bluuebunny/crossref_metadata_2025 huggingface dataset:
Limits of Detection Analysis of Advanced Technologies for Bacterial Detection in Food Samples: Review & Future Perspective
Authors: Zhao Xinyi , Bhat Abhijnan , O’Connor Christine , Curtin James , Singh Baljit , Tian Furong
Published: January 2024

Foodborne illnesses can be infectious and dangerous and most of them are caused by bacteria. Some types of common food-related bacteria exist widely in nature and pose a serious threat to both humans and animals, and can cause poisoning, diseases, disabilities and even death. Rapid, reliable and cost-effective methods for bacteria detection are of paramount importance in food-safety and environmental monitoring. Polymerase chain reaction (PCR), lateral flow immunochromatographic assay (LFIA) and electrochemical methods have been widely used in food-safety and environmental monitoring. In this paper, the recent developments (2013-2023) covering PCR, LFIA and electrochemical methods for various bacteria detection (Salmonella, Listeria, Campylobacter, Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli)) considering different foods types, analytical performances and the reported limit of detection (LOD) are discussed. It is found that the bacteria type and food sample type contribute significantly to the analytical per-formance and LOD. Detection by LFIA has higher average LOD (24 CFU/ml) than detection by electrochemical methods (12 CFU/ml) and PCR (6 CFU/ml). Salmonella and E. coli in the Pseudomonadota domain usually have low LODs. LODs are usually lower for detection in fish and eggs. LFIA with gold and iron nanoparticles are prominent in the majority of articles of 26 CFU/ml and 12 CFU/ml respectively. Electrochemical methods reveal that the average LOD is highest for cyclic voltammetry (CV) of 18 CFU/ml), followed by electrochemical impedance spectroscopy (EIS) of 12 CFU/ml and differential pulse voltammetry (DPV) of 8 CFU/ml. Finally, the review discusses the challenges and future perspectives (including the role of nanomaterials/advanced materials) to improve the analytical performance for bacterial detection.
FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols
Authors: Bustin Stephen Andrew , Kirvell Sara , Nolan Tania , Shipley Gregory L.
Published: January 2024

Versatility, sensitivity and accuracy have made the real-time polymerase chain reaction (qPCR) a crucial tool for research as well as diagnostic applications. However, for point-of-care (PoC) use traditional qPCR faces two main challenges: long run times mean results are not available for half an hour or more and the requisite high-temperature denaturation requires more robust and pow-er-demanding instrumentation. This study addresses both issues and revised primer and probe designs, modified buffers and low ∆T protocols which, together, speed up qPCR on conventional qPCR instruments and will allow the development of robust, point-of-care devices. Our approach, called "FlashPCR", also allows efficient reverse transcription as part of a one-step RT-qPCR pro-tocol, making it universally applicable for both rapid research and diagnostic applications.
Stigma pada tokoh utama dalam Novel 813 karya Maurice Leblanc
Authors: Rahma Adelia  , Ninuk Lustyantie  , Wahyu Tri Widyastuti
Published: January 2024

Tujuan penelitian ini adalah untuk mendeskripsikan fase-fase pembentuk stigma pada tokoh utama yang terdapat dalam Novel 813 karya Maurice Leblanc. Pendekatan yang digunakan adalah pendekatan kualitatif struktural karya sastra dengan metode analisis isi. Data berupa kata, frasa, kalimat atau dialog tentang fase-fase pembentuk stigma. Sedangkan sumber data adalah Novel 813 karya Maurice Leblanc. Teori yang digunakan dalam penelitian ini mengacu pada teori stigma menurut Jean-Yves Giordana berupa tiga fase-fase pembentukan stigma yaitu (1) fase pelabelan (Étiquetage) (2) prasangka (Préjudice), dan (3) diskriminasi (Discrimination). Analisis data menggunakan studi dokumen dan mengacu pada teknik analisis data untuk sumber data yaitu Novel 813 karya Maurice Leblanc dengan menganalisis pada setiap unsur intrinsik pada novel mulai dari alur, tokoh dan latar, kemudian mengacu pada teknik analisis data yang digunakan oleh Miles dan Huberman yaitu, reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa ada dua puluh dua (22) kutipan yang menunjukkan sebuah fase-fase pembentuk stigma yang dirasakan tokoh utama dalam novel 813 karya Maurice Leblanc. Di antaranya yang pertama fase prasangka berjumlah sebelas (11) kutipan, fase pelabelan berjumlah tujuh (7) kutipan dan fase diskriminasi berjumlah empat (4) kutipan. Berdasarkan penelitian yang telah selesai dilakukan dapat disimpulkan fase prasangka menjadi fase yang paling banyak dirasakan oleh tokoh utama dalam novel 813 karya Maurice Leblanc, kemudian disusul oleh fase kedua adalah fase pelabelan dan yang terakhir adalah fase diskriminasi.
Tingkat kesesuaian latihan bahasa Prancis dalam situs pembelajaran apprendre.tv5monde.com dengan capaian pembelajaran bahasa Prancis tingkat A2 di SMA
Authors: Nur Dwi Cahyani Ismi , Sri Harini Ekowati  , Ratna
Published: January 2024

