bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and validation of SHC1 and FGFR1 as novel immune-related oxidative stress biomarkers of non-obstructive azoospermia
Authors: Pan Yang , Chen Xiangyu , Zhou Hang , Xu Mingming , Li Yuezheng , Wang Qihua , Xu Zhunan , Ren Congzhe , Liu Li , Liu Xiaoqiang
Published: September 2024

Background

Non-obstructive azoospermia (NOA) is a major contributor of male infertility. Herein, we used existing datasets to identify novel biomarkers for the diagnosis and prognosis of NOA, which could have great significance in the field of male infertility.

Methods

NOA datasets were obtained from the Gene Expression Omnibus (GEO) database. CIBERSORT was utilized to analyze the distributions of 22 immune cell populations. Hub genes were identified by applying weighted gene co-expression network analysis (WGCNA), machine learning methods, and protein–protein interaction (PPI) network analysis. The expression of hub genes was verified in external datasets and was assessed by receiver operating characteristic (ROC) curve analysis. Gene set enrichment analysis (GSEA) was applied to explore the important functions and pathways of hub genes. The mRNA–microRNA (miRNA)–transcription factors (TFs) regulatory network and potential drugs were predicted based on hub genes. Single-cell RNA sequencing data from the testes of patients with NOA were applied for analyzing the distribution of hub genes in single-cell clusters. Furthermore, testis tissue samples were obtained from patients with NOA and obstructive azoospermia (OA) who underwent testicular biopsy. RT-PCR and Western blot were used to validate hub gene expression.

Results

Two immune-related oxidative stress hub genes (SHC1 and FGFR1) were identified. Both hub genes were highly expressed in NOA samples compared to control samples. ROC curve analysis showed a remarkable prediction ability (AUCs > 0.8). GSEA revealed that hub genes were predominantly enriched in toll-like receptor and Wnt signaling pathways. A total of 24 TFs, 82 miRNAs, and 111 potential drugs were predicted based on two hub genes. Single-cell RNA sequencing data in NOA patients indicated that SHC1 and FGFR1 were highly expressed in endothelial cells and Leydig cells, respectively. RT-PCR and Western blot results showed that mRNA and protein levels of both hub genes were significantly upregulated in NOA testis tissue samples, which agree with the findings from analysis of the microarray data.

Conclusion

It appears that SHC1 and FGFR1 could be significant immune-related oxidative stress biomarkers for detecting and managing patients with NOA. Our findings provide a novel viewpoint for illustrating potential pathogenesis in men suffering from infertility.
Investigating the role and regulation of GPNMB in progranulin-deficient macrophages
Authors: Gillett Drew A. , Neighbarger Noelle K. , Cole Cassandra , Wallings Rebecca L. , Tansey Malú Gámez
Published: September 2024

Introduction

Progranulin (PGRN) is a holoprotein that is internalized and taken to the lysosome where it is processed to individual granulins (GRNs). PGRN is critical for successful aging, and insufficient levels of PGRN are associated with increased risk for developing neurodegenerative diseases like AD, PD, and FTD. A unifying feature among these diseases is dysregulation of peripheral immune cell populations. However, considerable gaps exist in our understanding of the function(s) of PGRN/GRNs in immune cells and their role in regulating central-peripheral neuroimmune crosstalk. One of the most upregulated genes and proteins in humans with GRN haploinsufficiency and in aged Grn knock-out (KO) mice is glycoprotein non-metastatic B (GPNMB) but its normal role within the context of immune crosstalk has not been elucidated.

Methods

To address this gap, peritoneal macrophages (pMacs) from 5-to-6-month old WT and Grn KO mice were assessed for Gpnmb expression and stimulation-dependent cytokine release in the presence or absence of the Gpnmb extracellular domain (ECD). Cellular localization, as well as inhibition of, the microphthalmia-associated transcription factor (MITF) was assessed to determine its mechanistic role in Gpnmb overexpression in Grn KO pMacs.

Results

We observed an increase in GPNMB protein and mRNA as a result of insufficient progranulin in peripheral immune cells at a very early age relative to previous reports on the brain. Stimulation-dependent cytokine release was decreased in the media of Grn KO pMacs relative to WT controls; a phenotype that could be mimicked in WT pMacs with the addition og GPNMB ECD. We also found that MITF is dysregulated in Grn KO pMacs; however, its nuclear translocation and activity are not required to rescue the immune dysregulation of Grn KO macrophages, suggesting redundancy in the system.

Discussion

These findings highlight the fact that knowledge of early-stage disease mechanism(s) in peripheral populations may inform treatment strategies to delay disease progression at an early, prodromal timepoint prior to development of neuroinflammation and CNS pathology.
Low-dose aspirin and heparin treatment improves pregnancy outcome in recurrent pregnancy loss women with anti-β2-glycoprotein I/HLA-DR autoantibodies: a prospective, multicenter, observational study
Authors: Tanimura Kenji , Saito Shigeru , Tsuda Sayaka , Ono Yosuke , Deguchi Masashi , Nagamatsu Takeshi , Fujii Tomoyuki , Nakatsuka Mikiya , Kobashi Gen , Arase Hisashi , Yamada Hideto
Published: September 2024

Introduction

Anti-β2-glycoprotein I (β2GPI)/human leukocyte antigen (HLA)-DR antibodies may be a risk factor for recurrent pregnancy loss (RPL). The therapeutic modality for women with RPL and anti-β2GPI/HLA-DR antibody positivity has not been evaluated. This prospective, multicenter, observational study aimed to assess whether low-dose aspirin (LDA) and/or heparin therapies improve pregnancy outcomes in women with RPL who tested positive for anti-β2GPI/HLA-DR antibodies.

