bluuebunny/crossref_metadata_2025 huggingface dataset:
Scheduling Appliances with GA, TLBO, FA, OSR and Their Hybrids Using Chance Constrained Optimization for Smart Homes
Authors: Nadeem Zunaira , Javaid Nadeem , Malik Asad Waqar , Iqbal Sohail
Published: April 2018

In this paper, we design a controller for home energy management based on following meta-heuristic algorithms: teaching learning-based optimization (TLBO), genetic algorithm (GA), firefly algorithm (FA) and optimal stopping rule (OSR) theory. The principal goal of designing this controller is to reduce the energy consumption of residential sectors while reducing consumer’s electricity bill and maximizing user comfort. Additionally, we propose three hybrid schemes OSR-GA, OSR-TLBO and OSR-FA, by combining the best features of existing algorithms. We have also optimized the desired parameters: peak to average ratio, energy consumption, cost, and user comfort (appliance waiting time) for 20, 50, 100 and 200 heterogeneous homes in two steps. In the first step, we obtain the optimal scheduling of home appliances implementing our aforementioned hybrid schemes for single and multiple homes while considering user preferences and threshold base policy. In the second step, we formulate our problem through chance constrained optimization. Simulation results show that proposed hybrid scheduling schemes outperformed for single and multiple homes and they shift the consumer load demand exceeding a predefined threshold to the hours where the electricity price is low thus following the threshold base policy. This helps to reduce electricity cost while considering the comfort of a user by minimizing delay and peak to average ratio. In addition, chance-constrained optimization is used to ensure the scheduling of appliances while considering the uncertainties of a load hence smoothing the load curtailment. The major focus is to keep the appliances power consumption within the power constraint, while keeping power consumption below a pre-defined acceptable level. Moreover, the feasible regions of appliances electricity consumption are calculated which show the relationship between cost and energy consumption and cost and waiting time.
Conditional Performance Evaluation: Using Wildfire Observations for Systematic Fire Simulator Development
Authors: Duff Thomas , Cawson Jane , Cirulis Brett , Nyman Petter , Sheridan Gary , Tolhurst Kevin
Published: April 2018

Faster than real-time wildland fire simulators are being increasingly adopted by land managers to provide decision support for tactical wildfire management and assist with strategic risk planning. These simulators are typically based on simple forward rate-of-spread algorithms that were predominantly developed using observations of experimental fires. Given their operational use, it is important that fire simulators be assessed in terms of their performance for their intended use; predicting the spatial progression of wildfires. However, the conditions under which wildfires occur cannot be easily replicated experimentally. We describe and demonstrate a method for use in model development, whereby a dataset comprised of wildfire case-studies is used for evaluating the predictive performance of fire simulators. Two different versions of the model PHOENIX RapidFire were assessed, one incorporating a novel algorithm that accounts fine-scale spatial variation in landscape dryness. Evaluation was done by comparing simulator predictions against contemporaneous observations of 9 different wildfires that occurred in Australia. Performance was quantified using the sum of the Area Difference Indices—a measure of prediction overlap calculated for each prediction/observation pair. The two versions of the model performed similarly, with the newer version being marginally (but not statistically significantly) better when outcomes were summarised across all fires. Despite this, it did not perform better in all cases, with three of the 9 fires better predicted using the original model. Wildfire evaluation datasets were demonstrated to provide valuable feedback for model development, however the limited availability of data means power is lacking for detailed comparisons. With increasingly extreme weather conditions resulting from climate change, conditions under which wildfires occur are likely to continue to extend well beyond those under which fire models algorithms were developed. Consequently, the adoption of improved methods for collecting and utilising wildfire data is critical to ensure fire simulators retain relevance.
Downed Coarse Woody Debris Dynamics in Ash (Fraxinus spp.) Stands Invaded by Emerald Ash Borer (Agrilus planipennis Fairmaire)
Authors: Perry Kayla , Herms Daniel , Klooster Wendy , Smith Annemarie , Hartzler Diane , Coyle David , Gandhi Kamal
Published: April 2018

Emerald ash borer (EAB; Agrilus planipennis Fairmaire) has had major ecological impacts in forests of eastern North America. In 2008 and 2012, we characterized dynamics of downed coarse woody debris (DCWD) in southeastern Michigan, USA near the epicenter of the invasion, where the mortality of white (Fraxinus americana L.), green (F. pennsylvanica Marshall), and black (F. nigra Marshall) ash exceeded 99% by 2009. Percentage of fallen dead ash trees and volume of ash DCWD on the forest floor increased by 76% and 53%, respectively, from 2008 to 2012. Ash and non-ash fell non-randomly to the east and southeast, conforming to prevailing winds. More ash fell by snapping along the bole than by uprooting. By 2012, however, only 31% of ash snags had fallen, indicating that DCWD will increase substantially, especially if it accelerates from the rate of 3.5% per year documented during the study period. Decay of ash DCWD increased over time, with most categorized as minimally decayed (decay classes 1 and 2) in 2008 and more decayed (decay classes 2 and 3) in 2012. As the range of EAB expands, similar patterns of DCWD dynamics are expected in response to extensive ash mortality.
Can Land Management Buffer Impacts of Climate Changes and Altered Fire Regimes on Ecosystems of the Southwestern United States?
Authors: Loehman Rachel , Flatley Will , Holsinger Lisa , Thode Andrea
Published: April 2018

