bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNA-Based Fingerprinting of Cervical Lesions and Cancer
Authors: Pisarska Justyna , Baldy-Chudzik Katarzyna
Published: November 2020

The regulatory functions of microRNA (miRNA) are involved in all processes contributing to carcinogenesis and response to viral infections. Cervical cancer in most cases is caused by the persistence of high-risk human papillomavirus (HR-HPV) infection. While oncogenic human papillomaviruses induce aberrant expression of many cellular miRNAs, this dysregulation could be harnessed as a marker in early diagnosis of HR-HPV infection, cervical squamous intraepithelial lesions, and cancer. In recent years, growing data indicate that miRNAs show specific patterns at various stages of cervical pathology. The aim of this review is to systematize current reports on miRNA capacity that can be utilized in personalized diagnostics of cervical precancerous and cancerous lesions. The analysis of the resources available in online databases (National Center for Biotechnology Information—NCBI, PubMed, ScienceDirect, Scopus) was performed. To date, no standardized diagnostic algorithm using the miRNA pattern in cervical pathology has been defined. However, the high sensitivity and specificity of the reported assays gives hope for the development of non-invasive diagnostic tests that take into account the heterogeneity of tumor-related changes. Due to this variability resulting in difficult to predict clinical outcomes, precise molecular tools are needed to improve the diagnostic and therapeutic process.
What Type of Patients Did PARAGON-HF Select? Insights from a Real-World Prospective Cohort of Patients with Heart Failure and Preserved Ejection Fraction
Authors: Rettl René , Dachs Theresa-Marie , Duca Franz , Binder Christina , Dusik Fabian , Seirer Benjamin , Schönauer Johannes , Kronberger Christina , Camuz Ligios Luciana , Hengstenberg Christian , Derkits Nina , Kastner Johannes , Badr Eslam Roza , Bonderman Diana
Published: November 2020

The PARAGON-HF clinical trial suggested that sacubitril/valsartan may become a treatment option for particular subgroups of patients with heart failure and preserved ejection fraction (HFpEF). However, the proportion of real-world HFpEF patients who are theoretically superimposable with the PARAGON-HF population is yet unknown. The present study was performed to define the proportion of real-world PARAGON-HF-like patients and to describe their clinical characteristics and long-term prognosis in comparison with those who would not meet PARAGON-HF criteria. We systematically applied PARAGON-HF inclusion and exclusion criteria to a total of 427 HFpEF patients who have been participating in a prospective national registry between December 2010 and December 2019. In total, only 170 (39.8%) registry patients were theoretically eligible for PARAGON-HF. Patients not meeting inclusion criteria (41.0%) were less impaired with respect to exercise capacity (median 6-min walk distance: 385 m (IQR: 300–450) versus 323 m (IQR: 240–383); p < 0.001) had lower pulmonary pressures (mean pulmonary artery pressure (mPAP): 31.2 mmHg, standard deviation (SD): ±10.2 versus 32.8 mmHg, SD: ±9.7; p < 0.001) and better outcomes (log-rank: p < 0.001) as compared to the PARAGON-like cohort. However, patients theoretically excluded from the trial (19.2%) were those with most advanced heart failure symptoms (median 6-min walk test: 252 m (IQR: 165–387); p < 0.001), highest pulmonary pressures (mPAP: 38.2 mmHg, SD: ±12.4; p < 0.001) and worst outcome (log-rank: p = 0.037). We demonstrate here that < 40% of real-world HFpEF patients meet eligibility criteria for PARAGON-HF. We conclude that despite reasons for optimism after PARAGON-HF, a large proportion of HFpEF patients will remain without meaningful treatment options.
Fluid Shifts Induced by Physical Therapy in Lower Limb Lymphedema Patients
Authors: Brix Bianca , Apich Gert , Roessler Andreas , Ure Christian , Schmid-Zalaudek Karin , Hinghofer-Szalkay Helmut , Goswami Nandu
Published: November 2020

