bluuebunny/crossref_metadata_2025 huggingface dataset:
Automatic Optimization of Input Split and Bias Voltage in Digitally Controlled Dual-Input Doherty RF PAs
Authors: Mengozzi Mattia , Gibiino Gian Piero , Angelotti Alberto Maria , Santarelli Alberto , Florian Corrado , Colantonio Paolo
Published: July 2022

Digitally controlled Dual-Input Doherty Power Amplifiers (DIDPAs) are becoming increasingly popular due to the flexible input signal splitting between the main and auxiliary stages. Nevertheless, the presence of many degrees of freedom, e.g., input amplitude split and phase displacement as well as biasing for multiple stages, often involves inefficient trial-and-error procedures to reach a suitable PA performance. This article presents automated parameter setting based on coordinate descent or Bayesian optimizations, demonstrating an improvement in the performance in terms of RF output power and power-added efficiency (PAE) in the presence of broadband-modulated signals, yet maintaining suitable linear behavior for, e.g., communications applications.
Monitoring Metal Wear Particles of Friction Pairs in the Oil Systems of Gas Turbine Power Plants
Authors: Belopukhov Valentin , Blinov Andrey , Borovik Sergey , Luchsheva Mariya , Muhutdinov Farit , Podlipnov Petr , Sazhenkov Aleksey , Sekisov Yuriy
Published: July 2022

In the example of the aviation gas turbine engine the problem of monitoring metal wear particles of friction pairs in the oil systems of gas turbine power plants is considered. The solution based on using the multi-channel cluster single-coil eddy current sensor (CSCECS) with sensitive elements in the form of single circuits is proposed. The CSCECS provides the detection of ferromagnetic and non-ferromagnetic particles and their ranking by several size groups. The sensor is invariant to the size (inner diameter) of the monitored oil pipeline and has high throughput and identical sensitivity across all channels. Two variants of the hardware structure of the debris continuous monitoring system (DCMS) prototype implementing the proposed approach are suggested. The first variant is intended for engine bench tests and contains the CSCECS with integrated preamplifiers and forced air cooling of the electronic modules. The second variant of the DCMS prototype involves the use of the uncooled sensors without built-in electronics and it focuses on operation in autonomous mode not only in bench tests but also during the engine normal operation. A brief description of the DCMS operational algorithm is given. The algorithm is the same for both hardware versions but differs at the software implementation level. The correctness of the algorithm for the detection and size identification of the wear metal particles was verified during the laboratory experiments with a total duration of 5 h and 30 min. The DCMS prototype was also examined during the full-scale engine bench tests. The experiments indicated that the number, size, and magnetic properties of the particles detected by DCMS generally corresponded to the number, size, and magnetic properties of the particles fixed by the MetalSCAN oil debris monitoring system which was used for verification of the DCMS functional capability. The results were also confirmed through laboratory analysis of the wipe samples on the debris filters. However, unlike the existing approaches, the design of the CSCECS additionally made it possible to evaluate the oil flow features in the pipeline of the engine lubrication system.
Wood Biomass Resources in Poland Depending on Forest Structure and Industrial Processing of Wood Raw Material
Authors: Wieruszewski Marek , Górna Aleksandra , Mydlarz Katarzyna , Adamowicz Krzysztof
Published: July 2022

(1) Wood is a widely available raw material on the market, which satisfies the industrial demand and which is used both as a source of biomass for the wood materials industry in a broad sense and for energy-supplying purposes. These areas prove the functional values and the possibilities of the directional use of low-quality wood products. One of the factors influencing the overall balance of the wood biomass is the form and quality of the wood material that cannot be further processed mechanically. This study was conducted to determine the influence of this material by presenting the dependence between the level of wood biomass resources and the conditions of wood acquisition and processing in Poland. (2) The basic directions of biomass acquisition were verified in correlation with the level of its acquisition from forest areas and with the form of by-products generated by sawmills. The research was based on the data from reference publications and analysis of the processing of raw wood in sawmills. The research was conducted on raw hardwood and softwood from coniferous and deciduous forests in Poland. (3) The research confirmed the influence of the processing method on the form and share of by-products. It also showed that the form of the wood biomass obtained was influenced by the region of Poland. (4) The research showed that the regionalisation and wood processing directions were correlated with the structure of the wood biomass acquired.
Adult Presentation of X-Linked Hypophosphatemia
Authors: Ito Nobuaki
Published: July 2022

Adult X-linked hypophosphatemia (XLH) patients present with specific symptoms, including enthesopathies (e.g., ossification of longitudinal ligaments (OPLL), osteophytes around large joints, and enthesopathy in the Achilles tendons), early osteoarthritis, the development of severe secondary and tertiary hyperparathyroidism (SHPT/THPT), and the subsequent progression of chronic kidney disease (CKD). In addition, these patients exhibit the typical phenotypes of osteomalacia, such as pseudofracture and fracture in weight-bearing bones, odontitis, and tooth abscesses. The mechanism underlying enthesopathy development is unknown; however, a common underlying mechanism among XLH and autosomal recessive hypophosphatemic rickets (ARHR1/2) due to mutations in PHEX, DMP1, and ENPP1 is assumed. Clarification of the pathogenesis and drug discovery for this complication is an urgent issue, as many adult XLH patients suffer subsequent debilitating nervous symptoms or impingement syndrome, and existing treatments are ineffective. Severe SHPT and THPT are associated with conventional therapy, including active vitamin D and phosphate supplementation, and complicated and careful adjustment of dosages by experienced clinicians is required to avoid SHPT/THPT. Burosumab is a very effective therapy without risk for the development of SHPT/THPT. However, indications for this drug should be carefully considered, along with cost-effectiveness, guidelines or recommendations, and the health care system of each country.
The Old and the New in Subacute Thyroiditis: An Integrative Review
Authors: Lanzo Nicola , Patera Bohdan , Fazzino Gaia , Gallo Daniela , Lai Adriana , Piantanida Eliana , Ippolito Silvia , Tanda Maria
Published: July 2022

