bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergistic Effect of Omega-3 Fatty Acids and Oral-Hypoglycemic Drug on Lipid Normalization through Modulation of Hepatic Gene Expression in High Fat Diet with Low Streptozotocin-Induced Diabetic Rats
Authors: Khadke Suresh , Mandave Pallavi , Kuvalekar Aniket , Pandit Vijaya , Karandikar Manjiri , Mantri Nitin
Published: November 2020

Type 2 diabetes mellitus, which an outcome of impaired insulin action and its secretion, is concomitantly associated with lipid abnormalities. The study was designed to evaluate the combinational effect of omega-3 fatty acids (flax and fish oil) and glibenclamide on abnormal lipid profiles, increased blood glucose, and impaired liver and kidney functions in a high fat diet with low streptozotocin (STZ)-induced diabetic rats, including its probable mechanism of action. The male Wistar rats (n = 48) were distributed into eight groups. All animal groups except the healthy received a high fat diet (HFD) for 90 days. Further, diabetes was developed by low dose STZ (35 mg/kg). Diabetic animals received, omega-3 fatty acids (500 mg/kg), along with glibenclamide (0.25 mg/kg). Both flax and fish oil intervention decreased (p ≤ 0.001) serum triglycerides and very low density lipoprotein and elevated (p ≤ 0.001) high density lipoprotein levels in diabetic rats. Total cholesterol and low-density lipoprotein level was decreased (p ≤ 0.001) in fish oil-treated rats. However, it remained unaffected in the flax oil treatment group. Both flax and fish oil intervention downregulate the expression of fatty acid metabolism genes, transcription factors (sterol regulatory element-binding proteins-1c and nuclear factor-κβ), and their regulatory genes i.e., acetyl-coA carboxylase alpha, fatty acid synthase, and tumor necrosis factors-α. The peroxisome proliferator-activated receptor gamma gene expression was upregulated (p ≤ 0.001) in the fish oil treatment group. Whereas, carnitine palmitoyltransferase 1 and fatty acid binding protein gene expression were upregulated (p ≤ 0.001) in both flax and fish oil intervention group.
The Effect of Acute Caffeine Ingestion on Cognitive Dual Task Performance during Assessment of Static and Dynamic Balance in Older Adults
Authors: Tallis Jason , Bradford Chelsea , Duncan Michael , Leddington-Wright Sheila , Higgins Matthew , Hill Matthew
Published: November 2020

The present work aimed to evaluate the effect of 3 mg·kg−1 caffeine consumption on the standing and dynamic balance performance of older adults and sought to establish if caffeine ingestion can modulate the influence of a cognitive dual task on balance performance. Twelve apparently healthy participants (8 females) aged >65 years (72 ± 3.7 years) completed the study. Bipedal postural sway, four square step test, timed up and go, Y-balance (anterior reach only) and force-time characteristics of sit-to-stand performance were used to assess standing and dynamic balance. Attention and working memory were assessed using a serial 3s and 7s subtraction task during seated rest and completion of the bipedal standing assessment and Y-balance test. This battery of assessments was completed on two separate occasions, once following the consumption of a non-ergogenic placebo and again following the consumption of 3 mg·kg−1 caffeine. The administration of treatments was randomised, counterbalanced and double-blind. Caffeine reduced performance in the bipedal standing balance assessments, evidenced by an increase in COPML, COPPath, COPVelocity. Performance during the dynamic balance tests was unaffected, other than rate of force development during the sit-to-stand, which was improved following caffeine ingestion. The introduction of a cognitive dual task had either limited effects, or improved facets of bipedal standing balance, whilst performance during the dynamic balance task was significantly reduced. In both balance assessments, there was evidence for a reduction in the performance of the cognitive task when both the balance and cognitive tests were performed simultaneously, with this effect not modulated by caffeine consumption. These findings refute the idea that caffeine ingestion may have positive effects on balance performance. However, despite a caffeine-induced reduction in bipedal standing balance, it is unlikely that caffeine ingestion would exacerbate fall risk given the limited effects in the dynamic balance tests. Future work should establish if these effects are generalisable to older frail participants and if caffeine can modulate the detrimental effects of an acute exercise bout on balance performance.
Phenethyl Isothiocyanate Protects against High Fat/Cholesterol Diet-Induced Obesity and Atherosclerosis in C57BL/6 Mice
Authors: Gwon Min-Hee , Im Young-Sun , Seo A-Reum , Kim Kyoung Yun , Moon Ha-Rin , Yun Jung-Mi
Published: November 2020

