bluuebunny/crossref_metadata_2025 huggingface dataset:
Friction and Wear of Pd-Rich Amorphous Alloy (Pd43Cu27Ni10P20) with Ionic Liquid (IL) as Lubricant at High Temperatures
Authors: Lee Jaeho , Yeo Chang-Dong , Hu Zhonglue , Thalangama-Arachchige Vidura D. , Kaur Jagdeep , Quitevis Edward L. , Kumar Golden , Koh Yung P. , Simon Sindee
Published: November 2019

The friction and wear behavior of palladium (Pd)-rich amorphous alloy (Pd43Cu27Ni10P20) against 440C stainless steel under ionic liquids as lubricants, i.e., 1-nonyl-3-methylimidazolium bis[(trifluoromethane)sulfonyl]amide ([C9C1im][NTf2]), were investigated using a ball-on-disc reciprocating tribometer at ambient, 100 and 200 °C with different sliding speeds of 3 and 7 mm/s, whose results were compared to those from crystalline Pd samples. The measured coefficient of friction (COF) and wear were affected by both temperature and sliding speed. The COF of crystalline Pd samples dramatically increased when the temperature increased, whereas the COF of the amorphous Pd alloy samples remained low. As the sliding speed increased, the COF of both Pd samples showed decreasing trends. From the analysis of a 3D surface profilometer and scanning electron microscopy (SEM) with electron dispersive spectroscopy (EDS) data, three types of wear (i.e., delamination, adhesive, and abrasive wear) were observed on the crystalline Pd surfaces, whereas the amorphous Pd alloy surfaces produced abrasive wear only. In addition, X-ray photoelectron spectroscopy (XPS) measurements were performed to study the formation of tribofilm. It was found that the chemical reactivity at the contacting interface increased with temperature and sliding contact speed. The ionic liquids (ILs) were effective as lubricants when the applied temperature and sliding speed were 200 °C and 7 mm/s, respectively.
Texture Evolution in Biocompatible Mg-Y-Re Alloy After Friction Stir Processing
Authors: Kunčická Lenka , Král Petr , Dvořák Jiří , Kocich Radim
Published: November 2019

The presented study deals with the investigation of biocompatible WE 43 Mg-based alloy processed via the combination of rotary swaging (RS) and friction stir processing (FSP) at three different rotational speeds of 400 RPM, 800 RPM, and 1200 RPM. The structure observations primarily focused on texture development and characterizations of grain sizes and grain boundaries. The results showed that swaging plus processing at 400 RPM and 1200 RPM lead to substantial recrystallization and grain refinement. The fractions of low angle grain boundaries within the 400 RPM and 1200 RPM samples were approximately 11%, while for the 800 RPM sample exhibiting secondary recrystallization it was about 22%. The grains were also the finest in the 1200 RPM sample (average grain diameter of 1.8 µm). The processed structures exhibited a slight tendency to form the {10-10} <0001> preferential fiber texture (especially the 800 RPM sample). Tensile testing showed the FSP to have positive influence on the ultimate tensile stress, as well as ductility of all the samples; the mechanical properties improved with increasing FSP rate.
Lipid Profile Characterization and Lipoprotein Comparison of Extracellular Vesicles from Human Plasma and Serum
Authors: Sun Yuchen , Saito Kosuke , Saito Yoshiro
Published: November 2019

Extracellular vesicles (EVs) consist of lipid bilayers, occur in various biofluids, and are invaluable in biomarker screening. Liquid chromatography coupled with high-resolution mass spectrometry (LC-MS) was recently used to study comprehensive EV lipid profiles in vitro. The aim of this study was to establish a lipidomics platform for human plasma and serum EVs for comprehensive characterization of their lipid profiles, and to compare them with those of other lipid-containing particles, such as high-density lipoproteins (HDL), and low/very low-density lipoproteins (LDL/VLDL). Isolation was validated by specific protein markers; CD9 and MHC class I for EVs, apoA-I for HDL, and apoB-100 for LDL/VLDL. Lipidomics identified 264 lipids from isolated plasma EVs, HDL, and LDL/VLDL. The absolute lipid levels per unit protein content in the EVs were more than eight times lower than those of the lipoproteins. Moreover, the EVs had higher lysoglycerophospholipid levels than HDL or LDL/VLDL. Similar profiles were also determined for human serum. The present study found that the lipid profiles of EVs are unique and distinctly different from those of lipoproteins. The lipidomics platform applied to human plasma and serum EVs could generate important information for the exploration and qualification of biomarkers in disease diagnosis.
The Indentation-Induced Pop-in Phenomenon and Fracture Behaviors of GaP(100) Single-Crystal
Authors: Chiu Yi-Jui , Jian Sheng-Rui , Lee Jyh-Wei , Juang Jenh-Yih
Published: November 2019

The deformation behaviors and fracture features of GaP(100) single-crystal are investigated by using nano- and micro-scale indentation techniques. The hardness and Young’s modulus were measured by nanoindentation using a Berkovich diamond indenter with continuous contact stiffness measurements (CSM) mode and the values obtained were 12.5 ± 1.2 GPa and 152.6 ± 12.8 GPa, respectively. In addition, the characteristic “pop-in” was observed in the loading portion of load-displacement curve, which was caused by the nucleation and/or propagation of dislocations. An energetic estimation methodology on the associated nanoindentation-induced dislocation numbers resulting from the pop-in events was discussed. Furthermore, the Vickers indentation induced fracture patterns of GaP(100) single-crystal were observed and analyzed using optical microscopy. The obtained fracture toughness KC of GaP(100) single-crystal was ~1.7 ± 0.1 MPa·m1/2, which is substantially higher than the KIC values of 0.8 MPa·m1/2 and 1.0 MPa·m1/2 previously reported for of single-crystal and polycrystalline GaP, respectively.
Investigation on Ambipolar Current Suppression Using a Stacked Gate in an L-shaped Tunnel Field-Effect Transistor
Authors: Yu Junsu , Kim Sihyun , Ryu Donghyun , Lee Kitae , Kim Changha , Lee Jong-Ho , Kim Sangwan , Park Byung-Gook
Published: November 2019

