bluuebunny/crossref_metadata_2025 huggingface dataset:
Passive rifting and continental splitting in the Jurassic Ligurian Tethys: the mantle perspective
Authors: Piccardo Giovanni B.
Published: November 2014

Based on present knowledge of mantle peridotites from the Ligurian Tethys ophiolites, this paper presents new ideas and a new model for passive rifting to ocean spreading in the slow–ultraslow rifting Europe–Adria realm. Relevant points include: (i) the positive feedback between deformation and melt percolation during passive magmatic rifting; (ii) the positive feedback between natural evidence and experimental data on the behaviour of the mantle lithosphere during passive rifting; (iii) the significance of hidden magmatism and the associated melt thermal advection; (iv) the role of the wedge-shaped weakened and softened axial zone; and (v) the evidence of a transition from passive to active rifting in the Ligurian Tethys.

Passive rifting induced passive asthenospheric upwelling and the onset of partial melting. Fractional melts migrated through the mantle lithosphere and stagnated at shallow levels (the hidden magmatism). Melt thermal advection heated the mantle lithosphere to temperatures (
T
) of ≥1200°C and formed a wedge-shaped axial zone of rheological softened/weakened mantle peridotites that served as the future locus of continental break-up. The hotter/deeper asthenosphere ascended within this axial zone, underwent partial melting and formed aggregated mid-ocean ridge basalts (MORBs) that migrated within dunite channels to form olivine gabbro intrusions and basaltic lava flows. Rifting evolved from passive to active, and the actively upwelling asthenosphere established a ridge-type system and thermal regime.
SirexWoodwasp: A Model for Evolving Management Paradigms of Invasive Forest Pests
Authors: Slippers Bernard , Hurley Brett P. , Wingfield Michael J.
Published: November 2014

The Sirex woodwasp, Sirex noctilio, and its fungal mutualist, Amylostereum areolatum, together constitute one of the most damaging invasive pests of pine. Despite a century of research and well-established management programs, control remains unpredictable and spread continues to new areas. Variable success in managing this pest has been influenced by complex invasion patterns, the multilayered nature of biological interactions, the varying local ecologies, and microevolutionary population processes in both the biocontrol organisms and in the wasps. Recent research findings are challenging the historical perspectives on methods to manage the Sirex woodwasp, calling for management programs to incorporate the variable local dynamics affecting this pest complex. In this regard, the Sirex woodwasp provides a superb model to illustrate the need for a different approach to develop efficient and sustainable management tools to deal with the growing and global nature of pest invasions in forests and plantations.
Metformin in Cancer Treatment and Prevention
Authors: Morales Daniel R. , Morris Andrew D.
Published: November 2014

Patients with diabetes mellitus are at increased risk of cancer development. Metformin is a well-established, effective agent for the management of type 2 diabetes mellitus. Epidemiological studies have identified an association between metformin use and a beneficial effect on cancer prevention and treatment, which has led to increasing interest in the potential use of metformin as an anticancer agent. Basic science has provided a better understanding of the mechanism of action of metformin and the potential for metformin to modulate molecular pathways involved in cancer cell signaling and metabolism. This article outlines the link between metformin and cancer, the potential for metformin in oncology, and limitations of currently available evidence.
A Clearer View of the Molecular Complexity of Clear Cell Renal Cell Carcinoma
Authors: Frew Ian J. , Moch Holger
Published: November 2014

The von Hippel–Lindau (VHL) tumor suppressor gene is mutated as an early event in almost all cases of clear cell renal cell carcinoma (ccRCC), the most frequent form of kidney cancer. In this review we discuss recent advances in understanding how dysregulation of the many hypoxia-inducible factor α–dependent and –independent functions of the VHL tumor suppressor protein (pVHL) can contribute to tumor initiation and progression. Recent evidence showing extensive inter- and intratumoral genetic diversity has given rise to the idea that ccRCC should actually be considered as a series of molecularly related, yet distinct, diseases defined by the pattern of combinatorial genetic alterations present within the cells of the tumor. We highlight the range of genetic and epigenetic alterations that recur in ccRCC and discuss the mechanisms through which these events appear to function cooperatively with a loss of pVHL function in tumorigenesis.
Hepatitis C Virus–Associated Cancer
Authors: Lin Ming V. , King Lindsay Y. , Chung Raymond T.
Published: November 2014

Hepatitis C virus (HCV) is one of the major etiologic agents of liver cancer. HCV is an RNA virus that, unlike hepatitis B virus, is unable to integrate into the host genome. Through complex interactions between viral and host proteins that induce host responses and promote inflammation, fibrosis, and ultimately cirrhosis, HCV infection can result in the development of hepatocellular carcinoma (HCC). The HCV oncogenic process involves genetic and epigenetic alterations and oncogenic effects mediated by viral proteins in the activation of cellular oncogenes, inactivation of tumor-suppressor genes, and dysregulation of multiple signal-transduction pathways. Advances in genetics and gene expression profiling have enhanced our current understanding of the pathways involved in HCV-associated liver cancer development. In this review, we summarize the current understanding of mechanisms of hepatocarcinogenesis induced by HCV infection.
SWI/SNF Chromatin Remodeling and Human Malignancies
Authors: Masliah-Planchon Julien , Bièche Ivan , Guinebretière Jean-Marc , Bourdeaut Franck , Delattre Olivier
Published: November 2014

