bluuebunny/crossref_metadata_2025 huggingface dataset:
Lights and Shadows on the Therapeutic Use of Antimicrobial Peptides
Authors: Bellotti Denise , Remelli Maurizio
Published: July 2022

The emergence of antimicrobial-resistant infections is still a major concern for public health worldwide. The number of pathogenic microorganisms capable of resisting common therapeutic treatments are constantly increasing, highlighting the need of innovative and more effective drugs. This phenomenon is strictly connected to the rapid metabolism of microorganisms: due to the huge number of mutations that can occur in a relatively short time, a colony can “adapt” to the pharmacological treatment with the evolution of new resistant species. However, the shortage of available antimicrobial drugs in clinical use is also caused by the high costs involved in developing and marketing new drugs without an adequate guarantee of an economic return; therefore, the pharmaceutical companies have reduced their investments in this area. The use of antimicrobial peptides (AMPs) represents a promising strategy for the design of new therapeutic agents. AMPs act as immune defense mediators of the host organism and show a poor ability to induce antimicrobial resistance, coupled with other advantages such as a broad spectrum of activity, not excessive synthetic costs and low toxicity of both the peptide itself and its own metabolites. It is also important to underline that many antimicrobial peptides, due to their inclination to attack cell membranes, have additional biological activities, such as, for example, as anti-cancer drugs. Unfortunately, they usually undergo rapid degradation by proteolytic enzymes and are characterized by poor bioavailability, preventing their extensive clinical use and landing on the pharmaceutical market. This review is focused on the strength and weak points of antimicrobial peptides as therapeutic agents. We give an overview on the AMPs already employed in clinical practice, which are examples of successful strategies aimed at overcoming the main drawbacks of peptide-based drugs. The review deepens the most promising strategies to design modified antimicrobial peptides with higher proteolytic stability with the purpose of giving a comprehensive summary of the commonly employed approaches to evaluate and optimize the peptide potentialities.
Detecting Emotions from Illustrator Gestures—The Italian Case
Authors: Fundarò Daniele , Gentile Vito , Milazzo Fabrizio , Sorce Salvatore
Published: July 2022

The evolution of computers in recent years has given a strong boost to research techniques aimed at improving human–machine interaction. These techniques tend to simulate the dynamics of the human–human interaction process, which is based on our innate ability to understand the emotions of other humans. In this work, we present the design of a classifier to recognize the emotions expressed by human beings, and we discuss the results of its testing in a culture-specific case study. The classifier relies exclusively on the gestures people perform, without the need to access additional information, such as facial expressions, the tone of a voice, or the words spoken. The specific purpose is to test whether a computer can correctly recognize emotions starting only from gestures. More generally, it is intended to allow interactive systems to be able to automatically change their behaviour based on the recognized mood, such as adapting the information contents proposed or the flow of interaction, in analogy to what normally happens in the interaction between humans. The document first introduces the operating context, giving an overview of the recognition of emotions and the approach used. Subsequently, the relevant bibliography is described and analysed, highlighting the strengths of the proposed solution. The document continues with a description of the design and implementation of the classifier and of the study we carried out to validate it. The paper ends with a discussion of the results and a short overview of possible implications.
The Appraisal Principle in Multimedia Learning: Impact of Appraisal Processes, Modality, and Codality
Authors: Zumbach Joerg , Zeitlhofer Ines , Mann Bettina , Hoermann Sandra , Reisenhofer Birgit
Published: July 2022

This paper presents two experiments examining the influences of media-specific appraisal and attribution on multimedia learning. The first experiment compares four different versions of learning material (text, text with images, animation with text, and animation with audio). Results reveal that the attributed type of appraisal, (i.e., the subjective impression of whether a medium is easy or difficult to learn with) impacts invested mental effort and learning outcomes. Though there was no evidence for the modality effect in the first experiment, we were able to identify it in a second study. We were also able to replicate appraisal and attribution findings from study 1 in study 2: if media appraisal leads to the result that learning with a specific medium is difficult, more mental effort will be invested in information processing. Consequently, learning outcomes are better, and learners are more likely to attribute knowledge acquisition to their own abilities. Outcomes also indicate that the modality effect can be explained by avoidance of split-attention rather than modality-specific information processing in working memory.
Synthesis and Characterization of Monodispersed Spherical Calcium Oxide and Calcium Carbonate Nanoparticles via Simple Pyrolysis
Authors: Atchudan Raji , Perumal Suguna , Joo Jin , Lee Yong Rok
Published: July 2022

