bluuebunny/crossref_metadata_2025 huggingface dataset:
Primary Health Care System Strengthening Project in Sri Lanka: Status and Challenges with Human Resources, Information Systems, Drugs and Laboratory Services
Authors: Thekkur Pruthu , Fernando Manoj , Nair Divya , Kumar Ajay M. V. , Satyanarayana Srinath , Chandraratne Nadeeka , Chandrasiri Amila , Attygalle Deepika Eranjanie , Higashi Hideki , Bandara Jayasundara , Berger Selma Dar , Harries Anthony D.
Published: November 2022

A Primary Healthcare-System-Strengthening Project (PSSP) is implemented by the Ministry of Health, Sri Lanka, with funding support from the World Bank for providing quality care through primary medical care institutions (PMCIs). We used an explanatory mixed-methods study to assess progress and challenges in human resources, drug availability, laboratory services and the health management information system (HMIS) at PMCIs. We conducted a checklist-based assessment followed by in-depth interviews of healthcare workers in one PMCI each in all nine provinces. All PMCIs had medical/nursing officers, but data entry operators (44%) and laboratory technicians (33%) were mostly not available. Existing staff were assigned additional responsibilities in PSSP, decreasing their motivation and efficiency. While 11/18 (61%) essential drugs were available in all PMCIs, buffer stocks were not maintained in >50% due to poor supply chain management and storage infrastructure. Only 6/14 (43%) essential laboratory investigations were available in >50% of PMCIs, non-availability was due to shortages of reagents/consumables and lack of sample collection–transportation system. The HMIS was installed in PMCIs but its usage was sub-optimal due to perceived lack of utility, few trained operators and poor internet connectivity. The PSSP needs to address these bottlenecks as a priority to ensure sustainability and successful scale-up.
Male Partners’ Roles in Infant Feeding Practices: Perspectives of Black Mothers Living with HIV in Three Countries
Authors: Etowa Josephine , Nare Hilary , Dubula-Majola Vuyiseka , Edet Olaide , John Mildred , Nkwocha Chioma Rose , Stephens Colleen , Mbewu Nokwanele , Hannan Jean , Etowa Egbe
Published: November 2022

Currently, mothers living with HIV (LWH) are challenged with different infant feeding guidelines depending on the country they are living in. This may contribute to confusion, stress, and mental health issues related to decision-making about infant feeding as a mother LWH. Yet, their male partners as their closest social capital have important roles to play in reducing or aggravating this psychosocial distress. Hence, we describe the role of male partners in supporting mothers who are living with HIV in the context of infant feeding. It is based on the results of a recent study of the socio-cultural context of infant feeding among Black mothers LWH in three countries; Canada, the USA, and Nigeria. The study was a tri-national, mixed-methods, community-based participatory research (CBPR) project, informed by postcolonialism and intersectionality theories. This paper is based on the qualitative component of the study. It was a focused ethnography (FE) involving 61 in-depth individual interviews (IDIs) with Black- mothers LWH. Thematic analysis guided the interpretation of these data, and trustworthiness was established through member-checking. Black mothers LWH acknowledged the various support roles that their male partners play in easing the practical and emotional burdens of infant feeding in the context of HIV. Male partners’ roles were captured under three sub-themes: (1) Practical help, (2) Protection of the family, and (3) Emotional support and sounding board. These findings have explicated the evolving ways in which male partners support ACB mothers LWH to promote positive infant feeding outcomes, as well as enhance the emotional and physical well-being of both mother and infant. Our study has explicated the evolving ways in which male partners support Black mothers LWH to promote positive infant feeding outcomes, as well as enhance the emotional and physical well-being of both mother and infant.
Efficacy of Home Oral-Hygiene Protocols during Orthodontic Treatment with Multibrackets and Clear Aligners: Microbiological Analysis with Phase-Contrast Microscope
Authors: Caccianiga Paolo , Nota Alessandro , Tecco Simona , Ceraulo Saverio , Caccianiga Gianluigi
Published: November 2022

The purpose of this study is to analyze the microbiota of patients undergoing orthodontic treatment with multibrackets and transparent aligners. The second goal is to evaluate the effectiveness of the oral irrigator on the oral hygiene and periodontal health of orthodontic patients. Fifty patients (27 F, 23 M; mean age 21.5 years) were recruited for the study, then divided into two groups. Group A underwent fixed orthodontic therapy with multibracket, and a home protocol that included manual orthodontic toothbrush, interdental brushes, and one-tuft brushes. Group B used transparent aligners for 22 h a day and a home protocol that included a manual brush with soft bristles and dental floss. After 3 months, all patients of the two groups, A and B, underwent plaque evaluation with a phase-contrast microscope. If the test result showed non-pathogenic bacterial flora, the subject continued with the traditional home oral-hygiene protocol. If the test detected pathogenic flora, the subject changed the home protocol, with a sonic toothbrush and oral irrigator, while the microbiological analysis continued to be performed after 3 months. After 3 months, 10 out of 25 patients treated with multibrackets (group A) and only 3 out of 25 patients with aligners (group B) passed from non-pathogenic flora to pathogenic flora. After 6 months, using the oral irrigator and a sonic toothbrush for 3 months, all subjects returned to non-pathogenic flora. This study confirms that in patients treated with multibrackets, the risk of developing unfavorable microbiota increases compared to those treated with clear aligners. The use of an oral irrigator combined with the sonic toothbrush seems to be able to restore good oral hygiene in subjects with pathogenic flora and therefore to be effective at reducing the risk of caries and gingivitis in orthodontic patients.
Analysis of the Anaerobic Power Output, Dynamic Stability, Lower Limb Strength, and Power of Elite Soccer Players Based on Their Field Position
Authors: AlTaweel Ali , Nuhmani Shibili , Ahsan Mohammad , Al Muslem Wafa Hashem , Abualait Turki , Muaidi Qassim Ibrahim
Published: November 2022

