bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomass Quantity and Quality from Different Year-Round Cereal–Legume Cropping Systems as Forage or Fodder for Livestock
Authors: Manoj Konapura Nagaraja , Shekara Bommalapura Gundanaik , Sridhara Shankarappa , Jha Prakash Kumar , Prasad P. V. Vara
Published: August 2021

The quantity and quality of forage and fodder crops is the major drawback of the livestock sector in the country. There is a need to bridge the gap between the supply and demand of fodder through the adoption of specific sustainable fodder production strategies. The field experiments were conducted during kharif (rainy, June–October), rabi (post-rainy, October–February), and summer (March–May) seasons of 2018–19 and 2019–20 to identify a sustainable fodder cropping system module in randomized complete block design with fifteen fodder cropping systems in three replications. The main objective of this research was to identify the most productive cereal–legume cropping system, both in terms of quantity and quality of biomass, to reduce the gap between supply and demand of quality livestock feed around the year. Among cropping systems, Bajra–Napier hybrid intercropped with lucerne, cowpea, and sesbania recorded significantly higher green fodder (163.6, 155.2, and 144.0 t/ha/year, respectively) and dry matter yields (32.1, 30.8, and 31.3 t/ha/year, respectively). Similarly, the same perennial systems also recorded higher quality yield and ash content. However, higher crude protein content was noticed in monocrop legumes, with the highest in sesbania (22.32%), while higher ether extractable fat was found in monocrop sesbania (3.78%). The monocrop oats recorded higher non-fiber carbohydrates (36.90%) while a monocrop of pearl millet recorded higher total carbohydrates (80.75%), however they were on par with other monocrop cereal cropping systems. Cultivation of legumes as a monocrop, and their inclusion as an intercrop with cereals resulted in lower fiber fractions and improved crude protein in intercropping systems. Furthermore, this improved the dry matter intake and digestibility of fodder. With higher sustainable yield index values and land-use efficiency, perennial intercropping systems were also found to be sustainable. Thus, cultivation of the Bajra–Napier hybrid with either lucerne, cowpea, or sesbania as an intercrop will help livestock farmers to achieve higher productivity in terms of quantity and quality, and forms a viable option for overcoming livestock feed scarcity.
Analysis of the Scientific Evolution of the Circular Economy Applied to Construction and Demolition Waste
Authors: Díaz-López Carmen , Bonoli Alessandra , Martín-Morales María , Zamorano Montserrat
Published: August 2021

The circular economy (CE) concept applied to construction and demolition waste (CDW) is a developing field of research that entails a large amount of complex and disjointed information; thus, a comprehensive review of scientific contributions could provide a completed perspective. This article aims to analyse the existing knowledge of CE research applied to CDW, using a double integrated analysis, a systematic literature review and a bibliometric analysis. For this purpose, Science Mapping Analysis Tool (SciMAT), a software for the analysis of performance indicators and visualisations of scientific maps, has been used, which offers a complete approach to the field and evaluates the most cited and productive authors and subject areas related to this discipline. The results obtained from the 1440 bibliographic records from 1993 to 2020 show a still-developing scientific field, evolving from concerns about economic aspects to the most recent progresses in the evaluation of sustainable deconstruction. This work will contribute to the existing body of knowledge by establishing connections, mapping networks of researchers and recommending new trends.
Arctic Futures–Future Arctics?
Authors: Young Oran
Published: August 2021

Is the Arctic sufficiently distinctive and uniform to justify adopting a holistic perspective in thinking about the future of the region? Or do we need to acknowledge that the Arctic encompasses a number of different subregions whose futures may diverge more or less profoundly? In the aftermath of the Cold War, a view of the Arctic as a distinctive region with a policy agenda of its own arose in many quarters and played a prominent role in shaping initiatives such as the launching of the Arctic Environmental Protection Strategy in 1991 and the creation of the Arctic Council in 1996. Yet not everyone found this perspective persuasive at the time, and more recent developments have raised new questions about the usefulness of this perspective as a basis for thinking about the future of the Arctic. As a result, some observers take the view that we need to think more about future Arctics than about Arctic futures. Yet, today, climate change provides a central thread tying together multiple perspectives on the Arctic. The dramatic onset of climate change has turned the Arctic into the frontline with regard to the challenges of adapting to a changing biophysical setting. Ironically, the impacts of climate change also have increased the accessibility of massive reserves of hydrocarbons located in the Arctic, contributing to a feedback loop accelerating climate change. This means that the future of the Arctic will reflect the interplay between efforts to address the biophysical and socioeconomic consequences of climate change on the one hand and the influence of the driving forces underlying the political economy of energy development on the other.
Innovative Olive Tree Leaves Shredder Prototype for the Valorization of Wasted Leaves: An Application to High-Quality Compost Production
Authors: Cappelli Alessio , Menditto Nicola , Cini Enrico
Published: August 2021

