bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and Safety of Lenvatinib in Hepatocellular Carcinoma Patients with Liver Transplantation: A Case-Control Study
Authors: Chen Yen-Yang , Chen Chao-Long , Lin Chih-Che , Wang Chih-Chi , Liu Yueh-Wei , Li Wei-Feng , Chen Yen-Hao
Published: September 2021

Tumor recurrence is the most common cause of death in hepatocellular carcinoma (HCC) patients who received liver transplantation (LT). Recently, lenvatinib was approved for the systemic treatment of unresectable HCC patients; however, the role of lenvatinib in HCC patients after LT remains unclear. There were 56 patients with recurrent HCC after LT from 2008 to 2018 in our institute, and 10 patients who received lenvatinib were identified. Additionally, to understand the difference in the clinical impact of lenvatinib in the LT and non-LT settings, 25 HCC patients without LT who underwent lenvatinib treatment were identified from our HCC database and regarded as the control group. In the LT group, partial response was 20% and stable disease was 50%, resulting in a disease control rate of 70%; the median progression-free survival (PFS), time to treatment failure (TTF) and overall survival (OS) were 3.7, 3.6 and 16.4 months, respectively. Adverse events (AEs) were predominantly grade 1–2 in severity, and the majority of patients tolerated the side effects. There was no significant difference in PFS/OS, and we observed a similar pattern of AEs between these two groups. Our study confirms the comparable efficacy and safety of lenvatinib in HCC patients with LT and non-LT in clinical practice.
Enhanced Visible Light Photocatalytic Degradation of Methylene Blue by CdS-ZnS-BiPO4 Nanocomposites Prepared by a Solvent-Assisted Heating Method
Authors: Tsai Hsin-Chan , Peng Yu-Hui , Wen Po-Yu , Wu Tsunghsueh , Lin Yang-Wei
Published: September 2021

In this study, a ternary CdS-ZnS-BiPO4 nanocomposite, synthesized by a solvent-assisted heating method, demonstrated the highest visible light-induced photocatalysis towards the degradation of methylene blue (MB) when comparing with BiPO4, CdS-BiPO4, and ZnS-BiPO4. Transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and UV-Vis diffuse reflectance spectroscopy (UV-vis DRS) were used to characterize the prepared nanocomposites. From UV-DRS results, the energy band gap of the prepared BiPO4 structures was 4.51 eV. When CdS nanoparticles were deposited on BiPO4 surface by a solvent-assisted heating method, the prepared nanocomposites exhibited visible light-responsive photocatalytic degradation toward MB (20 ppm). At a molar ratio of Cd to Zn as 1:7, the prepared CdS-ZnS-BiPO4 nanocomposites exhibited the best photocatalytic activity in degrading 95% of MB dyes, out-performing pure BiPO4, CdS-BiPO4, and ZnS-BiPO4 due to its enhanced charge separation efficiency and the lowered carrier recombination from the efficient p-n junction of unprecedented ternary composites. The investigations on mechanism conclude that the major reactive species responsible for MB degradation are holes and oxygen radicals. For practicality, the degradation efficiency for different dyestuff (Fast Green FCF, Rhodamine 6G, Acid Blue 1, methyl orange, and methyl red) degradation in the different water matrix samples (pond water, seawater, and lake water) by the prepared CdS-ZnS-BiPO4 nanocomposites was evaluated.
The Pathogenic and Therapeutic Implications of Ceramide Abnormalities in Atopic Dermatitis
Authors: Fujii Masanori
Published: September 2021

Ceramides play an essential role in forming a permeability barrier in the skin. Atopic dermatitis (AD) is a common chronic skin disease associated with skin barrier dysfunction and immunological abnormalities. In patients with AD, the amount and composition of ceramides in the stratum corneum are altered. This suggests that ceramide abnormalities are involved in the pathogenesis of AD. The mechanism underlying lipid abnormalities in AD has not yet been fully elucidated, but the involvement of Th2 and Th1 cytokines is implicated. Ceramide-dominant emollients have beneficial effects on skin barrier function; thus, they have been approved as an adjunctive barrier repair agent for AD. This review summarizes the current understanding of the mechanisms of ceramide abnormalities in AD. Furthermore, the potential therapeutic approaches for correcting ceramide abnormalities in AD are discussed.
Dynamic Regulation of Cysteine Oxidation and Phosphorylation in Myocardial Ischemia–Reperfusion Injury
Authors: Casin Kevin M. , Calvert John W.
Published: September 2021

Myocardial ischemia–reperfusion (I/R) injury significantly alters heart function following infarct and increases the risk of heart failure. Many studies have sought to preserve irreplaceable myocardium, termed cardioprotection, but few, if any, treatments have yielded a substantial reduction in clinical I/R injury. More research is needed to fully understand the molecular pathways that govern cardioprotection. Redox mechanisms, specifically cysteine oxidations, are acute and key regulators of molecular signaling cascades mediated by kinases. Here, we review the role of reactive oxygen species in modifying cysteine residues and how these modifications affect kinase function to impact cardioprotection. This exciting area of research may provide novel insight into mechanisms and likely lead to new treatments for I/R injury.
Upregulation of Low-Density Lipoprotein Receptor of the Steroidogenesis Pathway in the Cumulus Cells Is Associated with the Maturation of Oocytes and Achievement of Pregnancy
Authors: Kim Myung Joo , Kim Young Sang , Kim Yu Jin , Lee Hye Ran , Choi Kyoung Hee , Park Eun A , Kang Ki Ye , Yoon Tae Ki , Hwang Sohyun , Ko Jung Jae , Kim You Shin , Lee Jae Ho
Published: September 2021