Climate changes and associated shifts in ecosystems and fire regimes present enormous challenges for the management of landscapes in the Southwestern US. A central question is whether management strategies can maintain or promote desired ecological conditions under projected future climates. We modeled wildfire and forest responses to climate changes and management activities using two ecosystem process models: FireBGCv2, simulated for the Jemez Mountains, New Mexico, and LANDIS-II, simulated for the Kaibab Plateau, Arizona. We modeled contemporary and two future climates—“Warm-Dry” (CCSM4 RCP 4.5) and “Hot-Arid” (HadGEM2ES RCP 8.5)—and four levels of management including fire suppression alone, a current treatment strategy, and two intensified treatment strategies. We found that Hot-Arid future climate resulted in a fundamental, persistent reorganization of ecosystems in both study areas, including biomass reduction, compositional shifts, and altered forest structure. Climate changes increased the potential for high-severity fire in the Jemez study area, but did not impact fire regime characteristics in the Kaibab. Intensified management treatments somewhat reduced wildfire frequency and severity; however, management strategies did not prevent the reorganization of forest ecosystems in either landscape. Our results suggest that novel approaches may be required to manage future forests for desired conditions.
Elevated CO2 and O3 Levels Influence the Uptake and Leaf Concentration of Mineral N, P, K in Phyllostachys edulis (Carrière) J.Houz. and Oligostachyum lubricum (wen) King f.
Authors: Zhuang Minghao , Li Yingchun , Guo Ziwu , Li Yueqiao , Pan Wenting , Chen Shuanglin
Published: April 2018

Rising CO2 and O3 concentrations significantly affect plant growth and can alter nutrient cycles. However, the effects of elevated CO2 and O3 concentrations on the nutrient dynamics of bamboo species are not well understood. In this study, using open top chambers (OTCs), we examined the effects of elevated CO2 and O3 concentrations on leaf biomass and nutrient (N, P, and K) dynamics in two bamboo species, Phyllostachys edulis (Carrière) J.Houz. and Oligostachyum lubricum (wen) King f. Elevated O3 significantly decreased leaf biomass and nutrient uptake of both bamboo species, with the exception of no observed change in K uptake by O. lubricum. Elevated CO2 increased leaf biomass, N and K uptake of both bamboo species. Elevated CO2 and O3 simultaneously had no significant influence on leaf biomass of either species but decreased P and N uptake in P. edulis and O. lubricum, respectively, and increased K uptake in O. lubricum. The results indicate that elevated CO2 alleviated the damage caused by elevated O3 in the two bamboo species by altering the uptake of certain nutrients, which further highlights the potential interactive effects between the two gases on nutrient uptake. In addition, we found differential responses of nutrient dynamics in the two bamboo species to the two elevated gases, alone or in combination. These findings will facilitate the development of effective nutrient management strategies for sustainable management of P. edulis and O. lubricum under global change scenarios.
An Intelligent Content Prefix Classification Approach for Quality of Service Optimization in Information-Centric Networking
Authors: Safitri Cutifa , Yamada Yoshihide , Baharun Sabariah , Goudarzi Shidrokh , Ngoc Nguyen Quang , Yu Keping , Sato Takuro
Published: April 2018

This research proposes an intelligent classification framework for quality of service (QoS) performance improvement in information-centric networking (ICN). The proposal works towards keyword classification techniques to obtain the most valuable information via suitable content prefixes in ICN. In this study, we have achieved the intelligent function using Artificial Intelligence (AI) implementation. Particularly, to find the most suitable and promising intelligent approach for maintaining QoS matrices, we have evaluated various AI algorithms, including evolutionary algorithms (EA), swarm intelligence (SI), and machine learning (ML) by using the cost function to assess their classification performances. With the goal of enabling a complete ICN prefix classification solution, we also propose a hybrid implementation to optimize classification performances by integration of relevant AI algorithms. This hybrid mechanism searches for a final minimum structure to prevent the local optima from happening. By simulation, the evaluation results show that the proposal outperforms EA and ML in terms of network resource utilization and response delay for QoS performance optimization.
Impact of Rodenticides on the Coagulation Properties of Milk
Authors: Ibrahim Salam , Tse Tom
Published: April 2018

In this study, we investigated the impact of the rodenticides (strychnine, bromadiolone, and brodifacoum) on milk pH, rennet coagulation time (RCT), and coagulum strength. Sub-lethal amounts of strychnine and bromadiolone produced an unnaturally large change in milk pH, compared to brodifacoum and brodifacoum on milk coagulation properties. All three studied rodenticides significantly affected RCT and coagulum strength. The presence of sub-lethal amounts of each individual rodenticide increased RCT by an overall mean of 17% (p < 0.001). Rodenticide contamination decreased coagulum strength by an overall mean of 26% (p < 0.05). Our results suggest that such changes could be noticeable at the farm, thus, potentially averting the mixture of contaminated milk with the tanker supply, and preventing downstream distribution to consumers.
Searching for Axion-Like Particles with X-ray Polarimeters
Authors: Day Francesca , Krippendorf Sven
Published: April 2018

