bluuebunny/crossref_metadata_2025 huggingface dataset:
The Diversity of Subterranean Terrestrial Arthropods in Resava Cave (Eastern Serbia)
Authors: Vesović Nikola , Deltshev Christo , Mitov Plamen , Antić Dragan , Stojanović Dalibor Z. , Stojanović Dejan V. , Stojanović Katarina , Božanić Milenka , Ignjatović-Ćupina Aleksandra , Ćurčić Srećko
Published: April 2024

The Balkan region is rich in limestone deposits, which have created one of the largest hotspots of subterranean biodiversity. This paper gives an overview of the diversity of subterranean terrestrial arthropods in Resava Cave in eastern Serbia. This cave is protected and has the status of a natural monument. At the same time, it is one of the most visited caves in Serbia and its surroundings. Our study comprises the results of three years of biospeleological investigations of the famous Serbian cave in combination with data from the few available literature sources on the arthropod fauna of the cave. The arthropod samples were collected both manually and with pitfall traps. A total of 107 arthropod species from the four major subphyla were registered in the cave: 66 species of Hexapoda, 27 species of Chelicerata, 11 species of Myriapoda and three species of Crustacea. For four troglobitic, 16 troglophilic and 87 trogloxenic species recorded in the cave, descriptions of their microhabitats and information on their distribution in the cave are given. Considering the medium size of Resava Cave and the lack of permanent water flow in the two main levels on the one hand, and the large number of arthropod species recorded on the other, the cave is relatively rich in hypogean terrestrial arthropod fauna compared to other caves in Serbia that have been biospeleologically studied so far.
Risk Factors for Immune Checkpoint Inhibitor-Induced Liver Injury and the Significance of Liver Biopsy
Authors: Kawano Miki , Yano Yoshihiko , Yamamoto Atsushi , Yasutomi Eiichiro , Inoue Yuta , Kitadai Jun , Yoshida Ryutaro , Matsuura Takanori , Shiomi Yuuki , Ueda Yoshihide , Kodama Yuzo
Published: April 2024

Immune checkpoint inhibitor (ICI)-induced liver injury (LI) is a common adverse event, but the clinical characteristics based on the classification of hepatocellular injury and cholestatic types are not fully evaluated. This study aims to analyze risk factors and histological findings in relation to the classification of ICI-induced LI. In total, 254 ICI-induced LI patients among 1086 treated with ICIs between September 2014 and March 2022 were classified according to the diagnostic criteria for drug-induced LI (DILI), and their risk factors and outcomes were evaluated. Kaplan–Meier analyses showed that overall survival in patients with hepatocellular-injury-type LI was significantly longer than others (p < 0.05). Regarding pre-treatment factors, the lymphocyte count was significantly higher in patients with ICI-induced LI, especially in hepatocellular-injury-type LI. Gamma glutamyl transferase (γGTP) and alkaline phosphatase (ALP) were also significantly lower in patients with ICI-induced LI (p < 0.05). Multivariate analyses revealed that malignant melanoma, high lymphocyte count, and low ALP levels were extracted as factors contributing to hepatocellular-injury-type LI. The histological findings among 37 patients diagnosed as ICI-induced LI via liver biopsy also revealed that the spotty/focal necrosis was significantly frequent in hepatocellular-injury-type LI, whereas ductular reactions were frequently observed in cholestatic-type LI. It is suggested that the histological inflammation pattern in patients with LI is closely correlated with the type of DILI.
Flow-S: A Field-Deployable Device with Minimal Hands-On Effort to Concentrate and Quantify Schistosoma Circulating Anodic Antigen (CAA) from Large Urine Volumes
Authors: de Jong Daniëlle , Carrell Cody , Maganga Jane K. , Mhango Loyce , Shigella Peter S. , Gill Maddy , Shogren Ryan , Mullins Brianna , Warrick Jay W. , Changalucha John M. , van Dam Govert J. , Pham Khanh , Downs Jennifer A. , Corstjens Paul L. A. M.
Published: April 2024

A laboratory-based lateral flow (LF) test that utilizes up-converting reporter particles (UCP) for ultrasensitive quantification of Schistosoma circulating anodic antigen (CAA) in urine is a well-accepted test to identify active infection. However, this UCP-LF CAA test requires sample pre-treatment steps not compatible with field applications. Flow, a new low-cost disposable, allows integration of large-volume pre-concentration of urine analytes and LF detection into a single field-deployable device. We assessed a prototype Flow-Schistosoma (Flow-S) device with an integrated UCP-LF CAA test strip, omitting all laboratory-based steps, to enable diagnosis of active Schistosoma infection in the field using urine. Flow-S is designed for large-volume (5–20 mL) urine, applying passive paper-based filtration and antibody-based CAA concentration. Samples tested for schistosome infection were collected from women of reproductive age living in a Tanzania region where S. haematobium infection is endemic. Fifteen negative and fifteen positive urine samples, selected based on CAA levels quantified in paired serum, were analyzed with the prototype Flow-S. The current Flow-S prototype, with an analytical lower detection limit of 1 pg CAA/mL, produced results correlated with the laboratory-based UCP-LF CAA test. Urine precipitates occurred in frozen banked samples and affected accurate quantification; however, this should not occur in fresh urine. Based on the findings of this study, Flow-S appears suitable to replace the urine pre-treatment required for the laboratory-based UCP-LF CAA test, thus allowing true field-based applications with fresh urine samples. The urine precipitates observed with frozen samples, though less important given the goal of testing fresh urines, warrant additional investigation to evaluate methods for mitigation. Flow-S devices permit testing of pooled urine samples with applications for population stratified testing. A field test with fresh urine samples, a further optimized Flow-S device, and larger statistical power has been scheduled.
Connecting Prescriptive Analytics with Student Success: Evaluating Institutional Promise and Planning
Authors: Manly Catherine A.
Published: April 2024