L-shaped tunnel field-effect transistor (TFET) provides higher on-current than a conventional TFET through band-to-band tunneling in the vertical direction of the channel. However, L-shaped TFET is disadvantageous for low-power applications because of increased off-current due to the large ambipolar current. In this paper, a stacked gate L-shaped TFET is proposed for suppression of ambipolar current. Stacked gates can be easily implemented using the structural features of L-shaped TFET, and on- and off-current can be controlled separately by using different gates located near the source and the drain, respectively. As a result, the suppression of ambipolarity is observed with respect to work function difference between two gates by simulation of the band-to-band tunneling generation. Furthermore, the proposed device suppresses ambipolar current better than existing ambipolar current suppression methods. In particular, low drain resistance is achieved as there is no need to reduce drain doping, which leads to a 7% enhanced on-current. Finally, we present the fabrication method for a stacked gate L-shaped TFET.
Allium sativum Extract Chemical Composition, Antioxidant Activity and Antifungal Effect against Meyerozyma guilliermondii and Rhodotorula mucilaginosa Causing Onychomycosis
Authors: Pârvu Marcel , Moţ Cătălin A. , Pârvu Alina E. , Mircea Cristina , Stoeber Leander , Roşca-Casian Oana , Ţigu Adrian B.
Published: November 2019

Onychomycosis is a major health problem due to its chronicity and resistance to therapy. Because some cases associate paronychia, any therapy must target the fungus and the inflammation. Medicinal plants represent an alternative for onychomycosis control. In the present work the antifungal and antioxidant activities of Alium sativum extract against Meyerozyma guilliermondii (Wick.) Kurtzman & M. Suzuki and Rhodotorula mucilaginosa (A. Jörg.) F.C. Harrison, isolated for the first time from a toenail onychomycosis case, were investigated. The fungal species were confirmed by DNA molecular analysis. A. sativum minimum inhibitory concentration (MIC) and ultrastructural effects were examined. At the MIC concentration (120 mg/mL) the micrographs indicated severe structural alterations with cell death. The antioxidant properties of the A. sativum extract were evaluated is a rat turpentine oil induced inflammation, and compared to an anti-inflammatory drug, diclofenac, and the main compound from the extract, allicin. A. sativum reduced serum total oxidative status, malondialdehyde and nitric oxide production, and increased total thiols. The effects were comparable to those of allicin and diclofenac. In conclusion, the garlic extract had antifungal effects against M. guilliermondii and R. mucilaginosa, and antioxidant effect in turpentine-induced inflammation. Together, the antifungal and antioxidant activities support that A. sativum is a potential alternative treatment in onychomycosis.
Function of Tetra (4-Aminophenyl) Porphyrin in Altering the Electronic Performances of Reduced Graphene Oxide-Based Field Effect Transistor
Authors: Hu Shihui , Jia Yunfang
Published: November 2019

Porphyrin functionalized reduced graphene oxide (rGO) is attractive for multi-disciplinary research studies, and its improvements for an rGO-based field effect transistor (rGO-FET) were exploited to realize ultrasensitive biochemical and clinical assay. Although it was believed that the hybrids of porphyrin and rGO can make positive impacts on the rGO-FET’s electronic performances, the understandings of its functions are still piecemeal. Herein, the reduced mixtures of tetra (4-aminophenyl) porphyrin (TAP), GO (TAP-rGO), and the FET channeled by them are examined to throw a light on the possible approaches through which TAP affects rGO’s quality and its carrier mobilities. A TAP-caused game relationship is established by deliberating about the results of the intentionally altered experimental conditions, including TAP contents and the overmixing pretreatment. The p-type doping deduction for the right-shifted ambipolar transfer characteristic curves is evidenced by X-ray photoelectron spectroscopy (XPS). The problems posed by the TAP-induced FET features’ improvement, regression, and deterioration are clarified by the integrated proofs from Raman fingerprints, the amide and carboxyl groups’ changing trajectory found by C1s XPS core spectra, and the enlarged few-layer graphene morphology from atomic force microscope and transmission electron microscope. We hope that this effort will provide some constructive recommendations for producing low-cost graphene derivatives and promoting their applications in FET-like electronic components.
Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action
Authors: Bergman Matthew E. , Davis Benjamin , Phillips Michael A.
Published: November 2019

Specialized plant terpenoids have found fortuitous uses in medicine due to their evolutionary and biochemical selection for biological activity in animals. However, these highly functionalized natural products are produced through complex biosynthetic pathways for which we have a complete understanding in only a few cases. Here we review some of the most effective and promising plant terpenoids that are currently used in medicine and medical research and provide updates on their biosynthesis, natural occurrence, and mechanism of action in the body. This includes pharmacologically useful plastidic terpenoids such as p-menthane monoterpenoids, cannabinoids, paclitaxel (taxol®), and ingenol mebutate which are derived from the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, as well as cytosolic terpenoids such as thapsigargin and artemisinin produced through the mevalonate (MVA) pathway. We further provide a review of the MEP and MVA precursor pathways which supply the carbon skeletons for the downstream transformations yielding these medically significant natural products.
Flame Retardant Epoxy Composites on the Road of Innovation: An Analysis with Flame Retardancy Index for Future Development
Authors: Movahedifar Elnaz , Vahabi Henri , Saeb Mohammad Reza , Thomas Sabu
Published: November 2019

