bluuebunny/crossref_metadata_2025 huggingface dataset:
Is OEPA/COPDAC chemotherapy and PET-CT based strategy for Hodgkin lymphoma appropriate in a developing country? Lessons learned from 143 patients
Authors: Palayullakandi Achanya , Trehan Amita , Jain Richa , Kumar Rajender , Mittal B , Kapoor Rakesh , Srinivasan Radhika , Kakkar Nandita , Bansal Deepak
Published: January 2024

Background: ABVD regimen for Hodgkin lymphoma (HL), disfavoured in
high-income countries, is popular in low-middle-income countries (LMIC).
The feasibility/safety data for ‘non-ABVD’ protocols from LMIC is
limited. Procedure: The retrospective study was conducted in a single
center in India. Euronet-PHL-C1-based protocol was administered during
2010-19. A PET-CT was performed at diagnosis and following
OEPA-course-2. Radiotherapy was administered for inadequate
PET-response. Results: During the 10-year-period, 143 patients with HL
were treated. The mean-age was 7.8±2.5 years. Bulky-disease was observed
in 82 (59%). Treatment-abandonment was recorded in 13 (9.1%). The
median follow-up duration was 46.4 months. An inadequate PET-response
was observed in 41/118 (34.7%). Radiotherapy was administered to 23/41
(56.1%). There was a protocol violation of replacing radiotherapy in 12
(29.3%) patients with 2-courses of COPDAC. Sixty-nine episodes of
febrile-neutropenia were observed in 54 patients. TRM was observed in 7
(5.3%). The majority of episodes of febrile-neutropenia (61%) and TRM
(86%) were following OEPA-course-1. The 4-year overall-survival (OS)
and event-free survival (EFS) were 93.5±2.2% and 86.2±3.4%,
respectively. Nine (6.3%) patients relapsed. The survival compared
favorably with 5-year-EFS (77.7%) of patients who received ABVD/COPP in
the center in the past. Bulky-disease lacked association with inadequate
PET-response (p=0.800) or relapse (p=1.000). Conclusions: OEPA/COPDAC
regimen and response assessment by PET-CT permitted therapy reduction,
including radiotherapy. The survival (4-year OS: 93.5±2.2%) was
excellent, with a low relapse (6.3%). Febrile neutropenia and resultant
TRM (5.3%) are concerning and occurred frequently following
OEPA-course-1. The support system for managing febrile neutropenia
should be optimized for administering OEPA in LMIC.
Retrospective study of off-label use of Tocilizumab: a single-center experience
Authors: Liu Rongji , Gao Yiqiang , Hu Yang , Zuo Wei , Zhang Bo
Published: January 2024

To investigate the prevalence of tocilizumab off-label prescribing in
outpatient department of a tertiary hospital and evaluate the
rationality of these off-label prescriptions by looking through
scientific evidences. Setting Peking Union Medical College Hospital in
Beijing in China. Method Retrieving one year tocilizumab related
prescriptions from hospital electronic information system. The standard
of evaluating the on-label or off-label prescription according to
whether the indication approved by China. Logistic regression was
applied to explore predictive variables. All of the literature source
acquired from Up-To-Date, Micromedex database, PubMed, Embase, and
clinical guidelines. Main outcome measure The percentage of off-label
use, influence factors identified by logistic regression, the
rationality of off-label use. Conclusion according to our analysis, the
majority of off-label indications of tocilizumab in our hospital have
evidence support. But some indications were supported by low grade
scientific evidence, more studies are required to confirm the clinical
values. For the indication that has no evidence support, we suggest the
administration should strength supervision.
Optimal angular sampling for 3D volume matching and faster fitting of 3D structures into cryogenic electron microscopy data
Authors: Titarenko Valeriy , Roseman Alan
Published: January 2024

A numerical algorithm to generate a set of optimal 3D rotations is
presented. A list is created, with each new rotation found in a way to
minimise the gap in angular coverage for a global angular search or a
local angular refinement problem. Efficiency can be gained by combining
rotations in groups when fitting molecular density representations to an
experimental electron density map in cryo electron microscopy, and this
algorithm also works for those combined rotations. Lists of optimal
rotations written as quaternions and code to generate those lists are
provided. The maximum gap in the angular coverage decays as a
reciprocal-cubic function of the number of rotations.
Persistence, extinction and blowup in a generalized logistic model with impulses and regional evolution
Authors: Xu Haiyan , Lin Zhigui , Santos Carlos
Published: January 2024

To explore the impacts of regional evolution and impulses on the
persistence or extinction of species, a generalized logistic model with
impulses in an evolving domain is proposed and researched. Firstly, the
ecological reproduction index, which is regarded as a threshold value,
is introduced and characterized. Secondly, in the case of monotone or
non-monotone impulsive function, the asymptotic behavior of population
is fully investigated and the sufficient conditions for the solution to
persist, be extinct or blow up are given. Finally, numerical simulations
results indicate that whatever impulse is, larger periodic evolution
rates are more favorable for species. However, impulsive harvesting has
a negative impact on persistence of species, while birth pulse admits a
positive impact and even results in blowup.
The existence results for Hilfer-Katugampola fractional stochastic differential equations with non-instantaneous impulsive
Authors: Gou Haide
Published: January 2024

This paper is concerned with sufficient conditions for existence
solutions of noninstantaneous impulsive Hilfer-Katugampola fractional
differential equations of order 1/2 < α < 1 and
parameter 0 ≤ β ≤ 1 with fractional Brownian motion (fBm) and poisson
jumps, by using M¨onch fixed point theorem, fractional calculus and
stochastic analysis. At last, as an application, an example is presented
to illustrate the abstract results.
PCOS: a chronic disease that fails to produce adequately specialized pro-resolving lipid mediators (SPMs).
Authors: Regidor Pedro-Antonio , Rosa Xavier de la , Mueller Anna , Sailer Manuela , Santos Fernando Gonzalez , banzo Rafael Gracia , Rizo Jose Miguel
Published: January 2024