The maturation of the oocyte is influenced by cumulus cells (CCs) and associated with pregnancy rate, whereas the influencing factors have not been completely elucidated in the CCs. In this study, we identified new regulators of CCs for high-quality oocytes and successful pregnancies during assisted reproductive techniques. CCs were collected from cumulus–oocyte complexes (COCs) in young (≤33 years old) and old (≥40 years old) women undergoing intracytoplasmic sperm injection (ICSI) procedures. We screened for factors differentially expressed between young vs. old CCs and pregnancy vs. non-pregnancy using whole mRNA-seq-next-generation sequencing (NGS). We characterized the transcriptome of the CCs to identify factors critical for achieving pregnancy in IVF cycles. Women in the young and old pregnancy groups exhibited the up- and downregulation of multiple genes compared with the non-pregnancy groups, revealing the differential regulation of several specific genes involved in ovarian steroidogenesis in CCs. It was shown that the low-density lipoprotein (LDL) receptor to the steroidogenesis pathway was upregulated in CCs with higher maturity rates of oocytes in the pregnancy group. In conclusion, a higher pregnancy rate is related to the signaling pathway of steroidogenesis by the LDL receptor in infertile women undergoing IVF procedures.
26S Proteasome Non-ATPase Regulatory Subunits 1 (PSMD1) and 3 (PSMD3) as Putative Targets for Cancer Prognosis and Therapy
Authors: Rubio Andres J. , Bencomo-Alvarez Alfonso E. , Young James E. , Velazquez Vanessa V. , Lara Joshua J. , Gonzalez Mayra A. , Eiring Anna M.
Published: September 2021

Ever since the ubiquitin proteasome system was characterized, efforts have been made to manipulate its function to abrogate the progression of cancer. As a result, the anti-cancer drugs bortezomib, carfilzomib, and ixazomib targeting the 26S proteasome were developed to treat multiple myeloma, mantle cell lymphoma, and diffuse large B-cell lymphoma, among others. Despite success, adverse side effects and drug resistance are prominent, raising the need for alternative therapeutic options. We recently demonstrated that knockdown of the 19S regulatory components, 26S proteasome non-ATPase subunits 1 (PSMD1) and 3 (PSMD3), resulted in increased apoptosis of chronic myeloid leukemia (CML) cells, but had no effect on normal controls, suggesting they may be good targets for therapy. Therefore, we hypothesized that PSMD1 and PSMD3 are potential targets for anti-cancer therapeutics and that their relevance stretches beyond CML to other types of cancers. In the present study, we analyzed PSMD1 and PSMD3 mRNA and protein expression in cancerous tissue versus normal controls using data from The Cancer Genome Atlas (TCGA) and the Clinical Proteomic Tumor Analysis Consortium (CPTAC), comparing expression with overall survival. Altogether, our data suggest that PSMD1 and PSMD3 may be novel putative targets for cancer prognosis and therapy that are worthy of future investigation.
Synergistic Improvement in Coating with UV Aging Resistance and Anti-Corrosion via La-Doped CeO2 Powders
Authors: Tian Ang , Ma Tengda , Shi Xiaoguo , Wang Dixiang , Wu Wenyuan , Liu Chuangwei , Pei Wenli
Published: September 2021

Benefitting from a suitable band gap, ceria is an excellent material for UV shielding. By solid solution doping and specific micromorphology, its band gap can be effectively controlled. In this paper, ceria doped with lanthanum via oxalate precipitation is combined with a high-temperature roasting process. The properties of the prepared samples are characterized by UV–Vis diffuse reflectance spectroscopy (DRS), Raman, XRD, FESEM and XPS. The absorption threshold of materials is clearly red-shifted in the ultraviolet band, which originates from the electron-phonon generation. To further reveal the mechanism, the density function theory calculation (DFT) is implemented to study the influence of lanthanum concentrations on ceria’s band gap. It is demonstrated that the band gap can even be narrowed to 2.97 eV by optimizing the sintering temperature and lanthanum-doped concentration. To investigate its improved anti-aging properties under ultraviolet rays, different amounts of 5% lanthanum-doped ceria is mixed with an Al-based coating and then coated on the Q235 steel. Combined with an ultraviolet light irradiation experiment and electrochemical test technology, the corrosion resistance of the modified coatings is evaluated. The coating with 20% La-doped ceria provides the best corrosion resistance performance.
Spectroscopic Investigations of 316L Stainless Steel under Simulated Inflammatory Conditions for Implant Applications: The Effect of Tryptophan as Corrosion Inhibitor/Hydrophobicity Marker
Authors: Święch Dominika , Palumbo Gaetano , Piergies Natalia , Pięta Ewa , Szkudlarek Aleksandra , Paluszkiewicz Czesława
Published: September 2021

