bluuebunny/crossref_metadata_2025 huggingface dataset:
STRATEGY OF DEVELOPING TOURISM BEACH (CASE STUDY: LAGUNA BEACH, KAUR REGENCY)
Authors: Nengsih Mimi Kurnia , Er Meytha Gayatri Ida Ayu Made
Published: March 2022

Kaur District has desperately attractive potential beach tourism and also has the opportunity to bring local and foreign tourists and increase the locally-generated revenue. This potential tourism is the Laguna Beach. The objective of study was to determine the SWOT analysis that is implemented to develop strategy of Laguna Beach tourism in Kaur District. The data were collected by distributing questionnaires to respondents. The analytical method used is SWOT analysis to determine the internal factors (strength and weakness) and external factors (opportunity and threat), which will be incorporated into the SWOT matrix. The results of study showed the analysis of internal and external factors. The internal factor was strength with a total score of 22.2 and weakness with a total score of 3.7 so the internal factors achieved the value of 18.5. For the external factors, the opportunity had a total score of 16.96 while the threat had a total score of 8.41 so the external factors achieved the value of 8.55. According to SWOT diagram analysis, the marketing strategy of Laguna Beach in Kaur District can be placed in Quadrant I. This is shown by SO strategy in SWOT matrix that is Aggressive Strategy. This strategy can develop Laguna Beach because it has opportunity and strength in the future, thus it can increase the number of visitors/tourists at Laguna Beach.
FACTORS INFLUENCING INTENTION TO VISIT SOUTH KOREA DURING THE PANDEMIC
Authors: Noviyati Nabila Andena , Asih Ratna Asih , Putri Ramadhanti Salsabila , Fakhrunnisa Fakhrunnisa , Suhud Usep
Published: March 2022

This study examines the factors that influence the intention to visit South Korea during the pandemic. This study uses six measured variables: perceived benefits, destination image, e-word of mouth, destination service quality, culture, and visit intention. This study used a quantitative model by collecting data using an online questionnaire and with the criteria for female or male respondents aged 25 years and over and visiting South Korea during the pandemic. The data collection was carried out in January 2021. The total respondents in this study were 212 people consisting of 45 men and 167 women. The results obtained from this study, namely destination service quality has a positive effect on e-word of mouth, e-word of mouth has a positive impact on destination image.
IMPLEMENTATION AND TOURIST PERCEPTION OF SAPTA PESONA AT TAMAN KUPU-KUPU, SUKAJADI TOURISM VILLAGE, BOGOR
Authors: Ishak Riani P.
Published: March 2022

This study aims to determine, describe, and analyze (1) the implementation of Sapta Pesona at Taman Kupu-Kupu in the Sukajadi Tourism Village and (2) the tourists' perception of Sapta Pesona actualization at Taman Kupu-Kupu in the Sukajadi Tourism Village. The research method used in this research is descriptive research with a mixed approach. Data collection techniques used are observation, interviews, questionnaires, and documentation and literacy. The analysis method used is (1) Miles and Huberman qualitative data analysis in the form of data reduction, data presentation, and drawing conclusions. Then the second is quantitative data analysis with descriptive statistical analysis using SPSS. The sample in this study were tourists who visited the Taman Kupu-Kupu in Sukajadi Tourism Village in May 2021, which is limited due to pandemic Covid-19. The results of this study indicate that (1) the application of Sapta Pesona Taman Kupu-Kupu Sukajadi Tourism Village on the seven elements has generally been carried out well, however, each indicator still can be improved to the most optimal level, in particular for elements safe, clean, cool, and beautiful. Furthermore, a survey on tourist perceptions in regard to the implementation of Sapta Pesona at Taman Kupu-Kupu Sukajadi Tourism Village is rated excellent on these elements: orderliness,  friendliness, and memorable.  Various efforts can still be worked on to reach maximum value for Sapta Pesona implementation. By improving its organizational strategy, providing skills training for its employee, preparing written and displayed Standard Operating Procedure in the park, building cooperation with related parties and improving marketing and promotion strategy are some recommendations can be conducted by the management of Taman Kupu-Kupu Sukajadi Tourism. Based on the results of this study. Moreover, it is strongly recommended that the Bogor Regency Tourism and Culture Office should pay more attention to conducting regular supervision of developing tourism villages in Bogor so that the implementation of Sapta Pesona can be optimized.

 Keywords: Sapta Pesona, Tourist Perception, Tourism Village , Sukajadi Village
ANALYSIS OF MARKETING ASPECT IN FEASIBILITY STUDY OF ASIAN CULTURE GALLERY IN WEST JAKARTA
Authors: Sitorus Nova , Juliana Juliana , Kho Chaterine
Published: March 2022

Based on the data and explanation above, the writer decided to make a business feasibility study from the marketing aspect of an art gallery. The art gallery that will be discussed in this business feasibility study is entitled Asian Culture Gallery. The Asian Culture Gallery, located in West Jakarta, is an art gallery that displays various paintings and explanations of traditional culture, such as traditional dances, special foods, official languages ​​and also traditional clothing from various countries in Asia. These countries include South Korea, Japan, Indonesia, India and the Middle East. In order to produce supporting data, reliable data sets and sources are needed. Therefore, the data obtained must be valid and reliable data. The business feasibility study of the Asian Culture Gallery located in West Jakarta collects data using scientific methods, namely primary data and secondary data. This art gallery will also display some examples of paintings, information and products from the country concerned. The Asian Culture Gallery questionnaire has been distributed online to 153 respondents to find out market interest and responses to questions or statements given. From a market perspective, this gallery is worth running.

