bluuebunny/crossref_metadata_2025 huggingface dataset:
An Analysis of Deterministic Chaos as an Entropy Source for Random Number Generators
Authors: Demir Kaya , Ergün Salih
Published: December 2018

This paper presents an analytical study on the use of deterministic chaos as an entropy source for the generation of random numbers. The chaotic signal generated by a phase-locked loop (PLL) device is investigated using numerical simulations. Depending on the system parameters, the chaos originating from the PLL device can be either bounded or unbounded in the phase direction. Bounded and unbounded chaos differs in terms of the flatness of the power spectrum associated with the chaotic signal. Random bits are generated by regular sampling of the signal from bounded and unbounded chaos. A white Gaussian noise source is also sampled regularly to generate random bits. By varying the sampling frequency, and based on the autocorrelation and the approximate entropy analysis of the resulting bit sequences, a comparison is made between bounded chaos, unbounded chaos and Gaussian white noise as an entropy source for random number generators.
Causality in Vietnam’s Parallel Exchange Rate System during 2005–2011: Policy Implications for Macroeconomic Stability
Authors: Bui Minh Tam
Published: December 2018

As in many transition economies, Vietnam has experienced a multiple exchange rate system with three exchange rates having co-existed. This paper uses the Vector-Error-Correction model and the Granger tests to investigate the relationship between the official and black market exchange rates from January 2005 to April 2011. The results confirm a long-run relationship between the official and parallel market rates of the Vietnam dong against the U.S. dollar. The short-run dynamics of two exchange rates suggest that the official exchange rate causes the black exchange rate, but not vice versa. This conclusion is valid for both a sub-period of stability and a sub-period of vibrant fluctuations, with February 2008 as the cut-off. The findings also reject the efficiency hypothesis of the black market for foreign exchange and support the policy choice of the State Bank of Vietnam not to follow black market signals in managing official exchange rates for macroeconomic stability.
Application of Artificial Neural Networks for Noise Barrier Optimization
Authors: Zannin Paulo Henrique Trombetta , Do Nascimento Eriberto Oliveira , Da Paz Elaine Carvalho , Do Valle Felipe
Published: December 2018

In the modern world, noise pollution continues to be a major problem that impairs people’s health, and road traffic is a primary contributor to noise emissions. This article describes an environmental impact study of the noise generated by the reconstruction of an urban section of a highway. Noise maps were calculated, and an environmental impact matrix was generated to determine the environmental impact of this reconstruction. The implementation of noise barriers was simulated based on these noise maps, and the effectiveness of the barriers was evaluated using Artificial Neural Networks (ANNs) combined with Design of Experiments (DoE). A functional variable significance analysis was then made for two parameters, namely, the coefficient of absorption of the barrier material and the barrier height. The aim was to determine the influence of these parameters on sound attenuation and on the formation of acoustic shadows. The results obtained from the ANNs and DoE were consistent in demonstrating that the absorption coefficient strongly influences the noise attenuation provided by noise barriers, while barrier height is correlated with the formation of larger areas of acoustic shadow. The environmental impact matrix also indicates that the existence of noise pollution has a negative effect on the environment, but that this impact can be reversed or minimized. The application of simulated noise barriers demonstrated that noise levels can be reduced to legally acceptable levels.
Origin and Background Estimation of Sulfur Dioxide in Ulaanbaatar, 2017
Authors: Prikaz Makhbal , Fang Chunsheng , Dash Sanchirbayar , Wang Ju
Published: December 2018

Particulate matter studies have been conducted regularly in the capital city of Mongolia. In contrast, studies related to the source and general estimation of levels of sulfur dioxide (SO2) over whole years are lacking. To explore the yearly trend in SO2, whole-year data of air pollutants were obtained from the Air Pollution Reducing Department. The results showed that the annual average concentration of SO2 was 32.43 µg/m3 at the Amgalan official monitoring station in 2017, which changed from 53 µg/m3 in 2016, representing a reduction of around 40%. The back-trajectory model and the National Oceanic and Atmospheric Administration (NOAA)’s hybrid single particle Lagrangian integrated trajectory model (HYPSLIT) were used to determine the source of SO2. A total of 8760 backward trajectories were divided into eight groups. The results showed that 78.8% of the total trajectories in Ulaanbaatar came from an area inside Mongolia. The results showed that pollutants enter Ulaanbaatar mainly from the northwest and north during the winter season. There are industrial cities, such as Darkhan and Sukhbaatar, in North Mongolia. Air pollutants created in the industrial area traveled into Ulaanbaatar during the winter season.
Magneto Rotational Instability in Magnetized AGN Tori
Authors: Kudoh Yuki , Wada Keiichi
Published: December 2018

It is widely believed that, in active galactic nuclei (AGNs), a supermassive black hole with an accretion disk is surrounded by an optically and geometrically thick torus at sub-parsec scale. However, it is not clear how the mass supply is toward the central engine caused and how it is related with the internal structures of the tori. The magnetic field in the tori may contribute to the accretion process via the magneto-rotational instability (MRI). Using global three-dimensional magnetohydrodynamic (MHD) simulations taking the effects of X-ray heating and radiative cooling into account studied the numerical resolution for azimuthal direction for MRI driving. We found that a strongly magnetized disk consisted of a cold (< 10 3 K) and warm ( 10 4 K) gas is developed in about 30 rotational periods. We also found in a high resolution model that the mean azimuthal magnetic fields reverse their direction quasi-periodically. We confirmed that the typical wave length of the MRI should be resolved with a least 20 azimuthal grid cells.
Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
Authors: Königer Annabella , Grath Sonja
Published: December 2018

Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regions. Phenotypic assays of chill coma recovery time (CCRT) together with previously published population genetic data suggest that only a small number of genes underlie improved cold hardiness in the cold-adapted populations. We used high-throughput RNA sequencing to analyze differential gene expression before and after exposure to a cold shock in coldtolerant lines (those with fast chill coma recovery, CCR) and cold-sensitive lines (slow CCR) from a population originating from Bangkok, Thailand (the ancestral species range). We identified two candidate genes with a significant interaction between cold tolerance and cold shock treatment: GF14647 and GF15058. Further, our data suggest that selection for increased cold tolerance did not operate through the increased activity of heat shock proteins, but more likely through the stabilization of the actin cytoskeleton and a delayed onset of apoptosis.
Editorial for Quantitative Geomorphology Special Issue
Authors: Gioia Dario
Published: December 2018

In recent years, DEM- and GIS-supported analysis of landscape has become an important research field in many geomorphological applications, which aim to model surface processes in a variety of geomorphic environments and at different spatial and temporal scales. [...]
Relative Density of Away from Home Food Establishments and Food Spend for 24,047 Households in England: A Cross-Sectional Study
Authors: Penney Tarra L. , Burgoine Thomas , Monsivais Pablo
Published: December 2018

Eating away from home is a risk factor for poor diet quality and obesity. With an ever-increasing proportion of household food spend directed toward eating out, the proliferation of these food establishments may contribute to their use, a potential precursor to less healthy food choices and low overall diet quality. However few studies are conducted at the national level and across a range of away from home food sources. The purpose of this study was to examine the association between the density of away from home food establishments (e.g., restaurants, fast food outlets and cafés) and household spend on away from home food within a nationally representative sample for England, UK. A cross-sectional analysis of data from Wave 1 of the UK Household Longitudinal Survey (n = 24,047 adults aged ≥19y) was conducted. Exposure was characterised as the density of away from home food establishments to all other food sources within 1 mile of the home, divided into quintiles (Q1 as lowest exposure and Q5 as highest exposure). The primary outcome included households with a high away from home equivalised monthly food spend (≥25% of total food spend). Logistic regression was used to estimate associations between away from home food establishment exposure and high away from home food spend. Away from home food establishment density was significantly associated with a greater odds of high monthly food spend (Q3: OR = 1.18, 95% CI = 1.07, 1.30; Q4: OR = 1.30, 95% CI = 1.18, 1.43; and Q5: OR = 1.52, 95% CI = 1.37, 1.68) with attenuation after controlling for known socioeconomic confounders (Q4: OR = 1.13, 95% CI = 1.02, 1.25; and Q5: OR = 1.16, 95% CI = 1.04, 1.30) compared to those least exposed (Q1). Those most exposed to away from home food establishments had a 16% greater odds of allocating more than 25% of household food spend on away from home food sources. This study provides one of the first analyses at the national level to examine the role of the local food environment in relation to household food spend, a potential precursor to diet quality and health.
The New International Journal of Environmental Research and Public Health (IJERPH) Policy Concerning Tobacco Company Funding
Authors:   The IJERPH Editorial Office
Published: December 2018

IJERPH is proud to announce that it is adopting a new policy concerning the publication of research funded by tobacco companies. [...]
Geoinformatics in Citizen Science
Authors: Bordogna Gloria
Published: December 2018

This editorial introduces the special issue entitled “Geoinformatics in Citizen Science” of the ISPRS International Journal of Geo-Information. The issue includes papers dealing with three main topics. (1) Key tasks of citizen science (CS) in leveraging geoinformatics. This comprises descriptions of citizen science initiatives where geoinformation management and processing is the key means for discovering new knowledge, and it includes: (i) “hackAIR: Towards Raising Awareness about Air Quality in Europe by Developing a Collective Online Platform” by Kosmidis et al., (ii) “Coupling Traditional Monitoring and Citizen Science to Disentangle the Invasion of Halyomorpha halys” by Malek et al., and (iii) “Increasing the Accuracy of Crowdsourced Information on Land Cover via a Voting Procedure Weighted by Information Inferred from the Contributed Data” by Foody et al. (2) Evaluations of approaches to handle geoinformation in CS. This examines citizen science initiatives which critically analyze approaches to acquire and handle geoinformation, and it includes: (iv) “CS Projects Involving Geoinformatics: A Survey of Implementation Approaches” by Criscuolo et al., (v) “Obstacles and Opportunities of Using a Mobile App for Marine Mammal Research” by Hann et al., (vi) “OSM Data Import as an Outreach Tool to Trigger Community Growth? A Case Study in Miami” by Juhász and Hochmair, and (vii) “Experiences with Citizen-Sourced VGI in Challenging Circumstances“ by Hameed et al. (3) Novel geoinformatics research issues: (viii) “A New Method for the Assessment of Spatial Accuracy and Completeness of OpenStreetMap Building Footprints” by Brovelli and Zamboni, (ix) “A Citizen Science Approach for Collecting Toponyms” by Perdana and Ostermann, and (x) “An Automatic User Grouping Model for a Group Recommender System in Location-Based Social Networks” by Khazaei and Alimohammadi.
A Framework for Visual Analytics of Spatio-Temporal Sensor Observations from Data Streams
Authors: Sibolla Bolelang H. , Coetzee Serena , Van Zyl Terence L.
Published: December 2018