Complete decongestive therapy (CDT), a physical therapy including manual lymphatic drainage (MLD) and compression bandaging, is aimed at mobilizing fluid and reducing limb volume in lymphedema patients. Details of fluid shifts occurring in response to CDT are currently not well studied. Therefore, we investigated fluid shifts before, during and after CDT. Thirteen patients (3 males and 10 females, aged 57 ± 8.0 years, 167.2 ± 8.3 cm height, 91.0 ± 23.4 kg weight) diagnosed with stage II leg lymphedema participated. Leg volume, limb and whole-body fluid composition (total body water (limbTBW/%TBW), extracellular (limbECF/%ECF) and intracellular (limbICF/%ICF fluid), as well as ECF/ICF and limbECF/limbICF ratios were determined using perometry and bioelectrical impedance spectroscopy. Plasma volume, proteins, osmolality, oncotic pressure and electrolytes were assessed. Leg volume (p < 0.001), limbECF (p = 0.041), limbICF (p = 0.005) and limbECF/limbICF decreased over CDT. Total leg volume and limbTBW were correlated (r = 0.635). %TBW (p = 0.001) and %ECF (p = 0.007) decreased over time. The maximum effects were seen within one week of CDT. LimbICF (p = 0.017), %TBW (p = 0.009) and %ICF (p = 0.003) increased post-MLD, whereas ECF/ICF decreased due to MLD. Plasma volume increased by 1.5% post-MLD, as well as albumin and the albumin-to-globulin ratio (p = 0.005 and p = 0.049, respectively). Our results indicate that physical therapy leads to fluid shifts in lymphedema patients, with the greatest effects occurring within one week of therapy. Fluid shifts due to physical therapy were also reflected in increased plasma volume and plasma protein concentrations. Perometry, in contrast to bioelectrical impedance analysis, does not seem to be sensitive enough to detect small fluid changes caused by manual lymphatic drainage.
A Retrospective Analysis about Frequency of Monitoring in Italian Chronic Myeloid Leukemia Patients after Discontinuation
Authors: Dragani Matteo , Rege Cambrin Giovanna , Berchialla Paola , Dogliotti Irene , Rosti Gianantonio , Castagnetti Fausto , Capodanno Isabella , Martino Bruno , Cerrano Marco , Ferrero Dario , Gambacorti-Passerini Carlo , Crugnola Monica , Elena Chiara , Breccia Massimo , Iurlo Alessandra , Cattaneo Daniele , Galimberti Sara , Gozzini Antonella , Bocchia Monica , Lunghi Francesca , Cedrone Michele , Sgherza Nicola , Luciano Luigia , Russo Sabina , Santoro Marco , Giai Valentina , Caocci Giovanni , Levato Luciano , Abruzzese Elisabetta , Sora Federica , Saglio Giuseppe , Fava Carmen
Published: November 2020

Successful discontinuation of tyrosine kinase inhibitors has been achieved in patients with chronic-phase chronic myeloid leukemia (CML). Careful molecular monitoring after discontinuation warrants safe and prompt resumption of therapy. We retrospectively evaluated how molecular monitoring has been conducted in Italy in a cohort of patients who discontinued tyrosine kinase inhibitor (TKI) treatment per clinical practice. The outcome of these patients has recently been reported—281 chronic-phase CML patients were included in this subanalysis. Median follow-up since discontinuation was 2 years. Overall, 2203 analyses were performed, 17.9% in the first three months and 38.4% in the first six months. Eighty-six patients lost major molecular response (MMR) in a mean time of 5.7 months—65 pts (75.6%) during the first six months. We evaluated the number of patients who would experience a delay in diagnosis of MMR loss if a three-month monitoring schedule was adopted. In the first 6 months, 19 pts (29.2%) would have a one-month delay, 26 (40%) a 2-month delay. Very few patients would experience a delay in the following months. A less intense frequency of monitoring, particularly after the first 6 months off treatment, would not have affected the success of treatment-free remission (TFR) nor put patients at risk of progression.
Toward a Highly Accurate Classification of Underwater Cable Images via Deep Convolutional Neural Network
Authors: Thum Guan Wei , Tang Sai Hong , Ahmad Siti Azfanizam , Alrifaey Moath
Published: November 2020

Underwater cables or pipelines are commonly utilized elements in ocean research, marine engineering, power transmission, and communication-based activities. Their performance necessitates regularly conducted inspection for maintenance purposes. A vision system is commonly used by autonomous underwater vehicles (AUVs) to track and search for underwater cable. Its traditional methods are characteristically applicable in AUVs, wherein they are equipped with handcrafted features and shallow trainable architectures. However, such methods are subpar or even incapable of tracking underwater cable in fast-changing and complex underwater conditions. In contrast to this, the deep learning method is linked with the capacity to learn semantic, high-level, and deeper features, thus rendering it recommended for performing underwater cable tracking. In this study, several deep Convolutional Neural Network (CNN) models were proposed to classify underwater cable images obtained from a set of underwater images, whereby transfer learning and data augmentation were applied to enhance the classification accuracy. Following a comparison and discussion regarding the performance of these models, MobileNetV2 outperformed among other models and yielded lower computational time and the highest accuracy for classifying underwater cable images at 93.5%. Hence, the main contribution of this study is geared toward developing a deep learning method for underwater cable image classification.
Environmental Screening of Fonsecaea Agents of Chromoblastomycosis Using Rolling Circle Amplification
Authors: Voidaleski Morgana Ferreira , Gomes Renata Rodrigues , Azevedo Conceição de Maria Pedrozo e Silva de , Lima Bruna Jacomel Favoreto de Souza , Costa Flávia de Fátima , Bombassaro Amanda , Fornari Gheniffer , Cristina Lopes da Silva Isabelle , Andrade Lucas Vicente , Lustosa Bruno Paulo Rodrigues , Najafzadeh Mohammad J. , de Hoog G. Sybren , Vicente Vânia Aparecida
Published: November 2020

