bluuebunny/crossref_metadata_2025 huggingface dataset:
Protection by Ankle Brace for Lower-Extremity Joints in Half-Squat Parachuting Landing With a Backpack
Authors: Jiang Tianyun , Tian Shan , Chen Tianhong , Fan Xingyu , Yao Jie , Wang Lizhen
Published: December 2021

Half-squat parachuting landing is a kind of activity with high impact force. Injuries on lower-extremity joints are common in half-squat parachuting landing and would be increased with a backpack. An ankle brace was used to prevent ankle injuries in landing. However, few quantitative studies reported about the protection of an ankle brace for lower-extremity joints in half-squat parachuting landing with a backpack. This study focused on evaluating the protective effects of an ankle brace in half-squat parachuting landing with a backpack. Seven male participants landed from 120 cm with a backpack and an ankle brace. Each participant performed three landing trials on every experimental condition. Kinetics and kinematics of the hip, knee, and ankle were analyzed. It was found that the ankle brace did not significantly affect the ground reaction force with backpack but increased the ground reaction force from 14.7 ± 2.0 bodyweight to 16.2 ± 1.9 bodyweight (p = 0.017) without the backpack. The ankle brace significantly (p < 0.05) decreased the angular displacement, angular velocity, and angular acceleration of the ankle both without and with the backpack. In conclusion, the ankle brace could restrict ankle motion and significantly increase ground reaction force without the backpack. However, the ankle brace did not significantly influence ground reaction force and still restricted ankle motion with the backpack. Therefore, the ankle brace was more effective in half-squat parachuting landing with the backpack than no-backpack landing.
Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs
Authors: Blanco-Llamero Cristina , García-García Paz , Señoráns Francisco Javier
Published: December 2021

Carrier-free immobilization is a key process to develop efficient biocatalysts able to catalyze the cell wall degradation in microalgae where the traditional solid supports cannot penetrate. Thus, the insolubilization of commercial Celluclast®, Alcalase®, and Viscozyme® enzymes by carrier-free immobilization and their application in microalgae pretreatment was investigated. In this study, different precipitants at different ratios (ethanol, acetone, and polyethylene glycol 4000) were tested in the first part of the method, to establish the precipitation conditions. The screening of the best precipitant is needed as it depends on the nature of the enzyme. The best results were studied in terms of immobilization yield, thermal stability, and residual activity and were analyzed using scanning electron microscopy. Moreover, a novel strategy was intended including the three enzymes (combi-CLEAs) to catalyze the enzymatic degradation of Nannochloropsis gaditana microalgal cell wall in one pot. The carrier-free immobilized derivatives were 10 times more stable compared to soluble enzymes under the same. At the best conditions showed its usefulness in the pretreatment of microalgae combined with ultrasounds, facilitating the cell disruption and lipid recovery. The results obtained suggested the powerful application of these robust biocatalysts with great catalytic properties on novel and sustainable biomass such as microalgae to achieve cost-effective and green process to extract valuable bioactive compounds.
Integrating Artificial Urban Wetlands Into Communities: A Pathway to Carbon Zero?
Authors: Rogerson Robert J. , Horgan Donagh , Roberts Jennifer J.
Published: December 2021

In their natural state, wetland ecosystems provide an optimum natural environment for the sequestration and long-term storage of carbon dioxide (CO2) from the atmosphere. The loss of wetlands under advancing urbanization not only diminishes this capacity for storage, but increases methane and greenhouse gases as the land is disturbed. Nevertheless, there is growing scientific interest in using artificial or constructed wetlands as a way to mitigate the impact of global climate change, with most attention on their use for water management. Using a potential integrated urban wetland site in Glasgow as a case study, this paper critically examines how artificial urban wetlands can contribute to urban net zero targets in terms of their ability for carbon sequestration, and as part of sustainability initiatives more broadly. We find there are several barriers to implementing artificial urban wetlands for carbon drawdown alone, in particular regarding land ownership constraints, uncertainties in capture efficacy and capture quantitation, and eligibility for market-based crediting schemes. These issues make it currently challenging for the carbon reduction contribution of urban wetlands to be quantified and, say, certified to generate revenue to communities through market-based carbon crediting. However, if integrated within wider community-based sustainability initiatives, artificial urban wetlands can support multiple dimensions of sustainability, creating or supporting value far beyond water management and carbon sequestration objectives. Potential co-benefits range from areas such as health and wellbeing, biodiversity, education, food security, behavioural changes, and social care. Our findings show that for these co-benefits to be identified, maximised and realised, a place-based approach to urban wetland development must be adopted, engaging stakeholders from the project outset to define and facilitate collaboration towards shared outcomes for society, community, and environment. These findings will be relevant to any urban infrastructure development seeking to meet sustainability goals beyond carbon capture.
Overcoming Barriers to Injectable Therapies: Development of the ORBIT Intervention Within a Behavioural Change Framework
Authors: McGuigan Karen , Hill Alyson , McCay Deirdre , O’Kane Maurice , Coates Vivien
Published: December 2021

