bluuebunny/crossref_metadata_2025 huggingface dataset:
H syndrome: Clinical, histological and genetic investigation in Tunisian patients
Authors: Jaouadi Hager , Zaouak Anissa , Sellami Khadija , Messaoud Olfa , Chargui Mariem , Hammami Houda , Jones Meriem , Jouini Raja , Chadli Debbiche Achraf , Chraiet Karima , Fenniche Sami , Mrad Ridha , Mokni Mourad , Turki Hamida , Benkhalifa Rym , Abdelhak Sonia
Published: May 2018

H syndrome is a rare autosomal recessive disorder with characteristic dermatological findings consisting of hyperpigmentation and hypertrichosis patches mainly located on the inner thighs and multisystemic involvement including hepatosplenomegaly, hearing loss, heart abnormalities and hypogonadism. The aim of this study was to conduct a clinical and genetic investigation in five unrelated Tunisian patients with suspected H syndrome. Hence, genetic analysis of the SLC29A3 gene was performed for four patients with a clinical diagnosis of H syndrome. We identified a novel frame‐shift mutation in the SLC29A3 gene in a female patient with a severe clinical presentation. Furthermore, we report two mutations previously described, the p.R363Q mutation in a male patient and the p.P324L mutation in two patients of different age and sex. This paper extends the mutation spectrum of H syndrome by reporting a novel frame‐shift mutation, the p.S15Pfs*86 in exon 2 of SLC29A3 gene and emphasizes the relevance of genetic testing for its considerable implications in early diagnosis and clinical management.
Effects of dietary antioxidant supplementation of feed, milk processing and storage on the lutein content and sensory quality of bovine milk
Authors: Wang Chong , Liu Jianxin , Duan Bruce , Lao Ye , Qi Phoebe X , Ren Daxi
Published: May 2018

The objectives were to investigate the lutein content of bovine milk as affected by the cows' dietary lutein supplementation in the presence or absence of antioxidants, vitamin E (Vit E), tea polyphenols (TP) and ethoxyquin (EQ), and to assess the impact of subsequent pasteurisation on the lutein content and sensory quality of the bovine milk. Among the dietary supplement formulations studied, lutein plus Vit E was the most effective, lutein plus TP was moderately effective, and lutein plus EQ was adversely effective at improving the milk's lutein content by 53–88%, relative to the basal diet. Co‐supplementation with antioxidant also mitigated the lutein loss caused by the milk's processing and storage. This study showed that supplementation of bovine feed with Vit E plus lutein resulted in milk with high lutein content, and a superior ability to resist heat‐induced lutein loss and sensory changes.
Identification and functional characterization of doublesex gene in the testis of Spodoptera litura
Authors: Du Qian , Wen Liang , Zheng Si‐Chun , Bi Hong‐Lun , Huang Yong‐Ping , Feng Qi‐Li , Liu Lin
Published: May 2018

Fusion of the testis occurs in most Lepidoptera insects, including Spodoptera litura, an important polyphagous pest. Testicular fusion in S. litura is advantageous for male reproduction, and the molecular mechanism of fusion remains unknown. Doublesex influences the formation of genitalia, the behavior of courtship, and sexually dimorphic traits in fruit‐fly and silkworm, and is essential for sexual differentiation. However, its purpose in the testis of S. litura remains unknown. The doublesex gene of S. litura (Sldsx) has male‐specific SldsxM and female‐specific SldsxF isoforms, and exhibits a higher expression level in the male testis. At the testicular fusion stage (L6D6), Sldsx attained the highest expression compared to the pre‐fusion and post‐fusion periods. Moreover, Sldsx had a higher expression in the peritoneal sheaths of testis than that of germ cells in the follicle. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) was applied to S. litura to determine the role of Sldsx. A mixture of single guide RNA messenger RNA and Cas9 protein (300 ng/μL each) was injected into eggs within 2 h following oviposition. CRISPR/Cas9 successfully induced genomic mutagenesis of Sldsx at G0 generation. The mutant males had smaller testis surrounded by less tracheae. Moreover, the mutant males had abnormal external genitalia and could not finish mating with wild‐type females. Additionally, testes were fused for almost all mutant males. The results showed that Sldsx was not related to testicular fusion, and is required for both testis development and the formation and function of external genitalia in S. litura. The main roles of doublesex on the male are similar to other insects.
Pathologic study of tumour extension for clinically localized unilateral nasopharyngeal carcinoma: Should the contralateral side be included in the clinical target volume?
Authors: Li An‐chuan , Zhang Ying‐ying , Zhang Chi , Wang De‐sheng , Xu Ben‐hua
Published: May 2018

Introduction

The clinical target volume (CTV) delineation is crucial for tumour control and normal tissue protection. This study investigated the contralateral extension of nasopharyngeal carcinoma (NPC) in patients with a clinically diagnosed unilateral tumour to pursue the possibility of CTV reduction.

Methods

Twenty NPC patients with localized tumours confined to only one side of the nasopharynx as shown by magnetic resonance imaging and fibreoptic endoscopy were selected for biopsy. The tissues of the contralateral pharyngeal recess (CPR) and the contralateral posterosuperior wall (CPSW) of the nasopharynx were obtained in each case and prepared for pathological examination. The factors associated with contralateral tumour infiltration were analysed.

