bluuebunny/crossref_metadata_2025 huggingface dataset:
ABO Incompatible Liver Transplantation in Children: A 20 Year Experience from Centres in the TransplantChild European Reference Network
Authors: Markiewicz-Kijewska Małgorzata , Kaliciński Piotr , Torres Canizales Juan , Di Giorgio Angelo , Baumann Ulrich , Jorns Carl , Baker Alastair , Lopes Maria Francelina , Frauca Remacha Esteban , Lopez-Granados Eduardo , Jara Vega Paloma , Basso Maria-Sole , Kowalewski Grzegorz , Kamińska Diana , Ferreira Sandra , Liccardo Daniela , Pietrobattista Andrea , Spada Marco ,   on behalf of ERN TransplantChild Healthcare Working Group
Published: August 2021

An increasing number of AB0-incompatible (AB0i) liver transplantations (LT) are being undertaken internationally in recent years due to organ shortages and the need for urgent transplantation. The aim of our study was establish the value of ABOi LT from available retrospective results of AB0i pediatric liver transplantations performed in European reference centers now belonging to the TransplantChild, European Reference Network (ERN). Data from medical records were analyzed, including demographic data, diagnosis, urgency of transplantation, time on the waiting list, PELD/MELD score, desensitization procedures, immunosuppression, selected post-transplant complications, and patient and graft survival. A total of 142 patients (pts) with transplants between 1986 and 2018 in 8 European transplant centers were included in the study. The indications for liver transplantation were: cholestatic diseases in 62 pts, acute liver failure in 42 pts, and other conditions in the remaining 38 pts. Sixty-six patients received grafts from living donors, and seventy-six received grafts from deceased donors. Both patient and graft survival were significantly affected by deceased donor type, urgent transplantation, and the development of vascular complications. In the multivariate analysis, vascular complications had a negative impact on patient and graft survival, while a longer time from the first AB0i LT in the study showed better results, suggesting an international learning experience. In conclusion, we believe that AB0i LT in children is now a safe procedure that may be adopted more readily in children.
Parents’ Experience in Children’s Friendship Training Programme for Their Children with Autism Spectrum Disorder: A Qualitative Inquiry
Authors: Ong Sing Yee , Roslan Samsilah , Ahmad Nor Aniza , Ayub Ahmad Fauzi Mohd , Chen Lee Ping , Taresh Sahar Mohammed
Published: August 2021

Background: Children’s Friendship Training (CFT) is a parent-assisted intervention programme that introduces children to basic sets of social rules to help them understand social contexts with specific guidance from their parents. It has been reported in several empirical studies that the friendship skills of children with autism spectrum disorder were enhanced after participating in CFT. However, previous studies only focused on the effectiveness of the training without exploring it from the parent’s perspective. As such, the objective of this study is to highlight the parents’ experience in assisting in the implementation of CFT. Purpose: To explore the parents’ experiences with autism spectrum disorder (ASD) in CFT and examine the experiences using the CFT as a theoretical framework. Methodology: In this study, eight parents and their school-aged children with ASD participated in 12 CFT sessions. Upon completing the CFT, the parents participated in a focus group interview. The interview session was video recorded and transcribed with the parents’ consent. Thematic analysis was employed in analysing the collected data as outlined in six different phases. Results: The generated data revealed the similarities and differences in parents’ experiences in the CFT. The current study has identified four main themes: (1) fear and resistance; (2) awareness, learning, and adjustment; (3) change is hard; and (4) identifying support. Conclusions: The findings highlighted the processes that these parents experienced and encountered while attending the CFT programme, it is important to consider these processes based on how they might impact the effectiveness of the programme. The programme’s effectiveness is reliant on the ability to work closely with parents to understand their challenges and explore the type of support they need. This study has analysed the crucial factors that provide an overview of parents’ encounters in their participation in CFT.
Low-Temperature Plasma Nitriding of 3Cr13 Steel Accelerated by Rare-Earth Block
Authors: You Yuan , Li Rui , Yan Mufu , Yan Jihong , Chen Hongtao , Wang Chaohui , Liu Dongjing , Hong Lin , Han Tingjie
Published: August 2021

The plasma nitriding of 3Cr13 steel occurred at 450 °C for 4, 8 and 12 h in NH3 with and without rare earth (RE). The nitrided layers were characterized using an OM, SEM, TEM, XRD, XPS, microhardness tester and electrochemical workstation. The modified layer, with and without La, are composed of a compound layer and diffusion layer from surface to core. After the addition of La during nitriding, the maximum increase of layer thickness, mass gain and average microhardness was 15.6%, 35.8% and 212.50HV0.05, respectively. With the increase of the proportion of ε-Fe2-3N, the passivation zone of the corrosion resistance curve increases from 2.436 to 3.969 V, the corrosion current density decreases, the corrosion potential and pitting potential both increase, and, consequently, the corrosion resistance is significantly improved. Most of the surface microstructures of the nitrided layer was refined by the addition of La. The presence of La reduces the N content in the modified layer, which accelerates the diffusion of N atoms and, thus, accelerates the nitriding process.
New Records of Cubozoan and Scyphozoan Jellyfish from Sabah Waters, Malaysia
Authors: Chuan Chee Hoe , Venmathi Maran Balu Alagar , Yap Tzuen Kiat , Cheong Kai Ching , Syed Hussein Muhammad Ali , Saleh Ejria
Published: August 2021

