bluuebunny/crossref_metadata_2025 huggingface dataset:
An Energy-Saving Optimization Method of Dynamic Scheduling for Disassembly Line
Authors: Gao Yicong , Wang Qirui , Feng Yixiong , Zheng Hao , Zheng Bing , Tan Jianrong
Published: May 2018

Concerns have been increasing regarding the environmental sustainability of disassembly activities that take place in various recovery operations at end of life stage of products, and subsequently, disassembly activities have been gaining increased exposure. The disassembly line is a good choice for automated disassembly of end-of-life products. Although interest in addressing energy saving in manufacturing is rising, the study of incorporating energy consumption reduction and disassembly efficiency improvement into disassembly line balancing is still limited. The analysis, design, and balanced decision-making for disassembly lines are urgently needed in order to make the disassembly line as energy-saving as possible. In this paper, an energy-saving optimization method, which was used in scheduling workstation selection and the disassembly sequence for the disassembly line, is proposed. Energy consumption of each stage of the disassembling process was analyzed and modeled. Then, a mathematical model of the disassembly line balancing problem with energy saving considerations was formulated and the objective of energy consumption was integrated into the objectives of cost and working load in order to balance a disassembly line. Optimal solutions for the disassembly line balancing problem with an energy saving consideration were obtained by using an artificial bee colony algorithm, which was a feasible way of minimizing workstations, and ensuring similar working load, as well as minimizing energy consumption. Finally, a case study of disassembly line balancing for a typical driving system is presented to illustrate the proposed method.
Fe2(SO4)3-Catalyzed Levulinic Acid Esterification: Production of Fuel Bioadditives
Authors: Martins Fernanda , Rodrigues Fabio , Silva Marcio
Published: May 2018

In this work, we developed a route to synthesize fuel bioadditives based on simple and inexpensive commercial catalyst: iron(III) sulfate. This process is an alternative to the traditional Brønsted acid catalysts, avoiding steps to neutralize the catalyst, which generates a large amount of effluents and residues. High conversions (ca. 90%) and selectivity (90–97%) for alkyl esters of levulinic acid with carbon chain ranging from C6–C9 were obtained, when Fe2(SO4)3 was the catalyst. The role of each component catalyst system was studied, with a special attention on the reaction temperature, stoichiometry of reactants and catalyst concentration. We investigate the catalytic activity of others commercial transition metal salts (i.e., Fe2(SO4)3, FeCl3, CuSO4, FeSO4, MnSO4, NiSO4). Although soluble, the Fe2(SO4)3 catalyst was easily recovered by silica column chromatography, in the same step of products purification, and reused without loss activity. The use of renewable raw material and an efficient, inexpensive and recyclable catalyst are the main positive features of this process.
Effect of Wall Temperature on Acetylene Diffusion Flame–Wall Interaction Based on Optical Diagnostics and CFD Simulation
Authors: Liu Haifeng , Geng Chao , Yang Zhi , Cui Yanqing , Yao Mingfa
Published: May 2018

In order to elucidate the effect of wall temperature on a diffusion flame–wall interaction, an acetylene diffusion flame in a head-on quenching type was investigated. Direct photography, two-color thermometry, soot-LII (laser-induced incandescence), OH-LIF (laser-induced fluorescence) and numerical simulation with detailed reaction mechanisms were employed to find out the influence mechanism of wall temperature on near-wall combustion performance and emission characteristics. It is clearly shown through optical diagnostics and computation fluid dynamics (CFD) simulation that, compared with cold wall, the high temperature zone for hot wall becomes wider, and the smaller quenching layer is formed due to the higher wall heat flux. High-concentration soot emission is formed primarily near the outer flame far from the wall. CH2O, CO and HC emissions are decreased as wall temperature rises, while the formation of soot and A4 is increased. A diffusion flame–wall interaction structure is proposed to reveal the influence mechanism of wall temperature.
Economic and Environmental Analysis of Different District Heating Systems Aided by Geothermal Energy
Authors: Sáez Blázquez Cristina , Farfán Martín Arturo , Nieto Ignacio , González-Aguilera Diego
Published: May 2018

As a renewable energy source, geothermal energy can provide base-load power supply both for electricity and direct uses, such as space heating. Regarding this last use, in the present study, district heating systems aided by geothermal energy, the so-called geothermal district heating systems, are studied. Thus, three different options of a geothermal district heating system are evaluated and compared in terms of environmental and economic aspects with a traditional fossil installation. Calculations were carried out from a particular study case, a set of buildings located Province of León in the north of Spain. From real data of each of the assumptions considered, an exhaustive comparison among the different scenarios studied, was thoroughly made. Results revealed the most suitable option from an economic point of view but always considering the environmental impacts of each one. In this regard, the assumption of a district heating system totally supplied by geothermal energy clearly stands out from the rest of options. Thus, the manuscript main objective is to emphasise the advantages of these systems as they constitute the ideal solution from both the economic and environmental parameters analysed.
Incentive Systems for Risky Investment Decisions Under Unknown Preferences: Ortner et al. Revisited
Authors: Schosser Josef
Published: May 2018

Ortner et al. (Manage. Account. Res. 36(1):43–50, 2017) propose the State-Contingent Relative Benefit Cost Allocation Scheme as an incentive system for risky investment decisions. The note at hand reveals the information distribution implicitly assumed within the framework of this study. Based on this information distribution, both simpler and more powerful ways to induce consistency exist.
Towards a New Paradigm in Water Management: Cochabamba’s Water Agenda from an Ethical Approach
Authors: Bellaubi Francesc , Bustamante Rocío
Published: May 2018

