bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Frequency of Fresh Pasture Allocation on Pasture Utilisation and the Performance of High Yielding Dairy Cows
Authors: Pollock Jessica G. , Gordon Alan W. , Huson Kathryn M. , McConnell Deborah A.
Published: November 2020

Pasture allocation frequency (PAF) can influence pasture availability and grazing behaviour, which subsequently may impact on animal performance. Limited research to-date has investigated grazing management methods to improve the performance of high production dairy cows whilst also achieving high grass utilisation rates. This study evaluated the effect of three different PAF’s (12, 24 and 36 h) on pasture utilisation, the performance of high yielding dairy cows and the interaction with parity. The experiment included two 60-day periods, 90 spring calving dairy cows (27 primiparous animals) in period one and 87 (24 primiparous animals) in period two. The average pre-grazing sward height (11.4 cm) was similar for all treatments in both periods. In period one, pasture utilisation rate was significantly higher (8%) in the 36 h compared to the 12 h treatment. In period two, milk energy output was significantly greater for primiparous animals in the 36 h treatment relative to the other treatments.
Effects of Roughage Quality and Particle Size on Rumen Parameters and Fatty Acid Profiles of Longissimus Dorsi Fat of Lambs Fed Complete Feed
Authors: Matar Abdulkareem M. , Abdelrahman Mutassim M. , Alhidary Ibrahim A. , Ayadi Moez A. , Alobre Mohsen M. , Aljumaah Riyadh S.
Published: November 2020

The fatty acid composition for the longissimus dorsi (LD) fat of carcass sheep is a crucial factor impacting meat quality. We performed a 90-day feeding trial of 25 Naemi lambs to investigate the effects of roughage sources (alfalfa or wheat straw) of two sizes (regular and 1 cm chopped) when fed with pelleted total mixed ration (TMR) on the growth performance, fermentation patterns, and fatty acid (FA) composition of longissimus dorsi (LD) fat. Lambs were randomly assigned to individual pens with five treatment diets, as follows: C, control group with TMR; T1, TMR and regular alfalfa hay; T2, TMR and alfalfa hay chopped to 1 cm; T3, TMR and regular wheat straw; and T4, TMR and wheat straw chopped to 1 cm. Four lambs were randomly selected from each treatment (20 total) and sacrificed. LD fat of the carcass was extracted and analyzed for FA using a gas chromatography-mass spectrometry. Significantly increased feed intake was found in T1 and T2. The FA composition of LD fat in T2 had higher unsaturated fatty acid (UFA), omega-6 (n6), and omega-3 (n3) FA content. Conjugated linoleic acid (CLA) and α-linoleic acid were highest in lambs fed T1 and T2. Feeding different types of roughage, especially alfalfa hay, either regular or chopped, with total pelleted mixed ration is crucial to improving feed intake and body weight gain, as it positively enhances the rumen microbial fermentation process by controlling rumen pH. The FA profiles of meat from lambs fed TMR with regular or 1 cm particle size alfalfa hay (T1 and T2) are recommended for human consumption as a source of healthy FAs.
Alleviation of the Adverse Effect of Dietary Carbohydrate by Supplementation of Myo-Inositol to the Diet of Nile Tilapia (Oreochromis niloticus)
Authors: Zhu Jiahua , Pan Jingyu , Wang Xiaodan , Huang Yuxing , Qin Chuanjie , Qiao Fang , Qin Jianguang , Chen Liqiao
Published: November 2020

This study investigated the effect of dietary myo-inositol (MI) on alleviating the adverse effect of the high carbohydrate diet in Nile tilapia (Oreochromis niloticus). Six diets contained either low carbohydrate (LC 30%) or high carbohydrate (HC 45%) with three levels of MI supplementation (0, 400 and 1200 mg/kg diet) to each level of the carbohydrate diet. After an 8-week trial, the fish fed 400 mg/kg MI under HC levels had the highest weight gain and fatness, but the fish fed 1200 mg/kg MI had the lowest hepatosomatic index, visceral index and crude lipid in the HC group. The diet of 1200 mg/kg MI significantly decreased triglyceride content in the serum and liver compared with those fed the MI supplemented diets regardless of carbohydrate levels. Dietary MI decreased triglyceride accumulation in the liver irrespective of carbohydrate levels. The content of malondialdehyde decreased with increasing dietary MI at both carbohydrate levels. Fish fed 1200 mg/kg MI had the highest glutathione peroxidase, superoxide dismutase, aspartate aminotransferase and glutamic-pyruvic transaminase activities. The HC diet increased the mRNA expression of key genes involved in lipid synthesis (DGAT, SREBP, FAS) in the fish fed the diet without MI supplementation. Dietary MI significantly under expressed fatty acid synthetase in fish fed the HC diets. Moreover, the mRNA expression of genes related to lipid catabolism (CPT, ATGL, PPAR-α) was significantly up-regulated with the increase of dietary MI levels despite dietary carbohydrate levels. The gene expressions of gluconeogenesis, glycolysis and MI biosynthesis were significantly down-regulated, while the expression of the pentose phosphate pathway was up-regulated with the increase of MI levels. This study indicates that HC diets can interrupt normal lipid metabolism and tend to form a fatty liver in fish. Dietary MI supplement can alleviate lipid accumulation in the liver by diverging some glucose metabolism into the pentose phosphate pathway and enhance the antioxidant capacity in O. niloticus.
A Descriptive Study of Keel Bone Fractures in Hens and Roosters from Four Non-Commercial Laying Breeds Housed in Furnished Cages
Authors: Kittelsen Käthe Elise , Moe Randi Oppermann , Hansen Tone Beate , Toftaker Ingrid , Christensen Jens Peter , Vasdal Guro
Published: November 2020

