bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment of endometriosis with dienogest in combination with traditional Chinese medicine: A systematic review and meta-analysis
Authors: Wu Yu’e , Liu Yujie , Jia Huanhuan , Luo Chao , Chen Huan
Published: November 2022

Background

Endometriosis is now considered to be a systemic disease rather than a disease that primarily affects the pelvis. Dienogest (DNG) has unique advantages in the treatment of endometriosis, but it also has side effects. Alternatively, Traditional Chinese Medicine (TCM) has been used for over 2000 years in the treatment and prevention of disease and growing numbers of Chinese scholars are experimenting with the combined use of Dienogest and TCM for endometriosis treatment.

Objectives

This review evaluated the efficacy and safety of TCM in combination with Dienogest in the treatment of endometriosis through meta-analysis.

Methods

MEDLINE, Embase, the Cochrane Library, PubMed, Web of Science, China National Knowledge Infrastructure, Journal Integration Platform, and Wanfang were used in literature searches, with a deadline of May 31, 2022. Literature quality was assessed using the Cochrane Collaboration “risk of bias” (ROB2) tool, and the “meta” package of R software v.4.1 was used for meta-analysis. Dichotomous variables and continuous variables were assessed using the relative risk (RR) and 95% confidence intervals (95% CI); standard mean differences (MD) and 95% CI, respectively.

Results

Twelve human randomized controlled trials (RCTs) and one retrospective study, all 13 written in the Chinese language, were included in the meta-analysis (720 experiments and 719 controls). The result indicated that TCM plus Dienogest was superior to Dienogest/TCM alone in increasing the cure rates (RR = 1.3780; 95% CI, 1.1058, 1.7172; P = 0.0043), remarkable effect rate (RR = 1.3389; 95% CI, 1.1829, 1.5154; P < 0.0001), invalid rate (RR = 0.2299; 95% CI, 0.1591, 0.3322; P < 0.0001), and rate of adverse effects (RR = 0.6177; 95% CI, 0.4288, 0.8899; P = 0.0097). The same conclusion was drawn from the subgroup analysis.

Conclusion

Results suggest that TCM combined with Dienogest is superior to Dienogest or TCM alone and can be used as a complementary treatment for endometriosis. TCMs have potential to improve clinical efficacy and reduce the side effects of Dienogest. This study was financially supported by Annual Science and Technology Steering Plan Project of Zhuzhou. PROSPERO has registered our meta-analysis as CRD42022339518 (https://www.crd.york.ac.uk/prospero/record_email.php).
Endoscopic lateral neck dissection via the breast and transoral approaches for papillary thyroid carcinoma: A preliminary report
Authors: Kuang Penghao , Wang Yuanyuan , Wu Guoyang , Luo Yezhe , Fu Jinbo , Yan Wei , Lin Suqiong , Hong Xiaoquan , Lin Fusheng , Lin Ende , Fu Yilong
Published: November 2022

Purpose

Complete lymph node dissection is essential for the management of papillary thyroid carcinoma (PTC) with lymph node metastasis (LNM). This work aimed to describe the feasibility of endoscopic lateral neck dissection via the breast and transoral approach (ELNDBTOA) in PTC patients and the necessity of the addition of the transoral approach.

Methods

We included 13 patients with PTC and suspected lateral LNM who underwent ELNDBTOA at the Zhongshan Hospital, Xiamen University. Total thyroidectomy, ipsilateral central lymph node dissection, and selective neck dissection (levels IIA, IIB, III, and IV) were performed endoscopically via the breast approach. Residual lymph nodes were further dissected via the transoral approach.

Results

The mean operation time was 362.1 ± 73.5 min. In the lateral neck compartments, the mean number of retrieved lymph nodes was 36.6 ± 23.8, and the mean number of positive lymph nodes was 6.8 ± 4.7. In further dissection via the transoral approach, lymph nodes in the lateral neck compartment were obtained in nine patients (9/13, 69.2%), and three patients (3/13, 23.1%) had confirmed lateral neck metastases. Transient hypocalcemia occurred in two patients (2/13, 15.4%), and three patients (3/13, 23.1%) developed transient skin numbness in the mandibular area. No other major complications were observed. There was no evidence of local recurrence or distant metastasis during the follow-up period (range, 24–87 months). All patients were satisfied with the good cosmetic outcome.

Conclusion

ELNDBTOA is an option with proven feasibility for select PTC patients with LNM, and the addition of the transoral approach is necessary to ensure complete dissection.
A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematode Ascaris
Authors: Evangelista Francisco M. D. , van Vliet Arnoud H. M. , Lawton Scott P. , Betson Martha
Published: November 2022

Ascariasis is the most prevalent helminthic disease affecting both humans and pigs and is caused by the roundworms Ascaris lumbricoides and Ascaris suum. While preventive chemotherapy continues to be the most common control method, recent reports of anthelminthic resistance highlight the need for development of a vaccine against ascariasis. The aim of this study was to use a reverse vaccinology approach to identify potential vaccine candidates for Ascaris. Three Ascaris proteomes predicted from whole-genome sequences were analyzed. Candidate proteins were identified using open-access bioinformatic tools (e.g., Vacceed, VaxiJen, Bepipred 2.0) which test for different characteristics such as sub-cellular location, T-cell and B-cell molecular binding, antigenicity, allergenicity and phylogenetic relationship with other nematode proteins. From over 100,000 protein sequences analyzed, four transmembrane proteins were predicted to be non-allergen antigens and potential vaccine candidates. The four proteins are a Piezo protein, two voltage-dependent calcium channels and a protocadherin-like protein, are all expressed in either the muscle or ovaries of both Ascaris species, and all contained high affinity epitopes for T-cells and B-cells. The use of a reverse vaccinology approach allowed the prediction of four new potential vaccination targets against ascariasis in humans and pigs. These targets can now be further tested in in vitro and in vivo assays to prove efficacy in both pigs and humans.
Intratumoral injection of holmium-166 microspheres as neoadjuvant therapy of soft tissue sarcomas in dogs
Authors: Morsink Nino Chiron , Nijsen Johannes Frank Wilhelmus , Grinwis Guillaume Cornelis Maria , Hesselink Jan Willem , Kirpensteijn Jolle , van Nimwegen Sebastiaan Alexander
Published: November 2022