The Cochabamba Water War (2000) is well renowned for being a part of the civil society versus water service delivery debate. From a situation of service privatization, limited access, and an inexistent institutional framework in 2000, the current situation in the Cochabamba Valley faces increasing water scarcity within a weak institutional set up. To alleviate the situation, the government of Evo Morales has been actively funding projects considering an Integrated Water Resources Management (IWRM) but confronting customary water rights in rural communities and thus increasing the level of conflict between water uses. Amid these two water management practices appears the Agenda del Agua Cochabamba (AdA)—the Cochabamba Water Agenda—claiming water as part of the commons and not a resource. This paper explains the paradigm’s values behind the conflicting IWRM and water rights’ water management practices and analyses the AdA under a governability framework identifying the barriers and drivers for its implementation.
Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening
Authors: Becker Ann-Kristin , Erfle Holger , Gunkel Manuel , Beil Nina , Kaderali Lars , Starkuviene Vytaute
Published: May 2018

Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed in parallel. Each of the formats possesses its own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNA interference (RNAi)-mediated fluorescence microscopy-based assays, namely epidermal growth factor (EGF) internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data with the appearance of the expected phenotypes significantly distinct from the negative controls. The measurements of cell cycle progression were less variable in multi-well plates. The result can largely be attributed to higher cell numbers resulting in less data variability when dealing with the assay generating phenotypic cell subpopulations. The EGF internalization assay with a uniform phenotype over nearly the whole cell population performed better on cell arrays than in multi-well plates. The result was achieved by scoring five times less cells on cell arrays than in multi-well plates, indicating the efficiency of the cell array format. Our data indicate that the choice of the screening platform primarily depends on the type of the cellular assay to achieve a maximum data quality and screen efficiency.
Invasion-Related Factors as Potential Diagnostic and Therapeutic Targets in Oral Squamous Cell Carcinoma—A Review
Authors: Siriwardena Samadarani , Tsunematsu Takaaki , Qi Guangying , Ishimaru Naozumi , Kudo Yasusei
Published: May 2018

It is well recognized that the presence of cervical lymph node metastasis is the most important prognostic factor in oral squamous cell carcinoma (OSCC). In solid epithelial cancer, the first step during the process of metastasis is the invasion of cancer cells into the underlying stroma, breaching the basement membrane (BM)—the natural barrier between epithelium and the underlying extracellular matrix (ECM). The ability to invade and metastasize is a key hallmark of cancer progression, and the most complicated and least understood. These topics continue to be very active fields of cancer research. A number of processes, factors, and signaling pathways are involved in regulating invasion and metastasis. However, appropriate clinical trials for anti-cancer drugs targeting the invasion of OSCC are incomplete. In this review, we summarize the recent progress on invasion-related factors and emerging molecular determinants which can be used as potential for diagnostic and therapeutic targets in OSCC.
Inhibition of Proliferation in U937 Cells Treated by Blue Light Irradiation and Combined Blue Light Irradiation/Drug
Authors: Zhuang Jianjian , Liu Junsong , Gao Xuan , Li Hongdong
Published: May 2018

The cell viability and apoptosis of tumor U937 cells treated by blue light (BL) irradiation have been examined. BL irradiation can specially inhibit the proliferation and promote the apoptosis of U937 cells, relating to the production of reactive oxygen species (ROS) and the decline of mitochondrial membrane potential (ΔΨm). The apoptosis is further associated with varying downregulated B-cell lymphoma-extra large (Bcl-XL) and B-cell lymphoma 2 (Bcl-2) genes, upregulated Bcl-2-associated X (Bax) gene, the activation of caspase-3 and caspase-9, and the cleavage of poly (ADP-ribose) polymerase (PARP) by the BL irradiation process. Moreover, BL irradiation induced proliferation inhibition is higher than that treated by a common chemotherapeutic drug of homoharringtonine (HHT). When we synergize BL irradiation with HHT (BL-HHT), a higher proliferation inhibition is obtained than that treated by BL irradiation or HHT alone. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of acute myeloid leukemia, not limited to U937 cells.
Immunomodulatory Function of Myeloid-Derived Suppressor Cells during B Cell-Mediated Immune Responses
Authors: Özkan Bilgenaz , Lim Heejin , Park Sung-Gyoo
Published: May 2018

Myeloid-derived suppressor cells (MDSCs) play roles in immune regulation during neoplastic and non-neoplastic inflammatory responses. This immune regulatory function is directed mainly toward T cells. However, MDSCs also regulate other cell populations, including B cells, during inflammatory responses. Indeed, B cells are essential for antibody-mediated immune responses. MDSCs regulate B cell immune responses directly via expression of effector molecules and indirectly by controlling other immune regulatory cells. B cell-mediated immune responses are a major component of the overall immune response; thus, MDSCs play a prominent role in their regulation. Here, we review the current knowledge about MDSC-mediated regulation of B cell responses.
Silver Nanoparticles: Two-Faced Neuronal Differentiation-Inducing Material in Neuroblastoma (SH-SY5Y) Cells
Authors: Abdal Dayem Ahmed , Lee Soo , Choi Hye , Cho Ssang-Goo
Published: May 2018

