bluuebunny/crossref_metadata_2025 huggingface dataset:
Downregulating VAC14 in Guard Cells Causes Drought Hypersensitivity by Inhibiting Stomatal Closure
Authors: Wang Zong-Qi , Liu Qi , Wu Ju-Hua , Li Juan , He Jun-Min , Zhang Yan , Li Sha
Published: January 2021

Stomata are a key land plant innovation that permit the regulation of gaseous exchanges between the plant interior and the surrounding environment. By opening or closing, stomata regulate transpiration of water though the plant; and these actions are coordinated with acquisition of CO2 for photosynthesis. Stomatal movement is controlled by various environmental and physiological factors and associates with multiple intracellular activities, among which the dynamic remodeling of vacuoles plays a crucial role. Phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2] is critical for dynamic remodeling of vacuoles. Its production requires a PI(3,5)P2-metabolizing complex consisting of FAB1/PIKfyve kinases, SAC phosphatases, and the scaffolding protein VAC14. Although genetic or pharmacological downregulation of PI(3,5)P2 causes hyposensitivity to ABA-induced stomatal closure, whether the effect of PI(3,5)P2 on stomatal movement is cell-autonomous and the physiological consequences of its reduction were unclear. We report that downregulating Arabidopsis VAC14 specifically in guard cells by artificial microRNAs (amiR-VAC14) results in enlarged guard cells and hyposensitivity to ABA- and dark-induced stomatal closure. Vacuolar fission during stomatal closure is compromised by downregulating VAC14 in guard cells. Exogenous application of PI(3,5)P2 rescued the amiR-VAC14 phenotype whereas PI(3,5)P2 inhibitor YM201636 caused wild-type plants to have inhibited stomatal closure. We further show that downregulating VAC14 specifically in guard cells impairs drought tolerance, suggestive of a key role of guard cell-produced PI(3,5)P2 in plant fitness.
A High-Density Genetic Linkage Map of SLAFs and QTL Analysis of Grain Size and Weight in Barley (Hordeum vulgare L.)
Authors: Fang Yunxia , Zhang Xiaoqin , Zhang Xian , Tong Tao , Zhang Ziling , Wu Gengwei , Hou Linlin , Zheng Junjun , Niu Chunyu , Li Jia , Wang Wenjia , Wang Hua , Xue Dawei
Published: January 2021

Grain size is an important agronomic trait determines yield in barley, and a high-density genetic map is helpful to accurately detect quantitative trait loci (QTLs) related to grain traits. Using specific-locus amplified fragment sequencing (SLAF-seq) technology, a high-density genetic map was constructed with a population of 134 recombinant inbred lines (RILs) deriving from a cross between Golden Promise (GP) and H602, which contained 12,635 SLAFs with 26,693 SNPs, and spanned 896.74 cM with an average interval of 0.07 cM on seven chromosomes. Based on the map, a total of 16 QTLs for grain length (GL), grain width and thousand-grain weight were detected on 1H, 2H, 4H, 5H, and 6H. Among them, a major QTL locusqGL1, accounting for the max phenotypic variance of 16.7% was located on 1H, which is a new unreported QTL affecting GL. In addition, the other two QTLs,qGL5andqTGW5, accounting for the max phenotypic variances of 20.7 and 21.1%, respectively, were identified in the same region, and sequencing results showed they are identical toHvDep1gene. These results indicate that it is a feasible approach to construct a high-quality genetic map for QTL mapping by using SLAF markers, and the detected major QTLsqGL1,qGL5, andqTGW5are useful for marker-assisted selection (MAS) of grain size in barley breeding.
Lexical-Semantic Development in Bilingual Toddlers at 18 and 24 Months
Authors: De Anda Stephanie , Friend Margaret
Published: January 2021

An important question in early bilingual first language acquisition concerns the development of lexical-semantic associations within and across two languages. The present study investigates the earliest emergence of lexical-semantic priming at 18 and 24 months in Spanish-English bilinguals (N= 32) and its relation to vocabulary knowledge within and across languages. Results indicate a remarkably similar pattern of development between monolingual and bilingual children, such that lexical-semantic development begins at 18 months and strengthens by 24 months. Further, measures of cross-language lexical knowledge are stronger predictors of children’s lexical-semantic processing skill than measures that capture single-language knowledge only. This suggests that children make use of both languages when processing semantic information. Together these findings inform the understanding of the relation between lexical-semantic breadth and organization in the context of dual language learners in early development.
Emotional Labor in Health Care: The Moderating Roles of Personality and the Mediating Role of Sleep on Job Performance and Satisfaction
Authors: Yeh Shu-Chuan Jennifer , Chen Shih-Hua Sarah , Yuan Kuo-Shu , Chou Willy , Wan Thomas T. H.
Published: January 2021

