bluuebunny/crossref_metadata_2025 huggingface dataset:
Single stage surgical treatment of amniotic band syndrome: Case report
Authors: Komarcevic Aleksandar , Pajic Milos , Djermanov Marina , Jovanovic Mladen , Tatic Milanka
Published: November 2016

Introduction. Amniotic band syndrome is a rare congenital disorder with
clinical presentation of constricting bands in different parts of extremities
or whole extremities. Conservative or surgical treatment is provided
depending on the type and severity of the anomaly. Case Outline. The paper
presents the case of a neonate patient with constriction bands localized on
the left leg. During the second week of life, a surgery was indicated, and a
single-stage multiple Z-plasty was performed to correct the anomalies on the
left lower leg. Postoperative edema in the distal part of the lower leg was
easily managed by incisions and drainage. Two months later, the correction of
the stricture of the left thigh was managed using the same procedure. The
postoperative course was uneventful and the outcome was satisfactory after a
two-year follow-up. Conclusion. Evaluation of a patient with amniotic band
syndrome, as well as diagnosis, monitoring, treatment and postoperative care,
should always be multidisciplinary. A single-stage correction approach
provided satisfactory both functional and aesthetic results. Given many
morphological variations of the syndrome, a decision on the strategy of
treatment should be made individually for each patient.
Vojislav Arnovljevic described "Sézary syndrome" ten years before Sézary and Bouvrain
Authors: Colovic Natasa , Jankovic Marko , Lekovic Danijela , Gotic Mirjana
Published: November 2016

In 1938 Sezary and Bouvrain reported on a patient with a set of symptoms
which later began to carry an eponymous designation ?Sezary syndrome.? Ten
years previously, Vojislav Arnovljevic had described a patient with exactly
the same set of symptoms, as well as physical, laboratory, autopsy, and
histopathology findings. Unfortunately, his contribution remained unnoticed,
not only by the international but Serbian audience as well. In 1928, in the
Serbian Archives of Medicine, in Serbian, Vojislav Arnovljevic published an
article titled ?The chronic lymphoid leukaemia with skin lymphomatosis,? in
which he described a 43-year-old man with a two-year history of progressive
development of a diffuse erythroderma with itching and hair loss over the
entire torso, leukemia of 240,000/mm3, 91% of which lymphocytes and 5%
eosinophils, who soon after admission developed a bronchopulmonary infection
and died. The autopsy showed a pronounced lymphadenopathy in axillae, chest,
and abdomen, enlarged liver and spleen with multiple infiltrates and thick
skin. The histology confirmed a profound lymphocyte infiltration of axillar,
mediastinal and abdominal lymph nodes, as well as liver, spleen and skin,
while ?the reaction of the other parts of the lymph and blood systems was
relatively weak.? There is more than enough clinical, laboratory, autopsy and
histological evidence to support that the patient Arnovljevic described in
1928 had a syndrome that ten years later was described by Sezary and
Bouvrain, which now bears the eponymous designation of Sezary syndrome.
Outcome in cats with benign ureteral obstructions treated by means of ureteral stenting versus ureterotomy
Authors: Culp William T. N. , Palm Carrie A. , Hsueh Christine , Mayhew Philipp D. , Hunt Geraldine B. , Johnson Eric G. , Drobatz Kenneth J.
Published: November 2016

OBJECTIVE To evaluate the outcome for cats with benign ureteral obstructions treated by means of ureteral stenting and to compare the outcome for these cats with outcome for a historical cohort of cats treated by means of ureterotomy only.

DESIGN Prospective study with historical cohort.

ANIMALS 62 client-owned cats with benign ureteral obstructions, including 26 cats treated with ureteral stenting and 36 cats previously treated with ureterotomy.

PROCEDURES Data were recorded prospectively (ureteral stent cases) or collected retrospectively from the medical records (ureterotomy cases), and results were compared.

RESULTS Cats treated with ureteral stents had significantly greater decreases in BUN and serum creatinine concentrations 1 day after surgery and at hospital discharge, compared with values for cats that underwent ureterotomy. Six cats in the ureteral stent group developed abdominal effusion after surgery, and cats in this group were significantly more likely to develop abdominal effusion when a ureterotomy was performed than when it was not. Cats that developed abdominal effusion after surgery were significantly less likely to survive to hospital discharge. Cats that underwent ureteral stenting were significantly more likely to have resolution of azotemia prior to hospital discharge than were cats that underwent ureterotomy alone.

CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that cats with benign ureteral obstructions treated with ureteral stenting were more likely to have resolution of azotemia prior to hospital discharge, compared with cats undergoing ureterotomy alone. Results of ureteral stenting were encouraging, but further investigation is warranted.
Influence of Operating Parameters on Running-in Wear of EN-31 Steel
Authors: Hanief M. , Wani M. F.
Published: November 2016

In this paper, effect of operating parameters (temperature, surface roughness and load) was investigated to determine the influence of each parameter on the wear rate. A mathematical model was developed to establish a functional relationship between the running-in wear rate and the operating parameters. The proposed model being non-linear, it was linearized by logarithmic transformation and the optimal values of model parameters were obtained by least square method. It was found that the surface roughness has significant effect on wear rate followed by load and temperature. The adequacy of the model was estimated by statistical methods (coefficient of determination (R2) and mean absolute percentage error (MAPE)) .
Implementation of Biogas Stations into Smart Heating and Cooling Network
Authors: Milčák P. , Konvička J. , Jasenská M.
Published: November 2016

The paper is aimed at the description of implementation of a biogas station into software environment for the „Smart Heating and Cooling Networks”. The aim of this project is creation of a software tool for preparation of operation and optimization of treatment of heat/cool in small regions. In this case, the biogas station represents a kind of renewable energy source, which, however, has its own operational specifics which need to be taken into account at the creation of an implementation project. For a specific biogas station, a detailed computational model was elaborated, which is parameterized in particular for an optimization of the total computational time.
Failure of Itraconazole to Prevent T-Helper Type 2 Cell Immune Deviation: Implications for Chronic Rhinosinusitis
Authors: Kennedy Joshua L. , Steinke John W. , Liu Lixia , Negri Julie , Borish Larry , Payne Spencer C.
Published: November 2016

Background

T-helper (Th) type 2 cell inflammation is the hallmark of several disease processes, including asthma, atopic dermatitis, and some forms of chronic rhinosinusitis. Itraconazole has been used as both an antifungal and an anti-inflammatory agent, with some success in many of these diseases, in part, by altering Th2 cytokine expression by T cells. It is not known whether this merely reflects inhibition of established Th2-like cells or the inhibition of differentiation of naive T cells into Th2-like cells.

Objective

To evaluate the role of itraconazole in the differentiation of naive T cells during activation.

Methods

Naive CD45RA+ T cells were isolated from peripheral blood mononuclear cells from healthy volunteers. Th1 and Th2 type cells were differentiated in the presence of varying concentrations of itraconazole. After stimulation with anti-CD3 and anti-CD28 beads, carboxyfluorescein succinimidyl ester dilution was performed to evaluate proliferation and intracellular cytokine staining for interleukin (IL) 4 and interferon (IFN) gamma within proliferating T cells was measured along with enzyme-linked immunosorbent assay for secreted IL-5, IL-13, and IFN gamma.

Results

Itraconazole had no effect on proliferation of unbiased, Th1, or Th2 cells. Similarly, there was no effect of itraconazole on either intracellular cytokine staining of IL-4 and IFN gamma or secreted cytokine expression of IFN gamma, IL-5, and IL-13 in any of the cell populations.

Conclusion

Itraconazole did not alter the ability of naive T cells to proliferate or secrete cytokines under Th1 or Th2 deviating conditions in vitro. As such, reported inhibition of Th2-like lymphocyte function by itraconazole reflected action on mature effector cells and may have underscored why antifungal treatment failed in many clinical trials of eosinophilic chronic rhinosinusitis.
Role of Lumbar Drains in Contemporary Endonasal Skull Base Surgery: Meta-Analysis and Systematic Review
Authors: D'Anza Brian , Tien Due , Stokken Janalee K. , Recinos Pablo F. , Woodard Troy R. , Sindwani Raj
Published: November 2016

Background

Historically, lumbar drains (LD) have played a prominent role in endonasal skull base surgery. Over the past few decades, advancements in techniques have augmented our ability to successfully reconstruct complex skull base defects and often obviates the need for cerebrospinal fluid (CSF) diversion. Clarity on the appropriate use of LDs is needed.

Objective

To examine the literature for the need for LDs in contemporary skull base reconstruction after resection of skull base tumors.

Methods

A systematic literature review of English language articles by using PubMed and Ovid. Search terms included “lumbar drain,” “CSF leak,” and “endoscopic endonasal reconstruction.” Articles were included when they pertained to adults, used current methods for reconstruction (i.e., multilayered repair or vascularized tissue), and addressed CSF leak rates secondary to endoscopic resection of skull base masses. All the studies discussed CSF leaks that resulted from traumatic-, idiopathic-, or sinus surgery-related iatrogenic causes were excluded. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed.

Results

A total of five articles met inclusion criteria. These comprised 376 endoscopic skull base tumor resection and reconstruction cases; 5.59% developed postoperative CSF leaks. A meta-analysis was conducted by incorporating three studies that met criteria based on testing for heterogeneity. The average odds ratio for postoperative CSF leak for patients who did not have an LD relative to patients who had an LD was 0.590 (95% confidence interval, 0.214–1.630). Given a p value of 0.30, the results demonstrated a lack of statistically significant improvement between patients who had an LD and patients who did not have an LD. Various details, such as the defect size and type of CSF leak, were not consistently reported among studies.