X-ray telescopes are an exceptional tool for searching for new fundamental physics. In particular, X-ray observations have already placed world-leading bounds on the interaction between photons and axion-like particles (ALPs). ALPs are hypothetical new ultra-light particles motivated by string theory models. They can also act as dark matter and dark energy, and provide a solution to the strong CP problem. In a background magnetic field, ALPs and photons may interconvert. This leads to energy dependent modulations in both the flux and polarisation of the spectra of point sources shining through large magnetic fields. The next generation of polarising X-ray telescopes will offer new detection possibilities for ALPs. Here we present techniques and projected bounds for searching for ALPs with X-ray polarimetry. We demonstrate that upcoming X-ray polarimetry missions have the potential to place world-leading bounds on ALPs.
Probabilistic Estimates of Ground Motion in the Los Angeles Basin from Scenario Earthquakes on the San Andreas Fault
Authors: Mourhatch Ramses , Krishnan Swaminathan
Published: April 2018

Kinematic source inversions of past earthquakes in the magnitude range of 6–8 are used to simulate 60 scenario earthquakes on the San Andreas fault. The unilateral rupture scenario earthquakes are hypothetically located at 6 locations spread out uniformly along the southern section of the fault, each associated with two hypocenters and rupture directions. Probabilities of occurrence over the next 30 years are assigned to each of these earthquakes by mapping the probabilities of 10,445 plausible earthquakes postulated for this section of the fault by the Uniform California Earthquake Rupture Forecast. Three-component broadband ground motion histories are computed at 636 sites in the greater Los Angeles metropolitan area by superposing short-period (0.2–2.0 s) empirical Green’s function synthetics on top of long-period (>2.0 s) spectral element synthetics. The earthquake probabilities and the computed ground motions are combined to develop probabilistic estimates of ground shaking in the region from San Andreas fault earthquakes over the next 30 years. The results could be useful in city planning, emergency management, and building code enhancement.
Decision Tree Approach to the Impact of Parents’ Oral Health on Dental Caries Experience in Children: A Cross-Sectional Study
Authors: Dima Shinechimeg , Wang Kung-Jeng , Chen Kun-Huang , Huang Yung-Kai , Chang Wei-Jen , Lee Sheng-Yang , Teng Nai-Chia
Published: April 2018

Decision tree (DT) analysis was applied in this cross-sectional study to investigate caries experience in children by using clinical and microbiological data obtained from parent–child pairs. Thirty pairs of parents and children were recruited from periodontal and pediatric dental clinics. All participants were clinically examined for caries and periodontitis by a calibrated examiner. Cariogenic and periodontopathic bacteria examinations were conducted. The Kendall rank correlation coefficient was used to measure the association between data variables obtained through clinical and microbiological examinations. A classificatory inductive decision tree was generated using the C4.5 algorithm with the top-down approach. The C4.5 DT analysis was applied to classify major influential factors for children dental caries experience. The DT identified parents’ periodontal health classification, decayed, missing, filled permanent teeth (DMFT) index, periodontopathic test (PerioCheck) result, and periodontal pocket depth as the classification factors for children caries experience. 13.3% of children were identified with a low decayed, missing, filled primary teeth (dmft) index (dmft < 3) whose parents had a periodontal pocket depth ≤3.7, PerioCheck score >1, DMFT index <13.5, and periodontal classification >2. The DT model for this study sample had an accuracy of 93.33%. Here, parental periodontal status and parents’ DMFT were the factors forming the DT for children’s caries experience.
Radon Levels in Indoor Environments of the University Hospital in Bari-Apulia Region Southern Italy
Authors: Vimercati Luigi , Fucilli Fulvio , Cavone Domenica , De Maria Luigi , Birtolo Francesco , Ferri Giovanni , Soleo Leonardo , Lovreglio Piero
Published: April 2018

Since 1988, the International Agency for Research on Cancer (IARC) has classified radon among the compounds for which there is scientific evidence of carcinogenicity for humans (group 1). The World Health Organization (WHO) recommends a reference radon level between 100 and 300 Bq/m3 for homes. The objective of this study is to measure the radon concentrations in 401 workplaces, different from the patient rooms, in 28 different buildings of the university hospital in Bari (Apulia region, Southern Italy) to evaluate the exposure of health care workers. Radon environmental sampling is performed over two consecutive six-month periods via the use of passive dosimeters of the CR-39 type. We find an average annual radon concentration expressed as median value of 48.0 Bq/m3 (range 6.5–388.0 Bq/m3) with a significant difference between the two six-month periods (median value: February/July 41.0 Bq/m3 vs. August/January 55.0 Bq/m3). An average concentration of radon lower than the WHO reference level (100 Bq/m3) is detected in 76.1% of monitored environments, while higher than 300 Bq/m3 only in the 0.9%. Most workplaces report radon concentrations within the WHO reference level, therefore, the risk to workers’ health deriving from occupational exposure to radon can be considered to be low. Nevertheless, the goal is to achieve near-zero exposures to protect workers’ health.
German Public Support for Tobacco Control Policy Measures: Results from the German Study on Tobacco Use (DEBRA), a Representative National Survey
Authors: Boeckmann Melanie , Kotz Daniel , Shahab Lion , Brown Jamie , Kastaun Sabrina
Published: April 2018

