bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Paleoreef Reservoir Characterization through Machine Learning and Multi-Attribute Seismic Analysis: Silurian Reef Examples from the Michigan Basin
Authors: Buist Carl , Bedle Heather , Rine Matthew , Pigott John
Published: March 2021

Historically, Silurian pinnacle reef complexes in the Michigan Basin have been largely identified using 2D seismic with very little research on the reservoir characterization of these reefs using 3D seismic data. By incorporating a high-resolution 3D dataset constrained by a well-studied and data-rich paleoreef reservoir, the Puttygut reef, seismic attributes were correlated to petrophysical properties through machine learning and self-organizing maps (SOMs). A suite of structural and frequency-based attributes was calculated from pre-stack time migrated (PSTM) seismic data, with only a subset of them selected as SOM inputs. Structural attributes enhanced details in the reef but frequency attributes were overall more useful for correlating with reservoir quality. A strong relationship between certain combination percentages of attributes and certain sections of the reef with porosity and permeability was found after the SOM results were compared to wireline log and core analysis data. Areas with high permeability and porosity correlated with the average frequency and spectral decomposition at 29 and 81 Hz. Areas with high porosity and varying permeability correlated with the average frequency and spectral decomposition at 29, 57, and 81 Hz. Areas with intermediate porosity correlated with the average frequency and spectral decomposition at 29 and 57 Hz. The efficacy of the procedure was then demonstrated on two nearby reefs with very similar results.
ATP Bioluminescence for Assessing the Efficacy of the Manual Cleaning Procedure during the Reprocessing of Reusable Surgical Instruments
Authors: Masia Maria Dolores , Dettori Marco , Deriu Grazia Maria , Bellu Sabina , Arcadu Lisa , Azara Antonio , Piana Andrea , Palmieri Alessandra , Arghittu Antonella , Castiglia Paolo
Published: March 2021

Achieving sterilization by adopting proper practices is essential to ensure that surgical instruments do not transmit microorganisms to patients. As the effectiveness of sterilization mandates effective cleaning, it is necessary to verify the success of cleaning procedures. In this study, we used the adenosine triphosphate (ATP) bioluminescence method for assessing the efficacy of the manual cleaning procedure during the reprocessing of reusable surgical instruments. The ATP bioluminescence assay was performed on 140 surgical instruments of 12 different types, both before being cleaned (baseline) and after each of the cleaning procedures (i.e., decontamination, manual washing, drying, and visual inspection). For each instrument, two swabs were used as follows: one to sample the entire surface (test point 1) and the other to sample the most difficult part of the surface to clean (test point 2). Overall, for each type of instrument, there was a decrease in contamination ranging from 99.6 to >99.9% (log reduction from 2.40 to 3.76). Thus, in order to standardize the assessment of cleanliness, it may be useful to introduce the bioluminescence method into the daily routine or, at least, at regular time intervals as a complementary check combined with visual inspection. This would allow real-time verification of the achievement of an adequate level of cleanliness.
Factors Related to Blood Pressure Response after Community-Based Exercise Program in the Elderly Population
Authors: Wang Yi-Pin , Tseng Kuo-Wei , Lin Meng-Hui , Tsai Mei-Wun
Published: March 2021

Exercise has been recommended for blood pressure (BP) control, but not every individual can improve BP and reduce the risk of cardiovascular disease effectively by exercise. This study aimed to evaluate the BP response after 12-week exercise intervention and then identify the potential factors of responders on BP (R-BP) control. This was a retrospective cohort study from a project of Taipei City Government. Subjects completed the original program were included for further analysis. Sociodemographic factors, health-related behaviors, and cardiovascular risks were extracted as potential factors. The results were categorized into R-BP control, i.e., BP under optimal level (systolic BP (SBP) < 140 mmHg; and diastolic BP (DBP) < 90 mmHg) or a significant BP reduction (SBP ↓10 mmHg or DBP ↓5 mmHg) after intervention, or non-responder on BP control, i.e., subjects who failed to achieve the targets. There were 81.62% R-BP subjects. R-BP showed lower SBP and lower risk of hypertension at baseline. Active lifestyle could quadruple the number of R-BP. Higher educational level or more prescription medications were likely to be R-BP in subjects with diagnosed hypertension. Active lifestyle combined with exercise could benefit R-BP in the elderly population. Health-related factors also need to be considered for BP control.
Competitive Revenue Strategies in the Medical Consumables Industry: Evidence from Human Resources, Research and Development Expenses and Industry Life Cycle
Authors: Chen Jianxiong , Yang Chung-Cheng
Published: March 2021

