bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of Student Attendance System based on 2.4Ghz RFID Technology
Authors: Donger Zhou
Published: May 2021

In order to improve school management of student attendance, based on the 2.4Ghz card, using RFID technology and server back-end technology, A set of student entry and exit attendance system has been designed. The current positioning method generally used distance fitting model based on RSSI. This method has very high accuracy, but the accuracy of calculation results is highly affected by the environment and the amount of data required for calculation is very large. Usually, the data need to be transmitted from the terminal to the server for processing witch increases calculation complexity. The paper proposes an algorithm based on least squares semaphore, this algorithm greatly simplifies the calculation complexity and improves the calculation efficiency.
An Res-Unet Method for Pulmonary Artery Segmentation of CT Images
Authors: Liu Zhenhong , Yuan Hongfang
Published: May 2021

In the recognition of pulmonary embolism, the accuracy of pulmonary artery segmentation plays a key role. Due to the irregular shape of pulmonary artery and the complex adjacent tissues, it is very challenging to segment pulmonary artery using traditional convolutional neural network. Therefore, an improved Res-Unet method for pulmonary artery segmentation is proposed in this paper. To begin with, the U-net structure is used as the basis structure to allow efficient information flow. Secondly, in order to improve the gradient circulation of the network, our model introduces residual connections based on the U-net structure, that is, adding a connection from the input to the output of the two convolutions and performing a convolution operation. Finally, to quick converge, we use a hybrid loss function, which is linearly combined by Dice loss and Cross Entropy loss. The experimental results show that the proposed framework ranks higher than U-net on recall, precision and Dice, yielding results comparable to that of manual segmentation.
A Novel Terrain Adaptive Landing Gear Robot
Authors: Tang Hongyan , Tian Chunxu , Zhang Dan
Published: May 2021

In order to expand the landing areas and application range of vertical take-off and landing (VTOL) aircrafts, this article proposes a new type of landing gear robot. The robot is mainly composed of a base and three limbs with planar parallel mechanisms. The control system collects depth data through a depth camera, and fuses attitude angle and velocities to establish terrain data of the landing area. According to the terrain data and the robot kinematics model, the driving variables are calculated and used to drive the robot to achieve the adaptive landing.
Binary Quantum Elite Particle Swarm Optimization Algorithm for Spectrum Allocation in Cognitive Wireless Medical Sensor Network
Authors: Zhou Jie , Qin Hu , Zhang Yao , Yang Rui , Liu Yang , Li Chaoqun
Published: May 2021

Cognitive wireless medical sensor network (CWMSN) is a new development direction of the wireless sensor network (WSN) in recent years. The requirements of CWMSN are multi-faceted such as optimized utilization of resources, energy efficiency and channel sharing. However, CWMSN also faces the problem of limited energy and spectrum. For this reason, this paper proposes a spectrum allocation model. Moreover, a new binary quantum-behaved elite particle swarm optimization algorithm (BQEPSO) based on the combination of quantum operator, elite operator and binary particle swarm optimization (BPSO) is proposed to solve the spectrum allocation problem in CWMSN. The algorithm can enable the conflict-free use of spectrum resources in CWMSN and maximize the efficiency of spectrum allocation. The BQEPSO is compared with the genetic algorithm (GA) and traditional particle swarm optimization (PSO) under the same experimental conditions. The simulation results indicate that the performance of BQEPSO is better than the other two algorithms. And BQEPSO can maintain the total throughput of the CWMSN. What’s more, BQEPSO has a high network reward and throughput.
Research on Text Multi-Feature Fusion Algorithm Based on AM-CNN
Authors: Wensong Wang , Xiange Sun
Published: May 2021

How to know the changes of students’ emotions in real time in online education has always been one of the important issues concerned by education departments and teachers. Making use of the special functions of global, part of speech and positional attention mechanisms in text processing, a deep learning network model based on the combination of multiple attention mechanisms and convolution neural networks is proposed. Firstly, the blending characteristics between the types of attention mechanism and CNN are analyzed by using the standard ChnSentiCorp_htl_all data set to clarify the effectiveness of the combination of the three attention mechanisms and CNN. Then the model is applied to the analysis of the evaluation text of the course “big data Technology principles and applications” on the MOOC of Chinese universities, and it is verified that the evaluation indexes of this model are better than the existing conventional models.
Cloud Computer Research on Table Detection Model Based on the DC-LSTM Model
Authors: Zhang Xingming , Bai Yunchao , Wei Naike , Pan Huadong , Yin Jun
Published: May 2021

In view of the fact that tables are not easy to detect, this paper designs a table detection model based on the DC-LSTM module, which references the Convolutional Long Short-Term Memory (ConvLSTM). The model uses the backbone network of target detection to extract convolution features. The feature pyramid network is used to complete the detection task, and the DC-LSTM module is embedded in a special position in the feature pyramid network. In order to evaluate the performance of the DC-LSTM module, we added the DC-LSTM module to the YOLO v3, SSD network. Specifically, we added the DC-LSTM module to the YOLO v3 network, the new model can achieve an accuracy of more than 98% on the Table Bank data set and own data set. The model in this paper can realize the automatic extraction of table document images, which is of great significance for the realization of automated data collection.
An R-SIFT Image Matching Intelligent Algorithm Applied to Hardware Trojan Detection
Authors: Sun Chen , Liang Pujiang , Li Lingling , Ma Jingjing , Jiao Licheng , Liu Fang
Published: May 2021

