bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Study of the Blood–Brain Barrier Transport of Natural Compounds Recovered from Agrifood By-Products and Microalgae
Authors: Sánchez-Martínez José David , Garcia Ana Rita , Alvarez-Rivera Gerardo , Valdés Alberto , Brito Maria Alexandra , Cifuentes Alejandro
Published: December 2022

Agrifood by-products and microalgae represent a low-cost and valuable source of bioactive compounds with neuroprotective properties. However, the neuroprotective effectiveness of therapeutic molecules can be limited by their capacity to cross the blood–brain barrier (BBB) and reach the brain. In this research, various green extracts from Robinia pseudoacacia (ASFE), Cyphomandra betacea (T33), Coffea arabica (PPC1), Olea europaea L., (OL-SS), Citrus sinensis (PLE100) by-products and from the microalgae Dunaliella salina (DS) that have demonstrated in vitro neuroprotective potential were submitted to an in vitro BBB permeability and transport assay based on an immortalized human brain microvascular endothelial cells (HBMEC) model. Toxicity and BBB integrity tests were performed, and the transport of target bioactive molecules across the BBB were evaluated after 2 and 4 h of incubation using gas and liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (GC/LC-Q-TOF-MS). The HBMEC-BBB transport assay revealed a high permeability of representative neuroprotective compounds, such as mono- and sesquiterpenoids, phytosterols and some phenolic compounds. The obtained results from the proposed in vitro BBB cellular model provide further evidence of the neuroprotective potential of the target natural extracts, which represent a promising source of functional ingredients to be transferred into food supplements, food additives, or nutraceuticals with scientifically supported neuroprotective claims.
Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta
Authors: Trifunović Svetlana , Šošić Jurjević Branka , Ristić Nataša , Nestorović Nataša , Filipović Branko , Stevanović Ivana , Begović-Kuprešanin Vesna , Manojlović-Stojanoski Milica
Published: December 2022

As the mediator between the mother and fetus, the placenta allows the most appropriate environment and optimal fetal growth. The placenta of one sex sometimes has a greater ability over the other to respond to and protect against possible maternal insults. Here, we characterized sex differences in the placenta’s morphological features and antioxidant status following dexamethasone (Dx) exposure. Pregnant rats were exposed to Dx or saline. The placenta was histologically and stereologically analyzed. The activity of the antioxidant enzymes, lipid peroxides (TBARS), superoxide anion and nitric oxide (NO) was measured. The decrease in placental zone volumes was more pronounced (p < 0.05) in female placentas. The volume density of PCNA-immunopositive nuclei was reduced (p < 0.05) in both sexes. The reduced (p < 0.05) antioxidant enzyme activities, enhanced TBARS and NO concentration indicate that Dx exposure triggered oxidative stress in the placenta of both fetal sexes, albeit stronger in the placenta of female fetuses. In conclusion, maternal Dx treatment reduced the size and volume of placental zones, altered placental histomorphology, decreased cell proliferation and triggered oxidative stress; however, the placentas of female fetuses exerted more significant responses to the treatment effects. The reduced placental size most probably reduced the transport of nutrients and oxygen, thus resulting in the reduced weight of fetuses, similar in both sexes. The lesser ability of the male placenta to detect and react to maternal exposure to environmental challenges may lead to long-standing health effects.
Follow-Up Biomarkers in the Evolution of Prostate Cancer, Levels of S100A4 as a Detector in Plasma
Authors: Alvarez-Cubero Maria Jesus , Arance Elena , de Santiago Esperanza , Sanchez Pilar , Sepúlveda Maria Rosario , Marrero Raquel , Lorente Jose Antonio , Gonzalez-Cabezuelo Jose Maria , Cuenca-Lopez Sergio , Cozar Jose Manuel , Vazquez-Alonso Fernando , Martinez-Gonzalez Luis Javier
Published: December 2022

The management and screening of prostate cancer (PC) is still the main problem in clinical practice. In this study, we investigated the role of aggressiveness genetic markers for PC stratification. We analyzed 201 plasma samples from PC patients and controls by digital PCR. For selection and validation, 26 formalin-fixed paraffin-embedded tissues, 12 fresh tissues, and 24 plasma samples were characterized by RNA-Seq, immunochemistry, immunofluorescence, Western blot, and extracellular-vesicles analyses. We identified three novel non-invasive biomarkers; all with an increased expression pattern in patients (PCA3: p = 0.002, S100A4: p ≤ 0.0001 and MRC2: p = 0.005). S100A4 presents the most informative AUC (area under the curve) (0.735). Combination of S100A4, MRC2, and PCA3 increases the discriminatory power between patients and controls and between different more and less aggressive stages (AUC = 0.761, p ≤ 0.0001). However, although a sensitivity of 97.47% in PCA3 and a specificity of 90.32% in S100A4 was reached, the detection signal level could be variable in some analyses owing to tumor heterogeneity. This is the first time that the role of S100A4 and MRC2 has been described in PC aggressiveness. Moreover, the combination of S100A4, MRC2, and PCA3 has never been described as a non-invasive biomarker for PC screening and aggressiveness.
Structure and Function of the Zinc Binding Protein ZrgA from Vibrio cholerae
Authors: Valencia Daniel W. , Meléndez Ady B. , Melendrez Isaac A. , Yukl Erik T.
Published: December 2022

