bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of an Image-Derived Input Function for Kinetic Modeling of Nicotinic Acetylcholine Receptor-Binding PET Ligands in Mice
Authors: Zammit Matthew , Kao Chien-Min , Zhang Hannah J. , Tsai Hsiu-Ming , Holderman Nathanial , Mitchell Samuel , Tanios Eve , Bhuiyan Mohammed , Freifelder Richard , Kucharski Anna , Green William N. , Mukherjee Jogeshwar , Chen Chin-Tu
Published: October 2023

Positron emission tomography (PET) radioligands that bind with high-affinity to α4β2-type nicotinic receptors (α4β2Rs) allow for in vivo investigations of the mechanisms underlying nicotine addiction and smoking cessation. Here, we investigate the use of an image-derived arterial input function and the cerebellum for kinetic analysis of radioligand binding in mice. Two radioligands were explored: 2-[18F]FA85380 (2-FA), displaying similar pKa and binding affinity to the smoking cessation drug varenicline (Chantix), and [18F]Nifene, displaying similar pKa and binding affinity to nicotine. Time–activity curves of the left ventricle of the heart displayed similar distribution across wild type mice, mice lacking the β2-subunit for ligand binding, and acute nicotine-treated mice, whereas reference tissue binding displayed high variation between groups. Binding potential estimated from a two-tissue compartment model fit of the data with the image-derived input function were higher than estimates from reference tissue-based estimations. Rate constants of radioligand dissociation were very slow for 2-FA and very fast for Nifene. We conclude that using an image-derived input function for kinetic modeling of nicotinic PET ligands provides suitable results compared to reference tissue-based methods and that the chemical properties of 2-FA and Nifene are suitable to study receptor response to nicotine addiction and smoking cessation therapies.
Src Tyrosine Kinase Inhibitory and Antioxidant Activity of Black Chokeberry and Bilberry Fruit Extracts Rich in Chlorogenic Acid
Authors: Vladimir-Knežević Sanda , Bival Štefan Maja , Blažeković Biljana , Jelić Dubravko , Petković Tea , Mandić Marta , Šprajc Ekaterina , Lovković Sandy
Published: October 2023

Edible berries such as the fruits of black chokeberry (Aronia melanocarpa (Michx.) Elliott) and bilberry (Vaccinium myrtillus L.) are considered to be rich in phenolic compounds, which are nowadays attracting great interest due to their promising health benefits. The main objective of our study was to investigate, for the first time, their inhibitory properties on Src tyrosine kinase activity, as this enzyme plays an important role in multiple cellular processes and is activated in both cancer and inflammatory cells. In hydroethanolic fruit extracts, 5.0–5.9% of total polyphenols were determined spectrophotometrically, including high amounts of hydroxycinnamic acid derivatives. HPLC analysis revealed that the black chokeberry and bilberry extracts contained 2.05 mg/g and 2.54 mg/g of chlorogenic acid, respectively. Using a time-resolved fluorescence resonance energy transfer (TR-FRET) assay, the extracts studied were found to have comparable inhibitory effects on Src tyrosine kinase, with IC50 values of 366 µg/mL and 369 µg/mL, respectively. The results also indicated that chlorogenic acid contributes significantly to the observed effect. In addition, both fruit extracts exhibited antioxidant activity by scavenging DPPH and NO radicals with SC50 values of 153–352 µg/mL. Our study suggested that black chokeberry and bilberry fruits may be beneficial in cancer and other inflammation-related diseases.
A Novel Quinazoline Derivative Prevents and Treats Arsenic-Induced Liver Injury by Regulating the Expression of RecQ Family Helicase
Authors: Yang Heping , Mo Min , Yang Langlang , Yu Jia , Li Jiao , Cheng Sha , Sun Baofei , Xu Bixue , Zhang Aihua , Luo Heng
Published: October 2023

Arsenic is a carcinogenic metalloid toxicant widely found in the natural environment. Acute or prolonged exposure to arsenic causes a series of damages to the organs, mainly the liver, such as hepatomegaly, liver fibrosis, cirrhosis, and even hepatocellular carcinoma. Therefore, it is imperative to seek drugs to prevent arsenic-induced liver injury. Quinazolines are a class of nitrogen heterocyclic compounds with biological and pharmacological effects in vivo and in vitro. This study was designed to investigate the ameliorating effects of quinazoline derivatives on arsenic-induced liver injury and its molecular mechanism. We investigated the mechanism of the quinazoline derivative KZL-047 in preventing and ameliorating arsenic-induced liver injury in vitro by cell cycle and apoptosis. We performed real-time fluorescence quantitative polymerase chain reaction (qPCR) and Western blotting combined with molecular docking. In vivo, the experiments were performed to investigate the mechanism of KZL-047 in preventing and ameliorating arsenic-induced liver injury using arsenic-infected mice. Physiological and biochemical indices of liver function in mouse serum were measured, histopathological changes in liver tissue were observed, and immunohistochemical staining was used to detect changes in the expression of RecQ-family helicases in mouse liver tissue. The results of in vitro experiments showed that sodium arsenite (SA) inhibited the proliferation of L-02 cells, induced apoptosis, blocked the cell cycle at the G1 phase, and decreased the expression of RecQ family helicase; after KZL-047 treatment in arsenic-induced L-02 cells, the expression of RecQ family helicase was upregulated, and the apoptosis rate was slowed, leading to the restoration of the cell viability level. KZL-047 inhibited arsenic-induced oxidative stress, alleviated oxidative damage and lipid peroxidation in vivo, and ameliorated arsenic toxicity-induced liver injury. KZL-047 restored the expression of RecQ family helicase proteins, which is consistent with the results of in vitro studies. In summary, KZL-047 can be considered a potential candidate for the treatment of arsenic-induced liver injury.
Dimethyl Fumarate Attenuates Lymphocyte Infiltration and Reduces Infarct Size in Experimental Stroke
Authors: Schuhmann Michael K. , Langhauser Friederike , Zimmermann Lena , Bellut Maximilian , Kleinschnitz Christoph , Fluri Felix
Published: October 2023

