bluuebunny/crossref_metadata_2025 huggingface dataset:
Forest Fire Probability Mapping in Eastern Serbia: Logistic Regression versus Random Forest Method
Authors: Milanović Slobodan , Marković Nenad , Pamučar Dragan , Gigović Ljubomir , Kostić Pavle , Milanović Sladjan D.
Published: December 2020

Forest fire risk has increased globally during the previous decades. The Mediterranean region is traditionally the most at risk in Europe, but continental countries like Serbia have experienced significant economic and ecological losses due to forest fires. To prevent damage to forests and infrastructure, alongside other societal losses, it is necessary to create an effective protection system against fire, which minimizes the harmful effects. Forest fire probability mapping, as one of the basic tools in risk management, allows the allocation of resources for fire suppression, within a fire season, from zones with a lower risk to those under higher threat. Logistic regression (LR) has been used as a standard procedure in forest fire probability mapping, but in the last decade, machine learning methods such as fandom forest (RF) have become more frequent. The main goals in this study were to (i) determine the main explanatory variables for forest fire occurrence for both models, LR and RF, and (ii) map the probability of forest fire occurrence in Eastern Serbia based on LR and RF. The most important variable was drought code, followed by different anthropogenic features depending on the type of the model. The RF models demonstrated better overall predictive ability than LR models. The map produced may increase firefighting efficiency due to the early detection of forest fire and enable resources to be allocated in the eastern part of Serbia, which covers more than one-third of the country’s area.
Ecological Diversity within Rear-Edge: A Case Study from Mediterranean Quercus pyrenaica Willd.
Authors: Pérez-Luque Antonio J. , Benito Blas M. , Bonet-García Francisco J. , Zamora Regino
Published: December 2020

Understanding the ecology of populations located in the rear edge of their distribution is key to assessing the response of the species to changing environmental conditions. Here, we focus on rear-edge populations of Quercus pyrenaica in Sierra Nevada (southern Iberian Peninsula) to analyze their ecological and floristic diversity. We perform multivariate analyses using high-resolution environmental information and forest inventories to determine how environmental variables differ among oak populations, and to identify population groups based on environmental and floristic composition. We find that water availability is a key variable in explaining the distribution of Q. pyrenaica and the floristic diversity of their accompanying communities within its rear edge. Three cluster of oak populations were identified based on environmental variables. We found differences among these clusters regarding plant diversity, but not for forest attributes. A remarkable match between the populations clustering derived from analysis of environmental variables and the ordination of the populations according to species composition was found. The diversity of ecological behaviors for Q. pyrenaica populations in this rear edge are consistent with the high genetic diversity shown by populations of this oak in the Sierra Nevada. The identification of differences between oak populations within the rear-edge with respect to environmental variables can aid with planning the forest management and restoration actions, particularly considering the importance of some environmental factors in key ecological aspects.
Are People Willing to Tell Pareto White Lies? A Review and New Experimental Evidence
Authors: Cartwright Edward , Xue Lian , Brown Charlotte
Published: December 2020

We explore whether individuals are averse to telling a Pareto white lie—a lie that benefits both themselves and another. We first review and summarize the existing evidence on Pareto white lies. We find that the evidence is relatively limited and varied in its conclusions. We then present new experimental results obtained using a coin-tossing experiment. Results are provided for both the UK and China. We find evidence of willingness to tell a partial lie (i.e., inflating reports slightly) and high levels of aversion to telling a Pareto white lie that would maximize payoffs. We also find no significant difference between willingness to tell a Pareto white lie and a selfish black lie—a lie that harms another. We find marginal evidence of more lying in China than the UK, but the overall results in the UK and China are very similar.
Current Status and Future Prospects of Perinatal Stem Cells
Authors: Torre Paz de la , Flores Ana I.
Published: December 2020

The placenta is a temporary organ that is discarded after birth and is one of the most promising sources of various cells and tissues for use in regenerative medicine and tissue engineering, both in experimental and clinical settings. The placenta has unique, intrinsic features because it plays many roles during gestation: it is formed by cells from two individuals (mother and fetus), contributes to the development and growth of an allogeneic fetus, and has two independent and interacting circulatory systems. Different stem and progenitor cell types can be isolated from the different perinatal tissues making them particularly interesting candidates for use in cell therapy and regenerative medicine. The primary source of perinatal stem cells is cord blood. Cord blood has been a well-known source of hematopoietic stem/progenitor cells since 1974. Biobanked cord blood has been used to treat different hematological and immunological disorders for over 30 years. Other perinatal tissues that are routinely discarded as medical waste contain non-hematopoietic cells with potential therapeutic value. Indeed, in advanced perinatal cell therapy trials, mesenchymal stromal cells are the most commonly used. Here, we review one by one the different perinatal tissues and the different perinatal stem cells isolated with their phenotypical characteristics and the preclinical uses of these cells in numerous pathologies. An overview of clinical applications of perinatal derived cells is also described with special emphasis on the clinical trials being carried out to treat COVID19 pneumonia. Furthermore, we describe the use of new technologies in the field of perinatal stem cells and the future directions and challenges of this fascinating and rapidly progressing field of perinatal cells and regenerative medicine.
The Developmental Transcriptome of Bagworm, Metisa plana (Lepidoptera: Psychidae) and Insights into Chitin Biosynthesis Genes
Authors: Rahmat Nur Lina , Zifruddin Anis Nadyra , Zainal Abidin Cik Mohd Rizuan , Nor Muhammad Nor-Azlan , Hassan Maizom
Published: December 2020

