bluuebunny/crossref_metadata_2025 huggingface dataset:
Learning from multi-paradigmatic sensitivity in cross-cultural management? Empirical and theoretical considerations
Authors: Grosskopf Sina , Barmeyer Christoph
Published: June 2021

Paradigms are basic assumptions about how social reality is perceived, understood and explained. Whereas most research is based on a single paradigm, few empirical papers show the advantages of using multiple paradigms within a study. This article pleads for multi-paradigm studies in cross-cultural management research in order to reach a more multifaceted representation of cultural phenomena. This is particularly consistent with the field of cross-cultural management, because it would be ethnocentric to consider intercultural situations only from one perspective, usually that of one’s own culture. The argument corresponds to the ambition of cross-cultural management to respect and adopt multiple (cultural) perspectives and, analogously, to achieve a ‘paradigmatic ethnorelativism’. Based on an intercultural situation, and therefore going beyond meta-theoretical reasoning, this article demonstrates multi-paradigmatic sensitivity in terms of the functionalist, interpretive and critical paradigms. The use of these theoretical concepts leads to multiple angles and a less ‘ethnocentric’ position, and hence to more nuanced knowledge creation with regard to the intercultural situation. The ‘blind spots’ of each paradigm, but also their complementarities, are discussed. Consequently, this article raises theoretical and practical implications for cross-cultural management by offering a way to a richer understanding of intercultural situations through openness to different paradigms.
Does the CEO effect on performance differ in private versus public firms?
Authors: Quigley Timothy J. , Chirico Francesco , Baù Massimo
Published: June 2021

Scholars have long debated the effect CEOs have on firm performance, including a focus on how their effect shifts across industries, national settings, and time. Unexplored, however, is the possibility that the CEO effect might differ in publicly traded versus privately held firms. Drawing on a unique longitudinal sample of both publicly traded and large, privately held Swedish firms from 1997 to 2013, we replicate and build upon prior CEO effects studies and find that private-firm CEOs have a greater effect on firm performance, for good or for ill, than do their public firm counterparts. Our results are strengthened after controlling for industry, firm profitability, and size in a matched-pair sample. We discuss the implications and potential future research stemming from these findings.
RNA Binding Protein-Based Model for Prognostic Prediction of Colorectal Cancer
Authors: Li Ting , Hui Wenjia , Halike Halina , Gao Feng
Published: June 2021

Background:

Colorectal cancer (CRC) is a kind of gastrointestinal tumor with serious high morbidity and mortality. Several reports have implicated the disorder of RNA-binding proteins (RBPs) in plenty of tumors, associating it to tumorigenesis and disease progression. The study is intended to construct novel prognostic biomarkers associated with CRC patients.

Methods:

Data of gene expression was acquired from the TCGA database, prognosis-related genes were selected. Besides, we analyzed GO and KEGG pathways. Univariate and multivariate Cox analyses were performed to generate a prognostic-related gene signature, which was evaluated by the Kaplan-Meier (K-M) and the Receiver Operating Characteristic (ROC) curve. The independent prognostic factor was established by survival analysis. GSE38832 dataset was used to validate the signature. Finally, expression of 8 genes was further confirmed by qRT-PCR in SW480 and SW620 cell lines.

Results:

We obtained 224 differentially expressed RBPS in total, of which 78 were downregulated and 146 were upregulated. Univariate COX analysis was conducted in the TCGA cohort to select 13 RBPs with P < 0.005, stepwise multivariate COX regression analysis was used to construct an 8—RBP signature (TERT, PPARGC1A, BRCA1, CELF4, TDRD7, LUZP4, PNLDC1, ZC3H12C). Based on the model, systematic analysis illustrated that a high risk score was obviously connected to a poor prognosis. The prognostic value of the risk score was validated in GSE38832 dataset, indicating that the risk model was accurate and effective. The prognostic signature-based risk score was identified as an independent prognostic indicator for CRC. The expression results of qRT-PCR were consistent with the results of differential expression analysis.

Conclusions:

The eight-RBP signature can predict the survival of CRC patients and potentially act as CRC prognostic biomarker.
Analysis of Serum miRNAs in Alzheimer’s Disease
Authors: Lu Liu , Dai Wen-Zhuo , Zhu Xi-Chen , Ma Tao
Published: June 2021

