bluuebunny/crossref_metadata_2025 huggingface dataset:
The CD63 homologs, Tsp42Ee and Tsp42Eg, restrict endocytosis and promote neurotransmission through differential regulation of synaptic vesicle pools
Authors: Hendricks Emily L. , Smith Ireland R. , Prates Bruna , Barmaleki Fatemeh , Liebl Faith L. W.
Published: August 2022

The Tetraspanin (Tsp), CD63, is a transmembrane component of late endosomes and facilitates vesicular trafficking through endosomal pathways. Despite being widely expressed in the human brain and localized to late endosomes, CD63's role in regulating endo- and exocytic cycling at the synapse has not been investigated. Synaptic vesicle pools are highly dynamic and disruptions in the mobilization and replenishment of these vesicle pools have adverse neuronal effects. We find that the CD63 homologs, Tsp42Ee and Tsp42Eg, are expressed at the Drosophila neuromuscular junction to regulate synaptic vesicle pools through both shared and unique mechanisms. Tsp42Ee and Tsp42Eg negatively regulate endocytosis and positively regulate neurotransmitter release. Both tsp mutants show impaired locomotion, reduced miniature endplate junctional current frequencies, and increased endocytosis. Expression of human CD63 in Drosophila neurons leads to impaired endocytosis suggesting the role of Tsps in endocytosis is conserved. We further show that Tsps influence the synaptic cytoskeleton and membrane composition by regulating Futsch loop formation and synaptic levels of SCAR and PI(4,5)P2. Finally, Tsp42Ee and Tsp42Eg influence the synaptic localization of several vesicle-associated proteins including Synapsin, Synaptotagmin, and Cysteine String Protein. Together, our results present a novel function for Tsps in the regulation of vesicle pools and provide insight into the molecular mechanisms of Tsp-related synaptic dysfunction.
Modeling analysis of subthreshold voltage signaling along hippocampal mossy fiber axons
Authors: Kamiya Haruyuki
Published: August 2022

Axons are classically thought of as electrically well isolated from other parts of the neurons due to the shape of a long cable-like structure. In contrast to this classical view on axonal compartmentalization, recent studies revealed that subthreshold depolarization of soma and dendrite passively propagates to the axons for a substantial distance, as demonstrated in some experimentally accessible axons including hippocampal mossy fibers and cortical pyramidal cell axons. Passive propagation of subthreshold dendritic EPSPs to the axons, defined as EPreSPs (excitatory presynaptic potentials), has been demonstrated to affect transmitter release from the axon terminals. To further characterize and explore the functional significance of passive subthreshold voltage signaling along the axons, the model of EPreSPs along hippocampal mossy fibers, proposed by Alle and Geiger, was reconstructed on the NEURON simulator. To test the effect of EPreSPs on action potentials and transmitter release from the axon terminals, additional conductances were incorporated into the previous passive propagation model. These include the axonal sodium, potassium, and leak channels as well as presynaptic calcium channels composed of P/Q-, N-, and R-types, which are reconstructed from the properties of those recorded from mossy fiber boutons experimentally. In this revised model, the preceding subthreshold EPreSPs slightly reduced the action potential-evoked presynaptic calcium currents by a decrease in the amplitude of action potentials due to the slow depolarization. It should be mentioned that EPreSPs by themselves elicited small calcium currents during subthreshold depolarization through these high-voltage activated calcium channels. Since the previous experimental study by simultaneous pre and postsynaptic recordings demonstrated that EPreSPs enhanced action potential-evoked transmitter release from the mossy fiber terminals, it has been suggested that different mechanisms from the enhancement of action potential-evoked presynaptic calcium entry may involve enhanced transmitter release by EPreSP. Small calcium entry by subthreshold EPreSPs may enhance transmitter release from the mossy fiber terminals by acting as high-affinity calcium sensors for enhancing transmitter release. Another form of axonal subthreshold voltage signaling, GABA-EPreSPs elicited by a spillover of GABA from surrounding interneurons, was also explored. Functional consequences of the two modes of axonal subthreshold voltage signaling were discussed with the simulation results.
Diastolic blood pressure predicts enlarged vertebral venous plexus and intracranial pressure in patients with bilateral transverse sinus stenosis
Authors: Li Min , Gao Xiaogang , Liu Fengwei , Sun Jingkun , Xia Ning , Meng Ran , Ji Xunming
Published: August 2022

Background

Bilateral transverse sinus stenosis (BTSS) is associated with intracranial hypertension. Enlarged vertebral venous plexus (EVVP) refers to a compensation mechanism against elevated intracranial pressure (ICP) in patients with BTSS. This study aims to investigate the influencing factors of EVVP.

Methods

Patients with BTSS were prospectively recruited from the neurology department and neurosurgery department of Xuanwu Hospital Capital Medical University from January 2020 to December 2021.

Results

A total of 37 patients were enrolled with a mean age of 45.42 ± 15.64 years. Women tend to be more susceptible to BTSS. The most common co-morbid disease was hypertension. The most common clinical manifestations were visual disorders, headaches, and tinnitus. BMI and DBP were significantly higher in BTSS patients without EVVP than those with EVVP. Multivariate analysis revealed that diastolic blood pressure (DBP) was negatively correlated with EVVP. In addition, a positive correlation between DBP and the ICP was also observed. A DBP of 81.5 mmHg was calculated as the cutoff value for the presence of EVVP. BTSS patients with DBP ≤ 81.5 mmHg had a higher incidence of EVVP and a lower ICP compared to those with DBP > 81.5 mmHg.