Subacute thyroiditis (SAT) is the most common cause of neck pain and thyrotoxicosis. Although this disease was recognized already by the end of the 18th century, new concepts regarding pathogenesis have emerged in recent years. Moreover, in the last two years, literature on SAT has increased significantly due to articles describing the possible connection with coronavirus disease 2019 (COVID-19). This integrative review depicts old and new concepts of this disease, proposing a detailed overview of pathogenesis, a practical approach to diagnosis and treatment, and a thorough description of the latest discoveries regarding the association of SAT with COVID-19.
Evaluation of the Technological Properties of Soil–Cement Bricks with Incorporation of Coconut Fiber Powder
Authors: Ferreira Gabriela Machado Guimarães , Cecchin Daiane , Valadão Izabella Christynne Ribeiro Pinto , da Silva Tulane Rodrigues , do Carmo Dirlane de Fátima , Moll Hüther Cristina , Ferreira Flávio , de Azevedo Afonso Rangel Garcez
Published: July 2022

The objective of this work was to evaluate the effect of partial replacement of soil by different percentages of coconut fiber powder in the manufacture of soil–cement bricks. The reference mix ratio of 10:1 (soil:cement) in volume was used for the manufacture of bricks, in addition to the partial replacement of soil mass by 5, 10, and 15% of coconut fiber. The characterization of the raw materials was performed with the analysis of the granulometry, together with technological tests, such as mechanical compressive strength and water absorption. As a result, it was observed that the soil has 34.30% clay and 62.80% sand, characterizing a sandy-clay soil type and the coconut fiber powder was characterized as a fine aggregate. The mechanical compressive strength tests showed a decrease in their average values according to the increase in the incorporation of coconut fiber into the bricks. It was concluded that the results of the mechanical compressive strength and some of the water absorption are in disagreement with the Brazilian technical standard. This conclusion corroborates other studies that show the difficulty in standardizing reference mixtures and working with soil, which is a highly heterogeneous material.
Bactericidal Properties of Low-Density Polyethylene (LDPE) Modified with Commercial Additives Used for Food Protection in the Food Industry
Authors: Janczak Katarzyna , Bajer Krzysztof , Malinowski Rafał , Wedderburn Lauren , Kosmalska Daria , Królikowski Bogusław
Published: July 2022

This study investigated the influence of commercially available food preservatives: Natamax® (containing natamycin) and Nisaplin® (containing nisin) on the antimicrobial properties of LDPE film, commonly used for food packaging. Studies have shown that the addition of 3% Natamax® or, alternatively, the addition of 5% Nisaplin® provides an LDPE film with effective antimicrobial protection. The applied biocides did not significantly affect the strength and rheological properties of LDPE. However, differences in optical properties were observed. The transparency of the samples decreased slightly with the addition of 3% or 5% Natamax® (by approx. 1% and 3%, respectively). A significant change was observed in the film haze, the addition of 5% Natamax® increased this parameter by approx. 80%, while 5% Nisaplin® increased it by approx. 19%. Both Natamax® and Nisaplin® agents can be successfully used to manufacture food packaging materials with antimicrobial protection. Natamax® showed a stronger bactericidal effect, while Nisaplin® changed other properties less significantly.
Landslide Susceptibility Research Combining Qualitative Analysis and Quantitative Evaluation: A Case Study of Yunyang County in Chongqing, China
Authors: Zhang Wengang , Liu Songlin , Wang Luqi , Samui Pijush , Chwała Marcin , He Yuwei
Published: July 2022

Machine learning-based methods are commonly used for landslide susceptibility mapping. Most of the recent publications focused on quantitative analysis, i.e., improving data processing methods, comparing and perfecting the data-driven model itself, but rarely taking the qualitative aspects of the local landslide occurrences into consideration and the further analysis of the key features was always lacking. This study aims to combine qualitative and quantitative analysis and examine its effect on mapping accuracy; based on the feature importance ranks and the related literature, the key features for identifying landslide/non-landslide points of different sub-zones were further analyzed. Before modeling, the study area Yunyang County, Chongqing City, China, was manually divided into four sub-zones based on the information from geological hazards exploration in Chongqing, including the mechanism of landslide formation and sliding failure and geomorphic unit characteristics. Upon the qualitative analysis basis, five grid searches tuned random forest models (one for the whole region and four for the sub-zones independently) were established by 1654 data points and 20 conditioning features. Compared with the conventional data-driven method, the integrated quantitative evaluation based on the qualitative analysis results showed higher reliability, which not only improved the mapping accuracy but also increased the AUC values of all four sub-models, which were 8.8%, 2.3%, 1.9% and 9.1% higher than that of the parent model. Moreover, the quantitative evaluation based on the qualitative analysis revealed the key factors affecting local landslide formation. Therefore, qualitative analysis is recommended in future landslide susceptibility modeling with the additional combination of data-driven methods.
Simple Synthesis of Fe3O4@-Activated Carbon from Wastepaper for Dispersive Magnetic Solid-Phase Extraction of Non-Steroidal Anti-Inflammatory Drugs and Their UHPLC–PDA Determination in Human Plasma
Authors: Ferrone Vincenzo , Bruni Pantaleone , Canale Valentino , Sbrascini Leonardo , Nobili Francesco , Carlucci Giuseppe , Ferrari Stefania
Published: July 2022

