bluuebunny/crossref_metadata_2025 huggingface dataset:
PopStress: designing organizational stress intervention for office workers
Authors: Bao Yanchi , Xue Mengru , Gohumpu Jennifer , Cao Yumeng , Hu Jun
Published: December 2023

Introduction

Excessive work stress on office workers will affect people's health and work efficiency, and organizational stress management is becoming more and more critical. Current studies focus on the management of individual stress. The collective nature of stress and coping needs further exploration.

Methods

This paper proposes the PopStress system, which converts the negative stress of an office group into the energy of a popcorn machine. When the organizational stress accumulates to the threshold, the popcorn machine will start making popcorn and attract office workers to take a break and eat. Through multisensory stimuli such as visual, audio, and olfaction, the system encourages natural and entertaining social stress-relieving behaviors within the office.

Results

Twenty-four office workers were recruited and divided into six groups for the user study. The results showed that PopStress enables users to understand the collective stress status, and successfully relieved the individual's physiological and psychological stress. This work provides insights into organizational stress management, health product design, and social design.
Advancing paediatric cardiac imaging: a comprehensive analysis of the feasibility and accuracy of a novel 3D paediatric transoesophageal probe
Authors: Karsenty Clément , Hadeed Khaled , Pyra Pierrick , Guitarte Aitor , Djeddai Camelia , Vincent Remi , Dulac Yves , Silagdze Ia , Gobin Julie , Combes Nicolas , Ratsimandresy Miarisoa , Berthomieu Lionel , Calvaruso Davide , Acar Philippe
Published: December 2023

Aims

Pediatric transoesophageal echocardiography (TOE) probes have remained two-dimensional (2D) limiting their use compared to adults. While critical in pediatrics for interventions and post-surgery assessments, technological advancements introduced a three-dimensional (3D) pediatric TOE probe. This study assessed the new 3D pediatric TOE probe (GE 9VT-D) for feasibility, handling, and imaging quality.

Methods and results

At Children's Hospital of Toulouse, 2-month prospective study enrolled children undergoing TOE with the new probe. All imaging modalities were rated by 2 operators using a 5-point Likert-type scale from 1 (very poor) to 5 (very good) quality. Forty-five children, median age 3.7 (range: 2 months-14.7 years) median weight 7.8 kg (range: 4.3–48 kg) underwent 60 TOEs: 25% pre-surgery, 45% post-surgery, 28% during percutaneous procedures, and 2% in intensive care. Probe handling was “very easy” in all cases without adverse events. The median score of 2D, 2D colour, pulsed Doppler and 3D were noted 5 out of 5 and continuous Doppler and 3D colour 4 out of 5. The 3D image quality remained consistent irrespective of the patient weighing above or below 7.8 kg (p = 0.72). Postoperative TOEs identified two cases needing further interventions, emphasizing its value in evaluating surgical outcomes and also for guiding percutaneous interventions.

Conclusion

Our comprehensive evaluation demonstrates that the new 3D pediatric TOE probe is feasible and provides high-quality imaging in pediatric patients. The successful integration of this novel probe into clinical practice has the potential to enhance diagnostic accuracy and procedural planning, ultimately optimizing patient outcomes in pediatric cardiac care.
Bayesian estimation of glacier surface elevation changes from DEMs
Authors: Guillet Gregoire , Bolch Tobias
Published: December 2023

Accurate estimates of glacier surface elevation changes are paramount for various aspects of the study of the cryosphere, from glacier flow and thickness estimates to hydrological forecasts and projections of sea-level-rise. We present a novel probabilistic framework to filter outliers and estimate uncertainties in glacier surface elevation changes computed from the subtraction of digital elevation models (DEM). Our methodology frames outlier filtering as a Bayesian inference problem, thus characterizing the state of knowledge on glacier surface elevation changes through the posterior distribution as the combination of glacier volume variation observations and prior knowledge arising from previously collected data and/or modeled results. We validate this technique with experiments using Gaussian random fields to generate artificial noise in glacier surface elevation variation observations and show that the model satisfactorily culls the simulated outliers. Surface elevation change estimates are consistent with results computed from widely-used outlier filtering and uncertainty estimation techniques. The Bayesian framework allows unifying DEM error models with physical considerations on glacier surface elevation changes within a simple, statistically coherent model preventing temporal correlation and additional biases in other techniques. On the basis of these results, we discuss the implications of DEM uncertainty and offer suggestions for the glaciological community.
Correlation between visceral fat metabolism score and erectile dysfunction: a cross-sectional study from NHANES 2001-2004
Authors: Huang Lewei , Liu Heqian , Li Lianqiang , Wang Shudong , Sun Gang
Published: December 2023

Backgrounds

The factors associated with erectile dysfunction (ED) are diverse, and obesity is a significant component. Metabolic Score for Visceral Fat (METS-VF) can assess obesity more accurately than body mass index (BMI). However, the association between METS-VF and ED remains unclear.

Objective

This study aimed to investigate the association between the METS-VF and ED using National Health and Nutrition Examination Survey (NHANES) 2001-2004 data.