ATP binding cassette (ABC) transporters are the primary means by which bacteria acquire trace elements from the environment. They rely on solute binding proteins (SBPs) to bind the relevant substrate and deliver it to the integral membrane permease for ATP-powered import into the cytoplasm. SBPs of cluster A-I are known to facilitate the transport of essential metals zinc, manganese, and iron, and many have been characterized to date. A group of ABC transporter operons dubbed zinc-regulated genes (zrg) have recently been shown to transport zinc with putative SBPs (zrgA) bearing no homology to the classical cluster A-I family, and a recent crystal structure of a representative protein from Pseudomonas aeruginosa shows no structural similarity to classical SBPs. Thus, the ZrgA proteins appear to represent a newly discovered family of zinc SBPs widespread among Gram-negative bacteria, including human pathogens. Here, we have determined the crystal structure of ZrgA from Vibrio cholerae and characterized its zinc binding in vitro and function in vivo. We also assessed the role of a histidine-rich sequence that appears to be a hallmark of ZrgA proteins that is particularly long in V. cholerae ZrgA. The results show that the zrgA gene is critical to the function of the operon, consistent with a function as an SBP in this system. Further, the His-rich region is not essential to the function of ZrgA, but it does provide additional zinc binding sites in vitro. The structure and zinc binding data for ZrgA reveal interesting differences between it and its homologue from P. aeruginosa, illustrating diversity within this little-studied protein family.
It Runs in the Bromodomain Family: Speckled Proteins (SP) Play a Role in the Antitumor Immune Response in Solid Tumors
Authors: Rosochowicz Monika Anna , Lipowicz Julia Maria , Karwacka Marianna Iga , Ostapowicz Julia , Cisek Malgorzata , Mackiewicz Andrzej Adam , Czerwinska Patrycja
Published: December 2022

Cells and immune cells in the extracellular matrix: Depending on the tumor type and variety of TAAs (tumor-associated antigens), immune infiltrates are composed of many different subpopulations of immune cells. Epigenetic changes are also considered to be characteristic of cancer. Epigenetic factors taking part in the regulation of gene expression include the VII group of bromodomain proteins (BrD)—SP-family proteins. Here, we used transcriptomic data from the TCGA database, as well as immunological evidence from ESTIMATE, TIP, and TIMER2.0 databases for various solid tumor types and harnessed several publicly available bioinformatic tools (such as GSEA and GSCA) to demonstrate mechanisms and interactions between BrD proteins and immune infiltrates in cancer. We present a consistently positive correlation between the SP-family genes and immune score regardless of the tumor type. The SP-family proteins correlate positively with T cells’ trafficking and infiltration into tumor. Our results also show an association between the high expression of SP family genes and enriched transcriptome profiles of inflammatory response and TNF-α signaling via NF-κβ. We also show that the SP-family proteins could be considered good predictors of high immune infiltration phenotypes.
Genome-Wide Characterization and Comparative Genomic Analysis of the Serpin Gene Family in Microsporidian Nosema bombycis
Authors: Ran Maoshuang , Shi Yulian , Li Boning , Xiang Heng , Tao Meilin , Meng Xianzhi , Li Tian , Li Chunfeng , Bao Jialing , Pan Guoqing , Zhou Zeyang
Published: December 2022

Microsporidia are ubiquitous in the environment, infecting almost all invertebrates, vertebrates, and some protists. The microsporidian Nosema bombycis causes silkworms pébrine disease and leads to huge economic losses. Parasite secreted proteins play vital roles in pathogen–host interactions. Serine protease inhibitors (serpins), belonging to the largest and most broadly distributed protease inhibitor superfamily, are also found in Microsporidia. In this study, we characterized 19 serpins (NbSPNs) in N. bombycis; eight of them were predicted with signal peptides. All NbSPN proteins contain a typical conserved serpin (PF00079) domain. The comparative genomic analysis revealed that microsporidia serpins were only found in the genus Nosema. In addition to N. bombycis, a total of 34 serpins were identified in another six species of Nosema including N. antheraeae (11), N. granulosis (8), Nosema sp. YNPr (3), Nosema sp. PM-1 (3), N. apis (4), and N. ceranae (5). Serpin gene duplications in tandem obviously occurred in Nosema antheranae. Notably, the NbSPNs were phylogenetically clustered with serpins from the Chordopoxvirinae, the subfamily of Poxvirus. All 19 NbSPN transcripts were detected in the infected midgut and fat body, while 19 NbSPN genes except for NbSPN12 were found in the transcriptome of the infected silkworm embryonic cell line BmE-SWU1. Our work paves the way for further study of serpin function in microsporidia.
Analysis of Enzyme Activity and Cellular Function for the N80S and S480F Asparagine Synthetase Variants Expressed in a Child with Asparagine Synthetase Deficiency
Authors: Staklinski Stephen J. , Snanoudj Sarah , Guerrot Anne-Marie , Vanhulle Catherine , Lecoquierre François , Bekri Soumeya , Kilberg Michael S.
Published: December 2022

Asparagine Synthetase Deficiency (ASNSD) is a disease caused by mutations in asparagine synthetase (ASNS). Newborns exhibit microcephaly, intractable epileptic-like seizures, progressive brain atrophy, and axial hypotonia. ASNSD results in global developmental delays and premature death. The present report describes a 9-year-old child who is a compound heterozygote with ASNS mutations c.1439C > T and c.239A > G leading to variants p.S480F and p.N80S, respectively. When grown in a complete culture medium, primary fibroblasts from the child contained ASNS mRNA and protein levels similar to an unrelated wild-type fibroblast cell line. When the child’s fibroblasts were cultured for up to 72 h in a medium lacking asparagine, proliferation was reduced by about 50%. Purification of ASNS proteins harboring either the S480F or the N80S substitution had reduced enzymatic activity by 80% and 50%, respectively. Ectopic expression of either variant in ASNS-null Jensen rat sarcoma (JRS) cells did not support proliferation in the absence of medium-supplied asparagine, whereas expression of wild-type enzyme completely restored growth. These studies add to the list of pathogenic ASNS variants and use enzyme activity and protein expression in ASNS-null cells to expand our knowledge of the biological impact of mutations in the ASNS gene.
Allopregnanolone: Metabolism, Mechanisms of Action, and Its Role in Cancer
Authors: Zamora-Sánchez Carmen J. , Camacho-Arroyo Ignacio
Published: December 2022