Keyword: Market, Feasibikity, Gallery, Business
Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs
Authors: Liao Yunyang , Fang Youran , Zhu Hanghang , Huang Yue , Zou Gengsen , Dai Bowen , Rausch Macro Aoqi , Shi Bin
Published: March 2022

Osteogenesis is a complex physiologic process that occurs during bone regeneration. This process requires several growth factors that act on bone marrow-derived mesenchymal stem cells (BMSCs). Concentrated growth factor (CGF) is a new-generation platelet-rich derivative that is an appealing autologous material for application in tissue repair and bone regenerative medicine because it contains a variety of fibrin and growth factors. In this study, the effects of CGF on the proliferation and osteogenic differentiation of hBMSCs and human umbilical vein endothelial cells (HUVECs) were explored with in vitro cell co-culture experiments. HBMSCs and HUVECs were directly co-cultured at the ratio of 1:2 under different concentrations (0, 2, 5, 10, 20%) of CGF for 7 days. Alkaline phosphatase (ALP) staining and quantitative reverse transcription polymerase chain reaction were used to detect the effects of CGF on the expression of osteogenic genes (ALP, osteocalcin [OCN], type I collagen [COL-1], Runt-related transcription factor 2 [RUNX2]) and connexin 43 (CX43). RNA sequencing was used to explore potential regulated genes and signaling pathways that affect the osteogenesis of co-cultured hBMSCs exposed to CGF. The results showed higher expressions of ALP, COL-1, RUNX2, OCN, and CX43 in the direct co-culture group containing 10% CGF compared to the direct co-culture group without CGF and the indirect co-culture group. In summary, 10% CGF can significantly promote osteogenesis in hBMSCs directly co-cultured with HUVECs. Intercellular communication between the direct co-culture of hBMSCs and HUVECs through CX43 may be one of the main regulatory mechanisms.
Geochemical and Isotopic Characteristics of Two Geothermal Systems at the Nanpu Sag, Northern Bohai Bay Basin
Authors: Wang Ke , Hua Cong , Ren Lu , Kong Yanlong , Sun Wenjie , Pan Sheng , Cheng Yuanzhi , Huang Yonghui , Tian Fei , Zhang Weizun , Qin Dajun , Ma Feng , Wang Jianwei , Dong Yuexia
Published: March 2022

The utilization of geothermal energy has gradually increased in northern China because of its unique advantages as a heating supply. However, the sustainable exploitation of geothermal energy usually requires a comprehensive investigation of the geothermal water circulation pattern prevailing at a proposed site. During the exploitation of geothermal energy resources at Nanpu Sag in northern China, thermal anomalies were found to exist in two adjacent regions: the Caofeidian and the Matouying. To reconcile the anomalies and to examine both the source of recharge water and the geothermal systems’ circulation dynamics, a comprehensive investigation was performed using multiple chemical and isotopic tracers (δ2H, δ18O, 87Sr/86Sr, δ13C, and 14C). The total dissolved solids (TDS) of the geothermal water are approximately 750 mg/L and 1,250 mg/L, respectively. The geothermal water isotopes at the two sites are also different, with average values of -9.3‰ and -8.2‰ for δ18O and -73.4‰ and -71‰ for δ2H, respectively. Moreover, the 87Sr/86Sr ratio of geothermal water at Matouying is 0.7185, which is much greater than that of Caofeidian, with an average value of 0.7088. All the results confirm the difference between the two geothermal systems and may explain the two circulation patterns of deep groundwater at Caofeidian and Matouying. The reservoir temperature obtained from theoretical chemical geothermometers is estimated to be 83–92°C at the Caofeidian and 107–137°C at the Matouying, respectively. The corrected 14C age implies a low circulation rate that would allow sufficient time to heat the water at Caofeidian. In addition, we propose a geothermal conceptual model in our study area. This model could provide key information regarding the geothermal sustainable exploitation and the effective management of geothermal resources.
Earthquake Occurrences of the Major Tectonic Terranes for the Arabian Shield and Their Seismic Hazard Implications
Authors: Ali Sherif M. , Abdelrahman Kamal
Published: March 2022

