bluuebunny/crossref_metadata_2025 huggingface dataset:
Oxidation of Benzylic Alcohols and Lignin Model Compounds with Layered Double Hydroxide Catalysts
Authors: Mobley Justin K. , Jennings John A. , Morgan Tonya , Kiefer Axel , Crocker Mark
Published: August 2018

Alcohol oxidation to carbonyl compounds is one of the most commonly used reactions in synthetic chemistry. Herein, we report the use of base metal layered double hydroxide (LDH) catalysts for the oxidation of benzylic alcohols in polar solvents. These catalysts are ideal reagents for alcohol oxidations due to their ease of synthesis, tunability, and ease of separation from the reaction medium. LDHs synthesized in this study were fully characterized by means of X-ray diffraction, NH3-temperature programmed desorption (TPD), pulsed CO2 chemisorption, N2 physisorption, electron microscopy, and elemental analysis. LDHs were found to effectively oxidize benzylic alcohols to their corresponding carbonyl compounds in diphenyl ether, using O2 as the terminal oxidant. LDH catalysts were also applied to the oxidation of lignin β-O-4 model compounds. Typically, for all catalysts, only trace amounts of the ketone formed from benzylic alcohol oxidation were observed, the main products comprising benzoic acids and phenols arising from β-aryl ether cleavage. This observation is consistent with the higher reactivity of the ketones, resulting from weakening of the Cβ–O4 bond that was shown to be aerobically cleaved at 180 °C in the absence of a catalyst.
Targeted Molecular Treatments in Non-Small Cell Lung Cancer: A Clinical Guide for Oncologists
Authors: Bui Kim Tam , Cooper Wendy A. , Kao Steven , Boyer Michael
Published: August 2018

Targeted molecular treatments have changed the way non-small cell lung cancer (NSCLC) is managed. Epidermal growth factor receptor (EGFR), anaplastic lymphoma kinase (ALK), v-raf murine sarcoma viral oncogene homolog B1 (BRAF), and c-ros oncogene 1 (ROS1) mutations are now used to guide specific anti-cancer therapies to improve patient outcomes. New targeted molecular treatments are constantly being developed and evaluated as a means to improve efficacy, overcome resistance, or minimise toxicity. This review article summarises the current evidence for the efficacy, resistance mechanisms, and safety of targeted molecular treatments against specific mutations in NSCLC.
A New Measure for Assessing Substance-Related and Addictive Disorders: The Addictive Behavior Questionnaire (ABQ)
Authors: Caretti Vincenzo , Gori Alessio , Craparo Giuseppe , Giannini Marco , Iraci-Sareri Giuseppe , Schimmenti Adriano
Published: August 2018

This article evaluates the psychometric properties of a new measure for assessing Substance-Related and Addictive Disorders: the Addictive Behavior Questionnaire (ABQ). The ABQ is a self-report measure composed of two sections: the Severity Index (SI) and the Seven Domains Addiction Scale (7DAS). Materials and methods. A total sample of 698 subjects divided into two groups (515 subjects in the clinical sample and 183 subjects in the control sample), participated in this study. We applied Exploratory Factor Analysis (EFA) to examine features of ABQ construct validity, we used Cronbach’s alpha coefficient to assess its internal reliability, and explored some aspects of its concurrent validity by examining its associations with other measures assessing addictive behaviors and psychopathology. Results and conclusions: results of EFA indicated that all the scales of the ABQ are unidimensional and showed good internal consistency. The correlations between the sections of the ABQ and the other measures used in the current study were significant and in the expected directions. These results suggest that the ABQ has good psychometric properties and allows researchers and clinicians to gather relevant information regarding behaviors, psychopathology and severity of symptoms, for the best clinical reasoning and for planning tailored treatment for each patient.
Effects of Meditative Movements on Major Depressive Disorder: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Zou Liye , Yeung Albert , Li Chunxiao , Wei Gao-Xia , Chen Kevin W. , Kinser Patricia Anne , Chan Jessie S. M. , Ren Zhanbing
Published: August 2018

Background: Tai Chi, Qigong, and Yoga are recognized as the most popular complementary approaches for alleviating musculoskeletal pain, improving sleep quality, and reducing blood pressure. The therapeutic effects of these meditative movements for treating major depressive disorder (MDD) is yet to be determined. Therefore, we examined whether meditative movements (Tai Chi, Qigong, and Yoga) are effective for treating MDD. Seven electronic databases (SPORTDiscus, PubMed, PsycINFO, Cochrane Library, Web of Science, CNKI, and Wanfang) were used to search relevant articles. Randomized controlled trials (RCT) using Tai Chi, Qigong or Yoga as intervention for MDD were considered for the meta-analysis (standardized mean difference: SMD). Results: Meta-analysis on 15 fair-to-high quality RCTs showed a significant benefit in favor of meditative movement on depression severity (SMD = −0.56, 95% CI −0.76 to −0.37, p < 0.001, I2 = 35.76%) and on anxiety severity (SMD = −0.46, 95% CI −0.71 to −0.21, p < 0.001, I2 = 1.17%). Meditative movement interventions showed significantly improved treatment remission rate (OR = 6.7, 95% CI 2.38 to 18.86, p < 0.001) and response rate (OR = 5.2, 95% CI 1.73 to 15.59, p < 0.001) over passive controls. Conclusions: Emphasizing the therapeutic effects of meditative movements for treating MDD is critical because it may provide a useful alternative to existing mainstream treatments (drug therapy and psychotherapy) for MDD. Given the fact that meditative movements are safe and easily accessible, clinicians may consider recommending meditative movements for symptomatic management in this population.
Incorporating Germanium Oxide into the Glass Phase of Novel Zinc/Magnesium-Based GPCs Designed for Bone Void Filling: Evaluating Their Physical and Mechanical Properties
Authors: Khader Basel A. , Rodriguez Omar , Towler Mark R.
Published: August 2018