Chromoblastomycosis is a chronic, cutaneous or subcutaneous mycosis characterized by the presence of muriform cells in host tissue. Implantation disease is caused by melanized fungi related to black yeasts, which, in humid tropical climates, are mainly members of the genus Fonsecaea. In endemic areas of Brazil, F. pedrosoi and F. monophora are the prevalent species. The current hypothesis of infection is traumatic introduction via plant materials, especially by plant thorns. However, isolation studies have demonstrated a low frequency of the agents in environmental substrates. The present study aimed to detect F. pedrosoi and F. monophora in shells of babassu coconuts, soil, plant debris, and thorns from endemic areas of chromoblastomycosis in Maranhão state, northern Brazil, using Rolling Circle Amplification (RCA) with padlock probes as a new environmental screening tool for agents of chromoblastomycosis. In addition to molecular screening, the environmental samples were analyzed by fungal isolation using mineral oil flotation. The limit of detection of the RCA method was 2.88 × 107 copies of DNA per sample for the used padlock probes, indicating that this represents an efficient and sensitive molecular tool for the environmental screening of Fonsecaea agents. In contrast, with isolation from the same samples using several selective methods, no agents of chromoblastomycosis were recovered.
Bank Profitability and Efficiency in Portugal and Spain: A Non-Linearity Approach
Authors: Neves Maria Elisabete , Proença Catarina , Dias António
Published: November 2020

This paper aims to analyze the determinants of profitability and bank efficiency in the Iberian Peninsula. To achieve the proposed objective, a sample of 66 Portuguese and Spanish banks was analyzed. To test the hypotheses formulated according to the proposed literature review, the panel data methodology was used; specifically, the Generalized Method of Moments (GMM) system model proposed by and the Tobit model. The results point out that the banking performance, measured in terms of profitability and efficiency, in the Iberian Peninsula, is influenced by internal management variables, but also by the macroeconomic environment. More interestingly, and new in the Iberian banking sector literature, the results prove a positive and negative non-linear relationship between bank size and their levels of profitability and efficiency, respectively.
The Syntactic Distribution of Object Experiencer Psych Verbs in Heritage Spanish
Authors: Gonzalez Becky Halloran
Published: November 2020

This paper contributes to our understanding of the grammatical architecture of heritage languages and, specifically, the role of lexical semantics, by examining the syntactic distribution of Spanish psych verbs. Object experiencer psych verbs in Spanish fall into two classes: Class II (e.g., molestar “to bother”) and Class III (e.g., encantar “to love”). Class II verbs allow numerous syntactic alternations, while Class III verbs are more restricted syntactically. The asymmetry under investigation here is attributed to a lexical semantic featural difference—Class II verbs can be [±change of state], while Class III verbs are always [−change of state]. Two groups of HSs, (intermediate (n = 21) and advanced (n = 18)), and a group of Spanish dominant bilinguals (n = 19) completed two judgment tasks, a standard proficiency measure, a vocabulary task, and a biographical questionnaire. Results reveal that the responses of both HS groups are consistent with the Spanish dominant bilinguals in nearly all conditions, indicating that HSs are highly sensitive to this syntactic distribution. These results also highlight the importance of considering the results of individual verbs in studies that focus on lexical semantics, as they not only help us understand aggregate trends, but also reveal, in this case, that even in cases of deviant underlying semantic representations, licensing restrictions at the syntax-lexical semantic interface remain intact, suggesting that this is an area of resilience in the Heritage Spanish grammar.
Parametric Analysis and Stiffness Investigation of Extended End-Plate Connection
Authors: Luo Liang , Du Maohua , Yuan Jian , Shi Jun , Yu Suhui , Zhang Yi
Published: November 2020

Extended end-plate (EP) bolted connections are widely used in steel structures as moment-resisting connections. Most of these connections are semi-rigid or in other words flexible. The paper aims to study the behavior of such connections under the effect of column top-side cyclic loading using the finite element (FE) method. For semi-rigid connections, it is very vital to determine the moment-rotation relationship as well as the connection stiffness. These beam-column connections have been parametrically studied, the effect of joint type, shear forces, diameter of bolt, thickness of end-plate, and end-plate style were studied. Parametric studies show that the panel zone shear force is the key factor and has a significant effect on the connection stiffness. Finally, based on the component method, the stiffness of the bending component is improved, and the initial stiffness calculation model of the connection under column top-side cyclic loadings is established. The results show that the calculation model is in good agreement with the finite element analyses, and this proves that the calculation model proposed in this study could act as a reference method.
Reverse Mortgage Risks. Time Evolution of VaR in Lump-Sum Solutions
Authors: Fuente Iván de la , Navarro Eliseo , Serna Gregorio
Published: November 2020

In this study, we analyzed the risk faced by the reverse mortgage provider in the case of the lump-sum solution, which is increasingly becoming one of the most popular types of reverse mortgages. The risk faced by the mortgage provider was estimated by means of a value at risk (VaR) procedure that involves a Monte Carlo simulation method and an ARMA-EGARCH assumption for modeling house price returns in the United Kingdom from 1952 to 2019. The results showed that the reverse mortgage provider faced higher risk and consequently needed to allocate more funds to meet its regulatory capital requirements in the case of relatively young borrowers, especially when they reached their life expectancy and had high roll-up rates. The risk was even higher in the case of the female population. Furthermore, care must be taken when the rental yield rate is higher than the risk-free rate, as is currently the case, as the value of the no-negative-equity guarantee (NNEG) is relatively high and results in higher value at risk (VaR) and expected shortfall (ES) values. These results have important implications in terms of policy decision making when determining the countercyclical buffer for reverse mortgages in Basel III, as well as from a managerial perspective when determining the economic capital needed to support the risk taken by the lender.
Eosinophilic Esophagitis as a Side Effect of Food Oral Immunotherapy
Authors: Cianferoni Antonella
Published: November 2020

