bluuebunny/crossref_metadata_2025 huggingface dataset:
STABILITY ASSESSMENT OF ROOT-REINFORCED SLOPES USING FINITE ELEMENT LIMIT ANALYSIS
Authors: Hutama Dio Alif , Farichah Himatul
Published: June 2024

Slope instability poses serious threats to infrastructure and environmental sustainability. As a result, numerous reinforcement techniques have been used as disaster mitigation attempts to prevent slope failure. Among various traditional slope reinforcement methods, the use of vegetion root is more cost-effective and environmentally friendly. This paper presents stability assessment of root-reinforced slopes. Firstly, slope models were built for case of bare and root-reinforced slopes. The slope angles were varied in the range of 15º ~ 55 º. The root cohesion and depth of root zone were adjusted within the ranges of 0 ~ 20 kPa and 0 ~1.5 m, respectively. The strength reduction finite element limit analysis method was applied to evaluate the stability of the slopes in this study. The results indicate that factor of safety increases with the increasing of root cohesion and depth of root zone. The best factor of safety was obtained for the case with root cohesion and depth of root zone of 20 kPa and 1.5 m, respectively. Shear dissipation contours of the slope models also show that root reinforcement reduces shear dissipation energy along the failure surface, consequently lowering the possibility of slope failure.
ARE VOCATIONAL SCHOOL STUDENTS READY TO FACE WORKFORCE DISRUPTION IN THE CONSTRUCTION FIELD?
Authors: Arfandi Anas , Tahir Lopa Akshari , Hasbi
Published: June 2024

The purpose of this research is to 1) Describe the digital literacy understanding of vocational high school students in the Construction and Property Engineering program. 2) Analyze students' understanding of technological disruption in the field of construction. 3) Analyze students' readiness to face technological disruptions in the construction field. The research was conducted at a vocational high school that offers the Construction and Property Engineering program in South Sulawesi province. The study took place from March to June 2023. The population of the research consisted of all students in grade X, totaling 1125 students. The sample size was determined using the Slovin formula with a 0.05 level of error, resulting in a sample of 318 respondents. All items are valid and reliable based on the validity and reliability test with a value greater than 0.361. The research findings indicate that the digital literacy understanding of vocational high school students in the Construction and Property Engineering program regarding their readiness to face the labor workforce disruption in the construction field is mostly categorized as very good with a percentage of 80%. Furthermore, students' understanding of technological disruption in the construction field and their readiness to face the labor workforce disruption in the construction field is dominantly categorized as very good with a percentage of 63%. Student readiness is dominantly in the Able category, but there are still 18% who are in the Need to optimize category in facing technological disruption in the construction sector.
Nuclear Governance Assessing Effectiveness and Enhancing International Cooperation
Authors: Nishonov Abdulloh Ubaydulloh o’g’li
Published: June 2024

This article explores the legal regulation of atomic energy, focusing on its historical evolution, current status, and effectiveness. It addresses gaps in understanding regarding the adequacy of existing regulations and the extent of international cooperation. Employing a multifaceted approach, including documentary analysis and interviews, the study assesses legislative frameworks and institutional mechanisms. Findings reveal the complexities of atomic energy governance and underscore the importance of international cooperation in enhancing regulatory compliance. This research provides valuable insights for policymakers and stakeholders, facilitating informed strategies to strengthen nuclear governance and ensure safe and sustainable atomic energy utilization.
Highlights: 

Comprehensive assessment of legal regulations governing atomic energy.
Importance of international cooperation in enhancing regulatory compliance.
Implications for strengthening nuclear governance and promoting sustainability.

Keywords: Atomic Energy, Legal Regulation, International Cooperation, Governance, Agreements
Investigating the Role of Serum Hepcidin and Interleukin-6 in Non-Anemic Women with Acute Ischemic Stroke
Authors: Hussain Zahir
Published: June 2024

