bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Evaluation of Secreted Plant Carotenoid Cleavage Dioxygenase 1 (CCD1) Enzymes in Saccharomyces cerevisiae
Authors: Timmins John J. B. , Kroukamp Heinrich , Walker Roy S. K. , Pretorius Isak S. , Paulsen Ian T.
Published: August 2022

Enabling technologies in synthetic biology now present the opportunity to engineer wine yeast for enhanced novel aromas. In doing so, improved wine products will increase the desirability of wine for the consumer and add value to the winemaker. The action of the enzyme carotenoid cleavage dioxygenase 1 (CCD1) on β-carotene to produce β-ionone is of interest to improve the aroma and flavour of the wine. Engineering the yeast, Saccharomyces cerevisiae, to produce higher concentrations of CCD1 in grape-must presents an opportunity to increase the levels of this volatile organic compound, thus enhancing the organoleptic properties of wine. To this end, four phylogenetically diverse plant CCD1 genes were synthesised with a secretion signal peptide and transformed into S. cerevisiae. The relative ability of each enzyme secreted into the yeast supernatant to cleave the deep orange C40 β-carotene was determined by spectrophotometry; furthermore, the by-product of such cleavage, the highly aromatic C13 β-ionone, was assessed by head-space solid-phase micro-extraction, with analysis and detection by GCMS. Reduction in β-carotene levels and release of β-ionone from the supernatant were validated by LCMS detection of CCD1. These experiments demonstrated that expression in yeast of the CCD1s derived from Petunia hybrida and Vitis vinifera and their subsequent secretion into the medium provided superior efficacy in both β-carotene reduction and β-ionone liberation. We anticipate this knowledge being of benefit to future winemakers in producing a vinous product with enhanced organoleptic properties.
An Implicit Difference Scheme for the Fourth-Order Nonlinear Evolution Equation with Multi-Term Riemann–Liouvile Fractional Integral Kernels
Authors: Jiang Xiaoxuan , Yang Xuehua , Zhang Haixiang , Tian Qingqing
Published: August 2022

In this paper, an implicit difference scheme is proposed and analyzed for a class of nonlinear fourth-order equations with the multi-term Riemann–Liouvile (R–L) fractional integral kernels. For the nonlinear convection term, we handle implicitly and attain a system of nonlinear algebraic equations by using the Galerkin method based on piecewise linear test functions. The Riemann–Liouvile fractional integral terms are treated by convolution quadrature. In order to obtain a fully discrete method, the standard central difference approximation is used to discretize the spatial derivative. The stability and convergence are rigorously proved by the discrete energy method. In addition, the existence and uniqueness of numerical solutions for nonlinear systems are proved strictly. Additionally, we introduce and compare the Besse relaxation algorithm, the Newton iterative method, and the linearized iterative algorithm for solving the nonlinear systems. Numerical results confirm the theoretical analysis and show the effectiveness of the method.
Temperature-Ion-pH Triple Responsive Gellan Gum as In Situ Hydrogel for Long-Acting Cancer Treatment
Authors: Zhou Shuwen , Zheng Xinmeng , Yi Ke , Du Xuancheng , Wang Cheng , Cui Pengfei , Jiang Pengju , Ni Xinye , Qiu Lin , Wang Jianhao
Published: August 2022

Background: Promising cancer chemotherapy requires the development of suitable drug delivery systems (DDSs). Previous research has indicated that a hydrogel is a powerful DDS for tumor therapy and holds great potential to offer a feasible method for cancer management. Methods: In this study, glutathione-gellan gum conjugate (GSH-GG) was synthesized through chemical reaction. Doxorubicin hydrochloride (DOX) was loaded into GSH-GG to accomplish DOX-loaded GSH-GG. The properties, injectability, drug release, and in vitro and in vivo anticancer effects of DOX-loaded GSH-GG were tested. Results: DOX-loaded GSH-GG showed a temperature-ion dual responsive gelling property with good viscosity, strength, and injectability at an optimized gel concentration of 1.5%. In addition, lower drug release was found under acidic conditions, offering beneficial long-acting drug release in the tumor microenvironment. DOX-loaded GSH-GG presented selective action by exerting substantially higher cytotoxicity on cancer cells (4T1) than on normal epithelial cells (L929), signifying the potential of complete inhibition of tumor progression, without affecting the health quality of the subjects. Conclusions: GSH-GG can be applied as a responsive gelling material for delivering DOX for promising cancer therapy.
Exploring Readiness towards Effective Implementation of Safety and Health Measures for COVID-19 Prevention in Nakhon-Si-Thammarat Community-Based Tourism of Southern Thailand
Authors: Bumyut Apirak , Thanapop Sasithorn , Suwankhong Dusanee
Published: August 2022

Thailand’s community-based tourism (CBT) faces a challenging adaptation in response to COVID-19 prevention. This study aimed to assess the readiness for effective implementation of the Safety and Health Administration (SHA) for COVID-19 prevention in the tourism community. A qualitative approach was adopted for this study. Three communities covering all types of CBT in Nakhon-Si-Thammarat province, southern Thailand were purposively chosen. Fifteen key informants were invited to participate in the study. Semi-structured in-depth interviews were conducted, and the data were analysed using the thematic analysis method. The readiness stage was assigned by consensual comprehensive scores. The overall readiness of CBT is pre-planning stage, a clear recognition of the SHA benefit, and there are sufficient resources for implementation. At this stage, there is no planning because the business owners feel that they have inadequate knowledge about the SHA protocol. Another main barrier is having limited accessibility for SHA information which mainly provides through with technology platform. The CBT owner needs to improve public health-based knowledge, technology and cooperation skills to operate SHA efficiently. However, in order to embed SHA to the CBT, tourism and public health organisation should provide suitable methods at the initiation stage by considering the community readiness and need.
Association between Workers’ Anxiety over Technological Automation and Sleep Disturbance: Results from a Nationally Representative Survey
Authors: Baek Seong-Uk , Yoon Jin-Ha , Won Jong-Uk
Published: August 2022

