bluuebunny/crossref_metadata_2025 huggingface dataset:
Mass cytometry: exploring the immune landscape of systemic autoimmune and inflammatory diseases in the past fourteen years
Authors: Kante Aïcha , Chevalier Mathieu F. , Sène Damien , Chauffier Jeanne , Mouly Stéphane , Chousterman Benjamin Glenn , Azibani Fériel , Terrier Benjamin , Pezel Théo , Comarmond Cloé
Published: January 2025

Auto-immune and inflammatory diseases are heterogenous in their clinical manifestations and prognosis, even among individuals presenting with the same pathology. Understanding the immunological alterations involved in their pathogenesis provides valuable insights in different clinical phenotypes and treatment responses. Immunophenotyping could lead to significant improvements in diagnosis, monitoring, initial treatment decisions and follow-up in autoimmune and inflammatory diseases. Mass cytometry provides measurement of over 40 simultaneous cellular parameters at single-cell resolution, and therefore holds immense potential to evaluate complex cellular systems and for high-dimensional single-cell analysis. The high dimensionality of mass cytometry provides better coverage of immune populations dynamics, with sufficient power to identify rare cell types compared to flow cytometry. In this comprehensive review, we explore how mass cytometry findings contributed in the past decade to a deeper understanding of the cellular actors involved in systemic auto-immune and auto-inflammatory diseases with their respective therapeutic and prognostic impact. We also delve into the bioinformatical approaches applied to mass cytometry to analyze the high volumes of data generated, as well as the impact of the use of complementary single cell RNA sequencing, and their spatial modalities. Our analysis highlights the fact that mass cytometry captures major information on cell populations providing insights on the complex pathogenesis of autoimmune diseases. Future research designs could include mass cytometry findings in association to other -omics to stratify patients in adequate therapeutic arms and provide advancements in personalized therapies in the field of auto-immune and inflammatory diseases.
Assessing donor kidney function: the role of CIRBP in predicting delayed graft function post-transplant
Authors: Leng Qianghua , Ma Maolin , Tang Zuofu , Jiang Weichen , Han Fei , Huang Zhengyu
Published: January 2025

Introduction

Delayed graft function (DGF) shortens the survival time of transplanted kidneys and increases the risk of rejection. Current methods are inadequate in predicting DGF. More precise tools are required to assess kidney suitability for transplantation. Cold-inducible RNA-binding protein (CIRBP) expression has been linked to acute kidney injury, suggesting its potential as a new biomarker for transplanted kidney function.

Methods

We included deceased donors and recipients who had undergone successful kidney transplantation between 2016 and 2019. Recipients and their paired donors are assigned to either the DGF or immediate graft function (IGF) group, based on the recipient’s recovery of graft renal function. Donor plasma CIRBP levels were measured using an enzyme-linked immunosorbent assay kit to assess their relationships with DGF.

Results

Donor plasma CIRBP concentrations in the DGF group were approximately twice as high as those in the IGF group (6.82 vs. 3.44; P<0.001). DGF occurred in all cases where CIRBP concentrations exceeded 7.92 ng/mL. Furthermore, univariate and multivariate analyses (odds ratio [OR]=1.660; P<0.001) confirmed that donor plasma CIRBP level was an independent risk factor for DGF. Additionally, higher CIRBP levels were associated with increased plasma creatinine at 6 months (R²=0.08; P<0.001), and survival analysis showed shorter kidney survival in recipients with DGF (P=0.002).

Conclusions

This study demonstrated that donor plasma CIRBP levels can effectively predict the occurrence of DGF. CIRBP is a potential novel biomarker for evaluating transplanted kidney function.

Clinical trial registration

https://clinicaltrials.gov, identifier NCT06641622.
Immunological composition of human milk before and during subclinical and clinical mastitis
Authors: Castro-Navarro Irma , Pace Ryan M. , Williams Janet E. , Pace Christina D. W. , Kaur Harpreet , Piaskowski Julia , Aragón Alberto , Rodríguez Juan M. , McGuire Mark A. , Fernandez Leonides , McGuire Michelle K.
Published: January 2025