In this paper, the conformational changes of tryptophan (Trp) on the corroded 316 L stainless steel (SS) surface obtained under controlled simulated inflammatory conditions have been studied by Raman (RS) and Fourier-transform infrared (FT-IR) spectroscopy methods. The corrosion behavior and protective efficiency of the investigated samples were performed using the potentiodynamic polarization (PDP) technique in phosphate-buffered saline (PBS) solution acidified to pH 3.0 at 37 °C in the presence and absence of 10−2 M Trp, with different immersion times (2 h and 24 h). The amino acid is adsorbed onto the corroded SS surface mainly through the lone electron pair of the nitrogen atom of the indole ring, which adopts a more/less tilted orientation, and the protonated amine group. The visible differences in the intensity of the Fermi doublet upon adsorption of Trp onto the corroded SS surface, which is a sensitive marker of the local environment, suggested that a stronger hydrophobic environment is observed. This may result in an improvement of the corrosion resistance, after 2 h than 24 h of exposure time. The electrochemical results confirm this statement—the inhibition efficiency of Trp, acting as a mixed-type inhibitor, is made drastically higher after a short period of immersion.
Transport Mechanism of Enhanced Performance in an Amorphous/Monoclinic Mixed-Phase Ga2O3 Solar-Blind Deep Ultraviolet Photodetector
Authors: Liu Haowen , Li Honglin , Zhou Shuren , Zhang Hong , Fan Shiqiang , Cui Yuting , Kong Chunyang , Ye Lijuan , Xiong Yuanqiang , Li Wanjun
Published: September 2021

Recently, as an emerging material, ultrawide bandgap Ga2O3 has been investigated extensively in solar-blind deep-ultraviolet (DUV) photodetectors (PDs). High sensitivity and signal-to-noise ratio of PDs are essential for the detection of solar-blind DUV signals; however, such factors are often not mutually compatible. In the present study, an amorphous/monoclinic homogeneous mixed-phase structure was demonstrated to be significantly beneficial in enhancing the comprehensive performance of Ga2O3 solar-blind DUV PDs, especially with respect to sensitivity and the signal-to-noise ratio. Further experimental and theoretical findings provide insights on the transport mechanism of enhanced performance in the mixed-phase Ga2O3 solar-blind DUV PD. For effectively separating the photogenerated carriers, a type-II band alignment between amorphous and crystalline Ga2O3 can be exploited. Furthermore, the change of the barrier height of the mixed-phase interface also has a significant impact on the transport properties of the mixed-phase Ga2O3 PD. Additionally, the potential applications of mixed-phase Ga2O3 PD in high-voltage corona discharge were explored, and clear and stable corona discharge signals were obtained. The results of the present study may promote understanding of DUV photoelectronic devices with various mixed-phase Ga2O3 materials and provide an efficient approach for promoting comprehensive performance in future solar-blind detection applications.
Feasibility of TSPO-Specific Positron Emission Tomography Radiotracer for Evaluating Paracetamol-Induced Liver Injury
Authors: Kim Daehee , Moon Byung Seok , Park Sun Mi , Lee Sang Ju , Kang Seo Young , Park Sanghui , Oh Seung Jun , Kim Bom Sahn , Yoon Hai-Jeon
Published: September 2021

Macrophages are activated during the early phase of paracetamol-induced liver injury (PLI). [18F]GE180 is a radiolabeled ligand that recognizes the macrophage translocator protein (TSPO). In this study, we evaluated the feasibility of a TSPO-specific radiotracer in a rat model of PLI. A rat model of liver injury was induced by intraperitoneal administration of paracetamol. [18F]GE180 positron emission tomography (PET) images were obtained after 24 h. The maximal and mean standardized uptake values (SUVmax and SUVav) of the liver and serum biomarker levels were examined. The TSPO expression level was examined using real-time polymerase chain reaction and Western blot analysis. [18F]GE180 hepatic uptake in the PLI group was significantly higher than that in the control group (SUVmax p = 0.001; SUVav p = 0.005). Both mRNA and protein TSPO expression levels were higher in the PLI group. The mRNA expression level of TSPO was significantly correlated with [18F]GE180 hepatic uptake in both groups (SUVmax p = 0.019; SUVav p = 0.007). [18F]GE180 hepatic uptake in the PLI group showed a significant positive correlation with ALT24 and ALT48 (ALT24 p = 0.016; ALT48p = 0.002). [18F]GE180 enabled visualization of PLI through TSPO overexpression. Our results support the potential utility of hepatic uptake by TSPO-PET as a non-invasive imaging biomarker for the early phase of PLI.
Automated Drone Detection Using YOLOv4
Authors: Singha Subroto , Aydin Burchan
Published: September 2021

Drones are increasing in popularity and are reaching the public faster than ever before. Consequently, the chances of a drone being misused are multiplying. Automated drone detection is necessary to prevent unauthorized and unwanted drone interventions. In this research, we designed an automated drone detection system using YOLOv4. The model was trained using drone and bird datasets. We then evaluated the trained YOLOv4 model on the testing dataset, using mean average precision (mAP), frames per second (FPS), precision, recall, and F1-score as evaluation parameters. We next collected our own two types of drone videos, performed drone detections, and calculated the FPS to identify the speed of detection at three altitudes. Our methodology showed better performance than what has been found in previous similar studies, achieving a mAP of 74.36%, precision of 0.95, recall of 0.68, and F1-score of 0.79. For video detection, we achieved an FPS of 20.5 on the DJI Phantom III and an FPS of 19.0 on the DJI Mavic Pro.
Role of Natural Capital Economics for Sustainable Management of Earth Resources
Authors: Meraj Gowhar , Kanga Shruti , Kranjčić Nikola , Đurin Bojan , Singh Suraj Kumar
Published: September 2021

