bluuebunny/crossref_metadata_2025 huggingface dataset:
Tungsten Thermionic Emission as a Gauge for Low Pressures of Cesium Vapor
Authors: Shida João , Wu Fangjian , Spieglan Eric , Çalışkan Mesut
Published: November 2020

Heated metal filaments under electric fields and low pressures of alkali metal gas eject electrons by thermionic emission as a function of the pressure of the gas and the temperature of the filament. To explore this process in a program to develop large-area alkali metal photocathodes, we have designed and built a gauge following the studies of Taylor and Langmuir. We present proof-of-principle measurements of the thermionic emission of a tungsten filament in cesium vapor. We describe a second-generation design that corrects flaws in the first gauge.
Clinical Relevance of Serum Galactose Deficient IgA1 in Patients with IgA Nephropathy
Authors: Kim Jin Sug , Hwang Hyeon Seok , Lee Sang Ho , Kim Yang Gyun , Moon Ju-Young , Kong Ji Yoon , Jeong Kyung Hwan
Published: November 2020

New biomarkers of IgA nephropathy (IgAN) are needed for non-invasive diagnosis and appropriate treatment. There is emerging evidence that galactose deficient IgA1 (Gd-IgA1) is a pivotal molecule in the pathogenesis of IgAN. However, few studies have investigated the role of Gd-IgA1 as a biomarker in IgAN. In this study, we investigated the clinical relevance of serum Gd-IgA1 levels in patients with IgAN. Two hundred and thirty biopsy-proven IgAN patients, 74 disease controls (patients with non-IgAN nephropathy), and 15 healthy controls were enrolled in this study. Levels of serum Gd-IgA1 were measured using an ELISA kit in serum samples obtained the day of renal biopsy. We compared levels of serum Gd-IgA1 according to the type of glomerular disease and analyzed the association between Gd-IgA1 levels and clinical and pathological parameters in patients with IgAN. We then divided IgAN patients into two groups according to Gd-IgA1 level and investigated the predictive value of Gd-IgA1 for progression of chronic kidney disease (CKD). Serum Gd-IgA1 levels were significantly higher in IgAN patients than disease controls and healthy controls. In patients with IgAN, serum Gd-IA1 levels were significantly correlated with estimated glomerular filtration rate, serum IgA level, and tubular atrophy/interstitial fibrosis. CKD progression was more frequent in IgAN patients with higher serum Gd-IgA1 levels than in those with lower serum Gd-IgA1 levels. Cox proportional hazard models showed that high GdIgA1 level was an independent risk factor for CKD progression after adjusting for several confounders. Our results suggest that serum Gd-IgA1 level is a useful diagnostic and prognostic marker in IgAN patients. Further studies with a larger sample size and longer follow-up duration are needed.
Disorders of Sex Development: Classification, Review, and Impact on Fertility
Authors: Acién Pedro , Acién Maribel
Published: November 2020

In this review, the elements included in both sex determination and sex differentiation are briefly analyzed, exposing the pathophysiological and clinical classification of disorders or anomalies of sex development. Anomalies in sex determination without sex ambiguity include gonadal dysgenesis, polysomies, male XX, and Klinefelter syndrome (dysgenesis and polysomies with a female phenotype; and sex reversal and Klinefelter with a male phenotype). Other infertility situations could also be included here as minor degrees of dysgenesis. Anomalies in sex determination with sex ambiguity should (usually) include testicular dysgenesis and ovotesticular disorders. Among the anomalies in sex differentiation, we include: (1) males with androgen deficiency (MAD) that correspond to those individuals whose karyotype and gonads are male (XY and testes), but the phenotype can be female due to different hormonal abnormalities. (2) females with androgen excess (FAE); these patients have ovaries and a 46,XX karyotype, but present varying degrees of external genital virilization as a result of an enzyme abnormality that affects adrenal steroid biosynthesis and leads to congenital adrenal hyperplasia; less frequently, this can be caused by iatrogenia or tumors. (3) Kallman syndrome. All of these anomalies are reviewed and analyzed herein, as well as related fertility problems.
Clinical Health Care during the COVID-19 Pandemic
Authors: Luigetti Marco , Frisullo Giovanni
Published: November 2020

In December 2019, the current outbreak of the new Coronavirus 19 (CoV) was identified in Wuhan, China, and then quickly spread over the world [...]
The Wearable VOSTARS System for Augmented Reality-Guided Surgery: Preclinical Phantom Evaluation for High-Precision Maxillofacial Tasks
Authors: Cercenelli Laura , Carbone Marina , Condino Sara , Cutolo Fabrizio , Marcelli Emanuela , Tarsitano Achille , Marchetti Claudio , Ferrari Vincenzo , Badiali Giovanni
Published: November 2020