Penelitian ini bertujuan untuk mengetahui tingkat kesesuaian latihan Bahasa Prancis dalam situs pembelajaran apprendre.tv5monde.com dengan capaian belajar Bahasa Prancis tingkat A2 di SMA. Dalam upaya memaksimalkan tujuan kurikulum merdeka belajar, situs pembelajaran apprendre.tv5monde.com merupakan salah satu sumber belajar alternatif yang menyediakan latihan Bahasa Prancis untuk mendukung siswa dalam belajar Bahasa Prancis secara mandiri dan bebas. Namun, pemanfaatan situs belajar sebagai sumber belajar seyogyanya harus sesuai dengan capaian pembelajaran agar tujuan pembelajaran dapat tercapai dengan baik. Penelitian ini adalah penelitian kualitatif yang menggunakan teknik analisis isi (content analysis) dan menggunakan studi dokumentasi sebagai teknik pengumpulan data. Sumber data yang digunakan adalah latihan bahasa Prancis dalam situs apprendre.tv5monde.com. Data yang telah dikumpulkan dianalisis dengan menggunakan metode checklist. Hasil penelitian ini menunjukkan terdapat sebanyak 34 latihan yang sesuai dengan capaian pembelajaran Bahasa Prancis SMA tingkat A2 berdasarkan CECR dari total 41 latihan. Hasil perhitungan tersebut kemudian dihitung dengan menggunakan perhitungan presentase dengan hasil persentase sebesar 82.92%. Maka sesuai kriteria persentase kesesuaian oleh Arikunto (2006), latihan Bahasa Prancis dalam situs pembelajaran apprendre.tv5monde.com sesuai dengan capaian pembelajaran SMA tingkat A2.
Membangun karakter nasionalisme pada siswa melalui novel Lingkar Tanah Lingkar Air karangan Ahmad Tohari
Authors: Dewi Quraisyin Switia , Ummu Sovia  , Sri Harini Ekowati  , Zainal Rafli
Published: January 2024

Penelitian ini bertujuan untuk mendeskripsikan nilai nasionalisme yang terdapat pada novel Lingkar Tanah, Lingkar Air  karangan Ahmad Tohari yang mampu untuk membangun karakter siswa. Penelitian ini menganalisis 5 bentuk nilai nasionalisme yaitu proses pembentukan dan  pertumbuhan bangsa, sentimen atau kesadaran memiliki bangsa, bahasa dan simbolisme bangsa, gerakan sosial dan politik demi bangsa, serta doktrin dan  ideologi bangsa dengan objek penelitian novel dan siswa. Penelitian ini merupakan jenis penelitian deskriptif-kualitatif dan mengambil data dari objek yang sudah ada yaitu berupa kutipan kata, frasa, atau kalimat yang terdapat dalam novel tersebut, data tersebut kemudian dideskripsikan melalui penjelasan interpretatif berdasarkan teori yang digunakan dalam penelitian. Analisis data dalam penelitian ini merupakan teknik analisis data dari Miles dan Huberman. Dari penelitian yang dilaksanakan, dapat diketahui pertama, Jumlah keseluruhan bentuk nilai nasionalisme yang terdapat pada novel Lingkar Tanah Lingkar Air yaitu 81 data. Kedua, Narasi yang dilakukan oleh tokoh dalam novel ini secara eksplisit menjadi bukti bahwa terdapat nilai nasionalisme yang dirasa cocok untuk penanaman nilai nasionalisme. Ketiga, pembelajaran sastra yang lebih banyak teori membuat siswa menjadi bosan dan merasa tidak minat. Perlu praktik secara langsung dan siswa harus terjun langsung dalam memahami sebuah karya sastra. Keempat, Siswa sangat menyukai bermain peran pada pembelajaran novel karena seru dan tidak membosankan. Siswa menganggap novel menjadi lebih menarik dan kata-katanya lebih mudah untuk dimengerti, selain itu, novel dianggap memberikan ilmu dan pengetahuan yang belum siswa ketahui sebelumnya.
Maggie O'Farrell's «Hamnet»: an Imaginative Critical-Biographical Interpretation of William Shakespeare's «Hamlet»
Authors: García Santos Patricia
Published: January 2024

The aim of this essay is to contribute to the understanding of William Shakespeare’s tragedy Hamlet (1603) by means of a critical reconsideration of biographical episodes such as the functional gap the playwright left in his family. To this end, two texts will be examined, Maggie O’Farrell’s Hamnet (2020) and Shakespeare’s Hamlet itself, reading the former as an imaginative critical-biographical interpretation of the latter. The focus will be on Shakespeare’s domestic life, particularly on the figure of his son Hamnet and the relationship he had with his father, as depicted in O’Farrell’s work. Using the dramatic text as primary textual source, a thorough comparative interpretation of both literary pieces will be carried out. The analysis of key passages of the novel will lend support to the hypothesis that Hamnet can be considered as a critical-biographical interpretation of Shakespeare’s Hamlet.
«Healing is Like Falling in Love, but Deeper»: Romance and Intertextuality in Junauda Petrus’s «The Stars and the Blackness Between Them»
Authors: Pierini Francesca
Published: January 2024

This article examines Junauda Petrus’s The Stars and the Blackness Between Them (2020) – a literary debut telling the story of two black girls from different backgrounds falling in love with each other – in light of John Green’s The Fault in Our Stars (2012). After briefly introducing the subgenre of YA queer romance fiction, the article adopts a popular romance studies perspective to observe how the presence of the protagonist’s terminal illness affects the narrative construction of adolescent love. In its first part, the article specifically aims at shedding light on a discursive aspect – shared by Petrus’s and Green’s narratives – centring on a notion of existential infinitude within finitude brought about by recognition. The second part of the article discusses intertextuality as a substantial formal aspect that reveals Green’s and Petrus’s different approaches to the genre they engage with – articulated in terms of the Symbolic and the Semiotic respectively – and the way they narrate and resolve the destinies of their fictional characters.
The Influence of Islamic Banks and Sovereign Retail Sukuk on Economic Growth in Indonesia
Authors: Bella Firsty Izzata , Inas Inas
Published: January 2024

