bluuebunny/crossref_metadata_2025 huggingface dataset:
Ceramide Analog 5cc Overcomes TRAIL Resistance by Enhancing JNK Activation and Repressing XIAP Expression in Metastatic Colon Cancer Cells
Authors: Huang Qiqian , Liu Feiyan
Published: August 2023

Introduction: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered to be an effective apoptosis inducer due to its selectivity for tumor cells. However, many cancer cells, especially metastatic cancer cells, often exhibit resistance to TRAIL because their apoptotic pathway is impaired or their pro-survival pathway is overactivated. TRAIL resistance is the main obstacle to current TRAIL therapy. Nowadays, ceramide analogs represent a new class of potential anticancer agents. Therefore, we hypothesized that disrupting pro-survival signaling with ceramide analogs would increase TRAIL-mediated apoptosis. Methods: MTT assay and flow cytometry were conducted to evaluate the synergistic effect of ceramide analog 5cc on TRAIL in metastatic colon cancer cells. Western blot was used to detect signaling proteins affected by 5cc. RNA interference was performed to analyze the effects of specific gene on 5cc-enhanced apoptosis. Results: Ceramide analog 5cc markedly enhanced TRAIL-induced apoptosis evidenced by increased propidium iodide/annexin V double-positive cells and PARP cleavage in SW620 and LS411N cells. At the molecular level, 5cc significantly reduced the expression of anti-apoptotic protein X-linked inhibitor of apoptosis protein (XIAP) through the activation of the c-Jun n-terminal kinase (JNK) pathway which is critically involved in sensitizing tumor cells to TRAIL/5cc combination. JNK-silenced cells exhibited a significant reversal of TRAIL/5cc-mediated apoptosis. Conclusion: Our data demonstrated that ceramide analog 5cc overcomes TRAIL resistance by enhancing JNK activation and repressing XIAP expression in metastatic colon cancer cells.
Your Family Connects: A Theory-Based Intervention to Encourage Communication about Possible Inherited Cancer Risk among Ovarian Cancer Survivors and Close Relatives
Authors: Zhao Jingsong , McBride Colleen M. , Campbell Gavin P. , Pentz Rebecca D. , Escoffery Cam , Konomos Michael , Bellcross Cecelia , Ward Kevin , Shepperd James R. , Guan Yue
Published: August 2023

Introduction: Encouraging family communication about possible genetic risk has become among the most important avenues for achieving the full potential of genomic discovery for primary and secondary prevention. Yet, effective family-wide risk communication (i.e., conveying genetic risk status and its meaning for other family members) remains a critical gap in the field. We aim to describe the iterative process of developing a scalable population-based communication outreach intervention, Your Family Connects, to reach ovarian cancer survivors and close relatives to communicate the potential for inherited risk and to consider genetic counseling. Methods: Relational-level theories (e.g., interdependence theory) suggest that interventions to promote family cancer risk communication will be most effective if they consider the qualities of specific relationships and activate motives to preserve the relationship. Informed by these theories, we collaborated with 14 citizen scientists (survivors of ovarian cancer or relatives) and collected 261 surveys and 39 structured interviews over 12 weeks of citizen science activities in 2020. Results: The citizen science findings and consideration of relational-level theories informed the content and implementation of Your Family Connects (www.yourfamilyconnects.org). CS results showed survivors favor personal contact with close relatives, but relatives were open to alternative contact methods, such as through health professionals. Recognizing the need for varied approaches based on relationship dynamics, we implemented a relative contact menu to enable survivors identify at-risk relatives and provide multiple contact options (i.e., survivor contact, health professional contact, and delayed contact). In line with relational autonomy principles, we included pros and cons for each option, assisting survivors in choosing suitable contact methods for each relative. Discussion: Our developed intervention represents a novel application of relational-level theories and partnership with citizen scientists to expand genetic services reach to increase the likelihood for fair distribution of cancer genomic advances. The Your Family Connects intervention as part of a randomized trial in collaboration with the Georgia Cancer Registry compared with standard outreach.
BK Polyomavirus-Associated Urothelial Carcinoma of the Bladder with a Background of BK Polyomavirus Nephropathy in a Kidney Transplant Recipient
Authors: Iwasaki Sari , Takahashi Kenta , Katano Harutaka , Suzuki Tadaki , Sasaki Hajime , Harada Hiroshi , Takada Yusuke , Makita Keishi , Fukasawa Yuichiro , Tsuji Takahiro
Published: August 2023

Renal transplant recipients are at increased risk for the development of a malignant neoplasm. Polyomavirus-associated urothelial carcinoma is a rare tumor that occurs in renal transplant recipients, with approximately 41 cases reported since 2002. It accounts for 27–31% of all post-transplant urothelial carcinomas and develops at an average of 8.5 years after transplantation. Histologically, it shows high-grade urothelial carcinoma (95.1%) with a high frequency of glandular differentiation and micropapillary structures (58.5%) and positive immunohistochemistry for polyomavirus large T antigen, p53 (92.9%), and p16 (100%). We encountered a case of BK polyomavirus (BKPyV)-associated urothelial carcinoma of the bladder diagnosed 54 months after kidney transplantation. Histologically, it was a high-grade urothelial carcinoma with micropapillary features, and immunohistochemically, it was diffusely positive for polyomavirus large T antigen, p16, and p53. BKPyV DNA and mRNA for BKPyV large T antigen have been identified in tissues using real-time polymerase chain reaction. The same sequence of the BKPyV VP1 genome hypervariable region was detected in both transplanted kidney tissue with polyomavirus nephropathy and urothelial carcinoma tissue, suggesting that polyomavirus-associated urothelial carcinoma developed in a background of persistent polyomavirus nephropathy. This case showed typical histological features and was detected and treated at an earlier stage than has been reported. It is important to keep in mind that polyomavirus-associated urothelial carcinoma can develop early after transplantation and might be associated with polyomavirus nephropathy. Because of its rapidly progressive nature, careful follow-up with urine cytology and cystoscopy is necessary. We report this case with a literature review.
Association between Mobile Phone Use and Incidence of Dementia: A Prospective Cohort Study Using the UK Biobank
Authors: Zhao Houyu , Wen Qiaorui , Zhuo Lin , Wang Shengfeng , Zhan Siyan
Published: August 2023

