bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomic Revealed That Selenium-Rich Lactobacillus plantarum Alleviated Cadmium-Induced Immune Responses in Bulatmai barbel Luciobarbus capito Kidneys
Authors: Sun Qingsong , Pang Yuran , Qin Yuhan , Dong Ziting , Ma Yanling , Zhao Yuan , Zhang Zhanning , Liu Jinmei , Mao Binghui , Yin Baishuang
Published: June 2024

Cadmium (Cd) is a common environmental pollutant that accumulates mainly in the kidneys and thus endangers the physiological health of aquatic animals. Selenium (Se) is a natural antidote to heavy metals that antagonises heavy metal toxicity and enhances the antioxidant capacity of organisms. Lactobacillus plantarum (L. plantarum) can reduce the toxicity of heavy metals through adsorption, reduction and metabolism. Studies have confirmed that the biological synthesis of Se nanoparticles (Bio-SeNPs) using bacterial microorganisms is simple, safe and less toxic than the synthesis of inorganic and organic Se, but the effect on Cd-induced immunosuppression is un-known. One hundred and eighty Bulatmai barbel (Luciobarbus capito: L. capito) plants were randomly divided into control (C), Cd and Cd + Se-enriched L. plantarum groups (S1L1-Cd) and fed for 28 days. The analysis methods included histopathology, test kits, transcriptomics and real-time quantitative PCR. The addition of selenium-enriched L. plantarum significantly attenuated cadmium-induced pathological changes such as glomerular atrophy, detachment of renal tubular epithelial cells, mild swelling, and interstitial inflammatory cell infiltration. Cd stress can lead to significant decreases in RBC, HCT, WBC, LZM, C3, and IgM levels, and the addition of Se-enriched L. plantarum can significantly reverse the changes in these indicators. Transcriptomic analysis revealed 488 DEGs in the Cd groups, 301 of which were upregulated and 187 of which were downregulated. There were 1474 DEGs in the S1L1-Cd group, of which 720 were upregulated and 754 were downregulated. In addition, GO enrichment analysis revealed that the biological regulation of the most differentially expressed genes involved metal ion binding, ATP binding and nucleotide inclusion. KEGG enrichment analysis revealed six of the most enriched pathways: oxidative phosphorylation, Huntington disease, retrograde endocannabinoid signalling, natural killer cell-mediated cyto-toxicity, the IL-17 signalling pathway, and leukocyte transient migration. Moreover, we selected 12 DEGs for qRT-PCR, which showed that the qRT-PCR results were consistent with our RNA-Seq results. Our results suggest that Se-enriched L. plantarum can enhance immunity and alleviate Cd exposure-mediated immunosuppression in L. capito.
Regional Variability in Sugar and Amino Acid Content of U.S. Soybeans and the Impact of Autoclaving on Reducing Sugars and Free Lysine
Authors: Murai Takehiro , Naeve Seth , Annor George A.
Published: June 2024

Exploring the sugar and amino acid content variability and the influence of thermal processing on these in soybeans can help optimize their utilization in animal feed. This study examined 209 samples harvested in 2020 and 55 samples harvested in 2021 from across the U.S. to assess their sugar variability and amino acid variability. Harvest regions included the East Corn Belt, West Corn Belt, Mid-South, East Coast, and the Southeast of the U.S. In addition to the sugar and amino acid contents, protein, oil, and seed size were also analyzed. Samples from 2021 were evaluated for their sugar and amino acid contents before and after autoclaving the seeds at 105–110 °C for 15 min. For the samples harvested in 2020, sucrose (4.45 g 100 g−1) and stachyose (1.34 g 100 g−1) were the most prevalent sugars. For the samples harvested in 2021, L-arginine (9.82 g 100 g−1), leucine (5.29 g 100 g−1), and glutamate (4.90 g 100 g−1) were the most prevalent amino acids. Heat treatment resulted in an 8.47%, 20.88%, 11.18%, and 1.46% median loss of free lysine, sucrose, glucose, and fructose. This study’s insights into the variability in sugar and amino acid content and the heat-induced changes in the nutritional composition of soybeans provide a reference for improving soybean quality assessment and optimizing its use in animal feed formulations in the U.S.
Evaluation of Methylene Blue Migration from Time—Temperature Indicators Using LC-MS/MS
Authors: Lee Seung-Eun , Lim Kyung-Jik , Lee Yoon-Hee , Shin Han-Seung
Published: June 2024

The purpose of this study was to evaluate and validate methylene blue migration from printed time–temperature indicators (TTIs) into food. It also highlights the importance of establishing regulatory measures and safety standards for food packaging, suggesting that this can contribute to improving food packaging safety. Liquid chromatography–mass spectrometry (LC-MS/MS) was used to quantify methylene blue migration in various food simulant and food matrix samples. The results show that the level of methylene blue migration varies significantly depending on the chemical properties of the food mimetic and the composition of the food matrix. The established method demonstrated a high sensitivity, with limits of detection (LODs) of 0.0019–0.0706 μg/L (kg) and limits of quantification (LOQs) of 0.0057–0.2138 μg/L (kg). This study highlights the need for a regulatory framework to mitigate the health risks associated with methylene blue in intelligent packaging systems and argues that regulatory thresholds should be set to ensure food safety and quality.
Post-Harvest Behavior of Seedless Conical and Mini-Conical Peppers: Weight Loss, Dry Matter Content, and Total Soluble Solids as Indicators of Quality and Commercial Shelf-Life
Authors: Díaz-Pérez Manuel , Hernández-García José Javier , Carreño-Ortega Ángel , Velázquez Martí Borja
Published: June 2024