The present study aimed to establish lipid mediator profiles of PCOS
patients compared to healthy women to identify differences in their
resolutive and pro-inflammatory lipid parameters. Main measures:
Pro-inflammatory lipid mediators and their precursor AA; SPMs, their
precursors. The level of pro-inflammatory parameters was significantly
higher in PCOS-affected women.
Evaluation of electrophysiological characteristics and ventricular synchrony: An Intra-patient-controlled Study during His-Purkinje conduction system pacing (HPCSP) versus right ventricular pacing(RVP)
Authors: Chen Xueying , JIN QINCHUN , Wang Yanan , ZHOU XIAOLAN , CHEN YUFEI , Wang Jing-Feng , Qin Sheng-Mei , Bai Jin , Wang Wei , Liang Yixiu , Chen Haiyan , SU YANGANG , GE JUN-BO
Published: January 2024

Objectives: To compare electromechanical ventricular synchrony when
pacing from different sites including right ventricular apex pacing
(RVAP), right ventricular septum pacing (RVSP), His Bundle pacing (HBP),
left bundle branch pacing (LBBP) and RVSP during unipolar pacing from
the ring electrode of LBBP lead (RVSPring ) in each patient. Methods:
Twenty patients with complete atrioventricular block (AVB) indicated for
dual-chamber pacemaker implantation were included. Unipolar pacing at
different sites including RVAP, RVSP，HBP，LBBP and RVSPring were
successively performed. Pacing characteristics and echocardiogram
parameters were compared among intrinsic rhythm and pacing at different
sites. Results: Similar to HBP(114.84 ±18.67ms), narrower paced QRSd was
found in LBBP(116.15±11.60ms) versus RVSPring(135.11±13.68ms),
RVSP(141.75±14.08ms) and RVAP(158.15±21.41ms)(p＜0.001). LBBP showed
comparable pacing parameters to RVAP or RVSP and were significantly
better than HBP, with maintained cardiac function. TS-12-SD was
significantly improved in LBBP(27.00±21.53ms) than RVAP(54.05±34.21ms,
p=0.004) and RVSP(47.56±33.26ms, p=0.029) but similar to
HBP(37.05±26.24ms, p=0.283) or RVSPring(42.16±26.19ms, p=0.107).
Negative values of interventricular mechanical delay(IVMD) were only
identified in LBBP(-20.16±18.47ms), significantly different from
RVAP(33.68±30.98ms), RVSP (21.68±22.02ms), HBP (4.74±19.04ms) and
RVSPring(14.56±26.76ms(all p＜0.001). Using Pearson’s analysis, Sti-LVAT
was positively corelated with QRS, IVMD, TS-12-SD, LVEDV and LVESV while
a negative relationship was identified for LVEF. Conclusions: Similar to
HBP, LBBP achieved better electrical and mechanical left ventricular
synchrony than conventional RV pacing. For interventricular synchrony,
only LBBP initiated earlier LV activation than RV among these pacing
strategies, in consistent with the RBBB pattern of paced QRS during
LBBP.
A naturally evolved mutation (Ser-59-Gly) in glutamine synthetase confers glufosinate resistance in plants
Authors: Zhang Chun , Yu Qin , Han Heping , Yu Chaojie , Nyporko Alexey , Tian Xingshan , Beckie Hugh , Powles Stephen.B.
Published: January 2024

Glufosinate is an important and widely used non-selective herbicide
active on a wide range of plant species. Resistance evolution to
glufosinate in weedy plant species (including the global weed Eleusine
indica) is underway. Here, we uncover the molecular basis of target-site
glufosinate resistance in Eleusine indica. Full-length E. indica
glutamine synthetase (GS) iso-genes (EiGS1-1, 1-2, and 1-3 and EiGS2)
were cloned, and expression of EiGS1-1 and EiGS1-2 was higher than
EiGS2. A novel point mutation resulting in a Ser-59-Gly substitution in
EiGS1-1 was identified in glufosinate-resistant (R) plants. Rice calli
and seedlings transformed with the mutant EiGS1-1 gene were resistant to
glufosinate. Purified yeast recombinant mutant EiGS1-1 was more tolerant
to glufosinate than the wild type (WT) variant. These transgenic results
correlate with a more glufosinate-R GS in the crude tissue extract of R
versus susceptible E. indica plants. Structural modelling of the mutant
EiGS1-1 revealed that the Ser-59 is not directly involved in glufosinate
binding but in contact with some important binding residues (e.g.
Glu-297) and especially with Asp-56 that forms an intratoroidal contact
interface. Importantly, the same Ser-59-Gly mutation was also found in
geographically isolated glufosinate-R populations from Malaysia and
China, suggesting parallel evolution of this resistance mutation.
Existence and asymptotic behavior of ground state solutions for quasilinear Schr\”{o}dinger equations with unbounded potential
Authors: Xue Yanfang , Zhong Xiao-Jing , Tang Chunlei
Published: January 2024

We study the existence of standing wave solutions for the quasilinear
Schr\”{o}dinger equation with the critical exponent
and singular coefficients. By applying the Mountain Pass Theorem and the
Concentration Compactness Principle, we get a ground state solution.
Moreover, the asymptotic behavior of the ground state solution is also
obtained.
Successful Treatment of Refractory Graft Versus Host Disease with Ruxolitinib in a Child After Autologous Stem Cell Transplantation
Authors: Eker Nurşah , Tas Burcu Tufan , Dogru Omer , senay emel , Tokuc Gulnur , Koc Ahmet
Published: January 2024

