bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of the COVID-19 Pandemic on the Detection of Leprosy in Micro-Regions with a High Risk of Illness in Minas Gerais, Brazil
Authors: Vidal Sarah Lamas , Lages Daniele dos Santos , Maciel Isabela Cristina Lana , Leite Isabel Cristina Gonçalves , Coelho Angélica da Conceição Oliveira , Lana Francisco Carlos Félix
Published: November 2024

Background: Leprosy control remains challenging in Brazil and has been aggravated by the COVID-19 pandemic. Objective: To analyze the impact of the COVID-19 pandemic on the epidemiological scenario of leprosy through the detection rate of new cases, the risk of illness, and the hidden prevalence of leprosy according to high-risk micro-region in Minas Gerais, Brazil. Methods: An ecological study conducted in the health micro-regions of Minas Gerais, using data on new leprosy cases diagnosed between 2015 and 2023. The annual detection rate of new cases, the risk of illness index and the hidden prevalence of leprosy were evaluated. The time trend was evaluated by calculating the annual percentage change (APC) of the detection rate, based on segmented linear regression, considered significant when it showed p < 0.05. Results: The state of Minas Gerais showed a significant negative annual increase between 2015 and 2020 (APC = −7.91; 95%CI −21.76–−1.72), and in 2020 it showed an inflection point, with an annual increase of 9.91 in the period from 2020 to 2023. When evaluating the hidden prevalence of leprosy in Minas Gerais, we observed a reduction in the estimates’ average from 2015–2019 (2.78) to 2020–2023 (2.00). The state as a whole showed an upward trend in the risk of illness, with the average index varying from 0.28 (medium risk) to 0.55 (high risk). Conclusions: The pandemic has had a considerable and heterogeneous impact on the detection of new cases, as well as on the risk of becoming ill and the hidden prevalence of leprosy, with repercussions for the control of the endemic in populations. There is a need to implement public health policies that prioritize the early identification of cases and ensure that vulnerable populations are monitored.
Study Abroad Angst: A Literature Review on the Mental Health of International Students During COVID-19
Authors: Akiba Daisuke , Perrone Michael , Almendral Caterina
Published: November 2024

Background: The COVID-19 pandemic presented unique and unprecedented challenges for international students, those studying at institutions of higher education outside of their home countries, due to their distinct circumstances and vulnerabilities. This literature review examines the multifaceted mental health burdens they experienced and highlights the need for targeted support and interventions. Methods: A rigorous search across three databases (i.e., PubMed, PsycINFO, and ERIC) yielded 50 empirical studies for inclusion in this literature review. A six-phase thematic analysis framework was employed to identify and synthesize key themes. Results: Seven prominent themes emerged: (1) academic and professional disruptions; (2) challenges navigating international student status; (3) social isolation and loneliness; (4) difficulties with living arrangements; (5) financial and food insecurity; (6) health concerns for self and loved ones; and (7) experiences of discrimination and xenophobia. Conclusions: This review highlights a range of tolls that mental health consequences took on international students, and it suggests the need for targeted interventions and support services to address these challenges. It also identifies critical research gaps, such as the need for longitudinal studies and comparative analyses with domestic students. The implications for inclusive policies and supportive environments to promote international students’ well-being are discussed.
Increased Risk of Dentoalveolar Trauma in Patients with Autism Spectrum Disorder: A Systematic Review with Meta-Analysis
Authors: da Costa Izabela , Junqueira Rafael Binato , Faé Daniele Sorgatto , de Souza Luisa Amorim Pêgas , Lemos Cleidiel Aparecido Araujo
Published: November 2024

The prevalence of traumatic dental injuries (TDI) in patients with autism spectrum disorders (ASD) remains unclear. Given these discrepancies, an updated review of the evidence on the risk of TDI in patients with ASD is essential. This systematic review and meta-analysis aimed to evaluate the prevalence of TDI in patients with ASD and compare it to that in neurotypical patients. This study protocol was registered on PROSPERO (CRD42024580127) and followed the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA guidelines. A comprehensive search of four databases—MEDLINE/PubMed, Web of Science, Scopus, and Embase—was conducted for articles published up to August 2024. Moreover, the gray literature (ProQuest) and reference lists were screened. The inclusion criteria required participants with ASD to assess TDI across deciduous, mixed, and permanent dentition regardless of age. No restrictions were applied on TDI type, language, or publication date. Additionally, case reports, reviews, letters, and studies addressing other oral disorders without specific TDI data were excluded. A single-arm meta-analysis evaluated the cumulative proportion and 95% confidence interval (CI) of TDI in patients with ASD. Moreover, a comparative meta-analysis was performed to assess the risk of TDI between ASD and neurotypical patients, calculating the odds ratio (OR) with a 95% CI, and a p < 0.05 was deemed significant, using the R program. Quality assessment was performed using the National Heart, Lung, and Blood Institute tool, and the certainty of evidence was evaluated using GRADE. A total of 22 studies were included to determine the overall prevalence of TDI, of which 16 studies directly compared patients with ASD to neurotypical individuals. In total, 3817 participants were evaluated, including 2162 individuals with ASD and 1655 neurotypical patients. A single-arm meta-analysis estimated a TDI prevalence of 22% (Confidence Interval [CI]: 17–27%) among patients with ASD. A significant difference in the risk was observed between ASD and neurotypical patients (p = 0.003; Odds Ratio [OR]: 1.67; CI: 1.19–2.26). However, substantial heterogeneity was observed in this analysis. Although the majority of studies were rated as high quality, the certainty of the evidence was considered very low. Despite the limitations of this study, the findings suggest that patients with ASD are at a higher risk of developing TDI than the risk observed in neurotypical patients. Therefore, preventive educational initiatives are recommended to reduce the risk of TDI in this population.
Multi-Professional Family Support Programme: A Collective Development at the Neonatal Intensive Care Unit
Authors: Gomes de Souza Bruna , Nascimento Luciana , Fioresi Mirian , Furieri Lorena , Balbino Flávia , Andrade Luísa , Bringuente Maria
Published: November 2024

