bluuebunny/crossref_metadata_2025 huggingface dataset:
Teladorsagia circumcincta 1,6-Bisphosphate Aldolase: Molecular and Biochemical Characterisation, Structure Analysis and Recognition by Immune Hosts
Authors: Umair Saleh , Bouchet Charlotte , Palevich Nikola , Simpson Heather
Published: January 2021

A 1095 bp full length cDNA encoding Teladorsagia circumcincta aldolase (TciALDO-1) was cloned and expressed in Escherichia coli. Recombinant TciALDO-1 was purified, and its kinetic properties determined. The predicted protein consisted of 365 amino acids, and was present as a single band of about 44 kDa on SDS-PAGE. Multiple alignments of the protein sequence of TciALDO-1 with homologues from other helminths showed the greatest similarity (93%) to the aldolases of Haemonchus contortus and Dictyocaulus viviparus, 82–86% similarity to the other nematode sequences, and 68–71% similarity to cestode and trematode enzymes. Substrate binding sites and conserved regions were identified, and were completely conserved in other homologues. At 30 °C, the optimum pH for TciALDO-1 activity was pH 7.5, the Vmax was 432 ± 23 nmol × min−1 × mg−1 protein, and the apparent Km for the substrate fructose 1,6-bisphosphate was 0.24 ± 0.01 µM (mean ± SEM, n = 3). Recombinant TciALDO-1 was recognized by antibodies in both serum and saliva from field-immune sheep in ELISA, however, that was not the case with nematode-naïve sheep. Teladorsagia circumcincta fructose 1,6-bisphosphate aldolase appears to have potential as a vaccine candidate to control this common sheep parasite.
HPMA-Based Copolymers Carrying STAT3 Inhibitor Cucurbitacin-D as Stimulus-Sensitive Nanomedicines for Oncotherapy
Authors: Tavares Marina R. , Hrabánková Klára , Konefał Rafał , Kaňa Martin , Říhová Blanka , Etrych Tomáš , Šírová Milada , Chytil Petr
Published: January 2021

The study describes the synthesis, physicochemical properties, and biological evaluation of polymer therapeutics based on N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers intended for a tumor-targeted immuno-oncotherapy. Water-soluble linear and cholesterol-containing HPMA precursors were synthesized using controlled reversible addition–fragmentation chain transfer polymerization to reach molecular weight Mn about 2 × 104 g·mol−1 and low dispersity. These linear or self-assembled micellar conjugates, containing immunomodulatory agent cucurbitacin-D (CuD) or the anticancer drug doxorubicin (Dox) covalently bound by the hydrolytically degradable hydrazone bond, showed a hydrodynamic size of 10–30 nm in aqueous solutions. The CuD-containing conjugates were stable in conditions mimicking blood. Importantly, a massive release of active CuD in buffer mimicking the acidic tumor environment was observed. In vitro, both the linear (LP-CuD) and the micellar (MP-CuD) conjugates carrying CuD showed cytostatic/cytotoxic activity against several cancer cell lines. In a murine metastatic and difficult-to-treat 4T1 mammary carcinoma, only LP-CuD showed an anticancer effect. Indeed, the co-treatment with Dox-containing micellar polymer conjugate and LP-CuD showed potentiation of the anticancer effect. The results indicate that the binding of CuD, characterized by prominent hydrophobic nature and low bioavailability, to the polymer carrier allows a safe and effective delivery. Therefore, the conjugate could serve as a potential component of immuno-oncotherapy schemes within the next preclinical evaluation.
Rapid and Specific Detection of the Poplar Black Spot Disease Caused by Marssonina brunnea Using Loop-Mediated Isothermal Amplification Assay
Authors: Xiong Qin , Zhang Linlin , Zheng Xinyue , Qian Yulin , Zhang Yaxin , Zhao Lijuan , Cheng Qiang
Published: January 2021

Marssonina brunnea is the main pathogen that causes poplar black spot disease, which leads to the decrease of the photosynthetic efficiency and significantly affects the production and quality of timber. Currently, no in-field diagnostic exists for M. brunnea. Here, we described a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of M. brunnea. A set of six oligonucleotide primers was designed to recognize eight distinct sequences of the internal transcribed spacer (ITS) region of M. brunnea. The LAMP assay was optimized by the combination of high specificity, sensitivity, and rapidity for the detection of less than 10 pg/μL of target genomic DNA in 60 min per reaction at 65 °C, whereas with PCR, there was no amplification of DNA with concentration less than 1 ng/μL. Among the genomic DNA of 20 fungalisolates, only the samples containing the genomic DNA of M. brunnea changed from violet to sky blue (visible to the naked eye) by using hydroxynaphthol blue (HNB) dye. No DNA was amplified from the eight other fungus species, including two other Marssonina pathogens, three other foliar fungi pathogens of poplar, and three common foliar fungal endophytes of poplar. Moreover, the detection rates of M. brunnea from artificially and naturally infected poplar leaves were 10/16 (62.5%) and 6/16 (37.5%) using PCR, respectively, while the positive-sample ratios were both 16/16 (100%) using the LAMP assay. Overall, the ITS LAMP assay established here can be a better alternative to PCR-based techniques for the specific and sensitive detection of M. brunnea in poplar endemic areas with resource-limited settings.
Comparison of Plant Morphology, Yield and Nutritional Quality of Fagopyrum esculentum and Fagopyrum tataricum Grown under Field Conditions in Belgium
Authors: Aubert Lauranne , Decamps Christian , Jacquemin Guillaume , Quinet Muriel
Published: January 2021

