bluuebunny/crossref_metadata_2025 huggingface dataset:
An Examination of the Technology of Specialised Soft Skill Sets Deployed in Business Sectors from Four Different Regions of the Globe
Authors: Truong Thi Thu Hang , Laura Ronald S. , Shaw Kylie
Published: October 2017

Our central objective in this paper is twofold: first, to examine the scholarly literature on the technological importance of soft skills in Business, and second, to review the major research studies on the views of employers and the industries they serve regarding the specific character and combination sets of key soft skills best suited for business professionals located in four distinct and economically vibrant major business regions of the world. The key findings have been distilled from the literature and analysed to identify patterns of congruence, with the aim of cataloguing reasonably discrete combinatory sets of soft skills ideally suited to the specific business priorities which characterise these four different regions. The concluding section of the paper will consider the extent to which these distinct skill sets can be pedagogically developed in such a way that they become deeply embedded as foundational in the creation of an international business school curriculum, featuring five discrete sets of soft skills, each of which is ideally constructed for one of the four different regions.
IMPACT OF PHARMACIST LED ANTICOAGULATION MONITORING AND PATIENT EDUCATION ON ORAL ANTICOAGULATION THERAPY WITH ACENOCOUMAROL
Authors: Reddy Poojitha Reddy , Mounika Omsri , Chandrika Gaddam , Franklin Aldrich , M Ramesh , Rani N Vanitha , P Thennarasu
Published: October 2017

Objective: Acenocoumarol is anoral anticoagulant with narrow therapeutic index, and the ideal dose of anticoagulation lies between International normalized ratio (INR) values of 2.0 and 3.5. Lack of monitoring and knowledge on acenocoumarol therapy may compromise patient’s safety resulting in under- or over-anticoagulation. A study was conducted to monitor for the safety of anticoagulation therapy, achievement of goal INR levels and to assess the impact of pharmacist led anticoagulation monitoring and patient education on the rational use of acenocoumarol in patients admitted in cardiology wards with thromboembolic disorders.Methods: The study was conducted in 70 patients; data collection was done, prescriptions were analyzed for drug-drug interactions and adverse drug reactions (ADRs). Patients’ knowledge on acenocoumarol therapy was assessed using a self-developed questionnaire at the baseline, then were subjected to a detailed patient education and on an average each patient received three sessions of education. They were again made to answer the same questionnaire on the day of discharge. The mean score of the responses before and after education was compared statistically using Wilcoxon signed rank test and McNemar test.Results: There were 60% patients under anticoagulated with the INR range of >2.0. 123 drug interactions were observed in 58 patients, on an average of 2 interactions per prescription, but no ADRs were observed. 59 patients (84.3%) showed significant overall improvement in knowledge on anticoagulation therapy following education.Conclusion: This study implies the role of clinical pharmacists in achieving better clinical outcomes in patients receiving oral anticoagulation therapy with acenocoumarol.
BETAINE SUPPLEMENTATION FOR VARIOUS CLINICAL DISORDERS
Authors: M Jayalakshmi , V Vanitha
Published: October 2017

Betaine is distributed widely in animals, plants, and microorganisms and rich dietary sources include seafood, especially marine invertebrates. Betaine is N-trimethylated amino acid called as glycine betaine. It is a by-product. Betaine aldehyde is produced when choline dehydrogenase acts on choline, then betaine aldehyde is oxidized to form betaine by aldehyde dehydrogenase. Metabolic derived betaines possess various functions in our body in which they act as methyl donor which helps in liver function, detoxication, and cellular functions. It plays an important role in fat metabolism. Recent research found that betaine can convert homocysteine to cysteine thus they prevent heart disease. Choline is oxidized to betaine in liver and kidney. Intracellular betaine serves as an osmolyte that regulates cell volume and tissue integrity. Betaine not only plays as an osmolyte but also play a major role in the protection of the liver and other tissues. Consequently, it has been proposed that betaine has significant nutrient for prevention of chronic disease. Betaine has been shown to protect internal organs, improve vascular risk factors, and enhance performance. Databases of betaine content in food are being developed for correlation with population health studies. This review focuses on the aspects of wide research field with emphasis on a recent data relevant to various human diseases.
A TREATMENT DILEMMA CONCERNING IMMUNOSUPPRESSANT USE: A CASE REPORT
Authors: Iype Madathil Shinu Mary , Kunoor Akhilesh , Kn Anila
Published: October 2017

Immunosuppressants including corticosteroids (prednisolone >15 mg/day) are responsible for an increased risk of susceptibility to infections (especially pneumonia, tuberculosis [TB] including extra pulmonary TB), an important safety concern when providing immunosuppressive therapy. Anti-TB treatment (ATT) is medicines used to treat tuberculosis, an infectious disease which can affect lungs and other organs. Isoniazid, rifampicin, and pyrazinamide are known to cause ATT induced hepatitis. The prevalence of immunosuppressant induced TB and pyrazinamide induced hepatotoxicity during treatment for active TB ranges to about 0.6%-1% and <1%, respectively. Here, we illustrate a typical case report of a 61-year-old woman who is a known case of interstitial lung disease and developed TB arthritis following therapy with the long-term use of immunosuppressants (prednisolone, azathioprine, leflunomide, and sulfasalazine). ATT regimen was started for TB arthritis which was later modified along with addition of liver protectant due to the development of hepatotoxicity. The causality of both the adverse drug reactions was confirmed to be probable via NARANJO causality assessment scale. This case highlights the incidence of infectious complications like TB which may be expected to be encountered more frequently in the future due to the increasing use of immunosuppressants for the treatment of allergic and inflammatory disorders.
PITYRIASIS ROSEA MIMICKING POLYMORPHIC ERUPTIONS OF PREGNANCY
Authors: Ommurugan Balaji , Priya Amita , Natesh Swaminathan Tambaram
Published: October 2017