Methods

Between August 2019 and December 2021, 462 women with RPL underwent anti-β2GPI/HLA-DR antibody measurements and risk assessments for RPL. Each attending physician decided the treatment modality for women with RPL who tested positive for anti-β2GPI/HLA-DR antibodies, and their pregnancy outcomes were followed up until December 2023. Finally, 47 pregnancies in 47 women with RPL and anti-β2GPI/HLA-DR antibody positivity were included in the analysis and were divided into two groups regarding whether they were treated with LDA and/or unfractionated heparin (UFH) (LDA/UFH group, n = 39) or with neither of them (non-LDA/non-UFH group, n = 8). The rates of live birth and pregnancy complications (i.e., preeclampsia and preterm delivery before 34 gestational weeks due to placental insufficiency) were compared between the two groups.

Results

The live birth rate in the LDA/UFH group was higher than that in the non-LDA/non-UFH group (87.2% vs 50.0%, p = 0.03). The pregnancy complication rate in the LDA/UFH group was significantly lower than that in the non-LDA/non-UFH group (5.9% vs 50.0%, p = 0.048). Among 21 women who tested positive for anti-β2GPI/HLA-DR antibodies and had no other risk factors for RPL, the live birth rate in the LDA/UFH group (n = 14) was much higher than that in the non-LDA/non-UFH group (n = 7) (92.9% vs 42.9%, p = 0.03).

Discussion

This study, for the first time, demonstrated that LDA and/or UFH therapies are effective in improving pregnancy outcomes in women with RPL and aβ2GPI/HLA-DR antibody positivity.
Acute leukoencephalopathy associated with daratumumab treatment in POEMS syndrome: a case report
Authors: Steinegger Lukas , Nierobisch Nathalie , De Vere-Tyndall Anthony , Schreiner Bettina , Roth Patrick , Kappos Ludwig , Kana Veronika , Herwerth Marina
Published: September 2024

Objectives

Daratumumab, a monoclonal antibody against CD38, is increasingly used in the treatment of multiple myeloma, other hematological malignancies and autoimmune diseases. Little is known about its CNS toxicity. We present a case of a patient with POEMS syndrome (syndrome of polyneuropathy, organomegaly, endocrinopathy, monoclonal gammopathy and skin changes) who developed an acute leukoencephalopathy shortly after initiation of therapy with daratumumab.

Methods

Case report following the CARE case report guidelines

Results

The patient presented with symptoms of headache and diffuse worsening of a pre-existing tetraparesis. MRI showed a rapidly progressive leukoencephalopathy. Extensive diagnostic evaluation revealed no specific cause, suggesting the leukoencephalopathy to be caused by daratumumab.

Discussion

Our report highlights a probably rare, but clinically significant adverse effect of daratumumab and underlines the necessity of raised vigilance for neurological side effects in patients treated with daratumumab.
The gut homeostasis-immune system axis: novel insights into rheumatoid arthritis pathogenesis and treatment
Authors: Qi Peng , Chen Xin , Tian Jiexiang , Zhong Kexin , Qi Zhonghua , Li Menghan , Xie Xingwen
Published: September 2024

Rheumatoid arthritis is a widely prevalent autoimmune bone disease that imposes a significant burden on global healthcare systems due to its increasing incidence. In recent years, attention has focused on the interaction between gut homeostasis and the immune system, particularly in relation to bone health. Dysbiosis, which refers to an imbalance in the composition and function of the gut microbiota, has been shown to drive immune dysregulation through mechanisms such as the release of pro-inflammatory metabolites, increased gut permeability, and impaired regulatory T cell function. These factors collectively contribute to immune system imbalance, promoting the onset and progression of Rheumatoid arthritis. Dysbiosis induces both local and systemic inflammatory responses, activating key pro-inflammatory cytokines such as tumor necrosis factor-alpha, Interleukin-6, and Interleukin-17, which exacerbate joint inflammation and damage. Investigating the complex interactions between gut homeostasis and immune regulation in the context of Rheumatoid arthritis pathogenesis holds promise for identifying new therapeutic targets, revealing novel mechanisms of disease progression, and offering innovative strategies for clinical treatment.
The gut-eye axis: the retinal/ocular degenerative diseases and the emergent therapeutic strategies
Authors: Kammoun Sonda , Rekik Mona , Dlensi Aryj , Aloulou Samir , Smaoui Walid , Sellami Sahla , Trigui Khaled , Gargouri Rahma , Chaari Imen , Sellami Hayet , Elatoui Dhawia , Khemakhem Nahed , Hadrich Ines , Neji Sourour , Abdelmoula Balkiss , Bouayed Abdelmoula Nouha
Published: September 2024

The interplay between human microbiota and various physiological systems has garnered significant attention in recent years. The gut microbiota plays a critical role in maintaining physiological homeostasis and influences various aspects of human health, particularly via the gut brain axis. Since 2017, the challenging concept of the gut-retina axis has emerged thanks to a network analysis emphasizing the potential role of the gut microbiota disruption in the development of the age-related macular degeneration and further retinal damages. Many other ocular disorders have been linked to the dysbiosis of the gut microbiota, including uveitis and glaucoma. It has been shown that age related macular degeneration can be prevented or reversed using a diet that induces changes in the gut microbiota. The potential link between the gut microbiota as well as others types of microbiota such as the ocular surface microbiota and the development/progression of age related as well as inherited retinal degenerations and other degenerative eye diseases, has recently been broadened. Therefore, the pathogenesis of several eye diseases has recently been associated with a larger perception called the gut eye axis. This mini-review examines the potential mechanisms underlying the gut eye axis and suggests implications for the management of eye diseases. By understanding the modulation of the gut microbiota and its impact on eye disease, this mini-review provides insight into potential therapeutic interventions and avenues for future research.
Robot-assisted therapy in stratified intervention: a randomized controlled trial on poststroke motor recovery
Authors: Liu Yang , Cui Lijun , Wang Jixian , Xiao Zihao , Chen Zhi , Yan Jin , Niu Chuanxin M. , Xie Qing
Published: September 2024

Objective

To compare the effects of robot-assisted therapy with conventional therapy for accelerating stratified intervention in poststroke patients with upper limb dysfunction.