This paper was aimed to analyze the microRNA (miRNA) signatures in Alzheimer disease (AD) and find the significant expressions of miRNAs, their target genes, the functional enrichment analysis of the confirmed genes, and potential drug treatment. The miRNA expression information of the gene expression profile data was downloaded from the Gene Expression Omnibus database. The total data sample size is 1309, including 1021 AD samples and 288 normal samples. A total of 21 differentially expressed miRNAs were obtained, of which 16 (hsa-miR-6761-3p, hsa-miR-6747-3p, hsa-miR-6875-3p, hsa-miR-6754-3p, hsa-miR-6736-3p, hsa-miR-6762-3p, hsa-miR-6787-3p, hsa-miR-208a-5p, hsa-miR-6740-3p, hsa-miR-6778-3p, hsa-miR-595, hsa-miR-6753-3p, hsa-miR-4747-3p, hsa-miR-3646, hsa-miR-6716-3p and hsa-miR-4435) were up-regulated and 5 (hsa-miR-125a-3p, hsa-miR-22-3p, hsa-miR-24-3p, hsa-miR-6131 and hsa-miR-125b-1-3p) were down-regulated in AD. A total of 6 miRNAs (hsa-miR-595, hsa-miR-3646, hsa-miR-4435 hsa-miR-125a-3p, hsa-miR-22-3p and hsa-miR-24-3p) and 78 miRNA-disease-related gene sub-networks were predicted, and 116 ceRNA regulatory relationship pairs, and the ceRNA regulatory network were obtained. The results of enrichment analysis suggested that the main target pathways of several miRNAs differentially expressed in AD were mitogen-activated protein kinase signal pathway. According to the prediction results of Drug-Gene Interaction database 2.0, we obtained 53 pairs of drug-gene interaction, including 7 genes (PTGS2, EGFR, CALM1, PDE4D, FGFR2, HMGCR, cdk6) and 53 drugs. We hope our results are helpful to find a viable way to prevent, delay the onset, diagnose, and treat AD.
Differential expression and alternative splicing of transcripts in orbital adipose/connective tissue of thyroid-associated ophthalmopathy
Authors: Wu Lianqun , Liang Yu , Song Nan , Wang Xiying , Jiang Chao , Chen Xinxin , Qin Bing , Sun Xiantao , Liu Guohua , Zhao Chen
Published: June 2021

Thyroid-associated ophthalmopathy is a typical autoimmune disease of orbital tissues. Alternative splicing significantly influences many diseases progression, including cancer, age-related macular degeneration, and multiple sclerosis, by modulating the expression of transcripts. However, its role in thyroid-associated ophthalmopathy is still unclear. In this study, differential expression transcripts and differential alternative splicing genes in orbital adipose/connective tissues of thyroid-associated ophthalmopathy patients were detected using RNA sequencing, Cuffdiff, and replicate multivariate analysis of transcript splicing. Three thousand ninety six differential expression transcripts and 2355 differential alternative splicing genes were screened out, while functional enrichment analysis indicated that differential expression transcript and differential alternative splicing genes were associated with immune modulation, extracellular matrix remodeling, and adipogenesis. The expression of the SORBS1, SEPT2, COL12A1, and VCAN gene transcripts was verified by qRT-PCR. In conclusion, prevalent alternative splicing is involved in the disease development in thyroid-associated ophthalmopathy. More attention should be paid to the mechanism of alternative splicing to explore more potential therapeutic targets in thyroid-associated ophthalmopathy.
Wall Shear Stress Assessment of the False Lumen in Acute Type B Aortic Dissection Visualized by 4-Dimensional Flow Magnetic Resonance Imaging: An Ex-Vivo Study
Authors: Veger Hugo T. C. , Pasveer Erik H. , Westenberg Jos J. M. , Wever Jan J. , van Eps Randolph G. Statius
Published: June 2021

Background:

Four-dimensional flow magnetic resonance imaging (4D flow MRI) can accurately visualize and quantify flow and provide hemodynamic information such as wall shear stress (WSS). This imaging technique can be used to obtain more insight in the hemodynamic changes during cardiac cycle in the true and false lumen of uncomplicated acute Type B Aortic Dissection (TBAD). Gaining more insight of these forces in the false lumen in uncomplicated TBAD during optimal medical treatment, might result in prediction of adverse outcomes.

Methods:

A porcine aorta dissection model with an artificial dissection was positioned in a validated ex-vivo circulatory system with physiological pulsatile flow. 4D flow MR images with 3 set heartrates (HR; 60 bpm, 80 bpm and 100 bpm) were acquired. False lumen volume per cycle (FLV), mean and peak systolic WSS were determined from 4D flow MRI data. For validation, the experiment was repeated with a second porcine aorta dissection model.

Results:

During both experiments an increase in FLV (initial experiment: ΔFLV = 2.05 ml, p < 0.001, repeated experiment: ΔFLV = 1.08 ml, p = 0.005) and peak WSS (initial experiment: ΔWSS = 1.2 Pa, p = 0.004, repeated experiment: ΔWSS = 1.79 Pa, p = 0.016) was observed when HR increased from 60 to 80 bpm. Raising the HR from 80 to 100 bpm, no significant increase in FLV ( p = 0.073, p = 0.139) was seen during both experiments. The false lumen mean WSS increased significant during initial (2.71 to 3.85 Pa; p = 0.013) and non-significant during repeated experiment (3.22 to 4.00 Pa; p = 0.320).

Conclusion:

4D flow MRI provides insight into hemodynamic dimensions including WSS. Our ex-vivo experiments showed that an increase in HR from 60 to 80 bpm resulted in a significant increase of FLV and WSS of the false lumen. We suggest that strict heart rate control is of major importance to reduce the mean and peak WSS in uncomplicated acute TBAD. Because of the limitations of an ex-vivo study, 4D flow MRI will have to be performed in clinical setting to determine whether this imaging model would be of value to predict the course of uncomplicated TBAD.
Retroperitoneal Laparoscopic-Assisted Retrieval of Wall-Penetrating Inferior Vena Cava Filter After Endovascular Techniques Failed: An Initial Clinical Outcome
Authors: Wang Haidong , Liu Zhenhua , Zhu Xiaofei , Liu Jianlong , Man Libo
Published: June 2021

Background:

Inferior vena cava (IVC) filters are commonly used in China to prevent pulmonary embolisms in patients with deep vein thrombosis. However, IVC filter removal is complicated when the filter has penetrated the IVC wall and endovascular techniques usually fail. The purpose of this study was to evaluate the effectiveness and safety of retroperitoneal laparoscopic-assisted retrieval of wall-penetrating IVC filters after endovascular techniques have failed.

