bluuebunny/crossref_metadata_2025 huggingface dataset:
Vagus nerve stimulation: mechanisms and factors involved in memory enhancement
Authors: Olsen Laura K. , Solis Ernesto , McIntire Lindsey K. , Hatcher-Solis Candice N.
Published: June 2023

Vagus nerve stimulation (VNS) has been recognized as a useful neuromodulation tool to target the central nervous system by electrical stimulation of peripheral nerves. Activation of the nucleus of the solitary tract (NTS) in the brainstem by vagal afferent nerve fibers allows for modulation of various higher order brain regions, including limbic and cerebral cortex structures. Along with neurological and psychiatric indications, clinical and preclinical studies suggest that VNS can improve memory. While the underlying mechanisms to improve memory with VNS involve brain areas, such as the prefrontal cortex and processes including alertness and arousal, here we focus on VNS-induced memory improvements related to the hippocampus, the main area implicated in memory acquisition. In addition, we detail research demonstrating that a targeted approach to VNS can modify memory outcomes and delve into the molecular mechanisms associated with these changes. These findings indicate that a greater understanding of VNS mechanisms while also considering stimulation parameters, administration site, timing in relation to training, and sex-specific factors, may allow for optimal VNS application to enhance memory.
Visuo-spatial imagery in dreams of congenitally and early blind: a systematic review
Authors: Ilic Katarina , Bertani Rita , Lapteva Neda , Drakatos Panagis , Delogu Alessio , Raheel Kausar , Soteriou Matthew , Mutti Carlotta , Steier Joerg , Carmichael David W. , Goadsby Peter J. , Ockelford Adam , Rosenzweig Ivana
Published: June 2023

Background

The presence of visual imagery in dreams of congenitally blind people has long been a matter of substantial controversy. We set to systematically review body of published work on the presence and nature of oneiric visuo-spatial impressions in congenitally and early blind subjects across different areas of research, from experimental psychology, functional neuroimaging, sensory substitution, and sleep research.

Methods

Relevant studies were identified using the following databases: EMBASE, MEDLINE and PsychINFO.

Results

Studies using diverse imaging techniques and sensory substitution devices broadly suggest that the “blind” occipital cortex may be able to integrate non-visual sensory inputs, and thus possibly also generate visuo-spatial impressions. Visual impressions have also been reported by blind subjects who had near-death or out-of-body experiences.

Conclusion

Deciphering the mechanistic nature of these visual impression could open new possibility in utilization of neuroplasticity and its potential role for treatment of neurodisability.
Case Report: SMARCA4 (BRG1)-deficient undifferentiated carcinoma of gallbladder with genetic analysis
Authors: Meng Xiangpeng , Ma Jia , Meng Nan , Yun Tianyu , Niu Beifang
Published: June 2023

SMARCA4 (BRG1)-deficient undifferentiated carcinoma is a rare and highly aggressive malignancy. It has been reported to occur in a multiple range of organs. However, to the best of our knowledge, SMARCA4 (BRG1)-deficient undifferentiated carcinoma of gallbladder has not yet been reported. Here, we describe a case of SMARCA4 (BRG1)-deficient undifferentiated carcinoma of gallbladder. Through comprehensive genetic analysis, we hypothesized that in addition to SMARCA4 (BRG1) deficiency, other genetic changes might also be involved in the tumorigenesis of undifferentiated gallbladder cancer in this patient, particularly somatic mutations in the CTNNB1, KRAS, PIK3CA, TP53, CREBBP, and FANCI genes. To the best of our knowledge, this is the first report of SMARCA4 (BRG1)-deficient undifferentiated carcinoma of gallbladder with genetic analysis.
Paper-based biosensors as point-of-care diagnostic devices for the detection of cancers: a review of innovative techniques and clinical applications
Authors: Mahmoodpour Mehrdad , Kiasari Bahman Abedi , Karimi Merat , Abroshan Arezou , Shamshirian Danial , Hosseinalizadeh Hamed , Delavari Alireza , Mirzei Hamed
Published: June 2023

The development and rapid progression of cancer are major social problems. Medical diagnostic techniques and smooth clinical care of cancer are new necessities that must be supported by innovative diagnostic methods and technologies. Current molecular diagnostic tools based on the detection of blood protein markers are the most common tools for cancer diagnosis. Biosensors have already proven to be a cost-effective and accessible diagnostic tool that can be used where conventional laboratory methods are not readily available. Paper-based biosensors offer a new look at the world of analytical techniques by overcoming limitations through the creation of a simple device with significant advantages such as adaptability, biocompatibility, biodegradability, ease of use, large surface-to-volume ratio, and cost-effectiveness. In this review, we covered the characteristics of exosomes and their role in tumor growth and clinical diagnosis, followed by a discussion of various paper-based biosensors for exosome detection, such as dipsticks, lateral flow assays (LFA), and microfluidic paper-based devices (µPADs). We also discussed the various clinical studies on paper-based biosensors for exosome detection.
An online tool for survival prediction of extrapulmonary small cell carcinoma with random forest
Authors: Zhang Xin
Published: June 2023

Purpose

Extrapulmonary small cell carcinoma (EPSCC) is rare, and its knowledge is mainly extrapolated from small cell lung carcinoma. Reliable survival prediction tools are lacking.

