bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Study on Mechanical Property of Concrete Based on Seawater and Sea Sand
Authors: Li Ying Tao , Zhou Ling , Jiang Mao , Zhang Yu , Shao Jun
Published: March 2013

In this paper, the mechanical property experiments of concrete based on the seawater and sea sand have been carried in different raw materials preparation and different conservation environments. The results show that the early strength and late strength of concrete based on seawater and sea sand are better than concrete based on freshwater and sand. There is no significant strength decreased for concrete based on seawater and sea sand under accelerated alternating wet and dry conditions. For concrete based on seawater and sea sand mixed with admixture, the downward trend of late strength is significantly delayed, the late strength of concrete based on the seawater and sea sand mixed with slag gets the most obvious growth trend, while the late strength of seawater and sea sand concrete mixed with fly ash gets the largest increment.
Potential Species and Character of Wild Diesel Plant in Tianjin
Authors: Yang Jing Hui , Liu Yan Jun , Li Jian Ke , Huang Jun Xun , Zhang Wei Yu , Li Shuang Yue
Published: March 2013

In order to reveal the potentials of unknown and lessknown herbaceous wild plant (potential diesel plant) from saline-alkali wasteland for development biodiesel production, seed and plant samples from 33 species were collected and plant oil content, seed oil content, seed yield per plants and saline-alkali tolerance of the plants were analyzed. The result show that oil content in plants ranged from 1.31-15.01%. Euphorbia heyneana (15.01%) had the highest oil content followed by Ricinus communis (13.9%), Cirsium setosum (12.5%), Euphorbia nutans (11.02%), Cirsiu japonicum (9.27%). About fifty percent species were found have more seed oil content within the range of 21 to 48.5%. Maximum of 48.5% was observed in one wild species of Ricinus communis followed by Euphorbia esula (34.2%), Euphorbia nutans (29.3%), Xanthium sibiricum (28.5%), Euphorbia humifusa (28.1%), Euphorbia heyneana (24.1%), Capsella bursa-pastoris (24%), Suaeda glauca (23.6%), Artemisia argyi (23.5%), Lepidium apetalum (23%). Highest level of seed field per plant was observed in Ricinus communis, Glycine soja and the higher level of seed field was in Humulus scandens, Sonchus oleraceus, Gynura crepidioides, Artemisia argyi, Abutilon theophrasti, Cirsiu japonicum, Inula japonica, Comnyza canadensis. 14 species grew in moderate saline-alkali soil and only one species (Suaeda glauca) was in severe saline-alkali soil. Comprehensive analysis show that most potential herbaceous diesel plants are Ricinus communis, Euphorbia esula, Glycine soja, Gynura crepidioides, Cirsiu japonicum and Artemisia argyi, based on 4 values of oil content in plants, and seed, seed yield per plants and saline-alkali tolerance.
Research in Optimization of Inner-Grooved Copper Tubes Forming
Authors: Liu Jin Song , Wang Xin Peng
Published: March 2013

By using the method of orthogonal design based on the inner-grooved copper tubes (φ7.00×0.24), the influence in the different forming conditions was analyzed. The forming laws and optimization forming process parameters were also obtained during the experiment. The results show that the motor and drawing speed should be controlled about 23000r/min and 15m/min respectively, and the spinning position should be located at about 1.5mm away from the center of the inner-grooved core head.
Analysis of Synonymous Codon Usage Bias in D15 Gene Encoded Surface Antigen of Riemerella Anatipestifer
Authors: Feng Bin , Zhu De Kang , Wang Xiao Jia , Cheng An Chun , Wang Ming Shu
Published: March 2013

In order to provide a basis for understanding the evolutionary relationship and pathogenesis of Riemerella anatipestifer and selecting a appropriate host expression systems to improve the expression of target gene in vivo and in vitro, we identified the codon bias in the newly confirmed D15 gene of Riemerella anatipestifer ATCC 11845 strain and performed comparative analysis of the codon usage bias between D15 gene in R. anatipestifer and the other 10 referenced Flavobacteriaceaes by a series of online bioinformatics softwares. The results revealed that the synonymous codons with A and T at the third codon position had widely usage in the codon of D15 gene of R. anatipestifer. In addition, there were 70 rare codons in the ORF of the D15 of R. anatipestifer, and 32 codons showing distinct usage differences between R. anatipestifer and E. coli, 30 codons between R. anatipestifer and Homo sapiens, 16 codons between R. anatipestifer and yeast, indicated the yeast expression system may be more suitable for the expression of R. anatipestifer genes. The extent of codon usage bias in the D15 gene in R. anatipestifer was highly correlated with the gene expression level, therefore the results may provide useful information for gene classiﬁcation and functional studies.
Experimental Research on the Mineral Processing of a Certain Refractory Limonite Ore in Xinjiang
Authors: Jia Qing Mei , Li Feng Jiu , Li Ying
Published: March 2013

According to the ore nature of limonite in Xinjiang，use the technological process of one stage selectrifine high magnetic separation—reverse flotation to the limonite. the result show that at the fineness ore of -200order 60.0%, employ the closed-circuit process ,Limonite concentrates aining above 52.24％ Fe could be obtained at a productive rate of 54.04％ and a recovery of Fe about 67.03％ losed circuit tests, and the numble of finally tailing is 47.76%,29.08%and 32.97%.
Bioinformatics Analysis and Characteristics of UL17 Protein Encoded by the UL17 Gene from Duck Enteritis Virus
Authors: Wen Ting , Cheng An Chun , Wang Ming Shu
Published: March 2013