ABSTRACTPityriasis Rosea is a self-limiting skin disorder of unknown etiology affecting women more than men. It is very rare in pregnancy and evidence shows conflicting reports on pregnancy outcomes related to Pityriasis Rosea. But however recent evidence says, pregnancy outcomes are not altered, although clinicians must monitor the patient throughout the gestation for adverse outcomes. Hence, we report a case of Pityriasis Rosea infection in a primigravida, in the first trimester and the follow up done in a tertiary care hospital in Southern India.KEYWORDSRASH, FIRST TRIMESTER, ANOMALY SCAN, HUMAN HERPES VIRUS
URINARY MARKERS OF OXIDATIVE DNA DAMAGE IN TYPE 1 DIABETIC CHILDREN: RELATION TO MICROVASCULAR COMPLICATIONS
Authors: El Wakeel Maged A. , Abou-el-asrar Mohamed , El-kassas Ghada Mahmoud , Elabd Mona A , Abu Zeid Dina , Sabry Rania N , Awadallah Eman
Published: October 2017

Objective: Type 1 diabetes mellitus (T1DM) is a widespread metabolic disease, which frequently carries with it a significant impact on human health. Oxidative damage and tissue inflammation have been claimed to be a typical pathogenic component for the progression of diabetic complications. We aim in this study to explore the relation of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) (as a marker of nucleic acid oxidation) to microvascular complications in T1DM.Methods: A case–control study, enrolling 45 T1DM children and an equivalent number of healthy subjects, was performed. Full clinical examination and anthropometric measurement were performed to all subjects. Urinary assessment for 8-oxodG and albumin was done in addition to blood sampling for lipid profile and glycated Hb (HbA1c) assay. Complete ocular examination for assessment of diabetic retinopathy (DR) was also done.Results: Levels of urinary 8-oxodG, serum cholesterol, triglycerides, and low-density lipoprotein in cases were significantly higher than non-diabetics; these levels were likewise higher in uncontrolled T1DM patients in comparison with well-controlled T1DM subjects. Urinary 8-oxodG and HbA1c were significantly higher in diabetic patients with albuminuria and DR compared to patients without complications. Significant positive correlation was found between 8-oxodG with HbA1c (r=0.8, p<0.01), diastolic blood pressure (DBP) (r=0.4, p=0.02), and cholesterol (r=0.4, p=0.05).Conclusion: Urinary 8-oxodG was found to be a reliable marker for assessing oxidative DNA damage in T1DM and can be used in the determination of microvascular complications related to diabetes.
CASE REPORT ON DOCETAXEL-INDUCED INTERSTITIAL LUNG DISEASE
Authors: Antony Remya , Krishna Shiva , James Jini , Kunoor Akhilesh , Vijayan Meenu
Published: October 2017

Docetaxel belongs to taxane family of antineoplastic agents that are indicated for the treatment of locally advanced or metastatic non-small-cell lung cancer. Docetaxel is associated with many adverse effects, and pulmonary reaction has an incidence of 41%. However, interstitial pulmonary disease is found to occur only in <1% of the population as per the post-marketing surveillance reports. Here, we report a case of docetaxel-induced interstitial lung disease (ILD). The patient information and details were collected using the hospital information system. The adverse drug reaction (ADR) of ILD occurred after 5 cycles of chemotherapy with docetaxel at a dose of 60 mg/m2 that is 80 mg IV. The causality of the event in our case was assessed using the Naranjo ADR probability scale which marked a score of 7 (probable). It is necessary that all physicians be aware of the reaction and to closely monitor clinicoradiological and functional status of the patients at regular intervals after administration of docetaxel.
PRIMARY CELL CULTURE OF AEDES ALBOPICTUS MIDGUT CELLS: A PROSPECTIVE MODEL FOR IN VITRO STUDY OF ARBOVIRUSES
Authors: Roy Enakshi , Sinha Moonmoon , Katoch Shailja , Chakraborty Urmita , Das Satadal , Sarkar Debabrata , Nayak Debadatta , Khurana Anil , Manchanda Raj Kumar
Published: October 2017

Objective: Midgut cells play a key role in the propagation of mosquito borne Arboviruses. The existing mosquito cell lines for studying viral pathogenesis are derived either from larvae or from eggs since there is no cell line available from the mosquito midgut. Therefore, to delineate the in situ viral interaction which naturally occurs within the mosquito midgut and represent cellular pathogenesis in human beings, the present work was aimed to develop a primary cell line from the midgut cells of Aedes albopictus.Methods: The midgut cells of A. albopictus were collected, cultured and incubated at 28°C to study the growth after every 24 hrs for 7 days.Result: The primary cell culture showed an increasing growth pattern of columnar cells up to 48 hrs followed by decrease in cell population afterward. However, the number of stem cells increased significantly throughout the study period, and their population outnumbered the columnar cells after 72 hrs. There was no significant change of goblet cells and regenerating cells which were scanty in number throughout the experiment.Conclusion: The present method will help to develop the individual cell lines from mosquito midgut and study the host pathogen interaction in arboviral diseases in future.
MORPHOMETRIC STUDY OF PTERION AND ITS CLINICAL SIGNIFICANCE
Authors: Nayak Gyanaranjan , Bhusan Mohanty Biswa , Ranjan Das Saurjya
Published: October 2017