Buckwheat is a pseudocereal with high nutritional and nutraceutical properties. Although common buckwheat (Fagopyrum esculentum) is the main cultivated species, Tartary buckwheat (Fagopyrum tataricum) is gaining interest. In this paper, we compared plant growth, yield-related parameters and seed nutritional qualities of two varieties of F. esculentum and F. tataricum under field conditions in Belgium. Fagopyrum esculentum flowered earlier, produced less nodes, less branches, less inflorescences, but more flowers per inflorescence than F. tataricum. The yield was higher in F. tataricum, while the thousand-grain weight was higher in F. esculentum. Yield ranged between 2037 kg/ha and 3667 kg/ha depending on the species and year. Regarding nutritional qualities, seeds of F. esculentum contained more proteins (15.4% vs. 12.8%) than seeds of F. tataricum although their amino acid profile was similar. Seeds of F. esculentum contained also more Mg (1.36 vs. 1.15 mg/g dry weight (DW)) and less Fe (22.9 vs. 32.6 µg/g DW) and Zn (19.6 vs. 24.5 µg/g DW) than F. tataricum. The main difference between seed nutritional quality was the concentration of flavonoids that was 60 times higher in F. tataricum than in F. esculentum. Both species grow well under Belgian conditions and showed good seed quality.
Preparation and Characterization of a New Polymeric Multi-Layered Material Based K-Carrageenan and Alginate for Efficient Bio-Sorption of Methylene Blue Dye
Authors: Ammar Chiraz , Alminderej Fahad M. , EL-Ghoul Yassine , Jabli Mahjoub , Shafiquzzaman Md.
Published: January 2021

The current study highlights a novel bio-sorbent design based on polyelectrolyte multi-layers (PEM) biopolymeric material. First layer was composed of sodium alginate and the second was constituted of citric acid and k-carrageenan. The PEM system was crosslinked to non-woven cellulosic textile material. Resulting materials were characterized using FT-IR, SEM, and thermal analysis (TGA and DTA). FT-IR analysis confirmed chemical interconnection of PEM bio-sorbent system. SEM features indicated that the microspaces between fibers were filled with layers of functionalizing polymers. PEM exhibited higher surface roughness compared to virgin sample. This modification of the surface morphology confirmed the stability and the effectiveness of the grafting method. Virgin cellulosic sample decomposed at 370 °C. However, PEM samples decomposed at 250 °C and 370 °C, which were attributed to the thermal decomposition of crosslinked sodium alginate and k-carrageenan and cellulose, respectively. The bio-sorbent performances were evaluated under different experimental conditions including pH, time, temperature, and initial dye concentration. The maximum adsorbed amounts of methylene blue are 124.4 mg/g and 522.4 mg/g for the untreated and grafted materials, respectively. The improvement in dye sorption evidenced the grafting of carboxylate and sulfonate groups onto cellulose surface. Adsorption process complied well with pseudo-first-order and Langmuir equations.
Carbon Based Polymeric Nanocomposites for Dye Adsorption: Synthesis, Characterization, and Application
Authors: Ali Khan Moonis , Govindasamy Ramendhirran , Ahmad Akil , Siddiqui Masoom Raza , Alshareef Shareefa Ahmed , Hakami Afnan Ali Hussain , Rafatullah Mohd
Published: January 2021

Agglomeration and restacking can reduce graphene oxide (GO) activity in a wide range of applications. Herein, GO was synthesized by a modified Hummer’s method. To minimize restacking and agglomeration, in situ chemical oxidation polymerization was carried out to embed polyaniline (PANI) chains at the edges of GO sheets, to obtain GO-PANI nanocomposite. The GO-PANI was tested for the adsorptive removal of brilliant green (BG) from an aqueous solution through batch mode studies. Infrared (FT-IR) analysis revealed the dominance of hydroxyl and carboxylic functionalities over the GO-PANI surface. Solution pH-dependent BG uptake was observed, with maximum adsorption at pH 7, and attaining equilibrium in 30 min. The adsorption of BG onto GO-PANI was fit to the Langmuir isotherm, and pseudo-second-order kinetic models, with a maximum monolayer adsorption capacity (qm) of 142.8 mg/g. An endothermic adsorption process was observed. Mechanistically, π-π stacking interaction and electrostatic interaction played a critical role during BG adsorption on GO-PANI.
Influence of Graphene Oxide Contents on Mechanical Behavior of Polyurethane Composites Fabricated with Different Diisocyanates
Authors: Akram Nadia , Saeed Muhammad , Usman Muhammad , Mansha Asim , Anjum Fozia , Zia Khalid Mahmood , Mahmood Irfan , Mumtaz Nida , Gul Khan Waheed
Published: January 2021

