bluuebunny/crossref_metadata_2025 huggingface dataset:
Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris
Authors: Geethanjali Karli , Rathnagiri Polavarapu , Kalarani Varada
Published: April 2023

Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure. Except for a few lab-based assays, to date, there are no point-of-care diagnostics developed to screen for the presence of infectious agents in cattle. In this study, we have identified cysteine protease 8 as a suitable antigenic protein for developing sero-diagnostics. A 960 bp Tf CP8 gene was cloned into methylotrophic Pichia pastoris X-33 by homologous recombination using a pPICZαA vector for recombinant protein expression. The traditional fed-batch method of induction with methanol resulted in inconsistent expression in 48h incubation, hence a novel single batch culture with 1% methanol induction for 24h was standardised and obtained optimal recovery of approximately 36 KDa recombinant protein secreted into media. To the best of our knowledge, this is the first report of cloning and expression of genes from Trichomonas foetus. This CP8 protein could be further optimised for developing lateral flow assays and ELISA as point-of-care tools.
Extraction and characterization of essential oil of nutmeg (Myristica fragrans) fruit pericarp and utilization as a surface coating material to improve the shelf life of paneer
Authors: Vyshak V. L. , Rahila M. P. , Faisal I. , Lukose Smitha J. , Divya M. P. , Sudhakaran Aparna Sudhakaran , Rajakumar S. N.
Published: April 2023

Paneer is a traditional dairy product of India and is similar to unripened soft cheese. It is used as a raw material for the preparation of a variety of culinary dishes and snacks. Paneer is marble white in appearance, with a firm, cohesive and spongy body and a sweetish-acidic-nutty flavor. But it is highly perishable with a limited shelf- life like other indigenous dairy products. Its shelf life was reported to be only six days under refrigeration, though its freshness is lost within three days. The spoilage of paneer occurs mainly due to the growth of microorganisms, which bring about various physico-chemical changes. In the present study, attempts were made to increase the shelf-life of paneer at refrigerated storage by the application ofnutmeg pericarp essential oil surface coating. The essential oil was prepared from fresh nutmeg fruit flesh by steam distillation method. The physico-chemical and bio functional properties of essential oil were determined. The specific gravity and refractive index of the essential oil were found to be 0.903 and 1.477 respectively. The acid value and total polyphenolic value of essential oil were 3.36 and 4.04 mg GAE/g respectively. The essential oil was also found to have good antimicrobial and antioxidant activity. The shelf life of samples coated with essential oil packaged in LDPE pouches had more shelf life than the control paneer, as the coated paneer kept well for 9 days while the control paneer spoiled after the 6th day of refrigerated storage. The paneer with essential oil coating obtained satisfactory sensory scores.
Molecular characterisation of antimicrobial resistance in coagulase negative staphylococci isolated from bovine subclinical mastitis
Authors: Anaina S. , Vijayakumar K. , Davis K. Justin , Rathish R. L. , Mani B. K.
Published: April 2023

Coagulase negative staphylococci (CNS) are emerging as the most prevalent causative agent of bovine mastitis. They are resistant to many commonly used antibiotics due to the presence of antimicrobial resistance (AMR) genes. A study was conducted to evaluate the AMR profiling of CNS isolated from bovine subclinical mastitis.Coagulase negative staphylococci were isolated from 49 (44.95 per cent) of the subclinical mastitis samples. Disc diffusion assay revealed that highest resistance was shown against gentamicin (42.85 per cent) followed by methicillin (32.6 per cent), ceftriaxone – tazobactam (24.48 per cent), enrofloxacin (20.4 per cent), tetracycline (16.32 per cent) and least resistance to cotrimoxazole (4 per cent). Genotypic characterisation of AMR genes such as mecA, aacA-aphD and norA by PCR was done for determining resistance to methicillin, gentamicin and fluroquinolone resistance. The CNS carried aacA-aphD, norA and mecA in 44.89 per cent, 32.65 per cent and 14.28 per cent, respectively. Comparison of phenotypic and genotypic characterisation of AMR in CNS was carried out by McNemar test and it was found that there was significant difference between the presence of mecA gene and methicillin resistance. There was no significant difference noticed forcharacterisation of phenotypic and genotypic AMR of CNS for gentamicin and fluroquinolone resistance.
Evaluation of the effect of soy protein isolate based milk replacer feeding on digestibility of nutrients and haematobiochemical parameters in cross bred calves
Authors: Abhijith M. , Purushothaman Sajith , Ally K. , Shyama K. , Gleeja V. L.
Published: April 2023

A study was conducted in crossbred calves for a period of 90 days to evaluate the effect of soy protein isolate based milk replacer feeding on digestibility of nutrients and haematobiochemical parameters.Twelve,four-day-old crossbred calveswere selected from University Livestock Farm and Fodder Research and Development Scheme (ULF & FRDS), Mannuthy and were allotted to one of the two dietary treatments. The dietary treatments were T1 (Control) fed with whole milk and T2- fed with soy protein isolate based milk replacer (CP-22 per cent) and milk in the ratio 3:1.To all the experimental animals, calf starter (CP-23 per cent) and adlibitum green grass were offered and were fed according to ICAR (2013). Daily record of quantity of feed (calf starter and milk replacer) andgrass given and balance if any were recorded throughout the experimental period. A digestibility trial for five days duration was carried out towards the end of feeding trial by total collection method. The results revealed similar apparent digestibility of nutrients and haematobiochemical parameters for whole milk and milk replacer fed group.
Detection of humoral and cell mediated immune responses among breeder ducks vaccinated against riemerellosis
Authors: Mathews Lina Kunjamma , Priya P. M. , Mini M. , Sankar Surya , Chacko Binoj , Raju Greeshma , A. Athira
Published: April 2023