Mastitis, an inflammatory condition affecting more than 25% of breastfeeding women, is usually associated with reduced milk secretion, pain, and discomfort, which often leads to early cessation of breastfeeding. Although the etiology of mastitis is multifactorial, a pro-inflammatory state of the mammary gland might be a risk factor. However, changes in milk composition, and specifically in the milk immune profile, prior to and during mastitis have not been well described. To help close this research gap, we documented the immune profiles of milk produced by both breasts of 10 women experiencing clinical (CM) and 8 women experiencing subclinical (SCM) mastitis during the week of sign/symptom development as well as the week prior and compared them with milk produced by 14 healthy controls. CM was defined as having signs/symptoms of mastitis, whereas SCM was presumed if the participant did not have signs/symptoms of CM, but her milk had a somatic cell count >400,000 cell/mL and/or sodium-to-potassium (Na/K) ratio >1.0. Concentration of 36 immune factors (including immunoglobulins, cytokines, chemokines, and growth factors) was quantified via immunoassays. Milk produced by women who developed CM had distinct immune profiles the week prior to diagnosis, particularly elevated concentrations of pro-inflammatory cytokine IL-1β and regulatory cytokines IL-2, IL-4 and IL-10. In contrast, immune profiles in milk produced by women with SCM did not differ from that produced by healthy women or those with CM the week prior to mastitis onset. Once mastitis appeared, marked changes in milk’s immune profile were observed in both CM and SCM groups. CM was characterized by elevated concentrations of 27 compounds, including pro-inflammatory cytokines (IL-1β, IL-1ra, and TNFα) and chemokines (including IL-8, eotaxin, IP-10, MCP-1, MIP1α, and MIP1β), compared to healthy controls. Milk’s immune profile during SCM was intermediate, showing higher levels of IL-6, IFNγ, and MCP-1 compared to healthy controls, suggesting a milder, more controlled immune response compared to CM. Only milk produced by the mastitis-affected breast had altered immune profiles. Further research is needed to determine if these differences in milk’s immune profiles can be used to improve mastitis risk prediction prior to onset of symptoms.
New insights into the mechanisms of the immune microenvironment and immunotherapy in osteosarcoma
Authors: Luo Cong , Min Xingxing , Zhang Danying
Published: January 2025

Osteosarcoma, a malignant bone tumor primarily affecting adolescents, is highly invasive with a poor prognosis. While surgery and chemotherapy have improved survival for localized cases, pulmonary metastasis significantly reduces survival to approximately 20%, highlighting the need for novel treatments. Immunotherapy, which leverages the immune system to target osteosarcoma cells, shows promise. This review summarizes the biological characteristics of osteosarcoma, mechanisms of pulmonary metastasis, and the tumor immune microenvironment (TME). It involves recent immunotherapy advances, including monoclonal antibodies, tumor vaccines, immune cell therapies, checkpoint inhibitors, and oncolytic viruses, and discusses combining these with standard treatments.
Construction and validation of a predictive model for the risk of prolonged preoperative waiting time in patients with intertrochanteric fractures
Authors: Gong Rui , Jin Xi-min , Xu Lian-you , Zhang Zhi-meng , Yuan Dao-tong , Xie Wen-peng , Zhang Yong-kui
Published: January 2025

Background

Intertrochanteric fractures are one of the most common types of hip fractures, with delayed surgical treatment beyond 48 h associated with increased postoperative complications and mortality, especially in older adults. This study aimed to develop a predictive model for delayed preoperative waiting times in intertrochanteric fracture cases, based on previous research, to offer a valuable reference for clinical decision-making.

Methods

A retrospective analysis was conducted on 1,116 patients with intertrochanteric fractures admitted to the Affiliated Hospital of Shandong University of Traditional Chinese Medicine for internal fixation surgery from January 2017 to January 2024. Patient demographic data and clinical examination results were collected. A logistic regression model was used to construct a predictive model, which was then visualized through a nomogram. The model’s performance was subsequently validated.

Results

The predictive model developed from 728 patients in the training cohort, identified key predictors, including age, sex, lower extremity deep vein thrombosis, injury location, and biochemical markers. The model demonstrated strong discriminative ability, with an area under the receiver operating characteristic curve of 0.749 (95% confidence interval: 0.621–0.801) for the training set, and 0.745 in the validation set. Calibration curves indicated that the predicted risk of surgical delay closely aligned with observed outcomes. Furthermore, decision curve analysis verified the model’s clinical utility, demonstrating its effectiveness in guiding treatment decisions.

Conclusion

The nomogram model developed in this study provides a reliable tool for predicting delayed surgical intervention in patients with intertrochanteric femur fractures. It offers clinicians a valuable reference to anticipate delays in surgical treatment and aids in the formulation of more timely and appropriate treatment strategies, potentially improving patient outcomes.
Cutaneous pseudolymphoma: a case series of three patients
Authors: Yao Yue , He Yijia , Wu Zhuochen , Zhang Jinfang , Zhang Guoqiang
Published: January 2025

Cutaneous pseudolymphoma is a type of reactive lymphoid hyperplasia that pathologically and/or clinically mimics cutaneous lymphoma. It has a variety of pathogenic factors. However, in many cases, no etiology can be identified. The histopathological findings of cutaneous pseudolymphoma can only be used as the basis for suspected diagnosis. Clinical data, further diagnostic tests and follow-up are needed to confirm the diagnosis. This article reports three cases of cutaneous pseudolymphoma, with different etiology, clinical manifestations and treatment methods. The purpose of this article is to emphasize that the etiology and clinical manifestations of cutaneous pseudolymphoma are diverse, and targeted treatment strategies and long-term follow-up are needed.
The process of nitrogen-adaptation root endophytic bacterial rather than phosphorus-adaptation fungal subcommunities construction unveiled the tomato yield improvement under long-term fertilization
Authors: Li Xiaoxia , Awais Muhammad , Wang Shuang , Zhang Zhu , Zhao Shuning , Liu Yufeng , Sun Zhouping , Fu Hongdan , Li Tianlai
Published: January 2025