The exceptional behavior of graphene has not yet been entirely implicit in the polymer matrix. To explore this fact in the present work, two series of Polyurethan (PU) composites were synthesized. The structural modification was observed by the use of two different diisocyanate of methylene diisocyanate (MDI) and hexamethylene diisocyanate (HMDI) in hydroxylterminated polybutadiene (HTPB) by using I,4 Butane diol (BD) as the chain extender. The variation in hard segment up to 25 (wt.%) in both series led to significant changes in the mechanical behavior of graphene oxide (GO) induced composites. Both series were prepared by an in situ polymerization process. Fourier transform infrared (FTIR) analysis showed a peak in the region of 1700 cm−1, which confirmed the conversion of the NCO group into urethane linkages. Thermal gravimetric analysis (TGA) revealed a thermal stability up to 450 °C @ 90% weight loss. The swelling behavior showed the optimum uptake of 30% of water and 40% of dimethyl sulfoxide (DMSO) with aliphatic diisocyanate. The values of storage modulus (E′), complex modulus (E*), and compliance complex (D*) were observed up to 7 MPa, 8 Mpa, and 0.7 MPa−1, respectively. The degree of entanglement (N) values were calculated from DMA and were found in the range of 1.7 × 10−4 (mol/m3). Phase segregation of PU was observed by scanning electron microscopy (SEM), elucidating the morphology of composites.
Centrality of Religiosity among Select LGBTQs in the Philippines
Authors: del Castillo Fides , del Castillo Clarence Darro , Ching Gregory , Campos Michael Sepidoza
Published: January 2021

This paper investigates the salience of religion and the centrality of religiosity among select LGBTQs. Much consideration has been given to the identity categories of sex, gender, sexual orientation, and religion. Means (M) and standard deviations (SD) were calculated for the overall CRSi-20 score and its five subscales. The results show that the overall CRSi-20 score is 3.68 (SD = 0.89), which indicates that the select LGBTQs are “religious”. As for the core dimensions of religiosity, the ideology subscale received the highest mean score (M = 4.16, SD = 0.88) while the public practice subscale received the lowest mean score (M = 3.21, SD = 1.15). The overall reliability of the survey is computed at 0.96, while the rest of the subscales have alpha values ranging from 0.81 to 0.95. Study outcomes confirm the general religiosity of participants, particularly among older respondents. Of the five subscales, ideology and private practice emerge as dominant categories. In terms of sex distribution, men tend to self-describe as “highly religious” in relation to women, who identify largely as “religious”.
A Tunable and Electrically Small Antenna for Compact GNSS Receivers
Authors: Yashchyshyn Yevhen , Vynnyk Dmytro , Haiduchok Volodymyr , Solskii Ivan , Wu Changying , Bogdan Grzegorz , Modelski Józef
Published: January 2021

The electronic receivers of global navigation satellite systems (GNSSs) are implemented in various handheld electronic devices such as laptops, smartphones, and smartwatches; therefore, their dimensions are of critical importance. Achieving a GNSS terminal of a small size is difficult due to its relatively low operational frequency (L-band), which is equivalent to a wavelength of approximately 24 cm. As an efficient half-wavelength antenna is too large for compact devices, in this paper, an electrically small antenna (ESA) for GNSS terminals is presented. The antenna was miniaturized by using a dielectric block with relatively high permittivity, making some parts virtual, and optimizing its geometry. The operational frequency of the ESA is tunable by means of metallic rods of variable heights inserted into a cylindrical cavity drilled inside the dielectric block. The results confirm the feasibility of the concept and the usability of the ESA for compact GNSS terminals.
Forest Height Estimation Using a Single-Pass Airborne L-Band Polarimetric and Interferometric SAR System and Tomographic Techniques
Authors: Huang Yue , Zhang Qiaoping , Ferro-Famil Laurent
Published: January 2021

This paper addresses forest height estimation for boreal forests at the test site of Edson in Alberta, Canada, using dual-baseline PolInSAR dataset measured by Intermap’s single-pass system. This particular dataset is acquired by using both ping-pong and non-ping-pong modes, which permit forming a dual-baseline TomoSAR configuration, i.e., an extreme configuration for tomographic processing. A tomographic approach, based on polarimetric Capon and MUSIC estimators, is proposed to estimate the elevation of tree top and of underlying ground, and hence forest height is estimated. The resulting forest DTM and DSM over the test site are validated against LiDAR-derived estimates, demonstrating the undeniable capability of the single-pass L-band PolInSAR system for forest monitoring.
Implementation of an Improved Water Change Tracking (IWCT) Algorithm: Monitoring the Water Changes in Tianjin over 1984–2019 Using Landsat Time-Series Data
Authors: Han Xingxing , Chen Wei , Ping Bo , Hu Yong
Published: January 2021