Food allergies (FAs) include a spectrum of immune-mediated serious and potentially life-threatening medical conditions with an overall estimated prevalence ranging from 4% to 8% in the U.S. and Europe. Significant progress in food allergen-specific immunotherapy has been accomplished over the past 10 years. The most studied strategy has been oral immunotherapy (OIT), also known as food desensitization, a treatment in which a child is slowly and deliberately given a small amount of the food to ingest (that previously was a food allergy trigger) with the ultimate goal of the child eating that food without a reaction. OIT is now recommended in the European guidelines for the treatment of milk, egg, and peanut allergies and was the first American Food Drug Administration (FDA) approved product for the prevention of severe reaction to peanuts in 4–17 year olds to be released on the market. The side effects associated with OIT treatment trials are mild to moderate, predominantly oropharyngeal, and easily treated. More severe reactions, such as generalized urticaria/angioedema, wheezing/respiratory distress, laryngeal edema, and repetitive emesis, have been reported. However systemic reactions are very rare. Low-dose immunotherapy is associated with significantly fewer side effects. Currently, its most limiting allergic side effect is that approximately 10–15% of subjects treated with OIT experience gastrointestinal symptoms, preventing the continuation of therapy. Eosinophilic esophagitis (EoE) has also been reported as a cause of persistent abdominal symptoms in OIT.
Application of Crosslinked Polybenzimidazole-Poly(Vinyl Benzyl Chloride) Anion Exchange Membranes in Direct Ethanol Fuel Cells
Authors: Herranz Daniel , Coppola Roxana E. , Escudero-Cid Ricardo , Ochoa-Romero Kerly , D’Accorso Norma B. , Pérez-Flores Juan Carlos , Canales-Vázquez Jesús , Palacio Carlos , Abuin Graciela C. , Ocón Pilar
Published: November 2020

Crosslinked membranes have been synthesized by a casting process using polybenzimidazole (PBI) and poly(vinyl benzyl chloride) (PVBC). The membranes were quaternized with 1,4-diazabicyclo[2.2.2]octane (DABCO) to obtain fixed positive quaternary ammonium groups. XPS analysis has showed insights into the changes from crosslinked to quaternized membranes, demonstrating that the crosslinking reaction and the incorporation of DABCO have occurred, while the 13C-NMR corroborates the reaction of DABCO with PVBC only by one nitrogen atom. Mechanical properties were evaluated, obtaining maximum stress values around 72 MPa and 40 MPa for crosslinked and quaternized membranes, respectively. Resistance to oxidative media was also satisfactory and the membranes were evaluated in single direct ethanol fuel cell. PBI-c-PVBC/OH 1:2 membrane obtained 66 mW cm−2 peak power density, 25% higher than commercial PBI membranes, using 0.5 bar backpressure of pure O2 in the cathode and 1 mL min−1 KOH 2M EtOH 2 M aqueous solution in the anode. When the pressure was increased, the best performance was obtained by the same membrane, reaching 70 mW cm−2 peak power density at 2 bar O2 backpressure. Based on the characterization and single cell performance, PBI-c-PVBC/OH membranes are considered promising candidates as anion exchange electrolytes for direct ethanol fuel cells.
Separation of Radioactive Elements from Rare Earth Element-Bearing Minerals
Authors: García Adrián Carrillo , Latifi Mohammad , Amini Ahmadreza , Chaouki Jamal
Published: November 2020

Rare earth elements (REE), originally found in various low-grade deposits in the form of different minerals, are associated with gangues that have similar physicochemical properties. However, the production of REE is attractive due to their numerous applications in advanced materials and new technologies. The presence of the radioactive elements, thorium and uranium, in the REE deposits, is a production challenge. Their separation is crucial to gaining a product with minimum radioactivity in the downstream processes, and to mitigate the environmental and safety issues. In the present study, different techniques for separation of the radioactive elements from REE are reviewed, including leaching, precipitation, solvent extraction, and ion chromatography. In addition, the waste management of the separated radioactive elements is discussed with a particular conclusion that such a waste stream can be employed as a valuable co-product.
Editorial for the Special Issue on Miniaturized Silicon Photodetectors: New Perspectives and Applications
Authors: Casalino Maurizio
Published: November 2020

Silicon (Si) technologies provide an excellent platform for monolithically integrating both photonic [...]
Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity
Authors: Liakh Ivan , Sledzinski Tomasz , Kaska Lukasz , Mozolewska Paulina , Mika Adriana
Published: November 2020

Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also used in healthy, nonobese subjects or patients with other dyslipidemias. This review is an overview of sample preparation methods for analysis as one of the major critical steps in the overall analytical procedure.
DNA Intercalating Near-Infrared Luminescent Lanthanide Complexes Containing Dipyrido[3,2-a:2′,3′-c]phenazine (dppz) Ligands: Synthesis, Crystal Structures, Stability, Luminescence Properties and CT-DNA Interaction
Authors: Savić Aleksandar , Kaczmarek Anna M. , Van Deun Rik , Van Hecke Kristof
Published: November 2020