Sensor networks generate substantial amounts of frequently updated, highly dynamic data that are transmitted as packets in a data stream. The high frequency and continuous unbound nature of data streams leads to challenges when deriving knowledge from the underlying observations. This paper presents (1) a state of the art review into visual analytics of geospatial, spatio-temporal streaming data, and (2) proposes a framework based on the identified gaps from the review. The framework consists of (1) the data model that characterizes the sensor observation data, (2) the user model, which addresses the user queries and manages domain knowledge, (3) the design model, which handles the patterns that can be uncovered from the data and corresponding visualizations, and (4) the visualization model, which handles the rendering of the data. The conclusion from the visualization model is that streaming sensor observations require tools that can handle multivariate, multiscale, and time series displays. The design model reveals that the most useful patterns are those that show relationships, anomalies, and aggregations of the data. The user model highlights the need for handling missing data, dealing with high frequency changes, as well as the ability to review retrospective changes.
Hypothesis: Spontaneous Advent of the Prebiotic Translation System via the Accumulation of L-Shaped RNA Elements
Authors: Agmon Ilana
Published: December 2018

The feasibility of self-assembly of a translation system from prebiotic random RNA chains is a question that is central to the ability to conceive life emerging by natural processes. The spontaneous materialization of a translation system would have required the autonomous formation of proto-transfer RNA (tRNA) and proto-ribosome molecules that are indispensable for translating an RNA chain into a polypeptide. Currently, the vestiges of a non-coded proto-ribosome, which could have only catalyzed the formation of a peptide bond between random amino acids, is consensually localized in the region encircling the peptidyl transferase center of the ribosomal large subunit. The work presented here suggests, based on high resolution structures of ribosomes complexed with messenger RNA (mRNA) and tRNAs, that three types of L-shaped RNA building blocks derived from the modern ribosome, alongside with an L-shaped proto-tRNA, each composed of about 70-mer, could have randomly occurred in the prebiotic world and combined to form a simple translation system. The model of the initial coded proto-ribosome, which includes the active sites of both ribosomal subunits, together with a bridging element, incorporates less than 6% of the current prokaryotic rRNA, yet it integrates all of the ribosomal components that are vital for synthesizing the earliest coded polypeptides.
Introducing Newborn Screening for Severe Combined Immunodeficiency (SCID) in the Dutch Neonatal Screening Program
Authors: Blom Maartje , Bredius Robbert , Weijman Gert , Dekkers Eugènie , Kemper Evelien , Van den Akker-van Marle M. , Van der Ploeg Catharina , Van der Burg Mirjam , Schielen Peter
Published: December 2018

The implementation of newborn screening for severe combined immunodeficiency (SCID) in the Netherlands is a multifaceted process in which several parties are involved. The Dutch Ministry of Health adopted the advice of the Dutch Health Council to include SCID in the Dutch newborn screening program in 2015. As newborn screening for SCID is executed with a new, relatively expensive assay for the Dutch screening laboratory, an implementation pilot study is deemed instrumental for successful implementation. A feasibility study was performed in which the practicalities and preconditions of expanding the newborn screening program were defined. Cost-effectiveness analysis (CEA) indicated that SCID screening in the Netherlands might be cost-effective, recognizing that there are still many uncertainties in the variables underlying the CEA. Data and experience of the pilot study should provide better estimates of these parameters, thus enabling the actualization of CEA results. Prior to the implementation pilot study, a comparison study of two commercially available SCID screening assays was performed. A prospective implementation pilot study or so-called SONNET study (SCID screening research in the Netherlands with TRECs) started in April 2018 and allows the screening for SCID of all newborns in three provinces of the Netherlands for one year. Based on the results of the SONNET study, the Dutch Ministry of Health will make a final decision about national implementation of newborn screening for SCID in the Netherlands.
An Empirical Study of Exhaustive Matching for Improving Motion Field Estimation
Authors: Lazcano Vanel
Published: December 2018

Optical flow is defined as the motion field of pixels between two consecutive images. Traditionally, in order to estimate pixel motion field (or optical flow), an energy model is proposed. This energy model is composed of (i) a data term and (ii) a regularization term. The data term is an optical flow error estimation and the regularization term imposes spatial smoothness. Traditional variational models use a linearization in the data term. This linearized version of data term fails when the displacement of the object is larger than its own size. Recently, the precision of the optical flow method has been increased due to the use of additional information, obtained from correspondences computed between two images obtained by different methods such as SIFT, deep-matching, and exhaustive search. This work presents an empirical study in order to evaluate different strategies for locating exhaustive correspondences improving flow estimation. We considered a different location for matching random locations, uniform locations, and locations on maximum gradient magnitude. Additionally, we tested the combination of large and medium gradients with uniform locations. We evaluated our methodology in the MPI-Sintel database, which represents the state-of-the-art evaluation databases. Our results in MPI-Sintel show that our proposal outperforms classical methods such as Horn-Schunk, TV-L1, and LDOF, and our method performs similar to MDP-Flow.
A Mobile Acquisition System and a Method for Hips Sway Fluency Assessment
Authors: Civita Andrea , Fiori Simone , Romani Giuseppe
Published: December 2018