Despite the positive aspects of recent technological innovations, fears are mounting among workers that machines will inevitably replace most human jobs in the future. This study is the first to explore the association between individual-level automation anxiety and insomnia among workers. We scored the worker’s anxiety over technological automation with five questions. The total sum of scores for participants was categorized in quartiles (Q1–Q4). Logistic regression was employed to estimate odds ratios (ORs) and confidence intervals (CIs). The highest scoring group (Q4) had the highest OR for sleep disturbance (OR [95% CI]:1.40 [1.27–1.55]) compared to the lowest scoring group (Q1). ORs of the highest scoring group (Q4) were strongest for the young (OR [95% CI]:1.96 [1.52–2.53]), followed by the middle-aged (OR [95% CI]:1.40 [1.20–1.64]), and old age groups (OR [95% CI]:1.29 [1.10–1.51]). In addition, a 1-point increase in the automation anxiety score had the strongest association with sleep disturbance in the young (OR [95% CI]:1.07 [1.05–1.10]), followed by the middle-aged (OR [95% CI]:1.03 [1.02–1.04]), and old age groups (OR [95% CI]:1.02 [1.01–1.04]). Our study suggests that policies such as worker retraining are needed to alleviate workers’ undue anxiety.
Risk of Peripheral Arterial Occlusive Disease with Periodontitis and Dental Scaling: A Nationwide Population-Based Cohort Study
Authors: Yeh Ying-Ting , Tseng Yen-Shuo , Wu Yi-Liang , Yang Shun-Fa , Wang Bo-Yuan , Wang Yu-Hsun , Yeh Liang-Tsai , Yeh Ying-Tung , Chan Chi-Ho
Published: August 2022

Periodontitis (PD) is a common oral disease associated with various other diseases, particularly those affecting the cardiovascular system. This study explored whether peripheral artery occlusive disease (PAOD) is associated with PD and dental scaling. This study was a retrospective cohort study design from 2000 to 2018. The study population was newly diagnosed with periodontitis. The comparison group was defined as never diagnosed with periodontitis. The outcome variable was defined with the diagnosis of peripheral arterial occlusive disease (PAOD). The propensity score matching was performed by age, sex, comorbidities, and dental scaling between the two groups. Kaplan–Meier analysis was used to calculate the cumulative incidence of PAOD among the two groups. To perform the independent risk of the PAOD group, the multivariate Cox proportional hazard model was used to estimate the hazard ratios. First, 792,681 patients with PD and 458,521 patients with no history of PD were selected from Taiwan’s Longitudinal Health Insurance Database, which comprises the data of two million beneficiaries. After propensity score matching between the PD and non-PD groups for age, sex, comorbidities, and dental scaling, 357,106 patients in each group were analyzed for PAOD risk. The incidence density, relative risk, and cumulative incidence of PAOD were higher in the PD group than in the non-PD group. After adjusting for all variables, the risk of PAOD for the PD group was greater than for the non-PD group (adjusted hazard ratio = 1.03; 95% CI, 1.01–1.06). Undergoing at least one dental scaling procedure reduced the risk of PAOD. Age over 65 years was also a risk factor. In conclusion, patients with PD have an increased risk of PAOD. In addition, our results can lead to increased attention to oral hygiene, as dental scaling has a trend towards a lower risk of PAOD.
Travel Behaviour of Vulnerable Social Groups: Pre, during, and Post COVID-19 Pandemic
Authors: Dadashzadeh Nima , Larimian Taimaz , Levifve Ulysse , Marsetič Rok
Published: August 2022

Since the emergence of COVID-19, travel restrictions due to the pandemic have influenced several activities, in particular the mobility patterns of individuals. Our main goal is to draw the attention of scholars and policy makers to a specific segment of the population, namely (1) older people, (2) persons with disabilities (PwDs), (3) females, and (4) low-income population that are more vulnerable for travel behaviour change due to crisis such as the COVID-19 pandemic. This article systematically reviews the studies that have explored the implications of COVID-19 for the mobility and activities of individuals pre-, during, and post-pandemic using the PRISMA method. It is found that there are a few studies regarding the travel and mobility needs and challenges of older people and PwDs, and there is no direct study concerning female and low-income individuals while such crisis exist. Questions such as “What are the adverse impacts of restrictions on their travel behaviour?”, “How can they travel safely to work, shopping, and medical centres?”, “Which transportation modes can be more effective for them?”, and “What are the government and policy makers’ role in providing accessible and affordable mobility services in the presence of such crisis?” are without relevant answers in the literature.
Does the Association of Sedentary Time or Fruit/Vegetable Intake with Central Obesity Depend on Menopausal Status among Women?
Authors: Su Jing , Li Qingting , Mao Ping , Peng Hua , Han Huiwu , Wiley James , Guo Jia , Chen Jyu-Lin
Published: August 2022

