bluuebunny/crossref_metadata_2025 huggingface dataset:
Inferring HIV Transmission Network Determinants Using Agent-Based Models Calibrated to Multi-Data Sources
Authors: Niyukuri David , Chibawara Trust , Nyasulu Peter Suwirakwenda , Delva Wim
Published: October 2021

(1) Background: Calibration of Simpact Cyan can help to improve estimates related to the transmission dynamics of the Human Immunodeficiency Virus (HIV). Age-mixing patterns in sexual partnerships, onward transmissions, and temporal trends of HIV incidence are determinants which can inform the design of efficient prevention, and linkage-to-care programs. Using an agent-based model (ABM) simulation tool, we investigated, through a simulation study, if estimates of these determinants can be obtained with high accuracy by combining summary features from different data sources. (2) Methods: With specific parameters, we generated the benchmark data, and calibrated the default model in three scenarios based on summary features for comparison. For calibration, we used Latin Hypercube Sampling approach to generate parameter values, and Approximation Bayesian Computation to choose the best fitting ones. In all calibration scenarios the mean square root error was used as a measure to depict the estimates accuracy. (3) Results: The accuracy measure showed relatively no difference between the three scenarios. Moreover, we found that in all scenarios, age and gender strata incidence trends were poorly estimated. (4) Conclusions: Using synthetic benchmarks, we showed that it is possible to infer HIV transmission dynamics using an ABM of HIV transmission. Our results suggest that any type of summary feature provides adequate information to estimate HIV transmission network determinants. However, it is advisable to check the level of accuracy of the estimates of interest using benchmark data.
Ubiquitous Computing: Driving in the Intelligent Environment
Authors: Bran Emanuela , Bautu Elena , Sburlan Dragos Florin , Puchianu Crenguta Madalina , Popovici Dorin Mircea
Published: October 2021

In the context of hyper-connected cars and a growing heterogeneous digital ecosystem, we wish to make the most of the data available from the various sensors, devices and services that compose the ecosystem, in order to propose a proof of concept in-vehicle system that enhances the driving experience. We focus on improving the driving experience along three main directions, namely: (1) driving and trip planning, (2) health and well-being and (3) social and online activities. We approached the in-vehicle space as a smart interface to the intelligent driving environment. The digital data-producers in the ecosystem of the connected car are sources of raw data of various categories, such as data from the outside world, gathered from sensors or online services, data from the car itself and data from the driver gathered with various mobile and wearable devices, by means of observing his state and by means of his social media and online activity. Data is later processed into three information categories—driving, wellness, and social—and used to provide multi-modal interaction, namely visual, audio and gesture. The system is implemented to act in response to the trafficked information on different levels of autonomy, either in a reactive manner, by simple monitoring, or in a proactive manner. The system is designed to provide an in-vehicle system that assists the driver with planning the travel (Drive panel), by providing a comfortable environment for the driver while monitoring him (Wellness panel), and by adaptively managing interactions with their phone and the digital environment (Social panel). Heuristic evaluation of the system is performed, with respect to guidelines formulated for automated vehicles, and a SWOT analysis of the system is also presented in the paper.
Novel Static Multi-Layer Forest Approach and Its Applications
Authors: Bhagwat Ganesh , Kumari Shristi , Patekar Vaishnavi , Deaconu Adrian Marius
Published: October 2021

The existing multi-layer tree is of dynamic linked list type which has many limitations and is complicated due to the pointer-node structure. Static array representation gives more flexibility in programming of algorithms and operations like insertion, deletion, and search. It also reduces the storage space. This paper presents a new method for representing multi-layer forest data structure in array format. It also explains various tree operations, unique data compression algorithm and migration algorithm between traditional approach and the proposed data structure. Most of the fundamental algorithms like those from artificial intelligence that employ decision trees are based on trees/forest data structure. The current paper brings a completely new idea in the representation of these data structures without employing recursion and targeting memory optimizations with reduced code complexities. The applications of forest data structures are many and span over various interdisciplinary areas of Engineering, Medicine, Aviation, Locomotive, Marine, etc. The proposed novel approach not just introduces a new method to look at the tree data structure but also provides the flexibility to adapt to the existing methods as per the user needs. A few such applications in Simulink debugging and the Forest visualisation have been discussed in detail in this paper.
The Speed of Convergence of the Threshold Estimator of Ruin Probability under the Tempered α-Stable Lévy Subordinator
Authors: Gao Yuan , You Honglong
Published: October 2021

In this paper, a nonparametric estimator of ruin probability is introduced in a spectrally negative Lévy process where the jump component is a tempered α-stable subordinator. Given a discrete record of high-frequency data, a threshold technique is proposed to estimate the mean of the jump size and use the Fourier transform and the Pollaczek–Khinchin formula to construct the estimator of ruin probability. The convergence rate of the integrated squared error for the estimator is studied.
Discovery of GOT1 Inhibitors from a Marine-Derived Aspergillus terreus That Act against Pancreatic Ductal Adenocarcinoma
Authors: Yan Shan , Qi Changxing , Song Wei , Xu Qianqian , Gu Lianghu , Sun Weiguang , Zhang Yonghui
Published: October 2021