This study aims to examine the short-term and long-term effects of Islamic banking financing and the development of sovereign Retail Sukuk (SR) on Indonesia's economic growth during the period 2009: Q1 to 2019: Q3. Islamic banking and Sukuk have the same essential role, namely in terms of financing or raising funds. Outstanding SR is used as an indicator of SR’s development in seeing its impact on Indonesia's economic growth. Through Dickey Fuller-Generalized Least Square (DF-GLS) analysis, Lag-Length Test, Auto-Regressive Distributed Lag (ARDL), Cointegration Bound Testing, this study examines the effect of Islamic banking financing and the development of SR on economic growth. Total Sharia Bank financing and outstanding SR do not have long-term cointegration with Indonesia's economic growth. Meanwhile, in the short term, Indonesia's GDP is influenced positively by total Islamic Bank financing (TFIN) at lag 3 and negatively by the outstanding SR at lag 3 and 4. Researchers only examined the Islamic banking sector, specifically highlighting financing in Islamic banking and SR’s development through nominal outstanding on a quarterly scale. The limitations of the variables studied are becoming the limitations of this study. The government as a policymaker have to provide a support through cooperation between institutions and Medium and Small Enterprises (MSMEs) with Islamic banks in collecting and channeling financing, education, and outreach to the public. Consequently, the deepest layers need to be improved to make SR an individual investment instrument that can support Indonesia's economic growth. The research that examined SR with quantitative methods is still limited. Therefore, this study is expected to contribute to increasing liabilities in Islamic Finance, particularly in Retail Sukuk.
Apakah Promosi Islami dan Hedonic Shopping Motivation Mempengaruhi Perilaku Impulsive Buying Generasi Z?
Authors: Azid Raka Malik , Machfudz Masyhuri
Published: January 2024

The emergence of Muslim fashion in Banyuwangi can be attributed to the inclination of the Z generation to utilize e-commerce platforms for fashion shopping, hence promoting impulsive purchasing behavior.   The current study aims to examine how the perception of Islamic promotion and hedonic shopping motives influence impulse purchases, with happy emotions serving as mediators in the context of Muslim fashion products.   This study focuses on clients belonging to Generation Z in Banyuwangi Regency who have made purchases using e-commerce platforms.   This study employs a quantitative research design with an explanatory approach. It has a sample size of 100 participants selected using purposive sampling methods.    The survey results obtained through an online questionnaire with a Likert scale were subsequently analyzed using path analysis in SPSS.   The findings of this study indicate that employing Islamic advertising and hedonic impacts can enhance consumers' favorable emotions towards Muslim fashion products via e-commerce.   Furthermore, the indirect impact of favorable emotions serves as a mediator for the effect of of islamic promotion on impulsive purchasing. However, it is important to note that various good emotions do not mediate the relationship between hedonic shopping incentive and impulsive purchase.
Propagating the Concept of Rahmatan Lil ‘Aalamin through Suffistic Da’wah in East Kalimantan
Authors: Noorthaibah Noorthaibah , Maula Ishmatul
Published: January 2024

Dawah plays a vital role in developing and disseminating Islam as it opens opportunities for Islamic teachings to be accepted and adapted in new environments with people from different cultures. In this context, Sufi preaching is one of the successful manifestations in the propagation of Islam due to its flexible nature, which is its characteristic feature. Therefore, this research aims to examine and identify the role of preaching in developing Sufism in East Kalimantan, particularly in the current era of globalization. This study employs a descriptive qualitative research approach, with research locations spread across several areas in East Kalimantan, including Samarinda, Kutai Kartanegara, Balikpapan, Kutai Timur, Bontang, and Paser. The research results indicate that the current societal conditions closely related to technological advancements and information necessitate the presence of more relevant forms of preaching. In this context, da'is are obliged to use digital media to convey Sufi teachings. They are expected to be proficient in utilizing technology to continue delivering their preaching in line with the progress of the times.
Spiritual Communication of Selakaran Among Sasak Muslim Community During The Covid-19 Pandemic
Authors: Syaoki Muhammad
Published: January 2024

The COVID-19 pandemic has introduced unparalleled challenges to individuals worldwide, resulting in substantial alterations in lifestyles. These alterations encompass the adoption of health protocols, such as social distancing and frequent handwashing. Nevertheless, it is noteworthy that certain Sasak Muslim communities have opted for a distinctive approach in confronting the pandemic. Their chosen strategy revolves around the performance of rituals. These rituals, executed collectively, are perceived by the Sasak Muslim community as a means to counteract the global COVID-19 pandemic. To comprehensively explore the significance of these rituals within the Sasak Muslim community's response to the COVID-19 pandemic, this qualitative research employs an ethnographic approach. The overarching goal is to gain insight into how these rituals are construed and employed by the community. The study's findings unveil that the Sasak Muslim community perceives the pandemic as a typical ailment, one that can be alleviated through a deepened connection with Allah. One of the primary methods through which this closeness is sought is an increased engagement in dhikr, which involves the remembrance of Allah, and prayers performed within the framework of specific rituals such as Selakaran.
The Shift of University Students’ Communication Ethics in Post-COVID-19 Pandemic Learning
Authors: Martini Endang , Yuliarti Monika Sri , Prakoso Septyanto Galan , Budiwati Christiyaningsih
Published: January 2024

