bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of foodborne viruses and influenza A virus from poultry processing plants to retailed chickens
Authors: Yeo Daseul , Song Mengxiao , Hossain Md. Iqbal , Jung Soontag , Wang Zhaoqi , Seo Dong Joo , Rhee Min Suk , Choi Changsun
Published: March 2023

Introduction

Foodborne viruses are a serious concern in public health. This study investigated the prevalence of eight foodborne viruses norovirus (NoV), adenovirus (AdV), sapovirus (SapoV), astrovirus, hepatitis A virus (HAV), hepatitis E virus (HEV), rotavirus, aichivirus, and influenza A virus (IAV).

Material and method

A total of 316 chicken samples were collected from three poultry processing plants to commercial markets (local and online). RT-qPCR- and PCR-positive amplicons obtained from monitoring were confirmed by sequence analysis.

Results

Foodborne viruses and IAV were not found in poultry processing plants. Of the 100 chickens purchased from the local and online markets, 19 (19.0%) AdV and 2 (2.0%) SapoV were detected. NoV, astrovirus, HAV, HEV, rotavirus, aichivirus, and IAV were not detected in the retailed chickens. Phylogenetic analysis identified 18 human AdV-41, one porcine AdV, and two SapoV-GI.1. It was the first case of the discovery of the SapoV gene in chicken. The average contamination level of detected AdV was 2.4 log DNA copies/g, but there were cases where the highest level was 5.35 log DNA copies/g.

Discussion

This study highlights the importance of chicken's contribution to the transmission of AdV with the possibility of annual variability with emerging symptoms. The prevention of AdV contamination in the food chain from slaughterhouses to retail markets should be monitored and controlled in further study.
Surgical treatment of patellar dislocation: A network meta-analysis of randomized control trials and cohort studies
Authors: Fang Mingqing , Cai Zijun , Pan Linyuan , Ding Yilan , Zhang Yueyao , Cheng Siyuan , Wang Yifan , Gao Jialin , Li Yusheng , Xiao Wenfeng
Published: March 2023

Background

Currently, there are many surgical options for patellar dislocation. The purpose of this study is to perform a network meta-analysis of the randomized controlled trials (RCTs) and cohort studies to determine the better treatment.

Method

We searched the Pubmed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, clinicaltrials.gov and who.int/trialsearch. Clinical outcomes included Kujala score, Lysholm score, International Knee Documentation Committee (IKDC) score, redislocation or recurrent instability. We conducted pairwise meta-analysis and network meta-analysis respectively using the frequentist model to compare the clinical outcomes.

Results

There were 10 RCTs and 2 cohort studies with a total of 774 patients included in our study. In network meta-analysis, double-bundle medial patellofemoral ligament reconstruction (DB-MPFLR) achieved good results on functional scores. According to the surface under the cumulative ranking (SUCRA), DB-MPFLR had the highest probabilities of their protective effects on outcomes of Kujala score (SUCRA 96.5 %), IKDC score (SUCRA 100.0%) and redislocation (SUCRA 67.8%). However, DB-MPFLR (SUCRA 84.6%) comes second to SB-MPFLR (SUCRA 90.4%) in Lyshlom score. It is (SUCRA 70%) also inferior to vastus medialis plasty (VM-plasty) (SUCRA 81.9%) in preventing Recurrent instability. The results of subgroup analysis were similar.

Conclusion

Our study demonstrated that MPFLR showed better functional scores than other surgical options.
Comprehensive comparison of three techniques for the treatment of adjacent segment degeneration after lumbar fusion
Authors: Li Tao , He Hang , Zhang Tonghui , Li Xugui , Xie Wei , Huang Biwang , Xu Feng , Xiong Chengjie
Published: March 2023

Purpose

Adjacent segment degeneration (ASD) following lumbar fusion is technically challenging for spine surgeons. Posterolateral open fusion surgery with pedicle screw fixation is an effective way to treat symptomatic ASD with favorable clinical outcomes; however, it is associated with an increased morbidity rate. Therefore, minimally invasive spine surgery is advocated. This study was designed to compare clinical outcomes among patients with symptomatic ASD who underwent percutaneous transforaminal endoscopic discectomy (PTED) with the transforaminal approach, posterior lumbar interbody fusion (PLIF) with cortical bone trajectory screw fixation (CBT-PLIF), and PLIF with traditional trajectory screw fixation (TT-PLIF).

Methods

A retrospective study was conductedon 46 patients (26 men and 20 women; average age 60.8 ± 6.78 years) with symptomatic ASD. The patients were treated with three approaches. The operation time, incision length, time to return to work, complications, and the like were compared among three groups. Intervertebral disc (IVD) space height, angular motion, and vertebral slippage were obtained to assess spine biomechanical stability following surgery. The visual analog scale (VAS) score and Oswestry disability index were evaluated at preoperation and 1-week, 3-month, and the latest follow-ups. Clinical global outcomes were also estimated using modified MacNab criteria.

Results

The operation time, incision length, intraoperative blood loss, and time to return to work for the PTED group were significantly decreased compared with those for the other two groups (P < 0.05). The radiological indicators in the CBT-PLIF group and TT-PLIF group had better biomechanical stability compared with those in the PTED groups at the latest follow-up (P < 0.05). The back pain VAS score in the CBT-PLIF group was significantly decreased compared with those in the other two groups at the latest follow-up (P < 0.05). The good-to-excellent rate was 82.35% in the PTED group, 88.89% in the CBT-PLIF group, and 85.00% in the TT-PLIF group. No serious complications were encountered. Two patients experienced dysesthesia in the PTED group; screw malposition was found in one patient in the CBT-PLIF group. One case with a dural matter tear was observed in the TT-PLIF group.