Allopregnanolone (3α-THP) has been one of the most studied progesterone metabolites for decades. 3α-THP and its synthetic analogs have been evaluated as therapeutic agents for pathologies such as anxiety and depression. Enzymes involved in the metabolism of 3α-THP are expressed in classical and nonclassical steroidogenic tissues. Additionally, due to its chemical structure, 3α-THP presents high affinity and agonist activity for nuclear and membrane receptors of neuroactive steroids and neurotransmitters, such as the Pregnane X Receptor (PXR), membrane progesterone receptors (mPR) and the ionotropic GABAA receptor, among others. 3α-THP has immunomodulator and antiapoptotic properties. It also induces cell proliferation and migration, all of which are critical processes involved in cancer progression. Recently the study of 3α-THP has indicated that low physiological concentrations of this metabolite induce the progression of several types of cancer, such as breast, ovarian, and glioblastoma, while high concentrations inhibit it. In this review, we explore current knowledge on the metabolism and mechanisms of action of 3α-THP in normal and tumor cells.
Bone Marrow Microenvironment as a Source of New Drug Targets for the Treatment of Acute Myeloid Leukaemia
Authors: Skelding Kathryn A. , Barry Daniel L. , Theron Danielle Z. , Lincz Lisa F.
Published: December 2022

Acute myeloid leukaemia (AML) is a heterogeneous disease with one of the worst survival rates of all cancers. The bone marrow microenvironment is increasingly being recognised as an important mediator of AML chemoresistance and relapse, supporting leukaemia stem cell survival through interactions among stromal, haematopoietic progenitor and leukaemic cells. Traditional therapies targeting leukaemic cells have failed to improve long term survival rates, and as such, the bone marrow niche has become a promising new source of potential therapeutic targets, particularly for relapsed and refractory AML. This review briefly discusses the role of the bone marrow microenvironment in AML development and progression, and as a source of novel therapeutic targets for AML. The main focus of this review is on drugs that modulate/target this bone marrow microenvironment and have been examined in in vivo models or clinically.
Sensitive and Selective Voltammetric Sensor Based on Anionic Surfactant-Modified Screen-Printed Carbon for the Quantitative Analysis of an Anticancer Active Fused Azaisocytosine-Containing Congener
Authors: Kozak Jędrzej , Tyszczuk-Rotko Katarzyna , Sztanke Krzysztof , Sztanke Małgorzata
Published: December 2022

3-(4-Nitrophenyl)-8-(2,3-dimethylphenyl)-7,8-dihydroimidazo[2,1-c][1,2,4]triazin-4(6H)-one (NDIT) is one of the most promising candidates for anticancer agents. Hence, a sensitive and selective sodium dodecyl sulfate-modified screen-printed carbon sensor (SPCE/SDS) was used for its quantitative analysis. The SPCE/SDS, in contrast to the SPCE, showed excellent behavior in the electrochemical reduction of NDIT by differential-pulse adsorptive stripping voltammetry (DPAdSV). Cyclic voltammetric (CV) studies reveal an irreversible, two-stage and not purely diffusion-controlled reduction process in 0.01 M HNO3. The sensor was characterized by CV and electrochemical impedance spectroscopy (EIS). Under the optimized conditions (t 45 s, ΔE 175 mV, ν 150 mV/s, and tm 5 ms), the DPAdSV procedure with the SPCE/SDS presented a very wide linear range from 1 to 2000 nM and a low detection limit of 0.29 nM. A 1000-fold excess concentration of potential interferents commonly present in biological samples did not significantly alter the peak current of NDIT. The practical application of the proposed DPAdSV procedure with the SPCE/SDS was successfully checked by analyzing spiked human serum samples.
Ghrelin Alleviates Experimental Ulcerative Colitis in Old Mice and Modulates Colonocyte Metabolism via PPARγ Pathway
Authors: Muthyala Srilakshmi , Chapkin Robert S. , Wu Chaodong , Wu Chia-Shan
Published: December 2022

There is a growing prevalence of inflammatory bowel disease (IBD), a chronic inflammatory condition of the gastrointestinal tract, among the aging population. Ghrelin is a gut hormone that, in addition to controlling feeding and energy metabolism, has been shown to exert anti-inflammatory effects; however, the effect of ghrelin in protecting against colitis in old mice has not been assessed. Here, we subjected old female C57BL/6J mice to dextran sulfate sodium (DSS) in drinking water for six days, then switched back to normal drinking water, administered acyl-ghrelin or vehicle control from day 3 to 13, and monitored disease activities throughout the disease course. Our results showed that treatment of old mice with acyl-ghrelin attenuated DSS-induced colitis. Compared to the DSS group, ghrelin treatment decreased levels of the inflammation marker S100A9 in the colons collected on day 14 but not on day 8, suggesting that the anti-inflammatory effect was more prominent in the recovery phase. Ghrelin treatment also significantly reduced F4/80 and interleukin-17A on day 14. Moreover, acyl-ghrelin increased mitochondrial respiration and activated transcriptional activity of the peroxisome proliferator-activated receptor gamma (PPARγ) in Caco-2 cells. Together, our data show that ghrelin alleviated DSS-induced colitis, suggesting that ghrelin may promote tissue repair in part through regulating epithelial metabolism via PPARγ mediated signaling.
Relationships between Diabetes and the Intestinal Microbial Population
Authors: Bondy Stephen C.
Published: December 2022

