bluuebunny/crossref_metadata_2025 huggingface dataset:
The Complex Issue of Urban Trees—Stress Factor Accumulation and Ecological Service Possibilities
Authors: Czaja Monika , Kołton Anna , Muras Piotr
Published: August 2020

This review paper is the first that summarizes many aspects of the ecological role of trees in urban landscapes while considering their growth conditions. Research Highlights are: (i) Plant growth conditions in cities are worsening due to high urbanization rates and new stress factors; (ii) Urban trees are capable of alleviating the stress factors they are exposed to; (iii) The size and vitality of trees is related to the ecological services they can provide. Our review shows, in a clear way, that the phenomenon of human-related environmental degradation, which generates urban tree stress, can be effectively alleviated by the presence of trees. The first section reviews concerns related to urban environment degradation and its influence on trees. Intense urbanization affects the environment of plants, raising the mortality rate of urban trees. The second part deals with the dieback of city trees, its causes and scale. The average life expectancy of urban trees is relatively low and depends on factors such as the specific location, proper care and community involvement, among others. The third part concerns the ecological and economic advantages of trees in the city structure. Trees affect citizen safety and health, but also improve the soil and air environment. Finally, we present the drawbacks of tree planting and discuss if they are caused by the tree itself or rather by improper tree management. We collect the latest reports on the complicated state of urban trees, presenting new insights on the complex issue of trees situated in cities, struggling with stress factors. These stressors have evolved over the decades and emphasize the importance of tree presence in the city structure.
Modelling of Impact Falling Ball Test Response on Solid and Engineered Wood Flooring of Two Eucalyptus Species
Authors: Acuña Luis , Sepliarsky Fernando , Spavento Eleana , Martínez Roberto D. , Balmori José-Antonio
Published: August 2020

In this study, dynamic hardness tests on solid and engineered wood flooring specimens of Eucalyptus globulus Labill. and Eucalyptus grandis W. Hill ex Maiden hardwoods were performed because nowadays, these fast-growing hardwoods are still scarcely employed for this use. Furthermore, another two examples of hardwood commonly applied on wood flooring, Quercus robur L. and Hymenaea courbaril L., were also tested. To compare their properties, a dynamic impact hardness test based on the impact of steel balls, with several diameters, and drop heights was developed. Accordingly, 120 solid wood flooring specimens and 120 engineering wood flooring specimens were producing with these four hardwood species. Dynamic impact tests were made with three steel balls of different diameters (30–40–50 mm), and they were carried out from five different drop heights (0.60–0.75–0.90–1.05–1.20 m). The impact of the steel ball drew the size of the footprint on the surface and this mark was measured with a digital caliper for both dimensions, diameter and depth, as footprint diameter (FD) and indentation depth (ID). Data from 3000 samples, corresponding to 120 different individual groups (4 species × 3 ball diameters × 5 drop height × 2 floor type) were analyzed. Results indicated that the variability of ID (CV between 19.25–25.61%) is much greater than the values achieved for FD (CV between 6.72–7.91%). Regarding the fast-growing hardwood species tested, E. globulus showed a similar behavior to traditional hardwood applied on wood flooring in Europe, Q. robur, and it could be a promising growth in the flooring industry. However, E. grandis showed the worst values compared to traditional hardwood in all test configurations.
Potential Impact of Climate Change on the Forest Coverage and the Spatial Distribution of 19 Key Forest Tree Species in Italy under RCP4.5 IPCC Trajectory for 2050s
Authors: Pecchi Matteo , Marchi Maurizio , Moriondo Marco , Forzieri Giovanni , Ammoniaci Marco , Bernetti Iacopo , Bindi Marco , Chirici Gherardo
Published: August 2020

Forests provide a range of ecosystem services essential for human wellbeing. In a changing climate, forest management is expected to play a fundamental role by preserving the functioning of forest ecosystems and enhancing the adaptive processes. Understanding and quantifying the future forest coverage in view of climate changes is therefore crucial in order to develop appropriate forest management strategies. However, the potential impacts of climate change on forest ecosystems remain largely unknown due to the uncertainties lying behind the future prediction of models. To fill this knowledge gap, here we aim to provide an uncertainty assessment of the potential impact of climate change on the forest coverage in Italy using species distribution modelling technique. The spatial distribution of 19 forest tree species in the country was extracted from the last national forest inventory and modelled using nine Species Distribution Models algorithms, six different Global Circulation Models (GCMs), and one Regional Climate Models (RCMs) for 2050s under an intermediate forcing scenario (RCP 4.5). The single species predictions were then compared and used to build a future forest cover map for the country. Overall, no sensible variation in the spatial distribution of the total forested area was predicted with compensatory effects in forest coverage of different tree species, whose magnitude and patters appear largely modulated by the driving climate models. The analyses reported an unchanged amount of total land suitability to forest growth in mountain areas while smaller values were predicted for valleys and floodplains than high-elevation areas. Pure woods were predicted as the most influenced when compared with mixed stands which are characterized by a greater species richness and, therefore, a supposed higher level of biodiversity and resilience to climate change threatens. Pure softwood stands along the Apennines chain in central Italy (e.g., Pinus, Abies) were more sensitive than hardwoods (e.g., Fagus, Quercus) and generally characterized by pure and even-aged planted forests, much further away from their natural structure where admixture with other tree species is more likely. In this context a sustainable forest management strategy may reduce the potential impact of climate change on forest ecosystems. Silvicultural practices should be aimed at increasing the species richness and favoring hardwoods currently growing as dominating species under conifers canopy, stimulating the natural regeneration, gene flow, and supporting (spatial) migration processes.
Easy-To-Interpret Procedure to Analyze Fire Seasonality and the Influence of Land Use in Fire Occurrence: A Case Study in Central Italy
Authors: Bajocco Sofia , Ferrara Carlotta , Guglietta Daniela , Ricotta Carlo
Published: August 2020