In the present society, the recycling and reuse of valuable substances are of utmost importance for economic and environmental purposes. At the same time, there is a pressing need to develop new methods to protect the ecosystem from many human activities, including those that have contributed to an ever-increasing presence of pharmaceutical pollutants. In this study, a straightforward approach that applies a magnetic carbon composite for the effective removal of NSAIDs from biological fluids is reported. The composite was produced by recycling wasted handkerchiefs, to provide cellulose to the reactive system and then transformed into carbon via calcination at high temperature. The morphological and structural features of the prepared “Fe3O4@-activated carbon” samples were investigated via thermal analysis, X-ray diffraction, Raman spectroscopy, and scanning electron microscopy. Magnetic solid-state extraction was carried out to reveal the adsorption capabilities of the magnetic carbon composite and then combined with UHPLC–PDA for the determination and quantification of five NSAIDs (furprofen, indoprofen, ketoprofen, flurbiprofen, and indomethacin). The method developed herein proved to be fast and accurate. The adsorbent could be reused for up to 10 cycles, without any decrease in performance; thus, it contributes to an intelligent and sustainable economic strategy projected toward minimal waste generation.
Identification of Proteins Responsible for High Activity of Cysteine Proteinase Inhibitor in the Blood of Nile Tilapia Oreochromis niloticus
Authors: Mun Seong Hee , Kwon Joon Yeong
Published: July 2022

Cysteine proteinase inhibitors (CPIs) protect tissues and organs against cysteine proteinases in animal blood and have attracted much attention for use in food processing and medical sciences for humans and animals. Several CPI proteins, which include stefins, cystatins, kininogens, histidine-rich glycoproteins (HRG) and fetuins, have been identified and characterized in mammals. Fish blood also contains high CPI activity, but the identity of the major protein responsible for this activity has not been clarified. This study was conducted to screen CPI activity by examining papain inhibitory activity from various different tissues in Nile tilapia Oreochromis niloticus and to identify major proteins for the activity in the blood. CPI activity was highest in the serum among the tissues screened in this study (at least fourfold higher than in other tissues)(P < 0.05). Major proteins for CPI activity in serum were purified using a CNBr-activated sepharose 4B column, gel filtration and an ion exchange FPLC column. From these purifications, two proteins with strong CPI activity were isolated and partially sequenced. Based on their molecular weights and partial amino sequences, the two major proteins with CPI activity from the blood in this species were found to be fetuin B (60 kDa) and kininogen (54 kDa).
Development of a New Strategy for Studying the Oxygen Consumption Potential of Wine through the Grape Extract Evaluation
Authors: Carrasco-Quiroz Marioli , Martínez-Gil Ana María , Nevares Ignacio , Martínez-Martínez Víctor , Sánchez-Gómez Rosario , del Alamo-Sanza Maria
Published: July 2022

The development of a method to determine the aging potential of wine at the time of harvest, through the evaluation of its oxygen avidity, is a potential tool for the winemaking sector. To this end, it is necessary to formulate a potential wine with this grape prior to alcoholic fermentation. The main objective of this method was to optimize a formulation of the potential wine, based on the grape extracts (GEs), to subsequently evaluate its oxygen consumption kinetics, guaranteeing maximum differentiation between the different GEs. The optimization was carried out with a Taguchi orthogonal matrix design, which optimized the variables to be used in the GE reconstitution. The variables studied were pH, Fe2+, Cu2+, Mn2+, alcohol content and acetaldehyde. The evaluation of the characteristic parameters of the consumption kinetics of each of the GEs allowed us to know the different reconstitution conditions that most influence the differentiation of the oxygen consumption kinetics of very similar GEs. The reconstitution conditions chosen were pH 3.3; 1 mg/L Fe2+; 0.1 mg/L Cu2+; 1 mg/L Mn2+; 12% (v/v) alcoholic strength and 10 mg/L acetaldehyde, with pH, Fe2+ and Mn2+ being the significant conditions. The kinetics of reconstituted GE could be a tool for the classification and evaluation of grapes according to their aging potential or shelf life of the wine made.
Chemical Characterization, α-Glucosidase, α-Amylase and Lipase Inhibitory Properties of the Australian Honey Bee Propolis
Authors: Uddin Sabah , Brooks Peter R. , Tran Trong D.
Published: July 2022