The presence of keel bone fractures (KBF) in laying hens has been documented and discussed by several authors, nevertheless the causative factors behind KBF remain uncertain. High prevalence of KBF have been reported in all commercial egg production systems, in different genetic lines and at different ages. Several of the proposed causal mechanisms behind KBF are linked to selection for efficient production. It is, therefore, of interest to explore whether less selected breeds have a lower occurrence of keel bone fractures compared to reports from highly selected, modern laying hen breeds. Thus, the aim of the current study was to investigate keel bones of hens from four non-commercial layer breeds. Birds were housed in furnished cages and keel bones examined at 30 and 63 weeks of age, using a portable X-ray equipment. The results from this descriptive study indicate a low prevalence of KBF at both ages in all four breeds, with only five KBF detected in 213 X-ray pictures taken from 126 birds. Of these, four of the KBF were observed in the most genetically selected breed, with an early onset of lay. None of the roosters examined exhibited KBF. The overall low numbers of KBF found indicate that genetic factors may be involved in KBF and, thus that selective breeding may help to reduce the susceptibility to KBF. Finally, this study highlights the importance of poultry conservation to secure genetic diversity, which may be an important resource in future selection schemes.
Effect of Phlorotannins from Brown Seaweeds on the In Vitro Digestibility of Pig Feed
Authors: Ford Lauren , Curry Chloe , Campbell Mairead , Theodoridou Katerina , Sheldrake Gary , Dick Jaimie , Stella Lorenzo , Walsh Pamela J.
Published: November 2020

Phlorotannins have been reported to have positive effects on pig health, including improved gut health and digestibility. In this study, we investigate the effect of phenolics found in two brown seaweeds, Ascophyllum nodosum and Fucus serratus, on in vitro dry matter digestibility of seaweeds and commercial pig feed. Phlorotannin extracts and whole seaweeds were supplemented into pig feed to test their effect on digestibility. Solid-phase extraction was used to purify the phenolics to phlorotannins. The results showed a slight decrease in the digestibility of pig feed that was found to be significant when phlorotannin extracts were added from either seaweed. However, when whole A. nodosum was added to the pig feed, the effect on digestibility was less pronounced. Specifically, no significant difference in digestibility was observed at inclusion rates up to 5%, and thereafter results varied. A difference in digestibility was also observed in the same species at the same inclusion rate, collected from different seasons. This suggests that other compounds, e.g., polysaccharides, are having an effect on digestibility when whole seaweeds are supplemented to animal feed. This research has also highlighted the need to base supplementation on phenolic concentration as opposed to a standardised percentage inclusion of seaweeds to ensure that digestibility is not adversely affected.
Sequential Hypertonic-Hypotonic Treatment Enhances Efficacy of Antibiotic against Acinetobacter baumannii Biofilm Communities
Authors: Falghoush Azeza , Beyenal Haluk , Call Douglas R.
Published: November 2020

Infections with bacterial biofilm communities are highly tolerant of antibiotics. This protection is attributed, in part, to a hydrated extracellular polymeric substance (EPS) that surrounds the bacterial community and that limits antibiotic diffusion. In this study, we evaluated whether it is possible to dehydrate and then re-hydrate a biofilm as a means to increase antibiotic penetration and efficacy. Acinetobacter baumannii biofilms (24 h) were exposed to hypertonic concentrations of maltodextrin, sucrose or polyethylene glycol (PEG) as the dehydration step. These biofilms were then washed with deionized water containing 10 times the concentration of antibiotics needed to kill these bacteria in broth culture (50 µg/mL tobramycin, 300 µg/mL chloramphenicol, 20 µg/mL ciprofloxacin or 100 µg/mL erythromycin) as the rehydration step. Biofilms were then harvested, and the number of viable cells was determined. Sequential treatment with PEG and tobramycin reduced cell counts 4 to 7 log (p < 0.05) relative to combining PEG and tobramycin in a single treatment, and 3 to 7 log relative to tobramycin treatment alone (p < 0.05). Results were variable for other osmotic compounds and antibiotics depending on the concentrations used, likely related to mass and hydrophobicity. Our findings support future clinical evaluation of sequential regimens of hypertonic and hypotonic solutions to enhance antibiotic efficacy against chronic biofilm infections.
Antimicrobial Resistance Patterns in Organic and Conventional Dairy Herds in Sweden
Authors: Sjöström Karin , Hickman Rachel A. , Tepper Viktoria , Olmos Antillón Gabriela , Järhult Josef D. , Emanuelson Ulf , Fall Nils , Sternberg Lewerin Susanna
Published: November 2020