Conclusions

DBP was identified as an independent predictor of EVVP. DBP was lower (≤81.5 mmHg) in patients with EVVP and therefore was associated with a lower ICP in patients with BTSS.
Spatiotemporal analysis of regional TIA trends
Authors: Kawai Andrew , Hui Samuel , Beare Richard , Srikanth Velandai K. , Sundararajan Vijaya , Ma Henry , Phan Thanh G.
Published: August 2022

Background

There has been a decline in the stroke incidence across high income countries but such knowledge exists at Country or State rather than areal unit level such local government area (LGA). In this disease mapping study, we evaluate if there are local hot spots or temporal trends in TIA rate. Such knowledge will be of help in planning healthcare service delivery across regions.

Methods

Linked hospital discharge data (Victorian Admitted Episodes Dataset or VAED) was used to collect TIA (defined by ICD-10-AM codes G450-G459) cases from 2001 to 2011. The State of Victoria is the second most populous state in Australia, with a population of 6.7 million and can be divided into 79 administrative units or LGA. The data is anonymized and contains residence of the patient in terms of LGA but not exact location. The date of the TIA event when the patient is admitted to hospital is provided in the dataset. The number of TIAs per year was aggregated for each LGA. Standardized TIA ratios were calculated by dividing actual over expected cases for each LGA per year. We used Integrated Nested Laplace Approximation (INLA) to perform spatial and spatiotemporal regression, adjusting for hypertension, sex and population, age (≥60), and socio-economic status (SES) decile within the LGA. The final model was chosen based on the lowest the Deviance Information Criterion (DIC) and Watanabe-Akaike information criteria (WAIC).

Results

Choropleth maps showed a higher standardized TIA ratios in North-West rural region. Compared to the baseline model (DIC 13,159, WAIC 13,261), adding in a spatial random effect significantly improved the model (DIC 6,463, WAIC 6,667). However, adding a temporal component did not lead to a significant improvement (DIC 6,483, WAIC 6,707).

Conclusion

Our finding suggests a statically significant spatial component to TIA rate over regional areas but no temporal changes or yearly trends. We propose that such exploratory method should be followed by evaluation of reasons for regional variations and which in turn can identify opportunities in primary prevention of stroke, and stroke care.
Profiles of total and sn-2 fatty acid of human mature milk and their correlated factors: A cross-sectional study in China
Authors: Ni Mengmei , Wang Yingyao , Yang Zhirui , Xu Xuebing , Zhang Hong , Yang Yuexin , Zhang Lishi , Chen Jinyao
Published: August 2022

Fatty acid (FA) in breast milk is beneficial to the growth and neurodevelopment of infants. However, the structure profiles of breast milk FAs and the influencing factors which are crucial for normal function have not been fully elucidated. This study aimed to characterize the profiles of total and sn-2 FAs in human mature milk based on two representative urban areas in China and explore potential sociodemographic determinants. Mothers (n = 70) at 40–100 d postpartum from Beijing and Danyang were recruited according to unified inclusion and exclusion criteria. Total and sn-2 FA compositions were examined by gas chromatography and quantified. Using the Spearman correlation and multiple regression model, we found that the location and maternal education level were the most conspicuous correlated factor. The milk of mothers from Beijing had higher levels of the n-6 series of long-chain polyunsaturated fatty acids (LCPUFA) (C20:2, C20:3n-6, C20:4n-6, n-6PUFA/n-3PUFA, LA/ALA, and ARA/DHA) than that of Danyang, while the opposite was observed in the n-3 series of LCPUFA (C18:3n-3 and Total n-3PUFA). Compared to the milk of mothers with a high school degree or below, those with a bachelor's degree or above had lower SFAs (C10:0, C12:0, C14:0, and Total SFA), n-3 series of LCPUFA (C18:3n-3 and Total n-3PUFA), C18:1n-9t, and higher n-6 series of LCPUFA (C18:2n-6c, C20:2, C20:4n-6, Total n-6PUFA, and n-6PUFA/n-3PUFA). Maternal age, infant gender, pre-conception body mass index (BMI), parity, delivery mode, and gestational weight gain were also associated with total FAs. However, fewer associations were found between the above factors and sn-2 FAs. This study will promote an understanding of human breast milk's lipid profile and help develop a formula more suitable for infants.
Nitrogen balance and outcomes in critically ill patients: A systematic review and meta-analysis
Authors: Zhu Yi-Bing , Yao Yan , Xu Yuan , Huang Hui-Bin
Published: August 2022

Objective

Nitrogen balance (NB) is a commonly used nutrition indicator in clinical practice, while its relation to the interpretation of protein malnutrition and outcomes in critically ill patients remains unclear. This study aimed to evaluate the impact of NB on prognosis in such a patient population.

Methods

We searched for relevant studies in PubMed, EMBASE, and the Cochrane Database up to May 10, 2022. Meta-analyses were performed to evaluate the relationship between NB (initial, final, or absolute change of NB levels) and prognosis and important clinical outcomes in critically ill patients. Pooled odds ratios (ORs) and mean differences (MDs) together with their 95% confidence intervals (CIs) were calculated. We also conducted subgroup analyses to explore the sources of heterogeneity.