In order to create near-infrared (NIR) luminescent lanthanide complexes suitable for DNA-interaction, novel lanthanide dppz complexes with general formula [Ln(NO3)3(dppz)2] (Ln = Nd3+, Er3+ and Yb3+; dppz = dipyrido[3,2-a:2′,3′-c]phenazine) were synthesized, characterized and their luminescence properties were investigated. In addition, analogous compounds with other lanthanide ions (Ln = Ce3+, Pr3+, Sm3+, Eu3+, Tb3+, Dy3+, Ho3+, Tm3+, Lu3+) were prepared. All complexes were characterized by IR spectroscopy and elemental analysis. Single-crystal X-ray diffraction analysis of the complexes (Ln = La3+, Ce3+, Pr3+, Nd3+, Eu3+, Er3+, Yb3+, Lu3+) showed that the lanthanide’s first coordination sphere can be described as a bicapped dodecahedron, made up of two bidentate dppz ligands and three bidentate-coordinating nitrate anions. Efficient energy transfer was observed from the dppz ligand to the lanthanide ion (Nd3+, Er3+ and Yb3+), while relatively high luminescence lifetimes were detected for these complexes. In their excitation spectra, the maximum of the strong broad band is located at around 385 nm and this wavelength was further used for excitation of the chosen complexes. In their emission spectra, the following characteristic NIR emission peaks were observed: for a) Nd3+: 4F3/2 → 4I9/2 (870.8 nm), 4F3/2 → 4I11/2 (1052.7 nm) and 4F3/2 → 4I13/2 (1334.5 nm); b) Er3+: 4I13/2 → 4I15/2 (1529.0 nm) c) Yb3+: 2F5/2 → 2F7/2 (977.6 nm). While its low triplet energy level is ideally suited for efficient sensitization of Nd3+ and Er3+, the dppz ligand is considered not favorable as a sensitizer for most of the visible emitting lanthanide ions, due to its low-lying triplet level, which is too low for the accepting levels of most visible emitting lanthanides. Furthermore, the DNA intercalation ability of the [Nd(NO3)3(dppz)2] complex with calf thymus DNA (CT-DNA) was confirmed using fluorescence spectroscopy.
Pharmacological Characterization of 4-Methylthioamphetamine Derivatives
Authors: Guajardo Fabrizzio G. , Velásquez Victoria B. , Raby Daniela , Núñez-Vivanco Gabriel , Iturriaga-Vásquez Patricio , España Rodrigo A. , Reyes-Parada Miguel , Sotomayor-Zárate Ramón
Published: November 2020

Amphetamine derivatives have been used in a wide variety of pathologies because of their pharmacological properties as psychostimulants, entactogens, anorectics, and antidepressants. However, adverse cardiovascular effects (sympathomimetics) and substance abuse problems (psychotropic and hallucinogenic effects) have limited their use. 4-Methylthioamphetamine (MTA) is an amphetamine derivative that has shown to inhibit monoamine uptake and monoamine oxidase. However, the pharmacological characterization (neurochemical, behavioral, and safety) of its derivatives 4-ethylthioamphetamine (ETA) and 4-methylthio-phenil-2-butanamine (MT-But) have not been studied. In the current experiments, we show that ETA and MT-But do not increase locomotor activity and conditioned place preference with respect to MTA. At the neurochemical level, ETA and MT-But do not increase in vivo DA release in striatum, but ETA and MT-But affect the nucleus accumbens bioaccumulation of DA and DOPAC. Regarding cardiovascular effects, the administration of MTA and ETA increased the mean arterial pressure and only ETA significantly increases the heart rate. Our results show that the pharmacological and safety profiles of MTA are modulated by changing the methyl-thio group or the methyl group of the aminoethyl chain.
LC-MS Quantification of Site-Specific Phosphorylation Degree by Stable-Isotope Dimethyl Labeling Coupled with Phosphatase Dephosphorylation
Authors: Chen Sin-Hong , Lin Ya-Chi , Shih Ming-Kuei , Wang Li-Fei , Liu Shyh-Shyan , Hsu Jue-Liang
Published: November 2020

Protein phosphorylation is a crucial post-translational modification that plays an important role in the regulation of cellular signaling processes. Site-specific quantitation of phosphorylation levels can help decipher the physiological functions of phosphorylation modifications under diverse physiological statuses. However, quantitative analysis of protein phosphorylation degrees is still a challenging task due to its dynamic nature and the lack of an internal standard simultaneously available for the samples differently prepared for various phosphorylation extents. In this study, stable-isotope dimethyl labeling coupled with phosphatase dephosphorylation (DM + deP) was tried to determine the site-specific degrees of phosphorylation in proteins. Firstly, quantitation accuracy of the (DM + deP) approach was confirmed using synthetic peptides of various simulated phosphorylation degrees. Afterwards, it was applied to evaluate the phosphorylation stoichiometry of milk caseins. The phosphorylation degree of Ser130 on α-S1-casein was also validated by absolute quantification with the corresponding synthetic phosphorylated and nonphosphorylated peptides under a selected reaction monitoring (SRM) mode. Moreover, this (DM + deP) method was used to detect the phosphorylation degree change of Ser82 on the Hsp27 protein of HepG2 cells caused by tert-butyl hydroperoxide (t-BHP) treatment. The results showed that the absolute phosphorylation degree obtained from the (DM + deP) approach was comparable with the relative quantitation resulting from stable-isotope dimethyl labeling coupled with TiO2 enrichment. This study suggested that the (DM + deP) approach is promising for absolute quantification of site-specific degrees of phosphorylation in proteins, and it may provide more convincing information than the relative quantification method.
Ligand-Targeted Delivery of Photosensitizers for Cancer Treatment
Authors: Gierlich Piotr , Mata Ana I. , Donohoe Claire , Brito Rui M. M. , Senge Mathias O. , Gomes-da-Silva Lígia C.
Published: November 2020