Conclusions

Available evidence for the use of LDs in skull base surgery is of poor quality. Analysis of the literature revealed heterogenous and varied reporting in the primary literature. Further studies that include randomized controlled trials are needed.
Culture-Directed Topical Antibiotic Treatment for Chronic Rhinosinusitis
Authors: Lee Victoria S. , Davis Greg E.
Published: November 2016

Background

Topical antibiotics, delivered optimally as high-volume culture-directed sinus irrigations, are being increasingly used for recalcitrant chronic rhinosinusitis (CRS). Their impact on subjective and objective outcome measures, however, is still unclear.

Objective

To assess if the use of topical antibiotics in recalcitrant CRS is associated with improved 20-Item Sino-Nasal Outcome Test and Lund-Kennedy endoscopic scores, and to determine the negative posttreatment culture “control” rate.

Methods

Patients were included in the study if they met diagnostic criteria for CRS, received high-volume topical antibiotic sinus irrigations twice daily for 1 month, between December 2009 and May 2015, and had undergone endoscopic sinus surgery. The primary outcome was the 20-Item Sino-Nasal Outcome Test score. Secondary outcomes were the Lund-Kennedy endoscopic score and a negative posttreatment culture “control” rate. Paired t-tests were used to compare pre- and posttreatment scores. Patients with cystic fibrosis were analyzed separately.

Results

Of the 58 patients included, 47% had nasal polyposis, 57% had asthma, 16% had aspirin sensitivity, and 55% had environmental allergies. The median Lund-Mackay computed tomography score was 11 (interquartile range, 6–16), and the median time to follow-up was 8 weeks (interquartile range, 6–10 weeks). The 20-Item Sino-Nasal Outcome Test scores improved from pre- to posttreatment period, although this was not significant mean 1.5 [confidence interval {CI} 1.3, 1.7] to mean 1.3 [CI 1.1, 1.6]; p = 0.16). Lund-Kennedy endoscopic scores, however, significantly improved from pre- to posttreatment (mean 4.9 [CI 4.3, 5.6] to mean 4.1 [CI 3.5, 4.7]; p = 0.05). Of the 47 patients with complete culture data, 72% had negative posttreatment culture results, defined as “controlled.” Only one patient discontinued treatment, related to discomfort from irrigations.

Conclusion

In patients with recalcitrant CRS, the use of topical antibiotics trended toward improvement in symptom severity and significantly improved endoscopic appearance. Furthermore, 72% had negative posttreatment culture results, meaning microbiological “control.” The results of this study support the use of high-volume culture-directed topical antibiotics, and, in the future, more rigorous prospective studies are warranted.
Assessing differences in physiologic subjective response toward male and female orientated sexually explicit videos in heterosexual individuals
Authors: Landry Samantha , Goncalves Melissa K. , Kukkonen Tuuli M.
Published: November 2016

The goal of the present study was to examine sexual response to male- and female- oriented sexually explicit films in heterosexual men and women. Forty participants (20 men and 20 women; mean age=29.42 years) attended three separate lab sessions. One 15 minute sexually explicit video was shown per session. For session one, all participants viewed a female-oriented film selected by the experimenters. The films used for subsequent sessions were counterbalanced male-oriented or female-oriented clips that had been previously studied. A thermographic camera measured temperature on the penile shaft for men and labia for women. Continuous and discrete self-reported sexual arousal was also obtained. Genital temperature was averaged into 15 one-minute bins and a repeated-measures ANOVA was conducted. Men demonstrated significantly greater increases in temperature over time than women, F (14, 980)=19.27, p=.000, however there were no significant differences between films or sex × film interaction. Women reported significantly higher subjective sexual arousal to the films than men, F (1, 69) range=3.89 to 9.67, p range=.01 to .05, but there were no significant differences between films or a sex × film interaction. Results suggest that film orientation has minimal impact on physiologic sexual responsiveness in men or women. Although both sexes demonstrated significant increases in sexual arousal for these pre-selected films, future laboratory research would benefit from examining whether participant-selected stimuli produces a greater response than experimenter-selected films.
Optical Orientation and Inverse Spin Hall Effect as Effective Tools to Investigate Spin-Dependent Diffusion
Authors: Finazzi Marco , Bottegoni Federico , Zucchetti Carlo , Bollani Monica , Ballabio Andrea , Frigerio Jacopo , Rortais Fabien , Vergnaud Céline , Marty Alain , Jamet Matthieu , Isella Giovanni , Ciccacci Franco
Published: November 2016

In this work we address optical orientation, a process consisting in the excitation of spin polarized electrons across the gap of a semiconductor. We show that the combination of optical orientation with spin-dependent scattering leading to the inverse spin-Hall effect, i.e., to the conversion of a spin current into an electrical signal, represents a powerful tool to generate and detect spin currents in solids. We consider a few examples where these two phenomena together allow addressing the spin-dependent transport properties across homogeneous samples or metal/semiconductor Schottky junctions.
Improving the Stability and Accuracy of Power Hardware-in-the-Loop Simulation Using Virtual Impedance Method
Authors: Zha Xiaoming , Yin Chenxu , Sun Jianjun , Huang Meng , Li Qionglin
Published: November 2016