Conclusion

All three approaches can treat patients with symptomatic ASD efficiently and safely. Functional recovery was more accelerated in the PTED group compared with the other approaches in the short term; CBT-PLIF and TT-PLIF can provide superior biomechanical stability to the lumbosacral spine following decompression compared with PTED; however, compared with TT-PLIF, CBT-PLIF can significantly reduce back pain caused by iatrogenic muscle injury and improve functional recovery. Therefore, superior clinical outcomes were achieved in the CBT-PLIF group compared with the PTED and TT-PLIF groups in the long term.
Simultaneous arthroscopic cystectomy and unicompartmental knee arthroplasty for the management of partial knee osteoarthritis with a popliteal cyst: A case report
Authors: Liu Cai , Zhou Dejie , Liu Xinwei , Huang Jin , Fang Jianguo , Zhou Hongyu , Luo Jianjun , Luo Yiqian , Zhao Lianghu
Published: March 2023

Introduction

Popliteal cysts are secondary to degenerative changes in the knee joint. After total knee arthroplasty (TKA), 56.7% of patients with popliteal cysts at 4.9 years follow-up remained symptomatic in the popliteal area. However, the result of simultaneous arthroscopic cystectomy and unicompartmental knee arthroplasty (UKA) was uncertain.

Case presentation

A 57-year-old man was admitted to our hospital with severe pain and swelling in his left knee and the popliteal area. He was diagnosed with severe medial unicompartmental knee osteoarthritis (KOA) with a symptomatic popliteal cyst. Subsequently, arthroscopic cystectomy and unicompartmental knee arthroplasty (UKA) were performed simultaneously. A month after the operation, he returned to his normal life. There was no progression in the lateral compartment of the left knee and no recurrence of the popliteal cyst at the 1-year follow-up.

Conclusion

For KOA patients with a popliteal cyst seeking UKA, simultaneous arthroscopic cystectomy and UKA are feasible with great success if managed appropriately.
Risk factors for deep vein thrombosis in patients with pelvic or lower-extremity fractures in the emergency intensive care unit
Authors: Shi Dongcheng , Bao Bingbo , Zheng Xianyou , Wei Haifeng , Zhu Tianhao , Zhang Yi , Zhao Gang
Published: March 2023

Introduction

This study aimed to investigate the incidence of deep vein thrombosis (DVT) in patients with pelvic or lower-extremity fractures in the emergency intensive care unit (EICU), explore the independent risk factors for DVT, and investigate the predictive value of the Autar scale for DVT in these patients.

Methods

The clinical data of patients with single fractures of the pelvis, femur, or tibia in the EICU from August 2016 to August 2019 were retrospectively examined. The incidence of DVT was statistically analyzed. Logistic regression was used to analyze the independent risk factors for DVT in these patients. The receiver-operating characteristic (ROC) curve was used to evaluate the predictive value of the Autar scale for the risk of DVT.

Results

A total of 817 patients were enrolled in this study; of these, 142 (17.38%) had DVT. Significant differences were found in the incidence of DVT among the pelvic fractures, femoral fractures, and tibial fractures (P < 0.001). The multivariate logistic regression analysis showed multiple injuries (OR = 2.210, 95% CI: 1.166–4.187, P = 0.015), fracture site (compared with tibia fracture group, femur fracture group OR = 4.839, 95% CI: 2.688–8.711, P < 0.001; pelvic fracture group OR = 2.210, 95% CI: 1.225–3.988, P = 0.008), and Autar score (OR = 1.198, 95% CI: 1.016–1.353, P = 0.004) were independent risk factors for DVT in patients with pelvic or lower-extremity fractures in the EICU. The area under the ROC curve (AUROC) of the Autar score for predicting DVT was 0.606. When the Autar score was set as the cutoff value of 15.5, the sensitivity and specificity for predicting DVT in patients with pelvic or lower-extremity fractures were 45.1% and 70.7%, respectively.

Discussion

Fracture is a high-risk factor for DVT. Patients with a femoral fracture or multiple injuries have a higher risk of DVT. In the case of no contraindications, DVT prevention measures should be taken for patients with pelvic or lower-extremity fractures. Autar scale has a certain predictive value for the occurrence of DVT in patients with pelvic or lower-extremity fractures, but it is not ideal.
Monitoring of antimicrobial usage among adult bovines in dairy herds of Punjab, India: A quantitative analysis of pattern and frequency
Authors: Vijay Deepthi , Bedi Jasbir Singh , Dhaka Pankaj , Singh Randhir , Singh Jaswinder , Arora Anil Kumar , Gill Jatinder Paul Singh
Published: March 2023