Monitoring antimicrobial resistance (AMR) and use (AMU) is important for control. We used Escherichia coli from healthy young calves as an indicator to evaluate whether AMR patterns differ between Swedish organic and conventional dairy herds and whether the patterns could be related to AMU data. Samples were taken twice, in 30 organic and 30 conventional dairy herds. Selective culturing for Escherichia coli, without antibiotics and with nalidixic acid or tetracycline, was used to estimate the proportions of resistant isolates. Microdilution was used to determine the minimum inhibitory concentrations (MICs) for thirteen antimicrobial substances. AMU data were based on collection of empty drug packages. Less than 8% of the bacterial growth on non-selective plates was also found on selective plates with tetracycline, and 1% on plates with nalidixic acid. Despite some MIC variations, resistance patterns were largely similar in both periods, and between organic and conventional herds. For most substances, only a few isolates were classified as resistant. The most common resistances were against ampicillin, streptomycin, sulfamethoxazole, and tetracycline. No clear association with AMU could be found. The lack of difference between organic and conventional herds is likely due to a generally good animal health status and consequent low AMU in both categories.
Dopamine Therapy and the Regulation of Oxidative Stress and Mitochondrial DNA Copy Number in Patients with Parkinson’s Disease
Authors: Chen Shih-Hsuan , Kuo Chung-Wen , Lin Tsu-Kung , Tsai Meng-Han , Liou Chia-Wei
Published: November 2020

Few studies have reported on changes to oxidative stress and mitochondrial DNA copy numbers in patients with Parkinson’s disease (PD), particularly those undergoing long-term dopamine therapy. This study measured mitochondrial copy numbers, thiobarbituric acid reactive substances (TBARS), and thiols in 725 PD patients and 744 controls. The total prescribed dopamine dose was calculated for each PD patient. A decreased mitochondrial copy number and antioxidant thiols level, but an elevated oxidative TBARS level presented in PD patients. Stratification into age subgroups revealed a consistently lower mitochondrial copy number and thiols in all PD subgroups, but increased TBARS levels compared with those of the controls. Further study found an association between lower serum TBARS and dopamine administration. There appears to be an indirect relationship with the mitochondrial copy number, where a decrease in TBARS was found to diminish the effect of pathogenetic and age-related decrease in mitochondrial copy number in PD patients. Follow-up evaluations noted more significant decreases of mitochondrial copy numbers in PD patients over time; meanwhile, dopamine administration was associated with an initial decrease of the TBARS level which attenuated with high-dose and long-term therapy. Our study provides evidence that moderate dopamine dose therapy benefits PD patients through attenuation of oxidative stress and manipulation of the mitochondrial copy number.
Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice
Authors: Méndez-Lara Karen , Letelier Nicole , Farré Núria , Diarte-Añazco Elena , Nieto-Nicolau Núria , Rodríguez-Millán Elisabeth , Santos David , Pallarès Victor , Escolà-Gil Joan , Vázquez del Olmo Tania , Lerma Enrique , Camacho Mercedes , Casaroli-Marano Ricardo , Valledor Annabel , Blanco-Vaca Francisco , Julve Josep
Published: November 2020

The potential of nicotinamide (NAM) to prevent atherosclerosis has not yet been examined. This study investigated the effect of NAM supplementation on the development of atherosclerosis in a mouse model of the disease. The development of aortic atherosclerosis was significantly reduced (NAM low dose: 45%; NAM high dose: 55%) in NAM-treated, apolipoprotein (Apo)E-deficient mice challenged with a Western diet for 4 weeks. NAM administration significantly increased (1.8-fold) the plasma concentration of proatherogenic ApoB-containing lipoproteins in NAM high-dose (HD)-treated mice compared with untreated mice. However, isolated ApoB-containing lipoproteins from NAM HD mice were less prone to oxidation than those of untreated mice. This result was consistent with the decreased (1.5-fold) concentration of oxidized low-density lipoproteins in this group. Immunohistochemical staining of aortas from NAM-treated mice showed significantly increased levels of IL-10 (NAM low-dose (LD): 1.3-fold; NAM HD: 1.2-fold), concomitant with a significant decrease in the relative expression of TNFα (NAM LD: −44%; NAM HD: −57%). An improved anti-inflammatory pattern was reproduced in macrophages cultured in the presence of NAM. Thus, dietary NAM supplementation in ApoE-deficient mice prevented the development of atherosclerosis and improved protection against ApoB-containing lipoprotein oxidation and aortic inflammation.
Sinapic Acid Inhibits Cardiac Hypertrophy via Activation of Mitochondrial Sirt3/SOD2 Signaling in Neonatal Rat Cardiomyocytes
Authors: Yun Ui Jeong , Yang Dong Kwon
Published: November 2020

