bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Three Sludge Products from Co-Treatment of Wastewater on the Soil Properties and Plant Growth of Silty Loam
Authors: Ma Degang , Wang Yuxin , Ye Yu , Ge Xiaomei , Lu Xuebin
Published: April 2022

Currently, little is known about systematic comparisons of sludge products obtained from different sludge treatment processes in terms of land use. Moreover, it is worth evaluating whether the sludge produced from the co-treatment of industrial wastewater and domestic sewage can be applied to land use. In this study, three sludge products derived from the same municipal sludge—sludge biochar (SSB), dried sludge (DSS), and sludge compost (SSC)—were added to silty loam (SL) at a 20% mass ratio to assess their effects on soil structure, properties, and fertility. Chinese cabbage was planted as a model crop and its growth and physiological state were monitored. The experimental results showed that the water retention of the soil was significantly related to its porosity, and the moisture in the three sludge products-modified soil mainly existed in the form of free water. The addition of three sludge products increased the total porosity of SL. SSC enhanced the water retention of SL by increasing the capillary porosity, and SSB improved the gas permeability of SL by increasing the non-capillary porosity. The three sludge products all increased the content of large particles in the soil and improved the stability of the aggregates of SL. Among them, SSB and DSS had significant effects on improving the stability of the aggregates. Although the addition of the three sludge products improved the fertility of SL, compared with that of DSS and SSC, the addition of SSB made the growth indices of Chinese cabbage the best, indicating that SSB can effectively maintain soil nutrients. The heavy metal test results of Ni showed that SSB had a good stabilizing effect on heavy metals. Therefore, compared with drying and composting, pyrolysis of municipal sludge is more suitable for SL improvement.
Does Cyberostracism Reduce Prosocial Behaviors? The Protective Role of Psychological Resilience
Authors: Shi Linyu , Li Hao , Huang Lianqiong , Hou Yubo , Song Lili
Published: April 2022

To reduce the negative consequences of cyberostracism on prosocial behaviors, we developed a coping strategy based on psychological resilience, and revealed its effectiveness in combating the adverse effects of cyberostracism on prosocial behavior through two studies. Study 1 demonstrated that psychological resilience could mitigate the negative impact of cyberostracism on prosocial behaviors through experimental manipulation. By targeting continuously ostracized people with low resilience for an online self-help resilience intervention program, Study 2 confirmed that psychological resilience was effective in alleviating the detrimental effects of cyberostracism. These studies not only help us to recognize the negative effects of cyberostracism, but also extend Williams’ temporal need–threat model of ostracism in the context of online ostracism. As emerging technologies represent a promising new approach to intervention delivery, the most valuable contribution of this study is that we developed an online self-help psychological resilience intervention program that showed encouraging therapeutic effects and advantages for assisting in caring for a larger population of people who are at elevated risk for being cyberostracized.
Unhealthy Lifestyle Behaviours and Psychological Distress: A Longitudinal Study of Australian Adults Aged 45 Years and Older
Authors: George Emma S. , Davidson Ian , El Masri Aymen , Meade Tanya , Kolt Gregory S.
Published: April 2022

Individual associations between lifestyle behaviours and mental health have been established; however, evidence on the clustering of these behaviours and the subsequent impact on mental health is limited. The purpose of this study was to examine cross-sectional and longitudinal associations between combined unhealthy lifestyle behaviours (physical activity, sitting time, sleep duration, processed meat consumption, vegetable consumption, fruit consumption, smoking status, alcohol consumption) and the development of psychological distress (measured using the Kessler Psychological Distress Scale) in a large Australian sample. Participants were 163,707 Australian adults from the 45 and Up Study. Data from baseline (2006–2009) and follow-up wave 1 (2012) were analysed using binary logistic regression. The odds of reporting high or very high psychological distress at follow-up were significantly higher for those reporting five (AOR = 2.36; 95% CI 1.41–3.97, p = 0.001) or six or more (AOR = 3.04; 95% CI 1.62–5.69, p = 0.001) unhealthy lifestyle behaviours, in comparison to those reporting no unhealthy lifestyle behaviours at baseline. These findings suggest that a holistic, multi-faceted lifestyle approach addressing multiple behaviours may be required to support and promote positive mental health and to reduce the likelihood of psychological distress.
Depression, Insomnia, and Probable Post-Traumatic Stress Disorder among Survivors of the 2016 Kumamoto Earthquake and Related Factors during the Recovery Period Amidst the COVID-19 Pandemic
Authors: Ide-Okochi Ayako , Samiso Tomonori , Kanamori Yumie , He Mu , Sakaguchi Mika , Fujimura Kazumi
Published: April 2022

