bluuebunny/crossref_metadata_2025 huggingface dataset:
Digital Marketing dan Desain Kemasan bagi Petani Milenial Program YESS
Authors: Sudirwo  , Santoso Budi , Tri Aditia Permana Angga
Published: October 2024

Studi ini membahas kegiatan pengabdian yang fokus pada Digital Marketing dan Desain Kemasan bagi Petani Milenial dalam Program YESS. Melalui analisis situasi, ditemukan bahwa petani milenial membutuhkan pengetahuan tentang pemasaran digital dan desain kemasan untuk meningkatkan penjualan produk pertanian mereka. Metode pelatihan yang digunakan meliputi ceramah, demonstrasi, diskusi, dan praktik. Hasil kegiatan pelatihan dilakukan dengan baik di Dinas Pertanian Kabupaten Banjar dengan peserta sebanyak 25 orang, dengan narasumber yang kompeten dalam bidang digital marketing dan desain kemasan. Peserta pelatihan mampu menguasai materi dan menunjukkan semangat untuk melanjutkan pembelajaran. Dari hasil diskusi dan praktik, peserta merasa terbantu dan senang dengan kegiatan ini. Secara keseluruhan, kegiatan ini berhasil mencapai tujuan pengabdian dengan baik, membantu peserta dalam mengembangkan kemampuan digital marketing dan desain kemasan produk untuk meningkatkan usaha pertanian mereka.
Influencia Educativa en la Era Digital: Puntos Clave desde la Perspectiva de la Docencia 5.0
Authors: Saborío-Taylor Silvia
Published: October 2024

La era digital ha revolucionado el ámbito educativo, dando paso a la docencia 5.0, un enfoque que va más allá de la simple integración tecnológica para ofrecer una educación más humana y centrada en el desarrollo integral del estudiantado. Este enfoque se basa en tres claves fundamentales: la esencia de la docencia 5.0, que implica el uso de herramientas digitales para promover una educación más humana y enfocada en el desarrollo socioemocional del alumnado, con un énfasis en la innovación, la colaboración y la adaptabilidad del profesorado como facilitador del aprendizaje en un entorno en constante evolución. El papel influyente de la docencia 5.0, donde las personas docentes utilizan tecnologías digitales para inspirar y motivar tanto a estudiantes como a colegas, liderando el cambio educativo y creando ambientes de aprendizaje colaborativos y estimulantes. Los medios de soporte para consolidar la docencia 5.0 como influencers educativos, utilizando redes sociales, blogs, pódcast y conferencias, así como la creación y compartición de recursos innovadores, que facilitan el intercambio de ideas y la colaboración, enriqueciendo el entorno educativo. En resumen, la docencia 5.0 tiene el potencial de impactar positivamente en la educación, al fomentar un aprendizaje dinámico y colaborativo, guiando una transformación hacia un futuro más centrado en el estudiantado. No obstante, una práctica docente influyente guía y motiva a través de conocimientos valiosos, pero es vital que se enfoque en un impacto positivo y en la adaptación a la brecha digital, metodologías inclusivas y sostenibilidad de este nuevo enfoque.
Pódcast: una experiencia innovadora de enseñanza-aprendizaje en las personas adolescentes de secundaria de Antofagasta para reflexionar sobre sus vivencias post-COVID
Authors: Mendiz Rojas Heleny , Di Cintio Antonella , Romero Jeldres Marcela
Published: October 2024

Este artículo presenta una experiencia docente de buenas prácticas e innovación que explora el uso del pódcast como una estrategia pedagógica en el proceso de enseñanza-aprendizaje. La experiencia consistió en la creación de varios pódcast por parte de las personas estudiantes de octavo año básico de la ciudad de Antofagasta en Chile, permitiéndoles expresar sus experiencias y reflexiones tras el confinamiento por COVID-19. La metodología se implementó a través de un taller extraescolar voluntario, realizado intensivamente del 7 al 11 de noviembre de 2022. Las personas estudiantes no solo debían conocer los elementos que componen el lenguaje radial para crear pódcast, sino que también debían aprender a editar sus producciones radiofónicas, escuchar y comentar en grupo sus pódcast, con la finalidad de que pudieran comprender la utilidad de este formato en sus propios procesos. De ese modo, ellos se familiarizaron con el formato radial, crearon guiones, grabaron, editaron y escucharon sus propias producciones, que resultaron en nueve pódcast. A pesar de que los temas iniciales experimentaron variaciones, la actividad fue valorada positivamente por el estudiantado, lo que confirmó su efectividad. Por tanto, esta experiencia se convirtió en un aporte significativo para el cambio educativo, proporcionando a las personas estudiantes una nueva herramienta para producir sus propios contenidos. Lo anterior permite concluir que esta herramienta puede ser utilizada como recurso tanto por docentes como por el alumnado, a partir de un proceso de planificación didáctica. Sin embargo, es crucial tener en cuenta que los pódcast deben ser impulsados por la motivación intrínseca de las personas estudiantes, evitando una perspectiva externa y adultocéntrica.
Rasgos del perfil profesional del profesorado universitario influencer: voces de la población estudiantil
Authors: Corrales- Escalante Xinia , Hernández-Chaves Carolina
Published: October 2024

