bluuebunny/crossref_metadata_2025 huggingface dataset:
Ice type matters: impacts of landfast and drift ice on body condition in a high Arctic seabird community
Authors: Sauser Christophe , Blévin Pierre , Chastel Olivier , Gabrielsen Geir Wing , Hanssen Sveinn Are , Lorentzen Erlend , Moe Børge , Moreau Sebastien , Sagerup Kjetil , Descamps Sebastien
Published: February 2024

Sea ice, a central component of polar ecosystems, is undergoing profound changes due to climate change. In particular, the Arctic is experiencing unprecedented warming at quicker rates than other regions. This alarming trend of sea ice loss has dire consequences, with spill-over effects on the entire ecosystem, from phytoplankton to top predators. The complex and dynamic nature of sea ice gives rise to diverse habitats, each with the potential to affect larger ecosystems in different ways. However, our understanding of the relative importance of different ice types for higher trophic levels remains limited. To address this knowledge gap, we conducted a comprehensive study of the effects of drift ice, landfast ice, and total sea ice extent (landfast ice + drift ice) on the body condition of six species of polar-breeding seabirds using long-term monitoring data (2003-2021) from Kongsfjorden, Svalbard. These species fell into two categories: Arctic species (Little Auk Alle alle, Brünnich’s Guillemot Uria lomvia, and Glaucous Gull Larus hyperboreus) and “boreal” (or north temperate) species (Black-legged Kittiwake Rissa tridactyla, Arctic Skua Stercorarius parasiticus, and Great Skua Stercorarius skua). We found that the presence and extent of different types of sea ice may have different effects on seabird body condition. Though we did not find any relationship between total sea-ice extent and seabird body condition, drift ice and landfast ice extent did produce significant effects. For Arctic species, these effects were positive. For boreal species, the relationship between body condition and drift and landfast ice was more complex. Our study suggests that the use of a non-specific sea ice variable may mask the effects of sea ice on Arctic wildlife, highlighting the importance of not considering sea ice to be uniform and simple habitat.
The value of lung ultrasound score in neonatal respiratory distress syndrome: a prospective diagnostic cohort study
Authors: Huang Chunyan , Zhang Shaoqin , Ha Xiaoming , Cui Yanfang , Zhang Hongxia
Published: February 2024

Rationale

The accurate diagnosis of critically ill patients with respiratory failure can be achieved through lung ultrasound (LUS) score. Considering its characteristics, it is speculated that this technique might also be useful for patients with neonatal respiratory distress syndrome (NRDS). Thus, there is a need for precise imaging tools to monitor such patients.

Objectives

This double-blind randomized cohort study aims to investigate the impact of LUS and related scores on the severity of NRDS patients.

Methods

This study was conducted as a prospective double-blind randomized study. Bivariate correlation analysis was conducted to investigate the relationship between LUS score and Oxygenation Index (OI), Respiratory Index (RI), and Sequential Organ Failure Assessment (SOFA) score. Spearman’s correlation coefficient was used to generate correlation heat maps, elucidating the associations between LUS and respective parameters in different cohorts. Receiver Operating Characteristic (ROC) curves were employed to calculate the predictive values, sensitivity, and specificity of different scores in determining the severity of NRDS.

Results

This study ultimately included 134 patients admitted to the intensive care unit (ICU) between December 2020 and June 2022. Among these patients, 72 were included in the NRDS cohort, while 62 were included in the Non-NRDS (N-NRDS) cohort. There were significant differences in the mean LUS scores between NRDS and N-NRDS patients (p < 0.01). The LUS score was significantly negatively correlated with the OI (p < 0.01), while it was significantly positively correlated with the RI and SOFA scores (p < 0.01). The correlation heatmap revealed the highest positive correlation coefficient between LUS and RI (0.82), while the highest negative correlation coefficient was observed between LUS and OI (−0.8). ROC curves for different scores demonstrated that LUS score had the highest area under the curve (0.91, 95% CI: 0.84–0.98) in predicting the severity of patients’ conditions. The combination of LUS and other scores can more accurately predict the severity of NRDS patients, with the highest AUC value of 0.93, significantly higher than using a single indicator alone (p < 0.01).

