bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of BBB Dysregulation in Neuropathogenicity Using Integrative Human Model of Blood–Brain Barrier
Authors: Simöes Da Gama Coraly , Morin-Brureau Mélanie
Published: June 2022

The blood–brain barrier (BBB) is a cellular and physical barrier with a crucial role in homeostasis of the brain extracellular environment. It controls the imports of nutrients to the brain and exports toxins and pathogens. Dysregulation of the blood–brain barrier increases permeability and contributes to pathologies, including Alzheimer's disease, epilepsy, and ischemia. It remains unclear how a dysregulated BBB contributes to these different syndromes. Initial studies on the role of the BBB in neurological disorders and also techniques to permit the entry of therapeutic molecules were made in animals. This review examines progress in the use of human models of the BBB, more relevant to human neurological disorders. In recent years, the functionality and complexity ofin vitroBBB models have increased. Initial efforts consisted of static transwell cultures of brain endothelial cells. Human cell models based on microfluidics or organoids derived from human-derived induced pluripotent stem cells have become more realistic and perform better. We consider the architecture of different model generations as well as the cell types used in their fabrication. Finally, we discuss optimal models to study neurodegenerative diseases, brain glioma, epilepsies, transmigration of peripheral immune cells, and brain entry of neurotrophic viruses and metastatic cancer cells.
Immune Cells in Subretinal Wound Healing and Fibrosis
Authors: Szczepan Manon , Llorián-Salvador María , Chen Mei , Xu Heping
Published: June 2022

The subretinal space is devoid of any immune cells under normal conditions and is an immune privileged site. When photoreceptors and/or retinal pigment epithelial cells suffer from an injury, a wound healing process will be initiated. Retinal microglia and the complement system, as the first line of retinal defense, are activated to participate in the wound healing process. If the injury is severe or persists for a prolonged period, they may fail to heal the damage and circulating immune cells will be summoned leading to chronic inflammation and abnormal wound healing, i.e., subretinal or intraretinal fibrosis, a sight-threatening condition frequently observed in rhematogenous retinal detachment, age-related macular degeneration and recurrent uveoretinitis. Here, we discussed the principles of subretinal wound healing with a strong focus on the conditions whereby the damage is beyond the healing capacity of the retinal defense system and highlighted the roles of circulating immune cells in subretinal wound healing and fibrosis.
Investigating Acupoint Selection and Combinations of Acupuncture for Tic Disorders: An Association Rule Mining and Network Analysis Study
Authors: Chen Jieting , Xie Yufeng , Lin Qingchan , Qian Ziliang , Feng Jun , Zhang Jianmei , Chen Yun , Chen Wenhan , Wu Yueting , Guo Ziyi
Published: June 2022

Objective

Tic disorders (TDs) are common mental disorders in children and adolescents, and the clinical application of acupuncture for treating TDs is becoming increasingly widespread. However, the criteria for selecting acupoint prescriptions and combinations have not been summarized. Therefore, data mining was used herein to determine the treatment principles and the most effective acupoint selection and compatibility criteria for the treatment of TDs.

Methods

Clinical studies and observations of the efficacy of acupuncture treatment for TDs were obtained from the PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure (CNKI), Wanfang, VIP, and Chinese Biomedical (CBM) databases. The data on the acupoint prescriptions applied in these studies were collected, and network and association analyses were used to reveal the relationships between acupoints and to identify acupoint combinations. Additionally, the principles of acupuncture for TDs were determined through cluster analysis. Subgroup analysis of acupuncture prescriptions based on specific categorical diagnoses was performed to further assess the selection of acupoints.

Results

Eighty-six trials were identified, and 257 groups of effective prescriptions involving 121 acupoints were extracted. Bai-hui (DU20), Feng-chi (GB20), Tai-chong (LR3), He-gu (LI4), and San-yin-jiao (SP6) were the most regularly used acupoints for treating TDs. The Governor Vessel, gallbladder, and large intestine meridians were more commonly used than other meridians. Moreover, most acupoint sites focused on the head and neck. Network analysis revealed potentially effective acupoint prescriptions for their commonly used acupoints, namely, Bai-hui (DU20), Si-shen-cong (EX-HN1), Feng-chi (GB20), Nei-guan (PC6), Shen-men (HT7), He-gu (LI4), Zu-san-li (ST36), San-yin-jiao (SP6) and Tai-chong (LR3). Association rule mining indicated that potential point combinations that should be prioritized in TD treatment are Bai-hui (DU20), Neiguan (PC6) and Sanyinjiao (SP6). Cluster analysis revealed the treatment principle of “coordinating yin and yang, tonifying qi and blood, dispelling pathogenic wind and eliminating phlegm”. The core acupoint prescription of TS treatment comprised He-gu (LI4), Feng-chi (GB20), Tai-chong (LR3), Bai-hui (DU20), Yin-tang (EX-HN3), Si-shen-cong (EX-HN1), San-yin-jiao (SP6), and Nei-guan (PC6). The core group included He-gu (LI4) and Feng-chi (GB20). Proximal points were usually used in TS as an additional method of point selection.

