bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Reported and Objective Impact of the COVID-19 Pandemic on Planning, Compliance and Timeliness of a Diagnostic Colonoscopy after a Positive FIT Screening Result in the Flemish Colorectal Cancer Screening Program
Authors: Hoeck Sarah , Janssens Sharon , Van Hal Guido , Van Herck Koen
Published: July 2022

Background: During the COVID-19 pandemic, the Flemish colorectal cancer (CRC) screening program (by fecal immunochemical test, FIT) was suspended and non-urgent medical procedures were discommended. This study estimates how this impacted diagnostic colonoscopy (DC) scheduling after a positive FIT and the interval between both in 2020. Methods: An online survey was sent to participants in the Flemish CRC screening program with a positive FIT but without a DC to explore the possible impact of COVID-19 on the scheduling of a DC. Self-reported survey results were complemented with objective data on DC compliance and the interval between FIT and DC. Results: In 2020, DC compliance was 4–5% lower than expected (for 3780 positive FITs no DC was performed). In February–March 2020, the median time between a positive FIT and DC significantly increased. Survey participants reported fear of COVID-19 contamination, perception to create hospital overload, delay in non-urgent medical procedures (on government advice) and not being sure a DC could be performed as contributing reasons. Conclusions: On top of a 3% lower participation, the COVID-19 pandemic further increased existing DC non-compliance and the positive FIT–DC interval. The survey confirmed the crucial role of COVID-19 in the decision not to plan a DC.
The Malay Literacy of Suicide Scale: A Rasch Model Validation and Its Correlation with Mental Health Literacy among Malaysian Parents, Caregivers and Teachers
Authors: Phoa Picholas Kian Ann , Razak Asrenee Ab , Kuay Hue San , Ghazali Anis Kausar , Rahman Azriani Ab , Husain Maruzairi , Bakar Raishan Shafini , Gani Firdaus Abdul
Published: July 2022

The 27-item Literacy of Suicide Scale (LOSS) is a test designed to measure the respondent’s suicide knowledge. The purpose of this study is to examine the psychometric properties of the Malay-translated version of the LOSS (M-LOSS) and its association to sociodemographic factors and mental health literacy. The 27-item LOSS was forward–backward translated into Malay, and the content and face validities were assessed. The version was distributed to 750 respondents across West Malaysia. Rasch model analysis was then conducted to assess the scale’s psychometric properties. The validated M-LOSS and the Malay version of the Mental Health Knowledge Schedule (MAKS-M) were then distributed to 867 respondents to evaluate their level of suicide literacy, mental health literacy, and their correlation. Upon Rasch analysis, 26 items were retained. The scale was found to be unidimensional, with generally satisfying separation and reliability indexes. Sex, socio-economic status, and experience in mental health were found to significantly impact the mean score for mental health literacy. This study also found a significant mean difference for suicide literacy across school types. Furthermore, while this study observed a weak but significant negative correlation between age and suicide literacy, no correlation was found between mental health and suicide literacy.
Mode of Delivery Is Associated with Postpartum Depression: Do Women with and without Depression History Exhibit a Difference?
Authors: Liu Tsai-Ching , Peng Hui-Chun , Chen Conmin , Chen Chin-Shyan
Published: July 2022

Whether the mode of birth delivery correlates with the incidence of postpartum depression (PPD) is still under debate. This study seeks to clarify such a correlation and to further investigate if there are any differences in the likelihood of PPD medical care use among women with and without a history of depression. A logistic regression at two assessment points (6-month and 12-month postpartum) on the National Health Insurance Database in Taiwan is performed. In total, 32,729 women were included who gave first birth from 2007 to 2011 via cesarean section (CS), elective CS, and vaginal delivery, of whom 3580 (10.9%) were diagnosed with a history of depression. Findings show that CS was associated with a higher likelihood of PPD doctor visits regardless of whether the women have a history of depression or not, but elective CS tended to have different impacts for these two groups of women. Mentally healthy women who experienced elective CS had 1.36- and 1.64-times higher risk of PDD medical care use than those who delivered vaginally, whereas previous depressive women undertaking an elective cesarean birth had no significant difference observed in incidences. A notably higher risk of elective CS delivery versus vaginal delivery for mentally healthy women suggests that elective CS is not clinically appropriate, yet it might be an alternative to vaginal delivery with careful counseling for pregnant women who experience a history of depression.
Improvement of In Vitro Seed Germination and Micropropagation of Amomum tsao-ko (Zingiberaceae Lindl.)
Authors: Khuat Quyet V. , Kalashnikova Elena A. , Kirakosyan Rima N. , Nguyen Hai T. , Baranova Ekaterina N. , Khaliluev Marat R.
Published: July 2022