Sinapic acid (SA) is a naturally occurring phenolic compound with antioxidant properties. It also has a wide range of pharmacological properties, such as anti-inflammatory, anticancer, and hepatoprotective properties. The present study aimed to evaluate the potential pharmacological effects of SA against hypertrophic responses in neonatal rat cardiomyocytes. In order to evaluate the preventive effect of SA on cardiac hypertrophy, phenylephrine (PE)-induced hypertrophic cardiomyocytes were treated with subcytotoxic concentrations of SA. SA effectively suppressed hypertrophic responses, such as cell size enlargement, sarcomeric rearrangement, and fetal gene re-expression. In addition, SA significantly inhibited the expression of mitogen-activated protein kinase (MAPK) proteins as pro-hypertrophic factors and protected the mitochondrial functions from hypertrophic stimuli. Notably, SA activated Sirt3, a mitochondrial deacetylase, and SOD2, a mitochondrial antioxidant, in hypertrophic cardiomyocytes. SA also inhibited oxidative stress in hypertrophic cardiomyocytes. However, the protective effect of SA was significantly reduced in Sirt3-silenced hypertrophic cardiomyocytes, indicating that SA exerts its beneficial effect through Sirt3/SOD signaling. In summary, this is the first study to reveal the potential pharmacological action and inhibitory mechanism of SA as an antioxidant against cardiac hypertrophy, suggesting that SA could be utilized for the treatment of cardiac hypertrophy.
Polyphenolic and Methylxanthine Bioaccessibility of Cocoa Bean Shell Functional Biscuits: Metabolomics Approach and Intestinal Permeability through Caco-2 Cell Models
Authors: Rojo-Poveda Olga , Barbosa-Pereira Letricia , El Khattabi Charaf , Youl Estelle N.H. , Bertolino Marta , Delporte Cédric , Pochet Stéphanie , Stévigny Caroline
Published: November 2020

Cocoa bean shell (CBS), a by-product with considerable concentrations of bioactive compounds and proven biofunctional potential, has been demonstrated to be a suitable ingredient for high-fiber functional biscuits adapted to diabetic consumers. In this work, the in vitro bioaccessibility and intestinal absorption of polyphenols and methylxanthines contained in these biscuits were evaluated, and the effect of the food matrix was studied. Biscuits containing CBS and the CBS alone underwent in vitro digestion followed by an intestinal permeability study. The results confirmed that compounds were less bioavailable in the presence of a food matrix, although the digestion contributed to their release from this matrix, increasing the concentrations available at the intestinal level and making them capable of promoting antioxidant and antidiabetic activities. After digestion, CBS biscuits were shown to possess α-glucosidase inhibition capacity comparable to that of acarbose. Moreover, the presence of the food matrix improved the stability of polyphenols throughout the digestion process. Intestinal absorption of flavan-3-ols seemed to be limited to a maximum threshold and was therefore independent of the sample, while procyanidin was not absorbed. Methylxanthine absorption was high and was boosted by the presence of the food matrix. The results confirmed the biofunctional potential of CBS-based biscuits.
The Interplay between Oxidative Stress, Exercise, and Pain in Health and Disease: Potential Role of Autonomic Regulation and Epigenetic Mechanisms
Authors: Hendrix Jolien , Nijs Jo , Ickmans Kelly , Godderis Lode , Ghosh Manosij , Polli Andrea
Published: November 2020

Oxidative stress can be induced by various stimuli and altered in certain conditions, including exercise and pain. Although many studies have investigated oxidative stress in relation to either exercise or pain, the literature presents conflicting results. Therefore, this review critically discusses existing literature about this topic, aiming to provide a clear overview of known interactions between oxidative stress, exercise, and pain in healthy people as well as in people with chronic pain, and to highlight possible confounding factors to keep in mind when reflecting on these interactions. In addition, autonomic regulation and epigenetic mechanisms are proposed as potential mechanisms of action underlying the interplay between oxidative stress, exercise, and pain. This review highlights that the relation between oxidative stress, exercise, and pain is poorly understood and not straightforward, as it is dependent on the characteristics of exercise, but also on which population is investigated. To be able to compare studies on this topic, strict guidelines should be developed to limit the effect of several confounding factors. This way, the true interplay between oxidative stress, exercise, and pain, and the underlying mechanisms of action can be revealed and validated via independent studies.
Energy Recovery via Thermal Gasification from Waste Insulation Electrical Cables (WIEC)
Authors: Mota-Panizio Roberta , Hermoso-Orzáez Manuel Jesús , Carmo-Calado Luís , Campos Victor Arruda Ferraz de , Silveira José Luz , Gonçalves Maria Margarida , Brito Paulo
Published: November 2020

The recovery of noble metals from electrical wires and cables results in waste materials such as polyvinyl chloride (PVC) and polyethylene (PE), that is, waste insulation electrical cables (WIEC), which have been processed by gasification for energy recovery. This study focused on the effect of blending the ratio of WIEC on the gasification feedstock composition and the lower heating value (LHV) of produced syngas, through controlled tests and tests under different loads on the generator. The controlled gasification experiments were carried out at blending ratios between pine biomass and WIEC of 90:10, 80:20, and 70:30 and with pine biomass only (100%). For the loads gasification, the experiments were carried out at a blending ratio of 80:20. The controlled experimental results presented that the highest hydrogen content, approximated 17.7 vol.%, was observed at a blending ratio of 70:30 between pine biomass and WIEC and the highest LHV of syngas was observed at a blending ratio of 90:10, with 5.7 MJ/Nm3. For the load gasification experiments, the results showed that the highest hydrogen content was obtained with a load of 15 kW in the generator, approximately 18.48 vol.% of hydrogen content, and the highest LHV of synthesis gas was observed during the 5 kW test, with 5.22 MJ/Nm3. Overall, the new processing of waste insulation electrical cables using a downdraft gasification reactor demonstrates great promise for high quality syngas production.
Design and Modelling of a Novel Integrated Photonic Device for Nano-Scale Magnetic Memory Reading
Authors: Demirer Figen Ece , van den Bomen Chris , Lavrijsen Reinoud , van der Tol Jos J. G. M. , Koopmans Bert
Published: November 2020

