bluuebunny/crossref_metadata_2025 huggingface dataset:
Agrobiodiversity as a Reservoir of Medicinal Resources: Ethnobotanical Insights from Aymara Communities in the Bolivian Andean Altiplano
Authors: Cocarico Simón , Rivera Diego , Beck Stephan , Obón Concepción
Published: January 2025

This study investigates the medicinal potential of cultivated plants and weeds in Aymara communities around Lake Titicaca. It highlights the intricate connection between horticultural diversity and traditional healing practices. Through ethnobotanical research involving 228 informants across multiple locations in Bolivia, we documented 239 medicinal plant species, focusing on the diversity within cultivated landscapes. Among these, 56 species are cultivated crops, 17 are agricultural weeds, and 19 species have dual status, serving as both wild and cultivated plants depending on environmental conditions. Women are repositories of knowledge for 81% (193) of total medicinal plant species, while men know 47% (113) of species. Women display dominant knowledge of cultivated species (89%, or 50 species) and purchase medicinal plants from local markets; women know 92% (24 species) vs. men’s 15% (4 species). Our results suggest men may use a smaller set of species more frequently, while women know and use a broader range of species. The analysis of plant life forms revealed the significant medicinal roles of perennials, annuals, subshrubs, and shrubs, which together account for over 67% of the documented species and 73% of the use reports. Arboreal species are present but have a comparatively smaller role in traditional medicine. In total, we recorded 1477 use reports addressing 260 pathologies across 28 major health categories. Notably, cultivated plants and weeds represent 38.8% of the medicinal species, highlighting their essential role in local healthcare practices. By assessing the contributions of native and introduced species, this study sheds light on the complex botanical resources integrated into Aymara agricultural systems. These findings deepen our understanding of medicinal plant diversity and underscore the importance of agrobiodiversity as a cornerstone of community health and cultural resilience in the Andean region. This research also emphasizes the often-overlooked medicinal value of cultivated landscapes and agricultural margins.
Climatic Factors Influencing Aleppo Pine Sap Flow in Orographic Valleys Under Two Contrasting Mediterranean Climates
Authors: Sabater Ana M. , Valiente José Antonio , Bellot Juan , Vilagrosa Alberto
Published: January 2025

Global climate change projections highlight the Mediterranean Basin as one of the most susceptible areas to the effects of intense and prolonged droughts, as well as increasing air temperatures. Accordingly, the productivity and survival of forests in this area will depend on their ability to resist and adapt to increasingly drier conditions. Different climatic conditions across the Mediterranean Basin could drive differences in forest functioning, requiring trees to acclimate to them. Sea breeze dynamics along orographic valleys can also influence climatic conditions, accentuating differences between inland and coastal forests. However, there is limited information on whether the climatic factors regulating tree transpiration in Aleppo pine forest in orographic valleys vary according to climate. This study aims to identify and compare the climatic factors that regulate tree transpiration along a gradient and determine the thresholds at which these factors affect transpiration rates. This study was carried out by means of sap flow gauges, since this technique is a key feature for quantifying and understanding tree transpiration. It was conducted in two Aleppo pine dry sub-humid forests (inland and coastal, 750 and 675 trees ha−1, respectively) and in two pine semi-arid forests (inland and coastal, 600 and 400 trees ha−1, respectively) in the western Mediterranean basin during January–November of 2021. No significant rainfall events or droughts were recorded during the period of study, indicating a standard climatic condition in these areas. The main findings demonstrated that the variability in sap flow could be attributed to the interaction between soil water content and vapour pressure deficit in all the forests studied. However, the highest threshold values of these climatic factors in relation to the increase or decrease in maximum sap flow (i.e., less sensitivity) were exhibited in semi-arid forests, highlighting the adaptability of Aleppo pine to more limiting climatic conditions. These findings are relevant for the consequences of the predicted increase in harsh climatic conditions and the balance among vapour pressure deficit, temperature and soil water availability. Future research will be essential to confirm forest acclimatisation in the transitional dry to semi-arid forest ecosystems predicted by global climate change projections, given their potential to strongly alter ecosystem function and water cycles.
Psychosocial Climate as Antecedent for Resources to Manage Emotional Demands at Work
Authors: Andersen Lars Peter , Andersen Dorte Ruby , Pihl-Thingvad Jesper
Published: January 2025

Background: Effectively managing emotional demands in the workplace is crucial in professions such as healthcare, education, and social work. Recent studies indicate that the psychosocial safety climate within an organization can significantly mitigate the negative effects associated with high emotional demands. Method: This study examines whether psychosocial safety climate is associated with six practical strategies for managing emotional demands that have previously been found to be associated with less burnout. It utilizes longitudinal data from 1457 participants across 129 different workplaces in various emotionally demanding professions. The associations were analyzed using linear regression and Poisson regression techniques. All analyses incorporated cluster-robust standard errors to account for potential dependencies arising from respondents sharing the same workplace. Results: The results indicate that higher levels of psychosocial safety climate are significantly associated with the availability of practical strategies. Furthermore, the incidence rate of receiving multiple types of support increases significantly for each point increase in psychosocial safety. Additionally, these relationships remain consistent even under high work demands. Conclusions: The results underscore the importance of fostering a psychosocial safety climate and increasing the availability of practical strategies to manage emotional demands in the workplace. This approach may safeguard employee well-being in high-demand professions.
Chemoprotective Mechanism of Sodium Thiosulfate Against Cisplatin-Induced Nephrotoxicity Is via Renal Hydrogen Sulfide, Arginine/cAMP and NO/cGMP Signaling Pathways
Authors: Dugbartey George J. , Alornyo Karl K. , Adams Ismaila , Adjei Samuel , Amoah Daniel , Obeng-Kyeremeh Richard
Published: January 2025