Results

Eight studies with 1,409 patients were eligible. These studies were moderate to high quality. When pooled, the initial NB was comparable between the survival and non-survival groups (five studies, MD 1.20, 95% CI, −0.70 to 3.11, I2 = 77%; P = 0.22), while a significantly higher final NB in the survival group than that in the death group (two studies, MD 3.69, 95% CI, 1.92–5.46, I2 = 55%; P < 0.0001). Two studies provided the absolute change of NB over time and suggested survival patients had more increased NB (MD 4.16 g/day, 95% CI, 3.70–4.61, I2 = 0%; P < 0.00001). Similarly, for studies utilizing multivariate logistic regression, we found an improved NB (four studies, OR 0.85, 95% CI, 0.73–0.99, I2 = 61%; P = 0.04) but not an initial NB (two studies, OR 0.92, 95% CI 0.78–1.08, I2 = 55%; P = 0.31) was significantly associated the risk of all-cause mortality. These results were further confirmed in subgroup analyses. In addition, patients with improved NB had more protein and calorie intake and a similar length of stay in hospital than those without.

Conclusions

Our results suggested that an improved NB but not the initial NB level was associated with all-cause mortality in critically ill patients. This highlights the requirement for dynamic monitoring of NB during nutrition treatment. Further randomized clinical trials examining the impact of NB-guided protein intake on clinical outcomes in critically ill patients are warranted.

Systematic review registration

INPLASY202250134, https://doi.org/10.37766/inplasy2022.5.0134.
Multimodality imaging in lobular breast cancer: Differences in mammography, ultrasound, and MRI in the assessment of local tumor extent and correlation with molecular characteristics
Authors: Dołęga-Kozierowski Bartosz , Lis Michał , Marszalska-Jacak Hanna , Koziej Mateusz , Celer Marcin , Bandyk Małgorzata , Kasprzak Piotr , Szynglarewicz Bartłomiej , Matkowski Rafał
Published: August 2022

Introduction

Invasive lobular breast cancer (ILC) is a diagnostic challenge due to the diversity of morphological features. The objective of the study was to investigate the presentation and local extent of ILC using various imaging techniques and to assess the correlation between imaging and molecular profile.

Materials and methods

We reviewed 162 consecutive patients with ILC found on vacuum-assisted biopsy, who underwent evaluation of the lesion morphology and extent using ultrasound (US), mammography (MMG), and magnetic resonance imaging (MRI). Radiographic features were compared with ILC intrinsic subtype based on the expression of Ki-67 and estrogen, progesterone, and HER2 receptors.

Results

A total of 113 mass lesions and 49 non-mass enhancements (NMEs) were found in MRI. Masses were typically irregular and spiculated, showing heterogeneous contrast enhancement, diffusion restriction, and type III enhancement curve. NMEs presented mainly as the area of focal or multiregional distribution with heterogeneous or clumped contrast enhancement, diffusion restriction, and type III enhancement curve. Lesion extent significantly varied between MRI and MMG/ultrasonography (USG) (P < 0.001) but did not differ between MGF and ultrasonography (USG). The larger the ILC, the higher the disproportion when lesion extent in MRI was compared with MMG (P < 0.001) and ultrasonography (USG) (P < 0.001). In the study group, there were 97 cases of luminal A subtype (59.9%), 54 cases of luminal B HER2− (33.3%), nine cases of luminal B HER2+ (5.5%), and two cases of triple negative (1.2%). The HER2 type was not found in the study group. We did not observe any significant correlation between molecular profile and imaging.

Conclusion

MRI is the most effective technique for the assessment of ILC local extent, which is important for optimal treatment planning. Further studies are needed to investigate if the intrinsic subtype of ILC can be predicted by imaging features on MRI.
PKIB involved in the metastasis and survival of osteosarcoma
Authors: Wan Rongxue , Yang Gu , Liu Qianzhen , Fu Xiaokang , Liu Zengping , Miao Huilai , Liu Huan , Huang Wenhua
Published: August 2022

Osteosarcoma is frequently metastasized at the time of diagnosis in patients. However, the underlying mechanism of osteosarcoma metastasis remains poorly understood. In this study, we evaluated DNA methylation profiles combined with gene expression profiles of 21 patients with metastatic osteosarcoma and 64 patients with non-metastatic osteosarcoma from TARGET database and identified PKIB and AIM2 as hub genes related to the metastasis of osteosarcoma. To verify the effects of PKIB on migration and invasion of osteosarcoma, we performed wound-healing assay and transwell assay. The results showed that PKIB significantly inhibited the migration and invasion of osteosarcoma cells, and the Western blot experiments showed that the protein level of E-cad was upregulated and of VIM was downregulated in 143-B cell recombinant expression PKIB. These results indicate that PKIB inhibit the metastasis of osteosarcoma. CCK-8 assay results showed that PKIB promote the proliferation of osteosarcoma. In addition, the Western blot results showed that the phosphorylation level of Akt was upregulated in 143-B cells overexpressing PKIB, indicating that PKIB promotes the proliferation of osteosarcoma probably through signaling pathway that Akt involved in. These results give us clues that PKIB was a potential target for osteosarcoma therapy. Furthermore, combined clinical profiles analysis showed that the expression of AIM2- and PKIB- related risk scores was significantly related to the overall survival of patients with osteosarcoma. Thus, we constructed a nomogram based on AIM2 and PKIB expression–related risk scores for osteosarcoma prognostic assessment to predict the 1-, 2-, 3-, and 5-year overall survival rate of patients with metastatic osteosarcoma, assisting clinicians in the diagnosis and treatment of metastatic osteosarcoma.
Deep learning models for predicting the survival of patients with chondrosarcoma based on a surveillance, epidemiology, and end results analysis
Authors: Yan Lizhao , Gao Nan , Ai Fangxing , Zhao Yingsong , Kang Yu , Chen Jianghai , Weng Yuxiong
Published: August 2022

