bluuebunny/crossref_metadata_2025 huggingface dataset:
Field Observation and Settlement Prediction Study of a Soft Soil Embankment under Rolling Dynamic Compaction
Authors: Chen Dashuo , Wu Yuedong , Liu Jian , Wu Huiguo , Ren Yuzhe
Published: July 2024

Rolling dynamic compaction (RDC) has been found to be useful for compaction soils and is now widely used globally. Because RDC is not often used in soft soils with poor engineering properties, field monitoring was used to study the soft clay embankment responses under RDC conditions in this study. Analysis of the monitoring data revealed that the response of the soil occurred mainly in the first 20 passes. Field monitoring revealed a strong correlation between settlement, horizontal displacement, and pore water pressure. The depth of impact of RDC on the soft soil embankment was between 3 and 3.5 m. Although settlement prediction is an important issue for construction, there is a lack of prediction methods for RDC-induced soil settlement. In this study, we used three different machine learning algorithms: random forest regression (RFR), multilayer perceptron (MLP), and extreme gradient boosting (XGBoost) to predict the total settlement and uneven settlement induced by RDC on the soft soil embankment. The three prediction models were compared, and the predictive accuracy of these models was assessed. This study analyzes and summarizes the effect of RDC application on a soft clay embankment and explores the machine learning method used for settlement prediction based on monitoring data, which provides some methods and ideas for research on the application of RDC on soft soil foundations.
Monitoring the Sleep Respiratory Rate with Low-Cost Microcontroller Wi-Fi in a Controlled Environment
Authors: Burimas Ratthamontree , Horanont Teerayut , Thapa Aakash , Lamichhane Badri Raj
Published: July 2024

Sleep apnea, characterized by breathing interruptions or slow breathing at night, can cause various health issues. Detecting respiratory rate (RR) using Wireless Fidelity (Wi-Fi) can identify sleep disorders without physical contact avoiding sleep disruption. However, traditional methods using Network Interface Cards (NICs) like the Intel Wi-Fi Link 5300 NIC are often costly and limited in channel state information (CSI) resolution. Our study introduces an effective strategy using the affordable ESP32 single-board computer for tracking RR through detailed analysis of Wi-Fi signal CSI. We developed a technique correlating Wi-Fi signal fluctuations with RR, employing signal processing methods—Hampel Filtering, Gaussian Filtering, Linear Interpolation, and Butterworth Low Pass Filtering—to accurately extract relevant signals. Additionally, noise from external movements is mitigated using a Z-Score for anomaly detection approach. We also implemented a local peak function to count peaks within an interval, scaling it to bpm for RR identification. RR measurements were conducted at different rates—Normal (12–16 bpm), Fast (>16 bpm), and Slow (<12 bpm)—to assess the effectiveness in both normal and sleep apnea conditions. Tested on data from 8 participants with distinct body types and genders, our approach demonstrated accuracy by comparing modeled sleep RR against actual RR measurements from the Vernier Respiration Monitor Belt. Optimal parameter settings yielded an overall average mean absolute deviation (MAD) of 2.60 bpm, providing the best result for normal breathing (MAD = 1.38). Different optimal settings were required for fast (MAD = 1.81) and slow breathing (MAD = 2.98). The results indicate that our method effectively detects RR using a low-cost approach under different parameter settings.
Large-Depth Ground-Penetrating Radar for Investigating Active Faults: The Case of the 2017 Casamicciola Fault System, Ischia Island (Italy)
Authors: Paoletti Valeria , D’Antonio Donato , De Natale Giuseppe , Troise Claudia , Nappi Rosa
Published: July 2024

We conducted large-depth Ground-Penetrating Radar investigations of the seismogenic Casamicciola fault system at the volcanic island of Ischia, with the aim of constraining the source characteristics of this active and capable fault system. On 21 August 2017, a shallow (hypocentral depth of 1.2 km), moderate (Md = 4.0) earthquake hit the island, causing severe damage and two fatalities. This was the first damaging earthquake recorded on the volcanic island of Ischia from the beginning of the instrumental era. Our survey was performed using the Loza low-frequency (15–25 MHz) GPR system calibrated by TDEM results. The data highlighted variations in the electromagnetic signal due to the presence of contacts, i.e., faults down to a depth larger than 100 m below the surface. These signal variations match with the position of the synthetic and antithetic active fault system bordering the Casamicciola Holocene graben. Our study highlights the importance of employing large-depth Ground-Penetrating Radar geophysical techniques for investigating active fault systems not only in their shallower parts, but also down to a few hundred meters’ depth, providing a contribution to the knowledge of seismic hazard studies on the island of Ischia and elsewhere.
Requirements Engineering for a Drone-Enabled Integrated Humanitarian Logistics Platform
Authors: Aretoulaki Eleni , Ponis Stavros T. , Plakas George
Published: July 2024