Methods

Data were sourced from NHANES 2001-2004. The relationship between METS-VF and ED was analyzed using multivariate logistic regression, followed by subgroup analyses to identify sensitive populations. Nonlinear correlation was evaluated through smoothed curve fitting, and a threshold effect analysis validated the findings. Comparative logistic regression of the Receiver Operating Characteristic (ROC) curve assessed the diagnostic capability of METS-VF against the classical obesity index for ED.

Results

The study enrolled 3625 participants, of whom 961 self-reported ED history and 360 reported severe ED. After adjusting for confounders, METS-VF exhibited a positive association with asthma prevalence (OR= 3.47, 95% CI: 2.83, 14.24). Stratification based on median METS-VF revealed higher ED prevalence in participants with elevated METS-VF (OR= 2.81,95% CI:2.32, 3.41). Nonlinear correlation was observed, with a significant association between METS-VF and ED when METS-VF exceeded 6.63. Subgroup analysis highlighted a stronger correlation in participants aged 50-85 years, Caucasians, hypertensive individuals, diabetics, and those with coronary heart disease. Sensitivity analysis using severe ED as the outcome reaffirmed the nonlinear positive association with METS-VF (OR=3.86, 95% CI:2.80,5.33), particularly when METS-VF surpassed 6.68.

Conclusion

Elevated METS-VF was nonlinearly correlated with increased ED incidence. Individuals with METS-VF above 6.63 should be vigilant about heightened ED risk. Special attention should be given to participants aged 50-85 years, Caucasians, hypertensive individuals, diabetics, and those with coronary heart disease.
The effect of hypothyroidism on the risk of diabetes and its microvascular complications: a Mendelian randomization study
Authors: Fang Ting , Deng Xiaoqing , Wang Jingyi , Han Fei , Liu Xiangyang , Liu Yajin , Sun Bei , Chen Liming
Published: December 2023

Context

Several observational studies have found that hypothyroidism is associated with diabetes and its microvascular complications. However, the cause and effect have not been clarified.

Objective

The aim of the study was to examine the causality of such associations by a Mendelian randomization study.

Methods

Two-sample Mendelian randomization analysis was conducted to investigate the associations. Summary statistics for hypothyroidism were from the UK Biobank, and diabetes and its microvascular complications were from the largest available genome-wide association studies. MR–Egger, weighted median, inverse variance weighted, simple mode and weighted mode were used to examine the causal associations, and several sensitivity analyses were used to assess pleiotropy.

Results

Inverse variance weighted estimates suggested that hypothyroidism was associated with type 1 diabetes and type 1 diabetes with renal complications (β= 9.059926, se= 1.762903, P = 2.76E-07 and β= 10.18375, se= 2.021879, P = 4.73E-07, respectively) but not type 2 diabetes and type 2 diabetes with renal complications. In addition, hypothyroidism was positively associated with severe nonproliferative diabetic retinopathy and proliferative diabetic retinopathy (β= 8.427943, se= 2.142493, P = 8.36E-05 and β= 3.100939, se= 0.74956, P=3.52E-05, respectively).

Conclusions

The study identified the causal roles of hypothyroidism in diabetes and its microvascular complications. Hypothyroidism can lead to type 1 diabetes, type 1 diabetes with renal complications, severe nonproliferative diabetic retinopathy and proliferative diabetic retinopathy.
Performance of halophytes in soil desalinization and its influencing factors: a meta-analysis
Authors: Wang Shiqi , Liu Jinbiao , Wang Yanyan , Liu Yalan , Tian Changyan
Published: December 2023

Soil salinization threatening natural and agricultural production challenges global food security. Halophytes are of great interest in soil desalinization in recent years; yet, there is a lack of a comprehensive quantitative overview of biotic and abiotic factors for halophytes’ desalinization performance across global scales. Here, a meta-analysis was conducted using 400 observations from 53 peer-reviewed studies to assess desalinization by halophytes in relation to 27 variables. Results showed that soil salinity was significantly decreased in halophytes field on average by 37.7% compared to control on a global scale (p < 0.05). Desalinization performance was better in cold and hot regions than in temperate regions, in dry regions than in wet regions, in alkaline saline soils than in neutral saline soils, and in conditions with low sand content than high sand content. Under aboveground harvest treatment, desalinization increased with the years of cultivation, while no trends were detected under no harvest treatment, indicating the importance of aboveground accumulation. Desalinization was not related to soil CaCO3 content but was accompanied by soil structure improvement, nutrition enrichment, and microbe propagation, implying other root-microbe-soil interactions rather than CaCO3 dissolution play important roles. Shoot biomass could be used as an indicator of the desalinization performance, and the performance would not be decreased due to the high uptake selectivity for K+ over Na+. Notably, desalinization was similar in the pot experiments and field experiments, but pot experiments would magnify the contribution of aboveground salt accumulation to desalinization. Our findings can help to expand the applicability and efficiency of halophytes for sustainable agricultural development in saline soils.
Investigating the occurrence of autoimmune diseases among children and adolescents hospitalized for Mycoplasma pneumoniae infections
Authors: Ha Eun Kyo , Kim Ju Hee , Cha Hye Ryeong , Han Bo Eun , Shin Youn Ho , Baek Hey-Sung , Choi Sun Hee , Han Man Yong
Published: December 2023

Background

Mycoplasma pneumoniae infection is common in the general population and may be followed by immune dysfunction, but links with subsequent autoimmune disease remain inconclusive.

