bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Optimal Binders for Torrefied Biomass Pellets
Authors: Butler James W. , Skrivan William , Lotfi Samira
Published: April 2023

The pretreatment of biomass through torrefaction is an effective means of improving the fuel quality of woody biomass and its suitability for use in existing facilities burning thermal coal. Densification of torrefied biomass produces a fuel of similar energy density, moisture content, and fixed carbon content to low-grade coals. Additionally, if the torrefaction conditions are optimized, the produced torrefied pellet will be resistant to weathering and biological degradation, allowing for outdoor storage and transport in a manner similar to coal. In untreated biomass, lignin is the primary binding agent for biomass pellets and is activated by the heat and pressures of the pellet extrusion process. The thermal degradation of lignin during torrefaction reduces its binding ability, resulting in pellets of low durability not suitable for transportation. The use of a binding agent can increase the durability of torrefied pellets/briquettes through a number of different binding mechanisms depending on the binder used. This study gives a review of granular binding mechanisms, as they apply to torrefied biomass and assesses a variety of organic and inorganic binding agents, ranking them on their applicability to torrefied pellets based on a number of criteria, including durability, hydrophobicity, and cost. The best binders were found to be solid lignin by-product derived from pulp and paper processing, biomass tar derived from biomass pyrolysis, tall oil pitch, and lime.
Experimental Investigation of Thermal Runaway Behavior and Hazards of a 1440 Ah LiFePO4 Battery Pack
Authors: Chen Hao , Yang Kai , Liu Youwei , Zhang Mingjie , Liu Hao , Liu Jialiang , Qu Zhanzhan , Lai Yilin
Published: April 2023

The thermal runaway (TR) behavior and combustion hazards of lithium-ion battery (LIB) packs directly determine the implementation of firefighting and flame-retardants in energy storage systems. This work studied the TR propagation process and dangers of large-scale LIB packs by experimental methods. The LIB pack consisted of twenty-four 60 Ah (192 Wh) LIBs with LiFePO4 (LFP) as the cathode material. Flame performance, temperature, smoke production, heat release rate (HRR), and mass loss were analyzed during the experiment. The results indicated that TR propagation of the LIB pack developed from the outside to the inside and from the middle to both sides. The development process could be divided into five stages corresponding to the combustion HRR peaks. In the initial stages, the main factor causing LFP battery TR under heating conditions was the external heat source. With the propagation of TR, heat conduction between batteries became the main factor. Hazard analysis found that the HRRmax of the LIB pack was 314 KW, more than eight times that of a single 60 Ah battery under heating conditions. The LIB pack had higher normalized mass loss and normalized THR (6.94 g/Ah and 187 KJ/Ah, respectively) than a single LFP battery. This study provides a reference for developing strategies to address TR propagation or firefighting in energy storage systems.
Critical Review on Community-Shared Solar—Advantages, Challenges, and Future Directions
Authors: Narjabadifam Nima , Fouladvand Javanshir , Gül Mustafa
Published: April 2023

In the last few years, many innovative solutions have been presented to address the climate change crisis. One of the innovative solutions is the participation of community members in the collective production of solar electricity instead of individual production. The current study aims to provide a critical literature review of the collective production of solar electricity, which is called “community-shared solar” (CSS). Sixty-seven peer-reviewed publications were selected based on the setting up of a combination of related keywords. To analyze the concept of CSS in the existing literature, a multi-level perspective (MLP) framework was used to observe the CSS innovation at the niche, regime, and landscape levels. Four aspects, including the technical, economic, socio-political, and regulatory and institutional, were considered to evaluate those three levels. The results revealed that in the technical and economic aspects, CSS has reached maturity and internal momentum that can take it to the next levels. However, a lack of attention to the socio-political aspect and the regulatory and institutional aspect, in particular, is the potential barrier to the emergence of CSS and its potential position as a leading energy system.
Recent Trends in Accounting and Information System Research: A Literature Review Using Textual Analysis Tools
Authors: Albuquerque Fábio , Dos Santos Paula Gomes
Published: April 2023

Accounting has been evolving to follow the latest economic, political, social, and technological developments. Therefore, there is a need for researchers to also include in their research agenda the emerging topics in the accounting area. This exploratory paper selects technological matters in accounting as its research object, proposing a literature review that uses archival research as a method and content analysis as a technique. Using different tools for the assessment of qualitative data, this content analysis provides a summary of those papers, such as their main topics, most frequent words, and cluster analysis. A top journal was used as the source of information, namely The International Journal of Accounting Information Systems, given its scope, which links accounting and technological matters. Data from 2000 to 2022 was selected to provide an evolutive analysis since the beginning of this century, with a particular focus on the latest period. The findings indicate that the recent discussions and trending topics in accounting, including matters such as international regulation, the sustainable perspective in accounting, as well as new methods, channels, and processes for improving the entities’ auditing and reporting, have increased their relevance and influence, enriching the debate and future perspectives in combination with the use of new technologies. Therefore, this seems to be a path to follow as an avenue for future research. Notwithstanding, emerging technologies as a research topic seem to be slower or less evident than their apparent development in the accounting area. The findings from this paper are limited to a single journal and, therefore, this limitation must be considered in the context of those conclusions. Notwithstanding, its proposed analysis may contribute to the profession, academia, and the scientific community overall, enabling the identification of the state of the art of literature in the technological area of accounting.
Exploration of the Burning Question: A Long History of Fire in Eastern Australia with and without People
Authors: Constantine Mark , Williams Alan N. , Francke Alexander , Cadd Haidee , Forbes Matt , Cohen Tim J. , Zhu Xiaohong , Mooney Scott D.
Published: April 2023