Jellyfish play a vital role in the ocean’s ecosystem, acting as a nursery for young fishes, crabs, and brittle stars, as well as a source of food for certain charismatic megafauna, such as sunfish and sea turtles. They also pose a threat to human activities, with jellyfish blooms negatively impacting fisheries, power generation, and tourism. However, very little information is available on the biodiversity of jellyfish within the waters of Borneo. Here, we present new records of jellyfish found along the coast of Sabah, Malaysia, located in northern Borneo, bordering the megadiverse region of the Coral Triangle. A total of six species belonging to two classes and six families hitherto not recorded to the state are reported, Chironex yamaguchii, Acromitus maculosus, Crambione mastigophora, Linuche aquila, Netrostoma sp., and Phyllorhiza punctata. Of these, two species (C. yamaguchii and L. aquila) are harmful to humans, with C. yamaguchii capable of causing human fatalities. Reports of harmful jellyfish are useful to the medical and tourism industry, as some of these species may inflict stings and adverse reactions to humans ranging from rashes and skin irritation to fatal envenomation. A checklist has also been provided for all collected jellyfish species from Sabah waters of Borneo, Malaysia.
Editorial Introduction to the Special Issue “Online and Distance Learning during Lockdown Times: COVID-19 Stories–Series 1”
Authors: Edirisingha Palitha
Published: August 2021

The rapid spread of COVID-19 in early 2020 has changed many aspects of life including that of education all over the world [...]
High Performance Classification Model to Identify Ransomware Payments for Heterogeneous Bitcoin Networks
Authors: Al-Haija Qasem Abu , Alsulami Abdulaziz A.
Published: August 2021

The Bitcoin cryptocurrency is a worldwide prevalent virtualized digital currency conceptualized in 2008 as a distributed transactions system. Bitcoin transactions make use of peer-to-peer network nodes without a third-party intermediary, and the transactions can be verified by the node. Although Bitcoin networks have exhibited high efficiency in the financial transaction systems, their payment transactions are vulnerable to several ransomware attacks. For that reason, investigators have been working on developing ransomware payment identification techniques for bitcoin transactions’ networks to prevent such harmful cyberattacks. In this paper, we propose a high performance Bitcoin transaction predictive system that investigates the Bitcoin payment transactions to learn data patterns that can recognize and classify ransomware payments for heterogeneous bitcoin networks. Specifically, our system makes use of two supervised machine learning methods to learn the distinguishing patterns in Bitcoin payment transactions, namely, shallow neural networks (SNN) and optimizable decision trees (ODT). To validate the effectiveness of our solution approach, we evaluate our machine learning based predictive models on a recent Bitcoin transactions dataset in terms of classification accuracy as a key performance indicator and other key evaluation metrics such as the confusion matrix, positive predictive value, true positive rate, and the corresponding prediction errors. As a result, our superlative experimental result was registered to the model-based decision trees scoring 99.9% and 99.4% classification detection (two-class classifier) and accuracy (multiclass classifier), respectively. Hence, the obtained model accuracy results are superior as they surpassed many state-of-the-art models developed to identify ransomware payments in bitcoin transactions.
Soil Erosion Prediction Based on Moth-Flame Optimizer-Evolved Kernel Extreme Learning Machine
Authors: Chen Chengcheng , Wang Xianchang , Wu Chengwen , Mafarja Majdi , Turabieh Hamza , Chen Huiling
Published: August 2021

Soil erosion control is a complex, integrated management process, constructed based on unified planning by adjusting the land use structure, reasonably configuring engineering, plant, and farming measures to form a complete erosion control system, while meeting the laws of soil erosion, economic and social development, and ecological and environmental security. The accurate prediction and quantitative forecasting of soil erosion is a critical reference indicator for comprehensive erosion control. This paper applies a new swarm intelligence optimization algorithm to the soil erosion classification and prediction problem, based on an enhanced moth-flame optimizer with sine–cosine mechanisms (SMFO). It is used to improve the exploration and detection capability by using the positive cosine strategy, meanwhile, to optimize the penalty parameter and the kernel parameter of the kernel extreme learning machine (KELM) for the rainfall-induced soil erosion classification prediction problem, to obtain more-accurate soil erosion classifications and the prediction results. In this paper, a dataset of the Vietnam Son La province was used for the model evaluation and testing, and the experimental results show that this SMFO-KELM method can accurately predict the results, with significant advantages in terms of classification accuracy (ACC), Mathews correlation coefficient (MCC), sensitivity (sensitivity), and specificity (specificity). Compared with other optimizer models, the adopted method is more suitable for the accurate classification of soil erosion, and can provide new solutions for natural soil supply capacity analysis, integrated erosion management, and environmental sustainability judgment.
Designing 1D Chaotic Maps for Fast Chaotic Image Encryption
Authors: Khairullah Mustafa Kamil , Alkahtani Ammar Ahmed , Bin Baharuddin Mohd Zafri , Al-Jubari Ammar Mohammed
Published: August 2021

