bluuebunny/crossref_metadata_2025 huggingface dataset:
Can a Selective PPARγ Modulator Improve Glycemic Control in Patients With Type 2 Diabetes With Fewer Side Effects Compared With Pioglitazone?
Authors: DePaoli Alex M. , Higgins Linda S. , Henry Robert R. , Mantzoros Christos , Dunn Fredrick L.
Published: April 2014

OBJECTIVE

INT131 besylate is a potent, nonthiazolidinedione, selective peroxisome proliferator–activated receptor γ (PPARγ) modulator (SPPARM) designed to improve glucose metabolism while minimizing the side effects of full PPARγ agonists. This placebo-controlled study compared the efficacy and side effects of INT131 besylate versus 45 mg pioglitazone HCl in subjects with type 2 diabetes (T2D).

RESEARCH DESIGN AND METHODS

This was a 24-week randomized, double-blind, placebo- and active-controlled study of 0.5–3.0 mg INT131 versus 45 mg pioglitazone or placebo daily in 367 subjects with T2D on sulfonylurea or sulfonylurea plus metformin. The primary efficacy analysis was the comparison of change from baseline to week 24 in hemoglobin A1c (HbA1c) across treatment groups. Fluid status was assessed with a prospective scoring system for lower-extremity pitting edema.

RESULTS

INT131 had a steep dose response for efficacy as measured by changes in HbA1c. After 24 weeks’ treatment, the 0.5-mg dose demonstrated minimal efficacy (HbA1c −0.3 ± 0.12%) and the 2-mg dose demonstrated near-maximal efficacy (HbA1c −1.1 ± 0.12%), which was not statistically different from the efficacy of 45 mg pioglitazone (HbA1c −0.9 ± 0.12%; P < 0.01 for noninferiority). With the 1-mg dose, INT131 provided significant improvements in glycemic control (HbA1c 0.8 ± 0.12; P < 0.001 vs. placebo) but with less edema, weight gain, and hemodilution than observed with 45 mg pioglitazone.

CONCLUSIONS

INT131 demonstrated dose-dependent reductions in HbA1c, equivalent to 45 mg pioglitazone, but with less fluid accumulation and weight gain, consistent with its SPPARM design.
EVALUATION OF SPECTRAL AND PROSODIC FEATURES OF SPEECH AFFECTED BY ORTHODONTIC APPLIANCES USING THE GMM CLASSIFIER
Authors: Přibil Jiří , Přibilová Anna , Ďuračkoá Daniela
Published: April 2014

The paper describes our experiment with using the Gaussian mixture models (GMM) for classification of speech uttered by a person wearing orthodontic appliances. For the GMM classification, the input feature vectors comprise the basic and the complementary spectral properties as well as the supra-segmental parameters. Dependence of classification correctness on the number of the parameters in the input feature vector and on the computation complexity is also evaluated. In addition, an influence of the initial setting of the parameters for GMM training process was analyzed. Obtained recognition results are compared visually in the form of graphs as well as numerically in the form of tables and confusion matrices for tested sentences uttered using three configurations of orthodontic appliances.
CONTINUOUS FIREFLY ALGORITHM FOR OPTIMAL TUNING OF PID CONTROLLER IN AVR SYSTEM
Authors: Bendjeghaba Omar
Published: April 2014

This paper presents a tuning approach based on Continuous firefly algorithm (CFA) to obtain the proportional-integral- derivative (PID) controller parameters in Automatic Voltage Regulator system (AVR). In the tuning processes the CFA is iterated to reach the optimal or the near optimal of PID controller parameters when the main goal is to improve the AVR step response characteristics. Conducted simulations show the effectiveness and the efficiency of the proposed approach. Furthermore the proposed approach can improve the dynamic of the AVR system. Compared with particle swarm optimization (PSO), the new CFA tuning method has better control system performance in terms of time domain specifications and set-point tracking.
Morocco and Its Women’s Rights Struggle
Authors: Elliott Katja Žvan
Published: April 2014

This article examines the rarely talked about subtleties of Moroccan reform in the realm of women’s rights and its inadequate fulfillment of obligations to international human rights standards. The Preamble to Morocco’s post-Arab Spring 2011 constitution follows the example of its 1996 version, in which the state declared its “determination to abide by the universally recognised human rights.” However, while the state is often hailed in the international forums and media as a true trendsetter in the realm of women’s rights in the Middle East and North Africa region, this analysis of the much celebrated Family Code and its two main goals-“doing justice to women” and “preserving men’s dignity”-and of the regime’s ambivalent discourse on gender equality as defined by the Convention on the Elimination of All Forms of Discrimination against Women (CEDAW) paints a more realistic picture. Both of these cases indicate that the state is failing to ameliorate the legal position of women and to consider women as autonomous and individual human beings with intrinsic rights not contingent upon first fulfilling their customary obligations. I contend, therefore, that the way the reformed Family Code has formulated its goals and the way that the law and the state continue to conceptualize a woman go against the main principle of individuality contained in the Universal Declaration of Human Rights and CEDAW to which Morocco has continually committed itself, at least on paper.
Religious Flexibilies of Older Yemeni Women in Sanaa
Authors: Pandya Sophia
Published: April 2014