Riemerellosis is an infectious disease that primarily affects young ducklings, although it can infrequently be manifested in a chronic localised form in older ducks. Though an inactivated vaccine has been found to be most effective in preventing the disease in ducklings, its potency in adult breeder ducks has not been assessed yet. To investigate the same, 200 Kuttanad breeder ducks maintained in the University Poultry and Duck Farm (UPDF), Mannuthy were grouped into two, of 100 birds each, T1 being the control group and T2, the treatment (vaccinated) group. The oil-adjuvanted inactivated vaccine, prepared as per the previously standardised protocol, was administered to the T2 group in two doses, one week apart. Blood samples in serum vials, 20 each from T1 and T2, were collected on days 0, 14, 28, 56 and 90 post-immunisation (PI) whereas blood samples in heparin vials, eight each from both the groups, were collected on days 0, 14, and 28 PI. Humoral immunity (HI) and cell mediated immunity (CMI) in the adult ducks were assessed by enzyme-linked immunosorbent assay (ELISA) and lymphocyte proliferation assay (LPA), respectively. The two assays revealed that the inactivated vaccine elicited a good immune response in the adult ducks, with HI noticed until 90th day PI and an increasing CMI that peaked on 28th day PI.
Seroprevalence and risk factors of human cystic echinococcosis in Central and North Kashmir, India
Authors: Andrabi Abas , Tak Hidayatullah , Fomda Wasim Muzamil Bashir , Para Bilal Ahmad , Shah Ishteyaq Majeed , Majeed Ishfaq
Published: April 2023

Cystic echinococcosis (CE) is a dreadful zoonotic neglected disease that affects public health globally. The infection is highly endemic in northern India (Kashmir valley). The present survey based study was done to know the seroprevalence of cystic echinococcosis infection among residents of north and central Kashmir valley and to identify risk variables linked to this infection. In addition to collecting 462 serum samples (244 males and 218 females), a questionnaire was also filled out for each participant utilizing systematic random sampling from June to November 2019 in 16 villages and 6 districts. Using a commercial enzyme-linked immunosorbent assay (ELISA) kit, the samples were tested for immunoglobulin G. Of the 462 serum samples obtained, 33 (7.14 percent) were found to be positive. Results showed that age group, gender, contact with dogs, occupation, and literacy rate were significantly related with the rate of seroprevalence of cystic echinococcosis infection in Central and North Kashmir. Statistics showed that the observations were significant (P< 0.05). Children, men, and those who were uneducated had considerably greater rates of seropositivity for this infection.
Effects on blood gas and haematological parameters during butorphanoldexmedetomidine- tiletamine-zolazepam anaesthesia in cats
Authors: Rahul B. , Venugopal Syam K. , Varghese Reji , Chandrasekhar Leena , Martin K. D. John
Published: April 2023

The present study was undertaken to evaluate the effects of butorphanol, dexmedetomidine, tiletamine and zolazepam anaesthesia on blood gas and haematological parameters in cats. These parameters were monitored before anaesthetic induction and 10 minutes following anaesthetic induction. Statistical analysis revealed that no significant changes exist and the parameters were within the normal reference range for cats. Therefore, this anaesthetic technique could be employed safely for elective surgery in cats.
Prevalence of endoparasitic infection in captive macaques in Kerala zoos
Authors: Raj V. L. Livin , Biju S. , Mathew Joseph , Zarina A.
Published: April 2023

The study was conducted in three macaque species namely Lion-tailed macaque (Macaca silenus), Rhesus macaque (Macaca mulatta) and Bonnet macaque (Macaca radiata) in the zoos of Kerala. Faecal samples were collected from individual macaques kept in open enclosures and retiring cages. The samples were examined by sedimentation method for assessing the endoparasitic infection and its prevalence percentage in captive macaques. The degree of infection was assessed by faecal egg counts of samples by McMaster technique. The study found zero prevalence of strongyles in Lion-tailed macaques; 20 per cent in Rhesus macaque and 100 per cent in Bonnet macaques. The eggs per gram of strongyles in Rhesus macaques were 100 ± 39.22 and in Bonnet macaques were 312 ± 47.94. The prevalence of coccidia was 16.6 per cent in Lion-tailed macaques; 40 per cent in Rhesus macaque and 100 per cent in Bonnet macaques. The oocysts per gram of coccidia in Lion-tailed macaques were 166 ± 98.88; in Rhesus macaques were 200 ± 89.44 and in Bonnet macaques were 1850 ± 53.54. The present study revealed the higher prevalence and degree of infection of coccidia and strongyles in Bonnet macaques when compared to the other two macaque species.
Pyothorax in a cat due to Acinetobacter spp. infection : a case report
Authors: Jameel A. Javed , Davis K. Justin , George Arun , Kumar K. Vinod , Udayasree V. J.
Published: April 2023

A 3-month-old kitten weighing 1.05 kg was presented to the University veterinary hospital at Kokkalai in Thrissur district of Kerala, India, with the complaint of open-mouth breathing, lethargy and complete anorexia for the past three days, and weight loss. Detailed clinical examination revealed inspiratory dyspnoea without stridor, tachypnoea, muffled lung and heart sounds on auscultation, pale mucous membrane, and weak pulse. Haematology revealed leukocytosis with granulocytosis and monocytosis. Thoracic radiography, ultrasonography and thoracocentesis confirmed pyothorax. Microbial culture of thoracic aspirate revealed heavy growth of Acinetobacte spp. The cat responded to parenteral therapy with ceftiofur followed by cefixime orally for 21 days. Doxycycline was prescribed for concurrent mycoplasmosis. Thoracic radiograph 10 days later revealed a significant reduction in pleural fluid. A review of the case two months later found that the kitten recovered uneventfully. A rare case of Acinetobacter spp. associated pyothorax and its medical management in a cat is reported. The clinical manifestations, radiographic changes and ultrasonographic findings were discussed.
Doppler ultrasonography of ventral perineal artery in bitches during different stages of oestrous cycle
Authors: Nikhila E. S. , Paul K. Magnus , Jayakumar C. , Aravind Amritha , Kurian Elizabeth
Published: April 2023