The use of functional foods and nutraceuticals as a complementary therapy for the prevention and management of type 2 diabetes and obesity has steadily increased over the past few decades. With the aim of exploring the therapeutic potentials of Australian propolis, this study reports the chemical and biological investigation of a propolis sample collected in the Queensland state of Australia which exhibited a potent activity in an in vitro α-glucosidase inhibitory screening. The chemical investigation of the propolis resulted in the identification of six known prenylated flavonoids including propolins C, D, F, G, H, and solophenol D. These compounds potently inhibited the α-glucosidase and two other enzymes associated with diabetes and obesity, α-amylase, and lipase on in vitro and in silico assays. These findings suggest that this propolis is a potential source for the development of a functional food to prevent type 2 diabetes and obesity. The chemical analysis revealed that this propolis possessed a chemical fingerprint relatively similar to the Pacific propolis found in Okinawa (South of Japan), Taiwan, and the Solomon Islands. This is the first time the Pacific propolis has been identified in Australia.
Bioaccessibility of Macrominerals and Trace Elements from Tomato (Solanum lycopersicum L.) Farmers’ Varieties
Authors: Ciudad-Mulero María , Pinela José , Carvalho Ana Maria , Barros Lillian , Fernández-Ruiz Virginia , Ferreira Isabel C. F. R. , Sánchez-Mata María de Cortes , Morales Patricia
Published: July 2022

Traditional farmers’ varieties of tomato grown under extensive farming techniques are considered delicious and healthy foods and are preferred by local consumers. Tomatoes are an important component of a healthy diet, as they provide essential micronutrients, including minerals, which are vital to healthy development, disease prevention, and wellbeing. Given the considerable dietary intake of tomatoes and the scarcity of information about the bioaccessibility of inorganic constituents in this fruit, this study was carried out to evaluate the content and bioaccessibility of minerals (macro- and microelements) in tomato farmers’ varieties widely cultivated in northeastern Portugal homegardens. Among the macroelements, K stood out as the most abundant mineral in the studied varieties, followed by Mg, Ca, and Na. Regarding the microelements, while the yellow tomato had higher concentrations of Fe and Cu, the round tomato had more Zn and Mn. The in vitro bioaccessibility assessment showed that, among the macroelements, Mg was more bioaccessible than Ca and K when all the tomato varieties were considered together. Among the microelements, Cu seemed to be the most bioaccessible. Although the contribution of a 100 g serving of the studied tomato farmers’ varieties to the dietary reference intakes (DRIs) of minerals is relatively low, this food could contribute to reaching these mineral requirements, as it is included in the diet of most of the population, especially in Mediterranean regions.
Pasteurization Modifies the Sensorial Attributes and Nutritional Profile of Orange Pulp By-Product
Authors: Giavoni Marta , Villanueva-Suárez María José , De la Peña-Armada Rocío , Garcia-Alonso Alejandra , Mateos-Aparicio Inmaculada
Published: July 2022

After orange processing, different by-products are generated, i.e., peels, seeds and pulps. The pulp is highly perishable, being an unstable food matrix that needs a preservation process to be stored and used again in the food production chain. Pasteurization is the technique of choice before aseptically packaging and storing under refrigerated conditions. In this study, the effect of pasteurization has been evaluated on the chemical, functional and sensorial profiles. Ash content decreased (p < 0.05) after the thermal treatment. Indeed, magnesium, calcium and zinc diminished, although copper was found to be higher (p < 0.05) in the pasteurized product. Total dietary fiber decreased (p < 0.05), but soluble dietary fiber raised (p < 0.05) due to hydrolysis caused by pasteurization. SDF:IDF ratio, hydration properties, and fat binding capacity were improved. Total soluble phenolic compounds remained similar but FRAP and DPPH scavenging activity decreased (p < 0.05) in the pasteurized by-product. Regarding the sensorial profile, pasteurization produced darkening, appearance of a cooked smell and an increase in bitterness. Therefore, pasteurization deteriorates the sensorial profile being able to change the attributes of an added-pasteurized-pulp juice; however, it is a good choice to preserve the orange pulp by-product to formulate food products different from juices or other beverages.
The Use of Hemispherical Directional Reflectance to Evaluate the Interaction of Food Products with Radiation in the Solar Spectrum
Authors: Błoński Bartosz , Wilczyński Sławomir , Hartman-Petrycka Magdalena , Michalecki Łukasz
Published: July 2022

Food product packaging should block light to protect nutrients, color and active ingredients in functional food from degradation. Currently, packages are not optimized in terms of the solar radiation impact on the products they contain. The aim of this study was to develop a method of quantifying the interaction of food products with solar radiation, which would enable the optimization and selection of packaging that would protect the product from the spectral range specifically absorbed by it. In order to determine the reflectance of chocolate, the total reflectance ratio was measured. For this purpose, a SOC 410 Solar DHR reflectometer from Surface Optics Corporation, San Diego, CA, USA was used. Directional reflectance was measured for seven discrete spectral ranges from 335 to 2500 nm, which correspond to the spectrum of solar radiation. The value of total reflectance for chocolate differed significantly in the studied spectral ranges. The highest reflectance ratio, averaged for all the tested chocolate, was recorded for the spectral range 700–1100 nm and the lowest for the 335–380 nm range. The total reflectance was significantly correlated with the cocoa content and the brightness of the chocolate. The proposed method of hemispheric directional reflectance enables the measurement of the total reflectance of food products. It can be used as a measure of exposure to radiation. Thus, it is possible to design a package that will protect the product from the spectral range that is most harmful for it.
Use of Large-Scale Genomics to Identify the Role of Animals and Foods as Potential Sources of Extraintestinal Pathogenic Escherichia coli That Cause Human Illness
Authors: Harrison Lucas , Tyson Gregory H. , Strain Errol , Lindsey Rebecca L. , Strockbine Nancy , Ceric Olgica , Fortenberry Gamola Z. , Harris Beth , Shaw Sheryl , Tillman Glenn , Zhao Shaohua , Dessai Uday
Published: July 2022