Cisplatin is a common and highly effective chemotherapeutic agent whose nephrotoxic side effect is well-characterized. Sodium thiosulfate (STS), an FDA-approved hydrogen sulfide (H2S) donor drug, is emerging as a chemoprotective agent against cisplatin-induced nephrotoxicity (CIN). In this study, we investigated the chemoprotective mechanism of STS in a rat model of CIN. Twenty-five male Sprague Dawley rats were randomly assigned to the following groups: HC: Healthy control (received 10 mL/kg/day of 0.9% saline intraperitoneally (ip), [n = 5]), CIN: Cisplatin (received single dose of 7 mg/kg cisplatin ip [n = 5]); CIN + PAG: Cisplatin and daily ip administration of 40 mg/kg of the H2S inhibitor, DL-propargylglycine (PAG) for 28 days (n = 5); CIN + PAG + STS: Cisplatin and daily PAG and STS (150 µM) ip injection for 28 days; CIN + STS: Cisplatin and daily STS ip administration for 28 days (n = 5). Rats in each group were kept in metabolic cages for 24 h on day 0, 14 and 29 after cisplatin administration for urine collection. Rats were then euthanized, and kidney and blood samples were collected for analysis. Histologically, CIN was characterized by glomerular and tubular injury and significant macrophage influx and tubular apoptosis, as well as markedly increased levels of plasma and renal IL-1β, IL-6 and TNF-α and impaired renal antioxidant status compared to HC rats (p < 0.001). These pathological changes were exacerbated in CIN + PAG rats and were strongly reduced in CIN + PAG + STS rats relative to CIN + PAG rats (p < 0.01), while superior renal protection was observed in CIN + STS rats. Functionally, CIN was evidenced by markedly increased levels of serum creatinine and BUN, and significantly decreased urine creatinine, renal creatinine clearance, as well as electrolyte imbalance and urinary concentrating defect in comparison with HC (p < 0.01). These functional changes worsened significantly in CIN + PAG rats (p < 0.05) but improved in CIN + PAG + STS rats, with further improvement in CIN + STS rats to levels comparable to HC rats. Mechanistically, STS increased renal and plasma levels of H2S, arginine, cAMP, nitric oxide (NO) and cGMP as well as SIRT3 and PGC-1α. We have shown for the first time that STS provides chemoprotection against CIN by activating renal arginine/cAMP and NO/cGMP signaling pathways and their downstream mechanisms through increased renal H2S production.
A Low-Modulus Phosphatidylserine-Exposing Microvesicle Alleviates Skin Inflammation via Persistent Blockade of M1 Macrophage Polarization
Authors: Zhang Zihao , Mo Yidi , Xu Shengxia , Jiang Lei , Peng Yuanshu , ZhuGe Yani , Su Zhijian , Xiang Qi , Zeng Rong , Zhang Guanglin
Published: January 2025

Inflammatory skin diseases comprise a group of skin conditions characterized by damage to skin function due to overactive immune responses. These disorders not only impair the barrier function of the skin but also deteriorate the quality of life and increase the risk of psychiatric issues. Here, a low-modulus phosphatidylserine-exposing microvesicle (deformed PSV, D-PSV) was produced, characterized, and evaluated for its potential therapeutic function against skin diseases. Compared to conventional PSVs (C-PSVs), D-PSVs exhibited a more robust and longer-lasting inhibitory effect on the inflammatory response triggered by lipopolysaccharides and interferon-γ in a primary bone marrow-derived macrophage model. Transcriptome analysis indicated that the inhibitory effect of D-PSVs was mainly achieved by modulating inflammation-related signaling pathways, leading to a reduction in the expressions of pro-inflammatory genes. In an imiquimod-induced psoriatic dermatitis mouse model, topical application of D-PSVs effectively mitigated inflammation in the skin microenvironment and reduced lesion severity. These improvements were attributed to the superior skin permeability and more persistent adhesion of D-PSVs to macrophages compared with C-PSVs. In summary, this macrophage-targeted microvesicle offers a promising non-invasive approach to managing inflammatory skin diseases by persistently inhibiting M1 macrophage polarization and restoring immune microenvironment balance.
Mechanism of THBS1 Regulation of MDCK Cell Proliferation and Apoptosis Through TGF-β/Smad Signalling
Authors: Li Rui , Zhang Fan , Wang Lijin , Wang Siya , Zhou Manlin , Wang Jun , Zhang Yiyang , Tan Xiao , Chen Weiji , Yang Kun , Qiao Zilin
Published: January 2025