Autologous hematopoietic stem cell transplantation(AHSCT) is an
increasingly used curative treatment for some solid tumors in children.
Instead of allogeneic transplantation, the risk of developing graft
versus host disease (GvHD) is much lower after AHSCT. Although the
clinical findings of auto-GVHD are mild and self-limited in most cases,
rare cases may be severe and need intensive immunosuppressive treatment.
Here, we present a case who underwent autologous HSCT due to relapsed
neuroblastoma, developed steroid-refractory GvHD after AHSCT, and
achieved remission using ruxolitinib. A 12 years old female patient was
diagnosed with relapsed neuroblastoma. After metaiodobenzylguanidine
treatment, AHSCT was performed, and the status of the disease was a very
good partial response at the time of transplantation. Our patient was
diagnosed with severe and steroid-refractory GvHD with skin involvement
after AHSCT. We used ruxolitinib with extracorporeal photopheresis
because of the essential side effects of the other drugs and got a very
good response. Over the following five months, there was no recurrence
of GvHD. She was in complete remission of neuroblastoma after two years
of AHSCT. It is crucial to keep in mind that GvHD may develop after
AHSCT. Ruxolitinib is an effective treatment for GvHD also after AHSCT.
EFFECT OF INTRODUCED NON-NATIVE FISH SPECIES ON ECOSYSTEM SERVICES ON LAKE KARIBA FISHERY
Authors: Pojana Mebbah , Jere Wilson , Chimatiro Sloans
Published: January 2024

The purpose of the study was to assess the effect of introducing
non-native fish species (Limnothrissa miodon, Oreochromis niloticus and
Cherax quadricarinatus) have on ecosystem services of the Lake Kariba
fishery. Three hundred and seventy-seven (377) respondents were picked
randomly from the 3 strata (I, II and IV) of the lake and were
interviewed using a questionnaire. Focus Group Discussion (FGD) and
interviews with headmen/women, experts, government leaders and
processors/exporters were also done to strengthen and validate the
results. Analysis of data was done by cross-tabulation using chi-square
analysis using SPSS version 16. Microsoft Excel was used to produce
outputs, which was done based on defined themes. Responses from FGD and
key informants were analyzed using classical content analysis. The study
revealed that although there has been an ecological impact on the Lake
Kariba fishery, introduced non-native species have had a positive effect
on ecosystem services through improved peoples’ livelihoods brought
about by improved household food security, improved nutrition,
employment creation and overall contribution to the Gross Domestic
Product (GDP) of the country. Around 59.42% of the respondents showed
an affirmative response that introduced non-native fish species have a
positive response on their lives. It’s therefore concluded that despite
the ecological effects, introduced species have a positive effect on
livelihoods through improved food security, employment creation, income
generation and overall contribution to Zambia’s GDP. Keywords:
Non-native species, Ecosystem services, Oreochromis niloticus,
Limnothrissa miodon, Cherax quadricarinatus, Ecosystem, Lake Kariba.
Stochastic rumors on random trees
Authors: Junior Valdivino , Rodríguez Pablo Martín , Speroto Adalto
Published: January 2024

The Maki-Thompson rumor model is defined by assuming that a population
represented by a graph is subdivided into three classes of individuals;
namely, ignorants, spreaders and stiflers. A spreader tells the rumor to
any of its nearest ignorant neighbors at rate one. At the same rate, a
spreader becomes a stifler after a contact with other nearest neighbor
spreaders, or stiflers. In this work we study the model on random trees.
As usual we define a critical parameter of the model as the critical
value around which the rumor either becomes extinct almost-surely or
survives with positive probability. We analyze the existence of
phase-transition regarding the survival of the rumor, and we obtain
estimates for the mean range of the rumor. The applicability of our
results is illustrated with examples on random trees generated from some
well-known discrete distributions.
Development of Evidence-based Core Information Guideline for Health Communication of Tobacco Control–the Effect of Smoking on Risks of Female Disease: A Delphi Survey
Authors: Liu Jin , MAO Huijia , HAO Yunyi , CAO Meiling , CHEN Leilei , QI Fei , ZHANG Ying , JIN Xuezheng , WU Yibo
Published: January 2024

Objective To form the Evidence-based Core Information Guideline for
Health Communication of Tobacco Control-the Effect of Smoking on Risks
of Female Disease and reach a consensus based on Delphi method. Design A
Delphi survey Setting The International Practice Guidelines Registry
Platform (http: //www.guidelines-registry.org/.). The registration
number is IPGRP-2020CN072. Sample 32 multidisciplinary experts including
clinicians, nurses, health communication staff, methodologists,
epidemiologists, pharmacists, health economics experts, audience
representatives and government managers. Methods From May 2020 to March
2021, the preliminary studies of the guideline including collection and
grade of evidence, survey of public preference and value, etc were
completed. Then the Delphi survey involving panel of experts was
undertaken to grade the strength of recommendation integrated with
research evidence, clinical experience, patients’ value and preference
in order. Results After two rounds of Delphi survey, 47 of 51 core
information has reached a consensus (Consensus rate: 92.17%, 12 strong
recommendations and 35 weak recommendations). The positive coefficients
of experts are 100% (32/32) and 84.38% (27/32) respectively. The
coefficient of variation (CV) of each core information is less than
0.35. Conclusion Evidence-based Core Information Guideline for Health
Communication of Tobacco Control-the Effect of Smoking on Risks of
Female Disease has good authority, representativeness and reliability,
which will be of great significance to improve the effectiveness of
health communication of tobacco control and provide structured
suggestions for future guidelines research. Key words Tobacco
control；Smoking；Smoking cessation；Health communication；Female
disease; Delphi Technique
Comparative characterization of mitogenomes from five orders of elasmobranch parasites indicate that total evidence analysis is superior to single genes for cestodes (Cestoda: Tapeworms)
Authors: Trevisan Bruna , Machado Denis , Lahr Daniel J. G. , Marques Fernando
Published: January 2024