The aftereffects of the severe 2016 Kumamoto earthquake were complicated by the COVID-19 pandemic. This study aimed to identify mental health problems and related factors among survivors five years after the earthquake and clarify its long-term effects. A cross-sectional survey was conducted in 2020 among 19,212 survivors affected by the earthquake who moved from temporary to permanent housing. We analysed 8966 respondents (5135 women, 3831 men; mean age 62.25 ± 17.29 years). Logistic regression analysis was conducted to examine associations between mental health problems and socioeconomic factors. Prevalence rates of psychological distress, insomnia, and probable post-traumatic stress disorder were 11.9%, 35.2%, and 4.1%, respectively. Female gender (OR = 1.33, 95% CI = 1.13–1.57; OR = 1.21, 95% CI = 1.08–1.34; OR = 1.81, 95% CI = 1.41–2.32), public housing (OR = 2.14, 95% CI = 1.63–2.83; OR = 1.54, 95% CI = 1.26–1.88; OR = 2.41, 95% CI = 1.62–3.58), loneliness (OR = 9.08, 95% CI = 7.71–10.70; OR = 5.55, 95% CI = 4.90–6.30; OR = 3.52, 95% CI = 2.77–4.49), COVID-19-induced activity reduction (OR = 1.41, 95% CI = 1.19–1.66; OR = 1.86, 95% CI = 1.68–2.07; OR = 1.80, 95% CI = 1.40–2.31), and COVID-19-induced income reduction (OR = 1.33, 95% CI = 1.12–1.57; OR = 1.43, 95% CI = 1.28–1.59; OR = 1.92, 95% CI = 1.51–2.43) were significantly associated with mental health problems. These results suggest that gender, current housing, loneliness, and COVID-19 affected the survivors’ mental health during recovery.
Comparative Study of the SEM Evaluation, EDX Assessment, Morphometric Analysis, and Cyclic Fatigue Resistance of Three Novel Brands of NiTi Alloy Endodontic Files
Authors: Faus-Matoses Vicente , Pérez García Raúl , Faus-Llácer Vicente , Faus-Matoses Ignacio , Alonso Ezpeleta Óscar , Albaladejo Martínez Alberto , Zubizarreta-Macho Álvaro
Published: April 2022

In this study, we compare and analyze the scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), morphometry and cyclic fatigue resistance of Endogal, PathMax, and Smarttrack novel brands of nickel–titanium (NiTi) alloy endodontic files. Material and Methods: Thirty sterile NiTi endodontic rotary files were randomly selected and assigned to one of the following study groups: A: 25.08 F2 Endogal (EDG) (n = 10); B: 25.08 F2 Path Max Pro (PMP) (n = 10); and C: 25.06 Smarttrack (ST) (n = 10). Dynamic cyclic fatigue tests were conducted using a cyclic fatigue device in stainless steel artificial root canal systems with an apical diameter of 250 µm, curvature angle of 60°, radius of curvature of 3 mm, and taper of 6%. Additionally, we analyzed the NiTi endodontic rotary files using EDX, SEM, and morphometry after micro-CT scanning. The results were analyzed using Weibull statistical analysis and ANOVA testing. Results: SEM, EDX, and morphometric analyses showed differences between the three novel brands of NiTi endodontic rotary files. Moreover, statistically significant differences were observed between the number of cycles to failure and time to failure of the three novel brands of NiTi endodontic rotary files (p < 0.001). Conclusions: Smarttrack NiTi alloy endodontic reciprocating files display greater resistance to cyclic fatigue than Endogal and Path Max Pro NiTi alloy endodontic rotary files, due to the reciprocating movement and metallurgical composition.
Sequence Requirements for miR-424-5p Regulating and Function in Cancers
Authors: Xuan Jiangying , Liu Yingxia , Zeng Xiaoping , Wang Hongmei
Published: April 2022

MiRNAs (microRNAs) are the most abundant family of small noncoding RNAs in mammalian cells. Increasing evidence shows that miRNAs are crucial regulators of individual development and cell homeostasis by controlling various biological processes. Therefore, miRNA dysfunction can lead to human diseases, especially in cancers with high morbidity and mortality worldwide. MiRNAs play different roles in these processes. In recent years, studies have found that miR-424-5p is closely related to the occurrence, development, prognosis and treatment of tumors. This review discusses how miR-424-5p plays a role in different kinds of cancers from different stages of tumors, including its roles in (i) promoting or inhibiting tumorigenesis, (ii) regulating tumor development in the tumor microenvironment and (iii) participating in cancer chemotherapy. This review provides a deep discussion of the latest findings on miR-424-5p and its importance in cancer, as well as a mechanistic analysis of the role of miR-424-5p in various tissues through target gene verification and pathway analysis.
In Mitosis You Are Not: The NIMA Family of Kinases in Aspergillus, Yeast, and Mammals
Authors: Bachus Scott , Graves Drayson , Fulham Lauren , Akkerman Nikolas , Stephanson Caelan , Shieh Jessica , Pelka Peter
Published: April 2022

The Never in mitosis gene A (NIMA) family of serine/threonine kinases is a diverse group of protein kinases implicated in a wide variety of cellular processes, including cilia regulation, microtubule dynamics, mitotic processes, cell growth, and DNA damage response. The founding member of this family was initially identified in Aspergillus and was found to play important roles in mitosis and cell division. The yeast family has one member each, Fin1p in fission yeast and Kin3p in budding yeast, also with functions in mitotic processes, but, overall, these are poorly studied kinases. The mammalian family, the main focus of this review, consists of 11 members named Nek1 to Nek11. With the exception of a few members, the functions of the mammalian Neks are poorly understood but appear to be quite diverse. Like the prototypical NIMA, many members appear to play important roles in mitosis and meiosis, but their functions in the cell go well beyond these well-established activities. In this review, we explore the roles of fungal and mammalian NIMA kinases and highlight the most recent findings in the field.
Cloning and Organelle Expression of Bamboo Mitochondrial Complex I Subunits Nad1, Nad2, Nad4, and Nad5 in the Yeast Saccharomyces cerevisiae
Authors: Tsai Hsieh-Chin , Hsieh Cheng-Hung , Hsu Ching-Wen , Hsu Yau-Heiu , Chien Lee-Feng
Published: April 2022

