bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Meteorological Conditions on Riverbed Dust Aerosol in Taiwan
Authors: Tsai Fujung , Yao Wan-Chi , Lin Ming-Lung
Published: January 2022

Extremely high concentrations of dust particles are occasionally generated from the riverbeds of Taiwan, affecting the visibility and traffic safety of the local and nearby areas. The condition is most severe during the winter monsoon when surface wind is strong. This study analyzes the concentration of particulate matter of 10 µm or less (PM10), wind direction, wind speed, temperature, and humidity of riverbed stations adjacent to the Daan, Dajia, Dadu, Zhuoshui, and Beinan Rivers in Taiwan for a period of two years. The weather conditions that cause the high concentration of PM10 are classified into typhoon and non-typhoon types, and the latter type is further classified into three stages: ahead of front, ahead of anticyclone, and behind anticyclone. The associated meteorological influences of these weather types on high-concentration events in the riverbed are explored. The monitoring data show that the hourly PM10 concentration of the four riverbed stations exceeded 125 µg m−3 for 35–465 h per year, and the maximum PM10 in the Daan (and Dajia), and Zhuoshui Rivers was more than 800 µg m−3. Weather analysis showed that the extreme PM10 concentration on the riverbed was caused by weather types: typhoon and ahead of anticyclone, in which the peak hourly concentration reached average values of more than 600 and 400 µg m−3, respectively. The high PM10 caused by the typhoon type mainly occurred in October, with an average wind speed of 6 m s−1, high temperature of 25 °C, and mostly northeasterly winds. The ahead of anticyclone type mainly occurred in December, with an average wind speed of 5 m s−1, and northeasterly and northwesterly winds. Both weather types of riverbed events were observed during the daytime, especially at noon time, when strong wind speed, high temperature, and low relative humidity is favorable for riverbed dust generation. On the other hand, the main months of the high PM10 concentrations of the ahead of front and behind anticyclone stages are February and April. The peak PM10 concentrations of these two types of riverbed events are both about 300 µg m−3, but sporadic riverbed dust in these weather stages is mixed with Asian dust or pollution transported to the rivers through weak northwesterly and northeasterly winds. The high concentrations of these two types of riverbed events can occur at any time; but for the Dadu River, the high concentrations are often observed in the morning, when land breezes from the southeast bring local pollutants to the river.
WRF Sensitivity for Seasonal Climate Simulations of Precipitation Fields on the CORDEX South America Domain
Authors: Gomes Helber Barros , Lemos da Silva Maria Cristina , Barbosa Henrique de Melo Jorge , Ambrizzi Tércio , Baltaci Hakki , Gomes Heliofábio Barros , Silva Fabrício Daniel dos Santos , Costa Rafaela Lisboa , Figueroa Silvio Nilo , Herdies Dirceu Luis , Pauliquevis Júnior Theotonio Mendes
Published: January 2022

Dynamic numerical models of the atmosphere are the main tools used for weather and climate forecasting as well as climate projections. Thus, this work evaluated the systematic errors and areas with large uncertainties in precipitation over the South American continent (SAC) based on regional climate simulations with the weather research and forecasting (WRF) model. Ten simulations using different convective, radiation, and microphysical schemes, and an ensemble mean among them, were performed with a resolution of 50 km, covering the CORDEX-South America domain. First, the seasonal precipitation variability and its differences were discussed. Then, its annual cycle was investigated through nine sub-domains on the SAC (AMZN, AMZS, NEBN, NEBS, SE, SURU, CHAC, PEQU, and TOTL). The Taylor Diagrams were used to assess the sensitivity of the model to different parameterizations and its ability to reproduce the simulated precipitation patterns. The results showed that the WRF simulations were better than the ERA-interim (ERAI) reanalysis when compared to the TRMM, showing the added value of dynamic downscaling. For all sub-domains the best result was obtained with the ensemble compared to the satellite TRMM. The largest errors were observed in the SURU and CHAC regions, and with the greatest dispersion of members during the rainy season. On the other hand, the best results were found in the AMZS, NEBS, and TOTL regions.
Anti-Planktonic and Anti-Biofilm Properties of Pentacyclic Triterpenes—Asiatic Acid and Ursolic Acid as Promising Antibacterial Future Pharmaceuticals
Authors: Sycz Zuzanna , Tichaczek-Goska Dorota , Wojnicz Dorota
Published: January 2022

Due to the ever-increasing number of multidrug-resistant bacteria, research concerning plant-derived compounds with antimicrobial mechanisms of action has been conducted. Pentacyclic triterpenes, which have a broad spectrum of medicinal properties, are one of such groups. Asiatic acid (AA) and ursolic acid (UA), which belong to this group, exhibit diverse biological activities that include antioxidant, anti-inflammatory, diuretic, and immunostimulatory. Some of these articles usually contain only a short section describing the antibacterial effects of AA or UA. Therefore, our review article aims to provide the reader with a broader understanding of the activity of these acids against pathogenic bacteria. The bacteria in the human body can live in the planktonic form and create a biofilm structure. Therefore, we found it valuable to present the action of AA and UA on both planktonic and biofilm cultures. The article also presents mechanisms of the biological activity of these substances against microorganisms.
Editorial of Special Issue “Fibrodysplasia Ossificans Progressiva: Studies on Disease Mechanism towards Novel Therapeutic Approaches”
Authors: Ravazzolo Roberto
Published: January 2022