Malicious modification, deletion or addition of some modules in the integrated circuits (ICs) will cause the chip to be attacked, which is called Hardware Trojans (HTs). Reverse engineering (RE) is a classic destructive method and widely used in HTs detection. However, RE is very time-consuming and error prone. In this paper, based on RE, we propose the R-SIFT algorithm to match image and reformulate the Trojan detection problem as change detection problem. For the R-SIFT algorithm, the ratio of exponentially weighted averages (Roewa) operator is introduced into the scale-invariant feature transform (SIFT) algorithm. Experiments show that the proposed method has 7 to 56 times more matching points than the original SIFT can be effectively applied to HTs detection.
A Container Deployment Optimization Method for Edge Computing
Authors: Zhang Jianfeng , Liu Bin , Wang Xiaochuan
Published: May 2021

In recent years, with the rapid development of Cloud Computing, Big Data and Internet of Things, the amount of data generated by edge nodes grows profusely, which results in high network bandwidth requirements. In order to improve the QoS of Cloud Computing, edge computing has received wide-spread attention in both industry and academia. As a resource isolation technology for edge nodes, container virtualization has been widely used. However, under the condition of unstable network connections in edge environments, there are some shortcomings on native docker container, such as large amount of image data transmission, network interruption and retransmission cost, which affect the quality of edge services. Therefore, this paper proposes an Images Distribution Method based on Deduplication (IDM-Dedup), which improves the transmission efficiency of container image and reduces the time to deploy and migrate edge services. The experimental results show that IDM-Dedup is more effective than native docker container method.
Research and Practice of Steam Demand Forecasting Model in Intelligent System for Industrial Enterprises
Authors: Qian Jie , Ye Zhihui , Ding Nanzhe , Sun Zhejian , Zhang Xun
Published: May 2021

Under the environment of China’s continuous promotion of energy conservation and emission reduction, the idea of on-demand energy supply of steam power system in tobacco manufacturing industry is put forward. Through the research and optimization of prediction algorithm, we analyze the historical data and energy supply process of steam energy, and establish the on-demand energy supply prediction model of steam energy, in order to realize the on-demand energy supply prediction of steam energy in tobacco manufacturing industry.
Research on Intelligent Analysis and Prediction Model of Funds Flow Based on Non-stationary Time Series
Authors: Yue Qiang , Hu Zhongyu , Li Dongping
Published: May 2021

Funds flow prediction is critical to the stable operation of financial service institutions. In view of the non-stationary characteristics of funds inflow and outflow in financial institutions, this paper introduces the key characteristics of time series analysis, compares AR, MA, ARMA and ARIMA models, and proposes a funds flow prediction model based on ARIMA algorithm. The stationary test, pure randomness test and model order determination of time series are explained in detail. Finally, combined with the error and score of the model, the relatively optimal model is selected to predict the test data. The experimental results show that, for non-stationary time series, the model has smaller prediction error and can effectively predict funds flow.
Food-grade protease producing bacteria isolated from Indonesian soybean tempe gembus and red oncom after prolonged fermentation
Authors: Sulistyaningtyas AR , Baldivia AS , Mukaromah AH , Safitri R , Pamaya D , Lestari D , Friskayanti R , Yasin NF , Inayatul WO , Mony N , Indrajid I , Harun A , Darmawati S , Ethica SN
Published: May 2021

The search of food-grade proteases allowing broad application in food industry and protein modification is on increase worldwide due to pressure on the market of these enzymes as commodity product, both on price reduction and increasing performance. This paper reports nine protease-producing bacterial strains as new sources of food-grade protease from bacteria isolated from two Indonesian fungal fermented foods, soybean Tempe gembus and red oncom. Isolation was conducted on every other day within 5 days of storing (post fermentation) and protease production tests were then carried out on skim milk media agar. Cells of the isolated proteolytic strains were morphologically identified using SEM (Scanning Electron Microscope) and a bacterial phylogenetic tree was constructed using MEGA 7 program based on partial sequences of 16S rRNA genes of these bacteria. Based on taxa analysis and plate-based pathogenicity test,B. megateriumIROD3 and,B. amyloquefaciensIROD2,B. tequilensisISTD3, andS. carnosusIROD4 appeared to be the potential candidates of food-grade proteolytic enzyme producers. This study concluded that prolonged fungal fermentation of soybean Tempe gembus and red oncom allowed growth of known low - nonpathogenic protease producing bacteria.
Degradation performance and microencapsulation of hydrolytic bacterial consortium formulated as bioremediation agent of liquid biomedical waste
Authors: Ethica S N , Firmansyah A , Purwaningrum E , Hidayati N , Muchlissin S I , Sukeksi A , Sulistyaningtyas A R , Zilda D S , Darmawati S , Sabdono A
Published: May 2021

