bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Exogenous pH on Cell Growth of Breast Cancer Cells
Authors: Lee Sungmun
Published: March 2021

Breast cancer is the most common cancer in women and the most life-threatening cancer in women worldwide. One key feature of cancer cells including breast cancer cells is a reversed pH gradient, and extracellular pH (pHe) of cancer cells is more acidic than normal cells. Cancer cells have lower pHe of ~ 6.7–7.1 and higher intracellular pH (pHi) of 7.4, while normal cells have pHe of 7.4 and lower pHi of 7.2. Here, we investigated how exogenous pH affected breast cancer cells. MDA-MB-231 cell lines were cultured in five different pHs, pH 6.0, pH 6.7, pH 7.4, pH 8.4, and pH 9.2 of medium. The cells were growing in pH 6.0 and pH 9.2 however, not as fast as in other pHs. Especially they were floating in more acidic conditions than pH 6.3. In alkaline pH (pH 8.4 and pH 9.2), more cells were early apoptotic and they were in S phase. In acidic pH (pH 6.0), more cells were late apoptotic or necrotic and more cells were at G2/M phase in acidic pH (pH 6.0 and pH 6.7). The results suggested that MDA-MB-231 cells experienced different cell growth and cell metabolism in different pHs.
An Intelligent Control Approach for Defect-Free Friction Stir Welding
Authors: Cobos Richard , Salas Santiago D. , Angulo Wilfredo , Liao T. Warren
Published: March 2021

An intelligent control approach is proposed as an alternative for the friction stir welding of an aluminum alloy. A validated empirical model is re-written from transfer functions to a set of ordinary differential equations, allowing to observe the force dynamics as a function of inputs of interest. A defect-free set-point is proposed for exploiting available labeled experimental data which defines operational boundaries and a region in which the probability of achieving defect-free welds with good mechanical properties is the highest. An intelligent controller in the fashion of a Recurrent Neural Network is constructed. Computational experiments were carried out to verify the adequacy in disturbance rejection as well as to visualize the capabilities in achieving the proposed defect-free set-point by the controller. The intelligent approach is compared with a set of decoupled proportional-integral controllers and a linear model predictive control strategy. From this study it is concluded that the intelligent controller shows superiority and good applicability for the studied problem.
New technique for false lumen coiling of spontaneous isolated superior mesenteric artery dissection
Authors: YAMAGUCHI HIDENORI , Murata Satoru , Ueda Tatsuo , Mine Takahiko , Onozawa Shiro , Hayashi Hiromitsu , Kumita Shin-ichiro
Published: March 2021

Background: Spontaneous isolated visceral artery dissection is rarely encountered. Endovascular intervention with good outcomes has become popular for patients with persistent symptoms or developing ischemia. We could perform life-saving treatment for a spontaneous isolated superior mesenteric artery dissection with a unique endovascular intervention.Case presentation: We describe the case of an 80-year-old man who presented with acute abdominal pain and a spontaneous isolated superior mesenteric artery dissection measuring 35 mm in major diameter and 6.6 mm in minor diameter on abdominal contrast-enhanced computed tomography. After admission, abdominal pain was progressive, and a repeated scan revealed progression of the dissection. As an endovascular intervention, via the bilateral femoral approach, detachable coils were placed in the false lumen of the superior mesenteric artery dissection through the false lumen under the micro-balloon occlusion at the point of re-entry and entry through the true lumen to prevent coil migration. Technical and clinical success was achieved without serious adverse events.Conclusion: Coil embolization using micro-balloon assistance combined with the double-catheter technique for a large entry and re-entry false lumen of a spontaneous isolated superior mesenteric artery dissection was useful and feasible.
Celastrol Attenuates the Remodeling of Pulmonary Vascular and Right Ventricular in Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats
Authors: li huayang , Liu Quan , Yue Yuan , Wang Shunjun , Huang Suiqing , Huang Lin , Li Luo , Zhang Yitao , Wu Zhongkai
Published: March 2021

PurposePulmonary arterial hypertension (PAH) is a progressive angio-proliferative disease with high morbidity and mortality. Although the histopathology is well described, its therapeutic effect is still unsatisfactory. This study investigated the treatment of celastrol on PAH rats right ventricular (RV) dysfunction, RV remodeling and pulmonary vascular remodeling and its possible mechanisms.MethodsPAH in rats was induced by monocrotaline (MCT) single subcutaneously injection. After daily delivery of Celastrol (1mg/kg) or vehicle via intraperitoneal injection for 4 weeks, the effects of celastrol on RV function, fibrosis, and pulmonary vascular remodeling were assessed. Macrophage infiltration, expression of inflammatory cytokines, including MCP-1, IL-1β, IL-6, IL-10 and the expression of NF-kB signaling pathway-associated protein, IkBα, p-IKKα/β and p65 were further detected. Finally, the effect of celastrol on human pulmonary artery smooth cells (HPASMCs) proliferation under hypoxia was studied in vitro.ResultsRats with PAH had degressive RV function, increased RV fibrosis and pulmonary arteries with interstitial thickening and prominent media hypertrophy. Treatment with celastrol improved RV function, attenuated RV fibrosis and pulmonary vascular remodeling. Significantly deceased macrophage infiltration, lower level of pro-inflammatory cytokines, higher level of anti-inflammatory cytokine and inhibited NF-κB signaling pathway were observed in lung tissues of rats with celastrol treatment. Moreover, celastrol significantly suppressed the proliferation of HPASMCs under hypoxia. Conclusions﻿We showed that, in rats with PAH, celastrol could improve RV function, attenuate RV and pulmonary vascular remodeling and the proliferation of HPASMCs under hypoxia. The suppression of NF-κB signaling pathway may be a part of the protective mechanism.
Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules
Authors: Ozono Yoshiyuki , Tamura Atsushi , Nakayama Shogo , Herawati Elisa , Hanada Yukiko , Ohata Kazuya , Takagishi Maki , Takahashi Masahide , Imai Takao , Ohta Yumi , Oshima Kazuo , Sato Takashi , Inohara Hidenori , Tsukita Sachiko
Published: March 2021