We have previously demonstrated the potential of biologically synthesized silver nanoparticles (AgNP) in the induction of neuronal differentiation of human neuroblastoma, SH-SY5Y cells; we aimed herein to unveil its molecular mechanism in comparison to the well-known neuronal differentiation-inducing agent, all-trans-retinoic acid (RA). AgNP-treated SH-SY5Y cells showed significantly higher reactive oxygen species (ROS) generation, stronger mitochondrial membrane depolarization, lower dual-specificity phosphatase expression, higher extracellular-signal-regulated kinase (ERK) phosphorylation, lower AKT phosphorylation, and lower expression of the genes encoding the antioxidant enzymes than RA-treated cells. Notably, pretreatment with N-acetyl-l-cysteine significantly abolished AgNP-induced neuronal differentiation, but not in that induced by RA. ERK inhibition, but not AKT inhibition, suppresses neurite growth that is induced by AgNP. Taken together, our results uncover the pivotal contribution of ROS in the AgNP-induced neuronal differentiation mechanism, which is different from that of RA. However, the negative consequence of AgNP-induced neurite growth may be high ROS generation and the downregulation of the expression of the genes encoding the antioxidant enzymes, which prompts the future consideration and an in-depth study of the application of AgNP-differentiated cells in neurodegenerative disease therapy.
Fast Identification of High Utility Itemsets from Candidates
Authors: Qu Jun-Feng , Liu Mengchi , Xin Chunsheng , Wu Zhongbo
Published: May 2018

High utility itemsets (HUIs) are sets of items with high utility, like profit, in a database. Efficient mining of high utility itemsets is an important problem in the data mining area. Many mining algorithms adopt a two-phase framework. They first generate a set of candidate itemsets by roughly overestimating the utilities of all itemsets in a database, and subsequently compute the exact utility of each candidate to identify HUIs. Therefore, the major costs in these algorithms come from candidate generation and utility computation. Previous works mainly focus on how to reduce the number of candidates, without dedicating much attention to utility computation, to the best of our knowledge. However, we find that, for a mining task, the time of utility computation in two-phase algorithms dominates the whole running time of these algorithms. Therefore, it is important to optimize utility computation. In this paper, we first give a basic algorithm for HUI identification, the core of which is a utility computation procedure. Subsequently, a novel candidate tree structure is proposed for storing candidate itemsets, and a candidate tree-based algorithm is developed for fast HUI identification, in which there is an efficient utility computation procedure. Extensive experimental results show that the candidate tree-based algorithm outperforms the basic algorithm and the performance of two-phase algorithms, integrating the candidate tree algorithm as their second step, can be significantly improved.
Hardware Efficient Architecture for Element-Based Lattice Reduction Aided K-Best Detector for MIMO Systems
Authors: Halak Basel , El-Hajjar Mohammed , Hassanein Ahmed
Published: May 2018

Multiple-Input Multiple-Output (MIMO) systems are characterised by increased capacity and improved performance compared to the single-input single-output (SISO) systems. One of the main challenge in the design of MIMO systems is the detection of the transmitted signals due to the interference caused by the multiple simultaneously transmitted symbols from the multiple transmit antennas. Several detection techniques have been proposed in the literature in order to reduce the detection complexity, while maintaining the required quality of service. Among these low-complexity techniques is the Lattice Reduction (LR), which can provide good performance and significantly lower complexity compared to Maximum Likelihood (ML) detector. In this paper we propose to use the so-called Element-based Lattice Reduction (ELR) combined with K-Best detector for the sake of attaining a better Bit Error Ratio (BER) performance and lower complexity than the conventional Lenstra, Lanstra, and Lovasz (LLL) LR-aided detection. Additionally, we propose a hardware implementation for the ELR-aided K-Best detector for a MIMO system equipped with four transmit and four receive antennas. The ELR-aided K-Best detector requires an extra 18% increase in power consumption and an extra 20% in area overhead compared to a regular K-Best detector dispensing with ELR, where this increase in the hardware requirements is needed in order to achieve a 2 dB performance improvement at a bit error rate of 10−5.
The Evaluation of Damage Effects on MgO Added Concrete with Slag Cement Exposed to Calcium Chloride Deicing Salt
Authors: Jang Jae-Kyeong , Kim Hong-Gi , Kim Jun-Hyeong , Ryou Jae-Suk
Published: May 2018

Concrete systems exposed to deicers are damaged in physical and chemical ways. In mitigating the damage from CaCl2 deicers, the usage of ground slag cement and MgO are investigated. Ordinary Portland cement (OPC) and slag cement are used in different proportions as the binding material, and MgO in doses of 0%, 5%, 7%, and 10% are added to the systems. After 28 days of water-curing, the specimens are immersed in 30% CaCl2 solution by mass for 180 days. Compressive strength test, carbonation test, chloride penetration test, chloride content test, XRD analysis, and SEM-EDAX analysis are conducted to evaluate the damage effects of the deicing solution. Up to 28 days, plain specimens with increasing MgO show a decrease in compressive strength, an increase in carbonation resistance, and a decrease in chloride penetration resistance, whereas the S30- and S50- specimens show a slight increase in compressive strength, an increase in carbonation resistance, and a slight increase in chloride penetration resistance. After 180 days of immersion in deicing solution, specimens with MgO retain their compressive strength longer and show improved durability. Furthermore, the addition of MgO to concrete systems with slag cement induces the formation of magnesium silicate hydrate (M-S-H) phases.
External-Stimuli-Assisted Control over Assemblies of Plasmonic Metals
Authors: Watanabe Kanako , Kuroda Kotaro , Nagao Daisuke
Published: May 2018

