bluuebunny/crossref_metadata_2025 huggingface dataset:
Salvianolate Ameliorates Osteopenia and Improves Bone Quality in Prednisone-Treated Rheumatoid Arthritis Rats by Regulating RANKL/RANK/OPG Signaling
Authors: Gao Xiang , Wu Qingyun , Zhang Xinle , Tian Jia , Liang Dahong , Min Yalin , Lu Jiaqi , Zhang Xuemei , Cui Liao , Xu Bilian , Liu Yanzhi
Published: September 2021

Rheumatoid arthritis (RA) is closely associated with periarticular osteopenia and leads to a high risk of generalized osteoporosis. Although glucocorticoid (GC) treatment ameliorates joint degradation and manages inflammation in RA, GC application may induce further bone quality deterioration in RA patients. Current treatments for RA lack relevant strategies for the prevention and treatment of osteopenia in RA. In this study, we aimed to investigate whether salvianolate treatment ameliorated osteopenia in prednisone-treated RA rats. Lewis rats with collagen-induced arthritis (CIA) were administered prednisone (PDN) or PDN plus salvianolate (PDN+Sal) treatment for 90 days. The effects of Sal were investigated in PDN-treated CIA rats. To further evaluate the effects of Sal under inflammatory conditions, we investigated the effects of Sal treatment on the TNF-α-induced inflammatory response in MC3T3-E1 osteoblasts. Bone histomorphometry, bone mineral density (BMD), bone biomechanical properties, micro-computed tomography (micro-CT), immunohistochemistry, RT-PCR and western blot analyses were performed to evaluate the effects of Sal. The results demonstrated that RA induced bone loss and bone quality deterioration, with high bone turnover in CIA rats. PDN+Sal treatment significantly increased BMD and trabecular/cortical bone mass, suppressed inflammation, and improved bone biomechanical properties compared to CIA control and PDN treatment. PDN+Sal treatment significantly suppressed bone resorption and the RANKL and RANKL/OPG ratios compared to PDN. PDN+Sal and PDN treatment significantly inhibited TNF-α by 82 and 83%, respectively, and both suppressed inflammation in CIA rats. However, there was no significant difference between PDN+Sal and PDN treatment alone in regard to bone formation parameters or the management of inflammation and arthropathy. Sal significantly increased Osterix, OPN, and Col1a1 while decreasing RANKL, TRAF6, and TRAIL gene in TNF-α-induced MC3T3-E1 osteoblasts. Sal significantly increased Osterix, OPN and RUNX2 while decreasing NF-κB, TRAF6 and IL-1β protein in TNF-α-induced MC3T3-E1 osteoblasts. The results suggested that salvianolate treatment ameliorated osteopenia and improved bone quality in prednisone-treated RA rats, and the potential mechanism may be related to the regulation of the RANKL/RANK/OPG signaling pathway, TRAIL-TRAF6-NFκB signal axis, and downregulation of inflammatory cytokines. Salvianolate could be used as a promising supplemental therapeutic strategy to ameliorate osteopenia and improve bone quality in GC-treated RA patients.
Diverse Roles of Cellular Senescence in Skeletal Muscle Inflammation, Regeneration, and Therapeutics
Authors: Saito Yuki , Chikenji Takako S.
Published: September 2021

Skeletal muscle undergoes vigorous tissue remodeling after injury. However, aging, chronic inflammatory diseases, sarcopenia, and neuromuscular disorders cause muscle loss and degeneration, resulting in muscular dysfunction. Cellular senescence, a state of irreversible cell cycle arrest, acts during normal embryonic development and remodeling after tissue damage; when these processes are complete, the senescent cells are eliminated. However, the accumulation of senescent cells is a hallmark of aging tissues or pathological contexts and may lead to progressive tissue degeneration. The mechanisms responsible for the effects of senescent cells have not been fully elucidated. Here, we review current knowledge about the beneficial and detrimental effects of senescent cells in tissue repair, regeneration, aging, and age-related disease, especially in skeletal muscle. We also discuss how senescence of muscle stem cells and muscle-resident fibro-adipogenic progenitors affects muscle pathologies or regeneration, and consider the possibility that immunosenescence leads to muscle pathogenesis. Finally, we explore senotherapy, the therapeutic targeting of senescence to treat age-related disease, from the standpoint of improving muscle regeneration.
Spin Seebeck Effect in a Hybridized Quantum-Dot/Majorana-Nanowire With Spin Heat Accumulation
Authors: Sun Lian-Liang , Fu Zhen-Guo
Published: September 2021