This study concerns obesity-related atherosclerosis, hyperlipidemia, and chronic inflammation. We studied the anti-obesity and anti-atherosclerosis effects of phenethyl isothiocyanate (PEITC) and explored their underlying mechanisms. We established an animal model of high fat/cholesterol-induced obesity in C57BL/6 mice fed for 13 weeks. We divided the mice into five groups: control (CON), high fat/cholesterol (HFCD), HFCD with 3 mg/kg/day gallic acid (HFCD + G), and HFCD with PEITC (30 and 75 mg/kg/day; HFCD + P30 and P75). The body weight, total cholesterol, and triglyceride were significantly lower in the HFCD + P75 group than in the HFCD group. Hepatic lipid accumulation and atherosclerotic plaque formation in the aorta were significantly lower in both HFCD + PEITC groups than in the HFCD group, as revealed by hematoxylin and eosin (H&E) staining. To elucidate the mechanism, we identified the expression of genes related to inflammation, reverse cholesterol transport, and lipid accumulation pathway in the liver. The expression levels of peroxisome proliferator activated receptor gamma (PPARγ), liver-X-receptor α (LXR-α), and ATP binding cassette subfamily A member 1 (ABCA1) were increased, while those of scavenger receptor A (SR-A1), cluster of differentiation 36 (CD36), and nuclear factor-kappa B (NF-κB) were decreased in the HFCD + P75 group compared with those in the HFCD group. Moreover, PEITC modulated H3K9 and H3K27 acetylation, H3K4 dimethylation, and H3K27 di-/trimethylation in the HFCD + P75 group. We, therefore, suggest that supplementation with PEITC may be a potential candidate for the treatment and prevention of atherosclerosis and obesity.
Molecular Characterization of Small Ruminant Lentiviruses of Subtype A5 Detected in Naturally Infected but Clinically Healthy Goats of Carpathian Breed
Authors: Olech Monika , Kuźmak Jacek
Published: November 2020

Small ruminant lentiviruses (SRLVs) are widespread in sheep and goats in Poland, and several subtypes were identified and molecularly characterized up to date. This is the first study that characterizes the molecular properties of A5 strains of SRLV detected in naturally infected, but clinically healthy, Carpathian goats. Segments from three genomic regions (gag, env, and LTR) were analyzed. Genetic distance, pairwise comparison, and phylogenetic analysis revealed that Polish SRLV A5 sequences are closely related to the Swiss and German A5 sequences suggesting a common origin. The epidemiological linkage was identified particularly between the small ruminants of Germany and Poland. Amino acid sequences of immunodominant regions in CA protein were well-conserved within analyzed strains; however, they showed some remarkable changes like substitution (D) to (E), at position 90 in Major Homology Region (MHR) and (T) to (S), at position 141 in epitope 3. In contrast, aa sequences of surface glycoprotein exhibited the highest variability confirming type-specific variation in SU5 epitope. Two deletions in the U3 region of A5 strains were noted: One (8 nt) located near the 5′ end of the U3 region and the other (29 nt) located in the central region of U3. Additionally, all A5 strains had specific deletion (10 nt) in the R region. Furthermore, we did not find a correlation between copies of the CAAAT motif and clinical manifestation in infected animals. These data showed some remarkable features in the viral genome of A5 strains, which may be related to the attenuated phenotype in vivo, characterized by the lack of any clinical signs in infected goats. Certainly, more studies are required to support the hypothesis that these A5 viruses are of low pathogenicity for goats. We want to focus our future studies on the analysis of the whole genomes of these isolates and their biological properties, as well as on clinicopathological studies of goats infected by A5 SRLV, aiming to clarify the pathogenic potential of these viruses.
Repurposing of Plasminogen: An Orphan Medicinal Product Suitable for SARS-CoV-2 Inhalable Therapeutics
Authors: Piras Anna Maria , Zambito Ylenia , Lugli Maurizio , Ferro Baldassare , Roncucci Paolo , Mori Filippo , Salvatore Alfonso , Ascione Ester , Bellini Marta , Crea Roberto
Published: November 2020

The SARS-CoV-2 infection is associated with pulmonary coagulopathy, which determines the deposition of fibrin in the air spaces and lung parenchyma. The resulting lung lesions compromise patient pulmonary function and increase mortality, or end in permanent lung damage for those who have recovered from the COVID-19 disease. Therefore, local pulmonary fibrinolysis can be efficacious in degrading pre-existing fibrin clots and reducing the conversion of lung lesions into lasting scars. Plasminogen is considered a key player in fibrinolysis processes, and in view of a bench-to-bedside translation, we focused on the aerosolization of an orphan medicinal product (OMP) for ligneous conjunctivitis: human plasminogen (PLG-OMP) eye drops. As such, the sterile and preservative-free solution guarantees the pharmaceutical quality of GMP production and meets the Ph. Eur. requirements of liquid preparations for nebulization. PLG-OMP aerosolization was evaluated both from technological and stability viewpoints, after being submitted to either jet or ultrasonic nebulization. Jet nebulization resulted in a more efficient delivery of an aerosol suitable for pulmonary deposition. The biochemical investigation highlighted substantial protein integrity maintenance with the percentage of native plasminogen band > 90%, in accordance with the quality specifications of PLG-OMP. In a coherent way, the specific activity of plasminogen is maintained within the range 4.8–5.6 IU/mg (PLG-OMP pre-nebulization: 5.0 IU/mg). This is the first study that focuses on the technological and biochemical aspects of aerosolized plasminogen, which could affect both treatment efficacy and clinical dosage delivery. Increasing evidence for the need of local fibrinolytic therapy could merge with the availability of PLG-OMP as an easy handling solution, readily aerosolizable for a fast translation into an extended clinical efficacy assessment in COVID-19 patients.
Monoclonal Antibodies: Leading Actors in the Relapsed/Refractory Multiple Myeloma Treatment
Authors: Morè Sonia , Petrucci Maria , Corvatta Laura , Fazio Francesca , Offidani Massimo , Olivieri Attilio
Published: November 2020