Ischemic stroke is associated with exacerbated tissue damage caused by the activation of immune cells and the initiation of other inflammatory processes. Dimethyl fumarate (DMF) is known to modulate the immune response, activate antioxidative pathways, and improve the blood–brain barrier (BBB) after stroke. However, the specific impact of DMF on immune cells after cerebral ischemia remains unclear. In our study, male mice underwent transient middle cerebral artery occlusion (tMCAO) for 30 min and received oral DMF (15 mg/kg) or a vehicle immediately after tMCAO, followed by twice-daily administrations for 7 days. Infarct volume was assessed on T2-weighted magnetic resonance images on days 1 and 7 after tMCAO. Brain-infiltrating immune cells (lymphocytes, monocytes) and microglia were quantified using fluorescence-activated cell sorting. DMF treatment significantly reduced infarct volumes and brain edema. On day 1 after tMCAO, DMF-treated mice showed reduced lymphocyte infiltration compared to controls, which was not observed on day 7. Monocyte and microglial cell counts did not differ between groups on either day. In the acute phase of stroke, DMF administration attenuated lymphocyte infiltration, probably due to its stabilizing effect on the BBB. This highlights the potential of DMF as a therapeutic candidate for mitigating immune cell-driven damage in stroke.
Insertion of Transposable Elements in AVR-Pib of Magnaporthe oryzae Leading to LOSS of the Avirulent Function
Authors: Li Jinbin , Lu Lin , Li Chengyun , Wang Qun , Shi Zhufeng
Published: October 2023

Rice blast is a very serious disease caused by Magnaporthe oryzae, which threatens rice production and food supply throughout the world. The avirulence (AVR) genes of rice blast are perceived by the corresponding rice blast resistance (R) genes and prompt specific resistance. A mutation in AVR is a major force for new virulence. Exploring mutations in AVR among M. oryzae isolates from rice production fields could aid assessment of the efficacy and durability of R genes. We studied the probable molecular-evolutionary patterns of AVR-Pib alleles by assaying their DNA-sequence diversification and examining their avirulence to the corresponding Pib resistance gene under natural conditions in the extremely genetically diverse of rice resources of Yunnan, China. PCRs detected results from M. oryzae genomic DNA and revealed that 162 out of 366 isolates collected from Yunnan Province contained AVR-Pib alleles. Among them, 36.1–73.3% isolates from six different rice production areas of Yunnan contained AVR-Pib alleles. Furthermore, 36 (28.6%) out of 126 isolates had a transposable element (TE) insertion in AVR-Pib, which resulted in altered virulence. The TE insertion was identified in isolates from rice rather than from Musa nana Lour. Twelve AVR-Pib haplotypes encoding three novel AVR-Pib variants were identified among the remaining 90 isolates. AVR-Pib alleles evolved to virulent forms from avirulent forms by base substitution and TE insertion of Pot2 and Pot3 in the 5′ untranslated region of AVR-Pib. These findings support the hypothesis that functional AVR-Pib possesses varied sequence structures and can escape surveillance by hosts via multiple variation manners.
Practical Aspects of Correlation Analysis of Compressive Strength from Destructive and Non-Destructive Methods in Different Directions
Authors: Badarloo Baitollah , Lehner Petr
Published: October 2023

The research presented here demonstrates the practical aspects of the numerical correlation of the results of the compressive strength test. The destructive test (DT) in a hydraulic press and the non-destructive test (NDT) using a Schmidt hammer in several process variations were evaluated. The aim was to evaluate the real differences between the tool supplier’s curve and testing. Therefore, 150 concrete cube specimens with an edge length of 150 mm were produced using a mixture of three types of concrete classes: C30, C35, and C40. The test was carried out 7 and 28 days of age of the concrete. The Schmidt hammer test was carried out in horizontal (θ = 0) and vertical (θ = 90) directions and using a series of 10 measurements. Furthermore, the tests were performed in two sets: first, the sample was placed on the ground, and second, under a hydraulic jack with a load of 50% of the maximum bearing capacity of specific concrete. Then, regression analysis was performed on the data sets to establish linear mathematical relationships between compressive strength and number of bounces. The results showed that the correlation between the DT and NDT tests has a high value for each group, but the correlation equations are different and must be taken into account.
Combined Coastal Sea Level Estimation Considering Astronomical Tide and Storm Surge Effects: Model Development and Its Application in Thermaikos Gulf, Greece
Authors: Papadopoulos Nestoras , Gikas Vassilis
Published: October 2023