Diabetes is a metabolic disorder characterized by lower responsiveness of tissues to insulin and consequent large variations in circulating levels of glucose. This fluctuation has harmful effects as both hyperglycemia and hypoglycemia can be very injurious. The causes of diabetes are varied but the consequences are rather uniform. Dietary factors are important especially in adult onset type 2 diabetes (T2D) while type 1 diabetes (T1D) is characterized by having a stronger heritable component and involving autoimmune attach on pancreatic beta cells. This review is focused on the relation of the bacterial components found within the intestine, to the establishment and maintenance of diabetes. The precise composition of the gut microbiome is increasingly recognized as a factor in organismic health and its interaction with a variety of disease states has been described. This is especially marked in the case of diabetes since the nature of the diet is an important factor in establishing both the microbiome and the incidence of diabetes. The bidirectional nature of this relationship is discussed. The effects of disease that lead to altered microbiomal composition together with aberrant metabolic changes are also included. Emphasis is given to the important role of short chain fatty acids (SCFAs) as mediators of the microbiome-diabetes relation.
The Optimization of Renal Graft Preservation Temperature to Mitigate Cold Ischemia-Reperfusion Injury in Kidney Transplantation
Authors: Abou Taka Maria , Dugbartey George J. , Sener Alp
Published: December 2022

Renal transplantation is the preferred treatment for patients with end-stage renal disease. The current gold standard of kidney preservation for transplantation is static cold storage (SCS) at 4 °C. However, SCS contributes to renal ischemia-reperfusion injury (IRI), a pathological process that negatively impacts graft survival and function. Recent efforts to mitigate cold renal IRI involve preserving renal grafts at higher or subnormothermic temperatures. These temperatures may be beneficial in reducing the risk of cold renal IRI, while also maintaining active biological processes such as increasing the expression of mitochondrial protective metabolites. In this review, we discuss different preservation temperatures for renal transplantation and pharmacological supplementation of kidney preservation solutions with hydrogen sulfide to determine an optimal preservation temperature to mitigate cold renal IRI and enhance renal graft function and recipient survival.
Instantaneous Frequency Estimation of FM Signals under Gaussian and Symmetric α-Stable Noise: Deep Learning versus Time–Frequency Analysis
Authors: Razzaq Huda Saleem , Hussain Zahir M.
Published: December 2022

Deep learning (DL) and machine learning (ML) are widely used in many fields but rarely used in the frequency estimation (FE) and slope estimation (SE) of signals. Frequency and slope estimation for frequency-modulated (FM) and single-tone sinusoidal signals are essential in various applications, such as wireless communications, sound navigation and ranging (SONAR), and radio detection and ranging (RADAR) measurements. This work proposed a novel frequency estimation technique for instantaneous linear FM (LFM) sinusoidal wave using deep learning. Deep neural networks (DNN) and convolutional neural networks (CNN) are classes of artificial neural networks (ANNs) used for the frequency and slope estimation for LFM signals under additive white Gaussian noise (AWGN) and additive symmetric alpha stable noise (SαSN). DNN is composed of input, output, and two hidden layers, where several nodes in the first and second hidden layers are 25 and 8, respectively. CNN is the content input layer; many hidden layers include convolution, batch normalization, ReLU, max pooling, fully connected, and dropout. The output layer consists of a fully connected softmax and classification layers. SαS distributions are impulsive noise disturbances found in many communication environments such as marine systems, their distribution lacks a closed-form probability density function (PDF), except for specific cases, and infinite second-order statistics, hence geometric SNR (GSNR) is used in this work to determine the effect of noise in a mixture of Gaussian and SαS noise processes. DNN is a machine learning classifier with few layers for reducing FE and SE complexity. CNN is a deep learning classifier, designed with many layers, and proved to be more accurate than DNN when dealing with big data and finding optimal features. Simulation results show that SαS noise can be much more harmful to the FE and SE of FM signals than Gaussian noise. DL and ML can significantly reduce FE complexity, memory cost, and power consumption as compared to the classical FE based on time–frequency analysis, which are important requirements for many systems, such as some Internet of Things (IoT) sensor applications. After training CNN for frequency and slope estimation of LFM signals, the performance of CNN (in terms of accuracy) can give good results at very low signal-to-noise ratios where time–frequency distribution (TFD) fails, giving more than 20 dB difference in the GSNR working range as compared to the classical spectrogram-based estimation, and over 15 dB difference with Viterbi-based estimate.
Landscape and Predictive Significance of the Structural Classification of EGFR Mutations in Chinese NSCLCs: A Real-World Study
Authors: Gu Linping , Huang Huayan , Xu Zhangwendi , Niu Xiaomin , Li Ziming , Xia Liliang , Yu Yongfeng , Lu Shun
Published: December 2022