Introduction

Minimally invasive microbrachytherapy is in development to treat solid tumors by intratumoral injection of (radioactive) holmium-166 (166Ho) microspheres (MS). A high local dose can be administered with minimal damage to surrounding tissue because of the short soft tissue penetration depth of 166Ho beta radiation. We aimed to prospectively evaluate the safety and efficacy of 166Ho microbrachytherapy in client-owned canine patients with soft tissue sarcomas (STS).

Methods

We included seven dogs with STS not suitable for local excision due to tumor size and/or location. 166HoMS were suspended in a carrier fluid and multiple needle-injections were performed in predetermined tumor segments to maximize tumor coverage. Tumor response was evaluated using 3D caliper and CT measurements. Follow-up further included monitoring for potential side effects and registration of subsequent treatments and survival, until at least two years after treatment.

Results

Delivered radioactive doses ranged from 70 to 969 Gy resulting in a mean tumor volume reduction of 49.0 ± 21.3% after 33 ± 25 days. Treatment-related side effects consisted of local necrosis (n = 1) and ulceration of the skin covering the tumor (n = 1), which resolved with basic wound care, and surgical excision of residual tumor, respectively. Residual tumor was surgically resected in six patients after 22–93 days. After a mean follow-up of 1,005 days, four patients were alive, two patients were euthanized because of unrelated causes, and one patient was euthanized because of disease progression after the owner(s) declined subsequent surgical treatment.

Conclusion

166Ho microbrachytherapy was a safe and effective neoadjuvant treatment option for canine patients with STS.
Altered recruitment of Sp isoforms to HIV-1 long terminal repeat between differentiated monoblastic cell lines and primary monocyte-derived macrophages
Authors: McAllister John J. , Dahiya Satinder , Berman Rachel , Collins Mackenzie , Nonnemacher Michael R. , Burdo Tricia H. , Wigdahl Brian
Published: November 2022

Human immunodeficiency virus type 1 (HIV-1) transcription in cells of the monocyte-macrophage lineage is regulated by interactions between the HIV-1 long terminal repeat (LTR) and a variety of host cell and viral proteins. Binding of the Sp family of transcription factors (TFs) to the G/C box array of the LTR governs both basal as well as activated LTR-directed transcriptional activity. The effect of monocytic differentiation on Sp factor binding and transactivation was examined with respect to the HIV-1 LTR. The binding of Sp1, full-length Sp3 and truncated Sp3 to a high affinity HIV-1 Sp element was specifically investigated and results showed that Sp1 binding increased relative to the binding of the sum of full-length and truncated Sp3 binding following chemically-induced monocytic differentiation in monoblastic (U-937, THP-1) and myelomonocytic (HL-60) cells. In addition, Sp binding ratios from PMA-induced cell lines were shown to more closely approximate those derived from primary monocyte-derived macrophages (MDMs) than did ratios derived from uninduced cell lines. The altered Sp binding phenotype associated with changes in the transcriptional activation mediated by the HIV-1 G/C box array. Additionally, analysis of post-translational modifications on Sp1 and Sp3 revealed a loss of phosphorylation on serine and threonine residues with chemically-induced differentiation indicating that the activity of Sp factors is additionally regulated at the level of post-translational modifications (PTMs).
El Proceso de Aceptación y Adaptación a la Bisexualidad a través de la Terapia Cognitivo-Conductual con Enfoque Afirmativo
Authors: Rodríguez Riesco Lorenzo , Vivas Sebastián , Ruiz-Duet Ana M. , Pérez-Calvo Cristina
Published: November 2022

Las personas del colectivo LGBTIQ+ vivencian en su día a día diversas situaciones de estigmatización social, repercutiendo en su bienestar emocional y su salud mental. Estas actitudes negativas pueden moderar los sentimientos y percepciones de aquellas que pasan a formar parte del colectivo, como ocurre con la bisexualidad. En este estudio se presenta un acercamiento cognitivo-conductual con enfoque afirmativo en un caso de adaptación y aceptación de una usuaria que recientemente “redescubre” su orientación bisexual. A lo largo de 12 sesiones, a través de la psicoeducación, la restructuración cognitiva, el entrenamiento en asertividad y la exposición; se consigue reducir en la usuaria las diferentes problemáticas detectadas. Se consigue reducir los sentimientos de tristeza y ansiedad, se reduce el estrés percibido en situaciones problemáticas y se optimizan sus recursos en gestión emocional, obteniendo mejores resultados cuantitativos en la Escala de Evaluación de Resultados. Se concluye que este tipo de abordajes psicoterapéuticos son efectivos.
Apropiación de Sí
Authors: Rodríguez Lizana Danilo Andrés , Duarte Soto A. Javiera
Published: November 2022