This study aimed to develop bioremediation agent with bacterial cells as components for the treatment of biomedical wastes from two hospitals in Semarang City (Central Java), i.e. Roemani Muhammadiyah (coded R1) and Wongsonegoro (coded R2). Single isolates and consortium of indigenous hydrolytic bacteria characterized as multiple hydrolytic enzyme producers with low-to non-pathogenic properties obtained from previous study were tested for their degradation performance. The degradation performance test is necessary to formulate components of bacterial consortium as bioremediation agent. The tests were conducted on the selected bacteria as single isolate and as consortium. The six bacteria tested as single colonies and as consortium were Bacillus velezensis R1.3, B. amyloliquefaciens R1.6, B. amyloliquefaciens R1.14, B. velezensis R1.16, B. licheniformis R2.5, and B. amyloliquefaciens R2.9. Degradation performance on biomedical waste mainly containing organic matters was assessed based on water pollution parameters on 4.0-L samples. They included a control, 6 samples treated with bacteria as single colonies, a sample treated with bacteria as indigenous consortium and a sample treated with bacteria as mixed consortium. Parameters of wastewater pollution measured included COD (Chemical Oxygen Demand), BOD (Biological Oxygen Demand), TSS (Total Soluble Solid), NH3, and PO4. Next, encapsulation of a bacterial consortium as the best condition for degradation was also carried out using maltodextrin to allow storage and preservation of the bioremediation agent for longer period. The encapsulated product was visualized in SEM images to evaluate its quality. The results showed that a consortium comprising 4 indigenous bacterial isolates from R1 hospital could decrease BOD of biomedical wastewater by 85% and TSS by 43%. Those from R2 showed performance in reducing PO4 by 21%. This study demonstrated that compared to single isolates and mixed bacterial consortium tested, the indigenous hydrolytic bacterial consortium showed better ability in improving BOD and TSS of liquid biomedical waste of R1 hospital.
Molecular systematic and phylogenetic analysis of indigenous bacterial isolates with potential as bioremediation agent based on 16S rRNA gene analysis
Authors: Iskandar A U , Ethica S N , Sukeksi A , Mukaromah A H , Sulistyaningtyas A R , Darmawati S
Published: May 2021

Liquid biomedical waste is a form of medical waste from community health centers (Pusat Kesehatan Masyarakat or Puskesmas) with high levels of health hazardous organic contaminants. Bioremediation is an alternative way to eliminate toxic components in liquid waste. A bacteria community that can be used as component of organic waste bioremediation is indigenous hydrolytic and non-pathogenic to low-pathogenic bacteria. From previous studies, 4 hydrolytic indigenous bacterial isolates with such characteristics were obtained from liquid clinical wastes of two health centers in Semarang City, namely H1, H3, H5 (from Puskesmas Halmahera), and T3 (from Puskesmas Tlogosari Kulon). This study aimed to reveal the molecular identity and kinship these bacterial isolates to understand more of their properties as consortium of bioremediation agent. Molecular identification and phylogenetic tree construction works were carried out based on 16S rRNA gene sequences. Sequences of 16S rRNA gene sequences were obtained by isolation and gene amplification using the PCR method followed by sequencing. Based on the results of molecular identification, the four isolates studied were in the same class, namely Gammaproteobacter with Phylum Proteobacter. H1 bacterial isolates have 98.01% similarity with Acinetobacter schindleri. H3 and H5 isolates share the same genus, Stenotrophomonas, with 99.79% similarity with S. maltophiphila and 97.69% with S. acidaminiphila, respectively. T3 isolate had a similarity of 98.85% with Pararheinheimera aquatica species, which was widely known as a potent bioremediation agent. The phylogenetic tree design with the MEGA 6 program showed that the H3 and H5 isolates had the closest kinship compared to the other two isolates, while the T3 isolates had the farthest relationship with the 3 other isolates. This is in line with the fact that T3 isolate was originated from a different location separating it from 3 other isolates.
Preliminary study of insecticidal effect of citronella grass essential oil (Cymbopogon nardus) against post harvest pest Sitophilus oryzae
Authors: Kardinan A , Maris P , Rizal M
Published: May 2021

Sitophilus oryzae is one of major postharvest pest in rice. It damaged the grain quantitatively and qualitatively (decreasing the product hygiene and causing the residue of its excretion). The research used natural insecticide comprise citronella grass which is a promising botanical insecticide to be used for controlling postharvest pest. This research has been conducted in the laboratory of Entomology, Indonesian Spice and Medicinal Crops Research Institute (ISMECRI) in January to March 2020. Citronella grass was distilled to obtain its essential oil containing 39% of citronella and 86% of geraniol. The research consisted of three methods, i.e. (a) repellent, designed by Completely Randomized Design, 5 treatments (concentration at 10%; 7.5%; 5%; 2.5%; and 1.25%), and 5 replications; and toxicity by using probit analysis applied by (b) contact; and (c) residue. The result revealed the oil may repel 33% at concentration of 10%, followed by concentration of 7.5%; 5% and 2.5% may repel as much as 24.6%; 20.4% and 10.8% consecutively. The LC50 of contact and residue toxicity are 23.2% and 1.71%. Applied by residual is more toxic than by contact. This showed that citronella grass essential oil has good prospect as natural insecticide to control S. oryzae, therefore the research need to be elaborated.
Stimulation effect of synthetic plant growth regulator (GA3 and BAP) on young cinchona plant (Cinchona ledgeriana) grown in lowland
Authors: Maxiselly Y , Anjasari I R D , Sutari W , Ariyanti M , Soleh M A , Sari R A , Chiarawipa R
Published: May 2021