Madin–Darby Canine Kidney (MDCK) cells are a key cell line for influenza vaccine production, due to their high viral yield and low mutation resistance. In our laboratory, we established a tertiary cell bank (called M60) using a standard MDCK cell line imported from American Type Culture Collection (ATCC) in the USA. Due to their controversial tumourigenicity, we domesticated non-tumourigenic MDCK cells (named CL23) for influenza vaccine production via monoclonal screening in the early stage of this study, and the screened CL23 cells were characterised based on their low proliferative capacity, which had certain limitations in terms of expanding their production during cell resuscitation. It was thus our objective to enhance the proliferation efficiency of MDCK cells for influenza vaccine production following cell resuscitation, with a view to improving the production of non-tumourigenic MDCK cells for vaccines and enhancing the production of influenza virus lysate vaccines from MDCK cells through genetic intervention. We concentrated on the protein thrombosponin-1 (THBS1), which was markedly differentiated in the proteomics data of the two MDCK cells. By integrating this finding with related studies, we were able to ascertain that THBS1 exerts a significant influence on the level of cell proliferation and apoptosis. Consequently, our objective was to investigate the impact of THBS1 expression on MDCK cell apoptosis by verifying the differences in THBS1 expression between the two MDCK cells and by interfering with THBS1 expression in the MDCK cells. We found that the knockdown of THBS1 significantly increased the proliferation and apoptosis of CL23 cells without causing significant changes in cell migration and invasion, and its overexpression significantly decreased the proliferation of M60 cells and increased cell migration, invasion, and apoptosis. In addition, the TGF-β/Smad pathway target genes transforming growth factor-β1 (TGF-β1), mothers against decapentaplegic homolog 2 (Smad2), and mothers against decapentaplegic homolog 3 (Smad3), were significantly down-regulated in CL23 cells after THBS1 knockdown and up-regulated in M60 cells after overexpression, with consistent expression identified at both the mRNA and protein levels. The treatment of cells with TGF-β activators and inhibitors further demonstrated that THBS1 regulated MDCK cell proliferation and apoptosis through the TGF-β/Smad signalling pathway. Finally, we found that THBS1 also regulated H1N1 influenza virus replication. These findings enable a comprehensive understanding of the regulatory mechanisms of THBS1 regarding MDCK cell proliferation and apoptosis functions and the effects of influenza virus replication.
A New Strategy in Modulating the Protease-Activated Receptor 2 (Par2) in Autoimmune Diseases
Authors: Khoon Lynn , Piran Ron
Published: January 2025

Autoimmune diseases are complex conditions characterized by immune-mediated tissue damage and chronic inflammation. Protease-activated receptor 2 (Par2) has been implicated in these diseases, exhibiting dual roles that complicate its therapeutic potential. This review examines the perplexing functions of Par2, which promotes inflammation through immune cell activation while facilitating tissue healing in damaged organs. By analyzing findings across diverse autoimmune conditions, including rheumatoid arthritis, type 1 diabetes, and inflammatory bowel disease, we highlight how the context and location of Par2 activation determine its effects. Recent studies from our laboratory have resolved some of these contradictions by distinguishing Par2’s immune-mediated inflammatory roles from its tissue-reparative functions. These insights pave the way for context-specific therapeutic strategies, such as selective Par2 modulators, that can mitigate inflammation while enhancing tissue repair. However, achieving such precision in modulation remains a significant challenge, necessitating further research into Par2’s signaling pathways. This review underscores Par2’s complexity and its transformative potential in autoimmune disease management, offering a nuanced perspective on its duality and therapeutic implications.
Correction: Tian et al. Genome-Wide Identification of the DnaJ Gene Family in Citrus and Functional Characterization of ClDJC24 in Response to Citrus Huanglongbing. Int. J. Mol. Sci. 2024, 25, 11967
Authors: Tian Yuzhen , Wang Xizi , Huang Huoqing , Deng Xin , Zhang Baihong , Meng Yixuan , Wu Libo , Chen Hang , Zhong Yun , Chen Wenli
Published: January 2025

In the original publication [...]
The Role of Exercise-Based Cardiac Rehabilitation After Myocardial Infarction on Cholesterol Transfer to HDL
Authors: Nicolau Jose C. , Dalcoquio Talia F. , Giraldez Roberto R. , Freitas Fatima R. , Nicolau Andre M. , Furtado Remo H. M. , Tavoni Thauany M. , Baracioli Luciano M. , Lima Felipe G. , Ferrari Aline G. , Rondon Maria U. P. B. , Salsoso Rocio , Alves Maria J. N. N. , Arantes Flavia B. B. , Santos Mayara A. , Alves Leandro S. , Negrao Carlos E. , Maranhão Raul C.
Published: January 2025

High-density lipoprotein (HDL) is associated with decreased incidence of cardiovascular events, and its functionality also influences prognosis. Exercise is an important tool to improve prognosis in the post-infarction (MI) population, but the role of exercise on HDL functionality is poorly understood. Sixty-two patients with acute MI were randomized in a supervised exercise program for 12–14 weeks (exercise group—EG) or a control group (CG). The main objective of the study was to analyze the role of exercise on esterified cholesterol (EC) and unesterified cholesterol (UC) transfer to HDL. For the total population, the baseline mean rate of EC transfer to HDL was 2.53 ± 0.83 and at the end of follow-up, it was 2.74 ± 0.64 (p = 0.03). The figures for UC were, respectively, 4.08 ± 1.2 and 4.4 ± 1.06 (p = 0.02). The difference (follow-up minus baseline) for EC was 0.15 ± 0.84 for the control group and 0.27 ± 0.69 for the exercise group (p = 0.53); for UC, the figures were 0.28 ± 1.14 and 0.35 ± 0.96 (p = 0.80), respectively, for the control and exercise groups. In post-MI patients, 12–14 weeks of supervised exercise did not improve HDL functionality.
Homeodomain Involvement in Nuclear HOX Protein Homo- and Heterodimerization
Authors: Marchese Damien , Evrard Laetitia , Bergiers Isabelle , Boas Ludovic , Duphénieux Justine , Hermant Maryse , Pringels Tamara , Zeqiri Fisnik , Pirson Marc , Twizere Jean-Claude , Gofflot Françoise , Rezsohazy René , Bridoux Laure
Published: January 2025