Introduction: The rapid growth in mobile phone use has led to public concern about its potential effects on the risk of dementia. This study aimed to investigate the association between mobile phone use in daily life and the risk of dementia incidence in community-dwelling adults based on the data from the UK Biobank. Methods: Participants in the UK Biobank aged 60 years or older with no diagnosis of dementia at the time of recruitment were included in this prospective cohort study. A Cox regression model adjusted for sociodemographic characteristics, general health factors, mental health, lifestyle factors, comorbidities, and medication use was used to estimate the hazard ratio (HR) and confidence interval (CI) of the association between mobile phone use and dementia risk. Results: The final analyses included 213,181 participants. During a median follow-up period of 12.4 years, 6,344 cases of incident dementia occurred. Mobile phone use displayed a modest association with lower risk of dementia incidence, with HRs of 0.85 (95% CI: 0.79–0.91), 0.85 (95% CI: 0.80–0.91), 0.78 (95% CI: 0.71–0.86), 0.86 (95% CI: 0.77–0.96), and 0.83 (95% CI: 0.70–0.98) for participants who reported phone call usage of fewer than 5 min, 5–29 min, 30–59 min, 1–3 h, and more than 3 h per week, respectively, compared with nonusers. In addition, the proportions of the association medicated by family/friend visits and other leisure/social activities were 2.62% (95% CI: −0.64–6.51) and 2.22% (95% CI: 1.12–4.12), respectively. Conclusions: Daily mobile phone use is significantly associated with a reduced risk of incident dementia in community-dwelling adults in the UK Biobank population. This association seems to be mediated by improved social and mental activities.
Discovery and Potential Functional Characterization of Long Noncoding RNAs Associated with Familial Acne Inversa with NCSTN Mutation
Authors: He Yanyan , Wang Wenzhu , Ma Xiao , Duan Zhimin , Wang Baoxi , Li Min , Xu Haoxiang
Published: August 2023

Background: Long noncoding RNAs (lncRNAs) are associated with many dermatologic diseases. However, little is known about the regulatory function of lncRNAs in familial acne inversa (AI) patients with nicastrin (NCSTN) mutation. Objectives: The aim of this study was to explore the regulatory function of lncRNAs in familial AI patients with NCSTN mutation. Methods: The expression profiles of lncRNAs and mRNAs in skin tissues from familial AI patients with NCSTN mutation and healthy individuals were analysed in this study via RNA sequencing (RNA-seq). Results: In total, 359 lncRNAs and 1,863 mRNAs were differentially expressed between the two groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the dysregulated mRNAs targeted by lncRNAs were mainly associated with the immune regulation, Staphylococcus aureus infection and B cell receptor signalling pathways. The lncRNA-miRNA-mRNA coexpression network contained 265 network pairs comprising 55 dysregulated lncRNAs, 11 miRNAs, and 74 mRNAs. Conservation analysis of the differentially expressed lncRNAs between familial AI patients with NCSTN mutation and Ncstn keratinocyte-specific knockout (NcstnΔKC) mice identified 6 lncRNAs with sequence conservation; these lncRNAs may participate in apoptosis, proliferation, and skin barrier function. Conclusions: These findings provide a direction for exploring the regulatory mechanisms underlying the progression of familial AI patients with NCSTN mutation.
The Leucine-Rich α2-Glycoprotein-1 Levels in Patients with Multiple Myeloma
Authors: Kaçmaz Murat , Oğuzman Hamdi
Published: August 2023

