bluuebunny/crossref_metadata_2025 huggingface dataset:
The Roots of Occasionalism? Causation, Metaphysical Dependence, and Soul-Body Relations in Augustine
Authors: Nawar Tamer
Published: November 2021

It has long been thought that Augustine holds that corporeal objects cannot act upon incorporeal souls. However, precisely how and why Augustine imposes limitations upon the causal powers of corporeal objects remains obscure. In this paper, the author clarifies Augustine’s views about the causal and dependence relations between body and soul. He argues that, contrary to what is often thought, Augustine allows that corporeal objects do act upon souls and merely rules out that corporeal objects exercise a particular kind of causal power (that of efficient or sustaining causes). He clarifies how Augustine conceives of the kind of causal influence exercised by souls and bodies.
Three new genera and new species of hystrignathid nematodes (Nematoda: Thelastomatoidea) from passalid beetles (Insecta: Passalidae) from Yunnan Province, China with phylogenetic analysis of Hystrignathidae
Authors: ZHANG NINGNING , YIN SHI , CARRENO RAMON A. , ZHANG LUPING
Published: November 2021

Three new genera and three new species of hystrignathid nematodes were collected from the hindgut of passalid beetles in the Yunnan Province, China. Huntia morffei gen. n. et sp. n. from Ceracupes fronticornis (Westwood) differs from related genera in the shape of the stoma, in the cephalic structure, and in the lack of annulation in the cervical region. Parakongonema sinica gen. n. et sp. n. from Aceraius grandis (Burmeister) and C. fronticornis can be easily distinguished from related genera in having the oral opening surrounded by a cuticular ring, the ring being surrounded by a cuticular flap, in the shape of the oesophagus, and in the cephalic structure. Parahystrignathus tongbiguanensis gen. n. et sp. n. from C. fronticornis and A. grandis differs from related genera by having the cervical region armed with alternating rows of spines, with 16 spines in the first row, and by lacking the first cephalic annule. In addition, the 18S and 28S rDNA partial sequences of three new species were obtained. The phylogeny of Hystrignathidae was inferred using partial sequences of the 28S rDNA and revealed patterns that suggest that the shape of the parasite digestive tract may reflect the evolutionary relationships of hystrignathid nematodes.
A novel design of wound bandage using heparin-polyvinylpyrrolidone/TiO2 nanocomposite to improved antibacterial treatment and burn wound healing effect: In vitro and in vivo evaluation
Authors: Li Xiuli , Wang Jigang , Li Xin , Hou Xiaoqian , Wang Hao , Li Hui , Zhang Chunhua
Published: November 2021

In our current study, porous heparin-polyvinylpyrrolidone/TiO2 nanocomposite (HpPVP/TiO2) bandage were prepared via the incorporation of TiO2 into HpPVP hydrogels for biomedical applications such as burn infection. The effect of the HpPVP hydrogels and
the nanoparticles of TiO2 composition on the functional group and the surface properties of the as-fabricated bandages were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray diffractometry (XRD). The presence of TiO2 nanoparticles created the internal
structure of the HpPVP hydrogel that aids in a homogeneous porous structure, as indicated by the scanning electron microscope (SEM). The size distribution of the TiO2 nanoparticles was measured using a transmission electron microscope (TEM). The studies on the mechanical properties
of the HpPVP hydrogel indicate that the addition of TiO2 nanoparticles increases its strength. The prepared HpPVP/TiO2 nanocomposite dressing has excellent antimicrobial activity were tested against bacterial species (Staphylococcus aureus and Escherichia coli)
and has good biocompatibility against human dermal fibroblast cells (HFFF2) for biological applications. In addition, in vivo evaluations in Kunming mice exposed that the as-fabricated HpPVP/TiO2 nanocomposite bandages increased the wound curing and facilitated accelerate
skin cell construction along with collagen development. The synergistic effects of the HpPVP/TiO2 nanocomposite hydrogel dressing material, such as its excellent hydrophilic nature, good bactericidal activity, biocompatibility and wound closure rate through in vivo test makes
it a suitable candidate for burn infections.
Regulation of melanoma cell growth by a miR-21 loaded targeted delivery system based on microparticles and nanoparticles targeting phosphatase and tensin homolog (PTEN)
Authors: Jin Lei , Yang Tong
Published: November 2021

The modulatory effect of miR-21 on the proliferation of melanoma cells through stimulation of PTEN (Phosphatase and tensin homologue deleted on chromosome 10) expression was investigated in the current study. PTEN, as a tumor suppressor, is expressed in low levels in melanoma tissues
and cell lines. Nevertheless, miR-21 can stimulate cancer development and suppress cell apoptosis. Overexpression of PTEN substantially impaired the proliferation of miR-21-treated melanoma cells. In addition, miR-21 and PTEN were observed to exhibit a combinatorial effect, whereas miR-21
could negatively regulate the expression of PTEN. In conclusion, these findings demonstrate that miR-21 affects melanoma development by targeting PTEN, establishing a new strategy for treating malignant melanoma. Furthermore, in this study, microparticles and nanoparticles were employed as
carriers to construct, through the self-assembly method, nanocapsules carrying miR-21 in order to develop an efficient nanocapsule delivery system of miR-21 against melanoma cells.
What led Asian American Buddhist background believers in Hawaii to become Christian: A grounded theory study
Authors: Hilderbrand Kelly Michael
Published: November 2021