The present study aimed to evaluate the antimicrobial usage (AMU) pattern in dairy herds of Punjab, India. The on-farm quantification of AMU in adult bovine animals by the manual collection of empty drug containers (“bin method”) along with the records of the treatment was carried out in 38 dairy farms involving 1010 adult bovines for 1 year from July 2020 to June 2021. The farm owners were asked to record the antibiotic treatments as well as to deposit empty antibiotic packaging/vials into the provided bins placed at the farms. A total of 14 different antibiotic agents in 265 commercial antibiotic products were administered to the dairy herds during the study. A total of 179 (67.55%) administered products contained antimicrobials of “critical importance” as per the World Health Organization (WHO). Mastitis (54.72%), followed by the treatment of fever (19.62%), reproductive problems (15.47%), and diarrhea (3.40%) accounted for the majority of drugs administered in the herds during the study period. The most commonly used antibiotics were enrofloxacin (89.47% herds; 21.51% products), followed by ceftriaxone (50% herds; 12.83% products), amoxicillin (50% herds; 12.83% products), oxytetracycline (55.26% herds; 11.70% products), and procaine penicillin (47.37% herds; 12.83% products). The highest quantity of AMU [in terms of antimicrobial drug use rate (ADUR)] was observed for ceftiofur, followed by ceftriaxone, procaine benzyl penicillin ceftizoxime, enrofloxacin, cefoperazone, amoxicillin and ampicillin. A total of 125 (47.17%) products contained “highest priority critically important antimicrobials” (HPCIA) and 54 (20.37%) products contained “high priority critically important antimicrobials”. In terms of overall number of animal daily doses (nADD), the highest priority critically important antimicrobials (HPCIA) of the WHO such as third-generation cephalosporins and quinolones, respectively accounted for 44.64 and 22.35% of the total antibiotic use in the herds. The bin method offers an alternative to monitoring AMU as a more accessible approach for recording the actual consumption of antimicrobials. The present study, to the best of our knowledge, is the first of its kind to provide an overview of the qualitative and quantitative estimate of AMU among adult bovines from India.
Dietary supplementation with a complex of cinnamaldehyde, carvacrol, and thymol negatively affects the intestinal function in LPS-challenged piglets
Authors: Zhang Yanyan , Li Qian , Wang Zhongxing , Dong Yi , Yi Dan , Wu Tao , Wang Lei , Zhao Di , Hou Yongqing
Published: March 2023

Background

The effects of cinnamaldehyde, carvacrol and thymol complex (CCT) on the growth performance and intestinal function of piglets challenged with lipopolysaccharide (LPS) were determined. Colistin sulphate (CS) was as a positive control.

Method

Piglets (n = 24, 32 days of age) were allocated to four treatments: Control group (fed basal diet), LPS group (fed basal diet), CS+LPS group (fed basal diet + 50 mg/kg CS), and CCT+LPS group (fed basal diet + 50 mg/kg CCT).

Results

Results showed that diarrhea rates of piglets were significantly reduced by CCT and CS supplementation respectively. Further research showed that CS supplementation tended to improve the intestinal absorption function in LPS-challenged piglets. Moreover, CS supplementation significantly reduced the contents of cortisol in blood and malondialdehyde in the duodenum and the activities of inducible nitric oxide synthase in the duodenum and ileum and total nitric oxide synthase in the ileum in LPS-challenged piglets. CS supplementation significantly increased the activities of sucrase in the ileum and myeloperoxidase in the jejunum in LPS-challenged piglets. CS supplementation significantly alleviated the reduced mRNA levels of immune-related genes (IL-4, IL-6, IL-8, IL-10) in mesenteric lymph nodes and jejunum and mucosal growth-related genes (IGF-1, mTOR, ALP) in LPS-challenged piglets. These results suggested that CS supplementation improved the intestinal function in LPS-challenged piglets by improving intestinal oxidative stress, immune stress, and absorption and repair function. However, although CCT supplementation improved oxidative stress by reducing (p < 0.05) the content of malondialdehyde and the activity of nitric oxide synthase in the duodenum, CCT supplementation tended to aggravate the intestinal absorption dysfunction in LPS-challenged piglets. Furthermore, compared with the control and LPS groups, CCT supplementation remarkably elevated the content of prostaglandin in plasma and the mRNA levels of pro-inflammatory factor IL-6 in mesenteric lymph nodes and jejunum, and reduced the activity of maltase in the ileum in LPS-challenged piglets. These results suggested that CCT supplementation had a negative effect on intestinal function by altering intestinal immune stress response and reducing disaccharidase activity in LPS-challenged piglets.

Conclusions

Compared to CS, CCT supplementation exhibited a negative effect on intestinal function, suggesting whether CCT can be as an effective feed additive still needs further study.
Antibacterial activity and mechanism of sanguinarine against Staphylococcus aureus by interfering with the permeability of the cell wall and membrane and inducing bacterial ROS production
Authors: Gu Yeqing , Dong Jun , Li Jing , Luo Qianmin , Dong Xianlan , Tang Guowen , Zhang Jiaxiang , Du Xuan , Pu Qiqi , He Lin , Zhao Kaiwei , Han Diangang , Xin Jige
Published: March 2023

Staphylococcus aureus (SA) is representative of gram-positive bacteria. Sanguinarine chloride hydrate (SGCH) is the hydrochloride form of sanguinarine (SG), one of the main extracts of Macleaya cordata (M. cordata). There are few reports on its antibacterial mechanism against SA. Therefore, in this study, we investigated the in vitro antibacterial activity and mechanism of SGCH against SA. The inhibitory zone, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were measured, and the bactericidal activity curve was plotted. In addition, the micromorphology, alkaline phosphatase (AKP) activity, Na+K+, Ca2+Mg2+-adenosine triphosphate (ATP) activity, intracellular reactive oxygen species (ROS), and fluorescein diacetate (FDA) were observed and detected. The results showed that the inhibitory zone of SGCH against SA was judged as medium-sensitive; the MIC and MBC were 128 and 256 μg/mL, respectively; in the bactericidal activity curve, SGCH with 8 × MIC could completely kill SA within 24 h. SGCH was able to interfere with the integrity and permeability of the SA cell wall and membrane, as confirmed by the scanning electron microscopy (SEM) images, the increase in extracellular AKP and Na+ K+, Ca2+ Mg2+-ATP activities as well as the fluorescein diacetate (FDA) staining experiment results. Moreover, a high concentration of SGCH could induce SA to produce large amounts of ROS. In summary, these findings revealed that SGCH has a preferable antibacterial effect on SA, providing an experimental and theoretical basis for using SG as an antibiotic substitute in animal husbandry and for the clinical control and treatment of diseases caused by SA.
Establishment and characterization of a multi-drug resistant cell line for canine mammary tumors
Authors: Zhou Chaoyu , Lin Zixiang , Li Xinqiu , Zhang Di , Song Peijia
Published: March 2023

