bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolic Dysfunction-Associated Steatotic Liver Disease and Alcohol-Associated Liver Disease: Liver DNA Methylation Analysis—A Systematic Review
Authors: Stols-Gonçalves Daniela , Meijnikman Abraham S. , Tristão Luca Schiliró , Santos Clara Lucato dos , Denswil Nerissa P. , Verheij Joanne , Bernardo Wanderley M. , Nieuwdorp Max
Published: November 2024

Background: Metabolic dysfunction-associated liver disease (MASLD) and alcohol-associated liver disease (ALD) are among the leading causes of liver disease worldwide. The exact roles of epigenetic factors in both diseases remains largely unknown. In this context, liver DNA methylation remains a field that requires further exploration and understanding. Methods: We performed a systematic review of liver DNA methylation in humans with MASLD or ALD using Ovid MEDLINE, Ovid Embase, and Cochrane Library. We included human studies where liver DNA methylation was assessed in patients with MASLD and/or ALD. The Rayyan platform was used to select studies. Risk of bias was assessed with the “risk of bias in non-randomized studies of interventions” tool, ROBINS-I. We performed pathway analysis using the most important differentially methylated genes selected in each article. Results: Fifteen articles were included in this systematic review. The risk of bias was moderate to serious in all articles and bias due to confounding and patient selection was high. Sixteen common pathways, containing differentially methylated genes, including cancer pathways, were identified in both diseases. Conclusions: There are common pathways, containing differentially methylated genes, in ALD and MASLD, such as pathways in cancer and peroxisome proliferator-activated receptor (PPAR) signaling pathways. In MASLD, the insulin signaling pathway is one of the most important, and in ALD, the MAPK signaling pathway is the most important. Our study adds one more piece to the puzzle of the mechanisms involved in steatotic liver disease.
Loop Extrusion Machinery Impairments in Models and Disease
Authors: Ryzhkova Anastasiya , Maltseva Ekaterina , Battulin Nariman , Kabirova Evelyn
Published: November 2024

Structural maintenance of chromosomes (SMC) complexes play a crucial role in organizing the three-dimensional structure of chromatin, facilitating key processes such as gene regulation, DNA repair, and chromosome segregation. This review explores the molecular mechanisms and biological significance of SMC-mediated loop extrusion complexes, including cohesin, condensins, and SMC5/6, focusing on their structure, their dynamic function during the cell cycle, and their impact on chromatin architecture. We discuss the implications of impairments in loop extrusion machinery as observed in experimental models and human diseases. Mutations affecting these complexes are linked to various developmental disorders and cancer, highlighting their importance in genome stability and transcriptional regulation. Advances in model systems and genomic techniques have provided deeper insights into the pathological roles of SMC complex dysfunction, offering potential therapeutic avenues for associated diseases.
Low-Cost Electrochemical Determination of L-Ascorbic Acid Using Screen-Printed Electrodes and Development of an Electronic Tongue for Juice Analysis
Authors: El Anzi Laila , García María Soledad , Laborda Eduardo , Ruiz Alberto , Ortuño Joaquín Ángel
Published: November 2024

Low-cost electrochemical methodologies for the determination of L-ascorbic acid (vitamin C) and the analysis of juices are developed based on its electro-oxidation on carbon screen-printed electrodes. A novel chronoamperometric methodology is developed for the quantification of L-ascorbic acid in fruit juices. The proposed method stands out for its simplicity and rapidity, demonstrating its efficacy in determining L-ascorbic acid content in various fruit juices. Notably, the results obtained with this chronoamperometric approach are compared with those yielded by chromatography, with no significant differences between the two methods being found. Additionally, an electronic tongue is developed for the differentiation of juices based on the square wave voltammetric signals.
A Theoretical Examination of Various Complexes of a Proposed Novel Chemosensor Material—Graphene/SiC
Authors: Kalchevski Dobromir A. , Kolev Stefan , Dimov Dimitar , Trifonov Dimitar , Avramova Ivalina , Ivanova Pavlina , Milenov Teodor
Published: November 2024

The potential of semiconducting, corrugated graphene, grown on silicon carbide, as an active element in chemosensors is studied in the present work. For this purpose, the adsorption of benzene, diazepam and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the material’s surface was modeled. According to the graphene sheet bending and adsorbate–adsorbent distances, the heterostructure favors the ligands in the order of diazepam < benzene < TCDD. The apparent ambiguity in the results for diazepam is easy to explain. The abundance of lone pairs and π-electrons compensates for the low-symmetry, non-planar, far from optimal (adsorption-wise) geometry. The maximum band gap change in the heterostructure, caused by adsorption, is 0.02 eV. Intermolecular binding does not alter the HOMO–LUMO difference in benzene and TCDD by more than 0.01 eV. The completely planar molecules are not expected to undergo significant geometrical changes; hence, the alteration in their frontier orbitals is also minimal. The adsorption of diazepam, however, causes significant changes in the projected density of states of both structures in the complex. In conclusion, corrugated graphene is applicable as an active material in selective chemosensors for non-planar aromatic molecules.
Reimagining Kangaroo Care for Preterm Infants: A Novel Garment for Safe and Comfortable Bonding
Authors: McMillin Alexandra , Presser Aiden Aviva , Sherman Jules P. , Crystal Ruth Ann , Rhine William D.
Published: November 2024