The question asked of this grounded theory study was what are the perceived reasons Asian American Buddhist background believers in Hawaii converted to evangelical Christianity? The following factors were perceived as reasons for participants converting to Christianity: an exposure to Christianity, a crisis, a quest or search, and a divine connection—either emotional or miraculous. Studies on transformation or conversion have found a similar pattern (Mezirow, 1991; Rambo, 1993; Tippett, 1992; Ullman, 1989). However, more recent studies have identified an emotional or even supernatural component to conversion that needs to be explored further (Hilderbrand, 2016, 2020, 2021; Iyadurai, 2015; Snook et al., 2018). The outcome of conversion was expressed as a feeling of peace, joy and happiness.
Do Grittier People Have Greater Subjective Well-Being? A Meta-Analysis
Authors: Hou Xiang-Ling , Becker Nicolas , Hu Tian-Qiang , Koch Marco , Xi Ju-Zhe , Mõttus René
Published: November 2021

The present study conducted a meta-analysis to examine the relation between grit and subjective well-being (SWB). The association between grit (i.e., overall grit, perseverance of effort, and consistency of interest) and SWB (i.e., positive affect, negative affect, happiness, depression, life satisfaction, job satisfaction, and school satisfaction) were synthesized across 83 studies and 66,518 participants. The results based on a random-effects model showed a substantial correlation between overall grit and SWB (ρ = .46, 95% confidence interval [CI] = [.43, .48]), followed by perseverance of effort (ρ = .38, 95% CI = [.33, .43]) and consistency of interest (ρ = .23, 95% CI = [.17, .28]). The moderator analysis indicated that the correlations between overall grit/consistency of effort and SWB become weaker as age increased, and these links were stronger in affective well-being than in cognitive well-being. Moreover, grit explained unique variance in SWB even after controlling for conscientiousness. Implications and directions for further research are discussed.
Teaching musical learning as problem-solving: Applying a theory of musical intelligence to musical instruction
Authors: Sternberg Robert J , Kibelsbeck Jaime G
Published: November 2021

This article describes how a theory of musical intelligence can be applied to the teaching of music whereby musical learning is viewed as a form of problem-solving. We first introduce basic concepts and then describe the steps in a problem-solving cycle for musical learning. In particular, these steps involve recognizing the existence of a problem, defining the problem, allocating resources to the problem, mentally representing the problem, formulating a strategy to solve the problem, monitoring problem-solving, and evaluating the solution after one is done. Then, we discuss the application of these steps to analytical, creative, practical, and wisdom-based learning and performance. Finally, we draw some conclusions.
Return to Sports and Minimum 2-Year Outcomes of Hip Arthroscopy in Elite Athletes With and Without Coexisting Low Back Pain: A Propensity-Matched Comparison
Authors: Jimenez Andrew E. , Owens Jade S. , Monahan Peter F. , Maldonado David R. , Saks Benjamin R. , Sabetian Payam W. , Ankem Hari K. , Lall Ajay C. , Domb Benjamin G.
Published: November 2021

Background:

Patient-reported outcomes (PROs) and return to sports (RTS) after hip arthroscopy for femoroacetabular impingement syndrome (FAIS) have not been established in elite athletes with coexisting low back pain (LBP).

Purpose:

(1) To report minimum 2-year PROs and RTS rates after primary hip arthroscopy for FAIS in elite athletes with coexisting LBP and (2) to compare clinical results with a propensity-matched control group of elite athletes without back pain.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

Data were reviewed for elite athletes (college and professional) who underwent hip arthroscopy for FAIS and had coexisting LBP between October 2009 and October 2018. Inclusion criteria were preoperative and minimum 2-year follow-up for the modified Harris Hip Score, Nonarthritic Hip Score, Hip Outcome Score–Sports Specific Subscale (HOS-SSS), and visual analog scale for pain. Exclusion criteria were Tönnis grade >1, hip dysplasia (lateral center-edge angle <18°), and previous ipsilateral hip or spine surgery or conditions. Rates of achieving the minimal clinically importance difference (MCID), patient acceptable symptomatic state (PASS), and maximum outcome improvement satisfaction threshold were recorded in addition to RTS. For the subanalysis, the elite athlete study group was propensity matched to an elite athlete control group without back pain.

Results:

A total of 48 elite athletes with LBP who underwent primary hip arthroscopy met inclusion criteria, and follow-up was available for 42 (87.5%) at 53.2 ± 31.6 months (mean ± SD). Elite athletes with coexisting LBP demonstrated significant improvements in all recorded PROs and achieved the MCID and PASS for the HOS-SSS at rates of 82.5% and 67.5%, respectively. They also returned to sports at a high rate (75.8%), and 79% of them did not report LBP postoperatively. PROs, rates of achieving the MCID and PASS for the HOS-SSS, and RTS rates were similar between the study group and propensity-matched control group.