HOX genes play essential roles in patterning the anteroposterior axis of animal embryos and in the formation of various organs. In mammals, there are 39 HOX genes organized into four clusters (HOXA–D) located on different chromosomes. In relationship with their orderly arrangement along the chromosomes, these genes show nested expression patterns which imply that embryonic territories co-express multiple HOX genes along the main body axis. Interactomic database entries, as well as a handful of publications, support that some HOX proteins can form homodimers or interact with other HOX proteins. However, the consequences of HOX protein interactions have been poorly investigated and remain largely elusive. In this study, we compiled a repository of all HOX–HOX interactions from available databases, and taking HOXA1, HOXA2, and HOXA5 as examples, we investigated the capacity of HOX proteins to form homo- and heterodimers. We revealed that while the DNA-binding domain, the homeodomain, is not necessary for HOXA1 homodimerization, the nuclear localization of the dimerization is dependent on the homeodomain, particularly the integrity of the third helix of HOXA1. Furthermore, we demonstrated that HOXA1 can influence the localization of HOXA1 when it is deprived of the homeodomain, increasing its abundance in the chromatin-containing fraction. Moreover, HOXA1 nuclear homodimerization occurs independently of the integrity of the hexapeptide and, consequently, of its well-known interactor, the homeodomain protein PBX. These results hint at a potential involvement of dimerization in the complex landscape of HOX regulatory mechanisms.
Evaluating the Influence of Drying Shrinkage on Cracking Resistance of Massive Self-Compacting Concrete with Blast Furnace Slag Using Finite Element Method
Authors: Kipkemboi Benson , Miyazawa Shingo
Published: January 2025

The application of self-compacting concrete in massive structures is still low due to the perceived high cracking tendency associated with high shrinkage and heat of cement hydration. This conclusion is from short-term research work of up to 3 months after casting. The purpose of this study is to evaluate the influence of drying shrinkage on the cracking resistance of massive self-compacting concrete with blast furnace slag using a three-dimensional finite element method of analysis (3D FEM). The restraint stress experiment results of the self-compacting concrete beam under drying conditions are compared with those of similar concrete beam models obtained from 3D FEM analysis. The value of the reduction coefficient for Young’s modulus of elasticity was proposed to consider the effect of stress relaxation due to creep in concrete. By using the proposed value of the reduction coefficient, thermal stress analysis was performed on a 1 m thick massive concrete wall member, and the cracking resistance of self-compacting concrete with different replacement ratios of blast furnace slag is discussed. By using the proposed reduction coefficient to evaluate concrete stress due to drying, the accuracy of thermal stress analysis results can be improved to a marginal error of ±15% from the experiment results. Also, the low-temperature rise caused by the high replacement ratio of blast furnace slag at 50% and 70%, respectively, contributed to improved cracking resistance. Furthermore, the optimum blast furnace slag replacement for better cracking resistance depends on the expected exposure condition of the structure.
Report on the Types and Distribution of Antennal Sensilla in Lygaeidae (Heteroptera: Lygaeoidea) and Their Putative Functions
Authors: Nowińska Agnieszka
Published: January 2025

Antennal sensillar structures respond to a variety of environmental stimuli. In the present study, the antennal sensilla of all three subfamilies of the family Lygaeidae are studied for the first time. This research shows the presence of five main types of sensilla (sensilla trichodea, chaetica, campaniformia, basiconica, and coeloconica). Their comparison to other, previously studied, heteropteran insects and their putative mechano-, chemo-, and thermo-hygroreceptive functions are discussed. The current study shows uniformity in the types of sensilla in all studied species. However, minor differences between the subfamilies, as well as within the subfamily Lygaeinae, are observed.
Single-Stage Microsurgical Clipping of Multiple Intracranial Aneurysms in a Patient with Cerebral Atherosclerosis: A Case Report and Review of Surgical Management
Authors: Toader Corneliu , Serban Matei , Covache-Busuioc Razvan-Adrian , Radoi Mugurel Petrinel , Aljboor Ghaith Saleh Radi , Costin Horia Petre , Ilie Milena-Monica , Popa Andrei Adrian , Gorgan Radu Mircea
Published: January 2025