Background and purpose

Canine mammary tumors are the most common tumor disease of female dogs, and adjuvant chemotherapy often results in multi-drug resistance. Currently, the mechanisms underlying the development of tumor multi-drug resistance are unclear. The translation of research applications that can be used to effectively overcome tumor resistance is similarly hampered. Therefore, it is urgent to construct multi-drug resistance models of canine mammary tumors that can be used for research, to explore the mechanisms and means of overcoming resistance.

Materials and methods

In this study, the canine triple negative breast cancer cell line CMT-7364 was induced to develop multidrug resistance using doxorubicin by high-dose drug pulse method. The drug resistance and the expression of drug transport pumps of the cells was verified by CCK8 assay, immunoblotting, qPCR and immunofluorescence. Next, we used scratch assay and Transwell invasion assay to compare the migration and invasion abilities of the two cell lines and examined the expression of EMT-related proteins in both using immunoblotting. The differences of transcriptome between parental and drug-resistant cell lines were detected by RNA-seq sequencing. Finally, mouse xenograft models of drug-resistant and parental cell lines were constructed to evaluate the tumorigenic ability.

Results

After more than 50 generations of continuous passages stimulated by high-dose drug pulse method, the morphology of drug-resistant cell line CMT-7364/R tended to be mesenchymal-like and heterogeneous under light microscopy compared with the parental cell line CMT-7364/S, and developed resistance to doxorubicin and other commonly used chemotherapeutic drugs. In CMT-7364/R, BCRP was expressed at higher levels at both transcriptional and protein levels, while P-glycoprotein was not significantly different. Secondly, the migration and invasion ability of CMT-7364/R was significantly enhanced, with decreased expression of E-cadherin and increased expression of vimentin and mucin 1-N terminus. Finally, mouse xenograft models were constructed, while there was no significant difference in the volume of masses formed at 21 days.

Conclusion

In summary, by using the canine mammary tumor cell line CMT-7364/S as the parental cell line, we successfully constructed a multidrug-resistant CMT-7364/R with high-dose drug pulse methods. Compared to its parental cell line, CMT-7364/R has decreased growth rate, overexpression of BCRP and increased migration and invasion ability due to EMT. The results of this study showed that CMT-7364/R might serve as a model for future studies on tumor drug resistance.
The inhibition of high ammonia to in vitro rumen fermentation is pH dependent
Authors: Shen Junshi , Zheng Wenjin , Xu Yixuan , Yu Zhongtang
Published: March 2023

Ammonia is an important rumen internal environment indicator. In livestock production, feeding a large amount of non-protein nitrogen to ruminants will create high ammonia stress to the animals, which increases the risk of ammonia toxicity. However, the effects of ammonia toxicity on rumen microbiota and fermentation are still unknown. In this study, an in vitro rumen fermentation technique was used to investigate the effects of different concentrations of ammonia on rumen microbiota and fermentation. To achieve the four final total ammonia nitrogen (TAN) concentrations of 0, 8, 32, and 128 mmol/L, ammonium chloride (NH4Cl) was added at 0, 42.8, 171.2, and 686.8 mg/100 mL, and urea was added at 0, 24, 96, and 384 mg/100 mL. Urea hydrolysis increased, while NH4Cl dissociation slightly reduced the pH. At similar concentrations of TAN, the increased pH of the rumen culture by urea addition resulted in a much higher free ammonia nitrogen (FAN) concentration compared to NH4Cl addition. Pearson correlation analysis revealed a strong negative correlation between FAN and microbial populations (total bacteria, protozoa, fungi, and methanogens) and in vitro rumen fermentation profiles (gas production, dry matter digestibility, total volatile fatty acid, acetate, propionate, etc.), and a much weaker correlation between TAN and the above indicators. Additionally, bacterial community structure changed differently in response to TAN concentrations. High TAN increased Gram-positive Firmicutes and Actinobacteria but reduced Gram-negative Fibrobacteres and Spirochaetes. The current study demonstrated that the inhibition of in vitro rumen fermentation by high ammonia was pH-dependent and was associated with variations of rumen microbial populations and communities.
Evaluation of a Soft Sensor Concept for Indirect Flow Rate Estimation in Solenoid-Operated Spool Valves
Authors: Hucko Simon , Krampe Hendrik , Schmitz Katharina
Published: March 2023