Nowadays, epoxy composites are elements of engineering materials and systems. Although they are known as versatile materials, epoxy resins suffer from high flammability. In this sense, flame retardancy analysis has been recognized as an undeniable requirement for developing future generations of epoxy-based systems. A considerable proportion of the literature on epoxy composites has been devoted to the use of phosphorus-based additives. Nevertheless, innovative flame retardants have coincidentally been under investigation to meet market requirements. This review paper attempts to give an overview of the research on flame retardant epoxy composites by classification of literature in terms of phosphorus (P), non-phosphorus (NP), and combinations of P/NP additives. A comprehensive set of data on cone calorimetry measurements applied on P-, NP-, and P/NP-incorporated epoxy systems was collected and treated. The performance of epoxy composites was qualitatively discussed as Poor, Good, and Excellent cases identified and distinguished by the use of the universal Flame Retardancy Index (FRI). Moreover, evaluations were rechecked by considering the UL-94 test data in four groups as V0, V1, V2, and nonrated (NR). The dimensionless FRI allowed for comparison between flame retardancy performances of epoxy composites. The results of this survey can pave the way for future innovations in developing flame-retardant additives for epoxy.
ZnO@TiO2 Core Shell Nanorod Arrays with Tailored Structural, Electrical, and Optical Properties for Photovoltaic Application
Authors: Panžić Ivana , Juraić Krunoslav , Krstulović Nikša , Šantić Ana , Belić Domagoj , Blažeka Damjan , Plodinec Milivoj , Mandić Vilko , Macan Jelena , Hammud Adnan , Ivanov Danail , Plaisier Jasper , Willinger Marc Gregor , Gracin Davor , Gajović Andreja
Published: November 2019

ZnO has prominent electron transport and optical properties, beneficial for photovoltaic application, but its surface is prone to the formation of defects. To overcome this problem, we deposited nanostructured TiO2 thin film on ZnO nanorods to form a stable shell. ZnO nanorods synthesized by wet-chemistry are single crystals. Three different procedures for deposition of TiO2 were applied. The influence of preparation methods and parameters on the structure, morphology, electrical and optical properties were studied. Nanostructured TiO2 shells show different morphologies dependent on deposition methods: (1) separated nanoparticles (by pulsed laser deposition (PLD) in Ar), (2) a layer with nonhomogeneous thickness (by PLD in vacuum or DC reactive magnetron sputtering), and (3) a homogenous thin layer along the nanorods (by chemical deposition). Based on the structural study, we chose the preparation parameters to obtain an anatase structure of the TiO2 shell. Impedance spectroscopy shows pure electron conductivity that was considerably better in all the ZnO@TiO2 than in bare ZnO nanorods or TiO2 layers. The best conductivity among the studied samples and the lowest activation energy was observed for the sample with a chemically deposited TiO2 shell. Higher transparency in the visible part of spectrum was achieved for the sample with a homogenous TiO2 layer along the nanorods, then in the samples with a layer of varying thickness.
New Chemical Constituents from the Bark of Dendropanax morbifera Leveille and Their Evaluation of Antioxidant Activities
Authors: Chung Ill-Min , Kim Seung-Hyun , Kwon Chang , Kim So-Yeon , Yang Yu-Jin , Kim Ju-Sung , Ali Mohd , Ahmad Ateeque
Published: November 2019

Four new constituents, as cis-6-oxogeran-4-enyl-10-oxy-O-β-arabinopyranosyl-4′-O-β-arabinopyranosyl-2″-octadec-9‴,12‴,15‴-trienoate (1), geran-3(10)-enyl-1-oxy-O-β-arabinopyranosyl-4′-O-β-arabinopyranosyl-2″-octadec-9‴,12‴,15‴-trienoate (2), geranilan-8-oxy-O-α-d-xylopyranosyl-2′-n-octadec-9″,12″,15″-trienoate (3), 1-cyclohex-2′, 5′-dienyl 1-cyclohexylethanol-O-β-d-xylopyranoside (4), along with six known constituents, guaiacol-O-β-d-arabinopyaranoside (5), n-tetradecanyl oleate (6), oleyl-O-β-d-xyloside (7), n-octadec-9,12-dienoyl-O-β-d-arabinopyranoside (8), linolenyl-O-β-d-arabinofuranoside (9) andglyceryl-1,3-dipalmito-2-olein (10), were isolated and identified from the Dendropanax morbifera bark. The new structures were established by one-and two-dimensional NMR (and in combination with IR, FAB-MSand HR-ESI-FTMS. The comparative evaluation of antioxidant potential by phosphomolybdenum, DPPH, FRAP and the NO assay of four different compounds (1–4), we have found that the compounds 1 and 2 have power as a natural antioxidant, whereas the compound 3 and 4 exhibited mild activity in comparison to compounds 1 and 2.
Alkaline Extraction for Lead Determination in Different Types of Commercial Paints
Authors: Romero-Estévez David , Yánez-Jácome Gabriela S. , Simbaña-Farinango Karina , Vélez-Terreros Pamela Y , Navarrete Hugo
Published: November 2019