The Arabian Shield, which contains a group of diverse terranes accreted during the Late Proterozoic, has experienced considerable historical and recent earthquake activities. From north to south, the Midyan terrane, Tabuk-Neom area, Hijaz terrane, Jeddah terrane, and Asir terrane make up the western section of the Arabian shield. In order to determine the earthquake occurrences and earthquake recurrence characteristics in the study area, an earthquake dataset containing 2,991 seismic events recorded between 1941 and 2019 with magnitudes of 1.0 and 6.2 and depths between 0 and 50 km was examined. The data were compiled by combining phase readings and information, such as origin times, hypocenter parameters, and magnitudes reported by the International Seismological Centre. The maximum likelihood method has been applied to calculate the Gutenberg–Richter recurrence parameters (a- and b-values) and magnitudes of completeness (Mc). The range of b-values is 0.53–1.04, which indicates that the study region experienced different stress level accumulations that cause earthquakes with different magnitudes. The Hijaz terrane is characterized by a high b-value (1.04 ± 0.34), which indicates a relatively low stress regime that resulted from the earthquakes stress release. The Midyan terrane is characterized by a low b-value (0.53 ± 0.10), which could be indicative of a relatively higher stress regime associated with a dominantly extensional stress. Mc values were found to be 1.4 in Midyan and Jeddah terranes. The lower value of Mc at Midyan terrane demonstrates appropriate station distribution and high earthquake rates. However, at Jeddah terrane, the seismic activities are poorly detected that probably lead to the small value of Mc. Higher Mc are evident in Hijaz terrane (Mc = 2.3) and Tabuk-Neom (Mc = 2.4), where the station distribution is very poor. The maximum expected magnitudes (Mmax) are found to be 6.0 for Midyan terrane, 5.4 for Tabuk-Neom, 4.7 for Hijaz terrane, 4.8 for Jeddah terrane, and 7.7 for Asir terrane. The average recurrence intervals of earthquakes with the Mmax are ∼7, ∼20, ∼6, ∼120, and ∼200 years for each seismic terrain, respectively. The probability of occurrence and returned periods of different magnitudes in each region indicate that regions related to the Najd strike-slip fault system are the regions for large probable earthquake occurrences.
Production Performance Analysis for Deviated Wells in Carbonate Gas Reservoirs With Multiple Heterogeneous layers
Authors: Guo Jianlin , Ning Bo , Yan Haijun , Jia Chengye , Li Yilong
Published: March 2022

Deviated wells are used to improve the performance of carbonate reservoirs with multiple heterogeneous layers and penetrate the “sweet spot” of each layer, which is full of fractures and vugs. It is difficult to consider in-layer and inter-layer heterogeneities simultaneously, and predict the production performance for these wells accurately. Therefore, a semi-analytical model to analyze the production performance of deviated wells in a multilayer heterogeneous stress-sensitive carbonate gas reservoir is proposed. For each layer, the inner region is a fractured-vuggy porous medium, while the outer region is merely a tight formation with matrix and formation properties, and penetrated inclination angles may be distinct. Pseudo-time/pressure factors are introduced to consider fracture stress sensitivity. Through the application of Laplace transformation, Fourier transform and inverse, Duhamel convolution, and Stehfest numerical inversion, the presented model is solved. The validity of this model is verified through comparison with single-layer composite formation with different porous mediums and vertical well in a multilayer carbonate gas reservoir. Moreover, by matching bottom-hole pressure data collected from a slanted well in the Anyue gas field, the applicability of this model is validated. A synthetic case, which has two composite formations, the first (upper) layer is more permeable than the second (lower) layer, is used to study the variations of inner region radius, fracture/matrix permeability, and inclination angles on production behaviors. The results show the properties of the first layer determine well bottom-hole pressure, whereas the rise of permeability, inner region radius and penetrated angle for the second layer can improve the gas recovery of this layer. In practice, to maintain well bottom-hole pressure with a relatively high level and enhance gas recovery of the tight layer, the inclination angle should be larger than 60° for each layer, and be increased to as large as possible. The findings of this study can help for a better understanding of the production behaviors of deviated wells in multilayer heterogenous reservoirs and could provide some guidance for the design of well trajectory.
Role of DTL in Hepatocellular Carcinoma and Its Impact on the Tumor Microenvironment
Authors: Li Zuyin , Wang Rangrang , Qiu Chen , Cao Can , Zhang Jianming , Ge Jun , Shi Yuanping
Published: March 2022

Background

The crucial role of DTL has been previously implicated in genomic stability; however, its prognostic value and its relation with tumor immunity in hepatocellular carcinoma (HCC) remain to be further explored.

Methods

Transcriptional and mutational datasets as well as clinical information were retrieved from the GEO, ICGC, and TCGA databases. Differentially expressed genes (DEGs) were obtained from the comparison of DTLhigh and DTLlow expression groups of the TCGA-HCC cohort. Those genes were under KEGG and gene ontology (GO) analyses to decipher the influence of the DTL gene on the biological behavior of HCC tumor cells. The survival status and mutational characteristics of patients according to DTL levels were depicted and analyzed. The DTL overexpression in HCC and its impact on prognosis were further confirmed by a cohort of 114 HCC patients (validation cohort). The TIMER, GEPIA, and TISIDB databases were adopted to investigate the potential relations between DTL levels and the status of immune cells, as well as immune cell infiltrations.

Results

The DTL gene is overexpressed in tumor tissues compared with distant non-malignant liver tissues, and DTL overexpression in HCC would enhance the HCC cells in the activities of cell cycle and division. HCC patients with high DTL expression have unfavorable clinical outcomes and harbor more somatic mutations than those with low DTL expression, and multivariate analysis also revealed that DTL overexpression could act as an independent biomarker for prognosis. Moreover, the DTL gene was positively linked to marker sets of infiltrating activated CD8+ and CD4+ T cells; however, these cells demonstrated to be functionally exhausted.