Concepts, such as power and condition monitoring or smart systems, are becoming increasingly important but require extensive insight into the process or machine, which is mostly gained by additional sensors. However, this development is contrasted by growing global competition and cost pressure. The measurement concept of a soft flow rate sensor presented here, addresses this discrepancy by means of a cost-effective software-based sensor, utilizing the dependence between flow rate and flow force in hydraulic spool valves. In the presented work, the feasibility of the introduced approach and the anticipated challenges, such as the modeling of disturbances in the mechanical, fluidic and electromagnetic subdomain, are assessed. For this purpose, important phenomena influencing measurement accuracy are identified on the basis of previous work. With the help of these findings, a greatly simplified version of the soft sensor is built and evaluated with a commercial valve to perform an initial test of the concept. Regardless of future implementations, the soft sensor concept presented here may have limitations in terms of dynamics and accuracy. However, the aim is not to replace a classic flow rate sensor, such as a gear sensor, but rather to create a cost-effective way to determine the flow rate without any additional integration effort.
Defining the Climate for Inclusiveness and Multiculturalism: Linking to Context
Authors: Cunningham John Barton
Published: March 2023

The purpose of this paper is to develop a better understanding of how to define a positive climate for inclusiveness that recognizes the context and social environment of participants. In order to study employees working with Indigenous people and minorities in four organizations, we used a grounded research approach to define what an inclusive environment might look like. The interview questions gathered examples of experiences which employees valued because they felt more included and not excluded from people they worked with. The experiences fell into four categories, as follows: (i) leadership engaged in supporting inclusiveness within the organization; (ii) leadership engaged in seeking inclusiveness within the community; (iii) being involved in multicultural practices within the organization and community; and (iv) participating in initiatives which encourage engagement and involvement. This paper’s conceptualization of a climate of inclusion is different from other studies, possibly because of the unique context in which service organizations are placed, as such organizations typically work with Indigenous people and minorities. Although we are especially mindful of the danger of generalizing our findings without further research, the scope of this paper might provide some direction for future studies of other organizations. We suggest that there is also a need to be open to methods which allow individuals and groups to define a climate of inclusivity that is relevant to their context; this is because context may be essential for recognizing certain groups of people.
Effect of Working Current on C12A7 Hollow Cathode
Authors: Huang Yajun , Wang Xiaoxian , Cui Guomin , Wang Pingyang , Cai Dongsheng
Published: March 2023

The C12A7 hollow cathode is expected to usher in a new generation of hollow cathodes because of its low work function and chemical stability. In order to reduce the emission melting and degradation caused by the overheating of this new cathode, different working currents of the C12A7 hollow cathode were studied in this work. The working currents ranged from 1 A to 20 A. The results show that the C12A7 hollow cathode works well under the condition of a low current, but it is unstable under high current condition. A simulation with the COMSOL internal working environment showed that enlarging the cathode orifice size is beneficial to reduce the risk of overheating of the electride emitter. The C12A7 hollow cathode shows a voltage transition phenomenon under a high operating current. The plasma plume state at the cathode outlet gradually changes from bright violet to dark yellow. The emitter also melts at high temperatures under high-current conditions. Its short lifetime makes the C12A7 hollow cathode difficult to apply in practical application scenarios under high-current conditions.
A Shortlisting Framework for Crop Diversification in the United Kingdom
Authors: Jahanshiri Ebrahim , Azam-Ali Sayed , Gregory Peter J. , Wimalasiri Eranga M.
Published: March 2023

We present a systematic framework for nationwide crop suitability assessment within the UK to improve the resilience in cropping systems and nutrition security of the UK population. An initial suitability analysis was performed using data from 1842 crops at 2862 grid locations within the UK, using climate (temperature and rainfall) and soil (pH, depth, and texture) data from the UK Met Office and British Geological Survey. In the second phase, additional qualitative and quantitative data are collected on 56 crops with the highest pedoclimatic suitability and coverage across the UK. An exercise was conducted on crops within each category using a systematic ranking methodology that shortlists crops with high value across a multitude of traits. Crops were ranked based on their nutritional value (macronutrients, vitamins, and minerals) and on adaptive (resistance to waterlogging/flood, frost, shade, pest, weed, and diseases and suitability in poor soils) and physiological traits (water-use efficiency and yield). Other characteristics such as the number of special uses, available germplasm through the number of institutions working on the crops, and production knowledge were considered in shortlisting. The shortlisted crops in each category are bulbous barley (cereal), colonial bentgrass (fodder), Russian wildrye (forage), sea buckthorn (fruit), blue lupin (legume), shoestring acacia (nut), ochrus vetch (vegetable), spear wattle (industrial), scallion (medicinal), and velvet bentgrass (ornamental/landscape). These crops were identified as suitable crops that can be adopted in the UK. We further discuss steps in mainstreaming these and other potential crops based on a systematic framework that takes into account local farming system issues, land suitability, and crop performance modelling at the field scale across the UK.
An Application of Artificial Neural Network for Predicting Threshing Performance in a Flexible Threshing Device
Authors: Ma Lan , Xie Fangping , Liu Dawei , Wang Xiushan , Zhang Zhanfeng
Published: March 2023