Chaotic maps that can provide highly secure key sequences and ease of structure implementation are predominant requirements in image encryption systems. One Dimensional (1D) chaotic maps have the advantage of a simple structure and can be easily implemented by software and hardware. However, key sequences produced by 1D chaotic maps are not adequately secure. Therefore, to improve the 1D chaotic maps sequence security, we propose two chaotic maps: 1D Improved Logistic Map (1D-ILM) and 1D Improved Quadratic Map (1D-IQM). The proposed maps have shown higher efficiency than existing maps in terms of Lyapunov exponent, complexity, wider chaotic range, and higher sensitivity. Additionally, we present an efficient and fast encryption method based on 1D-ILM and 1D-IQM to enhance image encryption system performance. This paper also introduces a key expansion method to reduce the number of chaotic map iteration needs, thereby decreasing encryption time. The security analyses and experimental results are confirmed that 2D Correlation Coefficient (CC) Information Entropy (IE), Number of Pixels Change Rate (NPCR), Unified Average Changing Intensity (UACI), Mean Absolute Error (MAE), and decryption quality are able to meet the encryption security demands (CC = −0.00139, IE = 7.9990, NPCR = 99.6114%, UACI = 33.46952% and MAE = 85.3473). Furthermore, the proposed keyspace reaches 10240, and the encryption time is 0.025s for an image with a size of 256 × 256. The proposed system can yield efficacious security results compared to obtained results from other encryption systems.
Review on LPV Approaches for Suspension Systems
Authors: Sename Olivier
Published: August 2021

This paper presents a detailed literature review about Linear Parameter Varying (LPV) approaches applied to vehicle suspension systems. Indeed many works have been devoted to vehicle (active and semi-active) suspension in the past 20 years, because this subsystem in the only one affecting passenger comfort and road holding. Moreover several studies have also been concerned with global vehicle dynamic control using the suspension systems in collaboration with other subsystems (steering, braking …). On the other hand, the LPV approaches have proved to be very efficient to control non linear systems as well as to provide some kind of adaptive control. Naturally many LPV methods have been developed for suspension systems in order to take into account the nonlinear characteristics of the dampers, to adapt the suspension performance to the passenger request or to the road profile, to make the suspension systems collaborate with other subsystems, or to provide a fault tolerant control in case of damper loss of efficiency. This survey paper will make a deep analysis about the recent studies dedicated to vehicle suspension systems aiming at providing a better insight on the type of LPV methods that have been considered.
A Simulation Study on Risks to Wind Turbine Arrays from Thunderstorm Downbursts in Different Atmospheric Stability Conditions
Authors: Lu Nan-You , Manuel Lance , Hawbecker Patrick , Basu Sukanta
Published: August 2021

Thunderstorm downbursts have been reported to cause damage or failure to wind turbine arrays. We extend a large-eddy simulation model used in previous work to generate downburst-related inflow fields with a view toward defining correlated wind fields that all turbines in an array would experience together during a downburst. We are also interested in establishing what role contrasting atmospheric stability conditions can play on the structural demands on the turbines. This interest is because the evening transition period, when thunderstorms are most common, is also when there is generally acknowledged time-varying stability in the atmospheric boundary layer. Our results reveal that the structure of a downburst’s ring vortices and dissipation of its outflow play important roles in the separate inflow fields for turbines located at different parts of the array; these effects vary with stability. Interacting with the ambient winds, the outflow of a downburst is found to have greater impacts in an “average” sense on structural loads for turbines farther from the touchdown center in the stable cases. Worst-case analyses show that the largest extreme loads, although somewhat dependent on the specific structural load variable considered, depend on the location of the turbine and on the prevailing atmospheric stability. The results of our calculations show the highest simulated foreaft tower bending moment to be 85.4 MN-m, which occurs at a unit sited in the array farther from touchdown center of the downburst initiated in a stable boundary layer.
A Generic Pipeline for Machine Learning Users in Energy and Buildings Domain
Authors: Abbass Mahmoud Abdelkader Bashery , Hamdy Mohamed
Published: August 2021

One of the biggest problems in applying machine learning (ML) in the energy and buildings field is the lack of experience of ML users in implementing each ML algorithm in real-life applications the right way, because each algorithm has prerequisites to be used and specific problems or applications to be implemented. Hence, this paper introduces a generic pipeline to the ML users in the specified field to guide them to select the best-fitting algorithm based on their particular applications and to help them to implement the selected algorithm correctly to achieve the best performance. The introduced pipeline is built on (1) reviewing the most popular trails to put ML pipelines for the energy and building, with a declaration for each trial drawbacks to avoid it in the proposed pipeline; (2) reviewing the most popular ML algorithms in the energy and buildings field and linking them with possible applications in the energy and buildings field in one layout; (3) a full description of the proposed pipeline by explaining the way of implementing it and its environmental impacts in improving energy management systems for different countries; and (4) implementing the pipeline on real data (CBECS) to prove its applicability.
A Petrostate’s Outlook on Low-Carbon Transitions: The Discursive Frames of Petroleum Policy in Norway
Authors: Handeland Tine S. , Langhelle Oluf
Published: August 2021