The Special Issue on “Fibrodysplasia Ossificans Progressiva: Studies on Disease Mechanism towards Novel Therapeutic Approaches” has published interesting and useful review articles and original experimental articles on fibrodysplasia ossificans progressiva (FOP), a very rare genetic disorder for which much effort is being devoted to search for a cure. In this editorial, I briefly cite the essential content of all the published articles.
Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors
Authors: Nikelshparg Evelina I. , Baizhumanov Adil A. , Bochkova Zhanna V. , Novikov Sergey M. , Yakubovsky Dmitry I. , Arsenin Aleksey V. , Volkov Valentyn S. , Goodilin Eugene A. , Semenova Anna A. , Sosnovtseva Olga , Maksimov Georgy V. , Brazhe Nadezda A.
Published: January 2022

Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.
Pain-Related Abnormal Neuronal Synchronization of the Nucleus Accumbens in Parkinson’s Disease
Authors: Kinugawa Kaoru , Mano Tomoo , Yamatani Yuya , Miyasaka Toshiteru , Kataoka Hiroshi , Sugie Kazuma
Published: January 2022

Patients with Parkinson’s disease (PD) often experience pain, which fluctuates in “on” and “off” states, but the underlying mechanism is unclear. The nucleus accumbens (NAc) is a central component of the mesolimbic dopaminergic pathway involved in pain processing. We conducted resting-state functional magnetic resonance imaging (rsfMRI) analysis to explore the relationship between the neuronal synchronization of NAc with pain-related brain regions and pain intensity in “on” and “off” states. We assessed 23 patients with sporadic PD based on rsfMRI and pain intensity using the revised Short-Form McGill Pain Questionnaire. Patients with PD displayed higher pain intensity scores in the “off” state than in the “on” state. The pain intensity in the “off” state was substantially correlated with the functional connectivity (FC) between the NAc and primary motor/sensory cortices and contralateral NAc. Changes in pain intensity from the “on” to “off” state displayed correlations with those between the right (rNA) and left NAc (lNAc) and the right precentral gyrus (rPreCG) /right insular cortex (rIC) from the “off” to “on” state. Aberrant bilateral NAc and rNAc–rPreCG/rIC FC in the “off” state were closely related to pain symptoms developed from the “on” to “off” states. These results suggest that the NAc in the mesolimbic pathway is related to pain in PD and may help understand the mechanism of pain development in patients with PD.
The Physical and Mechanical Properties of Autoclaved Aerated Concrete (AAC) with Recycled AAC as a Partial Replacement for Sand
Authors: Rafiza Abdul Rahman , Fazlizan Ahmad , Thongtha Atthakorn , Asim Nilofar , Noorashikin Md Saleh
Published: January 2022

The application of AAC has increased considerably in Malaysia since the 1990s. The usage of AAC has some advantages, but it also has negative environmental impacts since rejected concrete will become landfill. This study aimed to use AAC waste powder as a material that would partially replace the sand content to produce a new form of Autoclaved Aerated Concrete (AAC). The physical and mechanical properties of the newly developed AAC were investigated. This paper presents improved mechanical and physical properties of the new form of recycled AAC concrete. Besides these improvements, using recycled AAC could lower production costs. Furthermore, the usage of this recycled waste powder is both economically and environmentally advantageous. This study found that when recycled AAC was substituted for sand, AAC with a fine recycled powder content of 30% had a compressive strength that was around 16% higher than conventional AAC and between 29% and 156% higher than any value attained utilizing an industrial waste product. This study also confirmed that the greater strength could be identical to a higher tobermorite phase and that the recycled AAC surface showed a finer crystalline morphology.
Mechanical Performance Prediction for Sustainable High-Strength Concrete Using Bio-Inspired Neural Network
Authors: Sun Junbo , Wang Jiaqing , Zhu Zhaoyue , He Rui , Peng Cheng , Zhang Chao , Huang Jizhuo , Wang Yufei , Wang Xiangyu
Published: January 2022

High-strength concrete (HSC) is a functional material possessing superior mechanical performance and considerable durability, which has been widely used in long-span bridges and high-rise buildings. Unconfined compressive strength (UCS) is one of the most crucial parameters for evaluating HSC performance. Previously, the mix design of HSC is based on the laboratory test results which is time and money consuming. Nowadays, the UCS can be predicted based on the existing database to guide the mix design with the development of machine learning (ML) such as back-propagation neural network (BPNN). However, the BPNN’s hyperparameters (the number of hidden layers, the number of neurons in each layer), which is commonly adjusted by the traditional trial and error method, usually influence the prediction accuracy. Therefore, in this study, BPNN is utilised to predict the UCS of HSC with the hyperparameters tuned by a bio-inspired beetle antennae search (BAS) algorithm. The database is established based on the results of 324 HSC samples from previous literature. The established BAS-BPNN model possesses excellent prediction reliability and accuracy as shown in the high correlation coefficient (R = 0.9893) and low Root-mean-square error (RMSE = 1.5158 MPa). By introducing the BAS algorithm, the prediction process can be totally automatical since the optimal hyperparameters of BPNN are obtained automatically. The established BPNN model has the benefit of being applied in practice to support the HSC mix design. In addition, sensitivity analysis is conducted to investigate the significance of input variables. Cement content is proved to influence the UCS most significantly while superplasticizer content has the least significance. However, owing to the dataset limitation and limited performance of ML models which affect the UCS prediction accuracy, further data collection and model update must be implemented.
Prevalence and Management of Cancer of the Rectal Stump after Total Colectomy and Rectal Sparing in Patients with Familial Polyposis: Results from a Registry-Based Study
Authors: Colletti Gaia , Ciniselli Chiara Maura , Signoroni Stefano , Cocco Ivana Maria Francesca , Magarotto Andrea , Ricci Maria Teresa , Brignola Clorinda , Bagatin Clara , Cattaneo Laura , Mancini Andrea , Cavalcoli Federica , Milione Massimo , Verderio Paolo , Vitellaro Marco
Published: January 2022

