bluuebunny/crossref_metadata_2025 huggingface dataset:
Facile Synthesis of Surface-Modified Hollow-Silica (SiO2) Aerogel Particles via Oil–Water–Oil Double Emulsion Method
Authors: Kapadnis Pratik S. , Nam Ki-Sun , Kim Hyun-Young , Park Hyung-Ho , Hwang Haejin
Published: June 2024

Due to their high surface area and low weight, silica aerogels are ideally suited for several uses, including drug delivery, catalysis, and insulation. Oil–water–oil (OWO) double emulsion is a simple and regulated technique for encasing a volatile oil phase in a silica shell to produce hollow silica (SiO2) aerogel particles by using hydrophilic and hydrophobic emulsifiers. In this study, the oil–water–oil (OWO) double emulsion method was implemented to synthesize surface-modified hollow silica (SiO2) aerogel particles in a facile and effective way. This investigation mainly focused on the influence of the N-hexane-to-water glass (OW) ratio (r) in the first emulsion, silica (water glass) content concentration (x), and surfactant concentration (s) variations. Furthermore, surface modification techniques were utilized to customize the aerogel’s characteristics. The X-ray diffraction (XRD) patterns showed no imprints of impurities except SiO2. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images highlight the hollow microstructure of silica particles. Zeta potential was used to determine particle size analysis of hollow silica aerogel particles. The oil–water–oil (OWO) double emulsion approach was successfully employed to synthesize surface-modified hollow silica (SiO2) aerogel particles, providing precise control over the particle characteristics. By the influence of the optimization condition, this approach improves the aerogel’s potential applications in drug delivery, catalysis, and insulation by enabling surface modifications.
“Wartime” Ephemera from the Family Home in German and Austrian History Museums: A Counterexample to the British Case
Authors: Paver Chloe
Published: June 2024

When discussing “wartime ephemera”, of the kind that has been passed down through families since the Second World War, Germany and Austria could be considered as a counterexample to Britain. In German and Austrian historical memory, “wartime” cannot be separated from pre-war Nazi society (beginning in 1933 and 1938, respectively). Moreover, what we might loosely call the “Antiques Roadshow experience”—discussing family objects from the Second World War in a sympathetic public forum—has never been open to the majority of Germans and Austrians, who were rather inclined to hide and forget such objects in the family home. Even so, mundane Nazi-era objects survived in their millions and this essay discusses their display in German and Austrian history museums. There, they serve to illustrate a history of mentalities during and after the Nazi regime. Austrian museums are currently playing a proactive role in the transfer of objects from the private, family archive to public, cultural memory. This article considers how notions of “family” are constructed in museum discourse and asks how the millions of German and Austrian citizens and residents with no family connection to the Second World War can be included in a form of national family storytelling that arguably “re-ethnicizes” memory.
Critical Adoptee Standpoint: Transnational, Transracial Adoptees as Knowledge Producers
Authors: Laybourn SunAh Marie
Published: June 2024

Drawing on Asian adoptee-authored research, this article conceptualizes a critical adoptee standpoint. It underscores the significance of adoptees as knowledge producers and offers new insights into family dynamics, racialization processes, and adoptee personhood. Through three conceptual themes derived from adoptee-authored research, it illuminates the intersectional power dynamics shaping adoptees’ lived experiences and challenges traditional adoption narratives. This approach repositions adoptees as agentic subjects who have cultivated a group consciousness that transcends traditional boundaries of belonging. While focused on Asian adoptees, the essay ultimately calls for broader recognition of adoptees’ contributions to adoption discourse and a more comprehensive understanding of a critical adoptee standpoint in both academic and advocacy settings and among the broader adoptee population.
PON1, APOE and SDF-1 Gene Polymorphisms and Risk of Retinal Vein Occlusion: A Case-Control Study
Authors: Ragkousis Antonios , Kazantzis Dimitrios , Georgalas Ilias , Theodossiadis Panagiotis , Kroupis Christos , Chatziralli Irini
Published: June 2024

Numerous studies have tried to evaluate the potential role of thrombophilia-related genes in retinal vein occlusion (RVO); however, there is limited research on genes related to different pathophysiological mechanisms involved in RVO. In view of the strong contribution of oxidative stress and inflammation to the pathogenesis of RVO, the purpose of the present study was to investigate the association of inflammation- and oxidative-stress-related polymorphisms from three different genes [apolipoprotein E (APOE), paraoxonase 1 (PON1) and stromal cell-derived factor 1 (SDF-1)] and the risk of RVO in a Greek population. Participants in this case-control study were 50 RVO patients (RVO group) and 50 healthy volunteers (control group). Blood samples were collected on EDTA tubes and genomic DNA was extracted. Genotyping of rs854560 (L55M) and rs662 (Q192R) for the PON1 gene, rs429358 and rs7412 for the APOE gene and rs1801157 [SDF1-3′G(801)A] for SDF-1 gene was performed using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. Multiple genetic models (codominant, dominant, recessive, overdominant and log-additive) and haplotype analyses were performed using the SNPStats web tool to assess the correlation between the genetic polymorphisms and the risk of RVO. Binary logistic regression analysis was used for the association analysis between APOE gene variants and RVO. Given the multifactorial nature of the disease, our statistical analysis was adjusted for the most important systemic risk factors (age, hypertension and diabetes mellitus). The dominant genetic model for the PON1 Q192R single nucleotide polymorphism (SNP) of the association analysis revealed that there was a statistically significant difference between the RVO group and the control group. Specifically, after adjusting for age and hypertension, the PON1 192 R allele (QR + RR) was found to be associated with a statistically significantly higher risk of RVO compared to the QQ genotype (OR = 2.51; 95% CI = 1.02–6.14, p = 0.04). The statistically significant results were maintained after including diabetes in the multivariate model in addition to age and hypertension (OR = 2.83; 95% CI = 1.01–7.97, p = 0.042). No statistically significant association was revealed between the other studied polymorphisms and the risk of RVO. Haplotype analysis for PON1 SNPs, L55M and Q192R, revealed no statistically significant correlation. In conclusion, PON1 192 R allele carriers (QR + RR) were associated with a statistically significantly increased risk of RVO compared to the QQ homozygotes. These findings suggest that the R allele of the PON1 Q192R is likely to play a role as a risk factor for retinal vein occlusion.
Gene Therapy for Non-Hereditary Retinal Disease: Age-Related Macular Degeneration, Diabetic Retinopathy, and Beyond
Authors: Rowe Lucas , Ciulla Thomas
Published: June 2024