Data-driven educational decisions enabled by online technologies hold promise for improving student performance across the full range of student dis/ability, even when efforts to design for student learning requirements (such as through Universal Design for Learning) fall short and undergraduates struggle to learn course material. In this action research study, 37 institutional stakeholders evaluated the potential of prescriptive analytics to project student outcomes in different simulated worlds, comparing hypothetical future learning scenarios. The goal of these prescriptions would be to make recommendations to students about tutoring and to faculty about beneficial course redesign points. The study’s analysis focused on the alignment of resources, processes, and values for feasible institutionalization of such analytics, highlighting institutional core values. In the postpandemic mix of online and on-campus learning under increasingly constrained resources, educational leaders should explore the potential competitive advantage of leveraging data from online technologies for greater student success.
Exploring the Complexity of Adaptive Teaching Expertise within Knowledge Generation Environments
Authors: Suh Jee Kyung , Hand Brian , Ercan-Dursun Jale , Sahin Ercin , Fulmer Gavin
Published: April 2024

The shift towards Next Generation Science Standards represents a paradigmatic change in teaching, transitioning from knowledge transmission to knowledge generation approaches. This reform underscores the complexity of teaching expertise, extending beyond mere knowledge to require a profound comprehension of generative learning environments. In this study, we explore Adaptive Teaching Expertise (AdTex), defining it as a teacher’s capacity characterized by fluidity and reflexiveness in teaching dynamics, rather than just flexibility. Through a complexity framing approach, we delineate three layers of AdTex: the visible actions of teachers, the semi-visible use of epistemic tools such as language, dialogue, and argument, and the tacit orientations towards learning that encompass epistemological, ontological, and axiological dimensions. Our research primarily investigates the intricate relationship between the epistemic tool and orientation layers. Our findings highlight the significance of an interconnected understanding and the impact of philosophical orientations on adaptive teaching practices. A notable contribution of this study is the development of a framework that articulates the belief and knowledge systems crucial for fostering generative learning environments, alongside the introduction of complexity maps to illustrate the interplay among these subsystems.
Chance-Constrained Optimal Design of PV-Based Microgrids under Grid Blackout Uncertainties
Authors: Alramlawi Mansour , Li Pu
Published: April 2024

A grid blackout is an intractable problem with serious economic consequences in many developing countries. Although it has been proven that microgrids (MGs) are capable of solving this problem, the uncertainties regarding when and for how long blackouts occur lead to extreme difficulties in the design and operation of the related MGs. This paper addresses the optimal design problem of the MGs considering the uncertainties of the blackout starting time and duration utilizing the kernel density estimator method. Additionally, uncertainties in solar irradiance and ambient temperature are also considered. For that, chance-constrained optimization is employed to design residential and industrial PV-based MGs. The proposed approach aims to minimize the expected value of the levelized cost of energy (LCOE), where the restriction of the annual total loss of power supply (TLPS) is addressed as a chance constraint. The results show that blackout uncertainties have a considerable effect on calculating the size of the MG’s components, especially the battery bank size. Additionally, it is proven that considering the uncertainties of the input parameters leads to an accurate estimation for the LCOE and increases the MG reliability level.
Radiated Electromagnetic Emission from Photovoltaic Systems—Measurement Results: Inverters and Modules
Authors: Kroner Désirée , Lundgren Urban , Augusto André , Bollen Math
Published: April 2024

Radiated electromagnetic emission of photovoltaic systems, for example, adversely impacting radiocommunication, can pose a major barrier against further increase in photovoltaic penetration. This is particularly the case near sensitive infrastructure and activities such as hospitals, airports, search and rescue, and military. To understand the impact of each component and installation detail, we performed systematic radiated electromagnetic emission measurements on comparable commercial photovoltaic systems in the frequency range 150 kHz to 30 MHz. Our measurements indicate that string inverters are unlikely to interfere with radiocommunication when installed according to recommended standards, rules, guidelines, and regulations. It was shown that module-level power optimizers are the main cause of high levels of radiated emissions. The frameless bifacial module showed higher levels of radiated emissions than the monofacial module with frame. Changes in cable management and earthing have less impact on radiated emissions than the choice of solar inverter concept and module type.
Comprehensive Assessment of Polysaccharides Extracted from Squash by Subcritical Water under Different Conditions
Authors: Zhang Yu , Sun Xun , Yang Bingjie , Li Fei , Yu Guoyong , Zhao Jing , Li Quanhong
Published: April 2024