This study aimed to evaluate the Doppler ultrasonographic features of ventral perineal artery of bitches during different phases of oestrous cycle. Sixteen pluriparous bitches were subjected to transperineal Doppler ultrasonography during different stages of oestrous cycle by confirming the stage by exfoliative vaginal cytotlogy (EVC) and vaginoscopy. The resistivity index (RI), pulsatility index (PI), peak systolic velocity (PSV), end diastolic velocity (EDV) and systolic/ diastolic velocity (S/D) were analysed. No significant difference was found between the Doppler velocimetric parameters of ventral perineal artery during different phases of oestrous cycle by trasnperineal Doppler ultrasonography.
Molecular detection of Hepatozoon spp. in domestic cats of Kerala
Authors: Vincy P. , Tresamol P. V. , Davis K. Justin , Rajagopal Asha , Vijayakumar K.
Published: April 2023

Vector-borne diseases are an important cause of morbidity and mortality in domestic cat population and hepatozoonosis is one such infection. Incidence of hepatozoonosis has been described in a variety of animal species but information on cats is scarce. To investigate the occurrence of this pathogen in domestic cats, blood samples were collected from domestic cats from three districts of Kerala (Thrissur, Kannur and Wayanad). Field-stained blood smears were examined to detect the presence of gamonts of Hepatozoon spp. and the samples were subjected to molecular analysis by PCR amplification. Out of 122 blood samples screened, none of the sample revealed the presence of gamonts of Hepatozoon on microscopic examination. Polymerase chain reaction targeting 18S rRNA gene of Hepatozoon spp. demonstrated seven positive cases with a prevalence of 5.74 per cent. Results of this study indicate that hepatozoonosis is established within the domestic cats in Kerala and warrant the adoption of control measures.
Isolation, identification and antibiogram of coagulase negative staphylococci from bovine clinical mastitis
Authors: Jose Krupa Rose , Vijayakumar K.
Published: April 2023

The aim of this study was to identify the primary bacteria associated with clinical mastitis in dairy cows and to assess the antimicrobial resistance pattern of the commonest isolate using phenotypic methods. The milk samples were collected from 83 domesticated dairy cows suffering from clinical mastitis (CM), from an organised dairy farm as well as cows presented with CM at the University Veterinary Hospital (UVH), Mannuthy, Thrissur, Kerala. Microbiological analysis, using morphological, cultural and biochemical properties as well as molecular identification using polymerase chain reaction (PCR) revealed that coagulase-negative staphylococci (CNS) were the most predominant (40.31 per cent) bacteria, followed by Staphylococcus aureus (32.83 per cent), Micrococci spp. (11.94 per cent), Klebsiella spp. (7.46 per cent) Escherichia coli (4.48 per cent) and Streptococci (2.98 per cent). The majority of the bacteria were resistant to more than one class of antimicrobials (aminoglycosides, β lactams, fluoroquinolones or tetracyclines). Furthermore, our findings revealed that the CNS is highly resistant to β-lactam family of antibiotics and that CNS may play a significant role in clinical mastitis of dairy cows.
Assessment of quinolone resistance in bacteria isolated from corneal ulcers in dogs
Authors: Ashtamy M. G. , Mani B. K. , Mini M. , Ambily R. , Anoop S.
Published: April 2023

Corneal ulcers or ulcerative keratitis is the loss of continuity of corneal epithelium leading to exposure of corneal stroma. It is a highly painful condition in dogs. The common aetiological agents responsible for this condition are bacteria and for treatment, antibiotics are widely used. Indiscriminate and prolonged use of antibiotics can cause antimicrobial resistance (AMR) in bacteria. The present communication deals with the isolation of bacteria from corneal ulcers in dogs and the assessment of quinolone resistance. A total of 15 corneal swab samples were collected from dogs suffering from corneal ulcers, presented to the Department of Veterinary Surgery and Radiology, Teaching Veterinary Clinical Complex, College of Veterinary and Animal Sciences, Mannuthy. They were inoculated on to brain heart infusion agar as well as blood agar and incubated at 37 °C for 24 h for the isolation of aerobic bacteria. Eighteen isolates obtained were then subjected to antibiotic susceptibility test by disc diffusion method using the quinolone group of antibiotics, viz. ciprofloxacin, enrofloxacin, ofloxacin and moxifloxacin. Majority of the isolates exhibited varying resistance to these antibiotics. Polymerase chain reaction was performed to amplify gyrA gene, which is reported to be one of the reasons responsible for quinolone resistance with respect to its mutation. Seven out of the 18 isolates amplified gyrA gene. The amplicon obtained corresponding to a representative sample, which was found resistant by disc diffusion method was sequenced and a single point mutation was detected.
Linear foreign body in the small intestine of a Persian cat-a case report
Authors: Muheiri Maitha Al , Mohteshamuddin Khaja , Mahel Zaib , Ayub Azhar
Published: April 2023