This paper looks at the ways in which older generations of Yemeni women in Sanaa negotiate religious change. Practices that are associated with Sufism and popular folk Islam are prevalent in Yemen, especially among older, illiterate women who have had little to no access to textual, “orthodox” Islam. The past few decades in Sanaa, however, have witnessed a rise in socially-restrictive forms of Salafi Islam, especially among the younger generations, which has had consequences for Yemeni women and their ability to carry out roles in the public sector. On the other hand, the Salafis provide Qur’anic school education, including literacy training, for older, often illiterate, women for whom there are few other educational opportunities. The Salafis disapprove of Sufism and folk Islam, and in Yemen these activities have often been forced underground. Here I analyze the types of benefits older Yemeni women receive from their differing religious practices and the ways in which they accommodate doctrinal contradiction.
Evaluation of Antifungal Activity of Botanicals for Postharvest Management of Mango Anthracnose (Colletotrichum gloeosporioides)
Authors: Alemu Kumlachew , Ayalew Amare , Weldetsadik Kebede
Published: April 2014

Antifungal activities of 20 plant species were tested against Colletotrichum gloeosporioides, the causal agent of mango anthracnose. Culture sensitivity tests of the plant extracts were done using paper disc diffusion inhibition method. Among the twenty plant species extracted with ethyl acetate Ruta chalepensis and Eucalyptus globulus showed the highest activity. Methanol extracts of three plants, namely Datura stramonium, Adhatoda schimperiana and Eucalyptus globulus also showed remarkable activity. The extracts also significantly inhibit conidial germination of the test fungi over the control. Plant species that showed potential in vitro were selected and aqueous extracts of the plants were evaluated on artificially and naturally infected mango fruits. Development of anthracnose on mango fruits was significantly affected on both tests. Results of this study indicate that use of botanical for the control of anthracnose disease in mango is less expensive, safe and could serve as a good alternative to synthetic fungicides.DOI: http://dx.doi.org/10.3126/ijls.v8i1.9957
The behavior of residual tumors and facial nerve outcomes after incomplete excision of vestibular schwannomas
Authors: Chen Zhengnong , Prasad Sampath Chandra , Di Lella Filippo , Medina Marimar , Piccirillo Enrico , Taibah Abdelkader , Russo Alessandra , Yin Shankai , Sanna Mario
Published: April 2014

Object

The authors evaluated the behavior of residual tumors and facial nerve outcomes after incomplete excision of vestibular schwannomas (VSs).

Methods

The case records of all patients who underwent surgical treatment of VSs were analyzed. All patients in whom an incomplete excision had been performed were analyzed. Incomplete excision was defined as near-total resection (NTR), subtotal resection (STR), and partial resection (PR). Tumors in the NTR and STR categories were followed up with a wait-and-rescan approach, whereas the tumors in the PR category were subjected to a second-stage surgery and were excluded from this series. All patients included in the study underwent baseline MRI at the 3rd and 12th postoperative months, and repeat imaging was subsequently performed every year for 7–10 years postoperatively or as indicated clinically. Preoperative and postoperative facial function was noted.

Results

Of the 2368 patients who underwent surgery for VS, 111 patients who had incomplete excisions of VSs were included in the study. Of these patients, 73 (65.77%) had undergone NTR and 38 (34.23%) had undergone STR. Of the VSs, 62 (55.86%) were cystic and 44 (70.97%) of these cystic VSs underwent NTR. The residual tumor was left behind on the facial nerve alone in 62 patients (55.86%), on the facial nerve and vessels in 2 patients (1.80%), on the facial nerve and brainstem in 15 patients (13.51%), and on the brainstem alone in 25 patients (22.52%). In the 105 patients with normal preoperative facial nerve function, postoperative facial nerve function was House-Brackmann (HB) Grades I and II in 51 patients (48.57%), HB Grade III in 34 patients (32.38%), and HB Grades IV–VI in 20 patients (19.05%). Seven patients (6.3%) showed evidence of tumor regrowth on follow-up MRI. All 7 patients (100%) who showed evidence of tumor regrowth had undergone STR. No patient in the NTR group exhibited regrowth. The Kaplan-Meier plot demonstrated a 5-year tumor regrowth-free survival of 92%, with a mean disease-free interval of 140 months (95% CI 127–151 months). The follow-up period ranged from 12 to 156 months (mean 45.4 months).

Conclusions

The authors' report and review of the literature show that there is undoubtedly merit for NTR and STR for preservation of the facial nerve. On the basis of this they propose an algorithm for the management of incomplete VS excisions. Patients who undergo incomplete excisions must be subjected to follow-up MRI for a period of at least 7–10 years. When compared with STR, NTR via an enlarged translabyrinthine approach has shown to have a lower rate of regrowth of residual tumor, while having almost the same result in terms of facial nerve function.
Challenges in sciatic nerve repair: anatomical considerations
Authors: Burks S. Shelby , Levi David J. , Hayes Seth , Levi Allan D.
Published: April 2014

Object

The object of this study was to highlight the challenge of insufficient donor graft material in peripheral nerve surgery, with a specific focus on sciatic nerve transection requiring autologous sural nerve graft.