The SWI/SNF complexes, initially identified in yeast 20 years ago, are a family of multi-subunit complexes that use the energy of adenosine triphosphate (ATP) hydrolysis to remodel nucleosomes. Chromatin remodeling processes mediated by the SWI/SNF complexes are critical to the modulation of gene expression across a variety of cellular processes, including stemness, differentiation, and proliferation. The first evidence of the involvement of these complexes in carcinogenesis was provided by the identification of biallelic, truncating mutations of the SMARCB1 gene in malignant rhabdoid tumors, a highly aggressive childhood cancer. Subsequently, genome-wide sequencing technologies have identified mutations in genes encoding different subunits of the SWI/SNF complexes in a large number of tumors. SWI/SNF mutations, and the subsequent abnormal function of SWI/SNF complexes, are among the most frequent gene alterations in cancer. The mechanisms by which perturbation of the SWI/SNF complexes promote oncogenesis are not fully elucidated; however, alterations of SWI/SNF genes obviously play a major part in cancer development, progression, and/or resistance to therapy.
The Role of Endoplasmic Reticulum Stress in Human Pathology
Authors: Oakes Scott A. , Papa Feroz R.
Published: November 2014

Numerous genetic and environmental insults impede the ability of cells to properly fold and posttranslationally modify secretory and transmembrane proteins in the endoplasmic reticulum (ER), leading to a buildup of misfolded proteins in this organelle—a condition called ER stress. ER-stressed cells must rapidly restore protein-folding capacity to match protein-folding demand if they are to survive. In the presence of high levels of misfolded proteins in the ER, an intracellular signaling pathway called the unfolded protein response (UPR) induces a set of transcriptional and translational events that restore ER homeostasis. However, if ER stress persists chronically at high levels, a terminal UPR program ensures that cells commit to self-destruction. Chronic ER stress and defects in UPR signaling are emerging as key contributors to a growing list of human diseases, including diabetes, neurodegeneration, and cancer. Hence, there is much interest in targeting components of the UPR as a therapeutic strategy to combat these ER stress–associated pathologies.
Agent-Based Models and Microsimulation
Authors: Heard Daniel , Dent Gelonia , Schifeling Tracy , Banks David
Published: November 2014

Agent-based models (ABMs) are computational models used to simulate the actions and interactions of agents within a system. Usually, each agent has a relatively simple set of rules for how he or she responds to his or her environment and to other agents. These models are used to gain insight into the emergent behavior of complex systems with many agents, in which the emergent behavior depends upon the micro-level behavior of the individuals. ABMs are widely used in many fields, and this article reviews some of those applications. However, as relatively little work has been done on statistical inference for such models, this article also points out some of those gaps and recent strategies to address them.
CYP2J2 overexpression attenuates nonalcoholic fatty liver disease induced by high-fat diet in mice
Authors: Chen Guangzhi , Xu Renfan , Zhang Shasha , Wang Yinna , Wang Peihua , Edin Matthew L. , Zeldin Darryl C. , Wang Dao Wen
Published: November 2014

Cytochrome P-450 epoxygenase-derived epoxyeicosatrienoic acids (EETs) exert diverse biological activities, which include potent vasodilatory, anti-inflammatory, antiapoptotic, and antioxidatant effects, and cardiovascular protection. Liver has abundant epoxygenase expression and high levels of EET production; however, the roles of epoxygenases in liver diseases remain to be elucidated. In this study, we investigated the protection against high-fat diet-induced nonalcoholic fatty liver disease (NAFLD) in mice with endothelial-specific CYP2J2 overexpression (Tie2-CYP2J2-Tr). After 24 wk of high-fat diet, Tie2-CYP2J2-Tr mice displayed attenuated NAFLD compared with controls. Tie2-CYP2J2-Tr mice showed significantly decreased plasma triglyceride levels and liver lipid accumulation, improved liver function, reduced inflammatory responses, and less increase in hepatic oxidative stress than wild-type control mice. These effects were associated with inhibition of NF-κB/JNK signaling pathway activation and enhancement of the antioxidant defense system in Tie2-CYP2J2-Tr mice in vivo. We also demonstrated that 14,15-EET treatment protected HepG2 cells against palmitic acid-induced inflammation and oxidative stress. 14,15-EET attenuated palmitic acid-induced changes in NF-κB/JNK signaling pathways, malondialdehyde generation, glutathione levels, reactive oxygen species production, and NADPH oxidase and antioxidant enzyme expression in HepG2 cells in vitro. Together, these results highlight a new role for CYP epoxygenase-derived EETs in lipotoxicity-related inflammation and oxidative stress and reveal a new molecular mechanism underlying EETs-mediated anti-inflammatory and antioxidant effects that could aid in the design of new therapies for the prevention and treatment of NAFLD.
Hypertrichotic Giant Nevus Spilus Tardivus and Neurofibroma of the Tongue in Sporadic von Recklinghausen’s Disease
Authors: Ramakrishnan Prabhath , Sylvester Vijay , Sreenivasan Prathima , Vengalath Janisha , Valambath Smruthi
Published: November 2014