(1) Background: The prevalence of central obesity is growing rapidly among women, and the prevalence differs by menopausal status. Longer sedentary time and less fruit/vegetable (F/V) intake increased the risk of central obesity. Among women of different menopausal statuses, controversy surrounds the association between sedentary time or F/V intake and central obesity. This study aimed to explore whether the independent and joint associations between sedentary time or F/V intake and menopausal status are correlated with central obesity, respectively. (2) Methods: This cross-sectional study was conducted in Hunan, China. Self-reported questionnaires were used to gather information on demographic characteristics, menopausal status, sedentary time, and F/V intake. Waist circumference was measured at the study site. Binary logistic regression and multiple interaction models were used to explore the independent and joint associations of menopausal status and the above two lifestyle variables with central obesity. (3) Results: A total of 387 women with a mean age of 47.7 ± 6.6 years old participated in the study. The prevalence of central obesity was 52.8%. Peri- and post-menopause statuses and not taking five servings of F/V per day were risk factors of central obesity (p < 0.05), whereas no significant association was found between sedentary time and central obesity (p > 0.05). Among peri-menopausal (mutual odds ratio (OR): 2.466, 95% confidence interval [CI]: 0.984–6.182; p < 0.05) and post-menopausal women (mutual OR: 2.274, 95% CI: 1.046–4.943; p < 0.05), more than 4 h of sedentary time per day was associated with a high risk of central obesity. Among pre-menopausal women, the consumption of five servings of F/V per day was associated with a low risk of central obesity (mutual OR: 0.444, 95%CI: 0.236–0.837, p < 0.05). (4) Conclusions: More than half of women in the central south of China presented with central obesity, spent >4 h of sedentary time per day, or did not engage in recommended F/V intake. Healthier lifestyle intervention is warranted to prevent central obesity development, including reducing the sedentary time to <4 h per day for peri- and post-menopausal women, while increasing taking five servings of F/V per day for pre-menopausal women.
Gender-Neutral Toilets: A Qualitative Exploration of Inclusive School Environments for Sexuality and Gender Diverse Youth in Western Australia
Authors: Francis Jacinta , Sachan Pratishtha , Waters Zoe , Trapp Gina , Pearce Natasha , Burns Sharyn , Lin Ashleigh , Cross Donna
Published: August 2022

School toilets have been identified by sexuality and gender diverse (SGD) students as the least safe spaces in educational institutions. They are sites of verbal, physical and sexual victimisation. Providing gender-neutral toilets in primary and secondary schools may reduce the bullying and victimisation of SGD students, particularly those who are transgender or gender-diverse. This study explored factors influencing the inclusion of gender-neutral toilets in primary and secondary schools in Western Australia. Thirty-four interviews were conducted from May to December 2020 with policy makers or practitioners (n = 22) and school staff (n = 12) in Perth, Western Australia. Interviews were conducted online and face-to-face using semi-structured interview guides. A thematic analysis of the cross-sectional qualitative data was undertaken. School staff, policy makers, and practitioners identified school toilets as sites of bullying and victimisation of SGD youth and expressed support for gender-neutral toilets as an anti-bullying strategy. Perceived barriers to introducing gender-neutral toilets in schools included financial and spatial costs, building code compliance constraints, resistance from parents and students, privacy and confidentiality concerns, and cultural appropriateness. Including gender-neutral toilets in schools may reduce school-based bullying and victimisation, and improve the mental and physical health of SGD youth.
Obstetric Complications in Women from Sub-Saharan Africa—A Cross-Sectional Study
Authors: Gombau-Giménez Laura , Almansa-Martínez Pilar , Suarez-Cortés María , Molina-Rodríguez Alonso , Leal-Costa César , Jiménez-Ruiz Ismael
Published: August 2022

Aim: The aim of this study was to identify and compare birthing complications in women originating from countries where they are at risk (may become victims) of FGM with those of Spanish women, all having given birth from 2012 to 2015 at the “Virgen de la Arrixaca” University Clinical Hospital in Murcia, Spain. Methods: A transversal, observational, quantitative study was carried out, retrospectively, comparing 245 sub-Saharan women originating from countries where FGM is practiced with 490 Spanish women, in terms of obstetric complications. Data collection was performed via electronic clinical records. Results: The sub-Saharan women presented higher rates of intrapartum and emergency caesareans, intense postpartum haemorrhages, concurrent episiotomies and tears (2nd and 3rd degree), failed inductions, and non-progressive labours, and a more severe risk of foetal distress when compared with Spanish women. Conclusions: The fact that the sub-Saharan women originating from countries where FGM is practiced presented a greater number of birthing complications than the Spanish women proves the need for Spanish healthcare professionals to receive training towards cultural competency acquisition, in order to provide a multidisciplinary approach, with standardized action protocols focused fundamentally on prevention.
Vitamin D and the Kidney: Two Players, One Console
Authors: Zappulo Fulvia , Cappuccilli Maria , Cingolani Alessandra , Scrivo Anna , Chiocchini Anna Laura Croci , Nunzio Miriam Di , Donadei Chiara , Napoli Marianna , Tondolo Francesco , Cianciolo Giuseppe , La Manna Gaetano
Published: August 2022

Vitamin D belongs to the group of liposoluble steroids mainly involved in bone metabolism by modulating calcium and phosphorus absorption or reabsorption at various levels, as well as parathyroid hormone production. Recent evidence has shown the extra-bone effects of vitamin D, including glucose homeostasis, cardiovascular protection, and anti-inflammatory and antiproliferative effects. This narrative review provides an overall view of vitamin D’s role in different settings, with a special focus on chronic kidney disease and kidney transplant.
Prokaryotic Na+/H+ Exchangers—Transport Mechanism and Essential Residues
Authors: Patiño-Ruiz Miyer , Ganea Constanța , Călinescu Octavian
Published: August 2022