Conclusion

Our double-blind randomized cohort study demonstrates that LUS, RI, OI, and SOFA scores can effectively monitor the lung ventilation and function in NRDS. Moreover, these parameters and their combination have significant predictive value in evaluating the severity and prognosis of NRDS patients. Therefore, these results provide crucial insights for future research endeavors.
PRRSV-2 viral load in critical non-lymphoid tissues is associated with late gestation fetal compromise
Authors: Rudy K. , Jeon D. , Smith A. A. , Harding J. C. S. , Pasternak J. A.
Published: February 2024

The impact of late gestation PRRSV-2 infection is highly variable within a litter, with a subset of fetuses displaying varying degrees of compromise following infection while others remain viable despite significant systemic viral load. To understand the underlying cause of this variation, we examined the susceptibility, distribution and impact of viral infection within non-lymphoid tissues. Samples of brain, heart, kidney, liver, lung, and skeletal muscle were obtained from fetuses of pregnant gilts at gestation day 86, and the presence and distribution of CD163+ cells within each tissue evaluated via immunohistofluorescence. Equivalent samples were collected from phenotypic extremes representing resistant, resilient and susceptible fetuses at 21 days following infection of pregnant gilts with PRRSV-2 at day 86 of gestation. Viral load and its impact in each tissue was evaluated by a combination of qPCR, in vitro viral recovery, and local expression of IFNG and CD163. Resting populations of CD163+ cells were observed in all six non-lymphoid tissues from healthy day 86 fetuses, though the apparent density and the morphology of positive cells varied between tissue. Viral RNA was detected in all six tissues derived from fetuses previously classified as highly infected, and infectious viral particles successfully recovered. Significantly more viral RNA was detected in heart, brain, lung and skeletal muscle of susceptible fetuses, relative to their viable counterparts. Infection was associated with an increase in the expression of CD163 in brain, kidney and lung. In addition, the presence of virus in each tissue coincided with a significant upregulation in the expression of IFNG, but the scale of this response was not associated with fetal susceptibility. Thus, PRRSV-2 is widely distributed across these susceptible non-lymphoid fetal tissues, and fetal outcome is associated with local viral load in critical fetal organs.
ATH-1105, a small-molecule positive modulator of the neurotrophic HGF system, is neuroprotective, preserves neuromotor function, and extends survival in preclinical models of ALS
Authors: Berthiaume Andrée-Anne , Reda Sherif M. , Kleist Kayla N. , Setti Sharay E. , Wu Wei , Johnston Jewel L. , Taylor Robert W. , Stein Liana R. , Moebius Hans J. , Church Kevin J.
Published: February 2024

Introduction

Amyotrophic lateral sclerosis (ALS), a progressive and fatal neurodegenerative disorder, primarily affects the motor neurons of the brain and spinal cord. Like other neurodegenerative conditions, ongoing pathological processes such as increased inflammation, excitotoxicity, and protein accumulation contribute to neuronal death. Hepatocyte growth factor (HGF) signaling through the MET receptor promotes pro-survival, anti-apoptotic, and anti-inflammatory effects in multiple cell types, including the neurons and support cells of the nervous system. This pleiotropic system is therefore a potential therapeutic target for treatment of neurodegenerative disorders such as ALS. Here, we test the effects of ATH-1105, a small-molecule positive modulator of the HGF signaling system, in preclinical models of ALS.

Methods

In vitro, the impact of ATH-1105 on HGF-mediated signaling was assessed via phosphorylation assays for MET, extracellular signal–regulated kinase (ERK), and protein kinase B (AKT). Neuroprotective effects of ATH-1105 were evaluated in rat primary neuron models including spinal motor neurons, motor neuron-astrocyte cocultures, and motor neuron-human muscle cocultures. The anti-inflammatory effects of ATH-1105 were evaluated in microglia- and macrophage-like cell systems exposed to lipopolysaccharide (LPS). In vivo, the impact of daily oral treatment with ATH-1105 was evaluated in Prp-TDP43A315T hemizygous transgenic ALS mice.