The V-shaped arrangement of hair bundles on cochlear hair cells is critical for the auditory sensing. However, regulation of hair bundle arrangements is not fully understood. Recently, defects in hair bundle arrangement were reported in postnatal Dishevelled-associating protein (Daple)-deficient mice. Here, we found that adult Daple-/- mice exhibited hearing disturbances over a broad frequency range through auditory brainstem response testing. Consistently, distorted patterns of hair bundles were detected in almost all regions, more typically in the basal region of the cochlear duct. In adult Daple-/- mice, apical microtubules were irregularly aggregated, and the number of microtubules attached to plasma membranes was decreased. Similar phenotypes were manifested upon nocodazole treatment in a wild type cochlea culture without affecting the microtubule structure of the kinocilium. These results indicate critical roles of Daple in hair bundle arrangement, through the orchestration of apical microtubule distribution, and thereby in hearing, especially at high frequencies.
Treatment of Persistent Post-traumatic Headache With the anti-CGRP Receptor Antibody Erenumab: a Case Report
Authors: Papenhoff Mike Christian , Dudda Marcel
Published: March 2021

Background: Calcitonin gene-related peptide (CGRP) has only recently emerged as a potential target in posttraumatic headache. To our knowledge this is the first description of a patient with persistent posttraumatic headache following severe traumatic head injury who has been treated with the CGRP-receptor-monoclonal antibody erenumab.Case presentation: A 56-year-old man presented with a 30-year history of frequent migraine-like posttraumatic headaches following a high-voltage head injury that he treated with an excessive intake of peripheral analgesics. Although amitriptyline was reasonably effective, it had to be discontinued when the patient developed restless legs syndrome in the second year of treatment. Under therapy with erenumab he achieved a rapid and stable reduction in his symptoms down to only 2–3 headache days per month without adverse events.Conclusions: This case demonstrates that the inhibition of the CGRP-receptor with erenumab could be an effective treatment option in migraine-like post-traumatic headache even decades after the traumatic event.
Virtual Voice Assistant Applications Improved Expressive Verbal Abilities and Social Interactions in Children with Autism Spectrum Disorder: A Single-Subject Experimental Study
Authors: Safi Mohammed F , Sadrani Badriya Al , Mustafa Ashraf
Published: March 2021

Background: Children with autism spectrum disorder (ASD) tend to have communication and social interaction deficits. Their impaired communication is derived from difficulties in acquiring language. The use of interactive technologies has been demonstrated to enhance verbal and non-verbal communication, as well as the social interaction tendencies of children with ASD. Artificial intelligence has played a growing role in the habilitation of children with ASD. However, little research exists on the possible roles and effectiveness of virtual voice assistants in developing language and social skills in children with ASD. This study examined the effects of using a voice assistant in children with ASD on two outcomes: speech skills (expressive verbal vocabulary and production of short phrases) and social interaction skills (playing/sharing). Methods: An interventional single-case design study was used to explore this concept using three children with ASD between the ages of 4 and 11 years. The participants used an accessible virtual voice assistant, Apple’s Siri, for three months. Pre- and post-intervention questionnaires and semi-structured interviews with mothers were administered to measure the communication and social interaction skills of the participating children. Results: Participant One, Two and Three showed a notable improvement in the total number of correct words produced with fewer attempts during the VVA intervention compared with the baseline phase. Further, all participants showed increases in the social interactions in the intervention phase, compared with the baseline phase. Finally, all the mothers noted improvement in their children’s speech intelligibility and social interactions. Conclusions: Results showed that the virtual voice assistant had positive effects on the speech and social interaction skills of children with ASD. The findings of this study implied that children with ASD can use readily available voice assistant software to improve their speech and social interaction skills. Furthermore, this study’s findings could be used to develop strategies to increase the availability of artificial intelligence infrastructure in schools and homes to help children with ASD.
Ganesh pilot study: The evolution of metabolism during different levels of NIV in healthy volunteers
Authors: Jonckheer Joop , Mekeirele Michael , Vanhonacker Domien , Costa Olivier R , Londersele Peter van , Diltoer Marc , Waele Elisabeth De
Published: March 2021

BackgroundMetabolism of critically ill adult patients is variable and influenced by intrinsic and extrinsic factors. Energy expenditure and thereby metabolism can be captured with indirect calorimetry (IC). An increased respiratory muscle use due to acute hypercapnic failure during Chronic Obstructive Pulmonary Disease exacerbation or other pulmonary problems may increase energy expenditure. Therefore, noninvasive ventilation (NIV) can be used in these conditions. It decreases the work of breathing by supporting muscle activity which will in turn cause a lower muscle metabolism. When this therapy fails, a higher mortality is observed due to delayed intubation. No good single early clinical parameter for NIV failure has been identified. Metabolism seems a promising parameter that has not been researched yet. We explored if IC connected to the NIV device will capture metabolic alterations due to different levels of support with NIV in healthy subjects. Patient and methodsIn this pilot study, metabolism objectified by Resting Energy Expenditure was measured with IC in 5 healthy subjects receiving NIV with increasing levels of support. Noninvasive hemodynamic monitoring was applied. Rate of perceived exertion was also assessed. ResultsDuring increasing levels of inspiratory support, linear regression showed increasing energy expenditure with a function of Y 1 =18,42*X+743,3 and decreasing levels of dyspnea with a function of Y 5 = -0,565*X + 4,42. Friedman test was not significantly different (p=0,364) for energy expenditure but was significantly different (p= 0,014) for the rate of perceived exertion. Further analysis of the rate of perceived exertion with Wilcoxon matched-pairs rank test showed no difference for the different levels of support compared to only positive end-expiratory pressure (PEEP).ConclusionIndirect calorimetry captures metabolic alterations induced by NIV treatment. Metabolism is influenced by increasing levels of support in healthy volunteers. Rate of perceived exertion is not correlated with metabolic alterations induced by NIV.
Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis
Authors: Tao Xiaolei , Chen Chunbo , Luo Weihong , Zhou Jing , Tian Jianwei , Yang Xiaobing , Hou Fanfan
Published: March 2021