Background

For stroke survivors, recovery of upper extremity function remains a major challenge in rehabilitation. Literature has suggested that the rate of recovery may improve if treatments can be individualized to their clinical profiles. However, there still lack clinical evidence on how to create treatment tailored to individual patients. Robot-assisted Therapy (RT) provides a straightforward approach to adjustment of the assistance-resistance continuum for individual patients. In early Brunnstrom stages of recovery, patients benefit from assistance training, whereas in later stages the training is favored with resistance. Therefore, RT may enhance Conventional Therapy (CT) but the use of RT in stratified intervention has not been investigated. This study evaluated the possible benefit of adopting RT following a protocol of upper-limb training, which was stratified with the Brunnstrom stage of each individual.

Methods

This study was a single-blinded randomized controlled trial. A total of 53 patients with stroke were recruited and randomized into 2 groups (CT, n = 27, 3 dropped out and RT, n = 26, 2 dropped out). Both groups were trained once per day, 5 days per week for 4 weeks. The CT group received 30 min of conventional therapy; the RT group received 30 min of upper limb robot-assisted training. Patients were assessed at the beginning, week-2, and week-4 of the treatment. The outcome measures included the Fugl-Meyer Assessment Upper-Extremity (FMA-UE) and the Modified Barthel Index (MBI).

Results

Across the 4-week intervention, participants in the RT group recovered 1.979 points of FMA-UE per week, compared to 1.198 points per week in the CT group (t94 = 3.333, p < 0.01); the recovery rate was 0.781 points/week higher in the RT group than in the CT group. Moreover, the recovery of FMA-UE was faster in proximal joints (t94 = 3.199, p < 0.01), and for patients in Brunnstrom Stage III (t34 = 2.526, p < 0.05). The improvements in MBI were not significantly different between RT and CT.

Conclusion

Robot-assisted therapy showed initial evidence for the acceleration of post-stroke recovery of motor function in the upper limb. Initial observations suggested that patients in Brunnstrom recovery stage III might benefit the most from the stratified intervention assisted by robotics.

Clinical trial registration

https://www.chictr.org.cn/showproj.html?proj=61834, Identifier [ChiCTR2000039010]. Registered 13 March 2020.
Nutritional status and dietary habits in older adults with fixed implant dental prostheses: a case-control study
Authors: Homsi George , Trulsson Mats , Grigoriadis Anastasios , Kumar Abhishek
Published: September 2024

Aim

To evaluate the nutritional status, nutritional risk, and dietary habits of patients treated with bimaxillary implant-supported fixed prostheses in comparison with a group of natural dentate patients.

Methods

A study group (n = 25, 8 women, mean age = 70.6 ± 7.5 years) with bimaxillary implant-supported fixed prostheses and a control group (n = 25, 13 women, mean age = 69.0 ± 5.3) with a mean of 27.7 ± 1.8 natural teeth were recruited. The nutritional status and nutritional risk of the participants were evaluated with Mini Nutritional Assessment (MNA) and Seniors in the Community: Risk Evaluation for Eating and Nutrition; (SCREEN-14), while the dietary habits were recorded by data from a three-day dietary record. The data were analyzed with the Mann–Whitney U-test and independent t-test to evaluate the differences between the groups.

Results

The results showed that although both the groups had normal nutrition status as revealed by the MNA scores the study group showed significantly higher BMI (p = 0.005) but lower SCREEN-14 (p = 0.012) scores, than the control group. The results also showed that higher SCREEN-14 scores were significantly associated with higher odds of being in the control group, with an odds ratio of 1.159 (p = 0.024). Further, the results of the analysis of the dietary records showed that the participants in the study group consumed fewer meals (p = 0.006) and fewer varieties of food (p < 0.001), particularly fewer fruits (p = 0.011) than the control group.

Conclusion

The results indicate that people with fixed implant prostheses may be susceptible to nutritional deficiencies according to the SCREEN-14 scores compared to their natural dentate counterparts. Further, people with implant prostheses also tend to have higher BMI and consume a smaller variety of foods, especially fruits, than the natural dentate control group.
Value of the lung immune prognostic index in patients with advanced small cell lung cancer treated with programmed death-ligand 1 and programmed death-1 inhibitors in the Chinese alpine region
Authors: Zhang Meiling , Hao Jingwei , Wu Yunjiao , Gao Ziyi , Wang Meng
Published: September 2024

Purpose

To assess the potential added value of the lung immune prognostic index (LIPI) in patients with small cell lung cancer (SCLC), treated with programmed death-ligand 1 (PD-L1)/programmed death-1 (PD-1) inhibitors, who lived in the Chinese alpine region.