It is estimated among individuals with type 2 diabetes (T2D) requiring injectable therapies to achieve optimal glycaemic control, one-third are reluctant to initiate therapies, with approximately 80% choosing to discontinue or interrupt injectable regimens soon after commencement. Initiation of injectables is a complex issue, with effectiveness of such treatments undermined by non-adherence or poor engagement. Poor engagement and adherence are attributed to psychological aspects such as individuals’ negative perceptions of injectables, depression, anxiety, feelings of shame, distress and perceived lack of control over their condition. The aim of this study was to describe the development of a structured diabetes intervention to address psychological barriers to injectable treatments among a cohort of those with T2D; conducted within a behavioural change framework. An evidence base was developed to inform on key psychological barriers to injectable therapies. A systematic review highlighted the need for theory-based, structured diabetes education focussed on associated psychological constructs to inform effective, patient-centric provisions to improve injectable initiation and persistence. Findings from the focus groups with individuals who had recently commenced injectable therapies, identified patient-centric barriers to initiation and persistence with injectables. Findings from the systematic review and focus groups were translated via Behavioural Change Wheel (BCW) framework to develop an intervention for people with T2D transitioning to injectable therapies: Overcoming and Removing Barriers to Injectable Treatment in T2D (ORBIT). This article describes how psychological barriers informed the intervention with these mapped onto relevant components, intervention functions and selected behaviour change techniques, and finally aligned with behaviour change techniques. This article outlines the systematic approach to intervention development within the BCW framework; guiding readers through the practical application of each stage. The use of the BCW framework has ensured the development of the intervention is theory driven, with the research able to be evaluated and validated through replication due to the clarity around processes and tasks completed at each stage.
Organic Cocrystals: Recent Advances and Perspectives for Electronic and Magnetic Applications
Authors: Jiang Mengjia , Zhen Chun , Li Shuyu , Zhang Xiaotao , Hu Wenping
Published: December 2021

Cocrystal engineering is an advanced supramolecular strategy that has attracted a lot of research interest. Many studies on cocrystals in various application fields have been reported, with a particular focus on the optoelectronics field. However, few articles have combined and summarized the electronic and magnetic properties of cocrystals. In this review, we first introduce the growth methods that serve as the basis for realizing the different properties of cocrystals. Thereafter, we present an overview of cocrystal applications in electronic and magnetic fields. Some functional devices based on cocrystals are also introduced. We hope that this review will provide researchers with a more comprehensive understanding of the latest progress and prospects of cocrystals in electronic and magnetic fields.
Calcium Glycerolate Catalyst Derived from Eggshell Waste for Cyclopentadecanolide Synthesis
Authors: Cheng Haijun , Wei Jiangli , Liang Min , Dai Suyi , Liu Xiongmin , Ma Li , Wang Hongyun , Lai Fang
Published: December 2021

Abstract: The synthesis costs of macrolide musks are higher than those of other commercial musks. To make this process less expensive, eggshell waste was calcined at a low temperature to obtain a catalyst for the cyclopentadecanolide production via reactive distillation using a glycerol entrainer. X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy analyses of the original and recovered catalysts revealed that the main catalytic ingredient was calcium glycerolate (CaG) and not calcium diglyceroxide (CaDG). The basic strengths of CaG and CaDG obtained by Hammett indicators were 7.2 < H_≤ 15.0 and 9.8 < H_≤15.0, while the corresponding base amounts were 1.9 and 7.3 mmol/ g, respectively. Because CaG was soluble in glycerine, the catalyst was efficiently reused. The reaction product containing over 95.0% cyclopentadecanolide with a yield of 49.8% was obtained at a temperature of 190°C and catalyst amount of 12 wt% after 7 h of reaction. Thus, eggshell waste may be directly placed into the reaction mixture after calcination at 600°C to synthesise a large amount of cyclopentadecanolide within a relatively short time. The results of this work indicate that eggshell waste can serve as a potential eco-friendly and affordable catalyst source for the production of macrolide musks.
Novel Extraction Method for Combined Lipid and Metal Speciation From Caenorhabditis elegans With Focus on Iron Redox Status and Lipid Profiling
Authors: Blume Bastian , Witting Michael , Schmitt-Kopplin Philippe , Michalke Bernhard
Published: December 2021

Parkinson´s disease progression is linked to iron redox status homeostasis via reactive oxygen species (ROS) formation, and lipids are the primary targets of ROS. The determination of iron redox status in vivo is challenging and requires specific extraction methods, which are so far tedious and very time-consuming. We demonstrated a novel, faster, and less laborious extraction method using the chelator ethylene glycol l-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid (EGTA) as a stabilizing agent and synthetic quartz beads for homogenization under an argon atmosphere. Additionally, we combined the metal extraction with a well-established lipid extraction protocol using methyl-tert-butyl ether (MTBE) to avoid the problems of lipid precipitation in frozen samples and to determine lipid profiles and metal species from the same batch. The nonextractable matrix, such as the debris, is removed by centrifugation and digested to determine the total metal content of the sample as well. Lipid profiling using RP-LC–MS demonstrated high accordance of the modified extraction method to the reference method, and the organic solvent does not affect the iron redox status equilibrium. Furthermore, rigorous testing demonstrated the stability of the iron redox status equilibrium during the extraction process, secured by complexation, inert atmosphere, fast preparation, and immediately deep frozen extracts.
Core-Shell-Like Structured Co3O4@SiO2 Catalyst for Highly Efficient Catalytic Elimination of Ozone
Authors: Ding Jingya , Cheng Feng , Meng Zhen , Cao Yan , Han Fennv , Chen Dongbin , Cao Mingxiang , Zhang Guolin , Kang Jiahao , Xu Shuxiang , Xu Qi
Published: December 2021