Assembly of plasmonic nanoparticles (NPs) in suspensions is a promising approach for the control of optical and sensing properties that depend on the assembled states of plasmonic NPs. This review focuses on the controlling methods to assemble the NP via external stimuli such as pH, temperature, light, magnetic field, and electric field. External stimuli are introduced as powerful tools to assemble the NPs because of various operational factors, such as the intensity, application time, and frequency, which can be employed. In addition to a summary of recent studies on the controlling methods, a future study on the reversible control over assembled states of the plasmonic NPs via external stimuli is proposed.
Nanocomposites Based on Biodegradable Polymers
Authors: Armentano Ilaria , Puglia Debora , Luzi Francesca , Arciola Carla , Morena Francesco , Martino Sabata , Torre Luigi
Published: May 2018

In the present review paper, our main results on nanocomposites based on biodegradable polymers (on a time scale from 2010 to 2018) are reported. We mainly focused our attention on commercial biodegradable polymers, which we mixed with different nanofillers and/or additives with the final aim of developing new materials with tunable specific properties. A wide list of nanofillers have been considered according to their shape, properties, and functionalization routes, and the results have been discussed looking at their roles on the basis of different adopted processing routes (solvent-based or melt-mixing processes). Two main application fields of nanocomposite based on biodegradable polymers have been considered: the specific interaction with stem cells in the regenerative medicine applications or as antimicrobial materials and the active role of selected nanofillers in food packaging applications have been critically revised, with the main aim of providing an overview of the authors’ contribution to the state of the art in the field of biodegradable polymeric nanocomposites.
Electronic, Magnetic, Half-Metallic, and Mechanical Properties of a New Equiatomic Quaternary Heusler Compound YRhTiGe: A First-Principles Study
Authors: Han Yilin , Wu Yang , Li Tingzhou , Khenata R. , Yang Tie , Wang Xiaotian
Published: May 2018

We apply First-principles theory to study the electronic structure as well as the magnetic and mechanical characteristics of YRhTiGe, a newly-designed Y-based quaternary equiatomic Heusler compound. This compound is half-metallic in nature with a ferromagnetic ground state. The total magnetic moment of YRhTiGe is 2 μB and it obeys the Slater-Pauling rule, Mt = Zt − 18, where Mt and Zt are the total magnetic moment and total number of valence electrons, respectively. The magnetic and half-metallic behaviors at its equilibrium and strained lattice constants have been discussed in detail. In addition, for FM-type YRhTiGe, its polycrystalline mechanical features such as Poisson’s ratio, Lame constants, Kleinman parameter and hardness, are also computed according to the well-known Voigt-Reuss-Hill approximation. We investigate the mechanical anisotropy of YRhTiGe using the directional dependences of the Young’s modulus and the shear modulus. Finally, we prove this compound is structurally and mechanically stable. This theoretical investigation provides further insight into the application of Y-based compounds as spintronic materials.
Comparative Physicochemical and Electrochemical Characterization of the Structure and Composition of Thin Pd Binary and Ternary Codeposits with Pt, Ru, and Rh
Authors: Hubkowska Katarzyna , Łukaszewski Mariusz , Soszko Michał , Koss Urszula , Hamankiewicz Bartosz , Czerwiński Andrzej
Published: May 2018

Pd-Ru, Pd-Rh, Pd-Pt-Ru, and Pd-Rh-Ru electrodes were prepared as thin layers by potentiostatic codeposition or chemical co-precipitation of metals from baths containing mixtures of chloride salts. The formation of substitutional solid solutions, with lattice parameters smaller than that of pure Pd, was confirmed by X-ray diffraction (XRD). The compositions at various levels of sample volume and thickness were analyzed by inductively coupled plasma atomic emission spectroscopy (ICP-AES), energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), Auger electron spectroscopy (AES), and also electrochemically by cyclic voltammetry (CV) in 0.5 M H2SO4. The differences between surface, subsurface, and bulk compositions were compared for various systems in a wide composition spectrum.
The Role of 18-Fluoro-2-Deoxy-Glucose Positron Emission Tomography/Computed Tomography as Response and Prognosis Predictive Factor of Concurrent Chemoradiotherapy after Induction Chemotherapy in Head and Neck Squamous Cell Carcinoma: A Prospective Study
Authors: Šedienė Severina , Kulakienė Ilona , Rudžianskas Viktoras , Ambrazienė Rita
Published: May 2018

Background and objectives: The importance of induction chemotherapy (ICT) followed by concurrent chemoradiotherapy (CCRT) has been re-established in recent years aiming at fewer metastatic sites and better control of the disease. We prospectively studied the possibility of early prediction of overall survival (OS) and progression-free survival (PFS) after 3 cycles of chemotherapy with doxetacel, cisplatin and 5-fluorouracil using 18-fluoro-2-deoxy-glucose positron emission tomography computed tomography (18F-FDG PET/CT) in patients with head and neck squamous cell cancer. To our knowledge, this is the first such study. Materials and Methods: Thirty-five patients were studied. They underwent an 18F-FDG PET/CT examination twice: a day before ICT and 10–14 days after the last cycle of ICT. Tumor-standardized uptake value (SUVmax) and hypermetabolic tumor volume were measured on both scans. The mean age of patients was 56.5 years. Complete responses to CCRT PFS and OS were calculated. Results: Our results showed that a decrease of ≥30% in the SUVmax value after ICT was a prognostic factor of tumor response to PFS and OS (p = 0.026 and p = 0.021). The groups of patients with a SUVmax between 10 and 14.5 in the primary tumor on a pre-ICT 18F-FDG PET/CT scan had statistically shorter PFS and OS (p = 0.001, p = 0.006) when compared with other groups of patients with SUVmax less than 10 or SUVmax more than 14.5. A decrease of less than 55% of hypermetabolic tumor volume of the primary tumor was significantly related to poor prognosis in PFS and OS (p = 0.033, p = 0.017). Conclusions: SUVmax and hypermetabolic tumor volume measured on 18F-FDG PET/CT after ICT might be valuable prognostic tools for predicting OS and PFS and, thus, for the selection of patients with head and neck cancer who will benefit from CCRT.
Editorial for the Special Issue on Piezoelectric MEMS
Authors: Schmid Ulrich , Schneider Michael
Published: May 2018