Pancreatic ductal adenocarcinoma (PDAC) is a devastating digestive system carcinoma with high incidence and death rates. PDAC cells are dependent on the Gln metabolism, which can preferentially utilize glutamic oxaloacetate transaminase 1 (GOT1) to maintain the redox homeostasis of cancer cells. Therefore, small molecule inhibitors targeting GOT1 can be used as a new strategy for developing cancer therapies. In this study, 18 butyrolactone derivatives (1–18) were isolated from a marine-derived Aspergillus terreus, and asperteretone B (5), aspulvinone H (AH, 6), and (+)-3′,3′-di-(dimethylallyl)-butyrolactone II (12) were discovered to possess significant GOT1-inhibitory activities in vitro, with IC50 values of (19.16 ± 0.15), (5.91 ± 0.04), and (26.38 ± 0.1) µM, respectively. Significantly, the molecular mechanism of the crystal structure of GOT1–AH was elucidated, wherein AH and the cofactor pyrido-aldehyde 5-phosphate competitively bound to the active sites of GOT1. More importantly, although the crystal structure of GOT1 has been discovered, the complex structure of GOT1 and its inhibitors has never been obtained, and the crystal structure of GOT1–AH is the first reported complex structure of GOT1/inhibitor. Further in vitro biological study indicated that AH could suppress glutamine metabolism, making PDAC cells sensitive to oxidative stress and inhibiting cell proliferation. More significantly, AH exhibited potent in vivo antitumor activity in an SW1990-cell-induced xenograft model. These findings suggest that AH could be considered as a promising lead molecule for the development of anti-PDAC agents.
Smoking and Obstructive Sleep Apnea: Is There An Association between These Cardiometabolic Risk Factors?—Gender Analysis
Authors: Ioannidou Despoina , Kalamaras George , Kotoulas Serafeim-Chrysovalantis , Pataka Athanasia
Published: October 2021

Background and Objectives: Studies have tried to establish a relationship between Obstructive Sleep Apnea syndrome (OSA) and smoking but data still remain controversial. We aimed: 1. To evaluate the relationship between smoking and OSA; 2. To explore potential differences according to gender, and 3. To analyze the prevalence of cardiovascular disease (CVD) co-morbidities according to gender and smoking status. Materials and Methods: This retrospective study included 3791 (70.6% males) adult patients who visited a Sleep Clinic. All participants underwent nocturnal polysomnography. Daytime somnolence and insomnia were assessed by using the Epworth Sleepiness Scale (ESS) and the Athens Insomnia Scale (AIS). Ever-smokers completed the Fagerstrom Test for Nicotine Dependence (FTND). Results: OSA was confirmed in 72.1% of participants with 62.2% suffering from moderate-to-severe disease. The number of cigarettes/day, Pack/Years, and FTND were significantly higher in patients with more severe OSA. The prevalence of current smokers was higher in those without OSA or with mild disease, whereas the prevalence of former smokers was higher in moderate and severe OSA. In univariate analysis, current smokers were found to be 1.2 times more likely to have OSA compared with never and former smokers combined and former smokers 1.49 times more likely compared with never smokers. In the multiple regression analysis, after adjusting for BMI, gender, age and number of alcoholic drinks per week, smoking was not found to be significantly associated with OSA. In gender stratified multivariate analyses, no significant associations were observed. CVD co-morbidities were more frequent in more severe OSA. Hypertension, coronary disease and diabetes were more prevalent in former smokers with AHI ≥ 15, compared with current smokers, especially in men. Conclusions: Even if an independent effect of smoking on OSA was not found, the number of cigarettes/day, Pack/Years, and FTND were higher in patients with more severe OSA with more prevalent CVD co-morbidities.
Clinical Outcome of Patients Submitted to Liver Resection in the Context of Metastatic Breast Cancer: A Study of a Tertiary Hospital Center
Authors: Nogueiro Jorge , Devezas Vitor , Sousa Fabiana , Fernandes Cristina , Osório Fernando , Costa Susy , Magalhães André , Mora Henrique , Gonçalves Diana , Santos-Sousa Hugo , Pinho André Costa , Graça Luís , Fougo José Luís , Barbosa Elisabete
Published: October 2021

Introduction: Breast cancer is the most incident cancer in the world, accounting for 25% of new cancers per year in females. It is the most frequent malignancy in women, being the fifth cause of death from cancer worldwide. Approximately 5 to 10% of patients already present with metastases at diagnosis, and the liver is the site of metastases in half of these cases. Liver metastasis (LM) resection, performed after neoadjuvant systemic treatment, has been reported to increase median overall survival in this population. Aim: The aim of this analysis is to assess the outcomes of patients undergoing breast cancer liver metastasis surgical resection, including impact on survival, compared to patients where metastasectomy was not performed. Methods: retrospective review of 55 female patients with breast cancer liver metastases, diagnosed and treated in a single tertiary university hospital from January 2011 to December 2016 was performed. Results: In 32/55 patients (58.2%), multi-organ metastases were identified (the most common sites being bone, lungs, and lymph nodes). Of the remaining 23 patients, the liver was the unique metastatic site; thirteen patients had diffuse bilobar hepatic metastases. The remaining ten patients were proposed for surgical treatment; three of them had peritoneal carcinomatosis identified during surgery, and no hepatic metastasectomy was performed. As a result, only seven (12.7%) patients underwent liver metastasectomy. Overall survival was higher in patients who had LM surgery (65 months [Interquartile Range (IQR) 54–120]), in comparison to those diagnosed with diffuse bilobar hepatic metastases (17.5 months [IQR 11–41]), and with those showing concurrent liver and bone metastases (16.5 months [IQR 6–36]) (p = 0.012). In univariable analysis, the latter two groups showed worse overall survival outcomes (Hazard Ratio (HR) = 3.447, 95%CI: 1.218–9.756, p = 0.02 and HR = 3.855, 95% Confidence Interval (CI): 1.475–10.077, p = 0.006, respectively) when compared to patients with LM. Conclusion: In our series, patients submitted to metastasectomy had a median overall survival after diagnosis of LM three times greater than the non-operated patients with isolated LM, or concurrent LM and bone metastases (65 vs. 17.5 and 16.5 months, respectively). As is vastly known for colorectal cancer liver metastasis, resection of breast cancer liver metastasis may reduce tumor burden, and therefore may improve patient outcome.
The Effects of MQL and Dry Environments on Tool Wear, Cutting Temperature, and Power Consumption during End Milling of AISI 1040 Steel
Authors: Salur Emin , Kuntoğlu Mustafa , Aslan Abdullah , Pimenov Danil Yurievich
Published: October 2021

