bluuebunny/crossref_metadata_2025 huggingface dataset:
Crypto Asset Portfolio Selection
Authors: Ahelegbey Daniel Felix , Giudici Paolo , Mojtahedi Fatemeh
Published: February 2022

The aim of this paper is to propose a portfolio selection methodology capable to take into account asset tail co-movements as additional constraints in Markowitz model. We apply the methodology to the observed time series of the 10 largest crypto assets, in terms of market capitalization, over the period 20 September 2017–31 December 2020 (1200 daily observations). The results indicate that the portfolios selected considering tail risk are more diversified and, therefore, more resilient to financial shocks.
Defining Wildfire Susceptibility Maps in Italy for Understanding Seasonal Wildfire Regimes at the National Level
Authors: Trucchia Andrea , Meschi Giorgio , Fiorucci Paolo , Gollini Andrea , Negro Dario
Published: February 2022

Wildfires constitute an extremely serious social and environmental issue in the Mediterranean region, with impacts on human lives, infrastructures and ecosystems. It is therefore important to produce susceptibility maps for wildfire management. The wildfire susceptibility is defined as a static probability of experiencing wildfire in a certain area, depending on the intrinsic characteristics of the territory. In this work, a machine learning model based on the Random Forest Classifier algorithm is employed to obtain national scale susceptibility maps for Italy at a 500 m spatial resolution. In particular, two maps are produced, one for each specific wildfire season, the winter and the summer one. Developing such analysis at the national scale allows for having a deep understanding on the wildfire regimes furnishing a tool for wildfire risk management. The selected machine learning model is capable of associating a data-set of geographic, climatic, and anthropic information to the synoptic past burned area. The model is then used to classify each pixel of the study area, producing the susceptibility map. Several stages of validation are proposed, with the analysis of ground retrieved wildfire databases and with recent wildfire events obtained through remote sensing techniques.
TiO2 Nanoparticle-Loaded Poly(NIPA-co-NMA) Fiber Web for the Adsorption and Photocatalytic Degradation of 4-Isopropylphenol
Authors: Tokuyama Hideaki , Hamaguchi Ryosuke
Published: February 2022

A TiO2 nanoparticle-loaded polymer fiber web was developed as a functional material with the ability to adsorb and photo-catalytically degrade organic pollutants in aquatic media. A linear copolymer of N-isopropylacrylamide (primary component) and N-methylol acrylamide (poly(NIPA-co-NMA)) was prepared, and composite fibers were fabricated by electrospinning a methanol suspension containing the copolymer and commercially available TiO2 nanoparticles. The crosslinking of the polymer via the formation of methylene bridges between NMA units was accomplished by heating, and the fiber morphology was analyzed by electron microscopy. 4-Isopropylphenol generated by the degradation of bisphenol A—one of the endocrine-disrupting chemicals—was used as the model organic pollutant. As poly(NIPA) is a thermosensitive polymer that undergoes hydrophilic/hydrophobic transition in water, the temperature-dependence of the adsorption and photocatalytic degradation of 4-isopropylphenol was investigated. The degradation rate was analyzed using a pseudo-first-order kinetic model to obtain the apparent reaction rate constant, kapp. The enhancement of the photocatalytic degradation rate owing to the adsorption of 4-isopropylphenol onto thermosensitive poly(NIPA)-based fibers is discussed in terms of the ratio of the kapp of the composite fiber to that of unsupported TiO2 nanoparticles. Based on the results, an eco-friendly wastewater treatment process involving periodically alternated adsorption and photocatalytic degradation is proposed.
Distinct Minor Splicing Patterns across Cancers
Authors: Levesque Lauren , Salazar Nicole , Roy Scott William
Published: February 2022

In human cells, the U12 spliceosome, also known as the minor spliceosome, is responsible for the splicing of 0.5% of introns, while the major U2 spliceosome is responsible for the other 99.5%. While many studies have been done to characterize and understand splicing dysregulation in cancer, almost all of them have focused on U2 splicing and ignored U12 splicing, despite evidence suggesting minor splicing is involved in cell cycle regulation. In this study, we analyzed RNA-seq data from The Cancer Genome Atlas for 14 different cohorts to determine differential splicing of minor introns in tumor and adjacent normal tissue. We found that in some cohorts, such as breast cancer, there was a strong skew towards minor introns showing increased splicing in the tumor; in others, such as the renal chromophobe cell carcinoma cohort, the opposite pattern was found, with minor introns being much more likely to have decreased splicing in the tumor. Further analysis of gene expression did not reveal any candidate regulatory mechanisms that could cause these different minor splicing phenotypes between cohorts. Our data suggest context-dependent roles of the minor spliceosome in tumorigenesis and provides a foundation for further investigation of minor splicing in cancer, which could then serve as a basis for novel therapeutic strategies.
Early Markers of Cardiovascular Disease Associated with Clinical Data and Autosomal Ancestry in Patients with Type 1 Diabetes: A Cross-Sectional Study in an Admixed Brazilian Population
Authors: Reis Roberta Maria Duailibe Ferreira , Azulay Rossana Santiago de Sousa , Tavares Maria da Glória , Nascimento Gilvan Cortês , Damianse Sabrina da Silva Pereira , Rocha Viviane Chaves de Carvalho , Almeida Ana Gregória , Lago Débora Cristina Ferreira , Rodrigues Vandilson , Magalhães Marcelo , Sobral Carla Souza , Parente Conceição , França Joana , Ribeiro Jacqueline , Ferraz Paulo Cézar Dias , Junior Carlos Alberto Azulay , Silva Dayse Aparecida , Gomes Marília Brito , Faria Manuel dos Santos
Published: February 2022