This study was undertaken to structure and validate a Multi-Professional Family Support Programme that was collectively developed at a Neonatal Intensive Care Unit (NICU). This is participative research of the action-research category with a qualitative–quantitative approach conducted at a University Hospital in the southeast of Brazil with the participation of their multi-professional staff. The study was done in four interdependent stages, adapted from the method proposed by Thiollent (2011): organisation, structuring, validation, and diffusion. Qualitative data were analysed following Bardin’s (2016) Content Analysis Technique and presented in categories and sub-categories. The evaluation instruments followed the evaluation criteria proposed by Pasquali (2010). In the analysis of quantitative data, Cronbach’s Alpha coefficient was used to verify reliability, Fleiss’ Kappa coefficient for measuring agreement, and the Content Validity Index for relevance and representativeness. The programme was built collectively and is based on the Family-Centred Care model. Cronbach’s Alpha reached values above 0.90, which is excellent reliability. There was varying agreement between substantial/perfect and significant (k = 0.68–1.00; p < 0.001), and all the evaluation criteria were considered relevant and representative (CVI > 90.0%). The programme and its guiding technologies were structured and validated with high levels of reliability, agreement, relevance, and representativeness.
Identification and Characterization of a Novel CCDC6::CASP7 Gene Rearrangement in an Advanced Colorectal Cancer Patient: A Case Report
Authors: Montero Juan Carlos , Tur Raquel , Jiménez-Perez Andrea , Filipovich Elena , Alcaraz Susana , Rodríguez Marta , Abad Mar , Sayagués José María
Published: November 2024

Despite the existence of effective therapy options for patients with localized colorectal cancer, advanced-stage patients have limited therapies. Genomic profiling is a promising tool for guiding treatment selection as well as patient monitoring. Here, we describe a novel gene rearrangement (CCDC6::CASP7) detected in a patient with advanced colorectal cancer that could be a therapeutic target. The patient underwent surgical resection but died after the operation from fecal peritonitis. To our knowledge, this is the first report in which the CCDC6::CASP7 gene rearrangement has been described in an advanced colorectal adenocarcinoma patient.
NS2B-D55E and NS2B-E65D Variations Are Responsible for Differences in NS2B-NS3 Protease Activities Between Japanese Encephalitis Virus Genotype I and III in Fluorogenic Peptide Model
Authors: Wahaab Abdul , Zhang Yan , Liu Ke , Rasgon Jason L. , Kang Lei , Hameed Muddassar , Li Chenxi , Anwar Muhammad Naveed , Zhang Yanbing , Shoaib Anam , Li Beibei , Qiu Yafeng , Wei Jianchao , Ma Zhiyong
Published: November 2024

Japanese encephalitis virus (JEV) NS2B-NS3 is a protein complex composed of NS3 proteases and an NS2B co-factor. The N-terminal protease domain (180 residues) of NS3 (NS3(pro)) interacts directly with a central 40-amino acid hydrophilic domain of NS2B (NS2B(H)) to form an active serine protease. In this study, the recombinant NS2B(H)-NS3(pro) proteases were prepared in E. coli and used to compare the enzymatic activity between genotype I (GI) and III (GIII) NS2B-NS3 proteases. The GI NS2B(H)-NS3(pro) was able to cleave the sites at the internal C, NS2A/NS2B, NS2B/NS3, and NS3/NS4A junctions that were identical to the sites proteolytically processed by GIII NS2B(H)-NS3(pro). Analysis of the enzymatic activity of recombinant NS2B(H)-NS3(pro) proteases using a model of fluorogenic peptide substrate revealed that the proteolytical processing activity of GIII NS2B(H)-NS3(pro) was significantly higher than that of GI NS2B(H)-NS3(pro). There were eight amino acid variations between GI and GIII NS2B(H)-NS3(pro), which may be responsible for the difference in enzymatic activities between GI and GIII proteases. Therefore, recombinant mutants were generated by exchanging the NS2B(H) and NS3(pro) domains between GI and GIII NS2B(H)-NS3(pro) and subjected to protease activity analysis. Substitution of NS2B(H) significantly altered the protease activities, as compared to the parental NS2B(H)-NS3(pro), suggesting that NS2B(H) played an essential role in the regulation of NS3(pro) protease activity. To further identify the amino acids responsible for the difference in protease activities, multiple substitution mutants including the individual and combined mutations at the variant residues 55 and 65 of NS2B(H) were generated and subjected to protease activity analysis. Replacement of NS2B-55 and NS2B-65 of GI to GIII significantly increased the enzymatic activity of GI NS2B(H)-NS3(pro) protease, whereas mutation of NS2B-55 and NS2B-65 of GIII to GI remarkably reduced the enzymatic activity of GIII NS2B(H)-NS3(pro) protease. Overall, these data demonstrated that NS2B-55 and NS2B-65 variations in the hydrophilic domain of NS2B co-contributed to the difference in NS2B(H)-NS3(pro) protease activities between GI and GIII. However, it will be crucial to explore these mutations in other in vivo and/or in vitro models. Collectively, these observations will be useful for understanding the replication of JEV GI and GIII viruses.
Root Microbiome and Metabolome Traits Associated with Improved Post-Harvest Root Storage for Sugar Beet Breeding Lines Under Southern Idaho Conditions
Authors: Majumdar Rajtilak , Kandel Shyam L. , Strausbaugh Carl A. , Singh Anuradha , Pokhrel Suresh , Bill Malick
Published: November 2024