The recognized potential of using mitogenomics in phylogenetics and the
more accessible use of high-throughput sequencing (HTS) offer an
opportunity to investigate groups of neglected organisms. Here, we
leveraged HTS to execute the most comprehensive documentation of
mitogenomes for cestodes based on the number of terminals sequenced. We
adopted modern approaches to obtain the complete mitogenome sequences of
86 specimens representing five orders of cestodes (three reported for
the first time: Phyllobothriidea, “Tetraphyllidea” and
Trypanorhyncha). The complete mitogenomes provided represent an increase
of 41% of the mitogenomes available for cestodes (61 to 147) and an
addition of 33% in the representativeness of the cestode orders. The
complete mitochondrial genomes are conserved, circular, encoded in the
same strand, and transcribed in the same direction, following the
pattern observed previously for tapeworms. Their length varies from
13,369–13,795 bp, containing 36 genes in total. Except for the
Trypanorhyncha specimen, the gene order of the other four cestode orders
sequenced here suggests that it could be a synapomorphy for the
acetabulate group (with a reversion for taenids). Our results also
suggest that no single gene should be used as a molecular marker alone
since none of them could tell all the evolutionary history in the
mitogenome and that cestodes phylogenies should be improved with
phylogenetic analysis conducted under a total evidence framework. The
characterization of the new mitochondrial genomes is the first step to
provide a valuable resource for future studies on the evolutionary
relationships of these groups of parasites.
Setting up the stillbirth registration system and investigating the related causes in Iran in selected hospitals: Observational cohort study
Authors: Moradi-Lakeh Maziar , Khalili Narjes , Heidarzadeh Mohammad , Habibelahi Abbas , Larijani Samaneh Saghafian , Motamedi Mahnaz , Kashanian Maryam , Taraghi Fatemeh
Published: January 2024

Abstract: Objective: Determine the related causes of stillbirth and
reduce the unexplained cases Design: Observational cohort study Setting:
14 selected hospital from 12 provinces (14 cities) of Iran Population: A
total of 105,562 births and 762 registered stillbirths at 22 completed
weeks of gestation or more Methods: Iranian Maternal and Neonatal
Network (IMaN) registers information about almost all births (live &
dead) around the country, but this network does not collect data about
stillbirth causes. In this study, we developed the stillbirth evaluation
protocol with experts’ cooperation, and we designed forms for the
stillbirth registration system electronically. Then we trained related
individuals in 14 selected hospitals around the country. After a year,
we extracted, analyzed, and, based on the Relevant Condition of Death
Classification (ReCoDe), interpreted the collected data. Main outcome
measures: Related causes of stillbirth Results: In 742 registered
stillbirth cases in 14 selected hospitals, the relevant causes were
identified in 65.4% of cases, while 34.6% of cases remained
unclassified. The most frequent relevant conditions were fetal (33.2%),
maternal (9.1%), amniotic fluid (8.8%), placenta (7.7%), and
umbilical cord (6.2%). Conclusions: Our registration decreased the
percentage of stillbirth with an unexplained cause from about 70% to
34.6%. Funding: Iran University of Medical Sciences Key words:
stillbirth, cause of stillbirth, Iran Tweetable abstract: The most
frequent relevant causes of stillbirth in Iran were fetal, maternal, and
amniotic fluid conditions.
Probing the synergistic ratio of HPO/AtCPR to improve (+)-nootkatone production in Saccharomyces cerevisiae
Authors: Cha Yaping , Li Wen , Wu Tao , You Xia , Chen Hefeng , Zhu Chaoyi , Zhuo Min , Chen Bo , Li Shuang
Published: January 2024

(+)-Nootkatone is one type of expensive sesquiterpene aroma found in
grapefruit peel and heartwood of yellow cedar. It could be used as food
additives, perfume, insect repellent and therefor its highly efficient
production is greatly requested. However, the low catalysis efficiency
of membrane-anchored cytochrome P450/P450 reductase system (HPO/AtCPR)
was the main challenge and limited the production of (+)-nootkatone. We
developed an effective high-throughput screening system based on the
cell-wall destruction to probe the optimal ratio of HPO/AtCPR, which
achieved a 2-fold elevation in (+)-valencene oxidation in S.
cerevisiae. In combination with the introduction of valencene synthase
from Callitropsis nootkatensis and alcohol dehydrogenase form
Pichia pastoris, an engineered strain was constructed to realize
de novo (+)-nootkatone production. Then a series of engineering
strategies, including knockout of the transcription factor (ROX1),
down-regulating the squalene synthase enzyme (EGR9), overexpression of a
truncated version of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase
(tHMG1) and type III membrane protein (ICE2), were applied to generate
PK2RI-AtC/HA. In bi-phasic fed-batch fermentation, a
maximum yield of 3.73 and 1.02 g/L for valencene and (+)-nootkatone were
achieved. The dramatically improved performance of the constructed
S. cerevisiae provides an excellent approach for economical
production of (+)-nootkatone from glucose.
Renal consequences of the novel coronavirus disease 2019 (COVID-19) and hydrogen sulfide as a potential therapy
Authors: DUGBARTEY GEORGE
Published: January 2024

