bluuebunny/crossref_metadata_2025 huggingface dataset:
Endogenous Galectin-1 Modulates Cell Biological Properties of Immortalized Retinal Pigment Epithelial Cells In Vitro
Authors: Liesenhoff Caspar , Paulus Simon Martin , Havertz Caroline , Geerlof Arie , Priglinger Siegfried , Priglinger Claudia Sybille , Ohlmann Andreas
Published: August 2023

In the eye, an increase in galectin-1 is associated with various chorioretinal diseases, in which retinal pigment epithelium (RPE) cells play a crucial role in disease development and progression. Since little is known about the function of endogenous galectin-1 in these cells, we developed a galectin-1-deficient immortalized RPE cell line (ARPE-19-LGALS1−/−) using a sgRNA/Cas9 all-in-one expression vector and investigated its cell biological properties. Galectin-1 deficiency was confirmed by Western blot analysis and immunocytochemistry. Cell viability and proliferation were significantly decreased in ARPE-19-LGALS1−/− cells when compared to wild-type controls. Further on, an increased attachment of galectin-1-deficient RPE cells was observed by cell adhesion assay when compared to control cells. The diminished viability and proliferation, as well as the enhanced adhesion of galectin-1-deficient ARPE-19 cells, could be blocked, at least in part, by the additional treatment with human recombinant galectin-1. In addition, a significantly reduced migration was detected in ARPE-19-LGALS1−/− cells. In comparison to control cells, galectin-1-deficient RPE cells had enhanced expression of sm-α-actin and N-cadherin, whereas expression of E-cadherin showed no significant alteration. Finally, a compensatory expression of galectin-8 mRNA was observed in ARPE-19-LGALS1−/− cells. In conclusion, in RPE cells, endogenous galectin-1 has crucial functions for various cell biological processes, including viability, proliferation, migration, adherence, and retaining the epithelial phenotype.
Application of Multi-Perspectives in Tea Breeding and the Main Directions
Authors: Li Haozhen , Song Kangkang , Zhang Xiaohua , Wang Di , Dong Shaolin , Liu Ying , Yang Long
Published: August 2023

Tea plants are an economically important crop and conducting research on tea breeding contributes to enhancing the yield and quality of tea leaves as well as breeding traits that satisfy the requirements of the public. This study reviews the current status of tea plants germplasm resources and their utilization, which has provided genetic material for the application of multi-omics, including genomics and transcriptomics in breeding. Various molecular markers for breeding were designed based on multi-omics, and available approaches in the direction of high yield, quality and resistance in tea plants breeding are proposed. Additionally, future breeding of tea plants based on single-cellomics, pangenomics, plant–microbe interactions and epigenetics are proposed and provided as references. This study aims to provide inspiration and guidance for advancing the development of genetic breeding in tea plants, as well as providing implications for breeding research in other crops.
Scopoletin Reactivates Latent HIV-1 by Inducing NF-κB Expression without Global T Cell Activation
Authors: Zhu Yuqi , Jiang Zhengtao , Liu Lin , Yang Xinyi , Li Min , Cheng Yipeng , Xu Jianqing , Yin Chunhua , Zhu Huanzhang
Published: August 2023

Reversing HIV-1 latency promotes the killing of infected cells and is essential for cure strategies. However, current latency-reversing agents (LRAs) are not entirely effective and safe in activating latent viruses in patients. In this study, we investigated whether Scopoletin (6-Methoxy-7-hydroxycoumarin), an important coumarin phytoalexin found in plants with multiple pharmacological activities, can reactivate HIV-1 latency and elucidated its underlying mechanism. Using the Jurkat T cell model of HIV-1 latency, we found that Scopoletin can reactivate latent HIV-1 replication with a similar potency to Prostratin and did so in a dose- and time-dependent manner. Moreover, we provide evidence indicating that Scopoletin-induced HIV-1 reactivation involves the nuclear factor kappa B (NF-κB) signaling pathway. Importantly, Scopoletin did not have a stimulatory effect on T lymphocyte receptors or HIV-1 receptors. In conclusion, our study suggests that Scopoletin has the potential to reactivate latent HIV-1 without causing global T-cell activation, making it a promising treatment option for anti-HIV-1 latency strategies.
Insights into the Inhibitory Mechanisms of the Covalent Drugs for DNMT3A
Authors: Yang Wei , Zhuang Jingyuan , Li Chen , Bai Chen , Cheng Guijuan
Published: August 2023

The perturbations of DNA methyltransferase 3 alpha (DNMT3A) may cause uncontrolled gene expression, resulting in cancers and tumors. The DNMT inhibitors Azacytidine (AZA) and Zebularine (ZEB) inhibit the DNMT family with no specificities, and consequently would bring side effects during the treatment. Therefore, it is vital to understand the inhibitory mechanisms in DNMT3A to inform the new inhibitor design for DNMTs. Herein, we carried out molecular dynamics (MD) and quantum mechanics/molecular mechanics (QM/MM) simulations to investigate the inhibitory mechanisms of the AZA and ZEB. The results were compared to the methyl transfer of cytosine. We showed how the AZA might stop the methyl transfer process, whereas the ZEB might be stuck in a methyl-transferred intermediate (IM3). The IM3 state then fails the elimination due to the unique protein dynamics that result in missing the catalytic water chain. Our results brought atomic-level insights into the mechanisms of the two drugs in DNMT3A, which could benefit the new generation of drug design for the DNMTs.
Explanation of Student Attendance AI Prediction with the Isabelle Infrastructure Framework
Authors: Kammüller Florian , Satija Dimpy
Published: August 2023

