bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Sonic Hedgehog in Craniofacial Patterning, Morphogenesis and Cranial Neural Crest Survival
Authors: Dworkin Sebastian , Boglev Yeliz , Owens Harley , Goldie Stephen
Published: August 2016

Craniofacial defects (CFD) are a significant healthcare problem worldwide. Understanding both the morphogenetic movements which underpin normal facial development, as well as the molecular factors which regulate these processes, forms the cornerstone of future diagnostic, and ultimately, preventative therapies. The soluble morphogen Sonic hedgehog (Shh), a vertebrate orthologue of Drosophila hedgehog, is a key signalling factor in the regulation of craniofacial skeleton development in vertebrates, operating within numerous tissue types in the craniofacial primordia to spatiotemporally regulate the formation of the face and jaws. This review will provide an overview of normal craniofacial skeleton development, and focus specifically on the known roles of Shh in regulating the development and progression of the first pharyngeal arch, which in turn gives rise to both the upper jaw (maxilla) and lower jaw (mandible).
Compatibilized Immiscible Polymer Blends for Gas Separations
Authors: Panapitiya Nimanka , Wijenayake Sumudu , Nguyen Do , Karunaweera Chamaal , Huang Yu , Balkus Kenneth , Musselman Inga , Ferraris John
Published: August 2016

Membrane-based gas separation has attracted a great deal of attention recently due to the requirement for high purity gasses in industrial applications like fuel cells, and because of environment concerns, such as global warming. The current methods of cryogenic distillation and pressure swing adsorption are energy intensive and costly. Therefore, polymer membranes have emerged as a less energy intensive and cost effective candidate to separate gas mixtures. However, the use of polymeric membranes has a drawback known as the permeability-selectivity tradeoff. Many approaches have been used to overcome this limitation including the use of polymer blends. Polymer blending technology synergistically combines the favorable properties of different polymers like high gas permeability and high selectivity, which are difficult to attain with a single polymer. During polymer mixing, polymers tend to uncontrollably phase separate due to unfavorable thermodynamics, which limits the number of completely miscible polymer combinations for gas separations. Therefore, compatibilizers are used to control the phase separation and to obtain stable membrane morphologies, while improving the mechanical properties. In this review, we focus on immiscible polymer blends and the use of compatibilizers for gas separation applications.
Impact of Glucocorticoid Excess on Glucose Tolerance: Clinical and Preclinical Evidence
Authors: Pasieka Aoibhe , Rafacho Alex
Published: August 2016

Glucocorticoids (GCs) are steroid hormones that exert important physiological actions on metabolism. Given that GCs also exert potent immunosuppressive and anti-inflammatory actions, synthetic GCs such as prednisolone and dexamethasone were developed for the treatment of autoimmune- and inflammatory-related diseases. The synthetic GCs are undoubtedly efficient in terms of their therapeutic effects, but are accompanied by significant adverse effects on metabolism, specifically glucose metabolism. Glucose intolerance and reductions in insulin sensitivity are among the major concerns related to GC metabolic side effects, which may ultimately progress to type 2 diabetes mellitus. A number of pre-clinical and clinical studies have aimed to understand the repercussions of GCs on glucose metabolism and the possible mechanisms of GC action. This review intends to summarize the main alterations that occur in liver, skeletal muscle, adipose tissue, and pancreatic islets in the context of GC-induced glucose intolerance. For this, both experimental (animals) and clinical studies were selected and, whenever possible, the main cellular mechanisms involved in such GC-side effects were discussed.
Potential Application of p-Coumaric Acid on Differentiation of C2C12 Skeletal Muscle and 3T3-L1 Preadipocytes—An in Vitro and in Silico Approach
Authors: Ilavenil Soundharrajan , Kim Da , Srigopalram Srisesharam , Arasu Mariadhas , Lee Kyung , Lee Jeong , Lee Jong , Renganathan Senthil , Choi Ki
Published: August 2016

Coumaric acid (CA) is a phenolic acid of the hydroxycinnamic acid family, and it has many biological functions such as anti-oxidant, anti-inflammatory, antidiabetic, anti-ulcer, anti-platelet, anti-cancer activities, etc. In the present study, we planned to analyse the potential molecular function of CA on skeletal muscle and preadipocytes differentiation using PCR and Western blot techniques. First, we analysed the impact of CA on C2C12 skeletal muscle differentiation. It revealed that CA treatment inhibited horse serum-induced skeletal muscle differentiation as evidenced by the decreased expression of early myogenic differentiation markers such as Myogenin and myoD via the AMP activated protein kinase- alpha AMPK-α mediated pathway. Furthermore, the level of lipid accumulation and changes in genes and protein expressions that are associated with lipogenesis and lipolysis were analyzed in 3T3-L1 cells. The Oil Red O staining evidenced that CA treatment inhibited lipid accumulation at the concentration of 0.1 and 0.2 mM. Furthermore, coumaric acid treatment decreased the expression of main transcriptional factors such as CCAAT/enhancer binding protein-alpha (C/EBP-α) and peroxisome proliferator-activated receptor gamma-2 (PPAR-γ2). Subsequently, CA treatment decreased the expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthase (FAS), acetyl CoA carboxylase (ACC) and adiponectin. Finally, we identified conformational changes induced by CA in PPAR-γ2 using computational biology tools. It revealed that CA might downregulate the PPAR-γ2 expression by directly binding with amino acids of PPAR-γ2 by hydrogen at 3.26 distance and hydrophobic interactions at 3.90 contact distances. These data indicated that CA suppressed skeletal muscle and preadipocytes differentiation through downregulation of the main transcriptional factors and their downstream targets.
The in Vitro and in Vivo Antitumor Activities of Tetracyclic Triterpenoids Compounds Actein and 26-Deoxyactein Isolated from Rhizome of Cimicifuga foetida L.
Authors: Wu Desong , Yao Qi , Chen Yajuan , Hu Xiaodong , Qing Chen , Qiu Minghua
Published: August 2016