Background

Accurate prediction of prognosis is critical for therapeutic decisions in chondrosarcoma patients. Several prognostic models have been created utilizing multivariate Cox regression or binary classification-based machine learning approaches to predict the 3- and 5-year survival of patients with chondrosarcoma, but few studies have investigated the results of combining deep learning with time-to-event prediction. Compared with simplifying the prediction as a binary classification problem, modeling the probability of an event as a function of time by combining it with deep learning can provide better accuracy and flexibility.

Materials and methods

Patients with the diagnosis of chondrosarcoma between 2000 and 2018 were extracted from the Surveillance, Epidemiology, and End Results (SEER) registry. Three algorithms—two based on neural networks (DeepSurv, neural multi-task logistic regression [NMTLR]) and one on ensemble learning (random survival forest [RSF])—were selected for training. Meanwhile, a multivariate Cox proportional hazards (CoxPH) model was also constructed for comparison. The dataset was randomly divided into training and testing datasets at a ratio of 7:3. Hyperparameter tuning was conducted through a 1000-repeated random search with 5-fold cross-validation on the training dataset. The model performance was assessed using the concordance index (C-index), Brier score, and Integrated Brier Score (IBS). The accuracy of predicting 1-, 3-, 5- and 10-year survival was evaluated using receiver operating characteristic curves (ROC), calibration curves, and the area under the ROC curves (AUC).

Results

A total of 3145 patients were finally enrolled in our study. The mean age at diagnosis was 52 ± 18 years, 1662 of the 3145 patients were male (53%), and mean survival time was 83 ± 67 months. Two deep learning models outperformed the RSF and classical CoxPH models, with the C-index on test datasets achieving values of 0.832 (DeepSurv) and 0.821 (NMTLR). The DeepSurv model produced better accuracy and calibrated survival estimates in predicting 1-, 3- 5- and 10-year survival (AUC:0.895-0.937). We deployed the DeepSurv model as a web application for use in clinical practice; it can be accessed through https://share.streamlit.io/whuh-ml/chondrosarcoma/Predict/app.py.

Conclusions

Time-to-event prediction models based on deep learning algorithms are successful in predicting chondrosarcoma prognosis, with DeepSurv producing the best discriminative performance and calibration.
Design and test a converging and de Laval nozzle using additive manufacturing
Authors: Chen Mingtai , Baby Ruksana , Dillard Seth , Lee Yi Tsung , Ekkad Srinath
Published: August 2022

The advent of additive manufacturing technology has facilitated the design and fabrication of parts and models in both academia and aerospace industry. Compressible flow in the nozzles is not a new research topic; however, the accuracy of the experimental results obtained from the nozzles using additive manufacturing has not been assessed comprehensively. Surface roughness and strength of 3D-printed nozzles are two major concerns when they are applied to compressible flows. In this paper, a converging and a de Laval nozzle fabricated by additive manufacturing using ABS filament are designed and tested. Surface roughness inside the converging nozzle is quantified using a nondestructive method. In general, the experimental results compare well with the analytical solutions from isentropic equations for the converging nozzle and the numerical simulations conducted in ANASYS Fluent for the de Laval nozzle. 3D-printed nozzles can be employed to quickly demonstrate and verify novel ideas and concepts in the pedagogy and research at large Reynolds numbers.
Validation of the Gait Disorientation Test in children with concussion
Authors: Alkathiry Abdulaziz A. , Alsubaie Saud F. , Alsalaheen Bara A. , Whitney Susan L.
Published: August 2022

Background

Mild traumatic brain injury (mTBI) or concussion is a complex injury that is difficult to diagnose and assess. There are negative impacts on cognition, balance, and mobility after a concussion. The Gait Disorientation Test (GDT) is an objective measure that assesses a person's balance ability by comparing the walking time with eyes open and the walking time with eyes closed in a standardized walking task. The purpose of this study was to assess the validity and the diagnostic properties of the GDT in children with concussions.

Methods

Thirty-six children with concussions, and 91 controls aged between 9 and 18 years old participated in the study. Participants completed demographics, the GDT, the Functional Gait Assessment (FGA), the Pediatric Vestibular Symptom Questionnaire (PVSQ), and the Pediatric Visually Induced Dizziness Questionnaire (PVID).

Results

Children with concussions showed higher (worse) GDT scores (M = 2.18 ± 1.93 s) than healthy controls (M = 1.13 ± 0.95 s), which was statistically significant (P = 0.014).

Conclusion

The GDT was able to distinguish between children with concussions and healthy controls. Given the simplicity of the GDT, it can be used to assist in discriminating between children with and without concussion.
Recommendations for nasotracheal tube insertion depths in neonates
Authors: Ebenebe Chinedu Ulrich , Schriever Kristina , Wolf Monika , Herrmann Jochen , Singer Dominique , Deindl Philipp
Published: August 2022

Background

Endotracheal tube (ETT) malposition is common in neonatal intubation. Recommendations for ETT insertion depths predominantly address orotracheal intubation. The aim of this study was to develop gestational age-, weight-, and length-based curve charts and tables for nasotracheal ETT insertion depth recommendations in neonates.