Methods

A total of 3,921 cases of EPSCC were collected from the Surveillance Epidemiology and End Results (SEER) database, which form the training and internal validation cohorts of the survival prediction model. The endpoint was an overall survival of 0.5–5 years. Internal validation performances of machine learning algorithms were compared, and the best model was selected. External validation (n = 68) was performed to evaluate the generalization ability of the selected model.

Results

Among machine learning algorithms, the random forest model performs best on internal validation, whose area under the curve (AUC) is 0.736–0.800. The net benefit is higher than the TNM classification in decision curve analysis. The AUC of this model on the external validation cohort is 0.739–0.811. This model was then deployed online as a free, publicly available prediction tool of EPSCC (http://42.192.80.13:4399/).

Conclusion

This study provides an excellent online survival prediction tool for EPSCC with machine learning and large-scale data. Age, TNM stages, and surgery (including potential performance status information) are the most critical factors for the prediction model.
Re-irradiation for head and neck cancer: outcome and toxicity analysis using a prospective single institution database
Authors: Scolari Chiara , Buchali André , Franzen Achim , Förster Robert , Windisch Paul , Bodis Stephan , Zwahlen Daniel R. , Schröder Christina
Published: June 2023

Purpose

Re-irradiation (re-RT) in head and neck cancer is challenging. This study prospectively explored the feasibility of re-RT in patients with loco-regionally recurrent or second primary head and neck cancer (LRR/SP HNC).

Methods

From 2004 to 2021, 61 LRR/SP HNC patients were treated with re-RT, defined as having a second course of RT with curative intent resulting in a cumulative dose of ≥100 Gy in an overlapping volume. Postoperative or definitive dynamic intensity-modulated and/or volumetric modulated re-RT was administered using twice daily hyperfractionation to 60 Gy combined with cisplatin or carboplatin/5-fluorouracil. Overall survival (OS), progression-free survival (PFS), locoregional control (LRC) and distant metastasis control (DMC) were analyzed and prognostic factors evaluated. Toxicity was prospectively recorded and graded.

Results

The median follow-up was 9.8 months. In 41 patients (67.1%), complete administration of the intended treatment was not feasible. In 9 patients (15%) re-RT was interrupted prematurely and in other 9, the complete re-RT dose was lower than 60 Gy, and 37 patients (61%) could not receive or complete chemotherapy. Two-year OS, PFS and LRC rates were 19%, 18% and 30%, respectively. 20 patients (33%) received the complete intended treatment, and 1- and 2-year OS rates were 70% and 47%, respectively. Charlson comorbidity index was an important predictor for treatment completion. Multivariate analysis revealed recurrent N stage 0–1, age, chemotherapy administration and re-RT dose of 60 Gy as prognostic factors for clinical outcomes. No grade 5 re-RT-related toxicity was observed. The most common new grade ≥3 acute toxicities were dysphagia (52%) and mucositis (46%). Late toxicity included grade ≥3 dysphagia in 5% and osteoradionecrosis in 10% of evaluable patients, respectively. 6 patients (10%) were alive after 9 years without progression and no late toxicity grade ≥3, except for 2 patients presenting with osteoradionecrosis.

Conclusion

Hyperfractionated re-RT with 60 Gy combined with platinum-based chemotherapy was a curative treatment option with acceptable toxicity in LRR/SP patients. Patients with higher comorbidity had a higher probability of failing to receive and complete the intended therapy. Consequently, they derived unsatisfactory benefits from re-RT, highlighting the importance of patient selection.
Pretreatment lymphocyte-to-monocyte ratio as a prognostic factor and influence on dose-effect in fractionated stereotactic radiotherapy for oligometastatic brain metastases in non-small cell lung cancer patients
Authors: Chen Tian , Tang Mengqiu , Zhou Yang , Wang Zhepei , Li Shiwei , Wang Hongcai , Lu Yangfang , Wang Jinguo , Shen Weiyu
Published: June 2023

Background

Studies on the prognostic factors for patients with brain oligo-metastasis treated with fractionated stereotactic radiotherapy (FSRT) usually focus on the size of metastatic tumor and radiation dose. Some inflammatory indicators have predictive value in non-small cell lung cancer (NSCLC) with brain metastasis receiving stereotactic radiotherapy. However, the prognostic value of inflammatory indicators in NSCLC patients with brain oligo-metastasis treated with FSRT, and their effect on radiotherapy dose is unknown.

Methods

A total of 95 advanced NSCLC patients with brain oligo-metastasis who had undergone FSRT treatment at Ningbo Medical Center Lihuili Hospital between January 2015 and April 2022 were enrolled into the study. Neutrophil to lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), lymphocyte to monocyte ratio (LMR), tumor diameter and biologically effective dose (BED10) were analyzed using Chi-square test. Univariate and multivariate Cox regressions were used to identify predictors of survival.

Results

Tumor diameter (< 2 cm), BED10 (≥ 48Gy) and LMR (≥ 4) were found to be independently associated with good intracranial local control survival (i-LCS) through multivariate analysis. The median i-LCS was longer in patients with 2 independent risk factors (tumor diameter ≥ 2 and LMR < 4) administered with BED10 > 53.6Gy compared with patients administered with BED10 ≤ 53.6Gy (20.7 months vs 12.0 months, P = 0.042). LMR ≥ 4 (P = 0.019) and positivity for driver gene mutations (P = 0.011) were independently associated with better overall survival (OS).

