bluuebunny/crossref_metadata_2025 huggingface dataset:
A tale of two capitalisms
Authors: Hall Steve , McLean Craig
Published: August 2009

This article examines comparative homicide rates in the United States and Western Europe in an era of increasingly globalized neo-liberal economics. The main finding of this preliminary analysis is that historical and spatial correlations between distinct forms of political economy and homicide rates are consistent enough to suggest that social democratic regimes are more successful at fostering the socio-cultural conditions necessary for reduced homicide rates. Thus Western Europe and all continents and nations should approach the importation of American neo-liberal economic policies with extreme caution. The article concludes by suggesting that the indirect but crucial causal connection between political economy and homicide rates, prematurely pushed into the background of criminological thought during the ‘cultural turn’, should be returned to the foreground.
At the End of the Post-Communist Transformation? Normalization or Imagining Utopia?
Authors: Ray Larry
Published: August 2009

This article reviews the implications of the collapse of Communism in Europe for some themes in recent social theory. It was often assumed that 1989 was part of a global process of normalization and routinization of social life that had been left behind earlier utopian hopes. Nothing that utopia is open to various interpretations, including utopias of the everyday, this article suggests, first that there were utopian dimensions to 1989, and, second, that these hopes continue to influence contemporary social and political developments. The continuing role of substantive utopian expectations is illustrated with reference to the politics of lustration in Poland and the rise of nationalist parties in Hungary. This analysis is placed in the context of the already apparent impact of the global economic crisis in post-communist countries. It concludes that the unevenness and diversity of the post-1989 world elude overly generalized attempts at theorization and demand more nuanced analyses.
Transnational Culture and the Political Transformation of East-Central Europe
Authors: Brier Robert
Published: August 2009

In social scientific studies of Europe’s new democracies, there has emerged an analytical approach which transcends the teleology of ‘transitology’ and, focusing on the impact of culture and history, is sensitive to the contingencies and ‘eventfulness’ of social transformations. The main thrust of this article is that such a culturo-historical approach may prove useful not only in assessing the different results to which the processes of democratization lead at the national level, but also to assess the general direction of political change after 1989 towards democracy. Building on Eisenstadt’s notion of modernity as a cultural and political program, this article therefore attempts to understand the revolutions of 1989 not only as the mere sum of particular national events, but also as part of an ‘entangled history’, that is, as a common, transnational phenomenon which was based on and articulated a shared cultural understanding.
Europeanization, Religion and Collective Identities in an Enlarging Europe
Authors: Spohn Willfried
Published: August 2009

This article analyzes the conflictive role of religion in post-1989 Europe. Three major reasons for this are addressed: first, the restoration of structural and cultural pluralism of European civilization since the breakdown of communism entails the reconstitution of the full diversity of European religion. Second, international migration as a crucial part of globalization has intensified, contributing to the transformation of Europe into a complex of multi-cultural and pluri-religious societies. Third, the wave of contemporary globalization has been accompanied by an intensification of inter-civilizational and inter-religious encounters and conflicts — particularly between Christianity and Islam. As a result, European integration and enlargement as a secular and humanist mode of cultural integration and religious governance are basically challenged by this three-fold revitalization of religion. The growing tendency is to respond to this challenge by enhancing the Christian foundations of Europe rather than, as this article argues, to follow a more cosmopolitan, secularist and religious pluralist mode of European cultural integration.
Agreeing to Disagree on the Legacies of Recent History
Authors: Kattago Siobhan
Published: August 2009

Since 1989, social change in Europe has moved between two stories. The first being a politics of memory emphasizing the specificity of culture in national narratives, and the other extolling the virtues of the Enlightenment heritage of reason and humanity. While the Holocaust forms a central part of West European collective memory, national victimhood of former Communist countries tends to occlude the centrality of the Holocaust. Highlighting examples from the Estonian experience, this article asks whether attempts to find one single European memory of trauma ignore the complexity of history and are thus potentially disrespectful to those who suffered under both Communism and National Socialism. Pluralism in the sense of Hannah Arendt and Isaiah Berlin is presented as a way in which to move beyond the settling of scores in the past and towards a respectful recognition and acknowledgement of historical difference.
Gusts of Change
Authors: Roudometof Victor
Published: August 2009

Since the 1960s, the concepts of the ‘global’ and the ‘transnational’ have challenged the state-centred orientation of several disciplines. By 1989, the ‘global’ contained sufficient ambiguity and conceptual promise to emerge as a potentially new central concept to replace the conventional notion of modernity. The consequences of the 1989 revolutions for this emerging concept were extensive. As a result of the post-communist ‘New World Order’, a new vision of a single triumphant political and economic system was put forward. With the ‘globalizing of modernity’ as a description of the post-1989 reality, ‘globalization’ became the policy mantra of the Clinton and Blair administrations up until the late 1990s when ‘anti-globalization’ activists were able to question the salience of this dominant theory of ‘globalization’. In scholarly discussion, ‘globalization’ became a floating signifier to be filled with a variety of disciplinary and political meanings. In the post-9/11 era, this Western-centred ‘globalization’ has been conceptually linked to cosmopolitanism while it has played a minor role in the multiple modernities agenda. The article concludes with an assessment of the current status of the ‘global’ in theory and research.
1989, Contested Memories and the Shifting Cognitive Maps of Europe
Authors: Challand Benoît
Published: August 2009