Previous studies indicate that the UL17 protein and its homology play similar roles in viral DNA cleavage and packaging of herpesviruses. However, there is no report on the UL17 gene product of DEV. Here in this article we used various bioinformatics softwares and online web servers to analyze the basic properties of the DEV UL17 protein. The results showed that the DEV UL17 protein was 75.94298 kDa, consisting of 684 amino acids. It had multiple motif sites, phosphorylation sites and antigenic epitopes. There were no predicted signal peptide sites or transmembrane regions in DEV UL17 protein. The results of secondary structure and teriary structure were exhibited in the pictures in this article. These properties of the DEV UL17 protein provide a prerequisite for further functional analysis of this gene.
Characterization of Codon Usage Bias in the RA Ragb/SusD Gene
Authors: Yang Si Si , Zhu De Kang , Wang Xiao Jia , Cheng An Chun , Wang Ming Shu
Published: March 2013

The analysis on codon usage bias of Riemerella anatipestifer (RA) RagB/SusD gene (GenBank accession No. NC_017045.1) may improve our understanding of the evolution and pathogenesis of RA and provide a basis for understanding the relevant mechanism for biased usage of synonymous codons and for selecting appropriate expression systems to improve the expression of target genes. In this study, the synonymous codon usage in the RagB/SusD gene of RA and 19 reference bacteroidetes have been investigated. The results showed that codon usage bias in the RagB/SusD gene was strong bias towards the synonymous codons with A and T at the third codon position. A high level of diversity in codon usage bias existed, and the effective number of codons used in a gene plot revealed that the genetic heterogeneity in RagB/SusD gene of bacteroidetes was constrained by the G + C content. The codon adaptation index (CAI), effective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the RagB/SusD gene of bacteroidetes, and this synonymous bias was correlated with host evolution. The phylogentic analysis suggested that RagB/SusD was evolutionarily closer to Ornithobacterium rhinotracheale and that there was no significant deviation in codon usage in different bacteroidetes. There are 25 codons showing distinct usage differences between RA RagB/SusD and E. coli, 30 between RA RagB/SusD and Homo sapiens, 26 codons between RA RagB/SusD and yeast. Therefore the yeast and E. coli expression system may be suitable for the expression of RA RagB/SusD gene if some codons could be optimized.
Characterization of Synonymous Codon Usage Bias in the Riemerella Anatipestifer OmpA/MotB Gene
Authors: Xu Pan , Cheng An Chun , Wang Ming Shu , Zhu De Kang , Wang Xiao Jia
Published: March 2013

The analysis on codon usage bias of OmpA/MotB gene of Riemerella anatipestifer (RA) may provide a basis for understanding the evolution and pathogenesis of RA and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In our study, a comparative analysis of the codon usage bias in the newly discovered RA OmpA/MotB gene and the OmpA/MotB gene of 20 reference flavobacteriaceae was performed. The results of the codon adaptation indes (CAI), effective number of codon (ENC), and GC3s values indicated that synonymous codon usage bias in the OmpA/MotB gene of flavobacteriaceae. The results showed that codon usage bias in the RA OmpA/MotB gene was strong bias towards the synonymous codons with A and T at the third codon position. A high level of diversity in codon usage bias existed, and the effective number of codons used in a gene plot revealed that the G+C compositional constraint is the main factor that determines the codon usage bias in OmpA/MotB gene of flavobacteriaceae. Comparison of the codon usage in the OmpA/MotB gene of different organisms revealed that there were 31 codons showing distinct codon usage differences between the RA and E. coli, 41 between the RA and humans, but 29 between the RA and yeast. Therefore the yeast expression system may be more suitable for the expression of RA OmpA/MotB gene. These results may improve our understanding of the evolution, pathogenesis and functional studies of RA, as well as contribute significantly to the area of flavobacteriaceae research.
Characterization of Synonymous Codon Usage Bias in the UL1 Gene of Duck Plague Virus
Authors: Zuo Ling Jie , Cheng An Chun , Wang Ming Shu
Published: March 2013

In this study, we calculated the codon usage bias in DPV CHv UL1 gene and performed a comparative analysis of synonymous codon patterns in UL1 of DPV CHv strain and other 19 reference herpesviruses. The results revealed that the synonymous codons with A and T at the third codon positon have widely usage in the codon of UL1 gene of DPV CHv. G + C compositional constraint was the main factor that determined the codon usage bias in UL1 gene. In addition, the codon usage bias of DPV CHv UL1 gene was compared with those of E. coli, yeast and human. There are 25 codons showing distinct usage differences between DPV and E. coli, 26 codons between DPV and yeast, and 21 codons between DPV and human. Therefore, the Human expression system is more suitable for heterologous expression of the DPV UL1 gene.
Combustion and Emission Characteristics of YC6A220C Diesel Engine Fueled with Bio-Diesel
Authors: Miao Yong Chao , Wang Bing Hui , Chen Kai
Published: March 2013

We measured the different performance of bio-diesel,which was used to compare with 0#diesel ,on the diesel engine YC6A220C without any adjustments. The result is that under the five different work condition,using bio-diesel has a good impact on protecting environment and reducing emission.Compared with 0#diesel,bio-diesel oil consumption rose by3.48%,but NOx and PM2.5 dramatically dropped by 15.20% and 3.67%.
Effect of Exciting Conditions on the Delayed Luminescence of Soybeans
Authors: Guo Jian Jun , Bai Hua , Lin Lie , Tang Guo Qing
Published: March 2013