Interactions between endophytes (endophytic bacteria and fungi) and plants are crucial in maintaining crop fitness in agricultural systems, particularly in relation to abundant and rare subcommunities involved in community construction. However, the influence of long-term fertilization on heterogeneous rhizosphere nitrogen and phosphorus environments and how these conditions affect the key subcommunities of root endophytes and their community assembly mechanisms remain unclear. We studied the 26th year of a field experiment conducted in a greenhouse with varying levels of nitrogen and phosphorus (CKP0, CKP1, CNP0, CNP1, ONP0, and ONP1) to assess the composition of tomato root endophytes and their impact on yield. We employed 16S rRNA and fungal ITS region amplicon sequencing to investigate the assembly mechanisms of abundant and rare endophytic subcommunities, network correlations, core subcommunity structures, and key species that enhance crop yield. The results indicated that organic manure and phosphorus fertilizers significantly increased the rhizosphere soil nitrogen content, phosphorus content, and phosphorus availability (labile P, moderately labile P, and non-labile P). These fertilizers also significantly affected the composition (based on Bray-Curtis distance) and community assembly processes (βNTI) of endophytic microbial subcommunities. The assembly of both bacterial and fungal subcommunities was primarily governed by dispersal limitation, with community structures being significantly regulated by the content of rhizosphere soil available nitrogen (AN) and moderately labile P (MLP). Rare bacterial and fungal subcommunities complemented the ecological niches of abundant subcommunities in the co-occurrence network, supporting community functions and enhancing network stability. Nitrogen-adapting abundant and rare bacterial subcommunities provided a stronger predictive correlation for tomato yield than phosphorus-adapting fungal subcommunities. Additionally, three core genera of rare endophytic bacteria such as Arthrobacter, Microbacterium, and Sphingobium were identified as potentially involved in improving crop yield improvement. These findings revealed the distinct assembly mechanisms of endophytic microbial subcommunities affected by fertilization, enhancing our understanding of better management practices and controlling endophytes to improve crop yield in intensive agricultural ecosystems.
The role of Bacillus species in the management of plant-parasitic nematodes
Authors: Vasantha-Srinivasan Prabhakaran , Park Ki Beom , Kim Kil Yong , Jung Woo-Jin , Han Yeon Soo
Published: January 2025

Plant-parasitic nematodes (PPNs), including root-knot nematodes (Meloidogyne spp.), cyst nematodes (Heterodera and Globodera spp.), and other economically significant nematode species, pose severe threats to global agriculture. These nematodes employ diverse survival strategies, such as dormancy in cysts or robust infective juvenile stages. Consequently, their management is challenging. Traditional control methods, such as the use of chemical nematicides, are increasingly scrutinized because of environmental and health concerns. This review focuses on the specific mechanisms employed by Bacillus spp., including nematicidal compound production, systemic resistance induction, and cuticle degradation, to target root-knot and cyst nematodes. These mechanisms offer sustainable solutions for managing nematodes and promoting soil health by enhancing microbial diversity and nutrient cycling. An integrated approach leveraging Bacillus-based biocontrol is proposed to maximize efficacy and agricultural sustainability.
Impact of ovotransferrin on the membrane integrity of Salmonella Enteritidis under egg-white conditions
Authors: Legros Julie , Bonnassie Sylvie , Cochet Marie-Françoise , Jan Sophie , Andrews Simon C. , Baron Florence
Published: January 2025

Introduction

Eggs can mediate foodborne disease resulting in salmonellosis outbreaks that are most commonly caused by Salmonella enterica serovar Enteritidis. Ovotransferrin is a prominent egg-white antimicrobial glycoprotein belonging to the transferrin family, members of which exhibit powerful iron-chelating activity. However, several studies have also described the ability of transferrin proteins to disrupt bacterial membranes. This study aimed to investigate the antimicrobial activity of ovotransferrin toward S. Enteritidis membranes at 30°C under egg-white conditions.

Materials and methods

This aim was supported by the deployment of a synthetic medium designed to mimic egg-white (matching the ionic composition and pH). The ability of ovotransferrin to induce bacterial membrane permeabilization in S. Enteritidis was investigated by measuring substrate accessibility to periplasmic β-lactamase and cytosolic β-galactosidase.

Results

The depolarization of the inner membrane of S. Enteritidis was measured using a fluorescence probe [DiSC3(5)]. The results show that ovotransferrin induces permeabilization of the outer membrane but not the inner membrane whereas egg white permeabilizes both membranes. In addition, the dissipation of the proton motive force by egg white was found to involve a contribution by ovotransferrin since this protein provoked inner-membrane depolarization.

