bluuebunny/crossref_metadata_2025 huggingface dataset:
Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France
Authors: Sarda-Estève Roland , Baisnée Dominique , Guinot Benjamin , Mainelis Gediminas , Sodeau John , O’Connor David , Besancenot Jean Pierre , Thibaudon Michel , Monteiro Sara , Petit Jean-Eudes , Gros Valérie
Published: August 2020

Background: The monitoring of bioaerosol concentrations in the air is a relevant endeavor due to potential health risks associated with exposure to such particles and in the understanding of their role in climate. In this context, the atmospheric concentrations of bacteria were measured from January 2018 to May 2020 at Saclay, France. The aim of the study was to understand the seasonality, the daily variability, and to identify the geographical origin of airborne bacteria. Methods: 880 samples were collected daily on polycarbonate filters, extracted with purified water, and analyzed using the cultivable method and flow cytometry. A source receptor model was used to identify the origin of bacteria. Results: A tri-modal seasonality was identified with the highest concentrations early in spring and over the summer season with the lowest during the winter season. Extreme changes occurred daily due to rapid changes in meteorological conditions and shifts from clean air masses to polluted ones. Conclusion: Our work points toward bacterial concentrations originating from specific seasonal-geographical ecosystems. During pollution events, bacteria appear to rise from dense urban areas or are transported long distances from their sources. This key finding should drive future actions to better control the dispersion of potential pathogens in the air, like persistent microorganisms originating from contaminated areas.
Assessment of Alcohol Consumption and Anxiety as Predictors of Risk of Anorexia and Bulimia in Non-Clinicals Samples
Authors: Pineda-García Gisela , Ochoa-Ruiz Estefanía , Gómez-Peresmitré Gilda , Platas-Acevedo Silvia
Published: August 2020

The objective of this study is to assess the effect of alcohol consumption, anxiety, and food restriction before and after consuming alcohol and body image on the risk of anorexia and bulimia in college students from Tijuana, Baja California, through predictive statistical models. A quantitative, descriptive, and cross-sectional design and a non-probabilistic sample of 526 college students from Tijuana, Baja California, México were used. Application of the scales (with acceptable psychometric properties) was conducted in classrooms. Through path analyses, four models were found with adequate indicators of goodness of fit: (1) risk of anorexia in women [Chi Square (X2) = 5.34, p = 0.376, Adjusted Determination Coefficient (R2)= 0.250]; (2) anorexia risk for men (X2 = 13.067, p = 0.192, R2 = 0.058); (3) risk of bulimia in women (X2 = 3.358, p = 0.645, R2 = 0.202); and bulimia risk for men (X2 = 14.256, p = 0.075, R2 = 0.284). The findings provide empirical evidence for the food and alcohol disturbance model.
Goalkeepers Live Longer than Field Players: A Retrospective Cohort Analysis Based on World-Class Football Players
Authors: Śmigielski Witold , Gajda Robert , Małek Łukasz , Drygas Wojciech
Published: August 2020

The purpose of this article is to study whether the position occupied by footballers on the pitch influences their life duration. It is known that various types of sporting activity (endurance, resistance, or mixed) may influence lifespan in different ways. However, there is a paucity of data regarding the role of different positions played in team sports such as football. Our research was based on elite international football players born before 1923 who took part in the first three football World Cups (n = 443) or played in the 1946/1947 season in the leading clubs of the main European leagues (n = 280). Goalkeepers were characterized by a 5–8-year longer life duration compared to their colleagues playing in other positions (World Cup: 82.0 ± 7.0 vs. 74.0 ± 8.0, p = 0.0047; European leagues: 83.0 ± 7.5 vs. 78.0 ± 8.0, p = 0.0023), with an absence of differences between defenders, midfielders, and forwards. Moreover, in both of the analyzed subgroups, the rate of survival until the 85th birthday was significantly higher among goalkeepers than among field players (p = 0.0102 and p = 0.0048, for both studied groups, respectively).
Acceptability and Feasibility of Best Practice School Lunches by Elementary School-Aged Children in a Serve Setting: A Randomized Crossover Trial
Authors: Joyce Jillian M. , Harris Kyleen , Mailey Emily L. , Rosenkranz Richard R. , Rosenkranz Sara K.
Published: August 2020

Background: National School Lunch Program (NSLP) standards have improved school lunch dietary quality (DQ), however, further improvements could be made. Acceptability and feasibility of higher DQ are potential barriers. Thus, the purpose is to compare acceptability and feasibility of best practice (BPSL, optimizing DQ) with typical school lunches (TSL, meeting minimum NSLP standards) served separately and concurrently. Methods: Forty elementary school-aged participants were recruited for a randomized crossover trial. Participants attended three meal conditions (MC) choosing one of two meal types—MC1) BPSL1/BPSL2, MC2) TSL1/TSL2, MC3) BPSL/TSL. Acceptability included taste test surveys, weighted plate waste assessments, and hunger scales. Feasibility included meal cost, time, and skill and equipment requirements. Results: There were no significant differences in total taste test score, average total plate waste, or change in hunger (ps > 0.017) before or after adjusting for covariates. TSL was selected significantly more often in MC3 (TSL = 83.3%, BPSL = 16.7%, p = 0.001). Meal cost (p = 0.783) and skill and equipment requirements were not significantly different between meal types. BPSL required significantly longer preparation time (TSL = 60 ± 25 min, BPSL = 267 ± 101 min, p = 0.026). Conclusions: Results indicate few differences in acceptability and feasibility between BPSL and TSL. This study could inform decision and policy-makers seeking to improve school lunch DQ and acceptance of higher DQ meals.
Handling of Fresh Vegetables: Knowledge, Hygienic Behavior of Vendors, Public Health in Maputo Markets, Mozambique
Authors: Salamandane Cátia , Fonseca Filipa , Afonso Sónia , Lobo Maria Luisa , Antunes Francisco , Matos Olga
Published: August 2020