The structural role of Germanium (Ge), when substituting for Zinc (Zn) up to 8 mol % in the 0.48SiO2–0.12CaO–0.36ZnO–0.04MgO glass series, was investigated with respect to both the glass chemistry and also the properties of glass polyalkenoate cements (GPCs) manufactured from them. The Network connectivity (NC) of the glass was calculated to increase from 1.83 to 2.42 with the addition of GeO2 (0–8 mol %). Differential thermal analysis (DTA) results confirmed an increase in the glass transition temperature (Tg) of the glass series with GeO2 content. X-ray photoelectron spectroscopy (XPS) showed an increase in the ratio of bridging oxygens (BO) to non-bridging oxygens (NBO) with the addition of GeO2, supporting the NC and DTA results. 29Si magic angle spinning nuclear magnetic resonance spectroscopy (29Si MAS-NMR) determined a chemical shift from −80.3 to −83.7 ppm as the GeO2 concentration increased. These ionomeric glasses were subsequently used as the basic components in a series of GPCs by mixing them with aqueous polyacrylic acid (PAA). The handling properties of the GPCs resulting were evaluated with respect to the increasing concentration of GeO2 in the glass phase. It was found that the working times of these GPCs increased from 3 to 15 min, while their setting times increased from 4 to 18 min, facilitating the injectability of the Zn/Mg-GPCs through a 16-gauge needle. These Ge-Zn/Mg-GPCs were found to be injectable up to 96% within 12 min. Zn/Mg-GPCs containing GeO2 show promise as injectable cements for use in bone void filling.
A Comparative Study of Two State-of-the-Art Feature Selection Algorithms for Texture-Based Pixel-Labeling Task of Ancient Documents
Authors: Mehri Maroua , Chaieb Ramzi , Kalti Karim , Héroux Pierre , Mullot Rémy , Essoukri Ben Amara Najoua
Published: August 2018

Recently, texture features have been widely used for historical document image analysis. However, few studies have focused exclusively on feature selection algorithms for historical document image analysis. Indeed, an important need has emerged to use a feature selection algorithm in data mining and machine learning tasks, since it helps to reduce the data dimensionality and to increase the algorithm performance such as a pixel classification algorithm. Therefore, in this paper we propose a comparative study of two conventional feature selection algorithms, genetic algorithm and ReliefF algorithm, using a classical pixel-labeling scheme based on analyzing and selecting texture features. The two assessed feature selection algorithms in this study have been applied on a training set of the HBR dataset in order to deduce the most selected texture features of each analyzed texture-based feature set. The evaluated feature sets in this study consist of numerous state-of-the-art texture features (Tamura, local binary patterns, gray-level run-length matrix, auto-correlation function, gray-level co-occurrence matrix, Gabor filters, Three-level Haar wavelet transform, three-level wavelet transform using 3-tap Daubechies filter and three-level wavelet transform using 4-tap Daubechies filter). In our experiments, a public corpus of historical document images provided in the context of the historical book recognition contest (HBR2013 dataset: PRImA, Salford, UK) has been used. Qualitative and numerical experiments are given in this study in order to provide a set of comprehensive guidelines on the strengths and the weaknesses of each assessed feature selection algorithm according to the used texture feature set.
Evaluating the Performance of Structure from Motion Pipelines
Authors: Bianco Simone , Ciocca Gianluigi , Marelli Davide
Published: August 2018

Structure from Motion (SfM) is a pipeline that allows three-dimensional reconstruction starting from a collection of images. A typical SfM pipeline comprises different processing steps each of which tackles a different problem in the reconstruction pipeline. Each step can exploit different algorithms to solve the problem at hand and thus many different SfM pipelines can be built. How to choose the SfM pipeline best suited for a given task is an important question. In this paper we report a comparison of different state-of-the-art SfM pipelines in terms of their ability to reconstruct different scenes. We also propose an evaluation procedure that stresses the SfM pipelines using real dataset acquired with high-end devices as well as realistic synthetic dataset. To this end, we created a plug-in module for the Blender software to support the creation of synthetic datasets and the evaluation of the SfM pipeline. The use of synthetic data allows us to easily have arbitrarily large and diverse datasets with, in theory, infinitely precise ground truth. Our evaluation procedure considers both the reconstruction errors as well as the estimation errors of the camera poses used in the reconstruction.
Immunopathogenesis of Human Sporotrichosis: What We Already Know
Authors: Conceição-Silva Fatima , Morgado Fernanda Nazaré
Published: August 2018