This study aimed to assess the post-harvest dynamics of seedless conical and mini-conical pepper cultivars in terms of fruit weight loss, dry matter content, and soluble solid content. The above parameters were demonstrated to be effective commercial pepper shelf-life indicators. The commercial quality of pepper fruit intended for export was evaluated weekly under simulated fruit storage conditions for over 28 d. Results revealed that fruit weight loss, dry matter content, and soluble solid content were affected by cultivar type and storage duration. Additionally, a strong correlation between these variables was observed confirming their linear relationship which was more profound between dry matter and total soluble solid content. Daily changes during storage were similar in both seedless conical and mini-conical peppers, while the fruit weight loss daily rate was greater than that of dry matter. Water loss was identified to be the main factor causing reduced fruit quality. Solid content reduction occurred predominately during the initial storage period. Notably, fruit with lower dry matter content at harvest tended to maintain their commercial quality for a longer time due to their ability to resist water loss without any visible signs of deterioration, which is beneficial during prolonged storage.
A Comprehensive Study for Determination of Free Fatty Acids in Selected Biological Materials: A Review
Authors: Uçar Beyza , Gholami Zahra , Svobodová Kateřina , Hradecká Ivana , Hönig Vladimír
Published: June 2024

The quality of oil is highly dependent on its free fatty acid (FFA) content, especially due to increased restrictions on renewable fuels. As a result, there has been a growing interest in free fatty acid determination methods over the last few decades. While various standard methods are currently available, such as the American Oil Chemists Society (AOCS), International Union of Pure and Applied Chemistry (IUPAC), and Japan Oil Chemists’ Society (JOCS), to obtain accurate results, there is a pressing need to investigate a fast, accurate, feasible, and eco-friendly methodology for determining FFA in biological materials. This is owing to inadequate characteristics of the methods, such as solvent consumption and reproducibility, among others. This study aims to investigate FFA determination methods to identify suitable approaches and introduce a fresh perspective.
Impact of Fibrin Gel Architecture on Hepatocyte Growth Factor Release and Its Role in Modulating Cell Behavior for Tissue Regeneration
Authors: Wein Svenja , Jung Shannon Anna , Al Enezy-Ulbrich Miriam Aischa , Reicher Luca , Rütten Stephan , Kühnel Mark , Jonigk Danny , Jahnen-Dechent Wilhelm , Pich Andrij , Neuss Sabine
Published: June 2024

A novel scaffold design has been created to enhance tissue engineering and regenerative medicine by optimizing the controlled, prolonged release of Hepatocyte Growth Factor (HGF), a powerful chemoattractant for endogenous mesenchymal stem cells. We present a new stacked scaffold that is made up of three different fibrin gel layers, each of which has HGF integrated into the matrix. The design attempts to preserve HGF’s regenerative properties for long periods of time, which is necessary for complex tissue regeneration. These multi-layered fibrin gels have been mechanically evaluated using rheometry, and their degradation behavior has been studied using D-Dimer ELISA. Understanding the kinetics of HGF release from this novel scaffold configuration is essential for understanding HGF’s long-term sustained bioactivity. A range of cell-based tests were carried out to verify the functionality of HGF following extended incorporation. These tests included 2-photon microscopy using phalloidin staining to examine cellular morphology, SEM analysis for scaffold–cell interactions, and scratch and scatter assays to assess migration and motility. The analyses show that the novel stacking scaffold promotes vital cellular processes for tissue regeneration in addition to supporting HGF’s bioactivity. This scaffold design was developed for in situ tissue engineering. Using the body as a bioreactor, the scaffold should recruit mesenchymal stem cells from their niche, thus combining the regenerative abilities of HGF and MSCs to promote tissue remodeling and wound repair.
Advances in Hydrogels of Drug Delivery Systems for the Local Treatment of Brain Tumors
Authors: Yang Jingru , Wang Zhijie , Ma Chenyan , Tang Hongyu , Hao Haoyang , Li Mengyao , Luo Xianwei , Yang Mingxin , Gao Liang , Li Juan
Published: June 2024

The management of brain tumors presents numerous challenges, despite the employment of multimodal therapies including surgical intervention, radiotherapy, chemotherapy, and immunotherapy. Owing to the distinct location of brain tumors and the presence of the blood–brain barrier (BBB), these tumors exhibit considerable heterogeneity and invasiveness at the histological level. Recent advancements in hydrogel research for the local treatment of brain tumors have sought to overcome the primary challenge of delivering therapeutics past the BBB, thereby ensuring efficient accumulation within brain tumor tissues. This article elaborates on various hydrogel-based delivery vectors, examining their efficacy in the local treatment of brain tumors. Additionally, it reviews the fundamental principles involved in designing intelligent hydrogels that can circumvent the BBB and penetrate larger tumor areas, thereby facilitating precise, controlled drug release. Hydrogel-based drug delivery systems (DDSs) are posited to offer a groundbreaking approach to addressing the challenges and limitations inherent in traditional oncological therapies, which are significantly impeded by the unique structural and pathological characteristics of brain tumors.
Early Uses by Ancient Hawaiians, and Environmental, Geographical, and Ecological History, of Haleakalā Crater, East Maui
Authors: Pérez Francisco Luis
Published: June 2024