Solitary neurofibromas are rare, benign tumours of nonodontogenic origin. The presentation of a solitary neurofibroma on the tongue is an uncommon occurrence and we present such a case here which was discovered in concomitance with multiple neurofibromatosis type 1 (von Recklinghausen’s disease). Such a rare presentation seen in this case is a diagnostic challenge and often clinched only with the aid of histopathological and immunohistochemical examination. This work also discusses the various differential diagnoses that can be considered in similar cases. The presence of a hypertrichotic “giant” nevus spilus tardivus (Becker’s nevus) is also a rare finding in this particular case. We present such a case which will be of interest to the budding dental practitioner. The lesion was excised and the patient followed up without any evidence of malignant transformation.
Evaluating the Individualized Treatment of Traditional Chinese Medicine: A Pilot Study of N‐of‐1 Trials
Authors: Huang Haiyin , Yang Peilan , Xue Jingjing , Tang Jie , Ding Liyu , Ma Ying , Wang Jie , Guyatt Gordon H. , Vanniyasingam Thuva , Zhang Yuqing
Published: November 2014

Purpose. To compare the efficacy of individualized herbal decoction with controlled decoction for individual patients with stable bronchiectasis. Methods. We conducted N‐of‐1 RCTs (single‐patient, double‐blind, randomized, multiple crossover design) in 3 patients with stable bronchiectasis. The primary outcome was patient self‐rated symptom scores on visual analogue scales. Secondary outcome was 24‐hour sputum volume. A clinical efficacy criterion which combined symptoms score and medication preference was also formulated. Results. All three patients showed various degrees of improvement on their symptoms and one patient’s (Case 3) 24 h sputum volume decreased from 70 mL to 30 mL. However, no significant differences were found between individualized herbal decoction and control decoction on symptoms score, or on 24‐hour sputum volume. One patient (Case 2) had clear preference for the individualized herbal decoction over the standard one with the confirmation after unblinding. We therefore considered this case as clinically important. Discussion. N‐of‐1 trials comply with individualized philosophy of TCM clinical practice and had good compliance. It is necessary to set up clinical efficacy criteria and to consider the interference of acute exacerbation.
A Case-Based Reasoning Method for Remanufacturing Process Planning
Authors: Zhou Fan , Jiang Zhigang , Zhang Hua , Wang Yan
Published: November 2014

Remanufacturing is a practice of growing importance due to its increasing environmental and economic benefits. Process planning plays a critical role in realizing a successful remanufacturing strategy. This paper presents a case-based reasoning method for remanufacturing process planning, which allows a process planner to rapidly retrieve, reuse, revise, and retain the solutions to past process problems. In the proposed method, influence factors including essential characteristics, failure characteristics, and remanufacturing processing characteristics are identified, and the local similarity of influence factors between the new case and the past cases is determined by nearest neighbor matching method, and then the vector of correction factor for local similarity is utilized in the nearest neighbor algorithm to improve the accuracy and effectiveness of case searching. To assess the usefulness and practicality of the proposed method, an illustrative example is given and the results are discussed.
Numerical Simulation of Nonperiodic Rail Operation Diagram Characteristics
Authors: Qian Yongsheng , Wang Bingbing , Zeng Junwei , Wang Xin
Published: November 2014

This paper succeeded in utilizing cellular automata (CA) model to simulate the process of the train operation under the four-aspect color light system and getting the nonperiodic diagram of the mixed passenger and freight tracks. Generally speaking, the concerned models could simulate well the situation of wagon in preventing trains from colliding when parking and restarting and of the real-time changes the situation of train speeds and displacement and get hold of the current train states in their departures and arrivals. Finally the model gets the train diagram that simulates the train operation in different ratios of the van and analyzes some parameter characters in the process of train running, such as time, speed, through capacity, interval departing time, and departing numbers.
Application of Temperature-Dependent Fluorescent Dyes to the Measurement of Millimeter Wave Absorption in Water Applied to Biomedical Experiments
Authors: Kuzkova Nataliia , Popenko Oleksandr , Yakunov Andrey
Published: November 2014

Temperature sensitivity of the fluorescence intensity of the organic dyes solutions was used for noncontact measurement of the electromagnetic millimeter wave absorption in water. By using two different dyes with opposite temperature effects, local temperature increase in the capillary that is placed inside a rectangular waveguide in which millimeter waves propagate was defined. The application of this noncontact temperature sensing is a simple and novel method to detect temperature change in small biological objects.
A Green Strategy for Federated and Heterogeneous Clouds with Communicating Workloads
Authors: Mateo Jordi , Vilaplana Jordi , Plà Lluis M. , Lérida Josep Ll. , Solsona Francesc
Published: November 2014