Background/Objectives: Kangaroo Care (KC) has been proven to enhance physiological stability, growth, and bonding in preterm, low-birthweight infants. Despite its benefits, KC is underutilized in Level IV Neonatal Intensive Care Units (NICUs) due to challenges in managing medical equipment. This study introduces the Kangarobe™, a novel garment designed to facilitate safe, comfortable, and efficient KC for medically fragile infants in high-acuity NICUs. Methods: From 2021 to 2023, a feasibility study was conducted involving 25 infant-parent dyads in a Level IV NICU. The Kangarobe™ was designed using human-centered design principles and tested on infants dependent on respiratory support. Surveys employing a 5-point Likert scale were administered to parents and nursing staff to assess safety, comfort, ease of use, and procedural access. Results: Survey results showed positive feedback from both parents and nursing staff, particularly in the areas of safety and comfort. For example, 72–80% of parents and nurses responded positively regarding ease and comfort. High level of agreement (76%) on the security of medical line management, with minimal negative feedback. In addition, parents using the Kangarobe™ held their infants for an average of 171 min per session, with a notable increase compared to the typical 75 min, indicating enhanced comfort and feasibility for extended KC sessions. The Kangarobe™ successfully enabled the secure management of medical lines and tubes, with the vertical access window improving procedural efficiency without interrupting KC. Conclusions: The Kangarobe™ demonstrates promise in addressing barriers to KC in high-acuity NICUs. By enhancing safety, comfort, and ease of use, it supports wider adoption of KC practices, potentially improving patient safety, staff efficiency, and family-centered care.
Diagnosis and Treatment of Infantile Hemangioma from the Primary Care Paediatricians to the Specialist: A Narrative Review
Authors: Bellinato Francesco , Marocchi Maria , Pecoraro Luca , Zaffanello Marco , Del Giglio Micol , Girolomoni Giampiero , Piacentini Giorgio , Rigotti Erika
Published: November 2024

Infantile haemangiomas (IHs) affect 3–10% of infants, 10% of whom need topical or systemic beta-blocker therapy. Propranolol is the first choice for IHs with a high risk of complications. Since more than half of IHs leave a permanent mark, to reduce outcomes, it is essential to start oral propranolol (2–3 mg/kg/day in 2 doses/day) within the 5th month of life (i.e., during the proliferative phase) and to complete the therapy cycle for at least 6 months. This review aims to summarise the epidemiology, clinical presentation, diagnosis, and treatment of IHs and to highlight the importance of proper referral to specialised hub centres. Patients with vascular anomalies, particularly those suspected of having IH, should be referred to a specialised centre for accurate diagnosis, management by a multidisciplinary team, and timely treatment. IHs may pose life-threatening, functional, and aesthetic risks or may ulcerate. Segmental infantile haemangioma of the face/neck and the lumbosacral regions can be associated with various malformations. To ensure timely specialist evaluation and treatment to reduce the potential risk of complications, it is essential to identify high-risk IHs rapidly. The Infantile Haemangioma Referral Score (IHReS) scale is an important tool to assist primary care paediatricians and general dermatologists.
Enhanced Anti-Rollover Control for Commercial Vehicles Under Dynamic Lateral Interferences
Authors: Rong Jin , Wu Tong , Wang Junnian , Peng Jing , Yang Xiaojun , Meng Yang , Chu Liang
Published: November 2024

Commercial vehicles frequently experience lateral interferences, such as crosswinds or side slopes, during extreme maneuvers like emergency steering and high-speed driving due to their high centroid. These interferences reduce vehicle stability and increase the risk of rollover. Therefore, this study takes a bus as the carrier and designs an anti-rollover control strategy based on mixed-sensitivity and robust H∞ controller. Specifically, a 7-DOF vehicle dynamics model is introduced, and the factors influencing vehicle rollover are analyzed. Based on this, to minimize excessive intervention in the vehicle’s dynamic characteristics, the lateral velocity, roll angle, and roll rate are recorded at the vehicle’s rollover threshold as desired values. The lateral load transfer rate (LTR) is chosen as the evaluation index, and the required additional yaw moment is determined and distributed to the wheels for anti-rollover control. Furthermore, to verify the effectiveness of the proposed anti-rollover control strategy, a co-simulation platform based on MATLAB/Simulink and TruckSim is developed. Various dynamic lateral interferences (side winds with different changing trends and wind speeds) are introduced, and the fishhook and J-turn maneuvers are selected to analyze and compare the proposed control strategy with a fuzzy logic algorithm. The results indicate that the maximum LTR of the vehicle is reduced by 0.11. Additionally, the lateral acceleration and yaw rate in the steady state are reduced by more than 1.8 m/s² and 15°, respectively, enhancing the vehicle’s lateral stability.
Disability and Patient-Reported Satisfaction in Women with Idiopathic Intracranial Hypertension: A Comparative Study of Venous Sinus Stenting and Medical Management
Authors: Buhbut Ortal , Ben Assayag Hadas , Aharoni-Bar Sapir , Epstein Maor , Tsumi Erez , Regev Tamir , Bunin Anna , Honig Asaf , Kotaro Bar O. , Ben Arie Gal , Horev Anat
Published: November 2024