The pursuit of ameliorating humanitarian logistics (HL) through the integration of cutting-edge technologies has received significant attention in recent years. AIRDROP is a visionary platform conceived to offer a cohesive disaster management approach spanning from preparedness to recovery of a wide range of natural and human-made disasters. AIRDROP aims to be a scalable, modular and flexible solution, employing an array of drones of different sizes and payload capabilities, able to provide different HL services to first responders and operational decision-makers. This study aims to elicit, specify and validate the requirements for AIRDROP to ensure their applicability across a broad spectrum of disaster scenarios and the entire disaster management continuum. This research utilized a thorough literature review and expert consultations to systematically elicit and specify the AIRDROP requirements, ensuring they were grounded in both academic foundations and practical industry standards. The validation process involved a questionnaire survey administered to 26 participants from various professional backgrounds. The requirements were prioritized using the MoSCoW methodology, and significant differences among participant groups were identified through the Kruskal–Wallis H and Mann–Whitney U tests. Furthermore, two critical requirements emerged from open-ended responses. As a result, 276 out of the initially defined 335 requirements in total advanced to the design phase. It is worth noting that the dynamic nature of requirements in HL necessitates ongoing assessment and adaptation to keep AIRDROP at the forefront and aligned with evolving needs.
Strength Analysis of High-Pressure SCR System Based on Thermo-Fluid-Solid Coupling
Authors: Zhu Yuanqing , Yu Jia , Zhang Jin , Shi Jie , Wan Qiqi , Xia Chong
Published: July 2024

In the operation of a high-pressure selective catalytic reduction (HP-SCR) system, variations in the internal exhaust gas flow speed result in non-uniform pressure and temperature distribution within the reactor. These fluctuations, which are neither constant nor linear, can affect the safe and reliable operation of the high-pressure selective catalytic reduction (HP-SCR) system, so the strength simulation analysis is necessary. Based on the high-pressure selective catalytic reduction system of a thermo-fluid-solid coupling marine diesel engine as the research object, this study constructs a calculation model using Space Claim and utilizes computational fluid dynamics (CFD) and computer-aided engineering (CAE) numerical simulation methods to analyze the strength of the high-pressure selective catalytic reduction (HP-SCR) reactor. The results show that the overall pressure drop of the selective catalytic reduction system is 5500 Pa, and the overall temperature rise of the reactor is 24 °C, which mainly occurs in the first layer catalyst, accounting for 62.5%. The pressure and temperature load of the reactor change along the axial direction, and the axial deformation gradient of the cylinder is more. The maximum deformation of the reactor under thermal load is 15 times that under mechanical load, and 97% of the deformation is axial.
Air Quality Monitoring with Low-Cost Sensors: A Record of the Increase of PM2.5 during Christmas and New Year’s Eve Celebrations in the City of Queretaro, Mexico
Authors: Rodríguez-Trejo Alejandro , Böhnel Harald N. , Ibarra-Ortega Héctor E. , Salcedo Dara , González-Guzmán Reneé , Castañeda-Miranda Ana G. , Sánchez-Ramos Luis E. , Chaparro Mauro A. E. , Chaparro Marcos A. E.
Published: July 2024

The high concentrations of particulate matter (PM) in the atmosphere have adverse effects on both the environment and human health, as well as on urban and faunal biodiversity. Short-term events, such as the burning of fireworks, attributable to human activity, result in a significant and rapid increase in PM concentration levels within the atmosphere. We present findings derived from observations made using low-cost PM sensors deployed as part of the Environmental Monitoring Network in Querétaro, Mexico. These sensors utilize the Environmental Geo Monitoring Stations (EMGA) device, developed by the Institute of Geosciences at UNAM. The observation period spanned from 10 December 2023 to 10 January 2024, encompassed the Christmas and New Year holidays, and focused on PM2.5 concentrations, and a comparison with a similar period from 10 February 2024 to 10 March 2024 considered a typical activity period in the area. The results reveal two substantial increases in PM2.5 concentrations (from less than 50 µg/m3 to more than 340 µg/m3) over time and spatial distribution during this period, with notable increases observed during the festive season coincident with the Christmas and New Year celebrations. Specifically, prolonged periods exceeding up to 6 h were noted on festivity days. This research offers insight into the effects, trends, and spatial–temporal distribution of pollutants within the city of Queretaro, which may be used as a reference for other cities around the world.
Characteristics and Driving Mechanisms of Coastal Wind Speed during the Typhoon Season: A Case Study of Typhoon Lekima
Authors: Wang Lingzi , Fu Aodi , Bashir Bashar , Gu Jinjun , Sheng Haibo , Deng Liyuan , Deng Weisi , Alsafadi Karam
Published: July 2024

The development and utilization of wind energy is of great significance to the sustainable development of China’s economy and the realization of the “dual carbon” goal. Under typhoon conditions, the randomness and volatility of wind speed significantly impact the energy efficiency and design of wind turbines. This paper analyzed the changes in wind speed and direction using the BFAST method and Hurst index based on data collected at 10 m, 30 m, 50 m, and 70 m heights from a wind power tower in Yancheng, Jiangsu Province. Furthermore, the paper examined the causes of wind speed and direction changes using wind speed near the typhoon center, distance from the typhoon center to the wind tower, topographic data, and mesoscale system wind direction data. The conclusions drawn are as follows: (i) Using the BEAST method, change points were identified at 10 m, 30 m, 50 m, and 70 m heights, with 5, 5, 6, and 6 change points respectively. The change points at 10 m, 30 m, and 50 m occurred around node 325, while the change time at 70 m was inconsistent with other heights. Hurst index results indicated stronger inconsistency at 70 m altitude compared to other altitudes. (ii) By analyzing the wind direction sequence at 10 m, 30 m, 50 m, and 70 m, it was found that the wind direction changes follow the sequence Southeast (SE)—East (E)—Southeast (SE)—Southwest (SW)—West (W)—Northwest (NW). Notably, the trend of wind direction at 70 m significantly differed from other altitudes during the wind speed strengthening and weakening stages. (iii) Wind speed at 10 m and 70 m altitudes responded differently to the distance from the typhoon center and the wind near the typhoon center. The correlation between wind speed and the distance to the typhoon center was stronger at 10 m than at 70 m. The surface type and the mesoscale system’s wind direction also influenced the wind speed and direction. This study provides methods and theoretical support for analyzing short-term wind speed changes during typhoons, offering reliable support for selecting wind power forecast indicators and designing wind turbines under extreme gale weather conditions.
Matrix Factorization and Some Fast Discrete Transforms
Authors: Bouyukliev Iliya , Dzhumalieva-Stoeva Mariya , Piperkov Paskal
Published: July 2024