Results

Five of 20 (25.0%) patients were pathologically confirmed to have carcinoma cell infiltration in the CPSW, including 2 (10.0%) that had carcinoma cell infiltration in the CPR. The T classification (P = 0.014) and primary tumour volume (P = 0.033) were positively associated with the infiltration of the CPSW, but none of the primary tumour factors affected the involvement of the CPR. The contralateral retropharyngeal lymph node (LN) metastasis (P = 0.016), but not the contralateral cervical LN, was significantly associated with the infiltration of the CPR. Positive Epstein–Barr virus DNA (EBV‐DNA) was another factor that increased the probability of CPR invasion (P = 0.044).

Conclusions

Contralateral pharyngeal recess infiltration is rare in patients with clinically diagnosed unilateral primary NPC. Reduced CTV coverage, including the CPSW but not CRP, is feasible for patients with unilateral cancer of the nasopharynx without contralateral LN metastasis or positive EBV‐DNA. Further large‐sample studies are needed.
Cigarette brand diversity and price changes during the implementation of plain packaging in the United Kingdom
Authors: Breton Magdalena Opazo , Britton John , Huang Yue , Bogdanovica Ilze
Published: May 2018

Background and aim

Plain packaging of cigarettes appeared in the United Kingdom in July 2016 and was ubiquitous by May 2017. The change coincided with another legislative change, raising the minimum pack size from 10 to 20 cigarettes. Laws imposing plain packaging on cigarette packs remove another promotional route from tobacco companies, but the effect of such laws on brand diversity, pricing and sales volume is unknown. This study aimed to (1) describe and quantify changes in brand diversity, price segmentation and sales volumes and (2) estimate the association between the introduction of plain cigarette packaging and cigarette pricing in the United Kingdom.

Design

We used a natural experiment design to assess the impact of plain packaging legislation on brand diversity and cigarette prices. The data comprised a sample of 76% of sales of cigarettes in the UK between March 2013 and June 2017.

Setting

United Kingdom.

Measurements

Cigarette prices, number of brands and products and volumes of sales.

Findings

During the period analysed, there was a slight decrease in the number of cigarette brands. There was also an initial increase observed in the number of cigarette products, due mainly to an increase in the number of products in packs of fewer than 20 cigarettes sold before July 2016, which was then followed by a rapid decrease in the number of products that coincided with the implementation of the new legislation. Cigarette sales volumes during this period did not deviate from the preceding secular trend, but prices rose substantially. Regression results showed that price per cigarette, regardless of pack size, was 5.0 [95% confidence interval (CI) = 4.8–5.3] pence higher in plain than in fully branded packs. For packs of 20 cigarettes, price increases were greater in the lower price quintiles, ranging from 2.6 (95% CI = 2.4–2.7) GBP in the lowest to 0.3 (95% CI = 0.3–0.4) GBP per pack in the highest quintile.

Conclusions

The implementation of standardized packaging legislation in the United Kingdom, which included minimum pack sizes of 20, was associated with significant increases overall in the price of manufactured cigarettes, but no clear deviation in the ongoing downward trend in total volume of cigarette sales.
A comparison of hydroxyl ion diffusion through root dentine from various calcium hydroxide preparations
Authors: Plataniotis Emanuel , Abbott Paul
Published: May 2018

Different calcium hydroxide [Ca(OH)2] formulations are available. The aim was to assess differences in hydroxyl ion release with different formulations. Sixty‐six teeth were divided one control (n = 6) and four experimental (n = 15) groups: (i) Pulpdent Paste; (ii): DT Temporary Dressing; (iii): Ca(OH)2 powder/saline; (iv): Ca(OH)2 points; (v): no medicament. pH was measured in inner dentine and outer dentine cavities over 12 weeks. Inner dentine pH rose rapidly for all groups except the points and controls. Peak pH was reached by day 2 before dropping and stabilising (8.0–9.2). Outer dentine pH rose steadily until day 21 for aqueous solutions and then stabilised (8.0–8.5). The points had minimal pH increase for the entire period. There were no significant differences in hydroxyl ion release between the aqueous solutions. Type of paste base did not affect release and diffusion of hydroxyl ions which continued for 84 days.
Efficiency of a pedometer device for detecting estrus in standing heat and silent heat in Japanese Black cattle
Authors: Hojo Takuo , Sakatani Miki , Takenouchi Naoki
Published: May 2018

The usefulness of a radiotelemetric pedometer for estrus detection in standing (ST) heat, or in silent heat without ST events, but in which ovulation is observed, in Japanese Black cattle was investigated. The duration of an increase in steps in ST heat was 11.8 ± 1.3 hr, and it was similar to that of ST events (duration: 10.1 ± 0.8 hr). Even in silent heat, the change pattern and the duration (11.6 ± 0.2 hr) of the period with an increase in steps during estrus were not different compared with ST heat. When artificial insemination (AI) was performed at 15.5 ± 0.6 hr from the onset of estrus detected by the pedometer in ST heat cases, the conception rate was 57.1% (8/14). Furthermore, fertility in cattle that underwent silent heat was evaluated. When AI was performed at 14.4 ± 2.0 hr from the onset of estrus detected by the pedometer, the conception rate was 60% (3/5) in silent heat cases. The overall results suggest that the radiotelemetric pedometer is a valid device for detecting estrus and it can even detect silent heat in Japanese Black cattle. Moreover, even silent heat cattle are fertile when AI is performed at the appropriate time.
Evaluation of three different patterns of feed intake during early lactation in lactating sows
Authors: Lei Xin Jian , Lee Sang In , Kim In Ho
Published: May 2018