Este trabajo investigativo es acerca de las características que debe tener un docente universitario para ser considerado influencer educativo desde la perspectiva del estudiantado perteneciente a la generación Z. La población de estudio la conforman 23 estudiantes de la carrera Administración de Oficinas y 10 estudiantes de la carrera Educación Comercial de la Escuela de Secretariado Profesional, de la Facultad de Ciencias Sociales, Universidad Nacional, Costa Rica, a quienes se les realizó una entrevista para obtener información empírica y develar la opinión de cada participante, garantizando el anonimato. El enfoque metodológico que se utiliza en este estudio es de corte cualitativo y considera un diseño exploratorio con el propósito de realizar una interpretación contextual de la realidad y comprender el objeto de estudio, a partir de las vivencias, los aprendizajes y los comportamientos del estudiantado en los cursos universitarios.  El principal resultado de esta investigación evidenció la naturaleza multifacética del docente influencer universitario, quien requiere tener un equilibrio entre conocimientos técnicos, habilidades blandas, un profundo compromiso con el proceso de enseñanza y aprendizaje apoyado en las tecnologías digitales y las redes sociales para contribuir en una formación integral del estudiantado. Se concluye que el estudiantado destaca características intrínsecas y personales del docente universitario influencer, tales como la motivación, la empatía, la paciencia y la pasión por la enseñanza, así como los conocimientos y la mediación pedagógica, aspectos que van más allá de una presencia en redes sociales.
La competencia mediática en los perfiles pedagógicos de la profesora Alba Marília
Authors: Roche Aléxia , De Mello Kaam Tágides Renata , De Almeida Pimenta Maria Alzira
Published: October 2024

Media literacy is a challenge and a necessity for teacher education because media content is present in everyday school and social life. Moreover, it is often used or accessed without the necessary knowledge. Therefore, media literacy is fundamental for people to be able to consume and produce content critically and analytically. The aim of this study was to understand the production of content in social media carried out by Professor Alba Marília and its relationship with media literacy. For this purpose, a netnography study was conducted to analyze her most popular YouTube videos and Instagram stories. The comments on the videos in both social networks were analyzed, which, like the interviews, served as a basis for understanding how Ferrés and Piscitelli's (2012) dimensions of media literacy can be observed in the professor's profiles. The results show that the teachers' videos make some dimensions of media literacy explicit, such as language and technology. It can be concluded that Alba Marília, with an accessible pedagogical approach, demonstrates media literacy and commitment to teacher training, enriching activities.
Anthropological research on intangible cultural heritage: Local challenges and practices - introduction
Authors: Andjelkovic Biljana
Published: October 2024

The issue entitled Anthropological Research of Intangible Cultural Heritage:
Local Challenges and Practices contains seven papers in which the authors
consider various aspects of the intangible cultural heritage system that
researchers have noted in Serbia, Bosnia and Herzegovina, Montenegro and
Croatia. The papers highlight the administrative and organizational aspect
of intangible cultural heritage, as well as the problems of practical
research, documenting and registration of certain cultural elements in
national registers.
Towards an integrative approach to the safeguarding of living heritage: Programmes of the center for the intangible cultural heritage of Serbia and the Ethnographic Museum in Belgrade
Authors: Mladenovic-Ribic Natasa
Published: October 2024

The inscription of the element of living heritage in the National Registry of
Intangible Cultural Heritage, which is managed by the Center for the
Intangible Cultural Heritage of Serbia at the Ethnographic Museum in
Belgrade, can be observed as just one step in the work on safeguarding
social practices and skills, transmitted from generation to generation, that
have been maintained to this day and represent an important constituent of
identity of the communities and groups of practitioners. Although
safeguarding of living heritage depends primarily on the willingness of the
bearers to work on further recreation and transmission of knowledge and
skills, cooperation between those who inherit this heritage and custodian
institutions can contribute to raising awareness of the importance of
further practicing of ICH and increase in number of bearers. Starting from
the UNESCO concept of living heritage, I will systematically present
programmes of the Center for the Intangible Cultural Heritage and the
Ethnographic Museum, which are aimed at establishing and strengthening
cooperation with bearers, local communities, as well as other interested
parties in order to work together on presentation and education about the
elements of living heritage. Therewithal, I will analyze these safeguarding
programmes in the broader context of changes that have occurred in
contemporary museology, which include consideration and redefinition of the
role of museums in society, expansion of spectrum of museum activities, and
transformation of museum audiences from passive observers to active
participants and creators of content.
Intangible cultural heritage and the temptations of the neoliberal market
Authors: Matic Milos
Published: October 2024

The definition and, more broadly, understanding of the notion of the
intangible cultural heritage in the UNESCO Convention for the Safeguarding
of the Intangible Cultural Heritage is fundamentally laid down from the
positions of culture and is based on anthropological and, in general,
cultural understandings of heritage. Such an approach does not directly
involve the economic aspects of the intangible cultural heritage, even when
it relates to its safeguarding. The suppression of the economic aspect is
quite expected in the cultural paradigm, but in the world of the dominant
market economy, which shapes lifestyles in many societies, it seems like a
disregard for reality. The safeguarding of many elements of the intangible
heritage is directly correlated with the economic system of the community
that practice some intangible cultural heritage. Here, the basic principles
of safeguarding the intangible cultural heritage in given economic
conditions are problematized, i.e. safeguarding risk factors that come from
the economic sphere of life of the community that is the bearer of cultural
practices. The paper seeks to consider through an economic perspective the
collision of striving towards the preservation of cultural heritage and the
need of the community to survive in given economic and social circumstances.
It is based on research into the culture of economic behaviour and
intangible cultural heritage, and the basic approach is economic
anthropological.
Exploring Structural and Molecular Features of Sciatic Nerve Lesions in Diabetic Neuropathy: Unveiling Pathogenic Pathways and Targets
Authors: Schwarz Daniel , Le Marois Maxime , Sturm Volker , Peters Andreas S. , Longuespée Rémi , Helm Dominic , Schneider Martin , Eichmüller Bastian , Hidmark Asa S. , Fischer Manuel , Kender Zoltan , Schwab Constantin , Hausser Ingrid , Weis Joachim , Dihlmann Susanne , Böckler Dittmar , Bendszus Martin , Heiland Sabine , Herzig Stephan , Nawroth Peter P. , Szendroedi Julia , Fleming Thomas
Published: October 2024