Ethnographic observations suggest that Indigenous peoples employed a distinct regime of frequent, low-intensity fires in the Australian landscape in the past. However, the timing of this behaviour and its ecological impact remain uncertain. Here, we present detailed analysis of charcoal, including a novel measure of fire severity using Fourier transform infrared (FTIR) spectroscopy, at a site in eastern Australia that spans the last two glacial/interglacial transitions between 135–104 ka and 18–0.5 ka BP (broadly equivalent to Marine Isotope Stage (MIS) 6-5 and 2-1, respectively). The accumulation of charcoal and vegetation composition was similar across both periods, correlating closely with Antarctic ice core records, and suggesting that climate is the main driver of fire regimes. Fire severity was lower over the past 18,000 years compared to the penultimate glacial/interglacial period and suggests increasing anthropogenic influence over the landscape during this time. Together with local archaeological records, our data therefore imply that Indigenous peoples have been undertaking cultural burning since the beginning of the Holocene, and potentially the end of the Last Glacial Maximum. We highlight the fact that this signal is not easily discernible in the other proxies examined, including widely used charcoal techniques, and propose that any anthropogenic signal will be subtle in the palaeo-environmental record. While early Indigenous people’s reasons for landscape burning were different from those today, our findings nonetheless suggest that the current land management directions are based on a substantive history and could result in a reduction in extreme fire events.
Remodulation Effect of Elateriospermum tapos Yoghurt on Metabolic Profile of Maternal Obesity Induced Cognitive Dysfunction and Anxiety-like Behavior in Female Offspring—An In Vivo Trial on Sprague Dawley Rats
Authors: Naomi Ruth , Rusli Rusydatul Nabila Mahmad , Teoh Soo Huat , Bahari Hasnah , Zakaria Zainul Amiruddin
Published: April 2023

Pre-pregnancy weight gain induces dysregulation in the metabolic profile of the offspring, thereby serving as a key factor for cognitive decline and anxiety status in the offspring. However, early probiotic supplementation during the gestational period is linked with improved metabolic health. At the same time, a natural plant known as Elateriospermum tapos (E. tapos) is proven to improve cognition and modulate the stress hormone due to its high concentration of flavonoids. However, the effects of medicinal plant integrated probiotics in F1 generations warrants further investigation. Thus, this study aimed to study the effect of E. tapos yoghurt on the maternal obesity induced cognitive dysfunction and anxiety in female offspring. In this study, female Sprague Dawley rats were fed with normal chow (n = 8) or high fat diet (n = 40) across pre-pregnancy, gestation, and weaning. The treatment with different concentrations of E. tapos yoghurt (5, 50, and 500 mg/kg/day) were initiated in the obese dams upon post coitum day 0 up to postnatal day 21 (PND 21). Female offspring were weaned on PND 21 and body mass index, waist circumference, lee index, behavior, metabolic parameter, and antioxidant status were analyzed. The result shows that the female offspring of the 500 mg/kg E. tapos yoghurt supplemented group shows a decreased level of insulin, fasting blood glucose, cholesterol, triglycerides, LDL, low fat tissue mass with a high level of HDL, and an increased level of antioxidant status in the hypothalamus. The behavioral assessment proves that the female offspring of the 500 mg/kg E. tapos yoghurt supplemented group exhibits a high recognition index on novel object/place with low anxiety-like behavior in an open field test. In conclusion, our data signify the beneficial effect of early intervention in obese dams on the transgenerational impact on female offspring’s metabolic profile, cognitive performance, and anxiety-like behavior.
One-Pot Self-Assembly of Core-Shell Nanoparticles within Fibers by Coaxial Electrospinning for Intestine-Targeted Delivery of Curcumin
Authors: Hou Lijuan , Zhang Laiming , Yu Chengxiao , Chen Jianle , Ye Xingqian , Zhang Fuming , Linhardt Robert J. , Chen Shiguo , Pan Haibo
Published: April 2023