Methods

The authors performed an anatomical analysis of cadaveric sciatic and sural nerve tissue. To complement this they also present 3 illustrative clinical cases of sciatic nerve injuries with segmental defects. In the anatomical study, the cross-sectional area (CSA), circumference, diameter, percentage of neural tissue, fat content of the sural nerves, as well as the number of fascicles, were measured from cadaveric samples. The percentage of neural tissue was defined as the CSA of fascicles lined by perineurium relative to the CSA of the sural nerve surrounded by epineurium.

Results

Sural nerve samples were obtained from 8 cadaveric specimens. Mean values and standard deviations from sural nerve measurements were as follows: CSA 2.84 ± 0.91 mm2, circumference 6.67 ± 1.60 mm, diameter 2.36 ± 0.43 mm, fat content 0.83 ± 0.91 mm2, and number of fascicles 9.88 ± 3.68. The percentage of neural tissue seen on sural nerve cross-section was 33.17% ± 4.96%. One sciatic nerve was also evaluated. It had a CSA of 37.50 mm2, with 56% of the CSA representing nerve material. The estimated length of sciatic nerve that could be repaired with a bilateral sural nerve harvest (85 cm) varied from as little as 2.5 cm to as much as 8 cm.

Conclusions

Multiple methods have been used in the past to repair sciatic nerve injury but most commonly, when a considerable gap is present, autologous nerve grafting is required, with sural nerve being the foremost source. As evidenced by the anatomical data reported in this study, a considerable degree of variability exists in the diameter of sural nerve harvests. Conversely, the percentage of neural tissue is relatively consistent across specimens. The authors recommend that the peripheral nerve surgeon take these points into consideration during nerve grafting as insufficient graft material may preclude successful recovery.
Clinical course of vestibular schwannoma in pediatric neurofibromatosis Type 2
Authors: Choi Jung Won , Lee Ji Yeoun , Phi Ji Hoon , Wang Kyu-Chang , Chung Hyun-Tai , Paek Sun Ha , Kim Dong Gyu , Park Sung-Hye , Kim Seung-Ki
Published: April 2014

Object

Neurofibromatosis Type 2 (NF2) is an autosomal-dominant inherited disease, characterized by multiple neoplasia syndromes, including meningioma, schwannoma, glioma, and ependymoma. In this report, the authors present their clinical experience with pediatric NF2 patients. In particular, they focused on the clinical course of vestibular schwannoma (VS), including the natural growth rate, tumor control, and functional hearing outcomes.

Methods

From May 1988 to June 2012, the authors recruited patients who were younger than 18 years and fulfilled the Manchester criteria. In total, 25 patients were enrolled in this study. The authors analyzed the clinical course of these patients. In addition, they measured the natural growth rate of VS before any treatment in these children with NF2. Then, they evaluated the tumor control rate and functional hearing outcomes after the treatment of VS.

Results

The mean age at the onset of NF2-related symptoms was 9.9 ± 4.5 years (mean ± SD, range 1–17 years). The mean age at the diagnosis of NF2 was 12.9 ± 2.9 years (range 5–17 years). The mean follow-up period was 89.3 months (range 12–311 months). As initial manifestations, nonvestibular symptoms were frequently observed in pediatric patients with NF2. The mean natural growth rate of VS was 0.33 ± 0.41 cm3/year (range 0–1.35 cm3/year). The tumor control rate of VS was 35.3% at 3 years after Gamma Knife surgery (GKS). The actuarial rate of useful hearing preservation was 67% in the 1st year and 53% in the 5th year after GKS.

Conclusions

Clinical manifestations in children with NF2 were highly variable, compared with their adult counterparts. The natural growth rate of VS in children is slow, and this oncological feature may explain the diverse clinical manifestations besides vestibular symptoms in children with NF2. The treatment outcome of GKS for VS in children with NF2 was not favorable compared with previous reports of affected adults.
External validation of the Chicago Chiari Outcome Scale
Authors: Yarbrough Chester K. , Greenberg Jacob K. , Smyth Matthew D. , Leonard Jeffrey R. , Park Tae Sung , Limbrick David D.
Published: April 2014

Object

Historically, assessment of clinical outcomes following surgical management of Chiari malformation Type I (CM-I) has been challenging due to the lack of a validated instrument for widespread use. The Chicago Chiari Outcome Scale (CCOS) is a novel system intended to provide a less subjective evaluation of outcomes for patients with CM-I. The goal of this study was to externally validate the performance of the CCOS.

Methods

Patients undergoing surgery for CM-I between 2001 and 2012 were reviewed (n = 292). Inclusion criteria for this study were as follows: 1) patients receiving primary posterior fossa decompression; 2) at least 5.5 months of postoperative clinical follow-up; and 3) patients ≤ 18 years of age at the time of surgery. Outcomes were evaluated using the CCOS, along with a “gestalt” impression of whether patients experienced significant improvement after surgery. A subgroup of 118 consecutive patients undergoing operations between 2008 and 2010 was selected for analysis of interrater reliability (n = 73 meeting inclusion/exclusion criteria). In this subgroup, gestalt and CCOS scores were independently determined by 2 reviewers, and interrater reliability was assessed using the intraclass correlation coefficient (ICC) and kappa (κ) statistic.

