bluuebunny/crossref_metadata_2025 huggingface dataset:
L-Cysteine Upregulates Testosterone Biosynthesis and Blood–Testis Barrier Genes in Cultured Human Leydig Cells and THP-1 Monocytes and Increases Testosterone Secretion in Human Leydig Cells
Authors: Justin Margret Jeffrey , Jain Sushil K.
Published: September 2024

Leydig cells are the primary source of testosterone or androgen production in male mammals. The blood–testis barrier (BTB) maintains structural integrity and safeguards germ cells from harmful substances by blocking their entry into the seminiferous tubules. L-cysteine is essential to the production of glutathione, a powerful antioxidant crucial to protecting against oxidative stress-induced damage. Animal studies have demonstrated the protective effect of L-cysteine in preventing testicular damage caused by chemicals or radiation. This study examines whether L-cysteine enhances the expression of testosterone biosynthesis and the BTB genes in human Leydig cells and THP-1 monocytes. The Leydig cells and THP-1 monocytes were treated with L-cysteine for 24 h. RNA was extracted following treatment, and the gene expression was analyzed using quantitative RT-PCR. Testosterone levels in the cell supernatant were measured using an ELISA kit. L-cysteine treatment in Leydig cells significantly upregulated the expression of CYP11A1 (p = 0.03) and the BTB genes CLDN1 (p = 0.03), CLDN11 (p = 0.02), and TJP1 (p = 0.02). Similarly, L-cysteine significantly upregulated the expression of CYP11A1 (p = 0.03) and CYP19A1 (p < 0.01), and the BTB genes CLDN1 (p = 0.04), CLDN2 (p < 0.01), CLDN4 (p < 0.01), CLDN11 (p < 0.01), and TJP1 (p = 0.03) in THP-1 monocytes. Further, L-cysteine supplementation increased the testosterone secretion levels in human Leydig cells. The findings suggest that L-cysteine supplementation could be used as an adjuvant therapy to promote the integrity of the BTB genes, testosterone biosynthesis and secretion, and the maintenance of testicular functions, which in turn mitigates the risk of male infertility.
Unsuppressed HIV Viral Load and Related Factors in Patients Receiving Antiretroviral Treatment in Tanganyika Province, Democratic Republic of Congo (DRC)
Authors: Luhembwe Michel , Ingwe Richard , Lulebo Aimée , Nkamba Dalau , Ditekemena John
Published: September 2024

Antiretroviral treatment (ART) has revolutionized the management of the human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS), enabling long-term viral load (VL) suppression in patients. Despite the proven effectiveness of ART, a significant proportion of patients with HIV receiving ART fail to achieve viral load suppression (VLS). This study aimed to identify factors associated with low VLS in the Tanganyika province. An unmatched case–control study was conducted from January 2022 to June 2023, including 22 care facilities with viral load data. Data were collected from patient records. For each reviewed record, the patient was invited for an interview upon providing informed consent. Data were analyzed using SPSS version 27. In a multivariable binary logistic regression model, variables with a p-value < 0.05 and a 95% confidence interval for the adjusted odds ratio were considered significantly associated with unsuppressed VL. A total of 462 individuals, including 156 cases and 306 controls, were included in the study. The mean age (standard deviation) of participants was 42.12 (±11.6) years. The following covariates were significantly associated with unsuppressed VL: poor HIV status disclosure to a confidant [adjusted OR = 2.10, 95% CI (1.33–3.31), p = 0.001], poor ART adherence [adjusted OR = 2.01, 95% CI (1.25–3.23), p = 0.004], ART interruption [adjusted OR = 3.43, 95% CI (2.00–5.88), p < 0.001], no participation in support groups [adjusted OR = 2.16, 95% CI (1.25–3.71), p = 0.005], baseline WHO clinical stage 3 and 4 [adjusted OR = 2.24, 95% CI (1.32–3.79), p = 0.003], opportunistic infections (OIs) [adjusted OR = 2.30, 95% CI (1.27–4.16), p = 0.006], and non-communicable chronic diseases (NCDs) [adjusted OR = 2.30, 95% CI (1.10–4.79), p = 0.026]. Given the clear association between several factors and unsuppressed VL, prevention should involve the implementation of innovative strategies targeting at-risk patient groups. Strengthening the monitoring of these factors among active patients at each appointment is recommended to achieve this goal.
Effectiveness of Transcranial Direct Current Stimulation (tDCS) during a Virtual Reality Task in Women with Fibromyalgia—A Randomized Clinical Study
Authors: da Silva Thaís Nogueira , Ribeiro Vivian Finotti , Apaza Margot Carol Condori , Romana Lívia Gallerani , de Moraes Íbis Ariana Peña , Dias Eduardo Dati , Roizenblatt Suely Steinschreiber , Martinez Juliana Perez , Magalhães Fernando Henrique , Massa Marcelo , Ré Alessandro Hervaldo Nicolai , de Araújo Luciano Vieira , da Silva-Magalhães Talita Dias , de Mello Monteiro Carlos Bandeira
Published: September 2024