The present contribution focuses on the estimation of the Cartesian kinematic jerk of the hips’ orientation during a full three-dimensional movement in the context of enabling eHealth applications of advanced mathematical signal analysis. The kinematic jerk index is estimated on the basis of gyroscopic signals acquired offline through a smartphone. A specific free mobile application is used to acquire the gyroscopic signals and to transmit them to a personal computer through a wireless network. The personal computer elaborates the acquired data and returns the kinematic jerk index associated with a motor task. A comparison of the kinematic jerk index value on a number of data sets confirms that such index can be used to evaluate the fluency of hips orientation during motion. The present research confirms that the proposed gyroscopic data acquisition/processing setup constitutes an inexpensive and portable solution to motion fluency analysis. The proposed data-acquisition and data-processing setup may serve as a supporting eHealth technology in clinical bio-mechanics as well as in sports science.
Cryptic Diversity in Colombian Edible Leaf-Cutting Ants (Hymenoptera: Formicidae)
Authors: Kooij Pepijn W. , Dentinger Bryn M. , Donoso David A. , Shik Jonathan Z. , Gaya Ester
Published: December 2018

Leaf-cutting ants are often considered agricultural pests, but they can also benefit local people and serve important roles in ecosystems. Throughout their distribution, winged reproductive queens of leaf-cutting ants in the genus Atta Fabricius, 1804 are consumed as a protein-rich food source and sometimes used for medical purposes. Little is known, however, about the species identity of collected ants and the accuracy of identification when ants are sold, ambiguities that may impact the conservation status of Atta species as well as the nutritional value that they provide to consumers. Here, 21 samples of fried ants bought in San Gil, Colombia, were identified to species level using Cytochrome Oxidase I (COI) barcoding sequences. DNA was extracted from these fried samples using standard Chelex extraction methods, followed by phylogenetic analyses with an additional 52 new sequences from wild ant colonies collected in Panama and 251 publicly available sequences. Most analysed samples corresponded to Atta laevigata (Smith, 1858), even though one sample was identified as Atta colombica Guérin-Méneville, 1844 and another one formed a distinct branch on its own, more closely related to Atta texana (Buckley, 1860) and Atta mexicana (Smith, 1858). Analyses further confirm paraphyly within Atta sexdens (Linnaeus, 1758) and A. laevigata clades. Further research is needed to assess the nutritional value of the different species.
Comparison of Clinical Manifestations, Antimicrobial Susceptibility Patterns, and Mutations of Fluoroquinolone Target Genes between Elizabethkingia meningoseptica and Elizabethkingia anophelis Isolated in Taiwan
Authors: Lin Jiun-Nong , Lai Chung-Hsu , Yang Chih-Hui , Huang Yi-Han
Published: December 2018

Elizabethkingia meningoseptica and Elizabethkingia anophelis are two major pathogens in the genus Elizabethkingia. Studies have revealed that Elizabethkingia anophelis is frequently misidentified as E. meningoseptica. Therefore, our aim was to explore the clinical and molecular differences between these two species. The database of a clinical microbiology laboratory in a university-affiliated hospital of Taiwan was searched to identify patients with Elizabethkingia infections between January 2005 and June 2018. Species were reidentified using 16S ribosomal RNA gene sequencing. Twenty E. meningoseptica and 72 E. anophelis samples were collected from consecutive patients. E. meningoseptica was significantly more frequently isolated from the cerebrospinal fluid than was E. anophelis. The most susceptible antibiotic for all Elizabethkingia isolates was minocycline (91.3%), followed by levofloxacin (52.2%), tigecycline (23.9%), and piperacillin tazobactam (23.9%). Compared with E. anophelis, E. meningoseptica was significantly less susceptible to piperacillin tazobactam, minocycline, and levofloxacin. Regarding nonsynonymous substitutions in the quinolone-resistance determining regions of DNA gyrase, six sites were recognized in E. meningoseptica and one site was recognized in E. anophelis. E. meningoseptica had a significantly higher rate of fluoroquinolone target gene mutations than did E. anophelis. Because of less susceptibility to multiple antibiotics than E. anophelis, empirical antimicrobial therapy of E. meningoseptica should be more rigorous.
Utility of Novel Cardiorenal Biomarkers in the Prediction and Early Detection of Congestive Kidney Injury Following Cardiac Surgery
Authors: Zelt Jason G. E. , Mielniczuk Lisa M. , Liu Peter P. , Dupuis Jean-Yves , Chih Sharon , Akbari Ayub , Sun Louise Y.
Published: December 2018

