bluuebunny/crossref_metadata_2025 huggingface dataset:
Predation, but not herbivory, declines with elevation in a tropical rainforest
Authors: Barlow B. E. L. , Nakamura A. , Ashton L. A.
Published: May 2024

Naturally, insect herbivore populations are controlled by their plant hosts and predators. These ‘bottom-up’ and ‘top-down’ controls influence leaf area lost to herbivory. Bottom-up control of herbivory may be driven by leaf nutrients and plant defences. Top-down control can be driven by abundance and species richness of natural enemies, host or prey specificity, and predation strategies (e.g., active searching or sit-and-wait ‘ambush’ predation). The relative importance of bottom-up and top-down controls is unresolved but likely to vary spatially and temporally and under different environmental conditions such as changing temperature. We surveyed leaf carbon and nitrogen, leaf area loss, and attacks on plasticine caterpillars across a tropical elevational gradient in Xishuangbanna, Yunnan Provence, China. We show that predatory foraging activity decreases with elevation and temperature, whereas leaf nutrients and leaf area loss from herbivory remains more or less constant. Predation patterns were driven by ants, which are thermophiles and therefore more active, abundant, and diverse at warmer, lower elevations. Leaf nutritional values are important in driving herbivory patterns as herbivory was stable across this gradient, but other factors such as mechanical defences and herbivore-induced plant volatiles demand further study. Elevational studies provide insight into how ecosystem function will shift under climate change. As increasing temperatures following climate change allows predatory groups like ants to exploit higher elevations, top-down control in high elevation habitats could increase, resulting in re-wiring of these ecologically sensitive communities. At the same time, top-down control at lower elevations may be at risk if critical thermal maxima for natural enemies are exceeded.
Harnessing the power of MXenes: a comprehensive exploration of their photocatalytic potential in mitigating hazardous dyes and CO2 reduction
Authors: Gulzar Ayesha , Haleem Abdul , Rehman Touseef Ur , Ullah Mohib , Shah Afzal , Ullah Ihsan
Published: May 2024

This comprehensive review extensively explores the potential applications of MXenes as versatile materials in the realm of photocatalysis, with a specific focus on their efficacy in mitigating hazardous dyes and CO2 to less harmful and friendly by-products. The review systematically investigates the unique properties that render MXenes well-suited for photocatalytic purposes and provides a thorough examination of their current state of research. It meticulously summarizes the successes and breakthroughs achieved thus far, offering insights into the advancements that have propelled these materials into the spotlight of photocatalytic research. In addition to highlighting achievements, the review critically addresses the challenges and hurdles that impede the full realization of the potential inherent in MXenes. Here, we have also highlighted the stability problem of MXenes and how to overcome this problem for efficient photocatalysis. The mechanism of photocatalysis was also the main theme of this review article and how to overcome the recombination of photogenerated charges. By identifying these challenges, the review serves as a valuable resource for researchers, providing a roadmap for future endeavours to unlock the untapped capabilities of these materials. It serves as a beacon for environmental researchers, offering valuable insights into the pivotal role these materials can play in creating a more environmentally friendly and safe world. Ultimately, this review contributes significantly to the collective knowledge base and will prove instrumental for researchers and professionals dedicated to environmental protection and sustainable living.

Graphical Abstract
Development of a regulator for high-pressure hydrogen charging and storage system
Authors: Lee Jong Suk , Kim Sa Ryang , Shin Joon , Yun Kyeong Soo , Kim Chang Hoon , Je Gwang Jun , Choi Hyoung Il , Chen Liang
Published: May 2024

Hydrogen emerges as a promising alternative energy source, particularly in fuel cell applications, necessitating efficient and safe charging and storage systems. This paper presents the design and development of a specialized regulator tailored for high-pressure hydrogen environments. Focusing on precision control, the regulator ensures optimal performance and safety during charging and storage phases. In order to improve the unstable response characteristics of the existing commercial hydrogen regulator, which has the same differential pressure generation site and pressure control site, a regulator operating at an ultra-high pressure of 105 MPa was designed by separating the differential pressure site and the control site. And structural safety and performance of the regulator were predicted through structural and flow analysis. The results of structural and flow analysis of a 105 MPa high-pressure regulator that can be used for high-pressure hydrogen charging and storage systems were compared with the experimentally measured values.
Bayesian versus Frequentist approaches in Psychometrics: a bibliometric analysis
Authors: Zagaria Andrea , Lombardi Luigi
Published: May 2024

The increasing popularity of the Bayesian approach in Psychology has prompted metascientific efforts to quantify its prevalence. However, despite enduring debates between proponents of Frequentist and Bayesian schools of thought, no systematic comparison of their prominence has been conducted in existing literature. This brief report fills this gap, examining Bayesian and Frequentist trends in the period from 1964 to 2023 through a meticulous search in PsycINFO. The findings reveal that the Frequentist approach has consistently been more popular than the Bayesian approach in the realm of Psychometrics and Statistical Psychology. However, Bayesian contributions steadily increased from the 80’s onward and appear to be almost as important or even surpassing the Frequentist counterparts in the latest years investigated (2019–2023). Although this observation applies primarily to specialized literature rather than the entire domain of Psychology, it underscores the growing prevalence of the Bayesian approach, signaling attention among specialists in the field.
A spatial perspective on the impact of digital financial inclusion on the employment structure of the labour force: Evidence from Chinese cities
Authors: Hu Junjie , Lu Yang
Published: May 2024