Extra virgin olive oil is considered worldwide as one of the most important products, a standard bearer of the Mediterranean diet. Despite this, the production chain of extra virgin olive oil generates four times more waste than quantity of oil. For this reason, the disposal of olive mill wastes represents a significant environmental problem in all the Mediterranean countries. In this direction, several innovations and improvement strategies were proposed in the literature to correctly manage these by-products and, in some cases, to valorize them by the recovery of polyphenols and other interesting substances. However, innovations and improvement strategies for the valorization of olive tree leaves are definitely neglected in the literature, thus motivating this work. The aims of this work are as follow: firstly, to develop and test an innovative olive tree leaves shredder prototype to help olive oil millers in the management of waste leaves deriving from pruning and olive oil production; secondly, to test the combination of the minced olive tree leaves with other by-products in the production of high-quality compost. The results showed the effectiveness of the tested olive tree leaves shredder in significantly reducing the volume occupied by the leaves by 40%. Moreover, the mixing of the minced olive tree leaves with other by-products lead to a high-quality compost which, in accordance with Italian legislation, could even be certified and labeled with this denomination. Future research will investigate the quantification of the benefits in terms of environmental impacts using life cycle assessment.
Investigating the Association between Farmers’ Organizational Participation and Types of Agricultural Product Certifications: Empirical Evidence from a National Farm Households Survey in Taiwan
Authors: Kuan Mei-Yin , Wang Szu-Yung , Wang Jiun-Hao
Published: August 2021

Agricultural product certifications have proliferated due to the growing concerns in many countries over food safety and environmental sustainability. Encouraging farmers to self-organize was regarded as a useful tool to enhance the adoption of agricultural product certifications in Taiwan. However, previous studies solely focused on the association between membership in a production organization and single food certifications. Moreover, little is known of how different types of organizational participation could improve the adoption of agricultural product certifications. This study seeks to fill this knowledge gap by employing multinomial logistic regression model (MNL) to investigate factors affecting farmers’ decisions to participate in agricultural product certification. Special attention is paid to the role of different types of organizational participation in farmers’ choices for agricultural product certification. The study used a nationally representative sample of core farmers in Taiwan, and its results revealed evident differences in farmers’ organization types. For example, the farm operators who participate in agricultural cooperatives (co-ops) tend to use organic labels. In contrast, farm operators who participate in agricultural production and marketing groups (APMGs) tend to adopt the Traceable Agricultural Products (TAP) label. Moreover, age, education level, farming experience, farm labor, farm type, agricultural facilities, and regional location have a significant effect on farmers’ choices for participating in agricultural product certification across different models. The findings suggest that policymakers should consider these differences in the organizational operation of APMGs and co-ops and provide customized measures by promoting different types of agricultural product certifications.
An Auction Bidding Approach to Balance Performance Bonuses in Vehicle Routing Problems with Time Windows
Authors: Cheng Chen-Yang , Ying Kuo-Ching , Lu Chung-Cheng , Yuangyai Chumpol , Chiang Wan-Chen
Published: August 2021

In the field of operations research, the vehicle routing problem with time windows (VRPTW) has been widely studied because it is extensively used in practical applications. Real-life situations discussed in the relevant research include time windows and vehicle capabilities. Among the constraints in a VRPTW, the practical consideration of the fairness of drivers’ performance bonuses has seldom been discussed in the literature. However, the shortest routes and balanced performance bonuses for all sales drivers are usually in conflict. To balance the bonuses awarded to all drivers, an auction bidding approach was developed to address this practical consideration. The fairness of performance bonuses was considered in the proposed mathematical model. The nearest urgent candidate heuristic used in the auction bidding approach determined the auction price of the sales drivers. The proposed algorithm both achieved a performance bonus balance and planned the shortest route for each driver. To evaluate the performance of the auction bidding approach, several test instances were generated based on VRPTW benchmark data instances. This study also involved sensitivity and scenario analyses to assess the effect of the algorithm’s parameters on the solutions. The results show that the proposed approach efficiently obtained the optimal routes and satisfied the practical concerns in the VRPTW.
Mineral Fertilizers Improves the Quality of Turmeric and Soil
Authors: Jabborova Dilfuza , Sulaymanov Khurshid , Sayyed R. Z. , Alotaibi Saad H. , Enakiev Yuriy , Azimov Abdulahat , Jabbarov Zafarjon , Ansari Mohammad Javed , Fahad Shah , Danish Subhan , Datta Rahul
Published: August 2021

An experiment was carried out to investigate the effects of different mineral fertilizers on mineral contents in turmeric rhizomes and soil enzyme activities and soil properties under field conditions in Uzbekistan. The present study is the first report on the impact of mineral fertilizers in turmeric rhizomes and soil enzymes and soil properties in Uzbekistan. The experiment was carried out with four treatments: T1—Control, T2—N75P50K50 kg/ha, T3—N125P100K100 kg/ha, and T4—N100P75K75 + B3Zn6Fe6 kg/ha. Turmeric rhizomes and soil samples were collected from field experiments at the Surkhandarya scientific experimental station of the vegetable, melon crops and potato research institute, Surkhandarya, Uzbekistan. The data showed that T3—the NPK (125:100:100 kg/ha) and T4—the NPK + BZnFe (100:75:75:3:6:6 kg/ha) treatments significantly enhanced K content by 27–21%, Ca content by 43–38%, and P content by 54–17% in turmeric rhizomes as compared to control without fertilizer. A maximum of turmeric rhizome microelements content was recorded with T4, which also resulted in improved Fe, Zn, Cu, Cr, and Mo contents in turmeric rhizomes and mineral contents of soil compared to other treatments. This treatment significantly enhanced active P content by 34%, active K content by 25%, total P content by 62%, total K content by 14%, and the activities of soil urease, invertase, catalase, and phosphatase over those in the control. The present study results suggest that the application of NPK + BZnFe (100:75:75:3:6:6 kg/ha) improves macro and micronutrient contents in turmeric rhizomes and activities of soil enzymes and physicochemical properties of soil.
Escaping the Climate Trap: Participation in a Climate-Specific Social Dilemma Simulation Boosts Climate-Protective Motivation and Actions
Authors: Druen Perri B. , Zawadzki Stephanie J.
Published: August 2021