Cinchona is a pharmaceutical plant that produces medical substance. This plant contains copious type of alkaloid such as quinine, quinidine, cinchonine, and cinchonidine. Several obstacles might restrict cinchona cultivation, mainly climatic factors such as temperature and rainfall. One of the efforts to improve cinchona growth in lowland through the application of synthetic plant growth regulator (PGR). Two stages of experimental design were used in this study: first stage was the application of GA3 in six levels from October to December 2017 and the second stage was six levels of BAP concentrations treatment from July until October 2018. The experimental design used was randomized block design (RBD) with 4 replications at low altitude area for cinchona plant. The results showed GA3 applications have a significant effect towards leaves width and plant height. The 225 ppm of GA3 dosage produced maximum leaf width, 100ppm yielded the maximum number in plant height, and 150ppm produced the highest leaf count. While GA3 as a PGR improved the vegetative characters, various concentration of BAP had positive impact for stem diameter, leaves number, leaves area, and chlorophyll content on young cinchona plant grown in lowland area.
Climate based model in determining the distribution pattern of Cecropia peltata L across global landscape
Authors: Yudaputra A , Hutabarat P
Published: May 2021

Climate change becomes a major threat to the global biodiversity. It alters the ecological niche of species, even small change in temperature could have a significant impact to the distribution pattern of biodiversity. Cecropia peltata is an invasive species with wide range geographic distribution. The aim of this study is to understand the impact of climate change to the current and future distribution of invasive plant C. peltata. The Support Vector Machine (SVM) and Boosted Regression Tress (BRT) algorithm of machine learning were used to predict the current and future distribution. The occurrence records of C. peltata was obtained from Global Biodiversity Information Facility (GBIF). There were 2691 occurrence records in GBIF database. The global climatic variables with the resolution 2.5 km were used as predictors of model. VIF was used to select the multicollinearity among those variables using threshold of 0.7. The CIMP5 of Global Circulation Model (GCM) was used to understand the impact of climate change to the distribution of the plant. The future projection on year 2070 with the worst climate scenario RCP 8.5 was used on these predictive models. The SVM and BRT models were actually relevant to be used as predictive models with AUC >0.90 and categorised as excellent predictive models. The future distribution pattern was likely to be shifted compared to the current distribution prediction. The output of this study as predictive current and future distribution maps would be useful to provide an information about the potential area where the species might be invading based on the training data (observation data). Furthermore, the prediction of future distribution would be necessary to understand how the climate change literally affects the range of distribution of the invasive plant species.
Ethnobotanical knowledge on jamu herbal drink among consumer in Semarang
Authors: Husain F , Yuniati E , Arsi A A , Wicaksono H , Wahidah B F
Published: May 2021

Jamu is an herbal drink made from various types of medicinal plants. Jamu is very popular in Indonesia, especially for Javanese people. This herbal drink is considered a natural healthy drink to treat diseases and keep the body healthy if consumed continuously. This study explores about knowledge of jamu and medicinal plants consumed daily by consumers of the Jamu Ngadirgo in Semarang. In this study, the authors used qualitative research methods. Data were collected by using observation and interview technique. There were 21 informants who are also consumers of Jamu Ngadirgo who participated in this research. In this study, there were eight types of jamu that are commonly consumed daily by consumers. The types of jamu Ngadirgo Semarang are cabe puyang, kunir asem, beras kencur, temulawak, pahitan, surih/sirih, kunir kentel, and wejahan. There are 13 species of medicinal plants from 8 families used as ingredients to make jamu, as well as two non-plant ingredients that were mixed. However, the consumers could only mention 8 of 13 species of medicinal plants commonly used in herbal medicine. They are cabai jawa, kencur, kunyit, temulawak, asam jawa, brotowali, sambiroto, and sirih. Some parts of plants as medicinal ingredients consist of roots, leaves and rhizomes. Non-plant ingredients that are mixed in making herbal medicine are palm sugar and salt.
Katilayu (Lepisanthes rubiginosa (Roxb.) Leenh.) Population In Mt. Baung Nature Tourism Park
Authors: Mudiana Deden , Ariyanti E.E.
Published: May 2021

Katilayu (Lepisanthes rubiginosa) is one of the native plants of Indonesia that has not been much studied for the information on its existence and its potential utilization. This species has the risk of decreasing its population in nature due to several things, including the assumption of some people that this plant produces “fossil latex” which has magical abilities that increase the potential for exploitation; the other potential benefits and uses are not well known, so their cultivation is neglected; and the decreasing extent of forests in Indonesia. Research at Mt. Baung Nature Tourism Park (NTP), Pasuruan, East Java was carried out to determine the population of katilayu in the conservation area, to be precise by analyzing the vegetation and data collection on the number of individuals in the conservation area of Mt. Baung NTP. Observation of the physical environment where it grew was also done to find out the picture of the condition of the environment in which it grows. From this study it was known that the condition of the population in Mt. Baung was still quite good with the composition of individual densities at each growth stratum still showing a normal graph, that was inverted J-shaped form. Its presence was relatively scattered throughout the study site.
Evaluation of mungbean lines for resistance to Cercospora leaves spot and powdery mildew (Erysiphe polygoni) diseases in the green house
Authors: Sumartini  , Trustinah
Published: May 2021