Objective: Patients with chronic idiopathic intracranial hypertension (IIH) commonly experience a high level of disability and low satisfaction with medical treatment. We aim to evaluate long-term functional improvement and patient satisfaction in IIH patients with similar symptoms by comparing venous sinus stenting (VSS) to standard medical therapy. Methods: We conducted a cross-sectional questionnaire study of 111 IIH patients, comparing 37 adult female patients who underwent venous sinus stenting with 74 patients treated medically. Propensity score matching was used to balance age and presence of papilledema at presentation between groups. Headache-related disability was evaluated using the Migraine Disability Assessment Scale (MIDAS), while general function and treatment satisfaction were assessed using custom questionnaires. Electronic medical records and the results of imaging upon diagnosis were reviewed retrospectively. Results: The stented group reported significantly better outcomes in physical well-being (median 4.0 vs. 1.0, p < 0.001), task completion (4.0 vs. 1.0, p < 0.001), work/school persistence (5.0 vs. 1.0, p < 0.001), and mental well-being (4.0 vs. 1.0, p < 0.001). Additionally, the stented group had a lower proportion of patients with severe MIDAS (MIDAS > 4, 24.3% vs. 47.9%, p = 0.017). Logistic regression suggested venous stenting as a protective factor against severe MIDAS scores (OR = 0.174, p = 0.004). Conclusion: Cerebral venous stenting in patients with IIH is associated with lower disability and higher patient satisfaction from medical treatment compared to those treated with medications only. These findings suggest that venous sinus stenting may be a valuable treatment option for selected IIH patients. However, larger prospective studies are needed to further validate our results.
Deciphering the Role of Androgen in the Dermatologic Manifestations of Polycystic Ovary Syndrome Patients: A State-of-the-Art Review
Authors: Taieb Ach , Feryel Amri
Published: November 2024

Polycystic ovary syndrome is a presvalent endocrine disorder affecting women globally, characterized by hormonal imbalance, metabolic disturbances, and reproductive dysfunction. Diagnosis relies on clinical evaluation, medical history assessment, physical examination, and laboratory tests, with the Rotterdam criteria being widely used. The pathophysiology of PCOS involves genetic predisposition, environmental factors, and lifestyle influences, with hormonal dysregulation, particularly elevated androgens, insulin resistance, and chronic inflammation, playing a pivotal role. These mechanisms not only contribute to reproductive and metabolic disturbances but also to the various cutaneous manifestations, such as acne, hirsutism, and alopecia. This review aims to analyze the role of androgens in the dermatological manifestations in patients with polycystic ovary syndrome, providing insights into underlying mechanisms and guiding the development of effective therapeutic strategies. By synthesizing available evidence, this review aims to deepen understanding of related dermatological manifestations and improve outcomes for affected individuals.
The Utility of Pre-Procedural Blood Tests in Neuraxial Blocks: A Retrospective Study in High-Risk Patients
Authors: Moon Sungho , Oh Daeseok
Published: November 2024

Background/Objectives: The necessity and clinical utility of routine pre-procedural blood tests (PBTs) before neuraxial blockade remain controversial. This study evaluates the effectiveness of PBTs in identifying clinically significant conditions in an outpatient setting. Methods: This single-center retrospective study involved patients who received neuraxial blockades from January 2020 to August 2023. We extracted medical information and laboratory data from the electronic medical records during the pre-procedural period. Through a multivariate regression analysis, we identified patient factors associated with abnormal laboratory results. Results: Advanced age (OR, 1.021; p = 0.026) and a history of cancer (OR, 2.359; p = 0.016) were significantly associated with elevated CRP levels (>0.30 mg/dL). Severe hyperglycemia (≥200 mg/dL) was found in 24 patients (3.88%), with a history of cancer being a strong predictor (OR, 6.764; p < 0.001). No significant abnormalities were observed in PT or PTT. Reduced eGFR (<60 mL/min/1.73 m2) was detected in 8.62% of patients, despite no prior history of renal dysfunction. A multivariate analysis revealed that advanced age, hypertension, cancer, and coronary artery disease were significant predictors of abnormal PBT results, highlighting the importance of selective testing in high-risk patients. Conclusions: Routine PBTs are not universally required for all patients undergoing neuraxial blockade but can provide crucial information in high-risk populations. A selective testing approach based on individual risk factors is recommended to optimize patient safety and resource utilization. Future studies should aim to establish clear guidelines for targeted PBT use.
The Promotion of Non-Communicable Disease Screening in Gurage Zone, Ethiopia: A Mixed-Method Study
Authors: Debebe Heldana , Ketema Bezawit , Rossner Sophie Sarah , Negash Sarah , Addissie Adamu , Kaba Mirgissa , Tamire Mulugeta , Kantelhardt Eva Johanna
Published: November 2024