Na+/H+ exchangers are essential for Na+ and pH homeostasis in all organisms. Human Na+/H+ exchangers are of high medical interest, and insights into their structure and function are aided by the investigation of prokaryotic homologues. Most prokaryotic Na+/H+ exchangers belong to either the Cation/Proton Antiporter (CPA) superfamily, the Ion Transport (IT) superfamily, or the Na+-translocating Mrp transporter superfamily. Several structures have been solved so far for CPA and Mrp members, but none for the IT members. NhaA from E. coli has served as the prototype of Na+/H+ exchangers due to the high amount of structural and functional data available. Recent structures from other CPA exchangers, together with diverse functional information, have allowed elucidation of some common working principles shared by Na+/H+ exchangers from different families, such as the type of residues involved in the substrate binding and even a simple mechanism sufficient to explain the pH regulation in the CPA and IT superfamilies. Here, we review several aspects of prokaryotic Na+/H+ exchanger structure and function, discussing the similarities and differences between different transporters, with a focus on the CPA and IT exchangers. We also discuss the proposed transport mechanisms for Na+/H+ exchangers that explain their highly pH-regulated activity profile.
Modulation of KV4.3-KChIP2 Channels by IQM-266: Role of DPP6 and KCNE2
Authors: de Benito-Bueno Angela , Socuellamos Paula G. , Merinero Yaiza G. , Cercos Pilar , Izquierdo Carolina , Daniel-Mozo Miguel , Marín-Olivero Irene , Perez-Lara Angel , Gonzalez-Vera Juan A. , Orte Angel , Albert Armando , Martin-Martinez Mercedes , Gutierrez-Rodriguez Marta , Valenzuela Carmen
Published: August 2022

The transient outward potassium current (Itof) is generated by the activation of KV4 channels assembled with KChIP2 and other accessory subunits (DPP6 and KCNE2). To test the hypothesis that these subunits modify the channel pharmacology, we analyzed the electrophysiological effects of (3-(2-(3-phenoxyphenyl)acetamido)-2-naphthoic acid) (IQM-266), a new KChIP2 ligand, on the currents generated by KV4.3/KChIP2, KV4.3/KChIP2/DPP6 and KV4.3/KChIP2/KCNE2 channels. CHO cells were transiently transfected with cDNAs codifying for different proteins (KV4.3/KChIP2, KV4.3/KChIP2/DPP6 or KV4.3/KChIP2/KCNE2), and the potassium currents were recorded using the whole-cell patch-clamp technique. IQM-266 decreased the maximum peak of KV4.3/KChIP2, KV4.3/KChIP2/DPP6 and KV4.3/KChIP2/KCNE2 currents, slowing their time course of inactivation in a concentration-, voltage-, time- and use-dependent manner. IQM-266 produced an increase in the charge in KV4.3/KChIP2 channels that was intensified when DPP6 was present and abolished in the presence of KCNE2. IQM-266 induced an activation unblocking effect during the application of trains of pulses to cells expressing KV4.3/KChIP2 and KV4.3/KChIP2/KCNE2, but not in KV4.3/KChIP2/DPP6 channels. Overall, all these results are consistent with a preferential IQM-266 binding to an active closed state of Kv4.3/KChIP2 and Kv4.3/KChIP2/KCNE2 channels, whereas in the presence of DPP6, IQM-266 binds preferentially to an inactivated state. In conclusion, DPP6 and KCNE2 modify the pharmacological response of KV4.3/KChIP2 channels to IQM-266.
Genotypes and Variants of BKPyV in Organ Donors after Brain Death
Authors: Furmaga Jacek , Kowalczyk Marek , Furmaga-Rokou Olga , Rokos Christos A. , Zapolski Tomasz , Krakowski Leszek , Jakubczak Andrzej , Rudzki Sławomir
Published: August 2022

Kidney transplantation from a donor with latent BKPyV might be the cause of serious complications, such as BK virus-associated nephropathy. The aim of the study was to determine the prevalence of BKPyV infection in donors after brain death (DBDs), to analyse the molecular variation of BKPyV and to compare clinical and inflammation parameters of DBDs infected with various genotypes of BKPyV. BKPyV was investigated in blood and urine samples of 103 DBDs using PCR followed by sequencing and bioinformatic analysis, and the viral load was assessed by qPCR. Clinical parameters, including cellular markers of inflammation were assessed. The results confirm high prevalence of BKPyV (48%),and genotype IV (49%) over genotype I (43%) and the co-infection with genotypes I and IV in 8.2%. Viral load ranged from 102 to 107 copies/mL, with an average of 1.92 × 106 copies/mL. No specific markers for BKPyV infection were detected among the parameters tested. Infection with genotype I may be associated with the adverse impact on thekidney function, while infection with genotype IV was associated with the anemia Not only the viral load but also the genotype of BKPyV may have an impact on the course of infection.
Acetylated Tau Protein: A New Piece in the Puzzle between Brain Ischemia and Alzheimer’s Disease
Authors: Pluta Ryszard , Januszewski Sławomir , Jabłoński Mirosław
Published: August 2022

Cerebral ischemia in humans and animals is a life-threatening neuropathological event and leads to the development of dementia with the Alzheimer’s disease phenotype [...]
Identification of Gene Modules and Hub Genes Associated with Sporisorium scitamineum Infection Using Weighted Gene Co-Expression Network Analysis
Authors: Liu Zongling , Li Xiufang , Li Jie , Zhao Haiyun , Deng Xingli , Su Yizu , Li Ru , Chen Baoshan
Published: August 2022