Background: In the context of guided surgery, augmented reality (AR) represents a groundbreaking improvement. The Video and Optical See-Through Augmented Reality Surgical System (VOSTARS) is a new AR wearable head-mounted display (HMD), recently developed as an advanced navigation tool for maxillofacial and plastic surgery and other non-endoscopic surgeries. In this study, we report results of phantom tests with VOSTARS aimed to evaluate its feasibility and accuracy in performing maxillofacial surgical tasks. Methods: An early prototype of VOSTARS was used. Le Fort 1 osteotomy was selected as the experimental task to be performed under VOSTARS guidance. A dedicated set-up was prepared, including the design of a maxillofacial phantom, an ad hoc tracker anchored to the occlusal splint, and cutting templates for accuracy assessment. Both qualitative and quantitative assessments were carried out. Results: VOSTARS, used in combination with the designed maxilla tracker, showed excellent tracking robustness under operating room lighting. Accuracy tests showed that 100% of Le Fort 1 trajectories were traced with an accuracy of ±1.0 mm, and on average, 88% of the trajectory’s length was within ±0.5 mm accuracy. Conclusions: Our preliminary results suggest that the VOSTARS system can be a feasible and accurate solution for guiding maxillofacial surgical tasks, paving the way to its validation in clinical trials and for a wide spectrum of maxillofacial applications.
Therapeutic Exercise and Pain Neurophysiology Education in Female Patients with Fibromyalgia Syndrome: A Feasibility Study
Authors: Ceballos-Laita Luis , Mingo-Gómez María Teresa , Navas-Cámara Francisco , Estébanez-de-Miguel Elena , Caudevilla-Polo Santos , Verde-Rello Zoraida , Fernández-Araque Ana , Jiménez-del-Barrio Sandra
Published: November 2020

Background: We compared the effects of therapeutic exercise (TE) combined with pain neurophysiology education (PNE) to those of TE in isolation on pain intensity, general fibromyalgia impact, mechanical pain sensitivity, pain catastrophizing, psychological distress and quality of life in women with fibromyalgia syndrome (FMS). Methods: A feasibility study with a 3 month follow-up was designed. Thirty-two patients with FMS were randomly assigned to PNE + TE group (n = 16) or to TE group (n = 16). Both groups received 30 sessions of TE (3 per week), and the PNE + TE group received eight face-to-face educational sessions. The measuring instruments used were the visual analogue scale, a standard pressure algometer, the Revised Fibromyalgia Impact Questionnaire, the Pain Catastrophizing Scale, the Hospital Anxiety and Depression Scale and the Health Assessment Questionnaire. Results: The PNE + TE group showed a statistically significant decrease on pain intensity compared to TE group at short term (p = 0.015). No between-groups differences were found for mechanical pain sensitivity, general fibromyalgia impact, pain catastrophizing, psychological distress or quality of life (p > 0.05). Conclusions: The combination of PNE and TE was more effective than TE for reducing pain intensity in the short-term. No differences were found for psychological distress, pain catastrophizing and quality of life after the intervention or at 3 months of follow-up.
Trends in the Prescription of Non-Vitamin K Antagonist Oral Anticoagulants for Atrial Fibrillation: Results of the Polish Atrial Fibrillation (POL-AF) Registry
Authors: Gorczyca Iwona , Jelonek Olga , Uziębło-Życzkowska Beata , Chrapek Magdalena , Maciorowska Małgorzata , Wójcik Maciej , Błaszczyk Robert , Kapłon-Cieślicka Agnieszka , Gawałko Monika , Budnik Monika , Tokarek Tomasz , Rajtar-Salwa Renata , Bil Jacek , Wojewódzki Michał , Szpotowicz Anna , Bednarski Janusz , Bakuła-Ostalska Elwira , Tomaszuk-Kazberuk Anna , Szyszkowska Anna , Wełnicki Marcin , Mamcarz Artur , Wożakowska-Kapłon Beata
Published: November 2020

Background: Current guidelines do not suggest in which groups of patients with atrial fibrillation (AF) individual non-vitamin K antagonist oral anticoagulants (NOACs) should be used for the prevention of thromboembolic complications. The aim of this study was to evaluate the frequency of use of apixaban, dabigatran, and rivaroxaban, and attempt to identify factors predisposing their administration. Methods: The Polish Atrial Fibrillation (POL-AF) registry is a prospective, non-interventional study, including consecutive patients with AF hospitalized in ten Polish cardiology centers during the period ranging from January to December 2019. In this study, all patients were treated with NOACs. Results: Among the 2971 patients included in the analysis, 40.4% were treated with rivaroxaban, 32% with apixaban, and 27.6% with dabigatran. The mean age of the total population was 72 ± 11.5 years and 43% were female. A reduced dose of NOAC was used in 35% of patients treated with apixaban, 39.7% of patients treated with dabigatran, and 34.4% of patients treated with rivaroxaban. Independent predictors of the use of apixaban were previous bleeding (OR 2.37, CI 1.67–3.38), GFR < 60 mL/min (OR 1.38, CI 1.25–1.64), heart failure (OR 1.38, CI 1.14–1.67) and age (per 5 years) (OR 1.14, CI 1.09–1.19). GFR < 60 mL/min (OR 0.79, CI 0.66–0.95), female (OR 0.8, CI 0.67–0.96) and age (per 5 years) (OR 0.95, CI 0.91–0.99) diminished the chance of using dabigatran. Previous bleeding (OR 0.43, CI 0.28–0.64), vascular disease (OR 0.84, CI 0.70–0.99), and age (per 5 years) (OR 0.94, CI 0.90–0.97) diminished the chance of choosing rivaroxaban. Conclusions: In hospitalized patients with AF, the most frequently chosen NOAC was rivaroxaban. Apixaban was chosen more often in patients after bleeding, and in those who were advanced in years, with heart failure and impaired renal function. Impaired renal function and female gender were factors that diminished the chance of using dabigatran. Previous bleeding and vascular disease was the factor that diminished the chance of using rivaroxaban. Dabigatran and rivaroxaban have been used less frequently in elderly patients.
Intrusion Distance and Flow Discharge in Rivers during the 2011 Tohoku Tsunami
Authors: Tanaka Hitoshi , Tinh Nguyen Xuan , Hiep Nguyen Trong , Kayane Kosuke , Roh Min , Umeda Makoto , Sasaki Mikio , Kawagoe Seiki , Tsuchiya Mitsukuni
Published: November 2020