Acute Kidney Injury (AKI) in the context of right ventricular failure (RVF) is thought to be largely congestive in nature. This study assessed the utility of biomarkers high sensitivity cardiac troponin T (hs-cTnT), N-Terminal Pro-B-Type Natriuretic Peptide (NT-proBNP), and neutrophil gelatinase-associated lipocalin (NGAL) for prediction and early detection of congestive AKI (c-AKI) following cardiac surgery. This prospective nested case-control study recruited 350 consecutive patients undergoing elective cardiac surgery requiring cardiopulmonary bypass. Cases were patients who developed (1) AKI (2) new or worsening RVF, or (3) c-AKI. Controls were patients free of these complications. Biomarker levels were measured at baseline after anesthesia induction and immediately postoperatively. Patients with c-AKI had increased mean duration of mechanical ventilation and length of stay in hospital and in the intensive care unit (p < 0.01). For prediction of c-AKI, baseline NT-proBNP yielded an area under the curve (AUC) of 0.74 (95% CI, 0.60–0.89). For early detection of c-AKI, postoperative NT-proBNP yielded an AUC of 0.78 (0.66–0.91), postoperative hs-cTnT yielded an AUC of 0.75 (0.58–0.92), and ∆hs-cTnT yielded an AUC of 0.80 (0.64–0.96). The addition of baseline creatinine to ∆hs-cTnT improved the AUC to 0.87 (0.76–0.99), and addition of diabetes improved the AUC to 0.93 (0.88–0.99). Δhs-cTnT alone, or in combination with baseline creatinine or diabetes, detects c-AKI with high accuracy following cardiac surgery.
Finite Element Modelling and Validation of Thermomechanical Behaviour for Layered Aluminium Parts Made by Composite Metal Foil Manufacturing
Authors: Butt Javaid , Ghorabian Mohammad , Ahmed Abed , Shirvani Hassan
Published: December 2018

The paper presents finite element modelling and thermomechanical analysis on the tensile properties of layered aluminium 1050 metal foil parts made by composite metal foil manufacturing. In this paper, a three-dimensional finite element model was developed and validated through experiments to analyse thermal effects on the tensile properties of 200-μm-thick aluminium 1050 metal foils. The effects of thermal stress and strain were studied by carrying out transient thermal analysis on the heated plates used to join the 200-μm-thick metal foils together using a special brazing paste. A standard tensile test at ambient temperature was carried out on the resulting layered dog bone specimens to analyse the thermal effects on the individual layers of metal. The investigations were precisely designed to assess the effect of heat provided amid the brazing operation to join the metal thwarts together as a layered structure and whether it assumed a part in affecting the tensile properties of the final products when contrasted to a solid aluminium 1050 dog bone specimen of the same dimensions. Corrosion testing was also carried out on dog bone specimens made from varying thickness foils (50 μm, 100 μm, and 200 μm) of aluminium 1050 to assess the effect of corrosion on the tensile strength and elongation. The results showed that the specimens did not face the problem of galvanic corrosion of the foil–bond interface. Microstructural analysis was also carried out to analyse the fracture modes of the tested specimens after corrosion testing.
Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma
Authors: Rizo-Gorrita María , Luna-Oliva Irene , Serrera-Figallo María-Angeles , Torres-Lagares Daniel
Published: December 2018

(1) Background. Titanium is characterized by its biocompatibility, resistance to maximum stress, and fatigue and non-toxicity. The composition, surface structure, and roughness of titanium have a key and direct influence on the osseointegration processes when it is used in the form of dental implants. The objective of the present study is to characterize, at chemical, superficial, and biological levels, the result of the application of the sandblasted with large-grit and acid-etched (SLA) treatment consisting of coarse-grained and double-passivated acid blasting with subsequent decontamination with argon plasma on the surface of titanium implants type IV. (2) Methods. Four Oxtein® dental implants (Zaragoza, Spain) were investigated with the following coding: Code L63713T (titanium grade IV, 3.75 mm in diameter, and 13 mm in length). The surface of the implants was SLA type obtained from coarse-grained, double passivated acid, and decontaminated with argon plasma. The samples were in their sealed packages and were opened in our laboratory. The X-ray photoelectron spectroscopy (XPS) technique was used to characterize the chemical composition of the surface, and the scanning electronic microscope (SEM) technique was used to perform topographic surface evaluation. Cell cultures were also performed on both surfaces. (3) Results. The superficial chemical analysis of the studied samples presented the following components, approximately, expressed in atomic percentage: O: 39%; Ti: 18%; C: 39%; N: 2%; and Si: 1%. In the same way, the topographic analysis values were obtained in the evaluated roughness parameters: Ra: 1.5 μm ± 0.02%; Rq: 1.31 μm ± 0.33; Rz: 8.98 μm ± 0.73; Rp: 5.12 μm ± 0.48; Rv: 3.76 μm ± 0.51; and Rc: 4.92 μm ± 0.24. At a biological level, the expression of osteocalcin was higher (p < 0.05) on the micro-rough surface compared to that machined at 48 and 96 h of culture. (4) Conclusions. The data obtained in our study indicate that the total carbon content, the relative concentration of titanium, and the roughness of the treatment performed on the implants are in agreement with those found in the literature. Further, the roughness of the treatment performed on the implants throws a spongy, three-dimensional surface suitable for bone growth on it. The biological results found are compatible with the clinical use of the surface tested.
Image Reconstruction with Reliability Assessment in Quantitative Photoacoustic Tomography
Authors: Hänninen Niko , Pulkkinen Aki , Tarvainen Tanja
Published: December 2018