Sporotrichosis is a subacute/chronic mycosis caused by dimorphic fungus of the genus Sporothrix. This mycosis may affect both human and domestic animals and in the last few years, the geographic dispersion and increase of sporotrichosis worldwide has been observed. The occurrence of cases related to scratching/bites of domestic felines have increased, characterizing the disease as predominantly a zoonosis. In humans, sporotrichosis mainly involves the cutaneous tegument of infected patients, but other tissues may also present the infection. The main forms of clinical presentation are lymphocutanous sporotrichosis (LC) and fixed sporotrichosis (F). Although less common, mucosal, cutaneous disseminated, and extracutaneous forms have also been described. Multiple factors from the fungus and host can play a role in driving the clinical evolution of sporotrichosis to benign or severe disease. In this review, we discuss the immunopathological aspects involved in human sporotrichosis. Putting together the two branches of knowledge—host immune response and fungal evading mechanisms—we may perceive new possibilities in understanding the fungus–host interaction in order to be in a position to go further in the control of sporotrichosis.
Therapy of Mucormycosis
Authors: Sipsas Nikolaos V. , Gamaletsou Maria N. , Anastasopoulou Amalia , Kontoyiannis Dimitrios P.
Published: August 2018

Despite the recent introduction of mold-active agents (posaconazole and isavuconazole), in addition to amphotericin B products, to our armamentarium against mucormycosis, many uncertainties remain for the management of this uncommon opportunistic infection, as there are no data from prospective randomized clinical trials to guide therapy. In this mini-review, we present the current status of treatment options. In view of the heterogeneity of the disease (different types of affected hosts, sites of infection, and infecting Mucorales), mucormycosis management requires an individualized management plan that takes into account the net state of immunosuppression of the host, including comorbidities, certainty of diagnosis, site of infection, and antifungal pharmacological properties.
Do Better Political Institutions Help in Reducing Political Pressure on State-Owned Banks? Evidence from Developing Countries
Authors: Ashraf Badar , Arshad Sidra , Yan Liang
Published: August 2018

This study examines whether state-owned banks face political pressure and whether the improvement in political institutions alleviates this pressure. The theory of political benefits argues that politicians use state-owned banks for political purposes such as obtaining and maintaining political support. We reviewed extant empirical research and found that the existing evidence is mixed; some studies support while others reject the theory. In this backdrop, we analyzed a sample of 185 state-owned banks from 51 developing countries over the period 1998–2012 and provide renewed evidence supporting the theory. Specifically, we found that state-owned banks face significant political pressure in developing countries; that is, they lend more and earn less in election years. Next, we observed that the political pressure is prevalent only in the countries with weak political institutions. Strong political institutions in the form of higher constraints on policy change decisions of incumbent government and higher democratic accountability are helpful in eliminating political pressure on state-owned banks in developing countries.
Revisiting (Non-)Native Influence in VOT Production: Insights from Advanced L3 Spanish
Authors: Llama Raquel , Cardoso Walcir
Published: August 2018

A growing body of research investigating cross-linguistic influence on the acquisition of a third phonological system suggests that first (L1) and second (L2) languages concur in influencing oral production in the target third language (L3). Yet, there are also claims of either a more noticeable effect of the L2 on the L3, or a prevailing influence from the L1. This study further explores whether the L1 and the L2 compete or converge on exerting influence on L3 pronunciation. To do so, we examine the production of voice onset time for voiceless stops by adult advanced learners of L3 Spanish divided into two groups (15 L1 English-L2 French, and 15 L1 French-L2 English speakers). Three monolingual control groups were also tested. Participants were recorded reading word lists that contained voiceless stops in stressed onset position. A Kruskal-Wallis test uncovered significant differences traceable to the L1-English speakers, which puts them at a slight disadvantage vis-à-vis their Francophone counterparts. These results favor claims of a more decisive role for the L1 in L3 pronunciation. We compare our results to findings from previous studies targeting intermediate learners, and find proficiency in the L3 may account for the observed differences.
Influence of PLA Filament Conditions on Characteristics of FDM Parts
Authors: Valerga Ana Pilar , Batista Moisés , Salguero Jorge , Girot Frank
Published: August 2018

Additive manufacturing technologies play an important role in Industry 4.0. One of the most prevalent processes is fused deposition modelling (FDM) due to its versatility and low cost. However, there is still a lack of standardization of materials and procedures within this technology. This work aims to study the relationship of certain operating parameters and the conditions of poly(lactic acid) (PLA) polymer with the results of the manufactured parts in dimensional terms, surface quality, and mechanical strength. In this way, the impact of some material characteristics is analyzed, such as the pigmentation of the material and the environmental humidity where it has been stored. The manufacturing parameter that relates to these properties has been the extrusion temperature since it is the most influential in this technology. The results are quite affected especially by humidity, being a parameter little studied in the literature.
Applicability of Mechanical Tests for Biomass Pellet Characterisation for Bioenergy Applications
Authors: Williams Orla , Taylor Simon , Lester Edward , Kingman Sam , Giddings Donald , Eastwick Carol
Published: August 2018