Properties of spin Seebeck effect (SSE) in a quantum dot (QD) connected to a topological superconductor or semiconductor nanowire with strong spin-orbit interaction are theoretically studied by the noneqilibrium Green’s function method combined with Dyson equation technique. At low temperatures, Majorana zero modes (MZMs) are prepared at the ends of topological superconductor or semiconductor nanowire, and are hybridized to the QD with spin-dependent strength. We consider that the QD is coupled to two leads in the presence of spin heat accumulation (SHA), i.e., spin-dependent temperature in the leads. We find that the thermopower is spin-polarized when the hybridization strength between the QD and one mode of the MZMs depends on electron spin direction, and its spin-polarization can be effectively adjusted by changing the magnitude of SHA. By proper variation of the spin-polarization of the QD-MZM hybridization strength, magnitude of the SHA, dot level, or the direct coupling between the MZMs, 100% spin-polarized or pure thermopower can be generated. Our results may find real usage in high efficiency spintronic devices or detection of the MZMs, which are under current extensive study. The present model is within the reach of current nano-technologies and may by used in high efficiency spin caloritronics devices.
Artificial Intelligence May Predict Early Sepsis After Liver Transplantation
Authors: Kamaleswaran Rishikesan , Sataphaty Sanjaya K. , Mas Valeria R. , Eason James D. , Maluf Daniel G.
Published: September 2021

Background: Sepsis, post-liver transplantation, is a frequent challenge that impacts patient outcomes. We aimed to develop an artificial intelligence method to predict the onset of post-operative sepsis earlier.

Methods: This pilot study aimed to identify “physiomarkers” in continuous minute-by-minute physiologic data streams, such as heart rate, respiratory rate, oxygen saturation (SpO2), and blood pressure, to predict the onset of sepsis. The model was derived from a cohort of 5,748 transplant and non-transplant patients across intensive care units (ICUs) over 36 months, with 92 post-liver transplant patients who developed sepsis.

Results: Using an alert timestamp generated with the Third International Consensus Definition of Sepsis (Sepsis-3) definition as a reference point, we studied up to 24 h of continuous physiologic data prior to the event, totaling to 8.35 million data points. One hundred fifty-five features were generated using signal processing and statistical methods. Feature selection identified 52 highly ranked features, many of which included blood pressures. An eXtreme Gradient Boost (XGB) classifier was then trained on the ranked features by 5-fold cross validation on all patients (n = 5,748). We identified that the average sensitivity, specificity, positive predictive value (PPV), and area under the receiver-operator curve (AUC) of the model after 100 iterations was 0.94 ± 0.02, 0.9 ± 0.02, 0.89 ± 0.01, respectively, and 0.97 ± 0.01 for predicting sepsis 12 h before meeting criteria.

Conclusion: The data suggest that machine learning/deep learning can be applied to continuous streaming data in the transplant ICU to monitor patients and possibly predict sepsis.
Classification of Lung Disease in Children by Using Lung Ultrasound Images and Deep Convolutional Neural Network
Authors: Magrelli Silvia , Valentini Piero , De Rose Cristina , Morello Rosa , Buonsenso Danilo
Published: September 2021

Bronchiolitis is the most common cause of hospitalization of children in the first year of life and pneumonia is the leading cause of infant mortality worldwide. Lung ultrasound technology (LUS) is a novel imaging diagnostic tool for the early detection of respiratory distress and offers several advantages due to its low-cost, relative safety, portability, and easy repeatability. More precise and efficient diagnostic and therapeutic strategies are needed. Deep-learning-based computer-aided diagnosis (CADx) systems, using chest X-ray images, have recently demonstrated their potential as a screening tool for pulmonary disease (such as COVID-19 pneumonia). We present the first computer-aided diagnostic scheme for LUS images of pulmonary diseases in children. In this study, we trained from scratch four state-of-the-art deep-learning models (VGG19, Xception, Inception-v3 and Inception-ResNet-v2) for detecting children with bronchiolitis and pneumonia. In our experiments we used a data set consisting of 5,907 images from 33 healthy infants, 3,286 images from 22 infants with bronchiolitis, and 4,769 images from 7 children suffering from bacterial pneumonia. Using four-fold cross-validation, we implemented one binary classification (healthy vs. bronchiolitis) and one three-class classification (healthy vs. bronchiolitis vs. bacterial pneumonia) out of three classes. Affine transformations were applied for data augmentation. Hyperparameters were optimized for the learning rate, dropout regularization, batch size, and epoch iteration. The Inception-ResNet-v2 model provides the highest classification performance, when compared with the other models used on test sets: for healthy vs. bronchiolitis, it provides 97.75% accuracy, 97.75% sensitivity, and 97% specificity whereas for healthy vs. bronchiolitis vs. bacterial pneumonia, the Inception-v3 model provides the best results with 91.5% accuracy, 91.5% sensitivity, and 95.86% specificity. We performed a gradient-weighted class activation mapping (Grad-CAM) visualization and the results were qualitatively evaluated by a pediatrician expert in LUS imaging: heatmaps highlight areas containing diagnostic-relevant LUS imaging-artifacts, e.g., A-, B-, pleural-lines, and consolidations. These complex patterns are automatically learnt from the data, thus avoiding hand-crafted features usage. By using LUS imaging, the proposed framework might aid in the development of an accessible and rapid decision support-method for diagnosing pulmonary diseases in children using LUS imaging.
B-arrestin-2 Signaling Is Important to Preserve Cardiac Function During Aging
Authors: Capote Andrielle E. , Batra Ashley , Warren Chad M. , Chowdhury Shamim A. K. , Wolska Beata M. , Solaro R. John , Rosas Paola C.
Published: September 2021