Bagworm, Metisa plana (Lepidoptera: Psychidae) is a ubiquitous insect pest in the oil palm plantations. M. plana infestation could reduce the oil palm productivity by 40% if it remains untreated over two consecutive years. Despite the urgency to tackle this issue, the genome and transcriptome of M. plana have not yet been fully elucidated. Here, we report a comprehensive transcriptome dataset from four different developmental stages of M. plana, comprising of egg, third instar larva, pupa and female adult. The de novo transcriptome assembly of the raw data had produced a total of 193,686 transcripts, which were then annotated against UniProt, NCBI non-redundant (NR) database, Gene Ontology, Cluster of Orthologous Group, and Kyoto Encyclopedia of Genes and Genomes databases. From this, 46,534 transcripts were annotated and mapped to 146 known metabolic or signalling KEGG pathways. The paper further identified 41 differentially expressed transcripts encoding seven genes in the chitin biosynthesis pathways, and their expressions across each developmental stage were further analysed. The genetic diversity of M. plana was profiled whereby there were 21,516 microsatellite sequences and 379,895 SNPs loci found in the transcriptome of M. plana. These datasets add valuable transcriptomic resources for further study of developmental gene expression, transcriptional regulations and functional gene activities involved in the development of M. plana. Identification of regulatory genes in the chitin biosynthesis pathway may also help in developing an RNAi-mediated pest control management by targeting certain pathways, and functional studies of the genes in M. plana.
MicroRNA 34a–AXL Axis Regulates Vasculogenic Mimicry Formation in Breast Cancer Cells
Authors: Lim Dansaem , Cho Jin Gu , Yun Eunsik , Lee Aram , Ryu Hong-Yeoul , Lee Young Joo , Yoon Sukjoon , Chang Woochul , Lee Myeong-Sok , Kwon Byung Su , Kim Jongmin
Published: December 2020

Targeting the tumor vasculature is an attractive strategy for cancer treatment. However, the tumor vasculature is heterogeneous, and the mechanisms involved in the neovascularization of tumors are highly complex. Vasculogenic mimicry (VM) refers to the formation of vessel-like structures by tumor cells, which can contribute to tumor neovascularization, and is closely related to metastasis and a poor prognosis. Here, we report a novel function of AXL receptor tyrosine kinase (AXL) in the regulation of VM formation in breast cancer cells. MDA-MB-231 cells exhibited VM formation on Matrigel cultures, whereas MCF-7 cells did not. Moreover, AXL expression was positively correlated with VM formation. Pharmacological inhibition or AXL knockdown strongly suppressed VM formation in MDA-MB-231 cells, whereas the overexpression of AXL in MCF-7 cells promoted VM formation. In addition, AXL knockdown regulated epithelial–mesenchymal transition (EMT) features, increasing cell invasion and migration in MDA-MB-231 cells. Finally, the overexpression of microRNA-34a (miR-34a), which is a well-described EMT-inhibiting miRNA and targets AXL, inhibited VM formation, migration, and invasion in MDA-MB 231 cells. These results identify a miR-34a–AXL axis that is critical for the regulation of VM formation and may serve as a therapeutic target to inhibit tumor neovascularization.
Genetic Analysis of Root-to-Shoot Signaling and Rootstock-Mediated Tolerance to Water Deficit in Tomato
Authors: Asins Maria J. , Albacete Alfonso , Martínez-Andújar Cristina , Celiktopuz Eser , Solmaz İlknur , Sarı Nebahat , Pérez-Alfocea Francisco , Dodd Ian C. , Carbonell Emilio A. , Topcu Sevilay
Published: December 2020

Developing drought-tolerant crops is an important strategy to mitigate climate change impacts. Modulating root system function provides opportunities to improve crop yield under biotic and abiotic stresses. With this aim, a commercial hybrid tomato variety was grafted on a genotyped population of 123 recombinant inbred lines (RILs) derived from Solanum pimpinellifolium, and compared with self- and non-grafted controls, under contrasting watering treatments (100% vs. 70% of crop evapotranspiration). Drought tolerance was genetically analyzed for vegetative and flowering traits, and root xylem sap phytohormone and nutrient composition. Under water deficit, around 25% of RILs conferred larger total shoot dry weight than controls. Reproductive and vegetative traits under water deficit were highly and positively correlated to the shoot water content. This association was genetically supported by linkage of quantitative trait loci (QTL) controlling these traits within four genomic regions. From a total of 83 significant QTLs, most were irrigation-regime specific. The gene contents of 8 out of 12 genomic regions containing 46 QTLs were found significantly enriched at certain GO terms and some candidate genes from diverse gene families were identified. Thus, grafting commercial varieties onto selected rootstocks derived from S. pimpinellifolium provides a viable strategy to enhance drought tolerance in tomato.
Musculoskeletal Pain and Teleworking in Times of the COVID-19: Analysis of the Impact on the Workers at Two Spanish Universities
Authors: Rodríguez-Nogueira Óscar , Leirós-Rodríguez Raquel , Benítez-Andrades José Alberto , Álvarez-Álvarez María José , Marqués-Sánchez Pilar , Pinto-Carral Arrate
Published: December 2020