Addressing attempts to define a common European memory on the theme of the Holocaust, and transformations of the Cold War discourses on totalitarianism and democracy. The article conceptualizes the persistent forms and new constellations of alterity that reproduce an East—West divide. The article shows that cognitive debates about Europe hint at constantly shifting relations between various parts of Europe and between Europe and its neighbors. A relational conceptual vocabulary is proposed to describe the debates on Europe following 1989. Cleavages and social distancing can be expressed in terms of different temporal locations (allochronism) which, when merged with a normative stance, can lead to a situation of heterochrony.
Constructing crime, framing disaster
Authors: Berger Dan
Published: August 2009

This article argues that the media frames utilized in the first month after Hurricane Katrina legitimated punishment as disaster policy through lurid reports of individual crime. The application of prevailing state policies led to a quick embrace of punitive policing and incarceration, and journalistic routines ended up supporting this process. Although journalists openly expressed their disgust with state neglect, news conventions nonetheless criminalized much of the New Orleans population and suggested militarized policing and imprisonment as fundamental to restore order. Lacking credible sources, reporters relied on rumors and helped create a racialized ‘looter class’ that aided state efforts to regain control through existing policies of mass incarceration rather than mutual aid or state welfare. Even though various media outlets recanted the more extreme elements of this coverage, the tropes they employed created a lasting effect. Building off Stuart Hall et al.’s (1978) analysis of a moral panic over mugging in 1970s England, this article examines both the conventions and consequences of this crisis coverage. The result, I argue, bolstered the existing crisis of incarceration.
The political economy of risk and the new governance of youth crime
Authors: Gray Patricia
Published: August 2009

Despite heated debate over levels of convergence and diversity, risk assessment and behaviour management have become central themes of international youth justice strategies in several jurisdictions. However such strategies represent more than a new formula for the governance of delinquent youth as they also offer a novel framework for articulating class discipline within the context of a reconfigured style of socio-political and economic leadership. Postmodern social theory has rendered old-fashioned any attempts to meta-theorize about class in criminological research and it is therefore not intended to rekindle the grand narratives generated form orthodox Marxism. Nevertheless, class inequalities, along with those generated by other social forces, continue to structure many social relations and this article will explore the complex micro-processes by which class interests are articulated in youth justice relations through the twin processes of the individualization of risk and the responsibilization of young offenders. The argument is illustrated through research on the outcomes of government sponsored education, training and employment programmes for young offenders in England and Wales.
What Does Race Have to Do with Ugly Betty?
Authors: Esposito Jennifer
Published: August 2009

This article examines ABC’s television comedy Ugly Betty, in particular one episode that explores race-based affirmative action decisions and quotas, to argue that race and racial categories are ever more present in our society and that they need to be. Asserting how and in what ways race “matters” is important in a social and political climate that often suggests race dare not speak its name. Circulating within sociology and education discourse is the notion of a “color-blind society” (meaning that we no longer see color or that the color of one’s skin will not determine his or her life chances). This idea has been has been recently redefined by the media as “postracial” (meaning that we have moved beyond race and that race no longer structures our thinking or our actions). Either discourse silences talk of racial privilege and disadvantage. As a discursive racial project, the Ugly Betty text helps reify notions of race and difference.
Paying for Fewer Imports
Authors: Weissmann Elke
Published: August 2009

This article investigates the impact of the BBC license fee negotiations between 1975 and 1981 on the relationship between the broadcaster and American programming. It highlights the increasing dependence of the BBC on imported programming and on the export of content to America due to a series of below-inflation increases that left the BBC financially weakened. The article’s main concern, however, is with the management of attitudes toward American television that were exploited by key executive personnel in the United Kingdom for political ends: the campaign for a higher license fee. The author argues that the successive campaigns during the late 1970s exploited fears of Americanization to emphasize the lowering of quality in the output on the BBC, which connected to debates around public service ideals and hence, to the legitimization of the license fee. The campaigns ultimately also affected public attitudes, which increasingly registered American programming in relation to the lowering of standards on the BBC.
Civic Talk and Civic Participation
Authors: Klofstad Casey A.
Published: August 2009

Individuals who discuss politics and current events in their social network also participate in civic activities. However, analytical biases make it difficult to show a causal relationship between these two phenomena. To obtain a more accurate measurement of the effect that civic talk has on civic participation, data were collected through a panel study conducted on students who were randomly assigned to their college dormitory roommates. These data show that engaging in civic talk causes civic participation. The evidence also shows that the civic talk effect is mitigated for individuals who are not already predisposed to participate in civic activities.
Social Networks and American Politics
Authors: Heaney Michael T. , McClurg Scott D.
Published: August 2009