Black cardamom (Amomum tsao-ko Crevost & Lemarié) is a spice plant of great commercial value in Vietnam, but with limited propagation ability. Its seeds are characterized by a thick and hard seed coat, a small endosperm, and a small embryo, which are the causes of the physical dormancy of the seeds and low germination. Attempts in this study to improve the germination rate and achieve uniform germination included mechanical scarification, immersion in hot or cold water, acid scarification, and the application of plant growth regulators. Although immersion of seeds in cold water and application of plant growth regulators (PGRs) (gibberellic acid (GA3) and 1-naphtaleneacetic acid (NAA)) showed positive effects on seed germination and subsequent seedling growth, mechanical scarification provided the highest germination rate of black cardamom seeds (68.0%) and significantly shortened germination time (53.7 days) compared to control (16.0% and 74.7 days). On the other hand, an efficient micropropagation protocol has been established using shoot tip explants derived from in-vitro-grown seedlings. Murashige and Skoog (MS) medium supplemented with 4.0 mg/L 6-benzylaminopurine (BAP) + 0.5 mg/L NAA proved to be most suitable for rapid multiplication and rooting, providing a mean of 5.4 shoots per explant, 6.8 cm shoot length, and 16.2 roots per explant after 7 weeks of culture. Well-rooted black cardamom plantlets have been successfully adapted to ex vitro conditions. “Fasco” bio-soil was more suitable for acclimatization, with a 48.9% survival rate, 23.3 cm plant length, and 5.7 leaves per plant after 3 months of planting. Improved germination and multiplication protocols can be used to improve propagation performances and to develop elite of black cardamom planting material.
Idiosyncratic Profile of Perceived Emotional Intelligence and Post-Traumatic Growth in Breast Cancer Survivors: Findings of a Multiple Mediation Model
Authors: Guil Rocío , Ruiz-González Paula , Morales-Sánchez Lucía , Gómez-Molinero Rocío , Gil-Olarte Paloma
Published: July 2022

Psycho-oncology research suggests that positive personal changes can occur after experiencing breast cancer. These changes can be understood as post-traumatic growth (PTG) and seem to be determined by emotional self-efficacy perception. This study aims to investigate the existence of different profiles of PTG and perceived emotional intelligence (PEI) among breast cancer survivors (BCSs) and healthy controls. Moreover, it aims to study the mechanisms through which PEI may mediate the relationship between disease survival and PTG. The total sample was 636 women divided into two groups: 56 BCS and 580 healthy controls who completed TMMS-24 and PTGI. The results displayed that BCSs apparently show a different profile of PTG and PEI compared to healthy women. The mediation analyses showed that survivorship explained 1.9% of PTG, increasing to 26.5% by the effect of PEI. An indirect effect showed that cancer survival predicts reduced levels of emotional attention, decreasing PTG. However, the most statistical indirect effect evidenced that BCSs regulate their emotions appropriately, having a powerful effect on PTG and counteracting the negative effects of poor emotional attention. Knowing the implications of PEI on PTG could improve follow-up from the time of diagnosis and supporting the patient to cope with the sequelae of the disease.
Occupational Exposure Assessment to Antineoplastic Drugs in Nine Italian Hospital Centers over a 5-Year Survey Program
Authors: Sottani Cristina , Grignani Elena , Cornacchia Marco , Negri Sara , Cuna Francesco , Cottica Danilo , Bruzzese Dario , Severi Paolo , Strocchi Daniele , Verna Giovanni , Leso Veruscka , Iavicoli Ivo
Published: July 2022

In the present study, surface contamination where antineoplastic drugs (ADs) are present was investigated, as occupational exposure risk is still an open debate. Despite recommendations and safety standard procedures being in place in health care settings, quantifiable levels of ADs are being reported in the recent literature. Thus, a survey monitoring program was conducted over five years (2016–2021) in nine Italian hospitals. The repeated surveys produced 8288 data points that have been grouped according to the main hospital settings, such as pharmacy areas and patient care units. Based on the most often prepared ADs, the investigated drugs were cyclophosphamide (CP), gemcitabine (GEM), 5-fluorouracil (5–FU), and platinum compounds (Pt). Patient care units had a frequency of positive wipe samples (59%) higher than pharmacies (44%). Conversely, pharmacies had a frequency of positive pad samples higher (24%) than patient care units (10%). Moreover, by statistical analysis, pad samples had a significantly higher risk of contamination in pharmacy areas than in patient care units. In this study, the 75th and the 90th percentiles of the contamination levels were obtained. The 90th percentile was chosen to describe a suitable benchmark that compares results obtained by the present research with those previously reported in the literature. Based upon surface contamination loads, our data showed that 5–FU had the highest concentration values, but the lowest frequency of positive samples. In pharmacy areas, the 90th percentile of 5–FU data distribution was less than 0.346 ng/cm2 and less than 0.443 ng/cm2 in patient care units. AD levels are higher than those reported for health care settings in other European countries yet trends of contamination in Italy have shown to decrease over time.
Characterization, Expression Profiling, and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants
Authors: Cui Weiti , Zhuang Zihan , Jiang Peihao , Pan Jincheng , Zhao Gan , Xu Sheng , Shen Wenbiao
Published: July 2022