Background/Objectives: Fibromyalgia (FM) is a chronic condition characterized by widespread musculoskeletal pain, fatigue, and impaired motor performance. This study aimed to investigate the effects of transcranial direct current stimulation (tDCS) during virtual reality (VR) tasks on the motor performance of women with FM. Methods: Participants were divided into two groups: Group A received active tDCS for 10 days followed by sham tDCS for 10 days, while Group B received the opposite sequence. Both groups performed VR tasks using MoveHero software (v. 2.4) during the tDCS sessions. Motor performance was assessed by the number of hits (movement with correct timing to reach the targets) and absolute (accuracy measure) and variable (precision measure) errors during VR tasks. Participants were 21 women, aged 30–50 years, and diagnosed with FM. Results: Group A, which received active tDCS first, presented significant improvements in motor performance (number of hits and absolute and variable errors). The benefits of active tDCS persisted into the sham phase, suggesting a lasting neuroplastic effect. Conclusions: tDCS during VR tasks significantly improved motor performance in women with FM, particularly in complex, extensive movements. These findings indicate that tDCS enhances neuroplasticity, leading to sustained motor improvements, making it a promising therapeutic tool in FM rehabilitation.
Understanding the Emergence of Comorbidity between Problematic Online Gaming and Gambling: A Network Analysis Approach
Authors: Błoch Marta , Misiak Błażej
Published: September 2024

Background/Objectives: Problematic online gaming and gambling tend to co-occur. The exact mechanisms underlying this phenomenon and the potential effects of gender differences remain unknown. This study aimed to identify the early clustering patterns of problematic online gaming and gambling in a community sample of young adults without a lifetime history of psychiatric treatment. Methods: Data were collected through an online survey and analyzed using partial correlations and Bayesian networks. Results: Altogether, 1441 individuals (aged 18–40 years, 51.4% females) participated in the survey. Both problematic online behaviors were weakly interrelated, suggesting that they serve as distinct constructs. Men’s networks appeared to be more complex and had significantly higher global connectivity. Moreover, men and women differed with respect to the specific nodes that bridged both constructs. In men, the bridge nodes were “being criticized because of betting or being told about gambling problems”, “loss of previous interests due to gaming”, “deceiving other people because of gaming”, and “health consequences of gambling”. Among women, the bridge nodes were “feeling guilty because of gambling”, “loss of previous interests because of gaming”, “social consequences of gaming”, and “continued gaming problems with other people”. In men, the strongest edge was found between “borrowing money/selling anything to gamble” and “financial problems because of gambling”, while in women, the strongest edge appeared between “betting more than afforded to be lost” and “tolerance symptoms of gambling”. Conclusions: The findings indicate that problematic online gaming and gambling tend to emerge in different ways among men and women. Therapeutic interventions should be planned considering gender differences.
Exploring Sleep Architecture in Polish Patients with Multiple Sclerosis: A Polysomnography Study
Authors: Rzepka Michalina , Chmiela Tomasz , Galus Weronika , Lasek-Bal Anetta , Krzystanek Ewa
Published: September 2024

Background: Sleep disturbances are a prevalent phenomenon in patients with multiple sclerosis (PwMS). The present study employs polysomnography (PSG) to quantify sleep efficiency and architecture in PwMS, aiming to elucidate the relationships between PSG parameters and factors including gender, disability level, brain lesion location, and subjective measures of insomnia, excessive daytime sleepiness (EDS), fatigue, pain, and mood disorders. Methods: The study cohort comprised 51 adult PwMS, of whom 31 underwent overnight PSG. The demographic and clinical characteristics, including age, gender, and Expanded Disability Status Scale (EDSS), were collated. The Athens Insomnia Scale, the Epworth Sleepiness Scale, the Fatigue Severity Scale, the Modified Fatigue Impact Scale (MFIS), the Numerical Pain Rating Scale, and the Hospital Anxiety and Depression Scale were employed for the assessment of insomnia, EDS, fatigue, pain, and mood disorders. The brain and spinal cord magnetic resonance imaging (MRI) were evaluated. Results: A reduced sleep efficiency was observed among 30 PwMS (aged 38.9 ± 12.9), with a mean of 80 ± 12%, especially in those with brainstem demyelinating lesions. In those PwMS aberrant sleep onset latency (SOL) and wake after sleep onset were also noted (p < 0.05). The prevalence of sleep fragmentation, as measured by the total arousal index, was greater in male PwMS than in female (p < 0.05). Higher disability according to the EDSS correlated with longer SOL (ρ = 0.48, p < 0.05), and reduced N2 sleep stage correlated with cognitive fatigue according to MFIS (ρ = −0.46, p < 0.05). Age, disease duration, insomnia, EDS, physical fatigue, and mood disorders did not impact PSG parameters. Conclusions: The study demonstrated the disruption of sleep architecture in PwMS, and highlighted the importance of a comprehensive PSG assessment of sleep disturbances in this population.
Correction: Amin et al. Hippocampal and Cerebellar Changes in Acute Restraint Stress and the Impact of Pretreatment with Ceftriaxone. Brain Sci. 2020, 10, 193
Authors: Amin Shaimaa N. , Hassan Sherif S. , Khashaba Ahmed S. , Youakim Magdy F. , Latif Noha S. Abdel , Rashed Laila A. , Yassa Hanan D.
Published: September 2024

In the original publication [...]
The Impact of Close Reading Strategies on Individual Innovativeness and Life Skills: Preservice Teachers
Authors: Baki Yasemin
Published: September 2024

Reading is one of the fundamental tools for acquiring knowledge that has a direct effect on an individual’s life. Many single and multiple strategies are used to improve reading comprehension, which is a strategic acquisition. (1) This study aims to investigate the effects of close reading strategies on the life skills and individual innovativeness of preservice Turkish language teachers. (2) The study group for this research consists of 31 preservice teachers studying in the Turkish Language Teaching department at a university in northern Türkiye. A sequential mixed-method design was used. In the quantitative aspect of the research, a pre-test and post-test experimental design without a control group was used, while in the qualitative aspect, a case study design was employed. Data were collected using the Life Skills Scale, Individual Innovativeness Scale, and semi-structured interview form. In analyzing the data obtained to examine the effect of the experimental process, a t-test for dependent groups was used, whereas data from the interviews were analyzed through content analysis (3–4). According to the results obtained from the research, the close reading strategy was determined to be a strategy that significantly improves the life skills and individual innovativeness of preservice Turkish language teachers.
The Relationship between Laissez-Faire Leadership and Cyberbullying at Work: The Role of Interpersonal Conflicts
Authors: Cárdenas-Miyar Alfonso , Cantero-Sánchez Francisco , León-Rubio José , Orgambídez-Ramos Alejandro , León-Pérez Jose
Published: September 2024