Background: Hepcidin (HP) is an important regulator of iron homeostasis. Iron status and IL-6 have been shown to regulate the expression of HP. Serum iron (SI), HP, and IL-6 have a highly significant role in inflammation since inflammation elevates the levels of HP for expressing the ischemic condition. The present study was carried out to investigate the impact of an interactive association between HP, SI, and IL-6 in non-anemic women with acute ischemic stroke (AIS). Methods and Results: The present case-control, descriptive study comprised 25 non-anemic women with AIS and 25 healthy non-anemic women controls. The age range of AIS and control subjects was 50 to 54 years. Non-anemic AIS women within 8 hours after AIS onset (AIS<8 hrs) and 72 hours after onset of AIS (AIS-72 hours) were examined. The patients underwent assessment of traditional risk factors, physical examination, CBC, blood biochemistry test, 12-lead ECG, CT scans, MRI, CT or MR angiogram, carotid ultrasound of the arteries, transcranial doppler ultrasound, and EEG. Office blood pressure was measured using a mercury sphygmomanometer. Significantly increased values were obtained for systolic blood pressure and diastolic blood pressure in AIS women, compared to the control subjects: 137.44±12.35 vs. 130.88±7.30 mmHg (P=0.0267) and 86.72± 8.48 vs. 81.80± 5.42 mmHg (P=0.0183), respectively. Serum CRP and LDL-C levels in AIS were significantly higher than in healthy control women (P<0.0001). A significant increase in serum HP, SI, and IL-6 for AIS<8 hrs was found, compared to AIS-72 hrs (P<0.001 in all cases). Comparison for AIS<8 hrs vs. controls showed a highly significant increase in serum HP, SI, and IL-6 for AIS<8 hrs (P<0.001 in all cases). On the other hand, AIS-72 hrs vs. controls indicated a significant increase in SI (P=0.0028) and IL-6 (P=0.0065) but a non-significant increase in serum HP (P>0.05) in AIS-72 hrs. Linear regression expressed a high strength of the relationship between serum HP and SI for AIS<8 hrs (R2=0.82, P<0.0001) and AIS-72 hrs (R2=0.73, P<0.0001), but a negligible linear association for controls (R2=0.01, P=0.5990). There was a high relationship between HP and IL-6 for AIS<8 hrs (R2=0.67, P<0.0001) and AIS-72 hrs (R2=0.67, P<0.0001), but a small linear association for controls (R2=0.28, P=0.0063). The R2 value of 0.47 (P<0.0002) and 0.42 (P<0.0004) was found between SI and IL-6 for AIS<8 hrs and AIS-72 hrs, and a negligible linear association (R2=0.006, P=0.7250) for controls. Conclusion: The present study provides evidence of the association of AIS in non-anemic women with increased hepcidin. The interactive pathophysiological role of HP, IL-6, and SI in non-anemic women with AIS has also been shown.
A Narrative Review of the Impacts of Obesity on Pulmonary Function and Muscle Strength
Authors: Alzahrani Ahad , Ezzat Wessam , Abdelbasset Walid
Published: June 2024

Obesity has reached alarming proportions worldwide, requiring close attention and comprehensive knowledge about its health consequences. Obesity today affects people of all ages and socioeconomic levels. This narrative review is devoted to the multifaceted impacts of obesity on pulmonary function and muscle strength. Obesity, characterized by excessive fat accumulation, induces complex physiological changes, transforming adipose tissue into a metabolically dynamic organ. The multifarious nature of obesity causes complicated physiological alterations that affect the whole body. Besides its well-known impacts on metabolic health, obesity, particularly abdominal obesity, challenges the respiratory system mechanically. This review navigates through the mechanical challenges that obesity poses to pulmonary function, elucidating how excess adipose tissue in the abdominal region compromises lung expansion and increases the workload on respiratory muscles. Simultaneously, the review explores the dynamic interplay between obesity and muscle strength. Obesity and muscle strength are linked by metabolic dysfunction, muscle composition changes, and lifestyle variables. Clinical implications of obesity extend beyond metabolic consequences, emphasizing impaired pulmonary function and diminished muscle strength as crucial determinants of clinical outcomes. A multidisciplinary approach involving collaboration among healthcare professionals is advocated, addressing the physiological and psychological factors contributing to obesity.
Prognostic Value of Melatonin in Patients with Chronic Obstructive Pulmonary Disease
Authors: Budnevsky Andrey , Avdeev Sergey , Ovsyannikov Evgeniy , Vostrikova Karina , Budnevskaya Sofiya , Kitoyan Avag
Published: June 2024

The article outlines a detailed examination of multiple scientific studies investigating how melatonin affects COPD's advancement and growth. The severity of oxidative stress in COPD, a complex disease with multiple factors, is significantly reduced by melatonin by activating intracellular antioxidants, as suggested by the data acquired. Considering the available data, we can conclude that melatonin has a protective role against oxidative stress in COPD patients in addition to regulating the circadian rhythm.
How Much Radiation Are Women in Saudi Arabia Receiving from Mammography? A Review
Authors: Albahiti Sarah
Published: June 2024

This review compiles and assesses data from recent studies on mammographic radiation doses in Saudi Arabia, aiming to evaluate mean glandular dose (MGD) exposure during mammography and its implications in breast cancer risk. The reviewed studies spanned from 2019 to 2023 and included a range of sample sizes and institutional settings, with patients’ ages from 27 to 85 years. Considerations such as the number of mammographic views and compressed breast thickness were examined. The studies reported average MGDs below the National Diagnostic Reference Level set by the Saudi Food and Drug Authority. However, limitations were noted regarding sample size selection and incomplete data on all mammographic projections. Despite these limitations, the findings highlight the need for continued assessment of patient doses to optimize mammography practices and address the absence of quality standardization acts in Saudi Arabia. These insights are critical for governing authorities to ensure that effective patient dose monitoring occurs regularly and that the establishment of minimum quality standards for breast cancer screening is intact.
Silver in Wound and Trophic Ulcer Treatment: A Modern View of the Problem
Authors: Hryvenko Serhiy , Golomidov Aleksander , Sidikov Sydyk , Margarian Manvel , Bostanova Madina , Salpagarova Zaineb
Published: June 2024