Conclusion:

Elite athletes with coexisting LBP who undergo primary hip arthroscopy for FAIS may expect favorable PROs, rates of achieving the MCID and PASS for the HOS-SSS, and RTS rates at minimum 2-year follow-up. These results were comparable to those of a propensity-matched control group of elite athletes without back pain. In athletes with hip-spine syndrome, successful treatment of their hip pathology may help resolve their back pain.
Modern pollen–climate relationships and their application for pollen-based quantitative climate reconstruction of the mid-Holocene on the southern Korean Peninsula
Authors: Lee Jaeyoung , Jun Chang-Pyo , Yi Sangheon , Kim Yongmi , Lee Eunmi , Kim Dongwook
Published: November 2021

A modern pollen dataset is a prerequisite for reconstructing quantitative paleoclimate and paleovegetation cover using fossil pollen records. Although multiple modern pollen–climate datasets have been established covering a wide range of climate conditions, such datasets are exceedingly rare for the Korean Peninsula (KP). In this study, we acquired a modern pollen dataset from 198 surface soil samples collected on 37 mountains on the southern KP. Redundancy analysis (RDA) and variation partitioning results identified mean annual temperature (Tann) as the most important climate variable shaping pollen assemblages on the southern KP. Using the pollen–climate relationships inferred from the RDA, we applied the Huisman–Olff–Fresco model and determined that arboreal pollen taxa responded sensitively to the climatic gradient, whereas non-arboreal pollen taxa did not. We applied weighted averaging–partial least squares (WA-PLS) and the modern analog technique (MAT) to the pollen dataset, and a comparison of the results showed that MAT performed better than WA-PLS. A transfer function was applied to fossil pollen records from the areas covered by our dataset; the results confirmed that annual precipitation (Pann) and Tann were modulated by different mechanisms, with Pann strongly affected by El Niño–Southern Oscillation-driven typhoons during the Holocene, whereas Tann was mainly influenced by the Tsushima Warm Current from 7500 to 5100 cal yr BP depending on Kuroshio Current inflow intensity, and subsequently followed by the East Asian winter monsoon during 5100–3400 cal yr BP.
Epicatechin ameliorative effects on methotrexate-induced hepatotoxicity in mice
Authors: Azadnasab Reza , Kalantar Hadi , Khorsandi Layasadat , Kalantari Heibatullah , Khodayar Mohammad Javad
Published: November 2021

Background

Due to the fact that methotrexate is widely used both as an immunosuppressive drug and as a chemotherapy agent, many studies are needed to reduce the side effects of this drug on non-target organs.

Purpose

This study was designed to investigate the effects of epicatechin (Epi) on MTX (methotrexate)-induced hepatotoxicity in mice.

Research Design

After 1 week for adaptation, we randomly divided 42 male Naval Medical Research Institute mice into six groups: (I) control; (II) Epi (100 mg/kg, po); (III) MTX (20 mg/kg, i.p.) on the fifth day; and (IV, V, and VI) Epi (25, 50, and 100 mg/kg, po) + MTX (20 mg/kg, i.p.) on the fifth day. At day 10, the mice were sacrificed and serum factors, oxidative stress markers, and inflammatory cytokines were measured.

Results

MTX increased activity level of serum enzymes (alanine aminotransferase and aspartate aminotransferase), lipid peroxidation marker (malondialdehyde), and inflammatory factors including interleukin-1 beta, tumor necrosis factor-alpha, and nitric oxide. Furthermore, MTX decreased glutathione level and activity level of catalase, superoxide dismutase, and glutathione peroxidase. Epi was able to reduce the destructive effects of oxidative/antioxidant system imbalance and inflammatory reactions and also histopathological damage in MTX intoxicated mice. Epi pretreatment reduced liver dysfunction by improving the antioxidant defense system, anti-inflammatory effects, and alleviation of histopathological damage in MTX hepatotoxicity.

Conclusions

Accordingly, Epi can be used as a therapeutic agent in hepatotoxicity associated with MTX chemotherapy.
Mycotic Pseudotumor of the Breast Secondary to Cryptococcal Infection: Report of Three Rare Cases and Literature Review
Authors: Makhubela Velaphi Glenda , Khaba Moshawa Calvin
Published: November 2021

Breast masses in clinical practice are often investigated primarily for neoplastic conditions. Breast fungal infections are unusual, and few cases have been reported in the literature. The differential diagnosis for a breast mass should not be limited to neoplastic conditions as there are treatment implications. The correct diagnosis is associated with reduced and unwanted cases of surgical intervention. We describe 3 cases of cryptococcal infection of the breast that clinically masqueraded as breast malignancies.
To Uncertainty and Beyond: Identifying the Capabilities Needed by Hospitals to Function in Dynamic Environments
Authors: Gifford Rachel , Fleuren Bram , van de Baan Frank , Ruwaard Dirk , Poesen Lieze , Zijlstra Fred , Westra Daan
Published: November 2021

Hospitals operate in increasingly complex and dynamically uncertain environments. To understand how hospital organizations can cope with such profound uncertainty, this article presents a multiple case study of five hospitals during the COVID-19 crisis in a heavily hit region of the Netherlands. We find that hospitals make adaptations in five key categories, namely: reorganization, decision-making, human resources, material resources, and planning. These adaptations offer insights into the core capabilities needed by hospitals to cope with dynamic uncertainty. Our findings highlight the need for hospitals to become more flexible without sacrificing efficiency. Organizations can accomplish this by building in more sensing and seizing capabilities to be better prepared for and respond to environmental change. Furthermore, transforming capabilities allow organizations to be more resilient and responsive in the face of ongoing uncertainty. We make recommendations on how hospitals can build these capabilities and address the core challenges they face in this pursuit.
Numerical simulation of the thermomechanical buckling analysis of bidirectional porous functionally graded plate using collocation meshfree method
Authors: Kumar Rahul , Lal Achchhe , Singh Bhrigu Nath , Singh Jeeoot
Published: November 2021