Patients and Methods:

We retrospectively evaluated a series of 8 patients who underwent retroperitoneal laparoscopic-assisted retrieval of a wall-penetrating IVC filter between December 2017 and November 2019. All patients had experienced at least 1 failure with endovascular retrieval before the study. The filters were slanted and the proximal retrieval hooks penetrated the posterior lateral IVC wall in all patients on computed tomography. Demographic information, operation parameters, and complications were recorded and analyzed. All patients were followed up for at least 12 months.

Results:

The procedure was successful in all patients. The median surgery time was 53.6 ± 12.7 min and the average blood loss was 45.0 ± 13.5 ml. No serious complication occurred during the patients’ hospitalization, which was an average of 6.4 days. The median follow-up time was 15.1 months, and no patient had deep vein thrombosis recurrence.

Conclusions:

Retroperitoneal laparoscopic-assisted retrieval is a feasible and effective technique, particularly when proximal retrieval hooks penetrate the posterior lateral wall of the IVC after endovascular techniques have failed. To some extent, the development of this technique at our institution has increased the success rate of filter removal and improved patient satisfaction.
Digilantism, discrimination, and punitive attitudes: A digital vigilantism model
Authors: Galleguillos Sebastián
Published: June 2021

In this article, I propose and apply a digital vigilantism model to a specific incident that occurred in Mexico, where the death of two innocent people was filmed through Facebook Live. Using a mixed methods approach and content analysis, I analyzed digilante Facebook posts ( N = 942) coding gender, digital vigilantism categories, discriminatory comments, and punitive attitudes aimed at the perpetrators and the inciter of the lynching. The categories include investigating, blaming, or rebuking, while the discriminatory comments include classism, racism, homophobia, and body-shaming. I coded the punitive attitudes distinguishing four categories: non-physical punishment (calling for God’s wrath and the guilty conscience of the targets), legal sanction, death, and other punishment. The findings reveal the key role gender played in digilantism: females tend to conduct more investigations and low level attacks (blaming) than males, but males tend to perpetrate more harsh attacks (rebuking) than females. The most popular punitive attitude is calling for the death of targets, revealing tensions between legal sanctions and digilantes’ desired punishment. This study suggests the presence of different expressions of discrimination and reasons to engage in digilantism, encompassing both legal and illegal behavior deployed in a mainstream social media platform such as Facebook.
No Difference in Outcomes Following Osteochondral Allograft with Fresh Precut Cores Compared to Hemi-Condylar Allografts
Authors: Markus Danielle H. , Blaeser Anna M. , Hurley Eoghan T. , Mannino Brian J. , Campbell Kirk A. , Jazrawi Laith M. , Alaia Michael J. , Strauss Eric J. , Alaia Erin F.
Published: June 2021

Objective

The purpose of the current study is to evaluate the clinical and radiographic outcomes at early to midterm follow-up between fresh precut cores versus hemi-condylar osteochondral allograft (OCAs) in the treatment of symptomatic osteochondral lesions.

Design

A retrospective review of patients who underwent an OCA was performed. Patient matching between those with OCA harvested from an allograft condyle/patella or a fresh precut allograft core was performed to generate 2 comparable groups. The cartilage at the graft site was assessed with use of a modified Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) scoring system and patient-reported outcomes were collected.

Results

Overall, 52 total patients who underwent OCA with either fresh precut OCA cores ( n = 26) and hemi-condylar OCA ( n = 26) were pair matched at a mean follow-up of 34.0 months (range 12 months to 99 months). The mean ages were 31.5 ± 10.7 for fresh precut cores and 30.9 ± 9.8 for hemi-condylar ( P = 0.673). Males accounted for 36.4% of the overall cohort, and the mean lesion size for fresh precut OCA core was 19.6 mm2 compared to 21.2 mm2 for whole condyle ( P = 0.178). There was no significant difference in patient-reported outcomes including Visual Analogue Scale, Knee Injury and Osteoarthritis Outcome Score for Joint Replacement, and Tegner ( P > 0.5 for each), or in MOCART score (69.2 vs. 68.3, P = 0.93).

Conclusions

This study found that there was no difference in patient-reported clinical outcomes or MOCART scores following OCA implantation using fresh precut OCA cores or size matched condylar grafts at early to midterm follow-up.
Celastrol alleviates LPS-induced inflammation in BMDMs and acute lung injury in mice via inhibition of p-38 MAPK/MK2 signaling
Authors: Chen Zhengxu , Yang Xinyi , Zhang Lu , Li Man , Sun Lei , Qian Feng
Published: June 2021

Objective:

Celastrol is a compound extracted from a medicinal plant Tripterygium wilfordii which has a broad-spectrum anti-inflammatory effect in traditional medicine. However, the effect of celastrol on acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) is still unknown.

Methods:

We reported that celastrol alleviated LPS-induced acute lung injury by H&E staining, MPO activity and the expression of cytokines in broncho-alveolar lavage fluid. The effect of celastrol on bone marrow-derived macrophages (BMDMs) after LPS treatment was measured by ELISA and Western blotting.