The effects of different lighting energy and different radiant power on the properties of delayed luminescence of beans were discussed. It is showed when lighting energy is from 2 to 25mJ, initial intensity A changes very much from 1200 to 8600. When lighting energy is from 200 to 600 mJ, initial intensity A has little change. Initial intensity A is in a linear relation with radiant power.
Effects of Different Intensity Walking on Serum Nitric Oxide for the Elderly Men
Authors: Ren Ke Xin
Published: March 2013

Objective: In order to study the effect of different intensity exercise on serum nitric oxide and nitric oxide synthase of elderly men, and look for the most appropriate exercise intensity for them which can prevent cardiovascular disease. Methods: 36 men who are 55 to 62 years old, were randomly divided into the low intensity group(L group,n=12), the moderate-intensity group(M group,n=12) and the high-intensity group(H group,n=12). During the period for testing, they walked for 12 weeks, 4 times per week, 45 minutes for each time,target heart rate is 100 ~110 beats/min(L group), 110 ~120 beats/min(M group), 120~ 130 beats/min(H group). With an over-night fast, all the subject venous blood samples were drawn by 4ml before and after the exercise. The blood samples were centrifuged and separated immediately, then serum nitric oxide content, TNOS activity, cNOS activity, iNOS activity were measured.Results:In low-intensity group, serum NO content, TNOS activity, cNOS activity were significantly increased than pre-exercise (P<0.05); in moderate-intensity group,serum NO content,TNOS activity,cNOS activity were very significantly increased than pre-exercise (P <0.01);the indicators of high-intensity exercise group did not change significantly. Conclusion: we recommend that a more appropriate exercise intensity is 100-120 beats /min for elderly during fitness exercise, and especially 110-120 beats/min. Such intensity exercise can increase NO content in vivo, thus effectively prevent the occurrence of cardiovascular disease.
Identification of Volatile Components in Grafted Liquidambar orientalis Mill.
Authors: Ma Li , Li Wei Lin , Qin Lu Ping , Yao Shun
Published: March 2013

The volitile oil was extracted with n-hexane and analyzed with GC-MS from the leaf, bark and heartwood of grafted Liquidambar orientalis Mill., and 22, 16, 18 compounds were identified respectively, of which γ-sitosterol was found in leaf, bark and heartwood, and 1-methoxy-3-(2-hydroxyethyl) was found in leaf and bark. Vitamin E was enriched in bark while dibutyl phthalate was abundant in heartwood.
SNP Detection by Using Affymetix Barley 1 Array in Barley
Authors: Guo Pei Guo , Li Rong Hua , Salvato Nicoló , Filosi Michele , Xia Yan Shi
Published: March 2013

Nowadays the research for discovering SNPs is becoming one of the most important topics to understand the differences between genotypes of the same species. The objective of this study is to discover the SNPs using oligonucleotide microarrays in barley. Two methods are used for the prediction step: one is Robustified Projection Pursuit (RPP) method and another is based on a statistic test between probes of a probeset belonging different genotypes. Following the procedure described above, 124 single feature polymorphisms (SFPs), which can be used for identification of SNPs or insertion/deletions (INDELs), were detected among all genotypes. Sequencing of putative SFP-containing amplicons from the EST region in the genome yielded a validation rate of 85%, indicating the Affymetrix Barley 1 array could provide a satisfactory platform for identification of SNP in barley.
Thermal Alkaline Method to Prepare Geotrichuam Candidum Protein Foaming Agent
Authors: Zheng Jie Jie , Huo Ji Chuan , Lei Hong , Ai Wei
Published: March 2013

Geotrichum Candidum strain numbered 2.0498 was used as raw material. The Geotrichum Candidum thallus were collected through inoculation and shaking cultivation. Thermal alkaline method was applied for hydrolyzing Geotrichum Candidum mycoprotein to prepare protein foaming agent. The effect of bacteria concentration, Ca(OH)2mass concentration, hydrolysis temperature and hydrolysis time on foam expansion were studied. The optimization strategy was single factor test and subsequent response surface analysis based on Box-Behnken design. The results showed that the optimized condition were a bacteria concentration of 5.4%(w/v) and a Ca(OH)2mass concentration of 1.5%(w/v), at a hydrolysis temperature of 95°C for 4.1 h. Under these optimal conditions, the foam expansion was 28.1. Compared with the model theory prediction, the relative error was 0.46%. At the same time, a multiple quadratic regression equation between Geotrichum Candidum mycoprotein foam expansion and impact factors was setted up and it played an important role in predicting the foam expansion.
Assessment of Tobacco Specific Nitrosamines in Tobacco Genotypes
Authors: Guo Pei Guo , Xia Yan Shi , Li Rong Hua , Lü Yong Hua , Qiu Miao Wen , Zhao Wei Chai , Yu Yi Wen
Published: March 2013

The tobacco-specific nitrosamines (TSNAs) are one of the most important groups of carcinoigens in tobacco leaf, knowing the difference of TSNAs contents in tobacco genotypes should be beneficial for breeding low TSNAs cultivars which could decrease the harmfulness to human health in tobacco products. For this purpose, 8 genotypes (include burley, sun-cured and flue-cured tobacco) were selected as materials, the TSNAs contents of uncured and cured leaves were assessed with LC-MS/MS. The results showed that TSNAs contents were significantly increased after curing in all genotypes. The mean TSNAs contents of leaves before and after curing in burley were the highest among three types of tobacco, and the contents in sun-cured tobacco were the lowest. Coefficient of variation (CV) of TSNAs among genotypes in burley was higher than that in flue-cured and sun-cured tobacco, and the CV in sun-cured tobacco was the lowest. The positive correlation was observed in NNN, NAT and total TSNAs contents between uncured and cured leaves in three types of tobacco. Therefore, the TSNAs contents in uncured leaves could be considered as evaluation index to screen tobacco cultivars with the low TSNAs contents.
Comparison of Antioxidant Activity in Five Fruit Wine of Guangxi in China
Authors: Yuan Zhu Lian
Published: March 2013