In developing countries, markets are the main supply of horticultural products to populations, but this can pose a public health challenge due to the risk of the fecal-oral transmission of gut pathogens. This transmission is strongly associated with inadequate public sanitation or low standards of personal and domestic hygiene, and their prevalence can cause gastrointestinal diseases, which are the third leading cause of death in Mozambique. This study aims at assessing the risk for public health of horticultural products supply chain, from the farmers-vendors to the consumers, in municipal markets in Maputo-City, Mozambique. Surveys (75) were conducted on vendors and an observational analysis was performed in the markets under study. The results showed that 62% of the vendors had access to water from boreholes or artisanal sources and the issue “access to water” was significantly different between markets (p = 0.004). Of the vendors who wash their products (53.3%), only 7.5% use tap-water for this purpose, with the difference in attitudes being statistically significant between vendors in the markets (p = 0.035). The majority (60.4%) said that vegetables and fruits can cause diseases due to pesticides and only 31.3% believe that the diseases may be related to poor hygiene. Despite the vendors’ low knowledge of Good Hygiene Practices (GHP), we noticed that women have better practical assimilation of GHP when compared to men (p = 0.008). Although Maputo’s markets are struggling to achieve quality hygiene standards in a reliable and sustainable manner, their resources are limited and significantly different (p = 0.044) from market to market, and this problem remains a concern for the public-health authorities of the city. In conclusion, the provision of adequate drinking water and sewage disposal systems, together with education for health of vendors, can reduce the risk of contamination of fresh food by the more common organisms causing diarrhea in children, including intestinal parasites.
Parents’ Perceptions Regarding the Implementation of a Physical Therapy Stimulation Program for Children with Disabilities in Bolivia: A Qualitative Study
Authors: Pérez-de la Cruz Sagrario , Ramírez Ivonne
Published: September 2020

The purpose of this study was to explore how parents of children with neuromotor disorders in the department of Chuquisaca (Bolivia) perceive attendance to a physical therapy stimulation program and the expectations they place on the therapy and professional care provided to their children. Semi-structured interviews were conducted with the parents, related to their role in supporting the recommended exercise program for the child, generating topics such as benefits of the therapy for the child, impact on the family, and role of the project in terms of therapy and the physical therapists providing treatment, including both positive and negative aspects of the overall process. This study revealed the importance of understanding the feelings of families receiving intervention under a pioneering program in Bolivia for the detection and treatment of children with neuromotor disorders. Being able to access these types of services provides them with extensive personal, social, and economic support. Knowing their concerns, desires, and demands will allow us to continue to improve and offer the best care for children and families. The professionals involved should also be encouraged to develop effective teaching techniques to promote the inclusion of parents in the stimulation program.
Getting the Perpetrator Incorporated and Prioritized in Homicide Investigations: The Development and Evaluation of a Case-Specific Element Library (C-SEL)
Authors: Sutmuller August Daniel , den Hengst Marielle , Barros Ana Isabel , van Gelder Pieter
Published: September 2020

Homicide investigators in the digital era have access to an increasing amount of data and the processing of all persons of interest and pieces of evidence has become nearly impossible. This paper describes the development and evaluation of a case-specific element library (C-SEL) that can be used to incorporate and prioritize persons of interest in homicide investigations. In a survey, 107 experts in the field of criminal investigation assigned an initial score to the elements. Each element was extended with underlying factors that can be used to adjust the initial score based on the relevance and credibility of the source. A case study was conducted using three Dutch real-world cases to evaluate the methodology. The results look promising and are better than four methodologies currently used in practice.
Assessing Delivery of Selected Public Health Operations via Essential Public Health Operation Framework
Authors: Safi Mariam , Bertram Maja L. , Gulis Gabriel
Published: September 2020

Background: To assess the conduct of delivery of public health services at the municipal level in Denmark by applying services enlisted in the Essential Public Health Operation framework (EPHO) of WHO. Methods: We conducted individual qualitative interviews with key informants working with public health using a self-assessment survey tool in order to obtain an in-depth understanding of the interrelation or multidisciplinary work in Kolding Municipality. The developed self-assessment survey tool entailed questions about essential public health activities performed in a municipality. Results: The Municipality organizes and contributes to core service delivery EPHOs, namely health protection, health promotion, and disease prevention. It collaborates with the general practitioners and the Region of Southern Denmark, responsible for hospital care, to fulfill the selected EPHOs. Conclusions: To obtain a comprehensive picture of the organizations that deliver public health services within a municipality, it is necessary to conduct interviews with representatives from those organizations as well. Additionally, the results from this study can be used to improve the survey tool further and hereafter conduct a nationwide survey in Denmark, as well as other European countries.
Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics
Authors: Huang Haibo , Dai Cihai , Shen Hao , Gu Mingwei , Wang Yangjun , Liu Jizhu , Chen Liguo , Sun Lining
Published: August 2020