Methods

120 SCLC patients treated with PD-L1/PD-1 inhibitors were divided into three LIPI groups, from July 2018 to April 2021. Cox regression models were used to evaluate the prognostic effect of three LIPI groups on overall survival (OS) and progression-free survival (PFS). Logistic regression analysis was conducted to explore the association between immune-related adverse events (irAEs) and the pretreatment of neutrophil-to-lymphocyte ratio (dNLR), lactate dehydrogenase (LDH), and LIPI.

Results

The median OS was 4.5, 6.3, and 10.0 months (p=0.001) and the median PFS was 2.5, 4.3, and 5.3 months (p=0.049) for Poor, Intermediate, and Good LIPI, respectively. The disease control rate (DCR) was also higher in the Good LIPI group (p=0.003). Moreover, multivariate analysis confirmed that worse LIPI was correlated with shorter OS and PFS. dNLR was associated with the onset of irAEs, not LIPI. Conclusion: The LIPI might be a promising predictive and prognostic biomarker in SCLC patients treated with PD-L1/PD-1 inhibitors in the Chinese Alpine region.
Spillover of sustainable routines from work to private life: application of the Identity and Practice Interdependence Framework
Authors: Frezza Marcia
Published: September 2024

Introduction

Spillover of sustainable routines and environmentally-responsible behaviors from one setting to another may contribute to achieving essential sustainability goals. Several previous studies on this topic have found few examples of spillover and have focused primarily on individual influences, indicating the need for a better understanding of the factors that have an impact on whether, how, and when spillover occurs. To this end, a novel conceptualization examining the interactions between identity principles and socio-material elements, the Identity and Practice Interdependence Framework, was applied to investigate the occurrence of spillover of sustainable routines from the workplace to home.

Methods

Three focus groups totaling 30 employees of a major Brazilian steel-producing company, interviews in 15 employee homes, and on-site observations of work and private domains provided data that was analyzed qualitatively, using a deductive reflexive thematic approach.

Results

Participants identified 58 changes in home routines related to sustainability as resulting from their experiences at work. With the consistent availability of practice elements (materials, competencies, meanings), learning about, witnessing and performing sustainable routines at work favored satisfactory levels of identity principles (self-efficacy, self-esteem, distinctiveness, continuity). Analysis suggested that seeking the same satisfactions of identity principles outside the workplace led individuals to adapt, change, and/or create more environmentally-responsible routines in their homes.

Discussion

The Identity and Practice Interdependence Framework permitted investigation of the integration of socio-material aspects with the role of individuals in the process of spillover, and indicated some ways they may interact. Both the provision of socio-material components in the first setting and the recognition of more sustainable routines as a feasible path to satisfaction of identity principle needs contribute to individual engagement and persistence in the second setting. Consistent and frequent experiences with more sustainable routines in the first setting or situation may be key to creating this expectation, and therefore to the occurrence of spillover to another setting. The framework complements previous approaches by allowing for a more complex analysis of spillover, which can be used to enrich research on sustainable practices and help promote environmentally-friendly behaviors and sustainable routines, or other desired outcomes, both within organizations and beyond.
Tools for screening maternal mental health conditions in primary care settings in sub-Saharan Africa: systematic review
Authors: Gyimah Leveana , Agyepong Irene Akua , Owiredu David , Awini Elizabeth , Yevoo Linda Lucy , Ashinyo Mary Eyram , Aye Sorre Grace Emmanuelle Victoire , Abbas Shazra , Cronin de Chavez Anna , Mirzoev Tolib , Danso-Appiah Anthony
Published: September 2024

Introduction

In sub-Saharan Africa, pregnant and postpartum women with mental health problems are often missed in healthcare systems. To address this, a practical and simple screening tool for maternal mental health should be available to primary healthcare workers. An important step toward having such a tool is to assess the existing tools and their effectiveness in primary care settings.

Methods

We systematically searched PubMed, LILAC, CINAHL, Google Scholar, African Index Medicus, HINARI, and African Journals Online from inception to 31 January 2023, without language restriction. Reference lists of retrieved articles were reviewed and experts in the field were contacted for studies not captured by our searches. All retrieved records were collated in Endnote, de-duplicated, and exported to Rayyan for screening. Study selection and data extraction were done by at least two reviewers using a pre-tested flow chart and data extraction form. Disagreements between reviewers were resolved through discussion. We contacted primary authors for missing or insufficient information and conducted a content analysis of the psychometric properties of the tools.

Results

In total, 1,181 studies were retrieved by our searches, of which 119 studies were included in this review. A total of 74 out of 119 studies (62%) were screened for depression during pregnancy and or the postpartum period. The Edinburg Postpartum Depression Scale (EPDS) and the Patient Health Questionnaire (PHQ-9) were the most commonly used tools. In total, 12 studies reported specificity and sensitivity for tools for measuring depression (EPDS, PHQ-9, and Whooley) and psychological distress [Self Report Questionnaire (SRQ) and Kessler Psychological Distress Scale (KPDS)]. The average sensitivity and specificity of the EPDS reported were 75.5 and 76.5%, respectively, at a cut-off of ≥13. The EPDS appears to be the most acceptable, adaptable, user-friendly, and effective in screening for maternal mental health conditions during pregnancy and postpartum. However, the methodological approach varied for a particular tool, and documentation on the attributes was scanty.