In this paper, the applicability of mechanical tests for biomass pellet characterisation was investigated. Pellet durability, quasi-static (low strain rate), and dynamic (high strain rate) mechanical tests were applied to mixed wood, eucalyptus, sunflower, miscanthus, and steam exploded and microwaved pellets, and compared to their Hardgrove Grindability Index (HGI), and milling energies for knife and ring-roller mills. The dynamic mechanical response of biomass pellets was obtained using a novel application of the Split Hopkinson pressure bar. Similar mechanical properties were obtained for all pellets, apart from steam-exploded pellets, which were significantly higher. The quasi-static rigidity (Young’s modulus) was highest in the axial orientation and lowest in flexure. The dynamic mechanical strength and rigidity were highest in the diametral orientation. Pellet strength was found to be greater at high strain rates. The diametral Young’s Modulus was virtually identical at low and high strain rates for eucalyptus, mixed wood, sunflower, and microwave pellets, while the axial Young’s Modulus was lower at high strain rates. Correlations were derived between the milling energy in knife and ring roller mills for pellet durability, and quasi-static and dynamic pellet strength. Pellet durability and diametral quasi-static strain was correlated with HGI. In summary, pellet durability and mechanical tests at low and high strain rates can provide an indication of how a pellet will break down in a mill.
Biocompatible/Biodegradable Electrowetting on Dielectric Microfluidic Chips with Fluorinated CTA/PLGA
Authors: Zhang Kaidi , Chao Lei , Zhou Jia
Published: August 2018

One of the major hurdles in the development of biocompatible/biodegradable EWOD (Electrowetting-on-dielectric) devices is the biocompatibility of the dielectric and hydrophobic layers. In this study, we address this problem by using reactive ion etching (RIE) to prepare a super-hydrophobic film combining fluorinated cellulose triacetate (CTA) and poly (lactic-co-glycolic acid) (PLGA). The contact angle (CA) of water droplets on the proposed material is about 160°. X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) characterizations indicate that a slight increase in the surface roughness and the formation of CFx (C-F or CF2) bonds are responsible for the super-hydrophobic nature of the film. Alternating Current (AC) static electrowetting and droplet transportation experiments evidence that contact angle hysteresis and contact line pinning are greatly reduced by impregnating the CTA/PLGA film with silicon oil. Therefore, this improved film could provide a biocompatible alternative to the typical Teflon® or Cytop® films as a dielectric and hydrophobic layer.
The Influence of Manufacturing Parameters on the Mechanical Behaviour of PLA and ABS Pieces Manufactured by FDM: A Comparative Analysis
Authors: Rodríguez-Panes Adrián , Claver Juan , Camacho Ana María
Published: August 2018

This paper presents a comparative study of the tensile mechanical behaviour of pieces produced using the Fused Deposition Modelling (FDM) additive manufacturing technique with respect to the two types of thermoplastic material most widely used in this technique: polylactide (PLA) and acrylonitrile butadiene styrene (ABS). The aim of this study is to compare the effect of layer height, infill density, and layer orientation on the mechanical performance of PLA and ABS test specimens. The variables under study here are tensile yield stress, tensile strength, nominal strain at break, and modulus of elasticity. The results obtained with ABS show a lower variability than those obtained with PLA. In general, the infill percentage is the manufacturing parameter of greatest influence on the results, although the effect is more noticeable in PLA than in ABS. The test specimens manufactured using PLA perform more rigidly and they are found to have greater tensile strength than ABS. The bond between layers in PLA turns out to be extremely strong and is, therefore, highly suitable for use in additive technologies. The methodology proposed is a reference of interest in studies involving the determination of mechanical properties of polymer materials manufactured using these technologies.
Effect of Strain Heterogeneities on Microstructure, Texture, Hardness, and H-Activation of High-Pressure Torsion Mg Consolidated from Different Powders
Authors: Panda Subrata , Toth Laszlo S. , Zou Jianxin , Grosdidier Thierry
Published: August 2018

Severe plastic deformation techniques, such as high-pressure torsion (HPT), have been increasingly applied on powder materials to consolidate bulk nanostructured materials. In this context, the aim of the present study is to compare the plastic deformation characteristics during HPT of two distinct Mg-based powder precursors: (i) atomized micro-sized powder and (ii) condensed and passivated nanopowder. Dynamic recrystallization could take place during HPT consolidation of the atomized powder particles while the oxide pinning of the grain boundaries restricted it for the condensed powder. Consequently, there have been substantial differences in the development of the microstructure, texture, local strain heterogeneities, and hardness in the two types of consolidated products. Different types of local strain heterogeneities were also revealed in the consolidated products. The associated diversity in microstructure within the same consolidated product has been demonstrated to have an effect on the hydrogen activation kinetics to form hydrides for these Mg-based materials that could be suitable for solid state H-storage applications.
An Empirical Investigation on a Multiple Filters-Based Approach for Remaining Useful Life Prediction
Authors: Trinh Hung-Cuong , Kwon Yung-Keun
Published: August 2018