Tide gauge recordings furnish the longest and almost the most continuous data source of sea level monitoring. Traditionally, they are collected using tide gauge instrumentation fixed at seaport locations to provide a time series of sea level estimates relative to a local geodetic benchmark. Sea level tidal observables are distinguished in the astronomical tide component originating from the attraction of the Earth–Moon–Sun gravitational system, and the storm surges ought to have meteorological effects due to wind and atmospheric air pressure variation. This study provides a comprehensive methodological approach and software to compute sea level considering astronomical tides enhanced by storm surge effects. The model is realized and assessed using a long-standing set of 21 consecutive years of tidal and meteorological measurements originating from Thermaikos Gulf, Greece. Analyses show model verification and conclusions about the tidal behavior of the test area, suggesting a satisfactory agreement (86% Willmott Skill factor, 9 cm standard deviation) between predicted and observed sea level estimates, accounting for amplitude and the time shift of skew surges.
A Paleo-Changjiang Delta Complex on the East China Sea Shelf Formed Some 30 ka Ago (at the MIS 3 Stage)
Authors: Dai Chen , Gao Shu , Zhang Yongzhan , Xia Fei , Wang Dandan
Published: October 2023

The distribution patterns of the paleo-Changjiang River deltas formed during the MIS 3 period before the last glacial maximum (LGM) contain important information about the deltaic deposits in response to climate and sea-level changes. However, so far, they are still poorly understood. Here, we reconstruct the stratigraphic and chronological framework of the deltaic system based on the analysis of 1835 km of high-resolution seismic profiles obtained from the northern East China Sea, together with a comparison with the research results from four existing boreholes and three groups of published seismic profiles from the study area. Within the strata from MIS 5 to MIS 1 that are preserved on the northern shelf of the East China Sea, we discovered a large-scale paleodeltaic complex formed during the MIS 3 period. During MIS 3, sea level dropped slowly; the paleodelta formed was large in scale and was distributed between water depths of 40 and 150 m. It is now directly exposed at the seabed at a depth of more than 100 m. The paleodelta had extended from the northwest to the southeast, with maximum thickness of the deposits around 55 m. The extensions in the east–west and south–north directions both exceeded 300 km, with a total area around 1.35 × 105 km2. Since the late Pleistocene, the tectonic structure beneath the East China Sea has been relatively stable. As such, sea-level fluctuations, sediment supply, and original topography were the main controlling factors for the development of the delta.
Influence of Blade Flexibility on the Dynamic Behaviors of Monopile-Supported Offshore Wind Turbines
Authors: Lai Yongqing , Li Wei , He Ben , Xiong Gen , Xi Renqiang , Wang Piguang
Published: October 2023

At present, monopile-supported offshore wind turbines (MOWTs) are widely used in offshore wind farms. The influence of blade flexibility on the dynamic behaviors of MOWTs excited by waves and earthquakes was investigated in this study. Numerical analysis models were established for 5 MW and 10 MW MOWTs, incorporating flexible and rigid blade configurations. The modes and natural frequencies of the full system were compared between these two numerical models, and their dynamic responses were evaluated under wave-only and earthquake-only excitations. It was revealed that the influence of blade flexibility on the first- and second-order modes of the system can be neglected. The dynamic response of these MOWTs under wave excitation can be predicted by the rigid blade model, where the maximum relative difference is less than 5%. However, higher-order modes of the system are significantly affected by the blade flexibility. Under high-frequency excitations, these higher-order modes of the system are remarkably stimulated. Additionally, a large relative difference, exceeding 50%, is detected when the rigid blade model is used to predict the seismic response of the two MOWTs. Consequently, the blade flexibility should be adequately modeled when predicting the dynamic response of OWTs.
Five New Species of Aureoboletus and Chalciporus (Boletaceae, Boletales) and Their Ethnomycological Aspects
Authors: Ayala-Vásquez Olivia , Martínez-Reyes Magdalena , Pérez-Moreno Jesús , Martínez-González César Ramiro , Pinzón Juan Pablo , de la Fuente Javier Isaac , Castro-Rivera Rigoberto , García-Jiménez Jesús , Balbuena-Carrasco Soledad , Ramírez-Carbajal Eliseete , Yu Fuqiang
Published: October 2023

Among Boletales, the family Boletaceae has the highest diversity worldwide. Additionally, this fungal group has great ecological relevance because it not only includes mainly ectomycorrhizal but also saprotrophic species. Furthermore, some species are used as food and have sociocultural and economic importance worldwide. In Mexico, the Boletaceae family boasts a substantial number of species, yet our understanding of these species remains far from comprehensive. In this work, by using macro- and micromorphological and phylogenetic analyses of DNA sequences from multi-gene analyses based on ITS, nrLSU, rpb1, rpb2, and tef1, we report five new species belonging to the genera Aureoboletus and Chalciporus: A. ayuukii and A. elvirae from a Quercus scytophylla forest, A. readii from a mixed forest, C. perezsilvae from cloud forest, and C. piedracanteadensis from both a mixed coniferous forest and a Quercus-Pinus forest. In Mexico, four species of Aureoboletus are used as a food source, and in this work, we add another one, A. readii, which is traditionally consumed by members of the Tlahuica-Pjiekakjoo culture, who are located in the central part of the country. This work contributes to our knowledge of two genera of Boletaceae in a geographical area that is scarcely studied, and thus, our understanding of its biocultural relevance is enriched.
Do Changes in Risk Perception Predict Systemic Banking Crises?
Authors: Roy Saktinil
Published: October 2023