Background: The balance between quality of life and colorectal cancer risk in familial adenomatous polyposis (FAP) patients is of primary importance. A cut-off of less than 30 polyps under 1 cm of diameter in the rectum has been used as an indication for performing ileo-rectal anastomosis (IRA) in terms of lower rectal cancer risk. This study aimed to assess clinical and surgical features of FAP patients who developed cancer of the rectal stump. Methods: This retrospective study included all FAP patients who underwent total colectomy/IRA from 1977 to 2021 and developed subsequent rectal cancer. Patients’ features were reported using descriptive statistics by considering the overall case series and within pre-specified classes of age (<20, 20–30, and >30 years) at first surgery. Results: Among the 715 FAP patients, 47 (6.57%, 95% confidence interval: 4.87; 8.65) developed cancer in the rectal stump during follow-up. In total, 57.45% of the population were male and 38.30% were proband. The median interval between surgery and the occurrence of rectal cancer was 13 years. This interval was wider in the youngest group (p-value: 0.012) than the oldest ones. Twelve patients (25.53%) received an endoscopic or minimally invasive resection. Amongst them, 61.70% were Dukes stage A cancers. Conclusions: There is a definite risk of rectal cancer after total colectomy/IRA; however, the time interval from the index procedure to cancer developing is long. Minimally invasive and endoscopic treatments should be the procedures of choice in patients with early stage cancers.
Imaging Immune Cells Using Fc Domain Probes in Mouse Cancer Xenograft Models
Authors: Bernhard Wendy , Barreto Kris , El-Sayed Ayman , DeCoteau John , Geyer C. Ronald
Published: January 2022

Tracking immune responses is complex due to the mixture of cell types, variability in cell populations, and the dynamic environment. Tissue biopsies and blood analysis can identify infiltrating and circulating immune cells; however, due to the dynamic nature of the immune response, these are prone to sampling errors. Non-invasive targeted molecular imaging provides a method to monitor immune response, which has advantages of providing whole-body images, being non-invasive, and allowing longitudinal monitoring. Three non-specific Fc-containing proteins were labeled with near-infrared dye IRDye800CW and used as imaging probes to assess tumor-infiltrating immune cells in FaDu and A-431 xenograft models. We showed that Fc domains localize to tumors and are visible by fluorescent imaging. This tumor localization appears to be based on binding tumor-associated immune cells and some xenografts showed higher fluorescent signals than others. The Fc domain alone bound to different human immune cell types. The Fc domain can be a valuable research tool to study innate immune response.
Stereotactic Radiation and Dual Human Epidermal Growth Factor Receptor 2 Blockade with Trastuzumab and Pertuzumab in the Treatment of Breast Cancer Brain Metastases: A Single Institution Series
Authors: Ippolito Edy , Silipigni Sonia , Matteucci Paolo , Greco Carlo , Pantano Francesco , D’Auria Giuliana , Quattrocchi Carlo Cosimo , Floreno Barnaba , Fiore Michele , Gamucci Teresa , Tonini Giuseppe , Ramella Sara
Published: January 2022

(1) Background: This study aims to assess the safety and efficacy of fractionated SRT (fSRT) and pertuzumab–trastuzumab (PT) in patients with breast cancer brain metastases (BCBM). (2) Methods: Patients with HER2+ BCBM who received FSRT from 2015 to 2019 were identified. Patients were included if they were treated with fSRT within 21 days of receiving PT. All lesions were treated with LINAC-based fSRT to a total dose of 27 Gy delivered in three consecutive fractions. All patients received concurrent PT. Patients were evaluated 4–6 weeks after SRS and subsequently every 2–3 months with MRI re-imaging (3) Results: A total of 49 patients with HER2+ brain metastases were identified. Of these patients, a total of 10 patients with 32 HER2+ BCBM were treated with concurrent SRT and PT and included in the analysis. No local progression was observed. Overall response rate was 68.7%. Only one patient developed asymptomatic radionecrosis. Median time to BM occurrence was 15.6 (range: 1–40.5 months). Distant intracranial failure occurred in 4/10 patients (40.0%). Overall BCBM median survival was 33.9 months (95%CI 24.1–43.6). Mean duration of PT treatment was 27.9 months (range: 10.1–53.7 months). (4) Conclusions: In our single institution experience, fSRT and PT showed to be a safe treatment for patients with BCBM with an adequate overall response rate.
PRMT1 Regulates EGFR and Wnt Signaling Pathways and Is a Promising Target for Combinatorial Treatment of Breast Cancer
Authors: Suresh Samyuktha , Huard Solène , Brisson Amélie , Némati Fariba , Dakroub Rayan , Poulard Coralie , Ye Mengliang , Martel Elise , Reyes Cécile , Silvestre David C. , Meseure Didier , Nicolas André , Gentien David , Fayyad-Kazan Hussein , Le Romancer Muriel , Decaudin Didier , Roman-Roman Sergio , Dubois Thierry
Published: January 2022