Lesioned fascicles (LFs) in the sciatic nerves of individuals with diabetic neuropathy (DN) correlate with clinical symptom severity. This study aimed to characterize the structural and molecular composition of these lesions to better understand DN pathogenesis. Sciatic nerves from amputees with and without type 2 diabetes (T2D) were examined using ex vivo magnetic resonance neurography, in vitro imaging, and proteomic analysis. Lesions were only found in T2D donors and exhibited significant structural abnormalities, including axonal degeneration, demyelination, and impaired blood-nerve barrier (BNB). Although non-LFs from T2D donors showed activation of neuroprotective pathways, LFs lacked this response and instead displayed increased complement activation via the classical pathway. The detection of liver-derived acute-phase proteins suggests that BNB disruption facilitates harmful interorgan communication between the liver and nerves. These findings reveal key molecular mechanisms contributing to DN and highlight potential targets for therapeutic intervention.

Article Highlights
When does metabolic memory start? Insights from the AMD Annals Initiative on stringent HbA1c targets.
Authors: T. Russo Giuseppina , Nicolucci Antonio , Lucisano Giuseppe , Chiara Rossi Maria , Ceriello Antonio , Prattichizzo Francesco , Manicardi Valeria , Rocca Alberto , Di Bartolo6 Paolo , De Cosmo Salvatore , Di Cianni8 Graziano , Candido Riccardo
Published: October 2024

Abstract

Early, intensive glycemic control in T2D patients is associated with long-term benefits on cardiovascular disease (CVD) development. Evidence on benefits of achieving HbA1c targets close to normal values is scant. Subjects with newly-diagnosed T2D, without CVD at baseline, were identified in an Italian clinical registry (N=251,339). We adopted three definitions of early exposure periods (0–1, 0–2 and 0–3 years). Mean HbA1c was categorized into HbA1c < 5.7%, 5.7-6.4%, 6.5-7.0%, 7.1-8.0%, and >8.0%. The outcome was the incidence of major cardiovascular events. After a mean follow-up of 4.6±2.9 years, at multivariate Cox regression analysis, compared with mean HbA1c <5.7% during the first year after diagnosis, the increase in the risk of CVD was 24%, 42%, 49% and 56% for patients with HbA1c of 5.7%-6.4%, 6.5%-7.0%, 7.1%-8.0%, and >8.0%, respectively. The same trend was documented in all exposure periods. In conclusion, our data support that an early achievement of stringent targets of HbA1c <5.7% is worthy for CVD prevention.

Highlights · Why did we undertake this study?

The CVD impact of achieving very strict HbA1c targets soon after the T2D diagnosis is unknown. ·

What is the specific question(s) we wanted to answer?

Would near- normal mean HbA1c levels 1-3 years after T2D diagnosis reduce CVD risk? ·

What did we find?

Compared with mean HbA1c <5.7% during the first year after diagnosis, CVD risk the was 24%, 42%, 49% and 56% higher for patients with HbA1c of 5.7%-6.4%,or above. ·

What are the implications of our findings?

Early achievement of HbA1c <5.7% is associated with a lower incidence of CV events.
Tissue engineering of vascular vessels: the methods of cells and scaffold combining
Authors: Nasredinov A. S , Lavreshin A. V
Published: October 2024

Cell seeding is one of the most important stages in tissue engineering. Attempting to achieve fast, efficient and reliable result researchers in vascular tissue engineering use advantages of the tubular geometry of the grafts with conjunction of physical forces, such as pressure difference, centrifugal, electrostatic, magnetic forces and their combinations. This review describes the main trends and challenges in scaffold developing, main cellular types used for vascular tissue engineering and various methods for cell seeding, their advantages and drawbacks.
Cell technologies for muscle tissue restoration. Part I: myocardium
Authors: Korsakov I. N. , Zorin V. L. , Eremin I. I , Zorina A. I. , Kotenko K. V. , Kopnin P. B. , Pulin A. A.
Published: October 2024

This review represents ongoing clinical studies devoted to recovery of heart muscle using cell technologies, as well as analysis of applied populations of cells.
Differentiation of epitheliocytes of mesonephric and paramesonephric ducts in human embryogenesis
Authors: Zheglova M. Yu
Published: October 2024