This study attempted to explore the competitive advantage strategies of the medical consumables industry (MCI) from the perspectives of human resources, research and development (R&D) and the industry life cycle. As one of the essential branches of modern medical device industry, the MCI has developed rapidly in recent years as global demand for medical consumables has shown continual growth, but it also faces market uncertainty. This study took Taiwan’s small/medium medical consumables enterprises (SMMCEs) as a sample, and used the translog revenue function to study the competitive advantage of the MCI through human resource and R&D investment strategies and the stage characteristics of the industry life cycle curve. The results showed that the various human resources and R&D expenses of the small/medium medical consumables industry (SMMCI) can interact with each other to influence total revenue and that the SMMCI needs more varied types of human resources to enhance its competitive advantage. The total revenue of the SMMCI decreased as education inputs rose, but it increased along with increases in the number of employee and R&D inputs. Observed from the life cycle curve of the SMMCI, total revenue increased rapidly during the startup and growth stages, increased slowly during the maturity stage, and decreased during the decline stage. Finally, we put forward competitive advantage strategies and management suggestions for medical consumables enterprises (MCEs). We are the first to document the life cycle curve and competitive advantage strategies of the MCI, thereby contributing to the related literature.
Putative LysM Effectors Contribute to Fungal Lifestyle
Authors: Suarez-Fernandez Marta , Aragon-Perez Ana , Lopez-Llorca Luis Vicente , Lopez-Moya Federico
Published: March 2021

Fungal LysM effector proteins can dampen plant host–defence responses, protecting hyphae from plant chitinases, but little is known on these effectors from nonpathogenic fungal endophytes. We found four putative LysM effectors in the genome of the endophytic nematophagous fungus Pochonia chlamydosporia (Pc123). All four genes encoding putative LysM effectors are expressed constitutively by the fungus. Additionally, the gene encoding Lys1—the smallest one—is the most expressed in banana roots colonised by the fungus. Pc123 Lys1, 2 and 4 display high homology with those of other strains of the fungus and phylogenetically close entomopathogenic fungi. However, Pc123 Lys3 displays low homology with other fungi, but some similarities are found in saprophytes. This suggests evolutionary divergence in Pc123 LysM effectors. Additionally, molecular docking shows that the NAcGl binding sites of Pc123 Lys 2, 3 and 4 are adjacent to an alpha helix. Putative LysM effectors from fungal endophytes, such as Pc123, differ from those of plant pathogenic fungi. LysM motifs from endophytic fungi show clear conservation of cysteines in Positions 13, 51 and 63, unlike those of plant pathogens. LysM effectors could therefore be associated with the lifestyle of a fungus and give us a clue of how organisms could behave in different environments.
Recent Highlights of Research on miRNAs as Early Potential Biomarkers for Cardiovascular Complications of Type 2 Diabetes Mellitus
Authors: Bielska Agnieszka , Niemira Magdalena , Kretowski Adam
Published: March 2021

Type 2 diabetes mellitus (T2DM) and its complications pose a serious threat to the life and health of patients around the world. The most dangerous complications of this disease are vascular complications. Microvascular complications of T2DM include retinopathy, nephropathy, and neuropathy. In turn, macrovascular complications include coronary artery disease, peripheral artery disease, and cerebrovascular disease. The currently used diagnostic methods do not ensure detection of the disease at an early stage, and they also do not predict the risk of developing specific complications. MicroRNAs (miRNAs) are small, endogenous, noncoding molecules that are involved in key processes, such as cell proliferation, differentiation, and apoptosis. Recent research has assigned them an important role as potential biomarkers for detecting complications related to diabetes. We suggest that utilizing miRNAs can be a routine approach for early diagnosis and prognosis of diseases and may enable the development of better therapeutic approaches. In this paper, we conduct a review of the latest reports demonstrating the usefulness of miRNAs as biomarkers in the vascular complications of T2DM.
Journal of Cybersecurity and Privacy: A New Open Access Journal
Authors: Rawat Danda B.
Published: March 2021

The Cybersecurity and Privacy field has been one of the most critical parts of our lives and modern society at large [...]
On the Omega Distribution: Some Properties and Estimation
Authors: Alsubie Abdelaziz , Akhter Zuber , Athar Haseeb , Alam Mahfooz , Ahmad Abd EL-Baset A. , Cordeiro Gauss M. , Afify Ahmed Z.
Published: March 2021

We obtain explicit expressions for single and product moments of the order statistics of an omega distribution. We also discuss seven methods to estimate the omega parameters. Various simulation results are performed to compare the performance of the proposed estimators. Furthermore, the maximum likelihood method is adopted to estimate the omega parameters under the type II censoring scheme. The usefulness of the omega distribution is proven using a real data set.
Models for COVID-19 Daily Confirmed Cases in Different Countries
Authors: Ahmed Hamdy M. , Elbarkouky Reda A. , Omar Othman A. M. , Ragusa Maria Alessandra
Published: March 2021

In this paper, daily confirmed cases of COVID-19 in different countries are modelled using different mathematical regression models. The curve fitting is used as a prediction tool for modeling both past and upcoming coronavirus waves. According to virus spreading and average annual temperatures, countries under study are classified into three main categories. First category, the first wave of the coronavirus takes about two-year seasons (about 180 days) to complete a viral cycle. Second category, the first wave of the coronavirus takes about one-year season (about 90 days) to complete the first viral cycle with higher virus spreading rate. These countries take stopping periods with low virus spreading rate. Third category, countries that take the highest virus spreading rate and the viral cycle complete without stopping periods. Finally, predictions of different upcoming scenarios are made and compared with actual current smoothed daily confirmed cases in these countries.
A Review of Tracking and Trajectory Prediction Methods for Autonomous Driving
Authors: Leon Florin , Gavrilescu Marius
Published: March 2021