Cinnamoyl-CoA reductase (CCR) is a pivotal enzyme in plant lignin synthesis, which has a role in plant secondary cell wall development and environmental stress defense. Alfalfa is a predominant legume forage with excellent quality, but the lignin content negatively affects fodder digestibility. Currently, there is limited information on CCR characteristics, gene expression, and its role in lignin metabolism in alfalfa. In this study, we identified 30 members in the CCR gene family of Medicago sativa. In addition, gene structure, conserved motif, and evolution analysis suggested MsCCR1–7 presumably functioned as CCR, while the 23 MsCCR-likes fell into three categories. The expression patterns of MsCCRs/MsCCR-likes suggested their role in plant development, response to environmental stresses, and phytohormone treatment. These results were consistent with the cis-elements in their promoters. Histochemical staining showed that lignin accumulation gradually deepened with the development, which was consistent with gene expression results. Furthermore, recombinant MsCCR1 and MsCCR-like1 were purified and the kinetic parameters were tested under four substrates. In addition, three-dimensional structure models of MsCCR1 and MsCCR-like1 proteins showed the difference in the substrate-binding motif H212(X)2K215R263. These results will be useful for further application for legume forage quality modification and biofuels industry engineering in the future.
The Effect of Phytochemicals and Food Bioactive Compounds on Diabetes
Authors: Yagasaki Kazumi , Muller Christo J. F.
Published: July 2022

There are three main types of diabetes, namely, type 1 diabetes, type 2 diabetes (T2D), and diabetes in pregnancy (gestational diabetes) [...]
Peeling Back the Layers of Lymph Gland Structure and Regulation
Authors: Kharrat Bayan , Csordás Gábor , Honti Viktor
Published: July 2022

During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche.
RETRACTED: Panoramic Manifold Projection (Panoramap) for Single-Cell Data Dimensionality Reduction and Visualization
Authors: Wang Yajuan , Xu Yongjie , Zang Zelin , Wu Lirong , Li Ziqing
Published: July 2022

Nonlinear dimensionality reduction (NLDR) methods such as t-Distributed Stochastic Neighbour Embedding (t-SNE) and Uniform Manifold Approximation and Projection (UMAP) have been widely used for biological data exploration, especially in single-cell analysis. However, the existing methods have drawbacks in preserving data’s geometric and topological structures. A high-dimensional data analysis method, called Panoramic manifold projection (Panoramap), was developed as an enhanced deep learning framework for structure-preserving NLDR. Panoramap enhances deep neural networks by using cross-layer geometry-preserving constraints. The constraints constitute the loss for deep manifold learning and serve as geometric regularizers for NLDR network training. Therefore, Panoramap has better performance in preserving global structures of the original data. Here, we apply Panoramap to single-cell datasets and show that Panoramap excels at delineating the cell type lineage/hierarchy and can reveal rare cell types. Panoramap can facilitate trajectory inference and has the potential to aid in the early diagnosis of tumors. Panoramap gives improved and more biologically plausible visualization and interpretation of single-cell data. Panoramap can be readily used in single-cell research domains and other research fields that involve high dimensional data analysis.
Glyoxal Fixation Is Optimal for Immunostaining of Brain Vessels, Pericytes and Blood-Brain Barrier Proteins
Authors: Thomas Sithara , Sadanandan Jayanarayanan , Blackburn Spiros L. , McBride Devin W. , Dienel Ari , Hong Sungha , Zeineddine Hussein A. , Thankamani Peeyush Kumar
Published: July 2022

Brain vascular staining is very important for understanding cerebrovascular pathologies. 4% paraformaldehyde is considered the gold standard fixation technique for immunohistochemistry and it revolutionized the examination of proteins in fixed tissues. However, this fixation technique produces inconsistent immunohistochemical staining results due to antigen masking. Here, we test a new fixation protocol using 3% glyoxal and demonstrate that this method improves the staining of the brain vasculature, pericytes, and tight junction proteins compared to 4% paraformaldehyde. Use of this new fixation technique will provide more detailed information about vascular protein expressions, their distributions, and colocalizations with other proteins at the molecular level in the brain vasculature.
Conditioned Medium of Human Pluripotent Stem Cell-Derived Neural Precursor Cells Exerts Neurorestorative Effects against Ischemic Stroke Model
Authors: Hur Hye-Jin , Lee Ji Yong , Kim Do-Hun , Cho Myung Soo , Lee Sangsik , Kim Han-Soo , Kim Dong-Wook
Published: July 2022