Sporisorium scitamineum is a biotrophic fungus responsible for sugarcane smut disease. To investigate the key genes involved in S. scitamineum infection, we conducted RNA sequencing of sugarcane sprouts inoculated with S. scitamineum teliospores. A weighted gene co-expression network analysis (WGCNA) showed that two co-expressed gene modules, MEdarkturquoise and MEpurple—containing 66 and 208 genes, respectively—were associated with S. scitamineum infection. The genes in these two modules were further studied using Gene Ontology (GO) enrichment analysis, pathogen-host interaction (PHI) database BLASTp, and small secreted cysteine-rich proteins (SCRPs) prediction. The top ten hub genes in each module were identified using the Cytohubba plugin. The GO enrichment analysis found that endoplasmic reticulum-related and catabolism-related genes were expressed during S. scitamineum infection. A total of 83 genes had homologs in the PHI database, 62 of which correlated with pathogen virulence. A total of 21 proteins had the characteristics of small secreted cysteine-rich proteins (SCRPs), a common source of fungal effectors. The top ten hub genes in each module were identified, and seven were annotated as Mig1-Mig1 protein, glycosyl hydrolase, beta-N-acetylglucosaminidase, secreted chorismate mutase, collagen, mRNA export factor, and pleckstrin homology domain protein, while the remaining three were unknown. Two SCRPs—SPSC_06609 and SPSC_04676—and three proteins—SPSC_01958, SPSC_02155, and SPSC_00940—identified in the PHI database were also among the top ten hub genes in the MEdarkturquoise and MEpurple modules, suggesting that they may play important roles in S. scitamineum infection. A S. scitamineum infection model was postulated based on current findings. These findings help to deepen the current understanding of early events in S. scitamineum infection.
Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity
Authors: Šurín Hudáková Natália , Kačírová Jana , Sondorová Miriam , Šelianová Svetlana , Mucha Rastislav , Maďar Marián
Published: August 2022

Bacillus licheniformis is used in a broad spectrum of areas, including some probiotic preparations for human and veterinary health. Moreover, B. licheniformis strains are known producers of various bioactive substances with antimicrobial and antibiofilm effects. In searching for new potentially beneficial bacteria for oral health, the inhibitory effect of B. licheniformis strains isolated from canine dental biofilm against pathogenic oral bacteria was evaluated. The antimicrobial effect of neutralized cell-free supernatants (nCFS) was assessed in vitro on polystyrene microtiter plates. Furthermore, molecular and morphological analyses were executed to evaluate the production of bioactive substances. To determine the nature of antimicrobial substance present in nCFS of B. licheniformis A-1-5B-AP, nCFS was exposed to the activity of various enzymes. The nCFS of B. licheniformis A-1-5B-AP significantly (p < 0.0001) reduced the growth of Porphyromonas gulae 3/H, Prevotella intermedia 1/P and Streptococcus mutans ATCC 35668. On the other hand, B. licheniformis A-2-11B-AP only significantly (p < 0.0001) inhibited the growth of P. intermedia 1/P and S. mutans ATCC 35668. However, enzyme-treated nCFS of B. licheniformis A-1-5B-AP did not lose its antimicrobial effect and significantly (p < 0.0001) inhibited the growth of Micrococcus luteus DSM 1790. Further studies are needed for the identification of antimicrobial substances.
Association of NT-proBNP and hs-cTnT with Imaging Markers of Diastolic Dysfunction and Focal Myocardial Fibrosis in Hypertrophic Cardiomyopathy
Authors: Chevalier Céleste , Wendner Miriam , Suling Anna , Cavus Ersin , Muellerleile Kai , Lund Gunnar , Kirchhof Paulus , Patten Monica
Published: August 2022

Serum biomarkers such as N-terminal prohormone of the brain natriuretic peptide (NT-proBNP) and cardiac troponins are elevated in patients with hypertrophic cardiomyopathy (HCM). At present, it is not clear if these markers are associated with distinct clinical alterations in HCM, such as left ventricular hypertrophy, outflow tract obstruction, myocardial fibrosis and/or diastolic dysfunction (DD), which are associated with adverse cardiovascular outcome. Here we evaluate the association of NT-proBNP and high sensitivity cardiac troponin T (hs-cTnT) to a variety of cardiac imaging parameters in HCM patients in a multivariable regression analysis. This retrospective cross-sectional study included 366 HCM patients who underwent transthoracic echocardiography (TTE), 218 of whom also obtained cardiovascular magnetic resonance (CMR) to assess focal myocardial fibrosis by LGE. Multivariable regression analyses revealed the strongest association of the DD parameters E/E′ mean and E/E′ septal with NT-proBNP (b = 0.06, 95%-CI [0.05–0.07], p < 0.001, R2 = 0.28; b = 0.08, 95%-CI [0.06–0.1], p < 0.001, R2 = 0.25) and LGE size showed the strongest association with hs-cTnT (b = 0.20, 95%-CI [0.15–0.24], p < 0.001, R2 = 0.28). This study indicates that NT-proBNP and hs-cTnT are associated with structural and functional alterations in HCM. NT-proBNP is a stronger predictor for DD, while hs-cTnT is associated with the extent of focal myocardial fibrosis. Both biomarkers might be useful in the diagnostic procedure in addition to imaging parameters.
Preparation and Properties of Ce0.8Sm0.16Y0.03Gd0.01O1.9-BaIn0.3Ti0.7O2.85 Composite Electrolyte
Authors: Wang Yajun , Tian Changan , Zhu Minzheng , Yang Jie , Qu Xiaoling , Chen Cao , Wang Cao , Liu Yang
Published: August 2022