The aim of the research was to give the morphofunctional characteristics of embryonic histogenesis of the female genital tract abortuses. We have studied 50 abortuses at the age of 4-12 weeks of gestation. The fixation of material and of the sample preparations were carried out according to the principles of quantitative histochemistry. According to the data obtained, paramesonefric ducts are found in the composition of the urogenital blastema to which coelomic epithelium cells can migrate. Since in this time the more differentiated ducts are mesonefric and archinephron is the organ that carries out its function, it is undeniable that the development of paramesonefric ducts goes on vector of the cranio-caudal growth of mesonefric ducts. The formation of a common utero-vaginal canal occurs through the convergence of the paramesonefric duct walls. Herewith the septum between ducts is persisted. Epitheliocytes in the medial walls of the ducts are deformed, nuclei decrease in size. The shape of the nuclei changes and rectangular nuclei dominate. The cells which have a light nucleoplasm are detected, in other cells there is a piknotic nucleus in the center; there are cells with nuclei having the semilunar shape, cells with two fragments of the nucleus, one of which is dark and the other one is light; nucleus with cuspidal protrusions at one pole, cigar-shaped nucleus. The formation of the complex relationships between the paramesonefric ducts at the entrance into mesenchymal block allows to make a suggestion on the critical period of the female genital tract development.
EFFECT OF CURCUMIN AND GLIOTOXIN ON FIBROBLASTS FROM THE LARGE PORTAL TRACTS OF RATTUS NORVEGICUS
Authors: Mijanovic O. , Zhuravleva M. N , Gomzikova M. O , Rizvanov A. A , Kiassov A. P
Published: October 2024

In recent years there were lots of researches focused on inhibiting connective tissue cells in the liver of patients with chronic hepatitis. Most studies of the substances which can potentially be considered as drugs for the treatment of liver fibrosis were performed on hepatic stellate cells. However, there is a theory of portal fibroblasts participation in liver fibrosis, as in most cases the processes of fibrogenesis start both at main portal tract and intrahepatic ducts. Suppression of fibrogenesis by preventing the activation and induction of apoptosis of portal fibroblasts are being considered as therapeutic strategies for the treatment of liver fibrosis.
Effects of platelet factor 4 on morphological and biochemical signs of apoptosis in T-lymphocytes
Authors: Skibo Y. V , Fathullina A. R , Romanova E. V , Litvinov R. I
Published: October 2024

Platelet factor 4 (PF4) pertains to a family of CXC chemokines released by activated platelets. PF4 has a broad spectrum of effects on different cell types, including modulation of the immune response. In this study, we explore effects of PF4 on the morphological and biochemical signs of apoptosis in human T-lymphocytes in vitro. T-lymphocytes were isolated from peripheral blood of healthy donors using negative immunomagnetic separation and cultured in the complete RPMI 1640 medium for 24 hours in the absence and presence of PF4 added at a final concentration of 2 /ug/ml or 100 ug/ml. After 2, 4, 6, 12 and 24 hours of incubation the cells were studied with transmission electron microscopy and Western blot analysis with respect to potential apoptotic changes. The electron microscopy of control T-lymphocytes showed that the vast majority of the cells had a morphology characteristic of apoptosis at different stages. Adding PF4 at a concentration of 2 ug/ml reduced the number of cells at the late stages of apoptosis, while maintaining the signs of the early apoptosis in most of the T-lymphocytes. In the presence of 100 ug/ml PF4 nearly all of the cells kept a typical morphology of normal T-cells throughout the time of cultivation. The morphological apoptotic changes correlated well with expression of caspase-3, which was clearly detected in the control cells and cells treated with 2 ug/ ml PF4, but was almost abolished in the cells treated with 100 ug/ml PF4. Our results provide direct evidence for the dose-dependent anti-apoptotic effects of PF4 on T-cells, suggesting that PF4 sustains an immune response by extending T-lymphocyte survival.
Hepatic stellate cells stimulate liver regeneration after partial hepatectomy under inhibition of hepatocyte proliferation
Authors: Titova A. A , Burganova G. R , Sharipova E. I , Pevnev G. O , Mavlikeev M. O , Gazizov I. M , Galyavieva A. R , Shafigullina A. K , Kaligin M. S , Titova MA. A , Gumerova AA. A , Kiassov A. P
Published: October 2024

Most of the fundamental studies in the field of developing new methods for treating liver diseases in regenerative medicine are performed with haematopoietic and mesenchymal stem cells. At the same time more and more importance is gaining need for finding new approaches enabling stimulation of regional stem cell compartment and using regional stem cells which are possibly represented by hepatic stellate cells. But studies aimed at their transplantation are rare. Previously, we have established the possibility of these cells to differentiate into hepatocytes after transplantation to rats with partial hepatectomy. In our present research we studied liver regeneration after transplantation of hepatic stellate cells on partial hepatectomy with 2-acetylaminofluorene damage model in rats. 2-acetylaminofluorene blocks hepatocyte proliferation. Results of study confirmed that hepatic stellate cells have the ability to differentiate into hepatocyte and stimulate it's regeneration without the threat of fibrosis after transplantation in rats with partial hepatectomy and administration of 2-acetylaminofluorene.
Hematopoietic stem cells with induced apoptosis effectively inhibit glioma cell growth in vitro, but started new mechanism of tumor stem cells
Authors: Bryukhovetskiy I. S , Mischenko P. V , Tolok E. V , Khotimchenko Yu. S. , Zaitsev S. V , Bryukhovetskiy A. S
Published: October 2024