Right from the beginning, attendance has played an important role in the education systems, not only in student success but in the overall interest of the matter. Although all schools try to accentuate good attendance, still some schools find it hard to achieve the required level (96% in UK) of average attendance. The most productive way of increasing the pupils′ attendance rate is to predict when it is going to go down, understand the reasons—why it happened—and act on the affecting factors so as to prevent it. Artificial intelligence (AI) is an automated machine learning solution for different types of problems. Several machine learning (ML) models like logistic regression, decision trees, etc. are easy to understand; however, complicated (Neural Network, BART etc.) ML models are not transparent but are black-boxes for humans. It is not always evident how machine intelligence arrived at a decision. However, not always, but in critical applications it is important that humans can understand the reasons for such decisions. In this paper, we present a methodology on the application example of pupil attendance for constructing explanations for AI classification algorithms. The methodology includes building a model of the application in the Isabelle Insider and Infrastructure framework (IIIf) and an algorithm (PCR) that helps us to obtain a detailed logical rule to specify the performance of the black-box algorithm, hence allowing us to explain it. The explanation is provided within the logical model of the IIIf, thus is suitable for human audiences. It has been shown that the RR-cycle of IIIf can be adapted to provide a method for iteratively extracting an explanation by interleaving attack tree analysis with precondition refinement, which finally yields a general rule that describes the decision taken by a black-box algorithm produced by Artificial intelligence.
Associations of Depressive Symptoms with Subjective Cognitive Decline in Elderly People—A Cross-Sectional Analysis from the AgeWell.de-Study
Authors: Zöllinger Isabel , Bauer Alexander , Blotenberg Iris , Brettschneider Christian , Buchholz Maresa , Czock David , Döhring Juliane , Escales Catharina , Fankhaenel Thomas , Frese Thomas , Hoffmann Wolfgang , Kaduszkiewicz Hanna , König Hans-Helmut , Luppa Melanie , Oey Anke , Pabst Alexander , Sanftenberg Linda , Thyrian Jochen René , Weiss Julian , Wendel Flora , Wiese Birgitt , Riedel-Heller Steffi G. , Gensichen Jochen
Published: August 2023

To develop effective dementia prevention strategies, it is necessary to understand risk factors, associated factors and early signs of dementia. Subjective cognitive decline (SCD) is the earliest form of dementia. The aim of this study is to assess depression as a factor that is significantly associated with SCD. The data of 1030 general practitioner patients from the AgeWell.de-study (60–77 years; CAIDE dementia risk score ≥ 9) were analysed. A descriptive analysis was conducted using validated instruments like the Geriatric depression scale (GDS), Lubben social network scale (LSNS-6) and education classes according to CASMIN (Comparative Analysis of Social Mobility in Industrial Nations). A multivariate regression model with the dependent variable SCD was calculated. Of the 1030 participants, 5.9% had depressive symptoms and 31.3% SCD. The group with depressive symptoms showed significantly higher body-mass-index (p = 0.005), lower education class (p = 0.022), lower LSNS-6 score (p < 0.001), higher sports activity (p < 0.001), and more sleeping problems (p = 0.026). In the regression model a higher GDS-score [Odds ratio (OR): 1.219 (p < 0.001)], more sleeping problems [OR: 1.550 (p = 0.017)] and higher education class [middle/high: OR: 1.474/1.875 (p = 0.037/0.004)] were significantly associated with SCD. This study identified depressive symptoms, sleeping problems, and higher education classes as factors associated with SCD, which can represent an early form of dementia.
Association between Polymorphism eNOS4, tPA, Factor V Leiden, Prothrombin, and Methylenetetrahydrofolate Reductase and the Occurrence of Legg–Calvé–Perthes Disease
Authors: Matuszewska Anna , Sygacz Oliwer , Matuszewski Łukasz , Stec Szymon , Grzegorzewski Andrzej , Gągała Jacek
Published: August 2023

Background. Legg–Calvé–Perthes (LCPD) disease is a complex condition affecting the femoral head’s epiphysis in children. It occurs with a prevalence ranging from 0.4 to 29.0 cases per 100,000 children under the age of 15. It involves various factors, including genes associated with coagulation and fibrinolysis, pro-inflammatory factors, and vasoactive substances. Methods. We investigated the relationship between genetic mutations associated with coagulation and vascular disorders and the occurrence of LCPD in Polish patients. We performed a study involving 25 patients with LCPD and 100 healthy controls. All subjects were genotyped for eNOS4, Factor V Leiden, prothrombin, tPA25, and MTHFR polymorphism. Results. The analysis revealed that the frequencies of eNOS4 genotypes were significantly different in LCPD patients than in the control group (p = 0.018). The frequencies of 4a allele were significantly higher in patients with LCPD than in the healthy population (26% vs. 9%, p = 0.0012). There were no significant differences in genotype and allele frequencies for Factor V Leiden, prothrombin tPA 25, and MTHFR gene polymorphisms between patients with LCPD and the controls. Conclusions. Genotype and allele frequencies of eNOS4 were significantly higher in patients with LCPD. These findings suggest a potential association between the eNOS gene polymorphism and an increased risk of developing LCPD.
Initial Temporal Muscle Thickness and Area: Poor Predictors of Neurological Outcome in Aneurysmal Subarachnoid Hemorrhage in a Central European Patient Cohort
Authors: Karadag Cihat , Kamp Marcel A. , Fischer Igor , Boogaarts Hieronymus D. , Beseoglu Kerim , Muhammad Sajjad , Cornelius Jan F. , Hofmann Björn B.
Published: August 2023

