bluuebunny/crossref_metadata_2025 huggingface dataset:
Ubiquitin-Conjugating Enzymes in Cancer
Authors: Bui Quyen Thu , Hong Jeong Hee , Kwak Minseok , Lee Ji Yeon , Lee Peter Chang-Whan
Published: June 2021

The ubiquitin-mediated degradation system is responsible for controlling various tumor-promoting processes, including DNA repair, cell cycle arrest, cell proliferation, apoptosis, angiogenesis, migration and invasion, metastasis, and drug resistance. The conjugation of ubiquitin to a target protein is mediated sequentially by the E1 (activating)‒E2 (conjugating)‒E3 (ligating) enzyme cascade. Thus, E2 enzymes act as the central players in the ubiquitination system, modulating various pathophysiological processes in the tumor microenvironment. In this review, we summarize the types and functions of E2s in various types of cancer and discuss the possibility of E2s as targets of anticancer therapeutic strategies.
Asthmatic Eosinophils Promote Contractility and Migration of Airway Smooth Muscle Cells and Pulmonary Fibroblasts In Vitro
Authors: Janulaityte Ieva , Januskevicius Andrius , Kalinauskaite-Zukauske Virginija , Palacionyte Jolita , Malakauskas Kestutis
Published: June 2021

Enhanced contractility and migration of airway smooth muscle cells (ASMC) and pulmonary fibroblasts (PF) are part of airway remodeling in asthma. Eosinophils are the central inflammatory cells that participate in airway inflammation. However, the role of asthmatic eosinophils in ASMC and PF contractility, migration, and differentiation to contractile phenotype has not yet been precisely described. A total of 38 individuals were included in this study: 13 steroid-free non-severe allergic asthma (AA) patients, 11 severe non-allergic eosinophilic asthma (SNEA) patients, and 14 healthy subjects (HS). For AA patients and HS groups, a bronchial allergen challenge with D. pteronyssinus was performed. Individual combined cell cultures were prepared from isolated peripheral blood eosinophils and immortalized ASMC or commercial PF cell lines separately. The migration of ASMC and PF was evaluated using wound healing assay and contractility using collagen gel assay. Gene expression of contractile apparatus proteins, COL1A1, COL5A1, and FN, in ASMC and PF was evaluated using qRT-PCR. We found that contractility and migration of ASMC and PF significantly increased after incubation with asthmatic eosinophils compared to HS eosinophils, p < 0.05, and SNEA eosinophils demonstrated the highest effect on contractility of ASMC and migration of both cell lines, p < 0.05. AA and SNEA eosinophils significantly increased gene expression of contractile apparatus proteins, COL1A1 and FN, in both cell lines, p < 0.05. Furthermore, the allergen-activated AA eosinophils significantly increased the contractility of ASMC, and migration and gene expression in ASMC and PF, p < 0.05. Thus, asthmatic eosinophils change ASMC and PF behavior by increasing their contractility and migration, contributing to airway remodeling.
Thermally Activated Al(OH)3: Part I—Morphology and Porosity Evaluation
Authors: Vasile Bogdan Stefan , Dobra Gheorghe , Iliev Sorin , Cotet Lucian , Neacsu Ionela Andreea , Nicoara Adrian Ionut , Surdu Vasile Adrian , Boiangiu Alina , Filipescu Laurențiu
Published: June 2021

Aluminum hydroxide is an essential material for the industrial production of ceramics (especially insulators and refractories), desiccants, absorbents, flame retardants, filers for plastics and rubbers, catalysts, and various construction materials. The calcination process of Al(OH)3 first induces dehydration and, finally, results in α-Al2O3 formation. Nevertheless, this process contains various intermediary steps and has been proven to be complicated due to the development of numerous transitional alumina. Each step of the investigation is vital for the entire process because the final properties of materials based on aluminum trihydroxide are determined by their phase composition, morphology, porosity, etc. In this paper, five dried, milled, and size-classified aluminum hydroxide specimens were thermally treated at 260, 300, and 400 °C; then, they were studied in order to identify the effects of temperature on their properties, such as particle morphology, specific surface area, pore size, and pore distribution. The major oxide compounds identified in all samples were characteristic of bauxite—namely, Al2O3 * 3H2O, SiO2, Fe2O3, Na2O, and CaO. Particles with smaller sizes (<10 µm = 76.28%) presented the highest humidity content (~5 wt.%), while all samples registered a mass loss of ~25 wt.% on ignition at 400 °C. The identified particles had the shapes of hexagonal or quasi-hexagonal platelets and resulted in large spherulitic concretions. The obtained results suggest that ceramic powders calcined at 400 °C should be used for applications as adsorbents or catalysts due to their high specific area of about 200–240 m2/g and their small pore width (3–3.5 nm).
A New Sterilization Strategy Using TiO2 Nanotubes for Production of Free Radicals that Eliminate Viruses and Application of a Treatment Strategy to Combat Infections Caused by Emerging SARS-CoV-2 during the COVID-19 Pandemic
Authors: Hamza Reham Z. , Gobouri Adil A. , Al-Yasi Hatim M. , Al-Talhi Tarek A. , El-Megharbel Samy M.
Published: June 2021