A person can experience cyberbullying at work when exposed to repetitive and intrusive negative acts facilitated by new information and communication technologies (ICTs). The incidence of workplace cyberbullying has rapidly increased following the COVID-19 pandemic. This issue does not arise in isolation; leadership plays a critical role. Leaders who fail to set clear rules and provide minimal supervision may exacerbate interpersonal conflicts among subordinates. This study explores the role of laissez-faire leadership and interpersonal conflicts on workplace cyberbullying from a gender perspective. A two-wave panel study was conducted (N = 1995; 53.6% women; M age = 42.02 years old; SD = 9.23; age range: 18–74 years old). Our findings indicate no direct relationship between laissez-faire leadership and workplace cyberbullying; however, there is a significant indirect relationship. Laissez-faire leadership is associated with a higher frequency of interpersonal conflicts, which in turn are related to cyberbullying, making interpersonal conflicts a mediator. Additionally, gender moderates the relationship between interpersonal conflicts and workplace cyberbullying. Our results suggest that interpersonal conflicts may increase exposure to cyberbullying, particularly for men under laissez-faire leadership. These findings have managerial implications for introducing tailored interventions to prevent workplace cyberbullying.
Music Listening as Exploratory Behavior: From Dispositional Reactions to Epistemic Interactions with the Sonic World
Authors: Reybrouck Mark , Podlipniak Piotr , Welch David
Published: September 2024

Listening to music can span a continuum from passive consumption to active exploration, relying on processes of coping with the sounds as well as higher-level processes of sense-making. Revolving around the major questions of “what” and “how” to explore, this paper takes a naturalistic stance toward music listening, providing tools to objectively describe the underlying mechanisms of musical sense-making by weakening the distinction between music and non-music. Starting from a non-exclusionary conception of “coping” with the sounds, it stresses the exploratory approach of treating music as a sound environment to be discovered by an attentive listener. Exploratory listening, in this view, is an open-minded and active process, not dependent on simply recalling pre-existing knowledge or information that reduces cognitive processing efforts but having a high cognitive load due to the need for highly focused attention and perceptual readiness. Music, explored in this way, is valued for its complexity, surprisingness, novelty, incongruity, puzzlingness, and patterns, relying on processes of selection, differentiation, discrimination, and identification.
Bond Behavior of CFRP–Concrete Bonded Joints with Additional GFRP Layer: Effect of Bonding Sequence
Authors: Zhou Hao , Zhao Jiahao , Yang Yan , Tan Fengling , Ou Ya , Wang Yi , Li Chao
Published: September 2024

Existing studies have shown that bonding a ±45° biaxial GFRP under CFRP laminate can significantly improve the load-carrying capacity and ultimate deformation of CFRP–concrete bonded joints. In such a bonding configuration, the GFRP interlayer is wider than the CFRP laminate so that the interfacial stress can be redistributed to achieve a higher fracture work; however, the effect of the bonding sequence,—specifically, the position of the GFRP layer—on the bond behavior is not yet clear. In this study, considering the same CFRP and GFRP usage, three types of CFRP–concrete bonded joints with CFRP bonded under, above, and between GFRP layers were prepared and tested under single-shear loading. Digital image correlation (DIC) was used to measure the deformation of the bonded joints during the test. Afterward, the failure mode, load–displacement behavior, and principal strain distribution were analyzed. The experimental results show that the dominant failure mode is the combined cohesion failure mode within the concrete and GFRP delamination, which is not affected by the bonding sequence. Compared to conventional CFRP–concrete bonded joints, bonding the CFRP laminate above, under, and between the GFRP layers achieved a 157.6%, 175.0%, and 177.2% increase in load-carrying capacity, respectively. Accordingly, the ultimate deformation also recorded an 83.0%, 103.6%, and 86.3% increase. However, the bonding sequence showed a slight influence on the initial stiffness of the load–displacement curve with a maximum difference of 16.1%, taking the minimum as a reference, which could be attributed to the differences in the strength and stiffness between the CFRP–concrete and CFRP–GFRP–concrete interfaces.
The Role of Water Bodies in Climate Regulation: Insights from Recent Studies on Urban Heat Island Mitigation
Authors: Jandaghian Zahra , Colombo Andrew
Published: September 2024

Urban heat islands (UHIs) pose a significant challenge in cities worldwide, exacerbating energy use, air pollution, and health risks. This paper reviews the role of water bodies in mitigating UHI effects, which is vital for informed urban planning and climate adaptation. We analyze how water features, particularly when combined with green spaces and strategic urban design, can significantly cool urban environments. The effectiveness of water bodies in reducing temperatures is influenced by their size, shape, surrounding land use, climatic conditions, and vegetation. Empirical research and case studies indicate that larger and well-shaped water bodies, due to their extensive surface area and continuous evaporation, are more effective. Furthermore, the integration of water bodies with green spaces enhances cooling through increased evapotranspiration and shading. This review highlights the strategic placement and design of water bodies within urban landscapes as crucial for maximizing their cooling benefits. By integrating water features with other urban cooling strategies, such as tree planting and expanded greenery, cities can effectively counter UHI effects, leading to more sustainable and resilient urban environments.
Basic Mechanical Properties of Self-Compacting Concrete Prepared with Aeolian Sand and Recycled Coarse Aggregate
Authors: Zheng Shiqi , Liu Qing , Han Fengxia , Liu Shan , Han Tong , Yan Hao
Published: September 2024