Soccer players require a high degree of aerobic and anaerobic fitness to perform well throughout the game as per their position in the field. This study aimed to investigate the differences in anaerobic power output, dynamic stability, lower limb strength, and power among elite soccer players based on their field position. A cross-sectional population of 96 elite soccer players with average age 23.10 ± 4.35 years, weight 69.99 ± 9.71 kg, height 174.84 ± 6.64 cm, and body mass index 22.84 ± 2.39 kg/m2 from various soccer clubs in Saudi Arabia was tested for their anaerobic power output, dynamic stability, lower limb strength, and power performance. All the participants have more than 4 years of experience in competitive soccer events. Tests included a measure of single-leg vertical jump, star excursion balance test, and single-leg triple hop test for distance. The players were divided into four groups (goalkeepers, defenders, midfielders, and attackers) based on their self-reported position on the field. One-way ANOVA was used to determine the differences between all variables according to the players’ position. In addition, partial eta-squared (ηp2) was used to report effect sizes. The results revealed significant differences between positions in the anaerobic power output (p = 0.012, ηp2 = 0.312), dynamic stability {Anterior (p = 0.004, ηp2 = 0.235), Anteromedial (p = 0.007, ηp2 = 0.622), Anterolateral (p = 0.011, ηp2 = 0.114)}, and lower limb strength, and power (p = 0.008, ηp2 = 0.421). At the same time, goalkeepers’ performance was significantly superior to midfielders (p = 0.006) in the anaerobic power output. In addition, lower limb strength and power was significantly higher (p = 0.004) for goalkeepers than for midfielders, with a similar trend in dynamic stability (p = 0.007). These results exhibited differences in anaerobic power output, dynamic stability, lower limb strength, and power performance based on the players’ positions. The investigation may assist the practitioner in designing training programs for the players according to their position for performance improvement.
How to Accommodate the Emotional Dimensions of Advance Care Planning Using Motivational Interviewing and Conditional Medical Orders
Authors: Stuart Richard B. , Birchfield George , Thielke Stephen
Published: November 2022

Palliative care discussions offer a unique opportunity for helping patients choose end-of-life (EOL) treatments. These are among the most difficult decisions in later life, and protecting patients’ ability to make these choices is one of healthcare’s strongest ethical mandates. Yet, traditional approaches to advance care planning (ACP) have only been moderately successful in helping patients make decisions that lead to treatments concordant with their values. In particular, neglect of attention to the emotions that occur during consideration of the end of one’s life contributes to patients’ difficulty with engaging in the process and following through on decisions. To improve ACP outcomes, providers can address the patient’s emotional experiences, and can use motivational interviewing as a way attend to elicit them and incorporate them into care planning. Applying personalizing emotion-attuned protocols like Conditional Medical Orders (CMO) also promotes this end.
Change and Continuity in Preventive Practices across the COVID-19 Pandemic among Rural and Urban Latinx Immigrant Worker Families
Authors: Quandt Sara A. , Smith Sydney A. , Talton Jennifer W. , Chen Haiying , Laurienti Paul J. , Arcury Thomas A.
Published: November 2022

(1) Background: The COVID-19 pandemic has put essential workers at high risk for contracting the disease. This study documents situational compliance with public health recommendations such as masking and social distancing among rural and urban Latinx families, with the goal of understanding change over time in COVID-19 risk reduction behaviors. (2) Methods: Respondents for 67 rural families and 44 urban families responded to repeated telephone surveys at three time points in the first year of the pandemic, providing data on use of masks and social distancing by themselves and family members while interacting with others at home, work, and in the community. Cumulative logistic regression models were employed to compare changes in risk behaviors between rural and urban groups over time. (3) Results: While group descriptive results indicated behaviors that posed low risk at each time point, regression models revealed greater change between time points for rural than urban residents. Rural residents also had gendered patterns. (4) Conclusions: Patterns of change appear to reflect structural issues such as seasonal labor demand and gender roles more than pandemic fatigue or changing public health recommendations. The findings suggest that structural factors play a role in individuals complying with public health prevention measures for COVID-19.
Mammalian Target of Rapamycin (mTOR) Signaling at the Crossroad of Muscle Fiber Fate in Sarcopenia
Authors: Sirago Giuseppe , Picca Anna , Calvani Riccardo , Coelho-Júnior Hélio José , Marzetti Emanuele
Published: November 2022

The mammalian target of rapamycin (mTOR) is a major regulator of skeletal myocyte viability. The signaling pathways triggered by mTOR vary according to the type of endogenous and exogenous factors (e.g., redox balance, nutrient availability, physical activity) as well as organismal age. Here, we provide an overview of mTOR signaling in skeletal muscle, with a special focus on the role played by mTOR in the development of sarcopenia. Intervention strategies targeting mTOR in sarcopenia (e.g., supplementation of plant extracts, hormones, inorganic ions, calorie restriction, and exercise) have also been discussed.
Urinary Measurement of Epigenetic DNA Modifications and 8-oxodG as Possible Noninvasive Markers of Colon Cancer Evolution
Authors: Skalska-Bugala Aleksandra , Siomek-Gorecka Agnieszka , Banaszkiewicz Zbigniew , Olinski Ryszard , Rozalski Rafal
Published: November 2022