Providers of cloud environments must tackle the challenge of configuring their system to provide maximal performance while minimizing the cost of resources used. However, at the same time, they must guarantee an SLA (service-level agreement) to the users. The SLA is usually associated with a certain level of QoS (quality of service). As response time is perhaps the most widely used QoS metric, it was also the one chosen in this work. This paper presents a green strategy (GS) model for heterogeneous cloud systems. We provide a solution for heterogeneous job-communicating tasks and heterogeneous VMs that make up the nodes of the cloud. In addition to guaranteeing the SLA, the main goal is to optimize energy savings. The solution results in an equation that must be solved by a solver with nonlinear capabilities. The results obtained from modelling the policies to be executed by a solver demonstrate the applicability of our proposal for saving energy and guaranteeing the SLA.
Effect of Uncaria tomentosa Extract on Apoptosis Triggered by Oxaliplatin Exposure on HT29 Cells
Authors: de Oliveira Liliane Z. , Farias Iria Luiza G. , Rigo Melânia L. , Glanzner Werner G. , Gonçalves Paulo Bayard D. , Cadoná Francine C. , Cruz Ivana B. , Farias Júlia G. , Duarte Marta M. M. F. , Franco Luzia , Bertol Gustavo , Colpo Elisangela , Brites Patricia C. , Rocha João Batista T. , Leal Daniela B. R.
Published: November 2014

Background/Aim. The use of herbal products as a supplement to minimize the effects of chemotherapy for cancer treatment requires further attention with respect to the activity and toxicity of chemotherapy. Uncaria tomentosa extract, which contains oxindole alkaloids, is one of these herbal products. The objective of this study was to evaluate whether Uncaria tomentosa extract modulates apoptosis induced by chemotherapy exposure. Materials and Methods. Colorectal adenocarcinoma cells (HT29 cells) were grown in the presence of oxaliplatin and/or Uncaria tomentosa extract. Results. The hydroalcoholic extract of Uncaria tomentosa enhanced chemotherapy‐induced apoptosis, with an increase in the percentage of Annexin positive cells, an increase in caspase activities, and an increase of DNA fragments in culture of the neoplastic cells. Moreover, antioxidant activity may be related to apoptosis. Conclusion. Uncaria tomentosa extract has a role for cancer patients as a complementary therapy. Further studies evaluating these beneficial effects with other chemotherapy drugs are recommended.
Evaluating the Effect of Ramadan Fasting on Muslim Patients with Diabetes in relation to Use of Medication and Lifestyle Patterns: A Prospective Study
Authors: Siaw Melanie Yee Lee , Chew Daniel Ek Kwang , Dalan Rinkoo , Abdul Shakoor Shaikh Abdul Kader Kamaldeen , Othman Noorani , Choo Chor Hui , Shamsuri Nur Hidayah , Abdul Karim Siti Nurhana , Chan Sui Yung , Lee Joyce Yu-Chia
Published: November 2014

Objectives. This study aimed to examine the effect of Ramadan fasting on HbA1c in Muslim patients with type 2 diabetes. The incidence of hypoglycemia and glycemic changes in relation to the adjustment of doses of antidiabetic agents, diet, and physical activity during Ramadan was also evaluated.Methods. This was a prospective study conducted in an outpatient endocrine clinic. A set of questionnaires was administered to Muslim patients with diabetes who fasted for≥10 days. Those who were hospitalized for diabetic ketoacidosis or severe hypoglycemia a month prior to Ramadan or were given short-term corticosteroid therapy were excluded. The patients’ responses and clinical outcomes from the clinic database were collected before, during, and after Ramadan.Results. A total of 153 participants completed the study. The mean HbA1c improved from 8.9% before Ramadan to 8.6% during Ramadan (P<0.05). Although diet and physical activity did not contribute to changes in glycemia, a significant improvement in HbA1c was observed in patients who had adjustments made to their doses of antidiabetic agents during Ramadan (P<0.001). In addition, their rate of hypoglycemia was minimal.Conclusions. Ramadan fasting appeared to improve glycemic control, especially in those whose doses of antidiabetic agents were adjusted during Ramadan.
Entrepreneurship Education in Health Care Education
Authors: Salminen L. , Lindberg E. , Gustafsson M.-L. , Heinonen J. , Leino-Kilpi H.
Published: November 2014

This study describes the content of entrepreneurship education in health care education and the kinds of teaching methods that are used when teaching about entrepreneurship. Health care entrepreneurship has increased in many countries in recent decades and there is evidence that entrepreneurs have also a role in public health care. Therefore the health care professionals need to be educated to have the entrepreneurial skills. Education in the field of health care is still based on traditional forms of teaching and does not give enough attention to the issue of becoming an entrepreneur. The data was collected from teachers(n=111)via e-mail from six Finnish polytechnics. The data were analysed statistically and the open-ended questions were analysed via content analysis. Approximately 23% of the teachers had taught about entrepreneurship. The most popular teaching methods were company visits and cases, lecturing, and project work. The courses dealt with establishing a company, entrepreneurship in general, and marketing. Nearly all of the teachers had cooperated with the entrepreneurs or with the companies in question. Approximately 33% of the teachers took entrepreneurship into consideration often in other courses related to entrepreneurship.
Cold Plasma Pretreatment of Carbon Fibre Composite Substrates to Improve Adhesive Bonding Performance
Authors: Mandolfino Chiara , Lertora Enrico , Gambaro Carla
Published: November 2014

The purpose of the paper is to investigate the effects of low pressure plasma treatment on wettability of carbon fibre reinforced polymer samples and on shear properties of adhesive bonded joints based on these substrates. In particular, two plasma process parameters, exposure time and power input, were optimized, performing contact angle evaluation on lap-shear tests. The plasma treatment was also compared with a conventional mechanical abrasion and untreated and only degreased specimens. The experimental results show that choosing the optimal parameters is possible to improve the wettability of composite substrates and reduce the contact angle.
Field Equations and Radial Solutions in a Noncommutative Spherically Symmetric Geometry
Authors: Yazdani Aref
Published: November 2014