This article overviews the special issue on “Social Networks and American Politics.” The authors explain that social network analysis is a multimethod set of approaches to examining the pattern of connections that are created among individuals and institutions when they engage in their daily activities. It is especially valuable when research problems are about (a) the flow of information; (b) coordination, cooperation, or trust; (c) informal organization; or (d) multiple levels of organization. In addressing these problems, network analysis has expanded during the last decade within the study of American politics, contributing to knowledge about political institutions, behavior, and network theory. Promising directions for future research include the study of power, preference aggregation, information flow and transaction costs, and network dynamics.
How Groups Learn:The Role of Communication Patterns, Cue Recognition, Context Facility, and Cultural Intelligence
Authors: Silberstang Joyce , London Manuel
Published: August 2009

This article explores the role of group learning by focusing on how intragroup communication patterns (implicit and explicit) influence learning readiness dimensions (cue recognition, context facility, and cultural intelligence), which in turn influences the group’s ability to learn and the type of leaning that occurs. Groups with high levels of cue recognition, context facility, and cultural intelligence are more likely to discuss difficult issues and decide whether and how much the group needs to learn than groups with low levels. Communication patterns and learning readiness contribute to adaptive, generative, and transformative learning, fostering the type of learning that is most beneficial for the group at the time. Human resource development professionals can help groups improve their communication patterns and thus strengthen learning readiness and enhance their ability to effectively communicate, learn, and reach their goals.
Caring as a Managerial Strategy
Authors: Kroth Michael , Keeler Carolyn
Published: August 2009

The purpose of this article is to broaden the discourse about caring as a managerial strategy by describing caring from three perspectives: nursing, education, and management. The authors suggest that current organizational models inadequately address the caring connection between manager and employee. Definitions of managerial caring and care building are offered. A model, the Recursive Model of Manager—Employee Caring, is proposed and discussed. Potential lines of inquiry associated with the model and implications for HRD are reviewed.
Human Nature, Organizational Politics, and Human Resource Development
Authors: Vredenburgh Donald , Shea-VanFossen Rita
Published: August 2009

The human species possesses psychological attributes derived from evolutionary natural selection. This human nature has developed historically in adaptation to fundamental conditions that continue to exist in modern work organizations. Thus genetic predispositions can cause contemporary self-interested behavior amid organizational threats and opportunities. Select individual attributes and particular organizational conditions suggest specific political behaviors, represented here as theoretical propositions about organizational politics.The prevalence of organizational politics defines a need for human resource development programs to lessen dysfunctional politics. Organizations can act through individual and organizational development programs based on needs assessments to increase awareness of dysfunctional outcomes, discourage political behaviors, and encourage alternative practices. In addition, the human resources function can contribute organizationally by fostering positive organizational politics in the form of responsive interpersonal leadership.
Optimisation of brass plating condition in plating of Patented steel wire
Authors: Saadatmand M , Sadeghpour S , Aghazadeh Mohandesi J
Published: August 2009

The patented steel wire that is exclusively used for reinforcement of rubber products such as tyres and hoses is usually provided with a coating of brass alloy. Brass plating increases the fracture life during the drawing operation and also improves adhesion to rubber. In order to obtain suitable solution for brass plating with more efficiency and environmentally safer condition, this paper deals with the effect of free cyanide in plating solution, electroplating time and voltage on the quality and thickness of coating. The most suitable concentration of free cyanide was found as 5 g L−1. The ideal thickness of coating with maximum drawability is 2–3 μm and the optimum conditions for brass plating of wire has been achieved under voltage of 1·5–2 V, pH = 11 for 2–7 min, and temperature of 30°C. Also the mean adhesion strength between rubber and patented wire under optimum condition is ∼7·3 MPa.
Alpha adrenergic modulation on effects of norepinephrine transporter inhibitor reboxetine in five-choice serial reaction time task
Authors: Liu Yia-Ping , Lin Yu-Lung , Chuang Chia-Hsin , Kao Yu-Cheng , Chang Shang-Tang , Tung Che-Se
Published: August 2009

The study examined the effects of a norepinephrine transporter (NET) inhibitor reboxetine (RBX) on an attentional performance test. Adult SD rats trained with five-choice serial reaction time task (5-CSRTT) were administered with RBX (0, 3.0 and 10 mg/kg) in the testing day. Alpha-1 adrenergic receptor antagonist PRA and alpha-2 adrenergic receptor antagonist RX821002 were used to clarify the RBX effect. Results revealed that rat received RBX at 10 mg/kg had an increase in the percentage of the correct response and decreases in the numbers of premature response. Alpha-1 adrenergic receptor antagonist Prazosin (PRA) at 0.1 mg/kg reversed the RBX augmented correct responding rate. However, alpha-2 adrenergic receptor antagonist RX821002 at 0.05 and 0.1 mg/kg dose dependently reversed the RBX reduced impulsive responding. Our results suggested that RBX as a norepinephrine transporter inhibitor can be beneficial in both attentional accuracy and response control and alpha-1 and alpha-2 adrenergic receptors might be involved differently.
CAPIH: A Web interface for comparative analyses and visualization of host-HIV protein-protein interactions
Authors: Lin Fan-Kai , Pan Chia-Lin , Yang Jinn-Moon , Chuang Trees-Juen , Chen Feng-Chi
Published: August 2009