Background: Non-classical EGFR mutations demonstrate heterogeneous and attenuated responsiveness to EGFR TKIs. Non-small cell lung cancer (NSCLC) patients with atypical EGFR mutations have limited therapeutic options. A recent study established a novel structural-based classification of EGFR mutations and showed its value in predicting the response to TKI. We sought to interrogate the distribution of different structural types and to validate the predictive value in Chinese NSCLCs. Methods: A total of 837 tumor samples were retrospectively recruited from 522 patients with unresectable EGFR-mutant NSCLC. EGFR mutations were classified into four groups: classical-like, T790M-like, Ex20ins-L, and PACC. Treatment information and clinical outcomes were obtained from 436 patients. The time to treatment failure (TTF) was determined on a per-sample basis. Results: Of the 837 EGFR-mutant samples, 67.9%, 18.5%, 9.0%, and 3.1% harbored classical-like, T790M-like, PACC, and Ex20ins-L mutations, respectively. Thirteen (1.6%) samples carried mutations beyond the four types. Among the 204 samples with atypical mutations, 33.8%, 36.7%, 12.7%, and 10.3% were classical-like, PACC, Ex20ins-L, and T790M-like, respectively. In patients with PACC mutations, second-generation TKIs demonstrated a significantly longer TTF than first-generation TKIs (first-line: 15.3 vs. 6.2 months, p = 0.009; all-line: 14.7 vs. 7.1 months, p = 0.003), and a trend of longer TTF than third-generation TKIs (all-line: 14.7 vs. 5.1 months, p = 0.135). Conclusions: Our study depicted the landscape of structural types of EGFR mutations in Chinese NSCLC patients. Our results also suggest that the structural classification can serve as a predictive marker for the efficacy of various EGFR TKIs, which would guide therapeutic decision making.
Selective Decontamination of the Digestive Tract in Pancreatic Head Resections—A Propensity Score-Matched Analysis
Authors: Radulova-Mauersberger Olga , Oehme Florian , Doerell Alexandra , Frohneberg Laura , Hempel Sebastian , Weitz Jürgen , Welsch Thilo , Distler Marius , Teske Christian
Published: December 2022

(1) Background: The postoperative morbidity rate after pancreatic head resection remains high, partly due to infectious complications. The primary aim of this study was to analyze the influence of selective decontamination of the digestive tract (SDD) on the postoperative infection rate after pancreatic surgery. (2) Methods: From January 2019, the standard of care for patients undergoing pancreatic head resections at the Department for Visceral, Thoracic, and Vascular Surgery, University Hospital Dresden was the preoperative oral administration of SDD. The influence of SDD was evaluated for patients operated on between January 2019 and June 2020 in comparison to a propensity score-matched cohort, extracted from an existing database including all pancreatic resections from 2012 to 2018. The primary endpoint of the study was the shift of the bacterial load on the intraoperative bile swab test. The secondary endpoint was the association of SDD with postoperative complications. (3) Results: In total, 200 patients either with SDD (n = 100; 50%) or without SDD (non-SDD, n = 100; 50%) were analyzed. In the patient group without a preoperative biliary stent, 44% (n = 11) of the non-SDD group displayed positive bacterial results, whereas that was the case for only 21.7% (n = 10) in the SDD group (p = 0.05). Particularly, Enterobacter species (spp.) were reduced from 41.2% (n = 14) (non-SDD group) to 23.5% (n = 12) (SDD group) (p = 0.08), and Citrobacter spp. were reduced by 13.7% (p = 0.09) from the non-SDD to the SDD cohort. In patients with a preoperative biliary stent, the Gram-negative Enterobacter spp. were significantly reduced from 52.2% (n = 12) in the non-SDD group to 26.8% (n = 11) in the SDD group (p = 0.04). Similarly, Citrobacter spp. decreased by 20.6% from 30.4% (n = 7) to 9.8% (n = 4) in the non-SDD compared to the SDD group (p = 0.04). In general, deep fluid collection and abscesses occurred more frequently in the non-SDD group (36%; n = 36 vs. 27%; n = 27; p = 0.17). (4) Conclusions: Adoption of SDD before pancreatic head surgery may reduce the bacterial load in bile fluid. SDD administration does not significantly affect the postoperative infectious complication rate after pancreatic head resections.
Phosphaturic Mesenchymal Tumors: Rethinking the Clinical Diagnosis and Surgical Treatment
Authors: Liu Yupeng , He Hongbo , Zhang Can , Zeng Hao , Tong Xiaopeng , Liu Qing
Published: December 2022

Background: The diagnosis of phosphaturic mesenchymal tumors (PMT) is easily delayed clinically, and their surgical treatment is unstandardized. This study aimed to evaluate our experience in the diagnosis and treatment of PMT and provide a research basis for the accurate and standardized treatment of PMT. Materials and Methods: Twelve patients diagnosed with PMT in our department and who underwent surgical treatment were included in this study. Preoperative demographic and clinical information were recorded. CT, MRI, and technetium-99m (Tc99m)-octreotide PET/CT imaging techniques were used to evaluate the general conditions and lesion boundaries of the tumors. Surgical treatment was performed using radical resection and microwave ablation-assisted extended curettage according to the lesion location and size. Patients were strictly followed up with and evaluated for oncological prognosis, radiological results, bone healing, serum ion levels, limb function, and pain level; the occurrence of complications was also recorded. Results: Three patients underwent radical resection, and nine underwent microwave ablation-assisted extended curettage. The average duration of symptoms in this group was 1.5 years (9–35 months) before diagnosis. Serum phosphate and AKP levels returned to normal one and two weeks postoperatively, respectively. There was no apparent specificity in the pathological findings; however, the immunohistochemistry of FGF-23 was positive, and the original fracture sites were effectively healed during the follow-up. The limb function and pain scores were significantly improved. The MSTS score increased from 15.3 to 29.0, and the VAS score decreased from 5.3 to 0.4. All patients recovered, and 90% resumed their original jobs. Conclusions: Accurate diagnosis and standardized surgical treatment are crucial to achieving a clinical cure for PMT. Combining clinical manifestations, biochemical examinations, imaging characteristics, and pathological findings is an effective way to diagnose PMT accurately. Radical resection and microwave ablation-assisted extended curettage are reliable surgical treatment methods for PMT.
Post-Cardiac Arrest: Mechanisms, Management, and Future Perspectives
Authors: Lazzarin Taline , Tonon Carolina Rodrigues , Martins Danilo , Fávero Edson Luiz , Baumgratz Thiago Dias , Pereira Filipe Welson Leal , Pinheiro Victor Rocha , Ballarin Raquel Simões , Queiroz Diego Aparecido Rios , Azevedo Paula Schmidt , Polegato Bertha Furlan , Okoshi Marina Politi , Zornoff Leonardo , Rupp de Paiva Sergio Alberto , Minicucci Marcos Ferreira
Published: December 2022