Minimum quantity lubrication (MQL) is a sustainable method that has been efficiently applied to achieve machinability improvements with various materials in recent years, such as hardened steels, superalloys, soft metals, and composites. This study is the first to focus on the performance evaluation of MQL and dry milling environments with AISI 1040 steel. The tool wear, cutting temperature, and power consumption were considered as the quality responses while cutting speed, feed rate and machining environment are taken as input parameters. The effects of the influential factors are analyzed using analysis of variance (ANOVA) and bar charts. Additionally, Taguchi signal-to-noise (S/N) ratios are utilized in order to determine the optimum parameters for the best quality responses. The results show that the MQL system provides better performance compared to dry milling by reducing the tool wear, cutting temperature, and power consumption. According to the ANOVA results, the cutting environment affects the cutting temperature (37%) and power consumption (94%), while cutting speed has importance effects on the tool wear (74%). A lower cutting speed (100 m/min) and feed rate (0.10 mm/rev) should be selected under MQL conditions to ensure minimum tool wear and power consumption; however, a higher feed rate (0.15 mm/rev) needs to be selected along with a low cutting speed and MQL conditions to ensure better temperatures. A comparative evaluation is carried out on the tool wear, cutting temperature, and power consumption under MQL and dry environments. This investigation is expected to contribute to the current literature, highlighting the superiority of sustainable methods in the milling of industrially important materials.
Role of Electrode Rotation on Improvement of Metal Pool Profile in Electroslag Remelting Process
Authors: Huang Xuechi , Duan Yiru , Liu Zhongqiu , Li Baokuan , Wang Fang
Published: October 2021

A comprehensive transient model is developed to study the effect of electrode rotation on the evolution of metal pool profiles and the solidification quality of ESR ingots. Magnetohydrodynamic flow, heat transfer, solidification, and electrode melting are considered simultaneously in the model. The growth of the ESR ingot is predicted using the dynamic layering method. The numerical results show that the productivity reaches a maximum of 15.97% at the rotating speed of 40 rpm without increasing power. With the increasing rotating speed, the maximum temperature of the melt decreases, and the temperature distribution becomes more uniform. Compared with the static one, the pool profiles are flattened by −1.19%, −8.52%, and 12.44% at the rotating speeds of 20, 40, and 60 rpm, respectively. The metal pool profile was improved remarkably, but only at the higher speed (i.e., 60 rpm). The effect of rotating speed on the metal pool profile depends on the competition between the melting rate and slag temperature. Meanwhile, the local solidification time and the secondary dendrite arm spacing are slightly improved at lower rotating speeds but are significantly worse at higher rotating speeds.
Epidemiology of Legionnaires’ Disease in Italy, 2004–2019: A Summary of Available Evidence
Authors: Riccò Matteo , Peruzzi Simona , Ranzieri Silvia , Giuri Pasquale Gianluca
Published: October 2021

Legionnaires’ disease (LD) incidence has been increasing in several European countries since 2011. Currently, Italy is experiencing high notification rates for LD, whose cause still remains scarcely understood. We sought to summarize the available evidence on the epidemiology of LD in Italy (2004–2019), characterizing the risk of LD by region, sex, age group, and settings of the case (i.e., community, healthcare, or travel-associated cases). Environmental factors (e.g., average air temperatures and relative humidity) were also included in a Poisson regression model in order to assess their potential role on the annual incidence of new LD cases. National surveillance data included a total of 23,554 LD cases occurring between 2004 and 2019 (70.4% of them were of male gender, 94.1% were aged 40 years and older), with age-adjusted incidence rates increasing from 1.053 cases per 100,000 in 2004 to 4.559 per 100,000 in 2019. The majority of incident cases came from northern Italy (43.2% from northwestern Italy, 25.6% from northeastern Italy). Of these, 5.9% were healthcare-related, and 21.1% were travel-associated. A case-fatality ratio of 5.2% was calculated for the whole of the assessed timeframe, with a pooled estimate for mortality of 0.122 events per 100,000 population per year. Poisson regression analysis was associated with conflicting results, as any increase in average air temperature resulted in reduced risk for LD cases (Incidence Rate Ratio [IRR] 0.807, 95% Confidence Interval [95% CI] 0.744–0.874), while higher annual income in older individuals was associated with an increased IRR (1.238, 95% CI 1.134–1.351). The relative differences in incidence between Italian regions could not be explained by demographic factors (i.e., age and sex distribution of the population), and also a critical reappraisal of environmental factors failed to substantiate both the varying incidence across the country and the decennial trend we were able to identify.
Study on the Role of the Common House Fly, Musca domestica, in the Spread of ORF Virus (Poxviridae) DNA under Laboratory Conditions
Authors: Raele Donato , Stoffolano John , Vasco Ilaria , Pennuzzi Germana , Nardella La Porta Maria , Cafiero Maria
Published: October 2021