Conclusions

LMR is an independent prognostic factor of i-LCS and OS in NSCLC patients with brain oligo-metastasis treated with FSRT. Patients with tumor diameter ≥ 2 and LMR < 4 should be treated with BED10 greater than 53.6Gy.
Role of interleukin 1β and interleukin 10 variants on ocular toxoplasmosis in Brazilian individuals
Authors: Araujo Warlen Miiller Rocha , Ayo Christiane Maria , Previato Mariana , de Faria Geraldo Magela , Frederico Fábio Batista , Siqueira Rubens Camargo , de Almeida Gildásio Castello , Pereira-Chioccola Vera Lúcia , de Mattos Luiz Carlos , Brandão Cinara Cássia
Published: June 2023

Introduction

Ocular toxoplasmosis (OT) is an intraocular inflammation caused by Toxoplasma gondii infection that affects the retina and choroid, giving rise to posterior uveitis. Genetic polymorphisms in cytokine genes may exert influence in the expression of these molecules and play a significant role in inflammatory responses and susceptibility to OT. The aim of this study was to evaluate the role of polymorphisms rs16944 (–511 C > T) of the interleukin (IL) 1β gene and rs1800896 (–1082 G > A) of the IL10 gene on OT in Brazilian individuals with a serologic diagnosis of T. gondii and after conducting fundoscopic exams.

Methods

Participants with a positive serology were classified into two distinct groups according to the presence (G1; n = 110) or absence (G2; n = 104) of OT. The control group (G3) consisted of individuals without the infection (n = 108).

Results

It was observed that the C/C genotype of the IL1β gene polymorphism was a protective factor for OT (p = 0.02, OR = 0.28, 95% CI 0.08–0.78 for G1 vs. G2; p = 0.03; OR = 0.29, 95% CI 0.09–0.82 for G1 vs. G3), according to the recessive inheritance model.

Conclusions

The -511C.T polymorphisms of the IL1β gene seems to play an important role in the pathogenesis of OT in Brazilian individuals.
Evaluation of safety and effectiveness of remdesivir in treating COVID-19 patients after emergency use authorization study
Authors: Burhan Erlina , Syahruddin Elisna , Isbaniah Fathiyah , Desianti Ginanjar Arum , Fachrucha Fanny , Sari Cut Yulia Indah , Ismail Efriadi , Astuti Puji , Maruli Muhammad Farhan , Mubarak Farhan , Rengganis Anggit Tresna , Bilqis Hazia Hanifa , Taslim Imammurahman , Sastria Evan , Wiyarta Elvan
Published: June 2023

Background: This study aimed to determine the real-world safety and effectiveness of remdesivir in hospitalized adult COVID-19 patients with moderate-to-critical disease in Indonesia.

Methods: A multicenter, retrospective cohort study was conducted at four COVID-19 referral hospitals in Jakarta. A total of 587 patients were included, of whom 243 received remdesivir within 72 h of admission. The safety endpoints were the proportions of patients with any adverse event (AE), any grade 3 AE, and AE of each system organ class. The effectiveness endpoints were ICU admission >24 h from baseline, live discharge and mortality at day 14, live discharge and mortality at day 28, and virologic conversion. Patients who received remdesivir within 72 h of admission were considered the treatment group, and those who did not were the control group. Multivariate adjustments were performed using a modified Poisson regression.

Results: The study found no significant differences in safety endpoints between the two groups. However, the effectiveness endpoints showed that remdesivir was associated with a decreased risk of ICU admission >24 h from baseline (RR 0.71, 95% CI 0.52–0.96), an increased probability of live discharge at day 14 (RR 1.37, 95% CI 1.08–1.74), and an increased probability of live discharge at day 28 (RR 1.28, 95% CI 1.05–1.57). The rate of virologic conversion was not significantly different between the two groups.

Conclusion: The study concludes that remdesivir is safe and effective in the treatment of moderate-to-critical COVID-19 in a real-world setting in Indonesia.
Voltage-gated sodium channels: from roles and mechanisms in the metastatic cell behavior to clinical potential as therapeutic targets
Authors: Sanchez-Sandoval Ana Laura , Hernández-Plata Everardo , Gomora Juan Carlos
Published: June 2023

During the second half of the last century, the prevalent knowledge recognized the voltage-gated sodium channels (VGSCs) as the proteins responsible for the generation and propagation of action potentials in excitable cells. However, over the last 25 years, new non-canonical roles of VGSCs in cancer hallmarks have been uncovered. Their dysregulated expression and activity have been associated with aggressive features and cancer progression towards metastatic stages, suggesting the potential use of VGSCs as cancer markers and prognostic factors. Recent work has elicited essential information about the signalling pathways modulated by these channels: coupling membrane activity to transcriptional regulation pathways, intracellular and extracellular pH regulation, invadopodia maturation, and proteolytic activity. In a promising scenario, the inhibition of VGSCs with FDA-approved drugs as well as with new synthetic compounds, reduces cancer cell invasion in vitro and cancer progression in vivo. The purpose of this review is to present an update regarding recent advances and ongoing efforts to have a better understanding of molecular and cellular mechanisms on the involvement of both pore-forming α and auxiliary β subunits of VGSCs in the metastatic processes, with the aim at proposing VGSCs as new oncological markers and targets for anticancer treatments.
Development and economic assessment of machine learning models to predict glycosylated hemoglobin in type 2 diabetes
Authors: Tong Yi-Tong , Gao Guang-Jie , Chang Huan , Wu Xing-Wei , Li Meng-Ting
Published: June 2023

Background: Glycosylated hemoglobin (HbA1c) is recommended for diagnosing and monitoring type 2 diabetes. However, the monitoring frequency in real-world applications has not yet reached the recommended frequency in the guidelines. Developing machine learning models to screen patients with poor glycemic control in patients with T2D could optimize management and decrease medical service costs.