Results

The median CCOS score was 14, and 67% of patients had improved gestalt scores after surgery. Overall, the CCOS was effective at identifying patients with improved outcome after surgery (area under curve = 0.951). The interrater reliability of the CCOS (ICC = 0.71) was high, although the reliability of the component scores ranged from poor to good (ICC 0.23–0.89). The functionality subscore demonstrated a low ICC and did not add to the predictive ability of the logistic regression model (likelihood ratio = 1.8, p = 0.18). When analyzing gestalt outcome, there was moderate agreement between raters (κ = 0.56).

Conclusions

In this external validation study, the CCOS was effective at identifying patients with improved outcomes and proved more reliable than the authors' gestalt impression of outcome. However, certain component subscores (functionality and nonpain symptoms) were found to be less reliable, and may benefit from further definition in score assignment. In particular, the functionality subscore does not add to the predictive ability of the CCOS, and may be unnecessary. Overall, the authors found the CCOS to be an improvement over the previously used assessment of outcome at their institution.
Predictors of unplanned readmission in patients undergoing lumbar decompression: multi-institutional analysis of 7016 patients
Authors: Kim Bobby D. , Smith Timothy R. , Lim Seokchun , Cybulski George R. , Kim John Y. S.
Published: April 2014

Object

Unplanned hospital readmission represents a large financial burden on the Centers for Medicare and Medicaid Services, commercial insurance payers, hospitals, and individual patients, and is a principal target for cost reduction. A large-scale, multi-institutional study that evaluates risk factors for readmission has not been previously performed in patients undergoing lumbar decompression procedures. The goal of this multicenter retrospective study was to find preoperative, intraoperative, and postoperative predictive factors that result in unplanned readmission (UR) after lumbar decompression surgery.

Methods

The National Surgical Quality Improvement Program (NSQIP) database was retrospectively reviewed to identify all patients who received lumbar decompression procedures in 2011. Risk-adjusted multivariate logistic regression analysis was performed to estimate independent predictors of UR.

Results

The overall rate of UR among patients undergoing lumbar decompression was 4.4%. After multivariate logistic regression analysis, anemia (odds ratio [OR] 1.48), dependent functional status (OR 3.03), total operative duration (OR 1.003), and American Society of Anesthesiologists Physical Status Class 4 (OR 3.61) remained as independent predictors of UR. Postoperative complications that were significantly associated with UR included overall complications (OR 5.18), pulmonary embolism (OR 3.72), and unplanned reoperation (OR 56.91).

Conclusions

There were several risk factors for UR after lumbar spine decompression surgery. Identification of high-risk patients and appropriate allocation of resources to reduce postoperative incidence may reduce the readmission rate.
Establishing Sprinkling Requirements on Trailers Transporting Market Weight Pigs in Warm and Hot Weather
Authors: Kephart Rebecca , Johnson Anna , Sapkota Avi , Stalder Kenneth , McGlone John
Published: April 2014

This study was conducted July of 2012 in Iowa, in WARM (<26.7 °C) and HOT (≥26.7 °C) weather. Four sprinkling methods were compared, with one treatment being randomly assigned to each load: control- no sprinkling (not applied in HOT weather), pigs only, bedding only, or pigs and bedding. Experiment 1 used 51 loads in WARM- and 86 loads in HOT weather to determine sprinkling effects on pig measures (surface temperature, vocalizations, slips and falls, and stress signs). Experiment 2 used 82 loads in WARM- and 54 loads in HOT weather to determine the sprinkling effects on transport losses (non-ambulatory, dead, and total transport losses). Experiment 1 found that, in WARM weather, there were no differences between sprinkling treatments for surface temperature, vocalizations, or slips and falls (p ≥ 0.18). However, stress signs were 2% greater when sprinkling pigs- or bedding only- compared to control (p = 0.03). Experiment 2 found that, in WARM and HOT weather, sprinkling did not affect non-ambulatory, dead, or total transport losses (p ≥ 0.18). Although the current study did not find any observed sprinkling effects for pig measures or transport losses it is extremely important to note that the inference space of this study is relatively small, so further studies should be conducted to see if these results are applicable to other geographical regions and seasons.
Perceived Stress Scores among Saudi Students Entering Universities: A Prospective Study during the First Year of University Life
Authors: Al-Daghri Nasser , Al-Othman Abdulaziz , Albanyan Abdulmajeed , Al-Attas Omar , Alokail Majed , Sabico Shaun , Chrousos George
Published: April 2014

In this prospective study we wanted to determine whether perceived stress over time among students in the Preparatory Year of King Saud University (KSU) predisposes them to cardiometabolic abnormalities. A total of 110 apparently healthy Saudi students (35 men and 75 women enrolled during the 2010−2011 academic year) were included. Perceived stress was determined at baseline and 1 year later. Anthropometrics were obtained and morning fasting serum glucose, lipid profile and cortisol were measured at both times. Perceived stress was noted among 48.2% of subjects at baseline and was not significantly different after follow-up, with 45.4% scoring high. In men, the prevalence of perceived stress was 48.6% at baseline (13 out of 35) and 37.1% at follow-up (13 out of 35), while in women it was 48% at baseline and 49.3% at follow-up. Interestingly, significant improvements in the blood pressure and lipid profiles, with the exception of HDL-cholesterol, were observed in both men and women, while fasting glucose also improved in women. Serum cortisol was inversely associated to fasting glucose, and total- and LDL-cholesterol (p-values 0.007, 0.04 and 0.04, respectively). These data are opposite to findings in students entering Western universities, in whom increasing stress and a deteriorating cardiometabolic profile have been repeatedly noted. Perceived stress and morning cortisol levels among students of the Preparatory Year in KSU remained constant for both genders over time, yet an improved cardiometabolic profile was observed, suggesting good adaptation among our pre-college students in their first year of university life.
RNA Sequencing Analysis Reveals Transcriptomic Variations in Tobacco (Nicotiana tabacum) Leaves Affected by Climate, Soil, and Tillage Factors
Authors: Lei Bo , Lu Kun , Ding Fuzhang , Zhang Kai , Chen Yi , Zhao Huina , Zhang Lin , Ren Zhu , Qu Cunmin , Guo Wenjing , Wang Jing , Pan Wenjie
Published: April 2014