The objective of this study is to investigate the effects of emotional labor on job performance and satisfaction, as well as to examine the mediating effect of sleep problems and the moderating effects of personality traits. A time-lagged study was conducted on 864 health professionals. Scales for emotional labor, sleep, personality traits, and job satisfaction were used and job performance data was obtained from records maintained by human resources. Structural equation modeling was performed to investigate the relations. Sleep problems only partially mediated the relationship between surface acting and job satisfaction but completely mediated the relationship between surface acting and job performance. Several personality traits were shown to moderate the relationship between surface acting and sleep problems. The effects were stronger for people with low agreeableness and high neuroticism. The relationship between high levels of deep acting and low levels of sleep problems was more pronounced in individuals with low extraversion. Supervisors should be conscious of emotional labor in the work context and provide necessary deep acting training to facilitate emotional regulation.
Mental Health of Flying Cabin Crews: Depression, Anxiety, and Stress Before and During the COVID-19 Pandemic
Authors: Görlich Yvonne , Stadelmann Daniel
Published: January 2021

Objectives: Initially, we analyzed relations between the challenging working conditions of flight attendants with symptoms of depression, anxiety and stress. As the COVID-19 pandemic plunged airlines into an unprecedented crisis, its impact on the mental health of flying cabin crews became the focus of a second survey.

Methods: Flight attendants were surveyed online with DASS-21 in May 2019 (N = 105; sample 1) and April 2020 (N = 1119; sample 2), complemented with questions about working conditions (in 2019) and existential fears and fear of job loss (in 2020).

Results: Sample 1 revealed that symptoms of depression, anxiety and stress highly correlated with the subjective assessment of working conditions, but not with objectifiable parameters. Sample 2 showed significant positive correlations between existential fears and fear of job loss with depression, anxiety and stress. Crew members, grounded in April 2020, showed significantly higher scores in depression and stress, while still flying individuals had more clinically relevant symptoms of anxiety. Mean value comparisons between sample 1 and 2 in DASS-21 revealed a significant increase in symptoms at the time of crisis with effect sizes of d = 0.63 for depression, d = 0.26 for anxiety, and d = 0.52 for stress. The incidence of clinically relevant symptoms among the respondents increased from 8 to 23% (depression), from 6 to 14% (anxiety), and from 8 to 24% (stress).

Conclusion: The COVID-19 pandemic and associated work restrictions coincide with severe impairment of mental health of flying cabin crews, consistent with a mental health protecting function of labor.
Information Safety Assurances Increase Intentions to Use COVID-19 Contact Tracing Applications, Regardless of Autonomy-Supportive or Controlling Message Framing
Authors: Bradshaw Emma L. , Ryan Richard M. , Noetel Michael , Saeri Alexander K. , Slattery Peter , Grundy Emily , Calvo Rafael
Published: January 2021

Promoting the use of contact tracing technology will be an important step in global recovery from the COVID-19 pandemic. Across two studies, we assessed two messaging strategies as motivators of intended contact tracing uptake. In one sample of 1117 Australian adults and one sample of 888 American adults, we examined autonomy-supportive and controlling message framing and the presence or absence of information safety as predictors of intended contact tracing application uptake, using an online randomized 2 × 2 experimental design. The results suggested that the provision of data safety assurances may be key in affecting people’s intentions to use contact tracing technology, an effect we found in both samples regardless of whether messages were framed as autonomy-supportive or controlling. Those in high information safety conditions consistently reported higher intended uptake and more positive perceptions of the application than those in low information safety conditions. In Study 2, we also found that perceptions of government legitimacy related positively to intended application uptake, as did political affiliation. In sum, individuals appeared more willing to assent to authority regarding contact tracing insofar as their data safety can be assured. Yet, public messaging strategies alone may be insufficient to initiate intentions to change behavior, even in these unprecedented circumstances.
Assessing Psychometric Properties of the Italian Version of the Heartland Forgiveness Scale
Authors: Consoli Simone , Rossi Alessandro , Thompson Laura Y. , Volpi Clarissa , Mannarini Stefania , Castelnuovo Gianluca , Molinari Enrico
Published: January 2021

Despite increasing popularity and intensive worldwide use, few studies have assessed the validity and factorial structure of the Heartland Forgiveness Scale (HFS). However, scientific literature showed that the original factorial structure of the HFS was not fully replicated and—in addition—the Italian translation is still lacking. To fill this gap, this study aims to extend evidence about the original HFS factorial validity by analyzing the Italian version. The final sample was composed of 523 randomly enrolled participants [139 males (26.6%), 384 females (73.4%)] aged from 18 to 82 years (mean = 42.53, SD = 16.41) who completed the Italian version of the HFS. The confirmatory factor analysis showed good fit indices for the original hierarchical factor solution and a significant decrease in model fit was found for all of the competing models. Also, the Italian version of the HFS revealed good reliability and very good psychometrical properties. Findings suggest that the Italian version of the HFS can be considered a reliable and good psychometrically based instrument for the assessment of dispositional forgiveness of the Self, Other, and Situation.
Psychological Aspects of Students With Learning Disabilities in E-Environments: A Mini Review and Future Research Directions
Authors: Cataudella Stefania , Carta Stefano , Mascia Maria Lidia , Masala Carmelo , Petretto Donatella Rita , Penna Maria Pietronilla
Published: January 2021