Conclusions

Patients with a DTL overexpression phenotype in HCC have poorer prognosis than those in the DTLlow group due to the role of the DTL gene in the process of pro-cell proliferation, accompanied by the immunosuppressive microenvironment and T cell exhaustion.
Effect of Slag Gypsum Binder as a Substitute to Cement on the Stability of Backfill Mining
Authors: Xiaobing Yang , Zepeng Yan , Weiguang Li , Leiming Wang , Shenghua Yin
Published: March 2022

To effectively apply slag-gypsum binder (SGB) to most modern mines. An experimental study is conducted to investigate the feasibility and reliability of slag-gypsum binder (SGB) to replace cement in the mine backfill to effectively apply SGB in modern mines. The optimum parameters of aggregate and SGB fineness were investigated on a laboratory basis and the effect of concentration on the workability was analyzed. Also, industrial tests were carried out to study the cementitious properties of SGB and compared to cement. Finally, the hydration properties of SGB were analyzed using XRD and SEM. The results indicated that the main hydration products of SGB are C-S-H gel and ettringite, while Ca(OH)2 is consumed during the hydration process. In addition, the structure formed in the later stage of SGB is more compact. SGB is beneficial to the strength of the waste rock mixture. The sample prepared with the waste rock-rod milling sand-river sand mixture has a maximum strength of 7.54 MPa at 28 d. Reducing fineness is beneficial to form early strength. However, a specific surface area exceeding 510 m2/kg will harm the later strength. Surface area is less (400 m2/kg), the sample strength decreases when the dosage increases. Comparing the workability of SGB slurry and cement slurry, it is found that there is no essential difference between them under different mass concentration conditions. SGB slurry shows better fluidity and stability at a mass concentration of 80–82%. Industrial backfill experiments show the 3-, 7-, and 28-d strengths of SGB samples under 80% mass concentration conditions are 0.91 times, 1.43 times, and 1.2 times the cement samples. Therefore, using SGB instead of cement to backfill is a good application prospect.
Levels of Soluble NKG2D Ligands and Cancer History in Patients Starting Hemodialysis
Authors: Nagai Kei , Tawara Takashi , Usui Joichi , Ebihara Itaru , Ishizu Takashi , Kobayashi Masaki , Maeda Yoshitaka , Kobayashi Hiroaki , Yamagata Kunihiro
Published: March 2022

Background

Immune dysfunction in hemodialysis patients is partially due to NK cell impairment. Ligands for NK activating receptors such as NKG2D expressed on cancer cells are involved in NK cell dysfunction and can lead to cancer development.

Methods

A cohort with 370 patients who started hemodialysis (HD) was investigated. Serum levels of soluble NKG2D ligands were measured. Cancer history was defined as any cancer diagnosis at induction and hospitalization and death due to cancer during 2-year follow-up.

Results

Sixty-two patients with and 308 patients without a cancer history showed mostly comparable biochemical parameters and uremic status at HD induction. Soluble MICB, ULBP-1, and ULBP-2 were detected in sera from most patients starting HD rather than MICA, the most representative NKG2D ligand. Measured NKG2D ligands, except for ULBP-1, were strongly correlated with each other. Correlations between NKG2D ligands and renal function were significant but modest in patients starting HD. Cancer history did not have any impact on levels of soluble NKG2D ligands.

Discussion

Even though this investigation lacked a control cohort and serial measurement of parameters, expression patterns of NKG2D ligands were comprehensively described, and the significance of cancer in patients starting HD was elucidated for the first time. Elevated levels of soluble NKG2D ligands occurred potentially due to complex mechanisms of oxidative stress, with insufficient metabolism and excretion in a uremic milieu, but they might mask the significance of elevations in serum levels of soluble NKG2DLs in patients with a cancer history.
Early Cervical Lesions Affecting Ovarian Reserve and Reproductive Outcomes of Females in Assisted Reproductive Cycles
Authors: Yang Qiyu , Hu Juan , Wang Meng , Li Zhou , Huang Bo , Zhu Lixia , Xi Qingsong , Jin Lei
Published: March 2022

To estimate the effects of early cervical lesions (ECL) on female reproductive function and IVF/ICSI cycle outcomes, a retrospective cohort study involving 111 infertile women from 2014 to 2019 was performed. Thirty-seven women with a history of ECL and seventy-four controls, undergoing IVF/ICSI cycles, were included in the ECL group and comparison group respectively. Demographic characteristics, ovarian reserve, and IVF/ICSI cycle outcomes of both groups were collected. Basal serum FSH level, AMH level, AFC, number of oocytes retrieved and matured, normal fertilization rate, embryo available rate, blastocyst formation rate, implantation rate, pregnancy rate, and cumulative live birth rate (CLBR) were assessed and compared. We observed that while both groups were similar concerning baseline features, significantly more women in the ECL group were diagnosed as poor ovarian response (POR), compared with those in the comparison group (27.0% vs. 10.8%, P=0.003). The pregnancy rate and LBR for a complete cycle were both significantly lower in the ECL group (38.5% vs. 58.8%, P=0.021; 28.9% vs. 48.2%, P=0.025, respectively). The conservative and optimal CLBRs for up to four complete cycles in the ECL group were also lower than those in the comparison group (40.5% vs. 55.4%, P=0.140; 45.9% vs. 67.6%, P=0.028). Longer time intervals (over one year) between ECL diagnosis/treatment and assisted reproductive technology (ART) cycle start negatively affected the pregnancy rate and LBR. In conclusion, female patients with ECL history seemingly have a lower ovarian reserve, reduced pregnancy rate, and decreased live birth rate (LBR), compared with age-matched women undergoing IVF/ICSI.
Development and Evaluation of the Quality of Life Scale for Children With Visual Impairments in China
Authors: Liu Jie , Evans Richard , Wang Yanjun , Hu Beibei , Tong Yan , Li Shaoqiong , Tian Zhiqiang , Li Jing , Zhang Cuihua , He Lu , Zheng Jianzhong
Published: March 2022

Background

Visual impairments related to non-correctable vision loss, including blindness and low vision, have been consistently shown to lower a person's health-related quality of life. This study assessed the reliability, validity, and discrimination of the Quality of Life Scale for Children with Visual Impairments (QOLS-CVI) in China.