On 11 March 2011, the Great East Japan Earthquake generated huge tsunami waves. Then, tsunami propagation occurred in rivers, resulting in further expansion of the flooded area. Full data sets of tsunami characteristics such as tsunami inland and river propagation distances as well as the river geometries from all rivers within the Tohoku District were compiled and analyzed. It was found that tsunami propagation distance in rivers was about 1.2 to 4.5 times that of the inland area. There was a good correlation between propagation distance in rivers and the river bed slope. Furthermore, new empirical formulae for calculating the damping coefficient of tsunami wave height and tsunami intrusion length were successfully derived based on the current comprehensive data sets covering a wide range of river geometry and bottom slope. Moreover, tsunami-induced flow discharge was evaluated by using measured water level variation.
Pulse Ranging Method Based on Active Virtual Time Reversal in Underwater Multi-Path Channel
Authors: Zhang Zhichen , Wang Haiyan , Yao Haiyang
Published: November 2020

Aiming at improving the accuracy of pulse ranging measurement in underwater multi-path environment, this paper proposes a novel pulse ranging algorithm based on active virtual time reversal (AVTR). By using the focusing characteristics of AVTR, the received signal can be focused at the receiving end, which eliminates the negative influence of multiple pseudo-peaks. In order to extract the received signal, we propose an energy-based adaptive windowed method which preserves the signal focus peak while truncates the side peak component. Numerical simulations are provided and outfield experiments are conducted. The results demonstrate the effectiveness of the proposed method comparing with correlation-based method.
Improvement of Marine Steam Turbine Conventional Exergy Analysis by Neural Network Application
Authors: Baressi Šegota Sandi , Lorencin Ivan , Anđelić Nikola , Mrzljak Vedran , Car Zlatan
Published: November 2020

This article presented an improvement of marine steam turbine conventional exergy analysis by application of neural networks. The conventional exergy analysis requires numerous measurements in seven different turbine operating points at each load, while the intention of MLP (Multilayer Perceptron) neural network-based analysis was to investigate the possibilities for measurements reducing. At the same time, the accuracy and precision of the obtained results should be maintained. In MLP analysis, six separate models are trained. Due to a low number of instances within the data set, a 10-fold cross-validation algorithm is performed. The stated goal is achieved and the best solution suggests that MLP application enables reducing of measurements to only three turbine operating points. In the best solution, MLP model errors falling within the desired error ranges (Mean Relative Error) MRE < 2.0% and (Coefficient of Correlation) R2 > 0.95 for the whole turbine and each of its cylinders.
Photovoice—Towards Engaging and Empowering People with Intellectual Disabilities in Innovation
Authors: Wass Sofie , Safari Mugula Chris
Published: November 2020

E-health and welfare technology offer new ways to support health and social care delivery. While initiatives are made to engage disadvantaged user groups in innovation, people with intellectual disabilities tend to be excluded from design activities. This is a concern as this group can benefit from the use of assistive technology. However, it can be time-consuming and challenging to involve end-users in the design of technology. This calls for processes that are creative, empowering and that facilitate user involvement. In this study, we report and reflect on the process of using photovoice to understand user needs and to empower participants with intellectual disability in an innovation process. Nine persons with intellectual disability participated in photovoice to identify user needs connected to the design of assistive technology. The findings in our study suggest that the use of photovoice can contribute to the sharing of contextual and individual needs and an empowerment process that includes coping, self-determination and ownership. Photovoice can be a tool to reduce or remove some of the challenges that are faced when identifying user needs and is a way to strengthen the individual’s capacity to cope with the demands of participating in an innovation process.
Free Interfaces at the Tips of the Cilia in the One-Dimensional Periciliary Layer
Authors: Wuttanachamsri Kanognudge
Published: November 2020

Cilia on the surface of ciliated cells in the respiratory system are organelles that beat forward and backward to generate metachronal waves to propel mucus out of lungs. The layer that contains the cilia, coating the interior epithelial surface of the bronchi and bronchiolesis, is called the periciliary layer (PCL). With fluid nourishment, cilia can move efficiently. The fluid in this region is named the PCL fluid and is considered to be an incompressible, viscous, Newtonian fluid. We propose there to be a free boundary at the tips of cilia underlining a gas phase while the cilia are moving forward. The Brinkman equation on a macroscopic scale, in which bundles of cilia are considered rather than individuals, with the Stefan condition was used in the PCL to determine the velocity of the PCL fluid and the height/shape of the free boundary. Regarding the numerical methods, the boundary immobilization technique was applied to immobilize the moving boundaries using coordinate transformation (working with a fixed domain). A finite element method was employed to discretize the mathematical model and a finite difference approach was applied to the Stefan problem to determine the free interface. In this study, an effective stroke is assumed to start when the cilia make a 140∘ angle to the horizontal plane and the velocitiesof cilia increase until the cilia are perpendicular to the horizontal plane. Then, the velocities of the cilia decrease until the cilia make a 40∘ angle with the horizontal plane. From the numerical results, we can see that although the velocities of the cilia increase and then decrease, the free interface at the tips of the cilia continues increasing for the full forward phase. The numerical results are verified and compared with an exact solution and experimental data from the literature. Regarding the fixed boundary, the numerical results converge to the exact solution. Regarding the free interface, the numerical solutions were compared with the average height of the PCL in non-cystic fibrosis (CF) human tissues and were in excellent agreement. This research also proposes possible values of parameters in the mathematical model in order to determine the free interface. Applications of these fluid flows include animal hair, fibers and filter pads, and rice fields.
Effects of Powder Atomisation on Microstructural and Mechanical Behaviour of L-PBF Processed Steels
Authors: Abdelwahed Marawan , Casati Riccardo , Bengtsson Sven , Larsson Anna , Riccio Martina , Vedani Maurizio
Published: November 2020