Que el sujeto consultante desarrolle -a lo largo de su tratamiento- una comprensión de los fundamentos subjetivos de su padecimiento, es considerado como un variable relevante por la investigación en proceso psicoterapéutico, y una cualidad transversal en modelos psicoterapéuticos fundamentados en el paradigma Constructivista. Desde el método psicoterapéutico Posracionalista Fenomenológico Hermenéutico, a este fenómeno se le denomina: Apropiación de sí. Al ser un fenómeno que ocurre en la esfera privada de la experiencia, se estima relevante su estudio desde una metodología que acceda a éste, desde la primera persona que lo experimenta. De esta manera, se realizó un estudio cualitativo, de alcance descriptivo, utilizando la metodología de Entrevista Micro-fenomenológica. Se recolectó y analizó, bajo esta metodología, los resultados de diez entrevistas a sujetos que hayan cursado una psicoterapia de corte constructivista. Los resultados obtenidos permiten comprender la apropiación de sí como un fenómeno transversal en los sujetos entrevistados, caracterizándose como una transformación en el modo de acceder comprensiblemente a su propio padecer. Esta inteligibilidad permite una posición en propiedad respecto a sus circunstancias, que orienta a la acción renovadora, abriendo posibilidades a través de la transformación personal.
Formulación de Focos y Articulación de Proyectos Terapéuticos en Terapia Focalizada en la Emoción: Una Aproximación basada en el Análisis de Tareas
Authors: Caro García Ciro
Published: November 2022

El trabajo comienza con la revisión de la Formulación de Caso (FC) como acción social, en el sentido de una actividad cargada de intenciones y “no inocente” que, por tanto, requiere un cuidado especial respecto a los procesos que comprende. A continuación, se presenta brevemente el modelo de FC de la Terapia Focalizada en la Emoción (TFE) y se plantea el siguiente problema: la ausencia de un modelo explícito de lo que representa la mejor ejecución del proceso de co-construir un foco y acordar un proyecto terapéutico en TFE. Dicho problema es analizado mediante la revisión crítica de las dimensiones de la FC (proceso, producto y experiencia del cliente) así como su naturaleza como práctica de gestión del conocimiento en psicoterapia. A partir de ahí, se desarrollan los conceptos complementarios de “foco” y “proyecto terapéutico”, a fin de dotar de fundamento a una respuesta al problema planteado. Para responder, se parte de la consideración del proceso que se desea modelizar como una “macro-tarea terapéutica” orientada a la coordinación intersubjetiva entre cliente y terapeuta, y se conduce la fase inicial del Análisis de Tareas. En la sección de resultados se ofrece un modelo racional-hipotético del proceso de co-construir un foco y acordar un proyecto terapéutico en TFE, así como un manual de la tarea y la correspondiente escala de grados de resolución. Finalmente, se discuten los tres productos en términos de su valor general como recursos para la práctica integrativa en psicoterapia y el desarrollo competencial de los terapeutas.
Domination and Independent Domination in Extended Supergrid Graphs
Authors: Chen Jong-Shin , Hung Ruo-Wei , Keshavarz-Kohjerdi Fatemeh , Huang Yung-Fa
Published: November 2022

Supergrid graphs are derived by computing stitch paths for computerized embroidery machines. In the past, we have studied the Hamiltonian-related properties of supergrid graphs and their subclasses of graphs. In this paper, we propose a generalized graph class for supergrid graphs called extended supergrid graphs. Extended supergrid graphs include grid graphs, supergrid graphs, diagonal supergrid graphs, and triangular supergrid graphs as subclasses of graphs. In this paper, we study the problems of domination and independent domination on extended supergrid graphs. A dominating set of a graph is the subset of vertices on it, such that every vertex of the graph is in this set or adjacent to at least a vertex of this set. If any two vertices in a dominating set are not adjacent, this is called an independent dominating set. Domination and independent domination problems find a dominating set and an independent dominating set with the least number of vertices on a graph, respectively. The domination and independent domination set problems on grid graphs are known to be NP-complete, meaning that these two problems on extended supergrid graphs are also NP-complete. However, the complexities of these two problems in other subclasses of graphs remain unknown. In this paper, we first prove that these two problems on diagonal supergrid graphs are NP-complete, then, by a simple extension, we prove that these two problems on supergrid graphs and triangular supergrid graphs are also NP-complete. In addition, these two problems on rectangular supergrid graphs are known to be linearly solvable; however, the complexities of these two problems on rectangular triangular-supergrid graphs remain unknown. This paper provides tight upper bounds on the sizes of the minimum dominating and independent dominating sets for rectangular triangular-supergrid graphs.
Deep Learning Models for Yoga Pose Monitoring
Authors: Swain Debabrata , Satapathy Santosh , Acharya Biswaranjan , Shukla Madhu , Gerogiannis Vassilis C. , Kanavos Andreas , Giakovis Dimitris
Published: November 2022

