bluuebunny/crossref_metadata_2025 huggingface dataset:
Next-Generation Human Liver Models for Antimalarial Drug Assays
Authors: Kulkeaw Kasem
Published: May 2021

Advances in malaria prevention and treatment have significantly reduced the related morbidity and mortality worldwide, however, malaria continues to be a major threat to global public health. Because Plasmodium parasites reside in the liver prior to the appearance of clinical manifestations caused by intraerythrocytic development, the Plasmodium liver stage represents a vulnerable therapeutic target to prevent progression. Currently, a small number of drugs targeting liver-stage parasites are available, but all cause lethal side effects in glucose-6-phosphate dehydrogenase-deficient individuals, emphasizing the necessity for new drug development. Nevertheless, a longstanding hurdle to developing new drugs is the availability of appropriate in vitro cultures, the crucial conventional platform for evaluating the efficacy and toxicity of drugs in the preclinical phase. Most current cell culture systems rely primarily on growing immortalized or cancerous cells in the form of a two-dimensional monolayer, which is not very physiologically relevant to the complex cellular architecture of the human body. Although primary human cells are more relevant to human physiology, they are mainly hindered by batch-to-batch variation, limited supplies, and ethical issues. Advances in stem cell technologies and multidimensional culture have allowed the modelling of human infectious diseases. Here, current in vitro hepatic models and toolboxes for assaying the antimalarial drug activity are summarized. Given the physiological potential of pluripotent and adult stem cells to model liver-stage malaria, the opportunities and challenges in drug development against liver-stage malaria is highlighted, paving the way to assess the efficacy of hepatic plasmodicidal activity.
Pharmacological and Clinical Significance of Heme Oxygenase-1
Authors: Stec David E. , Abraham Nader G.
Published: May 2021

This Special Issue collates and updates the current knowledge of the pharmacology and clinical applications concerning the enzyme heme oxygenase (HO) [...]
(–)-Loliolide Isolated from Sargassum horneri Suppressed Oxidative Stress and Inflammation by Activating Nrf2/HO-1 Signaling in IFN-γ/TNF-α-Stimulated HaCaT Keratinocytes
Authors: Han Eui-Jeong , Fernando Ilekuttige Priyan Shanura , Kim Hyun-Soo , Lee Dae-Sung , Kim Areum , Je Jun-Geon , Seo Min-Jeong , Jee Young-Heun , Jeon You-Jin , Kim Seo-Young , Ahn Ginnae
Published: May 2021

The present study evaluated the effects of (–)-loliolide isolated from Sargassum horneri (S. horneri) against oxidative stress and inflammation, and its biological mechanism in interferon (IFN)-γ/tumor necrosis factor (TNF)-α-stimulated HaCaT keratinocytes. The results showed that (–)-loliolide improved the cell viability by reducing the production of intracellular reactive oxygen species (ROS) in IFN-γ/TNF-α-stimulated HaCaT keratinocytes. In addition, (–)-loliolide effectively decreased the expression of inflammatory cytokines (interleukin (IL)-4 IL-6, IL-13, IFN-γ and TNF-α) and chemokines (CCL11 (Eotaxin), macrophage-derived chemokine (MDC), regulated on activation, normal T cell expressed and secreted (RANTES), and thymus and activation-regulated chemokine (TARC)), by downregulating the expression of epidermal-derived initial cytokines (IL-25, IL-33 and thymic stromal lymphopoietin (TSLP)). Furthermore, (–)-loliolide suppressed the activation of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) signaling, whereas it activated nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Interestingly, the cytoprotective effects of (–)-loliolide against IFN-γ/TNF-α stimulation were significantly blocked upon inhibition of HO-1. Taken together, these results suggest that (–)-loliolide effectively suppressed the oxidative stress and inflammation by activating the Nrf2/HO-1 signaling in IFN-γ/TNF-α-stimulated HaCaT keratinocytes.
Natural Antioxidant Application on Fat Accumulation: Preclinical Evidence
Authors: Roy Proshanta , Tomassoni Daniele , Traini Enea , Martinelli Ilenia , Micioni Di Bonaventura Maria Vittoria , Cifani Carlo , Amenta Francesco , Tayebati Seyed Khosrow
Published: May 2021

Obesity represents one of the most important challenges in the contemporary world that must be overcome. Different pathological consequences of these physical conditions have been studied for more than 30 years. The most nagging effects were found early in the cardiovascular system. However, later, its negative impact was also investigated in several other organs. Damage at cellular structures due to overexpression of reactive oxygen species together with mechanisms that cause under-production of antioxidants leads to the development of obesity-related complications. In this view, the negative results of oxidant molecules due to obesity were studied in various districts of the body. In the last ten years, scientific literature has reported reasonable evidence regarding natural and synthetic compounds’ supplementation, which showed benefits in reducing oxidative stress and inflammatory processes in animal models of obesity. This article attempts to clarify the role of oxidative stress due to obesity and the opposing role of antioxidants to counter it, reported in preclinical studies. This analysis aims to clear-up different mechanisms that lead to the build-up of pro-oxidants during obesity and how various molecules of different origins hinder this phenomenon, behaving as antioxidants.
Cardamonin Attenuates Inflammation and Oxidative Stress in Interleukin-1β-Stimulated Osteoarthritis Chondrocyte through the Nrf2 Pathway
Authors: Peng Yi-Jen , Lu Jeng-Wei , Lee Chian-Her , Lee Herng-Sheng , Chu You-Hsiang , Ho Yi-Jung , Liu Feng-Cheng , Huang Chun-Jung , Wu Chia-Chun , Wang Chih-Chien
Published: May 2021

