bluuebunny/crossref_metadata_2025 huggingface dataset:
Genomic and Micro-Evolutionary Features of Mammalian 2 orthobornavirus (Variegated Squirrel Bornavirus 1, VSBV-1)
Authors: Cadar Dániel , Schmidt-Chanasit Jonas , Tappe Dennis
Published: May 2021

Mammalian 2 orthobornavirus (VSBV-1) is an emerging zoonotic pathogen discovered in several exotic squirrel species and associated with fatal human encephalitis. The dynamics of VSBV-1 spread and evolution in its presumed natural hosts are unknown. Here, we present the phylogeny, micro-evolution, cross-species transmission and spread of VSBV-1 at a temporal and spatial resolution within the limits of animal husbandry. The results showed that VSBV-1 can be classified into six distinct groups and that the most recent common ancestor of the known German strains emerged at least 20 years ago. We here demonstrate that the genetic diversity of the VSBV-1 groups is shaped primarily by in situ evolution and most of the amino acid changes are deleterious polymorphisms removed by purifying selection. Evidence of adaptive evolution has been found in the G and L genes which might have an influence on transmission fitness. Furthermore, there was also evidence for some form of adaptive changes in the glycoprotein which suggests that many sites might be subjected to positive pressure evolving under episodic directional selection, indicating past occurrence of positive selection. Host switching events were detected as dominant evolutionary mechanisms driving the virus-host associations. Virus spread by animal trade followed by subsequent local micro-evolution in zoos and holdings is responsible for diversifying strains. Time-resolved phylogeny indicated that Prevost’s squirrels might be the original squirrel species carrying and seeding the virus in Germany. This study provides the first insight into the ecology and micro-evolutionary dynamics of this novel viral pathogen in the captive exotic squirrel population under artificial ecological conditions (zoos and animal husbandry) and co-housing of different squirrel species.
Cutting Resistance Laboratory Testing Methodology for Underwater Coal Mining
Authors: Čebašek Vladimir , Rupar Veljko , Đenadić Stevan , Miletić Filip
Published: May 2021

The bucket-wheel dredge “Kovin I” for underwater coal mining with bucket-wheel type UCW-450 has been in operation for over 20 years. Based on analyzing the bucket-wheel dredger performance, productivity, maintenance costs, and reliability, a rational decision was made: to rehabilitate the most essential parts of the dredge, including the bucket wheel and the gearbox. However, the selection and construction of the excavator parts were performed on the ground of available laboratory data for digging resistance. The data itself was determined by the testing methodology that did not include the influence of surrounding water pressure at a certain depth of mining. According to the previous findings, it was necessary to develop a specific research and testing program that would involve appropriate laboratory testing of the geomechanical parameters. These were to represent the influence of hydrostatic water pressure on the working environment—coal. Nevertheless, geomechanical laboratory research tests were initially modified to provide reliable data of cutting resistance, especially in the water under different hydrostatic pressures, fully simulating the “in situ” working conditions of mining, i.e., cutting.
Synthesis and Biological Evaluation of Spirocyclic Chromane Derivatives as a Potential Treatment of Prostate Cancer
Authors: Feng Li , Yu Shujia , Wang Hai , Yang Shengwei , Li Xue , Dai Hongjuan , Zhao Liwen , Jiang Cheng , Wang Yazhou
Published: May 2021

As a significant co-activator involved in cell cycle and cell growth, differentiation and development, p300/CBP has shown extraordinary potential target in cancer therapy. Herein we designed new compounds from the lead compound A-485 based on molecular dynamic simulations. A series of new spirocyclic chroman derivatives was prepared, characterized and proven to be a potential treatment of prostate cancer. The most potent compound B16 inhibited the proliferation of enzalutamide-resistant 22Rv1 cells with an IC50 value of 96 nM. Furthermore, compounds B16–P2 displayed favorable overall pharmacokinetic profiles, and better tumor growth inhibition than A-485 in an in vivo xenograft model.
Synthesis of Novel Halogenated Heterocycles Based on o-Phenylenediamine and Their Interactions with the Catalytic Subunit of Protein Kinase CK2
Authors: Winiewska-Szajewska Maria , Maciejewska Agnieszka Monika , Speina Elżbieta , Poznański Jarosław , Paprocki Daniel
Published: May 2021

Protein kinase CK2 is a highly pleiotropic protein kinase capable of phosphorylating hundreds of protein substrates. It is involved in numerous cellular functions, including cell viability, apoptosis, cell proliferation and survival, angiogenesis, or ER-stress response. As CK2 activity is found perturbed in many pathological states, including cancers, it becomes an attractive target for the pharma. A large number of low-mass ATP-competitive inhibitors have already been developed, the majority of them halogenated. We tested the binding of six series of halogenated heterocyclic ligands derived from the commercially available 4,5-dihalo-benzene-1,2-diamines. These ligand series were selected to enable the separation of the scaffold effect from the hydrophobic interactions attributed directly to the presence of halogen atoms. In silico molecular docking was initially applied to test the capability of each ligand for binding at the ATP-binding site of CK2. HPLC-derived ligand hydrophobicity data are compared with the binding affinity assessed by low-volume differential scanning fluorimetry (nanoDSF). We identified three promising ligand scaffolds, two of which have not yet been described as CK2 inhibitors but may lead to potent CK2 kinase inhibitors. The inhibitory activity against CK2α and toxicity against four reference cell lines have been determined for eight compounds identified as the most promising in nanoDSF assay.
Detection of Zoonotic Cryptosporidium ubiquitum in Alpine Wild Ruminants
Authors: Trogu Tiziana , Formenti Nicoletta , Marangi Marianna , Viganò Roberto , Bionda Radames , Giangaspero Annunziata , Lanfranchi Paolo , Ferrari Nicola
Published: May 2021