Previous studies have shown that early therapeutic events of neural precursor cells (NPCs) transplantation to animals with acute ischemic stroke readily protected neuronal cell damage and improved behavioral recovery through paracrine mechanisms. In this study, we tested the hypothesis that administration of conditioned medium from NPCs (NPC-CMs) could recapitulate the beneficial effects of cell transplantation. Rats with permanent middle cerebral artery occlusion (pMCAO) were randomly assigned to one of the following groups: PBS control, Vehicle (medium) controls, single (NPC-CM(S)) or multiple injections of NPC-CM(NPC-CM(M)) groups. A single intravenous injection of NPC-CM exhibited strong neuroregenerative potential to induce behavioral recovery, and multiple injections enhanced this activity further by suppressing inflammatory damage and inducing endogenous neurogenesis leading to histopathological and functional recovery. Proteome analysis of NPC-CM identified a number of proteins that are known to be associated with nervous system development, neurogenesis, and angiogenesis. In addition, transcriptome analysis revealed the importance of the inflammatory response during stroke recovery and some of the key hub genes in the interaction network were validated. Thus, our findings demonstrated that NPC-CM promoted functional recovery and reduced cerebral infarct and inflammation with enhanced endogenous neurogenesis, and the results highlighted the potency of NPC-CM in stroke therapy.
The Potential Role of miRNA-Regulated Autophagy in Alzheimer’s Disease
Authors: Zhang Hu , Liang Jiling , Chen Ning
Published: July 2022

As a neurodegenerative disease, Alzheimer’s disease (AD) shows a higher incidence during the aging process, mainly revealing the characteristics of a significant decrease in cognition, uncontrolled emotion, and reduced learning and memory capacity, even leading to death. In the prevention and treatment of AD, some pharmacological therapy has been applied in clinical practice. Unfortunately, there are still limited effective treatments for AD due to the absence of clear and defined targets. Currently, it is recognized that the leading causes of AD include amyloid-β peptide (Aβ) deposition, hyperphosphorylation of tau protein, neurofibrillary tangles, mitochondrial dysfunction, and inflammation. With in-depth mechanistic exploration, it has been found that these causes are highly correlated with the dysfunctional status of autophagy. Numerous experimental results have also confirmed that the development and progression of AD is accompanied by an abnormal functional status of autophagy; therefore, regulating the functional status of autophagy has become one of the important strategies for alleviating or arresting the progression of AD. With the increasing attention given to microRNAs (miRNAs), more and more studies have found that a series of miRNAs are involved in the development and progression of AD through the indirect regulation of autophagy. Therefore, regulating autophagy through targeting these miRNAs may be an essential breakthrough for the prevention and treatment of AD. This article summarizes the regulation of miRNAs in autophagy, with the aim of providing a new theoretical reference point for the prevention and treatment of AD through the indirect regulation of miRNA-mediated autophagy.
Transcriptome Analysis of Air Space-Type Variegation Formation in Trifolium pratense
Authors: Zhang Jianhang , Li Jiecheng , Zou Lu , Li Hongqing
Published: July 2022

Air space-type variegation is the most diverse among the species of known variegated leaf plants and is caused by conspicuous intercellular spaces between the epidermal and palisade cells and among the palisade cells at non-green areas. Trifolium pratense, a species in Fabaceae with V-shaped air space-type variegation, was selected to explore the application potential of variegated leaf plants and accumulate basic data on the molecular regulatory mechanism and evolutionary history of leaf variegation. We performed comparative transcriptome analysis on young and adult leaflets of variegated and green plants and identified 43 candidate genes related to air space-type variegation formation. Most of the genes were related to cell-wall structure modification (CESA, CSL, EXP, FLA, PG, PGIP, PLL, PME, RGP, SKS, and XTH family genes), followed by photosynthesis (LHCB subfamily, RBCS, GOX, and AGT family genes), redox (2OG and GSH family genes), and nitrogen metabolism (NodGS family genes). Other genes were related to photooxidation, protein interaction, and protease degradation systems. The downregulated expression of light-responsive LHCB subfamily genes and the upregulated expression of the genes involved in cell-wall structure modification were important conditions for air space-type variegation formation in T. pratense. The upregulated expression of the ubiquitin-protein ligase enzyme (E3)-related genes in the protease degradation systems were conducive to air space-type variegation formation. Because these family genes are necessary for plant growth and development, the mechanism of the leaf variegation formation in T. pratense might be a widely existing regulation in air space-type variegation in nature.
Role of Transposable Elements in Genome Stability: Implications for Health and Disease
Authors: Bhat Audesh , Ghatage Trupti , Bhan Sonali , Lahane Ganesh P. , Dhar Arti , Kumar Rakesh , Pandita Raj K. , Bhat Krishna M. , Ramos Kenneth S. , Pandita Tej K.
Published: July 2022

Most living organisms have in their genome a sizable proportion of DNA sequences capable of mobilization; these sequences are commonly referred to as transposons, transposable elements (TEs), or jumping genes. Although long thought to have no biological significance, advances in DNA sequencing and analytical technologies have enabled precise characterization of TEs and confirmed their ubiquitous presence across all forms of life. These findings have ignited intense debates over their biological significance. The available evidence now supports the notion that TEs exert major influence over many biological aspects of organismal life. Transposable elements contribute significantly to the evolution of the genome by giving rise to genetic variations in both active and passive modes. Due to their intrinsic nature of mobility within the genome, TEs primarily cause gene disruption and large-scale genomic alterations including inversions, deletions, and duplications. Besides genomic instability, growing evidence also points to many physiologically important functions of TEs, such as gene regulation through cis-acting control elements and modulation of the transcriptome through epigenetic control. In this review, we discuss the latest evidence demonstrating the impact of TEs on genome stability and the underling mechanisms, including those developed to mitigate the deleterious impact of TEs on genomic stability and human health. We have also highlighted the potential therapeutic application of TEs.
Unsteady Analysis of a Pulsating Alternate Flow Pattern in a Radial Vaned Diffuser
Authors: Poujol Nicolas , Buisson Martin , Duquesne Pierre , Trébinjac Isabelle
Published: July 2022