Synthesized titanium oxide nanoparticles (TiO2-NPs) nanotubes were used for the disinfection of new emerging corona virus-19 (SARS-CoV-2) in this study. The newly synthesized TiO2-NPs (nanotubes) were characterized by chemical spectroscopic analysis Fourier-transform infrared spectroscopy and ultraviolet FT-IR and UV. The chemical purity and Zeta potential distribution of the TiO2-NPs (nanotubes) were evaluated to confirm their nano-range, and their surface morphology was determined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and energy dispersive X-ray analysis (EDX). The antiviral activity of the TiO2-NPs (nanotubes) against SARS-CoV-2 was evaluated using 10% (Dimethyl sulfoxide) DMSO and dist.H2O using a cytotoxicity assay and inhibitory concentration assay (to determine the cytotoxic half concentration CC50 and half maximal inhibitory concentration IC50). The current results confirmed that TiO2-NPs exhibit strong anti-SARS-CoV-2 activity at very low cytotoxic concentrations in vitro with a non-significant selectivity index (CC50/IC50 ≤ 1). The obtained results indicate that TiO2-NPs and nanotubes have potent antiviral activity at a very low concentrations (IC50 = 568.6 ng/mL), with a weak cytotoxic effect on the cellular host (CC50 = 399.1 ng/mL). Thus, we highly recommend the use of TiO2-NPs (nanotubes) in vitro and in wall coatings as a potent disinfectant to combat SARS-CoV-2 with little irritation of the cellular hosts. Furthermore, we also recommend more and excessive prospective studies on the complexation of natural active or natural compounds with TiO2-NPs (nanotubes) to minimize their cytotoxicity, enhance their antiviral activity, and increase their inhibition of SARS-CoV-2.
Modeling and Reconstruction Strategy for Compton Scattering Tomography with Scintillation Crystals
Authors: Kuger Lorenz , Rigaud Gael
Published: June 2021

The recent development of energy-resolved scintillation crystals opens the way to build novel imaging concepts based on the variable energy. Among them, Compton scattering tomography (CST) is one of the most ambitious concepts. Akin to Computerized Tomography (CT), it consists in probing the attenuation map of an object of interest using external ionizing sources but strives to exploit the scattered radiation as an imaging agent. For medical applications, the scattered radiation represents 70 to 80% when the energy of the source is larger than 100 keV and results from the Compton effect. This phenomenon stands for the collision of a photon with an electron and rules the change of course and loss of energy undergone by the photon. In this article, we propose a modeling for the scattered radiation assuming polychromatic sources such as 60Co and scintillation crystals such as LBC:Ce. Further, we design a general strategy for reconstructing the electron density of the target specimen. Our results are illustrated for toy objects.
The Screening and COnsensus Based on Practices and Evidence (SCOPE) Program–Results of a Survey on Daily Practice Patterns for Patients with mCRC
Authors: Prager Gerald , Köhne Claus-Henning , O’Connor Juan Manuel , Rivera Fernando , Santini Daniele , Wasan Harpreet , Phelip Jean Marc
Published: June 2021

The SCOPE project aimed to better understand practice patterns, identify drivers for treatment goals, and determine third- and fourth-line treatment choices for patients with metastatic colorectal cancer (mCRC). The survey was developed by an expert panel of gastrointestinal oncologists. Questions concerned general practice patterns, and treatment decisions for three hypothetical patient case scenarios. Participants had to routinely manage patients with mCRC. We present results from 629 participants who provided input on patient treatment scenarios (data cutoff: 17/01/2020). Prolonging overall survival (OS; 51%) was the main aim in first line. In third line, quality of life (QOL) was the primary goal (34%). Forty-three percent also cited efficacy-focused goals; 18% and 13% noted prolonging OS and improving progression-free survival as main aims, respectively. For fit and active patients, 89% of respondents considered trifluridine-tipiracil an appropriate third-line treatment; regorafenib (31%) or clinical trial enrollment (29%) were the fourth-line options. For patients with comorbidities and limited caregiver support, trifluridine-tipiracil was the preferred third-line treatment (70%). For KRAS-mutated patients with comorbidities and adverse events who received prior oxaliplatin, 90% considered oxaliplatin rechallenge an unsuitable third-line treatment, mainly due to the risk of cumulative toxicity (75%). In the third/fourth-line settings, trifluridine-tipiracil followed by regorafenib was the most common option (54%); 17% chose regorafenib followed by trifluridine-tipiracil. Efficacy coupled with QOL are important goals in third-line treatment. Daily practice patterns reflect the guideline recommendations in third- and fourth-line settings, with a trend toward using trifluridine-tipiracil versus regorafenib in KRAS-wildtype and KRAS-mutant tumors.
Generating Component Designs for an Improved NVH Performance by Using an Artificial Neural Network as an Optimization Metamodel
Authors: von Wysocki Timo , Rieger Frank , Tsokaktsidis Dimitrios Ernst , Gauterin Frank
Published: June 2021