With reference to VC, for the first time, this thesis studied the antioxidant activity in five fruit wine of Guangxi by five different systems（NO2－, •OH, O2-•,DPPH• and reduction ability）and the scoring method. Study results indicated: Firstly, five fruit wine had strong antioxidant ability in vitro except the Rosa laevigata michx wine had no scavenging effect on O2-•. Especially Fructus phyllanthus wine and the Rosa laevigata michx wine had scavenging effect on •OH as the same as VC and more reduction ability than VC. What’s more, Sour plum wine had more scavenging effect on O2-•than VC; Secondly, by scoring method, the following was the antioxidant activity in order: Fructus rhodomyrti wine
The Protoplast Preparation of Two Strains of Saccharamyces cerevisae
Authors: Zhao Chun Hai , Liu Zhi Gang
Published: March 2013

This paper researched on the condition of protoplast preparation for two strains isolated by myself, selected the different of time、concentration of zymolase and temperature, in the end ,the proper condition was the 0.2mg/ml of zymolase, 30°C, the time of 40 minutes, the ratio of regeneration was 68.2%.
Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus
Authors: Lu An Gen , Yang Ze Xi , Wang Fei , Xu Lang , Deng Wen Ying , Meng Jian Zong , Huang Zi Qi , Qi Xiang Hui
Published: March 2013

Ethanol produced from hexose and pentose sugars hydrolysated by lignocellulose is an environment-friendly alternative to fossil fuels. Xylose isomerase is the major rate-limiting enzyme in the ethanol synthesis biologically pathway of xylose fermentation. In present study, xylA gene encoding xylose isomerase was cloned from Thermus thermophilus and overexpressed in E. coli BL21. Purified recombinant enzyme was used to study the enzymatic characterization. Specific activity of recombinant PDOR was 19.6 U/mg. Optimal temperature and pH were 80 °C, 8.0, respectively. Km and Vmax values were 15.9 mM, 22.8 U/mg. This research may form a basis for the future application of xylose isomerase.
Preparation and Properties Analysis of Slow-Release Microcapsules Containing Patchouli Oil
Authors: Han Guang Tao , Yang Zi Ming , Peng Zheng , Wang Guang , Zhou Min , Pang Yu Xin , Li Pu Wang
Published: March 2013

Abstract: The microcapsules containing patchouli oil were prepared using a complex coacervation with chitosan and arabic gum as wall material, patchouli oil as the core material. The different factors influence on the microcapsule properties were investigated by scanning electron microscope, laser particle size analyzer, infrared spectrum and UV spectra. The best conditions for preparing patchouli oil microcapsules were confirmed as follows: the concentration of chitosan with low viscosity 0.5%, arabic gum 4%, and the ratio of wall material to core material was 2: 1. The pH value of the complex coacervation reaction was 4.5, and stirring speed was 800 r/min. The microcapsules were analyzed by Infrared spectral to confirm the patchouli oil had been successfully embedded in the microcapsules. The drug loading and encapsulation efficiency for patchouli was 20.7% and 67.2%, respectively.
Qualitative Analysis of a Ratio-Dependent Chemostat Model with Holling-(n+1) Type Functional Response
Authors: Dong Qing Lai , Sun Ming Juan
Published: March 2013

In this paper, the ratio-dependent chemostat model with Holling-(n+1) type functional response is considered. The model develops the Monod model and the ratio-dependent model. By use of the Poincar -Bendixson theory we prove the existence of limit cycle. Detailed qualitative analysis about the global asymptotic stability of its equilibria is carried out by using the Lyapunov-LaSalle invariant principle and the method of Dulac criterion.
Study on the α-Glucosidase Inhibitory Activity of Limonium bicolor Kuntze
Authors: Teng Jie Hui , Ma Li , Li Wei Lin , Yao Shun
Published: March 2013

The antidiabetic activities of the extracts with different solvents and methods from Limonium bicolor Kuntze were investigated. It was found that all the extracts with ethanol, petroleum ether, EtOAc, n-BuOH and H2O from the underground part of the plant possessed the α-Glucosidase inhibitory activity, and the fractions from the 80% ethanol extracts possessed strong activity. The extracts eluted with water from the 80% ethanol crude extract of the aerial part of the plant were inactive to α-Glucosidase inhibition, the other extracts from the aerial part all give potent α-Glucosidase inhibition activity.
Preparation of Hydroxypropyl Cyclodextrin Inclusion of Oxyresveratrol/Pterostilbene
Authors: Yu Yi Feng , Feng Wei , Zhang Yue , Wang Chun Fang , Zhao Shu Chun
Published: March 2013

Preparations, Characterizations and Release Performances of Oxyresveratrol-Hydroxypropyl Cyclodextrin Inclusion and Pterostilbene-Hydroxypropyl Cyclodextrin Inclusion Are Reported.
Microwave Pre-Treatment of Corn Straw and its Enzymatic Hydrolysis
Authors: Han Mu Xin , Li Dong Mei , Feng Yu Jie , Tan Yu Fei , Yuan Yi Xing
Published: March 2013