Forty‐five multiparous sows (Landrace × Yorkshire; parity: 3.58 ± 1.30) were used to determine the effects of three patterns of feed intake during early lactation on the performance of lactating sows. Experimental treatments were as follows: IFI‐1.4, the amount of feed increased by 1.4 kg per day for the first 5 days post‐farrowing, followed by ad libitum feeding until weaning; IFI‐1.0, the amount of feed increased by 1.0 kg per day for the first 7 days post‐farrowing, followed by ad libitum feeding until weaning; IFI‐0.7, the amount of feed increased by 0.7 kg per day for the first 10 days post‐farrowing, followed by ad libitum feeding until weaning. The number of live piglets at birth/litter in three dietary treatments was 11.50, 10.07, and 10.85, respectively. Sows in the IFI‐1.4 treatment had lower backfat loss during lactation, greater daily feed intake during days 1–6, 7–12, and 1–24 compared with those in the IFI‐0.7 treatment (p < .05). The litter weaning weight, litter weight gain, and average litter daily gain in the IFI‐1.4 treatment were greater compared with those in the IFI‐0.7 treatment (p < .05). In conclusion, the results indicated that the IFI‐1.4 feed intake pattern allowed lactating sows and their litters to obtain optimal performance.
Elotuzumab monotherapy in patients with smouldering multiple myeloma: a phase 2 study
Authors: Jagannath Sundar , Laubach Jacob , Wong Ellice , Stockerl‐Goldstein Keith , Rosenbaum Cara , Dhodapkar Madhav , Jou Ying‐Ming , Lynch Mark , Robbins Michael , Shelat Suresh , Anderson Kenneth C. , Richardson Paul G.
Published: May 2018

Smouldering multiple myeloma (SMM) is associated with increased risk of progression to multiple myeloma within 2 years, with no approved treatments. Elotuzumab has been shown to promote natural killer (NK) cell stimulation and antibody‐dependent cellular cytotoxicity (ADCC) in vitro. CD56dim (CD56dim/CD16+/CD3−/CD45+) NK cells represent the primary subset responsible for elotuzumab‐induced ADCC. In this phase II, non‐randomized study (NCT01441973), patients with SMM received elotuzumab 20 mg/kg intravenously (cycle 1: days 1, 8; monthly thereafter) or 10 mg/kg (cycles 1, 2: weekly; every 2 weeks thereafter). The primary endpoint was the relationship between baseline proportion of bone marrow‐derived CD56dim NK cells and maximal M protein reduction; secondary endpoints included overall response rate (ORR) and progression‐free survival (PFS). Fifteen patients received 20 mg/kg and 16 received 10 mg/kg; combined data arepresented. At database lock (DBL, September 2014), no association was found between baseline CD56dim NK cell proportion and maximal M protein reduction. With minimum 28 months' follow‐up (DBL: January 2016), ORR (90% CI) was 10% (2·7–23·2) and 2‐year PFS rate was 69% (52–81%). Upper respiratory tract infections occurred in 18/31 (58%) patients. Four (13%) patients experienced infusion reactions, all grade 1–2. Elotuzumab plus lenalidomide/dexamethasone is under investigation for SMM.
How we manage Gaucher Disease in the era of choices
Authors: Revel‐Vilk Shoshana , Szer Jeff , Mehta Atul , Zimran Ari
Published: May 2018

Treatment of Gaucher Disease (GD) is now beset with the abundance of therapeutic options for an individual patient, making the choice of therapy complex for both expert and non‐expert clinicians. The pathogenesis of all disease manifestations is a gene mutation‐driven deficiency of glucocerebrosidase, but the clinical expression and response of each of the clinical manifestations to different therapies can be difficult to predict. Enzyme replacement therapy has been available since 1991 and is well‐established, with known efficacy and minimal toxicity. Of interest, the three available enzymes are distinct molecules and were registered as new products, not biosimilars. Oral substrate reduction therapy has undergone a revitalisation with a newly approved agent in this class for which some efficacy and toxicity questions have been raised. Herein we present our approach to the management of GD in the era of choices, including a new algorithm for how to manage a newly diagnosed patient.
Human Pegivirus infection and lymphoma risk and prognosis: a North American study
Authors: Fama Angelo , Xiang Jinhua , Link Brian K. , Allmer Cristine , Klinzman Donna , Feldman Andrew L. , Nowakowski Grzegorz S. , Liebow Mark , Larson Melissa C. , Maurer Matthew J. , Ansell Stephen M. , Novak Anne J. , Asmann Yan W. , Slager Susan L. , Call Timothy G. , Habermann Thomas M. , Cerhan James R. , Stapleton Jack T.
Published: May 2018

We evaluated the association of Human Pegivirus (HPgV) viraemia with risk of developing lymphoma, overall and by major subtypes. Because this virus has also been associated with better prognosis in the setting of co‐infection with human immunodeficiency virus, we further assessed the association of HPgV with prognosis. We used risk factor data and banked plasma samples from 2094 lymphoma cases newly diagnosed between 2002 and 2009 and 1572 frequency‐matched controls. Plasma samples were tested for HPgV RNA by reverse transcription polymerase chain reaction (RT‐PCR), and those with RNA concentrations <5000 genome equivalents/ml were confirmed using nested RT‐PCR methods. To assess the role of HPgV in lymphoma prognosis, we used 2948 cases from a cohort study of newly diagnosed lymphoma patients (included all cases from the case‐control study). There was a positive association of HPgV viraemia with risk of lymphoma overall (Odds ratio = 2·14; 95% confidence interval [CI] 1·63–2·80; P < 0·0001), and for all major subtypes except Hodgkin lymphoma and chronic lymphocytic leukaemia/small lymphocytic lymphoma, and this was not confounded by other lymphoma risk factors. In contrast, there was no association of HPgV viraemia with event‐free survival (Hazard ratio [HR] = 1·00; 95% CI 0·85–1·18) or overall survival (HR = 0·97; 95% CI 0·79–1·20) for lymphoma overall, or any of the subtypes. These data support the hypothesis for a role of HPgV in the aetiology of multiple lymphoma subtypes.
Long‐term survival after childhood acute lymphoblastic leukaemia: population‐based trends in cure and relapse by clinical characteristics
Authors: Smith Lesley , Glaser Adam W. , Kinsey Sally E. , Greenwood Darren C. , Chilton Lucy , Moorman Anthony V. , Feltbower Richard G.
Published: May 2018