Electromechanical transducers that utilize the piezoelectric effect have been increasingly used in micro-electromechanical systems (MEMS) either as substrates or as thin films[...]
Fabrication and Characterization of Inhomogeneous Curved Artificial Compound Eye
Authors: Liu Fengli , Diao Xiaolei , Li Lun , Hao Yongping , Jiao Zhongyuan
Published: May 2018

Compared with the conventional compound eye processing method, a new fabrication method—namely, a mold casting method—was presented. This method is simple, low-cost, easy to implement, and can be reused. A bionic compound eye array model with 61 ommatidia arranged inhomogeneously onto a curved surface was fabricated. The curved surface had a radius of 9 mm and a thickness of 0.5 mm. The margin imaging quality was improved significantly by the analysis of light beam focus and the optical imaging properties of the fabricated compound eye. The sub-image of each ommatidium had a high resolution. There was 5% error between the collecting spot brightness and simulation analysis results, which proved that the production method is feasible.
Effects of Microbial Growth Conditions on Synthesis of Magnetite Nanoparticles using Indigenous Fe(III)-Reducing Bacteria
Authors: Kim Yumi , Roh Yul
Published: May 2018

Recent researches have shown that microbe–metal interactions play an important role in metal cycling and biomineralization in subsurface environments. The objective of this research was to study the effects of microbial growth conditions for size control on the synthesis of magnetite nanoparticles using Fe(III)-reducing bacteria enriched from intertidal flat sediments in Korea. The microbial formation of the magnetite nanoparticles was examined under various incubation temperatures (8–35 °C), concentrations (20–60 mM) of magnetite precursor, medium pHs (6.5–8.5), and incubation times (0–3 weeks). The Fe(III)-reducing bacteria formed 2~10 nm-sized magnetite (Fe3O4) by reduction of 40 mM akaganeite, especially under the conditions at 25 °C and medium pH = 8.5 within a 1-week incubation time. The magnetite nanoparticles formed by microbial processes exhibited superparamagnetic behavior.
Tetrel Bonds with π-Electrons Acting as Lewis Bases—Theoretical Results and Experimental Evidences
Authors: Grabowski Sławomir
Published: May 2018

MP2/aug-cc-pVTZ calculations were carried out for the ZFH3-B complexes (Z = C, Si, Ge, Sn and Pb; B = C2H2, C2H4, C6H6 and C5H5-; relativistic effects were taken into account for Ge, Sn and Pb elements). These calculations are supported by other approaches; the decomposition of the energy of interaction, Quantum Theory of Atoms in Molecules (QTAIM) and Natural Bond Orbital (NBO) method. The results show that tetrel bonds with π-electrons as Lewis bases are classified as Z···C links between single centers (C is an atom of the π-electron system) or as Z···π interactions where F‒Z bond is directed to the mid-point (or nearly so) of the CC bond of the Lewis base. The analogous systems with Z···C/π interactions were found in the Cambridge Structural Database (CSD). It was found that the strength of interaction increases with the increase of the atomic number of the tetrel element and that for heavier tetrel elements the ZFH3 tetrahedral structure is more deformed towards the structure with the planar ZH3 fragment. The results of calculations show that the tetrel bond is sometimes accompanied by the Z-H···C hydrogen bond or even sometimes the ZFH3-B complexes are linked only by the hydrogen bond interaction.
Study on the Polar Extracts of Dendrobium nobile, D. officinale, D. loddigesii, and Flickingeria fimbriata: Metabolite Identification, Content Evaluation, and Bioactivity Assay
Authors: Chen Huiping , Li Xuewen , Xu Yongli , Lo Kakei , Zheng Huizhen , Hu Haiyan , Wang Jun , Lin Yongcheng
Published: May 2018

The polar extract of the Dendrobium species or F. fimbriata (a substitute of Dendrobium), between the fat-soluble extract and polysaccharide has barely been researched. This report worked on the qualitative and quantitative studies of polar extracts from D. nobile, D. officinale, D. loddigesii, and F. fimbriata. Eight water-soluble metabolites containing a new diglucoside, flifimdioside A (1), and a rare imidazolium-type alkaloid, anosmine (4), were identified using chromatography as well as spectroscopic techniques. Their contents in the four herbs were high, approximately 0.9–3.7 mg/g based on the analysis of quantitative nuclear magnetic resonance (qNMR) spectroscopy. Biological activity evaluation showed that the polar extract of F. fimbriata or its pure component had good antioxidant and neuroprotective activity; compounds 1‒4 and shihunine (8) showed weak α-glucosidase inhibitory activity; 4 and 8 had weak anti-inflammatory activity. Under trial conditions, all samples had no cytotoxic activity.
CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies
Authors: Banerjee Tirtha , Monteiro Antónia
Published: May 2018