In modern vehicle development, suspension components have to meet many boundary conditions. In noise, vibration, and harshness (NVH) development these are for example eigenfrequencies and frequency response function (FRF) amplitudes. Component geometry parameters, for example kinematic hard points, often affect multiple of these targets in a non intuitive way. In this article, we present a practical approach to find optimized parameters for a component design, which fulfills an FRF target curve. By morphing an initial component finite element model we create training data for an artificial neural network (ANN) which predicts FRFs from geometry parameter input. Then the ANN serves as a metamodel for an evolutionary algorithm optimizer which identifies fitting geometry parameter sets, meeting an FRF target curve. The methodology enables a component design which considers an FRF as a component target. In multiple simulation examples we demonstrate the capability of identifying component designs modifying specific eigenfrequency or amplitude features of the FRFs.
Early Vascular and Functional Changes after Vitreoretinal Surgery: A Comparison between the Macular Hole and Epiretinal Membrane
Authors: D’Aloisio Rossella , Carpineto Paolo , Aharrh-Gnama Agbéanda , Iafigliola Carla , Cerino Luca , Di Nicola Marta , Porreca Annamaria , Toto Lisa , Mastropasqua Rodolfo
Published: June 2021

(1) Background: The aim of this observational comparative study was to investigate early retinal vascular and functional changes in patients undergoing vitreoretinal surgery for idiopathic epiretinal membrane (iERM) or macular hole (MH) using a widefield swept-source optical coherence tomography angiography (WSS-OCTA). (2) Methods: Forty one diseased eyes were enrolled in the study. Twenty three eyes with iERM diagnosis (ERM group) underwent 25-gauge vitrectomy with inner limiting membrane (ILM) and MER peeling, while eighteen eyes with MH (MH group) underwent 25-gauge vitrectomy with inverted flap technique. Functional and anatomical/perfusion parameters were evaluated pre- and postoperatively in all eyes by means of WSS-OCTA system, microperimetry (MP3), best corrected visual acuity assessment, central macular thickness (CMT) and MH diameter calculation. For each eye, 12 × 12 mm OCTA volume scans were acquired by a retinal specialist and a semi-automated algorithm was used for a quantitative vessel analysis of the superficial capillary plexus (SCP), deep capillary plexus (DCP) and choriocapillaris (CC). In detail, perfusion density (PD) of the SCP, DCP and CC was evaluated in four circles (one central in the macular area of 5 mm diameter; three midperiphery circles (temporal, superior and inferior) of 3 mm). In addition, the vessel length density (VLD) of the SCP and DCP for the same circles was quantified. (3) Results: In the MH group, PD of the SCP significantly increased in the macular area (p = 0.018) and in the superior ring (p = 0.016); PD of the DCP significantly increased in the macular area (p = 0.015) and in the superior and inferior ring (p = 0.016) 3 months after surgery. In the ERM group, PD of the SCP and DCP significantly increased in the macular area and superior ring, respectively (p = 0.001; p = 0.032), 3 months after surgery. During follow-up there was a significant improvement in terms of functional (Best corrected visual acuity, p = 0.007 and p = 0.029; microperimetry ((MP3) 10°, p = 0.003 and p = 0.004; MP3 2°, p = 0.028 and p = 0.003 in MH group and ERM group respectively) and anatomical parameters (CMT, p = 0.049 in ERM group; hole complete closure in MH group). (4) Conclusions: After vitreoretinal surgery, early retinal vascular and functional changes can be promptly observed and quantified to monitor and potentially predict surgery outcomes. Widefield OCTA devices allow for a detailed microvasculature analysis of retina and choriocapillaris in the macular area and in the periphery, showing a different behaviour of retinal sectors in two distinct vitreoretinal disorders.
Reply to Ferrés-Padró et al. Comment on “Lionte et al. Association of Multiple Glycemic Parameters at Hospital Admission with Mortality and Short-Term Outcomes in Acutely Poisoned Patients. Diagnostics 2021, 11, 361”
Authors: Sorodoc Victorita , Lionte Catalina , Bologa Cristina , Petris Ovidiu Rusalim , Sorodoc Laurentiu
Published: June 2021

Thank you for the opportunity to respond to the issues raised by Ferrés-Padró et al. in their recent letter to the editor [...]
Generalized Arterial Calcification of Infancy Type 1 (GACI1): Identification of a Novel Pathogenic Variant (c.1715T>C (p.Leu572Ser))
Authors: Bulfamante Gaetano Pietro , Carpenito Laura , Bragantini Emma , Graziani Silvia , Bellizzi Maria , Bagowski Christoph Peter , Shoukier Moneef , Rivieri Francesca , Soffiati Massimo , Barbareschi Mattia
Published: June 2021