The novel coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is
a global pandemic which is primarily considered a respiratory illness.
However, emerging reports show that the virus exhibits both pulmonary
and extra-pulmonary manifestations in humans, with the kidney as a major
extra-pulmonary target due to its abundant expression of
angiotensin-converting enzyme 2 and transmembrane protease serine 2,
which facilitate entry of the virus into cells. Acute kidney injury has
become prevalent in COVID-19 patients without prior any history of
kidney dysfunction. In addition, the virus also worsens kidney
conditions and increases mortality of COVID-19 patients with
pre-existing chronic kidney disease, renal cancer, diabetic nephropathy,
end-stage kidney disease as well as dialysis and kidney transplant
patients. In the search for antiviral agents for the treatment of
COVID-19, hydrogen sulfide (H2S), the third established member of
gasotransmitter family, is emerging as a potential candidate, possessing
important therapeutic properties including antiviral, anti-inflammatory,
anti-thrombotic and antioxidant properties. A recent clinical study
revealed higher serum H2S levels in survivors of COVID-19 pneumonia with
reduced interleukin-6 levels compared to fatal cases. In this review, we
summarize the global impact of COVID-19 on kidney conditions and discuss
the emerging role of H2S as a potential COVID-19 therapy.
A Survey on the Potential Acceptance, Knowledge and Attitude Regarding the COVID-19 Vaccine: A Cross Sectional Study in Assam, North-East, India.
Authors: Goswami lalit , Alom sarowar , sultana farzin , sarma barnali , das manas , kalita jogen
Published: January 2024

Aim: Along with Assam, India’s mass vaccination drive is witnessing a
low turnout situation. This study aims to identify limiting factors to
the vaccination effort, attitude towards COVID-19, and attitude towards
the vaccine. During the survey, doubts of the participants on COVID-19
vaccine were also addressed. Subject and Methods: It was a
cross-sectional web and physical interview based survey using validated
questionnaire developed in the google form to assess 695 voluntary
participants of Assam, India. Results: 52.09% of the participants were
acceptable to COVID-19 vaccine, and 21.30% participants were
vaccinated. About 41.87% of the respondents were hesitating to get
vaccinated, and 10.64% of the participants refused. Education shows
inverse relation to vaccine hesitancy. Interestingly, housewives were
found to be hesitating the most (OR = 5.113, 95CI% = 1.347-19.399, p =
0.016). Retired person (OR = 4.061, 95CI% = 1.464 – 11.262, p = 0.007)
and 60 plus age group (OR = 0.373, 95CI% = 0.153 – 0.911, p = 0.031)
show a refusal to the vaccine. 99.7% of the participants have taken at
least one preventive measures depicts there is a positive attitude
towards COVID-19. Digital knowledge is one of the important aspects
associated with participants getting vaccinated (p > 0.05).
Conclusion: There are concerns about COVID-19 vaccine safety and its
efficacy. Government-sponsored awareness programme or non-governmental
organization (NGO) can launch awareness programmes to shed light on
critical areas to eliminate vaccine-related doubts. Key sources of
information can be used to channel authentic information to narrow down
the anti-vaccine folks.
Paternal germline mosaicism of a missense mutation in ELANE in a Chinese male with severe congenital neutropenia
Authors: Ying Yingfen , Ye Jinbin , Chen Yaming , Chen Yilu , Lu Xiaosheng , Gu Feng , Pan Deng , Zhao Junzhao
Published: January 2024

Background: Severe congenital neutropenia (SCN) is an autoimmune disease
clinically characterized by persistent and severe neutropenia. It is
typically caused by a heterozygous mutation in the ELANE gene, which
encodes neutrophil elastase. Procedure: Using one 3-year-old male
diagnosed with severe congenital neutropenia, we aimed to assess the
clinical and genetic characteristics of SCN caused by ELANE mutations
and test whether the mutation can be corrected by CRISPR/Cas9-mediated
gene editing. The proband underwent extensive clinical assessments, with
exome sequencing and bioinformatics analysis to identify pathogenic
genes. In addition, Sanger sequencing was used to verify the pedigrees.
The cell line, 293-ELANE, harboring ELANE mutation was generated by
lentiviral transduction and selection with puromycin from HEK-293. And
the mutation was corrected by CRISPR/Cas9-mediated homology-directed
repair (HDR). Results: The ELANE gene test in the proband unveiled a
heterozygous de novo missense mutation: c. 248T>A (p.V83D),
which is not detected in his asymptomatic parents with peripheral blood
samples. Surprisingly, we found that 46.01% of his father’s sperm cells
had the same mutation. These results demonstrated that the proband
inherited the ELANE mutation from his father, who had a normal
neutrophil count but was germline mosaic. In this study, the highest
repair efficiency of CRISPR/Cas9-mediated HDR for 293-ELANE is 4.43%.
Conclusions: We identified a missense mutation (p.V83D) in ELANE causing
SCN, the first report on paternal semen mosaicism of an ELANE mutation.
Our study also paves the way for preimplantation genetic diagnosis (PGD)
based on ELANE mutation prevention and clinical treatment of congenital
disabilities.
E-Training Hazard Content for Healthcare Workers in Emergency Departments.
Authors: Norman Fatimah , hajar siti , Siraj Saedah
Published: January 2024

