bluuebunny/crossref_metadata_2025 huggingface dataset:
Motor unit contributions to activation reduction and torque steadiness following active lengthening: a study of residual torque enhancement
Authors: Jakobi Jennifer M. , Kuzyk Samantha L. , McNeil Chris J. , Dalton Brian H. , Power Geoffrey A.
Published: April 2020

Our findings indicate that lower electromyographic activity during the torque-enhanced condition following active lengthening compared with a purely isometric contraction arises from fewer active motor units and a lower discharge rate of those that are active. We used an acute condition of increased torque capacity to induce a decrease in net output of the motor neuron pool during a submaximal task to demonstrate, in humans, the impact of motor unit activity on torque steadiness.
Um oásis num sistema poluído
Authors: Bezerra Amilcar Almeida , Ferreira Daniela Maria
Published: April 2020

Neste artigo, utilizamos depoimentos de agentes pioneiros na criação de festivais e lojas especializadas em rock em Pernambuco para reconstruir os fragmentos de uma rede de sociabilidade em torno desse gênero musical nos anos 1970. Todos fazem referência a experiências marcantes vivenciadas na casa de Humberto Brito, distribuidor informal de lançamentos importados raros na cidade. Utilizando a noção de “mediação cosmopolita”, elaborada a partir das contribuições de Latour (2012), Silverstone (2002) e Robbins (1992), tentamos compreender de que maneira se desenvolve a constituição de determinado gosto e de um modo específico de se relacionar com a música naquele contexto.
Receitas para o amor, por Aracy e João Guimarães Rosa
Authors: Biazioli Tânia
Published: April 2020

É este um estudo sobre os prazeres da mesa na vida e obra de Guimarães Rosa. Serão aqui analisados tanto os cadernos de receitas culinárias de sua esposa, Aracy, quanto os romances “Dão-Lalalão” e “Buriti”, de João Guimarães Rosa. Nossa hipótese era que as receitas poderiam trazer à tona a cozinha mineira. Descobrimos que a vida doméstica era o espaço onde se cultivava a culinária cosmopolita alemã, enquanto a literatura rosiana era o espaço onde se preservava a culinária regionalista mineira. Descobrimos também um convite das receitas para o amor, que aparece tanto nos nomes de doces, quanto nos romances de Noites do sertão. Afinal, a psicanálise mostra que o ato de comer busca a satisfação da fome e do amor.
El fondo Aracy Abreu Amaral y la historiografía del arte de América Latina
Authors: Santos Renata Ribeiro dos
Published: April 2020

The present text presents an initial review of the research carried out at the Aracy Abreu Amaral Fund, guarded by the Instituto de Estudos Brasileiros of the University of São Paulo. The fundamental objective of the study is to establish the relations and intellectual exchanges between the different agents who, together with Amaral, were decisive in the delimitation and diffusion of Latin American art between the 1970s and 1990s.
Grande sertão: veredas, um inventário da avifauna
Authors: Dolberth Willian , Eggensperger Klaus
Published: April 2020

O presente trabalho se propõe a inventariar as aves citadas no romance Grande sertão: veredas, classificando seus usos e traçando um panorama do processo criativo que as levou até as páginas do livro. Nosso objetivo é desvelar a natureza da representação da avifauna na obra, uma alternativa de leitura ecologicamente consciente que se opõe à perspectiva antropocêntrica que retrata os elementos da natureza de forma opaca e pitoresca. Acreditamos que uma abordagem ecocrítica nos permite reconhecer novos aspectos do complexo processo de criação rosiano, um amálgama de ciência natural e cultura popular originando uma mimese da natureza que fecunda diversos outros motivos dentro da obra.
An update on pityrosporum folliculitis in Singapore from a single tertiary care dermatological centre
Authors: Yong AM , Tan SY , Tan LC
Published: April 2020

INTRODUCTION Pityrosporum folliculitis (PF) is a common skin condition that can be easily misdiagnosed, especially by non-dermatologists. While the initial diagnosis is often made clinically, skin microscopy may help to confirm the same. However, there is scant literature regarding the clinical epidemiology of PF. To our knowledge, in Singapore, only one prior epidemiological study was performed in 1987. Through the present study, we aimed to provide an update regarding the epidemiology, diagnosis and treatment of patients with PF in Singapore. METHODS We performed a retrospective review of patients with clinical presentations compatible with PF who presented to the dermatology clinic at the National University Hospital, Singapore, between 1 January 2011 and 31 December 2015. The medical records of patients identified as having clinical presentations that resembled PF were reviewed via written and electronic databases. Information regarding the demographics and clinical presentation of the patients was collected. RESULTS Of the 375 patients identified, 214 (57.1%) were confirmed as having PF based on Gram-stain microscopy. Most (35.0%) of these 214 patients were aged between 21 and 30 years, with a male-to-female ratio of 3:1. The lesions predominantly occurred on the trunk and the back. The majority of patients presented with symptoms that lasted more than one month. 128 (59.8%) patients received oral antifungal treatment, whereas 82 (38.3%) patients were treated with topical antifungal treatment alone. CONCLUSION A typical Singapore patient with PF is a young man aged 21–30 years, with erythematous follicular papules or pustules over the trunk and the back.
Chapter of Gastroenterologists professional guidance for management of patients with liver disease in Singapore during the COVID-19 pandemic
Authors: Chang PE , Wong YJ , Yang WL , Lim KBL , Tan PS , Ho GH , Yip BCH , Li JW , Chong CH , Ong D , Chua TS , Vu C , Gwee KA , Ang TL , Tan CK
Published: April 2020