Introduction: Angiogenesis is considered important in the pathogenesis of multiple myeloma (MM), as well as in the targeted treatment of the disease. Leucine-rich α2-glycoprotein 1 (LRG1) is a protein that participates in angiogenesis and its effect on solid organ tumors has been investigated recently. This study aimed to investigate the relationship between MM and LRG1. Methods: The MM patients who admitted to Hatay Mustafa Kemal University Hematology Clinic between September 2021 and October 2022 were included in the study. The study consists of a total of 4 groups: newly diagnosed MM (NDMM), relapsed refractory MM (RRMM), MM in remission (Rem-MM), and control group. Demographic data were retrieved from hospital records. Blood samples of our study groups were centrifuged at 1,500 × g for 10 min and serum was collected. LRG1, IL-6, IL-8, TGF-β1, HIF-1α, FGF-2, and VEGF levels were analyzed in all groups by ELISA method, and statistical analysis was performed. Results: A total of 112 individuals, including NDMM (n: 27), RRMM (n: 18), Rem-MM (n: 42), and control group (n: 25), were enrolled in the study. Based on the analyses, the NDMM group exhibited significantly elevated levels of LRG1 (p < 0.001), TGF-1 (p < 0.001), and HIF-1α (p = 0.046, p < 0.001, and p = 0.003 compared to the RRMM, Rem-MM, and control groups, respectively) compared to the other groups. LRG1 levels were positively correlated with creatinine (r: 0.363, p = 0.001), calcium (r: 0.344, p = 0.001), total protein (r: 0.473, p < 0.001), erythrocyte sedimentation rate (r: 0.547, p < 0.001), lactate dehydrogenase (r: 0.321, p = 0.003), beta-2-microglobulin (r: 0.312, p = 0.017), IL-6 (r: 0.478, p < 0.001), IL-8 (r: 0.240, p = 0.03), TGF-β1 (r: 0.521, p < 0.001), and HIF-1α (r: 0.321, p = 0.003) levels and were negatively correlated with hemoglobin (r: −0.512, p < 0.001) and albumin (r: −0.549, p < 0.001) levels. Receiver operating characteristics (ROC) analysis revealed the association of LRG1 with the highest AUC value of 0.959 (95% CI: 0.904–1, p < 0.001) and the optimal cut-off value of 534.95 ng/mL (sensitivity: 93% and specificity: 99%) in the NDMM group compared to the control group. Conclusion: In this study, providing data for the first time on LRG1 levels in the setting of MM. LRG1 levels were found to be significantly higher in NDMM patients and in our study discriminate this patient population from RRMM, Rem-MM, and normal controls. Therefore, LRG1 seems to a potential biomarker that should be evaluated in future studies addressing the diagnosis, staging, follow-up, prognosis, and treatment target of MM.
Long-Term Trajectories of Biomarkers, Functional, and Echocardiographic Parameters in Patients with Chronic Heart Failure from Dilated or Ischaemic Cardiomyopathy
Authors: Täger Tobias , Rößmann Paulina , Frey Norbert , Estler Bent , Mäck Mirjam , Schlegel Philipp , Beckendorf Jan , Frankenstein Lutz , Fröhlich Hanna
Published: August 2023

Introduction: The long-term evolution of clinical, echocardiographic, and laboratory parameters of cardiac function in patients with chronic heart failure (HF) with either reduced (HFrEF) or mildly reduced (HFmrEF) left ventricular ejection fraction (LVEF) is incompletely characterised. Methods: We identified patients with chronic stable HF who presented at least twice to a university HF outpatient clinic between 1995 and 2021. Trajectories of NYHA functional class, LVEF, left ventricular internal end-diastolic diameter (LVIDD), NT-proBNP concentrations, and HF treatment over 10 years of follow-up were analysed using fractional polynomials. Analyses were repeated after stratifying patients according to aetiology (ischaemic vs. dilated) or HF category (HFrEF vs. HFmrEF). Results: A total of 2,132 patients were included, of whom 51% had ischaemic and 49% had dilated HF. Eighty six percent and 14% were classified as HFrEF and HFmrEF, respectively. Mean LVEF was 28 ± 10%, and median NT-proBNP and estimated glomerular filtration rate values were 1,170 (385–3,176) pmol/L and 81 (62–100) mL/min/1.73 m2, respectively. Median follow-up was 5.2 (2.6–9.2) years. Overall, NYHA functional class and LVIDD trajectories were U-shaped, whereas LVEF and NT-proBNP concentrations markedly improved during the first year and remained stable thereafter. However, the evolution of HF parameters significantly differed with respect to HF category and aetiology, with greater improvements seen in patients with HFrEF of non-ischaemic origin. Improvements in HF variables were associated with optimization of HF therapy, notably with initiation and up-titration of renin-angiotensin-system blockers. Conclusion: This study provides insights into the natural history of HF in a large cohort of well-treated chronic HF outpatients with respect to subgroups of HF and different aetiologists.
Detection of Hybrid Fusion Transcripts, Aberrant Transcript Expression, and Specific Single Nucleotide Variants in Acute Leukemia and Myeloid Disorders with Recurrent Gene Rearrangements
Authors: Li Yuewei , Deng Kaifang , Kaner Justin , Geyer Julia T. , Ouseph Madhu , Fang Frank , Xu Kemin , Roboz Gail , Kluk Michael J.
Published: August 2023

Introduction: A variety of gene rearrangements and molecular alterations are key drivers in the pathobiology of acute leukemia and myeloid disorders; current classification systems increasingly incorporate these findings in diagnostic algorithms. Therefore, clinical laboratories require versatile tools, which can detect an increasing number and variety of molecular and cytogenetic alterations of clinical significance. Methods: We validated an RNA-based next-generation sequencing (NGS) assay that enables the detection of: (i) numerous hybrid fusion transcripts (including rare/novel gene partners), (ii) aberrantly expressed EVI1 (MECOM) and IKZF1 (Del exons 4–7) transcripts, and (iii) hotspot variants in KIT, ABL1, NPM1 (relevant in the context of gene rearrangement status). Results: For hybrid fusion transcripts, the assay showed 98–100% concordance for known positive and negative samples, with an analytical sensitivity (i.e., limit of detection) of approximately 0.8% cells. Samples with underlying EVI1 (MECOM) translocations demonstrated increased EVI1 (MECOM) expression. Aberrant IKZF1 (Del exons 4–7) transcripts detectable with the assay were also present on orthogonal reverse transcription PCR. Specific hotspot mutations in KIT, ABL1, and NPM1 detected with the assay showed 100% concordance with orthogonal testing. Lastly, several illustrative samples are included to highlight the assay’s clinically relevant contributions to patient workup. Conclusion: Through its ability to simultaneously detect various gene rearrangements, aberrantly expressed transcripts, and hotspot mutations, this RNA-based NGS assay is a valuable tool for clinical laboratories to supplement other molecular and cytogenetic methods used in the diagnostic workup and in clinical research for patients with acute leukemia and myeloid disorders.
The Glasgow Prognostic Score Predicts Survival Outcomes in Patients with Extensive-Stage Small Cell Lung Cancer
Authors: Igawa Satoshi , Yamamoto Hiroki , Yamada Kaori , Akazawa Yuki , Manaka Hiroya , Yagami Yuri , Nakahara Yoshiro , Sato Takashi , Mitsufuji Hisashi , Sasaki Jiichiro , Naoki Katsuhiko
Published: August 2023