The special situation brought about by the coronavirus pandemic and the confinement imposed by the Government, has given rise to numerous changes in working habits. The workers at the universities have had to start a period of teleworking that could give rise to consequences for the musculoskeletal system. The objective of this article is to analyze the impact of the confinement on the musculoskeletal health of the staff of two Spanish universities. A cross-sectional, observational study was carried out on the workers. Data was taken in April–May 2020 and included: The Standardized Kuorinka Modified Nordic Questionnaire, the Perceived Stress Scale and another one on sociodemographic data. This study comprised 472 people. The areas of pain noted during the confinement period concluded that it was less in all cases (p < 0.001). The frequency of physical activity carried out increased significantly during the period of confinement (p < 0.04), especially in women. The type of physical activity done was also seen to modify during this period (p < 0.001), with a preference for strength training and stretching exercises. In conclusion, the confinement gave rise to changes in the lifestyle and in the musculoskeletal pain of the workers at the universities. All of this must be taken into account by health institutions and those responsible for the Prevention of Occupational Risks at Spanish universities.
The Impact of Social Jetlag on Sleep Quality among Nurses: A Cross-Sectional Survey
Authors: Kang Hyeonjin , Lee Miyoung , Jang Sun Joo
Published: December 2020

Social jetlag (SJL) refers to an asynchrony between one’s chronotype and social working hours, which can be detrimental to health. The current SJL situation in shift nurses who work in dysregulation is poorly understood. Therefore, this study aimed to investigate SJL during night shifts and identify the predictors of sleep quality in rotating shift nurses. A cross-sectional study was conducted in 2018 on a sample of 132 shift-working nurses from two general hospitals in South Korea (the response rate was 88.8%). The SJL was measured with the Munich Chronotype Questionnaire, and sleep quality was measured with the Pittsburgh Sleep Quality Index and the Verran and Snyder–Halpern Sleep Scale. Data analysis was mainly based on a multiple regression, to identify SJL’s influence on nurses’ sleep quality. The average SJL during night shifts was 2 h and 3 min, and the average sleep duration during night shifts was 6 h 10 min. Multiple regression analysis revealed that SJL, day-shift fatigue, and sleep quality during night shifts affected nurses’ sleep quality. These variables accounted for 24.7% of the variance in overall sleep quality. The study concluded that overall sleep quality can increase with decreasing day-shift fatigue, decreasing SJL, and increasing sleep quality on night shifts.
Training Preferences of School Food Service Staff Vary by Role in the School Nutrition Program
Authors: Flure Leia , Pflugh Prescott Melissa , Ajie Whitney , Allison Trinity , McCaffrey Jennifer
Published: December 2020

Professional development has been identified as a critical component for school nutrition professionals (SNPs) to successfully implement school meal standards in the United States. However, training needs may vary based on different factors. This study examined (1) the topics of highest priority for SNPs; (2) preferred learning methods; (3) where and when trainings should be conducted; and (4) whether responses differ according to important factors including position type, school locale (urban vs. rural), or job experience. Participants completed surveys that included questions on demographics and preferences for learning methods and training topics (n = 492). Descriptive statistics characterized survey responses. Chi square tests assessed differences in learning method and training topic preferences by participant role, locale, and job experience; Cramer’s V assessed the strength of association for each chi square result. Qualitative responses to open-ended questions were analyzed using an inductive thematic analysis method. Nearly all training topic preferences were significantly different (p < 0.001 using Bonferroni method) when stratified by role. Significant differences were also observed for school locale and years of experience, but to a lesser degree. There was less variation in learning method preferences across staff role. Qualitative results (n = 93) identified three key themes related to training needs: role-specific trainings, innovative learning methods, and geographic access. The combination of quantitative and qualitative analysis indicate that professional development for SNPs should mostly be conducted in-person, be easily accessible, and include hands-on activities. Further, training should be tailored by job role and address situational barriers unique to the geographic area.
Triage of Patients Suspected of COVID-19 in Chronic Hemodialysis: Eosinophil Count Differentiates Low and High Suspicion of COVID-19
Authors: Vial Romain , Gully Marion , Bobot Mickael , Scarfoglière Violaine , Brunet Philippe , Bouchouareb Dammar , Duval Ariane , Zino He-oh , Faraut Julien , Jehel Océane , Berdad-Haddad Yaël , Burtey Stéphane , Jarrot Pierre-André , Lano Guillaume , Robert Thomas
Published: December 2020