Mitochondrial respiratory complex I catalyzes electron transfer from NADH to ubiquinone and pumps protons from the matrix into the intermembrane space. In particular, the complex I subunits Nad1, Nad2, Nad4, and Nad5, which are encoded by the nad1, nad2, nad4, and nad5 genes, reside at the mitochondrial inner membrane and possibly function as proton (H+) and ion translocators. To understand the individual functional roles of the Nad1, Nad2, Nad4, and Nad5 subunits in bamboo, each cDNA of these four genes was cloned into the pYES2 vector and expressed in the mitochondria of the yeast Saccharomyces cerevisiae. The mitochondrial targeting peptide mt gene (encoding MT) and the egfp marker gene (encoding enhanced green fluorescent protein, EGFP) were fused at the 5′-terminal and 3′-terminal ends, respectively. The constructed plasmids were then transformed into yeast. RNA transcripts and fusion protein expression were observed in the yeast transformants. Mitochondrial localizations of the MT-Nad1-EGFP, MT-Nad2-EGFP, MT-Nad4-EGFP, and MT-Nad5-EGFP fusion proteins were confirmed by fluorescence microscopy. The ectopically expressed bamboo subunits Nad1, Nad2, Nad4, and Nad5 may function in ion translocation, which was confirmed by growth phenotype assays with the addition of different concentrations of K+, Na+, or H+.
Physical Forces in Glioblastoma Migration: A Systematic Review
Authors: Grossen Audrey , Smith Kyle , Coulibaly Nangorgo , Arbuckle Benjamin , Evans Alexander , Wilhelm Stefan , Jones Kenneth , Dunn Ian , Towner Rheal , Wu Dee , Kim Young-Tae , Battiste James
Published: April 2022

The invasive capabilities of glioblastoma (GBM) define the cancer’s aggressiveness, treatment resistance, and overall mortality. The tumor microenvironment influences the molecular behavior of cells, both epigenetically and genetically. Current forces being studied include properties of the extracellular matrix (ECM), such as stiffness and “sensing” capabilities. There is currently limited data on the physical forces in GBM—both relating to how they influence their environment and how their environment influences them. This review outlines the advances that have been made in the field. It is our hope that further investigation of the physical forces involved in GBM will highlight new therapeutic options and increase patient survival. A search of the PubMed database was conducted through to 23 March 2022 with the following search terms: (glioblastoma) AND (physical forces OR pressure OR shear forces OR compression OR tension OR torsion) AND (migration OR invasion). Our review yielded 11 external/applied/mechanical forces and 2 tumor microenvironment (TME) forces that affect the ability of GBM to locally migrate and invade. Both external forces and forces within the tumor microenvironment have been implicated in GBM migration, invasion, and treatment resistance. We endorse further research in this area to target the physical forces affecting the migration and invasion of GBM.
The Growth, Lipid Accumulation and Fatty Acid Profile Analysis by Abscisic Acid and Indol-3-Acetic Acid Induced in Chlorella sp. FACHB-8
Authors: Lin Yihua , Dai Yue , Xu Weinan , Wu Xiaobin , Li Yanyan , Zhu Hongmei , Zhou Hantao
Published: April 2022

Microalgae are considered a promising source for biodiesel. The addition of plant hormone can exert a significant impact on the production of microalgae biomass and lipid accumulation. Nevertheless, the response of microalgae cells to hormones is species- or strain-dependent. It remains controversial which genes involved in strong increase of fatty acids production in response to abscisic acid (ABA) in Chlorella sp. FACHB-8 strain. We investigated cell growth, lipid accumulation, and fatty acid composition when ABA and indol-3-acetic acid (IAA) were used in the growth medium of Chlorella sp. FACHB-8. The four treatments, including 5 mg/L IAA (E1), 10 mg/L IAA (E2), 10 mg/L ABA (E3), the combination of 5 mg/L IAA and 5 mg/L ABA (E4), were found to increase cell growth, but only 10 mg/L ABA treatment could enhance the lipid accumulation. The fatty acid profile was changed by the addition of ABA, making fatty acids afflux from polyunsaturated fatty acids to monounsaturated and saturated fatty acids, which were suitable for diesel application. Furthermore, a transcriptome analysis was conducted, unraveling the differentially expressed genes enriched in fatty acid biosynthesis, fatty acid metabolism, and biosynthesis of the unsaturated fatty acid pathway in response to ABA. Our results clarified the correlation of fatty acid synthesis-related genes and fatty acid profiles, helping understand the potential response mechanism of Chlorella sp. FACHB-8 strain respond to ABA treatment.
Circ003429 Regulates Unsaturated Fatty Acid Synthesis in the Dairy Goat Mammary Gland by Interacting with miR-199a-3p, Targeting the YAP1 Gene
Authors: Jiao Peixin , Zhang Meimei , Wang Ziwei , Liang Gege , Xie Xiaolai , Zhang Yonggen , Chen Zhi , Jiang Qianming , Loor Juan J.
Published: April 2022