Abstract: Background: Emergency department employees are exposed to a
significant number of sick patients and a wide range of dangerous
situations in a congested emergency and trauma department with a high
patient turnover. Their understanding of occupational dangers particular
to their professions, as well as administrative processes for mitigating
such risks, is important to their safety. As a result, the goal of this
research is to look into an e-Training material for healthcare workers
about emergency room dangers. This research was founded on Recognizing
Hazard theory and E-learning theory. Methods: The Fuzzy Delphi Method
(FDM) was used in the study, which was performed among 16 experts to
select a list of material on the e-training content of risks. The basic
content elements were established by a survey of literature and
interviews with specialists. The items were chosen based on their
replies to an FDM survey questionnaire according to a seven-Likert
linguist scale. The ‘threshold’ value (d) was used to determine the
expert’ consensus for the questionnaire, whereas defuzzification (Amax)
values recorded the experts’ recommendations. Results: Only 14 contents
were accepted based on expert consensus. This resulted in the conclusion
that the content finding demonstrated a high degree of acceptability
among experts, and the finding suggested the necessity of undertaking
curriculum design. Conclusion: Further research can concentrate on
additional online training implementation curriculum packages that might
be designed for unique learner needs, such as post-natural disaster
training, rookie health care worker training, and others.
Influence of Vestibular Disorders on Tilt Perception and Short Term Memory
Authors: Ochiai Atsushi , Kaga Kimitaka
Published: January 2024

Introduction: Spatial orientation refers to the fact that the brain
integratively recognizes its own position, posture and motion in space
through several sensory systems. Vestibular, visual and somatosensory
inputs with various motion are constantly integrated in central nervous
system to determine the spatial orientation. Among the various
parameters of spatial orientation, there has been no study about tilt
perception and the memory of tilt perception. Methods: The following
subjects participated in these experiments; normal volunteers under 65
years of age (Control group) and bilateral no response to the Caloric
test (Bilateral group). Procedure was measurement of the short term
memory of tilt perception that were reproduction of 0°and reproduction
of right by 5°and left by 5°with improved electric goniometer. Results:
Control group: There were no significant differences in the time course,
or right and left direction of tilt for any of the age groups. Bilateral
group: There were no significant differences in any of the tasks in the
procedures between the control group and the bilateral group.
Conclusion: Although tilt perception is formed from the vestibular and
somatosensory input, it became clear that the vestibular are less
important than the somatosensory input, because the bilateral group
could also successfully remember tilt positions. It also is clear that
such information is remembered for at least a short period of time.
Predicting the loss to follow-up (LTFU) of AIDS patients in China using a recency-frequency (RF) model
Authors: Li Min , Wang Qunwei , shen yinzhong
Published: January 2024

Objective: This study constructs and verifies a recency-frequency (RF)
model for predicting the loss to follow-up (LTFU) in acquired
immunodeficiency syndrome (AIDS) patients in China. Methods: (1) We
exported the full data of AIDS outpatients in the research unit from
August 2009 to September 2020 as the observation dataset, from September
to December 2020 as the prediction dataset. (2) We cleaned the data to
obtain one data element per person. The classic
recency-frequency-monetary (RFM) model was expanded into four models
(RFM, RF, RFL and RFML, L: length of treatment cycle), using the k-means
clustering algorithm for model construction and C5.0 decision tree
algorithm for verification to the best predictive model. (3) After, the
best predictive model was subjected to two rounds of k-means clustering.
In the retained data were randomly divided into a training set (70%)
and a testing set (30%). (4) The artificial neural network algorithm
was used to predict the LTFU of AIDS patients and the confusion matrix
was used to evaluate the performance. Results: (1) The observation
dataset included 16,949 elements and the prediction dataset had 10,748
elements. (2)In the best predictive model, an RF model with a quality of
0.82, the observation dataset was grouped into 3 patient types by 2
rounds of k-means clustering, and the accuracy rate was 99.99%. (3) A
retained of 13,799 data elements were retrained and randomly binned into
a test set (70%) and a verification set (30%), the accuracy rate was
100.0%. (4) LTFU of AIDS patients was predicted in the prediction set
with a correct rate of 99.89% and the accuracy and precision were
85.41% and 99.76%, respectively. Conclusion: The RF model verified
using a neural network algorithm is effective, reliable and feasible to
predict the LTFU of AIDS patients in China.
EVALUATION OF THE GAS EXCHANGE CHARACTERISTICS OF SELECTED PLANT SPECIES OF UNIVERSITI TUN HUSSEIN ONN MALAYSIA (UTHM)
Authors: Yunusa Audu , Cuevas Alona , Mohammed Jibrin , Idris Aisha
Published: January 2024

The maximum carboxylation rate (Vcmax) of Rubisco and the maximum
electron transport rate (Jmax), light compensation point (LCP), light
saturation point (LSP), maximum photosynthesis (Amax), and apparent
quantum yield (Aqy) are gas exchange characteristics that are derived
from the carbon dioxide (CO2) and light response curves. This
characteristics can be affected by the level of CO2 and light received
by the plant. Moreover, the characteristics determines the
photosynthetic capacity of the plant. The present study evaluates the
gas exchange characteristics of selected plant species of UTHM.
Photosynthetic carbon dioxide (A\Ci) and light (A/Q)
response curves were measured using portable photosynthesis system
(LICOR). The results shows that both A/Ci and A/Q curves increases as
CO2 and light increases but got to a certain point where the curves
become saturated. Spathodea campanulata was having the highest Vcmax
(52.14±0.005 µmolCO2 m-2s-1), Jmax (104.461±0.011 µmolCO2 m-2s-1) and
Aqy (0.072±0.001 mol CO2 mol-1 photons). The highest LCP was observed in
Rhaphis excelsa (69.60±0.067 µmol photons m-2s-1) while the highest LSP
was recorded for Costus spicatus (1576.69±0.173 µmol photons m-2s-1). It
was concluded that the plants need high light intensity and CO2 to
achieve their maximum assimilation rate
Going deeper into the molecular ecology of the Southwest Atlantic Caretta caretta (Testudinata: Cheloniidae), what microsatellite reveals to us?
Authors: LUDWIG SANDRA , Amorim Lais , Barcelos Ana , Guimaraes Paula , Medeiros Luciana , Erickson José , vargas sarah
Published: January 2024