Methods: This study was carried out on patients with T2D who were examined for HbA1c at the Sichuan Provincial People’s Hospital from April 2018 to December 2019. Characteristics were extracted from interviews and electronic medical records. The data (excluded FBG or included FBG) were randomly divided into a training dataset and a test dataset with a radio of 8:2 after data pre-processing. Four imputing methods, four screening methods, and six machine learning algorithms were used to optimize data and develop models. Models were compared on the basis of predictive performance metrics, especially on the model benefit (MB, a confusion matrix combined with economic burden associated with therapeutic inertia). The contributions of features were interpreted using SHapley Additive exPlanation (SHAP). Finally, we validated the sample size on the best model.

Results: The study included 980 patients with T2D, of whom 513 (52.3%) were defined as positive (need to perform the HbA1c test). The results indicated that the model trained in the data (included FBG) presented better forecast performance than the models that excluded the FBG value. The best model used modified random forest as the imputation method, ElasticNet as the feature screening method, and the LightGBM algorithms and had the best performance. The MB, AUC, and AUPRC of the best model, among a total of 192 trained models, were 43475.750 (¥), 0.972, 0.944, and 0.974, respectively. The FBG values, previous HbA1c values, having a rational and reasonable diet, health status scores, type of manufacturers of metformin, interval of measurement, EQ-5D scores, occupational status, and age were the most significant contributors to the prediction model.

Conclusion: We found that MB could be an indicator to evaluate the model prediction performance. The proposed model performed well in identifying patients with T2D who need to undergo the HbA1c test and could help improve individualized T2D management.
Adaptive plasmonic metasurfaces for radiative cooling and passive thermoregulation
Authors: Didari-Bader Azadeh , Estakhri Nooshin M. , Mohammadi Estakhri Nasim
Published: June 2023

In this work, we investigate a class of planar photonic structures operating as passive thermoregulators. The radiative cooling process is adjusted through the incorporation of a phase change material (Vanadium Dioxide, VO2) in conjunction with a layer of transparent conductive oxide (Aluminum-doped Zinc Oxide, AZO). VO2 is known to undergo a phase transition from the “dielectric” phase to the “plasmonic” or “metallic” phase at a critical temperature close to 68°C. In addition, AZO shows plasmonic properties at the long-wave infrared spectrum, which, combined with VO2, provides a rich platform to achieve low reflections across the atmospheric transparency window, as demanded in radiative cooling applications, while also maintaining a compact size. Using numerical analysis, we study two classes of patterned and non-patterned compact multilayer metal-dielectric-metal metasurfaces, aiming to maximize the overall absorption in the first atmospheric transparency window (8–13 µm) while maintaining a high reflection across the solar spectrum (0.3–2.5 µm). Surfaces are initially designed based on a round of coarse optimization and further improved through analyzing the impact of geometric parameters such as size and periodicity of the metasurface elements. Our findings are relevant to applications in thermal regulation systems and passive radiative cooling of high-temperature devices, such as electronic elements.
Training drills in high performance badminton—effects of interval duration on internal and external loads
Authors: Edel Antonia , Weis Jan-Luka , Ferrauti Alexander , Wiewelhove Thimo
Published: June 2023

Purpose: The aim of the present study was to analyze the impact of interval duration on training loads and technical skill performance in high performance badminton drills.

Methods: On three experimental days, 19 internationally ranked players (13 male: 22.7 ± 3.8 years, 180 ± 6 cm, 71.5 ± 6.1 kg; 6 females: 20.4 ± 2.5 years, 168 ± 4 cm, 59.8 ± 6.0 kg) completed one of three protocols (T10, T30, and T50) of a typical badminton specific drill, the so-called “Multifeeding” (the coach feeds shuttlecock without break in a random order) in a counterbalanced order. The protocols varied in interval duration (10, 30, and 50 s) but were matched for the rally-to-rest-ratio (1:1) and active playing time (600 s). Cardiorespiratory responses (portable spirometry, chest belt), energy metabolism (levels of blood lactate, La), rate of perceived exertion (RPE), player’s kinematics (Local Positioning System), and technical skill performance (video analysis) were measured.