Results:

In vivo, celastrol reduced the LPS-induced lung edema and MPO activity of lung tissue. Furthermore, the production of inflammatory cytokines IL-6, TNF-α, and KC in bronchoalveolar lavage was reduced. In vitro, upon treatment of LPS, celastrol dose-dependently inhibited the expression of iNOS in BMDMs. Meanwhile, the expression of IL-6, TNF-α, and KC in BMDMs were also inhibited by celastrol treatment. Furthermore, we found that celastrol attenuated the phosphorylation of p38 MAPK and MK2, and inhibited the interaction between p38 MAPK and MK2.

Conclusion:

Our data indicate that celastrol has an anti-inflammatory effect on LPS-induced inflammatory response in vivo and in vitro, suggesting celastrol is a promising compound for the treatment of ALI and ARDS.
When positive energy meets satirical feminist backfire: Hashtag activism during the COVID-19 outbreak in China
Authors: Yang Yue
Published: June 2021

Amid the eruption of the COVID-19 outbreak in February 2020, the Chinese Communist Party Youth League promoted VTubers on Weibo to diffuse positive energy. However, the female VTuber Jiangshanjiao was appropriated immediately as political satire by netizens to air public grievances. While political satire is widely considered as political resistance in authoritarian states, little research has addressed its combination with feminist narratives and online activism. This article builds on previous literature on the propagandistic nature of positive energy and the Chinese feminist movement to consider how positive energy lying under Jiangshanjiao was deconstructed and how femininity was invoked to serve for broader political purposes in a repressive online environment. Drawing on the framework of online connective action and political satire as a networked practice, this research sheds light on the hashtag #JiangshanjiaoDoYouGetYourPeriod#. This research explores how the satirical hashtag was collectively produced in an Internet trolling culture and contributed to building a collective identity through personalized narratives. Through the feminist hashtag, netizens expressed their multilayered grievances against misogyny, state propaganda, and censorship. However, this article also offers evidence that the satirical hashtag and ambiguity associated with it limited the influence in catalyzing online and offline changes.
Does time since injury and duration matter in the benefits of physical therapy treatment for concussion?
Authors: Moser Rosemarie S , Schatz Philip , Mayer Bridget , Friedman Sarah , Perkins Melissa , Zebrowski Christina , Islam Siffat , Lemke Hannah , James Matthew , Vidal Paul
Published: June 2021

Objective

To determine if there are differences in post-concussion symptom levels depending on 1) when physical therapy treatment is begun after the concussion and 2) the length of treatment.

Method

Retrospective chart review yielded 202 patients who sustained concussions and were referred for physical therapy. Participants/patients were assigned to independent groups based on time elapsed between concussion and physical therapy (0–14, 15–30, 31–60, 61–120, 121–365 days), and on months spent in treatment (1–4). Pre- and post- treatment scores were documented for the following measures: Sport Concussion Assessment Tool (SCAT), Convergence Insufficiency Symptom Survey (CISS), Dizziness Handicap Inventory (DHI), and Modified Clinical Test of Sensory Interaction on Balance (mCTSIB) using ANOVAs, with a Bonferroni-corrected p-value of p < .005.

Results

All patients demonstrated improvements with treatment, with no significant differences in outcomes for time elapsed since injury (SCAT Symptom Score ( p = .80), SCAT Symptom Severity Score ( p = .97), CISS ( p = .61), DHI ( p = .65), mCTSIB ( p = .13)); or for months in treatment (SCAT Symptom Score ( p = .23), SCAT Symptom Severity Score ( p = .04), CISS ( p = .41), DHI ( p = .37), mCTSIB ( p = .50)).

Conclusions

Improvements were similar for all patients receiving post-concussive physical therapy, regardless of time between injury and treatment onset, and regardless of time spent in treatment. These results may have implications for clinical decision-making and for third party payors’ coverage of post-concussion treatment. Longer periods of treatment may not necessarily be of greater benefit and application of treatment if delayed may also be beneficial. Limitations to the study, such as its retrospective nature, lack of randomization, and convenience sample size are discussed.
Digital Technologies for Emotion-Regulation Assessment and Intervention: A Conceptual Review
Authors: Bettis Alexandra H. , Burke Taylor A. , Nesi Jacqueline , Liu Richard T.
Published: June 2021

The ability to regulate emotions in response to stress is central to healthy development. Whereas early research in emotion regulation predominantly employed static, self-report measurement, the past decade has seen a shift in focus toward understanding the dynamic nature of regulation processes. This is reflected in recent refinements in the definition of emotion regulation that emphasize the importance of the ability to flexibly adapt regulation efforts across contexts. The latest proliferation of digital technologies employed in mental health research offers the opportunity to capture the state- and context-sensitive nature of emotion regulation. In this conceptual review, we examine the use of digital technologies (ecological momentary assessment; wearable and smartphone technology, physical activity, acoustic data, visual data, and geo-location; smart-home technology; virtual reality; social media) in the assessment of emotion regulation and describe their application to interventions. We also discuss challenges and ethical considerations and outline areas for future research.
Megalithic Period in North Maidan, Karnataka
Authors: Thakur Hema
Published: June 2021