Fatty acid composition is a key factor affecting the flavor and quality of goat milk. CircRNAs are now recognized as important regulators of transcription, and they play an important role in the control of fatty acid synthesis. Thus, understanding the regulatory mechanisms controlling this process in ruminant mammary glands is of great significance. In the present study, mammary tissue from dairy goats during early lactation and the dry period (nonlactating) were collected and used for high-throughput sequencing. Compared to levels during the dry period, the expression level of circ003429 during early lactation was lower (12.68-fold downregulated). In isolated goat mammary epithelial cells, circ003429 inhibited the synthesis of triglycerides (TAG) and decreased the content of unsaturated fatty acids (C16:1, C18:1, and C18:2), indicating that this circRNA plays an important role in regulating lipid synthesis. A binding site for miR-199a-3p in the circ003429 sequence was detected, and a dual-luciferase reporter system revealed that circ003429 targets miR-199a-3p. Overexpression of circ003429 (pcDNA-circ003429) downregulated the abundance of miR-199a-3p. In contrast, overexpression of miR-199a-3p increased TAG content and decreased mRNA abundance of Yes-associated protein 1 (YAP1) (a target gene of miR-199a-3p), and TAG content was decreased and mRNA abundance was increased in response to overexpression of circ003429. These results indicate that circ003429 alleviates the inhibitory effect of miR-199a-3p on the mRNA abundance of YAP1 by binding miR-199a-3p, resulting in subsequent regulation of the synthesis of TAG and unsaturated fatty acids.
Emotional Processing Profile in Patients with First Episode Schizophrenia: The Influence of Neurocognition
Authors: Romero-Ferreiro Verónica , García-Fernández Lorena , Aparicio Ana Isabel , Martínez-Gras Isabel , Dompablo Mónica , Sánchez-Pastor Luis , Rentero David , Alvarez-Mon Miguel Ángel , Espejo-Saavedra Juan Manuel , Lahera Guillermo , Marí-Beffa Paloma , Santos José Luis , Rodriguez-Jimenez Roberto
Published: April 2022

This study sought to investigate the influence of neurocognition on the emotional processing profiles of patients with first-episode schizophrenia, using the 4-branch Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT) (Perceiving Emotions; Facilitating Emotions; Understanding Emotions and Managing Emotions). A sample of 78 patients with first-episode schizophrenia and a group of 90 non-psychiatric control subjects were included in this work. The initial results showed that patients had lower scores than controls for the “Understanding Emotions” and “Managing Emotions” MSCEIT branches. However, after controlling for neurocognition, the only deficits were found on the “Managing Emotions” branch of the MSCEIT. This branch can be considered as measuring a more sophisticated level of emotional processing, which may constitute a deficit in itself. In conclusion, patients with first-episode schizophrenia present deficits in social cognition at the highest level that seem to be independent from neurocognition. These findings support the inclusion of the “Managing Emotions” branch of the MSCEIT as part of the MCCB.
Gestational Diabetes Mellitus in Pregnant Women with Beta-Thalassemia Minor: A Matched Case-Control Study
Authors: Falcone Veronica , Heinzl Florian , Itariu Bianca Karla , Reischer Theresa , Springer Stephanie , Muin Dana Anaïs , Pateisky Petra , Foessleitner Philipp , Ott Johannes , Farr Alex , Rosta Klara
Published: April 2022

Pregnancy in women with thalassemia minor is considered safe. However, a higher incidence of maternal and neonatal complications in women with the disorder has been reported in the literature. This study aimed to determine whether there is an increased risk of gestational diabetes mellitus (GDM) in pregnant women with beta-thalassemia minor. We conducted a retrospective matched case-control study of 230 pregnant women who delivered at the Department of Obstetrics and Feto-Maternal Medicine at the Medical University of Vienna between the years 2008 and 2020, whereof 115 women had beta-thalassemia minor. We found no significant difference in the occurrence of GDM between the case group and control group of age and BMI-matched healthy women. However, we observed a significantly lower hemoglobin (Hb) and hematocrit (Ht) level during the first, the second, and the third trimesters of pregnancy, and postpartum (all: p < 0.001) among women with beta-thalassemia minor compared to the healthy controls. Neonates of women with beta-thalassemia were more likely to experience post-natal jaundice and excessive weight loss (p < 0.001). We conclude that GDM is not more likely to occur in pregnant women with beta-thalassemia minor. However, clinicians should be made aware of the risk of adverse maternal and neonatal outcomes. Furthermore, women with beta-thalassemia minor should undergo regular laboratory screening and multidisciplinary pregnancy care.
Intraocular Temperature Distribution in Eyes Undergoing Different Types of Surgical Procedures during Vitreous Surgery
Authors: Shinoda Kei , Matsumoto Soiti C. , Yagura Kazuma , Terauchi Gaku , Shoji Takuhei , Yoshikawa Yuji , Igawa Yuro , Mizota Atsushi , Miyake Yozo
Published: April 2022

Vitreous temperature has been reported to vary during intraocular surgery. We measured the temperature at three intraocular sites, just posterior to the crystalline lens (BL), mid-vitreous (MV), and just anterior to the optic disc (OD), and investigated temperature changes before and after different types of surgical procedures in 78 eyes. The mean temperature at the beginning was 30.1 ± 1.70 °C in the anterior chamber, 32.4 ± 1.41 °C at the BL, 33.8 ± 0.95 °C at the MV, and 34.7 ± 0.95 °C at the OD. It was lowest at the BL, and highest at the OD. The mean temperature after cataract surgery was slightly lower especially at an anterior location. Thus, the temperature gradient became slightly flatter. The mean temperature after core vitrectomy was even lower at all sites and a gradient of the temperature was not present. The mean temperature after membrane peeling was significantly higher than that after core vitrectomy, and there was no gradient. The mean temperature after fluid/air exchange was lower at the BL and higher at the MV and at the OD. Thus, a gradient of higher temperatures at the OD appeared. The intraocular temperature distribution is different depending on the surgical procedure which can then change the temperature gradient. The temperature changes at the different intraocular sites and the gradients should be further investigated because they may affect the physiology of the retina and the recovery process.
Ambulatory Care in Adult Congenital Heart Disease—Time for Change?
Authors: Coats Louise , Chaudhry Bill
Published: April 2022