In 2017, the World Health Organization and the United Nations Environment Programme formed the Global Alliance to Eliminate Lead Paint. All alliance member countries have pledged to develop control regulations that include lead threshold limits. To improve regulations and demonstrate compliance of paint industry products, it is necessary to have adequate, locally applicable methodologies. In this sense, the main objective of this research was to validate the methodology of alkaline extraction for the quantification of lead in ten different types of Ecuadorian commercial paints using flame atomic absorption spectrophotometry. Two hundred and fifty samples from different paint industry products were analyzed, and the results were used to evaluate the method’s performance and robustness. It was determined that the method could be applied for lead concentrations above 100 mg·kg−1, and results showed relative standard deviation values lower than 14.8% and fortification recoveries between 80.3 and 119.4%, fulfilling the acceptance criteria established in the Environmental Protection Agency’s lead-based Paint Laboratory Operations Guidelines.
Design and Simple Assembly of Gold Nanostar Bioconjugates for Surface-Enhanced Raman Spectroscopy Immunoassays
Authors: Oliveira Maria João , P. de Almeida Miguel , Nunes Daniela , Fortunato Elvira , Martins Rodrigo , Pereira Eulália , J. Byrne Hugh , Águas Hugo , Franco Ricardo
Published: November 2019

Immunoassays using Surface-Enhanced Raman Spectroscopy are especially interesting on account not only of their increased sensitivity, but also due to its easy translation to point-of-care formats. The bases for these assays are bioconjugates of polyclonal antibodies and anisotropic gold nanoparticles functionalized with a Raman reporter. These bioconjugates, once loaded with the antigen analyte, can react on a sandwich format with the same antibodies immobilized on a surface. This surface can then be used for detection, on a microfluidics or immunochromatographic platform. Here, we have assembled bioconjugates of gold nanostars functionalized with 4-mercaptobenzoic acid, and anti-horseradish peroxidase antibodies. The assembly was by simple incubation, and agarose gel electrophoresis determined a high gold nanostar to antibody binding constant. The functionality of the bioconjugates is easy to determine since the respective antigen presents peroxidase enzymatic activity. Furthermore, the chosen antibody is a generic immunoglobulin G (IgG) antibody, opening the application of these principles to other antibody-antigen systems. Surface-Enhanced Raman Spectroscopy analysis of these bioconjugates indicated antigen detection down to 50 µU of peroxidase activity. All steps of conjugation were fully characterized by ultraviolet-visible spectroscopy, dynamic light scattering, ζ -Potential, scanning electron microscopy, and agarose gel electrophoresis. Based on the latter technique, a proof-of-concept was established for the proposed immunoassay.
Prospective Analysis of Food Consumption and Nutritional Status and the Impact on the Dietary Inflammatory Index in Women with Breast Cancer during Chemotherapy
Authors: Custódio Isis Danyelle Dias , Franco Fernanda de Paula , Marinho Eduarda da Costa , Pereira Taísa Sabrina Silva , Lima Mariana Tavares Miranda , Molina Maria del Carmen Bisi , Shivappa Nitin , Hebert James R. , Paiva Carlos Eduardo , Maia Yara Cristina de Paiva
Published: November 2019

Considering the implications of adverse effects of chemotherapy (CT) and the potential impact of diet on patients’ recovery, this study aimed to prospectively evaluate the association between the consumption of food groups, patients’ Dietary Inflammatory Index (DII®) scores, and their nutritional status. Anthropometric and dietary assessments of 55 women with breast cancer (BC) were performed at three time points. T0 is the time point after the first CT cycle, T1 is the time point after the intermediate CT cycle, and T2 is the time point after the last CT cycle. We identified a significant increase in weight, body mass index, and waist circumference during CT. Consumption of poultry and eggs was higher in T1 when compared to T2, while consumption of total fruit and total vegetables was higher at T0 compared to T1 and T2. The diet became more pro-inflammatory over the course of treatment (X2(2) = 61.127), and was related to higher abdominal adiposity. Total fruit (T0: R2 = 0.208, T1: R2 = 0.095, T2: R2 = 0.120) and total vegetable consumption (T0: R2 = 0.284, T1: R2 = 0.365, T2: R2 = 0.580) predicted DII® change at the three-time points. Meanwhile, consumption of total grains was significantly associated only with T1 (R2 = 0.084) and T2 (R2 = 0.118), and consumption of simple sugars was significantly associated only with T0 (R2 = 0.137) and T1 (R2 = 0.126). Changes in food consumption led to an increase in the inflammatory profile of the diet, suggesting the necessity to improve the guidelines during and after CT. These results reinforce the need to promote healthier eating practices in concert with maintaining a healthy nutritional status in women with BC treated with CT.
ER-Negative Breast Cancer Is Highly Responsive to Cholesterol Metabolite Signalling
Authors: Hutchinson Samantha A , Lianto Priscilia , Roberg-Larsen Hanne , Battaglia Sebastiano , Hughes Thomas A , Thorne James L
Published: November 2019

Interventions that alter cholesterol have differential impacts on hormone receptor positive- and negative-breast cancer risk and prognosis. This implies differential regulation or response to cholesterol within different breast cancer subtypes. We evaluated differences in side-chain hydroxycholesterol and liver X nuclear receptor signalling between Oestrogen Receptor (ER)-positive and ER-negative breast cancers and cell lines. Cell line models of ER-positive and ER-negative disease were treated with Liver X Receptor (LXR) ligands and transcriptional activity assessed using luciferase reporters, qPCR and MTT. Publicly available datasets were mined to identify differences between ER-negative and ER-positive tumours and siRNA was used to suppress candidate regulators. Compared to ER-positive breast cancer, ER-negative breast cancer cells were highly responsive to LXR agonists. In primary disease and cell lines LXRA expression was strongly correlated with its target genes in ER-negative but not ER-positive disease. Expression of LXR’s corepressors (NCOR1, NCOR2 and LCOR) was significantly higher in ER-positive disease relative to ER-negative, and their knock-down equalized sensitivity to ligand between subtypes in reporter, gene expression and viability assays. Our data support further evaluation of dietary and pharmacological targeting of cholesterol metabolism as an adjunct to existing therapies for ER-negative and ER-positive breast cancer patients.
Factors Influencing British Adolescents’ Intake of Whole Grains: A Pilot Feasibility Study Using SenseCam Assisted Interviews
Authors: Kamar Maya , Evans Charlotte , Hugh-Jones Siobhan
Published: November 2019

