bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-Benefits Analysis of Buildings Based on Different Renewal Strategies: The Emergy-Lca Approach
Authors: Cui Wenjing , Hong Jingke , Liu Guiwen , Li Kaijian , Huang Yuanyuan , Zhang Lin
Published: January 2021

Many cities have encountered challenges associated with rapid urban development, population growth and aging, in which urban renewal has become a promising option. Different renewal strategies, such as redevelopment, refurbishment and conservation, not only contributes to quality improvement and energy consumption reduction of dilapidated urban area, but also to greenhouse gas (GHG) emissions mitigation. Such integrated benefits are often termed as co-benefits. However, choosing the most co-benefits strategy to adopt requires a holistic understanding of social-economic and environmental aspects, which has been less reported in the existing literature. Under such circumstance, this article aims to shed light on the co-benefits of different renewal strategies by adopting the Emergy-Life cycle assessment method. Then, the method is applied to one case study of the refurbishment of an educational building located in Chongqing, China. Resource allocation, CO2 emissions and emergy-based indicators are calculated to assess the co-benefits during a 60-year research period, to compare the impacts of the complete demolition followed by a new one (rebuilding strategy) and the refurbishing of the existing building (refurbishment strategy). The case study shows that the annual emergy in the O&M phase of rebuilding strategy and refurbishment strategy were lower than existing building. Rebuilding and refurbishment strategies released approximately 59.1% and 80.6%, respectively, of the total CO2 emissions that would be produced by the existing building. The results reveal that substantial environmental benefits can be obtained in both the refurbishment and rebuilding strategies. On the other hand, it can be concluded that the emergy yield ratio (EYR) for the rebuilding strategy is higher than refurbishment strategy, which demonstrate the better performance of refurbishment considering that less resources are required to generate greater benefits. In addition, the value of environmental loading ratio (ELR) and emergy sustainability index (ESI) also suggests that the refurbishment strategy performs better from the perspective of the environment. Thereby, the refurbishment strategy is more suitable than the rebuilding strategy. Findings from this study can be useful to urban planners and decision-makers in choosing the most suitable strategy to improve the quality of existing buildings.
Equity of Health Services Utilisation and Expenditure among Urban and Rural Residents under Universal Health Coverage
Authors: Xu Jianqiang , Zheng Juan , Xu Lingzhong , Wu Hongtao
Published: January 2021

Worldwide countries are recognising the need for and significance of universal health coverage (UHC); however, health inequality continues to persist. This study evaluates the status and equity of residents’ demand for and utilisation of health services and expenditure by considering the three components of universal health coverage, urban-rural differences, and different income groups. Sample data from China’s Fifth Health Service Survey were analysed and the ‘five levels of income classification’ were used to classify people into income groups. This study used descriptive analysis and concentration index and concentration curve for equity evaluation. Statistically significant differences were found in the demand and utilisation of health services between urban and rural residents. Rural residents’ demand and utilisation of health services decreased with an increase in income and their health expenditure was higher than that of urban residents. Compared with middle- and high-income rural residents, middle- and lower-income rural residents faced higher hospitalisation expenses; and, compared with urban residents, equity in rural residents’ demand and utilisation of health services, and annual health and hospitalisation expenditures, were poorer. Thus, equity of health service utilisation and expenditure for urban and rural residents with different incomes remain problematic, requiring improved access and health policies.
The Impact of Intimate Partner Violence on Sexual Attitudes, Sexual Assertiveness, and Sexual Functioning in Men and Women
Authors: Sierra Juan Carlos , Arcos-Romero Ana I. , Álvarez-Muelas Ana , Cervilla Oscar
Published: January 2021

Background: Intimate Partner Violence (IPV) causes physical, sexual, or psychological harm. The association between psychosexual (sexual assertiveness, erotophilia, and attitude towards sexual fantasies) and sexual function (sexual desire, sexual excitation, erection, orgasm capacity, and sexual satisfaction), and the experience of physical and non-physical IPV was assessed. Methods: Data from 3394 (1766 women, 1628 men) heterosexual adults completed the Spanish version of the Index of Spouse Abuse, scales measuring psychosexual and sexual function, and demographic characteristics were collected. Results: For men, poorer sexual health was associated with an experience of physical abuse (F = 4.41, p < 0.001) and non-physical abuse (F = 4.35, p < 0.001). For women, poorer sexual health was associated with physical abuse (F = 13.38, p < 0.001) and non-physical abuse (F = 7.83, p < 0.001). Conclusion: The experience of physical or non-physical abuse has a negative association with psychosexual and sexual functioning in both men and women.
Readiness and Willingness to Provide Immunization Services after Pilot Vaccination Training: A Survey among Community Pharmacists Trained and Not Trained in Immunization during the COVID-19 Pandemic in Poland
Authors: Merks Piotr , Religioni Urszula , Bilmin Krzysztof , Lewicki Jedrzej , Jakubowska Marta , Waksmundzka-Walczuk Anna , Czerw Aleksandra , Barańska Agnieszka , Bogusz Joanna , Plagens-Rotman Katarzyna , Świetlik Dariusz , Drelich Ewelina , Świeczkowski Damian , Lambert Jane , Jaguszewski Miłosz , Juszczyk Grzegorz , Balkhi Bander , Vaillancourt Regis
Published: January 2021