Tianjin is the largest open city along the coastline in Northern China, which has several important wetland ecosystems. However, no systematic study has assessed the water body changes over the past few decades for Tianjin, not to mention their response to human activities and climate change. Here, based on the water change tracking (WCT) algorithm, we proposed an improved water change tracking (IWCT) algorithm, which could remove built-up shade noise (account for 0.4%~6.0% of the final water area) and correct omitted water pixels (account for 1.1%~5.1% of the final water area) by taking the time-series data into consideration. The seasonal water product of the Global Surface Water Data (GSWD) was used to provide a comparison with the IWCT results. Significant changes in water bodies of the selected area in Tianjin were revealed from the time-series water maps. The permanent water area of Tianjin decreased 282.5 km2 from 1984 to 2019. Each time after the dried-up period, due to government policies, the land reclamation happened in Tuanbo Birds Nature Reserve (TBNR), and, finally, 12.6 km2 of the lake has been reclaimed. Meanwhile, 488.6 km2 of land has been reclaimed from the sea along the coastal zone in the past 16 years at a speed of 28.74 km2 yr−1 in the Binhai New Area (BHNA). The method developed in this study could be extended to other sensors which have similar band settings with Landsat; the products acquired in this study could provide fundamental reference for the wetland management in Tianjin.
Real-Time Rainfall Estimation Using Microwave Links: A Case Study in East China during the Plum Rain Season in 2020
Authors: Song Kun , Liu Xichuan , Gao Taichang
Published: January 2021

Accurate and real-time rainfall estimation is a pressing need for forecasting the flood disaster and reducing the loss. In this study, we exploit the potential of estimating the rainfall by microwave links in East China. Eight microwave links at 15 GHz and 23 GHz, operated by China Mobile, are used for estimating the rain rate in real-time in Jiangyin, China from June to July 2020. First, we analyze the correlation between the rain-induced attenuation of microwave links and the rain rate measured by rain gauges. The correlation coefficient values are higher than 0.77 with the highest one over 0.9, showing a strong positive correlation. The real-time results indicate that microwave links estimate the rainfall with a higher temporal resolution than the rain gauges. Meanwhile, the rain rate that was estimated by microwave links also correlates well with the actual rain rate, and most of the values of the mean absolute error are less than 1.50 mm/h. Besides, the total rainfall’s relative deviation values are less than 5% with the smallest one reaching 1%. The quantitative results also indicate that microwave links could lead to better forecasting of water levels and, hence, better warnings for flood disasters.
Lights and Shadows: A Comprehensive Survey on Cooperative and Precoding Schemes to Overcome LOS Blockage and Interference in Indoor VLC
Authors: Céspedes Máximo Morales , Guzmán Borja Genovés , Jiménez Víctor P. Gil
Published: January 2021

Visible light communications (VLC) have received significant attention as a way of moving part of the saturated indoor wireless traffic to the wide and unregulated visible optical spectrum. Nowadays, VLC are considered as a suitable technology, for several applications such as high-rate data transmission, supporting internet of things communications or positioning. The signal processing originally derived from radio-frequency (RF) systems such as cooperative or precoding schemes can be applied to VLC. However, its implementation is not straightforward. Furthermore, unlike RF transmission, VLC present a predominant line-of-sight link, although a weak non-LoS component may appear due to the reflection of the light on walls, floor, ceiling and nearby objects. Blocking effects may compromise the performance of the aforementioned transmission schemes. There exist several surveys in the literature focused on VLC and its applications, but the management of the shadowing and interference in VLC requires a comprehensive study. To fill this gap, this work introduces the implementation of cooperative and precoding schemes to VLC, while remarking their benefits and drawbacks for overcoming the shadowing effects. After that, the combination of both cooperative and precoding schemes is analyzed as a way of providing resilient VLC networks. Finally, we propose several open issues that the cooperative and precoding schemes must face in order to provide satisfactory VLC performance in indoor scenarios.
Influence of Flow Disturbances behind the 90° Bend on the Indications of the Ultrasonic Flow Meter with Clamp-On Sensors on Pipelines
Authors: Synowiec Piotr , Andruszkiewicz Artur , Wędrychowicz Wiesław , Piechota Piotr , Wróblewska Elżbieta
Published: January 2021

The subject matter of the article concerns velocities/flow rate measurements in the area of disturbed flows-behind the 90° bend. They were conducted by means of an ultrasonic flowmeter with clamp-on sensors on pipeline, for water and two different Reynolds numbers of 70,000 and 100,000, corresponding to two velocities of approximately 1.42 m/s and 2.04 m/s. The tests were carried out at 12 distances from the disturbance. Sensors on the circumference of the pipeline were mounted 30° each. The correction factor values were calculated for the given measurement geometry. The measurements have shown that the values of this coefficient are always greater than 1, which means that the ultrasonic flow meter understates the speed values. They also showed that already at a distance of 8 nominal diameters from the disturbance, the correction factor does not exceed 1.02, so the measurement errors are within the maximum permissible error (MPE) of a typical ultrasonic flow meter. For distances less than eight nominal diameters from the disturbance, not taking the correction factor value into the account can lead to systematic errors of up to 10.8%. Studies have also proved that in each measurement plane behind the disturbance there are two mounting angles for the ultrasonic sensors, 60° and 240° respectively, for which the correction factor values are minimal. Additionally, using the laser Doppler anemometry (LDA) method, velocity solids were determined at individual distances from the disturbance, and the projections of velocity blocks on the appropriate plane represented velocity profiles and indicated the distances from the disturbance at which these profiles stabilise.
A Compact Multi-Distance DCS and Time Domain NIRS Hybrid System for Hemodynamic and Metabolic Measurements
Authors: Amendola Caterina , Lacerenza Michele , Buttafava Mauro , Tosi Alberto , Spinelli Lorenzo , Contini Davide , Torricelli Alessandro
Published: January 2021