Method

In this retrospective single-center study, the individual optimal ETT insertion depths in neonates were determined by evaluating postintubation radiographic images. Gestational age-, weight-, and length-based best-fit curves and tables were generated using regression analysis to calculate related ETT insertion depths. The insertion depths predicted by the models were compared with previously published recommendations.

Results

We analyzed intubations of 178 neonates (gestational age range at intubation: 23.7–43.0 weeks). Applying sigmoidal logistic regression models, curves, and tables revealed R2 values between 0.766 and 0.837. The insertion depths predicted by the models revealed certain deviations when compared with four previously published recommendations for nasotracheal ETT depth estimation in neonates.

Conclusion

The charts and tables developed in this study enable a fast and accurate determination of recommended nasotracheal ETT insertion depths in neonates.
Atezolizumab plus platinum-based chemotherapy as first-line therapy for metastatic urothelial cancer: A cost-effectiveness analysis
Authors: Liu Xiaoyan , Lang Yitian , Chai Qingqing , Lin Yan , Liao Yahui , Zhu Yizhun
Published: August 2022

Purpose: According to the IMvigor130 trial, adding atezolizumab to platinum-based chemotherapy was effective in the treatment of metastatic urothelial cancer (mUC). Based on the perspective of the United States and China, the current study evaluated cost-effectiveness of atezolizumab plus chemotherapy for mUC patients in the first-line setting.

Methods: A partitioned survival model was adopted for mUC patients. The survival data were derived from the IMvigor130 trial. Direct cost values were collected from the Centers for Medicare and Medicaid Services (CMS), Chinese Drug Bidding Database, and published literatures. The utility and toxicity data were gathered from related research studies and IMvigor130 trial. The incremental cost–utility ratios (ICURs) and incremental cost-effectiveness ratios (ICERs) were calculated and analyzed. Scenario analyses and sensitivity analyses were performed to observe the outputs and uncertainties.

Results: The base-case analysis showed that the ICUR of atezolizumab plus chemotherapy versus chemotherapy in American and Chinese settings is $ 737,371 /QALY and $ 385,384 /QALY, respectively. One-way sensitivity analyses showed that the ICUR ranged from $ 555,372/QALY to $ 828,205/QALY for the United States. Also, the range was from $ 303,099/QALY to $ 433,849/QALY in the Chinese setting. A probabilistic sensitivity analysis showed the likelihood that atezolizumab plus chemotherapy becoming the preferred strategy was a little low even if the price reduction strategy was applied.

Conclusion: Adding atezolizumab to chemotherapy improved survival time, but it is not a cost-saving option compared to chemotherapy for metastatic urothelial cancer patients in the American and Chinese settings.
Efficacy and safety of intermittent theta-burst stimulation in patients with schizophrenia: A meta-analysis of randomized sham-controlled trials
Authors: Goh Kah Kheng , Chen Chun-Hsin , Wu Tzu-Hua , Chiu Yi-Hang , Lu Mong-Liang
Published: August 2022

Theta-burst stimulation is a non-invasive brain stimulation technique that was introduced as a potential augmentation treatment for patients with schizophrenia. The purpose of this meta-analysis was to investigate the therapeutic efficacy and safety of intermittent theta-burst stimulation in patients with schizophrenia. Following the PRISMA guidelines, the MEDLINE, Embase, Cochrane, Scopus, Web of Science, and CNKI databases were searched for relevant studies from database inception to 9 January 2022. Change in symptom severity among patients with schizophrenia was the primary outcome, and changes in cognitive function and safety profiles, including the discontinuation rate and adverse events, were secondary outcomes. In total, 13 double-blind randomized sham-controlled trials with 524 patients were included. Intermittent theta-burst stimulation adjunct to antipsychotics was associated with significantly improved psychopathology in patients with schizophrenia, particularly for negative symptoms and general psychopathology but not for positive symptoms or cognitive function. The stimulation parameters influenced the effectiveness of intermittent theta-burst stimulation. A more favorable effect was observed in patients who received theta-burst stimulation at the left dorsolateral prefrontal cortex, with ≥1800 pulses per day, for ≥20 sessions, and using an inactive sham coil as a placebo comparison in the study. The intermittent theta-burst stimulation is well tolerated and safe in patients with schizophrenia. Intermittent theta-burst stimulation adjunct to antipsychotics treatment is associated with significant improvement in negative symptoms and favorable tolerability in patients with schizophrenia. This meta-analysis may provide insights into the use of intermittent theta-burst stimulation as an additional treatment to alleviate the negative symptoms of schizophrenia.
Anger can make fake news viral online
Authors: Chuai Yuwei , Zhao Jichang
Published: August 2022