In this paper, three discrete transforms related to vector spaces over finite fields are studied. For our purposes, and according to the properties of the finite fields, the most suitable transforms are as follows: for binary fields, this is the Walsh–Hadamard transform; for odd prime fields, the Vilenkin–Chrestenson transform; and for composite fields, the trace transform. A factorization of the transform matrices using Kronecker power is given so that the considered discrete transforms are reduced to the fast discrete transforms. Examples and applications are also presented of the considered transforms in coding theory for calculating the weight distribution of a linear code.
Experimental Protocols to Test Aortic Soft Tissues: A Systematic Review
Authors: Valente Rodrigo , Mourato André , Xavier José , Sousa Pedro , Domingues Tiago , Tavares Paulo , Avril Stéphane , Tomás António , Fragata José
Published: July 2024

Experimental protocols are fundamental for quantifying the mechanical behaviour of soft tissue. These data are crucial for advancing the understanding of soft tissue mechanics, developing and calibrating constitutive models, and informing the development of more accurate and predictive computational simulations and artificial intelligence tools. This paper offers a comprehensive review of experimental tests conducted on soft aortic tissues, employing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology, based on the Scopus, Web of Science, IEEE, Google Scholar and PubMed databases. This study includes a detailed overview of the test method protocols, providing insights into practical methodologies, specimen preparation and full-field measurements. The review also briefly discusses the post-processing methods applied to extract material parameters from experimental data. In particular, the results are analysed and discussed providing representative domains of stress–strain curves for both uniaxial and biaxial tests on human aortic tissue.
Computerized Generation of Endodontic Files by Reproducing the Flute Grinding Manufacturing Process
Authors: Roda-Casanova Victor , Pérez-González Antonio
Published: July 2024

Background: File fracture during root canal treatment in endodontics is a major concern for clinicians. The strength of the file is strongly dependent on its geometry, material, and working conditions; finite element simulations are used to understand these failure mechanisms. One limitation of the models used for these simulations is the approximate geometric representation typically obtained by rotating and scaling a specific cross-section shape along the file length. Given the influence of file geometry on file strength, a more realistic representation based on the manufacturing method is needed. Methods: A computerized method was developed to generate the file geometry by simulating the flute grinding manufacturing process. This method generates the 3D geometry of the file starting from a blank and reproducing the motions of the file and grinding wheel. Results: The cross-section of the resulting geometry does not involve simple rotation and scaling but changes from the shank to the tip. The tilt angle of the grinding wheel affects the final geometry, thus altering the convexity of the cross-section. Several other parameters, such as the pitch and the radius of the grinding disc tip, impact the final geometry. Conclusions: The proposed computational method allows for the generation of endodontic file geometries that match those produced via the actual flute grinding method. This tool may help researchers and tool designers in the preparation of finite element models to assess the strength of realistic files.
Plasticity Comparison of Two Stem Cell Sources with Different Hox Gene Expression Profiles in Response to Cobalt Chloride Treatment during Chondrogenic Differentiation
Authors: Khajeh Sahar , Razban Vahid , Naeimzadeh Yasaman , Nadimi Elham , Asadi-Golshan Reza , Heidari Zahra , Talaei-Khozani Tahereh , Dehghani Farzaneh , Mostafavi-Pour Zohreh , Shirali Masoud
Published: July 2024

The limited self-repair capacity of articular cartilage is a challenge for healing injuries. While mesenchymal stem/stromal cells (MSCs) are a promising approach for tissue regeneration, the criteria for selecting a suitable cell source remain undefined. To propose a molecular criterion, dental pulp stem cells (DPSCs) with a Hox-negative expression pattern and bone marrow mesenchymal stromal cells (BMSCs), which actively express Hox genes, were differentiated towards chondrocytes in 3D pellets, employing a two-step protocol. The MSCs’ response to preconditioning by cobalt chloride (CoCl2), a hypoxia-mimicking agent, was explored in an assessment of the chondrogenic differentiation’s efficiency using morphological, histochemical, immunohistochemical, and biochemical experiments. The preconditioned DPSC pellets exhibited significantly elevated levels of collagen II and glycosaminoglycans (GAGs) and reduced levels of the hypertrophic marker collagen X. No significant effect on GAGs production was observed in the preconditioned BMSC pellets, but collagen II and collagen X levels were elevated. While preconditioning did not modify the ALP specific activity in either cell type, it was notably lower in the DPSCs differentiated pellets compared to their BMSCs counterparts. These results could be interpreted as demonstrating the higher plasticity of DPSCs compared to BMSCs, suggesting the contribution of their unique molecular characteristics, including their negative Hox expression pattern, to promote a chondrogenic differentiation potential. Consequently, DPSCs could be considered compelling candidates for future cartilage cell therapy.
Biophysical Analysis of a Minimalistic Kidney Model Expressing SGLT1 Reveals Crosstalk between Luminal and Lateral Membranes and a Plausible Mechanism of Isosmotic Transport
Authors: Larsen Erik Hviid , Sørensen Jens Nørkær
Published: July 2024