Post-harvest storage loss in sugar beets due to root rot and respiration can cause >20% sugar loss. Breeding strategies focused on factors contributing to improved post-harvest storage quality are of great importance to prevent losses. Using 16S rRNA and ITS sequencing and sugar beet mutational breeding lines with high disease resistance (R), along with a susceptible (S) commercial cultivar, the role of root microbiome and metabolome in storage performance was investigated. The R lines in general showed higher abundances of bacterial phyla, Patescibacteria at the M time point, and Cyanobacteria and Desulfobacterota at the L time point. Amongst fungal phyla, Basidiomycota (including Athelia) and Ascomycota were predominant in diseased samples. Linear discriminant analysis Effect Size (LEfSe) identified bacterial taxa such as Micrococcales, Micrococcaceae, Bacilli, Glutamicibacter, Nesterenkonia, and Paenarthrobacter as putative biomarkers associated with resistance in the R lines. Further functional enrichment analysis showed a higher abundance of bacteria, such as those related to the super pathway of pyrimidine deoxyribonucleoside degradation, L-tryptophan biosynthesis at M and L, and fungi, such as those associated with the biosynthesis of L-iditol 2-dehydrogenase at L in the R lines. Metabolome analysis of the roots revealed higher enrichment of pathways associated with arginine, proline, alanine, aspartate, and glutamate metabolism at M, in addition to beta-alanine and butanoate metabolism at L in the R lines. Correlation analysis between the microbiome and metabolites indicated that the root’s biochemical composition, such as the presence of nitrogen-containing secondary metabolites, may regulate relative abundances of key microbial candidates contributing to better post-harvest storage.
Transcriptomic Response of the Ovarian Follicle Complex in Post-Vitellogenic Rainbow Trout to 17α,20β-Dihdroxy-4-pregnen-3-one In Vitro
Authors: Ma Hao , Gao Guangtu , Palti Yniv , Tripathi Vibha , Birkett Jill E. , Weber Gregory M.
Published: November 2024

Gonadotropins and progestins are the primary regulators of follicle maturation and ovulation in fish, and they require complex communication among the oocyte and somatic cells of the follicle. The major progestin and the maturation-inducing hormone in salmonids is 17α,20β-dihdroxy-4-pregnen-3-one (17,20βP), and traditional nuclear receptors and membrane steroid receptors for the progestin have been identified within the follicle. Herein, RNA-seq was used to conduct a comprehensive survey of changes in gene expression throughout the intact follicle in response to in vitro treatment with these hormones to provide a foundation for understanding the coordination of their actions in regulating follicle maturation and preparation for ovulation. A total of 5292 differentially expressed genes were identified from our transcriptome sequencing datasets comparing four treatments: fresh tissue; untreated control; 17,20βP-treated; and salmon pituitary homogenate-treated follicles. Extensive overlap in affected genes suggests many gonadotropin actions leading to the acquisition of maturational and ovulatory competence are mediated in part by gonadotropin induction of 17,20βP synthesis. KEGG analysis identified signaling pathways, including MAPK, TGFβ, FoxO, and Wnt signaling pathways, among the most significantly enriched pathways altered by 17,20βP treatment, suggesting pervasive influences of 17,20βP on actions of other endocrine and paracrine factors in the follicle complex.
Binding of MAP3773c Protein of Mycobacterium avium subsp. paratuberculosis in the Mouse Ferroportin1 Coding Region
Authors: Dueñas Mena Dulce Liliana , Gutiérrez-Pabello José A. , Quintero Chávez Kaina , Brito-Perea Mirna Del Carmen , Díaz Padilla Dania Melissa , Cortez Hernández Omar , Chávez Mendez José Román , Alcalá Zacarias Jocelyn Marcela , Vela Sancho Giselle Berenice , Landeros Sánchez Bertha
Published: November 2024

Mycobacterium avium subsp. paratuberculosis (MAP) is known to cause paratuberculosis. One notable protein, MAP3773c, plays a critical role in iron metabolism as a transcription factor. This study aims to investigate the binding affinity of MAP3773c to the chromatin of the Ferroportin1 (FPN1) gene in murine macrophage J774 A.1. We conducted a sequence alignment to identify potential interaction sites for MAP3773c. Following this, we used in silico analysis to predict binding interactions, complemented by electrophoretic mobility shift assay (EMSA) to confirm in vitro binding of MAP3773c. The map3773c gene was cloned into the pcDNA3.1 vector, with subsequent expression analysis carried out via Western blotting and real-time PCR. Chromatin immunoprecipitation (CHiP) assays were performed on transfected macrophages to confirm binding in the native chromatin context. Our in silico and in vitro analysis indicated that MAP3773c interacts with two binding motifs within the FPN1 coding region. The ChiP results provided additional validation, demonstrating the binding of MAP3773c to the FPN1 chromatin through successful amplification of the associated chromatin fragment via PCR. Our study demonstrated that MAP3773c binds to FPN1 and provides insight into the role of MAP3773c and its effect on host iron transport.
Correction: Liu et al. Optimization of Antibacterial Activity and Biosafety through Ultrashort Peptide/Cyclodextrin Inclusion Complexes. Int. J. Mol. Sci. 2023, 24, 14801
Authors: Liu Hang , Wang Lin , Yao Chen
Published: November 2024