In the context of big data, the rise of digital inclusive finance is driving the digital transformation of various industries, which in turn, has a profound impact on labour employment. Based on the panel data of 258 prefecture-level cities in China from 2011 to 2021, this article evaluates the impact of digital financial inclusion on the employment structure of the labour force and investigates its spatial spillover effects by constructing a spatial Durbin model and a mediation model. The study finds that: (1) Digital financial inclusion increases the employment demand for high-skilled and low-skilled labour and decreases the employment demand for middle-skilled labour, that is, the employment structure of the labour force shows a polarisation trend. (2) There is a positive spatial spillover effect of the impact of the development of digital financial inclusion on the demand for high- and low-skilled labour. (3) Financial digitisation plays an intermediary role in the process of digital financial inclusion affecting the employment structure of the labour force. Taking advantage of the dividends brought about by the development of digital inclusive finance and formulating policies according to the actual situation of each region indicate an undeniable choice for achieving full employment.
A Priest, a Ranch, and los Muchachos: A Study of Race and Clerical Abuse from New Mexico
Authors: Holscher Kathleen
Published: May 2024

Father Ed Donelan came to New Mexico from Massachusetts. The priest worked as a chaplain at a facility for “juvenile delinquents,” and later ran a home for boys he called the Hacienda de los Muchachos. Donelan sexually abused youth at both facilities. This essay considers how Donelan leveraged New Mexico’s juvenile justice and habilitation systems, and racial inequities baked into them, to abuse young people. Within those systems, a Catholic culture of clericalism granted Donelan unlimited access to youth, and enabled him to move unchecked between spiritual and quasi-parental forms of authority. Donelan’s story shows that clericalism is not a one-size-fits-all problem; it manifests differently in relation to different communities. Here clericalism intersects with place-based power structures of race and colonialism to damage in locally specific ways. Donelan’s case demonstrates that scholars who study clerical sexual abuse need to pay attention not only to priests, but also to church and state institutions that rendered certain populations of children especially vulnerable to their bad actions.
Time and title deeds in post-reform agrarian Kenya
Authors: Haugerud Angelique , Mwangi Caroline P.
Published: May 2024

The ‘title deed fix’ – resurgent globally since the 1990s – is part of a wave of market-led agrarian reforms whose outcomes have been mixed. Kenya was the first African country to experiment – starting six decades ago and continuing today – with state-mandated formal land registration and private titling. Today it is among a handful to begin a transition to a digitized land registry. Behind both paper and electronic land documents, however, is a persistent temporal fiction that undergirds state-backed title registries – namely, a constructed present that is out of sync with intersecting biographical and structural temporalities, and that can efface socially recognized pasts, commitments or testimonials. We analyse some consequences of those temporal dissonances, unstable rights durations, and an ensuing limbo that can last decades, through family land stories shared with us during long-term ethnographic research in Kenya’s fertile central highlands. Especially vulnerable to temporal erasure and dispossession when title deed limbo spans decades are divorced or single women and their children, particularly as farmland and non-agricultural employment become more scarce and land markets overheat. Multitemporal family narratives powerfully illustrate why title deeds of any age are best taken as provisional truths rather than legal certainties, and why tenure security is an unstable and reversible process rather than a present or absent condition.
The jet set: Modern RP and the (re)creation of social distinction
Authors: Holmes-Elliott Sophie , Levon Erez
Published: May 2024

While the loss of regional distinctiveness across the southeastern UK is well studied and largely undisputed, there is less consensus about class-based divisions. This paper investigates this question through an updated analysis of the variety emblematic of Britain’s upper class: Received Pronunciation (RP). While previous studies have suggested levelling in RP to a broader standard southeastern norm, our findings indicate that the most recent advances in the variety show it (re)differentiating itself from other varieties in the region. Investigating both individual vowel movements and broader system-wide properties, we argue that the changes observed in RP today result from speakers adopting a particular articulatory setting (lax voice), which has subsequent ramifications on vowel realizations. We suggest that speakers make strategic use of this articulatory setting as a way of embodying an elite persona in the British context, an interpretation that resonates with the social distributions of similar changes in other varieties.
Sticking With It
Authors: Rost Detlef H. , Greiff Samuel , Ladkani Sahar , Stadler Matthias
Published: May 2024

Abstract: The allegedly new volitional-motivational personality construct “grit” is seen as both theoretically and empirically relevant for a host of educational and life outcomes. In a recent debate, however, it has been argued that the construct should be considered as the amalgam of its two assumed subdimensions of Perseverance of Effort (PE) and Consistency of Interest (CI). Opponents of this argument note that the distinction between the two dimensions may merely be the result of different wording (positive and negative) in associated questionnaires. We systematically investigated this notion based on a sample of N = 881 high school students. Participants responded to one of four versions of the short grit scale (Grit-S) asking about their grit in math, foreign language English, and history. The items for PE and CI were each worded either positively or negatively, with all combinations realized. Our results support a strong wording effect but still support the notion of two correlated but distinguishable subdimensions. Finally, our results highlight the importance of differentiating between subjects when investigating grit.
HIWIND Balloon and Antarctica Jang Bogo FPI High Latitude Conjugate Thermospheric Wind Observations and Simulations
Authors: Wu Qian , Lin Dong , Wang Wenbing , Qian Liying , Jee Geonhwa , Lee Changsup , Kim Jeong‐han
Published: May 2024