Objective

To estimate the association of M. pneumoniae infection with the risk of subsequent autoimmune disease.

Methods

This retrospective cohort study examined the medical records of South Korean children from 01/01/2002 to 31/12/2017. The exposed cohort was identified as patients hospitalized for M. pneumoniae infection. Each exposed patient was matched with unexposed controls based on birth year and sex at a 1:10 ratio using incidence density sampling calculations. The outcome was subsequent diagnosis of autoimmune disease, and hazard ratios (HRs) were estimated with control for confounders. Further estimation was performed using hospital-based databases which were converted to a common data model (CDM) to allow comparisons of the different databases.

Results

The exposed cohort consisted of 49,937 children and the matched unexposed of 499,370 children. The median age at diagnosis of M. pneumoniae infection was 4 years (interquartile range, 2.5–6.5 years). During a mean follow-up time of 9.0 ± 3.8 years, the incidence rate of autoimmune diseases was 66.5 per 10,000 person-years (95% CI: 64.3–68.8) in the exposed cohort and 52.3 per 10,000 person-years (95% CI: 51.7–52.9) in the unexposed cohort, corresponding to an absolute rate of difference of 14.3 per 10,000 person-years (95% CI: 11.9–16.6). Children in the exposed cohort had an increased risk of autoimmune disease (HR: 1.26; 95% CI: 1.21–1.31), and this association was similar in the separate analysis of hospital databases (HR: 1.25; 95% CI 1.06–1.49).

Conclusion

M. pneumoniae infection requiring hospitalization may be associated with an increase in subsequent diagnoses of autoimmune diseases.
Wound healing and Cadmium detoxification in the earthworm Lumbricus terrestris – a potential case for coelomocytes?
Authors: Aigner Gerhard P. , Peer Veronika , Fiechtner Birgit , Piechnik Cláudio Adriano , Höckner Martina
Published: December 2023

Earthworms are affected by physical stress, like injury, and by exposure to xenobiotics, such as the toxic metal cadmium (Cd), which enters the environment mainly through industry and agriculture. The stress response to the single and the combination of both stressors was examined in regenerative and unharmed tissue of Lumbricus terrestris to reveal if the stress response to a natural insult like injury (amputation) interferes with Cd detoxification mechanisms. We characterized the roles of metallothionein 1 (MT1) and MT2 isoforms, heat shock protein 70 as well as immune biomarkers such as the toll-like receptors (TLR) single cysteine cluster TLR and multiple cysteine cluster TLR. The role of the activated transcription factors (ATFs) ATF2, ATF7, and the cAMP responsive element binding protein as putative regulatory intersection as well as a stress-dependent change of the essential trace elements zinc and calcium was analyzed. Phosphorylated AMP activated protein kinase, the cellular energy sensor, was measured to explore the energy demand, while the energy status was determined by detecting carbohydrate and protein levels. Taken together, we were able to show that injury rather than Cd is the driving force that separates the four treatment groups – Control, Cd exposure, Injury, Cd exposure and injury. Interestingly, we found that gene expression differed regarding the tissue section that was analyzed and we hypothesize that this is due to the migration of coelomocytes, earthworm immune cells, that take over a key role in protecting the organism from a variety of environmental challenges. Surprisingly, we discovered a role for MT1 in the response to multiple stressors and an isoform-specific function for the two newly characterized TLRs. In conclusion, we gathered novel information on the relation of innate immunity, wound healing, and Cd detoxification mechanisms in earthworms.
Human neutrophil extracellular traps do not impair in vitro Toxoplasma gondii infection
Authors: Macedo Isabela S. , Lara Flávio A. , Barbosa Helene S. , Saraiva Elvira M. , Menna-Barreto Rubem F. S. , Mariante Rafael M.
Published: December 2023

Introduction

Toxoplasma gondii, responsible for causing toxoplasmosis, is a prevalent food and waterborne pathogen worldwide. It commonly infects warm-blooded animals and affects more than a third of the global human population. Once ingested, the parasite enters the host’s small intestine and rapidly disseminates throughout the body via the bloodstream, infiltrating various tissues. Leukocyte-driven responses are vital against T. gondii, with neutrophils playing a dual role: swiftly recruited to infection sites, releasing inflammatory mediators, and serving as a replication hub and Trojan horses, aiding parasite spread. Neutrophils from various hosts release extracellular traps (NETs) against the protozoan. However, gaps persist regarding the mechanisms of NETs production to parasite and their significance in infection control. This study investigates the interplay between human neutrophils and T. gondii, exploring dynamics, key molecules, and signaling pathways involved in NETs production upon protozoan challenge.

Methods and Results

Using confocal and electron microscopy, live cell imaging, pharmacological inhibitors, and DNA quantification assays, we find that human neutrophils promptly release both classical and rapid NETs upon pathogen stimulation. The NETs structure exhibits diverse phenotypes over time and is consistently associated with microorganisms. Mechanisms involve neutrophil elastase and peptidylarginine deiminase, along with intracellular calcium signaling and the PI3K pathway. Unexpectedly, human traps do not diminish viability or infectivity, but potentially aid in capturing parasites for subsequent neutrophil phagocytosis and elimination.