The megalithic period in Karnataka is significant as it witnessed technological developments like the beginning of use of iron and fast wheel for the making of pottery as well as socio-economic and institutional developments such as the emergence of an agrarian base, demographic expansion, rudimentary craft specialisation, a developing exchange network and a degree of social differentiation. Many features typical of the megalithic phase continue into the early historic period. In fact, the megalithic and the early historical do not essentially represent two distinct chronological entities and are found overlapping at certain sites. So far, there is no literary evidence for the culture, and there is nothing to suggest that the society was literate—if graffiti on the megalithic pottery do not turn out to be symbols of writing
Book review: Zak Cope, The Wealth of (Some) Nations—Imperialism and the Mechanics of Value Transfer
Authors: Habib Irfan
Published: June 2021

Zak Cope, The Wealth of (Some) Nations—Imperialism and the Mechanics of Value Transfer (London: Pluto Press), 2019, 260 pp., £19.99 (Pb).
Book review: Suvobrata Sarkar, Let There Be Light: Engineering, Entrepreneurship and Electricity in Colonial Bengal, 1880–1945
Authors: Sinha J.N.
Published: June 2021

Suvobrata Sarkar, Let There Be Light: Engineering, Entrepreneurship and Electricity in Colonial Bengal, 1880–1945 (New Delhi: Cambridge University Press), 2020, xiv + 294 pp., (hb.), ₹659.
Fishing for Feedback: Discussing Individual Competencies and Strengths in Teams
Authors: Abdon Winters Susan , Spataro Sandra E. , Hughes Stephanie
Published: June 2021

Management students conduct much of their coursework in teams, as they should. We teach them where teams can fail and how teams can become high performing. But too often, the team experience for students is solely about logistics and coordination. Students often miss the rich benefits of using their self-awareness and social intelligence to develop their abilities to give and receive deep peer feedback. This article introduces a playful format for teams to gain experience giving meaningful, lasting, individualized feedback for every team member. The exercise is appropriate for teams from undergraduates to executives and can be part of any course where students work in teams.
Stroke and splenic infarct in a 17-year-old patient with COVID-associated hypercoagulable state and relative ADAMTS13 deficiency
Authors: Pistor Maximilian , Hoffmann Angelika , Horvath Thomas , Oberholzer Michael , Arnold Marcel , Pilgram-Pastor Sara Magdalena
Published: June 2021

In this case report, we present the case of a 17-year-old stroke patient suffering from coronavirus disease (COVID)-19. He was successfully treated with intravenous and endovascular treatment. After extensive work-up, a hypercoagulable state due to the COVID-19 infection was assumed as probable cause of stroke.
Advance care planning among older Canadians amid the COVID-19 pandemic: a focus on sexual orientation
Authors: de Vries Brian , Gutman Gloria , Beringer Robert , Gill Paneet , Daudt Helena
Published: June 2021

Objectives:

The COVID-19 pandemic has led to death and hardship around the world, and increased popular discourse about end-of-life circumstances and conditions. The extent to which this discourse and related pandemic experiences have precipitated advance care planning (ACP) activities was the focus of this study with a particular emphasis on sexual orientation.

Methods:

A large, national online survey was conducted between 10 August and 10 October 2020 in Canada. The final sample of 3923 persons aged 55 and older was recruited using social media, direct email, and Facebook advertising and in conjunction with community groups. Women comprised almost 78% of the sample; just more than 7% of the sample identified as lesbian, gay, and bisexual (LGB). Measures included demographic variables and a series of questions on ACP, including documents and discussions undertaken prior to the pandemic and since its onset.

Results:

Descriptive analyses revealed few gender or sexual orientation differences on documents and discussions prior to the pandemic; since its onset, LGB persons have completed or initiated wills, powers of attorney, advance directives, representation agreements, and have engaged in ACP discussions in greater proportion than heterosexuals. Logistic regressions reveal the increased likelihood of pre-pandemic ACP engagement by age, gender (women), and education; since the pandemic onset, gender, education, and sexual orientation were predictive of greater ACP engagement. Care discussions were more likely undertaken by women and LGB persons since the pandemic most often with spouses, family, and friends, especially among LGB persons.

Discussion:

Gender roles and previous pandemic experiences (HIV/AIDS, in particular) are implicated in this pattern of results; opportunities for educational interventions are considered.
An Interview on District and School Transformation: Practical Approaches to Facilitating Sustainable Systemic Change
Authors: Schneider Kurt A. , Sands Suzanne , Endre Kristen , Baker Dale , Burnette Kristin , Ryndak Diane
Published: June 2021

Illinois has an education system that includes regional cooperatives charged with supporting member districts in the provision of special education services for students from birth to age 22. Such support can include both direct services (i.e., early childhood services, special education classes in a separate building or in special education classrooms of the member districts) and indirect services (e.g., consulting services, professional development and coaching, assessment, related services). This interview is with three leaders in one such cooperative of 18 K-8 and high school member districts of varying size across 3 regions in 2 counties.
Understanding Shared Responsibility Between Special and General Education Teachers in the Rural Classroom
Authors: Berry Ann B.
Published: June 2021