In this study, calcium carbonate nanoparticles (CCNPs) and calcium oxide nanoparticles (CONPs) are synthesized by the carbonization/calcination of calcium oleate. CONPs are an essential inorganic material, and they are used as catalysts and as effective chemisorbents for toxic gases. CCNPs are widely used in plastics, printing ink, and medicines. Here, calcium oleate is used as a starting material for the preparation of CCNPs and CONPs. This calcium oleate is prepared from calcium hydroxide and oleic acid in ethanol under mild reflux conditions. The effect of the calcination temperature of calcium oleate is examined during the synthesis of CCNPs and CONPs. By simple carbonization/calcination, calcite-type CCNPs and CONPs are prepared at <550 °C and >600 °C, respectively. The synthesized nanomaterials are analyzed by various physicochemical characterization techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA) with derivative thermogravimetry (DTG), and scanning electron microscopy (SEM) with energy dispersive X-ray analysis. An X-ray diffractometer and the Scherrer formula are used to analyze the crystalline phase and crystallite size of prepared nanoparticles. TGA techniques confirm the thermal stability of the calcium oleate, CCNPs, and CONPs. The SEM analysis illustrates the dispersive behavior and cubic/spherical morphologies of CCNPs/CONPs. Furthermore, the obtained results are compared to the CCNP and CONP samples prepared using calcium hydroxide. As a result, the carbonization/calcination of calcium oleate produces monodispersed CONPs, which are then compared to the CONPs from calcium hydroxide. Additionally, from calcium oleate, CONPs can be prepared on a large scale in a cheap, convenient way, using simple equipment which can be applied in various applications.
Preparation and Characterization of Screen-Printed Cu2S/PEDOT:PSS Hybrid Films for Flexible Thermoelectric Power Generator
Authors: Zhao Junmei , Zhao Xiaolong , Guo Rui , Zhao Yaxin , Yang Chenyu , Zhang Liping , Liu Dan , Ren Yifeng
Published: July 2022

In recent years, flexible thermoelectric generators(f-TEG), which can generate electricity by environmental temperature difference and have low cost, have been widely concerned in self-powered energy devices for underground pipe network monitoring. This paper studied the Cu2S films by screen-printing. The effects of different proportions of p-type Cu2S/poly 3,4-ethylene dioxythiophene-polystyrene sulfonate (PEDOT:PSS) mixture on the thermoelectric properties of films were studied. The interfacial effect of the two materials, forming a superconducting layer on the surface of Cu2S, leads to the enhancement of film conductivity with the increase of PEDOT:PSS. In addition, the Seebeck coefficient decreases with the increase of PEDOT:PSS due to the excessive bandgap difference between the two materials. When the content ratio of Cu2S and PEDOT:PSS was 1:1.2, the prepared film had the optimal thermoelectric performance, with a maximum power factor (PF) of 20.60 μW·m−1·K−1. The conductivity reached 75% of the initial value after 1500 bending tests. In addition, a fully printed Te-free f-TEG with a fan-shaped structure by Cu2S and Ag2Se was constructed. When the temperature difference (ΔT) was 35 K, the output voltage of the f-TEG was 33.50 mV, and the maximum power was 163.20 nW. Thus, it is envisaged that large thermoelectric output can be obtained by building a multi-layer stacking f-TEG for continuous self-powered monitoring.
Crystalline Biomimetic Calcium Phosphate Coating on Mini-Pin Implants to Accelerate Osseointegration and Extend Drug Release Duration for an Orthodontic Application
Authors: Li Menghong , Wu Gang , Wang Mingjie , Hunziker Ernst B. , Liu Yuelian
Published: July 2022

Miniscrew implants (MSIs) have been widely used as temporary anchorage devices in orthodontic clinics. However, one of their major limitations is the relatively high failure rate. We hypothesize that a biomimetic calcium phosphate (BioCaP) coating layer on mini-pin implants might be able to accelerate the osseointegration, and can be a carrier for biological agents. A novel mini-pin implant to mimic the MSIs was used. BioCaP (amorphous or crystalline) coatings with or without the presence of bovine serum albumin (BSA) were applied on such implants and inserted in the metaphyseal tibia in rats. The percentage of bone to implant contact (BIC) in histomorphometric analysis was used to evaluate the osteoconductivity of such implants from six different groups (n=6 rats per group): (1) no coating no BSA group, (2) no coating BSA adsorption group, (3) amorphous BioCaP coating group, (4) amorphous BioCaP coating-incorporated BSA group, (5) crystalline BioCaP coating group, and (6) crystalline BioCaP coating-incorporated BSA group. Samples were retrieved 3 days, 1 week, 2 weeks, and 4 weeks post-surgery. The results showed that the crystalline BioCaP coating served as a drug carrier with a sustained release profile. Furthermore, the significant increase in BIC occurred at week 1 in the crystalline coating group, but at week 2 or week 4 in other groups. These findings indicate that the crystalline BioCaP coating can be a promising surface modification to facilitate early osseointegration and increase the success rate of miniscrew implants in orthodontic clinics.
Fabrication of Nitrogen-Doped Reduced Graphene Oxide Modified Screen Printed Carbon Electrode (N-rGO/SPCE) as Hydrogen Peroxide Sensor
Authors: Ahmad Khursheed , Kim Haekyoung
Published: July 2022

In recent years, the electrochemical sensing approach has attracted electrochemists because of its excellent detection process, simplicity, high sensitivity, cost-effectiveness, and high selectivity. In this study, we prepared nitrogen doped reduced graphene oxide (N-rGO) and characterized it using various advanced techniques such as XRD, SEM, EDX, Raman, and XPS. Furthermore, we modified the active surface of a screen printed carbon electrode (SPCE) via the drop-casting of N-rGO. This modified electrode (N-rGO/SPCE) exhibited an excellent detection limit (LOD) of 0.83 µM with a decent sensitivity of 4.34 µAµM−1cm−2 for the detection of hydrogen peroxide (H2O2). In addition, N-rGO/SPCE also showed excellent selectivity, repeatability, and stability for the sensing of H2O2. Real sample investigations were also carried out that showed decent recovery.
Fabrication of Zn-Cu-Ni Ternary Oxides in Nanoarrays for Photo-Enhanced Pseudocapacitive Charge Storage
Authors: Xu Ruitong , Pan Jun , Wu Bo , Li Yangguang , Wang Hong-En , Zhu Ting
Published: July 2022