Background: Daily management to shield chronic dialysis patients from SARS-CoV-2 contamination makes patient care cumbersome. There are no screening methods to date and a molecular biology platform is essential to perform RT-PCR for SARS-CoV-2; however, accessibility remains poor. Our goal was to assess whether the tools routinely used to monitor our hemodialysis patients could represent reliable and quickly accessible diagnostic indicators to improve the management of our hemodialysis patients in this pandemic environment. Methods: In this prospective observational diagnostic study, we recruited patients from La Conception hospital. Patients were eligible for inclusion if suspected of SARS-CoV-2 infection when arriving at our center for a dialysis session between March 12th and April 24th 2020. They were included if both RT-PCR result for SARS-CoV-2 and cell blood count on the day that infection was suspected were available. We calculated the area under the curve (AUC) of the receiver operating characteristic curve. Results: 37 patients were included in the final analysis, of which 16 (43.2%) were COVID-19 positive. For the day of suspected COVID-19, total leukocytes were significantly lower in the COVID-19 positive group (4.1 vs. 7.4 G/L, p = 0.0072) and were characterized by lower neutrophils (2.7 vs. 5.1 G/L, p = 0.021) and eosinophils (0.01 vs. 0.15 G/L, p = 0.0003). Eosinophil count below 0.045 G/L identified SARS-CoV-2 infection with AUC of 0.9 [95% CI 0.81—1] (p < 0.0001), sensitivity of 82%, specificity of 86%, a positive predictive value of 82%, a negative predictive value of 86% and a likelihood ratio of 6.04. Conclusions: Eosinophil count enables rapid routine screening of symptomatic chronic hemodialysis patients suspected of being COVID-19 within a range of low or high probability.
Implementation of a Standardized Post-Cesarean Delivery Order Set with Multimodal Combination Analgesia Reduces Inpatient Opioid Usage
Authors: Bornstein Eran , Husk Gregg , Lenchner Erez , Grunebaum Amos , Gadomski Therese , Zottola Cristina , Werner Sarah , Hirsch Jamie S. , Chervenak Frank A.
Published: December 2020

Background: Opioid use has emerged as a leading cause of death in the US. Given that 1 in 300 opioid-naive patients exposed to opioids after cesarean birth will become persistent users, hospitals should strive to limit exposure to these medications. We set out to evaluate whether transitioning to a standardized order set based on multimodal combination analgesic therapy decreases the exposure to opioids after cesarean delivery. Methods: Our health system’s post-cesarean pain management electronic medical record (EMR) order set was changed from standing NSAIDs (Ibuprofen 600 mg every 6 h) and additional acetaminophen and opioid medications (Oxycodone 5 mg/acetaminophen 325 mg every 3 h or Oxycodone 10 mg/acetaminophen 650 mg every 6 h for moderate and severe pain, respectively) as needed (PRN) to a multimodal combination therapy with acetaminophen (975 mg every 6 h) and NSAIDs (Ibuprofen 600 mg every 6 h) as primary analgesics and opioids PRN (Oxycodone immediate release (IR) 5 mg every 3 h for moderate to severe pain). We performed a retrospective analysis across seven hospitals comparing inpatient opioid use, administration of other analgesics, and severe pain episodes (pain score ≥ 7) between the patients who were treated before and after implementation of the multimodal order set. Chi square and Student t-test were used for statistical analysis with significance determined as p < 0.05. Results: A total of 12,898 cesarean births were included (8696 prior and 4202 after implementation). The multimodal order set was associated with marked decrease in the incidence of post cesarean opioid use (45.4% vs. 67.5%; p < 0.0001), lower average opioid dose (26.7 mg vs. 36.6 mg of oxycodone; p < 0.0001), and increased dose of acetaminophen (8422 mg vs. 4563 mg; p < 0.0001), while severe pain scores were less frequent (46.3% vs. 56.6%, p < 0.0001). Conclusions: Multimodal analgesic therapy for post-cesarean pain management reduces inpatient opioid use while improving pain control. Incorporation of a multimodal order set as a default in the EMR facilitates effective and widespread implementation on a large scale. Obstetric units should consider standardizing post-cesarean pain management orders to include routine (not PRN) multimodal combination therapy with acetaminophen and NSAIDs as primary analgesics.
Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles
Authors: Noguchi Hirofumi
Published: December 2020

The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification method using the COBE 2991 cell processor causes mechanical damage to the islets. We and other groups have shown human/porcine islet purification using large cylindrical plastic bottles. Shear stress can be minimized or eliminated using large cylindrical plastic bottles because the bottles do not have a narrow segment and no centrifugation is required during tissue loading and the collection processes of islet purification. This review describes current advances in islet purification from large mammals and humans using a COBE 2991 cell processor versus large cylindrical plastic bottles.
Alteration within the Hippocampal Volume in Patients with LHON Disease—7 Tesla MRI Study
Authors: Grochowski Cezary , Jonak Kamil , Maciejewski Marcin , Stępniewski Andrzej , Rahnama-Hezavah Mansur
Published: December 2020

Purpose: The aim of this study was to assess the volumetry of the hippocampus in the Leber’s hereditary optic neuropathy (LHON) of blind patients. Methods: A total of 25 patients with LHON were randomly included into the study from the national health database. A total of 15 patients were selected according to the inclusion criteria. The submillimeter segmentation of the hippocampus was based on three-dimensional spoiled gradient recalled acquisition in steady state (3D-SPGR) BRAVO 7T magnetic resonance imaging (MRI) protocol. Results: Statistical analysis revealed that compared to healthy controls (HC), LHON subjects had multiple significant differences only in the right hippocampus, including a significantly higher volume of hippocampal tail (p = 0.009), subiculum body (p = 0.018), CA1 body (p = 0.002), hippocampal fissure (p = 0.046), molecular layer hippocampus (HP) body (p = 0.014), CA3 body (p = 0.006), Granule Cell (GC) and Molecular Layer (ML) of the Dentate Gyrus (DG)–GC ML DG body (p = 0.003), CA4 body (p = 0.001), whole hippocampal body (p = 0.018), and the whole hippocampus volume (p = 0.023). Discussion: The ultra-high-field magnetic resonance imaging allowed hippocampus quality visualization and analysis, serving as a powerful in vivo diagnostic tool in the diagnostic process and LHON disease course assessment. The study confirmed previous reports regarding volumetry of hippocampus in blind individuals.
Prevention of Stricture after Endoscopic Submucosal Dissection for Superficial Esophageal Cancer: A Review of the Literature
Authors: Hikichi Takuto , Nakamura Jun , Takasumi Mika , Hashimoto Minami , Kato Tsunetaka , Kobashi Ryoichiro , Takagi Tadayuki , Suzuki Rei , Sugimoto Mitsuru , Sato Yuki , Irie Hiroki , Okubo Yoshinori , Kobayakawa Masao , Ohira Hiromasa
Published: December 2020