Objective: Pterion is an area formed by the union of frontal, parietal, temporal, and sphenoid bones. It exhibits variations in the sutural pattern of fusion of constituent bones. Basing on this, the pterion can be classified into four varieties. According to Murphy, these are sphenoparietal, frontotemporal, stellate, and epipteric. The anatomical location of the pterion is necessary for the surgical management of extradural hemorrhage, aneurysm, and brain tumors located in the anterior and middle cranial fossae.The purpose of this study is to find out the prevalence of different varieties of pterion and determine the position of pterion using anatomical landmarks.Methods: The present study included fifty skulls of unknown age and sex. The skulls were studied macroscopically for the anatomical variations of pterion. The distance between the center of the pterion to the midpoint of zygomatic arch and pterion to the frontozygomatic suture was measured using Vernier calipers.Results: The sphenoparietal variety was the most common variety of pterion found in the study followed by the epipteric variety and the stellate variety, respectively. The distance between pterion and back of the frontozygomatic suture was found to be 3.48 ± 0.21 cm on the right side and 3.41 ± 0.16 cm on the left side. The distance between pterion and the midpoint of upper border of the zygomatic arch was found to be 4.01 ± 0.19 cm on the right side and 3.94 ± 0.2 cm on the left side.Conclusion: The findings will be helpful for radiologists and neurosurgeons.
A COMPLETE REVIEW OF MIGRAINE
Authors: Ommurugan Balaji , Priya Amita , Bairy Laxminarayana Kurady
Published: October 2017

Migraine characterized by recurrent headaches present with aura or without aura. Various treatment modalities ranging from 5-hydroxytryptamine 1B/1D agonists, non-steroidal anti-inflammatory drugs to steroids are available for acute treatment of migraine. Prophylaxis for chronic cases is usually with β blockers, calcium channel blockers, and antiepileptics. Even many nutraceutical preparations are helpful in migraine including riboflavin, vitamin b12. This review focuses on the newer agents available for treatment of migraine with some sight into their clinical trials.
AN ALTERNATIVE APPROACH ON BIOREMEDIATION OF HEAVY METALS IN TANNERY EFFLUENTS WASTE USING STREPTOMYCES SP.
Authors: D Thirumurugan , Adamu Karfi Ibrahim , R Vijayakumar , Tg Nithya
Published: October 2017

Objective: The present study is conducted to investigate the abilities of microorganisms to degrade heavy metals in industrial tannery effluent sample.Methods: Tannery effluent sample was collected from effluent treatment plant and analyzed for physicochemical parameters. The potential microbes were isolated and identified by morphological and biochemical characterization. The sample was analyzed before and after to assess the heavy metal reducing the ability of the microorganism and the respective percentage of reduction were studied using X-ray fluorescence spectrometry.Results: The samples were initially found to be highly contaminated with chromium, nickel, and cadmium. Out of three potential isolates, the isolate Streptomyces sp. was found to exhibit a better reduction against chromium (25.7%), cadmium (14.6%), and nickel (23.1%) in 50 ppm at longer incubation period. Comparatively, the reduction abilities of all the three isolates against all the three heavy metals increased with the increase in the incubation period but decreased with the increase in initial metal ion concentration except in the case of Streptomyces sp. against nickel where the reducing ability increased with the increase in metal concentration.Conclusion: Apparently, the present study revealed that Streptomyces sp. had a better remediation potential than the indigenous Pseudomonas sp. and Aspergillus sp. Ultimately, the finding of this research has shown that the Streptomyces sp. can be used as a potent bioremediation agent for treating tannery and industrial effluent in an eco-friendly process.
MICROBICIDAL EVALUATION OF PLANT EXTRACTS - IRON METAL IONS COMPLEX
Authors: Sharma Amarish Kumar , Kaur Jasvir , Sharma Anjana Rana
Published: October 2017

Objective: The aim of this study is to screen and evaluate the antimicrobial or microbicidal effect of plant metabolites, conjugated with essential iron metal ions.Methods: First batch of study includes screening of antimicrobial activity of leaves of mint (Mentha), methi (Trigonella foenum-graecum), and neem (Azadirachta indica) against Bacillus, Aspergillus, and Rhizopus sp., respectively. Inhibition of microbial growth and propagation was investigated using agar well diffusion method (minimum inhibitory concentration evaluation). Antibiotic agent, streptomycin sulfate, and antimycotic agent, and fluconazole was used as a positive control. The polar, non-polar, and aqueous extracts of plants leaves of abovementioned plant species were screened for their antibacterial activity against Bacillus sp. and antifungal activity against Aspergillus and Rhizopus sp. and their mix culture, respectively. Second batch of study includes evaluation of combinatorial effect of plant extracts of mint (Mentha piperita), methi (T. foenum-graecum), and neem (A. indica) complexed with ferric ions (Fe3+, Iron metal ions) in concentrations at acceptable dosage range.Results: The antibacterial and antifungal activity of the plants leaves extracts in different combinations was evaluated. The plant extracts from selected plant of neem, methi, and mint after conjugation with essential iron metal ions and at a concentration of 100 mg/ml show an enhanced microbicidal (2-3 fold) activity compared to only plant extracts used in the experiment.Conclusion: Based on the above investigation, it can be concluded that the plant extracts possessing antimicrobial property when conjugated with essential metal ions (iron metal ions) show enhanced antimicrobial efficacy compared to only plant extracts used. The result from this investigation could be very helpful in the food preservation and packaging industry.
SIZE DEPENDENT APPLICATION OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES AGAINST BACTERIAL SKIN PATHOGENS
Authors: Sunita Patil , Palaniswamy Muthusamy
Published: October 2017