To meet the increasing demands of energy consumption, sustainable energy sources such as solar energy should be better employed to promote electrochemical energy storage. Herein, we fabricated a bifunctional photoelectrode composed of copper foam (CF)-supported zinc-nickel-copper ternary oxides in nanoarrays (CF@ZnCuNiOx NAs) to promote photo-enhanced pseudocapacitive charge storage. The as-fabricated CF@ZnCuNiOx NAs have shown both photosensitive and pseudocapacitive characteristics, demonstrating a synergistic effect on efficient solar energy harvest and conversion. As a result, a high areal specific capacitance of 2741 mF cm−2 (namely 418 μAh cm−2) under light illumination can be calculated at 5 mA cm−2, which delivered photo-enhancement of 38.3% compared to that obtained without light. In addition, the photoelectric and photothermal effects of the light energy on pseudocapacitive charge storage have been preliminarily studied and compared. This work may provide some evidence on the different mechanisms of photoelectric/thermal conversion for developing solar-driven energy storage devices.
3D NAND Flash Memory Based on Double-Layer NC-Si Floating Gate with High Density of Multilevel Storage
Authors: Yu Xinyue , Ma Zhongyuan , Shen Zixiao , Li Wei , Chen Kunji , Xu Jun , Xu Ling
Published: July 2022

As a strong candidate for computing in memory, 3D NAND flash memory has attracted great attention due to the high computing efficiency, which outperforms the conventional von-Neumann architecture. To ensure 3D NAND flash memory is truly integrated in the computing in a memory chip, a new candidate with high density and a large on/off current ratio is now urgently needed. Here, we first report that 3D NAND flash memory with a high density of multilevel storage can be realized in a double-layered Si quantum dot floating-gate MOS structure. The largest capacitance–voltage (C-V) memory window of 6.6 V is twice as much as that of the device with single-layer nc-Si quantum dots. Furthermore, the stable memory window of 5.5 V can be kept after the retention time of 105 s. The obvious conductance–voltage (G-V) peaks related to the charging process can be observed, which further confirms that the multilevel storage can be realized in double-layer Si quantum dots. Moreover, the on/off ratio of 3D NAND flash memory with a nc-Si floating gate can reach 104, displaying the characteristic of a depletion working mode of an N-type channel. The memory window of 3 V can be maintained after 105 P/E cycles. The programming and erasing speed can arrive at 100 µs under the bias of +7 V and −7 V. Our introduction of double-layer Si quantum dots in 3D NAND float gating memory supplies a new way to the realization of computing in memory.
Coupled Effects of Using Magnetic Field, Rotation and Wavy Porous Layer on the Forced Convection of Hybrid Nanoliquid Flow over 3D-Backward Facing Step
Authors: Ghachem Kaouther , Selimefendigil Fatih , Alshammari Badr M. , Maatki Chemseddine , Kolsi Lioua
Published: July 2022

In the present study, the effects of using a corrugated porous layer on the forced convection of a hybrid nanofluid flow over a 3D backward facing step are analyzed under the coupled effects of magnetic field and surface rotation. The thermal analysis is conducted for different values of the Reynolds number (Re between 100 and 500), the rotational Reynolds number (Rew between 0 and 2000), the Hartmann number (Ha between 0 and 15), the permeability of the porous layer (the Darcy number, Da between 10−5 and 10−2) and the amplitude (ax between 0.01 ap and 0.7 ap) and wave number (N between 1 and 16) of the porous layer corrugation. When rotations are activated, the average Nusselt number (Nu) and pressure coefficient values rise, while the increment of the latter is less. The increment in the average Nu is higher for the case with a higher permeability of the layer. When the corrugation amplitude and wave number are increased, favorable impacts of the average Nu are observed, but at the same time pressure coefficients are increased. Successful thermal performance estimations are made by using a neural-based modeling approach with a four input-two output system.
Long Non-Coding RNAs in Cardiac and Pulmonary Fibroblasts and Fibrosis
Authors: Ilieva Mirolyuba , Uchida Shizuka
Published: July 2022

The cardiopulmonary system delivers oxygen throughout the body via blood circulation. It is an essential part of the body to sustain the lives of organisms. The integral parts of the cardiopulmonary system—the heart and lungs—are constantly exposed to damaging agents (e.g., dust, viruses), and can be greatly affected by injuries caused by dysfunction in tissues (e.g., myocardial infarction). When damaged, mesenchymal cells, such as fibroblasts, are activated to become myofibroblasts to initiate fibrosis as part of a regenerative mechanism. In diseased states, the excess accumulation of extracellular matrices secreted by myofibroblasts results in further dysfunction in the damaged organs. These fibrotic tissues cannot easily be removed. Thus, there is a growing interest in understanding the fibrotic process, as well as finding biomolecules that can be targets for slowing down or potentially stopping fibrosis. Among these biomolecules, the interest in studying long non-coding RNAs (lncRNAs; any non-protein-coding RNAs longer than 200 nucleotides) has intensified in recent years. In this commentary, we summarize the current status of lncRNA research in the cardiopulmonary system by focusing on cardiac and pulmonary fibrosis.
Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway
Authors: Wang Yu , Gao Xuyang , Chen Xiaoyou , Li Qiang , Li Xinrui , Zhao Junxing
Published: July 2022