Background
Sepsis is the most common trigger for AKI and up to 40% of mild or moderate septic AKI would progress to more severe AKI, which is associated with significantly increased risk for death and later CKD/ESRD. Early identifying high risk patients for AKI progression is a major challenge in patients with septic AKI.
Objectives
This study aims to evaluated the utility of combining renal cell arrest and damage biomarkers for predicting the progression of AKI in patient with sepsis.
Methods
This is a prospective, multicenter cohort study which enrolled adult patients with sepsis and initially developed stage 1 or 2 AKI from January 2014 to March 2018. AKI was diagnosed and staged according to 2012 KDIGO-AKI guidelines. Renal cell arrest biomarkers (urinary TIMP2*IGFBP7, u[TIMP-2]*[IGFBP7]) and renal damage biomarkers (urinary KIM-1[uKIM-1] and urinary IL-18 [uIL-18]) were measured at time of AKI clinical diagnosis, and the utility of biomarkers for predicting septic AKI progression alone or in combination were evaluated. The primary outcome was AKI progression defined as worsening of AKI stage. The second outcome was AKI progression with subsequent death during hospitalization.
Results
Among 433 screened patients, 149 patients with sepsis and stage 1 or 2 AKI were included, in which 63 patients developed progressive AKI and 49 patients subsequently died during hospitalization. u[TIMP-2]*[IGFBP7], uKIM-1 and uIL-18 independently predicted the progression of septic AKI in which u[TIMP-2]*[IGFBP7] showed the greatest AUC (0.745; 95%CI, 0.667–0.823) as compared to uKIM-1 (AUC 0.719; 95%CI 0.638-0.800) and uIL-18 (AUC 0.619; 95%CI 0.525–0.731). Combination of u[TIMP-2]*[IGFBP7] with uKIM-1 improved the performance of predicting septic AKI progression with AUC of 0.752. u[TIMP-2]*[IGFBP7], alone or combined with uKIM-1/uIL-18, improved the risk reclassification over the clinical risk factor model alone both for the primary and secondary outcomes, as evidenced by significant category-free net reclassification index.
Conclusions
Combination of renal cell arrest and damage biomarkers enhanced the prediction of AKI progression in patients with sepsis and improved risk reclassification over the clinical risk factors.
ANALYSIS OF THE INCIDENCE RATE OF COLORECTAL CANCER AMONG RESIDENTS OF THE SUMY REGION
Authors: Kostiuchenko V.V. , Moskalenko Y.V. , Tarasenko S.V. , Vynnychenko O.I. , Shevchenko V.V. , Vynnychenko I.O.
Published: March 2021

Before the middle of the 20th century, colorectal cancer (CRC) was a fairly rare disease. A significant increase in the prevalence of this pathology began after this period and was accompanied by a doubling of indicators in each subsequent decade. By 2030, the incidence rate of colorectal cancer is expected to increase by 60% or more than 2.2 million new cases and 1.1 million annual deaths. This situation is due to the development of countries with transitional economies, to which Ukraine also belongs.
Chemical Characteristics and Antioxidant Activity of Astaxanthin Extracted from Shrimp Residues Using Soybean Oil
Authors: e Silva Denise , Damasceno Karla , Ribeiro Penha , Alcântra Maristela , Cordeiro Angela , Passos Thaís , de Sousa Jr. Francisco , de Assis Cristiane
Published: March 2021

Shrimp residues generated by the seafood industrial processing are generally discarded in the environment without any further treatment, leading to a loss of nutritional value such as proteins, lipids, chitin, carotenoids, and minerals. This practice can lead to environmental problems due to the decomposition of these substances in the air. The objective of this study was to extract astaxanthin-rich materials from shrimp (Litopenaeus vannamei) industrial residue (WO) and smaller-scale residue meal (MO) in processing shrimp with soybean oil to evaluate their physicochemical characteristics, fatty acid content, and antioxidant potential. WO and MO were found to contain 27.48 and 33.34 µg g-1 of astaxanthin, respectively. The physicochemical properties of pigmented oils are established by legislation. The MO material showed significantly higher antioxidant activity compared to the soybean oil (control), especially when based on the oxygen radical absorbance capacity (ORAC) test, which showed antioxidant activity of 0.484 and 0.264 µmol eq Trolox g-1 for the MO and soybean oil (control), respectively, possibly accompanying their respective astaxanthin contents in the MO sample. The pigmented oils from both the shrimp residues showed significant potential for being used in the food industry due to their affordability and their high antioxidant activity.
Profitability improvement of a reservoir power station based on virtual reservoir bidding
Authors: Zhu Yanmei , Huang Weibin , Chen Shijun , Ma Guangwen , Liu Yue
Published: March 2021