Using the High attitude Interferometer WIND observation balloon and Antarctic Jang Bogo station high latitude conjugate observations of the thermospheric winds we investigate the seasonal and hemispheric differences between the northern and southern hemispheres in June 2018. We found that the summer (northern) hemisphere dayside meridional winds have a double‐hump feature, whereas in the winter (southern) hemisphere the dayside meridional winds have a single hump feature. We attribute that to stronger summer, perhaps, northern hemisphere cusp heating. We also compared the observation with NCAR Thermosphere Ionosphere Electrodynamics General Circulation Model (TIEGCM) model. The TIEGCM reproduced the double‐hump feature because of added cusp heating. The summer hemisphere has stronger anti‐sunward winds. This is the first time we have very high latitude conjugate thermospheric wind observations.
Unexpectedly High Magma Productivity Inferred From Crustal Roughness and Residual Bathymetry on the Eastern Part of the Ultra‐Slow Spreading Gakkel Ridge Since ∼45 Ma, Eurasian Basin, Arctic Ocean
Authors: Tan Pingchuan , Breivik Asbjørn Johan , Ding Weiwei , Zhang Tao , Niu Xiongwei , Li Jiabiao
Published: May 2024

The Gakkel Ridge in the Eurasian Basin has the slowest seafloor spreading worldwide. The western Gakkel Ridge (3°W–85°E; 14–11 mm/a) alternate between magmatic and sparsely magmatic zones, while the eastern Gakkel Ridge (85–126°E; 11–6 mm/a) appears to be dominated by magmatic zones despite ultraslow spreading. Little is known about the seafloor spreading conditions in the past along the entire ridge. Here, we exploit the residual bathymetry and basement roughness to assess the crustal accretion process of the Gakkel Ridge over time using 23 published regional multichannel seismic reflection profiles. Full seafloor spreading rates were faster (20–24 mm/a) up to ∼45 Ma, and residual bathymetry for the older crust is deeper than the world average in the entire Eurasian Basin. There is a sharp transition to 300–400 m shallower residual bathymetry for seafloor <45 Ma in the eastern Eurasian Basin. The crustal roughness versus spreading rate of the western Eurasian Basin is on the global trend, while that of the eastern is significantly below. Both low roughness and shallow residual bathymetry of the eastern Eurasian Basin is close to that of oceanic crust for spreading rates above 30 mm/a, demonstrating increased magmatic production of the eastern Gakkel Ridge since ∼45 Ma. A recent mantle tomography model predicts partial melting in the upper mantle based on the low Vs anomaly underneath. The sedimentary pattern toward the Lomonosov Ridge indicates that this hot mantle anomaly started to cause dynamic uplift of the area at ∼45 Ma.
Variability of Iceland Scotland Overflow Water Across the Reykjanes Ridge: 2‐Years of Moored Observations in the Bight Fracture Zone
Authors: Furey H. , Bower A. , Ramsey A. , Houk A. , Meunier T.
Published: May 2024

This study presents the first continuous observations of Iceland Scotland Overflow Water (ISOW) passing through the Bight Fracture Zone (BFZ), the northernmost deep bathymetric channel across the Reykjanes Ridge between the Iceland and Irminger Basins in the subpolar North Atlantic. Data from two 2‐year moorings, measuring temperature, salinity, and current velocity from 2015 to 2017, along with a set of deep ISOW‐embedded RAFOS floats, are used to investigate ISOW transport and water property variability through the BFZ, as well as advective pathways between the Iceland and Irminger Basins. The mooring‐derived record‐mean ISOW transport through the BFZ was −0.59 ± 0.27 × 1e6 m3/s (westward) and varied seasonally with weaker transport in winter and stronger transport in summer. Flow direction of ISOW through the BFZ was consistently westward except in winter, when week‐long flow reversals were frequently observed. The previously reported subpolar North Atlantic freshening event of the 2010s is evident in the BFZ mooring records beginning about January 2017. About one‐quarter of floats deployed in ISOW at 1800‐m depth upstream in the Iceland Basin show a direct advective pathway into the BFZ that appears to be primarily determined by bathymetry. Another quarter of the floats crossed over the ridge to the Irminger Sea through other gaps prior to reaching the Charlie‐Gibbs Fracture Zone.
Bare Patches Created by Plateau Pikas Contribute to Warming Permafrost on the Tibet Plateau
Authors: Chen Yuhong , Nan Zhuotong , Zhao Shuping
Published: May 2024

Plateau pikas, small mammals native to the Qinghai‐Tibet Plateau (QTP), create bare patches through burrowing. No previous assessment exists on their impact on permafrost. This study fills this gap by simulating hypothetical scenarios in the Three Rivers Headwaters Region of the QTP using the Noah‐MP model for the plant growing seasons during 2015–2018. Our findings reveal a significant increase in soil temperature in the active layer due to pika‐induced bare patches, particularly during July–August. The average temperature rise at 2.5 cm depth was 0.36°C in permafrost regions and 0.29°C in seasonally frozen ground regions during August. Minimal impact on unfrozen water content was observed, with a slight increase in deep soil layers in permafrost regions, and negligible in seasonally frozen areas. These findings underscore the previously unexplored influence of pika burrowing on permafrost temperature, suggesting a potential risk of accelerating permafrost degradation, especially in permafrost‐dominated regions.
Longer‐ and Slower‐Moving Contiguous Heatwaves Linked to El Niño
Authors: Wu Sijia , Luo Ming , Liu Zhen , Wang Xiaoyu , Huang Ziwei , Li Xiang
Published: May 2024

