bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Kinematic Sequences During Curveball and Fastball Baseball Pitches
Authors: Scarborough Donna Moxley , Colón Pablo E. , Linderman Shannon E. , Berkson Eric M.
Published: September 2021

Performance of a sequential proximal-to-distal transfer of segmental angular velocity (or Kinematic Sequence) is reported to reduce stress on musculoskeletal structures and thus the probability of injury while also maximizing ball velocity. However, there is limited investigation regarding the Kinematic Sequence of the five body segments (Pelvis, Trunk, Arm, Forearm, and Hand) among baseball pitchers. Some biomechanical and epidemiology studies have reported an association of the curveball with increased risk for elbow injury among youth pitchers. Kinematic Sequences with altered distal upper extremity (forearm and hand) sequences have been associated with greater elbow valgus and shoulder external rotation torques compared to other Kinematic Sequences. Identifying Kinematic Sequence patterns during curveball pitches may lead to improved understanding of injury susceptibility. This study investigated the Kinematic Sequence patterns (and their variability) during curveball pitching and compared them to the sequences identified during fastball pitches. Using 3D motion analyses, 14 baseball pitchers (four high school, eight college, and two professional) performed 5–6 curveball pitches and 12 pitchers also threw fastball pitches in a simulated bullpen session. Eleven different curveball Kinematic Sequences were identified and 8 fastball Kinematic Sequences. There was no significant variability in the number of Kinematic Sequences performed between the two pitch types, (Z= −0.431,p= 0.67). The median number of KSs performed by each group was 2.5. The most frequently used Kinematic Sequences for both pitch types were due to alteration in the sequence of the distal segments. The total percentage of Kinematic Sequences with altered distal segment sequencing for the curveball pitches was 49% and 43% for fastball pitches. Identifying the frequency of Kinematic Sequences with altered timing of hand and forearm peak velocities across pitch types may lead to a better understanding of the stresses that individual pitchers incur.
Fully Automatic Operation Algorithm of Urban Rail Train Based on RBFNN Position Output Constrained Robust Adaptive Control
Authors: Yang Junxia , Zhang Youpeng , Jin Yuxiang
Published: September 2021

High parking accuracy, comfort and stability, and fast response speed are important indicators to measure the control performance of a fully automatic operation system. In this paper, aiming at the problem of low accuracy of the fully automatic operation control of urban rail trains, a radial basis function neural network position output-constrained robust adaptive control algorithm based on train operation curve tracking is proposed. Firstly, on the basis of the mechanism of motion mechanics, the nonlinear dynamic model of train motion is established. Then, RBFNN is used to adaptively approximate and compensate for the additional resistance and unknown interference of the train model, and the basic resistance parameter adaptive mechanism is introduced to enhance the anti-interference ability and adaptability of the control system. Lastly, on the basis of the RBFNN position output-constrained robust adaptive control technology, the train can track the desired operation curve, thereby achieving the smooth operation between stations and accurate stopping. The simulation results show that the position output-constrained robust adaptive control algorithm based on RBFNN has good robustness and adaptability. In the case of system parameter uncertainty and external disturbance, the control system can ensure high-precision control and improve the ride comfort.
Optimal Design of Magneto-Force-Thermal Parameters for Electromagnetic Actuators with Halbach Array
Authors: Li Zhihao , Wu Qianqian , Liu Bilong , Gong Zhaopei
Published: September 2021

A magnetic levitation isolation system applied for the active control of micro-vibration in space requires actuators with high accuracy, linear thrust and low power consumption. The magneto-force-thermal characteristics of traditional electromagnetic actuators are not optimal, while actuators with a Halbach array can converge magnetic induction lines and enhance the unilateral magnetic field. To improve the control effect, an accurate magnetic field analytical model is required. In this paper, a magnetic field analytical model of a non-equal-size Halbach array was established based on the equivalent magnetic charge method and the field strength superposition principle. Comparisons were conducted between numerical simulations and analytical results of the proposed model. The relationship between the magnetic flux density at the air gap and the size parameters of the Halbach array was analyzed by means of a finite element calculation. The mirror image method was adopted to consider the influence of the ferromagnetic boundary on the magnetic flux density. Finally, a parametric model of the non-equal-size Halbach actuator was established, and the multi-objective optimization design was carried out using a genetic algorithm. The actuator with optimized parameters was manufactured and experiments were conducted to verify the proposed analytical model. The difference between the experimental results and the analytical results is only 5%, which verifies the correctness of the magnetic field analytical model of the non-equal-size Halbach actuator.
The Impact of Steady Blowing from the Leading Edge of an Open Cavity Flow
Authors: Haddabi Naser Al , Kontis Konstantinos , Zare-Behtash Hossein
Published: September 2021