Identifying new therapeutic strategies for triple-negative breast cancer (TNBC) patients is a priority as these patients are highly prone to relapse after chemotherapy. Here, we found that protein arginine methyltransferase 1 (PRMT1) is highly expressed in all breast cancer subtypes. PRMT1 depletion decreases cell survival by inducing DNA damage and apoptosis in various breast cancer cell lines. Transcriptomic analysis and chromatin immunoprecipitation revealed that PRMT1 regulates the epidermal growth factor receptor (EGFR) and the Wnt signaling pathways, reported to be activated in TNBC. PRMT1 enzymatic activity is also required to stimulate the canonical Wnt pathway. Type I PRMT inhibitors decrease breast cancer cell proliferation and show anti-tumor activity in a TNBC xenograft model. These inhibitors display synergistic interactions with some chemotherapies used to treat TNBC patients as well as erlotinib, an EGFR inhibitor. Therefore, targeting PRMT1 in combination with these chemotherapies may improve existing treatments for TNBC patients.
Nicotinamide Inhibits T Cell Exhaustion and Increases Differentiation of CD8 Effector T Cells
Authors: Alavi Sara , Emran Abdullah Al , Tseng Hsin-Yi , Tiffen Jessamy C. , McGuire Helen Marie , Hersey Peter
Published: January 2022

One of the limitations of immunotherapy is the development of a state referred to as T cell exhaustion (TEx) whereby T cells express inhibitory receptors (IRs) and lose production of effectors involved in killing of their targets. In the present studies we have used the repeated stimulation model with anti CD3 and anti CD28 to understand the factors involved in TEx development and treatments that may reduce changes of TEx. The results show that addition of nicotinamide (NAM) involved in energy supply to cells prevented the development of inhibitory receptors (IRs). This was particularly evident for the IRs CD39, TIM3, and to a lesser extent LAG3 and PD1 expression. NAM also prevented the inhibition of IL-2 and TNFα expression in TEx and induced differentiation of CD4+ and CD8 T cells to effector memory and terminal effector T cells. The present results showed that effects of NAM were linked to regulation of reactive oxygen species (ROS) consistent with previous studies implicating ROS in upregulation of TOX transcription factors that induce TEx. These effects of NAM in reducing changes of TEx and in increasing the differentiation of T cells to effector states appears to have important implications for the use of NAM supplements in immunotherapy against cancers and viral infections and require further exploration in vivo.
Two Doses of BNT162b2 mRNA Vaccine in Patients after Hematopoietic Stem Cell Transplantation: Humoral Response and Serological Conversion Predictors
Authors: Majcherek Maciej , Matkowska-Kocjan Agnieszka , Szymczak Donata , Karasek Magdalena , Szeremet Agnieszka , Kiraga Aleksandra , Milanowska Aneta , Kuznik Edwin , Kujawa Krzysztof , Wrobel Tomasz , Szenborn Leszek , Czyz Anna
Published: January 2022

Vaccination against SARS-CoV-2 is currently the best tool in the fight against the COVID-19 pandemic. However, there are limited data on its efficacy and safety after hematopoietic stem cell transplantation (HCT). We present the results of a prospective analysis of the humoral response to two doses of BNT162b2 mRNA vaccine in 93 adult patients, including 29 after autologous HCT (autoHCT) and 64 after allogeneic HCT (alloHCT). Positive anti-SARS-CoV-2 antibodies were detected before vaccination in 25% of patients despite a negative medical history of COVID-19. Seroconversion after vaccination was achieved in 89% of patients after alloHCT and in 96% after autoHCT, without grade 3/4 adverse events. Post-vaccination anti-SARS-CoV-2 antibody level correlated with the time from transplant and absolute B-cell count at the vaccination. In univariate analysis restricted to the alloHCT group, short time since transplantation, low B-cell count, low intensity conditioning, GvHD, and immunosuppressive treatment at the vaccination were associated with lack of seroconversion. In the multivariate model, the only negative predictor of seroconversion remained treatment with calcineurin inhibitor (CNI). In conclusion, the BNT162b2 mRNA vaccine is highly immunogenic in patients after HCT, but treatment with CNI at the time of vaccination has a strong negative impact on the humoral response
Association of Late Marriage and Low Childbirth with Cervical Cancer Screening among Korean Women: Results from a Nationwide Survey
Authors: Shin Hye Young , Park Bomi , Suh Mina , Choi Kui Son , Jun Jae Kwan
Published: January 2022

This study aimed to identify the association of marriage and childbirth with the adherence to cervical cancer screening among young adult women. Data across four years (2017–2020) of the cross-sectional Korean National Cancer Screening Survey were used. For measuring the adherence to cervical cancer screening, we used the cervical cancer screening rate with recommendation, which was defined as the percentage of women in the population eligible for screening who have had a cervical cancer screening within the past two years. Multiple logistic regression analysis was conducted to identify the association between marriage and adherence to cervical cancer screening. Overall, 3925 women aged 20–39 years were analyzed. Of these, 39.1% were screened for cervical cancer (26.6% unmarried and 57.1% married women). The married women had significantly higher adherence to cervical cancer screening than unmarried women (adjusted odds ratio = 2.80, 95% CI: 2.99–3.44). Compared with unmarried women, adherence to cervical cancer screening was significantly more likely to increase (p for trend, <0.001) in married women with an increased number of births. Our study confirmed that marriage and childbirth influence adherence to cervical cancer screening, suggesting that unmarried women may be vulnerable to cervical cancer.
Challenging Conditions for Gasoline Particulate Filters (GPFs)
Authors: Giechaskiel Barouch , Melas Anastasios , Valverde Victor , Otura Marcos , Martini Giorgio
Published: January 2022

