bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteasome Inhibitor YSY01A Abrogates Constitutive STAT3 Signaling via Down-regulation of Gp130 and JAK2 in Human A549 Lung Cancer Cells
Authors: Huang Wei , Yuan Xia , Sun Ting , Fan Shujie , Wang Jun , Zhou Quan , Guo Wei , Ran Fuxiang , Ge Zemei , Yang Huayu , Li Runtao , Cui Jingrong
Published: August 2017

Proteasome inhibition interfering with many cell signaling pathways has been extensively explored as a therapeutic strategy for cancers. Proteasome inhibitor YSY01A is a novel agent that has shown remarkable anti-tumor effects; however, its mechanisms of action are not fully understood. Here we report that YSY01A is capable of suppressing cancer cell survival by induction of apoptosis. Paradoxically, we find that YSY01A abrogates constitutive activation of STAT3 via proteasome-independent degradation of gp130 and JAK2, but not transcriptional regulation, in human A549 non-small cell lung cancer cells. The reduction in gp130 and JAK2 can be restored by co-treatment with 3-methyladenine, an early-stage autophagy lysosome and type I/III PI3K inhibitor. YSY01A also effectively inhibits cancer cell migration and lung xenograft tumor growth with little adverse effect on animals. Thus, our findings suggest that YSY01A represents a promising candidate for further development of novel anticancer therapeutics targeting the proteasome.
Towards a Residential Air-Conditioner Usage Model for Australia
Authors: Goldsworthy Mark
Published: August 2017

Realistic models of occupant behaviour in relation to air-conditioner (a/c) use are fundamentally important for developing accurate building energy simulation tools. In Australia and elsewhere, such simulation tools are inextricably bound both in legislation and in the design of new technology, electricity infrastructure and regulatory schemes. An increasing number of studies in the literature confirm just how important occupants are in determining overall energy consumption, but obtaining the data on which to build behaviour models is a non-trivial task. Here data is presented on air-conditioner usage derived from three different types of case study analyses. These are: (i) use of aggregate energy consumption data coupled with weather, demographic and building statistics across Australia to estimate key predictors of energy use at the aggregate level; (ii) use of survey data to determine characteristic a/c switch on/off behaviours and usage frequencies; and (iii) use of detailed household level sub-circuit monitoring from 140 households to determine a/c switch on/off probabilities and their dependence on different building and occupant parameters. These case studies are used to assess the difficulties associated with translation of different forms of individual, aggregate and survey based information into a/c behaviour simulation models. Finally a method of linking the data gathering methodologies with the model development is suggested. This method would combine whole-of-house “smart”-meter data measurements with linked targeted occupant surveying.
Sounding the Nonhuman in Joyce’s “Sirens”
Authors: Tazudeen Rasheed
Published: August 2017

This essay explores Joyce’s attempt, in “Sirens”, to give articulation to the sounds made by objects and nonhuman beings, with the ultimate goal of destabilizing the boundary separating the human voice (and other forms of human expression) from nonhuman sound. The episode itself can be read as a catalogue of sounds, nonhuman and human, that interact with one another in the absence of a qualitative standard of judgment that would separate the human voice from nonhuman sound, music from “noise”, or conceptual language from sonic expression. Human characters in the episode become what Vike Martina Plock has called “soundboards”, or resonating bodies through which the sounds of their material environment achieve expression. Additionally, human bodies are fragmented metonymically into their sounding body “parts” detached from the unity of the human subject, which allows for new forms of sonorous collaboration between sounding objects and sounding body parts. Nonhuman sounds persist in contrapuntal relation with the voices and sounds of the human characters (and their sounding body parts), a phenomenon which forces us to expand our conception of the fugal form of the episode to include nonhuman entities as collaborators, or “voices”, within it. In this way, “Sirens” asks us to consider sound, and by extension music, not simply as the purely intentional product of a human consciousness, but also as a collective composition between human bodies (and body parts) and the sonic materials of their environment.
Modulatory Effect of Methanol Extract of Piper guineense in CCl4-Induced Hepatotoxicity in Male Rats
Authors: Oyinloye Babatunji , Osunsanmi Foluso , Ajiboye Basiru , Ojo Oluwafemi , Kappo Abidemi
Published: August 2017

This study seeks to investigate the possible protective role of the methanol extract of Piper guineense seeds against CCl4-induced hepatotoxicity in an animal model. Hepatotoxicity was induced by administering oral doses of CCl4 (1.2 g/kg bw) three times a week for three weeks. Group 1 (Control) and Group 2 (CCl4) were left untreated; Piper guineense (PG; 400 mg/kg bw) was administered to Group 3 (T1) by oral gavage for 14 days prior to the administration of CCl4 and simultaneously with CCl4; PG (400 mg/kg bw) was administered simultaneously with CCl4 in Group 4 (T2); and Livolin forte (20 mg/kg bw) was administered simultaneously with CCl4 in Group 5 (T3), the standard drug group. The administration of CCl4 induces histopathological alteration in the liver, with concomitant increased activities of serum hepatic marker enzymes associated with increased levels of lipid peroxidation. Similarly, there was decrease in non-enzymatic (reduced glutathione) and enzymatic antioxidants (glutathione S-transferase), superoxide dismutase, and catalase. An elevation in serum triglyceride and total cholesterol levels was noticed along with decreased levels of serum total protein. Treatment with PG 400 mg/kg bw exhibited excellent modulatory activity with respect to the different parameters studied by reversing all the above-mentioned biochemical changes significantly in the experimental animals. These results suggest that PG offered protection comparable to that of Livolin forte with better efficacy when pre-treated with 400 mg/kg bw 14 days prior to CCl4-exposure.
Longitudinal Impact of Hurricane Sandy Exposure on Mental Health Symptoms
Authors: Schwartz Rebecca M. , Gillezeau Christina N. , Liu Bian , Lieberman-Cribbin Wil , Taioli Emanuela
Published: August 2017