Feature construction is critical in data-driven remaining useful life (RUL) prediction of machinery systems, and most previous studies have attempted to find a best single-filter method. However, there is no best single filter that is appropriate for all machinery systems. In this work, we devise a straightforward but efficient approach for RUL prediction by combining multiple filters and then reducing the dimension through principal component analysis. We apply multilayer perceptron and random forest methods to learn the underlying model. We compare our approach with traditional single-filtering approaches using two benchmark datasets. The former approach is significantly better than the latter in terms of a scoring function with a penalty for late prediction. In particular, we note that selecting a best single filter over the training set is not efficient because of overfitting. Taken together, we validate that our multiple filters-based approach can be a robust solution for RUL prediction of various machinery systems.
Influence of Monovalent Cations on the Efficiency of Ferrous Ion Oxidation, Total Iron Precipitation, and Adsorptive Removal of Cr(VI) and As(III) in Simulated Acid Mine Drainage with Inoculation of Acidithiobacillus ferrooxidans
Authors: Song Yongwei , Wang Heru , Yang Jun , Cao Yanxiao
Published: August 2018

Acid mine drainage is highly acidic and contains large quantities of Fe and heavy metal elements. Thus, it is important to promote the transformation of Fe into secondary iron minerals that exhibit strong heavy-metal removal abilities. Using simulated acid mine drainage, this work analyzes the influence of monovalent cations (K+, NH4+, and Na+) on the Fe2+ oxidation and total Fe deposition efficiencies, as well as the phases of secondary iron minerals in an Acidithiobacillus ferrooxidans system. It also compares the Cr(VI) (K2Cr2O7) and As(III) (As2O3) removal efficiencies of different schwertmannites. The results indicated that high concentrations of monovalent cations (NH4+ ≥ 320 mmol/L, and Na+ ≥ 1600 mmol/L) inhibited the biological oxidation of Fe2+. Moreover, the mineralizing abilities of the three cations differed (K+ > NH4+ > Na+), with cumulative Fe deposition efficiencies of 58.7%, 28.1%, and 18.6%, respectively [n(M) = 53.3 mmol/L, cultivation time = 96 h]. Additionally, at initial Cr(VI) and As(III) concentrations of 10 and 1 mg/L, respectively, the Cr(VI) and As(III) removal efficiencies exhibited by schwertmannites acquired by the three mineralization systems differed [n(Na) = 53.3 > n(NH4) = 53.3 > n(K) = 0.8 mmol/L]. Overall, the analytical results suggested that the removal efficiency of toxic elements was mainly influenced by the apparent structure, particle size, and specific surface area of schwertmannite.
Experimental and Numerical Investigations of a Novel Laser Impact Liquid Flexible Microforming Process
Authors: Liu Fei , Liu Huixia , Jiang Chenkun , Ma Youjuan , Wang Xiao
Published: August 2018

A novel high strain rate microforming technique, laser impact liquid flexible embossing (LILFE), which uses laser induced shock waves as an energy source, and liquid as a force transmission medium, is proposed by this paper in order to emboss three-dimensional large area micro arrays on metallic foils and to overcome some of the defects of laser direct shock microembossing technology. The influences of laser energy and workpiece thickness on the deformation characteristics of the pure copper foils with the LILFE process were investigated through experiments and numerical simulation. A finite element model was built to further understand the typical stages of deformation, and the results of the numerical simulation are consistent with those achieved from the experiments. The experimental and simulation results show that the forming accuracy and depth of the embossed parts increases with the increase in laser energy and decrease in workpiece thickness. The thickness thinning rate of the embossed parts increases with the decrease of the workpiece thickness, and the severest thickness thinning occurs at the bar corner region. The experimental results also show that the LILFE process can protect the workpiece surface from being ablated and damaged, and can ensure the surface quality of the formed parts. Besides, the numerical simulation studies reveal the plastic strain distribution of embossed microfeatures under different laser energy.
Isolation and Purification of Potent Growth Inhibitors from Piper methysticum Root
Authors: Van Truong Mai , Xuan Tran Dang , Minh Truong Ngoc , Quan Nguyen Van
Published: August 2018