Co3O4 is an environmental catalyst that can effectively decompose ozone, but is strongly affected by water vapor. In this study, Co3O4@SiO2 catalysts with a core-shell-like structure were synthesized following the hydrothermal method. At 60% relative humidity and a space velocity of 720,000 h−1, the prepared Co3O4@SiO2 obtained 95% ozone decomposition for 40 ppm ozone after 6 h, which far outperformed that of the 25wt% Co3O4/SiO2 catalysts. The superiority of Co3O4@SiO2 is ascribed to its core@shell structure, in which Co3O4 is wrapped inside the SiO2 shell structure to avoid air exposure. This research provides important guidance for the high humidity resistance of catalysts for ozone decomposition.
Circadian Rhythms in Bacterial Sepsis Pathology: What We Know and What We Should Know
Authors: Mul Fedele Malena Lis , Senna Camila Agustina , Aiello Ignacio , Golombek Diego Andres , Paladino Natalia
Published: December 2021

Sepsis is a syndrome caused by a deregulated host response to infection, representing the primary cause of death from infection. In animal models, the mortality rate is strongly dependent on the time of sepsis induction, suggesting a main role of the circadian system. In patients undergoing sepsis, deregulated circadian rhythms have also been reported. Here we review data related to the timing of sepsis induction to further understand the different outcomes observed both in patients and in animal models. The magnitude of immune activation as well as the hypothermic response correlated with the time of the worst prognosis. The different outcomes seem to be dependent on the expression of the clock gene Bmal1 in the liver and in myeloid immune cells. The understanding of the role of the circadian system in sepsis pathology could be an important tool to improve patient therapies.
Deficiency of GPI Glycan Modification by Ethanolamine Phosphate Results in Increased Adhesion and Immune Resistance of Aspergillus fumigatus
Authors: Ouyang Haomiao , Zhang Yi , Zhou Hui , Ma Yubo , Li Ruoyu , Yang Jinghua , Wang Xiaowen , Jin Cheng
Published: December 2021

Glycosylphosphatidylinositol (GPI)-anchored proteins play important roles in maintaining the function of the cell wall and participating in pathogenic processes. The addition and removal of phosphoethanolamine (EtN-P) on the second mannose residue in the GPI anchor are vital for maturation and sorting of GPI-anchored proteins. Previously, we have shown that deletion of the gpi7, the gene that encodes an EtN-P transferase responsible for the addition of EtN-P to the second mannose residue of the GPI anchor, leads to the mislocalization of GPI-anchored proteins, abnormal polarity, reduced conidiation, and fast germination in Aspergillus fumigatus. In this report, the adherence and virulence of the A. fumigatus gpi7 deletion mutant were further investigated. The germinating conidia of the mutant exhibited an increased adhesion and a higher exposure of cell wall polysaccharides. Although the virulence was not affected, an increased adherence and a stronger inflammation response of the mutant were documented in an immunocompromised mouse model. An in vitro assay confirmed that the Δgpi7 mutant induced a stronger immune response and was more resistant to killing. Our findings, for the first time, demonstrate that in A. fumigatus, GPI anchoring is required for proper organization of the conidial cell wall. The lack of Gpi7 leads to fast germination, stronger immune response, and resistance to macrophage killing.
Analysis of lncRNAs and mRNA Expression in the ZBTB1 Knockout Monoclonal EL4 Cell Line and Combined Analysis With miRNAs and circRNAs
Authors: Wang Junhong , Li Xiaoxu , Lu Yiyuan , Huang Quntao , Sun Yu , Cheng Mingyang , Li Fengdi , Shi Chunwei , Zeng Yan , Wang Chunfeng , Cao Xin
Published: December 2021

In previous experiments, we identified the effect of deletion of the Zbtb1 gene on circRNAs and microRNAs. In this study, we examined the expression profiles of lncRNAs and mRNAs using the RNA-seq method for Zbtb1-deficient EL4 cells and performed a clustering analysis of differentially expressed lncRNAs and mRNAs. GO term histograms and KEGG scatter plots were drawn. For the experimental results, a joint analysis was performed, which predicted the regulatory relationships among lncRNAs, mRNAs, microRNAs and circRNAs. For the regulatory relationship between lncRNAs and target genes, the chromatin structure and the degree of openness were verified for the possible target gene locations regulated by lncRNA using experimental methods such as Hi-C and ATAC-seq. Ultimately, the possible differential regulation of the Brcal and Dennd5d genes by lncRNAs and the differential changes in transcription factor binding sites in the promoter region were identified. For neRNA-regulated target genes with significantly differentially expressed mRNAs, a combined screen was performed, and the final obtained candidate target genes were subjected to GO and KEGG term enrichment analyses. Our results illustrate that the Zbtb1 gene can not only function as a regulatory factor but also regulate EL4 cells from multiple perspectives based on ceRNA theory.
Segmentation of Tricuspid Valve Leaflets From Transthoracic 3D Echocardiograms of Children With Hypoplastic Left Heart Syndrome Using Deep Learning
Authors: Herz Christian , Pace Danielle F. , Nam Hannah H. , Lasso Andras , Dinh Patrick , Flynn Maura , Cianciulli Alana , Golland Polina , Jolley Matthew A.
Published: December 2021