The COVID-19 pandemic has left behind a wide range of effects. As a potential future learning paradigm, one of them is the hybrid learning model. In terms of appearance during lectures, whether directly or indirectly through the use of post-COVID-19 pandemic media, this study attempts to characterize the communication ethics of generation Z students at Sebelas Maret University Vocational School in Surakarta. The quantitative descriptive method is thought to be the most appropriate approach to use in addressing the study objectives. Secondary data is derived from internal data in the education sector, publications, books, and other documentation, while primary data is collected online using semi-open questions in addition to observation. It is possible to conclude that there is a lack of good student communication ethics when it comes to appearance during online learning because not all students have turned on their cameras, not all of them are dressed collared, and not all of them follow the dress code that has been established in accordance with previous agreements in a number of courses.
Preparation and Performance Evaluation of Quaternary Copolymer Sand Control Agent
Authors: Duan Ming , Wang Qihai , Chen Bin , Chen Huaxin , Li Xinliang , Fang Shenwen
Published: January 2024

Sand production is a common problem in petroleum extraction, and the development of water-soluble polymer sand control agents is currently a research hotspot. However, this research does not provide details of the chemical structure and lacks discussions on adsorption and sand control mechanisms. Herein, a new water-soluble polymer sand control agent (PAADD) is reported on; it is a copolymer of 2-acrylamido-2-methylpropane sulfonic acid (AMPS), N-(3-dimethylaminopropyl) methacrylamide (DPM), a functional monomer (DAM) containing ketone groups and self-crosslinking properties, and acrylamide (AM). First, the synthesis conditions of PAADD are optimized, and then its sand control mechanism is explored. Experimental results reveal that PAADD can adsorb on the sand surface through electrostatic attraction and hydrogen bonding and change the surface properties of sand particles, which promotes the aggregation of sand particles and permits good sand control ability. In flow experiments, after the injection of PAADD solution (500 mg/L) into a sandpacked model with a permeability of 500 md, the critical sand production rate of the sandpacked model increased more than 100 times and the permeability damage rate (PDR) was 30–40%.
Design and Validation of Flim-timer for Measuring Protein Turnover in Cells Using Fluorescence Lifetime
Authors: Dikovskaya Dina , Bento-Pereira Claudia , Shiga Kanade , Corno Andrea , Higgins Maureen , Toth Rachel , Saurin Adrian , Dinkova-Kostova Albena
Published: January 2024

Measuring protein turnover in individual cells and subcellular compartments has been greatly assisted by fluorescence timers (FT) whose changing spectra, expressed as the ratio of fluorescence intensities, reflect their molecular age. However, FT quantification requires highly fluorescent samples, prohibiting their use for proteins with low or non-uniform expression. Here we developed a method that utilises fluorescence lifetime, a property largely independent of intensity, of a specifically designed FT (named FLIM-timer). The fluorescence lifetime of FLIM-timer reflected protein turnover in regions of high and low brightness with equally high precision. We demonstrate the versatility of this approach with several versions of FLIM-timer in two distinct experimental systems: 1) as a readout for efficacy of pharmacological inducers of Nrf2, the master regulator of redox homeostasis, and 2) as a tool to monitor cyclin B1 degradation during mitotic exit. Overall, the FLIM-timer methodology expands the applicability of FT for visualisation and quantification of protein turnover.
Comprehensive Analysis of Cuproptosis-related Genes Involved in Prognosis and Tumor Microenvironment Infiltration of Colorectal Cancer
Authors: Chen Weiyi , Hu Ke , Liu Yu , Li Xiaocheng , Peng Jing , Chen Lijun , Duan Shaoyi , Yang Qizhang
Published: January 2024

Colorectal cancer (CRC) is a common malignancy with high incidence and mortality rates. Cuproptosis, a novel form of copper-induced programmed cell death, contributes to tumor progression. Whether cuproptosis-related genes (CRGs) play a role in CRC remains unclear. Here, we systematically assessed CRG mutations in CRC. The expression of eight of the 10 CRGs was significantly different between CRC and normal tissues. CDKN2A and DLAT were identified as independent risk factors for predicting overall survival (OS) in CRC using univariate and multivariate Cox analyses, and a risk score model was constructed based on CDKN2A and DLAT. Patients with CRC were categorized into high- and low-risk groups based on their median risk scores, with those in the low-risk group having longer OS than those in the high-risk group. Receiver operating characteristic curve analysis indicated that the risk score model had good predictive accuracy for OS. Analysis of the TISCH and TISIDB database showed that CDKN2A and DLAT were associated with the TME, and the molecular subtypes and immune subtypes differed between CDKN2A and DLAT. Spearman’s analysis showed that CDKN2A and DLAT expressions were significantly associated with immune cell infiltration in CRC. Our research revealed CRGs’ prognostic value, particularly CDKN2A and DLAT, in CRC and demonstrated the relationship between CDKN2A/DLAT and immune infiltration in CRC, thereby contributing to the outcome evaluation of patients with CRC and identifying novel targets for CRC immunotherapy.
Accumulation of lncRNAs in cytoplasm upon DIS3 depletion leads to production of cryptic peptides detected in Multiple Myeloma.
Authors: Foretek Dominika , Gabriel Marc , Hatin Isabelle , Jarroux Julien , Pinskaya Marina , Pepermans Elise , Boonen Kurt , Topno Rachel , Slaninova Vera , Serna Marina , Llorca Oscar , Baggerman Geert , Bertrand Edouard , West Steven , Namy Olivier , Morillon Antonin
Published: January 2024