Power hardware-in-the-loop (PHIL) systems are advanced, real-time platforms for combined software and hardware testing. Two paramount issues in PHIL simulations are the closed-loop stability and simulation accuracy. This paper presents a virtual impedance (VI) method for PHIL simulations that improves the simulation’s stability and accuracy. Through the establishment of an impedance model for a PHIL simulation circuit, which is composed of a voltage-source converter and a simple network, the stability and accuracy of the PHIL system are analyzed. Then, the proposed VI method is implemented in a digital real-time simulator and used to correct the combined impedance in the impedance model, achieving higher stability and accuracy of the results. The validity of the VI method is verified through the PHIL simulation of two typical PHIL examples.
An Improved Adaptive-Torque-Gain MPPT Control for Direct-Driven PMSG Wind Turbines Considering Wind Farm Turbulences
Authors: Zhang Xiaolian , Huang Can , Hao Sipeng , Chen Fan , Zhai Jingjing
Published: November 2016

Maximum power point tracking (MPPT) plays an important role in increasing the efficiency of a wind energy conversion system (WECS). In this paper, three conventional MPPT methods are reviewed: power signal feedback (PSF) control, decreased torque gain (DTG) control, and adaptive torque gain (ATG) control, and their potential challenges are investigated. It is found out that the conventional MPPT method ignores the effect of wind turbine inertia and wind speed fluctuations, which lowers WECS efficiency. Accordingly, an improved adaptive torque gain (IATG) method is proposed, which customizes adaptive torque gains and enhances MPPT performances. Specifically, the IATG control considers wind farm turbulences and works out the relationship between the optimal torque gains and the wind speed characteristics, which has not been reported in the literature. The IATG control is promising, especially under the ongoing trend of building wind farms with large-scale wind turbines and at low and medium wind speed sites.
Experimental Investigation of Thermal Behaviors in Window Systems by Monitoring of Surface Condensation Using Full-Scale Measurements and Simulation Tools
Authors: Hong Goopyo , Kim Daeung , Kim Byungseon
Published: November 2016

The aim of the present study was to investigate the thermal performance of window systems using full-scale measurements and simulation tools. A chamber was installed on the balcony of an apartment to control the temperatures which can create condensation on the interior surfaces of window systems. The condensation process on the window was carefully scrutinized when outdoor and indoor temperature and indoor relative humidity ranged from −15 °C to −20 °C, 23 °C to 24 °C, and 50% to 65%, respectively. The results of these investigations were analyzed to determine how the moisture is influenced by changing temperatures. It appears that the glass-edge was highly susceptible to the temperature variations and the lowest temperature on the glass edge was caused by the heat transfer through the spacer, between the two glass panels of the window. The results from the simulation used in this study confirm that the thermal performance of window systems can be improved the use of super insulated or thermally broken spacers. If the values of the indoor humidity and temperature are given, then the outdoor temperature when condensation forms can be obtained by using Temperature Difference Ratio (TDR). This methodology can be employed to predict the possible occurrence of condensation.
Nutrient Resorption and Phenolics Concentration Associated with Leaf Senescence of the Subtropical Mangrove Aegiceras corniculatum: Implications for Nutrient Conservation
Authors: Chen Hui , Xu Benbo , Wei Shudong , Zhang Lihua , Zhou Haichao , Lin Yiming
Published: November 2016

Aegiceras corniculatum (L.) Blanco, a mangrove shrub species in the Myrsine family, often grows at the seaward edge of the mangrove zone in China. In the present study, seasonal dynamics of nutrient resorption and phenolics concentration associated with leaf senescence of A. corniculatum were investigated in order to evaluate its possible nutrient conservation strategies in the subtropical Zhangjiang river estuary. It was found that the nitrogen (N) and phosphorus (P) concentrations in mature leaves showed similar seasonal changes with the highest concentrations in winter and the lowest in summer, and were significantly higher than those in senescent leaves. The N:P ratios of mature leaves through the year were found to be less than 14, indicating that the A. corniculatum forest was N-limited. The nitrogen resorption efficiency (NRE) was higher than phosphorus resorption efficiency (PRE), and N resorption was complete. In addition, A. corniculatum leaves contained high total phenolics (TPs) and total condensed tannin (TCT) levels (both above 20%). TPs concentrations in mature and senescent leaves were all inversely related to their N or P concentrations. TPs:N and TCT:N ratios in senescent leaves were significantly higher than those in mature leaves. The obtained results suggested that high NRE during leaf senescence and high TPs:N and TCT:N ratios in senescent leaves might be important nutrient conservation strategies for the mangrove shrub A. corniculatum forest growing in N-limited conditions.
Modeling the Link between Left Ventricular Flow and Thromboembolic Risk Using Lagrangian Coherent Structures
Authors: May-Newman Karen , Vu Vi , Herold Brian
Published: November 2016