The active DNA demethylation mechanism involves 5-methylcytosine (5-mCyt) enzymatic oxidation with the subsequent formation of 5-hydroxymethylcytosine (5-hmCyt), which can be further oxidized to 5-formylcytosine (5-fCyt) and 5-carboxylcytosine (5-caCyt). The products of active DNA demethylation are released into the bloodstream and eventually also appear in urine. We used online two-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS) to compare DNA methylation marks and 8-oxo-2′-deoxyguanosine (8-oxodG) in colorectal cancer and pre-cancerous condition in urine. The study included four groups of subjects: healthy controls, patients with inflammatory bowel disease (IBD), persons with adenomatous polyps (AD), and individuals with colorectal cancer (CRC). We have found that the level of 5-fCyt in urine was significantly lower for CRC and polyp groups than in the control group. The level of 5-hmCyt was significantly higher only in the CRC group compared to the control (2.3 vs. 2.1 nmol/mmol creatinine). Interestingly, we have found highly statistically significant correlation of 5-hydroxymethyluracil with 5-hydroxymethylcytosine, 5-(hydroxymethyl)-2′-deoxycytidine, 5-(hydroxymethyl)-2′-deoxyuridine, and 5-methyl-2′-deoxycytidine in the CRC patients’ group.
Impact of JH Signaling on Reproductive Physiology of the Classical Insect Model, Rhodnius prolixus
Authors: Leyria Jimena , Orchard Ian , Lange Angela B.
Published: November 2022

In adult females of several insect species, juvenile hormones (JHs) act as gonadotrophic hormones, regulating egg production. JH binds to its nuclear receptor, Methoprene tolerant (Met), triggering its dimerization with the protein Taiman (Tai). The resulting active complex induces transcription of JH response genes, such as Krüppel homolog 1 (Kr-h1). In this study we report for the first time the participation of the isoform JH III skipped bisepoxide (JHSB3) and its signaling pathway in the reproductive fitness of the classical insect model Rhodnius prolixus. The topical application of synthetic JHSB3 increases transcript and protein expression of yolk protein precursors (YPPs), mainly by the fat body but also by the ovaries, the second source of YPPs. These results are also confirmed by ex vivo assays. In contrast, when the JH signaling cascade is impaired via RNA interference by downregulating RhoprMet and RhoprTai mRNA, egg production is inhibited. Although RhoprKr-h1 transcript expression is highly dependent on JHSB3 signaling, it is not involved in egg production but rather in successful hatching. This research contributes missing pieces of JH action in the insect model in which JH was first postulated almost 100 years ago.
Computer Aided Diagnosis of Melanoma Using Deep Neural Networks and Game Theory: Application on Dermoscopic Images of Skin Lesions
Authors: Foahom Gouabou Arthur Cartel , Collenne Jules , Monnier Jilliana , Iguernaissi Rabah , Damoiseaux Jean-Luc , Moudafi Abdellatif , Merad Djamal
Published: November 2022

Early detection of melanoma remains a daily challenge due to the increasing number of cases and the lack of dermatologists. Thus, AI-assisted diagnosis is considered as a possible solution for this issue. Despite the great advances brought by deep learning and especially convolutional neural networks (CNNs), computer-aided diagnosis (CAD) systems are still not used in clinical practice. This may be explained by the dermatologist’s fear of being misled by a false negative and the assimilation of CNNs to a “black box”, making their decision process difficult to understand by a non-expert. Decision theory, especially game theory, is a potential solution as it focuses on identifying the best decision option that maximizes the decision-maker’s expected utility. This study presents a new framework for automated melanoma diagnosis. Pursuing the goal of improving the performance of existing systems, our approach also attempts to bring more transparency in the decision process. The proposed framework includes a multi-class CNN and six binary CNNs assimilated to players. The players’ strategies is to first cluster the pigmented lesions (melanoma, nevus, and benign keratosis), using the introduced method of evaluating the confidence of the predictions, into confidence level (confident, medium, uncertain). Then, a subset of players has the strategy to refine the diagnosis for difficult lesions with medium and uncertain prediction. We used EfficientNetB5 as the backbone of our networks and evaluated our approach on the public ISIC dataset consisting of 8917 lesions: melanoma (1113), nevi (6705) and benign keratosis (1099). The proposed framework achieved an area under the receiver operating curve (AUROC) of 0.93 for melanoma, 0.96 for nevus and 0.97 for benign keratosis. Furthermore, our approach outperformed existing methods in this task, improving the balanced accuracy (BACC) of the best compared method from 77% to 86%. These results suggest that our framework provides an effective and explainable decision-making strategy. This approach could help dermatologists in their clinical practice for patients with atypical and difficult-to-diagnose pigmented lesions. We also believe that our system could serve as a didactic tool for less experienced dermatologists.
Spontaneous Transformation of a p53 and Rb-Defective Human Fallopian Tube Epithelial Cell Line after Long Passage with Features of High-Grade Serous Carcinoma
Authors: Chang Yu-Hsun , Chu Tang-Yuan , Ding Dah-Ching
Published: November 2022