Fake news that manipulates political elections, strikes financial systems, and even incites riots is more viral than real news online, resulting in unstable societies and buffeted democracy. While factor that drives the viral spread of fake news is rarely explored. In this study, it is unexpectedly found that the easier contagion of fake news online is positively associated with the greater anger it carries. The same results in Twitter and Weibo indicate that this correlation is independent of the platform. Moreover, mutations in emotions like increasing anger will progressively speed up the information spread. Increasing the occupation of anger by 0.1 and reducing that of joy by 0.1 are associated with the generation of nearly six more retweets in the Weibo dataset. Offline questionnaires reveal that anger leads to more incentivized audiences in terms of anxiety management and information sharing and accordingly makes fake news more contagious than real news online. Cures such as tagging anger in social media could be implemented to slow or prevent the contagion of fake news at the source.
New permanent bundle-branch block and long-term prognosis of patients with new onset ST-elevation myocardial infarction who underwent percutaneous coronary intervention
Authors: Yang Yi , Wang Jun , Wu Bing , Xu Yanan , Tang Long , Jiang Haibing , Wang Benfang , Zhu Tongjian
Published: August 2022

Aim: The aim of the study was to evaluate the potential predictive value of permanent RBBB and LBBB for longer-term prognosis in patients with new-onset STEMI who underwent percutaneous coronary intervention (PCI).

Methods: Patients with new-onset STEMI that underwent emergency PCI at our department from June 2012 to September 2020 were included in the study. Gensini score (GS) was employed to evaluate the severity of coronary lesions. The primary endpoint of the study was the occurrence of major adverse cardiac and cerebrovascular events (MACCEs), the composite of cardiac mortality, recurrence of myocardial infarction, cardiac shock, stroke, stent thrombosis, or revascularization. We also set all-cause mortality as a secondary endpoint.

Results: Out of the 547 patients, 29 patients had new-onset permanent LBBB, 51 patients had new-onset permanent RBBB, and 467 patients had no bundle-branch block (BBB). The occurrence of no BBB, new permanent LBBB, or RBBB was not associated with the severity of coronary artery lesions as evaluated by the GS. After follow-up at an average of 43.93 months, MACCEs occurred in 52 patients. Kaplan-Meier analysis showed that patients with new-onset RBBB were at greater risk for MACCEs compared to those with new onset LBBB (χ2 = 5.107, p = 0.021). Also, an independent correlation was found between new permanent RBBB and LBBB and MACCEs risk. The adjusted hazard ratios (HRs) were 6.862 [95% confidence interval (CI) of 3.764–12.510] for the new-onset permanent RBBB and 3.395 (95% CI of 1.280–9.005) for LBBB, compared to those with no BBB, respectively (both p < 0.05).

Conclusion: New onset permanent RBBB in patients with new onset STEMI who underwent PCI may be correlated independently with increased risk of poor long-term prognosis.
Impact of polyploidy on plant tolerance to abiotic and biotic stresses
Authors: Tossi Vanesa E. , Martínez Tosar Leandro J. , Laino Leandro E. , Iannicelli Jesica , Regalado José Javier , Escandón Alejandro Salvio , Baroli Irene , Causin Humberto Fabio , Pitta-Álvarez Sandra Irene
Published: August 2022

Polyploidy, defined as the coexistence of three or more complete sets of chromosomes in an organism’s cells, is considered as a pivotal moving force in the evolutionary history of vascular plants and has played a major role in the domestication of several crops. In the last decades, improved cultivars of economically important species have been developed artificially by inducing autopolyploidy with chemical agents. Studies on diverse species have shown that the anatomical and physiological changes generated by either natural or artificial polyploidization can increase tolerance to abiotic and biotic stresses as well as disease resistance, which may positively impact on plant growth and net production. The aim of this work is to review the current literature regarding the link between plant ploidy level and tolerance to abiotic and biotic stressors, with an emphasis on the physiological and molecular mechanisms responsible for these effects, as well as their impact on the growth and development of both natural and artificially generated polyploids, during exposure to adverse environmental conditions. We focused on the analysis of those types of stressors in which more progress has been made in the knowledge of the putative morpho-physiological and/or molecular mechanisms involved, revealing both the factors in common, as well as those that need to be addressed in future research.
Many paths to one goal: Identifying integrated rice root phenotypes for diverse drought environments
Authors: Fonta Jenna E. , Vejchasarn Phanchita , Henry Amelia , Lynch Jonathan P. , Brown Kathleen M.
Published: August 2022

Drought is a major source of yield loss in the production of rice (Oryza sativaL.), and cultivars that maintain yield under drought across environments and drought stress scenarios are urgently needed. Root phenotypes directly affect water interception and uptake, so plants with root systems optimized for water uptake under drought would likely exhibit reduced yield loss. Deeper nodal roots that have a low metabolic cost per length (i.e., cheaper roots)viasmaller root diameter and/or more aerenchyma and that transport water efficiently through smaller diameter metaxylem vessels may be beneficial during drought. Subsets of the Rice Diversity Panel 1 and Azucena × IR64 recombinant inbred lines were grown in two greenhouse and two rainout shelter experiments under drought stress to assess their shoot, root anatomical, and root architectural phenotypes. Root traits and root trait plasticity in response to drought varied with genotype and environment. The best-performing groups in the rainout shelter experiments had less plasticity of living tissue area in nodal roots than the worst performing groups. Root traits under drought were partitioned into similar groups or clustersviathe partitioning-around-medoids algorithm, and this revealed two favorable integrated root phenotypes common within and across environments. One favorable integrated phenotype exhibited many, deep nodal roots with larger root cross-sectional area and more aerenchyma, while the other favorable phenotype exhibited many, deep nodal roots with small root cross-sectional area and small metaxylem vessels. Deeper roots with high theoretical axial hydraulic conductance combined with reduced root metabolic cost contributed to greater shoot biomass under drought. These results reflect how some root anatomical and architectural phenes work in concert as integrated phenotypes to influence the performance of plant under drought stress. Multiple integrated root phenotypes are therefore recommended to be selected in breeding programs for improving rice yield across diverse environments and drought scenarios.
Chromosomal instability and phenotypic variation in a specific lineage derived from a synthetic allotetraploid wheat
Authors: Lv Ruili , Wang Changyi , Wang Ruisi , Wang Xiaofei , Zhao Jing , Wang Bin , Aslam Tariq , Han Fangpu , Liu Bao
Published: August 2022