We extended our model of the S1 tubular segment to address the mechanisms by which SGLT1 interacts with lateral Na/K pumps and tight junctional complexes to generate isosmotic fluid reabsorption via tubular segment S3. The strategy applied allowed for simulation of laboratory experiments. Reproducing known experimental results constrained the range of acceptable model outputs and contributed to minimizing the free parameter space. (1) In experimental conditions, published Na and K concentrations of proximal kidney cells were found to deviate substantially from their normal physiological levels. Analysis of the mechanisms involved suggested insufficient oxygen supply as the cause and, indirectly, that a main function of the Na/H exchanger (NHE3) is to extrude protons stemming from mitochondrial energy metabolism. (2) The water path from the lumen to the peritubular space passed through aquaporins on the cell membrane and claudin-2 at paracellular tight junctions, with an additional contribution to water transport by the coupling of 1 glucose:2 Na:400 H2O in SGLT1. (3) A Na-uptake component passed through paracellular junctions via solvent drag in Na- and water-permeable claudin-2, thus bypassing the Na/K pump, in agreement with the findings of early studies. (4) Electrical crosstalk between apical rheogenic SGLT1 and lateral rheogenic Na/K pumps resulted in tight coupling of luminal glucose uptake and transepithelial water flow. (5) Isosmotic transport was achieved by Na-mediated ion recirculation at the peritubular membrane.
Plasma and Myocardial miRNomes Similarities and Differences during Cardiac Remodelling and Reverse Remodelling in a Murine Model of Heart Failure with Preserved Ejection Fraction
Authors: Thibodeau Sara-Ève , Labbé Emylie-Ann , Walsh-Wilkinson Élisabeth , Morin-Grandmont Audrey , Arsenault Marie , Couet Jacques
Published: July 2024

Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous syndrome characterised by multiple risk factors touching various organs outside the heart. Using a murine HFpEF model, we studied cardiac reverse remodelling (RR) after stopping the causing metabolic-hypertensive stress (MHS; Angiotensin II [AngII] and a high-fat diet [HFD]) after 28 days and introducing voluntary exercise (VE) for four more weeks. We measured the effects of MHS and RR on the plasma and myocardial microRNA (miR) profile (miRNome) to characterise better cardiac and non-cardiac responses to HFpEF-inducing risk factors and their reversibility. AngII alone, the HFD or the MHS caused cardiac hypertrophy (CH), left ventricular (LV) concentric remodelling and left atrial enlargement in females. Only AngII and the MHS, but not HFD, did in males. After RR, CH, LV concentric remodelling and atrial enlargement were normalised. Among the 25 most abundant circulating miRs, 10 were modulated by MHS. Plasma miRNomes from AngII, HFD or MHS mice shared 31 common significantly modulated miRs (24 upregulated and 7 downregulated), suggesting that the response of organs producing the bulk of those circulating miRs was similar even for seemingly different stress. In the LV, 19 out of 25 most expressed miRs were modulated. RR restored normality for the plasma miRNome but not for the LV miRNome, which remained mostly unchanged. Our results suggest that abnormalities persist in the myocardium of the HFpEF mice and that the normalisation of circulatory markers may be falsely reassuring after recovery.
The Autophagic Activator GHF-201 Can Alleviate Pathology in a Mouse Model and in Patient Fibroblasts of Type III Glycogenosis
Authors: Mishra Kumudesh , Sweetat Sahar , Baraghithy Saja , Sprecher Uri , Marisat Monzer , Bastu Sultan , Glickstein Hava , Tam Joseph , Rosenmann Hanna , Weil Miguel , Malfatti Edoardo , Kakhlon Or
Published: July 2024

Glycogen storage disease type III (GSDIII) is a hereditary glycogenosis caused by deficiency of the glycogen debranching enzyme (GDE), an enzyme, encoded by Agl, enabling glycogen degradation by catalyzing alpha-1,4-oligosaccharide side chain transfer and alpha-1,6-glucose cleavage. GDE deficiency causes accumulation of phosphorylase-limited dextrin, leading to liver disorder followed by fatal myopathy. Here, we tested the capacity of the new autophagosomal activator GHF-201 to alleviate disease burden by clearing pathogenic glycogen surcharge in the GSDIII mouse model Agl−/−. We used open field, grip strength, and rotarod tests for evaluating GHF-201’s effects on locomotion, a biochemistry panel to quantify hematological biomarkers, indirect calorimetry to quantify in vivo metabolism, transmission electron microscopy to quantify glycogen in muscle, and fibroblast image analysis to determine cellular features affected by GHF-201. GHF-201 was able to improve all locomotion parameters and partially reversed hypoglycemia, hyperlipidemia and liver and muscle malfunction in Agl−/− mice. Treated mice burnt carbohydrates more efficiently and showed significant improvement of aberrant ultrastructural muscle features. In GSDIII patient fibroblasts, GHF-201 restored mitochondrial membrane polarization and corrected lysosomal swelling. In conclusion, GHF-201 is a viable candidate for treating GSDIII as it recovered a wide range of its pathologies in vivo, in vitro, and ex vivo.
Novel Directed Enzyme Prodrug Therapy for Cancer Treatment Based on 2′-Deoxyribosyltransferase-Conjugated Magnetic Nanoparticles
Authors: Pérez Elena , Acosta Javier , Pisabarro Victor , Cordani Marco , dos Santos José C. S. , Sanz-Landaluze Jon , Gallo Juan , Bañobre-López Manuel , Fernández-Lucas Jesús
Published: July 2024