Since the cell was discovered by humans, it has been an important research subject for researchers. The mechanical response of cells to external stimuli and the biomechanical response inside cells are of great significance for maintaining the life activities of cells. These biomechanical behaviors have wide applications in the fields of disease research and micromanipulation. In order to study the mechanical behavior of single cells, various cell mechanics models have been proposed. In addition, the measurement technologies of single cells have been greatly developed. These models, combined with experimental techniques, can effectively explain the biomechanical behavior and reaction mechanism of cells. In this review, we first introduce the basic concept and biomechanical background of cells, then summarize the research progress of internal force models and experimental techniques in the field of cell mechanics and discuss the latest mechanical models and experimental methods. We summarize the application directions of cell mechanics and put forward the future perspectives of a cell mechanics model.
DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides
Authors: Si Can , Teixeira da Silva Jaime A. , He Chunmei , Yu Zhenming , Zhao Conghui , Wang Haobin , Zhang Mingze , Duan Jun
Published: August 2020

The acetylation or deacetylation of polysaccharides can influence their physical properties and biological activities. One main constituent of the edible medicinal orchid, Dendrobium officinale, is water-soluble polysaccharides (WSPs) with substituted O-acetyl groups. Both O-acetyl groups and WSPs show a similar trend in different organs, but the genes coding for enzymes that transfer acetyl groups to WSPs have not been identified. In this study, we report that REDUCED WALL ACETYLATION (RWA) proteins may act as acetyltransferases. Three DoRWA genes were identified, cloned, and sequenced. They were sensitive to abscisic acid (ABA), but there were no differences in germination rate and root length between wild type and 35S::DoRWA3 transgenic lines under ABA stress. Three DoRWA proteins were localized in the endoplasmic reticulum. DoRWA3 had relatively stronger transcript levels in organs where acetyl groups accumulated than DoRWA1 and DoRWA2, was co-expressed with polysaccharides synthetic genes, so it was considered as a candidate acetyltransferase gene. The level of acetylation of polysaccharides increased significantly in the seeds, leaves and stems of three 35S::DoRWA3 transgenic lines compared to wild type plants. These results indicate that DoRWA3 can transfer acetyl groups to polysaccharides and is a candidate protein to improve the biological activity of other edible and medicinal plants.
Ketones Elicit Distinct Alterations in Adipose Mitochondrial Bioenergetics
Authors: Walton Chase M. , Jacobsen Samuel M. , Dallon Blake W. , Saito Erin R. , Bennett Shantelle L. H. , Davidson Lance E. , Thomson David M. , Hyldahl Robert D. , Bikman Benjamin T.
Published: August 2020

Objective: The rampant growth of obesity worldwide has stimulated explosive research into human metabolism. Energy expenditure has been shown to be altered by diets differing in macronutrient composition, with low-carbohydrate, ketogenic diets eliciting a significant increase over other interventions. The central aim of this study was to explore the effects of the ketone β-hydroxybutyrate (βHB) on mitochondrial bioenergetics in adipose tissue. Methods: We employed three distinct systems—namely, cell, rodent, and human models. Following exposure to elevated βHB, we obtained adipose tissue to quantify mitochondrial function. Results: In every model, βHB robustly increased mitochondrial respiration, including an increase of roughly 91% in cultured adipocytes, 113% in rodent subcutaneous adipose tissue (SAT), and 128% in human SAT. However, this occurred without a commensurate increase in adipose ATP production. Furthermore, in cultured adipocytes and rodent adipose, we quantified and observed an increase in the gene expression involved in mitochondrial biogenesis and uncoupling status following βHB exposure. Conclusions: In conclusion, βHB increases mitochondrial respiration, but not ATP production, in mammalian adipocytes, indicating altered mitochondrial coupling. These findings may partly explain the increased metabolic rate evident in states of elevated ketones, and may facilitate the development of novel anti-obesity interventions.
Two-Player Game in a Complex Landscape: 26S Proteasome, PKA, and Intracellular Calcium Concentration Modulate Mammalian Sperm Capacitation by Creating an Integrated Dialogue—A Computational Analysis
Authors: Taraschi Angela , Cimini Costanza , Capacchietti Giulia , Ramal-Sanchez Marina , Valbonetti Luca , Machado-Simoes Juliana , Moussa Fadl , Tagaram Israiel , Mokh Samia , Al Iskandarani Mohamad , Colosimo Alessia , Barboni Barbara , Bernabò Nicola
Published: August 2020