Fire frequency and fire seasonality are among the main components of the fire regime. In the Mediterranean Basin, climate directly drives fire occurrence, controlling fuel flammability and determining the fire-prone conditions, so that intense fires prevail during the dry and warm season of the year. However, humans also play a direct role in wildfire regimes, severely altering fuel features, fire policies and land-use management, as well as the timing and location of fire ignitions, to such an extent that anthropogenic activities have overcome the role of climate in shaping fire regimes. The main purpose of this work is to propose a graphical tool capable of identifying the most fire-prone portions of the territory and to explore the differences between the summer and winter fire risk; to this end, we analyzed the seasonal fire risk in the Latium region (central Italy) and its drivers in terms of land-use types, by using a fuel phenology framework. The results demonstrated that climate is not the main cause of bimodal seasonality in fire occurrence and that the existence of two annual fire seasons in Latium is strongly correlated with how humans use fire as a land management tool. The proposed approach may represent an easy-to-interpret pyrogeographical framework applicable in any environment and updatable over time, useful for identifying spatial gradients, and for recognizing fire regime temporal patterns.
Nonlinear Model-Based Inferential Control of Moisture Content of Spray Dried Coconut Milk
Authors: Abdullah Zalizawati , Taip Farah Saleena , Kamal Siti Mazlina Mustapa , Rahman Ribhan Zafira Abdul
Published: August 2020

The moisture content of a powder is a parameter crucial to be controlled in order to produce stable products with a long shelf life. Inferential control is the best solution to control the moisture content due to difficulty in measuring this variable online. In this study, fundamental and empirical approaches were used in designing the nonlinear model-based inferential control of moisture content of coconut milk powder that was produced from co-current spray dryer. A one-dimensional model with integration of reaction engineering approach (REA) model was used to represent the dynamic of the spray drying process. The empirical approach, i.e., nonlinear autoregressive with exogenous input (NARX) and neural network, was used to allow fast and accurate prediction of output response in inferential control. Minimal offset (<0.0003 kg/kg) of the responses at various set points indicate high accuracy of the neural network estimator. The nonlinear model-based inferential control was able to provide stable control response at wider process operating conditions and acceptable disturbance rejection. Nevertheless, the performance of the controller depends on the tuning rules used.
Red Shrimp Are a Rich Source of Nutritionally Vital Lipophilic Compounds: A Comparative Study among Edible Flesh and Processing Waste
Authors: Saini Ramesh Kumar , Song Min-Ho , Rengasamy Kannan R. R. , Ko Eun-Young , Keum Young-Soo
Published: August 2020

This study was aimed at comparatively analyzing the sterols, tocopherols and fatty acids from edible flesh and processing waste obtained from three shrimp species, utilizing rapid liquid chromatography (LC)-atmospheric-pressure chemical ionization (APCI)-tandem mass spectrometry (MS/MS) and gas chromatography-mass spectrometry (GC-MS). Results revealed the presence of significantly (p < 0.05) high proportions of health-beneficial omega-3 (n3) polyunsaturated fatty acids (PUFAs) in Argentine red shrimp (34.3% in waste and 38.2% in the flesh), compared to black tiger shrimp (16.5–24.2%) and whiteleg shrimp (13.2–22.6%). Among sterols, cholesterol was found most dominant, accounting in the range 349.4 (white shrimp flesh) to 559.3 µg/g fresh weight (FW) (black shrimp waste). Surprisingly, waste was found to contain a substantially higher amount of α-tocopherol, for instance, 21.7 µg/g FW in edible flesh and 35.3 µg/g FW in the waste of black tiger shrimp. The correlation analysis indicated that shrimp with low total contents of lipids might have higher proportions of health-beneficial long-chain (LC)-n3-PUFAs eicosapentaenoic (EPA) and docosahexaenoic acid (DHA). The fat quality indices, including the high ratios of hypocholesterolemic (h)/hypercholesterolemic (H) fatty acids, and lowest values of the atherogenic index (AI) and thrombogenic index (TI) indicated the health-beneficial potential associated with fat intake from red shrimp. Overall, a significant amount of health-beneficial compounds in edible flesh of studied shrimp confers its extraordinary nutritional benefits. Moreover, considering the richness of processing waste with these compounds, their valorization can be prompted.
Place of Residence and Marital Status as Variables Differentiating a Sense of Self-Efficacy in the Elderly—A Descriptive Cross-Sectional Survey
Authors: Jakubowska Klaudia , Wysokiński Mariusz , Chruściel Paweł
Published: August 2020