Discussion

By revealing NETs formation mechanisms and their nuanced impact on T. gondii infection dynamics, our findings contribute to broader insights into host-pathogen relationships.
Biology of endophilin and it’s role in disease
Authors: Yang Lu-Qi , Huang An-Fang , Xu Wang-Dong
Published: December 2023

Endophilin is an evolutionarily conserved family of protein that involves in a range of intracellular membrane dynamics. This family consists of five isoforms, which are distributed in various tissues. Recent studies have shown that Endophilin regulates diseases pathogenesis, including neurodegenerative diseases, tumors, cardiovascular diseases, and autoimmune diseases. In vivo, it regulates different biological functions such as vesicle endocytosis, mitochondrial morphological changes, apoptosis and autophagosome formation. Functional studies confirmed the role of Endophilin in development and progression of these diseases. In this study, we have comprehensively discussed the complex function of Endophilin and how the family contributes to diseases development. It is hoped that this study will provide new ideas for targeting Endophilin in diseases.
Experimental study on the effect of sound stimulation on hearing and behavior of juvenile black rockfish (Sebastes schlegelii)
Authors: Wang Yining , Huang Liuyi , Xing Binbin
Published: December 2023

Assessing the potential impacts of wind farm noise on fish is a crucial aspect of Environmental Impact Assessment (EIA) studies. There is increasing evidence of disturbances and effects on hearing and behavior in animals. The black rockfish (Sebastes schlegelii) is a commercially valuable rocky reef fish native to East Asia. However, empirical studies that measure the actual consequences are lacking. In this study, we used auditory evoked potentials (AEP) to assess the effects of dominant frequency noise emitted by offshore wind farms on the auditory sensitivity, hearing threshold, swimming, and feeding behavior of juvenile black rockfish. The experimental findings revealed that the most sensitive sound frequency was 200 Hz, with the lowest hearing threshold recorded at 86.4 ± 3.4 dB re 1 μPa. Following 3 and 7 days of exposure to 200 Hz noise at 110 dB, threshold shifts in black rockfish reached 19.0 dB and 13.3 dB, respectively. During the subsequent recovery phase, these shifts decreased to approximately 9.8 dB after 3 days, respectively. The noise-exposed group exhibited higher swimming duration, moving distance, and caudal fin swing frequency compared to the control group without noise exposure. Furthermore, noise prolonged the feeding rate of black rockfish. Our findings provide the first evidence of noise-induced temporary threshold shift and behavioral disturbances in juvenile black rockfish, implying potential fitness consequences associated with noise pollutant.
Fe mediated alleviation effect of cadmium toxicity in mangrove Avicennia marina (Forssk.) Vierh
Authors: Li Jian , Wu Yueming , Guo Xiaotong , Jiang Shan , Leng Zhanrui , Xia Jiaojiao , Zhuo Chongyu , Jia Hui , Du Daolin
Published: December 2023

Iron (Fe) supply is one of the key factors in alleviating cadmium (Cd) stress in different plants, including mangroves. However, the effects and mechanisms of Fe in mediating the mangrove response to Cd stress still need to elaborate on the level of plant physiology and metabolism. Herein a 40-day hydroponic study was conducted to evaluate the mediating effect of Fe on Cd tolerance of Avicennia marina (Forssk.) Vierh. Results showed that the Fe supply directly improved the activity of ferric chelate reductase (FCR), increased Fe concentration in tissues, enhanced its competitiveness with Cd, and improved Fe bioavailability, thereby reducing the uptake of Cd by plants. Simultaneously, moderate Fe enhanced the photosynthesis and increased concentrations of total phenolics and tannins in both roots and leaves that play a critical role in chelating and immobilizing Cd, thus restricting the Cd translocation from roots to aboveground tissues. Additionally, the application of Fe significantly upregulated the activity of antioxidant enzymes, including POD and PPO and hence scavenging the reactive oxygen species caused by Cd stress. In conclusion, moderate Fe application improved the growth of A. marina and enhanced its tolerance to Cd at physiological and metabolic levels. Therefore, improving Fe bioavailability will reduce the mangrove degradation caused by Cd contamination, and also this may be a potential way to restore contaminated mangrove ecosystems. Protecting and restoring mangroves is not only essential for preserving biodiversity and supporting coastal ecosystems but also crucial for maintaining their role as crucial carbon sinks (i.e., mangrove blue carbon) combating global warming and climate change.
Geographic origin and host’s phylogeny are predictors of the gut mucosal microbiota diversity and composition in Mediterranean scorpionfishes (Scorpaena spp.)
Authors: Lilli Ginevra , Sirot Charlotte , Campbell Hayley , Brophy Deirdre , Graham Conor T. , George Isabelle F.
Published: December 2023