Recent experimental findings suggest the involvement of the 26S proteasome, the main protease active in eukaryotic cells, in the process that leads mammalian sperm to become fully fertile, so-called capacitation. Unfortunately, its role in male gametes signaling is still far from being completely understood. For this reason, here, we realized a computational model, based on network theory, with the aim of rebuilding and exploring its signaling cascade. As a result, we found that the 26S proteasome is part of a signal transduction system that recognizes the bicarbonate ion as an input terminal and two intermediate layers of information processing. The first is under the control of the 26S proteasome and protein kinase A (PKA), which are strongly interconnected, while the latter depends on intracellular calcium concentrations. Both are active in modulating sperm function by influencing the protein phosphorylation pattern and then controlling several key events in sperm capacitation, such as membrane and cytoskeleton remodeling. Then, we found different clusters of molecules possibly involved in this pathway and connecting it to the immune system. In conclusion, this work adds a piece to the puzzle of protease and kinase crosstalk involved in the physiology of sperm cells.
Pathophysiology of Type 2 Diabetes Mellitus
Authors: Galicia-Garcia Unai , Benito-Vicente Asier , Jebari Shifa , Larrea-Sebal Asier , Siddiqi Haziq , Uribe Kepa B. , Ostolaza Helena , Martín César
Published: August 2020

Type 2 Diabetes Mellitus (T2DM), one of the most common metabolic disorders, is caused by a combination of two primary factors: defective insulin secretion by pancreatic β-cells and the inability of insulin-sensitive tissues to respond appropriately to insulin. Because insulin release and activity are essential processes for glucose homeostasis, the molecular mechanisms involved in the synthesis and release of insulin, as well as in its detection are tightly regulated. Defects in any of the mechanisms involved in these processes can lead to a metabolic imbalance responsible for the development of the disease. This review analyzes the key aspects of T2DM, as well as the molecular mechanisms and pathways implicated in insulin metabolism leading to T2DM and insulin resistance. For that purpose, we summarize the data gathered up until now, focusing especially on insulin synthesis, insulin release, insulin sensing and on the downstream effects on individual insulin-sensitive organs. The review also covers the pathological conditions perpetuating T2DM such as nutritional factors, physical activity, gut dysbiosis and metabolic memory. Additionally, because T2DM is associated with accelerated atherosclerosis development, we review here some of the molecular mechanisms that link T2DM and insulin resistance (IR) as well as cardiovascular risk as one of the most important complications in T2DM.
Indirect Photodegradation of Sulfamethoxazole and Trimethoprim by Hydroxyl Radicals in Aquatic Environment: Mechanisms, Transformation Products and Eco-Toxicity Evaluation
Authors: Yang Jiaoxue , Lv Guochun , Zhang Chenxi , Wang Zehua , Sun Xiaomin
Published: August 2020

The bacteriostatic antibiotics, sulfamethoxazole (SMX) and trimethoprim (TMP), have frequently been found in wastewater and surface water, which raises the concerns about their ecotoxicological effects. The indirect photochemical transformation has been proven to be an efficient way to degrade SMX and TMP. In this study, the reaction mechanisms of the degradation by SMX and TMF by OH radicals were investigated by theoretical calculations. Corresponding rate constants were determined and the eco-toxicity of SMX and TMP and its degradations products were predicted using theoretical models. The results indicate that the most favorable pathways for the transformation of SMX and TMP are both •OH-addition reaction of benzene ring site with lowest Gibbs free energy barriers (6.86 and 6.21 kcal mol−1). It was found that the overall reaction rate constants of •OH-initial reaction of SMX and TMP are 1.28 × 108 M−1 s−1 and 6.21 × 108 M−1 s−1 at 298 K, respectively. When comparing the eco-toxicity of transformation products with parent SMX and TMP, it can be concluded that the acute and chronic toxicities of the degraded products are reduced, but some products remain harmful for organisms, especially for daphnid (toxic or very toxic level). This study can give greater insight into the degradation of SMX and TMP by •OH through theoretical calculations in aquatic environment.
The Synthetic Cannabinoids THJ-2201 and 5F-PB22 Enhance In Vitro CB1 Receptor-Mediated Neuronal Differentiation at Biologically Relevant Concentrations
Authors: Alexandre João , Malheiro Rui , Dias da Silva Diana , Carmo Helena , Carvalho Félix , Silva João Pedro
Published: August 2020