Methods

The Pediatric Quality of Life Inventory™ 4.0 and World Health Organization Quality of Life-Disability Scale for physical disability were selected to define conceptual frameworks and item libraries based on relevant existing studies. According to two rounds of expert consultations and group discussions, some items were modified, and the draft scale was developed. Two item selection processes based on classical test theory and item response theory were used to conduct a preliminary survey and a formal survey in special schools in Shanxi and Hebei Provinces. Finally, the reliability and validity of the quality of life scale for visually impaired children in China were verified.

Results

The final QOLS-CVI consisted of 38 items, 10 subdomains, and 6 domains. Reliability was verified by Cronbach's alpha coefficient, split-half reliability, and test-retest reliability (Cronbach's alpha for the full scale, 0.841; split-half reliability, 0.629; and test–retest reliability, 0.888). The validity results showed that the multidimensional scale met expectations: exploratory factor analysis and confirmatory factor analysis indicated good fitting models for children with visual impairments.

Conclusions

The QOLS-CVI was determined to be reliable and valid and to have strong feasibility and effectiveness. This scale can be used as an evaluation tool to study the QOL and social-participation ability of children with visual impairments.
Effects of High-Pressure Processing, UV-C Irradiation and Thermoultrasonication on Donor Human Milk Safety and Quality
Authors: Kontopodi Eva , Stahl Bernd , van Goudoever Johannes B. , Boeren Sjef , Timmermans Rian A. H. , den Besten Heidy M. W. , Van Elburg Ruurd M. , Hettinga Kasper
Published: March 2022

Holder pasteurization (HoP) is the current recommended treatment for donor human milk. Although this method inactivates microbial contaminants, it also negatively affects various milk components. High-pressure processing (HPP, 400, 500, and 600 MPa), ultraviolet-C irradiation (UV-C, 2,430, 3,645, and 4,863 J/L) and thermoultrasonication (TUS, 1,080 and 1,620 kJ/L) were investigated as alternatives to thermal pasteurization (HoP). We assessed the effects of these methods on microbiological safety, and on concentration and functionality of immunoglobulin A, lactoferrin, lysozyme and bile salt-stimulated lipase, with LC-MS/MS-based proteomics and activity assays. HoP, HPP, TUS, and UV-C at 4863 J/L, achieved >5-log10 microbial reduction. Native protein levels and functionality showed the highest reduction following HoP, while no significant reduction was found after less intense HPP and all UV-C treatments. Immunoglobulin A, lactoferrin, and lysozyme contents were also preserved after low intensity TUS, but bile salt-stimulated lipase activity was significantly reduced. This study demonstrated that HPP and UV-C may be considered as suitable alternatives to HoP, since they were able to ensure sufficient microbial inactivation while at the same time better preserving the bioactive components of donor human milk. In summary, our results provide valuable insights regarding the evaluation and selection of suitable processing methods for donor human milk treatment, which may replace HoP in the future.
The IRE1α Inhibitor KIRA6 Blocks Leukotriene Biosynthesis in Human Phagocytes
Authors: Tang Xiao , Teder Tarvi , Samuelsson Bengt , Haeggström Jesper Z.
Published: March 2022

The ER stress and Unfolded Protein Response (UPR) component inositol-requiring enzyme 1α (IRE1α) has been linked to inflammation and lipid mediator production. Here we report that the potent IRE1α inhibitor, KIRA6, blocks leukotriene biosynthesis in human phagocytes activated with lipopolysaccharide (LPS) plus N-formyl-methionyl-leucyl-phenylalanine (fMLP) or thapsigargin (Tg). The inhibition affects both leukotriene B4 (LTB4) and cysteinyl leukotriene (cys-LTs) production at submicromolar concentration. Macrophages made deficient of IRE1α were still sensitive to KIRA6 thus demonstrating that the compound’s effect on leukotriene production is IRE1α-independent. KIRA6 did not exhibit any direct inhibitory effect on key enzymes in the leukotriene pathway, as assessed by phospholipase A2 (PLA2), 5-lipoxygenase (5-LOX), LTA4 hydrolase (LTA4H), and LTC4 synthase (LTC4S) enzyme activity measurements in cell lysates. However, we find that KIRA6 dose-dependently blocks phosphorylation of p38 and ERK, mitogen-activated protein kinases (MAPKs) that have established roles in activating cytosolic PLA2α (cPLA2α) and 5-LOX. The reduction of p38 and ERK phosphorylation is associated with a decrease in cPLA2α phosphorylation and attenuated leukotriene production. Furthermore, KIRA6 inhibits p38 activity, and molecular modelling indicates that it can directly interact with the ATP-binding pocket of p38. This potent and unexpected, non-canonical effect of KIRA6 on p38 and ERK MAPKs and leukotriene biosynthesis may account for some of the immune-modulating properties of this widely used IRE1α inhibitor.
Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats
Authors: Alaei Faradonbeh Fatemeh , Lastuvkova Hana , Cermanova Jolana , Hroch Milos , Nova Zuzana , Uher Martin , Hirsova Petra , Pavek Petr , Micuda Stanislav
Published: March 2022