The growth and development of plants are sensitive to their surroundings. Although numerous studies have analyzed plant transcriptomic variation, few have quantified the effect of combinations of factors or identified factor-specific effects. In this study, we performed RNA sequencing (RNA-seq) analysis on tobacco leaves derived from 10 treatment combinations of three groups of ecological factors, i.e., climate factors (CFs), soil factors (SFs), and tillage factors (TFs). We detected 4980, 2916, and 1605 differentially expressed genes (DEGs) that were affected by CFs, SFs, and TFs, which included 2703, 768, and 507 specific and 703 common DEGs (simultaneously regulated by CFs, SFs, and TFs), respectively. GO and KEGG enrichment analyses showed that genes involved in abiotic stress responses and secondary metabolic pathways were overrepresented in the common and CF-specific DEGs. In addition, we noted enrichment in CF-specific DEGs related to the circadian rhythm, SF-specific DEGs involved in mineral nutrient absorption and transport, and SF- and TF-specific DEGs associated with photosynthesis. Based on these results, we propose a model that explains how plants adapt to various ecological factors at the transcriptomic level. Additionally, the identified DEGs lay the foundation for future investigations of stress resistance, circadian rhythm and photosynthesis in tobacco.
Synthesis of Cellulose-2,3-bis(3,5-dimethylphenylcarbamate) in an Ionic Liquid and Its Chiral Separation Efficiency as Stationary Phase
Authors: Liu Runqiang , Zhang Yijun , Bai Lianyang , Huang Mingxian , Chen Jun , Zhang Yuping
Published: April 2014

A chiral selector of cellulose-2,3-bis(3,5-dimethylphenylcarbamate) (CBDMPC) was synthesized by reacting 3,5-dimethylphenyl isocyanate with microcrystalline cellulose dissolved in an ionic liquid of 1-allyl-3-methyl-imidazolium chloride (AMIMCl). The obtained chiral selector was effectively characterized by infrared spectroscopy, elemental analysis and 1H NMR. The selector was reacted with 3-aminopropylsilanized silica gel and the CBDMPC bonded chiral stationary phase (CSP) was obtained. Chromatographic evaluation of the prepared CSPs was conducted by high performance liquid chromatographic (HPLC) and baseline separation of three typical fungicides including hexaconazole, metalaxyl and myclobutanil was achieved using n-hexane/isopropanol as the mobile phase with a flow rate 1.0 mL/min. Experimental results also showed that AMIMCl could be recycled easily and reused in the preparation of CSPs as an effective reaction media.
Obesity and Its Metabolic Complications: The Role of Adipokines and the Relationship between Obesity, Inflammation, Insulin Resistance, Dyslipidemia and Nonalcoholic Fatty Liver Disease
Authors: Jung Un , Choi Myung-Sook
Published: April 2014

Accumulating evidence indicates that obesity is closely associated with an increased risk of metabolic diseases such as insulin resistance, type 2 diabetes, dyslipidemia and nonalcoholic fatty liver disease. Obesity results from an imbalance between food intake and energy expenditure, which leads to an excessive accumulation of adipose tissue. Adipose tissue is now recognized not only as a main site of storage of excess energy derived from food intake but also as an endocrine organ. The expansion of adipose tissue produces a number of bioactive substances, known as adipocytokines or adipokines, which trigger chronic low-grade inflammation and interact with a range of processes in many different organs. Although the precise mechanisms are still unclear, dysregulated production or secretion of these adipokines caused by excess adipose tissue and adipose tissue dysfunction can contribute to the development of obesity-related metabolic diseases. In this review, we focus on the role of several adipokines associated with obesity and the potential impact on obesity-related metabolic diseases. Multiple lines evidence provides valuable insights into the roles of adipokines in the development of obesity and its metabolic complications. Further research is still required to fully understand the mechanisms underlying the metabolic actions of a few newly identified adipokines.
Substance P Activates the Wnt Signal Transduction Pathway and Enhances the Differentiation of Mouse Preosteoblastic MC3T3-E1 Cells
Authors: Mei Gang , Zou Zhenlv , Fu Su , Xia Liheng , Zhou Jian , Zhang Yongtao , Tuo Yonghua , Wang Zhao , Jin Dan
Published: April 2014