Background

The Human Immunodeficiency Virus type one (HIV-1) is the major causing pathogen of the Acquired Immune Deficiency Syndrome (AIDS). A large number of HIV-1-related studies are based on three non-human model animals: chimpanzee, rhesus macaque, and mouse. However, the differences in host-HIV-1 interactions between human and these model organisms have remained unexplored.

Description

Here we present CAPIH (C omparative A nalysis of P rotein I nteractions for H IV-1), the first web-based interface to provide comparative information between human and the three model organisms in the context of host-HIV-1 protein interactions. CAPIH identifies genetic changes that occur in HIV-1-interacting host proteins. In a total of 1,370 orthologous protein sets, CAPIH identifies ~86,000 amino acid substitutions, ~21,000 insertions/deletions, and ~33,000 potential post-translational modifications that occur only in one of the four compared species. CAPIH also provides an interactive interface to display the host-HIV-1 protein interaction networks, the presence/absence of orthologous proteins in the model organisms in the networks, the genetic changes that occur in the protein nodes, and the functional domains and potential protein interaction hot sites that may be affected by the genetic changes. The CAPIH interface is freely accessible at http://bioinfo-dbb.nhri.org.tw/capih.

Conclusion

CAPIH exemplifies that large divergences exist in disease-associated proteins between human and the model animals. Since all of the newly developed medications must be tested in model animals before entering clinical trials, it is advisable that comparative analyses be performed to ensure proper translations of animal-based studies. In the case of AIDS, the host-HIV-1 protein interactions apparently have differed to a great extent among the compared species. An integrated protein network comparison among the four species will probably shed new lights on AIDS studies.
Presence of activating KRAS mutations correlates significantly with expression of tumour suppressor genes DCN and TPM1 in colorectal cancer
Authors: Mlakar Vid , Berginc Gašper , Volavšek Metka , Štor Zdravko , Rems Miran , Glavač Damjan
Published: August 2009

Background

Despite identification of the major genes and pathways involved in the development of colorectal cancer (CRC), it has become obvious that several steps in these pathways might be bypassed by other as yet unknown genetic events that lead towards CRC. Therefore we wanted to improve our understanding of the genetic mechanisms of CRC development.

Methods

We used microarrays to identify novel genes involved in the development of CRC. Real time PCR was used for mRNA expression as well as to search for chromosomal abnormalities within candidate genes. The correlation between the expression obtained by real time PCR and the presence of the KRAS mutation was investigated.

Results

We detected significant previously undescribed underexpression in CRC for genes SLC26A3, TPM1 and DCN, with a suggested tumour suppressor role. We also describe the correlation between TPM1 and DCN expression and the presence of KRAS mutations in CRC. When searching for chromosomal abnormalities, we found deletion of the TPM1 gene in one case of CRC, but no deletions of DCN and SLC26A3 were found.

Conclusion

Our study provides further evidence of decreased mRNA expression of three important tumour suppressor genes in cases of CRC, thus implicating them in the development of this type of cancer. Moreover, we found underexpression of the TPM1 gene in a case of CRCs without KRAS mutations, showing that TPM1 might serve as an alternative path of development of CRC. This downregulation could in some cases be mediated by deletion of the TPM1 gene. On the other hand, the correlation of DCN underexpression with the presence of KRAS mutations suggests that DCN expression is affected by the presence of activating KRAS mutations, lowering the amount of the important tumour suppressor protein decorin.
Acupuncture in acute herpes zoster pain therapy (ACUZoster) – design and protocol of a randomised controlled trial
Authors: Fleckenstein Johannes , Kramer Sybille , Hoffrogge Philipp , Thoma Sarah , Lang Philip M , Lehmeyer Lukas , Schober Gabriel M , Pfab Florian , Ring Johannes , Weisenseel Peter , Schotten Klaus J , Mansmann Ulrich , Irnich Dominik
Published: August 2009

Background

Acute herpes zoster is a prevalent condition. One of its major symptoms is pain, which can highly influence patient's quality of life. Pain therapy is limited. Acupuncture is supposed to soften neuropathic pain conditions and might therefore act as a therapeutic alternative. Objective of the present study is to investigate whether a 4 week semi-standardised acupuncture is non-inferior to sham laser acupuncture and the anticonvulsive drug gabapentine in the treatment of pain associated with herpes zoster.