This paper examines if incorporating changes in financial market risk perception improves the predictive power of an early-warning system for systemic banking crises. In explaining systemic banking crises, the existing literature identifies inflating stock and real estate bubbles, credit booms, and surges in net capital inflows as the common drivers. Employing panel logit models to predict the postwar systemic banking crises in advanced economies to occur within three–four years, the paper’s key finding is that, even after controlling for the effects of surges in asset and credit markets and net capital inflows that are above the long-run trends for an extended period, market participants’ increasing underestimation of downside risks is a significant predictor of these crises. Incorporating changes in risk perception improves the prediction accuracy of the model significantly. This finding is robust across alternative prediction horizons, systemic crisis definitions, and risk-perception measures. Consistent with the recent theoretical developments in the form of the diagnostic expectations hypothesis for financial markets, the interpretation is that recent recurring good news about financial markets and the broader economic trends for sufficiently long periods lead to growing neglect of tail risks and riskier financial transactions, raising systemic risk and the likelihood of a financial crisis. The finding suggests monitoring financial market risk perception, in addition to the conventional indicators, to predict and avert systemic banking crises.
The Effect of Religion in European Financial Statement Disclosures: A Real Earnings’ Management Case
Authors: Toudas Kanellos S. , Zhu Jinxiu
Published: October 2023

Prior research has extensively examined the relationship between religion and accrual-based earnings management. However, there is currently little research on the relationship between religion and real (non-accrual) earnings management, especially in Europe. This paper aims to fill this research gap and examines whether and how the effect of religion could be linked with firms’ real earnings management activities. Four hypotheses are developed and tested, with our results providing indications that the degree of overall religiosity is negatively and significantly associated with real earnings management. Furthermore, when investigating the effects of different religions in Europe, Christianity and Islam have the opposite impact on firms’ real earnings management activities. Overall, our paper indicates that in European countries, the religious environment can mitigate firms’ manipulations on earnings.
Non-Pronominal Intransitive Verb Variants with Property Interpretation: A Characterization
Authors: Felíu Arquiola Elena
Published: October 2023

In this paper, we study a syntactic construction that has received little attention in the study of Spanish grammar (La masa de pizza congela perfectamente, ‘Pizza dough freezes perfectly’). It is characterized by the presence of a verb in a non-pronominal intransitive variant, with property interpretation. This construction is related to mediopassive sentences or generic middles (Las camisas de algodón se lavan fácilmente, ‘Cotton shirts wash easily’), as well as to the anticausative construction (La camisa se secó al sol, ‘The shirt dried in the sun’), a relationship that has not always been highlighted in previous studies, which are usually based on a short list of verbs. Taking as a point of departure both the verbal classification found in ADESSE and data from Corpus del Español: Web/Dialects, the construction under study is described, paying special attention to the types of verbs found in it in order to check if they coincide with the verbs found in mediopassive sentences or the anticausative construction. In addition, our study provides new data on the linguistic factors (presence of a manner adverb or other modifiers, negation), as well as on the extralinguistic factors (type of text, geographical distribution) involved in this phenomenon.
Negation That Isn’t
Authors: Gračanin-Yüksek Martina
Published: October 2023

In this paper I investigate the ne…ne construction in Turkish, illustrated by Ne Ali ne (de) Esra geldi ‘Neither Ali nor Esra arrived’. The meaning of the ne…ne construction roughly corresponds to the meaning of the neither…nor construction in English, but the syntactic properties of ne…ne are somewhat different from those of neither…nor. I focus on two such differences: one, the fact that ne…ne can, although it doesn’t have to, be accompanied by a negated verb; in fact, a negated verb is slightly dispreferred by speakers (but the presence versus the absence of negation interacts in interesting ways with negative concord); and two, the fact that the ne…ne construction cannot be embedded under a wide-scope question particle –mI except when the verb is negated.
Significantly Improving the High-Temperature Tensile Properties of Al17Cr10Fe36Ni36Mo1 Alloys by Microalloying Hf
Authors: Chen Zhihua , Wang Jianbin , Jia Yuhao , Wu Qingfeng , Liu Xiaoming , Liu Linxiang , Li Junjie , He Feng , Wang Zhijun , Wang Jincheng
Published: October 2023

Dual-phase high-entropy alloys with excellent room temperature and high-temperature properties have been widely studied as potential high-temperature structural materials. However, interface weakening causes its high-temperature performance to decline at higher temperatures, severely limiting further development. In this study, a series of Al17Cr10Fe36Ni36Mo1Hfx (x = 0, 0.03, 0.15, 0.3, 0.5, and 0.8 at%) alloys were prepared to study the effect of Hf content on the microstructure and mechanical properties of the matrix alloy. The results indicate that with the addition of the Hf, the Hf-rich phase began to precipitate at the interface and inside the B2 phase in the matrix alloy. In contrast, the morphology of both the FCC and B2 phases had no noticeable change. With the increase in Hf content, the high-temperature strength and ductility of the alloy first increased and then decreased, while the room temperature performance remained almost unchanged. Benefiting from the hindrance of the Hf-rich phase to grain boundary sliding and dislocation movement during high-temperature deformation, the tensile strength, yield strength, and plasticity of the matrix alloy increased from 474 MPa, 535 MPa, and 8.7% to 816 MPa, 923 MPa, and 42.0% for the Al17Cr10Fe36Ni36Mo1Hf0.5 alloys, respectively. This work provides a new path for designing a high-entropy alloy with excellent high-temperature mechanical properties.
Analysis of the Possibility of Using Selected Tools and Algorithms in the Classification and Recognition of Type of Microstructure
Authors: Szatkowski Michał , Wilk-Kołodziejczyk Dorota , Jaśkowiec Krzysztof , Małysza Marcin , Bitka Adam , Głowacki Mirosław
Published: October 2023