High whole grain intake is beneficial for health. However, adolescents consume low levels of whole grain and the understanding of the underpinning reasons for this is poor. Using a visual, participatory method, we carried out a pilot feasibility study to elicit in-depth accounts of young people’s whole grain consumption that were sensitive to their dietary, familial and social context. Furthermore, we explored barriers and suggested facilitators to whole grain intake and assessed the feasibility of using SenseCam to engage adolescents in research. Eight British adolescents (aged 11 to 16 years) wore a SenseCam device which auto-captured images every twenty seconds for three consecutive days. Participants then completed traditional 24-hour dietary recalls followed by in-depth interviews based on day three SenseCam images. Interview data were subjected to thematic analysis. Findings revealed that low adolescent whole grain intake was often due to difficulty in identifying whole grain products and their health benefits; and because of poor availability in and outside of the home. The images also captured the influence of parents and online media on adolescent daily life and choices. Low motivation to consume whole grains, a common explanation for poor diet quality, was rarely mentioned. Participants proposed that adolescent whole grain consumption could be increased by raising awareness through online media, improved sensory appeal, increased availability and variety, and tailoring of products for young people. SenseCam was effective in engaging young people in dietary research and capturing data relevant to dietary choices, which is useful for future research.
Amino Acid Nutrition and Metabolism in Health and Disease
Authors: Rose Adam J.
Published: November 2019

Here an overview of the special issue “Amino acid nutrition and metabolism in health and disease” is given. In addition to several comprehensive and timely reviews, this issue had some original research contributions on fundamental research in animal models as well as human clinical trials exploring how the critical nutrients amino acids affect various traits.
Early Lifestyle Intervention for Obesity Prevention in Pediatric Survivors of Acute Lymphoblastic Leukemia
Authors: Zhang Fang Fang , Kelly Michael , Du Mengxi , Welch Jennifer J. G. , Santacruz Nadine , Rhoades Jacqueline , Kamins Christina Luongo , Dreyer ZoAnn , Scheurer Michael E.
Published: November 2019

Patients with pediatric acute lymphoblastic leukemia (ALL) experience rapid weight gain during treatment and increases in weight are maintained throughout treatment and beyond. Without prompt interventions, altered dietary and physical activity behaviors may become difficult to reverse, contributing to obesity risk long-term. Fifteen children, aged 3–9 years, diagnosed with pediatric ALL who were on maintenance therapy or within two years of treatment completion (mean BMI percentile: 70.4th) and one parent from each family, were enrolled into a 12-week lifestyle intervention delivered remotely through web-based sessions and phone calls with a lifestyle coach. Outcomes were assessed at baseline and end of the intervention. Thirteen of the 15 enrolled families (86.7%) completed the intervention. Parents reduced the “pressure to eat” feeding practice (change in mean score: −0.60, 95% CI: −1.12 to −0.07; p-value = 0.03) post intervention. Children increased the consumption of milk (0.54 serving/d, 0.02 to 1.07; p-value = 0.04) and percent of calories from protein (2.54%, 0.22 to 4.87%; p-value = 0.04) and reduced the consumption of potatoes (−0.16 serving/d, -0.30 to −0.03; p-value = 0.02). No significant changes were observed for children’s levels of physical activity, BMI, or waist circumference. Results from this pilot support the feasibility and preliminary efficacy of early lifestyle intervention among pediatric ALL survivors.
The Role of Probiotics in Nonalcoholic Fatty Liver Disease: A New Insight into Therapeutic Strategies
Authors: Meroni Marica , Longo Miriam , Dongiovanni Paola
Published: November 2019

Nonalcoholic fatty liver disease (NAFLD) encompasses a broad spectrum of pathological hepatic conditions ranging from simple steatosis to nonalcoholic steatohepatitis (NASH), which may predispose to liver cirrhosis and hepatocellular carcinoma (HCC). Due to the epidemic obesity, NAFLD is representing a global health issue and the leading cause of liver damage worldwide. The pathogenesis of NAFLD is closely related to insulin resistance (IR), adiposity and physical inactivity as well as genetic and epigenetic factors corroborate to the development and progression of hepatic steatosis and liver injury. Emerging evidence has outlined the implication of gut microbiota and gut-derived endotoxins as actively contributors to NAFLD pathophysiology probably due to the tight anatomo-functional crosstalk between the gut and the liver. Obesity, nutrition and environmental factors might alter intestinal permeability producing a favorable micro-environment for bacterial overgrowth, mucosal inflammation and translocation of both invasive pathogens and harmful byproducts, which, in turn, influence hepatic fat composition and exacerbated pro-inflammatory and fibrotic processes. To date, no therapeutic interventions are available for NAFLD prevention and management, except for modifications in lifestyle, diet and physical exercise even though they show discouraging results due to the poor compliance of patients. The premise of this review is to discuss the role of gut–liver axis in NAFLD and emphasize the beneficial effects of probiotics on gut microbiota composition as a novel attractive therapeutic strategy to introduce in clinical practice.
Grape Seed Extract Eliminates Visceral Allodynia and Colonic Hyperpermeability Induced by Repeated Water Avoidance Stress in Rats
Authors: Arie Hideyuki , Nozu Tsukasa , Miyagishi Saori , Ida Masayuki , Izumo Takayuki , Shibata Hiroshi
Published: November 2019