Some long noncoding (lnc)RNAs harbor the potential to produce functional micropeptides. Despite the increasing recognition of their significance, the regulatory dynamics of cytoplasmic lncRNA expression, decay, and translation remain poorly understood. Here, we investigate the role of ribonucleases in controlling cytoplasmic levels of lncRNAs. By transcriptomic analysis we identified DIS3 but not XRN1 as a major enzyme preventing accumulation of lncRNAs in cytoplasm. Single-molecule experiments illustrate an example of DIS3-sensitive transcript (DIST) accumulation in the nucleus preceding the one in the cytoplasm, suggesting a sequential series of events. Approximately 14.5% of the DISTs contain at least one actively translated open reading frame (ORF). This finding is highly relevant to Multiple Myeloma bone marrow cancer patients’ cases with mutations impairing the DIS3 enzymatic activity and revealing a subgroup of overexpressed translatable DISTs. Immunopeptidomic approach identified the association of DIST-derived peptides with the major histocompatibility complex class I (MHCI). Notably, the low expression of DISTs in healthy tissues emphasizes their potential as targets for cancer-specific immunotherapies. Our findings shed light on the intricate regulatory mechanisms governing cytoplasmic lncRNA dynamics and highlight their clinical relevance in the context of bone marrow cancers, providing a foundation for future investigations into novel therapeutic strategies.
An Open Source R Package for Detection of Adverse Events Under-Reporting in Clinical Trials - Implementation and Validation by the IMPALA (Inter coMPany quALity Analytics) Consortium
Authors: Koneswarakantha Björn , Adyanthaya Ronojit , Emerson Jennifer , Collin Frederik , Keller Annett , Mattheus Michaela , Spyroglou Ioannis , Donevska Sandra , Ménard Timothé
Published: January 2024

Accurate and timely reporting of adverse events (AE) in clinical trials is crucial to ensuring data integrity and patient safety. However, AE under-reporting remains a challenge, often highlighted in Good Clinical Practice (GCP) audits and inspections. Traditional detection methods, such as on-site investigator audits via manual source data verification (SDV), have limitations. Addressing this, the open-source R package {simaerep} was developed to facilitate rapid, comprehensive, and near-real-time detection of AE under-reporting at each clinical trial site. This package leverages patient-level AE and visit data for its analyses. To validate its efficacy, three member companies from the Inter-coMPany quALity Analytics (IMPALA) consortium independently assessed the package. Results showed that {simaerep} consistently and effectively identified AE under-reporting across all three companies, particularly when there were significant differences in AE rates between compliant and non-compliant sites. Furthermore, {simaerep}'s detection rates surpassed heuristic methods, and it identified 50% of all detectable sites as early as 25% into the designated study duration. The open-source package can be embedded into audits to enable fast, holistic and repeatable quality oversight of clinical trials.
Novel variants in the SOX11 gene: additional evidence for the involvement of SOX11 in hypogonadotropic hypogonadism
Authors: Vieira Tarsis , Schincariol-Manhe Beatriz , Campagnolo Érica , Spineli-Silva Samira , de Leeuw Nicole , Correia-Costa Gabriela , Pessoa André , de Souza Carolina , Stevens Cathy , Javaher Poupak , Scallet Helena , Mohr Julia , Biskup Saskia , Herkert Johanna , Pfundt Rolph , Mehta Lakshmi , Rekab Aisha , Elloumi Houda , Maciel-Guerra Andréa , Lopes Vera Lucia Gil da Silva , Santos Ana dos , Sanyoura May
Published: January 2024

Pathogenic SOX11 variants have been associated with intellectual developmental disorder with microcephaly, and with or without ocular malformations or hypogonadotropic hypogonadism (IDDMOH, OMIM # 615866). In this article, we report seven new patients with SOX11 variants, five of whom have features suggestive of hypogonadotropic hypogonadism (HH). The main clinical features included neurodevelopmental delay (7/7) and intellectual disability (5/7), autism/attention deficit hyperactivity disorder (5/7), microcephaly (4/7), short stature (4/7), hypotonia (4/7), and clinodactyly of the 5th fingers (5/7). HH was confirmed in two female patients with primary amenorrhea, nonvisualized/prepubertal size of the uterus, and nonvisualized ovaries. Two of the male patients presented with micropenis, two had cryptorchidism, and one had decreased testicular size. These findings are suggestive of HH and appear to be more common than previously described among individuals with pathogenic SOX11 variants. Therefore, SOX11 should be included in diagnostic gene panels for patients with hypogonadotropic hypogonadism.
Scalable Fabrication of High-Performance Flexible Transparent Conducting Electrodes via Blade-Coating and Photonic Curing for Optoelectronic Applications
Authors: Hsu Julia , Piper Robert , Bhandari Bishal , Bonner Justin , Davis Matthew , Valdez Marisol
Published: January 2024

Flexible transparent conducting electrodes (TCEs) are critical components for many optoelectronic applications. Currently, indium tin oxide (ITO) TCEs dominate the market, but it is difficult to achieve high-performance ITO on plastic substrates. Metal nanostructures are promising alternatives for flexible TCEs because of their high transparency, excellent electrical conductance, and compatibility with roll-to-roll manufacturing. In this work, we combine flexographically printed Ag metal bus lines with a blade-coated Ag nanowire layer that is overcoated with indium zinc oxide and photonically cured to create flexible hybrid TCEs on polyethylene terephthalate (PET) substrates. Photonic curing is a promising alternative to thermal annealing as it can rapidly elevate the temperature of specific layers within microseconds or milliseconds without significantly increasing the temperature of low thermal budget plastic substrates. We chose blade-coating and photonic curing to fabricate the hybrid TCEs because both processes can be scaled to large areas. Our hybrid TCE achieves an average transmittance of 81%, an average sheet resistance of 11 Ω/sq, and a surface roughness of 4.3 nm. To evaluate the hybrid TCE performance, we fabricated halide perovskite solar cells on them and compared the results to solar cells made on commercially available PET/TCE substrates. Finally, we scaled up the blade-coating and photonic curing process from 1 in x 3 in samples to 7 in x 8 in samples to demonstrate uniform fabrication of large-area hybrid TCEs.
Cadmium toxicity to and accumulation in a soil collembolan (Folsomia candida): major factors and prediction using a back-propagation neural network mode
Authors: Li Simin , Li Zhu , Ke Xin , Wisawapipat Worachart , Christie Peter , Wu Longhua
Published: January 2024