Background: Immunization rates among the adult population in Poland are below desired targets, urging the need to expand this service in the community. During the COVID-19 pandemic, the ultimate goals for limiting the spread of the infection are vaccines against SARS-CoV-2. Pharmaceutical companies are in a race for the fastest possible way to deliver vaccines. Community pharmacists in Poland are recognised as an accessible yet underutilised group of medical professionals. Therefore, involving pharmacists in vaccinations may have beneficial results for the healthcare system. Objectives: The objectives of this study were to assess the readiness and willingness of community pharmacists following the Pharmacist Without Borders project who had either been trained or not in providing immunization services, and to identify the factors that may support the implementation of such services in Poland. Methods: This study was conducted among pharmacists between February and August 2020 in Poland. A survey was developed to determine their readiness to provide vaccination services in their pharmacies, to recognise any barriers to vaccinations, as well as the factors necessary to implement vaccination services in Polish pharmacies. Results: A total of 1777 pharmacists participated in the study, comprising 127 (7.1%) pharmacists trained in vaccinations during the Pharmacists Without Borders project and 1650 (92.9%) pharmacists not participating in the workshops. Pharmacists participating in the workshops more often indicated that providing vaccinations in community pharmacies would improve the overall vaccination rate (p = 0.0001), and that pharmacists could play an important role in advertising and promoting vaccinations (p = 0.0001). For the pharmacists not participating in the workshops, they indicated to a much greater extent possible barriers affecting the readiness to provide vaccinations in pharmacies. They most often pointed out that vaccination services would result in a significant workload increase (p = 0.0001), that pharmacies were not adapted to immunization, and that there were not enough training courses for pharmacists (p = 0.0001). Conclusion: The pharmacists working in community pharmacies indicated many advantages of vaccinations in pharmacies. This study identified barriers to the introduction of vaccinations and factors necessary to implement these services in pharmacies. The pharmacists trained during the immunization programme of the Pharmacists Without Borders project showed a greater readiness to provide immunization services.
Responses to Stress: Investigating the Role of Gender, Social Relationships, and Touch Avoidance in Italy
Authors: Passarelli Marcello , Casetta Laura , Rizzi Luca , Perrella Raffaella
Published: January 2021

Stress is a physiological response to internal and external events we call “stressors”. Response to the same daily stressors varies across individuals and seems to be higher for women. A possible explanation for this phenomenon is that women perceive sociality, relationships, and intimacy—important sources of both stress and wellbeing—differently from how men experience them. In this study, we investigate how gender, attachment, and touch avoidance predict stress responses on a sample of 335 Italians (216 females; age = 35.82 ± 14.32). Moreover, we analyze the network of relationships between these variables through multiple linear regression and exploratory network analysis techniques. The results recontextualize the role of gender in determining stress responses in terms of (lack of) confidence and touch avoidance toward family members; attitudes toward relationships seem to be the main determinants of stress responses. These results have implications for reducing stress in both clinical settings and at a social level.
Post-Traumatic Stress Symptoms in Healthcare Workers Dealing with the COVID-19 Pandemic: A Systematic Review
Authors: d’Ettorre Gabriele , Ceccarelli Giancarlo , Santinelli Letizia , Vassalini Paolo , Innocenti Giuseppe Pietro , Alessandri Francesco , Koukopoulos Alexia E. , Russo Alessandro , d’Ettorre Gabriella , Tarsitani Lorenzo
Published: January 2021

Prevention of post-traumatic stress symptoms (PTSS) in healthcare workers (HCWs) facing the current COVID-19 pandemic is a challenge worldwide as HCWs are likely to experience acute and chronic, often unpredictable, occupational stressors leading to PTSS. This review aims to analyze the literature to discover which topics have been focused on and what the latest developments are in managing the occupational risk of PTSS in HCWs during the current pandemic. For the purpose of this review, we searched for publications in MEDLINE/Pubmed using selected keywords. The articles were reviewed and categorized into one or more of the following categories based on their subject matter: risk assessment, risk management, occurrence rates. A total of 16 publications matched our inclusion criteria. The topics discussed were: “Risk Assessment”, “Occurrence Rates”, and “Risk Management”. Young age, low work experience, female gender, heavy workload, working in unsafe settings, and lack of training and social support were found to be predictors of PTSS. This review’s findings showed the need for urgent interventions aimed at protecting HCWs from the psychological impact of traumatic events related to the pandemic and leading to PTSS; healthcare policies need to consider preventive and management strategies toward PTSS, and the related psychic sequelae, in HCWs.
Influence of Breastfeeding in the Adaptation of and Absenteeism of Infants in Early Childhood Centers: A Preliminary Study
Authors: Franco-Antonio Cristina , Santano-Mogena Esperanza , Cordovilla-Guardia Sergio
Published: January 2021

Schooling of children between 0 and 3 years old involves a process of adaptation and increases their exposure to infectious diseases, which leads to school absenteeism. Breastfeeding facilitates the development of secure attachment and protects the infant against infections. This study aimed to determine whether breastfeeding facilitates the adaptation of infants between 0 and 3 years old to early childhood center and decreases school absenteeism. A cross-sectional study was carried out by collecting data through a questionnaire, which was filled out by the parents and the childcare professionals. 160 infants participated. 40% of the infants who received infant formula from birth showed dependency behaviors (inconsolable crying or do not leave the caregiver for a long time) at the time of pick-up from the center, compared with 10%, 2.7%, and 2.6% of children breastfed between 0 and 6 months, and more than 6 and 12 months, respectively (p = 0.001). The interquartile range of absenteeism days per episode was 2–3 days for infants fed infant formula versus 1–2 days for those who were breastfed for more than 12 months (p = 0.041). Breastfeeding seems to be associated with fewer dependency behaviors at the time of collection and with fewer days of absence.
Home-Based Stair Climbing as an Intervention for Disease Risk in Adult Females; A Controlled Study
Authors: Michael Elpida , White Michael J. , Eves Frank F.
Published: January 2021

