bluuebunny/crossref_metadata_2025 huggingface dataset:
Comment on ``Superradiant stability of the Kerr black holes'' by Huang
Authors: CHEN WEN-XIANG
Published: February 2024

It has been claimed in \cite{1} that this research investigates the superradiant stability of a system comprising a Kerr black hole and a massive scalar perturbation. Previous studies have demonstrated superradiant stability under the condition $\mu \geq \sqrt{2} m \Omega_H$, where $\mu$ represents the scalar's proper mass, $m$ denotes the azimuthal number of the scalar mode, and $\Omega_H$ is the angular velocity of the Kerr black hole's horizon. Their work serves as a complement to these findings. They analytically establish that in the complementary parameter space, $\mu < \sqrt{2} m \Omega_H$, the system maintains superradiant stability if the scalar perturbation and Kerr black hole parameters meet two straightforward criteria: $\omega < \frac{\mu}{\sqrt{2}}$ and $\frac{r_{-}}{r_{+}} < 0.802$.The condition derived therein involves four variables (\(z_1\), \(z_2\), \(z_3\), and \(z_4\)) which are all negative. Given the focus of the cited article on analyzing the stability condition of \(B_1\), imposing constraints on the sign (positive or negative) of \(z_1\) and \(z_2\) is not pertinent. Consequently, the stability criterion presented in this article is broader and less precise.
miR-539-5p targets BMP2 to regulate Treg activation in B-cell acute lymphoblastic leukemia through TGF-β/Smads/MAPK
Authors: Dai Qingkai , Shi Rui , Zhang Ge , Wang Yuefang , Ye Lei , Peng Luyun , Guo Siqi , He Jiajing , Yang Hao , Jiang Yongmei
Published: February 2024

MicroRNAs (mRNAs) were believed to play an important role in cancers, and this study aimed to explore the mechanism of miRNA regulating Treg in B-cell acute lymphoblastic leukemia (B-ALL). Firstly, the differentially expressed miRNAs and target genes significantly associated with Tregs were screened out by high-throughput sequencing, and their enrichment pathways were analyzed. The binding relationship between miRNA and target genes was further verified, and the effects of miRNA on the proliferation and apoptosis of B-ALL Nalm-6 cells and Treg activation were analyzed. Results showed that differentially expressed miR-539-5p was significantly under-expressed, and its target gene BMP2 was significantly over-expressed in B-ALL, and significantly enriched in the TGF-β1 pathway. In addition, both miR-539-5p and BMP2 were significantly correlated with Treg activity in B-ALL. In vitro experiments further confirmed that miR-539-5p could directly target BMP2. The low expression of miR-539-5p in B-ALL significantly promoted BMP2 expression to promote the proliferation and inhibit apoptosis of Nalm-6 cells. Furthermore, the high expression of BMP2 in B-ALL could cooperate with TGF-β1 to promote the activation of human CD4+CD25-T cells to Treg, and significantly activate the TGF-β/Smads/MAPK pathway. In vivo experiments also confirmed that overexpression of miR-539-5p significantly inhibited BMP2 to suppress Treg activation and Smad1 and Smad2 phosphorylation, and finally inhibit the B-ALL process. In conclusion, miR-539-5p was significantly under-expressed in B-ALL and could target BMP2 to promote its expression, and the overexpressed BMP2 further promoted Treg activation in B-ALL by regulating TGF-β/Smads/MAPK pathway.
Pre-operative immune cell numbers and ratios are associated with peri-operative adverse outcomes in transfused patients
Authors: Roets Michelle , Sturgess David J. , Chen Ching-Yu , Punnasseril Jaisil E. J. , Jones Lee , van Zundert Andre A. , Dean Melinda M.
Published: February 2024

Background and objectives

Transfusion-related immune modulation (TRIM) and associated adverse outcomes during major surgery are increasingly important to patients and health services internationally. A panel of pre-operative blood tests is an essential part of the pre-operative anaesthetic assessment. This panel of blood tests commonly considers numbers of immune cells (i.e., lymphocytes, monocytes, and neutrophils and cell ratios) that may be used as biomarkers to evaluate and potentially predict post-operative adverse outcomes.

Design

This retrospective data collection from eight hospital databases, within the Royal Brisbane and Women's Hospital, considered only patients who received blood transfusion during surgery (2016–2018) (n = 2,121). The association between pre-operative immune cell numbers and ratios and adverse outcomes were assessed. Adverse outcomes were coded using the International Classification of Diseases-10 (ICD-10) coding which specifically considered transfusion-related immune modulation. Results were adjusted for confounding factors.

Results

After adjustment, decreased pre-operative lymphocyte numbers and increased neutrophil/lymphocyte ratio (NLR) were associated with increased odds of developing infection; decreased NLR with decreased odds of developing adverse renal outcomes; and decreased lymphocyte numbers with decreased odds of developing adverse cardiovascular outcomes. Monocyte numbers, neutrophil numbers, and the lymphocyte/monocyte ratio (LMR) were not associated with increased adverse outcomes after adjustment.