Natural capital is the wealth of nations that determine their economic status. Worldwide, vulnerable people depend on natural capital for employment, salaries, wealth, and livelihoods and, in turn, this determines the developmental index of the nation to which they belong. In this short review, we have tried to sum up the ideas and discussions over natural capital’s role in ascribing economic status to countries as well as the need for natural resource management and sustainability. This paper aimed to discuss how humanity’s prosperity is intertwined with the services that ecosystems provide, and how poor natural resource management (NRM) has adversely affected human well-being. Our preselected criteria for the review paper led us to evaluate 96 peer-reviewed publications from the SCOPUS database, which is likely the most comprehensive archive of peer-reviewed scientific literature as well as WoS, PUBMED, and Google Scholar databases. Our review revealed that the availability of ecological services is crucial for clean water and air, food and fodder, and agricultural development. We further discussed important concepts regarding sustainability, natural capital and economics, and determinants of human well-being vis-à-vis the intergenerational security of natural wealth. To ensure current and future human well-being, we conclude that an in-depth understanding of the services that ecosystems provide is necessary for the holistic management of the Earth system.
Macroeconomic Efficiency of Photovoltaic Energy Production in Polish Farms
Authors: Bukowski Marcin , Majewski Janusz , Sobolewska Agnieszka
Published: September 2021

The public’s awareness of threats to the natural environment, as well as the hazard to human lives and health posed by the use of fossil fuels to generate energy has resulted in the growing interest in renewable energy sources, thus promoting attempts to reduce the dependency on conventional energy sources. Among the former, solar energy is one of the most promising. The aim of this study is to assess the macroeconomic efficiency of investments in photovoltaic installations to meet the demand for electricity of farms and agricultural production. Calculations were prepared for 48 variants comprising three farm types (dairy farms, field cropping farms, and mixed production farms), as well as 16 locations throughout Poland. The obtained results indicate high efficiency of electricity production using photovoltaic installations to cover the needs of farms in Poland. In macroeconomic accounting, NPV ranges from EUR 8200 to almost EUR 23,000, with the payback period depending on the farm type ranging from 4.3 up to 6 years, while the internal rate of return amounts to 21–32%. Increasing the scope of investments in photovoltaics (PV) to cover the electricity demand not only of the household, but also of the agricultural production leads to improved economic efficiency of energy production both in the macro- and microeconomic terms.
Energy Management System for Hybrid PV/Wind/Battery/Fuel Cell in Microgrid-Based Hydrogen and Economical Hybrid Battery/Super Capacitor Energy Storage
Authors: Sahri Younes , Belkhier Youcef , Tamalouzt Salah , Ullah Nasim , Shaw Rabindra Nath , Chowdhury Md. Shahariar , Techato Kuaanan
Published: September 2021

The present work addresses the modelling, control, and simulation of a microgrid integrated wind power system with Doubly Fed Induction Generator (DFIG) using a hybrid energy storage system. In order to improve the quality of the waveforms (voltages and currents) supplied to the grid, instead of a two level-inverter, the rotor of the DFIG is supplied using a three-level inverter. A new adaptive algorithm based on combined Direct Reactive Power Control (DRPC) and fuzzy logic controls techniques is applied to the proposed topology. In this work, two topologies are proposed. In the first one, the active power injected into the grid is smoothened by using an economical hybrid battery and supercapacitor energy storage system. However, in the second one, the excess wind energy is used to produce and store the hydrogen, and then a solid oxide fuel cell system (SOFC) is utilized to regenerate electricity by using the stored hydrogen when there is not enough wind energy. To avoid overcharging, deep discharging of batteries, to mitigate fluctuations due to wind speed variations, and to fulfil the requirement of the load profile, a power management algorithm is implemented. This algorithm ensures smooth output power in the first topology and service continuity in the second. The modelling and simulation results are presented and analysed using Matlab/Simulink.
Environmental Changes Produced by Household Consumption
Authors: Martínez Miguel , Cámara Ángeles
Published: September 2021

This paper analyzes the impact of the fall in household consumption after an economic crisis in Spain on greenhouse gas emissions. To this end, household consumption is differentiated by the age of the main provider by using a conversion matrix that relates consumption groups to activity sectors. A multisectoral model was used to quantify and compare the environmental impact caused by the consumption of each age group, indicating that the older the age of the main household provider, the smaller the reduction in GHG emissions associated with their consumption. The results facilitate an analysis of how the greenhouse gas emissions of the different sectors of the Spanish economy, associated with the population under study, varied before and after the 2008 crisis, and confirm that the sectors with the greatest reduction in emissions were, in this order, extractive industries, construction, manufacturing industry, wholesale and retail trade and transport and storage. This is relevant for decision making in the field of environmental policies in crises, akin to the one the world is currently experiencing.
Generation Expansion Planning with Energy Storage Systems Considering Renewable Energy Generation Profiles and Full-Year Hourly Power Balance Constraints
Authors: Diewvilai Radhanon , Audomvongseree Kulyos
Published: September 2021