Conclusion

Using data mining analysis of published studies, this study provides valuable information regarding the selection of the most effective acupoints and point combinations for clinical acupuncture practice for treating TDs.
Building Human Visual Attention Map for Construction Equipment Teleoperation
Authors: Fan Jiamin , Li Xiaomeng , Su Xing
Published: June 2022

Construction equipment teleoperation is a promising solution when the site environment is hazardous to operators. However, limited situational awareness of the operator exists as one of the major bottlenecks for its implementation. Virtual annotations (VAs) can use symbols to convey information about operating clues, thus improving an operator’s situational awareness without introducing an overwhelming cognitive load. It is of primary importance to understand how an operator’s visual system responds to different VAs from a human-centered perspective. This study investigates the effect of VA on teleoperation performance in excavating tasks. A visual attention map is generated to describe how an operator’s attention is allocated when VAs are presented during operation. The result of this study can improve the understanding of how human vision works in virtual or augmented reality. It also informs the strategies on the practical implication of designing a user-friendly teleoperation system.
Resting Rates of Blood Flow and Glucose Use per Neuron Are Proportional to Number of Endothelial Cells Available per Neuron Across Sites in the Rat Brain
Authors: Ventura-Antunes Lissa , Dasgupta Oisharya Moon , Herculano-Houzel Suzana
Published: June 2022

We report in a companion paper that in the mouse brain, in contrast to the 1,000-fold variation in local neuronal densities across sites, capillary density (measured both as capillary volume fraction and as density of endothelial cells) show very little variation, of the order of only fourfold. Here we confirm that finding in the rat brain and, using published rates of local blood flow and glucose use at rest, proceed to show that what small variation exists in capillary density across sites in the rat brain is strongly and linearly correlated to variations in local rates of brain metabolism at rest. Crucially, we show that such variations in local capillary density and brain metabolism are not correlated with local variations in neuronal density, which contradicts expectations that use-dependent self-organization would cause brain sites with more neurons to have higher capillary densities due to higher energetic demands. In fact, we show that the ratio of endothelial cells per neuron serves as a linear indicator of average blood flow and glucose use per neuron at rest, and both increase as neuronal density decreases across sites. In other words, because of the relatively tiny variation in capillary densities compared to the large variation in neuronal densities, the anatomical infrastructure of the brain is such that those sites with fewer neurons have more energy supplied per neuron, which matches a higher average rate of energy use per neuron, compared to sites with more neurons. Taken together, our data support the interpretation that resting brain metabolism is not demand-based, but rather limited by its capillary supply, and raise multiple implications for the differential vulnerability of diverse brain areas to disease and aging.
Inflammatory Biomarkers for Outcome Prediction in Patients With Metastatic Testicular Cancer
Authors: Bleve Sara , Cursano Maria Concetta , Casadei Chiara , Schepisi Giuseppe , Menna Cecilia , Urbini Milena , Gianni Caterina , De Padova Silvia , Filograna Alessia , Gallà Valentina , Rosti Giovanni , Barone Domenico , Chovanec Michal , Mego Michal , De Giorgi Ugo
Published: June 2022

Germ cell tumors are the most common malignant tumors in male young adults. Platinum-based chemotherapy has dramatically improved the outcome of metastatic germ cell tumor patients and overall cure rates now exceed 80%. The choice of medical treatment can be guided by the prognosis estimation which is an important step during the decision-making process. IGCCCG classification plays a pivotal role in the management of advanced disease. However, histological and clinical parameters are the available factors that condition the prognosis, but they do not reflect the tumor’s molecular and pathological features and do not predict who will respond to chemotherapy. After first-line chemotherapy 20%-30% of patients relapse and for these patients, the issue of prognostic factors is far more complex. Validated biomarkers and a molecular selection of patients that reflect the pathogenesis are highly needed. The association between cancer-related systemic inflammation, tumorigenesis, and cancer progression has been demonstrated. In the last years, several studies have shown the prognostic utility of immune-inflammation indexes in different tumor types. This review analyzed the prognostic impact of inflammatory markers retrieved from routine blood draws in GCT patients.
An Observational Study of Therapeutic Hypothermia and Factors Associated With Mortality in Late-Preterm and Term Neonates With Hypoxic-Ischemic Encephalopathy in a Middle-Income Country
Authors: Boo Nem Yun , Neoh Siew Hong , Chee Seok Chiong ,   for the Malaysian National Neonatal Registry
Published: June 2022

Objectives

To investigate the types of therapeutic hypothermia (TH) used and risk factors associated with mortality in late-preterm and term neonates (LPTN, gestation of ≥35 weeks) with hypoxic-ischemic encephalopathy (HIE) in a middle-income country.

Design

This was an observational retrospective cohort study.

Setting

A total of 44 neonatal intensive care units (NICUs) in the Malaysian National Neonatal Registry participated in the study.

Patients

All LPTN without major malformations and diagnosed to have HIE were included.

Main Outcome Measures

Number of in-hospital mortality, and types of TH used [no TH, TH using commercially available servo-controlled devices (SCDs), passive TH by exposing neonates to NICU’s air-conditioned ambient temperature with/without the use of cooled gel packs (P±CGPs)].