Mungbean is an important food crop, it is a source of carbohydrates, protein and B vitamine. Therefore the production must be increased. In increasing the production of mungbean, there are several constrains including disease infection. Leaf spot and powdery mildew are the main diseases on mungbean. Planting the resistant varieties to leaf spot / powdery mildew is an effective, easy, and inexpensive way. The aim of research is to get some resistant mungbean lines against leaf spot and powdery mildew disease. The research was conducted in the greenhouse of the Indonesian Legumes and Tuber Crops Research Institute, from May to September 2019. Evaluation of the resistance of mungbean lines to leaf spot and powdery mildew was carried out consecutively. The material tested was 20 mungbean breeding lines from the breeding section, with one resistant variety and one susceptible variety as a check. Inoculation was done by spraying the suspension of Cercospora cruenta spores of leaf spot, or Erysiphe polygoni spores of powdery mildew, with a density of 104 spores / ml in the afternoon. Observation of the intensity of leaf spot or powdery mildew disease is carried out three times, starting at one week after the first symptoms appeared. The results showed that out of 20 mungbean lines tested, there were no line that were resistant, 7 lines were moderately resistant and 13 lines were moderately susceptible to leaf spot disease. Out of 20 lines of mungbeans tested, 8 lines were resistant and 12 lines were moderately resistant to powdery mildew.
Evaluation of total phenolic, flavonoid contents, antioxidant and cytotoxicity activities of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit
Authors: Christina Y I , Nafisah W , Widodo  , Rifa’i M , Djati M S
Published: May 2021

The objective of this study was to evaluate the total phenolic and flavonoid contents and to investigate antioxidant and cytotoxic activities of ethanol extract of pericarp, mesocarp and seeds of Phaleria macrocarpa (Scheff.) Boerl. The total phenolic content was determined as Gallic acid (GAE) equivalent, and flavonoid contents were determined as Quercetin (QE) equivalent. 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to measure the antioxidant activity. The cytotoxicity activities were tested against breast cancer cell lines T47D using WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1,3-benzene sulfonate) assay. The ethanol extract of pericarps, rich in flavonoid (43.88 ± 2.33), exhibited the most potent antioxidant activities to DPPH (IC50: 16.01 μg/mL). However, a high amount of phenolic content (66.57 ± 2.33 mg GAE/g) was found in the ethanol extract of P. macrocarpa mesocarp. The ethanol extract of P. macrocarpa seeds exhibited high cytotoxic activities against T47D cell lines in a dose-dependent manner compared to mesocarp and pericarp extracts. These results suggested that the ethanol extract of P. macrocarpa seeds could be used as an anticancer against T47D cell lines.
Organic farming system of cocoa plantations in South Malang, Indonesia
Authors: Leksono A S , Mustafa I , Gama Z P , Afandhi A , Zairina A
Published: May 2021

Malang Regency is one of the cocoa producers in East Java, Indonesia with an area of more than 3,000 hectares. Some cocoa plantation in Malang have implemented organic farming systems. The purpose of this study was to reveal the organic farming process and system of cocoa plantations in South Malang. This research was conducted in Sumbermanjing Wetan Sub-district from May to July 2020 with a survey method of 40 respondents, selected by snowball sampling. The results showed that 97.5% of farmers understood about organic farming systems. Most of their knowledge comes from field instructors and fellow members of farmer groups. The practice of organic farming that they do is by using organic fertilizer derived from manure, compost, and liquid organic fertilizers. Furthermore, farmers also use 16 plant species as botanical pesticides. In pest control, 65% of farmers also understand the importance of natural enemies. One of the most popular natural enemies is the weaver ants (Oecophylla smaragdina) or as a pest control Tea mosquito bug (Helopeltis antoniii). Type of effort to preserve: preparing special trees, making nests, leaving them alive and breeding, and avoiding sprayed with pesticides. However, farmers still undergo obstacles such as field maintenance and processing, less effective pest control, expensive of market bio-pesticides price products, declining crop yields, and marketing difficulties.
Combining ability and heterosis to root-knot nematode resistance on seven genotypes of kenaf using full diallel cross analysis
Authors: Parnidi  , Soetopo L , Damanhuri  , Marjani
Published: May 2021

A full diallel cross is confirmed to be able to advocate kenaf breeders to select breeding materials, such as inbred lines that produce the best combination with heterosis properties. The results of this study provided information about general combining abilities (GCA) of parents, specific combining ability (SCA), as well as heterosis values. This study aims to estimate the combining abilities and heterosis values of root-knot nematodes in kenaf originated from the full diallel cross. Semi-field research was conducted in an experimental garden located at Karangploso Balittas, from November 2018 to January 2019. The variance of GCA values was higher than SCA values for the number of root knot, reproductive factors, and number of egg mass. In contrast, the variance of GCA values was lower than SCA values for the number of second-stage juvenile (J2) and the number of egg per egg mass characters. KR4, K45, and KR15 genotypes indicated negative GCA values on all characters tested. Meanwhile, KR1, Kin2 and DS028 genotypes signified susceptibility with a positive GCA value. The crossbreeding combinations showed negative SCA values on the number of second-stage juvenile, the number of egg mass and the number of egg per egg mass characters of resistance to root-knot nematodes (M. incognita), i.e. KR6 x DS028. KR4, KR5 and KR15 genotipe was quite consistent with negative GCA and SCA values, as well as heterosis values for characters of resistance to root-knot nematodes on kenaf roots on the number of second-stage juvenile (J2), the number of egg mass and the number of egg per egg mass characters tested. Genotype of KR4 x KR5 and its reciprocal have negative heterobeltiosis values on number of second-stage juvenile, egg mass, and the number of eggs per egg mass evaluated characters
Morphological characters review on white-lipped frog (Chalcorana chalconota; Schelgel, 1837) based on morphometrical analysis, within the population of Java
Authors: Priambodo B , Hamidy A , Kurniawan N
Published: May 2021