Patients with type 1 diabetes (T1D) have a higher risk of developing cardiovascular disease (CVD), which is a major cause of death in this population. This study investigates early markers of CVD associated with clinical data and autosomal ancestry in T1D patients from an admixed Brazilian population. A cross-sectional study was conducted with 99 T1D patients. The mean age of the study sample was 27.6 years and the mean duration of T1D was 14.4 years. The frequencies of abnormalities of the early markers of CVD were 19.6% in the ankle-brachial index (ABI), 4.1% in the coronary artery calcium score (CACS), and 5% in the carotid Doppler. A significant percentage of agreement was observed for the comparison of the frequency of abnormalities between CACS and carotid Doppler (92.2%, p = 0.041). There was no significant association between the level of autosomal ancestry proportions and early markers of CVD. The ABI was useful in the early identification of CVD in asymptomatic young patients with T1D and with a short duration of disease. Although CACS and carotid Doppler are non-invasive tests, carotid Doppler is more cost-effective, and both have limitations in screening for CVD in young patients with a short duration of T1D. We did not find a statistically significant relationship between autosomal ancestry proportions and early CVD markers in an admixed Brazilian population.
Vaccine-Induced Subacute Thyroiditis (De Quervain’s) after mRNA Vaccine against SARS-CoV-2: A Case Report and Systematic Review
Authors: Pipitone Giuseppe , Rindi Lorenzo Vittorio , Petrosillo Nicola , Foti Nunzio Adalberto Maria , Caci Grazia , Iaria Chiara , Donno Davide Roberto , Boumis Evangelo , Paviglianiti Giuseppe , Taglietti Fabrizio
Published: February 2022

De Quervain’s thyroiditis, sometimes referred to as subacute thyroiditis (SAT), is the most common granulomatous disease of the thyroid, typically found after a viral infection in middle-aged women. The mRNA encoding for the angiotensin-converting enzyme-2 (ACE-2) receptor is expressed in follicular thyroid cells, making them a potential target for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Besides infection, SARS-CoV-2 vaccines have also been implicated in SAT pathogenesis. We present a case of a woman developing SAT following vaccination with Comirnaty by Pfizer Inc. (New-York, USA). We performed a systematic review of similar cases available in the literature to provide a better understanding of the topic. We searched the databases PubMed and Embase and followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Patient records were then sorted according to the type of administered vaccine and a statistical analysis of the extracted data was performed. No statistically significant difference between mRNA vaccines and other vaccines in inducing SAT was found, nor was any found in terms of patient demographics, symptoms at presentation, initial, or follow-up blood tests. In our case report, we described the possible association between SARS-CoV-2 mRNA-based vaccine Comirnaty and SAT.
Fair Play in a Context of Physical Education and Sports Behaviours
Authors: Ludwiczak Mateusz , Bronikowska Małgorzata
Published: February 2022

The study examined prosocial behaviour and the perception of fair play in the context of physical education and sport (PES) in adolescents participating in sports, and those not undertaking sports at all. The aim of this study was to explore and indicate potential associations between an understanding of the concept of fair play and selected behaviours (e.g., abiding by the rules, obeying decisions of the referee and sportsmanship) in youth. In total, 1257 secondary school students, aged 16.1 ± 0.87 years (627 girls and 600 boys), were recruited. For assessing the level of fair play awareness (L), the Fair Play Moral Dimensions Concept Scale (FPMDCS) was used. To measure the declared level of prosocial behaviours of students, the survey My Physical Education Class (MPEC) was used. Participants were divided into groups in relation to training experience (no sports, amateur sports, professional sports) and subgroups, with levels according to their understanding of the Fair Play concept (L1 to L3). The results show significant difference in all dilemmas in various groups in relation to scores in subgroup L3 (p = 0.056; p = 0.012; p = 0.003) with regard to subgroups L2 and L1 in the level of understanding fair play. Overall, the percentage of respondents who view fair play only in the context of sport (L1) is 69%. Far fewer are those (31%) who understand it more broadly as a principle that applies to everyday life situations (L3). It seems to be crucial to promote moral development during PES activities, especially the value of respect.
Overview of Renal Replacement Therapy Use in a General Intensive Care Unit
Authors: Tiglis Mirela , Peride Ileana , Florea Iulia Alexandra , Niculae Andrei , Petcu Lucian Cristian , Neagu Tiberiu Paul , Checherita Ionel Alexandru , Grintescu Ioana Marina
Published: February 2022