Conclusion

Pre-operative lymphocyte numbers and NLR are associated with adverse outcomes during peri-operative transfusion. Future assessment of peri-operative immune modulation should include the assessment of immune cell function and numbers.
RETRACTED: Cellular functions of spermatogonial stem cells in relation to JAK/STAT signaling pathway
Authors: Guo Xinyu , Dong Liang , Hao Dingjun
Published: February 2024

This manuscript comprehensively reviews the interrelationship between spermatogonial stem cells (SSCs) and the JAK/STAT signaling pathway. Spermatogonial stem cells in the testes of male mammals, characterized by their self-renewal and pluripotential differentiation capabilities, are essential for tissue regeneration, immunomodulation, and advancements in regenerative medicine. This review delves into the historical background and biological characteristics of SSCs, with a particular emphasis on the pivotal role of the JAK/STAT signaling pathway in their proliferation, maturation, and differentiation processes. Research indicates that the JAK/STAT pathway extensively influences various functionalities of spermatogonial stem cells, encompassing immunomodulation, tissue differentiation, homing, and adaptation to the microenvironment. Herein, we collate and dissect related studies, shedding light on the intricate dynamics between SSCs and the JAK/STAT signaling pathway, and examine the implications of these interactions on the biological attributes and functionalities of SSCs. Furthermore, the review discusses the profound implications of these findings for preclinical research and the domain of cellular engineering. It is acknowledged that, despite advancements in the research of SSCs and the JAK/STAT signaling pathway, investigations in humans and larger mammals remain inadequate, necessitating more in-depth exploration to establish a comprehensive theoretical framework. Overall, this review offers an invaluable reference for deciphering the mechanisms of the spermatogonial stem cell signaling pathways and establishes a theoretical groundwork for related preclinical research.
Efficacy and safety of tongxinluo capsule for angina pectoris of coronary heart disease: an overview of systematic reviews and meta-analysis
Authors: Li Liuying , Feng Peimin , Zhou Wenhua , Luo Biao , Deng Lvyu , Gan Daohui , Wu Xiaohan , Zhu Fengya , Zhou Xia
Published: February 2024

Background

Tongxinluo capsule (TXLC) is a common drug for treating angina pectoris of coronary heart disease (CHD). In recent years, many systematic reviews (SRs) and meta-analyses (MAs) have reported the efficacy and safety of TXLC for improving angina symptoms in patients with CHD. We aimed to comprehensively evaluate the existing SRs and MAs of TXLC in treating angina pectoris of CHD, summarize the evidence quality, and provide scientific evidence and recommendations.

Methods

We searched seven databases for relevant SRs/MAs published up to 1 June 2023. Two reviewers independently completed the literature retrieval, screening, and data extraction. We used A Measurement Tool to Assess Systematic Reviews 2 (AMSTAR 2) to evaluate the methodological quality, the Risk of Bias in Systematic Reviews (ROBIS) to assess the risk of bias, and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) to determine the strength of the evidence. RevMan 5.3 was used to synthesize data.

Results

We identified 15 SRs/MAs, including 329 RCTs and 33,417 patients. According to the evaluation results of AMSTAR-2, only one SR was of high methodological quality, the others were very low. ROBIS assessment showed that one SR (6.67%) had a low risk, 3 SRs (20%) had an unclear risk, and 11 SRs (73.33%) had a high risk. We assessed 42 outcomes by the GRADE, 10 (23.81%) for moderate-quality evidence, 17 (40.48%) for low-quality evidence, and 15 (35.71%) for very-low-quality evidence. Mate-analysis showed that TXLC combined with conventional western medications improved electrocardiogram efficacy (RR = 1.38, 95% CI: 1.23–1.43, P < 0.001) and angina efficacy (OR = 3.58, 95% CI: 3.02–4.24, P < 0.001), reduced angina attack frequency (SMD = −0.54, 95% CI: −0.64 to −0.44, P < 0.001) and angina duration (SMD = −0.42, 95% CI: −0.57 to −0.28, P < 0.001), with general heterogeneity. The pooled results showed that TXLC appears to have some efficacy in improving cardiac function and relieving angina symptoms, but there is limited evidence that it improves cardiovascular event rates, hemorheology, lipids, or hs-CRP. In the assessment of drug safety, TXLC was associated with different degrees of adverse drug reactions.

Conclusion

Based on the evidence, TXLC may be effective as an adjuvant treatment for angina pectoris of CHD. However, the quality of the evidence is low, and the drug's safety must be carefully interpreted. In future studies, high-quality randomized controlled trials are needed to confirm the effectiveness and safety of TXLC.

Systematic Review Registration

http://www.crd.york.ac.uk/PROSPERO/, identifier (CRD42022365372).
Takotsubo syndrome and atrial myxoma—identifying a new trigger: a case report
Authors: Velarde-Acosta Kevin , Sandoval Robert , Falcón-Quispe Luis , Anicama Lima William Efrain , Baltodano-Arellano Roberto
Published: February 2024

Takotsubo syndrome (TTS) is a rare cardiomyopathy, but its prevalence is increasing due to the greater availability of diagnostic tools, whose pathophysiology is unknown; however, the evidence points to an excess of catecholamines that ends up generating cardiac stunning. The cause of excessive sympathetic discharge is multifactorial, and some tumors may be related to their origin. In this case report, we present a female patient with TTS whose only identified triggering factor was an atrial myxoma, which generated an unusual clinical presentation. Current multimodal diagnostic tools together with the multidisciplinary evaluation of the HeartTeam allowed an accurate diagnosis and an adequate management of the clinical picture.
People living with HIV display increased anti-apolipoprotein A1 auto-antibodies, inflammation, and kynurenine metabolites: a case–control study
Authors: Frias Miguel A. , Pagano Sabrina , Bararpour Nasim , Sidibé Jonathan , Kamau Festus , Fétaud-Lapierre Vanessa , Hudson Peter , Thomas Aurélien , Lecour Sandrine , Strijdom Hans , Vuilleumier Nicolas
Published: February 2024

Objective

This study aimed to study the relationship between auto-antibodies against apolipoprotein A1 (anti-apoA1 IgG), human immunodeficiency virus (HIV) infection, anti-retroviral therapy (ART), and the tryptophan pathways in HIV-related cardiovascular disease.