Objective: Bacterial skin infection is one of the most common causes of childhood morbidity in India. Mostly, it is caused by Streptococcus and Staphylococcus infections. However, because of antibiotic resistance in bacterial strains, treatment of skin infections is becoming increasingly difficult. The objective of this research is to study the effect of plant extract concentration on synthesis and morphology of biological silver nanoparticles and investigation of their activity against bacterial skin pathogens.Methods: Biological silver nanoparticles were synthesized using two concentrations (5 and 10 ml) of Aegle marmelos fruit pulp extract. Ultraviolet (UV)-visible spectroscopy, field emission scanning microscopy (FESEM), and high resolution transmission electron microscopy (HRTEM) were used to analyze morphological features of nanoparticles. Antibacterial activity of synthesized silver nanoparticles was studied against the most common skin pathogens Staphylococcus aureus and Streptococcus pyogen, using a well diffusion method.Results: The silver nanoparticles synthesized from 5 ml extract showed UV-absorbance peak at 430 nm with 14-18 nm size, while silver nanoparticles synthesized from 10 ml extract was showed the absorbance at 427 nm with 4-8 nm size. FESEM and HRTEM analysis revealed that both the silver nanoparticles were spherical in shape. Both nanoparticles have shown antibacterial activity among them silver nanoparticles synthesized from 10 ml extract showed better antibacterial activity.Conclusion: This research confirms that plant extract concentration modulate the rate of synthesis, morphology, surface plasmon resonance, and activity of biological silver nanoparticles. Silver nanoparticles synthesized from 10 ml extract can be used efficiently in the treatment of bacterial skin infections.
EFFICACY OF SIMPLE BLEACH DIGESTION OF SPUTUM IN THE DIAGNOSIS OF PULMONARY TUBERCULOSIS BY MICROSCOPIC EXAMINATION
Authors: Yin Leong Grace Corrine Khong , Sharon Leesha , Pandey Pooja , Guldar Nadeem , Bhat Gopalkrishna K
Published: October 2017

Objective: Rapid diagnosis of pulmonary tuberculosis (PTB) depends on microscopic examination of sputum smear for acid-fast bacilli (AFB). The direct microscopy examination lacks sensitivity. It needs at least 10,000 AFB/ml of sputum to observe in the smear. The objective of this study was to determine the efficacy of three different short-term bleach digestions and concentration of sputum in the diagnosis of PTB.Methods: A total of 171 sputum samples collected from patients suspected of having pulmonary TB were used. Direct smear was prepared using each sample and then divided into three parts to which equal volume of 5% bleach was added. The first part was kept at room temperature for 45 minutes with intermittent shaking. The second part was allowed to stand at room temperature for 45 minutes for sedimentation, and the third part was kept at room temperature for 45 minutes and then centrifuged at 3000 g for 15 minutes for deposit formation. Smears were prepared and stained with Ziehl-Neelsen method and observed for AFB using Revised National TB Control Programme Guidelines.Results: Out of 171 sputum samples, 45 (26.32%), 48 (28.07%), 49 (28.65%), and 55 (32.16%) of specimens were positive by direct, bleach digested mixed method, bleach digested sedimented method, and centrifugation method, respectively. The background of the bleach digested sputum smears was clear.Conclusion: Short-term bleach digestion of sputum improves the sensitivity of microscopic examination and the number of AFB observed in the smear. Bleach being a disinfectant kills AFB and makes handling of sputum safer.
ENDOPHYTIC PESTALOTIOPSIS SPECIES FROM ANDAMAN ISLANDS: A POTENTIAL PANCREATIC LIPASE INHIBITOR
Authors: Sarkar Subha Jyoti , Dioundi Divya , Gupta Mahiti
Published: October 2017

Objective: Obesity is the major cause of deaths worldwide. Inhibition of enzymes involved in breakdown of fats can be a good way for obesity management. Pancreatic lipase (PL) causes 50-70% degradation of ingested fats. The aim of this work is to explore the endophytic fungi from untapped biodiversity of Andaman Islands for PL inhibitors.Methods: In the current report, culture broths of 39 endophytic fungi from different curative plants of coastal regions of India have been tested for their potential PL inhibitory activity. The bioactive compound was thus isolated, purified, and analyzed using gas chromatography.Results: It was found that inhibitory concentration of a compound (Rf=0.64) isolated from crude hexane extract of endophytic fungal isolate from Citrus limon was 15.46 μg/ml. Gas chromatogram of the extract showed the presence of caryophyllene which might be responsible for the particular activity. The bioactive fungus was microscopically identified as Pestalotiopsis species.Conclusion: As caryophyllene is component of many oils and is non-toxic, so it can be potential source of safe and effective anti-obesity drug.
BIODEGRADABLE POLYMER: A NOVEL PHARMACEUTICAL CARRIER FOR SUSTAINED RELEASE OF METRONIDAZOLE
Authors: Hariharan Gayathri , Sinha Priyanka
Published: October 2017