Piper methysticum (kava) root is known to possess promising weed suppressing activity. The present study was conducted to search for potent plant growth inhibitors from the root of this medicinal pepper plant. The ethyl acetate (EtOAc) extract exhibited the strongest reduction on growth of Raphanus sativus (radish) (IC50 shoot and root growth = 172.00 and 51.31 µg/mL respectively) among solvent extracts. From this active extract, nine potent growth inhibitors involved in the inhibitory activities of P. methysticum root were isolated, purified and characterized by column chromatography (CC), gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). The six fractions purified by CC included two flavanones: 5-hydroxy-4′,7-dimethoxyflavanone (C1) and 5,7-dihydroxy-4′-methoxy-6,8-dimethylflavanone (matteucinol, C2) and six kavalactones: 5,6-dehydro-kavain (C3), a mixture of kavain and yagonin (C4), yagonin (C5) and dihydro-5,6-dehydrokavain, 7,8-dihydrokavain, dihydromethysticin and methysticin (C6). The amounts of 5-hydroxy-4′,7-dimethoxyflavanone, matteucinol, 5,6-dehydrokavain and yangonin were 0.76, 2.50, 2.75 and 2.09 mg/g dry weight (DW), respectively. The two flavanones C1 and C2 exhibited the strongest inhibition on shoot elongation (IC50 = 120.22 and 248.03 µg/mL, respectively), whilst the two kavalactone mixtures C4 and C6 showed the highest suppression on root growth of R. sativus (IC50 = 7.70 and 15.67 µg/mL, respectively). This study was the first to report the purification and inhibitory activities of the two flavanones 5-hydroxy-4′,7-dimethoxyflavanone and matteucinol in P. methysticum root. The isolated constituents from P. methysticum root including the flavanones C1 and C2 and the mixtures C4 and C6 may possess distinct modes of action on plant growth. Findings of this study highlighted that the combinations of hexane-ethyl acetate by 9:1 and 8:2 ratios successfully purified flavanones and kavalactones in P. methysticum root.
Physicochemical Characterization, Antioxidant and Immunostimulatory Activities of Sulfated Polysaccharides Extracted from Ascophyllum nodosum
Authors: Chen Ligen , Wang Yan , Yang Hui , Li Han , Xu Wei , Chen Guijie , Zhu Hongjun
Published: August 2018

Polysaccharides from Ascophyllum nodosum (AnPS) were extracted and purified via an optimized protocol. The optimal extraction conditions were as follows: extraction time of 4.3 h, extraction temperature of 84 °C and ratio (v/w, mL/g) of extraction solvent (water) to raw material of 27. The resulting yield was 9.15 ± 0.23% of crude AnPS. Two fractions, named AnP1-1 and AnP2-1 with molecular weights of 165.92 KDa and 370.68 KDa, were separated from the crude AnPS by chromatography in DEAE Sepharose Fast Flow and Sephacryl S-300, respectively. AnP1-1 was composed of mannose, ribose, glucuronic acid, glucose and fucose, and AnP2-1 was composed of mannose, glucuronic acid, galactose and fucose. AnPS, AnP1-1 and AnP2-1 exhibited high scavenging activities against ABTS radical and superoxide radical, and showed protective effect on H2O2-induced oxidative injury in RAW264.7 cells. Furthermore, the immunostimulatory activities of AnP1-1 and AnP2-1 were evaluated by Caco-2 cells, the results showed both AnP1-1 and AnP2-1 could significantly promote the production of immune reactive molecules such as interleukin (IL)-8, IL-1β, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. Therefore, the results suggest that AnPS and its two fractions may be explored as a potential functional food supplement.
Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion
Authors: Giampieri Francesca , Afrin Sadia , Stewart Derek , McDougall Gordon J. , Brennan Rex , Blyth Lesley , Gasparrini Massimiliano , Mazzoni Luca , Capocasa Franco , Alvarez-Suarez Josè Miguel , Bompadre Stefano , Nogueira Brás de Oliveira Pedro , N. Santos Claudia , Masias Manuel , Agudo Pablo , Crespo Jorge , Mezzetti Bruno , Forbes-Hernández Tamara Y. , Battino Maurizio
Published: August 2018

Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
Transition-Metal-Free C(sp3)–H Oxidation of Diarylmethanes
Authors: Yang Fan , Zhou Bihui , Chen Pu , Zou Dong , Luo Qiannan , Ren Wenzhe , Li Linlin , Fan Limei , Li Jie
Published: August 2018

An efficient direct C(sp3)–H oxidation of diarylmethanes has been demonstrated by this study. This method employs environment-friendly O2 as an oxidant and is promoted by commercially available MN(SiMe3)2 [M = K, Na or Li], which provides a facile method for the synthesis of various diaryl ketones in excellent yields. This protocol is metal-free, mild and compatible with a number of functional groups on substrates.
High-Throughput Genotyping of CRISPR/Cas Edited Cells in 96-Well Plates
Authors: Nussbaum Lea , Telenius Jelena M. , Hill Stephanie , Hirschfeld Priscila P. , Suciu Maria C. ,   The WIGWAM Consortium, Downes Damien J. , Hughes Jim R.
Published: August 2018

The emergence in recent years of DNA editing technologies—Zinc finger nucleases (ZFNs), transcription activator-like effector (TALE) guided nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/Cas family enzymes, and Base-Editors—have greatly increased our ability to generate hundreds of edited cells carrying an array of alleles, including single-nucleotide substitutions. However, the infrequency of homology-dependent repair (HDR) in generating these substitutions in general requires the screening of large numbers of edited cells to isolate the sequence change of interest. Here we present a high-throughput method for the amplification and barcoding of edited loci in a 96-well plate format. After barcoding, plates are indexed as pools which permits multiplexed sequencing of hundreds of clones simultaneously. This protocol works at high success rate with more than 94% of clones successfully genotyped following analysis.
Control of the Alumina Microstructure to Reduce Gate Leaks in Diamond MOSFETs
Authors: Gutiérrez Marina , Lloret Fernando , Pham Toan T. , Cañas Jesús , Reyes Daniel F. , Eon David , Pernot Julien , Araújo Daniel
Published: August 2018