A thrombus is a blood clot that forms on a surface, and can grow and detach, presenting a high risk for stroke and pulmonary embolism. This risk increases with blood-contacting medical devices, due to the immunological response to foreign surfaces and altered flow patterns that activate the blood and promote thromboembolism (TE). Abnormal blood transport, including vortex behavior and regional stasis, can be assessed from Lagrangian Coherent Structures (LCS). LCS are flow structures that bound transport within a flow field and divide the flow into regions with maximally attracting/repelling surfaces that maximize local shear. LCS can be identified from finite time Lyapunov exponent (FTLE) fields, which are computed from velocity field data. In this study, the goal was to use FTLE analysis to evaluate LCS in the left ventricle (LV) using velocity data obtained from flow visualization of a mock circulatory loop. A model of dilated cardiomyopathy (DCM) was used to investigate the effect of left ventricular assist device (LVAD) support on diastolic filling and transport in the LV. A small thrombus in the left ventricular outflow tract was also considered using data from a corresponding LV model. The DCM LV exhibited a direct flow of 0.8 L/cardiac cycle, which was tripled during LVAD support Delayed ejection flow was doubled, further illustrating the impact of LVAD support on blood transport. An examination of the attracting LCS ridges during diastolic filling showed that the increase is due primarily to augmentation of A wave inflow, which is associated with increased vortex circulation, kinetic energy and Forward FTLE. The introduction of a small thrombus in the left ventricular outflow tract (LVOT) of the LV had a minimal effect on diastolic inflow, but obstructed systolic outflow leading to decreased transport compared with the unobstructed LVOT geometry. Localized FTLE in the LVOT increased dramatically with the small thrombus model, which reflects greater recirculation distal to the thrombus location. The combination of the thrombus and the LVAD increased stasis distal to the thrombus, increasing the likelihood of recurring coagulation during Series flow conditions. The extension of the results of the previous studies with this analysis provides a more sensitive indicator of TE risk than the Eulerian velocity values do, and may provide an important tool for evaluating medical device design, surgical implantation, and treatment options.
Screening for Mutations in the TBX1 Gene on Chromosome 22q11.2 in Schizophrenia
Authors: Ping Lieh-Yung , Chuang Yang-An , Hsu Shih-Hsin , Tsai Hsin-Yao , Cheng Min-Chih
Published: November 2016

A higher-than-expected frequency of schizophrenia in patients with 22q11.2 deletion syndrome suggests that chromosome 22q11.2 harbors the responsive genes related to the pathophysiology of schizophrenia. The TBX1 gene, which maps to the region on chromosome 22q11.2, plays a vital role in neuronal functions. Haploinsufficiency of the TBX1 gene is associated with schizophrenia endophenotype. This study aimed to investigate whether the TBX1 gene is associated with schizophrenia. We searched for mutations in the TBX1 gene in 652 patients with schizophrenia and 567 control subjects using a re-sequencing method and conducted a reporter gene assay. We identified six SNPs and 25 rare mutations with no association with schizophrenia from Taiwan. Notably, we identified two rare schizophrenia-specific mutations (c.-123G>C and c.-11delC) located at 5′ UTR of the TBX1 gene. The reporter gene assay showed that c.-123C significantly decreased promoter activity, while c.-11delC increased promoter activity compared with the wild-type. Our findings suggest that the TBX1 gene is unlikely a major susceptible gene for schizophrenia in an ethnic Chinese population for Taiwan, but a few rare mutations in the TBX1 gene may contribute to the pathogenesis of schizophrenia in some patients.
Assessing Essential Qualities of Urban Space with Emotional and Visual Data Based on GIS Technique
Authors: Li Xin , Hijazi Ihab , Koenig Reinhard , Lv Zhihan , Zhong Chen , Schmitt Gerhard
Published: November 2016

Finding a method to evaluate people’s emotional responses to urban spaces in a valid and objective way is fundamentally important for urban design practices and related policy making. Analysis of the essential qualities of urban space could be made both more effective and more accurate using innovative information techniques that have become available in the era of big data. This study introduces an integrated method based on geographical information systems (GIS) and an emotion-tracking technique to quantify the relationship between people’s emotional responses and urban space. This method can evaluate the degree to which people’s emotional responses are influenced by multiple urban characteristics such as building shapes and textures, isovist parameters, visual entropy, and visual fractals. The results indicate that urban spaces may influence people’s emotional responses through both spatial sequence arrangements and shifting scenario sequences. Emotional data were collected with body sensors and GPS devices. Spatial clustering was detected to target effective sampling locations; then, isovists were generated to extract building textures. Logistic regression and a receiver operating characteristic analysis were used to determine the key isovist parameters and the probabilities that they influenced people’s emotion. Finally, based on the results, we make some suggestions for design professionals in the field of urban space optimization.
Role of Osteogenic Growth Peptide (OGP) and OGP(10–14) in Bone Regeneration: A Review
Authors: Pigossi Suzane , Medeiros Marcell , Saska Sybele , Cirelli Joni , Scarel-Caminaga Raquel
Published: November 2016