Cavity flows occur in a wide range of low-speed applications (Mach number ≤0.3), such as aircraft wheel wells, ground transportation, and pipelines. In the current study, a steady jet is forced from a cavity leading edge at different momentum fluxes (0.11 kg/ms2, 0.44 kg/m·s2, and 0.96 kg/m·s2). The investigation was performed for an open cavity with length to depth ratio of 4 at the Reynolds number based on a cavity depth of approximately 50,000. Particle image velocimetry, surface oil flow visualisation, constant temperature anemometry, and pressure measurements were performed in this investigation. The aim of the jet blowing is to separate the cavity separated shear layer from the recirculation zone to reduce the cavity return flow, and hence stabilise the cavity separated shear layer. It was found that increasing the jet momentum flux causes an increase in the cavity return flow due to the increase in the thickness of the cavity separated shear layer. The study also found that the jet populates the separated shear layer with a large number of small-scale disturbances. These disturbances increase the broad band level of the pressure power spectra and Reynolds shear stress in the cavity separated shear layer. On the other hand, the jet disturbances make the shedding of the large vortical structures more intermittent.
Evaluation and Comparison of Hybrid Wing VTOL UAV with Four Different Electric Propulsion Systems
Authors: Zong Jianan , Zhu Bingjie , Hou Zhongxi , Yang Xixiang , Zhai Jiaqi
Published: September 2021

Electric propulsion technology has attracted much attention in the aviation industry at present. It has the advantages of environmental protection, safety, low noise, and high design freedom. An important research branch of electric propulsion aircraft is electric vertical takeoff and landing (VTOL) aircraft, which is expected to play an important role in urban traffic in the future. Limited by battery energy density, all electric unmanned aerial vehicles (UAVs) are unable to meet the longer voyage. Series/parallel hybrid-electric propulsion and turboelectric propulsion are considered to be applied to VTOL UAVs to improve performances. In this paper, the potential of these four configurations of electric propulsion systems for small VTOL UAVs are evaluated and compared. The main purpose is to analyze the maximum takeoff mass and fuel consumption of VTOL UAVs with different propulsion systems that meet the same performance requirements and designed mission profiles. The differences and advantages of the four types propulsion VTOL UAV in the maximum takeoff mass and fuel consumption are obtained, which provides a basis for the design and configuration selection of VTOL UAV propulsion system.
Alternative Models for Calculation of Static Overturning Angle and Lateral Stability Analysis of Subcompact and Universal Tractors
Authors: Majdan Radoslav , Abrahám Rudolf , Kollárová Katarína , Tkáč Zdenko , Matejková Eva , Kubík Ľubomír
Published: September 2021

Vehicle lateral stability is evaluated using the static overturning angle. The correct value of this parameter depends on the calculation method. The aim of this study was to compare the latest standard with previously published methodology, to propose two alternative methodologies (Models 1 and 2) and to analyze the influence of various levels of rear wheel ballast weights and overall tire widths on the stability of universal and subcompact tractors. The results showed a significant regression effect of the rear wheel ballast weight on static overturning angle. The influence of the rear wheel ballast weight was higher in the subcompact tractor than in the universal tractor due to a larger distance between the height of the center of gravity and the center of the rear axle. Comparing the latest standard with the previously published methodology, the highest difference values were 13.82% and 7.30%. Both models are based on the previously published methodology and differ from each other in rolling and slope lines. The methodology proposed in Model 2 differed from the standard similarly to the previously published methodology; therefore, it is irrelevant. Model 1 reached differences of only −1.81% and −1.63%, representing a minimal difference from the standard.
COVID-19 Effects and Resilience of Vegetable Farmers in North-Western Nigeria
Authors: Yegbemey Rosaine N. , Komlan Ahihou Christelle M. , Olorunnipa Ifeoluwa , Benali Marwan , Afari-Sefa Victor , Schreinemachers Pepijn
Published: September 2021

The COVID-19 pandemic has particularly affected the supply of perishable foods such as vegetables, which could adversely affect food and nutrition security. Here, we study the mechanisms by which COVID-19 has affected vegetable production and the coping strategies adopted by smallholder farmers. We use cross-sectional data collected through individual interviews on a random sample of 521 vegetable producers in north-western Nigeria. The perceptions of respondents, measured on a 5-point Likert scale (from 1—not affected, to 5—severely affected), shows that COVID-19 had an average effect of 3.07 (±1.23) on vegetable production. Farmers also reported challenges in accessing farm inputs and storing or selling fresh vegetable produced. In response, farmers reduced market-oriented vegetable production, produced more vegetables for own consumption, added value through own home processing and storage, explored new markets, and accepted lower farmgate selling prices. A multivariate probit regression shows that socio-economic factors such as age, household size, marital status, challenges in accessing inputs, and perceptions of the effects of COVID-19 influenced farmers’ decisions to adopt particular coping strategies. To sustain vegetable supplies, policy makers should consider investing more in market-oriented strategies such as vegetable processing and storage, which individual farmers may not be able to afford due to high costs, lack of information and required knowledge on good agronomic practices, postharvest handling, storage and market. Public extension services can contribute to help farmers to adapt better.
Does the Level of Financial Cognition Affect the Income of Rural Households? Based on the Moderating Effect of the Digital Financial Inclusion Index
Authors: Zou Fanqi , Li Tinghui , Zhou Feite
Published: September 2021

Financial cognitive ability is an important part of human capital, which has a direct impact on household income. The dual differences composed by rural residents’ cognition level and residents’ income make the relationship between the two complicated. Based on the analysis of the impact of financial cognitive level on rural household income, this paper takes into account the individual heterogeneity of financial cognition to build an unconditional quantile regression (UQR) model through the micro data of the China Household Financial Survey Project (CHFS). Incorporating the background of the rapid development of digital finance, this paper also studies the impact of rural residents’ financial cognition on household income. The empirical results of the article have the following conclusions: First, on the whole, the improvement of financial cognitive level promotes the increase of rural household income, but this promotion has significant differences at different quantile levels of household income. Second, the digital financial inclusion index in the region has a positive moderating effect on the impact of financial cognition on the income of rural households. Third, the heterogeneity of the impact of financial cognition on the income of rural households is reflected in the two aspects of regions and household assets.
Physicochemical and Sensory Attributes of Intact and Restructured Chicken Breast Meat Supplemented with Transglutaminase
Authors: Kaić Ana , Janječić Zlatko , Žgur Silvester , Šikić Monika , Potočnik Klemen
Published: September 2021