Aims: This work aims to study the in vitro and in vivo antitumor activities of tetracyclic triterpenoids compounds actein and 26-deoxyactein. Further, the mechanism is investigated. Methods: In vitro, a modified MTT method was used to assay the cytotoxicities of actein and 26-deoxyactein in 12 human tumor cell lines. In vivo, mouse sarcoma S180 and human lung cancer A549 cells were respectively implanted subcutaneously in ICR mice and nude mice to establish implanted tumor models. Flow cytometry (FCM) was used to assay the cycle distribution of the tumor cells. Immunohistochemistry was used to measure CD31-positive expression in the xenogrft tumor by analyzing microvessel density (MVD). In addition, acute toxicities of actein and 26-deoxyactein were also evaluated. Results: Actein and 26-deoxyactein inhibited the proliferation of the 12 human cancer cell lines tested with the values of 50% inhibitory concentrations (IC50) between 12.29 and 88.39 μg/mL. In vivo, both actein (3–27 mg/kg) and 26-deoxyactein (3–27 mg/kg) significantly inhibited the growth of the implanted sarcoma S180 in a dose-dependent manner. Actein (10, 30 mg/kg) and 26-deoxyactein (10, 30 mg/kg) markedly inhibited the xenograft growth with T/C (%) values of 38%, 55% for actein, and 35%, 49% for 26-deoxyactein. Compared with the vehicle control, actein (10, 30 mg/kg) and 26-deoxyactein (10, 30 mg/kg) significantly reduced the MVD in the xenograft tumor. The FCM result showed that human leukemia HL-60 cells were arrested at G1 phase after treated with either actein (6.25–25 μg/mL) or 26-deoxyactein (6.25–25 μg/mL) for 48 h. A limited trial in mice showed that both of the minimal lethal doses (MLDs) of actein and 26-deoxyactein were over 5 g/kg. Conclusions: Both actein and 26-deoxyactein have low toxicities. Importantly, both these two tetracyclic triterpenoids compounds isolated from rhizome of Cimicifuga foetida L. have significant antitumor activities in vitro and in vivo, which is associated with cell cycle arrest and angiogenesis inhibition.
Stimuli-Responsive Polymeric Systems for Controlled Protein and Peptide Delivery: Future Implications for Ocular Delivery
Authors: Mahlumba Pakama , Choonara Yahya , Kumar Pradeep , Du Toit Lisa , Pillay Viness
Published: August 2016

Therapeutic proteins and peptides have become notable in the drug delivery arena for their compatibility with the human body as well as their high potency. However, their biocompatibility and high potency does not negate the existence of challenges resulting from physicochemical properties of proteins and peptides, including large size, short half-life, capability to provoke immune responses and susceptibility to degradation. Various delivery routes and delivery systems have been utilized to improve bioavailability, patient acceptability and reduce biodegradation. The ocular route remains of great interest, particularly for responsive delivery of macromolecules due to the anatomy and physiology of the eye that makes it a sensitive and complex environment. Research in this field is slowly gaining attention as this could be the breakthrough in ocular drug delivery of macromolecules. This work reviews stimuli-responsive polymeric delivery systems, their use in the delivery of therapeutic proteins and peptides as well as examples of proteins and peptides used in the treatment of ocular disorders. Stimuli reviewed include pH, temperature, enzymes, light, ultrasound and magnetic field. In addition, it discusses the current progress in responsive ocular drug delivery. Furthermore, it explores future prospects in the use of stimuli-responsive polymers for ocular delivery of proteins and peptides. Stimuli-responsive polymers offer great potential in improving the delivery of ocular therapeutics, therefore there is a need to consider them in order to guarantee a local, sustained and ideal delivery of ocular proteins and peptides, evading tissue invasion and systemic side-effects.
Novel Combination of Prebiotics Galacto-Oligosaccharides and Inulin-Inhibited Aberrant Crypt Foci Formation and Biomarkers of Colon Cancer in Wistar Rats
Authors: Qamar Tahir , Syed Fatima , Nasir Muhammad , Rehman Habib , Zahid Muhammad , Liu Rui , Iqbal Sanaullah
Published: August 2016

The selectivity and beneficial effects of prebiotics are mainly dependent on composition and glycosidic linkage among monosaccharide units. This is the first study to use prebiotic galacto-oligosaccharides (GOS) that contains β-1,6 and β-1,3 glycosidic linkages and the novel combination of GOS and inulin in cancer prevention. The objective of the present study is to explore the role of novel GOS and inulin against various biomarkers of colorectal cancer (CRC) and the incidence of aberrant crypt foci (ACF) in a 1,2-dimethyl hydrazine dihydrochloride (DMH)-induced rodent model. Prebiotic treatments of combined GOS and inulin (57 mg each), as well as individual doses (GOS: 76–151 mg; inulin 114 mg), were given to DMH-treated animals for 16 weeks. Our data reveal the significant preventive effect of the GOS and inulin combination against the development of CRC. It was observed that inhibition of ACF formation (55.8%) was significantly (p ≤ 0.05) higher using the GOS and inulin combination than GOS (41.4%) and inulin (51.2%) treatments alone. This combination also rendered better results on short-chain fatty acids (SCFA) and bacterial enzymatic activities. Dose-dependent effects of prebiotic treatments were also observed on cecum and fecal bacterial enzymes and on SCFA. Thus, this study demonstrated that novel combination of GOS and inulin exhibited stronger preventive activity than their individual treatments alone, and can be a promising strategy for CRC chemoprevention.
Usual Intake of Key Minerals among Children in the Second Year of Life, NHANES 2003–2012
Authors: Hamner Heather , Perrine Cria , Scanlon Kelley
Published: August 2016