Seabuckthorn possesses various bioactive compounds and exhibits several positive pharmacological activities. The present trial aims to determine the effect of seabuckthorn powder intake on high-fat diet (HFD)-induced obesity prevention in mice. The results suggest that seabuckthorn powder intake decreased body weight, fat mass, and circulating lipid levels, and improved insulin sensitivity in HFD-fed mice. Moreover, dietary seabuckthorn powder alleviated hepatic steatosis and hepatic lipid accumulation induced by the HFD. Furthermore, seabuckthorn exhibited obvious anti-inflammatory capacity in white adipose tissue (WAT) by regulating the abundance of inflammation-related cytokines, such as interleukins 4, 6, and 10; tumor necrosis factor α; and interferon-γ. More importantly, dietary seabuckthorn powder promoted a thermogenic program in BAT and induced beige adipocyte formation in iWAT in HFD-fed mice. Interestingly, we found that seabuckthorn powder effectively restored AMPK and SIRT1 activities in both BAT and iWAT in HFD-fed mice. Collectively, these results potentiate the application of seabuckthorn powder as a nutritional intervention strategy to prevent obesity and related metabolic diseases by promoting thermogenesis in BAT and improving beige adipocyte formation in WAT.
The Effect of Chronotype on Addictive Eating Behavior and BMI among University Students: A Cross-Sectional Study
Authors: Arslan Müge , Ayhan Nurcan Yabancı , Çolak Hatice , Sarıyer Esra Tansu , Çevik Ekin
Published: July 2022

The aim of this study was to examine the effect of chronotype on addictive eating behavior and BMI in university students. A questionnaire on their sociodemographic characteristics and eating habits, as well as the three-factor eating questionnaire (TFEQ) and the morningness-eveningness questionnaire (MEQ), were given to students at Üsküdar University. Data were analyzed with IBM SPSS 26.0. This study included a total of 850 students, 46.8% of whom were male and 53.2% were female. The mean BMI was 22.94 ± 3.30 kg/m2. As the BMI values of students increased, there was a decrease of 25.6% in the TFEQ scores, regardless of gender (s = −0.256; p < 0.001), and an increase of 10.6% in their chronotype scores, meaning that the students tended to be morning types (s = 0.106; p < 0.01). A significantly negative, very weak correlation was found between the students’ TFEQ and MEQ scores (s = −0.123; p < 0.001). The students’ BMI (p < 0.01) and TFEQ scores (p < 0.001) were affected by their MEQ scores in a statistically significant way. It was found that each 1-point increase in the MEQ score led to a 4.0% increase in the BMI score and a 15.8% decrease in the TFEQ score. It was observed that the morning-type students tended to be obese in terms of their BMI, and these students demonstrated fewer addictive eating behaviors.
Epicardial Adipose Tissue: A Novel Potential Imaging Marker of Comorbidities Caused by Chronic Inflammation
Authors: Tarsitano Maria Grazia , Pandozzi Carla , Muscogiuri Giuseppe , Sironi Sandro , Pujia Arturo , Lenzi Andrea , Giannetta Elisa
Published: July 2022

The observation of correlations between obesity and chronic metabolic and cardiovascular diseases has led to the emergence of strong interests in “adipocyte biology”, in particular in relation to a specific visceral adipose tissue that is the epicardial adipose tissue (EAT) and its pro-inflammatory role. In recent years, different imaging techniques frequently used in daily clinical practice have tried to obtain an EAT quantification. We provide a useful update on comorbidities related to chronic inflammation typical of cardiac adiposity, analyzing how the EAT assessment could impact and provide data on the patient prognosis. We assessed for eligibility 50 papers, with a total of 10,458 patients focusing the review on the evaluation of EAT in two main contexts: cardiovascular and metabolic diseases. Given its peculiar properties and rapid responsiveness, EAT could act as a marker to investigate the basal risk factor and follow-up conditions. In the future, EAT could represent a therapeutic target for new medications. The assessment of EAT should become part of clinical practice to help clinicians to identify patients at greater risk of developing cardiovascular and/or metabolic diseases and to provide information on their clinical and therapeutic outcomes.
Motivation and Limiting Factors for Adherence to Weight Loss Interventions among Patients with Obesity in Primary Care
Authors: Trujillo-Garrido Nuria , Santi-Cano María J.
Published: July 2022

The cornerstones of obesity management are diet, physical activity and behavioral change. However, there is considerable scientific evidence that lifestyle interventions to treat obesity are rarely implemented in primary care. The aim of this study is to analyze motivation to lose weight among patients with obesity, the resources implemented by primary care centers to promote behavioral change and the limiting factors reported by the patients themselves when attempting to lose weight. A total of 209 patients diagnosed with obesity were interviewed. The variables were obtained from both electronic clinical records (sex, age, BMI, diagnosis of metabolic syndrome and records of activities prescribed to promote behavioral change) and a self-administered personal questionnaire. A total of 67.5% of the respondents reported not having sufficient motivation to adhere to a weight loss program. Records of behavioral change activities were identified in only 3% of the clinical records reviewed. The barriers to adherence to diet and exercise plans most frequently mentioned by patients were not having a prescribed diet (27.8%), joint pain (17.7%), getting tired or bored of dieting (14.8%) and laziness (11.5%). Both the high percentage of patients reporting insufficient motivation to lose weight and the barriers to weight loss identified suggest that patients feel the need to improve their motivation, which should be promoted through primary care.
Reactive Oxygen Species Signaling Pathways: Arbiters of Evolutionary Conflict?
Authors: Blackstone Neil W.
Published: July 2022