This research assesses the environmental uses of Haleakalā Crater, and adjoining east Maui areas, by native Hawaiians during recent centuries, and evaluates the modern utilization of this volcanic depression. The study methods examine, and focus on, numerous historical and modern accounts and reports. Three historical periods were identified as follows: (1). A phase, from the ~1400s until contact with foreigners in 1778, characterized by the development of settlements, population expansion, the intensification of traditional agriculture, and the political consolidation of Maui. The construction of the Kiha-a-Pi‘i-lani trail across the crater enhanced travel between distant island areas. (2). Following the collapse of Maui’s human populations during the late 1700s, and until the 20th century, came a period distinguished by scientific exploration of the crater, and intentional or accidental introductions of animals, including goats, horses, cattle, and wild dogs. (3). After Haleakalā became a National Park in 1916, efforts to eradicate introduced animals and to encourage the reproduction of silverswords and other plants were initiated. Unfortunately, in retrospect, construction of a modern paved road, improvement of hiking trails, and building of facilities allowed swift access to the Park and substantially increased tourist numbers, up to ~2 million yearly visitors. Faced with such crowds, the long-term planning of Park resources appears to be a formidable task.
The Role of Family or Community Nurse in Dealing with Frail and Chronic Patients in Italy: A Scoping Review
Authors: Scrimaglia Susan , Ricci Matteo , Masini Alice , Montalti Marco , Conti Andrea , Camedda Claudia , Panella Massimiliano , Dallolio Laura , Longobucco Yari
Published: June 2024

Even though Family and Community Nurses (FCNs) were introduced into the Italian healthcare system in 2000, to date, there is a substantial knowledge gap regarding the implementation of these professional figures within the Primary Care (PC) system. This scoping review aims to provide a comprehensive picture of the role of FCNs in managing older adults and the elderly with chronic conditions within the Italian PC system. A search on Medline, Cumulative Index to Nursing and Allied Health Literature, Cochrane Library, Embase, and Scopus was conducted including studies published until 7 April 2023. Among 141 potential articles, only 4 met our inclusion criteria. Each of these studies attributed their findings to the presence of FCNs. They reported a significant decrease in the prevalence of several behaviours among patients diagnosed with hypertension, a reduction in metabolic complications among frail patients receiving home enteral nutrition, a decline in hospital readmissions or emergency services utilization among patients >65 years of ages with at least one chronic disease, and, notably, a high level of effectiveness in detecting major cardiovascular events in patients with cardiac implantable electronic devices. Despite the effectiveness of interventions managed by FCNs, comprehensive information and research on the integration of this role within the PC setting are still lacking in the Italian healthcare system.
Systemic and Oral Characteristics of Convalescent Inpatients Requiring Oral-Health Management by a Dental Specialist during Hospitalization
Authors: Todayama Naoki , Hara Ryuzo , Tabata Tomohiro , Hatanaka Yukiko , Mukai Tomoko , Someya Mika , Kuwazawa Miki , Suzuki Hiroyuki , Hironaka Shouji , Kawate Nobuyuki , Furuya Junichi
Published: June 2024

Older adults often experience poor oral functions, hindering rehabilitation post-acute disease treatment. However, characteristics of hospitalized patients who would benefit from professional oral-health management (POHM) have not been clarified. Therefore, we aimed to elucidate systemic and oral characteristics of patients requiring POHM during hospitalization in a convalescent hospital. This study included 312 participants admitted to the rehabilitation department of a convalescent hospital for a year. The patients were categorized according to POHM requirements (no-POHM group: 137 patients; POHM group: 175 patients) by discharge. Age, sex, primary disease at admission, Glasgow coma scale (GCS), Functional Independence Measurement (FIM), Mini nutritional assessment-short form (MNA-SF), Functional oral intake scale (FOIS), number of present and functional teeth, Oral Health Assessment Tool (OHAT) scores, and POHM details provided during patient hospitalization were compared. Binomial logistic-regression analysis identified patients requiring POHM as those who had suffered a stroke and had a low number of present teeth, poor overall oral health, low food form, and low motor skills at admission. A high percentage of POHM interventions comprised oral-hygiene care and denture treatment. In summary, patients whose oral health has deteriorated and those experiencing oral-intake difficulties upon admission to a convalescent hospital may require oral-health management.
A New Plant-Wearable Sap Flow Sensor Reveals the Dynamic Water Distribution during Watermelon Fruit Development
Authors: Zhang Runqing , Chai Yangfan , Liang Xinyu , Liu Xiangjiang , Wang Xiaozhi , Hu Zhongyuan
Published: June 2024