Grape seed extract (GSE) is rich in polyphenols composed mainly of proanthocyanidins, which are known to attenuate proinflammatory cytokine production. Repeated water avoidance stress (WAS) induces visceral allodynia and colonic hyperpermeability via toll-like receptor 4 (TLR4) and proinflammatory cytokine pathways, which is a rat irritable bowel syndrome (IBS) model. Thus, we explored the effects of GSE on repeated WAS (1 h for 3 days)-induced visceral allodynia and colonic hyperpermeability in Sprague-Dawley rats. Paracellular permeability, as evaluated by transepithelial electrical resistance and flux of carboxyfluorescein, was analyzed in Caco-2 cell monolayers treated with interleukin-6 (IL-6) and IL-1β. WAS caused visceral allodynia and colonic hyperpermeability, and intragastric administration of GSE (100 mg/kg, once daily for 11 days) inhibited these changes. Furthermore, GSE also suppressed the elevated colonic levels of IL-6, TLR4, and claudin-2 caused by WAS. Paracellular permeability was increased in Caco-2 cell monolayers in the presence of IL-6 and IL-1β, which was inhibited by GSE. Additionally, GSE suppressed the claudin-2 expression elevated by cytokine stimulation. The effects of GSE on visceral changes appear to be evoked by suppressing colonic TLR4-cytokine signaling and maintaining tight junction integrity. GSE may be useful for treating IBS.
Obesity- and Lipid-Related Parameters in the Identification of Older Adults with a High Risk of Prediabetes According to the American Diabetes Association: An Analysis of the 2015 Health, Well-Being, and Aging Study
Authors: Ramírez-Vélez Robinson , Pérez-Sousa Miguel , González-Ruíz Katherine , Cano-Gutierrez Carlos , Schmidt-RioValle Jacqueline , Correa-Rodríguez María , Izquierdo Mikel , Romero-García Jesús , Campos-Rodríguez Adriana , Triana-Reina Héctor , González-Jiménez Emilio
Published: November 2019

This study evaluated the predictive ability of 11 obesity- and lipid-related parameters, including body mass index (BMI), waist circumference (WC), waist-to-height ratio (WtHR), body roundness index (BRI), “A” body-shape index (ABSI), conicity index (C), visceral adiposity index (VAI), triglyceride-to-glucose fasting index (TyG), triglyceride-to-glucose fasting related to BMI (TyG-BMI), triglyceride-to-glucose fasting related to WC (TyG-WC), and triglyceride-to-glucose fasting related to WtHR (TyG-WtHR), to identify patients from an elderly Colombian population with a high risk of prediabetes according to the 2016 American Diabetes Association criteria. The data were obtained from the 2015 Colombian Health and Wellbeing and Aging Survey. A total of 3307 elderly Colombian individuals (aged over 60 years) were included. Anthropometric data, fasting plasma glucose, blood lipid profiles, family history, and health-related behaviors were assessed, and prediabetes was defined as a fasting plasma glucose of 100 to 125 mg/dL. The areas under the receiver operating characteristic (ROC) curves (AUCs) were calculated for each anthropometric indicator, using the prediabetes classification to identify their sensitivity and specificity, and these indicated that the prevalence of prediabetes was 25.3% in this population. After adjusting for potential confounding factors, the TyG index was strongly associated with the odds of having prediabetes in both sexes, and multivariate logistic regression analysis showed that the ORs for prediabetes increased across quartiles (p < 0.001). The TyG index was best able to identify prediabetes in either sex (AUC and optimal cut-off = 0.700 and 8.72, and 0.695 and 8.92 for men and women, respectively), suggesting that compared to the other parameters, the TyG index has the best discriminative power to predict prediabetes in the whole population. Thus, we propose the TyG index be used as a complementary marker for assessing prediabetes in older adults.
Enteral Nutrition in Patients with Inflammatory Bowel Disease. Systematic Review, Meta-Analysis, and Meta-Regression
Authors: Comeche Jose M. , Caballero Pablo , Gutierrez-Hervas Ana , García-Sanjuan Sofia , Comino Iris , Altavilla Cesare , Tuells Jose
Published: November 2019

Inflammatory bowel disease (IBD) is a chronic disease mediated by the immune system and is characterized by inflammation of the gastrointestinal tract. One of the possible treatments for this pathology is a change in the type of diet, of which enteral nutrition (EN) is one. This study is to understand how the use of EN can affect the adult population diagnosed with IBD. We conducted a systematic review, meta-analysis, and a meta-regression. On the different databases (MEDLINE, Scopus, Cochrane, LILACS, CINAHL, WOS), we found 363 registers with an accuracy of 12% (44 registers). After a full-text review, only 30 research studies were selected for qualitative synthesis and 11 for meta-analysis and meta-regression. The variables used were Crohn’s disease activity index (CDAI), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). EN has been shown to have efficacy for the treatment of Crohn’s disease and is compatible with other medicines. As for the CDAI or rates of remission, there were no differences between enteral and parenteral nutrition. Polymeric formulas have shown better results with respect to the CRP. The long-term treatment could dilute the good CDAI results that are obtained at the start of the EN treatment.
Combined Predictive Value of Extracellular Fluid/Intracellular Fluid Ratio and the Geriatric Nutritional Risk Index for Mortality in Patients Undergoing Hemodialysis
Authors: Yajima Takahiro , Yajima Kumiko , Takahashi Hiroshi , Yasuda Keigo
Published: November 2019