Cryptosporidium is a widespread apicomplexan protozoan of major zoonotic importance, characterized by a wide host range, and with relevant economic implications and potential negative effects on livestock and wildlife population dynamics. Considering the recent strong demographic increase of alpine ungulates, in this study, carried out in the Italian Northwestern Alps, we investigated the occurrence of Cryptosporidium spp. in these species and their potential involvement in environmental contamination with Cryptosporidium spp. oocysts. The immune-enzymatic approach revealed a Cryptosporidium prevalence of 1.7% (5/293), 0.5% (1/196) and 3.4% (4/119) in alpine chamois (Rupicapra rupicapra), red deer (Cervus elaphus) and roe deer (Capreolus capreolus), respectively. Positive samples were subjected to polymerase chain reaction (PCR) amplification for the COWP and gp60 genes. The presence of Cryptosporidium was confirmed in one chamois and four roe deer. Sequences obtained clustered within Cryptosporidium ubiquitum, currently recognized as an emerging zoonotic species. This finding represents the first detection of zoonotic C. ubiquitum in chamois and in the Alpine environment. Despite the low observed prevalences, environmental contamination by oocysts could play a role as a potential source of infections for humans and livestock.
Doctrine of the Ciphers Intercursions among Zeropoint-Utopia-Core
Authors: Zeilinger Doris
Published: May 2021

The Blochian concept of cipher is discussed in some detail with a view to possible developments in the modern philosophy of nature. Parallels and differences are listed as to the Idealistic tradition in Germany preceding Bloch’s approach. It is found that within the framework of a strict process philosophy of the Blochian type, life forms and human (reflexive) life in particular show up as systemic parts of a nature that is projecting itself towards what has not yet become, hence a strong similarity to Schelling’s approach.
Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
Authors: Li Jinqiu , Duan Yu , Han Zhaolan , Shang Xiaowen , Zhang Kexin , Zou Zhongwei , Ma Yuanchun , Li Fang , Fang Wanping , Zhu Xujun
Published: May 2021

The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in Camellia sinensis. In this study, 11 NRAMP genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 CsNRAMP genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, CsNRAMP3, CsNRAMP4, and CsNRAMP5, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of CsNRAMPs in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that CsNRAMPs were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the CsNRAMP family could provide primary information for further studies on the functional roles of CsNRAMPs in divalent metal transportation in tea plants.
The Volatile Phytochemistry of Seven Native American Aromatic Medicinal Plants
Authors: Lawson Sims K. , Satyal Prabodh , Setzer William N.
Published: May 2021

As part of our evaluation of essential oils derived from Native American medicinal plants, we have obtained the essential oils of Agastache foeniculum (Pursch) Kuntze (Lamiaceae), Gaultheria procumbens L. (Ericaceae), Heliopsis helianthoides (L.) Sweet (Asteraceae), Liatris spicata (L.) Willd. (Asteraceae), Pycnanthemum incanum (L.) Michx. (Lamiaceae), Smallanthus uvedalia (L.) Mack. ex Mack. (Asteraceae), and Verbena hastata L. (Verbenaceae) by hydrodistillation. The essential oils were analyzed by gas chromatographic techniques. The essential oil of A. foeniculum was dominated by estragole (88–93%), while methyl salicylate (91%) dominated the G. procumbens essential oil. Germacrene D was the major component in H. helianthoides (42%) and L. spicata (24%). 1,8-Cineole (31%) and α-terpineol (17%) were the main compounds in P. incanum essential oil. The essential oil of S. uvedalia showed α-pinene (24%), perillene (15%), and β-caryophyllene (17%) as major components. Verbena hastata essential oil was rich in 1-octen-3-ol (up to 29%) and palmitic acid (up to 22%). Four of these essential oils, H. helianthoides, L. spicata, P. incanum, and V. hastata, are reported for the first time. Additionally, the enantiomeric distributions of several terpenoid components have been determined.
Special Issue on Planning, Designing and Managing Decentralized Drinking Water Supply System—Editorial
Authors: Noubactep Chicgoua
Published: May 2021

The growing demands for affordable and applicable technologies for decentralized safe drinking water provision have instigated technical innovations in the water filtration industry. Adsorptive filtration appears to be the most affordable, resilient, and socially acceptable solution for households and small communities worldwide. However, water filtration devices have not yet been widely implemented due to lack of awareness for the efficiency of such systems using locally available materials. Water filtration has the potential to secure universal access to safe drinking water by 2030. This special issue has elucidated the applicability, benefits, constraints, effectiveness, and limitations of metallic iron as filter material for safe drinking water provision. Tools to make rainwater a primary water source are also presented together with ways to transform existing centralized water management systems into decentralized ones (sectorization). The knowledge is applicable to a wide variety of situations on a global scale.
Curcumin and Freshwater Clam Extracts Alleviate the Progression of Osteoarthritis by Reducing Synovial Inflammation and Allowing Cartilage Regeneration
Authors: Ke Chiao-Hsu , Hsu Chia-Hui , Lin Yu-Ling , Huang Wei-Hsiang , Weng Hsin-Pei , Ke Yi-Tzu , Lin Chen-Si
Published: May 2021