The emission limit of non-volatile particles (i.e., particles that do not evaporate at 350 °C) with size >23 nm, in combination with the real driving emissions (RDE) regulation in 2017, resulted in the introduction of gasoline particulate filters (GPFs) in all light-duty vehicles with gasoline direct injection engines in Europe. Even though there are studies that have examined the particulate emissions at or beyond the current RDE boundary conditions, there is a lack of studies combining most or all worst cases (i.e., conditions that increase the emissions). In this study, we challenged a fresh (i.e., no accumulation of soot or ash) “advanced” prototype GPF at different temperatures (down to −9 °C), aggressive drive cycles and hard accelerations (beyond the RDE limits), high payload (up to 90%), use of all auxiliaries (air conditioning, heating of the seats and the rear window), and cold starts independently or simultaneously. Under hot engine conditions, the increase of the particulate emissions due to higher payload and lower ambient temperature was 30–90%. The cold start at low ambient temperature, however, had an effect on the emissions of up to a factor of 20 for particles >23 nm or 300 when considering particles <23 nm. We proposed that the reason for these high emissions was the incomplete combustion and the low efficiency of the three-way oxidation catalyst. This resulted in a high concentration of species that were in the gaseous phase at the high temperature of the close-coupled GPF and thus could not be filtered by the GPF. As the exhaust gas cooled down, these precursor species formed particles that could not be evaporated at 350 °C (the temperature of the particle number system). These results highlight the importance of the proper calibration of the engine out emissions at all conditions, even when a GPF is installed.
Glycerol Hydrogenolysis to 1,2-Propanediol over Novel Cu/ZrO2 Catalysts
Authors: Luciani Giuseppina , Ruoppolo Giovanna , Landi Gianluca , Gargiulo Valentina , Alfè Michela , Di Benedetto Almerinda
Published: January 2022

Glycerol is the main by-product of biodiesel production; its upgrading to more valuable products is a demanding issue. Hydrogenolysis to 1,2-propanediol is one of the most interesting processes among the possible upgrading routes. In this study, we propose novel copper/zirconia catalysts prepared by advanced preparation methods, including copper deposition via metal–organic framework (MOF) and support preparation via the sol–gel route. The catalysts were characterized by N2 physisorption, X-ray diffraction, Scanning Electron Microscopy, H2-TPR and NH3-TPD analyses and tested in a commercial batch reactor. The catalyst prepared by copper deposition via MOF decomposition onto commercial zirconia showed the best catalytic performance, reaching 75% yield. The improved catalytic performance was assigned to a proper combination of redox and acid properties. In particular, a non-negligible fraction of cuprous oxide and of weak acid sites seems fundamental to preferentially activate the selective pathway. In particular, these features avoid the overhydrogenolysis of 1,2-propanediol to 1-propanol and enhance glycerol dehydration to hydroxyacetone and the successive hydrogenation of hydroxyacetone to 1,2-propanediol.
Mitophagy Regulation Following Myocardial Infarction
Authors: Turkieh Annie , El Masri Yara , Pinet Florence , Dubois-Deruy Emilie
Published: January 2022

Mitophagy, which mediates the selective elimination of dysfunctional mitochondria, is essential for cardiac homeostasis. Mitophagy is regulated mainly by PTEN-induced putative kinase protein-1 (PINK1)/parkin pathway but also by FUN14 domain-containing 1 (FUNDC1) or Bcl2 interacting protein 3 (BNIP3) and BNIP3-like (BNIP3L/NIX) pathways. Several studies have shown that dysregulated mitophagy is involved in cardiac dysfunction induced by aging, aortic stenosis, myocardial infarction or diabetes. The cardioprotective role of mitophagy is well described, whereas excessive mitophagy could contribute to cell death and cardiac dysfunction. In this review, we summarize the mechanisms involved in the regulation of cardiac mitophagy and its role in physiological condition. We focused on cardiac mitophagy during and following myocardial infarction by highlighting the role and the regulation of PI NK1/parkin-; FUNDC1-; BNIP3- and BNIP3L/NIX-induced mitophagy during ischemia and reperfusion.
Human iPSC-Cardiomyocytes as an Experimental Model to Study Epigenetic Modifiers of Electrophysiology
Authors: Pozo Maria R. , Meredith Gantt W. , Entcheva Emilia
Published: January 2022

The epigenetic landscape and the responses to pharmacological epigenetic regulators in each human are unique. Classes of epigenetic writers and erasers, such as histone acetyltransferases, HATs, and histone deacetylases, HDACs, control DNA acetylation/deacetylation and chromatin accessibility, thus exerting transcriptional control in a tissue- and person-specific manner. Rapid development of novel pharmacological agents in clinical testing—HDAC inhibitors (HDACi)—targets these master regulators as common means of therapeutic intervention in cancer and immune diseases. The action of these epigenetic modulators is much less explored for cardiac tissue, yet all new drugs need to be tested for cardiotoxicity. To advance our understanding of chromatin regulation in the heart, and specifically how modulation of DNA acetylation state may affect functional electrophysiological responses, human-induced pluripotent stem-cell-derived cardiomyocyte (hiPSC-CM) technology can be leveraged as a scalable, high-throughput platform with ability to provide patient-specific insights. This review covers relevant background on the known roles of HATs and HDACs in the heart, the current state of HDACi development, applications, and any adverse cardiac events; it also summarizes relevant differential gene expression data for the adult human heart vs. hiPSC-CMs along with initial transcriptional and functional results from using this new experimental platform to yield insights on epigenetic control of the heart. We focus on the multitude of methodologies and workflows needed to quantify responses to HDACis in hiPSC-CMs. This overview can help highlight the power and the limitations of hiPSC-CMs as a scalable experimental model in capturing epigenetic responses relevant to the human heart.
Raltegravir Inclusion Decreases CD4 T-Cells Intra-Cellular Viral Load and Increases CD4 and CD28 Positive T-Cells in Selected HIV Patients
Authors: Kumar Gaurav , Cottalorda-Dufayard Jacqueline , Garraffo Rodolphe , De Salvador-Guillouët Francine , Cua Eric , Roger Pierre-Marie
Published: January 2022