Directed enzyme prodrug therapy (DEPT) strategies show promise in mitigating chemotherapy side effects during cancer treatment. Among these, the use of immobilized enzymes on solid matrices as prodrug activating agents (IDEPT) presents a compelling delivery strategy, offering enhanced tumor targeting and reduced toxicity. Herein, we report a novel IDEPT strategy by employing a His-tagged Leishmania mexicana type I 2′-deoxyribosyltransferase (His-LmPDT) covalently attached to glutaraldehyde-activated magnetic iron oxide nanoparticles (MIONPs). Among the resulting derivatives, PDT-MIONP3 displayed the most favorable catalyst load/retained activity ratio, prompting its selection for further investigation. Substrate specificity studies demonstrated that PDT-MIONP3 effectively hydrolyzed a diverse array of 6-oxo and/or 6-amino purine 2′-deoxynucleosides, including 2-fluoro-2′-deoxyadenosine (dFAdo) and 6-methylpurine-2′-deoxyribose (d6MetPRib), both well-known prodrugs commonly used in DEPT. The biophysical characterization of both MIONPs and PDT-MIONPs was conducted by TEM, DLS, and single particle ICPMS techniques, showing an ideal nanosized range and a zeta potential value of −47.9 mV and −78.2 mV for MIONPs and PDT-MIONPs, respectively. The intracellular uptake of MIONPs and PDT-MIONPs was also determined by TEM and single particle ICPMS on HeLa cancer cell lines and NIH3T3 normal cell lines, showing a higher intracellular uptake in tumor cells. Finally, the selectivity of the PDT-MIONP/dFAdo IDEPT system was tested on HeLa cells (24 h, 10 µM dFAdo), resulting in a significant reduction in tumoral cell survival (11% of viability). Based on the experimental results, PDT-MIONP/dFAdo presents a novel and alternative IDEPT strategy, providing a promising avenue for cancer treatment.
Identification of Tumor Budding-Associated Genes in Breast Cancer through Transcriptomic Profiling and Network Diffusion Analysis
Authors: Janyasupab Panisa , Singhanat Kodchanan , Warnnissorn Malee , Thuwajit Peti , Suratanee Apichat , Plaimas Kitiporn , Thuwajit Chanitra
Published: July 2024

Breast cancer has the highest diagnosis rate among all cancers. Tumor budding (TB) is recognized as a recent prognostic marker. Identifying genes specific to high-TB samples is crucial for hindering tumor progression and metastasis. In this study, we utilized an RNA sequencing technique, called TempO-Seq, to profile transcriptomic data from breast cancer samples, aiming to identify biomarkers for high-TB cases. Through differential expression analysis and mutual information, we identified seven genes (NOL4, STAR, C8G, NEIL1, SLC46A3, FRMD6, and SCARF2) that are potential biomarkers in breast cancer. To gain more relevant proteins, further investigation based on a protein–protein interaction network and the network diffusion technique revealed enrichment in the Hippo signaling and Wnt signaling pathways, promoting tumor initiation, invasion, and metastasis in several cancer types. In conclusion, these novel genes, recognized as overexpressed in high-TB samples, along with their associated pathways, offer promising therapeutic targets, thus advancing treatment and diagnosis for breast cancer.
Combining Metabolomics and Machine Learning to Identify Diagnostic and Prognostic Biomarkers in Patients with Non-Small Cell Lung Cancer Pre- and Post-Radiation Therapy
Authors: Murcia-Mejía Mauricio , Canela-Capdevila Marta , García-Pablo Raquel , Jiménez-Franco Andrea , Jiménez-Aguilar Juan Manuel , Badía Joan , Benavides-Villarreal Rocío , Acosta Johana C. , Arguís Mónica , Onoiu Alina-Iuliana , Castañé Helena , Camps Jordi , Arenas Meritxell , Joven Jorge
Published: July 2024