The temporalis muscle area (TMA) has been proclaimed as a surrogate parameter for estimating skeletal muscle mass. Pilot studies in Asian populations suggested temporal muscle thickness (TMT) and TMA as prognostic factors for neurological outcomes in aneurysmal subarachnoid hemorrhage (aSAH) patients. This study aimed to validate these findings in a larger European patient cohort. We retrospectively analyzed age, sex, aneurysm location, treatment, World Federation of Neurosurgical Societies (WFNS) grade, Fisher score, and modified Rankin Score (mRS) at six months in patients with aSAH. TMT and TMA measurements were obtained from initial native CT scans. Logistic regression with the dichotomized six-month mRS as the outcome incorporating TMT, weighted average of TMT, or TMA as predictors was performed. Of the included 478 patients, 66% were female, the mean age was 56, and 48% of patients had an mRS of three to six after six months. The mean TMT at the level of the Sylvian fissure was 5.9 (±1.7) mm in males and 4.8 (±1.8) mm in females. The mean TMA was 234.5 (±107.9) mm2 in females and 380 (±134.1) mm2 in males. WFNS grade (p < 0.001), Fisher score (p < 0.001), and age (p < 0.05) correlated significantly with the mRS after six months. No correlation was found between mRS after six months and the TMT at the Sylvian fissure (p = 0.3), the weighted average of TMT (p = 0.1), or the TMA (p = 0.1). In this central European patient cohort of 478 individuals, no significant associations were found between TMT/TMA and neurological outcomes following aSAH. Further prospective studies in diverse patient populations are necessary to determine the prognostic value of TMA and TMT in aSAH patients.
Aberrant HPO Axis Alterations and Autoimmune Abnormalities in PCOS Patients with DOR: A Retrospective Analysis
Authors: Geng Xueying , He Zhihong , Bao Zhouzhou , Di Wen , Gu Zhuowei
Published: August 2023

Background: There is a group of polycystic ovary syndrome (PCOS) patients in clinic who have diminished ovarian reserve (DOR) in combination. This study was designed to evaluate the differences in glucolipid metabolism, hypothalamic–pituitary–ovarian (HPO) axis-related parameters, and autoimmune antibodies in PCOS patients with and without DOR. Methods: A total of 2307 PCOS patients, including 1757 patients with PCOS alone and 550 patients who have both PCOS and DOR, were enrolled in this retrospective study. Parameters of glucolipid metabolism, HPO axis-related parameters, and autoimmune antibodies were measured and analyzed. Results: The prevalence of DOR among all patients with PCOS was 23.84%. Many HPO axis-related parameters, such as follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) were significantly different in PCOS with DOR compared with PCOS without DOR. The FSH levels were positively correlated with LH, testosterone (T), and androstenedione (AD) levels, but had no association with glucolipid metabolism after adjusting for body mass index (BMI). Moreover, anti-ovarian antibody (AOAb) and anti-21-OH antibody (21-OHAb) levels were significantly elevated in PCOS patients with DOR. Conclusions: PCOS patients with DOR showed more chaotic HPO axis hormone levels and elevated autoimmune antibodies, suggesting that autoimmune factors may be the cause of DOR in women with PCOS.
Impact of Advanced Age on the Incidence of Major Adverse Cardiovascular Events in Patients with Type 2 Diabetes Mellitus and Stable Coronary Artery Disease in a Real-World Setting in Spain
Authors: González-Juanatey Carlos , Anguita-Sánchez Manuel , Barrios Vivencio , Núñez-Gil Iván , Gómez-Doblas Juan José , García-Moll Xavier , Lafuente-Gormaz Carlos , Rollán-Gómez María Jesús , Peral-Disdier Vicente , Martínez-Dolz Luis , Rodríguez-Santamarta Miguel , Viñolas-Prat Xavier , Soriano-Colomé Toni , Muñoz-Aguilera Roberto , Plaza Ignacio , Curcio-Ruigómez Alejandro , Orts-Soler Ernesto , Segovia-Cubero Javier , Fanjul Víctor , Marín-Corral Judith , Cequier Ángel , SAVANA Research Group
Published: August 2023

Patients with type 2 diabetes mellitus (T2DM) and coronary artery disease (CAD) without myocardial infarction (MI) or stroke are at high risk for major cardiovascular events (MACEs). We aimed to provide real-world data on age-related clinical characteristics, treatment management, and incidence of major cardiovascular outcomes in T2DM-CAD patients in Spain from 2014 to 2018. We used EHRead® technology, which is based on natural language processing and machine learning, to extract unstructured clinical information from electronic health records (EHRs) from 12 hospitals. Of the 4072 included patients, 30.9% were younger than 65 years (66.3% male), 34.2% were aged 65–75 years (66.4% male), and 34.8% were older than 75 years (54.3% male). These older patients were more likely to have hypertension (OR 2.85), angina (OR 1.64), heart valve disease (OR 2.13), or peripheral vascular disease (OR 2.38) than those aged <65 years (p < 0.001 for all comparisons). In general, they were also more likely to receive pharmacological and interventional treatments. Moreover, these patients had a significantly higher risk of MACEs (HR 1.29; p = 0.003) and ischemic stroke (HR 2.39; p < 0.001). In summary, patients with T2DM-CAD in routine clinical practice tend to be older, have more comorbidities, are more heavily treated, and have a higher risk of developing MACE than is commonly assumed from clinical trial data.
Enhancing Polymethyl Methacrylate Prostheses for Cranioplasty with Ti mesh Inlays
Authors: Nayak Gargi Shankar , Palkowski Heinz , Carradò Adele
Published: August 2023