This paper provides a literature review of some of the most important concepts, techniques, and methodologies used within autonomous car systems. Specifically, we focus on two aspects extensively explored in the related literature: tracking, i.e., identifying pedestrians, cars or obstacles from images, observations or sensor data, and prediction, i.e., anticipating the future trajectories and motion of other vehicles in order to facilitate navigating through various traffic conditions. Approaches based on deep neural networks and others, especially stochastic techniques, are reported.
Phagocytosis of Astaxanthin-Loaded Microparticles Modulates TGFβ Production and Intracellular ROS Levels in J774A.1 Macrophages
Authors: Binatti Eleonora , Zoccatelli Gianni , Zanoni Francesca , Donà Giulia , Mainente Federica , Chignola Roberto
Published: March 2021

Radiation-induced fibrosis is a serious long-lasting side effect of radiation therapy. Central to this condition is the role of macrophages that, activated by radiation-induced reactive oxygen species and tissue cell damage, produce pro-inflammatory cytokines, such as transforming growth factor beta (TGFβ). This, in turn, recruits fibroblasts at the site of the lesion that initiates fibrosis. We investigated whether astaxanthin, an antioxidant molecule extracted from marine and freshwater organisms, could help control macrophage activation. To this purpose, we encapsulated food-grade astaxanthin from Haematococcus pluvialis into micrometer-sized whey protein particles to specifically target macrophages that can uptake material within this size range by phagocytosis. The data show that astaxanthin-loaded microparticles are resistant to radiation, are well-tolerated by J774A.1 macrophages, induce in these cells a significant reduction of intracellular reactive oxygen species and inhibit the release of active TGFβ as evaluated in a bioassay with transformed MFB-F11 fibroblasts. Micro-encapsulation of bioactive molecules is a promising strategy to specifically target phagocytic cells and modulate their own functions.
The Missing Protein: Is T-Cadherin a Previously Unknown GPI-Anchored Receptor on Platelets?
Authors: Balatskaya Maria N. , Baglay Alexandra I. , Balatskiy Alexander V.
Published: March 2021

The membrane of platelets contains at least one uncharacterized glycosylphosphatidylinositol (GPI)-anchored protein according to the literature. Moreover, there is not enough knowledge on the receptor of low-density lipoproteins (LDL) mediating rapid Ca2+ signaling in platelets. Coincidentally, expression of a GPI-anchored protein T-cadherin increases LDL-induced Ca2+ signaling in nucleated cells. Here we showed evidence that supports the hypothesis about the presence of T-cadherin on platelets. The presence of T-cadherin on the surface of platelets and megakaryocytes was proven using antibodies whose specificity was tested on several negative and positive control cells by flow cytometry and confocal microscopy. Using phosphatidylinositol-specific phospholipase C, the presence of glycosylphosphatidylinositol anchor in the platelet T-cadherin form as well as in other known forms was confirmed. We showed by immunoblotting that the significant part of T-cadherin was detected in specific membrane domains (detergent Triton X-114 resistant) and the molecular weight of this newly identified protein was greater than that of T-cadherin from nucleated cells. Nevertheless, polymerase chain reaction data confirmed only the presence of isoform-1 of T-cadherin in platelets and megakaryocytes, which was also present in nucleated cells. We observed the redistribution of this newly identified protein after the activation of platelets, but only further work may explain its functional importance. Thus, our data described T-cadherin with some post-translational modifications as a new GPI-anchored protein on human platelets.
The Effects of Chemical Etching and Ultra-Fine Grain Structure of Titanium on MG-63 Cells Response
Authors: Nazarov Denis , Zemtsova Elena , Smirnov Vladimir , Mitrofanov Ilya , Maximov Maxim , Yudintceva Natalia , Shevtsov Maxim
Published: March 2021

In this work, we study the influence of the surface properties of ultrafine grained (UFG) and coarse grained (CG) titanium on the morphology, viability, proliferation and differentiation of osteoblast-like MG-63 cells. Wet chemical etching in H2SO4/H2O2 and NH4OH/H2O2 solutions was used for producing surfaces with varying morphology, topography, composition and wettability. The topography and morphology have been studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The composition was determined by time of flight mass-spectrometry (TOF-SIMS) and X-ray photoelectron spectroscopy (XPS). The results showed that it is possible to obtain samples with different compositions, hydrophilicity, topography and nanoscale or/and microscale structures by changing the etching time and the type of etching solution. It was found that developed topography and morphology can improve spreading and proliferation rate of MG-63 cells. A significant advantage of the samples of the UFG series in comparison with CG in adhesion, proliferation at later stages of cultivation (7 days), higher alkaline phosphatase (ALP) activity and faster achievement of its maximum values was found. However, there is no clear benefit of the UFG series on osteopontin (OPN) expression. All studied samples showed no cytotoxicity towards MG-63 cells and promoted their osteogenic differentiation.
Analysis of Threshold Voltage Shift for Full VGS/VDS/Oxygen-Content Span under Positive Bias Stress in Bottom-Gate Amorphous InGaZnO Thin-Film Transistors
Authors: Kim Je-Hyuk , Jang Jun Tae , Bae Jong-Ho , Choi Sung-Jin , Kim Dong Myong , Kim Changwook , Kim Yoon , Kim Dae Hwan
Published: March 2021