In this research, steel alloys based on the Fe-Cr-Mo, Fe-Cr-Mn and Fe-Cr-Mo-Mn-Ni systems have been designed, produced by different atomisation techniques, and processed by laser powder bed fusion (L-PBF) to investigate their microstructural and mechanical behaviour. Both gas atomisation and water atomisation were considered for powder preparation. The resulting different flowability of powders, hence a different densification behaviour during processing, could be compensated by tuning the L-PBF parameters and by the application of a post treatment to improve flowability of the water atomised powders. In agreement with thermodynamic calculations, small-size oxide-based nonmetallic inclusions of the type SiO2, MnO-SiO2, Cr2O3-SiO2 were found within the steel matrix and on the fracture surfaces of the water atomised L-PBF alloys, featuring higher amounts of oxygen than the gas-atomised steels. Analyses on microstructure and hardness of the hardenable as-built steels suggested that during laser processing, the multilayer L-PBF structure undergoes an in-situ tempering treatment. Furthermore, the mechanical properties of the L-PBF steels could be widely tuned depending on the post-thermal treatment conditions.
Study of the Combined Severe Plastic Deformation Techniques Applied to Produce Contact Wire for High-Speed Railway Lines
Authors: Asfandiyarov Rashid N. , Raab Georgy I. , Aksenov Denis A.
Published: November 2020

This work considers the development and the application of combined severe plastic deformation (SPD) techniques to produce contact wire with an enhanced complex of physical, mechanical, and service properties used for high-speed railway lines. This type of processing can be used as an alternative to most conventional production methods, including rolling and drawing. The proposed technique is based on the combination of radial swaging and equal-channel angular pressing, bundled with the wire-forming process. Laboratory contact wire samples with an enhanced complex of physical, mechanical, and service properties were produced during physical experiments. The composition of processed alloy samples meets modern requirements for contact wires for high-speed railways. Ultimate tensile strength of 560 ± 20 MPa, electrical conductivity of 76 ± 2% IACS, and relative tensile elongation of 20 ± 2% are achieved through the formation of a band structure. Fragments of 300 ± 20 nm were formed inside strips with the precipitation of secondary phase particles of 20–100 nm along the fragment boundaries, mainly during the aging process.
Developmental Programming and Glucolipotoxicity: Insights on Beta Cell Inflammation and Diabetes
Authors: Cerf Marlon E.
Published: November 2020

Stimuli or insults during critical developmental transitions induce alterations in progeny anatomy, physiology, and metabolism that may be transient, sometimes reversible, but often durable, which defines programming. Glucolipotoxicity is the combined, synergistic, deleterious effect of simultaneously elevated glucose (chronic hyperglycemia) and saturated fatty acids (derived from high-fat diet overconsumption and subsequent metabolism) that are harmful to organs, micro-organs, and cells. Glucolipotoxicity induces beta cell death, dysfunction, and failure through endoplasmic reticulum and oxidative stress and inflammation. In beta cells, the misfolding of pro/insulin proteins beyond the cellular threshold triggers the unfolded protein response and endoplasmic reticulum stress. Consequentially there is incomplete and inadequate pro/insulin biosynthesis and impaired insulin secretion. Cellular stress triggers cellular inflammation, where immune cells migrate to, infiltrate, and amplify in beta cells, leading to beta cell inflammation. Endoplasmic reticulum stress reciprocally induces beta cell inflammation, whereas beta cell inflammation can self-activate and further exacerbate its inflammation. These metabolic sequelae reflect the vicious cycle of beta cell stress and inflammation in the pathophysiology of diabetes.
Structural Study of (Hydroxypropyl)Methyl Cellulose Microemulsion-Based Gels Used for Biocompatible Encapsulations
Authors: Vassiliadi Evdokia , Mitsou Evgenia , Avramiotis Spyridon , Chochos Christos , Pirolt Franz , Medebach Martin , Glatter Otto , Xenakis Aristotelis , Zoumpanioti Maria
Published: November 2020

(Hydroxypropyl)methyl cellulose (HPMC) can be used to form gels integrating a w/o microemulsion. The formulation in which a microemulsion is mixed with a hydrated HPMC matrix has been successfully used as a carrier of biocompatible ingredients. However, little is known about the structure of these systems. To elucidate this, scanning electron microscopy was used to examine the morphology and the bulk of the microemulsion-based gels (MBGs) and small-angle X-ray scattering to clarify the structure and detect any residual reverse micelles after microemulsion incorporation in the gel. Electron paramagnetic resonance spectroscopy was applied using spin probes to investigate the polar and non-polar areas of the gel. Furthermore, the enzyme-labelling technique was followed to investigate the location of an enzyme in the matrix. A structural model for HPMC matrix is proposed according to which, although a w/o microemulsion is essential to form the final gel, no microemulsion droplets can be detected after incorporation in the gel. Channels are formed by the organic solvent (oil), which are coated by surfactant molecules and a water layer in which the enzyme can be hosted.
Growth of Porous Ag@AuCu Trimetal Nanoplates Assisted by Self-Assembly
Authors: Zhang Wan-Cheng , Luoshan Meng-Dai , Wang Peng-Fei , Huang Chu-Yun , Wang Qu-Quan , Ding Si-Jing , Zhou Li
Published: November 2020