In the original publication [...]
Cytokinesis-Blocking Micronucleus Assay for Assessing Nuclear Chromatin Integrity Abnormalities in Dog’s Somatic Cells After Exposure to HVAD-Produced Silver Nanoparticles
Authors: Grzesiakowska-Dul Anna , Kasprowicz Marek Jan , Otwinowska-Mindur Agnieszka , Baran Przemysław , Kuchta-Gładysz Marta
Published: November 2024

The widespread use of silver nanoparticles in many industries is increasing every year. Along with this use, there is growing concern about the potential unintentional exposure of human and animal organisms to these nanomaterials. It has been shown that AgNPs have the ability to penetrate organisms and can have harmful effects on cells and organs in the body. In order to reduce the effects of AgNPs on living organisms, newer solutions are being investigated, such as particle stabilization or other methods of synthesizing these particles. The physical synthesis of AgNPs using high-voltage arc discharge (HVAD) may be one of these alternatives. To determine the effect of silver nanoparticles obtained by this method, cytogenetic analysis was performed on domestic dog somatic cells using a cytokinesis-blocking micronucleus assay. In the experiments performed, peripheral blood cells of the domestic dog were exposed in vitro for 3 and 24 h to three tested colloidal silver compounds (unstable AgNP-HVAD, sodium citrate-stabilized silver nanoparticles—AgNP+C, and silver nitrate). The toxicity of these compounds was evaluated at concentrations of 5, 10, and 20 µg/L, and the presence of the following cellular abnormalities was analyzed: micronuclei, nuclear buds, nucleoplasmic bridges, or multinucleated cells. The study showed a significant increase in the number of micronuclei compared to the control sample, as well as the presence of nuclear buds and nucleoplasmic bridges in somatic cells of the domestic dog, confirming the genotoxic nature of the particles. However, there was no cytotoxic effect due to the lower number of multinucleated cells and the absence of apoptotic or necrotic cells in the samples analyzed. Further studies are needed to better understand the mechanisms of toxicity of AgNPs produced by the HVAD method and the extent of their effects on mammalian somatic cells.
Sustainable Solid-State Sodium-Ion Batteries Featuring Ferroelectric Electrolytes
Authors: Freitas Ângela , Baptista Manuela C. , Braga Maria Helena
Published: November 2024

Solid-state batteries offer significant advantages but present several challenges. Given the complexity of these systems, it is good practice to begin the study with simpler models and progressively advance to more complex configurations, all while maintaining an understanding of the physical principles governing solid-state battery operation. The results presented in this work pertain to cells without traditional electrodes, thus providing a foundation for guiding the development of fully functional solid-state cells. The open circuit voltage (OCV) of the Cu/Na2.99Ba0.005ClO composite in a cellulose/Zn pouch cell achieves 1.10 V, reflecting the difference in the chemical potentials of the current collectors (CCs), Zn and Cu, serving as electrodes. After 120 days, while set to discharge, conversely to what was expected, a higher potential difference of 1.13 V was attained (capacity of 5.9 mAh·g−1electrolyte). By incorporating a layer of carbon felt, the OCV became 0.85 V; however, after 95 days, the potential difference increased to 1.20 V. Ab initio simulations were additionally performed on a Cu/Na3ClO/Zn heterojunction showing the formation of dipoles and the Na deposition on Zn which is demonstrated experimentally. The sodium plating on the negative CC (Zn) takes place as the cell is set to discharge at room temperature but is not observed at 40 °C.
The RNA Revolution in the Central Molecular Biology Dogma Evolution
Authors: Haseltine William A. , Patarca Roberto
Published: November 2024

Human genome projects in the 1990s identified about 20,000 protein-coding sequences. We are now in the RNA revolution, propelled by the realization that genes determine phenotype beyond the foundational central molecular biology dogma, stating that inherited linear pieces of DNA are transcribed to RNAs and translated into proteins. Crucially, over 95% of the genome, initially considered junk DNA between protein-coding genes, encodes essential, functionally diverse non-protein-coding RNAs, raising the gene count by at least one order of magnitude. Most inherited phenotype-determining changes in DNA are in regulatory areas that control RNA and regulatory sequences. RNAs can directly or indirectly determine phenotypes by regulating protein and RNA function, transferring information within and between organisms, and generating DNA. RNAs also exhibit high structural, functional, and biomolecular interaction plasticity and are modified via editing, methylation, glycosylation, and other mechanisms, which bestow them with diverse intra- and extracellular functions without altering the underlying DNA. RNA is, therefore, currently considered the primary determinant of cellular to populational functional diversity, disease-linked and biomolecular structural variations, and cell function regulation. As demonstrated by RNA-based coronavirus vaccines’ success, RNA technology is transforming medicine, agriculture, and industry, as did the advent of recombinant DNA technology in the 1980s.
USP8 Mutations Associated with Cushing’s Disease Alter Protein Structure Dynamics
Authors: Petukhova Natalia , Poluzerova Anastasia , Bug Dmitry , Nerubenko Elena , Kostareva Anna , Tsoy Uliana , Dmitrieva Renata
Published: November 2024