Osteoarthritis (OA) is a chronic degenerative joint disease characterized by the deterioration of articular cartilage. The progression of OA leads to an increase in inflammatory mediators in the joints, thereby promoting the destruction of the cartilage matrix. Recent studies have reported on the anti-inflammatory and antioxidant properties of cardamonin, which also appears to interact with cellular targets, such as nuclear erythroid 2-related factor 2 (Nrf2), extracellular signal-regulated kinase (ERK), and mammalian target of rapamycin (mTOR) during the progression of tumors. To date, few studies have investigated the effects of cardamonin on chondrocyte inflammation. In the current study, we determined that treating interleukin-1 beta (IL-1β-stimulated chondrocyte cells) with cardamonin significantly reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) and significantly inhibited the expression of pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2). Cardamonin was also shown to: (1) inhibit the activation and production of matrix metalloproteinases (MMPs), (2) suppress the nuclear factor-κB (NF-κB) signaling pathway, (3) suppress the expression of toll-like receptor proteins, (4) activate the Nrf2 signaling pathway, and (5) increase the levels of antioxidant proteins heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1). The increase in antioxidant proteins led to corresponding antioxidant effects (which were abolished by Nrf2 siRNA). Our findings identify cardamonin as a candidate Nrf2 activator for the treatment and prevention of OA related to inflammation and oxidative stress.
Influence of Various Types of Office Desk Chair for Dynamizing the Operation Assessed by Raster Stereography
Authors: Dulina Ľuboslav , Gola Arkadiusz , Gašo Martin , Horváthová Blanka , Bigošová Eleonóra , Barbušová Miroslava , Plinta Dariusz , Kyncl Jiří
Published: May 2021

The current development trend of the operational activities indicates an increase in occupations which last for a few generations and whose primary position is the activity in the sitting position. This trend is directly connected with the technological progress and development of the society within Industry 4.0. However, the workplaces intended for sitting occupations that are designed according to the current standards are unsuitable from several perspectives. The long-term sitting activities at most actual workplaces cause an accumulation of the static load. For this reason, the article deals with dynamizing the activities in the sitting position. The presented research is based on utilizing the knowledge of the sitting posture dynamics and on the information acquired through diagnostics based on the Raster Stereography as an innovative method based on modelling a natural human spin. The article brings the first research results, which points out the fact that probably not all types of dynamic sitting that are nowadays preferred are suitable for long-term working.
The Effect of Transcutaneous Application of Gaseous CO2 on Diabetic Symmetrical Peripheral Neuropathy—A Double-Blind Randomized Clinical Trial
Authors: Ban Frangež Helena , Rodi Zoran , Miklavčič Jana , Frangež Igor
Published: May 2021

Aim: Diabetic symmetrical peripheral neuropathy is a common complication of diabetes mellitus. Patients treated with transcutaneous CO2 application for chronic wounds reported an improvement in peripheral sensations. This study aimed to evaluate the effect of transcutaneous application of gaseous CO2 on diabetic symmetrical peripheral neuropathy. Methods: A prospective randomized, double-blind study was performed at the University Medical Center Ljubljana between September 2019 and September 2020. Sixty consecutive patients with diabetes with a unilateral chronic wound were randomized into either a study group that received transcutaneous CO2 therapy or a control group that received placebo treatment with air. Results: Vibration, monofilament sensation, and temperature of the big toe improved significantly in the study group (p < 0.001, for vibration sensation, monofilament test and temperature of the big toe), but not in the control group (p = ns for all evaluated outcomes). Conclusion: According to our results, a transcutaneous application of gaseous CO2 shows promising results in treating diabetic symmetrical peripheral neuropathy. Considering the major consequences of sensory loss leading to foot ulceration and possibly amputation, we believe this treatment approach deserves future attention and investigation as a treatment modality of diabetic symmetrical peripheral neuropathy.
Applications of Nanosized-Lipid-Based Drug Delivery Systems in Wound Care
Authors: Matei Andreea-Mariana , Caruntu Constantin , Tampa Mircea , Georgescu Simona Roxana , Matei Clara , Constantin Maria Magdalena , Constantin Traian Vasile , Calina Daniela , Ciubotaru Diana Alina , Badarau Ioana Anca , Scheau Cristian , Caruntu Ana
Published: May 2021