A shared sense of responsibility for the education of students with disabilities can have positive effects on both teachers and students. When special education (SE) and general education (GE) teachers work together, this collaborative relationship is a positive variable in teacher satisfaction and retention. Furthermore, teacher collaboration and an inclusive delivery of SE services in the GE classroom can foster increased student achievement, motivation, self-esteem, and social growth. In this study, the researcher conducted individually administered surveys with 35 teachers in two rural districts to further explore the variable of a shared sense of responsibility: what tasks, how, and where responsibilities were shared. Following the presentation of the survey results is a description of the professional development that was provided to teachers in collaboration and co-teaching. Barriers and benefits to increasing teacher collaboration and co-teaching are discussed, including outcomes for students in one co-taught classroom over a 2-year period.
Biologicals in childhood severe asthma: the European PERMEABLE survey on thestatus quo
Authors: Santos-Valente Elisangela , Buntrock-Döpke Heike , Abou Taam Rola , Arasi Stefania , Bakirtas Arzu , Lozano Blasco Jaime , Bønnelykke Klaus , Craiu Mihai , Cutrera Renato , Deschildre Antoine , Elnazir Basil , Fleming Louise , Frey Urs , Gappa Monika , Nieto García Antonio , Skamstrup Hansen Kirsten , Hanssens Laurence , Jahnz-Rozyk Karina , Jesenak Milos , Kerzel Sebastian , Kopp Matthias V. , Koppelman Gerard H. , Krivec Uros , MacLeod Kenneth A. , Mäkelä Mika , Melén Erik , Mezei Györgyi , Moeller Alexander , Moreira Andre , Pohunek Petr , Minić Predrag , Rutjes Niels W.P. , Sammut Patrick , Schwerk Nicolaus , Szépfalusi Zsolt , Turkalj Mirjana , Tzotcheva Iren , Ulmeanu Alexandru , Verhulst Stijn , Xepapadaki Paraskevi , Niggel Jakob , Vijverberg Susanne , Maitland-van der Zee Anke H. , Potočnik Uroš , Reinartz Susanne M. , van Drunen Cornelis M. , Kabesch Michael
Published: June 2021

Introduction

Severe asthma is a rare disease in children, for which three biologicals, anti-immunoglobulin E, anti-interleukin-5 and anti-IL4RA antibodies, are available in European countries. While global guidelines exist on who should receive biologicals, knowledge is lacking on how those guidelines are implemented in real life and which unmet needs exist in the field. In this survey, we aimed to investigate thestatus quoand identify open questions in biological therapy of childhood asthma across Europe.

Methods

Structured interviews regarding experience with biologicals, regulations on access to the different treatment options, drug selection, therapy success and discontinuation of therapy were performed. Content analysis was used to analyse data.

Results

We interviewed 37 experts from 25 European countries and Turkey and found a considerable range in the number of children treated with biologicals per centre. All participating countries provide public access to at least one biological. Most countries allow different medical disciplines to prescribe biologicals to children with asthma, and only a few restrict therapy to specialised centres. We observed significant variation in the time point at which treatment success is assessed, in therapy duration and in the success rate of discontinuation. Most participating centres intend to apply a personalised medicine approach in the future to match patientsa priorito available biologicals.

Conclusion

Substantial differences exist in the management of childhood severe asthma across Europe, and the need for further studies on biomarkers supporting selection of biologicals, on criteria to assess therapy response and on how/when to end therapy in stable patients is evident.
Dysbiosis of the gut microbiome is a risk factor for osteoarthritis in older female adults: a case control study
Authors: Chen Juanjuan , Wang Anqi , Wang Qi
Published: June 2021

Background

Osteoarthritis (OA) is a multifactorial joint degenerative disease with low-grade inflammation. The gut microbiome has recently emerged as an pathogenic factor of OA, and prebiotics supplementation could alleviate OA symptoms in animal models. However, the relationship between the gut microbiome and OA in the older female adults is hitherto not clear.

Results

Here we studied the gut microbiome of 57 OA patients and their healthy controls by metagenome-wide association study based on previously published data. A significant reduction in the richness and diversity of gut microbiome were observed in OA patients. Bifidobacterium longum and Faecalibacterium prausnitzii were decreased while Clostridium spp. was increased in the OA group. The functional modules, particularly the energetic metabolism and acetate production were also decreased in the OA patients. To evaluate the diagnostic value of identified species for elderly patients with OA, we constructed a set of random forest disease classifiers based on species differences between the two groups. Among them, 9 species reached the lowest classification error in the random forest cross validation, and the area under ROC of the model was 0.81.

Conclusions

Significant alterations in the gut microbial composition and function were observed between the older patients with OA and their controls, and a random forest classifier model for OA were constructed based on the differences in our study. Our study have identified several potential gut microbial targets in the elderly females with OA, which will facilitate the treatment of OA based on gut microbiota, is of great value in alleviating pain and improving the quality of life for them.
FEGS: a novel feature extraction model for protein sequences and its applications
Authors: Mu Zengchao , Yu Ting , Liu Xiaoping , Zheng Hongyu , Wei Leyi , Liu Juntao
Published: June 2021

Background

Feature extraction of protein sequences is widely used in various research areas related to protein analysis, such as protein similarity analysis and prediction of protein functions or interactions.

Results

In this study, we introduce FEGS (Feature Extraction based on Graphical and Statistical features), a novel feature extraction model of protein sequences, by developing a new technique for graphical representation of protein sequences based on the physicochemical properties of amino acids and effectively employing the statistical features of protein sequences. By fusing the graphical and statistical features, FEGS transforms a protein sequence into a 578-dimensional numerical vector. When FEGS is applied to phylogenetic analysis on five protein sequence data sets, its performance is notably better than all of the other compared methods.