One way in which educators can help to address increasingly pressing environmental problems, including climate change, is to encourage individuals to change their behavior and to press for structural changes in society. The promotion of climate-protective behavior is challenging because the payoffs for various actions and inactions are structured such that they create social dilemmas. The conflict between short-term personal benefit and long-term collective gain in such dilemmas often leads to self-serving motives that can ultimately be self-defeating. We created a social dilemma simulation specific to climate change, called Climate Trap: Social Dilemma Simulation, to help students observe how they and others respond to these conflicts, and predicted that doing so would lead to climate-protective motivations and behaviors after the simulation. The simulation participants (N = 344) reported greater confidence in their knowledge and understanding of the social dilemma context of climate change, higher environmental concern and more self-determined motivation to act, and they engaged in more climate-protective behaviors compared to the students who did not complete the simulation. Moreover, the simulation participants reported greater pro-environmental engagement on all of the measures after the simulation compared to before they participated in the simulation. The results suggest that a climate-specific social dilemma simulation can create uniquely motivating experiences, and can have utility as a teaching tool, research instrument and intervention.
Consumers’ Attitude and Intention towards Organic Fruits and Vegetables: Empirical Study on Romanian Consumers
Authors: Radulescu Violeta , Cetina Iuliana , Cruceru Anca Francisca , Goldbach Dumitru
Published: August 2021

Organic farming is one of the fastest growing sectors, both in developed and developing countries. Although Romania is in sixth place at the level of the European Union in terms of agricultural area, the share of ecologically certified areas is low. The fruits and vegetables sector is the most important for the agri-food production in Romania, accounting for 58% of the total cultivated area. In recent years, there is a tendency for consumers to focus on healthy diets with safe, high quality foods and high nutritional compounds from unpolluted areas. However, the Romanian market of organic fruits and vegetables is still underdeveloped due to the small number of consumers of organic products. The future of organic farming is largely dependent on attitude and consumer demand. The purpose of this paper is to study the attitudes and intentions of Romanian consumers towards the consumption of organic fruits and vegetables through direct research among 268 individuals. The results show that the attitudes of individuals towards the consumption of organic fruits and vegetables is directly and positively influenced by the information that individuals have about the characteristics of organic products, their personal needs and motivations, but also by external influences, and the purchase barriers do not represent elements with a strong enough influence on the attitude and intention.
Quantifying Food Loss and Waste in Saudi Arabia
Authors: Alshabanat Zaid , Alkhorayef Abdulrahman , Ben Haddad Hedi , Mezghani Imed , Gouider Abdessalem , Tlili Adel , Allouche Mohamed. A. , Gannouni Kais A.
Published: August 2021

Using the FAO model calculations proposed by Gustavsson et al. (2013) and FAO (2014), food loss and waste (FLW) is measured in Saudi Arabia with a special focus on wheat, rice, dates, poultry, vegetables, fruits, fish, and meat. Results show that the overall FLW rate is 33.1%, where the food loss rate is 14.2%, and the food waste rate is 18.9%. Acceding to the disaggregated results, we find that FLW rates are distributed as follows: 29.7% for wheat, 33.6% for rice, 21.4%, for dates 29.1% for poultry, 39.5% for vegetables, 39.6% for fruits, 33% for fish, and 31.3% for meat. The Sustainable Development Goal (SDG 12.3) target is to reduce the rates of food loss and waste by 50% in 2030, and to help achieve that goal, we employed a nonlinear optimisation simulation model with the objective function of reducing FLW by 50% over the period 2020–2030. Based on the findings achieved, recommendations are made to cover the various aspects of the whole food supply chain (FSC) and to aim at more efficiency and higher levels of productivity. Our findings have significant implications by estimating the FLW baseline indicator and providing the different stakeholders of FSC with the optimal actions to do to reduce FLW rates.
Product Service System Configuration Based on a PCA-QPSO-SVM Model
Authors: Cui Zhaoyi , Geng Xiuli
Published: August 2021