Extraintestinal pathogenic Escherichia coli (ExPEC) cause urinary tract and potentially life-threatening invasive infections. Unfortunately, the origins of ExPEC are not always clear. We used genomic data of E. coli isolates from five U.S. government organizations to evaluate potential sources of ExPEC infections. Virulence gene analysis of 38,032 isolates from human, food animal, retail meat, and companion animals classified the subset of 8142 non-diarrheagenic isolates into 40 virulence groups. Groups were identified as low, medium, and high relative risk of containing ExPEC strains, based on the proportion of isolates recovered from humans. Medium and high relative risk groups showed a greater representation of sequence types associated with human disease, including ST-131. Over 90% of food source isolates belonged to low relative risk groups, while >60% of companion animal isolates belonged to medium or high relative risk groups. Additionally, 18 of the 26 most prevalent antimicrobial resistance determinants were more common in high relative risk groups. The associations between antimicrobial resistance and virulence potentially limit treatment options for human ExPEC infections. This study demonstrates the power of large-scale genomics to assess potential sources of ExPEC strains and highlights the importance of a One Health approach to identify and manage these human pathogens.
Is the Probability of Tossing a Coin Really 50–50%? Part 1: Static Model and Dynamic Models without Rebounds
Authors: Pletser Vladimir
Published: July 2022

Considering that a fair coin has two sides and a cylindrical edge, the probability that it would fall on its edge is calculated, yielding the probability of heads or tails of less than 50%. In this first part, the theoretical models for a static case and for five dynamic cases, without rebounds, show that there is a small probability that the coin does not fall on its head or tail, depending on the initial toss conditions, the coin geometry and conditions of the coin and landing surfaces. It is found that the probability that a 50 Eurocent coin thrown from a normal height with common initial velocity conditions and appropriate surface conditions will end up on its edge is in the order of one against several thousand. In the second part of the paper, the dynamic model with rebounds is investigated.
Generalized Navier–Stokes Equations with Non-Homogeneous Boundary Conditions
Authors: Baranovskii Evgenii S. , Artemov Mikhail A.
Published: July 2022

We study the generalized unsteady Navier–Stokes equations with a memory integral term under non-homogeneous Dirichlet boundary conditions. Using a suitable fractional Sobolev space for the boundary data, we introduce the concept of strong solutions. The global-in-time existence and uniqueness of a small-data strong solution is proved. For the proof of this result, we propose a new approach. Our approach is based on the operator treatment of the problem with the consequent application of a theorem on the local unique solvability of an operator equation involving an isomorphism between Banach spaces with continuously Fréchet differentiable perturbations.
Numerical Approximation of the Fractional Rayleigh–Stokes Problem Arising in a Generalised Maxwell Fluid
Authors: Long Le Dinh , Moradi Bahman , Nikan Omid , Avazzadeh Zakieh , Lopes António M.
Published: July 2022

This paper presents a numerical technique to approximate the Rayleigh–Stokes model for a generalised Maxwell fluid formulated in the Riemann–Liouville sense. The proposed method consists of two stages. First, the time discretization of the problem is accomplished by using the finite difference. Second, the space discretization is obtained by means of the predictor–corrector method. The unconditional stability result and convergence analysis are analysed theoretically. Numerical examples are provided to verify the feasibility and accuracy of the proposed method.
Fractal Operators and Fractional Dynamics with 1/2 Order in Biological Systems
Authors: Yin Yajun , Guo Jianqiao , Peng Gang , Yu Xiaobin , Kong Yiya
Published: July 2022

This paper reports the new advances in biological fractal dynamics. The following contents are included: (1) physical (or functional) fractal spaces abstracted from biological materials, biological structures and biological motions; (2) fractal operators on fractal spaces; (3) 1/2-order fractional dynamics controlled by fractal operators; and (4) the origin of 1/2-order. Based on the new progress, we can make a judgment that all the two-bifurcation physical functional fractal motions in the living body can be attributed to the fractional dynamics with 1/2-order.
Business Model Development for a High-Temperature (Co-)Electrolyser System
Authors: Riester Christian Michael , García Gotzon , Alayo Nerea , Tarancón Albert , Santos Diogo M. F. , Torrell Marc
Published: July 2022