Discussion

It can thus be concluded that ovotransferrin exerts a membranes perturbation activity on S. Enteritidis under egg-white conditions, in addition to its well-known iron-chelation activity.
Alzheimer’s disease diagnosis using rhythmic power changes and phase differences: a low-density EEG study
Authors: Wang Juan , Zhao Jiamei , Chen Xiaoling , Yin Bowen , Li Xiaoli , Xie Ping
Published: January 2025

Objectives

The future emergence of disease-modifying treatments for dementia highlights the urgent need to identify reliable and easily accessible tools for diagnosing Alzheimer’s disease (AD). Electroencephalography (EEG) is a non-invasive and cost-effective technique commonly used in the study of neurodegenerative disorders. However, the specific alterations in EEG biomarkers associated with AD remain unclear when using a limited number of electrodes.

Methods

We studied pathological characteristics of AD using low-density EEG data collected from 26 AD and 29 healthy controls (HC) during both eye closed (EC) and eye opened (EO) resting conditions. The analysis including power spectrum, phase lock value (PLV), and weighted lag phase index (wPLI) and power-to-power frequency coupling (theta/beta) analysis were applied to extract features in the delta, theta, alpha, and beta bands.

Results

During the EC condition, the AD group exhibited decreased alpha power compared to HC. Additionally, both analysis of PLV and wPLI in the theta band indicated that the alterations in the AD brain network predominantly involved in the frontal region with the opposite changes. Moreover, the AD group had increased frequency coupling in the frontal and central regions. Surprisingly, no group difference was found in the EO condition. Notably, decreased theta band functional connectivity within the fronto-central lobe and increased frequency coupling in frontal region were found in AD group from EC to EO. More importantly, the combination of EC and EO quantitative EEG features improved the inter-group classification accuracy when using support vector machine (SVM) in older adults with AD. These findings highlight the complementary nature of EC and EO conditions in assessing and differentiating AD cohorts.

Conclusion

Our results underscore the potential of utilizing low-density EEG data from resting-state paradigms, combined with machine learning techniques, to improve the identification and classification of AD.
Characterizing the hard and soft nanoparticle-protein corona with multilayer adsorption
Authors: Vilanova Oriol , Martinez-Serra Alberto , Monopoli Marco P. , Franzese Giancarlo
Published: January 2025

Nanoparticles (NPs) in contact with biological fluid adsorb biomolecules into a corona. This corona comprises proteins that strongly bind to the NP (hard corona) and loosely bound proteins (soft corona) that dynamically exchange with the surrounding solution. While the kinetics of hard corona formation is relatively well understood, thanks to experiments and robust simulation models, the experimental characterization and simulation of the soft corona present a more significant challenge. Here, we review the current state of the art in soft corona characterization and introduce a novel open-source computational model to simulate its dynamic behavior, for which we provide the documentation. We focus on the case of transferrin (Tf) interacting with polystyrene NPs as an illustrative example, demonstrating how this model captures the complexities of the soft corona and offers deeper insights into its structure and behavior. We show that the soft corona is dominated by a glassy evolution that we relate to crowding effects. This work advances our understanding of the soft corona, bridging experimental limitations with improved simulation techniques.
Fast perceptual learning induces location-specific facilitation and suppression at early stages of visual cortical processing
Authors: Wang Yajie , Qu Zhe , Wang You , Sun Mingze , Mao Mengting , Ding Yulong
Published: January 2025

Tens of minutes of training can significantly improve visual discriminability of human adults, and this fast perceptual learning (PL) effect is usually specific to the trained location, with little transfer to untrained locations. Although location specificity is generally considered as a hallmark of visual PL, it remains unclear whether it involves both facilitation of trained locations and suppression of untrained locations. Here we developed a novel experimental design to investigate the cognitive neural mechanism of location specificity of fast PL. Specifically, we manipulated attentional settings and recorded event-related potentials (ERPs) in both the training and tests. To get reliable location-specific PL effects on early ERPs, we adopted a new approach involving analysis of contralateral-minus-ipsilateral P1 (P1c-i). ERP results showed that tens of minutes of training not only increased the late P1c-i (~100–120 ms) evoked by targets at the trained location, but also decreased the early P1c-i (~75–95 ms) evoked by distractors at the untrained location, both of which were location specific. Moreover, comparison between the pretest and posttest revealed that the suppression effect of early P1c-i preserved even when the untrained location became target location, whereas the facilitation effect of late P1c-i appeared only when the trained location remained actively attended. These findings provide the first evidence that fast PL induces both location-specific facilitation and location-specific suppression at early stages of visual cortical processing. We speculate that while the facilitation effect indicates more efficient allocation of voluntary attention to the trained location induced by fast PL, the suppression effect may reflect learning-associated involuntary suppression of visual processing at the untrained location. Several confounding factors with regard to the early ERP effects of PL are discussed, and some important issues worth further investigation are proposed.
Learning to build low-field MRIs for remote northern communities
Authors: Sarty Gordon E. , Vidarsson Logi , Hansen Christopher , Corrigal Keifer , Sutherland Lionel , Jamieson Millie , Hogue Micheal , Kassahun Haile , Greyeyes William , Teixeira David , Goertzen Lawrence , McEvoy Jonathan , Pollard Mark
Published: January 2025