Activity recognition is the process of continuously monitoring a person’s activity and movement. Human posture recognition can be utilized to assemble a self-guidance practice framework that permits individuals to accurately learn and rehearse yoga postures without getting help from anyone else. With the use of deep learning algorithms, we propose an approach for the efficient detection and recognition of various yoga poses. The chosen dataset consists of 85 videos with 6 yoga postures performed by 15 participants, where the keypoints of users are extracted using the Mediapipe library. A combination of Convolutional Neural Network (CNN) and Long Short-Term Memory (LSTM) has been employed for yoga pose recognition through real-time monitored videos as a deep learning model. Specifically, the CNN layer is used for the extraction of features from the keypoints and the following LSTM layer understands the occurrence of sequence of frames for predictions to be implemented. In following, the poses are classified as correct or incorrect; if a correct pose is identified, then the system will provide user the corresponding feedback through text/speech. This paper combines machine learning foundations with data structures as the synergy between these two areas can be established in the sense that machine learning techniques and especially deep learning can efficiently recognize data schemas and make them interoperable.
Evolutionary Algorithm-Based Iterated Local Search Hyper-Heuristic for Combinatorial Optimization Problems
Authors: Adubi Stephen A. , Oladipupo Olufunke O. , Olugbara Oludayo O.
Published: November 2022

Hyper-heuristics are widely used for solving numerous complex computational search problems because of their intrinsic capability to generalize across problem domains. The fair-share iterated local search is one of the most successful hyper-heuristics for cross-domain search with outstanding performances on six problem domains. However, it has recorded low performances on three supplementary problems, namely knapsack, quadratic assignment, and maximum-cut problems, which undermines its credibility across problem domains. The purpose of this study was to design an evolutionary algorithm-based iterated local search (EA-ILS) hyper-heuristic that applies a novel mutation operator to control the selection of perturbative low-level heuristics in searching for optimal sequences for performance improvement. The algorithm was compared to existing ones in the hyper-heuristics flexible (HyFlex) framework to demonstrate its performance across the problem domains of knapsack, quadratic assignment, and maximum cut. The comparative results have shown that the EA-ILS hyper-heuristic can obtain the best median objective function values on 22 out of 30 instances in the HyFlex framework. Moreover, it has achieved superiority in its generalization capability when compared to the reported top-performing hyper-heuristic algorithms.
Stable Evaluation of 3D Zernike Moments for Surface Meshes
Authors: Houdayer Jérôme , Koehl Patrice
Published: November 2022

The 3D Zernike polynomials form an orthonormal basis of the unit ball. The associated 3D Zernike moments have been successfully applied for 3D shape recognition; they are popular in structural biology for comparing protein structures and properties. Many algorithms have been proposed for computing those moments, starting from a voxel-based representation or from a surface based geometric mesh of the shape. As the order of the 3D Zernike moments increases, however, those algorithms suffer from decrease in computational efficiency and more importantly from numerical accuracy. In this paper, new algorithms are proposed to compute the 3D Zernike moments of a homogeneous shape defined by an unstructured triangulation of its surface that remove those numerical inaccuracies. These algorithms rely on the analytical integration of the moments on tetrahedra defined by the surface triangles and a central point and on a set of novel recurrent relationships between the corresponding integrals. The mathematical basis and implementation details of the algorithms are presented and their numerical stability is evaluated.
An Evolutionary, Gradient-Free, Query-Efficient, Black-Box Algorithm for Generating Adversarial Instances in Deep Convolutional Neural Networks
Authors: Lapid Raz , Haramaty Zvika , Sipper Moshe
Published: November 2022

Deep neural networks (DNNs) are sensitive to adversarial data in a variety of scenarios, including the black-box scenario, where the attacker is only allowed to query the trained model and receive an output. Existing black-box methods for creating adversarial instances are costly, often using gradient estimation or training a replacement network. This paper introduces Query-Efficient Evolutionary Attack—QuEry Attack—an untargeted, score-based, black-box attack. QuEry Attack is based on a novel objective function that can be used in gradient-free optimization problems. The attack only requires access to the output logits of the classifier and is thus not affected by gradient masking. No additional information is needed, rendering our method more suitable to real-life situations. We test its performance with three different, commonly used, pretrained image-classifications models—Inception-v3, ResNet-50, and VGG-16-BN—against three benchmark datasets: MNIST, CIFAR10 and ImageNet. Furthermore, we evaluate QuEry Attack’s performance on non-differential transformation defenses and robust models. Our results demonstrate the superior performance of QuEry Attack, both in terms of accuracy score and query efficiency.
Special Issue on Algorithms in Planning and Operation of Power Systems
Authors: Rivera Sergio R.
Published: November 2022

Optimal planning and secure grid operation are new challenges facing modern power systems [...]
Correlation between Seismic Waves Velocity Changes and the Occurrence of Moderate Earthquakes at the Bending of the Eastern Carpathians (Vrancea)
Authors: Placinta Anica-Otilia , Borleanu Felix , Moldovan Iren-Adelina , Coman Alina
Published: November 2022

Seismic velocity is the geophysical property that has a key role in characterizing dynamic processes and the state of the stress around the faults, providing valuable information regarding the change in the tectonic regime. The stress in the crust is an important indicator of the possible occurrence of a major earthquake, and the variation of seismic velocities, in time, can provide a clearer picture on the tectonic processes taking place in the region. In the crust, velocities change before, during, and after earthquakes through several mechanisms related to fault deformations, pore pressure, stress changes, and recovery processes. In this study, we investigate the possible correlation between the changes of seismic velocities (Vp/Vs) in time and the occurrence of moderate size crustal and intermediate depth earthquakes from the Vrancea region. Our findings show that there are no significant variations in Vp/Vs for the intermediate depth earthquakes, while crustal events have decreased seismic activity prior to the main earthquake and no high Vp/Vs anomalies. Our results indicate key aspects, and such analyses should be carried out in real-time to continuously explore any unusual pattern pointed out by the seismic velocity changes. Vp/Vs and their standard errors can also be used to describe seismic activity patterns that shape the tectonic evolution of the area.
Trait Variation between Two Wild Specimens of Pleurotus ostreatus and Their Progeny in the Context of Usefulness in Nematode Control
Authors: Kudrys Paweł , Nabrdalik Małgorzata , Hendel Patrycja , Kolasa-Więcek Alicja , Moliszewska Ewa
Published: November 2022