Foreign body in gastrointestinal tract is a common problem in small animal practice, especially in domestic cats. A case report of recurrent ingested thread as a linear foreign body with the thread held back by the tongue and stuck causing further complications is presented in this case report. Interestingly, the radiographic results did not indicate the paisley shaped gas pattern which is characteristic of a linear foreign body. The final diagnosis was made by exploratory laparotomy which confirmed the presence of several threads in the small intestine. Therefore, this complex case was diagnosed based on history, clinical signs and results of radiography and confirmed by exploratory laparotomy. This is the first ever published case report of successful clinical management of a linear foreign body in UAE according to the authors’ knowledge.
Expression of Angiopoietin 2 in ovarian follicles of crossbred Malabari goat
Authors: Adithian M.S. , Babitha V. , Uma R. , Sajitha I. S. , Navya E. S.
Published: April 2023

Angiopoietin-2 (Ang-2) is a local ovarian growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signalling pathway, one of the main pathways involved in ovarian follicular angiogenesis. The main objective of this study is to assess the differential protein expression of Ang-2 in the developmental phases of ovarian follicles of crossbred Malabari goats employing an immunohistochemical technique. Ang-2 protein expression was found in all developmental phases of ovarian follicles, including primordial, primary, small, and large antral ovarian follicles. Ang-2 was found in the granulosa cells, theca interna cells, follicular fluid (granulosa cells in the FF), endothelial cells of capillaries and germinal epithelium of the goat ovaries. The Quantitative Method-Percentage Positivity of Ang-2 expression revealed that it was higher in the initial stages of ovarian follicular development and decreased with later stages of follicular development in cross-bred Malabari goats. Moreover, the protein expression of Ang2 was found to be higher in granulosa cells (GC) than in theca interna (TI) cells of antral follicles as evident from the higher staining intensity in GC cells compared to TI cells. It is therefore concluded that the Ang-2 protein expression in ovarian follicular cells decreased with an increase in follicle size and development and based on the combined scores for immunostaining (staining intensity and the number of positive staining cells), the Ang-2 was found to be expressed more in the granulosa cell layer compared to the theca interna cells of the antral follicle in crossbred Malabari goats.
Cryotolerance of in vitro matured and vitrified bovine oocytes supplemented with L-carnitine and niacin
Authors: Anjitha K. , Amritha A. , Abhilash R. S. , Jayakumar C. , Marykutty T.
Published: April 2023

The study was designed to compare the cryotolerance of in vitro matured bovine oocytes, supplemented with L- carnitine and niacin in maturation media, vitrified using cryoloop technique. Bovine ovaries were collected from the slaughterhouse and cumulus-oocyte complexes (COCs) were retrieved by follicular aspiration. Culture quality COCs were selected and randomly allocated to three different experimental groups. Group I oocytes (control), were subjected to in vitro maturation (IVM) in basic maturation media, while Group II and III oocytes (experimental groups) were matured in media supplemented with L- carnitine (0.6 mg/mL) and niacin (400 μM/mL), respectively. The mean per cent of cumulus cell expansion was 80.92 ±0.02, 79.18 ± 0.03 and 87.95 ±0.02 for Group I, II and III, respectively. A significantly high (p < 0.05) cumulus cell expansion could be observed in niacin treated group than L- carnitine, whereas a difference close to being statistically significant (p = 0.057) was noticed between niacin treated and control groups. The matured oocytes were subjected to cryoloopvitrification using dimethylsulfoxide (15%), ethylene glycol (15%) and sucrose (0.05M) as cryoprotectants. The mean survival rate observed in the study was 67.26 ± 0.05, 76.34 ± 0.05 and 79.68 ± 0.03 per cent for Group I, II and III, respectively. Shrinkage of cytoplasm was the most common type of cryodamage observed in all the three groups followed by cracked zona pellucida, abnormal shape and increased peri vitelline space. The survived oocytes were subjected to in vitro fertilisation with no significant difference between the groups in fertilisation rate. The present study revealed the beneficiary effect of supplementing niacin over L- carnitine in maturation media as it improved the maturation and survival rates of bovine oocytes.
Haematology profile of dogs with primary uterine inertia
Authors: Magnus P.K , Jayakumar C. , Thomas Naicy , Abhilash R. S. , Hiron M H. , Ajithkumar S.
Published: April 2023

Primary uterine inertia is the inherent inability of the uterus to contract and expel a fully grown foetus after the end of the gestation period, through a normal birth canal, in the absence of obstructive dystocia.There are conflicting reports regarding the blood parameters in parturient animals,and animals with dystocia and its role in uterine inertia are not well documented. Haematology analysis was performed in dogs with Complete Primary Uterine Inertia (CPUI, n=9) and Partial Primary Uterine Inertia (PPUI, n=6), as well as control animals with the Foetal Cause of Dystocia (FCD, n=7).Blood samples were collected from the study population and total leucocyte count (TLC), total erythrocyte count (TEC), differential leucocyte count (DLC), erythrocytic indices, haemoglobin and haematocrit values were estimated in an autoanalyzer. Haematology analysis revealed no significant difference between the groups. Differential leucocyte counts exhibited lymphocytosis across the groups suggestive of the active immune response related to CL lysis,a characteristic associated with the termination of the pregnancy in canines. Erythrocyte count, haemoglobin concentration and haematocrit revealed anaemia across the group.The study characterised the haematology profile of prepartum canines, which indicated anaemia associated with haemodilution and lymphocytosis associated with active immune status. It also proved that the haematology profile doesnot have any significant role in the pathogenesis of canine uterine inertia.
Effect of turmeric on performance of Arbor Acres plus strain of broiler chicken
Authors: Sunmola T. A. , Tuleun C. D.
Published: April 2023