Design

This case–control study conducted in South Africa consisted of control volunteers (n = 50), people living with HIV (PLWH) on ART (n = 50), and untreated PLWH (n = 44). Cardiovascular risk scores were determined, vascular measures were performed, and an extensive biochemical characterisation (routine, metabolomic, and inflammatory systemic profiles) was performed.

Methods

Anti-apoA1 IgG levels were assessed by an in-house ELISA. Inflammatory biomarkers were measured with the Meso Scale Discovery® platform, and kynurenine pathway metabolites were assessed using targeted metabolomic profiling conducted by liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS).

Results

Cardiovascular risk scores and vascular measures exhibited similarities across the three groups, while important differences were observed in systemic inflammatory and tryptophan pathways. Anti-apoA1 IgG seropositivity rates were 15%, 40%, and 70% in control volunteers, PLWH ART-treated, and PLWH ART-naïve, respectively. Circulating anti-apoA1 IgG levels were significantly negatively associated with CD4+ cell counts and positively associated with viremia and pro-inflammatory biomarkers (IFNγ, TNFα, MIPα, ICAM-1, VCAM-1). While circulating anti-apoA1 IgG levels were associated with increased levels of kynurenine in both control volunteers and PLWH, the kynurenine/tryptophan ratio was significantly increased in PLWH ART-treated.

Conclusion

HIV infection increases the humoral response against apoA1, which is associated with established HIV severity criteria and kynurenine pathway activation.
The paradoxes of telehealth platforms: what did we learn from the use of telehealth platforms?
Authors: Bouabida Khayreddine , Lebouché Bertrand , Pomey Marie-Pascale
Published: February 2024

This article is an overview and reflection of the findings of an evaluative study conducted on a program called “Techno-Covid Partnership” (TCP) implemented in April 2020 at the Centre Hospitalier de l’Université de Montréal (CHUM) in Montreal, Canada. In the context of the COVID-19 pandemic, the CHUM decided in April 2020 to implement telehealth, virtual care, and telemonitoring platforms and technologies to maintain access to care and reduce the risks of contamination and spread of COVID-19 as well as to protect users of health services and health professionals. Three technological platforms for telehealth and remote care and monitoring have been developed, implemented, and evaluated in real-time within the framework of the TCP program. A cross-sectional study was carried out in which a questionnaire was used and administered to users of telehealth platforms including patients and healthcare professionals. The methods and results of the study have been published previously published. In the completion of the two articles published in this context, in this paper, we briefly recall the context of the study and the method performed. The main focus of the paper is on presenting a critical overview and reflection on the major findings of our evaluation of the use of telehealth platforms from the point of view of patients and health professionals and discuss certain paradoxes i.e., the advantages, challenges, recommendations, and other perspectives that emerged in this study.
From beginning to mature: investigating the development of teacher community through Lesson Study
Authors: Ni Shuilleabhain Aoibhinn , Owens Emma , Seery Aidan , Hyland Diarmaid
Published: February 2024

This research investigates the potential of Lesson Study to develop teachers’ professional community. Situated in two case study schools, Doone and Crannog (both pseudonyms), where mathematics teachers participated in multiple, successive cycles of Lesson Study over the course of one academic year, the research details the development of teacher community through teachers’ conversations and reflections. Data was generated through audio recordings of teachers’ Lesson Study meetings and individual interviews with participating teachers. Analysis is based on a framework of professional teacher community which describes discrete phases of development from beginning to mature. Findings from the research suggest that groups of teachers may begin their Lesson Study work at different stages of evolution of teacher community and that Lesson Study has the potential to strength and develop such community over successive cycles. The research demonstrates that teachers’ participation in Lesson Study can provide them with opportunities to recognize and express communal responsibility for individual growth of colleagues, a key feature in the formation of teacher community. The research also highlights the importance of navigating fault lines or differences in ideas and opinions during the Lesson Study work. Findings may offer guidance to facilitators of Lesson Study on elements of teachers’ collaborative work which may benefit from additional focus, such as productively engaging with conflict within a group. This research contributes to the literature on Lesson Study in outlining how this model of professional development may provide a powerful structure within which teacher community can be developed.
Breaking new ground: New Zealand Certificates of Steiner Education
Authors: Boland Neil , Brice-Geard Karen , Bell Amanda , Cook Christine
Published: February 2024

The New Zealand Certificates of Steiner Education (CSE) are secondary qualifications at levels 1, 2 and 3, recognized by the New Zealand Qualifications Authority. They give access to tertiary education in New Zealand and beyond. The impulse for new qualifications grew from a wish to have important aspects of the taught Steiner curriculum recognized and valorized, that these aspects be credit bearing toward tertiary study, an option not offered by existing qualifications. The certificates were developed over an 18-month period and were implemented by the (then) four New Zealand Steiner schools with high school classes. The CSE are based on a suite of learning outcomes which give teachers a substantial degree of assessment autonomy, allowing them to tailor assessment modalities to the student or class being taught. Since 2012, the qualifications have been offered overseas and are now used as a pathway to university by Steiner schools in a growing number of countries. This article draws on the experiences of one of the developers of the qualification and two teachers in schools using the certificate in the UK, and outlines some of the challenges faced when breaking new ground in the advancement of Waldorf education internationally.
Burden of psoriasis in young adults worldwide from the global burden of disease study 2019
Authors: Zhang Yuanchen , Dong Shuai , Ma Yuan , Mou Yan
Published: February 2024