To enhance enzymatic hydrolysis efficiency of the corn straw, a process microwave pretreatment of corn straw was optimized using response surface methodology (RSM). Estimated optimum parameters were as follows: water to corn straw ratio is 58:1, microwave power is 640w and processing time is 5 minutes. Under these conditions, a maximum enzyme hydrolysis ratio of corn straw (49.25%) after microwave pretreatment was reached.
Purification of Total Flavonoids from Buddleja officinalis by AB-8 Macroporous Adsorption Resin
Authors: Wen Ying , Liu Ya Ting , Zhang Jing Min , Guo Lei
Published: March 2013

The separation and purification of total flavonoids from Buddleja officinalis were carried out by AB-8 macroporous adsorption resin. The optimum adsorption conditions for sample flow rate, pH value and feeding concentration were 2.0 mL/min, 4.5 and 1.6 mg/mL, respectively, and the optimum desorption conditions were obtained by using 2.0 BV of 70% ethanol as desorption solvent at a flow rate of 1.0 mL/min. Under these conditions, the flavonoids content of the final product was 90.43%, increased by 1.7 times than unpurified sample (53.09%).
The Thermodynamic Analysis of Ni1/2Mn1/2(OH)2 Prepared by Hydroxide Co-Precipitation Method
Authors: Gu Yi Jie , Zhang Qing Gang , Chen Yun Bo , Liu Hong Quan , Wang Yan Min , Hao Fei Xiang , Liu Peng , Li Shu Qi
Published: March 2013

The thermodynamic analysis of Ni2+-Mn2+-NH3-OH--H2O in co-precipitation system was carried out, and the precursor Ni1/2Mn1/2(OH)2 was prepared by hydroxide co-precipitation method. The analysis showed that the best pH value of mixed solution is 11 and the ammonia concentration is 0.4 mol/L, when NaOH is precipitating agent and ammonia is chelating agent. When the pH value is 11, the SEM images confirm that the morphology of Ni1/2Mn1/2(OH)2 is the best.
Effect of Polysaccharides from Fresh Persimmon (Diospyros kaki) Fruits and its Sulfated Derivates on the Immunomodulatory Activity of Mouse Peritoneal Macrophage Cells
Authors: Ding Yan , Zhang Ya Li , Guo Hui
Published: March 2013

The effects of polysaccharides extracted from persimmon (Diospyros kaki) fruits (PFP) and its sulfated derivates (PFP-S) on activity of mouse peritoneal macrophages (PM) were evaluated by measuring their effects on the viability, nitric oxide (NO) production, cytokine (TNF-α, IL-10 and IL-2) production and gene expression. The results showed that both PFP and PFP-S significantly increased the viability and the productions of NO, TNF-α and IL-10 with the increase of the substitution degree (DS) and dose. Of the fractions, PFP-SIII (DS 2.5, Mw 48kDa) was the most potent inducer of NO and cytokine production, increasing production of NO and expression of cytokines (IL-10 and TNF-α). It was supposed from this result that PFP-S might act as an inducer of macrophage functions against pathogens, which might be affected by the sulfate content of polysaccharides made great role in regulate of immunomodulatory activities.
Nanostructured Morphology of Adsorption of Polyelectrolyte at the Solid/Liquid Interface Observed by Atomic Force Microscopy
Authors: Xia Hong Ying , Zhao Feng
Published: March 2013

In this paper, the morphology change of various polymers at the mica –water interface, such as polyvinylpyrrolidone (PVP), polystryrenesulfonate (PSS), and partially hydrated polyacrylamide (HPAM) in different environments was investigated by atomic force microscopy (AFM). We found that PVP molecules deposited on mica from aqueous solution appeared in stretched wormlike coils forming densely packed films. In NaCl solution, the globular molecular conformation was observed. For PSS molecules the similar results were obtained. HPAM molecules deposited on the mica surface from aqueous solution at natural pH were observed to form a nanostructured network
The Application of Modified Anatase Nano-TiO2
Authors: Wu Jian Chun
Published: March 2013

The anatase nano-TiO2 was modified by metal ion-doping to improve its photocatalystic activity. Then the modified nano-TiO2 was used in coating as a formaldehyde degradation material and antibacterial materials. The test results showed that modified nano-TiO2 photocatalytic properties was significantly improved whose formaldehyde degradation rate reached 85% and bacteriostatic rate was more than 99%. Then the photocatalytic theory and the antibacterial theory of modified nano - TiO2 were analyzed.
An Amperometric Glucose Biosensor Based on Pd-Pt Nanodendrites and Chitosan Matrix
Authors: Sun Xiao Bin , Ma Zhan Fang
Published: March 2013

We report on the utilization of Pd-Pt nanodendrites (Pd-Pt NDs) to create a highly responsive glucose biosensor. Glucose oxidase was simply mixed with Pd-Pt NDs and cross-linked on the Pt electrode with chitosan (CHIT) medium by glutaraldehyde. The biosensor showed low linear detection limit (0.6 μM), wide linear detection range (0.001-5 mM). The excellent performance of the biosensor is attributed to relatively large surface areas and particularly catalytic active of Pd-Pt NDs, and good biocompatibility of CHIT, which enhances the enzyme absorption and promotes electron transfer between redox enzymes and the surface of electrodes. This composite film can be easily extended to immobilize other biomolecules.
Degradation of Congo Red in Wastewater Using Nanometer TiO2 Film under UV Light Irradiation
Authors: Tian Peng , Du Wen Le , Zheng Zhi Guo , Lu Jie , Chen Qing Yang , Sun Lu
Published: March 2013