Multidrug resistance-associated protein 2 (Mrp2) mediates biliary secretion of anionic endobiotics and xenobiotics. Genetic alteration of Mrp2 leads to conjugated hyperbilirubinemia and predisposes to the development of intrahepatic cholestasis of pregnancy (ICP), characterized by increased plasma bile acids (BAs) due to mechanisms that are incompletely understood. Therefore, this study aimed to characterize BA metabolomics during experimental Mrp2 deficiency and ICP. ICP was modeled by ethinylestradiol (EE) administration to Mrp2-deficient (TR) rats and their wild-type (WT) controls. Spectra of BAs were analyzed in plasma, bile, and stool using an advanced liquid chromatography–mass spectrometry (LC–MS) method. Changes in BA-related genes and proteins were analyzed in the liver and intestine. Vehicle-administered TR rats demonstrated higher plasma BA concentrations consistent with reduced BA biliary secretion and increased BA efflux from hepatocytes to blood via upregulated multidrug resistance-associated protein 3 (Mrp3) and multidrug resistance-associated protein 4 (Mrp4) transporters. TR rats also showed a decrease in intestinal BA reabsorption due to reduced ileal sodium/bile acid cotransporter (Asbt) expression. Analysis of regulatory mechanisms indicated that activation of the hepatic constitutive androstane receptor (CAR)-Nuclear factor erythroid 2-related factor 2 (Nrf2) pathway by accumulating bilirubin may be responsible for changes in BA metabolomics in TR rats. Ethinylestradiol administration to TR rats further increased plasma BA concentrations as a result of reduced BA uptake and increased efflux via reduced Slco1a1 and upregulated Mrp4 transporters. These results demonstrate that Mrp2-deficient organism is more sensitive to estrogen-induced cholestasis. Inherited deficiency in Mrp2 is associated with activation of Mrp3 and Mrp4 proteins, which is further accentuated by increased estrogen. Bile acid monitoring is therefore highly desirable in pregnant women with conjugated hyperbilirubinemia for early detection of intrahepatic cholestasis.
The Effect of Anti-browning Agent Activated Carbon and Polyvinyl Pyrrolidone on the Rooting of Embryo Seedlings of “FengDan” and Its Transcriptome Analysis
Authors: Chen Xia , Yu Chao , Nie Jingtao , Yang Hongmin , Ji Wen , Xu Gangwei , Zhu Huijun , Jin Songheng , Zhu Xiangtao
Published: March 2022

Peony is an excellent ornamental, medicinal, and oily plant. Its traditional seed propagation methods have the disadvantages of low propagation coefficient, long seedling cycle, and low seedling emergence rate, which severely restrict the supply of seedlings for the peony industry. Efficient tissue culture technology is an important basis for accelerating its breeding and reproduction, and in vitro seed embryo culturing into seedlings can also effectively avoid the above problems. However, the browning phenomenon caused by man-made damage in the process of seed embryo stripping leads to problems such as low induction rate and difficulty in rooting, and the relationship between anti-browning agents and seed embryo root formation is still unclear. This study intends to improve the induction rate of peony seedlings by using different anti-browning agents and different combinations and to clarify the relationship between anti-browning agents and seedling rooting using transcriptome sequencing methods. The results show that both anti-browning agents, activated carbon (AC) and polyvinyl pyrrolidone (PVP), can increase the germination rate of seed embryos. Testing with 0.9 g/L of AC showed excellent performance of peony rooting rate and seedling growth, but only AC and the combination of AC and PVP can further promote rooting development. Through transcriptome analysis, we found that the AC vs. control check (CK), AC vs. PVP, and PVP vs. AC and PVP groups have significantly more differentially expressed genes than the AC vs. AC and PVP groups. Pathway enrichment analysis shows that “phenylpropanoid biosynthesis”/“cutin, suberin, and wax biosynthesis” is significantly enriched in these groups, while the AC vs. AC and PVP groups are mainly enriched in “cytochrome P450,” indicating that AC may promote the further development of roots into seedlings by stimulating “phenylpropanoid biosynthesis” and biosynthesis of stratum cutin and suberin. This study can lay the foundation for understanding the potential molecular mechanism of the anti-browning agent promoting the rooting of seed embryo seedlings and also provide a theoretical basis for perfecting the construction of the peony tissue culture and rapid propagation system.
Shared Control Schemes for Middle Ear Surgery
Authors: So Jae-Hun , Sobucki Stéphane , Szewczyk Jérôme , Marturi Naresh , Tamadazte Brahim
Published: March 2022