In this paper, we aim to provide professional guidance to clinicians who are managing patients with chronic liver disease during the current coronavirus disease 2019 (COVID-19) pandemic in Singapore. We reviewed and summarised the available relevant published data on liver disease in COVID-19 and the advisory statements that were issued by major professional bodies, such as the American Association for the Study of Liver Diseases and European Association for the Study of the Liver, contextualising the recommendations to our local situation.
The Understanding and Uses of the “Day Court Diagram” in Qin-Han Hemerology and Calendrical Astrology
Authors: Kalinowski Marc
Published: April 2020

Archaeological discoveries related to hemerology and calendrical astrology have drawn attention to the existence of a square geometric pattern consisting in its simplest form of a central cross and four right angles in the corners opening to the exterior. In this paper I examine the most common terms used in modern research to name the diagram. I then rank the existing diagrams based on the specific contexts for which they were designed, along with a presentation of the technical features of each diagram. Finally, I show how the various forms taken by these diagrams may lead to a better understanding of their meaning and functions and provide preliminary answers to questions such as the purpose of inserting a diagram in a technical text, the advantage of a diagram compared with a list or a table, and more generally, the relationship between text and image in the excavated manuscripts.
Scientific Communities Striving for a Common Cause: Innovations in Carbon Cycle Science
Authors: Whelan Mary E. , Anderegg Leander D. L. , Badgley Grayson , Campbell J. Elliott , Commane Roisin , Frankenberg Christian , Hilton Timothy W. , Kuai Le , Parazoo Nicholas , Shiga Yoichi , Wang Yuting , Worden John
Published: April 2020

Where does the carbon released by burning fossil fuels go? Currently, ocean and land systems remove about half of the CO2 emitted by human activities; the remainder stays in the atmosphere. These removal processes are sensitive to feedbacks in the energy, carbon, and water cycles that will change in the future. Observing how much carbon is taken up on land through photosynthesis is complicated because carbon is simultaneously respired by plants, animals, and microbes. Global observations from satellites and air samples suggest that natural ecosystems take up about as much CO2 as they emit. To match the data, our land models generate imaginary Earths where carbon uptake and respiration are roughly balanced, but the absolute quantities of carbon being exchanged vary widely. Getting the magnitude of the flux is essential to make sure our models are capturing the right pattern for the right reasons. Combining two cutting-edge tools, carbonyl sulfide (OCS) and solar-induced fluorescence (SIF), will help develop an independent answer of how much carbon is being taken up by global ecosystems. Photosynthesis requires CO2, light, and water. OCS provides a spatially and temporally integrated picture of the “front door” of photosynthesis, proportional to CO2 uptake and water loss through plant stomata. SIF provides a high-resolution snapshot of the “side door,” scaling with the light captured by leaves. These two independent pieces of information help us understand plant water and carbon exchange. A coordinated effort to generate SIF and OCS data through satellite, airborne, and ground observations will improve our process-based models to predict how these cycles will change in the future.
Remote Tropical Western Indian Ocean Forcing on Changes in June Precipitation in South China and the Indochina Peninsula
Authors: Leung Marco Y.-T. , Zhou Wen , Wang Dongxiao , Chan P. W. , Lee S. M. , Tong H. W.
Published: April 2020

In this study, remote influence originating from the tropical western Indian Ocean on June precipitation in South China and the Indochina Peninsula is documented. Based on numerical simulation and statistical analysis, it is noted that the warm anomaly in the tropical western Indian Ocean can induce a weaker-than-normal Walker circulation across the tropical Indian Ocean and western Pacific Ocean. This further leads to a northeast–southwest-oriented western North Pacific subtropical high and a weaker-than-normal monsoon trough in the South China Sea. In addition, the weak monsoon trough is concurrent with an anomalous rising motion in South China and a sinking motion in the Indochina Peninsula. This enhances precipitation in South China and suppresses precipitation in the Indochina Peninsula on an interannual time scale. On the other hand, the warming trend in the tropical western Indian Ocean also supports the long-term trends of precipitation in the two regions.
Challenges of Measuring Abyssal Temperature and Salinity at the Kuroshio Extension Observatory
Authors: Anderson Nathan D. , Donohue Kathleen A. , Honda Makio C. , Cronin Meghan F. , Zhang Dongxiao
Published: April 2020