Chalcorana chalconota or White-Lipped Frogs are widely distributed in Indonesia, Malaysia, and Thailand. Described as a species complex, C. chalconota from Java Island is very necessary to be studied its taxonomic status. Intra-population studies are important to determine the representation of a population in a particular place. Therefore, we have aimed to elaborate on the relationship of C. chalconota within the Java population using a morphometric approach. This research was conducted in the Laboratory of Herpetology, Research Center for Biology, Indonesian Institute of Sciences. Morphometric measurements were performed totally on 47 individuals of C. chalconota from Java Island with 29 morphological characters. Data analyses using Principal Component Analysis (PCA) and cluster analysis with Bray-Curtis Index. PCA on both sexes investigated a random grouping, both samples from West Java, Central Java, East Java, and Banten. Clustering analyses on male and female population are indicating high indexed of Bray Curtis with score up to 0.9, means very closely related among individual. Such as PCA, clustering analysis produces the same grouping patterns (random) from different localities. Based on PCA and clustering analysis, we interpreted that C. chalconota in the Javanese population was closely related with evidence of conserved morphological characters.
Comparison of serum protein profile in Indonesian Local Ettawah goats with single and twin offspring using SDS-PAGE
Authors: Mudawamah M , Putri GR , Sumartono  , Ciptadi G
Published: May 2021

Indonesian Local Ettawah Goats (ILEG) is local Indonesian livestock with more than one offspring potential. There is no description of the serum protein profile of female goat with single and twin offspring. This study aimed to compare the protein band type, the percentage of protein band appearance, protein band thickness between the female goat serum of single and twin offspring. This research method was a case study at the breeding village of Ampel Gading Malang East Java, Indonesia. The sample came from ILEG female with single and twin offspring, which had a record of three offsprings with six replications per group. Serum samples were isolated from whole blood taken through the goat jugular vein. Separation of blood serum with Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and performed by Panther Data Base for analysis of protein type data. The results showed that single and twin ILEG had ten types of protein bands 13-140 kDa with an average percentage of protein band appearances of 8.32% higher in twin offsprings compared to single offspring. The thickening level of a protein band at 44-94 kDa in female goat with twin offsprings was increased expression compared to single offspring. The ILEG protein profile of twin offspring was higher in quality and quantity than single offspring. The research recommends molecular protein weight at 44-94 kDa as a candidate to an early detect female goat with twin offspring.
Resistance of ten local probolinggo tobacco accessions to armyworm (Spodoptera litura F.) in field
Authors: Prabowo H , Adikadarsih S , Damaiyani J
Published: May 2021

Tobacco (Nicotiana tabaccumL.) is an important cash crop of Indonesia. This commodity is great economic importance and a source of revenue, employment, and foreign exchange. Tobacco production in Indonesia faces several obstacles, one of which is damage caused by armyworm (Spodoptera litura) infestation. The armyworm cause destructive effects on plant so that it is necessary to obtain information regarding the types of tobacco, especially new tobacco varieties which are resistant to armyworm. Therefore, the production of tobacco could be improved. This research aimed to identify the resistance of local Probolinggo tobacco to armyworm so that they can be utilized to develop armyworm resistant tobacco and to improve productivity. A study to test the field resistance of local Probolinggo Tobacco was conducted in Besuk and Krejengan Village, Probolinggo. Eight accessions with two controlling accessions (Paiton 1 and 2) were used. The results showed that the average percentage of armyworm infestation on Vis-à-vis tobacco accession was approximately 9.0 up to 17.33 %. The most attracting accessions are DB Srongsong Sadhana, DB Srongsong Gajah, and Paiton 1 with the percentage of infestation of 19.50; 14.34 and 14.17 %, respectively. The lowest armyworm infestation was found in two tobacco accessions i.e. Samporis and Jimamwut. The level of resistance based on Chiang and Talekar showed that Samporis and Jimamwut were moderately resistant to armyworm, but accession DB Serongsong Sadhana dan Paiton 1 were susceptible. Samporis and Jimamwut can be developed as candidates to face army worm infestation.
The comparison of morphological characters and capsaicinoid contents of the 4th generation chili pepper genotypes G1/01 and G7/01 (Capsicum frutescens L.)
Authors: Tabroni I , Siswanto D , Arumingtyas EL
Published: May 2021