Nanotechniques for curcumin (Cur) encapsulation provided a potential capability to avoid limitations and improve biological activities in food and pharmaceutics. Different from multi-step encapsulation systems, in this study, zein–curcumin (Z–Cur) core-shell nanoparticles could be self-assembled within Eudragit S100 (ES100) fibers through one-pot coaxial electrospinning with Cur at an encapsulation efficiency (EE) of 96% for ES100–zein–Cur (ES100–Z–Cur) and EE of 67% for self-assembled Z–Cur. The resulting structure realized the double protection of Cur by ES100 and zein, which provided both pH responsiveness and sustained release performances. The self-assembled Z–Cur nanoparticles released from fibermats were spherical (diameter 328 nm) and had a relatively uniform distribution (polydispersity index 0.62). The spherical structures of Z–Cur nanoparticles and Z–Cur nanoparticles loaded in ES100 fibermats could be observed by transmission electron microscopy (TEM). Fourier transform infrared spectra (FTIR) and X-ray diffractometer (XRD) revealed that hydrophobic interactions occurred between the encapsulated Cur and zein, while Cur was amorphous (rather than in crystalline form). Loading in the fibermat could significantly enhance the photothermal stability of Cur. This novel one-pot system much more easily and efficiently combined nanoparticles and fibers together, offering inherent advantages such as step economy, operational simplicity, and synthetic efficiency. These core-shell biopolymer fibermats which incorporate Cur can be applied in pharmaceutical products toward the goals of sustainable and controllable intestine-targeted drug delivery.
Standard-Compliant Gasoline by Upgrading a DTG-Based Fuel through Hydroprocessing the Heavy-Ends and Blending of Oxygenates
Authors: Graf David , Neuner Philipp , Rauch Reinhard
Published: April 2023

Methanol-to-gasoline (MTG) and dimethyl ether-to-gasoline (DTG) fuels are rich in heavy aromatics such as 1,2,4,5-tetramethylbenzene, resulting in low volatilities due to a lack of light ends, increased emission tendencies and drivability problems due to crystallization. Approaches addressing these issues mainly focus on single aspects or are optimized for petroleum-based feedstocks. This research article introduces an upgrading strategy for MTG and DTG fuels through hydroprocessing (HP) heavy-ends and applying a sophisticated blending concept. Different product qualities were prepared by HP heavy gasoline (HG) and Fischer-Tropsch wax using commercially available Pt/HZSM-5 and Pt/SAPO-11 catalysts in a fixed-bed reactor. The products were used for blending experiments, focusing on gasoline volatility characteristics. Accordingly, methanol, ethanol, methyl tert-butyl ether (MTBE), and ethyl tert-butyl ether (ETBE) were evaluated in a second blending experiment. The results were finally considered for preparing blends meeting EN 228. HP of HG was found to improve the amount of light-ends and the vapor pressure of the DTG fuel with increasing reaction temperature without, however, satisfying EN 228. The front-end volatility was further improved by blending methanol due to the formation of near-azeotropic mixtures, while ethyl tert-butyl ether (ETBE) considerably supported the mid-range volatility. A final blend with an alcohol content of less than 3 vol.%, mostly meeting EN 228, could be provided, making it suitable even for older vehicles.
Spatial-Temporal Heterogeneity in Large Three-Dimensional Nanofibrillar Cellulose Hydrogel for Human Pluripotent Stem Cell Culture
Authors: Hao Jin , Chen Ying , Zhu Mingjian , Zhao Yingqing , Zhang Kai , Xu Xia
Published: April 2023

One approach to cell expansion is to use large hydrogel for growing a large number of cells. Nanofibrillar cellulose (NFC) hydrogel has been used for human induced pluripotent stem cell (hiPSCs) expansion. However, little is known about the status of hiPSCs at the single cell level inside large NFC hydrogel during culture. To understand the effect of NFC hydrogel property on temporal–spatial heterogeneity, hiPSCs were cultured in 0.8 wt% NFC hydrogel with different thicknesses with the top surface exposed to the culture medium. The prepared hydrogel exhibits less restriction in mass transfer due to the presence of macropores and micropores interconnecting the macropores. More than 85% of cells at different depths survive after 5 days of culture inside 3.5 mm thick hydrogel. Biological compositions at different zones inside the NFC gel were examined over time at a single-cell level. A dramatic concentration gradient of growth factors estimated in the simulation along 3.5 mm NFC hydrogel could be a reason for the spatial–temporal heterogeneity in protein secondary structure and protein glycosylation and pluripotency loss at the bottom zone. pH change caused by the lactic acid accumulation over time leads to changes in cellulose charge and growth factor potential, probably another reason for the heterogeneity in biochemical compositions. This study may help to develop optimal conditions for producing high-quality hiPSCs in large nanofibrillar cellulose hydrogel at scale.
The Potential Biological Roles of Circular RNAs in the Immune Systems of Insects to Pathogen Invasion
Authors: Abbas Muhammad Nadeem , Kausar Saima , Gul Isma , Li Jisheng , Yu Huijuan , Dong Mengyao , Cui Hongjuan
Published: April 2023

Circular RNAs (circRNAs) are a newly discovered class of endogenously expressed non-coding RNAs (ncRNAs). They are highly stable, covalently closed molecules that frequently exhibit tissue-specific expression in eukaryotes. A small number of circRNAs are abundant and have been remarkably conserved throughout evolution. Numerous circRNAs are known to play important biological roles by acting as microRNAs (miRNAs) or protein inhibitors (‘sponges’), by regulating the function of proteins, or by being translated themselves. CircRNAs have distinct cellular functions due to structural and production differences from mRNAs. Recent advances highlight the importance of characterizing circRNAs and their targets in a variety of insect species in order to fully understand how they contribute to the immune responses of these insects. Here, we focus on the recent advances in our understanding of the biogenesis of circRNAs, regulation of their abundance, and biological roles, such as serving as templates for translation and in the regulation of signaling pathways. We also discuss the emerging roles of circRNAs in regulating immune responses to various microbial pathogens. Furthermore, we describe the functions of circRNAs encoded by microbial pathogens that play in their hosts.
Strategies for the Generation of Gene Modified Avian Models: Advancement in Avian Germline Transmission, Genome Editing, and Applications
Authors: Kim Young-Min , Woo Seung-Je , Han Jae-Yong
Published: April 2023