This study was conducted to investigate the performance response of Arbor Acres plus strain of broiler chickens fed turmeric treated diets. A total of 120 day old broiler chicks were allocated to four dietary treatments, with three replicates, having 10 birds per replicate. The dietary treatments consisted of the control diet (T1) with 0 % turmeric powder and T2, T3 and T4 with turmeric powder added @ 0.20, 0.25 and 0.30 % of feed respectively. Results showed that average final weight and daily weight gain were not significantly influenced (P>0.05) across the treatments. The average daily feed intake and feed conversion ratio of the broilers differed significantly (P<0.05) amongst the treatment groups. Turmeric supplementation showed a significant decrease in feed conversion ratio and in turn improved feed efficiency. Dietary turmeric had no significant effect on nutrient utilization across the dietary groups. Significantly higher dressing percentage was obtained in broiler group fed 0.30 % turmeric powder while other carcass cuts and internal organs did not differ significantly. Turmeric powder had no significant effect on total protein, albumin, globulin and glucose, whereas, dietary effects of turmeric on AST, ALT and ALP decreased significantly in 0.30 % turmeric fed group. It was concluded that turmeric at 0.30 % had a positive influence on feed conversion ratio, dressing percentage and oxidative stress indicators in broiler chickens. Therefore, inclusion of turmeric powder at 0.30 % is recommended.
HUBUNGAN PERAWATAN PAYUDARA IBU PADA MASA POST PARTUM DENGAN MENYUSUI EKSKLUSIF DI PUSKESMAS ULU MORO’O NIAS BARAT TAHUN 2022
Authors: Gulo Hendrika Hartati , Novita Regina Vidya Trias , Simbolon Anna Rejeki
Published: April 2023

The WHO recommends that exclusive breastfeeding for six months must be carried out worldwide because it has benefits for both mother and baby, and is the baby's right to get breast milk. This study aims to determine whether there is a relationship between maternal breast care in the postpartum period and exclusive breastfeeding at the Ulu Moro'o Health Center, West Nias. This research is a quantitative study using an analytic design with a cross-sectional approach. The number of 60 respondents is based on the Slovin formula, with a total sampling in December 2021. Data collection uses a questionnaire about characteristics and breast care. The results showed that the majority (90%) were female reproductive age 20-35 years old, household work (91.7%), multiparous (61.7%), normal delivery (90%), and breastfeeding history was not the first experience (78.3%). The results of the chi-square test showed that there was no relationship between breast care (massage p= 0.467; warm or cold compresses p= 0.601) of mothers during the postpartum period with exclusive breastfeeding, so it was concluded that breastfeeding problems could not only be overcome by breast care such as massage or breastfeeding. However, it requires the cooperation of health workers in making skin-to-skin contact and IMD which is the first step in maintaining breastfeeding. The Puskesmas is expected to provide alternatives or other methods of breast care as well as education at least twice during pregnancy to mothers according to government regulations and intensive assistance to mothers in carrying out breast care education.
HUBUNGAN MENIKAH USIA ANAK TERHADAP KEJADIAN STUNTING PADA BALITA DI WONOSOBO
Authors: Mustajab Abdullah azam , Indriani Farihah
Published: April 2023

Stunting merupakan permasalahan gizi kronis yang disebabkan banyak faktor salah satunya pernikahan usia anak. Pernikahan usia anak masih banyak terjadi terutama di daerah pedesaan, rumah tangga dengan pengeluaran rendah dan pendidikan yang rendah. Penelitian ini bertujuan untuk menganalisis hubungan antara pernikahan usia anak terhadap kejadian stunting pada balita. Metode penelitian menggunakan survei analitik dengan pendekatan cross-sectional, orangtua balita dibagikan kuesioner untuk mengetahui usia pernikahan dan balita dilakukan pengukuran antropometri tinggi badan dengan standar WHO indeks TB/U, kategori stunting balita dengan TB/U < -2 SD. Sampel menggunakan total sampling didapatkan 161 balita dengan 56 balita stunting. Analisis data menggunakan analisis univariat dan bivariat untuk mengetahui hubungan antar variabel yang diteliti. Penelitian mendapatkan hasil dari uji chi square p value < 0,05 yang berarti terdapat hubungan yang signifikan antara pernikahan usia anak terhadap kejadian stunting pada balita dengan PR menunjukkan menikah usia anak berisiko mengalami stunting 1,984 kali dibandingkan yang tidak menikah usia anak 95%CI sebesar 1,243-3,168. Kesimpulan terdapat hubungan yang signifikan antara menikah usia anak terhadap kejadian stunting pada balita. Saran penlitian selanjutnya melakukan intervensi untuk menurunkan angka kejadian stunting terutama yang berkaitan dengan pernikahan usia anak.
ANALISIS PARAMETER HEMODINAMIK NON-INVASIF DENGAN KEJADIAN MORTALITAS PASIEN COVID-19 DI RUANG ICU
Authors: Utami Gamya Tri , Barbara Laksita
Published: April 2023