Biocompatible polymers such as polymethyl methacrylate (PMMA), despite fulfilling biomedical aspects, lack the mechanical strength needed for hard-tissue implant applications. This gap can be closed by using composites with metallic reinforcements, as their adaptable mechanical properties can overcome this problem. Keeping this in mind, novel Ti-mesh-reinforced PMMA composites were developed. The influence of the orientation and volume fraction of the mesh on the mechanical properties of the composites was investigated. The composites were prepared by adding Ti meshes between PMMA layers, cured by hot-pressing above the glass transition temperature of PMMA, where the interdiffusion of PMMA through the spaces in the Ti mesh provided sufficient mechanical clamping and adhesion between the layers. The increase in the volume fraction of Ti led to a tremendous improvement in the mechanical properties of the composites. A significant anisotropic behaviour was analysed depending on the direction of the mesh. Furthermore, the shaping possibilities of these composites were investigated via four-point bending tests. High shaping possibility was found for these composites when they were shaped at elevated temperature. These promising results show the potential of these materials to be used for patient-specific implant applications.
A Comparative Study of a Machine Learning Approach and Response Surface Methodology for Optimizing the HPT Processing Parameters of AA6061/SiCp Composites
Authors: El-Garaihy Waleed H. , Alateyah Abdulrahman I. , Shaban Mahmoud , Alsharekh Mohammed F. , Alsunaydih Fahad Nasser , El-Sanabary Samar , Kouta Hanan , El-Taybany Yasmine , Salem Hanadi G.
Published: August 2023

This work investigates the efficacy of high-pressure torsion (HPT), as a severe plastic deformation mechanism for processing plain and silicon-carbide-reinforced AA6061, with the broader objective of using the technique for improving the properties of lightweight materials for a range of objectives. The interactions between input variables, such as the pressure and equivalent strain (εeq) applied during HPT processing, and the presence of SiCp and response variables, like the relative density, grain refinement, homogeneity of the structure, and the mechanical properties of the AA6061 aluminum matrix, were investigated. Hot compaction (HC) of the mixed powders followed by HPT were employed to produce AA6061 discs with and without 15% SiCp. The experimental findings were then analyzed statistically using the response surface methodology (RSM) and a machine learning (ML) approach to predict the output variables and to optimize the input parameters. The optimum combination of HPT process parameters was confirmed by the genetic algorithm (GA) and ML approaches. Furthermore, the constructed ML and RSM models were validated experimentally by HPT processing the same material under new conditions not fed into the models and comparing the experimental results to those predicted by the model. From the ML and RSM models, it was found that processing the AA6061/SiCp composite HPT via four revolutions at 3 GPa produced the highest mechanical properties coupled with significant grain refinement compared to the HC condition. ML analysis revealed that the equivalent strain induced by the number of revolutions was the most effective parameter for grain refinement, whereas the presence of SiCp played the highest role in improving both the hardness values and the compressive strength of the AA6061 matrices.
GERD as a Complication of Laparoscopic Sleeve Gastrectomy for the Treatment of Obesity: A Systematic Review and Meta-Analysis
Authors: Znamirowski Przemysław , Kołomańska Magdalena , Mazurkiewicz Robert , Tymchyshyn Oksana , Nawacki Łukasz
Published: August 2023

Introduction: The incidence of obesity is increasing in developed societies, and surgical treatment is one treatment option. The most common surgical treatment for obesity is laparoscopic sleeve gastrectomy (LSG). Gastroesophageal reflux disease (GERD) is a complication of both obesity and the surgical treatment of obesity. Materials and methods: In this study, the PubMed database was searched using the keywords “GERD” and “bariatric surgery”, and 987 papers published between 1 July 2017 and 30 June 2022 were retrieved. Results: Nine papers met the inclusion criteria and were included in the meta-analysis. The articles were analyzed for the de novo occurrence of GERD after the treatment of its symptoms, the occurrence of erosive esophagitis, and Barrett’s esophagus. In addition, interesting conclusions are presented from the papers that did not meet the inclusion criteria but shed light on the pathophysiology of GERD in obese patients undergoing LSG. Conclusion: In conclusion, the authors draw attention to the need for endoscopic surveillance in patients undergoing LSG, even in the absence of clinical signs of GERD.
Utilizing the Systemic Immune-Inflammation Index and Blood-Based Biomarkers in Association with Treatment Responsiveness amongst Patients with Treatment-Resistant Bipolar Depression
Authors: Decker Kyle , Murata Stephen , Baig Nausheen , Hasan Sakibur , Halaris Angelos
Published: August 2023