Objectives. Population-based studies regarding renal replacement therapy (RRT) used in critical care populations are useful to understand the trend and impact of medical care interventions. We describe the use of RRT and associated outcomes (mortality and length of intensive care stay) in a level 1 hospital. Design. A retrospective descriptive observational study. Patients. Critically ill patients admitted to the ICU from 1 January to 31 December 2018. Interventions. Age, gender, ward of admission, primary organ dysfunction at admission, length of hospital stay (LOS), mechanical ventilation, APACHE, SOFA and ISS scores, the use of vasopressors, transfusion, RRT and the number of RRT sessions were extracted. Results. 1703 critically ill patients were divided into two groups: the RRT-group (238 patients) and the non-RRT group (1465 patients). The mean age was 63.58 ± 17.52 (SD) in the final ICU studied patients (64.72 ± 16.64 SD in the RRT-group), 60.5% being male. Patients admitted from general surgery ward needing RRT were 41.4%. The specific scores, the use of vasopressors, transfusions and mortality were higher in the RRT-group. The ICU LOS was superior in the RRT-group, regardless of the primary organ dysfunction. Conclusions. RRT was practiced in 13.9% of patients (especially after age of 61), with mortality being the outcome for 66.8% of the RRT-group patients. All analyzed data were higher in the RRT group, especially for multiple trauma and surgical patients, or patients presenting cardiac or renal dysfunctions at admission. We found significant increased ISS scores in the RRT-group, a significant association between the need of vasopressors or transfusion requirement and RRT use, and an association in the number of RRT sessions and LOS (p < 0.001).
A Systematic Scoping Review of Pre-School Self-Regulation Interventions from a Self-Determination Theory Perspective
Authors: Day Natalie , Paas Fred , Kervin Lisa , Howard Steven J.
Published: February 2022

Self-regulation (SR) is considered foundational in early life, with robust evidence demonstrating a link between early self-regulation and longer-term outcomes. This has been the impetus for a growing body of intervention research into how best to support early SR development, yet approaches and effects are diverse, which complicates an understanding of the critical characteristics for effective early SR intervention. Using Self-Determination Theory (SDT) as a guiding framework, we present a scoping review of early SR-intervention research to identify the characteristics of pre-school interventions that show significant and strong effects on young children’s SR. Studies from peer-reviewed journal articles were included if they evaluated a SR intervention with pre-school children, were published between 2010 and 2020, written in English, and included a SR outcome measure. This yielded 19 studies, each reporting the efficacy of a different SR intervention. Results showed that content factors (what interventions do) interacted with their implementation (how, when, and by whom interventions are implemented) to discriminate the more versus less efficacious interventions. Through the lens of SDT, results further suggested that targeting competence through encouragement and feedback, and nurturing children’s autonomy distinguished more from less effective interventions. Relatedness was least able to discriminate intervention efficacy.
Interrogating Patterns of Cancer Disparities by Expanding the Social Determinants of Health Framework to Include Biological Pathways of Social Experiences
Authors: Valencia Celina I. , Gachupin Francine C. , Molina Yamilé , Batai Ken
Published: February 2022

The objective of this article is to call for integrating biological pathways of social experiences in the concept model of cancer disparities and social determinants of health (SDH) fields. Black, Indigenous, and People of Color (BIPOC) populations experience more negative outcomes across the cancer continuum. Social conditions are instrumental in better understanding the contemporary and historical constructs that create these patterns of disparities. There is an equally important body of evidence that points to the ways that social conditions shape biological pathways. To date, these areas of research are, for the most part, separate. This paper calls for a bridging of these two areas of research to create new directions for the field of cancer disparities. We discuss inflammation, epigenetic changes, co-morbidities, and early onset as examples of the biological consequences of social conditions that BIPOC populations experience throughout their lifespan that may contribute to disproportionate tumorigenesis and tumor progression.
Health-Seeking Behaviors in Mozambique: A Mini-Study of Ethnonursing
Authors: Takeyama Naoko , Muzembo Basilua Andre , Jahan Yasmin , Moriyama Michiko
Published: February 2022

In settings where traditional medicine is a crucial part of the healthcare system, providing culturally competent healthcare services is vital to improving patient satisfaction and health outcomes. Therefore, this study sought to gain insight into how cultural beliefs influence health-seeking behaviors (HSBs) among Mozambicans. Participant observation and in-depth interviews (IDIs) were undertaken using the ethnonursing method to investigate beliefs and views that Mozambicans (living in Pemba City) often take into account to meet their health needs. Data were analyzed in accordance with Leininger’s ethnonursing guidelines. Twenty-seven IDIs were carried out with 12 informants from the Makonde and Makuwa tribes. The choice of health service was influenced by perceptions of health and illness through a spiritual lens, belief in supernatural forces, dissatisfaction with and dislike of the public medical system on grounds of having received poor-quality treatment, perceived poor communication skills of health professionals, and trust in the indigenous medical system. This study confirmed the need for health professionals to carefully take cultural influences into consideration when providing care for their patients. We recommend an educational intervention that emphasizes communication skills training for healthcare workers to ensure successful physician/nurse–patient relationships.
Interface Design for Products for Users with Advanced Age and Cognitive Impairment
Authors: Chen Li-Hao , Liu Yi-Chien
Published: February 2022