Cardiovascular disease and the metabolic syndrome are major contributors to health care expenditure. Increased physical activity reduces disease risk. The study compared effects of walking up and down stairs at home with continuous, gym-based stair climbing on the disease risk factors of aerobic fitness, serum lipids, body composition, fasting blood glucose, and resting cardiovascular variables. Sedentary women (31.7 ± 1.4 years) were randomly assigned to home-based (n = 26) or gym-based (n = 24) climbing for five days.week−1 over an eight-week period. Each ascent required a 32.8-m climb, with home-based climbing matching the vertical displacement in the gym. Participants progressed from two ascents.day−1 to five ascents.day−1 in weeks 7 and 8. Relative to controls, stair climbing improved aerobic fitness (V˙O2max +1.63 mL.min−1.kg−1, 95% CI = 1.21–2.05), body composition (weight −0.99 kg, 95% CI = 1.38–0.60), and serum lipids (LDL cholesterol −0.20 mmol.L−1, 95% CI = 0.09–0.31; triglycerides −0.21 mmol.L−1, 95% CI = 0.15–0.27), with similar risk reductions for home and gym-based groups. Only the home-based protocol reduced fasting blood glucose. Discussion focuses on stair climbing bouts as time-efficient exercise and the potential benefits of a home-based intervention. Stair use at home offers a low-cost intervention for disease risk reduction to public health.
Precision Mapping of COVID-19 Vulnerable Locales by Epidemiological and Socioeconomic Risk Factors, Developed Using South Korean Data
Authors: Weinstein Bayarmagnai , da Silva Alan R. , Kouzoukas Dimitrios E. , Bose Tanima , Kim Gwang Jin , Correa Paola A. , Pondugula Santhi , Lee YoonJung , Kim Jihoo , Carpenter David O.
Published: January 2021

COVID-19 has severely impacted socioeconomically disadvantaged populations. To support pandemic control strategies, geographically weighted negative binomial regression (GWNBR) mapped COVID-19 risk related to epidemiological and socioeconomic risk factors using South Korean incidence data (20 January 2020 to 1 July 2020). We constructed COVID-19-specific socioeconomic and epidemiological themes using established social theoretical frameworks and created composite indexes through principal component analysis. The risk of COVID-19 increased with higher area morbidity, risky health behaviours, crowding, and population mobility, and with lower social distancing, healthcare access, and education. Falling COVID-19 risks and spatial shifts over three consecutive time periods reflected effective public health interventions. This study provides a globally replicable methodological framework and precision mapping for COVID-19 and future pandemics.
Exploring the Dynamic Spatio-Temporal Correlations between PM2.5 Emissions from Different Sources and Urban Expansion in Beijing-Tianjin-Hebei Region
Authors: Zhao Shen , Xu Yong
Published: January 2021

Due to rapid urbanization globally more people live in urban areas and, simultaneously, more people are exposed to the threat of environmental pollution. Taking PM2.5 emission data as the intermediate link to explore the correlation between corresponding sectors behind various PM2.5 emission sources and urban expansion in the process of urbanization, and formulating effective policies, have become major issues. In this paper, based on long temporal coverage and high-quality nighttime light data seen from the top of the atmosphere and recently compiled PM2.5 emissions data from different sources (transportation, residential and commercial, industry, energy production, deforestation and wildfire, and agriculture), we built an advanced Bayesian spatio-temporal autoregressive model and a local regression model to quantitatively analyze the correlation between PM2.5 emissions from different sources and urban expansion in the Beijing-Tianjin-Hebei region. Our results suggest that the overall urban expansion in the study area maintained gradual growth from 1995 to 2014, with the fastest growth rate during 2005 to 2010; the urban expansion maintained a significant positive correlation with PM2.5 emissions from transportation, energy production, and industry; different anti-haze policies should be designated according to respective local conditions in Beijing, Tianjin, and Hebei provinces; and during the period of rapid urban expansion (2005–2010), the spatial correlations between PM2.5 emissions from different sources and urban expansion also changed, with the biggest change coming from the PM2.5 emissions from the transport sector.
Open Community-Based Crowdsourcing Geoportal for Earth Observation Products: A Model Design and Prototype Implementation
Authors: Vahidnia Mohammad H. , Vahidi Hossein
Published: January 2021