This study utilized a plant-wearable sap flow sensor developed by a multidisciplinary team at Zhejiang University to monitor water distribution patterns in watermelon fruit stalks throughout their developmental stages. The dynamic rules of sap flow at different stages of fruit development were discovered: (1) In the first stage, sap flow into the fruit gradually halts after sunrise due to increased leaf transpiration, followed by a rapid increase post-noon until the next morning, correlating with fruit expansion. (2) In the second stage, the time of inflow sap from noon to night is significantly shortened, while the outflow sap from fruit is observed with the enhancement of leaf transpiration after sunrise, which is consistent with the slow fruit growth at this stage. (3) In the third stage, the sap flow maintains the diurnal pattern. However, the sap flow that inputs the fruit at night is basically equal to the sap flow that outputs the fruit during the day; the fruit phenotype does not change anymore. In addition, a strong correlation between the daily mass growth in fruit and the daily sap flow amount in fruit stalk was identified, validating the sensor’s utility for fruit growth monitoring and yield prediction.
Assessing High-Density Lipoprotein: Shifting Focus from Quantity to Quality in Cardiovascular Disease Risk Assessment
Authors: Ahmed Tanvir , Bowden Rodney G.
Published: June 2024

High-density lipoprotein cholesterol (HDL) has long been regarded as a protective factor against cardiovascular disease (CVD). However, recent research challenges this notion, suggesting that HDL functionality rather than its quantity may be a more accurate predictor of CVD risk. While epidemiological studies have traditionally found that higher HDL levels are associated with reduced CVD risk, intervention trials aiming to elevate HDL levels have yielded inconsistent results. Moreover, observational studies have reported that unusually high HDL levels are associated with increased mortality rates. These discrepancies underscore the complexity of the role of HDL in CVD. Reverse cholesterol transport, facilitated by HDL, plays a crucial role in preventing atherosclerosis by removing cholesterol from peripheral tissues. Additionally, HDL exhibits anti-inflammatory properties by inhibiting endothelial adhesion molecules and suppressing pro-inflammatory cytokines. Recent studies have highlighted the importance of HDL particle number, size, and functionality in assessing CVD risk. For instance, increased HDL particle number and larger particle size have been associated with reduced CVD risk, independent of HDL cholesterol levels. Furthermore, HDL’s cholesterol efflux capacity has emerged as a promising biomarker for predicting CVD risk, with higher efflux capacity correlating with lower CVD incidence and mortality. This article reviews the latest findings regarding the role of HDL in CVD risk assessment, emphasizing the need to focus on HDL quantity and HDL quality.
Low-Temperature Fast Firing Preparation of Zn2TiO4 Crystalline Photovoltaic Glass Ink and Its Properties
Authors: Chen Yongjian , Dong Weixia , Bao Qifu , Zhao Tiangui , Cheng Zhipeng , Xu Yan
Published: June 2024

A Zn2TiO4 crystalline photovoltaic glass ink was prepared by fast firing at 700 °C for 5 min by the glass crystallisation method, which effectively improved the reflectivity and acid resistance of the photovoltaic glass ink coating. The phase, morphology and properties of the samples were tested by XRD, SEM and UV-vis diffuse reflection, etc. The enhanced reflectivity mechanism was proposed. The results showed that the increase in ZnO/SiO2 ratio reduced the transition temperature (Tg) and crystallisation temperature (Tp) of the glass melt, which could promote the crystallinity of Zn2TiO4 in photovoltaic glass ink coatings and thus improve the acid resistance of photovoltaic glass inks. Significant improvement in reflectance and whiteness is due to the Zn2TiO4 crystallinity growth, which fills in the pores of the ink surface, and TiO2 fillers keep almost the same surface roughness (0.2 µm) and wetting angle (5.2°). Typical samples achieved 89.2% of the whiteness and 88.0% of the reflectance, and the weight loss in acid was 3.9 mg/cm2, which could improve the efficiency of solar power generation.
Empagliflozin and Dapagliflozin Improve Endothelial Function in Mexican Patients with Type 2 Diabetes Mellitus: A Double-Blind Clinical Trial
Authors: Balleza Alejandri Luis Ricardo , Grover Páez Fernando , González Campos Erick , Ramos Becerra Carlos G. , Cardona Muñóz Ernesto Germán , Pascoe González Sara , Ramos Zavala María Guadalupe , Reynoso Roa Africa Samantha , Suárez Rico Daniel Osmar , Beltrán Ramírez Alberto , García Galindo Jesús Jonathan , Cardona Müller David , Galán Ruíz Claudia Yanette
Published: June 2024

Aim: To assess the acute effect of empagliflozin versus dapagliflozin administration on flow-mediated vasodilation in patients with type 2 diabetes mellitus. Design: A double-blind clinical trial, at the Experimental and Clinical Therapeutics Institute, University Health Sciences Center, at the Universidad de Guadalajara, in inpatients with T2D according to the 2023 ADA criteria. Methods: Thirty patients (15 males and 15 females), aged between 35 and 65 years, were included in this study, according to the 2023 ADA criteria. The eligible patients were randomly assigned to three groups: empagliflozin 25 mg once daily, dapagliflozin 10 mg once daily, or placebo once daily. Anthropometric parameters were taken using validated techniques. FMD was measured using a high-resolution semiautomatic ultrasound UNEX-EF 38G (UNEX Co., Ltd., Nagoya, Japan). Arterial tension was determined with the OMRON electronic digital sphygmomanometer (HEM 907 XL, Kyoto, Japan). Results: The group of patients who received empagliflozin had a significantly lower baseline flow-mediated dilation (FMD) compared to the group receiving dapagliflozin (p = 0.017); at the end of this study, the empagliflozin group achieved a comparable FMD to the dapagliflozin group (p = 0.88). Conclusion: After the treatment period, the empagliflozin and dapagliflozin groups achieved similar FMD, suggesting a class effect.
The Effect of Physical Therapy on Somatosensory Tinnitus
Authors: Yu Hong-Zhe , Gong Jia-Min , Hong Guo-Wei , Zhou Ruo-Qiao , Fu Xin-Ping , Fan Ting , Zheng Yu-Qing , Peng Ying-Qiu , Li Jian , Wang Yun-Feng
Published: June 2024