Introduction: The Glasgow prognostic score (GPS) is an inflammation-related score based on C-reactive protein and albumin concentrations. Few studies have assessed the correlation between the GPS and the efficacy of chemotherapy in patients with extensive-stage small cell lung cancer (ES-SCLC). Therefore, this study aimed to evaluate the utility of the GPS in predicting the survival outcomes of patients with ES-SCLC. Methods: This retrospective study evaluated patients with ES-SCLC who had undergone chemotherapy between February 2008 and November 2021. GPS values were evaluated before the initiation of first-line chemotherapy. The Kaplan-Meier method and Cox proportional hazards models were used to assess progression-free survival (PFS) and overall survival (OS). Results: The GPS values of the 113 patients were zero (54 patients, 48%), 1 (37 patients, 33%), and 2 (22 patients, 19%). The median follow-up duration was 10.7 months. Median PFS was 6.2, 5.6, and 3.8 months in the GPS 0, 1, and 2 groups, respectively, suggesting that the GPS zero group had a significantly more favorable PFS than the GPS 2 group (p < 0.001). Median OS was 17.1, 9.4, and 5.6 months in the GPS 0, 1, and 2 groups, respectively, suggesting that the GPS zero group had a significantly more favorable OS than the GPS 2 group (p = 0.001). Multivariate analysis confirmed that a GPS of 2 independently predicted unfavorable PFS (hazard ratio [HR], 2.89; 95% confidence interval [CI]: 1.68–4.88; p < 0.001) and OS (HR, 3.49 [95% CI: 1.83–6.63], p < 0.001). Conclusion: The study’s findings suggest that the GPS can predict the survival outcomes of patients with ES-SCLC who have undergone chemotherapy. The GPS is an easy-to-calculate biomarker and would be ideal for routine use in clinical settings.
Application of the Cytokinesis-Block Micronucleus Assay for High-Dose Exposures Using Imaging Flow Cytometry
Authors: Beaton-Green Lindsay A. , Mayenburg Jessica M. , Marro Leonora , Hassan Eman M. , Cuadros Sanchez Sarita , Darwish Riham , Lachapelle Sylvie , Adam Nadine , Burtt Julie J. , Van Den Hanenberg Cyndi , Rodrigues Matthew A. , Wang Qi , Brenner David J. , Turner Helen C. , Wilkins Ruth C.
Published: August 2023

The cytokinesis-block micronucleus assay is a well-established method to assess radiation-induced genetic damage in human cells. This assay has been adapted to imaging flow cytometry (IFC), allowing automated analysis of many cells, and eliminating the need to create microscope slides. Furthermore, to improve the efficiency of assay performance, a small-volume method previously developed was employed. Irradiated human blood samples were cultured, stained, and analyzed by IFC to produce images of the cells. Samples were run using both manual and 96-well plate automated acquisition. Multiple parameter-based image features were collected for each sample, and the results were compared to confirm that these acquisition methods are functionally identical. This paper details the multi-parametric analysis developed and the resulting calibration curves up to 10 Gy. The calibration curves were created using a quadratic random coefficient model with Poisson errors, as well as a logistic discriminant function. The curves were then validated with blinded, irradiated samples, using relative bias and relative mean square error. Overall, the accuracy of the dose estimates was adequate for triage dosimetry (within 1 Gy of the true dose) over 90% of the time for lower doses and about half the time for higher doses, with the lowest success rate between 5 and 6 Gy where the calibration curve reached its peak and there was the smallest change in MN/BNC with dose. This work describes the application of a novel multi-parametric analysis that fits the calibration curves and allows dose estimates up to 10 Gy, which were previously limited to 4 Gy. Furthermore, it demonstrates that the results from samples acquired manually and with the autosampler are functionally similar.
CT-Based and CTA-Based Posterior Circulation ASPECTS in Acute Basilar Artery Occlusion: An Agreement Study
Authors: Zhang Feng , Huang Zhixin , Lu Wei , Wang Zi , Lu Yanan , Wang Meng , Zhang Pan , Hu Miaomiao , Xiao Lulu , Liu Xinfeng , Sun Wen
Published: August 2023