Avian models are valuable for studies of development and reproduction and have important implications for food production. Rapid advances in genome-editing technologies have enabled the establishment of avian species as unique agricultural, industrial, disease-resistant, and pharmaceutical models. The direct introduction of genome-editing tools, such as the clustered regularly interspaced short palindromic repeats (CRISPR) system, into early embryos has been achieved in various animal taxa. However, in birds, the introduction of the CRISPR system into primordial germ cells (PGCs), a germline-competent stem cell, is considered a much more reliable approach for the development of genome-edited models. After genome editing, PGCs are transplanted into the embryo to establish germline chimera, which are crossed to produce genome-edited birds. In addition, various methods, including delivery by liposomal and viral vectors, have been employed for gene editing in vivo. Genome-edited birds have wide applications in bio-pharmaceutical production and as models for disease resistance and biological research. In conclusion, the application of the CRISPR system to avian PGCs is an efficient approach for the production of genome-edited birds and transgenic avian models.
Comparative Transcriptomics in Atlantic Salmon Head Kidney and SHK-1 Cell Line Exposed to the Sea Louse Cr-Cathepsin
Authors: Leal Yeny , Valenzuela-Muñoz Valentina , Casuso Antonio , Benavente Bárbara P. , Gallardo-Escárate Cristian
Published: April 2023

The development of vaccines against sea lice in salmon farming is complex, expensive, and takes several years for commercial availability. Recently, transcriptome studies in sea louse have provided valuable information for identifying relevant molecules with potential use for fish vaccines. However, the bottleneck is the in vivo testing of recombinant protein candidates, the dosage, and the polyvalent formulation strategies. This study explored a cell-based approach to prospect antigens as candidate vaccines against sea lice by comparison with immunized fish. Herein, SHK-1 cells and Atlantic salmon head kidney tissue were exposed to the antigen cathepsin identified from the sea louse Caligus rogercresseyi. The cathepsin protein was cloned and recombinantly expressed in Escherichia coli, and then SHK-1 cell lines were stimulated with 100 ng/mL cathepsin recombinant for 24 h. In addition, Atlantic salmons were vaccinated with 30 ug/mL recombinant protein, and head kidney samples were then collected 30 days post-immunization. SHK-1 cells and salmon head kidney exposed to cathepsin were analyzed by Illumina RNA sequencing. The statistical comparisons showed differences in the transcriptomic profiles between SHK-1 cells and the salmon head kidney. However, 24.15% of the differentially expressed genes were shared. Moreover, putative gene regulation through lncRNAs revealed tissue-specific transcription patterns. The top 50 up and downregulated lncRNAs were highly correlated with genes involved in immune response, iron homeostasis, pro-inflammatory cytokines, and apoptosis. Also, highly enriched pathways related to the immune system and signal transduction were shared between both tissues. These findings highlight a novel approach to evaluating candidate antigens for sea lice vaccine development, improving the antigens screening in the SHK-1 cell line model.
Comparison of Secular Trends in Peptic Ulcer Diseases Mortality in China, Brazil and India during 1990–2019: An Age-Period-Cohort Analysis
Authors: Sun Jinyi , Huang Lihong , Li Ruiqing , Wang Tong , Wang Shuwen , Yu Chuanhua , Gong Jie
Published: April 2023

Background: Peptic ulcer disease (PUD) is a common disease worldwide, especially in developing countries. China, Brazil, and India are among the world’s fastest-growing emerging economies. This study aimed to assess long-term trends in PUD mortality and explore the effects of age, period, and cohort in China, Brazil, and India. Methods: We collected data from the 2019 Global Burden of Disease Study and used an age–period–cohort (APC) model to estimate the effects of age, period, and cohort. We also obtained net drift, local drift, longitudinal age curve, and period/cohort rate ratios using the APC model. Results: Between 1990 and 2019, the age-standardized mortality rates (ASMRs) of PUD and PUD attributable to smoking showed a downward trend in all countries and both sexes. The local drift values for both sexes of all ages were below zero, and there were obvious sex differences in net drifts between China and India. India had a more pronounced upward trend in the age effects than other countries. The period and cohort effects had a similar declining trend in all countries and both sexes. Conclusions: China, Brazil, and India had an inspiring decrease in the ASMRs of PUD and PUD attributable to smoking and to period and cohort effects during 1990–2019. The decreasing rates of Helicobacter pylori infection and the implementation of tobacco-restricting policies may have contributed to this decrease.
Effect of Muscle Energy Technique on Hamstring Flexibility: Systematic Review and Meta-Analysis
Authors: Kang Yeh-Hyun , Ha Won-Bae , Geum Ji-Hye , Woo Hyeonjun , Han Yun-Hee , Park Shin-Hyeok , Lee Jung-Han
Published: April 2023