Newly formed plant allopolyploids usually have meiosis defect, resulting in chromosomal instability manifested as variation in chromosome number and/or structure. However, not all nascent allopolyploids are equally unstable. The wheat group (Aegilops/Triticum) contains 13 diploid species with distinct genome types. Many of these species can be artificially hybridized to produce viable but sterile inter-specific/intergeneric F1 hybrids, which can generate fertile synthetic allotetraploid wheats after whole genome doubling. Compared with synthetic allotetraploid wheats that contain genome combinations of AADD and S*S*DD (S* refers to related S genomes of a different species), those containing an S*S*AA genome are significantly more stable. However, robustness of the relative stability of S*S*AA genomes is unknown, nor are the phenotypic and fitness consequences during occurrences of secondary chromosomal instability. Here, we report a specific lineage originated from a single individual plant of a relatively stable synthetic allotetraploid wheat with genomes SlSlAA (Sl and A subgenomes were from Ae. longissima and T. urartu, respectively) that showed a high degree of transgenerational chromosomal instability. Both numerical chromosome variation (NCV) and structural chromosome variation (SCV) occurred widely. While substantial differences in frequencies of both NCV and SCV were detected across the different chromosomes, only NCV frequencies were significantly different between the two subgenomes. We found that NCVs and SCVs occurred primarily due to perturbed meiosis, allowing formation of multivalents and univalents as well as homoeologous exchanges. Thus, the combination of NCVs and SCVs affected multiple phenotypic traits, particularly those related to reproductive fitness.
A high-efficiency trichome collection system by laser capture microdissection
Authors: Qin Wei , Li Yongpeng , Peng Bowen , Liu Hang , Chen Tiantian , Yan Xin , Zhang Yaojie , Wang Chen , Yao Xinghao , Fu Xueqing , Li Ling , Tang Kexuan
Published: August 2022

Trichomes, which are classified as glandular or non-glandular, are hair-like epidermal structures that are present on aerial parts of most plant species. Glandular secretory trichomes (GSTs) have the capacity to secrete and store specialized metabolites, which are widely used as natural pesticides, food additives, fragrance ingredients or pharmaceuticals. Isolating individual trichomes is an essential way for identifying trichome-specific gene functions and discovering novel metabolites. However, the isolation of trichomes is difficult and time-consuming. Here, we report a method to isolate the GSTs from leaf epidermis dispense with fixation using laser capture microdissection (LCM). In this study, 150 GSTs were captured efficiently from Artemisia annua leaves and enriched for artemisinin measurement. UPLC analysis of microdissected samples indicated specific accumulation of secondary metabolites could be detected from a small number of GSTs. In addition, qRT-PCR revealed that the GST-specific structural genes involved in artemisinin biosynthesis pathway were highly expressed in GSTs. Taken together, we developed an efficient method to collect comparatively pure GSTs from unfixed leaved, so that the metabolites were relatively obtained intact. This method can be implemented in metabolomics research of purely specific plant cell populations and has the potential to discover novel secondary metabolites.
Combined effect of game position and body size on network-based centrality measures performed by young soccer players in small-sided games
Authors: Borges Paulo Henrique , da Costa Julio Cesar , Ramos-Silva Luiz Fernando , Praça Gibson Moreira , Ronque Enio Ricardo Vaz
Published: August 2022

This study verified the effects of body size and game position on interactions performed by young soccer players in small-sided games (SSG). The sample consisted of 81 Brazilian soccer players (14.4 ± 1.1 years of age). Height, body mass, and trunk-cephalic height were measured. SSG was applied in the GK + 3v3 + GK format, and Social Network Analyses were carried out through filming the games to obtain the following prominence indicators: degree centrality, closeness centrality, degree prestige, and proximity prestige, in addition to network intensity and number of goals scored. Factorial ANCOVA (bone age as covariate) was used to test the effects of game position, body size, and respective interaction on centrality measurements (p < 0.05). Similarity between game positions in body size indicators (p > 0.05) was observed. The game position affected degree centrality (p = 0.01, η2 = 0.16), closeness centrality (p  = 0.01, η2 = 0.11), and network intensity (p = 0.02, η2 = 0.09), in which midfielders presented the highest network prominence values when compared to defenders and forwards. In conclusion, midfielders are players with high interaction patterns in the main offensive plays, which behavior is independent of body size.
How to motivate planners to participate in community micro-renewal: An evolutionary game analysis
Authors: Wang Dong , Wu Meiling , Qu Jiulong , Fan Yuncui
Published: August 2022

