Functional connectomics spanning multiple areas of mouse visual cortex
Authors:   The MICrONS Consortium, Bae J. Alexander , Baptiste Mahaly , Bishop Caitlyn A. , Bodor Agnes L. , Brittain Derrick , Buchanan JoAnn , Bumbarger Daniel J. , Castro Manuel A. , Celii Brendan , Cobos Erick , Collman Forrest , da Costa Nuno Maçarico , Dorkenwald Sven , Elabbady Leila , Fahey Paul G. , Fliss Tim , Froudarakis Emmanouil , Gager Jay , Gamlin Clare , Gray-Roncal William , Halageri Akhilesh , Hebditch James , Jia Zhen , Joyce Emily , Joyce Justin , Jordan Chris , Kapner Daniel , Kemnitz Nico , Kinn Sam , Kitchell Lindsey M. , Koolman Selden , Kuehner Kai , Lee Kisuk , Li Kai , Lu Ran , Macrina Thomas , Mahalingam Gayathri , Matelsky Jordan , McReynolds Sarah , Miranda Elanine , Mitchell Eric , Mondal Shanka Subhra , Moore Merlin , Mu Shang , Muhammad Taliah , Nehoran Barak , Ogedengbe Oluwaseun , Papadopoulos Christos , Papadopoulos Stelios , Patel Saumil , Pitkow Xaq , Popovych Sergiy , Ramos Anthony , Clay Reid R. , Reimer Jacob , Rivlin Patricia K. , Rose Victoria , Schneider-Mizell Casey M. , Seung H. Sebastian , Silverman Ben , Silversmith William , Sterling Amy , Sinz Fabian H. , Smith Cameron L. , Suckow Shelby , Takeno Marc , Tan Zheng H. , Tolias Andreas S. , Torres Russel , Turner Nicholas L. , Walker Edgar Y. , Wang Tianyu , Wanner Adrian , Wester Brock A. , Williams Grace , Williams Sarah , Willie Kyle , Willie Ryan , Wong William , Wu Jingpeng , Xu Chris , Yang Runzhe , Yatsenko Dimitri , Ye Fei , Yin Wenjing , Young Rob , Yu Szi-chieh , Xenes Daniel , Zhang Chi 
Published: July 2021

To understand the brain we must relate neurons’ functional responses to the circuit architecture that shapes them. Here, we present a large functional connectomics dataset with dense calcium imaging of a millimeter scale volume. We recorded activity from approximately 75,000 neurons in primary visual cortex (VISp) and three higher visual areas (VISrl, VISal and VISlm) in an awake mouse viewing natural movies and synthetic stimuli. The functional data were co-registered with a volumetric electron microscopy (EM) reconstruction containing more than 200,000 cells and 0.5 billion synapses. Subsequent proofreading of a subset of neurons in this volume yielded reconstructions that include complete dendritic trees as well the local and inter-areal axonal projections that map up to thousands of cell-to-cell connections per neuron. Here, we release this dataset as an open-access resource to the scientific community including a set of tools that facilitate data retrieval and downstream analysis. In accompanying papers we describe our findings using the dataset to provide a comprehensive structural characterization of cortical cell types1–3and the most detailed synaptic level connectivity diagram of a cortical column to date2, uncovering unique cell-type specific inhibitory motifs that can be linked to gene expression data4. Functionally, we identify new computational principles of how information is integrated across visual space5, characterize novel types of neuronal invariances6and bring structure and function together to decipher a general principle that wires excitatory neurons within and across areas7, 8.