Methods/Design

Three-armed, randomised, placebo-controlled trial with a total follow-up time of 6 months. Up to estimated 336 patients (interim analyses) with acute herpes zoster pain (VAS > 30 mm) will be randomised to one of three groups (a) semi-standardised acupuncture (168 patients); (b) gabapentine with individualised dosage between 900–3600 mg/d (84 patients); (c) sham laser acupuncture. Intervention takes place over 4 weeks, all patients will receive analgesic therapy (non-opioid analgesics: metamizol or paracetamol and opioids: tramadol or morphine). Therapy phase includes 4 weeks in which group (a) and (c) consist of 12 sessions per patient, (b) visits depend on patients needs. Main outcome measure is to assess the alteration of pain intensity before and 1 week after treatment sessions (visual analogue scale VAS 0–100 mm). Secondary outcome measure are: alteration of pain intensity and frequency of pain attacks; alteration of different aspects of pain evaluated by standardised pain questionnaires (NPI, PDI, SES); effects on quality of life (SF 36); analgesic demand; alteration of sensoric perception by systematic quantitative sensory testing (QST); incidence of postherpetic neuralgia; side effects and cost effectiveness. Credibility of treatments will be assessed.

Discussion

This study is the first large-scale randomised placebo controlled trial to evaluate the efficacy of acupuncture compared to gabapentine and sham treatment and will provide valuable new information about the clinical and physiological effects of acupuncture and gabapentine in the treatment of acute herpes zoster pain. The study has been pragmatically designed to ensure that the study findings can be implemented into clinical practice if acupuncture can be shown to be an effective treatment strategy in acute herpes zoster pain.

Trial registration

NCT00885586
Exogenous Cx43 expression decrease cell proliferation rate in rat hepatocarcinoma cells independently of functional gap junction
Authors: Ionta Marisa , Ferreira Raphael Adolpho Sant'ana , Pfister Sandra Cristina , Machado-Santelli Gláucia Maria
Published: August 2009

Background

Gap junction intercellular communication (GJIC) is considered to play a role in the regulation of homeostasis because it regulates important processes, such as cell proliferation and cell differentiation. A reduced or lost GJIC capacity has been observed in solid tumors and studies have demonstrated that GJIC restoration in tumor cells contribute to reversion of the transformed phenotype. This observation supports the idea that restoration of the functional channel is essential in this process. However, in the last years, reports have proposed that just the increase in the expression of specific connexins can contribute to reversion of the malign phenotype in some tumor cells. In the present work, we studied the effects of exogenous Connexin 43 (Cx43) expression on the proliferative behavior and phenotype of rat hepatocarcinoma cells.

Results

The exogenous Cx43 did not increase GJIC capacity of transfected cells, but it was critical to decrease the cell proliferation rate as well as reorganization of the actin filaments and cell flattening. We also observed more adhesion capacity to substrate after Cx43 transfection.

Conclusion

Cx43 expression leads to a decrease of the growth of the rat hepatocellular carcinoma cells and it contributes to the reversion of the transformed phenotype. These effects were independent of the GJIC and were probably associated with the phosphorylation pattern changes and redistribution of the Cx43 protein.
The utilization of humanized mouse models for the study of human retroviral infections
Authors: Van Duyne Rachel , Pedati Caitlin , Guendel Irene , Carpio Lawrence , Kehn-Hall Kylene , Saifuddin Mohammed , Kashanchi Fatah
Published: August 2009

The development of novel techniques and systems to study human infectious diseases in both anin vitroandin vivosettings is always in high demand. Ideally, small animal models are the most efficient method of studying human afflictions. This is especially evident in the study of the human retroviruses, HIV-1 and HTLV-1, in that current simian animal models, though robust, are often expensive and difficult to maintain. Over the past two decades, the construction of humanized animal models through the transplantation and engraftment of human tissues or progenitor cells into immunocompromised mouse strains has allowed for the development of a reconstituted human tissue scaffold in a small animal system. The utilization of small animal models for retroviral studies required expansion of the early CB-17scid/scidmouse resulting in animals demonstrating improved engraftment efficiency and infectivity. The implantation of uneducated human immune cells and associated tissue provided the basis for the SCID-hu Thy/Liv and hu-PBL-SCID models. Engraftment efficiency of these tissues was further improved through the integration of the non-obese diabetic (NOD) mutation leading to the creation of NODSCID, NOD/Shi-scid IL2rγ-/-, and NOD/SCID β2-microglobulinnullanimals. Further efforts at minimizing the response of the innate murine immune system produced the Rag2-/-γc-/-model which marked an important advancement in the use of human CD34+ hematopoietic stem cells. Together, these animal models have revolutionized the investigation of retroviral infectionsin vivo.
Partial direct contact transmission in ferrets of a mallard H7N3 influenza virus with typical avian-like receptor specificity
Authors: Song Haichen , Wan Hongquan , Araya Yonas , Perez Daniel R
Published: August 2009

Background

Avian influenza viruses of the H7 subtype have caused multiple outbreaks in domestic poultry and represent a significant threat to public health due to their propensity to occasionally transmit directly from birds to humans. In order to better understand the cross species transmission potential of H7 viruses in nature, we performed biological and molecular characterizations of an H7N3 virus isolated from mallards in Canada in 2001.