The deep ocean is severely undersampled. Whereas shipboard measurements provide irregular spatial and temporal records, moored records establish deep ocean high-resolution time series, but only at limited locations. Here, highlights and challenges of measuring abyssal temperature and salinity on the Kuroshio Extension Observatory (KEO) mooring (32.3°N, 144.6°E) from 2013 to 2019 are described. Using alternating SeaBird 37-SMP instruments on annual deployments, an apparent fresh drift of 0.03–0.06 psu was observed, with each newly deployed sensor returning to historical norms near 34.685 psu. Recurrent salinity discontinuities were pronounced between the termination of each deployment and the initiation of the next, yet consistent pre- and postdeployment calibrations suggested the freshening was “real.” Because abyssal salinities do not vary by 0.03–0.06 psu between deployment locations, the contradictory salinities during mooring overlap pointed toward a sensor issue that self-corrects prior to postcalibration. A persistent nepheloid layer, unique to KEO and characterized by murky, sediment-filled water, is likely responsible for sediment accretion in the conductivity cell. As sediment (or biofouling) increasingly clogs the instrument, salinity drifts toward a fresh bias. During ascent, the cell is flushed, clearing the clogged instrument. In contrast to salinity, deep ocean temperatures appear to increase from 2013 to 2017 by 0.0059°C, whereas a comparison with historical deep temperature measurements does not support a secular temperature increase in the region. It is suggested that decadal or interannual variability associated with the Kuroshio Extension may have an imprint on deep temperatures. Recommendations are discussed for future abyssal temperature and salinity measurements.
Recommendations for Developing Useful and Usable Convection-Allowing Model Ensemble Information for NWS Forecasters
Authors: Demuth Julie L. , Morss Rebecca E. , Jankov Isidora , Alcott Trevor I. , Alexander Curtis R. , Nietfeld Daniel , Jensen Tara L. , Novak David R. , Benjamin Stanley G.
Published: April 2020

U.S. National Weather Service (NWS) forecasters assess and communicate hazardous weather risks, including the likelihood of a threat and its impacts. Convection-allowing model (CAM) ensembles offer potential to aid forecasting by depicting atmospheric outcomes, including associated uncertainties, at the refined space and time scales at which hazardous weather often occurs. Little is known, however, about what CAM ensemble information is needed to inform forecasting decisions. To address this knowledge gap, participant observations and semistructured interviews were conducted with NWS forecasters from national centers and local weather forecast offices. Data were collected about forecasters’ roles and their forecasting processes, uses of model guidance and verification information, interpretations of prototype CAM ensemble products, and needs for information from CAM ensembles. Results revealed forecasters’ needs for specific types of CAM ensemble guidance, including a product that combines deterministic and probabilistic output from the ensemble as well as a product that provides map-based guidance about timing of hazardous weather threats. Forecasters also expressed a general need for guidance to help them provide impact-based decision support services. Finally, forecasters conveyed needs for objective model verification information to augment their subjective assessments and for training about using CAM ensemble guidance for operational forecasting. The research was conducted as part of an interdisciplinary research effort that integrated elicitation of forecasters’ CAM ensemble needs with model development efforts, with the aim of illustrating a robust approach for creating information for forecasters that is truly useful and usable.
VALIDA: A Validation Therapy-Training Program for Staff of a Residential Care Facility
Authors: Oliveira Mónica P. , Sousa Liliana X.
Published: April 2020

Behavioral and psychological symptoms of dementia are a major reason for institutionalization, and high demanding for staff in Residential Care Facilities. This article reports the design, implementation, and evaluation of a Validation Therapy pilot-training program (VALIDA) for staff members of a residential care facility in Aveiro, Portugal. This pilot study involved 22 members of the staff, including qualified professionals and nonqualified direct care workers, who were involved in the design. The pilot-training program comprises two components: training sessions in group and individual monitoring between those sessions. The evaluation indicates that the structure of the pilot-program was well accepted, and participants reported positive impacts in professional (better skilled to deal with residents with dementia) and personal (better trained to deal with elderly relatives) lives. This pilot-training program shows potential to be used in residential care facilities and needs to be replicated.
Can tranexamic acid reduce the blood transfusion rate in patients undergoing percutaneous nephrolithotomy? A systematic review and meta-analysis
Authors: Wang Zhenghao , He Xiao , Bai Yunjin , Wang Jia
Published: April 2020

Objective

A systematic review and meta-analysis was conducted to explore the efficacy of tranexamic acid (TXA) in reducing transfusion events in patients undergoing percutaneous nephrolithotomy (PCNL).

Methods

PubMed, Web of Science, Embase, EBSCO, and Cochrane library databases from January 1980 to October 2019 were searched for randomized controlled trials (RCTs) that assessed TXA efficacy in reducing transfusion events during PCNL. Intervention treatments include using TXA compared with placebo (or no intervention) for patients who underwent PCNL. The search strategy and study selection process were managed in accordance with the PRISMA statement.

Results

Six RCTs are included in the meta-analysis. Overall, TXA intervention groups showed a significant reduction in blood transfusion events (RR = 0.34; 95% confidence interval [CI] = 0.19 to 0.62), hemoglobin decrease (MD = −0.80; 95% CI = −1.32 to −0.28), operative time (MD = −12.62; 95% CI = −15.62 to −9.61), and length of hospital stay (MD = −0.73; 95% CI = −1.36 to −0.10) compared with control groups after PCNL. However, TXA had no substantial impact on the rate of stone clearance (RR = 1.10; 95% CI = 1.00 to 1.21).