The gut microbiome holds an important role in the health and homeostasis of fishes. However, despite the large diversity and distribution of this vertebrate group, only the intestinal microbiome of a limited number of freshwater and marine fish species has been well characterized to date. In this study, we characterize the gut mucosal microbial communities of three commercially valuable Scorpaena spp. (n=125) by using a comprehensive comparative dataset including 16S rRNA gene amplicon data from four different locations in the Mediterranean Sea. We report that the geographical origin of the individuals influences the diversity and the composition of the gut microbial communities more than the host’s phylogenetic relatedness in this fish group. Moreover, we observe a positive correlation between the composition of the gut microbiota and the phylogenetic distance between the hosts (i.e. phylosymbiosis). Finally, the core microbiota of each species is described both regionally and across the Mediterranean Sea. Only a few bacterial genera appear to be residents of the scorpionfishes’ gut microbiota across the Mediterranean Sea: Photobacterium, Enterovibrio, Vibrio, Shewanella, Epulopiscium, Clostridium sensu stricto 1 and Rombutsia in S. notata, Clostridium sensu stricto 1, Cetobacterium and Rombutsia in S. porcus, and only Clostridium sensu stricto 1 in S. scrofa. This study highlights the importance of investigating the gut microbiome across a species’ geographical range and it suggests this as a general procedure to better characterize the gut microbial ecology of each fish species.
Durability of geopolymer stabilized domestic waste incineration slag blending macadam in pavement base
Authors: Ji Xiaoping , Mou Keliang , Zhang Ting , Dong Xinze
Published: December 2023

In order to achieve the high quality and large-scale utilization of domestic waste incineration slag (WIS) in pavement base, a geopolymer prepared using fly ash from domestic waste incineration was used to replace the cement to stabilized WIS blending macadam used in the pavement base. The durability of the geopolymer stabilized WIS blending macadam (called as GeoWIS) was investigated, including the water stability, freeze-thaw resistance and dry shrinkage. The strength formation was identified using SEM analyses. Furthermore, the durability of GeoWIS was investigated and compared with those of cement stabilized macadam (CSM). Results show that the 7 days compressive strength of GeoWIS meets the strength criterion of the asphalt pavement base (≥2 MPa), except for the combination of 8% geopolymer and 100% WIS. The water stability coefficient and residual compressive strength ratio of GeoWIS increase as the geopolymer increases and WIS decreases. The cumulative dry shrinkage and dry shrinkage coefficient decrease as the WIS increases, while the cumulative water loss rate of GeoWIS increases. SEM analysis shows that the geopolymer reaction generates C-S-H gels, N-A-S-H gels, and Aft, which is the source of the GeoWIS strengths. GeoWIS has better water stability than CSM, and its freeze-thaw resistance and average dry shrinkage coefficient are lower than the average values for CSM.
Virtual reality in managing dental pain and anxiety: a comprehensive review
Authors: Fan Lin , Zeng Jie , Ran Longkuan , Zhang Chao , Wang Jing , Yu Cong , Zhao Nan
Published: December 2023

Objectives

This study aimed to identify, analyze, and summarize the clinical efficacy of virtual reality (VR) distraction therapy for oral treatment in different hospital settings in contrast to medical interventions that induce anxiety and pain. Furthermore, this review aimed to determine the implications for research and clinical practice of VR distraction therapy.

Data

This review investigated the clinical efficacy of VR in the oral treatment of procedural pain or anxiety. Quality assessment of the included studies was conducted. A narrative synthesis of the collected data was performed.

Sources

Literature studies from six electronic databases were searched for a comprehensive review, namely, the Cochrane Oral Health’s Trials Register, Cochrane Central Register of Controlled Trials (Central), MEDLINE (PubMed), EMBASE, Scopus, and Web of Science.

Study selection

One thousand five hundred twenty-two patients aged between 0 and 60 years who used VR during dental treatment were included in this review. Among these studies, 8 and 14 studies comprised adult and pediatric patients.

Conclusion

Overall, the reviewed studies underscore the efficacy of VR to mitigate pain and anxiety in the context of dental treatment. VR is an innovative pain and anxiety management approach that facilitates dental treatment patients to immerse themselves in a virtual world while using distractions to reduce pain and anxiety.

Clinical significance

VR is an effective and novel non-pharmacological method of behavioral management that contributes to improving medication safety for dental patients. VR as a distractive approach can reduce the fear associated with medical interventions and prevent severe pain sensitivity, anxiety, and medical avoidance among adults and children.
The extracellular polysaccharide determine the physico-chemical surface properties of Microcystis
Authors: Yang Haijian , Wu Denghua , Li Hua , Hu Chunxiang
Published: December 2023

Microcystis possesses the capacity to form colonies and blooms in lakes and reservoirs worldwide, causing significant ecological challenges in aquatic ecosystems. However, little is known about the determining factors of physico-chemical surface properties that govern the competitive advantage of Microcystis. Here, The physico-chemical surface properties of Microcystis wesenbergii and Microcystis aeruginosa, including specific surface area (SSA), hydrophobicity, zeta potential, and functional groups were investigated. Additionally, the extracellular polysaccharide (EPS) were analyzed. Laboratory-cultured Microcystis exhibited hydrophilic, a negative zeta potential and negatively charged. Furthermore, no significant relationship was shown between these properties and the cultivation stage. Microcystis wesenbergii exhibited low free energy of cohesion, high surface free energy, high growth rate, and high EPS content during the logarithmic phase. On the other hand, M. aeruginosa displayed lower free energy of cohesion, high surface free energy, high EPS content, and high growth rate during the stationary phase. These characteristics contribute to their respective competitive advantage. Furthermore, the relationship between EPS and surface properties was investigated. The polysaccharide component of EPS primarily influenced the SSA and total surface energy of Microcystis. Likewise, the protein component of EPS influenced hydrophobicity and surface tension. The polysaccharide composition, including glucuronic acid, xylose, and fructose, mainly influenced surface properties. Additionally, hydrophilic groups such as O–H and P–O–P played a crucial role in determining hydrophobicity in Microcystis. This study elucidates that EPS influenced the SSA, hydrophobicity, and surface free energy of Microcystis cells, which in turn impact the formation of Microcystis blooms and the collection.
Percutaneous ballon compression for recurrent TN —a retrospective study of 33 cases
Authors: Feng Dongwei , Zhang Yaxin , Li Dong , Wang Kang , Yang Fan , Ding Jianan , Wu Weize , Wang Yunhe , Jia Heping
Published: December 2023

Objective

To investigate the clinical efficacy of percutaneous microballoon compression in the treatment of recurrent TN.