Results

Of a total of 2,761 HIE neonates, 66.3% received TH. All NICUs provided TH; 55.4% NICUs had SCDs, which was administered to 43.6% (248/569) of severe, 51.6% (636/1,232) of moderate, and 18.6% (179/960) of mild HIE neonates. P±CGPs was used on 26.9% of severe, 33.4% of moderate, and 21.1% of mild HIE neonates. There were 338 deaths. Multiple logistic regression analysis showed that 5-min Apgar scores <5 (aOR: 1.436; 95% CI: 1.019, 2.023), Cesarean section (aOR: 2.335; 95% CI: 1.700, 3.207), receiving no TH (aOR: 4.749; 95% CI: 3.201, 7.045), TH using P±CGPs (aOR: 1.553; 95% CI: 1.031, 2.338), NICUs admitted <50 HIE cases (aOR: 1.898; 95% CI: 1.225, 2.940), NICUs admitted 50-<100 HIE cases (aOR: 1.552; 95% CI: 1.065, 2.260), moderate HIE (aOR: 2.823; 95% CI: 1.495, 5.333), severe HIE (aOR: 34.925, 95% CI: 18.478, 66.012), Thompson scores of 7–13 (aOR: 1.776; 95% CI: 1.023,3.082), Thompson scores of ≥14 (aOR: 3.641; 95% CI: 2.000, 6.629), pneumothorax (aOR: 3.435; 95% CI: 1.996, 5.914), and foreigners (aOR: 1.646; 95% CI: 1.006, 2.692) were significant risk factors associated with mortality.

Conclusion

Both SCD and P±CGP were used for TH. Moderate/severe HIE and receiving passive/no TH were among the risk factors associated with mortality.
Phylogenomics Reveals the Evolutionary History of Phytolacca (Phytolaccaceae)
Authors: Song Yun , Jiang Fan , Shi Junxia , Wang Chaonan , Xiang Ning , Zhu Shuifang
Published: June 2022

Phytolacca is the largest genus of Phytolaccaceae. Owing to interspecific hybridization, infraspecific variation, and apparent weak genetic control of many qualitative characters, which have obscured boundaries between species, the classification and phylogenetic relationships of this genus are unclear. Native Phytolacca is disjunctly distributed in America, eastern Asia, and Africa, and the biogeographic history of the genus remained unresolved. In this study, we used the whole chloroplast genome and three markers (nrDNA, rbcL, and matK) to reconstruct phylogenetic relationships within Phytolacca, analyze divergence times, and infer biogeographic histories. The phylogenetic results indicate that Phytolacca is monophyletic, which is inconsistent with the infrageneric classification based on morphology. According to the divergence time estimation, Phytolacca began to diversify at approximately 20.30 Ma during the early Miocene. Central America, including Mexico, Costa Rica, and Colombia, is the center of species diversity. Biogeographical analysis indicated five main dispersal events and Phytolacca originated from Central and South America. Birds may be the primary agents of dispersal because of the fleshy fruiting of Phytolacca. This study extended sampling and added more genetic characteristics to infer the evolutionary history of Phytolacca, providing new insights for resolving the classification and elucidating the dispersal events of Phytolacca.
Integrated Transcriptomic and Proteomic Analyses Uncover the Regulatory Mechanisms of Myricaria laxiflora Under Flooding Stress
Authors: Li Linbao , Huang Guiyun , Xiang Weibo , Zhu Haofei , Zhang Haibo , Zhang Jun , Ding Zehong , Liu Jihong , Wu Di
Published: June 2022

Flooding is one of the major environmental stresses that severely influence plant survival and development. However, the regulatory mechanisms underlying flooding stress remain largely unknown in Myricaria laxiflora, an endangered plant mainly distributed in the flood zone of the Yangtze River, China. In this work, transcriptome and proteome were performed in parallel in roots of M. laxiflora during nine time-points under the flooding and post-flooding recovery treatments. Overall, highly dynamic and stage-specific expression profiles of genes/proteins were observed during flooding and post-flooding recovery treatment. Genes related to auxin, cell wall, calcium signaling, and MAP kinase signaling were greatly down-regulated exclusively at the transcriptomic level during the early stages of flooding. Glycolysis and major CHO metabolism genes, which were regulated at the transcriptomic and/or proteomic levels with low expression correlations, mainly functioned during the late stages of flooding. Genes involved in reactive oxygen species (ROS) scavenging, mitochondrial metabolism, and development were also regulated exclusively at the transcriptomic level, but their expression levels were highly up-regulated upon post-flooding recovery. Moreover, the comprehensive expression profiles of genes/proteins related to redox, hormones, and transcriptional factors were also investigated. Finally, the regulatory networks of M. laxiflora in response to flooding and post-flooding recovery were discussed. The findings deepen our understanding of the molecular mechanisms of flooding stress and shed light on the genes and pathways for the preservation of M. laxiflora and other endangered plants in the flood zone.
Sinusoidal Vibration Source Localization in Two-Dimensional Space Around the Hand
Authors: Ujitoko Yusuke , Kuroki Scinob
Published: June 2022