Samarium, gadolinium, and yttrium co-doped ceria (Ce0.8Sm0.16Y0.03Gd0.01O1.9, CSYG) and BaIn0.3Ti0.7O2.85 (BIT07) powders were prepared by sol-gel and solid-state reaction methods, respectively. CSYG-BIT07 composite materials were obtained by mechanically mixing the two powders in different ratios and calcining at 1300 °C for 5 h. Samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), as well as electrical properties and thermal expansion coefficient (TEC) measurements. A series of CSYG-BIT07 composite materials with relative densities higher than 95% were fabricated by sintering at 1300 °C for 5 h. The performance of the CSYG-BIT07 composite electrolyte was found to be related to the content of BIT07. The CSYG-15% BIT07 composite exhibited high oxide ion conductivity (σ800°C = 0.0126 S·cm−1 at 800 °C), moderate thermal expansion (TEC = 9.13 × 10−6/K between room temperature and 800 °C), and low electrical activation energy (Ea = 0.89 eV). These preliminary results indicate that the CSYG-BIT07 material is a promising electrolyte for intermediate-temperature solid oxide fuel cells (IT-SOFCs).
Dynamic Response of UHMW-PE Composite Armors under Ballistic Impact of Blunt Projectiles
Authors: Ding Li , Gu Xiaohui , Shen Peihui , Kong Xiangsheng , Zhou Yi
Published: August 2022

To study the dynamic response of UHMW-PE composite armor under ballistic impact, two kinds of UHMW-PE composite armors are designed. Both of them are composed of UHMW-PE laminates and steel face sheets of Q235. The blunt projectile is made of 35CrMnSiA, with a cylinder shape. By numerical simulation, the dynamic response and deformation of composite armors are obtained under the penetration of the projectile. With the increase of impact velocity, the penetration depth increases nearly linearly, with a more severe tendency of swaging in the projectile. Then, experiments are carried out to validate the numerical simulation results. Based on a ballistic gun with a caliber of 14.5 mm, the projectiles are fired with a velocity from 680 m/s to 1300 m/s. The penetration into the composite armor can be divided into an initial shear plugging stage and the following bulging and delamination stage. Based on the theoretical analysis, the shear strength in the shear plugging stage can be estimated. Associated with typical experimental results, numerical simulation is suitable to predict the bulging characteristics of the composite armor. The failure mode of the composite armors under the impact of blunt projectiles is determined, and the failure mechanism is analyzed. The penetration results in the experiment agree well with the numerical simulation results, which validate the correctness of the numerical simulation models. The research results can be significant in the design of composite armor with UHMW-PE laminates.
Study on the Aging Behavior of an Ultra-High Molecular Weight Polyethylene Fiber Barrier Net in a Marine Environment
Authors: Zhang Wangxuan , Jing Xiaofei , Bai Yanqiang , Shan Xiaoming , Qi Xiaoyu , Yan Maoxin , Cui Zhongyu
Published: August 2022

In the present work, the performance of ultra-high molecular weight polyethylene (UHMWPE) barrier nets in marine environments is investigated by Fourier transform infrared spectroscopy, thermogravimetry, scanning electron microscopy, and tensile experiments. The chemical, morphological, thermal stability, and strength changes after aging in salt spray, hygrothermal, and ultraviolet (UV) environments are characterized. An environmental spectrum is designed to simulate a real service environment and predict the service life of UHMWPE. The results show that UV energy can activate UHMWPE molecules and lead to chain breaking, which lowers the breaking strength more efficiently than salt spray. In a hygrothermal environment, the UHMPE fibers bond into clumps, which causes a slight increase in breaking strength after the initial rapid decrease with aging time. The acceleration ratio of the environmental spectrum increases with increasing aging time, which may be caused by the cross-linking and degradation of macromolecular chains in the material. The environmental spectrum given by this work can be used to evaluate performance and predict the service life of UHMWPE barrier nets in marine environments.
Additively Manufactured Hierarchical Auxetic Mechanical Metamaterials
Authors: Mazur Ekaterina , Shishkovsky Igor
Published: August 2022

Due to the ability to create structures with complex geometry at micro- and nanoscales, modern additive technologies make it possible to produce artificial materials (metamaterials) with properties different from those of conventional materials found in nature. One of the classes with special properties is auxetic materials—materials with a negative Poisson’s ratio. In the review, we collect research results on the properties of auxetics, based on analytical, experimental and numerical methods. Special attention of this review is paid to the consideration of the results obtained in studies of hierarchical auxetic materials. The wide interest in the hierarchical subclass of auxetics is explained by the additional advantages of structures, such as more flexible adjustment of the desired mechanical characteristics (the porosity, stiffness, specific energy absorption, degree of material release, etc.). Possibilities of biomedical applications of hierarchical auxetic materials, such as coronary stents, filtration and drug delivery systems, implants and many others, where the ability for high-precision tuning is required, are underlined.
Tunable Compact Metamaterial-Based Double-Negative/Near-Zero Index Resonator for 6G Terahertz Wireless Applications
Authors: Musaed Alya Ali , Al-Bawri Samir Salem , Islam Mohammad Tariqul , Al-Gburi Ahmed Jamal Abdullah , Singh Mandeep Jit
Published: August 2022