This paper presents some new and valuable numerical results for the thermo-mechanical buckling analysis of bidirectional porous functionally graded plates with uniform and non-uniform temperature rise. The strong form formulation is implemented for thermo-mechanical buckling in the framework of higher-order shear deformation theory. The material property with four schemes of porosity distribution of bidirectional porous functionally graded plate is taken by a modified power law. The governing differential equations are accomplished utilizing the principle of virtual works. The multi-quadric radial basis function is implemented for discretizing the governing differential equations. The multi-quadric radial basis function Euclidean norm is modified to analyze the square as well as rectangular plates without changing the shape parameters. Convergence and validation studies are performed to show the accuracy, effectiveness, and consistency of the present meshfree collocation method. The influence of different porosity distributions, span to thickness ratios, aspect ratios, grading index, temperature raise, boundary conditions, and porosity index on thermomechanical buckling load is evaluated. Some novel results for the bidirectional porous functionally graded plate are also enumerated that can be utilized as benchmark results for future reference.
Development of optimal diesel injection strategy based on the prediction of performance and emissions using deep neural network
Authors: Güner Erdoğan , Kaleli Aliriza , Bakirci Kadir , Ceviz Mehmet Akif
Published: November 2021

This study aims to determine the optimal injection strategy by predicting the performance and exhaust emission parameters of a four-cylinder CRDI engine under several operating conditions. The experimental determination procedure is challenging and expensive calibration task since it requires a high number of tests. Many studies have focused on a limited level of parameters. In this study, design of experiments technique and deep neural network (DNN) modeling are used together. The experimental data set for the model is created using Taguchi L16 and L32 orthogonal arrays. The DNN model is developed to predict [Formula: see text], [Formula: see text], HC, and CO emissions with speed, torque, injection timings and fuel quantities of each injection called as pilot1, pilot2, main, and post. In this way, it has become possible to evaluate the effects of a larger number of operating parameters in a wide range than the literature. The developed DNN model predicts the [Formula: see text], [Formula: see text], HC, and CO with R2 0.939, 0.943, 0.963, and 0.966, respectively. Additionally, RMSE and MAE values for the model are between 0.024 and 0.048. The proposed method compared with the conventional look-up table method performs better in reducing the complexity and cost of experiments and exploration of the effects of injection parameters on engine emission and performance characteristics in a wide engine operating range. In conclusion, until 2300 rpm at specified torque (90 Nm), it is found that 70% of fuel quantity should inject in main injection to minimize [Formula: see text] and [Formula: see text] emissions. The post injection quantity should be increased by reducing the amount of main injection from this operating condition on. Furthermore, it is observed that the ratios of pilot injection durations do not change with increasing engine speed, but quantity of first pilot injection is more than that of second pilot injection.
A Modular Genre? Problems in the Reception of the Post-Miyazaki ‘Ghibli Film’
Authors: Mes Tom , Agnoli Francis M
Published: November 2021

With the eternally looming spectre of Miyazaki Hayao’s retirement, the death of Takahata Isao and the failure to establish a viable new artistic figurehead to follow in their footsteps, Studio Ghibli has been at a crucial crossroads for some time. Over the past few decades, the acclaimed Japanese animation studio has adopted three main strategies to cope with these changes: apprenticeship to foster new talent, co-productions both domestically and abroad, and shutting down their production facilities. Each approach has affected Ghibli’s evolving brand identity – and the meaning of the ‘Ghibli film’ – causing confusion in the international critical reception of the resulting movies. Academic approaches too have shown difficulties dealing with recent shifts. While conceptualizing the ‘Ghibli film’ as the product of a studio brand or as the work of auteurs Miyazaki and Takahata has proven useful, such frameworks have become inadequate for accommodating these changes. This article therefore proposes a new approach for understanding recent ‘Ghibli films’, arguing that, rather than being treated as a brand or genre, they have increasingly been fashioned along modular lines.
Toward resourcefulness: pathways for community positive health
Authors: Peters Laura E. R. , Shannon Geordan , Kelman Ilan , Meriläinen Eija
Published: November 2021

Communities are powerful and necessary agents for defining and pursuing their health, but outside organizations often adopt community health promotion approaches that are patronizing and top-down. Conversely, bottom-up approaches that build on and mobilize community health assets are often critiqued for tasking the most vulnerable and marginalized communities to use their own limited resources without real opportunities for change. Taking into consideration these community health promotion shortcomings, this article asks how communities may be most effectively and appropriately supported in pursuing their health. This article reviews how community health is understood, moving from negative to positive conceptualizations; how it is determined, moving from a risk-factor orientation to social determination; and how it is promoted, moving from top-down to bottom-up approaches. Building on these understandings, we offer the concept of ‘resourcefulness’ as an approach to strengthen positive health for communities, and we discuss how it engages with three interrelated tensions in community health promotion: resources and sustainability, interdependence and autonomy, and community diversity and inclusion. We make practical suggestions for outside organizations to apply resourcefulness as a process-based, place-based, and relational approach to community health promotion, arguing that resourcefulness can forge new pathways to sustainable and self-sustaining community positive health.
GM-CSF secreting leukemia cell vaccination for MDS/AML after allogeneic HSCT: a randomized, double-blinded, phase 2 trial
Authors: Ho Vincent T. , Kim Haesook T. , Brock Jennifer , Galinsky Ilene , Daley Heather , Reynolds Carol , Weber Augustine , Pozdnyakova Olga , Severgnini Mariano , Nikiforow Sarah , Cutler Corey , Koreth John , Alyea Edwin P. , Antin Joseph H. , Gooptu Mahasweta , Romee Rizwan , Shapiro Roman , Chen Yi-Bin , Rosenblatt Jacalyn , Avigan David , Hodi F. Stephen , Dranoff Glenn , Wu Catherine J. , Ritz Jerome , Soiffer Robert J.
Published: November 2021