Raltegravir (RLT) prevents the integration of HIV DNA in the nucleus, but published studies remain controversial, suggesting that it does not decrease proviral DNA. However, there are only a few studies focused on virus-targeted cells. We aimed our study on the impact of RLT inclusion on total intra-cellular viral DNA (TID) in cellular subsets and immune effects in patients with newly acquired undetectable plasmatic viral load (UVL). Six patients having UVL using an antiretroviral combination for 6 months and CD4 T-cells > 350/mL and <500/mL were selected to receive RLT for 3 months from M0 to M3. Patients had 7 sequential viro-immunological determinations from M-1 to M5. Immune phenotypes were determined by flow cytometry and TID quantification was performed using PCR assay on purified cells. TID (median values) at the initiation of RLT in CD4 T-cells was 117 copies/millions of cells, decreased to 27.5 on M3, and remained thereafter permanently under the cut-off (<10 copies/millions of cells) in 4 out of 6 patients. This was associated with an increase of CD4 and CD4 + CD28+ T-cells and a decrease of HLA-DR expression and apoptosis of CD4 T-cells. RLT inclusion led to decreases in the viral load along with positive immune reconstitution, mainly for CD4 T-cells in HIV patients.
Biomarkers-in-Cardiology 8 RE-VISITED—Consistent Safety of Early Discharge with a Dual Marker Strategy Combining a Normal hs-cTnT with a Normal Copeptin in Low-to-Intermediate Risk Patients with Suspected Acute Coronary Syndrome—A Secondary Analysis of the Randomized Biomarkers-in-Cardiology 8 Trial
Authors: Giannitsis Evangelos , Garfias-Veitl Tania , Slagman Anna , Searle Julia , Müller Christian , Blankenberg Stefan , von Haehling Stephan , Katus Hugo A. , Hamm Christian W. , Huber Kurt , Vollert Jörn O. , Möckel Martin
Published: January 2022

Regarding the management of suspected Non-ST-segment-elevation acute coronary syndrome (ACS), the main Biomarker-in-Cardiology (BIC)-8 randomized controlled trial study had reported non-inferiority for the incidence of major adverse cardiac events at 30 days in the Copeptin group (dual marker strategy of copeptin and hs-cTnT at presentation) compared to the standard process (serial hs-cTnT testing). However, in 349 (38.7%) of the 902 patients, high-sensitivity cardiac troponin was not available for the treating physicians. High sensitivity cardiac troponin T was re-measured from thawed blood samples collected at baseline. This cohort qualified for a re-analysis of the 30-day incidence rate of MACE (death, survived cardiac death, acute myocardial infarction, re-hospitalization for acute coronary syndrome, acute unplanned percutaneous coronary intervention, coronary bypass grafting, or documented life-threatening arrhythmias), or components of the primary endpoint including death or death/MI. After re-measurement of troponin and exclusion of 9 patients with insufficient blood sample volume, 893 patients qualified for re-analysis. A total of 57 cases were detected with high sensitivity cardiac troponin T ≥ 14 ng/L who had been classified as “troponin negative” based on a conventional cardiac troponin T or I < 99th percentile upper limit of normal. Major adverse cardiac events rates after exclusion were non-inferior in the Copeptin group compared to the standard group (4.34% (95% confidence intervals 2.60–6.78%) vs. 4.27% (2.55–6.66%)). Rates were 53% lower in the per-protocol analysis (HR 0.47, 95% CI: 0.18–1.15, p = 0.09). No deaths occurred within 30 days in the discharged low risk patients of the Copeptin group. Copeptin combined with high sensitivity cardiac troponin is useful for risk stratification and allows early discharge of low-to-intermediate risk patients with suspected acute coronary syndrome is as safe as a re-testing strategy at 3 h or later.
GLI3 Is Required for OLIG2+ Progeny Production in Adult Dorsal Neural Stem Cells
Authors: Embalabala Rebecca J. , Brockman Asa A. , Jurewicz Amanda R. , Kong Jennifer A. , Ryan Kaitlyn , Guinto Cristina D. , Álvarez-Buylla Arturo , Chiang Chin , Ihrie Rebecca A.
Published: January 2022