Background

To determine the global burden of psoriasis in young adults, i.e., those aged 15–49, from 1990 to 2019 and predict trends in this burden for 2020 to 2030

Methods

Age-standardized disease burden indicators and their estimated annual percentage changes were assessed and used to compare the estimated burden between regions. In addition, generalized additive models were used to predict the burden in this population from 2020 to 2030.

Results

From 1990 to 2019, the overall burden of psoriasis in young adults worldwide trended downward, as the age-standardized incidence rate and the age-standardized disability-adjusted life year rate decreased. From 1990 to 2019, there were gender differences in the burden of psoriasis between regions with different Socio-demographic index. Specifically, there was a smaller increase in the burden in young men than in young women in middle- and low–middle-Socio-demographic index areas. In 2019, Western Europe, Australasia, and Southern Latin America had the highest age-standardized incidence rate of psoriasis in young adults, whereas age-standardized disability-adjusted life year rates of psoriasis in young adults were highest in high-income North America. In 2019, the psoriasis burden in young adults was the highest in high-Socio-demographic index areas and the lowest in low-Socio-demographic index regions. We predict that from 2020 to 2030, the incidence rate and disability-adjusted life year rate of psoriasis in all age groups of young adults will continue to decline, but the burden in those aged 30–39 will increase.

Conclusion

From 1990 to 2019, the overall burden of psoriasis in each age group trended downward in this period. We predict that from 2020 to 2030, the burden of psoriasis in those aged 30–39 will increase.
Mechanism testing of the empowerment of green transformation and upgrading of industry by the digital economy in China
Authors: Liu Jiang , Zhao Qianyu
Published: February 2024

With the continuous advancement of industrialization, global environmental problems are becoming increasingly severe. Maintaining economic growth while improving the environment has been an important issue for many countries, especially developing countries. As industry is a major source of environmental pollution, industrial green transformation and upgrading have become particularly important. In the era of the digital economy (DE), there is a new path for industrial green transformation and upgrading. Based on provincial data on industry from 2008 to 2021, a difference-in-differences (DID) model was constructed to analyze the environmental and economic benefits. New pathways for trade-offs between environmental improvement and economic growth in China are presented. In addition, new ideas are concerning global environmental issues and economic issues in the DE are presented. The present study indicates that the DE has reduced the intensity of pollution emissions and elevated total factor productivity (TFP), which has helped to promote industrial green transformation and upgrading. Further mechanism testing showed that the DE has promoted industrial green transformation and upgrading by improving the utilization of energy and resources and promoting technological innovation. Considering the utilization of energy and resources, the DE has decreased China’s total industrial consumption of energy and coal, reduced industrial water consumption, and reduced the share of coal consumption and increased the share of clean energy consumption in China’s total industrial energy consumption; these effects have optimized the efficiency and structure of China’s energy utilization to contribute to the green transformation and upgrading of industry. Regarding technological innovation, the development of the DE has increased industrial innovation output and R&D input. Furthermore, it has promoted innovation with respect to green processes, accelerating technological innovation, and realized industrial green transformation and upgrading.
Drug-target identification in COVID-19 disease mechanisms using computational systems biology approaches
Authors: Niarakis Anna , Ostaszewski Marek , Mazein Alexander , Kuperstein Inna , Kutmon Martina , Gillespie Marc E. , Funahashi Akira , Acencio Marcio Luis , Hemedan Ahmed , Aichem Michael , Klein Karsten , Czauderna Tobias , Burtscher Felicia , Yamada Takahiro G. , Hiki Yusuke , Hiroi Noriko F. , Hu Finterly , Pham Nhung , Ehrhart Friederike , Willighagen Egon L. , Valdeolivas Alberto , Dugourd Aurelien , Messina Francesco , Esteban-Medina Marina , Peña-Chilet Maria , Rian Kinza , Soliman Sylvain , Aghamiri Sara Sadat , Puniya Bhanwar Lal , Naldi Aurélien , Helikar Tomáš , Singh Vidisha , Fernández Marco Fariñas , Bermudez Viviam , Tsirvouli Eirini , Montagud Arnau , Noël Vincent , Ponce-de-Leon Miguel , Maier Dieter , Bauch Angela , Gyori Benjamin M. , Bachman John A. , Luna Augustin , Piñero Janet , Furlong Laura I. , Balaur Irina , Rougny Adrien , Jarosz Yohan , Overall Rupert W. , Phair Robert , Perfetto Livia , Matthews Lisa , Rex Devasahayam Arokia Balaya , Orlic-Milacic Marija , Gomez Luis Cristobal Monraz , De Meulder Bertrand , Ravel Jean Marie , Jassal Bijay , Satagopam Venkata , Wu Guanming , Golebiewski Martin , Gawron Piotr , Calzone Laurence , Beckmann Jacques S. , Evelo Chris T. , D’Eustachio Peter , Schreiber Falk , Saez-Rodriguez Julio , Dopazo Joaquin , Kuiper Martin , Valencia Alfonso , Wolkenhauer Olaf , Kitano Hiroaki , Barillot Emmanuel , Auffray Charles , Balling Rudi , Schneider Reinhard ,   the COVID-19 Disease Map Community
Published: February 2024

Introduction

The COVID-19 Disease Map project is a large-scale community effort uniting 277 scientists from 130 Institutions around the globe. We use high-quality, mechanistic content describing SARS-CoV-2-host interactions and develop interoperable bioinformatic pipelines for novel target identification and drug repurposing.