Hypoplastic left heart syndrome (HLHS) is a severe congenital heart defect in which the right ventricle and associated tricuspid valve (TV) alone support the circulation. TV failure is thus associated with heart failure, and the outcome of TV valve repair are currently poor. 3D echocardiography (3DE) can generate high-quality images of the valve, but segmentation is necessary for precise modeling and quantification. There is currently no robust methodology for rapid TV segmentation, limiting the clinical application of these technologies to this challenging population. We utilized a Fully Convolutional Network (FCN) to segment tricuspid valves from transthoracic 3DE. We trained on 133 3DE image-segmentation pairs and validated on 28 images. We then assessed the effect of varying inputs to the FCN using Mean Boundary Distance (MBD) and Dice Similarity Coefficient (DSC). The FCN with the input of an annular curve achieved a median DSC of 0.86 [IQR: 0.81–0.88] and MBD of 0.35 [0.23–0.4] mm for the merged segmentation and an average DSC of 0.77 [0.73–0.81] and MBD of 0.6 [0.44–0.74] mm for individual TV leaflet segmentation. The addition of commissural landmarks improved individual leaflet segmentation accuracy to an MBD of 0.38 [0.3–0.46] mm. FCN-based segmentation of the tricuspid valve from transthoracic 3DE is feasible and accurate. The addition of an annular curve and commissural landmarks improved the quality of the segmentations with MBD and DSC within the range of human inter-user variability. Fast and accurate FCN-based segmentation of the tricuspid valve in HLHS may enable rapid modeling and quantification, which in the future may inform surgical planning. We are now working to deploy this network for public use.
Single-Cell RNA Sequencing of the Rat Carotid Arteries Uncovers Potential Cellular Targets of Neointimal Hyperplasia
Authors: Gao Xiao-Fei , Chen Ai-Qun , Wang Zhi-Mei , Wang Feng , Luo Shuai , Chen Si-Yu , Gu Yue , Kong Xiang-Quan , Zuo Guang-Feng , Chen Yan , Ge Zhen , Zhang Jun-Jie , Chen Shao-Liang
Published: December 2021

Aims: In-stent restenosis (ISR) remains an Achilles heel of drug-eluting stents despite technical advances in devices and procedural techniques. Neointimal hyperplasia (NIH) is the most important pathophysiological process of ISR. The present study mapped normal arteries and stenotic arteries to uncover potential cellular targets of neointimal hyperplasia.

Methods and Results: By comparing the left (control) and right (balloon injury) carotid arteries of rats, we mapped 11 clusters in normal arteries and 11 mutual clusters in both the control and experimental groups. Different clusters were categorized into 6 cell types, including vascular smooth muscle cells (VSMCs), fibroblasts, endothelial cells (ECs), macrophages, unknown cells and others. An abnormal cell type expressing both VSMC and fibroblast markers at the same time was termed a transitional cell via pseudotime analysis. Due to the high proportion of VSMCs, we divided them into 6 clusters and analyzed their relationship with VSMC phenotype switching. Moreover, N-myristoyltransferase 1 (NMT1) was verified as a credible VSMC synthetic phenotype marker. Finally, we proposed several novel target genes by disease susceptibility gene analysis, such as Cyp7a1 and Cdk4, which should be validated in future studies.

Conclusion: Maps of the heterogeneous cellular landscape in the carotid artery were defined by single-cell RNA sequencing and revealed several cell types with their internal relations in the ISR model. This study highlights the crucial role of VSMC phenotype switching in the progression of neointimal hyperplasia and provides clues regarding the underlying mechanism of NIH.
Macrophage Inflammatory Protein-1 Alpha, a Potential Biomarker for Predicting Left Atrial Remodeling in Patients With Atrial Fibrillation
Authors: Chen Yung-Lung , Wang Hui-Ting , Lin Pei-Ting , Chuang Jiin-Haur , Yang Ming-Yu
Published: December 2021

Objectives:Left atrial (LA) remodeling itself is an independent risk factor for ischemic stroke and mortality, with or without atrial fibrillation (AF). Macrophage inflammatory protein-1 alpha (MIP-1α) has been reported to be involved in the induction of autoimmune myocarditis and dilated cardiomyopathy. Little is known about whether MIP-1α can be used to predict LA remodeling, especially in patients with AF.

Methods:We prospectively enrolled 78 patients who had received a cardiac implantable electronic device due to sick sinus syndrome in order to define AF accurately. AF was diagnosed clinically before enrollment, according to 12-lead electrocardiography (ECG) and 24-h Holter test in 54 (69%) patients. The serum cytokine levels and the mRNA expression levels of peripheral blood leukocytes were checked and echocardiographic study was performed on the same day within 1 week after the patients were enrolled into the study. The 12-lead ECG and 24-h Holter test were performed on the same day of the patients' enrollment, and the device interrogation was performed every 3 months after enrollment. The enrolled patients were clinically followed up for 1 year.

Results:There was no difference in baseline characteristics, cytokine levels and mRNA expression between patients with and without AF. Larger LA volume was positively correlated with higher levels of MIP-1α (r= 0.461,p≤ 0.001) and the atrial high-rate episodes (AHREs) burden (r= 0.593,p< 0.001), and negatively correlated with higher levels of transforming growth factor (TGF)-β1 (r= −0.271,p= 0.047) and TGF-β3 (r= −0.279,p= 0.041). The higher AHREs burden and MIP-1α level could predict LA volume independently. The mRNA expression ofRORCwas negatively associated with the MIP-1α level.