Accurate prediction of cadmium (Cd) ecotoxicity to and accumulation in soil biota is important in soil health. However, very limited information on Cd ecotoxicity on naturally contaminated soils. Herein, we investigated soil Cd ecotoxicity using Folsomia candida, a standard single-species test animal, in 28 naturally Cd-contaminated soils, and the back-propagation neural network (BPNN) model was used to predict Cd ecotoxicity to and accumulation in F. candida. Soil total Cd and pH were the primary soil properties affecting Cd toxicity. However, soil pH was the main factor when the total Cd concentration was ˂ 3 mg kg− 1. Interestingly, correlation analysis and the K-spiked test confirmed nutrient potassium (K) was essential for Cd accumulation, highlighting the significance of studying K in Cd accumulation. The BPNN model showed greater prediction accuracy of collembolan survival rate (R2 = 0.797), reproduction inhibitory rate (R2 = 0.827), body Cd concentration (R2 = 0.961), and Cd bioaccumulation factor (R2 = 0.964) than multiple linear regression models. Then the developed BPNN model was used to predict Cd ecological risks in 57 soils in southern China. Compared to multiple linear regression models, the BPNN models can better identify high-risk regions. This study highlights the potential of BPNN as a novel and rapid tool for the evaluation and monitoring of Cd ecotoxicity in naturally contaminated soils.
Electrochemical deuterated ammonia (ND3) production at A cm-2 current densities
Authors: Zhang Zishuai , Zhu Pan , Qin Jiangzhou , Li Jun , Zuo Kuichang
Published: January 2024

Deuterated ammonia (ND3) treatment contributes significantly to the reliability of semiconductors in manufacturing. Current ND3 production suffers from high energy consumption, CO2 emissions and capital expenditure. In this study, we present an electrochemical approach using iron (Fe) single-atom catalysts supported by nitrogen-doped carbon in an electrolyzer, operating at A cm-2 current densities for ND3 production. Through systematic optimization of catalyst composition, electrode/electrolyzer architectures, anodic reactions, and operating conditions, the system achieved a ND3 selectivity exceeding 80% at 200 mA cm-2 and 60% at 600 mA cm-2, with a maximal production rate of 1.73 mmol h-1 cm-2 and stability over 100 hours (assessed at 200 mA cm-2). The full cell voltage is 3.2 V when combined with an oxygen evolution reaction (OER) anode, and it decreases to 2.3 V when coupled with a hydrogen oxidation reaction (HOR) anode at 600 mA cm-2. Techno-economic analysis (TEA) results demonstrate a fivefold energy consumption reduction to 0.19 MJ molND3-1, threefold emission reduction to 16.13 g molND3-1, and threefold cost reduction to $6,000 from $27,000 kgND3-1, highlighting the potential for widespread commercialization and contributing to the more environmentally sustainable ND3 production.
Machine Learned Interatomic Potentials for Ternary Carbides trained on the AFLOW Database
Authors: Zurek Eva , Roberts Josiah , Rijal Biswas , Divilov Simon , Maria Jon-Paul , Fahrenholtz William , Wolfe Douglas , Brenner Donald , Curtarolo Stefano
Published: January 2024

Large density functional theory (DFT) databases are a treasure trove of energies, forces and stresses that can be used to train machine learned
interatomic potentials for atomistic modeling. Herein, we employ structural relaxations from the AFLOW database to train moment tensor
potentials (MTPs) for four carbide systems: HfTaC, HfZrC, MoWC and TaTiC. The resulting MTPs are used to relax ∼6300 random symmetric
structures, and subsequently improved via active learning to generate robust potentials (RP) that can be applied to a wide variety of structures,
and accurate potentials (AP) designed only for low-energy systems. This protocol is shown to yield convex hulls that are indistinguishable
from those predicted by AFLOW for the HfTaC, HfZrC and TaTiC systems, and in the case of the CMoW system to predict thermodynamically
stable structures that are not found within AFLOW, highlighting the potential of the employed protocol within crystal structure prediction.
Relaxation of over three hundred Mo1−xWxC stoichiometry crystals first with the RP then with the AP yields formation enthalpies that are in
excellent agreement with those obtained via DFT.
Assessing the Effects of Ghost Nets on Coral Health: An Examination of Coral Reefs on Androth Island, Lakshadweep
Authors: NM Sahab , PV Ahmed Amirsha , CN Abdul Raheem , A.T Damodhar , T Aboo hashim
Published: January 2024