Endoscopic resection has been the standard treatment for intramucosal esophageal cancers (ECs) because of the low risk of lymph node metastases in the lesions. In recent years, endoscopic submucosal dissection (ESD), which can resect large ECs, has been performed. However, the risk of esophageal stricture after ESD is high when the mucosal defect caused by the treatment exceeds 3/4 of the circumference of the lumen. Despite the subsequent high risk of luminal stricture, ESD has been performed even in cases of circumferential EC. In such cases, it is necessary to take measures to prevent stricture. Therefore, in this review, we aimed to clarify the current status of stricture prevention methods after esophageal ESD based on previous literature. Although various prophylactic methods have been reported to have stricture-preventing effects, steroid injection therapy and oral steroid administration are mainstream. However, in cases of circumferential EC, both steroid injection therapy and oral steroid administration cannot effectively prevent luminal stricture. To solve this issue, clinical applications, such as tissue shielding methods with polyglycolic acid sheet, autologous oral mucosal epithelial sheet transplantation, and stent placement, have been developed. However, effective prophylaxis of post-ESD mucosal defects of the esophagus is still unclear. Therefore, further studies in this research field are needed.
Validation of a Coupled Simulation for Machine Tool Dynamics Using a Linear Drive Actuator
Authors: Wiesauer Michael , Habersohn Christoph , Bleicher Friedrich
Published: December 2020

In order to ensure high productivity capabilities of machine tools at a low cost but at increased geometric accuracy, modeling of their static and dynamic behavior is a crucial task in structure optimization. The drive control and the frictional forces acting in feed axes significantly determine the machine’s response in the frequency domain. The aim of this study was the accurate modeling and the experimental investigation of dynamic damping effects using a machine tool test rig with three-axis kinematics. For this purpose, an order-reduced finite element model of the mechanical structure was coupled with models of the drive control and of the non-linear friction behavior. In order to validate the individual models, a new actuator system based on a tubular linear drive was used for frequency response measurements during uniaxial carriage movements. A comparison of the dynamic measurements with the simulation results revealed a good match of amplitudes in the frequency domain by considering dynamic damping. Accordingly, the overall dynamic behavior of machine tool structures can be predicted and thus optimized by a coupled simulation at higher level of detail and by considering the damping effects of friction. Dynamic testing with the newly designed actuator is a prerequisite for model validation and control drive parameterization.
Gene Editing Targeting the DUX4 Polyadenylation Signal: A Therapy for FSHD?
Authors: Joubert Romains , Mariot Virginie , Charpentier Marine , Concordet Jean Paul , Dumonceaux Julie
Published: December 2020

Facioscapulohumeral dystrophy (FSHD, OMIM: 158900, 158901) is the most common dystrophy in adults and so far, there is no treatment. Different loci of the disease have been characterized and they all lead to the aberrant expression of the DUX4 protein, which impairs the function of the muscle, ultimately leading to cell death. Here, we used gene editing to try to permanently shut down DUX4 expression by targeting its poly(A) sequence. We used transcription activator-like effector nucleases (TALEN) and CRISPR-Cas9 nucleases in vitro on FSHD myoblasts. More than 150 TOPO clones were sequenced and only indels were observed in 4%. Importantly, in 2 of them, the DUX4 poly(A) signal was eliminated at the genomic level but DUX4 mRNA was still produced thanks to the use of a non-canonical upstream poly(A) signal sequence. These experiments show that targeting DUX4 PAS at the genomic level might not be an appropriate gene editing strategy for FSHD therapy.
Smallholder Commercialization and Urban-Rural Linkages: Effect of Interest-Free Agriculture Credit on Market Participation of Rice Growers in Pakistan
Authors: Sher Ali , Mazhar Saman , Azadi Hossein , Lin Guanghua
Published: December 2020

This study estimates the effect of interest-free agriculture credit on the market participation and urban-rural linkages of rice growers in Pakistan. A survey was conducted to collect primary data using purposive and simple random sampling techniques from Punjab, Pakistan. This study applied the Instrument Variable (IV) approach and Ordinary Least Square (OLS) to evaluate the impact of interest-free credit on market participation and income. The results show a mixed influence of interest-free credit on rice growers’ market participation and urban-rural linkages. In general, the effect is negative when farmers obtained credit for six months. However, it shows a positive impact when farmers’ received credit for the next consecutive crop. Our findings suggest that the provision of interest-free credit for one year served a better purpose as it significantly attempted to alleviate budget constraints and endorsed farmers to increase land size under rice cultivation and improve productivity, market participation, and urban-rural linkages. The study provides three valid instruments and, therefore, a superior estimate of effect is achieved which can be leveraged to better support coherent agri-food policymaking.
Impact of Sustainable Land Management Practices on Soil Properties: Example of Organic and Integrated Agricultural Management
Authors: Mihelič Rok , Pečnik Jure , Glavan Matjaž , Pintar Marina
Published: December 2020