In this study, we analyzed the threshold voltage shift characteristics of bottom-gate amorphous indium-gallium-zinc-oxide (IGZO) thin-film transistors (TFTs) under a wide range of positive stress voltages. We investigated four mechanisms: electron trapping at the gate insulator layer by a vertical electric field, electron trapping at the drain-side GI layer by hot-carrier injection, hole trapping at the source-side etch-stop layer by impact ionization, and donor-like state creation in the drain-side IGZO layer by a lateral electric field. To accurately analyze each mechanism, the local threshold voltages of the source and drain sides were measured by forward and reverse read-out. By using contour maps of the threshold voltage shift, we investigated which mechanism was dominant in various gate and drain stress voltage pairs. In addition, we investigated the effect of the oxygen content of the IGZO layer on the positive stress-induced threshold voltage shift. For oxygen-rich devices and oxygen-poor devices, the threshold voltage shift as well as the change in the density of states were analyzed.
Potential of Tailing Deposits in Chile for the Sequestration of Carbon Dioxide Produced by Power Plants Using Ex-Situ Mineral Carbonation
Authors: Marín Oscar , Valderrama José O. , Kraslawski Andrzej , Cisternas Luis A.
Published: March 2021

In this study, the potential of copper tailing deposits in Chile for the sequestration of carbon dioxide (CO2) via ex-situ mineral carbonation integrating the recovery of valuable metals was assessed. An inventory of tailing deposits and CO2 sources existing in Chile was constructed to determine the most suitable site for the installation of a future mineral carbonation plant and to evaluate the technical, economic, and environmental feasibility of CO2 capture, separation, and transport from the source to the mineral carbonation plant. The data of the inventory of tailings deposits in Chile were obtained from the National Service of Geology and Mining. For the thermoelectric plants installed in Chile, data of energy production were obtained from the Energy National Commission. Through the use of the technique for order preference by similarity to ideal solution (TOPSIS) method and sensitivity analysis, the optimum location in the region of Antofagasta to install a mineral carbonation plant was identified. In addition, the results show that in the region of Antofagasta five tailing deposits have the potential to sequester between 66 to 99 Mt of CO2. Meanwhile, thermoelectric plants in 2018 produced about 9.4 Mt of CO2 that is available to be sequestered, with a maximum generation potential of 21.9 Mt of CO2eq per year. The methodology and the study presented can be considered as a preliminary study to identify tailings that require further analysis.
Unveiling Carbon Dioxide and Ethanol Diffusion in Carbonated Water-Ethanol Mixtures by Molecular Dynamics Simulations
Authors: Ahmed Khaireh Mohamed , Angot Marie , Cilindre Clara , Liger-Belair Gérard , Bonhommeau David A.
Published: March 2021

The diffusion of carbon dioxide (CO2) and ethanol (EtOH) is a fundamental transport process behind the formation and growth of CO2 bubbles in sparkling beverages and the release of organoleptic compounds at the liquid free surface. In the present study, CO2 and EtOH diffusion coefficients are computed from molecular dynamics (MD) simulations and compared with experimental values derived from the Stokes-Einstein (SE) relation on the basis of viscometry experiments and hydrodynamic radii deduced from former nuclear magnetic resonance (NMR) measurements. These diffusion coefficients steadily increase with temperature and decrease as the concentration of ethanol rises. The agreement between theory and experiment is suitable for CO2. Theoretical EtOH diffusion coefficients tend to overestimate slightly experimental values, although the agreement can be improved by changing the hydrodynamic radius used to evaluate experimental diffusion coefficients. This apparent disagreement should not rely on limitations of the MD simulations nor on the approximations made to evaluate theoretical diffusion coefficients. Improvement of the molecular models, as well as additional NMR measurements on sparkling beverages at several temperatures and ethanol concentrations, would help solve this issue.
Development of New Targeted Inulin Complex Nanoaggregates for siRNA Delivery in Antitumor Therapy
Authors: Cavallaro Gennara , Sardo Carla , Craparo Emanuela Fabiola , Giammona Gaetano
Published: March 2021