Smoking prevalence in Germany remains high at approximately 28%. We assessed public support for tobacco legislation and associations between level of support and smoking and socio-demographic characteristics. Data from 2087 people were collected as part of the German Study on Tobacco Use (“DEBRA”): a nationally representative, face-to-face household survey. Public support was measured on total ban of sale, raising the minimum age for sales, taxation of tobacco industry sales, research into e-cigarettes, and ban of smoking in cars when children are present. Associations were assessed with multivariate logistic regression. Over 50% of the German population support taxing industry profits (57.3%) and assessing e-cigarettes as an aid to quit smoking (55.5%). Over 40% support raising the legal age of sale (43.1%), and 22.9% support a total ban on tobacco sales. A smoking ban in cars when children are present was most popular (71.5%), even among current smokers (67.0%). There is public support for stricter tobacco control measures in Germany. A smoking ban in cars when children are present could be a feasible policy to implement.
Relationship between the Manner of Mobile Phone Use and Depression, Anxiety, and Stress in University Students
Authors: Višnjić Aleksandar , Veličković Vladica , Sokolović Dušan , Stanković Miodrag , Mijatović Kristijan , Stojanović Miodrag , Milošević Zoran , Radulović Olivera
Published: April 2018

Objectives: There is insufficient evidence regarding the potential risk of mobile phone use on mental health. Therefore, the aim of this research was to examine the relationship between mobile phone use and mental health by measuring the levels of depression, anxiety, and stress among university students in Serbia and Italy. Methods: This cross-sectional study was carried out at two distinguished universities in Serbia and Italy from March to May of the 2015/2016 academic year and included 785 students of both genders. The questionnaire was compiled and developed from different published sources regarding the manner and intensity of mobile phone use, along with the Depression Anxiety Stress Scale (DASS 42) for measuring psychological health. The statistical analysis of the data included the application of binary logistic regression and correlation tests. Results: Statistical analysis indicates that anxiety symptoms are somewhat more present in younger students (odds ratio (OR) = 0.86, 95% confidence interval (CI): 0.76–0.96), in those who send more text messages SMSs (OR = 1.15, 95% CI: 1.11–1.31), and in those who browse the internet less frequently (OR = 0.84, 95% CI: 0.73–0.95). Stress is more common in students who make fewer calls a day (OR = 0.79, 95% CI: 0.64–0.97), as well in those who spend more time talking on the mobile phone per day (OR = 1.28, 95% CI: 1.12–1.56). The strongest predictor of high stress levels was keeping the mobile phone less than 1 m away during sleeping (OR = 1.48, 95% CI: 1.12–2.08). Conclusions: The results indicated that the intensity and modality of mobile phone use could be a factor that can influence causal pathways leading to mental health problems in the university student population.
A Novel Pb-Resistant Bacillus subtilis Bacterium Isolate for Co-Biosorption of Hazardous Sb(III) and Pb(II): Thermodynamics and Application Strategy
Authors: Cai Yue , Li Xiaoping , Liu Dongying , Xu Changlin , Ai Yuwei , Sun Xuemeng , Zhang Meng , Gao Yu , Zhang Yuchao , Yang Tao , Wang Jingzhi , Wang Lijun , Li Xiaoyun , Yu Hongtao
Published: April 2018

The present work is the first to study co-biosorption of Pb(II) and Sb(III) by a novel bacterium and its application strategy. The biosorption characteristics of Pb(II) and Sb(III) ions from aqueous solution using B. subtilis were investigated. Optimum pH, biomass dosage, contact time and temperature were determined to be 5.00, 6.00 mg/L, 45 min and 35 °C, respectively. Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (D-R) models were applied to describe the biosorption isotherm of the metal ions by B. subtilis. Results showed that Langmuir model fitted the equilibrium data of Pb(II) better than others, while biosorption of Sb(III) obeyed the Freundlich model well. The biosorption capacity of B. subtilis biomass for Pb(II) and Sb(III) ions was found to be 17.34 ± 0.14 and 2.32 ± 0.30 mg/g, respectively. Kinetic data showed the biosorption process of Pb(II) and Sb(III) ions both followed the pseudo-second-order kinetic model, with R2 ranging from 0.974 to 0.999 for Pb(II) and from 0.967 to 0.979 for Sb(III). The calculated thermodynamic parameters, negative ∆G and positive ∆H and ∆S values, indicated the biosorption of Pb(II) and Sb(III) ions onto B. subtilis biomass in water was feasible, endothermic, and spontaneous. Bacterial bioleaching experiment revealed B. subtilis can increase the mobility of Pb(II) and Sb(III) in polluted soil when pH was close to 6 at low temperature. Consequently, B. subtilis, as a cheap and original bacterial material, could be a promising biomass to remove Pb or isolate Sb from industrial wastewater and to assist phytoremediation of Pb and Sb from weak acid or near neutral pH polluted soils at low temperature.
Understanding and Increasing Influenza Vaccination Acceptance: Insights from a 2016 National Survey of U.S. Adults
Authors: Nowak Glen , Cacciatore Michael , Len-Ríos María
Published: April 2018