The ventricular–subventricular zone (V-SVZ) is a postnatal germinal niche. It holds a large population of neural stem cells (NSCs) that generate neurons and oligodendrocytes for the olfactory bulb and (primarily) the corpus callosum, respectively. These NSCs are heterogeneous and generate different types of neurons depending on their location. Positional identity among NSCs is thought to be controlled in part by intrinsic pathways. However, extrinsic cell signaling through the secreted ligand Sonic hedgehog (Shh) is essential for neurogenesis in both the dorsal and ventral V-SVZ. Here we used a genetic approach to investigate the role of the transcription factors GLI2 and GLI3 in the proliferation and cell fate of dorsal and ventral V-SVZ NSCs. We find that while GLI3 is expressed in stem cell cultures from both dorsal and ventral V-SVZ, the repressor form of GLI3 is more abundant in dorsal V-SVZ. Despite this high dorsal expression and the requirement for other Shh pathway members, GLI3 loss affects the generation of ventrally-, but not dorsally-derived olfactory interneurons in vivo and does not affect trilineage differentiation in vitro. However, loss of GLI3 in the adult dorsal V-SVZ in vivo results in decreased numbers of OLIG2-expressing progeny, indicating a role in gliogenesis.
Potential of Fibrin Glue and Mesenchymal Stem Cells (MSCs) to Regenerate Nerve Injuries: A Systematic Review
Authors: Ortiz Adriana de Cássia , Fideles Simone Ortiz Moura , Pomini Karina Torres , Bellini Márcia Zilioli , Pereira Eliana de Souza Bastos Mazuqueli , Reis Carlos Henrique Bertoni , Pilon João Paulo Galletti , de Marchi Miguel Ângelo , Trazzi Beatriz Flavia de Moraes , da Silva Willian Saranholi , da Cunha Marcelo Rodrigues , Buchaim Daniela Vieira , Buchaim Rogerio Leone
Published: January 2022

Cell-based therapy is a promising treatment to favor tissue healing through less invasive strategies. Mesenchymal stem cells (MSCs) highlighted as potential candidates due to their angiogenic, anti-apoptotic and immunomodulatory properties, in addition to their ability to differentiate into several specialized cell lines. Cells can be carried through a biological delivery system, such as fibrin glue, which acts as a temporary matrix that favors cell-matrix interactions and allows local and paracrine functions of MSCs. Thus, the aim of this systematic review was to evaluate the potential of fibrin glue combined with MSCs in nerve regeneration. The bibliographic search was performed in the PubMed/MEDLINE, Web of Science and Embase databases, using the descriptors (“fibrin sealant” OR “fibrin glue”) AND “stem cells” AND “nerve regeneration”, considering articles published until 2021. To compose this review, 13 in vivo studies were selected, according to the eligibility criteria. MSCs favored axonal regeneration, remyelination of nerve fibers, as well as promoted an increase in the number of myelinated fibers, myelin sheath thickness, number of axons and expression of growth factors, with significant improvement in motor function recovery. This systematic review showed clear evidence that fibrin glue combined with MSCs has the potential to regenerate nervous system lesions.
Accumulation of Anthocyanidins Determines Leaf Color of Liquidambar Formosana as Revealed by Transcriptome Sequencing and Metabolism Analysis
Authors: Lai Jiuxin , Lin Furong , Huang Ping , Zheng Yongqi
Published: January 2022

Liquidambar formosana is important for its ornamental value in China; it is increasingly used for landscaping and gardening trees due to its diverse leaf colors and seasonal changes. Varieties including either a fixed leaf color, the purplish ‘Fuluzifeng’ (ZF), or seasonal changes in leaf color, the reddish ‘Nanlinhong’ (NLH) have been bred and registered as new plant varieties under the International Union for the Protection of New Plant Varieties (UPOV) system. To gain practical insights into the anthocyanin biosynthetic process, transcriptome sequencing (Illumina) was performed to clarify the metabolic pathways present in the three seasonal changes in leaf colors in NLH and in the springtime purple-red color of ZF. qRT-PCR was used to verify the speculation. Based on the differentially expressed genes and flavonoids analyses, the spring, summer, and autumn leaves of NLH were compared to study the seasonal differences. NLH and ZF were compared to study the formation mechanism of the two leaf colors in spring. Transcriptome sequencing produced a total of 121,216 unigenes from all samples, where 48 unigenes were differentially expressed and associated with the anthocyanidin pathway. The expression levels of LfDFR and LfANS genes corresponded to the accumulation of concentrations of cyanidins in spring (NLHC) and autumn leaves (NLHQ), respectively, with different shades of red. Moreover, the LfF3′5′H gene corresponded to the accumulation of flavonols and delphinidins in purple-red leaves (ZFC). Cyanidin and peonidin were the key pigments in red and dark-red leaves, and purple-red leaves were co-pigmented by cyanidins, pelargonidins, and delphinidins.
Perilla frutescens Leaf Extract Attenuates Vascular Dementia-Associated Memory Deficits, Neuronal Damages, and Microglial Activation
Authors: Kang Hyun-Bae , Kim Shin-Hye , Uhm Sun-Ho , Kim Do-Kyung , Lee Nam-Seob , Jeong Young-Gil , Sung Nak-Yun , Kim Dong-Sub , Han In-Jun , Yoo Young-Choon , Han Seung-Yun
Published: January 2022

Vascular dementia (VaD) is characterized by a time-dependent memory deficit and essentially combined with evidence of neuroinflammation. Thus, polyphenol-rich natural plants, which possess anti-inflammatory properties, have received much scientific attention. This study investigated whether Perilla frutescens leaf extract (PFL) exerts therapeutic efficacy against VaD. Sprague Dawley rats were divided into five groups: SO, sham-operated and vehicle treatment; OP, operated and vehicle treatment; PFL-L, operated and low-dose (30 mg/kg) PFL treatment; PFL-M, operated and medium-dose (60 mg/kg) PFL treatment; and PFL-H, operated and high-dose (90 mg/kg) PFL treatment. Two-vessel occlusion and hypovolemia (2VO/H) were employed as a surgical model of VaD, and PFL was given orally perioperatively for 23 days. The rats underwent the Y-maze, Barnes maze, and passive avoidance tests and their brains were subjected to histologic studies. The OP group showed VaD-associated memory deficits, hippocampal neuronal death, and microglial activation; however, the PFL-treated groups showed significant attenuations in all of the above parameters. Using lipopolysaccharide (LPS)-stimulated BV-2 cells, a murine microglial cell line, we measured PFL-mediated changes on the production of nitric oxide (NO), TNF-α, and IL-6, and the activities of their upstream MAP kinases (MAPKs)/NFκB/inducible NO synthase (iNOS). The LPS-induced upregulations of NO, TNF-α, and IL-6 production and MAPKs/NFκB/iNOS activities were globally and significantly reversed by 12-h pretreatment of PFL. This suggests that PFL can counteract VaD-associated structural and functional deterioration through the attenuation of neuroinflammation.
Chemical Vapor Deposition and Thermal Oxidation of Cuprous Phosphide Nanofilm
Authors: Peng Xue , Lv Yanfei , Zhao Shichao
Published: January 2022