Low-field Magnetic Resonance Imaging (MRI) has the potential to provide autonomous accessible neuroimaging in remote communities, particularly in the Canadian north. Remoteness necessitates that these MRIs be built and maintained within the communities. This approach not only ensures that the MRIs remain operational but will also allow the youth from the communities to pursue technical careers at home. The first step in this vision is to establish that the technical resources needed for building MRIs are available in remote communities and to establish an educational program that will give students the required technical skills. Over the summer of 2024, a team of students working within an Aircraft Maintenance Engineering (AME) program built the hardware for a wrist-sized prototype MRI. The student team included a high school student, AME students, engineering students and a post doctoral fellow. The skills required to maintain aircraft, namely 3D printing, sheet metal work and electrical harness building, were sufficient to build a low-field MRI. The prototype built was a radio frequency (RF) encoding MRI, whose design was optimized for eventual use in space, but the techniques and procedures developed are applicable to other MRI designs. Furthermore the breadth of students from high school to the post doctoral fellow level facilitated an extremely rich learning environment for the students while they focused on the task of designing and building the prototype MRI. Educational programs around building low-field MRIs can be created at all levels.
A critical period for paired-housing-dependent autistic-like behaviors attenuation in a prenatal valproic acid-induced male mouse model of autism
Authors: Yang Jian-Quan , Yin Bao-Qi , Yang Chao-Hua , Jiang Miao-Miao , Li Zhe
Published: January 2025

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairments in social communication and the presence of restrictive and repetitive behaviors. Investigating the etiological process and identifying an appropriate therapeutic target remain as formidable challenges to overcome ASD due to numerous risk factors and complex symptoms associated with the disorder. Recent studies have indicated that early rehabilitative intervention can alleviate the symptoms of individuals with ASD. However, there remain unsolved issues of behavioral intervention such as the appropriate time and types of therapies. In this study, we employed a mouse model prenatally exposed to valproic acid to establish a validated ASD mouse model and We found that paired-housing with control mice for 4 week after weaning palliated sociability deficits, anxiety and repetitive behaviors in this model of ASD-like behaviors, while paired-housing with their ASD littermate did not produce this effects. Furthermore, by evaluating different time window of paired-housing, we found that paired-housing during postnatal day 21 (P21) to P35, but not P21 to P28 or P35 to P49 or P28 to P35, is a critical period for the influence of paired-housing on autistic-like behaviors. Finally, paired-housing with control mice improved the impaired GABA system in this model of ASD. So our study demonstrates the therapeutic potential of environmental intervention during a critical period in the treatment of ASD.
Modeled sustainability impacts of increasing pork consumption among adults in the United States
Authors: Conrad Zach , Repoulis Vincent , Zavela Catherine
Published: January 2025

Introduction

Little is known about the role of pork in sustainable diet patterns, given that it is often aggregated with other animal proteins or not evaluated at all. To address this gap, this study modeled the sustainability impacts of replacing different protein foods with pork in a nationally representative sample of adults in the United States (US).

Methods

Data on dietary intake, greenhouse gas emissions (GHGE), cumulative energy demand, water scarcity footprint, land, pesticides, fertilizer nutrients, food prices, and diet quality were obtained from publicly available repositories. A food substitution model was constructed to evaluate the change in each sustainability impact when 1–3 servings of beef, poultry, seafood, eggs, or legumes were replaced by pork.

Results

Modeled substitution of beef with pork was associated with reductions in GHGE, land, pesticides, and fertilizer nutrients by 11–35%, and substitution of seafood with pork was associated with reductions in cumulative energy demand by 6% and diet cost by <1%. All other substitutions led to an increase in sustainability impacts of up to 5%, including all outcomes associated with substituting poultry, eggs, and legumes with pork.

Discussion

The US federal government can play an important role in improving data collection methods that distinguish between pork and other meats. This can facilitate further research to evaluate sustainability trade-offs, which can inform clinical practice and public policy to support informed food choices for consumers.
A perspective on intermittent fasting and cardiovascular risk in the era of obesity pharmacotherapy
Authors: Eliopoulos Aristides G. , Gkouskou Kalliopi K. , Tsioufis Konstantinos , Sanoudou Despina
Published: January 2025

Intermittent fasting has been linked to metabolic health by improving lipid profiles, reducing body weight, and increasing insulin sensitivity. However, several randomized clinical trials have shown that intermittent fasting is not more effective than standard daily caloric restriction for short-term weight loss or cardiometabolic improvements in patients with obesity. Observational studies also suggest cardiovascular benefits from extended rather than reduced eating windows, and indicate that long-term intermittent fasting regimens may increase the risk of cardiovascular disease mortality. In this perspective, we discuss evidence that may support potential adverse effects of intermittent fasting on cardiovascular health through the loss of lean mass, circadian misalignment and poor dietary choices associated with reward-based eating. Given the ongoing revolution in obesity pharmacotherapy, we argue that future research should integrate anti-obesity medications with dietary strategies that confer robust benefits to cardiometabolic health, combine exercise regimens, and consider genetic factors to personalize obesity treatment. Comprehensive approaches combining diet, pharmacotherapy, and lifestyle modifications will become crucial for managing obesity and minimizing long-term cardiovascular risk.
Patient engagement in radiation oncology: a large retrospective study of survey response dynamics
Authors: Loving Bailey A. , Ye Hong , Rutka Elizabeth , Robertson John M.
Published: January 2025

Purpose

Patient satisfaction surveys are pivotal in evaluating healthcare quality and enhancing patient care. Understanding the factors influencing patient engagement with these surveys in radiation oncology can guide improvements in patient-centered care.