Although it is known that the frequency and intensity of heatwaves are affected by the El Niño–Southern Oscillation (ENSO), unknown are the ENSO modulations on the moving properties (e.g., moving distance and speed) of spatiotemporally contiguous heatwaves. Here, we investigate the relationship between ENSO and the moving patterns of contiguous heatwaves. We show that contiguous heatwaves are likely more frequent, more persistent, and longer‐traveling, but slower‐moving during El Niño than La Niña episodes. The differences in the tropical contiguous heatwaves between El Niño and La Niña are influenced by persistent high‐pressure anomalies. During the following summers, El Niño can induce anomalous atmospheric circulation characterized by an intensified subsidence over the western North Pacific and ascending motion over the tropical Indian and Pacific Oceans. These features provide favorable conditions for the occurrence and maintenance of contiguous heatwaves.
Disentangling heterogeneity in substance use disorder: Insights from genome-wide polygenic scores
Authors: Vilar-Ribó Laura , Cabana-Domínguez Judit , Alemany Silvia , Llonga Natalia , Arribas Lorena , Grau-López Lara , Daigre Constanza , Cormand Bru , Fernàndez-Castillo Noèlia , Ramos-Quiroga Josep Antoni , Soler Artigas María , Ribasés Marta
Published: May 2024

Substance use disorder (SUD) is a global health problem with a significant impact on individuals and society. The presentation of SUD is diverse, involving various substances, ages at onset, comorbid conditions, and disease trajectories. Current treatments for SUD struggle to address this heterogeneity, resulting in high relapse rates. SUD often co-occurs with other psychiatric and mental health-related conditions that contribute to the heterogeneity of the disorder and predispose to adverse disease trajectories. Family and genetic studies highlight the role of genetic and environmental factors in the course of SUD, and point to a shared genetic liability between SUDs and comorbid psychopathology. In this study, we aimed to disentangle SUD heterogeneity using a deeply phenotyped SUD cohort and polygenic scores (PGSs) for psychiatric disorders and related traits. We explored associations between PGSs and various SUD-related phenotypes, as well as PGS-environment interactions using information on lifetime emotional, physical, and/or sexual abuse. Our results identify clusters of individuals who exhibit differences in their phenotypic profile and reveal different patterns of associations between SUD-related phenotypes and the genetic liability for mental health-related traits, which may help explain part of the heterogeneity observed in SUD. In our SUD sample, we found associations linking the genetic liability for attention-deficit hyperactivity disorder (ADHD) with lower educational attainment, the genetic liability for post-traumatic stress disorder (PTSD) with higher rates of unemployment, the genetic liability for educational attainment with lower rates of criminal records and unemployment, and the genetic liability for well-being with lower rates of outpatient treatments and fewer problems related to family and social relationships. We also found evidence of PGS-environment interactions showing that genetic liability for suicide attempts worsened the psychiatric status in SUD individuals with a history of emotional physical and/or sexual abuse. Collectively, these data contribute to a better understanding of the role of genetic liability for mental health-related conditions and adverse life experiences in SUD heterogeneity.
The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma
Authors: Cheng Aoming , Xu Qiaoshi , Li Bo , Zhang Lirui , Wang Hao , Liu Chang , Han Zhengxue , Feng Zhien
Published: May 2024

The tumor margin as the invasive front has been proven to be closely related to the progression and metastasis of oral squamous cell carcinoma (OSCC). However, how tumor cells in the marginal region obtain the extra energy needed for tumor progression is still unknown. Here, we used spatial metabolomics and the spatial transcriptome to identify enhanced energy metabolism in the tumor margin of OSCC and identified that the downregulation of Ras-related glycolysis inhibitor and calcium channel regulator (RRAD) in tumor cells mediated this process. The absence of RRAD enhanced the ingestion of glucose and malignant behaviors of tumor cells both in vivo and in vitro. Mechanically, the downregulation of RRAD promoted the internal flow of Ca2+ and elevated its concentration in the nucleus, which resulted in the activation of the CAMKIV-CREB1 axis to induce the transcription of the glucose transporter GLUT3. GLUT inhibitor-1, as an inhibitor of GLUT3, could suppress this vigorous energy metabolism and malignant behaviors caused by the downregulation of RRAD. Taken together, our study revealed that enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of OSCC and proved that GLUT3 is a potential target for future treatment of OSCC.
HAX-1 interferes in assembly of NLRP3-ASC to block microglial pyroptosis in cerebral I/R injury
Authors: Guo Xin-bin , Deng Xin , Wang Jingjing , Qi Yuruo , Zhao Wen , Guan Sheng
Published: May 2024