Results

Sequence analysis that the HA gene of the mallard H7N3 virus shares 97% identity with the highly pathogenic avian influenza (HPAI) H7N3 virus isolated from a human case in British Columbia, Canada in 2004. The mallard H7N3 virus was able to replicate in quail and chickens, and transmitted efficiently in quail but not in chickens. Interestingly, although this virus showed preferential binding to analogs of avian-like receptors with sialic acid (SA) linked to galactose in an α2–3 linkage (SAα2–3Gal), it replicated to high titers in cultures of primary human airway epithelial (HAE) cells, comparable to an avian H9N2 influenza virus with human-like α2–6 linkage receptors (SAα2–6Gal). In addition, the virus replicated in mice and ferrets without prior adaptation and was able to transmit partially among ferrets.

Conclusion

Our findings highlight the importance and need for systematic in vitro and in vivo analysis of avian influenza viruses isolated from the natural reservoir in order to define their zoonotic potential.
Increased Chemokine Signaling in a Model of HIV1-Associated Peripheral Neuropathy
Authors: Bhangoo Sonia K , Ripsch Matthew S , Buchanan David J , Miller Richard J , White Fletcher A
Published: August 2009

Painful distal sensory polyneuropathy (DSP) is the most common neurological complication of HIV1 infection. Although infection with the virus itself is associated with an incidence of DSP, patients are more likely to become symptomatic following initiation of nucleoside reverse transcriptase inhibitor (NRTI) treatment. The chemokines monocyte chemoattractant protein-1 (MCP1/CCL2) and stromal derived factor-1 (SDF1/CXCL12) and their respective receptors, CCR2 and CXCR4, have been implicated in HIV1 related neuropathic pain mechanisms including NRTI treatment in rodents. Utilizing a rodent model that incorporates the viral coat protein, gp120, and the NRTI, 2'3'-dideoxycytidine (ddC), we examined the degree to which chemokine receptor signaling via CCR2 and CXCR4 potentially influences the resultant chronic hypernociceptive behavior. We observed that following unilateral gp120 sciatic nerve administration, rats developed profound tactile hypernociception in the hindpaw ipsilateral to gp120 treatment. Behavioral changes were also present in the hindpaw contralateral to the injury, albeit delayed and less robust. Using immunohistochemical studies, we demonstrated that MCP1 and CCR2 were upregulated by primary sensory neurons in lumbar ganglia by post-operative day (POD) 14. The functional nature of these observations was confirmed using calcium imaging in acutely dissociated lumbar dorsal root ganglion (DRG) derived from gp120 injured rats at POD 14. Tactile hypernociception in gp120 treated animals was reversed following treatment with a CCR2 receptor antagonist at POD 14. Some groups of animals were subjected to gp120 sciatic nerve injury in combination with an injection of ddC at POD 14. This injury paradigm produced pronounced bilateral tactile hypernociception from POD 14–48. More importantly, functional MCP1/CCR2 and SDF1/CXCR4 signaling was present in sensory neurons. In contrast to gp120 treatment alone, the hypernociceptive behavior associated with the injury plus drug combination was only effectively reversed using the CXCR4 antagonist AMD3100. These studies indicate that the functional upregulation of CCR2 and CXCR4 signaling systems following a combination of gp120 and an NRTI are likely to be of central importance to associated DSP and may serve as potential therapeutic targets for treatment of this syndrome.
Efficacy of prescribed injectable diacetylmorphine in the Andalusian trial: Bayesian analysis of responders and non-responders according to a multi domain outcome index
Authors: Perea-Milla Emilio , Ayçaguer Luis Carlos Silva , Cerdà Joan Carles March , Saiz Francisco González , Rivas-Ruiz Francisco , Danet Alina , Vallecillo Manuel Romero , Oviedo-Joekes Eugenia
Published: August 2009

Background

The objective of this research was to evaluate data from a randomized clinical trial that tested injectable diacetylmorphine (DAM) and oral methadone (MMT) for substitution treatment, using a multi-domain dichotomous index, with a Bayesian approach.

Methods

Sixty two long-term, socially-excluded heroin injectors, not benefiting from available treatments were randomized to receive either DAM or MMT for 9 months in Granada, Spain. Completers were 44 and data at the end of the study period was obtained for 50. Participants were determined to be responders or non responders using a multi-domain outcome index accounting for their physical and mental health and psychosocial integration, used in a previous trial. Data was analyzed with Bayesian methods, using information from a similar study conducted in The Netherlands to select a priori distributions. On adding the data from the present study to update the a priori information, the distribution of the difference in response rates were obtained and used to build credibility intervals and relevant probability computations.