ORF virus (Poxviridae) is the causative agent of contagious ecthyma (soremouth), a disease primarily affecting sheep and goats worldwide, but also humans exposed to disease-ridden animals. Pathogens are shed with scabs, and infection mainly occurs by direct contact. Although the disease is relatively benign and self-limiting, the morbidity rate is high in livestock with subsequent significant financial and economic impact. The aim of the study was to experimentally investigate the potential for the housefly, Musca domestica, to act as a mechanical vector of the virus. Homogenate of crusted scabs from ORFV-positive sheep (Italy, Apulia) were used to infect laboratory-reared flies. Flies walking on viral mixture and flies inoculated on their wings were individually placed in Falcon tubes and the ORFV DNA was searched by PCR on tube walls; flies were fed on the same homogenized crusts and their crop and spots (vomit and feces) molecularly examined for ORF DNA at 2, 4, and 6 h. All of the flies (100%) used in the experiments were able to pick up and transmit the viral genome to contact surfaces; 60% were found ORF virus (DNA)-positive in both spots and crop. These results suggest that M. domestica could play a role as potential mechanical vector and/or reservoir in the epidemiology of the ORF virus infection. Thus, house fly management should be considered in the measures to control the disease in ovine–caprine farms.
Active Surveillance for Highly Pathogenic Avian Influenza Viruses in Wintering Waterbirds in Northeast Italy, 2020–2021
Authors: Gobbo Federica , Fornasiero Diletta , De Marco Maria Alessandra , Zecchin Bianca , Mulatti Paolo , Delogu Mauro , Terregino Calogero
Published: October 2021

The increasing involvement of wild waterfowl in H5 Highly Pathogenic Avian Influenza Virus (HPAIV) circulation continues to pose a threat to animal and public health worldwide. In winter 2020–2021, two field surveillance activities were carried out on a weekly basis, through virological and serological analyses, in 823 hunted and 521 trapped migratory aquatic birds in northeast Italy. Sixty Eurasian teals were recaptured several times, which allowed us to follow the progression of the HPAI H5 infection in naturally infected wild waterfowl. Oropharyngeal, cloacal, and feather swabs (OS, CS and FS) were collected from each duck and tested by real time rRT-PCR Type A influenza. The identified viruses were characterized and pathotyped by sequencing. Several viruses belonging to three different HPAI H5 subtypes were detected: H5N8, H5N5, and H5N1. High prevalence of infection with HPAI H5 clade 2.3.4.4b during November–December 2020 (up to 27.1%) was observed in captured Eurasian teals, while infection rates in hunted dabbling ducks, mainly Eurasian wigeons, showed the highest prevalence of infection in November 2020 (8.9%) and January 2021 (10.2%). All HPAI positive birds were also clinically healthy when recaptured weeks apart. The OS and FS showed the highest detection efficiency of HPAIV. Our results highlight that HPAI passive surveillance should be complemented by a targeted active surveillance to more efficiently detect novel HPAI viruses.
Hybrid Chelator-Based PSMA Radiopharmaceuticals: Translational Approach
Authors: Lahnif Hanane , Grus Tilmann , Pektor Stefanie , Greifenstein Lukas , Schreckenberger Mathias , Rösch Frank
Published: October 2021

(1) Background: Prostate-specific membrane antigen (PSMA) has been extensively studied in the last decade. It became a promising biological target in the diagnosis and therapy of PSMA-expressing cancer diseases. Although there are several radiolabeled PSMA inhibitors available, the search for new compounds with improved pharmacokinetic properties and simplified synthesis is still ongoing. In this study, we developed PSMA ligands with two different hybrid chelators and a modified linker. Both compounds have displayed a promising pharmacokinetic profile. (2) Methods: DATA5m.SA.KuE and AAZTA5.SA.KuE were synthesized. DATA5m.SA.KuE was labeled with gallium-68 and radiochemical yields of various amounts of precursor at different temperatures were determined. Complex stability in phosphate-buffered saline (PBS) and human serum (HS) was examined at 37 °C. Binding affinity and internalization ratio were determined in in vitro assays using PSMA-positive LNCaP cells. Tumor accumulation and biodistribution were evaluated in vivo and ex vivo using an LNCaP Balb/c nude mouse model. All experiments were conducted with PSMA-11 as reference. (3) Results: DATA5m.SA.KuE was synthesized successfully. AAZTA5.SA.KuE was synthesized and labeled according to the literature. Radiolabeling of DATA5m.SA.KuE with gallium-68 was performed in ammonium acetate buffer (1 M, pH 5.5). High radiochemical yields (>98%) were obtained with 5 nmol at 70 °C, 15 nmol at 50 °C, and 60 nmol (50 µg) at room temperature. [68Ga]Ga-DATA5m.SA.KuE was stable in human serum as well as in PBS after 120 min. PSMA binding affinities of AAZTA5.SA.KuE and DATA5m.SA.KuE were in the nanomolar range. PSMA-specific internalization ratio was comparable to PSMA-11. In vivo and ex vivo studies of [177Lu]Lu-AAZTA5.SA.KuE, [44Sc]Sc-AAZTA5.SA.KuE and [68Ga]Ga-DATA5m.SA.KuE displayed specific accumulation in the tumor along with fast clearance and reduced off-target uptake. (4) Conclusions: Both KuE-conjugates showed promising properties especially in vivo allowing for translational theranostic use.
Cell Wall Composition of Hemp Shiv Determined by Physical and Chemical Approaches
Authors: Diakité Maya-Sétan , Lenormand Hélène , Lequart Vincent , Arufe Santiago , Martin Patrick , Leblanc Nathalie
Published: October 2021