CRISPR-Cas9 is revolutionizing the field of genome editing in non-model organisms. The robustness, ease of use, replicability and affordability of the technology has resulted in its widespread adoption among researchers. The African butterfly Bicyclus anynana is an emerging model lepidopteran species in the field of evo-devo, with a sequenced genome and amenable to germ line transformation. However, efficient genome editing tools to accelerate the pace of functional genetic research in this species have only recently become available with CRISPR-Cas9 technology. Here, we provide a detailed explanation of the CRISPR-Cas9 protocol we follow in the lab. The technique has been successfully implemented to knock-out genes associated with eyespot development and melanin pigmentation.
Highly Efficient, Low-Cost, and Magnetically Recoverable FePt–Ag Nanocatalysts: Towards Green Reduction of Organic Dyes
Authors: Liu Yang , Zhang Yuanyuan , Kou Qiangwei , Chen Yue , Sun Yantao , Han Donglai , Wang Dandan , Lu Ziyang , Chen Lei , Yang Jinghai , Xing Scott
Published: May 2018

Nowadays, synthetic organic dyes and pigments discharged from numerous industries are causing unprecedentedly severe water environmental pollution, and conventional water treatment processes are hindered due to the corresponding sophisticated aromatic structures, hydrophilic nature, and high stability against light, temperature, etc. Herein, we report an efficient fabrication strategy to develop a new type of highly efficient, low-cost, and magnetically recoverable nanocatalyst, i.e., FePt–Ag nanocomposites, for the reduction of methyl orange (MO) and rhodamine B (RhB), by a facile seed deposition process. X-ray diffraction results elaborate that the as-synthesized FePt–Ag nanocomposites are pure disordered face-centered cubic phase. Transmission electron microscopy studies demonstrate that the amount of Ag seeds deposited onto the surfaces of FePt nanocrystals increases when increasing the additive amount of silver colloids. The linear correlation of the MO and RhB concentration versus reaction time catalyzed by FePt–Ag nanocatalysts is in line with pseudo-first-order kinetics. The reduction rate constants of MO and RhB increase with the increase of the amount of Ag seeds. FePt–Ag nanocomposites show good separation ability and reusability, and could be repeatedly applied for nearly complete reduction of MO and RhB for at least six successive cycles. Such cost-effective and recyclable nanocatalysts provide a new material family for use in environmental protection applications.
Diet-Induced Weight Loss Has No Effect on Psychological Stress in Overweight and Obese Adults: A Meta-Analysis of Randomized Controlled Trials
Authors: Booth Alison , Wang Xiaodan , Turner Anne , Nowson Caryl , Torres Susan
Published: May 2018

The effect of weight loss on psychological stress is unknown. The study aimed to investigate the effect of diet-induced weight loss in overweight and obese adults on psychological measures of stress through a meta-analysis of randomized controlled trials (RCTs). Databases including Medline Complete, Embase and PsycINFO were searched up to February 2018 for diet-induced weight loss RCTs, which included self-reported assessment of psychological stress. The mean difference between the intervention and control group of changes in stress (intervention—baseline) was used. Ten RCTs were included with 615 participants (502 women, age range 20–80 years). Overall, there was no change in stress (mean difference −0.06, 95% CI: −0.17, 0.06, p = 0.33) and no change in the five studies with a significant reduction in weight in the intervention group compared to a control group that lost no weight (mean difference in weight −3.9 Kg, 95% CI: −5.51, −2.29, p < 0.0001; mean difference in stress 0.04, 95% CI: −0.17, 0.25, p = 0.71). For all analyses, there was low heterogeneity. The benefits of weight loss for those who are overweight and obese do not appear to either increase or reduce psychological stress at the end of the weight loss period.
Cost-Effectiveness of Product Reformulation in Response to the Health Star Rating Food Labelling System in Australia
Authors: Mantilla Herrera Ana , Crino Michelle , Erskine Holly , Sacks Gary , Ananthapavan Jaithri , Mhurchu Cliona , Lee Yong
Published: May 2018

The Health Star Rating (HSR) system is a voluntary front-of-pack labelling (FoPL) initiative endorsed by the Australian government in 2014. This study examines the impact of the HSR system on pre-packaged food reformulation measured by changes in energy density between products with and without HSR. The cost-effectiveness of the HSR system was modelled using a proportional multi-state life table Markov model for the 2010 Australian population. We evaluated scenarios in which the HSR system was implemented on a voluntary and mandatory basis (i.e., HSR uptake across 6.7% and 100% of applicable products, respectively). The main outcomes were health-adjusted life years (HALYs), net costs, and incremental cost-effectiveness ratios (ICERs). These were calculated with accompanying 95% uncertainty intervals (95% UI). The model predicted that HSR-attributable reformulation leads to small changes in mean population energy intake (voluntary: −0.98 kJ/day; mandatory: −11.81 kJ/day). These are likely to result in changes in mean body weight (voluntary: −0.01 kg [95% UI: −0.012 to −0.006]; mandatory: −0.11 kg [95% UI: −0.14 to −0.07]), and HALYs gained (voluntary: 4207 HALYs gained [95% UI: 2438 to 6081]; mandatory: 49,949 HALYs gained [95% UI: 29,291 to 72,153]). The HSR system could be considered cost-effective relative to a willingness-to-pay threshold of A$50,000 per HALY (incremental cost effectiveness ratio for voluntary: A$1728 per HALY [95% UI: dominant to 10,445] and mandatory: A$4752 per HALY [95% UI: dominant to 16,236]).
Weaning Time in Preterm Infants: An Audit of Italian Primary Care Paediatricians
Authors: Baldassarre Maria , Di Mauro Antonio , Pedico Annarita , Rizzo Valentina , Capozza Manuela , Meneghin Fabio , Lista Gianluca , Laforgia Nicola ,   On behalf of Italian Society of Pediatrics (SIP), Italian Society of Neonatology (SIN), Italian Society of Pediatric Gastroenterology, Hepatology, and Nutrition (SIGENP) and Italian Federation of Paediatricians (FIMP)
Published: May 2018