Iron, calcium, and zinc are important nutrients for the young, developing child. This study describes the usual intake of iron, calcium, and zinc among US children in the second year of life using two days of dietary intake data from the National Health and Nutrition Examination Survey 2003–2012. Estimates were calculated using PC-SIDE to account for within and between person variation. Mean usual iron, calcium, and zinc intakes were 9.5 mg/day, 1046 mg/day, and 7.1 mg/day, respectively. Over a quarter of children had usual iron intakes less than the Recommended Dietary Allowance (RDA) (26.1%). Eleven percent of children had usual calcium intakes below the RDA and over half of children had usual intakes of zinc that exceeded the tolerable upper intake level (UL). Two percent or less had usual intakes below the Estimated Average Requirement (EAR) for iron, calcium, and zinc. Our findings suggest that during 2003–2012, one in four children and one in ten children had usual intakes below the RDA for iron and calcium, respectively. Children who are not meeting their nutrient requirements could be at increased risk for developing deficiencies such as iron deficiency or could lead to a shortage in adequate nutrients required for growth and development. One in every two children is exceeding the UL for zinc, but the interpretation of these estimates should be done with caution given the limited data on adverse health outcomes. Continued monitoring of zinc intake and further assessment for the potential of adverse health outcomes associated with high zinc intakes may be needed.
Protein-Pacing Caloric-Restriction Enhances Body Composition Similarly in Obese Men and Women during Weight Loss and Sustains Efficacy during Long-Term Weight Maintenance
Authors: Arciero Paul , Edmonds Rohan , He Feng , Ward Emery , Gumpricht Eric , Mohr Alex , Ormsbee Michael , Astrup Arne
Published: August 2016

Short-Term protein-pacing (P; ~6 meals/day, >30% protein/day) and caloric restriction (CR, ~25% energy deficit) improves total (TBF), abdominal (ABF) and visceral (VAT) fat loss, energy expenditure, and biomarkers compared to heart healthy (HH) recommendations (3 meals/day, 15% protein/day) in obese adults. Less is known whether obese men and women respond similarly to P-CR during weight loss (WL) and whether a modified P-CR (mP-CR) is more efficacious than a HH diet during long-term (52 week) weight maintenance (WM). The purposes of this study were to evaluate the efficacy of: (1) P-CR on TBF, ABF, resting metabolic rate (RMR), and biomarkers between obese men and women during WL (weeks 0–12); and (2) mP-CR compared to a HH diet during WM (weeks 13–64). During WL, men (n = 21) and women (n = 19) were assessed for TBF, ABF, VAT, RMR, and biomarkers at weeks 0 (pre) and 12 (post). Men and women had similar reductions (p < 0.01) in weight (10%), TBF (19%), ABF (25%), VAT (33%), glucose (7%–12%), insulin (40%), leptin (>50%) and increase in % lean body mass (9%). RMR (kcals/kg bodyweight) was unchanged and respiratory quotient decreased 9%. Twenty-four subjects (mP-CR, n = 10; HH, n = 14) completed WM. mP-CR regained significantly less body weight (6%), TBF (12%), and ABF (17%) compared to HH (p < 0.05). Our results demonstrate P-CR enhances weight loss, body composition and biomarkers, and maintains these changes for 52-weeks compared to a traditional HH diet.
Frequency Tuning and Modulation of a Quantum Cascade Laser with an Integrated Resistive Heater
Authors: Gürel Kutan , Schilt Stéphane , Bismuto Alfredo , Bidaux Yves , Tardy Camille , Blaser Stéphane , Gresch Tobias , Südmeyer Thomas
Published: August 2016

We present a detailed experimental investigation of the use of a novel actuator for frequency tuning and modulation in a quantum cascade laser (QCL) based on a resistive integrated heater (IH) placed close to the active region. This new actuator is attractive for molecular spectroscopy applications as it enables fast tuning of the QCL wavelength with a minor influence on the optical output power, and is electrically-controlled. Using a spectroscopic setup comprising a low-pressure gas cell, we measured the tuning and modulation properties of a QCL emitting at 7.8 μm as a function of the active region and IH currents. We show that a current step applied to the IH enables the laser frequency to be switched by 500 MHz in a few milliseconds, as fast as for a step of the current in the active region, and limited by heat dissipation towards the laser sub-mount. The QCL optical frequency can be modulated up to ~100 kHz with the IH current, which is one order of magnitude slower than for the QCL current, but sufficient for many spectroscopic applications. We discuss the experimental results using a thermal model of the heat transfer in terms of cascaded low-pass filters and extract the respective cut-off frequencies. Finally, we present a proof-of-principle experiment of wavelength modulation spectroscopy of a N2O transition performed with a modulation of the IH current and show some potential benefits in comparison to QCL current modulation, which results from the reduced associated amplitude modulation.
Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences
Authors: Bravo-Anaya Lourdes , Pignon Frédéric , Martínez Félix , Rinaudo Marguerite
Published: August 2016