Methods

Extensive community work allowed an impressive step forward in building interfaces between Systems Biology tools and platforms. Our framework can link biomolecules from omics data analysis and computational modelling to dysregulated pathways in a cell-, tissue- or patient-specific manner. Drug repurposing using text mining and AI-assisted analysis identified potential drugs, chemicals and microRNAs that could target the identified key factors.

Results

Results revealed drugs already tested for anti-COVID-19 efficacy, providing a mechanistic context for their mode of action, and drugs already in clinical trials for treating other diseases, never tested against COVID-19.

Discussion

The key advance is that the proposed framework is versatile and expandable, offering a significant upgrade in the arsenal for virus-host interactions and other complex pathologies.
Normalized circulating Tfh and Th17 associates with improvement in myasthenia gravis treated with ofatumumab
Authors: Song Xiaodong , He Yang , Huo Yang , Jiang Hong , Yu Yao , Sun Yue , Liu Zunjing , Zhang Zhaoxu
Published: February 2024

Objective

To assess the effect of B cell depletion therapy (BCDT) on circulating T follicular helper (cTfh) and circulating T helper 17 (cTh17) cells and its relation to clinical improvement in patients with myasthenia gravis (MG).

Methods

28 anti-AchR positive MG patients treated with ofatumumab and 28 healthy controls (HCs) were included. Frequencies of cTfh and cTh17 cells were monitored by flow cytometry at baseline and 4, and 12 weeks after the initial dose ofatumumab. Serum cytokines associated with cTfh and cTh17, including IL-6, IL-21, and IL-17, were also analyzed.

Results

The frequency of cTfh and cTh17 significantly increased in MG patients compared with HCs. Additionally, elevated levels of both T-cell subsets correlated with MG severity. During the follow-up, cTfh and cTh17 return to normal after BCDT. Furthermore, the decrease in cTfh and cTh17 was associated with MG scores improvement over time. Notably, cTfh- and cTh17-related cytokines, including IL-6, IL-21, and IL-17, exhibited a marked decrease following ofatumumab therapy.

Conclusions

Abnormal expansion of cTfh and cTh17 cells may be key features in the immunopathology of MG. Their levels returned to normal after BCDT, which was closely correlated with clinical amelioration. This result suggests that these two T-cell subsets may be targets for BCDT treatment of MG.
Influence of tidal mixing on bottom circulation in the Caroline Sea
Authors: Wang Xiaowei , Liu Chuanyu , Wang Fan
Published: February 2024

Bottom circulation in the abyssal Caroline Sea is an important component of the global meridional overturning circulation. By use of a high-resolution regional ocean model, the influence of tidal mixing processes on bottom water and circulation in the Caroline Sea is investigated. Based on different configurations for diapycnal diffusivities of tidal mixing, three numerical experiments are performed: one completely without tidal mixing, one only with local tidal mixing due to the locally dissipated tidal energy, and one considering tidal mixing processes induced by the total dissipated tidal energy. The results show that tidal mixing processes in the abyssal Caroline Sea could sustain a relatively high horizontal density gradient and hence baroclinic pressure gradient not only across the two deep-water passages connecting to the open ocean, but also within the abyssal West Caroline Basin (WCB) and East Caroline Basin (ECB). Therefore, tidal mixing processes could maintain the large amounts of bottom water inflow, intensify the bottom basin/subbasin-scale horizontal circulation, and drive a more vigorous meridional overturning circulation in the abyssal WCB and ECB. Moreover, simulations of bottom water transport in the experiment with tidal mixing processes are more consistent with previous observations and estimates. These results suggest that tidal mixing processes play a crucial dynamic role in the bottom circulation, and is essential for ocean modelling.
Effects of dietary energy level on antioxidant capability, immune function and rectal microbiota in late gestation donkeys
Authors: Guo Yongmei , Yin Guolin , Hui Fang , Guo Xiaoyu , Shi Binlin , Zhao Yanli , Yan Sumei
Published: February 2024

Introduction

This study investigated the effects of dietary energy level on the antioxidant capability, immune function, and rectal microbiota in donkey jennets during the last 60 days of gestation.

Methods

Fifteen pregnant DeZhou donkeys with age of 6.0 ± 0.1 years, body weight of 292 ± 33 kg, parity of 2.7 ± 0.1 parities and similar expected date of confinement (74 ± 4 days) were randomly allocated to three groups and feed three diets: high energy (10.92 MJ/kg, H), medium energy (10.49 MJ/kg, M), and low energy (9.94 MJ/kg, L).