Objective: The objective of this work was to assess the effect of physical therapy in patients with somatosensory tinnitus (ST) and explore the influence of physical therapy on clinical variables obtained before treatment. Methods: A total of 43 patients with ST were randomized to the immediate-start group (n = 20) and delayed-start group (n = 23). All patients received physical therapy for 1 week (seven sessions). Each session lasted 60 min. The Visual Analogue Scale (VAS), Tinnitus Handicap Inventory (THI), and numerical pain rating scale (NPRS) scores were documented at baseline and after treatment (week 1) for all patients. For subjects in the immediate-start group, the THI, VAS, and NPRS scores were measured after therapy (weeks 6, 9, and 12, respectively). Medical history characteristic functional activity scale (HCFA) scores were measured at baseline to assess the association between somatic symptoms and tinnitus. Results: At week 1, VAS, THI, and NPRS scores of patients in the immediate-start group were improved by 1.25 ± 1.59, 11.10 ± 15.10, and 0.95 ± 1.54 points, respectively, and were significantly higher than those in the delayed-start group (p < 0.05). The change in VAS, THI, and NPRS scores in the treatment group was significantly positively correlated with the scores of the HCFA before treatment (r = 0.786, p < 0.001; r = 0.680, p = 0.001; r = 0.796, p < 0.001). There was no significant difference in THI, VAS, and NPRS scores among patients in the immediate-start group between weeks 1, 6, 9, and 12 after treatment (p > 0.05). Conclusions: Although more participants were necessary in the further study, the study implies that physical therapy can reduce physical pain, improve tinnitus symptoms, and quality of life in ST patients without hearing loss, and the short-term curative effect is stable, especially for tinnitus patients with clear somatic symptoms.
Outcomes and Impact of Pre-ECMO Clinical Course in Severe COVID-19-Related ARDS Treated with VV-ECMO: Data from an Italian Referral ECMO Center
Authors: Sales Gabriele , Montrucchio Giorgia , Sanna Valentina , Collino Francesca , Fanelli Vito , Filippini Claudia , Simonetti Umberto , Bonetto Chiara , Morscio Monica , Verderosa Ivo , Urbino Rosario , Brazzi Luca
Published: June 2024

Background: The efficacy of veno-venous extracorporeal membrane oxygenation (VV-ECMO) as rescue therapy for refractory COVID-19-related ARDS (C-ARDS) is still debated. We describe the cohort of C-ARDS patients treated with VV-ECMO at our ECMO center, focusing on factors that may affect in-hospital mortality and describing the time course of lung mechanics to assess prognosis. Methods: We performed a prospective observational study in the intensive care unit at the “Città della Salute e della Scienza” University Hospital in Turin, Italy, between March 2020 and December 2021. Indications and management of ECMO followed the Extracorporeal Life Support Organization (ELSO) guidelines. Results: The 60-day in-hospital mortality was particularly high (85.4%). Non-survivor patients were more frequently treated with non-invasive ventilatory support and steroids before ECMO (95.1% vs. 57.1%, p = 0.018 and 73.2% vs. 28.6%, p = 0.033, respectively), while hypertension was the only pre-ECMO factor independently associated with in-hospital mortality (HR: 2.06, 95%CI: 1.06–4.00). High rates of bleeding (85.4%) and superinfections (91.7%) were recorded during ECMO, likely affecting the overall length of ECMO (18 days, IQR: 10–24) and the hospital stay (32 days, IQR: 24–47). Static lung compliance was lower in non-survivors (p = 0.031) and differed over time (p = 0.049), decreasing by 48% compared to initial values in non-survivors. Conclusions: Our data suggest the importance of considering NIS among the common ECMO eligibility criteria and changes in lung compliance during ECMO as a prognostic marker.
Development and Evaluation of 3D-Printed PLA/PHA/PHB/HA Composite Scaffolds for Enhanced Tissue-Engineering Applications
Authors: Raziyan Motahareh Sadat , Palevicius Arvydas , Perkowski Dariusz , Urbaite Sigita , Janusas Giedrius
Published: June 2024