Lung cancer is the leading cause of cancer-related deaths globally, with non-small cell lung cancer (NSCLC) accounting for over 85% of cases and poor prognosis in advanced stages. This study explored shifts in circulating metabolite levels in NSCLC patients versus healthy controls and examined the effects of conventionally fractionated radiation therapy (CFRT) and stereotactic body radiation therapy (SBRT). We enrolled 91 NSCLC patients (38 CFRT and 53 SBRT) and 40 healthy controls. Plasma metabolite levels were assessed using semi-targeted metabolomics, revealing 32 elevated and 18 reduced metabolites in patients. Key discriminatory metabolites included ethylmalonic acid, maltose, 3-phosphoglyceric acid, taurine, glutamic acid, glycocolic acid, and d-arabinose, with a combined Receiver Operating Characteristics curve indicating perfect discrimination between patients and controls. CFRT and SBRT affected different metabolites, but both changes suggested a partial normalization of energy and amino acid metabolism pathways. In conclusion, metabolomics identified distinct metabolic signatures in NSCLC patients with potential as diagnostic biomarkers. The differing metabolic responses to CFRT and SBRT reflect their unique therapeutic impacts, underscoring the utility of this technique in enhancing NSCLC diagnosis and treatment monitoring.
The Pros and Cons of Estrogens in Prostate Cancer: An Update with a Focus on Phytoestrogens
Authors: Figueira Marília I. , Carvalho Tiago M. A. , Macário-Monteiro Joana , Cardoso Henrique J. , Correia Sara , Vaz Cátia V. , Duarte Ana P. , Socorro Sílvia
Published: July 2024

The role of estrogens in prostate cancer (PCa) is shrouded in mystery, with its actions going from angelic to devilish. The findings by Huggins and Hodges establishing PCa as a hormone-sensitive cancer have provided the basis for using estrogens in therapy. However, despite the clinical efficacy in suppressing tumor growth and the panoply of experimental evidence describing its anticarcinogenic effects, estrogens were abolished from PCa treatment because of the adverse secondary effects. Notwithstanding, research work over the years has continued investigating the effects of estrogens, reporting their pros and cons in prostate carcinogenesis. In contrast with the beneficial therapeutic effects, many reports have implicated estrogens in the disruption of prostate cell fate and tissue homeostasis. On the other hand, epidemiological data demonstrating the lower incidence of PCa in Eastern countries associated with a higher consumption of phytoestrogens support the beneficial role of estrogens in counteracting cancer development. Many studies have investigated the effects of phytoestrogens and the underlying mechanisms of action, which may contribute to developing safe estrogen-based anti-PCa therapies. This review compiles the existing data on the anti- and protumorigenic actions of estrogens and summarizes the anticancer effects of several phytoestrogens, highlighting their promising features in PCa treatment.
Beyond Clinical Examination: Utilizing MRI Surveillance to Detect Recurrence of Soft Tissue Sarcomas and Differentiate from Posttherapeutic Changes
Authors: Koenig Felix R. M. , Kielburg Alfred H. , Chaudhary Snehansh Roy , Wassipaul Christian , Ganguly Akash , Varga Raoul , Watzenboeck Martin L. , Noebauer-Huhmann Iris-Melanie
Published: July 2024

Background: Early detection of soft tissue sarcoma (STS) recurrence is essential; however, the role and timeline of Magnetic resonance imaging (MRI) surveillance are still under debate. The aim of this study was to determine whether local recurrence (LR) could be identified via clinical examination alone and to assess the MRI morphology of primary STS and LR. Methods: This retrospective study included all patients with STS recurrence after surveillance for at least five years from the tumor database of the Medical University of Vienna from 2000 until December 2023. The characteristics of primary STS and LR and the time interval to recurrence and clinical detectability were assessed. The MRIs of LR and posttherapeutic changes (PTC) were compared with the initial MRIs. Results: A total of 57 patients (60% male; mean age 58.5 ± 18.0 years) with STS and histologically confirmed LR were included. The mean time interval to LR was 2.3 ± 1.8 years (range 108 to 3037 days). The clinically detectable recurrences were significantly larger than the inapparent ones (71.9 cm3 vs. 7.0 cm3; p < 0.01). The MRI morphology of all LRs (26/26) closely resembled the initial STS. For comparison, nine patients were included with clinically suspected LRs, which were histologically proven to be PTC. None of these resembled the primary STS. Conclusion: Based on clinical symptoms alone, especially small and early recurrences can be missed, which supports the importance of MRI surveillance.
Glutathione S-Transferase Gene Polymorphisms as Predictors of Methotrexate Efficacy in Juvenile Idiopathic Arthritis
Authors: Huljev Frkovic Sanda , Jelusic Marija , Crkvenac Gornik Kristina , Rogic Dunja , Frkovic Marijan
Published: July 2024

Because of the unpredictable efficacy of methotrexate (MTX) in the treatment of juvenile idiopathic arthritis (JIA), the possibility of a favourable outcome is reduced in more than 30% of patients. To investigate the possible influence of glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) gene deletion polymorphisms on MTX efficacy in patients with JIA, we determined these polymorphisms in 63 patients with JIA who did not achieve remission and 46 patients with JIA who achieved remission during MTX therapy. No significant differences were observed in the distribution of single GSTM1 or GSTT1 deletion polymorphisms or their combination between the two groups: 58.7% to 63.5%; p = 0.567, 17.4% to 22.2%; p = 0.502, and 13% to 12.7%; p = 0.966, respectively. Our results suggest that GSTM1 and GSTT1 deletion polymorphisms do not influence the efficacy of MTX in patients with JIA. Additional studies are required to determine the possible influence of GST deletion polymorphisms on MTX efficacy in patients with JIA.
Stability of the CAG Tract in the ATXN2 Gene Depends on the Localization of CAA Interruptions
Authors: Lyasota Oksana , Dorohova Anna , Hernandez-Caceres Jose Luis , Svidlov Alexandr , Tekutskaya Elena , Drobotenko Mikhail , Dzhimak Stepan
Published: July 2024