Experiments reported here tested the hypothesis that β-arrestin-2 is an important element in the preservation of cardiac function during aging. We tested this hypothesis by aging β-arrestin-2 knock-out (KO) mice, and wild-type equivalent (WT) to 12–16months. We developed the rationale for these experiments on the basis that angiotensin II (ang II) signaling at ang II receptor type 1 (AT1R), which is a G-protein coupled receptor (GPCR) promotes both G-protein signaling as well as β-arrestin-2 signaling. β-arrestin-2 participates in GPCR desensitization, internalization, but also acts as a scaffold for adaptive signal transduction that may occur independently or in parallel to G-protein signaling. We have previously reported that biased ligands acting at the AT1R promote β-arrestin-2 signaling increasing cardiac contractility and reducing maladaptations in a mouse model of dilated cardiomyopathy. Although there is evidence that ang II induces maladaptive senescence in the cardiovascular system, a role for β-arrestin-2 signaling has not been studied in aging. By echocardiography, we found that compared to controls aged KO mice exhibited enlarged left atria and left ventricular diameters as well as depressed contractility parameters with preserved ejection fraction. Aged KO also exhibited depressed relaxation parameters when compared to WT controls at the same age. Moreover, cardiac dysfunction in aged KO mice was correlated with alterations in the phosphorylation of myofilament proteins, such as cardiac myosin binding protein-C, and myosin regulatory light chain. Our evidence provides novel insights into a role for β-arrestin-2 as an important signaling mechanism that preserves cardiac function during aging.
Brief Report: Case Comparison of Therapy With the Histone Deacetylase Inhibitor Vorinostat in a Neonatal Calf Model of Pulmonary Hypertension
Authors: Applegate Tanya J. , Krafsur Greta M. , Boon June A. , Zhang Hui , Li Min , Holt Timothy N. , Ambler S. Kelly , Abrams Benjamin A. , Gustafson Daniel L. , Bartels Karsten , Garry Franklyn B. , Stenmark Kurt R. , Brown R. Dale
Published: September 2021

Pulmonary hypertension (PH) is an incurable condition in humans; driven by pulmonary vascular remodeling partially mediated by epigenetic mechanisms; and leading to right ventricular hypertrophy, failure, and death. We hypothesized that targeting chromatin-modifying histone deacetylases may provide benefit. In this Brief Report we describe case comparison studies using the histone deacetylase inhibitor vorinostat (suberanilohydroxamic acid, 5 mg/kg/day for the first 5 study days) in an established model of severe neonatal bovine PH induced by 14 days of environmental hypoxia. Echocardiographic, hemodynamic, and pharmacokinetic data were obtained in hypoxia-exposed (one each, vorinostat-treated vs. untreated) and normoxic vorinostat-treated control animals (n = 2). Echocardiography detected PH changes by day 4 and severe PH over 14 days of continued hypoxic exposure. RV dysfunction at day 4 was less severe in vorinostat-treated compared to untreated hypoxic calves. Cardioprotective effects were partially maintained following cessation of treatment through the duration of hypoxic exposure, accompanied by hemodynamic evidence suggestive of reduced pulmonary vascular stiffening, and modulated expression of HDAC1 protein and genes involved in RV and pulmonary vascular remodeling and pathological RV hypertrophy. Control calves did not develop PH, nor show adverse cardiac or clinical effects. These results provide novel translation of epigenetic-directed therapy to a large animal severe PH model that recapitulates important features of human disease.
Autophagy Induces Expression of IL-6 in Human Periodontal Ligament Fibroblasts Under Mechanical Load and Overload and Effects Osteoclastogenesis in vitro
Authors: Mayr Alexandra , Marciniak Jana , Eggers Benedikt , Blawat Kim , Wildenhof Jan , Bastos Craveiro Rogerio , Wolf Michael , Deschner James , Jäger Andreas , Beisel-Memmert Svenja
Published: September 2021

Objective:Autophagy is an important cellular adaptation mechanism to mechanical stress. In animal experiments, inhibition of autophagy during orthodontic tooth movement triggered increased expression of inflammation-related genes and decreased bone density. The aim of this study was to investigate how autophagy affects cytokine levels of interleukin 6 (IL-6) in human periodontal ligament (hPDL) fibroblasts under mechanical pressure and the resulting influence on osteoblast communication.

Methods:hPDL fibroblasts were subjected to physiologic mechanical load, constant overload, or rapamycin treatment for 16 to 24 h ± autophagy inhibitor 3-MA. Autophagosomes were quantified by flow cytometry. Gene expression ofil-6as well as IL-6 levels in the supernatant were determined with rtPCR and ELISA. To investigate the influence of mechanically-induced autophagy on cell-cell communication, an osteoblast-culture was subjected to supernatant from stimulated hPDL fibroblasts ± soluble IL-6 receptor (sIL-6R). After 24 h,osteoprotegerin(opg) andreceptor activator of nuclear factorκB ligand(rankl) gene expressions were detected with rtPCR. Gene expression ofa disintegrin and metalloproteinases(adam)10and17in stimulated hPDL fibroblasts was examined via rtPCR.