Pleurotus ostreatus is one of the most widespread mushrooms in the world. It is a valuable and widely cultivated edible mushroom with nematicidal properties. The mycelium of this mushroom produces a toxin that paralyzes nematodes, after which the nematode becomes infected with the filament, resulting in its death. This feature can be put to practical use as a natural nematicidal agent. In this paper, we have described studies on two wild strains of P. ostreatus (PO1, PO2) and the monokaryotic progeny obtained from their fruiting bodies. Monokaryons were crossed with each other within the progeny of one strain (PO1xPO1; PO2xPO2) and of two different strains (PO2xPO1). The growth conditions, morphological and molecular characteristics, toxicity and ability to form hyphal knobs of the maternal strains, monokaryons and those obtained by crossing were studied. The toxicity of mycelium was tested against the model organism Caenorhabditis elegans. The results of the study allowed the selection of five progeny heterokaryotic mycelia with the best parameters useful for in-vitro control of nematodes.
Detection of Planting Systems in Olive Groves Based on Open-Source, High-Resolution Images and Convolutional Neural Networks
Authors: Martínez-Ruedas Cristina , Yanes-Luis Samuel , Díaz-Cabrera Juan Manuel , Gutiérrez-Reina Daniel , Linares-Burgos Rafael , Castillejo-González Isabel Luisa
Published: November 2022

This paper aims to evaluate whether an automatic analysis with deep learning convolutional neural networks techniques offer the ability to efficiently identify olive groves with different intensification patterns by using very high-resolution aerial orthophotographs. First, a sub-image crop classification was carried out. To standardize the size and increase the number of samples of the data training (DT), the crop images were divided into mini-crops (sub-images) using segmentation techniques, which used a different threshold and stride size to consider the mini-crop as suitable for the analysis. The four scenarios evaluated discriminated the sub-images efficiently (accuracies higher than 0.8), obtaining the largest sub-images (H = 120, W = 120) for the highest average accuracy (0.957). The super-intensive olive plantings were the easiest to classify for most of the sub-image sizes. Nevertheless, although traditional olive groves were discriminated accurately, too, the most difficult task was to distinguish between the intensive plantings and the traditional ones. A second phase of the proposed system was to predict the crop at farm-level based on the most frequent class detected in the sub-images of each crop. The results obtained at farm level were slightly lower than at the sub-images level, reaching the highest accuracy (0.826) with an intermediate size image (H = 80, W = 80). Thus, the convolutional neural networks proposed made it possible to automate the classification and discriminate accurately among traditional, intensive, and super-intensive planting systems.
Genotypic and Environmental Influence on Fresh Rhizome Yield of Turmeric (Curcuma longa L.)
Authors: Tavares Kylie , Kirk Emilie , Motomura-Wages Sharon , Calpito Justin , Bingham Jon-Paul , Ahmad Amjad A. , Flanagan Kevin , Uyeda Jensen , Kantar Michael B. , Radovich Theodore J. K.
Published: November 2022

Turmeric (Curcuma longa) and related Curcuma species have been used traditionally in India, China, Hawaii, and other cultures for millennia. Today they are used around the world for spice, medicine, dye, and religious purposes. Recent biomedical studies have corroborated the long-known traditional medicinal values of turmeric and its constituent curcuminoid compounds, which have anti-inflammatory, antioxidant, and anticarcinogenic properties. As part of statewide research and extension efforts to support an expanding turmeric industry, we examined yield of 14 accessions across different climatic zones in Hawaii to observe and describe Genotype × Environmental influences. Fresh turmeric yield differed significantly among genotypes. The overall yields observed in this work ranged 11.3–57.22 t ha−1 and generally agree with those in the literature. Data from the different sites suggest that fertility and water management are able to mitigate moderate stress imposed by climate change within a certain range, but suboptimal temperatures associated with high elevation in the tropics (>1000 m) are an important driver of lower yields. This suggests that high yielding turmeric varieties may possess wide adaptability and may perform well across diverse environments. However, site-specific evaluations will still be necessary, particularly in environments outside turmeric’s environmental optima and in the presence of high pest pressure.
Molecular Epidemiology of Blastocystis in Confined Slow Lorises, Macaques, and Gibbons
Authors: Ni Qingyong , Dong Shasha , Fan Yumai , Wan Wen , Teng Ping , Zhu Shaobo , Liang Xiaobi , Xu Huailiang , Yao Yongfang , Zhang Mingwang , Xie Meng
Published: November 2022

Blastocystis sp. is a common intestinal anaerobic parasite infecting non-human primates and many other animals. This taxon threatens the health of NHPs due to its high genetic diversity, impeding efforts to improve confined management and subsequent conservation practices. This study collected 100 and 154 fecal samples from captive macaques, gibbons, and slow lorises in the summer and winter, respectively. The Blastocystis infection, its gene subtypes, and its zoonotic potential based on small subunit ribosomal RNA (SSU rRNA) were analyzed. The prevalence of Blastocystis in the three primate genera was 57.79% (89/154) in the summer (2021) and 29.00% (29/100) in the winter (2020). Four zoonotic subtypes—ST1, ST2, ST3, and ST4—were identified. ST2 was the most prevalent subtype, suggesting that these animals may serve as reservoirs for pathogens of human Blastocystis infections. The macaques showed a more significant variation in Blastocystis infection between seasons than gibbons and slow lorises. The slow lorises in small cages and enclosure areas were potentially more infected by Blastocystis in the summer, indicating that inappropriate captive management may have detrimental effects on their health.
Re-Thinking Felid–Human Entanglements through the Lenses of Compassionate Conservation and Multispecies Studies
Authors: Steinhardt Margarita , Pratt Susanne , Ramp Daniel
Published: November 2022