Gene therapy holds promise as a transformative approach in the treatment landscape of age-related macular degeneration (AMD), diabetic retinopathy (DR), and diabetic macular edema (DME), aiming to address the challenges of frequent intravitreal anti-vascular endothelial growth factor (VEGF) injections. This manuscript reviews ongoing gene therapy clinical trials for these disorders, including ABBV-RGX-314, ixoberogene soroparvovec (ixo-vec), and 4D-150. ABBV-RGX-314 utilizes an adeno-associated virus (AAV) vector to deliver a transgene encoding a ranibizumab-like anti-VEGF antibody fragment, demonstrating promising results in Phase 1/2a and ongoing Phase 2b/3 trials. Ixo-vec employs an AAV2.7m8 capsid for intravitreal delivery of a transgene expressing aflibercept, showing encouraging outcomes in Phase 1 and ongoing Phase 2 trials. 4D-150 utilizes an evolved vector to express both aflibercept and a VEGF-C inhibitory RNAi, exhibiting positive interim results in Phase 1/2 studies. Other therapies reviewed include EXG102-031, FT-003, KH631, OLX10212, JNJ-1887, 4D-175, and OCU410. These therapies offer potential advantages of reduced treatment frequency and enhanced safety profiles, representing a paradigm shift in management towards durable and efficacious cellular-based biofactories. These advancements in gene therapy hold promise for improving outcomes in AMD and addressing the complex challenges of DME and DR, providing new avenues for the treatment of diabetic eye diseases.
Knockout of the Chlorophyll a Oxygenase Gene OsCAO1 Reduces Chilling Tolerance in Rice Seedlings
Authors: Xiong Jiayi , Wen Genping , Song Jin , Liu Xiaoyi , Chen Qiuhong , Zhang Guilian , Xiao Yunhua , Liu Xiong , Deng Huabing , Tang Wenbang , Wang Feng , Lu Xuedan
Published: June 2024

Chilling stress is one of the main abiotic factors affecting rice growth and yield. In rice, chlorophyllide a oxygenase encoded by OsCAO1 is responsible for converting chlorophyllide a to chlorophyllide b, playing a crucial role in photosynthesis and thus rice growth. However, little is known about the function of OsCAO1 in chilling stress responses. The presence of the cis-acting element involved in low-temperature responsiveness (LTR) in the OsCAO1 promoter implied that OsCAO1 probably is a cold-responsive gene. The gene expression level of OsCAO1 was usually inhibited by low temperatures during the day and promoted by low temperatures at night. The OsCAO1 knockout mutants generated by the CRISPR-Cas9 technology in rice (Oryza sativa L.) exhibited significantly weakened chilling tolerance at the seedling stage. OsCAO1 dysfunction led to the accumulation of reactive oxygen species and malondialdehyde, an increase in relative electrolyte leakage, and a reduction in antioxidant gene expression under chilling stress. In addition, the functional deficiency of OsCAO1 resulted in more severe damage to chloroplast morphology, such as abnormal grana thylakoid stacking, caused by low temperatures. Moreover, the rice yield was reduced in OsCAO1 knockout mutants. Therefore, the elevated expression of OsCAO1 probably has the potential to increase both rice yield and chilling tolerance simultaneously, providing a strategy to cultivate chilling-tolerant rice varieties with high yields.
Comparative Analysis of Shapley Values Enhances Transcriptomics Insights across Some Common Uterine Pathologies
Authors: Castro-Martínez José A. , Vargas Eva , Díaz-Beltrán Leticia , Esteban Francisco J.
Published: June 2024