Background: The percentage of adults in the U.S. getting seasonal influenza vaccination has not changed significantly since 2013 and remains far below the federal government’s 70% target. Objective: This study assessed and identified characteristics, experiences, and beliefs associated with influenza vaccination using a nationally representative survey of 1005 U.S. adults 19 years old and older. Methods: The sample was drawn from the National Opinion Research Center’s AmeriSpeak Panel, a probability-based panel designed to be representative of the U.S. household population. Results: Overall, 42.3% received an influenza vaccination in the past 12 months, with rates highest for non-Hispanic Whites and Blacks and those 65 years old and older. Hispanic respondents and those under 64 years old were much less likely to get an influenza vaccination. They were also less aware of the recommendation, less informed about influenza and the benefits of vaccination, and least confident in the vaccine. Conclusions: Increasing influenza vaccination coverage in the U.S. requires a greater focus on 19–64 year-olds, particularly those 50 to 64, Hispanics and continued focus on those with diabetes and asthma. Efforts need to increase awareness of influenza vaccination recommendations, foster a sense of being well informed about influenza vaccination benefits and the risks associated with non-vaccination, and increase confidence that there are meaningful benefits from receiving an influenza vaccination.
Analysis of Sociodemographic, Psychological, and Genetic Factors Contributing to Depressive symptoms in Pre-, Peri- and Postmenopausal Women
Authors: Grochans Elżbieta , Szkup Małgorzata , Kotwas Artur , Kopeć Jacek , Karakiewicz Beata , Jurczak Anna
Published: April 2018

Depressive symptoms that are faced by women in the pre-, peri-, and postmenopausal periods are determined by a wide array of sociodemographic, psychological, and biological variables. The aim of our study was to identify factors that contribute to depressive problems at this stage of life. The study included 815 healthy Polish women aged 45–60 years. The survey part was conducted using the Beck Depression Inventory (BDI), the State–Trait Anxiety Inventory (STAI), the Neuroticism–Extroversion–Openness Five Factor Inventory (NEO-FFI), and a self-developed questionnaire. Genetic analysis was also performed. Depressive symptoms were observed in 25.5% of participants. 70% of the women were postmenopausal. No statistically significant differences in the severity of depressive symptoms were demonstrated with regard to genetic variables (p > 0.05). Reproductive capacity (p < 0.001), employment (p < 0.001), and being married (p < 0.018) were found to reduce the incidence of depressive symptoms. The contribution of personality and anxiety as a trait to depressive symptoms varied. Conclusions: The factors predisposing pre-, peri-, and postmenopausal women to depressive symptoms include lower education, lack of a life partner, unemployment, high anxiety, and neurotic personality. No evidence was found for the contribution of genetic factors to depressive symptoms in the examined women.
Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry
Authors: Bockerstett Kevin , Wong Chun , Koehm Sherri , Ford Eric , DiPaolo Richard
Published: April 2018

The ability to analyze individual epithelial cells in the gastric mucosa would provide important insight into gastric disease, including chronic gastritis and progression to gastric cancer. However, the successful isolation of viable gastric epithelial cells (parietal cells, neck cells, chief cells, and foveolar cells) from gastric glands has been limited due to difficulties in tissue processing. Furthermore, analysis and interpretation of gastric epithelial cell flow cytometry data has been difficult due to the varying sizes and light scatter properties of the different epithelial cells, high levels of autofluorescence, and poor cell viability. These studies were designed to develop a reliable method for isolating viable single cells from the corpus of stomachs and to optimize analyses examining epithelial cells from healthy and diseased stomach tissue by flow cytometry. We performed a two stage enzymatic digestion in which collagenase released individual gastric glands from the stromal tissue of the corpus, followed by a Dispase II digestion that dispersed these glands into greater than 1 × 106 viable single cells per gastric corpus. Single cell suspensions were comprised of all major cell lineages found in the normal gastric glands. A method describing light scatter, size exclusion, doublet discrimination, viability staining, and fluorescently-conjugated antibodies and lectins was used to analyze individual epithelial cells and immune cells. This technique was capable of identifying parietal cells and revealed that gastric epithelial cells in the chronically inflamed mucosa significantly upregulated major histocompatibility complexes (MHC) I and II but not CD80 or CD86, which are costimulatory molecules involved in T cell activation. These studies describe a method for isolating viable single cells and a detailed description of flow cytometric analysis of cells from healthy and diseased stomachs. These studies begin to identify effects of chronic inflammation on individual gastric epithelial cells, a critical consideration for the study of gastric cancer.
d-Amino Acids Are Exuded by Arabidopsis thaliana Roots to the Rhizosphere
Authors: Hener Claudia , Hummel Sabine , Suarez Juan , Stahl Mark , Kolukisaoglu Üner
Published: April 2018

Proteinogenic l-amino acids (l-AAs) are essential in all kingdoms as building blocks of proteins. Their d-enantiomers are also known to fulfill important functions in microbes, fungi, and animals, but information about these molecules in plants is still sparse. Previously, it was shown that d-amino acids (d-AAs) are taken up and utilized by plants, but their ways to reduce excessive amounts of them still remained unclear. Analyses of plant d-AA content after d-Ala and d-Glu feeding opened the question if exudation of d-AAs into the rhizosphere takes place and plays a role in the reduction of d-AA content in plants. The exudation of d-Ala and d-Glu could be confirmed by amino acid analyses of growth media from plants treated with these d-AAs. Further tests revealed that other d-AAs were also secreted. Nevertheless, treatments with d-Ala and d-Glu showed that plants are still able to reduce their contents within the plant without exudation. Further exudation experiments with transport inhibitors revealed that d-AA root exudation is rather passive and comparable to the secretion of l-AAs. Altogether, these observations argued against a dominant role of exudation in the regulation of plant d-AA content, but may influence the composition of the rhizosphere.
Beneficial Effects of Bioactive Compounds in Mulberry Fruits against Cisplatin-Induced Nephrotoxicity
Authors: Lee Dahae , Yu Jae , Lee Seoung , Hwang Gwi , Kang Ki , Park Jae , Kim Hyun , Kim Ki , Yamabe Noriko
Published: April 2018