In China, grassroots governments have attempted to introduce community planners into community micro-renewal, using their expertise to guide the design and implementation of community micro-renewal. However, issues remain to be studied and resolved, including how to effectively play the community planner role to coordinate multiple parties’ interests. This study constructed an evolutionary game model based on the behaviors of multiple subjects with participation by community planners, to establish the payment functions on both sides of the game under different choice strategies; explore evolutionary stabilization strategies by replication dynamic equations; and to analyze the conditions for the multi-party evolutionary game to reach the ideal stable state. The findings: (1) Show that financial subsidies provided by the grassroots government to community planners have a positive effect on the latter’s behavioral choices; (2) Illustrate the path of the tripartite evolutionary game among the grassroots government, residents, and community planners to reach ideal stability (incentive, active participation, and positive promotion); and (3) Describe how the project benefits from community planners promoting community micro-renewal can effectively promote their positive behavioral choices.
COVID-19, students satisfaction about e-learning and academic achievement: Mediating analysis of online influencing factors
Authors: Younas Muhammad , Noor Uzma , Zhou Xiaoyong , Menhas Rashid , Qingyu Xu
Published: August 2022

Background

The current study examines student satisfaction with e-learning, the adaption of online learning channels, digital competency of students' involvement, and academic achievement during COVID-19.

Purpose

The purpose of this study is to examine the online influencing components for learning among University students in Pakistan during the COVID-19 Pandemic.

Methods

The study population comprised Pakistani University students in Punjab province who tooke online lessons throughout the epidemic. In accordance with the study's purpose, a questionnaire survey was employed to gather primary data. SPSS-23 is used for analyzing the demographic data, and cleaning and preparing data for testing hypotheses. SmartPLS 3.0 was used to investigate the suggested study framework using structural equation modeling (SEM).

Results

The analysis of the SEM model shows that all planned hypotheses (Adaptation of Online Education Channels -> Satisfaction about E-learning, COVID-19 Pandemic -> Adaptation of Online Education Channels, COVID-19 Pandemic -> Digital Competence, COVID-19 Pandemic -> Motivation for Online Learning, COVID-19 Pandemic -> Willingness for Online Learning, Digital Competence -> Satisfaction about E-learning, Motivation for Online Learning -> Satisfaction about E-learning, Satisfaction about E-learning -> Academic Achievement, Willingness for Online Learning -> Satisfaction about E-learning) are confirmed.

Conclusion

The results linked e-learning satisfaction to academic success and Pakistani students who utilized e-learning throughout the outbreak reported higher levels of academic satisfaction and achievement.
Under the humble mask: Investigating when and how leader-expressed humility leads to employee voice
Authors: Zou Wen-Qian , Chen Shu-Chen
Published: August 2022

This study aimed to explore the psychological mechanisms through which psychological safety and self-efficacy mediate the relationship between leader-expressed humility and employee voice. Moreover, attribution theory was applied to examine the possible detrimental effects of leader-expressed humility when employees perceive manipulative intentions in their humble leader. The current study proposed the leader’s manipulative intention as a moderator to weaken the indirect relationships between leader-expressed humility and employee voice through psychological safety and self-efficacy. Time-lagged supervisor–subordinate matched data were used to test the model. Our findings reveal leader’s manipulative intention weakens the positive effect that leader-expressed humility impacts on employee voice through psychological safety and self-efficacy. The implications of the findings were discussed from both theoretical and practical perspectives.
Awe experiences, the sublime, and spiritual well-being in Arctic wilderness
Authors: Løvoll Helga Synnevåg , Sæther Knut-Willy
Published: August 2022

Experiences of awe can carry the potential for life-transforming experiences and foster awareness of nature as a lifelong value. How these experiences emerge was investigated empirically in a pristine natural environment and analyzed informed by a bottom-up perspective with an interdisciplinary understanding of environmental aesthetics and positive psychology. A group of Arctic nature guide students (n= 34) was followed on an 8-day advanced glacier course with additional learning topics related to the Arctic landscape and history, wildlife, and how to protect a wilderness camp from polar bear attacks. After this experience, students were invited to participate in the research project and were asked to reflect on their experiences immediately after their return to civilization. Two narratives each from 27 participants were collected, which provided 54 quotations for interpretation. Reflexive thematic analysis (RTA) surfaced three main themes: context, human response to encounters with nature, and transformation. The study of awe brings the tension between spirituality and well-being closer. The findings add empirical knowledge to the understanding of the contexts for these highly affective and complex feelings. The findings also add refined knowledge about the relationship between awe and the sublime. In transformation for human well-being, the role of self-knowledge or self-transcendence surfaced by wilderness experiences should not be underestimated.
An experimental investigation of association between children’s lying and behavior problems
Authors: Liu Xue , Shang Siyuan , Zanette Sarah , Zhang Yongkang , Sun Qingzhou , Sai Liyang
Published: August 2022

Children’s lying is a major concern for parents and teachers alike, not only because lying is an antisocial behavior but also because children’s lying correlates with other behavior problems, such as aggression and delinquency. Despite considerable correlational evidence demonstrating the relation between children’s lying and behavior problems, experimental evidence is scarce. This study uses a novel task to experimentally examine the relation between lying for personal reward and behavior problem symptoms among 9- to 11-year-old typically developed children (N = 275, 139 boys). Results revealed a positive correlation between children’s lying for personal reward and their behavior problem symptoms, and this correlation increases with age. Overall, this study provides experimental evidence suggesting children’s lying for personal reward is associated with behavior problems.