The use of agricultural by-products in the building engineering realm has led to an increase in insulation characteristics of biobased materials and a decrease in environmental impact. The understanding of cell wall structure is possible by the study of interactions of chemical compounds, themselves determined by common techniques like Van Soest (VS). In this study, a global method is investigated to characterise the cell wall of hemp shiv. The cell wall molecules were, at first, isolated by fractionation of biomass and then analysed by physical and chemical analysis (Thermal Gravimetric Analysis, Elementary Analysis, Dynamic Sorption Vapor and Infra-Red). This global method is an experimental way to characterise plant cell wall molecules of fractions by Thermal Gravimetric Analysis following by a mathematical method to have a detailed estimation of the cell wall composition and the interactions between plant macromolecules. The analyzed hemp shiv presents proportions of 2.5 ± 0.6% of water, 4.4 ± 0.2% of pectins, 42.6 ± 1.0% (Hemicellulose–Cellulose), 18.4 ± 1.6% (Cellulose–Hemicellulose), 29.0 ± 0.8% (Lignin–Cellulose) and 2.0 ± 0.4% of linked lignin.
The Spasmolytic, Bronchodilator, and Vasodilator Activities of Parmotrema perlatum Are Explained by Anti-Muscarinic and Calcium Antagonistic Mechanisms
Authors: Hussain Musaddique , Bakhsh Hazoor , Syed Shahzada Khurram , Ullah Malik Saad , Alqahtani Ali M. , Alqahtani Taha , Aldahish Afaf A. , Emran Talha Bin , Rehman Kashif Ur , Janbaz Khalid Hussain
Published: October 2021

Parmotremaperlatum is traditionally used in different areas of Pakistan to treat gastrointestinal, respiratory, and vascular diseases. This study evaluates the underlying mechanisms for traditional uses of P. perlatum in diarrhea, asthma, and hypertension. In vitro pharmacological studies were conducted using isolated jejunum, trachea, and aortic preparations, while the cytotoxic study was conducted in mice. Crude extract of P. perlatum(Pp.Cr), comprising appreciable quantities of alkaloids and flavonoids, relaxed spontaneously contracting jejunum preparation, K+ (80 mM)-induced, and carbachol (1 µM)-induced jejunum contractions in a concentration-dependent manner similar to dicyclomine and dantrolene. Pp.Cr showed a rightward parallel shift of concentration-response curves (CRCs) of Cch after a non-parallel shift similarto dicyclomine and shifted CRCs of Ca+2 to rightward much likeverapamil and dantrolene, demonstrating the coexistence of antimuscarinic and Ca+2 antagonistic mechanism. Furthermore, Pp.Cr, dicyclomine, and dantrolene relaxed K+ (80 mM)-induced and Cch (1 µM)-induced tracheal contractions and shifted rightward CRCs of Cch similar to dicyclomine, signifying the dual blockade. Additionally, Pp.Cr also relaxed the K+ (80 mM)-induced and phenylephrine (1 µM)-induced aortic contraction, similarly to verapamil and dantrolene, suggesting Ca+2 channel antagonism. Here, we explored for the first time thespasmolytic and bronchodilator effects of Pp.Crand whether they maybe due to the dual blockade of Ca+2 channels and muscarinic receptors, while the vasodilator effect might be owing to Ca+2 antagonism. Our results provide the pharmacological evidence that P. perlatum could be a new potential therapeutic option to treat gastrointestinal, respiratory, and vascular diseases. Hence, there is a need for further research to explore bioactive constituent of P. perlatum as well as further investigation by suitable experimental models are required to further confirm the importance and usefulness of P. perlatum in diarrhea, asthma, and hypertension treatment.
A Network Analysis on Cloud Gaming: Stadia, GeForce Now and PSNow
Authors: Di Domenico Andrea , Perna Gianluca , Trevisan Martino , Vassio Luca , Giordano Danilo
Published: October 2021

Cloud gaming is a class of services that promises to revolutionize the videogame market. It allows the user to play a videogame with essential equipment while using a remote server for the actual execution. The multimedia content is streamed through the network from the server to the user. Hence, this service requires low latency and a large bandwidth to work properly with low response time and high-definition video. Three of the leading tech companies (Google, Sony, and NVIDIA) entered this market with their products, and others, like Microsoft and Amazon, are also launching their platforms. However, these companies have released little information about their cloud gaming operation and how they utilize the network. In this work, we study cloud gaming services from the network point of view. We collect more than 200 packet traces under different application settings and network conditions from a broadband network to poor mobile network conditions, for 3 cloud gaming services, namely Stadia from Google, GeForce Now from NVIDIA and PS Now from Sony. We analyze the employed protocols and the workload that they impose on the network. We find that GeForce Now and Stadia use the RTP protocol to stream the multimedia content, with the latter relying on the standard WebRTC APIs. Depending on the network and video quality, they result in bandwidth-hungry services consuming up to 45 Mbit/s. PS Now instead uses only undocumented protocols and never exceeds 13 Mbit/s. 4G mobile networks can often sustain these loads, while traditional 3G connections struggle. The systems quickly react to deteriorated network conditions, and packet losses up to 5% do not cause a reduction in resolution.
Influence of the Bioactive Diet Components on the Gene Expression Regulation
Authors: Mierziak Justyna , Kostyn Kamil , Boba Aleksandra , Czemplik Magdalena , Kulma Anna , Wojtasik Wioleta
Published: October 2021