Acute cerebral ischemia has a high rate of disability and death. Although timely recanalization therapy may rescue the ischemic brain tissue, cerebral ischemia-reperfusion injury has been shown to limit the therapeutic effects of vascular recanalization. Protein HAX-1 has been reported as a pro-survival protein that plays an important role in various disorders, particularly in association with the nervous system. However, the effects and mechanisms of HAX-1 in cerebral IR injury have yet to be elucidated. So, we aimed to investigate the effect of HAX-1 on microglial pyroptosis and explore its potential neuroprotective effects in ischemia-reperfusion injury. Our results show that the expression of HAX-1 decreased after cerebral IR injury, accompanied by an increase in pyroptosis pathway activation. In addition, HAX-1 could inhibit microglial pyroptosis both in vivo and in vitro and reduce the release of inflammatory mediators. The above neuroprotective effects might be partially mediated by inhibiting of interaction of NLRP3 and ASC through competitive binding, followed by the attenuation of NLRP3 inflammasome formation. In conclusion, Our findings support that HAX-1 exhibits a protective role in cerebral I/R injury, and further study on HAX-1 expression regulation will contribute to cerebral infarction therapy.
Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing
Authors: Qin Peiliang , Zhou Peng , Huang Yating , Long Binbin , Gao Ruikang , Zhang Shan , Zhu Bingjie , Li Yi-Qing , Li Qin
Published: May 2024

Diabetic foot ulcer (DFU) is a prevalent complication of diabetes that poses significant challenges in terms of treatment and management. It is characterized by heightened endothelial apoptosis and impaired angiogenesis. In this study, we aimed to investigate the role of protein kinase Cδ (PKCδ) in regulating endothelial apoptosis in diabetic wounds by promoting cholesterol biosynthesis. The expression of PKCδ was increased in human umbilical vascular endothelial cells (HUVECs) cultivated in high glucose medium and skin tissue isolated from diabetic mice. High glucose-induced HUVECs apoptosis was reduced by PKCδ inhibition with siRNA or rottlerin. RNA-seq identified two enzymes, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), as the downstream of PKCδ. PKCδ knockdown or inhibition suppressed the expression of HMGCS1 and HMGCR and lowered free cholesterol (FC) levels. Cholesterol restored high glucose-induced apoptosis in siRNA- or rottlerin-treated HUVECs. In vivo use of rosuvastatin calcium, an inhibitor of HMGCR, downregulated free cholesterol levels and accelerated the wound healing process. In conclusion, PKCδ expression in endothelial cells was activated by high glucose, which subsequently upregulates the expression of two enzymes catalyzing cholesterol biosynthesis, HMGCS1 and HMGCR. Enhanced cholesterol biosynthesis raises free cholesterol levels, promotes endothelial apoptosis, and finally delays wound healing.
Hypoxia-induced epigenetic regulation of miR-485-3p promotes stemness and chemoresistance in pancreatic ductal adenocarcinoma via SLC7A11-mediated ferroptosis
Authors: Liu Xinxin , Huang Zhihua , Chen Qiuzheng , Chen Kai , Liu Weikang , Liu Guangnian , Chu Xiangyu , Li Dongqi , Ma Yongsu , Tian Xiaodong , Yang Yinmo
Published: May 2024

The mechanism of hypoxia in chemoresistance of pancreatic ductal adenocarcinoma (PDAC) remains elusive. In this study, we revealed the essential role of miR-485-3p in PDAC, particularly its impact on cancer stemness and gemcitabine resistance under hypoxic conditions. We found substantial downregulation of miR-485-3p in PDAC tissues, with lower expression correlating to poor patient outcomes. Mechanistically, miR-485-3p influenced stemness characteristics, as evidenced by reduced tumor-sphere formation and increased sensitivity to gemcitabine upon overexpression. Moreover, we identified SOX9 and SLC7A11 as two targets of miR-485-3p, which play a vital role in stemness and ferroptosis. Under the hypoxic condition, DNMT3B expression was upregulated, leading to hypermethylation of the miR-485-3p promoter region. The reduced miR-485-3p expression promoted stemness and chemoresistance of PDAC. In conclusion, our findings elucidate the intricate interplay of hypoxia, epigenetic modifications, and ferroptosis in PDAC and shed light on potential avenues for targeted interventions that modulate cancer stemness and chemosensitivity, offering prospects for improved therapeutic strategies for PDAC.
Remote sensing of emperor penguin abundance and breeding success
Authors: Winterl Alexander , Richter Sebastian , Houstin Aymeric , Barracho Téo , Boureau Matthieu , Cornec Clément , Couet Douglas , Cristofari Robin , Eiselt Claire , Fabry Ben , Krellenstein Adélie , Mark Christoph , Mainka Astrid , Ménard Delphine , Morinay Jennifer , Pottier Susie , Schloesing Elodie , Le Bohec Céline , Zitterbart Daniel P.
Published: May 2024

Emperor penguins (Aptenodytes forsteri) are under increasing environmental pressure. Monitoring colony size and population trends of this Antarctic seabird relies primarily on satellite imagery recorded near the end of the breeding season, when light conditions levels are sufficient to capture images, but colony occupancy is highly variable. To correct population estimates for this variability, we develop a phenological model that can predict the number of breeding pairs and fledging chicks, as well as key phenological events such as arrival, hatching and foraging times, from as few as six data points from a single season. The ability to extrapolate occupancy from sparse data makes the model particularly useful for monitoring remotely sensed animal colonies where ground-based population estimates are rare or unavailable.
Photo-cycloaddition reactions of vinyldiazo compounds
Authors: Bao Ming , Łuczak Klaudia , Chaładaj Wojciech , Baird Marriah , Gryko Dorota , Doyle Michael P.
Published: May 2024