Background: Despite the high non-communicable disease (NCD) mortality in Ethiopia, NCD screening in the country remains suboptimal. This study assessed the health communication process and materials designed to promote NCD screening among adult primary healthcare facility attendants in the Gurage Zone, Ethiopia. Methods: A parallel mixed-methods approach was employed. Seven health communication materials were evaluated using the Modified Clear Communication Index Score by the Center for Disease Control and Prevention (CDC). Seven key informants who were involved in the production were interviewed to explore the process. Users’ satisfaction with the materials was assessed through a cross-sectional survey (N = 412). We used multivariable logistic regression with SPSS version 27 software to determine the factors associated with users’ satisfaction. Thematic analysis was applied for the qualitative data analysis using Opencode 4.03 software. Results: Qualitative interviews indicated that the production process relied on evidence, involved stakeholders, and included pretesting. The CDC index score revealed that five of the seven materials were considered clear and user-friendly, whereas two required improvement. Overall users’ satisfaction with the material was high with a mean score of 22.10 (SD ± 2.34; min: 14, max: 25). Age and educational status had significant positive association with users’ satisfaction. Conclusions: Developing health communication materials on promoting NCD screening based on evidence, stakeholders’ input, and pretesting can lead to good quality material and user satisfaction. We recommend future research works to measure changes in NCD screening service uptake as a result of using the health communication materials considered in this study.
Advancements in Soybean Price Forecasting: Impact of AI and Critical Research Gaps in Global Markets
Authors: Mello Fernando Dupin da Cunha , Kumar Prashant , Sperandio Nascimento Erick G.
Published: November 2024

Soybeans, a vital source of protein for animal feed and an essential industrial raw material, are the most traded agricultural commodity worldwide. Accurate price forecasting is crucial for maintaining a resilient global food supply chain and has significant implications for agricultural economics and policymaking. This review examines over 100 soybean price forecast models published in the last decade, evaluating them based on the specific markets they target—futures or spot—while highlighting how differences between these markets influence critical model design decisions. The models are also classified into AI-powered and traditional categories, with an initial aim to conduct a statistical analysis comparing the performance of these two groups. This process unveiled a fundamental gap in best practices, particularly regarding the use of common benchmarks and standardised performance metrics, which limits the ability to make meaningful cross-study comparisons. Finally, this study underscores another important research gap: the lack of models forecasting soybean futures prices in Brazil, the world’s largest producer and exporter. These insights provide valuable guidance for researchers, market participants, and policymakers in agricultural economics.
Cost Analysis of Penitentiary Systems and Comparison Between the Countries of the Council of Europe
Authors: Altobelli Emma , Guergache Antonello Karim , Galassi Francesca , Petrocelli Reimondo , Marziliano Ciro
Published: November 2024

Background: The objective was to analyze the budgets invested in prisons by the member states of the Council of Europe (CoE) and the relationships between the global cost, the cost incurred per single inmate, the number of inmates per 100,000 inhabitants (PPR), the gross domestic product (GDP) and per capita GDP. Methods: The data relating to the variables considered for the year 2020 were obtained from the SPACE-I 2021 of the CoE, the World Bank/OECD, and Eurostat. Regression models were used to evaluate the relationships between the PPR and the GDP, the daily cost per prisoner and per capita GDP, and between the PPR and the per capita GDP. A multiple correspondence analysis was performed to evaluate associations between the PPR, EU membership, cost per day, cost rate, geographical area, and inmate gender. Results: The daily expenditure per inmate in northern European countries reaches very high values, respectively: EUR 330.6 (Norway) and EUR 303 (Sweden), while, in the eastern countries, the values drop sharply (EUR 6.50 in Bulgaria and EUR 8.08 in Azerbaijani). The lowest PPR values are found in northern European countries, and the highest in the following countries: Russia, Turkey, Georgia, and Azerbaijan. Conclusions: Countries with a higher GDP per capita tend to have lower prison population rates and to invest larger amounts of funds for prison systems.
Derivative Markets and Economic Growth: A South African Perspective
Authors: Stevens Matthew , Vermeulen Cobus
Published: November 2024

It is well established that financial development and innovation promote economic growth through improving the allocation of capital, enhancing risk management, contributing to price discovery, and increasing market efficiencies. While a vast empirical literature is devoted to the nexus between financial development and economic growth, however, substantially less research has been done on the relationship between derivatives and growth, especially in the emerging-market context. Derivatives can be viewed as a specific category of financial innovation, which may advance economic growth through its specialised functions of risk management and price discovery. This paper contributes to bridging this gap in the literature by exploring the impact of exchange-traded futures derivatives on South African economic growth, output, and economic growth volatility. It employs ARDL bounds tests, Granger causality tests and GARCH volatility modeling to analyse the effects of exchange-traded futures derivatives on various measures of South African economic activity. The main result is that exchange-traded futures derivatives contribute positively to South African economic growth and economic activity. This may suggest that opportunities might exist in other emerging economies, with financial structures comparable to that of South Africa, to encourage the development of organised and well-regulated derivatives markets to unlock economic growth in these economies.
Market-Driven Mapping of Technological Advancements in the Seafood Industry: A Country-Level Analysis
Authors: Subash Abhirami , Ramanathan Hareesh N. , Šostar Marko
Published: November 2024