To achieve sustainable development and improve market competitiveness, many manufacturers are transforming from traditional product manufacturing to service manufacturing. In this trend, the product service system (PSS) has become the mainstream of supply to satisfy customers with individualized products and service combinations. The diversified customer requirements can be realized by the PSS configuration based on modular design. PSS configuration can be deemed as a multi-classification problem. Customer requirements are input, and specific PSS is output. This paper proposes an improved support vector machine (SVM) model optimized by principal component analysis (PCA) and the quantum particle swarm optimization (QPSO) algorithm, which is defined as a PCA-QPSO-SVM model. The model is used to solve the PSS configuration problem. The PCA method is used to reduce the dimension of the customer requirements, and the QPSO is used to optimize the internal parameters of the SVM to improve the prediction accuracy of the SVM classifier. In the case study, a dataset for central air conditioning PSS configuration is used to construct and test the PCA-QPSO-SVM model, and the optimal PSS configuration can be predicted well for specific customer requirements.
Sonic Tomograph as a Tool Supporting the Sustainable Management of Historical Greenery of the UMCS Botanical Garden in Lublin
Authors: Dudkiewicz Margot , Durlak Wojciech
Published: August 2021

The mission of botanical gardens is to work towards the conservation and sustainable use of plant diversity. In the case of gardens established on the premises of former manor estates, actions are also being taken to properly manage existing natural resources, e.g., historic tree stands. This article is based on the case study of the Botanical Garden in Lublin. To assess the health condition of the trees growing in the oldest part of the park—Kościuszko Redoubt—sonic tomography examinations were performed. The article presents the practical application of a newer form of digital imaging, put to the service of assessing the health of old trees in an important public space. The Redoubt is the only work of defense architecture in Poland related to the activity of General Tadeusz Kościuszko, the hero of Poland and the United States of America. It has survived in an excellent condition, preserved without significant changes, as an earth structure with ramparts; an interior, i.e., a yard; a cannon post located on an inaccessible cliff; and a brick basement serving as a shelter for soldiers and a warehouse. The entire surroundings also survived: ravines masked with a wild green, embankments with a safe hidden access road to the Redoubt, and a shaped defense line. The Botanical Garden area is located in the Sławinek district, in the valley of the Czechówka River, and includes a fragment of it and the slope adjacent to the west, cut by three loess ravines. The Botanical Garden was established in 1965, where there was a manor farm earlier, and then in the 19th century, a spa that was destroyed during World War I and II. Good soils and a varied microclimate provide the right ecological conditions for the high biodiversity of plants. With an area of 21.15 ha, the Garden area has the character of a landscape park with the theme of a manor garden from the turn of the 18th/19th century and a spa garden. The primary purpose of the work was to investigate the effectiveness of the use of computer tools in the sustainable management of historical greenery in the Botanical Garden. Research has shown that diagnostics with the use of sound waves allow for an accurate diagnosis and quick protective measures against the tree, improving the safety of visitors to the botanical garden. Moreover, the obtained results and a historical query were used to prepare an application to recognize Redoubt as a monument.
Schrödinger Equations in Electromagnetic Fields: Symmetries and Noncommutative Integration
Authors: Magazev Alexey Anatolievich , Boldyreva Maria Nikolaevna
Published: August 2021

We study symmetry properties and the possibility of exact integration of the time-independent Schrödinger equation in an external electromagnetic field. We present an algorithm for constructing the first-order symmetry algebra and describe its structure in terms of Lie algebra central extensions. Based on the well-known classification of the subalgebras of the algebra e(3), we classify all electromagnetic fields for which the corresponding time-independent Schrödinger equations admit first-order symmetry algebras. Moreover, we select the integrable cases, and for physically interesting electromagnetic fields, we reduced the original Schrödinger equation to an ordinary differential equation using the noncommutative integration method developed by Shapovalov and Shirokov.
Application of Chemical Monitoring and Public Alarm Systems to Reduce Public Vulnerability to Major Accidents Involving Dangerous Substances
Authors: Cimer Zsolt , Vass Gyula , Zsitnyányi Attila , Kátai-Urbán Lajos
Published: August 2021

As a result of economic development and an increase in the volume of industrial production, the use of dangerous substances is increasing despite the fact that most industrial facilities are committed to the principles of environmental protection and sustainable development. Protection of human health and the environment is ensured at the local level by the local safety system. Major accidents typically have an off-site impact that also affects the general public. The most significant asymmetric event is when toxic substances are release into a populated area following a major accident. Early warning systems can significantly reduce the harmful consequences of major accidents that may occur. The operation of a reliable and effective chemical monitoring and public alarm system can be used as a basic device of defence. This ultimately means restoring the symmetry of the local safety system. It was an important scientific objective in Hungary to identify the facilities endangering the population where it is necessary to install chemical monitoring and early warning external protection systems. In this context, the main objective of this study was to present dangerous plant identification methodology and to analyse and evaluate the results of the application of this methodology.
Solutions of the Bullough–Dodd Model of Scalar Field through Jacobi-Type Equations
Authors: Cimpoiasu Rodica , Constantinescu Radu , Pauna Alina Streche
Published: August 2021

A technique based on multiple auxiliary equations is used to investigate the traveling wave solutions of the Bullough–Dodd (BD) model of the scalar field. We place the model in a flat and homogeneous space, considering a symmetry reduction to a 2D-nonlinear equation. It is solved through this refined version of the auxiliary equation technique, and multiparametric solutions are found. The key idea is that the general elliptic equation, considered here as an auxiliary equation, degenerates under some special conditions into subequations involving fewer parameters. Using these subequations, we successfully construct, in a unitary way, a series of solutions for the BD equation, part of them not yet reported. The technique of multiple auxiliary equations could be employed to handle several other types of nonlinear equations, from QFT and from various other scientific areas.
Lie Symmetries and Solutions of Reaction Diffusion Systems Arising in Biomathematics
Authors: Torrisi Mariano , Traciná Rita
Published: August 2021