Background: Posterior circulation Acute Stroke Prognosis Early Computed Tomography Scores (pc-ASPECTS) is a rapid method of measuring early ischemic changes. However, there was no agreement study about pc-ASPECTS. Objectives: The purpose of the present study was to evaluate the agreement on pc-ASPECTS based on non-contrast CT (NCCT) and CT angiography (CTA) source images in patients with acute basilar artery occlusion (BAO). Methods: We prospectively enrolled consecutive patients with acute BAO from January 2022 to August 2022 at the First Affiliated Hospital of University of Science and Technology of China. The NCCT and CTA were scored independently by 15 raters during 2 different reading sessions at least 3 weeks apart. The pc-ASPECTS based on NCCT and CTA were analyzed on the full scale or were dichotomized (0–6 vs. 7–10, 0–7 vs. 8–10, and 0–8 vs. 9–10). The level of agreement was measured using Fleiss κ statistics. Results: The median (IQR) CT-based pc-ASPECTS was 8 (6.75–9). The interrater agreement for CT-based pc-ASPECTS (κ = 0.133 [0.132–0.133]) and CTA-based pc-ASPECTS (κ = 0.204 [0.203–0.204]) was slight for all raters. Dichotomizations obtaining the highest concordance for the CT-based pc-ASPECTS (0–6 vs. 7–10) and the CTA-based pc-ASPECTS (0–8 vs. 9–10) failed to increase the interrater agreement to a substantial level (κ = 0.350 [0.348–0.351] and 0.396 [0.395–0.398], respectively). Intrarater agreement for global CT-based pc-ASPECTS was less than substantial for the 14/15 raters and reached the level of substantial for the 3/15 raters with dichotomization. Conclusions: Agreement between clinicians assessing CT-based and CTA-based pc-ASPECTS cannot be sufficient to make reproducible clinical decisions and assessments. The dichotomization failed to improve interrater and intrarater agreement to the level of substantial.
Vagal Nerve Therapy in the Management of Obesity: A Systematic Review and Meta-Analysis
Authors: Fadel Michael G. , Fehervari Matyas , Das Bibek , Soleimani-Nouri Payam , Ashrafian Hutan
Published: August 2023

Introduction: The vagus nerve has an important role in satiety, metabolism, and autonomic control in upper gastrointestinal function. However, the role and effects of vagal nerve therapy on weight loss remain controversial. This systematic review and meta-analysis assessed the effects of vagal nerve therapy on weight loss, body mass index (BMI), and obesity-related conditions. Methods: MEDLINE, EMBASE, and CINAHL databases were searched for studies up to April 2022 that reported on percentage excess weight loss (%EWL) or BMI at 12 months or remission of obesity-related conditions following vagal nerve therapy from January 2000 to April 2022. Weighted mean difference (WMD) was calculated, meta-analysis was performed using random-effects models, and between-study heterogeneity was assessed. Results: Fifteen studies, of which nine were randomised controlled trials, of 1,447 patients were included. Vagal nerve therapy led to some improvement in %EWL (WMD 17.19%; 95% confidence interval [CI]: 10.94–23.44; p < 0.001) and BMI (WMD −2.24 kg/m2; 95% CI: −4.07 to −0.42; p = 0.016). There was a general improvement found in HbA1c following vagal nerve therapy when compared to no treatment given. No major complications were reported. Conclusions: Vagal nerve therapy can safely result in a mild-to-moderate improvement in weight loss. However, further clinical trials are required to confirm these results and investigate the possibility of the long-term benefit of vagal nerve therapy as a dual therapy combined with standard surgical bariatric interventions.
Eating With Bloom on the Sixteenth of June: Food in Ulysses
Authors: Öztarhan Esra
Published: August 2023

Food is not only a biological need but also a socio-cultural phenomenon. Though food is a vital need for people to survive, it does not only contain taste and ingredients, but contains other things like emotions, symbols of identity, power relations, gender roles, economy and social rules. Food choices affect lots of areas in the society and the life of individuals. This article will analyze the use of food in James Joyce’s Ulysses through its ordinary hero Leopold Bloom. The novel, as the epic of the body, uses food as a reflection of everyday life and grotesque realism. Moreover, food is used throughout the novel to exemplify Bloom’s personal and social identity. Bloom is a pacifist, nontraditionally masculine man, half Irish, half Jewish and also feels like an outsider in Dublin. All these aspects are narrated in Ulysses through the food he chooses to eat. Joyce has created a novel about life with all its aspects, including food.
Immediate Tooth Replacement in the Esthetic Zone with the Socket Shield Technique and a Novel Body-Shift Implant: A Pilot Study with Up to 3 Years of Follow-up
Authors: Gluckman Howard , Pontes Carla Cruvinel , Chu Stephen
Published: August 2023

Successful rehabilitation of the anterior maxilla remains a challenge, primarily due to postextraction ridge collapse, which can lead to gingival recession and a nonesthetic appearance. The socket shield technique presents a viable alternative for immediate dental implant placement in the esthetic zone. This pilot study aimed to evaluate the survival, complication rates, and marginal bone loss around body-shift implants placed in fresh extraction sockets with the socket shield technique to replace maxillary incisors. After socket shield preparation, 14 hopeless incisors were extracted and immediately replaced by a body-shift implant (Inverta, Southern Implants) at baseline. Patients were followed up for 12 to 39 months (mean: 18.1 ± 8.2 months). The average age was 52.3 ± 16.9 years, and the survival rate was 100%. One socket shield presented minimal internal exposure that was successfully treated with a connective tissue graft. The mean PES score was 12.9 ± 1.2. Mean interproximal marginal bone loss was –0.4 ± 0.5 mm. Body-shift implants can be a promising alternative for immediate tooth replacement combined with the socket shield technique. The superior esthetic outcomes remained stable, suggesting that the extra space created by the narrow cervical portion of the implant is beneficial for preserving the alveolar bone and limiting internal shield exposure.
A Surgical Approach to Root Coverage and Correction of Mucogingival Conditions and Deformities in Mandibular Incisors with Isolated Gingival Recession: Free Mucogingival Graft. A Pilot Prospective Cohort Study
Authors: Moreno Rodríguez Jose A. , Ortiz Ruiz Antonio J.
Published: August 2023