The aim of this study is to investigate the problems and needs of users with advanced age and cognitive impairment regarding the design and operation of daily living products. Television remote controls and an electric rice cooker were applied as the research tools, and focus group interviews with control older adults and interviews with individuals with MCI or mild dementia were conducted regarding the operation of the products. The control participants stressed that the operating procedures should not be excessively complex, the number of functions and buttons should not be overly high, and buttons and text should be enlarged. For those with MCI or mild dementia, in addition to the size and number of buttons, text size, and functions, their operation of product interfaces was affected by the complexity of the operating procedures. The solutions recommended by the participants included interface design involving direct operation and voice control.
Identification of the Visually Prominent Gait Parameters for Forensic Gait Analysis
Authors: Lee Daewook , Soon Jiman , Choi Gyuri , Kim Kijoon , Bahn Sangwoo
Published: February 2022

Walking patterns can be used as a key parameter in identifying individuals, as it varies visually depending on one’s body size as well as their habits, gender, and age group. In this study, we measure the gait characteristics of a large number of subjects using 34 visual parameters to identify significant parameters that can be used to distinguish individual walking features. We recorded 291 subjects’ walking on a constructed footpath using four video cameras, and data on parameters was calculated at the points of double support, toe-off, and heel-strike. K-means Clustering Analysis and ANOVA were conducted to determine the difference between age, gender, and BMI. As a result, we confirm that parameters related to the spine, neck, and feet are useful for identifying individuals. In the comparative analysis between age groups, the older the age, the more significant variables appeared in the upper body. The difference between genders showed significant parameters in both the upper and lower bodies of males. Similarly, among the large BMI groups, we also derived significant results in the upper and lower bodies. The key parameters derived from this study can be used more effectively in the real-world visual analysis of gait, as the walking characteristics of a large number of subjects have been measured with a similar view as real-world CCTV. This study will be effectively utilized as a foundation for future research attempting to identify people through their gait by distinguishing major gait characteristic differences.
Patellar and Achilles Tendon Thickness Differences among Athletes with Different Numbers of Meals per Day: A Cross-Sectional Study
Authors: Navarro-Ledesma Santiago , Gijon-Nogueron Gabriel , Reina-Martín Inmaculada , Ortega-Avila Ana Belen , Pruimboom Leo
Published: February 2022

The objective of this study is to analyse differences in the thickness of the patellar (PT) and Achilles tendons (AT) among athletes with different number of meals per day. The design is a cross-sectional, observational study. A total of thirty-six male athletes (with mean age groups ranging from 31 to 40) were recruited and divided into three groups based on the number of daily meals they had (3, 4 or 5 meals). PT and AT were assessed by ultrasound. There were statistically significant differences in PT when comparing groups 1 and 3, at both longitudinal (p < 0.03) and transversal (p < 0.002) planes. There were no differences when comparing groups 1 and 2 or groups 2 and 3. There was a negative correlation between the number of meals per day and tendon thicknesses in both PT (longitudinal plane: r = −0.384; p = 0.02/transversal plane: r = −0.406; p = 0.01) and AT (transversal plane: r = −0.386; p = 0.02). In conclusion, there were patellar tendon thickness differences between participants and the number of daily meals could play a key role in tendon thickness, healing and performance.
Organizational Commitment and Intention to Leave of Nurses in Portuguese Hospitals
Authors: Neves Teresa , Parreira Pedro , Rodrigues Vitor , Graveto João
Published: February 2022

Intention to leave is influenced by the commitment and individual and structural factors. It is a critical dimension in health systems due to the shortage of professionals and the potential impact on the quality of care. The present paper: (i) characterizes organizational commitment and intention to leave; (ii) analyzes the relationship between structural factors (such as, work environment and nurse staffing), individual factors (age), and nurses’ organizational commitments and intention to leave; and (iii) analyzes the differences in the intention to leave and in the organizational commitment according to service specialty, nurses’ specialization, and contractual relationship in Portuguese public hospitals. A cross-sectional study was conducted with a sample of 850 nurses from 12 public hospitals units. The results show a high affective and continuance commitment of nurses with the hospital, and a reduced tendency of the intention to leave. A significant positive association was also found between the intent to leave and individual/structural factors. Organizational commitment and intention to leave levels are satisfactory, despite the influence of several factors, such as nurse staffing, work environment, or other opportunities for professional development. The results identify particularly sensitive areas that, through adequate health and management policies, can reduce nurses’ intentions to leave and promote the sustainability of the health system.
New Insights in Prevention and Treatment of Cardiovascular Disease
Authors: Di Raimondo Domenico , Musiari Gaia , Rizzo Giuliana , Pirera Edoardo , Signorelli Salvatore Santo
Published: February 2022

Cardiovascular (CV) disease (CVD) is still a major cause of morbidity and mortality in many countries in Europe although considerable efforts have been made in recent decades to address this disease in an even more “comprehensive” approach [...]
Cadaveric and Ultrasound Validation of Percutaneous Electrolysis Approaches at the Arcade of Frohse: A Potential Treatment for Radial Tunnel Syndrome
Authors: Belón-Pérez Pedro , Calderón-Díez Laura , Sánchez-Sánchez José Luis , Robles-García Miguel , Plaza-Manzano Gustavo , Fernández-de-las-Peñas César
Published: February 2022