Here, a novel strategy of formulating efficient polymeric carriers based on the already described INU-IMI-DETA for gene material whose structural, functional, and biological properties can be modulated and improved was successfully investigated. In particular, two novel derivatives of INU-IMI-DETA graft copolymer were synthesized by chemical functionalisation with epidermal growth factor (EGF) or polyethylenglycol (PEG), named INU-IMI-DETA-EGF and INU-IMI-DETA-PEG, respectively, in order to improve the performance of already described “inulin complex nanoaggregates” (ICONs). The latter were thus prepared by appropriately mixing the two copolymers, by varying each component from 0 to 100 wt% on the total mixture, named EP-ICONs. It was seen that the ability of the INU-IMI-DETA-EGF/INU-IMI-DETA-PEG polymeric mixture to complex siGL3 increases with the increase in the EGF-based component in the EP-ICONs and, for each sample, with the increase in the copolymer:siRNA weight ratio (R). On the other hand, the susceptibility of loaded siRNA towards RNase decreases with the increase in the pegylated component in the polymeric mixture. At all R values, the average size and the zeta potential values are suitable for escaping from the RES system and suitable for prolonged intravenous circulation. By means of biological characterisation, it was shown that MCF-7 cells are able to internalize mainly the siRNA-loaded into EGF-decorated complexes, with a significant difference from ICONs, confirming its targeting function. The targeting effect of EGF on EP-ICONs was further demonstrated by a competitive cell uptake study, i.e., after cell pre-treatment with EGF. Finally, it was shown that the complexes containing both EGF and PEG are capable of promoting the internalisation and therefore the transfection of siSUR, a siRNA acting against surviving mRNA, and to increase the sensitivity to an anticancer agent, such as doxorubicin.
Lupin Protein Isolate Structure Diversity in Frozen-Cast Foams: Effects of Transglutaminases and Edible Fats
Authors: Ceresino Elaine Berger , Johansson Eva , Sato Hélia Harumi , Plivelic Tomás S. , Hall Stephen A. , Bez Jürgen , Kuktaite Ramune
Published: March 2021

This study addresses an innovative approach to generate aerated foods with appealing texture through the utilization of lupin protein isolate (LPI) in combination with edible fats. We show the impact of transglutaminases (TGs; SB6 and commercial), glycerol (Gly), soy lecithin (Lec) and linoleic acid (LA) on the micro- and nanostructure of health promoting solid foods created from LPI and fats blends. 3-D tomographic images of LPI with TG revealed that SB6 contributed to an exceptional bubble spatial organization. The inclusion of Gly and Lec decreased protein polymerization and also induced the formation of a porous layered material. LA promoted protein polymerization and formation of homogeneous thick layers in the LPI matrix. Thus, the LPI is a promising protein resource which when in blend with additives is able to create diverse food structures. Much focus has been placed on the great foamability of LPI and here we show the resulting microstructure of LPI foams, and how these were improved with addition of TGs. New food applications for LPI can arise with the addition of food grade dispersant Lec and essential fatty-acid LA, by improved puffiness, and their contributing as replacer of chemical leavening additives in gluten-free products.
Exploring Charged Polymeric Cyclodextrins for Biomedical Applications
Authors: Bognanni Noemi , Bellia Francesco , Viale Maurizio , Bertola Nadia , Vecchio Graziella
Published: March 2021

Over the years, cyclodextrin uses have been widely reviewed and their proprieties provide a very attractive approach in different biomedical applications. Cyclodextrins, due to their characteristics, are used to transport drugs and have also been studied as molecular chaperones with potential application in protein misfolding diseases. In this study, we designed cyclodextrin polymers containing different contents of β- or γ-cyclodextrin, and a different number of guanidinium positive charges. This allowed exploration of the influence of the charge in delivering a drug and the effect in the protein anti-aggregant ability. The polymers inhibit Amiloid β peptide aggregation; such an ability is modulated by both the type of CyD cavity and the number of charges. We also explored the effect of the new polymers as drug carriers. We tested the Doxorubicin toxicity in different cell lines, A2780, A549, MDA-MB-231 in the presence of the polymers. Data show that the polymers based on γ-cyclodextrin modified the cytotoxicity of doxorubicin in the A2780 cell line.
Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) Acinetobacter baumannii
Authors: Yap Phui-Chyng , Ayuhan Noorfazlin , Woon Jia Jie , Teh Cindy Shuan Ju , Lee Vannajan Sanghiran , Azman Adzzie Shazleen , AbuBakar Sazaly , Lee Hai Yen
Published: March 2021

A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
Health Potential of Clery Strawberries: Enzymatic Inhibition and Anti-Candida Activity Evaluation
Authors: Cairone Francesco , Simonetti Giovanna , Orekhova Anastasia , Casadei Maria Antonietta , Zengin Gokhan , Cesa Stefania
Published: March 2021

Strawberries, belonging to cultivar Clery (Fragaria × ananassa Duchesne ex Weston) and to a graft obtained by crossing Clery and Fragaria vesca L., were chosen for a study on their health potential, with regard to the prevention of chronic and degenerative diseases. Selected samples, coming from fresh and defrosted berries, submitted to different homogenization techniques combined with thermal and microwave treatments, had been previously analyzed in their polyphenolic content and antioxidant capacity. In the present work, these homogenates were evaluated in relation to their enzymatic inhibition activity towards acetylcholinesterase and butyrylcholinesterase, α-amylase, α-glucosidase and tyrosinase. All these enzymes, involved in the onset of diabetes, and neurodegenerative and other chronic diseases, were modulated by the tested samples. The inhibitory effect on tyrosinase and cholinesterase was the most valuable. Antifungal activity against Candida albicans, recently shown to play a crucial role in human gut diseases as well as diabetes, rheumatoid arthritis and Alzheimer’s disease, was also shown in vitro and confirmed by the in vivo text on Galleria mellonella. Overall, the obtained results confirm once again the health potential of strawberries; however, the efficacy is dependent on high quality products submitted to correct processing flow charts.
QSAR Models for Active Substances against Pseudomonas aeruginosa Using Disk-Diffusion Test Data
Authors: Bugeac Cosmin Alexandru , Ancuceanu Robert , Dinu Mihaela
Published: March 2021