Compared with a run-of-river power station, a reservoir power station (RPS), with the capacity of a seasonal regulation function and more, has the unique and irreplaceable attributes of its role in the power grid and society at large. However, under the current power system and market environment in China, these attributes cannot be effectively utilised, resulting in heavy losses to enterprises. It is inevitable for RPSs to participate in the market; therefore, how to improve the profitability of RPS enterprises has become an urgent problem requiring a solution. Based on the control effect on run-off of RPS in a river basin, a new cascade hydropower bidding method and the associated bidding process are proposed. The market entities of the new bidding method are composed of cascade hydropower joints with the RPS as the boundary. The bidding unit benefit-sharing mechanism is constructed, and the accounting method for the benefit-sharing price of the RPS is established, which finally achieves the goal of improving the profitability of the RPS enterprise.
Recent Advances in Diagnostic and Therapeutic Approaches for Breast Cancer: A Comprehensive Review
Authors: Noor Fatima , Noor Ayesha , Ishaq Ali Raza , Farzeen Iqra , Saleem Muhammad Hamzah , Ghaffar Kanwal , Aslam Muhammad Farhan , Aslam Sidra , Chen Jen-Tsung
Published: March 2021

A silent monster, breast cancer, is a challenging medical task for researchers. Breast cancer is a leading
cause of death in women with respect to other cancers. A case of breast cancer is diagnosed among women
every 19 seconds, and every 74 seconds, a woman dies of breast cancer somewhere in the world. Several risk
factors, such as genetic and environmental factors, favor breast cancer development. This review tends to provide
deep insights regarding the genetics of breast cancer along with multiple diagnostic and therapeutic approaches
as problem-solving negotiators to prevent the progression of breast cancer. This assembled data mainly
aims to discuss omics-based approaches to provide enthralling diagnostic biomarkers and emerging novel
therapies to combat breast cancer. This review article intends to pave a new path for the discovery of effective
treatment options.
Anti-breast Cancer Potential of Natural and Synthetic Coumarin Derivatives
Authors: Song Feng , Huo Xiankai , Guo Zeying
Published: March 2021

:

Breast cancer is the most frequent female cancer and one of the leading causes of cancer
death in women. There are many chemotherapy agents available for the treatment of breast cancer.
Still, the current therapeutic options have not fulfilled the desired outcomes, especially for drug-resistant
breast cancer therapy. Thus, there is an urgent need to develop novel anti-breast cancer
agents. Coumarin is ubiquitous in natural and synthetic bioactive compounds, and coumarin derivatives
readily interact with a variety of enzymes and receptors in breast cancer cells. Moreover, the
coumarin-based irosustat as the first-generation steroid sulfatase inhibitor in breast cancer is under
clinical evaluation, revealing the potential of coumarin derivatives as novel anti-breast cancer
agents. This review aims to describe the recent development of natural and synthetic coumarin derivatives
with anti-breast cancer potential, covering the articles published from 2015 to 2020.
Evaluation of Differentiation Quality of Several Differentiation Inducers of Bone Marrow-derived Mesenchymal Stem Cells to Nerve Cells by Assessing Expression of Beta-tubulin 3 Marker: A Systematic Review
Authors: Fath Mohsen Karami , Zahedi Farhad , Hashemi Zahra Sadat , Khalili Saeed
Published: March 2021

Neurological diseases have different etiological causes. Contemporary, developing an effective
treatment for these diseases is an ongoing challenge. Cell therapy is recognized as one of
the promising solutions for the treatment of these diseases. Amongst various types of stem cells,
bone marrow-derived mesenchymal stem cells (BM-MSC) are known to be the most widely used
stem cells. These cells are endowed with appealing properties such as the ability to differentiate into
other cell types, including the muscle, liver, glial, and nerve cells. In this review study, we have
systematically evaluated the ability of a variety of chemical compounds used in the last ten years to
differentiate BM-MSCs into neurons by examining the expression level of beta-tubulin 3 protein.
The present study is a systematic search performed at three separate databases, including PubMed,
ScienceDirect, and Embase from August 2009 to August 2019. The search results in the three mentioned
databases were 323 articles and finally, 8 articles were selected and carefully examined considering
the inclusion and exclusion criteria. The results showed that different chemical compounds
such as ROCK inhibitors, sex steroid hormones, bFGF, NGF, Noggin, 4 OHT, TSA, VPA,
Antidepressants, Neurosteroids (Dex and E2), and DHA are involved in different signaling pathways,
such as ERK, AKT, BMP, DHA / GPR40, Rho-dependent phosphorylation, and histone
deacetylase inhibitors. Further investigation of these signaling pathways may open the way for better
differentiation of BM-MSCs into neurons.
Selective Cytotoxic Effects of 5-Trifluoromethoxy-1H-indole-2,3-dione 3-Thiosemicarbazone
Derivatives on Lymphoid-originated Cells
Authors: Danışman-Kalındemirtaş Ferdane , Erdem-Kuruca Serap , Akgün-Dar Kadriye , Karakaş Zeynep , Soylu Özge , Karali Nilgün
Published: March 2021

Aim:

The present study aims to identify the anticancer effect of novel 1H-indole-2,3-dione 3-
thiosemicarbazone derivatives. These compounds could be promising anticancer agents in leukemia treatment.

Background :

Conventional chemotherapeutic agents accumulate in both normal and tumor cells due to nonspecificity.
For effective cancer treatment, new drugs need to be developed to make chemotherapeutics selective
for cancer cells. The ultimate goal of cancer treatment is to reduce systemic toxicity and improve the quality of
life.