The self-assembly process of metal nanoparticles has aroused wide attention due to its low cost and simplicity. However, most of the recently reported self-assembly systems only involve two or fewer metals. Herein, we first report a successful synthesis of self-assembled Ag@AuCu trimetal nanoplates in aqueous solution. The building blocks of multibranched AuCu alloy nanocrystals were first synthesized by a chemical reduction method. The growth of Ag onto the AuCu nanocrystals in the presence of hexadecyltrimethylammonium chloride (CTAC) induces a self-assembly process and formation of Ag@AuCu trimetal nanoplates. These nanoplates with an average side length of over 2 μm show a porous morphology and a very clear boundary with the branches of the as-prepared AuCu alloy nanocrystals extending out. The shape and density of the Ag@AuCu trimetal nanoplates can be controlled by changing the reaction time and the concentration of silver nitrate. The as-assembled Ag@AuCu nanoplates are expected to have the potential for wide-ranging applications in surface-enhanced Raman scattering (SERS) and catalysis owing to their unique structures.
Feeding Ecology of Sicydium bustamantei (Greeff 1884, Gobiidae) Post-Larvae: The “Little Fish” of São Tomé Island
Authors: Baptista Vânia , Dias Ester , Cruz Joana , Branco Maria , Vieira Sara , Teodósio Maria Alexandra
Published: November 2020

The rivers of São Tomé Island are colonized by Sicydium bustamantei (Greeff 1882), an amphidromous fish that spawns in those areas. After hatching, larvae drift to the ocean with the river flow. In the marine realm, the planktonic larvae develop and migrate to freshwater as post-larvae. The migrations of post-larvae support important local fisheries at the mouth of rivers in tropical volcanic islands. Amphidromous post-larvae rely on plankton as their main source of organic matter. However, the biology and ecology of S. bustamantei in the West African islands are understudied, despite its importance for local fisheries. Thus, this study aimed to start bridging this gap by studying its feeding ecology. Our objectives were to identify the main prey of S. bustamantei post-larvae, combining gut content with stable isotope analyses. The gut contents included zooplankton (Chaetognatha, Ostracoda, and unidentified crustaceans), debris from plant and/or macroalgae-derived material, and microplastics (including microfibers). The stable isotopes analysis indicated that zooplankton and macroalgae detritus were the main sources of organic matter assimilated by this species. We also demonstrated that S. bustamantei post-larvae are omnivorous and secondary consumers. These data provide pioneering information that can be used in management plans that still need to be developed.
Antibody-Based Immunotherapeutic Strategies for COVID-19
Authors: Hussen Jamal , Kandeel Mahmoud , Hemida Maged Gomaa , Al-Mubarak Abdullah I. A.
Published: November 2020

Global efforts to contain the coronavirus disease-2019 (COVID-19) include the development of novel preventive vaccines and effective therapeutics. Passive antibody therapies using convalescent plasma, SARS-CoV-2 (Severe-Acute-Respiratory-Syndrome-Corona-Virus-2)-specific neutralizing antibodies (NAbs), and the development of monoclonal antibodies (MAbs) are among the most promising strategies for prophylaxis and treatment of SARS-CoV-2 infections. In addition, several immunomodulatory antibodies acting via several mechanisms to boost the host immune defense against SARS-CoV-2 infection as well as to avoid the harmful overreaction of the immune system are currently under clinical trial. Our main objective is to present the current most up-to-date progress in some clinical trials registered at ClinicalTrials.gov. We highlight the pros and pitfalls of several SARS-CoV-2 antibody-based immunotherapeutics.
Liposome-Based Drug Delivery Systems in Cancer Immunotherapy
Authors: Gu Zili , Da Silva Candido , Van der Maaden Koen , Ossendorp Ferry , Cruz Luis
Published: November 2020

Cancer immunotherapy has shown remarkable progress in recent years. Nanocarriers, such as liposomes, have favorable advantages with the potential to further improve cancer immunotherapy and even stronger immune responses by improving cell type-specific delivery and enhancing drug efficacy. Liposomes can offer solutions to common problems faced by several cancer immunotherapies, including the following: (1) Vaccination: Liposomes can improve the delivery of antigens and other stimulatory molecules to antigen-presenting cells or T cells; (2) Tumor normalization: Liposomes can deliver drugs selectively to the tumor microenvironment to overcome the immune-suppressive state; (3) Rewiring of tumor signaling: Liposomes can be used for the delivery of specific drugs to specific cell types to correct or modulate pathways to facilitate better anti-tumor immune responses; (4) Combinational therapy: Liposomes are ideal vehicles for the simultaneous delivery of drugs to be combined with other therapies, including chemotherapy, radiotherapy, and phototherapy. In this review, different liposomal systems specifically developed for immunomodulation in cancer are summarized and discussed.
Nanoparticle-Based Approaches towards the Treatment of Atherosclerosis
Authors: Prilepskii Artur Y. , Serov Nikita S. , Kladko Daniil V. , Vinogradov Vladimir V.
Published: November 2020