Osteoarthritis (OA) is a common degenerative disorder and is accompanied by numerous pain symptoms. With increased age, individuals develop a chronic inflammatory status, and pro-inflammatory cytokines as well as mediators contribute to the progression of OA. However, no desirable remedies have been completely able to inhibit OA progression or safely provide effective symptomatic relief. Natural component extracts or dietary-derived compounds are widely used for anti-inflammatory diseases. Curcumin and freshwater clam extract (FCE) have been proven as functional foods that are able to regulate immune systems. This study demonstrated that curcumin and FCE had synergistic effects on alleviating the progression of OA by assuaging inflammation and repairing the cartilage within the joints. After consumption of curcumin and FCE, the severity of synovitis was quantified by the infrapatellar fat pad inflammation scoring system and the Osteoarthritis Research Society International (OARSI) scoring system. Significant improvement and articular cartilage regeneration were noted. Moreover, once the inflammation within the joints was reduced, the animals redistributed their body weight on the OA-induced hindlimb. In summary, curcumin and FCE possess desirable anti-inflammatory and repair functions, suggesting their potential as alternative remedies in the management of OA or other inflammatory diseases.
Fabrication and Characterisation of Organic EL Devices in the Presence of Cyclodextrin as an Interlayer
Authors: Hara Michihiro , Umeda Takao , Kurata Hiroyuki
Published: May 2021

This study examined glass-based organic electroluminescence in the presence of a cyclodextrin polymer as an interlayer. Glass-based organic electroluminescence was achieved by the deposition of five layers of N,N’-Bis(3-methylphenyl)N,N’-bis(phenyl)-benzidine, cyclodextrin polymer (CDP), tris-(8-hydroxyquinolinato) aluminium LiF and Al on an indium tin oxide-coated glass substrate. The glass-based OEL exhibited green emission owing to the fluorescence of tris-(8-hydroxyquinolinato) aluminium. The highest luminance was 19,620 cd m−2. Moreover, the glass-based organic electroluminescence device showed green emission at 6 V in the curved state because of the inhibited aggregation of the cyclodextrin polymer. All organic molecules are insulating, but except CDP, they are standard molecules in conventional organic electroluminescence devices. In this device, the CDP layer contained pores that could allow conventional organic molecules to enter the pores and affect the organic electroluminescence interface. In particular, self-association was suppressed, efficiency was improved, and light emission was observed without the need for a high voltage. Overall, the glass-based organic electroluminescence device using CDP is an environmentally friendly device with a range of potential energy saving applications.
Energy Recovery of Multiple Charge Sharing Events in Room Temperature Semiconductor Pixel Detectors
Authors: Buttacavoli Antonino , Gerardi Gaetano , Principato Fabio , Mirabello Marcello , Cascio Donato , Raso Giuseppe , Bettelli Manuele , Zappettini Andrea , Seller Paul , Veale Matthew C. , Abbene Leonardo
Published: May 2021

Multiple coincidence events from charge-sharing and fluorescent cross-talk are typical drawbacks in room-temperature semiconductor pixel detectors. The mitigation of these distortions in the measured energy spectra, using charge-sharing discrimination (CSD) and charge-sharing addition (CSA) techniques, is always a trade-off between counting efficiency and energy resolution. The energy recovery of multiple coincidence events is still challenging due to the presence of charge losses after CSA. In this work, we will present original techniques able to correct charge losses after CSA even when multiple pixels are involved. Sub-millimeter cadmium–zinc–telluride (CdZnTe or CZT) pixel detectors were investigated with both uncollimated radiation sources and collimated synchrotron X rays, at energies below and above the K-shell absorption energy of the CZT material. These activities are in the framework of an international collaboration on the development of energy-resolved photon counting (ERPC) systems for spectroscopic X-ray imaging up to 150 keV.
Design and Analysis of Broadband LiNbO3 Optical Waveguide Electric Field Sensor with Tapered Antenna
Authors: Lu Haiying , Li Yingna , Zhang Jiahong
Published: May 2021

The three-dimensional (3D) simulation model of a lithium niobate (LiNbO3, LN) optical waveguide (OWG) electric field sensor has been established by using the full-wave electromagnetic simulation software. The influences of the LN substrate and the packaging material on the resonance frequency of the integrated OWG electric field sensor have been simulated and analyzed. The simulation results show that the thickness of the LN substrate has a great influence on the resonant frequency of the sensor (≈33.4%). A sensor with a substrate thickness of 1 mm has been designed, fabricated, and experimentally investigated. Experimental results indicate that the measured resonance frequency is 7.5 GHz, which nearly coincides with the simulation results. Moreover, the sensor can be used for the measurement of the nanosecond electromagnetic impulse (NEMP) in the time domain from 1.29 kV/m to 100.97 kV/m.
Flexible and Transparent Polymer-Based Optical Humidity Sensor
Authors: Lazarova Katerina , Bozhilova Silvia , Ivanova Sijka , Christova Darinka , Babeva Tsvetanka
Published: May 2021