Ovarian cancer is one of the most lethal gynecological cancers, and 80% are high-grade serous carcinomas (HGSOC). Despite advances in chemotherapy and the development of targeted therapies, the survival rate of HGSOC has only moderately improved. Therefore, a cell model that reflects the pathogenesis and clinical characteristics of this disease is urgently needed. We previously developed a human fallopian tube epithelial cell line (FE25) with p53 and Rb deficiencies. After long-term culture in vitro, cells at high-passage numbers showed spontaneous transformation (FE25L). This study aimed to compare FE25 cells cultured in vitro for low (passage 16–31) and high passages (passage 116–139) to determine whether these cells can serve as an ideal cell model of HGSOC. Compared to the cells at low passage, FE25L cells showed increased cell proliferation, clonogenicity, polyploidy, aneuploidy, cell migration, and invasion. They also showed more resistance to chemotherapy and the ability to grow tumors in xenografts. RNA-seq data also showed upregulation of hypoxia, epithelial-mesenchymal transition (EMT), and the NF-κB pathway in FE25L compared to FE25 cells. qRT-PCR confirmed the upregulation of EMT, cytokines, NF-κB, c-Myc, and the Wnt/β-catenin pathway. Cross-platform comparability found that FE25L cells could be grouped with the other most likely HGSOC lines, such as TYKNU and COV362. In conclusion, FE25L cells showed more aggressive malignant behavior than FE25 cells and hence might serve as a more suitable model for HGSOC research.
Sesamolin Alleviates Nonalcoholic Fatty Liver Disease through Modulating Gut Microbiota and Metabolites in High-Fat and High-Fructose Diet-Fed Mice
Authors: Yu Jing , Sun Hao , Yang Yang , Yan Yaping
Published: November 2022

Nonalcoholic fatty liver disease (NAFLD) has become a major public health problem. The effects of sesamolin on obesity-associated NAFLD and its possible mechanism are still poorly understood. The present study investigated the effects of sesamolin on NAFLD and changes in gut microbiota and serum metabolites in high-fat and high-fructose (HF-HF) diet-fed mice. Mice with NAFLD were treated with or without sesamolin. Sesamolin effectively suppressed obesity-associated metabolic disorder, attenuated hepatic steatosis and the infiltration of inflammatory cells, and decreased levels of hepatic proinflammatory cytokines. Sesamolin also altered the composition of gut microbiota at the genus level. Additionally, differential serum metabolite biomarkers identified in an untargeted metabolomics analysis showed that sesamolin changed the levels of metabolites and influenced metabolomics pathways including caffeine metabolism, steroid hormone biosynthesis, and cysteine and methionine metabolism. Changes in metabolite biomarkers and the abundances of Faecalibaculum, Lachnoclostridium, Mucispirillum, Allobaculum, and Bacteroides are highly correlated with those factors involved in the progression of NAFLD. These results are important in deciphering new mechanisms by which changes in bacteria and metabolites in sesamolin treatment might be associated with the alleviation of obesity-associated NAFLD in HF-HF diet-fed mice. Thus, sesamolin may be a potential compound for obesity-associated NAFLD treatment.
Systematic Identification and Expression Analysis of the Sorghum Pht1 Gene Family Reveals Several New Members Encoding High-Affinity Phosphate Transporters
Authors: Zhang Jinglong , Shen Yixin , Chen Wei , Bai Binqiang , Ji Xiaomin , Chi Yingjun
Published: November 2022

Sorghum (Sorghum bicolor) is known to have a more robust capability of phosphorus uptake than many other cereal plants, which could be attributed to its phosphate transporter 1 (Pht1) that has a high phosphorus affinity. There are eleven SbPht1 genes in the sorghum genome, nine of which are expressed in sorghum roots or shoots in response to phosphorus deficiency (low-P). The molecular features of these nine genes were investigated by gene expression analysis, subcellular localization, and a yeast mutant complementation growth assay. They were found to be induced in response to low-P stress in root or shoot. All these SbPht1 proteins were found to be localized on the cell membrane, and SbPht1;8 was also detected in the endoplasmic reticulum. These SbPht1s were able to complement the yeast mutant EY917 that lacks all the functional phosphate transporters, and, among them, SbPht1;5, SbPht1;6 and SbPht1;8 could partially complement the yeast mutant strain EY917 in low-P conditions. Overall, these findings demonstrate that SbPht1;5, SbPht1;6, and SbPht1;8 are high-affinity phosphate transporters. SbPht1;5, in particular, is specifically involved in phosphorus uptake in the roots, whilst SbPht1;6 and SbPht1;8 are key players in both P uptake and P transport in response to low-P stress in sorghum.
Label-Free Surface-Enhanced Raman Spectroscopic Analysis of Proteins: Advances and Applications
Authors: Cai Linjun , Fang Guilin , Tang Jinpin , Cheng Qiaomei , Han Xiaoxia
Published: November 2022