What are the main learning difficulties or advantages encountered by students with learning disabilities (LDs) within e-environments? As a result of the Covid-19 emergency, e-learning is being increasingly used to support students’ learning processes. A number of countries closed their schools altogether, so face-to-face lessons were and have been replaced by distance lessons. A search of current literature via Scopus, Eric and Google Scholar electronic databases was conducted according to Prisma Guidelines. Other sources of literature were also considered, starting from the references in the full text of the articles consulted. We used the following search keywords: “LDs” combined with the “AND/OR” Boolean operator and “e-learning platforms,” “well-being,” “psychological factors,” “emotional distress,” and “self-regulation.” One body of literature highlights the lack of inclusive accessibility standards and a lack of attention to specific tools for addressing LDs, which causes students to develop high levels of stress/anxiety and emotional distress, in addition to low levels of well-being, self-esteem and self-efficacy. Another area of literature looks at how students can develop high levels of self-regulation and emotional awareness, as well as high levels of inclusion. Results are discussed in terms of the promotion of e-learning that focuses on the psychological well-being of students and teachers use of technological tools.
The Effect of LPS and Ketoprofen on Cytokines, Brain Monoamines, and Social Behavior in Group-Housed Pigs
Authors: Veit Christina , Janczak Andrew M. , Ranheim Birgit , Vas Judit , Valros Anna , Sandercock Dale A. , Piepponen Petteri , Dulgheriu Daniela , Nordgreen Janicke
Published: January 2021

Poor health is a risk factor for damaging behaviors, but the mechanisms behind this link are unknown. Injection of pigs with lipopolysaccharide (LPS) can be used to model aspects of poor health. Recent studies have shown that LPS-injected pigs perform more tail- and ear-directed behavior compared to saline-injected pigs and suggest that pro-inflammatory cytokines may play a role in these behaviors. The aims of this study were to test the effect of LPS on the social behavior of pigs and the neurotransmitters and modulators in their brains and to test the effect of a nonsteroidal anti-inflammatory drug on the effects of LPS. Fifty-two female pigs (11–12 weeks) were allocated to four treatments comprising two injections: saline–saline (SS), saline–LPS (SL), ketoprofen–saline (KS), and ketoprofen–LPS (KL). Activity was scan-sampled every 5 min for 6 h after the last injection in the pen. Social behavior was observed continuously in 10 × 15-min bouts between 8 a.m. and 5 p.m. 1 day before (baseline) and 1 and 2 days after the injection. Saliva was analyzed for cortisol and plasma for tryptophan and kynurenine. The frontal cortex, hippocampus, hypothalamus, and brain stem were sampled 72 h after the injection and analyzed for cytokines and monoamines. LPS activated the HPA axis and decreased the activity within 6 h after the injection. Ketoprofen lowered the effect of LPS on cortisol release and attenuated the behavioral signs of sickness in challenged pigs. SL pigs manipulated the ears of their pen mates significantly longer than SS pigs 2 days after the injection. LPS had no observed effect on IFN-γ, TNF-α, and IL-18. At 72 h after the injection, plasma tryptophan was depleted in SL pigs, and tryptophan and kynurenine concentrations in the frontal cortex and brain stem of SL pigs were significantly lower compared to those in SS pigs. Dopamine concentrations in the hypothalamus of SL pigs were significantly lower compared to those in SS pigs. Serotonin concentrations in the hypothalamus and noradrenaline concentrations in the hippocampus of SL pigs were significantly lower compared to those in KL pigs. In conclusion, LPS influenced the different neurotransmitters and modulators in the brain that are hypothesized to play an important role in the regulation of mood and behavior.
Molecular Characterization of Chelonid Alphaherpesvirus 5 in a Black Turtle (Chelonia mydas) Fibropapilloma from Baja California Sur, Mexico
Authors: Reséndiz Eduardo , Fernández-Sanz Helena , Domínguez-Contreras José Francisco , Ramos-Díaz Amelly Hyldaí , Mancini Agnese , Zavala-Norzagaray Alan A. , Aguirre A. Alonso
Published: January 2021

During routine monitoring in Ojo de Liebre Lagoon, Mexico, a juvenile black turtle (Chelonia mydas) was captured, physically examined, measured, weighed, sampled, and tagged. The turtle showed no clinical signs suggestive of disease. Eleven months later, this turtle was recaptured in the same area, during which one lesion suggestive of fibropapilloma on the neck was identified and sampled for histopathology and molecular analysis. Histopathology revealed hyperkeratosis, epidermal hyperplasia, acanthosis, papillary differentiation and ballooning degeneration of epidermal cells, increased fibroblasts in the dermis, and angiogenesis, among other things. Hematological values were similar to those reported for clinically healthy black turtles and did not show notable changes between the first capture and the recapture; likewise, clinicopathological evaluation did not show structural or functional damage in the turtle’s systems. The chelonid alphaherpesvirus 5 (ChHV5) UL30 gene was amplified and sequenced for phylogeny; Bayesian reconstruction showed a high alignment with the genus Scutavirus of the Eastern Pacific group. This is one of the first reports of ChHV5 in a cutaneous fibropapilloma of a black turtle in the Baja California peninsula.
Oxidative Stress in the Pathogenesis of Crohn’s Disease and the Interconnection with Immunological Response, Microbiota, External Environmental Factors, and Epigenetics
Authors: Alemany-Cosme Ester , Sáez-González Esteban , Moret Inés , Mateos Beatriz , Iborra Marisa , Nos Pilar , Sandoval Juan , Beltrán Belén
Published: January 2021