Method:

In this study, the anticancer effects of 5-trifluoromethoxy-1H-indole-2,3-dione 3-thiosemicarbazone
derivatives (A-L) were investigated in chronic myelogenous leukemia K562, Burkitt’s lymphoma P3HR1, acute
promyelocytic leukemia HL60 cells, and vincristine-resistant sublines of K562 and P3HR1 cells. Additionally,
the compounds were tested on lymphoid-derived cells from ALL patients. In order to investigate the particular
mechanism of death caused by the cytotoxic effects of the compounds, immunohistochemical caspase 3 staining
was performed in P3HR1 cells, and the resulting apoptotic activities were demonstrated.

Result:

All tested compounds have been found to have cytotoxic effects against lymphoma cells at
submicromolar concentrations (IC50= 0.89-1.80 μM). Most compounds show significant selectivity for the
P3HR1 and P3HR1 Vin resistance. The most effective and selective compound is 4-bromophenyl substituted
compound I (IC50=0.96 and 0.89 μM). Cyclohexyl and benzyl substituted compounds D and E have also been
found to have cytotoxic effects against K562 cell lines (IC50=2.38 μM), while the allyl substituted compound C is
effective on all cell lines (IC50=1.13-2.21 μM). 4-Fluorophenyl substituted F compound has been observed to be
effective on all cells (IC50=1.00-2.41 μM) except K562 cell. Compound C is the only compound that shows
inhibition of HL-60 cells (IC50= 1.13 μM). Additionally, all compounds exhibited cytotoxic effects on lymphoidderived
cells at 1μM concentration. These results are in accordance with the results obtained in lymphoma cells.

Conclusion:

All compounds tested have submicromolar concentrations of cytotoxic effects on cells. These
compounds hold potential for use in future treatments of leukemia.
Media Fill Test for Validation of Non-Radioactive Kit for Radiopharmaceutical
Preparations
Authors: Mariani Marco , Vincenti Sara , Scotognella Teresa , Bruno Stefania , Boninti Federica , Cambieri Andrea , Damiani Gianfranco , Laurenti Patrizia , Giordano Alessandro
Published: March 2021

Introduction:

The administration of improperly prepared intravenous fluids might determine
healthcare-associated infections. Quality and sterility assurance in radiopharmaceutical manufacturing
products must be evaluated by media fill tests that simulate the performance of the aseptic
manufacturing procedure. The aim of this study is to show a methodological modification of a
specific step of media fill tests, giving an overview of economic and organizational advantages that
it might bring.

Materials And Methods:

Media fill tests were conducted to ensure that they reproduce as strictly
as possible the routine aseptic production process with all the critical steps described in the internal
Standard Operating Procedures. We introduced an innovative modification in step 1: instead of using
a completely decayed 99Mo/99mTc generator, we used a99Mo/99mTc generator just before its disposal,
eluting it with saline solution aspirated in an empty vacuum vial.

Results:

A total of 47 production runs were performed, and a total of 799 vials were tested for
sterility. Thirteen nuclear medicine technicians were assessed. We found contamination in 1% of
the total number of vials analyzed.

Conclusions:

The modification proposed shows several advantages: reduction of the costs of the
media fill tests, the possibility to manage these without referring to external services, and decreased
injuries for technicians who perform the tests.
Genetičko testiranje recesivnih monogenskih bolesti
Authors: Dejhalla Ema , Dević Pavlić Sanja , Peterlin Borut , Ostojić Saša , Pereza Nina
Published: March 2021

Pravovremeno postavljanje ispravne dijagnoze recesivne monogenske bolesti u oboljele osobe od iznimne je važnosti za mogućnosti provođenja dostupnih metoda prevencije razvoja bolesti ili specifične odnosno simptomatske terapije kada je bolest već razvijena. Nadalje, važna karika u prevenciji i praćenju vjerojatnosti pojavljivanja recesivnih monogenskih bolesti su različite mogućnosti genetičkog probira kojima je moguće otkriti nositelje genetske predispozicije za bolest, koji su najčešće zdrave osobe, ali mogu prenijeti gensku promjenu na potomstvo. U ovom preglednom članku prikazane su najnovije spoznaje vezane uz definiciju, učestalost i dijagnostiku recesivnih monogenskih bolesti, uključujući dijagnostičko genetičko testiranje, utvrđivanje statusa nositelja, probir u populaciji i prošireni genomski probir nositelja.
Bouveretov sindrom kao rijetka komplikacija kolecistolitijaze
Authors: Klarica Lucia , Grbas Harry
Published: March 2021