This paper introduces the tunability performance, concept, and analysis of a unique and miniaturized metamaterial (MTM) unit cell covering the upcoming 6G applications. The proposed metamaterial consists of two metallic star-shaped split-ring resonators (SRR). It has a line segment placed in the middle of the structure, which can feature tunable characteristics. The proposed design provides dual resonances of transmission coefficient S21 at 0.248 and 0.383 THz with a significant operating frequency span of 0.207–0.277 and 0.382–0.390 THz, respectively. Moreover, wide-range achievement, negative permittivity, double-negative (DNG) refractive index, and near-zero permeability characteristics have been exhibited in two (z and y) principal wave propagation axes. The resonance frequencies are selective and modified by adjusting the central slotted-strip line length. Furthermore, the metamaterial is constituted on a polyimide substrate while the overall dimensions are 160 × 160 μm2. A numerical simulation of the proposed design is executed in CST microwave studio and has been compared with advanced design software (ADS) to generate the proposed MTM’s equivalent circuit, which exhibits a similar transmission coefficient (S21).
The Discovery, Enzymatic Characterization and Functional Analysis of a Newly Isolated Chitinase from Marine-Derived Fungus Aspergillus fumigatus df347
Authors: Wu Ya-Li , Wang Sheng , Yang Deng-Feng , Yang Li-Yan , Wang Qing-Yan , Yu Jun , Li Nan , Pan Li-Xia
Published: August 2022

In order to discover a broad-specificity and high stability chitinase, a marine fungus, Aspergillus fumigatus df347, was identified in the sediments of mangrove wetlands in Qinzhou Bay, China. The chitinase gene (AfChi28) from A. fumigatus df347 was cloned and heterologously expressed in Escherichia coli, and the recombinant enzyme AfChi28 was purified and characterized. AfChi28 is an acido-halotolerant- and temperature-resistant bifunctional enzyme with both endo- and exo-cleavage functions. Its enzymatic products are mainly GlcNAc, (GlcNAc)2, (GlcNAc)3 and (GlcNAc)4. Na+, Mg2+, K+, Ca2+ and Tris at a concentration of 50 mM had a strong stimulatory effect on AfChi28. The crude enzyme and pure enzyme exhibited the highest specific activity of 0.737 mU/mg and 52.414 mU/mg towards colloidal chitin. The DxDxE motif at the end of strand β5 and with Glu154 as the catalytic residue was verified by the AlphaFold2 prediction and sequence alignment of homologous proteins. Moreover, the results of molecular docking showed that molecular modeling of chitohexaose was shown to bind to AfChi28 in subsites −4 to +2 in the deep groove substrate-binding pocket. This study demonstrates that AfChi28 is a promising chitinase for the preparation of desirable chitin oligosaccharides, and provides a foundation for elucidating the catalytic mechanism of chitinases from marine fungi.
Antioxidant Potential of Sea Cucumbers and Their Beneficial Effects on Human Health
Authors: Hossain Abul , Dave Deepika , Shahidi Fereidoon
Published: August 2022

Sea cucumbers are considered a luxury food item and used locally in traditional medication due to their impressive nutritional profile and curative effects. Sea cucumbers contain a wide range of bioactive compounds, namely phenolics, polysaccharides, proteins (collagen and peptides), carotenoids, and saponins, demonstrating strong antioxidant and other activities. In particular, phenolic compounds, mainly phenolic acids and flavonoids, are abundant in this marine invertebrate and exhibit antioxidant activity. Protein hydrolysates and peptides obtained from sea cucumbers exhibit antioxidant potential, mainly dependent on the amino acid compositions and sequences as well as molecular weight, displayed for those of ≤20 kDa. Moreover, the antioxidant activity of sea cucumber polysaccharides, including fucosylated chondroitin sulfate and fucan, is a combination of numerous factors and is mostly associated with molecular weight, degree of sulfation, and type of major sugars. However, the activity of these bioactive compounds typically depends on the sea cucumber species, harvesting location, food habit, body part, and processing methods employed. This review summarizes the antioxidant activity of bioactive compounds obtained from sea cucumbers and their by-products for the first time. The mechanism of actions, chemical structures, and factors affecting the antioxidant activity are also discussed, along with the associated health benefits.
Oral Papillomatosis: Its Relation with Human Papilloma Virus Infection and Local Immunity—An Update
Authors: Andrei Elena Cristina , Baniță Ileana Monica , Munteanu Maria Cristina , Busuioc Cristina Jana , Mateescu Garofița Olivia , Mălin Ramona Denise , Pisoschi Cătălina Gabriela
Published: August 2022

Oral papilloma lesions may appear as a result of HPV infection, or not, and only special molecular methods could differentiate them. Low-risk and high-risk HPV types could induce oral HPV papillomatosis with different natural evolution, clearance and persistence mechanisms. The pathogenic mechanisms are based on the crosstalk between the oral epithelial and immune cells and this very efficient virus. HPV acts as a direct inducer in the process of transforming a benign lesion into a malignant one, the cancerization process being also debated in this paper. According to the degree of malignity, three types of papillomatous lesions can be described in the oral cavity: benign lesions, potential malign disorders and malignant lesions. The precise molecular diagnostic is important to identify the presence of various virus types and also the virus products responsible for its oncogenicity. An accurate diagnostic of oral papilloma can be established through a good knowledge of etiological and epidemiological factors, clinical examination and laboratory tests. This review intends to update the pathogenic mechanisms driving the macroscopic and histological features of oral papillomatosis having HPV infection as the main etiological factor, focusing on its interreference in the local immunity. In the absence of an accurate molecular diagnostic and knowledge of local immunological conditions, the therapeutic strategy could be difficult to decide.
Oxidative-Stress Related Gene Polymorphism in Endometriosis-Associated Infertility
Authors: Irimia Traian , Pușcașiu Lucian , Mitranovici Melinda-Ildiko , Crișan Andrada , Budianu Mihaela Alexandra , Bănescu Claudia , Chiorean Diana Maria , Niculescu Raluca , Sabău Adrian-Horațiu , Cocuz Iuliu-Gabriel , Cotoi Ovidiu Simion
Published: August 2022