Mulberry, the fruit of white mulberry tree (Morus alba L., Moraceae), is commonly used in traditional Chinese medicines as a sedative, tonic, laxative, and emetic. In our continuing research of the bioactive metabolites from mulberry, chemical analysis of the fruits led to the isolation of five compounds, 1–5. The compounds were identified as butyl pyroglutamate (1), quercetin 3-O-β-d-glucoside (2), kaempferol 3-O-β-d-rutinoside (3), rutin (4), and 2-phenylethyl d-rutinoside (5) by spectroscopic data analysis, comparing their nuclear magnetic resonance (NMR) data with those in published literature, and liquid chromatography–mass spectrometry analysis. The isolated compounds 1–5 were evaluated for their effects on anticancer drug-induced side effects by cell-based assays. Compound 1 exerted the highest protective effect against cisplatin-induced kidney cell damage. This effect was found to be mediated through the attenuation of phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase, p38, mitogen-activated protein kinase, and caspase-3 in cisplatin-induced kidney cell damage.
Healing of Osteochondral Defects Implanted with Biomimetic Scaffolds of Poly(ε-Caprolactone)/Hydroxyapatite and Glycidyl-Methacrylate-Modified Hyaluronic Acid in a Minipig
Authors: Hsieh Yi-Ho , Shen Bo-Yuan , Wang Yao-Horng , Lin Bojain , Lee Hung-Maan , Hsieh Ming-Fa
Published: April 2018

Articular cartilage is a structure lack of vascular distribution. Once the cartilage is injured or diseased, it is unable to regenerate by itself. Surgical treatments do not effectively heal defects in articular cartilage. Tissue engineering is the most potential solution to this problem. In this study, methoxy poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) and hydroxyapatite at a weight ratio of 2:1 were mixed via fused deposition modeling (FDM) layer by layer to form a solid scaffold. The scaffolds were further infiltrated with glycidyl methacrylate hyaluronic acid loading with 10 ng/mL of Transforming Growth Factor-β1 and photo cross-linked on top of the scaffolds. An in vivo test was performed on the knees of Lanyu miniature pigs for a period of 12 months. The healing process of the osteochondral defects was followed by computer tomography (CT). The defect was fully covered with regenerated tissues in the control pig, while different tissues were grown in the defect of knee of the experimental pig. In the gross anatomy of the cross section, the scaffold remained in the subchondral location, while surface cartilage was regenerated. The cross section of the knees of both the control and experimental pigs were subjected to hematoxylin and eosin staining. The cartilage of the knee in the experimental pig was partially matured, e.g., few chondrocyte cells were enclosed in the lacunae. In the knee of the control pig, the defect was fully grown with fibrocartilage. In another in vivo experiment in a rabbit and a pig, the composite of the TGF-β1-loaded hydrogel and scaffolds was found to regenerate hyaline cartilage. However, scaffolds that remain in the subchondral lesion potentially delay the healing process. Therefore, the structural design of the scaffold should be reconsidered to match the regeneration process of both cartilage and subchondral bone.
An Organic Solvent-Tolerant Lipase with Both Hydrolytic and Synthetic Activities from the Oleaginous Fungus Mortierella echinosphaera
Authors: Kotogán Alexandra , Zambrano Carolina , Kecskeméti Anita , Varga Mónika , Szekeres András , Papp Tamás , Vágvölgyi Csaba , Takó Miklós
Published: April 2018

Lipase enzymes of the oleaginous fungal group Mortierella are rarely studied. However, considering that most commercial lipases are derived from filamentous fungal sources, their investigation can contribute to the cost-effective development of new biotechnological processes. Here, an extracellular lipase with a molecular mass of 30 kDa was isolated from Mortierella echinosphaera CBS 575.75 and characterized. The purified lipase exhibited an optimal p-nitrophenyl palmitate (pNPP)-hydrolyzing activity at 25 °C and pH 6.6–7.0 and proved to be highly stable at temperatures up to 40 °C and under broad pH conditions. The enzyme was active under low temperatures, retaining 32.5% of its activity at 10 °C, and was significantly stable in polar and non-polar organic solvents. The Km, Vmax, and kcat for pNPP were 0.336 mM, 30.4 μM/min, and 45.7 1/min for pNPP and 0.333 mM, 36.9 μM/min, and 55.6 1/min for pNP-decanoate, respectively. The pNPP hydrolysis was inhibited by Hg2+, N-bromosuccinimide, and sodium dodecyl sulfate, while ethylenediaminetetraacetic acid and metal ions, such as Ca2+, Mg2+, Na+, and K+ enhanced the activity. The purified lipase had non-regioselective activity and wide substrate specificity, showing a clear preference for medium-chained p-nitrophenyl esters. Besides its good transesterification activity, the enzyme appeared as a suitable biocatalyst to operate selective esterification reactions to long-chained alkyl esters. Adsorption to Accurel MP1000 improved the storage stability of the enzyme at 5 °C. The immobilized lipase displayed tolerance to a non-aqueous environment and was reusable for up to five cycles without significant loss in its synthetic and hydrolytic activities. These findings confirm the applicability of both the free and the immobilized enzyme preparations in future research.
The Effects of Long-Term, Low- and High-Dose Beta-Carotene Treatment in Zucker Diabetic Fatty Rats: The Role of HO-1
Authors: Csepanyi Evelin , Czompa Attila , Szabados-Furjesi Peter , Lekli Istvan , Balla Jozsef , Balla Gyorgy , Tosaki Arpad , Bak Istvan
Published: April 2018