Seafood preservation techniques have evolved from ancient methods to modern innovations like canning, freezing, and surimi production. Canning in the 19th century introduced airtight containers, while commercial freezing technologies like flash freezing extended shelf life. Surimi pastes in the 20th century led to affordable imitation seafood products. Emerging technologies continue to enhance seafood preservation methods. Moreover, the integration of digital technology, automation, and data sharing, known as Industry 4.0, is transforming various industries. This integration encompasses blockchain technology, automation, robotics, and big data analytics, aiming to enhance production, sustainability, traceability, and efficiency in fish processing. With a focus on the seafood market dynamics affecting these advances, this research was conducted with the aim to understand how technical breakthroughs in the seafood business are dispersed and implemented across different nations. We aim to determine the correspondence between the technological sophistication of machinery in seafood processing companies and map it across different countries across the globe to obtain an understanding of the generation of technology used in prominence. Variations in adoption rates and technological trends reflect regional market dynamics. The Seafood Expo ASIA 2023 study looked at the use of Industry 4.0 technologies, operational procedures, and technology adoption in the global seafood processing industry. Notably, countries like Norway, the Republic of Korea, Spain, Turkey, and the Netherlands have rapidly embraced Industry 4.0 technologies. The market factors driving these technological advancements across different countries include rising consumer demand for sustainable seafood, economic incentives, and global competition. A correspondence analysis was employed to analyze the correspondence between countries and the level of technological sophistication in the machinery used. We successfully mapped the level of technology utilized in machinery across global seafood processing companies, providing insights into the technological advancements shaping the industry.
Microplastics in Urban Ambient Air: A Rapid Review of Active Sampling and Analytical Methods for Human Risk Assessment
Authors: Han Inkyu , Lee Chanmi , Belchez Caesar , Shipper Andrea Goldstein , Wiens Kirsten E.
Published: November 2024

This study conducted a rapid review to evaluate active air sampling and analytical methods for characterizing outdoor air microplastics in urban areas. We synthesized information from 35 peer-reviewed journal articles. Studies utilizing active sampling methods were able to provide detailed data on inhalation concentrations and doses. The analytical techniques reviewed were categorized into microscopy, Fourier Transform Infrared (FTIR) spectroscopy, Raman spectroscopy, scanning electron microscopy (SEM), and mass spectrometry, including pyrolysis–gas chromatography (Py-GC). While conventional FTIR and Raman spectroscopy can identify microplastics in total suspended particles, advanced instruments such as µRaman and SEM are crucial for analyzing inhalable microplastics (e.g., particles smaller than 10 µm). Characterizing the shapes and colours of microplastics can provide qualitative estimates of their sources, with fibres and the colour black being the most predominant characteristics. Establishing dose–response relationships for health effects requires quantitative analyses; thus, combining techniques like µRaman with Py-GC is essential for comprehensive human risk assessments. Future studies should focus on identifying and quantifying inhalable microplastic compounds that are relevant to human health.
An Analysis and Comparison of the Hydrodynamic Behavior of Ships Using Mesh-Based and Meshless Computational Fluid Dynamics Simulations
Authors: Caccavaro Davide , Tagliafierro Bonaventura , Bilotta Gianluca , Domínguez José M. , Caravella Alessio , Gaudio Roberto , Cassano Alfredo , Altomare Corrado , Lauria Agostino
Published: November 2024

This paper presents a comparison of two turbulence models implemented in two different frameworks (Eulerian and Lagrangian) in order to simulate the motion in calm water of a displacement hull. The hydrodynamic resistance is calculated using two open-source Computational Fluid Dynamics (CFD) software packages: OpenFOAM and DualSPHysics. These two packages are employed with two different numerical treatments to introduce turbulence closure effects. The methodology includes rigorous validation using a Wigley hull with experimental data taken from the literature. Then, the validated frameworks are applied to model a ship hull with a 30 m length overall (LOA), and their results discussed, outlining the advantages and disadvantages of the two turbulence treatments. In conclusion, the resistance calculated with OpenFOAM offers the best compactness of results and a shorter simulation time, whereas DualSPHysics can better capture the free-surface deformations, preserving similar accuracy.
Genotoxic and Anti-Genotoxic Assessments of Fermented Houttuynia cordata Thunb. Leaf Ethanolic Extract and Its Anti-Cancer Effect in a Dual-Organ Carcinogenesis Model of Colon and Liver in Rats
Authors: Singai Chonikarn , Pitchakarn Pornsiri , Taya Sirinya , Wongpoomchai Rawiwan , Wongnoppavich Ariyaphong
Published: November 2024

The incidence of multiple-organ cancers has recently increased due to simultaneous exposure to various environmental carcinogens. Houttuynia cordata Thunb. (H. cordata) is recognized for its many health benefits, including its anti-cancer properties. The fermentation of its leaves has been shown to significantly enhance the bioflavonoid content and its bioactivities. This study aimed to evaluate the effectiveness of fermented H.cordata leaf (FHCL) extracts against combined carcinogens and investigate the underlying mechanisms. The crude ethanolic extract of FHCL was partitioned to obtain hexane- (HEX), dichloromethane- (DCM), ethyl acetate- (ETAC), butanol- (nBA), and residue fractions. The crude ethanolic extract (200–250 μg/mL) and the DCM fraction (50 μg/mL) significantly reduced NO production in RAW264.7 macrophages. In addition, the crude extract and the DCM and ETAC fractions showed anti-genotoxicity against aflatoxin B1 and 2-amino-3,4-dimethylimidazo [4,5-f]quinoline (MeIQ) in Salmonella typhimurium assays (S9+). Despite demonstrating genotoxicity in the Salmonella mutation assay (with and without S9 activation), oral administration of the crude extract at 500 mg/kg of body weight (bw) for 40 days in rats did not induce micronucleated hepatocytes, indicating that the extract is non-genotoxic in vivo. Moreover, the crude extract significantly decreased Phase I but increased Phase II xenobiotic-metabolizing enzyme activities in the rats. Next, the anti-cancer effects of FHCL were evaluated in a dual-organ carcinogenesis model of the colon and liver in rats induced by 1,2-dimethylhydrazine (DMH) and diethylnitrosamine (DEN), respectively. The crude extract significantly reduced not only the number and size of glutathione S-transferase placental form positive foci in the liver (at doses of 100 and 500 mg/kg bw) but also the number of aberrant crypt foci in rat colons (at 500 mg/kg bw). Furthermore, FHCL significantly reduced the expression of proliferating cell nuclear antigen (PCNA) in the colon (at 100 and 500 mg/kg bw) and liver (at 500 mg/kg bw) of the treated rats. In conclusion, FHCL exhibits significant preventive properties against colon and liver cancers in this dual-organ carcinogenesis model. Its mechanisms of action may involve anti-inflammatory effects, the prevention of genotoxicity, the modulation of xenobiotic-metabolizing enzymes, and the inhibition of cancer cell proliferation. These findings support the use of FHCL as a natural supplement for preventing cancer.
Ultraviolet-B Radiation Stimulates Flavonoid Biosynthesis and Antioxidant Systems in Buckwheat Sprouts
Authors: Tian Xin , Hu Meixia , Yang Jia , Yin Yongqi , Fang Weiming
Published: November 2024