Bone regeneration is a process that involves several molecular mediators, such as growth factors, which directly affect the proliferation, migration and differentiation of bone-related cells. The osteogenic growth peptide (OGP) and its C-terminal pentapeptide OGP(10–14) have been shown to stimulate the proliferation, differentiation, alkaline phosphatase activity and matrix mineralization of osteoblastic lineage cells. However, the exact molecular mechanisms that promote osteoblastic proliferation and differentiation are not completely understood. This review presents the main chemical characteristics of OGP and/or OGP(10–14), and also discusses the potential molecular pathways induced by these growth factors to promote proliferation and differentiation of osteoblasts. Furthermore, since these peptides have been extensively investigated for bone tissue engineering, the clinical applications of these peptides for bone regeneration are discussed.
Ziyuglycoside I Inhibits the Proliferation of MDA-MB-231 Breast Carcinoma Cells through Inducing p53-Mediated G2/M Cell Cycle Arrest and Intrinsic/Extrinsic Apoptosis
Authors: Zhu Xue , Wang Ke , Zhang Kai , Zhang Ting , Yin Yongxiang , Xu Fei
Published: November 2016

Background: Due to the aggressive clinical behavior, poor outcome, and lack of effective specific targeted therapies, triple-negative breast cancer (TNBC) has currently been recognized as one of the most malignant types of tumors. In the present study, we investigated the cytotoxic effect of ziyuglycoside I, one of the major components extracted from Chinese anti-tumor herbal Radix Sanguisorbae, on the TNBC cell line MDA-MB-231. Methods: The underlying molecular mechanism of the cytotoxic effect ziyuglycoside I on MDA-MB-231 cells was investigated with cell viability assay, flow cytometric analysis and Western blot. Results: Compared to normal mammary gland Hs 578Bst cells, treatment of ziyuglycoside I resulted in a significant growth inhibitory effect on MDA-MB-231 cells. Ziyuglycoside I induced the G2/M phase arrest and apoptosis of MDA-MB-231 cells in a dose-dependent manner. These effects were found to be partially mediated through the up-regulation of p53 and p21WAF1, elevated Bax/Bcl-2 ratio, and the activation of both intrinsic (mitochondrial-initiated) and extrinsic (Fas/FasL-initiated) apoptotic pathways. Furthermore, the p53 specific siRNA attenuated these effects. Conclusion: Our study suggested that ziyuglycoside I-triggered MDA-MB-231 cell cycle arrest and apoptosis were probably mediated by p53. This suggests that ziyuglycoside I might be a potential drug candidate for treating TNBC.
Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells
Authors: Li Xueqin , Shen Jun , Jiang Yunyao , Shen Ting , You Long , Sun Xiaobo , Xu Xudong , Hu Weicheng , Wu Haifeng , Wang Gongcheng
Published: November 2016

Chloranthalactone B (CTB), a lindenane-type sesquiterpenoid, was obtained from the Chinese medicinal herb Sarcandra glabra, which is frequently used as a remedy for inflammatory diseases. However, the anti-inflammatory mechanisms of CTB have not been fully elucidated. In this study, we investigated the molecular mechanisms underlying these effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. CTB strongly inhibited the production of nitric oxide and pro-inflammatory mediators such as prostaglandin E2, tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in RAW264.7 cells stimulated with LPS. A reverse-transcription polymerase chain reaction assay and Western blot further confirmed that CTB inhibited the expression of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, and IL-1β at the transcriptional level, and decreased the luciferase activities of activator protein (AP)-1 reporter promoters. These data suggest that inhibition occurred at the transcriptional level. In addition, CTB blocked the activation of p38 mitogen-activated protein kinase (MAPK) but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1/2. Furthermore, CTB suppressed the phosphorylation of MKK3/6 by targeting the binding sites via formation of hydrogen bonds. Our findings clearly show that CTB inhibits the production of inflammatory mediators by inhibiting the AP-1 and p38 MAPK pathways. Therefore, CTB could potentially be used as an anti-inflammatory agent.
Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea
Authors: Wang Ningyuan , Huang Yan , Li An , Jiang Hailong , Wang Jie , Li Jianzhong , Qiu Lei , Li Ka , Lu Yiming
Published: November 2016

Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in the treatment of inflammatory diseases in China for centuries. In this research, we constructed a venom gland T7 phage display library of the sea snake Hydrophis cyanocinctus to screen bioactive compounds that antagonize TNF-α and identified a novel nine-amino-acid peptide, termed hydrostatin-TL1 (H-TL1). In enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) analyses, H-TL1 inhibited the interaction between TNF-α and TNF receptor 1 (TNFR1). Further, H-TL1 attenuated the cytotoxicity of TNF-α in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. H-TL1 also decreased the mRNA expression of TNF-α/TNFR1 downstream targets and suppressed the phosphorylation of well-characterized proteins of downstream signal transduction pathways in HEK-293 cells. In vivo data demonstrated that H-TL1 protects animals against dextran sodium sulfate (DSS)-induced acute colitis and lipopolysaccharide (LPS)-induced acute shock. Given its significant anti-inflammatory activity in vitro and in vivo, H-TL1 is a potential peptide for the development of new agents to treat TNF-α-associated inflammatory diseases.
Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment
Authors: Liu Yali , Han Suying , Ding Xiangming , Li Xinmin , Zhang Lifeng , Li Wanfeng , Xu Haiyan , Li Zhexin , Qi Liwang
Published: November 2016

Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H2 treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H2 treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H2 during somatic embryogenesis.
Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations
Authors: Avery Pasco , Hunter Wayne , Hall David , Jackson Mark , Powell Charles
Published: November 2016

The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.
Laboratory Testing of Silica Sol Grout in Coal Measure Mudstones
Authors: Pan Dongjiang , Zhang Nong , Xie Zhengzheng , Feng Xiaowei , Kong Yong
Published: November 2016

The effectiveness of silica sol grout on mudstones is reported in this paper. Using X-ray diffraction (XRD), the study investigates how the silica sol grout modifies mudstone mineralogy. Micropore sizes and mechanical properties of the mudstone before and after grouting with four different materials were determined with a surface area/porosity analyser and by uniaxial compression. Tests show that, after grouting, up to 50% of the mesopore volumes can be filled with grout, the dominant pore diameter decreases from 100 nm to 10 nm, and the sealing capacity is increased. Uniaxial compression tests of silica sol grouted samples shows that their elastic modulus is 21%–38% and their uniaxial compressive strength is 16%–54% of the non-grouted samples. Peak strain, however, is greater by 150%–270%. After grouting, the sample failure mode changes from brittle to ductile. This paper provides an experimental test of anti-seepage and strengthening properties of silica sol.
The Effect of Acceptor and Donor Doping on Oxygen Vacancy Concentrations in Lead Zirconate Titanate (PZT)
Authors: Slouka Christoph , Kainz Theresa , Navickas Edvinas , Walch Gregor , Hutter Herbert , Reichmann Klaus , Fleig Jürgen
Published: November 2016

The different properties of acceptor-doped (hard) and donor-doped (soft) lead zirconate titanate (PZT) ceramics are often attributed to different amounts of oxygen vacancies introduced by the dopant. Acceptor doping is believed to cause high oxygen vacancy concentrations, while donors are expected to strongly suppress their amount. In this study, La3+ donor-doped, Fe3+ acceptor-doped and La3+/Fe3+-co-doped PZT samples were investigated by oxygen tracer exchange and electrochemical impedance spectroscopy in order to analyse the effect of doping on oxygen vacancy concentrations. Relative changes in the tracer diffusion coefficients for different doping and quantitative relations between defect concentrations allowed estimates of oxygen vacancy concentrations. Donor doping does not completely suppress the formation of oxygen vacancies; rather, it concentrates them in the grain boundary region. Acceptor doping enhances the amount of oxygen vacancies but estimates suggest that bulk concentrations are still in the ppm range, even for 1% acceptor doping. Trapped holes might thus considerably contribute to the charge balancing of the acceptor dopants. This could also be of relevance in understanding the properties of hard and soft PZT.
Friction and Wear Reduction of Eccentric Journal Bearing Made of Sn-Based Babbitt for Ore Cone Crusher
Authors: Amanov Auezhan , Ahn Byungmin , Lee Moon , Jeon Yongho , Pyun Young-Sik
Published: November 2016

An anti-friction Babbitt alloy-coated bearing made by a casting process is a journal bearing, which is used in an ore cone crusher eccentric. The main purpose of the Babbitt coated eccentric is to provide a low friction to support and guide a rotating shaft. Despite the fact that the Babbitt-coated eccentric offers a low friction coefficient and can be operated without a continuous supply of lubricant, it suffers from mining environments and short service life. In this study, an ultrasonic nanocrystalline surface modification (UNSM) technique was used to further reduce the friction coefficient, to increase the wear resistance, and to extend the service life of the Sn-based Babbitt metal. The friction and wear behavior of the Sn-based Babbitt metal was investigated using a block-on-ring tester under both dry and oil-lubricated conditions. The results of the experiments revealed that the friction and wear behavior of Sn-based Babbitt metal could be improved by the application of the UNSM technique. The friction and wear mechanisms of the specimens were explained and discussed in terms of changes in surface properties—microstructure, surface hardness, surface roughness, etc.
Residual Stress Distribution and Microstructure Evolution of AA 6061-T6 Treated by Warm Laser Peening
Authors: Huang Shu , Wang Zuowei , Sheng Jie , Agyenim-Boateng Emmanuel , Liu Muxi , Yang Xiaole , Zhou Jianzhong
Published: November 2016