Design and simulations of an integrated photonic device that can optically detect the magnetization direction of its ultra-thin (∼12 nm) metal cladding, thus ‘reading’ the stored magnetic memory, are presented. The device is an unbalanced Mach Zehnder Interferometer (MZI) based on InP Membrane on Silicon (IMOS) platform. The MZI consists of a ferromagnetic thin-film cladding and a delay line in one branch, and a polarization converter in the other. It quantitatively measures the non-reciprocal phase shift caused by the Magneto-Optic Kerr Effect in the guided mode which depends on the memory bit’s magnetization direction. The current design is an analytical tool for research exploration of all-optical magnetic memory reading. It has been shown that the device is able to read a nanoscale memory bit (400 × 50 × 12 nm) by using a Kerr rotation as small as 0.2∘, in the presence of a noise ∼10 dB in terms of signal-to-noise ratio. The device is shown to tolerate performance reductions that can arise during the fabrication.
The Power System and Microgrid Protection—A Review
Authors: Sahebkar Farkhani Jalal , Zareein Mohammad , Najafi Arsalan , Melicio Rui , Rodrigues Eduardo M. G.
Published: November 2020

In recent years, power grid infrastructures have been changing from a centralized power generation model to a paradigm where the generation capability is spread over an increasing number of small power stations relying on renewable energy sources. A microgrid is a local network including renewable and non-renewable energy sources as well as distributed loads. Microgrids can be operated in both grid-connected and islanded modes to fill the gap between the significant increase in demand and storage of electricity and transmission issues. Power electronics play an important role in microgrids due to the penetration of renewable energy sources. While microgrids have many benefits for power systems, they cause many challenges, especially in protection systems. This paper presents a comprehensive review of protection systems with the penetration of microgrids in the distribution network. The expansion of a microgrid affects the coordination and protection by a change in the current direction in the distribution network. Various solutions have been suggested in the literature to resolve the microgrid protection issues. The conventional coordination of the protection system is based on the time delays between relays as the primary and backup protection. The system protection scheme has to be changed in the presence of a microgrid, so several protection schemes have been proposed to improve the protection system. Microgrids are classified into different types based on the DC/AC system, communication infrastructure, rotating synchronous machine or inverter-based distributed generation (DG), etc. Finally, we discuss the trend of future protection schemes and compare the conventional power systems.
Learning-Free Text Line Segmentation for Historical Handwritten Documents
Authors: Kurar Barakat Berat , Cohen Rafi , Droby Ahmad , Rabaev Irina , El-Sana Jihad
Published: November 2020

We present a learning-free method for text line segmentation of historical handwritten document images. This method relies on automatic scale selection together with second derivative of anisotropic Gaussian filters to detect the blob lines that strike through the text lines. Detected blob lines guide an energy minimization procedure to extract the text lines. Historical handwritten documents contain noise, heterogeneous text line heights, skews and touching characters among text lines. Automatic scale selection allows for automatic adaption to the heterogeneous nature of handwritten text lines in case the character height range is correctly estimated. In the extraction phase, the method can accurately split the touching characters among the text lines. We provide results investigating various settings and compare the model with recent learning-free and learning-based methods on the cBAD competition dataset.
Ozone Treatment as a Process of Quality Improvement Method of Rhubarb (Rheum rhaponticum L.) Petioles during Storage
Authors: Zardzewiały Miłosz , Matlok Natalia , Piechowiak Tomasz , Gorzelany Józef , Balawejder Maciej
Published: November 2020

The aim of the study was to identify the effects of ozone treatment reflected by the microbial, mechanical properties and selected chemical parameters during the storage of rhubarb petioles. For this purpose, after they were harvested, rhubarb petioles were treated with gaseous ozone at concentrations of 10 and 100 ppm, for the duration of 5, 15 and 30 min. Subsequently, the plant material was stored at room temperature for 14 days. After this time, the raw material was subjected to a number of chemical and mechanical tests. It was shown that the rhubarb petioles treated with ozone at a rate of 100 ppm for 30 min were characterized by the lowest loss of water content. It was also found that, compared to the control, most samples subjected to ozone treatment presented better mechanical properties, as well as higher oxidative potential and contents of polyphenols and vitamin C. Based on these findings, it was determined that ozone treatment largely increases storage stability of rhubarb.
Anaerobic Digestion of Steam-Exploded Wheat Straw and Co-Digestion Strategies for Enhanced Biogas Production
Authors: Kaldis Fokion , Cysneiros Denise , Day James , G. Karatzas Kimon-Andreas , Chatzifragkou Afroditi
Published: November 2020

Wheat straw (WS) is considered a favourable substrate for biogas production. However, due to its rigid structure and high carbon to nitrogen (C/N ratio), its biodegradability during anaerobic digestion (AD) is usually low. In the present study, the effect of steam explosion pre-treatment on WS, combined with C/N adjustment with inorganic nitrogen, on biogas production was evaluated. Additionally, co-digestion of WS with protein-rich agri-industrial by-products (dried distillers’ grains with solubles (DDGS) and rapeseed meal (RM)) was assessed. Steam explosion enhanced biogas production from WS, whereas the addition of NH4Cl was beneficial (p < 0.05) for the digestion of steam-exploded wheat straw (SE). Furthermore, mono-digestion of the four different substrates seemed to be efficient in both inoculum to substrate ratios (I/S) tested (3.5 and 1.75 (w/w)). Finally, during co-digestion of WS and SE with DDGS and RM, an increase in the cumulative methane production was noted when higher amounts of DDGS and RM were co-digested. This study demonstrated that DDGS and RM can be used as an AD supplement to stimulate gas production and improve wheat straw biodegradability, while their addition at 10% on an AD system operating with WS can enhance gas yields at levels similar to those achieved by steam-exploded straw.
High-Yielding Lovastatin Producer Aspergillus terreus Shows Increased Resistance to Inhibitors of Polyamine Biosynthesis
Authors: Zhgun Alexander A. , Nuraeva Gulgina K. , Volkov Ivan A.
Published: November 2020