With many felid species in widespread decline, re-thinking the messy felid–human entanglements is a necessary step for fostering coexistence as current conservation frameworks centered on human exceptionalism and widespread violence toward wild animals are conspicuously failing felids. This paper argues for fostering a critical awareness of how we understand our relationships with nonhuman animals, particularly in the context of conservation. We bring two distinct but related interdisciplinary fields into a dialogue to critically question the values and conceptual assumptions that frame the practices of felid conservation today. Compassionate conservation and multispecies studies share many synergies and conceptual overlaps despite emerging from different academic domains. We identified four key areas for further exploration: (1) A shift in emphasis from practices of killing to the underlying assumptions that make forms of killing permissible and ethically unproblematic. (2) Re-engagement with individuals, not just species, in conservation settings. (3) Unsettling human exceptionalism through an emphasis on the agency of animals and an ethic involving compassion. (4) Acknowledging the ways in which humans co-become with other animals and cultivating relationships of multispecies cohabitation and flourishing.
Garlic and Its Bioactive Compounds: Implications for Methane Emissions and Ruminant Nutrition
Authors: Sari Nurul Fitri , Ray Partha , Rymer Caroline , Kliem Kirsty E. , Stergiadis Sokratis
Published: November 2022

Methane (CH4) emission from enteric fermentation of ruminant livestock is a source of greenhouse gases (GHG) and has become a significant concern for global warming. Enteric methane emission is also associated with poor feed efficiency. Therefore, research has focused on identifying dietary mitigation strategies to decrease CH4 emissions from ruminants. In recent years, plant-derived bioactive compounds have been investigated for their potential to reduce CH4 emissions from ruminant livestock. The organosulphur compounds of garlic have been observed to decrease CH4 emission and increase propionate concentration in anaerobic fermentations (in vitro) and in the rumen (in vivo). However, the mode of action of CH4 reduction is not completely clear, and the response in vivo is inconsistent. It might be affected by variations in the concentration and effect of individual substances in garlic. The composition of the diet that is being fed to the animal may also contribute to these differences. This review provides a summary of the effect of garlic and its bioactive compounds on CH4 emissions by ruminants. Additionally, this review aims to provide insight into garlic and its bioactive compounds in terms of enteric CH4 mitigation efficacy, consistency in afficacy, possible mode of action, and safety deriving data from both in vivo and in vitro studies.
The Influence of the Gut Microbiome in Paediatric Cancer Origin and Treatment
Authors: Sági Viktória , Makra Nóra , Csoszánszki Noémi , Decmann Abel , Szabó Dóra , Garami Miklós
Published: November 2022

Knowledge of the complexity of the gut microbiota is expanding, and its importance in physiological processes and disease development is widely studied. The aim of this review is to present the most relevant and recent research on the associations between gut microbiota and oncologic disease. Recently, a number of associations between the gut microbiome and neoplasms—regarding tumorigenesis, prognosis and therapeutic efficacy—have been reported. The effects of the gut microbiome on these processes are via the direct and indirect immunomodulating effects of bacteria. Studies have been done mainly in adult populations, where its effect on immunomodulating therapies was unambiguous. In paediatric populations, however, due to the low number of cases and the complex therapeutic approaches, there have been only a few studies. Among them, children with acute lymphoblastic leukaemia were mainly involved. Significant alterations in the abundance of certain bacteria were associated with altered therapeutic responses. Regarding solid tumours, studies with low case numbers have been reported; no significant discoveries have been described so far. In the future, studies with larger cohorts are needed in order to better understand the associations between bacteria and neoplasms and to improve prognosis in the paediatric oncologic population.
Comparable Benefits of Stingless Bee Honey and Caffeic Acid in Mitigating the Negative Effects of Metabolic Syndrome on the Brain
Authors: Muhammad Abdul Kadar Nellysha Namela , Ahmad Fairus , Teoh Seong Lin , Yahaya Mohamad Fairuz
Published: November 2022