In the history of life, cooperation between biological units has led to increased complexity, e.g., eukaryotic cells and multicellular organisms. Cooperation requires limiting the gains of “defectors” in favor of the cooperative higher-level unit. Early in an evolutionary transition, bioenergetics and reactive oxygen species (ROS) may play a large role in managing these evolutionary conflicts. Chemiosmosis can be thought of as a poorly insulated wire—when supply exceeds demand, electrons are cast off and can form ROS. ROS signaling may thus lead to the dispersal of the excess products into the environment. These products may lead to groups and the formation of higher-level units that can subsequently be targeted by selection. Examining modern symbioses such as those between corals and photosynthetic dinoflagellates provides useful insight in this context. While ROS are an important factor in coral bleaching, little is known of the function of ROS under other circumstances, although some data suggest that ROS may modulate cooperation. ROS may have functioned similarly in the origin of eukaryotes, involving chemiosmotic mitochondria and chloroplasts. ROS may act as “arbiters” of evolutionary conflict, leading to cooperation via signaling pathways that favor the emergence of the higher-level unit.
Opportunities and Challenges for In Silico Drug Discovery at Delta Opioid Receptors
Authors: Meqbil Yazan , van Rijn Richard
Published: July 2022

The delta opioid receptor is a Gi-protein-coupled receptor (GPCR) with a broad expression pattern both in the central nervous system and the body. The receptor has been investigated as a potential target for a multitude of significant diseases including migraine, alcohol use disorder, ischemia, and neurodegenerative diseases. Despite multiple attempts, delta opioid receptor-selective molecules have not been translated into the clinic. Yet, the therapeutic promise of the delta opioid receptor remains and thus there is a need to identify novel delta opioid receptor ligands to be optimized and selected for clinical trials. Here, we highlight recent developments involving the delta opioid receptor, the closely related mu and kappa opioid receptors, and in the broader area of the GPCR drug discovery research. We focus on the validity and utility of the available delta opioid receptor structures. We also discuss the increased ability to perform ultra-large-scale docking studies on GPCRs, the rise in high-resolution cryo-EM structures, and the increased prevalence of machine learning and artificial intelligence in drug discovery. Overall, we pose that there are multiple opportunities to enable in silico drug discovery at the delta opioid receptor to identify novel delta opioid modulators potentially with unique pharmacological properties, such as biased signaling.
Tumor-Derived Membrane Vesicles: A Promising Tool for Personalized Immunotherapy
Authors: Xu Jiabin , Cao Wenqiang , Wang Penglai , Liu Hong
Published: July 2022

Tumor-derived membrane vesicles (TDMVs) are non-invasive, chemotactic, easily obtained characteristics and contain various tumor-borne substances, such as nucleic acid and proteins. The unique properties of tumor cells and membranes make them widely used in drug loading, membrane fusion and vaccines. In particular, personalized vectors prepared using the editable properties of cells can help in the design of personalized vaccines. This review focuses on recent research on TDMV technology and its application in personalized immunotherapy. We elucidate the strengths and challenges of TDMVs to promote their application from theory to clinical practice.
Dexamethasone Increases the Anesthetic Success in Patients with Symptomatic Irreversible Pulpitis: A Meta-Analysis
Authors: Franco-de la Torre Lorenzo , Gómez-Sánchez Eduardo , Serafín-Higuera Nicolás Addiel , Alonso-Castro Ángel Josabad , López-Verdín Sandra , Molina-Frechero Nelly , Granados-Soto Vinicio , Isiordia-Espinoza Mario Alberto
Published: July 2022

Inferior alveolar nerve block (IANB) has a high failure rate in subjects with symptomatic irreversible pulpitis (SIP). It has been suggested that drugs with anti-inflammatory activity could improve the efficacy of the anesthetic used for IANB. The aim of this study was to assess the effect of dexamethasone on the success of dental anesthesia in patients with SIP. An information search was performed using PubMed and Google Scholar. The risk of bias of the included studies was evaluated with the Cochrane Collaboration’s risk-of-bias tool. The anesthetic success rate, pain intensity (VAS), and adverse effects were extracted. Data were analyzed using the Mantel–Haenszel test and odds ratio or the inverse variance and standardized mean difference. Dexamethasone increased the anesthetic success in comparison with placebo (n = 502; p < 0.001; OR = 2.59; 95% CIs: 1.46 to 4.59). Moreover, patients who were given dexamethasone had lower pain scores at 6 h (n = 302; p < 0.001; MD= −1.43; 95% CIs: −2.28 to −0.58), 12 h (n = 302; p < 0.0001; MD = −1.65; 95% CIs: −2.39 to −0.92), and 24 h (n = 302; p < 0.0008; MD = −1.27; 95% CIs: −2.01 to −0.53) when compared with placebo. In conclusion, the systemic administration of dexamethasone increases the anesthetic success rate and improves pain management in patients with SIP.
Morphological Changes, Antibacterial Activity, and Cytotoxicity Characterization of Hydrothermally Synthesized Metal Ions-Incorporated Nanoapatites for Biomedical Application
Authors: Huang Ssu-Meng , Liu Shih-Ming , Chen Wen-Cheng , Ko Chia-Ling , Shih Chi-Jen , Chen Jian-Chih
Published: July 2022