Conclusions

TXA can effectively reduce the transfusion rate and blood loss during PCNL.
Beyond Cyberbullying: Investigating When and How Cybervictimization Predicts Suicidal Ideation
Authors: Quintana-Orts Cirenia , Rey Lourdes , Neto Félix
Published: April 2020

Cybervictimization is a public health concern in adolescence. Victims of cyberbullying may present with important short- and long-term sequelae. Specifically, past research has demonstrated positive associations between being cybervictimized and risk of suicide among adolescents. Nonetheless, the underlying mechanisms that may mediate or moderate this relationship remain unclear. The present research examined perceived stress as a mediator and forgiveness as a moderator to explore the relationship between cybervictimization and suicidal ideation. Specifically, vengeance, avoidance, and benevolence motivations, as a means of measuring forgiveness experienced, were analyzed as moderators in the relations between cybervictimization and suicidal ideation. The sample included 1,821 adolescents (954 girls) who ranged in age from 12 to 17 years from nine centers. Adolescents’ self-reported perceived stress levels, cybervictimization, and forgiveness were collected. A mediation and a moderated mediation analyses were used to explore the aim of the present study. Results suggest that, after controlling for sex and age, perceived stress partially mediates the association between cybervictimization and suicidal ideation. Furthermore, the mediation effects of perceived stress were independently moderated by vengeance and avoidance. Thus, revenge and avoidance might intensify the adverse impact of perceived stress on suicidal ideation. These results highlight how and when cybervictimization is related to suicidal ideation. We discuss the results in relation to previous research and consider their practical implications. These findings suggest that adolescents’ perceived stress, together with vengeance and avoidance motivations, may be key targets for prevention and intervention programs dealing with cybervictimization. This highlights the need to implement treatment and prevention programs focused on decreasing such motivations.
Market Potential and Fiscal Incentives Influence Firms’ Location Decisions: Evidence From U.S. Counties
Authors: Meurers Martin , Moenius Johannes
Published: April 2020

How important are market potential and fiscal incentives for firms’ location decisions? We estimate the influence of subsidies and tax breaks on the decisions of firms to relocate or to remain in a certain U.S. county using a structural economic geography model developed in Meurers and Moenius (2018). In a panel data set from 1990 to 2016 for almost 3,000 U.S. counties, the authors find a strong and robust impact of economic geography on firms’ location decisions: The closer a county is to market demand and to the supply of inputs, the more firms locate there. As the model predicts, public investment attracts firms while the local tax burden disincentivizes economic activity, although to a lesser extent. Furthermore, in counties that are closer to economic centers, firms respond less to public investment and tax changes than firms in counties far away from centers. These data, therefore, confirm the predictions of the model regarding the potential effectiveness of regional development policies, in particular for investment tax credits, job creation, and training.
Debt and crisis: Socio-economic critique of neoliberal transformation in Poland
Authors: Pluciński Przemysław
Published: April 2020

The article offers an analysis of the processes of neoliberal transformation, or the transition from ‘real socialism’ to ‘real capitalism’, which took place roughly three decades ago in Central-Eastern Europe, with particular consideration given to Poland. The key to a sociological understanding of capitalist modernisation is the combination of two perspectives present in social sciences: analysis in terms of shock therapy and the prospect of debt. Referring to the concepts of the ‘rent theory of ownership’, the role of foreign debt, creditor–debtor relations and the resulting crisis are submitted to analysis as the key factors of modernisation. Finally, the social, political and cultural consequences of the neoliberal transformation are also considered. These are argued to be growing right-wing populist and authoritarian tendencies.
Strengthening the Social Information Processing Skills of Third Graders in Rural China
Authors: Guo Shenyang , Peng Jin , Fraser Mark W. , Sun Xiaodong , Wu Fan , Day Steven H.
Published: April 2020

Objective:

The study aimed to describe findings from a matched clusters trial of a school-based intervention Let’s Be Friends designed to promote social competence and prevent maladaptive behavior by strengthening the social information processing (SIP) skills of third-grade children in rural China.

Method:

Using a blocked cluster design with random sampling, 13 treatment schools ( n = 355) of a total of 67 schools in the study site were randomly selected, and 14 schools ( n = 341) formed the control condition. All third graders from the treatment schools received 14 sessions of intervention.

Results:

The adapted program appears to have strengthened children’s SIP skills, reduced aggressive behavior, and promoted cognitive concentration. The study does not confirm that children growing up in single-child families fare worse than children living with siblings.

Conclusion:

The program has the potential to strengthen the SIP skills of children in rural China.
Association of Frailty With Antiplatelet Response Among Elderly Chinese Patients With Coronary Artery Disease Undergoing Percutaneous Coronary Intervention
Authors: Liu Yang , Liu Shaoyan , Wang Keyu , Liu Hongbin
Published: April 2020