Results: Average oxygen consumption (T10 45 ± 6; T30 46 ± 7; T50 44 ± 6 mL min−1·kg−1), Energy expenditure (886 ± 209; 919 ± 176; 870 ± 206 kcal h−1), heart rate (164 ± 13; 165 ± 11; 165 ± 10 bpm) and RPE (16 ± 2; 17 ± 2; 17 ± 2) did not differ between the protocols. Respiratory exchange ratio (RER) and La significantly increased depending on interval duration (RER: 0.90 ± 0.05; 0.93 ± 0.03; 0.96 ± 0.04 and La: 3.6 ± 2.0; 5.6 ± 3.0; 7.3 ± 2.3 mmol l−1). Stroke frequency (SF; 0.58 ± 0.05; 0.57 ± 0.05; 0.55 ± 0.06 strokes·s−1) was similar while distance covered, and average running velocity were significantly lower for T50 compared to T10 (76 ± 17; 70 ± 13; 65 ± 11 m min−1). Moreover, jump frequency in T30 was higher than in T10 (6.7 ± 3.1; 8.8 ± 3.8; 8.5 ± 4.2 jumps·min−1), whereas differences in internal and external loads were not associated with changes in stroke precision (errors: 16 ± 6; 19 ± 4; 18 ± 4%; accuracy: 22 ± 6; 24 ± 8; 23 ± 8%).

Conclusion: Anaerobic metabolic stimulus increases while running distance and velocity decrease, in case of longer interval durations. Even though there was no impact on stroke precision, extending the intervals beyond 30 s might impair external training load and skill performance. Consequently, interval duration should be defined carefully depending on the training goals.
“Single-pole dual-control” competing mode in plants
Authors: Yu Tian-Ying , Gao Tian-Ying , Li Wen-Jia , Cui Dan-Lu
Published: June 2023

Plant development and pattern formation depend on diffusible signals and location cues. These developmental signals and cues activate intracellular downstream components through cell surface receptors that direct cells to adopt specific fates for optimal function and establish biological fitness. There may be a single-pole dual-control competing mode in controlling plant development and microbial infection. In plant development, paracrine signaling molecules compete with autocrine signaling molecules to bind receptors or receptor complexes, turn on antagonistic molecular mechanisms, and precisely regulate developmental processes. In the process of microbial infection, two different signaling molecules, competing receptors or receptor complexes, form their respective signaling complexes, trigger opposite signaling pathways, establish symbiosis or immunity, and achieve biological adaptation. We reviewed several “single-pole dual-control” competing modes, focusing on analyzing the competitive commonality and characterization of “single-pole dual-control” molecular mechanisms. We suggest it might be an economical protective mechanism for plants’ sequentially and iteratively programmed developmental events. This mechanism may also be a paradigm for reducing internal friction in the struggle and coexistence with microbes. It provides extraordinary insights into molecular recognition, cell-to-cell communication, and protein–protein interactions. A detailed understanding of the “single-pole dual-control” competing mode will contribute to the discovery of more receptors or antagonistic peptides, and lay the foundation for food, biofuel production, and crop improvement.
Elevated fruit nitrogen impairs oil biosynthesis in olive (Olea europaea L.)
Authors: Erel Ran , Yermiyahu Uri , Yasuor Hagai , Ben-Gal Alon , Zipori Isaac , Dag Arnon
Published: June 2023

Oil in fruits and seeds is an important source of calories and essential fatty acids for humans. This specifically holds true for olive oil, which is appreciated for its superior nutritional value. Most olive orchards are cultivated to produce oil, which are the outcome of fruit yield and oil content. Little information is available on the effect of nitrogen (N) on olive fruit oil content. The response of olive trees to different rates of N was therefore studied in soilless culture (3 years) and commercial field (6 years) experiments. In both experiments, fruit N level and oil biosynthesis were negatively associated. Fruit N increased in response to N fertilization level and was inversely related to fruit load. The negative correlation between fruit N and oil content was more pronounced under high fruit load, indicating sink limitation for carbon. These results agree with those reported for oilseed crops for which a trade-off between oil and protein was proposed as the governing mechanism for the negative response to elevated N levels. Our results suggest that the protein/oil trade-off paradigm cannot explain the noticeable decrease in oil biosynthesis in olives, indicating that additional mechanisms are involved in N-induced inhibition of oil production. This inhibition was not related to the soluble carbohydrate levels in the fruit, which were comparable regardless of N level. These results emphasize the importance of balanced N nutrition in oil-olive cultivation to optimize production with oil content.
Effect of strong wind on laminas and petioles of Farfugium japonicum (L.) Kitam. var. japonicum (Asteraceae)
Authors: Shiba Masayuki , Mizuno Tsukumo , Fukuda Tatsuya
Published: June 2023

Farfugium japonicum (L.) Kitam. var. japonicum grows mainly in the coastal areas of Japan. Meteorological recording data from natural habitats were used to investigate the factors associated with the laminas and petioles of radical leaves of F. japonicum var. japonicum to avoid or resist higher wind stress. Our morphological and mechanical results indicated that petiole length and petiole cross-sectional area had a weak correlation with wind speed and breaking strength, and the petiole second area moment of inertia did not differ significantly among populations. However, both lamina area and petiole length per petiole cross-sectional area decreased with increasing wind speed, indicating that F. japonicum var. japonicum resisted or avoided an increase in wind speed outdoors by reducing the lamina area and petiole length per petiole cross-sectional area without qualitative changes in their petioles. The results of this study indicated that densely distributed recording stations of the Automated Meteorological Data Acquisition System (AMeDAS) by the Japan Meteorological Agency can be used for environmental adaptation studies of plants in the field using nearby plant populations.
CsMYB15 positively regulates Cs4CL2-mediated lignin biosynthesis during juice sac granulation in navel orange
Authors: Song Fang , Li Zixuan , Wang Ce , Jiang Yingchun , Wang Zhijing , He Ligang , Ma Xiaofang , Zhang Yu , Song Xin , Liu Jihong , Wu Liming
Published: June 2023