Inflammatory bowel disease (IBD) is a complex multifactorial disorder in which external and environmental factors have a large influence on its onset and development, especially in genetically susceptible individuals. Crohn’s disease (CD), one of the two types of IBD, is characterized by transmural inflammation, which is most frequently located in the region of the terminal ileum. Oxidative stress, caused by an overabundance of reactive oxygen species, is present locally and systemically in patients with CD and appears to be associated with the well-described imbalanced immune response and dysbiosis in the disease. Oxidative stress could also underlie some of the environmental risk factors proposed for CD. Although the exact etiopathology of CD remains unknown, the key role of oxidative stress in the pathogenesis of CD is extensively recognized. Epigenetics can provide a link between environmental factors and genetics, and numerous epigenetic changes associated with certain environmental risk factors, microbiota, and inflammation are reported in CD. Further attention needs to be focused on whether these epigenetic changes also have a primary role in the pathogenesis of CD, along with oxidative stress.
Data Analytics for Profiling Low-Voltage Customers with Smart Meter Readings
Authors: Pilo Fabrizio , Pisano Giuditta , Ruggeri Simona , Troncia Matteo
Published: January 2021

The energy transition for decarbonization requires consumers’ and producers’ active participation to give the power system the necessary flexibility to manage intermittency and non-programmability of renewable energy sources. The accurate knowledge of the energy demand of every single customer is crucial for accurately assessing their potential as flexibility providers. This topic gained terrific input from the widespread deployment of smart meters and the continuous development of data analytics and artificial intelligence. The paper proposes a new technique based on advanced data analytics to analyze the data registered by smart meters to associate to each customer a typical load profile (LP). Different LPs are assigned to low voltage (LV) customers belonging to nominal homogeneous category for overcoming the inaccuracy due to non-existent coincident peaks, arising by the common use of a unique LP per category. The proposed methodology, starting from two large databases, constituted by tens of thousands of customers of different categories, clusters their consumption profiles to define new representative LPs, without a priori preferring a specific clustering technique but using that one that provides better results. The paper also proposes a method for associating the proper LP to new or not monitored customers, considering only few features easily available for the distribution systems operator (DSO).
Trends in Performance Limits of the HOT Infrared Photodetectors
Authors: Rogalski Antoni , Martyniuk Piotr , Kopytko Małgorzata , Hu Weida
Published: January 2021

The cryogenic cooling of infrared (IR) photon detectors optimized for the mid- (MWIR, 3–5 µm) and long wavelength (LWIR, 8–14 µm) range is required to reach high performance. This is a major obstacle for more extensive use of IR technology. Focal plane arrays (FPAs) based on thermal detectors are presently used in staring thermal imagers operating at room temperature. However, their performance is modest; thermal detectors exhibit slow response, and the multispectral detection is difficult to reach. Initial efforts to develop high operating temperature (HOT) photodetectors were focused on HgCdTe photoconductors and photoelectromagnetic detectors. The technological efforts have been lately directed on advanced heterojunction photovoltaic HgCdTe detectors. This paper presents the several approaches to increase the photon-detectors room-temperature performance. Various kinds of materials are considered: HgCdTe, type-II AIIIBV superlattices, two-dimensional materials and colloidal quantum dots.
Transitioning Broadcast to Cloud
Authors: Reznik Yuriy , Cenzano Jordi , Zhang Bo
Published: January 2021

We analyze the differences between on-premise broadcast and cloud-based online video delivery workflows and identify technologies needed for bridging the gaps between them. Specifically, we note differences in ingest protocols, media formats, signal-processing chains, codec constraints, metadata, transport formats, delays, and means for implementing operations such as ad-splicing, redundancy and synchronization. To bridge the gaps, we suggest specific improvements in cloud ingest, signal processing, and transcoding stacks. Cloud playout is also identified as critically needed technology for convergence. Finally, based on all such considerations, we offer sketches of several possible hybrid architectures, with different degrees of offloading of processing in cloud, that are likely to emerge in the future.
Electronic Structure Correspondence of Singlet-Triplet Scale Separation in Strained Sr2RuO4
Authors: Acharya Swagata , Pashov Dimitar , Chachkarova Elena , Schilfgaarde Mark van , Weber Cédric
Published: January 2021