Since 2005, there have been no systematic reviews on the effects of multiple manual therapies, including muscle energy technique (MET), on the hamstrings. Therefore, this systematic review aimed to provide clinical evidence for the effectiveness of the MET on hamstring flexibility. We queried 10 electronic databases (PubMed, EMBASE, The Cochrane Library, KISS, NDSL, KMBASE, KISTI, RISS, Dbpia, and OASIS) up to the end of March 2022. This study only included randomized controlled trials (RCTs) investigating the use of MET for the hamstring. The literature was organized using Endnote. Literature screening and data extraction were conducted by two researchers independently. The methodological quality of the included RCTs was evaluated using the Cochrane risk-of-bias tool 1.0, and the meta-analysis was performed using RevMan 5.4. In total, 949 patients from 19 RCTs were selected according to the inclusion criteria. During active knee extension tests, the efficacy between MET and other manipulations did not significantly differ. For sit and reach tests, MET groups had higher flexibility compared to stretching (MD = 1.69, 95% CI: 0.66 to 2.73, p = 0.001) and no treatment (MD = 2.02, 95% CI: 0.70 to 3.33, p = 0.003) groups. No significant differences were observed in the occurrence of adverse reactions. Overall, we found that MET is more efficacious for improving hamstring flexibility compared to stretching and having no treatment during sit and reach tests because it combines isometric contraction with stretching. Owing to clinical heterogeneity, uncertain risk of bias, and the small number of included studies, further high-quality studies should assess the effectiveness of MET.
Comparison between Arterial Blood Gases and Oxygen Reserve Index™ in a SCUBA Diver: A Case Report
Authors: Di Pumpo Fabio , Meloni Gualtiero , Paganini Matteo , Cialoni Danilo , Garetto Giacomo , Cipriano Alessandro , Giacon Tommaso Antonio , Martani Luca , Camporesi Enrico , Bosco Gerardo
Published: April 2023

Hypoxia and hyperoxia are both worrisome issues potentially affecting SCUBA divers, but validated methods to monitor these two conditions underwater are still lacking. In this experiment, a volunteer SCUBA diver was equipped with a pulse oximeter to detect peripheral oxygen saturation (SpO2) and a device to monitor the oxygen reserve index (ORi™). ORi™ values were compared with arterial blood oxygen saturation (SaO2) and the partial pressure of oxygen (PaO2) obtained from the cannulated right radial artery at three steps: at rest out of water; at −15 m underwater after pedaling on a submerged bike; after resurfacing. SpO2 and ORi™ mirrored the changes in SaO2 and PaO2, confirming the expected hyperoxia at depth. To confirm the potential usefulness of an integrated SpO2 and ORi™ device, further studies are needed on a broader sample with different underwater conditions and diving techniques.
A First Approach to the Study of Winsor & Newton’s 19th-Century Manufacture of Madder Red Lake Pigments
Authors: Veiga Tiago , Moro Artur J. , Nabais Paula , Vilarigues Márcia , Otero Vanessa
Published: April 2023

This paper focuses on the first investigation of the 19th-century manufacture of red lake pigments obtained from madder by Winsor & Newton (W&N), prominent artists’ colourman at that time. The first approach to their manufacture was carried out by studying the madder entries of the company’s book P1, found in the W&N 19th Century Archive Database. Eleven production records were discovered under names such as Rose Madder, Madder Carmine, Madder Lake and Madder Rose. Three main methods of synthesis were identified and reproduced, revealing three main steps: washing of the madder roots (Rubia tinctorum L.); extraction in acid media and complexation with Al3+ using alum; and precipitation by the addition of salts such as ammonium carbonate and sodium borate. The syntheses were followed by UV-VIS spectroscopy, and the pigments were further characterised by colourimetry, Energy-Dispersive X-Ray Fluorescence Spectrometry (XRF), Fourier Transform Infrared Spectroscopy (FTIR) and High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD). They all exhibited a rose hue in a highly insoluble aluminate matrix. Although the dye extraction was incomplete, alizarin, purpurin and pseudopurpurin were identified. An analytical comparison with a Rose Madder 19th-century oil paint tube was also performed by micro-FTIR and microspectrofluorimetry. This work intends to be foundational to a systematic study of the W&N’s 19th-century madder colours aiming to contribute new knowledge towards their identification and preservation in heritage objects.
Influence of Dose Conversions, Equilibrium Factors, and Unattached Fractions on Radon Risk Assessment in Operating and Show Underground Mines
Authors: Skubacz Krystian , Wołoszczuk Katarzyna , Grygier Agata , Samolej Krzysztof
Published: April 2023

This paper compares the results of measurements taken in the underground workings of active and tourist mines. In these facilities, the aerosol size distributions of ambient aerosols at key workplaces and the distributions of radioactive aerosols containing radon decay products were determined. Based on these studies, dose conversions used for dose assessment and unattached fractions were determined. In addition, radon activity concentrations and potential alpha energy concentrations of short-lived progeny were also measured in the mines to determine the equilibrium factor. The dose conversions varied between 2–7 mSv/(mJ × h × m−3). The unattached fraction measured in active coal mines ranged from 0.01–0.23, in tourist mines from 0.09–0.44, and in the tourist cave it was 0.43. The results showed significant discrepancies between the effective doses determined from current recommendations and legal regulations and those determined from direct measurements of parameters affecting exposure.
Muscular Fatigue and Quadriceps-to-Hamstring Ratio in Alpine Skiing in Women over 40 Years
Authors: Hermann Aljoscha , Christl Vera , Hastreiter Valentin , Carqueville Patrick , Ellenberger Lynn , Senner Veit
Published: April 2023