Entrapment of the radial nerve at the arcade of Frohse could contribute to symptoms in patients with lateral epicondylalgia or radial tunnel syndrome. Our aim was to determine the validity of applying percutaneous electrolysis, targeting the supinator muscle at the Frohse’s arcade with ultrasound imaging and in a Thiel-embalmed cadaver model (not ultrasound-guiding). Percutaneous electrolysis targeting the supinator muscle was conducted in five healthy volunteers (ultrasound study) and three Thiel-embalmed cadaver forearms. Two approaches, one with the forearm supinated and other with the forearm pronated were conducted. The needle was inserted until the tip reached the interphase of both bellies of the supinator muscle. Accurate needle penetration of the supinator muscle was observed in 100% in both US-imaging and cadaveric studies. No neurovascular bundle of the radial-nerve deep branch was pierced in any insertion. The distance from the tip of the needle to the neurovascular bundle was 15.3 ± 0.6 mm with the forearm supinated, and 11.2 ± 0.6 mm with the forearm pronated. The results of the current study support that percutaneous electrolysis can properly target the supinator muscle with either the forearm in supination or in pronation. In fact, penetration of the neurovascular bundle was not observed in any approach when percutaneous needling electrolysis was performed by an experienced clinician.
Quantification and Analysis of Micro-Level Activities Data from Children Aged 1–12 Years Old for Use in the Assessments of Exposure to Recycled Tire on Turf and Playgrounds
Authors: Lopez-Galvez Nicolas , Claude Jocelyn , Wong Patty , Bradman Asa , Hyland Carly , Castorina Rosemary , Canales Robert A. , Billheimer Dean , Torabzadeh Elmira , Leckie James O. , Beamer Paloma I.
Published: February 2022

Background: There are growing health concerns about exposure to toxicants released from recycled tire rubber, which is commonly used in synthetic turf and playground mats. To better estimate children’s exposure and risk from recycled tire rubber used in synthetic turf and playground mats, there is a need to collect detailed accurate information on mouthing activity and dermal contact behaviors. The objective of this study was to quantify and analyze micro-level activity time series (MLATS) data from children aged 1–12 years old while playing (non-sport-related games) at turf-like locations and playgrounds. Another objective was to estimate the incidental ingestion rate of rubber crumb among children. Methods: Hand and mouth contact frequency, hourly duration, and median contact duration with different objects were calculated for children playing on turf (i.e., parks, lawns, and gardens) (n = 56) and for children playing on playground structures (n = 24). Statistically significant differences between males and females as well as children’s age groups were evaluated. The daily incidental ingestion rate of rubber crumb was calculated. Results: For children playing on turf, there were significant differences between younger (1–6 y) and older (7–12 y) children for the mouthing median duration with non-dietary objects and all objects. For children playing on playground structures, we found significant mouthing frequency differences between younger (1–6 y) and older children (7–12 y) with all objects, and for mouthing median duration with non-dietary objects. There were no significant differences between males and females playing on artificial turf-like surfaces or playground mats. Our estimated mean incidental ingestion rate was 0.08, 0.07, and 0.08 g rubber crumb/day for children <2, 2–6, and 6–11 years old, respectively. Discussion: our results suggest that age and contact duration should be considered in risk assessment models to evaluate mouthing activities when children are playing on artificial turf surfaces or playground mats.
Profiles of Health-Related Patient Activation and Their Determinants: The Results of a Cluster Analysis of Older Adults—Conclusions for Patient Counselling
Authors: Włodarczyk Dorota , Chylińska Joanna
Published: February 2022

Health-related proactivity in older adults may significantly increase medication handling, adherence and patient safety. Deficiencies in training in critical characteristics and diversity of older patients may lead to medical errors in diagnosis and drug administration. This study investigated the profiles of health proactivity in older adults and the factors differentiating them, like sociodemographic factors, health status, visit characteristics, and patients’ visit-related expectations, actual experiences, and satisfaction with the visit. Before and after visits, 3391 patients aged 65–95 filled in two sets of questionnaires, that allowed to measure aforementioned factors. Three distinct proactivity profiles emerged from a cluster analysis: high (43%), medium (25%), and low proactivity (32%). Highly proactive patients had the highest expectations, but their visits provided better opportunities to meet them than in other groups. Higher proactivity was related to a longer attendance time, frequent contact with and easier access to the doctor, or a longer time spent with a patient. The findings highlight the need to detect and respond to patients’ expectations regarding psychosocial aspects of care, as well as to improve organizational aspects of care, in order to enhance health proactivity in older adults. The resulting good practice recommendations may significantly improve healthcare workers’ effectiveness in both primary and secondary care.
New Method of Avoiding Underestimation of Caries Incidence and Its Association with Possible Risk Factors in Japanese University Students: A Prospective Cohort Study
Authors: Ekuni Daisuke , Toyama Naoki , Iwasaki Yoshiaki , Morita Manabu
Published: February 2022