Results

In the experimental group (n = 27), the rate of responders to treatment was 70.4% (95% CI 53.2-87.6), and in the control group (n = 23), it was 34.8% (95% CI 15.3-54.3). The probability of success in the experimental group using the a posteriori distributions was higher after a proper sensitivity analysis. Almost the whole distribution of the rates difference (the one for diacetylmorphine minus methadone) was located to the right of the zero, indicating the superiority of the experimental treatment.

Conclusion

The present analysis suggests a clinical superiority of injectable diacetylmorphine compared to oral methadone in the treatment of severely affected heroin injectors not benefiting sufficiently from the available treatments.

Trial Registration

Current Controlled Trials ISRCTN52023186
The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue
Authors: Abreu Joana RF , de Launay Daphne , Sanders Marjolein E , Grabiec Aleksander M , Sande Marleen G van de , Tak Paul P , Reedquist Kris A
Published: August 2009

Introduction

Fibroblast-like synoviocytes (FLS) from rheumatoid arthritis (RA) patients share many similarities with transformed cancer cells, including spontaneous production of matrix metalloproteinases (MMPs). Altered or chronic activation of proto-oncogenic Ras family GTPases is thought to contribute to inflammation and joint destruction in RA, and abrogation of Ras family signaling is therapeutic in animal models of RA. Recently, expression and post-translational modification of Ras guanine nucleotide releasing factor 1 (RasGRF1) was found to contribute to spontaneous MMP production in melanoma cancer cells. Here, we examine the potential relationship between RasGRF1 expression and MMP production in RA, reactive arthritis, and inflammatory osteoarthritis synovial tissue and FLS.

Methods

Expression of RasGRF1, MMP-1, MMP-3, and IL-6 was detected in synovial tissue by immunohistochemistry and stained sections were evaluated by digital image analysis. Expression of RasGRF1 in FLS and synovial tissue was also assessed by immunoblotting. Double staining was performed to detect proteins in specific cell populations, and cells producing MMP-1 and MMP-3. RasGRF1 expression was manipulated in RA FLS by cDNA transfection and gene silencing, and effects on MMP-1, TIMP-1, MMP-3, IL-6, and IL-8 production measured by ELISA.

Results

Expression of RasGRF1 was significantly enhanced in RA synovial tissue, and detected in FLS and synovial macrophages in situ. In cultured FLS and synovial biopsies, RasGRF1 was detected by immunoblotting as a truncated fragment lacking its negative regulatory domain. Production of MMP-1 and MMP-3 in RA but not non-RA synovial tissue positively correlated with expression of RasGRF1 and co-localized in cells expressing RasGRF1. RasGRF1 overexpression in FLS induced production of MMP-3, and RasGRF1 silencing inhibited spontaneous MMP-3 production.

Conclusions

Enhanced expression and post-translational modification of RasGRF1 contributes to MMP-3 production in RA synovial tissue and the semi-transformed phenotype of RA FLS.
Improved base calling for the Illumina Genome Analyzer using machine learning strategies
Authors: Kircher Martin , Stenzel Udo , Kelso Janet
Published: August 2009

The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.
MouseCyc: a curated biochemical pathways database for the laboratory mouse
Authors: Evsikov Alexei V , Dolan Mary E , Genrich Michael P , Patek Emily , Bult Carol J
Published: August 2009

Linking biochemical genetic data to the reference genome for the laboratory mouse is important for comparative physiology and for developing mouse models of human biology and disease. We describe here a new database of curated metabolic pathways for the laboratory mouse called MouseCyc http://mousecyc.jax.org. MouseCyc has been integrated with genetic and genomic data for the laboratory mouse available from the Mouse Genome Informatics database and with pathway data from other organisms, including human.
Enhancer-promoter communication at theDrosophila engrailedlocus
Authors: Kwon Deborah , Mucci Diane , Langlais Kristofor K. , Americo Jeffrey L. , DeVido Sarah K. , Cheng Yuzhong , Kassis Judith A.
Published: August 2009

Enhancers are often located many tens of kilobases away from the promoter they regulate, sometimes residing closer to the promoter of a neighboring gene. How do they know which gene to activate? We have used homing P[en]constructs to study the enhancer-promoter communication at the engrailed locus. Here we show that engrailed enhancers can act over large distances, even skipping over other transcription units,choosing the engrailed promoter over those of neighboring genes. This specificity is achieved in at least three ways. First, early acting engrailed stripe enhancers exhibit promoter specificity. Second, a proximal promoter-tethering element is required for the action of the imaginal disc enhancer(s). Our data suggest that there are two partially redundant promoter-tethering elements. Third, the long-distance action of engrailed enhancers requires a combination of the engrailedpromoter and sequences within or closely linked to the promoter proximal Polycomb-group response elements. These data show that multiple mechanisms ensure proper enhancer-promoter communication at the Drosophila engrailed locus.
Kinematics, hydrodynamics and force production of pleopods suggest jet-assisted walking in the American lobster (Homarus americanus)
Authors: Lim Jeanette L. , DeMont M. Edwin
Published: August 2009