Transglutaminases (TG) are enzymes that improve the functional properties of proteins in meat products, contribute to the strong cohesion of meat without the further need for the addition of sodium chloride or phosphates, and have a positive effect on the texture of the meat product. This study aimed to investigate the physicochemical and sensory attributes of intact and restructured chicken meat supplemented with different TG proportions. The study was conducted on chicken breast meat samples (n = 40) originating from the line Ross 308. The intact samples were separated from the pectoralis major muscle, whereas the rest of the breast meat was ground, divided into equal parts, and supplemented with TG (0.2%; 0.4%; 0.6%; 0.8%; 1%). The intact meat had the highest cooking loss (19.84) when compared to 0.2% (15.51), 0.4% (15.04), 0.6% (14.95), 0.8% (14.95), and 1% (15.79) TG-supplemented meat. The intact meat had greater shear force (16.90) than 0.2% (5.16), 0.4% (5.39), 0.6% (5.16), 0.8% (5.98), and 1% (6.92) TG supplemented meat. There was no difference between intact meat and TG-supplemented meat in color, taste, odor, texture, and overall acceptability (p > 0.05). Therefore, TG supplementation can be used in improving yield and texture of minced chicken meat.
Effect of Increased Salt Water Intake on the Production and Composition of Dairy Goat Milk
Authors: Costa Roberto Germano , Freire Rayssa M. Bezerril , de Araújo Gherman Garcia Leal , Queiroga Rita de Cássia Ramos do Egypto , Paiva Gutemberg Nascimento , Ribeiro Neila Lidiany , Oliveira Ronaldo Lopes , Domínguez Rubén , Lorenzo José M.
Published: September 2021

Due to its necessity and magnitude, water is essential for animal nutrition. This study aimed to evaluate the effects of increasing levels of water salinity on the quality of goat milk in the Brazilian semiarid region. Twenty-four multiparous Alpine goats, with an average live weight of 38.0 ± 4.0 kg and an average lactation period of 30 days, distributed entirely at random, were used. The experiment lasted 64 days including an initial period of 14 days of adaption to the diet. The experimental treatments consisted of water with different levels of total dissolved solids (TDS): 640, 3188, 5740, and 8326 mg L−1, obtained using sodium chloride (NaCl). Increasing the levels of TDS in drinking water from 640 to 8326 mg L−1 did not significantly (p > 0.05) affect the production and the physicochemical composition of the milk. There was a linear increase (p < 0.05) in the water consumption and acidity variables as a function of the total dissolved solid levels. The mineral composition of the milk was not altered with increasing levels of TDS in water from 640 to 8326 mg L−1. There was no negative effect (p > 0.05) for any of the sensorial attributes analyzed in relation to the treatments. Therefore, as a general conclusion, based on the analyses carried out in this experiment, it was found that water with total dissolved solids, when supplied for short periods of up to 48 days, does not alter the production, physicochemical characteristics, or the organoleptic properties of goat’s milk.
Effect of Yellow Wine Lees Supplementation on Milk Antioxidant Capacity and Hematological Parameters in Lactating Cows under Heat Stress
Authors: Yao Kaiyong , Jiang Luyi , Liu Jianxin , Wang Diming , Liu Hongyun , Ren Daxi
Published: September 2021

Fifteen multiparous lactating Chinese Holstein dairy cows were used in a replicated 3 × 3 Latin Square Design to evaluate the effect of total mixed rations (TMR) containing unfermented and fermented yellow wine lees (YWL) on the oxidative status of heat-stressed lactating cows and the oxidative stability of the milk and milk fatty acids they produced. Cows were fed with three isonitrogenous and isocaloric diets as follows: (1) TMR containing 18% soybean meal, (2) TMR containing 11% unfermented YWL (UM), and (3) TMR containing 11% fermented YWL (FM). The rectal temperature (at 1300 h) and respiratory rate were higher in control cows than in cows fed UM or FM. Both types of YWL were greater in total phenolic and flavonoid contents, reducing power, and radical scavenging abilities than soybean meal. Cows fed UM or FM had higher blood neutrophil, white blood cell, and lymphocyte counts, as well as lower plasma malondialdehyde level, higher plasma superoxide dismutase, glutathione peroxidase, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate levels, and higher total antioxidant capacity in the plasma than those fed control diet. The proportion of milk unsaturated fatty acids was higher and that of saturated fatty acids was lower in UM- and FM-fed animals than in the control animals. Milk from UM- and FM-fed cows had lower malondialdehyde content but higher 2,2-diphenyl-1-picryl-hydrazyl-hydrate content than the control cows. In conclusion, feeding TMR containing UM and FM to cows reduced both the oxidative stress in heat-stressed cows and improved the oxidative capacity of their milk.
Shift in Circulating Serum Protein Fraction (SPF) Levels of Pregnant Jennies and Nutritional Related Aspects at Early-, Mid- and Late Gestation
Authors: Cappai Maria Grazia , Wolf Petra , Liesegang Annette , Biggio Giovanni Paolo , Podda Andrea , Varcasia Antonio , Tamponi Claudia , Berlinguer Fiammetta , Cossu Ignazio , Pinna Walter , Cherchi Raffaele
Published: September 2021