Conclusion

The EPDS was the most commonly used tool and considered as most acceptable, adaptable, user-friendly, and effective. Information on the performance and psychometric properties of the vast majority of screening tools was limited.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022323558, identifier CRD42022323558 (PROSPERO).
The mediating role of social connectedness and negative cognitive emotion regulation in the association between problematic Internet use and depression among adolescents
Authors: Xu Jiaqi , Chen Xia-Can , Chen Lihua , Luo Dan , Bao Wenxin , Yang Xia , Ran Junzhe , Xu Jiajun
Published: September 2024

Introduction

While the relationship between adolescent problematic Internet use (PIU) and depression has been extensively researched, few studies have investigated the role and mechanisms of social connectedness (SC) in this context. This study aimed to investigate the mediating effect of social connectedness (SC) and cognitive emotion regulation (CER) in the relationship between PIU and depression.

Methods

We conducted a cross-sectional study involving 9,407 adolescents aged 12–18 years in China from September 2022 to March 2023. We employed Young’s 20-item Internet Addiction Test (IAT-20), the Social Connectedness Scale-Revised (SCS-R), the Cognitive Emotion Regulation Scale (CER), and the DSM-5 Level-2 Depression Scale to assess mental health outcomes. Logistic regression analysis was also performed to examine the independent association between the measured variables and depression. Mediation analysis was then conducted to evaluate the mediating roles of social connectedness and cognitive emotion regulation in the relationship between PIU and depression.

Results

We found that the prevalence of PIU was 21.8%. Offline SC (indirect effect: 0.112, 95% CI: 0.104–0.121) and negative CER (indirect effect: 00.140, 95% CI: 0.129–0.152) mainly played a parallel mediating role in the relationship between PIU and depression, along with online SC (on_SC) (indirect effect: 0.007, 95% CI: 0.005–0.010).

Discussion

These findings provide valuable insights into how PIU is associated with depression and highlight the importance of fostering real-life interpersonal interactions. However, the generalizability of this study’s findings to other populations may be limited due to cultural factors.
Intergenerational education mobility in India: nonlinearity and the Great Gatsby Curve
Authors: Kishan P. K. V. , Rajverma Abhinav
Published: September 2024

Introduction

Intergenerational education mobility, which reflects the degree to which an individual’s educational attainment is independent of their parents’ education, is essential for promoting equal opportunities in society. In the context of India, where socio-economic disparities are deeply entrenched, understanding the dynamics of intergenerational mobility is particularly crucial.

Methods

This paper examines various aspects of intergenerational education mobility in India using data from the Indian Human Development Survey (IHDS), a nationally representative multi-topic survey. We analyze intergenerational mobility across different age cohorts and investigate the nonlinearities in the transmission of education. Additionally, we explore the impact of educational inequality, economic growth, and public expenditure on education on mobility outcomes.

Results

Our analysis reveals a high degree of intergenerational persistence in education, although this persistence has decreased over time. Employing quantile regressions, we observe significant nonlinearities in the relationship between fathers’ and sons’ educational outcomes across the educational distribution. In particular, we find a widening mobility gap between historically advantaged subgroups (urban populations, upper castes, Hindus) and disadvantaged groups (rural populations, lower castes, Muslims) at the middle and upper quantiles. Moreover, we confirm the “Higher Inequality leading to Lesser Mobility” nexus, supporting the ‘Great Gatsby Curve’ within the Indian context. Macroeconomic factors, such as economic growth and public expenditure on education, are positively correlated with educational mobility, suggesting that these factors play a critical role in enhancing mobility.

Discussion

These findings highlight the importance of targeted policy interventions to reduce educational disparities and promote greater intergenerational mobility. The widening mobility gaps between socio-economic and demographic groups emphasize the need for more equitable resource distribution and educational reforms. Future research should explore the multifaceted aspects of intergenerational mobility, incorporating longitudinal studies and regional analyses to deepen our understanding of the underlying mechanisms.
Total hepatic inflow occlusion vs. hemihepatic inflow occlusion for laparoscopic liver resection: a systematic review and meta-analysis
Authors: An Ting , Liu Jie , Feng Liwei
Published: September 2024

The control of bleeding during laparoscopic liver resection (LLR) is still a focus of research. However, the advantages of the main bleeding control methods, including total hepatic inflow occlusion (TIO) vs. hemihepatic inflow occlusion (HIO), during LLR remain controversial. The purpose of this meta-analysis was to compare the clinical outcomes of patients who received TIO and patients who received HIO. This meta-analysis searched the Medline, PubMed, Web of Science, Embase, Ovid, and Cochrane Library databases. The language of the studies was restricted to English, and comparative studies of patients treated with TIO and HIO during LLR were included. The primary outcome was to compare the intraoperative details, such as the operative time, occlusion time, and volume of blood loss, between the two groups. Secondary outcomes included conversion, overall complications, liver failure, biliary leakage, ascites, pleural effusion, and hospital stay. Five studies including 667 patients, 419 (62.82%) of whom received TIO and 248 (37.18%) of whom received HIO, were included in the analysis. The demographic data, including age, sex, hemoglobin, total bilirubin, albumin, and alpha-fetoprotein, were comparable. No significant differences noted in operative time, occlusion time, volume of blood loss, conversion, overall complications, liver failure, biliary leakage, hemorrhage, ascites, or pleural effusion. The hospital stay in patients who received HIO was significantly shorter than that for patients who received TIO [mean difference (MD), 0.60; 95% confidence interval (CI), 0.33–0.87; p < 0.0001; I2 = 54%]. The blood loss of patients with liver cirrhosis in the TIO group was significantly less than that in the HIO group (MD, −107.63; 95% CI, −152.63 to −62.63; p < 0.01; I2 = 27%). Both the TIO and HIO methods are safe and feasible for LLR. Compared with HIO, TIO seems to have less blood loss in cirrhotic patients. However, this result demands further research, especially multicenter randomized controlled trials, for verification in the future.