Inorganic semiconductors usually show n-type characterization; the development of p-type inorganic semiconductor material will provide more opportunities for novel devices. In this paper, we investigated the chemical vapor deposition (CVD) of p-type cuprous phosphide (Cu3P) nanofilm and studied its thermal oxidation behavior. Cu3P film was characterized by optical microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), laser Raman spectroscopy (Raman), and fluorescence spectroscopy (PL). We found that the thickness of film ranged from 4 to 10 nm, and the film is unstable at temperatures higher than room temperature in air. We provide a way to prepare inorganic phosphide nanofilms. In addition, the possible thermal oxidation should be taken into consideration for practical application.
A Novel Method for Calcium Carbonate Deposition in Wood That Increases Carbon Dioxide Concentration and Fire Resistance
Authors: Hernandez Vicente , Romero Romina , Arias Sebastián , Contreras David
Published: January 2022

In this study, a novel method for calcium carbonate deposition in wood that increases carbon dioxide concentration and fire resistance is proposed. The method promoted the mineralization of radiata pine wood microstructure with calcium carbonate by using a process consisting in the vacuum impregnation of wood with a calcium chloride aqueous solution and the subsequent sequential diffusion of gaseous ammonium and carbon dioxide. In the most favorable conditions, the method yielded a weight gain of about 20 wt.% due to mineralization, which implied the accumulation of 0.467 mmol·g−1 of carbon dioxide in the microstructure of wood. In addition, a weight gain of about 8% was sufficient to provide fire resistance to a level similar to that achieved by a commercially available fire-retardant treatment. The feasibility of retaining carbon dioxide directly inside the wood microstructure can be advantageous for developing wood products with enhanced environmental characteristics. This method can be a potential alternative for users seeking materials that could be effective at supporting a full sustainable development.
Phase Stability and Mechanical Properties of the Monoclinic, Monoclinic-Prime and Tetragonal REMO4 (M = Ta, Nb) from First-Principles Calculations
Authors: Xiao Wenhui , Yang Ying , Pi Zhipeng , Zhang Fan
Published: January 2022

YTaO4 and the relevant modification are considered to be a promising new thermal barrier coating. In this article, phase stability and mechanical properties of the monoclinic (M), monoclinic-prime (M′), and tetragonal (T) REMO4 (M = Ta, Nb) are systematically investigated from first-principles calculations method based on density functional theory (DFT). Our calculations show that M′-RETaO4 is the thermodynamically stable phase at low temperatures, but the stable phase is a monoclinic structure for RENbO4. Moreover, the calculated relative energies between M (or M′) and T phases are inversely proportional to the ionic radius of rare earth elements. It means that the phase transformation temperature of M′→T or M→T could decrease along with the increasing ionic radius of RE3+, which is consistent with the experimental results. Besides, our calculations exhibit that adding Nb into the M′-RETaO4 phase could induce phase transformation temperature of M′→M. Elastic coefficient is attained by means of the strain-energy method. According to the Voigt–Reuss–Hill approximation method, bulk modulus, shear modulus, Young’s modulus, and Poisson’s ratio of T, M, and M’ phases are obtained. The B/G criterion proposed by Pugh theory exhibits that T, M, and M’ phases are all ductile. The hardness of REMO4 (M = Ta, Nb) phases are predicted based on semi-empirical equations, which is consistent with the experimental data. Finally, the anisotropic mechanical properties of the REMO4 materials have been analyzed. The emerging understanding provides theoretical guidance for the related materials development.
Use of Thermally Modified Jarosite for the Removal of Hexavalent Chromium by Adsorption
Authors: Picazo-Rodríguez N. , Carrillo-Pedroza F. , Soria-Aguilar Ma , Baltierra Gabriela , González Gregorio , Martinez-Luevanos A. , Almaguer Guzmán Isaías
Published: January 2022

Jarosites are residues generated during the purification of zinc and are composed mainly of iron sulfates ((Na, K)Fe3(SO4)2(OH)6). Due to the large volume of jarosite generated during the process, these residues tend to be deposited in large land areas and are not used. In the present work, jarosite was used without heat treatment (JST) as an adsorbent of hexavalent chromium contained in a sample of wastewater from a chrome plating industry under the following conditions: C0 = 200 mg/L of Cr, T = 25 °C, and pH = 3. It was only possible to remove 34% of Cr (VI). Subsequently, a thermal treatment of a jarosite sample (JTT) was carried out at 600 °C. The heat-treated sample was later used as an adsorbent in the same conditions as those for JST. The maximum chromium removal was 53%, and the adsorption capacity was 10.99 mg/g. The experimental data were fitted to the Langmuir model and to the pseudo-second-order kinetic model. It was determined that the adsorption process involved electrostatic attractions between the surface of the positively charged adsorbent and the chromium anions contained in industrial wastewater.