The biosynthesis of pharmaceutically significant secondary metabolites in filamentous fungi is a multistep process that depends on a wide range of various factors, one of which is the intracellular content of polyamines. We have previously shown that in Aspergillus terreus lovastatin high-yielding strain (HY) exogenous introduction of polyamines during fermentation can lead to an increase in the production of lovastatin by 20–45%. However, the molecular mechanisms of this phenomenon have not been elucidated. In this regard, we carried out an inhibitory analysis at the key stage of polyamine biosynthesis, the conversion of L-ornithine to putrescine by the enzyme ornithine decarboxylase (ODC). A. terreus HY strain showed upregulation of genes for biosynthesis of polyamines, 3–10-fold, and increased resistance compared to the original wild-type strain upon inhibition of ODC on synthetic medium with 5 mM α-difluoromethylornithine (DFMO), by 20–25%, and 5 mM 1-aminooxy-3-aminopropane (APA), by 40–45%. The data obtained indicate changes in the metabolism of polyamines in A. terreus HY strain. The observed phenomenon may have a universal character among fungal producers of secondary metabolites improved by classical methods, since previously the increased resistance to ODC inhibitors was also shown for Acremonium chrysogenum, a high-yielding producer of cephalosporin C.
On an SEIR Epidemic Model with Vaccination of Newborns and Periodic Impulsive Vaccination with Eventual On-Line Adapted Vaccination Strategies to the Varying Levels of the Susceptible Subpopulation
Authors: Etxeberria-Etxaniz Malen , Alonso-Quesada Santiago , De la Sen Manuel
Published: November 2020

This paper investigates a susceptible-exposed-infectious-recovered (SEIR) epidemic model with demography under two vaccination effort strategies. Firstly, the model is investigated under vaccination of newborns, which is fact in a direct action on the recruitment level of the model. Secondly, it is investigated under a periodic impulsive vaccination on the susceptible in the sense that the vaccination impulses are concentrated in practice in very short time intervals around a set of impulsive time instants subject to constant inter-vaccination periods. Both strategies can be adapted, if desired, to the time-varying levels of susceptible in the sense that the control efforts be increased as those susceptible levels increase. The model is discussed in terms of suitable properties like the positivity of the solutions, the existence and allocation of equilibrium points, and stability concerns related to the values of the basic reproduction number. It is proven that the basic reproduction number lies below unity, so that the disease-free equilibrium point is asymptotically stable for larger values of the disease transmission rates under vaccination controls compared to the case of absence of vaccination. It is also proven that the endemic equilibrium point is not reachable if the disease-free one is stable and that the disease-free equilibrium point is unstable if the reproduction number exceeds unity while the endemic equilibrium point is stable. Several numerical results are investigated for both vaccination rules with the option of adapting through ime the corresponding efforts to the levels of susceptibility. Such simulation examples are performed under parameterizations related to the current SARS-COVID 19 pandemic.
Factors of the Technology Acceptance Model for Construction IT
Authors: Park Eun Soo , Park Min Seo
Published: November 2020

The use of information technology is spreading in the construction field. However, the use of information technology in the construction field does not conform to the requirements and characteristics of users who use information technology. This fact is blindly accepted by the government and client demands, which is an impediment to the dissemination of information technology in the construction field. To improve the use of information technology in the construction field, this study analyzes the factors of acceptance of information technology according to the characteristics of users who use information technology in the construction field based on Davis’ technology acceptance model. As a result of the analysis, we found that if users consider IT in the construction industry easy to use, spontaneous attitude and behavioral intention are to be expected. Moreover, acceptance type, educational satisfaction, usage enjoyment, and usage experience are the factors that impact perceived usefulness, and educational satisfaction and usage enjoyment impact perceived ease of use as well. This study aims to derive factors that maximize the approachability and usefulness of users through the use of a technology acceptance model in construction prior to the application of new information technology in the construction field.
Design of the Cryogenic Bypass Line for the SIS100 Synchrotron
Authors: Iluk Artur , Malcher Kazimierz , Słomski Wiktor , Chorowski Maciej , Poliński Jarosław , Eisel Thomas , Streicher Branislav , Spiller Peter
Published: November 2020