Background: The adult congenital heart disease (ACHD) population is growing in size and complexity. This study evaluates whether present ambulatory care adequately detects problems and considers costs. Methods: A UK single-centre study of clinic attendances amongst 100 ACHD patients (40.4 years, median ACHD AP class 2B) between 2014 and 2019 and the COVID-19 restrictions period (March 2020–July 2021). Results: Between 2014 and 2019, there were 575 appointments. Nonattendance was 10%; 15 patients recurrently nonattended. Eighty percent of appointments resulted in no decision other than continued review. Electrocardiograms and echocardiograms were frequent, but new findings were rare (5.1%, 4.0%). Decision-making was more common with the higher ACHD AP class and symptoms. Emergency admissions (n = 40) exceeded elective (n = 25), with over half following unremarkable clinic appointments. Distance travelled to the ACHD clinic was 14.9 km (1.6–265), resulting in 433–564 workdays lost. During COVID 19, there were 127 appointments (56% in-person, 41% telephone and 5% video). Decisions were made at 37% in-person and 19% virtual consultations. Nonattendance was 3.9%; there were eight emergency admissions. Conclusion: The main purpose of the ACHD clinic is surveillance. Presently, the clinic does not sufficiently predict or prevent emergency hospital admissions and is costly to patient and provider. COVID-19 has enforced different methods for delivering care that require further evaluation.
Clinical, Radiological, and Histopathological Characteristics of Periosteal Chondrosarcoma with a Focus on the Frequency of Medullary Invasion
Authors: Nakagawa Makoto , Endo Makoto , Susuki Yosuke , Yokoyama Nobuhiko , Maekawa Akira , Nabeshima Akira , Iida Keiichiro , Fujiwara Toshifumi , Setsu Nokitaka , Matsunobu Tomoya , Matsumoto Yoshihiro , Yokoyama Ryohei , Yamada Yuichi , Kohashi Kenichi , Yamamoto Hidetaka , Oda Yoshinao , Iwamoto Yukihide , Nakashima Yasuharu
Published: April 2022

Periosteal chondrosarcoma is an extremely rare malignant cartilage-forming tumour that originates from the periosteum and occurs on the surface of bone. Often, it is difficult to distinguish periosteal chondrosarcoma from other tumours, and reports in the literature are scarce. This study aims to investigate the characteristics of periosteal chondrosarcoma, focusing particularly on medullary invasion. Among 33 periosteal cartilaginous tumours, seven patients with pathologically proven periosteal chondrosarcoma were identified retrospectively. The average tumour size was 5.4 cm in the long axis; two tumours were smaller than 3.0 cm. Six tumours were resected with a wide margin, and the remaining tumour had a marginal margin. Histology revealed that six tumours (85.7%) had invaded the medullary cavity; three of these did not show invasion into the medullary cavity on MRI evaluation. Neither local recurrence nor metastasis was observed among these patients. The frequency of invasion of the medullary cavity was higher than that reported previously. The recommended treatment for periosteal chondrosarcoma is resection with an adequate margin. Therefore, surgeons should consider the possibility of medullary invasion when attempting to achieve a histologically negative margin, even if the tumour does not show invasion into the medullary cavity on MRI.
P62/SQSTM1 beyond Autophagy: Physiological Role and Therapeutic Applications in Laboratory and Domestic Animals
Authors: Sabbieti Maria Giovanna , Marchegiani Andrea , Sufianov Albert A. , Gabai Vladimir L. , Shneider Alexander , Agas Dimitrios
Published: April 2022

Inflammation is the preceding condition for the development of mild and severe pathological conditions, including various forms of osteopenia, cancer, metabolic syndromes, neurological disorders, atherosclerosis, cardiovascular, lung diseases, etc., in human and animals. The inflammatory status is induced by multifarious intracellular signaling cascades, where cytokines, chemokines, arachidonic acid metabolites, adhesion molecules, immune cells and other components foster a “slow burn” at a local or systemic level. Assuming that countering inflammation limits the development of inflammation-based diseases, a series of new side-effects-free therapies was assessed in experimental and domestic animals. Within the targets of the drug candidates for quenching inflammation, an archetypal autophagic gear, the p62/sqstm1 protein, has currently earned attention from researchers. Intracellular p62 has been recently coined as a multi-task tool associated with autophagy, bone remodeling, bone marrow integrity, cancer progression, and the maintenance of systemic homeostasis. Accordingly, p62 can act as an effective suppressor of inflamm-aging, reducing oxidative stress and proinflammatory signals. Such an operational schedule renders this protein an effective watchdog for degenerative diseases and cancer development in laboratory and pet animals. This review summarizes the current findings concerning p62 activities as a molecular hub for cell and tissues metabolism and in a variety of inflammatory diseases and other pathological conditions. It also specifically addresses the applications of exogenous p62 (DNA plasmid) as an anti-inflammatory and homeostatic regulator in the treatment of osteoporosis, metabolic syndrome, age-related macular degeneration and cancer in animals, and the possible application of p62 plasmid in other inflammation-associated diseases.
Superstructure Detection in Nucleosome Distribution Shows Common Pattern within a Chromosome and within the Genome
Authors: Mishra Sujeet Kumar , Li Kunhe , Brauburger Simon , Bhattacherjee Arnab , Oiwa Nestor Norio , Heermann Dieter W.
Published: April 2022