A viable tool for the monitoring of the systemic condition of the pregnant jenny may be the determination of serum protein fraction (SPF) levels, including metabolic profiling. Tissue development and composition of the growing fetus requires the mother to provide adequate nutrients to its body parts and organs. In this regard, body fluid distribution and strategic molecule transportation can be screened using SPF electropherograms and analysis of intermediate metabolites. The nutritional and health status of 12 jennies (age: 5–8 years; BW at the start: 135–138 kg; Body Condition Score, BCS [1 to 5 points] = 2.25–2.50; 4th month of gestation) were monitored throughout gestation (approximate gestation period 350–356 d). All animals were pasture-fed and were offered hay ad libitum. Individual blood samples were collected within the 4th, 7th, and 10th month following conception (ultrasound scanning). Serum biochemistry, in particular, the analysis of 6 fractions of serum proteins was carried out. The significant decrease in circulating albumin in jennies from mid- to late-gestation (p < 0.001) suggests a considerable role of dietary amino acids in the synthesis of protein for fetal tissue formation as well as body fluid distribution and blood pressure control of the jenny in those stages. Moreover, α1-globulin decreased significantly in late gestation (p < 0.047), corresponding to major organ development in the terminal fetus and supported by lipid transportation in the bloodstream of the jenny. Similarly, α2-globulin decreased in late gestation (p < 0.054) as haptoglobin, an important component for the transport of free circulating hemoglobin, is likely used for fetal synthesis. Mid-gestation, appears to be a crucial moment for adequate dietary nutrient supplementation in order to prevent homeostasis perturbation of jennies, as observed in this trial.
Insects as Novel Ruminant Feed and a Potential Mitigation Strategy for Methane Emissions
Authors: Ahmed Eslam , Fukuma Naoki , Hanada Masaaki , Nishida Takehiro
Published: September 2021

This study is the first to evaluate the chemical composition and impacts of four different edible insects, Acheta domesticus (A.d), Brachytrupes portentosus (B.p), Gryllus bimaculatus (G.b), and Bombyx mori (B.m), on the digestibility, rumen fermentation, and methane production when used as a substitute for 25% of the soybean meal (SBM) in a ruminant diet through in vitro incubation. The dietary treatments were 100% grass hay, 60% grass hay + 40% SBM, 60% grass hay + 30% SBM + 10% A.d, 60% grass hay + 30% SBM + 10% B.p, 60% grass hay + 30% SBM + 10% G.b, and 60% grass hay + 30% SBM + 10% B.m. The experiment was conducted as a short-term batch culture for 24 h at 39 °C, and the incubation was repeated in 3 consecutive runs. Chemical analysis of the insects showed that they were rich in fat (14–26%) with a high proportion of unsaturated fatty acids (60–70%). Additionally, the insects were rich in protein (48–61%) containing all essential amino acids and the amino acid profiles of the insects were almost the same as that of SBM. The inclusion of insects did not affect nutrient digestibility or the production of volatile fatty acids but did increase the production of ammonia-nitrogen. The addition of G.b and B.m led to decrease in methane production by up to 18% and 16%, respectively. These results reveal that substitution of 25% SBM in the diet with the tested insects had no negative impacts, and their potential to reduce methane production is an environmental benefit.
Assessment of Influential Factors for Scours Associated with Cryptosporidium sp., Rotavirus and Coronavirus in Calves from Argentinean Dairy Farms
Authors: Bertoni Emiliano , Barragán Adrián A. , Bok Marina , Vega Celina , Martínez Marcela , Gil José F. , Cimino Rubén O. , Parreño Viviana
Published: September 2021

Scours is the most common disease in dairy calves, and it is a multifactorial syndrome complex. Cryptosporidium sp. (C. sp.), rotavirus group A (RVA), and bovine coronavirus (BCoV) are the three main pathogens associated with scours. The objective of this study was to identify potential factors associated with scours, C. sp., RVA, and BCoV infections in preweaned dairy calves from Lerma Valley in Salta Province, Argentina. A total of 488 preweaned calves from 19 dairy farms located in the Lerma Valley were enrolled in this observational study. One fecal sample was collected from each calf between one week and two months of age for assessment of C. sp., RVA, and BCoV infection status. Cryptosporidium sp. oocysts and RVA and BCoV antigens in fecal samples were assessed using microscopic observation and indirect enzyme-linked immune sorbent assay (iELISA), respectively. A voluntary questionnaire was developed and used to collect data regarding management practices from the participants’ farms. The data were analyzed using multivariable logistic regression models. Scours incidence was 35.4%, and a greater proportion of calves younger than 20 days were affected. Of the fecal samples, 18% and 9.5% tested were positives for C. sp. and RVA, respectively, while BCoV was detected only in two calves. Furthermore, 84.2% and 63.1% of the farms tested positive for Cryptosporidium sp. and RVA, respectively. In addition, the following variables were associated with higher odds of having scours: (1) herd size (>300 milking cows; OR = 1.7), (2) calf age (<20 days of age; OR = 2.2), (3) RVA and C. sp. test (positive test; RVA OR = 2.6; C. sp. OR = 3), calf feeding practices (feeding milk replacer; OR = 1.81), and newborn calf management practices (calf moved from maternity pen <6 h after calving; OR = 1.7). Concerning RVA infection, calves less than 20 days of age (OR = 2.6) had a higher chance of testing positive for RVA, while calves that remained in the calving pen for less than 6 h after calving had a lower chance (OR = 0.3). On the other hand, for C. sp. infection, large farm size (>300 milking cows; OR = 1.2) and young calf age (<20 days of age; OR = 4.4) indicated a higher chance of testing positive for C. sp., while calves belonging to farms that fed frozen colostrum (OR = 0.2) had a lower chance of becoming infected with C. sp. The result of this study indicated that scours is a prevalent disease in farms of the Lerma Valley, Salta, Argentina, and that RVA and C. sp. infections, along with specific farm management practices, might be important contributing factors that could increase the chance of NCS in dairy farms.
A Taxonomic Survey of Female Oviducal Glands in Chondrichthyes: A Comparative Overview of Microanatomy in the Two Reproductive Modes
Authors: Marongiu Martina Francesca , Porcu Cristina , Pascale Noemi , Bellodi Andrea , Cau Alessandro , Mulas Antonello , Pesci Paola , Porceddu Riccardo , Follesa Maria Cristina
Published: September 2021