Pseudomonas aeruginosa is a Gram-negative bacillus included among the six “ESKAPE” microbial species with an outstanding ability to “escape” currently used antibiotics and developing new antibiotics against it is of the highest priority. Whereas minimum inhibitory concentration (MIC) values against Pseudomonas aeruginosa have been used previously for QSAR model development, disk diffusion results (inhibition zones) have not been apparently used for this purpose in the literature and we decided to explore their use in this sense. We developed multiple QSAR methods using several machine learning algorithms (support vector classifier, K nearest neighbors, random forest classifier, decision tree classifier, AdaBoost classifier, logistic regression and naïve Bayes classifier). We used four sets of molecular descriptors and fingerprints and three different methods of data balancing, together with the “native” data set. In total, 32 models were built for each set of descriptors or fingerprint and balancing method, of which 28 were selected and stacked to create meta-models. In terms of balanced accuracy, the best performance was provided by KNN, logistic regression and decision tree classifier, but the ensemble method had slightly superior results in nested cross-validation.
Chemical Adsorption Strategy for DMC-MeOH Mixture Separation
Authors: Zhang Fucan , Liu Ping , Zhang Kan , Song Qing-Wen
Published: March 2021

The effective separation of dimethyl carbonate (DMC) from its methanol mixture through simple, inexpensive and low energy-input method is a promising and challenging field in the process of organic synthesis. Herein, a reversible adsorption strategy through the assistance of superbase and CO2 for DMC/methanol separation at ambient condition was described. The process was demonstrated effectively via the excellent CO2 adsorption efficiency. Notably, the protocol was also suitable to other alcohol (i.e., monohydric alcohol, dihydric alcohol, trihydric alcohol) mixtures. The study provided guidance for potential separation of DMC/alcohol mixture in the scale-up production.
A Multifactorial Approach to Untangle Graphene Oxide (GO) Nanosheets Effects on Plants: Plant Growth-Promoting Bacteria Inoculation, Bacterial Survival, and Drought
Authors: Lopes Tiago , Cruz Catarina , Cardoso Paulo , Pinto Ricardo , Marques Paula A. A. P. , Figueira Etelvina
Published: March 2021

Drought is a limiting factor for agricultural productivity. Climate change threatens to expand the areas of the globe subjected to drought, as well as to increase the severity and duration of water shortage. Plant growth-promoting bacteria (PGPB) are widely studied and applied as biostimulants to increase plant production and to enhance tolerance to abiotic and biotic constraints. Besides PGPB, studies on the potential of nanoparticles to be used as biostimulants are also thriving. However, many studies report toxicity of tested nanoparticles in bacteria and plants in laboratory conditions, but few studies have reported effects of nanoparticles towards bacterial cells and communities in the soil. The combined application of nanoparticles and PGPB as biostimulant formulations are poorly explored and it is important to unravel the potentialities of their combined application as a way to potentiate food production. In this study, Rhizobium sp. E20-8 and graphene oxide (GO) nanosheets were applied on container-grown maize seedlings in watered and drought conditions. Bacterial survival, seedling growth (dry weight), and biochemical endpoints (photosynthetic pigments, soluble and insoluble carbohydrates, proline, lipid peroxidation, protein, electron transport system, and superoxide dismutase) were evaluated. Results showed that the simultaneous exposure to GO and Rhizobium sp. E20-8 was able to alleviate the stress induced by drought on maize seedlings through osmotic and antioxidant protection by GO and mitigation of GO effects on the plant’s biochemistry by Rhizobium sp. E20-8. These results constitute a new lead on the development of biostimulant formulations to improve plant performance and increase food production in water-limited conditions.
The Non-Coding RNA Journal Club: Highlights on Recent Papers-8
Authors: Tarallo Sonia , Pardini Barbara , Fox Archa H. , Ingram Hayley , Taccioli Cristian , Taube Joseph H. , Mani Sendurai A.
Published: March 2021

We are glad to share with you our eighth Journal Club and to highlight some of the most interesting papers published recently [...]
The Role of the Catalyst on the Reactivity and Mechanism in the Diels–Alder Cycloaddition Step of the Povarov Reaction for the Synthesis of a Biological Active Quinoline Derivative: Experimental and Theoretical Investigations
Authors: Lamri Soumia , Heddam Affaf , Kara Meriem , Yahia Wassila , Khorief Nacereddine Abdelmalek
Published: March 2021