The objective of this three-year prospective cohort study was to investigate the association between a new definition of an increase in dental caries and risk factors in Japanese young adults. Data of Okayama University students who volunteered to undergo oral examinations and answer questionnaires in 2015 and 2018 were analyzed. The status of filled teeth and the status of occlusal/proximal surfaces of filled or decayed teeth were recorded. An increase in dental caries was defined as a change in the status of filled teeth and/or an increase in dental caries of occlusal and proximal surfaces. A total of 393 participants (18.2 ± 0.8 years) were analyzed. First and second molars showed a high prevalence of dental caries. Of the participants, 144 (36.6%) showed an increase in dental caries. In all the participants and in the females, the decayed, missing, and filled teeth (DMFT) score and history of orthodontic treatment at baseline were significantly associated with an increase in dental caries (p < 0.05) in logistic regression analyses. In the males, the DMFT score and the daily frequency of snacking (≥2) at baseline were significantly associated with an increase in dental caries (p = 0.04). The DMFT score and history of orthodontic treatment at baseline can be risk factors for an increase in dental caries using the new definition in young adults.
MYH10 Governs Adipocyte Function and Adipogenesis through Its Interaction with GLUT4
Authors: Kislev Nadav , Mor-Yossef Moldovan Lisa , Barak Rakefet , Egozi Michael , Benayahu Dafna
Published: February 2022

Adipogenesis is dependent on cytoskeletal remodeling that determines and maintains cellular shape and function. Cytoskeletal proteins contribute to the filament-based network responsible for controlling the shape of adipocytes and promoting the intracellular trafficking of cellular components. Currently, the understanding of these mechanisms and their effect on differentiation and adipocyte function remains incomplete. In this study, we identified the non-muscle myosin 10 (MYH10) as a novel regulator of adipogenesis and adipocyte function through its interaction with the insulin-dependent glucose transporter 4 (GLUT4). MYH10 depletion in preadipocytes resulted in impaired adipogenesis, with knockdown cells exhibiting an absence of morphological alteration and molecular signals. MYH10 was shown in a complex with GLUT4 in adipocytes, an interaction regulated by insulin induction. The missing adipogenic capacity of MYH10 knockdown cells was restored when the cells took up GLUT4 vesicles from neighbor wildtype cells in a co-culture system. This signaling cascade is regulated by the protein kinase C ζ (PKCζ), which interacts with MYH10 to modify the localization and interaction of both GLUT4 and MYH10 in adipocytes. Overall, our study establishes MYH10 as an essential regulator of GLUT4 translocation, affecting both adipogenesis and adipocyte function, highlighting its importance in future cytoskeleton-based studies in adipocytes.
Identification of Nifurtimox and Chrysin as Anti-Influenza Virus Agents by Clinical Transcriptome Signature Reversion
Authors: Xin Yijing , Chen Shubing , Tang Ke , Wu You , Guo Ying
Published: February 2022

The rapid development in the field of transcriptomics provides remarkable biomedical insights for drug discovery. In this study, a transcriptome signature reversal approach was conducted to identify the agents against influenza A virus (IAV) infection through dissecting gene expression changes in response to disease or compounds’ perturbations. Two compounds, nifurtimox and chrysin, were identified by a modified Kolmogorov–Smirnov test statistic based on the transcriptional signatures from 81 IAV-infected patients and the gene expression profiles of 1309 compounds. Their activities were verified in vitro with half maximal effective concentrations (EC50s) from 9.1 to 19.1 μM against H1N1 or H3N2. It also suggested that the two compounds interfered with multiple sessions in IAV infection by reversing the expression of 28 IAV informative genes. Through network-based analysis of the 28 reversed IAV informative genes, a strong synergistic effect of the two compounds was revealed, which was confirmed in vitro. By using the transcriptome signature reversion (TSR) on clinical datasets, this study provides an efficient scheme for the discovery of drugs targeting multiple host factors regarding clinical signs and symptoms, which may also confer an opportunity for decelerating drug-resistant variant emergence.
Current Studies of the Effects of Drought Stress on Root Exudates and Rhizosphere Microbiomes of Crop Plant Species
Authors: Chen Yalin , Yao Zongmu , Sun Yu , Wang Enze , Tian Chunjie , Sun Yang , Liu Juan , Sun Chunyu , Tian Lei
Published: February 2022

With the warming global climate, drought stress is considered to be the most important abiotic factor limiting plant growth and yield in the world. Drought stress has serious impacts on crop production. Many researchers have studied the influences of drought stress on crop production and plant physiology; however, few researchers have combined root exudates with root-associated microbiomes for their mutual effects under drought conditions. In this review, we systematically illustrate the impact of drought stress on root exudates and root-associated microbiomes, and then we discuss the mutual regulation of root-associated microbiomes and the host plant in helping the plant adapt to drought. Finally, we construct a framework for the mutual connections between the plant, root exudates, and the microbiome. We hope this review can provide some significant guidelines to promote the study of drought resistance in plants in association with the rhizosphere microbiota.
Diabetes and Ischemic Stroke: An Old and New Relationship an Overview of the Close Interaction between These Diseases
Authors: Maida Carlo Domenico , Daidone Mario , Pacinella Gaetano , Norrito Rosario Luca , Pinto Antonio , Tuttolomondo Antonino
Published: February 2022