Vaccination using irradiated, adenovirus transduced autologous myeloblasts to secrete granulocyte–macrophage colony-stimulating factor (GVAX) early after allogeneic hematopoietic stem cell transplantation (HSCT) can induce potent immune responses. We conducted a randomized phase 2 trial of GVAX after HSCT for myelodysplastic syndrome with excess blasts or relapsed/refractory acute myeloid leukemia. Myeloblasts were harvested before HSCT to generate the vaccine. Randomization to GVAX vs placebo (1:1) was stratified according to disease, transplant center, and conditioning. Graft-versus-host disease (GVHD) prophylaxis included tacrolimus and methotrexate. GVAX or placebo vaccination was started between day 30 and 45 if there was engraftment and no GVHD. Vaccines were administered subcutaneously/intradermally weekly × 3, then every 2 weeks × 3. Tacrolimus taper began after vaccine completion. A total of 123 patients were enrolled, 92 proceeded to HSCT, and 57 (GVAX, n = 30; placebo, n = 27) received at least 1 vaccination. No Common Toxicity Criteria grade 3 or worse vaccine-related adverse events were reported, but injection site reactions were more common after GVAX (10 vs 1; P = .006). With a median follow-up of 39 months (range, 9-89 months), 18-month progression-free survival, overall survival, and relapse incidence were 53% vs 55% (P = .79), 63% vs 59% (P = .86), and 30% vs 37% (P = .51) for GVAX and placebo, respectively. Nonrelapse mortality at 18 months was 17% vs 7.7% (P = .18), grade II to IV acute GVHD at 12 months was 34% vs 12% (P = .13), and chronic GVHD at 3 years was 49% vs 57% for GVAX and placebo (P = .26). Reconstitution of T, B, and natural killer cells was not decreased or enhanced by GVAX. There were no differences in serum major histocompatibility chain-related protein A/B or other immune biomarkers between GVAX and placebo. GVAX does not improve survival after HSCT for myelodysplastic syndrome/acute myeloid leukemia. This trial was registered at www.clinicaltrials.gov as #NCT01773395.
The evolution of the metazoan Toll receptor family and its expression during protostome development
Authors: Orús-Alcalde Andrea , Lu Tsai-Ming , Børve Aina , Hejnol Andreas
Published: November 2021

Background

Toll-like receptors (TLRs) play a crucial role in immunity and development. They contain leucine-rich repeat domains, one transmembrane domain, and one Toll/IL-1 receptor domain. TLRs have been classified into V-type/scc and P-type/mcc TLRs, based on differences in the leucine-rich repeat domain region. Although TLRs are widespread in animals, detailed phylogenetic studies of this gene family are lacking. Here we aim to uncover TLR evolution by conducting a survey and a phylogenetic analysis in species across Bilateria. To discriminate between their role in development and immunity we furthermore analyzed stage-specific transcriptomes of the ecdysozoansPriapulus caudatusandHypsibius exemplaris, and the spiraliansCrassostrea gigasandTerebratalia transversa.

Results

We detected a low number of TLRs in ecdysozoan species, and multiple independent radiations within the Spiralia. V-type/scc and P-type/mcc type-receptors are present in cnidarians, protostomes and deuterostomes, and therefore they emerged early in TLR evolution, followed by a loss in xenacoelomorphs. Our phylogenetic analysis shows that TLRs cluster into three major clades: clade α is present in cnidarians, ecdysozoans, and spiralians; clade β in deuterostomes, ecdysozoans, and spiralians; and clade γ is only found in spiralians. Our stage-specific transcriptome and in situ hybridization analyses show that TLRs are expressed during development in all species analyzed, which indicates a broad role of TLRs during animal development.

Conclusions

Our findings suggest that a clade α TLR gene (TLR-Ca) and a clade β/γ TLR gene (TLR-Cβ/γ) were already present in the cnidarian-bilaterian common ancestor. However, althoughTLR-Cawas conserved in cnidarians,TLR-Cβ/γwas lost during the early evolution of these taxa. Moreover,TLR-Cβ/γduplicated to generateTLR-CβandTLR-Cγin the lineage to the last common protostome-deuterostome ancestor.TLR-Ca, TLR-CβandTLR-Cγfurther expanded generating the three major TLR clades. While all three clades radiated in several spiralian lineages, specific TLRs clades have been presumably lost in other lineages. Furthermore, the expression of the majority of these genes during protostome ontogeny suggests a likely role in development.
The earliest beetle with mouthparts specialized for feeding on nectar is a parasitoid of mid-Cretaceous Hymenoptera
Authors: Batelka Jan , Prokop Jakub
Published: November 2021

Background

During the Mesozoic, there were many insects in several holometabolous orders (Neuroptera, Mecoptera and Diptera) with elongated mouthparts adapted for feeding on nectar. The evolutionary history of the megadiverse order Coleptera, which has a great diversity of mouthparts and feeding strategies, is well documented since early Permian with a significant peak in diversity in the Triassic. Currently, however, there is no evidence that in the Mesozoic these beetles fed on nectar despite the recorded specializations for pollination of flowering plants in several families since the mid-Cretaceous.