Hurricane Sandy hit the eastern coast of the United States in October 2012, causing billions of dollars in damage and acute physical and mental health problems. The long-term mental health consequences of the storm and their predictors have not been studied. New York City and Long Island residents completed questionnaires regarding their initial Hurricane Sandy exposure and mental health symptoms at baseline and 1 year later (N = 130). There were statistically significant decreases in anxiety scores (mean difference = −0.33, p < 0.01) and post-traumatic stress disorder (PTSD) scores (mean difference = −1.98, p = 0.001) between baseline and follow-up. Experiencing a combination of personal and property damage was positively associated with long-term PTSD symptoms (ORadj 1.2, 95% CI [1.1–1.4]) but not with anxiety or depression. Having anxiety, depression, or PTSD at baseline was a significant predictor of persistent anxiety (ORadj 2.8 95% CI [1.1–6.8], depression (ORadj 7.4 95% CI [2.3–24.1) and PTSD (ORadj 4.1 95% CI [1.1–14.6]) at follow-up. Exposure to Hurricane Sandy has an impact on PTSD symptoms that persists over time. Given the likelihood of more frequent and intense hurricanes due to climate change, future hurricane recovery efforts must consider the long-term effects of hurricane exposure on mental health, especially on PTSD, when providing appropriate assistance and treatment.
RNA Interference and BMP-2 Stimulation Allows Equine Chondrocytes Redifferentiation in 3D-Hypoxia Cell Culture Model: Application for Matrix-Induced Autologous Chondrocyte Implantation
Authors: Rakic Rodolphe , Bourdon Bastien , Hervieu Magalie , Branly Thomas , Legendre Florence , Saulnier Nathalie , Audigié Fabrice , Maddens Stéphane , Demoor Magali , Galera Philippe
Published: August 2017

As in humans, osteoarthritis (OA) causes considerable economic loss to the equine industry. New hopes for cartilage repair have emerged with the matrix-associated autologous chondrocyte implantation (MACI). Nevertheless, its limitation is due to the dedifferentiation occurring during the chondrocyte amplification phase, leading to the loss of its capacity to produce a hyaline extracellular matrix (ECM). To enhance the MACI therapy efficiency, we have developed a strategy for chondrocyte redifferentiation, and demonstrated its feasibility in the equine model. Thus, to mimic the cartilage microenvironment, the equine dedifferentiated chondrocytes were cultured in type I/III collagen sponges for 7 days under hypoxia in the presence of BMP-2. In addition, chondrocytes were transfected by siRNA targeting Col1a1 and Htra1 mRNAs, which are overexpressed during dedifferentiation and OA. To investigate the quality of the neo-synthesized ECM, specific and atypical cartilage markers were evaluated by RT-qPCR and Western blot. Our results show that the combination of 3D hypoxia cell culture, BMP-2 (Bone morphogenetic protein-2), and RNA interference, increases the chondrocytes functional indexes (Col2a1/Col1a1, Acan/Col1a1), leading to an effective chondrocyte redifferentiation. These data represent a proof of concept for this process of application, in vitro, in the equine model, and will lead to the improvement of the MACI efficiency for cartilage tissue engineering therapy in preclinical/clinical trials, both in equine and human medicine.
Daytime-Dependent Changes of Cannabinoid Receptor Type 1 and Type 2 Expression in Rat Liver
Authors: Bazwinsky-Wutschke Ivonne , Zipprich Alexander , Dehghani Faramarz
Published: August 2017

The present study was performed to investigate the diurnal expression pattern of cannabinoid receptor type 1 (CB1) and type 2 (CB2) in liver tissue of 12- and 51-week-old normoglycemic Wistar rats. By using real-time RT-PCR, daytime dependent changes in both age groups and, for both, hepatic Cnr1 and Cnr2 receptor mRNA levels were measured. Highest amount of mRNA was detected in the light period (ZT3, ZT6, and ZT9) while the lowest amount was measured in the dark period (ZT18 and ZT21). Diurnal transcript expression pattern was accompanied by comparable changes of protein level for CB1, as shown by Western blotting. The current results support the conclusion that expression pattern of cannabinoid receptors are influenced by light/dark cycle and therefore seems to be under the control of a diurnal rhythm. These findings might explain the differences in the efficacy of cannabinoid receptor agonists or antagonists. In addition, investigation of liver of streptozotocin (STZ)-treated 12- and 51-week-old rats show alterations in the diurnal profile of both receptors Cnr1 and Cnr2 compared to that of normoglycemic Wistar rats. This suggests an influence of diabetic state on diurnal expression levels of cannabinoid receptors.
Optical CAD Utilization for the Design and Testing of a LED Streetlamp
Authors: Jafrancesco David , Mercatelli Luca , Fontani Daniela , Sansoni Paola
Published: August 2017