The aim of this research was to develop a solution based on existing methods and tools that would allow the automatic classification of selected images of cast iron microstructures. As part of the work, solutions based on artificial intelligence were tested and modified. Their task is to assign a specific class in the analyzed microstructure images. In the analyzed set, the examined samples appear in various zoom levels, photo sizes and colors. As is known, the components of the microstructure are different. In the examined photo, there does not have to be only one type of precipitate in each photo that indicates the correct microstructure of the same type of alloy, different shapes may appear in different amounts. This article also addresses the issue of data preparation. In order to isolate one type of structure element, the possibilities of using methods such as HOG (histogram of oriented gradients) and thresholding (the image was transformed into black objects on a white background) were checked. In order to avoid the slow preparation of training data, our solution was proposed to facilitate the labeling of data for training. The HOG algorithm combined with SVM and random forest were used for the classification process. In order to compare the effectiveness of the operation, the Faster R-CNN and Mask R-CNN algorithms were also used. The results obtained from the classifiers were compared to the microstructure assessment performed by experts.
Inequalities That Imply the Norm of a Linear Space Is Induced by an Inner Product
Authors: Rădulescu Sorin , Rădulescu Marius , Bencze Mihaly
Published: October 2023

The aim of this paper is to investigate when a linear normed space is an inner product space. Several conditions in a linear normed space are formulated with the help of inequalities. Some of them are from the literature and others are new. We prove that these conditions are equivalent with the fact that the norm is induced by an inner product. One of the new results is the following: in an inner product space, the sum of opposite edges of a tetrahedron are the sides of an acute angled triangle. The converse of this result holds also. More precisely, this property characterizes inner product spaces. Another new result is the following: in a tetrahedron, the sum of squares of opposite edges are the lengths of a triangle. We prove also that this property characterizes inner product spaces. In addition, we give simpler proofs to some theorems already known from the publications of other authors.
Competitive Coevolution-Based Improved Phasor Particle Swarm Optimization Algorithm for Solving Continuous Problems
Authors: Ali Omer , Abbas Qamar , Mahmood Khalid , Bautista Thompson Ernesto , Arambarri Jon , Ashraf Imran
Published: October 2023

Particle swarm optimization (PSO) is a population-based heuristic algorithm that is widely used for optimization problems. Phasor PSO (PPSO), an extension of PSO, uses the phase angle θ to create a more balanced PSO due to its increased ability to adjust the environment without parameters like the inertia weight w. The PPSO algorithm performs well for small-sized populations but needs improvements for large populations in the case of rapidly growing complex problems and dimensions. This study introduces a competitive coevolution process to enhance the capability of PPSO for global optimization problems. Competitive coevolution disintegrates the problem into multiple sub-problems, and these sub-swarms coevolve for a better solution. The best solution is selected and replaced with the current sub-swarm for the next competition. This process increases population diversity, reduces premature convergence, and increases the memory efficiency of PPSO. Simulation results using PPSO, fuzzy-dominance-based many-objective particle swarm optimization (FMPSO), and improved competitive multi-swarm PPSO (ICPPSO) are generated to assess the convergence power of the proposed algorithm. The experimental results show that ICPPSO achieves a dominating performance. The ICPPSO results for the average fitness show average improvements of 15%, 20%, 30%, and 35% over PPSO and FMPSO. The Wilcoxon statistical significance test also confirms a significant difference in the performance of the ICPPSO, PPSO, and FMPSO algorithms at a 0.05 significance level.
Solving Integral Equation and Homotopy Result via Fixed Point Method
Authors: Alamri Badriah
Published: October 2023

The aim of the present research article is to investigate the existence and uniqueness of a solution to the integral equation and homotopy result. To achieve our objective, we introduce the notion of (α,η,ψ)-contraction in the framework of F-bipolar metric space and prove some fixed point results for covariant and contravariant mappings. Some coupled fixed point results in F-bipolar metric space are derived as outcomes of our principal theorems. A non-trivial example is also provided to validate the authenticity of the established results.
EEG-BCI Features Discrimination between Executed and Imagined Movements Based on FastICA, Hjorth Parameters, and SVM
Authors: Mwata-Velu Tat’y , Navarro Rodríguez Armando , Mfuni-Tshimanga Yanick , Mavuela-Maniansa Richard , Martínez Castro Jesús Alberto , Ruiz-Pinales Jose , Avina-Cervantes Juan Gabriel
Published: October 2023

Brain–Computer Interfaces (BCIs) communicate between a given user and their nearest environment through brain signals. In the case of device handling, an accurate control-based BCI depends essentially on how the user performs corresponding mental tasks. In the BCI illiteracy-related literature, one subject could perform a defined paradigm better than another. Therefore, this work aims to identify recorded Electroencephalogram (EEG) signal segments related to the executed and imagined motor tasks for BCI system applications. The proposed approach implements pass-band filters and the Fast Independent Component Analysis (FastICA) algorithm to separate independent sources from raw EEG signals. Next, EEG features of selected channels are extracted using Hjorth parameters. Finally, a Support Vector Machines (SVMs)-based classifier identifies executed and imagined motor features. Concretely, the Physionet dataset, related to executed and imagined motor EEG signals, provided training, testing, and validating data. The numerical results let us discriminate between executed and imagined motor tasks accurately. Therefore, the proposed method offers a reliable alternative to extract EEG features for BCI based on executed and imagined movements.
On a Symmetric Image Cryptosystem Based on a Novel One-Dimensional Chaotic System and Banyan Network
Authors: Huang Qingye , Huang Linqing , Cai Shuting , Xiong Xiaoming , Zhang Hui
Published: October 2023