The loggerhead (Caretta caretta) is a cosmopolitan sea turtle species
that are listed as vulnerable by IUCN and endangered in Brazil. In 2015,
the C. caretta Southwest Atlantic subpopulation (SWA) was impacted by
the Fundão dam break’ (FFDT) ore tailings plume and heavy metals,
reaching the Espírito Santo coast (ES). The SWA is a great regional
management unit (RMU) of C. caretta worldwide due to distinctive mtDNA
lineage promoted by recent radiation within the Atlantic-Mediterranean
region, however, due to the low resolution of mtDNA, the population
structure of C. caretta SWA has been shallowly studied. Here, we
recovered the genetic diversity and evaluated the population structure
of the C. caretta SWA, using both mtDNA and 15 nDNA microsatellite loci,
along with four nesting areas, and over 16 years enclosing the periods
before and after the FFDT. We detected complex genetic dynamics that
reflect on genetic composition at a population-individual level and
revealed a spatial-temporal pattern that may be related to its
philopatric behavior and relatedness. Further, we detected a genetic
signature for the females of the ES nesting area that could be a
consequence of recent demographic events and/or environmental responses.
Thus, this study deeply evaluated the genetic diversity and provide a
fine-scale resolution of the population structure of C. caretta SWA and
the effects of environmental accidents (like the FFDT) on its life
history. Lastly, for conservation purposes, continuous monitoring is
needed to deeply evaluate if the FFDT has considerably impacted its
evolutionary dynamics in the next generations.
CARDIAC MYXOMA: CHARACTERIZATION BY 3D-TRANSILLUMINATION RENDERING IN THE ERA OF MULTIMODAL IMAGING. A CASE REPORT.
Authors: Barquín Raquel Pérez , Cuesta Jose Maria , Canedo Cesar Rodríguez , Castro Adrián Margarida de , Zamacola Jon Zubiaur , Lozano David Serrano , Ortega Jose María Gómez , Ruisanchez Cristina , Navarro Jesús Zarauza
Published: January 2024

The heart is an infrequent location for primary tumors. When they
appear, they usually emerge as myxomas (slow growth bening forms). We
present a 68-year-old man who was discovered of a left atrial mass in a
routinary echocardiography. A transesophageal test was then performed to
better study the finding. We highlight “Transillumination”as a new
technology which processes the 3D image by operating with the image’s
enlightenment. It provides a better tissue characterization, allowing a
superior description of the mass, in this case. The bulk was surgically
removed, and its histological analysis established the diagnosis of a
myxoma.
Straightforward synthesis of quinazolin-4(3H)-ones via visible light-induced condensation cyclization
Authors: Sun Wuji , Ma Xue , Pang Yuqi , Zhao Lifeng , Zhong Qidi , Liu Chunyan , Fan Qiangwen
Published: January 2024

A green, simple and efficient method is developed for the synthesis of
quinazolin-4(3H)-ones via visible light-induced condensation cyclization
of 2-aminobenzamides and aldehydes under visible light irradiation. The
reaction proceeds using fluorescein as photocatalyst in the presence of
TBHP without the need for metal catalyst. In addition, this reaction
tolerates a broad scope of substrates and could afford a variety of
desirable products in good to excellent yields. Thus, the present
synthetic method provides a straightforward strategy for the synthesis
of quinazolin-4(3H)-ones.
Symmetry and Nonexistence of Positive Solutions for a fractional Laplacion System with coupled terms
Authors: Zhang Rong
Published: January 2024

In this paper, we study the problem for a nonlinear elliptic system
involving fractional Laplacion: $$ \begin{cases}
\
(-\Delta)^{\frac{\alpha}{2}}u=|x|^{\gamma}u^{p}v^{q+1},\\
\
(-\Delta)^{\frac{\beta}{2}}v=|x|^{\tau}u^{p+1}v^{q},
\end{cases} $$ where
$0<\alpha,\beta<2,$
$p,q>0\ and\
\max\{p,q\}\geq1,$
$\alpha+\gamma>0,\beta+\tau>0,$
$n\geq2$. First of all, while in the subcritical case,
i.e.
$n+\alpha+\gamma-p(n-\alpha)-(q+1)(n-\beta)>0$,
$n+\beta+\tau-(p+1)(n-\alpha)-q(n-\beta)>0$,
we prove the nonexistence of positive solution for the above system in
$\mathbb{R}^{n}$. Moreover, though
$Doubling\ Lemma$ to obtain the singularity estimates
of the positive solution on bounded domain $\Omega$. In
addition, while in the critical case, i.e.
$n+\alpha+\gamma-p(n-\alpha)-(q+1)(n-\beta)=0$,
$n+\beta+\tau-(p+1)(n-\alpha)-q(n-\beta)=0$,
we show that the positive solution of above system are radical symmetric
and decreasing about some point by using the method of
$Moving\ planes$ in
$\mathbb{R}^{n}$.
Population pharmacokinetic model to generate mechanistic insights in bile acid homeostasis and drug-induced cholestasis
Authors: Bruijn Véronique de , Rietjens Ivonne , Bouwmeester Hans
Published: January 2024