Impaired wound healing is an encumbering public health issue that increases the demand for developing new therapies in order to minimize health costs and enhance treatment efficacy. Available conventional therapies are still unable to maximize their potential in penetrating the skin at the target site and accelerating the healing process. Nanotechnology exhibits an excellent opportunity to enrich currently available medical treatments, enhance standard care and manage wounds. It is a promising approach, able to address issues such as the permeability and bioavailability of drugs with reduced stability or low water solubility. This paper focuses on nanosized-lipid-based drug delivery systems, describing their numerous applications in managing skin wounds. We also highlight the relationship between the physicochemical characteristics of nanosized, lipid-based drug delivery systems and their impact on the wound-healing process. Different types of nanosized-lipid-based drug delivery systems, such as vesicular systems and lipid nanoparticles, demonstrated better applicability and enhanced skin penetration in wound healing therapy compared with conventional treatments. Moreover, an improved chemically and physically stable drug delivery system, with increased drug loading capacity and enhanced bioavailability, has been shown in drugs encapsulated in lipid nanoparticles. Their applications in wound care show potential for overcoming impediments, such as the inadequate bioavailability of active agents with low solubility. Future research in nanosized-lipid-based drug delivery systems will allow the achievement of increased bioavailability and better control of drug release, providing the clinician with more effective therapies for wound care.
Numerical Study of the Hydrodynamic Characteristics Comparison between a Ducted Propeller and a Rim-Driven Thruster
Authors: Liu Bao , Vanierschot Maarten
Published: May 2021

The Rim-Driven Thruster (RDT) is an extraordinary innovation in marine propulsion applications. The structure of an RDT resembles a Ducted Propeller (DP), as both contain several propeller blades and a duct shroud. However, unlike the DP, there is no tip clearance in the RDT as the propeller is directly connected to the rim. Instead, a gap clearance exists in the RDT between the rim and the duct. The distinctive difference in structure between the DP and the RDT causes significant discrepancy in the performance and flow features. The present work compares the hydrodynamic performance of a DP and an RDT by means of Computational Fluid Dynamics (CFD). Reynolds-Averaged Navier–Stokes (RANS) equations are solved in combination with an SST k-ω turbulence model. Validation and verification of the CFD model is conducted to ensure the numerical accuracy. Steady-state simulations are carried out for a wide range of advance coefficients with the Moving Reference Frame (MRF) approach. The results show that the gap flow in the RDT plays an important role in affecting the performance. Compared to the DP, the RDT produces less thrust on the propeller and duct, and, because of the existence of the rim, the overall efficiency of the RDT is significantly lower than the one of the ducted propeller.
fNIRS Signal Classification Based on Deep Learning in Rock-Paper-Scissors Imagery Task
Authors: Ma Tengfei , Chen Wentian , Li Xin , Xia Yuting , Zhu Xinhua , He Sailing
Published: May 2021

To explore whether the brain contains pattern differences in the rock–paper–scissors (RPS) imagery task, this paper attempts to classify this task using fNIRS and deep learning. In this study, we designed an RPS task with a total duration of 25 min and 40 s, and recruited 22 volunteers for the experiment. We used the fNIRS acquisition device (FOIRE-3000) to record the cerebral neural activities of these participants in the RPS task. The time series classification (TSC) algorithm was introduced into the time-domain fNIRS signal classification. Experiments show that CNN-based TSC methods can achieve 97% accuracy in RPS classification. CNN-based TSC method is suitable for the classification of fNIRS signals in RPS motor imagery tasks, and may find new application directions for the development of brain–computer interfaces (BCI).
Superfluid Transition and Specific Heat of the 2D x-y Model: Monte Carlo Simulation
Authors: Nguyen Phong H. , Boninsegni Massimo
Published: May 2021

We present results of large-scale Monte Carlo simulations of the 2D classical x-y model on the square lattice. We obtain high accuracy results for the superfluid fraction and for the specific heat as a function of temperature, for systems of size L×L with L up to 212. Our estimate for the superfluid transition temperature is consistent with those furnished in all previous studies. The specific heat displays a well-defined peak, whose shape and position are independent of the size of the lattice for L>28, within the statistical uncertainties of our calculations. The implications of these results on the interpretation of experiments on adsorbed thin films of 4He are discussed.
Driving Behavior Modeling Based on Consistent Variable Selection in a PWARX Model
Authors: Nwadiuto Jude Chibuike , Okuda Hiroyuki , Suzuki Tatsuya
Published: May 2021

This paper proposes the hybrid system model identified by a PWARX (piecewise affine autoregressive exogenous) model for modeling human driving behavior. In the proposed model, the mode segmentation is carried out automatically and the optimal number of modes is decided by a novel methodology based on consistent variable selection. In addition, model flexibility is added within the ARX (autoregressive exogenous) partitions in the form of statistical variable selection. The proposed method is able to capture both the decision-making and motion-control facets of the driving behavior. The resulting model is an optimal basal model which is not affected by the choice of data, where the explanatory variables are allowed to vary within each ARX region, thus, allowing a higher-level understanding of the motion-control aspect of the driving behavior, as well as explaining the driver’s decision-making. The proposed model is applied to model the car-following driving task based on real-road driving data, as well as to ROS-CARLA-based car-following simulation and compared to Gipp’s driver model. Obtained results that show better performance both on prediction performance and mimicking actual real-road driving demonstrates and validates the usefulness of the model.
A Hybrid Nanofluid of Alumina and Tungsten Oxide for Performance Enhancement of a Parabolic Trough Collector under the Weather Conditions of Budapest
Authors: Al-Oran Otabeh , Lezsovits Ferenc
Published: May 2021