The management of multiple intracranial aneurysms presents significant clinical challenges, particularly when complicated by underlying conditions such as cerebral atherosclerosis. This case report highlights the successful treatment of a 66-year-old female diagnosed with three intracranial aneurysms located in the right middle cerebral artery (MCA), pericallosal artery, and M2 segment. The patient also had a history of systemic atherosclerosis and right-sided breast cancer, factors that increased the complexity of surgical intervention. The aim of this report is to demonstrate the efficacy of single-stage microsurgical clipping in managing multiple aneurysms with favorable outcomes in a complex patient profile. Methods: The patient underwent right-sided pterional craniotomy for microsurgical clipping of all three aneurysms during a single-stage procedure. Two aneurysms in the MCA were clipped using Yasargil clips, and a third aneurysm located at the bifurcation of the pericallosal artery was also secured with a clip. The procedure was performed under microscopic visualization, with meticulous dissection of the atherosclerotic vessels and careful intraoperative hemostasis. Postoperative care involved proactive perioperative management, including blood pressure control and vigilant neurological monitoring. Results: Postoperative imaging at three months confirmed proper clip placement with no evidence of residual aneurysm filling or ischemic complications. The patient exhibited a full neurological recovery, with no deficits or further complications, highlighting the effectiveness of the surgical approach in managing multiple aneurysms concurrently. Conclusions: This case supports the use of single-stage microsurgical clipping as an effective treatment for patients with multiple intracranial aneurysms, even in the presence of complicating factors such as atherosclerosis. A meticulous surgical technique and perioperative management are critical to achieving favorable outcomes and reducing the risk of delayed ischemia or other postoperative complications.
Semi-Automatic Refinement of Myocardial Segmentations for Better LVNC Detection
Authors: Barón Jaime Rafael , Bernabé Gregorio , González-Férez Pilar , García José Manuel , Casas Guillem , González-Carrillo Josefa
Published: January 2025

Background: Accurate segmentation of the left ventricular myocardium in cardiac MRI is essential for developing reliable deep learning models to diagnose left ventricular non-compaction cardiomyopathy (LVNC). This work focuses on improving the segmentation database used to train these models, enhancing the quality of myocardial segmentation for more precise model training. Methods: We present a semi-automatic framework that refines segmentations through three fundamental approaches: (1) combining neural network outputs with expert-driven corrections, (2) implementing a blob-selection method to correct segmentation errors and neural network hallucinations, and (3) employing a cross-validation process using the baseline U-Net model. Results: Applied to datasets from three hospitals, these methods demonstrate improved segmentation accuracy, with the blob-selection technique boosting the Dice coefficient for the Trabecular Zone by up to 0.06 in certain populations. Conclusions: Our approach enhances the dataset’s quality, providing a more robust foundation for future LVNC diagnostic models.
Approach to Mental Health Through a Frequency Modulated Auditory Intervention: A Controlled and Randomized Clinical Trial
Authors: Estalayo-Gutiérrez Beatriz , Álvarez Pasquín María José , Germain Francisco
Published: January 2025

Objective: The clinical trial Effect of Modulated Auditory Stimulation on Interaural Auditory Perception (NCT0544189) aimed to determine whether an auditory intervention (AI)—“Bérard in 10”—can enhance the effect of standard therapies for people with anxiety and/or depression. Methods: Design: unblinded, randomized, controlled clinical trial. Location: Mejorada del Campo Health Centre, Madrid (Primary Care). Participants: A total of 233 patients selected by systematic sampling and meeting the following selection criteria: age of majority, absence of severe acute pathology or chronic decompensated pathology. They were evaluated with the Goldberg and Hamilton tests and classified into the Emotional Well-Being group (EWB, n = 86) or the Anxiety and/or Depression group (AD, n = 147). Just half of each group received an AI. Intervention: Listening to classical music processed through a frequency modulator (Earducator) to attenuate abnormal frequencies, 30 min per session, two sessions a day for 5 days. Main measurements: Hamilton Tests for Anxiety and Hamilton Test for Depression, at 3 and 6 months. Results: In the analysis by protocol, EWB with AI (n = 14) obtained lower scores in anxiety and depression at 3 and 6 months than EWB without AI (n = 36) (p < 0.05), the effects being large and moderate, respectively; AD with AI (n = 31) had lower scores on anxiety and depression at 3 months and anxiety at 6 months than AD without AI (n = 52) (p < 0.05), the effect being small. No damage reported. Conclusions: The AI “Bérard in 10” significantly prevents the onset of anxiety and depression and somewhat improves the effect of standard treatments in primary care.
Examining Cough’s Role and Relief Strategies in Interstitial Lung Disease
Authors: Lim Chee Yao , Khan Sanam Wasim , Alsibai Tarek , Sathiyamoorthy Gayathri
Published: January 2025

Chronic cough is a distressing and prevalent symptom in interstitial lung disease (ILD), significantly impairing quality of life (QoL) and contributing to disease progression, particularly in idiopathic pulmonary fibrosis (IPF). It is associated with physical discomfort, psychological distress, and social isolation and is often refractory to conventional therapies. The pathophysiology of cough in ILD is complex and multifactorial, involving neural hypersensitivity, structural lung changes, inflammatory processes, and comorbid conditions such as gastroesophageal reflux disease (GERD). Evaluating cough in ILD relies on subjective and objective tools to measure its severity, frequency, and impact on daily life, although standardization of these measures remains challenging. Management strategies span pharmacological interventions, including neuromodulators such as opiates, antifibrotic agents, pharmacologic and surgical GERD treatments, and non-pharmacological approaches like behavioral therapies, cough suppression techniques, and pulmonary rehabilitation and physiotherapy. Emerging treatments, such as P2X3 receptor antagonists and airway hydration therapies, offer promising avenues but require further investigation through robust clinical trials. This review aims to demonstrate the importance of addressing cough in ILD as a significant symptom and present objective and subjective methods of quantifying coughs, while providing insights into effective and emerging therapeutic options. By highlighting these potential therapies, we hope to guide healthcare practitioners in considering them through a thorough evaluation of benefits and risks on a case-by-case basis, with relevance both in the U.S. and internationally.
Impact of Vision Defects on Tooth Shade Selection: A Comparative of Spectrophotometry and Shade Guides in a Cross-Sectional Clinical Study
Authors: Alvarado-Lorenzo Mario , Lozano-Garcia Eva , Colino-Gallardo Pedro , Pellicer Castillo Luis Daniel , Díaz-Flores García Victor , Alvarado-Lorenzo Alfonso
Published: January 2025