There are use cases where presenting spatial information via the tactile sense is useful (e.g., situations where visual and audio senses are not available). Conventional methods that directly attach a vibrotactile array to a user's body present spatial information such as direction by having users localize the vibration source from among the sources in the array. These methods suffer from problems such as heat generation of the actuator or the installation cost of the actuators in a limited space. A promising method of coping with these problems is to place the vibrotactile array at a distance from the body, instead of directly attaching it to the body, with the aim of presenting spatial information in the same way as the conventional method. The present study investigates the method's effectiveness by means of a psychophysical experiment. Specifically, we presented users with sinusoidal vibrations from remote vibrotactile arrays in the space around the hand and asked them to localize the source of the vibration. We conducted an experiment to investigate the localization ability by using two vibration frequencies (30 Hz as a low frequency and 230 Hz as a high frequency). We chose these two frequencies since they effectively activate two distinctive vibrotactile channels: the rapidly adapting afferent channel and the Pacinian channel. The experimental results showed that humans can recognize the direction of the vibration source, but not the distance, regardless of the source frequency. The accuracy of the direction recognition varied slightly according to the vibration source direction, and also according to the vibration frequency. This suggests that the calibration of stimulus direction is required in the case of both high and low frequencies for presenting direction accurately as intended. In addition, the accuracy variance of direction recognition increased as the source became farther away, and the degree of increase was especially large with the low-frequency source. This suggests that a high frequency is recommended for presenting accurate direction with low variance.
Effects of 16 Weeks of Cheerleading on Physical Self-Esteem and Mental Health of Female College Students
Authors: Deng Chenliang , Yu Qiaoyan , Luo Ganglin , Lu Shuiping
Published: June 2022

Objective

This study aimed to analyze the influence of cheerleading on female college students’ physical self-esteem and mental health.

Materials and Methods

In total, 63 female college students from the University of Electronic Science and Technology of China were trained in cheerleading for 16 weeks. The scores of each sub-field of physical self-esteem and psychological symptoms were analyzed by using Physical Self-Perception Profile (PSPP) and Symptom Checklist 90 (SCL-90), respectively, at 0 and 16 weeks of the experiment.

Results

After 16 weeks of cheerleading exercise, female college students had significant differences in physical attractiveness (T = 4.18), physical quality (T = 4.39), and physical self-worth (T = 3.35) before and after the experiment (P < 0.01). There were significant differences in physical condition (T = 3.87) and athletic ability (T = 2.88) before and after the experiment (P < 0.05). Somatization (T = 6.485), obsessive-compulsive symptoms (T = 11.716), interpersonal sensitivity (T = 10.077), depression (T = 8.403), anxiety (T = 10.767), hostility (T = 10.866), terror (T = 9.260), paranoia (T = 10.414), psychosis (T = 9.610), sleep and eating disorders (T = 9.323), total symptom index (T = 13.245), and mean score of positive symptoms (T = 12.237) were significantly different (P < 0.01).

Conclusion

Cheerleading can significantly improve the level of female college students’ physical self-esteem, especially the self-esteem level of physical attractiveness, physical quality, and physical self-worth. They also experienced significant improvement in their psychological disorders, especially somatization, depression, and sleep and eating disorders, which effectively improved their overall mental health.
Obesity and Type 2 Diabetes Mellitus Explained by the Free Energy Principle
Authors: Peters Achim , Hartwig Mattis , Spiller Tobias
Published: June 2022

According to the free energy principle, all sentient beings strive to minimize surprise or, in other words, an information-theoretical quantity called variational free energy. Consequently, psychosocial “stress” can be redefined as a state of “heightened expected free energy,” that is, a state of “expected surprise” or “uncertainty.” Individuals experiencing stress primarily attempt to reduce uncertainty, or expected free energy, with the help of what is called an uncertainty resolution program (URP). The URP consists of three subroutines: First, an arousal state is induced that increases cerebral information transmission and processing to reduce uncertainty as quickly as possible. Second, these additional computations cost the brain additional energy, which it demands from the body. Third, the program controls which stress reduction measures are learned for future use and which are not. We refer to an episode as “good” stress, when the URP has successfully reduced uncertainty. Failure of the URP to adequately reduce uncertainty results in either stress habituation or prolonged toxic stress. Stress habituation reduces uncertainty by flattening/broadening individual goal beliefs so that outcomes previously considered as untenable become acceptable. Habituated individuals experience so-called “tolerable” stress. Referring to the Selfish Brain theory and the experimental evidence supporting it, we show that habituated people, who lack stress arousals and therefore have decreased average brain energy consumption, tend to develop an obese type 2 diabetes mellitus phenotype. People, for whom habituation is not the free-energy-optimal solution, do not reduce their uncertainty by changing their goal preferences, and are left with nothing but “toxic” stress. Toxic stress leads to recurrent or persistent arousal states and thus increased average brain energy consumption, which in turn promotes the development of a lean type 2 diabetes mellitus phenotype. In conclusion, we anchor the psychosomatic concept of stress in the information-theoretical concept of uncertainty as defined by the free energy principle. In addition, we detail the neurobiological mechanisms underlying uncertainty reduction and illustrate how uncertainty can lead to psychosomatic illness.
Sex Differences in Bipolar Disorders: Impact on Psychopathological Features and Treatment Response
Authors: Menculini Giulia , Steardo Luca , Sciarma Tiziana , D'Angelo Martina , Lanza Laura , Cinesi Gianmarco , Cirimbilli Federica , Moretti Patrizia , Verdolini Norma , De Fazio Pasquale , Tortorella Alfonso
Published: June 2022

Introduction

Sex differences were demonstrated in bipolar disorders (BD) concerning epidemiological, clinical, and psychopathological characteristics, but consensus is lacking. Moreover, data concerning the influence of sex on treatment response in BD is contrasting. The present cross-sectional study aimed to analyze sex differences in a population of BD subjects, with specific focus on psychopathological features and treatment response.