Synthesis of titanium dioxide sol was prepared by sol-gel method, using tetrabutyl titanate as source, ethanol as solvent, acetic acid as complexing agent. A transparent sol aging in air 30min, titanium dioxide films were prepared by using spin coating method. Congo red degradation was investigated by TiO2photocatalysis. Study the efficiency of decolorization was influenced by the coating times, heat-treated time, the initial concentration of congo red and solution pH.
Large Scale Synthesis of Dendritic CdS Nanostrucutres
Authors: Sun Meng Le , Yang Yan Xin
Published: March 2013

Single crystalline CdS dendrites were successfully synthesized in high yield by a simple and facile hydrothermal method. The allyl thiourea and CdCl2•5H2O were used as raw materials for the synthesis of dendritic CdS nanostructures for the first time. The as-prepared products were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscope and selected area electron diffraction. The results demonstrate that the petal in an individual dendritic CdS nanoarchitecture is single crystalline and prefers growth along the [101] direction. The reaction parameters affected the CdS morphology were investigated systematically. It is found that the morphology of the samples are strongly dependent on the cadmium source, sulfide source, the reaction time and the solvent, the temperature has no effect on the morphology of the products. The possible mechanism was proposed for the formation of dendritic CdS nanostructures
Fabrication of Silver Nanoparticle Decorated AFM Tips for Tip-Enhanced Raman Scattering Applications
Authors: Li Yu Hua , Xu Chang Hui , Han Min
Published: March 2013

We report on a simple method for the fabrication of metalized tips by depositing gas-phase synthesized silver nanoparticles onto atomic force microscope (AFM) tips. The method enables fabricating tips suitable for AFM based tip-enhanced Raman spectroscopy (TERS) with high yield. The performance of the fabricated silver nanoparticle decorated tips is examined by detecting low concentration BPE molecules using a transmission mode TERS setup. An 30-fold enhancement of the Raman signal is shown. The net TERS enhancement factor is calculated to be about 1000.
Preparation of Squid Gelatin-Polysaccharide Scaffolds
Authors: Zhang Fei , Wang Yong Gang , Zhang Wei Jie , Wang Peng
Published: March 2013

The squid gelati were extracted by enzymatic degradation method. and Chuanxiong polysaccharide were prepared by water extract-alcohol method. 0.1% chuanxiong polysaccharide solution and 2% squid skin collagen solution were mixed, and cross-linked. A novel scaffold, the squid gelatin-polysaccharide scaffold (SGPS), was fabricated from the squid gelatin and Chuanxiong polysaccharide using glutaraldehyde as a crosslinking agent by lyophilizing.
Synthesis of Spindle-Like Hollow Mesoporous Silicas with Tunable Wall Thickness
Authors: Li Yan Xiao , Ma Zhan Fang
Published: March 2013

Spindle-like hollow mesoporous silica nanoparticles (spindle-like HMSNs) were fabricated via the template-sacrificial technique using hematite (Fe2O3) spindles as hard template, tetraethylorthosilicate (TEOS) as precursor and cetyltrimethyl ammonium bromide (CTAB) as the pore directing agent. The Fe2O3core and CTAB were selectively removed by hydrochoric acid aqueous solution etching. Transmission electron microscopy results revealed that spindle-like HMSNs with thickness of shell range from 8 to 47 nm were prepared by changing the concentration of the reactant. This methodology provides a facile and robust route for preparation of hollow mesoporous materials.
Low Temperature CO Oxidation over Cobalt Catalysts Supported on Mesoporous CeO2
Authors: Tian Jun Jie , Na Wei , Wang Hua , Gao Wen Gui
Published: March 2013

Mesoporous CeO2 was first synthesized by hydrothermal method, and then used to synthesize different content of (Co3O4)x/CeO2 (x was the molar ratio of Cu and Co) by deposition-precipitation method. The fresh and doped catalysts were characterized by X-ray diffraction (XRD), N2 adsorption and desorption, H2 temperature programmed reduction (H2-TPR) and O2 temperature programmed desorption (O2-TPD) to study the crystal structure, surface area, and the mechanism of CO oxidation. The results show that: In XRD pattems, the doped cobalt amounts of samples from x=20% to x=100% have Co3O4 crystal structure. The N2 adsorption and desorption indicated the samples were mesoporous structure. Compared with other samples, the better reducibility and activity oxygen species of (Co3O4)50%/CeO2 coincided with its better catalytic activity.
Thermoresponsive Ion-Imprinted Hydrogels with Interpenetrating Network Structure for Removal of Heavy Metal Ions
Authors: Wang Jing Jing , Liu Fang
Published: March 2013

A novel thermoresponsive Cu(II) ion-imprinted hydrogel [Cu(II)-IIH] with interpenetrating network structure has been prepared to remove Cu(II) ions with high selectivity. The Cu(II)-IIH was prepared by free radical/cationic hybrid photopolymerization of N-isopropylacrylamide (NIPAM) and triethylene glycol divinyl ether (DVE-3) using Cu(II) ion as template. The Cu(II)-IIH was used to remove heavy metal ions from aqueous solution at 20 and 40oC. The Cu(II)-IIH selectively bound the template ion above a lower critical solution temperature (LCST) of poly(N-isopropylacrylamide) (PNIPAM). The memory was fixed by shrinking above the LCST, and was deleted by swelling below the LCST. Selective adsorption study indicated that ion imprinting resulted in stronger affinity of the Cu(II)-IIH toward Cu(II) ions than other competitor metal ions compared with the non-imprinted hydrogel (NIH).
Feedback Linearization Control for PMSG Wind Power Generation System with Application of Wind Turbine in Mechanical Engineering
Authors: Li Na , Wang Wu
Published: March 2013