Generalized Arterial Calcification of Infancy (GACI) is a rare disease inherited in a recessive manner, with severe and diffuse early onset of calcifications along the internal elastic lamina in large and medium size arteries. The diagnosis results are from clinical manifestations, imaging, histopathologic exams, and genetic tests. GACI is predominantly caused by biallelic pathogenic variant in the ENPP1 gene (GACI1, OMIM#208000) and, to a lesser extent, by pathogenic variants in the ABCC6 gene (GACI2, OMIM#614473). We present a novel variation in the ENPP1 gene identified in a patient clinically diagnosed with GACI and confirmed by genetic investigation and autopsy as GACI type 1. The sequence analysis of the patient’s ENPP1 gene detected two heterozygous variants c.1412A>G (p.Tyr471Cys) and c.1715T>C (p.Leu572Ser). The variant c.1715T>C (p.Leu572Ser) has not been described yet in the literature and in mutation databases. A genetic analysis was also carried out for the parents of the newborn; the heterozygous pathogenic variant c.1412A>G (p.Tyr471Cys) was detected in the mother’s ENPP1 gene, and a sequence analysis of the father’s ENPP1 gene revealed the novel heterozygous variant c.1715T>C (p.Leu572Ser). Our results showed that the variant c.1715T>C (p.Leu572Ser) may have a pathogenic role in the development of GACI type1 (GACI1, OMIM#208000), at least when associated with the pathogenic c.1412A>G (p.Tyr471Cys) variant. The identification of novel mutations potentially enabled genotype/phenotype associations that will ultimately have an impact on clinical management and prognosis for the disease.
A Geometric Perspective on Information Plane Analysis
Authors: Basirat Mina , Geiger Bernhard C. , Roth Peter M.
Published: June 2021

Information plane analysis, describing the mutual information between the input and a hidden layer and between a hidden layer and the target over time, has recently been proposed to analyze the training of neural networks. Since the activations of a hidden layer are typically continuous-valued, this mutual information cannot be computed analytically and must thus be estimated, resulting in apparently inconsistent or even contradicting results in the literature. The goal of this paper is to demonstrate how information plane analysis can still be a valuable tool for analyzing neural network training. To this end, we complement the prevailing binning estimator for mutual information with a geometric interpretation. With this geometric interpretation in mind, we evaluate the impact of regularization and interpret phenomena such as underfitting and overfitting. In addition, we investigate neural network learning in the presence of noisy data and noisy labels.
Switching and Swapping of Quantum Information: Entropy and Entanglement Level
Authors: Sawerwain Marek , Wiśniewska Joanna , Gielerak Roman
Published: June 2021

Information switching and swapping seem to be fundamental elements of quantum communication protocols. Another crucial issue is the presence of entanglement and its level in inspected quantum systems. In this article, a formal definition of the operation of the swapping local quantum information and its existence proof, together with some elementary properties analysed through the prism of the concept of the entropy, are presented. As an example of the local information swapping usage, we demonstrate a certain realisation of the quantum switch. Entanglement levels, during the work of the switch, are calculated with the Negativity measure and a separability criterion based on the von Neumann entropy, spectral decomposition and Schmidt decomposition. Results of numerical experiments, during which the entanglement levels are estimated for systems under consideration with and without distortions, are presented. The noise is generated by the Dzyaloshinskii-Moriya interaction and the intrinsic decoherence is modelled by the Milburn equation. This work contains a switch realisation in a circuit form—built out of elementary quantum gates, and a scheme of the circuit which estimates levels of entanglement during the switch’s operating.
Advancing Wind Resource Assessment in Complex Terrain with Scanning Lidar Measurements
Authors: Gottschall Julia , Papetta Alkistis , Kassem Hassan , Meyer Paul Julian , Schrempf Linda , Wetzel Christian , Becker Johannes
Published: June 2021

The planning and realization of wind energy projects requires an as accurate and precise wind resource estimation as possible. Standard procedures combine shorter on-site measurements with the application of numerical models. The uncertainties of the numerical data generated from these models are, particularly in complex onshore terrain, not just rather high but typically not well quantified. In this article we propose a methodology for using a single scanning Doppler wind lidar device to calibrate the output data of a numerical flow model and with this not just quantify but potentially also reduce the uncertainties of the final wind resource estimate. The scanning lidar is configured to perform Plan Position Indicator (PPI) scans and the numerical flow data are projected onto this geometry. Deviations of the derived from the recorded line-of-sight wind speeds are used to identify deficiencies of the model and as starting point for an improvement and tuning. The developed methodology is demonstrated based on a study for a site in moderately complex terrain in central Germany and using two rather different types of numerical flow models. The findings suggest that the use of the methodology and the introduced scanning wind lidar technology offers a promising opportunity to control the uncertainty of the applied flow models, which can otherwise only be estimated very roughly.
Energy and Water Policies in Chile, Two Different Endings with Implications in the Water-Energy Nexus
Authors: Bórquez Roxana , Fuster Rodrigo
Published: June 2021

Energy and water have faced important levels of conflicts in the last 20–25 years in Chile. However, the way that they have been politically addressed in the last decade differs. These differences emerge from how these fields have been historically configurated, impacting on how the policy problems and policy options have been framed. Using thematic analysis of 93 interviews and documentary analysis, this article analyzes by contrasting two participatory processes which nourish the formulation of the energy and water policies in Chile in 2014–2015. It seeks to understand the factors that may influence why the development, impact and inclusion of new voices in public policies related to water and energy have been different, and how that can impact the water–energy nexus. Five factors emerge as determinants in this difference: structure of use, number of actors, governance and institutional framework, elite conformation, and legal framework. These factors impacted the policy processes and the scope of the policy outcomes, generating two different results: a long-term energy policy, and a water policy that did not survive the presidential period. Thus, the water–energy nexus is under pressure as a result of the tension between power structures, social responses to environmental issues and decision-making, environmental limitations, and climate change stressors, creating greater vulnerability and conflicts.
Voltage Regulation For Residential Prosumers Using a Set of Scalable Power Storage
Authors: Torres Igor Cavalcante , Farias Daniel M. , Aquino Andre L. L. , Tiba Chigueru
Published: June 2021