To protect the environment and reduce the consumption of natural resources, this study investigated the performance of self-compacting concrete (SCC) utilizing aeolian sand (AS) as a replacement for fine aggregate and recycled coarse aggregate (RCA) as a replacement for natural coarse aggregate. Twelve mixing ratios were prepared, with AS replacement ratios at 0, 20%, 40%, and 60% and RCA replacement ratios at 0, 25%, and 50%. The evaluation primarily focused on workability, uniaxial compressive strength, split tensile strength, stress–strain curve, modulus of elasticity, and axial compressive strength. The results indicated that both AS and RCA can enhance the performance of SCC at appropriate replacement ratios, and a 20% AS and 50% RCA replacement ratio significantly improved the performance of SCC. In addition, the effects of AS and RCA replacements on SCC were evaluated by several performance indexes, which provides a basis for subsequent experimental studies and demonstrates the feasibility of incorporating AS and RCA into SCC.
The Economic Impact of Treatment Sequencing in Chronic Lymphocytic Leukemia in Canada Using Venetoclax plus Obinutuzumab
Authors: Guinan Kimberly , Mathurin Karine , Lachaine Jean , Roc Nancy Paul , Bull Sarah-Jane , Tankala Dipti , Barakat Stephane , Manzoor Beenish S. , Hillis Christopher , Banerji Versha
Published: September 2024

Background: Bruton tyrosine kinase inhibitors (BTKis) represent an advancement in chronic lymphocytic leukemia; however, these agents are administered continuously until disease progression or unacceptable toxicity, raising concerns about their affordability. Venetoclax in combination with obinutuzumab (VO) is a fixed-duration (12-month) treatment, approved in Canada in 2020. This study estimated the total cumulative cost of different treatment sequences and evaluated the economic impact of introducing treatment sequences with/without VO, from a Canadian health care system perspective. Methods: A 10-year partitioned survival model was developed, considering key clinical parameters and direct medical costs. Results were stratified by TP53 aberration. Results: Treatment sequences starting with first-line (1L) VO resulted in lower 10-year cumulative costs compared to sequences starting with BTKis administered until disease progression, across both TP53 aberration subgroups. With a maximum of three lines of treatment over a 10-year period, cumulative costs were largely determined by the first two lines of treatment. When comparing sequences with the same 1L treatment, sequences with BTKis in second-line incurred greater costs compared to fixed-duration regimens. Conclusions: Overall, the economic impact of treating all patients with VO led to 10-year cumulative savings of CAD 169,341 and CAD 293,731 per patient, without and with TP53 aberration, respectively. These savings are mainly due to reductions in treatment costs associated with fixed treatment duration.
Correction: Huang et al. Endogenous Propionibacterium acnes Promotes Ovarian Cancer Progression via Regulating Hedgehog Signalling Pathway. Cancers 2022, 14, 5178
Authors: Huang Qifa , Wei Xin , Li Wenyu , Ma Yanbing , Chen Guanxiang , Zhao Lu , Jiang Ying , Xie Siqi , Chen Qi , Chen Tingtao
Published: September 2024

In the original publication [...]
Influence of Current Collector Design and Combination on the Performance of Passive Direct Methanol Fuel Cells
Authors: Yu Weibin , Xiao Zhiyuan , Zhang Weiqi , Ma Qiang , Li Zhuo , Yan Xiaohui , Su Huaneng , Xing Lei , Xu Qian
Published: September 2024

In this work, an anode current collector with a scaled step-hole structure (called SF-type) and a cathode current collector with a perforated cross-tilt structure (called X-type) were designed and fabricated for application in passive direct methanol fuel cells (DMFCs). A whole-cell test showed that the combination of an anode SF-type current collector and cathode conventional current collector increased the optimal methanol concentration from 6 M to 8 M and the maximum power density to 5.40 mW cm−2, which improved the cell performance by 51.6% compared to that of the conventional design under ambient testing conditions. The combination of the anode conventional current collector and cathode X-type current collector achieved a maximum power density of 5.65 mW cm−2 with a 58.7% performance improvement, while the optimal methanol concentration was increased to 10 M. Furthermore, the combination of anode SF-type and cathode X-type obtained the highest power density at 6.22 mW cm−2. Notably, the anode and cathode catalyst loadings used in this study were 2.0 mg cm−2, which is lower than the commonly used loading, thus reducing the fuel cell cost.
Circulating Exosomal miRNA Profiles in Non-Small Cell Lung Cancers
Authors: Hassanin Abeer A. I. , Ramos Kenneth S.
Published: September 2024