We study a noncommutative theory of gravity in the framework of torsional spacetime. This theory is based on a Lagrangian obtained by applying the technique of dimensional reduction of noncommutative gauge theory and that the yielded diffeomorphism invariant field theory can be made equivalent to a teleparallel formulation of gravity. Field equations are derived in the framework of teleparallel gravity through Weitzenbock geometry. We solve these field equations by considering a mass that is distributed spherically symmetrically in a stationary static spacetime in order to obtain a noncommutative line element. This new line element interestingly reaffirms the coherent state theory for a noncommutative Schwarzschild black hole. For the first time, we derive the Newtonian gravitational force equation in the commutative relativity framework, and this result could provide the possibility to investigate examples in various topics in quantum and ordinary theories of gravity.
Epithelial Antimicrobial Peptides: Guardian of the Oral Cavity
Authors: Hans Mayank , Madaan Hans Veenu
Published: November 2014

Gingival epithelium provides first line of defence from the microorganisms present in dental plaque. It not only provides a mechanical barrier but also has an active immune function too. Gingival epithelial cells participate in innate immunity by producing a range of antimicrobial peptides to protect the host against oral pathogens. These epithelial antimicrobial peptides (EAPs) include theβ-defensin family, cathelicidin (LL-37), calprotectin, and adrenomedullin. While some are constitutively expressed in gingival epithelial cells, others are induced upon exposure to microbial insults. It is likely that these EAPs have a role in determining the initiation and progression of oral diseases. EAPs are broad spectrum antimicrobials with a different but overlapping range of activity. Apart from antimicrobial activity, they participate in several other crucial roles in host tissues. Some of these, for instance,β-defensins, are chemotactic to immune cells. Others, such as calprotectin are important for wound healing and cell proliferation. Adrenomedullin, a multifunctional peptide, has its biological action in a wide range of tissues. Not only is it a potent vasodilator but also it has several endocrine effects. Knowing in detail the various bioactions of these EAPs may provide us with useful information regarding their utility as therapeutic agents.
Peritoneal Reactivity Evaluation in Horses Subjected to Experimental Small Colon Enterotomy and Treated with Subcutaneous Heparin
Authors: Alonso Juliana de Moura , Rodrigues Karoline Alves , Yamada Ana Lúcia Miluzzi , Watanabe Marcos Jun , Alves Ana Liz Garcia , Rodrigues Celso Antonio , Hussni Carlos Alberto
Published: November 2014

Heparin is routinely administered in postoperative abdominal surgery aiming to prevent adhesions formation; however, there is no consensus indicating its effectiveness. This study evaluated the effect of heparin on peritoneal reactivity after abdominal surgery, through the association between peritoneal fluid features and ultrasonographic and laparoscopic examination. Ten adult horses were used: control group (CG) and treated group (TG). Both groups underwent laparotomy and small colon enterotomy. TG received subcutaneous heparin at 150 IU/kg every 12 hours for 5 days. The animals underwent ultrasonography and peritoneal fluid examination prior to enterotomy (M0) 12 hours (M1), 1 day (M2), 2 days (M3), 4 days (M4), 6 days (M5), 10 days (M6), and 14 days after enterotomy (M7) with laparoscopic examination being performed on the fifth postoperative day. Peritoneal inflammatory response was observed in both groups. The peritoneal fluid of TG animals showed higher echogenicity during heparin therapy. No inflammatory difference was observed between groups through peritoneal fluid features, except for the higher D-dimer concentration in CG. On laparoscopy, slightly diffuse peritoneal reactivity for both groups was observed, being higher for TG. Laparoscopy and ultrasonography association allowed detailed access to the abdominal cavity. Ultrasonography assessed the diffuse peritoneal inflammation, and laparoscopy allowed the detailed analysis of the segments. No gross beneficial reactions resulting from the use of heparin on peritoneal reactivity were observed; however, it was observed by D-dimer evaluation that the TG had less fibrin deposition, which is directly related to a lower rate of abdominal adhesions formation.
Roles of 5-Lipoxygenase and Cysteinyl-Leukotriene Type 1 Receptors in the Hematological Response to Allergen Challenge and Its Prevention by Diethylcarbamazine in a Murine Model of Asthma
Authors: Masid-de-Brito Daniela , Queto Túlio , Gaspar-Elsas Maria Ignez C. , Xavier-Elsas Pedro
Published: November 2014