Among the electrical problems observed from the solar irradiation variability, the electrical energy quality and the energetic dispatch guarantee stand out. The great revolution in batteries technologies has fostered its usage with the installation of photovoltaic system (PVS). This work presents a proposition for voltage regulation for residential prosumers using a set of scalable power batteries in passive mode, operating as a consumer device. The mitigation strategy makes decisions acting directly on the demand, for a storage bank, and the power of the storage element is selected in consequence of the results obtained from the power flow calculation step combined with the prediction of the solar radiation calculated by a recurrent neural network Long Short-Term Memory (LSTM) type. The results from the solar radiation predictions are used as subsidies to estimate, the state of the power grid, solving the power flow and evidencing the values of the electrical voltages 1-min enabling the entry of the storage device. In this stage, the OpenDSS (Open distribution system simulator) software is used, to perform the complete modeling of the power grid where the study will be developed, as well as simulating the effect of the overvoltages mitigation system. The clear sky day stored 9111 Wh/day of electricity to mitigate overvoltages at the supply point; when compared to other days, the clear sky day needed to store less electricity. On days of high variability, the energy stored to regulate overvoltages was 84% more compared to a clear day. In order to maintain a constant state of charge (SoC), it is necessary that the capacity of the battery bank be increased to meet the condition of maximum accumulated energy. Regarding the total loading of the storage system, the days of low variability consumed approximately 12% of the available capacity of the battery, considering the SoC of 70% of the capacity of each power level.
Rotor Current Feedback Based Direct Power Control of a Doubly Fed Induction Generator Operating with Unbalanced Grid
Authors: Pura Piotr , Iwański Grzegorz
Published: June 2021

The paper presents a decoupled direct power-controlled (DPC) of a doubly fed induction generator (DFIG) connected to an unbalanced power grid, executed in an orthogonal stationary reference frame related to the stator side. The control allows to maintain constant electromagnetic torque despite stator connection to the unbalanced power grid and to achieve controllable low-voltage ride through (LVRT) capability. The control does not require any signal decomposition into positive and negative sequence order, which allows to reduce its complexity and computational requirements. Then, the paper presents positive influence of rotor current feedback in the stator-controlled reference frame on control performance. It facilitates significant transient reduction and minor steady state control improvement. All the mentioned functionalities were validated both in simulation and in laboratory conditions and the obtained results are described in the paper.
Artificial Neural Network Simulation of Energetic Performance for Sorption Thermal Energy Storage Reactors
Authors: Delmarre Carla , Resmond Marie-Anne , Kuznik Frédéric , Obrecht Christian , Chen Bao , Johannes Kévyn
Published: June 2021

Sorption thermal heat storage is a promising solution to improve the development of renewable energies and to promote a rational use of energy both for industry and households. These systems store thermal energy through physico-chemical sorption/desorption reactions that are also termed hydration/dehydration. Their introduction to the market requires to assess their energy performances, usually analysed by numerical simulation of the overall system. To address this, physical models are commonly developed and used. However, simulation based on such models are time-consuming which does not allow their use for yearly simulations. Artificial neural network (ANN)-based models, which are known for their computational efficiency, may overcome this issue. Therefore, the main objective of this study is to investigate the use of an ANN model to simulate a sorption heat storage system, instead of using a physical model. The neural network is trained using experimental results in order to evaluate this approach on actual systems. By using a recurrent neural network (RNN) and the Deep Learning Toolbox in MATLAB, a good accuracy is reached, and the predicted results are close to the experimental results. The root mean squared error for the prediction of the temperature difference during the thermal energy storage process is less than 3K for both hydration and dehydration, the maximal temperature difference being, respectively, about 90K and 40K.
Computational Flow Analysis on a Real Scale Run-of-River Archimedes Screw Turbine with a High Incline Angle
Authors: Edirisinghe Dylan Sheneth , Yang Ho-Seong , Kim Min-Sung , Kim Byung-Ha , Gunawardane Sudath Prasanna , Lee Young-Ho
Published: June 2021

The Archimedes screw turbine (AST) is the most sustainable mini-hydropower extraction method that offers number of economic, social, and environmental advantages. Nowadays, many researchers are interested in AST development as it is considered a new technology. Currently, a lot of researchers are conducting experimental testing of the screws, comparing their reliability with computational fluid dynamic (CFD) analyses. Almost all of them are lab-scale testing models that claiming an average 80% efficiency for low pitch angles. In the case of a real site with a small inclination angle, the length of the screw is large enough to cause severe problems, specially related to bending of the screw. Therefore, this research was conducted to analyze the CFD flow field in a real site-scale AST with the maximum possible inclination of 45 degrees. In addition, the design was done without the upper and lower reservoir as it was conceived as a run-of-river flow system. The simulated real scale AST result showed a maximum efficiency of around 82% for a 5.2 m hydraulic head and 0.232 m3/s discharge. Many researchers claim above 80% efficiency for low inclination angle ASTs with reservoirs. This CFD study indicates that even higher inclination angle ASTs can achieve 80% efficiency in run-of-river; real-scale applications.
Experimental and CFD Investigation on the Application for Aerogel Insulation in Buildings
Authors: Golder Santu , Narayanan Ramadas , Hossain Md. Rashed , Islam Mohammad Rofiqul
Published: June 2021