Materials and Methods

Subjects diagnosed with BD according to the Diagnostic and Statistical Manual of Mental Disorders, 5th version (DSM-5) were recruited. Socio-demographic and clinical characteristics were collected. The Hamilton Rating Scale for Depression, the Mania Rating Scale (MRS), the brief version of the Temperament Evaluation of Memphis, Pisa and San Diego—Münster version (briefTEMPS-M), and the Barratt Impulsiveness Scale−11 items (BIS-11) were used for psychopathological assessment. Treatment response was appraised with the Alda Scale. We performed bivariate analyses to compare socio-demographic, clinical, and psychopathological characteristics between men and women (p < 0.05). A logistic regression was run to analyze features that were significantly associated with female sex.

Results

Among the recruited 219 BD subjects, 119 (54.3%) were females. Women had a lower scholarity (p = 0.015) and were less frequently employed (p = 0.001). As for psychopathological features, a higher MRS total score (p < 0.001) was detected among women, as well as higher BIS-11 total score (p = 0.040), and briefTEMPS-M score for anxious temperament (p = 0.006). Men showed higher prevalence of DSM-5 mixed features (p = 0.025), particularly during a depressive episode (p = 0.014). Women reported longer duration of untreated illness (DUI) (p < 0.001). There were no sex differences in the Alda Scale total score when considering the whole sample, but this was significantly higher among men (p = 0.030) when evaluating subjects treated with anticonvulsants. At the logistic regression, female sex was positively associated with longer DUI (p < 0.001; OR 1.106, 95% CI 1.050–1.165) and higher MRS total score (p < 0.001; OR 1.085, 95% CI 1.044–1.128) and negatively associated with employment (p = 0.003; OR 0.359, 95% CI 0.185–0.698) and DSM-5 mixed features (p = 0.006; OR 0.391, 95% CI 0.200–0.762).

Conclusions

The clinical presentation of BD may differ depending on sex. The severity of BD should not be neglected among women, who may also display worse treatment response to anticonvulsants.
Comparison of Image Quality and Radiation Dose Between Single-Energy and Dual-Energy Images for the Brain With Stereotactic Frames on Dual-Energy Cerebral CT
Authors: Zhao Xiaojing , Chao Wang , Shan Yi , Li Jingkai , Zhao Cheng , Zhang Miao , Lu Jie
Published: June 2022

Background

Preoperative stereotactic planning of deep brain stimulation (DBS) using computed tomography (CT) imaging in patients with Parkinson's disease (PD) is of clinical interest. However, frame-induced metal artifacts are common in clinical practice, which can be challenging for neurosurgeons to visualize brain structures.

Objectives

To evaluate the image quality and radiation exposure of patients with stereotactic frame brain CT acquired using a dual-source CT (DSCT) system in single- and dual-energy modes.

Materials and Methods

We included 60 consecutive patients with Parkinson's disease (PD) and randomized them into two groups. CT images of the brain were performed using DSCT (Group A, an 80/Sn150 kVp dual-energy mode; Group B, a 120 kVp single-energy mode). One set of single-energy images (120 kVp) and 10 sets of virtual monochromatic images (50–140 keV) were obtained. Subjective image analysis of overall image quality was performed using a five-point Likert scale. For objective image quality evaluation, CT values, image noise, signal-to-noise ratio (SNR), and contrast-to-noise (CNR) were calculated. The radiation dose was recorded for each patient.

Results

The mean effective radiation dose was reduced in the dual-energy mode (1.73 mSv ± 0.45 mSv) compared to the single-energy mode (3.16 mSv ± 0.64 mSv) (p < 0.001). Image noise was reduced by 46–52% for 120–140 keV VMI compared to 120 kVp images (both p < 0.01). CT values were higher at 100–140 keV than at 120 kVp images. At 120–140 keV, CT values of brain tissue showed significant differences at the level of the most severe metal artifacts (all p < 0.05). SNR was also higher in the dual-energy mode 90–140 keV compared to 120 kVp images, showing a significant difference between the two groups at 120–140 keV (all p < 0.01). The CNR was significantly better in Group A for 60–140 keV VMI compared to Group B (both p < 0.001). The highest subjective image scores were found in the 120 keV images, while 110–140 keV images had significantly higher scores than 120 kVp images (all p < 0.05).

Conclusion

DSCT images using dual-energy modes provide better objective and subjective image quality for patients with PD at lower radiation doses compared to single-energy modes and facilitate brain tissue visualization with stereotactic frame DBS procedures.
High Molecular Weight λ-Carrageenan Improves the Color Stability of Phycocyanin by Associative Interactions
Authors: Buecker Stephan , Grossmann Lutz , Loeffler Myriam , Leeb Elena , Weiss Jochen
Published: June 2022