Thin spin-coated polymer films of amphiphilic copolymer obtained by partial acetalization of poly (vinyl alcohol) are used as humidity-sensitive media. They are deposited on polymer substrate (PET) in order to obtain a flexible humidity sensor. Pre-metallization of substrate is implemented for increasing the optical contrast of the sensor, thus improving the sensitivity. The morphology of the sensors is studied by surface profiling, while the transparency of the sensor is controlled by transmittance measurements. The sensing behavior is evaluated through monitoring of transmittance values at different levels of relative humidity gradually changing in the range 5–95% and the influence of up to 1000 bending deformations is estimated by determining the hysteresis and sensitivity of the flexible sensor after each set of deformations. The successful development of a flexible sensor for optical monitoring of humidity in a wide humidity range is demonstrated and discussed.
3D-Printed Load Cell Using Nanocarbon Composite Strain Sensor
Authors: Joung Kwan-Young , Kim Sung-Yong , Kang Inpil , Cho Sung-Ho
Published: May 2021

The development of a 3D-Printed Load Cell (PLC) was studied using a nanocarbon composite strain sensor (NCSS) and a 3D printing process. The miniature load cell was fabricated using a low-cost LCD-based 3D printer with UV resin. The NCSS composed of 0.5 wt% MWCNT/epoxy was used to create the flexure of PLC. PLC performance was evaluated under a rated load range; its output was equal to the common value of 2 mV/V. The performance was also evaluated after a calibration in terms of non-linearity, repeatability, and hysteresis, with final results of 2.12%, 1.60%, and 4.42%, respectively. Creep and creep recovery were found to be 1.68 (%FS) and 4.16 (%FS). The relative inferiorities of PLC seem to originate from the inherent hyper-elastic characteristics of polymer sensors. The 3D PLC developed may be a promising solution for the OEM/design-in load cell market and may also result in the development of a novel 3D-printed sensor.
Ultrafast Fiber Lasers with Low-Dimensional Saturable Absorbers: Status and Prospects
Authors: Debnath Pulak Chandra , Yeom Dong-Il
Published: May 2021

Wide-spectral saturable absorption (SA) in low-dimensional (LD) nanomaterials such as zero-, one-, and two-dimensional materials has been proven experimentally with outstanding results, including low saturation intensity, deep modulation depth, and fast carrier recovery time. LD nanomaterials can therefore be used as SAs for mode-locking or Q-switching to generate ultrafast fiber laser pulses with a high repetition rate and short duration in the visible, near-infrared, and mid-infrared wavelength regions. Here, we review the recent development of emerging LD nanomaterials as SAs for ultrafast mode-locked fiber laser applications in different dispersion regimes such as anomalous and normal dispersion regimes of the laser cavity operating in the near-infrared region, especially at ~1550 nm. The preparation methods, nonlinear optical properties of LD SAs, and various integration schemes for incorporating LD SAs into fiber laser systems are introduced. In addition to these, externally (electrically or optically) controlled pulsed fiber laser behavior and other characteristics of various LD SAs are summarized. Finally, the perspectives and challenges facing LD SA-based mode-locked ultrafast fiber lasers are highlighted.
Measurement of Stress Waves Propagation in Percussive Drilling
Authors: Scaccabarozzi Diego , Saggin Bortolino
Published: May 2021

This work describes the results of a test campaign aimed to measure the propagation of longitudinal, torsional, and flexural stress waves on a drill bit during percussive rock drilling. Although the stress wave propagation during percussive drilling has been extensively modeled and studied in the literature, its experimental characterization is poorly documented and generally limited to the detection of the longitudinal stress waves. The activity was performed under continuous drilling while varying three parameters, the type of concrete, the operator feeding force, and the drilling hammer rotational speed. It was found that axial stress wave frequencies and spectral amplitudes depend on the investigated parameters. Moreover, a relevant coupling between axial and torsional vibrations was evidenced, while negligible contribution was found from the bending modes. A finite element model of the drill bit and percussive element was developed to simulate the impact and the coupling between axial and torsional vibrations. A strong correlation was found between computed and measured axial stress spectra, but additional studies are required to achieve a satisfactory agreement between the measured and the simulated torque vibrations.
Feature Extraction Using Sparse Kernel Non-Negative Matrix Factorization for Rolling Element Bearing Diagnosis
Authors: Liang Lin , Ding Xingyun , Liu Fei , Chen Yuanming , Wen Haobin
Published: May 2021