The presence of hematopoietic stem cells in culture inhibits proliferation of tumor cells. We hypothesized that pretreatment of stem cell apoptosis inducers increase their anti-tumor efficacy. The aim of the study was to explore the possibility of stem cells to induce apoptosis effectively inhibit the growth and induce the death of tumor cells in vitro. Use line C6 glioma cultures and hematopoietic (CD34+/CD45 + ) stem cells. We used dexamethasone as the apoptosis inducer. It's known that hematopoietic stem cells with apoptosis induced more effectively inhibit growth and induce a death of C6 glioma cells than native stem cells. In the process of intercellular interaction we observed phenomenon of merging stem and tumor cells, which apparently is one of the mechanisms of new tumor stem cells formation.
Effects of Dalarginum on proliferation of multipotent mesenchymal stromal cells, dermal fibroblasts, and human osteosarcoma cells in vitro
Authors: Vasilyev A. V , Bukharova T. B , Volkov A. V , Vikhrova E. B , Bolshakova G. B , Goldstein D. V
Published: October 2024

The aim of this work was to assess in vitro the effect of various doses of Dalarginum and its blocker Naloxone on the proliferation of multipotent mesenchymal stromal cells (MMSC), dermal fibroblasts (FD) and human osteosarcoma cell (line HOS). We used MMSC and FD derived from three donors. The cells were cultured for 3 days with solutions of the test substances at following concentrations: Dalarginum 10 jg/l, 100 jg/l, 1000jg/l; combination of naloxone 0.5 mg/l and Dalarginum, 100 jg/l; combination of Naloxone 3 mg/l and Dalarginum, 100jg/l. Saline was added in the culture medium in the control group. The cells were counted by the end of day 3 of culturing. Dalarginum at 100 jg/l significantly increased the number of MMSC by 19-34 % and decreased the count of cells (line HOS) by 22-34 % compared with the control values. There were no significant differences in cell numbers between the groups with addition of dalarginum at 10jg/l and 1000jg/l as well as significant changes in the number of FD under the influence of the test substances. Dalarginum combined with Naloxone, opioid receptor antagonist, had no impact on the number of cells, which confirmed its receptor-mediated action. The optimum effect of Dalarginum on opioid receptors was observed at100jg/l.
Gastrointestinal stromal tumor cell lines - the mechanisms of chemosensitivity in vitro
Authors: Boichuk S. V , Ramazanov B. R , Galembikova A. R , Mustafin I. G , Duensing A.
Published: October 2024

This research was aimed to study the sensitivity of gastrointestinal stromal tumors (GISTs) to chemotherapeutic drugs. We studied the chemosensitivity of GISTs by using several GIST cell lines. Cytotoxicity of chemotherapeutic drugs was assessed by colorometric MTS-assay. The expression level of DNA damage markers and repair proteins was assessed by immunoblotting. Apoptosis analysis was conducted by measuring the amount of hypodiploid cells by using a propidium iodide DNA staining procedure and flow cytometry analysis. We found that GISTs are sensitive to certain chemotherapeutic drugs - topoisomerase II inhibitors (etoposide and doxorubicine). DNA damage induction (in particular, DNA double-strand breaks formation) in GISTs induced cell apoptotic cell death despite the activation of DNA repair pathways. Of note, this effect was observed in imatinib-sensitive and resistant GIST cell lines, as well. We also found an increase accumulation phosphorylated form of histone 2A in GISTs after imatinib treatment. This effect was associated with the significant reduction of Rad51 recombinase expression, known as a key factor of homologous recombination. Taken together, this might explain the possible mechanisms of cell death in GISTs after targeted therapy. We found the chemosensitivity of GIST cell lines to some certain chemotherapeutic agents. Thus, the current point of view indicating about GISTs resistance to chemotherapy is needed to be re-evaluated.
Cancer stem cells: 20 years behind
Authors: Mingaleeva R. N , Miftakhova R. R , Rizvanov A. A
Published: October 2024

The biology of cancer stem cells (CSC) has been investigated and debated for past 20 years. Owning to their exceptional plasticity and the complexity of controlled processes CSC inspire the development of new research techniques for cell isolation and targeted therapy. This review is an attempt to summarize recent data and trends in the field of cancer stem cells and discuss potential and weaknesses of new research techniques
Genetic and epigenetic mechanisms of sensory maps development
Authors: Mitrukhina O. , Minlebaev M. , Khazipov R.
Published: October 2024

One of the central questions of neurobiology is to understand how during development billions of neurons establish synaptic connections, and what are the roles of genes and activity in the formation of specific neuronal circuits . There are two main theoretical models to describe this developmental process . The model of “tabula rasa” implies that initially there is no order in synaptic connections, and that their initial exuberant number undergoes pruning through the competition of neurons for their targets . Alternative model assumes that development of synaptic connections is strictly determined by genes, through signaling molecules that predefine highly ordered connectivity, and that the activity only plays confirmative roles . In the present review, we describe genetic and epigenetic factors involved in the development of sensory maps in barrel cortex and provide evidence that both mechanisms operate in the development of this system . While genetic mechanisms provide course topography of somatosensory map at subcolumnar precision level in its initial state, sensory-driven activity patterns, which are is expressed in barrel cortex during the critical developmental period support competition between sensory inputs (neighbor whiskers) for the cortical territories to achieve columnar level of precision in somatosensory map by the end of the critical period
Targeted radionuclide therapy: current status and prospects
Authors: Vodeneev V. A , Zvyagin A. V , Shilyagina N. Yu , Kulikov D. A , Kulikov A. V , Gudkov S. V
Published: October 2024