Photodynamic therapy (PDT) is a promising cancer treatment which involves a photosensitizer (PS), light at a specific wavelength for PS activation and oxygen, which combine to elicit cell death. While the illumination required to activate a PS imparts a certain amount of selectivity to PDT treatments, poor tumor accumulation and cell internalization are still inherent properties of most intravenously administered PSs. As a result, common consequences of PDT include skin photosensitivity. To overcome the mentioned issues, PSs may be tailored to specifically target overexpressed biomarkers of tumors. This active targeting can be achieved by direct conjugation of the PS to a ligand with enhanced affinity for a target overexpressed on cancer cells and/or other cells of the tumor microenvironment. Alternatively, PSs may be incorporated into ligand-targeted nanocarriers, which may also encompass multi-functionalities, including diagnosis and therapy. In this review, we highlight the major advances in active targeting of PSs, either by means of ligand-derived bioconjugates or by exploiting ligand-targeting nanocarriers.
Thiocoumarin Caged Nucleotides: Synthetic Access and Their Photophysical Properties
Authors: Ma Jiahui , Ripp Alexander , Wassy Daniel , Dürr Tobias , Qiu Danye , Häner Markus , Haas Thomas , Popp Christoph , Bezold Dominik , Richert Sabine , Esser Birgit , Jessen Henning J.
Published: November 2020

Photocages have been successfully applied in cellular signaling studies for the controlled release of metabolites with high spatio-temporal resolution. Commonly, coumarin photocages are activated by UV light and the quantum yields of uncaging are relatively low, which can limit their applications in vivo. Here, syntheses, the determination of the photophysical properties, and quantum chemical calculations of 7-diethylamino-4-hydroxymethyl-thiocoumarin (thio-DEACM) and caged adenine nucleotides are reported and compared to the widely used 7-diethylamino-4-hydroxymethyl-coumarin (DEACM) caging group. In this comparison, thio-DEACM stands out as a phosphate cage with improved photophysical properties, such as red-shifted absorption and significantly faster photolysis kinetics.
From Target-Oriented to Motif-Oriented: A Case Study on Nannocystin Total Synthesis
Authors: Zhang Weicheng
Published: November 2020

Natural product total synthesis is in essence target-oriented in that a set of organic transformations are orchestrated into a workable process, leading ultimately to the target molecule with a predefined architecture. For a bioactive lead, proof of synthetic viability is merely the beginning. Ensuing effort repurposes the initial synthesis for structural diversification in order to probe structure-activity relationship (SAR). Yet accessibility is not equal to flexibility; moving from convergency to divergency, it is not always feasible to explore the chemical space around a particular substructure of interest simply by tweaking an established route. In this situation, the motif-oriented strategy becomes a superior choice, which gives priority to synthetic flexibility at the concerned site such that a route is adopted only if it is capable of implementing diversification therein. This strategy was recently devised by Fürstner et al., enabling them to achieve total synthesis of both natural and non-natural nannocystins varied at an otherwise challenging position. The present review examines seven distinctive nannocystin total syntheses reported thus far and showcases the merits of conventional (target-oriented) as well as motif-oriented strategies, concluding that these two approaches complement each other and are both indispensable for natural product based drug discovery.
Autologous Materials in Regenerative Dentistry: Harvested Bone, Platelet Concentrates and Dentin Derivates
Authors: Bernardi Sara , Macchiarelli Guido , Bianchi Serena
Published: November 2020

The jawbone is a peculiar type of bone tissue, unique for its histological, anatomical and physiological characteristics. Therefore, a defect in the maxilla or in the mandible, because of pathological sequelae is difficult to prevent and to restore. Several biomaterials have been and are currently being developed to respond to the demands of regenerative medicine. A specific group of biomaterials used in regenerative dentistry is represented by the autologous materials. Platelet concentrates harvested bone and dentin derivates are indeed used in an attempt to minimise the alveolar resorption or in vertical ridge augmentation procedures or in sinus lift interventions. The aim of this review is to examine the properties of the above-listed materials, to compare them and to indicate eventual clinical applications.
Structure-Function Insights of Jaburetox and Soyuretox: Novel Intrinsically Disordered Polypeptides Derived from Plant Ureases
Authors: Grahl Matheus V. Coste , Lopes Fernanda Cortez , Martinelli Anne H. Souza , Carlini Celia R. , Fruttero Leonardo L.
Published: November 2020

Intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs) do not have a stable 3D structure but still have important biological activities. Jaburetox is a recombinant peptide derived from the jack bean (Canavalia ensiformis) urease and presents entomotoxic and antimicrobial actions. The structure of Jaburetox was elucidated using nuclear magnetic resonance which reveals it is an IDP with small amounts of secondary structure. Different approaches have demonstrated that Jaburetox acquires certain folding upon interaction with lipid membranes, a characteristic commonly found in other IDPs and usually important for their biological functions. Soyuretox, a recombinant peptide derived from the soybean (Glycine max) ubiquitous urease and homologous to Jaburetox, was also characterized for its biological activities and structural properties. Soyuretox is also an IDP, presenting more secondary structure in comparison with Jaburetox and similar entomotoxic and fungitoxic effects. Moreover, Soyuretox was found to be nontoxic to zebra fish, while Jaburetox was innocuous to mice and rats. This profile of toxicity affecting detrimental species without damaging mammals or the environment qualified them to be used in biotechnological applications. Both peptides were employed to develop transgenic crops and these plants were active against insects and nematodes, unveiling their immense potentiality for field applications.
Editorial for “Materials Chemistry” Section, in Journal Molecules
Authors: Malucelli Giulio
Published: November 2020

Dear colleagues and friends, it is a great pleasure to summarize the most significant successes achieved during 2019 in the “Materials Chemistry” Section (https://www [...]
Mechanochemical and Size Reduction Machines for Biorefining
Authors: Lomovskiy Igor , Bychkov Aleksey , Lomovsky Oleg , Skripkina Tatiana
Published: November 2020

In recent years, we have witnessed an increasing interest in the application of mechanochemical methods for processing materials in biomass refining techniques. Grinding and mechanical pretreatment are very popular methods utilized to enhance the reactivity of polymers and plant raw materials; however, the choice of devices and their modes of action is often performed through trial and error. An inadequate choice of equipment often results in inefficient grinding, low reactivity of the product, excess energy expenditure, and significant wear of the equipment. In the present review, modern equipment employing various types of mechanical impacts, which show the highest promise for mechanochemical pretreatment of plant raw materials, is examined and compared—disc mills, attritors and bead mills, ball mills, planetary mills, vibration and vibrocentrifugal mills, roller and centrifugal roller mills, extruders, hammer mills, knife mills, pin mills, disintegrators, and jet mills. The properly chosen type of mechanochemical activation (and equipment) allows an energetically and economically sound enhancement of the reactivity of solid-phase polymers by increasing the effective surface area accessible to reagents, reducing the amount of crystalline regions and the diffusion coefficient, disordering the supramolecular structure of the material, and mechanochemically reacting with the target substances.
Synthesis of a Series of Dual-Functional Chelated Titanate Bonding Agents and Their Application Performances in Composite Solid Propellants
Authors: Lin Guomin , Chang Yixue , Chen Yu , Zhang Wei , Ye Yanchun , Guo Yanwen , Jin Shaohua
Published: November 2020

Titanate-based bonding agents are a class of efficient bonding agents for improving the mechanical properties of composite solid propellants, a kind of special composite material. However, high solid contents often deteriorate the rheological properties of propellant slurry, which limits the application of bonding agents. To solve this problem, a series of long-chain alkyl chelated titanate binders, N-n-octyl-N, N-dihydroxyethyl-lactic acid-titanate (DLT-8), N-n-dodecyl-N, N-dihydroxyethyl-lactic acid-titanate (DLT-12), N-n-hexadecyl-N, N-Dihydroxyethyl-lactic acid-titanate (DLT-16), were designed and synthesized in the present work. The infrared absorption spectral changes of solid propellants caused by binder coating and adhesion degrees of the bonding agents on the oxidant surface were determined by micro-infrared microscopy (MIR) and X-ray photoelectron spectroscopy (XPS), respectively, to characterize the interaction properties of the bonding agents with oxidants, ammonium perchlorate (AP) and hexogen (RDX), in solid propellants. The further application tests suggest that the bonding agents can effectively interact with the oxidants and effectively improve the mechanical and rheological properties of the four-component hydroxyl-terminated polybutadiene (HTPB) composite solid propellants containing AP and RDX. The agent with longer bond chain length can improve the rheological properties of the propellant slurry more significantly, and the propellant of the best mechanical properties was obtained with DLT-12, consistent with the conclusion obtained in the interfacial interaction study. Our work has provided a new method for simultaneously improving the processing performance and rheological properties of propellants and offered an important guidance for the bonding agent design.
Role of Melatonin in Plant Tolerance to Soil Stressors: Salinity, pH and Heavy Metals
Authors: Moustafa-Farag Mohamed , Elkelish Amr , Dafea Mohamed , Khan Mumtaz , Arnao Marino B. , Abdelhamid Magdi T. , El-Ezz Aziz Abu , Almoneafy Abdlwareth , Mahmoud Ahmed , Awad Mahrous , Li Linfeng , Wang Yanhong , Hasanuzzaman Mirza , Ai Shaoying
Published: November 2020