At a temperature of roughly 1 K, Sr2RuO4 undergoes a transition from a normal Fermi liquid to a superconducting phase. Even while the former is relatively simple and well understood, the superconducting state has not even been understood after 25 years of study. More recently, it has been found that critical temperatures can be enhanced by the application of uniaxial strain, up to a critical strain, after which it falls off. In this work, we take an “instability” approach and seek divergences in susceptibilities. This provides an unbiased way to distinguish tendencies to competing ground states. We show that in the unstrained compound, the singlet and triplet instabilities of the normal Fermi liquid phase are closely spaced. Under uniaxial strain, electrons residing on all orbitals contributing to the Fermiology become more coherent, while the electrons of the Ru-dxy character become heavier, and the electrons of the Ru-dxz,yz characters become lighter. In the process, Im χ(q,ω) increases rapidly around q = (0.3,0.3,0)2π/a and q = (0.5,0.25,0)2π/a, while it gets suppressed at all other commensurate vectors, in particular at q = 0, which is essential for spin-triplet superconductivity. We observe that the magnetic anisotropy under strain drops smoothly, which is concomitant with the increment in singlet instability. Thus, the triplet superconducting instability remains the lagging instability of the system, and the singlet instability enhances under strain, leading to a large energy-scale separation between these competing instabilities. However, since this happens even without spin-orbit coupling, we believe it is primarily the enhancement in the spin fluctuation glue around quasi-anti-ferromagnetic vectors that drives the Cooper pairing instead of the magnetic anisotropy. At large strain, an instability to a spin density wave overtakes the superconducting one. The analysis relies on a high-fidelity, ab initio description of the one-particle properties and two-particle susceptibilities, based on the quasiparticle self-consistent GW approximation augmented by dynamical mean field theory. This approach is described and its high fidelity confirmed by comparing to observed one- and two-particle properties.
The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers
Authors: Mikheeva Elza R. , Katraeva Inna V. , Kovalev Andrey A. , Kovalev Dmitriy A. , Nozhevnikova Alla N. , Panchenko Vladimir , Fiore Ugo , Litti Yuri V.
Published: January 2021

This article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogen-scavengers in the inoculum (mesophilic and thermophilic anaerobic sludge). Acid pretreatment was effective for thermophilic anaerobic sludge to suppress methanogenic activity, and heat treatment was effective for mesophilic anaerobic sludge. Maximum specific yields of hydrogen, namely 178 mL/g chemical oxygen demand (COD) and 149 mL/g COD for AFB and AF, respectively, were obtained at the hydraulic retention time (HRT) of 4.5 days and organic load rate (OLR) of 6.61 kg COD/(m3 day). At the same time, the maximum hydrogen production rates of 1.28 and 1.9 NL/(L day) for AF and AFB, respectively, were obtained at the HRT of 2.02 days and OLR of 14.88 kg COD/(m3 day). At the phylum level, the dominant taxa were Firmicutes (65% in AF and 60% in AFB), and at the genus level, Lactobacillus (40% in AF and 43% in AFB) and Bifidobacterium (24% in AF and 30% in AFB).
Evaluation of Coupling PV and Air Conditioning vs. Solar Cooling Systems—Case Study from Jordan
Authors: Albatayneh Aiman , Jaradat Mustafa , Al-Omary Murad , Zaquot Maha
Published: January 2021

When they were first conceived, solar cooling systems were designed to be cost-effective and environmentally safe alternatives for the majority of the developing nations that are characterised by their hot climates in contrast with the traditional air conditioning systems powered by electricity that is produced from fossil fuel resources. Nevertheless, developments in photovoltaic (PV) and air-conditioning technologies have impacted on the prospects of solar cooling systems. This study examined two different options: a coupled PV and air conditioner system and a solar cooling system (absorption chillers where thermal energy is provided by solar collectors) for a specific developing country located in the Eastern Mediterranean region whose climate is hot and dry (Jordan). The cooling system comprised a pair of cooled multistage compression, both of which were 700 kW, while the PV system’s size was 2.1 MWp, the utility grid connection was a 0.4 kV 50 Hz net meter (2 m) and it was anticipated that 3300 MWh/year would be generated. The solar cooling system operated at a maximum coefficient of performance (COP) of 0.79 and had an actual recorded COP of 0.32 on the site; when the electricity tariff of $0.1/kWh was considered, the respective levelised cost of energy (LCOE) values were $0.9/kWh and $2.35/kWh respectively. The findings indicate that the initial costs for the solar thermal cooling system and the PV system were approximately $3.150M and $3M, respectively. The current value of future cash payments when discounts of 6% per year were applied to the payments for the combination of PV and air conditioning was about $9,745,000, whereas the solar thermal cooling system will not reach the breakeven point at negative $1,730,000. It is clear the absorption chiller did not display economic feasibility, whereas the value for the coupled PV and air-conditioning systems was under $0.05/kWh. In addition to the extensive maintenance needs, the reduced COP and the practicality and feasibility of the solar thermal cooling systems mean these kinds of technologies are under significant pressure to remain competitive when faced with the development of new air conditioning and PV technologies.
Structure and Density of Sedimentary Basins in the Southern Part of the East-European Platform and Surrounding Area
Authors: Kaban Mikhail K. , Gvishiani Alexei , Sidorov Roman , Oshchenko Alexei , Krasnoperov Roman I.
Published: January 2021