Surface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS is useful for probing intrinsic structural information of proteins and is attracting increasing attention in biophysics, bioanalytical chemistry, and biomedicine. This review starts with a brief introduction of SERS theories and SERS methodology of protein structural characterization. SERS-active materials, related synthetic approaches, and strategies for protein-material assemblies are outlined and discussed, followed by detailed discussion of SERS spectroscopy of proteins with and without cofactors. Recent applications and advances of protein SERS in biomarker detection, cell analysis, and pathogen discrimination are then highlighted, and the spectral reproducibility and limitations are critically discussed. The review ends with a conclusion and a discussion of current challenges and perspectives of promising directions.
Lignin Microspheres Modified with Magnetite Nanoparticles as a Selenate Highly Porous Adsorbent
Authors: Marjanovic Vesna , Markovic Radmila , Steharnik Mirjana , Dimitrijevic Silvana , Marinkovic Aleksandar D. , Peric-Grujic Aleksandra , Đolic Maja
Published: November 2022

Highly porous lignin-based microspheres, modified by magnetite nanoparticles, were used for the first time for the removal of selenate anions, Se(VI), from spiked and real water samples. The influence of experimental conditions: selenate concentration, adsorbent dosage and contact time on the adsorption capacity was investigated in a batch experimental mode. The FTIR, XRD, SEM techniques were used to analyze the structural and morphological properties of the native and exhausted adsorbent. The maximum adsorption capacity was found to be 69.9 mg/g for Se(VI) anions at pH 6.46 from the simulated water samples. The modified natural polymer was efficient in Se(VI) removal from the real (potable) water samples, originated from six cities in the Republic of Serbia, with an overage efficacy of 20%. The regeneration capacity of 61% in one cycle of desorption (0.5 M NaOH as desorption solution) of bio-based adsorbent was gained in this investigation. The examined material demonstrated a significant affinity for Se(VI) oxyanion, but a low potential for multi-cycle material application; consequently, the loaded sorbent could be proposed to be used as a Se fertilizer.
Dose-Dependent Cytotoxicity of Polypropylene Microplastics (PP-MPs) in Two Freshwater Fishes
Authors: Bobori Dimitra C. , Feidantsis Konstantinos , Dimitriadi Anastasia , Datsi Nefeli , Ripis Panagiotis , Kalogiannis Stavros , Sampsonidis Ioannis , Kastrinaki Georgia , Ainali Nina Maria , Lambropoulou Dimitra A. , Kyzas George Z. , Koumoundouros George , Bikiaris Dimitrios N. , Kaloyianni Martha
Published: November 2022

The massive accumulation of plastics over the decades in the aquatic environment has led to the dispersion of plastic components in aquatic ecosystems, invading the food webs. Plastics fragmented into microplastics can be bioaccumulated by fishes via different exposure routes, causing several adverse effects. In the present study, the dose-dependent cytotoxicity of 8–10 μm polypropylene microplastics (PP-MPs), at concentrations of 1 mg/g (low dose) and 10 mg/g dry food (high dose), was evaluated in the liver and gill tissues of two fish species, the zebrafish (Danio rerio) and the freshwater perch (Perca fluviatilis). According to our results, the inclusion of PP-MPs in the feed of D. rerio and P. fluviatilis hampered the cellular function of the gills and hepatic cells by lipid peroxidation, DNA damage, protein ubiquitination, apoptosis, autophagy, and changes in metabolite concentration, providing evidence that the toxicity of PP-MPs is dose dependent. With regard to the individual assays tested in the present study, the biggest impact was observed in DNA damage, which exhibited a maximum increase of 18.34-fold in the liver of D. rerio. The sensitivity of the two fish species studied differed, while no clear tissue specificity in both fish species was observed. The metabolome of both tissues was altered in both treatments, while tryptophan and nicotinic acid exhibited the greatest decrease among all metabolites in all treatments in comparison to the control. The battery of biomarkers used in the present study as well as metabolomic changes could be suggested as early-warning signals for the assessment of the aquatic environment quality against MPs. In addition, our results contribute to the elucidation of the mechanism induced by nanomaterials on tissues of aquatic organisms, since comprehending the magnitude of their impact on aquatic ecosystems is of great importance.
Arsenic Induces M2 Macrophage Polarization and Shifts M1/M2 Cytokine Production via Mitophagy
Authors: Hung Chih-Hsing , Hsu Hua-Yu , Chiou Hsin-Ying Clair , Tsai Mei-Lan , You Huey-Ling , Lin Yu-Chih , Liao Wei-Ting , Lin Yi-Ching
Published: November 2022

Arsenic is an environmental factor associated with epithelial–mesenchymal transition (EMT). Since macrophages play a crucial role in regulating EMT, we studied the effects of arsenic on macrophage polarization. We first determined the arsenic concentrations to be used by cell viability assays in conjunction with previous studies. In our results, arsenic treatment increased the alternatively activated (M2) macrophage markers, including arginase 1 (ARG-1) gene expression, chemokine (C-C motif) ligand 16 (CCL16), transforming growth factor-β1 (TGF-β1), and the cluster of differentiation 206 (CD206) surface marker. Arsenic-treated macrophages promoted A549 lung epithelial cell invasion and migration in a cell co-culture model and a 3D gel cell co-culture model, confirming that arsenic treatment promoted EMT in lung epithelial cells. We confirmed that arsenic induced autophagy/mitophagy by microtubule-associated protein 1 light-chain 3-II (LC3 II) and phosphor-Parkin (p-Parkin) protein markers. The autophagy inhibitor chloroquine (CQ) recovered the expression of the inducible nitric oxide synthase (iNOS) gene in arsenic-treated M1 macrophages, which represents a confirmation that arsenic indeed induced the repolarization of classically activated (M1) macrophage to M2 macrophages through the autophagy/mitophagy pathway. Next, we verified that arsenic increased M2 cell markers in mouse blood and lungs. This study suggests that mitophagy is involved in the arsenic-induced M1 macrophage switch to an M2-like phenotype.
Transcription Factors as Targets of Natural Compounds in Age-Related Diseases and Cancer: Potential Therapeutic Applications
Authors: Kim Mi Eun , Kim Dae Hyun , Lee Jun Sik
Published: November 2022