Conclusion

The FEGS method is carefully designed, which is practically powerful for extracting features of protein sequences. The current version of FEGS is developed to be user-friendly and is expected to play a crucial role in the related studies of protein sequence analyses.
Historical genomics reveals the evolutionary mechanisms behind multiple outbreaks of the host-specific coffee wilt pathogen Fusarium xylarioides
Authors: Peck Lily D. , Nowell Reuben W. , Flood Julie , Ryan Matthew J. , Barraclough Timothy G.
Published: June 2021

Background

Nearly 50% of crop yields are lost to pests and disease, with plants and pathogens locked in an amplified co-evolutionary process of disease outbreaks. Coffee wilt disease, caused by Fusarium xylarioides, decimated coffee production in west and central Africa following its initial outbreak in the 1920s. After successful management, it later re-emerged and by the 2000s comprised two separate epidemics on arabica coffee in Ethiopia and robusta coffee in east and central Africa.

Results

Here, we use genome sequencing of six historical culture collection strains spanning 52 years to identify the evolutionary processes behind these repeated outbreaks. Phylogenomic reconstruction using 13,782 single copy orthologs shows that the robusta population arose from the initial outbreak, whilst the arabica population is a divergent sister clade to the other strains. A screen for putative effector genes involved in pathogenesis shows that the populations have diverged in gene content and sequence mainly by vertical processes within lineages. However, 15 putative effector genes show evidence of horizontal acquisition, with close homology to genes from F. oxysporum. Most occupy small regions of homology within wider scaffolds, whereas a cluster of four genes occupy a 20Kb scaffold with strong homology to a region on a mobile pathogenicity chromosome in F. oxysporum that houses known effector genes. Lacking a match to the whole mobile chromosome, we nonetheless found close associations with DNA transposons, especially the miniature impala type previously proposed to facilitate horizontal transfer of pathogenicity genes in F. oxysporum. These findings support a working hypothesis that the arabica and robusta populations partly acquired distinct effector genes via transposition-mediated horizontal transfer from F. oxysporum, which shares coffee as a host and lives on other plants intercropped with coffee.

Conclusion

Our results show how historical genomics can help reveal mechanisms that allow fungal pathogens to keep pace with our efforts to resist them. Our list of putative effector genes identifies possible future targets for fungal control. In turn, knowledge of horizontal transfer mechanisms and putative donor taxa might help to design future intercropping strategies that minimize the risk of transfer of effector genes between closely-related Fusarium taxa.
FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
Authors: Miao Yingjing , Cao Jiashu , Huang Li , Yu Youjian , Lin Sue
Published: June 2021

Background

As an important subfamily of arabinogalactan proteins (AGPs), fasciclin-like AGPs (FLAs) contribute to various aspects of growth, development and adaptation, yet their function remains largely elusive. Despite the diversity of FLAs, only two members, Arabidopsis FLA3 and rice MTR1, are reported to be involved in sexual reproduction. In this study, another Arabidopsis FLA-encoding gene, FLA14, was identified, and its role was investigated.

Results

Arabidopsis FLA14 was found to be a pollen grain-specific gene. Expression results from fusion with green fluorescent protein showed that FLA14 was localized along the cell membrane and in Hechtian strands. A loss-of-function mutant of FLA14 showed no discernible defects during male gametogenesis, but precocious pollen germination occurred inside the mature anthers under high moisture conditions. Overexpression of FLA14 caused 39.2% abnormal pollen grains with a shrunken and withered appearance, leading to largely reduced fertility with short mature siliques and lower seed set. Cytological and ultramicroscopic observation showed that ectopic expression of FLA14 caused disruption at the uninucleate stage, resulting in either collapsed pollen with absent intine or pollen of normal appearance but with a thickened intine.

Conclusions

Taken together, our data suggest a role for FLA14 in pollen development and preventing premature pollen germination inside the anthers under high relative humidity in Arabidopsis.
Feasibility and postoperative opioid sparing effect of an opioid-free anaesthesia in adult cardiac surgery: a retrospective study
Authors: Aguerreche Clément , Cadier Gaspard , Beurton Antoine , Imbault Julien , Leuillet Sébastien , Remy Alain , Zaouter Cédrick , Ouattara Alexandre
Published: June 2021

Background

No previous study investigated the dexmedetomidine-based opioid-free anesthesia (OFA) protocol in cardiac surgery. The main objective of this study was to evaluate the feasibility and the postoperative opioid-sparing effect of dexmedetomidine-based OFA in adult cardiac surgery patients.

Methods

We conducted a single-centre and retrospective study including 80 patients above 18 years old who underwent on-pump cardiac surgery between November 2018 and February 2020. Patients were divided into two groups: OFA (lidocaine, ketamine, dexmedetomidine, MgSO4) or opioid-based anaesthesia (remifentanil and anti-hyperalgesic medications such as ketamine and/or MgSO4 and/or lidocaine at the discretion of the anesthesiologist). The primary endpoint was the total amount of opioid consumed in its equivalent of intravenous morphine during the first 48 postoperative hours. Secondary outcomes included perioperative hemodynamics, post-operative maximal pain at rest and during coughing and adverse outcomes. Data are expressed as median [interquartile range].