In centrifugal compressors, Mild Surge (MS) leads to unstable operation. Previous experimental work on a centrifugal compressor designed and built by Safran Helicopter Engines (SafranHE) showed that MS corresponds to the pulsation of an alternate stall pattern at the Helmholtz frequency of the test rig on two channels in the radial diffuser. The present contribution experimentally investigates the impact of the Inlet Guide Vane (IGV) stagger angle on this alternate flow and numerically studies the topology of this pulsating alternate flow. The experimental investigation is performed with unsteady pressure sensors, and shows that the IGV stagger angle only impacts the pulsation frequency of the alternate flow pattern. This change is explained by the dependence of the Helmholtz frequency on the compressor inlet section. The topological analysis of the average flow field, computed from wall-resolved Unsteady Reynolds-Averaged Navier–Stokes (URANS) simulations, demonstrates that the saddle point (major critical point) in the corner hub/suction side of the stalled blade migrates upstream while staying in the corner if the mass flow rate decreases. One main blade over two is stalled on both sides because the flow originating from this corner separation circumvents the trailing edge and migrates upstream along the pressure side. In the simulation, the pulsation of the alternate stall is coupled with the reflection of acoustic waves on the inlet and outlet planes, regarded as an environmental effect.
The Strategy of Factors Influencing Learning Satisfaction Explored by First and Second-Order Structural Equation Modeling (SEM)
Authors: Mia Mohammed Mamun , Zayed Nurul Mohammad , Islam Khan Mohammad Anwarul , Nitsenko Vitalii , Matusevych Tetiana , Mordous Iryna
Published: July 2022

The goal of this research was to create a partial least square structural equation model (PLS-SEM) with a second-order structural model to investigate the interaction between research-based methodologies and relationship factors that significantly influence learning satisfaction among university students. The instruments used in this study were a simple random sampling technique for structural equation model (SEM) analysis, while a quantitative process of survey data collection was manipulated through SPSS and Smart-PLS. The presented study attempted to explore whether teachers’ strategies are linked with their students for the students’ learning satisfaction. Thus, it represents the demands and expectations of two statistically significant common phenomena: research-based components and relationship approach components. This set of teaching techniques encourages university students and enhances their learning satisfaction. Moreover, this study explored teaching strategies that influence factors having a directly significant influence on learning satisfaction at university level. Each factor measures the relationship’s construct, proven to be a second-order SEM reflective model that is statistically significant. Our study explored learning satisfaction as an integral part of teaching strategies, by first- and second-order structural equation modeling, supported by students’ expectations, and the study’s empirical results provide potential implications for learning satisfaction.
Sodium-Glucose Cotransporter-2 Inhibitors-from the Treatment of Diabetes to Therapy of Chronic Heart Failure
Authors: Kurczyński Dominik , Hudzik Bartosz , Jagosz Marta , Zabierowski Jan , Nowak Jolanta , Tomasik Andrzej , Badziński Arkadiusz , Rozentryt Piotr , Gąsior Mariusz
Published: July 2022

Sodium-glucose cotransporter-2 (SGLT2) inhibitors are currently the second-line pharmacotherapy in type 2 diabetes, particularly through their effectiveness in reducing glycemia, but also due to their cardioprotective and nephroprotective effects. In light of surprisingly satisfactory results from large, randomized trials on gliflozins, SGLT2 received the highest recommendation (Class IA) with the highest level of evidence (A) in the treatment algorithm for HF with reduced LVEF in recent ESC HF guidelines. This great breakthrough in the treatment of HF is due to different mechanisms of action of gliflozins that are reported to be able to change the natural course of HF by reducing the risk of both hospitalization and death. They are recommended regardless of the patient’s diabetes status. This review summarizes the up-to-date literature on their beneficial and pleiotropic impact on the cardiovascular system.
Acetaminophen Disrupts the Development of Pharyngeal Arch-Derived Cartilage and Muscle in Zebrafish
Authors: Glasco Derrick M. , Wang Zhidong , Kang Seonwoo , Funkhouser Avery T.
Published: July 2022