The aim of this paper is to study the effects of laser peening (LP) on the residual stress distribution and microstructure evolution of AA 6061-T6 under different temperatures. A laser peening experiment was conducted on the square-shape samples by using single spot and 50% overlap shock. Three-dimensional surface morphologies of treated samples were observed. The influence of peening temperature on the distribution of compressive residual stress was analyzed. An optical microscope (OM) and a transmission electron microscope (TEM) were employed to observe the microstructure evolution of the samples before and after LP. The results indicate that, as the peening temperature increases, the micro-hardness increases first and then decreases. The LP process induces high-amplitude compressive residual stress on the surface at different temperatures even if the compressive residual stress slightly reduces with increases in temperature. The maximum compressive residual stress affected layer depth is about 0.67 mm, appearing at a temperature of 160 °C. The OM test revealed that the grain size was significantly decreased after warm laser peening (WLP) and that the average value of grain size was reduced by 50%. The TEM test shows that more dislocation tangles were produced in AA 6061-T6 after WLP; compared to the LP process, the precipitate-dislocation interaction can benefit both strength and ductility for AA 6061-T6, thus enhancing the mechanical properties of the material.
Fluid Intake of Pregnant and Breastfeeding Women in Indonesia: A Cross-Sectional Survey with a Seven-Day Fluid Specific Record
Authors: Bardosono Saptawati , Prasmusinto Damar , Hadiati Diah , Purwaka Bangun , Morin Clementine , Pohan Rizki , Sunardi Diana , Chandra Dian , Guelinckx Isabelle
Published: November 2016

During pregnancy and lactation, the adequate intake (AI) for total water intake is increased. This cross-sectional survey aimed to assess Total Fluid Intake (TFI; sum of drinking water and all other fluids) of 300 pregnant and 300 breastfeeding women in Indonesia. A seven-day fluid specific record was used to assess TFI. Mean TFI of pregnant and breastfeeding women were 2332 ± 746 mL/day and 2525 ± 843 mL/day, respectively. No significant difference in TFI between pregnancy trimesters was observed, while TFI of women breastfeeding for 12–24 months postpartum (2427 ± 955 mL/day) was lower than that of the two other groups (0–5 months: 2607 ± 754 mL/day; 6–11 months: 2538 ± 807 mL/day, respectively). Forty-two and 54% of the pregnant and breastfeeding subjects, respectively, did not reach the AI of water from fluids. These AI were actually known by only 14% and 23% of the pregnant and breastfeeding subjects. However, having the knowledge about the AI did not increase the odds of reaching the AI. Concluding that a high proportion of the pregnant and breastfeeding subjects did not reach the AI of water from fluid, it seems pertinent to further assess the fluid intake, as well as their hydration status, in other countries.
Physical and Mechanical Evaluation of Silicone-Based Double-Layer Adhesive Patch Intended for Keloids and Scar Treatment Therapy
Authors: Mikolaszek Barbara , Jamrógiewicz Marzena , Mojsiewicz-Pieńkowska Krystyna , Żebrowska Maria , Sznitowska Małgorzata , Strankowska Justyna
Published: November 2016

Growing interest in silicone elastomers for pharmaceutical purposes is due to both their beneficial material effect for scar treatment and their potential as drug carriers. Regarding their morphological structure, silicone polymers possess unique properties, which enable a wide range of applicability possibilities. The present study focused on developing a double-layer adhesive silicone film (DLASil) by evaluating its physical and mechanical properties, morphology, and stability. DLASil suitability for treatment of scars and keloids was evaluated by measurement of tensile strength, elasticity modulus, and elongation. The results indicated that mechanical and physical properties of the developed product were satisfying.
Using Risk Assessment and Management Approaches to Develop Cost-Effective and Sustainable Mine Waste Management Strategies
Authors: Karachaliou Theodora , Protonotarios Vasileios , Kaliampakos Dimitris , Menegaki Maria
Published: November 2016

Soil contamination by mine wastes is an enormous challenge for both mining companies and competent authorities. Mine wastes are usually considered a major source of impacts on human health and the environment. Thus, the prevailing strategy is to reduce or completely remove the contaminants of concern through appropriate in situ or ex situ remediation techniques. Nonetheless, today’s mine waste may become tomorrow’s ore. In order to keep this option open, however, several things need to change in the concept and practice of mine waste management. This paper presents a case study from Greece, where mine waste (mainly metallurgical slags) have been disposed of, posing excess risks to human health and the environment. Complete restoration of the site would not only be cost-prohibitive but would also eliminate any possibility of future exploitation of contained resources. Considering both the protection of human health and the environment and the storage of waste for future use, a risk assessment and management approach was adopted that allowed the selection of cost-effective measures in order to: eliminate health hazards, re-use the site for recreational purposes, and secure the opportunity for resource recovery in the future.