Introduction: According to the 2016 Italian National Institute of Statistics (Istat) data in Italy, about 6.7% of all newborns are born prematurely. Due to the lack of data on current complementary feeding in preterm infants in Italy, the aim of the survey was to evaluate individual attitudes of primary care paediatricians, concerning the introduction of complementary foods in preterm infants. Methods: An internet-based survey was conducted among primary care paediatricians, working in Italy, regarding (1) timing of the introduction of complementary foods to preterm newborns; (2) type of complementary foods introduced; (3) vitamin D and iron supplementations. Results: A total of 347 primary care Italian paediatricians answered the questionnaire; 44% of responders based the timing of the introduction of solid food exclusively on an infant’s age, 18% on an infant’s neurodevelopmental status and 4% on the body weight; the remaining 34% based the timing on two or more of these aspects. The type of complementary foods did not comply with an evidence-based sequence; 98% of participants promoted vitamin D supplementation and 89% promoted iron supplementation with great diversity in timing and doses. Conclusions: Due to limited evidence, there is a great heterogeneity in the attitudes of primary care paediatricians concerning the introduction of complementary foods to preterm newborns. Further research is needed to provide evidence-based guidelines regarding weaning preterm newborns.
Buckwheat and CVD Risk Markers: A Systematic Review and Meta-Analysis
Authors: Li Liangkui , Lietz Georg , Seal Chris
Published: May 2018

The effects of buckwheat intake on cardiovascular diseases (CVDs) have not been systematically investigated. The aim of the present study was to comprehensively summarize studies in humans and animals, evaluating the impact of buckwheat consumption on CVD risk markers and to conduct a meta-analysis of relevant data. Thirteen randomized, controlled human studies, two cross-sectional human studies and twenty-one animal studies were identified. Using random-effects models, the weighted mean difference of post-intervention concentrations of blood glucose, total cholesterol and triglycerides were significantly decreased following buckwheat intervention compared with controls [differences in blood glucose: −0.85 mmol/L (95% CI: −1.31, −0.39), total cholesterol: 0.50 mmol/L (95% CI: −0.80, −0.20) and triglycerides: 0.25 mmol/L (95% CI: −0.49, −0.02)]. Responses of a similar magnitude were seen in two cross-sectional studies. For animal studies, nineteen of twenty-one studies showed a significant reduction in total cholesterol of between 12% and 54%, and fourteen of twenty studies showed a significant reduction in triglycerides of between 2% and 74%. All exhibited high unexplained heterogeneity. There was inconsistency in HDL cholesterol outcomes in both human and animal studies. It remains unclear whether increased buckwheat intake significantly benefits other markers of CVD risk, such as weight, blood pressure, insulin, and LDL-cholesterol, and underlying mechanisms responsible for any effects are unclear.
The Potential Cost-Effectiveness and Equity Impacts of Restricting Television Advertising of Unhealthy Food and Beverages to Australian Children
Authors: Brown Vicki , Ananthapavan Jaithri , Veerman Lennert , Sacks Gary , Lal Anita , Peeters Anna , Backholer Kathryn , Moodie Marjory
Published: May 2018

Television (TV) advertising of food and beverages high in fat, sugar and salt (HFSS) influences food preferences and consumption. Children from lower socioeconomic position (SEP) have higher exposure to TV advertising due to more time spent watching TV. This paper sought to estimate the cost-effectiveness of legislation to restrict HFSS TV advertising until 9:30 pm, and to examine how health benefits and healthcare cost-savings differ by SEP. Cost-effectiveness modelling was undertaken (i) at the population level, and (ii) by area-level SEP. A multi-state multiple-cohort lifetable model was used to estimate obesity-related health outcomes and healthcare cost-savings over the lifetime of the 2010 Australian population. Incremental cost-effectiveness ratios (ICERs) were reported, with assumptions tested through sensitivity analyses. An intervention restricting HFSS TV advertising would cost AUD5.9M (95% UI AUD5.8M–AUD7M), resulting in modelled reductions in energy intake (mean 115 kJ/day) and body mass index (BMI) (mean 0.352 kg/m2). The intervention is likely to be cost-saving, with 1.4 times higher total cost-savings and 1.5 times higher health benefits in the most disadvantaged socioeconomic group (17,512 HALYs saved (95% UI 10,372–25,155); total cost-savings AUD126.3M (95% UI AUD58.7M–196.9M) over the lifetime) compared to the least disadvantaged socioeconomic group (11,321 HALYs saved (95% UI 6812–15,679); total cost-savings AUD90.9M (95% UI AUD44.3M–136.3M)). Legislation to restrict HFSS TV advertising is likely to be cost-effective, with greater health benefits and healthcare cost-savings for children with low SEP.
The Effects of Dabigatran and Rivaroxaban on Markers of Polymorphonuclear Leukocyte Activation
Authors: Richards Guy , Theron Annette , Tintinger Gregory , Anderson Ronald
Published: May 2018