Diabetes mellitus is a comprehensive expression to identify a condition of chronic hyperglycemia whose causes derive from different metabolic disorders characterized by altered insulin secretion or faulty insulin effect on its targets or often both mechanisms. Diabetes and atherosclerosis are, from the point of view of cardio- and cerebrovascular risk, two complementary diseases. Beyond shared aspects such as inflammation and oxidative stress, there are multiple molecular mechanisms by which they feed off each other: chronic hyperglycemia and advanced glycosylation end-products (AGE) promote ‘accelerated atherosclerosis’ through the induction of endothelial damage and cellular dysfunction. These diseases impact the vascular system and, therefore, the risk of developing cardio- and cerebrovascular events is now evident, but the observation of this significant correlation has its roots in past decades. Cerebrovascular complications make diabetic patients 2–6 times more susceptible to a stroke event and this risk is magnified in younger individuals and in patients with hypertension and complications in other vascular beds. In addition, when patients with diabetes and hyperglycemia experience an acute ischemic stroke, they are more likely to die or be severely disabled and less likely to benefit from the one FDA-approved therapy, intravenous tissue plasminogen activator. Experimental stroke models have revealed that chronic hyperglycemia leads to deficits in cerebrovascular structure and function that may explain some of the clinical observations. Increased edema, neovascularization, and protease expression as well as altered vascular reactivity and tone may be involved and point to potential therapeutic targets. Further study is needed to fully understand this complex disease state and the breadth of its manifestation in the cerebrovasculature.
Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects
Authors: Tian Jiahui , Dewer Youssef , Hu Haoyuan , Li Fengqi , Yang Shiyong , Luo Chen
Published: February 2022

Olfaction is a critical physiologic process for insects to interact with the environment, especially plant-emitted volatiles, during which odorant receptors (ORs) play an essential role in host recognition. Although OR gene evolution has been studied in many insect orders, a comprehensive evolutionary analysis and expression of OR gene gain and loss events among diverse hemipteran species are still required. In this study, we identified and analyzed 887 OR genes from 11 hemipteran species. The number of OR genes discovered in each species ranged from less than ten to hundreds. Phylogenetic analysis revealed that all identified Hemiptera OR genes were classified into seven major clades. Gene gain and loss events of OR have occurred in several species. Then, by positive selection, we discovered the amino acid differences between species to understand the molecular evolution of OR in the order Hemiptera. Additionally, we discussed how evolutionary analysis can aid the study of insect–plant communication. This study lays a foundation for subsequent investigations into the molecular mechanisms of Hemiptera olfactory receptors involved in host recognition.
Wolbachia in Aedes koreicus: Rare Detections and Possible Implications
Authors: Damiani Claudia , Cappelli Alessia , Comandatore Francesco , Montarsi Fabrizio , Serrao Aurelio , Michelutti Alice , Bertola Michela , Mancini Maria Vittoria , Ricci Irene , Bandi Claudio , Favia Guido
Published: February 2022

The emerging distribution of new alien mosquito species was recently described in Europe. In addition to the invasion of Aedes albopictus, several studies have focused on monitoring and controlling other invasive Aedes species, as Aedes koreicus and Aedes japonicus. Considering the increasing development of insecticide resistance in Aedes mosquitoes, new control strategies, including the use of bacterial host symbionts, are proposed. However, little is known about the bacterial communities associated with these species, thus the identification of possible candidates for Symbiotic Control is currently limited. The characterization of the natural microbiota of field-collected Ae. koreicus mosquitoes from North-East Italy through PCR screening, identified native infections of Wolbachia in this species that is also largely colonized by Asaia bacteria. Since Asaia and Wolbachia are proposed as novel tools for Symbiotic Control, our study supports their use for innovative control strategies against new invasive species. Although the presence of Asaia was previously characterized in Ae. koreicus, our study characterized this Wolbachia strain, also inferring its phylogenetic position. The co-presence of Wolbachia and Asaia may provide additional information about microbial competition in mosquito, and to select suitable phenotypes for the suppression of pathogen transmission and for the manipulation of host reproduction in Ae. koreicus.
SpAD Biofunctionalized Cellulose Acetate Scaffolds Inhibit Staphylococcus aureus Adherence in a Coordinating Function with the von Willebrand A1 Domain (vWF A1)
Authors: Pendas Stefanos , Asiminas Antonis , Katranidis Alexandros , Tsioptsias Costas , Pitou Maria , Papadopoulos Georgios , Choli-Papadopoulou Theodora
Published: February 2022

Staphylococcus aureus is one of the major pathogens causing and spreading hospital acquired infections. Since it is highly resistant to new generation antibiotics, novel strategies have to be developed such as the construction of biofunctionalized non-adherent surfaces that will prevent its tethering and subsequent spread in the hospital environment. In this frame, the domain D of protein A (SpAD) of S. aureus has been immobilized onto cellulose acetate scaffolds by using the streptavidin/biotin interaction, in order to study its interaction with the A1 domain of von Willebrand factor (vWF A1), a protein essential for hemostasis, found in human plasma. Subsequently, the biofunctionalized cellulose acetate scaffolds were incubated with S. aureus in the presence and absence of vWF A1 at different time periods and their potential to inhibit S. aureus growth was studied with scanning electron microscopy (SEM). The SpAD biofunctionalized scaffolds perceptibly ameliorated the non-adherent properties of the material, and in particular, the interaction between SpAD and vWF A1 effectively inhibited the growth of S. aureus. Thus, the exhibition of significant non-adherent properties of scaffolds addresses their potential use for covering medical equipment, implants, and stents.
A Plant-Derived Alkanol Induces Teliospore Germination in Sporisorium scitamineum
Authors: Liu Zongling , Lan Xianruan , Li Xiufang , Zhao Haiyun , Gan Jiaming , Li Ru , Chen Baoshan
Published: February 2022