Atherosclerosis, being an inflammation-associated disease, represents a considerable healthcare problem. Its origin remains poorly understood, and at the same time, it is associated with extensive morbidity and mortality worldwide due to myocardial infarctions and strokes. Unfortunately, drugs are unable to effectively prevent plaque formation. Systemic administration of pharmaceuticals for the inhibition of plaque destabilization bears the risk of adverse effects. At present, nanoscience and, in particular, nanomedicine has made significant progress in both imaging and treatment of atherosclerosis. In this review, we focus on recent advances in this area, discussing subjects such as nanocarriers-based drug targeting principles, approaches towards the treatment of atherosclerosis, utilization of theranostic agents, and future prospects of nanoformulated therapeutics against atherosclerosis and inflammatory diseases. The focus is placed on articles published since 2015 with additional attention to research completed in 2019–2020.
EvoDevo: An Ongoing Revolution?
Authors: Amato Salvatore Ivan
Published: November 2020

Since its appearance, Evolutionary Developmental Biology (EvoDevo) has been called an emerging research program, a new paradigm, a new interdisciplinary field, or even a revolution. Behind these formulas, there is the awareness that something is changing in biology. EvoDevo is characterized by a variety of accounts and by an expanding theoretical framework. From an epistemological point of view, what is the relationship between EvoDevo and previous biological tradition? Is EvoDevo the carrier of a new message about how to conceive evolution and development? Furthermore, is it necessary to rethink the way we look at both of these processes? EvoDevo represents the attempt to synthesize two logics, that of evolution and that of development, and the way we conceive one affects the other. This synthesis is far from being fulfilled, but an adequate theory of development may represent a further step towards this achievement. In this article, an epistemological analysis of EvoDevo is presented, with particular attention paid to the relations to the Extended Evolutionary Synthesis (EES) and the Standard Evolutionary Synthesis (SET).
Nitric Oxide Overproduction by cue1 Mutants Differs on Developmental Stages and Growth Conditions
Authors: Lechón Tamara , Sanz Luis , Sánchez-Vicente Inmaculada , Lorenzo Oscar
Published: November 2020

The cue1 nitric oxide (NO) overproducer mutants are impaired in a plastid phosphoenolpyruvate/phosphate translocator, mainly expressed in Arabidopsis thaliana roots. cue1 mutants present an increased content of arginine, a precursor of NO in oxidative synthesis processes. However, the pathways of plant NO biosynthesis and signaling have not yet been fully characterized, and the role of CUE1 in these processes is not clear. Here, in an attempt to advance our knowledge regarding NO homeostasis, we performed a deep characterization of the NO production of four different cue1 alleles (cue1-1, cue1-5, cue1-6 and nox1) during seed germination, primary root elongation, and salt stress resistance. Furthermore, we analyzed the production of NO in different carbon sources to improve our understanding of the interplay between carbon metabolism and NO homeostasis. After in vivo NO imaging and spectrofluorometric quantification of the endogenous NO levels of cue1 mutants, we demonstrate that CUE1 does not directly contribute to the rapid NO synthesis during seed imbibition. Although cue1 mutants do not overproduce NO during germination and early plant development, they are able to accumulate NO after the seedling is completely established. Thus, CUE1 regulates NO homeostasis during post-germinative growth to modulate root development in response to carbon metabolism, as different sugars modify root elongation and meristem organization in cue1 mutants. Therefore, cue1 mutants are a useful tool to study the physiological effects of NO in post-germinative growth.
Maturation and Conversion of Somatic Embryos Derived from Seeds of Olive (Olea europaea L.) cv. Dahbia: Occurrence of Secondary Embryogenesis and Adventitious Bud Formation
Authors: Mazri Mouaad Amine , Naciri Rachida , Belkoura Ilham
Published: November 2020

Maturation and conversion of somatic embryos are two crucial steps that hamper the development of efficient somatic embryogenesis systems in olive. Herein, a simple and efficient protocol for the maturation and conversion of olive somatic embryos is reported. Globular somatic embryos derived from seeds of cv. Dahbia were cultured on either half-strength olive (OM) or olive cyclic embryogenesis (ECO) media, with and without plant growth regulators (PGRs). The embryos reached the cotyledonary stage in 9 weeks, but those cultured on ECO medium containing 0.1 mg·L−1 6-(dimethylallylamino)purine (2iP), 0.1 mg·L−1 6-benzyladenine (BA) and 0.05 mg·L−1 indole-3-butyric acid (IBA) exhibited the largest sizes, with an average of 4.7 mm. Somatic embryo conversion into plantlets was evaluated using different culture media (half-strength OM or one-third strength Murashige and Skoog (MS)), light conditions (light or dark) and desiccation pretreatments. The highest rate of somatic embryo conversion (45%) was observed under a 16 h photoperiod on half strength OM medium containing 0.1 mg·L−1 gibberellic acid (GA3) and 0.1 mg·L−1 1-naphthalene acetic acid (NAA). The embryos that failed to germinate showed either necrosis, cotyledon greening with no further conversion, adventitious bud formation or secondary embryogenesis. The findings of this study will be beneficial for biotechnological applications in olive.
A Meta-Analysis of Quantitative Trait Loci Associated with Multiple Disease Resistance in Rice (Oryza sativa L.)
Authors: Kumar Ilakiya Sharanee , Nadarajah Kalaivani
Published: November 2020