Over the past few decades, geoportals have been considered as the key technological solutions for easy access to Earth observation (EO) products, and the implementation of spatial data infrastructure (SDI). However, less attention has been paid to developing an efficient model for crowdsourcing EO products through geoportals. To this end, a new model called the “Open Community-Based Crowdsourcing Geoportal for Earth Observation Products” (OCCGEOP) was proposed in this study. The model was developed based on the concepts of volunteered geographic information (VGI) and community-based geoportals using the latest open technological solutions. The key contribution lies in the conceptualization of the frameworks for automated publishing of standard map services such as the Web Map Service (WMS) and the Web Coverage Service (WCS) from heterogeneous EO products prepared by volunteers as well as the communication portion to request voluntary publication of the map services and giving feedback for quality assessment and assurance. To evaluate the feasibility and performance of the proposed model, a prototype implementation was carried out by conducting a pilot study in Iran. The results showed that the OCCGEOP is compatible with the priorities of the new generations of geoportals, having some unique features and promising performance.
Pairwise Coarse Registration of Indoor Point Clouds Using 2D Line Features
Authors: Li Zhen , Zhang Xiaoming , Tan Junxiang , Liu Hua
Published: January 2021

Registration is essential for terrestrial LiDAR (light detection and ranging) scanning point clouds. The registration of indoor point clouds is especially challenging due to the occlusion and self-similarity of indoor structures. This paper proposes a 4 degrees of freedom (4DOF) coarse registration method that fully takes advantage of the knowledge that the equipment is levelled or the inclination compensated for by a tilt sensor in data acquisition. The method decomposes the 4DOF registration problem into two parts: (1) horizontal alignment using ortho-projected images and (2) vertical alignment. The ortho-projected images are generated using points between the floor and ceiling, and the horizontal alignment is achieved by the matching of the source and target ortho-projected images using the 2D line features detected from them. The vertical alignment is achieved by making the height of the floor and ceiling in the source and target points equivalent. Two datasets, one with five stations and the other with 20 stations, were used to evaluate the performance of the proposed method. The experimental results showed that the proposed method achieved 80% and 63% successful registration rates (SRRs) in a simple scene and a challenging scene, respectively. The SRR in the simple scene is only lower than that of the keypoint-based four-point congruent set (K4PCS) method. The SRR in the challenging scene is better than all five comparison methods. Even though the proposed method still has some limitations, the proposed method provides an alternative to solve the indoor point cloud registration problem.
Interplay between Abscisic Acid and Gibberellins, as Related to Ethylene and Sugars, in Regulating Maturation of Non-Climacteric Fruit
Authors: Alferez Fernando , de Carvalho Deived Uilian , Boakye Daniel
Published: January 2021

In this review, we address the interaction between abscisic acid (ABA) and gibberellins (GAs) in regulating non-climacteric fruit development and maturation at the molecular level. We review the interplay of both plant growth regulators in regulating these processes in several fruit of economic importance such as grape berries, strawberry, and citrus, and show how understanding this interaction has resulted in useful agronomic management techniques. We then relate the interplay of both hormones with ethylene and other endogenous factors, such as sugar signaling. We finally review the growing knowledge related to abscisic acid, gibberellins, and the genus Citrus. We illustrate why this woody genus can be considered as an emerging model plant for understanding hormonal circuits in regulating different processes, as most of the finest work on this matter in recent years has been performed by using different Citrus species.
Mechanisms of Enhancer-Promoter Interactions in Higher Eukaryotes
Authors: Kyrchanova Olga , Georgiev Pavel
Published: January 2021

In higher eukaryotes, enhancers determine the activation of developmental gene transcription in specific cell types and stages of embryogenesis. Enhancers transform the signals produced by various transcription factors within a given cell, activating the transcription of the targeted genes. Often, developmental genes can be associated with dozens of enhancers, some of which are located at large distances from the promoters that they regulate. Currently, the mechanisms underlying specific distance interactions between enhancers and promoters remain poorly understood. This review briefly describes the properties of enhancers and discusses the mechanisms of distance interactions and potential proteins involved in this process.
Graphene-Based Materials: Biological and Biomedical Applications
Authors: Papi Massimiliano
Published: January 2021

This editorial aims to summarize the eleven scientific papers published in the Special Issue “Graphene-Based Materials: Biological and Biomedical Applications” [...]
Acute Intermittent Porphyria: An Overview of Therapy Developments and Future Perspectives Focusing on Stabilisation of HMBS and Proteostasis Regulators
Authors: Bustad Helene J. , Kallio Juha P. , Vorland Marta , Fiorentino Valeria , Sandberg Sverre , Schmitt Caroline , Aarsand Aasne K. , Martinez Aurora
Published: January 2021

Acute intermittent porphyria (AIP) is an autosomal dominant inherited disease with low clinical penetrance, caused by mutations in the hydroxymethylbilane synthase (HMBS) gene, which encodes the third enzyme in the haem biosynthesis pathway. In susceptible HMBS mutation carriers, triggering factors such as hormonal changes and commonly used drugs induce an overproduction and accumulation of toxic haem precursors in the liver. Clinically, this presents as acute attacks characterised by severe abdominal pain and a wide array of neurological and psychiatric symptoms, and, in the long-term setting, the development of primary liver cancer, hypertension and kidney failure. Treatment options are few, and therapies preventing the development of symptomatic disease and long-term complications are non-existent. Here, we provide an overview of the disorder and treatments already in use in clinical practice, in addition to other therapies under development or in the pipeline. We also introduce the pathomechanistic effects of HMBS mutations, and present and discuss emerging therapeutic options based on HMBS stabilisation and the regulation of proteostasis. These are novel mechanistic therapeutic approaches with the potential of prophylactic correction of the disease by totally or partially recovering the enzyme functionality. The present scenario appears promising for upcoming patient-tailored interventions in AIP.
Intracellular Ionic Strength Sensing Using NanoLuc
Authors: Altamash Tausif , Ahmed Wesam , Rasool Saad , Biswas Kabir H.
Published: January 2021