The objective of this study was to prepare hydroxyapatite (HA) with potential antibacterial activity against gram-negative and gram-positive bacteria by incorporating different atomic ratios of Cu2+ (0.1–1.0%), Mg2+ (1.0–7.0%), and Zn2+ (1.0–7.0%) to theoretically replace Ca2+ ions during the hydrothermal synthesis of grown precipitated HA nanorods. This study highlights the role of comparing different metal ions on synthetic nanoapatite in regulating the antibacterial properties and toxicity. The comparisons between infrared spectra and between diffractograms have confirmed that metal ions do not affect the formation of HA phases. The results show that after doped Cu2+, Mg2+, and Zn2+ ions replace Ca2+, the ionic radius is almost the same, but significantly smaller than that of the original Ca2+ ions, and the substitution effect causes the lattice distance to change, resulting in crystal structure distortion and reducing crystallinity. The reduction in the length of the nanopatites after the incorporation of Cu2+, Mg2+, and Zn2+ ions confirmed that the metal ions were mainly substituted during the growth of the rod-shape nanoapatite Ca2+ distributed along the longitudinal site. The antibacterial results show that nanoapatite containing Cu2+ (0.1%), Mg2+ (3%), and Zn2+ (5–7%) has obvious and higher antibacterial activity against gram-positive bacteria Staphylococcus aureus within 2 days. The antibacterial effect against the gram-negative bacillus Escherichia coli is not as pronounced as against Staphylococcus aureus. The antibacterial effect of Cu2+ substituted Ca2+ with an atomic ratio of 0.1~1.0% is even better than that of Mg2+- and Zn2+- doped with 1~7% groups. In terms of cytotoxicity, nanoapatites with Cu2+ (~0.2%) exhibit cytotoxicity, whereas Mg2+- (1–5%) and Zn2+- (~1%) doped nanoapatites are biocompatible at low concentrations but become cytotoxic as ionic concentration increases. The results show that the hydrothermally synthesized nanoapatite combined with Cu2+ (0.2%), Mg2+ (3%), and Zn2+ (3%) exhibits low toxicity and high antibacterial activity, which provides a good prospect for bypassing antibiotics for future biomedical applications.
Novel Treatments for Age-Related Macular Degeneration: A Review of Clinical Advances in Sustained Drug Delivery Systems
Authors: Jiménez-Gómez Yolanda , Alba-Molina David , Blanco-Blanco Mario , Pérez-Fajardo Lorena , Reyes-Ortega Felisa , Ortega-Llamas Laura , Villalba-González Marta , Fernández-Choquet de Isla Ignacio , Pugliese Francisco , Stoikow Indira , González-Andrades Miguel
Published: July 2022

In recent years, the number of patients with ocular diseases is increasing as a consequence of population aging. Among them, one of the most common is the age-related macular degeneration (AMD), a condition that leads to vision loss if it is not treated. AMD is a multifactorial disorder with two advanced forms, dry and neovascular AMD. Currently, although there is no approved therapy that significantly impacts dry AMD progression, several pharmacologic therapies exist for neovascular AMD. Notwithstanding, evidence suggests a suboptimal result in a high number of patients receiving these therapeutic options. Consequently, finding effective strategies is not only a still unmet medical need in dry AMD but also in neovascular AMD. This underlines the need for new drug delivery technologies that can improve the pharmacological action and drug concentration at the target sites. In this regard, sustained drug delivery systems are presented as the most promising therapeutic options in AMD patients. This review summarized the pathogenesis and the current treatment options for AMD, focusing on the emerging ocular sustained drug delivery approaches undergoing clinical trials.
Preclinical Evaluation of a New Format of 68Ga- and 111In-Labeled Affibody Molecule ZIGF-1R:4551 for the Visualization of IGF-1R Expression in Malignant Tumors Using PET and SPECT
Authors: Liu Yongsheng , Yu Shengze , Xu Tianqi , Bodenko Vitalina , Orlova Anna , Oroujeni Maryam , Rinne Sara S. , Tolmachev Vladimir , Vorobyeva Anzhelika , Gräslund Torbjörn
Published: July 2022

The Insulin-like growth factor-1 receptor (IGF-1R) is a molecular target for several monoclonal antibodies undergoing clinical evaluation as anticancer therapeutics. The non-invasive detection of IGF-1R expression in tumors might enable stratification of patients for specific treatment and improve the outcome of both clinical trials and routine treatment. The affibody molecule ZIGF-1R:4551 binds specifically to IGF-1R with subnanomolar affinity. The goal of this study was to evaluate the 68Ga and 111In-labeled affibody construct NODAGA-(HE)3-ZIGF-1R:4551 for the imaging of IGF-1R expression, using PET and SPECT. The labeling was efficient and provided stable coupling of both radionuclides. The two imaging probes, [68Ga]Ga-NODAGA-(HE)3-ZIGF-1R:4551 and [111In]In-NODAGA-(HE)3-ZIGF-1R:4551, demonstrated specific binding to IGF-1R-expressing human cancer cell lines in vitro and to IGF-1R-expressing xenografts in mice. Preclinical PET and SPECT/CT imaging demonstrated visualization of IGF-1R-expressing xenografts already one hour after injection. The tumor-to-blood ratios at 3 h after injection were 7.8 ± 0.2 and 8.0 ± 0.6 for [68Ga]Ga-NODAGA-(HE)3-ZIGF-1R:4551 and [111In]In-NODAGA-(HE)3-ZIGF-1R:4551, respectively. In conclusion, a molecular design of the ZIGF-1R:4551 affibody molecule, including placement of a (HE)3-tag on the N-terminus and site-specific coupling of a NODAGA chelator on the C-terminus, provides a tracer with improved imaging properties for visualization of IGF-1R in malignant tumors, using PET and SPECT.
Gastro-Resistant Microparticles Produced by Spray-Drying as Controlled Release Systems for Liposoluble Vitamins
Authors: Terracina Francesca , Caruana Roberto , Bonomo Francesco Paolo , Montalbano Francesco , Licciardi Mariano
Published: July 2022