This review presents published data on the history of use, mechanism of action, and effectiveness of silver and silver-based drugs in surgical practice. A literature search was carried out using PubMed, EMBASE, Google Scholar, and E-Library databases. Analysis of available literature data convincingly demonstrates the effectiveness of silver nanocomposites as antibacterial and anti-inflammatory agents. The demonstrated antimicrobial property of silver nanoparticles (AgNPs) against various antibiotic-resistant bacteria is especially significant for clinical use. Silver nanoparticles have clinically proven effective in treating wounds and trophic ulcers.
Construction of Physical Education Evaluation System Based on Fuzzy Neural Network Modeling
Authors: Wang Hua Wang Hua
Published: June 2024

As a frontier concept of current basic education, subject literacy indicates that our country has established the direction of curriculum reform aiming at developing students' core literacy. The core subject literacy is not only the basic subject literacy gradually developed by students in the course of subject learning, but also the comprehensive factors such as character and ability gradually formed by students in extracurricular social practice activities, which is an important indicator of the new curriculum education reform. In view of the fact that the special teaching connotation of high school physical education still needs to be clarified, the evaluation methods of teaching effect still need to be enriched, and the teaching reform theory needs to be further improved. Focus on cultivating college students' lifelong sports awareness, mastering sports knowledge and skills, and developing their physical fitness, good sports behavior and sports moral quality healthily. According to the characteristics of physical education learning evaluation, the fuzzy neural network is selected to model, and the physical education teaching evaluation system is constructed. The adaptive fuzzy neural system is used to process the data, thus realizing the intelligence and automation of physical education teaching evaluation. Finally, the artificial neural network and adaptive fuzzy neural system are used in the evaluation and modeling of physical education teaching, and the experimental simulation comparison and performance analysis of the two models show that the model has good evaluation ability.
m1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19
Authors: Mi Shuyi , Hu Jie , Chen Wenwen , Chen Jingyu , Xu Zhipeng , Xue Meng
Published: June 2024

Background: In recent years, the emphasis has shifted to understanding the role of N1-methyladenosine (m1A) in tumor progression as little is known about its regulatory effect on mRNA and its role in the metastasis of colorectal cancer (CRC).
Methods: We performed methylated RNA immunoprecipitation sequencing of tumor tissues and tumor-adjacent normal tissues from three patients with CRC to determine the m1A profile of mRNA in CRC. The expression of diaphanous-related formin 3 (DIAPH3) and its correlation with clinicopathological characteristics of CRC were evaluated using immunohistochemistry and online datasets. The role of DIAPH3 in the migration and invasion of CRC cells was evaluated using wound healing assay, Transwell assay and xenograft metastatic model. The downstream targets of DIAPH3 were screened using mass spectrometry. By co-transfecting DIAPH3 siRNA and a keratin 19 (KRT19) ectopic plasmid into CRC cells, the role of DIAPH3-KRT19 signaling axis was confirmed.
Results: The mRNA level of DIAHP3 and its m1A modifications increased simultaneously in the CRC tissues. In addition, high DIAPH3 expression in CRC tissues is significantly associated with metastasis and progression to an advanced stage. After the knockdown of DIAPH3, the migration and invasion capabilities of CRC cells suffered a notable decline, which could be rescued by overexpressing KRT19. In addition, the proteasome inhibitor MG132 could block the degradation of KRT19 induced by DIAPH3 silencing.
Conclusions: Our study reveals that DIAPH3 mRNA was modified in CRC cells by m1A methylation. Silencing DIAPH3 suppresses the migration and invasion of CRC cells, potentially through the proteasome-dependent degradation of downstream KRT19.
The multiwavelength behaviour of BL Lacertae explained by a wiggling filamentary jet
Authors: Raiteri Claudia
Published: June 2024

Blazars are active galactic nuclei that stand out for their extreme flux variability at all frequencies, from the radio to the γ-ray band. Their emission is dominated by the non-thermal radiation coming from a relativistic jet pointing close to the line of sight, with consequent Doppler beaming. This in particular implies an apparent increase of the source brightness and a shortening of the variability time scales. Many authors have explained the flares observed in the blazar light curves in terms of intrinsic energetic processes such as shocks propagating in the jet, magnetic reconnection, and turbulence. Alternative interpretations take into consideration possible geometric effects, due to changes in the orientation of the jet, as in the case of the twisting jet model proposed in Raiteri et al. (2017, Nature 552, 374). These geometrical models are supported by several observations and numerical simulations. Recent space-VLBI radio images of the source 3C 279 suggest that blazar emission comes from rotating filamentary structures produced by plasma instabilities (Fuentes et al. 2023, Nature Astronomy, 7, 1359). Moreover, mm-VLBI imaging observations of BL Lacertae found evidence of a wiggling helical jet structure close to the core (Kim et al. 2023, Astron. & Astrophys. 680, L3), confirming earlier results (Cohen et al. 2015, Astrophys. J. 803, 3). In 2020–2021 BL Lacertae underwent an extraordinary activity phase leading to its historical optical maximum brightness (Raiteri et al. 2023, Mon. Not. R. Astron. Soc. 522, 102), providing a formidable tool to study blazar variability. In the present work, we explain the multiwavelength behaviour of BL Lacertae in terms of a twisting filamentary jet, whose better alignment with the line of sight produces the observed flares. The exceptionally well-sampled optical light curve by the Whole Earth Blazar Telescope (WEBT) is used together with radio data to reconstruct the motion of the jet emitting regions in time. The γ-ray data from the Fermi satellite confirm the geometrical picture and allow us to assess the synchrotron self-Compton nature of the γ-ray radiation. This means that γ-ray photons are produced by inverse-Compton scattering of optical photons off the same relativistic electrons that are responsible for the optical radiation through a synchrotron process. The flux behaviour of other blazars suggests that the twisting filamentary jet model can provide an explanation for the multiwavelength variability of the whole blazar class.
Cysteine allostery and autoinhibition govern human STING oligomer functionality
Authors: Li Lingyin , Chan Rebecca , Cao Xujun , Ergun Sabrina , Njomen Evert , Lynch Stephen , Ritchie Christopher , Cravatt Benjamin
Published: June 2024