In this paper, a special subclass of reaction diffusion systems with two arbitrary constitutive functions Γ(v) and H(u,v) is considered in the framework of transformation groups. These systems arise, quite often, as mathematical models, in several biological problems and in population dynamics. By using weak equivalence transformation the principal Lie algebra, LP, is written and the classifying equations obtained. Then the extensions of LP are derived and classified with respect to Γ(v) and H(u,v). Some wide special classes of special solutions are carried out.
A Systematic Review on the Interaction between Emotion and Pseudoneglect
Authors: Strappini Francesca , Galati Gaspare , Pecchinenda Anna
Published: August 2021

Background: A large body of research has shown brain asymmetries in spatial attention. Specifically, there is an attention-processing advantage for the left visual field in healthy, right-handed subjects, known as “pseudoneglect.” Several studies have revealed that emotions modulate this basic spatial phenomenon, but the direction of the effect is still unclear. Here we systematically review empirical evidence on the behavioral effects of emotion on pseudoneglect. Methods: We searched through Pubmed, Scopus, PsycINFO, and PsychArticles. Original peer-reviewed articles published until February 2021 were included if they (1) were written in English; (2) were conducted on adults; (3) included at least one task to measure pseudoneglect, and (4) included at least one task with emotional stimuli or employed a measure of emotional state/trait, as they relate to pseudoneglect. Results: Fifteen studies were included, and 784 healthy participants took part in all studies reviewed. Discussion: The results show some evidence of emotion modulation of pseudoneglect, but evidence on the direction of the effect is mixed. We discuss the role of methodological factors that could account for the available findings and the implications for emotion asymmetry hypotheses such as the right-hemisphere hypothesis, the valence-specific hypothesis, as well as neural and arousal frameworks of attention–emotion interactions.
Shear-Driven Mechanism of Temperature Gradient Formation in Microfluidic Nematic Devices: Theory and Numerical Studies
Authors: Śliwa Izabela , Zakharov Alex
Published: August 2021

The purpose of this paper is to show some routes in describing the mechanism responsible for the formation of the temperature difference ΔT at the boundaries of the microfluidic hybrid aligned nematic (HAN) channel, initially equal to zero, if one sets up the stationary hydrodynamic flow vst or under the effect of an externally applied shear stress (SS) to the bounding surfaces. Calculations based on the nonlinear extension of the classical Ericksen–Leslie theory, supplemented by thermomechanical correction of the SS σzx and Rayleigh dissipation function while accounting for the entropy balance equation, show that the ΔT is proportional to the heat flux q across the HAN channel and grows by up to several degrees under the influence of the externally applied SS. The role of vst=ust(z)i^ with a sharp triangular profile ust(z) across the HAN in the production of the highest ΔT is also investigated.
Coloring Properties of Mixed Cycloids
Authors: Dósa György , Newman Nicholas , Tuza Zsolt , Voloshin Vitaly
Published: August 2021

In this paper, we investigate partitions of highly symmetrical discrete structures called cycloids. In general, a mixed hypergraph has two types of hyperedges. The vertices are colored in such a way that each C-edge has two vertices of the same color, and each D-edge has two vertices of distinct colors. In our case, a mixed cycloid is a mixed hypergraph whose vertices can be arranged in a cyclic order, and every consecutive p vertices form a C-edge, and every consecutive q vertices form a D-edge in the ordering. We completely determine the maximum number of colors that can be used for any p≥3 and any q≥2. We also develop an algorithm that generates a coloring with any number of colors between the minimum and maximum. Finally, we discuss the colorings of mixed cycloids when the maximum number of colors coincides with its upper bound, which is the largest cardinality of a set of vertices containing no C-edge.
Origin and Characterization of Extracellular Vesicles Present in the Spider Venom of Ornithoctonus hainana
Authors: Xun Chengfeng , Wang Lu , Yang Hailin , Xiao Zixuan , Deng Min , Xu Rongfang , Zhou Xi , Chen Ping , Liu Zhonghua
Published: August 2021

Extracellular vesicles (EVs), including exosomes and microvesicles, are membranous vesicles released from nearly all cellular types. They contain various bioactive molecules, and their molecular composition varies depending on their cellular origin. As research into venomous animals has progressed, EVs have been discovered in the venom of snakes and parasitic wasps. Although vesicle secretion in spider venom glands has been observed, these secretory vesicles’ origin and biological properties are unknown. In this study, the origin of the EVs from Ornithoctonus hainana venom was observed using transmission electron microscopy (TEM). The Ornithoctonus hainana venom extracellular vesicles (HN-EVs) were isolated and purified by density gradient centrifugation. HN-EVs possess classic membranous vesicles with a size distribution ranging from 50 to 150 nm and express the arthropod EV marker Tsp29Fb. The LC-MS/MS analysis identified a total of 150 proteins, which were divided into three groups according to their potential function: conservative vesicle transport-related proteins, virulence-related proteins, and other proteins of unknown function. Functionally, HN-EVs have hyaluronidase activity and inhibit the proliferation of human umbilical vein endothelial cells (HUVECs) by affecting the cytoskeleton and cell cycle. Overall, this study investigates the biological characteristics of HN-EVs for the first time and sheds new light on the envenomation process of spider venom.
Botulinum Toxin Services for Neurorehabiliation: Recommendations for Challenges and Opportunities during the COVID-19 Pandemic
Authors: Bavikatte Ganesh , Jacinto Jorge , Deltombe Thierry , Wissel Joerg
Published: August 2021