The fluctuation, intermittence and uncertain are the characteristics of wind energy which cause wind energy output power fluctuation, so the wind power generation system was a typical nonlinear system, which was hard to be controlled exactly by traditional controllers, so, the feedback linearization control was applied to wind power generation system (WPGS) in mechanical engineering, which was to reduce the cost of wind energy conversion system and improve its performance. Feedback linearization control contains coordinate transformation, Lie derivative solving, and inverse coordinate transformation module, the control strategy was proposed. The WPGS model was constructed under MATLAB platform, with feedback linearization control theory based on differential geometry, the coordinated transformation and nonlinear state feedback were obtained. the simulation parameters was designed and the simulation result shows the control model was stable and direct feedback linearization control with higher tracking performance, which can effectively implement maximum energy capture.
Control Simulation for Two-Wheeled Self-Balancing Robot Linear Move Based on Active Disturbance Rejection Controller
Authors: Ye Da Peng , Yu Jia Ming , Zhou Yi Zhang
Published: March 2013

To achieve a sound control for the body displacement and angle of two-wheeled self-balancing robot system, which is strong coupling, nonlinear, parameter uncertainty and hard to control, through the design of two Active Disturbance Rejection Controllers (ADRCs) for their body angle and displacement, respectively. The method compares with classical pole placement feedback control methods in the case of the robot model parameter perturbation for simulation. The results show that the ADRC method has a more robust than the pole placement one for the control object parameter.
Influence of Microstructures on Wettability on Stainless Steel
Authors: Fu Ting , Tang Yong , Guo Liang , Wan Zhen Ping , Luo Wen Jie
Published: March 2013

The influence of microstructures on wettability on stainless steel was investigated. Two kinds of different microstructures were manufactured by a laser processing method with micro parallel grating and square pillars array. The experimental and theoretical values of contact angle (CA) were obtained. Furthermore, within the groove spacing processing size range in micron scale, the experimental results agreed with the theoretical predictions based on the Wenzel. By the influence of machining and measuring conditions, the experimental values of CA were smaller than the predicted theoretical values. A linear relationship between the CA and the micro-scale structures was obtained, which showed the CA was increased with an increase of groove spacing. With the same structural parameters, the CA of square pillars array microstructures was larger than parallel grating microstructures.
Traffic Sign Recognition Method in Intelligent Transport System Based on the Low-Rank Approximation
Authors: Xiao Ming Xi , Zhang Han Ling
Published: March 2013

This paper presents a new method for traffic sign recognition in Intelligent Transport System,which base on low-rank approximation and support vector machine (SVM),the method including traffic signs correction and SVM identification.first we extraction traffic sign region and internal texture,according to the characteristics of internal texture,combine with the spare and low-rank approximation,to correct the texture automatically ,next to extract the feature vectors of traffic signs texture,finally identification in the database.The experimental results show: the method base on low-rank approximation can corrected the deformation traffic signs effectively and accurately,improve the recognition rate of the SVM,it has good feasibility and real-time.
Effect of Notch Depth on the Butt-Joint under Izod Impact Test with Material Properties and Mechanics Analysis
Authors: Zheng Xiao Ling , Li Mei , You Min , Liu Wen Jun , Liu Kai
Published: March 2013

The effect of the notch depth on the impact toughness of the adhesively bonded steel butt joint under Izod impact test is studied using both the finite element method and experimental method. The results obtained from numerical simulation showed that the response time with the peak stress Seqv occurred becomes little longer when the notch depth increased from 2 mm to 8 mm. And a negative longitudinal stress occurred when there is an 8 mm depth notch which might be beneficial to subject impact load. The results from the experiments showed that the effect of notch depth is evidently on the Izod impact properties. The impact energy absorbed by unit area of joint is increased as the notch depth increase
A New Design of Pipe Robot with Mechanical Properties in Mechanical Engineering
Authors: Nian Si Cheng , Sun Han Xu , Zhang Yan Heng , Chen Wei
Published: March 2013

A new squirming robot is designed to clean up crystal substance in chemical pipelines. The robot uses a ball screw and a motor as its driven unit, Its mechanical properties is efficiently in the pipeline. One of its advantages is its strong driven force, which is proved by theoretical analysis. The robot can clean the pipeline by some tools at the front. Through theoretical analysis, the mechanical structure can solve the pipeline environmental effects on mechanical properties
Theoretical Analysis and Experimental Research on Cluster Bomb Warhead Built Pressure Device Based on Mechanical Mechanics
Authors: Zhang Han
Published: March 2013

This paper according to a certain type of cluster bomb warhead built pressure chamber technical index requirements, design process a set of gas pressure device built principle prototype, and experimental study was carried out. Based on gas build pressure device working process research has determined its three working section: pressure rise section, pressure balance segment and pressure drop section, and then according to the rocket engine interior ballistic principle for each work period respectively calculated, get gas build pressure device and build pressure chamber of the interior ballistic pressure curve, and finally use of principle prototype experimental study. The theoretical calculation curve and test the measured curve have very good consistency; prove this paper established pressure calculation model is reasonable and credible for future related problems research provides a basis.
The Research on End-Point Forecast and End-Point Control of Argon Oxygen Refining Ferroalloy with Material Properties
Authors: Ke Hong Di , Wang Hui
Published: March 2013