Multiple myeloma is a complex hematologic malignancy, and despite a survival improvement related to the growing number of available therapeutic options since 2000s, it remains an incurable disease with most patients experiencing relapse. However, therapeutic options for this disease are constantly evolving and immunotherapy is becoming the mainstay of the therapeutic armamentarium of Multiple Myeloma (MM), starting with monoclonal antibodies (MoAbs) as elotuzumab, daratumumab and isatuximab. Elotuzumab, the first in class targeting SLAMF7, in combination with lenalidomide and dexamethasone and daratumumab, directed against CD38, in combination with Rd and with bortezomib and dexamethasone (Vd), have been approved for the treatment of relapsed/refractory MM (RRMM) after they demonstrated excellent efficacy. More recently, another anti-CD38 MoAb named isatuximab was approved by FDA in combination with pomalidomide-dexamethasone (Pd) in the same setting. Many phase II and III trials with regimens containing these MoAbs are ongoing, and when available, preliminary data are very encouraging. In this review we will describe the results of major clinical studies that have been conducted with elotuzumab, daratumumab and isatuximab in RRMM, focusing on phase III trials. Moreover, we will summarized the emerging MoAbs-based combinations in the RRMM landscape.
Where Does Metformin Stand in Modern Day Management of Type 2 Diabetes?
Authors: Ahmad Ehtasham , Sargeant Jack , Zaccardi Francesco , Khunti Kamlesh , Webb David , Davies Melanie
Published: November 2020

Metformin is the most commonly used glucose-lowering therapy (GLT) worldwide and remains the first-line therapy for newly diagnosed individuals with type 2 diabetes (T2D) in management algorithms and guidelines after the UK Prospective Diabetes Study (UKPDS) showed cardiovascular mortality benefits in the overweight population using metformin. However, the improved Major Adverse Cardiovascular Events (MACE) realised in some of the recent large cardiovascular outcomes trials (CVOTs) using sodium-glucose co-transporter 2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor agonists (GLP-1RA) have challenged metformin’s position as a first-line agent in the management of T2D. Many experts now advocate revising the existing treatment algorithms to target atherosclerotic cardiovascular disease (ASCVD) and improving glycaemic control as a secondary aim. In this review article, we will revisit the major cardiovascular outcome data for metformin and include a critique of the UKPDS data. We then review additional factors that might be pertinent to metformin’s status as a first-line agent and finally answer key questions when considering metformin’s role in the modern-day management of T2D.
Safety and Photoprotective Efficacy of a Sunscreen System Based on Grape Pomace (Vitis vinifera L.) Phenolics from Winemaking
Authors: Hübner Alexandra A. , Sarruf Fernanda D. , Oliveira Camila A. , Neto Alberto V. , Fischer Dominique C. H. , Kato Edna T. M. , Lourenço Felipe R. , Baby André Rolim , Bacchi Elfriede M.
Published: November 2020

In winemaking, a large amount of grape pomace is produced that is rich in polyphenolics and highly beneficial for human health, as phenols are useful for skin ultraviolet (UV) protection. In this investigation, we evaluated the safety and clinical efficacy of a sunscreen system containing a grape pomace extract from Vitis vinifera L. as a bioactive ingredient. The recovery of phenolics in the waste was performed by percolation. Nine emulsions were developed using a factorial design and two were evaluated clinically: Formulation E, containing only UV filters (butylmethoxydibenzoyl methane, ethylhexyl methoxycinnamate and ethylhexyl dimethyl PABA), and F, with the extract at 10.0% w/w + UV filters. The antioxidant activity was determined by the DPPH assay and the in vitro efficacy was established by sun protection factor (SPF) measurements (Labsphere UV-2000S). Clinical tests were performed to determine safety (human repeated insult patch test) and to confirm efficacy (photoprotective effectiveness in participants). The results showed a synergistic effect between the sunscreen system and the extract on UVB protection and antioxidant activity. Both samples were considered safe. Formulation F was 20.59% more efficient in protecting skin against UVB radiation, taking approximately 21% more time to induce erythema compared to the extract-free sample.
Design and Characterization of Sodium Alginate and Poly(vinyl) Alcohol Hydrogels for Enhanced Skin Delivery of Quercetin
Authors: Esposito Ludovico , Barbosa Ana Isabel , Moniz Tânia , Costa Lima Sofia , Costa Paulo , Celia Christian , Reis Salette
Published: November 2020