Intracellular ionic strength regulates myriad cellular processes that are fundamental to cellular survival and proliferation, including protein activity, aggregation, phase separation, and cell volume. It could be altered by changes in the activity of cellular signaling pathways, such as those that impact the activity of membrane-localized ion channels or by alterations in the microenvironmental osmolarity. Therefore, there is a demand for the development of sensitive tools for real-time monitoring of intracellular ionic strength. Here, we developed a bioluminescence-based intracellular ionic strength sensing strategy using the Nano Luciferase (NanoLuc) protein that has gained tremendous utility due to its high, long-lived bioluminescence output and thermal stability. Biochemical experiments using a recombinantly purified protein showed that NanoLuc bioluminescence is dependent on the ionic strength of the reaction buffer for a wide range of ionic strength conditions. Importantly, the decrease in the NanoLuc activity observed at higher ionic strengths could be reversed by decreasing the ionic strength of the reaction, thus making it suitable for sensing intracellular ionic strength alterations. Finally, we used an mNeonGreen–NanoLuc fusion protein to successfully monitor ionic strength alterations in a ratiometric manner through independent fluorescence and bioluminescence measurements in cell lysates and live cells. We envisage that the biosensing strategy developed here for detecting alterations in intracellular ionic strength will be applicable in a wide range of experiments, including high throughput cellular signaling, ion channel functional genomics, and drug discovery.
Neuroprotective Effect of Protaetia brevitarsis seulensis’ Water Extract on Trimethyltin-Induced Seizures and Hippocampal Neurodegeneration
Authors: Lee Sueun , Seo Young Hye , Song Jun Ho , Kim Wook Jin , Lee Ji Hye , Moon Byeong Cheol , Ang Mary Jasmin , Kim Sung Ho , Moon Changjong , Lee Jun , Kim Joong Sun
Published: January 2021

This study aimed to investigate whether the Protaetia brevitarsis seulensis (PB)’ water extract (PBWE) ameliorates trimethyltin (TMT)-induced seizures and hippocampal neurodegeneration. To investigate the potential neuroprotective effect of the PBWE in vitro, a lactate dehydrogenase (LDH) assay was conducted in TMT-treated primary cultures of mouse hippocampal neurons. In TMT-treated adult C57BL/6 mice, behavioral and histopathological changes were evaluated by seizure scoring and Fluoro-Jade C staining, respectively. In our in vitro assay, we observed that pretreating mice hippocampal neuron cultures with the PBWE reduced TMT-induced cytotoxicity, as indicated by the decreased LDH release. Furthermore, pretreatment with the PBWE alleviated seizures and hippocampal neurodegeneration in TMT-treated mice. The antioxidant activity of the PBWE increased in a dose-dependent manner; moreover, pretreatment with the PBWE mitigated the TMT-induced Nrf2 stimulation. In addition, six major compounds, including adenine, hypoxanthine, uridine, adenosine, inosine, and benzoic acid, were isolated from the PBWE, and among them, inosine and benzoic acid have been confirmed to have an essential antioxidative activity. In conclusion, the PBWE ameliorated TMT-induced toxicity in hippocampal neurons in both in vitro and in vivo assays, through a potential antioxidative effect. Our findings suggest that the PBWE may have pharmacotherapeutic potential in neurodegenerative diseases such as seizures or epilepsy.
Sweet Basil Has Distinct Synthases for Eugenol Biosynthesis in Glandular Trichomes and Roots with Different Regulatory Mechanisms
Authors: Reddy Vaishnavi Amarr , Li Chunhong , Nadimuthu Kumar , Tjhang Jessica Gambino , Jang In-Cheol , Rajani Sarojam
Published: January 2021

Production of a volatile phenylpropene; eugenol in sweet basil is mostly associated with peltate glandular trichomes (PGTs) found aerially. Currently only one eugenol synthase (EGS), ObEGS1 which belongs to PIP family is identified from sweet basil PGTs. Reports of the presence of eugenol in roots led us to analyse other EGSs in roots. We screened for all the PIP family reductase transcripts from the RNA-Seq data. In vivo functional characterization of all the genes in E. coli showed their ability to produce eugenol and were termed as ObEGS2-8. Among all, ObEGS1 displayed highest expression in PGTs and ObEGS4 in roots. Further, eugenol was produced only in the roots of soil-grown plants, but not in roots of aseptically-grown plants. Interestingly, eugenol production could be induced in roots of aseptically-grown plants under elicitation suggesting that eugenol production might occur as a result of environmental cues in roots. The presence of ObEGS4 transcript and protein in aseptically-grown plants indicated towards post-translational modifications (PTMs) of ObEGS4. Bioinformatics analysis showed possibility of phosphorylation in ObEGS4 which was further confirmed by in vitro experiment. Our study reveals the presence of multiple eugenol synthases in sweet basil and provides new insights into their diversity and tissue specific regulation.
Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function
Authors: Fraichard Camille , Bonnet-Serrano Fidéline , Laguillier-Morizot Christelle , Hebert-Schuster Marylise , Lai-Kuen René , Sibiude Jeanne , Fournier Thierry , Cohen Marie , Guibourdenche Jean
Published: January 2021