In this work, we present a new multi-distance diffuse correlation spectroscopy (DCS) device integrated with a compact state-of-the-art time domain near infrared spectroscopy (TD-NIRS) device. The hybrid DCS and TD-NIRS system allows to retrieve information on blood flow, tissue oxygenation, and oxygen metabolic rate. The DCS device performances were estimated in terms of stability, repeatability, ability in retrieving variations of diffusion coefficient, influence of the tissue optical properties, effect of varying count rates and depth sensitivity. Crosstalk between DCS and TD-NIRS optical signals was also evaluated. Finally, in vivo experiments (venous and arterial cuff occlusions on the arm) were conducted to test the ability of the hybrid system in measuring blood flow variations.
Soil Moisture Retrieval in Farmland Areas with Sentinel Multi-Source Data Based on Regression Convolutional Neural Networks
Authors: Liu Jian , Xu Youshuan , Li Henghui , Guo Jiao
Published: January 2021

As an important component of the earth ecosystem, soil moisture monitoring is of great significance in the fields of crop growth monitoring, crop yield estimation, variable irrigation, and other related applications. In order to mitigate or eliminate the impacts of sparse vegetation covers in farmland areas, this study combines multi-source remote sensing data from Sentinel-1 radar and Sentinel-2 optical satellites to quantitatively retrieve soil moisture content. Firstly, a traditional Oh model was applied to estimate soil moisture content after removing vegetation influence by a water cloud model. Secondly, support vector regression (SVR) and generalized regression neural network (GRNN) models were used to establish the relationships between various remote sensing features and real soil moisture. Finally, a regression convolutional neural network (CNNR) model is constructed to extract deep-level features of remote sensing data to increase soil moisture retrieval accuracy. In addition, polarimetric decomposition features for real Sentinel-1 PolSAR data are also included in the construction of inversion models. Based on the established soil moisture retrieval models, this study analyzes the influence of each input feature on the inversion accuracy in detail. The experimental results show that the optimal combination of R2 and root mean square error (RMSE) for SVR is 0.7619 and 0.0257 cm3/cm3, respectively. The optimal combination of R2 and RMSE for GRNN is 0.7098 and 0.0264 cm3/cm3, respectively. Especially, the CNNR model with optimal feature combination can generate inversion results with the highest accuracy, whose R2 and RMSE reach up to 0.8947 and 0.0208 cm3/cm3, respectively. Compared to other methods, the proposed algorithm improves the accuracy of soil moisture retrieval from synthetic aperture radar (SAR) and optical data. Furthermore, after adding polarization decomposition features, the R2 of CNNR is raised by 0.1524 and the RMSE of CNNR decreased by 0.0019 cm3/cm3 on average, which means that the addition of polarimetric decomposition features effectively improves the accuracy of soil moisture retrieval results.
Remote Monitoring in Chronic Heart Failure Patients: Is Non-Invasive Remote Monitoring the Way to Go?
Authors: Veenis Jesse F. , Radhoe Sumant P. , Hooijmans Petra , Brugts Jasper J.
Published: January 2021

Heart failure (HF) is a major health care issue, and the incidence of HF is only expected to grow further. Due to the frequent hospitalizations, HF places a major burden on the available hospital and healthcare resources. In the future, HF care should not only be organized solely at the clinical ward and outpatient clinics, but remote monitoring strategies are urgently needed to guide, monitor, and treat chronic HF patients remotely from their homes as well. The intuitiveness and relatively low costs of non-invasive remote monitoring tools make them an appealing and emerging concept for developing new medical apps and devices. The recent COVID-19 pandemic and the associated transition of patient care outside the hospital will boost the development of remote monitoring tools, and many strategies will be reinvented with modern tools. However, it is important to look carefully at the inconsistencies that have been reported in non-invasive remote monitoring effectiveness. With this review, we provide an up-to-date overview of the available evidence on non-invasive remote monitoring in chronic HF patients and provide future perspectives that may significantly benefit the broader group of HF patients.
Robust Visual Tracking with Reliable Object Information and Kalman Filter
Authors: Chen Hang , Zhang Weiguo , Yan Danghui
Published: January 2021

Object information significantly affects the performance of visual tracking. However, it is difficult to obtain accurate target foreground information because of the existence of challenging scenarios, such as occlusion, background clutter, drastic change of appearance, and so forth. Traditional correlation filter methods roughly use linear interpolation to update the model, which may lead to the introduction of noise and the loss of reliable target information, resulting in the degradation of tracking performance. In this paper, we propose a novel robust visual tracking framework with reliable object information and Kalman filter (KF). Firstly, we analyze the reliability of the tracking process, calculate the confidence of the target information at the current estimated location, and determine whether it is necessary to carry out the online training and update step. Secondly, we also model the target motion between frames with a KF module, and use it to supplement the correlation filter estimation. Finally, in order to keep the most reliable target information of the first frame in the whole tracking process, we propose a new online training method, which can improve the robustness of the tracker. Extensive experiments on several benchmarks demonstrate the effectiveness and robustness of our proposed method, and our method achieves a comparable or better performance compared with several other state-of-the-art trackers.
Citizenship Education and Civil Society
Authors: Kisby Ben
Published: January 2021