In this article, we studied Ferroalloy Smelting process, we made use of AOD furnace to smell Ferroalloy. For AOD furnace high temperature, and more dust, AOD furnace mechanism model nonlinear, and uncertainty, and strong coupled features, Aiming at these features we designed argon oxygen refining ferroally’s end_point forecast and end_point control tactics which based on online detection. Then we constructed end_point forecast model and end_point control model according to the tactics. We selected Jilin Ferroalloy 5 tons AOD furnace 50 sets of measured data,then trained and assessed the established forecast model with measured data, through MATLAB simulation obtained better of forecast results.
Noise Control on Chiller with Ammonia Based on Vibration-Acoustics Analysis in Mechanical Engineering
Authors: Liu Huan , Chen Chang Zheng , Zhou Hao
Published: March 2013

Noise detection and vibration analysis have been made in the enterprise where screw compressor unit has been running with problems for a long term. According to the characteristics of noise and vibration spectrum, ammonia refrigeration operation fault and noise source have been analyzed, and a noise reduction scheme is made. Based on this, through the optimization of compound sound absorbing structure parameters design and manufacturing device of sound insulation, thereby hidden safety trouble can be eliminated and the working environment of workers can be improved. Besides, mechanical properties of the compressor group are more stable. In practical engineering applications, good effect of noise reduction has been achieved.
Study on Calculations of Geometric Dimensions and Modification Coefficients of Planetary Gear Reducer of Less Tooth Difference with Biasing Crankshaft Based on Matlab
Authors: Xu Qiang , Xu Qi Sheng , Xu Dao Yi
Published: March 2013

Different formula characteristics of internal gear pair with few teeth difference of planetary gear reducer with biasing crankshaft were compared, and the restricted conditions satisfied by them when meshing were set forth. On this basis, the relationships of modification coefficients, tooth addendum coefficient and the center distance were studied, the optimal choice method for modification coefficient based on matlab for the smallest meshing angle was proposed, and verified with living example, at the same time the effect of the different step lengths on calculation results was researched. The results showed that the optimum method is accurate, and avoids the disadvantages of the other optimal choice methods.
Diagnosis Research on Main Bearing Wear of Gasoline Engines in Mechanical Engineering
Authors: Wang Chang Shun
Published: March 2013

The different clearances of main bearing of previously designed on EQ6100 model gasoline engine is diagnosed by means of vibration monitoring mechanism. Breakdown signals of main test on different speed, clearance of main bearing, test spot and weather were analyzed by Spectral Analysis method and compared with normal and abnormal vibration signals. As a result, the characteristic parameters and the identifying methods of breakdown are given. In addition, the problems of fault detection are pointed out.
Research on Analysis of Lubrication Oil in Fault Diagnosis of Vehicle Engine in Mechanical Engineering
Authors: Gu Jun , Zhou Jing Jing
Published: March 2013

The malfunction of Engine is expounded systematically due to engine lubricating oil. Common malfunction mode and Studies, oil technology analysis and abrasive particle spectrum make method of diagnosis and process possible. Contrast of oil abrasive particle with spectrum helps to find out the malfunction cause and wear part.
Wind Turbine Gearbox Fault Detection Based on Multifractal Analysis
Authors: Chen Chang Zheng , Pan Ping Ping , Meng Qiang , Gu Yan Ling
Published: March 2013

The presence of irregularity in periodical vibration signals usually indicates the occurrence of wind turbine gearbox faults. Unfortunately, detecting the incipient faults is a difficult job because they are rather weak and often interfered by heavy noise and higher level macro-structural vibrations. Therefore, a proper signal processing method is necessary. We used the wavelet-based multifractal method to extract the impulsive features buried in noisy vibration signals. We first calculated the wavelet transform modulo maxima lines from the real vibration signals, then, obtained the singularity spectrum from the lines. The analysis results of the real signals showed that the proposed method can effectively extract weak fault features.
Research on Geothermal Energy Using RS Land Surface Material with Temperature Inversion Technology
Authors: Li Jian Feng , Chen Jian Ping
Published: March 2013

Taking Tengchong area in Yunnan Province as a study area and ASTER and ETM remote sensing images as research object, authors selected the proper method for the study area by Comparing the results of the Land Surface Material Temperature Inversion. The result shows that the ETM inversion method is more precise than the ASTER method. Both methods can meet the demand of temperature classification.
Three Dimensional Finite Element Analysis of Reinforced Concrete Rectangular-Sectioned Aqueduct
Authors: Ji Dong Yu
Published: March 2013

This paper adopts general finite element software to carry out three-dimensional finite element simulation analysis for Huizeli reinforced concrete rectangular-sectioned aqueduct. Considering four combination cases in aqueduct’s construction and operating process, researching variation laws of the aqueduct’s stress and displacement. Analysis results show that design scheme of Huizeli reinforced concrete rectangular-sectioned aqueduct is reasonable, it can meet design requirements. Analysis results provide some theory references for design of reinforced concrete rectangular-sectioned aqueduct.
Research on the Remote Monitoring System of Photovoltaic Power Station Based on CAN Bus and GPRS with Mechanical Properties
Authors: Li Kun Fu , Luo Wei Ping
Published: March 2013

According to the requirements of the PV station remote monitoring system, such as big information flow, far distance transmission, high-performance, this article has designed a remote PV station monitoring system based on CAN bus and GPRS with mechanical properties. After lucubrated CAN bus and GPRS communication technology, this article details the hardware circuit of the slave computer, the communication protocol of CAN bus and the design of communication network. It has solved the problems that bad real-time, low degree of automation, few nodes can be accommodated, weak anti-interference ability which exits in the past design of PV station remote monitoring system.