This paper presents the selected aspects of a superconducting cryogenic bypass line (BPL) design, a part of the international Facility for Antiproton and Ion Research (FAIR) SIS100 cryogenic system, currently under construction in Darmstadt, Germany. Design, manufacturing, and installation of the superconducting cryogenic bypass line is a part of a Polish in-kind contribution to the FAIR project, realized by the Wroclaw University of Science and Technology. The BPL is dedicated to transferring liquid helium and AC electric current between SIS100 arc sections and superconducting quadrupole magnets located in warm straight sections of the synchrotron. A main innovative feature of the cryogenic bypass line is transferring the electric current and liquid helium in one vacuum vessel, while in other similar projects, namely, the Large Hadron Collider at CERN (CH) or the Tevatron at FermiLab (USA), those functions are separated. The coexistence of superconducting busbars and liquid helium process pipes in one limited space, as well as numerous additional functional and technical requirements, was a source of the serious design and production challenges described in the paper, including two designs of the internal suspension system based on steel rods and aramid cables.
Extending Limited In Situ Mountain Weather Observations to the Baseline Climate: A True Verification Case Study
Authors: Hofer Marlis , Horak Johannes
Published: November 2020

The availability of in situ atmospheric observations decreases with elevation and topographic complexity. Data sets based on numerical atmospheric modeling, such as reanalysis data sets, represent an alternative source of information, but they often suffer from inaccuracies, e.g., due to insufficient spatial resolution. sDoG (statistical Downscaling for Glacierized mountain environments) is a reanalysis data postprocessing tool designed to extend short-term weather station data from high mountain sites to the baseline climate. In this study, sDoG is applied to ERA-Interim predictors to produce a retrospective forecast of daily air temperature at the Vernagtbach climate monitoring site (2640 MSL) in the Central European Alps. First, sDoG is trained and cross-validated using observations from 2002 to 2012 (cross-validation period). Then, the sDoG retrospective forecast and its cross-validation-based uncertainty estimates are evaluated for the period 1979–2001 (hereafter referred to as the true evaluation period). We demonstrate the ability of sDoG to model air temperature in the true evaluation period for different temporal scales: day-to-day variations, year-to-year and season-to-season variations, and the 23-year mean seasonal cycle. sDoG adds significant value over a selection of reference data sets available for the site at different spatial resolutions, including state-of-the-art global and regional reanalysis data sets, output by a regional climate model, and an observation-based gridded product. However, we identify limitations of sDoG in modeling summer air temperature variations particularly evident in the first part of the true evaluation period. This is most probably related to changes of the microclimate around the Vernagtbach climate monitoring site that violate the stationarity assumption underlying sDoG. When comparing the performance of the considered reference data sets, we cannot demonstrate added value of the higher resolution data sets over the data sets with lower spatial resolution. For example, the global reanalyses ERA5 (31 km resolution) and ERA-Interim (80 km resolution) both clearly outperform the higher resolution data sets ERA5-Land (9 km resolution), UERRA HARMONIE (11 km resolution), and UERRA MESCAN-SURFEX (5.5 km resolution). Performance differences among ERA5 and ERA-Interim, by contrast, are comparably small. Our study highlights the importance of station-scale uncertainty assessments of atmospheric numerical model output and downscaling products for high mountain areas both for data users and model developers.
Air-Traffic Restrictions at the Madeira International Airport Due to Adverse Winds: Links to Synoptic-Scale Patterns and Orographic Effects
Authors: Belo-Pereira Margarida , Santos João A.
Published: November 2020

The Madeira International Airport (MIA) lies on the island’s south-eastern coast and it is known to be exposed to wind hazards. A link between these adverse winds at MIA and the synoptic-scale circulation is established using a weather type (WT) classification. From April to September (summer period), five WTs prevail, cumulatively representing nearly 70% of days. These WTs reflect the presence of well-established Azores high, with some variations on location and strength. Although with a low frequency of occurrence (<5%), this anticyclone occasionally strengthens and extends towards Iberia, inducing anomalously strong NNE/NE up to 3–5 km over Madeira. The most severe and longer-lasting wind conditions at the MIA, with a higher frequency of gusts above 35 kt, are driven by this synoptic-scale pattern and are more common in summer. An episode of adverse winds at the MIA is analysed, illustrating the occurrence of upstream stagnation, flow splitting, and lee wake formation. The upstream conditions include a low-level inversion, strong NNE/NE winds near and above the inversion and a Froude number less than 1. The AROME (Application of Research to Operations at Mesoscale) model predicted the occurrence of downslope winds, in association with a large-amplitude mountain wave. At this time, the strongest wind gusts were registered and one aircraft executed a missed approach. The wind regime in different places of the island suggests that these conditions are relatively frequent, mostly in summer. Finally, objective verification of AROME wind forecast, for a three-year period and from June to August, is discussed.
Spatio-Temporal Variation of Ozone Concentrations and Ozone Uptake Conditions in Forests in Western Germany
Authors: Eghdami Hanieh , Werner Willy , Büker Patrick
Published: November 2020