Uterine pathologies pose a challenge to women’s health on a global scale. Despite extensive research, the causes and origin of some of these common disorders are not well defined yet. This study presents a comprehensive analysis of transcriptome data from diverse datasets encompassing relevant uterine pathologies such as endometriosis, endometrial cancer and uterine leiomyomas. Leveraging the Comparative Analysis of Shapley values (CASh) technique, we demonstrate its efficacy in improving the outcomes of the classical differential expression analysis on transcriptomic data derived from microarray experiments. CASh integrates the microarray game algorithm with Bootstrap resampling, offering a robust statistical framework to mitigate the impact of potential outliers in the expression data. Our findings unveil novel insights into the molecular signatures underlying these gynecological disorders, highlighting CASh as a valuable tool for enhancing the precision of transcriptomics analyses in complex biological contexts. This research contributes to a deeper understanding of gene expression patterns and potential biomarkers associated with these pathologies, offering implications for future diagnostic and therapeutic strategies.
Molecular Mechanisms Governing Sight Loss in Inherited Cone Disorders
Authors: Brotherton Chloe , Megaw Roly
Published: June 2024

Inherited cone disorders (ICDs) are a heterogeneous sub-group of inherited retinal disorders (IRDs), the leading cause of sight loss in children and working-age adults. ICDs result from the dysfunction of the cone photoreceptors in the macula and manifest as the loss of colour vision and reduced visual acuity. Currently, 37 genes are associated with varying forms of ICD; however, almost half of all patients receive no molecular diagnosis. This review will discuss the known ICD genes, their molecular function, and the diseases they cause, with a focus on the most common forms of ICDs, including achromatopsia, progressive cone dystrophies (CODs), and cone–rod dystrophies (CORDs). It will discuss the gene-specific therapies that have emerged in recent years in order to treat patients with some of the more common ICDs.
Construction of a Full-Length Transcriptome of Western Honeybee Midgut Tissue and Improved Genome Annotation
Authors: Zang He , Guo Sijia , Dong Shunan , Song Yuxuan , Li Kunze , Fan Xiaoxue , Qiu Jianfeng , Zheng Yidi , Jiang Haibin , Wu Ying , Lü Yang , Chen Dafu , Guo Rui
Published: June 2024

Honeybees are an indispensable pollinator in nature with pivotal ecological, economic, and scientific value. However, a full-length transcriptome for Apis mellifera, assembled with the advanced third-generation nanopore sequencing technology, has yet to be reported. Here, nanopore sequencing of the midgut tissues of uninoculated and Nosema ceranae-inoculated A. mellifera workers was conducted, and the full-length transcriptome was then constructed and annotated based on high-quality long reads. Next followed improvement of sequences and annotations of the current reference genome of A. mellifera. A total of 5,942,745 and 6,664,923 raw reads were produced from midguts of workers at 7 days post-inoculation (dpi) with N. ceranae and 10 dpi, while 7,100,161 and 6,506,665 raw reads were generated from the midguts of corresponding uninoculated workers. After strict quality control, 6,928,170, 6,353,066, 5,745,048, and 6,416,987 clean reads were obtained, with a length distribution ranging from 1 kb to 10 kb. Additionally, 16,824, 17,708, 15,744, and 18,246 full-length transcripts were respectively detected, including 28,019 nonredundant ones. Among these, 43,666, 30,945, 41,771, 26,442, and 24,532 full-length transcripts could be annotated to the Nr, KOG, eggNOG, GO, and KEGG databases, respectively. Additionally, 501 novel genes (20,326 novel transcripts) were identified for the first time, among which 401 (20,255), 193 (13,365), 414 (19,186), 228 (12,093), and 202 (11,703) were respectively annotated to each of the aforementioned five databases. The expression and sequences of three randomly selected novel transcripts were confirmed by RT-PCR and Sanger sequencing. The 5′ UTR of 2082 genes, the 3′ UTR of 2029 genes, and both the 5′ and 3′ UTRs of 730 genes were extended. Moreover, 17,345 SSRs, 14,789 complete ORFs, 1224 long non-coding RNAs (lncRNAs), and 650 transcription factors (TFs) from 37 families were detected. Findings from this work not only refine the annotation of the A. mellifera reference genome, but also provide a valuable resource and basis for relevant molecular and -omics studies.
Transcriptional Comparison Reveals Differential Resistance Mechanisms between CMV-Resistant PBC688 and CMV-Susceptible G29
Authors: Guo Guangjun , Pan Baogui , Gong Chengsheng , Wang Shubin , Liu Jinbing , Gao Changzhou , Diao Weiping
Published: June 2024

The Cucumber mosaic virus (CMV) presents a significant threat to pepper cultivation worldwide, leading to substantial yield losses. We conducted a transcriptional comparative study between CMV-resistant (PBC688) and -susceptible (G29) pepper accessions to understand the mechanisms of CMV resistance. PBC688 effectively suppressed CMV proliferation and spread, while G29 exhibited higher viral accumulation. A transcriptome analysis revealed substantial differences in gene expressions between the two genotypes, particularly in pathways related to plant–pathogen interactions, MAP kinase, ribosomes, and photosynthesis. In G29, the resistance to CMV involved key genes associated with calcium-binding proteins, pathogenesis-related proteins, and disease resistance. However, in PBC688, the crucial genes contributing to CMV resistance were ribosomal and chlorophyll a–b binding proteins. Hormone signal transduction pathways, such as ethylene (ET) and abscisic acid (ABA), displayed distinct expression patterns, suggesting that CMV resistance in peppers is associated with ET and ABA. These findings deepen our understanding of CMV resistance in peppers, facilitating future research and variety improvement.
Photogrammetry from UAV and Low-Cost Lidar for Sinkhole Hazard Mitigation in Urban Areas: Applications and Evaluations
Authors: Gentili Francesco , Madonna Sergio
Published: June 2024