There are increasing international efforts to tackle climate change by reducing the emission of greenhouse gases. As such, the use of electrolytic hydrogen as an energy carrier in decentralised and centralised energy systems, and as a secondary energy carrier for a variety of applications, is projected to grow. Required green hydrogen can be obtained via water electrolysis using the surplus of renewable energy during low electricity demand periods. Electrolysis systems with alkaline and polymer electrolyte membrane (PEM) technology are commercially available in different performance classes. The less mature solid oxide electrolysis cell (SOEC) promises higher efficiencies, as well as co-electrolysis and reversibility functions. This work uses a bottom-up approach to develop a viable business model for a SOEC-based venture. The broader electrolysis market is analysed first, including conventional and emerging market segments. A further opportunity analysis ranks these segments in terms of business attractiveness. Subsequently, the current state and structure of the global electrolyser industry are reviewed, and a ten-year outlook is provided. Key industry players are identified and profiled, after which the major industry and competitor trends are summarised. Based on the outcomes of the previous assessments, a favourable business case is generated and used to develop the business model proposal. The main findings suggest that grid services are the most attractive business sector, followed by refineries and power-to-liquid processes. SOEC technology is particularly promising due to its co-electrolysis capabilities within the methanol production process. Consequently, an “engineering firm and operator” business model for a power-to-methanol plant is considered the most viable option.
Association of APOE Serum Levels and APOE ε2, ε3, and ε4 Alleles with Optic Neuritis
Authors: Momkute Liucija , Vilkeviciute Alvita , Gedvilaite Greta , Dubinskaite Gabriele , Kriauciuniene Loresa , Liutkeviciene Rasa
Published: July 2022

Optical neuritis (ON), otherwise known as optical nerve damage, is a term used to describe various environmental and body conditions that lead to optic nerve dysfunction. Neurologists are well aware of conditions that cause optic neuropathy, such as trauma, infections, malnutrition, and various toxins. As optic neuritis is a multifactorial demyelinating or infectious process, genetic predisposition may also influence the progression of optic neuritis. This study aimed to evaluate the association of ON (with and without multiple sclerosis) with APOE alleles and APOE serum levels. We found that the APOE ε3/ε3 genotype was statistically less common in the ON group of males than in the control group (p = 0.045). Moreover, the APOE ε3/ε3 genotype had a 3.7-fold increase in the odds of ON development in males (OR = 3.698; CI: 1.503–9.095; p = 0.004). In contrast, the APOE ε3/ε4 genotype had a 4.1-fold decrease in the odds of ON development in males (OR = 0.242; CI: 0.083–0.704; p = 0.009). APOE serum levels were statistically significantly higher in the ON group than in the control group (p = 0.042). The APOE ε3/ε3 genotype may increase males’ risk of developing ON, while the ε3/ε4 genotype may reduce males’ risk of developing ON.
TTC30A and TTC30B Redundancy Protects IFT Complex B Integrity and Its Pivotal Role in Ciliogenesis
Authors: Hoffmann Felix , Bolz Sylvia , Junger Katrin , Klose Franziska , Schubert Timm , Woerz Franziska , Boldt Karsten , Ueffing Marius , Beyer Tina
Published: July 2022

Intraflagellar transport (IFT) is a microtubule-based system that supports the assembly and maintenance of cilia. The dysfunction of IFT leads to ciliopathies of variable severity. Two of the IFT-B components are the paralogue proteins TTC30A and TTC30B. To investigate whether these proteins constitute redundant functions, CRISPR/Cas9 was used to generate single TTC30A or B and double-knockout hTERT-RPE1 cells. Ciliogenesis assays showed the redundancy of both proteins while the polyglutamylation of cilia was affected in single knockouts. The localization of other IFT components was not affected by the depletion of a single paralogue. A loss of both proteins led to a severe ciliogenesis defect, resulting in no cilia formation, which was rescued by TTC30A or B. The redundancy can be explained by the highly similar interaction patterns of the paralogues; both equally interact with the IFT-B machinery. Our study demonstrates that a loss of one TTC30 paralogue can mostly be compensated by the other, thus preventing severe ciliary defects. However, cells assemble shorter cilia, which are potentially limited in their function, especially because of impaired polyglutamylation. A complete loss of both proteins leads to a deficit in IFT complex B integrity followed by disrupted IFT and subsequently no cilia formation.
Overview of Gene Special Issue “Genetic Conditions Affecting the Skeleton: Congenital, Idiopathic Scoliosis and Arthrogryposis”
Authors: Giampietro Philip F. , Hadley-Miller Nancy , Raggio Cathy L.
Published: July 2022

In this Special Issue of Genes entitled “Genetic Conditions Affecting the Skeleton: Congenital, Idiopathic Scoliosis and Arthrogryposis”, evidence is presented which suggests that congenital, idiopathic scoliosis, and arthrogryposis share similar overlapping, but also distinct etiopathogenic mechanisms, including connective tissue and neuromuscular mechanisms [...]
Transcriptional Interference Regulates the Evolutionary Development of Speech
Authors: Mortlock Douglas P. , Fang Zhi-Ming , Chandler Kelly J. , Hou Yue , Bickford Lissett R. , de Bock Charles E. , Eapen Valsamma , Clarke Raymond A.
Published: July 2022