Heterocyclic rings are important structural scaffolds encountered in both natural and synthetic compounds, and their biological activity often depends on these motifs. They are predominantly accessible via cycloaddition reactions, realized by either thermal, photochemical, or catalytic means. Various starting materials are utilized for this purpose, and, among them, diazo compounds are often encountered, especially vinyldiazo compounds that give access to donor-acceptor cyclopropenes which engage in [2+n] cycloaddition reactions. Herein, we describe the development of photochemical processes that produce diverse heterocyclic scaffolds from multisubstituted oximidovinyldiazo compounds. High chemoselectivity, good functional group tolerance, and excellent scalability characterize this methodology, thus predisposing it for broader applications. Experimental and computational studies reveal that under light irradiation these diazo reagents selectively transform into cyclopropenes which engage in cycloaddition reactions with various dipoles, while under thermal conditions the formation of pyrazole from vinyldiazo compounds is favored.
PIF transcriptional regulators are required for rhythmic stomatal movements
Authors: Rovira Arnau , Veciana Nil , Basté-Miquel Aina , Quevedo Martí , Locascio Antonella , Yenush Lynne , Toledo-Ortiz Gabriela , Leivar Pablo , Monte Elena
Published: May 2024

Stomata govern the gaseous exchange between the leaf and the external atmosphere, and their function is essential for photosynthesis and the global carbon and oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night and allow CO2 uptake during the day. How the actors involved are transcriptionally regulated and how this might contribute to rhythmicity is largely unknown. Here, we show that morning stomata opening depends on the previous night period. The transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) accumulate at the end of the night and directly induce the guard cell-specific K+ channel KAT1. Remarkably, PIFs and KAT1 are required for blue light-induced stomata opening. Together, our data establish a molecular framework for daily rhythmic stomatal movements under well-watered conditions, whereby PIFs are required for accumulation of KAT1 at night, which upon activation by blue light in the morning leads to the K+ intake driving stomata opening.
Organic and inorganic sublattice coupling in two-dimensional lead halide perovskites
Authors: Fu Jianhui , Bian Tieyuan , Yin Jun , Feng Minjun , Xu Qiang , Wang Yue , Sum Tze Chien
Published: May 2024

Two-dimensional layered organic-inorganic halide perovskites have successfully spread to diverse optoelectronic applications. Nevertheless, there remain gaps in our understanding of the interactions between organic and inorganic sublattices that form the foundation of their remarkable properties. Here, we examine these interactions using pump-probe spectroscopy and ab initio molecular dynamics simulations. Unlike off-resonant pumping, resonant excitation of the organic sublattice alters both the electronic and lattice degrees of freedom within the inorganic sublattice, indicating the existence of electronic coupling. Theoretical simulations verify that the reduced bandgap is likely due to the enhanced distortion index of the inorganic octahedra. Further evidence of the mechanical coupling between these two sublattices is revealed through the slow heat transfer process, where the resultant lattice tensile strain launches coherent longitudinal acoustic phonons. Our findings explicate the intimate electronic and mechanical couplings between the organic and inorganic sublattices, crucial for tailoring the optoelectronic properties of two-dimensional halide perovskites.
Glucocorticoids paradoxically promote steroid resistance in B cell acute lymphoblastic leukemia through CXCR4/PLC signaling
Authors: Abdoul-Azize Souleymane , Hami Rihab , Riou Gaetan , Derambure Céline , Charbonnier Camille , Vannier Jean-Pierre , Guzman Monica L. , Schneider Pascale , Boyer Olivier
Published: May 2024

Glucocorticoid (GC) resistance in childhood relapsed B-cell acute lymphoblastic leukemia (B-ALL) represents an important challenge. Despite decades of clinical use, the mechanisms underlying resistance remain poorly understood. Here, we report that in B-ALL, GC paradoxically induce their own resistance by activating a phospholipase C (PLC)-mediated cell survival pathway through the chemokine receptor, CXCR4. We identify PLC as aberrantly activated in GC-resistant B-ALL and its inhibition is able to induce cell death by compromising several transcriptional programs. Mechanistically, dexamethasone (Dex) provokes CXCR4 signaling, resulting in the activation of PLC-dependent Ca2+ and protein kinase C signaling pathways, which curtail anticancer activity. Treatment with a CXCR4 antagonist or a PLC inhibitor improves survival of Dex-treated NSG mice in vivo. CXCR4/PLC axis inhibition significantly reverses Dex resistance in B-ALL cell lines (in vitro and in vivo) and cells from Dex resistant ALL patients. Our study identifies how activation of the PLC signalosome in B-ALL by Dex limits the upfront efficacy of this chemotherapeutic agent.
Germline Cas9 promoters with improved performance for homing gene drive
Authors: Du Jie , Chen Weizhe , Jia Xihua , Xu Xuejiao , Yang Emily , Zhou Ruizhi , Zhang Yuqi , Metzloff Matt , Messer Philipp W. , Champer Jackson
Published: May 2024