Nucleosome positioning plays an important role in crucial biological processes such as replication, transcription, and gene regulation. It has been widely used to predict the genome’s function and chromatin organisation. So far, the studies of patterns in nucleosome positioning have been limited to transcription start sites, CTCFs binding sites, and some promoter and loci regions. The genome-wide organisational pattern remains unknown. We have developed a theoretical model to coarse-grain nucleosome positioning data in order to obtain patterns in their distribution. Using hierarchical clustering on the auto-correlation function of this coarse-grained nucleosome positioning data, a genome-wide clustering is obtained for Candida albicans. The clustering shows the existence beyond hetero- and eu-chromatin inside the chromosomes. These non-trivial clusterings correspond to different nucleosome distributions and gene densities governing differential gene expression patterns. Moreover, these distribution patterns inside the chromosome appeared to be conserved throughout the genome and within species. The pipeline of the coarse grain nucleosome positioning sequence to identify underlying genomic organisation used in our study is novel, and the classifications obtained are unique and consistent.
Dysregulation of the Amniotic PPARγ Pathway by Phthalates: Modulation of the Anti-Inflammatory Activity of PPARγ in Human Fetal Membranes
Authors: Antoine Audrey , De Sousa Do Outeiro Coraline , Charnay Coline , Belville Corinne , Henrioux Fanny , Gallot Denis , Blanchon Loïc , Minet-Quinard Régine , Sapin Vincent
Published: April 2022

Phthalates are reprotoxic pollutants that are omnipresent in the environment. Detectable in amniotic fluid, these compounds (with the most concentrated being mono-2-ethylhexyl phthalate (MEHP)) are in direct contact with fetal membranes (FMs). They can lead to the premature rupture of FMs by deregulating cellular and molecular pathways, such as, for example, the nuclear transcription factor peroxysome proliferator-activated receptor gamma (PPARγ) pathway. The objective was to study the impact of MEHP on the PPARγ pathway in FMs using amnion and choriodecidua across the three trimesters of pregnancy and the amniotic epithelial AV3 cell model by analyzing (i) PPARγ expression (mRNA and proteins) using RT-qPCR and Western blot assays; (ii) cytotoxicity and cell viability following MEHP treatment by lactate dehydrogenase (LDH) measurement and using Cell-counting Kit 8; and (iii) modulation by MEHP of PPARγ transcriptional activity (using a reporter gene assay) and PPARγ anti-inflammatory properties (by measuring IL6 and IL8 levels). PPARγ is expressed in the human amnion and choriodecidua during the three trimesters of pregnancy and in amniotic cells. In the AV3 cell line, MEHP is not cytotoxic and does not reduce cell viability, but it reduces PPARγ activity, here induced by a classical agonist without influencing its expression. MEHP also reduces PPARγ’s anti-inflammatory properties. In conclusion, PPARγ signaling is dysregulated by MEHP; this paves the way for future explorations to highlight the hypothesis of phthalates as an amniotic PPARγ disruptor that can explain the premature rupture of FMs.
Identifying Predictors of COVID-19 Mortality Using Machine Learning
Authors: Wan Tsz-Kin , Huang Rui-Xuan , Tulu Thomas Wetere , Liu Jun-Dong , Vodencarevic Asmir , Wong Chi-Wah , Chan Kei-Hang Katie
Published: April 2022

(1) Background: Coronavirus disease 2019 (COVID-19) is a dominant, rapidly spreading respiratory disease. However, the factors influencing COVID-19 mortality still have not been confirmed. The pathogenesis of COVID-19 is unknown, and relevant mortality predictors are lacking. This study aimed to investigate COVID-19 mortality in patients with pre-existing health conditions and to examine the association between COVID-19 mortality and other morbidities. (2) Methods: De-identified data from 113,882, including 14,877 COVID-19 patients, were collected from the UK Biobank. Different types of data, such as disease history and lifestyle factors, from the COVID-19 patients, were input into the following three machine learning models: Deep Neural Networks (DNN), Random Forest Classifier (RF), eXtreme Gradient Boosting classifier (XGB) and Support Vector Machine (SVM). The Area under the Curve (AUC) was used to measure the experiment result as a performance metric. (3) Results: Data from 14,876 COVID-19 patients were input into the machine learning model for risk-level mortality prediction, with the predicted risk level ranging from 0 to 1. Of the three models used in the experiment, the RF model achieved the best result, with an AUC value of 0.86 (95% CI 0.84–0.88). (4) Conclusions: A risk-level prediction model for COVID-19 mortality was developed. Age, lifestyle, illness, income, and family disease history were identified as important predictors of COVID-19 mortality. The identified factors were related to COVID-19 mortality.
Primary Intraosseous Osteolytic Meningioma with Aggressive Clinical Behaviour: Clinico-Pathologic Correlation and Proposed New Clinical Classification
Authors: Ahmed Nazmin , Ferini Gianluca , Haque Moududul , Umana Giuseppe Emmanuele , Scalia Gianluca , Chaurasia Bipin , Vats Atul , Rahman Asifur
Published: April 2022