Results:Autophagy was induced by biomechanical stress in hPDL fibroblasts in a dose-dependent manner. Mechanical load and overload increased IL-6 expression at gene and protein level. Autophagy inhibition further enhanced the effects of mechanical stimulation on IL-6 expression. Mechanical stimulation of hPDL fibroblasts downregulatedadam10andadam17expressions. Inhibition of autophagy had stimulus-intensity depending effects: autophagy inhibition alone or additional application of physiological stress enhancedadam10andadam17expressions, whereas mechanical overload had adverse effects. Osteoblasts showed significantly reducedopgexpression in the presence of supernatant derived of hPDL fibroblasts treated with autophagy inhibitor and sIL-6R.

Conclusion:IL-6 levels were increased in response to pressure in hPDL fibroblasts, which was further enhanced by autophagy inhibition. This caused a decrease inopgexpression in osteoblasts. This may serve as an explanatory model for accelerated tooth movement observed under autophagy inhibition, but may also represent a risk factor for uncontrolled bone loss.
Comparison of Newtonian and Non-newtonian Fluid Models in Blood Flow Simulation in Patients With Intracranial Arterial Stenosis
Authors: Liu Haipeng , Lan Linfang , Abrigo Jill , Ip Hing Lung , Soo Yannie , Zheng Dingchang , Wong Ka Sing , Wang Defeng , Shi Lin , Leung Thomas W. , Leng Xinyi
Published: September 2021

Background

Newtonian fluid model has been commonly applied in simulating cerebral blood flow in intracranial atherosclerotic stenosis (ICAS) cases using computational fluid dynamics (CFD) modeling, while blood is a shear-thinning non-Newtonian fluid. We aimed to investigate the differences of cerebral hemodynamic metrics quantified in CFD models built with Newtonian and non-Newtonian fluid assumptions, in patients with ICAS.

Methods

We built a virtual artery model with an eccentric 75% stenosis and performed static CFD simulation. We also constructed CFD models in three patients with ICAS of different severities in the luminal stenosis. We performed static simulations on these models with Newtonian and two non-Newtonian (Casson and Carreau-Yasuda) fluid models. We also performed transient simulations on another patient-specific model. We measured translesional pressure ratio (PR) and wall shear stress (WSS) values in all CFD models, to reflect the changes in pressure and WSS across a stenotic lesion. In all the simulations, we compared the PR and WSS values in CFD models derived with Newtonian, Casson, and Carreau-Yasuda fluid assumptions.

Results

In all the static and transient simulations, the Newtonian/non-Newtonian difference on PR value was negligible. As to WSS, in static models (virtual and patient-specific), the rheological difference was not obvious in areas with high WSS, but observable in low WSS areas. In the transient model, the rheological difference of WSS areas with low WSS was enhanced, especially during diastolic period.

Conclusion

Newtonian fluid model could be applicable for PR calculation, but caution needs to be taken when using the Newtonian assumption in simulating WSS especially in severe ICAS cases.
Cacao (Theobroma cacao L.) Response to Water Stress: Physiological Characterization and Antioxidant Gene Expression Profiling in Commercial Clones
Authors: Osorio Zambrano Mayra Andreina , Castillo Darwin Alexander , Rodríguez Pérez Loyla , Terán Wilson
Published: September 2021

The increase in events associated with drought constraints plant growth and crop performance. Cacao (Theobroma cacao L.) is sensitive to water deficit stress (DS), which limits productivity. The aim of this research was to characterise the response of seven (CCN51, FEAR5, ICS1, ICS60, ICS95, EET8, and TSH565) commercially important cacao clones to severe and temporal water deficit stress. Ten-month-old cacao trees were submitted to two treatments: well-watered and water-stressed until the leaf water potential (Ψleaf) reached values between −3.0 and −3.5 MPa. The effects of hydric stress on water relations, gas exchange, photochemical activity, membrane integrity and oxidative stress-related gene expression were evaluated. All clones showed decreases in Ψleaf, but TSH565 had a higher capacity to maintain water homeostasis in leaves. An initial response phase consisted of stomatal closure, a general mechanism to limit water loss: as a consequence, the photosynthetic rate dropped by approximately 98% on average. In some clones, the photosynthetic rate reached negative values at the maximum stress level, evidencing photorespiration and was confirmed by increased intracellular CO2. A second and photosynthetically limited phase was characterized by a drop in PSII quantum efficiency, which affected all clones. On average, all clones were able to recover after 4 days of rewatering. Water deficit triggered oxidative stress at the early phase, as evidenced by the upregulation of oxidative stress markers and genes encoding ROS scavenging enzymes. The effects of water deficit stress on energy metabolism were deduced given the upregulation of fermentative enzyme-coding genes. Altogether, our results suggest that the EET8 clone was the highest performing under water deficit while the ICS-60 clone was more susceptible to water stress. Importantly, the activation of the antioxidant system and PSII repair mechanism seem to play key roles in the observed differences in tolerance to water deficit stress among clones.
Overexpression of NtSOS2 From Halophyte Plant N. tangutorum Enhances Tolerance to Salt Stress in Arabidopsis
Authors: Zhu Liming , Li Mengjuan , Huo Junnan , Lian Ziming , Liu Yuxin , Lu Lu , Lu Ye , Hao Zhaodong , Shi Jisen , Cheng Tielong , Chen Jinhui
Published: September 2021