Methods

This retrospective study analyzed data from radiation oncology patients at a large multi-site single-institution center from May 2021 to January 2024. We assessed the influence of demographic, clinical, and socioeconomic factors on the likelihood of survey participation using univariate (UVA) and multivariable (MVA) logistic regression analyses. Factors included age, gender, race, socioeconomic status (SES) via Area Deprivation Index (ADI), language, marital status, smoking, employment, insurance type, mental health disorders (MHD), comorbidity index (CCI), and cancer type.

Results

In a comprehensive analysis of 11,859 patients, most were female (57.2%), over 65 years old (60.7%), and primarily insured by Medicare (45.9%). MVA showed that higher socioeconomic disadvantage significantly decreased survey participation (ADI third tertile vs. first tertile OR=0.708, p<0.001), with each unit increase in ADI reducing the odds of completion by 1% (p<0.001). Older adults, and patients with head and neck or genitourinary cancers were significantly more likely to participate, while those with higher comorbidities, MHD, or other minority status were less engaged (p<0.001). Telemedicine encounters also significantly increased participation compared to in-person visits (OR=1.149, p=0.006).

Conclusions

Multiple factors including age, race, SES, insurance type, cancer type, health conditions, and modality of healthcare delivery influence patient engagement with satisfaction surveys in radiation oncology. Strategies to enhance patient engagement must consider these diverse influences to ensure comprehensive and inclusive feedback mechanisms in healthcare settings. Tailored interventions to mitigate barriers specific to underrepresented groups are crucial for capturing a broad spectrum of patient experiences and improving the overall quality of patient care.
Major and minor perineural invasion in salivary gland cancer
Authors: Liu Fei , Chu Yinglin , Zheng Qizhe , Hu Yunshuang , Wang Yiyi , Qin Lu , Fu Shuaikun , Wang Suping
Published: January 2025

Objective

To delineate the distribution of perineural invasion (PNI), evaluate its impact on patient survival, and identify optimal criteria for initiating adjuvant radiation therapy (RT) in cases of PNI associated with salivary gland cancer (SGC).

Methods

This retrospective study categorized enrolled patients into three groups based on PNI status (none, minor, or major), defined by the extent of nerve involvement. The influence of PNI on overall survival and locoregional control was assessed using a Cox proportional hazards model.

Results

A total of 555 patients were incorporated into the study. Logistic regression analysis indicated that tumor stage, neck stage, histological grade, and pathological type were independently linked to the occurrence of PNI. In the Cox model assessing overall survival, patients exhibiting minor nerve PNI demonstrated a hazard ratio (HR) of 1.78 [95% CI: 1.14-2.47] in comparison to those without PNI, a difference that was statistically significant (p<0.001). Conversely, the variation in HR between patients with major nerve PNI and those with minor nerve PNI was not statistically significant (p=0.673). In the Cox model for locoregional control, patients with minor and major nerve PNI exhibited HRs of 1.64 [95% CI: 1.17-2.78] and 1.65 [95% CI: 1.03-2.90], respectively, when compared to those without PNI. Subgroup analyses revealed that the incorporation of chemotherapy into radiotherapy did not significantly modify the risk of mortality or locoregional recurrence in comparison to patients treated with radiotherapy alone, irrespective of PNI classification.

Conclusion

Both minor and major nerve PNI exerting comparable influences on prognosis, the adjunctive use of chemotherapy in combination with RT did not yield improvements in overall survival or locoregional control, irrespective of PNI status.
Transarterial radioembolization versus chemoembolization for hepatocellular carcinoma: a meta-analysis
Authors: Lu Wenxiao , Zhang Tongsheng , Xia Fengfei , Huang Xiangzhong , Gao Fulei
Published: January 2025

Background

Currently, inoperable hepatocellular carcinoma (HCC) is treated by both transarterial radioembolization (TARE) and transarterial chemoembolization (TACE). However, their relative efficacy and outcomes remain unclear. This meta-analysis aimed to compare TARE and TACE to evaluate their safety and efficacy in treating inoperable HCC patients.

Methods

Relevant studies were identified by searching the Web of Science, PubMed, and Wanfang databases. Pooled analyses were used to compare treatment response rates, complications, and overall survival (OS) outcomes between the TARE and TACE groups.