Frailty has been implicated as a prognostic factor for ischemic cardiovascular diseases. However, the effects of frailty on platelet responses to aspirin and clopidogrel remain under investigation. In this study, we enrolled consecutive elderly patients with coronary artery disease (CAD) who were treated by percutaneous coronary intervention (PCI) to evaluate this association. A total of 264 patients (aged 70-95 years) were included. Patients were divided into 2 groups: a nonfrail (nFR) group and a frail (FR) group according to the Clinical Frailty Scale. Platelet reactivity was assessed with a light transmittance aggregometry method, and arachidonic acid and adenosine diphosphate induced maximum platelet aggregation (AA-MPA/ADP-MPA) were calculated to evaluate the platelet response to aspirin and clopidogrel. The results showed that the AA-MPA and ADP-MPA of the FR group were significantly higher than those in the nFR group (17.49 ± 6.65 vs 15.19 ± 6.33, P < .01; 56.13 ± 10.14 vs 45.45 ± 11.59, P < .01). High on-aspirin platelet response (HAPR) and high on-clopidogrel platelet response (HCPR) were significantly more common in the FR group than in the nFR group (24.67% vs 13.16%, P = .028, 37.33% vs 15.79%, P < .01). According to multivariable regression analyses, frailty was found to be independently associated with AA-MPA (βcoefficient = 1.883, P = .042) and ADP-MPA (βcoefficient = 9.287, P < .001), and it was an independent predictor of HAPR (odds ratio [OR]: 2.696, P < .01) and HCPR (OR: 2.543, P < .01). It was concluded that among elderly patients with CAD undergoing PCI, frailty is an independent predictor of HAPR and HCPR, and the state of frailty is independently associated with the platelet responses to clopidogrel and aspirin.
The “Sponge sign”: A novel feature of inflammatory choroidal neovascularization
Authors: Giuffrè Chiara , Marchese Alessandro , Fogliato Giovanni , Miserocchi Elisabetta , Modorati Giulio Maria , Sacconi Riccardo , Cicinelli Maria Vittoria , Miere Alexandra , Amoroso Francesca , Capuano Vittorio , Souied Eric , Bandello Francesco , Querques Giuseppe
Published: April 2020

Introduction:

To investigate choroidal thickness changes related to the clinical activity of inflammatory choroidal neovascularization in punctate inner choroidopathy/multifocal choroiditis as compared to myopic choroidal neovascularization.

Materials and methods:

Consecutive inflammatory choroidal neovascularization secondary to punctate inner choroidopathy/multifocal choroiditis, and myopic choroidal neovascularization were retrospectively reviewed. By means of enhanced-depth imaging optical coherence tomography, choroidal thickness was assessed at the same location before choroidal neovascularization development, at choroidal neovascularization onset (baseline), and after treatment.

Results:

Eleven eyes with inflammatory choroidal neovascularization and 11 eyes with myopic choroidal neovascularization were analyzed. Choroidal thickness beneath inflammatory choroidal neovascularization significantly increased at baseline and decreased after therapy (“Sponge sign”), reaching preclinical values. In particular, mean choroidal thickness under inflammatory choroidal neovascularization was 145 ± 85 µm at the preclinical stage, increased to 210 ± 103 µm at baseline (p = 0.006), and decreased to 136 ± 87 µm after treatment (p = 0.017). Conversely, no significant choroidal thickness changes were disclosed in myopic choroidal neovascularization eyes, under any location.

Conclusion:

Optical coherence tomography–based choroidal thickness evaluation may represent an additional useful tool to monitor inflammatory choroidal neovascularization activity. Moreover, choroidal thickness under choroidal neovascularizations could be used to discriminate the origin of choroidal neovascular membrane, either inflammatory or myopic, in doubtful cases and guide the therapeutic management.
Dimethylation of eEF1A at Lysine 55 Plays a Key Role in the Regulation of eEF1A2 on Malignant Cell Functions of Acute Myeloid Leukemia
Authors: Xiao Shan , Wang Yanping , Ma Yuwen , Liu Jue , Tang Can’e , Deng Aiping , Fang Chunxiang
Published: April 2020

Objective:

This study aimed to explore whether eukaryotic translation elongation factor 1 alpha 2 affected cell proliferation, migration, and apoptosis via regulating the dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 in acute myeloid leukemia.

Methods:

The expressions of eukaryotic translation elongation factor 1 alpha 2 and dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 in acute myeloid leukemia cell lines and human normal bone marrow mononuclear cells (as control) were assessed. Control CRISPR-Cas9 lentivirus, eukaryotic translation elongation factor 1 alpha 2 knockout CRISPR-Cas9 lentivirus, vector plasmid, eukaryotic translation elongation factor 1 alpha 2 wild type overexpression plasmid, and eukaryotic translation elongation factor 1 alpha 2 with a K55R substitution overexpression plasmid were transfected into AML-193 and Kasumi-1 cells combined or alone, and were accordingly divided into 4 groups (Sgcontrol + vector group, SgeEF1A2 + vector group, SgeEF1A2 + eEF1A2WT group, and SgeEFIA2 + eEF1A2K55R group).

Results:

Eukaryotic translation elongation factor 1 alpha 2 and dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 expressions were higher in AML-193, Kasumi-1, and KG-1 cell lines compared to the control. In AML-193 and Kasumi-1 cells, the knockout and compensated experiments revealed that eukaryotic translation elongation factor 1 alpha 2 promoted cell proliferation and migration but repressed apoptosis. Additionally, the knockout of eukaryotic translation elongation factor 1 alpha 2 decreased dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 expression, meanwhile, eukaryotic translation elongation factor 1 alpha 2 wild type overexpression enhanced while eukaryotic translation elongation factor 1 alpha 2 with a K55R substitution overexpression did not influence the dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 expression. Furthermore, eukaryotic translation elongation factor 1 alpha 2 wild type overexpression promoted cell proliferation, enhanced migration, and decreased apoptosis, but eukaryotic translation elongation factor 1 alpha 2 with a K55R substitution overexpression did not influence these cellular functions in AML-193 and Kasumi-1 cells, suggesting the implication of dimethylation of eukaryotic translation elongation factor 1 alpha at lysine 55 in eukaryotic translation elongation factor 1 alpha 2 mediated oncogenesis of acute myeloid leukemia.