Nature has led to the discovery of biopolymers with noteworthy pharmaceutical applications. Blended biopolymers have demonstrated promising characteristics when compared with their individual counterparts. Sodium alginate (SA) is a marine polymer that has demonstrated the ability to form hydrogels, an interesting property for the development of cutaneous formulations. Predicting the good performance of blended biopolymers, a novel series of hybrid hydrogels based on SA and poly(vinyl) alcohol (PVA) were prepared. Quercetin, a natural polyphenolic flavonoid commonly found in fruits and vegetables, is widely known for its strong anti-inflammatory and antioxidant activity, thus with potential applications against melanoma, dermatitis, psoriasis, and skin ageing. Here, hydrogels were produced at different ratios of SA and PVA. The surface morphology, structure, interaction of polymers, the capacity to absorb water and the entrapment efficiency of quercetin were evaluated for the blended hydrogels. Targeting the cutaneous application of the formulations, the rheological properties of all unloaded and quercetin-loaded hydrogels revealed pseudoplastic behavior, evidence of non-thixotropy, good resistance to deformation, and profile maintenance with temperatures ranging from 20 °C up to 40 °C. The incorporation of quercetin in the hydrogel retained its antioxidant activity, confirmed by radical scavenging assays (ABTS and DPPH). The permeability of quercetin through the skin showed different penetration/permeation profiles according to the hydrogel’s blend. This behavior will allow the selection of SA-PVA at 2/1 ratio for a local and prolonged skin effect, making the use of these hydrogels a good solution to consider for the treatment of skin ageing and inflammation.
Topical Delivery of Carvedilol Loaded Nano-Transfersomes for Skin Cancer Chemoprevention
Authors: Chen Mengbing , Shamim Md Abdullah , Shahid Ayaz , Yeung Steven , Andresen Bradley T. , Wang Jeffrey , Nekkanti Vijaykumar , Meyskens Frank L. , Kelly Kristen M. , Huang Ying
Published: November 2020

The β-blocker carvedilol has been shown to prevent skin carcinogenesis in vitro and in vivo. Since systemic absorption of the β-blocker may cause cardiovascular disturbance, we developed a carvedilol loaded transfersome for skin-targeted delivery. Transfersomes were prepared using phospholipids and surfactants at various ratios and characterized. One formulation (F18) selected for further analysis was composed of carvedilol, soy phosphatidylcholine, and Tween-80 at a ratio of 1:3:0.5, which had a particle size of 115.6 ± 8.7 nm, a zeta potential of 11.34 ± 0.67 mV, and an encapsulation efficiency of 93.7 ± 5.1%. F18 inhibited EGF-induced neoplastic transformation of mouse epidermal JB6 P+ cells at non-toxic concentrations, while only high concentrations induced cytotoxicity in JB6 P+ and human keratinocytes HaCaT. Compared to the free drug, F18 released through the dialysis membrane and permeated through the porcine ear skin at a slower rate, but similarly depositing the drug in the epidermis and dermis of the skin. Consistently, surface application of F18 on reconstructed full-thickness human skin showed slower drug permeation, while it suppressed ultraviolet-induced DNA damage, inflammatory gene expression, and apoptosis. These data indicate that transfersome is a promising topical delivery system of carvedilol for preventing ultraviolet-induced skin damage and carcinogenesis.
Characterization of Simultaneous Evolution of Size and Composition Distributions Using Generalized Aggregation Population Balance Equation
Authors: Singh Mehakpreet , Kumar Ashish , Shirazian Saeed , Ranade Vivek , Walker Gavin
Published: November 2020

The application of multi-dimensional population balance equations (PBEs) for the simulation of granulation processes is recommended due to the multi-component system. Irrespective of the application area, numerical scheme selection for solving multi-dimensional PBEs is driven by the accuracy in (size) number density prediction alone. However, mixing the components, i.e., the particles (excipients and API) and the binding liquid, plays a crucial role in predicting the granule compositional distribution during the pharmaceutical granulation. A numerical scheme should, therefore, be able to predict this accurately. Here, we compare the cell average technique (CAT) and finite volume scheme (FVS) in terms of their accuracy and applicability in predicting the mixing state. To quantify the degree of mixing in the system, the sum-square χ2 parameter is studied to observe the deviation in the amount binder from its average. It has been illustrated that the accurate prediction of integral moments computed by the FVS leads to an inaccurate prediction of the χ2 parameter for a bicomponent population balance equation. Moreover, the cell average technique (CAT) predicts the moments with moderate accuracy; however, it computes the mixing of components χ2 parameter with higher precision than the finite volume scheme. The numerical testing is performed for some benchmarking kernels corresponding to which the analytical solutions are available in the literature. It will be also shown that both numerical methods equally well predict the average size of the particles formed in the system; however, the finite volume scheme takes less time to compute these results.
Morphostatic Speciation within the Dagger Nematode Xiphinema hispanum-Complex Species (Nematoda: Longidoridae)
Authors: Archidona-Yuste Antonio , Cai Ruihang , Cantalapiedra-Navarrete Carolina , Carreira José A. , Rey Ana , Viñegla Benjamín , Liébanas Gracia , Palomares-Rius Juan E. , Castillo Pablo
Published: November 2020

Dagger nematodes of the genus Xiphinema include a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of Xiphinema species is critical for launching appropriate control measures. We deciphered the cryptic diversity of the Xiphinema hispanum-species complex applying integrative taxonomical approaches that allowed us to verify a paradigmatic example of the morphostatic speciation and the description of a new species, Xiphinema malaka sp. nov. Detailed morphological, morphometrical, multivariate and genetic studies were carried out, and mitochondrial and nuclear haploweb analyses were used for species delimitation of this group. The new species belongs to morphospecies Group 5 from the Xiphinema nonamericanum-group species. D2-D3, ITS1, partial 18S, and partial coxI regions were used for inferring the phylogenetic relationships of X. malaka sp. nov. with other species within the genus Xiphinema. Molecular analyses showed a clear species differentiation not paralleled in morphology and morphometry, reflecting a clear morphostatic speciation. These results support the hypothesis that the biodiversity of dagger nematodes in southern Europe is greater than previously assumed.
Rootstock-Scion Interaction Affects the Composition and Pathogen Inhibitory Activity of Tobacco (Nicotiana tabacum L.) Root Exudates
Authors: Zhang Cheng-Sheng , Zheng Yanfen , Peng Lijuan , Cao Jianmin
Published: November 2020