Recently, there has been significant interest in the thermal performance of parabolic trough collectors. They are capable of operating and generating highly variable temperature ranges, which can be used in various applications. This paper, therefore, addressed the thermal performance model of using a parabolic trough collector under the radiation intensity level found in Budapest city, as well as the effect of inserting a hybrid nanofluid as the thermal fluid. First, a new modified hybrid nanofluid of alumina and tungsten oxide-based Therminol VP1 is used to enhance the thermal properties of the thermal fluid to be more efficient to use. This enhancement is performed under various volume concentrations and has a volume fraction of 50:50. Second, in order to demonstrate the effectiveness of the thermal element, mathematical energy balance equations were solved and simulated using MATLAB Symbolic Tools. The simulation is presented for two cases: one under a constant radiation intensity and the other under the radiation intensity level of Budapest. For both cases, the results of the dimensionless Nusselt number, heat transfer coefficient, pressure drop, exergy efficiency, and energy efficiency are described. The major findings show that a volume concentration of 4% (Al2O3 and WO3) based Therminol VP1 was the most efficient volume concentrations in both cases. For the first case, the maximum enhancement of the Nusselt number and the heat transfer coefficient are 138% and 169%, respectively. These results enhanced the thermal and exergy efficiencies by 0.39% and 0.385% at a temperature 600 K, flow rate of 150 L/min, and radiation intensity of 1000 W/m2. For the second case, the maximum exergy and energy values are recorded at midday under Budapest’s summer climatic conditions and reach 32.728% and 71.255%, respectively, under the optimum temperature of 500 K and flow rate of 150 L/min. Accordingly, the mean improvement in thermal and exergy efficiencies approximately equal to 0.25% at a high concentration, regardless of the season (summer or winter).
Evaluation of Heating Technique of Deformed Reinforcement Using High-Frequency Induction Heating System
Authors: Lim Myung-kwan , Lee Changhee
Published: May 2021

To improve recycling quality, it is necessary to develop a demolition technology that can be combined with existing crushing methods that employ large shredding-efficient equipment. The efficient collection of bones in a segmentation dismantling method must be considered according to the procedure. Furthermore, there is a need for the development of partial dismantling technologies that enable efficient remodeling, maintenance, and reinforcement. In this study, we experimentally investigated the temperature-rise characteristics of reinforced concrete through partial rapid heating during high-frequency induced heating. Accordingly, the chemical and physical vulnerability characteristics of the reinforced concrete were verified by studying the thermal conduction on the surface of the rebars and the cracks caused by the thermal expansion pressure of the rebars. Furthermore, we aimed to verify the applicability of the proposed technology by specifying the vulnerability range of the reinforced concrete based on the heating range, as well as the appropriate energy consumption. We investigated the temperature rise and temperature distribution characteristics of the rebar surfaces based on diameter, length, bar placement conditions, heating distance, heating coil location, and output, using reinforced steel of grade SD345. Maximum powers of 5, 6, and 10 kW, and inductive heating were used to achieve satisfactory results.
Need for Standardization: Influence of Artificial Canal Size on Cyclic Fatigue Tests of Endodontic Instruments
Authors: Bürklein Sebastian , Maßmann Paul , Donnermeyer David , Tegtmeyer Karsten , Schäfer Edgar
Published: May 2021

The aim was to evaluate the influence of artificial canal size on the results of cyclic fatigue tests for endodontic instruments. Dynamic cyclic fatigue at body temperature using continuous tapered nickel–titanium F6-SkyTaper instruments (Komet, Lemgo, Germany), size 25/.06 with an amplitude of 3 mm, was tested in four different simulated root canals: (A) size of the instrument +0.02 mm (within the tolerances of the instruments); (B) +0.05 mm; (C) +0.10 mm; (D) parallel tube with 1.25 mm in diameter. The artificial canals (angle of curvature 60°, radius 5.0 mm, center of curvature 5.0 mm) were produced by a LASER-melting technique. Time and cycles to fracture, and lengths of the fractured instruments were recorded and statistically analyzed (Student–Newman–Keuls; Kruskal–Wallis test). Time to fracture significantly increased with increasing size of the artificial canals in the following order: A < B, C < D (p < 0.05). Length of separated instruments continuously decreased with increasing canal sizes. The parallel tube produced the significantly shortest fragments (p < 0.05). Within the limitations of this study, dynamic cyclic fatigue of endodontic instruments depends on the congruency of the instruments’ dimensions with that of the artificial canals. In future cyclic fatigue testing, due to the closer match of canal and instrument parameters, it is necessary to adjust the artificial canal sizes to the size of the instruments within the manufacturing tolerances of the instruments.
Intra- and Inter-Rater Reliability of a Well-Used and a Less-Used IsoMed 2000 Dynamometer for Knee Flexion and Extension Peak Torque Measurements in a Concentric Test in Athletes
Authors: Kocahan Tuğba , Akınoğlu Bihter , Yilmaz Ayfer Ezgi , Rosemann Thomas , Knechtle Beat
Published: May 2021