Phycocyanin is a protein-chromophore structure present in Arthrospira platensis commonly used as a blue-colorant in food. Color losses of phycocyanin can be reduced by electrostatic complexation with λ-carrageenan. The aim of this study was to investigate the effect of molecular weight (MW) of λ-carrageenan on the color stabilization of electrostatic complexes formed with phycocyanin and λ-carrageenan. Samples were heated to 70 or 90°C at pH 3.0 and stored at 25°C for 14 days. The MW of λ-carrageenan was reduced by ultrasound treatments for 15, 30, 60, and 90 min. Prolonged ultrasonication had a pronounced effect on the Mw, which decreased from 2,341 to 228 kDa (0–90 min). Complexes prepared with low MW λ-carrageenan showed greater color changes compared to complexes prepared with high MW λ-carrageenan. The MW had no visible effect on color stability on day 0, but green/yellow shifts were observed during storage and after heating to 70°C. Medium MW showed less color stabilization effects compared to low MW when heated to 70°C. Moreover, for solutions prepared with ultrasonicated λ-carrageenan, significant hue shifts toward green/yellow, and precipitation were observed after a heat treatment at 90°C. In addition, the sizes of the complexes were significantly reduced (646–102 nm) by using ultrasonicated λ-carrageenan, except for the lowest MW λ-carrageenan when heated to 90°C. Overall, these findings demonstrated that decreasing the MW of λC had adverse effects on the color stability of PC:λC complexes heated to 70 and 90°C.
Comparative Analysis of Elderly Patients Undergoing Radical Cystectomy With Ureterocutaneostomy or Ileal Conduit With a Special Focus on Bowl Complications Requiring Surgical Revision
Authors: Mally David , John Patricia , Pfister David , Heidenreich Axel , Albers Peter , Niegisch Günter
Published: June 2022

Objectives

Ileal conduits (ICs) carry an additional perioperative complication risk due to the bowel procedure. This analysis compares surgical outcomes in patients ≥75 years of age with ureterocutaneostomy (UCN) and IC after cystectomy (Cx).

Methods

Data of 527 patients included in a retrospective cystectomy database of two high volume centers (2008–2020) were queried to identify elderly patients (≥75 years) who underwent Cx either with IC or UCN. Patient characteristics of all patients [age, BMI, Charlson Comorbidity Index (CCI)], perioperative parameters (operation time, blood loss, transfusions, tumor stage), and postoperative complications (clavien >IIIA, intensive care unit (ICU) stay) were compared. As special focus, bowel complications requiring surgical revision (rBCs) were analyzed. In patients with IC, the rate of ureteral implantation stenosis (USt) was recorded. As a population of special interest, patients ≥80 years of age were analyzed separately. Categorical data were compared using Fisher exact test, and continuous data were compared using Mann–Whitney U test.

Results

A total of 163 patients ≥75 years of age (125 IC, 38 UCN) were identified. Patients with UCN were older and presented with a higher CCI, though differences were not statistically different. Surgery with palliative intent was more frequent in patients with UCN (37 vs. 10%). Operation time in UCN was significantly shorter (233 vs. 305 min, p = 0.02), while blood loss and transfusion rate were comparable. Overall complication rate (Clavien-Dindo grade IIIA–IVB) was comparable (UCN 34% vs. IC 37%). However, rBC was a rare complication in UCN (3/38) as compared to patients with IC (15/125). Frequency of postoperative ICU stay (UCN 16% vs. IC 16%) and 90-day mortality did not differ (UCN 3/38 patients, IC 5/125 patients). Regarding long-term follow-up, USt requiring revision or permanent stenting was seen in 18/125 (14%) patients with IC. In patients >80 years of age, results were comparable to the main cohort. Low event rate regarding complications and bias inherent of a retrospective analysis (selection bias, unequal distribution in case numbers) precludes detection of statistical differences regarding patients' characteristics and overall complication rate.

Conclusion

UCN is an alternative to IC in elderly and/or frail patients. Severe bowel complications are numerically less frequent and operation time is minimized.
Detection of Virulence-Associated Genes and in vitro Gene Transfer From Aeromonas sp. Isolated From Aquatic Environments of Sub-himalayan West Bengal
Authors: Mangar Preeti , Barman Partha , Kumar Anoop , Saha Aniruddha , Saha Dipanwita
Published: June 2022

Aeromonas is omnipresent in aquatic environments and cause disease within a wide host range. A total of thirty-four isolates from water samples of small fish farms were identified as Aeromonas based on biochemical characteristics and 16S rRNA gene sequence. A total of six virulent factors were analyzed which indicated 100% of isolates as beta-haemolytic and proteolytic, whereas 44.1, 38.2, and 70.6% of isolates produced DNAse, siderophore, and amylase, respectively. Studies on the occurrence of four genetic determinants of virulence factors revealed that aer/haem (haemolytic toxin) and flaA (polar flagella) genes were present in 44.1% of strains whereas ascV (type 3 secretion system) and aspA (serine protease) genes were detected in 21.5 and 8.82% of strains, respectively. Fish (Anabas testudineus) challenge studies showed that the isolate GP3 (Aeromonas veronii) bearing five virulent factors with the combination of aer/haem+/ascV+/fla+ genes induced severe lesions leading to 100% of mortality. In contrast, RB7 possessing four virulence factors and three genes (aer/haem+/ascV+/aspA+) could not produce severe lesions and any mortality indicating the absence of correlation between the virulence factors, its genes, and the pathogenicity in fishes. GP3 was cytotoxic to human liver cell line (WRL-68) in trypan blue dye exclusion assay. The 431 bp aer/haem gene of GP3 was transferable to E. coli Dh5α with a conjugational efficiency of 0.394 × 10–4 transconjugants per recipient cell. The transfer was confirmed by PCR and by the presence of 23-kb plasmids in both donor and transconjugants. Therefore, the occurrence of mobile genetic elements bearing virulence-associated genes in Aeromonas indicates the need for periodic monitoring of the aquatic habitat to prevent disease outbreaks.
Public Understanding of Adolescents’ Risks on Facebook in Taiwan
Authors: Tan Yue
Published: June 2022