The effects of subcritical water microenvironment on the physiochemical properties, antioxidant activity and in vitro digestion of polysaccharides (SWESPs) from squash were investigated. After single-factor experiments, twenty samples were successfully prepared at different extraction temperatures (110, 130, 150, 170 and 190 °C) and extraction times (4, 8, 12 and 16 min). Under a low temperature environment, the whole process was mainly based on the extraction of SWESP. At this time, the color of SWESP was white or light gray and the molecular mass was high. When the temperature was 150 °C, since the extraction and degradation of SWESP reached equilibrium, the maximum extraction rate (18.67%) was reached at 150 °C (12 min). Compared with traditional methods, the yield of squash SWESP extracted by subcritical water was 3–4 times higher and less time consuming. Under high temperature conditions, SWESPs were degraded and their antioxidant capacity and viscosity were reduced. Meanwhile, Maillard and caramelization reactions turned the SWESPs yellow-brown and produced harmful substances. In addition, different SWESPs had different effects on in vitro digestion. In brief, SWESPs prepared under different conditions have different structures and physicochemical properties, allowing the obtainment of the required polysaccharide. Our results show that squash polysaccharides prepared in different subcritical water states had good development potential and application in the food industry.
Prevention of Musculoskeletal Injuries in Gastrointestinal Endoscopists
Authors: Durič Tadej , Cibulkova Ivana , Hajer Jan
Published: April 2024

Gastroenterologists are exposed daily to musculoskeletal (MSK) stress during upper and lower gastrointestinal endoscopy, both during routine endoscopies and during long, demanding therapeutic procedures. There is evidence that endoscopy-related MSK injuries are becoming more common, particularly in the back, neck, shoulders, elbows, and hands. The aims of this study were to identify the most stressed muscle groups during endoscopy; to measure their activity using surface electromyographical (EMG) sensors; to detect areas of muscle overload; and to identify the number of microbreaks taken in specific muscles. Furthermore, we measured differences in the loading of these muscle groups with and without the use of special support systems such as a belt-like holder. Measurements were performed on 15 subjects (7 experienced endoscopists and 8 non-endoscopists). Due to this small sample size, inside each group, we achieved inconclusive results regarding statistically significant differences in different muscle groups. We increased the sample size by comparing all participants with and without the belt support system, disregarding their endoscopic background. There was a statistically significant difference (p < 0.05) in muscle tension and in levels of microbreaks in the muscles of the left forearm, biceps, and trapezius muscles. No statistically significant difference was observed in the muscle tension and level of microbreaks in the left deltoid muscle (p > 0.05). We hypothesize that the increased level of muscle loading and decreased level of microbreaks in the deltoid muscle are due to different muscle activity and different shoulder movements. Additionally, the deltoid muscle is not connected to the kinetic chain of body posture and stabilization. It is our belief that MSK injuries in gastrointestinal (GI) endoscopy can be prevented with the use of a belt-like support system.
Description of Phenological Events of Persian Walnut (Juglans regia L.) according to the Extended BBCH Scale and Historical Scales
Authors: Robin Julie , Bernard Anthony , Albouy Lisa , Papillon Sibylle , Tranchand Eloise , Hebrard Marie-Neige , Philibert Jean-Baptiste , Barbedette Marine , Schafleitner Sadia , Wenden Bénédicte , Barreneche Teresa , Delmas Marine , Lheureux Fabrice , Toillon Julien
Published: April 2024

Walnut trees are grown worldwide for their edible fruits, which have high nutritional value. To address climate change, researchers have studied walnut phenology to create cultivars adapted to warmer climates. The objective of this study is to propose a scale for phenological Persian walnut observations using the Biologische Bundesanstalt, Bundessortenamt, und CHemische Industrie (BBCH) codification and alignment with historical alphameric scales. Here, the principal growth stages (PGSs) of Persian walnut (Juglans regia L.) are described using stages from a previously available alphanumeric scale. This standardised phenological scale describes Persian walnut growth from the dormant vegetative state through reproductive budding and senescence. This phenological scale is expected to increase the efficiency of walnut phenological monitoring. Fifty-seven stages are used to describe the life cycle of Persian walnut in this BBCH scale. Of these 57 stages, 3 stages are dedicated to seed germination (PGS-0), 4 stages are dedicated to bud development (PGS-0), 7 stages are dedicated to leaf development (PGS-1), 4 stages are dedicated to stem elongation (PGS-3), 8 stages are dedicated to inflorescence emergence (PGS-5), 5 stages are dedicated to male flowering (PGS-6), 5 stages are dedicated to female flowering (PGS-6), 5 stages are dedicated to fruit development (PGS-7), 12 stages are dedicated to fruit ripening (PGS-8), and 4 stages are dedicated to leaf senescence (PGS-9).
Interventions to Foster Resilience in Family Caregivers of People with Alzheimer’s Disease: A Scoping Review
Authors: Santonja-Ayuso Lucía , Corchón-Arreche Silvia , Portillo Mari Carmen
Published: April 2024

The family caregiver of a person with Alzheimer’s disease still experiences, in most cases, negative consequences in their biopsychosocial environment, which are related to the acquisition of this role. However, it has been observed that this fact is not universal in this type of population since benefits can be obtained in the act of caring through the development of resilience. Given this possibility and given that nurses are the health professionals who support people in this illness process, there is an urgent need to identify which non-pharmacological interventions could improve or promote resilience in family caregivers of people with Alzheimer’s disease. Therefore, our overall objective was to determine which interventions are useful in promoting resilience in family caregivers of people with Alzheimer’s disease through a scoping review. The data were analysed using an adapted version of Arksey and O’Malley’s methodological framework, after critically reading the articles with the CasP and MMAT tools. Nine articles were included (five analytical experimental, two quantitative and two mixed). Three types of interventions related to promoting resilience in family caregivers of people with Alzheimer’s disease were identified: meditation, multicomponent psychoeducation and creative art; nurses participated as co-therapists in the last two.
Independent Correlates of Glycemic Control among Adults with Diabetes in South Africa
Authors: Hamid Abdulaziz , Dawson Aprill Z. , Xu Yilin , Egede Leonard E.
Published: April 2024