Rice is a widely cultivated food crop worldwide, and threshing is one of the most important operations of combine harvesters in grain production. It is a complex, nonlinear, multi-parameter physical process. The flexible threshing device has unique advantages in reducing the grain damage rate and has already been one of the major concerns in engineering design. Using the measured test database of the flexible threshing test bench, the rotation speed of the threshing cylinder (RS), threshing clearance of the concave sieve (TC), separation clearance of the concave sieve (SC), and feeding quantity (FQ) are used as the input layer. In contrast, the crushing rate (YP), impurity rate of the threshed material (YZ), and loss rate (YS) are used in the output layer. A 4-5-3-3 artificial neural network (ANN) model, with a backpropagation learning algorithm, was developed to predict the threshing performance of the flexible threshing device. Next, we explored the degree to which the inputs affect the outputs. The results showed that the R of the threshing performance model validation set in the hidden layer reached 0.980, and the root mean square error (RMSE) and the average absolute error (MAE) were less than 0.139 and 0.153, respectively. The built neural network model predicted the performance of the flexible threshing device, and the regression determination coefficient R2 between the prediction data and the experimental data was 0.953. The results showed revealed that the data combined with the ANN method is an effective approach for predicting the threshing performance of the flexible threshing device in rice. Moreover, the sensitivity analysis showed that RS, TC, and SC were crucial factors influencing the performance of the flexible threshing device, with an average relative importance of 15.00%, 14.89%, and 14.32%, respectively. FQ had the least effect on threshing performance, with an average threshing relative importance of 11.65%. Our findings can be leveraged to optimize the threshing performance of future flexible threshing devices.
Effects of Dietary Intervention Using Spirulina at Graded Levels on Productive Performance and Physiological Status of Quail Birds Reared under Elevated Temperatures
Authors: Nassar Farid S. , Alaqil Abdulaziz A. , El-Sayed Dalia A. A. , Kamel Nancy N. , Abbas Ahmed O.
Published: March 2023

The current study aimed to explore the effect of Spirulina platensis (SP) inclusion at various levels in quail diets, in terms of their production performance, physiological traits, stress measurements, and immunological parameters under heat stress (HS) conditions. Four hundred Japanese quail (Coturnix japonica) chicks, one day old, were equally distributed into forty wire cages, and the cages were placed in two chambers with environmentally controlled systems (20 cages in each chamber). From 21 to 42 d of age, the quails were randomly subjected to a factorial design of two HS treatments × four SP treatments. To induce HS treatments in the quails, the first chamber was maintained at a thermoneutral temperature of 24 °C (TN group), while the temperature of the second chamber was elevated to 35 °C during the daytime (9:00–17:00 h), followed by a thermoneutral temperature for the remaining 24 h cycle (HS group). The birds in each chamber were further allocated into four SP treatments (5 replicate cages × 10 birds per cage in each treatment), where the quails were fed on a basal diet that included 0, 5, 10, or 15 g/kg SP (SP0, SP5, SP10, and SP15 groups, respectively). After exposure to the HS, a significant (p < 0.05) reduction of 5% in body weight and 9% in both weight gain and feed intake was recorded, and the slaughter performance of the quails was adversely (p < 0.05) affected. In addition, HS significantly (p < 0.05) impaired the physiological traits (total protein, albumin, globulin, alanine transferase, aspartate transferase, creatinine, uric acid, cholesterol, and triglycerides) and immunological parameters (total white blood cells, heterophil to lymphocyte ratio, and T- and B-lymphocyte stimulation indexes), but increased the stress measurements (corticosterone, malondialdehyde, interleukin-1β, and tumor necrosis factor-α). In contrast, most of these parameters were linearly (p < 0.05) improved by increasing the SP levels in the diets of the TN quail group. When the SP was included in the diets of the HS quail group, the deleterious effects of HS on the alanine and aspartate transferase activities, creatinine, uric acid, triglycerides, corticosterone, interleukin-1β, and tumor necrosis factor-α levels, heterophil to lymphocyte ratio, and T- and B-lymphocyte stimulation indexes were remarkably (p < 0.05) relieved. These results concluded that SP nutritional application can improve the production performance and the overall physiological homeostasis of the Japanese quail, especially when suffering from heat stress.
Design and Application of Agricultural Equipment in Tillage Systems
Authors: Ucgul Mustafa , Chang Chung-Liang
Published: March 2023

Due to the rapid increase in world population, the demand for food has increased dramatically [...]
Estimating Body Weight in Captive Rabbits Based on Improved Mask RCNN
Authors: Duan Enze , Hao Hongyun , Zhao Shida , Wang Hongying , Bai Zongchun
Published: March 2023

Automated body weight (BW) estimation is an important indicator to reflect the automation level of breeding, which can effectively reduce the damage to animals in the breeding process. In order to manage meat rabbits accurately, reduce the frequency of manual intervention, and improve the intelligent of meat rabbit breeding, this study constructed a meat rabbit weight estimation system to replace manual weighing. The system consists of a meat rabbit image acquisition robot and a weight estimation model. The robot stops at each cage in turn and takes a top view of the rabbit through an RGB camera. The images from the robot are automatically processed in the weight estimation model, which consists of the meat rabbit segmentation network based on improved Mask RCNN and the BW fitting network. Attention mechanism, PointRend algorithm, and improved activation function are proposed to improve the performance of Mask RCNN. Six morphological parameters (relative projected area, contour perimeter, body length, body width, skeleton length, and curvature) are extracted from the obtained mask, and are sent into the BW fitting network based on SVR-SSA-BPNN. The experiment shows that the system achieves a 4.3% relative error and 172.7 g average absolute error in BW estimation for 441 rabbits, while the meat rabbit segmentation network achieves a 99.1% mean average precision (mAP) and a 98.7% mean pixel accuracy (MPA). The system provides technical support for automatic BW estimation of meat rabbits in commercial breeding, which is helpful to promote precision breeding.
The Effect of Farming Management and Crop Rotation Systems on Chlorophyll Content, Dry Matter Translocation, and Grain Quantity and Quality of Wheat (Triticum aestivum L.) Grown in a Semi-Arid Region of Iran
Authors: Gorooei Aram , Gaiser Thomas , Aynehband Amir , Rahnama Afrasyab , Kamali Bahareh
Published: March 2023