Background: Studies on the self-efficacy of the elderly are still being conducted to a very limited extent. Nevertheless, they can provide relevant information for specialists to use in many disciplines of science, as well as for practitioners, especially gerontologists, geriatricians, psychologists and other people associated with prophylaxis and health promotion. The aim of the study is to assess the impact of the place of residence and marital status on the self-efficacy in the elderly. Methods: A diagnostic survey method was used for the study and within the method, the authors’ sociodemographic data questionnaire and the generalized self-efficacy scale (GSES) were applied. The study was carried out in a group of 171 females and 130 males under nursing care in the home environment or in nursing-home care in Poland. Results: The average level of generalized sense of self-efficacy in the research cohort was M = 26.69, SD = 8.49. A relatively high percentage of the group (48.9%) showed a sense of self-efficacy at a high level. The level of a sense of self-efficacy was low in 38.2% of the cohort, while its average intensity in the remaining ones constituted 12.9% of the total. A statistically significant impact of a place of residence (Z = −2.940; p = 0.003) and marital status (H = 12.000; p = 0.007) on the sense of efficacy in the research cohort was proven in favor of those from the rural environment and the respondents having spouses. Conclusions: On the scale of the studied group, the results of a sense of self-efficacy are optimistic, as older people with high levels predominated. However, the results of the cohort whose self-efficacy is insufficient to cope with their old age should be considered carefully, especially in the case of lonely people living in the urban environment and NHC (nursing-home care) residents.
Evaluation of the Effectiveness of the Use of the Diode Laser in the Reduction of the Volume of the Edematous Gingival Tissue after Causal Therapy
Authors: Polizzi Elisabetta , Tetè Giulia , Targa Claudia , Salviato Barbara , Ferrini Francesco , Gastaldi Giorgio
Published: August 2020

Aim: The aim of this study was to evaluate and compare two different techniques for the treatment of plaque-induced gingivitis, demonstrating whether the causal therapy supported by diode laser can allow a resolution of the edema caused by gingivitis in less time compared to the single traditional causal therapy. Materials and methods: Twenty-five patients between 20 and 60 years of age with a specific diagnosis of gingivitis were evaluated at the CLID-HSR oral hygiene department. Once the clinical parameters (bleeding index, plaque index, recession, and clinical attack level) were recorded, each of them was subjected to a professional oral hygiene session and instructed in correct home hygiene procedures. Through a split-mouth protocol for each individual patient, hemi-arches were treated by simple randomization to be treated with causal therapy supported by the action of the diode laser (experimental therapy) and which with traditional causal therapy (control therapy). A first intraoral scan was performed before therapy (T0), which was repeated 20 min after rinsing with CHX. The intraoral scans were repeated at a control 7 (T1) and 14 days (T2) after the session. For each intraoral scan, a volumetric value was calculated, proportional to the edema of the gingival tissues, using special digital software. The operator who carried out the volumetric measurements on the software was not aware of the therapy implemented on each half-arch. The operator who carried out the statistical analysis was not aware of the therapy applied to each group. The collected data were statistically compared in order to detect any differences between the volumetric variations between the two therapy groups and within the therapy groups over time. After evaluating the distribution of data by means of the Kolmogorov-Smirnov statistical test, the appropriate nonparametric tests were chosen to carry out the statistical comparisons. Results: Based on the analysis of the gingival-periodontal health parameters and the volumetric value of the treated areas, no statistically significant differences were detected between the areas treated with the adjuvant action of the diode laser compared to those treated with causal therapy alone. Conclusions: With the limitations of this study, in accordance with the statistical results obtained, diode laser therapy does not allow a faster resolution of gingival edema compared to traditional therapy; the two treatment techniques for plaque-induced gingivitis, therefore, have the same efficacy.
Sport Promotion through Sport Mega-Events. An Analysis for Types of Olympic Sports in London 2012
Authors: Kokolakakis Themistocles , Lera-Lopez Fernando
Published: August 2020

A substantial amount of attention has been devoted towards the potential sport legacy of the Olympic Games. In spite of the increasing academic interest in this topic, there is a knowledge gap as far as sport legacy is concerned by types of different sports. The authors bridge this gap by analysing the evolution of 43 different Olympic/Paralympic sport modalities in the two-year period after the London 2012 Olympics. By using data from the Active People Survey with a sample of 165,000 people annually, and considering some demographic variables and the effect of the economic environment, the paper aims to test the existence of a sport legacy. We have applied time series analysis and ARIMA models for controlling for economic influence and seasonal adjustment and for making comparisons among participation rates. The results show, for the total of the sports analysed, that there were 336,000 individuals who increased their frequency of participation, while there was no significant increase in the number of new participants in these sports. When we develop the analysis for types of sports, London 2012 is positively associated not only with the frequency of participation in some types of sport but also with an increase in the number of new sport participants. Gender and age differences are also detected. The results show the differences of sport legacy by type of sports. Moreover, this research has elucidated an important unrecognised aspect of the effect of the Olympic Games and perhaps major events: that they can become a major policy tool for reversing sporting inequalities.
Photosystem Disorder Could be the Key Cause for the Formation of Albino Leaf Phenotype in Pecan
Authors: Zhang Ji-Yu , Wang Tao , Jia Zhan-Hui , Guo Zhong-Ren , Liu Yong-Zhi , Wang Gang
Published: August 2020