The Italian national territory is characterised by the widespread presence of cavities dating back to different periods, especially in urban areas. The lack of knowledge of the position of the entrances, planimetric developments and state of preservation contributes to accentuating the unknowns related to sinkhole risk, which are directly related to potential cavity collapses with the opening of surface chasms. To deepen knowledge with a view to risk mitigation, a method has been developed to employ surveys obtained from Unmanned Aerial Vehicles (UAVs) to locate entrances even in hard-to-access urban areas. These surveys, properly supported with GNSS stations, were then integrated with cavity surveys obtained from low-cost lidar mounted on iPhones. Comparisons were made with traditional surveying techniques to better understand the reliability of the surveys made with low-cost lidar. The 3D models obtained, combined with geomechanical surveys of the rock masses hosting the cavities, allowed the application of simplified and empirical methods for an initial stability assessment. This method was tested on a portion of the municipality of Grotte di Castro (Province of Viterbo—Italy).
Modelling and Validation of the Derna Dam Break Event
Authors: Annunziato Alessandro , Santini Marzia , Proietti Chiara , de Girolamo Ludovica , Lorini Valerio , Gerhardinger Andrea , Tucci Michele
Published: June 2024

The catastrophic failure of two dams in Libya on 10 and 11 September 2023 resulted in the devastating flooding of the city of Derna, which is located downstream of the dams, causing more than 6000 fatalities and displacing thousands of residents. The failure was attributed to heavy rainfall from Storm Daniel, leading to the dams reaching full capacity and subsequently overflowing and failing. This paper presents an analysis of the dam break, including the modelling of flow discharge and the resulting flooding of Derna. For validation purposes, this study compares the modelled quantities with post-event satellite imagery from UNOSAT and Copernicus, local reports, and data collected from social media using AI detection. The findings provide valuable insights into the dynamics of the dam break and its initial parameters, as well as an assessment of the accuracy of the results. The analysis is performed using a rapid estimation technique developed by JRC to provide the international emergency community with a swift overview of the impact and damage assessment of potential or actual dam break events. The use of all available data shows a satisfactory comparison with the calculated quantities. The rapid modelling of dam break events and combined analysis of multiple data types are proven suitable for promptly assessing the expected dynamic of the event, as well as reconstructing the unknown initial conditions before the break. Incorporating sensitivity analyses provides an estimate of the uncertainties associated with the deduced values of the unknown parameters and their relative importance in the analysis.
Diaphragm Ultrasound in Different Clinical Scenarios: A Review with a Focus on Older Patients
Authors: Siniscalchi Carmine , Nouvenne Antonio , Cerundolo Nicoletta , Meschi Tiziana , Ticinesi Andrea ,   on behalf of the Parma Post-Graduate Specialization School in Emergency-Urgency Medicine Interest Group on Thoracic Ultrasound
Published: June 2024

Diaphragm muscle dysfunction is increasingly recognized as a fundamental marker of several age-related diseases and conditions including chronic obstructive pulmonary disease, heart failure and critical illness with respiratory failure. In older individuals with physical frailty and sarcopenia, the loss of muscle mass and function may also involve the diaphragm, contributing to respiratory dysfunction. Ultrasound has recently emerged as a feasible and reliable strategy to visualize diaphragm structure and function. In particular, it can help to predict the timing of extubation in patients undergoing mechanical ventilation in intensive care units (ICUs). Ultrasonographic evaluation of diaphragmatic function is relatively cheap, safe and quick and can provide useful information for real-time monitoring of respiratory function. In this review, we aim to present the current state of scientific evidence on the usefulness of ultrasound in the assessment of diaphragm dysfunction in different clinical settings, with a particular focus on older patients. We highlight the importance of the qualitative information gathered by ultrasound to assess the integrity, excursion, thickness and thickening of the diaphragm. The implementation of bedside diaphragm ultrasound could be useful for improving the quality and appropriateness of care, especially in older subjects with sarcopenia who experience acute respiratory failure, not only in the ICU setting.
SGLT2 Inhibitors and Uric Acid Homeostasis
Authors: Zapf Ava M. , Woodward Owen M.
Published: June 2024

A relationship between metabolic disorders and hyperuricemia is well established. The nature of the relationship—risk factor, causal agent, or byproduct—remains unclear. Recent studies of sodium–glucose transporter 2 inhibitors (SGLT2i’s) have established that this pharmacological intervention is beneficial to patients with hyperglycemia and type 2 diabetes mellitus (T2D) and also against the common cardio and renal comorbidities associated with diabetes. Hyperuricemia, or high plasma uric acid levels, is one of the comorbidities mitigated with SGLT2i treatment, raising the potential for using SGLT2i’s as part of the treatment for gout and hyperuricemia. However, the mechanisms underlying the lower plasma urate levels and increased uricosuria produced with SGLT2i’s remains poorly understood. Here, we review the renal physiology of glucose and uric acid transport, the renal consequences of hyperglycosuria and diabetes, the benefits and physiology of SGLT2i use, and discuss several potential mechanisms that may be responsible for the favorable uricosuric effect observed in those treated with SGLT2i’s.
Decolonial Embodiments: Materiality, Disability, and Black Being in Djaimilia Pereira de Almeida’s Luanda, Lisboa, Paraíso
Authors: Silva Daniel F.
Published: June 2024