In this paper, a Banyan network with high parallelism and nonlinearity is used for the first time in image encryption to ensure high complexity and randomness in a cipher image. To begin, we propose a new 1-D chaotic system (1-DSCM) which improves the chaotic behavior and control parameters’ structure of the sin map. Then, based on 1-DSCM, a Banyan network, and SHA-256 hash function, a novel image encryption algorithm is conducted. Firstly, a parameter is calculated using SHA-256 hash function and then employed to preprocess the plaintext image to guarantee high plaintext sensitivity. Secondly, a row–column permutation operation is performed to gain the scrambled image. Finally, based on the characteristic of DNA encoding, a novel DNA mapping is constructed using an N=4 Banyan network and is used to diffuse the scrambled image. Simulation results show that the 1-DSCM has excellent performance in chaotic behavior and that our encryption algorithm exhibits strong robustness against various attacks and is suitable for use in modern cryptosystems.
Dynamic Response of Paper-Based Bi-Material Cantilever Actuator
Authors: Kumar Ashutosh , Hatayama Jun , Rahmani Nassim , Anagnostopoulos Constantine , Faghri Mohammad
Published: October 2023

This work presents a dynamic modeling approach for analyzing the behavior of a bi-material cantilever actuator structure, consisting of a strip of filter paper bonded to a strip of tape. The actuator’s response is induced by a mismatch strain generated upon wetting, leading to the bending of the cantilever. The study delves into a comprehensive exploration of the dynamic deflection characteristics of the bilayer structure. It untangles the intricate connections among the saturation, modulus, hygro-expansion strain, and deflection, while uniquely addressing the challenges stemming from fluid–structure coupling. To solve the coupled fluid–solid differential equations, a combined numerical method is employed. This involves the application of the Highly Simplified Marker and Cell (HSMAC) technique for fluid flow analysis and the Finite Difference Method (FDM) for response deflection computation. In terms of the capillary flow model, the Computational Fluid Dynamics (CFD) simulations closely align with the classical Washburn relationship, depicting the wetted front’s evolution over time. Furthermore, the numerical findings demonstrate that heightened saturation levels trigger an increase in hygro-expansion strain, consequently leading to a rapid rise in response deflection until a static equilibrium is achieved. This phenomenon underscores the pivotal interplay among saturation, hygro-expansion strain, and deflection within the system. Additionally, the actuator’s response sensitivity to material characteristics is highlighted. As the mismatch strain evolving from paper hygro-expansion diminishes, a corresponding reduction in the axial strain causes a decrease in response deflection. The dynamic parameter demonstrates that the deflection response of the bilayer actuator diminishes as dynamic pressure decreases, reaching a minimal level beyond which further changes are negligible. This intricate correlation underscores the device’s responsiveness to specific material traits, offering prospects for precise behavior tuning. The dependence of paper modulus on saturation levels is revealed to significantly influence bilayer actuator deflection. With higher saturation content, the modulus decreases, resulting in amplified deflection. Finally, strong concordance is observed among the present fluidically coupled model, the static model, and empirical data—a testament to the accuracy of the numerical formulation and results presented in this study.
Endoscopic Diagnosis and Therapy for Epstein–Barr Virus-Associated Gastric Cancer
Authors: Yanai Hideo , Fujiwara Junko , Toyama Eiichiro , Okuda Hiroshi , Miura Osamu , Kaino Seiji , Nishikawa Jun
Published: October 2023

Epstein-Barr-virus-associated gastric cancer (EBVaGC) represents almost 7% of all GC and is a distinct subtype of GC with extreme DNA hypermethylation. EBVaGC is a tumor-infiltrating lymphocyte-rich tumor with little lymph-node metastasis in its early stage and with a relatively favorable prognosis in its advanced stage. Using upper gastrointestinal endoscopy, we recognize EBVaGC as a mainly depressed type with SMT-like protrusion in the upper part of the stomach near the gastric mucosal atrophic border or remnant stomach. The EBVaGC recognition rate of 21.4% with the endoscopic motif is not high, and further progress in endoscopic diagnosis of EBVaGC is needed. As less invasive endoscopic therapy, the extension of the criteria of endoscopic submucosal dissection (ESD) for early EBVaGC with little lymph-node metastasis should be discussed. Endoscopic diagnosis of EBVaGC may be relevant for the selection of patients who could benefit from endoscopic treatment or chemotherapy.
Effects of Maize–Crop Rotation on Soil Physicochemical Properties, Enzyme Activities, Microbial Biomass and Microbial Community Structure in Southwest China
Authors: Wang Puchang , Xie Wenhui , Ding Leilei , Zhuo Yingping , Gao Yang , Li Junqin , Zhao Lili
Published: October 2023