Molecular weight, stiffness, temperature, and polymer and ionic concentrations are known to widely influence the viscosity of polymer solutions. Additionally, polymer molecular weight—which is related to its dimensions in solution—is one of its most important characteristics. In this communication, low molecular weight DNA from salmon sperm was purified and then studied in solutions in a wide concentration range (between 0.5 and 1600 mg/mL). The intrinsic viscosity of this low molecular weight DNA sample was firstly determined and the evidence of the overlap concentration was detected around the concentration of 125 mg/mL. The chain characteristics of these short molecules were studied in terms of the influence of their molecular weight on the solution viscosities and on the overlap parameter CDNA[η]. Furthermore, to complete previously reported experimental data, solutions of a large molecular weight DNA from calf-thymus were studied in a high concentration range (up to 40 mg/mL). The rheological behavior is discussed in terms of the generalized master curve obtained from the variation of the specific viscosity at zero shear rate (ηsp,0) as a function of CDNA[η].
On-Ground Processing of Yaogan-24 Remote Sensing Satellite Attitude Data and Verification Using Geometric Field Calibration
Authors: Wang Mi , Fan Chengcheng , Yang Bo , Jin Shuying , Pan Jun
Published: August 2016

Satellite attitude accuracy is an important factor affecting the geometric processing accuracy of high-resolution optical satellite imagery. To address the problem whereby the accuracy of the Yaogan-24 remote sensing satellite’s on-board attitude data processing is not high enough and thus cannot meet its image geometry processing requirements, we developed an approach involving on-ground attitude data processing and digital orthophoto (DOM) and the digital elevation model (DEM) verification of a geometric calibration field. The approach focuses on three modules: on-ground processing based on bidirectional filter, overall weighted smoothing and fitting, and evaluation in the geometric calibration field. Our experimental results demonstrate that the proposed on-ground processing method is both robust and feasible, which ensures the reliability of the observation data quality, convergence and stability of the parameter estimation model. In addition, both the Euler angle and quaternion could be used to build a mathematical fitting model, while the orthogonal polynomial fitting model is more suitable for modeling the attitude parameter. Furthermore, compared to the image geometric processing results based on on-board attitude data, the image uncontrolled and relative geometric positioning result accuracy can be increased by about 50%.
DeepFruits: A Fruit Detection System Using Deep Neural Networks
Authors: Sa Inkyu , Ge Zongyuan , Dayoub Feras , Upcroft Ben , Perez Tristan , McCool Chris
Published: August 2016

This paper presents a novel approach to fruit detection using deep convolutional neural networks. The aim is to build an accurate, fast and reliable fruit detection system, which is a vital element of an autonomous agricultural robotic platform; it is a key element for fruit yield estimation and automated harvesting. Recent work in deep neural networks has led to the development of a state-of-the-art object detector termed Faster Region-based CNN (Faster R-CNN). We adapt this model, through transfer learning, for the task of fruit detection using imagery obtained from two modalities: colour (RGB) and Near-Infrared (NIR). Early and late fusion methods are explored for combining the multi-modal (RGB and NIR) information. This leads to a novel multi-modal Faster R-CNN model, which achieves state-of-the-art results compared to prior work with the F1 score, which takes into account both precision and recall performances improving from 0 . 807 to 0 . 838 for the detection of sweet pepper. In addition to improved accuracy, this approach is also much quicker to deploy for new fruits, as it requires bounding box annotation rather than pixel-level annotation (annotating bounding boxes is approximately an order of magnitude quicker to perform). The model is retrained to perform the detection of seven fruits, with the entire process taking four hours to annotate and train the new model per fruit.
Research on the Participant Behavior Selections of the Energy Performance Contracting Project Based on the Robustness of the Shared Savings Contract
Authors: Xing Guangyuan , Qian Dong , Guo Ju’e
Published: August 2016

The profits of the ESCO (Energy Services Company) and EU (Energy Using Organization) in the EPCP (energy performance contracting project) rely on the signing of the shared savings contract and the successful operation of the project, and the probability of the project’s success is decided by the complementary efforts of the ESCO and EU. However, the effort selection of the two sides face the bidirectional moral hazard caused by asymmetric information. Based on the robustness of shared savings contract, this paper establishes a bidirectional moral hazard model under asymmetric information to analyze the complementary efforts selection of the ESCO and EU with the given revenue sharing rules, and analyzes the differences of the complementary efforts under symmetric and asymmetric information conditions and the impacts of those efforts on the shared savings contract’s robustness by using a numerial simulation. The results show that compared with information symmetry, the bidirectional moral hazard will erode the project’s value under information asymmetry, the project’s success probability and the level of the parties’ efforts will decrease, which reveals the negative impact of asymmetric information on the robustness of the shared savings contract, and the significance of eliminating information asymmetry effectively as well as incentivizing the parties to increase the degree of complementary efforts to enhance the probability of the project’s success. Finally, policy recommendations regarding the introduction of incomplete contracts, promoting guaranteed savings contracts, and improving energy savings audits for the enhancement of the robustness of the shared savings contract are provided. This research will be helpful to improve the theoretical research on the contract’s robustness, perfect the design of the energy service contract, and formulate the related support policies.
Rural–Urban Linkages and Sustainable Regional Development: The Role of Entrepreneurs in Linking Peripheries and Centers
Authors: Mayer Heike , Habersetzer Antoine , Meili Rahel
Published: August 2016