Recreational use of synthetic cannabinoids (SCs) before and during pregnancy poses a major public health risk, due to the potential onset of neurodevelopmental disorders in the offspring. Herein, we report the assessment of the neurotoxic potential of two commonly abused SCs, THJ-2201 and 5F-PB22, particularly focusing on how they affect neuronal differentiation in vitro. Differentiation ratios, total neurite length, and neuronal marker expression were assessed in NG108-15 neuroblastoma x glioma cells exposed to the SCs at non-toxic, biologically relevant concentrations (≤1 μM), either in acute or repeated exposure settings. Both SCs enhanced differentiation ratios and total neurite length of NG108-15 cells near two-fold compared to vehicle-treated cells, in a CB1R activation-dependent way, as the CB1R blockade with a specific antagonist (SR141718) abrogated SC-induced effects. Interestingly, repeated 5F-PB22 exposure was required to reach effects similar to a single THJ-2201 dose. Cell viability and proliferation, mitochondrial membrane potential, and intracellular ATP levels were also determined. The tested SCs increased mitochondrial tetramethyl rhodamine ethyl ester (TMRE) accumulation after 24 h at biologically relevant concentrations but did not affect any of the other toxicological parameters. Overall, we report firsthand the CB1R-mediated enhancement of neurodifferentiation by 5F-PB22 and THJ-2201 at biologically relevant concentrations.
Exploring Serum Transferrin Regulation of Nonferric Metal Therapeutic Function and Toxicity
Authors: Benjamín-Rivera Josué A. , Cardona-Rivera Andrés E. , Vázquez-Maldonado Ángel L. , Dones-Lassalle Christian Y. , Pabón-Colon Héctor L. , Rodríguez-Rivera Héctor M. , Rodríguez Israel , González-Espiet Jean C. , Pazol Jessika , Pérez-Ríos Jobaniel D. , Catala-Torres José F. , Carrasquillo Rivera Marielie , De Jesus-Soto Michael G. , Cordero-Virella Nicolle A. , Cruz-Maldonado Paola M. , González-Pagan Patricia , Hernández-Ríos Raul , Gaur Kavita , Loza-Rosas Sergio A. , Tinoco Arthur D.
Published: August 2020

Serum transferrin (sTf) plays a pivotal role in regulating iron biodistribution and homeostasis within the body. The molecular details of sTf Fe(III) binding blood transport, and cellular delivery through transferrin receptor-mediated endocytosis are generally well-understood. Emerging interest exists in exploring sTf complexation of nonferric metals as it facilitates the therapeutic potential and toxicity of several of them. This review explores recent X-ray structural and physiologically relevant metal speciation studies to understand how sTf partakes in the bioactivity of key non-redox active hard Lewis acidic metals. It challenges preconceived notions of sTf structure function correlations that were based exclusively on the Fe(III) model by revealing distinct coordination modalities that nonferric metal ions can adopt and different modes of binding to metal-free and Fe(III)-bound sTf that can directly influence how they enter into cells and, ultimately, how they may impact human health. This knowledge informs on biomedical strategies to engineer sTf as a delivery vehicle for metal-based diagnostic and therapeutic agents in the cancer field. It is the intention of this work to open new avenues for characterizing the functionality and medical utility of nonferric-bound sTf and to expand the significance of this protein in the context of bioinorganic chemistry.
Comparison of Changes in the Condylar Volume and Morphology in Skeletal Class III Deformities Undergoing Orthognathic Surgery Using a Customized versus Conventional Miniplate: A Retrospective Analysis
Authors: Lim You Na , Park In-Young , Kim Jong-Cheol , Byun Soo-Hwan , Yang Byoung-Eun
Published: August 2020

With the great leap in the development of three-dimensional computer-assisted surgical technology, surgeons can use a variety of assistive methods to achieve better results and evaluate surgical outcomes in detail. This retrospective study aimed to evaluate the postoperative stability after bilateral sagittal split ramus osteotomy by volume rendering methods and to evaluate how postoperative stability differs depending on the type of surgical plate. Of the patients who underwent BSSRO, ten patients in each group (non-customized miniplate and customized miniplate) who met the inclusion criteria were selected. Preoperative and postoperative cone-beam computed tomography data were collected, and condylar morphological and landmark measurements were obtained using Checkpoint and OnDemand software, respectively. The postoperative condylar morphological dataset revealed no significant difference (p > 0.05) between the two groups. No significant difference (p > 0.05) was observed between the two groups in horizontal, vertical, or angular landmark measurements used to quantify operational stability. These results indicate that there is no difference in the surgical outcome between the patient-specific system and the conventional method, which will allow clinicians to take advantage of the patient-specific system for this surgical procedure, with favorable results, as with the conventional method.
Whole-Body Cryotherapy Is an Effective Method of Reducing Abdominal Obesity in Menopausal Women with Metabolic Syndrome
Authors: Wiecek Magdalena , Szymura Jadwiga , Sproull Justyna , Szygula Zbigniew
Published: August 2020