Oviducal glands (OGs) are distinct expanded regions of the anterior portion of the oviduct, commonly found in chondrichthyans, which play a key role in the production of the egg in-vestments and in the female sperm storage (FSS). The FSS phenomenon has implications for understanding the reproductive ecology and management of exploited populations, but little information is available on its taxonomic extent. For the first time, mature OGs from three lecithotrophic oviparous and four yolk-sac viviparous species, all considered at risk from the fishing impacts in the central western Mediterranean Sea, were examined using light microscopy. The OG microanatomy, whose morphology is generally conserved in all species, shows differences within the two reproductive modalities. Oviparous species show a more developed baffle zone in respect to viviparous ones because of the production of different egg envelopes produced. Among oviparous species, Raja polystigma and Chimaera monstrosa show presence of sperm, but not sperm storage as observed, instead, in Galeus melastomus and in all the viviparous sharks, which preserve sperm inside of specialized structures in the terminal zone.
Characterization of the Variables Related to Reports of Death Due to Canine Bites in Scientific Articles during the Years 2013–2017: A Systematic Review
Authors: Barrios Carmen Luz , Aguirre-Olea Valentina , Bustos-López Carlos , Pérez-Vergara Sandra , Claros-Alegría Sandra
Published: September 2021

Canine bites are an important public health problem, with consequences such as physical injuries, psychological trauma, transmission of zoonoses, infections and they can even cause death. To avoid deaths caused by this type of bite, multiple factors related to this issue must be considered. The objective of this review was to search and analyze the indexed scientific literature on canine bites resulting in death, published during the period 2013–2017. A search was carried out in various databases of indexed literature, in Spanish and English. After selecting and excluding items using PRISMA, they were classified according to SIGN guidelines to filter out the level of evidence and potential biases. Thirty-three scientific articles were retrieved and analyzed. In most of these, victims between 50 and 64 years of age (28.6%, 8/28) were registered. Additionally, in most of the articles, only one animal participated (80%, 16/20). The highest number of events occurred in public spaces (58.5%, 7/12). In conclusion, most of the scientific articles that report incidents of dog bites resulting in death, provide details about the victims, their injuries and the treatment received; however, few articles provide background information on the context of the attack and the biting animal.
Learn to Train: Improving Training Data for a Neural Network to Detect Pecking Injuries in Turkeys
Authors: Volkmann Nina , Brünger Johannes , Stracke Jenny , Zelenka Claudius , Koch Reinhard , Kemper Nicole , Spindler Birgit
Published: September 2021

This study aimed to develop a camera-based system using artificial intelligence for automated detection of pecking injuries in turkeys. Videos were recorded and split into individual images for further processing. Using specifically developed software, the injuries visible on these images were marked by humans, and a neural network was trained with these annotations. Due to unacceptable agreement between the annotations of humans and the network, several work steps were initiated to improve the training data. First, a costly work step was used to create high-quality annotations (HQA) for which multiple observers evaluated already annotated injuries. Therefore, each labeled detection had to be validated by three observers before it was saved as “finished”, and for each image, all detections had to be verified three times. Then, a network was trained with these HQA to assist observers in annotating more data. Finally, the benefit of the work step generating HQA was tested, and it was shown that the value of the agreement between the annotations of humans and the network could be doubled. Although the system is not yet capable of ensuring adequate detection of pecking injuries, the study demonstrated the importance of such validation steps in order to obtain good training data.
Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling
Authors: Hernández-Hernández José Manuel , Martin Graeme B. , Becerril-Pérez Carlos Miguel , Pro-Martínez Arturo , Cortez-Romero César , Gallegos-Sánchez Jaime
Published: September 2021