The American lobster (Homarus americanus) displays a diverse set of locomotory behaviours that includes tail flips, walking and paddling. Paddling is carried out by the four pairs of paddle-shaped pleopods on the ventral abdomen. Although it is recognized that pleopod-generated fluid flows have some locomotory role in adults, reports on their relative importance in locomotion are inconsistent. This paper integrates experimental kinematics and hydrodynamics of lobster pleopod beating to determine the mechanism and magnitude of pleopod force production. A kinematic analysis of pleopod beating in live lobsters showed that the pleopods execute an adlocomotory metachronal beating pattern. We modelled in vivo pleopod kinematics with a set of simple trigonometric functions, and used these functions to program a mechanical lobster model consisting of motor-driven pleopods on a lobster abdomen exoskeleton. Based on flow visualizations obtained from applying particle image velocimetry to the lobster model, we propose that the unsteady metachronal kinematics of the pleopods can maximize thrust by exploiting forces arising from individual pleopod activity and interactions among adjacent pairs. The pleopods continuously entrain fluid surrounding the lobster and create a caudally directed fluid jet oriented parallel to the substratum. Inputting wake morphology and velocity data into a simplified model for steady jet thrust showed that the pleopods of the lobster model produced 27–54 mN of thrust, which is comparable to the propulsive forces generated by other proficient swimmers. These results suggest that lobster pleopods are capable of producing forces of a magnitude that could assist the walking legs in forward propulsion.
Sugar feedingviatrehalose haemolymph concentration affects sex pheromone production in matedHeliothis virescensmoths
Authors: Foster Stephen
Published: August 2009

Long-distance, female-produced sex pheromones are widespread among moths. Larval feeding provides most of the nutrients for development of these insects but is not thought to influence the de novo production of the fatty-acid derived compounds used as pheromones by most species. Feeding on plant nectar (sugar) by adult moths is important for increasing female fitness and also for the pollination of many plant species. In this paper, I show that feeding on sucrose solution, as opposed to water, increases sex pheromone titre in mated, but not virgin, female Heliothis virescens. Mating caused a rapid decrease in haemolymph trehalose concentration, which was restored to near-virgin levels by sugar ingestion. When isolated mated female abdomens were cultured with different concentrations of trehalose, pheromone titre increased with increasing trehalose concentration. This effect was not observed when abdomens were cultured on saline containing the sugar rhamnose,which insects cannot metabolise to glucose. Virgins injected with the juvenile hormone (JH) analogue, methoprene, showed the same effects as mated females with respect to pheromone titre and haemolymph trehalose concentration. Thus,following mating increases in JH titre increase demand for, and lowering of,blood sugar to develop oocytes, which can be compensated for by sugar ingestion. Haemolymph trehalose concentration probably influences glycolysis in gland cells and, consequently, levels of cytosolic citrate and acetyl-CoA for pheromone biosynthesis. This increase in pheromone titre in sugar-fed,mated females may facilitate further mating and increased fecundity. Thus,exogenous sugar feeding is behaviourally and physiologically integrated with endogenous JH titre to maximise female fitness.
Changes in thyroid hormone reception precede SWS1 opsin downregulation in trout retina
Authors: Raine J. C. , Hawryshyn C. W.
Published: August 2009

Rainbow trout undergo natural cone degeneration and thus are interesting models for examining mechanisms of neural degeneration. They have ultraviolet-sensitive (UVS) cones that are lost over most of the retina during development; only a small functional population remains in the dorsal retina. How this spatial distribution of UVS cones is maintained is unclear. Thyroxine(T4) induces UVS cone loss, and local thyroid hormone regulation was hypothesized to control UVS cone distribution. Thyroid hormone receptor alpha(TRα), thyroid hormone receptor beta (TRβ) and Type 2 deiodinase(D2) regulate thyroid hormone exposure to target cells. Regional retinal expression of these genes was investigated during exogenous T4 treatment and natural smoltification of rainbow trout. Each retina from dark-adapted parr,T4-treated parr and natural smolts was divided into four quadrants, and total RNA was isolated. Quantitative real-time RT-PCR analysis demonstrated that all retinal quadrants had increased accumulation of TRβ transcripts 2 days post-T4 treatment, corresponding to initiation of SWS1 opsin downregulation. Smolts exhibited decreased accumulation of TRαand TRβ transcripts in all quadrants, but this effect was most pronounced in the dorso-temporal (DT) retinal quadrant where UVS cones persist. By contrast, in 2 day T4-treated parr, the DT quadrant showed increased expression of TRα and TRβ. Furthermore, D2 transcripts decreased in the DT quadrant of T4-treated parr but increased in the DT quadrant of smolts. These results suggest that T4 upregulates TRβ expression to initiate SWS1 opsin downregulation, while TRα and TRβ downregulation occurs to prevent natural loss of UVS cones from the DT retina.