Pecan is one of the most famous nut species in the world. The phenotype of mutants with albino leaves was found in the process of seeding pecan, providing ideal material for the study of the molecular mechanisms leading to the chlorina phenotype in plants. Both chlorophyll a and chlorophyll b contents in albino leaves (ALs) were significantly lower than those in green leaves (GLs). A total of 5171 differentially expression genes (DEGs) were identified in the comparison of ALs vs. GLs using high-throughput transcriptome sequencing; 2216 DEGs (42.85%) were upregulated and 2955 DEGs (57.15%) were downregulated. The expressions of genes related to chlorophyll biosynthesis (HEMA1, encoding glutamyl-tRNA reductase; ChlH, encoding Mg-protoporphyrin IX chelatase (Mg-chelatase) H subunit; CRD, encoding Mg-protoporphyrin IX monomethylester cyclase; POR, encoding protochlorophyllide reductase) in ALs were significantly lower than those in GLs. However, the expressions of genes related to chlorophyll degradation (PAO, encoding pheophorbide a oxygenase) in ALs were significantly higher than those in GLs, indicating that disturbance of chlorophyll a biosynthesis and intensification of chlorophyll degradation lead to the absence of chlorophyll in ALs of pecan. A total of 72 DEGs associated with photosynthesis pathway were identified in ALs compared to GLs, including photosystem I (15), photosystem II (19), cytochrome b6-f complex (3), photosynthetic electron transport (6), F-type ATPase (7), and photosynthesis-antenna proteins (22). Moreover, almost all the genes (68) mapped in the photosynthesis pathway showed decreased expression in ALs compared to GLs, declaring that the photosynthetic system embedded within the thylakoid membrane of chloroplast was disturbed in ALs of pecan. This study provides a theoretical basis for elucidating the molecular mechanism underlying the phenotype of chlorina seedlings of pecan.
Artificial Light at Night Increases Recruitment of New Neurons and Differentially Affects Various Brain Regions in Female Zebra Finches
Authors: Moaraf Stan , Heiblum Rachel , Vistoropsky Yulia , Okuliarová Monika , Zeman Michal , Barnea Anat
Published: August 2020

Despite growing evidence that demonstrate adverse effects of artificial light at night (ALAN) on many species, relatively little is known regarding its effects on brain plasticity in birds. We recently showed that although ALAN increases cell proliferation in brains of birds, neuronal densities in two brain regions decreased, indicating neuronal death, which might be due to mortality of newly produced neurons or of existing ones. Therefore, in the present study we studied the effect of long-term ALAN on the recruitment of newborn neurons into their target regions in the brain. Accordingly, we exposed zebra finches (Taeniopygia guttata) to 5 lux ALAN, and analysed new neuronal recruitment and total neuronal densities in several brain regions. We found that ALAN increased neuronal recruitment, possibly as a compensatory response to ALAN-induced neuronal death, and/or due to increased nocturnal locomotor activity caused by sleep disruption. Moreover, ALAN also had a differential temporal effect on neuronal densities, because hippocampus was more sensitive to ALAN and its neuronal densities were more affected than in other brain regions. Nocturnal melatonin levels under ALAN were significantly lower compared to controls, indicating that very low ALAN intensities suppress melatonin not only in nocturnal, but also in diurnal species.
T Cell Immunity to Bacterial Pathogens: Mechanisms of Immune Control and Bacterial Evasion
Authors: Shepherd Freya R. , McLaren James E.
Published: August 2020

The human body frequently encounters harmful bacterial pathogens and employs immune defense mechanisms designed to counteract such pathogenic assault. In the adaptive immune system, major histocompatibility complex (MHC)-restricted αβ T cells, along with unconventional αβ or γδ T cells, respond to bacterial antigens to orchestrate persisting protective immune responses and generate immunological memory. Research in the past ten years accelerated our knowledge of how T cells recognize bacterial antigens and how many bacterial species have evolved mechanisms to evade host antimicrobial immune responses. Such escape mechanisms act to corrupt the crosstalk between innate and adaptive immunity, potentially tipping the balance of host immune responses toward pathological rather than protective. This review examines the latest developments in our knowledge of how T cell immunity responds to bacterial pathogens and evaluates some of the mechanisms that pathogenic bacteria use to evade such T cell immunosurveillance, to promote virulence and survival in the host.
Derlin-3 Is Required for Changes in ERAD Complex Formation under ER Stress
Authors: Eura Yuka , Miyata Toshiyuki , Kokame Koichi
Published: August 2020