Inflammation exacerbates systemic pathophysiological conditions and chronic inflammation is a sustained and systemic phenomenon that aggravates aging that can lead to chronic age-related diseases. These inflammatory phenomena have recently been redefined and delineated at the molecular, cellular, and systemic levels. Many transcription factors that are activated in response to tumor metabolic state have been reported to be regulated by a class of histone deacetylase called sirtuins (SIRTs). Sirtuins play a pivotal role in the regulation of tumor cell metabolism, proliferation, and angiogenesis, including oxidative stress and inflammation. The SIRT1-mediated signaling pathway in diabetes and cancer is the SIRT1/forkhead-box class O (FoxO)/nuclear factor-kappa B (NF-κB) pathway. In this review, we describe the accumulation of SIRT1-, NF-κB-, and FoxO-mediated inflammatory processes and cellular proinflammatory signaling pathways. We also describe the proinflammatory mechanisms underlying metabolic molecular pathways in various diseases such as liver cancer and diabetes. Finally, the regulation of cancer and diabetes through the anti-inflammatory effects of natural compounds is highlighted. Evidence from inflammation studies strongly suggests that cells may be a major source of cytokines secreted during various diseases. A better understanding of the mechanisms that underpin the inflammatory response and palliative role of natural compounds will provide insights into the molecular mechanisms of inflammation and various diseases for potential intervention.
Recent Insights into Neutrophil Extracellular Traps in Cardiovascular Diseases
Authors: Dong Yuan , Zhang Yuejie , Yang Xuanyi , Yan Cen , Feng Yingmei
Published: November 2022

Neutrophils are primary effector cells of the innate immune system. Emerging evidence has consistently shown that activated neutrophils produce and release neutrophil extracellular traps (NETs) that play roles in immunity and non-infectious diseases. NETs are composed of DNA and proteins and serve as a structural platform for pathogen sequestration and degradation. In contrast to their protective role during pathogenic infection, NETs are pathologically involved in cardiovascular disease (CVD). In this review, we introduce the formation, release, and clearance of NETs and the regulatory mechanisms of NETs formation, followed by an overview of the clinical evidence for the involvement of NETs in CVD. Because atherosclerosis is a fundamental part of the pathogenesis of CVD, we chose to focus on the mechanisms by which NETs promote endothelial cell damage and collaborate with macrophages and platelets to accelerate plaque progression and thrombosis. Finally, we present options for clinical intervention to inhibit NETs production and release in the treatment of CVD. In conclusion, this review integrates the latest findings and provides new insights into NETs, which represent a novel biomarker and therapeutic target in clinical practice.
Innate Immune Cells during Machine Perfusion of Liver Grafts—The Janus Face of Hepatic Macrophages
Authors: Roushansarai Nicola Sariye , Pascher Andreas , Becker Felix
Published: November 2022

Machine perfusion is an emerging technology in the field of liver transplantation. While machine perfusion has now been implemented in clinical routine throughout transplant centers around the world, a debate has arisen regarding its concurrent effect on the complex hepatic immune system during perfusion. Currently, our understanding of the perfusion-elicited processes involving innate immune cells remains incomplete. Hepatic macrophages (Kupffer cells) represent a special subset of hepatic immune cells with a dual pro-inflammatory, as well as a pro-resolving and anti-inflammatory, role in the sequence of ischemia–reperfusion injury. The purpose of this review is to provide an overview of the current data regarding the immunomodulatory role of machine perfusion and to emphasize the importance of macrophages for hepatic ischemia–reperfusion injury.
Pulmonary Arterial Hypertension in Genetic and Comorbid Settings: A Step Forward for Precision Medicine
Authors: Madonna Rosalinda
Published: November 2022

The editorial refers to the Special Issue “Pulmonary Arterial Hypertension: Old Drugs and New Treatment Strategies” [...]
Robotics in Total Hip Arthroplasty: Current Concepts
Authors: Bullock Emily , Brown Michael , Clark Gavin , Plant James , Blakeney William
Published: November 2022

This current concepts article reviews the literature pertaining to the use of robot-assisted systems in total hip arthroplasty (THA). The bulk of the literature is regarding the MAKO (currently the most used system worldwide) and the historic ROBODOC robotic systems. There is a paucity of literature available on other systems, with several still in pilot-phase development. Whilst the evidence shows improved radiological outcomes with robotic THA, functional outcomes are equivocal between conventional and robotic techniques. Acceptance of robotic THA worldwide is limited by its accessibility including cost, and by already exceptional results with the conventional technique. It is, however, a rapidly developing area of orthopaedic surgery. This article discusses the history of robotics in THA, current surgical techniques, functional and radiological outcomes, and ongoing avenues for development.
Clinical Presentations and Multimodal Imaging Diagnosis in Chronic Thromboembolic Pulmonary Hypertension
Authors: Jung Mi-Hyang , Jung Hae Ok , Kwon Soo Jin , Chang Suyon
Published: November 2022

Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare but life-threatening pulmonary vascular disease caused by the presence of a prolonged thrombus in the pulmonary artery. CTEPH is a distinct disease entity classified as group 4 pulmonary hypertension according to the World Symposium on Pulmonary Hypertension. It is the only potentially curable cause of pulmonary hypertension. However, timely diagnosis and treatment are often hampered by nonspecific symptoms and signs and a lack of physician awareness regarding the condition. Thus, it is important to be familiar with the clinical features of CTEPH and the associated diagnostic processes. Herein, we cover the diagnostic approach for CTEPH using multimodal imaging tools in a clinical setting.
3D Pose Estimation and Tracking in Handball Actions Using a Monocular Camera
Authors: Šajina Romeo , Ivašić-Kos Marina
Published: November 2022

Player pose estimation is particularly important for sports because it provides more accurate monitoring of athlete movements and performance, recognition of player actions, analysis of techniques, and evaluation of action execution accuracy. All of these tasks are extremely demanding and challenging in sports that involve rapid movements of athletes with inconsistent speed and position changes, at varying distances from the camera with frequent occlusions, especially in team sports when there are more players on the field. A prerequisite for recognizing the player’s actions on the video footage and comparing their poses during the execution of an action is the detection of the player’s pose in each element of an action or technique. First, a 2D pose of the player is determined in each video frame, and converted into a 3D pose, then using the tracking method all the player poses are grouped into a sequence to construct a series of elements of a particular action. Considering that action recognition and comparison depend significantly on the accuracy of the methods used to estimate and track player pose in real-world conditions, the paper provides an overview and analysis of the methods that can be used for player pose estimation and tracking using a monocular camera, along with evaluation metrics on the example of handball scenarios. We have evaluated the applicability and robustness of 12 selected 2-stage deep learning methods for 3D pose estimation on a public and a custom dataset of handball jump shots for which they have not been trained and where never-before-seen poses may occur. Furthermore, this paper proposes methods for retargeting and smoothing the 3D sequence of poses that have experimentally shown a performance improvement for all tested models. Additionally, we evaluated the applicability and robustness of five state-of-the-art tracking methods on a public and a custom dataset of a handball training recorded with a monocular camera. The paper ends with a discussion apostrophizing the shortcomings of the pose estimation and tracking methods, reflected in the problems of locating key skeletal points and generating poses that do not follow possible human structures, which consequently reduces the overall accuracy of action recognition.
Microbial Biofilms Colonizing Plastic Substrates in the Ross Sea (Antarctica)
Authors: Caroppo Carmela , Azzaro Maurizio , Dell’Acqua Ombretta , Azzaro Filippo , Maimone Giovanna , Rappazzo Alessandro Ciro , Raffa Francesco , Caruso Gabriella
Published: November 2022

Very few studies have investigated marine microbial colonization in polar regions, but climate-changing scenarios stress the importance of these investigations to protect life in such extremely vulnerable ecosystems. In two different coastal sites of the Ross Sea (Road and Tethys Bays, Antarctica) exposed to different stressors, the microbial biofilm colonizing the surface of plastic (polyvinyl chloride, PVC, and polyethylene, PE) panels left submerged in two experiments at different timescales (“short-term”: 3 months, and “long-term”: 9 and 12 months) was studied. The abundance and metabolic enzymatic activities [leucine aminopeptidase (LAP), beta-glucosidase (GLU) and alkaline phosphatase (AP)] of the prokaryotes and the microalgal abundance and species composition were analyzed, in parallel with the main environmental parameters. The prokaryotic community showed higher abundance and metabolic activities on PVC than on PE as opposed to microalgae. A peak in the microfouling prokaryotic abundance and metabolic functions was frequently recorded after 3 months of immersion, corresponding to the late austral summer period. LAP and AP were the most active enzymes, suggesting that microbial metabolic profiles were modulated by labile organic substrates. Our results suggest that the composition and function of microbial biofilm could be considered as sentinels of natural or anthropic-related disturbances.
Assessing Coastal Flood Risk in a Changing Climate for Dublin, Ireland
Authors: Paranunzio Roberta , Guerrini Marco , Dwyer Edward , Alexander Paul J. , O’Dwyer Barry
Published: November 2022

With increasing urban expansion and population growth, coastal urban areas will be increasingly affected by climate change impacts such as extreme storm events, sea level rise and coastal flooding. To address coastal inundation risk for impact studies, integrated approaches accounting for flood hazard modelling, exposure and vulnerability of human and environmental systems are crucial. In this study, we model the impacts of sea level rise on coastal inundation depth for County Dublin, the most extensively urbanized area in Ireland, for the current period and for 2100 under two Representative Concentration Pathways RCP 4.5 and 8.5. A risk-centred approach has been considered by linking the information on coastal flood-prone areas to the exposure of the urban environment, in terms of potential future land cover changes, and to the socioeconomic vulnerability of the population. The results suggest significant challenges for Dublin city and the surrounding coastal areas, with an increase of around 26% and 67% in the number of administrative units considered at very high risk by the end of the century under a RCP 4.5 and 8.5, respectively. This study aims to contribute to existing coastal inundation research undertaken for Ireland by (i) providing a first-level screening of flooding hazards in the study area, (ii) demonstrating how land cover changes and socioeconomic vulnerability can contribute to the level of experienced risk and (iii) informing local authorities and at-risk communities so as to support them in the development of plans for adaptation and resilience.
Design of Combined Neural Network and Fuzzy Logic Controller for Marine Rescue Drone Trajectory-Tracking
Authors: Pham Duc-Anh , Han Seung-Hun
Published: November 2022