Quantitative photoacoustic tomography is a novel imaging method which aims to reconstruct optical parameters of an imaged target based on initial pressure distribution, which can be obtained from ultrasound measurements. In this paper, a method for reconstructing the optical parameters in a Bayesian framework is presented. In addition, evaluating the credibility of the estimates is studied. Furthermore, a Bayesian approximation error method is utilized to compensate the modeling errors caused by coarse discretization of the forward model. The reconstruction method and the reliability of the credibility estimates are investigated with two-dimensional numerical simulations. The results suggest that the Bayesian approach can be used to obtain accurate estimates of the optical parameters and the credibility estimates of these parameters. Furthermore, the Bayesian approximation error method can be used to compensate for the modeling errors caused by a coarse discretization, which can be used to reduce the computational costs of the reconstruction procedure. In addition, taking the modeling errors into account can increase the reliability of the credibility estimates.
Experimental Evaluation of Brake Pad Material Propensity to Stick-Slip and Groan Noise Emission
Authors: Lazzari Alessandro , Tonazzi Davide , Conidi Giovanni , Malmassari Cristian , Cerutti Andrea , Massi Francesco
Published: December 2018

Frictional and dynamic responses of brake pad materials, when sliding on brake disc counterfaces, are at the origin of noise, vibration and harshness (NVH) issues such as brake noise emissions. In more detail, groan is a low frequency noise emission often associated to the stick-slip frictional response of the brake system. The instability of such contact is the result of the coupling between the system dynamics and the frictional response of the materials in contact. In this work, an experimental approach is proposed for measuring the frictional response and the propensity to generate stick-slip of different lining materials, coming from commercial brake pads, when sliding on a worn surface of a brake disc, under the same controlled boundary conditions. The proposed methodology allowed for comparing the propensity of the tested pad materials to stick-slip vibrations, which is in agreement with feedback from automotive industry on groan emission.
Synthesis, Characterization and Magnetic Studies of a Tetranuclear Manganese(II/IV) Compound Incorporating an Amino-Alcohol Derived Schiff Base
Authors: Nandy Madhusudan , Shit Shyamapada , Rosair Georgina , Gómez-García Carlos J.
Published: December 2018

A new tetranuclear mixed-valence manganese(II/IV) compound [MnIIMnIV3(μ-Cl)3(µ3-O)(L)3] (1) (where H3L = (3E)-3-((Z)-4-hydroxy-4-phenylbut-3-en-2-ylideneamino)propane-1,2-diol) has been synthesized and characterized by different physicochemical methods. Single crystal X-ray diffraction analysis reveals that 1 is a tetrahedral cluster consisting of a Mn4Cl3O4 core in which the only Mn(II) ion is joined through three μ2-O bridges to an equilateral triangle of Mn(IV) ions, which are connected by a μ3-O and three μ2-Cl bridges. The redox behavior of 1 was studied by cyclic voltammetry. Variable temperature magnetic susceptibility measurements of 1 revealed predominant antiferromagnetic coupling inside the Mn4Cl3O4 cluster.
Classification Theorems of Ruled Surfaces in Minkowski Three-Space
Authors: Choi Miekyung , Kim Young Ho
Published: December 2018

By generalizing the notion of the pointwise 1-type Gauss map, the generalized 1-type Gauss map has been recently introduced. Without any assumption, we classified all possible ruled surfaces with the generalized 1-type Gauss map in a 3-dimensional Minkowski space. In particular, null scrolls do not have the proper generalized 1-type Gauss map. In fact, it is harmonic.
Double Controlled Metric Type Spaces and Some Fixed Point Results
Authors: Abdeljawad Thabet , Mlaiki Nabil , Aydi Hassen , Souayah Nizar
Published: December 2018

In this article, in the sequel of extending b-metric spaces, we modify controlled metric type spaces via two control functions α ( x , y ) and μ ( x , y ) on the right-hand side of the b - triangle inequality, that is, d ( x , y ) ≤ α ( x , z ) d ( x , z ) + μ ( z , y ) d ( z , y ) , for all x , y , z ∈ X . Some examples of a double controlled metric type space by two incomparable functions, which is not a controlled metric type by one of the given functions, are presented. We also provide some fixed point results involving Banach type, Kannan type and ϕ -nonlinear type contractions in the setting of double controlled metric type spaces.
The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme
Authors: Andreo-Vidal Andrés , Sanchez-Amat Antonio , Campillo-Brocal Jonatan C.
Published: December 2018