This paper proposes a methodology to develop generation expansion plans considering energy storage systems (ESSs), individual generation unit characteristics, and full-year hourly power balance constraints. Generation expansion planning (GEP) is a complex optimization problem. To get a realistic plan with the lowest cost, acceptable system reliability, and satisfactory CO2 emissions for the coming decades, a complex multi-period mixed integer linear programming (MILP) model needs to be formulated and solved with individual unit characteristics along with hourly power balance constraints. This problem requires huge computational effort since there are thousands of possible scenarios with millions of variables in a single calculation. However, in this paper, instead of finding the globally optimal solutions of such MILPs directly, a simplification process is proposed, breaking it down into multiple LP subproblems, which are easier to solve. In each subproblem, constraints relating to renewable energy generation profiles, charge-discharge patterns of ESSs, and system reliability can be included. The proposed process is tested against Thailand’s power development plan. The obtained solution is almost identical to that of the actual plan, but with less computational effort. The impacts of uncertainties as well as ESSs on GEP, e.g., system reliability, electricity cost, and CO2 emission, are also discussed.
Parameter Extraction of Photovoltaic Cells and Modules Using Grey Wolf Optimizer with Dimension Learning-Based Hunting Search Strategy
Authors: Yesilbudak Mehmet
Published: September 2021

With the increase in the share of solar energy in the sustainable development, accurate parameter identification plays a significant role in designing optimal solar photovoltaic systems. For this purpose, this paper extensively implements and evaluates the grey wolf optimizer with a dimension learning-based hunting search strategy, an improved version of GWO named I-GWO, in the parameter extraction of photovoltaic cells and modules. According to the experimental results, the double-diode model leads to better fitness than the other diode models in representing the physical behaviors of both photovoltaic cells and photovoltaic modules. For further performance validation, firstly, the internal parameters extracted by the I-GWO algorithm and the corresponding output current data are compared with a number of widely-used parameter extraction methods in the literature. Then, the best goodness-of-fit results achieved by the I-GWO algorithm are evaluated considering many state-of-the-art metaheuristic algorithms in the literature. The accuracy measures including root mean squared error and sum of individual absolute errors show that I-GWO is fairly promising to be the efficient and valuable parameter extraction method for both photovoltaic cells and photovoltaic modules.
Thermographic Measurement of the Temperature of Reactive Power Compensation Capacitors
Authors: Dziarski Krzysztof , Hulewicz Arkadiusz , Dombek Grzegorz
Published: September 2021

An excessive increase in reactive power consumption is unfavorable from the point of view of a power system. For this reason, devices compensating reactive power consumption are used. The capacitor is one such device. Capacitors must be tested regularly during their exploitation. One of the activities that should be performed is testing the degree of heating of the cells of a capacitor bank. Thermography can be used to perform such tests. This non-contact method has its limitations. Due to the angular emissivity and the change in the distance between the lens and the object under observation, the temperature measured with a thermographic camera may differ from the actual temperature. This phenomenon is visible on cylindrical capacitor cases. Consequently, depending on the location of the observation point on the capacitor case, the result of the thermographic temperature measurement may be different. To investigate this phenomenon, experimental work has been undertaken.
Advances in Wine Fermentation
Authors: Maicas Sergi
Published: September 2021

Fermentation is a well-known natural process that has been used by humanity for thousands of years, with the fundamental purpose of making alcoholic beverages such as wine, and also other non-alcoholic products. From a strictly biochemical point of view, fermentation is a process of central metabolism in which an organism converts a carbohydrate, such as starch or sugar, into an alcohol or an acid. The fermentation process turns grape juice (must) into wine. This is a complex chemical reaction whereby the yeast interacts with the sugars (glucose and fructose) in the must to create ethanol and carbon dioxide. Fermentation processes to produce wines are traditionally carried out with Saccharomyces cerevisiae strains, the most common and commercially available yeast, and some lactic acid bacteria. They are well-known for their fermentative behavior and technological characteristics, which allow obtaining products of uniform and standard quality. However, fermentation is influenced by other factors as well. The initial sugar content of the must and the fermentation temperature are also crucial to preserve volatile aromatics in the wine and retain fruity characters. Finally, once fermentation is completed, and most of the yeast dies, wine evolution continues until the production of the final product.
Wild-Type KRAS Allele Effects on Druggable Targets in KRAS Mutant Lung Adenocarcinomas
Authors: Baldelli Elisa , El Gazzah Emna , Moran John Conor , Hodge Kimberley A. , Manojlovic Zarko , Bassiouni Rania , Carpten John D. , Ludovini Vienna , Baglivo Sara , Crinò Lucio , Bianconi Fortunato , Dong Ting , Loffredo Jeremy , Petricoin Emanuel F. , Pierobon Mariaelena
Published: September 2021

KRAS mutations are one of the most common oncogenic drivers in non-small cell lung cancer (NSCLC) and in lung adenocarcinomas in particular. Development of therapeutics targeting KRAS has been incredibly challenging, prompting indirect inhibition of downstream targets such as MEK and ERK. Such inhibitors, unfortunately, come with limited clinical efficacy, and therefore the demand for developing novel therapeutic strategies remains an urgent need for these patients. Exploring the influence of wild-type (WT) KRAS on druggable targets can uncover new vulnerabilities for the treatment of KRAS mutant lung adenocarcinomas. Using commercially available KRAS mutant lung adenocarcinoma cell lines, we explored the influence of WT KRAS on signaling networks and druggable targets. Expression and/or activation of 183 signaling proteins, most of which are targets of FDA-approved drugs, were captured by reverse-phase protein microarray (RPPA). Selected findings were validated on a cohort of 23 surgical biospecimens using the RPPA. Kinase-driven signatures associated with the presence of the KRAS WT allele were detected along the MAPK and AKT/mTOR signaling pathway and alterations of cell cycle regulators. FoxM1 emerged as a potential vulnerability of tumors retaining the KRAS WT allele both in cell lines and in the clinical samples. Our findings suggest that loss of WT KRAS impacts on signaling events and druggable targets in KRAS mutant lung adenocarcinomas.
Glaucoma Syndromes: Insights into Glaucoma Genetics and Pathogenesis from Monogenic Syndromic Disorders
Authors: Balikov Daniel A. , Jacobson Adam , Prasov Lev
Published: September 2021