Norway is a petroleum exporting country that, simultaneously, is at the forefront of implementing ambitious climate policy measures. Through a discourse analysis of official documents that address petroleum policy, this article examines how the Norwegian government justifies a place for Norwegian petroleum in a low-carbon future. Our findings show that the frames used to justify continued petroleum production between 2011 and 2018 remains predominantly stable, despite the growing opposition to this official discourse in relation to climate change and the societal dependence on petroleum revenues. This article highlights the tension that Norway, as a petroleum-producing country, face in an increasingly carbon-constrained world, and how this is handled in the official petroleum discourse. It shows how the official discourse portrays continued petroleum production and exploration as both valid and necessary and how this framing is discursively linked to a strong commitment to mitigate climate change.
Species-Specific Effects of Groundwater Level Alteration on Climate Sensitivity of Floodplain Trees
Authors: Šenfeldr Martin , Horák Pavel , Kvasnica Jakub , Šrámek Martin , Hornová Hana , Maděra Petr
Published: August 2021

European floodplain forest is facing increasingly frequent and severe drought events related with ongoing climate change. Moreover, this ecosystem type was frequently affected by river regulation, leading to groundwater table lowering; however, river revitalization has, in some locations, achieved some restoration of groundwater levels. In this study, we investigated the growth–climate sensitivity and growth modulation after groundwater-level manipulation for Fraxinus angustifolia Vahl. and Quercus robur L. in one of the most important floodplain forest complexes in Central Europe. We constructed three different types of tree ring chronologies to reflect the high frequency variability, medium-low frequency variability, and basal area increment. We found F. angustifolia to be more sensitive than Q. robur to both drought and groundwater level fluctuations. Moreover, F. angustifolia showed more pronounced short-term and long-term growth decreases after artificial ground water level alteration than did Q. robur. We also found that the groundwater level increase due to river revitalization reduced the climate sensitivity for both F. angustifolia and Q. robur. The decrease in climate sensitivity associated with revitalization was more pronounced for F. angustifolia which, moreover, showed a greater basal growth after river revitalization. Our results suggest that F. angustifolia will be more threatened than Q. robur by the diminution in groundwater availability and increase in drought with ongoing climate change. They also show that river revitalization can be a suitable management tool to help the adaptation to climate change.
Linking Vegetation, Soil Carbon Stocks, and Earthworms in Upland Coniferous–Broadleaf Forests
Authors: Kuznetsova Anastasiia I. , Geraskina Anna P. , Lukina Natalia V. , Smirnov Vadim E. , Tikhonova Elena V. , Shevchenko Nikolay E. , Gornov Aleksey V. , Ruchinskaya Elena V. , Tebenkova Daria N.
Published: August 2021

Linking vegetation, soil biota, and soil carbon stocks in forests has a high predictive value. The specific aim of this study was to identify the relationships between vegetation, earthworms, and soil carbon stocks in nine types of forests dominating autonomous landscape positions in a coniferous–broadleaf forest zone of the European part of Russia. Mountain forests were selected in the Northwest Caucasus, while plain forests were selected in Bryansk Polesie and on the Moskva-Oka plain. One-way analysis of variance (ANOVA) and v-tests were used to assess the impact of different factors on soil C stocks. To assess the contribution of vegetation, litter quality, and earthworms to variation of carbon stocks in organic (FH-layer) and mineral layer (0–50 cm), the method of hierarchical partitioning was performed. The highest C stocks in the organic horizons were associated with the low-quality litter, i.e., with a low base saturation, high acidity, and wide C/N ratio. The highest soil C stocks in the mineral layers were found in mixed forests with the highest richness of plant species, producing litterfall of different quality. The C stock in the organic horizon was negatively related to the biomass of worms that process the litter, while the carbon stock in the mineral layers was positively related to the biomass of worms whose life activity is related to the mineral layers. These findings demonstrated the substantial influence of plants producing a litter of different quality, and of earthworms, belonging to different functional groups, on soil C stocks in coniferous–broadleaf forests.
Phase Evolution of the Time- and Space-Like Peregrine Breather in a Laboratory
Authors: He Yuchen , Suret Pierre , Chabchoub Amin
Published: August 2021

Coherent wave groups are not only characterized by the intrinsic shape of the wave packet, but also by the underlying phase evolution during the propagation. Exact deterministic formulations of hydrodynamic or electromagnetic coherent wave groups can be obtained by solving the nonlinear Schrödinger equation (NLSE). When considering the NLSE, there are two asymptotically equivalent formulations, which can be used to describe the wave dynamics: the time- or space-like NLSE. These differences have been theoretically elaborated upon in the 2016 work of Chabchoub and Grimshaw. In this paper, we address fundamental characteristic differences beyond the shape of wave envelope, which arise in the phase evolution. We use the Peregrine breather as a referenced wave envelope model, whose dynamics is created and tracked in a wave flume using two boundary conditions, namely as defined by the time- and space-like NLSE. It is shown that whichever of the two boundary conditions is used, the corresponding local shape of wave localization is very close and almost identical during the evolution; however, the respective local phase evolution is different. The phase dynamics follows the prediction from the respective NLSE framework adopted in each case.
A Conceptual Framework for Multi-Dimensional Measurements of Food Related Pleasure—The Food Pleasure Scale
Authors: Andersen Barbara Vad , Chan Raymond C. K. , Byrne Derek Victor
Published: August 2021