The human capacity to speak is fundamental to our advanced intellectual, technological and social development. Yet so very little is known regarding the evolutionary genetics of speech or its relationship with the broader aspects of evolutionary development in primates. In this study, we describe a large family with evolutionary retrograde development of the larynx and wrist. The family presented with severe speech impairment and incremental retrograde elongations of the pisiform in the wrist that limited wrist rotation from 180° to 90° as in primitive primates. To our surprise, we found that a previously unknown primate-specific gene TOSPEAK had been disrupted in the family. TOSPEAK emerged de novo in an ancestor of extant primates across a 540 kb region of the genome with a pre-existing highly conserved long-range laryngeal enhancer for a neighbouring bone morphogenetic protein gene GDF6. We used transgenic mouse modelling to identify two additional GDF6 long-range enhancers within TOSPEAK that regulate GDF6 expression in the wrist. Disruption of TOSPEAK in the affected family blocked the transcription of TOSPEAK across the 3 GDF6 enhancers in association with a reduction in GDF6 expression and retrograde development of the larynx and wrist. Furthermore, we describe how TOSPEAK developed a human-specific promoter through the expansion of a penta-nucleotide direct repeat that first emerged de novo in the promoter of TOSPEAK in gibbon. This repeat subsequently expanded incrementally in higher hominids to form an overlapping series of Sp1/KLF transcription factor consensus binding sites in human that correlated with incremental increases in the promoter strength of TOSPEAK with human having the strongest promoter. Our research indicates a dual evolutionary role for the incremental increases in TOSPEAK transcriptional interference of GDF6 enhancers in the incremental evolutionary development of the wrist and larynx in hominids and the human capacity to speak and their retrogression with the reduction of TOSPEAK transcription in the affected family.
Effects of SNPs in AANAT and ASMT Genes on Milk and Peripheral Blood Melatonin Concentrations in Holstein Cows (Bos taurus)
Authors: Yao Songyang , Liu Yunjie , Liu Xuening , Liu Guoshi
Published: July 2022

Aralkylamine N-acetyltransferase (AANAT) and acetylserotonin O-methyltransferase (ASMT), the two rate-limiting enzymes for melatonin synthesis, regulate melatonin production in mammals. Through analysis of the milk melatonin level and dairy herd improvement (DHI) index, it was found that the melatonin concentration in milk was significantly negatively correlated with the 305 day milk yield (305M) and peak milk yield (PeakM) (p < 0.05), while it was significantly positively correlated with the serum melatonin concentration (p < 0.05). The full-length of AANAT and ASMT were sequenced and genotyped in 122 cows. Three SNPs in AANAT and four SNPs in ASMT were significantly related to MT levels in the milk and serum (p < 0.05). The SNPs in AANAT were temporarily denoted as N-SNP1 (g.55290169 T>C), N-SNP2 (g.55289357 T>C), and N-SNP3 (g.55289409 C>T). The SNPs in ASMT were temporarily denoted as M-SNP1 (g.158407305 G>A), M-SNP2 (g.158407477 A>G), M-SNP3 (g.158407874 G>A), and M-SNP4 (g.158415342 T>C). The M-SNP1, M-SNP2, and M-SNP3 conformed to the Hardy−Weinberg equilibrium (p > 0.05), while other SNPs deviated from the Hardy−Weinberg equilibrium (p < 0.05). The potential association of MT production and each SNP was statistically analyzed using the method of linkage disequilibrium (LD). The results showed that N-SNP2 and N-SNP3 had some degree of LD (D′ = 0.27), but M-SNP1 and M-SNP2 had a strong LD (D′ = 0.98). Thus, the DHI index could serve as a prediction of the milk MT level. The SNPs in AANAT and ASMT could be used as potential molecular markers for screening cows to produce high melatonin milk.
Immune-Related Genomic Schizophrenic Subtyping Identified in DLPFC Transcriptome
Authors: Childers Eva , Bowen Elijah F. W. , Rhodes C. Harker , Granger Richard
Published: July 2022

Well-documented evidence of the physiologic, genetic, and behavioral heterogeneity of schizophrenia suggests that diagnostic subtyping may clarify the underlying pathobiology of the disorder. Recent studies have demonstrated that increased inflammation may be a prominent feature of a subset of schizophrenics. However, these findings are inconsistent, possibly due to evaluating schizophrenics as a single group. In this study, we segregated schizophrenic patients into two groups (“Type 1”, “Type 2”) by their gene expression in the dorsolateral prefrontal cortex and explored biological differences between the subgroups. The study included post-mortem tissue samples that were sequenced in multiple, publicly available gene datasets using different sequencing methods. To evaluate the role of inflammation, the expression of genes in multiple components of neuroinflammation were examined: complement cascade activation, glial cell activation, pro-inflammatory mediator secretion, blood–brain barrier (BBB) breakdown, chemokine production and peripheral immune cell infiltration. The Type 2 schizophrenics showed widespread abnormal gene expression across all the neuroinflammation components that was not observed in Type 1 schizophrenics. Our results demonstrate the importance of separating schizophrenic patients into their molecularly defined subgroups and provide supporting evidence for the involvement of the immune-related pathways in a schizophrenic subset.
GALNT2 rs4846914 SNP Is Associated with Obesity, Atherogenic Lipid Traits, and ANGPTL3 Plasma Level
Authors: Qaddoumi Mohammad , Hebbar Prashantha , Abu-Farha Mohamed , Al Somaly Aseelah , Melhem Motasem , Al-Kayal Fadi , AlKhairi Irina , Cherian Preethi , Alanbaei Muath , Al-Mulla Fahd , Abubaker Jehad , Thanaraj Thangavel Alphonse
Published: July 2022