(1) Background: Inflammation is associated with depressive illness and treatment resistance. This study assessed a novel inflammatory index, the Systemic Immune-Inflammation Index (SII), in patients diagnosed with treatment-resistant bipolar depression (TRBDD) before and after treatment with escitalopram (ESC) and celecoxib (CBX) add-on or ESC and placebo (PBO), and compared them to healthy control (HC) subjects. (2) Methods: This is a secondary biological analysis from a double-blind randomized placebo-controlled trial of CBX augmentation in TRBDD. Our subsample with available complete blood count (CBC) data included 52 TRBDD subjects, randomized into an ESC + CBX, (n = 29), an ESC + PBO arm (n = 23), and an HC group (n = 32). SII was calculated from the CBC with differential (SII = platelets x neutrophils/lymphocytes) at baseline and end of treatment (8 weeks). Blood inflammation biomarkers, growth factors, and kynurenine metabolites were determined at both timepoints. Depressive symptom severity was the primary outcome, using the HAMD-17 rating scale score to quantitate treatment response and remission rates. (3) Results: Baseline SII did not discriminate TRBDD from HC, nor was it associated with HAMD-17 score at any timepoint, although it was significantly associated with lower baseline VEGF (p = 0.011) and higher week 8 levels of IL1-β (p = 0.03) and CRP (p = 0.048). Post-treatment HAMD-17 was not independently predicted using baseline SII unless an interaction with age was present (p = 0.003 was included), even after relevant adjustments. A similar effect was seen with baseline neutrophils. (4) Conclusions: While SII was not an independent predictor of treatment outcome, elevated baseline SII was a predictor of poor treatment response amongst older patients with TRBDD.
Territorialising Local Food Systems for an Agroecological Transition in Latin America
Authors: Levidow Les , Sansolo Davis , Schiavinatto Mônica
Published: August 2023

An agroecological transition can enhance resilience by several means, e.g., managing ecological relationships through agroecosystems, enhancing farmers’ knowledge of natural resources, recycling those resources, maintaining biodiversity, and thus, flexibly adapting to environmental stresses. However, the hegemonic agri-food system has been continuing its capitalist transition, thereby undermining agroecological methods and deterritorialising social bonds. Facing this pervasive threat, an agroecological transition needs a greater convergence between agroecological production and a solidarity economy (economia solidaria or EcoSol in Latin America). Their convergence can be called EcoSol-agroecology, based on short food supply chains (called circuitos cortos there). These efforts develop territorial markets, generate more stable livelihoods, and thus keep producers on the land. In our study, each research team collaborated with an EcoSol-agroecology network to develop Participatory Action Research methods. The COVID-19 pandemic disrupted their circuitos cortos, stimulating creative adaptations or alternatives, alongside demands for policy support measures. These networks have regionally territorialised local initiatives, while also confronting obstacles from the hegemonic system. Although socioecological resilience often means a system’s capacity to bounce back, here it has meant bouncing forwards through new opportunities for solidaristic livelihoods and bonds. EcoSol-agroecology networks, agri-extensionists, and researchers have jointly developed such counter-hegemonic strategies, as illustrated by the case studies here.
Preservation of Cultural Landscape as a Tool for the Sustainable Development of Rural Areas: The Case of Mani Peninsula in Greece
Authors: Oikonomopoulou Eleni , Delegou Ekaterini T. , Sayas John , Vythoulka Anastasia , Moropoulou Antonia
Published: August 2023

A cultural landscape is a complex and integrating concept with both material–physical and immaterial substance. Nevertheless, even today, the strategies for the protection and promotion of cultural landscapes are concentrated only at their material cultural elements, separated from their immaterial existence, or even from the natural environment in which they are placed. This study investigates the correlation of the tangible historical and natural heritage of a cultural landscape with its intangible content, as a spatial planning tool for its sustainable development. The proposed methodology was applied in the region of the Mani Peninsula, located in the southern Peloponnese, in Greece. During the documentation stage, literature research and fieldwork provided descriptive information, which was classified through standardization processes. GIS management and analysis procedures were used among the different layers of data of the current preservation state and the existing development frameworks for the study area. New thematic cultural routes are proposed to connect tangible cultural heritage and environmental values of the region under study, with the landscape’s geomorphological characteristics and intangible content. Protected areas are also proposed for the protection of monuments and sites of historical or natural importance. The results of this study demonstrate that, through integrated strategic planning for the development of cultural activities and networks, which incorporates the principles of spatial and urban planning, not only is the protection of the natural and cultural wealth of the region achieved, but also a balanced economic development and social cohesion, which ultimately leads to sustainable development.
Hydrogen Peroxide Diffusion through Dental Tissues—In Vitro Study
Authors: Dias Susana , Casqueiro Leonor , Pereira Ruben , Silveira João , Mata António , Marques Duarte
Published: August 2023

Whitening products commonly utilize hydrogen peroxide (HP) as an active principle, which can penetrate dental tissues with potential side effects due to its low molecular weight. This study aimed to evaluate the HP diffusion of two in-office whitening products, namely 6% VivaStyle Paint On Plus (VS) and Opalescence Boost 40% (OP), in different tooth types. Additionally, the influence of the area of exposure, dental tissue thickness and pulp chamber volume was assessed. Each group consisted of eighteen intact anterior (A), premolar (PM) and molar (M) human teeth, and a positive pulpal pressure model was employed. The samples were analyzed using spectrophotometry, and results were expressed as the mean and 95% confidence interval. Statistical tests and linear regression models were appropriately applied at α = 5%. The total HP (µg) retrieved was as follows: VS-A, 1.333 [1.214, 1.452]; OP-A, 1.538 [1.457, 1.620]; VS-PM, 1.208 [1.123, 1.291]; OP-PM, 3.628 [3.401, 3.855]; VS-M, 2.560 [2.297, 2.823]; and OP-M, 4.197 [3.997, 4.396], with statistically significant differences in diffusion kinetics between whitening products for PM and M. Several HP concentrations attained a minimum cytotoxicity value of 2.22 µg/mL. The regression model shows that OP exposed the pulp chamber to 1.421 µg of HP more than that of VS. Different whitening products can cause cytotoxic HP concentrations in the pulp chamber, with a higher risk observed in molars.
Metal–Ceramic Compatibility in Dental Restorations According to the Metallic Component Manufacturing Procedure
Authors: Dawod Nazem , Miculescu Marian , Antoniac Iulian Vasile , Miculescu Florin , Agop-Forna Doriana
Published: August 2023