Objective: To optimize and evaluate the formulation of metronidazole (MT)-loaded chitosan microspheres and to investigate the efficiency of biodegradable polymer in developing sustained release formulation of MT to prolong the action of drug.Methods: MT microspheres were prepared using emulsion cross-linking method. Polymer-drug compatibility study was done using Fourier transform infrared. Physical characteristics were evaluated by particle size,SEM, flow properties etc. In vitro studies for evaluating drug release for MT-loaded chitosan microspheres were done by dissolution study.Results: Particle size of the formulated microspheres was found to be within the range of 110-130 μm. Flow properties of F1-F7 such as angle of repose, bulk density, and tapped density were found to be within limits. Drug entrapment efficiency was found to be better for all the formulations within the range of 74.82-84.32% w/w. Drug loading capacity was found to be in the range of 56-83.2% w/v. In vitro drug release was found to be in the range of 81.32-96.23% w/v.Conclusion: In spite of all the above results, we conclude that F5 formulation was optimized depending on the data obtained from the drug loading capacity and percentage drug release studies. F5 formulation is formulated with drug-polymer ratio 1:2 with 1% of di octyl sodium sulfo succinate and 8 ml of glutaraldehyde as a cross-linking agent.
EVALUATION OF FETAL WEIGHT SONOGRAPHICALLY USING AREA OF WHARTON’S JELLY AND MORPHOLOGY OF UMBILICAL CORD
Authors: Behera Shashi Shankar , Behera Shashi Shankar , Chinara Prafulla Kumar
Published: October 2017

Objective: To establish a sonographic relationship between Area of Wharton’s Jelly (AWJ) and umbilical cord morphometry with the birth weight of the fetus in low-risk pregnancies from 13 to 40 weeks.Methods: A total of 800 singleton pregnant females were subjected for routine sonographic evaluation. The umbilical cord length, diameter, and AWJ were determined. The gestational age and fetal weight were determined using usual fetal parameters. Umbilical cord morphometry along with Area of Wharton Jelly can be utilized as other parameters to increase the accuracy of fetal weight.Results: In our study, the umbilical cord diameter at birth showed statistically significant positive correlation with birth weight (R=0.167; p<0.001). Umbilical cord length, diameter, and Area of Wharton Jelly showed statistically significant positive correlation with birth weight (p<0.001).Conclusion: Using statistical analysis, a positive correlation was established between estimated fetal weight and fetal age with umbilical cord morphometry and AWJ.
MOLECULAR IDENTIFICATION OF PROBIOTIC YEAST STRAINS AND THEIR CHARACTERIZATION
Authors: Lakshmi Ragavan Mangala , Das Nilanjana
Published: October 2017

Objective The objective of the present study was to identify the potential yeast isolates at themolecular level and evaluate their probiotic characteristics.MethodsMolecular characterization was done for 5 potential probiotic yeast strains. In vitro assays have been conducted to evaluate the probiotic properties such as NaCl tolerance, autoaggregation and co-aggregation. Haemolyticactivity, urease activity and cytotoxicity tests were carried out for safety assay during the characterization of yeast strains.ResultsIn this study, the yeast strains viz. LM,MR,GOI,GII2and WI were identified at molecular level and named as Yarrowialipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera VIT-MN04, Brettanomyces custersianus VIT-MN05 respectively. Maximum autoaggregation and coaggregationwere noted in case of Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligeraVIT-MN04, and Brettanomyces custersianus VIT-MN05. In vitro toxicity assay was performed and all the yeast strains showednon-toxic nature.ConclusionFive yeast strains have been studied for their probiotic characteristics and identified at molecular level. Out offive yeast strains, three strains showed maximum adhesion ability,which is a prerequisite for colonization and protection of gastrointestinal tract. All the yeast strains are validated as a safe bioresources because of their non - hemolytic activities and non-production of urease. It can be concluded that the identified yeast strainscan serve as promising probiotics in various fields offood industry.
ALBUMIN AND ITS ROLE IN UROLITHIASIS
Authors: Raizada Devesh , Kumar Pragya , Singh Tanya , Pruthi Trisha , Riyadarshini Riyadarshini
Published: October 2017

Urolithiasis is a multifactorial disease with an incidence rate of more than one million cases reported annually in India. Various forms of the calculus have been reported to have 90-95% inorganic and 5% organic matter. Out of the major proteins that comprise this organic component of the matrix, albumin, and uromodulin are found to be the most abundant. Albumin is also the most abundant protein in the human blood serum where it plays the role of a transporter of hormones, fatty acids, and other compounds. The increased concentrations of albumin may significantly affect a patient’s susceptibility to kidney stone formation. The study of the role of albumin in urolithiasis could give us useful insights on its potential role in this disease and may add to the therapeutic repertoire of albumin.
THE RELIABILITY OF THE BOLTON RATIO WHEN APPLIED TO THE INDONESIAN POPULATION
Authors: Kusnoto Joko
Published: October 2017