One of the intensively developing approaches to the treatment of oncologic diseases is directed (targeted) radionuclide therapy. Radionuclide therapy avoids the side effects associated with external beam therapy. Furthermore, it is possible to combine the processes instrumental diagnostics and radiotherapy (theranostics), which leads to personalize the treatment regimen for each individual patient. in this review, we discuss the fundamentals of targeted radionuclide therapy, including the characteristics of the radionuclides and biomolecular targeting moieties information on the targeted radionuclide therapy drugs for approved for clinical use is provided. Prospects and limitations of the targeted radionuclide therapy and their implementation in clinical practice are discussed
Effect of gelatin cryogel on proliferation and synthetic activity of fibroblasts in excision wound model
Authors: Yergeshov A. A , Siraeva Z. Y , Kazakova R. R , Mullin R. I , Davliev D. M , Zakirova A. A , Salikhova T. I , Kuznetsova E. V , Luong D. T , Savina I. N , Abdullin T. I
Published: October 2024

We prepared an experimental sample of gelatin based cryogel membrane with pore size of 50-150 μm. Confocal microscopy and LDH assay showed that the cryogel macroporous structure promotes migration and proliferation of human skin fibroblasts within the matrix in vitro. To assess in vivo effect of the cryogel an excision wound model in rats was tested The cryogel significantly increases the number of fibroblasts as well as the density and order of produced collagen fibers in the dermis to day 7 of the wound healing process. The results suggest the stimulating effect of the gelatin cryogel on fibroblasts activity and demonstrate its potential for skin regeneration
Proliferation and transdifferentiation of endocrinocytes of the rat during experimental diabetes
Authors: Kaligin M. S , Mavlikeev M. O , Titova A. A , Plushkina A. S , Titova M. A , Gumerova A. A , Kiassov A. P
Published: October 2024

Some studies have found an increase number of α-cells in experimental diabetes, which may cause rising of blood glucose levels, along with the lack of insulin. But the mechanism of increasing the amount of glucagon-positive cells is still unknown. The aim of the study was to investigate the proliferative activity and the possibility of differentiation of α- and β-cells of the islets of Langerhans of pancreas during experimental diabetes in rats The work was performed on 33 white mongrel male rats. After alloxan injection, blood glucose levels were measured by glucose oxidase method and the expression of insulin, glucagon, and proliferating cell nuclear antigen was studied. Isolated proliferating glucagon-positive cells were found only on day 14 of the experiment. At the same time of the experiment bigormonal cells were found that synthesize insulin and glucagon. The results of the double staining for PCNA and glucagon showed that the increasing number of glucagon-positive cells in early stages of experimental diabetes is not related to their proliferation Probably it is due to differentiation of the progenitor cells of the islets in pancreas
The role of reactive oxygen species and nitric oxide in the realization of the adaptation mechanisms of bone-derived multipotent mesenchymal stromal cells to hypoxia under cultivation with growth factor bFGF
Authors: Poleshko A. G , Volotovski I. D
Published: October 2024

The simulation of conditions in vivo under cultivation the stem cells in vitro as well gas media content (low O2 concentrate) and different growth factors presence is one of the feasible way of growth rate of multipotent mesenchymal stromal cells (MMSC) culture increase when kept differentiation potential and maintained high viability. Oxygen metabolites: reactive oxygen (ROS) and nitrogen species are the most interesting for studying the influence of above characteristics on proliferation activity and viability of MMSC So it is currently important to identify ROS and NO role in MMSC by cooperative cultivation with growth factor bFGF under hypoxia. The research was aimed to study an effect of 5% hypoxia on Н2О2, О2- and NO content in MMSC from bone marrow in the cell culture in the presence or absence bFGF It was found out that both 5% hypoxia and bFGF (7 ng/ml) decrease the intracellular Н2О2, О2- and NO concentration on the background of elevated HIF1α gene expression and depressed p53 gene expression. At the same time simultaneous action of hypoxia and bFGF promotes minimal ROS and NO generation, maximizes the effects on HIF1α and p53 genes expression probable for the genome protection Taking into account the increasing the proliferative activity and viability of MMSC culture under these conditions that was shown previously by us, obtained results indicate the regulatory role of ROS and NO in the long-term MMSC adaptation to 5% hypoxia, simulating O2 physiologic content as in vivo
Different expression of hematopoietic-supporting genes in cord, placental and bone marrow mesenchymal stromal cells
Authors: Kostjunina V. S , Petyovka N. V , Potapnev M. P
Published: October 2024

Human multipotent mesenchymal stromal cells (MMSC) from bone marrow (BM), umbilical cord (UC) and chorion villi (CV) were isolated and cultured in xeno-free media supplemented with AB human serum There were no differences in expression of CD31, CD33, CD34, CD45, CD90, СD105, CD117, HLA-ABC, HLA-DR between BM, UC and CV MMSC Human AB serum (5%) accelerated proliferation of UC MMSC in vitro. Expression of genes opn, scf, cxcl12, il-3, il-6, il-8, il-11, g-csf, gm-csf, epo, and nes was studied in Real-Time PCR. Up-regulation the expression gene nes in CV MMSC and genes g-csf and il-11 (but 6-fold down-regulation of cxcl12) in UC MMSC, was revealed when compared to BM MMSC (p
Regeneration of rat sciatic nerve using tubulation and allogeneic transplantation of adipose-derived mesenchymal stromal cells
Authors: Masgutov R. F , Masgutova G. A , Rogojin A. A , Zhuravleva M. N , Zakirova E. Y , Nigmetzyanova M. V , Mukhametova L. R , Shulman A. A , Mukhamedshina Y. O , Yafarova G. G , Rizvanov A. A
Published: October 2024