Results

Here we describe a new wedge-shaped beetle Melanosiagon serraticornis gen. et sp. nov. from mid-Cretaceous Burmese amber attributed to Macrosiagonini (Ripiphoridae: Ripiphorinae), which has elongated galea comparable to that in the extant parasitoid genus Macrosiagon, and a well known example of adaptation for nectar feeding in Coleoptera. Furthermore, Salignacicola gen. nov. is established for Macrosiagon ebboi Perrichot, Nel et Néraudeau, 2004, based on the holotype found in mid-Cretaceous amber from France. Systematic positions of both newly established genera are discussed. A list of potential wasp and bee hosts of Ripiphorinae from the Mesozoic is provided.

Conclusions

This study presents evidence of the earliest occurrence of specialized nectar feeding mouthparts in Coleoptera. Melanosiagon serraticornis is closely related to extant Macrosiagonini. In all genera belonging to subfamily Ripiphorinae the primary larvae are adapted for parasitism on aculeate Hymenoptera (bees and wasps) and adults are associated with blossoms of flowering plants, in terms of their specialized morphology. Adults of Macrosiagon visit blossoms of flowering plants to obtain nectar and lay eggs from which the hatching larvae attack visiting wasps and bees. An association with flowers of some tropical trees is already corroborated in some extant species. Interestingly the larvae of Ripiphorinae are also found in Burmese amber. Thus, both life stages of the mid-Cretaceous Ripiphorinae indicate a close association of this lineage with flowering trees.
Integrative transcriptomic and metabolomic analysis of D-leaf of seven pineapple varieties differing in N-P-K% contents
Authors: Chen Jing , Zeng Hui , Zhang Xiumei
Published: November 2021

Background

Pineapple (Ananas comosus L. Merr.) is the third most important tropical fruit in China. In other crops, farmers can easily judge the nutritional requirements from leaf color. However, concerning pineapple, it is difficult due to the variation in leaf color of the cultivated pineapple varieties. A detailed understanding of the mechanisms of nutrient transport, accumulation, and assimilation was targeted in this study. We explored the D-leaf nitrogen (N), phosphorus (P), and potassium (K) contents, transcriptome, and metabolome of seven pineapple varieties.

Results

Significantly higher N, P, and K% contents were observed in Bali, Caine, and Golden pineapple. The transcriptome sequencing of 21 libraries resulted in the identification of 14,310 differentially expressed genes in the D-leaves of seven pineapple varieties. Genes associated with N transport and assimilation in D-leaves of pineapple was possibly regulated by nitrate and ammonium transporters, and glutamate dehydrogenases play roles in N assimilation in arginine biosynthesis pathways. Photosynthesis and photosynthesis-antenna proteins pathways were also significantly regulated between the studied genotypes. Phosphate transporters and mitochondrial phosphate transporters were differentially regulated regarding inorganic P transport. WRKY, MYB, and bHLH transcription factors were possibly regulating the phosphate transporters. The observed varying contents of K% in the D-leaves was associated to the regulation of K+ transporters and channels under the influence of Ca2+ signaling. The UPLC-MS/MS analysis detected 873 metabolites which were mainly classified as flavonoids, lipids, and phenolic acids.

Conclusions

These findings provide a detailed insight into the N, P, K% contents in pineapple D-leaf and their transcriptomic and metabolomic signatures.
Randomized trial examining the effect of exercise and wellness interventions on preventing postpartum depression and perceived stress
Authors: Lewis Beth A. , Schuver Katie , Dunsiger Shira , Samson Lauren , Frayeh Amanda L. , Terrell Carrie A. , Ciccolo Joseph T. , Fischer John , Avery Melissa D.
Published: November 2021

Background

Approximately 13–19% of postpartum women experience postpartum depression and a majority report at least some stress during the postpartum phase. Traditional interventions such as psychotherapy and antidepressant medications are often not feasible or desirable. The purpose of this study was to examine two low cost, brief, accessible interventions designed to prevent postpartum depression and perceived stress among women at high risk.

Methods

Participants (n = 450) who were on average four weeks postpartum, had a history of depression before pregnancy, and exercised less than 60 min per week were randomly assigned to one of the following three conditions: (1) 6-month telephone-based exercise intervention; (2) 6-month telephone-based wellness/support intervention (e.g., healthy eating, sleep, and perceived stress); or (3) usual care.

Results

Overall, 2.4% of participants met criteria for depression at 6 months and 3.6% at 9 months with no differences between groups. At 6 months following randomization, median symptoms of depression were significantly lower among wellness participants compared to usual care participants (b = − 1.00, SE = 0.46, p = .03). Perceived stress at 6 months post-randomization was significantly lower among exercise vs. usual care participants (b = − 2.00, SE = .98, p = .04) and exercise vs. wellness participants (b = − 2.20, SE = 1.11, p = .04).

Conclusions

The wellness intervention was efficacious for preventing symptoms of depression; however, postpartum depression that met the diagnostic criteria was surprisingly low in all conditions among this at risk sample of postpartum women. Exercise interventions may have a protective effect on perceived stress among women at risk for postpartum depression. Practitioners should consider integrating exercise and wellness interventions into postpartum care.

Trial registration

Clinical Trials Number: NCT01883479 (06/21/2013).
Exploring the association between use of gonadotropin releasing hormones agonists and prostate cancer diagnosis per se and diabetes control in men with type 2 diabetes mellitus: a nationwide, population-based cohort study
Authors: Lin E. , Garmo Hans , Van Hemelrijck Mieke , Adolfsson Jan , Stattin Pär , Zethelius Björn , Crawley Danielle
Published: November 2021

Background

Gonadotropin Releasing Hormones agonists (GnRH), which are first line treatment for metastatic prostate cancer (PCa), increase risk of type 2 diabetes mellitus (T2DM). This study aims to quantify the association of use of GnRH with diabetes control in PCa men with T2DM.