This study aimed to evaluate the intra- and inter-rater reliability of a well-used and a less-used IsoMed-2000 dynamometer for knee flexion and extension peak torque (PT) measurements in a concentric test in athletes. Two IsoMed-2000 isokinetic dynamometers were used, an old one that was well-used and a new one that was less-used. Thirty-seven weight-lifting athletes (12 female, 25 male) were included in this study. Both legs of each athlete were tested twice on each isokinetic dynamometer at 60°/s and 180°/s. The Intraclass Correlation Coefficients (ICC), Standard Error Measurement (SEM), Smallest Detectable Change (SDC), SDC% and 95% limits of agreement were calculated to evaluate the intra- and inter-rater reliability of a well-used and a less-used IsoMed 2000 dynamometer for knee flexion and extension peak torque in athletes. Intra-rater reliability was moderate to excellent (ICCs: 0.516–0.928) for knee muscle strength parameters, and the highest SDC and SEM values were 0.76 N/kg and 0.28 N/kg, respectively. Inter-rater reliability was moderate to excellent (ICCs: 0.519–915) for knee muscle strength parameters, and the highest SDC and SEM values were 0.81 N/kg and 0.29 N/kg, respectively. At least 89.2% of the obtained values of parameters fell within the 95% limits of agreement. Our findings demonstrate the intra and inter-rater reliability of a Well-Used and a Less-Used IsoMed 2000 Dynamometer for Knee Flexion and Extension Peak Torque in Athletes.
Characteristics of the Soil Microbial Communities in Different Slope Positions along an Inverted Stone Slope in a Degraded Karst Tiankeng
Authors: Jiang Cong , Feng Jie , Zhu Su-Feng , Shui Wei
Published: May 2021

The underground forests developed on inverted stone slopes in degraded karst tiankengs are important areas for biodiversity conservation, but the microbial community profiles have not been sufficiently characterized. Thus, we investigated the soil microbial communities at four sites (at the bottom of the slope (BS), in the middle of the slope (MS), in the upper part of the slope (US) and outside the tiankeng (OT)) in the Shenxiantang tiankeng. The dominant phyla in the inverted stone slope were Proteobacteria, Actinobacteria, and Acidobacteria, and the relative abundance were different in different slope positions. The Shannon–Wiener diversity index of the microbial community was significantly greater for the US site than for the MS or BS sites. The metabolism functional pathways (including C/N cycle) were more abundant at the BS site. Total nitrogen and pH were the dominant factors in determining the distribution of the microbial community along an inverted stone slope. These results suggest that topographic heterogeneity can influence the variations in the soil microbial structure, diversity, and function in degraded karst tiankengs and emphasized the ecological value of inverted stone slopes within karst tiankengs.
Nigella and Milk Thistle Seed Oils: Potential Cytoprotective Effects against 7β-Hydroxycholesterol-Induced Toxicity on SH-SY5Y Cells
Authors: Hammouda Souha , Ghzaiel Imen , Picón-Pagès Pol , Meddeb Wiem , Khamlaoui Wided , Hammami Sonia , Muñoz Francisco J. , Hammami Mohamed , Zarrouk Amira
Published: May 2021

Oxysterols are assumed to be the driving force behind numerous neurodegenerative diseases. In this work, we aimed to study the ability of 7β-hydroxycholesterol (7β-OHC) to trigger oxidative stress and cell death in human neuroblastoma cells (SH-SY5Y) then the capacity of Nigella sativa and Milk thistle seed oils (NSO and MTSO, respectively) to oppose 7β-OHC-induced side effects. The impact of 7β-OHC, associated or not with NSO or MTSO, was studied on different criteria: cell viability; redox status, and apoptosis. Oxidative stress was assessed through the intracellular reactive oxygen species (ROS) production, levels of enzymatic and non-enzymatic antioxidants, lipid, and protein oxidation products. Our results indicate that 7β-OHC (40 µg/mL) exhibit pr-oxidative and pro-apoptotic activities shown by a decrease of the antioxidant enzymatic activities and an increase of ROS production, lipid, and protein oxidation end products as well as nitrotyrosine formation and caspase 3 activation. However, under the pre-treatment with NSO, and especially with MTSO (100 µg/mL), a marked attenuation of oxidative damages was observed. Our study suggests harmful effects of 7β-OHC consisting of pro-oxidative, anti-proliferative, and pro-apoptotic activities that may contribute to neurodegeneration. NSO and especially MTSO showed potential cytoprotection against the cytotoxicity of 7β-OHC.
Parents’ Perceptions of Educational Apps Use for Kindergarten Children: Development and Validation of a New Instrument (PEAU-p) and Exploration of Parents’ Profiles
Authors: Vaiopoulou Julie , Papadakis Stamatios , Sifaki Eirini , Stamovlasis Dimitrios , Kalogiannakis Michail
Published: May 2021

Contemporary mobile technologies offer tablets and smartphones that elicit young children’s active participation in various educational apps, dramatically transforming playing, learning, and communication. Even the most knowledgeable users face difficulties in deciding about the value and appropriateness of the so-called educational apps because of many factors that should be considered. Their importance for children’s attitudes is affected by the perceived positive and negative aspects, which vary across a multiplicity of criteria. Filling the gap in the relevant literature, a new instrument, named PEAU-p (Perceptions about Educational Apps Use–parents), was developed and validated in the present study designed to measure parents’ perception of educational apps for kindergarten pupils. Data (N = 435) were collected via online procedures, and the psychometric properties of PEAU-p were studied via exploratory and confirmatory methods. Principal Components Analysis extracted six factors, namely Usability, Enjoyment, Involvement, Learning, Worries, and Values, which explained 72.42% of the total variance. Subsequently, by implementing Latent Class Analysis based on the above factors, four Clusters (i.e., parents’ Profiles) were extracted corresponding to their perceptions and attitudes towards the educational apps used for kindergarten pupils. Those were named as ‘mild attitude’, ‘negative attitude’, ‘positive attitude’, and ‘indifferent attitude’. This categorization, besides the statistical support, is fully interpretable, and the profiles were associated with certain covariates, such as age, the number of children, knowledge on new technologies, or distal outcomes, e.g., the frequency of using apps, the general position towards apps or their intention to recommend apps use. The findings are discussed within the current research field, investigating the influential role parents play in young children’s media selection and use.
Bioreactor and Bioprocess Design Issues in Enzymatic Hydrolysis of Lignocellulosic Biomass
Authors: Olivieri Giuseppe , Wijffels René H. , Marzocchella Antonio , Russo Maria Elena
Published: May 2021