Maintaining good soil quality is crucial for the sustainability of agriculture. This study aimed to evaluate the effectiveness of the visual soil assessment (VSA) method by testing it on two soil types and two agricultural management practices (AMP) (organic and integrated) that are considered to protect soil quality. We selected two farms with plots on two river terraces with different soil properties. The test was based on the modified method Annual Crops Visual Quality Assessment developed by the Food and Agriculture Organization of the United Nations and supported by a standardized soil physical and chemical analysis. This study showed that the assessed score is highly dependent on the type of farming practice and how soils are managed. The soil type also plays an important role. The results for Calcaric Fluvisol showed that the effects of selected agricultural management practices on the visual assessment of soil quality could be almost undetectable. The time of assessment also plays a significant role in VSA scoring. Different crops and agricultural activities with significant impacts on the soil occur throughout the year (especially in vegetable production). It was observed that a higher score for the soil cover indicator had a beneficial effect on the total VSA rating.
Language Experience Affects Comprehension of Spanish Passive Clauses: A Study of Heritage Speakers and Second Language Learners
Authors: Sánchez Walker Noelia , Montrul Silvina
Published: December 2020

Heritage language (HL) learners of Spanish have shown better command with early acquired aspects of grammar than second language (L2) learners, mainly in oral tasks. This study investigates whether this advantage persists with passive clauses, structures acquired early but mastered during the school-age years, with literacy. We examined adjectival passives (La comida estaba servida, “Dinner was served”) with the copula estar in the imperfect, which refer to a description of a state or a final result; and verbal passives with the copula ser in the imperfect (La comida era servida. “Dinner was being served”), which refer to an ongoing or habitual action in the past. A grammaticality judgment task (GJT) testing knowledge of the copulas in different simple sentences and a picture-matching task (PMT) testing the comprehension of the two passive clauses revealed that HL learners’ knowledge of the copulas resembles that of literate monolingually raised native speakers more than that of L2 learners. HL learners are able to integrate their knowledge of the copulas to comprehend syntactically complex clauses, especially in the aural modality.
The Structure and Magnetic Properties of Rapidly Quenched Fe72Ni8Nb4Si2B14 Alloy
Authors: Hawelek Lukasz , Warski Tymon , Wlodarczyk Patryk , Polak Marcin , Zackiewicz Przemyslaw , Maziarz Wojciech , Wojcik Anna , Steczkowska-Kempka Magdalena , Kolano-Burian Aleksandra
Published: December 2020

The complex structural and magnetic studies of the annealed rapidly quenched Cu-free Fe72Ni8Nb4Si2B14 alloy (metallic ribbons form) are reported here. Based on the calorimetric results, the conventional heat treatment process (with heating rate 10 °C/min and subsequent isothermal annealing for 20 min) for wound toroidal cores has been optimized to obtain the least lossy magnetic properties (for the minimum value of coercivity and magnetic core losses at 50 Hz). For optimal conditions, the complex permeability in the 104–108 Hz frequency range together with core power losses obtained from magnetic induction dependence up to the frequency of 400 kHz was successfully measured. The average and local crystal structure was investigated by the use of the X-ray diffraction method and the transmission electron microscopy observations and proved its fully glassy state. Additionally, for the three temperature values, i.e., 310, 340 and 370 °C, the glass relaxation process study in the function of annealing time was carried out to obtain a deeper insight into the soft magnetic properties: magnetic permeability and cut-off frequency. For this type of Cu-free soft magnetic materials, the control of glass relaxation process (time and temperature) is extremely important to obtain proper magnetic properties.
Laser-Induced Deposition of Plasmonic Ag and Pt Nanoparticles, and Periodic Arrays
Authors: Mamonova Daria V. , Vasileva Anna A. , Petrov Yuri V. , Danilov Denis V. , Kolesnikov Ilya E. , Kalinichev Alexey A. , Bachmann Julien , Manshina Alina A.
Published: December 2020

Surfaces functionalized with metal nanoparticles (NPs) are of great interest due to their wide potential applications in sensing, biomedicine, nanophotonics, etc. However, the precisely controllable decoration with plasmonic nanoparticles requires sophisticated techniques that are often multistep and complex. Here, we present a laser-induced deposition (LID) approach allowing for single-step surface decoration with NPs of controllable composition, morphology, and spatial distribution. The formation of Ag, Pt, and mixed Ag-Pt nanoparticles on a substrate surface was successfully demonstrated as a result of the LID process from commercially available precursors. The deposited nanoparticles were characterized with SEM, TEM, EDX, X-ray diffraction, and UV-VIS absorption spectroscopy, which confirmed the formation of crystalline nanoparticles of Pt (3–5 nm) and Ag (ca. 100 nm) with plasmonic properties. The advantageous features of the LID process allow us to demonstrate the spatially selective deposition of plasmonic NPs in a laser interference pattern, and thereby, the formation of periodic arrays of Ag NPs forming diffraction grating
Post-Processing Time Dependence of Shrinkage and Mechanical Properties of Injection-Molded Polypropylene
Authors: Kościuszko Artur , Marciniak Dawid , Sykutera Dariusz
Published: December 2020