The Salt Overly Sensitive (SOS) signaling pathway is key in responding to salt stress in plants. SOS2, a central factor in this pathway, has been studied in non-halophytes such as Arabidopsis and rice, but has so far not been reported in the halophyte Nitraria tangutorum. In order to better understand how Nitraria tangutorum acquires its tolerance for a high salt environment, here, the NtSOS2 was cloned from Nitraria tangutorum, phylogenetic analyses showed that NtSOS2 is homologous to the SOS2 of Arabidopsis and rice. Gene expression profile analysis showed that NtSOS2 localizes to the cytoplasm and cell membrane and it can be induced by salt stress. Transgenesis experiments showed that exogenous expression of NtSOS2 reduces leaf mortality and improves the germination rate, biomass and root growth of Arabidopsis under salt stress. Also, exogenous expression of NtSOS2 affected the expression of ion transporter-related genes and can rescue the phenotype of sos2-1 under salt stress. All these results revealed that NtSOS2 plays an important role in plant salt stress tolerance. Our findings will be of great significance to further understand the mechanism of salt tolerance and to develop and utilize molecular knowledge gained from halophytes to improve the ecological environment.
Home-School Cultural Value Mismatch: Antecedents and Consequences in a Multi-Ethnic Sample Transitioning to College
Authors: Vasquez-Salgado Yolanda , Greenfield Patricia M. , Guan Shu-Sha Angie
Published: September 2021

Qualitative work has documented home-school cultural value mismatch—a mismatch between collectivistic family obligations and individualistic academic obligations—experienced by Latinx first-generation college students during their first year of study at a 4-year university. This study extends prior research by examining home-school cultural value mismatch among a larger, multi-ethnic sample from Latinx, Asian and European American backgrounds (N = 155) in order to quantify the phenomenon and generalize it across multiple ethnic groups. Antecedents and consequences of different forms of mismatch were assessed in separate models. In modeling antecedents, we found that Latinx background, first-generation college status and low parental income were interconnected. However, among these three variables, it was first-generation college status that was the sole predictor of strong collectivistic motives for attending college; these motives were, in turn, associated with more frequent mismatch between family obligation and academic obligation. In addition, being female directly related to mismatch prevalence, as did living close to home. In modeling consequences of cultural value mismatch, frequent home-school cultural value mismatch predicted mental and physical health distress, which predicted academic problems; such problems were, in turn, related to lower grades. Our findings document the generalizability of this experience for first-generation college students from all ethnic backgrounds, as well as the unique experiences of students who identify as female or live close to home. Our findings also reveal the health and academic costs associated with this mismatch. Implications for research, intervention, and institutional change are discussed and have become increasingly important, given recent societal events that require most students to remain closer to home during distance learning.
Emotional Intelligence and Well-Being of Special Education Teachers in China: The Mediating Role of Work-Engagement
Authors: Fu Wangqian , Wang Chonggao , Tang Wenjun , Lu Sha , Wang Yan
Published: September 2021

The well-being of special education teachers is key to their mental health and also influences the development of their students. This study aimed to investigate the psychological mechanism of the well-being of special education teachers in China, where they are maximum in number. We explored the role of emotional intelligence (EI) and work engagement on the well-being of teachers. A total of 496 Chinese special education teachers participated in the current study. Results suggested that (1) the EI and work engagement were positively associated with the well-being of special education teachers; and that (2) work engagement played a mediating role on EI and well-being of special education teachers. To promote the well-being of special education teachers, suggestions for policymakers and schools are discussed.
Better Writing in Scientific Publications Builds Reader Confidence and Understanding
Authors: Ryba Ren , Doubleday Zoë A. , Dry Matthew J. , Semmler Carolyn , Connell Sean D.
Published: September 2021

Scientific publications are the building blocks of discovery and collaboration, but their impact is limited by the style in which they are traditionally written. Recently, many authors have called for a switch to an engaging, accessible writing style. Here, we experimentally test how readers respond to such a style. We hypothesized that scientific abstracts written in a more accessible style would improve readers’ reported readability and confidence as well as their understanding, assessed using multiple-choice questions on the content. We created a series of scientific abstracts, corresponding to real publications on three scientific topics at four levels of difficulty—varying from the difficult, traditional style to an engaging, accessible style. We gave these abstracts to a team of readers consisting of 170 third-year undergraduate students. Then, we posed questions to measure the readers’ readability, confidence, and understanding with the content. The scientific abstracts written in a more accessible style resulted in higher readability, understanding, and confidence. These findings demonstrate that rethinking the way we communicate our science may empower a more collaborative and diverse industry.
Physical Activity Maintenance: A Critical Narrative Review and Directions for Future Research
Authors: Rhodes Ryan E. , Sui Wuyou
Published: September 2021