Protease Inhibitors (PI e.g., ritonavir (RTV) and lopinavir (LPV)) used to treat pregnant mothers infected by HIV induce prematurity and endocrine dysfunctions. The maintenance of pregnancy relies on placental hormone production (human Chorionic Gonadotrophin (hCG) and progesterone (P4)). Those functions are ensured by the villous trophoblast and are mainly regulated by the Unfolded Protein Response (UPR) pathway and mitochondria. We investigated, in vitro, if PI impair hCG and P4 production and the potential intracellular mechanisms involved. Term villous cytotrophoblast (VCT) were cultured with or without RTV or LPV from 6 to 48 h. VCT differentiation into syncytiotrophoblast (ST) was followed measuring hCG and P4 secretion. We evaluated the expression of P4 synthesis partners (Metastatic Lymph Node 64 (MLN64), cholesterol side-chain cleavage (P450SCC), Hydroxy-delta-5-Steroid Dehydrogenase and 3 Beta-and steroid delta-isomerase 1 (HSD3B1)), of mitochondrial pro-fusion factors (Mitofusin 2 (Mfn2), Optic Atrophy 1 (OPA1)) and of UPR factors (Glucose-Regulated Protein 78 (GRP78), Activating Transcription Factor 4 (ATF4), Activating Transcription Factor 6 (ATF6), spliced X-box Binding Protein 1 (sXBP1)). RTV had no significant effect on hCG and P4 secretion, whereas lopinavir significantly decreased both secretions. LPV also decreased P450SCC and HSD3B1 expression, whereas it increased Mfn2, GRP78 and sXBP1 expression in ST. RTV has no effect on the endocrine placenta. LPV impairs both villous trophoblast differentiation and P4 production. It is likely to act via mitochondrial fusion and UPR pathway activation. These trophoblastic alterations may end in decreased P4 levels in maternal circulation, inducing prematurity.
Stem Photosynthesis—A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity
Authors: Tokarz Krzysztof M. , Wesołowski Wojciech , Tokarz Barbara , Makowski Wojciech , Wysocka Anna , Jędrzejczyk Roman J. , Chrabaszcz Karolina , Malek Kamilla , Kostecka-Gugała Anna
Published: January 2021

Grass pea (Lathyrus sativus) is a leguminous plant of outstanding tolerance to abiotic stress. The aim of the presented study was to describe the mechanism of grass pea (Lathyrus sativus L.) photosynthetic apparatus acclimatisation strategies to salinity stress. The seedlings were cultivated in a hydroponic system in media containing various concentrations of NaCl (0, 50, and 100 mM), imitating none, moderate, and severe salinity, respectively, for three weeks. In order to characterise the function and structure of the photosynthetic apparatus, Chl a fluorescence, gas exchange measurements, proteome analysis, and Fourier-transform infrared spectroscopy (FT-IR) analysis were done inter alia. Significant differences in the response of the leaf and stem photosynthetic apparatus to severe salt stress were observed. Leaves became the place of harmful ion (Na+) accumulation, and the efficiency of their carboxylation decreased sharply. In turn, in stems, the reconstruction of the photosynthetic apparatus (antenna and photosystem complexes) activated alternative electron transport pathways, leading to effective ATP synthesis, which is required for the efficient translocation of Na+ to leaves. These changes enabled efficient stem carboxylation and made them the main source of assimilates. The observed changes indicate the high plasticity of grass pea photosynthetic apparatus, providing an effective mechanism of tolerance to salinity stress.
From Rheumatoid Factor to Anti-Citrullinated Protein Antibodies and Anti-Carbamylated Protein Antibodies for Diagnosis and Prognosis Prediction in Patients with Rheumatoid Arthritis
Authors: Wu Chao-Yi , Yang Huang-Yu , Luo Shue-Fen , Lai Jenn-Haung
Published: January 2021

Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease mainly involving synovial inflammation and articular bone destruction. RA is a heterogeneous disease with diverse clinical presentations, prognoses and therapeutic responses. Following the first discovery of rheumatoid factors (RFs) 80 years ago, the identification of both anti-citrullinated protein antibodies (ACPAs) and anti-carbamylated protein antibodies (anti-CarP Abs) has greatly facilitated approaches toward RA, especially in the fields of early diagnosis and prognosis prediction of the disease. Although these antibodies share many common features and can function synergistically to promote disease progression, they differ mechanistically and have unique clinical relevance. Specifically, these three RA associating auto-antibodies (autoAbs) all precede the development of RA by years. However, while the current evidence suggests a synergic effect of RF and ACPA in predicting the development of RA and an erosive phenotype, controversies exist regarding the additive value of anti-CarP Abs. In the present review, we critically summarize the characteristics of these autoantibodies and focus on their distinct clinical applications in the early identification, clinical manifestations and prognosis prediction of RA. With the advancement of treatment options in the era of biologics, we also discuss the relevance of these autoantibodies in association with RA patient response to therapy.
Molecular Properties of Bare and Microhydrated Vitamin B5–Calcium Complexes
Authors: Corinti Davide , Chiavarino Barbara , Scuderi Debora , Fraschetti Caterina , Filippi Antonello , Fornarini Simonetta , Crestoni Maria Elisa
Published: January 2021