Hemodinamik tidak stabil sering ditemukan pada pasien kritis yang mendapat perawatan di ruang intensif salah satunya pasien dengan Covid-19. Apabila gangguan hemodinamik pasien tidak segera ditangani serta intervensi yang tidak dilakukan tepat waktu maka dapat berdampak terhadap otcome pasien seperti kegagalan organ hingga kematian. Penelitian ini bertujuan untuk menganalisis pengaruh parameter hemodinamik non-invasif terhadap kejadian mortalitas pasien Covid-19 yang mendapatkan perawatan di ICU. Penelitian yang menggunakan metode cohort retrospektif dengan 127 responden yang diambil dari rekam medis pasien menggunakan purposive sampling. Data hasil penelitian dianalisis menggunakan analisis univariat yang meliputi usia, jenis kelamin, length of stay (LoS), gambaran klinik hemodinamik, dan kejadian mortalitas, serta analisis multivariat menggunakan regrasi logistic. Hasil penelitian menunjukkan bahwa variabel suhu, tekanan darah sistolik, MAP, dan saturasi oksigen (pValue 0,000; 0,044; 0,001; 0,000; pValue< 0,05) merupakan variabel predictor yang paling berpengaruh signifikan terhadap kejadian mortalitas pasien Covid-19. Berdasarkan hasil penelitian, disarankan agar perawat dan tenaga medis lainnya melakukan monitoring hemodinamik dengan baik dalam upaya penetapan diagnosis yang akurat, penentuan pengobatan yang tepat, dan monitoring terhadap respon pengobatan.Kata Kunci: Co v id - 1 9 , I CU,  m o r t a l i t a s , parameter hemodinamik
KECEMASAN DAN KUALITAS TIDUR USIA DEWASA DI PANDEMI COVID-19
Authors: Tobing Duma Lumban , Haryani Ariyana Pramitha
Published: April 2023

ABSTRAK
Pandemi Covid-19 adalah wabah penyakit baru  di seluruh dunia dan menyebar dengan sangat cepat. Akibatnya, banyak orang menderita kecemasan berlebihan yang memengaruhi kualitas tidurnya. Tujuan dari penelitian ini untuk mengidentifikasi hubungan tingkat kecemasan masyarakat dengan kualitas tidur pada masa pandemi Covid-19. Penelitian ini menggunakan model korelasi cross sectional untuk mengetahui hubungan antara dua variabel yaitu bebas kecemasan dan kualitas tidur selama pandemi Covid-19. Data dikumpulkan dengan observasi langsung menggunakan Hamilton Anxiety Rating Scale Instrument dan Pittsburgh Sleep Quality Index. Sampel dalam penelitian ini terdiri dari 103 responden yang diambil dengan menggunakan teknik purposive sampling. Analisis data yang digunakan adalah analisis univariat dan bivariat dengan menggunakan uji chi-square. Hasil penelitian menunjukkan adanya hubungan kecemasan umum dengan kualitas tidur selama pandemi Covid-19 (p-value = 0,000). Penelitian ini akan membantu memperluas pengetahuan dan keterampilan kita untuk mengelola stres dan meningkatkan kualitas tidur.
Kata kunci: COVID 19, Kecemasan, Kualitas Tidur
MOTIVASI KLIEN DENGAN KEPATUHAN MINUM OBAT PADA PENDERITA HIPERTENSI
Authors: Adyani Sang Ayu Made , Mella Mahardika
Published: April 2023

Hypertension is a non-communicable disease  and is one of the highest causes of mobirdity and mortality in the world. Conditions like this require treatment for a long time and even a lifetime, so the successful treatment of this disease requires compliance in treatment.  The purpose of this study was to determine the relationship between client motivation and compliance with hypertension in taking medication.  The design of this study is descriptives with a cross sectional approach and data analysis using the chi-square test. The number of samples used was 144 respondents through a purposive sampling technique. The research instruments used the TMQ (Treatment Motivation Questionnaire) questionnaire, and MMAS-8 (Morisky Medication Adherence Scale).  The results of the study found that there was arelationship between client motivation and adherence to taking medication with p-value = 0.000 (p <0.005). This shows that there is a relationship between client motivation and medication adherence in hypertension sufferers Self-motivation is needed for clients so that hypertension therapy can be achieved according to the target.
AÇÕES DE AUTOCUIDADO REALIZADAS POR ADULTOS EM CUIDADOS PALIATIVOS: REVISÃO INTEGRATIVA
Authors: Moreira Gomes Anthony , Santana de Araújo Suelayne , Farias de Queiroz Frazão Cecília Maria , Galdino Albuquerque Perrelli Jaqueline , Brandão de Carvalho Lira Ana Luisa
Published: April 2023

RESUMO Objetivo: Identificar ações de autocuidado realizadas por adultos em cuidados paliativos. Método: Revisão integrativa, realizada nas bases de dados: MEDLINE, CINAHL, SCOPUS, Web of Science e LILACS, por meio do cruzamento dos descritores “cuidados paliativos”, “cuidados paliativos na terminalidade da vida”, “atitude frente à saúde”, “cuidado terminal”, “morte”, “autocontrole” e “autocuidado” e as respectivas traduções em inglês. O recorte temporal usado nas buscas foi entre 2017 e 2021, sendo a amostra final composta por 15 artigos. Resultados: Observou a prevalência de estudos com nível VI de evidência e rigor metodológico nível A. Encontraram-se 21 ações de autocuidado em todos os aspectos multidimensionais (físico, psicológico, social e espiritual). Conclusão: As diretivas antecipadas de vontade e a expressão do sentimento de esperança foram as mais predominantes. Essas, quando realizadas, foram capazes de garantir a autonomia do paciente e, consequentemente, a dignidade.
CONHECIMENTO DEFICIENTE DE PACIENTES SUBMETIDOS À HEMODIÁLISE: REVISÃO INTEGRATIVA
Authors: De Sousa Ramon Silva , Gomes Grande Marcela Eduarda , De Medeiros Tayná Martins , De Lima Laís Batista , De Sá Tinôco Jéssica Dantas , Farias de Queiroz de Frazão Cecilia Maria
Published: April 2023