Diet bioactive components, in the concept of nutrigenetics and nutrigenomics, consist of food constituents, which can transfer information from the external environment and influence gene expression in the cell and thus the function of the whole organism. It is crucial to regard food not only as the source of energy and basic nutriments, crucial for living and organism development, but also as the factor influencing health/disease, biochemical mechanisms, and activation of biochemical pathways. Bioactive components of the diet regulate gene expression through changes in the chromatin structure (including DNA methylation and histone modification), non-coding RNA, activation of transcription factors by signalling cascades, or direct ligand binding to the nuclear receptors. Analysis of interactions between diet components and human genome structure and gene activity is a modern approach that will help to better understand these relations and will allow designing dietary guidances, which can help maintain good health.
Are Nutritional Patterns among Polish Hashimoto Thyroiditis Patients Differentiated Internally and Related to Ailments and Other Diseases?
Authors: Ihnatowicz Paulina , Wątor Paweł , Gębski Jerzy , Frąckiewicz Joanna , Drywień Małgorzata Ewa
Published: October 2021

There is not any diet recommended for Hashimoto’s disease, despite that those patients are often undernourished. Because of the high heterogeneity of Hashimoto’s patients, insight into dietary patterns might shed some light on the patient-tailored dietary approach, thus improving their treatment and helping to identify patients with the highest probability of particular nutritional deficiencies. The aim of this study was to identify Hashimoto’s patients’ dietary patterns and their characterization based on both socio-demographic variables and dietary self-assessment. We collected data online from patients with Hashimoto’s disease. The questionnaire formula used in the study was developed based on a validated food frequency questionnaire KomPAN®. K-means pattern analyses were used to characterize patients into patterns based on the frequency of particular types of foods consumption and socio-demographic factors. Four patterns were identified. We labeled them as ‘Convenient’, ‘Non-meat’, ‘Pro-healthy’, and ‘Carnivores’ with participants proportions at approximately one-fourth per each pattern. The patients were mainly of the female gender (94.08%), with a female: male ratio of 15.9. Hashimoto’s patients differed in their food product choices, food choice motives, dieting experience, nutritional knowledge, smoking habits, food allergies and intolerances, and lipid disorders, and thus represent different eating patterns. However, these patterns were not determined by comorbidities or the majority of ailments.
Chrysanthemum indicum Prevents Hydrogen Peroxide-Induced Neurotoxicity by Activating the TrkB/Akt Signaling Pathway in Hippocampal Neuronal Cells
Authors: Jeong Yun Hee , Kim Tae In , Oh You-Chang , Ma Jin Yeul
Published: October 2021

Oxidative stress-mediated neuronal damage is associated with the pathogenesis and development of neurodegenerative diseases. Chrysanthemum indicum has antioxidant properties. However, the neuroprotective effects and the cellular mechanism of C. indicum ethanol extract (CIE) against oxidative damage in hippocampal neuronal cells have not been clearly elucidated. Therefore, this study investigated whether CIE has protective effects against hydrogen peroxide (H2O2)-induced oxidative toxicity in HT22 cells. CIE pretreatment significantly improved neuronal cell viability. Moreover, the formation of intracellular reactive oxygen species and apoptotic bodies, and mitochondrial depolarization were significantly reduced in HT22 cells with H2O2-induced oxidative toxicity. Furthermore, CIE increased the phosphorylation of tropomyosin-related kinase receptor B (TrkB), protein kinase B (Akt), cAMP response element-binding protein, the expression of brain-derived neurotrophic factor, antioxidant enzymes, and the nuclear translocation of nuclear factor erythroid 2-related factor 2 by activating the TrkB/Akt signaling pathway. In contrast, the addition of K252a, a TrkB inhibitor, or MK-2206, an Akt-selective inhibitor, reduced the neuroprotective and antioxidant effects of CIE. Taken together; CIE exhibits neuroprotective and antioxidant effects against oxidative damage. Therefore, it can be a potential agent for treating oxidative stress-related neurodegenerative diseases.
Extraintestinal Pathogenic Escherichia coli: Virulence Factors and Antibiotic Resistance
Authors: Sora Valerio M. , Meroni Gabriele , Martino Piera A. , Soggiu Alessio , Bonizzi Luigi , Zecconi Alfonso
Published: October 2021

The One Health approach emphasizes the importance of antimicrobial resistance (AMR) as a major concern both in public health and in food animal production systems. As a general classification, E. coli can be distinguished based on the ability to cause infection of the gastrointestinal system (IPEC) or outside of it (ExPEC). Among the different pathogens, E. coli are becoming of great importance, and it has been suggested that ExPEC may harbor resistance genes that may be transferred to pathogenic or opportunistic bacteria. ExPEC strains are versatile bacteria that can cause urinary tract, bloodstream, prostate, and other infections at non-intestinal sites. In this context of rapidly increasing multidrug-resistance worldwide and a diminishingly effective antimicrobial arsenal to tackle resistant strains. ExPEC infections are now a serious public health threat worldwide. However, the clinical and economic impact of these infections and their optimal management are challenging, and consequently, there is an increasing awareness of the importance of ExPECs amongst healthcare professionals and the general public alike. This review aims to describe pathotype characteristics of ExPEC to increase our knowledge of these bacteria and, consequently, to increase our chances to control them and reduce the risk for AMR, following a One Health approach.
Virucidal Activity of Plant Extracts against African Swine Fever Virus
Authors: Juszkiewicz Małgorzata , Walczak Marek , Woźniakowski Grzegorz , Szczotka-Bochniarz Anna
Published: October 2021