This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (n = 8), where the lambs remained with their mothers; and (2) restricted suckling (n = 8), where the mothers suckled their lambs twice daily for 30 min. On Day 19 postpartum, the ewes were individually penned with ad libitum access to water and feed and given an indwelling catheter in each jugular vein. On Day 20, 4 mL of blood was sampled every 15 min from 08:00 to 20:00 h to determine LH pulse frequency. At 14:00 h, four ewes in each group received 120 μg of kisspeptin diluted in 3 mL of saline as a continuous infusion for 6 h; the remaining four ewes in each group received only saline. The interaction between kisspeptin and suckling type did not affect LH pulse frequency (p > 0.05). Before kisspeptin administration, pulse frequency was similar in all groups (1.50 ± 0.40 pulses per 6 h; p > 0.05). With the application of kisspeptin, pulse frequency increased to 3.50 ± 0.43 pulses per 6 h (p ≤ 0.014), so the concentration of LH (1.11 ± 0.14 ng mL−1) was greater in kisspeptin-treated ewes than in saline-treated ewes (0.724 ± 0.07 ng mL−1; p ≤ 0.040). The frequency of LH pulses was greater with restricted suckling than with continuous suckling (2.44 ± 0.29 versus 1.69 ± 0.29 pulses per 6 h; p ≤ 0.040). We conclude that intravenous application of kisspeptin increases the pulsatile secretion of LH in suckling ewes and that suckling might reduce kisspeptin neuronal activity, perhaps explaining the suppression of ovulation. Moreover, the effects of kisspeptin and suckling on pulsatile LH secretion appear to be independent, perhaps operating through different neural pathways.
Extended or Continuous Infusion of Carbapenems in Children with Severe Infections: A Systematic Review and Narrative Synthesis
Authors: Zhou Pengxiang , Zhang Yahui , Wang Zhenhuan , Ying Yingqiu , Xing Yan , Tong Xiaomei , Zhai Suodi
Published: September 2021

We systematically reviewed the efficacy and safety of an extended or continuous infusion (EI/CI) versus short-term infusion (STI) of carbapenems in children with severe infections. Databases, including PubMed, Embase, the Cochrane Library, Clinicaltrials.gov, China National Knowledge Infrastructure, WanFang Data, and SinoMed, were systematically searched from their inceptions to 10 August 2020, for all types of studies (such as randomized controlled trials (RCTs), retrospective studies, and pharmacokinetic or population pharmacokinetic (PK/PPK) studies) comparing EI/CI versus STI in children with severe infection. There was no limitation on language, and a manual search was also conducted. The data were screened, evaluated, extracted, and reviewed by two researchers independently. Quantitative (meta-analysis) or qualitative analyses of the included studies were performed. Twenty studies (including two RCTs, one case series, six case reports, and 11 PK/PPK studies) were included in this review (CRD42020162845). The RCTs’ quality evaluation results revealed a risk of selection and concealment bias. Qualitative analysis of RCTs demonstrated that, compared with STI, an EI (3 to 4 h) of meropenem in late-onset neonatal sepsis could improve the clinical effectiveness and microbial clearance rates, and reduce the rates of mortality; however, the differences in the incidence of other adverse events were not statistically significant. Retrospective studies showed that children undergoing an EI of meropenem experienced satisfactory clinical improvement. In addition, the results of the PK/PPK study showed that an EI (3 or 4 h)/CI of carbapenems in severely infected children was associated with a more satisfactory goal achievement rate (probability of target attainment) and a cumulative fraction of response than STI therapy. In summary, the EI/CI of carbapenems in children with severe infection has a relatively sufficient PK or pharmacodynamic (PD) basis and satisfactory efficacy and safety. However, due to the limited quantity and quality of studies, the EI/CI therapy should not be used routinely in severely infected children. This conclusion should be further verified by more high-quality controlled clinical trials or observational studies based on PK/PD theories.
2-Oxo-Imidazole-Containing Dipeptides Play a Key Role in the Antioxidant Capacity of Imidazole-Containing Dipeptides
Authors: Kasamatsu Shingo , Komae Somei , Matsukura Kana , Kakihana Yuki , Uchida Koji , Ihara Hideshi
Published: September 2021

There is substantial evidence for the antioxidant functions of imidazole-containing dipeptides (IDPs), including carnosine and anserine, under physiological and pathological conditions in vivo. However, the detailed mechanism underlying the antioxidant functions is still poorly understood. Recently, we discovered the endogenous production of 2-oxo-imidazole-containing dipeptides (2-oxo-IDPs), such as 2-oxo-carnosine and 2-oxo-anserine, as novel derivatives of IDPs in mouse tissues and revealed that the antioxidant capacity of 2-oxo-carnosine was much greater than that of carnosine. However, the antioxidant capacity of 2-oxo-IDPs still remains unclear. In this study, we evaluated 2-oxo-carnosine and 2-oxo-anserine by multiple in vitro assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing/antioxidant power, and oxygen radical absorbance capacity assays in comparison with the corresponding IDPs, carnosine and anserine. All the assays employed herein demonstrated that 2-oxo-carnosine and 2-oxo-anserine exhibited a greater antioxidant capacity than that of the corresponding IDPs. Quantitative high-performance liquid chromatography tandem mass spectrometry revealed that commercial IDPs standards were contaminated with a certain amount of 2-oxo-IDPs, which was correlated with the antioxidant capacity. DPPH radical scavenging assay revealed that the elimination of contaminated 2-oxo-IDPs from the IDPs standards caused a significant decrease in the antioxidant capacity compared to the original IDPs standards. These results suggest that the main driver of the antioxidant capacity of IDPs is 2-oxo-IDPs; accordingly, the conversion of IDPs to 2-oxo-IDPs may be a critical step in the antioxidant functions.
Bromophenol Bis (2,3,6-Tribromo-4,5-dihydroxybenzyl) Ether Protects HaCaT Skin Cells from Oxidative Damage via Nrf2-Mediated Pathways
Authors: Dong Hui , Liu Mingfei , Wang Li , Liu Yankai , Lu Xuxiu , Stagos Dimitrios , Lin Xiukun , Liu Ming
Published: September 2021