Sugarcane smut caused by the basidiomycetes fungus Sporisorium scitamineum is a devastating disease for the sugarcane industry worldwide. As the initial step, the smut teliospores germinate on sugarcane buds, and subsequently, the mycelium infects the bud tissues. However, chemical signals that induce spore germination are still unknown. By comparison of the behavior of the teliospores on the buds of both resistant and susceptible varieties, we found that spore germination rates were significantly lower on the buds of resistant cultivars ZZ1, ZZ6, and ZZ9 than on the susceptible varieties GT42 and ROC22. It was found that the levels of hexacosanol and octacosanol were higher on the buds of smut-susceptible varieties than on the smut-resistant varieties. These observations were extended to the smut-resistant and smut-susceptible sub-genetic populations derived from the cross of ROC25 and YZ89-7. In artificial surface assays, we found that hexacosanol and octacosanol promoted smut teliospore germination. Transcriptome analysis of smut teliospores under the induction by octacosanol revealed that genes in the MAPK signaling pathway and fatty acid metabolism were significantly differentially expressed. Overall, our results provide evidence that alkanol plays important roles in smut teliospore germination and thus could be used as a potential marker for smut resistance in sugarcane breeding programs.
Experimental Transmission of the Yeast, Metschnikowia bicuspidata, in the Chinese Mitten Crab, Eriocheir sinensis
Authors: Jiang Hongbo , Bao Jie , Cao Gangnan , Xing Yuenan , Feng Chengcheng , Hu Qingbiao , Li Xiaodong , Chen Qijun
Published: February 2022

The Chinese mitten crab, Eriocheirsinensis, is an important farmed crustacean species in China, outranking other farmed crabs in yield and economic importance. An infection called “milky disease”, caused by the yeast, Metschnikowiabicuspidata, has emerged in E. sinensis farms in northeast China and has caused progressive economic losses. The diseased crabs present with opaque, whitish muscles and milky hemolymph. Currently, there are no effective drugs to treat the infection. Clarifying the transmission route of M. bicuspidata would help to treat and prevent the disease. We investigated the effects of three different M. bicuspidata infection methods (feeding, immersion, and cohabitation) on E. sinensis. All three infection methods led to a high infection rate in healthy crabs. After 35 d, the infection rate was 76.7%, 66.7%, and 53.3% in the feeding, immersion, and cohabitation groups, respectively. Diseased crabs exhibited the typical symptom of hemolymph emulsification, with a high pathogen load of M. bicuspidata. The yeast was not detected in the oocytes of infected crabs. Fertilized embryos, zoea larvae, and megalopae of infected ovigerous crabs tested negative for yeast, indicating that direct transmission from mother to offspring does not occur. Our results highlight avenues for the prevention and control of this yeast.
The Influence of Reactive PS-PVD Process Parameters on the Microstructure and Thermal Properties of Yb2Zr2O7 Thermal Barrier Coating
Authors: Pędrak Paweł , Góral Marek , Dychton Kamil , Drajewicz Marcin , Wierzbinska Malgorzata , Kubaszek Tadeusz
Published: February 2022

Ytterbium zirconate (Yb2Zr2O7) is one of the most promising materials for yttria-stabilized zirconia (YSZ) replacement as a thermal barrier coating (TBCs) application. In the presented report, the experimental synthesis of Yb2Zr2O7 coating using novel Reactive Plasma Spray Physical Vapor Deposition (Reactive PS-PVD) is described. The obtained coating, irrespective of the power current (1800, 2000 and 2200 A), was characterized by a hybrid structure and a thickness of about 80–110 μm. The results of XRD phase analysis showed the formation of ytterbium zirconate in the coating but the presence of ytterbium and zirconium oxides was also detected. The oxides were not observed in calcinated powder. The decrease in thermal conductivity with power current increase was observed. It was the result of higher thickness and better columnar structure of the coating obtained using higher power current of the plasma torch.
Variation of Critical Water Pressure for Hydraulic Fracturing in Cement Mortar under Sulfate Attack
Authors: Liu Detan , Zhang Hongwei , Shen Zhenzhong , Xu Liqun , Wu Qiong , Ge Yidong
Published: February 2022

Hydraulic fracturing may be induced easily in a cement-based structure in a sulfate-rich environment, which threatens engineering safety. In order to investigate the evolution of critical water pressure, a series of hydraulic fracturing tests and splitting tensile strength tests on the cement mortar under different sulfate-exposure periods are performed. The critical water pressure of the cement mortar under sulfate attack experiences an initial increase stage and a subsequent decrease stage. A stress intensity factor is modified by two proposed damage variables which are crack length and fracture stress. Then, the relationship between the critical water pressure and the tensile strength is established. Moreover, an evolution model of the critical water pressure is proposed, which reveals that the matrix tensile strength and porosity of cement mortar strongly affect the critical water pressure evolution. Additionally, an empirical formula is suggested to describe the critical water pressure evolution of the cement mortar under sulfate attack, and its validity is verified by experimental results.