African swine fever is one of the most dangerous and fatal swine diseases, described for the first time roughly a hundred years ago. Even now, there is neither a commercially approved vaccine nor treatment available. The only way to hinder further spread of the disease is by culling the affected herds and applying prevention based mainly on proper biosecurity. Due to growing awareness of the potential ASF threat among pig producers, disinfection processes are considered as one of the most important preventive measures. Currently, a variety of chemical compounds are applied for the disinfection of pig farms. Meanwhile, these chemicals may pose a potential risk, due to their toxic, irritant or corrosive effect. The aim of this study was to determine whether any plant-based natural compounds may show a virucidal effect against ASFV, and simultaneously be depleted of some of the side-effects typical for chemical compounds. Ideally, natural virucidal compounds should be safe for both humans and animals, biodegradable, easily available and inexpensive. Fourteen plant extracts were selected and screened for their virucidal effect against ASFV, using the suspension test inspired by the PN-EN 14675:2015 European Standard procedure. The results of our study showed that most of the tested plant extracts were ineffective against ASFV. Some extracts suspended in a hydroglycolic medium exhibited high virus titre reduction, but it was confirmed that the effect resulted from medium composition. However, a 1.05% peppermint extract showed high effectiveness against ASFV, reducing the virus titre by ≥4 log10, thus demonstrating that natural compounds used as virucidal agents could potentially be used in disinfection procedures, being both effective and harmless to humans and animals.
Diode Laser Lithotription Technique Based on Optothermal Converter
Authors: Streltsova Olga , Grebenkin Evgeny , Bityurin Nikita , Bredikhin Vladimir , Elagin Vadim , Vlasov Vasily , Kamensky Vladislav
Published: October 2021

Purpose: evaluation of the efficiency of the “hot spot” method for the fragmentation of urinary stones. Materials and methods: A retrospective analysis of clinical records of 1666 patients with urolithiasis who underwent percutaneous nephrolithoextraction/tripsy and contact ureterolithotripsy/extraction in the period from 2014 to 2017 at the urology clinic was performed to assess the incidence of postoperative infectious and inflammatory complications. The research objects were postoperative urinary stones (n-78). The X-ray density and linear dimensions of the stones were determined. Stone fragmentation was performed with a continuous-wave diode laser operating at wavelengths of 0.81 μm, 0.97 μm, and 1.47 μm. An absorbing coating of micro-size graphite powder was applied on the working tip of the optical fiber. In vitro fragmentation was carried out in liquid. Results: A group of patients (224/1666) (13.4 ± 0.86%) was identified, who developed infectious and inflammatory complications after: percutaneous nephrolithotripsy, 123/361 (34.1 ± 2.5%) cases; percutaneous nephrolithoextraction, 59/240 (24.6 ± 2.78%); contact ureterolithotripsy, 23/294 (7.8 ± 1.57%); and ureterolithoextraction, 19/771 (2.5 ± 0.56%). In liquid, the “hot spot” technique made it possible to fragment stones with an X-ray density of up to 1000 HU at a laser wavelength of 0.81 µm, up to 1400 HU at 0.97 µm, and up to 1400 HU at 1.47 µm.
Direct and Clean Loading of Nanoparticles into Optical Traps at Millibar Pressures
Authors: Nikkhou Maryam , Hu Yanhui , Sabin James A. , Millen James
Published: October 2021

Nanoparticles levitated by optical fields under vacuum conditions have applications in quantum science, the study of nanothermodynamics and precision sensing. The existing techniques for loading optical traps require ambient conditions and often involve dispersion in liquids, which can contaminate delicate optics and lead to enhanced optical absorption and heating. Here, we present a clean, dry and generic mechanism for directly loading optical traps at pressures down to 1 mbar, exploiting Laser Induced Acoustic Desorption and allowing for the rapid and efficient trapping of nanoparticles.
Accurate 3D Shape Reconstruction from Single Structured-Light Image via Fringe-to-Fringe Network
Authors: Nguyen Hieu , Wang Zhaoyang
Published: October 2021

Accurate three-dimensional (3D) shape reconstruction of objects from a single image is a challenging task, yet it is highly demanded by numerous applications. This paper presents a novel 3D shape reconstruction technique integrating a high-accuracy structured-light method with a deep neural network learning scheme. The proposed approach employs a convolutional neural network (CNN) to transform a color structured-light fringe image into multiple triple-frequency phase-shifted grayscale fringe images, from which the 3D shape can be accurately reconstructed. The robustness of the proposed technique is verified, and it can be a promising 3D imaging tool in future scientific and industrial applications.
Change in H+ Transport across Thylakoid Membrane as Potential Mechanism of 14.3 Hz Magnetic Field Impact on Photosynthetic Light Reactions in Seedlings of Wheat (Triticum aestivum L.)
Authors: Sukhova Ekaterina , Gromova Ekaterina , Yudina Lyubov , Kior Anastasiia , Vetrova Yana , Ilin Nikolay , Mareev Evgeny , Vodeneev Vladimir , Sukhov Vladimir
Published: October 2021