The marine environment is a rich source of antimicrobial compounds with promising pharmaceutical and biotechnological applications. The Pseudoalteromonas genus harbors one of the highest proportions of bacterial species producing antimicrobial molecules. For decades, the presence of proteins with L-amino acid oxidase (LAAO) and antimicrobial activity in Pseudoalteromonas luteoviolacea has been known. Here, we present for the first time the identification, cloning, characterization and phylogenetic analysis of Pl-LAAO, the enzyme responsible for both LAAO and antimicrobial activity in P. luteoviolacea strain CPMOR-2. Pl-LAAO is a flavoprotein of a broad substrate range, in which the hydrogen peroxide generated in the LAAO reaction is responsible for the antimicrobial activity. So far, no protein with a sequence similarity to Pl-LAAO has been cloned or characterized, with this being the first report on a flavin adenine dinucleotide (FAD)-containing LAAO with antimicrobial activity from a marine microorganism. Our results revealed that 20.4% of the sequenced Pseudoalteromonas strains (specifically, 66.6% of P. luteoviolacea strains) contain Pl-laao similar genes, which constitutes a well-defined phylogenetic group. In summary, this work provides insights into the biological significance of antimicrobial LAAOs in the Pseudoalteromonas genus and shows an effective approach for the detection of novel LAAOs, whose study may be useful for biotechnological applications.
Effect of Cu on the Fracture and Exfoliation Corrosion Behavior of Al-Zn-Mg-xCu Alloy
Authors: Jiao Huibin , Chen Kanghua , Chen Songyi , Yang Zhen , Xie Peng , Chen Shanda
Published: December 2018

In the present work, the influence of Cu content on microstructure, mechanical properties and exfoliation corrosion behaviors of Al-Zn-Mg-xCu alloy extrusions has been investigated in longitudinal-transverse (L-T) and short-longitudinal (S-L) directions by means of mechanical tensile and exfoliation corrosion testing combined with optical microscopy (OM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results showed that a higher Cu content significantly decreased the fracture toughness and ductility of the alloy in S-L direction compared with L-T direction. Concomitant with the increase in Cu content, a transition in fracture mode was observed from transgranular dimpled rupture to intergranular rupture in S-L direction. Moreover, the exfoliation corrosion (EXCO) resistance of the alloy decreased as the Cu content increased and the exfoliation corrosion resistance of the alloy in short-transverse (S-T) direction was better than that of L-T direction. These results were mainly associated with the large number of coarse intermetallics caused by high Cu content in the L-T direction of alloy.
Numerical and Experimental Study on the Thermodynamic Coupling of Ti-6Al-4V Blade Preforms by Cross Wedge Rolling
Authors: Ji Hongchao , Dong Jianwei , Xin Long , Huang Xiaomin , Liu Jinping
Published: December 2018

Titanium alloy possesses high strength, good corrosion resistance, and high heat resistance; thus, it is widely used in the aerospace and other fields. Blades of titanium alloy are important components of aero-engines and are essential to the engines operation. In this work, a Ti-6Al-4V blade was formed by cross wedge rolling (CWR) to realize the near net-shape of an aero-engine blade. First, thermal simulation experiments of Ti-6Al-4V were carried out to obtain the thermal deformation constitutive equation of the alloy. The finite element software Deform-3D was then used to simulate the thermodynamic coupling of the forming process, and the metal flow, temperature, and stress–strain distribution laws during the forming process were analyzed. Finally, experimental verification of the Ti-6Al-4V blade was carried out by using an H500 CWR mill. The results revealed the feasibility of applying CWR to preform Ti-6Al-4V blades.
White-Light Photosensors Based on Ag Nanoparticle-Reduced Graphene Oxide Hybrid Materials
Authors: Tu Wei-Chen , Liu Xiang-Sheng , Chen Shih-Lun , Lin Ming-Yi , Uen Wu-Yih , Chen Yu-Cheng , Chao Yu-Chiang
Published: December 2018

The unique and outstanding electrical and optical properties of graphene make it a potential material to be used in the construction of high-performance photosensors. However, the fabrication process of a graphene photosensor is usually complicated and the size of the device also is restricted to micrometer scale. In this work, we report large-area photosensors based on reduced graphene oxide (rGO) implemented with Ag nanoparticles (AgNPs) via a simple and cost-effective method. To further optimize the performance of photosensors, the absorbance and distribution of the electrical field intensity of graphene with AgNPs was simulated using the finite-difference time-domain (FDTD) method through use of the surface plasmon resonance effect. Based on the simulated results, we constructed photosensors using rGO with 60–80 nm AgNPs and analyzed the characteristics at room temperature under white-light illumination for outdoor environment applications. The on/off ratio of the photosensor with AgNPs was improved from 1.166 to 9.699 at the bias voltage of −1.5 V, which was compared as a sample without AgNPs. The proposed photosensor affords a new strategy to construct cost-effective and large-area graphene films which raises opportunities in the field of next-generation optoelectronic devices operated in an outdoor environment.
Membrane and Cytoplasmic Proteins of Mycobacterium avium subspecies paratuberculosis that Bind to Novel Monoclonal Antibodies
Authors: Bannantine John P. , Stabel Judith R. , Lippolis John D. , Reinhardt Timothy A.
Published: December 2018