The composition and allelopathy to Phytophthora nicotianae (the causal agent of tobacco black shank disease) of root exudates from a resistant tobacco (Nicotiana tabacum L.) cultivar Gexin 3, a susceptible cultivar Xiaohuangjin 1025 and their reciprocal grafts were investigated. Grafting with disease-resistant rootstock could improve resistance to black shank; this is closely related to the allelopathy of root exudates. The root exudates from the resistant cultivar inhibited the growth of P. nicotianae, while those from the susceptible cultivar promoted the growth; the grafting varieties had intermediate properties. The root exudate composition differed among cultivars. Gexin 3 was rich in esters and fatty acids, while Xiaohuangjin 1025 contained more hydrocarbons and phenolic acids. The composition of root exudates of grafted cultivars as well as their allelopathy to P. nicotianae were altered, and tended to be close to the composition of cultivar used as rootstock. Eugenol, 4-tert-butylphenol, mono (2-ethylhexyl) phthalate, 4-hydroxybenzoic acid, 2,6-di-tert-butylphenol, dipropyl phthalate, and methyl myristate were identified as the main compounds contributing to inhibitory properties of root exudates. Sorbitol was suggested to play a role in disease induction. Overall, rootstock–scion interaction affected the composition of tobacco root exudates, which may be attributed to the different disease resistance among grafted plants, rootstock and scion.
Extraction and Study of the Essential Oil of Copal (Dacryodes peruviana), an Amazonian Fruit with the Highest Yield Worldwide
Authors: Valarezo Eduardo , Ojeda-Riascos Santiago , Cartuche Luis , Andrade-González Nathaly , González-Sánchez Inés , Meneses Miguel Angel
Published: November 2020

Essential oils are highly demanded substances worldwide. They can be used without modification due to their different chemical and biological properties or as natural sources of chemical compounds. The limit in the use of these metabolites is their low yield. In the present investigation, the essential oil of fruits from Dacryodes peruviana collected in the Ecuadorian Amazon was extracted and studied. The essential oil was released from the plant matrix and isolated by hydrodistillation. The yields obtained were 4.8 ± 0.2% and 11.3 ± 0.2% for fresh and dried fruits, respectively, one of the highest yields on record to date. Twenty-five chemical compounds were identified by GC/MS and GC/FID techniques. The principal constituent was α-phellandrene, with 50.32 ± 3.32%. The antimicrobial activity of the oil was assayed against five Gram negative bacteria, two Gram positive bacteria and two fungi. The essential oil exerted a moderate activity against Staphylococcus aureus. The repellent activity of the oil was assayed against mosquitoes (Diptera: Culicidae); the samples with 3%, 2% and 1% essential oil were class 4, and the sample with 0.5% showed to be class 3. The essential oil showed a weak antioxidant activity through the DPPH and ABTS methods.
Identification of Novel Loci and Candidate Genes for Cucumber Downy Mildew Resistance Using GWAS
Authors: Liu Xiaoping , Lu Hongwei , Liu Panna , Miao Han , Bai Yuling , Gu Xingfang , Zhang Shengping
Published: November 2020

Downy mildew (DM) is one of the most serious diseases in cucumber. Multiple quantitative trait loci (QTLs) for DM resistance have been detected in a limited number of cucumber accessions. In this study we applied genome-wide association analysis (GWAS) to detected genetic loci for DM resistance in a core germplasm (CG) of cucumber lines that represent diverse origins and ecotypes. Phenotypic data on responses to DM infection were collected in four field trials across three years, 2014, 2015, and 2016. With the resequencing data of these CG lines, GWAS for DM resistance was performed and detected 18 loci that were distributed on all the seven cucumber chromosomes. Of these 18 loci, only six (dmG1.4, dmG4.1, dmG4.3, dmG5.2, dmG7.1, and dmG7.2) were detected in two experiments, and were considered as loci with a stable effect on DM resistance. Further, 16 out of the 18 loci colocalized with the QTLs that were reported in previous studies and two loci, dmG2.1 and dmG7.1, were novel ones identified only in this study. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlation analysis, several candidate genes were identified as potential causal genes underlying the stable and novel loci, including Csa1G575030 for dmG1.4, Csa2G060360 for dmG2.1, Csa4G064680 for dmG4.1, Csa5G606470 for dmG5.2, and Csa7G004020 for dmG7.1. This study shows that the CG germplasm is a very valuable resource carrying known and novel QTLs for DM resistance. The potential of using these CG lines for future allele-mining of candidate genes was discussed in the context of breeding cucumber with resistance to DM.
Responses to Salt Stress in Portulaca: Insight into Its Tolerance Mechanisms
Authors: Borsai Orsolya , Hassan Mohamad Al , Negrușier Cornel , Raigón M. Dolores , Boscaiu Monica , Sestraș Radu E. , Vicente Oscar
Published: November 2020