Cilj: Prikazati slučaj pacijentice s akutnim abdominalnim bolom i Bouveretovim sindromom kao rijetkom komplikacijom kolecistolitijaze. Prikaz slučaja: Osamdesetogodišnja pacijentica primljena je u hitnu medicinsku službu zbog bolova u epigastriju i gornjem abdomenu uz povraćanje tamnog sadržaja koji traju unazad tri dana. Posljednja stolica pacijentice bila je uredna, uredne diureze i pacijentica je bila afebrilna. U laboratorijskim nalazima vrijednosti upalnih parametara bile su neznatno povišene. Učinjen je ultrazvuk abdomena koji je pokazao skvrčeni žučnjak s kamencima. Nativna snimka abdomena nije pokazala znakove pneumoperitoneuma i ileusa. Pacijentici je ordinirana ulkusna terapija s naglaskom da se javi na kontrolni pregled ako se stanje pogorša. Dva dana nakon prijama pacijentici se stanje pogoršalo te dolazi u gastroenterološku ambulantu, gdje joj je učinjena ezofagogastroduodenoskopija (EGDS) koja je pokazala tamnozeleni sadržaj u lumenu jednjaka i želuca te zaglavljen velik kamenac u pilorusu, zbog čega je pacijentica hospitalizirana. Drugog dana hospitalizacije pacijentici se ponovio EGDS s neuspješnim ishodom. Trećeg dana hospitalizacije pacijentici je neuspješno učinjena hitna eksplorativna laparoskopija i operativni zahvat se konvertirao u desnu subkostalnu laparotomiju, te je gastrotomijom ekstrahiran žučni kamenac. Pacijentici je dalje učinjena gastroenteroanastomoza i enteroenteralna anastomoza po metodi Braun, uveden je dren subhepatalno i rana je zašivena po slojevima. Postoperativni tijek kompliciran je infekcijom operacijske rane. Pacijentica je otpuštena dvadesetdrugog postoperativnog dana te nije imala bilijarnih smetnji u sljedećih šest mjeseci praćenja. Zaključak: Uzrok akutnog abdominalnog bola nerijetko može biti i komplikacija kolecistolitijaze. U rijetkim slučajevima kolecistolitijaza se može komplicirati žučnim kamencem prisutnim izvan žučnjaka koji migirira kroz biliogastričnu ili bilioduodenalnu fistulu s opstrukcijom pilorusa (Bouveretov sindrom), što predstavlja životno ugrožavajuće stanje uz smrtnost od 12 % do 30 %.
Telehealth Transition Assistance Program for Acute Spinal Cord Injury Caregivers: Protocol for a Mixed-Methods, Randomized Controlled Trial
Authors: Perrin Paul B , McDonald Scott D , Watson Jack D , Pierce Bradford S , Elliott Timothy R
Published: March 2021

Background

While spinal cord injury (SCI) caregiving can be a rewarding experience, caregivers often experience reduced mental and physical health.

Objective

This article describes the methodology of a study examining the efficacy of a newly developed telehealth Transition Assistance Program (TAP) for caregivers of individuals with acute SCI.

Methods

A mixed-methods, randomized controlled trial is comparing TAP outcomes to that of a standard-of-care control. The study is recruiting for 48 months and incorporating quantitative outcome measures.

Results

This study was funded by the Craig H. Neilsen Foundation in April 2017. It was approved by the institutional review boards at Virginia Commonwealth University and the Hunter Holmes McGuire Veterans Affairs Medical Center that same year. Participant recruitment and data collection began in 2018.

Conclusions

This study is implementing and testing an SCI caregiver intervention unlike any created before, targeting a critical time period that, until now, other SCI caregiver interventions have overlooked. Research personnel intend to disseminate the intervention and study findings through the publication of manuscripts and presentations at conferences. If the current study shows improvements in caregiver or patient well-being, the TAP for SCI caregivers could become part of the standard of care for acute SCI.

Trial Registration

ClinicalTrials.gov NCT03244098; https://www.clinicaltrials.gov/ct2/show/NCT03244098

International Registered Report Identifier (IRRID)

DERR1-10.2196/28256
An Enhanced Recurrent Convolutional Neural Network for Predicting the Status Stage of Patients with Chronic obstructive Pulmonary Diseases: Method Design (Preprint)
Authors: Li Wei , Hao Tianyong , Mai Zhanjie , Yu Pengjiu , Liu Chunli , Luo Qun
Published: March 2021

BACKGROUND

Chronic Obstructive Pulmonary Disease (COPD) is the third leading cause of death in China and has caused serious affect to health and life quality. However, the status stage of a patient is difficult to be accurately assessed because of dynamic changes in the condition and complex risk factors. A rapid and accurate methods to predict disease stage of COPD patients is of great significance.

OBJECTIVE

This study aims to explore an enhanced recurrent convolutional neural networks model for predicting correct staging of patients with COPD in China for assistant disease prevention and treatment.

METHODS

Data was collected from The First Affiliate Hospital of Guangzhou Medical University, which had standardized disease registration and follow-up management for 5108 patients with COPD. Our enhanced recurrent convolutional neural network consists of a bidirectional LSTM layer, a convolutional layer, a max-pooling layer, and an output layer.

RESULTS

The model proposed was evaluated on the real-world clinical dataset of 5108 COPD patients to predict the state stage of the disease. The performance of the proposed model achieved 93.2% in terms of accuracy, outperforming a list of baseline models.

CONCLUSIONS

This paper proposes an enhanced recurrent convolutional neural network model which is experimented on a real-world clinical dataset containing around 5,000 patients with COPD. The proposed model achieves the best performance on all evaluation metrics indicating its feasibility in predicting the state stage of diseases.
Automated Size Recognition in Pediatric Emergencies Using Machine Learning and Augmented Reality: Within-Group Comparative Study (Preprint)
Authors: Schmucker Michael , Haag Martin
Published: March 2021

BACKGROUND

Pediatric emergencies involving children are rare events, and the experience of emergency physicians and the results of such emergencies are accordingly poor. Anatomical peculiarities and individual adjustments make treatment during pediatric emergency susceptible to error. Critical mistakes especially occur in the calculation of weight-based drug doses. Accordingly, the need for a ubiquitous assistance service that can, for example, automate dose calculation is high. However, few approaches exist due to the complexity of the problem.

OBJECTIVE

Technically, an assistance service is possible, among other approaches, with an app that uses a depth camera that is integrated in smartphones or head-mounted displays to provide a 3D understanding of the environment. The goal of this study was to automate this technology as much as possible to develop and statistically evaluate an assistance service that does not have significantly worse measurement performance than an emergency ruler (the state of the art).

METHODS

An assistance service was developed that uses machine learning to recognize patients and then automatically determines their size. Based on the size, the weight is automatically derived, and the dosages are calculated and presented to the physician. To evaluate the app, a small within-group design study was conducted with 17 children, who were each measured with the app installed on a smartphone with a built-in depth camera and a state-of-the-art emergency ruler.