For early fault detection of a bearing, the localized defect generally brings a complex vibration signal, so it is difficult to detect the periodic transient characteristics from the signal spectrum using conventional bearing fault diagnosis methods. Therefore, many matrix analysis technologies, such as singular value decomposition (SVD) and reweighted SVD (RSVD), were proposed recently to solve this problem. However, such technologies also face failure in bearing fault detection due to the poor interpretability of the obtained eigenvector. Non-negative Matrix Factorization (NMF), as a part-based representation algorithm, can extract low-rank basis spaces with natural sparsity from the time–frequency representation. It performs excellent interpretability of the factor matrices due to its non-negative constraints. By this virtue, NMF can extract the fault feature by separating the frequency bands of resonance regions from the amplitude spectrogram automatically. In this paper, a new feature extraction method based on sparse kernel NMF (KNMF) was proposed to extract the fault features from the amplitude spectrogram in greater depth. By decomposing the amplitude spectrogram using the kernel-based NMF model with L1 regularization, sparser spectral bases can be obtained. Using KNMF with the linear kernel function, the time–frequency distribution of the vibration signal can be decomposed into a subspace with different frequency bands. Thus, we can extract the fault features, a series of periodic impulses, from the decomposed subspace according to the sparse frequency bands in the spectral bases. As a result, the proposed method shows a very high performance in extracting fault features, which is verified by experimental investigations and benchmarked by the Fast Kurtogram, SVD and NMF-based methods.
Differences in Physiological Reactions Due to a Competitive Rehabilitation Game Modality
Authors: Catalán José M. , García-Pérez José V. , Blanco Andrea , Martínez David , Lledó Luis D. , García-Aracil Nicolás
Published: May 2021

Interpersonal rehabilitation games, compared to single-player games, enhance motivation and intensity level. Usually, it is complicated to restrict the use of the system to pairs of impaired patients who have a similar skill level. Thus, such games must be dynamically adapted. Difficulty-adaptation algorithms are usually based only on performance parameters. In this way, the patient’s condition cannot be considered when adapting the game. Introducing physiological reactions could help to improve decision-making. However, it is difficult to control how social interaction influences physiological reactions, making it difficult to interpret physiological responses. This article aimed to explore the changes in physiological responses due to the social interaction of a competitive game modality. This pilot study involved ten unimpaired participants (five pairs). We defined different therapy sessions: (i) a session without a competitor; (ii) two sessions with a virtual competitor with different difficulty levels; (iii) a competitive game. Results showed a difference in the physiological response in the competitive mode concerning single-player mode only due to the interpersonal game modality. In addition, feedback from participants suggested that it was necessary to keep a certain difficulty level to make the activity more challenging, and therefore be more engaging and rewarding.
Low-Latency Haptic Open Glove for Immersive Virtual Reality Interaction
Authors: Sim Donghyun , Baek Yoonchul , Cho Minjeong , Park Sunghoon , Sagar A. S. M. Sharifuzzaman , Kim Hyung Seok
Published: May 2021

Recent advancements in telecommunications and the tactile Internet have paved the way for studying human senses through haptic technology. Haptic technology enables tactile sensations and control using virtual reality (VR) over a network. Researchers are developing various haptic devices to allow for real-time tactile sensation, which can be used in various industries, telesurgery, and other mission-critical operations. One of the main criteria of such devices is extremely low latency, as low as 1 ms. Although researchers are attempting to develop haptic devices with low latency, there remains a need to improve latency and robustness to hand sizes. In this paper, a low-latency haptic open glove (LLHOG) based on a rotary position sensor and min-max scaling (MMS) filter is proposed to realize immersive VR interaction. The proposed device detects finger flexion/extension and adduction/abduction motions using two position sensors located in the metacarpophalangeal (MCP) joint. The sensor data are processed using an MMS filter to enable low latency and ensure high accuracy. Moreover, the MMS filter is used to process object handling control data to enable hand motion-tracking. Its performance is evaluated in terms of accuracy, latency, and robustness to finger length variations. We achieved a very low processing delay of 145.37 μs per finger and overall hand motion-tracking latency of 4 ms. Moreover, we tested the proposed glove with 10 subjects and achieved an average mean absolute error (MAE) of 3.091∘ for flexion/extension, and 2.068∘ for adduction/abduction. The proposed method is therefore superior to the existing methods in terms of the above factors for immersive VR interaction.
The Human Family—Its Evolutionary Context and Diversity
Authors: Kramer Karen L.
Published: May 2021

The family defines many aspects of our daily lives, and expresses a wide array of forms across individuals, cultures, ecologies and time. While the nuclear family is the norm today in developed economies, it is the exception in most other historic and cultural contexts. Yet, many aspects of how humans form the economic and reproductive groups that we recognize as families are distinct to our species. This review pursues three goals: to overview the evolutionary context in which the human family developed, to expand the conventional view of the nuclear family as the ‘traditional family’, and to provide an alternative to patrifocal explanations for family formation. To do so, first those traits that distinguish the human family are reviewed with an emphasis on the key contributions that behavioral ecology has made toward understanding dynamics within and between families, including life history, kin selection, reciprocity and conflict theoretical frameworks. An overview is then given of several seminal debates about how the family took shape, with an eye toward a more nuanced view of male parental care as the basis for family formation, and what cooperative breeding has to offer as an alternative perspective.
Territorial and Human Geography Challenges: How Can Smart Villages Support Rural Development and Population Inclusion?
Authors: Anastasiou Evgenia , Manika Stella , Ragazou Konstantina , Katsios Ioannis
Published: May 2021