There is mounting evidence that metabolic syndrome (MetS) contributes to the development of neurodegenerative disorders such as Alzheimer’s disease. Honey, which has been used for generations, is high in antioxidants and has been demonstrated to benefit the brain and mental health by reducing oxidative stress and boosting cognitive outcomes. Honey from the stingless bees of Heterotrigona itama has been found to have higher phenolic content compared to other types of honeys. The aim of this study is to investigate the effects of stingless bee honey (SBH) supplementation and to compare it with a pure form of antioxidant, caffeic acid (CA), on MetS parameters and inflammatory markers in the brains of MetS-induced rats. A total of 32 male Wistar rats were divided equally into groups of control, high-carbohydrate high-fructose (HCHF) diet (MetS), HCHF + SBH supplemented (1 g/kg) (SBH), and HCHF + CA supplemented (10 mg/kg) (CA) groups. The total duration for SBH and CA supplementation was eight weeks. The HCHF diet was found to promote hypertension, hyperglycemia, and hypertriglyceridemia, and to increase brain TNF-α levels. Supplementation with SBH and CA significantly reversed (p < 0.05) the hyperglycemic and hypertensive effects of the HCHF diet. Although both supplemented groups showed no significant changes to serum HDL or TG, SBH significantly reduced (p < 0.05) brain TNF-α levels and increased (p < 0.05) brain BDNF levels. Immunohistochemistry investigations of neurogenesis (EdU) and apoptosis (TUNEL) on the cornu Ammonis 1 (CA1) and dentate gyrus (DG) areas of the hippocampus showed no changes with SBH and CA supplementation compared to the control. These findings suggest that SBH and CA have the potential to mitigate HCHF-induced MetS effects and possess neuroprotective abilities.
Spatial Distribution, Antioxidant Capacity, and Spore Germination-Promoting Effect of Bibenzyls from Marchantia polymorpha
Authors: Zhang Jiao-Zhen , Wang Chan , Zhu Ting-Ting , Fu Jie , Tan Hui , Zhang Cheng-Min , Cheng Ai-Xia , Lou Hong-Xiang
Published: November 2022

Liverworts, considered to be the first plant type to successfully make the transition from water to land, can resist different oxidative stress. As characteristic constituents of liverworts, the bibenzyls are efficient antioxidants. In this study, spatial distributions of the bibenzyls within Marchantia polymorpha L., the model species of liverworts, were mapped using airflow-assisted desorption electrospray ionization imaging mass spectrometry. Bibenzyls were found to largely exist in the female receptacle of M. polymorpha, where lunularic acid was found to focus in the central region and bisbibenzyls were enriched in the periphery. The region-specific gene expression and antioxidant activities were characterized. In line with the spatial feature of bibenzyls, higher MpSTCS1A and Mp4CL expression levels and antioxidant ability were exhibited in the archegoniophore. The expression level of MpSTCS1A, and the content of total phenolic acid was increased after UV-B irradiation, suggesting bibenzyls play an important role in UV-B tolerance. Moreover, lunularic acid and extract of archegoniophore at a certain concentration can stimulate the spore germination under normal conditions and UV-B stress. These works broaden our understanding of the significance of bibenzyls in spore propagation and environmental adaptation.
The Role of the Thioredoxin System in Brain Diseases
Authors: Bjørklund Geir , Zou Lili , Peana Massimiliano , Chasapis Christos T. , Hangan Tony , Lu Jun , Maes Michael
Published: November 2022

The thioredoxin system, consisting of thioredoxin (Trx), thioredoxin reductase (TrxR), and NADPH, plays a fundamental role in the control of antioxidant defenses, cell proliferation, redox states, and apoptosis. Aberrations in the Trx system may lead to increased oxidative stress toxicity and neurodegenerative processes. This study reviews the role of the Trx system in the pathophysiology and treatment of Alzheimer’s, Parkinson’s and Huntington’s diseases, brain stroke, and multiple sclerosis. Trx system plays an important role in the pathophysiology of those disorders via multiple interactions through oxidative stress, apoptotic, neuro-immune, and pro-survival pathways. Multiple aberrations in Trx and TrxR systems related to other redox systems and their multiple reciprocal relationships with the neurodegenerative, neuro-inflammatory, and neuro-oxidative pathways are here analyzed. Genetic and environmental factors (nutrition, metals, and toxins) may impact the function of the Trx system, thereby contributing to neuropsychiatric disease. Aberrations in the Trx and TrxR systems could be a promising drug target to prevent and treat neurodegenerative, neuro-inflammatory, neuro-oxidative stress processes, and related brain disorders.
Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy
Authors: Alcaraz Miguel , Olivares Amparo , Andreu-Gálvez Marina , Achel Daniel Gyingiri , Mercado Ana María , Alcaraz-Saura Miguel
Published: November 2022

Carnosic acid (CA) is a phenolic diterpene characterized by its high antioxidant activity; it is used in industrial, cosmetic, and nutritional applications. We evaluated the radioprotective capacity of CA on cells directly exposed to X-rays and non-irradiated cells that received signals from X-ray treated cells (radiation induced bystander effect, RIBE). The genoprotective capacity was studied by in vivo and in vitro micronucleus assays. Radioprotective capacity was evaluated by clonogenic cell survival, MTT, apoptosis and intracellular glutathione assays comparing radiosensitive cells (human prostate epithelium, PNT2) with radioresistant cells (murine metastatic melanoma, B16F10). CA was found to exhibit a genoprotective capacity in cells exposed to radiation (p < 0.001) and in RIBE (p < 0.01). In PNT2 cells, considered as normal cells in our study, CA achieved 97% cell survival after exposure to 20 Gy of X-rays, eliminating 67% of radiation-induced cell death (p < 0.001), decreasing apoptosis (p < 0.001), and increasing the GSH/GSSH ratio (p < 0.01). However, the administration of CA to B16F10 cells decreased cell survival by 32%, increased cell death by 200% (p < 0.001) compared to irradiated cells, and increased cell death by 100% (p < 0.001) in RIBE bystander cells (p < 0.01). Furthermore, it increased apoptosis (p < 0.001) and decreased the GSH/GSSG ratio (p < 0.01), expressing a paradoxical radiosensitizing effect in these cells. Knowing the potential mechanisms of action of substances such as CA could help to create new applications that would protect healthy cells and exclusively damage neoplastic cells, thus presenting a new desirable strategy for cancer patients in need of radiotherapy.
ApoE3 vs. ApoE4 Astrocytes: A Detailed Analysis Provides New Insights into Differences in Cholesterol Homeostasis
Authors: Staurenghi Erica , Leoni Valerio , Lo Iacono Marco , Sottero Barbara , Testa Gabriella , Giannelli Serena , Leonarduzzi Gabriella , Gamba Paola
Published: November 2022