In modern times, the majority of food intake is believed to be driven by hedonic processes, rather than homeostatic ones. Various factors have been found to influence the hedonic eating experience and thereby influence eating behaviour, and each factor can be regarded a piece that contributes to parts of the total picture of the hedonic response to food. As a result, the literature on the hedonic response to food-related experiences is comprehensive, but at the same time rather fragmented; and importantly, it is not clear how individuals/segments differ in key drivers of their hedonic experience and the extent to which food pleasure is perceived. In this paper, we present a conceptual framework for the development of a scale (self-report questionnaire) to measure the qualitative and quantitative aspects of food-related pleasure, the Food Pleasure Scale. We introduce the concept of (an)hedonia and scales developed in the past for its measurement, identify the spectrum of characteristics influencing food-related pleasure and explain the relevance of developing such a scale. Based on this theoretical framework, a strategy for the development of the Food Pleasure Scale is proposed.
Effects of Herring Milt Hydrolysates and Fractions in a Diet-Induced Obesity Model
Authors: Benoit Noémie , Dubois Marie-Julie , Pilon Geneviève , Varin Thibault V. , Marette André , Bazinet Laurent
Published: August 2021

Over the past years, promising results from studies have shown that herring milt hydrolysates (HMH) can counter immune-metabolic disorders associated with obesity. However, more studies must corroborate these results. Thus, three commercial hydrolysates (HMH1, HMH2, and HMH3) as well as the fractions of two of them (HMH4 and HMH5) obtained by electrodialysis with ultrafiltration membranes (EDUF) were evaluated in vivo at higher doses compared to a previous study. To achieve this, seven groups of mice were fed for 8 weeks with either a control Chow diet or an obesogenic diet rich in fat and sucrose (HFHS) and supplemented by daily gavage with water or 312.5 mg/kg of one of the five HMH products. In summary, HMH supplements had no impact on weight gain. In the insulin tolerance test (ITT), HMH2 and its HMH5 fraction significantly reduced the blood sugar variation (p < 0.05). However, during the glucose tolerance (OGTT), HMH2 supplement increased the hyperinsulinemia variation (p < 0.05) induced by the HFHS diet. HMH1, HMH2, and HMH5 supplements generated potentially beneficial changes for health in the gut microbiota. These results reveal that HMH do not counteract obesity effects but may decrease certain physiological effects induced by obesity.
Effects of Tartary Buckwheat Bran Flour on Dough Properties and Quality of Steamed Bread
Authors: Zhang Sheng , Chen Si , Geng Sheng , Liu Changzhong , Ma Hanjun , Liu Benguo
Published: August 2021

Steamed bread is a traditional staple food of China. Replacing wheat flour (WF) with Tartary buckwheat is expected to improve the nutritional value of steamed bread. In this study, Tartary buckwheat flour (TBF), Tartary buckwheat bran flour (TBBF), and Tartary buckwheat core flour (TBCF) were prepared, their composition and physicochemical properties were compared. It was found that TBBF had higher protein and rutin contents, so its antioxidant activity and dough rheological properties were obviously superior to those of TBF and TBCF. When TBBF was mixed with WF, its weight proportion in the blend (Wbran) had a significant effect on the dough rheological properties. When Wbran was 30%, the dough had the optimal mixing tolerance, and when Wbran exceeded 30%, it caused dilution effect, weakening the gluten network. With the increase of Wbran, the color of the steamed bread developed by the TBBF–WF blend gradually darkened and yellowed, the specific volume declined, and its hardness, gumminess, and chewiness ascended gradually. The appropriate addition of TBBF (Wbran = 10% and 30%) was beneficial to cell diameter and volume of steamed bread, but the further rise of Wbran would destroy its gas retention ability. The predicted glycemic index (pGI) of steamed bread declined significantly with the increasing Wbran.
Environmental Enrichment Rescues Social Behavioral Deficits and Synaptic Abnormalities in Pten Haploinsufficient Mice
Authors: Clipperton-Allen Amy E. , Zhang Angela , Cohen Ori S. , Page Damon Theron
Published: August 2021

Pten germline haploinsufficient (Pten+/−) mice, which model macrocephaly/autism syndrome, show social and repetitive behavior deficits, early brain overgrowth, and cortical–subcortical hyperconnectivity. Previous work indicated that altered neuronal connectivity may be a substrate for behavioral deficits. We hypothesized that exposing Pten+/− mice to environmental enrichment after brain overgrowth has occurred may facilitate adaptation to abnormal “hard-wired” connectivity through enhancing synaptic plasticity. Thus, we reared Pten+/− mice and their wild-type littermates from weaning under either standard (4–5 mice per standard-sized cage, containing only bedding and nestlet) or enriched (9–10 mice per large-sized cage, containing objects for exploration and a running wheel, plus bedding and nestlet) conditions. Adult mice were tested on social and non-social assays in which Pten+/− mice display deficits. Environmental enrichment rescued sex-specific deficits in social behavior in Pten+/− mice and partially rescued increased repetitive behavior in Pten+/− males. We found that Pten+/− mice show increased excitatory and decreased inhibitory pre-synaptic proteins; this phenotype was also rescued by environmental enrichment. Together, our results indicate that environmental enrichment can rescue social behavioral deficits in Pten+/− mice, possibly through normalizing the excitatory synaptic protein abundance.
Geoconservation in the Cabeço da Ladeira Paleontological Site (Serras de Aire e Candeeiros Nature Park, Portugal): Exquisite Preservation of Animals and Their Behavioral Activities in a Middle Jurassic Carbonate Tidal Flat
Authors: Machado Susana , Mergulhão Lia , Pereira Bruno Claro , Pereira Pedro , Carvalho Jorge , Anacleto José António , Neto de Carvalho Carlos , Belo João , Paredes Ricardo , Baucon Andrea
Published: August 2021