The study aims to identify the morphological and physiological variation of the 4th generation of C. frutescens L. G1/01 and G7/01. The morphological data measured based on IPGRI, AVRDC and CATIE descriptors, and capsaicinoid content measured using spectrophotometry were used to build Jaccard similarity-based cladogram. The results showed that 40% of the G1/01 and 100% of the G7/01 plant height were categorized into very high category (> 85 cm). One hundred percent of the G1/01 and G7/01 were categorized into very early and late flowering, respectively. Eighty-five percent of the G1/01 and 100% of the G7/01 fruit length were categorized into normal (1-5cm). Ninety percent of the G1/01 and 56% of the G7/01 fruit diameters were categorized into normal (>0.5-1cm). Eighty-nine percent of G1/01 and 100% of G7/01 fruit weight were categorized into moderate (0.1-2g). Eighty percent of the G1/01 and 100% of the G7/01 number of seeds were categorized into moderate (>50-200 seeds) and small (<50 seeds), respectively. The capsaicinoid content of G1/01 and G7/01 was significantly lower than the original type. Mutant T39 (G7/01) had the highest capsaicinoid content (19.35mg/g). In the dendrogram, genotypes G1/01 and G7/01 were separated based on the character of the node color, petal color, peel surface, leaf color, and flowering time. G1/01 formed five clusters while G7/01 formed two clusters. G1/01 is more varied than G7/01.
The effect of the container for tpm (top of paper method) test on germination and infection rate of porang (Amorphophallus muelleri) seeds
Authors: Harijati N , Fikri HM
Published: May 2021

Now, the need for porang seedlings is very urgent, so it is necessary to provide seedlings in a short amount of time. Among the sources for propagation of porang, the use of seeds as planting material promises to supply large quantities of seedlings. The initial step of preparing the seedlings is germination. The success of germination determines the next step. The purpose of this study was to examine the effectiveness of using trays and jars in reducing infection rates and to determine the rate of germination of porang seeds. This study used a complete random design with 3 replications. Each replication consisted of 50 seeds. While data analysis used unpaired t-test. And the germination method used the top-on-paper method (TPM). The observed-parameters included germination rate at 7 days after planting (dap), 17 dap, and 30 dap; percentage of seed contamination at 7 dap and 11 dap; the percentage of the number of sprouts rooted at 28 dap and 42 dap. The results showed that the percentage of germination using trays and jars at 7 dap, 17 dap, or 30 dap were not significantly different. Seed contamination at 7 dap and rooted-sprouts at 28 dap were not significantly different between tray and jar. However, for seed contamination at 11 dap, the tray produced higher contamination than the jar and the results were significantly different. As for the rooted sprouts at 42 dap, the trays produced lower rooted sprouts than the jar, 44.67% and 89.33% respectively and significantly different. The conclusion of this research was that germination on top-paper method using a jar was better than a tray
The consequence of increasing tree diversity was reducing tree basal area at across different management of agroforestry system of Bangsri Wathershed
Authors: Prayogo C , Waskitho AH , Muthahar C
Published: May 2021

The Planting more trees in terrestrial ecosystem had provided on the increasing on many ecosystem services including carbon storage, water supply and soil cover. The relationship between the impact on increasing tree density on tree diversity across various land use type are limited. Using a survey and collecting field measurement within the plot in the size of (100 m × 20 m), we investigated the effect of different type land use on tree diversity, basal area, and species composition at Bangsri Watershed-East Java, comparing different agroforestry system to various young to old production forest of Mahogany (Sweitenia mahogany), Pines (Pinus merkusii) and Rekisi (Magnolia × alba (D.C.). The result showed that seedling population under complex agroforestry system was almost twice (389 individuha-1) higher that those simple agroforestry system or production forest. Young Mahogany (4-5 years) provide the highest number of poles, whilst old Mahogany and Pines production forest (30-40 years) were contributed the greatest tree population at (500 – 600) individuha-1 providing the highest value of basal area since the average of those tree diameter within the size of (25-40 cm). Tree population of complex agroforestry system were lower 50% than production forest. However, diversity indices (H’) of sapling, pole and tree under complex agroforestry system were between 1 to 2.5, while the others plot were<1, except for seedling diversity. In particular the increasing of tree population impact of greater basal area but however it reduced the diversity of, seedling, sapling, pole and tree as the consequences of the implementation of different tree and land management.
Changes in the morphology of catfish infected by Edwardsiella tarda as indicator of food safety
Authors: Kartikaningsih H , Yahya  , Setijawati D , Ma’rifat T N , Pramesti WS
Published: May 2021

Edwardsiella tarda is a pathogenic bacterium in catfish. The aim of the research was to observe the morphological changes of catfish infected by Edwardisella tarda as catfish safe consumption indicator. Catfish was artificially infected with Edwardsiella tarda in aquarium for 7 days. The observation included catfish behavior, organoleptic freshness, meat morphology and the number of Edwardsiella tarda found in catfish skin, meat as well as in aquarium water. At the end of culture, it was known that the number of Edwardsiella tarda in meat, skin and water were 105 cfu/g, 108cfu/g and 102 cfu/mL. The fishy smell in aquarium could be sensed starting from day 3 after the pathogen introduction which was in concomitant with appearing red spots in the catfish skin. The freshness of catfish based on organoleptic tests was still around 9-7. From the SEM photographs showed the change in the structure of catfish meat. Histology observation showed the myofibril fragility increased. It recommended for consumers to keep on eye in appearance of red the spots color on catfish skin since it was visible indicator of early stage of spoilage caused by pathogen.
Monitoring quality of water sources in Madura: physicochemical and biological
Authors: Vidayanti V , Retnaningdyah C , Arisoesilaningsih E
Published: May 2021