An experimental and theoretical study of the reactivity and mechanism of the non-catalyzed and catalyzed Povarov reaction for the preparation of a 4-ethoxy-2,3,4,4a-tetrahydro-2-phenylquinoline as a biological active quinoline derivative has been performed. The optimization of experimental conditions indicate that the use of a catalyst, namely Lewis acid with an electron-releasing group, creates the best experimental conditions for this kind of reaction. The chemical structure was characterized by the usual spectroscopic methods. The prepared quinoline derivative has been also tested in vitro for antibacterial activity, which displays moderate inhibitory activity against both Escherichia coli and Staphylococcus aureus. The antioxidant activity was investigated in vitro by evaluating their reaction with 1,1-diphenyl-2-picrylhydrazyl DPPH radical, which reveals high reactivity. The computational study was performed on the Diels–Alder step of the Povarov reaction using a B3LYP/6-31G(d,p) level of theory. The conceptual DFT reactivity indices explain well the reactivity and the meta regioselectivity experimentally observed. Both catalysts enhance the reactivity of the imine, favoring the formation of the meta regioisomers with a low activation energy, and they change the mechanism to highly synchronous for the Lewis acid and to stepwise for the Brønsted acid. The reaction of imine with allyl alcohol does not give any product, which requires high activation energy.
Re-Introduction of Bovine Viral Diarrhea Virus in a Disease-Free Region: Impact on the Affected Cattle Herd and Diagnostic Implications
Authors: Albrecht Kerstin , Linder Miriam , Heinrich Anja , Höche Jennifer , Beer Martin , Gaede Wolfgang , Wernike Kerstin
Published: March 2021

Bovine viral diarrhea (BVD) is one of the most important infectious cattle diseases worldwide. The major source of virus transmission is immunotolerant, persistently infected (PI) calves, which makes them the key target of control programs. In the German federal state of Saxony-Anhalt, a very low prevalence was achieved, with more than 99.8% of the cattle herds being free from PI animals since the year 2013. In 2017, BVD virus was detected in a previously disease-free holding (herd size of ~380 cows, their offspring, and fattening bulls). The purchase of two so-called Trojan cows, i.e., dams pregnant with a PI calf, was identified as the source of infection. The births of the PI animals resulted in transient infections of in-contact dams, accompanied by vertical virus transmission to their fetuses within the critical timeframe for the induction of PI calves. Forty-eight days after the birth of the first PI calf, all animals in close contact with the Trojan cows during their parturition period were blood-sampled and serologically examined by a neutralization test and several commercial ELISAs. The resulting seroprevalence strongly depended on the applied test system. The outbreak could be stopped by the immediate elimination of every newborn PI calf and vaccination, and since 2018, no BVD cases have occurred.
Genomic Analyses of Globodera pallida, A Quarantine Agricultural Pathogen in Idaho
Authors: Wasala Sulochana K. , Howe Dana K. , Dandurand Louise-Marie , Zasada Inga A. , Denver Dee R.
Published: March 2021

Globodera pallida is among the most significant plant-parasitic nematodes worldwide, causing major damage to potato production. Since it was discovered in Idaho in 2006, eradication efforts have aimed to contain and eradicate G. pallida through phytosanitary action and soil fumigation. In this study, we investigated genome-wide patterns of G. pallida genetic variation across Idaho fields to evaluate whether the infestation resulted from a single or multiple introduction(s) and to investigate potential evolutionary responses since the time of infestation. A total of 53 G. pallida samples (~1,042,000 individuals) were collected and analyzed, representing five different fields in Idaho, a greenhouse population, and a field in Scotland that was used for external comparison. According to genome-wide allele frequency and fixation index (Fst) analyses, most of the genetic variation was shared among the G. pallida populations in Idaho fields pre-fumigation, indicating that the infestation likely resulted from a single introduction. Temporal patterns of genome-wide polymorphisms involving (1) pre-fumigation field samples collected in 2007 and 2014 and (2) pre- and post-fumigation samples revealed nucleotide variants (SNPs, single-nucleotide polymorphisms) with significantly differentiated allele frequencies indicating genetic differentiation. This study provides insights into the genetic origins and adaptive potential of G. pallida invading new environments.
Effects of Maternal Environment on Seed Germination and Seedling Vigor of Petunia × hybrida under Different Abiotic Stresses
Authors: Nguyen Chi D. , Chen Jianjun , Clark David , Perez Hector , Huo Heqiang (Alfred)
Published: March 2021

Seed germination and seedling vigor can be affected by environmental cues experienced by the mother plant. However, information about how the maternal environment affects seed quality is scarce in ornamental plants. This study aimed to investigate the effects of two different maternal environments on the seed germination and seedling vigor of Petunia × hybrida under a variety of abiotic stresses. Petunia mother plants were grown in either a greenhouse during the summer months or an indoor controlled-temperature-and-light environment. Collected seeds were subjected to external stressors, including polyethylene glycol (PEG), sodium chloride (NaCl), high temperature, and abscisic acid (ABA), to determine seed germination percentage and seedling vigor. Results indicated that seeds harvested from the mother plants grown in a controlled environment germinated better than seeds harvested from the mother plants grown in the greenhouse when suboptimal germination conditions were applied. Additionally, the seedlings from the controlled maternal environment performed better in both ABA and salinity stress tests than the greenhouse seedlings. Interestingly, the greenhouse seedlings displayed less reactive oxygen species (ROS) damage and lower electrolyte leakage than the controlled environment seedlings under dehydration stress. The difference in germination and seedling vigor of seeds from the two different maternal environments might be due to the epigenetic memory inherited from the mother plants. This study highlighted the strong impact of the maternal environment on seed germination and seedling vigor in Petunia and may assist in high-quality seed production in ornamental plants.
Contribution of Essential Oils to the Fight against Microbial Biofilms—A Review
Authors: Nuță Diana Camelia , Limban Carmen , Chiriță Cornel , Chifiriuc Mariana Carmen , Costea Teodora , Ioniță Petre , Nicolau Ioana , Zarafu Irina
Published: March 2021