RESUMO Objetivo: identificar os antecedentes e consequentes do diagnóstico de enfermagem Conhecimento Deficiente em pacientes submetidos à hemodiálise. Método: revisão integrativa, cuja busca aos artigos foi realizada nas bases de dados: BIREME, CINAHL, SCOPUS, Web of Science e LILACS. O recorte temporal usado nas buscas foi entre 2018 e 2022, sendo a amostra final composta por 12 artigos. Resultados: dentre os manuscritos selecionados, observou-se a prevalência de estudos com nível VI de evidência e rigor metodológico nível A. Os antecedentes identificados foram relacionados ao sexo masculino, à idade avançada, baixa escolaridade, falta de companheiro (a), baixo nível socioeconômico, inexistência de intervenções educativas para pacientes e ausência de memória. E os consequentes identificados foram relacionados à falta de adesão terapêutica, à presença de agravos e menor qualidade de vida. Conclusão: identificaram-se os antecedentes e consequentes do diagnóstico de enfermagem Conhecimento Deficiente em pacientes submetidos à hemodiálise, para auxiliar o profissional a inferir um diagnóstico de enfermagem de forma acurada.
Semi-Squat Exercises with Varying Levels of Arterial Occlusion Pressure during Blood Flow Restriction Training Induce a Post-Activation Performance Enhancement and Improve Vertical Height Jump in Female Football Players
Authors: Sun Depeng , Yang Tieli
Published: April 2023

Low-load blood flow restriction training (BFRT) has been shown to induce a significant increase in muscle activation. However, low-load BFRT to augment the post-activation performance enhancement (PAPE) has not been previously examined. This study aimed to examine the PAPE of low-intensity semi-squat exercises with varying pressure BFRT on vertical height jump performance. Twelve elite athletes from the Shaanxi Province women’s football team volunteered to participate in this study for 4 weeks. Participants completed four testing sessions that included one of the following at random: (1) non-BFRT, (2) 50% arterial occlusion pressure (AOP), (3) 60% AOP, or (4) 70% AOP. Muscle activity of the lower thigh muscles was recorded using electromyography (EMG). Jump height, peak power output (PPO), vertical ground reaction forces (vGRF), and rate of force development (RFD) were recorded for four trials. Two-factor repeated measures analysis of variance (ANOVA) showed that semi-squat with varying pressure BFRT had a significant impact on the measured muscle EMG amplitude and MF value of vastus medialis, vastus lateralis, rectus femoris, and biceps femoris (P < 0.05), and MF value decreased with increasing pressure. Muscle activation (EMG amplitude) did not change further. The EMG amplitude of the gluteus maximus was significantly decreased by semi-squat training with different pressures (P < 0.05), while that of the gluteus maximus muscle was gradually increased by non-BFR with semi-squat training (P > 0.05). The 50% and 60% AOP BFRTs significantly increased jump height, peak power, and force increase rate (RFD) after 5 min and 10 min of rest (P < 0.05). This study further confirmed that low-intensity BFRT can significantly increase lower limb muscle activation, induce PAPE, and improve vertical height jump in female footballers. In addition, 50% AOP continuous BFRT is recommended for warm-up activities.
ECONOMIC ASPECTS OF INNOVATIVE DEVELOPMENT OF ANIMAL HUSBANDRY IN MODERN CONDITIONS
Authors: Егембердиев Р.А. , Есиркепова А.М. , Жакипбекова Д.С. , Yegemberdiyev R.A. , Zhakipbekova D.S. , Yessirkepova A.M.
Published: April 2023

Мақалада инновациялық технологияларға бейімделуді ескере отырып, мал шаруашылығын дамытудың экономикалық тетіктері қарастырылады. Мақала авторлары жануарларды электрондық сәйкестендіру, автоматтандыру және робототехника саласында ғылыми зерттеулер кешенін жүргізу, фермерлік шаруашылықтардың тиімділігін арттыру үшін технологияларды пайдалану, сондай-ақ мал шаруашылығында инновацияны қолданудың аймақтық ерекшеліктері мәселелерін қарастырған ғалымдардың еңбектерін кеңінен зерттеген. Авторлар мал шаруашылығын мемлекеттік қолдау бойынша статистикалық деректерді талдау мен сараптауға көп көңіл бөлген. Талдау барысында мақала авторлары мал шаруашылығы саласындағы инновациялық дамудың мынадай тежеуші факторларын анықтаған: мемлекеттік субсидиялау жүйесіндегі сыбайлас жемқорлық; жайылым үшін жер учаскелерінің жетіспеушілігі; өндірістегі меншікті айналым және негізгі құралдарының аздығы; әлеуетті инвесторлар үшін мал шаруашылығы саласының тартымсыздығы; инновациялық технологияларды әзірлеу бойынша ғылыми әлеуеттің төмен деңгейі. Мақалада мал шаруашылығын инновациялық дамытуды мемлекеттік қолдауды жетілдіру жолдары айқындалған: қашықтан мониторинг технологиясы негізінде жайылымдарды басқару жөніндегі пәрменді жоспарларды әзірлеу; импортты алмастыруға және өнім экспортын дамытуға бағытталған жобаларды іске асыруды қаржылық қамтамасыз ету; мал шаруашылығы өнімдерін делдалдарсыз өткізу жөнінде шаралар қабылдау; аграрлық нарықта маманданған инвесторларды тарту жөнінде шаралар көздеу.