Monoclonal antibodies against Mycobacterium avium subspecies paratuberculosis (Map) proteins are important tools in Johne’s disease research and diagnostics. Johne’s disease is a chronic inflammatory intestinal disease of cattle, sheep, and other ruminant animals. We have previously generated multiple sets of monoclonal antibodies (mAbs) in different studies; however, because many were generated and screened against a whole-cell extract of Map, the antigens that bind to these antibodies remained unknown. In this study, we used three different approaches to identify the corresponding Map antigens for 14 mAbs that could not be identified previously. In the first approach, a new Map-lambda phage expression library was screened to identify corresponding antigens for 11 mAbs. This approach revealed that mAbs 7C8, 9H3, 12E4, 3G5, and 11B8 all detect MAP_3404 encoding the biotin carboxylase subunit of acetyl-CoA carboxylase, while mAbs 7A6, 11F8, and 10C12 detect the GroEL2 chaperonin (MAP_3936), 6C9 detects electron transfer flavoprotein (MAP_3060c), and 14G11 detects MAP_3976, a lipoprotein anchoring transpeptidase. The epitopes to a selection of these mAbs were also defined. In a second approach, MAP_2698c bound monoclonal antibody (mAb) 14D4 as determined using protein arrays. When both of these approaches failed to identify the antigen for mAb 12C9, immunoprecipitation, mass spectrometry analysis, and codon optimization was used to identify the membrane protein, MAP_4145, as the reacting antigen. Characterized antibodies were used to quickly interrogate mycobacterial proteomic preps. We conclude by providing a complete catalog of available mAbs to Map proteins, along with their cognate antigens and epitopes, if known. These antibodies are now thoroughly characterized and more useful for research and diagnostic purposes.
Occurrence of Felsic Rocks in Oceanic Gabbros from IODP Hole U1473A: Implications for Evolved Melt Migration in the Lower Oceanic Crust
Authors: Nguyen Du Khac , Morishita Tomoaki , Soda Yusuke , Tamura Akihiro , Ghosh Biswajit , Harigane Yumiko , France Lydéric , Liu Chuanzhou , Natland James H. , Sanfilippo Alessio , MacLeod Christopher J. , Blum Peter , Dick Henry J. B.
Published: December 2018

Felsic rocks are minor in abundance but occur ubiquitously in International Ocean Discovery Program Hole U1473A, Southwest Indian Ridge. The trace element abundances of high-Ti brown amphibole, plagioclase, and zircon in veins, as well as the presence of myrmekitic texture in the studied felsic rocks support crystallization origin from highly-evolved melts, probably controlled by fractional crystallization. Based on geochemical criteria and texture of the mineral assemblage in felsic rocks and their relationship with host gabbros, they can be divided into three types: (1) Felsic rock with sharp boundaries is formed when felsic melt intrudes into fractures of host gabbros, resulting in minimal interaction between the melt and the wall minerals. (2) Replacive felsic rock, which is characterized by a pseudomorphic replacement of minerals in the host gabbro. This vein type is caused by the replacement of the host mineralogy by minerals in equilibrium with the felsic melts. (3) Felsic rock with diffused boundaries is formed either by infiltration of felsic melt into the solidifying gabbro body or crystallization of interstitial melts. Infiltration modes of felsic melts are likely controlled by the temperature condition of the cooling host gabbros.
Simultaneous Quantitation of Isoprenoid Pyrophosphates in Plasma and Cancer Cells Using LC-MS/MS
Authors: Chhonker Yashpal S. , Haney Staci L. , Bala Veenu , Holstein Sarah A. , Murry Daryl J.
Published: December 2018

Isoprenoids (IsoP) are an important class of molecules involved in many different cellular processes including cholesterol synthesis. We have developed a sensitive and specific LC-MS/MS method for the quantitation of three key IsoPs in bio-matrices, geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP). LC-MS/MS analysis was performed using a Nexera UPLC System connected to a LCMS-8060 (Shimadzu Scientific Instruments, Columbia, MD) with a dual ion source. The electrospray ionization source was operated in the negative MRM mode. The chromatographic separation and detection of analytes was achieved on a reversed phase ACCQ-TAG Ultra C18 (1.7 µm, 100 mm × 2.1 mm I.D.) column. The mobile phase consisted of (1) a 10 mM ammonium carbonate with 0.1% ammonium hydroxide in water, and (2) a 0.1% ammonium hydroxide in acetonitrile/methanol (75/25). The flow rate was set to 0.25 mL/min in a gradient condition. The limit of quantification was 0.04 ng/mL for all analytes with a correlation coefficient (r2) of 0.998 or better and a total run time of 12 min. The inter- and intra-day accuracy (85–115%) precision (<15%), and recovery (40–90%) values met the acceptance criteria. The validated method was successfully applied to quantitate basal concentrations of GPP, FPP and GGPP in human plasma and in cultured cancer cell lines. Our LC-MS/MS method may be used for IsoP quantification in different bio-fluids and to further investigate the role of these compounds in various physiological processes.
Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells
Authors: Liang Chuxin , Chen Chang , Zhou Pengfei , Xu Lv , Zhu Jianhua , Liang Jincai , Zi Jiachen , Yu Rongmin
Published: December 2018

This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism. According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus CMCs for 6 day, 25 mg/L A. flavus mycelium elicitor were added, and the CMC suspensions were further cultured for another 48 h. In this condition, the contents of vindoline, catharanthine, and ajmaline were 1.45-, 3.29-, and 2.14-times as high as those of the control group, respectively. Transcriptome analysis showed that D4H, G10H, GES, IRS, LAMT, SGD, STR, TDC, and ORCA3 were involved in the regulation of this induction process. The results of qRT-PCR indicated that the increasing accumulations of vindoline, catharanthine, and ajmaline in C. roseus CMCs were correlated with the increasing expression of the above genes. Therefore, A. flavus fungal elicitor could enhance the TIA production of C. roseus CMCs, which might be used as an alternative biotechnological resource for obtaining bioactive alkaloids.