Introducing cover crops into maize rotation systems is widely practiced to increase crop productivity and achieve sustainable agricultural development, yet the potential for crop rotational diversity to contribute to environmental benefits in soils remains uncertain. Here, we investigated the effects of different crop rotation patterns on the physicochemical properties, enzyme activities, microbial biomass and microbial communities in soils from field experiments. Crop rotation patterns included (i) pure maize monoculture (CC), (ii) maize–garlic (CG), (iii) maize–rape (CR) and (iv) maize–annual ryegrass for one year (Cir1), two years (Cir2) and three years (Cir3). Our results showed that soil physicochemical properties varied in all rotation patterns, with higher total and available phosphorus concentrations in CG and CR and lower soil organic carbon and total nitrogen concentrations in the maize–ryegrass rotations compared to CC. Specifically, soil fertility was ranked as CG > Cir2 > CR > Cir3 > CC > Cir1. CG decreased enzyme activities but enhanced microbial biomass. Cir2 decreased carbon (C) and nitrogen (N) acquiring enzyme activities and soil microbial C and N concentrations, but increased phosphorus (P) acquiring enzyme activities and microbial biomass P concentrations compared to CC. Soil bacterial and fungal diversity (Shannon index) were lower in CG and Cir2 compared to CC, while the richness (Chao1 index) was lower in CG, CR, Cir1 and Cir2. Most maize rotations notably augmented the relative abundance of soil bacteria, including Chloroflexi, Gemmatimonadetes and Rokubacteria, while not necessarily decreasing the abundance of soil fungi like Basidiomycota, Mortierellomycota and Anthophyta. Redundancy analysis indicated that nitrate-N, ammonium-N and microbial biomass N concentrations had a large impact on soil bacterial communities, whereas nitrate-N and ammonium-N, available P, soil organic C and microbial biomass C concentrations had a greater effect on soil fungal communities. In conclusion, maize rotations with garlic, rape and ryegrass distinctly modify soil properties and microbial compositions. Thus, we advocate for garlic and annual ryegrass as maize cover crops and recommend a two-year rotation for perennial ryegrass in Southwest China.
Phylogenetic Analysis and Emerging Drug Resistance against Different Nucleoside Analogues in Hepatitis B Virus Positive Patients
Authors: Gohar Maryam , Rehman Irshad Ur , Ullah Amin , Khan Muhammad Ajmal , Yasmin Humaira , Ahmad Jamshaid , Butt Sadia , Ahmad Ajaz
Published: October 2023

Several nucleotide analogues have been approved for use in treating hepatitis B virus (HBV) infection. Long-term exposure to therapy leads to the emergence of mutations within the HBV DNA polymerase gene, resulting in drug resistance, a major factor contributing to therapy failure. Chronic HBV patients from the Khyber Pakhtunkhwa province, Pakistan, who had completed 6 months of therapy participated in this study. Samples were collected from 60 patients. In this study, the entire reverse transcriptase domain of the HBV polymerase gene was amplified using nested polymerase chain reaction and sequenced. Drug-resistant mutations were detected in nine (22.5%) patients. All of these patients had lamivudine-resistant mutations (rtM204V + L180M), while seven individuals (17.5%) had both lamivudine- plus entecavir-resistant mutations (L180M + M204V + S202G). N236T, a mutation that gives rise to tenofovir and adefovir resistance, was observed in two (5%) patients. T184A, a partial drug-resistant mutation to entecavir, was found in five (12.5%) patients. Furthermore, other genotypic variants (100%) and vaccine escape mutations (5%) were additionally observed. Moreover, pN459Y (35%), pN131D (20%), pL231S (20%), pP130Q (17.5%), pS189Q (12.5%), pP161S (5%), pH160P (2.5%), pT322S (2.5%), and pA223S (2.5%) mutations in the polymerase gene, as well as sA166V (17.5%), sQ181K (12.5%), sV184R (7.5%), sA17E (5%), sP153S/K (5%), sW156C (5%), sC76Y (2.5%), and S132F (2.5%) mutations in the small surface gene, were identified for the first time in this study. Phylogenetic analysis showed that genotype D was predominant amongst the HBV carriers. Subtype D1 was found in most patients, while two patients were subtype D9. These novel findings may contribute to the body of knowledge and have clinical significance for treating and curing HBV infections in Pakistan.
Re-Os Systematics in the Layered Rocks and Cu-Ni-PGE Sulfide Ores from the Dovyren Intrusive Complex in Southern Siberia, Russia: Implications for the Original Mantle Source and the Effects of Two-Stage Crustal Contamination
Authors: Ariskin Alexey A. , Tessalina Svetlana G. , Kostitsyn Yuri A. , Pshenitsyn Ivan V. , Sobolev Sergei N. , Nikolaev Georgy S. , Kislov Evgeny V.
Published: October 2023