(1) Introduction: Primary intraosseous osteolytic meningiomas (PIOM) are non-dural-based tumors predominantly presenting an osteolytic component with or without hyperostotic reactions. They are a subset of primary extradural meningiomas (PEM). In this study, we present a peculiar case with a systematic literature review and propose a new classification considering the limitations of previous classification systems. (2) Materials and Methods: Using a systematic search protocol in Google Scholar, PubMed, and Scopus databases, we extracted all case studies on PIOM published from inception to December 2020. A 46-year-old female patient form Dhaka, Bangladesh, was also described. The search protocol was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. (3) Results: Here, we present a 46-year-old female patient with PIOM who successfully underwent bifrontal craniotomy and gross total removal (GTR) of the tumor. At 6-month follow-up, no tumor recurrence was shown. Including our new case, 55 total cases from 47 articles were included in the analysis. PIOMs were in closer frequency among males (56.4%) and females (43.6%). The most common tumor location was the frontal and parietal calvarium, most commonly in the frontal bone (29.1%). Surgical resection was the predominant modality of treatment (87.3%); only 1.8% of patients were treated with radiotherapy, and 5.4% received a combination of surgery and radiotherapy. Gross total resection (GTR) was achieved in 80% of cases. Extracranial extension was reported in 41.8% of cases, dural invasion in 47.3%, and recurrence in 7.3%. Whole-body 68 Ga-DOTATOC PET/CT has also been reported as a useful tool both for differential diagnosis, radiotherapy contouring, and follow-up. Current treatments such as hydroxyurea and bevacizumab have variable success rates. We have also suggested a new classification which would provide a simple common ground for further research in this field. (4) Conclusions: Surgical resection, especially GTR, is the treatment of choice for PIOM, with a high GTR rate and low risk of complications and mortality. More research is needed on the differential diagnosis and specific treatment of PIOM.
Influence of Multiple Factors on the Workability and Early Strength Development of Alkali-Activated Fly Ash and Slag-Based Geopolymer-Stabilized Soil
Authors: Li Xinyu , Zhao Yufei , Hu Yong , Wang Guanci , Xia Minmin , Luo Biao , Luo Zhengdong
Published: April 2022

The complexity of composite geopolymer materials results in instability in the setting and hardening of geopolymer-stabilized soil. In order to determine the appropriate mix proportion scheme for composite geopolymer-stabilized soil, this study investigated the effects of two preparation methods, fly ash/slag ratio and alkali activator modulus, on workability and strength development trends in alkali-excited fly ash and slag-based geopolymer-stabilized soil. The results showed that the high ambient temperatures created by the one-step method were more conducive to the setting and hardening of the geopolymer-stabilized soil; its 3 d/28 d UCS (unconfined compression strength) ratio was 62.43–78.60%, and its 7 d/28 d UCS ratio was 70.37–83.63%. With increases of the alkali activator modulus or the proportion of fly ash, the setting time of stabilized soil was gradually prolonged, and its fluidity increased. Meanwhile, the strength development of stabilized soil was significantly affected by the proportion of fly ash and the alkali activator modulus; the maximum UCS value was obtained at II-2-O, prepared by the one-step method, with an alkali activator modulus of 1.2 and a fly ash/slag ratio of 20/80. Specifically, the 3, 7, and 28 d UCS values of II-2-O were 1.65, 1.89, and 2.26 MPa, respectively, and its 3 d/28 d UCS ratio and 7 d/28 d UCS ratio were 73.01% and 83.63%, respectively. These results will be of great importance in further research on (and construction guidance of) composite geopolymer-stabilized soil.
Microplastics in Wastewater by Washing Polyester Fabrics
Authors: Šaravanja Ana , Pušić Tanja , Dekanić Tihana
Published: April 2022

Microplastics have become one of the most serious environmental hazards today, raising fears that concentrations will continue to rise even further in the near future. Micro/nanoparticles are formed when plastic breaks down into tiny fragments due to mechanical or photochemical processes. Microplastics are everywhere, and they have a strong tendency to interact with the ecosystem, putting biogenic fauna and flora at risk. Polyester (PET) and polyamide (PA) are two of the most important synthetic fibres, accounting for about 60% of the total world fibre production. Synthetic fabrics are now widely used for clothing, carpets, and a variety of other products. During the manufacturing or cleaning process, synthetic textiles have the potential to release microplastics into the environment. The focus of this paper is to explore the main potential sources of microplastic pollution in the environment, providing an overview of washable polyester materials.
Microstructures and Mechanical Properties of H13 Tool Steel Fabricated by Selective Laser Melting
Authors: Lei Fei , Wen Tao , Yang Feipeng , Wang Jianying , Fu Junwei , Yang Hailin , Wang Jiong , Ruan Jianming , Ji Shouxun
Published: April 2022

H13 stool steel processed by selective laser melting (SLM) suffered from severe brittleness and scatter distribution of mechanical properties. We optimized the mechanical response of as-SLMed H13 by tailoring the optimisation of process parameters and established the correlation between microstructure and mechanical properties in this work. Microstructures were examined using XRD, SEM, EBSD and TEM. The results showed that the microstructures were predominantly featured by cellular structures and columnar grains, which consisted of lath martensite and retained austenite with numerous nanoscale carbides being distributed at and within sub-grain boundaries. The average size of cellular structure was ~500 nm and Cr and Mo element were enriched toward the cell wall of each cellular structure. The as-SLMed H13 offered the yield strength (YS) of 1468 MPa, the ultimate tensile strength (UTS) of 1837 MPa and the fracture strain of 8.48%. The excellent strength-ductility synergy can be attributed to the refined hierarchical microstructures with fine grains, the unique cellular structures and the presence of dislocations. In addition, the enrichment of solute elements along cellular walls and carbides at sub-grain boundaries improve the grain boundary strengthening.
Influence of the Grain Size Distribution of the Limestone Additives on the Color Properties and Phase Composition of Sintered Ceramic Materials Based on Cream-Firing Clays
Authors: Wiśniewska Kornelia , Kłosek-Wawrzyn Ewelina , Lach Radosław , Pichór Waldemar
Published: April 2022