Contemporary societies face a range of important challenges, including: climate change; poverty; wealth, income, and other forms of social inequality; human rights abuses; misinformation and fake news; the growth of populist movements; and citizen disenchantment with democratic politics [...]
Impact of Climate Change Adaptation on Household Food Security in Nigeria—A Difference-in-Difference Approach
Authors: Ogunpaimo Oyinlola Rafiat , Oyetunde-Usman Zainab , Surajudeen Jolaosho
Published: January 2021

Studies have shown that climate change adaptation options (CCA) are implemented to buffer the unfavorable climatic changes in Nigeria causing a decline in food security. Against the background of measuring the impact of CCA options using cross-sectional data, this study assessed how CCA had affected food security using panel data on farming households from 2010–2016 obtained from Nigerian General Household Survey (GHS). Data were analyzed using the Panel probit model (PPM), Propensity Score Matching (PSM), and Difference-in-Difference (DID) regression. PPM showed that the probability of adopting CCA options increased with farm size (p < 0.01), extension contact (p < 0.01), and marital status (p < 0.01), but decreased with the age of the household head (p < 0.01). Credit facilities (p < 0.05), ownership of farmland (p < 0.01), household size (p < 0.01), years of schooling (p < 0.01), household asset (p < 0.01), and location (p < 0.05) also had a significant but mixed effect on CCA choices. PSM revealed that farming households that adopted CCA strategies had 9% higher food security levels than non-adopters. Furthermore, the result of the DID model revealed a significant positive effect of CCA on household food security (β = 5.93, p < 0.01). It was recommended that education and provision of quality advisory services to farmers is crucial to foster the implementation of CCA options.
The Carmine Maggiore Bell Tower: An Inclusive and Sustainable Restoration Experience
Authors: Nuzzo Mariano , Faella Giuseppe
Published: January 2021

The preservation of cultural heritage needs a restoration design format that can only be achievable by an in-depth multidisciplinary approach. Besides, a sustainable project requires that the link between architecture and technology be expressed through a “conscious” approach to the building. Therefore, each design must be based on the in-depth knowledge of history, construction rules, and mechanical properties of buildings to be restored. The bell towers are among the most exposed to degradation and earthquake damage constructions for their intrinsic geometry and structure. The “Carmine Maggiore” bell tower is one of the most important symbols of Naples (Italy) and, at 72 m high, it has stood out for centuries against the city. The tower underwent many significant damages and structural changes over time. The design, here illustrated, was aimed at restoring and repairing this bell tower, preserving existing materials, without altering the signs of time. The paper shows the methodology followed in the project, which, starting from an in-depth historical analysis and a detailed geometric and diagnostic survey, through several structural analyses, allowed an “inclusive” project (restoration, structural, and lighting project) based on suitable solutions that meet all the requirements of compatibility, sustainability, and structural safety.
Analysis of Inlet Configurations on the Microclimate Conditions of a Novel Standalone Agricultural Greenhouse for Egypt Using Computational Fluid Dynamics
Authors: Akrami Mohammad , Mutlum Can Dogan , Javadi Akbar A. , Salah Alaa H. , Fath Hassan E. S. , Dibaj Mahdieh , Farmani Raziyeh , Mohammed Ramy H. , Negm Abdelazim
Published: January 2021

Water shortage, human population increase, and lack of food resources have directed societies towards sustainable energy and water resources, especially for agriculture. While open agriculture requires a massive amount of water and energy, the requirements of horticultural systems can be controlled to provide standard conditions for the plants to grow, with significant decrease in water consumption. A greenhouse is a transparent indoor environment used for horticulture, as it allows for reasonable control of the microclimate conditions (e.g., temperature, air velocity, rate of ventilation, and humidity). While such systems create a controlled environment for the plants, the greenhouses need ventilation to provide fresh air. In order to have a sustainable venting mechanism, a novel solution has been proposed in this study providing a naturally ventilating system required for the plants, while at the same time reducing the energy requirements for cooling or other forced ventilation techniques. Computational fluid dynamics (CFD) was used to analyse the ventilation requirements for different vent opening scenarios, showing the importance of inlet locations for the proposed sustainable greenhouse system.
Evaluating Walkability through a Multi-Criteria Decision Analysis Approach: A Lisbon Case Study
Authors: Manzolli Jônatas Augusto , Oliveira André , Neto Miguel de Castro
Published: January 2021

New strategies to improve the quality of urban pedestrian environments are becoming increasingly important in sustainable city planning. This trend has been driven by the advantages that active mobility provides in terms of health, social, and environmental aspects. Our work explores the idea of walkability. This concept refers to the friendliness of the urban environment to pedestrian traffic. We propose a framework based on the multi-criteria decision analysis (MCDA) methodology to rank streets in terms of walkability levels. The city of Lisbon (Portugal) is the location of the streets under examination. Findings confirmed the framework’s replicability and suggested the possibility of this strategy being used as a support tool for designing urban policies.
Municipal Solid Waste Characterization and Landfill Gas Generation in Kakia Landfill, Makkah
Authors: Osra Faisal A. , Ozcan Huseyin Kurtulus , Alzahrani Jaber S. , Alsoufi Mohammad S.
Published: January 2021