The effectiveness of application of biodegradable conduit (NeuraGen; INTEGRA, USA) in combination with adipose derived multipotent mesenchymal stem cells and fibrin sealant (Tissucol-Kit; Baxter AG, Austria) on posttraumatic peripheral nerve regeneration was investigated using a rat model of sciatic nerve injury. We demonstrate that our proposed cell therapy confers a neuroprotective effect under our experimental design
A morphological investigation of the polyhydroxybutyrate/valerate and polycaprolactone biodegradable small-diameter vascular graft biocompatibility
Authors: Antonova L. V , Mukhamadiyarov R. A , Mironov A. V , Burago A. Yu , Velikanova E. A , Sidorova O. D , Kudryavtseva Yu. A , Barbarash O. L , Barbarash L. S
Published: October 2024

We studied the integration of polyhydroxybutyrate/ valerate (PHBV)/polycaprolactone (PCL) biodegradable electrospun vascular grafts with organism cells and tissues Grafts were implanted into abdominal aorta of Wistar rats for twelve months. All the grafts were patent during the whole period of implantation. Twelve months postimplantation, 25% of the grafts were intact, and 25% of the grafts had parietal thrombus only at anasthomotic sites and thrombusfree surface at all other sites Regarding other grafts, we revealed minor connective tissue hyperplasia in 41 7% of the grafts, and an inflammatory infiltrate in the part of the arterial wall in 8. 3% of the grafts. Using original technique of the morphological investigation, we found that polymer degradation was accompanied by the replacement of the graft wall by various cells (macrophages, fibroblast-like cells, epithelioid cells, and multinucleated giant cells) with the formation of extracellular matrix instead of the polymer scaffold Therefore, long-term patency and the results of the morphological study confirmed the possibility to use PHBV/PCL tubular polymer construct in the creation of small-diameter vascular grafts
Influence of allogeneic hematopoietic stem/ progenitor cells treated with IL-3 on structural-functional heart changes under experimental postinfarction heart failure in long-term period of observation
Authors: Severova E. A , Berkinbayev S. F , Nugmanova M. N , Pominova N. M , Perfilyeva Yu. V , Supniyazova T. A , Denisov Yu. D , Belyaev N. N
Published: October 2024

The aim of this research was to study the influence of allogeneic hematopoietic stem/ progenitor cells infused intravenously to rats in acute period of izoproterenol-induced myocardial infarction on structural and functional changes of the heart in long-term period of observation (over 1 year) The postinfarction heart failure was created by intra-abdominal injection of isoproterenol solution Allogenic CD117+ hematopoietic stem/ progenitor cells were extracted from bone marrow by immunomagnetic separation and were induced for CXCR4 expression and TGFb production by cultivation for three days in presence of interleukine-3. Obtained cells were injected intravenously to rats on 9 day after isoproterenol administration. One year later structure and function of the myocardium were investigated by epicardial echocardiography in M-modal mode with the apparatus «VIVID-3» supplied by 3. 5 MHz transduser The diastolic function was examined by Doppler echocardiography method in the impulse mode One year later, in the group of infarcted animals with hematopoietic stem/ progenitor stem cell injection the clinically and statistically significant reduction of the left ventricle weight and relative thickness of the left ventricle wall were revealed; furthermore - the reduction of the left ventricle back wall thickness, interventricular septum thickness and aorta diameter were observed in comparison with the group without treatment Doppler echocardiographic test has demonstrated an improvement of cardiac diastolic function in the group of infarcted animals with hematopoietic stem/ progenitor cell injection: statistically significant reduction of proportion of the animals with hypertrophic and restrictive mode of transmitral blood flow Obtained data have shown that single intravenous injection of hematopoietic stem/ progenitor cells treated with IL-3 ex vivo reduces a risk of serious cardiovascular complications after myocardial infarction at long-term period of observation in rats Proposed approach may be useful in improving of cell therapy effectiveness for heart failure treatment
Soluble and immobilized papain and trypsin as destroyers of bacterial biofilms
Authors: Trizna E. Yu , Baydamshina D. R , Kholyavka M. G , Sharafutdinov I. S , Hairutdinova A. R , Khafizova F. A , Zakirova E. Yu , Hafizov R. G , Bogachev M. I , Kayumov A. R
Published: October 2024

The proteolytic enzymes are widely used in medicine as a wound healing agents, removing necrotic tissues and serving as an alternative to surgery. The ability of soluble and immobilized papain and trypsin to destroy bacterial biofilm was investigated. We show that treatment with papain leads to disruption of biofilms formed by Pseudomonas aerugenosa, Escherichia coli, Micrococcus luteus, and in a lesser extent of Staphylococcus aureus and Staphylococcus epidermidis. It is shown that none of the investigated enzymes has mutagenicity and cytotoxicity, and causes no increase in the amount of necrotic cells in culture in vitro
Expression of myogenesis genes by gingiva derived cells
Authors: Samchuk D. P , Lukyanova E. N , Eremin I. I , Zorin V. L , Zorina A. I , Grinakovskaya O. S , Korsakov I. N , Deev R. V , Gilmutdinova I. R , Lazareva N. L , Eremin P. S , Petrikina A. P , Gomzyakov A. E , Timashkov D. A , Vit’ko N. K , Kotenko K. V , Kopnin P. B , Pulin A. A
Published: October 2024