Urban and rural areas differ in economic, social and environmental terms. Due to the diverging dynamics in urban and rural areas, the social and economic distance between them might increase in the future even more. Rural entrepreneurs with linkages to urban areas are able to bridge the rural–urban divide by accessing some of the urban features, such as knowledge and markets, while at the same time profiting from the advantages of their peripheral location. This paper highlights exploratory results from qualitative interviews with rural entrepreneurs, and we illustrate entrepreneurial linkages to urban centers. The interview data show that rural entrepreneurs with rural–urban linkages develop sensibility for core market demands and trends, they valuate rural assets, and they combine rural and urban sources of knowledge for innovation. Through their entrepreneurial activity, rural entrepreneurs with linkages to urban areas might constitute an opposite force to polarizing concentration of economic activities in cities. Hence, rural entrepreneurs with urban linkages might contribute to sustainable economic relationships between urban and rural areas.
Selective Membrane Redistribution and Depletion of Gαq-Protein by Pasteurella multocida Toxin
Authors: Clemons Nathan , Luo Shuhong , Ho Mengfei , Wilson Brenda
Published: August 2016

Pasteurella multocida toxin (PMT), the major virulence factor responsible for zoonotic atrophic rhinitis, is a protein deamidase that activates the alpha subunit of heterotrimeric G proteins. Initial activation of G alpha-q-coupled phospholipase C-beta-1 signaling by PMT is followed by uncoupling of G alpha-q-dependent signaling, causing downregulation of downstream calcium and mitogenic signaling pathways. Here, we show that PMT decreases endogenous and exogenously expressed G alpha-q protein content in host cell plasma membranes and in detergent resistant membrane (DRM) fractions. This membrane depletion of G alpha-q protein was dependent upon the catalytic activity of PMT. Results indicate that PMT-modified G alpha-q redistributes within the host cell membrane from the DRM fraction into the soluble membrane and cytosolic fractions. In contrast, PMT had no affect on G alpha-s or G beta protein levels, which are not substrate targets of PMT. PMT also had no affect on G alpha-11 levels, even though G alpha-11 can serve as a substrate for deamidation by PMT, suggesting that membrane depletion of PMT-modified G-alpha-q has specificity.
Clomiphene and Its Isomers Block Ebola Virus Particle Entry and Infection with Similar Potency: Potential Therapeutic Implications
Authors: Nelson Elizabeth , Barnes Alyson , Wiehle Ronald , Fontenot Gregory , Hoenen Thomas , White Judith
Published: August 2016

The 2014 outbreak of Ebola virus (EBOV) in Western Africa highlighted the need for anti-EBOV therapeutics. Clomiphene is a U.S. Food and Drug Administration (FDA)-approved drug that blocks EBOV entry and infection in cells and significantly protects EBOV-challenged mice. As provided, clomiphene is, approximately, a 60:40 mixture of two stereoisomers, enclomiphene and zuclomiphene. The pharmacokinetic properties of the two isomers vary, but both accumulate in the eye and male reproductive tract, tissues in which EBOV can persist. Here we compared the ability of clomiphene and its isomers to inhibit EBOV using viral-like particle (VLP) entry and transcription/replication-competent VLP (trVLP) assays. Clomiphene and its isomers inhibited the entry and infection of VLPs and trVLPs with similar potencies. This was demonstrated with VLPs bearing the glycoproteins from three filoviruses (EBOV Mayinga, EBOV Makona, and Marburg virus) and in two cell lines (293T/17 and Vero E6). Visual problems have been noted in EBOV survivors, and viral RNA has been isolated from semen up to nine months post-infection. Since the clomiphene isomers accumulate in these affected tissues, clomiphene or one of its isomers warrants consideration as an anti-EBOV agent, for example, to potentially help ameliorate symptoms in EBOV survivors.
Measles Virus Hemagglutinin Protein Epitopes: The Basis of Antigenic Stability
Authors: Tahara Maino , Bürckert Jean-Philippe , Kanou Kazuhiko , Maenaka Katsumi , Muller Claude , Takeda Makoto
Published: August 2016

Globally eliminating measles using available vaccines is biologically feasible because the measles virus (MV) hemagglutinin (H) protein is antigenically stable. The H protein is responsible for receptor binding, and is the main target of neutralizing antibodies. The immunodominant epitope, known as the hemagglutinating and noose epitope, is located near the receptor-binding site (RBS). The RBS also contains an immunodominant epitope. Loss of receptor binding correlates with an escape from the neutralization by antibodies that target the epitope at RBS. Another neutralizing epitope is located near RBS and is shielded by an N-linked sugar in certain genotype strains. However, human sera from vaccinees and measles patients neutralized all MV strains with similar efficiencies, regardless of the N-linked sugar modification or mutations at these epitopes. Two other major epitopes exist at a distance from RBS. One has an unstructured flexible domain with a linear neutralizing epitope. When MV-H forms a tetramer (dimer of dimers), these epitopes may form the dimer-dimer interface, and one of the two epitopes may also interact with the F protein. The neutralization mechanisms of antibodies that recognize these epitopes may involve inhibiting the H-F interaction or blocking the fusion cascade after MV-H binds to its receptors.
The Use of TRMM 3B42 Product for Drought Monitoring in Mexico
Authors: De Jesús Aurea , Breña-Naranjo Jose , Pedrozo-Acuña Adrián , Alcocer Yamanaka Victor
Published: August 2016