Conclusions:This study showed that higher MIP-1α was significantly associated with LA remodeling and may have the potentials to predict LA remodeling in terms of a larger LA volume, and that circadian gene derangement might affect the expression of MIP-1α.
A Review on the Application of Molecular Dynamics to the Study of Coalbed Methane Geology
Authors: Yu Song , Bo Jiang , Chongtao Wei , Xuguang Dai , Fangkai Quan , Chenliang Hou , Guoxi Cheng
Published: December 2021

Over the last three decades, molecular dynamics (MD) has been extensively utilized in the field of coalbed methane geology. These uses include but are not limited to 1) adsorption of gaseous molecules onto coal, 2) diffusion of gaseous molecules into coal, 3) gas adsorption-induced coal matrix swelling and shrinkage, and 4) coal pyrolysis and combustion. With the development of computation power, we are entering a period where MD can be widely used for the above higher level applications. Here, the application of MD for coalbed methane study was reviewed. Combining GCMC (grand canonical Monte Carlo) and MD simulation can provide microscopic understanding of the adsorption of gaseous molecules onto coal. The experimental observations face significant challenges when encountering the nanoscale diffusion process due to coal structure heterogeneity. Today, all types of diffusion coefficients, such as self-, corrected-, and transport-diffusion coefficients can be calculated based on MD and the Peng-Robinson equation. To date, the MD simulation for both pure and multi-components has reached a situation of unprecedented success. Meanwhile, the swelling deformation of coal has been attracting an increasing amount of attention both via experimental and mimetic angles, which can be successfully clarified using MD and a poromechanical model incorporating the geothermal gradient law. With the development of computational power and physical examination level, simulation sophistication and improvements in MD, GCMC, and other numerical models will provide more opportunities to go beyond the current informed approach, gaining researcher confidence in the engagement in the estimation of coal-swelling deformation behaviors. These reactive MD works have clarified the feasibility and capability of the reactive force field ReaxFF to describe initial reactive events for coal pyrolysis and combustion. In future, advancing MD simulation (primarily characterized by the ReaxFF force field) will allow the exploration of the more complex reaction process. The reaction mechanism of pyrolysis and spontaneous combustion should also be a positive trend, as well as the potential of MD for both visualization and microscopic mechanisms for more clean utilization processes of coal. Thus, it is expected that the availability of MD will continue to increase and be added to the extensive list of advanced analytical approaches to explore the multi-scaled behaviors in coalbed methane geology.
Doushantuo-Pertatataka—Like Acritarchs From the Late Ediacaran Bocaina Formation (Corumbá Group, Brazil)
Authors: Morais L. , Fairchild T. R. , Freitas B. T. , Rudnitzki I. D. , Silva E. P. , Lahr D. , Moreira A. C. , Abrahão Filho E. A. , Leme J. M. , Trindade R. I. F.
Published: December 2021

Acritarchs, a polyphyletic group of acid-resistant organic-walled microfossils, dominate the eukaryotic microfossil record in the Proterozoic (2500–541 Ma) yet exhibit significant reduction in diversity and size at the transition to the Phanerozoic (541–520 Ma). Despite the difficulty of tracing phylogenetic relationships among acritarchs, changes in their complexity and diversity through time have allowed their use in paleoecological and biostratigraphic schemes. The Doushantuo-Pertatataka Ediacaran acritarch assemblage, for example, is usually considered as restricted to the early Ediacaran between 635 and 580 Ma. But similar, diverse acritarchs have been recovered from younger rocks in Mongolia and Arctic Siberia and are now reported here from phosphatized horizons of the upper Bocaina Formation (ca. 555 Ma), Corumbá Group, SW Brazil. In the overlying black limestones and shales of the latest Ediacaran Tamengo Formation (542 Ma) acritarch diversity is low, but the skeletal metazoans Cloudina and Corumbella are abundant. The Bocaina acritarch assemblage shares forms referable to the genera Leiosphaeridia, Tanarium, Asseserium and Megasphaera with the Doushantuo-Pertatataka assemblage, but also includes specimens similar to the Phanerozoic genus Archaeodiscina in addition to two new complex acritarchs. The first is covered by rounded low conical bumps, similar to Eotylotopalla but differs in having a distinct opening suggestive of greater (multicellular?) complexity. The second, identified here as Morphotype 1, is a double-walled acanthomorph acritarch with scattered cylindrical processes between the walls. The contrast in acritarch diversity and abundance between the Bocaina and Tamengo formations is likely due in part to paleoenvironmental and taphonomic differences (absence of the phosphatization window in the latter), as well as to the appearance of both suspension-feeding skeletal metazoans (Cloudina and Corumbella). The occurrence of Doushantuo-Pertatataka acritarchs in SW Brazil, northern Mongolia, and Arctic Siberia extend the biostratigraphic range of this assemblage up to the terminal Ediacaran Cloudina biozone.
A Study of Faculty Retention Factors in Educational Institutes in Context With ABET
Authors: Khan Fazal Qudus , Buhari Seyed M. , Tsaramirsis Georgios , Rasheed Saim
Published: December 2021