Abdominal obesity predominates in menopausal women (MW) and contributes to the development of metabolic syndrome (MetS). It is associated with increased mortality related to cardiovascular disease, diabetes and fatty liver disease. The effects of whole-body cryotherapy (WBC) on body composition and the blood concentration of irisin, interleukin-6 (IL-6) and C-reactive proteins (CRP) in MW with MetS and in healthy women (HW), were assessed. The study included 19 women with MetS (61.53 ± 3.99 y, BMI 30.09 ± 4.98 kg/m2) and 18 HW (60.28 ± 3.63 y, BMI 25.50 ± 2.37 kg/m2) who were subjected to 20 WBC treatments at −130 °C for 3 min daily. In both groups, body mass (BM), BMI, abdominal circumference, triceps skinfold, total fat mass and percentage of leg fat significantly decreased after 20 WBC sessions. Additionally, the percentage of total, trunk and android fat in the MetS group were significantly decreased after 20 WBC applications. Waist circumference (WC) and waist-to-height ratio (WHtR) significantly decreased in both groups, and in the HW group, hip circumference and abdominal skinfold also significantly decreased after 10 WBC and 20 WBC treatments. In both groups, the concentration of plasma irisin significantly increased after 1 WBC and 10 WBC exposures, while the concentration of IL-6 significantly increased only in MetS group after 10 WBC and 20 WBC, and were significantly higher than in HW. CRP concentrations were significantly higher in the MetS group than in HW before 1 WBC, after 1 WBC and 10 WBC sessions, but not after 20. In the MetS group, there were significant negative correlations between the change in irisin level and the changes in WC and BM, and between the level of irisin and the change in percentage of total fat, and significant negative correlations between the change in IL-6 level and changes in WC, waist-to-hip ratio and WHtR. Whole-body cryotherapy, assuming the application of 20 treatments in the series, reduces abdominal obesity in menopausal women indirectly through the secretion of irisin and IL-6, and can be used as adjunctive therapy in the treatment of metabolic syndrome. Our conclusion is limited to menopausal women with low–moderate physical activity for whom its level as well as diet were not changed during the treatment.
Mast Cells, microRNAs and Others: The Role of Translational Research on Colorectal Cancer in the Forthcoming Era of Precision Medicine
Authors: Sammarco Giuseppe , Gallo Gaetano , Vescio Giuseppina , Picciariello Arcangelo , De Paola Gilda , Trompetto Mario , Currò Giuseppe , Ammendola Michele
Published: September 2020

Colorectal cancer (CRC) is a heterogeneous disease, molecularly and anatomically, that develops in a multi-step process requiring the accumulation of several genetic or epigenetic mutations that lead to the gradual transformation of normal mucosa into cancer. In fact, tumorigenesis is extremely complex, with many immunologic and non-immunologic factors present in the tumor microenvironment that can influence tumorigenesis. In the last few years, a role for mast cells (MCs), microRNAs (miRNAs), Kirsten rat sarcoma (KRAS) and v-raf murine sarcoma viral oncogene homologue B (BRAF) in cancer development and progression has been suggested, and numerous efforts have been made to thoroughly assess their correlation with CRC to improve patient survival and quality of life. The identification of easily measurable, non-invasive and cost-effective biomarkers, the so-called “ideal biomarkers”, for CRC screening and treatment remains a high priority. The aim of this review is to discuss the emerging role of mast cells (MCs), microRNAs (miRNAs), KRAS and BRAF as diagnostic and prognostic biomarkers for CRC, evaluating their influence as potential therapy targets in the forthcoming era of precision medicine.
Design and Validation of a Modified Compression-After-Impact Testing Device for Thin-Walled Composite Plates
Authors: Linke Markus , Flügge Felix , Olivares-Ferrer Aurelio Jose
Published: August 2020

Thin-walled fibre reinforced composites like carbon fibre reinforced plastics are very susceptible to strength reductions due to low-velocity impact damages. In aerospace engineering, the dominating failure mechanisms of impact damaged composite structures are usually investigated based on the compression after impact (CAI) test procedure. This enables the determination of the influence of impact damages on the static residual compressive strength. CAI testing procedures are typically applicable to composite plates with thicknesses larger than 3–4 mm. If thinner panels are used, they typically fail near one of the loaded edges of the CAI device, in particular the area of the free edge (which is needed for compressing the panel) and not within the free measuring area. As a consequence, the investigated samples cannot be used as valid tests for the evaluation of the residual strength in CAI testing. In order to enable an investigation of the residual strength of thin-walled plates in CAI testing, a CAI testing device is developed based on an available CAI fixture and a standardized one. For comparability reasons, this new device exhibits the same dimensions as standardized fixtures. It shows a significant improvement with respect to standardized devices concerning the measurement of mechanical behaviour during CAI testing.
COVID-19 Surveillance and Competition in Sport: Utilizing Sport Science to Protect Athletes and Staff during and after the Pandemic
Authors: Hagen Joshua , Stone Jason D. , Hornsby W. Guy , Stephenson Mark , Mangine Robert , Joseph Michael , Galster Scott
Published: September 2020

The ongoing Coronavirus 2 (COVID-19) pandemic abruptly halted athletic competition and standard training practices, consequently generating great confusion surrounding when and how to safely reintroduce sports. Therefore, tangible solutions disseminated to performance staff, coaches, and athletes are warranted to ensure optimal levels of health and physical performance for all personnel during both the current social distancing standards as well as the impending return of competition despite continued risks. In this commentary, we offer strategies for utilizing technology and data tools as components of longitudinal COVID-19 surveillance based on ongoing research efforts as well as current guidance from governing bodies, while also serving the performance needs of the athletes and staff. Recommended data sources include digital symptom and well-being surveys, standardized and routine physical performance testing, sleep and sleep physiology monitoring, cognitive applications, and temperature. This system is flexible to numerous commercially available products and is designed for easy implementation that permits instant feedback provided directly to the athlete as well as their support staff for early intervention, ultimately mitigating COVID-19 risks. We will discuss multiple options, including examples of data, data visualizations and recommendations for data interpretation and communication.
Marching Cubes and Histogram Pyramids for 3D Medical Visualization
Authors: Visutsak Porawat
Published: September 2020