Diethylcarbamazine (DEC), which blocks leukotriene production, abolishes the challenge-induced increase in eosinopoiesis in bone-marrow from ovalbumin- (OVA-) sensitized mice, suggesting that 5-lipoxygenase (5-LO) products contribute to the hematological responses in experimental asthma models. We explored the relationship between 5-LO, central and peripheral eosinophilia, and effectiveness of DEC, using PAS or BALB/c mice and 5-LO-deficient mutants. We quantified eosinophil numbers in freshly harvested or cultured bone-marrow, peritoneal lavage fluid, and spleen, with or without administration of leukotriene generation inhibitors (DEC and MK886) and cisteinyl-leukotriene type I receptor antagonist (montelukast). The increase in eosinophil numbers in bone-marrow, observed in sensitized/challenged wild-type mice, was abolished by MK886 and DEC pretreatment. In ALOX mutants, by contrast, there was no increase in bone-marrow eosinophil counts, nor in eosinophil production in culture, in response to sensitization/challenge. In sensitized/challenged ALOX mice, challenge-induced migration of eosinophils to the peritoneal cavity was significantly reduced relative to the wild-type PAS controls. DEC was ineffective in ALOX mice, as expected from a mechanism of action dependent on 5-LO. In BALB/c mice, challenge significantly increased spleen eosinophil numbers and DEC treatment prevented this increase. Overall, 5-LO appears as indispensable to the systemic hematological response to allergen challenge, as well as to the effectiveness of DEC.
An Experimental Study on Strength and Durability for Utilization of Fly Ash in Concrete Mix
Authors: Karaşin Abdulhalim , Doğruyol Murat
Published: November 2014

The intention of this study is to discuss the variation of concrete exposed to high sulfate environment of a specific region with respect to strength and durability. Secondly, it is aimed to discuss the possibility of reducing the cement amount in construction of concrete structures. For this purpose, laboratory tests were conducted to investigate compressive strength and sulfate resisting capacity of concrete by using 20% fly ash as mineral additives, waste materials, instead of cement. As a case study the soil samples, received from Siirt Province areas which contain high sulfate rate, have been compared with respect to sulfate standard parameters of TS 12457-4. In such regions contact of underground water seep into hardened concrete substructures poses a risk of concrete deterioration. In order to determine the variation of strength and durability for concrete exposed to such aggressive environment, the samples were rested in a solution of Na2SO4(150 g/lt) in accordance with ASTM C 1012 for the tests. As a result of this experimental study, it is noted that the use of 20% fly ash, replacement material instead of cement, has no significant effect on compressive strength of concrete over time.
Expression of Genes Related to Prostaglandin Synthesis or Signaling in Human Subcutaneous and Omental Adipose Tissue: Depot Differences and Modulation by Adipogenesis
Authors: Michaud Andréanne , Lacroix-Pépin Nicolas , Pelletier Mélissa , Daris Marleen , Biertho Laurent , Fortier Michel A. , Tchernof André
Published: November 2014

Objectives. (1) To examine depot-specific PGE2and PGF2αrelease and mRNA expression of enzymes or receptors involved in PG synthesis or signaling in human adipose tissues; (2) to identify changes in expression of these transcripts through preadipocyte differentiation; and (3) to examine associations between adipose tissue mRNA expression of these transcripts and adiposity measurements.Methods. Fat samples were obtained surgically in women. PGE2and PGF2αrelease by preadipocytes and adipose tissue explants was measured. Expression levels of mRNA coding for enzymes or receptors involved in PG synthesis or signaling were measured by RT-PCR.Results. Cultured preadipocytes and explants from omental fat released more PGE2and PGF2αthan those from the subcutaneous depot and the corresponding transcripts showed consistent depot differences. Following preadipocyte differentiation, expression of PLA2G16 and PTGER3 mRNA was significantly increased whereas COX-1, COX-2, PTGIS, and PTGES mRNA abundance were decreased in both compartments (P≤0.01for all). Transcripts that were stimulated during adipogenesis were those that correlated best with adiposity measurements.Conclusion. Cells from the omental fat compartment release more PGE2and PGF2αthan those from the subcutaneous depot. Obesity modulates expression of PG-synthesizing enzymes and PG receptors which likely occurs through adipogenesis-induced changes in expression of these transcripts.
The Molecular Mechanism of Rhein in Diabetic Nephropathy
Authors: Zeng Cong-Cong , Liu Xi , Chen Guo-Rong , Wu Qian-Jia , Liu Wang-Wang , Luo Hai-Ying , Cheng Jin-Guo
Published: November 2014

Diabetic nephropathy (DN) is characterized by unclear pathogenesis. Recent medical data shows that the incidence of DN rises year by year. Rhein is the main compositions of rhubarb, a traditional Chinese medicinal plant, which plays an active role in kidney protection. The prophylaxis and phytotherapeutic effects of rhein are due to its anti‐inflammatory and antifibrosis properties. Here, we shed light on the renal protective role of rhein in diabetes mellitus (DM) with a particular focus on the molecular basis of this effect.
Xylazine as a Drug of Abuse and Its Effects on the Generation of Reactive Species and DNA Damage on Human Umbilical Vein Endothelial Cells
Authors: Silva-Torres Luz , Veléz Christian , Álvarez Lyvia , Zayas Beatriz
Published: November 2014