The strongest genetic risk factor for sporadic Alzheimer’s disease (AD) is the presence of the ε4 allele of the apolipoprotein E (ApoE) gene, the major apolipoprotein involved in brain cholesterol homeostasis. Being astrocytes the main producers of cholesterol and ApoE in the brain, we investigated the impact of the ApoE genotype on astrocyte cholesterol homeostasis. Two mouse astrocytic cell lines expressing the human ApoE3 or ApoE4 isoform were employed. Gas chromatography–mass spectrometry (GC-MS) analysis pointed out that the levels of total cholesterol, cholesterol precursors, and various oxysterols are altered in ApoE4 astrocytes. Moreover, the gene expression analysis of more than 40 lipid-related genes by qRT-PCR showed that certain genes are up-regulated (e.g., CYP27A1) and others down-regulated (e.g., PPARγ, LXRα) in ApoE4, compared to ApoE3 astrocytes. Beyond confirming the significant reduction in the levels of PPARγ, a key transcription factor involved in the maintenance of lipid homeostasis, Western blotting showed that both intracellular and secreted ApoE levels are altered in ApoE4 astrocytes, as well as the levels of receptors and transporters involved in lipid uptake/efflux (ABCA1, LDLR, LRP1, and ApoER2). Data showed that the ApoE genotype clearly affects astrocytic cholesterol homeostasis; however, further investigation is needed to clarify the mechanisms underlying these differences and the consequences on neighboring cells. Indeed, drug development aimed at restoring cholesterol homeostasis could be a potential strategy to counteract AD.
Carum carvi Modulates Acetaminophen-Induced Hepatotoxicity: Effects on TNF-α, NF-κB, and Caspases
Authors: Mir Tahir Maqbool , Rehman Muneeb U , Ashfaq Mohammad Khalid , Qamar Wajhul , Khan Rehan , Ali Aarif , Almuqbil Mansour , Alshehri Sultan , Sultana Sarwat
Published: November 2022

Carum carvi is a well-known herb traditionally used as a spice in Asian countries. Acetaminophen is a known marketed drug mainly used as an analgesic. It has been scientifically proven that consumption of acetaminophen (paracetamol) is associated with liver toxicity if taken in high doses without medical supervision. The present study evaluated the in vivo antioxidant and hepatoprotective efficacy of Carum carvi against acetaminophen-induced hepatotoxicity in Wistar rats. Our results demonstrate that Carum carvi, at doses (mg/kg) of 100 (D1) and 200 (D2), showed inhibitory properties for DNA-sugar damage, lipid peroxidation, DPPH scavenging, and increased reducing potential in a concentration-dependent manner. Our results also confirm that liver toxicity associated with paracetamol, such as depletion of reduced glutathione and antioxidant enzyme levels, as well as induction of cytochrome P450, oxidative stress, apoptosis, and inflammatory cytokines, was efficiently restored by Carum carvi treatment in rats. Moreover, the expression of redox-sensitive transcription factors, namely, NF-κB and TNF-α levels, was also modulated by Carum carvi in the rats. In summary, our study confirms that Carum carvi inhibits inflammation and oxidative stress, thereby protecting liver cells from paracetamol prompted hepatotoxicity.
Cable Force Identification of Two Bending Cable Networks with Arbitrary Boundary Conditions
Authors: Nguyen Van-Son , Wang Chung-Yue , Syamsi Muhammad Ibnu , Wang Hao-Lin
Published: November 2022

The determination of existing cable forces is essential for evaluating the performance of bridges. The vibration technique is preferred among available practical methods because it is simple, fast, and economical. To apply this method, many researchers introduced analytical and empirical formulas considering bending rigidity, cable length, and boundary conditions. Nevertheless, most existing procedures to determine cable forces are for single cables, which are unsuitable for cable networks. To measure the cable forces of the cable networks, engineers should remove any cross-ties before conducting field tests. However, the removal of cross-ties separates the cable network into two independent cables, which does not reflect the actual behavior of the cable network. Hence, to save time and improve accuracy, this paper introduces a method to directly identify the cable tensions of a two-cable network without removing any cross-ties. This technique can precisely estimate the cable forces of the two-bending cable networks with arbitrary boundary conditions with an error of less than 1.0 percent.
Light-Controlled Microbots in Biomedical Application: A Review
Authors: Jamil Md Faiyaz , Pokharel Mishal , Park Kihan
Published: November 2022

The advancement of micro-robotics in recent years has permitted a vast field of active research and application in the biomedical sector. Latest developments in microrobotics point to some ground-breaking work using light for manufacturing as well as actuation. Optical manipulation in three-dimensional space for living biological cells in a minimally invasive manner is crucial for different biomedical applications. This article attempts to provide an overview of the accomplishments and future possibilities of light-powered microbots. An overview of the feasibility of different fabrication techniques and control modalities is compared, along with prospective applications and design considerations of light-powered microbots. A variety of challenges that still prohibit polymeric light-powered microbots from attaining their full potential are pointed out, and viable ways to overcome such challenges are proposed. This study will help future researchers to study and develop the next generation of light-actuated microbots by overcoming the current limitations and challenges in fabrication, control, and design.