The aim of this study was to present a periodontal plastic surgery approach to treat gingival recessions (GRs) and correct mandibular incisor mucogingival conditions and deformities. Isolated deep GRs (≥ 3 mm) in the mandibular incisors (n = 24 teeth) were treated: 66.6% of sites were recession types 2 or 3, and 58.3% of teeth were malpositioned. Recessions were treated using free mucogingival grafts (FMGs) harvested from the buccal aspect of donor teeth with altered passive eruption or healthy periodontal support, with < 3 mm between the cementoenamel junction and the buccal alveolar crest. Clinical parameters (GR, clinical attachment level, interproximal papilla tip location, keratinized tissue, vestibule depth) and root coverage esthetic score were evaluated at 9 months. FMG significantly reduced GR (P < .001) and increased keratinized tissue (P < .001) without loss of vestibule depth (P > .05). Mean root coverage was 94.37% ± 10.60%, mean residual GR was 0.08 ± 0.65 mm, and the mean root coverage esthetic score was 8.9 ± 1.24. Recession types 2/3 showed significant interproximal clinical attachment gain (P < .05). The interproximal papilla was significantly augmented at sites with papilla loss (P < .001). No clinical attachment loss (P = .346) was detected at donor sites. These results suggest that FMG is a promising root coverage approach for recession types 1, 2, and 3, correcting mucogingival conditions and deformities and reconstructing the interproximal papilla.
Organ Donation After Out-of-Hospital Cardiac Arrest: A Scientific Statement From the International Liaison Committee on Resuscitation
Authors: Morrison Laurie J. , Sandroni Claudio , Grunau Brian , Parr Michael , Macneil Finlay , Perkins Gavin D. , Aibiki Mayuki , Censullo Eileen , Lin Steve , Neumar Robert W. , Brooks Steven C. ,   on behalf of the International Liaison Committee on Resuscitation
Published: August 2023

AIM OF THE REVIEW:

Improving rates of organ donation among patients with out-of-hospital cardiac arrest who do not survive is an opportunity to save countless lives. The objectives of this scientific statement were to do the following: define the opportunity for organ donation among patients with out-of-hospital cardiac arrest; identify challenges and opportunities associated with organ donation by patients with cardiac arrest; identify strategies, including a generic protocol for organ donation after cardiac arrest, to increase the rate and consistency of organ donation from this population; and provide rationale for including organ donation as a key clinical outcome for all future cardiac arrest clinical trials and registries.

METHODS:

The scope of this International Liaison Committee on Resuscitation scientific statement was approved by the International Liaison Committee on Resuscitation board and the American Heart Association, posted on
ILCOR.org
for public comment, and then assigned by section to primary and secondary authors. A unique literature search was completed and updated for each section.

RESULTS:

There are a number of defining pathways for patients with out-of-hospital cardiac arrest to become organ donors; however, modifications in the Maastricht classification system need to be made to correctly identify these donors and to report outcomes with consistency. Suggested modifications to the minimum data set for reporting cardiac arrests will increase reporting of organ donation as an important resuscitation outcome. There are a number of challenges with implementing uncontrolled donation after cardiac death protocols, and the greatest impediment is the lack of legislation in most countries to mandate organ donation as the default option. Extracorporeal cardiopulmonary resuscitation has the potential to increase organ donation rates, but more research is needed to derive neuroprognostication rules to guide clinical decision-making about when to stop extracorporeal cardiopulmonary resuscitation and to evaluate cost-effectiveness.

CONCLUSIONS:

All health systems should develop, implement, and evaluate protocols designed to optimize organ donation opportunities for patients who have an out-of-hospital cardiac arrest and failed attempts at resuscitation.
Sex Differences in Poststroke Cognitive Impairment: A Multicenter Study in 2343 Patients With Acute Ischemic Stroke
Authors: Exalto Lieza G. , Weaver Nick A. , Kuijf Hugo J. , Aben Hugo P. , Bae Hee-Joon , Best Jonathan G. , Bordet Régis , Chen Christopher P.L.H. , van der Giessen Ruben S. , Godefroy Olivier , Gyanwali Bibek , Hamilton Olivia K.L. , Hilal Saima , Huenges Wajer Irene M.C. , Kim Jonguk , Kappelle L. Jaap , Kim Beom Joon , Köhler Sebastian , de Kort Paul L.M. , Koudstaal Peter J. , Lim Jae-Sung , Makin Stephen D.J. , Mok Vincent C.T. , van Oostenbrugge Robert J. , Roussel Martine , Staals Julie , Valdés-Hernández Maria del C. , Venketasubramanian Narayanaswamy , Verhey Frans R.J. , Wardlaw Joanna M. , Werring David J. , Xu Xin , van Zandvoort Martine J.E. , Biesbroek J. Matthijs , Chappell Francesca M. , Biessels Geert Jan
Published: August 2023

BACKGROUND:

Poststroke cognitive impairment (PSCI) occurs in about half of stroke survivors. Cumulative evidence indicates that functional outcomes of stroke are worse in women than men. Yet it is unknown whether the occurrence and characteristics of PSCI differ between men and women.