The Cabeço da Ladeira paleontological site in central Portugal became known as the “Jurassic Beach”. Formerly an active quarry, the site was protected in order to preserve the large exposures of a Middle Jurassic (early? Bajocian) carbonate tidal flat with an outstanding fossil record. This multidisciplinary paleontological work involving experts from several national and international research and geoconservation institutions was carried out under the umbrella of the Institute for Nature Conservation (ICNF), since this geosite is located within the Serras de Aire e Candeeiros nature park. Cabeço da Ladeira has provided exquisitely preserved body fossils, especially echinoderms, together with the exceptional preservation of their, and other, animal’s behaviors. It is also a hotspot to understand the diversity of bivalves in the Middle Jurassic. Due to the large area of the geosite, the international relevance of the findings, and the risk of weathering and destruction of the fossils, a geoconservation plan has been developed by ICNF with the support of local authorities. After several years of being open to visitors without proper control, the Cabeço da Ladeira paleontological site is now conditioned to organized groups of researchers, schools, and tourists. Some body fossils were collected for studies and included in the national collections of the Geological Museum (Lisbon). Moreover, casts have been made to protect holotypes of trace fossils, also providing ways to replicate this fossil record in temporary exhibitions. A long-term experimental study to conserve the limestone bedding plane exposures and their fossil contents was started in order to develop the best geoconservation strategy with an aim to reduce the damage produced by the increasing tourist demand on natural sites. Cabeço da Ladeira and other geosites in protected areas are key to communicating an evolutionary approach to environmental education, and their geoconservation must be a priority to improve their long-term use as (geo)tourism attractions.
SARS-CoV-2: Origin, Intermediate Host and Allergenicity Features and Hypotheses
Authors: Huang Yuyi , Xie Junmou , Guo Yuhe , Sun Weimin , He Ying , Liu Kequn , Yan Jie , Tao Ailin , Zhong Nanshan
Published: August 2021

The goal of this study is to investigate the probable intermediate hosts and the allergenicity of the notorious virus SARS-CoV-2 to understand how this virus emerged. The phylogenetic analysis of the virus spike proteins indicates that SARS-CoV-2 falls into various small subclades that include a bat coronavirus RaTG13, suggesting bats as a likely natural origin. Refined alignment of the spike protein in NCBI found several fragments that are specific to SARS-CoV-2 and/or SARS-CoV are specific to Rattus norvegicus and/or Mus musculus, suggesting that rodents are the intermediate reservoir of SARS-CoV-2 and SARS-CoV. To evaluate the allergenicity values, the binding affinities of human leukocyte antigen (HLA) class I or II molecules with the spike proteins were calculated, and the results showed that both SARS-CoV-2 and SARS-CoV are predicted to bind to fourteen HLA class I and II molecules with super-high HLA allele-peptide affinities. The infection rate of individuals who have HLA alleles with very high binding affinities who might become infected and develop into refractory patients if there were no medical or non-medical interventions is about 7.36% and 4.78% of Chinese and Americans, respectively. Extremely high temperature and exceptionally low precipitation, the common climate factors between the outbreak sites of COVID-19 in Wuhan in 2019 and SARS in Guangdong in 2002, might have promoted coronavirus evolution into more virulent forms. Our hypothesis suggests that early immunization with an allergenically-engineered virus, in combination with continued surveillance of meteorological factors and viral mutations, may be one of the most powerful prophylactic modalities to fight this virus.
A Longitudinal Study on the Relationship between Physical Education and Social Health
Authors: Cheon Hanguk , Lim Seijun
Published: August 2021

This study aimed to examine whether school physical education (PE) promotes students’ social health using a longitudinal design. To this end, data from 1979 students from a 4th grade cohort, established by the Korean Children and Youth Panel Survey with data collected from 2010 to 2016, who participated in all of the 7th grade (2013), 8th grade (2014), and 9th grade (2015) surveys were analyzed. We used autoregressive cross-lagged structural equation modeling conducted with AMOS 23.0 to examine the longitudinal causality among the factors. The following results were obtained. First, there was reciprocal causation between PE and social health (PE→SR, β = 0.099, β = 0.100; SR→PE; β = 0.207, β = 0.226). Second, PE did not influence sense of community, whereas the latter had a negative effect on PE (β = −0.078, β = −0.077). Third, social relationships influenced the sense of community (β = 0.248, β = 0.266). Based on these findings, we suggest the need for a system that enables students to frequently monitor their performance. This includes implementing a program to improve social relationships to enhance the quality of PE participation, designing programs that foster a sense of community in PE in Korean middle schools, and structuring PE programs that consider the hierarchy between social relationships and a sense of community.
Effect of Distraction Intervention for Needle-Related Pain and Distress in Children: A Systematic Review and Meta-Analysis
Authors: Cho Mi-Kyoung , Choi Mi-Young
Published: August 2021