Results

Patients in the OFA-group had a higher EuroSCORE II, with more diabetes, more dyslipidemia and more non-elective surgery but fewer smoking history. In the OFA group, the median loading dose of dexmedetomidine was 0.6 [0.4–0.6] μg.kg− 1 while the median maintenance dose was 0.11 μg.kg− 1.h− 1 [0.05–0.20]. In 10 (25%) patients, dexmedetomidine was discontinued for a drop of mean arterial pressure below 55 mmHg. The median total amount of opioid consumed in its equivalent of intravenous morphine during the first 48 postoperative hours was lower in the OFA group (15.0 mg [8.5–23.5] versus 30.0 mg [17.3–44.3], p < 0.001). While no differences were seen with rest pain (2.0 [0.0–3.0] versus 0.5 [0.0–5.0], p = 0.60), the maximal pain score during coughing was lower in OFA group (3.5 [2.0–5.0] versus 5.5 [3.0–7.0], p = 0.04). In OFA group the incidence of atrial fibrillation (18% versus 40%, p = 0.03) and non-invasive ventilation use (25% versus 48%, p = 0.04) were lower. The incidence of bradycardia and the intraoperative use of norepinephrine were similar between both groups.

Conclusion

Dexmedetomidine-based OFA in cardiac surgery patients is feasible and could be associated with a lower postoperative morphine consumption and better postoperative outcomes. Further randomized studies are required to confirm these promising results and determine the optimal associations, dosages, and infusion protocols during cardiac surgery.

Graphical abstract
Future directions of Doctor of Public Health education in the United States: a qualitative study
Authors: Park Chulwoo , Migliaccio Gene , Edberg Mark , Frehywot Seble , Johnson Geralyn
Published: June 2021

Background

The Doctor of Public Health (DrPH) degree is an advanced and terminal professional degree that prepares the future workforce to engage in public health research, teaching, practice, and leadership. The purpose of the present research was to discuss the desirable future direction and optimal education strategies for the DrPH degree in the United States.

Methods

A total of 28 Council on Education for Public Health (CEPH)-accredited DrPH programs in the United States was identified through the Association of Schools and Programs of Public Health (ASPPH) Academic Program Finder. Then, a qualitative analysis was conducted to obtain perspectives from a total of 20 DrPH program directors through in-depth interviews.

Results

A DrPH program should be recognized as equal but different from an MPH or a PhD program and strengthen the curriculum of methodology and leadership education. It is important that a DrPH program establishes specific partnerships with other entities and provide funding for students. In addition, rather than being standardized nationwide, there is value in each DrPH program maintaining its unique character and enabling students to be open to all career pathways.

Conclusions

The future of DrPH programs in the twenty-first century should aim at effective interdisciplinary public health approaches that draw from the best of both academic and applied sectors. A DrPH program is expected to provide academic, applied public health, and leadership training for students to pursue careers in either academia or the public/private sector, because public health is an applied social science that bridges the gap between research and practice.
A cross-sectional study measuring contact patterns using diaries in an urban and a rural community in South Africa, 2018
Authors: Kleynhans Jackie , Tempia Stefano , McMorrow Meredith L. , von Gottberg Anne , Martinson Neil A. , Kahn Kathleen , Moyes Jocelyn , Mkhencele Thulisa , Lebina Limakatso , Gómez-Olivé F. Xavier , Wafawanaka Floidy , Mathunjwa Azwifarwi , Cohen Cheryl ,   the PHIRST group, Buys Amelia , Mathee Angela , Language Brigitte , Maake Lorens , Treurnicht Florette , Mothlaoleng Katlego , Carrim Maimuna , Wolter Nicole , Hellferscee Orienka , Wagner Ryan G. , Piketh Stuart
Published: June 2021

Background

Describing contact patterns is crucial to understanding infectious disease transmission dynamics and guiding targeted transmission mitigation interventions. Data on contact patterns in Africa, especially South Africa, are limited. We measured and compared contact patterns in a rural and urban community, South Africa. We assessed participant and contact characteristics associated with differences in contact rates.

Methods

We conducted a cross-sectional study nested in a prospective household cohort study. We interviewed participants to collect information on persons in contact with for one day. We described self-reported contact rates as median number people contacted per day, assessed differences in contact rates based on participant characteristics using quantile regression, and used a Poisson model to assess differences in contact rates based on contact characteristics within age groups. We also calculated cumulative person hours in contact within age groups at different locations.

Results

We conducted 535 interviews (269 rural, 266 urban), with 17,252 contacts reported. The overall contact rate was 14 (interquartile range (IQR) 9–33) contacts per day. Those ≤18 years had higher contact rates at the rural site (coefficient 17, 95% confidence interval (95%CI) 10–23) compared to the urban site, for those aged 14–18 years (13, 95%CI 3–23) compared to < 7 years. No differences were observed for adults. There was a strong age-based mixing, with age groups interacting more with similar age groups, but also interaction of participants of all ages with adults. Children aged 14–18 years had the highest cumulative person hours in contact (116.3 rural and 76.4 urban).

Conclusions

Age played an important role in the number and duration of contact events, with children at the rural site having almost double the contact rate compared to the urban site. These contact rates can be utilized in mathematical models to assess transmission dynamics of infectious diseases in similar communities.