Saccharification of lignocellulosic biomass is a fundamental step in the biorefinery of second generation feedstock. The physicochemical and enzymatic processes for the depolymerization of biomass into simple sugars has been achieved through numerous studies in several disciplines. The present review discusses the development of technologies for enzymatic saccharification in industrial processes. The kinetics of cellulolytic enzymes involved in polysaccharide hydrolysis has been discussed as the starting point for the design of the most promising bioreactor configurations. The main process configurations—proposed so far—for biomass saccharification have been analyzed. Attention was paid to bioreactor configurations, operating modes and possible integrations of this operation within the biorefinery. The focus is on minimizing the effects of product inhibition on enzymes, maximizing yields and concentration of sugars in the hydrolysate, and reducing the impact of enzyme cost on the whole process. The last part of the review is focused on an emerging process based on the catalytic action of laccase applied to lignin depolymerization as an alternative to the consolidated physicochemical pretreatments. The laccases-based oxidative process has been discussed in terms of characteristics that can affect the development of a bioreactor unit where laccases or a laccase-mediator system can be used for biomass delignification.
Epitaxial Growth of Single Crystal YAG for Optical Devices
Authors: Qadri Syed N. , Kim Woohong , Bayya Shyam , Shaw L. Brandon , Qadri Syed B. , Kolis Joseph , Stadelman Bradley , Sanghera Jasbinder
Published: May 2021

We report the latest progress on fabrication of rare earth doped single crystal yttrium aluminum garnet (YAG) core/undoped YAG cladded fibers. Rare-earth doped single crystal core fibers were grown with laser heated pedestal growth methods. In a second step, epitaxial methods were used to grow a single crystalline undoped YAG cladding onto the core fiber. Hydrothermal and liquid phase epitaxy methods utilize the core doped fiber as the seed. X-ray diffraction of cladding reveals an equilibrium (110) morphology. Energy-dispersive X-ray spectroscopy analysis shows there is minimal diffusion of rare-earth dopants into the cladding structure. The use of scandium doping is shown to substitute at the Al3+ site, thereby allowing an additional tunability of refractive index of core structure material besides conventional Y3+ site dopants. The use of these epitaxial growth methods enables material compatibility, tuning of refractive index, and conformal growth of cladding structures onto core fibers for optical devices.
EIPPM—The Executable Integrative Product-Production Model
Authors: Schopper Dominik , Kübler Karl , Rudolph Stephan , Riedel Oliver
Published: May 2021

In this paper, a combination of graph-based design and simulation-based engineering (SBE) into a new concept called Executable Integrative Product-Production Model (EIPPM) is elaborated. Today, the first collaborative process in engineering for all mechatronic disciplines is the virtual commissioning phase. The authors see a hitherto untapped potential for the earlier, integrated and iterative use of SBE for the development of production systems (PS). Seamless generation of and exchange between Model-, Software- and Hardware-in-the-Loop simulations is necessary. Feedback from simulation results will go into the design decisions after each iteration. The presented approach combines knowledge of the domain “PSs” together with the knowledge of the corresponding “product” using a so called Graph-based Design Language (GBDL). Its central data model, which represents the entire life cycle of product and PS, results of an automatic translation step in a compiler. Since the execution of the GBDL can be repeated as often as desired with modified boundary conditions (e.g., through feedback), a design of experiment is made possible, whereby unconventional solutions are also considered. The novel concept aims at the following advantages: Consistent linking of all mechatronic disciplines through a data model (graph) from the project start, automatic design cycles exploring multiple variants for optimized product-PS combinations, automatic generation of simulation models starting with the planning phase and feedback from simulation-based optimization back into the data model.
Morphology and Position of the Right Atrioventricular Valve in Relation to Right Atrial Structures
Authors: Hołda Jakub , Słodowska Katarzyna , Malinowska Karolina , Strona Marcin , Mazur Małgorzata , Jasińska Katarzyna A. , Matuszyk Aleksandra , Koziej Mateusz , Walocha Jerzy A. , Hołda Mateusz K.
Published: May 2021