In our study, we investigated the impact of derelict fishing gear on coral reefs in various locations on the Androth reef of the Lakshadweep. We found significant variations in coral health across the surveyed sites, with some areas showing a high percentage of live coral while others exhibiting a high percentage of dead coral. Ghost nets snagged on reefs at all depths had a significant negative impact on coral health, according to pairwise analyses. Pearson's correlation analysis of coral health and stress relationships confirmed a significant relationship between ghost nets and coral health (LC, DC, R, and AA) at all sampled depths. In our analysis, we examined coral mortality, tissue loss, and variations in zooxanthellae density among different coral species. The average sedimentation ranges recorded monthly were 49.75 ± 43.2 and 116 ± 41.05, respectively. The sedimentation rate in the Androth reefs of the Lakshadweep was approximately five times greater than in the other regions. During our survey, we observed abandoned fishing gear, including 6 mm fishing ghost nets (28%), 5 mm fishing ghost nets (11%), ropes (31%), buoys (19%), and lines (11%) across the study sites. The overall composition of live coral cover on averaged was 22.1%, while dead coral accounted for 24.3% and rubble constituted 53.6%. Furthermore, we removed more than 48 kg of ghost nets from the NIOT underwater pipe, and significant quantities of these nets were retrieved from sites SW3 and SW2 (12.5 kg each) during our efforts. This study highlights the critical importance of addressing ghost net entanglement and its detrimental effects on coral health, as well as the urgent need for conservation efforts in the surveyed reef ecosystems.
Investigating the Effects of Monarda Didyma L. Extract Supplementation on Biological Aging of Susceptible Population: Protocol of a Randomized, Placebo- Controlled, Double-blind Parallel Clinical Trial
Authors: Campisi Manuela , Cannella Luana , Baumann Julia , Pavanello Sofia
Published: January 2024

Background
Manipulations to decelerate biological aging and extend health span are major challenges for health given the social and healthcare costs of aging population. Excessive oxidative stress and inflammation are primary drivers in biological aging and age-related diseases. Didymin, a natural bioactive flavonoid and the major polyphenol of Monarda didyma L., has showed a crucial anti-inflammatory role. This clinical study aims to investigate the potential benefits of a 12-weeks supplementation of Monarda didyma L. extract on reducing / delaying the biological aging of a susceptible population of aging workers.
Methods
This is a randomized, placebo-controlled, double-blind, parallel design and monocentric clinical trial. Study population will comprise 80 participants, aged 45–65 years, randomly allocated to both Intervention group (supplementation of Monarda didyma L. extract) and placebo control group (placebo supplementation) for a 12-weeks period of treatment. All study participants, who will be enrolled at the Occupational Medicine Unit – Azienda Ospedale Università of Padova providing a written informed consent, will undergo clinical examination, an interview with structured questionnaires (demographic data, lifestyle, information quality of life and sleeping patterns) and collection of fasting blood for evaluation of indicators of biological aging as primary outcomes (DNA methylation age, telomere length, mitochondrial DNA copy number), as well as secondary outcomes including basic biochemistry and inflammation markers (emocrome, glycemia, insulin, total cholesterol, low and high density lipoproteins, triglycerides, creatinine, telomerase expression, C-reactive protein, interleukin6, aspartate transaminase, alanine transaminase, gamma-glutamyl transferase) and salivary sample for cortisol analysis. Clinical examination and sample collection, to assess primary and secondary outcomes, will be performed at enrollment and at the follow up after of this treatment. Tracking of additional parameters (heart rate, sleep, mobility) will be performed along the study period by using a wearable MiBand 7 watch and a daily diary to determine compliance and record effects on stress, well-being and health status.
Discussion
The success of this research could represent a turning point in the healthy aging research, leading to rejuvenation by means of a sustainable and safe intervention, accessible to everybody in order to extend health span.
Trial registration:
ClinicalTrials.gov PRS (NCT05399966), registration date: May 17, 2022.
In-Depth metallurgical, design, and mechanical analysis of Reciproc Blue and four replica-like endodontic systems
Authors: Campos Gustavo Oliveira , Silva Jessica Dornelas , Buono Vicente Tadeu Lopes , Santos Leandro Arruda , Peixoto Isabella Faria da Cunha , Viana Ana Cecília Diniz
Published: January 2024

Objectives
This work aimed to evaluate the metallurgical, geometrical, and mechanical properties of 5 reciprocating endodontic files with similar cross-sectional geometry.
Materials and Methods
Reciproc Blue, ProDesign R, V File, V + File, and Univy One files were used. The evaluation of diameters was performed through high-quality photographs and Image J software. Cross-sectional scanning electron microscopy was performed in order to measure the 3 mm from the tip cross-sectional area and other aspects related to the active blade and tip. Differential scanning calorimetry tests were employed to assess the transformation temperatures and X-ray diffraction was used to identify the atomic structures. Standardized bending torsion tests were performed to evaluate the mechanical responses of the files.
Results
Significant differences were observed in the cross-sectional areas of the instruments. R-phase was predominated in most instruments, except for the Univy One system, which exhibited a combination of martensite and R-phase. The ProDesign R system demonstrated superior flexibility, while the Reciprocal Blue and V File systems exhibited greater resistance to torsion.
Conclusions
The cross-sectional area emerged as a pivotal factor influencing the mechanical characteristics of the studied files. However, the Univy One system presented high flexibility and large angular deflection despite having the highest cross-sectional area value, which suggests that the metallurgical aspects of this instrument play a major role in its mechanical behavior.
Clinical relevance:
Mechanical properties of endodontic files, such as flexibility, are very important to prevent failures during clinical practice and keep the original shape of the root canal.
Predictive modeling of molds effective elimination by external inactivation sources
Authors: Demo Pavel , Přeučil Filip , Tichá Petra , Domonkos Mária
Published: January 2024