Cardiac arrest is an important public health issue, with a survival rate of approximately 15 to 22%. A great proportion of these deaths occur after resuscitation due to post-cardiac arrest syndrome, which is characterized by the ischemia-reperfusion injury that affects the role body. Understanding physiopathology is mandatory to discover new treatment strategies and obtain better results. Besides improvements in cardiopulmonary resuscitation maneuvers, the great increase in survival rates observed in recent decades is due to new approaches to post-cardiac arrest care. In this review, we will discuss physiopathology, etiologies, and post-resuscitation care, emphasizing targeted temperature management, early coronary angiography, and rehabilitation.
Albumin as a Prognostic Marker for Atrial Fibrillation Recurrence following Cryoballoon Ablation of Pulmonary Venous
Authors: Schamroth Pravda Nili , Golovchiner Gregory , Goldenberg Gustavo , Plakht Ygal , Wiessman Maya , Tal Shir , Barsheshet Alon , Kadmon Ehud , Erez Aharon , Skalsky Keren , Grinberg Tzlil , Nardi Agmon Inbar , Aviv Yaron , Kornowski Ran , Shiyovich Arthur , Hamdan Ashraf
Published: December 2022

Introduction: Atrial fibrillation (AF) recurrence following pulmonary vein isolation (PVI) ablation has clinical significance. Identifying risk factors for AF recurrence is important. We investigated serum albumin (SA) levels (g/dL) as a prognostic factor for the recurrence of AF following cryoballoon PVI ablation. Methods: We included patients who underwent cryoballoon PVI ablation at our institution between the years 2013 and 2018. The primary outcome was recurrence of AF during follow up. Results: Our cohort consisted of 126 patients (67% males, mean age 61.8 ± 10.0 years). The pattern of AF amongst the cohort was paroxysmal in 62.5%, persistent in 25.4%, and longstanding persistent in 6.3%. Those with lower SA levels had a mean AF duration significantly less than those with higher SA levels (2.81 years, 7.34 years, and 6.37 years for SA levels of <3.8, 3.8–4.1, and ≥4.1, respectively; p = 0.003). Patients with lower SA levels were significantly more likely to have had more previous cardioversions and a larger left atrial area and volume. The mean follow-up was 380 days, in which the AF recurrence rate was 20.6%. Patients with lower SA level had significantly more AF recurrences (47.4%, 16.7%, and 2.2% for SA levels of <3.8, 3.8–4.1, and ≥4.1, respectively; p < 0.001). Upon multivariate analysis, an SA level < 3.8 was associated with a higher risk of AF recurrence (OR = 5.422 95% CI 1.134; 25.910; p < 0.001). Conclusion: SA levels were found to be a strong independent marker for AF recurrence following PVI ablation.
Assessing the Mental Condition of Paramedics and Nurses during the SARS-CoV-2 Pandemic
Authors: Kilian Maksymilian , Gałecki Piotr , Orzechowska Agata
Published: December 2022

Background: The COVID-19 pandemic has significantly affected many aspects of life. The aim of this study was to assess the mental state of medical personnel most involved with patients affected by SARS-CoV-2. Methods: The survey research was conducted between October 2021 and March 2022. The study group included 46 paramedics, 48 nurses, and 6 individuals from both professions, ranging in age from 21 to 67 years. Data were collected using paper questionnaires that contained 44 questions. Results: During the pandemic, respondents maintained good contact with their colleagues and were competent enough to help their patients. The main factors that influenced their stress or dissatisfaction were the number of patients and the number of tasks. The nurses and paramedics most frequently reported symptoms related to tension, insomnia, and problems with intellectual performance. The pandemic situation led to the abandonment of hobbies and deterioration of relationships with friends and family. Conclusion: As a result of high stress levels, paramedics and nurses frequently suffered from tension and insomnia. The factors described were associated with poorer well-being of the subjects in various functional areas, even before the pandemic period. This was mainly related to the large number of patients and the tasks. However, this work, as well as studies by other authors, come to alarming conclusions that should draw attention to the mental state of medical staff, as this is the group that is highly responsible for the medical care of patients, especially in such a difficult time as a pandemic.
Multiparametric Magnetic Resonance Imaging Correlates of Isocitrate Dehydrogenase Mutation in WHO high-Grade Astrocytomas
Authors: Sahu Arpita , Patnam Nandakumar G. , Goda Jayant Sastri , Epari Sridhar , Sahay Ayushi , Mathew Ronny , Choudhari Amit Kumar , Desai Subhash M. , Dasgupta Archya , Chatterjee Abhishek , Pratishad Pallavi , Shetty Prakash , Moiyadi Ali Asgar , Gupta Tejpal
Published: December 2022