METHODS:

Individual patient data from 9 cohorts of patients with ischemic stroke were harmonized and pooled through the Meta-VCI-Map consortium (n=2343, 38% women). We included patients with visible symptomatic infarcts on computed tomography/magnetic resonance imaging and cognitive assessment within 15 months after stroke. PSCI was defined as impairment in ≥1 cognitive domains on neuropsychological assessment. Logistic regression analyses were performed to compare men to women, adjusted for study cohort, to obtain odds ratios for PSCI and individual cognitive domains. We also explored sensitivity and specificity of cognitive screening tools for detecting PSCI, according to sex (Mini-Mental State Examination, 4 cohorts, n=1814; Montreal Cognitive Assessment, 3 cohorts, n=278).

RESULTS:

PSCI was found in 51% of both women and men. Men had a lower risk of impairment of attention and executive functioning (men: odds ratio, 0.76 [95% CI, 0.61–0.96]), and language (men: odds ratio, 0.67 [95% CI, 0.45–0.85]), but a higher risk of verbal memory impairment (men: odds ratio, 1.43 [95% CI, 1.17–1.75]). The sensitivity of Mini-Mental State Examination (<25) for PSCI was higher for women (0.53) than for men (0.27;
P
=0.02), with a lower specificity for women (0.80) than men (0.96;
P
=0.01). Sensitivity and specificity of Montreal Cognitive Assessment (<26.) for PSCI was comparable between women and men (0.91 versus 0.86;
P
=0.62 and 0.29 versus 0.28;
P
=0.86, respectively).

CONCLUSIONS:

Sex was not associated with PSCI occurrence but affected domains differed between men and women. The latter may explain why sensitivity of the Mini-Mental State Examination for detecting PSCI was higher in women with a lower specificity compared with men. These sex differences need to be considered when screening for and diagnosing PSCI in clinical practice.
Dogs Rely On Visual Cues Rather Than On Effector-Specific Movement Representations to Predict Human Action Targets
Authors: Lonardo Lucrezia , Völter Christoph J. , Lamm Claus , Huber Ludwig
Published: August 2023

The ability to predict others’ actions is one of the main pillars of social cognition. We investigated the processes underlying this ability by pitting motor representations of the observed movements against visual familiarity. In two pre-registered eye-tracking experiments, we measured the gaze arrival times of 16 dogs (Canis familiaris) who observed videos of a human or a conspecific executing the same goal-directed actions. On the first trial, when the human agent performed human-typical movements outside dogs’ specific motor repertoire, dogs’ gaze arrived at the target object anticipatorily (i.e., before the human touched the target object). When the agent was a conspecific, dogs’ gaze arrived to the target object reactively (i.e., upon or after touch). When the human agent performed unusual movements more closely related to the dogs’ motor possibilities (e.g., crawling instead of walking), dogs’ gaze arrival times were intermediate between the other two conditions. In a replication experiment, with slightly different stimuli, dogs’ looks to the target object were neither significantly predictive nor reactive, irrespective of the agent. However, when including looks at the target object that were not preceded by looks to the agents, on average dogs looked anticipatorily and sooner at the human agent’s action target than at the conspecific’s. Looking times and pupil size analyses suggest that the dogs’ attention was captured more by the dog agent. These results suggest that visual familiarity with the observed action and saliency of the agent had a stronger influence on the dogs’ looking behaviour than effector-specific movement representations in anticipating action targets.
Five new species of Pleurothallis (Orchidaceae: Pleurothallidinae) in subsection Macrophyllae-Fasciculatae from Southeastern Ecuador
Authors: JIMÉNEZ MARCO M. , VÉLEZ-ABARCA LEISBERTH , MASHENDO-JIMBICTI VIVIANA , GARZÓN-SUÁREZ HENRY X. , MONTEROS MARCO F. , WILSON MARK
Published: August 2023

Five new species of Pleurothallis subsect. Macrophyllae-Fasciculatae from Ecuador are described and illustrated: P. kashi-menkakarai, P. lapoi, P. marioandresavilae, P. sabanillae and P. tinajillensis. The taxonomic similarities of the new entities are discussed and information about their distribution, habitat, and conservation status is provided. Additionally, the first known and confirmed locality for Pleurothallis saueri is provided, a previously described species without collection data, as well as a lectotype is designated for this name.
Characteristics of Lake-Effect Precipitation over the Black River Valley and Western Adirondack Mountains
Authors: Steenburgh W. James , Cunningham Julie A. , Bergmaier Philip T. , Geerts Bart , Veals Peter
Published: August 2023

Potential factors affecting the inland penetration and orographic modulation of lake-effect precipitation east of Lake Ontario include the environmental (lake, land, and atmospheric) conditions, mode of the lake-effect system, and orographic processes associated with flow across the downstream Tug Hill Plateau (herein Tug Hill), Black River valley, and Adirondack Mountains (herein Adirondacks). In this study we use data from the KTYX WSR-88D, ERA5 reanalysis, New York State Mesonet, and Ontario Winter Lake-effect Systems (OWLeS) field campaign to examine how these factors influence lake-effect characteristics with emphasis on the region downstream of Tug Hill. During an eight-cool-season (16 November–15 April) study period (2012/13–2019/20), total radar-estimated precipitation during lake-effect periods increased gradually from Lake Ontario to upper Tug Hill and decreased abruptly where the Tug Hill escarpment drops into the Black River valley. The axis of maximum precipitation shifted poleward across the northern Black River valley and into the northwestern Adirondacks. In the western Adirondacks, the heaviest lake-effect snowfall periods featured strong, near-zonal boundary layer flow, a deep boundary layer, and a single precipitation band aligned along the long-lake axis. Airborne profiling radar observations collected during OWLeS IOP10 revealed precipitation enhancement over Tug Hill, spillover and shadowing in the Black River valley where a resonant lee wave was present, and precipitation invigoration over the western Adirondacks. These results illustrate the orographic modulation of inland-penetrating lake-effect systems downstream of Lake Ontario and the factors favoring heavy snowfall over the western Adirondacks.