Reducing building energy consumption is a significant challenge and is one of the most important research areas worldwide. Insulation will help to keep the building’s desired temperature by reducing the heat flow. Additionally, proper insulation can provide an extended period of comfort, leading to reduced building energy requirements. Encapsulated air is the major aspect of most thermal insulation materials. Low thermal conductivity is a good characteristic of thermal insulation materials. Aerogel has low thermal conductivity, so it is suitable for glazing and insulation purposes. This research paper investigates the effectiveness of aerogel as an insulation material in buildings by incorporating a translucent aerogel-glazing system in the window and aerogel insulation in the wall of a building. Experimental investigation of a 10 mm thick aerogel blanket surrounded box was conducted to assess its performance. Additionally, a CFD simulation was conducted, and the results of temperature degradation for the wall showed good agreement with experimental results. Additionally, the CFD simulation of temperature decay was compared between the aerogel-glazed window and argon-glazed window. It was found that the aerogel-glazed window has slower temperature decay compared to the argon-glazed window. The results showed that integrating aerogel in the glazing system and wall insulation in a building has the potential to reduce the building’s energy consumption. Moreover, a numeric simulation was conducted, and showed that the building’s annual energy consumption is reduced by 6% with the use of aerogel insulation compared to fiberglass.
Influence of Errors in Known Constants and Boundary Conditions on Solutions of Inverse Heat Conduction Problem
Authors: Kim Sun Kyoung
Published: June 2021

This work examines the effects of the known boundary conditions on the accuracy of the solution in one-dimensional inverse heat conduction problems. The failures in many applications of these problems are attributed to inaccuracy of the specified constants and boundary conditions. Since the boundary conditions and material properties in most thermal problems are imposed with uncertainty, the effects of their inaccuracy should be understood prior to the inverse analyses. The deviation from the exact solution has been examined for each case according to the errors in material properties, boundary location, and known boundary conditions. The results show that the effects of such errors are dramatic. Based on these results, the applicability and limitations of the inverse heat conduction analyses have been evaluated and discussed.
Perspective on the Response of Turbulent Pipe Flows to Strong Perturbations
Authors: Ding Liuyang , Van Buren Tyler , Gunady Ian E. , Smits Alexander J.
Published: June 2021

Pipe flow responds to strong perturbations in ways that are fundamentally different from the response exhibited by boundary layers undergoing a similar perturbation, primarily because of the confinement offered by the pipe wall, and the need to satisfy continuity. We review such differences by examining previous literature, with a particular focus on the response of pipe flow to three different kinds of disturbances: the abrupt change in surface condition from rough to smooth, the obstruction due to presence of a single square bar roughness elements of different sizes, and the flow downstream of a streamlined body-of-revolution placed on the centerline of the pipe. In each case, the initial response is strongly influenced by the pipe geometry, but far downstream all three flows display a common feature, which is the very slow, second-order recovery that can be explained using a model based on the Reynolds stress equations. Some future directions for research are also given.
Subdiffusive Reaction Model of Molecular Species in Liquid Layers: Fractional Reaction-Telegraph Approach
Authors: Tawfik Ashraf M. , Hefny Mohamed Mokhtar
Published: June 2021

In recent years, different experimental works with molecular simulation techniques have been developed to study the transport of plasma-generated reactive species in liquid layers. Here, we improve the classical transport model that describes the molecular species movement in liquid layers via considering the fractional reaction–telegraph equation. We have considered the fractional equation to describe a non-Brownian motion of molecular species in a liquid layer, which have different diffusivities. The analytical solution of the fractional reaction–telegraph equation, which is defined in terms of the Caputo fractional derivative, is obtained by using the Laplace–Fourier technique. The profiles of species density with the mean square displacement are discussed in each case for different values of the time-fractional order and relaxation time.
Spatial Characteristics of the Tourism Flows in China: A Study Based on the Baidu Index
Authors: Liu Yongwei , Liao Wang
Published: June 2021

The characteristics of information flow, as represented by the Baidu index, reflect the pattern of tourism flows between different cities. This paper is based on the Baidu index and applies the seasonal concentration index and social network analysis (SNA) methods to study the spatial structure characteristics of tourism flows in China. The results reveal that: (1) both the search volume of the Baidu index in different cities and the online attention to different scenic areas exhibit obvious spatial heterogeneity and seasonal differences; (2) regions with strong tourism flow connections mainly occur in the areas between metropolises or among the inner cities of urban agglomerations, which are largely distributed on the southeast side of the Heihe–Tengchong Line; (3) the development of the whole tourism flow network in China is low, with an unbalanced development between tourism supply and demand, indicating that tourism resources are concentrated in a few cities and that most of the information interaction among cities occurs in core areas, while a weak interaction is observed in peripheral areas; (4) cities like Beijing and Wuhan attain obvious advantages in regard to their tourism resources, whereas other cities, including Beijing, Shanghai, Shenzhen and Guangzhou, exhibit a high demand for tourism. Moreover, tourism information flow networks are concentrated in several cities with an important role in the Chinese urban system, such as Beijing, Wuhan, and Chengdu, because they contain abundant tourism resources, well-developed transportation systems and advanced economic and societal development levels. (5) Cities such as Beijing, Lhasa, Wuhan, and Zhengzhou possess numerous advantages due to structural holes, and they thus occur at an advantageous position in the tourism flow network.
3′ Tth Endonuclease Cleavage Polymerase Chain Reaction (3TEC-PCR) Technology for Single-Base-Specific Multiplex Pathogen Detection using a Two-Oligonucleotide System
Authors: Higgins Owen , Smith Terry
Published: June 2021