The adenomas in Cushing’s disease frequently exhibit mutations in exon 14, within a binding motif for the regulatory protein 14-3-3 located between the catalytic domain (DUB), responsible for ubiquitin hydrolysis, and the WW-like domain that mediates autoinhibition, resulting in constantly active USP8. The exact molecular mechanism of deubiquitinase activity disruption in Cushing’s disease remains unclear. To address this, Sanger sequencing of USP8 was performed to identify mutations in corticotropinomas. These mutations were subjected to computational screening, followed by molecular dynamics simulations to assess the structural alterations that might change the biological activity of USP8. Eight different variants of the USP8 gene were identified both within and outside the “hotspot” region. Six of these had previously been reported in Cushing’s disease, while two were detected for the first time in our patients with CD. One of the two new variants, initially classified as benign during screening, was found in the neighboring SH3 binding motif at a distance of 20 amino acids. This variant demonstrated pathogenicity patterns similar to those of known pathogenic variants. All USP8 variants identified in our patients caused conformational changes in the USP8 protein in a similar manner. The identified mutations, despite differences in annotation results—including evolutionary conservation assessments, automated predictor data, and variations in localization within exon 14—exhibit similar patterns of protein conformational change. This suggests a pathogenic effect that contributes to the development of CD.
Mechanisms of Adsorption of Phenoxyalkanoic Herbicides on Fulvic and Humic Acids
Authors: Paszko Tadeusz , Matysiak Joanna , Spadotto Claudio A. , Boguta Patrycja , Skic Kamil
Published: November 2024

Our recent study demonstrated that fulvic and humic acids are the major contributors to the adsorption of phenoxyalkanoic acid herbicides in soils. At very low pH, the neutral forms of these herbicides are bound directly to fulvic and humic acids, whereas at higher pH, their anionic forms are adsorbed mainly via bridges created by Al3+ species. The number of active sorption sites associated with Al3+ species complexed with fulvic acids is pH-dependent, whereas the number of corresponding sites in humic acids is pH-independent. Based on the results of the FTIR analysis, research into adsorption thermodynamics, and molecular modeling, an attempt was made in the present study to explain the adsorption mechanisms of six phenoxyalkanoic herbicides used currently in the European Union on the surfaces of the above fractions of humic substances. The obtained values of standard enthalpy (ΔH0) for the adsorption of the anionic forms of phenoxyalkanoic herbicides on fulvic or humic acids complexed with Al3+ were in the range of physical adsorption, i.e., from −8.4 kJ/mol to −2.9 kJ/mol for the former, and from −5.3 kJ/mol to −2.4 kJ/mol for the latter. The study demonstrated that the neutral forms of phenoxyalkanoic herbicides were bound to humic substances mainly via H-bonds, π-π stacking interactions, and hydrophobic interactions. Al3+ species were complexed with fulvic and humic acids to form outer-sphere complexes. Ternary outer-sphere complexes were also created between the anionic forms of phenoxyalkanoic acid herbicides and positively charged Al3+ species complexed with fulvic acids. The mechanisms of adsorption on humic acids involved a ligand exchange between a loosely bound hydroxyl group of hydrolyzed Al3+ complexed with this adsorbent and the anionic form of the herbicide. However, in this case, adsorption took place only in the presence of sufficiently strong hydrophobic and π-π stacking interactions supported by H-bonds. These findings elucidate why phenoxyalkanoic herbicides are mobile in the soil profile and are often rapidly degraded in soils.
Molecular Characterization of Subdomain Specification of Cochlear Duct Based on Foxg1 and Gata3
Authors: Gil Yongjin , Ryu Jiho , Yang Hayoung , Ma Yechan , Nam Ki-Hoan , Jang Sung-Wuk , Shim Sungbo
Published: November 2024

The inner ear is one of the sensory organs of vertebrates and is largely composed of the vestibule, which controls balance, and the cochlea, which is responsible for hearing. In particular, a problem in cochlear development can lead to hearing loss. Although numerous studies have been conducted on genes involved in the development of the cochlea, many areas still need to be discovered regarding factors that control the patterning of the early cochlear duct. Herein, based on the dynamic expression pattern of FOXG1 in the apical and basal regions of the E13.5 cochlear duct, we identified detailed expression regions through an open-source analysis of single-cell RNA analysis data and demonstrated a clinical correlation with hearing loss. The distinct expression patterns of FOXG1 and GATA3 during the patterning process of the cochlear duct provide important clues to understanding how the fates of the apical and basal regions are divided. These results are expected to be extremely important not only for understanding the molecular mechanisms involved in the early development of the cochlear duct, but also for identifying potential genes that cause hearing loss.
Design, Synthesis and Biological Exploration of Novel N-(9-Ethyl-9H-Carbazol-3-yl)Acetamide-Linked Benzofuran-1,2,4-Triazoles as Anti-SARS-CoV-2 Agents: Combined Wet/Dry Approach Targeting Main Protease (Mpro), Spike Glycoprotein and RdRp
Authors: Zahoor Ameer Fawad , Munawar Saba , Ahmad Sajjad , Iram Fozia , Anjum Muhammad Naveed , Khan Samreen Gul , Javid Jamila , Nazeer Usman , Bhat Mashooq Ahmad
Published: November 2024