Recent experiments have explored the impact of Wnt/β-catenin signaling and Substance P (SP) on the regulation of osteogenesis. However, the molecular regulatory mechanisms of SP on the formation of osteoblasts is still unknown. In this study, we investigated the impact of SP on the differentiation of MC3T3-E1 cells. The osteogenic effect of SP was observed at different SP concentrations (ranging from 10−10 to 10−8 M). To unravel the underlying mechanism, the MC3T3-E1 cells were treated with SP after the pretreatment by neurokinin-1 (NK1) antagonists and Dickkopf-1 (DKK1) and gene expression levels of Wnt/β-catenin signaling pathway components, as well as osteoblast differentiation markers (collagen type I, alkaline phosphatase, osteocalcin, and Runx2), were measured using quantitative polymerase chain reaction (PCR). Furthermore, protein levels of Wnt/β-catenin signaling pathway were detected using Western blotting and the effects of SP, NK1 antagonist, and DKK1 on β-catenin activation were investigated by immunofluorescence staining. Our data indicated that SP (10−9 to 10−8 M) significantly up-regulated the expressions of osteoblastic genes. SP (10−8 M) also elevated the mRNA level of c-myc, cyclin D1, and lymphocyte enhancer factor-1 (Lef1), as well as c-myc and β-catenin protein levels, but decreased the expression of Tcf7 mRNA. Moreover, SP (10−8 M) promoted the transfer of β-catenin into nucleus. The effects of SP treatment were inhibited by the NK1 antagonist and DKK1. These findings suggest that SP may enhance differentiation of MC3T3-E1 cells via regulation of the Wnt/β-catenin signaling pathway.
The Epicardium in the Embryonic and Adult Zebrafish
Authors: Peralta Marina , González-Rosa Juan , Marques Inês , Mercader Nadia
Published: April 2014

The epicardium is the mesothelial outer layer of the vertebrate heart. It plays an important role during cardiac development by, among other functions, nourishing the underlying myocardium, contributing to cardiac fibroblasts and giving rise to the coronary vasculature. The epicardium also exerts key functions during injury responses in the adult and contributes to cardiac repair. In this article, we review current knowledge on the cellular and molecular mechanisms underlying epicardium formation in the zebrafish, a teleost fish, which is rapidly gaining status as an animal model in cardiovascular research, and compare it with the mechanisms described in other vertebrate models. We moreover describe the expression patterns of a subset of available zebrafish Wilms’ tumor 1 transgenic reporter lines and discuss their specificity, applicability and limitations in the study of epicardium formation.
A Procedure to Measure the in-Situ Hygrothermal Behavior of Earth Walls
Authors: Chabriac Pierre-Antoine , Fabbri Antonin , Morel Jean-Claude , Laurent Jean-Paul , Blanc-Gonnet Joachim
Published: April 2014

Rammed earth is a sustainable material with low embodied energy. However, its development as a building material requires a better evaluation of its moisture-thermal buffering abilities and its mechanical behavior. Both of these properties are known to strongly depend on the amount of water contained in wall pores and its evolution. Thus the aim of this paper is to present a procedure to measure this key parameter in rammed earth or cob walls by using two types of probes operating on the Time Domain Reflectometry (TDR) principle. A calibration procedure for the probes requiring solely four parameters is described. This calibration procedure is then used to monitor the hygrothermal behavior of a rammed earth wall (1.5 m × 1 m × 0.5 m), instrumented by six probes during its manufacture, and submitted to insulated, natural convection and forced convection conditions. These measurements underline the robustness of the calibration procedure over a large range of water content, even if the wall is submitted to quite important temperature variations. They also emphasize the importance of gravity on water content heterogeneity when the saturation is high, as well as the role of liquid-to-vapor phase change on the thermal behavior.
Preparation of Nanocomposite Plasmonic Films Made from Cellulose Nanocrystals or Mesoporous Silica Decorated with Unidirectionally Aligned Gold Nanorods
Authors: Campbell Michael , Liu Qingkun , Sanders Aric , Evans Julian , Smalyukh Ivan
Published: April 2014

Using liquid crystalline self-assembly of cellulose nanocrystals, we achieve long-range alignment of anisotropic metal nanoparticles in colloidal nanocrystal dispersions that are then used to deposit thin structured films with ordering features highly dependent on the deposition method. These hybrid films are comprised of gold nanorods unidirectionally aligned in a matrix that can be made of ordered cellulose nanocrystals or silica nanostructures obtained by using cellulose-based nanostructures as a replica. The ensuing long-range alignment of gold nanorods in both cellulose-based and nanoporous silica films results in a polarization-sensitive surface plasmon resonance. The demonstrated device-scale bulk nanoparticle alignment may enable engineering of new material properties arising from combining the orientational ordering of host nanostructures and properties of the anisotropic plasmonic metal nanoparticles. Our approach may also allow for scalable fabrication of plasmonic polarizers and nanoporous silica structures with orientationally ordered anisotropic plasmonic nanoinclusions.
Stable Isotope Tracer Analysis in Isolated Mitochondria from Mammalian Systems
Authors: Gravel Simon-Pierre , Andrzejewski Sylvia , Avizonis Daina , St-Pierre Julie
Published: April 2014