RESULTS

According to the statistical results (one-sample t test; P=.42; α=.05), there is no significant difference between the measurement performance of the app and an emergency ruler under the test conditions (indoor, daylight). The newly developed measurement method is thus not technically inferior to the established one in terms of accuracy.

CONCLUSIONS

An assistance service with an integrated augmented reality emergency ruler is technically possible, although some groundwork is still needed. The results of this study clear the way for further research, for example, usability testing.
Therapeutic potential of mesenchymal stem cell-derived extracellular vesicles in regenerative endodontics
Authors:   Schönleinstrasse 2, 8032 Zurich, Switzerland, Ivica A , Zehnder M , Weber FE
Published: March 2021

Regenerative endodontic procedures are an alternative to conventional root-canal treatment and apexification. There are two different tissue engineering approaches that are currently followed, both aiming at the colonisation of the cleaned pulp space by pluripotent cells and subsequent pulp regeneration. Firstly, the transplantation of mesenchymal stem cells (MSCs), and secondly a cell-free strategy that relies on bioactive molecules to trigger the recruitment of the patient’s own cells. The first approach is hampered by costs and regulatory issues. Despite great initial enthusiasm with a clinically used cell-free approach that relies on induced bleeding into the pulp space, results have been revealed to be rather unpredictable, and mere repair rather than regeneration of the pulp-dentin complex is what is typically achieved. Moreover, the extent of further root development is variable, and the concept is limited to immature teeth. This article discusses a third possible way of regenerative endodontics that involves the application of MSC-derived exosomes. These are extracellular vesicles that contain proteins, lipids, and nucleic acids, reflecting the secretome of MSCs. Based on the first in vitro and in vivo studies, exosomes appear to be a potent tool to improve pulp regeneration. This narrative review aims to investigate the therapeutic use of human MSCs or dental pulp-derived exosomes in regenerative endodontics. Furthermore, the focus of this review is on targeting important questions that should be investigated in future in-vivo and clinical studies, such as the choice of scaffold material for exosome delivery into the pulp space.
K-Means Algorithm to Group Students’ Academic Status at STMIK Palangka Raya
Authors: Rusdiana Lili
Published: March 2021

As one of clustering methods K-Means algorithm can be used to group 93 data of STMIK Palangka Raya students into 3 namely pass, active, and inactive. The stages of it produce 9 iterations since the displacement of the group in which there is a movement from one group to another because the change value of the object function. The iteration can be stopped at the 9th because the value of the object function change is below the given threshold value of 0.1 and the absence of group movement in the data used. The 9 iterations that occur indicate the rise and fall the change value of object function and data transfer in the location of the group.
PEMANFAATAN SISTEM IJC (iLearning Journal Center) SEBAGAI MEDIA E-JOURNAL PADA PERGURUAN TINGGI DAN ASOSIASI
Authors: Oganda Fitra Putri
Published: March 2021

Pada masa di era kecanggihan teknologi saat ini, sehingga informasi tidak lagi dalam bentuk konvensional alias tidak terkomputerisasi namun sekarang sudah diterapkan elektronik yang disajikan sehingga dapat memudahkan pengguna. Ada beberapa perguruan tinggi yang dimana dalam pengelolaan serta publikasi karya ilmiah tersebut masih menggunakan sistem bentuk konvensional sehingga memiliki permasalahan-permasalahan seperti penerbitan jurnal yang rumit, terdapat data yang terpisah dikarenakan membutuhkan waktu yang cukup lama serta biaya cukup besar. Artikel ataupun karya ilmiah pada beberapa perguruan tinggi yang belum memenuhi syarat ketentuan dikti perihal Akreditasi Jurnal yang mengharuskan karya ilmiah atau jurnal tersebut harus dikelola secara online dalam pengelolaan yang dilakukan lebih efisien dan efektif. Setelah peneliti menganalisa menggunakan suatu metode analisis meliputi pemanfaatan jurnal, koleksi jurnal sesuai dengan kebutuhan pengguna, metode penelitian menggunakan metode deskriptif dan metode studi pustaka. Sehingga dapat dengan mudah untuk mengetahui bahwa sistem iJC (iLearning Journal Center) ini memiliki begitu banyak manfaat bagi beberapa perguruan tinggi dan Asosiasi.
Modifikasi Kunci Simetris Caesar Cipher dan OTP Menggunakan Algoritma Genetika Pada Steganografi
Authors: Ariyus Dony , Kurniasih Julia , Profesi Dwinda Etika
Published: March 2021

Masalah keamanan data merupakan hal yang penting, karena data harus dijaga keamanan dan keutuhannya mulai dari proses pengiriman sampai diterima oleh pihak yang seharusnya. Kriptografi dan steganografi merupakan alternatif cara menjaga keamanan data. Informasi dapat diacak dan disembunyikan melalui media lain seperti media suara atau gambar, sehingga pesan yang ingin disampaikan terjaga kerahasiaannya. Penyandian/pengacakan informasi dapat dilakukan dengan menggunakan algoritma Caesar Cipher dan One Time Pad yang merupakan algoritma kriptografi kunci simetris. Kunci sebagai bagian penting dari algoritma kriptografi perlu dikembangkan/dimodifikasi untuk mendapatkan kekuatan keamanan informasi/teks yang akan disampaikan. Pada penelitian ini dilakukan modifikasi kunci algoritma kriptografi Caesar Cipher dan One Time Pad dengan menggunakan algoritma genetika yang merupakan salah satu jenis algoritma optimasi. Cipherteks (teks teracak) hasil algoritma kriptografi disisipkan pada media gambar untuk menghasilkan gambar sandi. Pengujian kekuatan cipherteks dilakukan dengan melihat frekuensi pengulangan karakternya. Dari hasil pengujian diketahui bahwa modifikasi kunci simetris dengan menggunakan algoritma genetika memberikan kekuatan keamanan yang cukup baik terhadap cipherteks, dilihat dari persentase pengulangan karakternya yang rendah.
Implementasi Metode Advance Encryption Standard dan Least Significan Bit pada Kriptografi Citra Digital
Authors: Aisa Sitti , Aini Nurul
Published: March 2021