Dabigatran is an oral direct thrombin inhibitor, and rivaroxaban, a factor Xa inhibitor. Dabigatran has been implicated in the etiology of acute coronary syndromes and as these occur following inflammatory changes in the endothelium, we investigated the inflammatory potential of these agents in vitro. In order to do so, polymorphonuclear leukocytes (PMNL) were isolated from heparinized venous blood from non-smoking, healthy adults and exposed to dabigatran or rivaroxaban (0.5–10 µM). Generation of reactive oxygen species (ROS), elastase release, cytosolic Ca2+ fluxes, neutrophil extracellular trap (NET) formation and cell viability were measured using chemiluminescence, spectrophotometric and flow cytometric procedures respectively. However, with the exception of modest inhibitory effects on elastase release, neither agent at concentrations of up to 10 µM affected these markers of PMNL activation. Although no pro-inflammatory effects of dabigatran nor any difference between the two test agents were detected in vitro, the existence of a pro-inflammatory mechanism involving the generation of thrombin during dabigatran therapy cannot be fully excluded.
Self-Organization and Swelling of Ruthenium-Metal Coordination Polymers with PTA (Metal = Ag, Au, Co)
Authors: Sierra-Martin Benjamin , Serrano-Ruiz Manuel , García-Sakai Victoria , Scalambra Franco , Romerosa Antonio , Fernandez-Barbero Antonio
Published: May 2018

We present the internal structure and dynamics of novel coordination polymers based on two metal-containing moieties Ru-X (X: Ag, Au, Co), bridged through the phosphine PTA (3,5,7-triaza-phosphaadamantane). X-ray scattering gives the heterometallic polymer organization. Quasi-elastic neutron scattering measurements over a broad temperature range show a transition from vibrational Debye-Waller behavior to a more dynamically active state, but with rather localized motions, coinciding with the loss of structural water at around room temperature. Light scattering reveals that the polymers self-associate to form stable micro-particles in aqueous solution with a thermally driven volume transition. This is described by the Flory theory for polymers in solution, in which the polymer solvency is calculated as a function of the temperature. Polymer self-organization is further studied by small-angle neutron scattering and electron microscopy. A polymer parallel-plane model with gaps controlled by the environmental temperature is proposed.
Synthesis of Quaternary Ammonium Salts of Chitosan Bearing Halogenated Acetate for Antifungal and Antibacterial Activities
Authors: Zhang Jingjing , Tan Wenqiang , Luan Fang , Yin Xiuli , Dong Fang , Li Qing , Guo Zhanyong
Published: May 2018

In this paper, quaternary ammonium salts of chitosan bearing halogenated acetate, including N,N,N-trimethyl chitosan chloroacetate (TMCSC), N,N,N-trimethyl chitosan dichloroacetate (TMCSDC), N,N,N-trimethyl chitosan trichloroacetate (TMCSTC), and N,N,N-trimethyl chitosan trifluoroacetate (TMCSTF), were prepared via N,N,N-trimethyl chitosan iodide (TMCSI). The obtained chitosan derivatives were characterized by FT-IR, 1H NMR spectra, 13C NMR spectra, and elemental analysis. Their antifungal property against Fusarium oxysporum f. sp. cucumebrium Owen (F. oxysporum f. sp. cucumebrium Owen), Botrytis cinerea (B. cinerea), and Phomopsis asparagi (P. asparagi) were evaluated by hyphal measurement method at concentrations ranging from 0.08 mg/mL to 0.8 mg/mL. Meanwhile, two common bacteria, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), were selected as the model Gram-negative and Gram-positive bacteria to evaluate the antibacterial property of the chitosan derivatives by agar well diffusion method. The results showed that TMCSC, TMCSDC, TMCSTC, and TMCSTF had better antifungal and antibacterial activities than chitosan and TMCSI. In particular, a rule showed that the inhibitory activity decreased in the order: TMCSTF > TMCSTC > TMCSDC > TMCSC > TMCSI > chitosan, which was consistent with the electron-withdrawing property of different halogenated acetate. Apparently, the quaternary ammonium salts of chitosan with stronger electron withdrawing ability possessed relatively greater antifungal and antibacterial activities. This experiment provides a potentially feasible method for the further utilization of chitosan in fields of antifungal and antibacterial biomaterials.
Proteomics of Human Retinal Pigment Epithelium (RPE) Cells
Authors: Beranova-Giorgianni Sarka , Giorgianni Francesco
Published: May 2018

Retinal pigment epithelium (RPE) are specialized, multifunctional cells in the retina that form a monolayer of cuboidal, polarized cells adjoining the photoreceptor cells. The RPE are a critical component of the blood-retinal barrier, and they play essential functional roles for maintenance of retinal homeostasis and for support and health of photoreceptors. Age-dependent, progressive dysfunction and death of RPE cells and the resultant loss of photoreceptors contribute significantly to the development and progression of age-related macular degeneration (AMD) and other retinal degenerative diseases. Several different RPE cell culture models have been developed and utilized extensively as surrogates for cellular and molecular examinations of the RPE, and a large body of knowledge on RPE function in normal and pathological scenarios has been amassed in studies with cultured RPE. Proteomics has been an integral part of research efforts aimed to advance our understanding of RPE cell biology in health and disease. This review focuses on applications of proteomics to in vitro qualitative and quantitative investigation of human RPE cell culture models. The disease context discussed focuses on AMD.