Mitochondria are a focal point in metabolism, given that they play fundamental roles in catabolic, as well as anabolic reactions. Alterations in mitochondrial functions are often studied in whole cells, and metabolomics experiments using 13C-labeled substrates, coupled with mass isotopomer distribution analyses, represent a powerful approach to study global changes in cellular metabolic activities. However, little is known regarding the assessment of metabolic activities in isolated mitochondria using this technology. Studies on isolated mitochondria permit the evaluation of whether changes in cellular metabolic activities are due to modifications in the intrinsic properties of the mitochondria. Here, we present a streamlined approach to accurately determine 13C, as well as 12C enrichments in isolated mitochondria from mammalian tissues or cultured cells by GC/MS. We demonstrate the relevance of this experimental approach by assessing the effects of drugs perturbing mitochondrial functions on the mass isotopomer enrichment of metabolic intermediates. Furthermore, we investigate 13C and 12C enrichments in mitochondria isolated from cancer cells given the emerging role of metabolic alterations in supporting tumor growth. This original method will provide a very sensitive tool to perform metabolomics studies on isolated mitochondria.
Different Accumulation Profiles of Multiple Components Between Pericarp and Seed of Alpinia oxyphylla Capsular Fruit as Determined by UFLC-MS/MS
Authors: Chen Feng , Li Hai-Long , Tan Yin-Feng , Guan Wei-Wei , Zhang Jun-Qing , Li Yong-Hui , Zhao Yuan-Sheng , Qin Zhen-Miao
Published: April 2014

Plant secondary metabolites are known to not only play a key role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals. Alpinia oxyphylla capsular fruits, made up of seeds and pericarps, are commonly used in traditional East Asian medicines. In clinical utilization of these capsular fruits, inconsistent processing approaches (i.e., hulling pericarps or not) are employed, with the potential of leading to differential pharmacological effects. Therefore, an important question arises whether the content levels of pharmacologically active chemicals between the seeds and pericarps of A. oxyphylla are comparable. Nine secondary metabolites present in A. oxyphylla capsular fruits, including flavonoids (e.g., tectochrysin, izalpinin, chrysin, apigenin-4',7-dimethylether and kaempferide), diarylheptanoids (e.g., yakuchinone A and B and oxyphyllacinol) and sesquiterpenes (e.g., nootkatone), were regarded as representative constituents with putative pharmacological activities. This work aimed to investigate the abundance of the nine constituents in the seeds and pericarps of A. oxyphylla. Thirteen batches of A. oxyphylla capsular fruits were gathered from different production regions. Accordingly, an ultra-fast high performance liquid chromatography/quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed and validated. We found that: (1) the nine secondary metabolites were differentially concentrated in seeds and fruit capsules; (2) nootkatone is predominantly distributed in the seeds; in contrast, the flavonoids and diarylheptanoids are mainly deposited in the capsules; and (3) the content levels of the nine secondary metabolites occurring in the capsules varied greatly among different production regions, although the nootkatone levels in the seeds were comparable among production regions. These results are helpful to evaluating and elucidating pharmacological activities of A. oxyphylla capsular fruits. Additionally, it may be of interest to elucidate the mechanisms involved in the distinct accumulation profiles of these secondary metabolites between seeds and pericarps.
Dyadic Adjustment and Spiritual Activities in Parents of Children with Cystic Fibrosis
Authors: Grossoehme Daniel , Szczesniak Rhonda , Dodd Caitlin , Opipari-Arrigan Lisa
Published: April 2014

Children’s diseases can negatively impact marital adjustment and contribute to poorer child health outcomes. To cope with increased marital stress and childhood diseases severity, many people turn to spirituality. While most studies show a positive relationship between spirituality and marital adjustment, spirituality has typically been measured only in terms of individual behaviors. Using the Dyadic Adjustment Scale (DAS) and Daily Phone Diary data from a sample of 126 parents of children with cystic fibrosis as a context for increased marital stress, spiritual behavior of mother-father dyads and of whole families were used as predictors of marital adjustment. Frequency and duration of individual, dyadic and familial spiritual activities correlated positively with dyadic adjustment. Significant differences in spiritual activities existed between couples with marital adjustment scores above and below the cutoff for distress. The only significant factors in regressions of spiritual activities on marital adjustment scores were number of pulmonary exacerbations and parent age. Higher odds of maintaining a marital adjustment score greater than 100 were significantly associated with spending approximately twelve minutes per day in individual, but not conjugal or familial, spiritual activities. The Daily Phone Diary is a feasible tool to study conjugal and familial activities and their relationships with beliefs and attitudes, including spirituality.
Estimation of Diurnal Cycle of Land Surface Temperature at High Temporal and Spatial Resolution from Clear-Sky MODIS Data
Authors: Duan Si-Bo , Li Zhao-Liang , Tang Bo-Hui , Wu Hua , Tang Ronglin , Bi Yuyun , Zhou Guoqing
Published: April 2014

The diurnal cycle of land surface temperature (LST) is an important element of the climate system. Geostationary satellites can provide the diurnal cycle of LST with low spatial resolution and incomplete global coverage, which limits its applications in some studies. In this study, we propose a method to estimate the diurnal cycle of LST at high temporal and spatial resolution from clear-sky MODIS data. This method was evaluated using the MSG-SEVIRI-derived LSTs. The results indicate that this method fits the diurnal cycle of LST well, with root mean square error (RMSE) values less than 1 K for most pixels. Because MODIS provides at most four observations per day at a given location, this method was further evaluated using only four MSG-SEVIRI-derived LSTs corresponding to the MODIS overpass times (10:30, 13:30, 22:30, and 01:30 local solar time). The results show that the RMSE values using only four MSG-SEVIRI-derived LSTs are approximately two times larger than those using all LSTs. The spatial distribution of the modeled LSTs at the MODIS pixel scale is presented from 07:00 to 05:00 local solar time of the next day with an increment of 2 hours. The diurnal cycle of the modeled LSTs describes the temporal evolution of the LSTs at the MODIS pixel scale.
Extraction of Urban Power Lines from Vehicle-Borne LiDAR Data
Authors: Cheng Liang , Tong Lihua , Wang Yu , Li Manchun
Published: April 2014