Climate change and its detrimental effects on agricultural production, freshwater availability and biodiversity accentuated the need for more stress-tolerant varieties of crops. This requires unraveling the underlying pathways that convey tolerance to abiotic stress in wild relatives of food crops, industrial crops and ornamentals, whose tolerance was not eroded by crop cycles. In this work we try to demonstrate the feasibility of such strategy applying and investigating the effects of saline stress in different species and cultivars of Portulaca. We attempted to unravel the main mechanisms of stress tolerance in this genus and to identify genotypes with higher tolerance, a procedure that could be used as an early detection method for other ornamental and minor crops. To investigate these mechanisms, six-week-old seedlings were subjected to saline stress for 5 weeks with increasing salt concentrations (up to 400 mM NaCl). Several growth parameters and biochemical stress markers were determined in treated and control plants, such as photosynthetic pigments, monovalent ions (Na+, K+ and Cl−), different osmolytes (proline and soluble sugars), oxidative stress markers (malondialdehyde—a by-product of membrane lipid peroxidation—MDA) and non-enzymatic antioxidants (total phenolic compounds and total flavonoids). The applied salt stress inhibited plant growth, degraded photosynthetic pigments, increased concentrations of specific osmolytes in both leaves and roots, but did not induce significant oxidative stress, as demonstrated by only small fluctuations in MDA levels. All Portulaca genotypes analyzed were found to be Na+ and Cl− includers, accumulating high amounts of these ions under saline stress conditions, but P. grandiflora proved to be more salt tolerant, showing only a small reduction under growth stress, an increased flower production and the lowest reduction in K+/Na+ rate in its leaves.
Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests
Authors: Poveda Jorge , Francisco Marta , Cartea M. Elena , Velasco Pablo
Published: November 2020

The Brassica genus includes one of the 10 most agronomically and economically important plant groups in the world. Within this group, we can find examples such as broccoli, cabbage, cauliflower, kale, Brussels sprouts, turnip or rapeseed. Their cultivation and postharvest are continually threatened by significant stresses of biotic origin, such as pathogens and pests. In recent years, numerous research groups around the world have developed transgenic lines within the Brassica genus that are capable of defending themselves effectively against these enemies. The present work compiles all the existing studies to date on this matter, focusing in a special way on those of greater relevance in recent years, the choice of the gene of interest and the mechanisms involved in improving plant defenses. Some of the main transgenic lines developed include coding genes for chitinases, glucanases or cry proteins, which show effective results against pathogens such as Alternaria brassicae, Leptosphaeria maculans or Sclerotinia sclerotiorum, or pests such as Lipaphis erysimi or Plutella xylostella.
Melt Memory Effects in Poly(butylene succinate) Studied by Differential Fast Scanning Calorimetry
Authors: Sangroniz Leire , Ocando Connie , Cavallo Dario , Müller Alejandro J.
Published: November 2020

It is widely accepted that melt memory effect on polymer crystallization depends on thermal history of the material, however a systematic study of the different parameters involved in the process has been neglected, so far. In this work, poly(butylene succinate) has been selected to analyze the effect of short times and high cooling/heating rates that are relevant from an industrial point of view by taking advantage of fast scanning calorimetry (FSC). The FSC experiments reveal that the width of melt memory temperature range is reduced with the time spent at the self-nucleation temperature (Ts), since annealing of crystals occurs at higher temperatures. The effectiveness of self-nuclei to crystallize the sample is addressed by increasing the cooling rate from Ts temperature. The effect of previous standard state on melt memory is analyzed by (a) changing the cooling/heating rate and (b) applying successive self-nucleation and annealing (SSA) technique, observing a strong correlation between melting enthalpy or crystallinity degree and the extent of melt memory. The acquired knowledge can be extended to other semicrystalline polymers to control accurately the melt memory effect and therefore, the time needed to process the material and its final performance.
Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells
Authors: Lungu Iulia Ioana , Nistorescu Simona , Badea Mădălina Andreea , Petre Andreea-Mihaela , Udrea Ana-Maria , Banici Ana-Maria , Fleacă Claudiu , Andronescu Ecaterina , Dinischiotu Anca , Dumitrache Florian , Staicu Angela , Balaș Mihaela
Published: November 2020

Even today, breast cancer remains a global public problem, with a high mortality rate among women. Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. By using iron oxide nanoparticles for cancer treatment, several advantages were highlighted: the ability to target specific locations derived from their magnetic properties and reduced side effects. The aim of this study was to examine on breast cancer cell line the anticancer potential of γ-Fe2O3 NPs loaded with doxorubicin (DOX) and stabilized with carboxymethylcellulose sodium (CMCNa). The γ-Fe2O3 NPs were synthesized by laser pyrolysis technique and their nanometric size and crystallinity were confirmed by X-ray diffraction and transmission electron microscopy. The loading efficiency was estimated by using absorption and fluorescence spectroscopy. The DOX conjugated//CMCNa coated γ-Fe2O3 NPs proved through the biological studies to have a good anticancer effect through the inhibition of tumoral cell proliferation, disruption of the cellular membrane, induction of cell death and reduced effects on normal breast cells. Our data showed that DOX cytotoxicity increases significantly when conjugated with ɣ-Fe2O3 and ɣ-Fe2O3_CMCNa, a 50% reduction of cancer cell viability was obtained with a concentration around 0.1 µg/mL.
Validation of a Simulation Methodology for Thermoplastic and Thermosetting Composite Materials Considering the Effect of Forming Process on the Structural Performance
Authors: Sisca Lorenzo , Locatelli Quacchia Patrizio Tiziano , Messana Alessandro , Airale Andrea Giancarlo , Ferraris Alessandro , Carello Massimiliana , Monti Marco , Palenzona Marta , Romeo Andrea , Liebold Christian , Scalera Salvatore , Festa Alberto , Codrino Paolo
Published: November 2020