Natural and artificial extremely low-frequency magnetic fields (ELFMFs) are important factors influencing physiological processes in living organisms including terrestrial plants. Earlier, it was experimentally shown that short-term and long-term treatments by ELFMFs with Schumann resonance frequencies (7.8, 14.3, and 20.8 Hz) influenced parameters of photosynthetic light reactions in wheat leaves. The current work is devoted to an analysis of potential ways of this ELFMF influence on the light reactions. Only a short-term wheat treatment by 14.3 Hz ELFMF was used in the analysis. First, it was experimentally shown that ELFMF-induced changes (an increase in the effective quantum yield of photosystem II, a decrease in the non-photochemical quenching of chlorophyll fluorescence, a decrease in time of changes in these parameters, etc.) were observed under the action of ELFMF with widely ranging magnitudes (from 3 to 180 µT). In contrast, the potential quantum yield of photosystem II and time of relaxation of the energy-dependent component of the non-photochemical quenching were not significantly influenced by ELFMF. Second, it was shown that the ELFMF treatment decreased the proton gradient across the thylakoid membrane. In contrast, the H+ conductivity increased under this treatment. Third, an analysis of the simplest mathematical model of an H+ transport across the thylakoid membrane, which was developed in this work, showed that changes in H+ fluxes related to activities of the photosynthetic electron transport chain and the H+-ATP synthase were not likely a mechanism of the ELFMF influence. In contrast, changes induced by an increase in an additional H+ flux (probably, through the proton leakage and/or through the H+/Ca2+ antiporter activity in the thylakoid membrane) were in good accordance with experimental results. Thus, we hypothesized that this increase is the mechanism of the 14.3 Hz ELFMF influence (and, maybe, influences of other low frequencies) on photosynthetic light reactions in wheat.
Comparative Phytochemical Profile and Biological Activity of Four Major Medicinal Halophytes from Qassim Flora
Authors: Mohammed Hamdoon A. , Ali Hussein M. , Qureshi Kamal A. , Alsharidah Mansour , Kandil Yasser I. , Said Rana , Mohammed Salman A. A. , Al-Omar Mohsen S. , Rugaie Osamah Al , Abdellatif Ahmed A. H. , Abd-Elmoniem Essam , Abbas Manal M. , Mohany Khalid M. , Khan Riaz A.
Published: October 2021

Four halophytic plants, Lycium shawii, Anabasis articulata, Rumex vesicarius, and Zilla spinosa, growing in the central Qassim area, Saudi Arabia, were phytochemically and biologically investigated. Their hydroalcoholic extracts’ UPLC-ESIQ-TOF analyses demonstrated the presence of 44 compounds of phenolic acids, flavonoids, saponins, carbohydrates, and fatty acids chemical classes. Among all the plants’ extracts, L. shawii showed the highest quantities of total phenolics, and flavonoids contents (52.72 and 13.01 mg/gm of the gallic acid and quercetin equivalents, respectively), along with the antioxidant activity in the TAA (total antioxidant activity), FRAP (ferric reducing antioxidant power), and DPPH-SA (2,2-diphenyl-1-picryl-hydrazyl-scavenging activity) assays with 25.6, 56.68, and 19.76 mg/gm, respectively, as Trolox equivalents. The hydroalcoholic extract of the L. shawii also demonstrated the best chelating activity at 21.84 mg/gm EDTA equivalents. Among all the four halophytes, the hydroalcoholic extract of L. shawii exhibited the highest antiproliferative activity against MCF7 and K562 cell lines with IC50 values at 194.5 µg/mL and 464.9 µg/mL, respectively. The hydroalcoholic extract of A. articulata demonstrated better cytotoxic activity amongst all the tested plants’ extracts against the human pancreatic cancer cell lines (PANC1) with an IC50 value of 998.5 µg/mL. The L. shawii induced apoptosis in the MCF7 cell lines, and the percentage of the necrotic cells changed to 28.1% and 36.5% for the IC50 and double-IC50 values at 22.9% compared with the untreated groups. The hydroalcoholic extract of L. shawii showed substantial antibacterial activity against Bacillus cereus ATCC 10876 with a MIC value of 12.5 mg/mL. By contrast, the A. articulata and Z. spinosa exhibited antifungal activities against Aspergillus niger ATCC 6275 with MIC values at 12.5 and 50 mg/mL, respectively. These findings suggested that the L. shawii is a potential halophyte with remarkable biological properties, attributed to its contents of phenolics and flavonoid classes of compounds in its extract.
WRKY Transcription Factor Response to High-Temperature Stress
Authors: Cheng Zhuoya , Luan Yuting , Meng Jiasong , Sun Jing , Tao Jun , Zhao Daqiu
Published: October 2021

Plant growth and development are closely related to the environment, and high-temperature stress is an important environmental factor that affects these processes. WRKY transcription factors (TFs) play important roles in plant responses to high-temperature stress. WRKY TFs can bind to the W-box cis-acting elements of target gene promoters, thereby regulating the expression of multiple types of target genes and participating in multiple signaling pathways in plants. A number of studies have shown the important biological functions and working mechanisms of WRKY TFs in plant responses to high temperature. However, there are few reviews that summarize the research progress on this topic. To fully understand the role of WRKY TFs in the response to high temperature, this paper reviews the structure and regulatory mechanism of WRKY TFs, as well as the related signaling pathways that regulate plant growth under high-temperature stress, which have been described in recent years, and this paper provides references for the further exploration of the molecular mechanisms underlying plant tolerance to high temperature.