The COVID-19 pandemic severely impacted the function of medical facilities and rehabilitation services worldwide, including toxin services delivering Botulinum toxin treatments for neuromuscular conditions such as spasticity, dystonia, and sialorrhea. The aim of this paper is to understand how toxin services have dealt with the situation and what strategies have been adopted to continue services. The recommendations are based on a virtual round table held with toxin services experts from different European countries who shared their experiences and discussed the best practices. The challenges for toxin services were reviewed based on the experts’ experiences and on relevant literature from 2020 and 2021. A set of recommendations and best practices were compiled, focusing firstly on guidance for clinical practice, including assessing patients’ health and risk status and the urgency of their treatment. Secondly, it was discussed how patients on botulinum toxin therapy can be cared for and supported during the pandemic, and how modern technology and tele-medicine platforms can be generally used to optimize effectiveness and safety of toxin treatments. The technological advances prompted by the COVID-19 crisis can result in better and more modern patient care in the future.
Longitudinal Secretion of Paramyxovirus RNA in the Urine of Straw-Coloured Fruit Bats (Eidolon helvum)
Authors: Jolma Elli Rosa , Gibson Louise , Suu-Ire Richard D. , Fleischer Grace , Asumah Samuel , Languon Sylvester , Restif Olivier , Wood James L. N. , Cunningham Andrew A.
Published: August 2021

The straw-coloured fruit bat (Eidolon helvum) is widespread in sub-Saharan Africa and is widely hunted for bushmeat. It is known to harbour a range of paramyxoviruses, including rubuloviruses and henipaviruses, but the zoonotic potential of these is unknown. We previously found a diversity of paramyxoviruses within a small, captive colony of E. helvum after it had been closed to contact with other bats for 5 years. In this study, we used under-roost urine collection to further investigate the paramyxovirus diversity and ecology in this colony, which had been closed to the outside for 10 years at the time of sampling. By sampling urine weekly throughout an entire year, we investigated possible seasonal patterns of shedding of virus or viral RNA. Using a generic paramyxovirus L-gene PCR, we detected eight distinct paramyxovirus RNA sequences. Six distinct sequences were detected using a Henipavirus-specific PCR that targeted a different region of the L-gene. Sequence detection had a bi-annual pattern, with the greatest peak in July, although different RNA sequences appeared to have different shedding patterns. No significant associations were detected between sequence detection and birthing season, environmental temperature or humidity, and no signs of illness were detected in any of the bats in the colony during the period of sample collection.
Protective Effects of Astodrimer Sodium 1% Nasal Spray Formulation against SARS-CoV-2 Nasal Challenge in K18-hACE2 Mice
Authors: Paull Jeremy R. A. , Luscombe Carolyn A. , Castellarnau Alex , Heery Graham P. , Bobardt Michael D. , Gallay Philippe A.
Published: August 2021

Strategies to combat COVID-19 require multiple ways to protect vulnerable people from infection. SARS-CoV-2 is an airborne pathogen and the nasal cavity is a primary target of infection. The K18-hACE2 mouse model was used to investigate the anti-SARS-CoV-2 efficacy of astodrimer sodium formulated in a mucoadhesive nasal spray. Animals received astodrimer sodium 1% nasal spray or PBS intranasally, or intranasally and intratracheally, for 7 days, and they were infected intranasally with SARS-CoV-2 after the first product administration on Day 0. Another group was infected intranasally with SARS-CoV-2 that had been pre-incubated with astodrimer sodium 1% nasal spray or PBS for 60 min before the neutralisation of test product activity. Astodrimer sodium 1% significantly reduced the viral genome copies (>99.9%) and the infectious virus (~95%) in the lung and trachea vs. PBS. The pre-incubation of SARS-CoV-2 with astodrimer sodium 1% resulted in a significant reduction in the viral genome copies (>99.9%) and the infectious virus (>99%) in the lung and trachea, and the infectious virus was not detected in the brain or liver. Astodrimer sodium 1% resulted in a significant reduction of viral genome copies in nasal secretions vs. PBS on Day 7 post-infection. A reduction in the viral shedding from the nasal cavity may result in lower virus transmission rates. Viraemia was low or undetectable in animals treated with astodrimer sodium 1% or infected with treated virus, correlating with the lack of detectable viral replication in the liver. Similarly, low virus replication in the nasal cavity after treatment with astodrimer sodium 1% potentially protected the brain from infection. Astodrimer sodium 1% significantly reduced the pro-inflammatory cytokines IL-6, IL-1α, IL-1β, TNFα and TGFβ and the chemokine MCP-1 in the serum, lung and trachea vs. PBS. Astodrimer sodium 1% nasal spray blocked or reduced SARS-CoV-2 replication and its sequelae in K18-hACE2 mice. These data indicate a potential role for the product in preventing SARS-CoV-2 infection or for reducing the severity of COVID-19.
Spatial Prediction of Groundwater Potentiality in Large Semi-Arid and Karstic Mountainous Region Using Machine Learning Models
Authors: Namous Mustapha , Hssaisoune Mohammed , Pradhan Biswajeet , Lee Chang-Wook , Alamri Abdullah , Elaloui Abdenbi , Edahbi Mohamed , Krimissa Samira , Eloudi Hasna , Ouayah Mustapha , Elhimer Hicham , Tagma Tarik
Published: August 2021