This research work investigated the influence of the press molding manufacturing process on the mechanical properties, both for thermoplastic and thermosetting fiber reinforced composite materials. The particular geometry of the case study, called Double Dome, was considered in order to verify the behavior of the Thermoplastic and Thermosetting prepreg in terms of shell thickness variation and fibers shear angle evolution during the thermoforming process. The thermoforming simulation was performed using LS-DYNA® Finite Element Analysis (FEA) code, and the results were transferred by Envyo®, a dedicated mapping tool, into a LS-DYNA® virtual model for the structural simulation. A series of Double Dome specimens was produced with industrial equipment, and a bending experimental test was been carried on. Finally, a numerical-experimental correlation was performed, highlighting a significant forecast of the mechanical properties for the considered component.
Effect of Site-Specific Functionalization on the Shape of Nonspherical Block Copolymer Particles
Authors: Shin Jaeman J.
Published: November 2020

Shape-anisotropic polymeric colloids having chemically distinct compartments are promising materials, however, introducing site-specific surface functionality to block copolymer (BCP) particles has not yet been actively investigated. The current contribution demonstrates the selective surface functionalization of nanostructured, ellipsoidal polystyrene-b-polybutadiene (PS-b-PB) particle and investigate their effects on the particle shape. Photo-induced thiol-ene click reaction was used as a selective functionalization chemistry for modifying the PB block, which was achieved by controlling the feed ratio of functional thiols to the double bonds in PB. Importantly, the controlled particle elongation was observed as a function of the degree of PB functionalization. Such an increase in the aspect ratio is attributed to the (i) increased incompatibility of the PS and modified PB block and (ii) the reduced surface tension between the particles and surrounding aqueous medium, both of which contributes to the further elongation of ellipsoids. Further tunability of the elongation behavior of ellipsoids was further demonstrated by controlling the particle size and chemical structure of functional thiols, showing the versatility of this approach for controlling the particle shape. Finally, the utility of surface functionality was demonstrated by the facile complexation of fluorescent dye on the modified surface of the particle via favorable interaction, which showed stable fluorescence and colloidal dispersity.
FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
Authors: Melčová Veronika , Svoradová Kateřina , Menčík Přemysl , Kontárová Soňa , Rampichová Michala , Hedvičáková Věra , Sovková Věra , Přikryl Radek , Vojtová Lucy
Published: November 2020

Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition Modeling (FDM) based on plasticized poly(3-hydroxybutyrate)/poly(d,l-lactide) 70/30 blend modified with tricalcium phosphate bioceramics were prepared. Two different plasticizers, Citroflex (n-Butyryl tri-n-hexyl citrate) and Syncroflex (oligomeric adipate ester), both used in the amount of 12 wt%, were compared. The printing parameters for these materials were optimized and the printability was evaluated by recently published warping test. The samples were studied with respect to their thermal and mechanical properties, followed by biological in vitro tests including proliferation, viability, and osteogenic differentiation of human mesenchymal stem cells. According to the results from differential scanning calorimetry and tensile measurements, the Citroflex-based plasticizer showed very good softening effect at the expense of worse printability and unsatisfactory performance during biological testing. On the other hand, the samples with Syncroflex demonstrated lower warping tendency compared to commercial polylactide filament with the warping coefficient one third lower. Moreover, the Syncroflex-based samples exhibited the non-cytotoxicity and promising biocompatibility.
Progress of Interfacial Polymerization Techniques for Polyamide Thin Film (Nano)Composite Membrane Fabrication: A Comprehensive Review
Authors: Seah Mei Qun , Lau Woei Jye , Goh Pei Sean , Tseng Hui-Hsin , Wahab Roswanira Abdul , Ismail Ahmad Fauzi
Published: November 2020

In this paper, we review various novel/modified interfacial polymerization (IP) techniques for the fabrication of polyamide (PA) thin film composite (TFC)/thin film nanocomposite (TFN) membranes in both pressure-driven and osmotically driven separation processes. Although conventional IP technique is the dominant technology for the fabrication of commercial nanofiltration (NF) and reverse osmosis (RO) membranes, it is plagued with issues of low membrane permeability, relatively thick PA layer and susceptibility to fouling, which limit the performance. Over the past decade, we have seen a significant growth in scientific publications related to the novel/modified IP techniques used in fabricating advanced PA-TFC/TFN membranes for various water applications. Novel/modified IP lab-scale studies have consistently, so far, yielded promising results compared to membranes made by conventional IP technique, in terms of better filtration efficiency (increased permeability without compensating solute rejection), improved chemical properties (crosslinking degree), reduced surface roughness and the perfect embedment of nanomaterials within selective layers. Furthermore, several new IP techniques can precisely control the thickness of the PA layer at sub-10 nm and significantly reduce the usage of chemicals. Despite the substantial improvements, these novel IP approaches have downsides that hinder their extensive implementation both at the lab-scale and in manufacturing environments. Herein, this review offers valuable insights into the development of effective IP techniques in the fabrication of TFC/TFN membrane for enhanced water separation.
Impact of Effluent from the Leachate Treatment Plant of Taman Beringin Solid Waste Transfer Station on the Quality of Jinjang River
Authors: Chin Pui Mun , Naim Aine Nazira , Suja Fatihah , Ahmad Usul Muhammad Fadly
Published: November 2020