Systematic Review Registration:https://www.crd.york.ac.uk/, Identifier PROSPERO (CRD42022382334).
Contamination of liposomal bupivacaine during multi-dose usage in a clinical setting evaluated by culture and PCR
Authors: Melvin Rachel L. , Wu Jing , Bennett Brad S. , Lawhon Sara D. , Savarino Amy , Rogovskyy Artem S. , Thieman Mankin Kelley M.
Published: September 2024

Introduction

Liposomal bupivacaine, a long-acting local anesthetic, is sold in single-dose vials at a cost of approximately $200/20 mL vial. As many veterinary patients are not dosed an entire vial, the vials have been used for multiple doses at our institution to provide cost savings. Multiple punctures of a vial can lead to increased opportunity for contamination of the contents. This study aims to describe our institutional procedure for multi-dose use of single-dose liposomal bupivacaine vials and to evaluate clinically utilized liposomal bupivacaine for bacterial and fungal contamination using molecular and bacteriological methods.

Methods

The first (Control) and last (Sample B) 0.5 mL from each vial were collected and submitted for bacterial and fungal PCR, anaerobic and aerobic bacterial culture, and opportunistic fungal culture.

Results

All 40 bacterial cultures yielded no growth; Bacterial or fungal DNA was identified in 19 samples (50%). Of the 19 samples in which bacterial or fungal DNA was identified, 10 (52.6%) were from Control, and 9 (47.4%) were from Sample B. PCR does not appear to be useful in detecting bacterial or fungal contamination from liposomal bupivacaine.

Discussion

Results support the aseptic handling protocol described in this article is successful in preventing detectable bacterial and fungal contamination of liposomal bupivacaine vials for up to 7 individual punctures and vials open for up to 5 days.
Gut feelings: gastrointestinal signs in French bulldogs undergoing spinal surgery
Authors: du Toit Michelle , Motta Luca
Published: September 2024

Introduction

The French bulldog (FBD) is a brachycephalic breed prone to several neurological conditions, of which intervertebral disc herniation (IVDH) is considerably prevalent. Gastrointestinal (GI) disease is a reported complication in dogs surgically treated for IVDH. The objective of this study was to describe GI signs and their outcome in FBDs surgically treated for IVDH.

Materials and methods

Data regarding the GI signs (vomiting, diarrhoea and regurgitation), their frequency and short-term outcome in FBDs surgically treated for IVDH (cervical, thoracolumbar or lumbar) between January 2017 and April 2023 were obtained from medical records at one institution. Categorical variables were compared using Fisher exact tests, and ordinal/continuous data between categorical groups using Kruskal-Wallis or Mann-Whitney tests.

Results

Ninety-seven FBDs were included for analysis. GI signs occurred in 74/97 (76.3%) FBDs while hospitalised, with 33.8% and 66.2% developing GI signs pre- and post-operatively, respectively. FBDs that developed GI signs had a mean of 4.9 episodes. Diarrhoea was the most common GI sign encountered (51/74) compared to regurgitation (38/74) and vomiting (22/74). Resolution of GI signs occurred within a mean of 2.2 days. Mean duration of hospitalisation post-surgery was 4.6 days in FBDs that developed GI signs versus 3.7 days in FBDs that did not (p = 0.033). Anaesthesia length was associated with developing GI signs (p=0.037). Neurological severity, neuroanatomical localisation and surgical procedure were not associated with development of GI signs (p = 0.42, p = 0.794 and p = 1, respectively).

Conclusion

GI signs were commonly encountered in FBDs surgically treated for IVDH and associated with length of anaesthesia and prolonged hospitalisation.
Comprehensive Study on the Potential of Domesticated Clones of Rosemary (Salvia rosmarinus Spenn.): Implications for Large-Scale Production and Waste Recovery in the Development of Plant-Based Agrochemicals
Authors: Ortiz de Elguea-Culebras Gonzalo , Melero-Bravo Enrique , Ferrando-Beneyto Tamara , Jordán María José , Cáceres-Cevallos Gustavo , Sánchez-Vioque Raúl
Published: September 2024

Rosemary is a versatile Mediterranean shrub valued for its culinary and medicinal uses, also finding applications as a food additive (E-392). This study explores the potential of rosemary for large-scale cultivation as well as the valorization of its distillation residue, which constitutes more than 95% of the total biomass. Rich in bioactive compounds, this solid waste represents a valuable opportunity to develop renewable plant-based products. This study monitored the agronomic adaptations of cultivated clones of rosemary and evaluated the essential oil and phenolic content. This study also evaluated the biological potential of the ethanolic extracts from the distilled residue as an antifungal, antioxidant, chelator, and biostimulant in model tests. Interestingly, the extracts showed substantial phenolic content, exhibiting strong antifungal activity, antioxidant capacity, and efficient metal chelation. Furthermore, all extracts also demonstrated promising biostimulant effects on rooting. Among the clones evaluated, Pina de Ebro stood out especially for its balanced adaptability, high essential oil yield, and outstanding phenolic content, along with uniform biological capacities among individual plants and plots. Therefore, this study highlights the potential of utilizing the entire rosemary plant, enhancing the overall profitability of the crop and meeting the growing demand for eco-friendly and renewable resources in the market.
Non-Destructive Seed Viability Assessment via Multispectral Imaging and Stacking Ensemble Learning
Authors: Chu Ye Rin , Jo Min Su , Kim Ga Eun , Park Cho Hee , Lee Dong Jun , Che Sang Hoon , Na Chae Sun
Published: September 2024