It is known that the presence of CAA codons in the CAG tract affects the nature and time of disease onset caused by the expansion of trinucleotide repeats. The mechanisms leading to the occurrence of these diseases should be sought not only at the level of the physiological role of the ATXN2 protein, but also at the DNA level. These mechanisms are associated with non-canonical configurations (hairpins) that can form in the CAG tract. The tendency of hairpins to slide along the corresponding threads is usually considered important to explain the expansion of the CAG tract. At the same time, hairpins occur in areas of open states. Previous studies on the role of CAA interruptions have suggested that, under certain conditions, they can stabilize the dynamics of the hairpin, preventing the expansion of the CAG tract. We calculated the probability of additional open state zones occurrence in the CAG tract using an angular mathematical model of DNA. The calculations made it possible to establish that CAA interruptions affect the stability of the CAG tract, and this influence, depending on the localization of the interruption, can both increase and decrease the stability of the CAG tract.
Individual Deviation-Based Functional Hypergraph for Identifying Subtypes of Autism Spectrum Disorder
Authors: Li Jialong , Zheng Weihao , Fu Xiang , Zhang Yu , Yang Songyu , Wang Ying , Zhang Zhe , Hu Bin , Xu Guojun
Published: July 2024

Heterogeneity has been one of the main barriers to understanding and treatment of autism spectrum disorder (ASD). Previous studies have identified several subtypes of ASD through unsupervised clustering analysis. However, most of them primarily depicted the pairwise similarity between individuals through second-order relationships, relying solely on patient data for their calculation. This leads to an underestimation of the complexity inherent in inter-individual relationships and the diagnostic information provided by typical development (TD). To address this, we utilized an elastic net model to construct an individual deviation-based hypergraph (ID-Hypergraph) based on functional MRI data. We then conducted a novel community detection clustering algorithm to the ID-Hypergraph, with the aim of identifying subtypes of ASD. By applying this framework to the Autism Brain Imaging Data Exchange repository data (discovery: 147/125, ASD/TD; replication: 134/132, ASD/TD), we identified four reproducible ASD subtypes with roughly similar patterns of ALFF between the discovery and replication datasets. Moreover, these subtypes significantly varied in communication domains. In addition, we achieved over 80% accuracy for the classification between these subtypes. Taken together, our study demonstrated the effectiveness of identifying subtypes of ASD through the ID-hypergraph, highlighting its potential in elucidating the heterogeneity of ASD and diagnosing ASD subtypes.
Exploring Pathways for Pain Relief in Treatment and Management of Lumbar Foraminal Stenosis: A Review of the Literature
Authors: Nurmukhametov Renat , De Jesus Encarnacion Ramirez Manuel , Dosanov Medet , Medetbek Abakirov , Kudryakov Stepan , Reyes Soto Gervith , Ponce Espinoza Claudia B. , Mukengeshay Jeff Natalaja , Mpoyi Cherubin Tshiunza , Nikolenko Vladimir , Gushcha Artem , Sharif Salman , Montemurro Nicola
Published: July 2024

Background: Lumbar foraminal stenosis (LFS) involves the narrowing of neural foramina, leading to nerve compression, significant lower back pain and radiculopathy, particularly in the aging population. Management includes physical therapy, medications and potentially invasive surgeries such as foraminotomy. Advances in diagnostic and treatment strategies are essential due to LFS’s complexity and prevalence, which underscores the importance of a multidisciplinary approach in optimizing patient outcomes. Method: This literature review on LFS employed a systematic methodology to gather and synthesize recent scientific data. A comprehensive search was conducted across PubMed, Scopus and Cochrane Library databases using specific keywords related to LFS. The search, restricted to English language articles from 1 January 2000 to 31 December 2023, focused on peer-reviewed articles, clinical trials and reviews. Due to the heterogeneity among the studies, data were qualitatively synthesized into themes related to diagnosis, treatment and pathophysiology. Results: This literature review on LFS analyzed 972 articles initially identified, from which 540 remained after removing duplicates. Following a rigorous screening process, 20 peer-reviewed articles met the inclusion criteria and were reviewed. These studies primarily focused on evaluating the diagnostic accuracy, treatment efficacy and pathophysiological insights into LFS. Conclusion: The comprehensive review underscores the necessity for precise diagnostic and management strategies for LFS, highlighting the role of a multidisciplinary approach and the utility of a unified classification system in enhancing patient outcomes in the face of this condition’s increasing prevalence.
The Impact of Continuous and Partial Reinforcement on the Acquisition and Generalization of Human-Conditioned Fear
Authors: Song Yidan , Zhao Shaochen , Rong Muxin , Liu Ying , Gao Yu , Chen Wei , Zhang Donghuan , Zheng Xifu
Published: July 2024

Fear over-generalization as a core symptom of anxiety disorders is manifested by fear responses even to safe stimuli that are very dissimilar to the original dangerous stimulus. The present study investigated the effects of two separate conditioned stimuli–unconditioned stimuli (CS–US) pairing procedures on fear acquisition and generalization using a perceptual discrimination fear-conditioning paradigm, with US expectancy ratings and skin conductance response (SCR) as indicators. One group accepted continuous followed by partial CS–US pairings (C–P group); the other group accepted partial followed by continuous CS–US pairings (P–C group). It was found that compared to the P–C group, the C–P group showed stronger perceptual discrimination of CS+ and CS− in the fear acquisition and showed weaker SCRs and stronger extinction of US expectancy in the generalization. These findings emphasize that CS–US pairings significantly influence fear acquisition and generalization and suggest that continuous-following partial CS–US pairings promote individual discrimination of threat and safety signals and inhibit the generalization of conditioned fear. The results of this study have implications for clinical interventions for patients experiencing negative events.
Exploring the Relationship between Self-Compassion and Psychological Pain: A Canonical Correlation Analysis
Authors: Garabedian Ariana , Dluzniewski Alexandra , Baker Russell T. , Casanova Madeline P.
Published: July 2024