Rural areas are significantly affected by spatial vulnerability, the digital gap, depopulation, and population ageing. Marginalized populations are seeking collective well-being, social inclusion, and local development in smart villages, an increasingly important area of interest for scholars and practitioners as well as rural areas and communities. This article attempts to highlight the dominant trends in smart villages planning and depict the characteristics of Greek rural areas and populations alongside the implemented localized smart actions. To achieve this aim, the research utilized the existing literature through bibliometric analysis by extracting data from the Web of Science database. Building upon the bibliometrics, the research focused on identifying localized implemented interventions in the Greek rural areas. The results suggest that innovation, knowledge, growth, and management appear to impact rural smart planning, while the limited interventions of smart villages in Greece focus on social innovation and local development. The study argues that in Greece, a single holistic smart villages model cannot be proposed, due to the country’s geographical and demographical variability. The proposed trends, though, can be implemented locally to encourage rural development and population inclusion; therefore it is recommended to increase local stakeholders’ awareness and active engagement.
Physicochemical Characterisation and the Prospects of Biofuel Production from Rubberwood Sawdust and Sewage Sludge
Authors: Ali Liaqat , Ahmed Baloch Khurshid , Palamanit Arkom , Raza Shan Ali , Laohaprapanon Sawanya , Techato Kuaanan
Published: May 2021

This study aims to evaluate the physicochemical properties of rubberwood sawdust (RWS) and sewage sludge (SS) for producing biofuel or liquid products via pyrolysis and co-pyrolysis. The chemical and thermal properties of both samples were observed to have superior bioenergy production capabilities. RWS and SS had significantly different physicochemical properties, such as particle-size distribution, bulk density, ultimate and proximate analysis, lignocellulose composition, thermal-degradation behaviour, and major and minor elements. The composition of extractives was found to only marginally affect the end product. Carbon and hydrogen content, the two main elements for biofuel enhancement, were found to correlate with the organic components of both RWS (48.49, 7.15 wt.%) and SS (32.29, 4.06 wt.%). SS had a higher elemental composition of iron, calcium, and potassium than RWS. Both samples had a higher heating value of 13.98 to 21.01 MJ/kg and a lower heating value of 11.65 to 17.66 MJ/kg, a lesser energy potential than that of fossil fuels. The findings from these blends are relatively moderate due to the related lignocellulosic potential composition. The novel contribution of this research was to optimize the use of local waste materials as a new raw material for biofuel production that could serve as a sustainable fuel source.
Behavior Patterns, Energy Consumption and Comfort during COVID-19 Lockdown Related to Home Features, Socioeconomic Factors and Energy Poverty in Madrid
Authors: Cuerdo-Vilches Teresa , Navas-Martín Miguel Ángel , Oteiza Ignacio
Published: May 2021

During spring 2020, the world was shocked at the imminent global spread of SARS-CoV-2, resorting to measures such as domestic confinement. This meant the reconfiguration of life in an unusual space; the home. However, not all households experienced it in the same way; many of them were vulnerable. A general increase in energy consumption and discomfort in many cases, led these families to suffer the ravages of confinement. This study analyzes the energy and comfort situation for the Madrid (Spain) population, according to the configuration of the homes, the characteristics of the dwellings, the vulnerability index by district, and energy poverty (measured with the 10% threshold of energy expenditure of home incomes). The results show a greater exposure, in confinement, of vulnerable and energy-poor households to scenarios of discomfort in the home, to which they could not respond, while energy consumption inevitably increased. Driven by need, energy-poor homes applied certain saving strategies, mainly resorting to thermal adaptation with clothing. This study shows the risk these households experienced in the face of an extreme situation, and invites reflection on preventive and containment measures that aim to avoid harming the disadvantaged in the future; harm that would also entail serious consequences on the health of their cohabitants.
Role of Governance in Debt-Growth Relationship: Evidence from Panel Data Estimations
Authors: Abbas Qamar , Junqing Li , Ramzan Muhammad , Fatima Sumbal
Published: May 2021

This paper provides an empirical analysis of the relationship between debt and national output mediated by a measure of the quality of state governance. Using WGIs dataset of 106 countries for the period 1996–2015, the paper analyzes the mediated effect of governance on debt-growth relationship. For this purpose, we use the fixed effect (LSDV) and system GMM estimation technique in order to overcome the possible problem of endogeneity. Results show the non-linear pattern between public debt and economic growth via governance. Although, public debt has negative impact on economic growth, but the results are statistically positive and significant when public debt is interacted with governance, which confirms that governance is a channel by which public debt influences economic growth. Moreover, we calculate the threshold of governance which shows that the public debt has positive impact on economic growth when the governance level is higher than the threshold and adversely affects the economic growth in the case of low level of governance than threshold. Evidence from this study reveals the fact that governance plays a mediating role in debt-growth relationship as there is a pattern of complementarity between public debt and governance: the higher the level of governance, the lesser the adverse effect of public debt on economic growth.
Omni-Channel Intensity and Shopping Value as Key Drivers of Customer Satisfaction and Loyalty
Authors: Cotarelo Mitxel , Fayos Teresa , Calderón Haydeé , Mollá Alejandro
Published: May 2021