The tetrazolium (TZ) test is a reliable but destructive method for identifying viable seeds. In this study, a non-destructive seed viability analysis method for Allium ulleungense was developed using multispectral imaging and stacking ensemble learning. Using the Videometerlab 4, multispectral imaging data were collected from 390 A. ulleungense seeds subjected to NaCl-accelerated aging treatments with three repetitions per treatment. Spectral values were obtained at 19 wavelengths (365–970 nm), and seed viability was determined using the TZ test. Next, 80% of spectral values were used to train Decision Tree, Random Forest, LightGBM, and XGBoost machine learning models, and 20% were used for testing. The models classified viable and non-viable seeds with an accuracy of 95–91% on the K-Fold value (n = 5) and 85–81% on the test data. A stacking ensemble model was developed using a Decision Tree as the meta-model, achieving an AUC of 0.93 and a test accuracy of 90%. Feature importance and SHAP value assessments identified 570, 645, and 940 nm wavelengths as critical for seed viability classification. These results demonstrate that machine learning-based spectral data analysis can be effectively used for seed viability assessment, potentially replacing the TZ test with a non-destructive method.
The Characteristics of Milk Fatty Acid Profile Predicted by Fourier-Transform Mid-Infrared Spectroscopy (FT-MIRS) in Chinese Holstein Cows
Authors: Li Chunfang , Wang Haitong , Fan Yikai , Zhou Zengpo , Li Yuanbao , Liang Shengchao , Ma Yabin , Zhang Shujun
Published: September 2024

Fatty acid is an important factor affecting the nutritional quality of milk. In this study, we collected and assessed 78,086 milk samples from 12,065 Chinese Holstein cows from 11 farms in Northern China from November 2019 to September 2022. The contents of eight fatty acid groups were predicted using FT-MIRS-based models. The contents of TFAs, SFAs, UFAs, MUFAs, PUFAs, and LCFAs in milk reached the highest at 96–125 DIM, and SCFA and MCFA contents reached the highest at 276–305 DIM. With the increase in somatic cell score, the contents of various fatty acid groups in milk gradually decreased, and the nutritional value of milk and flavor of dairy products gradually deteriorated. The contents of high-quality fatty acids in milk, particularly UFAs and MUFAs, were significantly higher in the non-pregnant state than in the pregnant state. However, SCFA and MCFA contents exhibited the opposite pattern. Our findings provided valuable information on the content and distribution range of fatty acid groups in milk from Chinese Holstein cows. Further analysis is warranted to explore the breeding of Chinese Holstein cows providing milk with abundant beneficial fatty acids.
Comparison between a Flash Glucose Monitoring System and a Portable Blood Glucose Meter for Monitoring of Cats with Diabetic Ketosis or Ketoacidosis
Authors: Eiermann Jennifer S. , Hazuchova Katarina , Vidondo Beatriz , Campos Miguel , Schuller Simone , Cui Yi
Published: September 2024

Cats with diabetic ketosis or ketoacidosis DK(A) require intensive glucose monitoring. The aim of this study was to assess the agreement between a portable blood glucose meter (PBGM) and a flash glucose monitoring system (FGMS; FreeStyle Libre 2.0 Abbott®) measuring interstitial glucose in cats with DK(A). Ten client-owned cats with naturally occurring DK(A) were prospectively enrolled. Glucose concentrations were assessed with both methods every 1–3 h until resolution of DK(A), and every 4–8 h thereafter. While the median FGMS measured glucose concentration (14.3 mmol/L) was significantly lower than the median PBGM measured glucose concentration (19 mmol/L) (p < 0.001), the overall correlation between the FGMS and PBGM was high (r = 0.88; p < 0.001). In the Parkes error grid analysis, 98.3% of measurements fell in zones A and B. Bland–Altman plot analysis demonstrated that in the low glycaemic range (BG < 5.5 mmol/L), 50% of FGMS measurements deviated more than ±0.83 mmol/L, and in the high glycaemic range (BG > 5.5 mmol/L), 81% of results deviated >15% from the PBGM measurements. There was significant inter-individual variation in the difference between glucose concentrations measured by the FGMS and PBGM (p < 0.001). In spite of being more easily tolerated and easier to use, currently this method cannot be recommended for routine monitoring of cats with DK(A).
Comparing the Performance of Automatic Milking Systems through Dynamic Testing Also Helps to Identify Potential Risk Factors for Mastitis
Authors: Milanesi Stefano , Donina Dario , Chierici Guido Viviana , Zaghen Francesca , Sora Valerio M. , Zecconi Alfonso
Published: September 2024

Automatic milking systems (AMSs) are revolutionizing the dairy industry by boosting herd efficiency, primarily through an increased milk yield per cow and reduced labor costs. The performance of milking machines, whether traditional or automated, can be evaluated using advanced vacuum meters through dynamic testing. This process involves scrutinizing the system and milking routine to identify critical points, utilizing the VaDia™ logger (BioControl AS, Rakkestad, Norway). Vacuum recordings were downloaded and analyzed using the VaDia Suite™ software under the guidance of a milking specialist. Access to data from AMSs across various manufacturers and herds facilitated a retrospective study aimed at describing and comparing key milk emission parameters for different AMS brands while identifying potential mastitis risk factors. Using the proper statistical procedures of SPSS 29.1 (IBM Corp., Armonk, NY, USA), researchers analyzed data from 4878 individual quarter milkings from cows in 48 dairy herds. Results indicated a significant variability in milking parameters associated with quarter milk yield and AMS brand. Notably, despite AMSs standardizing teat preparation and stimulation, this study revealed a surprisingly high frequency of two major mastitis risk factors—bimodality and irregular vacuum fluctuations—occurring more frequently than in conventional milking systems. This study, one of the few comparing different AMS brands and their performance, highlights the crucial role of dynamic testing in evaluating AMS performance under real-world conditions.
Safety of Intraoperative Cell Salvage in Two-Stage Revision of Septic Hip Arthroplasties
Authors: Krüger Lara , Strahl André , Goedecke Eva , Delsmann Maximilian M. , Leonhardt Leon-Gordian , Beil Frank Timo , Hubert Jan
Published: September 2024