Dimensions of the injection-molded semi-crystalline materials (polymeric products) decrease with the time that elapses from their formation. The post-molding shrinkage is an effect of secondary crystallization; the increase in the degree of polymer crystallinity leads to an increase in stiffness and decrease in impact strength of the polymer material. The aim of this study was to assess the changes in the values of post-molding shrinkage of polypropylene produced by injection molding at two different temperatures of the mold (20 °C and 80 °C), and conditioned for 504 h at 23 °C. Subsequently, the samples were annealed for 24 h at 140 °C in order to conduct their accelerated aging. The results of shrinkage tests were related to the changes of mechanical properties that accompany the secondary crystallization. The degree of crystallinity of the conditioned samples was determined by means of density measurements and differential scanning calorimetry. It was found that the changes in the length of the moldings that took place after removal from the injection mold were accompanied by an increase of 20% in the modulus of elasticity, regardless of the conditions under which the samples were made. The differences in the shrinkage and mechanical properties of the samples resulting from mold temperature, as determined by tensile test, were removed by annealing. However, the samples made at two different injection mold temperature values still significantly differed in impact strength, the values of which were clearly higher for the annealed samples compared to the results determined for the samples immediately after the injection molding.
Accuracy of Full-Guided and Half-Guided Surgical Templates in Anterior Immediate and Delayed Implantation: A Retrospective Study
Authors: Chen Yuan , Zhang Xiaoqing , Wang Maoxia , Jiang Qingling , Mo Anchun
Published: December 2020

Computer-aided implantology has developed rapidly in recent years, this study aimed to compare the accuracy of guided-surgery between anterior immediate and delayed implantation, and simultaneously assess the effect of full-guided and half-guided templates on accuracy values. Seventy-six implants were inserted in 63 patients using full-guided or half-guided template in the anterior zone. Postoperative cone beam computed tomography (CBCT) was matched with preoperative planning to evaluate the deviation between actual and planned implants. No statistical difference was found in any deviation between immediate and delayed implantation (p > 0.05). In anterior immediate implantation, the global coronal, apical, depth and angular deviations of full-guided templates were all significantly lower than those of half-guided templates (0.66 ± 0.26 vs. 1.10 ± 0.76 mm, 0.96 ± 0.41 vs. 1.43 ± 0.70 mm, 0.46 ± 0.24 mm vs. 0.93 ± 0.79 mm and 1.69° ± 0.94° vs. 2.57° ± 1.57°). While in delayed implantation, full-guided templates only perform better with statistical significance on global apical and depth deviation (1.01 ± 0.42 vs. 1.51 ± 0.55 mm and 0.32 ± 0.26 vs. 0.71 ± 0.47 mm). After excluding the influence of depth deviation, the coronal and apical deviations between the two systems in immediate implantation and the apical deviations in delayed implantation had no statistical difference. Within the limit of this study, the results suggested the accuracy of guided-surgeries for anterior immediate and delayed implantations was comparable, and full-guided template was more accurate for immediate and delayed implantation.
Double-Inverse-Opal-Structured Particle Assembly as a Novel Immobilized Photocatalytic Material
Authors: Namigata Hikaru , Watanabe Kanako , Okubo Saya , Hasegawa Masashi , Suga Keishi , Nagao Daisuke
Published: December 2020

Immobilization of photocatalysts on supports is an important method of adding highly active photocatalysts to a continuous flowing system without the need for photocatalyst recovery. However, direct immobilization prevents exposure to all photocatalytically active surfaces. Therefore, to immobilize particulate photocatalysts, while exposing the photocatalytic surface to organic pollutant water in a continuous flowing system, in this study, we employed double-inverse-opal (DIO) with periodically arranged, interconnected macropores, each containing a single photocatalytic particle. Increasing the macropore size successfully enhanced the decomposition rate of organic dye due to the high diffusion rate of dye molecules in the macropores of thin DIOs. However, an excessive increase in macropore size lowered the decomposition rate of dye molecules because an increase in DIO thickness caused the attenuation of light used to excite the photocatalytic particles. This study presents novel, immobilized photocatalytic DIO-structured particles that can be employed in continuous flowing reaction systems by tuning the photocatalytic particle size, macropore size, and DIO thickness.
Microleakage Analysis of Different Bulk-Filling Techniques for Class II Restorations: µ-CT, SEM and EDS Evaluations
Authors: Tosco Vincenzo , Vitiello Flavia , Furlani Michele , Gatto Maria Laura , Monterubbianesi Riccardo , Giuliani Alessandra , Orsini Giovanna , Putignano Angelo
Published: December 2020