Airborne LiDAR has been traditionally used for power line cruising. Nevertheless, data acquisition with airborne LiDAR is constrained by the complex environments in urban areas as well as the multiple parallel line structures on the same power line tower, which means it is not directly applicable to the extraction of urban power lines. Vehicle-borne LiDAR system has its advantages upon airborne LiDAR and this paper tries to utilize vehicle-borne LiDAR data for the extraction of urban power lines. First, power line points are extracted using a voxel-based hierarchical method in which geometric features of each voxel are calculated. Then, a bottom-up method for filtering the power lines belonging to each power line is proposed. The initial clustering and clustering recovery procedures are conducted iteratively to identify each power line. The final experiment demonstrates the high precision of this technique.
Modeling and Manufacturing of a Micromachined Magnetic Sensor Using the CMOS Process without Any Post-Process
Authors: Tseng Jian-Zhi , Wu Chyan-Chyi , Dai Ching-Liang
Published: April 2014

The modeling and fabrication of a magnetic microsensor based on a magneto-transistor were presented. The magnetic sensor is fabricated by the commercial 0.18 mm complementary metal oxide semiconductor (CMOS) process without any post-process. The finite element method (FEM) software Sentaurus TCAD is utilized to analyze the electrical properties and carriers motion path of the magneto-transistor. A readout circuit is used to amplify the voltage difference of the bases into the output voltage. Experiments show that the sensitivity of the magnetic sensor is 354 mV/T at the supply current of 4 mA.
Object Detection Techniques Applied on Mobile Robot Semantic Navigation
Authors: Astua Carlos , Barber Ramon , Crespo Jonathan , Jardon Alberto
Published: April 2014

The future of robotics predicts that robots will integrate themselves more every day with human beings and their environments. To achieve this integration, robots need to acquire information about the environment and its objects. There is a big need for algorithms to provide robots with these sort of skills, from the location where objects are needed to accomplish a task up to where these objects are considered as information about the environment. This paper presents a way to provide mobile robots with the ability-skill to detect objets for semantic navigation. This paper aims to use current trends in robotics and at the same time, that can be exported to other platforms. Two methods to detect objects are proposed, contour detection and a descriptor based technique, and both of them are combined to overcome their respective limitations. Finally, the code is tested on a real robot, to prove its accuracy and efficiency.
Spatial Estimation of Sub-Hour Global Horizontal Irradiance Based on Official Observations and Remote Sensors
Authors: Gutierrez-Corea Federico-Vladimir , Manso-Callejo Miguel-Angel , Moreno-Regidor María-Pilar , Velasco-Gómez Jesús
Published: April 2014

This study was motivated by the need to improve densification of Global Horizontal Irradiance (GHI) observations, increasing the number of surface weather stations that observe it, using sensors with a sub-hour periodicity and examining the methods of spatial GHI estimation (by interpolation) with that periodicity in other locations. The aim of the present research project is to analyze the goodness of 15-minute GHI spatial estimations for five methods in the territory of Spain (three geo-statistical interpolation methods, one deterministic method and the HelioSat2 method, which is based on satellite images). The research concludes that, when the work area has adequate station density, the best method for estimating GHI every 15 min is Regression Kriging interpolation using GHI estimated from satellite images as one of the input variables. On the contrary, when station density is low, the best method is estimating GHI directly from satellite images. A comparison between the GHI observed by volunteer stations and the estimation model applied concludes that 67% of the volunteer stations analyzed present values within the margin of error (average of ±2 standard deviations).
Toward a Remote Sensing Solution for Regional Sustainability Assessment and Monitoring
Authors: Lein James
Published: April 2014

Regional sustainability encourages a re-examination of development programs in the context of environmental, social and economic policies and practices. However, sustainability remains a broadly defined concept that has been applied to mean everything from environmental protection, social cohesion, economic growth, neighborhood design, alternative energy, and green building design. To guide sustainability initiatives and assess progress toward more sustainable development patterns, a need exists to place this concept into a functional decision-centric context where change can be evaluated and the exploitation of resources better understood. Accepting the premise that sustainable development defines a set of conditions and trends in a given system that can continue indefinitely without contributing to environmental degradation, answers to four critical questions that direct sustainability over the long-term must be addressed: (1) What is the present state of the environmental system, (2) Is that pattern sustainable, (3) Are there indications that the environmental system is degrading, and (4) Can that information be incorporated into policy decisions to guide the future? Answers to these questions hinge on the development of tractable indices that can be employed to support the long-term monitoring required to assess sustainability goals and a means to measure those indices. In this paper, a solution based on the application of remote sensing technology is introduced focused on the development of land use intensity indices derived from earth-observation satellite data. Placed into a monitoring design, this approach is evaluated in a change detection role at the watershed scale.