In contrast to Si technology, amorphous alumina cannot act as a barrier for a carrier at diamond MOSFET gates due to their comparable bandgap. Indeed, gate leaks are generally observed in diamond/alumina gates. A control of the alumina crystallinity and its lattice matching to diamond is here demonstrated to avoid such leaks. Transmission electron microscopy analysis shows that high temperature atomic layer deposition, followed by annealing, generates monocrystalline reconstruction of the gate layer with an optimum lattice orientation with respect to the underneath diamond lattice. Despite the generation of γ-alumina, such lattice control is shown to prohibit the carrier transfer at interfaces and across the oxide.
Solid Organ Transplant and Parasitic Diseases: A Review of the Clinical Cases in the Last Two Decades
Authors: Fabiani Silvia , Fortunato Simona , Bruschi Fabrizio
Published: August 2018

The aim of this study was to evaluate the occurrence of parasitic infections in solid organ transplant (SOT) recipients. We conducted a systematic review of literature records on post-transplant parasitic infections, published from 1996 to 2016 and available on PubMed database, focusing only on parasitic infections acquired after SOT. The methods and findings of the present review have been presented based on the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) checklist. From data published in the literature, the real burden of parasitic infections among SOT recipients cannot really be estimated. Nevertheless, publications on the matter are on the increase, probably due to more than one reason: (i) the increasing number of patients transplanted and then treated with immunosuppressive agents; (ii) the “population shift” resulting from immigration and travels to endemic areas, and (iii) the increased attention directed to diagnosis/notification/publication of cases. Considering parasitic infections as emerging and potentially serious in their evolution, additional strategies for the prevention, careful screening and follow-up, with a high level of awareness, identification, and pre-emptive therapy are needed in transplant recipients.
An Opportunity for Pharmacists to Help Improve Coordination and Continuity of Patient Health Care
Authors: Schommer Jon C. , Brown Lawrence M. , Bortz Ryan (Alyssa) , Cernasev Alina , Gomaa Basma T. , Hager Keri D. , Hillman Lisa , Okoro Olihe , Pakhomov Serguei V. S. , Ranelli Paul L.
Published: August 2018

Pharmacist workforce researchers are predicting a potential surplus of pharmacists in the United States that might result in pharmacists being available for engagement in new roles. The objective for this study was to describe consumer opinions regarding medication use, the health care system, and pharmacists to help identify new roles for pharmacists from the consumer perspective. Data were obtained from the 2015 and 2016 National Consumer Surveys on the Medication Experience and Pharmacist Roles. Out of the representative sample of 36,673 respondents living in the United States, 80% (29,426) submitted written comments at the end of the survey. Of these, 2178 were specifically about medicines, pharmacists or health and were relevant and usable for this study. Thematic analysis, content analysis, and computer-based text mining were used for identifying themes and coding comments. The findings showed that 66% of the comments about medication use and 82% about the health care system were negative. Regarding pharmacists, 73% of the comments were positive with many commenting about the value of the pharmacist for overcoming fears and for filling current gaps in their healthcare. We propose that these comments might be signals that pharmacists could help improve coordination and continuity for peoples’ healthcare and could help guide the development of new service offerings.
Identity of Low-Molecular-Weight Species Formed in End-To-End Cyclization Reactions Performed in THF
Authors: Pan Ching W. , Xia Katherine , Parker Samantha A. , Tillman Eric S.
Published: August 2018

Cyclic polymers were produced by end-to-end coupling of telechelic linear polymers under dilute conditions in THF, using intramolecular atom transfer radical coupling or click chemistry. In addition to the expected shift to longer elution times on gel permeation chromatography (GPC) indicative of the formation of cyclic product, lower molecular weight species were consistently observed upon analysis of the unpurified cyclization reaction mixture. By systematically removing or altering single reaction components in the highly dilute cyclization reaction, it was found that THF itself was responsible for the low-molecular-weight material, forming oligomeric chains of poly(THF) regardless of the other reaction components. When the reactions were performed at higher concentrations and for shorter time intervals, conducive to intermolecular chain-end-joining reactions, the low-molecular-weight peaks were absent. Isolation of the material and analysis by 1H NMR confirmed that poly(THF) was being formed in the highly dilute conditions required for cyclization by end-to-end coupling. When a series of mock cyclization reactions were performed with molar ratios of the reactants held constant, but concentrations changed, it was found that lower concentrations of reactants led to higher amounts of poly(THF) side product.
Regional Diversity in the Postsynaptic Proteome of the Mouse Brain
Authors: Roy Marcia , Sorokina Oksana , McLean Colin , Tapia-González Silvia , DeFelipe Javier , Armstrong J. Douglas , Grant Seth G. N.
Published: August 2018