The design and testing of LED lamps are vital steps toward broader use of LED lighting for outdoor illumination and traffic signalling. The characteristics of LED sources, in combination with the need to limit light pollution and power consumption, require a precise optical design. In particular, in every step of the process, it is important to closely compare theoretical or simulated results with measured data (obtained from a prototype). This work examines the various possibilities for using an optical CAD (Lambda Research TracePro) to design and check a LED lamp for outdoor use. This analysis includes the simulations and testing on a prototype as an example; data acquired by measurement are inserted into the same simulation software, making it easy to compare theoretical and actual results.
An Integrated Artificial Cilia Based Microfluidic Device for Micropumping and Micromixing Applications
Authors: Wu Yu-An , Panigrahi Bivas , Lu Yueh-Hsun , Chen Chia-Yuan
Published: August 2017

A multi-purpose microfluidic device that can be used for both micromixing and micropropulsion operations has always been in demand, as it would simplify the various process flows associated with the current micro-total analysis systems. In this aspect, we propose a biomimetic artificial cilia-based microfluidic device that can efficiently facilitate both mixing and propulsion sequentially at the micro-scale. A rectangular microfluidic device consists of four straight microchannels that were fabricated using the microfabrication technique. An array of artificial cilia was embedded within one of the channel’s confinement through the aforementioned technique. A series of image processing and micro-particle image velocimetry technologies were employed to elucidate the micromixing and micropropulsion phenomena. Experiment results demonstrate that, with this proposed microfluidic device, a maximum micromixing efficiency and flow rate of 0.84 and 0.089 µL/min, respectively, can be achieved. In addition to its primary application as a targeted drug delivery system, where a drug needs to be homogeneously mixed with its carrier prior to its administration into the target body, this microfluidic device can be used as a micro-total analysis system for the handling of other biological specimens.
Application of Ultra-Small Micro Grinding and Micro Milling Tools: Possibilities and Limitations
Authors: Kirsch Benjamin , Bohley Martin , Arrabiyeh Peter , Aurich Jan
Published: August 2017

Current demands for flexible, individual microstructures in high quality result in high requirements for micro tools. As the tool size defines the minimum structure size, ultra-small tools are needed. To achieve tool diameters of 50 µm and lower, we investigate the complete manufacturing chain of micro machining. From the development of the machine tools and components needed to produce and apply the micro tools, the micro tools themselves, as well as the micro machining processes. Machine tools are developed with the possibility of producing the micro geometry (cutting edge design) of micro tools as well as plating processes to produce super abrasive micro grinding tools. Applying these setups, we are able to produce ultra-small micro grinding and micro milling tools with typical diameters of 50 µm and down to 4 µm. However, the application of such tools is very challenging. The article presents possibilities and limitations in manufacturing the micro tools themselves as well as microstructures made with these tools. A special emphasis will be on the influence of the tool substrate in micro milling and grain sizes in micro grinding.
Looking Back, Looking Forward at Halogen Bonding in Drug Discovery
Authors: Mendez Lois , Henriquez Gabriela , Sirimulla Suman , Narayan Mahesh
Published: August 2017

Halogen bonding has emerged at the forefront of advances in improving ligand: receptor interactions. In particular the newfound ability of this extant non-covalent-bonding phenomena has revolutionized computational approaches to drug discovery while simultaneously reenergizing synthetic approaches to the field. Here we survey, via examples of classical applications involving halogen atoms in pharmaceutical compounds and their biological hosts, the unique advantages that halogen atoms offer as both Lewis acids and Lewis bases.
Phytochemistry, Pharmacology and Traditional Uses of Plants from the Genus Trachelospermum L.
Authors: Zhao Zefeng , He Xirui , Zhao Yuhui , Sun Ying , Chen Xufei , Cun Ye , Huang Linhong , Bai Yajun , Zheng Xiaohui
Published: August 2017

This paper is intended to review advances in the botanical, phytochemical, traditional uses and pharmacological studies of the genus Trachelospermum. Until now, 138 chemical constituents have been isolated and characterized from these plants, particularly from T. asiaticum and T. jasminoides. Among these compounds, lignans, triterpenoids, and flavonoids are the major bioactive constituents. Studies have shown that plants from the genus Trachelospermum exhibit an extensive range of pharmacological properties both in vivo and in vitro, including anti-inflammatory, analgesic, antitumor, antiviral and antibacterial activities. In Traditional Chinese Medicine (TCM) culture, drugs that include T. jasminoides stems have been used to cure rheumatism, gonarthritis, backache and pharyngitis, although there are few reports concerning the clinical use and toxicity of these plants. Further attention should be paid to gathering information about their toxicology data, quality-control measures, and the clinical value of the active compounds from genus Trachelospermum.
Consumption of Low-Calorie Sweetened Beverages Compared to Water Is Associated with Reduced Intake of Carbohydrates and Sugar, with No Adverse Relationships to Glycemic Responses: Results from the 2001–2012 National Health and Nutrition Examination Surveys
Authors: Leahy Marge , Ratliff Joseph , Riedt Claudia , Fulgoni Victor
Published: August 2017