Monogenic syndromic disorders frequently feature ocular manifestations, one of which is glaucoma. In many cases, glaucoma in children may go undetected, especially in those that have other severe systemic conditions that affect other parts of the eye and the body. Similarly, glaucoma may be the first presenting sign of a systemic syndrome. Awareness of syndromes associated with glaucoma is thus critical both for medical geneticists and ophthalmologists. In this review, we highlight six categories of disorders that feature glaucoma and other ocular or systemic manifestations: anterior segment dysgenesis syndromes, aniridia, metabolic disorders, collagen/vascular disorders, immunogenetic disorders, and nanophthalmos. The genetics, ocular and systemic features, and current and future treatment strategies are discussed. Findings from rare diseases also uncover important genes and pathways that may be involved in more common forms of glaucoma, and potential novel therapeutic strategies to target these pathways.
The Role of De Novo Variants in Patients with Congenital Diaphragmatic Hernia
Authors: Bendixen Charlotte , Reutter Heiko
Published: September 2021

The genetic etiology of congenital diaphragmatic hernia (CDH), a common and severe birth defect, is still incompletely understood. Chromosomal aneuploidies, copy number variations (CNVs), and variants in a large panel of CDH-associated genes, both de novo and inherited, have been described. Due to impaired reproductive fitness, especially of syndromic CDH patients, and still significant mortality rates, the contribution of de novo variants to the genetic background of CDH is assumed to be high. This assumption is supported by the relatively low recurrence rate among siblings. Advantages in high-throughput genome-wide genotyping and sequencing methods have recently facilitated the detection of de novo variants in CDH. This review gives an overview of the known de novo disease-causing variants in CDH patients.
Long-Term Embrittlement of Ancient Copper and Silver Alloys
Authors: Oudbashi Omid , Wanhill Russell
Published: September 2021

The manifestations of ancient metals’ embrittlement, cracking and fracture, are challenging problems for restorers and conservators, yet the scientific understanding of these problems is limited. In particular, the study and interpretation of fracture surfaces, fractography, is a minor or non-existent consideration for most archaeometallurgical investigations. This paper presents a survey of fractographic analyses, in combination with the more widely used disciplines of microstructural studies, metallography, and chemical analyses for some Old-World copper alloy (bronzes) and high-silver alloy artifacts that have undergone long-term corrosion and embrittlement damage. We show that fractography, as an adjunct to metallography, can improve the interpretation of these types of damage and assist in selecting the best methods for restoration and conservation of the objects made from these alloys.
Multispectral Imaging and p-XRF for the Non-Invasive Characterization of the Anonymous Devotional Painting ‘Maria Santissima delle Grazie’ from Mirabella Imbáccari (Sicily, Italy)
Authors: Privitera Antonella , Alberghina Maria Francesca , Privitera Elèna , Schiavone Salvatore
Published: September 2021

This work presents the results of the in situ, non-invasive diagnostic investigations performed on the canvas oil painting depicting Madonna and Child, venerated as ‘Maria Santissima delle Grazie’ by the local religious community. The work of art (72 cm × 175 cm) is located on the high altar of the main Church in Mirabella Imbáccari, near Catania (Sicily, Italy). The painter is anonymous, and the supposed dating is the late eighteenth century. Although the painting has never been studied before, it has been attributed to a Sicilian workshop in the literature, raising the doubts of the art historian who conducted this study and who hypothesized a Neapolitan manufacture. Furthermore, due to the good conservation state detected by a macroscopic examination, doubts also arose about dating. To shed light on these aspects, a technical-scientific examination proved necessary. Multispectral imaging techniques (IR Reflectography, UV-induced visible Fluorescence, X-ray) are carried out for the study of the execution technique, the identification of underlying remakes, sketch drawing and the evaluation of the conservation conditions. XRF spectrometry analysis is performed for the identification of the chemical elements constituting the pigments (inorganic chromophores). The diagnostic results allowed this research to confirm the dating suggested by the historical-stylistic knowledge and to highlight new technical peculiarities supporting the attribution to a Neapolitan workshop.
Relationship between Parental Involvement and Mathematics Achievement of Chinese Early Adolescents: Multiple Mediating Roles of Mental Health and Mathematics Self-Efficacy
Authors: Huang Feifei , Huang Zhaofeng , Li Zhe , Zhang Minqiang
Published: September 2021