Endoplasmic reticulum (ER)-associated protein degradation (ERAD) is a quality control system that induces the degradation of ER terminally misfolded proteins. The ERAD system consists of complexes of multiple ER membrane-associated and luminal proteins that function cooperatively. We aimed to reveal the role of Derlin-3 in the ERAD system using the liver, pancreas, and kidney obtained from different mouse genotypes. We performed coimmunoprecipitation and sucrose density gradient centrifugation to unravel the dynamic nature of ERAD complexes. We observed that Derlin-3 is exclusively expressed in the pancreas, and its deficiency leads to the destabilization of Herp and accumulation of ERAD substrates. Under normal conditions, Complex-1a predominantly contains Herp, Derlin-2, HRD1, and SEL1L, and under ER stress, Complex-1b contains Herp, Derlin-3 (instead of Derlin-2), HRD1, and SEL1L. Complex-2 is upregulated under ER stress and contains Derlin-1, Derlin-2, p97, and VIMP. Derlin-3 deficiency suppresses the transition of Derlin-2 from Complex-1a to Complex-2 under ER stress. In the pancreas, Derlin-3 deficiency blocks Derlin-2 transition. In conclusion, the composition of ERAD complexes is tissue-specific and changes in response to ER stress in a Derlin-3-dependent manner. Derlin-3 may play a key role in changing ERAD complex compositions to overcome ER stress.
The Structure of Clostridioides difficile SecA2 ATPase Exposes Regions Responsible for Differential Target Recognition of the SecA1 and SecA2-Dependent Systems
Authors: Lindič Nataša , Loboda Jure , Usenik Aleksandra , Vidmar Robert , Turk Dušan
Published: August 2020

SecA protein is a major component of the general bacterial secretory system. It is an ATPase that couples nucleotide hydrolysis to protein translocation. In some Gram-positive pathogens, a second paralogue, SecA2, exports a different set of substrates, usually virulence factors. To identify SecA2 features different from SecA(1)s, we determined the crystal structure of SecA2 from Clostridioides difficile, an important nosocomial pathogen, in apo and ATP-γ-S-bound form. The structure reveals a closed monomer lacking the C-terminal tail (CTT) with an otherwise similar multidomain organization to its SecA(1) homologues and conserved binding of ATP-γ-S. The average in vitro ATPase activity rate of C. difficile SecA2 was 2.6 ± 0.1 µmolPi/min/µmol. Template-based modeling combined with evolutionary conservation analysis supports a model where C. difficile SecA2 in open conformation binds the target protein, ensures its movement through the SecY channel, and enables dimerization through PPXD/HWD cross-interaction of monomers during the process. Both approaches exposed regions with differences between SecA(1) and SecA2 homologues, which are in agreement with the unique adaptation of SecA2 proteins for a specific type of substrate, a role that can be addressed in further studies.
Environmental Signal-Dependent Regulation of Flowering Time in Rice
Authors: Shim Jae Sung , Jang Geupil
Published: August 2020

The transition from the vegetative to the reproductive stage of growth is a critical event in the lifecycle of a plant and is required for the plant’s reproductive success. Flowering time is tightly regulated by an internal time-keeping system and external light conditions, including photoperiod, light quality, and light quantity. Other environmental factors, such as drought and temperature, also participate in the regulation of flowering time. Thus, flexibility in flowering time in response to environmental factors is required for the successful adaptation of plants to the environment. In this review, we summarize our current understanding of the molecular mechanisms by which internal and environmental signals are integrated to regulate flowering time in Arabidopsis thaliana and rice (Oryza sativa).
Amino Acid Transporters as Targets for Cancer Therapy: Why, Where, When, and How
Authors: Bröer Stefan
Published: August 2020

Amino acids are indispensable for the growth of cancer cells. This includes essential amino acids, the carbon skeleton of which cannot be synthesized, and conditionally essential amino acids, for which the metabolic demands exceed the capacity to synthesize them. Moreover, amino acids are important signaling molecules regulating metabolic pathways, protein translation, autophagy, defense against reactive oxygen species, and many other functions. Blocking uptake of amino acids into cancer cells is therefore a viable strategy to reduce growth. A number of studies have used genome-wide silencing or knock-out approaches, which cover all known amino acid transporters in a large variety of cancer cell lines. In this review, these studies are interrogated together with other databases to identify vulnerabilities with regard to amino acid transport. Several themes emerge, such as synthetic lethality, reduced redundancy, and selective vulnerability, which can be exploited to stop cancer cell growth.
A De novo Peptide from a High Throughput Peptide Library Blocks Myosin A -MTIP Complex Formation in Plasmodium falciparum
Authors: Anam Zill e , Joshi Nishant , Gupta Sakshi , Yadav Preeti , Chaurasiya Ayushi , Kahlon Amandeep Kaur , Kaushik Shikha , Munde Manoj , Ranganathan Anand , Singh Shailja
Published: August 2020

Apicomplexan parasites, through their motor machinery, produce the required propulsive force critical for host cell-entry. The conserved components of this so-called glideosome machinery are myosin A and myosin A Tail Interacting Protein (MTIP). MTIP tethers myosin A to the inner membrane complex of the parasite through 20 amino acid-long C-terminal end of myosin A that makes direct contacts with MTIP, allowing the invasion of Plasmodium falciparum in erythrocytes. Here, we discovered through screening a peptide library, a de-novo peptide ZA1 that binds the myosin A tail domain. We demonstrated that ZA1 bound strongly to myosin A tail and was able to disrupt the native myosin A tail MTIP complex both in vitro and in vivo. We then showed that a shortened peptide derived from ZA1, named ZA1S, was able to bind myosin A and block parasite invasion. Overall, our study identified a novel anti-malarial peptide that could be used in combination with other antimalarials for blocking the invasion of Plasmodium falciparum.
Production of a Granulysin-Based, Tn-Targeted Cytolytic Immunotoxin Using Pulsed Electric Field Technology
Authors: Guerrero-Ochoa Patricia , Aguilar-Machado Diederich , Ibáñez-Pérez Raquel , Macías-León Javier , Hurtado-Guerrero Ramón , Raso Javier , Anel Alberto
Published: August 2020

Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors. Previous work demonstrated the therapeutic effect of the intratumoral injection of recombinant granulysin and of the systemic injection of an immunotoxin between granulysin and the anti-carcinoembryonic antigen single-chain Fv antibody fragment MFE23, which were produced in the yeast Pichia pastoris. In the present work, we developed a second immunotoxin combining granulysin and the anti-Tn antigen single-chain Fv antibody fragment SM3, that could have a broader application in tumor treatment than our previous immunotoxin. In addition, we optimized a method based on electroporation by pulsed electric field (PEF) to extract the remaining intracellular protein from yeast, augmenting the production and purificiation yield. The immunotoxin specifically recognized the Tn antigen on the cell surface. We also compared the thermal stability and the cytotoxic potential of the extracellular and intracellular immunotoxins on Tn-expressing human cell lines, showing that they were similar. Moreover, the bioactivity of both immunotoxins against several Tn+ cell lines was higher than that of granulysin alone.
Neuroenergetics and “General Intelligence”: A Systems Biology Perspective
Authors: Debatin Tobias
Published: August 2020

David C. Geary proposed the efficiency of mitochondrial processes, especially the production of energy, as the most fundamental biological mechanism contributing to individual differences in general intelligence (g). While the efficiency of mitochondrial functioning is undoubtedly an important and highly interesting factor, I outline several reasons why other main factors of neuroenergetics should not be neglected and why a systems biology perspective should be adopted. There are many advantages for research on intelligence to focus on individual differences in the capability of the overall brain metabolism system to produce the energy currency adenosine triphosphate (ATP): higher predictive strength than single mechanisms, diverse possibilities for experimental manipulation, measurement with existing techniques and answers to unresolved questions because of multiple realizability. Many of these aspects are especially important for research on developmental processes and the building and refining of brain networks for adaptation. Focusing too much on single parts of the system, like the efficiency of mitochondrial functioning, carries the danger of missing important information about the role of neuroenergetics in intelligence and valuable research opportunities.
Numerical and Experimental Optimization Study on a Fast, Zero Emission Catamaran
Authors: Papanikolaou Apostolos , Xing-Kaeding Yan , Strobel Johannes , Kanellopoulou Aphrodite , Zaraphonitis George , Tolo Edmund
Published: August 2020

The present study focuses on the hydrodynamic hull form optimization of a zero emission, battery driven, fast catamaran vessel. A two-stage optimization procedure was implemented to identify in the first stage (global optimization) the optimum combination of a ship’s main dimensions and later on in the second stage (local optimization) the optimal ship hull form, minimizing the required propulsion power for the set operational specifications and design constraints. Numerical results of speed-power performance for a prototype catamaran, intended for operation in the Stavanger area (Norway), were verified by model experiments at Hamburgische Schiffbau Versuchsanstalt (HSVA), proving the feasibility of this innovative, zero emissions, waterborne urban transportation concept.
The Role of RodA-Conserved Cysteine Residues in the Aspergillus fumigatus Conidial Surface Organization
Authors: Valsecchi Isabel , Stephen-Victor Emmanuel , Wong Sarah Sze Wah , Karnam Anupama , Sunde Margaret , Guijarro J. Iñaki , Rodríguez de Francisco Borja , Krüger Thomas , Kniemeyer Olaf , Brown Gordon D. , Willment Janet A. , Latgé Jean-Paul , Brakhage Axel A. , Bayry Jagadeesh , Aimanianda Vishukumar
Published: August 2020

Immune inertness of Aspergillusfumigatus conidia is attributed to its surface rodlet-layer made up of RodAp, characterized by eight conserved cysteine residues forming four disulfide bonds. Earlier, we showed that the conserved cysteine residue point (ccrp) mutations result in conidia devoid of the rodlet layer. Here, we extended our study comparing the surface organization and immunoreactivity of conidia carrying ccrp-mutations with the RODA deletion mutant (∆rodA). Western blot analysis using anti-RodAp antibodies indicated the absence of RodAp in the cytoplasm of ccrp-mutant conidia. Immunolabeling revealed differential reactivity to conidial surface glucans, the ccrp-mutant conidia preferentially binding to α-(1,3)-glucan, ∆rodA conidia selectively bound to β-(1,3)-glucan; the parental strain conidia showed negative labeling. However, permeability of ccrp-mutants and ∆rodA was similar to the parental strain conidia. Proteomic analyses of the conidial surface exposed proteins of the ccrp-mutants showed more similarities with the parental strain, but were significantly different from the ∆rodA. Ccrp-mutant conidia were less immunostimulatory compared to ∆rodA conidia. Our data suggest that (i) the conserved cysteine residues are essential for the trafficking of RodAp and the organization of the rodlet layer on the conidial surface, and (ii) targeted point mutation could be an alternative approach to study the role of fungal cell-wall genes in host–fungal interaction.
Reoccupying Ecological Land for Excessively Expanded Rust Belt Cities in Traditional Grain Bases: An Eco-Economic Trade-Off Perspective
Authors: Liu Shuhan , Lei Guoping , Wang Dongyan , Li Hong , Li Wenbo , Gao Jia
Published: August 2020