Background: Globally, the prevalence of diabetes is increasing, especially in low- and middle-income countries (LMICs), including those in the sub-Saharan African region. However, the independent socioeconomic correlates of glycemic control as measured by hemoglobin A1C have yet to be identified. Therefore, the aim of this analysis was to understand the independent correlates of glycemic control in South Africa. Methods: Data from the 2016 South Africa Demographic and Health Survey on adults with diabetes were used for this analysis. The dependent variable, glycemic control, was defined using hemoglobin A1c (HbA1c). Independent variables included: age, gender, ethnicity, marital status, region, urban/rural residence, ability to read, education, insurance, wealth, occupation, and employment in the last year. Analysis of variance was used to test for differences in mean HbA1c for each category of all independent variables, and a fully adjusted linear regression model was used to identify independent correlates of glycemic control (HbA1c). Results: Among the 772 people included in this analysis, there were significant differences in mean HbA1c by age (p < 0.001), ethnicity (p < 0.001), place of residence (p = 0.024), wealth index (p = 0.001), and employment in the last year (p = 0.008). Independent correlates of HbA1c included age, ethnicity, and wealth index. Conclusions: This study used data from a large diverse population with a high prevalence of diabetes in sub-Saharan Africa and provides new evidence on the correlates of glycemic control and potential targets for interventions designed to lower HbA1c and improve diabetes-related health outcomes of adults in South Africa.
Effects of Reduced Extracellular Sodium Concentrations on Cisplatin Treatment in Human Tumor Cells: The Role of Autophagy
Authors: Naldi Laura , Fibbi Benedetta , Anceschi Cecilia , Nardini Patrizia , Guasti Daniele , Peri Alessandro , Marroncini Giada
Published: April 2024

Hyponatremia is the prevalent electrolyte imbalance in cancer patients, and it is associated with a worse outcome. Notably, emerging clinical evidence suggests that hyponatremia adversely influences the response to anticancer treatments. Therefore, this study aims to investigate how reduced extracellular [Na+] affects the responsiveness of different cancer cell lines (from human colon adenocarcinoma, neuroblastoma, and small cell lung cancer) to cisplatin and the underlying potential mechanisms. Cisplatin dose–response curves revealed higher IC50 in low [Na+] than normal [Na+]. Accordingly, cisplatin treatment was less effective in counteracting the proliferation and migration of tumor cells when cultured in low [Na+], as demonstrated by colony formation and invasion assays. In addition, the expression analysis of proteins involved in autophagosome–lysosome formation and the visualization of lysosomal areas by electron microscopy revealed that one of the main mechanisms involved in chemoresistance to cisplatin is the promotion of autophagy. In conclusion, our data first demonstrate that the antitumoral effect of cisplatin is markedly reduced in low [Na+] and that autophagy is an important mechanism of drug escape. This study indicates the role of hyponatremia in cisplatin chemoresistance and reinforces the recommendation to correct this electrolyte alteration in cancer patients.
A Multi-Faceted Analysis Showing CRNDE Transcripts and a Recently Confirmed Micropeptide as Important Players in Ovarian Carcinogenesis
Authors: Balcerak Anna , Szafron Laura Aleksandra , Rubel Tymon , Swiderska Bianka , Bonna Arkadiusz M. , Konarzewska Magdalena , Sołtyszewski Ireneusz , Kupryjanczyk Jolanta , Szafron Lukasz Michal
Published: April 2024

CRNDE is considered an oncogene expressed as long non-coding RNA. Our previous paper is the only one reporting CRNDE as a micropeptide-coding gene. The amino acid sequence of this micropeptide (CRNDEP) has recently been confirmed by other researchers. This study aimed at providing a mass spectrometry (MS)-based validation of the CRNDEP sequence and an investigation of how the differential expression of CRNDE(P) influences the metabolism and chemoresistance of ovarian cancer (OvCa) cells. We also assessed cellular localization changes of CRNDEP, looked for its protein partners, and bioinformatically evaluated its RNA-binding capacities. Herein, we detected most of the CRNDEP sequence by MS. Moreover, our results corroborated the oncogenic role of CRNDE, portraying it as the gene impacting carcinogenesis at the stages of DNA transcription and replication, affecting the RNA metabolism, and stimulating the cell cycle progression and proliferation, with CRNDEP being detected in the centrosomes of dividing cells. We also showed that CRNDEP is located in nucleoli and revealed interactions of this micropeptide with p54, an RNA helicase. Additionally, we proved that high CRNDE(P) expression increases the resistance of OvCa cells to treatment with microtubule-targeted cytostatics. Furthermore, altered CRNDE(P) expression affected the activity of the microtubular cytoskeleton and the formation of focal adhesion plaques. Finally, according to our in silico analyses, CRNDEP is likely capable of RNA binding. All these results contribute to a better understanding of the CRNDE(P) role in OvCa biology, which may potentially improve the screening, diagnosis, and treatment of this disease.
Insight into the Interplay of Gd-IgA1, HMGB1, RAGE and PCDH1 in IgA Vasculitis (IgAV)
Authors: Held Martina , Kozmar Ana , Sestan Mario , Turudic Daniel , Kifer Nastasia , Srsen Sasa , Gagro Alenka , Frkovic Marijan , Jelusic Marija
Published: April 2024