Polymerase chain reaction (PCR) is the standard in nucleic acid amplification technology for infectious disease pathogen detection and has been the primary diagnostic tool employed during the global COVID-19 pandemic. Various PCR technology adaptations, typically using two-oligonucleotide dye-binding methods or three-oligonucleotide hydrolysis probe systems, enable real-time multiplex target detection or single-base specificity for the identification of single-nucleotide polymorphisms (SNPs). A small number of two-oligonucleotide PCR systems facilitating both multiplex detection and SNP identification have been reported; however, these methods often have limitations in terms of target specificity, production of variable or false-positive results, and the requirement for extensive optimisation or post-amplification analysis. This study introduces 3′ Tth endonuclease cleavage PCR (3TEC-PCR), a two-oligonucleotide PCR system incorporating a modified primer/probe and a thermostable cleavage enzyme, Tth endonuclease IV, for real-time multiplex detection and SNP identification. Complete analytical specificity, low limits of detection, single-base specificity, and simultaneous multiple target detection have been demonstrated in this study using 3TEC-PCR to identify bacterial meningitis associated pathogens. This is the first report of a two-oligonucleotide, real-time multiplex PCR technology with single-base specificity using Tth endonuclease IV.
KCNK18 Biallelic Variants Associated with Intellectual Disability and Neurodevelopmental Disorders Alter TRESK Channel Activity
Authors: Pavinato Lisa , Nematian-Ardestani Ehsan , Zonta Andrea , De Rubeis Silvia , Buxbaum Joseph , Mancini Cecilia , Bruselles Alessandro , Tartaglia Marco , Pessia Mauro , Tucker Stephen J. , D’Adamo Maria Cristina , Brusco Alfredo
Published: June 2021

The TWIK-related spinal cord potassium channel (TRESK) is encoded by KCNK18, and variants in this gene have previously been associated with susceptibility to familial migraine with aura (MIM #613656). A single amino acid substitution in the same protein, p.Trp101Arg, has also been associated with intellectual disability (ID), opening the possibility that variants in this gene might be involved in different disorders. Here, we report the identification of KCNK18 biallelic missense variants (p.Tyr163Asp and p.Ser252Leu) in a family characterized by three siblings affected by mild-to-moderate ID, autism spectrum disorder (ASD) and other neurodevelopment-related features. Functional characterization of the variants alone or in combination showed impaired channel activity. Interestingly, Ser252 is an important regulatory site of TRESK, suggesting that alteration of this residue could lead to additive downstream effects. The functional relevance of these mutations and the observed co-segregation in all the affected members of the family expand the clinical variability associated with altered TRESK function and provide further insight into the relationship between altered function of this ion channel and human disease.
Loss of Individual Mitochondrial Ribonuclease P Complex Proteins Differentially Affects Mitochondrial tRNA Processing In Vivo
Authors: Saoji Maithili , Sen Aditya , Cox Rachel T.
Published: June 2021

Over a thousand nucleus-encoded mitochondrial proteins are imported from the cytoplasm; however, mitochondrial (mt) DNA encodes for a small number of critical proteins and the entire suite of mt:tRNAs responsible for translating these proteins. Mitochondrial RNase P (mtRNase P) is a three-protein complex responsible for cleaving and processing the 5′-end of mt:tRNAs. Mutations in any of the three proteins can cause mitochondrial disease, as well as mutations in mitochondrial DNA. Great strides have been made in understanding the enzymology of mtRNase P; however, how the loss of each protein causes mitochondrial dysfunction and abnormal mt:tRNA processing in vivo has not been examined in detail. Here, we used Drosophila genetics to selectively remove each member of the complex in order to assess their specific contributions to mt:tRNA cleavage. Using this powerful model, we find differential effects on cleavage depending on which complex member is lost and which mt:tRNA is being processed. These data revealed in vivo subtleties of mtRNase P function that could improve understanding of human diseases.
Hyperlipidemic Conditions Impact Force-Induced Inflammatory Response of Human Periodontal Ligament Fibroblasts Concomitantly Challenged with P. gingivalis-LPS
Authors: Symmank Judit , Appel Sophie , Bastian Jana Asisa , Knaup Isabel , Marciniak Jana , Hennig Christoph-Ludwig , Döding Annika , Schulze-Späte Ulrike , Jacobs Collin , Wolf Michael
Published: June 2021