‘Cure models’ offer additional information to traditional epidemiological approaches to assess survival for cancer patients by simultaneously estimating the proportion cured and the survival of those ‘uncured’. The proportion cured is a summary of long‐term survival while the median survival time of the uncured provides important information on those who are not long‐term survivors. Population‐based trends in the cure proportion and survival of the uncured for childhood acute lymphoblastic leukaemia (ALL) by clinical prognostic risk factors were estimated using flexible parametric cure models, based on overall survival and event‐free survival. Children aged 1–17 years diagnosed between 1990 and 2011 in Yorkshire, UK, were included (n = 492). The percentage cured increased from 77% (95% confidence interval 70–84%) in 1990–1997 to 89% (84–93%) in 2003–2011, while the median survival time of the uncured decreased from 3·2 years (2·2–4·1 years) to 0·7 years (0–1·5 years). Models based on event‐free survival showed a similar trend. The 5‐year cumulative incidence of relapse substantially decreased from 35% in 1990–97 to 9% in 2003–2011. These results show selective improvement in survival between 1990 and 2011 with a significant reduction in the risk of relapse alongside a reduced absolute duration of survival for those destined to be uncured.
PiViewer: An open‐source tool for automated detection and display of π–π interactions
Authors: Liu Ying , Ao Xiang , Wang Qiong , Wang Jianxun , Ge Hu
Published: May 2018

π–π interactions are common and important noncovalent interactions that contribute to biochemical molecular interactions, but the tools for the convenient 3D visualization of π–π interactions are lacking. We have developed an open‐source and easy‐to‐use tool for the automated identification and display of π–π stacking in 3D. It can percept the aromaticity of rings from any selected ligand and the surrounding residues, calculate the distances and dihedral angles between each pair of aromatic ring planes, as well as can highlight the various configurations of π–π interactions in 3D: the sandwich configuration, the T‐shaped configuration, and the parallel‐displaced configuration. In addition, the users can easily adjust or set their own criteria for π–π stacking.
Intrinsically disordered protein‐specific force field CHARMM36IDPSFF
Authors: Liu Hao , Song Dong , Lu Hui , Luo Ray , Chen Hai‐Feng
Published: May 2018

Intrinsically disordered proteins (IDPs) are closely related to various human diseases. Because IDPs lack certain tertiary structure, it is difficult to use X‐ray and NMR methods to measure their structures. Therefore, molecular dynamics simulation is a useful tool to study the conformer distribution of IDPs. However, most generic protein force fields were found to be insufficient in simulations of IDPs. Here, we report our development for the CHARMM community. Our residue‐specific IDP force field (CHARMM36IDPSFF) was developed based on the base generic force field with CMAP corrections for all 20 naturally occurring amino acids. Multiple tests show that the simulated chemical shifts with the newly developed force field are in quantitative agreement with NMR experiment and are more accurate than the base generic force field. Comparison of J‐couplings with previous work shows that CHARMM36IDPSFF and its corresponding base generic force field have their own advantages. In addition, CHARMM36IDPSFF simulations also agree with experiment for SAXS profiles and radii of gyration of IDPs. Detailed analysis shows that CHARMM36IDPSFF can sample more diverse and disordered conformers. These findings confirm that the newly developed force field can improve the balance of accuracy and efficiency for the conformer sampling of IDPs.
Safety and effectiveness of primary transscleral diode laser cyclophotoablation for glaucoma in Nigeria
Authors: Abdull Mohammed M , Broadway David C , Evans Jennifer , Kyari Fatima , Muazu Fatima , Gilbert Clare
Published: May 2018

Importance

To investigate the safety, effectiveness and follow‐up rates after transscleral diode laser cyclophotocoagulation as primary treatment for seeing eyes with primary open angle glaucoma in Bauchi, Nigeria.

Background

There is a high prevalence of primary open angle glaucoma in Africa where adherence to medical treatment and acceptance of surgery are poor.

Design

Prospective case series.

Participants

New glaucoma patients where surgical intervention was recommended.

Methods

A diode 810 nm laser G‐probe was used under retrobulbar anaesthesia to deliver approximately 20 shots for 2000 ms, titrating the power. If both eyes were treated the first was the study eye. Repeat treatment offered if the intraocular pressure (IOP) was >21 mmHg on two consecutive visits.

Main Outcome Measures

IOP < 22 mmHg, change in ≥2 lines of Snellen visual acuity (VA), and complications.

Results

201 out of 204 eyes with complete data analysed. Mean age 52 years, 17 (8.3%) eyes were re‐treated. Mean pre‐treatment IOP was 39 (SD 11) mmHg. 106 (53%) attended at 12 months when the mean IOP was 19 (7–45) mmHg; 77 (73%) had IOP < 22 mmHg. VAs were better in 13 (12.3%) and worse in 23 (21.7%) eyes. Postoperative complications included mild uveitis (5.5%), corneal oedema (2.5%), severe uveitis (0.5%) and transient hypotony (2.0%). No hypotony at 12 months.