Rice blast, sheath blight and bacterial leaf blight are major rice diseases found worldwide. The development of resistant cultivars is generally perceived as the most effective way to combat these diseases. Plant disease resistance is a polygenic trait where a combinatorial effect of major and minor genes affects this trait. To locate the source of this trait, various quantitative trait loci (QTL) mapping studies have been performed in the past two decades. However, investigating the congruency between the reported QTL is a daunting task due to the heterogeneity amongst the QTLs studied. Hence, the aim of our study is to integrate the reported QTLs for resistance against rice blast, sheath blight and bacterial leaf blight and objectively analyze and consolidate the location of QTL clusters in the chromosomes, reducing the QTL intervals and thus identifying candidate genes within the selected meta-QTL. A total of twenty-seven studies for resistance QTLs to rice blast (8), sheath blight (15) and bacterial leaf blight (4) was compiled for QTL projection and analyses. Cumulatively, 333 QTLs associated with rice blast (114), sheath blight (151) and bacterial leaf blight (68) resistance were compiled, where 303 QTLs could be projected onto a consensus map saturated with 7633 loci. Meta-QTL analysis on 294 QTLs yielded 48 meta-QTLs, where QTLs with membership probability lower than 60% were excluded, reducing the number of QTLs within the meta-QTL to 274. Further, three meta-QTL regions (MQTL2.5, MQTL8.1 and MQTL9.1) were selected for functional analysis on the basis that MQTL2.5 harbors the highest number of QTLs; meanwhile, MQTL8.1 and MQTL9.1 have QTLs associated with all three diseases mentioned above. The functional analysis allows for determination of enriched gene ontology and resistance gene analogs (RGAs) and other defense-related genes. To summarize, MQTL2.5, MQTL8.1 and MQTL9.1 have a considerable number of R-genes that account for 10.21%, 4.08% and 6.42% of the total genes found in these meta-QTLs, respectively. Defense genes constitute around 3.70%, 8.16% and 6.42% of the total number of genes in MQTL2.5, MQTL8.1 and MQTL9.1, respectively. This frequency is higher than the total frequency of defense genes in the rice genome, which is 0.0096% (167 defense genes/17,272 total genes). The integration of the QTLs facilitates the identification of QTL hotspots for rice blast, sheath blight and bacterial blight resistance with reduced intervals, which helps to reduce linkage drag in breeding. The candidate genes within the promising regions could be utilized for improvement through genetical engineering.
Growth and Phenology of Vulpia Myuros in Comparison with Apera Spica-Venti, Alopecurus Myosuroides and Lolium Multiflorum in Monoculture and in Winter Wheat
Authors: Javaid Akhter Muhammad , Melander Bo , Mathiassen Solvejg Kopp , Labouriau Rodrigo , Vendelbo Nielsen Svend , Kudsk Per
Published: November 2020

Vulpia myuros has become an increasing weed problem in winter cereals in Northern Europe. However, the information about V. myuros and its behavior as an arable weed is limited. Field and greenhouse experiments were conducted in 2017/18 and 2018/19, at the Department of Agroecology in Flakkebjerg, Denmark to investigate the emergence, phenological development and growth characteristics of V. myuros in monoculture and in mixture with winter wheat, in comparison to Apera spica-venti, Alopecurus myosuroides and Lolium multiflorum. V. myuros emerged earlier than A. myosuroides and A. spica-venti but later than L. multiflorum. Significant differences in phenological development were recorded among the species. Overall phenology of V. myuros was more similar to that of L. multiflorum than to A. myosuroides and A. spica-venti. V. myuros started seed shedding earlier than A. spica-venti and L. multiflorum but later than A. myosuroides. V. myuros was more sensitive to winter wheat competition in terms of biomass production and fecundity than the other species. Using a target-neighborhood design, responses of V. myuros and A. spica-venti to the increasing density of winter wheat were quantified. At early growth stages “BBCH 26–29”, V. myuros was suppressed less than A. spica-venti by winter wheat, while opposite responses were seen at later growth stages “BBCH 39–47” and “BBCH 81–90”. No significant differences in fecundity characteristics were observed between the two species in response to increasing winter wheat density. The information on the behavior of V. myuros gathered by the current study can support the development of effective integrated weed management strategies for V. myuros.
Physiological Performance of Pyrus pyraster L. (Burgsd.) and Sorbus torminalis (L.) Crantz Seedlings under Drought Treatment
Authors: Paganová Viera , Hus Marek , Jureková Zuzana
Published: November 2020