Recently, tissue engineering has been revolutionised by the development of 3D-printed scaffolds, which allow one to construct a precise architecture with tailored properties. In this study, three different composite materials were synthesised using a combination of polylactic acid (PLA), polyhydroxyalkanoates (PHA), poly(3-hydroxybutyrate) (PHB) and hydroxyapatite (HA) in varying weight percentages. Morphological properties were evaluated by scanning electron microscopy showing a uniform distribution of HA particles throughout the matrix, indicating good compatibility between the materials. Furthermore, the printed scaffolds were tested under pressure using a load cell to examine mechanical strength. Scanning electron microscopy (SEM) analysis showed favorable dispersion, biological compatibility together with enhanced bioactivity within the PHB/PHA/PLA/HA composite matrixes. Thus, this paper demonstrates the successful design and implementation of these composite structures for tissue-engineering applications and highlights the effective development of biocompatible scaffold designs with improved functionality.
The Use of the GWPCA-MGWR Model for Studying Spatial Relationships between Environmental Variables and Longline Catches of Yellowfin Tunas
Authors: Li Menghao , Yang Xiaoming , Wang Yue , Wang Yuhan , Zhu Jiangfeng
Published: June 2024

The yellowfin tuna represents a significant fishery resource in the Pacific Ocean. Its resource endowment status and spatial variation mechanisms are intricately influenced by marine environments, particularly under varying climate events. Consequently, investigating the spatial variation patterns of dominant environmental factors under diverse climate conditions, and understanding the response of yellowfin tuna catch volume based on the spatial heterogeneity among these environmental factors, presents a formidable challenge. This paper utilizes comprehensive 5°×5° yellowfin tuna longline fishing data and environmental data, including seawater temperature and salinity, published by the Western and Central Pacific Fisheries Commission (WCPFC) and the Inter-American Tropical Tuna Commission (IATTC) for the period 2000–2021 in the Pacific Ocean. In conjunction with the Niño index, a multiscale geographically weighted regression model based on geographically weighted principal component analysis (GWPCA-MGWR) and spatial association between zones (SABZ) is employed for this study. The results indicate the following: (1) The spatial distribution of dominant environmental factors affecting the catch of Pacific yellowfin tuna is primarily divided into two types: seawater temperature dominates in the western Pacific Ocean, while salinity dominates in the eastern Pacific Ocean. When El Niño occurs, the area with seawater temperature as the dominant environmental factor in the western Pacific Ocean further extends eastward, and the water layers where the dominant environmental factors are located develop to deeper depths; when La Niña occurs, there is a clear westward expansion in the area with seawater salinity as the dominant factor in the eastern Pacific Ocean. This change in the spatial distribution pattern of dominant factors is closely related to the movement of the position of the warm pool and cold tongue under ENSO events. (2) The areas with a higher catch of Pacific yellowfin tuna are spatially associated with the dominant environmental factor of mid-deep seawater temperature (105–155 m temperature) to a greater extent than other factors, the highest correlation exceeds 70%, and remain relatively stable under different ENSO events. The formation of this spatial association pattern is related to the vertical movement of yellowfin tuna as affected by subsurface seawater temperature. (3) The GWPCA-MGWR model can fully capture the differences in environmental variability among subregions in the Pacific Ocean under different climatic backgrounds, intuitively reflect the changing areas and influencing boundaries from a macro perspective, and has a relatively accurate prediction on the trend of yellowfin tuna catch in the Pacific Ocean.
Numerical Investigation of Solitary Wave Attenuation by a Vertical Plate-Type Flexible Breakwater Constructed Using Hyperelastic Neo-Hookean Material
Authors: Sun Weiyi , Nakamura Tomoaki , Cho Yonghwan , Mizutani Norimi
Published: June 2024

This study conducted numerical investigations on solitary wave attenuation by a vertical plate-type flexible breakwater constructed using hyperelastic neo-Hookean material. The wave attenuation performance and elastic behaviors of the flexible breakwater were discussed systematically by considering the effects of three prominent factors: mass coefficient, stiffness coefficient, and Poisson’s ratio. It is indicated that more compressible and flexible materials are beneficial for enhancing efficiency in mitigating solitary wave energy and protecting the structure from damage. In addition, the performance of the hyperelastic neo-Hookean material model was compared with that of a linear elastic isotropic material model coupled with linear and nonlinear geometry analysis (LGEOM and NLGEOM) by evaluating several key targets: wave reflection coefficient, transmission coefficient, horizontal tip displacement, and wave load. Our findings revealed that the hyperelastic neo-Hookean material model showed almost the same predictions as the linear elastic isotropic material model with NLGEOM, but significantly diverged from that with LGEOM. The linear elastic isotropic material model with LGEOM cannot capture the nonlinear variations in structural geometry and stress–strain relationship, resulting in the underestimation and overestimation of horizontal tip displacement under moderate and extreme wave loads, respectively. Moreover, it underestimates the damage inflicted by solitary waves due to inaccurately predicted wave reflection and transmission.
The Diversity and Floristic Analysis of Rust Diseases in the Sanjiangyuan Forest Plants
Authors: Xu Qi , Bai Luchao
Published: June 2024