Grounded in, and in dialogue with, Djaimilia Pereira de Almeida’s Luanda, Lisboa, Paraíso of 2018, this paper interrogates a particular time and place of coloniality and racial capital’s reproduction of Black fungibility in late twentieth-century Portugal, after formal decolonization in Africa and in the wake of Black migratory waves from the post/neo-colony (Angola in this case) to the former metropolis. Almeida’s novel provides a literary intervention in grappling with the economic and institutional reinvention of anti-Blackness in Europe after settler colonialism, while also imagining and inscribing modes of Black being within and beyond the materialities of white supremacy. Towards this end and against the racial, gendered, and ableist logics of capital, the Black body in Almeida’s novel becomes a site through which the relationships between humans and matter as well as mind and body are decolonially revised.
Nursing Intervention to Prevent and Manage Delirium in Critically Ill Patients: A Scoping Review
Authors: Fernandes Filipa , Santos Mariana , Anacleto Ana Margarida , Jerónimo Cátia , Ferreira Óscar , Baixinho Cristina Lavareda
Published: June 2024

Delirium is an acute neuropsychiatric syndrome of multifactorial etiology with a high incidence in people admitted to intensive care units. In addition to reversible impairment of cognitive processes, it may be associated with changes in thinking and perception. If, in the past, it was considered an expected complication of severe disease, nowadays, delirium is associated with a poor short-term and long-term prognosis. Knowing that its prevention and early identification can reduce morbidity, mortality, and health costs, it is vital to investigate nursing interventions focused on delirium in critically ill patients. This study aimed to identify nursing interventions in the prevention and management of delirium in critically ill adults. The method used to answer the research question was a scoping review. The literature search was performed in the Medline (via PubMed), CINAHL (via EBSCOhost), Scopus, Web of Science, and JBI databases. The final sample included 15 articles. Several categories of non-pharmacological interventions were identified, addressing the modifiable risk factors that contribute to the development of delirium, and for which nurses have a privileged position in their minimization. No drug agent can, by itself, prevent or treat delirium. However, psychoactive drugs are justified to control hyperactive behaviors through cautious use. Early diagnosis, prevention, or treatment can reduce symptoms and improve the individual’s quality of life. Therefore, nursing professionals must ensure harmonious coordination between non-pharmacological and pharmacological strategies.
An Overview of Opioid Prescription Patterns among Non-Opioid Users Following Emergency Department Admission
Authors: Zeino Miriam , Léguillon Romain , Brevet Pauline , Gerard Baptiste , Chenailler Catherine , Raymond Johanna , Bibaut Lucas , Pouplin Sophie , Joly Luc Marie , Varin Rémi , Barat Eric
Published: June 2024

The evolving landscape of opioid prescription practices necessitates a comprehensive understanding of emerging patterns, particularly among new opioid users discharged from emergency departments. This study delves into the intricate realm of opioid utilization by elucidating the prevalence of their prescriptions. A retrospective analysis of electronic health records was conducted, including a cohort of 71 patients who received opioid prescriptions upon discharge from emergency departments from 1 January 2022 to 30 June 2022. Demographic characteristics and prescription details were systematically examined. This study illuminates tramadol’s prominence, with 84% of prescriptions and a Defined Daily Dose (DDD) morphine equivalent of 60 mg, as the primary choice as a new opioid, a finding that draws attention due to the closely aligned dosages with morphine equivalents. This discovery prompts a critical reassessment of tramadol’s therapeutic role, considering its multifaceted nature encompassing serotonergic effects and heightened fall risks. This study advocates for a nuanced and vigilant approach to tramadol prescription, cognizant of its potential risks and therapeutic implications, and highlights the imperative of optimizing data quality and traceability within electronic health records to enhance patient care and facilitate future research endeavors.
Reflections on the Decay Mechanisms of Half-Timbered Walls in Traditional Spanish Architecture: Statistical Analysis of Material and Structural Damage
Authors: Hueto-Escobar Alicia , Vegas Fernando , Mileto Camilla , Lidón de Miguel María
Published: June 2024

Knowledge on the state of conservation and vulnerability of traditional techniques when faced with the most common degradation phenomena is vital in order to propose the most suitable conservation and maintenance actions. This article presents the systematic review of 1218 half-timbered walls found throughout Spain, enabling the identification of a total of 27 material lesions, classified by atmospheric, biological or anthropic origin, and 9 structural lesions due to stress or excessive deformation. Their qualitative and quantitative analysis has focused on the frequency of the individual lesions and the possible correlation with different constructive characteristics, such as the materials used, the geometry of the framework and the presence of plinths, eaves and protective rendering. Almost the entire sample presents some degree of material degradation, mostly atmospheric lesions of limited severity, such as superficial atmospheric erosion and chromatic alteration and dehydration of the timber. In terms of structural lesions, half-timbered walls are seen to be more vulnerable to this type of deformation. Considering the risk of loss affecting all traditional architecture, it becomes particularly important to promote the continued maintenance of half-timbered walls in order to reduce the influence of material lesions caused by atmospheric agents. Subsequently, suitable criteria for intervention are established in order to reduce the effect of anthropic lesions and structural degradation phenomena, particularly linked to a lack of maintenance and modifications of anthropic origin.
Do Climate Conditions Affect the Quality of the Apiaceae Fruits’ Essential Oils?
Authors: Lončar Biljana , Pezo Lato , Pezo Milada , Jovanović Aca , Šuput Danijela , Radosavljević Miloš , Aćimović Milica
Published: June 2024