(1) Background: In alpine skiing, senior athletes and especially women have a high risk of knee injury. This may also be related to muscular fatigue (MF) of the knee-stabilizing thigh muscles. This study investigates both the evolution of muscle activity (MA) and of MF of the thighs throughout an entire skiing day. (2) Methods: n = 38 female recreational skiers over 40 years of age performed four specific skiing tasks (plough turns, V-steps uphill, turns with short, and middle radii) at specific times, while freely skiing the rest of the day. Surface EMG of the thigh muscle groups (quadriceps and hamstrings) was measured using special wearables (EMG pants). Apart from standard muscle activity parameters, the EMG data were also processed in the frequency domain to calculate the mean frequency and its shift over the day as a metric of muscle fatigue. (3) Results: The EMG pants showed reliable signal quality over the entire day, with BMI not impacting this. MF increased during skiing before and for both muscle groups significantly (p < 0.006) during lunch. MF, however, was not reflected in the quadriceps–hamstrings ratio. The plough manoeuvre seems to require significantly (p < 0.003) more muscle dynamics than the three other tasks. (4) Conclusion: MF may be quantified over an entire skiing day and thus fatigue information could be given to the skier. This is of major importance for skiers at the beginner level dominantly performing plough turns. Crucial for all skiers: There is no regenerative effect of a 45-min lunch break.
Knowledge, Attitude, Practice, and Perceived Barriers for the Compliance of Standard Precautions among Medical and Nursing Students in Central India
Authors: Sharma Megha , Bachani Rishika
Published: April 2023

Objectives: The aim of this study was to assess and compare (a) the knowledge, attitude, and practice of standard precautions (SPs), (b) the knowledge of post-exposure management, and (c) the perceived barriers underlying the noncompliance with SPs among future healthcare professionals (HCPs), i.e., students of medical and nursing courses in Central India. Setting and participants: A cross-sectional study was conducted in 2017–2018 among students of a medical and a nursing college using a pretested and modified questionnaire. Data were collected during 23 face-to-face sessions. Responses were scored according to standard guidelines of the Centers for Disease Control and Prevention and WHO, where each correct response was given a score of 1. Results: Among 600 participants, 51% of medical students and 75% of nursing students could not select the correct definition of SPs from the given options. Sixty-five percent of medical students (275/423) and 82% of nursing students (145/177) were unaware of the term post-exposure prophylaxis. Overall, knowledge about personal protective equipment and hazard symbols was poor (<25%). Furthermore, although theoretical knowledge about hand hygiene was good (510/600; 85%), its implementation was poor (<30%). Sixty-four percent of participants believed that the use of hand rub replaced the need for handwashing, even for visibly soiled hands. Some of the participants believed that the use of PPE might offend patients (16%). High workload and poor knowledge were other significant barriers underlying the noncompliance with SPs. Conclusions: A suboptimal translation of participants’ knowledge into practice is evident and signifies the presence of the know–do gap. Poor knowledge and inappropriate presumptions about the use of SPs discourage the practice of SPs. This results in increased healthcare-associated infections, increased treatment costs, and a suppressed social economy. The inclusion of a dedicated curriculum with repeated hands-on and practice-based training on SPs is suggested to minimize this know–do gap among future healthcare workers.
Socio-Economic Inequalities in the Double Burden of Malnutrition among under-Five Children: Evidence from 10 Selected Sub-Saharan African Countries
Authors: Alaba Olufunke A. , Chiwire Plaxcedes , Siya Aggrey , Saliu Oluremi A. , Nhakaniso Karen , Nzeribe Emmanuella , Okova Denis , Lukwa Akim Tafadzwa
Published: April 2023

Background: Africa is unlikely to end hunger and all forms of malnutrition by 2030 due to public health problems such as the double burden of malnutrition (DBM). Thus, the aim of this study is to determine the prevalence of DBM and degree of socio-economic inequality in double burden of malnutrition among children under 5 years in sub-Saharan Africa. Methods: This study used multi-country data collected by the Demographic and Health Surveys (DHS) Program. Data for this analysis were drawn from the DHS women’s questionnaire focusing on children under 5 years. The outcome variable for this study was the double burden of malnutrition (DBM). This variable was computed from four indicators: stunting, wasting, underweight and overweight. Inequalities in DBM among children under 5 years were measured using concentration indices (CI). Results: The total number of children included in this analysis was 55,285. DBM was highest in Burundi (26.74%) and lowest in Senegal (8.80%). The computed adjusted Erreygers Concentration Indices showed pro-poor socio-economic child health inequalities relative to the double burden of malnutrition. The DBM pro-poor inequalities were most intense in Zimbabwe (−0.0294) and least intense in Burundi (−0.2206). Conclusions: This study has shown that across SSA, among under-five children, the poor suffer more from the DBM relative to the wealthy. If we are not to leave any child behind, we must address these socio-economic inequalities in sub-Saharan Africa.
Effects of Health Anxiety, Social Support, and Coping on Dissociation with Mediating Role of Perceived Stress during the COVID-19 Pandemic
Authors: Kolozsvári László Róbert , Rekenyi Viktor , Garbóczy Szabolcs , Hőgye-Nagy Ágnes , Szemán-Nagy Anita , Sayed-Ahmad Mohamed , Héjja-Nagy Katalin
Published: April 2023