The ratio of extracellular fluid (ECF) to intracellular fluid (ICF) may be associated with mortality in patients undergoing hemodialysis, possibly associated with protein-energy wasting. We therefore investigated the relationship of the ECF/ICF ratio and the geriatric nutritional risk index (GNRI) with the all-cause and cardiovascular-specific mortality in 234 patients undergoing hemodialysis. Bioimpedance analysis of the ECF and ICF was performed and the ECF/ICF ratio was independently associated with GNRI (β = −0.247, p < 0.0001). During a median follow-up of 2.8 years, 72 patients died, of which 29 were cardiovascular. All-cause mortality was independently associated with a lower GNRI (adjusted hazard ratio [aHR] 3.48, 95% confidence interval [CI] 2.01–6.25) and a higher ECF/ICF ratio (aHR 11.38, 95%CI 5.29–27.89). Next, we divided patients into four groups: group 1 (G1), higher GNRI and lower ECF/ICF ratio; G2, lower GNRI and lower ECF/ICF ratio; G3, higher GNRI and higher ECF/ICF ratio; and G4, lower GNRI and higher ECF/ICF ratio. Analysis of these groups revealed 10-year survival rates of 91.2%, 67.2%, 0%, and 0% in G1, G2, G3, and G4, respectively. The aHR for G4 versus G1 was 43.4 (95%CI 12.2–279.8). Adding the GNRI alone, the ECF/ICF ratio alone, or both to the established risk model improved the net reclassification improvement by 0.444, 0.793 and 0.920, respectively. Similar results were obtained for cardiovascular mortality. In conclusion, the ECF/ICF ratio was independently associated with GNRI and could predict mortality in patients undergoing hemodialysis. Combining the GNRI and ECF/ICF ratio could improve mortality predictions.
Folate-Targeted mRNA Delivery Using Chitosan-Functionalized Selenium Nanoparticles: Potential in Cancer Immunotherapy
Authors: Maiyo Fiona , Singh Moganavelli
Published: November 2019

Systemic messenger RNA (mRNA) delivery, although still in its infancy, holds immense potential for application in cancer vaccination and immunotherapy. Its advantages over DNA transfection make it attractive in applications where transient expression is desired. However, this has proved challenging due to mRNA’s instability and susceptibility to degradation. Selenium is important for immune function and modulation, with selenium nanoparticles (SeNPs) finding a niche in biomedicine as drug delivery vehicles, owing to their biocompatibility, low toxicity, and biodegradability. In this investigation, we synthesized chitosan-coated SeNPs with a folic acid targeting moiety for Fluc mRNA delivery to cancer cells in vitro. Synthesized SeNPs were stable and well dispersed, and ranged from 59 to 102 nm in size. Nanoparticles bound and protected mRNA from RNase degradation, while exhibiting low cytotoxicity in the human embryonic kidney (HEK293), breast adenocarcinoma (MCF-7), and nasopharyngeal (KB) cells in culture. Moderate cytotoxicity evidenced in the colorectal carcinoma (Caco-2) and colon carcinoma (HT-29) cells was attributed to apoptosis induction by selenium, as confirmed by acridine orange/ethidium bromide staining. Selenium uptake studies corroborated the transfection results, where significant transgene expression was evident for the overexpressed folate receptor-positive KB cells when compared to the other cells with less or no folate receptors.
Evaluation of Electrospun Poly(ε-Caprolactone)/Gelatin Nanofiber Mats Containing Clove Essential Oil for Antibacterial Wound Dressing
Authors: Unalan Irem , Endlein Stefan J. , Slavik Benedikt , Buettner Andrea , Goldmann Wolfgang H. , Detsch Rainer , Boccaccini Aldo R.
Published: November 2019

The objective of this study was to produce antibacterial poly(ε-caprolactone) (PCL)-gelatin (GEL) electrospun nanofiber mats containing clove essential oil (CLV) using glacial acetic acid (GAA) as a “benign” (non-toxic) solvent. The addition of CLV increased the fiber diameter from 241 ± 96 to 305 ± 82 nm. Aside from this, the wettability of PCL-GEL nanofiber mats was increased by the addition of CLV. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of CLV, and the actual content of CLV was determined by gas chromatography–mass spectrometry (GC-MS). Our investigations showed that CLV-loaded PCL-GEL nanofiber mats did not have cytotoxic effects on normal human dermal fibroblast (NHDF) cells. On the other hand, the fibers exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Consequently, PCL-GEL/CLV nanofiber mats are potential candidates for antibiotic-free wound healing applications.
Cellulose Nanocrystals from Fibers of Macauba (Acrocomia Aculeata) and Gravata (Bromelia Balansae) from Brazilian Pantanal
Authors: Corrêa Ana Carolina , Carmona Vitor Brait , Simão José Alexandre , Galvani Fabio , Marconcini José Manoel , Mattoso Luiz Henrique Capparelli
Published: November 2019