Methods

This retrospective study included 33 patients who underwent percutaneous microballoon compression for the treatment of recurrent TN from March 2019 to May 2022. Postoperative pain recurrence and facial numbness were assessed according to the Barrow Neurological Institute (BNI) pain score. Patients’ anxiety and sleep status during follow-up were assessed according to the Self-rating Anxiety Scale (SAS) and Pittsburgh Sleep Quality Index (PSQI).

Results

All patients (33 cases) were followed up for 12–38 months, with an average follow-up time of 23 months. On postoperative day 1, 31 patients (93.9%) reported no pain, and 2 patients were given drug treatment for pain relief, The total efficacy was 93.9%. Moreover, 2 patients (6.1%) reported significant pain relief 2 weeks postoperatively. There are many complications during and after PBC. The incidence of the trigeminocardiac reflex (TCR) during surgery was 100%, and the incidence of facial numbness, masseter muscle weakness, labial herpes and headache was 97, 60.6, 12.1 and 3%. No patient experienced severe facial numbness, hearing impairment, diplopia, injury to cranial nerves, Meningitis, intracranial haemorrhage or keratitis. 1 patient had recurrence of pain at 6 months post-op, which was relieved by oral medication. 81.8% suffered from anxiety and 54.5% had poor sleep quality before surgery. After the period of PBC, SAS and PSQI scores decreased continuously. There were significant improvements in anxiety and sleep status postoperatively compared with preoperatively.

Conclusion

PBC is a safe and effective option for the treatment of recurrent TN. The arduous and demanding nature of the clinical course subjects the patient to severe pain, mental, and physical stress. Thankfully, it significantly improves the symptoms of anxiety, depression, and sleep quality.
Protocol optimization and reducing dropout in online research
Authors: Staggs Halee , Mills-Finnerty Colleen
Published: December 2023

Online research has advantages over in-person research; it’s cost-efficient, scalable, and may increase diversity. Researchers collecting data online can assess protocol performance with classification models like a decision tree. However, challenges include attrition, lack of testing environment controls, technical limitations, and lack of face-to-face rapport and real time feedback. It is necessary to consider human factors of the teleresearch process from recruitment to data collection. Here we document the impact of protocol optimizations on social media engagement and retention between a pilot sample of Veterans (n = 26) and a post-optimization sample of both Veterans and civilians (n = 220) recruited from Facebook advertisements. Two-sided tests for equality of proportions were statistically significant: advertisement views leading to clicks increased by 23.8% [X2(1) = 130.3, p < 0.001] and completion of behavioral tasks increased by 31.2% [X2(1) = 20.74, p < 0.001]. However, a proportion of participants dropped out of the study before completion for both samples. To explore why, a C5.0 decision tree was used to find features that classify participant dropout. The features chosen by the algorithm were nicotine use (100%) and cannabis use (25.6%). However, for those completing the study, data quality of cognitive performance was similar for users and nonusers. Rather than determining eligibility, participants who endorse using nicotine, or both nicotine and cannabis, may have individual differences that require support in online protocols to reduce drop out, such as extra breaks. An introduction page that humanizes participants’ lifestyle habits as a naturalistic benefit of remote research may also be helpful. Strategies are discussed to increase engagement and improve data quality. The findings have implications for the feasibility of conducting remote research, an increasingly popular approach that has distinct challenges compared to in-person studies.
The impact of characters like Tony the Tiger and other child-targeted techniques used in food and beverage marketing
Authors: Mulligan Christine , Remedios Lauren , Ramsay Tim , Pauzé Elise , Bagnato Mariangela , Potvin Kent Monique
Published: December 2023

Introduction

Food marketing’s impact is a function of exposure and power, both of which contribute to children’s poor diet quality and obesity risk. Children’s exposure to food marketing is well documented, however, few studies have assessed the impact of specific persuasive marketing techniques or aspects of ‘power’ on children.

Methods

This study administered an online survey to 1,341 Canadian children (9–12 years) aiming to determine the impact of: (1) child-targeted vs. adult-targeted marketing, and (2) licensed characters vs. spokes characters on children’s food preferences and behavioral intentions. Participants were randomized to a single condition in each survey part and viewed 3 static food advertisements displaying the features of that condition (e.g., child-targeted advertising or licensed characters), and answered 3 Likert-scale (5-point) questions after each exposure. For each condition within each research question, there were four outcome variables related to the impact of marketing on children: food preference, purchase intent, pester power, and total impact. ANOVA tested the difference in impact (Likert scores) between conditions overall and for each outcome, with Bonferroni post-hoc tests where necessary.