Introduction: Tooth shade selection is a fundamental factor in the success of dental restorations, and visual impairment may adversely affect this process. The aim of this cross-sectional clinical study was to determine whether visual impairment influences shade selection using two methods: spectrophotometry and shade guides. Materials and Methods: The sample consisted of 2796 maxillary and mandibular teeth, and shade selection was measured subjectively with a shade guide (VITA Classic, VITA Zahnfabrik) and objectively with a spectrophotometer (VITA Easyshade® V, VITA Zahnfabrik, Bad Säckingen, Germany). In all cases, three measurements were taken on each tooth, with a waiting time of 15 min between samples. Shade selection was compared between observers with normal vision, myopia, astigmatism, and hyperopia. Results: The results show that myopic subjects perceived the lower central incisors (2.63, p < 0.05), upper lateral incisors (2.42, p < 0.05), lower lateral incisors (2.34, p < 0.05), and lower canines (2.64, p < 0.05) more clearly. Non-astigmatic subjects perceived the lower second premolar as lighter than astigmatic subjects (−2.01, p < 0.05). Conclusions: It can be concluded that myopes see teeth more clearly, but no differences have been found in astigmatism and hyperopes.
Cognitive and Emotional Resilience in Parents with Children with Autism Spectrum Disorder During COVID-19: The Role of Promoting Variables
Authors: Sarhani-Robles Aziz , Guillot-Valdés María , Robles-Bello María Auxiliadora , Sánchez-Teruel David
Published: January 2025

The pandemic resulting from the coronavirus disease (COVID-19) has entailed social and psychological consequences for the Spanish population, with children with autism spectrum disorder (ASD) being particularly vulnerable due to their genetic characteristics. The present study focuses on the efforts of parents of children with autism spectrum disorder to improve their situation during the pandemic. In particular, the objective is to identify promoting variables (subjective well-being, positive mental health, social support, humour, cognitive reappraisal, and self-esteem) and sociodemographic variables that predict resilience, marking positive coping with this adverse situation. Furthermore, the study conceptually explores the potential role of emotional intelligence in resilience-building processes. We hypothesised that higher scores in these promoting variables would predict greater resilience, with emotional intelligence potentially serving as an underlying framework. The methodology employed in this study is as follows: A cross-sectional predictive study was conducted on a sample of 799 parents using an online questionnaire administered during the social confinement resulting from the SARS-CoV-2 pandemic. The information analysed is based on data provided by the parents. Statistical methods included Student’s t-tests, Pearson’s correlations, and stepwise multivariate regression analysis to identify predictors of resilience. The results of the study are presented below. Significant resilience scores and resilience prediction were identified in participants based on positive mental health, emotion regulation, sense of humour, social support, age, and employment status (self-employed). Relations with emotional intelligence were identified, particularly in the domains of emotion regulation, cognitive reappraisal, and positive mental health. Discussion: The necessity of an intervention that prioritises the empowerment of resilience in the target population is substantiated. Practical implications suggest leveraging emotional intelligence strategies to enhance resilience in this population. This study highlights the importance the aforementioned variables, in addition to potential strategies for enhancing the sociodemographic circumstances of the families.
Preference for On-Demand Fexuprazan Therapy in Gastroesophageal Reflux Disease: A Prospective Cohort Study
Authors: Jung Byung Wook , Park Chan Hyuk , Eun Chang Soo
Published: January 2025