This study investigated the association between self-compassion and psychological pain across various demographic variables. Using canonical correlation analysis, we observed an inverse relationship between the combined factors of the Self-Compassion Scale (SCS) and the Orbach and Mikulincer Mental Pain Scale (OMMP-8). Subgroup analyses revealed differences in SCS subscales among demographic groups with females, individuals with mental health diagnoses, and non-athletes displaying higher scores on negative SCS subscales and PsyPn. Injury status did not significantly affect self-compassion levels, although injured individuals scored higher on the irreversibility subscale of PsyPn. Negative SCS factors exhibited larger group differences and stronger correlations with PsyPn, indicating the potency of negative thinking in influencing psychological pain. These findings underscore the importance of self-compassion in mental health and suggest potential implications for intervention strategies.
Simulation Test of an Intelligent Vibration System for Concrete under Reinforcing Steel Mesh
Authors: Liang Hongyu , Wu Zhigang , Hu Jifeng , Gan Yuannan , Qiang Sheng
Published: July 2024

Concrete vibration construction sustains high labor intensity, a poor working environment, difficulties in quality control, and other problems. Current research on concrete vibration focuses on monitoring vibration quality, evaluating vibration processes quantitatively, and assessing mechanical vibration of unreinforced mesh concrete (plain concrete). Standardizing concrete vibration under reinforcing steel mesh remains difficult. There is still a lag in the evaluation of the quality of rework and the consumption of human and material resources. To tackle these issues, a vibrating robotic arm system based on automation control technology, machine vision, and kinematic modeling is proposed. Research and simulation tests on intelligent concrete vibration under reinforcing steel mesh aim to enhance construction efficiency and quality. A five-degree-of-freedom robotic arm with a vision module identifies each rebar grid center in the image, extracts the pixel coordinates, and converts them to the mechanical coordinates by the integration of machine vision algorithms. A vibrator point screening algorithm is introduced to determine actual vibrator point locations based on specific insertion spacing, alongside a vibro-module for vertical movement. Real-time assessment of vibration quality is achieved using the YOLOv5 target detection model. Simulation tests confirm the feasibility of automated concrete vibration control under reinforcing steel mesh by a vibrating robot arm system. This research offers a new approach for unmanned vibration technology in concrete under reinforcing steel mesh, supporting future related technological advancements with practical value.
Organizational Readiness for Building Information Modeling Implementation in Malawi: Awareness and Competence
Authors: Ndwandwe Melusi , Kuotcha Witness , Mkandawire Theresa
Published: July 2024

In developed countries, the government primarily supports the adoption of BIM. However, adoption is typically driven by the preferences of industry professionals among construction organizations in developing countries, who rely on their BIM awareness and competence. Thus, BIM competence and awareness have become an important indicator of organizational BIM readiness. To assess the level of BIM readiness, this study aims to determine the BIM competence and awareness levels among Malawian construction organizations. The study surveyed 189 construction professionals and analyzed the data using descriptive statistics and reliability tests. The findings revealed that there is a moderate level of BIM awareness among the organizations. The organizations have BIM-capable software. However, the professionals within these organizations possess a limited proficiency in BIM technical abilities, particularly in performing BIM-related services and executing BIM-related tasks. Therefore, construction organizations in Malawi should prepare for the widespread adoption of BIM by addressing the insufficient technical skills and limited awareness of BIM among their workforce. Furthermore, the study indicates that engaging in collaborative efforts could serve as a valuable foundation for the adoption of BIM within construction organizations.
Influence of Pile Foundation Construction on Existing Tunnels in a Metro Protection Area: Field Test and Numerical Simulation
Authors: Lin Gang , Ke Wenbin , Guo Shuaishuai , Lin Zhaorui , Xu Changjie , Chi Minliang , Xiao Yue
Published: July 2024

On the basis of the Fengqi Chaoming project in Hangzhou City, Zhejiang Province, China, this paper investigates the influence of pile foundation construction on the existing tunnels in a metro protection area to ensure the stability and safety of the pile foundation construction in the area of Hangzhou Metro Line 2 through in situ pile tests and numerical simulations. The test results show that the pile foundation construction has a certain influence on the existing metro tunnels, and the degree of influence gradually decreases as the distance between the pile foundation and the metro tunnel increases. The corresponding impact level for the pile foundation at 12 m from the tunnel is 1.06 mm, and that for the pile foundation at 4.9 m from the tunnel is 1.18 mm. Different types of pile foundations also lead to different degrees of influence. The maximum settlement corresponding to triaxial cement mixing piles is 1.89 mm, while the hard-method occlusal piles is 1.18 mm. The monitoring point of the metro tunnel with the smallest distance from the pile foundation experiences the largest deformation, but several sets of deformation data meet the requirements of the deformation control index, indicating that the pile foundation construction is safe and controllable.