Purpose and background: Isocitrate dehydrogenase (IDH) mutation and O-6 methyl guanine methyl transferase (MGMT) methylation are surrogate biomarkers of improved survival in gliomas. This study aims at studying the ability of semantic magnetic resonance imaging (MRI) features to predict the IDH mutation status confirmed by the gold standard molecular tests. Methods: The MRI of 148 patients were reviewed for various imaging parameters based on the Visually AcceSAble Rembrandt Images (VASARI) study. Their IDH status was determined using immunohistochemistry (IHC). Fisher’s exact or chi-square tests for univariate and logistic regression for multivariate analysis were used. Results: Parameters such as mild and patchy enhancement, minimal edema, necrosis < 25%, presence of cysts, and less rCBV (relative cerebral blood volume) correlated with IDH mutation. The median age of IDH-mutant and IDH-wild patients were 34 years (IQR: 29–43) and 52 years (IQR: 45–59), respectively. Mild to moderate enhancement was observed in 15/19 IDH-mutant patients (79%), while 99/129 IDH-wildtype (77%) had severe enhancement (p-value <0.001). The volume of edema with respect to tumor volume distinguished IDH-mutants from wild phenotypes (peritumoral edema volume < tumor volume was associated with higher IDH-mutant phenotypes; p-value < 0.025). IDH-mutant patients had a median rCBV value of 1.8 (IQR: 1.4–2.0), while for IDH-wild phenotypes, it was 2.6 (IQR: 1.9–3.5) {p-value = 0.001}. On multivariate analysis, a cut-off of 25% necrosis was able to differentiate IDH-mutant from IDH-wildtype (p-value < 0.001), and a cut-off rCBV of 2.0 could differentiate IDH-mutant from IDH-wild phenotypes (p-value < 0.007). Conclusion: Semantic imaging features could reliably predict the IDH mutation status in high-grade gliomas. Presurgical prediction of IDH mutation status could help the treating oncologist to tailor the adjuvant therapy or use novel IDH inhibitors.
Does Farmland Tenancy Improve Household Asset Allocation? Evidence from Rural China
Authors: Xu Lijuan , Chandio Abbas Ali , Wang Jingyi , Jiang Yuansheng
Published: December 2022

In an agricultural society, the farmland is a major form of national wealth and an increase in farmland holding is a sign of wealth accumulation; whereas in an industrial society, the question of whether a rise in farmland holding also increases the wealth accumulation of farmers with the possible choice of being migrant workers is worth theoretical discussion and empirically testing. This article explores the issue of whether farmland tenancy affects household asset allocation in a rapid industrialization period. Using a sample of China’s rural households with land contract rights, we employed propensity score matching (PSM) with a difference-in-difference (DID) approach to explore and estimate the impact of farmland tenancy on tenant household asset allocation and test the mechanism of farmland tenancy affecting household asset allocation. Four conclusions are drawn from our study. (1) There is a ‘herd effect’ in the household decision-making in participation in the farmland rental market and the tenancy of farmland. (2) Household asset choice behavior is adjusted in response to the farmland tenancy for the tenants, increasing the investment in durable goods assets. (3) There is heterogeneity in the effects on asset allocation between tenant households with different intensities in farmland tenancy, presenting relatively more substantial impacts on the change of asset allocation for tenant households with high intensity in the farmland rental market. The tenancy of farmland with high intensity has not only boosted tenants’ wealth accumulation but has also increased the investment in agricultural assets and risky asset holdings (both incidence and the share of risky financial assets), while the tenancy of farmland with low intensity has not. Tenancy of farmland does not necessarily bring about an increase in the household’s agricultural machinery investment, and only when the area of farmland tenancy reaches a certain scale threshold will households increase their investment in agricultural machinery. (4) The tenancy of farmland has had impact on household asset allocation through a substitution effect instead of an income effect. In general, even though agriculture is not so profitable compared to industry in China, the wealth effect of farmland holding remains significant. This study contributes to the research on household asset allocation from the perspective of farm operation model transition caused by farmland market participation, which helps enhance income and accumulate wealth of rural households in China as well as other developing countries.
Influence of Long-Term Subcritical Annealing on the Unalloyed Steel Welded Joint Microstructure
Authors: Fajt Dominika , Maślak Mariusz , Stankiewicz Marek , Zajdel Paulina , Pańcikiewicz Krzysztof
Published: December 2022

The article presents changes in the microstructure of hot-rolled unalloyed structural steel after the arc welding process and in the state after long-term exposure to 600 °C during operation. These studies enable a clear assessment of the effects of long-term exposure to elevated temperature relative to the as-welded condition, which has not been reported. The microstructure examination was carried out on welded joints in eight different zones of the joint. Studies have shown that the welding thermal cycle causes significant changes in the microstructure in the area of the base material heated above the A1 temperature—the heat-affected zone (HAZ)—and in the weld area in the case of multi-pass welding. The long-term exposure of the subcritical temperature of 600 °C on the welded joint leads to the phenomenon of cementite spheroidization in the pearlite in all zones of the joint, while preserving the band structure of the steel after rolling and the structural structure. In the case of the weld, acicular and side-plate ferrite disappearance was observed.
Selected Determinants of Machines and Devices Standardization in Designing Automated Production Processes in Industry 4.0
Authors: Kuryło Piotr , Wysoczański Adam , Cyganiuk Joanna , Dzikuć Maria , Szufa Szymon , Bonarski Piotr , Burduk Anna , Frankovský Peter , Motyka Piotr , Medyński Daniel
Published: December 2022