An increasing number of parents and scholars have begun expecting schools and the government to share the responsibility of reducing the potential negative effects of SNS use among adolescents. This study examines how the public understands the risks that adolescents face, as well as the causes and solutions, and how news media influence not only the public’s risk perceptions but also their policy preference for public interventions. Drawing on framing and attribution theories, this study used two datasets. First, the content analysis data explore Taiwanese news media’s coverage of youths’ online behaviors and how the media has framed the question “Who is responsible for adolescents’ risky and opportunity behaviors?” Second, the public opinion survey data addresses the influence of news consumption on the public perception of the risks facing adolescent Facebook users, the public’s attribution of related responsibilities to various stakeholders, and the public’s evaluation of parental mediation and government regulations.
Mitochondrial Dysfunction and the Glycolytic Switch Induced by Caveolin-1 Phosphorylation Promote Cancer Cell Migration, Invasion, and Metastasis
Authors: Díaz-Valdivia Natalia , Simón Layla , Díaz Jorge , Martinez-Meza Samuel , Contreras Pamela , Burgos-Ravanal Renato , Pérez Viviana I. , Frei Balz , Leyton Lisette , Quest Andrew F. G.
Published: June 2022

Cancer cells often display impaired mitochondrial function, reduced oxidative phosphorylation, and augmented aerobic glycolysis (Warburg effect) to fulfill their bioenergetic and biosynthetic needs. Caveolin-1 (CAV1) is a scaffolding protein that promotes cancer cell migration, invasion, and metastasis in a manner dependent on CAV1 phosphorylation on tyrosine-14 (pY14). Here, we show that CAV1 expression increased glycolysis rates, while mitochondrial respiration was reduced by inhibition of the mitochondrial complex IV. These effects correlated with increased reactive oxygen species (ROS) levels that favored CAV1-induced migration and invasion. Interestingly, pY14-CAV1 promoted the metabolic switch associated with increased migration/invasion and augmented ROS-inhibited PTP1B, a phosphatase that controls pY14 levels. Finally, the glycolysis inhibitor 2-deoxy-D-glucose reduced CAV1-enhanced migration in vitro and metastasis in vivo of murine melanoma cells. In conclusion, CAV1 promotes the Warburg effect and ROS production, which inhibits PTP1B to augment CAV1 phosphorylation on tyrosine-14, thereby increasing the metastatic potential of cancer cells.
Super-Enhancers, Phase-Separated Condensates, and 3D Genome Organization in Cancer
Authors: Tang Seng Chuan , Vijayakumar Udhaya , Zhang Ying , Fullwood Melissa Jane
Published: June 2022

3D chromatin organization plays an important role in transcription regulation and gene expression. The 3D genome is highly maintained by several architectural proteins, such as CTCF, Yin Yang 1, and cohesin complex. This structural organization brings regulatory DNA elements in close proximity to their target promoters. In this review, we discuss the 3D chromatin organization of super-enhancers and their relationship to phase-separated condensates. Super-enhancers are large clusters of DNA elements. They can physically contact with their target promoters by chromatin looping during transcription. Multiple transcription factors can bind to enhancer and promoter sequences and recruit a complex array of transcriptional co-activators and RNA polymerase II to effect transcriptional activation. Phase-separated condensates of transcription factors and transcriptional co-activators have been implicated in assembling the transcription machinery at particular enhancers. Cancer cells can hijack super-enhancers to drive oncogenic transcription to promote cell survival and proliferation. These dysregulated transcriptional programs can cause cancer cells to become highly dependent on transcriptional regulators, such as Mediator and BRD4. Moreover, the expression of oncogenes that are driven by super-enhancers is sensitive to transcriptional perturbation and often occurs in phase-separated condensates, supporting therapeutic rationales of targeting SE components, 3D genome organization, or dysregulated condensates in cancer.
Effects of the Deposition Mode and Heat Treatment on the Microstructure and Wettability of Y2O3 Coatings Prepared by Reactive Magnetron Sputtering
Authors: Ma Xiaorui , Huang Zeyi , Feng Lin
Published: June 2022

A robust hydrophobic Y2O3 coating at high temperatures is important for industrial applications. In this study, Y2O3 thin films on Si substrates were prepared by reactive direct current magnetron sputtering. By changing the deposition power, Y2O3 thin films with different microstructures were obtained in poison mode and metallic mode, respectively. In order to understand the effect of heat treatment on the microstructure and hydrophobicity of Y2O3, the samples were annealed at 400 °C in the air. Compared to metallic mode, no crack was formed on the surface of the Y2O3 film prepared in poison mode. In addition, the water contact angle on the surface of the Y2O3 thin film deposited in poison mode was above 90° before and after annealing at 400 °C. It has been demonstrated that the initial high concentration of physically absorbed oxygen and its slow desorption process in a Y2O3 thin film prepared in poison mode contributes to the hydrophobicity of the thin film at high temperatures. These results can provide insights into the large-scale fabrication of hydrophobic Y2O3 coatings for high-temperature applications.
Growing Teratoma Syndrome in the Setting of Sarcoidosis: A Case Report and Literature Review
Authors: Shahnam Adel , Sayer Robyn , Herbst Unine , Sharma Raghwa , Yoon Won-hee , Dinihan Tim , Gao Bo
Published: June 2022