Although the 2015 Dietary Guidelines Advisory Committee concluded that there was moderate evidence that substituting sugar-containing sweeteners with low-calorie sweeteners (LCS) reduces calorie intake and weight, dietary recommendations encourage substituting only water for sugar-sweetened beverages during weight management. This cross-sectional study evaluated the relation of water and no- and low-calorie sweetened beverage (LCSB) intake with nutrient intakes and prediabetes criteria using data from the National Health and Nutrition Examination Survey (NHANES) 2001–2012 in 25,817 adults that were free of diabetes. Although linear trends were observed with both beverages, higher LCSB intake was associated with significantly lower consumption of carbohydrates (−9.1 g/day vs. −1.4 g/day), total sugars (−10.9 g/day vs. −2.2 g/day), and added sugars (−2.0 tsp eq vs. −0.8 tsp eq) than those associated with higher water intake. Higher intake of both beverages was significantly associated with lower insulin levels (p < 0.01); however, higher intake of LCSB was also associated with lower hemoglobin A1c (HbA1c) and lower homeostatic model assessment of insulin resistance (HOMA-IR) (p < 0.01). We observed lower odds ratios for elevated HbA1c (adjusted odds ratio [OR] 0.79, 95% CI 0.64–0.98), HOMA-IR (0.68, 0.53–0.87), and insulin levels (0.63, 0.49–0.80) in LCSB among the higher (2+ servings) intake group compared to the lowest (<1 serving) intake group. Contrary to conventional wisdom, LCSB consumption was associated with equal, if not better, dietary intake and glycemic response than water consumption. Although observational in nature, these results contribute to the growing body of evidence from human studies suggesting that in addition to water, LCSBs can also be sensible choices for reducing sugars and carbohydrate intake, with no adverse associations to measures of glycemic response.
Gallic Acid-Loaded Gel Formulation Combats Skin Oxidative Stress: Development, Characterization and Ex Vivo Biological Assays
Authors: Monteiro e Silva Silas , Calixto Giovana , Cajado Juliana , De Carvalho Patrícia , Rodero Camila , Chorilli Marlus , Leonardi Gislaine
Published: August 2017

Oxidative stress, which is a result of overproduction and accumulation of free radicals, is the main cause of several skin degenerative diseases, such as aging. Polyphenols, such as gallic acid, are an important class of naturally occurring antioxidants. They have emerged as strong antioxidants that can be used as active cosmetics. The purpose of this study was to develop a gallic acid-loaded cosmetic gel formulation and characterize it using rheological, mechanical, and bioadhesive tests. Its antioxidant effect in the stratum corneum was evaluated by a non-invasive method. According to the characterization tests, the formulation exhibited skin adhesiveness and pseudoplastic behavior without thixotropy, rendering it suitable for use as a cosmetic formulation. Furthermore, the non-invasive method indicated the antioxidant effect in the stratum corneum, with the global lipid peroxide reduction being 33.97 ± 11.66%. Thus, we were able to develop a promising gallic acid-loaded gel formulation that could reduce lipid peroxides and thus combat skin oxidative stress.
A Microtopographic Feature Analysis-Based LiDAR Data Processing Approach for the Identification of Chu Tombs
Authors: Wang Shaohua , Hu Qingwu , Wang Fengzhu , Ai Mingyao , Zhong Ruofei
Published: August 2017

Most of the cultural sites hidden under dense vegetation in the mountains of China have been destroyed. In this paper, we present a microtopographic feature analysis (MFA)-based Light Detection and Ranging (LiDAR) data processing approach and an archaeological pattern-oriented point cloud segmentation (APoPCS) algorithm that we developed for the classification of archaeological objects and terrain points and the detection of archaeological remains. The archaeological features and patterns are interpreted and extracted from LiDAR point cloud data to construct an archaeological object pattern database. A microtopographic factor is calculated based on the archaeological object patterns, and this factor converts the massive point cloud data into a raster feature image. A fuzzy clustering algorithm based on the archaeological object patterns is presented for raster feature image segmentation and the detection of archaeological remains. Using the proposed approach, we investigated four typical areas with different types of Chu tombs in Central China, which had dense vegetation and high population densities. Our research results show that the proposed LiDAR data processing approach can identify archaeological remains from large-volume and massive LiDAR data, as well as in areas with dense vegetation and trees. The studies of different archaeological object patterns are important for improving the robustness of the proposed APoPCS algorithm for the extraction of archaeological remains.
Comparative Evaluation of Background Subtraction Algorithms in Remote Scene Videos Captured by MWIR Sensors
Authors: Yao Guangle , Lei Tao , Zhong Jiandan , Jiang Ping , Jia Wenwu
Published: August 2017

Background subtraction (BS) is one of the most commonly encountered tasks in video analysis and tracking systems. It distinguishes the foreground (moving objects) from the video sequences captured by static imaging sensors. Background subtraction in remote scene infrared (IR) video is important and common to lots of fields. This paper provides a Remote Scene IR Dataset captured by our designed medium-wave infrared (MWIR) sensor. Each video sequence in this dataset is identified with specific BS challenges and the pixel-wise ground truth of foreground (FG) for each frame is also provided. A series of experiments were conducted to evaluate BS algorithms on this proposed dataset. The overall performance of BS algorithms and the processor/memory requirements were compared. Proper evaluation metrics or criteria were employed to evaluate the capability of each BS algorithm to handle different kinds of BS challenges represented in this dataset. The results and conclusions in this paper provide valid references to develop new BS algorithm for remote scene IR video sequence, and some of them are not only limited to remote scene or IR video sequence but also generic for background subtraction. The Remote Scene IR dataset and the foreground masks detected by each evaluated BS algorithm are available online: https://github.com/JerryYaoGl/BSEvaluationRemoteSceneIR.
Development of a Handheld Line Information Reader and Generator for Efficient Management of Optical Communication Lines
Authors: Lee Jaeyul , Kwon Hyungwoo , Song Jaewon , Jeon Mansik , Kim Jeehyun
Published: August 2017