The right atrioventricular valve (RAV) is an important anatomical structure that prevents blood backflow from the right ventricle to the right atrium. The complex anatomy of the RAV has lowered the success rate of surgical and transcatheter procedures performed within the area. The aim of this study was to describe the morphology of the RAV and determine its spatial position in relation to selected structures of the right atrium. We examined 200 randomly selected human adult hearts. All leaflets and commissures were identified and measured. The position of the RAV was defined. Notably, 3-leaflet configurations were present in 67.0% of cases, whereas 4-leaflet configurations were present in 33.0%. Septal and mural leaflets were both significantly shorter and higher in 4-leaflet than in 3-leaflet RAVs. Significant domination of the muro-septal commissure in 3-leflet valves was noted. The supero-septal commissure was the most stable point within RAV circumference. In 3-leaflet valves, the muro-septal commissure was placed within the cavo-tricuspid isthmus area in 52.2% of cases, followed by the right atrial appendage vestibule region (20.9%). In 4-leaflet RAVs, the infero-septal commissure was located predominantly in the cavo-tricuspid isthmus area and infero-mural commissure was always located within the right atrial appendage vestibule region. The RAV is a highly variable structure. The supero-septal part of the RAV is the least variable component, whereas the infero-mural is the most variable. The number of detected RAV leaflets significantly influences the relative position of individual valve components in relation to right atrial structures.
Effect of Multishell Diffusion MRI Acquisition Strategy and Parcellation Scale on Rich-Club Organization of Human Brain Structural Networks
Authors: Khalilian Maedeh , Kazemi Kamran , Fouladivanda Mahshid , Makki Malek , Helfroush Mohammad Sadegh , Aarabi Ardalan
Published: May 2021

The majority of network studies of human brain structural connectivity are based on single-shell diffusion-weighted imaging (DWI) data. Recent advances in imaging hardware and software capabilities have made it possible to acquire multishell (b-values) high-quality data required for better characterization of white-matter crossing-fiber microstructures. The purpose of this study was to investigate the extent to which brain structural organization and network topology are affected by the choice of diffusion magnetic resonance imaging (MRI) acquisition strategy and parcellation scale. We performed graph-theoretical network analysis using DWI data from 35 Human Connectome Project subjects. Our study compared four single-shell (b = 1000, 3000, 5000, 10,000 s/mm2) and multishell sampling schemes and six parcellation scales (68, 200, 400, 600, 800, 1000 nodes) using five graph metrics, including small-worldness, clustering coefficient, characteristic path length, modularity and global efficiency. Rich-club analysis was also performed to explore the rich-club organization of brain structural networks. Our results showed that the parcellation scale and imaging protocol have significant effects on the network attributes, with the parcellation scale having a substantially larger effect. Regardless of the parcellation scale, the brain structural networks exhibited a rich-club organization with similar cortical distributions across the parcellation scales involving at least 400 nodes. Compared to single b-value diffusion acquisitions, the deterministic tractography using multishell diffusion imaging data consisting of shells with b-values higher than 5000 s/mm2 resulted in significantly improved fiber-tracking results at the locations where fiber bundles cross each other. Brain structural networks constructed using the multishell acquisition scheme including high b-values also exhibited significantly shorter characteristic path lengths, higher global efficiency and lower modularity. Our results showed that both parcellation scale and sampling protocol can significantly impact the rich-club organization of brain structural networks. Therefore, caution should be taken concerning the reproducibility of connectivity results with regard to the parcellation scale and sampling scheme.
Secured Big Data Analytics for Decision-Oriented Medical System Using Internet of Things
Authors: Rehman Amjad , Haseeb Khalid , Saba Tanzila , Lloret Jaime , Tariq Usman
Published: May 2021

The Internet of Medical Things (IoMT) has shown incredible development with the growth of medical systems using wireless information technologies. Medical devices are biosensors that can integrate with physical things to make smarter healthcare applications that are collaborated on the Internet. In recent decades, many applications have been designed to monitor the physical health of patients and support expert teams for appropriate treatment. The medical devices are attached to patients’ bodies and connected with a cloud computing system for obtaining and analyzing healthcare data. However, such medical devices operate on battery powered sensors with limiting constraints in terms of memory, transmission, and processing resources. Many healthcare solutions are helping the community with the efficient monitoring of patients’ conditions using cloud computing, however, mostly incur latency in data collection and storage. Therefore, this paper presents a model for the Secured Big Data analytics using Edge–Cloud architecture (SBD-EC), which aims to provide distributed and timely computation of a decision-oriented medical system. Moreover, the mobile edges cooperate with the cloud level to present a secure algorithm, achieving reliable availability of medical data with privacy and security against malicious actions. The performance of the proposed model is evaluated in simulations and the results obtained demonstrate significant improvement over other solutions.
Physiological, Morphological and Antioxidant Responses of Pediococcus pentosaceus R1 and Lactobacillus fermentum R6 Isolated from Harbin Dry Sausages to Oxidative Stress
Authors: Zhang Huan , Xu Jianhang , Chen Qian , Wang Hui , Kong Baohua
Published: May 2021