Introduction: Maintenance therapy is crucial in managing and preventing symptom relapse in gastroesophageal reflux disease (GERD), with continuous and on-demand therapy being the common approaches. However, maintenance therapy using potassium-competitive acid blockers (P-CABs), such as fexuprazan, remains incompletely evaluated. Methods: This single-center, single-arm, prospective cohort study enrolled individuals with weekly heartburn or acid regurgitation and confirmed erosive esophagitis. The participants received 40 mg fexuprazan daily for 4 weeks as initial therapy, followed by 4 weeks of maintenance therapy. Patients chose either continuous or on-demand therapy for maintenance, according to their preference. The primary endpoint was the proportion of patients selecting on-demand therapy. The symptom scores were assessed using the GERD questionnaire (GERD-Q) and patient assessment of upper-gastrointestinal-disorders symptoms questionnaire (PAGI-SYM). Results: The 31 included participants showed a significant reduction in symptom scores after initial treatment (baseline vs. 4-week: GERD-Q, 9.0 vs. 6.5, p < 0.001; PAGI-SYM, 29.0 vs. 10.8, p < 0.001). Twenty-one (67.7%) patients chose on-demand therapy after initial treatment. The symptom scores did not differ significantly before and after maintenance therapy (4-week vs. 8-week: GERD-Q, 6.5 vs. 6.0, p = 0.225; PAGI-SYM, 10.8 vs. 9.0, p = 0.354). Although this relation was not significant, patients experiencing larger decreases in symptom scores tended to prefer on-demand therapy. After maintenance therapy, the symptom scores did not differ between continuous and on-demand therapy (GERD-Q, 5.3 vs. 6.3, p = 0.342; PAGI-SYM, 9.4 vs. 8.8, p = 0.611). Conclusions: Fexuprazan was effective as an initial and maintenance therapy in patients with GERD who showed typical symptoms. Approximately 68% of the patients preferred on-demand therapy as a maintenance treatment. Based on the patient’s preference for maintenance therapy, symptom control did not differ between continuous and on-demand therapy.
Stablecoin: A Story of (In)Stabilities and Co-Movements Written Through Wavelet
Authors: Moura de Carvalho Rubens , Inácio Helena Coelho , Marques Rui Pedro
Published: January 2025

Stablecoins are crypto assets designed to maintain stable value by bridging fiat currencies and volatile crypto assets. Our study extends previous research by analyzing the instability and co-movement of major stablecoins (USDT, USDC, DAI, and TUSD) during significant economic events such as the COVID-19 pandemic and the collapses of Iron Finance, Terra-Luna, FTX, and Silicon Valley Bank (SVB). We investigated the temporal volatility and dynamic connections between stablecoins using wavelet techniques. Our results showed that the announcement of USDT’s listing on Coinbase in April 2021 significantly impacted the stability of stablecoins, evidenced by a decline in the power spectrum. This phenomenon has not been explored in the literature. Furthermore, the collapse of SVB was highly relevant to the stablecoin market. We observed high coherence between pairs during the pandemic, the Coinbase listing, and the collapse of SVB. After the collapse of Terra-Luna, USDT, USDC, and DAI became more connected in the medium term, with USDC and DAI extending in the long term despite a negative co-movement between USDT and the others. This study highlights the impact of exchange listings on the volatility of stablecoins, with implications for investors, regulators, and the cryptocurrency community, especially regarding the stability and safe integration of these assets into the financial system.
Assessment of Soil Heavy Metal Pollution and Phytoremediation Potential of Dominant Species in Water-Level-Fluctuation Zone of Large Reservoir
Authors: Li Wenqiao , Zuo Youwei , Li Yunpeng , Ning Denghao , Zhang Huan , Xia Changying , Zhang Xiao , Deng Hongping
Published: January 2025

The water-level-fluctuation zone of a reservoir is an important connection between land and water; due to counter-seasonal rhythmic water level changes, this area is more sensitive and vulnerable, whereas the assessment and management of heavy metal pollution are still elusive. This study selected the water-level-fluctuation zone in the hinterland of the Three Gorges Reservoir. Through a quadrats investigation, the soil pollution status of seven heavy metals and the composition of herbaceous plants were identified, and the potential of the dominant species for phytoremediation was assessed. The results showed that the overall pollution was at the alert level (PN = 0.97) and a low ecological risk level (RI = 123.12). And the study area was primarily contaminated by a mixed source of traffic, agriculture, and industry. A total of 17 dominant species were calculated using importance values. Most dominant species had better phytoremediation potential for Cd and Cu. None of the 17 dominant species were hyperaccumulators, but they could still play an important role in phytoremediation due to their high adaptability to specific environments. The results have guiding significance for the management and remediation of soil heavy metal pollution in global reservoir areas, further promoting global ecological security and sustainable development.
Measuring Accessibility of Green Spaces for the Health and Wellbeing of Inhabitants of the Milan Metropolitan Area
Authors: Pastore Maria Chiara , Parenti Claudia Ida Maria , Patetta Corinna
Published: January 2025

Several studies have recognised the importance of urban green spaces (UGSs) in modern cities. However, the distribution and accessibility of green spaces represent a challenge for the sustainable development of territories in terms of fair distribution, reducing inequalities, and mitigating the effects of climate change, such as urban heat islands and runoff. This research identifies every UGS capable of contributing to people’s physical and mental wellbeing in the Milan metropolitan area (MMA), one of the most densely populated areas in Europe. The method allows for the various UGSs to be identified and classified using a comparative approach that considers both formal and informal green spaces through a qualitative territorial analysis and the support of open access databases (satellite and thematic maps). Based on this classification, this contribution establishes an ‘accessibility map’ of the metropolitan area that determines the actual pedestrian accessibility of a UGS within 300 m. The results show that only 37% of the residential surface of the metropolitan area offers access to a UGS within a five-minute walk. This research aims to identify the most fragile segment of the MMA, which is pivotal for the tree-planting activities sponsored by the Forestami project.
Differences Between Amblyopic and Fellow Eyes in Optical Coherence Tomography: A Cohort from Jordanian Population
Authors: Alqudah Noor M. , Mohidat Hasan , Aleshawi Abdelwahab , Al-Dolat Wedad , Alshami Ali
Published: January 2025