A handheld line information reader and a line information generator were developed for the efficient management of optical communication lines. The line information reader consists of a photo diode, trans-impedance amplifier, voltage amplifier, microcontroller unit, display panel, and communication modules. The line information generator consists of a laser diode, laser driving circuits, microcontroller unit, and communication modules. The line information reader can detect the optical radiation field of the test line by bending the optical fiber. To enhance the sensitivity of the line information reader, an additional lens was used with a focal length of 4.51 mm. Moreover, the simulation results obtained through BeamPROP® software from Synopsys, Inc. demonstrated a stronger optical radiation field of the fiber due to a longer transmission wavelength and larger bending angle of the fiber. Therefore, the developed devices can be considered as useful tools for the efficient management of optical communication lines.
Autonomous Landmark Calibration Method for Indoor Localization
Authors: Kim Jae-Hoon , Kim Byoung-Seop
Published: August 2017

Machine-generated data expansion is a global phenomenon in recent Internet services. The proliferation of mobile communication and smart devices has increased the utilization of machine-generated data significantly. One of the most promising applications of machine-generated data is the estimation of the location of smart devices. The motion sensors integrated into smart devices generate continuous data that can be used to estimate the location of pedestrians in an indoor environment. We focus on the estimation of the accurate location of smart devices by determining the landmarks appropriately for location error calibration. In the motion sensor-based location estimation, the proposed threshold control method determines valid landmarks in real time to avoid the accumulation of errors. A statistical method analyzes the acquired motion sensor data and proposes a valid landmark for every movement of the smart devices. Motion sensor data used in the testbed are collected from the actual measurements taken throughout a commercial building to demonstrate the practical usefulness of the proposed method.
Improved Spatial Differencing Scheme for 2-D DOA Estimation of Coherent Signals with Uniform Rectangular Arrays
Authors: Shi Junpeng , Hu Guoping , Sun Fenggang , Zong Binfeng , Wang Xin
Published: August 2017

This paper proposes an improved spatial differencing (ISD) scheme for two-dimensional direction of arrival (2-D DOA) estimation of coherent signals with uniform rectangular arrays (URAs). We first divide the URA into a number of row rectangular subarrays. Then, by extracting all the data information of each subarray, we only perform difference-operation on the auto-correlations, while the cross-correlations are kept unchanged. Using the reconstructed submatrices, both the forward only ISD (FO-ISD) and forward backward ISD (FB-ISD) methods are developed under the proposed scheme. Compared with the existing spatial smoothing techniques, the proposed scheme can use more data information of the sample covariance matrix and also suppress the effect of additive noise more effectively. Simulation results show that both FO-ISD and FB-ISD can improve the estimation performance largely as compared to the others, in white or colored noise conditions.
Building Extraction Based on an Optimized Stacked Sparse Autoencoder of Structure and Training Samples Using LIDAR DSM and Optical Images
Authors: Yan Yiming , Tan Zhichao , Su Nan , Zhao Chunhui
Published: August 2017

In this paper, a building extraction method is proposed based on a stacked sparse autoencoder with an optimized structure and training samples. Building extraction plays an important role in urban construction and planning. However, some negative effects will reduce the accuracy of extraction, such as exceeding resolution, bad correction and terrain influence. Data collected by multiple sensors, as light detection and ranging (LIDAR), optical sensor etc., are used to improve the extraction. Using digital surface model (DSM) obtained from LIDAR data and optical images, traditional method can improve the extraction effect to a certain extent, but there are some defects in feature extraction. Since stacked sparse autoencoder (SSAE) neural network can learn the essential characteristics of the data in depth, SSAE was employed to extract buildings from the combined DSM data and optical image. A better setting strategy of SSAE network structure is given, and an idea of setting the number and proportion of training samples for better training of SSAE was presented. The optical data and DSM were combined as input of the optimized SSAE, and after training by an optimized samples, the appropriate network structure can extract buildings with great accuracy and has good robustness.
Spatio-Temporal Variations of Health Costs Caused by Chemical Fertilizer Utilization in China from 1990 to 2012
Authors: Yang Hong , Shen Xiaoyan , Lai Li , Huang Xianjin , Zhou Yan
Published: August 2017

The health impacts caused by chemical fertilizer utilization have challenged long-term sustainable development in many countries, particularly developing countries. Based on the emergy analysis method, we estimated the temporal and spatial variations of the health costs, through atmospheric, water, and soil pathways, of chemical fertilizer utilization in China during the period from 1990 to 2012. The results showed an obvious increasing trend of health costs from 1.8 billion Yuan in 1990 to 23.0 billion Yuan in 2012, while the ratio of health costs to agriculture output value declined slowly and became stable in recent years. Regional differences were remarkable and were significantly correlated to the levels of economic development (r = 0.843 and p < 0.001) and crop-sown area in the region (r = 0.588 and p < 0.001). Economically developed regions, especially the eastern coastal provinces, had much higher costs than the western regions. Meanwhile, fertilizer consumption shifted from the eastern to the northwest region, which was the same as the health costs. This study provides a reference to estimate the health costs of fertilizer utilization, and the results highlight the importance of sustainable development in China.
Beer and Organic Labels: Do Belgian Consumers Care?
Authors: Poelmans Eline , Rousseau Sandra
Published: August 2017