A clear rationale can be made for promoting long-term regular physical activity (PA), yet despite some attempts to operationalize “maintenance,” no robust definition has been agreed upon, beyond arbitrary time frames of regular PA. This has likely impaired the advancement of theory and practice. The purpose of this critical narrative review was to first overview the conception of maintenance and co-requisite theoretical constructs in theories used in PA research. Our subsequent aims were to engage in a critical analysis of this literature to propose a working definition of PA maintenance followed by recommendations for future research. Relevant behavioral theories were parsed for references to maintenance or maintenance-specific constructs and constructs most likely associated with maintenance were overviewed from a recent systematic review. Based on this information, we suggest PA maintenance be operationalized as a process marked by a shift in the mechanisms of action determining behavioral performance, that engender greater perceived behavioral enactment efficiency. We suggest that maintenance should not be considered an absolute state of behavioral performance (e.g., a stage), as some constructs that were critical to behavioral performance during initiation will still be critical during PA continuation. Based on this definition, we propose a method of falsifiability hypothesis testing of theoretical constructs that may determine the maintenance process. Finally, the review concludes with suggestions for future research using this operationalization of maintenance including measure development, tests of latency to reach the peak maintenance process, validating constructs critical to determining maintenance, exploration of the contextual and individual moderators of maintenance formation, and the development of an omnibus dynamic model of initiation, continuation, and maintenance in PA behavior change.
Perceptions of Football Analysts Goal-Scoring Opportunity Predictions: A Qualitative Case Study
Authors: Aguado-Méndez Rubén D. , González-Jurado José Antonio , Reina-Gómez Álvaro , Otero-Saborido Fernando Manuel
Published: September 2021

This study aimed to understand the way tactical football analysts perceive the general match analysis issues and to analyze their tactical interpretation of the predictive models of conceded goal-scoring opportunities. Nine tactical analysts responded to the semi-structured interviews that included a general section on the match analysis and a specific one on the results of a study on goal-scoring opportunities conceded by a Spanish La Liga team. Following their transcription, the interviews were codified into categories by the two researchers using Atlas Ti® software. Subsequently, frequency count and co-occurrence analysis were performed based on the encodings. The content analysis reflected that analysts play a crucial role in the analysis of their own team and that of the opponent, the essential skills to exercise as a tactical analyst being “understanding of the game” and “clear observation methodology.” Based on the case study of the conceded goal-scoring opportunities, the major causes and/or solutions attributed by analysts in some of the predictive models were the adaptability of the “style of play” itself according to the “opponent” and “pressure after losing.”
A Study on Chinese EFL Teachers' Work Engagement: The Predictability Power of Emotion Regulation and Teacher Resilience
Authors: Xie Fei
Published: September 2021

Employing a sequential mixed-methods design, the current study examined the role of Chinese EFL teachers' emotion regulation and resilience in predicting their work engagement. To this end, 314 Chinese EFL teachers with various academic degrees and teaching experiences were opted from different schools, institutes, and universities of China. To obtain the quantitative data, Utrecht Work Engagement Scale (UWES), Connor–Davidson Resilience Scale (CD-RISC), and Emotion Regulation Questionnaire (ERQ) were electronically distributed among participants. Performing correlational analyses, a strong association was found between teacher resilience and work engagement. The inspection of the correlations also revealed a moderate correlation between cognitive reappraisal and resilience as well as cognitive reappraisal and work engagement. To probe the predictability power of teacher resilience and emotion regulation (cognitive reappraisal), structural equation modeling (SEM) was performed. The results of the SEM analysis demonstrated that Chinese EFL teachers' work engagement was predicted significantly and favorably by their resilience. Using semi-structured interviews, some qualitative data were also collected to fully understand Chinese EFL teachers' perceptions of work engagement. The thematic analysis (TA) of Chinese EFL teachers' responses to interview questions resulted in two main themes and 14 sub-themes, revealing extrinsic and intrinsic factors contributing to teaching engagement. The findings of TA illuminated that both personal resources and job resources can predict teaching engagement. The pedagogical implications for administrators and teacher trainers are further discussed.
The Effects of Boredom on EFL Learners' Engagement
Authors: Xie Jin
Published: September 2021

This article aims to delve into the role of boredom on students' engagement which has always attracted attention in that it is one of the most common academic feelings felt by students that causes them to feel more or less enthusiastic to engage in class activities, and there is a growing increase in such feelings among adolescents; therefore, its various dimensions should be taken into consideration. First and foremost, a variety of definitions from different points of view have been proposed. Then, with a focus on the distinction between state boredom and trait boredom which is one of the most radical classifications of boredom it has been continued. Following that, the antecedents of boredom are additionally taken into consideration along with the role of boredom in students' engagement that necessitates a few changes in the curriculum of schools. Moreover, some coping strategies on how to overcome boredom have been featured. Finally, in the discussion part, the emphasis of the points, which have been mentioned above, in the learning context for both teachers and students has been discussed, and new suggestions for further studies have been proposed.
Research on Influencing Factors and Dimensions of Health Literacy in Different Age Groups: Before and After the COVID-19 Era in Chongqing, China
Authors: Yang Peiying , Ou Yanran , Yang Hailin , Pei Xuyan , Li Jiarui , Wang Yuxing , Tan Fang , Zhao Xin , Liu Weiwei
Published: September 2021

Background: Understanding the levels of health literacy among different groups is essential for better public health interventions targeting specific subgroups of the population. Additionally, this article explores the prevalence and influencing factors of the health literacy levels of different age groups during the COVID-19 epidemic.