Results and Discussion

The serum activity of catalase (CAT), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in group M was significantly higher, whereas the concentrations of malondialdehyde (MDA), interleukin 1 (IL-1), IL-2, and IL-6 were lower than those recorded for groups H and L (p ≤ 0.05). The dietary energy level significantly affected rectal microbial community structure in the jennet donkeys 35 days and 7 days before the parturition (p ≤ 0.05). The abundances of norank_f_norank_o_Coriobacteriales genus was significantly higher (p ≤ 0.05) in group H, and the abundances of norank_f_norank_o_Mollicutes_RF39 and the Candidatus_Saccharimonas were higher in group L (p ≤ 0.05). The abundance of Fibrobacter in group M was significantly increased (p ≤ 0.05). The abundance of norank_f_norank_o_Coriobacteriales was positively correlated with average daily gain (ADG) and tumor necrosis factor-α (TNF-α) concentrations (p ≤ 0.05). The abundance of norank_f_norank_o_Mollicutes_RF39 was positively correlated with IL-2 and IL-6 concentrations. The abundance of Candidatus_Saccharimonas was positively correlated with CAT, T-SOD and GSH-Px activities (p ≤ 0.05). The abundance of Fibrobacter was positively correlated with CAT and T-SOD activities (p ≤ 0.05), but negatively correlated with IL-2 concentration (p ≤ 0.05). In conclusion, an appropriate dietary with an energy content of 10.49 MJ/kg for jennet donkeys during late gestation increased the prenatal antioxidant capacity, reduced inflammatory cytokines, and promoted fetal growth, and these changes were related to diet-induced changes in rectal microbiota compositions.
Reforestation of Cunninghamia lanceolata changes the relative abundances of important prokaryotic families in soil
Authors: Hou Xue-Yan , Qiao Wen-Tao , Gu Ji-Dong , Liu Chao-Ying , Hussain Muhammad Mahroz , Du Dao-Lin , Zhou Yi , Wang Yong-Feng , Li Qian
Published: February 2024

Over the past decades, many forests have been converted to monoculture plantations, which might affect the soil microbial communities that are responsible for governing the soil biogeochemical processes. Understanding how reforestation efforts alter soil prokaryotic microbial communities will therefore inform forest management. In this study, the prokaryotic communities were comparatively investigated in a secondary Chinese fir forest (original) and a reforested Chinese fir plantation (reforested from a secondary Chinese fir forest) in Southern China. The results showed that reforestation changed the structure of the prokaryotic community: the relative abundances of important prokaryotic families in soil. This might be caused by the altered soil pH and organic matter content after reforestation. Soil profile layer depth was an important factor as the upper layers had a higher diversity of prokaryotes than the lower ones (p < 0.05). The composition of the prokaryotic community presented a seasonality characteristic. In addition, the results showed that the dominant phylum was Acidobacteria (58.86%) with Koribacteraceae (15.38%) as the dominant family in the secondary Chinese fir forest and the reforested plantation. Furthermore, soil organic matter, total N, hydrolyzable N, and NH4+−N were positively correlated with prokaryotic diversity (p < 0.05). Also, organic matter and NO3-−N were positively correlated to prokaryotic abundance (p < 0.05). This study demonstrated that re-forest transformation altered soil properties, which lead to the changes in microbial composition. The changes in microbial community might in turn influence biogeochemical processes and the environmental variables. The study could contribute to forest management and policy-making.
Multi-omics revealed rumen microbiota metabolism and host immune regulation in Tibetan sheep of different ages
Authors: Sha Yuzhu , Liu Xiu , He Yanyu , Zhao Shengguo , Hu Jiang , Wang Jiqing , Li Wenhao , Shao Pengyang , Wang Fanxiong , Chen Xiaowei , Yang Wenxin , Xie Zhuanhui
Published: February 2024

The rumen microbiota and metabolites play an important role in energy metabolism and immune regulation of the host. However, the regulatory mechanism of rumen microbiota and metabolite interactions with host on Tibetan sheep’s plateau adaptability is still unclear. We analyzed the ruminal microbiome and metabolome, host transcriptome and serum metabolome characteristics of Tibetan sheep at different ages. Biomarkers Butyrivibrio, Lachnospiraceae_XPB1014_group, Prevotella, and Rikenellaceae_RC9_gut_group were found in 4 months, 1.5 years, 3.5 years, and 6 years Tibetan sheep, respectively. The rumen microbial metabolites were mainly enriched in galactose metabolism, unsaturated fatty acid biosynthesis and fatty acid degradation pathways, and had significant correlation with microbiota. These metabolites further interact with mRNA, and are co-enriched in arginine and proline metabolism, metabolism of xenobiotics by cytochrome P450, propanoate metabolism, starch and sucrose metabolism, gap junction pathway. Meanwhile, serum metabolites also have a similar function, such as chemical carcinogenesis − reactive oxygen species, limonene and pinene degradation, and cutin, suberine and wax biosynthesis, thus participating in the regulation of the body’s immune and energy-related metabolic processes. This study systematically revealed that rumen microbiota, metabolites, mRNA and serum metabolites of Tibetan sheep were involved in the regulation of fermentation metabolic function and immune level of Tibetan sheep at different ages, which provided a new perspective for plateau adaptability research of Tibetan sheep at different ages.
Apoptosis signal-regulating kinase 1 (Ask1) deficiency alleviates MPP+-induced impairment of evoked dopamine release in the mouse hippocampus
Authors: Zhao Fang , Li Chuhan , Zhuang Yinghan , Yan Yan , Gao Yanqin , Behnisch Thomas
Published: February 2024