In the present study, gastro-resistant microparticles (MPs) were produced using the spray-drying technique as controlled-release systems for some model liposoluble vitamins, including retinyl-palmitate, retinyl-acetate, β-carotene, cholecalciferol and α-tocopherol. The gastroprotective action of three different gastro-resistant excipients, the anionic methacrylic copolymer (Eudraguard®® Biotic, E1207), the cellulose acetate phthalate (CAP) and whey proteins (WPs), was compared. The latter was used to produce a novel delivery system manufactured with only food-derived components, such as milk, and showed several improvements over the two synthetic gastro-resistant agents. Scanning electron microscopy (SEM) images showed a quite homogeneous spherical shape of all microparticle batches, with an average diameter between 7 and 15 μm. FTIR analysis was used to evaluate the effective incorporation of vitamins within the microparticles and the absence of any degradation to the components of the formulation. The comparison graphs of differential scanning calorimetry (DSC) confirmed that the spray drying technique generates a solid in which the physical interactions between the excipients and the vitamins are very strong. Release studies showed a prominent pH-controlled release and partially a delayed-release profile. Ex vivo permeation studies of retinyl palmitate, retinyl acetate and α-tocopherol revealed greater transmucosal permeation capacity for microparticles produced with the WPs and milk.
Complex Cardinal Numerals and the Strong Minimalist Thesis
Authors: Di Sciullo Anna Maria
Published: July 2022

Different analyses of complex cardinal numerals have been proposed in Generative Grammar. This article provides an analysis of these expressions based on the Strong Minimalist Thesis, according to which the derivations of linguistic expressions are generated by a simple combinatorial operation, applying in accord with principles external to the language faculty. The proposed derivations account for the asymmetrical structure of additive and multiplicative complexes and for the instructions they provide to the external systems for their interpretation. They harmonize with those of coordinate nouns, and thus offer a unified Minimalist account of their core properties. Firstly, the empirical problem addressed is stated. Secondly, the theoretical framework is presented. Thirdly, Minimalist derivations for additive and multiplicative complexes are provided. Fourthly, the proposed derivations are contrasted with derivations not relying on the Strong Minimalist Thesis. Lastly, consequences for linguistic theory are identified as well as questions open to further inquiry.
Photocatalyzed Production of Urea as a Hydrogen–Storage Material by TiO2–Based Materials
Authors: Matamala-Troncoso Felipe , Isaacs Mauricio , Sáez-Navarrete César
Published: July 2022

This review analyzes the photocatalyzed urea syntheses by TiO2–based materials. The most outstanding works in synthesizing urea from the simultaneous photocatalyzed reduction of carbon dioxide and nitrogen compounds are reviewed and discussed. Urea has been widely used in the agricultural industry as a fertilizer. It represents more than 50% of the nitrogen fertilizer market, and its global demand has increased more than 100 times in the last decades. In energy terms, urea has been considered a hydrogen–storage (6.71 wt.%) and ammonia–storage (56.7 wt.%) compound, giving it fuel potential. Urea properties meet the requirements of the US Department of Energy for hydrogen–storage substances, meanly because urea crystalizes, allowing storage and safe transportation. Conventional industrial urea synthesis is energy–intensive (3.2–5.5 GJ ton−1) since it requires high pressures and temperatures, so developing a photocatalyzed synthesis at ambient temperature and pressure is an attractive alternative to conventional synthesis. Due to the lack of reports for directly catalyzed urea synthesis, this review is based on the most prominent works. We provide details of developed experimental set–ups, amounts of products reported, the advantages and difficulties of the synthesis, and the scope of the technological and energetic challenges faced by TiO2–based photocatalyst materials used for urea synthesis. The possibility of scaling photocatalysis technology was evaluated as well. We hope this review invites exploring and developing a technology based on clean and renewable energies for industrial urea production.
Chemical Composition, Antioxidant Potentials, and Calcium Oxalate Anticrystallization Activity of Polyphenol and Saponin Fractions from Argania spinosa L. Press Cake
Authors: El oumari Fatima Ezzahra , Mammate Naima , Imtara Hamada , Lahrichi Anissa , Elhabbani Radouane , El mouhri Ghita , Alqahtani Ali S. , Noman Omar M. , Ibrahim Mansour N. , Grafov Andriy , Bousta Dalila , Sqalli Houssaini Tarik
Published: July 2022