A growing number of studies have shown that microRNAs (miRNAs) can exert oncogenic or tumor suppressor activities in a variety of cancers, including lung cancer. Given their presence in exosome preparations, microRNA molecules may in fact participate in exosomal intercellular transfers and signaling. In the present study, we examined the profile of 25 circulating exosomal microRNAs in ostensibly healthy controls compared to patients with squamous cell lung cancers (SQCLC) or lung adenocarcinomas (LUAD). Eight miRNAs, namely, miR-21-5p, miR-126-3p, miR-210-3p, miR-221-3p, Let-7b-5p, miR-146a-5p, miR-222-3p, and miR-9-5p, were highly enriched in the cohort and selected for further analyses. All miRNAs were readily detected in non-small cell lung cancer (NSCLC) patients of both sexes at all cancer stages, and their levels in exosomes correlated with the clinicopathological characteristics of tumors. Thus, the presence of these miRNAs in circulating exosomes may contribute to the regulation of oncogenic activity in patients with NSCLC.
Comparative Analysis of Inhibitory and Activating Immune Checkpoints PD-1, PD-L1, CD28, and CD86 in Non-Melanoma Skin Cancer
Authors: Winter Linus , Ries Jutta , Vogl Christoph , Trumet Leah , Geppert Carol Immanuel , Lutz Rainer , Kesting Marco , Weber Manuel
Published: September 2024

The establishment of immunotherapy applying immune checkpoint inhibitors (ICI) has provided an important new option for the treatment of solid malignant diseases. However, different tumor entities show dramatically different responses to this therapy. BCC responds worse to anti-PD-1 ICIs as compared to cSCC. Differential immune checkpoint expression could explain this discrepancy and, therefore, the aim of this study was to analyze activating and inhibitory immune checkpoints in cSCC and BCC tissues. Tissue microarrays of the invasive front as well as the tumor core of BCC and cSCC samples were used to evaluate PD-1, PD-L1, CD28, and CD86 expression and their topographic distribution profiles by chromogenic immunohistochemistry. QuPath was used to determine the labeling index. The expression of PD-1, PD-L1, and CD28 was significantly higher in both the tumor core and the invasive front of cSCC samples as compared to BCC (p < 0.001). In addition, the ratios of PD-L1/CD86 (p < 0.001) and CD28/CD86 (p < 0.001) were significantly higher in cSCC. The invasive front of both tumor entities showed higher expression levels of all immune markers compared to the tumor core in both tumor entities. The significantly higher expression of PD-1, PD-L1, and CD28 in cSCC, along with the predominance of the inhibitory ligand PD-L1 as compared to the activating CD86 in cSCC, provide a potential explanation for the better objective response rates to anti-PD-1 immunotherapy as compared to BCC. Furthermore, the predominant site of interaction between the immune system and the tumor was within the invasive front in both tumor types.
Effect of Inorganic Anions on the Structure of Alkali-Activated Blast Furnace Slag
Authors: Świszcz Dominika , Marzec Mateusz , Mozgawa Włodzimierz , Król Magdalena
Published: September 2024

Analyzing the effect of anions on the structure of geopolymers is crucial because anions can significantly influence the material’s chemical stability, mechanical properties, and long-term durability. Understanding these effects helps optimize geopolymer compositions for various applications, such as construction materials and waste encapsulation. This research report describes the effects of nitrate, sulfate, and phosphate anions on alkali-activated blast furnace slag’s structural integrity and properties. Advanced techniques like XRD, FT-IR, Raman spectroscopy, and XPS have been employed to analyze structural modifications caused by anions, providing insights into their interactions and effects. These anions generally decrease compressive strength by disrupting geopolymerization and altering microstructure. For example, sulfate ions lead to the formation of ettringite, while phosphate ions bind calcium into separate phases. We can also observe microstructural changes, such as increased porosity with phosphate, which significantly reduces strength. Nitrate’s effect is less detrimental but still influences the overall structural dynamics.
Influence of Metal Ions on the Structural Complexity of Mixed-Ligand Divalent Coordination Polymers
Authors: Cheng Fang-Ju , Wang Kai-Min , Lee Chia-Yi , Wang Song-Wei , Thapa Kedar Bahadur , Govindaraj Manivannan , Chen Jhy-Der
Published: September 2024

The reactions of the angular ligand 4,4′-oxybis(N-(pyridin-3-yl)benzamide) (L1) and 1,4-naphthalenedicarboxylic acid (1,4-H2NDC) with divalent metal salts yielded three distinct coordination polymers (CPs): {[Zn2(L1)(1,4-NDC)2]·MeOH}n, 1, {[Cu(L1)(1,4-NDC)(H2O)]·3H2O}n, 2, and {[Cd(L1)(1,4-NDC)]·2H2O}n, 3. Complex 1 features a 2-fold interpenetrated 3D framework with the (412·63)-pcu topology, while complex 2 reveals a 1D triple-strained helical chain and complex 3 displays a 3-fold interpenetrated 3D framework with (66)-dia topology. Additionally, the reactions of the flexible ligand N,N′-bis(3-methylpyridyl) adipoamide (L2) afforded {[Co4(L2)0.5(1,4-NDC)3(H2O)3(µ3-OH)2]·EtOH·2H2O}n, 4, {[Zn2(L2)(1,4-NDC)2]·2CH3OH}n, 5, and [Cd(L2)(adipic)(H2O)]n (H2adipic = adipic acid), 6, exhibiting a self-catenated 3D framework with the (420·68)-8T32 topology, a 2D layer with the (413·62) − (4,4)IIb topology, and a 2D layer with the (44·62)-sql topology, respectively. The structural diversity observed in complexes 1–6 highlights the pivotal influence of the metal center on the degree of entanglement in CPs within mixed-ligand systems. The thermal stability and luminescent properties of complexes 1–3, 4, and 6 are also discussed.
Advancements in Microfluidic Platforms for Glioblastoma Research
Authors: Raman Rachana , Prabhu Vijendra , Kumar Praveen , Mani Naresh Kumar
Published: September 2024