The anti-viral and anti-cancer STING innate immune pathway can exacerbate autoimmune and neurodegenerative diseases when aberrantly activated, emphasizing a key unmet need for STING pathway antagonists. However, no such inhibitors have advanced to the clinic because it remains unclear which mechanistic step(s) of human STING activation are crucial for potent and context-independent inhibition of downstream signaling. Here, we report that C91 palmitoylation, the mechanistic target of a potent tool compound, is not universally necessary for human STING signaling, making it a poor target for drug development. Instead, we discover that evolutionarily conserved C64 is basally palmitoylated and is crucial for preventing unproductive STING oligomerization in the absence of cGAMP stimulation. The effects of palmitoylation at C64 and C91 converge on the control of intra-dimer disulfide bond formation at C148. Importantly, we show for the first time that signaling-competent STING oligomers are composed of a mixture of two species: disulfide-linked STING dimers that stabilize the oligomer, and reduced STING dimers that are phosphorylated to actuate interferon signaling. Given this complex landscape and cell type specificity of palmitoylation modifications, we conclude that robust STING inhibitors must directly inhibit the oligomerization process. Taking inspiration from STING’s natural autoinhibitory mechanism, we identified an eight amino acid peptide that binds a defined pocket at the inter-dimer oligomerization interface as a proof-of-concept human STING inhibitor, setting the stage for future therapeutic development.
Simulation of flood associated with extreme rainfall events over the urban city, Bengaluru, India
Authors: Purwar Smrati , Mohapatra Gyanendranath , V Rakesh
Published: June 2024

Bangalore city witnessed many flash floods associated with extreme rainfall events (EREs) in the recent past. Our analysis showed an increasing trend in the annual and monthly average rainfall over major valleys in Bengaluru Urban from 1971 to 2020 which indicated the possibility of rise in the frequency of urban flooding in recent decades. The digital elevation map (DEM) is examined to understand the topography of high elevation and low-lying areas, enabling the assessment of flood impacts of EREs in various areas of Bengaluru Urban. We have selected four flash flood events associated with EREs over one of the critically low laying Koramangala-Challaghatta watershed valley (KC valley) in the city for our simulation study. To understand the flood pattern, timing and volume during these flood events, stormwater management model (SWMM) simulations were conducted with observed rainfall from rain gauge stations. The model simulations could capture the inundation prone regions of KC valley for the flood events of different rainfall intensity. The hyetograph of the rainfall runoff with elapsed time demonstrates the model capability to calculate the runoff amount for different intensity of EREs. The bias in simulated storm water discharge range from +4 to -4 cubic meter per second (CMS) at different time scales and intensities within specific areas of stormwater drainage network system. The flood modeling systems like the one presented in this study can serve as valuable decision support system for management of urban floods associated with EREs and planning of mitigation strategies.
Recurrence risk of basal cell carcinoma of the eyelid with regard to demographic and clinical patient data
Authors: Wisdorf Konstantin , Rokohl Alexander C. , Fan Wanlin , Heindl Ludwig M.
Published: June 2024

Purpose: To describe the demographic features of patients affected by basal cell carcinomas of the eyelid and the clinical features of the tumors, as well as to examine the correlation between these features and the recurrence rate.
Methods: This is a retrospective series of 118 cases of basal cell carcinoma of the eyelid that underwent surgical excision between January 1, 2009, and December 31, 2015. The inclusion criteria were a minimum follow-up of one year and a histopathological confirmed basal cell carcinoma. The study recorded demographic and clinical characteristics. These characteristics were then statistically analyzed to determine any significant correlation to the recurrence rate.
Results: 20 patients (16.95%) experienced tumor recurrence, with one case recurring twice during the observation period. Out of 118 cases, all but two basal cell carcinomas presented clear resection margins after one or more excisions. None of the characteristics of the patients and basal cell carcinomas showed a significant correlation to the recurrence rate (p values <0.05). Cases with sclerodermiform and superficial subtypes, recurrent basal cell carcinomas, positive resection margins or horizontal tumor diameters more than 5mm presented higher recurrence rates than other cases.
Conclusion: Standard surgical procedures can achieve low recurrence rates, provided that the resection margins are clear. In a sample with a comparatively low rate of positive resection margins (1.69%) after the operation, none of the analyzed variables were found to significantly correlate with the recurrence rate. This highlights the importance of clear resection margins in the surgical therapy of basal cell carcinoma.
Serum secreted EMC10 (scEMC10) levels are inversely associated with metabolically active brown adipose tissue in humans
Authors: Wang Xuanchun , Miao Qing , Wang Yahao , Zhang Qiongyue , Wu Wei , Yu Yifei , Zeng Fangfang , Yang Yehong , Zuo Chuantao , Guan Yihui , Liew Chong Wee , Blüher Matthias , Li Yiming
Published: June 2024