Conclusions and Relevance

Transscleral diode laser cyclophotocoagulation controlled IOP in almost three quarters of eyes at 12 months with short‐term preservation of vision and minimal complications. Poor follow‐up in this setting highlights the need for an effective, safe and acceptable treatment where regular follow‐up is less critical.
Utilization of child helpline (CHL) among adolescents in Saudi Arabia: Results from a national survey
Authors: Al‐Eissa Majid A.
Published: May 2018

This study determines the rate of adolescents' interest in disclosing their exposure to abuse or neglect through a child helpline (CHL) and identifies the factors influencing disclosure through the line. A cross‐sectional, national survey utilizing ISPCAN Child Abuse Screening Tool‐Children's Home Version was conducted in secondary high schools in the main 5 provinces of Saudi Arabia. Boys and girls from public and private schools were selected to participate. Students (N = 16,010) aged 15–18 years were invited to complete the survey. The relationship between willingness to utilize CHL and demographic characteristics of the participants were evaluated using chi‐square test. Approximately, half (48%) of the participants reported their willingness to utilize a future CHL if they experienced any type of abuse in the past. Significant difference in age, gender, areas of residence, and abuse experience were found in terms of willingness to utilize CHL, and it was more likely among older age (17–18 years), boys, urban area, and abused participants (p < .01). Participants who reported experiencing multiple forms of abuse, neglect, and psychological abuse were 1.3, 1.9, and 1.2 times, respectively, more likely willing to utilize CHL. Educating adolescents about the utilization of CHL through awareness‐raising activities might reduce the prevalence of child abuse.
MiR‐181b inhibits P38/JNK signaling pathway to attenuate autophagy and apoptosis in juvenile rats with kainic acid‐induced epilepsy via targeting TLR4
Authors: Wang Li , Song Li‐Fang , Chen Xiao‐Yi , Ma Yan‐Li , Suo Jun‐Fang , Shi Jing‐He , Chen Guo‐Hong
Published: May 2018

Objective

To explore the role of miR‐181b in alterations of apoptosis and autophagy in the kainic acid (KA)‐induced epileptic juvenile rats via modulating TLR4 and P38/JNK signaling pathway.

Methods

Dual‐luciferase reporter assay was performed to testify the targeting relationship between miR‐181b and TLR4. After intracerebroventricular injection (i.c.v.) of KA, rats were injected with miR‐181b agomir and TLR4 inhibitor (TAK‐242). The TLR‐4 activator lipopolysaccharide (LPS) was also administered into rats immediately after injection with miR‐181b agomir. Quantitative real‐time‐polymerase chain reaction (qRT‐PCR) was used for detections of miR‐181b and TLR4 expressions, hematoxylin‐eosin (HE) and Nissl staining for observation of the hippocampus morphological changes, and TUNEL staining for apoptosis analysis. Moreover, western blot was determined to detect TLR4 and P38/JNK pathway proteins, as well as autophagy‐ and apoptosis‐related proteins.

Results

TLR4 was identified as a direct target of miR‐181b using Dual‐luciferase reporter assay. KA rats injected with miR‐181b agomir or TAK‐242 had improved learning and memory abilities, reduced seizure severity of Racine’s scale, and lessened neuron injury. Additionally, miR‐181b agomir or TAK‐242 could significantly inhibit P38/JNK signaling, decrease LC3II/I, Beclin‐1, ATG5, ATG7, ATG12, Bax, and cleaved caspases‐3, but increase p62 and Bcl‐2 expression. No significances were found between KA group and KA + miR‐181b + LPS group.

Conclusion

MiR‐181b could inhibit P38/JNK signaling pathway via targeting TLR4, thereby exerting protective roles in attenuating autophagy and apoptosis of KA‐induced epileptic juvenile rats.
Endoscopic ultrasound‐through‐the‐needle biopsy in pancreatic cystic lesions: A multicenter study
Authors: Barresi Luca , Crinò Stefano F. , Fabbri Carlo , Attili Fabia , Poley Jan W. , Carrara Silvia , Tarantino Ilaria , Bernardoni Laura , Giovanelli Silvia , Di Leo Milena , Manfrin Erminia , Tacelli Matteo , Bruno Marco J. , Traina Mario , Larghi Alberto
Published: May 2018

Background and Aim

Tissue acquisition in pancreatic cystic lesions (PCL) is the ideal method for diagnosis and risk stratification for malignancy of these lesions. Direct sampling from the walls of PCL with different devices has shown better results than cytology from cystic fluid. We carried out a retrospective, multicenter study to evaluate the feasibility, safety, and diagnostic yield of a micro‐forceps, specifically designed to be used through a 19‐gauge needle after endoscopic ultrasonography (EUS)‐guided puncture of PCL.

Methods

We retrospectively collected data from patients who underwent EUS‐through‐the‐needle biopsy (EUS‐TTNB) in PCL at six referral centers.

Results

The sampling procedure was carried out in 56 patients (mean age 57.5 ± 13.1 years, M:F 17:39), and was technically successful in all of them (100%; 95% confidence interval [CI], 94–100%). Adverse events occurred in 9/56 (16.1%; 95% CI, 8–28%) patients, with self‐limited intracystic hemorrhage the most common (7/56, 12.5%; 95% CI, 5–24%). All adverse events were mild, and resolved without any specific intervention. Specimens were considered adequate for histological diagnosis in 47/56 (83.9%; 95% CI, 72–92%). In two of these patients, despite the histological adequacy, a diagnosis could not be reached. In two other cases, a specimen sufficient for a cytological diagnosis was obtained. Overall diagnostic yield by combining cytological and histological samples was 47/56 (83.9%; 95% CI, 72–92%).