Methods: Multistage stratified cluster random sampling and the Probability Proportion to Size (PPS) method were used to select permanent residents aged 15–69 in Chongqing (54,706) for the questionnaire survey. The survey period is from July 2019 and July 2020. Single-factor analysis and logistic regression models were used to study the relationship between demographics, socioeconomic factors, other independent covariates, and health literacy.

Results: The health literacy levels of residents declined with age, and there were significant differences in health literacy levels between age groups (χ2 = 3332.884, P < 0.05). As far as the factors affecting health literacy level are concerned, high education and high income are the protective factors for health literacy level for residents of all ages. For adolescents (OR = 1.383, 95% CI: 1.217–1.571), young adults (OR = 1.232, 95% CI = 1.117–1.358), and middle-aged people (OR = 1.096, 95% CI = 1.017–1.182), residence in rural areas was a protective factor. In terms of the dimensions of health literacy, in particular, elderly health literacy in 2020 in Scientific Health Concepts, Safety and First Aid, Basic Medical Care decreased significantly compared with 2019.

Conclusions: For adolescents, young adults, middle-aged people, to solve the problem of urban and rural health quality gap, we should not only use the geographical division, but also consider the social population and socio-economic differences. For the elderly, the following four dimensions of health literacy need to be paid more attention than those of other age: Basic Knowledge and Concepts, Scientific Health Concepts, Safety and First Aid, and Basic Medical Care. A lack of knowledge on the prevention and treatment of chronic diseases is the main reason for the recent decline in health literacy. And the health literacy among residents in major public health emergencies is needed.
Poverty, Racism, and the Public Health Crisis in America
Authors: Beech Bettina M. , Ford Chandra , Thorpe Roland J. , Bruce Marino A. , Norris Keith C.
Published: September 2021

The purpose of this article is to discuss poverty as a multidimensional factor influencing health. We will also explicate how racism contributes to and perpetuates the economic and financial inequality that diminishes prospects for population health improvement among marginalized racial and ethnic groups. Poverty is one of the most significant challenges for our society in this millennium. Over 40% of the world lives in poverty. The U.S. has one of the highest rates of poverty in the developed world, despite its collective wealth, and the burden falls disproportionately on communities of color. A common narrative for the relatively high prevalence of poverty among marginalized minority communities is predicated on racist notions of racial inferiority and frequent denial of the structural forms of racism and classism that have contributed to public health crises in the United States and across the globe. Importantly, poverty is much more than just a low-income household. It reflects economic well-being, the ability to negotiate society relative to education of an individual, socioeconomic or health status, as well as social exclusion based on institutional policies, practices, and behaviors. Until structural racism and economic injustice can be resolved, the use of evidence-based prevention and early intervention initiatives to mitigate untoward effects of socioeconomic deprivation in communities of color such as the use of social media/culturally concordant health education, social support, such as social networks, primary intervention strategies, and more will be critical to address the persistent racial/ethnic disparities in chronic diseases.
Prolonged Screen Exposure During COVID-19—The Brain Development and Well-Being Concerns of Our Younger Generation
Authors: Wong Agnes S. K.
Published: September 2021

COVID-19 is a significant public health crisis and it has given a major impact especially in the field of education. The situation has forced educators around the world to shift to an online mode of teaching and children are forced to study online at home. The benefits of online learning are undeniable, but the possible long-term developmental risks of prolonged screen use should not be overlooked. Recent research findings have clearly suggested the negative effects of screen time on the brain development and well-being of our younger generation. Considering the possible long-term developmental risks of prolonged screen use, policy makers should consider appropriate public health policy (e.g., recommendations on screen time) and guideline for the implementations of online learning (e.g., allowing flexibility to suit individual needs). Multidisciplinary collaboration between policy makers, health care professionals, schools, and parents is required to rethink the current situation before it is too late.
Adolescent Running Biomechanics - Implications for Injury Prevention and Rehabilitation
Authors: McSweeney Simon C. , Grävare Silbernagel Karin , Gruber Allison H. , Heiderscheit Bryan C. , Krabak Brian J. , Rauh Mitchell J. , Tenforde Adam S. , Wearing Scott C. , Zech Astrid , Hollander Karsten
Published: September 2021