Results

In vitro, ATH-1105 augmented phosphorylation of MET, ERK, and AKT. ATH-1105 attenuated glutamate-mediated excitotoxicity in primary motor neurons and motor neuron- astrocyte cocultures, and had protective effects on motor neurons and neuromuscular junctions in motor neuron-muscle cocultures. ATH-1105 mitigated LPS-induced inflammation in microglia- and macrophage-like cell systems. In vivo, ATH-1105 treatment resulted in improved motor and nerve function, sciatic nerve axon and myelin integrity, and survival in ALS mice. Treatment with ATH-1105 also led to reductions in levels of plasma biomarkers of inflammation and neurodegeneration, along with decreased pathological protein accumulation (phospho-TDP-43) in the sciatic nerve. Additionally, both early intervention (treatment initiation at 1 month of age) and delayed intervention (treatment initiation at 2 months of age) with ATH-1105 produced benefits in this preclinical model of ALS.

Discussion

The consistent neuroprotective and anti-inflammatory effects demonstrated by ATH-1105 preclinically provide a compelling rationale for therapeutic interventions that leverage the positive modulation of the HGF pathway as a treatment for ALS.
From theory to practice: challenges and rewards of implementing ketogenic metabolic therapy in mental health
Authors: Laurent Nicole
Published: February 2024

This perspective article delves into the implementation of Ketogenic Metabolic Therapy (KMT) by a mental health counselor who attempts to bridge the gap between emerging research and real-world clinical application. Grounded in the author’s clinical experiences, the article communicates the potential of KMT in mental health care, highlighting both its therapeutic promise and the insights gained from hands-on patient interactions. While the adoption of KMT necessitates adjustments in societal, emotional, and dietary domains, especially within diverse mental health contexts, these challenges are surmountable with appropriate guidance and support. The article encourages the capture of qualitative data alongside quantitative measures and advocates for an approach that considers the broader implications of improved mental well-being on families and communities. As the field advances, interdisciplinary collaborations between researchers and clinicians will be pivotal in refining and expanding the application of KMT, ultimately enhancing patient outcomes and elevating the standard of mental health care.
ERBB2 (HER2) amplifications and co-occurring KRAS alterations in the circulating cell-free DNA of pancreatic ductal adenocarcinoma patients and response to HER2 inhibition
Authors: Barzi Afsaneh , Weipert Caroline M. , Espenschied Carin R. , Raymond Victoria M. , Wang-Gillam Andrea , Nezami Mohammad Amin , Gordon Eva J. , Mahadevan Daruka , Mody Kabir
Published: February 2024

Purpose

Despite accumulating data regarding the genomic landscape of pancreatic ductal adenocarcinoma (PDAC), olaparib is the only biomarker-driven FDA-approved targeted therapy with a PDAC-specific approval. Treating ERBB2(HER2)-amplified PDAC with anti-HER2 therapy has been reported with mixed results. Most pancreatic adenocarcinomas have KRAS alterations, which have been shown to be a marker of resistance to HER2-targeted therapies in other malignancies, though the impact of these alterations in pancreatic cancer is unknown. We describe two cases of ERBB2-amplified pancreatic cancer patients treated with anti-HER2 therapy and provide data on the frequency of ERBB2 amplifications and KRAS alterations identified by clinical circulating cell-free DNA testing.

Methods

De-identified molecular test results for all patients with pancreatic cancer who received clinical cell-free circulating DNA analysis (Guardant360) between 06/2014 and 01/2018 were analyzed. Cell-free circulating DNA analysis included next-generation sequencing of up to 73 genes, including select small insertion/deletions, copy number amplifications, and fusions.