To find suitable farming management approaches in the semi-arid climate of Iran, we set up an experiment combining three farm management practices with four crop rotation systems over four growing seasons (two winter and two summer seasons), from 2018 to 2020. The three farm management practices comprised: intensive (IF, with inorganic inputs, removal of crop residues from the soil, and weeds chemically controlled), organic (OF, with organic inputs, a return 30% of crop residues in the soil, and weeds mechanically controlled), and integrated (INT, with mineral/organic inputs, return 15% of crop residues to the soil, integrated weed control). The four crop rotation systems were: fallow-wheat (F-W), maize-wheat (M-W), sesame-wheat (S-W), and mung bean-wheat (B-W). Treatment effects were assessed by chlorophyll (Chl) content, photosynthetic parameters, and wheat grain quality and quantity measurements. All management practices from the first to the second year resulted in increases in the total Chl content and post-anthesis photosynthesis (PAP). The total Chl content under INT was improved through a greater increase in Chl-b compared to Chl-a. Dry matter remobilisation (DMR) was higher under INT than under IF. The highest (39) and lowest (23) grain number per spike were obtained in IF under B-W and OF under F-W, respectively. B-W produced the highest grain yield (541.4 g m−2). The protein contents in farming with organic matter inputs were higher than that under IF. INT produced an optimum level of wheat yield despite a 50% reduction in chemical inputs, and this was achieved through the fast absorption of chemical elements at the beginning of growth, and having access—at the grain filling stage—to elements derived from organic matter decomposition, and through the utilisation of DMR. Our results indicate that implementing B-W and S-W under INT is a promising strategy for this region. However, the results need to be further evaluated by long-term experiments.
Cadmium Accumulation and Immobilization by Artemisia selengensis under Different Compound Amendments in Cadmium-Contaminated Soil
Authors: Wang Huiyan , Gao Zhou , Li Xun , Duan Zengqiang
Published: March 2023

Cadmium (Cd) contamination is a global environmental challenge that threatens human food security. Lime combined with five different organic materials (rape seed cake, mushroom residue, straw, sawdust, and corn cobs) (LOM) at application ratios of 1:1 and lime sawdust combined with nitro-compound fertilizer (Ca(NO3)2·4H2O, KH2PO4, KNO3) (LSF) at different application rates were applied to Cd-contaminated soil. The present study investigates the effects of these organic–inorganic compound amendments on Cd bioavailability in soil, and Cd uptake and accumulation by edible Artemisia selengensis parts. A. selengensis was cultivated for three consecutive seasons in Cd-contaminated soil. LOM and LSF treatments obviously reduced the uptake and accumulation of Cd. Compared with the control soil, contents of Cd in edible parts of A. selengensis decreased by 19.26–33.33% and 26.67–32.78% in the first season, 18.60–32.79% and 18.37–32.79% in the second season, and 20.45–40.68% and 34.32–37.27% in the third season, respectively. The addition of Lime + Mushroom Residue and 70% Nitro-compound Fertilizer + Lime + Sawdust most significantly reduced Cd concentrations in the edible parts of the third A. selengensis season. LOM and LSF application increased soil pH and improved soil fertility, including available nitrogen, available phosphorus, available potassium, organic matter, and cation exchange capacity. Lime + Mushroom Residue improved plant yield the most. In addition, Lime + Mushroom Residue and 70% Nitro-compound Fertilizer + Lime + Sawdust had the lowest Cd accumulation and health risk indices, respectively. In conclusion, the Lime + Mushroom Residue and 70% Nitro-compound Fertilizer + Lime + Sawdust amendments significantly reduced health risks, enhanced A. selengensis growth, and promoted sustainable development of arable land under Cd-contaminated soil remediation.
Effect of NaCl on Morphophysiological and Biochemical Responses in Gossypium hirsutum L.
Authors: Shaheen Sabahat , Baber Muhammad , Aslam Sidra , Aslam Seema , Shaheen Mehak , Waheed Raheela , Seo Hyojin , Azhar Muhammad Tehseen
Published: March 2023

Soil salinity is increasing due to several factors such as climate change and areas with uneven rainfall. This increase in level of salinity compelled the cotton breeders to develop a new germplasm that exhibit the suitable for salty soil. This study aimed to determine the salt tolerance of 50 accessions of Gossypium hirsutum in hydroponic conditions having three levels of NaCl, i.e., 0 mM, 150 mM, and 200 mM. The experiment was carried out in a completely randomized design with a factorial arrangement. Morphological, physiological, and biochemical attributes were estimated in these genotypes. The Na+/K+ ratio was determined by dry digestion method. Salt-susceptible and -tolerant genotypes were identified by biplot and cluster analysis. The genotypes showed significant differences for morphophysiological and biochemical parameters. In control, Cyto-515 showed enhanced growth with shoot length (30.20 cm), root length (20.63 cm), fresh shoot weight (2.34 g), and fresh root weight (0.93 g), while under 150 mM and 200 mM salinity levels, MNH-992 had the maximum root length (15.67 cm) and shoot length (24.67 cm). At a 150 mM salinity level, maximum levels of antioxidants were found in Kehkshan and CIM-595, while at a 200 mM salinity level, AA-703, CIM-595, and Kehkshan showed maximum values of antioxidants. The highest Na+/K+ ratio was observed in VH-363 and FH-114, while Kehkshan had lowest Na+/K+ ratio. The biplot analysis revealed that Kehkshan, CIM-595, VH-330, Cyto-178, MNH-992, and Cyto-515 were widely dispersed and distant from the origin, and exhibiting variability for morphophysiological and biochemical traits under the salt stress. In terms of performance across the treatments, accessions MNH-992, Kehkshan, Cyto-515, and CIM-595 performed significantly better. Peroxidase activity, proline contents, H2O2 determination, and Na+/K+ ratio were shown to be useful for the salt tolerance selection criteria. The potential of such salt tolerant accessions (MNH-992, Kehkshan, Cyto-515, and CIM-595) could be assessed after planting in salt affected areas and could be used in breeding programs for the development of diverse salt tolerant new genotypes of upland cotton.
Short-Term Evapotranspiration Forecasting of Rubber (Hevea brasiliensis) Plantations in Xishuangbanna, Southwest China
Authors: Ling Zhen , Shi Zhengtao , Xia Tiyuan , Gu Shixiang , Liang Jiaping , Xu Chong-Yu
Published: March 2023