Global participation in running continues to increase, especially amongst adolescents. Consequently, the number of running-related injuries (RRI) in adolescents is rising. Emerging evidence now suggests that overuse type injuries involving growing bone (e.g., bone stress injuries) and soft tissues (e.g., tendinopathies) predominate in adolescents that participate in running-related sports. Associations between running biomechanics and overuse injuries have been widely studied in adults, however, relatively little research has comparatively targeted running biomechanics in adolescents. Moreover, available literature on injury prevention and rehabilitation for adolescent runners is limited, and there is a tendency to generalize adult literature to adolescent populations despite pertinent considerations regarding growth-related changes unique to these athletes. This perspective article provides commentary and expert opinion surrounding the state of knowledge and future directions for research in adolescent running biomechanics, injury prevention and supplemental training.
Warehouse Storage Management of Wheat and Their Role in Food Security
Authors: Kumar Chandrasen , Ram C. L. , Jha S. N. , Vishwakarma R. K.
Published: September 2021

Silo storage is considered the best option for wheat storage. However, the storage losses in warehouse storage are also equivalent to silo storage of wheat when proper storage management practices are adopted and the Food Corporation of India (FCI) is the best example of a sustainable food storage system with only 0.3% storage losses in 3 years of wheat storage. The wheat procurement, storage, and distribution system of FCI is unique in the world and handles about 85 million tons of food grains annually by procuring from farmers at the guaranteed Minimum Support Price that meets certain quality standards. This article discusses the role of FCI in food security, warehouse storage management practice as a model, and storage loss level of wheat during storage.
Occurrence and Severity of Catheter-Related Bladder Discomfort of General Anesthesia Plus Epidural Anesthesia vs. General Anesthesia in Abdominal Operation With Urinary Catheterization: A Randomized, Controlled Study
Authors: Sun Shunxiang , Wang Cheng , Zhang Jun , Sun Pengfei
Published: September 2021

Background: This randomized, controlled study aimed to investigate the effect of general anesthesia plus epidural anesthesia on catheter-related bladder discomfort (CRBD) in patients who underwent abdominal operation with urinary catheterization.

Methods: A total of 150 patients scheduled for abdominal operation under anesthesia with urinary catheterization were randomized to receive general anesthesia plus epidural anesthesia (N = 74, GA + EA group) or general anesthesia (N = 76, GA group). The occurrence and severity of CRBD, systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were recorded at 0 hour (h), 0.5, 1, and 3 h after tracheal extubation. Besides, postoperative adverse events were assessed.

Results: The occurrence and severity of CRBD at 0, 0.5, 1, and 3 h were all reduced in GA + EA group compared to GA group (all P < 0.05). Meanwhile, subgroup analyses showed that the reduction of occurrence and severity of CRBD in GA + EA group compared to GA group was more obvious in male patients and patients ≥50 years. Besides, SBP at 0, 0.5, 1, and 3 h, as well as DBP at 0, 0.5, and 3 h were all decreased in GA + EA group compared to GA group (all P < 0.05), while HR was increased at 0 h in GA + EA group compared to GA group (P = 0.034). Moreover, the occurrence of pain, severity of pain and occurrence of vomiting were similar between GA + EA group and GA group (all P > 0.05).

Conclusion: General anesthesia plus epidural anesthesia decreases CRBD occurrence and severity with tolerable safety compared with general anesthesia in patients who undergo abdominal operation with urinary catheterization.
Metabolomics Analysis of Splenic CD19+ B Cells in Mice Chronically Infected With Echinococcus granulosus sensu lato Protoscoleces
Authors: Guo Yuxin , Xu Daxiang , Fang Zheng , Xu Shiping , Liu Jiaxi , Xu Zixuan , Zhou Jikai , Bu Zhenzhen , Zhao Yingyi , He Jingmei , Yang Xiaoying , Pan Wei , Shen Yujuan , Sun Fenfen
Published: September 2021

Background: The larval stages of Echinococcus granulosus sensu lato (E. granulosus s.l) infection can alter B cell function and affect host anti-infective immunity, but the underlying mechanism remains unclear. The newly emerging immunometabolism highlights that several metabolites are key factors in determining the fate of immune cells, which provides a new insight for exploring how larval E. granulosus s.l. infection remodels B cell function. This study investigated the metabolomic profiles of B cells in mice infected with E. granulosus s.l. protoscoleces (PSC).

Results:Total CD19+ B cells, purified from the spleen of infected mice, showed significantly increased production of IL-6, TNF-α, and IL-10 after exposure to LPS in vitro. Moreover, the mRNA expression of metabolism related enzymes in B cells was remarkably disordered post infection. In addition, differential metabolites were identified in B cells after infection. There were 340 differential metabolites (83 upregulated and 257 downregulated metabolites) identified in the positive ion model, and 216 differential metabolites (97 upregulated and 119 downregulated metabolites) identified in the negative ion mode. Among these, 64 differential metabolites were annotated and involved in 68 metabolic pathways, including thyroid hormone synthesis, the metabolic processes of glutathione, fructose, mannose, and glycerophospholipid. Furthermore, several differential metabolites such as glutathione, taurine, and inosine were validated to regulate the cytokine production in LPS stimulated B cells.

Conclusion:Infection with the larval E. granulosus s.l. causes metabolic reprogramming in the intrinsic B cells of mice, which provides the first evidence for understanding the role and mechanism of B cells in parasite anti-infective immunity from the viewpoint of immunometabolism.