We investigate whether beer drinkers are willing to pay a price premium for organic beer compared to conventional beer. Moreover, we identify subgroups of consumers with different preference patterns by investigating whether specific personal characteristics of the purchasers have an influence on this willingness-to-pay. Specifically, results are reported from a survey including a stated choice experiment of consumer decisions concerning beer purchases in Flanders (Belgium), focusing on organic labels. A non-probabilistic sampling method was used over the Internet and 334 responses were useable for the empirical analysis. Each respondent was asked to choose their preferred beer from a series of nine choice cards describing three different beer varieties. In this respect, we created a two-block design, each consisting of nine choice cards. Each respondent was randomly presented with one of the two blocks, so that an equal distribution of the blocks could be obtained. Overall, we find that our sample is statistically indifferent between a beer with an organic label and a similar beer without an organic label. This is in line with previous research that stated that consumers are unwilling to pay high price premiums for organic vice products, such as beer. We find no statistically different preferences for male or female respondents, or for members or non-members of nature protection organizations. However, we find a significant difference (p-value = 0.029) between primary beer shoppers who have a zero willingness-to-pay (WTP) for organic beer compared to similar non-organic beer and the reference group that has a negative WTP of 14 Euro per 1.5 L for organic beer. In addition, the WTP for beer drinkers older than 40 (negative WTP of 22 Euro per 1.5 L) and the WTP for frequent beer drinkers (zero WTP) are statistically different from the reference group (p-value = 0.019 and 0.000 respectively).
Enantiomeric Separation of Tramadol and Its Metabolites: Method Validation and Application to Environmental Samples
Authors: Silva Cátia , Ribeiro Cláudia , Maia Alexandra , Gonçalves Virgínia , Tiritan Maria , Afonso Carlos
Published: August 2017

The accurate assessment of racemic pharmaceuticals requires enantioselective analytical methods. This study presents the development and validation of an enantioselective liquid chromatography with a fluorescence detection method for the concomitant quantification of the enantiomers of tramadol and their metabolites, N-desmethyltramadol and O-desmethyltramadol, in wastewater samples. Optimized conditions were achieved using a Lux Cellulose-4 column 150 × 4.6 mm, 3 µm isocratic elution, and 0.1% diethylamine in hexane and ethanol (96:4, v/v) at 0.7 mL min−1. The samples were extracted using 150 mg Oasis® mixed-mode cation exchange (MCX) cartridges. The method was validated using a synthetic effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor. The method demonstrated to be selective, accurate, and linear (r2 > 0.99) over the range of 56 ng L−1 to 392 ng L−1. The detection and the quantification limits of each enantiomer were 8 ng L−1 and 28 ng L−1 for tramadol and N-desmethyltramadol, and 20 ng L−1 and 56 ng L−1 for O-desmethyltramadol. The feasibility of the method was demonstrated in a screening study in influent and effluent samples from a wastewater treatment plant. The results demonstrated the occurrence of tramadol enantiomers up to 325.1 ng L−1 and 357.9 ng L−1, in the effluent and influent samples, respectively. Both metabolites were detected in influents and effluents.
The Natural Fungal Metabolite Beauvericin Exerts Anticancer Activity In Vivo: A Pre-Clinical Pilot Study
Authors: Heilos Daniela , Rodríguez-Carrasco Yelko , Englinger Bernhard , Timelthaler Gerald , Van Schoonhoven Sushilla , Sulyok Michael , Boecker Simon , Süssmuth Roderich , Heffeter Petra , Lemmens-Gruber Rosa , Dornetshuber-Fleiss Rita , Berger Walter
Published: August 2017

Recently, in vitro anti-cancer properties of beauvericin, a fungal metabolite were shown in various cancer cell lines. In this study, we assessed the specificity of this effect by comparing beauvericin cytotoxicity in malignant versus non-malignant cells. Moreover, we tested in vivo anticancer effects of beauvericin by treating BALB/c and CB-17/SCID mice bearing murine CT-26 or human KB-3-1-grafted tumors, respectively. Tumor size and weight were measured and histological sections were evaluated by Ki-67 and H/E staining as well as TdT-mediated-dUTP-nick-end (TUNEL) labeling. Beauvericin levels were determined in various tissues and body fluids by LC-MS/MS. In addition to a more pronounced activity against malignant cells, we detected decreased tumor volumes and weights in beauvericin-treated mice compared to controls in both the allo- and the xenograft model without any adverse effects. No significant differences were detected concerning percentages of proliferating and mitotic cells in tumor sections from treated and untreated mice. However, a significant increase of necrotic areas within whole tumor sections of beauvericin-treated mice was found in both models corresponding to an enhanced number of TUNEL-positive, i.e., apoptotic, cells. Furthermore, moderate beauvericin accumulation was detected in tumor tissues. In conclusion, we suggest beauvericin as a promising novel natural compound for anticancer therapy.
Two Dimension Reduction Methods for Multi-Dimensional Dynamic Programming and Its Application in Cascade Reservoirs Operation Optimization
Authors: Jiang Zhiqiang , Qin Hui , Ji Changming , Feng Zhongkai , Zhou Jianzhong
Published: August 2017