A typical higher education institute classically has all the five major constituents, i.e. current students, alumni, faculty members, employers and industrial advisory board. Expectations of the rest of the constituents are fully dependent on the faculty members, as they play a pivotal and backbone role in building up an educational institute, to a high repute. Faculty members provide the knowledge and skills necessary to students, to address market needs. Letting go of skilled, professional and enthusiastic faculty members can have a huge impact on any higher education institution’s reputation and in fulfilling the market needs. In this research, we explore the different influencing factors and criteria for retaining teaching staff at schools and higher education institutes. These criteria are then evaluated against Accreditation Board of Engineering and Technology (ABET) accredited institutes criteria data of “faculty” and “Institutional support,” to see the impact. Study shows that only very few faculty members leave the institution because of higher student-to-faculty ratio or denial of tenure. Also, most of institutions concentrate on providing reduced number of lecture hours per week to keep their faculty members happy and perform research at the institution. Out of 737 faculty members of 27 institutes included in this study, only 0.54% faculty members left ABET accredited institutions.
The Concentration of Selected Inflammatory Cytokines (IL-6, IL-8, CXCL5, IL-33) and Damage-Associated Molecular Patterns (HMGB-1, HSP-70) Released in an Early Response to Distal Forearm Fracture and the Performed Closed Reduction With Kirschner Wire Fixation in Children
Authors: Tylicka Marzena , Guszczyn Tomasz , Maksimowicz Michał , Kamińska Joanna , Matuszczak Ewa , Karpińska Maria , Koper-Lenkiewicz Olga Martyna
Published: December 2021

The evaluation of trauma after surgery through objective analysis of biochemical markers can help in selecting the most appropriate therapy. Thus the aim of the study was the evaluation of the concentration of selected inflammatory cytokines (IL-6, IL-8, CXCL5, IL-33), C-reactive protein (CRP), and damaged-associated molecular patterns (DAMPs): HMGB-1, HSP-70 in the plasma of children in response to bone fracture and 12-14 hours after subsequent surgery performed by closed reduction with percutaneous Kirschner wire fixation (CRKF). The study will answer the question if the CRFK procedure leads to excessive production of inflammatory and damage markers. Blood samples from 29 children with distal forearm fractures were collected 30 min. before CRKF procedure and 12-14 hours after performance of the procedure. The control group was composed of 17 healthy children. IL-6 and CRP concentrations were analyzed using routinely performed in vitro diagnostics tests; the remaining proteins were analyzed with the use of the ELISA method. Increased values of IL-6, CRP, and HSP-70 represented an early inflammatory response to distal forearm fractures classified as SH-II type according to the Salter-Harris classification system. However, the median CRP concentration was within the reference values not indicative of inflammation. The CRKF procedure may be a good solution for the treatment of bone fractures, as damaged associated molecular patterns – HMGB-1 and HSP-70 – did not significantly differ 12-14 hours after the approach was applied as compared to the control group. Moreover, the increase in IL-6 concentration after the CRKF procedure was 1.5-fold to the level before CRKF, while the increase of this marker in response to the distal forearm fracture was 4.3-fold compared to the control group. Based on this data, it appears reasonable to suggest that the CRKF approach caused less damage and inflammatory response in comparison to the response to the fracture itself.
Association of Antibiotic Alterations in Gut Microbiota With Decreased Osseointegration of an Intramedullary Nail in Mice With and Without Osteomyelitis
Authors: Zhao Xingqi , Zhang Zhaohui , Wang Yiran , Qian Kai , Qin Hanjun , Wan Haoyang , Wang Shihao , Zhu Zhengwen , Yang Siqi , Jiang Nan , Zhang Yifang , Bai Yang , Deng Huimin , Yu Bin
Published: December 2021

Treatment of osteomyelitis requires prolonged antibiotic therapy which significantly alters the gut microbiota. While the influences on bone mass and microstructure have been extensively studied, it is poorly understood what impact the changes in gut microbiota may have on the host response to osseointegration around an intramedullary nail implanted. Here, we explored the influence of gut microbiota on the bone osseointegration process around an implant under two conditions: implantation of an intramedullary nail in the bone marrow cavity and chronic osteomyelitis (CO) induced by Staphylococcus aureus infection. Body weight, hepatorenal functions, serum levels of proinflammatory cytokines were monitored. The composition of gut microbiota was assessed via 16S rRNA sequencing, and the bone condition was analyzed via micro-computed tomography, hematoxylin and eosin staining, Safranin O-fast green and Goldner’s trichrome staining. Osteoblastogenesis and osteoclastogenesis were assessed by detecting tartrate-resistant acid phosphatase and osterix expression. We found that perturbation of gut microbiota (increase in Proteobacteria and decrease in Bacteroidetes) associated with delayed osseointegration and increased levels of proinflammatory cytokines in the serum (p<0.05), lower bone mass (p<0.05), deficient endochondral ossification and bone formation, reduced osteoblastogenesis (p<0.05) and enhanced osteoclastogenesis (p<0.001). Survival rates (p=0.002) and bacterial loads (p=0.0363) in bone differed significantly between the CO and antibiotic-treated CO mice, but cytokines levels, bone mineral density, and bone formation did not differ, likely because of the severely damaged bone structure. In summary, antibiotic treatment perturbed the gut microbiota and significantly interfered with the bone osseointegration around the nail by increasing proinflammatory cytokine levels in circulation, inhibiting osteoblastogenesis, enhancing osteoclastogenesis, and thus leading to higher pathogen colonization as well as higher mortality postinfection. This report of ours is the first to demonstrate antibiotic-induced alterations in the gut microbiota affect bone osseointegration, helping us understand the role of gut microbiota disorders in osteoblastogenesis and osteoclastogenesis following implant insertion with or without infection.
Clinical Characteristics and Long-Term Recombinant Human Growth Hormone Treatment of 18q- Syndrome: A Case Report and Literature Review
Authors: Liu Shanshan , Chen Meiping , Yang Hongbo , Chen Shi , Wang Linjie , Duan Lian , Zhu Huijuan , Pan Hui
Published: December 2021

Background

18q- syndrome is a rare chromosomal disease caused by the deletion of the long arm of chromosome 18. Some cases with 18q- syndrome can be combined with growth hormone deficiency (GHD), but data on the efficacy of recombinant human growth hormone (rhGH) treatment in 18q- syndrome are limited.