A wide range of biological properties and a potent therapeutic and prophylactic effect on chronic diseases are all present in Argania spinosa L. press cake. The aim of this research is to valorize the anticrystallization properties against calcium oxalate crystals of Argania spinosa L. press cake fractions and identify its bioactive components. Chemical species identification was performed using GC–MS analysis. The turbidimetric model was used to investigate crystallization inhibition in vitro. Infrared spectroscopy technique was used to characterize the synthesized crystals. Furthermore, both DPPH and FRAP methods were used to assess antioxidant activity. The results show that the fractions are equally important in crystallization inhibition percentages of calcium oxalate crystals. For saponin and polyphenol fractions, the inhibition percentages are in the orders of 83.49% and 82.83%, respectively. The results of the antioxidant activity by DPPH method show that the two fractions are equally important in the elimination of free radicals; the inhibition percentages were 77.87 ± 4.21 and 89.92 ± 1.39 for both polyphenols and saponins, respectively. FRAP method showed that the absorbance increases proportionally with concentration, and the absorbance are almost similar for both fractions and reach maximum values in the orders of 0.52 ± 0.07 and 0.42 ± 0.03, respectively, for saponins and polyphenols. These findings demonstrate that both fractions are rich in bioactive chemicals and have an anticrystallization capacity, allowing them to be employed for the curative and prophylactic effects against urolithiasis.
Salicylic Acid Improves Growth and Physiological Attributes and Salt Tolerance Differentially in Two Bread Wheat Cultivars
Authors: Abdi Neila , Van Biljon Angeline , Steyn Chrisna , Labuschagne Maryke Tine
Published: July 2022

Abiotic constraints such as salinity stress reduce cereal production. Salicylic acid is an elicitor of abiotic stress tolerance in plants. The aim of this study was to investigate the effects of salicylic acid on two bread wheat cultivars (SST806 and PAN3497) grown under salt stress (100 and 200 mM NaCl) in the presence and absence of 0.5 mM salicylic acid. The highest salt concentration (200 mM), in both PAN3497 and SST806, increased the days to germination and reduced the coleoptile and radicle dry weights. The shoot dry weight was reduced by 75 and 39%, root dry weight by 73 and 37%, spike number of both by 50%, spike weight by 73 and 54%, grain number by 62 and 15%, grain weight per spike by 80 and 45%, and 1000 grain weight by 9 and 29% for 200 and 100 mM NaCl, respectively. Salicylic acid in combination with 100 mM and 200 mM NaCl increased the shoot, root, and yield attributes. Salicylic acid increased the grain protein content, especially at 200 mM NaCl, and the increase was higher in SST806 than PAN3497. The macro-mineral concentration was markedly increased by an increase of NaCl. This was further increased by salicylic acid treatment for both SST806 and PAN3497. Regarding micro-minerals, Na was increased more than the other minerals in both cultivars. Mn, Zn, Fe, and Cu were increased under 100 mM and 200 Mm of salt, and salicylic acid application increased these elements further in both cultivars. These results suggested that salicylic acid application improved the salt tolerance of these two bread wheat cultivars.
Three-Dimensional Boundary Element Strategy for Stress Sensitivity of Fractional-Order Thermo-Elastoplastic Ultrasonic Wave Propagation Problems of Anisotropic Fiber-Reinforced Polymer Composite Material
Authors: Fahmy Mohamed Abdelsabour
Published: July 2022

A new three-dimensional (3D) boundary element method (BEM) strategy was developed to solve fractional-order thermo-elastoplastic ultrasonic wave propagation problems based on the meshless moving least squares (MLS) method. The temperature problem domain was divided into a number of circular sub-domains. Each node was the center of the circular sub-domain surrounding it. The Laplace transform method was used to solve the temperature problem. A unit test function was used in the local weak-form formulation to generate the local boundary integral equations, and the inverse Laplace transformation method was used to find the transient temperature solutions. Then, the three-dimensional elastoplastic problems could be solved using the boundary element method (BEM). Initial stress and strain formulations are adopted, and their distributions are interpolated using boundary integral equations. The effects of the fractional-order parameter and anisotropy are investigated. The proposed method’s validity and performance are demonstrated for a two-dimensional problem with excellent agreement with other experimental and numerical results.
Effect of Sodium Carboxymethyl Cellulose on Water and Salt Transport Characteristics of Saline–Alkali Soil in Xinjiang, China
Authors: Zhang Jihong , Wang Quanjiu , Shan Yuyang , Guo Yi , Mu Weiyi , Wei Kai , Sun Yan
Published: July 2022

The scientific use of sodium carboxymethyl cellulose (CMC) to improve the production capacity of saline–alkali soil is critical to achieve green agriculture and sustainable land use. It serves as a foundation for the scientific use of CMC to clarify the water and salt transport characteristics of CMC-treated soil. In this study, a one-dimensional soil column infiltration experiment was carried out to investigate the effects of different CMC dosages (0, 0.2, 0.4, 0.6, and 0.8 g/kg) on the infiltration characteristics, infiltration model parameters, water and salt distribution, and salt leaching of saline–alkali soil in Xinjiang, China. The results showed that the final cumulative infiltration of CMC-treated soil increased by 8.63–20.72%, and the infiltration time to reach the preset wetting front depth increased by 1.02–3.96 times. The sorptivity (S) in the Philip infiltration model and comprehensive shape coefficient (α) in the algebraic infiltration model showed a trend of increasing first and then decreasing with CMC dosage, revealing a quadratic polynomial relationship. The algebraic model could accurately simulate the water content profile of CMC-treated soil. CMC enhanced the soil water holding capacity and salt leaching efficiency. The average soil water content, desalination rate, and leaching efficiency were increased by 5.18–15.54%, 21.17–57.15%, and 11.61–30.18%, respectively. The effect of water retention and salt inhibition on loamy sand was the best when the CMC dosage was 0.6 g/ kg. In conclusion, the results provide a theoretical basis for the rational application of CMC to improve saline–alkali soil in arid areas.