Background/Objectives: Secreted endoplasmic reticulum membrane complex subunit 10 (scEMC10) has been implicated in obesity in mice and humans. In this study, the associations of serum scEMC10 levels with thermoneutrality-modulated brown adipose tissue (BAT) activity and thyroid hormone (TH)-dependent thermogenesis were investigated in humans.
Subjects/Methods: Serum scEMC10 levels were measured in participants from manifold cohorts using enzyme-linked immunosorbent assay, including participants with or without active BAT determined by PET-CT scanning, BAT-positive participants before and after thermoneutrality, and patients with hyperthyroidism before and after an anti-thyroid drug treatment. The difference in serum scEMC10 between BAT-positive and -negative participants, and the changes of serum scEMC10 in BAT-positive participants before and after thermoneutrality and in patients with Grave’s disease-caused hyperthyroidism before and after an anti-thyroid drug (ATD) treatment were determined.
Results: 18F-FDG PET–CT scanning indicated BAT-positive participants were significantly younger and leaner than BAT-negative ones. There was, however, no significant difference in serum scEMC10 between the two groups. Serum scEMC10 levels in BAT-positive participants were significantly elevated by 2-hour thermoneutrality (P = 0.0017), concomitant with disappearance of active BAT. No significant association of serum scEMC10 with serum levels of either TSH, FT3, or FT4 was observed in participants from both Chinese and White cohorts. ATD treatment normalized thyroid function and reduced the uptake of 18F-FDG in skeletal muscle of patients with hyperthyroidism. Serum scEMC10 concentration, however, remained unchanged in these patients before and after ATD treatment.
Conclusions: Serum scEMC10 levels are inversely associated with metabolically active BAT, suggesting scEMC10 a suppressive role in facultative thermogenesis in humans. This finding implicates inhibition of scEMC10 in the treatment of obesity and its associated cardiometabolic diseases.
The Impact of Benson's Relaxation Technique on the Quality of Life of Operating Room Nurses in Hospitals Southwest Iran: A Randomized Controlled Trial
Authors: Mohebbi Zinat , Ghanbarzadeh Sina , Ejraei Alireza , Gheisari Somaye , Hosseini Fahimeh Alsadat
Published: June 2024

Background In clinical settings, continuous changes lead to increased professional stress, affecting the quality of life of nurses. Some complementary interventions may influence the improvement of the quality of life. This study aimed to determine the effect of Benson's relaxation technique on the operating room nurses’ quality of life.Methods In this clinical trial, 180 operating room nurses were randomly allocated to two groups: intervention (Benson's relaxation technique) and control (routine activity). The study was conducted from December 2021 to March 2022 in hospitals affiliated with Shiraz University of Medical Sciences. Benson's relaxation technique was performed twice daily by the intervention group for 8 weeks. Quality of life was measured before and after the intervention using the SF-36 questionnaire. Data were analyzed using SPSS version 21 software, with a significance level set at a P-value of < 0.05.Results After the intervention, the results showed that Benson's relaxation technique led to an increase in the quality of life in the intervention group (P-value < 0.0001). In terms of quality-of-life dimensions, significant improvements were observed in the intervention group in the domains of physical problems (P-value = 0.026), emotional problems (P-value = 0.010), general health (P-value < 0.038), pain (P-value = 0.0001), and social functioning (P-value = 0.001). However, no significant differences were found in the dimensions of physical functioning (P-value = 0.294), energy (P-value = 0.078), and emotional well-being (P-value = 0.087).Conclusions Benson's relaxation technique can serve as a complementary method to enhance various dimensions of the operating room nurses’ quality of life.Trial Registration Number: IRCT20211002052650N1 with the Clinical Trial Registry (26.10.2022)
Coupled Flow CEEMDAN-SSA-BiLSTM-based predictive model
Authors: Zhang Xianqi , Zheng Yupeng , Yang Yang , Liu Yike , Yan Kaiwei
Published: June 2024