Pantothenic acid, also called vitamin B5, is an essential nutrient involved in several metabolic pathways. It shows a characteristic preference for interacting with Ca(II) ions, which are abundant in the extracellular media and act as secondary mediators in the activation of numerous biological functions. The bare deprotonated form of pantothenic acid, [panto-H]−, its complex with Ca(II) ion, [Ca(panto-H)]+, and singly charged micro-hydrated calcium pantothenate [Ca(panto-H)(H2O)]+ adduct have been obtained in the gas phase by electrospray ionization and assayed by mass spectrometry and IR multiple photon dissociation spectroscopy in the fingerprint spectral range. Quantum chemical calculations at the B3LYP(-D3) and MP2 levels of theory were performed to simulate geometries, thermochemical data, and linear absorption spectra of low-lying isomers, allowing us to assign the experimental absorptions to particular structural motifs. Pantothenate was found to exist in the gas phase as a single isomeric form showing deprotonation on the carboxylic moiety. On the contrary, free and monohydrated calcium complexes of deprotonated pantothenic acid both present at least two isomers participating in the gas-phase population, sharing the deprotonation of pantothenate on the carboxylic group and either a fourfold or fivefold coordination with calcium, thus justifying the strong affinity of pantothenate for the metal.
PKC Regulates YAP Expression through Alternative Splicing of YAP 3′UTR Pre-mRNA by hnRNP F
Authors: Chu Wing-Keung , Hung Li-Man , Hou Chun-Wei , Chen Jan-Kan
Published: January 2021

The Yes-associated protein (YAP) is a transcriptional co-activator that plays critical roles in organ development and tumorigenesis, and is verified to be inhibited by the Hippo signaling pathway. In the present study, we show that the YAP 3′UTR is alternatively spliced to generate a novel 950 bp 3′UTR mRNA from the full length 3′UTR region (3483 bp) in human cancer cells. The ratio of full length 3′UTR YAP mRNA to alternatively spliced 3′UTR YAP mRNA is up-regulated by exposure of the cells to PKC inhibitor chelerythrine chloride. Further study using luciferase reporter assay showed that the expression of the alternatively spliced 3′UTR mRNA is much lower compared with the full length 3′UTR mRNA, suggesting that alternatively spliced 3′UTR YAP mRNA may have a shorter half-life than full length 3′UTR mRNA. Interestingly, PKC represses YAP 3′UTR–mediated mRNA stability is dependent on a splicing factor, hnRNP F. Activation of PKC induces nuclear translocation of cytosolic hnRNP F. Ectopic expression of hnRNP F enhances YAP 3′UTR splicing. Our results suggest that hnRNP F regulates YAP 3′UTR-mediated mRNA stability in an alternative splicing-dependent manner, and PKC regulated YAP expression is dependent on nuclear translocation of hnRNP F in human cancer cell lines.
β-Cyclodextrin/Triclosan Complex-Grafted Methacrylated Glycol Chitosan Hydorgel by Photocrosslinking via Visible Light Irradiation for a Tissue Bio-Adhesive
Authors: Moon Young Jae , Yoon Sun-Jung , Koo Jeung-Hyun , Yoon Yihyun , Byun Hye Jun , Kim Hyeon Soo , Khang Gilson , Chun Heung Jae , Yang Dae Hyeok
Published: January 2021

Accelerating wound healing with minimized bacterial infection has become a topic of interest in the development of the new generation of tissue bio-adhesives. In this study, we fabricated a hydrogel system (MGC-g-CD-ic-TCS) consisting of triclosan (TCS)-complexed beta-cyclodextrin (β-CD)-conjugated methacrylated glycol chitosan (MGC) as an antibacterial tissue adhesive. Proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) results showed the inclusion complex formation between MGC-g-CD and TCS. The increase of storage modulus (G’) of MGC-g-CD-ic-TCS after visible light irradiation for 200 s indicated its hydrogelation. The swollen hydrogel in aqueous solution resulted in two release behaviors of an initial burst and sustained release. Importantly, in vitro and in vivo results indicated that MGC-g-CD-ic-TCS inhibited bacterial infection and improved wound healing, suggesting its high potential application as an antibacterial tissue bio-adhesive.
A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification
Authors: Gozdziewicz Tomasz K , Maciejewska Anna , Tsybulska Alona , Lugowski Czeslaw , Lukasiewicz Jolanta
Published: January 2021

Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria. It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide − endotoxin (ECALPS), and cyclic form (ECACYC). ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria. A supernatant obtained after LPS ultracentrifugation was reported as a source for ECA isolation, but it has never been assessed for detailed composition besides ECACYC. We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations. Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants. We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation. Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units. Described results were common for all selected strains. The origin of linear ECA is discussed against the current knowledge about ECAPG and ECALPS.
Odorants of Capsicum spp. Dried Fruits as Candidate Attractants for Lasioderma serricorne F. (Coleoptera: Anobiidae)
Authors: Guarino Salvatore , Basile Sara , Arif Mokhtar Abdulsattar , Manachini Barbara , Peri Ezio
Published: January 2021