Drought has been a recurrent phenomenon in Mexico. For its assessment and monitoring, several studies have monitored meteorological droughts using standardized indices of precipitation deficits. Such conventional studies have mostly relied on rain gauge-based measurements, with the main limitation being the scarcity of rain gauge spatial coverage. This issue does not allow a robust spatial characterization of drought. A recent alternative for monitoring purposes can be found in satellite-based remote sensing of meteorological variables. The main objective of this study is to evaluate the standardized precipitation index (SPI) in Mexico during the period 1998 to 2013, using the Tropical Rainfall Measuring Mission (TRMM) satellite product 3B42. Results suggest that Mexico experienced the driest conditions during the great drought between 2011 and 2012; however, temporal variability in the SPI was found across different climatic regions. Nevertheless, a comparison of the SPI derived by TRMM against the rain gauge-based SPI computed by the official Mexican Drought Monitor showed low to medium correlation of the time series though both SPIs managed to capture the most relevant droughts at the national scale. We conclude that the TRMM product can properly monitor meteorological droughts despite its relative short dataset length (~15 years). Finally, we recommend an assimilation of rain gauge and satellite-based precipitation data to provide more robust estimates of meteorological drought severity.
EXAMINING TATOOINE: ATMOSPHERIC MODELS OF NEPTUNE-LIKE CIRCUMBINARY PLANETS
Authors: May E. M. , Rauscher E.
Published: August 2016

Circumbinary planets experience a time-varying irradiation pattern as they orbit their two host stars. In this work, we present the first detailed study of the atmospheric effects of this irradiation pattern on known and hypothetical gaseous circumbinary planets. Using both a one-dimensional energy balance model (EBM) and a three-dimensional general circulation model (GCM), we look at the temperature differences between circumbinary planets and their equivalent single-star cases in order to determine the nature of the atmospheres of these planets. We find that for circumbinary planets on stable orbits around their host stars, temperature differences are on average no more than 1.0% in the most extreme cases. Based on detailed modeling with the GCM, we find that these temperature differences are not large enough to excite circulation differences between the two cases. We conclude that gaseous circumbinary planets can be treated as their equivalent single-star case in future atmospheric modeling efforts.
TRANSIENT GALACTIC COSMIC-RAY MODULATION DURING SOLAR CYCLE 24: A COMPARATIVE STUDY OF TWO PROMINENT FORBUSH DECREASE EVENTS
Authors: Zhao L.-L. , Zhang H.
Published: August 2016

Forbush decrease (FD) events are of great interest for transient galactic cosmic-ray (GCR) modulation study. In this study, we perform comparative analysis of two prominent Forbush events during cycle 24, occurring on 2012 March 8 (Event 1) and 2015 June 22 (Event 2), utilizing the measurements from the worldwide neutron monitor (NM) network. Despite their comparable magnitudes, the two Forbush events are distinctly different in terms of evolving GCR energy spectrum and energy dependence of the recovery time. The recovery time of Event 1 is strongly dependent on the median energy, compared to the nearly constant recovery time of Event 2 over the studied energy range. Additionally, while the evolutions of the energy spectra during the two FD events exhibit similar variation patterns, the spectrum of Event 2 is significantly harder, especially at the time of deepest depression. These difference are essentially related to their associated solar wind disturbances. Event 1 is associated with a complicated shock-associated interplanetary coronal mass ejection (ICME) disturbance with large radial extent, probably formed by the merging of multiple shocks and transient flows, and which delivered a glancing blow to Earth. Conversely, Event 2 is accompanied by a relatively simple halo ICME with small radial extent that hit Earth more head-on.
ABSOLUTE INTEGRAL CROSS SECTIONS FOR THE STATE-SELECTED ION–MOLECULE REACTION
; v+ = 0–2) + C2H2 IN THE COLLISION ENERGY RANGE OF 0.03–10.00 eV
Authors: Xu Yuntao , Xiong Bo , Chang Yih Chung , Ng C. Y.
Published: August 2016

Using the vacuum ultraviolet laser pulsed field ionization-photoion source, together with the double-quadrupole–double-octopole mass spectrometer developed in our laboratory, we have investigated the state-selected ion–molecule reaction

; v
+ = 0–2, N+ = 0–9) + C2H2, achieving high internal-state selectivity and high kinetic energy resolution for reactant

ions. The charge transfer (CT) and hydrogen-atom transfer (HT) channels, which lead to the respective formation of product

and N2H+ ions, are observed. The vibrationally selected absolute integral cross sections for the CT [σ
CT(v
+)] and HT [[σ
HT(v
+)] channels obtained in the center-of-mass collision energy (E
cm) range of 0.03–10.00 eV reveal opposite E
cm dependences. The σ
CT(v
+) is found to increase as E
cm is decreased, and is consistent with the long-range exothermic CT mechanism, whereas the E
cm enhancement observed for the σ
HT(v
+) suggests effective coupling of kinetic energy to internal energy, enhancing the formation of N2H+. The σ
HT(v
+) curve exhibits a step at E
cm = 0.70–1.00 eV, suggesting the involvement of the excited

state in the HT reaction. Contrary to the strong E
cm dependences for σ
CT(v
+) and σ
HT(v
+), the effect of vibrational excitation of

on both the CT and HT channels is marginal. The branching ratios and cross sections for the CT and HT channels determined in the present study are useful for modeling the atmospheric compositions of Saturn's largest moon, Titan. These cross sections and branching ratios are also valuable for benchmarking theoretical calculations on chemical dynamics of the titled reaction.
HST HOT-JUPITER TRANSMISSION SPECTRAL SURVEY: CLEAR SKIES FOR COOL SATURN WASP-39b
Authors: Fischer Patrick D. , Knutson Heather A. , Sing David K. , Henry Gregory W. , Williamson Michael W. , Fortney Jonathan J. , Burrows Adam S. , Kataria Tiffany , Nikolov Nikolay , Showman Adam P. , Ballester Gilda E. , Désert Jean-Michel , Aigrain Suzanne , Deming Drake , Etangs Alain Lecavelier des , Vidal-Madjar Alfred
Published: August 2016