Background and Objectives: Endometriosis is a benign inflammatory disease associated with infertility and chronic pelvic pain, estimated to affect 7–10% of reproductive-age women, with the possibility of malignant transformation. Recent studies focus on oxidative stress and genetic mutations as risk factors in the pathophysiology of endometriosis-associated infertility. Materials and Methods: This case-control study is the first in Eastern European women that aimed to investigate four genes’ genetic polymorphisms that encode antioxidant enzymes involved in oxidative stress (glutathione peroxidase 1, GPX1 198Pro > Leu, catalase CAT-262C > T, glutathione S-transferase M1, and T1 null genotype) and their association with endometriosis-related infertility. We compared 103 patients with endometriosis-associated infertility with 102 post-partum women as the control group. Results: The endometriosis group had a mean age of 34.5 +/− 6.12 years, while the control group’s mean age was 35.03 +/− 5.95 years. For CAT-262C > T polymorphism, the variant genotypes were significantly more frequent in the endometriosis group. Moreover, for the GPX1 198Pro > Leu, the endometriosis group had significantly more frequent CT and TT genotypes. The null genotype of GSTM1 was detected significantly higher in the endometriosis group. No significant differences were found in the frequency of GSTT1 between the two groups. This study suggests that GPX1 198Pro > Leu, CAT-262C > T, and GSTM1 polymorphisms may be risk factors and that the association between the GSTM1-GSTT1 null genotype may play a significant role in endometriosis-associated infertility. Moreover, this study suggests that the GSTT1 null genotype does not influence the disease. Visual identification of endometriotic lesions with microscopic confirmation is the accepted gold standard for diagnosing endometriosis, but general anesthesia and laparoscopy are required. Conclusions: In this regard, a panel of genetic or laboratory markers is needed for the early diagnostics of this prevalent disease, especially in the case of young patients with future pregnancy intention.
Green Extraction of Annatto Seed Oily Extract and Its Use as a Pharmaceutical Material for the Production of Lipid Nanoparticles
Authors: Oliveira Sônia do Socorro do C. , Sarmento Edmilson dos S. , Marinho Victor H. , Pereira Rayanne R. , Fonseca Luis P. , Ferreira Irlon M.
Published: August 2022

This work developd nanomaterials formulated from annatto seed oily extract (ASE), myristic acid (tetradecanoic acid), and their fatty acid esters. The annatto seed oily extract was obtained using only soybean oil (ASE + SO) and Brazil nut oil (ASE + BNO). The UV/VIS analysis of the oily extracts showed three characteristic peaks of the bixin molecule at 430, 456 and 486 nm. The lipid nanoparticles obtained using myristic acid and ASE + BNO or only BNO showed better results than the oil soybean extract, i.e., the particle size was <200 nm, PDI value was in the range of 0.2–0.3, and had no visual physical instability as they kept stable for 28 days at 4 °C. Lipid nanoemulsions were also produced with esters of myristic acid and ASE + BNO. These fatty acid esters significantly influenced the particle size of nanoemulsions. For instance, methyl tetradecanoate led to the smallest particle size nanoemulsions (124 nm), homogeneous size distribution, and high physical stability under 4 and 32 °C for 28 days. This work demonstrates that the chemical composition of vegetable oils and myristic acid esters, the storage temperature, the chain length of fatty acid esters (FAE), and their use as co-lipids improve the physical stability of lipid nanoemulsions and nanoparticles from annatto seed oily extract.
Isorhamnetin Suppresses Human Gastric Cancer Cell Proliferation through Mitochondria-Dependent Apoptosis
Authors: Li Yehua , Fan Baoqiang , Pu Ning , Ran Xue , Lian Tiancheng , Cai Yifan , Xing Wei , Sun Kun
Published: August 2022

Derivates of natural products have been wildly utilized in the treatment of malignant tumors. Isorhamnetin (ISO), a most important active ingredient derived from flavonoids, shows great potential in tumor therapy. However, the therapeutic effects of ISO on gastric cancer (GC) remain unclear. Here, we demonstrate that ISO treatment dramatically inhibited the proliferation of two types of GC cells (AGS-1 and HGC-27) both in vitro and in vivo in time- and dose-dependent manners. These results are consistent with the transcriptomic analysis of ISO-treated GC cells, which yielded hundreds of differentially expressed genes that were enriched with cell growth and apoptosis. Mechanically, ISO treatment initiated the activation of caspase-3 cascade and elevated the expression of mitochondria-associated Bax/Bcl-2, cytosolic cytochrome c, followed by the activation of the cleavage of caspase-3 as well as poly ADP-ribose polymerase (PARP), resulting in the severe reduction of the mitochondrial potential and the accumulation of reactive oxygen species (ROS), while pre-treatment of the caspase-3 inhibitor could block the anti-tumor effect. Therefore, these results indicate that ISO treatment induces the apoptosis of GC cells through the mitochondria-dependent apoptotic pathway, providing a potential strategy for clinical GC therapy.