The study analyzes the long-term trends (1998–2019) of concentrations of the air pollutants ozone (O3) and nitrogen oxides (NOx) as well as meteorological conditions at forest sites in German midrange mountains to evaluate changes in O3 uptake conditions for trees over time at a plot scale. O3 concentrations did not show significant trends over the course of 22 years, unlike NO2 and NO, whose concentrations decreased significantly since the end of the 1990s. Temporal analyses of meteorological parameters found increasing global radiation at all sites and decreasing precipitation, vapor pressure deficit (VPD), and wind speed at most sites (temperature did not show any trend). A principal component analysis revealed strong correlations between O3 concentrations and global radiation, VPD, and temperature. Examination of the atmospheric water balance, a key parameter for O3 uptake, identified some unusually hot and dry years (2003, 2011, 2018, and 2019). With the help of a soil water model, periods of plant water stress were detected. These periods were often in synchrony with periods of elevated daytime O3 concentrations and usually occurred in mid and late summer, but occasionally also in spring and early summer. This suggests that drought protects forests against O3 uptake and that, in humid years with moderate O3 concentrations, the O3 flux was higher than in dry years with higher O3 concentrations.
Impact of VOCs and NOx on Ozone Formation in Moscow
Authors: Berezina Elena , Moiseenko Konstantin , Skorokhod Andrei , Pankratova Natalia V. , Belikov Igor , Belousov Valery , Elansky Nikolai F.
Published: November 2020

Volatile organic compounds (VOCs), ozone (O3), nitrogen oxides (NOx), carbon monoxide (CO), meteorological parameters, and total non-methane hydrocarbons (NMHC) were analyzed from simultaneous measurements at the MSU-IAP (Moscow State University—Institute of Atmospheric Physics) observational site in Moscow from 2011–2013. Seasonal and diurnal variability of the compounds was studied. The highest O3 concentration in Moscow was observed in the summer daytime periods in anticyclonic meteorological conditions under poor ventilation of the atmospheric boundary layer and high temperatures (up to 105 ppbv or 210 μg/m3). In contrast, NOx, CO, and benzene decreased from 8 a.m. to 5–6 p.m. local time (LT). The high positive correlation of daytime O3 with secondary VOCs affirmed an important role of photochemical O3 production in Moscow during the summers of 2011–2013. The summertime average concentrations of the biogenic VOCs isoprene and monoterpenes were observed to be 0.73 ppbv and 0.53 ppbv, respectively. The principal source of anthropogenic VOCs in Moscow was established to be local vehicle emissions. Yet, only about 5% of the observed isoprene was safely attributed to anthropogenic sources, suggesting significant contribution of biogenic sources into the total levels of ozone precursors. The non-linear O3–NOx dependence shows a decrease in ground-level O3 with an increase in NOx during the summers of 2011–2013, which is typical for the VOC-sensitive photochemical regime of O3 formation. Nevertheless, during the elevated ozone episodes in July 2011, the photochemical regime of ozone production was either transitional or NOx-sensitive. Contribution of various anthropogenic and biogenic VOCs into the measured ozone values was evaluated. The ozone-forming potential (OFP) of total VOCs was 31–67 μg/m3 on average and exceeded 100 μg/m3 in the top 10% of high ozone events, reaching 136 μg/m3. Acetaldehyde, 1.3-butadiene, and isoprene have the highest ozone production potential in Moscow compared to that of other measured VOCs.
Forecast Uncertainty of Rapid Intensification of Typhoon Dujuan (201521) Induced by Uncertainty in the Boundary Layer
Authors: Qin Xiaohao , Duan Wansuo
Published: November 2020

Using ensemble forecast experiments generated by the weather research and forecasting model, the forecast uncertainties of intensity and its rapid intensification (RI) induced by the uncertainty occurring in the boundary layer are investigated for Typhoon Dujuan (201521). The results show that the uncertainty in the boundary layer in the typhoon area, compared with that in other areas of the model domain, not only leads to a much larger forecast uncertainty of the typhoon intensity but also considerably perturbs the RI forecast uncertainty. Particularly, the uncertainty in the gale area in the boundary layer, compared with that in the inner-core and other areas, makes a much larger contribution to the forecast uncertainty of typhoon intensity, with the perturbations including moisture component being most strongly correlated with the occurrence of RI. Further analyses show that such perturbations increase the maximum tangential wind in the boundary layer and enhance the vorticity in the eyewall, which then facilitate the spin-up of the inner-core and induce the occurrence of RI. It is inferred that more observations, especially those associated with the moisture, should be preferentially assimilated in the gale area within the boundary layer of a tropical cyclone, which will help improve the forecast skill of the RI. These results also tell us that the boundary layer parameterization scheme should be further developed to improve the forecast skill of tropical cyclone intensity and its RI behavior.
The Temporal Evolution of PM2.5 Pollution Events in Taiwan: Clustering and the Association with Synoptic Weather
Authors: Su Shih-Hao , Chang Chiao-Wei , Chen Wei-Ting
Published: November 2020

This study conducted a cluster analysis on the fine particulate matter (PM2.5) data over Taiwan from 2006 to 2015 and diagnosed their association with the synoptic weather patterns. Five clusters are identified via a hierarchical clustering algorithm; three of them correspond to severe events, each with a distinct pattern of temporal evolution within the 240-h window. The occurrence of the different clusters exhibits strong seasonal variation. Two of the polluted clusters are more frequently associated with weak synoptic weather, while the other one is related to northeasterly winds and fronts. Detailed case studies show that the weather patterns’ temporal evolutions clearly modulate the transition among various pollution clusters by influencing the changes in local circulation and atmospheric stability. In winter, the clusters characterizing severe PM2.5 pollution events occur when Taiwan is influenced by persistent weak synoptic condition, while in autumn, the long-range transport by strong northerly winds leads to the occurrence of severe PM2.5 pollution. The current results shed light on the potential of combining the data-driven approach and the numerical weather forecasting model to provide extended range forecasts of local air pollution forecasts.