We present the Hubble Space Telescope (HST) Space Telescope Imaging Spectrograph (STIS) optical transmission spectroscopy of the cool Saturn-mass exoplanet WASP-39b from 0.29-1.025 μm, along with complementary transit observations from Spitzer IRAC at 3.6 and 4.5 μm. The low density and large atmospheric pressure scale height of WASP-39b make it particularly amenable to atmospheric characterization using this technique. We detect a Rayleigh scattering slope as well as sodium and potassium absorption features; this is the first exoplanet in which both alkali features are clearly detected with the extended wings predicted by cloud-free atmosphere models. The full transmission spectrum is well matched by a clear H2-dominated atmosphere, or one containing a weak contribution from haze, in good agreement with the preliminary reduction of these data presented in Sing et al.  WASP-39b is predicted to have a pressure-temperature profile comparable to that of HD 189733b and WASP-6b, making it one of the coolest transiting gas giants observed in our HST STIS survey. Despite this similarity, WASP-39b appears to be largely cloud-free, while the transmission spectra of HD 189733b and WASP-6b both indicate the presence of high altitude clouds or hazes. These observations further emphasize the surprising diversity of cloudy and cloud-free gas giant planets in short-period orbits and the corresponding challenges associated with developing predictive cloud models for these atmospheres.
CSO AND CARMA OBSERVATIONS OF L1157. II. CHEMICAL COMPLEXITY IN THE SHOCKED OUTFLOW
Authors: Burkhardt Andrew M. , Dollhopf Niklaus M. , Corby Joanna F. , Carroll P. Brandon , Shingledecker Christopher N. , Loomis Ryan A. , Booth Shawn Thomas , Blake Geoffrey A. , Herbst Eric , Remijan Anthony J. , McGuire Brett A.
Published: August 2016

L1157, a molecular dark cloud with an embedded Class 0 protostar possessing a bipolar outflow, is an excellent source for studying shock chemistry, including grain-surface chemistry prior to shocks, and post-shock, gas-phase processing. The L1157-B1 and B2 positions experienced shocks at an estimated ∼2000 and 4000 years ago, respectively. Prior to these shock events, temperatures were too low for most complex organic molecules to undergo thermal desorption. Thus, the shocks should have liberated these molecules from the ice grain-surfaces en masse, evidenced by prior observations of SiO and multiple grain mantle species commonly associated with shocks. Grain species, such as OCS, CH3OH, and HNCO, all peak at different positions relative to species that are preferably formed in higher-velocity shocks or repeatedly shocked material, such as SiO and HCN. Here, we present high spatial resolution (∼3″) maps of CH3OH, HNCO, HCN, and HCO+ in the southern portion of the outflow containing B1 and B2, as observed with Combined Array for Research in Millimeter-Wave Astronomy. The HNCO maps are the first interferometric observations of this species in L1157. The maps show distinct differences in the chemistry within the various shocked regions in L1157B. This is further supported through constraints of the molecular abundances using the non-LTE code radex. We find that the east/west chemical differentiation in C2 may be explained by the contrast of the shock’s interaction with either cold, pristine material or warm, previously shocked gas, as seen in enhanced HCN abundances. In addition, the enhancement of the HNCO abundance toward the the older shock, B2, suggests the importance of high-temperature O-chemistry in shocked regions.
HIGH-PRECISION RADIO AND INFRARED ASTROMETRY OF LSPM J1314+1320AB. I. PARALLAX, PROPER MOTIONS, AND LIMITS ON PLANETS
Authors: Forbrich Jan , Dupuy Trent J. , Reid Mark J. , Berger Edo , Rizzuto Aaron , Mann Andrew W. , Liu Michael C. , Aller Kimberly , Kraus Adam L.
Published: August 2016

We present multi-epoch astrometric radio observations with the Very Long Baseline Array (VLBA) of the young ultracool-dwarf binary LSPM J1314+1320AB. The radio emission comes from the secondary star. Combining the VLBA data with Keck near-infrared adaptive-optics observations of both components, a full astrometric fit of parallax (π
abs = 57.975 ± 0.045 mas, corresponding to a distance of d = 17.249 ± 0.013 pc), proper motion (μ

αcos δ
= −247.99 ± 0.10 mas yr−1, μ

δ
= −183.58 ± 0.22 mas yr−1), and orbital motion is obtained. Despite the fact that the two components have nearly identical masses to within ±2%, the secondary’s radio emission exceeds that of the primary by a factor of ≳30, suggesting a difference in stellar rotation history, which could result in different magnetic field configurations. Alternatively, the emission could be anisotropic and beamed toward us for the secondary but not for the primary. Using only reflex motion, we exclude planets of mass 0.7–10 M
jup with orbital periods of 600–10 days, respectively. Additionally, we use the full orbital solution of the binary to derive an upper limit for the semimajor axis of 0.23 au for stable planetary orbits within this system. These limits cover a parameter space that is inaccessible with, and complementary to, near-infrared radial velocity surveys of ultracool dwarfs. Our absolute astrometry will constitute an important test for the astrometric calibration of Gaia.
IMPLICATIONS OF THE COROTATION THEOREM ON THE MRI IN AXIAL SYMMETRY
Authors: Montani G. , Cianfrani F. , Pugliese D.
Published: August 2016