The optimization of ecological resource allocation is increasingly seen as a potential solution for urban revitalization and sustainable land use planning, and the key point is to assess and simulate the spatial arrangement of the ecological land. In this study, we proposed a conceptual framework with the aim of reoccupying ecological resources for rust belt cities from the perspective of eco-economic trade-offs. The ecological security pattern, the urban development pattern, and the ecological quality of cropland were constructed and evaluated to measure the development level of an ecological system and a socio-economic system. Furthermore, the results were used as the constraints that influenced land use distribution to simulate the ecological land reoccupation pattern. The suitable area, the preservation area, the configurable area, and the unsuitable area in the reoccupation pattern accounted for 6.94%, 49.97%, 28.17%, and 0.69%, respectively. Significantly, under strict cropland protection policies, the available space for ecological land expansion was heavily compressed. Therefore, the emphasis on agricultural production should be reexamined to release more space for ecological resources. This method could be an effective pathway to alleviate the pressures on urban and natural space caused by the competition between land-use activities, such as economic development, agricultural production, and ecological conservation. The findings are expected to promote urban revitalization, green agriculture, and sustainable social development in rust belt cities, and provide certain references for the utilization of land resources and regional policy making.
Effect of Carbon Addition and Mixture Method on the Microstructure and Mechanical Properties of Silicon Carbide
Authors: Yaşar Zeynep Aygüzer , Haber Richard A.
Published: August 2020

High dense (>99% density) SiC ceramics were produced with addition of C and B4C by spark plasma sintering method at 1950 °C under 50 MPa applied pressure for 5 min. To remove the oxygen from the SiC, it was essential to add C. Two different mixture method were used, dry mixing (specktromill) and wet mixing (ball milling). The effect of different levels of carbon additive and mixture method on density, microstructure, elastic modulus, polytype of SiC, Vickers hardness, and fracture toughness were examined. Precisely, 1.5 wt.% C addition was sufficient to remove oxide layer from SiC and improve the properties of dense SiC ceramics. The highest hardness and elastic modulus values were 27.96 and 450 GPa, respectively. Results showed that the 4H polytype caused large elongated grains, while the 6H polytype caused small coaxial grains. It has been observed that it was important to remove oxygen to achieve high density and improve properties of SiC. Other key factor was to include sufficient amount of carbon to remove oxide layer. The results showed that excess carbon prevented to achieve high density with high elastic modulus and hardness.
Physico-Mechanical and Hygro-Thermal Properties of Compressed Earth Blocks Stabilized with Industrial and Agro By-Product Binders
Authors: Nshimiyimana Philbert , Messan Adamah , Courard Luc
Published: August 2020

This study investigated the engineering properties of compressed earth blocks (CEBs) stabilized with by-product binders: calcium carbide residue (CCR) and rice husk ash (RHA). The dry mixtures were prepared using the earthen material and 0–25 wt% CCR, firstly, and 20 wt% CCR partially substituted by the RHA (CCR:RHA in 20:0–12:8 ratios), secondly. The appropriate amount of water was thoroughly mixed with the dry mixtures. The moistened mixtures were manually compressed into CEBs, cured, dried, and tested. The stabilization of CEBs with CCR increased the dry compressive strength (CS) from 1.1 MPa with 0% CCR to 4.3 MPa with 10% CCR and above; decreased the bulk density (ρb: 1800–1475 kg/m3) and increased the total porosity (TP:35–45%). This resulted in the improvement of the coefficient of structural efficiency (CSE: 610–3050 Pa∙m3/kg). It also improved the thermal efficiency given the decrease of the thermal conductivity (λ: 1.02–0.69 W/m∙K), thermal diffusivity (a: 6.3 × 10−7 to 4.7 × 10−7 m2/s) and thermal penetration depth (δp: 0.13–0.11 m). The RHA further improved the CS up to 7 MPa, reaching the optimum with 16:4 CCR:RHA (ρb: 1575 kg/m3 and TP: 40%). The latter reached higher CSE (4460 Pa∙m3/kg) than cement stabilized CEBs (3540 Pa∙m3/kg). It reached lower λ (0.64 w/m∙K), a (4.1 × 10−7 m2/s) and δp (0.11 m) than cement CEBs (1.01 w/m∙K, 6.8 × 10−7 m2/s, and 0.14 m). Additionally, the stabilization of CEBs with by-products improved the moisture sorption capacity. The improvement of the structural and thermal efficiency of CEBs by the stabilization with by-product binders is beneficial for load-bearing capacity and thermal performances in multi-storey buildings.
Writing 3D Nanomagnets Using Focused Electron Beams
Authors: Fernández-Pacheco Amalio , Skoric Luka , De Teresa José María , Pablo-Navarro Javier , Huth Michael , Dobrovolskiy Oleksandr V.
Published: August 2020