The article discusses the economic mechanisms of wildlife development, taking into account adaptation to innovative technologies. The author's articles consider the issues of electronic identification of the Living, conducting a complex of scientific research in the field of automation and robotics, the use of technologies to improve the efficiency of farms, as well as regional features of the application of innovations in animal husbandry. The authors have allocated a large amount to the analysis of statistical data on state support of the population. In the course of the analysis, the authors of the article identified the constraining factors of innovative development within the framework of state subsidies; non-appearance of land plots for grazing; shortage of own working capital and fixed assets; unattractiveness of activities for potential investors; low level of scientific potential for the development of innovative technologies. The article defines the ways of development of state support for innovative development: to develop existing plans for pasture management based on remote monitoring technologies; to ensure the implementation of projects aimed at importing and developing exports of products; to take measures to sell food products without intermediaries; to provide measures to attract investors, specializing in the agricultural market.
РАЗВИТИЕ ВНУТРЕННЕГО ТУРИЗМА В КАЗАХСТАНЕ: ОПРЕДЕЛЕНИЕ БАРЬЕРОВ
Authors: Abdrakhmanova S. , Umirzakov S. , Moghavvemi S. , Абдрахманова С.Е. , Умирзаков С.Ы. , Могхаввеми С.
Published: April 2023

INTERNAL TOURISM DEVELOPMENT IN KAZAKHSTAN: DETERMINATION OF THE BARRIERS Kazakhstan has significant potential for the development of the different types of tourism: from ecological to business. This is facilitated by a rich history that has left many archaeological and historical monuments on the territory of the country. This is ensured by the unique geographical position, thanks to which in Kazakhstan you can find untouched corners of the nature in a wide variety of landscapes. This is also achieved thanks to the development of tourism infrastructure, constant work to simplify the visa regime and many other factors. Despite the high potential of tourism sector in the Republic of Kazakhstan and richness in culture, resources, tourism has not reached the highest level of its development. Thus, this study aims to analyze the state of tourism development in the Republic of Kazakhstan in modern conditions, to determine what h

Казахстан имеет значительный потенциал для развития различных видов туризма: от экологического до делового. Этому способствует богатая история, оставившая на территории страны множество археологических и исторических памятников. Это обеспечивается уникальным географическим положением, благодаря которому в Казахстане можно найти нетронутые уголки природы в самых разнообразных ландшафтах. Это также достигается благодаря развитию туристской инфраструктуры, постоянной работе по упрощению визового режима и многим другим факторам. Несмотря на высокий потенциал туристской сферы Республики Казахстан и богатство культуры, ресурсов, туризм не достиг наивысшего уровня своего развития.
THE VAR MODEL IN ASSESSING THE CURRENCY RISKS OF KAZAKHSTAN'S SECOND-TIER BANKS
Authors: Мадыханова К.А. , Садыкова Р.Б. , Естурлиева А.И. , Саубетова Б.С. , Маdykhanova K. , Sadykova R. , Yesturliyeva A. , Saubetova B.
Published: April 2023

Мақаланың мақсаты – «Value at Risk» (VAR) әдістемесін зерттеу, оның мәнін, түсінігін және қолданудың негізгі аспектілерін зерттеу. Бұл бақылаудың тек сандық өлшеуге болатын параметрлермен ғана мүмкін болатындығына байланысты. Ал ықтимал қаржылық шығындарды басқару, кәсіпорынның, атап айтқанда, екінші деңгейлі банктердің шығындарын өтеу резервтерін анықтау үшін тәуекелді сандық бағалау шұғыл қажет. Осылайша, мақалада қазақстандық коммерциялық банктердің (Еуразиялық банк» АҚ мысалында) валюталық тәуекелдерін бағалау үшін «Value at Risk» (VAR) әдістемесін пайдалану мүмкіндігі карастырылған. Қазақстан Республикасы Ұлттық Банкінің талаптарына сәйкес Еуразиялық банктің ашық валюталық позициясының лимиті үшін VAR есебі ұсынылған. Мақалада тәуекел деңгейін есептеу кезінде мемлекетдегі макроэкономикалық жағдай да ескеріледі, оның ішінде қарастырылып отырған кезеңдегі теңге бағамының өзгеру динамикасы. «Еуразиялық банк» АҚ кредит портфелінің валюталық тәуекелінің деңгейін талдағанда, ҚР Ұлттық Банктің талаптарына сәйкес Еуразиялық банктің ашық валюталық позициясының (ұзақ және қысқа) лимиті үшін VAR есептелді. Сонымен қатар, болашақта туындайтын қаржылық шығындар және залалдың максималды деңгейі анықталды, ал ол өз кезегінде есептелген лимиттен аспауы керек. Талдаудың нәтижесінде VAR валюталық тәуекелінің ашық валюталық позиция шегіндегі шекті рұқсат етілген мәні анықталып, есептеулер негізінде қорытынды жасалды.

The purpose of management is that it is possible only over what can be quantified. Quantitative risk assessment is urgently needed to manage possible losses and determine reserves for loss insurance. The article reveals the concept of «Value at Risk», highlights the issues of the possibility of using the Value at Risk (VaR) methodology to assess the currency risks of Kazakhstani commercial banks (using the example of JSC «Eurasian Bank»). The calculation of VAR for the limit of the open currency position of the Eurasian Bank according to the requirements of the National Bank of the Republic of Kazakhstan is presented. Possible financial losses have been identified. Therefore, the analysis focuses on the reality of fulfilling the condition, the maximum level of losses that should not exceed the calculated limit. When calculating the risk level, the article also considers the macroeconomic situation in the state, including the dynamics of changes in the tenge exchange rate for the period under review. The analysis of the level of currency risk of the loan portfolio of JSC «Eurasian Bank» has been compiled. The VAR level is calculated for the limit of the open currency position (long and short) of the Eurasian Bank in accordance with the requirements of the National Bank of the Republic of Kazakhstan. The maximum permissible value of currency risk VAR for the limit of an open currency position has been determined. A summary based on calculations is summarized.