A novel series of substituted benzofuran-tethered triazolylcarbazoles was synthesized in good to high yields (65–89%) via S-alkylation of benzofuran-based triazoles with 2-bromo-N-(9-ethyl-9H-carbazol-3-yl)acetamide. The inhibitory potency of the synthesized compounds against SARS-CoV-2 was evaluated by enacting molecular docking against its three pivotal proteins, namely, Mpro (main protease; PDB ID: 6LU7), the spike glycoprotein (PDB ID: 6WPT), and RdRp (RNA-dependent RNA polymerase; PDB ID: 6M71). The docking results indicated strong binding affinities between SARS-CoV-2 proteins and the synthesized compounds, which were thereby expected to obstruct the function of SARS proteins. Among the synthesized derivatives, the compounds 9e, 9h, 9i, and 9j exposited the best binding scores of −8.77, −8.76, −8.87, and −8.85 Kcal/mol against Mpro, respectively, −6.69, −6.54, −6.44, and −6.56 Kcal/mol against the spike glycoprotein, respectively, and −7.61, −8.10, −8.01, and −7.54 Kcal/mol against RdRp, respectively. Furthermore, the binding scores of 9b (−8.83 Kcal/mol) and 9c (−8.92 Kcal/mol) against 6LU7 are worth mentioning. Regarding the spike glycoprotein, 9b, 9d, and 9f expressed high binding energies of −6.43, −6.38, and −6.41 Kcal/mol, accordingly. Correspondingly, the binding affinity of 9g (−7.62 Kcal/mol) against RdRp is also noteworthy. Furthermore, the potent compounds were also subjected to ADMET analysis to evaluate their pharmacokinetic properties, suggesting that the compounds 9e, 9h, 9i, and 9j exhibited comparable values. These potent compounds may be selected as inhibitory agents and provide a pertinent context for further investigations.
Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology
Authors: Huang Yuebing , Qiu Hai , Chen Qianling , Meng Zilin , Qiao Dongfang , Yue Xia
Published: November 2024

Mechanical asphyxia presents a challenging diagnostic issue in forensic medicine due to its often covert nature, and the signs visible during an autopsy are usually not specific. Despite some progress in understanding hypoxia’s effects, traditional methods’ inherent limitations might overlook new biomarkers in mechanical asphyxia. This study employed 4D-DIA proteomics to explore the protein expression profiles of cardiac samples under conditions of mechanical asphyxia. Proteomic analysis identified 271 and 371 differentially expressed proteins in the strangulation and suffocation groups, respectively, compared to the control group. Seventy-eight differentially expressed proteins were identified across different mechanical asphyxia groups compared to the control group. GO and KEGG analysis showed enrichment in pathways, including complement and coagulation cascades, cAMP and cGMP-PKG signaling pathways, inflammatory mediator regulation of TRP channels, and phagosomes. Through stringent selection based on protein interactions, ALKBH5, NAA10, and CLPB were identified as potential diagnostic biomarkers. ALKBH5 showed increased expression in asphyxia models, while NAA10 and CLPB were downregulated; these biomarker changes were validated in both animal models and human cardiac samples. This study highlights the potential of proteomics in discovering reliable biomarkers, which can enhance the specificity of mechanical asphyxia diagnosis in forensic practice, provide new insights into the pathophysiological mechanisms of mechanical asphyxia, and offer new perspectives for diagnosing mechanical asphyxia.
A New Approach for Orbital Wall Reconstruction in a Rabbit Animal Model Using a Hybrid Hydroxyapatite–Collagen-Based Implant
Authors: Vasile Victor A. , Istrate Sinziana , Cursaru Laura-Madalina , Piticescu Roxana M. , Ghita Aurelian M. , Popescu Diana M. , Garhöfer Gerhard , Catrina Ana M. , Spandole-Dinu Sonia , Haidoiu Cerasela , Suhaianu Vladimir , Voinea Oana C. , Dragut Dumitru Valentin , Popa-Cherecheanu Alina
Published: November 2024

Reconstructing the orbit following complex craniofacial fractures presents significant challenges. Throughout the years, several materials have been used for orbital reconstruction, taking into account factors such as their durability, compatibility with living tissue, cost efficiency, safety, and capacity to be adjusted during surgery. Nevertheless, a consensus has not yet been reached on the optimal material for orbital restoration. This study investigates the potential of a hybrid hydroxyapatite–collagen (HAp-COL) material 3D-printed on Ti mesh to be used as an implant for orbital wall reconstruction. HAp-COL powder was synthesized using a high-pressure hydrothermal technique. The powder was further used to 3D-print HAp-COL structures on titanium mesh, with the latter having potential uses in orbital wall reconstruction. Biocompatibility was assessed by evaluating the effects of the HAp-COL material on the adhesion and proliferation of fibroblasts (3T3) and mesenchymal stem cells (MSCs) in culture. In vitro and in vivo results showed that HAp-COL is highly biocompatible and has a good integration of the implant in the bone. The findings reported in this study offer convincing evidence to support the use of our designed HAp-COL for the restoration of orbital wall fractures, with a high level of safety.
Peculiarities of Plant Mineral Composition in Semi-Desert Conditions
Authors: Golubkina Nadezhda , Sheshnitsan Sergey , Koshevarov Andrew , Pirogov Nikolay , Plotnikova Ulyana , Tallarita Alessio Vincenzo , Murariu Otilia Cristina , Merlino Luca , Caruso Gianluca
Published: November 2024