Results

This analysis selected 8 studies comprising 1026 and 358 patients that respectively underwent TACE and TARE treatment. The results revealed that the TARE group had significantly higher pooled total response, disease control, and 1-year OS rates compared to the TACE group (P = 0.04, 0.003, and 0.02, respectively), with a corresponding increase in OS (P = 0.0002). Furthermore, rates of complications including fever and abdominal pain were also reduced in the TARE group (P = 0.006 and 0.02, respectively). Moreover, there were no significant differences in the pooled analyses of complete response rates, fatigue, nausea/vomiting, 3-year OS, or 5-year OS between these groups (P = 0.24, 0.69, 0.15, 0.73, and 0.38, respectively). Significant heterogeneity was detected for endpoints including fatigue, nausea/vomiting, fever, abdominal pain, OS duration, and 3-year OS (I2 = 89%, 82%, 72%, 90%, 96%, and 66%, respectively). All endpoints exhibited no significant risk of publication bias.

Conclusions

This study revealed that relative to TACE, TARE performed using 90Y can yield significantly higher treatment response rates and prolong HCC patient survival with fewer treatment-related side effects.

The PRISMA guidelines were used to guide the execution and publication of this meta-analysis. The study is registered at INPLASY.COM (No. INPLASY202380017).

Systematic review registration

INPLASY.COM, identifier INPLASY202380017.
Needle tracking and segmentation in breast ultrasound imaging based on spatio-temporal memory network
Authors: Zhang Qiyun , Chen Jiawei , Wang Jinhong , Wang Haolin , He Yi , Li Bin , Zhuang Zhemin , Zeng Huancheng
Published: January 2025

Introduction

Ultrasound-guided needle biopsy is a commonly employed technique in modern medicine for obtaining tissue samples, such as those from breast tumors, for pathological analysis. However, it is limited by the low signal-to-noise ratio and the complex background of breast ultrasound imaging. In order to assist physicians in accurately performing needle biopsies on pathological tissues, minimize complications, and avoid damage to surrounding tissues, computer-aided needle segmentation and tracking has garnered increasing attention, with notable progress made in recent years. Nevertheless, challenges remain, including poor ultrasound image quality, high computational resource requirements, and various needle shape.

Methods

This study introduces a novel Spatio-Temporal Memory Network designed for ultrasound-guided breast tumor biopsy. The proposed network integrates a hybrid encoder that employs CNN-Transformer architectures, along with an optical flow estimation method. From the Ultrasound Imaging Department at the First Affiliated Hospital of Shantou University, we developed a real-time segmentation dataset specifically designed for ultrasound-guided needle puncture procedures in breast tumors, which includes ultrasound biopsy video data collected from 11 patients.

Results

Experimental results demonstrate that this model significantly outperforms existing methods in improving the positioning accuracy of needle and enhancing the tracking stability. Specifically, the performance metrics of the proposed model is as follows: IoU is 0.731, Dice is 0.817, Precision is 0.863, Recall is 0.803, and F1 score is 0.832. By advancing the precision of needle localization, this model contributes to enhanced reliability in ultrasound-guided breast tumor biopsy, ultimately supporting safer and more effective clinical outcomes.

Discussion

The model proposed in this paper demonstrates robust performance in the computer-aided tracking and segmentation of biopsy needles in ultrasound imaging, specifically for ultrasound-guided breast tumor biopsy, offering dependable technical support for clinical procedures.
Malignant glomus tumor of jejunum with liver and peritoneum metastasis: a rare case report
Authors: Peng Shilan , Yang Nan , Xu Zifan , Su Guomiao , Tuo Xiaoyu , Liu Shiyue , Lei Zi , Pan Guoqing
Published: January 2025

Glomus tumors (GTs) are rare mesenchymal neoplasms that occur predominantly on the subungual region of the distal extremities and are rarely seen in visceral organs such as the gastrointestinal tract. Malignant glomus tumors (MGTs) is even more rare, comprising less than 1% of all GTs. We reported an 82-year-old female patient with MGTs of the jejunum, accompanied by metastases to the liver and peritoneum. The patient presented with a primary complaint of epigastric pain with nausea and acid reflux for two months. Computed tomography scan revealed a prominently enhanced, inhomogeneous-density mass in the jejunum, the nature of which—benign or malignant—could not initially be determined. Postoperative pathological diagnosis confirmed the lesion to be a jejunal MGT. Regrettably, the patient declined additional treatment, subsequently developing liver and peritoneal metastases one year later. She eventually died within 18 months of initial diagnosis. This report summarizes the clinical and histopathological features of jejunal MGTs with the aim of increasing awareness among clinicians and pathologists regarding this disease.
Non-melanoma skin cancer treated with hypofractionated 192—Ir contact brachitherapy: a single institution series
Authors: Ciurlia Elisa , Santo Bianca , Barba Maria Cristina , Cavalera Elisa , De Franco Paola , De Matteis Sara , Di Paola Giuseppe , Leone Angela , Papaleo Antonella , Rubini Dino , Russo Donatella , Rubini Giuseppe , Sardaro Angela
Published: January 2025

Background

Non-melanoma skin carcinoma (NMSC) is the most common malignant tumor in the population, with a steadily increasing incidence due to an aging population and sun exposure. The two main subtypes of NMSC are basal cell carcinoma(BCC) and squamous cell carcinoma(SCC). Therapeutic management of NMSC includes a variety of options, such as surgery, radiotherapy, and topical or systemic treatments. High-dose fractionated contact brachytherapy (c-HDR-BRT) is a viable therapeutic option for treating NMSCs.