Methods

Here, we report one case of 18q- syndrome successfully treated with long-term rhGH supplement. Previously reported cases in the literature are also reviewed to investigate the karyotype–phenotype relationship and their therapeutic response to rhGH.

Results

A 7.9-year-old girl was referred for evaluation for short stature. Physical exam revealed proportionally short stature with a height of 111.10 cm (−3.02 SD score (SDS)), low-set ears, a high-arched palate, a small jaw, webbed neck, widely spaced nipples, long and tapering fingers, and cubitus valgus. Thyroid function test indicated subclinical hypothyroidism. The peak value of growth hormone was 10.26 ng/ml in the levodopa provocation test. Insulin-like growth factor 1 (IGF-1) was 126 ng/ml (57–316 ng/ml). Other laboratory investigations, including complete blood cell count, liver and kidney function, gonadal function, serum adrenocorticotropin levels, and serum cortisol levels, were all within normal ranges. Karyotype analysis showed 46, XX, del (18) (q21). L-Thyroxine replacement and rhGH treatment were initiated and maintained in the following 7 years. At the age of 14.8, her height has reached 159.5 cm with a height SDS increase of 2.82 SDS (from −3.02 SDS to −0.20 SDS). No significant side effects were found during the treatment. The literature review indicated the average rhGH treatment duration of 16 patients was 5.9 ± 3.3 years, and the average height SDS significantly increased from −3.12 ± 0.94 SDS to −1.38 ± 1.29 SDS after the rhGH treatment (p < 0.0001).

Conclusion

The main clinical manifestations of 18q- syndrome include characteristic appearance, intellectual disability, and abnormal genital development. The literature review suggested a significant height benefit for short stature with 18q- syndrome from long-term rhGH treatment.
Air Quality Evaluation and Improvement of China’s Three Major Urban Agglomerations Based on the Modified MetaFrontier Dynamic Slack-Based Measures Model
Authors: Cui Zhe , Yang Feng , Ren Fang-rong , Wei Qizheng , Xi Zhengfeng
Published: December 2021

Urban agglomeration has become a unique form of cities during the rapid development of emerging economies. With the increasing attention on global energy and environmental efficiency, air quality evaluation and pollution control have become important standards to measure the health and orderly development of such agglomerations. Based on panel data of 60 cities in the three major urban agglomerations of Beijing-Tianjin-Hebei (BTH), Yangtze River Delta (YRD), and Pearl River Delta (PRD), this study uses the Modified MetaFrontier Dynamic SBM model to evaluate their air quality over the 5-year period of 2013–2017. The results present that the development level of air pollution prevention and control in China’s three major urban agglomerations is relatively low, and YRD as the most developed area has the worst effect of air pollution prevention and control. The MetaFrontier and Group Frontier Efficiency analysis confirms the conclusion of the cluster analysis that a significant two-level differentiation exists in China’s three urban agglomerations. Moreover, China’s three major urban agglomerations are still in the stage of high energy consumption and high development. Lastly, we point out different recommendations for industrial structure and governance foci of the three major urban agglomerations. Dust prevention technology should be improved to reduce PM2.5 in BTH, desulfurization technology should be enhanced to cut industrial SO2 emissions in YRD, and better emission reduction targets and other targeted measures should be formulated in PRD.
Online Sobriety Communities for Women's Problematic Alcohol Use: A Mini Review of Existing Qualitative and Quantitative Research
Authors: Davey Claire
Published: December 2021

The increase in women's drinking is one of the most prominent trends in alcohol consumption in the UK in recent history, possibly exacerbated by COVID-19 lockdown measures. Higher rates of drinking are associated with substantial economic, health, and social costs. However, women are less likely to seek treatment for Alcohol Use Disorder (AUD) than men and have less successful treatment outcomes from traditional treatment paths, such as 12-step programs and in-patient care. Female heavy drinkers may also experience particular forms of gendered stigma that affect their experiences of addiction and recovery and their desire or ability to access these more “traditional” services. This review provides an overview of existing qualitative and quantitative research regarding online sobriety communities that are predominantly utilised by women, such as non-12-step alcohol online support groups (AOSGs) and temporary abstinence initiatives (TAIs). This is a small—but expanding—body of literature emerging as “sober curiosity” and “mindful drinking” are trending in Western contexts such as the UK, particularly amongst young women who do not identify with traditional, binary recovery language such as “alcoholic” and “addict.” This review highlights the gaps in research and concludes that further research regarding these new treatment pathways, and women's experiences when utilising them, must be conducted to provide more evidence-based options for women who want to address problematic drinking. Public health bodies could also learn more effective strategies from these innovative solutions to reduce alcohol consumption generally.
Public Immunity: Evolutionary Spandrels for Pathway-Amplifying Protective Antibodies
Authors: Sangesland Maya , Lingwood Daniel
Published: December 2021