In terms of production technology, metal–ceramic systems for dental restorations comply with a concrete algorithm, the efficiency of which is always dependent on the applications for which they are intended. The first stage involves obtaining metal support, followed by firing the ceramic on the surface of the metal to meet the list of functional and aesthetic requirements of a future restoration. The compatibility of the two materials—the metal component and the ceramic component—must be ensured in several respects: chemical compatibility, thermo–chemical compatibility, and mechanical compatibility. Thus, there is a need to simulate the thermal behavior of the metal–ceramic couple in its processing to achieve appropriate dental prostheses. In this study, three types of Co–Cr metal frames were manufactured using three different production technologies: conventional casting, milling (CAM), and selective laser melting (SLM). Composition analyses, scanning electron microscopy (SEM), and microstructural analyses of the metal–ceramic interface for each type of production technology, as well as the determination of the hardness and the thermal expansion coefficients of experimental materials and three-point bending tests, were carried out in this study. Considering all these aspects, we demonstrated the influence of the technology of producing the metallic part of the metal–ceramic bonding process in dental prostheses.
Seamless Weft Knit Vest with Integrated Needle Sensing Zone for Monitoring Shoulder Movement: A First Methodological Study
Authors: Sun Fei , Dong Zhijia , Din Yuqin , Cong Honglian , Ma Pibo
Published: August 2023

The integration of textile-based flexible sensors and electronic devices has accelerated the development of wearable textiles for posture monitoring. The complexity of the processes required to create a complete monitoring product is currently reflected in three main areas. The first is the sensor production process, which is complex. Second, the integration of the sensor into the garment requires gluing or stitching. Finally, the production of the base garment requires cutting and sewing. These processes deteriorate the user experience and hinder the commercial mass production of wearable textiles. In this paper, we knitted a one-piece seamless knitted vest (OSKV) utilizing the one-piece seamless knitting technique and positioned an embedded needle sensing zone (EHSZ) with good textile properties and electrical performance for monitoring human shoulder activity. The EHSZ was knitted together with the OSKV, eliminating the need for an integration process. The EHSZ exhibited good sensitivity (GF = 2.23), low hysteresis (0.29 s), a large stretch range (200%), and excellent stability (over 300 cycles), satisfying the requirement to capture a wide range of deformation signals caused by human shoulder movements. The OSKV described the common vest process structure without the stitching process. Furthermore, OSKV fulfilled the demand for seamless and trace-free monitoring while effortlessly and aesthetically satisfying the knitting efficiency of commercial garments.
Effect of High-Speed Sintering on the Optical Properties, Microstructure, and Phase Distribution of Multilayered Zirconia Stabilized with 5 mol% Yttria
Authors: Cho Mi-Hyang , Seol Hyo-Joung
Published: August 2023

As dental 5 mol% yttria-stabilized (5Y-) zirconia demand high esthetics, it is necessary to clarify how the optical properties are affected by high-speed sintering, which is not yet fully understood. Our study aimed to investigate the effect of high-speed sintering on the translucency and opalescence parameters (TP and OP, respectively), as well as their related microstructure and phase distribution, using two types of multilayered 5Y-zirconia. Multilayered 5Y-zirconia (Cercon xt ML, Lava Esthetic) were cut layer-by-layer, followed by conventional and high-speed sintering. The TP and OP values were subsequently obtained using a spectrophotometer, and field emission scanning electron microscopy images were used to analyze the average grain size. The phase fractions were analyzed using X-ray diffraction. Regardless of the zirconia type, the TP was slightly lowered by high-speed sintering in all the layers except the dentin layer (DL) for Lava Esthetic (p < 0.05). The OP decreased by high-speed sintering in the DL for Cercon xt ML and in all the layers for Lava Esthetic (p < 0.05). The decrease in translucency after high-speed sintering was attributed to a decrease in the yttria-rich t’-phase with low tetragonality, along with an increase in the yttria-lean t-phase with high tetragonality.
Precision Grinding Technology of Silicon Carbide (SiC) Ceramics by Longitudinal Torsional Ultrasonic Vibrations
Authors: Ye Zejiu , Wen Xu , Wan Weiqiang , Liu Fuchu , Bai Wei , Xu Chao , Chen Hui , Gong Pan , Han Guangchao
Published: August 2023