The drinking and irrigation water scarcity is a major global issue, particularly in arid and semi-arid zones. In rural areas, groundwater could be used as an alternative and additional water supply source in order to reduce human suffering in terms of water scarcity. In this context, the purpose of the present study is to facilitate groundwater potentiality mapping via spatial-modelling techniques, individual and ensemble machine-learning models. Random forest (RF), logistic regression (LR), decision tree (DT) and artificial neural networks (ANNs) are the main algorithms used in this study. The preparation of groundwater potentiality maps was assembled into 11 ensembles of models. Overall, about 374 groundwater springs was identified and inventoried in the mountain area. The spring inventory data was randomly divided into training (75%) and testing (25%) datasets. Twenty-four groundwater influencing factors (GIFs) were selected based on a multicollinearity test and the information gain calculation. The results of the groundwater potentiality mapping were validated using statistical measures and the receiver operating characteristic curve (ROC) method. Finally, a ranking of the 15 models was achieved with the prioritization rank method using the compound factor (CF) method. The ensembles of models are the most stable and suitable for groundwater potentiality mapping in mountainous aquifers compared to individual models based on success and prediction rate. The most efficient model using the area under the curve validation method is the RF-LR-DT-ANN ensemble of models. Moreover, the results of the prioritization rank indicate that the best models are the RF-DT and RF-LR-DT ensembles of models.
Coupling Water Resources and Agricultural Practices for Sorghum in a Semiarid Environment
Authors: Carvalho Ailton Alves de , Montenegro Abelardo A. de A. , de Lima João L. M. P. , Silva Thieres George Freire da , Pedrosa Elvira Maria Regis , Almeida Thayná Alice Brito
Published: August 2021

Water scarcity and changing rainfall distribution have caused uncertainties in relation to agricultural production in semiarid areas. In this context, water reuse for irrigation is a promising alternative, although requiring irrigation and agricultural management. Production of forage plants is strategic for semiarid areas due to their high tolerance to stresses and use as animal fodder. The objective of this work was to evaluate the combined performance of treated wastewater irrigation and mulching on forage sorghum and on soil attributes in Northeast Brazil. Sorghum was cropped in November 2018, three months before the beginning of the hydrologic year, and cultivated over three cycles until April 2019. The experiment was designed with five irrigation depths (60%, 80%, 100%, 120%, and 140% of crop evapotranspiration), and with four soil cover conditions (0% (witness), soil covered with native vegetation, with coconut coir, and with macerated moringa seeds), adopting four replications. Irrigation with treated wastewater promoted linear increases in forage sorghum yield at irrigation depths of up to 140% of crop evapotranspiration. Mulch promoted a 24% increase in productivity in relation to the area where conservation was not practiced, being able to control salinity while also contributing to the higher incorporation of organic matter. Irrigation with treated wastewater had no negative agronomic impacts on soil, once natural rainfall events typical of the Brazilian semiarid region allowed effective salt leaching from shallow sandy soils.
Influence of Sustainable Biochars Produced from Kitchen Waste, Pig Manure, and Wood on Soil Erosion
Authors: Huang Xilong , Niu Renjie , Huang Xiaoli , An Yongxue , Li Junhao , Li Manqi , Huang He , Garg Ankit
Published: August 2021

The influence of biochars on water retention, mitigating nutrient leaching, and pollutant removal in green infrastructure has been explored in the past. However, there is a lack of understanding on how feedstock (i.e., biomass) would affect biochar physicochemical properties and hence, overall erosion control (including infiltration, surface, and sub-surface runoff) in green infrastructure. The main purpose of this study was to investigate the effect of biochars produced from three different feedstocks (pig manure, wood, and kitchen waste) on the erosion of granite residual soil. Flume experiments were conducted to measure and analyze soil erosion, runoff, and infiltration. The result showed that the runoff and soil erosion of kitchen waste biochar (KWB) samples were reduced by 17.7% and 21.7%, respectively. On the contrary, wood biochar (WB) and pig manure biochar (PMB) were found to enhance runoff and soil erosion. In addition, biochar particles were found in runoff and infiltration in erosion experiment. Thus, it is important to note that measures should be taken to prevent biochar loss when using biochar as a soil amendment. Additionally, the effects of different types of biochar on soil hydraulic and hydrophobicity properties should be taken into account as a selection criterion for choosing amendments in green infrastructure. This study finds that kitchen waste biochar has better performance in improving soil hydraulics and erosion.
Ice Melt-Induced Variations of Structural and Functional Traits of the Aquatic Microbial Community along an Arctic River (Pasvik River, Norway)
Authors: Papale Maria , Rizzo Carmen , Caruso Gabriella , Amalfitano Stefano , Maimone Giovanna , Miserocchi Stefano , La Ferla Rosabruna , Aspholm Paul Eric , Decembrini Franco , Azzaro Filippo , Conte Antonella , Graziano Marco , Rappazzo Alessandro Ciro , Lo Giudice Angelina , Azzaro Maurizio
Published: August 2021