Excessive reactive oxygen species (ROS) promotes the oxidative stress of keratinocytes, eventually causing cell damage. The natural bromophenol bis (2,3,6-tribromo-4,5-dihydroxybenzyl) ether (BTDE) from marine red algae has been reported to have a varied bioactivity; however, its antioxidant effect has yet to be investigated systemically. Our present work aimed to explore the antioxidant effect of BTDE both on the molecular and cellular models and also to illustrate the antioxidant mechanisms. Our results showed that BTDE could effectively scavenge ABTS free radicals and protect HaCaT cells from damage induced by H2O2. Mechanism studies in HaCaT cells demonstrated that BTDE attenuated hydrogen peroxide (H2O2)-induced ROS production, reduced the malondialdehyde (MDA) level, decreased the oxidized glutathione (GSSG)/glutathione (GSH) ratio, and increased the antioxidant enzyme superoxide dismutase (SOD). Moreover, BTDE could inhibit the expression of Kelch-like epichlorohydrin-associated protein 1 (Keap1) and increase the expression of both nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins TrXR1, HO-1, and NQO1. BTDE also activated the upstream signaling pathway of Nrf2 such as AKT pathway, while not activating the ERK or AMPKα pathways. In general, BTDE is a promising antioxidant to protect HaCaT cells against oxidative damage via Nrf2-mediated pathways.
Atomic Force Microscopy to Elicit Conformational Transitions of Ferredoxin-Dependent Flavin Thioredoxin Reductases
Authors: Marcuello Carlos , Frempong Gifty Animwaa , Balsera Mónica , Medina Milagros , Lostao Anabel
Published: September 2021

Flavin and redox-active disulfide domains of ferredoxin-dependent flavin thioredoxin reductase (FFTR) homodimers should pivot between flavin-oxidizing (FO) and flavin-reducing (FR) conformations during catalysis, but only FR conformations have been detected by X-ray diffraction and scattering techniques. Atomic force microscopy (AFM) is a single-molecule technique that allows the observation of individual biomolecules with sub-nm resolution in near-native conditions in real-time, providing sampling of molecular properties distributions and identification of existing subpopulations. Here, we show that AFM is suitable to evaluate FR and FO conformations. In agreement with imaging under oxidizing condition, only FR conformations are observed for Gloeobacter violaceus FFTR (GvFFTR) and isoform 2 of Clostridium acetobutylicum FFTR (CaFFTR2). Nonetheless, different relative dispositions of the redox-active disulfide and FAD-binding domains are detected for FR homodimers, indicating a dynamic disposition of disulfide domains regarding the central protein core in solution. This study also shows that AFM can detect morphological changes upon the interaction of FFTRs with their protein partners. In conclusion, this study paves way for using AFM to provide complementary insight into the FFTR catalytic cycle at pseudo-physiological conditions. However, future approaches for imaging of FO conformations will require technical developments with the capability of maintaining the FAD-reduced state within the protein during AFM scanning.
Simulation and Evaluation of the Radiation Dose Deposited in Human Tissues by Atmospheric Neutrons
Authors: Ortiz Ernesto , Mendoza Blanca , Gay Carlos , Mendoza Victor Manuel , Pazos Marni , Garduño Rene
Published: September 2021

The evaluation of the radiation dose (RD) deposited by atmospheric neutrons in human tissues is of vital importance due to the potential damages that over exposure to this radiation may cause to human health. The goal of this work was to obtain the RD that atmospheric neutrons with energy from 1 to 1000 MeV deposit in tissues of the human body (blood, adipose, bone and brain) as a function of both altitude and latitude. With the help of the Geant4 software, we developed a numerical simulation that allowed us to reach our goal; atmospheric neutron fluxes were obtained from the Excel-Based Program for Calculating Atmospheric Cosmic-Ray Spectrum (EXPACS). We found that the RD deposited by atmospheric neutrons increases with the increase in altitude and latitude, e.g., for an altitude of high mountain (4 km), the RD is increased ∼19 times; while, for an altitude of commercial flights (10 km), the RD is increased ∼156 times; in both cases, regarding the RD at sea level. We also found that, in the range of energies from 1 to 100 MeV, the RD deposited in the bone tissue sample is considerably lower that the RD deposited in the blood, adipose and brain tissue samples. On the other hand, for the range of energies between 200 and 1000 MeV, the RD deposited in the bone tissue sample is considerably greater that the RD deposited in the blood, adipose and brain tissue samples.
Special Issue on Image Simulation in Remote Sensing
Authors: Eo Yang Dam
Published: September 2021

Recently, various remote sensing sensors have been used and their performance has developed rapidly [...]
Special Issue “New Trends in Sport and Exercise Medicine”
Authors: Galli Daniela
Published: September 2021

The practice of regular physical activity has been proposed as a determinant in many disciplines, from wellness to physiotherapy; in fact, it reduces the risks of cardiovascular diseases and diabetes [...]
The Effect of Contrast Agents on Dose Calculations of Volumetric Modulated Arc Radiotherapy Plans for Critical Structures
Authors: Elawadi A. A. , AlMohsen Safa , AlGendy Reham , Allazkani Hosam , Mohamed Reham A. , AlAssaf Hossam , Nisbet Andrew , Alshanqity Mukhtar
Published: September 2021