‘Lane Late’, a late-maturing navel orange cultivar, is mainly distributed in the Three Gorges Reservoir area, which matures in the late March of the next year and needs overwintering cultivation. Citrus fruit granulation is a physiological disorder, which is characterized by lignification and dehydration of juice sac cells, seriously affecting the commercial value of citrus fruits. The pre-harvest granulation of late-maturing navel orange is main caused by low temperature in the winter, but its mechanism and regulation pattern remain unclear. In this study, a SG2-type R2R3-MYB transcription factor, CsMYB15, was identified from Citrus sinensis, which was significantly induced by both juice sac granulation and low temperature treatment. Subcellular localization analysis and transcriptional activation assay revealed that CsMYB15 protein was localized to the nucleus, and it exhibited transcriptional activation activity in yeast. Over-expression of CsMYB15 by stable transformation in navel orange calli and transient transformation in kumquat fruits and navel orange juice sacs significantly increased lignin content in the transgenic lines. Further, Yeast one hybrid, EMSA, and LUC assays demonstrated that CsMYB15 directly bound to the Cs4CL2 promoter and activated its expression, thereby causing a high accumulation of lignin in citrus. Taken together, these results elucidated the biological function of CsMYB15 in regulating Cs4CL2-mediated lignin biosynthesis, and provided novel insight into the transcriptional regulation mechanism underlying the juice sac granulation of late-maturing navel orange.
Deliberation and polarization: a multi-disciplinary review
Authors: Caluwaerts Didier , Bernaerts Kamil , Kesberg Rebekka , Smets Lien , Spruyt Bram
Published: June 2023

In recent years, deliberative democracy has drawn attention as a potential way of fighting polarization. Allowing citizens to exchange arguments and viewpoints on political issues in group, can have strong conflict-mitigating effects: it can foster opinion changes (thereby overcoming idea-based polarization), and improve relations between diametrically opposed groups (thereby tackling affective forms of polarization, such as affective polarization). However, these results conflict with social psychological and communication studies which find that communicative encounters between groups can lead to further polarization and even group think. The question therefore arises under which conditions deliberative interactions between citizens can decrease polarization. Based on a multidisciplinary systematic review of the literature, which includes a wide diversity of communicative encounters ranging from short classroom discussions to multi-weekend citizen assemblies, this paper reports several findings. First, we argue that the effects of communicative encounters on polarization are conditional on how those types of communication were conceptualized across disciplines. More precisely, we find depolarizing effects when group discussions adhere to a deliberative democracy framework, and polarizing effects when they do not. Second we find that the depolarizing effects depend on several design factors that are often implemented in deliberative democracy studies. Finally, our analysis shows that that much more work needs to be done to unravel and test the exact causal mechanism(s) underlying the polarization-reducing effects of deliberation. Many potential causal mechanisms were identified, but few studies were able to adjudicate how deliberation affects polarization.
What makes a theme park experience less enjoyable? Evidence from online customer reviews of Disneyland China
Authors: Bai Shizhen , He Hao , Han Chunjia , Yang Mu , Bi Xinrui , Fan Weijia
Published: June 2023

The aim of this study was to explore the causes of tourists’ lesser enjoyment of theme parks through an unsupervised machine learning approach—structural topic modelling. Specifically, the research extracted a comprehensive list of discussion topics about the travel experience of tourists through the analysis of 112,000 customer reviews released by visitors to the Shanghai Disney Resort from 16 June 2016 to 4 March 2022. Then, we used sentiment analysis to distinguish positive and negative topics and to explore the relationship between tourists who buy different ticket types and sentiments in negative topics. The results show that problems such as “parking,” “service attitude,” “recommendation feeling,” “experience comparison,” and “entrance” may be the main reasons for an unpleasant experience. In addition, we also found that when tourists travel in groups (e.g., via family tickets), customers feel unhappy because of parking and fast track problems. Moreover, when tourists travel alone or with small groups, staff service attitudes, experience comparisons, and entrance processes are the sources of greater concern. Our findings can help theme park managers to better understand the expectations of tourists and take effective measures to tackle issues causing customer dissatisfaction, and they can also contribute to theme park studies in tourism management.
Person-centered care: preferences and predictors in speech-language pathology and audiology practitioners
Authors: Mahomed-Asmail Faheema , Hlayisi Vera-Genevey , Joubert Karin , Metcalfe Louise Anne , Graham Marien Alet , Swanepoel De Wet
Published: June 2023

Introduction

Increasingly person-centered care (PCC) is being recognized as an important aspect of speech-language pathology and audiology (SLP/A) service delivery. This study aimed to (i) identify preferences toward PCC; (ii) determine predictors of these preferences; and (iii) describe the understanding and views of PCC among SLP/A in South Africa.

Methods

A mixed-method design was followed utilizing an online survey and four focus group discussions. The survey included demographic questions, the modified Patient-Practitioner Orientation Scale (mPPOS), the Ten-Item-Personality-Inventory (TIPI) and an open-ended question. The focus group discussions included prompting questions which facilitated an open-ended discussion.