The effects of climate change-induced ice melting on the microbial communities in different glacial-fed aquatic systems have been reported, but seasonal dynamics remain poorly investigated. In this study, the structural and functional traits of the aquatic microbial community were assessed along with the hydrological and biogeochemical variation patterns of the Arctic Pasvik River under riverine and brackish conditions at the beginning (May = Ice-melt (−)) and during the ice-melting season (July = Ice-melt (+)). The microbial abundance and morphometric analysis showed a spatial diversification between the riverine and brackish stations. Results highlighted different levels of microbial respiration and activities with different carbon and phosphorous utilization pathways, thus suggesting an active biogeochemical cycling along the river especially at the beginning of the ice-melting period. At Ice-melt (−), Gammaproteobacteria and Alphaproteobacteria were dominant in riverine and brackish stations, respectively. Conversely, at Ice-melt (+), the microbial community composition was more homogeneously distributed along the river (Gammaproteobacteria > Alphaproteobacteria > Bacteroidetes). Our findings provide evidence on how riverine microbial communities adapt and respond to seasonal ice melting in glacial-fed aquatic ecosystems.
Response of Zooplankton Size Structure to Multiple Stressors in Urban Lakes
Authors: Toruan Reliana Lumban , Coggins Liah X. , Ghadouani Anas
Published: August 2021

Urban lakes are important environmental assets that contribute significant ecosystem services in urbanised areas around the world. Consequently, urban lakes are more exposed to anthropogenic pressures. Zooplankton communities play a central role in lake processes and, as such, are very sensitive to the impacts of human activities both through in-lake and catchment processes. Understanding their ecological function in urban lakes and how they respond to urbanisation is essential for environmental sustainability. In this study, we investigated the reliability of zooplankton size structure as indicators of anthropogenic stressors in urban lakes. We examined the relationship between environmental variables and zooplankton community size spectra derived as mean body size, density, and biomass. Our study showed that the overall mean body size was within the small size group ranged from 416 to 735 µm equivalent spherical diameter (ESD). Despite no significant difference in total zooplankton density between lakes, there was variability in the total density of the five different size classes. Total biomass was characterised by a significant proportion of size >750 µm. As the specific parameter of normalised biomass size spectra (NBSS), the slopes of the NBSS varied from moderate (−0.83 to −1.04) for a community with higher biomass of the larger size zooplankton to steeper slopes (from −1.15 to −1.49) for a community with higher biomass of smaller size. The environmental variables, represented by total phosphorus (TP) and chlorophyll a (chl-a), had a strong effect on zooplankton biomass and NBSS, where TP and chl-a were significantly correlated with the increase of total biomass and corresponded well with a less negative slope. Our results indicated that the community metric was sensitive to nutrient input and that size-based metrics have the potential to serve as key indicators for the management of urban lakes.
Aspects of treatment of isolated systolic arterial hypertension in the elderly in the practice of a family doctor
Authors: Skvortsov Vsevolod Vladimirovich , Skvortsova Ekaterina Mikhailovna
Published: August 2021

This article is devoted to the treatment of isolated systolic arterial hypertension in the elderly, based on the current ESH/ESC recommendations.
Fundamentals of differential diagnosis of hemorrhagic vasculitis
Authors: Klimov Vladimir Anatolievich
Published: August 2021

It is quite rare, but in the practice of a family doctor, a condition may occur when, after an upper respiratory tract infection, flu or tonsillitis, the patient develops petechial rashes, hemorrhages in the mucous membranes, articular syndrome develops and abdominal pain appears. The first elements of the rash appear symmetrically first on the lower extremities, and then spread throughout the body. Joint lesions are characterized by severe pain syndrome and swelling; changes usually affect large joints – knee and ankle, but sometimes smaller articular surfaces are affected. Hemorrhages in the intestinal wall and mesentery are the cause of abdominal pain, flatulence, bowel disorders; nausea and vomiting are possible, sometimes mixed with blood. All these signs are characteristic of hemorrhagic vasculitis, which is also called Henoch–Schonlein purpura, capillary toxicosis or allergic purpura. For the first time, a description of several cases of purpura associated with arthritis was given by a German doctor Johann Schonlein in 1937, and a few decades later this nosology was described in detail by Eduard Henoch in his scientific work. In this pathological condition, autoimmune damage to small arteries is observed, as a result of which they become fragile, their permeability increases, resulting in the development of microthrombosis and multiple small hemorrhages in the skin, mucous membranes and internal organs. The disease most often occurs in children and adolescents, however, cases of diagnosis of hemorrhagic vasculitis have been described in adults as well [7].