Rapid population growth has contributed to increased solid waste generated in Malaysia. Most landfills that have reached the design capacity are now facing closure. Taman Beringin Landfill was officially closed, so the Taman Beringin Solid Waste Transfer Station was built to manage the relocation, consolidation, and transportation of solid waste to Bukit Tagar Sanitary Landfill. Leachates are generated as a consequence of rainwater percolation through waste and biochemical processes in waste cells. Leachate treatment is needed, as leachates cause environmental pollution and harm human health. This study investigates the impact of treated leachate discharge from a Leachate Treatment Plant (LTP) on the Jinjang River water quality. The performance of the LTP in Taman Beringin Solid Waste Transfer Station was also assessed. Leachate samples were taken at the LTP’s anoxic tank, aeration tank, secondary clarifier tank, and final discharge point, whereas river water samples were taken upstream and downstream of Jinjang River. The untreated leachate returned the following readings: biochemical oxygen demand (BOD) (697.50 ± 127.94 mg/L), chemical oxygen demand (COD) (2419.75 ± 1155.22 mg/L), total suspended solid (TSS) (2710.00 ± 334.79 mg/L), and ammonia (317.08 ± 35.45 mg/L). The LTP’s overall performance was satisfactory, as the final treated leachates were able to meet the standard requirements of the Environmental Quality (Control of Pollution from Solid Waste Transfer Station and Landfill) Regulation 2009. However, the LTP’s activated sludge system performance was not satisfactory, and the parameters did not meet the standard limits. The result shows a low functioning biological treatment method that could not efficiently treat the leachate. However, a subsequent step of combining the biological and chemical process (coagulation, flocculation, activated sludge system, and activated carbon adsorption) helped the treated leachate to meet the standard B requirement stipulated by the Department of Environment (DOE), i.e., to flow safely into the river. This study categorized Jinjang River as polluted, with the discharge of the LTP’s treated leachates, possibly contributing to the river pollution. However, other factors, such as the upstream sewage treatment plant and the ex-landfill downstream, may have also affected the river water quality. The LTP’s activated sludge system performance at the transfer station still requires improvement to reduce the cost of the chemical treatment.
Predicting By-Product Gradients of Baker’s Yeast Production at Industrial Scale: A Practical Simulation Approach
Authors: Sarkizi Shams Hajian Christopher , Haringa Cees , Noorman Henk , Takors Ralf
Published: November 2020

Scaling up bioprocesses is one of the most crucial steps in the commercialization of bioproducts. While it is known that concentration and shear rate gradients occur at larger scales, it is often too risky, if feasible at all, to conduct validation experiments at such scales. Using computational fluid dynamics equipped with mechanistic biochemical engineering knowledge of the process, it is possible to simulate such gradients. In this work, concentration profiles for the by-products of baker’s yeast production are investigated. By applying a mechanistic black-box model, concentration heterogeneities for oxygen, glucose, ethanol, and carbon dioxide are evaluated. The results suggest that, although at low concentrations, ethanol is consumed in more than 90% of the tank volume, which prevents cell starvation, even when glucose is virtually depleted. Moreover, long exposure to high dissolved carbon dioxide levels is predicted. Two biomass concentrations, i.e., 10 and 25 g/L, are considered where, in the former, ethanol production is solely because of overflow metabolism while, in the latter, 10% of the ethanol formation is due to dissolved oxygen limitation. This method facilitates the prediction of the living conditions of the microorganism and its utilization to address the limitations via change of strain or bioreactor design or operation conditions. The outcome can also be of value to design a representative scale-down reactor to facilitate strain studies.
InSAR- and PIM-Based Inclined Goaf Determination for Illegal Mining Detection
Authors: Xia Yuanping , Wang Yunjia
Published: November 2020

The determination of the depth and boundary of the goaf is of great significance for the detection of illegal mining. However, determining the current location of unknown goafs mainly relies on low-efficiency, time-consuming, and labor-intensive physical detection methods such as geomagnetic field changes. Due to their large coverage and high degree of automation, research on remote sensing methods has been conducted to locate mining activities by monitoring surface deformation. This paper proposes a method that relies on the principle of the probability integration method (PIM) and on synthetic aperture radar interferometry (InSAR) to retrieve the location of an underground goaf. First, the relationship between ground subsidence and the location of the mined-out area was established according to PIM; then, the location of the mined-out area was obtained by the surface deformation acquired by InSAR. The proposed method does not rely on complex nonlinear models and has complete parameters; therefore, it has higher engineering application value. A test site in the Fengfeng mining area and 11 Radarsat-2 images were used to verify the proposed method. The experimental results showed that the average relative error of the proposed method is 6.35%, which is 27.56% higher than that of similar algorithms based on complex nonlinear models. Compared to algorithms that ignore the coal seam dip, the accuracy is improved to 98.27%.