Flow is one of the important hydrological elements to study the water ecology and water environment of rivers in nature. Predicting flow is crucial for gathering valuable research data to aid in flood prevention, mitigation efforts, and the sustainable harnessing and utilization of water resources in the basin. To enhance the accuracy of flow prediction, a novel approach has been proposed. This methodology integrates the Adaptive Noise Complete Ensemble Empirical Modal Decomposition (CEEMDAN) with a Long and Short-Term Memory (LSTM) model, further refined through the application of the Sparrow Search Algorithm (SSA). The result is a powerful and innovative Combined Runoff Prediction Model, referred to as CEEMDAN-SSA-BiLSTM. This integrated model aims to provide more reliable predictions for both long and short-term runoff scenarios, contributing to more effective water resource management and environmental preservation in the basin. The daily flow trends from 2016 to 2022 were analyzed at four hydrological stations, namely Huayuankou, Jiahetan, Gaocun, and Lijin. The overall process is to use 80% daily flow data trained to predict 20% daily flow. Combined with the evaluation indexes used, the final series of results obtained are compared with the prediction results of several models, such as LSTM, BiLSTM, and CEEMDAN-BiLSTM, in multiple ways. The ultimate comparative outcomes demonstrate that the CEEMDAN-SSA-BiLSTM coupling exhibits a notable level of accuracy in forecasting daily flow. It has less error compared to several other models.
The effect of climatic conditions on the seasonality of pest insect populations in Eucalyptus plantations in the Neotropics
Authors: Costa Erika Carla Moraes da , Silva Daniel Paiva , Lodi Sara , Simões Daniel Albeny , Godoy Bruno Spacek
Published: June 2024

Eucalyptus (Eucalyptus spp., Mirtaceae) stands out for its remarkable regeneration capacity, making it a valuable tool for recovering degraded areas and reforestation. Despite its origins in Australia, eucalyptus has shown remarkable adaptation to the Brazilian tropical climate, contributing significantly to the wood productivity of the country. However, the extensive monoculture of eucalyptus can increase the incidence of pests, representing a significant challenge for the forestry sector, with production losses of up to 30%. In our study, we analyzed the occurrence of 11 pest insect species in 450 eucalyptus stands over nine years in the southeastern region of Brazil. We related pest occurrences to specific year periods and climatic variations using circular uniformity analysis and zero-inflated models. We found that the species generally have isolated peaks of occurrence during the year and that the increase in the occurrence of at least five species was related to temperature or rainfall. The results highlight the sensitivity of pests to climatic conditions, showing the importance of management strategies to ensure the sustainability and productivity of eucalyptus plantations. We can develop more effective management strategies by understanding how these variables interact with insect populations, contributing to the sustainability and productivity of eucalyptus plantations with less aggressive environmental practices.
Contribution of Simulation-Based Learning in Advanced Cardiopulmonary Resuscitation for Undergraduate Medical Students
Authors: Laajimi Sondes , Bradai Haifa , Mbarek Rabeb , Chebbi Nabil , Mastouri Omar , Chebili Naoufel , Loghmari Dorra , Naija Walid
Published: June 2024

Background
Healthcare simulation has become a crucial pedagogical technique across various medical disciplines.This approach use, virtual reality, or standardized patients to replicate clinical scenarios, has proven vital in preparing medical students for high-risk situations, ensuring the safety and effectiveness of critical patient management through the acquisition of technical skills, teamwork, and the ability to handle exceptional scenarios. Aims of this study wereto evaluate the theoretical knowledge of students before and after the simulation-based training (SBT) of the "Advancedcardiopulmonary resuscitation(CPR)" module, to assess their technical and non-technical skills and to describe their satisfaction.
Methods
This study, conducted at the Faculty of Medicine in Sousse, Tunisia, sought to objectively evaluate the impact of SBT on the theoretical knowledge, technical skills, and non-technical competencies of graduate Medical Students, during their advanced (CPR) training. The researchers employed a pre-experimental design, assessing students before and after the simulation-based workshops covering various CPR-related topics.
Results
We have demonstrated that the SBT was highly valued by the learners and led to significant improvements in their theoretical knowledge,There is a positive correlation between the pre and post-test score, with a correlation coefficient of r = 0.474, p < 0.0001, r² = 0.245. For technical skills, (69.8%) have improving their external cardiac massage (ECM) technique by the exam day, while 14 (16.3%) exhibited a decline (p < 0.001).Additionally, "non-technical skills"( NTS) were assessed using the Anesthesia Non-Technical Skills(ANTS) scoreamong these students, 85 (89.5%) improved their scores, while 10 (10.5%) maintained their initial score (p < 0.001).At the end of the training sessions, a strong statistically significant correlation was found between the sum of post-test scores, ANTS score, cardiac massage score, and the final score of the simulated practical “ECOS” exam (r = 0.762, p < 0.001, r²=0.581).Student satisfaction was evaluated; overall, students' impressions were predominantly excellent in over 50% of responses.
Conclusion
The study's contribution to the growing body of evidence supporting the integration of simulation-based learning in the formative years of medical education is particularly noteworthy. Simulation can accelerate skill acquisition and enhance the transition of knowledge and self-confidence when faced with real-life critical scenarios.
Investigation of genes involved in scent and color production in Rosa Damascena Mill
Authors: Kiani Hoda Sadat , Noudehi Manijeh Sabokdast , Shokrpour Majid , Zargar Meisam , Naghavi Mohammad Reza
Published: June 2024