This study aimed to compare two different bulk-filling techniques, evaluating the internal and external adaptation of class II resin-composite restorations, by analysing the gap formation using microcomputed tomography (µ-CT) and scanning electronic microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS). Two standardized mesio/disto-occlusal (MO/DO) cavities were prepared in eight extracted human third molars that were divided, according to the filling technique used, in the following two groups (n = 4): BG (Bulk&Go group) and BT (Bulk Traditional group). After universal bonding application, followed by the light curing, all teeth were restored using a bulk-fill composite. Specimens were scanned with µ-CT to evaluate 3D interfacial gaps. Acquired µ-CT data were analysed to quantify the gap formation. Complementary information to the µ-CT analysis were obtained by SEM. Thereafter, the chemical composition of tooth–restoration interface was analysed using EDS. The µ-CT analysis revealed gaps formation at the tooth–restoration interface for both the BG and BT groups, while within the restoration, only in the BT group there was evidence of microleakage formation. The scanning electron micrographs of both groups showed that the external marginal integrity of the restoration was preserved, while EDS showed the three different structures (tooth surface, adhesive layer and resin composite) of the tooth–restoration interface, highlighting the absence of gap formation. In both BG and BT, the two filling techniques did not show significant differences regarding the internal and external marginal adaptation of the restoration. To achieve a successful restoration, the clinician could be advised to restore a class II cavity using a single increment bulk-filling technique (BG), thus treating it as a class I cavity.
The Remarkable Antioxidant and Anti-Inflammatory Potential of the Extracts of the Brown Alga Cystoseira amentacea var. stricta
Authors: De La Fuente Gina , Fontana Marco , Asnaghi Valentina , Chiantore Mariachiara , Mirata Serena , Salis Annalisa , Damonte Gianluca , Scarfì Sonia
Published: December 2020

Inflammation and oxidative stress are part of the complex biological responses of body tissues to harmful stimuli. In recent years, due to the increased understanding that oxidative stress is implicated in several diseases, pharmaceutical industries have invested in the research and development of new antioxidant compounds, especially from marine environment sources. Marine seaweeds have shown the presence of many bioactive secondary metabolites, with great potentialities from both the nutraceutical and the biomedical point of view. In this study, 50%-ethanolic and DMSO extracts from the species C. amentacea var. stricta were obtained for the first time from seaweeds collected in the Ligurian Sea (north-western Mediterranean). The bioactive properties of these extracts were then investigated, in terms of quantification of specific antioxidant activities by relevant ROS scavenging spectrophotometric tests, and of anti-inflammatory properties in LPS-stimulated macrophages by evaluation of inhibition of inflammatory cytokines and mediators. The data obtained in this study demonstrate a strong anti-inflammatory effect of both C. amentacea extracts (DMSO and ethanolic). The extracts showed a very low grade of toxicity on RAW 264.7 macrophages and L929 fibroblasts and a plethora of antioxidant and anti-inflammatory effects that were for the first time thoroughly investigated. The two extracts were able to scavenge OH and NO radicals (OH EC50 between 392 and 454 μg/mL; NO EC50 between 546 and 1293 μg/mL), to partially rescue H2O2-induced RAW 264.7 macrophages cell death, to abate intracellular ROS production in H2O2-stimulated macrophages and fibroblasts and to strongly inhibit LPS-induced inflammatory mediators, such as NO production and IL-1α, IL-6, cyclooxygenase-2 and inducible NO synthase gene expression in RAW 264.7 macrophages. These results pave the way, for the future use of C. amentacea metabolites, as an example, as antioxidant food additives in antiaging formulations as well as in cosmetic lenitive lotions for inflamed and/or damaged skin.
Insights into the Membranolytic Activity of Antimalarial Drug-Cell Penetrating Peptide Conjugates
Authors: Aguiar Luísa , Pinheiro Marina , Neves Ana Rute , Vale Nuno , Defaus Sira , Andreu David , Reis Salette , Gomes Paula
Published: December 2020

Conjugation of TP10, a cell-penetrating peptide with intrinsic antimalarial activity, to the well-known antimalarial drugs chloroquine and primaquine has been previously shown to enhance the peptide’s action against, respectively, blood- and liver-stage malaria parasites. Yet, this was achieved at the cost of a significant increase in haemolytic activity, as fluorescence microscopy and flow cytometry studies showed the conjugates to be more haemolytic for non-infected than for Plasmodium-infected red blood cells. To gain further insight into how these conjugates distinctively bind, and likely disrupt, membranes of both Plasmodium-infected and non-infected erythrocytes, we used dynamic light scattering and surface plasmon resonance to study the interactions of two representative conjugates and their parent compounds with lipid model membranes. Results obtained are herein reported and confirm that a strong membrane-disruptive character underlies the haemolytic properties of these conjugates, thus hampering their ability to exert selective antimalarial action.
Recent Progress in One- and Two-Dimensional Nanomaterial-Based Electro-Responsive Membranes: Versatile and Smart Applications from Fouling Mitigation to Tuning Mass Transport
Authors: Alayande Abayomi Babatunde , Goh Kunli , Son Moon , Kim Chang-Min , Chae Kyu-Jung , Kang Yesol , Jang Jaewon , Kim In S. , Yang Euntae
Published: December 2020

Membrane technologies are playing an ever-important role in the field of water treatment since water reuse and desalination were put in place as alternative water resources to alleviate the global water crisis. Recently, membranes are becoming more versatile and powerful with upgraded electroconductive capabilities, owing to the development of novel materials (e.g., carbon nanotubes and graphene) with dual properties for assembling into membranes and exerting electrochemical activities. Novel nanomaterial-based electrically responsive membranes have been employed with promising results for mitigating membrane fouling, enhancing membrane separation performance and self-cleaning ability, controlling membrane wettability, etc. In this article, recent progress in novel-nanomaterial-based electrically responsive membranes for application in the field of water purification are provided. Thereafter, several critical drawbacks and future outlooks are discussed.