An efficient reservoir operation technique plays a very important role in improving the water resources and energy efficiency of reservoirs. In order to effectively avoid or alleviate the “curse of dimensionality” of Multi-dimensional Dynamic Programming (MDP) in the application of cascade reservoirs operation optimization (CROO) and keep a global convergence at the same time, two dimension reduction methods are proposed in this paper. One is a hybrid algorithm of MDP and a Progressive Optimality Algorithm (POA), named MDP-POA, which combines the global convergence of MDP and the strong local search ability of POA. MDP-POA first takes the global optimal trajectory of MDP in a low discrete degree as the initial trajectory of the POA, and then implements further optimization to the obtained initial trajectory by the POA with a high discrete degree, so as to avoid the “curse of dimensionality” of MDP in high discrete degree and the dependency of the POA for the initial trajectory. The other is an improved MDP (IMDP), which first constructs a corridor by the optimal trajectory of MDP in a lower discrete degree, and then implements further optimization in the corridor by MDP with a relatively high discrete degree, so as to avoid a large number of unnecessary calculations, and shorten the run-time effectively. In a case study, the results of MDP-POA, IMDP, and MDP are compared and analyzed from the aspects of power generation and run-time. The analysis indicates that the proposed MDP-POA and IMDP both have a good application effect and are worthy of further promotion.
Comparative Study on Influences of Bank Slope Ecological Revetments on Water Quality Purification Pretreating Low-Polluted Waters
Authors: Wu Yifeng , Dai Hongliang , Wu Jianyong
Published: August 2017

To improve aquatic environmental quality and maintain channel stability against soil erosion, ecological bank slope revetments for surface water bodies were developed using a combination of prefabricated porous concrete spheres and vegetation methods, and a model set-up consisting of two equal-sized ditches with different types of bank slope revetments was constructed to evaluate the purification effects of ecological and hard revetments on water quality. The slope of one ditch was embanked with ecological revetments as an experimental treatment, while the other was embanked with hard revetments as a control. Pollutant removal from the ecological bank revetment ditch was significantly better in terms of the overall removal efficiencies of the chemical oxygen demand of manganese (CODMn), ammonia, total nitrogen (TN), and total phosphorus (TP), with two- to four-fold greater removal compared with that from hard slope revetments under the same operational conditions. Nutrient pollutants, including ammonia, TN, and TP had higher removal efficiencies than that for CODMn in both experimental ditches. The dependence of the first-order rate constant (k20) and temperature coefficient () obtained from the Arrhenius equation indicated that the removal efficiencies for ammonia, TN, and TP were higher with greater rate constants (k20) in the experimental ditch. In the ecological revetment ditch, the k20 values for CODMn, ammonia, TN, and TP were 0.054, 0.378, 0.222, and 0.266 respectively, around three-fold the values observed in the hard revetment ditch, but there was no obvious difference in values between the two ditches. The k20 values of TN and TP in both ditches showed significant positive correlations with seasonal shifts, as the removal of nutrient pollutants is highly sensitive to water temperatures.
Overexpression of LACTB, a Mitochondrial Protein That Inhibits Proliferation and Invasion in Glioma Cells
Authors: Li Hai-Tao , Dong Dao-Yong , Liu Qiang , Xu Yi-Qin , Chen Langbo
Published: August 2017

LACTB, a mitochondrial protein, was ubiquitously expressed in different mammalian tissues, such as liver, heart, and skeletal muscle. It has been shown that LACTB is downexpressed in breast cancers, and it suppresses the proliferation and promotes the apoptosis of breast cancers. However,
its role in the progression and prognosis of glioma remains unknown. In this study, we analyzed the clinicopathological features and outcomes of LACTB expression in 98 glioma patients and investigated the effects of LACTB overexpression on the proliferation, invasion, and angiogenesis of glioma
cells in vitro. We observed a significant decrease in LACTB expression in glioma, and downexpression of LACTB is correlated with a poor prognosis of glioma patients. Moreover, Cox regression analysis reveals that the LACTB is an independent prognostic indicator for glioma patients. Overexpression
of LACTB could suppress the proliferation, invasion, and angiogenesis of glioma cells. In addition, overexpression of LACTB could inhibit the expression of PCNA, MMP2, MMP9, and VEGF. Taken together, these data indicate that LACTB may serve as a promising therapeutic target for gliomas.
Long Noncoding RNA PlncRNA-1 Promotes Colorectal Cancer Cell Progression by Regulating the PI3K/Akt Signaling Pathway
Authors: Song Wei , Mei Jia-Zhuan , Zhang Mingzhi
Published: August 2017

Accumulating evidence has indicated that long noncoding RNA (lncRNA) PlncRNA-1 plays an important regulatory role in cancers. However, the expression and biological functions of PlncRNA-1 in colorectal cancer (CRC) are still unclear. In the present study, we determined the expression
of PlncRNA-1 in CRC and explored the function of PlncRNA-1 on CRC cell progression. The results showed that PlncRNA-1 was significantly increased in CRC tissues and cell lines; high PlncRNA-1 expression was associated with depth of invasion, lymph node metastasis, and TNM stage of CRC patients.
Kaplan‐Meier curve analysis showed that patients with high PlncRNA-1 expression had a poor overall survival. PlncRNA-1 knockdown remarkably reduced cell proliferation, migration, and invasion and promoted cell apoptosis in vitro. In vivo xenograft experiments showed that PlncRNA-1 inhibition
significantly suppressed tumor growth. Finally, we used an agonist (740Y-P) of the PI3K/Akt signaling pathway; function assays showed that PlncRNA-1 exerted its effects by targeting the PI3K/Akt signaling pathway in CRC. Taken together, our data suggested that PlncRNA-1 might act as an oncogene in CRC progression and serve as a potential biomarker and therapeutic target for the treatment of CRC.
Baicalein Inhibits the Proliferation of Cervical Cancer Cells Through the GSK3β-Dependent Pathway
Authors: Wu Xiaoling , Yang Zhiqin , Dang Huimin , Peng Huixia , Dai Zhijun
Published: August 2017