Humoral immunity is seeded by affinity between the B cell receptor (BCR) and cognate antigen. While the BCR is a chimeric display of diverse antigen engagement solutions, we discuss its functional activity as an ‘innate-like’ immune receptor, wherein genetically hardwired antigen complementarity can serve as reproducible templates for pathway-amplifying otherwise immunologically recessive antibody responses. We propose that the capacity for germline reactivity to new antigen emerged as a set of evolutionary spandrels or coupled traits, which can now be exploited by rational vaccine design to focus humoral immunity upon conventionally immune-subdominant antibody targets. Accordingly, we suggest that evolutionary spandrels account for the necessary but unanticipated antigen reactivity of the germline antibody repertoire.
Progress on Poxvirus E3 Ubiquitin Ligases and Adaptor Proteins
Authors: Cui Haoran , Zhang Yaxian , Zhang Leiliang
Published: December 2021

Poxviruses have evolved a variety of innate immunity evasion mechanisms, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. Based on their functional domains and ubiquitin transfer mechanisms, these poxvirus-encoded E3 ubiquitin ligases and adaptor proteins can be divided into five categories: PRANC, ANK/BC, BBK, P28/RING, and MARCH proteins. Although the substrates of many poxvirus E3 ubiquitin ligases remain to be discovered, most of the identified substrates are components of the innate immune system. In this review, we discuss the current research progress on poxvirus-encoded E3 ubiquitin ligases and adaptor proteins to provide mechanistic insights into the interplay between these viruses and their hosts.
A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response
Authors: Li Xingyu , Dong Zhuo , Liu Yan , Song Weifeng , Pu Jieying , Jiang Guanmin , Wu Yongjian , Liu Lei , Huang Xi
Published: December 2021

Dengue Virus (DENV) infection can cause severe illness such as highly fatality dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Innate immune activation by Nod-like receptors (NLRs) is a critical part of host defense against viral infection. Here, we revealed a key mechanism of NLRP12-mediated regulation in DENV infection. Firstly, NLRP12 expression was inhibited in human macrophage following DENV or other flaviviruses (JEV, YFV, ZIKV) infection. Positive regulatory domain 1 (PRDM1) was induced by DENV or poly(I:C) and suppressed NLRP12 expression, which was dependent on TBK-1/IRF3 and NF-κB signaling pathways. Moreover, NLRP12 inhibited DENV and other flaviviruses (JEV, YFV, ZIKV) replication, which relied on the well-conserved nucleotide binding structures of its NACHT domain. Furthermore, NLRP12 could interact with heat shock protein 90 (HSP90) dependent on its Walker A and Walker B sites. In addition, NLRP12 enhanced the production of type I IFNs (IFN-α/β) and interferon-stimulated genes (ISGs), including IFITM3, TRAIL and Viperin. Inhibition of HSP90 with 17-DMAG impaired the upregulation of type I IFNs and ISGs induced by NLRP12. Taken together, we demonstrated a novel mechanism that NLRP12 exerted anti-viral properties in DENV and other flaviviruses (JEV, YFV, ZIKV) infection, which brings up a potential target for the treatment of DENV infection.
Comparison of Female Genital Tract Cytokine and Microbiota Signatures Induced by Initiation of Intramuscular DMPA and NET-EN Hormonal Contraceptives - a Prospective Cohort Analysis
Authors: Dabee Smritee , Tanko Ramla F. , Brown Bryan P. , Bunjun Rubina , Balle Christina , Feng Colin , Konstantinus Iyaloo N. , Jaumdally Shameem Z. , Onono Maricianah , Nair Gonasagrie , Palanee-Phillips Thesla , Gill Katherine , Baeten Jared M. , Bekker Linda-Gail , Passmore Jo-Ann S. , Heffron Renee , Jaspan Heather B. , Happel Anna-Ursula
Published: December 2021

Background

Cervicovaginal inflammation, bacterial microbiota and hormonal contraceptives all influence sexual and reproductive health. To date, the effects of intramuscular depo-medroxyprogesterone acetate (DMPA-IM) versus injectable norethisterone enanthate (NET-EN) on vaginal microbiota or cytokines have not been compared back-to-back, although in-vitro data suggest that DMPA-IM and NET-EN have different pharmacokinetic and biologic activities. This study aimed at comparing the effects of DMPA-IM versus NET-EN initiation on cervicovaginal cytokines and microbiota in women at high risk for sexually transmitted infections (STIs) assigned to the respective contraceptives.

Methods

We collected socio-demographic characteristics and vaginal samples from women initiating DMPA-IM (ECHO Trial; n = 53) and NET-EN (UChoose Trial; n = 44) at baseline and after two consecutive injections to assess cytokine concentrations by Luminex, vaginal microbiota by 16S rRNA gene sequencing, STIs, bacterial vaginosis (BV) and candidiasis.

Results

Cytokine concentrations did not change significantly after initiating DMPA-IM or NET-EN, although NET-EN versus DMPA-IM-associated profiles were distinct. While the abundance of bacterial taxa associated with optimal and non-optimal microbiota fluctuated with DMPA-IM use, overall community composition did not significantly change with either contraceptive. HSV-2 serology, chlamydial infection, gonorrhoea and candidiasis did not influence the associations between contraceptive type and cervicovaginal cytokines or microbiota.

Conclusions

Both DMPA-IM and NET-EN use did not lead to broad inflammatory or microbiota changes in the female genital tract of sub-Saharan African women. This suggests that NET-EN is likely a viable option for contraception in African women at high risk of BV and STIs.