The dopaminergic system is susceptible to dysfunction in numerous neurological diseases, including Parkinson’s disease (PD). In addition to motor symptoms, some PD patients may experience non-motor symptoms, including cognitive and memory deficits. A possible explanation for their manifestation is a disturbed pattern of dopamine release in brain regions involved in learning and memory, such as the hippocampus. Therefore, investigating neuropathological alterations in dopamine release prior to neurodegeneration is imperative. This study aimed to characterize evoked hippocampal dopamine release and assess the impact of the neurotoxin MPP+ using a genetically encoded dopamine sensor and gene expression analysis. Additionally, considering the potential neuroprotective attributes demonstrated by apoptosis signal-regulating kinase 1 (Ask1) in various animal-disease-like models, the study also aimed to determine whether Ask1 knockdown restores MPP+-altered dopamine release in acute hippocampal slices. We applied variations of low- and high-frequency stimulation to evoke dopamine release within different hippocampal regions and discovered that acute application of MPP+ reduced the amount of dopamine released and hindered the recovery of dopamine release after repeated stimulation. In addition, we observed that Ask1 deficiency attenuated the detrimental effects of MPP+ on the recovery of dopamine release after repeated stimulation. RNA sequencing analysis indicated that genes associated with the synaptic pathways are involved in response to MPP+ exposure. Notably, Ask1 deficiency was found to downregulate the expression of Slc5a7, a gene encoding a sodium-dependent high-affinity choline transporter that regulates acetylcholine levels. Respective follow-up experiments indicated that Slc5a7 plays a role in Ask1 deficiency-mediated protection against MPP+ neurotoxicity. In addition, increasing acetylcholine levels using an acetylcholinesterase inhibitor could exacerbate the toxicity of MPP+. In conclusion, our data imply that the modulation of the dopamine-acetylcholine balance may be a crucial mechanism of action underlying the neuroprotective effects of Ask1 deficiency in PD.
Quantitative evaluation of swallowing function in Parkinson’s disease using tongue pressure measurement: a mini-review
Authors: Fukuoka Tatsuyuki , Hori Kazuhiro , Ono Takahiro
Published: February 2024

Dysphagia is a common symptom of Parkinson’s disease (PD) associated with aspiration pneumonia, choking, malnutrition, and a decreased quality of life, and is a leading cause of death among patients with PD. Tongue dysfunction in patients with PD affects the oral phase of swallowing, including the formation and propulsion of a bolus into the pharynx. Assessing tongue pressure, generated between the tongue and palate, is a method that quantitatively measures tongue function and is related to dysphagia in PD. Two assessment methods are used to measure tongue pressure: tongue strength and tongue pressure during swallowing. Previous studies measuring tongue pressure in PD have reported decreased tongue strength and pressure during swallowing, as well as a prolonged tongue pressure rise time, which are symptoms associated with PD severity and dysphagia. In this mini-review, we present a method for measuring tongue pressure and discuss its relationship with dysphagia in PD. We also describe limitations and future perspectives in tongue pressure measurement research.
Disrupted properties of functional brain networks in major depressive disorder during emotional face recognition: an EEG study via graph theory analysis
Authors: Teng Chao-Lin , Cong Lin , Wang Wei , Cheng Shan , Wu Min , Dang Wei-Tao , Jia Min , Ma Jin , Xu Jin , Hu Wen-Dong
Published: February 2024

Previous neuroimaging studies have revealed abnormal brain networks in patients with major depressive disorder (MDD) in emotional processing. While any cognitive task consists of a series of stages, little is yet known about the topology of functional brain networks in MDD for these stages during emotional face recognition. To address this problem, electroencephalography (EEG)-based functional brain networks of MDD patients at different stages of facial information processing were investigated in this study. First, EEG signals were collected from 16 patients with MDD and 18 age-, gender-, and education-matched normal subjects when performing an emotional face recognition task. Second, the global field power (GFP) method was employed to divide group-averaged event-related potentials into different stages. Third, using the phase transfer entropy (PTE) approach, the brain networks of MDD patients and normal individuals were constructed for each stage in negative and positive face processing, respectively. Finally, we compared the topological properties of brain networks of each stage between the two groups using graph theory approaches. The results showed that the analyzed three stages of emotional face processing corresponded to specific neurophysiological phases, namely, visual perception, face recognition, and emotional decision-making. It was also demonstrated that depressed patients showed abnormally decreased characteristic path length at the visual perception stage of negative face recognition and normalized characteristic path length in the stage of emotional decision-making during positive face processing compared to healthy subjects. Furthermore, while both the MDD and normal groups’ brain networks were found to exhibit small-world network characteristics, the brain network of patients with depression tended to be randomized. Moreover, for patients with MDD, the centro-parietal region may lose its status as a hub in the process of facial expression identification. Together, our findings suggested that altered emotional function in MDD patients might be associated with disruptions in the topological organization of functional brain networks during emotional face recognition, which further deepened our understanding of the emotion processing dysfunction underlying MDD.
Preparation of sea buckthorn (Hippophae rhamnoides L.) seed meal peptide by mixed fermentation and its effect on volatile compounds and hypoglycemia
Authors: Yang Jiangyong , Hong Jingyang , Aihaiti Aihemaitijiang , Mu Ying , Yin Xuefeng , Zhang Minwei , Liu Xiaolu , Wang Liang
Published: February 2024