The study focused on determining color changes in materials made of cream-firing clays from the Opoczno region (Poland) due to the addition of calcium carbonate in the form of limestone. Moreover, the influence of the grain size distribution of this additive on the color properties of the materials and their phase composition was determined. Test samples were prepared using theplastic method and fired at four different temperatures: 1120, 1140, 1160 and 1180 °C. The color properties of the surface of ceramic materials were determined in CIE-Lab color space using a colorimeter. Quantitative phase analysis was performed using the Rietveld method. The research showed that the addition of calcium carbonate causes an increase in the yellow color factor and a decrease in the red color factor and the brightness of the material. Moreover, it was proven that the grain size distribution of the additive used significantly influences the phase composition of the materials, thus determining the values of physical properties and the color of the materials.
Oxygen Vacancy Formation and Migration within the Antiphase Boundaries in Lanthanum Scandate-Based Oxides: Computational Study
Authors: Mastrikov Yuri A. , Gryaznov Denis , Sokolov Maksim N. , Zvejnieks Guntars , Popov Anatoli I. , Eglitis Roberts I. , Kotomin Eugene A. , Ananyev Maxim V.
Published: April 2022

The atomic structure of antiphase boundaries in Sr-doped lanthanum scandate (La1−xSrxScO3−δ) perovskite, promising as the proton conductor, was modelled by means of DFT method. Two structural types of interfaces formed by structural octahedral coupling were constructed: edge- and face-shared. The energetic stability of these two interfaces was investigated. The mechanisms of oxygen vacancy formation and migration in both types of interfaces were modelled. It was shown that both interfaces are structurally stable and facilitate oxygen ionic migration. Oxygen vacancy formation energy in interfaces is lower than that in the regular structure, which favours the oxygen vacancy segregation within such interfaces. The calculated energy profile suggests that both types of interfaces are advantageous for oxygen ion migration in the material.
Intrinsic Effect of Pulsed Current on the Recrystallization of Deformed AZ31 Alloy
Authors: Wu Jie , Wang Xiaobo
Published: April 2022

Two ensemble configurations were designed to investigate the intrinsic effect of a pulsed current on the recrystallization of rolled AZ31 alloy. The samples with a total reduction of about 60% were crystallized at 473K for 5 min when treated with the pulsed current. By forcing the pulsed current flow only through the graphite die, and the sample was heated by Joule effect, a microstructure with a grain size of ~5 μm was formed and the recrystallized fraction achieved 60% reduction. Moreover, a fully recrystallized microstructure with a grain size of ~9 μm was obtained when heated with Joule and athermal effects by forcing the pulsed current flow through the sample only. Based on the experimental results, the recrystallization behavior of deformed AZ31 under a pulsed current should be governed by the high Joule heating effect, which could generate transient high stress in the sample due to the nonsynchronous change in temperature and thermal expansion. The athermal effect of the pulsed current could enhance the dislocation mobility and thus accelerate coarsening of the recrystallization grains, but it should not be the key factor governing the recrystallization behavior of rolled AZ31B. This led to the p erroneous conclusion that the athermal effect of pulsed current played a crucial role in the recrystallization of deformed alloys.
Fracture Analysis of Ultrahigh-Strength Steel Based on Split Hopkinson Pressure Bar Test
Authors: Xiao Shihong , Luan Xiaosheng , Liang Zhiqiang , Wang Xibin , Zhou Tianfeng , Ding Yue
Published: April 2022

Impact loading is an important cause of fracture failure of ultrahigh-strength steel parts during service. Revealing the fracture mechanism of ultrahigh-strength steel under impact loading has important reference significance for the material preparation, part design, and manufacturing of such steel. Based on the split Hopkinson pressure bar (SHPB) test, the mechanical response characteristics of 45CrNiMoVA steel under impact loading were analyzed, and the true stress–true strain curves under a high strain rate (103 s−1) were obtained. It was found that under the simultaneous action of forward and tangential loading forces, a severe plastic deformation layer with a thickness of 20–30 μm was generated in the near impact-loading end face, which is the main cause for crack initiation and propagation. Under the condition of a high strain rate, the plastic flow stress of 45CrNiMoVA steel was characterized by the equilibrium of strain hardening and strain softening, and its impact fracture toughness decreased by 43.6%, resulting in increased quasi-cleavage fracture. Hence, severe surface plastic deformation during 45CrNiMoVA steel machining should be avoided, as it may lead to early failure.
A Neural Network-Based Model for Predicting Saybolt Color of Petroleum Products
Authors: Salehuddin Nurliana Farhana , Omar Madiah Binti , Ibrahim Rosdiazli , Bingi Kishore
Published: April 2022

Saybolt color is a standard measurement scale used to determine the quality of petroleum products and the appropriate refinement process. However, the current color measurement methods are mostly laboratory-based, thereby consuming much time and being costly. Hence, we designed an automated model based on an artificial neural network to predict Saybolt color. The network has been built with five input variables, density, kinematic viscosity, sulfur content, cetane index, and total acid number; and one output, i.e., Saybolt color. Two backpropagation algorithms with different transfer functions and neurons number were tested. Mean absolute error (MAE), root mean square error (RMSE), and coefficient of determination (R2) were used to assess the performance of the developed model. Additionally, the results of the ANN model are compared with the multiple linear regression (MLR). The results demonstrate that the ANN with the Levenberg–Marquart algorithm, tangent sigmoid transfer function, and three neurons achieved the highest performance (R2 = 0.995, MAE = 1.000, and RMSE = 1.658) in predicting the Saybolt color. The ANN model appeared to be superior to MLR (R2 = 0.830). Hence, this shows the potential of the ANN model as an effective method with which to predict Saybolt color in real time.
Challenges of Post-COVID-19 for a Sustainably Developed Society
Authors: Serpa Sandro , Ferreira Carlos Miguel
Published: April 2022

The COVID-19 pandemic has prompted both preventive and reactive disease control measures, such as lockdown, physical distancing, and mask use, among others [...]