The cigarette beetle, Lasioderma serricorne F. (Coleoptera: Anobiidae) is an important food storage pest affecting the tobacco industry and is increasingly impacting museums and herbaria. Monitoring methods make use of pheromone traps which can be implemented using chili fruit powder. The objective of this study was to assess the response of L. serricorne to the volatile organic compounds (VOCs) from different chili powders in order to identify the main semiochemicals involved in this attraction. Volatiles emitted by Capsicum annuum, C. frutescens, and C. chinense dried fruit powders were tested in an olfactometer and collected and analyzed using SPME and GC-MS. Results indicated that C. annuum and C. frutescens VOCs elicit attraction toward L. serricorne adults in olfactometer, while C. chinense VOCs elicit no attraction. Chemicals analysis showed a higher presence of polar compounds in the VOCs of C. annuum and C. frutescens compared to C. chinense, with α-ionone and β-ionone being more abundant in the attractive species. Further olfactometer bioassays indicated that both α-ionone and β-ionone elicit attraction, suggesting that these compounds are candidates as synergistic attractants in pheromone monitoring traps for L. serricorne.
Evaluation of Slug Refuge Traps in a Soybean Reduced-Tillage Cover Crop System
Authors: Raudenbush Amy L. , Pekarcik Adrian J. , Haden Van R. , Tilmon Kelley J.
Published: January 2021

As more farmers adopt no- or reduced-tillage and/or cover crop land management practices, slugs have become more frequent pests of field crops, including soybean. Monitoring slugs visually is difficult because they are nocturnal, so several trapping methods have been developed, though comparisons of trap types are rare. The objective of this study was to compare trapping efficiency of two types of slug refuge traps in reduced-tillage soybeans following cover crop termination. We tested a traditional shingle trap and a modified shingle trap with a water-filled pitfall trap beneath it. Traps were deployed in 24 pairs in 2018 and 2019 in experimental soybean plots. We counted slug captures weekly over a 5-week time period each year. In 2018, we counted the total number of slugs under each trap type. In 2019, counts were categorized into specific trap components (shingle vs. in/on/under the pitfall). Temperature was also recorded in 2019. The modified shingle traps captured significantly more slugs than the traditional shingle traps, mainly due to the pitfall component. This trend was most pronounced as slug density decreased, suggesting that the modified shingle trap is a more sensitive sampling tool which may be particularly valuable when used for research purposes.
New Genera and Species of the Family Throscidae (Coleoptera: Elateroidea) in Mid-Cretaceous Burmese Amber
Authors: Li Yan-Da , Huang Di-Ying , Cai Chen-Yang
Published: January 2021

Captopus depressicepsgen. et sp. nov., Electrothroscus yanpingaegen. et sp. nov. and Pseudopactopus robustusgen. et sp. nov. are reported from the mid-Cretaceous Burmese amber. These new findings greatly extend the Mesozoic diversity of Throscidae, which implies a high degree of morphological disparity for this family in the Cretaceous.
Physiological Age- and Sex-Related Profiles for Local (Aortic) and Regional (Carotid-Femoral, Carotid-Radial) Pulse Wave Velocity and Center-to-Periphery Stiffness Gradient, with and without Blood Pressure Adjustments: Reference Intervals and Agreement between Methods in Healthy Subjects (3–84 Years)
Authors: Bia Daniel , Zócalo Yanina
Published: January 2021

In addition to being a marker of cardiovascular (CV) aging, aortic stiffening has been shown to be independently associated with increased CV risk (directly and/or indirectly due to stiffness-gradient attenuation). Arterial stiffness determines the rate at which the pulse pressure wave propagates (i.e., pulse wave velocity, PWV). Thus, propagated PWV (i.e., the distance between pressure-wave recording sites divided by the pulse transit time) was proposed as an arterial stiffness index. Presently, aortic PWV is considered a gold-standard for non-invasive stiffness evaluation. The limitations ascribed to PWV have hampered its use in clinical practice. To overcome the limitations, different approaches and parameters have been proposed (e.g., local PWV obtained by wave separation and pulse wave analysis). In turn, it has been proposed to determine PWV considering blood pressure (BP) levels (β-PWV), so as to evaluate intrinsic arterial stiffness. It is unknown whether the different approaches used to assess PWV or β-PWV are equivalent and there are few data regarding age- and sex-related reference intervals (RIs) for regional and local PWV, β-PWV and PWV ratio. Aims: (1) to evaluate agreement between data from different stiffness indexes, (2) to determine the need for sex-specific RIs, and (3) to define RIs for PWV, β-PWV and PWV ratio in a cohort of healthy children, adolescents and adults. Methods: 3619 subjects (3–90 y) were included, 1289 were healthy and non-exposed to CV risk factors. Carotid-femoral (cfPWV) and carotid-radial (crPWV) PWV were measured (SphygmoCor System (SCOR)) and PWV ratio (cfPWV/crPWV) was quantified. Local aortic PWV was obtained directly from carotid waves (aoPWV-Carotid; SCOR) and indirectly (generalized transfer function use) from radial (aoPWV-Radial; SCOR) and brachial (aoPWV-Brachial; Mobil-O-Graph system (MOG)) recordings. β-PWV was assessed by means of cardio-ankle brachial (CAVI) and BP-corrected CAVI (CAVIo) indexes. Analyses were done before and after adjustment for BP. Data agreement was analyzed (correlation, Bland-Altman). Mean and standard deviation (age- and sex-related) equations were obtained for PWV parameters (regression methods based on fractional polynomials). Results: The methods and parameters used to assess aortic stiffness showed different association levels. Stiffness data were not equivalent but showed systematic and proportional errors. The need for sex-specific RIs depended on the parameter and/or age considered. RIs were defined for all the studied parameters. The study provides the largest data set related to agreement and RIs for stiffness parameters obtained in a single population.