Between 2020 and 2023, rust fungus specimens were collected from the primary forested regions of the Sanjiangyuan area in Qinghai Province, resulting in over 300 samples. A taxonomic and phylogenetic study of the rust fungi from these forests was conducted using morphological and molecular biological techniques. The investigation identified rust fungi from 7 families, 12 genera, 56 species and varieties, including 10 new host records, 1 new record for China, and 2 novel species. The host plants involved belonged to 26 families, 48 genera, and 78 species. Pucciniaceae and Coleosporiaceae were the dominant families, with the genera Puccinia, Melampsora, and Gymnosporangium being prevalent. The rust fungi in the Sanjiangyuan forests showed a biogeographical affinity with the North Temperate Zone. Floristic comparisons revealed a higher similarity with rust fungi from Inner Mongolia, Gansu, and Tibet and a lower similarity with those from Hainan. An analysis of the life forms of rust fungus host plants indicated that herbaceous plants were the most common, followed by shrubs and trees. In different regions of Sanjiangyuan, rust fungi were found as follows: Golog Prefecture with 6 families, 9 genera, and 28 species; Yushu Prefecture with 5 families, 8 genera, and 31 species; Huangnan Prefecture with 5 families, 9 genera, and 26 species; and Hainan Prefecture with 4 families, 5 genera, and 10 species. The families Pucciniaceae, Melampsoraceae, and Coleosporiaceae were common across all four regions. Moreover, the families Rosaceae, Asteraceae, Ranunculaceae, Salicaceae, and Caprifoliaceae were shared among the host plants in these regions.
“News Desertification” in Europe: Highlighting Correlations for Future Research
Authors: Kermer Jan , Reviglio Urbano , Blagojev Tijana
Published: June 2024

The Local Media for Democracy (LM4D) project is the first project that explores news deserts in Europe comprehensively. Its final report highlighted challenges and opportunities for local and community media and identified the level of risk of news deserts in the 27 European Union (EU) Member States. As a pilot project, however, its results are still preliminary and open to evaluation, and, thus, they require further discussion and corroboration. Drawing from these results, and applying a multidisciplinary approach, we extrapolated research directions for the study of news deserts in the EU. By cross-analyzing data from the LM4D dataset with other datasets, we provide a preliminary analysis of three promising lines of research: (1) the relationship between public service media quality and news deserts, (2) news deserts and the spread of disinformation, and (3) social media usage and news desertification. We conclude by pointing out the limitations of this preliminary analysis and opportunities for further research on news deserts in the EU.
Methodological Proposal for Sustainable Territorial Communication—The Case of Castilla y León
Authors: Hernández-Prieto Marina , Pérez-Alaejos María de la Peña
Published: June 2024

The proximity television model adopted by Castilla y León since 1999 is based on promoting territorial identity and closeness to citizens, in line with the principles of identity and proximity in regional television, albeit with the particularity in the Spanish context of being a regional television system managed by a private concessionaire. In response to the current challenges of the television sector, a consultancy project is implemented with a methodological approach based on a SWOT analysis and the participation of multiple actors. This work details the methodology followed during the different phases of this interdisciplinary consultancy work: definition of bases, development of a base document, and concretion of actions, until reaching the elaboration of a detailed guide that establishes strategic objectives and specific actions. This strategic approach translates into a proposal to improve the performance of regional television and its contribution to the economic and social development of the region; however, the difficulty for its effective implementation due to an uncertain political scenario is pointed out.
Canal Heritage Tourism Utilization Models: Experience and Inspirations from the Grand Canal (Beijing Section)
Authors: Zhang Shuying , Yu Wenting
Published: June 2024

Tourism utilization is an important means for revitalizing canal heritage. Exploring the utilization models of canal heritage tourism is the prerequisite to realizing the sustainable development of canals. Based on previous studies, this study defines two important models of community concentration and corridor expansion. Through an empirical comparison of the Old City section and the Tongzhou section along the Grand Canal in Beijing, this study systematically explores the theoretical mechanism and law of the differences between the two models of tourism utilization. Utilizing a collection of the literature, field investigations, and in-depth interview data and using the theory of a figure-ground relationship, this study discusses the characteristics of the two models of canal heritage tourism utilization and the reasons for their differences. The results reveal that community-concentrated and corridor-expanded models show great differences in the development process, heritage and tourism resources, and spatial organization. The formation of the differences results from location, economic, cultural, ecological, and accidental factors as the driving forces and the continuous self-adjustment of the areas along the canal. This study provides theoretical constructs and policy recommendations for optimization and sustainable canal heritage development.
Analysis of Spatial and Temporal Pattern Evolution and Decoupling Relationships of Land Use Functions Based on Ecological Protection and High-Quality Development: A Case Study of the Yellow River Basin, China
Authors: Du Hanwen , Wang Zhanqi , Li Haiyang , Zhang Chen
Published: June 2024

With rapid industrialization and urbanization, the contradiction between the human exploitation of land production and living functions and natural ecosystem service functions has intensified. The issues of how to coordinate the exploitation and conservation functions of land and guide the rational distribution of human activities have become important for global sustainable development, especially considering the realization that multifunctional land use is an effective way to relieve land pressure and improve land use efficiency, that land multifunction has significant spatio-temporal heterogeneity, and that there is a mutual promotion and stress relationship between multifunctional land use. However, few existing studies have discussed the decoupling relationship among land use functions. In this study, a system of 10 sub-functions and 25 indicators was established based on the production function (PDF), living function (LVF), and ecological function (ELF) for 59 cities in the Yellow River Basin (YRB). There are both subjective and objective procedures employed to determine the weights, while an exploratory spatial data analysis is used to analyze the time-based and territorial changes in various functions of land use in the study area from 2000 to 2020. The decoupling relationship between the three functions is detected utilizing the theoretical foundation of the decoupling analysis. The results show that land use is multifunctional, LUFs develop unevenly, and their spatial distribution varies substantially. The results of the decoupling analysis demonstrate that the predominant types of correlations among the land use ELF and PDF and LVF over the research period are strong decoupling and strong negative decoupling correlations, with the former being a dilemma and the latter being a sustainable type of development.
Spatial Disparity of Neighborhood Food Environment by Socioeconomic Status: Application of Urban Network Analysis
Authors: Seong Taekyung , Lee Sugie
Published: June 2024