Glioblastoma (GBM) is a malignant cancer affecting the brain. As per the WHO classifications, it is a grade IV glioma and is characterized by heterogenous histopathology, high recurrence rates, and a high median age of diagnosis. Most individuals diagnosed with GBM are aged between 50 and 64 years, and the prognosis is often poor. Untreated GBM patients have a median survival of 3 months, while treatments with Temozolomide (TMZ) and radiotherapy can improve the survival to 10–14 months. Tumor recurrence is common, owing to the inefficiency of surgical resection in removing microscopic tumor formations in the brain. A crucial component of GBM-related research is understanding the tumor microenvironment (TME) and its characteristics. The various cellular interactions in the TME contribute to the higher occurrence of malignancy, resistance to treatments, and difficulty in tumor resection and preventative care. Incomplete pictures of the TME have been obtained in 2D cultures, which fail to incorporate the ECM and other crucial components. Identifying the hallmarks of the TME and developing ex vivo and in vitro models can help study patient-specific symptoms, assess challenges, and develop courses of treatment in a timely manner which is more efficient than the current methods. Microfluidic models, which incorporate 3D cultures and co-culture models with various channel patterns, are capable of stimulating tumor conditions accurately and provide better responses to therapeutics as would be seen in the patient. This facilitates a more refined understanding of the potential treatment delivery systems, resistance mechanisms, and metastatic pathways. This review collates information on the application of such microfluidics-based systems to analyze the GBM TME and highlights the use of such systems in improving patient care and treatment options.
Wettability of a Polymethylmethacrylate Surface by Fluorocarbon Surfactant Solutions
Authors: Yan Fei , Ma Cheng , Gong Qingtao , Jin Zhiqiang , Ma Wangjing , Xu Zhicheng , Zhang Lei , Zhang Lu
Published: September 2024

To clarify the adsorption behavior of fluorocarbon surfactants on PMMA surfaces, the contact angles of two nonionic fluorocarbon surfactants (FNS-1 and FNS-2) and an anionic fluorocarbon surfactant (FAS) on polymethylmethacrylate (PMMA) surface were determined using the sessile drop method. Moreover, the effects of molecular structures on the surface tension, adhesion tension, solid–liquid interfacial tension, and adhesion work of the three fluorocarbon surfactants were investigated. The results demonstrate that the adsorption amounts for three fluorocarbon surfactants at the air–water interface are 4~5 times higher than those at the PMMA–solution interface. The three fluorocarbon surfactants adsorb on the PMMA surface by polar groups before CMC and by hydrophobic chains after CMC. Before CMC, FNS-2 with the smallest molecular size owns the highest adsorption amount, while FAS with large-branched chains and electrostatic repulsion has the smallest adsorption amount. After CMC, the three fluorocarbon surfactants form aggregates at the PMMA-liquid interface. FAS possesses the smallest adsorption amount after CMC. Besides, FNS-1 possesses a higher adsorption amount than FNS-2 due to the longer fluorocarbon chain and the lower CMC value of FNS-1. The adsorption behaviors of nonionic and anionic fluorocarbon surfactants on the PMMA surface are different. FAS forms interfacial aggregates before CMC, which may be attributed to the electrostatic interaction between the anionic head of FAS and the PMMA surface.
Enhancing Deep Excavation Optimization: Selection of an Appropriate Constitutive Model
Authors: Dahal Bhim Kumar , Regmi Sandip , Paudyal Kalyan , Dahal Diwash , KC Diwakar
Published: September 2024

To minimize the impact on nearby structures during deep excavations, choosing an appropriate soil constitutive model for analysis holds significant importance. This study aims to conduct a comparative analysis of various constitutive soil models—namely, the Mohr–Coulomb (MC) model, the hardening soil (HS) model, the hardening soil small strain (HSS) model, and the soft soil (SS) model—to identify the most suitable model for the lacustrine deposit. To implement these models, the soil’s index properties and mechanical behavior were evaluated from undisturbed soil samples. The numerical simulation and verification of these properties were carried out by comparing the laboratory test results with the outcome of the finite element method; the most suitable constitutive soil model for the soil was identified as the HSS model. Upon analyzing the wall deflection and ground settlement profiles obtained from respective constitutive models, it was observed that the HS and HSS models exhibit similar characteristics and are well-suited for analyzing typical lacustrine soil. In contrast, the MC and SS models yield overly optimistic results with lower wall deflection and ground settlement and fail to predict realistic soil behavior. As a result, this research highlights the significance of selecting the appropriate constitutive soil model and refining the parameters. This optimization process contributes significantly to the design of support systems, enhancing construction efficiency and ensuring overall safety in deep excavation projects.
The Role of COVID-19 Vaccination in Serological and Infectious Response in the Xokós Indigenous Community
Authors: Silva Eloia Emanuelly Dias , Barreto Marina dos Santos , Santos Ronaldy Santana , Silva Deise Maria Rego Rodrigues , Moura Pedro Henrique Macedo , de Jesus Pamela Chaves , de Souza Jessiane Bispo , Guimarães Adriana Gibara , Santana Lucas Alves da Mota , Borges Lysandro Pinto
Published: September 2024