This paper deals with the control of a redundant cobot arm to accomplish peg-in-hole insertion tasks in the context of middle ear surgery. It mainly focuses on the development of two shared control laws that combine local measurements provided by position or force sensors with the globally observed visual information. We first investigate the two classical and well-established control modes, i.e., a position-based end-frame tele-operation controller and a comanipulation controller. Based on these two control architectures, we then propose a combination of visual feedback and position/force-based inputs in the same control scheme. In contrast to the conventional control designs where all degrees of freedom (DoF) are equally controlled, the proposed shared controllers allow teleoperation of linear/translational DoFs while the rotational ones are simultaneously handled by a vision-based controller. Such controllers reduce the task complexity, e.g., a complex peg-in-hole task is simplified for the operator to basic translations in the space where tool orientations are automatically controlled. Various experiments are conducted, using a 7-DoF robot arm equipped with a force/torque sensor and a camera, validating the proposed controllers in the context of simulating a minimally invasive surgical procedure. The obtained results in terms of accuracy, ergonomics and rapidity are discussed in this paper.
Trainings in financial culture : A comparative analysis (2016–2020)
Authors: Németh Erzsébet
Published: March 2022

The aim of the research is to assess whether any progress has been made in developing financial awareness in Hungary since 2016. The study presents the changes in the state’s role in the development of financial culture, in the framework of a questionnaire survey, it examines what kind of organisations provide trainings in the form of non-public education, to whom such programmes are provided and what syllabus they are based on, as well as it evaluates the quality assurance of the programmes. Between 2016 and 2020, there was an increasing focus on developing financial culture, while most courses are still aimed at the most accessible group of school-aged children. The National Core Curriculum identified economic and financial education as a goal for schools. However, apart from secondary vocational schools, other schools do not teach such subjects as compulsory subjects. In 2017, the Government adopted a strategy to improve the financial awareness of the population, and the first accredited financial literacy textbooks were published. The results of the non-public educational organisations show that the number of training programmes and participants has tripled. The average duration of trainings has become longer. In particular, multi-day courses provided mainly to adults have appeared. The focus of knowledge transfer continues to be on individual savings and financial awareness, financial self-knowledge, attitude and behaviour. Four-fifths of the courses have a curriculum and a syllabus, but most of them are not accredited and are not publicly available. About 40% of the courses measure effectiveness.
Ideas on teaching finance and accounting in tertiary education : What should future bankers and accountants learn?
Authors: Lantos Csaba , Lukács János , Száz János
Published: March 2022

Designing financial education at the post-secondary level is a key component of financial culture. We are investigating in this paper what future bankers, accountants, or auditors should learn to boost the prestige of their professions, to have great value for money with their degrees, and maintain their favourable positions on the job market. All institutions must prepare for fierce competition in this country as demographic indicators are worsening and competition from abroad is intensifying. The global picture is true for our field too; institutions of tertiary financial education must find their places and select their strategy. Recalling historical examples, we present views and perspectives we believe should be considered when making decisions affecting the future.
Search for preventive strategies in the fight against smoking based on the analysis of smoking rates among medical students
Authors:   FSAEI HE Peoples' Friendship University of Russia, Zastrozhina Anastasiya Konstantinovna , Drozhzhina Nataliya Agafonovna ,   FSAEI HE Peoples' Friendship University of Russia, Zaitseva Svetlana Vladimirovna ,   FSBEI HE A. I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia, Galkina Mariya Ilyinichna ,   FSAEI HE Peoples' Friendship University of Russia, Chuksina Polina Sergeevna ,   FSAEI HE Peoples' Friendship University of Russia, Kulmuradov Azat Babamuradovich ,   Medical Institute, FSAEI HE Peoples' Friendship University of Russia, Shirkovets Arina Alekseevna ,   Medical Institute, FSAEI HE Peoples' Friendship University of Russia
Published: March 2022

In recent years, the problem of smoking is still relevant. The advent of electronic smoking devices on the market has led to a significant increase in their use, especially among young people. The undeniable social and economic hazards, morbidity and mortality associated with smoking require an analysis of the causes and factors affecting the increase in the prevalence of various types of smoking, which will help to set the focus in the development of preventive measures. In this study, based on an interview of medical students, an analysis of the prevalence of smoking among young people was carried out, the frequency of occurrence and reasons for choosing different types of devices for smoking were evaluated, the age of initiation of smoking, as well as the reasons for not being able to give up smoking were studied. On the basis of the data obtained, directions to focus attention in the development of preventive measures against smoking have been proposed.
Calculation of mercury intake from the consumption of wild fish by the population of the Vologda region
Authors:   Cherepovets State University, Ivanova Elena Sergeevna , Eltsova Lyubov Sergeevna ,   Cherepovets State University,, Shuvalova Olesya Petrovna ,   Cherepovets State University, Komov Viktor Trofimovich ,   I.D. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences (Borok village, Yaroslavl Region), Cherepovets State University, Borisov Mikhail Yanovich ,   I.D. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences (Borok village, Yaroslavl Region), Cherepovets State University
Published: March 2022