There is a large amount of academic research to date on food environments that has been widely conducted in Western countries. This is due to the high population density of Asian megacities, the advancement of public transportation systems, and the relatively low dependence on private vehicles, which has led to a lack of attention, relatively, to the surrounding food environments. Thus, this study aimed to analyze food environments at the neighborhood level by considering demographics, households, land use, and physical environmental factors in Seoul, South Korea. We employed urban network analysis methods to assess food access and availability in various neighborhoods. The primary findings indicate that Seoul’s food environment is significantly related to the elderly population, aging infrastructure, and proximity to public transportation. In particular, a higher concentration of elderly residents negatively affects food availability and access, irrespective of income level, potentially exacerbating social inequality based on income. Our findings identify various factors that contribute to spatial disparities in local food environments and provide policy implications to mitigate inequalities in neighborhood food environments.
Economics of Peatland Ecosystem Services: A Study of Use and Non-Use Values and People Interplays in Sumatra, Indonesia
Authors: Yunus Mohammad , Pagdee Adcharaporn , Baral Himlal
Published: June 2024

Peatlands play an important role in the global environment and the well-being of humans by providing valuable ecosystem services. Yet, anthropogenic activities pose significant hazards for peatland management, including low levels of community participation due to lack of awareness and financial incentives. Understanding the social–cultural and economic value of these ecosystems will raise awareness to protect these important ecosystems. Here, we estimated a total economic value (TEV) of peatland ecosystem services and examined relationships between the TEV and landscape characteristics in Riau province, Indonesia. A questionnaire was used to investigate household socioeconomics, perception of peatland importance, peatland product collection, and willingness to pay for habitat and biodiversity protection from May to June 2023. A total of 200 household individuals (92% confidence) in five villages across distinct landscapes in the Sungai Kiyap-Sungai Kampar Kiri Peatland Hydrological Unit participated in the survey. The respondents obtained numerous advantages from the peatlands with an estimated TEV of USD 3174 per household per year (about 1.3 times their annual income). Approximately 81% showed a use value, especially food provisioning from fish and soil fertility. To a lesser extent, non-use values included a habitat for endemic and endangered species, biodiversity conservation for future generations, and community bonds with sacred forests. The landscape characteristics, illustrating habitat types, biophysical conditions, and property rights regimes, interplay with the relative benefits derived from the peatlands. Proximity to secondary peat swamp forests and riparian zones, especially within protected areas, enhanced economic value. Protected area co-management is essential to balance peatland conservation with sustainable livelihoods. Primary forests need restrictive protection. Meanwhile, buffer zone designation and agroforestry practices, especially in the peatland–farm interface, reduce land use tensions and promote local stewardship. This study can be used as a reference by planners and policymakers to recognize factors that promote effective peatland management, especially those that balance ecosystem protection and livelihood maintenance.
Reconciling the Mismatch: Creating a Regenerative Framework for Regional Planning
Authors: Roggema Rob , Junco Rodrigo
Published: June 2024

The world is urbanizing rapidly, and many urbanized regions deplete and degrade their environment. The additional polycrisis of climate change, biodiversity loss, epidemics, food insecurity, and reduced water, air, and soil quality asks for a transformational vision for the design and planning of these urban regions. Current planning practices are not able to respond to the complexity of the problems associated with the polycrisis. At the same time, regenerative thinking has not yet been practical enough to be accepted into spatial planning practices and create regenerative regions that can respond to the global polycrisis. This mismatch reinforces the status quo of well-thought-through regenerative frameworks on the one hand and ongoing spatial planning in urban regions on the other. The aim of this study is to create a regenerative framework for regional planning. A range of regenerative frameworks have been analyzed and integrated into one ‘framework of frameworks’, highlighting clusters of attributes describing the ‘what’, ‘when’, ‘how’, and ‘who’ of what needs to happen to create a regenerative world. On the other hand, expert judgement of planning practices in nine urban regions around the world provided insight about the priorities for regional regenerative development. This clarified the ‘what’ and ‘where’ of different aspects of urban planning in the region. Bringing the theoretical frameworks and practical understanding of urban planning together, the regenerative framework for regional planning provides a practical approach for navigating the complexities of creating a regenerative region. It starts by backtracking to the 1st generation city (of first settlements and indigenous understanding of the land). When this equilibrium is found, it is used to create a vision for the 3rd generation city (in which regenerative potentials are fully used). The comparison with the 2nd generation city (a current anthropogenic industrial city) clarifies what must change, how this change can be achieved, and who the agents of change are to make it happen.