Conclusion:

Eukaryotic translation elongation factor 1 alpha 2 and its dimethylated product may serve as therapeutic targets, and these findings may provide support for exploring novel strategies in acute myeloid leukemia treatment.
Quantitative measurement properties and score interpretation of the Cough Severity Diary in patients with chronic cough
Authors: Martin Nguyen Allison , Bacci Elizabeth , Dicpinigaitis Peter , Vernon Margaret
Published: April 2020

Aims:

The Cough Severity Diary (CSD) was developed in accordance with the FDA guidance for patient-reported outcome measures and is focused on capturing the patient’s perception of cough in terms of frequency, intensity, and disruption due to their cough. The measure includes a series of seven items asking patients to rate the frequency (three items), intensity (two items), and disruptiveness (two items) of their cough. The instrument was designed to be completed daily before bedtime, has a recall period of ‘today,’ and responses to items are entered on an 11-point numeric rating scale ranging from 0 to 10 with anchors on each end. The objective of this analysis was to confirm the domain structure of the CSD and assess its reliability, validity, and responsiveness in adult patients with refractory or unexplained chronic cough (RCC/UCC). Criteria for defining meaningful changes in mean weekly CSD total and domain scores in the context of a clinical trial were also developed.

Methods:

Pooled data from a phase II randomized controlled trial of an investigational treatment for RCC/UCC were analyzed. Participants were non-smokers, had RCC/UCC for ⩾1 year, and a baseline cough severity visual analogue scale (VAS) ⩾40 mm. CSD scores (baseline, week 4), were analyzed; the Leicester Cough Questionnaire (LCQ), cough severity VAS, Patient Global Impression of Change (PGIC), and objective cough frequency counts were used for validation. CSD domain structure (Total, Frequency, Intensity, Disruption) was assessed for scoring.

Results:

A total of 253 participants were included (mean age 60.2; 76% female). Global fit of the three-factor CSD was acceptable. For the CSD total score, internal consistency (α = 0.89) and test–retest reliability (intraclass correlation coefficient = 0.68) were high. CSD total scores were correlated with the LCQ total ( r = –0.62) and cough severity VAS ( r = 0.84). Participants with a PGIC score of 1 or 2 (most improved groups) had the greatest mean score improvement on the CSD Total (Day 0 to Day 28), supporting responsiveness (similar findings for subscales). A change threshold of ⩾1.3-point reduction on the total and subscale scores is appropriate to define clinically meaningful improvement.

Conclusion:

The CSD is a reliable, valid, and responsive measure of cough symptom severity in patients with refractory or unexplained chronic cough and fit-for-purpose for assessing changes in cough severity in clinical trials. The reviews of this paper are available via the supplemental material section.
Review of the Long-Term Implantable Senseonics Continuous Glucose Monitoring System and Other Continuous Glucose Monitoring Systems
Authors: Joseph Jeffrey I.
Published: April 2020

The article published by Kevin Cowart in this issue of the Journal of Diabetes Science and Technology (JDST) is a detailed overview of the clinical trial data and analysis used to demonstrate the safety and effectiveness of the Eversense continuous glucose monitoring (CGM) System for regulatory approval and clinical acceptance. The article describes the published study results for safety, accuracy, reliability, ease of insertion/removal, adverse events, and ease of diabetes patient-use for controlling their glucose levels short and long term. The author nicely compares Eversense CGM System safety and performance with the short-term subcutaneous tissue CGM systems being commercialized by Dexcom, Medtronic Diabetes, and Abbott Diabetes. This comparison may help the clinician define which type of patient with diabetes might benefit the most from the long-term implantable CGM system. The majority of studied patients describe a positive experience managing their diabetes with the Eversense CGM System and request implantation of a new sensor 90 or 180 days later.
The effect of electronic reminders on the recording of diagnoses in primary care: A quasi-experimental before and after study
Authors: Lehtovuori Tuomo , Heikkinen Anna M , Raina Marko , Kauppila Timo
Published: April 2020

Objectives: This study examined whether using electronic reminders leads to an increase in the rate of diagnosis recordings in the electronic health record system following visits to a general practitioner. The impact of electronic reminders was studied in the primary health care of a Finnish city. Methods: This observational quasi-experimental study based on a before-and-after design was carried out by installing an electronic reminder to improve the recording of diagnoses in the computerized electronic health record system. The quantity of the recorded diagnoses was observed before and after the intervention. The effect of this intervention on the distribution of different diagnoses was also studied. Results: Before intervention, 33%–46% of visits (to general practitioners/month) had recorded diagnose in the primary health care units. After 4 years, the recording rate had risen to 87%–95% (p < 0.001). The rate of change in the recording of diagnoses was highest during the first year of intervention and plateaued about 3.5 years after application reminders. In the present study, most of the visits concerned mild respiratory infections, elevated blood pressure, low back pain and type 2 diabetes. Conclusion: An electronic reminder is likely to improve the recording of diagnoses during the visits to general practitioners. The distribution of diagnoses was in line with former reports concerning diagnoses in Finnish primary care.
Job quality of refugees in Austria: Trade-offs between multiple workplace characteristics
Authors: Ortlieb Renate , Weiss Silvana
Published: April 2020