Gene drive systems could be a viable strategy to prevent pathogen transmission or suppress vector populations by propagating drive alleles with super-Mendelian inheritance. CRISPR-based homing gene drives convert wild type alleles into drive alleles in heterozygotes with Cas9 and gRNA. It is thus desirable to identify Cas9 promoters that yield high drive conversion rates, minimize the formation rate of resistance alleles in both the germline and the early embryo, and limit somatic Cas9 expression. In Drosophila, the nanos promoter avoids leaky somatic expression, but at the cost of high embryo resistance from maternally deposited Cas9. To improve drive efficiency, we test eleven Drosophila melanogaster germline promoters. Some achieve higher drive conversion efficiency with minimal embryo resistance, but none completely avoid somatic expression. However, such somatic expression often does not carry detectable fitness costs for a rescue homing drive targeting a haplolethal gene, suggesting somatic drive conversion. Supporting a 4-gRNA suppression drive, one promoter leads to a low drive equilibrium frequency due to fitness costs from somatic expression, but the other outperforms nanos, resulting in successful suppression of the cage population. Overall, these Cas9 promoters hold advantages for homing drives in Drosophila species and may possess valuable homologs in other organisms.
Reversible flexoelectric domain engineering at the nanoscale in van der Waals ferroelectrics
Authors: Liu Heng , Lai Qinglin , Fu Jun , Zhang Shijie , Fu Zhaoming , Zeng Hualing
Published: May 2024

The universal flexoelectric effect in solids provides a mechanical pathway for controlling electric polarization in ultrathin ferroelectrics, eliminating potential material breakdown from a giant electric field at the nanoscale. One challenge of this approach is arbitrary implementation, which is strongly hindered by one-way switching capability. Here, utilizing the innate flexibility of van der Waals materials, we demonstrate that ferroelectric polarization and domain structures can be mechanically, reversibly, and arbitrarily switched in two-dimensional CuInP2S6 via the nano-tip imprinting technique. The bidirectional flexoelectric control is attributed to the extended tip-induced deformation in two-dimensional systems with innate flexibility at the atomic scale. By employing an elastic substrate, artificial ferroelectric nanodomains with lateral sizes as small as ~80 nm are noninvasively generated in an area of 1 μm2, equal to a density of 31.4 Gbit/in2. Our results highlight the potential applications of van der Waals ferroelectrics in data storage and flexoelectronics.
The applications of flexible electronics in dental, oral, and craniofacial medicine
Authors: Wang Kang-Ning , Li Zi-Zhan , Cai Ze-Min , Cao Lei-Ming , Zhong Nian-Nian , Liu Bing , Zhou Kan , Huo Fang-Yi , Cai Bo , Bu Lin-Lin
Published: May 2024

Dental, oral, and craniofacial diseases jeopardize health and reduce the quality of life. Accessing disease-related signals in advance is beneficial to prevent the occurrence or progression of those diseases. However, the inconvenience of periodical in-hospital examinations and the difficulty of sustaining daily health monitoring challenge personal compliance and possibly lead to limited prevention or treatment. Medical flexible electronics are electric devices fabricated on soft and extensible substrates to fit the human skin and enable non-invasive continuous monitoring of biophysical/biochemical signals. They provide the possibility of long-term, continuous, comfortable, and wireless healthcare monitoring and are expected to alleviate time and economic consumption by avoiding in-hospital examinations and treatment. Therefore, flexible electronics have emerged for early diagnosis and disease monitoring in stomatology. It is noteworthy that special biophysical/biochemical characteristics and the environment of dental, oral, and craniofacial areas bring distinct challenges that flexible electronics need to address ingeniously to ensure their stability, selectivity, and sensitivity. This review summaries flexible electronics and their specificity when used in dental, oral, and craniofacial applications, including monitoring saliva or cavity-gas related biosignals, sensing the mechanical fluctuation from facial muscle/respiratory activities or orthodontic forces, and executing special functions in the prevention or postoperative recovery of relevant diseases. Furthermore, after analyzing current challenges and proposing potential solutions, the “5I” principles of imperceptibility, intelligence, individualization, integration, and inexpensiveness are presented to help guide the future development of flexible electronics and promote their commercialization for dental, oral, and craniofacial medicine.
Inferring upstream regulatory genes of FOXP3 in human regulatory T cells from time-series transcriptomic data
Authors: Magni Stefano , Sawlekar Rucha , Capelle Christophe M. , Tslaf Vera , Baron Alexandre , Zeng Ni , Mombaerts Laurent , Yue Zuogong , Yuan Ye , Hefeng Feng Q. , Gonçalves Jorge
Published: May 2024

The discovery of upstream regulatory genes of a gene of interest still remains challenging. Here we applied a scalable computational method to unbiasedly predict candidate regulatory genes of critical transcription factors by searching the whole genome. We illustrated our approach with a case study on the master regulator FOXP3 of human primary regulatory T cells (Tregs). While target genes of FOXP3 have been identified, its upstream regulatory machinery still remains elusive. Our methodology selected five top-ranked candidates that were tested via proof-of-concept experiments. Following knockdown, three out of five candidates showed significant effects on the mRNA expression of FOXP3 across multiple donors. This provides insights into the regulatory mechanisms modulating FOXP3 transcriptional expression in Tregs. Overall, at the genome level this represents a high level of accuracy in predicting upstream regulatory genes of key genes of interest.
A narrative review of norovirus epidemiology, biology, and challenges to vaccine development
Authors: Carlson Katherine B. , Dilley Anne , O’Grady Thomas , Johnson Jordan A. , Lopman Ben , Viscidi Emma
Published: May 2024