Objective: The objective of this study is to determine whether Bolton ratio can be applied clinically to the Indonesian population and to determine a more suitable Bolton ratio for the Indonesian population.Methods: This study was conducted on 120 readily available study models of treated cases comprising 37 males and 83 females. Two investigators separately measured the mesiodistal crown width of maxillary and mandibular tooth on each study model using sliding calipers. According to Bolton’s method, the overall and anterior ratios from each sample were calculated and the mean was generated. Using Student’s t-test with a 95% confidence interval, the investigators compared whether there is a significant difference between the ratio from Bolton’s samples and the ratio from the Indonesian samples.Results: The result of this study showed that, for Indonesian samples, the overall ratio is 89.7±2.05, while the anterior ratio is 76.4±2.76. Student’s t-test showed that there is a statistically significant difference (p<0.05) between the results of this study and that of Bolton’s study for both the anterior and overall ratios.Conclusion: It can be concluded that there is a difference between Indonesian population and Caucasian population in tooth size and Bolton ratio value. Therefore, original Bolton ratio value cannot be used as an accurate diagnostic tool for Indonesian population.
ANTIMICROBIAL ACTIVITY OF ANISOCHILUS CARNOSUS (L.F.) WALL AGAINST THE HUMAN GASTRIC PATHOGEN HELICOBACTER PYLORI
Authors: Shetty Vignesh , Lobo Richard , Kumar Nimmy , Lingadakai Ramachandra , C Pai Ganesh , Balaraju Girish , Ballal Mamatha
Published: October 2017

Objective: The prevalence of Helicobacter pylori in India is high, and majority leads to severe gastrointestinal infections. Existing treatment regimens for H. pylori infections have increased failure rates and adverse side effects that desire the search for an effective substitute therapy. Anisochilus carnosus (L.f.) wall (Lamiaceae), a herb which grows once in a year at high elevation is used widely in traditional treatment for the complaints of gastric ulcer and skin diseases. The present study was performed to assess the antibacterial activity of A. carnosus (L.f.) wall, against clinical isolates of H. pylori in vitro.Methods: A. carnosus leaves were collected-dried and extracted with water and ethanol by cold maceration with ethanol by soxhlet method. The minimum inhibitory concentration (MIC) of extracts was made and tested against 32 clinical and 1 reference strains of H. pylori. Results: A. carnosus (L.f.) wall inhibited the growth of most of the clinical H. pylori strains. The MIC of A. carnosus (L.f.) wall extracted by cold maceration (aqueous and ethanol) and Soxhlet apparatus (ethanol) ranged from 500 to 62.5 μg/ml, and the majority of the clinical H. pylori strains were inhibited at the MIC of 500 μg/ml of aqueous, ethanol, and Soxhlet ethanol extraction were 63.63%, 43.75%, and 71.87%, respectively.Conclusion: A. carnosus (L.f.) wall is an efficient inhibitor of H. pylori growth in vitro. A. carnosus (L.f.) wall revealed enormous therapeutic potential to H. pylori infection as it was extremely active in the suppression of H. pylori. Hence, it can be taken as a potential agent against several H. pylori linked gastric pathogenic progressions.
MICROSURGICAL CLIPPING IN THE RUPTURED AN ANEURYSM OF ANTERIOR COMMUNICATING ARTERY: A CASE REPORT
Authors: Niryana Wayan , Pagehgiri Aslesa Wangpathi , Widyadharma Putu Eka
Published: October 2017

Objective: Ruptured saccular aneurysms are a common and serious medical problem. The prevalence of aneurysms is low during the first two decades of life and increases steadily after the third decade. Anterior communicating artery (AComA) aneurysms are regarded as the most complex of all intracranial aneurysms.Methods: Here, we report a case of an aneurysm of AComA patient with the development of microsurgical technique and intraoperative monitoring technique.Results: Permanent clipping was successfully performed in the neck of an aneurysm of AComA.Conclusion: Microsurgical clipping still remains a definitive treatment of ruptured cerebral aneurysms.
THE INVESTIGATION OF CONDITIONS OF API FROM GROUP OF CALCIUM CHANNEL BLOCKERS EXTRACTION BY ORGANIC SOLVENTS BY USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AS ASSAY METHOD
Authors: Polyauk Olgya , Logoyda Liliya
Published: October 2017

Objective: The objective of this research was to select the optimal conditions for extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions.Methods: The chromatographic analysis of amlodipine, nifedipine, and verapamil performed on liquid chromatography Agilent 1200.Results: The quantitative determination of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by high-performance liquid chromatography - method has been conducted. As a result of studies, we have found that the optimal extragent is chloroform, which is extracted at pH 7-82.1% of amlodipine, pH 5-76.2% of nifedipine, and pH 8-95.1% verapamil hydrochloride. API from calcium channel blockers (amlodipine, nifedipine, and verapamil) least extracted with hexane at pH 2.0, so these conditions may be cleaned extract from coextractives impurities.Conclusion: The results obtained in this research work clearly indicated that the extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions has been conducted.
ESTIMATION OF CURCUMINOIDS IN CURCUMA KARNATAKENSIS (WHITE TURMERIC) - AN ENDEMIC TAXON
Authors: Dh Tejavathi , Bs Sujatha , R Kannan
Published: October 2017