(1) Background: The aim of this study was to evaluate the safety of intraoperative cell salvage (ICS) during reimplantation in the two-stage revision of septic hip arthroplasties. (2) Methods: As part of an internal quality control study, blood cultures were taken from the processed ICS blood during reimplantation and examined for possible bacterial load (study group). Due to a high rate of bacterial detection with uncertain clinical significance, consecutive ICS samples were also examined from patients undergoing aseptic revision hip arthroplasty (control group). Microbiological samples, patient and surgical characteristics and the follow-up data were analyzed retrospectively. (3) Results: 9 out of 12 (75%) patients in the study group and 5 out of 8 (63%) patients in the control group had positive ICS blood cultures. There was no significant difference between the groups (p = 0.642). The initial pathogens causing the periprosthetic joint infection (PJI) were not detected, but the bacterial spectrum resembled skin flora, with a high proportion of coagulase-negative staphylococci. No complications due to possible bloodstream-associated infections were observed. In summary, the detected pathogens were interpreted as contamination without clinical significance. (4) Conclusions: ICS in the context of reimplantation was considered a safe and recommendable procedure to optimize patient blood management.
Surgical Site Infection after Primary Open Surgery for Laryngeal Cancer in a Tertiary Hospital in Belgrade, Serbia: A 10-Year Prospective Cohort Study
Authors: Sotirović Jelena , Rančić Nemanja , Pavićević Ljubomir , Baletić Nenad , Dimić Aleksandar , Čukić Ognjen , Perić Aleksandar , Milojević Milanko , Ljubenović Nenad , Milošević Darko , Šuljagić Vesna
Published: September 2024

Background: Surgical site infection (SSI) in laryngeal cancer (LC) patients significantly increases morbidity and may postpone adjuvant therapy. Additionally, SSI can prolong hospitalization, thus representing a burden for the healthcare system. Most of the published studies refer to SSI after salvage laryngectomy. Methods: The present prospective cohort study aimed to clarify the incidence and factors associated with SSI in patients after primary open surgery for LC. Through regular hospital surveillance of patients who underwent primary partial or total laryngectomy, we gathered 24 putative factors and identified SSI from 2013 to 2022. Patients with SSI were compared with patients without SSI. Results: SSI was observed in 21 (6.6%) of 319 patients. ULRA showed that the occurrence of SSI was significantly associated with the American Society of Anesthesiologists (ASA) score, other postoperative healthcare-associated (HAI) infection, T classification, N classification, advanced clinical stage (III–IV), length of stay (LOS), duration of drainage, and the National Healthcare Safety Network (NHSN) risk index. Multivariate logistic regression analysis identified two independent factors associated with SSI occurring in these patients: duration of drainage (RR (relative risk) 1.593; 95% CI 1.159–2.189; p = 0.004) and LOS (RR: 1.074; 95% CI: 1.037–1.112; p < 0.001). Conclusions: Our study provided insight into the burden of SSI in LC patients, highlighting several priority areas and targets for quality improvement.
Evaluation of the Efficacy of Intravenous Push and Intravenous Piggyback Ceftriaxone in Critically Ill Patients
Authors: Sherman Elly R. , Ta Nha Hue , Branan Trisha N. , Patimavirujh Natt , Dickinson Luren Ashton , Bland Christopher M. , Smith Susan E.
Published: September 2024

Background/Objective: Intravenous fluid shortages have led to fluid-sparing measures such as intravenous push (IVP) administration of antibiotics. This study aimed to compare the safety and efficacy of IVP and intravenous piggyback (IVPB) ceftriaxone in critically ill patients. Results: Demographics were similar in IVP (n = 201) and IVPB (n = 200) groups. Sequential Organ Failure Assessment (SOFA) score was higher, and sepsis and septic shock were more common in the IVP group. Treatment failure occurred in 37.8% of IVP and 19.5% of IVPB groups (p < 0.001). Hospital mortality was more common with IVP (21.4% vs. 9.5%, p < 0.001). Hospital LOS was longer with IVP while intensive care unit (ICU) LOS was similar between the groups. IVP ceftriaxone (OR 2.33, 95% CI 1.43–3.79) and the SOFA score (OR 1.18, 95% CI 1.1–1.27) were associated with treatment failure. Methods: A single-center, retrospective cohort study included adults admitted to an ICU from 2016 to 2021 who received empiric ceftriaxone for ≥72 h. The primary outcome was treatment failure, defined as a composite of inpatient mortality or escalation of antibiotics. Secondary outcomes included length of stay (LOS) and mortality. Chi-squared and independent-sample t-tests were used. Treatment failure was evaluated using multivariate logistic regression. Conclusions: Compared to IVPB, IVP ceftriaxone was associated with higher treatment failure in critically ill patients. Both safety and efficacy should be considered before implementing novel antibiotic administration strategies in practice based primarily on convenience.