Results

A total of 91 practitioners (39.6% speech-language pathologists) completed the online survey, with nine (44.4% audiologists) participating in the focus group discussions. A high preference toward PCC was noted, with a total mean mPPOS score of 4.6 (0.6 SD). Quantile regression analysis revealed four predictors (age, home language, sector, and personality trait openness) associated with PCC preferences. Three main categories emerged from the open-ended question and focus group discussions: (i) Positive experiences with PCC; (ii) restrictions toward PCC, and (iii) PCC exposure.

Discussion

Positive (age and personality trait openness) and negative (home language and sector of employment) predictors toward PCC exist among speech-language pathologists and audiologists, with an overall general preference toward PCC. Practitioners experience facilitators and barriers toward implementing PCC including the extent of personal experiences, available resources and tools as well as workplace culture. These aspects require further investigation.
Effects of an outdoor horticultural activities program on cognitive and behavioral functioning, mood, and perceived quality of life in people with dementia: a pilot study
Authors: Borella Erika , Melendugno Andrea , Meneghetti Chiara , Murroni Veronica , Carbone Elena , Goldin Giulia , Cavalli Raffaele , Basso Andrea , Pazzaglia Francesca
Published: June 2023

Introduction

One of various non-pharmacological treatments for people with dementia (PwD) is horticultural therapy. The aim of this double-blind, pre- and post-test, pilot study was to examine the effects of horticultural activities (HA) for PwD at a residential and daytime care facility. Whether combining HA with elements drawn from other psychosocial interventions (cognitive stimulation) would maximize any benefits was also newly examined.

Materials and methods

Twenty-four PwD were involved either in HA, alone (TG1, N = 7) or combined with some cognitive stimulation (TG2, N = 8), or in indoor treatment-as-usual activities (CG, N = 9). Benefits were assessed in terms of general cognitive functioning (for participants with mild-to-moderate dementia), mood, behavioral and psychological symptoms, and quality of life.

Results

No differences emerged between TG1 and TG2 in any outcome measure, so the two groups were combined (N = 15). Compared with the CG, the TG involved in HA exhibited less frequent and severe behavioral and psychological symptoms and an improved mood after the intervention. Caregivers also reported less distress in the TG after the intervention than in the CG. Considering only PwD with mild-to-moderate dementia, the TG also showed benefits in a measure of general cognitive functioning, and self-reported quality of life, compared with the CG.

Discussion

Our results further confirm that engaging PwD in participatory HA in contact with natural elements can decrease their dementia symptoms and their caregivers’ distress, but also increase PwD’s quality of life. Our findings also suggest the need to consider dementia severity when assessing the benefits of horticultural therapy.
Stress overload, influencing factors, and psychological experiences of nurse managers during early stages of the COVID-19 pandemic: a sequential explanatory mixed method study
Authors: Jin Yundan , Cui Feifei , Wang Rongting , Chen Shuainan , Hu Lina , Yao Meiqi , Wu Haiying
Published: June 2023

Background

Healthcare systems had an exceptionally difficult time during the early COVID-19 pandemic. Nurse managers in particular made enormous contributions to ensuring the safety of patients and front-line nurses while being under excessive psychological stress. However, little is known about their experiences during this time.

Objective

The aim of this study was thus to assess the level of stress overload and psychological feelings of nurse managers during the early COVID-19 pandemic.

Methods

A mixed methods sequential explanatory design study with non-random convenience sampling was performed, following the STROBE and COREQ checklists. The study was conducted at the Affiliated Dongyang Hospital, Wenzhou Medical University, with data collected from six provinces in southern China (Zhejiang, Hubei, Shanghai, Jiangsu, Hunan and Jiangxi) during March 2020 and June 2020. A total of 966 nurse managers completed the Stress Overload Scale and Work-Family Support Scale. In addition, a nested sample of nurse managers participated in semi-structured face-to-face interviews. The data were then analyzed using qualitative content analysis, Pearson correlation, and multiple linear regression.

Results

The quantitative results showed that nurse managers experienced a moderate level of stress load. There was a significant negative correlation between work-family support and stress load (r = −0.551, p < 0.01). Concerns about protecting front-line nurses and work-family support were the main factors affecting the stress load, which accounted for 34.0% of the total variation. Qualitative analysis identified four main thematic analyses that explained stress load: (1) great responsibility and great stress, (2) unprecedented stress-induced stress response, (3) invisible stress: the unknown was even more frightening, and (4) stress relief from love and support. Taken together these findings indicate that concern about protecting front-line nurses and negative work-family support of nurse managers were the main factors causing stress overload.

Conclusion

Implementing measures focused on individual psychological adjustment combined with community and family support and belongingness is one potential strategy to reduce psychological stress among nurse managers.
The development of the revised COPE 68 inventory with English and Slovak versions
Authors: Halamová Júlia , Kanovský Martin , Strnádelová Bronislava , Baránková Martina , Greškovičová Katarína
Published: June 2023

Introduction

Although there have been several attempts at improving the COPE Inventory, the factor structure of the instrument is still in dispute. In addition, studies have shown low reliability coefficients for some of the first-order factors, with Mental Disengagement having the lowest factor loadings. In a recent study on the external validation of the instrument, two additional first-order factors were identified in the qualitative analysis, namely Self-care and Care for Others.