Objective: To estimate the contents of curcuminoids in two samples of Curcuma karnatakensis collected from different localities.Methods: Quantification of curcuminoids was estimated by ultra-high performance liquid chromatography-mass spectrometry/selected reaction monitoring (UHPLC-MS/SRM) method in two samples of C. karnatakensis for the first time. Fine powder of rhizomatous rootstocks of two plant samples collected from different habitats were served as Samples A and B. The MS used for the metabolite analysis is a Vantage TSQ triple stage quadrupole MS equipped with heated electrospray ionization. The MS is coupled with an Agilent 1290 infinity UHPLC system. A stock solution of curcuminoid standard was prepared by dissolving 5 mg of standard in 1 mL of methanol. Seven different concentrations of standard (0.15-10 ng on column) were injected for the UHPLC-MS/SRM analysis. Separations were performed using a C-18 column with a flow rate of 0.2 mL/minute.Results: Contents of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found to be varied in two samples and lowest than any other species of Curcuma studied. Variation in the contents may be due to their different habitats in which they are growing.Conclusion: The present attempt of analyzing the contents of curcuminoids in this endemic taxon for the first time will provide the basis for further pharmacological analysis to authenticate the efficacy of these active principles as the curcuminoids are known for varied pharmacological activities.
THE EVALUATION OF ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF THE ESSENTIAL OIL EXTRACTED FROM THE AERIAL PARTS OF SOUTHERNWOOD HERB (ARTEMISIA ABROTANUM L.) THAT RECENTLY GROWN IN IRAQ
Authors: Almahdawy Sarah S. , Said Ali Muaffaq , Abbas Ibrahim S , Dawood Ashour H
Published: October 2017

Objective: This research is to study the assessment of the antimicrobial and cytotoxic activity of the essential oil extracted from the aerial parts of Artemisia abrotanum L. that recently grown in Iraq.Methods: The essential oil of A. abrotanum was extracted by hydrodistillation using Clevenger apparatus. This essential oil was tested for antimicrobial activity of five different pathogenic microorganisms (Gram-positive [Staphylococcus aureus and Bacillus subtilis] and Gram-negative [Salmonella typhi and Escherichia coli] bacterial strains) and fungi: Candida albicans using diffusion well agar method. Furthermore, this essential oil was tested for cytotoxic activity using rhabdomyosarcoma cell line, and the growth or inhibition of cancer cells was measured by MTT method.Results: The obtained results show that the antibacterial activity for A. abrotanum against S. aureus was at concentrations 40, 25, and15 μl with minimum inhibitory concentrations of 20 mm, while it showed antibacterial activity against E. coli for four different concentrations of 40, 25, 15, and10 μl with inhibition zone of 16, 12, 14, and 10 mm, respectively, and it showed antifungal activity against C. albicans at four concentrations 40, 25, 15, and10 μl with inhibition zone of 18, 24, 26, and 30 mm, respectively. The cytotoxic activity of the extracted essential oil was showed that the three concentrations of the extract (25, 50, and 100 μg/ml) were all lower significantly as compared to dimethyl sulfoxide group. A significant difference was seen for group 25 with both groups 50 and 100, but no significant difference was seen between the two later. Finally, the antimicrobial and anticancer activity of this plant could be due to its essential oil constituents: Borneol, cymene, camphor, terpineol, eucalyptol, and aromadendrene.Conclusion: The essential oil of A. abrotanum L. has a potent antimicrobial and anticancer effect against the tested microbial organisms and the cancer cells.
Cambios de la fracción hidrosoluble de huevo de gallinas alimentadas con harina de camarón almacenado a diferentes tiempos y temperaturas
Authors: Carranco Jáuregui María Elena , Carrillo-Domínguez Silvia , Fuente-Martínez Benjamín , Ávila-González Ernesto , Solano María de Lourdes
Published: October 2017

El objetivo de este estudio fue determinar el efecto de las condiciones de almacenamiento, sobre la fracción hidrosoluble del huevo de gallinas alimentadas con una dieta convencional y una dieta con 20 % de harina de cabezas de camarón (HCC). Noventa (90) gallinas Isa-Brown de 32 semanas de edad se distribuyeron en dos tratamientos: Testigo y HCC. Durante cuatro semanas de ensayo se evaluaron las variables productivas. Los huevos recolectados (n= 250) para este estudio, se almacenaron por 15 y 30 días, a 20° y 4 °C, tomando como referencia huevos recién puestos (sin almacenar). Los parámetros estudiados fueron peso de huevo, Unidades Haugh, pH, proteína, aminoácidos indispensables y vitaminas hidrosolubles. Los resultados se analizaron utilizando un diseño factorial 2x2x3 y las diferencias entre medias se compararon mediante la prueba de Tukey. Los resultados obtenidos mostraron que los índices de calidad de la fracción hidrosoluble de huevo se vieron afectadas significativamente (P<0.05) por el tiempo y la temperatura de almacenamiento, y diferencias (P<0.05) solo en proteína por el efecto de la inclusión de HCC. A temperatura ambiente (20 °C) y mayor tiempo de almacenamiento (30 días), provocó una disminución en la calidad interna del huevo de acuerdo a los resultados de este estudio. Por lo tanto, se concluye que tanto la temperatura como el tiempo son uno de los principales factores que llegan a influir en la calidad de la fracción hidrosoluble del huevo durante el almacenamiento.
KEPADATAN VEKTOR DEMAM BERDARAH DENGUE DI PERUMNAS SITEBA PADANG TAHUN 2008
Authors: Irawati Nuzulia
Published: October 2017