The ability of gingiva derived mesenchymal stromal cells (MSCs) to myogenic differentiation and the possibility of their expansion in vitro revealed in our previous work, open up prospects for their use in regenerative medicine for the correction of muscle pathology. Data on changes in the expression of myogenic regulatory factors in gingiva derived MSCs can serve as confirmation of the possibility of differentiation of these cells into the myogenic direction The study was conducted on cultures of gingiva derived MSCs and skin fibroblasts. When comparing gene expression profiles of gingiva derived MSCs and skin fibroblasts significant changes have been registered for 153 genes. Of these, we selected 19 significant differentially expressed genes, for which main signal pathways were analyzed. Evidences of significant activation / inactivation of signaling pathways and key genes that have been identified for the population of human satellite cells were obtained during analysis of the genome transcriptome profiles of gingiva derived MSCs This applies to such signaling pathways as the p38 MAPK, NOTCH and other groups responsible for the proliferation and differentiation in myogenic direction, the processes of self-renewal or self-maintenance in the population of satellite cells. At the same time full match in the patterns of gene expression between the stable population of gingiva derived MSCs and human satellite cells undergoing myogenic differentiation as well as cells derived from atypical sources and differentiated in the myogenic direction was not observed The data of our experiment suggests that the gingiva derived MSCs are distinct from satellite cells and could be considered as population with plateaued development, possessing the ability to differentiate into myogenic direction due to non-canonical expression pattern of myogenic regulatory factors
Polymorphism of genes of the antioxidant system in patients with erysipelas and their role in development of the disease
Authors: Emene Ch. P , Kravchenko I. E , Aibatova G. I , Rizvanov A. A
Published: October 2024

In this research we analyzed the genotype distribution of polymorphisms of genes involved in antioxidant system of erysipelas patients and healthy population in Republic of Tatarstan, Russia. On comparing genotype distribution with healthy controls, we identified that SNPs of superoxide dismutase 1 - SOD1g7958a, superoxide dismutase 2 - SOD2 T5482C (Ala16Val) and catalase - CAT C262T affected the predisposition to erysipelas infection and clinical features
Efficiency of autofibroblasts in surgical treatment of parodontitis
Authors: Grudyanov A. I , Zorin V. L , Pereverzev R. V , Zorina A. I , Bozo I. Y
Published: October 2024

The destructive forms of periodontal disease have a high prevalence. Our study was aimed to evaluate the effectiveness of tissue-engineering osteoplastic material in treatment of patients with periodontal pathology. We have obtained autologous gingival fibroblasts of patients, combined with the hydroxyapatite carriage and transplanted on a standard surgical treatment of periodontitis destructive forms. As a control we used the material without cells implanted into periodontal defects of other affected teeth of the same patients. We have shown the better effect of tissue-engineering osteoplastic material regarding the periodontal pockets depth, but significant difference in bone regeneration and other indicators has not been revealed. Thus, a continuation of researches is required to modify the technology of tissueengineering osteoplastic material creation and to choice the more optimal carrier for gingival fibroblasts.
Construction and biological effect evaluation of gene-activated osteoplastic material with human vegf gene
Authors: Deev R. V , Drobyshev A. Yu , Bozo I. Ya , Galetskiy D. V , Korolev V. O , Eremin I. I , Filonenko E. S , Kiselev S. L , Isaev A. A
Published: October 2024

Development of new effective osteoplastic materials is highly requested in practice of traumatology and orthopedics, oral and maxillofacial surgery. The goals of our research were design and construction of gene-activated bone graft (GABG) consisting of collagen/hydroxyapatite scaffold and plasmid DNA encoding vegf-a165 and evaluation of its biological effect in vitro and in vivo. We have shown that GABG co-incubation with multipotent mesenchymal stromal cells increased their VEGF protein expression. After GABG implantation into parietal bones defects the transfection of «recipient bed cells» was observed and accompanied by more pronounced angiogenesis as compared with control. The lager volume of bone regenerate was in case of GABG on 15 and 30 days after application. The source of reparative osteogenesis was not only parietal bones but also the GABG fragments (even from central part of the defect) majority of which were surrounded by newly formed bone tissue. In control group no osteoinductive effect has been observed. Thus, GABG with plasmid DNA encoding VEGF-A165 possesses angiogenic activity providing osteoinductive properties.
Plant and animal stem cells: two sides of the same medal
Authors: Zubov D. A
Published: October 2024

The review offers up-to-date analysis on plant stem cell biology, meristems functioning, including plant regeneration mechanisms and tumorigenesis, as well as biotechnology methods of plant tissue culture. There were also compared the plant and animal stem cells, their probable polyphyletic origin in aspect of divergent evolution of modular and unitary organisms in both plant and animal kingdoms.
Adipose-derived stromal vascular fraction as an alternative source of cells for the regenerative medicine
Authors: Veremeev Ä. V , Bolgarin R. N , Petkova M. A , Katz N. , Nesterenko V. G
Published: October 2024

The adipose tissue is considered as the most convenient and abundant source of cells for the regenerative medicine. The number of progenitor cells in the adipose tissue significantly exceeds their amount in the bone marrow and other tissues. Therefore, adipose-derived stromal vascular fraction comprising distinct populations of stem and progenitor cells can be relatively easily isolated from lipoaspirates and may then be used in various pathological conditions. However, the profile of this cell fraction with a significant therapeutic potential remains unclear, and there are no standardized protocols for its isolation and evaluation. in this article, we reviewed the data on the potential use of adipose-derived stromal vascular fraction in the regenerative medicine. We described the main historical milestones and performed a comprehensive analysis of the sources of adipose-derived stromal vascular fraction, techniques of its isolation, features, immunophenotype and differentiation pathways