The pathogenesis of IgAV, the most common systemic vasculitis in childhood, appears to be complex and requires further elucidation. We aimed to investigate the potential role of galactose-deficient immunoglobulin A1 (Gd-IgA1), high-mobility group box 1 (HMGB1), receptor for advanced glycation end products (RAGE) and protocadherin 1 (PCDH1) in the pathogenesis of IgAV. Our prospective study enrolled 86 patients with IgAV and 70 controls. HMGB1, RAGE, Gd-IgA1 and PCDH1 in serum and urine were determined by the enzyme-linked immunosorbent assay (ELISA) method at the onset of the disease and after a six-month interval in patients and once in the control group. Serum concentrations of HMGB1, RAGE and PCDH1 and urinary concentrations of HMGB1, RAGE, Gd-IgA1 and PCDH1 were significantly higher in patients with IgAV than in the control group (p < 0.001). Concentrations of HMGB1 (5573 pg/mL vs. 3477 pg/mL vs. 1088 pg/mL, p < 0.001) and RAGE (309 pg/mL vs. 302.4 pg/mL vs. 201.3 pg/mL, p = 0.012) in the serum of patients remained significantly elevated when the disease onset was compared with the six-month follow-up interval, and thus could be a potential marker of disease activity. Urinary concentration of HMGB1 measured in the follow-up period was higher in patients with nephritis compared to IgAV without nephritis (270.9 (146.7–542.7) ng/mmol vs. 133.2 (85.9–318.6) ng/mmol, p = 0.049) and significantly positively correlated with the urine albumine to creatinine ratio (τ = 0.184, p < 0.05), the number of erythrocytes in urine samples (τ = 0.193, p < 0.05) and with the outcome of nephritis (τ = 0.287, p < 0.05); therefore, HMGB1 could be a potential tool for monitoring patients with IgAV who develop nephritis. Taken together, our results imply a possible interplay of Gd-IgA1, HMGB1, RAGE and PCDH1 in the development of IgAV. The identification of sensitive biomarkers in IgAV may provide disease prevention and future therapeutics.
Targeted Clindamycin Delivery Systems: Promising Options for Preventing and Treating Bacterial Infections Using Biomaterials
Authors: Słota Dagmara , Jampilek Josef , Sobczak-Kupiec Agnieszka
Published: April 2024

Targeted therapy represents a real opportunity to improve the health and lives of patients. Developments in this field are confirmed by the fact that the global market for drug carriers was worth nearly $40 million in 2022. For this reason, materials engineering and the development of new drug carrier compositions for targeted therapy has become a key area of research in pharmaceutical drug delivery in recent years. Ceramics, polymers, and metals, as well as composites, are of great interest, as when they are appropriately processed or combined with each other, it is possible to obtain biomaterials for hard tissues, soft tissues, and skin applications. After appropriate modification, these materials can release the drug directly at the site requiring a therapeutic effect. This brief literature review characterizes routes of drug delivery into the body and discusses biomaterials from different groups, options for their modification with clindamycin, an antibiotic used for infections caused by aerobic and anaerobic Gram-positive bacteria, and different methods for the final processing of carriers. Examples of coating materials for skin wound healing, acne therapy, and bone tissue fillers are given. Furthermore, the reasons why the use of antibiotic therapy is crucial for a smooth and successful recovery and the risks of bacterial infections are explained. It was demonstrated that there is no single proven delivery scheme, and that the drug can be successfully released from different carriers depending on the destination.
Future Climate Change and Anthropogenic Disturbance Promote the Invasions of the World’s Worst Invasive Insect Pests
Authors: Cao Runyao , Feng Jianmeng
Published: April 2024

Invasive insect pests adversely impact human welfare and global ecosystems. However, no studies have used a unified scheme to compare the range dynamics of the world’s worst invasive insect pests. We investigated the future range shifts of 15 of the world’s worst invasive insect pests. Although future range dynamics varied substantially among the 15 worst invasive insect pests, most exhibited large range expansions. Increases in the total habitat suitability occurred in more than ca. 85% of global terrestrial regions. The relative impacts of anthropogenic disturbance and climate variables on the range dynamics depended on the species and spatial scale. Aedes albopictus, Cinara cupressi, and Trogoderma granarium occurred four times in the top five largest potential ranges under four future climate scenarios. Anoplophora glabripennis, Aedes albopictus, and Co. formosanus were predicted to have the largest range expansions. An. glabripennis, Pl. manokwari, Co. formosanus, and So. invicta showed the largest range centroid shifts. More effective strategies will be required to prevent their range expansions. Although the strategies should be species-specific, mitigating anthropogenic disturbances and climate change will be essential to preventing future invasions. This study provides critical and novel insights for developing global strategies to combat the invasions of invasive insect pests in the future.
A Randomized Controlled Trial Comparing Collagen Matrix to Hemostatic Gelatin Sponge as Socket Seal in Alveolar Ridge Preservation
Authors: Eeckhout Célien , Seyssens Lorenz , Glibert Maarten , Keppens Laurens , Nollet Baptist , Lambert Martijn , Cosyn Jan
Published: April 2024