Acetaminophen is a common analgesic, but its potential effects on early embryonic development are not well understood. Previous studies using zebrafish (Danio rerio) have described the effects of acetaminophen on liver development and physiology, and a few have described gross physiological and morphological defects. Using a high but non-embryonic lethal dose of acetaminophen, we probed for defects in zebrafish craniofacial cartilage development. Strikingly, acetaminophen treatment caused severe craniofacial cartilage defects, primarily affecting both the presence and morphology of pharyngeal arch-derived cartilages of the viscerocranium. Delaying acetaminophen treatment restored developing cartilages in an order correlated with their corresponding pharyngeal arches, suggesting that acetaminophen may target pharyngeal arch development. Craniofacial cartilages are derived from cranial neural crest cells; however, many neural crest cells were still seen along their expected migration paths, and most remaining cartilage precursors expressed the neural crest markers sox9a and sox10, then eventually col2a1 (type II collagen). Therefore, the defects are not primarily due to an early breakdown of neural crest or cartilage differentiation. Instead, apoptosis is increased around the developing pharyngeal arches prior to chondrogenesis, further suggesting that acetaminophen may target pharyngeal arch development. Many craniofacial muscles, which develop in close proximity to the affected cartilages, were also absent in treated larvae. Taken together, these results suggest that high amounts of acetaminophen can disrupt multiple aspects of craniofacial development in zebrafish.
Efficiency of Priority Queue Architectures in FPGA
Authors: Kohútka Lukáš
Published: July 2022

This paper presents a novel SRAM-based architecture of a data structure that represents a set of multiple priority queues that can be implemented in FPGA or ASIC. The proposed architecture is based on shift registers, systolic arrays and SRAM memories. Such architecture, called MultiQueue, is optimized for minimum chip area costs, which leads to lower energy consumption too. The MultiQueue architecture has constant time complexity, constant critical path length and constant latency. Therefore, it is highly predictable and very suitable for real-time systems too. The proposed architecture was verified using a simplified version of UVM and applying millions of instructions with randomly generated input values. Achieved FPGA synthesis results are presented and discussed. These results show significant savings in FPGA Look-Up Tables consumption in comparison to existing solutions. More than 63% of Look-Up Tables can be saved using the MultiQueue architecture instead of the existing priority queues.
A Manta Ray Robot with Soft Material Based Flapping Wing
Authors: Liu Qimeng , Chen Hao , Wang Zhenhua , He Qu , Chen Linke , Li Weikun , Li Ruipeng , Cui Weicheng
Published: July 2022

Recent research on robotic fish mainly focused on the bionic structure design and realizing the movement with smart materials. Although many robotic fish have been proposed, most of these works were oriented toward shallow water environments and are mostly built with purely rigid structures, limiting the mobility and practical usability of robotic fish. Inspired by the stability of the real manta ray, a manta ray robot design is proposed with soft material made flapping wing based on an open-source ROV (Remotely Operated Vehicle). The flapping wing structure with three different materials mimics the wide pectoral fins of real manta rays, which have bones, muscles, and skin. Furthermore, its modular design makes it easy to install and disassemble. The kinematic and hydrodynamic analysis of the manta ray robot are simulated in this paper. The actual manta ray robot is fabricated and several sets of test are performed in the pool. The robot can swim forward continually and stably with a simple rolling and pitching pattern.
A Lightweight Sea Surface Object Detection Network for Unmanned Surface Vehicles
Authors: Yang Zhangqi , Li Ye , Wang Bo , Ding Shuoshuo , Jiang Peng
Published: July 2022

For unmanned surface vehicles (USVs), perception and control are commonly performed in embedded devices with limited computing power. Sea surface object detection can provide sufficient information for USVs, while most algorithms have poor real-time performance on embedded devices. To achieve real-time object detection on the USV platform, this paper designs a lightweight object detection network based on YOLO v5. In our work, an improved ShuffleNet v2 based on the attention mechanism was adopted as a backbone network to extract features. The depth-wise separable convolution module was introduced to rebuild the neck network. Additionally, the fusion method was changed from Concat to Add to optimize the feature fusion module. Experiments show that the proposed method reached 32.64 frames per second (FPS) on the Nvidia Jetson AGX Xavier and achieved a mean average precision (mAP) of 93.1% and 93.9% on our dataset and Singapore Maritime Dataset, respectively. Moreover, the number of model parameters of the proposed network was only 25% of that of YOLO v5n. The proposed network achieves a better balance between speed and accuracy, which is more suitable for detecting sea surface objects for USVs.
Mitigation of Ice-Induced Vibration of Offshore Platform Based on Gated Recurrent Neural Network
Authors: Zhang Peng , Wu Zhihao , Cui Chunyi , Yao Ruqing
Published: July 2022

Ice-induced vibration is one of the major risks that face the offshore platform located in cold regions. In this paper, the gated recurrent neural network (GRNN) is utilized to predict and suppress the response of offshore platforms subjected to ice load. First, a simplified model of the offshore platform is derived and validated based on the finite element model (FEM). The time history of the floating ice load is generated using the harmonic superposition method. Gated Recurrent Unit Network (GRU) and the Long-Short-Term Memory Network (LSTM) are composed in MATLAB to predict the behavior of the off-shore platform. Afterward, the linear quadratic regulator (LQR) control algorithm is used to calculate the controlling force for the training of the GRU/LSTM-based prediction controller. Numerical results show that the ice-induced vibration response prediction method based on GRU network design can predict the structural response with satisfying accuracy, and the ice-induced vibration response control method based on the LSTM network and GRU network design can learn the LQR method well and achieve good control effect. Time lag and other problems that the vibration control programs often encountered were solved well.
Local Defogging Algorithm for the First Frame Image of Unmanned Surface Vehicles Based on a Radar-Photoelectric System
Authors: Yu Qingze , Su Yumin
Published: July 2022