The study presents a practical application of multi-criteria standardization of machines and devices in the design of the automated production processes in industry 4.0 and its direct impact on the economic aspects of an enterprise, along with a comparison of the state before and after the implementation of the proposed changes. The solutions recommended in the article also fit into the assumptions of low-carbon development by implementing solutions that reduce energy consumption. The research carried out and presented in the text confirmed the effectiveness of the described solution. The study also presents examples confirming the correctness of implementing standardization, synergy and coherence in the design of production processes. Additionally, a new advanced eLean application was presented to support production processes in the field of Lean Management. The Total Productive Maintenance (TPM) module currently implemented in the industry is concerned with ensuring the maximum efficiency of machines and devices.
Cement Bypass Dust as an Ecological Binder Substitute in Autoclaved Silica–Lime Products
Authors: Borek Katarzyna , Czapik Przemysław , Dachowski Ryszard
Published: December 2022

The cement industry is one of the most developed industries in the world. However, it consumes excessive amounts of natural resources and can negatively impact the environment through its by-products: carbon dioxide (CO2), cement clinker dust (CKD) and cement bypass dust (CBPD). The amount of dust generated in the cement clinker production process depends largely on the technology used. It typically ranges from 0 to 25% by weight of the clinker, and a single cement plant is capable of producing 1000 tons of CBPD per day. Despite practical applications in many areas, such as soil stabilisation, concrete mix production, chemical processing or ceramic and brick production, the dust is still stored in heaps. This poses an environmental challenge, so new ways of managing it are being sought. Due to the significant content of free lime (>30%) in CBPD, this paper uses cement bypass dust as a binder replacement in autoclaved silica–lime products. Indeed, the basic composition of silicate bricks includes 92% sand, 8% lime and water. The investigation shows that it is possible to completely replace the binder with CBPD dust in the autoclaved products. The obtained results showed that all properties of produced bricks were satisfactory. The study concluded that many benefits could be achieved by using cement bypass dust in the production of bricks, including economic bricks for building, reducing the dependency on natural resources, reducing pollution and reducing negative impacts on the environment.
Reliability and Validity of a Lithuanian Version of the Oral Health Impact Profile—A Study in Patients with Stage III–IV Periodontitis
Authors: Zasčiurinskienė Eglė , Šidlauskas Antanas , Kavaliauskienė Aistė , Vazgytė Jurgita , Matuzas Agnius , Zaborskis Apolinaras
Published: December 2022

Background and Objectives: The study aimed to translate the original English version of Oral Health Impact Profile (OHIP) into Lithuanian and to assess reliability and validity of the translated instrument (OHIP-Lt) in patients with advanced stages of periodontitis. Materials and Methods: Subjects (N = 67) with stage III–IV periodontitis aged 30–63 years were surveyed by questionnaire and examined clinically. Psychometric analysis included explanatory (EFA) and confirmatory (CFA) factor analyses and psychometric tests. Results: Cronbach’s alpha of the translated OHIP was 0.96. EFA revealed four dimensions which Cronbach’s alpha ranged from 0.75 to 0.96. Construct validity of the four-factor model derived from the OHIP-Lt was supported by findings of CFA (RMSEA = 0.077). The total OHIP-Lt and its subscale scores increased as the patients’ self-rated oral health status changed from healthy to unhealthy. Discriminative validity of the OHIP-Lt was confirmed by its higher scores among patients who had an increased spacing between the maxillary anterior teeth and increased clinical attachment level (CAL ≥ 5 mm) compared to those who did not. Conclusions: The translated Lithuanian version of OHIP-Lt was identified as four-dimension inventory. Good reliability and validity of the OHIP-Lt provide the evidence for its further use in study on advanced periodontal disease burden among Lithuanian patients.
Controlling Nutritional Status (CONUT) Score and Prognostic Nutritional Index (PNI) Are Good Candidates for Prognostic Markers for Acute Pancreatitis
Authors: Akkuzu Mustafa Zanyar , Altıntaş Engin , Yaraş Serkan , Sezgin Orhan , Ateş Fehmi , Üçbilek Enver , Özdoğan Osman
Published: December 2022

Background and Objectives: It has been demonstrated that parameters such as the Controlled Nutrition Status (CONUT) score and Prognostic Nutrition Index (PNI) are beneficial for the assessment of patients’ nutrition. In this study, our objective was to investigate the potential benefits of CONUT and, as a prognostic marker of acute pancreatitis, the PNI. Materials and Methods: The data of 361 patients were analysed retrospectively. The PNI and CONUT scores of these patients were retrospectively calculated. They were categorised as CONUT-high (≥3) and CONUT-low (≤2). A PNI ≥ 45 was considered high and a PNI < 45 low. The AP severity and organ failure due to disease were evaluated based on Atlanta 2012. Results: According to the CONUT score, it was found that 209 patients had normal to mild, whereas 152 patients had severe malnutrition. A total of 293 patients had mild AP and 68 thereof had severe AP. The patients with a high CONUT score used more antibiotics, were hospitalised more in intensive care units and experienced organ failure more frequently. There were no intensive care hospitalisations, mortalities, surgical needs and local complications among the patients with a higher PNI score. Conclusions: CONUT and the PNI have proven to be useful prognostic markers not only for predicting nutritional status but also for estimating the severity and results of AP.