A systematic review and meta-analysis conducted to evaluate the combined effect of distraction intervention for needle-related pain in order to provide the basis for developing an effective nursing intervention for children. We searched three electronic databases, PubMed, Embase, and CINAHL, for original articles published in the period from 1 January 2011 to 31 July 2019. In addition, a manual search was performed on the basis of references in the literature and the references of the articles in pursuit of comprehensive data until 10 December 2019. Meta-analysis was performed by the synthesis of the effect size, homogeneity, heterogeneity, and trim-and-fill method using MIX 2.0 Pro. Well-planned RCTs, single-center studies, high-quality studies, participants older than 10 years of age, and visual and clown distraction interventions were effective for needle-related pain and distress management among children. The results showed evidence supporting the effect of distraction interventions for children’s needle-related pain and distress. Through this review, strategies were identified to design better interventions to improve the outcomes.
The Effect of Hyperbaric Therapy on Brown Adipose Tissue in Rats
Authors: Lee Chang-Hyung , Choi Young-A , Heo Sung-Jin , Song Parkyong
Published: August 2021

Brown adipose tissue (BAT) plays an important role in thermogenic regulation, which contributes to alleviating diet-induced obesity through uncoupling protein 1 (UCP1) expression. While cold exposure and physical exercise are known to increase BAT development and UCP1 expression, the contribution of hyperbaric oxygen (HBO) therapy to BAT maturation remains largely unknown. Here, we show that HBO treatment sufficiently increases BAT volumes and thermogenic protein levels in Sprague-Dawley rats. Through 18F-FDG PET/CT analysis, we found that exposure to high-pressure oxygen (1.5–2.5 ATA) for 7 consecutive days increased radiolabeled glucose uptake and BAT development to an extent comparable to cold exposure. Consistent with BAT maturation, thermogenic protein levels, such as those of UCP1 and peroxisome proliferator-activated receptor γ coactivator 1α (PGC−1α), were largely increased by HBO treatment. Taken together, we suggest HBO therapy as a novel method of inducing BAT development, considering its therapeutic potential for the treatment of metabolic disorders.
Fatal Clostridium Infection in a Leg-Amputated Patient after Unsuccessful Knee Arthroplasty
Authors: Zardi Enrico Maria , Persichetti Paolo , Palumbo Alessio , Franceschetti Edoardo , Franceschi Francesco
Published: August 2021

Prosthetic joint infection (PJI) is a possible complication occurring after prosthesis implantation. We describe the case of a patient with early postoperative multidrug-resistant polymicrobial PJI and mixed infection of the surgical wound. Despite the removal of the prosthesis, the positioning of double-stage exchange, and dehiscence debridement of the surgical wound, the infection continued. Positioning of an external fixator, plastic reconstruction with a skin graft, and continuous (two years) multiple antimicrobial therapy led to the resolution of the knee infection; a knee prosthesis was implanted, but a new infection of the extensus apparatus by multidrug-resistant Klebsiella pnumoniae followed. It was complicated by surgical wound dehiscence, forcing us to remove the prosthesis, put a new external fixator, and continue with the antibiotic treatment, with no results, and, finally, proceed to a leg amputation. Fourteen days after, the patient was discharged in good clinical condition but, fifteen days later, during rehabilitation in another hospital, the patient developed a severe Clostridium difficilis infection with profuse, intense diarrhea, toxic megacolon, and septic shock; despite colectomy and treatment in an intensive care unit, he died four months later. Patients affected by polymicrobial PJI are at high risk of treatment failure and, therefore, should be given a warning, in good time and appropriate form, of the likelihood of leg amputation.
Role of EphA4 in Mediating Motor Neuron Death in MND
Authors: Zhao Jing , Stevens Claire H. , Boyd Andrew W. , Ooi Lezanne , Bartlett Perry F.
Published: August 2021

Motor neuron disease (MND) comprises a group of fatal neurodegenerative diseases with no effective cure. As progressive motor neuron cell death is one of pathological characteristics of MND, molecules which protect these cells are attractive therapeutic targets. Accumulating evidence indicates that EphA4 activation is involved in MND pathogenesis, and inhibition of EphA4 improves functional outcomes. However, the underlying mechanism of EphA4’s function in MND is unclear. In this review, we first present results to demonstrate that EphA4 signalling acts directly on motor neurons to cause cell death. We then review the three most likely mechanisms underlying this effect.
Peroxiredoxin 1 Controls Ovulation and Ovulated Cumulus–Oocyte Complex Activity through TLR4-Derived ERK1/2 Signaling in Mice
Authors: Park Hyo-Jin , Kim Bokyung , Koo Deog-Bon , Lee Dong-Seok
Published: August 2021