The mercury content in the muscle tissue of roach and perch from the reservoirs of the Vologda region of the Russian Federation varies from 0,001 to 1,57 mg/kg of raw mass. Using the recommendations of the FAO/WHO, the safe amount of local fish in the diet of the population of the Vologda region is calculated: for adults — 309–1989 kg/week, depending on the type; for children — 83–525 g per week, depending on the type of fish and the age of the child. Using the recommendations of the US EPA, the safe amount of local fish in the diet of the population of the Vologda region is calculated: for adults— 182–350 kg/week, depending on the type; for children— 42–129 g per week, depending on the type of fish and the age of the child. The number of predatory fish in the diet of the population should be two times less than peaceful fish. It was found that the mercury content exceeds the normative levels in place in the Russian Federation (≥ 0,3 μg/g and ≥ 0,6 μg/g, respectively) in 6 % of the fish from non-predatory species and 8 % from predatory fish species. For children of school and preschool age, the proportion of perch that is recommended to be excluded from consumption is 60 and 51 %, respectively; the share of roach is 32 and 18 %, respectively. For the health of the adult population, it may be unsafe to eat more than 30 % of the studied perch and 4 % of the studied roach. English version of the article is available at URL: https://panor.ru/articles/calculation-of-mercury-intake-from-the-consumption-of-wild-fish-by-the-population-of-the-vologda-region/80552.html
Demographic situation and average purchasing power
Authors:   FBSI Federal Scientific Center for Hygiene named after F. F. Erisman of Rospotrebnadzor, Pavlovskaya Nadezhda Alekseevna
Published: March 2022

The study of the patterns of demographic processes in the Russian Federation and the preservation of the people is of crucial importance for the further development of the country. Currently, the number of the indigenous population of Russia is decreasing. The article summarizes the research results of an outstanding scientist, specialist in the field of occupational medicine, academician of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor. Velichkovsky B.T., who identified the regularities of the relationship of a number of economic and medical factors with the intensity of the population decline of the Russian Federation. Scientists have established a connection between the population decline and the value of its purchasing power, the availability of medical personnel, dustiness of the air, living conditions and other factors. It is shown that the most significant role in mortality is the value of the purchasing power of the population. The main diseases leading to an increase in mortality have been identified. The calculations of the author of the article showed that a close relationship between the purchasing power and the mortality rate of the population of the Russian Federation remains at the present time.
Infraestructura tecnológica en universidades públicas como base para la vinculación con la industria automotriz: el caso de la zona metropolitana de San Luis Potosí
Authors: Neri Guzmán Juan Carlos
Published: March 2022

Esta investigación analiza la relación que existe entre las capacidades tecnológicas instaladas en las instituciones públicas de educación superior y las existentes en las empresas pertenecientes a la industria automotriz localizadas en la zona metropolitana de San Luis Potosí, como un punto de referencia para promover la vinculación y la realización de actividades de formación, investigación y transferencia tecnológica. En el estudio, se diseñó un instrumento que permite registrar las 148 tecnologías (hardware y software) características en la industria automotriz, las cuales se agruparon en 11 tipos y en tres niveles de madurez tecnológica (Chui et al., 2017) para evaluar cinco instituciones de educación superior y 28 empresas del sector. Los resultados muestran que la principal diferencia está en el peso que tienen las tecnologías tipo alta-óptima (robótica y celdas de manufactura) en las empresas respecto a las universidades (29.7% contra 9.4%, respectivamente). Asimismo, se identificó que existe un interés por parte de las empresas por llevar a cabo actividades de investigación y capacitación con las universidades (18% y 28.6%, respectivamente). Este estudio aporta a las investigaciones sobre las capacidades tecnológicas locales, pues no existen análisis a este nivel de detalle y son necesarios para orientar la política tecnológica del país y redefinir el papel de las instituciones de educación superior para coadyuvar en la mejora de la competitividad de la región.
In Situ
Investigation of the Phase Transition at the Surface of Thermoelectric PbTe with van der Waals Control
Authors: Cheng Feng , Li Ao , Wang Siliang , Lin Yangjian , Nan Pengfei , Wang Shuai , Cheng Ningyan , Yue Yang , Ge Binghui
Published: March 2022

The structure of thermoelectric materials largely determines the thermoelectric characteristics. Hence, a better understanding of the details of the structural transformation process/conditions can open doors for new applications. In this study, the structural transformation of PbTe (a typical thermoelectric material) is studied at the atomic scale, and both nucleation and growth are analyzed. We found that the phase transition mainly occurs at the surface of the material, and it is mainly determined by the surface energy and the degree of freedom the atoms have. After exposure to an electron beam and high temperature, high-density crystal-nuclei appear on the surface, which continue to grow into large particles. The particle formation is consistent with the known oriented-attachment growth mode. In addition, the geometric structure changes during the transformation process. The growth of nanoparticles is largely determined by the van der Waals force, due to which adjacent particles gradually move closer. During this movement, as the relative position of the particles changes, the direction of the interaction force changes too, which causes the particles to rotate by a certain angle.
No Singular (2012) de Henrique Rodovalho
Authors: Mazza Samuel , Freire Ramos Alcides
Published: March 2022

A partir das notícias referentes ao espetáculo No Singular (2012), da Quasar Cia. De Dança e de algumas críticas publicadas pela pesquisadora Helena Katz sobre outros espetáculos da companhia, iremos tentar estabelecer algumas estratégias desenvolvidas pelo coreógrafo Henrique Rodovalho. O termo “estratégia” é utilizado no livro A Herança Imaterial (1985) de Giovanni Levi, e a partir dessa obra podemos estabelecer um diálogo entre o autor e Jacques Revel (2010) para entendermos melhor as escolhas de Henrique Rodovalho dentro do cenário artístico goiano.