Growing teratoma syndrome (GTS) is rare and can mimic disease recurrence in patients with a history of immature teratoma. Benign hypermetabolic lymphadenopathy found on staging and surveillance computed tomography (CT) and positron emission tomography (PET) may lead to the presumption of metastatic malignancy. We report a case of a 38 year old with mixed mature and immature teratomas who developed new peritoneal masses after adjuvant chemotherapy despite a normalization of tumor markers. In addition to low FDG uptake observed in these peritoneal masses, a PET scan showed hypermetabolic lymphadenopathy and pulmonary and spleen lesions suggesting widespread metastases. Subsequent surgical resection confirmed a mixed pathology with GTS and sarcoidosis. We reviewed the current literature evidence of GTS and sarcoidosis as a benign cause of lymphadenopathy in cancer patients. We emphasize the importance of a tissue diagnosis before instituting therapy for presumed cancer recurrence to avoid potentially fatal diagnostic traps and management errors. A multiple disciplinary team approach is imperative in managing patients with suspected recurrent immature teratomas.
Current and Future Biomarkers for Immune Checkpoint Inhibitors in Head and Neck Squamous Cell Carcinoma
Authors: Park Jong Chul , Krishnakumar Hari N. , Saladi Srinivas Vinod
Published: June 2022

With the introduction of immunotherapy, significant improvement has been made in the treatment of head and neck squamous cell carcinoma (HNSCC). However, only a small subset of patients with HNSCC benefit from immunotherapy. The current biomarker, a programmed cell death protein ligand 1 (PD-L1) expression that is widely used in treatment decision making for advanced HNSCC, has only a moderate predictive value. Additionally, PD-L1-based assay has critical inherent limitations due to its highly dynamic nature and lack of standardization. With the advance in molecular techniques and our understanding of biology, more reliable, reproducible, and practical novel biomarkers are being developed. These include but are not limited to neoantigen/mutation characteristics, immune transcriptomes, tumor-infiltrating immune cell composition, cancer epigenomic, proteomics and metabolic characteristics, and plasma-based and organoid assays.
P2P Lending Default Prediction Based on AI and Statistical Models
Authors: Ko Po-Chang , Lin Ping-Chen , Do Hoang-Thu , Huang You-Fu
Published: June 2022

Peer-to-peer lending (P2P lending) has proliferated in recent years thanks to Fintech and big data advancements. However, P2P lending platforms are not tightly governed by relevant laws yet, as their development speed has far exceeded that of regulations. Therefore, P2P lending operations are still subject to risks. This paper proposes prediction models to mitigate the risks of default and asymmetric information on P2P lending platforms. Specifically, we designed sophisticated procedures to pre-process mass data extracted from Lending Club in 2018 Q3–2019 Q2. After that, three statistical models, namely, Logistic Regression, Bayesian Classifier, and Linear Discriminant Analysis (LDA), and five AI models, namely, Decision Tree, Random Forest, LightGBM, Artificial Neural Network (ANN), and Convolutional Neural Network (CNN), were utilized for data analysis. The loan statuses of Lending Club’s customers were rationally classified. To evaluate the models, we adopted the confusion matrix series of metrics, AUC-ROC curve, Kolmogorov–Smirnov chart (KS), and Student’s t-test. Empirical studies show that LightGBM produces the best performance and is 2.91% more accurate than the other models, resulting in a revenue improvement of nearly USD 24 million for Lending Club. Student’s t-test proves that the differences between models are statistically significant.
A Fisher Information-Based Incompatibility Criterion for Quantum Channels
Authors: Zhang Qing-Hua , Nechita Ion
Published: June 2022

We introduce a new incompatibility criterion for quantum channels based on the notion of (quantum) Fisher information. Our construction is based on a similar criterion for quantum measurements put forward by H. Zhu. We then study the power of the incompatibility criterion in different scenarios. First, we prove the first analytical conditions for the incompatibility of two Schur channels. Then, we study the incompatibility structure of a tuple of depolarizing channels, comparing the newly introduced criterion with the known results from asymmetric quantum cloning.
Comprehensive Vulnerability Assessment of Urban Areas Using an Integration of Fuzzy Logic Functions: Case Study of Nasiriyah City in South Iraq
Authors: Hanoon Sadeq Khaleefah , Abdullah Ahmad Fikri , Shafri Helmi Z. M. , Wayayok Aimrun
Published: June 2022

Globally, urbanisation has been the most significant factor causing land use and land cover changes due to accelerated population growth and limited governmental regulation. Urban communities worldwide, particularly in Iraq, are on the frontline for dealing with threats associated with environmental degradation, climate change and social inequality. However, with respect to the effects of urbanization, most previous studies have overlooked ecological problems, and have disregarded strategic environmental assessment, which is an effective tool for ensuring sustainable development. This study aims to provide a comprehensive vulnerability assessment model for urban areas experiencing environmental degradation, rapid urbanisation and high population growth, to help formulate policies for urban communities and to support sustainable livelihoods in Iraq and other developing countries. The proposed model was developed by integrating three functions of fuzzy logic: the fuzzy analytic hierarchy process, fuzzy linear membership and fuzzy overlay gamma. Application of the model showed that 11 neighbourhoods in the study area, and more than 175,000 individuals, or 25% of the total population, were located in very high vulnerability regions. The proposed model offers a decision support system for allocating required financial resources and efficiently implementing mitigation processes for the most vulnerable urban areas.