Methods

Based on these arguments we created the Revised COPE 68 Inventory, changing some of the problematic items in the first order factor Mental Disengagement and adding items for the two new factors (Self-care and Care for Others). We then tested its reliability and performed factor analyses on the first and second-order factorial structure. The data were collected through social media in two languages, English and Slovak, using convenience and snowball sampling techniques. The English sample contained 834 participants with mean age 25.27  years (SD = 8.467) and the Slovak sample comprised 1,425 participants with mean age 33 years (SD = 14.59). For the statistical analyses we used Exploratory Structural Equation Model (ESEM) analyses with target rotation and WLSMV, Exploratory and second-order confirmatory factor analysis with the scores of the COPE Inventory and EFA.

Results

The Revised COPE 68 inventory had a good fit for all 17 first-order factors in both languages, including for the new factors Self-care and Care for Others. It appears that the first-order factors form a three-factor solution in both samples, consisting of active coping, social–emotional coping and avoidant coping. The revised Mental Disengagement has better psychometric properties as well.

Discussion

The Revised COPE 68 inventory was found to be a reliable multidimensional instrument for measuring various coping strategies in both the English and Slovak language versions.
The role of emotion processing in art therapy (REPAT) intervention protocol
Authors: Czamanski-Cohen Johanna , Weihs Karen L.
Published: June 2023

Psychological and physical health are known to improve with emotion processing, which is becoming aware of bodily sensations, accepting them as information that can be translated into emotion concepts and expressing them symbolically and linguistically as emotions. Art therapy utilizes the visual arts for processing emotions to facilitate self-expression and communication with the goal of improving psychological wellbeing. The mental health of individuals coping with and recovering from cancer is known to benefit from art therapy. The purpose of this paper is to describe the development of the role of emotion processing in art therapy (REPAT) intervention, which is an 8 week, one and a half hour art therapy intervention created to target emotion processing as a primary mechanism of change, through which art therapy has the potential to reduce symptoms (i.e., depression, pain and fatigue) of women coping with breast cancer. To obtain this goal we used template for intervention description and replication (TIDieR) and GUIDance for the rEporting of intervention Development (GUIDED) guidelines for intervention development description, with the goal of ensuring successful implementation for clinical and research use.
Emotional and behavioral alterations and their relationship with executive functioning in the pediatric population with autism spectrum disorder and epilepsy: a comparative study
Authors: Cano-Villagrasa Alejandro , Moya-Faz Francisco J. , Cortés-Ramos Antonio , López-Zamora Miguel
Published: June 2023

Introduction

The pediatric population with Autism Spectrum Disorder (ASD) and epilepsy presents behavioral and emotional alterations that hinder their correct developmental maturation as well as their integration in different contexts such as school, family or social. This population shows atypical behavioral and emotional patterns, with difficulties in emotional regulation, understanding of emotions, aggressiveness, or low frustration tolerance. They also present alterations in executive functions, which significantly influence the emotional and behavioral characteristics of this population. Research suggests that epilepsy worsens the emotional, behavioral, and executive functioning status.

Objective

To explore differences in behavioral, emotional, and executive functioning profile in individuals with a diagnosis of ASD, epilepsy, and ASD with epilepsy.

Method

In this quasi-experimental and cross-sectional study, a total of 170 participants were selected and distributed into three groups: a group of participants with ASD, a group with epilepsy, and a group of participants with ASD and epilepsy. The SENA, BASC-3, and ENFEN tests were administered to verify the behavioral, emotional, and executive functioning profile in the three groups.

Results

The results indicate that individuals diagnosed with ASD and epilepsy present greater emotional, behavioral, and executive functioning alterations compared to those who only present ASD or epilepsy.

Conclusion

Individuals with ASD and epilepsy present significant alterations in emotional, behavioral, and executive functioning processes, which hinder their adaptation to different contexts, as well as decreasing their quality of life and that of their family.
Association between depression and macrovascular disease: a mini review
Authors: Zhao Shuwu , Zhu Liping , Yang Jinfeng
Published: June 2023

Depression and macrovascular diseases are globally recognized as significant disorders that pose a substantial socioeconomic burden because of their associated disability and mortality. In addition, comorbidities between depression and macrovascular diseases have been widely reported in clinical settings. Patients afflicted with coronary artery disease, cerebrovascular disease or peripheral artery disease exhibit an elevated propensity for depressive symptoms. These symptoms, in turn, augment the risk of macrovascular diseases, thereby reflecting a bidirectional relationship. This review examines the physiological and pathological mechanisms behind comorbidity while also examining the intricate connection between depression and macrovascular diseases. The present mechanisms are significantly impacted by atypical activity in the hypothalamic–pituitary–adrenal axis. Elevated levels of cortisol and other hormones may disrupt normal endothelial cell function, resulting in vascular narrowing. At the same time, proinflammatory cytokines like interleukin-1 and C-reactive protein have been shown to disrupt the normal function of neurons and microglia by affecting blood–brain barrier permeability in the brain, exacerbating depressive symptoms. In addition, platelet hyperactivation or aggregation, endothelial dysfunction, and autonomic nervous system dysfunction are important comorbidity mechanisms. Collectively, these mechanisms provide a plausible physiological basis for the interplay between these two diseases. Interdisciplinary collaboration is crucial for future research aiming to reveal the pathogenesis of comorbidity and develop customised prevention and treatment strategies.