Peroxiredoxins (PRDXs) are expressed in the ovary and during ovulation. PRDX1 activity related to the immuno-like response during ovulation is unknown. We investigated the roles of Prdx1 on TLR4 and ERK1/2 signaling from the ovulated cumulus–oocyte complex (COC) using Prdx1-knockout (K/O) and wild-type (WT) mice. Ovulated COCs were collected 12 and 16 h after pregnant mare serum gonadotropin/hCG injection. PRDX1 protein expression and COC secretion factors (Il-6, Tnfaip6, and Ptgs2) increased 16 h after ovulated COCs of the WT mice were obtained. We treated the ovulated COCs in mice with LPS (0.5 μg/mL) or hyaluronidase (Hya) (10 units/mL) to induce TLR4 activity. Intracellular reactive oxygen species (ROS), cumulus cell apoptosis, PRDX1, TLR4/P38/ERK1/2 protein expression, and COC secretion factors’ mRNA levels increased in LPS- and Hya-treated COCs. The ERK inhibitor (U0126) and Prdx1 siRNA affected TLR4/ERK1/2 expression. The number and cumulus expansion of ovulated COCs by ROS were impaired in Prdx1 K/O mice but not in WT ones. Prdx1 gene deletion induced TLR4/P38/ERK1/2 expression and cumulus expansion genes. These results show the controlling roles of PRDX1 for TLR4/P38/ERK1/2 signaling activity in ovulated mice and the interlink of COCs with ovulation.
The Metabolic Flux Probe (MFP)—Secreted Protein as a Non-Disruptive Information Carrier for 13C-Based Metabolic Flux Analysis
Authors: Dusny Christian , Schmid Andreas
Published: August 2021

Novel cultivation technologies demand the adaptation of existing analytical concepts. Metabolic flux analysis (MFA) requires stable-isotope labeling of biomass-bound protein as the primary information source. Obtaining the required protein in cultivation set-ups where biomass is inaccessible due to low cell densities and cell immobilization is difficult to date. We developed a non-disruptive analytical concept for 13C-based metabolic flux analysis based on secreted protein as an information carrier for isotope mapping in the protein-bound amino acids. This “metabolic flux probe” (MFP) concept was investigated in different cultivation set-ups with a recombinant, protein-secreting yeast strain. The obtained results grant insight into intracellular protein turnover dynamics. Experiments under metabolic but isotopically nonstationary conditions in continuous glucose-limited chemostats at high dilution rates demonstrated faster incorporation of isotope information from labeled glucose into the recombinant reporter protein than in biomass-bound protein. Our results suggest that the reporter protein was polymerized from intracellular amino acid pools with higher turnover rates than biomass-bound protein. The latter aspect might be vital for 13C-flux analyses under isotopically nonstationary conditions for analyzing fast metabolic dynamics.
MicroRNA Let-7e in the Mouse Prefrontal Cortex Differentiates Restraint-Stress-Resilient Genotypes from Susceptible Genotype
Authors: Solich Joanna , Kolasa Magdalena , Faron-Górecka Agata , Hajto Jacek , Piechota Marcin , Dziedzicka-Wasylewska Marta
Published: August 2021

Three strains of mice with various susceptibilities to restraint stress (RS), i.e., mice with a knocked out norepinephrine transporter gene (NET-KO), SWR/J and C57BL/6J (WT) mice were shown to serve as a good model to study the molecular mechanisms underlying different stress-coping strategies. We identified 14 miRNAs that were altered by RS in the PFC of these mice in a genotype-dependent manner, where the most interesting was let-7e. Further in silico analysis of its potential targets allowed us to identify five mRNAs (Bcl2l11, Foxo1, Pik3r1, Gab1 and Map2k4), and their level alterations were experimentally confirmed. A next-generation sequencing (NGS) approach, which was employed to find transcripts differentially expressed in the PFC of NET-KO and WT mice, showed that, among others, two additional mRNAs were regulated by mmu-let-7e, i.e., mRNAs that encode Kmt2d and Inf2. Since an increase in Bcl2l11 and Pik3r1 mRNAs upon RS in the PFC of WT mice resulted from the decrease in mmu-let-7e and mmu-miR-484 regulations, we postulated that MAPK, FoxO and PI3K-Akt signaling pathways were associated with stress resilience, although via different, genotype-dependent regulation of various mRNAs by let-7e and miR-484. However, a higher level of Kmt2d mRNA (regulated by let-7e) that was found with NGS analysis in the PFC of NET-KO mice indicated that histone methylation was also important for stress resilience.
The Second-Generation PIM Kinase Inhibitor TP-3654 Resensitizes ABCG2-Overexpressing Multidrug-Resistant Cancer Cells to Cytotoxic Anticancer Drugs
Authors: Wu Chung-Pu , Li Yan-Qing , Chi Ya-Chen , Huang Yang-Hui , Hung Tai-Ho , Wu Yu-Shan
Published: August 2021

Human ATP-binding cassette (ABC) subfamily G member 2 (ABCG2) mediates the transport of a wide variety of conventional cytotoxic anticancer drugs and molecular targeted agents. Consequently, the overexpression of ABCG2 in cancer cells is linked to the development of the multidrug resistance (MDR) phenotype. TP-3654 is an experimental second-generation inhibitor of PIM kinase that is currently under investigation in clinical trials to treat advanced solid tumors and myelofibrosis. In this study, we discovered that by attenuating the drug transport function of ABCG2, TP-3654 resensitizes ABCG2-overexpressing multidrug-resistant cancer cells to cytotoxic ABCG2 substrate drugs topotecan, SN-38 and mitoxantrone. Moreover, our results indicate that ABCG2 does not mediate resistance to TP-3654 and may not play a major role in the induction of resistance to TP-3654 in cancer patients. Taken together, our findings reveal that TP-3654 is a selective, potent modulator of ABCG2 drug efflux function that may offer an additional combination therapy option for the treatment of multidrug-resistant cancers.