Melatonin (MT) is a pleiotropic molecule with diverse and numerous actions both in plants and animals. In plants, MT acts as an excellent promotor of tolerance against abiotic stress situations such as drought, cold, heat, salinity, and chemical pollutants. In all these situations, MT has a stimulating effect on plants, fomenting many changes in biochemical processes and stress-related gene expression. Melatonin plays vital roles as an antioxidant and can work as a free radical scavenger to protect plants from oxidative stress by stabilization cell redox status; however, MT can alleviate the toxic oxygen and nitrogen species. Beyond this, MT stimulates the antioxidant enzymes and augments antioxidants, as well as activates the ascorbate–glutathione (AsA–GSH) cycle to scavenge excess reactive oxygen species (ROS). In this review, we examine the recent data on the capacity of MT to alleviate the effects of common abiotic soil stressors, such as salinity, alkalinity, acidity, and the presence of heavy metals, reinforcing the general metabolism of plants and counteracting harmful agents. An exhaustive analysis of the latest advances in this regard is presented, and possible future applications of MT are discussed.
Nickel Nanopillar Arrays Electrodeposited on Silicon Substrates Using Porous Alumina Templates
Authors: Bejide Matías , Contreras Patricio , Homm Pia , Duran Boris , García-Merino José Antonio , Rosenkranz Andreas , Denardin Juliano C. , del Río Rodrigo , Hevia Samuel A.
Published: November 2020

Nickel nanopillar arrays were electrodeposited onto silicon substrates using porous alumina membranes as a template. The characterization of the samples was done by scanning electron microscopy, X-ray diffraction, and alternating force gradient magnetometry. Ni nanostructures were directly grown on Si by galvanostatic and potentiostatic electrodeposition techniques in three remarkable charge transfer configurations. Differences in the growth mechanisms of the nanopillars were observed, depending on the deposition method. A high correlation between the height of the nanopillars and the charge synthesis was observed irrespective of the electrochemical technique. The magnetization measurements demonstrated a main dependence with the height of the nanopillars. The synthesis of Ni nanosystems with a controllable aspect ratio provides an effective way to produce well-ordered networks for wide scientific applications.
Development of Fish Immunity and the Role of β-Glucan in Immune Responses
Authors: Rodrigues Marianna V. , Zanuzzo Fábio S. , Koch João Fernando A. , de Oliveira Carlos Alberto F. , Sima Petr , Vetvicka Vaclav
Published: November 2020

Administration of β-glucans through various routes, including immersion, dietary inclusion, or injection, have been found to stimulate various facets of immune responses, such as resistance to infections and resistance to environmental stress. β-Glucans used as an immunomodulatory food supplement have been found beneficial in eliciting immunity in commercial aquaculture. Despite extensive research involving more than 3000 published studies, knowledge of the receptors involved in recognition of β-glucans, their downstream signaling, and overall mechanisms of action is still lacking. The aim of this review is to summarize and discuss what is currently known about of the use of β-glucans in fish.
A Comprehensive Review of Aminochalcones
Authors: Irfan Rimsha , Mousavi Shikufa , Alazmi Meshari , Saleem Rahman Shah Zaib
Published: November 2020

Chalcones, members of the flavonoid family, display a plethora of interesting biological activities including but not limited to antioxidant, anticancer, antimicrobial, anti-inflammatory, and antiprotozoal activities. The literature cites the synthesis and activity of a range of natural, semisynthetic, and synthetic chalcones. The current review comprehensively covers the literature on amino-substituted chalcones and includes chalcones with amino-groups at various positions on the aromatic rings as well as those with amino-groups containing mono alkylation, dialkylation, alkenylation, acylation, and sulfonylation. The aminochalcones are categorized according to their structure, and the corresponding biological activities are discussed as well. Some compounds showed high potency against cancer cells, microbes, and malaria, whereas others did not. The purpose of this review is to serve as a one-stop location for information on the aminochalcones reported in the literature in recent years.
Synthesis, Characterization, and Assessment of a CeO2@Nanoclay Nanocomposite for Enhanced Oil Recovery
Authors: Javad Nazarahari Mohammad , Khaksar Manshad Abbas , Moradi Siyamak , Shafiei Ali , Abdulazez Ali Jagar , Sajadi S. , Keshavarz Alireza
Published: November 2020

In this paper, synthesis and characterization of a novel CeO2/nanoclay nanocomposite (NC) and its effects on IFT reduction and wettability alteration is reported in the literature for the first time. The NC was characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), and EDS MAP. The surface morphology, crystalline phases, and functional groups of the novel NC were investigated. Nanofluids with different concentrations of 100, 250, 500, 1000, 1500, and 2000 ppm were prepared and used as dispersants in porous media. The stability, pH, conductivity, IFT, and wettability alternation characteristics of the prepared nanofluids were examined to find out the optimum concentration for the selected carbonate and sandstone reservoir rocks. Conductivity and zeta potential measurements showed that a nanofluid with concentration of 500 ppm can reduce the IFT from 35 mN/m to 17 mN/m (48.5% reduction) and alter the contact angle of the tested carbonate and sandstone reservoir rock samples from 139° to 53° (38% improvement in wettability alteration) and 123° to 90° (27% improvement in wettability alteration), respectively. A cubic fluorite structure was identified for CeO2 using the standard XRD data. FESEM revealed that the surface morphology of the NC has a layer sheet morphology of CeO2/SiO2 nanocomposite and the particle sizes are approximately 20 to 26 nm. TGA analysis results shows that the novel NC has a high stability at 90 °C which is a typical upper bound temperature in petroleum reservoirs. Zeta potential peaks at concentration of 500 ppm which is a sign of stabilty of the nanofluid. The results of this study can be used in design of optimum yet effective EOR schemes for both carbobate and sandstone petroleum reservoirs.