Abiotic stress not only elevates the synthesis of secondary metabolites in plant sprouts but also boosts their antioxidant capacity. In this study, the mechanisms of flavonoid biosynthesis and antioxidant systems in buckwheat sprouts exposed to ultraviolet-B (UV-B) radiation were investigated. The findings revealed that UV-B treatment significantly increased flavonoid content in buckwheat sprouts, with 3-day-old sprouts exhibiting a flavonoid content 1.73 times greater than that of the control treatment. UV-B radiation significantly increased the activities of key enzymes involved in flavonoid biosynthesis (phenylalanine ammonia-lyase, 4-coumarate-CoA ligase, cinnamate 4-hydroxylase, and chalcone synthase) and the relative expression levels of the corresponding genes. Although UV-B radiation caused damage to the cell membranes of buckwheat sprouts, promoting increases in hydrogen peroxide and malondialdehyde content and inhibiting the growth of sprouts, importantly, UV-B radiation also significantly increased the activities of catalase, peroxidase, and superoxide dismutase as well as the relative expression levels of the corresponding genes, thus enhancing the antioxidant system of buckwheat sprouts. This enhancement was corroborated by a notable increase in ABTS, DPPH, and FRAP radical scavenging activities in 3-day-old sprouts subjected to UV-B radiation. Additionally, UV-B radiation significantly increased chlorophyll a and chlorophyll b contents in sprouts. These results suggest that UV-B radiation is advantageous for cultivating buckwheat sprouts with increased flavonoid content and enhanced antioxidant capacity.
Motivators of the Intention of Wine Tourism in Baja California, Mexico
Authors: Leyva-Hernández Sandra Nelly , Toledo-López Arcelia
Published: November 2024

The present study aimed to analyze which variables have the most significant effect and importance in analyzing wine tourism intention in Baja California, Mexico, using the stimulus–organism–response model. An exploratory and cross-sectional study collected a sample of 728 adult wine consumers from Baja California. Data analysis was carried out using structural equation modeling by partial least squares and analysis of the importance–-performance map. Traditional gastronomy was the main predictor of wine tourism intention in Baja California, Mexico, and according to the importance–performance map, it is the most essential variable in the analysis. In addition, the study results showed that identity mediates the relationship between electronic word of mouth and wine tourism intention and between traditional gastronomy and wine tourism intention. The study employs an importance–performance mapping analysis that has yet to be used in wine tourism analysis and proposes a stimulus (electronic word of mouth, traditional gastronomy)–organism (identity)–response (wine tourism intention) model to broaden understanding of the phenomenon. Its findings and methodology can serve as a valuable template for future research, offering a blueprint that can be replicated in regions like Baja California. This research has significant consequences for creating marketing plans in the wine tourism sector.
Effects of Red Vinasse on Physicochemical Qualities of Blue Round Scad (Decapterus maruadsi) During Storage, and Shelf Life Prediction
Authors: Xue Shan , Chen Shuyi , Liu Bohu , Liu Jia
Published: November 2024

A fish processed with red vinasse is a type of Fujian cuisine with regional characteristics. In order to monitor the effect of red vinasse on storage quality and shelf life of blue round scad (Decapterus maruadsi) during storage, the changes in fat content, thiobarbituric acid reactive substances (TBARS), composition of polyunsaturated fatty acids (PUFAs), pH value, texture, and sensory quality were studied at different storage temperatures (4 °C, 25 °C, and 37 °C). By analyzing the correlation between changes in sensory qualities and physical and chemical indexes, a first-order kinetic model and the Arrhenius equation were used to build a shelf-life prediction model for blue round scad during storage. The results showed that processing with red vinasse can significantly reduce the malondialdehyde (MDA) production and the decrease in PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (p < 0.05) during storage. Based on partial least squares regression (PLSR), the storage temperature and time have a significant impact on the PUFA composition in blue round scad, which changed less when the samples were stored at 4 °C and 25 °C, and they had better nutritional composition of fatty acids at lower temperatures. Among the PUFAs, DHA (C22:6n-3) and EPA (C20:5n-3) had higher relative contents and significantly decreased during storage (p < 0.05). Additionally, the processing with red vinasse can slow down the increase in total volatile basic nitrogen (TVB-N) value and pH of blue round scad, maintain the appropriate hardness, elasticity, cohesion and chewability, and improve the overall sensory quality of the fish. In addition, according to the results of model prediction based on TBARS value, the storage shelf life of blue round scad with red vinasse added was 55 d, 2.7 d and 28 h at 4 °C, 25 °C and 37 °C, respectively. The accuracy of the forecast model was high, and the relative errors of the measured values and predicted values were less than 10%. Thus, it not only provided a theoretical basis for the processing and application of red vinasse to Chinese traditional food, but also provided innovative ideas for the safe storage and high-value utilization of blue round scad.
Modulation of Serum Metabolic Profiles by Bifidobacterium breve BBr60 in Obesity: A Randomized Controlled Trial
Authors: Wu Ying , Gao Dejiao , Pan Yujia , Dong Yao , Bai Zhouya , Gu Shaobin
Published: November 2024