This study conceptualized the multidimensional construct of parental involvement, including cognitive involvement, behavioral involvement, and personal involvement, and examined the mediating effects of student’s mental health and mathematics self-efficacy. Questionnaires were administered to 2866 early adolescents and their parents in China; structural equation modeling and bias-corrected bootstrap methods were used. The results show that different dimensions of parental involvement had different effects on mathematics achievement. Additionally, results indicate that the influences of the multidimensional construct of parental involvement on mathematics achievement were either partially or completely mediated by student’s mental health and mathematics self-efficacy. The findings also offer insight into possible interventions designed to explore how parental involvement promotes students’ mathematics achievement through their children’s mental health and mathematics self-efficacy.
210Po and 210Pb in King Bolete (Boletus edulis) and Related Mushroom Species: Estimated Effective Radiation Dose and Geospatial Distribution in Central and Eastern Europe
Authors: Strumińska-Parulska Dagmara , Moniakowska Aleksandra , Olszewski Grzegorz , Falandysz Jerzy
Published: September 2021

210Po and 210Pb occur naturally and are the most radiotoxic isotopes of the uranium (U) decay chain. Samples of Boletus edulis and related mushroom species, including B. pinophilus, B. reticulatus, B. luridus and B. impolitus, collected from Poland and Belarus were investigated for the activity concentrations of these isotopes and also for their potential health risk through adult human consumption. The results showed that spatially, the occurrence of 210Po and 210Po was heterogeneous, with activities varying from 0.91 to 4.47 Bq∙kg−1 dry biomass and from 0.82 to 5.82 Bq∙kg−1 db, respectively. Caps and stipes of the fruiting bodies showed similar levels of contamination. Consumption of boletes foraged in Poland could result in exposure to a combined radiation dose of 10 µSv∙kg−1 db from both isotopes. This dose is not significant compared to the total annual effective radiation dose of 210Po and 210Pb (54–471 µSv∙kg−1) from all sources, suggesting that these mushrooms are comparatively safe for human consumption.
PaLS Study of Sleep Deprivation and Mental Health Consequences of the COVID-19 Pandemic among University Students: A Cross-Sectional Survey
Authors: Gruba Grzegorz , Kasiak Przemysław Seweryn , Gębarowska Joanna , Adamczyk Natalia , Sikora Zuzanna , Jodczyk Alicja Monika , Mamcarz Artur , Śliż Daniel
Published: September 2021

The COVID-19 pandemic has changed the way many people live. To assess its impact on sleep quality and quantity, blue light exposure, and the mental health of Polish university students, a cross-sectional survey was conducted. Almost half of the participants were medical students (47.62%; n = 630). The majority of students were suffering from insomnia (58.13%, n = 769). Almost every third student was sleeping less than 7 h a day (30.39%, n = 402). Our study showed that a short sleep duration correlates with poorer mental health outcomes. Respondents who declared sadness and depression were more likely to suffer from insomnia (OR = 5.6997, 95% CI: 4.3641–7.4441). Difficulty with tasks was also more likely to co-occur with insomnia (OR = 5.4723, 95% CI: 4.3007 to 6.9630). The results of this study showed that the COVID-19 pandemic contributed to the deterioration of sleep quality and quantity as well as the psychological well-being of Polish students. It is important to take steps to promote proper sleeping habits to alleviate the risk of mental health disorders in this group of people.
Responses of Runoff and Soil Loss on Slopes to Land Use Management and Rainfall Characteristics in Northern China
Authors: Fang Haiyan
Published: September 2021

Soil conservation measures are widely used to control soil erosion and sediment loss; however, their proper usage relies on a deep understanding of the responses of runoff and sediment loss to land management and rainfall characteristics. In the present study, a long-term (2014–2020) monitored dataset derived from ten runoff plots in the upstream catchment of the Miyun Reservoir in Beijing, China, was used to study runoff and sediment loss responses to land use management and rainfall characteristics. The study results show that plots with no soil conservation measures had the highest runoff depth of 75 mm and suffered the highest sediment loss, at a rate of 3200 t km−2 yr−1. The terraced and vegetated plots generated lower runoff depths, with soil loss rates less than 213.0 t km−2 yr−1. With the exception of the contour tillage plots on steep slopes, the vegetation and engineering measures can efficiently reduce runoff and sediment loss, with both runoff and sediment reduction efficiencies higher than 76%. Statistical analyses indicate that, on the plots of bare soil and cultivation without soil conservation measures, runoff and sediment loss were mainly affected by the maximum 30 min rainfall intensity. However, on the plots with soil conservation measures, they were mainly determined by rainfall amount and duration. The sediment loss rate can be well fitted with the runoff depth using a power function. Based on the analyses, water-saving soil conservation measures are recommended for the study area. In addition, the size of terraces should be reconsidered on gentle slopes, and the coverage of forest, shrubs, and grass on slopes should be reduced, thus allowing for more surface runoff generation to ensure drinking water safety. In general, for the study area, soil conservation measures are required on the bare soil and cultivated slopes.
Assessing Concerns and Care Needs of Expectant Parents: Development and Feasibility of a Structured Interview
Authors: van Driessche Anne , van Stel Henk , Vink Remy , Staal Ingrid
Published: September 2021