Omni-channel retailing consists of the complete alignment of the different channels and touchpoints that generate a seamless experience for consumers, allowing them to move freely through all channels. The consumer’s perception of a seamless and consistent omni-channel experience is called intensity. This study reveals that this intensity in the shopping experience can offer value to consumers, and that intensity and shopping value also influence satisfaction and loyalty. We propose a relationship model that had been tested in a quantitative study with PLS, with a representative sample of buyers who had used the Click and Collect system. The main contribution of this study is to the literature on the analysis of omni-channels from the consumer experience perspective, through an in-depth analysis of the concepts of intensity and shopping value, as well as their relationship with satisfaction and loyalty. The results revealed the existence of a positive relationship between intensity and shopping value, and between these two variables and satisfaction and loyalty.
Pharmacological Modulation of Serotonin Levels in Zebrafish Larvae: Lessons for Identifying Environmental Neurotoxicants Targeting the Serotonergic System
Authors: Faria Melissa , Prats Eva , Bellot Marina , Gomez-Canela Cristian , Raldúa Demetrio
Published: May 2021

This study examines the effects of acute pharmacological modulation of the serotonergic system over zebrafish larvae’s cognitive, basic, and defense locomotor behaviors, using a medium to high throughput screening assay. Furthermore, the relationship between behavior, enzyme activity related to neurotransmitter metabolism, neurotransmitter levels, and gene expression was also determined. Modulation of larvae serotonergic system was accomplished by 24 h exposure to single and opposite pharmacodynamics co-exposure to three model psychopharmaceuticals with antagonistic and agonistic serotonin signaling properties: 2.5 mM 4-Chloro-DL-phenylalanine (PCPA) and 5 µM deprenyl and 0.5 µM fluoxetine, respectively. Similar behavioral outcome was observed for deprenyl and fluoxetine, which was reflected as hypolocomotion, decrease in larvae defensive responses, and cognitive impairment. Contrarily, PCPA induced hyperlocomotion and increase in larvae escape response. Deprenyl exposure effects were more pronounced at a lower level of organization than fluoxetine, with complete inhibition of monoamine oxidase (MAO) activity, dramatic increase of 5-HT and dopamine (DA) levels, and downregulation of serotonin synthesis and transporter genes. PCPA showed mainly effects over serotonin and dopamine’s main degradation metabolites. Finally, co-exposure between agonistic and antagonist serotonin signaling drugs reviled full recovery of zebrafish impaired locomotor and defense responses, 5-HT synthesis gene expression, and partial recovery of 5-HT levels. The findings of this study suggest that zebrafish larvae can be highly sensitive and a useful vertebrate model for short-term exposure to serotonin signaling changes.
Toxicological Evaluation of Novel Cyclohexenone Derivative in an Animal Model through Histopathological and Biochemical Techniques
Authors: Kamil Muhammad , Fatima Arifa , Ullah Sami , Ali Gowhar , Khan Rasool , Ismail Naila , Qayum Mughal , Irimie Marius , Dinu Catalina Georgeta , Ahmedah Hanadi Talal , Cocuz Maria Elena
Published: May 2021

Toxicity studies were conducted to provide safety data of potential drug candidates by determining lethal and toxic doses. This study was designed for pre-clinical evaluation of novel cyclohexenone derivative with respect to the acute and sub-acute toxicity along with the diabetogenic potential. Acute and sub-acute toxicity were assessed after intraperitoneal (i.p) injection of the investigational compound through selected doses for 21 days. This was followed by assessment of isolated body organs (liver, kidney, heart and pancreas) via biochemical indicators and histopathological techniques. No signs of toxicity were revealed in the study of acute toxicity. Similarly, a sub-acute toxicity study showed no significant difference in biochemical indicators on 11th and 21st days between treated and control groups. However, in blood urea nitrogen (BUN) and random blood glucose/sugar (RBS) values, significant differences were recorded. Histopathological evaluation of liver, kidney, pancreas and heart tissues revealed mild to severe changes in the form of steatosis, inflammation, fibrosis, necrosis and myofibrillary damages on 11th and 21st days of treatment. In conclusion, the median lethal dose of the tested compound was expected to be greater than 500 mg/kg. No significant change occurred in selected biomarkers, except BUN and RBS levels, but a histopathological study showed moderate toxic effect on liver, kidney, pancreas and heart tissues by the cyclohexenone derivative.
Noble Metal Organometallic Complexes Display Antiviral Activity against SARS-CoV-2
Authors: Chuong Christina , DuChane Christine M. , Webb Emily M. , Rai Pallavi , Marano Jeffrey M. , Bernier Chad M. , Merola Joseph S. , Weger-Lucarelli James
Published: May 2021

SARS-CoV-2 emerged in 2019 as a devastating viral pathogen with no available preventative or treatment to control what led to the current global pandemic. The continued spread of the virus and increasing death toll necessitate the development of effective antiviral treatments to combat this virus. To this end, we evaluated a new class of organometallic complexes as potential antivirals. Our findings demonstrate that two pentamethylcyclopentadienyl (Cp*) rhodium piano stool complexes, Cp*Rh(1,3-dicyclohexylimidazol-2-ylidene)Cl2 (complex 2) and Cp*Rh(dipivaloylmethanato)Cl (complex 4), have direct virucidal activity against SARS-CoV-2. Subsequent in vitro testing suggests that complex 4 is the more stable and effective complex and demonstrates that both 2 and 4 have low toxicity in Vero E6 and Calu-3 cells. The results presented here highlight the potential application of organometallic complexes as antivirals and support further investigation into their activity.
Multiple Mammarenaviruses Circulating in Angolan Rodents
Authors: Těšíková Jana , Krásová Jarmila , Goüy de Bellocq Joëlle
Published: May 2021