Background and Purpose – Bile acids (BA) fulfill a wide range of
physiological functions, but are also involved in pathologies, such as
cholestasis. Cholestasis is characterized by an intrahepatic
accumulation of BAs and subsequent spillage to the systemic circulation.
The aim of the present study was to develop a physiologically based
pharmacokinetic (PBPK) model, that would provide a tool to predict
dose-dependent BA accumulation in humans upon treatment with a Bile Salt
Export Pump (BSEP) inhibitor. Experimental Approach – We developed a
PBPK model describing the bile acid homeostasis using
glycochenodeoxycholic acid as an exemplary BA. We compared
interindividual differences by scaling the total amount of bile acids
with an empirical scaling factor and alternatively by sampling randomly
from a distribution describing the BSEP abundances using Markov Chain
Monte Carlo simulations. Next, the effects of the BSEP-inhibitor
bosentan on the bile acid levels were simulated. Key Results – The
developed model adequately predicted the in vivo BA dynamics. An
empirical scaling factor of 0.5 or 1.5 could readily describe the
variations within and between data in human volunteers. Bosentan
treatment disproportionally increased the maximum BA concentration in
individuals with a large total BA pool or low BSEP abundance. Conclusion
and Implications - Especially a large total BA pool size and a low BSEP
abundance were predicted to be risk factors for rapid saturation of BSEP
and subsequent intrahepatic BA accumulation. This model provides a first
estimate of safe therapeutic external dose levels of compounds with
BSEP-inhibitory properties.
Graphene spin coated electrode for polyacrylonitrile acoustic nanogenerators
Authors: Aghayari Sedigheh
Published: January 2024

In the recent years piezoelectric nanogenerators due to their more
durability in high dust or humidity are mor attractive than
triboelectric ones. So, increasing the outputs of them is the subject of
many researches. I focused on electrodes of the acoustic nanofibers
nanogenerators for the first time. Compare to other works here I want to
introduce a new electrode which is cheaper and do not result in lower
outputs. Here for the first time, graphene spin coated ink used for
polyacrylonitrile based acoustic nanogenerator. The results of the
acoustic tests compared with in-situ synthesis of nickel nanoparticles
on the layer and using graphene spin coated screen ink and using
conductive tapes. Finally, producing sound by this graphene ink was done
too.
Cancer does not wait: safeguarding care for pediatric acute lymphoblastic leukemia patients during the COVID-19 pandemic in a Mexican hospital.
Authors: Colunga-Pedraza Julia , Colunga-Pedraza Perla , Arrieta Jafet , Neira Rodrigo Ortiz , Jiménez-Antolinez Valentine , Lopez Hilda Benavides , Lozano Samantha Peña , Ramírez-Cortinas Sergio , Bejarano Jose Castillo , Villar Alejandra Pérez , González-Llano Oscar
Published: January 2024

Introduction: Safeguarding care of acute lymphoblastic leukemia (ALL)
patients during the COVID-19 pandemic has posed a significant challenge
to health systems. We documented continuity (timeliness and compliance)
of curative treatment in pediatric ALL patients, and the incidence and
outcomes of patients infected with SARS-Cov2 in our institution.
Materials and methods: We included all ALL patients aged <16
years who received treatment in “Hospital Universitario Dr. José
Eleuterio Gonzalez” from March 2020 to June 2021. The causes of
non-compliance and the outcomes of patients with COVID-19 were reported.
Results: We analyzed 553 visits from 89 patients with ALL. Chemotherapy
administration was timely and compliant with our treatment protocol in
83% (n=459) of the records reviewed. Treatment continuity goals of
≥80% were achieved for all treatment phases, except for the
consolidation (66%) and intermediate maintenance phases (60%). The
main causes of treatment delays or treatment modifications included lack
of financial resources (2.5%), lack of inpatient beds (1.8%) and
chemotherapy stock-outs (1.3%), and the treatment abandonment rate was
3.3%. Twenty-two patients (24.7%) were diagnosed with COVID-19. Of
these, seven (32%) developed pneumonia, five (22.7%) required oxygen,
and two (9%) developed multisystem inflammatory syndrome. For patients
with COVID-19, the median length of stay was 9.5 days, and the 30-day
mortality rate was 4.5%. Discussion: The continuity of curative
treatment for ALL in the context of COVID-19 was >80% in
our hospital. Adapting diagnosis and treatment protocols and
implementing strategies to minimize the risk of infection were
fundamental to safeguarding continuity care for cancer patients.
Health workers' perception and emotions of teleconsultation in pediatric cancer care
Authors: Calò Pierluigi , Bertrand Amandine , Girodet Magali , Gauchet Alice , Anota Amélie , Christophe Véronique , Leblond Pierre
Published: January 2024

Background and aims: During the first lockdown of the novel Coronavirus
pandemic (COVID-19), we developed a teleconsultation model to replace
in-person visits. We conducted a survey-based study with the aim to
evaluate satisfaction and emotions of health workers (HWs), to assess
the feasibility of teleconsultations and to evaluate technical issues.
Methods: This is a prospective monocentric exploratory study in which
data were collected from March until May 2020 in the Lyon Pediatric
Hematology and Oncology institute (IHOPe). We realized synchronous,
video-based consultations between HWs and pediatric patients, treated
for blood or solid malignancies or blood benign diseases. Every HW
completed an online, pseudo-anonymized questionnaire which covered
technical aspects, data concerning satisfaction, perception of the
quality of the interaction with the patient and emotions felt after the
teleconsultation. A score was calculated for each aspect. In order to
study the relationship between the scores, the correlation coefficient
method was used. Results: Eleven specialists participated in the study
and we selected 84 questionnaires. With a satisfaction rate of 74%, HWs
felt mostly calm (80%), relaxed (70%), stress-free (69%) and relieved
(65%). We calculated the following median scores: an overall
satisfaction score of 6.67 (0-10), a global feeling score of 8.79
(3.33-10.00), and a quality score of 7.34 (2.50-10.00). A strong
correlation between the quality of the teleconsultation and the
satisfaction of the HWs has been highlighted (r = 0,588). Conclusion:
Our series is an encouragingly positive experience from the perspective
of the HWs, their feelings and perceptions.