Unmanned surface vehicles frequently encounter foggy weather when performing surface object tracking tasks, resulting in low optical image quality and object recognition accuracy. Traditional defogging algorithms are time consuming and do not meet real-time requirements. In addition, there are problems with oversaturated colors, low brightness, and overexposed areas in the sky. In order to solve the problems mentioned above, this paper proposes a defogging algorithm for the first frame image of unmanned surface vehicles based on a radar-photoelectric system. The algorithm involves the following steps. The first is the fog detection algorithm for sea surface image, which determines the presence of fog. The second is the sea-sky line extraction algorithm which realizes the extraction of the sea-sky line in the first frame image. The third is the object detection algorithm based on the sea-sky line, which extracts the target area near the sea-sky line. The fourth is the local defogging algorithm, which defogs the extracted area to obtain higher quality images. This paper effectively solves the problems above in the sea test and dramatically reduces the calculation time of the defogging algorithm by 86.7%, compared with the dark channel prior algorithm.
Selenium Combined with Methyl Jasmonate to Control Tomato Gray Mold by Optimizing Microbial Community Structure in Plants
Authors: Li Changyin , Hu Chengxiao , Xie Jiatao , Shi Guangyu , Wang Xu , Yuan Xiang , Li Keyi , Chen Siqi , Zhao Xiaohu , Fan Guocheng
Published: July 2022

Tomato cultivation is seriously affected by infection from Botrytis cinerea. The safe and effective control of tomato gray mold remains elusive. Plant-related microbial communities regulate not only plant metabolism but also plant immune systems. In this study, we observed that Selenium application in soil combined with foliar spraying of methyl jasmonate could reduce Botrytis cinerea infection in tomato fruits and leaves and improve tomato fruit quality. The infection rate of leaves decreased from 42.19% to 25.00%, and the vitamin C content increased by 22.14%. The bacterial community structure of the tomato was studied by using amplicon sequencing technology. The leaf bacterial alpha diversity of tomatoes treated with Se plus methyl jasmonate was significantly higher than that of the control. Then we isolated five strains antagonistic to Botrytis cinerea in vitro from tomato leaves in the treatment of Se plus methyl jasmonate. The antagonistic strains were identified as Bacillus subtilis and Bacillus velezensis. Spraying mixed antagonistic strain suspension significantly inhibited the diameter of Botrytis cinerea with an inhibition rate of 40.99%. This study revealed the key role of plant-beneficial bacteria recruited by Se combined with methyl jasmonate in improving tomato plant disease resistance. These findings may benefit our understanding of the new regulation of microorganisms on Botrytis cinerea.
Sustainable Agricultural Development Models of the Ecologically Vulnerable Karst Areas in Southeast Yunnan from the Perspective of Human–Earth Areal System
Authors: Zhao Xiaoqing , Xu Yifei , Wang Qian , Pu Junwei , Shi Xiaoqian , Huang Pei , Gu Zexian
Published: July 2022

Rocky desertification in ecologically-fragile karst areas limit regional socio-economic development in the face of significant human–earth conflict. Coordination of ecological restoration and agricultural development is critical for sustainable development in karst areas. From the perspective of the human–earth areal system, the framework of sustainable agricultural development was proposed in typically karst areas. We integrated principles of ecological vulnerability, resource and environmental carrying capacity, agricultural foundation, suitability of agricultural land, and the farmers’ willingness. In this study, we found the ecological vulnerability of Guangnan County was slight, but the proportion of moderate and severe vulnerability areas was high, with significant differences between the two sides of the line “Zhe (Zhetu)-Lian (Liancheng)-Yang (Yang Liu-jing)-Ban (Banbang)”. Then, we divided Guangnan County into three ecologically vulnerable zones. Following that, we proposed sustainable agricultural models for various zones. In slightly to mildly vulnerable zones, we propose constructing economic–ecological agricultural models, including woody oil, plateau characteristic fruiting forest, ecological tea plantations, suburban agriculture, and cultural–ecological tourism. In moderately to severely vulnerable zones, we recommend developing a stereoscopic agriculture model that combines planting and breeding, vegetation restoration, and herbivorous animal husbandry. In extremely vulnerable zones, we suggest constructing an ecologically natural restoration model and an agricultural ecological–tourism model. Our research provides references for ecological restoration, agricultural development, poverty alleviation consolidation, and rural revitalization in ecologically vulnerable karst areas of southeast Yunnan and similar regions.
Fabrication and Characterization of a Composite Ni-SDC Fuel Cell Cathode Reinforced by Ni Foam
Authors: Komorowska Gabriela , Wejrzanowski Tomasz , Jamroz Jan , Jastrzębska Agnieszka , Wróbel Wojciech , Tsai Shu-Yi , Fung Kuan-Zong
Published: July 2022