Background: Our study aimed to examine whether health anxiety, social support, and ways of coping relate to dissociation directly or only through the mediation of perceived stress, moderated by the time of measurement (lockdown). We investigated the effect of perceived stress on different forms (sub-scales) of dissociation. Methods: A cross-sectional survey was conducted by an online form at two points in time: the beginning and the later stage of the COVID-19 pandemic. Results: We received a total of 1711 responses. Perceived stress moderately correlated with dissociation in both international and Hungarian samples. Health anxiety showed a strong direct and indirect correlation with dissociation. Regarding social support, the support of family significantly decreased the dissociative experiences in the Hungarian sample mediated by perceived and direct stress. In the international sample, goal-oriented coping strategies strongly decreased all dissociation scales in the first measurement, through the mediation of perceived stress. As for the Hungarian sample, positive thinking was found to decrease dissociation by decreasing perceived stress. Conclusion: health anxiety, coping, and social support appeared to influence dissociation directly and through the mediation of perceived stress. Social support, mainly support of the family and problem-focused coping strategies may decrease the level of stress, this way decreasing dissociative behavior.
Measuring the Impact of Bedroom Privacy on Social Networks in a Long-Term Care Facility for Hong Kong Older Adults: A Spatio-Social Network Analysis Approach
Authors: Yang Aria C. H. , Chaudhury Habib , Ho Jeffrey C. F. , Lau Newman
Published: April 2023

This study aims to measure the impact of bedroom privacy on residents’ social networks in a long-term care (LTC) facility for older adults. Little is known about how the architectural design of bedrooms affects residents’ social networks in compact LTC facilities. Five design factors affecting privacy were examined: bedroom occupancy, visual privacy, visibility, bedroom adjacency, and transitional space. We present a spatio-social network analysis approach to analyse the social network structures of 48 residents. Results show that residents with the highest bedroom privacy had comparatively smaller yet stronger groups of network partners in their own bedrooms. Further, residents who lived along short corridors interacted frequently with non-roommates in one another’s bedrooms. In contrast, residents who had the least privacy had relatively diverse network partners, however, with weak social ties. Clustering analyses also identified five distinct social clusters among residents of different bedrooms, ranging from diverse to restricted. Multiple regressions showed that these architectural factors are significantly associated with residents’ network structures. The findings have methodological implications for the study of physical environment and social networks which are useful for LTC service providers. We argue that our findings could inform current policies to develop LTC facilities aimed at improving residents’ well-being.
The Effects of 12-Week Dual-Task Physical–Cognitive Training on Gait, Balance, Lower Extremity Muscle Strength, and Cognition in Older Adult Women: A Randomized Study
Authors: Nascimento Marcelo de Maio , Maduro Paula Andreatta , Rios Pâmala Morais Bagano , Nascimento Lara dos Santos , Silva Carolina Nascimento , Kliegel Matthias , Ihle Andreas
Published: April 2023

This study aims to investigate the effects of dual-task physical–cognitive the training on body balance (BB), gait performance (GP), lower limb muscle strength (LEMS), and cognitive performance (CP) in a group of cognitively normal older adult women (n = 44; 66.20 ± 4.05 years). Of these, 22 were randomly allocated to the dual-task training (DT) group, and 22 participated in the control group (CG). Assessments were performed at baseline, after 12 weeks of intervention, and at the end of 12 weeks of follow-up, using the following instruments: Timed Up & Go (TUG), Timed Up & Go manual (TUGm), Timed Up & Go cognitive (TUGc), Balance Test (TEC), sit-to-stand test (STS), and verbal fluency test (VF). After 12 weeks of DT training, participants showed a significant time × group interaction in all motor assessments (BB, GP, LEMS), as well as in three cognitive tests (VF-grouping, VF-exchange, VF-total). No time–group interaction effect was indicated for the VF-category test. At all evaluation times, CG members maintained constant physical and cognitive performance. We conclude that 12 weeks of physical–cognitive DT training was effective in promoting BB, GP, and LEMS, as well as CP in cognitively normal older adult women, with lasting effects up to 12 weeks after the intervention.
Osteogenic Differentiation Effect of Human Periodontal Ligament Stem-Cell Initial Cell Density on Autologous Cells and Human Bone Marrow Stromal Cells
Authors: Wang Jing , Qiao Qingchen , Sun Yaxi , Yu Wenting , Wang Jiran , Zhu Minjia , Yang Kai , Huang Xiaofeng , Bai Yuxing
Published: April 2023