Nowadays, there is a growing interest in compounds derived from plants as potential raw materials for drug development. One of the most studied compounds is beta-carotene (BC). Several clinical studies can be found investigating the cardiovascular effects of BC, however, all these results are controversial. There is an increasing body of evidence showing that besides the well-known antioxidant properties, under strong oxidative circumstances, BC could become prooxidant as well. In this study, we investigated the effects of long-term, low- and high-dose BC treatment in ischemic/reperfused (ISA/REP) hearts isolated from Zucker diabetic fatty (ZDF) rats. The animals were treated with various daily doses of BC for 4 weeks and then hearts were isolated and subjected to 30 min of global ischemia (ISA) followed by 120 min of reperfusion (REP). Blood glucose levels were measured before, after two weeks, and at the end of the treatment. In isolated hearts, the myocardial function was registered. At the end of the reperfusion period, the infarct size (IS) and heme oxygenase-1 (HO-1) expression were measured. The results showed that a low dose of BC treatment significantly improved postischemic recovery, which was reflected in a decreased IS. Interestingly, when BC was applied at high concentrations, the observed protective effects were lost. Although BC treatment increased HO-1 expression, we did not observe a better heart function and/or decreased IS in the high-dose-treated group. Glucose tolerance tests showed a concentration-independent decrease in blood glucose levels. Our results suggest that long-term, low-dose BC treatment could be effective in the treatment of type-2-diabetes and related cardiovascular diseases.
Allosteric Activation of GDP-Bound Ras Isoforms by Bisphenol Derivative Plasticisers
Authors: Schöpel Miriam , Shkura Oleksandr , Seidel Jana , Kock Klaus , Zhong Xueyin , Löffek Stefanie , Helfrich Iris , Bachmann Hagen , Scherkenbeck Jürgen , Herrmann Christian , Stoll Raphael
Published: April 2018

The protein family of small GTPases controls cellular processes by acting as a binary switch between an active and an inactive state. The most prominent family members are H-Ras, N-Ras, and K-Ras isoforms, which are highly related and frequently mutated in cancer. Bisphenols are widespread in modern life because of their industrial application as plasticisers. Bisphenol A (BPA) is the best-known member and has gained significant scientific as well as public attention as an endocrine disrupting chemical, a fact that eventually led to its replacement. However, compounds used to replace BPA still contain the molecular scaffold of bisphenols. BPA, BPAF, BPB, BPE, BPF, and an amine-substituted BPAF-derivate all interact with all GDP-bound Ras-Isoforms through binding to a common site on these proteins. NMR-, SOScat-, and GDI- assay-based data revealed a new bisphenol-induced, allosterically activated GDP-bound Ras conformation that define these plasticisers as Ras allosteric agonists.
17β-Hydroxysteroid Dehydrogenase Type 2 Expression Is Induced by Androgen Signaling in Endometrial Cancer
Authors: Hashimoto Chiaki , Miki Yasuhiro , Tanaka Sota , Takagi Kiyoshi , Fue Misaki , Doe Zhulanqiqige , Li Bin , Yaegashi Nobuo , Suzuki Takashi , Ito Kiyoshi
Published: April 2018

Endometrial cancer is one of the most common female pelvic cancers and has been considered an androgen-related malignancy. Several studies have demonstrated the anti-cell proliferative effect of androgen on endometrial cancer cells; however, the mechanisms of the anti-cancer effect of androgen remain largely unclear. 17β-hydroxysteroid dehydrogenase type 2 (17β-HSD2), which catalyzes the conversion of E2 to E1, is known to be upregulated by androgen treatment in breast cancer cells. In this study, we therefore focused on the role of androgen on estrogen dependence in endometrial cancer. Dihydrotestosterone (DHT) was found to induce 17β-HSD2 mRNA and protein expression in HEC-1B endometrial cancer cells. DHT could also inhibit cell proliferation of HEC-1B when induced by estradiol treatment. In 19 endometrioid endometrial adenocarcinoma (EEA) tissues, intratumoral DHT concentration was measured by liquid chromatography/electrospray tandem mass spectrometry and was found to be significantly correlated with 17β-HSD2 immunohistochemical status. We further examined the correlations between 17β-HSD2 immunoreactivity and clinicopathological parameters in 53 EEA tissues. 17β-HSD2 status was inversely associated with the histological grade, clinical stage, and cell proliferation marker Ki-67, and positively correlated with progesterone receptor expression. 17β-HSD2 status tended to be positively associated with androgen receptor status. In 53 EEA cases, the 17β-HSD2-positive group tended to have better prognosis than that for the negative group with respect to progression-free survival and endometrial cancer-specific survival. These findings suggest that androgen suppresses the estrogen dependence of endometrial cancer through the induction of 17β-HSD2 in endometrial cancer.
CD147 Promotes CXCL1 Expression and Modulates Liver Fibrogenesis
Authors: Shi Wen-Pu , Ju Di , Li Hao , Yuan Lin , Cui Jian , Luo Dan , Chen Zhi-Nan , Bian Huijie
Published: April 2018