High-temperature fuel cells (namely, molten carbonate and solid oxide; MCFCs and SOFCs) require the cathode to be designed to maximize oxygen catalytic reduction, oxygen ion transport, electrical conductivity, and gas transport. This then leads to the optimization of the volume fraction and morphology of phases, as they are a pathway for electrons, ions, and gases to be continuous and self-interpenetrating. Apart from the functional properties, the cathode must be mechanically stable to prevent cracking during fuel cell assembly and operation. The manufacturing process of the composite cathode was optimized to meet such requirements in this research work. The tape casting technique and further firing process were used to fabricate the cathodes. The slurry for the green tape was composed of nickel (Ni), cerium oxide doped with samarium oxide (SDC), water (solvent), and an organic binder (which becomes pore space after firing). Each of these elements is necessary for the effective transport of specific species: electrons, oxygen, ions, and gas particles, respectively. Moreover, the nickel foam was embedded into the powder-based structure to improve mechanical strength. The study involved many technological issues, such as the effect of the SDC fraction on the cathode microstructure, mechanical strength, and chemical stability at high temperatures, and also involved environmental issues.
Structural Formation of Soil Concretes Based on Loam and Fly Ash, Modified with a Stabilizing Polymer Additive
Authors: Konovalova Nataliya , Pankov Pavel , Petukhov Valery , Fediuk Roman , Amran Mugahed , Vatin Nikolai
Published: July 2022

Finding new ways of recycling production waste to improve the characteristics of various building materials is an urgent scientific task. This article substantiates the possibility of the disposal of fly ash in the composition of soil concrete, which is used in the construction of the structural layers of road pavements, foundations of buildings and structures, as well as sites for various purposes. The scientific novelty lies in the fact that the structure formation of soil concretes based on loam and fly ash and modified with a stabilizing additive is being studied for the first time. It was found that the investigated fly ash, according to its hydraulic properties, is classified as latent active and can be introduced into the compositions of road soil concrete modified with additives of various resources. The effectiveness of the complex method of stabilization, due to changes in soil properties as a result of the use of the binding and stabilizing additives of polymer nature “Kriogelit”, is shown. It was found that the optimal content of binder and fly ash in the samples was 8 and 10 wt.%, respectively. It was established that the use of the stabilizing additive “Kriogelit” makes it possible to obtain soil concrete with the highest strength (compressive strength 2.5 MPa, flexural strength 0.5 MPa) and frost resistance of at least F15. The microstructure, the degree of dehydration and carbonization, and the phase composition of the initial raw mixtures and soil concretes stabilized with the addition of “Kriogelit” were studied by methods of scanning electron microscopy, X-ray diffraction analysis, differential scanning calorimetry, thermogravimetry, and infrared spectroscopy. It was shown that organo-mineral complexes, with the participation of polymer and montmorillonite, are formed in stabilized soil concrete. It was revealed that structure formation is accompanied by the physical adsorption of the polymer on active centers of silicate minerals, carbonization, and hydration–dehydration processes. It was found that the reason for the increase in the strength of stabilized soil concretes is the hydrophobization of the porous structure of minerals, as well as the formation of calcium oxide silicate and dicalcium hydrated silicate. By the method of performing biotests with the test objects Daphnia magna Straus and Chlorella vulgaris Beijer, it was proven that the developed road concretes modified with the stabilizing additive “Kriogelit” do not have an acute toxic effect on the test objects and are safe for the environment and human health.
Research on Monocrystalline Silicon Micro-Nano Structures Irradiated by Femtosecond Laser
Authors: Liu Yanan , Ding Ye , Xie Jichang , Chen Mingjun , Yang Lijun , Lv Xun , Yuan Julong
Published: July 2022

Femtosecond (fs) laser processing has received great attention for preparing novel micro-nano structures and functional materials. However, the induction mechanism of the micro-nano structures induced by fs lasers still needs to be explored. In this work, the laser-induced periodic surface structure (LIPSS) of monocrystalline silicon (Si) under fs laser irradiation is investigated. Three different layers named amorphous silicon (a-Si) layer, transition layer, and unaffected Si layer are observed after laser irradiation. The a-Si layer on the surface is generated by the resolidification of melting materials. The unaffected Si layer is not affected by laser irradiation and maintains the initial atomic structure. The transition layer consisting of a-Si and unaffected Si layers was observed under the irradiated subsurface. The phase transition mechanism of Si irradiated by fs laser is “amorphous transition”, with the absence of other crystal structures. A numerical model is established to describe the fs laser-Si interaction to characterize the electronic (lattice) dynamics of the LIPSS formation. The obtained results contribute to the understanding of fs laser processing of Si at the atomic scale as well as broaden the application prospects of fs laser for treating other semiconductor materials.