Radiotherapy dose calculation requires accurate Computed Tomography (CT) imaging while tissue delineation may necessitate the use of contrast agents (CA). Acquiring these two sets is a common practice in radiotherapy. This study aims to evaluate the effect of CA on the dose calculations. Two hundred and twenty-six volumetric modulated arc therapy (VMAT) patients that had planning CT with contrast (CCT) and non-contrast CT (NCCT) of different cancer sites (e.g., brain, head, and neck (H&N), chest, abdomen, and pelvis) were evaluated. Treatment plans were recalculated using CCT, then compared to NCCT. The variation in Hounsfield units (HU) and dose distributions for critical structures and target volumes were analyzed using mean HU, mean and maximum relative dose values, D2%, D98%, and 3D gamma analysis. HU variations were statistically significant for most structures. However, this was not clinically significant as the difference in mean HU values was within 30 HU for soft tissue and 50 HU for lungs. Variation in target volumes’ D2% and D98% were insignificant for all sites except brain and nasopharynx. Dose maximum differences were within 2% for the majority of critical structures and target volumes. 3D gamma analysis results revealed that majority of plans satisfied the 2% and 2 mm criteria. CCT may be acquired for VMAT radiotherapy planning purposes instead of NCCT, since there is no clinically significant difference in dose calculations based on either image set.
Soil Bacteria as Potential Biological Control Agents of Fusarium Species Associated with Asparagus Decline Syndrome
Authors: De la Lastra Eduardo , Camacho María , Capote Nieves
Published: September 2021

To avoid the use of agrochemicals in agriculture, alternative methods are emerging to control plant pathogens. Some plant growth-promoting rhizobacteria (PGPR) can be used as biocontrol agents since they can induce protection against pathogens. The aim of this study was to evaluate the protective effect of several PGPR strains against the main Fusarium species involved in asparagus decline syndrome (ADS): F. proliferatum, F. oxysporum f. sp. asparagi and F. redolens. In vitro antagonism assays showed that all the bacteria inhibited the mycelium growth of the three Fusarium species. The most effective strains (Streptomyces fradiae Hvs6, Bacillus paralicheniformis Hvs2 and Bacillus velezensis FC37) were tested to evaluate their protective effect on asparagus plants inoculated with pathogenic Fusarium isolates. Strains FC37 and Hvs2 were the most effective in controlling pathogenic F. proliferatum and F. oxysporum f. sp. asparagi, but neither could protect against F. redolens isolates. The production of hydrolytic enzymes such as β-glucosidase, amylase and protease by these bacterial strains could be involved in the structural degradation of the fungal cell wall. In addition, the production of toxic volatile compounds, such as hydrogen cyanide, may inhibit the fungal growth, and the production of phosphate solubilizers could be related to the plant growth promotion. These results suggest that strains FC37 and Hvs2 could be used as potential biocontrol agents as a sustainable and environmentally friendly control strategy for ADS-affected fields.
Capturing Discriminative Information Using a Deep Architecture in Acoustic Scene Classification
Authors: Shim Hye-jin , Jung Jee-weon , Kim Ju-ho , Yu Ha-jin
Published: September 2021

Acoustic scene classification contains frequently misclassified pairs of classes that share many common acoustic properties. Specific details can provide vital clues for distinguishing such pairs of classes. However, these details are generally not noticeable and are hard to generalize for different data distributions. In this study, we investigate various methods for capturing discriminative information and simultaneously improve the generalization ability. We adopt a max feature map method that replaces conventional non-linear activation functions in deep neural networks; therefore, we apply an element-wise comparison between the different filters of a convolution layer’s output. Two data augmentation methods and two deep architecture modules are further explored to reduce overfitting and sustain the system’s discriminative power. Various experiments are conducted using the “detection and classification of acoustic scenes and events 2020 task1-a” dataset to validate the proposed methods. Our results show that the proposed system consistently outperforms the baseline, where the proposed system demonstrates an accuracy of 70.4% compared to the baseline at 65.1%.
Psoriasis—A Cancer Risk Factor?
Authors: Gheucă-Solovăstru Laura , Vâţă Dan , Halip Alina Ioana , Patraşcu Adriana , Cozma Andreea , Porumb-Andrese Elena
Published: September 2021

Psoriasis is not considered a strictly skin condition, but a complex disease with multisystem involvement due to the frequent associated comorbidities. We conducted a retrospective database study of 10,986 patients admitted in the interval January 2008–January 2019 to the Dermatology Clinic of the Iasi County “St. Spiridon” Emergency Hospital. Of the 10,986 patients admitted, 1288 were diagnosed with psoriasis. The association of malignancies was found in 40 of the psoriasis group cases and 399 of the control group cases that included various dermatological conditions. The calculation of Odds Ratios allowed us to determine if the patients with psoriasis could be at risk for certain malignancies. Thus, an association was suggested between psoriasis and central nervous system (CNS), upper aerodigestive tract cancer, endocrine cancer, bladder cancer, lung cancer, prostate cancer, breast cancer, or colorectal cancer. It is the first study of its kind in the northeastern region of Romania and can be the starting point for future long-term prospective cohort studies that will allow a more accurate data collection and a better understanding of the psoriasis–cancer relationship.