Stem cells have differentiation and regulation functions. Here, we discussed the impact of cell culture density on stem cell proliferation, osteoblastogenesis, and regulation. To discuss the effect of the initial culture density of human periodontal ligament stem cells (hPDLSCs) on the osteogenic differentiation of autologous cells, we found that the hPDLSC proliferation rate decreased with an increase in the initial plating density (0.5–8 × 104 cells/cm2) for the 48 h culture cycle. After hPDLSCs induced osteogenic differentiation for 14 days with different initial cell culture densities, the expression of osteoprotegerin (OPG) and runt-related transcription factor 2(RUNX2) and the OPG/ Receptor Activator of Nuclear Factor-κ B Ligand (RANKL) ratio were the highest in the hPDLSCs initially plated at a density of 2 × 104 cells/cm2, and the average cell calcium concentration was also the highest. To study hPDLSCs regulating the osteoblastic differentiation of other cells, we used 50 μg/mL of secreted exosomes derived from hPDLSCs cultured using different initial cell densities to induce human bone marrow stromal cell (hBMSC) osteogenesis. After 14 days, the results indicated that the gene expression of OPG, Osteocalcin(OCN,)RUNX2, and osterix and the OPG/RANKL ratio were the highest in the 2 × 104 cells/cm2 initial cell density group, and the average calcium concentration was also the highest. This provides a new idea for the clinical application of stem cell osteogenesis.
Advances in the Electrophysiological Recordings of Long-Term Potentiation
Authors: Jiang Feixu , Bello Stephen Temitayo , Gao Qianqian , Lai Yuanying , Li Xiao , He Ling
Published: April 2023

Understanding neuronal firing patterns and long-term potentiation (LTP) induction in studying learning, memory, and neurological diseases is critical. However, recently, despite the rapid advancement in neuroscience, we are still constrained by the experimental design, detection tools for exploring the mechanisms and pathways involved in LTP induction, and detection ability of neuronal action potentiation signals. This review will reiterate LTP-related electrophysiological recordings in the mammalian brain for nearly 50 years and explain how excitatory and inhibitory neural LTP results have been detected and described by field- and single-cell potentials, respectively. Furthermore, we focus on describing the classic model of LTP of inhibition and discuss the inhibitory neuron activity when excitatory neurons are activated to induce LTP. Finally, we propose recording excitatory and inhibitory neurons under the same experimental conditions by combining various electrophysiological technologies and novel design suggestions for future research. We discussed different types of synaptic plasticity, and the potential of astrocytes to induce LTP also deserves to be explored in the future.
The Role of Nitric Oxide in the Micro- and Macrovascular Response to a 7-Day High-Salt Diet in Healthy Individuals
Authors: Tolj Ivana , Stupin Ana , Drenjančević Ines , Šušnjara Petar , Perić Leon , Stupin Marko
Published: April 2023

This study aimed to investigate the specific role of nitric oxide (NO) in micro- and macrovascular response to a 7-day high-salt (HS) diet, specifically by measuring skin microvascular local thermal hyperemia and the flow-mediated dilation of the brachial artery, as well as serum NO and three NO synthase enzyme (NOS) isoform concentrations in healthy individuals. It also aimed to examine the concept of non-osmotic sodium storage in the skin following the HS diet by measuring body fluid status and systemic hemodynamic responses, as well as serum vascular endothelial growth factor C (VEGF-C) concentration. Forty-six young, healthy individuals completed a 7-day low-salt diet, followed by a 7-day HS diet protocol. The 7-day HS diet resulted in impaired NO-mediated endothelial vasodilation in peripheral microcirculation and conduit arteries, in increased eNOS, decreased nNOS, and unchanged iNOS concentration and NO serum level. The HS diet did not change the volume of interstitial fluid, the systemic vascular resistance or the VEGF-C serum level. These results indicate that the 7-day HS-diet induces systemic impairment of NO-mediated endothelial vasodilation, while dissociation in the eNOS and nNOS response indicates complex adaptation of main NO-generating enzyme isoforms to HS intake in healthy individuals. Our results failed to support the concept of non-osmotic sodium storage.
The Osteogenic Properties of Calcium Phosphate Cement Doped with Synthetic Materials: A Structured Narrative Review of Preclinical Evidence
Authors: Md Dali Siti Sarah , Wong Sok Kuan , Chin Kok-Yong , Ahmad Fairus
Published: April 2023

Bone grafting is commonly used as a treatment to repair bone defects. However, its use is challenged by the presence of medical conditions that weaken the bone, like osteoporosis. Calcium phosphate cement (CPC) is used to restore bone defects, and it is commonly available as a bioabsorbable cement paste. However, its use in clinical settings is limited by inadequate mechanical strength, inferior anti-washout characteristics, and poor osteogenic activity. There have been attempts to overcome these shortcomings by adding various natural or synthetic materials as enhancers to CPC. This review summarises the current evidence on the physical, mechanical, and biological properties of CPC after doping with synthetic materials. The incorporation of CPC with polymers, biomimetic materials, chemical elements/compounds, and combination with two or more synthetic materials showed improvement in biocompatibility, bioactivity, anti-washout properties, and mechanical strength. However, the mechanical property of CPC doped with trimethyl chitosan or strontium was decreased. In conclusion, doping of synthetic materials enhances the osteogenic features of pure CPC. The positive findings from in vitro and in vivo studies await further validation on the efficacy of these reinforced CPC composites in clinical settings.