The increasing clinical use of artificial medical devices raises the issue of microbial contamination, which is a risk factor for the occurrence of biofilm-associated infections. A huge amount of scientific data highlights the promising potential of essential oils (EOs) to be used for the development of novel antibiofilm strategies. We aimed to review the relevant literature indexed in PubMed and Embase and to identify the recent directions in the field of EOs, as a new modality to eradicate microbial biofilms. We paid special attention to studies that explain the mechanisms of the microbicidal and antibiofilm activity of EOs, as well as their synergism with other antimicrobials. The EOs are difficult to test for their antimicrobial activity due to lipophilicity and volatility, so we have presented recent methods that facilitate these tests. There are presented the applications of EOs in chronic wounds and biofilm-mediated infection treatment, in the food industry and as air disinfectants. This analysis concludes that EOs are a source of antimicrobial agents that should not be neglected and that will probably provide new anti-infective therapeutic agents.
Variable Selection for Fault Detection Based on Causal Discovery Methods: Analysis of an Actual Industrial Case
Authors: Clavijo Nayher , Melo Afrânio , Soares Rafael M. , Campos Luiz Felipe de O. , Lemos Tiago , Câmara Maurício M. , Anzai Thiago K. , Diehl Fabio C. , Thompson Pedro H. , Pinto José Carlos
Published: March 2021

Variable selection constitutes an essential step to reduce dimensionality and improve performance of fault detection and diagnosis in large scale industrial processes. For this reason, in this paper, variable selection approaches based on causality are proposed and compared, in terms of model adjustment of available data and fault detection performance, with several other filter-based, wrapper-based, and embedded-based variable selection methods. These approaches are applied in a simulated benchmark case and an actual oil and gas industrial case considering four different learning models. The experimental results show that obtained models presented better performance during the fault detection stage when variable selection procedures based on causality were used for purpose of model building.
Agronomic Characteristics of the Compost-Bedded Pack Made with Forest Biomass or Sawdust
Authors: Llonch Lourdes , Gordo Cecilia , López Marga , Castillejos Lorena , Ferret Alfred , Balanyà Teresa
Published: March 2021

To ascertain the agronomic value of the material resulting from the compost-bedded pack (CBP) in dairy barns, a cross-over experiment was designed with eight dry non-pregnant Holstein cows. The study was performed in two 11-week periods. Bedding materials used were: (1) CBP with sawdust (S) and (2) CBP with forest biomass (FB). Samples were taken from the raw bedding materials and from the CBP across the experiment. We conducted an additional study preparing two piles, one of each CBP material, to accomplish a composting process of 3 months, where samples were also taken. Granulometry and some chemical composition characteristics of FB made it a suitable bedding material to be used as CBP, but its high moisture content limited the ability to absorb liquid manure. Both the degree of stability of the organic matter and the temperature evolution of CBP suggest that a real composting process did not occur. Finally, the composting process of the piles did not lead to any relevant change in CBP materials. From the agronomic point of view, S and FB present potentially valuable characteristics as regards organic amendment in the soil, thanks to their high organic matter content and low nutrient content.
Black Holes as Evidence of God’s Care
Authors: Ross Hugh
Published: March 2021

As black holes gravitationally draw matter toward their event horizons, a high proportion of this matter is converted into energy. Radiation from this conversion process is deadly for advanced life. The apparent incompatibility of black holes with advanced life raises a problem for Christians and other theists who believe that God planned the rise of advanced life on Earth. Yet additional scientific data may help to resolve this apparent problem. This article argues that a universe with the mass and laws and constants of physics to make advanced life possible will inevitably produce black holes, and this is good news. When the most massive stars and merging neutron stars become black holes, they manufacture elements heavier than iron. Eight of these r-process elements appear essential for advanced life; the remainder appear essential for enduring life and for advanced civilization. Moreover, though black holes produce deadly radiation in all known regions of the universe where advanced life is conceivable, our solar system is protected from this deadly radiation. By apparent fine-tuning, we live in a uniquely safe and uniquely provisioned location. These scientific findings suggest a way that theists can reconcile the existence of black holes with the existence of a Creator.
Ritual Lamentation in the Irish Penitentials
Authors: Bergholm Alexandra
Published: March 2021

Some of the earliest references to ritual lamentation or keening in the early Irish sources are found in the penitential handbooks dated to around the seventh and eighth centuries. In previous scholarship, these passages have commonly been interpreted as evidence of the continuous attempts of the Church to curb pagan practices among the ‘nominally Christian’ populace, thus assuming that such regulations were primarily used as a means of social control. This article examines the wider theological and intellectual context of these texts, by focusing in particular on the influence of the Old Testament on early Irish ecclesiastical writing. It will be argued that the demonstrable preoccupation of these sources with issues such as ritual purity and proper religious observance suggests that the stipulations pertaining to lamentation were not solely intended to regulate lay behavior.