Objectives: This study aims to examine the serological and infectious characteristics of the Xokós indigenous community in Brazil, both prior to and following COVID-19 immunization; Methods: Immunofluorescence assays were employed to identify the SARS-CoV-2 viral antigen, while IgM and IgG antibody tests for COVID-19 were utilized to assess the participants’ infectious and serological profiles in July 2020, before the commencement of the COVID-19 vaccination campaign, and in March 2022, during the booster dose vaccination campaign; Results: The majority of participants (n = 22) were female, with an average age of 42.20 years. The most prevalent comorbidity was hypertension (60%; n = 9), followed by hypertension associated with diabetes (20%; n = 3). No statistically significant correlation was found between the timing of vaccination and the levels of antigens or IgM. However, the prevalence of reactive antigens and IgM was 13.3% (n = 4) in the pre-vaccination group and 3.3% (n = 1) in the post-vaccination group. A statistically significant difference in IgG production was observed before and after vaccination (χ2(1) = 39.095, p < 0.01), as well as differences in IgG antibody detection before and after vaccination and in the vaccines used. Participants showed a higher probability of reactive IgG antibodies following vaccination; Conclusions: Our data demonstrate the beneficial effects of vaccination on the indigenous community, highlighting that continued immunization is a crucial step in protecting indigenous health and preventing severe outbreaks and deaths associated with the disease.
CHAM-CLAS: A Certificateless Aggregate Signature Scheme with Chameleon Hashing-Based Identity Authentication for VANETs
Authors: Kabil Ahmad , Aslan Heba , Azer Marianne A. , Rasslan Mohamed
Published: September 2024

Vehicular ad hoc networks (VANETs), which are the backbone of intelligent transportation systems (ITSs), facilitate critical data exchanges between vehicles. This necessitates secure transmission, which requires guarantees of message availability, integrity, source authenticity, and user privacy. Moreover, the traceability of network participants is essential as it deters malicious actors and allows lawful authorities to identify message senders for accountability. This introduces a challenge: balancing privacy with traceability. Conditional privacy-preserving authentication (CPPA) schemes are designed to mitigate this conflict. CPPA schemes utilize cryptographic protocols, including certificate-based schemes, group signatures, identity-based schemes, and certificateless schemes. Due to the critical time constraints in VANETs, efficient batch verification techniques are crucial. Combining certificateless schemes with batch verification leads to certificateless aggregate signature (CLAS) schemes. In this paper, cryptanalysis of Xiong’s CLAS scheme revealed its vulnerabilities to partial key replacement and identity replacement attacks, alongside mathematical errors in the batch verification process. Our proposed CLAS scheme remedies these issues by incorporating an identity authentication module that leverages chameleon hashing within elliptic curve cryptography (CHAM-CLAS). The signature and verification modules are also redesigned to address the identified vulnerabilities in Xiong’s scheme. Additionally, we implemented the small exponents test within the batch verification module to achieve Type III security. While this enhances security, it introduces a slight performance trade-off. Our scheme has been subjected to formal security and performance analyses to ensure robustness.
The Counterion (SO42− and NO3−) Effect on Crystallographic, Quantum-Chemical, Protein-, and DNA-Binding Properties of Two Novel Copper(II)–Pyridoxal-Aminoguanidine Complexes
Authors: Jevtovic Violeta , Golubović Luka , Alshammari Odeh A. O. , Alhar Munirah Sulaiman , Alanazi Tahani Y. A. , Rakic Violeta , Ganguly Rakesh , Dimitrić Marković Jasmina , Rakić Aleksandra , Dimić Dušan
Published: September 2024

New Cu(II) complexes with pyridoxal-aminoguanidine (PLAG) ligands and different counterions (SO42− and NO3−) were prepared and their crystal structures were solved by the X-ray crystallography. The geometries of the obtained complexes significantly depended on the counterions, leading to the square-pyramidal structure of [Cu(PLAG)NO3H2O]NO3 (complex 1) and square-planar structure of [Cu(PLAG)H2O]SO4 (complex 2). The intermolecular interactions were examined using the Hirshfeld surface analysis. The theoretical structures of these complexes were obtained by optimization at the B3LYP/6-311++G(d,p)(H,C,N,O,S)/LanL2DZ(Cu) level of theory. The Quantum Theory of Atoms in Molecules (QTAIM) was applied to assess the strength and type of the intramolecular interactions and the overall stability of the structures. The interactions between the complexes and transport proteins (human serum albumin (HSA)) and calf thymus DNA (CT-DNA) were examined by spectrofluorometric/spectrophotometric titration and molecular docking. The binding mechanism to DNA was assessed by potassium iodide quenching experiments. The importance of counterions for binding was shown by comparing the experimental and theoretical results and the examination of binding at the molecular level.
The Effects of Arbuscular Mycorrhiza Trees on the Diversity of Forest Communities Worldwide Are Greater than Those of Ectotrophic Mycorrhiza Trees
Authors: Li Zihao , Liu Wenxin , Xue Xinyu , Qi Rui , Li Xueying , Li Qian , Xu Nuonuo , Liu Fengqin , Shao Yizhen , Ye Yongzhong , Chen Yun , Wei Dongwei , Yuan Zhiliang
Published: September 2024

The interaction between woody plants and mycorrhizal fungi is an important biological interaction; however, the driving factors behind the diversity of mycorrhizal trees formed through the symbiosis of mycorrhizal fungi and woody plants remain unclear. In this study, we collected and compiled the woody plant data of 34 forest dynamic plots containing 3350 species from habitats around the world and divided them into AM and EcM trees. We tested the contribution of AM and EcM trees to tree diversity and its components in forest communities worldwide. Our results showed that AM trees rather than EcM trees affect the tree diversity of forest communities, and that the diversity of AM trees has a significant latitudinal gradient pattern. Climate variables, especially temperature, are strongly correlated with the diversity patterns for AM trees rather than EcM trees. Topography is the most significant factor affecting the diversity of EcM trees. Our findings highlight the importance of AM trees for the tree diversity of forest communities worldwide. Our findings have important implications for understanding the response of complex woody plant communities with different types of mycorrhizal symbiosis to climate change.
Genetic Diversity, Kinship, and Polychromatism in the Spotted Eagle Ray Aetobatus ocellatus of Fiji
Authors: Glaus Kerstin , Appleyard Sharon A.
Published: September 2024