Conclusion

EUS‐TTNB with micro‐forceps in PCL is feasible, safe, and has a high diagnostic yield. Future prospective studies are needed to better assess the clinical impact of EUS‐TTNB on the management of PCL.
Advanced imaging of an incomplete fracture and exostosis of the third metacarpal bone in a young Warmblood horse
Authors: Isgren C. M. , Maddox T. W. , Blundell R. , Sinovich M. , Rubio‐Martinez L. M.
Published: May 2018

A 5‐year‐old Hanoverian gelding with acute moderate right forelimb lameness underwent nuclear scintigraphy, which identified marked increase in radiopharmaceutical uptake of the proximomedial aspect of the right metacarpus. Ultrasonography and radiography identified sclerosis and exostosis of the proximomedial aspect of the metacarpal boneIIIin‐between the suspensory ligament and the second metacarpal bone, and presence of a suspected fracture. The owner requested euthanasia of the gelding, and post‐mortem computed tomography and magnetic resonance imaging identified marked bone sclerosis and thickening of the palmar cortex of the metacarpalIII(McIII), presence of an incomplete fracture in the palmar cortex of McIII and expansive exostosis from McIII extending in a palmar direction between the suspensory ligament and the second metacarpal bone. Histopathological examination confirmed the imaging findings of sclerosis and led to final diagnosis of a fracture of the palmar cortex of the McIII associated with an exostosis encroaching the medial aspect of the suspensory ligament. No abnormalities were present in the suspensory ligament or metacarpal boneII(McII).
Echocardiographic identification of atrial‐related structures and vessels in horses validated by computed tomography of casted hearts
Authors: Vandecasteele T. , Cornillie P. , van Steenkiste G. , Vandevelde K. , Gielen I. , Vanderperren K. , van Loon G.
Published: May 2018

Background

Echocardiography is the imaging technique of choice for the equine heart. Nevertheless, knowledge about ultrasonographic identification of dorsally located structures and vessels, related to the atria, in horses is scarce.

Objectives

To describe the echocardiographic approach and the identification of structures and vessels in relation to the atria in healthy horses.

Methods

CT images from two equine hearts, casted with self‐expanding foam, were segmented and used to identify atrial‐related structures and vessels. These images were compared with standard and nonstandard ultrasound images from ten healthy horses obtained from a left and right parasternal view optimised to visualise the dorsal cardiac area.

Results

On new standard ultrasound views, specific atrial anatomical landmarks such as vena cava, pulmonary arteries, intervenous tubercle and oval fossa were identified in all horses. In addition, ultrasound views were defined to visualise the brachiocephalic trunk, pulmonary veins and their ostia. The 3D segmented CT images from casted hearts were used to reconstruct slices that corresponded with the echocardiographic images and allowed correct identification of specific structures.

Main limitations

Ultrasound examinations and casts were from different animals. A small number of casts and horses were used; therefore, anatomical variation or individual differences in identifying structures on ultrasound could not be assessed.

Conclusions

Important cardiac structures and vessels, even the different pulmonary veins, could be identified on standard and nonstandard ultrasound images in adult horses. This knowledge is important to guide and develop interventional cardiology and might be useful for diagnostic and therapeutic purposes.
Effects of knee taping during functional activities in older people with knee osteoarthritis: A randomized controlled clinical trial
Authors: Park Kyue‐nam , Kim Si‐hyun
Published: May 2018

Aim

The purpose of the present study was to examine the effects of knee taping on perceived pain, difficulty and stability during functional activities (squat, step‐down, and stair up and down) in older adults with knee osteoarthritis (OA).

Methods

A total of 50 older participants with knee OA participated in this study. The experimental group received non‐elastic taping (NET) and the control group received sham taping. Perceived knee pain on an 11‐point scale (0 = no pain, 10 = worst pain), and difficulty (1 = no difficulty, 5 = extreme difficulty) and stability (1 = no unstable, 5 = extreme unstable) on a 5‐point scale during functional tests (squat, step‐down test, and stair up and down) were reported at baseline and post‐taping application.

Results

At post‐taping, pain intensity, difficulty and stability during squat, step‐down test, and stair up and down were significantly improved in the NET group. In the control group, there was significant decreased pain intensity during squat and stair up and down; however, there were no significant change of other variables. Compared with the control group, the NET significantly improved perceived pain, difficulty and stability during all tasks after the taping application.

Conclusions

The present study showed that NET application can improve perceived pain intensity, difficulty and stability during functional tests in older adults with knee OA. NET can be recommended to improve pain, stability and difficulty during squat, step‐down, and stair up and down in older adults with OA. Geriatr Gerontol Int 2018; 18: 1206–1210.
Lithium Isotope Composition of Marine Biogenic Carbonates and Related Reference Materials
Authors: Bastian Luc , Vigier Nathalie , Reynaud Stéphanie , Kerros Marie‐Emmanuelle , Revel Marie , Bayon Germain
Published: May 2018