Focused electron beam induced deposition (FEBID) is a direct-write nanofabrication technique able to pattern three-dimensional magnetic nanostructures at resolutions comparable to the characteristic magnetic length scales. FEBID is thus a powerful tool for 3D nanomagnetism which enables unique fundamental studies involving complex 3D geometries, as well as nano-prototyping and specialized applications compatible with low throughputs. In this focused review, we discuss recent developments of this technique for applications in 3D nanomagnetism, namely the substantial progress on FEBID computational methods, and new routes followed to tune the magnetic properties of ferromagnetic FEBID materials. We also review a selection of recent works involving FEBID 3D nanostructures in areas such as scanning probe microscopy sensing, magnetic frustration phenomena, curvilinear magnetism, magnonics and fluxonics, offering a wide perspective of the important role FEBID is likely to have in the coming years in the study of new phenomena involving 3D magnetic nanostructures.
First-Degree Prime Ideals of Biquadratic Fields Dividing Prescribed Principal Ideals
Authors: Santilli Giordano , Taufer Daniele
Published: August 2020

We describe first-degree prime ideals of biquadratic extensions in terms of the first-degree prime ideals of two underlying quadratic fields. The identification of the prime divisors is given by numerical conditions involving their ideal norms. The correspondence between these ideals in the larger ring and those in the smaller ones extends to the divisibility of specially-shaped principal ideals in their respective rings, with some exceptions that we explicitly characterize.
A Concise Route for the Synthesis of Tetracyclic Meroterpenoids: (±)-Aureol Preparation and Mechanistic Interpretation
Authors: Rosales Martínez Antonio , Enríquez Lourdes , Jaraíz Martín , Pozo Morales Laura , Rodríguez-García Ignacio , Díaz Ojeda Emilio
Published: August 2020

A new concise general methodology for the synthesis of different tetracyclic meroterpenoids is reported: (±)-aureol (1), the key intermediate of this general route. The synthesis of (±)-aureol (1) was achieved in seven steps (28% overall yield) from (±)-albicanol. The key steps of this route include a C–C bond-forming reaction between (±)-albicanal and a lithiated arene unit and a rearrangement involving 1,2-hydride and 1,2-methyl shifts promoted by BF3•Et2O as activator and water as initiator.
Stability Performance Analysis of Various Packaging Materials and Coating Strategies for Chronic Neural Implants under Accelerated, Reactive Aging Tests
Authors: Gong Yan , Liu Wentai , Wang Runyu , Brauer Matthew Harris , Zheng Kristine , Li Wen
Published: August 2020

Reliable packaging for implantable neural prosthetic devices in body fluids is a long-standing challenge for devices’ chronic applications. This work studied the stability of Parylene C (PA), SiO2, and Si3N4 packages and coating strategies on tungsten wires using accelerated, reactive aging tests in three solutions: pH 7.4 phosphate-buffered saline (PBS), PBS + 30 mM H2O2, and PBS + 150 mM H2O2. Different combinations of coating thicknesses and deposition methods were studied at various testing temperatures. Analysis of the preliminary data shows that the pinholes/defects, cracks, and interface delamination are the main attributes of metal erosion and degradation in reactive aging solutions. Failure at the interface of package and metal is the dominating factor in the wire samples with open tips.
Tick-Borne Pathogens Shape the Native Microbiome Within Tick Vectors
Authors: Adegoke Abdulsalam , Kumar Deepak , Bobo Cailyn , Rashid Muhammad Imran , Durrani Aneela Zameer , Sajid Muhammad Sohail , Karim Shahid
Published: August 2020

Ticks are blood-feeding arthropods and transmit a variety of medically important viral, bacterial, protozoan pathogens to animals and humans. Ticks also harbor a diverse community of microbes linked to their biological processes, such as hematophagy, and hence affect vector competence. The interactions between bacterial and/or protozoan pathogens and the tick microbiome is a black-box, and therefore we tested the hypothesis that the presence of a protozoan or bacterial pathogen will alter the microbial composition within a tick. Hence, this study was designed to define the microbial composition of two tick species, Hyalomma (H.) anatolicum and Rhipicephalus (R.) microplus. We used a combination of PCR based pathogen (Anaplasma marginale and Theileria species) and symbiont (Wolbachia species) identification followed by metagenomic sequencing and comparison of the microbial communities in PCR positive and negative ticks. A total of 1786 operational taxonomic units was identified representing 25 phyla, 50 classes, and 342 genera. The phylum Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota were the most represented bacteria group. Alpha and beta diversity were not significantly affected in the presence or absence of Theileria sp. and A. marginale as see with H. anatolicum ticks. Interestingly, bacterial communities were significantly reduced in Theileria sp. infected R. microplus ticks, while also exhibiting a significant reduction in microbial richness and evenness. Putting these observations together, we referred to the effect the presence of Theileria sp. has on R. microplus a “pathogen-induced dysbiosis”. We also identify the presence of Plasmodium falciparum, the causative agent of human malaria from the microbiome of both H. anatolicum and R. microplus ticks. These findings support the presence of a “pathogen-induced dysbiosis” within the tick and further validation experiments are required to investigate how they are important in the vector competence of ticks. Understanding the mechanism of “pathogen-induced dysbiosis” on tick microbial composition may aid the discovery of intervention strategies for the control of emerging tick-borne infections.