Significance Statement

Inland penetrating lake-effect storms east of Lake Ontario affect remote rural communities, enable a regional winter-sports economy, and contribute to a snowpack that contributes to runoff and flooding during thaws and rain-on-snow events. In this study we illustrate how the region’s three major geographic features—Tug Hill, the Black River valley, and the western Adirondacks—affect the characteristics of lake-effect precipitation, describe the factors contributing to heavy snowfall over the western Adirondacks, and provide an examples of terrain effects in a lake-effect storm observed with a specially instrumented research aircraft.
Environmental Controls on the Tropical Island Diurnal Cycle in the Context of Intraseasonal Variability
Authors: Natoli Michael B. , Maloney Eric D.
Published: August 2023

The mechanisms regulating the relationship between the tropical island diurnal cycle and large-scale modes of tropical variability such as the boreal summer intraseasonal oscillation (BSISO) are explored in observations and an idealized model. Specifically, the local environmental conditions associated with diurnal cycle variability are explored. Using Luzon Island in the northern Philippines as an observational test case, a novel probabilistic framework is applied to improve the understanding of diurnal cycle variability. High-amplitude diurnal cycle days tend to occur with weak to moderate offshore low-level wind and near to above average column moisture in the local environment. The transition from the BSISO suppressed phase to the active phase is most likely to produce the wind and moisture conditions supportive of a substantial diurnal cycle over western Luzon and the South China Sea (SCS). Thus, the impact of the BSISO on the local diurnal cycle can be understood in terms of the change in the probability of favorable environmental conditions. Idealized high-resolution 3D Cloud Model 1 (CM1) simulations driven by base states derived from BSISO composite profiles are able to reproduce several important features of the observed diurnal cycle variability with BSISO phase, including the strong, land-based diurnal cycle and offshore propagation in the transition phases. Background wind appears to be the primary variable controlling the diurnal cycle response, but ambient moisture distinctly reduces precipitation strength in the suppressed BSISO phase and enhances it in the active phase.
Observations of Surface Gravity Wave Spectra from Moving Platforms
Authors: Colosi Luke , Pizzo Nick , Grare Laurent , Statom Nick , Lenain Luc
Published: August 2023

Surface waves play an important role in the ocean–atmosphere coupled climate system by mediating the exchange of momentum, heat, and gas between the atmosphere and the ocean. Pseudo-Lagrangian autonomous platforms (e.g., Boeing Liquid Robotics Wave Gliders) have been used to investigate the underlying physical dynamics involved in these processes to better parameterize the air–sea exchange occurring at the scale of the surface waves. This requires accurate measurements of directional surface waves down to short scales [O(1) m], as these shorter waves support most of the stress between the atmosphere and the ocean. A challenge to overcome for pseudo-Lagrangian autonomous vehicles is that the platform’s velocity causes the observed frequency of the waves to be Doppler shifted. This leads to a modulation of the wave spectrum, particularly at high frequencies, that depends on the platform’s speed, the wave frequency, and the relative angle between the direction of wave and platform propagation. In this work, we propose a method to account for Doppler effects that considers the full directionality of the wave field. The method is validated using a unique dataset collected from a fleet of two Wave Gliders off the coast of Southern California in September 2019 operating on the perimeter of a tight square (500-m edge length) track over a 3-day deployment. This technique can be used to estimate wave spectra derived from other slow-moving surface vehicles such as Saildrones that use platform motion to characterize the surface wave field. MATLAB routines to implement this method are publicly available.

Significance Statement

The purpose of this study is to introduce a general approach that corrects observations of ocean surface waves collected on board autonomous surface vehicles (ASVs) for the effects on the wave period due to the vehicle’s forward motion. This is important because improving climate models requires accurate measurements of short-wavelength waves, which can be readily obtained from ASVs. Our method provides the tools for ASVs to better understand air–sea physics and the larger role ocean surface waves play in Earth’s climate system.
Technology of Interaction Between School and Family in the Education of Primary School Age Children
Authors: Ansabayeva Ainur , Mailybaeva Gulmira , Utegulov Daniyar , Temerbayeva Zhanna , Nugmanova Farida
Published: August 2023

Research on child-rearing technologies is always relevant in pedagogical science. The purpose of this study is to establish the relationship between the school and the family in the educational process of primary school age children. To investigate this issue, the study employed the following methods: comparative analysis, synthesis, logical analysis, modeling, deduction, analysis of scientific literature, and the study of student performance. The results of this study are the theoretical and practical foundations of the relationship between school and family in the course of raising a child, establishing common, and distinctive features in the approaches. The practical value of this study lies in the fact that the proposed means and methods of educating children of primary school age can be used by parents and teachers to improve the establishment of contact with the child and effectively influence on them, to form the student as a full-fledged participant in social relations.
The 2023 A. Hamblin Letton Lecture, Southeastern Surgical Conference Lecture “Choices Matter in Cancer Surgery: A Life’s Journey”
Authors: Tseng Jennifer F.
Published: August 2023

Attaining a successful career in cancer surgery depends on many choices. In this paper based on the Letton Lecture from the 2023 Southeastern Surgical Conference, Dr. Jennifer Tseng reflects on the choices that enabled her current work.