Modern satellite gravity missions and ground gravimetry provide operational data models that can be used in various studies in geology, tectonics, and climatology, etc. In the present study, sedimentary basins in the southern part of the East European Platform and adjoining areas including the Caucasus are studied by employing the approach based on decompensative gravity anomalies. The new model of sediments, implying their thickness and density, demonstrates several important features of the sedimentary cover, which were not or differently imaged by previous studies. We found a significant redistribution of the low-dense sediments in the Black Sea. Another principal feature is the increased thickness of relatively low-dense sediments in the Eastern Greater Caucasus. The deepest part of the South Caspian basin is shifted to the north, close to the Apsheron Trough. In its present position, it is almost joined with the Terek–Caspian depression, which depth is also increased. The thickness of sediments is significantly decreased in the eastern Pre-Caspian basin. Therefore, the new sedimentary cover model gives a more detailed description of its thickness and density, reveals new features and helps in better understanding of the evolution of the basins, providing a background for further detailed studies of the region.
Study of Temporal Variations of Equivalent Black Carbon in a Coastal City in Northwest Spain Using an Atmospheric Aerosol Data Management Software
Authors: Piñeiro-Iglesias María , Andrade-Garda Javier , Suárez-Garaboa Sonia , Muniategui-Lorenzo Soledad , López-Mahía Purificación , Prada-Rodríguez Darío
Published: January 2021

Light-absorbing carbonaceous aerosols (including black carbon (BC)) pose serious health issues and play significant roles in atmospheric radiative properties. Two-year measurements (2015–2016) of aerosol light absorption, combined with measurements of sub-micrometric particles, were continuously conducted in A Coruña (northwest (NW) Spain) to determine their light absorption properties: absorption coefficients (σabs) and the absorption Ångström exponent (AAE). The mean and standard deviation of equivalent black carbon (eBC) during the period of study were 0.85 ± 0.83 µg m−3, which are lower than other values measured in urban areas of Spain and Europe. High eBC concentrations found in winter are associated with an increase in emissions from anthropogenic sources in combination with lower mixing layer heights and frequent stagnant conditions. The pronounced diurnal variability suggests a strong influence from local sources. AAE had an average value of 1.26 ± 0.22 which implies that both fossil fuel combustion and biomass burning influenced optical aerosol properties. This also highlights biomass combustion in suburban areas, where the use of wood for domestic heating is encouraged, as an important source of eBC. All data treatment was gathered using SCALA© as atmospheric aerosol data management support software program.
Impact of Plant Origin on Eurasian Propolis on Phenolic Profile and Classical Antioxidant Activity
Authors: Okińczyc Piotr , Widelski Jarosław , Szperlik Jakub , Żuk Magdalena , Mroczek Tomasz , Skalicka-Woźniak Krystyna , Sakipova Zuriyadda , Widelska Gabriela , Kuś Piotr Marek
Published: January 2021

Propolis is a bee product with known medical properties, including antioxidant activity. The scope of the study is profiling 19 different Eurasian propolis samples (mostly from Russia and Kazakhstan, Kyrgyzstan, Poland, Ukraine, and Slovakia). Profiles of propolises were investigated by ultra-high-performance liquid chromatography–diode array detector–mass spectrometry (UPLC-DAD-MS). Classical antioxidant properties, which are based on electron donation mechanism, were assessed by DPPH, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays. Total phenolic and flavonoid contents were also evaluated by colorimetric tests. Most of the samples exhibited significant content of polyphenols (from 30.28 to 145.24 mg GAE/g of propolis) and flavonoids (from 10.45 to 82.71 mg GAE/g of propolis). Most of the propolis samples exhibited potent antiradical (DPPH test—from 8.83 to 64.47 mg GAE/g of propolis) and reducing activity (FRAP test—from 0.08 to 1.17 mmol Fe2+/g of propolis). Based on the occurrence of marker compounds, propolis samples were classified as poplar, aspen–birch, aspen–poplar, and aspen–birch–poplar type. Main markers present in propolis of poplar (e.g., chrysin, pinocembrin, galangin, and 3-O-acetyl-pinobanksin), birch (ermanin and acacetin) and aspen (2-acetyl-1,3-di-p-coumaroylglycerol) origin were used. DPPH, FRAP, and ORAC tests results were correlated with flavonoids, total polyphenols, or the polyphenols other than flavonoids content. In term of activity, poplar propolis type was variable, while aspen–birch–poplar type usually exhibited high DPPH and FRAP activity.
The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction
Authors: Wanschel Amarylis C. B. A. , Guizoni Daniele M. , Lorza-Gil Estela , Salerno Alessandro G. , Paiva Adriene A. , Dorighello Gabriel G. , Davel Ana Paula , Balkan Wayne , Hare Joshua M. , Oliveira Helena C. F.
Published: January 2021

Endothelial dysfunction precedes atherosclerosis and is an independent predictor of cardiovascular events. Cholesterol levels and oxidative stress are key contributors to endothelial damage, whereas high levels of plasma high-density lipoproteins (HDL) could prevent it. Cholesteryl ester transfer protein (CETP) is one of the most potent endogenous negative regulators of HDL-cholesterol. However, whether and to what degree CETP expression impacts endothelial function, and the molecular mechanisms underlying the vascular effects of CETP on endothelial cells, have not been addressed. Acetylcholine-induced endothelium-dependent relaxation of aortic rings was impaired in human CETP-expressing transgenic mice, compared to their non-transgenic littermates. However, endothelial nitric oxide synthase (eNOS) activation was enhanced. The generation of superoxide and hydrogen peroxide was increased in aortas from CETP transgenic mice, while silencing CETP in cultured human aortic endothelial cells effectively decreased oxidative stress promoted by all major sources of ROS: mitochondria and NOX2. The endoplasmic reticulum stress markers, known as GADD153, PERK, and ARF6, and unfolded protein response effectors, were also diminished. Silencing CETP reduced endothelial tumor necrosis factor (TNF) α levels, intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) expression, diminishing monocyte adhesion. These results support the notion that CETP expression negatively impacts endothelial cell function, revealing a new mechanism that might contribute to atherosclerosis.
Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell
Authors: Chansaenpak Kantapat , Kamkaew Anyanee , Lisnund Sireerat , Prachai Pannaporn , Ratwirunkit Patipat , Jingpho Thitichaya , Blay Vincent , Pinyou Piyanut
Published: January 2021

Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1–7.0 mM, which is perfectly suited for common biofluids and industrial food samples.
“Imagine You Have ALS”: Death Education to Prepare for Advance Treatment Directives
Authors: Testoni Ines , Palazzo Lorenza , Calamarà Nicoletta , Rossi Gabriella , Wieser Michael Alexander
Published: January 2021

The study presents the results of qualitative research carried out within a death education project dedicated to advance treatment directives (ATDs) in which it was proposed to participants to empathize with people who had received a diagnosis of Amyotrophic Lateral Sclerosis (ALS). The study involved 104 people who discussed and reflected on issues related to the knowledge of having to die, palliative care and ATDs, investigating what choices they would have made if they had received such a diagnosis. Finally, they were asked to write a paper describing their impressions and hypothetical choices. Qualitative analysis has elucidated among fundamental themes. Four thematic areas emerged from the data analysis: (1) ATDs and the family; (2) the importance of reducing pain and suffering; (3) emotions and considerations regarding death, illness and spirituality; and (4) opinions on the DeEd course. It has emerged that some people are unfamiliar with palliative care or the right to self-determination and that addressing these issues helps manage the thought of the future with less terror. The experience of death education has therefore proven to be very positive in dealing with complex and often censored issues, allowing thinking about death in a less distressing way.
How Long Can a Wood Flooring System Last?
Authors: Coelho Pedro , Silva Ana , de Brito Jorge
Published: January 2021

Wood is a natural, sustainable, and renewable material, which has been used as flooring for centuries, but not enough is known about its durability and performance over time when subjected to different degradation agents. This study proposes a methodology for the service life prediction of wood flooring systems, considering the impact of different factors that influence the floors’ durability. For that purpose, a fieldwork survey is performed to evaluate the degradation phenomena of 96 indoor wood floorings in-use conditions, located in Portugal. The data collected are converted into degradation patterns that graphically illustrate the loss of performance of wood floorings over time. An estimated service life of 44 years is obtained. This study thus allows quantifying the impact of various characteristics on the indoor wood floorings’ service life. The results reveal the high importance of the type of protection, the type of wood, and the type of floor (with a range of estimated service life values of around 18, 17 and 16 years, respectively). This study is a first step to understanding the degradation mechanisms of the wood flooring systems, in order to extend their service life, while allowing optimising of maintenance actions, thus promoting the durability and sustainability of these floorings.
Immunologic Pathophysiology and Airway Remodeling Mechanism in Severe Asthma: Focused on IgE-Mediated Pathways
Authors: Cheng Shih-Lung
Published: January 2021

Despite the expansion of the understanding in asthma pathophysiology and the continual advances in disease management, a small subgroup of patients remains partially controlled or refractory to standard treatments. Upon the identification of immunoglobulin E (IgE) and other inflammatory mediators, investigations and developments of targeted agents have thrived. Omalizumab is a humanized monoclonal antibody that specifically targets the circulating IgE, which in turn impedes and reduces subsequent releases of the proinflammatory mediators. In the past decade, omalizumab has been proven to be efficacious and well-tolerated in the treatment of moderate-to-severe asthma in both trials and real-life studies, most notably in reducing exacerbation rates and corticosteroid use. While growing evidence has demonstrated that omalizumab may be potentially beneficial in treating other allergic diseases, its indication remains confined to treating severe allergic asthma and chronic idiopathic urticaria. Future efforts may be bestowed on determining the optimal length of omalizumab treatment, seeking biomarkers that could better predict treatment response and as well as extending its indications.
An Entropic Approach to Estimating the Instability Criterion of People in Floodwaters
Authors: Zhu Zhongfan , Zhang Yongpeng , Gou Lufeng , Pang Bo
Published: January 2021

People are always susceptible to a loss of stability in urban floodwaters that leads to serious casualties. Thus, the safety criterion for the instability of people in floodwaters must be determined. In this study, the hydrodynamic criterion of the instability of people in floodwaters in terms of the incipient velocity and water depth is derived using the probability method based on Shannon entropy theory. The derived model can characterize variations in the incipient velocity of people in floodwaters with respect to the inundating water depth. Furthermore, a comparison with seven experimental datasets available in the literature shows the validity of the proposed entropy-based model considering data scattering. A sensitivity analysis of the derived model to some of the incorporated parameters was performed, and the qualitative results are in accordance with our understanding of the physical mechanism of the instability of people in floodwaters. Taking the physical parameters (height and mass) of Chinese adults and children as a representative example, this study also showed the vulnerability degree of Chinese adults and children subject to floodwaters. These findings could provide a reference for administrators and stakeholders for flood hazard mitigation and flood strategy management. This study shows that an entropy-based method could be a valuable addition to existing deterministic models for characterizing the instability criterion of people in an urban flooding event.
Matrix Product State Simulations of Non-Equilibrium Steady States and Transient Heat Flows in the Two-Bath Spin-Boson Model at Finite Temperatures
Authors: Dunnett Angus J. , Chin Alex W.
Published: January 2021