In obese patients, enhanced serum levels of free fatty acids (FFA), such as palmitate (PA) or oleate (OA), are associated with an increase in systemic inflammatory markers. Bacterial infection during periodontal disease also promotes local and systemic low-grade inflammation. How both conditions concomitantly impact tooth movement is largely unknown. Thus, the aim of this study was to address the changes in cytokine expression and the secretion of human periodontal ligament fibroblasts (HPdLF) due to hyperlipidemic conditions, when additionally stressed by bacterial and mechanical stimuli. To investigate the impact of obesity-related hyperlipidemic FFA levels on HPdLF, cells were treated with 200 µM PA or OA prior to the application of 2 g/cm2 compressive force. To further determine the additive impact of bacterial infection, HPdLF were stimulated with lipopolysaccharides (LPS) obtained from Porphyromonas gingivalis. In mechanically compressed HPdLF, PA enhanced COX2 expression and PGE2 secretion. When mechanically stressed HPdLF were additionally stimulated with LPS, the PGE2 and IL6 secretion, as well as monocyte adhesion, were further increased in PA-treated cultures. Our data emphasize that a hyperlipidemic condition enhances the susceptibility of HPdLF to an excessive inflammatory response to compressive forces, when cells are concomitantly exposed to bacterial components.
Non-Coding RNAs in Kidney Diseases: The Long and Short of Them
Authors: Moreno Juan Antonio , Hamza Eya , Guerrero-Hue Melania , Rayego-Mateos Sandra , García-Caballero Cristina , Vallejo-Mudarra Mercedes , Metzinger Laurent , Metzinger-Le Meuth Valérie
Published: June 2021

Recent progress in genomic research has highlighted the genome to be much more transcribed than expected. The formerly so-called junk DNA encodes a miscellaneous group of largely unknown RNA transcripts, which contain the long non-coding RNAs (lncRNAs) family. lncRNAs are instrumental in gene regulation. Moreover, understanding their biological roles in the physiopathology of many diseases, including renal, is a new challenge. lncRNAs regulate the effects of microRNAs (miRNA) on mRNA expression. Understanding the complex crosstalk between lncRNA–miRNA–mRNA is one of the main challenges of modern molecular biology. This review aims to summarize the role of lncRNA on kidney diseases, the molecular mechanisms involved, and their function as emerging prognostic biomarkers for both acute and chronic kidney diseases. Finally, we will also outline new therapeutic opportunities to diminish renal injury by targeting lncRNA with antisense oligonucleotides.
Getting to the Heart of Left–Right Asymmetry: Contributions from the Zebrafish Model
Authors: Smith Kelly A. , Uribe Veronica
Published: June 2021

The heart is laterally asymmetric. Not only is it positioned on the left side of the body but the organ itself is asymmetric. This patterning occurs across scales: at the organism level, through left–right axis patterning; at the organ level, where the heart itself exhibits left–right asymmetry; at the cellular level, where gene expression, deposition of matrix and proteins and cell behaviour are asymmetric; and at the molecular level, with chirality of molecules. Defective left–right patterning has dire consequences on multiple organs; however, mortality and morbidity arising from disrupted laterality is usually attributed to complex cardiac defects, bringing into focus the particulars of left–right patterning of the heart. Laterality defects impact how the heart integrates and connects with neighbouring organs, but the anatomy of the heart is also affected because of its asymmetry. Genetic studies have demonstrated that cardiac asymmetry is influenced by left–right axis patterning and yet the heart also possesses intrinsic laterality, reinforcing the patterning of this organ. These inputs into cardiac patterning are established at the very onset of left–right patterning (formation of the left–right organiser) and continue through propagation of left–right signals across animal axes, asymmetric differentiation of the cardiac fields, lateralised tube formation and asymmetric looping morphogenesis. In this review, we will discuss how left–right asymmetry is established and how that influences subsequent asymmetric development of the early embryonic heart. In keeping with the theme of this issue, we will focus on advancements made through studies using the zebrafish model and describe how its use has contributed considerable knowledge to our understanding of the patterning of the heart.
Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes
Authors: Dunière Lysiane , Esparteiro Damien , Lebbaoui Yacine , Ruiz Philippe , Bernard Mickael , Thomas Agnès , Durand Denys , Forano Evelyne , Chaucheyras-Durand Frédérique
Published: June 2021

Background: In ruminants, physiological and nutritional changes occur peripartum. We investigated if gastro-intestinal microbiota, rumen metabolism and antioxidant status were affected around parturition and what could be the impact of a daily supplementation of a live yeast additive in late gestating ewes. Methods: Rumen, feces and blood samples were collected from 2 groups of 14 ewes one month and a few days before parturition, and 2 weeks postpartum. Results: In the control ewes close to parturition, slight changes in the ruminal microbiota were observed, with a decrease in the concentration F. succinogenes and in the relative abundance of the Fibrobacteres phylum. Moreover, a decrease in the alpha-diversity of the bacterial community and a reduced relative abundance of the Fibrobacteres phylum were observed in their feces. Control ewes were prone to oxidative stress, as shown by an increase in malondialdehyde (MDA) concentration, a lower total antioxidant status, and higher glutathione peroxidase (GPx) activity in the blood. In the yeast supplemented ewes, most of the microbial changes observed in the control group were alleviated. An increase in GPx activity, and a significant decrease in MDA concentration were measured. Conclusions: The live yeast used in this study could stabilize gastro-intestinal microbiota and reduce oxidative stress close to parturition.