Results

A greater average total impact was observed among children exposed to child-targeted ads (mean Likert score 3.36) vs. adult-targeted ads (mean score 2.75; p < 0.001) or no marketing (mean score 2.81; p < 0.001). Children exposed to ads featuring spokes characters had a higher average total impact (mean score 3.98) vs. licensed characters (mean score 3.80; p < 0.001) and the control (i.e., no characters) (mean score 3.19; p < 0.001), and the total impact of licensed characters was greater than that of no characters. Similar trends were observed for all other outcomes.

Discussion

Overall, this study showed that child-targeted ads and those using characters - especially spokes characters - have a strong overall impact on children’s food preferences, purchase intents, and pester power, and support the implementation of comprehensive marketing restrictions to protect children.
Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling
Authors: Pejšková Lucie , Rønning Sissel Beate , Kent Matthew Peter , Solberg Nina Therese , Høst Vibeke , Thu-Hien To , Wold Jens Petter , Lunde Marianne , Mosleth Ellen , Pisconti Addolorata , Kolset Svein Olav , Carlson Cathrine Rein , Pedersen Mona Elisabeth
Published: December 2023

Introduction: The skeletal muscle deformity of commercial chickens (Gallus gallus), known as the wooden breast (WB), is associated with fibrotic myopathy of unknown etiology. For future breeding strategies and genetic improvements, it is essential to identify the molecular mechanisms underlying the phenotype. The pathophysiological hallmarks of WB include severe skeletal muscle fibrosis, inflammation, myofiber necrosis, and multifocal degeneration of muscle tissue. The transmembrane proteoglycans syndecans have a wide spectrum of biological functions and are master regulators of tissue homeostasis. They are upregulated and shed (cleaved) as a regulatory mechanism during tissue repair and regeneration. During the last decades, it has become clear that the syndecan family also has critical functions in skeletal muscle growth, however, their potential involvement in WB pathogenesis is unknown.

Methods: In this study, we have categorized four groups of WB myopathy in broiler chickens and performed a comprehensive characterization of the molecular and histological profiles of two of them, with a special focus on the role of the syndecans and remodeling of the extracellular matrix (ECM).

Results and discussion: Our findings reveal differential expression and shedding of the four syndecan family members and increased matrix metalloproteinase activity. Additionally, we identified alterations in key signaling pathways such as MAPK, AKT, and Wnt. Our work provides novel insights into a deeper understanding of WB pathogenesis and suggests potential therapeutic targets for this condition.
Barriers to HPV vaccination in marginalized Roma communities in Slovakia
Authors: Filakovska Bobakova Daniela , Plavnicka Jana , Urbancikova Ingrid , Edelstein Michael , Jansen Danielle , Dankulincova Veselska Zuzana
Published: December 2023

Introduction

Limited access to healthcare services leads to lower vaccination rates in marginalized Roma communities (MRCs). This study aimed to explore health system barriers to HPV vaccination faced by people from MRCs from multiple perspectives.

Methods

The qualitative study was conducted in Slovakia in 2021/22 with 43 community members and health professionals. Data were analyzed using a combination of content analysis and consensual qualitative research.

Results

A substantial barrier to vaccination is limited coverage of vaccination expenses for certain age categories by health insurance. Moreover, Slovakia faces a significant shortage of healthcare personnel, leading to work overload and a lack of capacity and motivation to address HPV vaccination. Impaired relationships between health care providers and people from MRCs lead to the avoidance of healthcare services, which contributes to insufficient delivery of information and a lack of awareness regarding HPV-related diseases and vaccination.

Conclusion

Strengthening the capacities of health care providers, expanding the age group covered by health insurance and providing tailored information to people from MRCs are necessary prerequisites to increase the availability of HPV vaccination and enable people to make informed decisions about HPV vaccination.
Frailty and medication adherence among older adult patients with hypertension: a moderated mediation model
Authors: Wang Anshi , Wan Jingjing , Zhu Lijun , Chang Weiwei , Wen Liying , Tao Xiubin , Jin Yuelong
Published: December 2023

Objective

Medication adherence has a critical impact on the well-being of older adult patients with hypertension. As such, the current study aimed to investigate the mediating role of health literacy between frailty and medication adherence and the moderating role of educational level.

Methods

This cross-sectional study included patients admitted to the geriatric unit of a hospital. Participants were interviewed using the four-item Morisky Medication Adherence Scale, the Frailty Phenotype Scale, and the Health Literacy Management Scale. Spearman’s correlation coefficients were used to assess the association between variables. Mediation and moderated mediation analyses were performed using Process version 4.1 via Model 4 and 14, respectively.

Results

Data from 388 participants were analyzed. The median (IQR [P25–P75]) score for medication adherence was 4.00 (2.00–4.00). Results revealed that after controlling for age, sex, hypertension complication(s) and body mass index, frailty significantly contributed to medication adherence (βtotal −0.236 [95% confidence interval (CI) −0.333 to −0.140]). Medication adherence was influenced by frailty (βdirect −0.192 [95% CI −0.284 to −0.099]) both directly and indirectly through health literacy (βindirect −0.044 [95% CI −0.077 to −0.014]). Educational level moderated the pathway mediated by health literacy; more specifically, the conditional indirect effect between frailty and medication adherence was significant among older adult hypertensive patients with low, intermediate, and high educational levels (effect −0.052 [95% CI −0.092 to −0.106]; effect −0.041 [95% CI −0.071 to −0.012]; effect −0.026 [95% CI −0.051 to −0.006]). The relationship between frailty and medication adherence in older adult patients with hypertension was found to have mediating and moderating effects.