The spotted eagle ray Aetobatus ocellatus (Kuhl, 1923) has a widespread Indo-West Pacific distribution and displays substantial population genetic structuring. Genetic data are crucial for understanding the species’ diversity, connectivity, and adaptation. However, molecular genetic information on A. ocellatus from Melanesia is lacking, which impedes our understanding of gene flow among geographic regions. In this study, we sampled 45 A. ocellatus, primarily from Fiji’s largest fish market in the capital, Suva. Mitochondrial DNA Cytochrome C Oxidase subunit I (COI) barcoding was used for species identification, and DArT-seqTM technology was applied to assess the nuclear genetic diversity. Barcoding of the COI gene showed a 98.6% to 99.8% similarity to A. ocellatus reference sequences in the Barcode of Life Data System, and the 45 individuals were represented by three major evolutionary haplotype clusters. Genotyping resulted in 24,313 Single-Nucleotide Polymorphisms (SNPs) which were quality-filtered to 7094 SNPs per individual. The observed heterozygosity level was 0.310. The inbreeding coefficient was positive, and genotyping identified one full-sibling pair and one half-sibling pair from the 45 individuals. Additionally, eagle rays exhibit polychromatic patterns, and at least three ventral pattern variations were recorded in specimens from the market. Collectively, our main findings characterize the genetic profile of A. ocellatus in Fiji and can help to understand the diversification of this species within the region.
Diversity of Rotifera in Freshwaters of Bolivia: An Updated Checklist
Authors: Fernández Carla E. , Campero Melina , Acosta Francisca , Prado Pablo E. , Maldonado Mabel , Goitia Edgar , Stamou Georgia , Michaloudi Evangelia , López Carlos
Published: September 2024

Biodiversity records are of great importance, especially in light of the biodiversity crisis. Here, we present a study on the diversity of rotifers in Bolivia based on an extensive investigation of the literature published so far. Through this approach and an analysis of samples from 207 water bodies of the country, we updated the checklist of reported species. This study revealed a total of 195 species of rotifers previously reported; we identified 153 species in our samples, with 84 of them being reported for the first time in Bolivia. Thus, a total of 279 species are known at present in this country. Our findings suggest that Bolivia has a rich and diverse rotifer community, with many species likely to be unique to the region.
Control of the Properties of the Voronoi Tessellation Technique and Biomimetic Patterns: A Review
Authors: Arvizu Alonso Ana Karilú , Armendáriz Mireles Eddie Nahúm , Calles Arriaga Carlos Adrián , Rocha Rangel Enrique
Published: September 2024

The cellular behavior of Voronoi tessellation has generated interest due to its applicability in various fields and its notable structural properties. Controlling factors such as the gradient of the cells, the position of seed points, and the thickness of the arms allows for adjusting rigidity and flexibility according to specific needs. This article analyzes the state of the art of this technique, exploring its modification for applications in engineering and design, complemented with information on natural structural properties. This comprehensive analysis provides a complete overview of Voronoi tessellation and its potential in engineering and design, categorizing methodologies according to selected processing methods and highlighting techniques for altering structural behavior. Additionally, it emphasizes the integration of biomimetic approaches, connecting nature with technology to foster continuous innovation. Finally, this article addresses encountered limitations, offering future perspectives for the cellular technique and highlights the complexity of reproducibility due to reserved or generalized steps, despite the significant diversity in implemented techniques.
Assessing the Predictive Utility of the C-Reactive Protein-to-Lymphocyte Ratio for Mortality in Isolated Traumatic Brain Injury: A Single-Center Retrospective Analysis
Authors: Huang Ching-Ya , Wu Shao-Chun , Yen Yuan-Hao , Yang Johnson Chia-Shen , Hsu Shiun-Yuan , Hsieh Ching-Hua
Published: September 2024

Introduction: Early identification of high-risk traumatic brain injury (TBI) patients is crucial for optimizing treatment strategies and improving outcomes. The C-reactive protein-to-lymphocyte ratio (CLR) reflects systemic immunology and inflammation function and serves as a new biomarker for patient stratification. This study aimed to assess the predictive value of the CLR for mortality in patients with isolated moderate to severe TBI. Methods: A retrospective analysis of trauma registry data from 2009 to 2022 was conducted, including 1641 adult patients with isolated moderate to severe TBI. Patient demographics, the CLR, injury characteristics, and outcomes were compared between deceased and surviving patients. Univariate and multivariate analyses were performed to identify mortality risk factors. The optimal CLR cut-off value for predicting mortality was determined using receiver operating characteristic (ROC) curve analysis. Results: The CLR was significantly higher in deceased patients compared to survivors (60.1 vs. 33.9, p < 0.001). The optimal CLR cut-off value for predicting mortality was 54.5, with a sensitivity of 0.328 and a specificity of 0.812. The area under the ROC curve was 0.566, indicating poor discriminative ability. In the multivariate analysis, the CLR was not a significant independent predictor of mortality (OR 1.03, p = 0.051). After propensity score matching to attenuate the difference in baseline characteristics, including sex, age, comorbidities, conscious level, and injury severity, the high-CLR group (CLR ≥ 54.5) did not have significantly higher mortality compared to the low-CLR group (CLR < 54.5). Conclusion: While the CLR was associated with mortality in TBI patients, it demonstrated poor discriminative ability as a standalone predictor. The association between a high CLR and worse outcomes may be primarily due to other baseline patient and injury characteristics, rather than the CLR itself.