Human xylazine (XYL) abuse among addicts has received great interest due to its potential toxic effects upon addicts and the need to understand the mechanism of action associated with the potential health effects. XYL is an alpha-2 agonist restricted to veterinarian applications, without human medical applications. Our previous work demonstrated that XYL and its combination with cocaine (COC) and/or 6-monoacetylmorphine (6-MAM) induce cell death through an apoptotic mechanism. The aim of this study was to determine the effect of xylazine on the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as well as DNA damage on endothelial cell. Human umbilical vein endothelial cells (HUVEC) were treated with XYL (60 μM), COC (160 μM), 6-MAM (160 μM), camptothecin (positive control, 50 μM), XYL/COC (50 μM), XYL/6-MAM (50 μM), and XYL/COC/6-MAM (40 μM) for a period of 24 hours. Generation of intracellular ROS, RNS, and DNA fragmentation were analyzed using a fluorometric assay. Results reveal that XYL and 6-MAM increase levels of ROS; no induction of RNS production was observed. The combination of these drugs shows significant increase in DNA fragmentation in G2/M phase, while XYL, COC, and 6-MAM, without combination, present higher DNA fragmentation in G0/G1 phase. These findings support that these drugs and their combination alter important biochemical events aligned with an apoptotic mechanism of action in HUVEC.
Effectiveness and Safety of Infliximab in Two Cases of Severe Chondrocalcinosis: Nine Years of Follow-Up
Authors: Bruges-Armas Jácome , Bettencourt Bruno F. , Couto Ana R. , Lima Manuela , Rodrigues Ana M. , Vastesaeger Nathan , Brown Matthew A.
Published: November 2014

Objectives. To investigate the efficacy of infliximab in the treatment of severe calcium pyrophosphate deposition diseases (CPPD).Methods. Two patients with severe CPPD and diffuse idiopathic skeletal hyperostosis- (DISH-) like phenotype are described. Both patients were resistant to therapy with nonsteroidal anti-inflammatory drugs (NSAIDs). Both patients were treated with infliximab, a TNF-αreceptor antagonist, for nine years.Results. Treatment with infliximab resulted in major clinical and laboratory improvements without relevant side effects.Conclusions. These results suggest that infliximab may be an effective treatment of severe CPDD.
Occurrence of Enterobacteriaceae in Raw Meat and in Human Samples from Egyptian Retail Sellers
Authors: Gwida Mayada , Hotzel Helmut , Geue Lutz , Tomaso Herbert
Published: November 2014

The present study was performed to assess the presence of Enterobacteriaceae in raw meat and handlers in Egypt using cultivation and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 100 raw meat samples (chicken and beef meat, 50 each) were randomly purchased from butchers and local meat retailers located at Mansoura city, Egypt. Fifty human samples were collected from meat handlers (hand swabs and stool specimens, 25 each). 228 bacterial isolates were recovered from these samples. Unidentified isolates were characterized by partial 16S rRNA gene sequencing. Escherichia coli isolates were further typed using a DNA microarray system. Proteus spp. (60.0%) were found to be the most abundant followed by Escherichia coli (38.7%), Klebsiella spp. (17.3%), and Citrobacter spp. (13.3%). The presence of different Enterobacteriaceae in locally produced retail raw meat demonstrates the risk of infection of people through consumption of raw or undercooked meat and the risk for cross-contamination of other food products. Harmonized and concerted actions from veterinary and public health authorities are needed to reduce the risk of infection.
Inherent Difference in Saliency for Generators with Different PM Materials
Authors: Eriksson Sandra
Published: November 2014

The inherent differences between salient and nonsalient electrical machines are evaluated for two permanent magnet generators with different configurations. The neodymium based (NdFeB) permanent magnets (PMs) in a generator are substituted with ferrite magnets and the characteristics of the NdFeB generator and the ferrite generator are compared through FEM simulations. The NdFeB generator is a nonsalient generator, whereas the ferrite machine is a salient-pole generator, with small saliency. The two generators have almost identical properties at rated load operation. However, at overload the behaviour differs between the two generators. The salient-pole, ferrite generator has lower maximum torque than the NdFeB generator and a larger voltage drop at high current. It is concluded that, for applications where overload capability is important, saliency must be considered and the generator design adapted according to the behaviour at overload operation. Furthermore, if the maximum torque is the design criteria, additional PM mass will be required for the salient-pole machine.
Responses of Carbon Dynamics to Nitrogen Deposition in Typical Freshwater Wetland of Sanjiang Plain
Authors: Wang Yang , Liu Jingshuang , He Longxue , Dou Jingxin , Zhao Hongmei
Published: November 2014

The effects of nitrogen deposition (N-deposition) on the carbon dynamics in typicalCalamagrostis angustifoliawetland of Sanjiang Plain were studied by a pot-culture experiment during two continuous plant growing seasons. Elevated atmospheric N-deposition caused significant increases in the aboveground net primary production and root biomass; moreover, a preferential partition of carbon to root was also observed. Different soil carbon fractions gained due to elevated N-deposition and their response intensities followed the sequence of labile carbon > dissolved organic carbon > microbial biomass carbon, and the interaction between N-deposition and flooded condition facilitated the release of different carbon fractions. Positive correlations were found between CO2and CH4fluxes and liable carbon contents with N-deposition, and flooded condition also tended to facilitate CH4fluxes and to inhibit the CO2fluxes with N-deposition. The increases in soil carbon fractions occurring in the nitrogen treatments were significantly correlated with increases in root, aboveground parts, total biomass, and their carbon uptake. Our results suggested that N-deposition could enhance the contents of active carbon fractions in soil system and carbon accumulation in plant of the freshwater wetlands.
Modeling Slump of Ready Mix Concrete Using Genetically Evolved Artificial Neural Networks
Authors: Chandwani Vinay , Agrawal Vinay , Nagar Ravindra
Published: November 2014