Methods

At our center, we treated 39 patients with BCC or SCC, with a total of 46 lesions, treated with c-HDR-BRT. The patients underwent two different radiotherapy schedules: 40 Gy in four fractions and 30 Gy in three fractions.

Results

Two-year results showed 100% local control (LC) and 100% disease-specific survival (DSS), indicating high efficacy of c-HDR-BRT in terms of tumor control. Furthermore, the observed toxicity profile was favorable with no significant late toxicity.

Conclusions

These results suggest that c-HDR-BRT represents a viable therapeutic alternative for NMSC, combining high oncological efficacy with an acceptable safety profile, while minimizing the aesthetic and functional impact of therapy. Finally, the study emphasizes the importance of personalization of treatment and careful evaluation of individual cases to optimize the treatment approach in NMSC.
Evaluating ChatGPT-4o as a decision support tool in multidisciplinary sarcoma tumor boards: heterogeneous performance across various specialties
Authors: Ammo Tekoshin , Guillaume Vincent G. J. , Hofmann Ulf Krister , Ulmer Norma M. , Buenting Nina , Laenger Florian , Beier Justus P. , Leypold Tim
Published: January 2025

Background and objectives

Since the launch of ChatGPT in 2023, large language models have attracted substantial interest to be deployed in the health care sector. This study evaluates the performance of ChatGPT-4o as a support tool for decision-making in multidisciplinary sarcoma tumor boards.

Methods

We created five sarcoma patient cases mimicking real-world scenarios and prompted ChatGPT-4o to issue tumor board decisions. These recommendations were independently assessed by a multidisciplinary panel, consisting of an orthopedic surgeon, plastic surgeon, radiation oncologist, radiologist, and pathologist. Assessments were graded on a Likert scale from 1 (completely disagree) to 5 (completely agree) across five categories: understanding, therapy/diagnostic recommendation, aftercare recommendation, summarization, and support tool effectiveness.

Results

The mean score for ChatGPT-4o performance was 3.76, indicating moderate effectiveness. Surgical specialties received the highest score, with a mean score of 4.48, while diagnostic specialties (radiology/pathology) performed considerably better than the radiation oncology specialty, which performed poorly.

Conclusions

This study provides initial insights into the use of prompt-engineered large language models as decision support tools in sarcoma tumor boards. ChatGPT-4o recommendations regarding surgical specialties performed best while ChatGPT-4o struggled to give valuable advice in the other tested specialties. Clinicians should understand both the advantages and limitations of this technology for effective integration into clinical practice.
Pharmacodynamic characteristics and influencing factors of tapentadol for chronic pain relief under dose titration
Authors: Xin Liang , Zhu Haoxiang , Niu Suping , Han Xie , Pang Hongxian , Li Jiangfan , Hu Ye , Wang Xuhong , Li Lujin , Fang Yi
Published: January 2025

Objective

The aim of this study was to establish a pharmacodynamic model of tapentadol analgesia under dose titration conditions, to quantitatively analyze the time-effect relationship of the drug, and to identify relevant influencing factors. This model is intended to provide a pharmacodynamic reference for designing rational tapentadol dose titration schemes in clinical research.

Methods

Randomized controlled trials assessing the efficacy of tapentadol in the management of chronic pain were retrieved from public databases (PubMed and EMBASE). A time-effect relationship model of the percent change in Numerical Rating Scale (NRS) scores post-tapentadol intervention from baseline was constructed, along with a covariate model to identify factors significantly impacting the analgesic effects of tapentadol. Potential influencing factors that were clinically significant but not included in the final covariate model were examined for their impact trends on tapentadol analgesia through subgroup analysis.

Results

A total of 16 studies involving 4,508 participants were included in the analysis. Covariate analysis indicated that age significantly affected the maximum reduction in NRS scores following tapentadol treatment, with the reduction rate being 40.9% for patients aged 45 and 60.7% for those aged 65, suggesting that older patients have a higher demand for pain relief. Furthermore, studies published after 2014 and placebo-controlled trials showed a slower rate of NRS reduction, indicating a more cautious approach to tapentadol dosing titration post the U.S. opioid crisis and in placebo-controlled contexts. Additionally, subgroup analysis suggested that higher titration doses, higher baseline NRS levels, the use of extended-release tapentadol, and a smaller proportion of male participants were trends associated with better analgesic effects, although the differences were not statistically significant. Moreover, the study found that tapentadol was significantly more effective in treating lower back pain compared to non-lower back pain.

Conclusion

This research successfully developed a pharmacodynamic model for dose-titrated tapentadol administration, which can simulate the temporal changes in analgesic effects of tapentadol across different clinical scenarios. This model can guide the formulation of dosing titration protocols for tapentadol in clinical research.

Systematic Review Registration

https://inplasy.com/inplasy-2024-5-0014/