N-Acetylgalactosaminyltransferase 2 (GALNT2) is associated with serum lipid levels, insulin resistance, and adipogenesis. Additionally, angiopoietin-like (ANGPTL) proteins have emerged as regulators of lipoprotein lipase and lipid metabolism. In this study, we evaluated the association between GALNT2 rs4846914 variant, known for its association with lipid levels in European cohorts, with plasma levels of ANGPTL proteins, apolipoproteins, lipids, and obesity traits in individuals of Arab ethnicity. GALNT2 rs4846914 was genotyped in a cohort of 278 Arab individuals from Kuwait. Plasma levels of ANGPTL3 and ANGPTL8 were measured by ELISA and apolipoproteins by Luminex multiplexing assay. Allele-based association tests were performed with Bonferroni-corrected p-value thresholds. The GALNT2 rs4846914_G allele was associated with increased ANGPTL3 (p-values ≤ 0.05) but not with ANGPTL8 plasma levels. The allele was associated significantly with higher BMI and weight (p-values < 0.003), increased ApoC1 levels (p-values ≤ 0.006), and reduced HDL levels (p-values ≤ 0.05). Individuals carrying the GG genotype showed significantly decreased HDL and increased BMI, WC, ApoC1, and TG. Interactions exist between (AG+GG) genotypes and measures of percentage body fat, ApoA1A, ApoC1, and ApoB48-mediated HDL levels. GALNT2 is confirmed further as a potential link connecting lipid metabolism and obesity and has the potential to be a drug target for treating obesity and dyslipidemia.
Latent Class Analysis of Decompression Sickness Symptoms of Women Divers
Authors: Kim Da-Jung , Han Jeong-Won
Published: July 2022

This study aimed to investigate the types of clinical manifestations of decompression sickness among women divers (haenyeos) in Jeju using latent class analysis and to identify factors related to the condition. A total of 527 haenyeos who received their certification in diving fishery from Jeju and were working from 15 March to 31 May 2021 were included in this study. According to the results of the study, the latent classes were classified into type 1, type 2, and mixed symptoms groups (Akaike information criterion (AIC) = 6587.29, Bayesian information criterion (BIC) = 6698.23, sample size-adjusted BIC (saBIC) = 6615.70). For personal characteristics, age (χ2 = 40.31, p < 0.001) and education level (χ2 = 28.15, p < 0.001) showed a significant difference by latent class type. For work-related characteristics, diving experience (χ2 = 29.99, p < 0.001) and break time (χ2 = 9.32, p = 0.011) showed a significant difference by latent class type. The health-related characteristics, menopausal period (χ2 = 40.10, p < 0.001), body mass index (χ2 = 14.80, p = 0.013), and fatigue level (χ2 = 58.23, p < 0.001), showed a significant difference by latent class type. Rather than approaching the management of work-related diseases simply from the work environment perspective, it is important to increase the availability of health professionals who are capable of continuous health monitoring and management of women divers in their workplace.
Analysis of Factors Hindering the Dissemination of Medical Information Standards
Authors: Mukai Masami , Ogasawara Katsuhiko
Published: July 2022

Many medical information standards are not widely used in Japan, and this hinders the promotion of the use of real-world data. However, the complex intertwining of many factors hindering the dissemination of medical information standards makes it difficult to solve this problem. This study analyzed and visualized relationships among factors that inhibit the dissemination of medical information standards. Five medical informatics experts affiliated with universities and hospitals were interviewed about the factors that hinder the dissemination of medical information standards in Japan. The presented factors were analyzed using the interpretive structural modeling (ISM) method and the decision-making trial and evaluation laboratory (DEMATEL) method. We found that “legislation” and “reliability” were important inhibiting factors for the dissemination of medical information standards in Japan. We also found a six-layered structure in which “reliability” was satisfied when “legislation” was in place and “expectations” and “personal information” were resolved. The DEMATEL analysis indicated the relationships and classifications of factors hindering the dissemination of medical information standards. Since the adoption of medical information standards does not directly lead to revenue for medical institutions, it is possible to meet the “expectation” of improving the quality of medical care by ensuring “legislation” and “reliability”, that is, ensuring the dependability of medical treatment. The results of this study visually show the structure of the factors and will help solve the problems that hinder the effective and efficient spread of standards. Solving these problems may support the efficient use of real-world data.
Prognosis of Implants with Implant-Supported Fixed Dental Prostheses in the Elderly Population: A Retrospective Study with a 5- to 10-Year Follow-Up
Authors: Takahashi Tomoyo , Kihara Masafumi , Oki Kyosuke , Matsuzaki Tatsuya , Ayukawa Yasunori , Matsushita Yasuyuki , Koyano Kiyoshi
Published: July 2022

This retrospective study aimed to investigate the survival rate of implants from 5 to 10 years after the placement of implant-supported fixed dental prostheses (ISFDPs) and the management of implant loss in the elderly population. Elderly patients (≥65 years old) who had been treated with ISFDPs and followed up with for at least 5 years between October 2009 and March 2020 were enrolled. Patient profiles and implant-related data were extracted. The survival rate of implants up to 5 years as well as the 10-year cumulative survival rate were evaluated. The management of implant loss and prosthetic interventions were also investigated. In total, 195 patients (mean age: 70.1 ± 4.5 years old) and 687 implants (287 ISFDPs) were assessed. The 5-year survival rate was 99.0% and the 10-year cumulative survival rate was 98.1%. Seven of the eleven implants lost were lost due to peri-implantitis. Only three implants in two patients were placed after the loss of the implants; most were restored using non-invasive procedures. Two patients underwent a conversion from ISFDPs to removable prostheses. This study showed that high survival rates were observed in an elderly population with ISFDPs and that non-invasive procedures were often applied after the loss of an implant.