Obesity, a prevalent metabolic disorder in youth, leads to complications and economic strain. Gut dysbiosis significantly contributes to obesity and metabolic issues. Bifidobacterium breve, a probiotic strain, may help regulate gut dysbiosis and benefit obese individuals. However, more research is needed on its effect on serum metabolism. A total of 75 overweight or obese young adults (aged 19–45) participated in the current study, and were randomly divided into probiotic and placebo groups using a random number table. Both groups received dietary guidance and underwent twelve weeks of intervention with either oral Bifidobacterium breve BBr60 (BBr60) or a placebo. After the intervention, collection and analysis of fasting serum samples were conducted using mass spectrometry coupled with liquid chromatography. Analyses of associations were conducted in order to determine the correlations between key serum metabolites and clinical obesity indicators, aiming to understand the influence of BBr60. Due to 10 participants dropping out for personal reasons, the study included 32 and 33 participants in the placebo and the BBr60 groups, respectively. The BBr60 intervention significantly regulated 134 serum metabolites and influenced crucial metabolic pathways in obesity management (p < 0.05), including ascorbate and aldarate metabolism for oxidative stress reduction, cholesterol metabolism for lipid regulation, parathyroid hormone synthesis, secretion and action for the endocrine system, oxidative phosphorylation for enhanced energy efficiency, and glycolysis/gluconeogenesis for glucose metabolism. Analysis showed a positive relationship between fasting blood glucose (FBG), aspartate aminotransferase (AST), total protein (TP), and the content of 5-Methyl DL-glutamate (p < 0.05). Similarly, body mass index (BMI), weight, and body fat percentage (BFP) were positively linked to serum metabolites (1-Hydroxycyclohexyl) acetic acid, and 5-Oxooctanoic acid (p < 0.05). Significant associations of AST levels with key serum metabolites in cholesterol metabolism pathways further suggest BBr60’s potential to improve liver function and overall metabolic health in overweight or obese individuals. These findings support BBr60’s effectiveness in modulating serum metabolic profiles and suggest it may improve liver function and BMI in overweight or obese individuals by regulating key serum metabolites.
Chemical Composition, Bioactivities, and Applications of Spirulina (Limnospira platensis) in Food, Feed, and Medicine
Authors: Spínola Maria P. , Mendes Ana R. , Prates José A. M.
Published: November 2024

Spirulina (Limnospira platensis) is a microalga recognised for its rich nutritional composition and diverse bioactive compounds, making it a valuable functional food, feed, and therapeutic agent. This review examines spirulina’s chemical composition, including its high levels of protein, essential fatty acids, vitamins, minerals, and bioactive compounds, such as the phycocyanin pigment, polysaccharides, and carotenoids, in food, feed, and medicine. These compounds exhibit various biological activities, including antioxidant, anti-inflammatory, immunomodulatory, antiviral, anticancer, antidiabetic and lipid-lowering effects. Spirulina’s potential to mitigate oxidative stress, enhance immune function, and inhibit tumour growth positions it as a promising candidate for preventing chronic diseases. Additionally, spirulina is gaining interest in the animal feed sector as a promotor of growth performance, improving immune responses and increasing resistance to diseases in livestock, poultry, and aquaculture. Despite its well-documented health benefits, future research is needed to optimize production/cultivation methods, improve its bioavailability, and validate its efficacy (dose–effect relationship) and safety through clinical trials and large-scale human trials. This review underscores the potential of spirulina to address global health and nutrition challenges, supporting its continued application in food, feed, and medicine.
An Exploratory Pilot Qualitative Study That Explores the Influences on Mental Health and Well-Being in Indigenous Youth and Young Adults
Authors: Zuffante Mona J.
Published: November 2024

Background: Suicide is the second leading cause of death among American Indian (AI) adolescents and young adults in the 15- to 24-year-old age group and is the third leading cause of death in the 10- to 14-year-old age group. Methods: Key informant interviews were conducted with AI youth (n = 10) ages 12–18, and young AI adults (n = 10) ages 19–24 to gather input on activities and programs to decrease AI suicidal-related behaviors in Nebraska. These interviews were 45 min in length at maximum. Themes were created once the interviews were completed. Results: The overarching theme was creating and implementing more suicide prevention programs and cultural activities for these age groups. Respondents identified three important characteristics that they believe all programs should have: (1) positive reinforcement, (2) culturally-centered activities, and (3) strength-based approaches that are not from a negative or punitive viewpoint. Conclusion: The results from these interviews can be used to build strengths-based approaches to promoting positive mental health in Indigenous communities and can lead to other successful programs and activities.
Mechanistic Role of TRIM26 in Viral Infection and Host Defense
Authors: Sharma Mona , Liu Ke , Wei Jianchao , Ma Zhiyong , Qiu Yafeng
Published: November 2024