In this study, the accuracy and the precision corresponding to Li isotopic measurements of low level samples such as marine and coastal carbonates are estimated. To this end, a total of fifty‐four analyses of a Li‐pure reference material (Li7‐N) at concentrations ranging from 1 to 6 ng ml−1 were first performed. The average δ7Li values obtained for solutions with and without chemical purification were 30.3 ± 0.4‰ (2s, n = 19) and 30.2 ± 0.4‰ (2s, n = 36), respectively. These results show that the chosen Li chemical extraction and purification procedure did not induce any significant isotope bias. Two available carbonate reference materials (JCt‐1 and JCp‐1) were analysed, yielding mean δ7Li values of 18.0 ± 0.27‰ (2s, n = 6) and 18.8 ± 1.8‰ (2s, n = 9), respectively. Small powder aliquots (< 15 mg) of JCp‐1 displayed significant isotope heterogeneity and we therefore advise favouring JCt‐1 for interlaboratory comparisons. The second part of this study concerns the determination of δ7Li value for biogenic carbonate samples. We performed a total of twenty‐nine analyses of seven different tropical coral species grown under controlled and similar conditions (24.0 ± 0.1 °C). Our sample treatment prior to Li extraction involved removal of organic matter before complete dissolution in diluted HCl. Our results show (a) a constant δ7Li within each skeleton and between the different species (δ7Li = 17.3 ± 0.7‰), and (b) a Li isotope fractionation of −2‰ compared with inorganic aragonite grown under similar conditions. Comparison with literature data suggests a significant difference between samples living in aquaria and those grown in natural conditions. Finally, we investigate ancient (fossil) carbonate material and foraminifera extracted from marine sedimentary records. Different leaching procedures were tested using various HCl molarities. Results indicate that carbonate preferential dissolution must be carried out at an acid molarity < 0.18 mol l−1. Possible contamination from silicate minerals can be verified using the Al/Ca ratio, but the threshold value strongly depends on the carbonate δ7Li value. When the silicate/carbonate ratio is high in the sediment sample (typically > 2), contamination from silicates cannot be avoided, even at low HCl molarity (⪡ 0.1 mol l−1). Finally, bulk carbonate and foraminifera extracted from the same core sample exhibited significant discrepancies: δ7Li values of foraminifera were more reproducible but were significantly lower. They were also associated with lower Sr/Ca and higher Mn/Ca ratios, suggesting a higher sensitivity to diagenesis, although specific vital effects cannot be fully ruled out.
Effect of milling method on the chemical composition and antioxidant capacity of Tartary buckwheat flour
Authors: Liu Fang , He Caian , Wang Lijing , Wang Min
Published: May 2018

Tartary buckwheat has attracted increasing attention as a potentially functional food source because of its bioactive components and health benefits. However, little information is available concerning the effect of different milling methods (ultrafine milling (UM), roller milling (RM), stone milling (SM) and wet milling (WM)) on the chemical composition and antioxidant capacity of Tartary buckwheat flour. UM had an important role in reducing the average particle size and in brightening the colour of flour. SM and UM flour exhibited the largest amounts of crude protein, fibre and minerals. WM flour had the lowest moisture content. Rutin was the dominant phenolic compound in UM flour, SM flour and RM flour, whereas quercetin was the most abundant phenolic compound in WM flour. UM flour and SM flour contained higher total phenolic content and antioxidant activity as compared with those of RM flour and WM flour.
Fair draws for group rounds in sport tournaments
Authors: Laliena Pablo , López F. Javier
Published: May 2018

We propose two draw systems for the group round of sport tournaments where there are some geographical and/or seeding restrictions. One of the systems, related to the equal‐sum partition problem, is “perfect,” since it yields perfectly balanced groups. The other system, which uses the classical scheme of extracting teams from pots, is heuristic and gives results where the groups have very similar scores. We apply our results to Fédération Internationale de Football Association (FIFA) Soccer World Cups and show that our proposals are much better than the FIFA system and also outperform other recently developed systems.
The impact of using old germplasm on genetic merit and diversity—A cattle breed case study
Authors: Eynard Sonia E. , Windig Jack J. , Hulsegge Ina , Hiemstra Sipke‐Joost , Calus Mario P. L.
Published: May 2018

Artificial selection and high genetic gains in livestock breeds led to a loss of genetic diversity. Current genetic diversity conservation actions focus on long‐term maintenance of breeds under selection. Gene banks play a role in such actions by storing genetic materials for future use and the recent development of genomic information is facilitating characterization of gene bank material for better use. Using the Meuse‐Rhine‐Issel Dutch cattle breed as a case study, we inferred the potential role of germplasm of old individuals for genetic diversity conservation of the current population. First, we described the evolution of genetic merit and diversity over time and then we applied the optimal contribution (OC) strategy to select individuals for maximizing genetic diversity, or maximizing genetic merit while constraining loss of genetic diversity. In the past decades, genetic merit increased while genetic diversity decreased. Genetic merit and diversity were both higher in an OC scenario restricting the rate of inbreeding when old individuals were considered for selection, compared to considering only animals from the current population. Thus, our study shows that gene bank material, in the form of old individuals, has the potential to support long‐term maintenance and selection of breeds.
Methylation‐reprogrammed Wnt/β‐catenin signalling mediated prenatal hypoxia‐induced brain injury in foetal and offspring rats
Authors: Zhang Yingying , Zhang Mengshu , Li Lingjun , Wei Bin , He Axin , Lu Likui , Li Xiang , Zhang Lubo , Xu Zhice , Sun Miao
Published: May 2018