Silicon carbide (SiC) ceramic material has become the most promising third-generation semiconductor material for its excellent mechanical properties at room temperature and high temperature. However, SiC ceramic machining has serious tool wear, low machining efficiency, poor machining quality and other disadvantages due to its high hardness and high wear resistance, which limits the promotion and application of such materials. In this paper, comparison experiments of longitudinal torsional ultrasonic vibration grinding (LTUVG) and common grinding (CG) of SiC ceramics were conducted, and the longitudinal torsional ultrasonic vibration grinding SiC ceramics cutting force model was developed. In addition, the effects of ultrasonic machining parameters on cutting forces, machining quality and subsurface cracking were investigated, and the main factors and optimal parameters affecting the cutting force improvement rate were obtained by orthogonal tests. The results showed that the maximum improvement of cutting force, surface roughness and subsurface crack fracture depth by longitudinal torsional ultrasonic vibrations were 82.59%, 22.78% and 30.75%, respectively. A longitudinal torsional ultrasonic vibrations cutting force prediction model containing the parameters of tool, material properties and ultrasound was established by the removal characteristics of SiC ceramic material, ultrasonic grinding principle and brittle fracture theory. And the predicted results were in good agreement with the experimental results, and the maximum error was less than 15%. The optimum process parameters for cutting force reduction were a spindle speed of 22,000 rpm, a feed rate of 600 mm/min and a depth of cut of 0.011 mm.
A Cyclic Calibration Method of Milling Force Coefficients Considering Elastic Tool Deformation
Authors: Yang Chang , Yu Rong , Jiang Shanglei
Published: August 2023

In metal-cutting technology, milling plays an important role in the product development cycle. The accurate modeling and prediction of milling forces have always been research hotspots in this field. The mechanical model based on unit-cutting force coefficients has a high prediction accuracy of cutting forces, and it is therefore widely used in the modeling of milling forces. The calibration of the cutting force coefficients can be realized by linear regression analysis of the measured average milling forces, but it needs to carry out multiple groups of variable feed slot milling experiments with full radial depth of cut, and it cannot well represent the interaction condition in peripheral milling with a non-full radial depth of cut. In peripheral milling, the tool will inevitably deform under the influence of cutting force in the direction perpendicular to the machining surface. If the force-induced deformation is ignored, the calibration of the cutting force coefficients will be out of alignment. For this non-slot milling condition where one side of the tool is mainly stressed, a cyclic calibration method for milling force coefficients considering elastic deformation along the axial contact range is proposed. Firstly, the cutting force coefficients are preliminarily calibrated by the experimental data, and, secondly, tool deformation is calculated through a preliminarily calibrated cutting force model cycle until convergence, before cutting boundaries are updated. The cutting force coefficient is then calibrated again, and it is brought back to the cantilever beam model in order to calculate the tool deformation again. The above process is repeated until the cutting force coefficient is convergent. Finally, the cutting force coefficients are obtained in order to predict and model the milling forces.
Experimental Investigation on the Flow Boiling of Two Microchannel Heat Sinks Connected in Parallel and Series for Cooling of Multiple Heat Sources
Authors: Jiang Zhengyong , Song Mengjie , Shen Jun , Zhang Long , Zhang Xuan , Lin Shenglun
Published: August 2023

Cooling methods for multiple heat sources with high heat flux have rarely been reported, but such situations threaten the stable operation of electronic devices. Therefore, in this paper, the use of two microchannel heat sinks is proposed, with and without grooves, labeled Type A and Type B, respectively. Experimental investigations on the flow boiling of two microchannel heat sinks connected in parallel and in series are carried out under different mass fluxes. In addition, a high-speed camera is used to observe flow patterns in the microchannels. The cold plate wall temperature (Tw), heat transfer coefficient (HTC), and pressure drop (PD) are obtained with the use of two microchannel heat sinks. The flow patterns of the bubbly flow and elongated bubbles in the microchannels are observed. The results of the analysis indicated that the Tw, HTC, and PD of the two microchannel heat sinks connected in parallel were degraded, especially when using the Type A-B parallel connection. Compared to the use of a single heat sink, the maximum decrease in HTC was 9.44 kW/(m2K) for Type A heat sinks connected in parallel, which represents a decrease of 45.95%. The influence of the series connection on the Tw, HTC, and PD of the two heat sinks is obvious. The Type A-A series connection exerted the greatest positive effect on the performance of the two heat sinks, especially in the case of the postposition heat sink. The maximum increase in HTC was 12.77 kW/(m2K) for the postposition Type A heat sink, representing an increase of 72.88%. These results could provide a reference for a two-phase flow-cooling complex for multiple heat sources with high heat flux.
Aetiology of Community-Acquired Pneumonia and the Role of Genetic Host Factors in Hospitalized Patients in Cyprus
Authors: Ladas Petros , Porfyridis Ilias , Tryfonos Christina , Ioannou Anna , Adamide Tonia , Christodoulou Christina , Richter Jan
Published: August 2023