The proteome of the postsynaptic terminal of excitatory synapses comprises over one thousand proteins in vertebrate species and plays a central role in behavior and brain disease. The brain is organized into anatomically distinct regions and whether the synapse proteome differs across these regions is poorly understood. Postsynaptic proteomes were isolated from seven forebrain and hindbrain regions in mice and their composition determined using proteomic mass spectrometry. Seventy-four percent of proteins showed differential expression and each region displayed a unique compositional signature. These signatures correlated with the anatomical divisions of the brain and their embryological origins. Biochemical pathways controlling plasticity and disease, protein interaction networks and individual proteins involved with cognition all showed differential regional expression. Combining proteomic and connectomic data shows that interconnected regions have specific proteome signatures. Diversity in synapse proteome composition is key feature of mouse and human brain structure.
Implications of Land-Grabbing on the Ecological Balance of Brazil
Authors: Coscieme Luca , Niccolucci Valentina , Giannetti Biagio F. , Pulselli Federico M. , Marchettini Nadia , Sutton Paul C.
Published: August 2018

In the global free-market, natural resource scarcity and opportunities for preserving the local environment are fostering international purchasing of large extensions of land, mainly for agricultural use. These land transactions often involve land cover change (i.e., through deforestation) or a shift from extensive or traditional to intensive agricultural practices. In Brazil, the land appropriation by foreign investors (i.e., the so-called “land-grabbing”) is affecting natural capital availability for local communities to a different extent in the very different territorial entities. At the same time, Brazilian investors are purchasing land in other countries. Ecological footprint accounting is one appropriate lens that can be employed to visualize the aggregated effect of natural capital appropriation and use. The aim of this paper is to provide a first estimate on the effect of land-grabbing on the ecological balance of Brazil through calculating the biocapacity embodied in purchased lands in the different states of Brazil. The results show that Brazil is losing between 9 to 9.3 million global hectares (on a gross basis, or a net total of 7.7 to 8.6 million of global hectares) of its biocapacity due to land-grabbing, when considering respectively a “cropland to cropland” (i.e., no land-cover change) and a “total deforestation” scenario. This represents a minimum estimate, highlighting the need for further land-grabbing data collection at the subnational scale. This analysis can be replicated for other countries of the world, adjusting their ecological balance by considering the biocapacity embodied in international transactions of land.
Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry
Authors: Welsh Joshua A. , Kepley Julia , Rosner Ariel , Horak Peter , Berzofsky Jay A. , Jones Jennifer C.
Published: August 2018

Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry.
Detection of Talking in Respiratory Signals: A Feasibility Study Using Machine Learning and Wearable Textile-Based Sensors
Authors: Ejupi Andreas , Menon Carlo
Published: August 2018

Social isolation and loneliness are major health concerns in young and older people. Traditional approaches to monitor the level of social interaction rely on self-reports. The goal of this study was to investigate if wearable textile-based sensors can be used to accurately detect if the user is talking as a future indicator of social interaction. In a laboratory study, fifteen healthy young participants were asked to talk while performing daily activities such as sitting, standing and walking. It is known that the breathing pattern differs significantly between normal and speech breathing (i.e., talking). We integrated resistive stretch sensors into wearable elastic bands, with a future integration into clothing in mind, to record the expansion and contraction of the chest and abdomen while breathing. We developed an algorithm incorporating machine learning and evaluated its performance in distinguishing between periods of talking and non-talking. In an intra-subject analysis, our algorithm detected talking with an average accuracy of 85%. The highest accuracy of 88% was achieved during sitting and the lowest accuracy of 80.6% during walking. Complete segments of talking were correctly identified with 96% accuracy. From the evaluated machine learning algorithms, the random forest classifier performed best on our dataset. We demonstrate that wearable textile-based sensors in combination with machine learning can be used to detect when the user is talking. In the future, this approach may be used as an indicator of social interaction to prevent social isolation and loneliness.
A Solar Panel-Integrated Modified Planner Inverted F Antenna for Low Earth Orbit Remote Sensing Nanosatellite Communication System
Authors: Alam Touhidul , Islam Mohammad Tariqul , Ullah Md. Amanath , Cho Mengu
Published: August 2018

One of the most efficient methods to observe the impact of geographical, environmental, and geological changes is remote sensing. Nowadays, nanosatellites are being used to observe climate change using remote sensing technology. Communication between a remote sensing nanosatellite and Earth significantly depends upon antenna systems. Body-mounted solar panels are the main source of satellite operating power unless deployable solar panels are used. Lower ultra-high frequency (UHF) nanosatellite antenna design is a crucial challenge due to the physical size constraint and the need for solar panel integration. Moreover, nanosatellite space missions are vulnerable because of antenna and solar panel deployment complexity. This paper proposes a solar panel-integrated modified planner inverted F antenna (PIFA) to mitigate these crucial limitations. The antenna consists of a slotted rectangular radiating patch with coaxial probe feeding and a rectangular ground plane. The proposed antenna has achieved a −10 dB impedance bandwidth of 6.0 MHz (447.5 MHz–453.5 MHz) with a small-sized (80 mm× 90 mm× 0.5 mm) radiating element. In addition, the antenna achieved a maximum realized gain of 0.6 dB and a total efficiency of 67.45% with the nanosatellite structure and a solar panel. The challenges addressed by the proposed antenna are to ensure solar panel placement between the radiating element and the ground plane, and provide approximately 55% open space to allow solar irradiance into the solar panel.