This study investigated the impact of climate conditions on the quality of essential oils extracted from Apiaceae fruits, specifically coriander (Coriandrum sativum var. microcarpum), aniseed (Pimpinella anisum), and annual caraway (Carum carvi var. annuum) grown at three distinct locations in Serbia over three consecutive years. Field experiments were conducted, and essential oils were extracted using a Clevenger-type apparatus followed by gas chromatographic–mass spectrometric analysis for compound identification. Weather conditions during the vegetation periods were recorded, and statistical analyses, including principal component analysis (PCA) and correlation analysis, were performed to assess the volatile compound compositions. Results indicate significant correlations among various compounds within each fruit type, with distinct patterns observed across different years. PCA further elucidates the influence of both year and sampling location on the chemical profiles of essential oils. Cluster analysis reveals clustering primarily based on the year of cultivation rather than geographical location, emphasizing the dominant role of weather conditions in shaping essential oil compositions. This study highlights the intricate relationship between climate conditions and the quality of essential oils in Apiaceae fruits, providing valuable insights for optimizing cultivation practices and enhancing essential oil production. In general, climate conditions strongly influence the coriander, anise, and annual caraway cultivation, and also essential oil quality.
Reduced Root Volume at Establishment, Canopy Growth and Fruit Production in ‘Lapins’/‘Colt’ and ‘Regina’/‘Gisela 12’ Sweet Cherry Trees
Authors: Yuri José Antonio , Simeone Daniela , Fuentes Mauricio , Sepúlveda Álvaro , Palma Miguel , Moya Mariana , Sánchez-Contreras Javier
Published: June 2024

The success of establishing fruit orchards has traditionally been attributed to the vigor of the nursery plant used. This study aimed to evaluate the post-transplant survival, canopy growth and fruit productivity of two sweet cherry (Prunus avium L.) cultivars (‘Lapins’/‘Colt’ and ‘Regina’/‘Gisela 12’) with different radicular basal volumes of 100%, 50% and 25% and nursery plant types: (i) bare root (BR) or (ii) bagged (B). The initial stem diameter of the plants ranged from 12 to 19 mm, and their height ranged from 1.4 to 1.8 m. Plants grafted onto ‘Colt’ rootstock exhibited twice the initial root volume compared to those grafted onto ‘Gisela 12’. Evaluations were carried out in three commercial orchards during three seasons in the Central Valley of Chile. The results indicated that root volume and nursery type did not affect plant survival and productivity. For ‘Regina’/‘Gisela 12’, only the bag treatment resulted in less trunk cross-sectional area (TCSA) and shoot length, and for ‘Lapins’/‘Colt’, the BR25 treatment showed a lower initial TCSA than other treatments, although without a negative effect on yield. Hence, the presumption about the influence of root volume and plant type on the successful establishment of a sweet cherry on ‘Colt’ and ‘Gisela 12’ rootstock can be discarded. The survival, growth and precocity of the orchard depend more on post-planting conditions and water management than on the number or type of nursery plant roots. It is important to prioritize proper post-planting care and water management for optimal orchard health.
Pollen- and Weather-Based Machine Learning Models for Estimating Regional Olive Production
Authors: Galveias Ana , Antunes Célia , Costa Ana Rodrigues , Fraga Helder
Published: June 2024

The olive tree is one of the most common type of cultivation in the Mediterranean area, having high economic and social importance. The Alentejo region, Portugal, is an area with a high presence of olive groves, which in 2022 accounted for 201,474 hectares. The aim of this study was to assess the relationship between olive pollen, weather data, and olive tree production, between the years 2002 and 2022. Pollen data were obtained from an urban station located in Évora, in the Alentejo region, and were used to calculate several metrics, such as the Pollen Season Duration (PSD), Seasonal Pollen Index (SPIn), peak value, and weekly pollen accumulation values. Monthly minimum, maximum, and mean temperature and precipitation sums were obtained from the E-OBS observational dataset. Considering the relationship between pollen/weather and olive production, mutual information and correlation analyses were conducted. Subsequently, several machine learning algorithms were trained using pollen and weather datasets, and we obtained suitable forecast models for olive tree production after cross-validation. The results showed high variability in pollen concentrations in Évora over the years. Complex associations were found, with certain weeks of pollen accumulation showing significant mutual information with olive production, particularly during June. The analyzed linear correlation coefficients remained generally low, underscoring the challenge of predicting olive production based on linear relationships. Among the machine learning algorithms employed to predict olive production, Decision Trees, Extreme Gradient Boosting, and Gradient Boosting Regressor were the most robust performers (r2 > 0.70), while linear models displayed a subpar performance (r2 < 0.5), emphasizing the complexity of this approach. These models highlight the roles of maximum and minimum temperatures during March and May and pollen accumulation during the second half of June. The developed models may be used as decision-support tools by growers and stakeholders to further enhance the sustainability of the thriving olive sector in southern Portugal.
Comprehensive Evaluation of the ‘Shixia’ Longan Quality under Postharvest Ambient Storage: The Volatile Compounds Played a Critical Part
Authors: Li Jingyi , Luo Tao , Xu Jianhang , Zhu Difa , Han Dongmei , Wu Zhenxian
Published: June 2024