Artificial neural networks (ANNs) have been the preferred choice for modeling the complex and nonlinear material behavior where conventional mathematical approaches do not yield the desired accuracy and predictability. Despite their popularity as a universal function approximator and wide range of applications, no specific rules for deciding the architecture of neural networks catering to a specific modeling task have been formulated. The research paper presents a methodology for automated design of neural network architecture, replacing the conventional trial and error technique of finding the optimal neural network. The genetic algorithms (GA) stochastic search has been harnessed for evolving the optimum number of hidden layer neurons, transfer function, learning rate, and momentum coefficient for backpropagation ANN. The methodology has been applied for modeling slump of ready mix concrete based on its design mix constituents, namely, cement, fly ash, sand, coarse aggregates, admixture, and water-binder ratio. Six different statistical performance measures have been used for evaluating the performance of the trained neural networks. The study showed that, in comparison to conventional trial and error technique of deciding the neural network architecture and training parameters, the neural network architecture evolved through GA was of reduced complexity and provided better prediction performance.
Parathyroid Gland Involvement by Thyroid Cancer: Results from a Large Series of Thyroidectomies Performed in Two Italian University Hospitals and Review of the Literature
Authors: Papi Giampaolo , Corrado Stefania , Fadda Guido , Maiorana Antonino , Maccio Livia , Corsello Salvatore Maria , Pontecorvi Alfredo
Published: November 2014

Objectives.Parathyroid involvement by thyroid cancer (TC) has not been frequently investigated in thyroidectomy-based studies. We aimed to detect cases of parathyroid invasion by TC in a large series of thyroidectomies and to review the literature on this topic.Study Design.A 10-yr period database research was made from the files of the Section of Pathology of two Italian University Hospitals. Out of 22,310 thyroidectomies, 10 patients with parathyroid involvement by TC were found.Results.The 10 patients, 7 females and 3 males, aged55±14years (range 34–76, median 56) had papillary thyroid carcinoma and accounted for 0.4% of subjects affected by all TCs and submitted to thyroidectomy. The tumor invaded perithyroid soft tissues in 6 patients and central neck (level VI) lymph nodes in 3. Parathyroid involvement by TC occurred by infiltration in 6 cases, extension through an intervening pseudocapsule in 1, and both patterns in 3. All patients are alive and disease free at5.6±3-yr follow-up.Conclusion.Limited to thyroidectomy series, our results and literature data suggest that parathyroid involvement by TC has a 0.4–3.9% incidence rate; mainly affects women in their sixth-seventh decade of life; is associated to a good prognosis, unless massive extrathyroid extension of TC occurs.
A Modifiedo-Phthalaldehyde Fluorometric Analytical Method for Ultratrace Ammonium in Natural Waters Using EDTA-NaOH as Buffer
Authors: Hu Hongzhi , Liang Ying , Li Shuo , Guo Qing , Wu Chancui
Published: November 2014

In the existence of appropriate amount of disodium ethylenediaminetetraacetate (EDTA), precipitation would not occur in seawater and other natural waters even if the sample solution was adjusted to strong basicity, and the NH3-OPA-sulfite reaction at the optimal pH range could be used to determine ammonium in natural waters. Based on this, a modifiedo-phthalaldehyde fluorometric analytical method has been established to determine ultratrace ammonium in natural waters. Experimental parameters, including reagent concentration, pH, reaction time, and effect of EDTA, were optimized throughout the experiments based on univariate experimental design. The results showed that the optimal pH range was between 10.80 and 11.70. EDTA did not obviously affect the fluorometric intensity. The linearity range of the proposed method was 0.032–0.500 µmol/L, 0.250–3.00 µmol/L, and 1.00–20.0 µmol/L at the excitation/emission slit of 3 nm/5 nm, 3 nm/3 nm, and 1.5 nm/1.5 nm, respectively. The method detection limit was 0.0099 µmol/L. Compared to the classical OPA method, the proposed method had the advantage of being more sensitive and could quantify ultratrace ammonium without enrichment.
Seasonal/Interannual Variations of Carbon Sequestration and Carbon Emission in a Warm-Season Perennial Grassland
Authors: Dhital Deepa , Inoue Tomoharu , Koizumi Hiroshi
Published: November 2014

Carbon sequestration and carbon emission are processes of ecosystem carbon cycling that can be affected while land area converted to grassland resulting in increased soil carbon storage and below-ground respiration. Discerning the importance of carbon cycle in grassland, we aimed to estimate carbon sequestration in photosynthesis and carbon emission in respiration from soil, root, and microbes, for four consecutive years (2007–2010) in a warm-season perennial grassland, Japan. Soil carbon emission increased with increasing growing season temperature which ranged from 438 to 1642 mg CO2 m−2 h−1. Four years’ average soil carbon emission for growing season, nongrowing season, and annual emission was 1123, 364, and 1488 g C m−2, respectively. Nongrowing and snow covered season soil carbon emission contributed 23–25% and 14–17% to the annual emission. Above-ground biomass varied seasonally and variation in green biomass affected soil carbon emission with increasing temperature and precipitation. Temperature effect on root carbon emission contributed about 1/4th of the total soil carbon emission. Variation in soil and root carbon emission is affected by below-ground biomass. Long-term estimation concluded that seasonal and interannual variations in carbon sequestration and emission are very common in grassland ecosystem.