Methods

Nationwide population-based cohort study in the Swedish National Diabetes Register and Prostate Cancer data Base Sweden 4.1, on the association between GnRH and diabetes control in T2DM men with PCa by comparing T2DM men with PCa vs. without PCa, as well as comparing T2DM men with PCa on or not on GnRH. The primary exposure was use of GnRH. Worsening diabetes control was the primary outcome, defined as: 1) HbA1c rose to 58 mmol/mol or higher; 2) HbA1c increase by 10 mmol/mol or more; 3) Start of antidiabetic drugs or switch to insulin. We also combined all above definitions. Cox proportional hazards regression was used to analyze the association.

Results

There were 5714 T2DM men with PCa of whom 692 were on GnRH and 28,445 PCa-free men with T2DM with similar baseline characteristics. Diabetes control was worse in men with GnRH vs. PCa-free men (HR: 1.24, 95% CI: 1.13–1.34) as well as compared with PCa men without GnRH (HR:1.58, 95% CI: 1.39–1.80), when we defined the worsening control of diabetes by combining all definitions above.

Conclusion

Use of GnRH in T2DM men with PCa was associated with worse glycemic control. The findings highlight the need to closely monitor diabetes control in men with T2DM and PCa starting GnRH.
Predictors of visual recovery in patients with macular edema secondary to central retinal vein occlusion after treatment with Conbercept
Authors: Zhang Qi , Hou Yinfen , Cao Xiao , Zhang Rongrong , Liu Yinping , Wei Chenghua , Wu Changfan , Mei Lixin , Zhang Pengfei
Published: November 2021

Background

The use of Spectral domain optical coherence tomography (SD-OCT) to evaluate the predictors of visual acuity-recovery in patients treated with conbercept for macular edema (ME) secondary to central retinal vein occlusion (CRVO) has rarely been seen. We collected 26 CRVO-ME patients with different OCT measures at 6 months follow-up to identify the factors that are most strongly correlated with the best-corrected visual acuity (BCVA) post-treatment in CRVO-ME patients treated with conbercept.

Purpose

To evaluate the effectiveness of intravitreal conbercept injections for the treatment of CRVO-ME and to determine the major predictors of best-corrected visual acuity (BCVA) post-treatment.

Methods

A retrospective study methodology was used. Twenty-six eyes from 26 patients with CRVO-ME were enrolled in the study. After an initial intravitreal injection of conbercept (0.5 mg/0.05 ml), monthly injections for up to 6 months were given following a 1 + PRN (pro re nata) regimen. Data collected at monthly intervals included measurements of the logMAR BCVA, central subfield thickness (CST), macular volume (MV), photoreceptor layer thickness (PLT), outer nuclear layer thickness (ONLT), and the disrupted ellipsoid zone (DEZ). The correlation between BCVA, before and after injections, and each of CST, MV, PLT, ONLT, DEZ was analyzed.

Results

The logMAR BCVA in months 3 and 6 post-injection was significantly improved relative to the baseline. In this same period the CST, MV, PLT, ONLT and DEZ were also significantly improved relative to the baseline. There was a negative correlation between PLT and logMAR BCVA at months 3 and 6 after treatment (r = − 0.549, P < 0.001; r = − 0.087, P < 0.001).

Conclusion

Intravitreal injection of conbercept is an effective treatment for CRVO-ME. With 6 months of follow-up, logMAR BCVA and CST, MV, PLT, ONLT, DEZ improved. PLT was negatively correlated with the visual function in CRVO-ME patients after conbercept treatment, which may be a predictor of vision recovery in patients with CRVO-ME.
Transmission of cytomegalovirus via breast milk in low birth weight and premature infants: a systematic review and meta-analysis
Authors: Hu Xiaolin , Hu Wei , Sun Xuan , Chen Ling , Luo Xiaoping
Published: November 2021

Background

This study aimed to investigate the transmission of cytomegalovirus (CMV) via breast milk in low birth weight (LBW) and premature infants and its effects.

Methods

PubMed, Medline, Cochrane Library, and Embase were searched for studies (without language and time restriction) published before March 27, 2020, that examined the effect of CMV transmitted by breast milk on LBW and premature infants. The rates of breast milk-acquired CMV infection, CMV-related symptoms, and CMV-related sepsis-like syndrome (CMV-SLS) in LBW and premature infants were pooled from each study.

Results

Eighteen studies with 1920 LBW and premature infants were included. The pooled CMV infection rate from breast milk for infants fed untreated breast milk was significantly higher than those fed frozen breast milk [19.3, 95% confidence interval (CI) = 11.8–29.9% vs. 13.5, 95% CI = 8.0–22.0%,P < 0.01). Similarly, the pooled CMV infection rate for infants fed untreated breast milk was significantly higher than those with mixed feeding (P < 0.0001). The mixed feeding group had a significantly lower rate of CMV-related symptoms than the other groups (2.4%,P < 0.01).

Conclusions

These findings suggested a higher CMV infection rate in LBW or premature infants fed untreated breast milk than other feeding groups. Studies on the long-term outcomes of CMV infection transmitted from breast milk are needed to address the optimal feeding practice.
Displacement and stress distribution of the craniomaxillofacial complex under different surgical conditions: a three-dimensional finite element analysis of fracture mechanics
Authors: Chen Junjie , Xu Yuhan , Li Chengri , Zhang Lingling , Yi Fang , Lu Yanqin
Published: November 2021

Objective

To provide a simplified treatment strategy for patients with maxillary transverse deficiency. We investigated and compared the fracture mechanics and stress distribution of a midline palatal suture under dynamic loads during surgically-assisted rapid palatal expansion.