Baicalein, a flavonoid derived from the root of Scutellaria baicalensis, has been reported to possess multiple pharmacological activities, such as anticancer and anti-inflammatory properties. This study investigated the effect of baicalein in cervical cancer cells. Cell growth
curve and MTT assay were performed and revealed that baicalein inhibited the proliferation of SiHa and HeLa cells in a dose-dependent manner. We further found that baicalein arrested the cell cycle of SiHa and HeLa cells at the G0/G1 phase by suppressing the expression
of cyclin D1 through the downregulation of phosphorylated protein kinase B (p-AKT) and phosphorylated glycogen synthase kinase 3β (p-GSK3β) according to FACS assays and Western blotting. Moreover, when CHIR-99021, a GSK3β inhibitor, was added to baicalein-treated SiHa cells,
the expression of cyclin D1 was recovered, and cell proliferation was promoted. In conclusion, these data indicated that baicalein suspended the cell cycle at the G0/G1 phase via the downregulation of cyclin D1 through the AKT‐GSK3β signaling pathway and further inhibited the proliferation of SiHa and HeLa cervical cancer cells.
Identification and characterization of microsatellite loci in the tuliptree, Liriodendron tulipifera (Magnoliaceae)
Authors: Gutiérrez‐Ozuna Ricardo , Hamilton Matthew B.
Published: August 2017

Premise of the study:

Twenty‐three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for Liriodendron tulipifera (Magnoliaceae), a species native to eastern North America, to investigate its genetic diversity, population structure, and mating system.

Methods and Results:

Using Illumina HiSeq paired‐end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 51 amplified consistently. When genotyped using 30 to 52 total adult trees from three old‐growth populations in Maryland, Virginia, and New Jersey, USA, 23 loci were polymorphic. These loci exhibited four to 13 alleles, and observed and expected heterozygosities ranged from 0.233 to 0.865 and 0.272 to 0.876, respectively.

Conclusions:

The microsatellite marker loci presented here will be valuable in population genetic studies of L. tulipifera because they do not suffer from ascertainment bias and show high polymorphism.
Conformational impact of structural modifications in 2-fluorocyclohexanone
Authors: Martins Francisco A , Silla Josué M , Freitas Matheus P
Published: August 2017

2-Haloketones are building blocks that combine physical, chemical and biological features of materials and bioactive compounds, while organic fluorine plays a fundamental role in the design of performance organic molecules. Since these features are dependent on the three-dimensional chemical structure of a molecule, simple structural modifications can affect its conformational stability and, consequently, the corresponding physicochemical/biological property of interest. In this work, structural changes in 2-fluorocyclohexanone were theoretically studied with the aim at finding intramolecular interactions that induce the conformational equilibrium towards the axial or equatorial conformer. The interactions evaluated were hydrogen bonding, hyperconjugation, electrostatic and steric effects. While the gauche effect, originated from hyperconjugative interactions, does not appear to cause some preferences for the axial conformation of organofluorine heterocycles, more classical effects indeed rule the conformational equilibrium of the compounds. Spectroscopic parameters (NMR chemical shifts and coupling constants), which can be useful to determine the stereochemistry and the interactions operating in the series of 2-fluorocyclohexanone derivatives, were also calculated.
A Gaia-PS1-SDSS (GPS1) Proper Motion Catalog Covering 3/4 of the Sky
Authors: Tian Hai-Jun , Gupta Prashansa , Sesar Branimir , Rix Hans-Walter , Martin Nicolas F. , Liu Chao , Goldman Bertrand , Platais Imants , Kudritzki Rolf-Peter , Waters Christopher Z.
Published: August 2017

We combine Gaia DR1, PS1, Sloan Digital Sky Survey (SDSS), and 2MASS astrometry to measure proper motions for 350 million sources across three-fourths of the sky down to a magnitude of

. Using positions of galaxies from PS1, we build a common reference frame for the multi-epoch PS1, single-epoch SDSS and 2MASS data, and calibrate the data in small angular patches to this frame. As the Gaia DR1 excludes resolved galaxy images, we choose a different approach to calibrate its positions to this reference frame: we exploit the fact that the proper motions of stars in these patches are linear. By simultaneously fitting the positions of stars at different epochs of—Gaia DR1, PS1, SDSS, and 2MASS—we construct an extensive catalog of proper motions dubbed GPS1. GPS1 has a characteristic systematic error of less than 0.3

and a typical precision of 1.5–2.0

. The proper motions have been validated using galaxies, open clusters, distant giant stars, and QSOs. In comparison with other published faint proper motion catalogs, GPS1's systematic error (

) should be nearly an order of magnitude better than that of PPMXL and UCAC4 (

). Similarly, its precision (∼1.5

) is a four-fold improvement relative to PPMXL and UCAC4 (∼6.0

). For QSOs, the precision of GPS1 is found to be worse (∼2.0–3.0

), possibly due to their particular differential chromatic refraction. The GPS1 catalog will be released online and be available via the VizieR Service and VO Service.