Presented paper deals with a novel application of the (nonlinear) logistic equation to model an elimination of microscopic filaments types of fungi-molds from affected materials via different external inactivation techniques. It is shown that if the inactivation rate of the external source is greater than the maximum natural growth rate of mycelium, the mold colony becomes destroyed after a finite time. Otherwise, the mycelium may survive the external attack only at a sufficiently large initial concentration of the inoculum. Theoretically determined growth curves are compared with the experimental data for Aspergillus brasiliensis mold inactivated by using both cold atmospheric plasma (CAP) and UV-germicidal lamp. Model presented in the article may be applied also to other classes of microorganisms (e.g. bacteria).
Clinical risk factors and protective factors of preeclampsia in China
Authors: Hou Lei , Li Jieyan , Zhao Rong , Liu Liheng , Zhang Weiyuan , Zou Liying
Published: January 2024

Objective: The pathophysiology of preeclampsia remains unclear. Identification of risk factors and protective factors will help us to better elucidate the pathophysiologic basis of preeclampsia and predict it.
Methods: A cross sectional survey of all deliveries in 39 hospitals in 14 provinces of mainland China from January 1 to December 31 2011 was evaluated for risk factors and protective factors of preeclampsia.
Results: The incidence of preeclampsia was 3.7% (4137/110926), The results of multivariate logistic analysis showed that the main independent risk factors for preeclampsia included chronic hypertension[RR=9.190, 95%CI: (8.102-10.423)], assisted reproduction [OR=4.487, 95%CI:(2.591-7.772)], obesity [OR=3.822 , 95%CI: (3.556-4.107)], advanced age [OR=2.509(2.321-2.712)], GDM/DM [OR=1.656(1.479-1.855)], multiple pregnancy [OR=1.491, 95%CI: (1.283-1.735)], anemia [OR=1.253, 95%CI: (1.110-1.414)] and AB blood type [OR=1.131 , 95%CI: (1.008-1.269)]. Protective factors for preeclampsia included placenta previa [OR=0.401, 95%CI: (0.296-0.543)], A blood type [OR=0.885 , 95%CI: (0.816-0.959)] and height [OR=0.991, 95%CI: (0.985-0.997)]. The obstetrics complications associated with preeclampsia raised obviously, including preterm birth[RR=8.542 , 95%CI: (7.985-9.137)], FGR[RR= 5.020 , 95%CI: (4.635-5.437)], placenta abruption [RR=3.453, 95%CI: (2.777-4.293)]and oligohydramnios [RR=1.520, 95%CI: (1.306-1.768)].
Discussion: Our findings suggest chronic hypertension, assisted reproduction, obesity, advanced maternal age, GDM/DM, multiple pregnancy, anemia and AB blood type were the risk factors of preeclampsia. placenta previa, A blood type, height, and advanced educated degree were the protective factors for it.
Visual Analysis Method for Unmanned Pumping Stations on Dynamic Platforms based on Data Fusion Technology
Authors: Liu Zhen , Chen Sen , Zhang Zhaobo , Qin Jiahao , Peng Bao
Published: January 2024

As the scale of water conservancy projects continues to expand, the amount and complexity of analytical data have also correspondingly increased. Currently, As the scale of water conservancy projects continues to expand, the amount and complexity of analytical data have also correspondingly increased. Currently, It is difficult to realize project management decision support based on a single data source, and most manual analysis methods not only have high labor costs, but also are prone to the risk of misjudgment and misjudgement, resulting in huge property losses. Based on this problem, this paper proposes a visualization and analysis method for unmanned pumping stations on a dynamic platform based on data fusion technology. First, the method uses the transfer learning method to make ResNet18 model obtain generalization ability. Secondly, the method uses the ResNet18 model to extract image features, and outputs fixed length sequence data as the input of long short-term memory (LSTM) model, Finally, the method uses the LSTM model outputs the classification results. The experimental results show that the algorithm model can achieve an accuracy of 99.032%, effectively recognize and classify pump station images, and reduce the occurrence of pump station accidents.
Anti-biofilm activity of antimicrobial peptide CRAMP-34 by regulating 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (KduD) of Escherichia coli
Authors: Xiong Jing , Yang Can , Liu Dan , Yang Hongzao , Long Jinying , Liang Yuan , Hu Huiming , Peng Lianci , Zhang Yang , Fu Lizhi , Yu Yuandi , Xu Dengfeng , Zhang Suhui , Qiu Jinjie , He Yuzhang , Chen Hongwei
Published: January 2024

Escherichia coli (E. coli) has high antimicrobial resistance and is globally prevalent, forming aggressive and dense bacterial biofilms. Previous studies have shown that the mouse antimicrobial peptide CRAMP-34 eradicates biofilms. The current study aimed to investigate the mechanism of CRAMP-34 on the biofilm of a clinically isolated E. coli (No. Ec032) with strong biofilm formation ability. An unreported gene kduD associated with Ec032 biofilm formation was identified by transposon mutation technology, and the kduD gene deletion strain Ec032ΔkduD was constructed using CRISPR/Cas9. Furthermore, the deletion of kduD gene reduced the motility of Ec032 and inhibited the aggregation of bacteria to form mature biofilms. Also, CRAMP-34 inhibited the motility of Ec032 and significantly cleared the mature biofilm. RT-qPCR showed that CRAMP-34 significantly downregulated the biofilm-related genes, including ycgR, papG, csgD, bcsA, uxaA, uxuA, kduD, and araE. These findings indicated that KduD protein is a potential target of CRAMP-34. Therefore, this study provides the foundation for the prevention and treatment of E. coli biofilm and also develops CRAMP-34 as a new biofilm scavenger.