Background and Objectives: Amblyopia is a condition where children undergo unilateral or bilateral vision loss due to a variety of disorders that impact the visual pathway. The assessment of retinal nerve fiber layer (RNFL) thickness in amblyopia has made optical coherence tomography (OCT) a useful technique for studying the pathophysiology of this condition. This study was conducted to assess OCT results for various forms of amblyopia, including macular thickness and peripapillary RNFL thickness. It is the first of its kind in Jordan. Materials and Methods: Sixty-one individuals, aged 14 to 67, who had been diagnosed with unilateral amblyopia participated in a prospective study. Both eyes underwent spectral-domain OCT imaging, and clinical and demographic information was gathered. Relationships between different types of amblyopia and OCT measurements were investigated. Results: Compared to the contralateral fellow eyes, the amblyopic eyes showed thicker fovea, and there were differences in foveal and macular thickness observed between the sexes. Individuals with anisometropic amblyopia exhibited a greater central macular thickness. While nasal optic nerve thickness was adversely correlated with age, inferior optic nerve thickness was considerably lower in amblyopia. Conclusions: This research indicates unique OCT traits in amblyopic eyes, which may have a role in amblyopia diagnosis and treatment. The prevention of long-term visual impairment depends heavily on early detection and care. More studies using larger cohorts and longitudinal designs are necessary to improve our knowledge of the pathogenesis of amblyopia and to provide the best possible clinical management approaches.
Comparison of Secondary Metabolite Extraction Methods in Hamelia patens Jacq. and Their Inhibitory Effect on Fusarium oxysporum f. sp. radicis-lycopersici
Authors: Valle Ortiz Daniel Jafet , Aguila Muñoz Dolores Guadalupe , Cruz López María del Carmen , Cortés Espinosa Diana Verónica , Rosales Castro Martha , Jiménez Montejo Fabiola Eloísa
Published: January 2025

Background: Hamelia patens Jacq. (HP) is widely recognized in traditional medicine for its antimicrobial properties, which are attributed to secondary metabolites such as phenolic compounds, alkaloids, and terpenes. Fusarium oxysporum f. sp. radicis-lycopersici (Fo), a phytopathogenic fungus affecting economically important crops, is managed with fungicides like benzimidazoles and azoles. Excessive use of these compounds has led to resistance and environmental contamination, highlighting the need for sustainable alternatives. This study aimed to optimize the extraction of secondary metabolites from HP leaves and flowers, evaluate their antifungal activity, and assess the impact of extraction methods and plant parts on chemical composition and efficacy. Methods: Three extraction methods were employed: consecutive maceration (CM) using solvents of ascending polarity; total maceration (TM), which is a single-step methanol-based method; and ultrasound-assisted maceration (UAM) employing ultrasonic waves with methanol. Extracts were characterized by quantifying total phenols (TP), condensed tannins (TC), flavonoids (Fl), alkaloids (TA), sterols (TS), and saponins (S) using colorimetric assays and UPLC-MS. Multivariate analyses, including PCA, PLS-DA, OPLS-DA, and Pearson correlation, evaluated the relationships between the chemical profiles and antifungal activity. Results: Leaf extracts exhibited higher flavonoid and tannin contents than flower extracts. CMML showed the highest antifungal activity (IC50 3.7% w/v), which was associated with elevated levels of these compounds. Significant correlations linked antifungal activity with rutin (HP21) and kaempferol-3-O-β-rutinoside (HP29). Conclusions: Methanolic extracts of HP exhibited significant antifungal activity against Fo. These findings highlight the importance of optimizing extraction methods and selecting specific plant parts to enhance bioactive compound efficacy, offering a sustainable approach to pathogen management.
Analysis of the Genomes and Adaptive Traits of Skermanella cutis sp. nov., a Human Skin Isolate, and the Type Strains Skermanella rosea and Skermanella mucosa
Authors: Choi Yujin , Ganzorig Munkhtsatsral , Lee Kyoung
Published: January 2025

The genus Skermanella comprises important soil bacteria that are often associated with the crop rhizospheres, but its physiological traits remain poorly understood. This study characterizes Skermanella sp. TT6T, isolated from human skin, with a focus on its metabolic and environmental adaptations. Genome sequencing and phylogenomic analyses revealed that the strain TT6T is most closely related to S. rosea M1T, with average nucleotide identity and digital DNA–DNA hybridization values of 94.14% (±0.5%) and 64.7%, respectively. Comparative genomic analysis showed that the strains TT6T, S. rosea M1T and S. mucosa 8-14-6T share the Calvin cycle, and possess photosynthetic genes associated with the purple bacteria-type photosystem II. The strains TT6T and S. rosea M1T exhibited growth in a nitrogen-free medium under microaerobic conditions, which were generated in test tubes containing 0.1% soft agar. Under these conditions, with nitrate as a nitrogen source, S. rosea M1T formed gases, indicating denitrification. Strain TT6T also contains gene clusters involved in trehalose and carotenoid biosynthesis, along with salt-dependent colony morphology changes, highlighting its adaptive versatility. Genomic analyses further identified pathways related to hydrogenase and sulfur oxidation. Phenotypic and chemotaxonomic traits of strain TT6T were also compared with closely related type strains, confirming its genotypic and phenotypic distinctiveness. The new species, Skermanella cutis sp. nov., is proposed, with TT6T (=KCTC 82306T = JCM 34945T) as the type strain. This study underscores the agricultural and ecological significance of the genus Skermanella.