(1) Objectives: This study aimed to compare a collagen matrix to a hemostatic gelatin sponge as a socket seal in alveolar ridge preservation (ARP). (2) Methods: Systemically healthy patients planned for ARP at two sites with more than 50% of the buccal bone wall remaining after tooth extraction were eligible for inclusion. ARP involved socket grafting using collagen-enriched deproteinized bovine bone mineral. Sites were then randomly assigned to the test group (collagen matrix) or the control group (hemostatic gelatin sponge). The primary outcome was soft tissue thickness in the center of the site at 4 months, analyzed on cone-beam computed tomography. Secondary outcomes included the buccal and lingual soft tissue heights, horizontal bone loss, buccal soft tissue profile changes, wound dimensions, and Socket Wound Healing Score (SWHS). (3) Results: In total, 18 patients (12 females, 6 males) with a mean age of 57.3 years (SD 11.1) were included. Four months after ARP, the soft tissue thickness in the center of the site amounted to 2.48 mm (SD 0.70) in the test group and 1.81 mm (SD 0.69) in the control group. The difference of 0.67 mm (95% CI: 0.20–1.14) in favor of the collagen matrix was statistically significant (p < 0.009). The buccal soft tissue height was also statistically significantly higher for the collagen matrix (0.72 mm; 95% CI: 0.06–1.38; p = 0.034). A trend favoring the collagen matrix was found for the lingual soft tissue height (p = 0.066). No significant differences between the groups in terms of horizontal bone loss, buccal soft tissue profile changes, wound dimensions, and the SWHS were found. (4) Conclusions: The absence of significant differences in hard tissue outcomes suggests that both the collagen matrix and hemostatic gelatin sponge effectively sealed the extraction socket and supported bone preservation. However, the collagen matrix better maintained soft tissue dimensions. The clinical relevance of this finding with respect to the necessity for adjunctive soft tissue augmentation at the time of implant placement is yet to be studied.
Dysregulation of Inflammatory Pathways in Adult Spinal Deformity Patients with Frailty
Authors: Tabata Tomohisa , Yagi Mitsuru , Suzuki Satoshi , Takahashi Yohei , Ozaki Masahiro , Tsuji Osahiko , Nagoshi Narihito , Matsumoto Morio , Nakamura Masaya , Watanabe Kota
Published: April 2024

Background/Objectives: An important aspect of the pathophysiology of frailty seems to be the dysregulation of inflammatory pathways and the coagulation system. However, an objective assessment of the impact of frailty on the recovery from surgery is not fully studied. This study sought to assess how frailty affects the recovery of adult spinal deformity (ASD) surgery using blood biomarkers. Methods: 153 consecutive ASD patients (age 64 ± 10 yr, 93% female) who had corrective spine surgery in a single institution and reached 2y f/u were included. The subjects were stratified by frailty using the modified frailty index-11 (robust [R] group or prefrail and frail [F] group). Results of commonly employed laboratory tests at baseline, 1, 3, 7, and 14 post-operative days (POD) were compared. Further comparison was performed in propensity-score matched-39 paired patients between the groups by age, curve type, and baseline alignment. A correlation between HRQOLs, major complications, and biomarkers was performed. Results: Among the propensity-score matched groups, CRP was significantly elevated in the F group at POD1,3(POD1; 5.3 ± 3.1 vs. 7.9 ± 4.7 p = 0.02, POD3; 6.6 ± 4.6 vs. 8.9 ± 5.2 p = 0.02). Transaminase was also elevated in the F group at POD3(ASD: 36 ± 15 vs. 51 ± 58 U/L, p = 0.03, ALT: 32 ± 16 vs. 47 ± 55 U/L, p = 0.04). Interestingly, moderate correlation was observed between transaminase at POD1 and 2 y SRS22 (AST; function r = −0.37, mental health r = −0.39, satisfaction −0.28, total r = −0.40, ALT; function r = −0.37, satisfaction −0.34, total r = −0.39). Conclusions: Frailty affected the serum CRP and transaminase differently following ASD surgery. Transaminase at early POD was correlated with 2 y HRQOLs. These findings support the hypothesis that there is a specific physiological basis to the frailty that is characterized in part by increased inflammation and that these physiological differences persist.
Validation of the Novel Web-Based Application HUMTELEMED for a Comprehensive Assessment of Cardiovascular Risk Based on the 2021 European Society of Cardiology Guidelines
Authors: Landolfo Matteo , Spannella Francesco , Gezzi Alessandro , Giulietti Federico , Sabbatini Lucia , Bari Isabella , Alessandroni Romina , Di Agostini Angelica , Turri Paolo , Alborino Francesco , Scoppolini Massini Lorenzo , Sarzani Riccardo
Published: April 2024