In many countries, open dumping is considered the simplest, cheapest, and most cost-effective way of managing solid wastes. Thus, in underdeveloped economies, Municipal Solid Wastes (MSW) are openly dumped. Improper waste disposal causes air, water, and soil pollution, impairing soil permeability and blockage of the drainage system. Solid Waste Management (SWM) can be enhanced by operating a well-engineered site with the capacity to reduce, reuse, and recover MSW. Makkah city is one of the holiest cities in the world. It harbors a dozen of holy places. Millions of people across the globe visit the place every year to perform Hajj, Umrah, and tourism. In the present study, MSW characterization and energy recovery from MSW of Makkah was determined. The average composition of solid waste in Makkah city is organic matter (48%), plastics (25%), paper and cardboard (20%), metals (4%), glass (2%), textiles (1%), and wood (1%). In order to evaluate energy recovery potential from solid waste in Kakia open dumpsite landfill, the Gas Generation Model (LandGEM) was used. According to LandGEM results, landfill gas (methane and carbon dioxide) generation potential and capacity were determined. Kakia open dump has a methane potential of 83.52 m3 per ton of waste.
Contact Symmetries of a Model in Optimal Investment Theory
Authors: Arrigo Daniel J. , Van de Grift Joseph A.
Published: January 2021

It is generally known that Lie symmetries of differential equations can lead to a reduction of the governing equation(s), lead to exact solutions of these equations and, in the best case scenario, lead to a linearization of the original equation. In this paper, we consider a model from optimal investment theory where we show the governing equation possesses an extensive contact symmetry and, through this, we show it is linearizable. Several exact solutions are provided including a solution to a particular terminal value problem.
The Enhanced-Earned Value Management (E-EVM) Model: A Proposal for the Aerospace Industry
Authors: López Pascual Joaquín , Meléndez Rodríguez Juan Carlos , Cruz Rambaud Salvador
Published: January 2021

The framework of this paper is the aerospace industry, which is one of the world’s leading sectors, thus playing a noteworthy role in current society. This makes it especially important to try to optimize the management of the aerospace sector. Therefore, the main objective of this paper is to propose the so-called Enhanced-Earned Value Management (hereinafter, E-EVM) model, able to explore the simultaneous evaluation of many projects, from which the management of the project can take advantage. Additionally, this model considers the possibility of forecasting pending tasks, measurable in time units or cost units, until the end of the project. The main contribution of E-EVM methodology is its capacity to detect both delayed and advanced projects by converting times (hours) into monetary units (EUR). Empirically, this enhanced model has been applied to a real case study in the aerospace industry composed of thousands of subprojects and the results provide the project manager with valuable information to make decisions in a short term. Through computer graphic representation techniques, the visualization of project deployment can be improved. Finally, the E-EVM model can be used even in big projects where a very large volume of information must be simultaneously treated and also, it will be suitable to apply pattern recognition concerning the project performance.
Antiviral Activity of the G-Quadruplex Ligand TMPyP4 against Herpes Simplex Virus-1
Authors: Artusi Sara , Ruggiero Emanuela , Nadai Matteo , Tosoni Beatrice , Perrone Rosalba , Ferino Annalisa , Zanin Irene , Xodo Luigi , Flamand Louis , Richter Sara N.
Published: January 2021

The herpes simplex virus 1 (HSV-1) genome is extremely rich in guanine tracts that fold into G-quadruplexes (G4s), nucleic acid secondary structures implicated in key biological functions. Viral G4s were visualized in HSV-1 infected cells, with massive virus cycle-dependent G4-formation peaking during viral DNA replication. Small molecules that specifically interact with G4s have been shown to inhibit HSV-1 DNA replication. We here investigated the antiviral activity of TMPyP4, a porphyrin known to interact with G4s. The analogue TMPyP2, with lower G4 affinity, was used as control. We showed by biophysical analysis that TMPyP4 interacts with HSV-1 G4s, and inhibits polymerase progression in vitro; in infected cells, it displayed good antiviral activity which, however, was independent of inhibition of virus DNA replication or entry. At low TMPyP4 concentration, the virus released by the cells was almost null, while inside the cell virus amounts were at control levels. TEM analysis showed that virus particles were trapped inside cytoplasmatic vesicles, which could not be ascribed to autophagy, as proven by RT-qPCR, western blot, and immunofluorescence analysis. Our data indicate a unique mechanism of action of TMPyP4 against HSV-1, and suggest the unprecedented involvement of currently unknown G4s in viral or antiviral cellular defense pathways.
New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution
Authors: Frétaud Maxence , Descamps Delphyne , Laubreton Daphné , Rameix-Welti Marie-Anne , Eléouët Jean-François , Larcher Thibaut , Galloux Marie , Langevin Christelle
Published: January 2021

Background: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiology of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus–host interactions and related acute respiratory disease, at the level of the entire lung. Methods: By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Results: Whole tissue analysis allowed the identification of infected cell subtypes, based on both morphological traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Conclusions: Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiology and improve the 2D histology analyses.
Multiple Sclerosis and SARS-CoV-2 Vaccination: Considerations for Immune-Depleting Therapies
Authors: Sellner Johann , Rommer Paulus S.
Published: January 2021