This paper aims to implement histogram pyramids with marching cubes method for 3D medical volumetric rendering. The histogram pyramids are used for feature extraction by segmenting the image into the hierarchical order like the pyramid shape. The histogram pyramids can decrease the number of sparse matrixes that will occur during voxel manipulation. The important feature of the histogram pyramids is the direction of segments in the image. Then this feature will be used for connecting pixels (2D) to form up voxel (3D) during marching cubes implementation. The proposed method is fast and easy to implement and it also produces a smooth result (compared to the traditional marching cubes technique). The experimental results show the time consuming for generating 3D model can be reduced by 15.59% in average. The paper also shows the comparison between the surface rendering using the traditional marching cubes and the marching cubes with histogram pyramids. Therefore, for the volumetric rendering such as 3D medical models and terrains where a large number of lookups in 3D grids are performed, this method is a particularly good choice for generating the smooth surface of 3D object.
A Review on Strain Gradient Plasticity Approaches in Simulation of Manufacturing Processes
Authors: Russo Raffaele , Girot Mata Franck Andrés , Forest Samuel , Jacquin Dimitri
Published: September 2020

Predicting the performances of a manufactured part is extremely important, especially for industries in which there is almost no room for uncertainties, such as aeronautical or automotive. Simulations performed by means of numerical methods such as Finite Element Methods represent a powerful instrument in achieving high level of predictability. However, some particular combinations of manufactured materials and manufacturing processes might lead to unfavorable conditions in which the classical mathematical models used to predict the behavior of the continuum are not anymore able to deliver predictions that are in good agreement with experimental evidence. Since the first evidences of the shortcomings of the classical model were highlighted, many non-classical continuum mechanics theories have been developed, and most of them introduce dependencies at different levels with the Plastic Strain Gradient. This manuscript aims at gathering the milestone contributions among the Strain Gradient Plasticity Theories developed so far, with the object of exploring the way they interface with the requirements posed by the challenges in simulating manufacturing operations. Finally, the most relevant examples of the applications of Strain Gradient Plasticity Theories for manufacturing simulations have been reported from literature.
Effects of mrpigG on Development and Secondary Metabolism of Monascus ruber M7
Authors: Li Li , Chen Fusheng
Published: August 2020

Monascus pigments (MPs) have been used as food colorants for several centuries in Asian countries and are now used throughout the world via Asian catering. The MP biosynthetic pathway has been well-illustrated, but the functions of a few genes, including mrpigG, in the MP gene cluster are still unclear. In the current study, in order to investigate the function of mrpigG in M. ruber M7, gene deletion (ΔmrpigG), complementation (ΔmrpigG::mrpigG) and overexpression (M7::PtrpC-mrpigG) mutants were successfully obtained. The morphologies and biomasses, as well as the MP and citrinin production, of these mutants were analyzed. The results revealed that the disruption, complementation and overexpression of mrpigG showed no apparent defects in morphology, biomass or citrinin production (except MP production) in ΔmrpigG compared with M. ruber M7. Although the MP profiles of ΔmrpigG and M. ruber M7 were almost the same—with both having four yellow pigments, two orange pigments (OPs) and two red pigments (RPs)—their yields were decreased in ΔmrpigG to a certain extent. Particularly, the content of rubropunctatin (an OP) and its derivative rubropunctamine (an RP) in ΔmrpigG, both of which have a five-carbon side chain, accounted for 57.7%, and 22.3% of those in M. ruber M7. On the other hand, monascorubrin (an OP) and its derivative monascorubramine (an RP), both of which have a seven-carbon side chain, were increased by 1.15 and 2.55 times, respectively, in ΔmrpigG compared with M. ruber M7. These results suggest that the MrPigG protein may preferentially catalyze the biosynthesis of MPs with a five-carbon side chain.
Sporulation in Ashbya gossypii
Authors: Wendland Jürgen
Published: August 2020

Ashbya gossypii is a filamentous ascomycete belonging to the yeast family of Saccharomycetaceae. At the end of its growth phase Ashbya generates abundant amounts of riboflavin and spores that form within sporangia derived from fragmented cellular compartments of hyphae. The length of spores differs within species of the genus. Needle-shaped Ashbya spores aggregate via terminal filaments. A. gossypii is a homothallic fungus which may possess a and α mating types. However, the solo-MATa type strain is self-fertile and sporulates abundantly apparently without the need of prior mating. The central components required for the regulation of sporulation, encoded by IME1, IME2, IME4, KAR4, are conserved with Saccharomyces cerevisiae. Nutrient depletion generates a strong positive signal for sporulation via the cAMP-PKA pathway and SOK2, which is also essential for sporulation. Strong inhibitors of sporulation besides mutations in the central regulatory genes are the addition of exogenous cAMP or the overexpression of the mating type gene MATα2. Sporulation has been dissected using gene-function analyses and global RNA-seq transcriptomics. This revealed a role of Msn2/4, another potential PKA-target, for spore wall formation and a key dual role of the protein A kinase Tpk2 at the onset of sporulation as well as for breaking the dormancy of spores to initiate germination. Recent work has provided an overview of ascus development, regulation of sporulation and spore maturation. This will be summarized in the current review with a focus on the central regulatory genes. Current research and open questions will also be discussed.
Understanding the Importance of Dynamic Landscape Connectivity
Authors: Zeller Katherine , Lewison Rebecca , Fletcher Robert , Tulbure Mirela , Jennings Megan
Published: August 2020