As functional starter cultures and potential probiotics, the ability of lactic acid bacteria to resist oxidative stress is essential to maintain viability and functional properties. This study investigates the effects of H2O2 at different concentrations (0, 1, 2, and 3 mM) on the physiological, morphological, and antioxidant properties of Pediococcus pentosaceus R1 and Lactobacillus fermentum R6 isolated from Harbin dry sausages. The increase in H2O2 concentration induced a significant increase in reactive oxygen species and a decrease in intracellular ATP levels (p < 0.05). Based on scanning electron microscopy, transmission electron microscopy, and electric conductivity analysis, H2O2 stress caused cell deformation, the destruction of cell membrane integrity, partial loss of the cytoplasm, and an increase in the cell conductivity of both strains. H2O2 stress with 1 mM or 2 mM concentrations could effectively improve the scavenging rates of free radicals, the activities of superoxide dismutase and glutathione peroxide, and the total antioxidant capacity of both strains (p < 0.05). In conclusion, an appropriate oxidative stress contributed to the activation of the antioxidant defense system of both strains, conferred strains a better effect in inhibiting the oxidation of fermented foods, and improved the health of the host.
Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q0 against Salmonella Typhimurium
Authors: Yang Zhuokai , Ma Xiaoyu , Li Yan , Xu Huidong , Han Xinyi , Wang Ruixia , Zhao Pengyu , Li Ziyi , Shi Chao
Published: May 2021

Coenzyme Q0 (CoQ0) has anti-inflammatory and anti-tumor effects; however, the antimicrobial and antibiofilm activities of CoQ0 against Salmonella enterica serovar Typhimurium are unknown. Thus, we investigated the bacteriostatic and antibiofilm activities, along with the underlying mechanism, of CoQ0 against S. Typhimurium. The minimum inhibitory concentration (MIC) of CoQ0 against S. enterica serovars Typhimurium was 0.1–0.2 mg/mL (549–1098 µM), and CoQ0 at MIC and 2MIC decreased viable S. Typhimurium counts below detectable limits within 6 and 4 h, respectively. CoQ0 at 20MIC (4 mg/mL) reduced S. Typhimurium on raw chicken by 1.5 log CFU/cm3 within 6 h. CoQ0 effectively disrupted cell membrane integrity and induced morphological changes in the cell, resulting in hyperpolarization, decreased intracellular ATP concentrations, and cellular constituents leakage. Biofilm-associated S. Typhimurium cells were killed by CoQ0 treatment. These findings suggest that CoQ0 could be applied as a natural antibacterial substance for use against S. Typhimurium by the food industry.
How Will the Future of Work Shape OSH Research and Practice? A Workshop Summary
Authors: Felknor Sarah , Streit Jessica , McDaniel Michelle , Schulte Paul , Chosewood L. , Delclos George ,   on behalf of the Workshop Presenters and Participants
Published: May 2021

Growth of the information economy and globalization of labor markets will be marked by exponential growth in emerging technologies that will cause considerable disruption of the social and economic sectors that drive the global job market. These disruptions will alter the way we work, where we work, and will be further affected by the changing demographic characteristics and level of training of the available workforce. These changes will likely result in scenarios where existing workplace hazards are exacerbated and new hazards with unknown health effects are created. The pace of these changes heralds an urgent need for a proactive approach to understand the potential effects new and emerging workplace hazards will have on worker health, safety, and well-being. As employers increasingly rely on non-standard work arrangements, research is needed to better understand the work organization and employment models that best support decent work and improved worker health, safety, and well-being. This need has been made more acute by the SARS-CoV-2 global pandemic that has resulted in dramatic changes in employment patterns, millions of lost jobs, an erosion of many economic sectors, and widespread disparities which further challenge occupational safety and health (OSH) systems to ensure a healthy and productive workplace. To help identify new research approaches to address OSH challenges in the future, a virtual workshop was organized in June 2020 with leading experts in the fields of OSH, well-being, research methods, mental health, economics, and life-course analysis. A paradigm shift will be needed for OSH research in the future of work that embraces key stakeholders and thinks differently about research that will improve lives of workers and enhance enterprise success. A more transdisciplinary approach to research will be needed that integrates the skills of traditional and non-traditional OSH research disciplines, as well as broader research methods that support the transdisciplinary character of an expanded OSH paradigm. This article provides a summary of the presentations, discussion, and recommendations that will inform the agenda of the Expanded Focus for Occupational Safety and Health (Ex4OSH) International Conference, planned for December 2021.
Microbiological Reduction of Molybdenum to Molybdenum Blue as a Sustainable Remediation Tool for Molybdenum: A Comprehensive Review
Authors: Yakasai Hafeez Muhammad , Rahman Mohd Fadhil , Manogaran Motharasan , Yasid Nur Adeela , Syed Mohd Arif , Shamaan Nor Aripin , Shukor Mohd Yunus
Published: May 2021

Molybdenum (Mo) microbial bioreduction is a phenomenon that is beginning to be recognized globally as a tool for the remediation of molybdenum toxicity. Molybdenum toxicity continues to be demonstrated in many animal models of spermatogenesis and oogenesis, particularly those of ruminants. The phenomenon has been reported for more than 100 years without a clear understanding of the reduction mechanism, indicating a clear gap in the scientific knowledge. This knowledge is not just fundamentally important—it is specifically important in applications for bioremediation measures and the sustainable recovery of metal from industrial or mine effluent. To date, about 52 molybdenum-reducing bacteria have been isolated globally. An increasing number of reports have also been published regarding the assimilation of other xenobiotics. This phenomenon is likely to be observed in current and future events in which the remediation of xenobiotics requires microorganisms capable of degrading or transforming multi-xenobiotics. This review aimed to comprehensively catalogue all of the characterizations of molybdenum-reducing microorganisms to date and identify future opportunities and improvements.