Many adverse situations for parenting and healthy child development can be detected before a child’s birth. The aim of this project was to develop and test an instrument to use in prenatal home visits, to improve the identification of adverse situations and care needs during pregnancy. The preSPARK is based on a valid and reliable broad-scope structured interview called SPARK (Structured Problem Analysis of Raising Kids). The preSPARK focuses on 12 topics ranging from aspects of the period before pregnancy to future parents’ expectations. The preSPARK was tested in daily practice for feasibility and discriminative capacity. User experience was assessed from the perspective of the professional. In total, 64 home visits using the preSPARK were carried out by 21 nurses. About 24% of the expectant parents needed intensive help or immediate action on one or more topics. The risk assessment showed 29% of the participants were at high risk, 40% at increased risk, and 31% at low risk for future parenting and child developmental problems. The nurses indicated that the preSPARK provides a good structure for home visits and gives insight in interrelated factors. The preSPARK is feasible in daily practice and clarifies risks and care needs of expectant parents.
A Cross-Sectional Quantitative Study on Sexual and Reproductive Health Knowledge and Access to Services of Arab and Kurdish Syrian Refugee Young Women Living in an Urban Setting in Lebanon
Authors: Korri Rayan , Froeschl Guenter , Ivanova Olena
Published: September 2021

Since data on the sexual and reproductive health (SRH) of young refugee women living in urban settings in Lebanon are particularly scarce, we aim through this exploratory study to assess the SRH knowledge and access to services of Arab and Kurdish Syrian refugee young women living in Bourj Hammoud. From January to March 2020, a cross-sectional survey was conducted among 297 Syrian Arab and Kurdish participants and aged 18–30 years old. It was found that participants coming from Syrian urban areas or who completed an education above secondary level have higher overall knowledge on SRH issues. Only a total of 148 out of the 297 participants (49.8%) knew a health facility in Bourj Hammoud that provides SRH services and among them 36.4% did not know which type of services are available there. The Syrian refugee young women’s access to SRH services is inadequate due to different obstacles. The overall knowledge level on different SRH topics is limited. The context of multiple crises in Lebanon should be taken into consideration when delivering future SRH services.
Successful Catheter Ablation of the “R on T” Ventricular Fibrillation
Authors: Lasocka Zofia , Dąbrowska-Kugacka Alicja , Lewicka Ewa , Liżewska-Springer Aleksandra , Królak Tomasz
Published: September 2021

In patients with idiopathic ventricular fibrillation (VF), recurrent implantable cardioverter-defibrillator (ICD) shocks might increase mortality risk and reduce patients’ quality of life. Catheter ablation of triggering ectopic beats is considered to be an effective method. We present a patient with recurrent VF, caused by the “R on T” premature ventricular complexes. In the presented case radiofrequency catheter ablation efficiently eliminated arrhythmia trigger, which was possible to detect thanks to the intracardiac electrocardiograms (ECG’s) stored in the ICD.
Acute Effects of Intermittent Foot Cooling on 1 RM Leg Press Strength in Resistance-Trained Men: A Pilot Study
Authors: Wu Chih-Min , Lee Mei-Hsien , Wang Wen-Yi , Cai Zong-Yan
Published: September 2021

Inter-set peripheral cooling can improve high-intensity resistance exercise performance. However, whether foot cooling (FC) would increase 1 repetition maximum (RM) lower-limb strength is unclear. This study investigated the effect of intermittent FC on 1 RM leg press strength. Ten recreational male lifters performed three attempts of 1 RM leg press with FC or non-cooling (NC) in a repeated-measures crossover design separated by 5 days. FC was applied by foot immersion in 10 °C water for 2.5 min before each attempt. During the 1 RM test, various physiological measures were recorded. The results showed that FC elicited higher 1 RM leg press strength (Δ [95% CI]; Cohen’s d effect size [ES]; 13.6 [7.6–19.5] kg; ES = 1.631) and electromyography values in vastus lateralis (57.7 [8.1–107.4] μV; ES = 0.831) and gastrocnemius (15.1 [−3.1–33.2] μV; ES = 0.593) than in NC. Higher arousal levels (felt arousal scale) were found in FC (0.6 [0.1–1.2]; ES = 0.457) than in NC. In conclusion, the preliminary findings, although limited, suggest intermittent FC has a potential ergogenic role for recreational athletes to enhance maximal lower-limb strength and may partly benefit strength-based competition events.
The Influence of Technology on Mental Well-Being of STEM Teachers at University Level: COVID-19 as a Stressor
Authors: Navarro-Espinosa Johanna Andrea , Vaquero-Abellán Manuel , Perea-Moreno Alberto-Jesús , Pedrós-Pérez Gerardo , Aparicio-Martínez Pilar , Martínez-Jiménez Maria Pilar
Published: September 2021

Stress can result in psychopathologies, such as anxiety or depression, when this risk factor continues in time. One major stressor was the COVID-19 pandemic, which triggered considerable emotional distress and mental health issues among different workers, including teachers, with another stressor: technology and online education. A mixed-method approach is presented in this research, combining a cross-sectional study of university teachers from Ecuador and Spain with a medium of twenty years of working experience (N = 55) and a bibliometric analysis carried out in three databases (161 documents). The levels of anxiety and depression, and therefore the risk of developing them as mental disorders, were high. The lack of training (p < 0.01), time (p < 0.05), or research regarding the use of technology in education (p < 0.01) and stress caused by COVID-19 (p < 0.001) were linked to frequency. The most relevant observational study obtained through the bibliometric analysis (138 citations and over 65% of methodological quality) indicated that previous training and behavioral factors are key in the stress related to technology. The combination of the results indicated that mental health in STEM teachers at university is related to diverse factors, from training to the family and working balance.