Rodents are a speciose group of mammals with strong zoonotic potential. Some parts of Africa are still underexplored for the occurrence of rodent-borne pathogens, despite this high potential. Angola is at the convergence of three major biogeographical regions of sub-Saharan Africa, each harbouring a specific rodent community. This rodent-rich area is, therefore, strategic for studying the diversity and evolution of rodent-borne viruses. In this study we examined 290 small mammals, almost all rodents, for the presence of mammarenavirus and hantavirus RNA. While no hantavirus was detected, we found three rodent species positive for distinct mammarenaviruses with a particularly high prevalence in Namaqua rock rats (Micaelamys namaquensis). We characterised four complete virus genomes, which showed typical mammarenavirus organisation. Phylogenetic and genetic distance analyses revealed: (i) the presence of a significantly divergent strain of Luna virus in Angolan representatives of the ubiquitous Natal multimammate mouse (Mastomys natalensis), (ii) a novel Okahandja-related virus associated with the Angolan lineage of Micaelamys namaquensis for which we propose the name Bitu virus (BITV) and (iii) the occurrence of a novel Mobala-like mammarenavirus in the grey-bellied pygmy mouse (Mus triton) for which we propose the name Kwanza virus (KWAV). This high virus diversity in a limited host sample size and in a relatively small geographical area supports the idea that Angola is a hotspot for mammarenavirus diversity.
Influence of Selective Agents (EMJH-STAFF), Sample Filtration and pH on Leptospira interrogans Serovar Icterohaemorrhagiae Cultivation and Isolation from Swine Urine
Authors: Steinparzer Romana , Mair Tamara , Unterweger Christine , Steinrigl Adi , Schmoll Friedrich
Published: May 2021

Leptospira spp. cause the zoonotic disease leptospirosis, which occurs in numerous mammalians worldwide. Isolation is still important for serotyping and genotyping of Leptospira, which in turn is essential for epidemiological surveillance of leptospirosis and the development of diagnostic tests and vaccines. However, isolation of Leptospira from clinical specimens is inherently insensitive. This study was conducted to examine the influence of selective agents, sample filtration, sample pH and the use of phosphate buffered saline (PBS) buffer for sample storage to improve the success of cultivation and isolation of Leptospira interrogans serovar Icterohaemorrhagiae from swine urine. EMJH (Ellinghausen McCullough, Johnson and Harris) medium including the selective agents sulfamethoxazole, trimethoprim, amphotericin, fosfomycin and 5-fluorouracil (STAFF) increased the success of Leptospira isolation from spiked swine urine samples. Sample filtration yielded only negative results. Isolation in EMJH-STAFF was successful from swine urine with a density as low as 104 Leptospira/mL, and urine with pH ≤ 7 impaired the cultivation rate. Cultivation and isolation were not improved by the addition of PBS to spiked urine samples prior to storage for 24 h at 4 °C. The results of the study demonstrate that cultivation and isolation of leptospires from swine urine can be improved by enhanced methods.
Measuring contamination levels in external induced atmosphere of Russian orbital stations and the Russian Segment of the International Space Station
Authors: DUSHIN Vladimir K. , KRYLOV Andrey N. , SKOROVAROV Aleksandr Yu.
Published: May 2021

In view of the increasing number of science experiments being carried out in the Russian Segment of the International Space Station, there is a growing interest in the state of the environment where they are conducted, especially, in the contaminating effects of the external induced atmosphere. Sources of the induced atmosphere contamination are the off-gassing of non-metallic materials of the outer coatings, firings of control system thrusters and exhausts of various venting systems, as well as dust particles remaining on the external surfaces after launch from Earth, atmospheric aerosols and space dust. The paper presents results of measurements of molecular depositions on the sensors of quartz crystal microbalances that were used for evaluating contamination in the induced atmosphere of the first Soviet orbital stations Salyut-7 and Mir, as well as modules Pirs and Poisk of the Russian Segment of the International Space Station. The results of the experiments show a significant effect of lighting and thermal conditions of the contamination source and the quartz microbalance sensor on the measurement results.
Key words: contamination, quartz crystal microbalance, orbital, external induced atmosphere.
The small spacecraft with a magnetic sail on high-temperature superconductors
Authors: KOMBAEV Timur Sh. , ARTEMOV Mikhail K. , SYSOEV Valentin K. , DEZHIN Dmitry S.
Published: May 2021

It is proposed to develop a small spacecraft for an experiment using high-temperature superconductors (HTS) and shape memory materials. The purpose of the experiment is to test a technological capability of creating a strong magnetic field on the small spacecraft using HTS and shape memory materials for deployed large-area structures, and study the magnetic field interaction with the solar wind plasma and the resulting force impact on the small spacecraft. This article is of a polemical character and makes it possible to take a fresh look at the applicability of new technologies in space-system engineering.
Key words: high-temperature superconductors, shape memory materials, solar wind, spacecraft.