Norovirus is a leading cause of acute gastroenteritis (AGE) globally. AGE resulting from norovirus causes significant morbidity and mortality in countries of all income levels, particularly among young children and older adults. Prevention of norovirus AGE represents a unique challenge as the virus is genetically diverse with multiple genogroups and genotypes cocirculating globally and causing disease in humans. Variants of the GII.4 genotype are typically the most common genotype, and other genotypes cause varying amounts of disease year-to-year, with GII.2, GII.3, and GII.6 most prevalent in recent years. Noroviruses are primarily transmitted via the fecal-oral route and only a very small number of virions are required for infection, which makes outbreaks of norovirus extremely difficult to control when they occur. Settings like long-term care facilities, daycares, and hospitals are at high risk of outbreaks and can have very high attack rates resulting in substantial costs and disease burden. Severe cases of norovirus AGE are most common in vulnerable patient populations, such as infants, the elderly, and immunocompromised individuals, with available treatments limited to rehydration therapies and supportive care. To date, there are no FDA-approved norovirus vaccines; however, several candidates are currently in development. Given the substantial human and economic burden associated with norovirus AGE, a vaccine to prevent morbidity and mortality and protect vulnerable populations could have a significant impact on global public health.
Dataset of Finite Element Models of Normal and Deformed Thoracolumbar Spine
Authors: Rasouligandomani Morteza , del Arco Alejandro , Chemorion Francis Kiptengwer , Bisotti Marc-Antonio , Galbusera Fabio , Noailly Jérôme , González Ballester Miguel A.
Published: May 2024

Adult spine deformity (ASD) is prevalent and leads to a sagittal misalignment in the vertebral column. Computational methods, including Finite Element (FE) Models, have emerged as valuable tools for investigating the causes and treatment of ASD through biomechanical simulations. However, the process of generating personalised FE models is often complex and time-consuming. To address this challenge, we present a dataset of FE models with diverse spine morphologies that statistically represent real geometries from a cohort of patients. These models are generated using EOS images, which are utilized to reconstruct 3D surface spine models. Subsequently, a Statistical Shape Model (SSM) is constructed, enabling the adaptation of a FE hexahedral mesh template for both the bone and soft tissues of the spine through mesh morphing. The SSM deformation fields facilitate the personalization of the mean hexahedral FE model based on sagittal balance measurements. Ultimately, this new hexahedral SSM tool offers a means to generate a virtual cohort of 16807 thoracolumbar FE spine models, which are openly shared in a public repository.
Detection of differential bait proteoforms through immunoprecipitation-mass spectrometry data analysis
Authors: Kourtis Savvas , Cianferoni Damiano , Serrano Luis , Sdelci Sara
Published: May 2024

Proteins are often referred to as the workhorses of cells, and their interactions are necessary to facilitate specific cellular functions. Despite the recognition that protein-protein interactions, and thus protein functions, are determined by proteoform states, such as mutations and post-translational modifications (PTMs), methods for determining the differential abundance of proteoforms across conditions are very limited. Classically, immunoprecipitation coupled with mass spectrometry (IP-MS) has been used to understand how the interactome (preys) of a given protein (bait) changes between conditions to elicit specific cellular functions. Reversing this concept, we present here a new workflow for IP-MS data analysis that focuses on identifying the differential peptidoforms of the bait protein between conditions. This method can provide detailed information about specific bait proteoforms, potentially revealing pathogenic protein states that can be exploited for the development of targeted therapies.
Integrated coupled assessment of geostorage and geothermal prospects in the oil fields of Upper Assam Basin
Authors: Dutta Anupal Jyoti , Gogoi Nababrot , Hussain Firdush Zallah , Kulkarni Sandeep D.
Published: May 2024

This study proposes an integrated approach of assessing CO2storage potential and geothermal energy prospect based on the data of seventeen depleted wells of Upper Assam Basin which could assist the global objective of net zero transition. The petrophysical properties of Tipam, Barail and Lakadong + Therria Formations from the seventeen wells have been utilised to perform the Monte Carlo simulation for probabilistic estimation of the CO2storage in the Upper Assam Basin. This preliminary work showed that the mean storage capacity of 18.8 ± 0.7 MT, 19.8 ± 0.9 MT and 4.5 ± 0.8 MT could potentially be stored in the three geological formations of the basin. The corrected bottom hole temperature values for the studied seventeen wells were determined using the well log data and Waples and Harrison method; these values provided a static geothermal gradient for each well, which varies widely from 0.017 to 0.033 °C/m. In order to enable geothermal prospectivity, static formation temperature maps have been generated for the studied wells. The probabilistic assessment of stored heat-in-place and formation temperature maps delimited five prospective sites for the extraction of geothermal energy in the basin. The study also presented a risk assessment for CO2storage development in the basin. Further, the study illustrated an economic analysis of the implementation of a CO2storage project and geothermal operations in the basin.