Background and aims: SCORE2/SCORE2-OP cardiovascular risk (CVR) charts and online calculators do not apply to patients with comorbidities, target organ damage, or atherosclerotic cardiovascular disease, for whom the assessment relies on the conventional consultation of the 2021 ESC guidelines (qualitative approach). To simplify the CVR evaluation, we developed an integrated multi-language and free-to-use web application. This study assessed the agreement between the conventional method versus our web app. Methods: A cross-sectional study was carried out on 1306 consecutive patients aged 40+ years referred to our center for the diagnosis and management of hypertension and dyslipidemia. Two double-blind operators performed the CVR assessment and classified each patient into low–moderate-, high-, and very-high-risk categories by using the conventional method (SCORE2/SCORE2-OP charts and consultation of the 2021 ESC guidelines) and the web app. The Kappa statistics were used to compare the two methods. Results: The mean age was 60.3 ± 11.9 years, with male prevalence (51.4%). Patients in primary prevention were 77.0%. According to the SCORE2/SCORE2-OP charts and 2021 ESC guideline consultation, the CVR was low–moderate in 18.6% (n° 243), high in 36.8% (n° 480), and very high in 44.6% (n° 583). According to the web app, individual CVR was low–moderate in 19.5% (n° 255), high in 35.4% (n° 462), and very high in 45.1% (n° 589). The two methods strongly agreed (Kappa = 0.960, p < 0.001), with a 97.5% concordance. Conclusions: our application has excellent reliability in a broad “real life” population and may help non-expert users and busy clinicians to assess individual CVR appropriately, representing a free-to-use, simple, time-sparing and widely available alternative to the conventional CVR evaluation using SCORE2/SCORE2-OP and 2021 ESC guideline charts.
Resistance Training to Muscle Failure with Variable Load Intensities: Implications for Post-Exercise Blood Pressure and Heart Rate Variability in Trained Men
Authors: Güngör Ali Kamil , Topçu Hüseyin , Aldhahi Monira I. , Al-Mhanna Sameer Badri , Gülü Mehmet
Published: April 2024

Background: The modulation of cardiac sympathovagal balance alters following acute resistance training (RT) sessions. Nevertheless, the precise influence of RT at varying load intensities on this physiological response remains to be fully elucidated. Therefore, the aim of this study was to compare the time course of recovery following low- (40%), moderate- (60%), and high- (80%) load-intensity RT protocols performed up to muscle repetition failure in resistance-trained men. Method: Sixteen young, resistance-trained men (mean age: 21.6 ± 2.5 years, mean height: 175.7 ± 8.9 cm, mean weight: 77.1 ± 11.3 kg) participated in a randomized crossover experimental design involving three sessions, each taken to the point of muscle failure. These sessions were characterized by different load intensities: low (40% of 1-repetition maximum, 1RM), moderate (60% of 1RM), and high (80% of 1RM). The exercise regimen comprised four exercises—back squat (BS), bench press (BnP), barbell row (BR), and shoulder press (SP)—with each exercise consisting of three sets. Throughout each session, heart rate variability (HRV) and blood pressure (BP) parameters were assessed both pre-exercise and during a 40 min post-exercise period, segmented into 10 min intervals for stabilization. Statistical analysis involved the use of a repeated measures ANOVA. Results: It was observed that the 40% and 60% RT sessions resulted in a significantly higher root mean square of successive R-R intervals (RMSSD) value compared to the 80% RT session in the post-exercise recovery process in 30 min (respectively, p = 0.025; p = 0.028) and 40 min (respectively, p = 0.031; p = 0.046), while the 40% and 60% RT sessions produced similar responses. The 40% RT session was significantly higher in the high frequency (HF) value post-exercise in 40 min compared to the 80% RT session (p = 0.045). Conclusions: Our findings suggest that engaging in resistance training (RT) sessions to muscle failure at an intensity of 80% induces acute increases in sympathetic activity, potentially leading to elevated cardiovascular stress. For individuals with normal blood pressure, it is advisable to opt for lighter loads and higher repetition volumes when prescribing RT, as heavier-load RT may carry an increased risk of cardiac-related factors.
Management of a Rare Case of Multiple Coronary Artery Fistulas Associated with Ascending Aortic and Root Aneurysm: Case Report and Review of Literature
Authors: Robu Mircea , Radulescu Bogdan , Nayyerani Reza , Enache Robert , Stiru Ovidiu , Iosifescu Andrei , Olaru Georgiana , Ciomag (Ianula) Raluca , Iliescu Vlad Anton , Moldovan Horatiu
Published: April 2024

Coronary artery fistulas draining into the left ventricle is a rare finding. They can be associated with other congenital cardiac anomalies like ventricular septal defect or tetralogy of Fallot. While most of them are asymptomatic, they can lead to severe cardiac complications like infective endocarditis, heart failure, or myocardial ischemia. Symptomatic coronary artery fistulas can be managed surgically or percutaneously. We present a case of a 61-year-old male patient with both left anterior descending artery and right coronary artery fistulas draining into the left ventricle associated with ascending aorta and root aneurysm. Preoperative assessment for myocardial ischemia and the size and location of the fistulas was performed. The echocardiography stress test was negative. Surgery consisted of replacement of the ascending aorta and reconstruction of the noncoronary sinus with a Dacron patch with aortic valve preservation and no intervention for the coronary artery fistulas. The surgical strategy was adapted for cardioplegia administration to compensate for the volume of coronary blood drained into the left ventricle and for better protection of the distal myocardium.
Exploring the Effects of Graphene-Based Nanoparticles on Early Salmonids Cardiorespiratory Responses, Swimming and Nesting Behavior
Authors: Makaras Tomas , Jakubowska-Lehrmann Magdalena , Jurgelėnė Živilė , Šemčuk Sergej
Published: April 2024