Plant–soil interactions in semi-desert conditions elicit the development of plant-specific adaptation strategies, including selective accumulation of macro- and microelements. Using an ICP-MS analysis of 12 plant species belonging to Asteraceae, Fabaceae, Poaceae, Ephedraceae, Amarantaceae, and Lamiaceae families of the Baskunchak Nature Reserve, remarkable species differences in accumulation of 22 macro- and microelements were recorded. The most common Artemisia species and Poaceae representatives belong to two different groups of plants with high content of Na, K, Zn, Cu, V and high antioxidant status and low Si typical for the former group and the opposite characteristics for the latter one. The mentioned phenomenon indicates two diverse powerful adaptation mechanisms based on the antioxidant defense and Si protection, respectively. The high frequency of remarkable levels of Se in plants with BCF exceeding 1 (Glycyrrhiza aspera, Phlomis pungens, Tanacetum nullifolium, Helichrysum nogaicum, and Jurinea ewersmannii), Zn in all species except Poa angustifolia, and Cu in the Asteraceae plants Phlomis pungens and Krascheninnikovia ceratoides suggests the significance of these elements in plant tolerance to environmental stresses. Plant–soil positive correlations were recorded for Sr (r = 0.866; p < 0.001); plant Sr, Fe, Co, Pb levels and soil salinity (r = 0.763, p < 0.001; r = 0.606, p < 0.03; r = 0.627, p < 0.02; r = 0.548, p < 0.05, respectively); and Cr only for Asteraceae species (r = 0.986, p < 0.001). The results obtained in this research may be used in plant adaptability evaluation in conditions of environmental stress.
Exploring the Impact of Image-Based Audio Representations in Classification Tasks Using Vision Transformers and Explainable AI Techniques
Authors: Masri Sari , Hasasneh Ahmad , Tami Mohammad , Tadj Chakib
Published: November 2024

An important hurdle in medical diagnostics is the high-quality and interpretable classification of audio signals. In this study, we present an image-based representation of infant crying audio files to predict abnormal infant cries using a vision transformer and also show significant improvements in the performance and interpretability of this computer-aided tool. The use of advanced feature extraction techniques such as Gammatone Frequency Cepstral Coefficients (GFCCs) resulted in a classification accuracy of 96.33%. For other features (spectrogram and mel-spectrogram), the performance was very similar, with an accuracy of 93.17% for the spectrogram and 94.83% accuracy for the mel-spectrogram. We used our vision transformer (ViT) model, which is less complex but more effective than the proposed audio spectrogram transformer (AST). We incorporated explainable AI (XAI) techniques such as Layer-wise Relevance Propagation (LRP), Local Interpretable Model-agnostic Explanations (LIME), and attention mechanisms to ensure transparency and reliability in decision-making, which helped us understand the why of model predictions. The accuracy of detection was higher than previously reported and the results were easy to interpret, demonstrating that this work can potentially serve as a new benchmark for audio classification tasks, especially in medical diagnostics, and providing better prospects for an imminent future of trustworthy AI-based healthcare solutions.
Enhancing Stability and Efficiency in Mobile Ad Hoc Networks (MANETs): A Multicriteria Algorithm for Optimal Multipoint Relay Selection
Authors: Abdellaoui Ayoub , Himeur Yassine , Alnaseri Omar , Atalla Shadi , Mansoor Wathiq , Elmhamdi Jamal , Al-Ahmad Hussain
Published: November 2024

Mobile ad hoc networks (MANETs) are autonomous systems composed of multiple mobile nodes that communicate wirelessly without relying on any pre-established infrastructure. These networks operate in highly dynamic environments, which can compromise their ability to guarantee consistent link lifetimes, security, reliability, and overall stability. Factors such as mobility, energy availability, and security critically influence network performance. Consequently, the selection of paths and relay nodes that ensure stability, security, and extended network lifetimes is fundamental in designing routing protocols for MANETs. This selection is pivotal in maintaining robust network operations and optimizing communication efficiency. This paper introduces a sophisticated algorithm for selecting multipoint relays (MPRs) in MANETs, addressing the challenges posed by node mobility, energy constraints, and security vulnerabilities. By employing a multicriteria-weighted technique that assesses the mobility, energy levels, and trustworthiness of mobile nodes, the proposed approach enhances network stability, reachability, and longevity. The enhanced algorithm is integrated into the Optimized Link State Routing Protocol (OLSR) and validated through NS3 simulations, using the Random Waypoint and ManhattanGrid mobility models. The results indicate superior performance of the enhanced algorithm over traditional OLSR, particularly in terms of packet delivery, delay reduction, and throughput in dynamic network conditions. This study not only advances the design of routing protocols for MANETs but also significantly contributes to the development of robust communication frameworks within the realm of smart mobile communications.
Exploring the Adoption of Robotics in Teaching and Learning in Higher Education Institutions
Authors: Phokoye Samkelisiwe Purity , Epizitone Ayogeboh , Nkomo Ntando , Mthalane Peggy Pinky , Moyane Smangele Pretty , Khumalo Mbalenhle Marcia , Luthuli Mthokozisi , Zondi Nombuso Phamela
Published: November 2024

Artificial intelligence (AI) has become a prevalent part of many businesses, including higher education. AI is progressively gaining traction as an instrumental engagement tool in higher education institutions (HEIs). The premise underlying this trend is the potential of robots to foster enhanced student engagement and, consequently, elevate academic performance. Considering this development, HEI’s must probe deeper into the possible adoption of robotics in educational practices. This paper aims to conduct a comprehensive exploration into the adoption of robotics in teaching and learning in the higher education space. To provide a holistic perspective, this study poses three questions: what factors influence robotics uptake in HEIs, how can robots be integrated to improve teaching and learning in HEIs, and what are the perceived benefits of robotics implementation in teaching and learning. A bibliometric analysis and comprehensive review methodology were employed in this study to provide an in-depth assessment of the development, significance, and implications of robotics in HEIs. The dual approach offers a robust evaluation of robotics as a pivotal element needed for the enhancement of teaching and learning practices. The study’s findings uncover the increasing adoption of robotics within the higher education sphere. It also identifies the challenges encountered during adoption, ranging from technical hurdles to educational adjustments. Furthermore, this paper offers guidelines for various stakeholders for the effective integration of robotics into higher education.
Further Evidence That Female Anoplophora glabripennis (Coleoptera: Cerambycidae) Utilizes Photo-Degradation to Produce Volatiles That Are Attractive to Adult Males
Authors: Crook Damon , Wickham Jacob , Ren Lili , Xu Zhichun , Jones Tappey H. , Warden Melissa , Cossé Allard
Published: November 2024