Methods

Based on the cone-beam computed tomography (CBCT) data of a 21-year-old female volunteer, a three-dimensional model of the cranio-maxillofacial complex (including the palatal suture) was constructed. A finite element analysis model was constructed based on meshwork. After the yield strength of the palatal suture was set, an increasing expansion force (0–500 N) was applied within 140 ms to calculate the time–load curve, which mimicked nonsurgical bone expansion (model A). The same method was used to evaluate the fracture process, time and stress distribution of the palatal suture in maxillary lateral osteotomy-assisted (model B) and LeFort osteomy I (LFIO)-assisted expansion of the maxillary arch (model C).

Results

Compared with model A, the palatal suture of model B and model C showed a faster stress accumulation rate and shorter fracture time, and the fracture time of model B and model C was almost identical. Compared with model A, we discovered that model B and model C showed greater lateral extension of the maxilla, and the difference was reflected mainly in the lower part of the maxilla, and there was no difference between model B and model C in lateral extension of the maxilla.

Conclusions

Compared with arch expansion using nonsurgical assistance (model A), arch expansion using maxillary lateral wall-osteotomy (model B) or LFIO had a faster rate of stress accumulation, shorter time of fracture of the palatal suture and increased lateral displacement of the maxilla. Compared with arch expansion using LFIO (model C), arch expansion using lateral osteotomy (model B) had a similar duration of palatal suture rupture and lateral maxillary extension. In view of the trauma and serious complications associated with LFIO, maxillary lateral wall-osteotomy could be considered a substitute for LFIO.
Enablers of the successful implementation of simulation exercises: a qualitative study among nurse teachers in undergraduate nursing education
Authors: Haddeland Kristine , Slettebø Åshild , Fossum Mariann
Published: November 2021

Background

Simulation exercises are increasingly being used as a teaching method in the field of undergraduate nursing education. Thus, the present study sought to identify, describe and discuss enablers of the successful implementation of simulation exercises in undergraduate nursing education.

Methods

This study had a qualitative descriptive design and involved individual interviews conducted between November and December 2018 with six nurse teachers from three different university campuses in Norway. The transcribed interviews were analysed by means of a qualitative thematic analysis.

Results

The majority of the interviewees wanted to offer more simulation exercises as part of their respective undergraduate nursing education programmes. Moreover, creating a safe environment, facilitating student-centred learning and promoting reflection were all identified by the interviewees as enablers of the successful implementation of simulation exercises.

Conclusions

The findings of this study indicate that nurse teachers consider simulation to be a valuable teaching method for improving students’ learning outcomes. In addition, the findings could guide the future implementation of simulation exercises in undergraduate nursing education.

Trial registration

ClinicalTrials.gov ID: NCT 04063319.

Protocol ID: 52110 Nursing Students’ Recognition of and Response to Deteriorating Patients.
Adolescents’ perceived barriers to accessing sexual and reproductive health services in California: a cross-sectional survey
Authors: Decker Martha J. , Atyam Tara V. , Zárate Catherine Gilmore , Bayer Angela M. , Bautista Consuelo , Saphir Melissa
Published: November 2021

Background

Adolescents may forego needed sexual and reproductive health (SRH) services due to a variety of concerns and barriers. The purpose of this study is to compare adolescents’ perceptions of these barriers by participant characteristics including race/ethnicity, gender, sexual orientation, housing situation, and sexual experience.

Methods

Adolescents in a California-wide sexual health education program completed an anonymous survey at baseline (N = 10,015) about perceived barriers to using SRH services. Logistic regression analyses that accounted for the clustered data structure assessed differences by gender, age, sexual orientation, race/ethnicity, living situation, and sexual experience.

Results

The majority of participants were Hispanic/Latino (76.4%) with an average age of 14.9 years, and 28.8% had sexual experience. Half of the youth reported concerns about test results (52.7%), cost of services (52.0%), and confidentiality of services (49.8%). When controlling for other characteristics, youth identifying as transgender/non-binary/multiple genders had the highest odds of perceiving cost (odds ratio (OR) 1.89) and confidentiality (OR 1.51) as barriers. Increasing age was associated with decreasing odds of all barriers. Sexual orientation was a consistent predictor, with LGBQ+ youth having higher odds of perceiving test results (OR 1.21), cost (OR 1.36), and confidentiality (OR 1.24) as barriers. Asian or Pacific Islander/Native Hawaiian youth had higher odds of perceiving test results (OR 1.68) and cost (OR 1.37) as barriers. In contrast, Black youth had lower odds of reporting cost (OR 0.65) and confidentiality (OR 0.77) as barriers. Younger respondents and youth who identified as female, transgender/non-binary/multiple genders, LGBQ+, and Asian or Pacific Islander/Native Hawaiian had higher odds of reporting five or more barriers compared to reference groups.

Conclusions

The majority of adolescents face barriers to accessing appropriate SRH services, with females, gender-minority youth, younger adolescents, LGBQ+ youth, and Asian and Pacific Islander/Native Hawaiian youth more likely than others to report barriers. Access to SRH services can be improved through strengthening linkages between clinics and SRH education programs, providing youth-friendly clinical services, and ensuring youth have sufficient information, skills, and support to access care.

Trial registration

Approved by California Health and Human Services Agency’s Committee for the Protection of Human Subjects [12-08-0658, 11/30/2017].