Cellulose nanocrystals (CNC) were obtained from macauba and gravata fibers. Macauba (or Bocaiuva) is a palm tree found throughout most of Brazil and Gravata is an abundant kind of bromelia with 1–2m long leaves, found in Brazilian Pantanal and Cerrado. The raw fibers of both fibers were mercerized with NaOH solutions and bleached; they were then submitted to acid hydrolysis using H2SO4 at 45 °C, varying the hydrolysis time from 15 up to 75 min. The fibers were analyzed by X-ray diffraction (XRD), FTIR Spectroscopy, scanning electron microscopy (SEM) and thermal stability by thermogravimetric analysis (TG). XRD patterns did not present changes in the crystal structure of cellulose after mercerization, but it was observed a decrease of hemicellulose and lignin contents, and consequently an increase of cellulose content with the increase of NaOH solution concentration in the mercerization. After acid hydrolysis, the cellulose nanocrystals (CNC) were also analyzed by transmission electron microscopy (TEM) which showed an acicular or rod-like aspect and nanometric dimensions of CNC from both fibers, but the higher values of aspect ratio (L/D) were found on CNC obtained from gravata after 45 min of acid hydrolysis. The mercerization and subsequent bleaching of fibers influenced the crystallinity index and thermal stability of the resulting CNC, but their properties are mainly influenced by the hydrolysis time, i. e., there is an increase in crystallinity and thermal stability up to 45 min of hydrolysis, after this time, both properties decrease, probably due to the cellulose degradation by the sulfuric acid.
Influence of Filler Content and Filler Size on the Curing Kinetics of an Epoxy Resin
Authors: Zhao Yang , Drummer Dietmar
Published: November 2019

In this research, the influences of filler content and filler particle size on the flow-hardening behavior were investigated by a measuring mixer. In order to more reliably assess the observed rheological behavior, isothermal differential scanning calorimetry (DSC) measurements were employed to study the curing kinetics of the compounds. The measured data can be fitted well with Kamal-Sourour’s model modified by the diffusion correlation according to Chern and Poehlein. After that, the influence of filler content and size on the kinetic parameters are presented discussed. The results show that the ultimate glass transition temperature is significantly lower for pure epoxy resin (EP) than for compounds filled with surface-treated glass beads, which have an essential effect on the diffusion-controlled reaction at different curing temperatures. For the surface-treated glass beads used in this study, the reaction speed in the early curing stage is accelerated by increasing filler content or decreasing of filler size. In the later curing stage, the reaction speeds of compounds with higher filler content or smaller fillers reduce more quickly. The study of reaction kinetics indicates that the activation energy Ea1, Ea2, the reaction order m, and n are affected differently by varying filler content and size.
Determination of the Least Impactful Municipal Solid Waste Management Option in Harare, Zimbabwe
Authors: Nhubu Trust , Muzenda Edison
Published: November 2019

Six municipal solid waste management (MSWM) options (A1–A6) in Harare were developed and analyzed for their global warming, acidification, eutrophication and human health impact potentials using life cycle assessment methodology to determine the least impactful option in Harare. Study findings will aid the development of future MSWM systems in Harare. A1 and A2 considered the landfilling and incineration, respectively, of indiscriminately collected MSW with energy recovery and byproduct treatment. Source-separated biodegradables were anaerobically treated with the remaining non-biodegradable fraction being incinerated in A3 and landfilled in A4. A5 and A6 had the same processes as in A3 and A4, respectively, except the inclusion of the recovery of 20% of the recoverable materials. The life cycle stages considered were collection and transportation, materials recovery, anaerobic digestion, landfilling and incineration. A5 emerged as the best option. Materials recovery contributed to impact potential reductions across the four impact categories. Sensitivity analysis revealed that doubling materials recovery and increasing it to 28% under A5 resulted in zero eutrophication and acidification, respectively. Increasing material recovery to 24% and 26% under A6 leads to zero acidification and eutrophication, respectively. Zero global warming and human health impacts under A6 are realised at 6% and 9% materials recovery levels, respectively.
Automatic Hybrid Attack Graph (AHAG) Generation for Complex Engineering Systems
Authors: Ibrahim Mariam , Alsheikh Ahmad
Published: November 2019

Complex Engineering Systems are subject to cyber-attacks due to inherited vulnerabilities in the underlying entities constituting them. System Resiliency is determined by its ability to return to a normal state under attacks. In order to analyze the resiliency under various attacks compromising the system, a new concept of Hybrid Attack Graph (HAG) is introduced. A HAG is a graph that captures the evolution of both logical and real values of system parameters under attack and recovery actions. The HAG is generated automatically and visualized using Java based tools. The results are illustrated through a communication network example.
Shannon Entropy for the Hydrogen Atom Confined by Four Different Potentials
Authors: Martínez-Sánchez Michael-Adán , Vargas Rubicelia , Garza Jorge
Published: November 2019

Spatial confinements induce localization or delocalization on the electron density in atoms and molecules, and the hydrogen atom is not the exception to these results. In previous works, this system has been confined by an infinite and a finite potential where the wave-function exhibits an exact solution, and, consequently, their Shannon entropies deliver exact results. In this article, the Shannon entropy in configuration space is examined for the hydrogen atom submitted to four different potentials: (a) infinite potential; (b) Coulomb plus harmonic oscillator; (c) constant potential; and (d) dielectric continuum. For all these potentials, the Schrödinger equation admitted an exact analytic solution, and therefore the corresponding electron density has a closed-form. From the study of these confinements, we observed that the Shannon entropy in configuration space is a good indicator of localization and delocalization of the electron density for ground and excited states of the hydrogen atom confined under these circumstances. In particular, the confinement imposed by a parabolic potential induced characteristics that were not presented for other confinements; for example, the kinetic energy exhibited oscillations when the confinement radius is varied and such oscillations coincided with oscillations showed by the Shannon entropy in configuration space. This result indicates that, when the kinetic energy is increased, the Shannon entropy is decreased and vice versa.