Do employers tend to exploit refugees or do they offer them high-quality jobs? This article examines the job quality of refugees from Afghanistan and Syria working in Austria. It uses unique survey data of 316 refugees and cluster analysis to identify job quality profiles. Drawing on well-established job quality frameworks, it considers multiple dimensions of job quality, including pay, job security, overqualification in terms of level and content area, learning opportunities, at-home feeling and health aspects. The findings reveal four job quality profiles with considerable trade-offs or compromises between job quality dimensions. Furthermore, the job quality profiles are associated with the methods refugees use to find a job. The study enhances understanding of labour market integration of refugees and the associated role of human resource management.
2SigFinder: the combined use of small-scale and large-scale statistical testing for genomic island detection from a single genome
Authors: Kong Rui , Xu Xinnan , Liu Xiaoqing , He Pingan , Zhang Michael Q. , Dai Qi
Published: April 2020

Background

Genomic islands are associated with microbial adaptations, carrying genomic signatures different from the host. Some methods perform an overall test to identify genomic islands based on their local features. However, regions of different scales will display different genomic features.

Results

We proposed here a novel method “2SigFinder “, the first combined use of small-scale and large-scale statistical testing for genomic island detection. The proposed method was tested by genomic island boundary detection and identification of genomic islands or functional features of real biological data. We also compared the proposed method with the comparative genomics and composition-based approaches. The results indicate that the proposed 2SigFinder is more efficient in identifying genomic islands.

Conclusions

From real biological data, 2SigFinder identified genomic islands from a single genome and reported robust results across different experiments, without annotated information of genomes or prior knowledge from other datasets. 2SigHunter identified 25 Pathogenicity, 1 tRNA, 2 Virulence and 2 Repeats from 27 Pathogenicity, 1 tRNA, 2 Virulence and 2 Repeats, and detected 101 Phage and 28 HEG out of 130 Phage and 36 HEGs in S. enterica Typhi CT18, which shows that it is more efficient in detecting functional features associated with GIs.
Organic mulching positively regulates the soil microbial communities and ecosystem functions in tea plantation
Authors: Zhang Shuning , Wang Yu , Sun Litao , Qiu Chen , Ding Yiqian , Gu Honglian , Wang Linjun , Wang Zhaoshun , Ding Zhaotang
Published: April 2020

Background

Different mulches have variable effects on soil physicochemical characteristics, bacterial and fungal communities and ecosystem functions. However, the information about soil microbial diversity, community structure and ecosystem function in tea plantation under different mulching patterns was limited. In this study, we investigated bacterial and fungal communities of tea plantation soils under polyethylene film and peanut hull mulching using high-throughput 16S rRNA and ITS rDNA gene Illumina sequencing.

Results

The results showed that the dominant bacterial phyla were Proteobacteria, Actinobacteria, Acidobacteria and Chloroflexi, and the dominant fungal phyla were Ascomycota, Mortierellomycota and Basidiomycota in all samples, but different mulching patterns affected the distribution of microbial communities. At the phylum level, the relative abundance of Nitrospirae in peanut hull mulching soils (3.24%) was significantly higher than that in polyethylene film mulching soils (1.21%) in bacterial communities, and the relative abundances of Mortierellomycota and Basidiomycota in peanut hull mulching soils (33.72, 21.93%) was significantly higher than that in polyethylene film mulching soils (14.88, 6.53%) in fungal communities. Peanut hull mulching increased the diversity of fungal communities in 0–20 cm soils and the diversity of bacterial communities in 20–40 cm soils. At the microbial functional level, there was an enrichment of bacterial functional features, including amino acid transport and metabolism and energy production and conversion, and there was an enrichment of fungal functional features, including undefined saprotrophs, plant pathogens and soils aprotrophs.

Conclusions

Unique distributions of bacterial and fungal communities were observed in soils under organic mulching. Thus, we believe that the organic mulching has a positive regulatory effect on the soil bacterial and fungal communities and ecosystem functions, and so, is more suitable for tea plantation.
Cloning and molecular characterization of Triticum aestivum ornithine amino transferase (TaOAT) encoding genes
Authors: Anwar Alia , She Maoyun , Wang Ke , Ye Xingguo
Published: April 2020

Background

Ornithine aminotransferase (OAT, EC:2.6.1.13), alternatively known as ornithine delta aminotransferase (δOAT), is a pyridoxal phosphate (PLP)-dependent enzyme involved in the conversion of ornithine into glutamyl-5-semi-aldehyde (GSA) and vice versa. Up till now, there has been no study on OAT in wheat despite the success of its isolation from rice, maize, and sorghum. This study focuses on identification and molecular characterization of OAT in wheat.

Results

In total, three homeologous OAT genes in wheat genome were found on chromosome group 5, named as TaOAT-5AL, TaOAT-5BL, and TaOAT-5DL. Sequence alignment between gDNA and its corresponding cDNA obtained a total of ten exons and nine introns. A phylogenetic tree was constructed and results indicated that OATs shared highly conserved domains between monocots and eudicots, which was further illustrated by using WebLogo to generate a sequence logo. Further subcellular localization analysis indicated that they functioned in mitochondria. Protein-protein interactions supported their role in proline biosynthesis through interactions with genes, such as delta 1-pyrroline-5-carboxylate synthetase (P5CS) and pyrroline-5-carboxylate reductase (P5CR), involved in the proline metabolic pathway. Promoter analysis exposed the presence of several stress responsive elements, implying their involvement in stress regulation. Expression profiling illustrated that TaOAT was highly induced in the wheat plants exposed to drought or salt stress condition. Upregulated expression of TaOATs was observed in stamens and at the heading stage. A potential role of TaOAT genes during floret development was also revealed. Furthermore, the transgenic plants overexpressing TaOAT showed enhanced tolerance to drought stress by increasing proline accumulation. In addition, salt tolerance of the transgenic plants was also enhanced.