Several concerns have been raised about the use of immunodepleting agents including alemtuzumab, cladribine and CD20-depleting antibodies in people with multiple sclerosis (pwMS) during the coronavirus disease (COVID) 2019 pandemic. As the end of the pandemic is not yet in sight, vaccination against severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) may be an elegant strategy to overcome the potential hazards associated with initiating and continuing treatment with immune-depleting agents. In this review, we summarize the immunological effects of immune-depleting therapy and underlying considerations for the hitherto existing recommendations that suggest a restricted use of immune-deleting therapies during the pandemic. Moreover, we critically discuss open questions regarding vaccination in general and against SARS-CoV-2 in pwMS.
Effects of Vegetation Restoration on Regional Soil Moisture Content in the Humid Karst Areas—A Case Study of Southwest China
Authors: Wei Xiaocha , Zhou Qiuwen , Cai Mingyong , Wang Yujuan
Published: January 2021

Soil moisture is one of the restricting factors in the humid karst areas, which feature strong spatial heterogeneity. However, current research about the effects of vegetation restoration on soil moisture content have mainly focused on plot scale and slope scale, while these effects still remain unclear at regional scale in this area. Taking Southwest China as a case study and based on the land parameter data record (LPDR) and enhanced vegetation index (EVI) data set during 2002–2018, this study analyzed the spatiotemporal variation characteristics of vegetation and soil moisture content, and evaluated the effects of vegetation restoration on regional soil moisture content dynamics in paired years with similar precipitation conditions. The results showed that the EVI generally increased at a rate of 0.035/10a during 2002–2018, while the soil moisture was dominated by a drying trend at a variation rate of −0.0006 (cm3/cm3)/10a. The increasing trend of EVI accounted for 90.90% across the study area, whereas the decreasing trend of soil moisture accounted for 51.66%, and the increasing trend of soil moisture accounted for 48.34%. In addition, the decreasing trend of soil moisture coupled with an increasing trend of EVI distributed in most of the study area, especially in the homogenous limestone area. Our results demonstrate that there were remarkable vegetation restoration efforts in a series of ecological restoration projects, which resulted in a drying trend of the regional soil moisture content in the humid karst areas. The results suggest that it is necessary to consider reasonable vegetation planting density and suitable revegetation types to balance the relationship between vegetation water consumption and soil moisture supplementation in vegetation restoration practice in the humid karst areas.
Investigate Impact Force of Dam-Break Flow against Structures by Both 2D and 3D Numerical Simulations
Authors: Hien Le Thi Thu , Van Chien Nguyen
Published: January 2021

The aim of this paper was to investigate the ability of some 2D and 3D numerical models to simulate flood waves in the presence of an isolated building or building array in an inundated area. Firstly, the proposed 2D numerical model was based on the finite-volume method (FVM) to solve 2D shallow-water equations (2D-SWEs) on structured mesh. The flux-difference splitting method (FDS) was utilized to obtain an exact mass balance while the Roe scheme was invoked to approximate Riemann problems. Secondly, the 3D commercially available CFD software package was selected, which contained a Flow 3D model with two turbulent models: Reynolds-averaged Navier-Stokes (RANs) with a renormalized group (RNG) and a large-eddy simulation (LES). The numerical results of an impact force on an obstruction due to a dam-break flow showed that a 3D solution was much better than a 2D one. By comparing the 3D numerical force results of an impact force acting on building arrays with the existence experimental data, the influence of velocity-induced force on a dynamic force was quantified by a function of the Froude number and the water depth of the incident wave. Furthermore, we investigated the effect of the initial water stage and dam-break width on the 3D-computed results of the peak value of force intensity.
REGULARITIES OF GROWTH AND FORMATION OF FOREST PLANTATIONS ON HUMUS-CARBONATE SOILS OF THE CENTRAL BLACK EARTH REGION
Authors: Dedenko Tat'yana
Published: January 2021

The problem of afforestation of zonal humus-calcareous soils with close occurrence of chalk rocks has been considered. The research results of reclamation plantations on zonal humus-carbonate soils (Voronezh region, Ostrogozhsky district, Korotoyaksky district forestry) are presented. The main taxation indicators of silver birch (Betula pendula), English oak (Quercus robur), Scots pine (Pinus sylvestris), such as average height and diameter, bonitet, density, preservation, stock, increase in stock, have been found. The characteristics of the current state and productivity of protective plantations are given. The course of growth and the state of Scots pine plantations on humus-carbonate soils depends on the depth of the chalk rocks. Less demanding tree species for soil and soil conditions, such as Betula pendula, Pinus sylvestris, have higher growth rates than more demanding species. It is possible to grow Pinus sylvestris plantations on humus-carbonate soils with a thickness of 40-50 cm - I bonitet; 30-40 cm - II bonitet; 15-20 cm - II - III bonitet. The influence of slabby chalk depth on the coefficient of growth stress is at least 90% of the total amount of influencing factors. With an increase in the thickness of the humus-carbonate layer from 15 - 20 cm to 40 - 50 cm, the growth stress coefficient decreases 2.4-2.5 times. It is not recommended to create plantations with 1.0 × 0.5 m planting points (15-20 thousand units/ha). Such plantations have a very high growth stress coefficient and are biologically weakened