The Dovyren Intrusive Complex (Northern Baikal region, 728 ± 3 Ma) includes the dunite–troctolite–gabbronorite Yoko–Dovyren massif (YDM) associated with a sequence of underlying mafic-to-ultramafic sills, locally demonstrating interbedding relations with the most primitive rocks of the pluton. These sills and apophyses contain sulfide mineralization ranging from globular to net-textured and massive ores. Major types of the YDM cumulates and sulfide mineralization were examined for their PGE contents and Re-Os isotopic systematics. The ten analyzed samples included chilled and basal rocks, poorly mineralized troctolite, PGE-rich anorthosite, as well as three samples from a thick ore-bearing apophysis DV10 connected with the YDM. These samples yielded a Re-Os isochron with an age of 759 ± 36 Ma and an initial 187Os/188Os of 0.1309 ± 0.0026 (MSWD = 110), which is in consistent with the previously reported U–Pb zircon age. It is shown that being recalculated to γOs(t) at t = 728 Ma, these isotopic compositions demonstrate three clusters regarding the relationship between γOs(t) and 187Re/188Os: (i) the chilled gabbronorite (YDM) and subcontact olivine gabbronorite (DV10) yielded the most radiogenic values of γOs(t) 10.5 and 10.0 among basal ultramafics, (ii) plagiodunite, troctolite, and sulfide ores showed lower radiogenic compositions, with γOs(t) ranging from 7.3 to 8.7, (iii) olivine gabbronorite, plagioperidotite, and one sample of PGE-rich anorthosite yield very primitive γOs(t) in the range 4.5 to 5.6 (on average 5.2 ± 0.6). The lowest values of γOs(t) for the least fractionated rocks of the YDM suggest a primitive mantle source, formed from a partly contaminated Neoarchean protolith, which is considered to be anomalous in Upper Riphean due to very low εNd(t) of −16 for the most primitive Dovyren magma (Fo88-parent). The highest values of γOs(t) and relative enrichment in the 34S isotope in the chilled gabbronorite (YDM) and subcontact olivine gabbronorite (DV10) evidence that their primitive to evolved magmatic precursors could be affected by a metamorphic fluid enriched in radiogenic 187Os, originating in the exocontact halo due to the thermal decomposition of pyrite from the dehydrated country rocks. This is consistent with the second-stage contamination of the Dovyren magma by the hosting crustal rocks (probably of 10 wt% shists), generating more evolved Fo86-parent magma with higher εNd(t) of −14.
Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment
Authors: Shen Ming-Hung , Liu Chih-Yi , Chang Kang-Wei , Lai Ching-Long , Chang Shih-Chang , Huang Chi-Jung
Published: October 2023

Colorectal cancer (CRC) is one of the most common cancers and is the second leading cause of cancer-related death in the world. Due to the westernization of diets, young patients with CRC are often diagnosed at advanced stages with an associated poor prognosis. Improved lifestyle choices are one way to minimize CRC risk. Among diet choices is the inclusion of bee propolis, long recognized as a health supplement with anticancer activities. Understanding the effect of propolis on the gut environment is worth exploring, and especially its associated intratumoral immune changes and its anticancer effect on the occurrence and development of CRC. In this study, early stage CRC was induced with 1,2-dimethylhydrazine (DMH) and dextran sulfate sodium (DSS) for one month in an animal model, without and with propolis administration. The phenotypes of early stage CRC were evaluated by X-ray microcomputed tomography and histologic examination. The gut immunity of the tumor microenvironment was assessed by immunohistochemical staining for tumor-infiltrating lymphocytes (TILs) and further comparative quantification. We found that the characteristics of the CRC mice, including the body weight, tumor loading, and tumor dimensions, were significantly changed due to propolis administration. With further propolis administration, the CRC tissues of DMH/DSS-treated mice showed decreased cytokeratin 20 levels, a marker for intestinal epithelium differentiation. Additionally, the signal intensity and density of CD3+ and CD4+ TILs were significantly increased and fewer forkhead box protein P3 (FOXP3) lymphocytes were observed in the lamina propria. In conclusion, we found that propolis, a natural supplement, potentially prevented CRC progression by increasing CD3+ and CD4+ TILs and reducing FOXP3 lymphocytes in the tumor microenvironment of early stage CRC. Our study could suggest a promising role for propolis in complementary medicine as a food supplement to decrease or prevent CRC progression.
Repeatability of Ad Libitum Water Intake during Repeated 1 h Walking/Jogging Exercise Sessions Conducted under Hot Ambient Conditions
Authors: Goulet Eric D. B. , Claveau Pascale , Simoneau Ivan L. , Deshayes Thomas A. , Jolicoeur-Desroches Antoine , Aloui Fedi , Hoffman Martin D.
Published: October 2023

A drinking strategy aiming to replace a given percentage of the sweat losses incurred during exercise should result in reproducible fluid intake volume and, hence, fluid balance from one exercise session to the other performed under similar scenarios. Whether this may also be the case with ad libitum drinking during exercise is unclear. We characterized the repeatability of ad libitum water intake during repeated 1 h exercise sessions and examined its effect over time on fluid balance and selected physiological functions and perceptual sensations. Twelve (3 women) healthy individuals participated in this study. At weekly intervals, they completed four 2 × 30 min walking/jogging exercise bouts (55% V˙O2max, 40 °C, 20–30% relative humidity) interspersed by a 3 min recovery period. During exercise, participants consumed water (20 °C) ad libitum. There were no significant differences among the four exercise sessions for absolute water intake volume (~1000 mL·h−1), percent body mass loss (~0.4%), sweat rate (~1300 mL·h−1) and percent of sweat loss replaced by water intake (~80%). Heart rate, rectal temperature, and perceived thirst and heat stress did not differ significantly between the first and fourth exercise sessions. Perceived exertion was significantly lower during the fourth vs. the first exercise session, but the difference was trivial (<1 arbitrary unit). In conclusion, ad libitum water intake during four successive identical 1 h walking/jogging sessions conducted in the heat will result in similar water intake volumes and perturbations in fluid balance, heart rate, rectal temperature, and perceived thirst, heat stress and exertion.