This study employed mixed bacterial strains to ferment seabuckthorn seed meal into peptides, and conducted a comprehensive evaluation of the growth adaptive conditions, molecular weight distribution, volatile compounds, and in vitro hypoglycemic activity required for fermentation. Results showed that when the amount of maltose was 1.1% and MgSO4·7H2O was added at 0.15 g/L, the peptide yield reached 43.85% with a mixed fermentation of Lactobacillus fermentum, Bacillus subtilis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Components with a molecular weight below 1 kDa were found to be more effective in inhibiting the activity of α-amylase and α-glucosidase, with the identified sequence being FYLPKM. Finally, SPME/GC–MS results showed that 86 volatile components were detected during the fermentation of seabuckthorn seed meal, including 22 alcohols, 9 acids, 7 ketones, 14 alkanes, 20 esters, and 14 other compounds. With prolonged fermentation time, the content of acids and esters increased significantly.
Pairing taVNS and CIMT is feasible and may improve upper extremity function in infants
Authors: McGloon Kelly , Humanitzki Elizabeth , Brennan Julia , Summers Philip , Brennan Alyssa , George Mark S. , Badran Bashar W. , Cribb Anne R. , Jenkins Dorothea , Coker-Bolt Patricia
Published: February 2024

In this study we combined non-invasive transcutaneous auricular vagal nerve stimulation (taVNS) with 40 h of constraint induced movement therapy (CIMT) in infants. All infants completed the full intervention with no adverse events. Therapists were able to maintain high treatment fidelity and reported high ratings for ease of use and child tolerance. Preliminary results show promising gains on motor outcomes: Mean QUEST increase 19.17 (minimal clinically important difference, MCID 4.89); Mean GMFM increase 13.33 (MCID 1%–3%). Infants also exceeded expectations on Goal Attainment Scores (+1). Early data is promising that taVNS paired with intensive motor CIMT is feasible, reliable, and safe in young infants with hemiplegia, and may help harness activity-dependent plasticity to enhance functional movement.
Long-term space missions’ effects on the human organism: what we do know and what requires further research
Authors: Tomsia Marcin , Cieśla Julia , Śmieszek Joanna , Florek Szymon , Macionga Agata , Michalczyk Katarzyna , Stygar Dominika
Published: February 2024

Space has always fascinated people. Many years have passed since the first spaceflight, and in addition to the enormous technological progress, the level of understanding of human physiology in space is also increasing. The presented paper aims to summarize the recent research findings on the influence of the space environment (microgravity, pressure differences, cosmic radiation, etc.) on the human body systems during short-term and long-term space missions. The review also presents the biggest challenges and problems that must be solved in order to extend safely the time of human stay in space. In the era of increasing engineering capabilities, plans to colonize other planets, and the growing interest in commercial space flights, the most topical issues of modern medicine seems to be understanding the effects of long-term stay in space, and finding solutions to minimize the harmful effects of the space environment on the human body.
Experimental investigation of freeze injury temperatures in trees and their contributing factors based on electrical impedance spectroscopy
Authors: Song Xinyu , Gao Tong , Ai Mengyao , Gao Shan
Published: February 2024

In trees, injuries resulting from subfreezing temperatures can cause damage to the cellular biofilm system, metabolic functions, and fibrous reticulum, and even cell death. Investigating the occurrence of freezing damage and its contributing factors could help understand the mechanisms underlying freezing injury and prevent the subsequent damage in trees. To achieve this, a laboratory experiment was conducted using cut wood samples from Korean pine (Pinus koraiensis Siebold & Zucc) and Simon poplar (Populus simonii Carr.), and the effects of environmental freezing factors, including freezing temperatures, freezing duration, and cooling rate, on the temperature at which freezing injuries occur were examined using the electrical impedance spectroscopy (EIS) method. The semi-lethal temperature (LT50), as an indicator of freezing injury in wood tissue, was theoretically deduced based on the measured extracellular resistance (re) using EIS. The contributory factors to changes in LT50 were determined and their relationship was established. The results revealed that all freezing factors exhibited significant effects on electrical impedance characteristics (re, ri, and τ), significantly influencing the LT50 of the wood. Random forest (RF) and support vector machine (SVM) models were used to assess the contribution of the freezing factors and moisture content (MC). Among the factors examined, freezing duration had the greatest impact on LT50, followed by the MC, whereas the contribution of the cooling rate was minimal. The model accuracies were 0.89 and 0.86 for Korean pine and Simon poplar, respectively. The findings of our study illustrate that the occurrence of freezing injury in trees is primarily influenced by the duration of freezing at specific subzero temperatures. Slow cooling combined with prolonged freezing at low subzero temperatures leads to earlier and more severe freezing damage.
The influence of perceived teacher support on online English learning engagement among Chinese university students: a cross-sectional study on the mediating effects of self-regulation
Authors: Yin Danting , Luo Lin
Published: February 2024

Objectives

This study examines the association between perceived teacher support and self-regulation in learning, and their combined relationship with online English learning engagement among university students in China. The objective is to uncover the underlying mechanisms of this relationship, with a particular focus on the role of self-regulation in learning as a mediator.

Methods

The study involved 1,361 university students from Southwest China, predominantly female (73.84%) with an average age of 18.94 years (SD = 1.07). Refined measurement tools were employed to assess perceived teacher support, online English learning engagement, and self-regulation in learning.

Results

The findings indicate that components of self-regulation, such as goal setting, environmental structuring, and time management, act as full mediators in the relationship between perceived teacher support and online English learning engagement.

Conclusion

This research underscores the importance of self-regulation in learning in linking perceived teacher support with online English learning engagement. The insights gained are crucial for enhancing teaching strategies in online English language education.