Graphene-based nanomaterials are exceptionally attractive for a wide range of applications, raising the likelihood of the release of graphene-containing nanoparticles into aquatic environments. The growing use of these carbon nanomaterials in different industries highlights the crucial need to investigate their environmental impact and evaluate potential risks to living organisms. The current investigation evaluated the nanotoxicity of graphene (nanoflakes) and graphene oxide (GO) nanoparticles on the cardiorespiratory responses (heart rate, gill ventilation frequency), as well as the swimming and nesting behavioral parameters of early stage larvae and juvenile salmonids. Both short-term (96 h) and long-term (23 days) exposure experiments were conducted using two common species: brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss). The findings demonstrated notable alterations in fish nesting behavior, swimming performance, and cardiorespiratory functions, indicating the potential toxicity of nanoparticles. This impact was observed at both physiological and whole-organismal levels in salmonids at early stages. Future investigations should explore different types of nanocarbons and their potential enduring effects on fish population structure, considering not only individual survival but also broader aspects of development, including feeding, reproductive, and other social dynamics.
Navigating the Terrain of Digital Transition: Ghana’s Journey of Developing a Digital Land Information System
Authors: Owusu Ansah Rosemond , Abubakari Zaid , Quaye Benjamin , Todorovski Dimo , Malumbo Chipofya , Lemmen Christiaan
Published: April 2024

This paper explores Ghana’s two-decade journey in developing a digital land information system (LIS), exemplified by Ghana Enterprise Land Information System (GELIS) and Enterprise Land Information System (ELIS). Despite advancements by Ghana’s Lands Commission, manual processes persist and coexist with digital ones. Our study uses a qualitative approach to assess the effectiveness of the LIS implemented in Accra. This study found that Accra’s LIS is highly aligned with clear institutional mandates, roles, and responsibilities, as well as the availability of laws and policies to support analogue-to-digital conversion. Furthermore, a robust approach to protecting the data, operating system, and software underpins the system. Subsequently, the integration of a digital LIS has enhanced service delivery and accountability. Nonetheless, Accra’s LIS is still at the beginning of a comprehensive learning and development curve. Sustaining the LIS requires furtherance in the implementation plan, funding, law implementation, ICT strategy, divisional integration, work processes, data quality, and communication strategy. These findings will inform the next steps of improvement for Accra’s LIS and guide its nationwide scaling, contributing to discussions on technology acceptance dynamics in predominantly analogue environments.
Insights into the Novel Cardiac Biomarker in Acute Heart Failure: Mybp-C
Authors: Chetran Adriana , Bădescu Minerva Codruţa , Şerban Ionela Lăcrămioara , Duca Ştefania Teodora , Afrăsânie Irina , Cepoi Maria-Ruxandra , Dmour Bianca Ana , Matei Iulian Theodor , Haba Mihai Ştefan Cristian , Costache Alexandru Dan , Mitu Ovidiu , Cianga Corina Maria , Tuchiluş Cristina , Constantinescu Daniela , Costache-Enache Irina Iuliana
Published: April 2024

(1) Background: Given its high cardiac specificity and its capacity to directly assess the cardiac function, cardiac myosin-binding protein (MyBP-C) is a promising biomarker in patients with acute heart failure (AHF). The aim of our study was to investigate the clinical utility of this novel marker for diagnosis and short-term prognosis in subjects with AHF. (2) Methods: We measured plasma levels of MyBP-C at admission in 49 subjects (27 patients admitted with AHF and 22 controls). (3) Results: The plasma concentration of MyBP-C was significantly higher in patients with AHF compared to controls (54.88 vs. 0.01 ng/L, p < 0.001). For 30-day prognosis, MyBP-C showed significantly greater AUC (0.972, p < 0.001) than NT-proBNP (0.849, p = 0.001) and hs-TnI (0.714, p = 0.047). In a multivariate logistic regression analysis, an elevated level of MyBP-C was the best independent predictor of 30-day mortality (OR = 1.08, p = 0.039) or combined death/recurrent 30-days rehospitalization (OR = 1.12, p = 0.014). (4) Conclusions: Our data show that circulating MyBP-C is a sensitive and cardiac-specific biomarker with potential utility for the accurate diagnosis and prognosis of AHF.
Low-Temperature Synthesis of Bi2S3 Hierarchical Microstructures via Co-Precipitation and Digestive Process in Aqueous Medium
Authors: Carrasco-González José Alfonso , Ortega-Amaya Rebeca , Díaz-Torres Esteban , Pérez-Guzmán Manuel A. , Ortega-López Mauricio
Published: April 2024

Bismuth sulfide (Bi2S3) nanostructures have gained significant attention in the fields of catalysis, optoelectronics, and biomedicine due to their unique physicochemical properties. This paper introduces a simple and cost-effective method for producing Bi2S3 microstructures at low temperatures (25 and 70 °C). These microstructures are formed by the hierarchical self-assembly of Bi2S3 nanoparticles, which are typically 15–40 nm in size. The nanoparticles are synthesized by the co-precipitation of thioglycolic acid, thioacetamide, and bismuth nitrate in water. The study delves into the phase composition and morphological evolution of the microstructures, concerning the chemical composition of the solution and the synthesis temperature. X-ray analysis has confirmed the formation of single-phase bismuthinite Bi2S3. The synthesis process generates primary building blocks in the form of 15–40 nm Bi2S3 nanocrystals, which then go through a hierarchical self-assembly process to produce a range of micrometer-sized structures. A scanning electron microscopy examination revealed that the primary nanoparticles self-assemble into quasi-1D worm-like nanostructures, which then self-assemble to create sponge-shaped microstructures. These structures subsequently self-organize and refine into either flower- or dandelion-like microstructures, mostly depending on the synthesis temperature and the chemistry of the digestion medium.