Rosa damascena Mill., commonly known as the King Flower, is a fragrant and important species of the Rosaceae family. It is widely used in the perfumery and pharmaceutical industries. The scent and color of the flowers are significant characteristics of this ornamental plant. This study aimed to investigate the relative expression of MYB1, CCD1, FLS, PAL, CER1, GT1, ANS and PAR genes under two growth stages (S1 and S2) in two accessions. The CCD1 gene pathway is highly correlated with the biosynthesis of volatile compounds. The results showed that the overexpression of MYB1, one of the important transcription factors in the production of fragrance and color, in the pink accession of sample S2 increased the expression of PAR, PAL, FLS, RhGT1, CCD1, ANS, CER1, and GGPPS. The methyl jasmonate stimulant had a positive and cumulative effect on gene expression in most genes, such as FLS in ACC.26 of the S2 sample, RhGT1, MYB1, CCD1, PAR, ANS, CER1, and PALin ACC.1. To further study, a comprehensive analysis was performed to evaluate the relationship between the principal volatile compounds and colors. Our data suggest that the rose with pink flowers had a higher accumulation content of flavonoids and anthocyanin. To separate essential oil compounds, GC/MS analysis identified 26 compounds in four samples. The highest amount of geraniol, one of the main components of damask rose, was found in the dark-pink flower, 23.54%, under the influence of the methyl jasmonate hormone.
Development of an Ultrasound-Based Clinical Decision Rule to Rule-out Diverticulitis
Authors: Selame Lauren Ann , Shokoohi Hamid , Loesche Michael
Published: June 2024

The concern for diverticulitis often leads to the use of computed tomography (CT) scans for diagnosis. We aim to develop an ultrasound-based clinical decision rule (CDR) to confidently rule-out the disease without requiring a CT scan. We analyzed data from a prospective study of adult emergency department (ED) patients with suspected diverticulitis who underwent both bedside ultrasound (US) and CT. Patient history, physical examination, laboratory findings, and US results were used to create a CDR via a recursive partitioning model designed to prioritize sensitivity, with a loss matrix that heavily penalized false negatives. We calculated test characteristics for the CDR (TICS-Rule) and assessed the potential reduction in CT scans and ED length of stay. Data from 149 patients (84 female; mean age 58 ± 16) were used to develop the TICS-Rule. The final model integrates US diagnosis of simple and complicated diverticulitis with variables of heart rate, age, history of diverticulosis, vomiting, and leukocytosis. Negative US findings and a heart rate below 100 effectively excluded diverticulitis. The sensitivity increased from 54.5% (32.2–75.6) in the US alone to 100% (84.6–100%) for complicated diverticulitis in the model. The TICS-Rule missed no cases of complicated diverticulitis but one case of simple diverticulitis. The median time from ED greeting to US interpretation was 103 minutes (IQR 62–169), compared to 285 minutes (IQR 229–372) for CT. The TICS-Rule, developed using patient history, labs, and US findings, effectively excludes diverticulitis without the need for CT scans. With high sensitivity and reduced time compared to CT, it offers a promising approach to enhancing clinical decision-making, leading to CT and ED length of stay reductions.
Associations among authoritative leadership, organizational climate, job embeddedness and voice behavior in clinical nurses: a cross-sectional study in China
Authors: Luo Yixin , Chen Jianing , Zhang Yiqiu , Lu Guangli , Chen Chaoran
Published: June 2024

Introduction: The voice behavior of clinical nurses significantly influences the development of the healthcare industry. Understanding the factors influencing clinical nurses' voice behavior can help enhance the effectiveness of leadership styles across different cultures, promote a positive organizational climate, and increase employees' sense of job embeddedness, thereby encouraging more voice behavior. Therefore, this study examines the relationships between authoritative leadership, organizational climate, job embeddedness, and voice behavior.
Aim: The aim of this study was to examine the chain mediating effects of organizational climate and job embeddedness on the relationship between authoritativeleadership and clinical nurses’ voice behavior in China.
Methods: A cross-sectional survey study. We employed a convenient sampling method to recruit 809 clinical nurses from 5 comprehensive hospitals in Henan Province, China from October to November 2023.The scale questionnaires were used to assess, SPSS 25.0 was used for data analysis, and AMOS 26.0 software was used to examine the relationship between variables.
Results: The results showed that authoritative leadership and voice behavior were significantly negatively correlated (r = - 0.18, P＜0.01), and organizational climateand job embeddedness were positively correlated with voice behavior (r = 0.27, P＜0.01; r = 0.40, P＜0.01). Organizational climate and job embeddedness played a chain mediating role in the relationship between authoritative leadership and voice behavior of clinical nurses, accounting for 31.41% of the total effect.
Conclusions: Authoritative leadership has a negative impact on the clinical nurses’ voice behavior. More voice behavior may be taken by choosing appropriate leadership styles, such as recognizing potential drawbacks of authoritative leadership. Meanwhile, organizational climate and job embeddedness acted as a chain mediator between authoritative leadership and clinical nurses’ voice behavior. Nursing managers should focus on developing a positive organizational climate, enhancing employees' sense of job embeddedness, and promoting positive voice behavior among clinical nurses.
Blue Carbon in a Sub-Antarctic Marine Protected Area: Current and Future Perspectives
Authors: Bergagna Lucía , Lovrich Gustavo , Riccialdelli Luciana , Sahade Ricardo
Published: June 2024