Tripartite motif protein 26 (TRIM26) is an E3 ubiquitin ligase and a member of the TRIM family. Similar to other TRIM proteins, TRIM26 consists of three domains, collectively termed RBCC: a Really Interesting New Gene (RING) domain, one B-Box domain, and a C terminal domain consisting of a PRY/SPRY domain. The PRY/SPRY domain exhibits relatively higher conservation compared with the RING and B-Box domains, suggesting potentially similar roles across TRIM26 proteins from various species. TRIM26 either directly interacts with viral proteins or modulates immune responses to engage with a viral infection, serving as either a protective or detrimental host factor depending on the circumvent of the viral infection. The present review focuses on understanding the mechanisms of TRIM26 during viral infection and its potential future applications.
Nucleotide and Amino Acid Analyses of Unique Infectious Bronchitis Virus (IBV) Variants from Canadian Poultry Flocks with Drop in Egg Production
Authors: Farooq Muhammad , Ali Ahmed , Hassan Mohamed S. H. , Abdul-Careem Mohamed Faizal
Published: November 2024

Background/Objectives: Infectious bronchitis (IB) is a highly infectious avian disease caused by the infectious bronchitis virus (IBV). The disease causes lesions mainly in the respiratory, reproductive, and renal systems and has a significant economic impact on the poultry industry worldwide. Methods: We discovered two unique IBV isolates (T-62: PP737794.1 and CL-61: PP783617.1) circulating in Canada and molecularly characterized them. Results: The phylogenetic analysis revealed that the IBV isolates belong to genotype I and fall between lineages 25 and 7. Further analysis of the T-62 IBV isolate indicated that it is a potential recombinant of the Iowa state isolate (IA1162/2020-MW) and that the CL-61 strain of the IBV is also a recombinant IBV with the Connecticut (Conn) vaccine strain as its major parent. The S1 glycoprotein of the CL-61 and T-62 strains of the IBV had 85.7% and 73.2% amino acid (aa) identities respectively compared to the Conn vaccine strain. There were 67 and 129 aa substitutions among the S1 glycoprotein of the CL-61 and T-62 strains of the IBV compared to the Conn vaccine, respectively. Importantly, two and nineteen of these aa variations were in hypervariable regions 1 (HVR1) and HVR3. Finally, the two IBV isolates possessed a higher affinity for the sialic acid ligand compared to the DMV/1639 and Mass/SES IBV strains. Conclusions: Genetic recombination in the IBV results in the continual emergence of new variants, posing challenges for the poultry industry. As indicated by our analyses, live attenuated vaccine strains play a role in the genetic recombination of the IBV, resulting in the emergence of variants.
L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme
Authors: Sakimoto Nae , Imanaka Hirofumi , Tomita-Sudo Elisa , Akita Tomoka , Kawakami Junji
Published: November 2024

Background/Objectives: Riboswitches are functional nucleic acids that regulate biological processes by interacting with small molecules, such as metabolites, influencing gene expression. Artificial functional nucleic acids, including deoxyribozymes, have been developed through in vitro selection for various catalytic functions. In a previous study, an l-histidine-dependent deoxyribozyme was identified, exhibiting RNA cleavage activity in the presence of l-histidine resembling ribonuclease catalytic mechanisms. This study aims to clarify the role of l-histidine in the activity and structural formation of the l-histidine-dependent deoxyribozyme (HD), focusing on the binding properties and conformational changes of its derivative HD3. Methods: Conformational changes in HD3 were analyzed using circular dichroism (CD) under varying concentrations of l-histidine. Direct binding analysis was conducted using carbon-14 (14C)-labeled l-histidine and a liquid scintillation counter. The catalytic activity of HD3 in the presence of different l-histidine concentrations was measured. Results: The binding constant for l-histidine-induced conformational changes (Ka(CD)) was found to be 2.0 × 103 (M−1), whereas for catalytic activity (Ka(Rxn)) and scintillation counting (Ka(RI)), it was approximately 1.0 × 103 (M−1). Conclusions: l-Histidine plays an essential role in both the catalytic activity and structural formation of the HD3 deoxyribozyme. The consistent binding constants across different experimental methods highlight the significant contribution of l-histidine to the active folding of deoxyribozymes.
Gloria Anzaldúa’s New Mestiza Consciousness Through Kristevan Female Writing and the Re-Shaping of Divine Maternal Archetypes
Authors: Wang Yuanjiang
Published: November 2024

Faced with the hegemony of racial superiority, the oppression of gender dominance, and the demands of religious homogeneity, Mexican American Gloria E. Anzaldúa proposes a New Mestiza Consciousness that seeks to achieve a multifaceted transcendence of La Frontera (Borderlands). Using Krsiteva’s semiotics and mythology-based feminism as a theoretical guide, this paper will analyze the cultural, gender, ethnic, and religious manifestations of New Mestiza Consciousness and the logic behind this consciousness in terms of women’s writing in the Chicana women’s literary community and the re-shaping of the maternal mythological archetype in indigenous culture.