In recent years, the research on drones has increased rapidly because of its high applicability in many fields and its great development potential. In the maritime sector too, especially marine rescue, a Drone with a compact size and fast flight speed is an effective solution in search and surveillance, giving quick results and being very convenient. When operating at sea, marine rescue drones are often affected by the environment, especially wind, which leads to turbulence that causes the drone to deviate from its predetermined flight trajectory. To overcome the above problem, the author has proposed the application of a Neural-Fuzzy controller for unmanned marine rescue aircraft presented in this paper introduces a controller that combines neural networks and fuzzy controllers that enhance the efficiency of the drone’s trajectory tracking. The paper presents the mathematics of a quadcopter described by the Newton-Euler equations. Presentation on stable flight control and trajectory control of marine rescue drones. In this paper, Matlab/Simulink is used to describe the operation of the Drone, and the characteristics obtained after using the simulation are used to compare, test, and analyze the system. The obtained results show that the Neural-Fuzzy controller is much more sensitive, more resistant to turbulence, and can be used on different sizes, weights, and configurations of drones without adjusting PID gain.
Holistic Approach to Ship Design
Authors: Papanikolaou Apostolos D.
Published: November 2022

The recently completed Horizon 2020 European Research project—HOLISHIP—Holistic Optimization of Ship Design and Operation for Life Cycle (2016–2020) has developed suitable tools and software platforms which are necessary for the creation of innovative design solutions meeting the set low-emission strategic objectives. The present paper introduces an innovative, holistic approach to ship design and the development of integrated design software platforms and tools, which are used in practical applications. In the era of the 4th industrial revolution, this project sets out to substantially advance ship design via the introduction of a fully computerized, multi-disciplinary optimization approach to ship design and life-cycle operation. The approach enables the exploration of a huge design space in a relatively short time, as well as the distributed/multi-site working and the virtual reality testing; thus, it is a strong asset for the development of innovative maritime concepts in response to the needs of the 21st century.
Participatory Approach to Build Up a Municipal Strategy for Coastal Erosion Mitigation and Adaptation to Climate Change
Authors: Matos Fábio André , Alves Filipe , Coelho Carlos , Lima Márcia , Vizinho André
Published: November 2022

The Ovar coastline is characterized as one of the coastal areas with the highest vulnerability and risk of erosion in the Portuguese coast. The high-energy coast is further threatened by a permanent sediment deficit of anthropic origin, as well as sea level rise due to climate change. It is essential to define modern coastal adaptation strategies to minimize the impacts of these issues on the local communities, while considering social, environmental, and economic factors. It is in this territory that the INCCA project’s case study is located, involving stakeholders with technical, scientific, and operational knowledge in the co-management of the coastal stretch. In the scope of the involvement intended for the project’s development, five participatory events were held, involving local authorities, civil protection, public entities, academia, and the general community. These events allowed a multidisciplinary and multi-stakeholder analysis of the challenges and possible solutions to mitigate coastal erosion, representing the definition of a shared vision for the coastline’s future in this municipality. This work presents the main results of this participatory process as well as reflections on the importance of active citizenship instruments and stakeholder involvement for integrated coastal management in the 21st century.
Advances in Maritime Safety
Authors: Park Young-Soo , Jeong Jung-Sik , Aydogdu Yusuf Volkan
Published: November 2022

Due to the nature of business, maritime safety plays a paramount role in preventing damage to property and danger to life in the maritime industry [...]
New Insights into the Mechanism of Trichoderma virens-Induced Developmental Effects on Agrostis stolonifera Disease Resistance against Dollar Spot Infection
Authors: Gan Lu , Yin Yuelan , Niu Qichen , Yan Xuebing , Yin Shuxia
Published: November 2022

Trichoderma is internationally recognized as a biocontrol fungus for its broad-spectrum antimicrobial activity. Intriguingly, the crosstalk mechanism between the plant and Trichoderma is dynamic, depending on the Trichoderma strains and the plant species. In our previous study, the Trichoderma virens 192-45 strain showed better pathogen inhibition through the secretive non-volatile and volatile substrates. Therefore, we studied transcriptional and metabolic responses altered in creeping bentgrass (Agrostis stolonifera L.) with T. virens colonization prior to a challenge with Clarireedia homoeocarpa. This fungal pathogen causes dollar spot on various turfgrasses. When the pathogen is deficient, the importance of T. virens to the enhancement of plant growth can be seen in hormonal production and microbe signaling, such as indole-3-acrylic acid. Therefore, these substrates secreted by T. virens and induced genes related to plant growth can be the ‘pre-defense’ for ensuing pathogen attacks. During C. homoeocarpa infection, the Trichoderma–plant interaction activates defense responses through the SA- and/or JA-dependent pathway, induced by T. virens and its respective exudates, such as oleic, citric, and stearic acid. Thus, we will anticipate a combination of genetic engineering and exogenous application targeting these genes and metabolites, which could make creeping bentgrass more resistant to dollar spot and other pathogens.