This study aims to (1) investigate the water sources quality based on physicochemical and biological parameters and (2) compare the water sources quality among locations of the study. The water sample takes from four locations water sources in Madura: Bangkalan area, Jokotole (JT), and Sumber Pocong (SP); Pamekasan, Samiran Reservoir (SR); and Sumenep, Nyapar (NY). Three sampling stations per location with three replications (station) are selected for sample collection. Some physicochemical parameters are measured, and phytoplankton samples are collected vertically and identified using keys from some references. The physicochemical parameters show that three locations are suitable for natural water sources based on the DO and salinity level: SP, SR, and NY. JT has the richest taxa than the other locations, but the highest number of plankton belongs to SP. The Importance Value Index (IVI) of plankton shows no dominance of plankton species in every water supply source location and is supported by the Simpson dominance index result. The evenness index shows a high evenness level of the species in every site. Based on the results of Shannon Wiener diversity index of plankton, the level of toxicity in the four research locations is in the light to moderate level.
Orchids diversity at RPH Ngebel, Ponorogo, East Java
Authors: Yulia N D , Ariyanti E E
Published: May 2021

The research on the diversity of orchids at the Ngebel - Ponorogo RPH area, precisely in the Ringin Putih and Gunung Picis Forest Reserve was done. The results showed that there were 20 orchid species were consisting of 15 epiphytic orchids that belonged to 10 genera and 5 terrestrial orchids that belonged to 5 genera. Epiphytic orchids found in the area grow on host trees such as Coffea trees, Ficus and Litsea.
The amplification and analysis of cytochrome oxidase 1 (CO1) Gene of Ornithoptera croesus from Bacan Island
Authors: Mas’ud Abdu , Roini C , Lahati B.K. , Sundari
Published: May 2021

Ornithoptera croesus is one of the endemic butterflies in Bacan island. The results of previous studies stated that O croesus has genetic variation based on morphological and molecular character of RAPD. In male and female butterflies there are variations in color and body size. The purpose of this study was to amplify the CO1 gene in Ornithoptera croesus butterflies collected from Mount Sibela in Bacan Island. The method used in this study is total DNA isolated from tissue using the ZymoBiomic (Zymo Research DNA Extraction) Kit, DNA amplification using the CO1 gene, sequencing and BLASTn analysis. The results showed that the specimen was successfully amplified with an amplicon size of 500 bp. Furthermore, the results of the BLASTn analysis revealed that the sequence had a 99% similarity with O croesus NCBI in both males and females, and based on phylogenetic analysis it had a closed position. The application of CO1 DNA barcodes is effective for identifying butterflies at species level.
Determining strategic decisions for local business sustainability in broiler partnerships
Authors: Andriani D P , A’yunin Q , Utami W R , Nelson R
Published: May 2021

The broiler is one of the diversity of chicken species that has an upward trend in the public’s amount of consumption every year. Market uncertainty caused by government policies affects the level of production and the price of the broiler. This is detrimental to the poultry farmers and the partnership industry because the selling price becomes lower than the production cost. The subjects were a random sample of poultry farmers and industry partnerships in a developing country with a high level of broiler consumption. A study was conducted on internal and external factors regarding strategic decisions. The strategy method stages include finding alternatives and framing strategies using the strength, weakness, opportunity, and threats (SWOT) matrix and quantitative strategic planning matrix (QSPM), respectively. The SWOT matrix analyzes the strategies that can be conducted in this case study: market penetration and product development. QSPM explained that the best strategic decisions were an intensive approach to quality animal feed producers, quality management training for poultry farmers and internal company parties, and business diversification for broiler processed products. Apart from preserving biodiversity, hopefully, this study will contribute to food security and improve the local communities’ economy.
Sequences of Trypsin Inhibitor Gene on Jatropha curcas L
Authors: Primandiri P R , Amin M , Zubaidah S , Maftuchah  , Santoso A M
Published: May 2021

Trypsin Inhibitor (TI) is one of unique gene which involved in plant protection from biotic and abiotic defense. The exploration variation of TI gene sequence is crucially needed asbasic reference, especially in J. curcas to support national biodiesel production. TI gene in J. curcas has been successfully isolated and sequenced form some crossed numbers (number 5 (SP-8 X SP-16), 6 (SP-8 X SP-38), 7 (SP-33 X HS-49), and 18 (SM-35 X SP-38) of J. curcas. Fourth sequence samples compared with two prime accessions (IP3A and IP3P). TI gene was successfully isolated, sequensed (598-604 bp), and aligned using BioEdit Sequence Alignment Editor 7.2.5 program. There were insertion and substitutionthat occur in all samples that lead to differences TI gene sequences. There are 11 siteswas inserted. Sample 18 and 7 only inserted at one site only. Substitution has been occured at 14 sites, 35.71% (5 sites) as transition and 64.29% as transvertion. Both ofinversion and substitution in some sites leads to change in the proportion of nucleic acid ofTI genes. Further analysis is needed to determine the impact of IT gene variation on J. curcas physiological response in environmental defense.