We analyze the linear stability of an axially symmetric ideal plasma disk, embedded in a magnetic field and endowed with a differential rotation. This study is performed by adopting the magnetic flux function as the fundamental dynamical variable, in order to outline the role played by the corotation theorem on the linear mode structure. Using some specific assumptions (e.g., plasma incompressibility and propagation of the perturbations along the background magnetic field), we select the Alfvénic nature of the magnetorotational instability, and, in the geometric optics limit, we determine the dispersion relation describing the linear spectrum. We show how the implementation of the corotation theorem (valid for the background configuration) on the linear dynamics produces the cancellation of the vertical derivative of the disk angular velocity (we check such a feature also in the standard vector formalism to facilitate comparison with previous literature, in both the axisymmetric and three-dimensional cases). As a result, we clarify that the unstable modes have, for a stratified disk, the same morphology, proper of a thin-disk profile, and the z-dependence has a simple parametric role.
A MACHINE-LEARNING APPROACH TO MEASURING THE ESCAPE OF IONIZING RADIATION FROM GALAXIES IN THE REIONIZATION EPOCH
Authors: Jensen Hannes , Zackrisson Erik , Pelckmans Kristiaan , Binggeli Christian , Ausmees Kristiina , Lundholm Ulrika
Published: August 2016

Recent observations of galaxies at

, along with the low value of the electron scattering optical depth measured by the Planck mission, make galaxies plausible as dominant sources of ionizing photons during the epoch of reionization. However, scenarios of galaxy-driven reionization hinge on the assumption that the average escape fraction of ionizing photons is significantly higher for galaxies in the reionization epoch than in the local universe. The NIRSpec instrument on the James Webb Space Telescope (JWST) will enable spectroscopic observations of large samples of reionization-epoch galaxies. While the leakage of ionizing photons will not be directly measurable from these spectra, the leakage is predicted to have an indirect effect on the spectral slope and the strength of nebular emission lines in the rest-frame ultraviolet and optical. Here, we apply a machine learning technique known as lasso regression on mock JWST/NIRSpec observations of simulated z = 7 galaxies in order to obtain a model that can predict the escape fraction from JWST/NIRSpec data. Barring systematic biases in the simulated spectra, our method is able to retrieve the escape fraction with a mean absolute error of

for spectra with signal-to-noise ratio ≈ 5 at a rest-frame wavelength of 1500 Å for our fiducial simulation. This prediction accuracy represents a significant improvement over previous similar approaches.
Congenital Nasolacrimal Duct Obstruction: Comparison of Two Different Treatment Algorithms
Authors: Napier Maria L. , Armstrong David J. , McLoone Sean F. , McLoone Eibhlin M.
Published: August 2016

Purpose:

To clarify the most appropriate treatment regimen for congenital nasolacrimal duct obstruction (CNLDO).

Methods:

A retrospective observational analysis was performed of patients undergoing probing with or without intubation to treat CNLDO in a single institution (Royal Victoria Hospital, Belfast) from 2006 to 2011.

Results:

Based on exclusion criteria, 246 eyes of 177 patients (aged 0 to 9.8 years with a mean age of 2.1 years) were included in this study: 187 (76%) eyes had successful outcome at first intervention with primary probing, whereas 56 (23%) eyes underwent secondary intervention. There were no significant differences by gender, age, or obstruction complexity between the successful and unsuccessful patients with first intervention. For those patients requiring secondary intervention, 16 of 24 (67%) eyes had successful probing, whereas 22 of 24 (92%) had successful intubation. Patients with intubation as a secondary procedure were significantly more likely to have a successful outcome (
P
= .037). Statistical analysis was performed using the Fisher's exact test and Barnard's exact test.

Conclusions:

Primary probing for CNLDO has a high success rate that is not adversely affected by increasing age. This study also indicates that if initial probing is unsuccessful, nasolacrimal intubation rather than repeat probing yields a significantly higher success rate.

[
J Pediatr Ophthalmol Strabismus
. 2016;53(5):285–291.]
Familial Exudative Vitreoretinopathy With a Novel
LRP5
Mutation
Authors: Pefkianaki Maria , Hasanreisoglu Murat , Suchy Sharon F. , Shields Carol L.
Published: August 2016

This report reviews the genetics of familial exudative vitreoretinopathy (FEVR) and describes the identification of a novel variant in the
LRP5
gene. A 20-month-old boy presented with reduced visual acuity in the right eye from exudative retinal detachment with mild retinal traction. Fluorescein angiography in the right eye disclosed extensive peripheral retinal non-perfusion and telangiectatic vessels and the left eye showed minimal peripheral non-perfusion. These features were suggestive of FEVR. Treatment with laser photocoagulation and cryotherapy to the region of non-perfusion was performed with resolution of the exudative retinal detachment. Fundus examination of the father revealed mild signs of FEVR, such as hyperacute retinal vascular branching and slight retinal vascular traction, whereas the mother's fundus examination was unremarkable. Genetic testing revealed that the affected boy was negative for mutations in the
FZD4
,
NDP
, and
TSPAN12
genes and heterozygous for a previously unreported A745V variant in the
LRP5
gene. The father was also heterozygous for the A745V variant in the
LRP5
gene and the unaffected mother showed no mutation. A genetic evaluation of the known genes associated with FEVR revealed a novel variant in the
LRP5
gene that co-segregated with the phenotype in the family.

[
J Pediatr Ophthalmol Strabismus
. 2016;53:e39–e42.]