Landscape connectivity is increasingly promoted as a conservation tool to combat the negative effects of habitat loss, fragmentation, and climate change. Given its importance as a key conservation strategy, connectivity science is a rapidly growing discipline. However, most landscape connectivity models consider connectivity for only a single snapshot in time, despite the widespread recognition that landscapes and ecological processes are dynamic. In this paper, we discuss the emergence of dynamic connectivity and the importance of including dynamism in connectivity models and assessments. We outline dynamic processes for both structural and functional connectivity at multiple spatiotemporal scales and provide examples of modeling approaches at each of these scales. We highlight the unique challenges that accompany the adoption of dynamic connectivity for conservation management and planning in the context of traditional conservation prioritization approaches. With the increased availability of time series and species movement data, computational capacity, and an expanding number of empirical examples in the literature, incorporating dynamic processes into connectivity models is more feasible than ever. Here, we articulate how dynamism is an intrinsic component of connectivity and integral to the future of connectivity science.
Sub-Surface Carbon Stocks in Northern Taiga Landscapes Exposed in the Batagay Megaslump, Yana Upland, Yakutia
Authors: Shepelev Andrei G. , Kizyakov Alexander , Wetterich Sebastian , Cherepanova Alexandra , Fedorov Alexander , Syromyatnikov Igor , Savvinov Grigoriy
Published: August 2020

The most massive and fast-eroding thaw slump of the Northern Hemisphere located in the Yana Uplands of Northern Yakutia was investigated to assess in detail the cryogenic inventory and carbon pools of two distinctive Ice Complex stratigraphic units and the uppermost cover deposits. Differentiating into modern and Holocene near-surface layers (active layer and shielding layer), highest total carbon contents were found in the active layer (18.72 kg m−2), while the shielding layer yielded a much lower carbon content of 1.81 kg m−2. The late Pleistocene upper Ice Complex contained 10.34 kg m−2 total carbon, and the mid-Pleistocene lower Ice Complex 17.66 kg m−2. The proportion of organic carbon from total carbon content is well above 70% in all studied units with 94% in the active layer, 73% in the shielding layer, 83% in the upper Ice Complex and 79% in the lower Ice Complex. Inorganic carbon is low in the overall structure of the deposits.
Improving Phylogenetic Signals of Mitochondrial Genes Using a New Method of Codon Degeneration
Authors: Xia Xuhua
Published: August 2020

Recovering deep phylogeny is challenging with animal mitochondrial genes because of their rapid evolution. Codon degeneration decreases the phylogenetic noise and bias by aiming to achieve two objectives: (1) alleviate the bias associated with nucleotide composition, which may lead to homoplasy and long-branch attraction, and (2) reduce differences in the phylogenetic results between nucleotide-based and amino acid (AA)-based analyses. The discrepancy between nucleotide-based analysis and AA-based analysis is partially caused by some synonymous codons that differ more from each other at the nucleotide level than from some nonsynonymous codons, e.g., Leu codon TTR in the standard genetic code is more similar to Phe codon TTY than to synonymous CTN codons. Thus, nucleotide similarity conflicts with AA similarity. There are many such examples involving other codon families in various mitochondrial genetic codes. Proper codon degeneration will make synonymous codons more similar to each other at the nucleotide level than they are to nonsynonymous codons. Here, I illustrate a “principled” codon degeneration method that achieves these objectives. The method was applied to resolving the mammalian basal lineage and phylogenetic position of rheas among ratites. The codon degeneration method was implemented in the user-friendly and freely available DAMBE software for all known genetic codes (genetic codes 1 to 33).
Material Parameters Identification of Historic Lighthouse Based on Operational Modal Analysis
Authors: Tomaszewska Agnieszka , Drozdowska Milena , Szafrański Marek
Published: August 2020

In the present paper, the identification of the material parameters of a masonry lighthouse is discussed. A fully non-invasive method was selected, in which the material properties were determined via numerical model validation applied to the first pair of natural frequencies and their related mode shapes, determined experimentally. The exact structural model was built by means of the finite element method. To obtain experimental data for the inverse analysis, operational modal analysis was applied to the structure. Three methods were considered: peak picking (PP), eigensystem realization algorithm (ERA) and natural excitation technique with ERA (NExT-ERA). The acceleration’s responses to environmental excitations, enhanced in some periods of time by sheet piling hammering or by sudden interruptions like wind stroke, were assumed within the analysis input. Different combinations of the input were considered in the PP and NExT-ERA analysis to find the most reasonable modal forms. A number of time periods of a free-decay character were considered in the ERA technique to finally calculate the averaged modal forms. Finally, the elastic modulus, Poisson’s ratio and material density of brick, sandstone and granite masonry were determined. The obtained values supplement the state of the art database concerning historic building materials. In addition, the numerical model obtained in the analysis may be used in further cases of structural analysis.