Activated hepatic stellate cells (HSCs) release pro-inflammatory and pro-fibrogenic factors. CXC chemokine-ligand-1 (CXCL1) is expressed on HSCs. We previously found that the CD147 is overexpressed in activated HSCs. In this study, we showed an important role of CD147 in promoting liver fibrosis by activating HSCs and upregulating expression of chemokines. Specifically, we found that CD147 specific deletion in HSCs mice alleviated CCl4-induced liver fibrosis and inhibited HSCs activation. Overexpression of CD147 upregulated the secretion of CXCL1. Meanwhile, CXCL1 promoted HSCs activation through autocrine. Treating with PI3K/AKT inhibitor could effectively suppress CD147-induced CXCL1 expression. Taken together, these findings suggest that CD147 regulates CXCL1 release in HSCs by PI3K/AKT signaling. Inhibition of CD147 attenuates CCl4-induced liver fibrosis and inflammation. Therefore, administration of targeting CD147 could be a promising therapeutic strategy in liver fibrosis.
Diagnostic Criteria and Clinical Outcomes in Sarcopenia Research: A Literature Review
Authors: Han Alex , Bokshan Steven , Marcaccio Stephen , DePasse J. , Daniels Alan
Published: April 2018

By the sixth decade of life, nearly one quarter of the population has substantial muscle atrophy, or sarcopenia. Despite the creation of a standardized definition of sarcopenia by the European Working Group on Sarcopenia in Older People, variability may exist in the diagnostic criteria utilized for clinical sarcopenia research. The primary objectives of this review were to characterize diagnostic criteria used for measurement of sarcopenia in original studies, and to describe associations between sarcopenia and important clinical outcomes. We performed a literature review of the term “sarcopenia” in PubMed. Inclusion criteria were English language, original data, a clear and specific definition for diagnosing sarcopenia, and the analysis of sarcopenia’s effect on a clinical outcome. A total of 283 studies met inclusion criteria. More than half of the included sarcopenia investigations were level IV studies (54.1%), while 43.1% provided level II evidence. Under one third (27.6%) of studies examined sarcopenia with regard to surgical outcomes. In terms of diagnostic criteria for sarcopenia, 264 (93.3%) studies used measures of skeletal muscle mass, with dual energy X-ray absorptiometry (DEXA) being the most common modality (43.6%). Sarcopenia was found to be a consistent predictor of chronic disease progression, all-cause mortality, poorer functional outcomes, and postoperative complications. In conclusion, there is substantial evidence that sarcopenia impacts both medical and surgical outcomes. However, current research has utilized heterogeneous diagnostic criteria for sarcopenia. Further efforts to standardize the modalities used to diagnose sarcopenia in clinical research and practice will help strengthen our ability to study this important phenomenon.
The Strengths of Wisdom Provide Unique Contributions to Improved Leadership, Sustainability, Inequality, Gross National Happiness, and Civic Discourse in the Face of Contemporary World Problems
Authors: Grossmann Igor , Brienza Justin P.
Published: April 2018

We present evidence for the strengths of the intellectual virtues that philosophers and behavioral scientists characterize as key cognitive elements of wisdom. Wisdom has been of centuries-long interest for philosophical scholarship, but relative to intelligence largely neglected in public discourse on educational science, public policy, and societal well-being. Wise reasoning characteristics include intellectual humility, recognition of uncertainty, consideration of diverse viewpoints, and an attempt to integrate these viewpoints. Emerging scholarship on these features of wisdom suggest that they uniquely contribute to societal well-being, improve leadership, shed light on societal inequality, promote cooperation in Public Goods Games and reduce political polarization and intergroup-hostility. We review empirical evidence about macro-cultural, ecological, situational, and person-level processes facilitating and inhibiting wisdom in daily life. Based on this evidence, we speculate about ways to foster wisdom in education, organizations, and institutions.
Peridynamic Analysis of Marine Composites under Shock Loads by Considering Thermomechanical Coupling Effects
Authors: Gao Yan , Oterkus Selda
Published: April 2018

Nowadays, composite materials have been increasingly used in marine structures because of their high performance properties. During their service time, they may be exposed to extreme loading conditions such as underwater explosions. Temperature changes induced by pure mechanical shock loadings cannot to be neglected especially when smart composite materials are employed for condition monitoring of critical systems in a marine structure. Considering this fact, both the thermal loading effect on deformation and the deformation effect on temperature need to be taken into consideration. Consequently, an analysis conducted in a fully coupled thermomechanical manner is necessary. Peridynamics is a newly proposed non-local theory which can predict failures without extra assumptions. Therefore, a fully coupled thermomechanical peridynamic model is developed for laminated composites materials. In this study, numerical analysis of a 13 ply laminated composite subjected to an underwater explosion is conducted by using the developed model. The pressure shocks generated by the underwater explosion are applied on the top surface of the laminate for uniform and non-uniform load distributions. The damage is predicted and compared with existing experimental results. The simulation results obtained from uncoupled case are also provided for comparison. Thus the coupling term effects on crack propagation paths are investigated. Furthermore, the corresponding temperature distributions are also investigated.