Community-acquired pneumonia (CAP) remains the leading cause of hospitalization among infectious disease in Europe, and a major cause of morbidity and mortality. In order to determine and characterize the aetiology of CAP in hospitalized adults in Cyprus, respiratory and blood samples were obtained from hospitalized patients with CAP, and analyzed using Multiplex Real-Time PCR/RT-PCR, and ID/AMR enrichment panel (RPIP) analysis. Probe-based allelic discrimination was used to investigate genetic host factors in patients. The aetiology could be established in 87% of patients. The most prevalent viral pathogens detected were influenza A, SARS-CoV-2, and human rhinovirus. The most common bacterial pathogens detected were Streptococcus pneumoniae, Staphylococcus aureus, and Haemophilus influenzae. Antimicrobial resistance genes were identified in 23 patients. S. aureus was the most common AMR correlated strain in our study. A positive correlation was detected between bacterial infections and the NOS3 rs1799983 G allele and the FCGR2A rs1801274 G allele. A positive correlation was also detected between the TNF-α rs1800629 A allele and sepsis, while a negative correlation was detected with the ACE rs1799752 insertion genotype and the severity of pneumonia. In conclusion, the targeted NGS panel approach applied provides highly sensitive, comprehensive pathogen detection, in combination with antimicrobial resistance AMR insights that can guide treatment choices. In addition, several host factors have been identified that impact the disease progression and outcome.
Microbiota Profiling on Veterinary Faculty Restroom Surfaces and Source Tracking
Authors: Jabri Hiba , Krings Simone , Fall Papa Abdoulaye , Baurain Denis , Daube Georges , Taminiau Bernard
Published: August 2023

In this study, we aimed to develop a comprehensive microbial source amplicon database tailored for source tracking in veterinary settings. We rigorously tested our locally curated source tracking database by selecting a frequently accessed environment by veterinary students and veterinarians. By exploring the composition of resident microbiota and identifying potential sources of contamination, including animals, the environment, and human beings, we aimed to provide valuable insights into the dynamics of microbial transmission within veterinary facilities. The 16S rDNA amplicon sequencing was used to determine the bacterial taxonomic profiles of restroom surfaces. Bacterial sources were identified by linking our metadata-enriched local database to the microbiota profiling analysis using high-quality sequences. Microbiota profiling shows the dominance of four phyla: Actinobacteria, Bacteroidetes, Proteobacteria, and Firmicutes. If the restroom cleaning process did not appear to impact microbiota composition, significant differences regarding bacterial distribution were observed between male and female users in different sampling campaigns. Combining 16S rDNA profiling to our specific sources labeling pipeline, we found aquatic and human sources were the primary environment keywords in our campaigns. The probable presence of known animal sources (bovids, insects, equids, suids…) associated with bacterial genera such as Chryseobacterium, Bergeyella, Fibrobacter, and Syntrophococcus was also involved in restroom surfaces, emphasizing the proximity between these restrooms and the exchange of bacteria between people involved in animals handling. To summarize, we have demonstrated that DNA sequence-based source tracking may be integrated with high-throughput bacterial community analysis to enrich microbial investigation of potential bacterial contamination sources, especially for little known or poorly identified taxa. However, more research is needed to determine the tool’s utility in other applications.
Immunomodulatory Gene-Splicing Dysregulation in Tumorigenesis: Unmasking the Complexity
Authors: Maebele Lorraine Tshegofatso , Mulaudzi Thanyani Victor , Yasasve Madhavan , Dlamini Zodwa , Damane Botle Precious
Published: August 2023

Cancer is a global health concern with rising incidence, morbidity, and mortality. The interaction between the tumor and immune cells within the tumor microenvironment is facilitated by signaling pathways driven by immunomodulatory proteins. Alternative splicing regulates the production of multiple immunomodulatory proteins with diverse functionality from a single mRNA transcript. Splicing factors are pivotal in modulating alternative splicing processes but are also subject to regulation. The dysregulation of alternative splicing may result from splicing factor (SF) abnormal expression levels and mutations in the cis and trans-acting elements and small nuclear RNA (snRNA) molecules. Aberrant splicing may generate abnormal mRNA transcripts encoding isoforms with altered functions that contribute to tumorigenesis or cancer progression. This review uncovers the complexity of immunomodulatory genes splicing dysregulation in oncogenesis. Identifying specific immunomodulatory splicing isoforms that contribute to cancer could be utilized to improve current immunotherapeutic drugs or develop novel therapeutic interventions for cancer.
Detection Method and Common Characteristics of Waste Solvent from Semiconductor Industry
Authors: Ni Jinjuan , Zhang Qing , Zhang Xianglin , Sun Zhilong , Bao Dali
Published: August 2023

The recycling of organic solvents is a widely discussed topic. The waste organic solvents from thin-film-transistor liquid-crystal display (TFT-LCD) production is characterized by large quantities, multiple types, and complex compositions. Thus, the unified and compatible component analysis method is important for studying the recovery of waste organic solvents. In our work, based on the study of existing analytical methods, we designed a compatible method for the analysis of moisture using Karl Fischer analysis, for the analysis of organic compounds using gas chromatography, and for the analysis of the photoresist and other solids by evaporation. These were specific methods for analyzing the components of near-total formulation thin-film-transistor liquid-crystal display waste organic solvent. The organic matter content was analyzed via gas chromatography with a CP-Sil8CB column and flame ionization detector. The initial temperature of the column was 90 °C and the holding time was 1 min. The heating rate was 30 °C/min. The temperature was raised to 270 °C for 7 min. The internal standard method and the external standard method were used to determine the content of the main components of organic compounds. The relative standard deviation of the analytical results was 1.14~2.93%, 1.21~4.74% and 0.61%, respectively. The analytical results had good accuracy, but the external standard method was better; the recoveries were 99.76~107.60%, 95.86~107.70%, and 95.23~96.88%, respectively. Based on the composition analysis, the composition rule of the waste organic solvent was summarized. Through the exploration of the effect of the waste solvent, the common characteristics of the waste solvent were obtained. This study provides a good strategy and an optimized method for improving the efficiency of organic solvent recovery.