Longan fruit generally undergoes rapid quality deterioration during the postharvest stage, with the manifestation of flavor loss as well as pronounced off-odor production. Nevertheless, the unapparent aroma makes people ignore the odor change in postharvest longan. Sensory analysis serves as an indispensable method combining instrumental detection and the perceptibility of human sensation in a comprehensive evaluation of quality during production and consumption. In this study, we established the evaluating data of the appearance, flavor, taste substances, volatile profiles, and deterioration of ‘Shixia’ longan throughout room-temperature storage using instrument assessment and descriptive measurements. Our results indicated that both the appearance state and the taste condition notably engendered confusion or trouble for consumers to judge under the quality transition period. Conversely, the development of odor was highly consistent with that of quality deterioration. Some unpleasant volatile substances including alcohol (ethanol), acid (acetic acid), and esters (acetic acid methyl ester and ethyl acetate) were probably the cause of off-odor during the storage. The result of the sensory evaluation also presents a more significant relevance between the overall quality and the odor. Generally, the work paved the way to reveal the importance of odor profiles for assessing the comprehensive quality condition of postharvest room-temperature stored longan.
Evaluation of Gridded Rainfall Products in Three West African Basins
Authors: Goudiaby Omar , Bodian Ansoumana , Dezetter Alain , Diouf Ibrahima , Ogilvie Andrew
Published: June 2024

In recent years, accessing rainfall data from ground observation networks maintained by national meteorological services in West Africa has become increasingly challenging. This is primarily due to high acquisition costs and the often sparse distribution of rainfall gauges across the region, which limits their use in hydrological studies and related research. At the same time, the rising availability of precipitation products derived from satellite/earth observations, reanalysis datasets, and in situ measurements presents exciting prospects for hydrological applications. Nonetheless, these datasets constitute indirect measurements, necessitating rigorous validation against ground-based rainfall data. This study comprehensively assesses twenty-three gridded rainfall products, including sixteen from satellites, six from reanalysis data, and one from in situ measurements, across the Senegal, Gambia, and Casamance River basins. Performance evaluation is conducted across distinct climatic zones, both pre- and post-resampling against observed rainfall data gathered from forty-nine rainfall stations over a six-year period (2003–2008). Evaluation criteria include the Kling–Gupta Efficiency (KGE) and Percentage of Bias (PBIAS) metrics, assessed at daily, monthly, and seasonal time steps. The results reveal distinct performance levels among the evaluated rainfall products. RFE, ARC2, and CPC notably yield the highest KGE scores at the daily time step, while GPCP, CHIRP, CHIRPS, RFE, MSWEP, ARC2, CPC, TAMSAT, and CMORPHCRT demonstrate superior performance at the monthly time step. During the rainy season, these products generally exhibit robustness. However, rainfall estimates derived from reanalysis datasets (ERA5, EWEMBI, MERRA2, PGF, WFDEICRU, and WFDEIGPCC) perform poorly in the studied basins. Based on the PBIAS metric, most products tend to underestimate precipitation values, while only PERSIANN and PERSIANNCCS lead to significant overestimations. Spatially, optimal performance of the products is observed in the Casamance basin and the Sudanian and Sahelian climatic zones within the Gambia and Senegal basins. Conversely, in the Guinean zone of the Gambia and Senegal Rivers, the rainfall products displayed the poorest performance.
Land-Use–Land Cover Changes in the Urban River’s Buffer Zone and Variability of Discharge, Water, and Sediment Quality—A Case of Urban Catchment of the Ngerengere River in Tanzania
Authors: Mbonaga Silaji S. , Hamad Amina A. , Mkoma Stelyus L.
Published: June 2024

The physical integrity of the Ngerengere River and its three tributaries drains within Morogoro Municipality were evaluated by assessing the variations in land-use–land cover (LULC) in the river’s buffer zone, the discharge, and the contamination of river water and sediment from nutrients and heavy metals. Integrated geospatial techniques were used to classify the LULC in the river’s buffer zone. In contrast, the velocity area method and monitoring data from the Wami-Ruvu Basin were used for the discharge measurements. Furthermore, atomic absorption spectrophotometry was used during the laboratory analysis to determine the level of nutrients and heavy metals in the water and river sediment across the 13 sampling locations. The LULC assessment in the river’s buffer during the sampling year of 2023 showed that bare land and built-up areas dominate the river’s buffer, with a coverage of 28% and 38% of the area distribution. The higher discharge across the sampling stations was in the upstream reaches at 3.73 m3/s and 2.36 m3/s at the confluences. The highest concentrations of heavy metals in the water for the dry and wet seasons were 0.09 ± 0.01, 0.25 ± 0.01, 0.03 ± 0.02, 0.73 ± 0.04, 4.07 ± 0.08, and 3.07 ± 0.04 mg/L, respectively, for Pb, Cr, Cd, Cu, Zn, and Ni. The order of magnitude of the heavy metal concentration in the sediments was Zn > Ni > Cr > Cu > Cd > Pb, while the highest NO2−, NO3−, NH3, and PO43− in the water and sediment were 2.05 ± 0.01, 0.394 ± 0.527, 0.66 ± 0.05, and 0.63 ± 0.01 mg/L, and 2.64 ± 0.03, 0.63 ± 0.01, 2.36 ± 0.01, and 48.16 ± 0.01 mg/kg, respectively, across all sampling seasons. This study highlights the significant impact of urbanization on river integrity, revealing elevated levels of heavy metal contamination in both water and sediment, the variability of discharge, and alterations in the LULC in the rivers’ buffer. This study recommends the continuous monitoring of the river water quality and quantity of the urban rivers, and the overall land-use plans for conserving river ecosystems.
Specific Personal Hygiene Procedures and Practices in Food Handlers—A Cross-Sectional Study in Butcher and Fishmonger Shops in Almada
Authors: Oliveira Inês , Almeida Miguel , Gomes João J. Ferreira , Henriques Ana Rita
Published: June 2024