Rubber (Hevea brasiliensis) plantations have high water consumption through evapotranspiration, which can contribute to water scarcity. In addition, there is a lack of spatial observation data and estimation methods for evapotranspiration (ET) for rubber plantations. To alleviate the water stress of expanding rubber plantations caused by seasonal drought in Xishuangbanna, Southwest China, an up to 7 days crop evapotranspiration (ETc) forecast method, “Kc-ET0” for rubber plantations with limited meteorological data, was proposed by using rubber crop coefficient Kc and public weather forecasts. The results showed that the average absolute error (MAE) of forecasted ETc was 0.68 mm d−1, the root mean square error (RMSE) was 0.85 mm d−1, and the average correlation coefficient (R) was 0.69 during the rainy season, while during the dry season these metrics were 0.52 mm d−1, 0.68 mm d−1, and 0.85, respectively. The accuracy of ETc forecast in the dry season was higher. Additionally, Kc was the main factor influencing the accuracy of rubber plantations ETc forecast, while the accuracy of the temperature forecast and the chosen Hargreaves-Samani (HS) model were also considerable. Our results suggested that the “Kc-ET0” short-term rubber plantation ETc forecasting method shows good performance and acceptable accuracy, especially in the dry season. The study provides an important basis for creating ET-based irrigation scheduling for improving regional-scale water management in high water consumption rubber plantations.
Soil-to-Wheat Transfer of Heavy Metals Depending on the Distance from the Industrial Zone
Authors: Mickovski Stefanović Violeta , Roljević Nikolić Svetlana , Matković Stojšin Mirela , Majstorović Helena , Petreš Mladen , Cvikić Dejan , Racić Gordana
Published: March 2023

The accumulation of heavy metals in the environment is one of the most significant environmental problems due to the potential risk to human and animal health. The aim of this study was to analyze the influence of the distance from the industrial zone on the heavy metal content in the soil and vegetative parts of wheat. A field experiment with four wheat genotypes was conducted in the area of the city of Pancevo, Serbia, at three locations at different distances from the industrial zone. By atomic absorption spectrophotometry (AAS), concentrations of five heavy metals (Zn, Pb, Cr, Cu, and Cd) were determined in the soil and wheat. The highest total content of Zn, Cr, Cu, and Cd in the soil (72.5, 27.3, 26.2, and 0.3 mg kg−1, respectively) was found at the location closest to the industrial zone, while the highest content of Pb (28.9 mg kg−1) was recorded at a location that is in the immediate vicinity of a road. Heatmap correlations and PCA analysis show a significant relationship between the content of heavy metals in the soil and the plant. Genotype Pobeda had the lowest content of Cr, Cu, and Cd in the root and the lowest content of all the analyzed heavy metals in the stem. The highest translocation factor of heavy metals was found in the genotype Apache, which had the highest content of Pb, Cr, and Cu in the stem. The highest heavy metal bioaccumulation and translocation were established for Cd content (0.86 and 1.93). The obtained results indicate a potential ecological risk in the immediate vicinity of the industrial zone, while the difference in the accumulation of heavy metals between the studied genotypes opens new aspects for breeding programs.
Influence of Crop Residue Management and Soil Tillage Method on Reducing the Carbon Footprint of Winter Wheat Production in the Salt-Affected Arable Land in the North China Plain
Authors: Li Binbin , Wu Lanfang , Zhu Wanxue , Qiao Chunlian , Zhang Jin , He Wenping
Published: March 2023

Salt-affected arable land is distributed widely in China, especially in the North China Plain. Crop residue management under appropriate tillage is critical to improving salt-affected soil organic carbon and reducing the carbon footprint. This study conducted four-year field experiments including two treatments (residue incorporated into soil with plough tillage (CT+); residue mulching with no-till (NT+)) in two sites (non-saline soil and salt-affected soil); the carbon footprint of wheat production was analyzed by life cycle assessment. The results showed that the carbon footprint of wheat production in the salt-affected soil was significantly larger than that in the non-saline soil, because the salt-affected soil exhibited higher N2O emission than the non-saline soil. CT+ has lower carbon footprint than the NT+, mainly due to the lower N2O emission and higher carbon sequestration in the CT+ compared to NT+. As for the salt-affected soil, the largest contributor of the carbon footprint per unit area was soil N2O emission, with a relative contribution of 40%; the largest contributor of the carbon footprint per unit yield was carbon sequestration, with a relative importance of 47–50%. Our results indicated that wheat production in salt-affected land has a high carbon footprint, while it can be decreased by incorporating crop residue into the soil under the plough tillage.