AbstrakDemam Berdarah Dengue (DBD) masih merupakan masalah kesehatan masyarakat terpenting di Indonesia. Penyakit ini disebabkan oleh virus dengue, yang disebarkan melalui gigitan nyamuk Aedes aegypti sebagai vektor utama. Penyakit ini menyebar bersamaan dengan peningkatan mobilitas penduduk, kepadatan penduduk dan transportasi. Penyelidikan telah dilakukan dalam rangka memperoleh jawaban tentang kepadatan vektor DBD di Perumnas Siteba menggunakan metode penelitian Survey Deskriptif (Cross Sectional). Dalam hal ini, 100 buah rumah digunakan sebagai sampel sedangkan teknik pengambilan sample adalah Multi Stage Random Sampling. Hasil yang didapatkan 33% rumah dan 20.7% kontainer positif mengandung larva aedes dan 100% berupa Aedes aegypti. Kesimpulan dari penelitian ini adalah kepadatan vektor DBD di Perumnas Siteba Padang cukup tinggi dengan ratio 3: 1, dengan vektornya Aesdes aegypti. Hal ini dapat dilihat dari salah satu rumah yang positif larva Aesdes aegypti.Kata lunci : Aedes aegypti, Dengue Haemorrhagic fever (DHF)AbstractDengue Hemorrhagic fever (DHF) is still an critical community health problem in Indonesia. This disease is caused by Dengue Virus, which is spread through Aedes aegypti’s bite as the main vector. This disease spread together along with the increasing of people’s mobiliy, the population density and the transportation. The research has been done in order to get the description of the density 0f DHF vector in Perumnas Siteba by using Descriptive Survey (Cross Sectional) research method. In this case, one hundred houses are used as the sample, while the technique of taking the sample is in Multistage Random Sampling. The result 0f above 33% house and 20.7% the containera, positively with Aedes larva and almost 100% vector’s species are Aedes aegypti. In this respect, it can be concluded that the density of DHF Vector in Perumnas Siteba Padang is ther high, in ratio 3:1. It can be seen from one of three houses are positive with Aedes aegypti larvae.Key word : Aedes aegypti, Dengue Haemorrhagic fever (DHF)ARTIKEL PENELITIAN
LIMFOMA MALIGNA PADA MATA YANG DIRAWAT DI RUMAH SAKIT Dr. M. DJAMIL PADANG TAHUN 2003-2010
Authors: Putri Linda Wira , Rahman Ardizal
Published: October 2017

AbstrakMelaporkan kasus Limpoma pada Mata yang dirawat di RS. Dr. M. Djamil Padang.Studi retrospektif dari 17 rekam medik yang didiagnosa sebagai limfoma pada mata di Bagian Mata RSUP dr. M. Djamil antara 2003 – 2010.Terdapat 17 kasus limfoma pada mata di RSUP Dr. M. Djamil antara 2003 – 2010. Pasien laki-laki sebanyak 15 orang dan pasien perempuan 2 orang, dengan usia antara 4 bulan – 71 tahun. Keluhan utama paling sering (82,35%) adalah proptosis pada mata. Semua pasien diperiksa dengan CT scan dan pemeriksaan histopatologis. Tipe limfositik yang paling banyak ditemukan secara histopatologis (64,70%). Semua pasien dianjurkan untuk kemoterapi, tetapi hanya 10 pasien yang menjalani kemoterapi.Proptosis pada mata merupakan tanda yang paling sering dari limfoma maligna pada pasien yang berobat di RSUP Dr. M. Djamil. Tipe limfositik adalah tipe yang paling sering. Tidak semua pasien menjalani kemoterapi karena adanya masalah ekonomi.Kata kunci: Limfoma maligna, limfoma pada mata, proptosisAbstractReporting cases of hospitalized ocular lymphoma at Dr. M. Djamil Hospital from 2003 – 2010.Retrospective study of the medical records of 17 Ocular Lympomas diagnosed patients at the Ophthalmology Department of Dr. M. Djamil Hospital, between 2003 – 2010.There were 17 cases of Ocular Lymphoma at Dr. M. Djamil Hospital between 2003 – 2010. They are 15 male and 2 female patients, in range of age between 4 months to 71 years old. Most of them (82.35%) have chief complaint proptosed of the eye since months to years. All patients were examined using CT imaging and histopatologically. Lymphosityc type was the most type of hystopathological characterictic (64.70%) All patient were suggested to chemotherapy, but only 10 patients received chemotherapy.LAPORAN KASUS51Proptosed of the eye was the most commont sign of malignant lymphoma patient visited at Dr. M. Djamil Hospital. Lymphosityc type was the most common type. Not all patients could receive chemotherapy for they have economic problems.Key word : Malignant lymphoma,ocular lymphoma, proptosis
DIAGNOSIS DAN TATALAKSANA INFEKSI HIV PADA NEONATUS
Authors: Darmadi Darmadi , Ruslie Riska Habriel
Published: October 2017

AbstrakPada tahun 2009, 1,4 juta wanita hamil di negara berpendapatan menengah dan rendah terdiagnosis HIV. Identifikasi awal infeksi HIV, pemberian terapi antiretrovirus, dan supresi virus ke level yang tidak terdeteksi sudah terbukti memperpanjang usia hidup dan mencegah morbiditas pada pasien dewasa. Secara teori prinsip supresi virus pada bayi tidak berbeda dengan dewasa. Namun data mengenai manajemen infeksi HIV pada anak khususnya neonatus masih terbatas. Sehingga disusun literatur yang membahas diagnosis dan manajemen infeksi HIV pada neonatus.Kata kunci. HIV, HIV di neonatus, transmisi vertikal, antiretrovirusAbstractIn 2009, 1.4 million pregnant women in low and middle-income countries diagnosed HIV. The early identification of HIV infection, the administration of antiretroviral therapy, and the suppression of viral loads to undetectable levels all have been shown to prolong life and prevent morbidity in adults. In theory the principle of viral load suppression would apply to infants as well as adults. However data about the management of HIV infection in children especially in newborns is limited. So in this review examines the diagnosis and management of HIV infection im newborns.Key word : HIV, neonatal HIV, vertical transmission, antiretroviral