Simulating the non-perturbative and non-Markovian dynamics of open quantum systems is a very challenging many body problem, due to the need to evolve both the system and its environments on an equal footing. Tensor network and matrix product states (MPS) have emerged as powerful tools for open system models, but the numerical resources required to treat finite-temperature environments grow extremely rapidly and limit their applications. In this study we use time-dependent variational evolution of MPS to explore the striking theory of Tamascelli et al. (Phys. Rev. Lett. 2019, 123, 090402.) that shows how finite-temperature open dynamics can be obtained from zero temperature, i.e., pure wave function, simulations. Using this approach, we produce a benchmark dataset for the dynamics of the Ohmic spin-boson model across a wide range of coupling strengths and temperatures, and also present a detailed analysis of the numerical costs of simulating non-equilibrium steady states, such as those emerging from the non-perturbative coupling of a qubit to baths at different temperatures. Despite ever-growing resource requirements, we find that converged non-perturbative results can be obtained, and we discuss a number of recent ideas and numerical techniques that should allow wide application of MPS to complex open quantum systems.
Additive Manufacturing and Topology Optimization of Magnetic Materials for Electrical Machines—A Review
Authors: Pham Thang , Kwon Patrick , Foster Shanelle
Published: January 2021

Additive manufacturing has many advantages over traditional manufacturing methods and has been increasingly used in medical, aerospace, and automotive applications. The flexibility of additive manufacturing technologies to fabricate complex geometries from copper, polymer, and ferrous materials presents unique opportunities for new design concepts and improved machine power density without significantly increasing production and prototyping cost. Topology optimization investigates the optimal distribution of single or multiple materials within a defined design space, and can lead to unique geometries not realizable with conventional optimization techniques. As an enabling technology, additive manufacturing provides an opportunity for machine designers to overcome the current manufacturing limitation that inhibit adoption of topology optimization. Successful integration of additive manufacturing and topology optimization for fabricating magnetic components for electrical machines can enable new tools for electrical machine designers. This article presents a comprehensive review of the latest achievements in the application of additive manufacturing, topology optimization, and their integration for electrical machines and their magnetic components.
New Pathways for Community Energy and Storage
Authors: Koirala Binod , van Oost Ellen , van der Waal Esther , van der Windt Henny
Published: January 2021

Worldwide, the energy landscape is changing [...]
Upstream Regulator Analysis of Wooden Breast Myopathy Proteomics in Commercial Broilers and Comparison to Feed Efficiency Proteomics in Pedigree Male Broilers
Authors: Bottje Walter G. , Lassiter Kentu R. , Kuttappan Vivek A. , Hudson Nicholas J. , Owens Casey M. , Abasht Behnam , Dridi Sami , Kong Byungwhi C.
Published: January 2021

In an effort to understand the apparent trade-off between the continual push for growth performance and the recent emergence of muscle pathologies, shotgun proteomics was conducted on breast muscle obtained at ~8 weeks from commercial broilers with wooden breast (WB) myopathy and compared with that in pedigree male (PedM) broilers exhibiting high feed efficiency (FE). Comparison of the two proteomic datasets was facilitated using the overlay function of Ingenuity Pathway Analysis (IPA) (Qiagen, CA, USA). We focused on upstream regulator analysis and disease-function analysis that provides predictions of activation or inhibition of molecules based on (a) expression of downstream target molecules, (b) the IPA scientific citation database. Angiopoeitin 2 (ANGPT2) exhibited the highest predicted activation Z-score of all molecules in the WB dataset, suggesting that the proteomic landscape of WB myopathy would promote vascularization. Overlaying the FE proteomics data on the WB ANGPT2 upstream regulator network presented no commonality of protein expression and no prediction of ANGPT2 activation. Peroxisome proliferator coactivator 1 alpha (PGC1α) was predicted to be inhibited, suggesting that mitochondrial biogenesis was suppressed in WB. PGC1α was predicted to be activated in high FE pedigree male broilers. Whereas RICTOR (rapamycin independent companion of mammalian target of rapamycin) was predicted to be inhibited in both WB and FE datasets, the predictions were based on different downstream molecules. Other transcription factors predicted to be activated in WB muscle included epidermal growth factor (EGFR), X box binding protein (XBP1), transforming growth factor beta 1 (TGFB1) and nuclear factor (erythroid-derived 2)-like 2 (NFE2L2). Inhibitions of aryl hydrocarbon receptor (AHR), AHR nuclear translocator (ARNT) and estrogen related receptor gamma (ESRRG) were also predicted in the WB muscle. These findings indicate that there are considerable differences in upstream regulators based on downstream protein expression observed in WB myopathy and in high FE PedM broilers that may provide additional insight into the etiology of WB myopathy.