Conclusion

A moderated mediation model was proposed to investigate the effect of frailty on medication adherence. It was effective in strengthening medication adherence by improving health literacy and reducing frailty. More attention needs to be devoted to older adult patients with hypertension and low educational levels.
The impact of hostile abortion legislation on the United States maternal mortality crisis: a call for increased abortion education
Authors: Kheyfets Anna , Dhaurali Shubhecchha , Feyock Paige , Khan Farinaz , Lockley April , Miller Brenna , Cohen Lauren , Anwar Eimaan , Amutah-Onukagha Ndidiamaka
Published: December 2023

The growing restrictive abortion policies nationwide and the Supreme Court decision on Dobbs v. Jackson Women’s Health Organization place increasing barriers to abortion access in the United States. These restrictions disproportionately affect low-income people of color, immigrants, and non-English speakers, and have the potential to exacerbate already existing racial inequities in maternal and neonatal outcomes. The United States is facing a Black maternal health crisis where Black birthing people are more than twice as likely to experience maternal mortality and severe maternal morbidity compared to White birthing people. Restrictions creating geographic, transportation, and financial barriers to obtaining an abortion can result in increased rates of maternal death and adverse outcomes across all groups but especially among Black birthing people. Restrictive abortion laws in certain states will decrease already limited training opportunities in abortion care for medical professionals, despite the existing abortion provider shortage. There is an immediate need for federal legislation codifying broad abortion care access into law and expanding access to abortion training across medical education. This commentary explores the impact of restrictive abortion laws on the Black maternal health crisis through multiple pathways in a logic model. By identifying current barriers to abortion education in medical school and residency, we created a list of action items to expand abortion education and access.
A brief insight into Citrobacter species - a growing threat to public health
Authors: Jabeen Ishrat , Islam Sohidul , Hassan A. K. M. Imrul , Tasnim Zerin , Shuvo Sabbir R.
Published: December 2023

Citrobacter spp. are Gram-negative, non-spore forming, rod-shaped, facultative anaerobic bacteria from the Enterobacteriaceae family often found in soil, sewage, sludge, water, food, and the intestinal tracts of animals and humans. Several members of Citrobacter spp. especially C. freundii, C. koseri, C. braakii are frequently detected in newborn illnesses, urinary tract infections, and patients with severe underlying conditions, including hypertension, diabetes, cancer, and respiratory infections, or those who are immunocompromised. Strains of Citrobacter spp. can spread vertically or horizontally from carriers or other hospital sources and thus cause nosocomial infections in hospital settings. A total of 19 Citrobacter genomospecies have been recognized based on genomics. It has been noted that the Citrobacter genus acquired antimicrobial resistance and virulence, including invasion, colonization, biofilm formation, and toxin production. The recent emergence and spread of antimicrobial resistance to β-lactams, carbapenems, fluoroquinolones, aminoglycosides, and colistin in Citrobacter spp. through chromosomal and plasmid-mediated resistance limits the empiric treatment options. Therefore, combination therapy involving costly and potentially hazardous antibiotics poses significant challenges in treating Citrobacter infections. Here we summarized the nomenclature of Citrobacter spp., clinical manifestations, epidemiology, pathogenesis, antibiotic resistance mechanisms, and treatments from various clinical samples. This review will expand our knowledge of the genomics and epidemiology of Citrobacter spp., enabling improved control of infections and the spread of these organisms.
New Insight into Organomineral Interactions in Soils. The Impact of Clay-Size Peat-Derived Organic Species on the Structure and the Strength of Soil Silt Aggregates
Authors: Skic Kamil , Adamczuk Agnieszka , Boguta Patrycja , Gryta Angelika , Masoudi Soltani Salman , Ignatova Svetlana , Józefaciuk Grzegorz
Published: December 2023

Knowledge of the effects of different organic species on soil structure and strength is gained mostly from experiments on natural soils amended with organic substances of various particle sizes, pH, ionic composition, and inorganic impurities. It greatly diversifies the experimental results and shadows individual effects of organic amendments. Therefore, to look for a clearer view, we examined the impact of HCl-washed clay-size organic species: peat, humic acids, residue after humic acid extraction, and two biochars, all derived from the same peat and having similar particles, on the structure and strength of artificial soil silt aggregates using mercury intrusion porosimetry, bulk density measurements, SEM, and uniaxial compression. Bulk density increased due to humic acid addition and decreased for the other amendments. The total pore volumes behaved oppositely. All organic substances except humic acid decreased the pore surface fractal dimension, indicating a smoothening of the pore surface. Humic acid appeared to occupy mostly the spaces between the silt grains skeleton, while the other species were also located upon silt grains. The latter effect was most evident for 600 °C heated biochar. Humic acid, peat, and the residue after humic acid extraction improved mechanical stability, whereas both biochars weakened the aggregates, which means that bulk density plays a smaller role in the mechanical stability of granular materials, as it is usually considered. A new equation relating maximum stress and the amount of the organic additives was proposed.