Prenatal hypoxia (PH) is a common pregnancy complication, harmful to brain development. This study investigated whether and how PH affected Wnt pathway in the brain. Pregnant rats were exposed to hypoxia (10.5% O2) or normoxia (21% O2; Control). Foetal brain weight and body weight were decreased in the PH group, the ratio of brain weight to body weight was increased significantly. Prenatal hypoxia increased mRNA expression of Wnt3a, Wnt7a, Wnt7b and Fzd4, but not Lrp6. Activated β‐catenin protein and Fosl1 expression were also significantly up‐regulated. Increased Hif1a expression was found in the PH group associated with the higher Wnt signalling. Among 5 members of the Sfrp family, Sfrp4 was down‐regulated. In the methylation‐regulating genes, higher mRNA expressions of Dnmt1 and Dnmt3b were found in the PH group. Sodium bisulphite and sequencing revealed hyper‐methylation in the promoter region of Sfrp4 gene in the foetal brain, accounting for its decreased expression and contributing to the activation of the Wnt‐Catenin signalling. The study of PC12 cells treated with 5‐aza further approved that decreased methylation could result in the higher Sfrp4 expression. In the offspring hippocampus, protein levels of Hif1a and mRNA expression of Sfrp4 were unchanged, whereas Wnt signal pathway was inhibited. The data demonstrated that PH activated the Wnt pathway in the foetal brain, related to the hyper‐methylation of Sfrp4 as well as Hif1a signalling. Activated Wnt signalling might play acute protective roles to the foetal brain in response to hypoxia, also would result in disadvantageous influence on the offspring in long‐term.
LncRNA MALAT1/miR‐129 axis promotes glioma tumorigenesis by targeting SOX2
Authors: Xiong Zhiyong , Wang Luyang , Wang Qiangping , Yuan Ye
Published: May 2018

We aimed to explore the interaction among lncRNA MALAT1, miR‐129 and SOX2. Besides, we would investigate the effect of MALAT1 on the proliferation of glioma stem cells and glioma tumorigenesis. Differentially expressed lncRNAs in glioma cells and glioma stem cells were screened out with microarray analysis. The targeting relationship between miR‐129 and MALAT1 or SOX2 was validated by dual‐luciferase reporter assay. The expressions of MALAT1, miR‐129 and SOX2mRNA in both glioma non‐stem cells and glioma stem cells were examined by qRT‐PCR assay. The impact of MALAT1 and miR‐129 on glioma stem cell proliferation was observed by CCK‐8 assay, EdU assay and sphere formation assay. The protein expression of SOX2 was determined by western blot. The effects of MALAT1 and miR‐129 on glioma tumour growth were further confirmed using xenograft mouse model. The mRNA expression of MALAT1 was significantly up‐regulated in glioma stem cells compared with non‐stem cells, while miR‐129 was significantly down‐regulated in glioma stem cells. MALAT1 knockdown inhibited glioma stem cell proliferation via miR‐129 enhancement. Meanwhile, miR‐129 directly targeted at SOX2 and suppressed cell viability and proliferation of glioma stem cells by suppressing SOX2 expression. The down‐regulation of MALAT1 and miR‐129 overexpression both suppressed glioma tumour growth via SOX2 expression promotion in vivo. MALAT1 enhanced glioma stem cell viability and proliferation abilities and promoted glioma tumorigenesis through suppressing miR‐129 and facilitating SOX2 expressions.
Spondias mombin supplementation attenuated cardiac remodelling process induced by tobacco smoke
Authors: Lourenço Maria Angélica Martins , Braz Mariana Gobbo , Aun Aline Garcia , Pereira Bruna Letícia Buzati , Figueiredo Amanda Menezes , da Silva Renata Aparecida Cândido , Kazmarek Elisa Moya , Alegre Patrícia Helena Correa , Bachiega Tatiana Fernanda , Zanati Silmeia Garcia , Azevedo Paula Schmidt , Polegato Bertha Furlan , Fernandes Ana Angélica Henrique , de Paiva Sergio Alberto Rupp , Zornoff Leonardo Antonio Mamede , Minicucci Marcos Ferreira
Published: May 2018

The objective of this study was to investigate the influence of Spondias mombin (SM) supplementation on the cardiac remodelling process induced by exposure to tobacco smoke (ETS) in rats. Male Wistar rats were divided into 4 groups: group C (control, n = 20) comprised animals not exposed to cigarette smoke and received standard chow; group ETS (n = 20) comprised animals exposed to cigarette smoke and received standard chow; group ETS100 (n = 20) received standard chow supplemented with 100 mg/kg body weight/d of SM; and group ETS250 (n = 20) received standard chow supplemented with 250 mg/kg body weight/d of SM. The observation period was 2 months. The ETS animals had higher values of left cardiac chamber diameters and of left ventricular mass index. SM supplementation attenuated these changes. In addition, the myocyte cross‐sectional area (CSA) was lower in group C compared with the ETS groups; however, the ETS250 group had lower values of CSA compared with the ETS group. The ETS group also showed higher cardiac levels of lipid hydroperoxide (LH) compared with group C; and, groups ETS100 and ETS250 had lower concentrations of LH compared with the ETS group. Regarding energy metabolism, SM supplementation decreased glycolysis and increased the β‐oxidation and the oxidative phosphorylation. There were no differences in the expression of Nrf‐2, SIRT‐1, NF‐κB, interferon‐gamma and interleukin 10. In conclusion, our results suggest that ETS induced the cardiac remodelling process. In addition, SM supplementation attenuated this process, along with oxidative stress reduction and energy metabolism modulation.
How Robust Are Popular Models of Nominal Frictions?
Authors: KEEN BENJAMIN D. , KOENIG EVAN F.
Published: May 2018

We consider alternative combinations of nominal price and wage frictions in dynamic stochastic general equilibrium models fit to U.S. data. Since inflation was unanchored in the 1970s, we divide the data into early, middle, and late samples (1955–68, 1969–79, and 1983–2007, respectively). We find that prices are reoptimized more frequently and exhibit greater indexation to past inflation in the middle sample than in the other two samples, while wages are reoptimized with increasing frequency and display less evidence of indexation over time. Differences in price and wage setting across samples have important implications for the economy's response to key shocks.