthol, cyclohexanol, ethanol, propanol, iso-propanol, butanol, isobutanol and benzyl alcohol) and (2) PdCl2(PPh3)2-PPh3-AlCl3 (ethanol, propanol-1, butanol-1, isoamyl alcohol, isobutanol, pentanol-1, allyl alcohol and tert-butyl alcohol). The optimal process parameters (temperature, pressure and reaction time) for the reactions of the hydropropoxycarbonylation and hydrobutoxycarbonylation of hexene-1, at which the yields of target products reached 91.8% and 91.5%, respectively, were determined.
Age-Related Alternative Splicing: Driver or Passenger in the Aging Process?
Authors: Baralle Marco , Romano Maurizio
Published: December 2023

Alternative splicing changes are closely linked to aging, though it remains unclear if they are drivers or effects. As organisms age, splicing patterns change, varying gene isoform levels and functions. These changes may contribute to aging alterations rather than just reflect declining RNA quality control. Three main splicing types—intron retention, cassette exons, and cryptic exons—play key roles in age-related complexity. These events modify protein domains and increase nonsense-mediated decay, shifting protein isoform levels and functions. This may potentially drive aging or serve as a biomarker. Fluctuations in splicing factor expression also occur with aging. Somatic mutations in splicing genes can also promote aging and age-related disease. The interplay between splicing and aging has major implications for aging biology, though differentiating correlation and causation remains challenging. Declaring a splicing factor or event as a driver requires comprehensive evaluation of the associated molecular and physiological changes. A greater understanding of how RNA splicing machinery and downstream targets are impacted by aging is essential to conclusively establish the role of splicing in driving aging, representing a promising area with key implications for understanding aging, developing novel therapeutical options, and ultimately leading to an increase in the healthy human lifespan.
The Liaison between Metabolism and Oxidative Stress in Human Diseases
Authors: Ravera Silvia , Panfoli Isabella
Published: December 2023

Mitochondria have been the prerequisite to eukaryote complexity since their likely endosymbiotic origin, allowing a remarkable expansion in the number of genes expressed [...]
Microglial Senescence and Activation in Healthy Aging and Alzheimer’s Disease: Systematic Review and Neuropathological Scoring
Authors: Malvaso Antonio , Gatti Alberto , Negro Giulia , Calatozzolo Chiara , Medici Valentina , Poloni Tino Emanuele
Published: December 2023

The greatest risk factor for neurodegeneration is the aging of the multiple cell types of human CNS, among which microglia are important because they are the “sentinels” of internal and external perturbations and have long lifespans. We aim to emphasize microglial signatures in physiologic brain aging and Alzheimer’s disease (AD). A systematic literature search of all published articles about microglial senescence in human healthy aging and AD was performed, searching for PubMed and Scopus online databases. Among 1947 articles screened, a total of 289 articles were assessed for full-text eligibility. Microglial transcriptomic, phenotypic, and neuropathological profiles were analyzed comprising healthy aging and AD. Our review highlights that studies on animal models only partially clarify what happens in humans. Human and mice microglia are hugely heterogeneous. Like a two-sided coin, microglia can be protective or harmful, depending on the context. Brain health depends upon a balance between the actions and reactions of microglia maintaining brain homeostasis in cooperation with other cell types (especially astrocytes and oligodendrocytes). During aging, accumulating oxidative stress and mitochondrial dysfunction weaken microglia leading to dystrophic/senescent, otherwise over-reactive, phenotype-enhancing neurodegenerative phenomena. Microglia are crucial for managing Aβ, pTAU, and damaged synapses, being pivotal in AD pathogenesis.