Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system
Authors: Wahjuningrum Dinamella , Efianda Teuku Reza , Tarman Kustiariyah , Yuhana Munti , Effendi Irzal , Saputra Fazril 
Published: September 2020

ABSTRACT
The purpose of this study is to evaluate the effectiveness of Nodulisporium sp. KT29 supplementation with variousVibrio harveyi induction in feed against vibriosis in vannamei white shrimp. The study design included KP (positivecontrol without supplementation Nodulisporium sp. KT29 and infected with V. harveyi), KN (negative controlswithout Nodulisporium sp. KT29 and infected with physiological solution), NT (treatment of supplementationNodulisporium sp KT29 20 mL/kg and infected with V. harveyi), NM (treatment of supplementation testNodulisporium sp. KT29 induction of V. harveyi dead cell 20 mL/kg and infected V. harveyi), NH (treatment ofsupplementation Nodulisporium sp. KT29 induction of V. harveyi live cell 20 mL/kg and infected with V. harveyi).The study parameters included inhibition zone, resistance, immune responses, and hemolim glucose. The resultsshowed Nodulisporium sp. KT29 with induction treatment raised antibacterial activity with best treatment of NMand NH (P<0.05). The results of V. harveyi infection resistance presented NM treatment of 20 mL/kg increasesurvival in vannamei shrimp reached 72.2% (P<0.05). In addition, the same treatment increase the immuneresponse activity and decrease the activity of hemolim glucose. It could be concluded that providing NM 20 mLtreatment boosted the resistance and the immune system in vaname shrimp to control vibriosis reared at the sea.Keywords: antibacterial, β-glucan, induced, Nodulisporium sp. KT29, Vibrio harveyi
ABSTRAK
Tujuan penelitian yaitu mengevaluasi efektivitas suplementasi Nodulisporium sp. KT29 dengan berbagai perlakuaninduksi Vibrio harveyi dalam pakan terhadap pengendalian vibriosis pada udang vaname yang dibudidayakan dilaut. Rancangan penelitian meliputi KP (kontrol positif tanpa suplementasi Nodulisporium sp. KT29 dan diinfeksiV. harveyi), KN (kontrol negatif tanpa Nodulisporium sp. KT29 dan diinfeksi larutan fisiologis), NT (perlakuanuji suplementasi Nodulisporium sp. KT29 20 mL/kg dan diinfeksi V. harveyi), NM (perlakuan uji suplementasiNodulisporium sp. KT29 diinduksi sel mati V. harveyi 20 mL/kg dan diinfeksi V. harveyi), NH (perlakuan ujisuplementasi Nodulisporium sp. KT29 induksi sel hidup V. harveyi 20 mL/kg dan diinfeksi V. harveyi). Parameterpenelitian meliputi zona hambat, resistensi, respons imun, dan glukosa hemolim. Hasil penelitian menunjukkanNodulisporium sp. KT29 dengan perlakuan induksi dapat meningkatkan aktivitas antibakteri dengan perlakuanterbaik NM dan NH (P<0.05). Hasil pengamatan resistensi infeksi V. harveyi menunjukkan perlakuan NM 20 mL/kg dapat meningkatkan kelangsungan hidup pada udang vaname mencapai 72.2% (P<0.05). Perlakuan yang samajuga meningkatkan respons imun dan menurunkan aktivitas glukosa hemolim. Disimpulkan bahwa pemberianNM 20 mL dapat meningkatkan resistensi dan sistem imun udang vaname terhadap pengendalian vibriosis di laut.Kata kunci : antibakteri, β-glucan, induksi, Nodulisporium sp. KT29, Vibrio harveyi,