Carbon fixation, storage, and eventual sequestration by marine ecosystems are known as “blue carbon”. This carbon uptake by the oceanic biological pump reduces atmospheric carbon dioxide (CO2) and is a major negative feedback mechanism to climate change. Benthic assemblages and their related Nature Contributions to People in Namuncurá – Burdwood Bank I and II (BB), two offshore sub-Antarctic Marine Protected Areas (MPAs), are the conservation values of these MPAs. Here, we show that the C reservoirs of these MPAs can be greater than those of their Antarctic counterparts, which, together with their extension, highlights their relevance. Organic and inorganic carbon were measured in the sediments and macrozoobenthic assemblages. More carbon was stored in the sediments than in the macrobenthic organisms, and the inorganic fraction largely exceeded the organic fraction. Most carbon assessments have focused only on the organic fraction, probably due to the complexity of processes involved in CaCO3 deposition, which starts releasing CO2. We compare various approaches for incorporating carbonates into carbon estimation and underscore the need to consider them because of their high abundance. Additionally, this work highlights the importance of sub-Antarctic benthic ecosystems as nature-based solutions to climate change.
Home-based urinary HPV self-sampling for the detection of cervical cancer precursor lesions: attitudes and preferences from Belgian females participating in the CASUS study
Authors: Hendrickx Jhana O. , Keer Severien Van , Donders Gilbert , Weyers Steven , Doyen Jean , Beyers Koen C.L. , Rios-Cortes Alejandra , Meers Nette , Téblick Laura , Vankerckhoven Vanessa V.J.
Published: June 2024

Background
Cervical cancer (CC) is the fourth most common cancer globally in females, caused by oncogenic infections with high-risk human papillomavirus (hrHPV) strains. Successful CC screening programs strongly depend on the participation rate of the target populationNevertheless, it remains challenging to reach under screened populations, including those with an increased CC risk. The CASUS study aimed to develop a complete CC screening solution based on first-void urine (FVU) self-sampling. Here we report on the usability perceptions and preferences from females that participated in the CASUS study by collecting FVU, also referred to as first-catch urine, as a liquid biopsy.
Methods
Females self-collected FVU samples at home the day before colposcopy using the Colli-Pee® UCM FV-5010, a FVU collection device prefilled with 3 mL of UCM preservative, collecting a total volume of 10mL. Afterwards, they completed a questionnaire expressing their usability perceptions and preferences regarding the device.
Results
A total of 332 females (26-70y) were enrolled in the CASUS study of which 210 completed the questionnaire. Overall, 66.6% of females preferred FVU self-sampling over a physician taken cervical sample (PTS) (32.9%) for their next CC screening. Out of 159 women who reported prior experience with a urine cup, 79.2% () expressed a preference for using the Colli-Pee® UCM FV-5010, while 20.8% favored the traditional urine cup. Additionally, 96.6% () of females found Colli-Pee® UCM FV-5010 easy to use and 97.1% would use the device again. A total of 208 valid System Usability Score (SUS) scores were received with an average of 86.17 ± 1.03 Standard Error of Mean (SEM).
Conclusion
The results of this study show that the majority of females in this referral cohort would prefer to self-collect a FVU sample at-home over a PTS for their next CC screening. Moreover, Colli-Pee® UCM FV-5010 was considered an easy-to-use and well-accepted self-sampling device for CC screening in a Belgian colposcopy referral population. From a future perspective, these results highlight the possibility of home-based FVU self-sampling as a liquid biopsy in CC screening where under screened populations could be approached more easily.
Trial registration:
The CASUS study was registered in ClinicalTrials.gov (identifier: NCT04530201).
Spike-in enhanced phosphoproteomics uncovers synergistic signaling responses to MEK inhibition in colon cancer cells
Authors: Selbach Matthias , Bentum Mirjam van , Klinger Bertram , Sieber Anja , Lehmann Nadine , Haji Mohamed , Naghiloo Sheyda , Niquet Sylvia , Mertins Philipp , Blüthgen Nils
Published: June 2024

Targeted kinase inhibitors are a cornerstone of cancer therapy, but their success is often hindered by the complexity of cellular signaling networks that can lead to resistance. Overcoming this challenge necessitates a deep understanding of cellular signaling responses. While standard global phosphoproteomics offers extensive insights, lengthy processing times, the complexity of data interpretation, and frequent omission of crucial phosphorylation sites limit its utility. Here, we combine data-independent acquisition (DIA) with spike-in of synthetic heavy stable isotope-labeled phosphopeptides to facilitate the targeted detection of particularly informative phosphorylation sites. Our spike-in enhanced detection in DIA (SPIED-DIA) approach integrates the improved sensitivity of spike-in-based targeted detection with the discovery potential of global phosphoproteomics into a simple workflow. We employed this method to investigate synergistic signaling responses in colorectal cancer cell lines following MEK inhibition. Our findings highlight that combining MEK inhibition with growth factor stimulation synergistically activates JNK signaling in HCT116 cells. This synergy emphasizes the therapeutic potential of concurrently targeting MEK and JNK pathways, as evidenced by the significantly impaired growth of HCT116 cells when treated with both inhibitors. Our results demonstrate that SPIED-DIA effectively identifies synergistic signaling responses in colorectal cancer cells, presenting a valuable tool for uncovering new therapeutic targets and strategies in cancer treatment.