In this study, seedlings of Pyrus pyraster and Sorbus torminalis were grown for 60 days in the regulated environment of a growth chamber under different water regimes. The measured indicators were the growth and distribution of mass to organs, total biomass, root to shoot mass ratio (R:S), and gas exchange parameters (gs, E, An, and water use efficiency (WUE)). The amount of total biomass was negatively affected by drought. Differences between species were confirmed only for the dry matter of the leaves. P. pyraster maintained the ratio of the mass distribution between belowground and aboveground organs in both variants of the water regime. S. torminalis created more root length for a given dry-mass under drought treatment, but its R:S was lower compared to P. pyraster. The water potential of the leaves (Ψwl) was affected by substrate saturation and interspecific differences. P. pyraster had a demonstrably higher water potential and maintained this difference even after prolonged exposure to drought. After 30 days of different water regimes, Pyrus maintained higher values of gs, An, and E in control and drought treatments, but over a longer period of drought (after 50 days), the differences between species were equalized. The changes of the leaf gas exchange for Pyrus were accompanied by a significant increase in WUE, which was most pronounced on the 40th day of the experiment. A significant and strong relationship between WUE and gs was demonstrated. The results confirmed the different physiological performances of seedlings of tree species and the different mechanisms of their response to water scarcity during drought treatment. P. pyraster presented more acclimation traits, which allowed this taxon to exhibit better performance over a longer period of water scarcity.
Self-Assembled Nanocarriers Based on Modified Chitosan for Biomedical Applications: Preparation and Characterization
Authors: Rusu Alina Gabriela , Chiriac Aurica P. , Nita Loredana Elena , Rosca Irina , Rusu Daniela , Neamtu Iordana
Published: November 2020

Protein-polysaccharide systems are of increasing interest as their combined attributes allow for fulfilling a broad range of applications in biomedical and pharmaceutical fields. In this study, the preparation of nanogels based on maleic anhydride chitosan derivatives (MAC) and bovine serum albumin (BSA) was achieved through a self-assembly process performed in aqueous phase. A series of experiments performed by varying the concentrations of MAC and BSA were conducted to find an appropriate mixing ratio for the polymer solutions leading to thermodynamically stable nanogels with the ability to encapsulate active compounds. The influence of temperature on the formation of nanogels was also studied. The consequent conformational changes were monitored using ultraviolet-visible (UV-VIS) spectrophotometry. The spectrophotometric investigations combined with diffraction light scattering (DLS) technique and zeta potential measurement results were correlated to determine the interaction mechanism and assess the self-assembling processes during nanogel formation. It was found that the hydrodynamic diameter (Dh) of the nanoparticles increased slightly at acidic pH, and the protonation of ionizable amino groups with the pH was confirmed by the zeta potential measurements. MAC/BSA nanogels also exhibited antimicrobial properties after being loaded with amoxicillin (Amox), which is an antibiotic used for the treatment of various infections. The experimental data resulting from this study provide theoretical guidance for the design and development of attractive nanocarriers for a large variety of biomedical applications.
Mechanical Properties of Fibre Reinforced Polymers under Elevated Temperatures: An Overview
Authors: Bazli Milad , Abolfazli Milad
Published: November 2020

Fibre-reinforced polymer (FRP) composite is one of the most applicable materials used in civil infrastructures, as it has been proven advantageous in terms of high strength and stiffness to weight ratio and anti-corrosion. The performance of FRP under elevated temperatures has gained significant attention among academia and industry. A comprehensive review on experimental and numerical studies investigating the mechanical performance of FRP composites subjected to elevated temperatures, ranging from ambient to fire condition, is presented in this paper. Over 100 research papers on the mechanical properties of FRP materials including tensile, compressive, flexural and shear strengths and moduli are reviewed. Although they report dispersed data, several interesting conclusions can be drawn from these studies. In general, exposure to elevated temperatures near and above the resin glass transition temperature, Tg, has detrimental effects on the mechanical characteristics of FRP materials. On the other hand, elevated temperatures below Tg can cause low levels of degradation. Discussions are made on degradation mechanisms of different FRP members. This review outlines recommendations for future works. The behaviour of FRP composites under elevated temperatures provides a comprehensive understanding based on the database presented. In addition, a foundation for determining predictive models for FRP materials exposed to elevated temperatures could be laid using the finding that this review presents.
Stem Cell Therapy for Thromboangiitis Obliterans (Buerger’s Disease)
Authors: C. Dash Biraja , Peyvandi Hassan , Duan Kaiti , Richardson Edward , U. Ndon Sifon , S. Gabrick Kyle , A. Faz Athena , A. Persing John , Dardik Alan , C. Hsia Henry
Published: November 2020

Buerger’s disease or Thromboangiitis Obliterans (TAO) is a nonatherosclerotic segmental vascular disease which affects small and medium arteries and veins in the upper and lower extremities. Based on pathological findings, TAO can be considered as a distinct form of vasculitis that is most prevalent in young male smokers. There is no definitive cure for this disease as therapeutic modalities are limited in number and efficacy. Surgical bypass has limited utility and 24% of patients will ultimately require amputation. Recently, studies have shown that therapeutic angiogenesis and immunomodulatory approaches through the delivery of stem cells to target tissues are potential options for ischemic lesion treatment. In this review, we summarize the current knowledge of TAO treatment and provide an overview of stem cell-based treatment modalities.
Violation of Bell-CHSH Inequalities through Optimal Local Filters in the Vacuum
Authors: Matsumura Akira , Nambu Yasusada
Published: November 2020

We investigate quantum correlations appearing for two-qubit detectors which are initially uncorrelated and locally coupled to a massless scalar field in a vacuum state. Under the perturbation up to the second order in the coupling, the state of the detectors can be entangled through the interaction with the scalar field but satisfies the Bell-CHSH inequality. The violation of the Bell-CHSH inequality for such an entangled state is revealed by local filtering operations. In this paper, we construct the optimal filtering operations for the qubit detectors and derive the success probability of the filtering. The success probability characterizes the reliability of revealing the violation of the Bell-CHSH inequality by the filtering operations. Through these analyses, we demonstrate a trade-off relation between the success probability and the size of parameter region showing the violation of the Bell-CHSH inequality.