Good manufacturing practices play an important role in obtaining safe food and preventing foodborne diseases. To achieve this goal, food handlers must receive appropriate training to be aware of their responsibilities. In this work, compliance with specific personal hygiene requirements by food handlers was assessed in a cross-sectional study of traditional small retail establishments, namely butcher (n = 56) and fishmonger (n = 17) shops in Almada, Portugal. Food handlers (n = 140, of which 113 worked in butcher shops, and 27 worked in fishmonger shops) were interviewed for data collection, and retail establishments were audited considering specific hygiene requisites. In fishmonger shops, most food handlers are women (89%), aged 18 to 45 years (70%), with a high school degree, having worked for less than 5 years in this activity, while in butcher shops most food handlers are men (90%) over 45 years old (58%), with a basic education level, and more than 26 years of experience. Most food handlers (>95%) attended recent food safety and hygiene training courses and were able to recognize that hand sanitizers cannot replace a proper hand wash, and to identify Staphylococcus aureus transmission routes to food. However, approximately 23% of retail establishments failed to provide hot water in the handwashing basin and exhibited improper placement of handwashing instructions. Furthermore, these establishments did not implement corrective actions following non-conforming microbiological results of hand hygiene monitoring. These findings reinforce the need for consistent management commitment, and for providing food handlers with regular training, which is crucial for maintaining a strong food safety and hygiene culture in these traditional small retail establishments.
Influences on COVID-19 Vaccine Adherence among Pregnant Women: The Role of Internet Access and Pre-Vaccination Emotions
Authors: Carvalho de Sousa Rosângela , Lima da Silva Maria Juliene , Fialho do Nascimento Maria Rita , da Cruz Silveira Mayara , Fernandes Franciane de Paula , Quaresma Tatiane Costa , Aguiar da Silva Figueira Simone , Silva Ferreira Maria Goreth , Santos de Souza Adjanny Estela , Pires Moraes Waldiney , Silva de Oliveira Sheyla Mara , Valentim Livia de Aguiar
Published: June 2024

Introduction: The onset of the COVID-19 pandemic brought about global uncertainties and fears, escalating the dissemination of fake news. This study aims to analyze the impact of fake news on COVID-19 vaccine adherence among pregnant women, providing crucial insights for effective communication strategies during the pandemic. Methods: A cross-sectional, exploratory study was conducted with 113 pregnant women under care at a Women’s Health Reference Center. Data analysis included relative frequency and odds ratio to assess the relationship between sociodemographic and behavioral variables regarding vaccination. Results: In the behavioral context of vaccination, internet access shows a significant association with decision-making, influencing vaccine refusal due to online information. Nuances in the odds ratios results highlight the complexity of vaccine hesitancy, emphasizing the importance of information quality. Pre-vaccination sentiments include stress (87.61%), fear (50.44%), and anxiety (40.7%), indicating the need for sensitive communication strategies. Discussion: Results revealed that pregnant women with higher education tend to adhere more to vaccination. Exposure to news about vaccine inefficacy had a subtle association with hesitancy, while finding secure sources was negatively associated with hesitancy. The behavioral complexity in the relationship between online information access and vaccination decision underscores the need for effective communication strategies. Conclusions: In the face of this challenging scenario, proactive strategies, such as developing specific campaigns for pregnant women, are essential. These should provide clear information, debunk myths, and address doubts. A user-centered approach, understanding their needs, is crucial. Furthermore, ensuring information quality and promoting secure sources are fundamental measures to strengthen trust in vaccination and enhance long-term public health.
The Impact of Urban Allotment Gardens on Physical and Mental Health in Norway
Authors: Johnsrud Mari , Goth Ursula Småland , Skjerve Hilde
Published: June 2024

In Norway, many communities offer low-rent plots of land on which city dwellers can build summer cottages and grow crops. These allotment gardens serve as recreational escapes from urban life. However, little is known about the impact these gardens have on their members. This study attempts to shed light on today’s impact of allotment gardens in a public health setting in Norway. The study was based on 17 semi-structured interviews and 2 in-depth interviews with allotment house owners. Informants were mainly recruited by “snowball sampling”. Our data show that maintaining an allotment promotes exercise and provides a positive impact on self-perceived well-being and physical health through outdoor activities. Spending time in the garden contributes to new knowledge and experiences. Owning an allotment garden has provided new knowledge, new skills, new hobbies, and thereby an improvement in lifestyle. The allotment garden has a strong impact on perceived health, well-being, and sense of coherence (SOC) for the individuals. It promotes outdoor activities along with social interaction and can prevent feelings of loneliness and isolation.