The Asian longhorned beetle, Anoplophora glabripennis (Coleoptera: Cerambycidae: Lamiinae), is a serious pest of over 43 species of hardwood trees in North America, China and Europe. The development of an effective lure and trap for monitoring A. glabripennis has been hindered by the fact that mate finding involves a rather complex series of behaviors and responses to several chemical (and visual), cues. Adults (female-biased) locate a tree via host kairomones. Research has demonstrated that female contact pheromone components are precursors that undergo abiotic oxidation to yield attractive volatile components. Males also produce a pheromone to attract other adults nearby before the final step of recognition by males to the female-produced trail and contact pheromones. Our research aimed to identify new female-produced components from ozone and UV-treated A. glabripennis body washes and test them for behavioral activity using laboratory and field assays. The ozone and UV treatment of virgin female extract yielded sixteen aldehydes, nine of which were found in trace amounts. All sixteen aldehydes elicited antennal responses (GC-EAD) in both males and females, although responses were clearer and more distinct with standards of hexanal, heptanal, octanal, nonanal, decanal, undecanal and dodecanal. Olfactometer assays showed that males were highly attracted to a blend of these seven aldehydes. Females did not show any attraction to the blend in olfactometer assays. Despite low population levels during field tests in 2018 in China, traps containing the aldehyde blend detected A. glabripennis on a weekly basis over 6 weeks and caught significantly more adults (mainly males) than control ‘flight intercept panel’ traps. Field tests in China in 2019 that used the aldehyde blend along with a three-component host blend lure also caught significantly more males (nearly 5×) than blank control traps.
Disparities in Advance Care Planning Across Rurality, Sociodemographic Characteristics, and Cognition Levels: Evidence from the Health and Retirement Study
Authors: Rahemi Zahra , Bacsu Juanita-Dawne R. , Shalhout Sophia Z. , Sabet Morteza , Sirizi Delaram , Smith Matthew Lee , Adams Swann Arp
Published: November 2024

Background: We aimed to examine ACP in older adults in the U.S. across different sociodemographic characteristics and cognition levels (N = 17,698). Methods: We utilized two legal documents from the Health and Retirement Study survey: a living will and durable power of attorney for healthcare (DPOAH). We established the baseline trends from 2014 to assess if trends in 2024 have improved upon future data availability. Logistic regression models were fitted with outcome variables (living will, DPOAH, and both) stratified by cognition levels (dementia/impaired cognition versus normal cognition). Results: Age, ethnicity, race, education, and rurality were significant predictors of ACP (having a living will, DPOAH, and both the living will and DPOAH) across cognition levels. Participants who were younger, Hispanic, black, less educated, or resided in rural areas were less likely to complete ACP. Conclusion: Examining ACP and its linkages to specific social determinants is crucial for understanding disparities and developing effective educational and interventional strategies to enhance ACP uptake among diverse population groups. Future studies are needed to assess whether disparities have improved over the last decade, particularly as 2024 data become available. Addressing ACP disparities is essential for healthcare professionals to advance research and promote effective practices in geriatric care and aging services.
Single-Centre Analysis of Magnetic Resonance Imaging of Sacroiliac Joints in a Paediatric Population
Authors: Ożga Joanna , Ostrogórska Monika , Wojciechowski Wadim , Żuber Zbigniew
Published: November 2024

Background: Sacroiliitis in children is usually connected with one of the subtypes of juvenile idiopathic arthritis (JIA), such as enthesitis-related arthritis, psoriatic arthritis, or undifferentiated arthritis. The main diagnostic method is magnetic resonance imaging (MRI) of the sacroiliac joints, which can reveal bone marrow edema (BME) as a sign of an active inflammation process. This research aimed to retrospectively investigate the associations between the clinical presentation, laboratory test results, and MRI results of the sacroiliac joints of children. Methods: A total of 152 paediatric patients who underwent MRI of the sacroiliac joints were included in this single-centre study. The mean age of patients was 13.91 ± 2.97, while the female-to-male ratio was 1.36:1. JIA diagnosis was confirmed in 91 (59.87%) patients. Results: The main symptom reported by 128 (83.21%) patients was chronic pain, while in another 40 (31.25%) patients, it was chronic back pain. Patients with arthritis and BME in the sacroiliac joints were more likely to report chronic back pain, while patients with JIA but without BME in the sacroiliac joints were often positive for anti-nuclear antibodies (ANA). The widening of any joint contour was observed in 43 (28.29%) patients, and reduced joint mobility was observed in 61 (40.13%). Elevation of inflammatory blood parameters occurred in 31 (20.39%) patients, but this was not statistically related to BME presence in the sacroiliac joints. Radiological findings included BME (n = 36; 23.68% of examinations), joint space narrowing (n = 10; 27.78% of examinations), erosions (n = 7; 19.44% of examinations), and joint fluid (n = 7; 19.44% of examinations). There was a statistically significant relationship between the presence of BME in the sacroiliac joints and all of the above radiological findings. Conclusions: The radiological findings of MRI of the sacroiliac joints are significantly statistically related to chronic back pain in patients, while there is no relationship between any inflammatory blood parameter and the presence of BME.