Conclusion

TaOATs genes were involved in proline synthesis and nitrogen remobilization because they interacted with genes related to proline biosynthesis enzymes and arginine catabolism. In addition, TaOAT genes had a role in abiotic stress tolerance and a potential role in floret development. The results of this study may propose future research in the improvement of wheat resistance to abiotic stresses.
Oral health-related quality of life in adult patients with end-stage kidney diseases undergoing renal replacement therapy – a systematic review
Authors: Schmalz Gerhard , Patschan Susann , Patschan Daniel , Ziebolz Dirk
Published: April 2020

Background

The oral health of patients undergoing renal replacement therapy (RRT) is insufficient. Poor oral health and its components can affect the oral health-related quality of life (OHRQoL) of these patients. The aim of this systematic review was to assess the OHRQoL of adult patients under RRT.

Methods

A systematic literature search was performed using the terms: dialysis OR “renal disease” OR kidney OR “renal failure” OR “kidney transplantation” OR hemodialysis OR “peritoneal dialysis” OR “renal replacement therapy” AND “oral health-related quality of life”, complemented by manual search. Clinical studies including adults (age ≥ 18 years) that were published between 2009 and 2019 were included in qualitative analysis.

Results

Twelve out of 20 studies were included in the qualitative analysis. The majority (11/12 studies) included patients undergoing haemodialysis (HD), with a sample size between 47 and 512 participants. Two studies included patients after kidney transplantation. Only one-quarter of the investigations included a healthy control group. The overall OHRQoL was found to be reduced. The majority of studies found relationships between OHRQoL and different oral health parameters. Furthermore, several relationships between OHRQoL and general quality of life as well as disease related parameters including age, gender, diabetes, blood parameters and dialysis duration were found. OHRQoL subscales psychological/psychosocial impairment and pain were predominantly affected.

Conclusions

Patients under RRT suffer from a reduced OHRQoL, which is potentially influenced by oral health and disease related parameters. Interdisciplinary dental care is needed and should consider both physical and psychosocial issues.
Spinal cerebrospinal fluid leak in the context of pars interarticularis fracture
Authors: Chan Tommy Lik Hang , Cowan Robert , Hindiyeh Nada , Hashmi Syed , Lanzman Bryan , Carroll Ian
Published: April 2020

Background

Spinal cerebrospinal fluid (CSF) leak can lead to intracranial hypotension and is an important differential diagnosis to consider in patients with sudden-onset chronic daily headaches. Pars interarticularis (PI) fracture is a potential rare cause of suspected spinal CSF leak.

Methods

This is a retrospective case series of 6 patients with suspected spinal CSF leak evaluated between January 2016 and September 2019. All patients received a magnetic resonance imaging (MRI) of the brain with and without gadolinium, MRI whole spine and full spine computed tomography (CT) myelogram. Targeted epidural patches with fibrin sealant were performed. Treatment response at return visit (3 months post-patch) was documented.

Results

Six patients (4 females, 2 males) were diagnosed with a suspected spinal CSF leak and PI fracture. Mean age at the time of headache onset was 39 years old, and a range from 32 to 50 years old. Mean time to targeted epidural patches with fibrin sealant was 4.5 years. All 6 patients had PI fractures identified on CT myelogram and received targeted epidural patches with fibrin sealant at the site of the PI fracture. All patients had significant improvement in their headache intensity.

Conclusion

Our study highlights: 1) the importance of PI fracture as a possible culprit of suspected spinal CSF leak in patients with intracranial hypotension; 2) the added benefit of CT imaging for detecting bony abnormalities such as fractures in patients with intracranial hypotension; and 3) the successful treatment of suspected spinal CSF leak when targeting the fracture site.
Magnetic resonance-guided focused ultrasound for mesial temporal lobe epilepsy: a case report
Authors: Abe Keiichi , Yamaguchi Toshio , Hori Hiroki , Sumi Masatake , Horisawa Shiro , Taira Takaomi , Hori Tomokatsu
Published: April 2020

Background

We report the first case of transcranial magnetic resonance-guided focused ultrasound (MRgFUS) for mesial temporal lobe epilepsy (MTLE).

Case presentation

The target was located 20 mm lateral from the midline and 15 mm above the skull base (left hippocampus). Despite the application of maximal energy, the ablation temperature did not exceed 50 °C, probably because of the low number of effective transducer elements with incident angles below 25 degrees. The skull density ratio was 0.56. Post-operative magnetic resonance imaging did not reveal any lesion and the patient remained almost seizure-free for up to 12 months.

Conclusions

This preliminary case report suggests that MRgFUS may be effective for treating cases of MTLE. Therefore, the safety and feasibility of MRgFUS should be evaluated in future studies with larger numbers of participants and longer follow-up duration.