Perkembangan digital kian pesat tanpa kita sadari, salah satunya adalah kehadiran smartphone sudah menjadi salah satu kebutuhan pokok untuk masyarakat. Dimana, smarphone tidak hanya digunakan sebagai alat komunikasi tetapi juga sebagai alat bertukar informasi. Sistem pertukaran data dan informasi melalui Smartphone Android tidak memberi jaminan akan keamanan informasi khususnya untuk data yang bersifat rahasia. Pertukaran data dan informasi  dengan cara seperti ini rentang terhadap bahaya pencurian data, sehingga perlu mencari solusi untuk hal tersebut. Diperlukan suatu  sistem pengamanan data dan informasi terutama untuk data yang bersifat pribadi dan rahasia sehingga dapat sampai ke tangan yang berhak menerima. Salah satu metode yang sering digunakan untuk mengatasi hal tersebut dengan teknik kriptografi.salah satu metode yang digunakan adalah AES dan LSB Dengan metode AES ini, data diubah kedalam bentuk teks yang tidak diketahui maknanya agar orang lain tidak bisa membacanya dan untuk menghindari kecurigaan kepada orang lain maka si A menggunakan teknik steganografi dengan metode LSB. Aplikasi ini untuk mengamankan data yang akan dikirmkan pengamanan dilakukan dengan dua tahapan yang pertama tahap penyamaran pesan dengna metode AES dan tahap kedua adalah tahap penyisipan pesan dengan metode LSB, dimana  cipherteks yang disimpan dalam bentuk file text, serta dapat disisipkan ke dalam file citra.
DETEKSI EMOSI MEDIA SOSIAL MENGGUNAKAN TERM FREQUENCY- INVERSE DOCUMENT FREQUENCY
Authors: Rohman Arif Nur , Handayani Riska Dwi , Y. P. Ryan Dwi , Kusrini Kusrini
Published: March 2021

Pada saat ini, manusia cenderung mengekspresikan pendapat, dan emosi melalui media sosial. Keterbukaan ekspresi pada media sosial membuat batasan batasan pribadi seseorang menjadi lebur. Orang tidak lagi sungkan menulis kehidupan pribadinya melalui postingan status pembaharuan untuk dilihat oleh orang lain. Penulis mencoba menggunakan data dari media sosial agar dapat dilakukan analisis untuk mendapatan informasi kepribadian termasuk emosi. Sebelum dianalisis, data dilakukan pra pemrosesan membuang symbol dan icon, normalisasi teks, stemming dan membuang stopword terlebih dahulu untuk memperbaiki data dari media sosial yang menggunakan bahasa tidak baku. Pra pemrosesan memperoleh hasil akhir 365 dataset yang dipisah menjadi 265 data latih dan 100 data uji. Tiap data sudah memiliki label emosi yang diberikan secara manual oleh pengguna facebook selaku penulis dari postingan. Perhitungan dilakukan dengan TF IDF untuk mendapatkan bobot tiap kata atau token, lalu label emosi data latih dengan nilai tertinggi diberikan pada data yang diuji. Dari 100 data uji diperoleh akurasi 59%, selebihnya 41% tidak akurat. Emosi dianggap hal penting yang berpengaruh pada seseorang dalam pengambilan keputusan, sehingga deteksi emosi seseorang dapat dimanfaatkan sebagai input sistem pendukung keputusan penerimaan pegawai dan konseling kepribadian.
OTOMATISASI STOCK OPNAME PADA SENAYAN LIBRARY MANAGEMENT SYSTEM
Authors: Sastrawangsa Gde , Sumiari Ni Kadek , Karuna I Gede Bagus Vedanta Maha
Published: March 2021

Stock opname adalah kegiatan pemeriksaan fisik terhadap semua buku yang terdata dalam suatu perpustakaan dengan cara membandingkan seluruh koleksi literatur yang ada pada perpustakaan saat ini dengan jumlah koleksi literatur yang tercatat pada database. Pendataan ini berguna untuk mengetahui jumlah buku yang ada dan jumlah buku yang hilang atau masih terpinjam. Senayan Library Management System (SliMS) adalah Open Source Software (OSS) berbasis web untuk memenuhi kebutuhan automasi perpustakaan sehingga dapat memudahkan perpustakaan dalam mengelola koleksi literatur yang ada. Salah satu fitur yang ada pada SLiMS adalah dapat membantu melakukan stock opname atau inventarisasi. Proses stock opname pada SLiMS masih terbilang cukup lambat, karena prosesnya dilakukan dengan cara mengetikkan satu persatu kode buku ke dalam sistem. Salah satu solusi untuk mengatasi permasalahan tersebut adalah dengan membuat aplikasi barcode scanner pada mobile device berbasis sistem operasi Android. Aplikasi ini dibangun dengan menggunakan Android Studio, serta dibangun web service sebagai backend untuk menghubungkan data antara aplikasi Android dengan SLiMS. Penelitian ini menghasilkan aplikasi barcode scanner berbasis Android yang dapat mempermudah dan mempercepat proses stock opname pada SLiMS.