Results

Of 1,791 patients with pancreatic adenocarcinoma, 36 (2.0%) had an ERBB2 amplification, 26 (72.2%) of whom had a KRAS alteration. Treatment data were available for seven patients. Two were treated with anti-HER2 therapy after their cell-free circulating DNA result, with both benefiting from therapy, including one with a durable response to trastuzumab and no KRAS alteration detected until progression.

Conclusion

Our case series illustrates that certain patients with ERBB2-amplified pancreatic adenocarcinoma may respond to anti-HER2 therapy and gain several months of prolonged survival. Our data suggests KRAS mutations as a possible mechanism of primary and acquired resistance to anti-HER2 therapy in pancreatic cancer. Additional studies are needed to clarify the role of KRAS in resistance to anti-HER2 therapy.
Case Report: In situ pulmonary artery thrombosis in a 12-year-old girl classified as systemic lupus erythematosus
Authors: Feng Yong , Chen Ning , Dai Bing , Shang Yunxiao
Published: February 2024

In situ pulmonary artery thrombosis (ISPAT) is a relatively rare but potentially life-threatening complication of systemic lupus erythematosus (SLE) in children. We report the case of a 12-year-old girl who presented with fever, chest pain, and dyspnea. Immune thrombocytopenia was identified due to purpura and menorrhagia 3 months before presentation with a lowest platelet count of 12 × 109/L. The sudden onset of fever, chest pain, and dyspnea were misdiagnosed as hyperinflammatory responses caused by pneumonia; these symptoms ameliorated with glucocorticoid and antibiotic treatment. The reappearance of symptoms after dose reduction of glucocorticoids and the observation of bloody bronchoalveolar lavage fluid necessitated further evaluation. Pulmonary artery thrombosis/embolism was identified using computed tomography pulmonary angiography and high D-dimer quantitative level of 4,118 μg/L (normal <252 μg/L). Ultrasonography of the deep and superficial veins of both lower limbs and renal veins revealed no thrombosis, suggesting the diagnosis of ISPAT. Further etiological evaluation revealed positive antinuclear antibodies, lupus anticoagulant, and anti-SSA antibodies, confirming SLE. Repeated normal urine analysis indicated that lupus nephritis was unlikely. Further, the negative anticardiolipin and anti-β2 glycoprotein antibodies and temporary positive lupus anticoagulant suggested that antiphospholipid syndrome was unlikely. The patient received anticoagulants, glucocorticoids, hydroxychloroquine, and mycophenolate therapy. Her symptoms gradually improved, and she was discharged. At the 1-month follow-up, the thrombosis had resolved. During the 1-year follow-up, her condition remained well without SLE relapse. Our experience with this case emphasizes searching for SLE in the case of ISPAT and pulmonary hemorrhages. ISPAT can occur in children with SLE and may be caused by hyperinflammatory response during SLE flare.
Herb-drug interactions of silybinin and cilofexor in beagle dogs based on pharmacokinetics by UPLC-MS/MS
Authors: Wei Xinyi , Su Yanding , Cheng Qian , Liang Songmao , Zhang Tingping , Duan Lengxin , Shen Xiuwei , Qiu Xiangjun
Published: February 2024

Objective: A remarkably sensitive, accurate, and efficient ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach was developed as a facile and expeditious method for measuring cilofexor concentration in beagle dogs, the herb-drug interactions between silybinin and cilofexor was explored based on pharmacokinetics.

Methods: The plasma sample protein of the beagles were rapidly sedimented with acetonitrile, and cilofexor and tropifexor (internal standard, ISTD) were separated by gradient elution using a 0.1% formic acid aqueous solution and acetonitrile as the mobile phase. The concentrations were detected using positive ion multiple reaction monitoring (MRM) mode. Mass transfer pairs were m/z 587.91→267.91 for cilofexor and m/z 604.08→228.03 for ISTD, respectively. A two-period self-controlled experimental design was adopted for the HDIs experiment. In the first period (Group A), six beagle dogs were orally administered cilofexor at a dose of 1 mg/kg. In the second period (Group B), silybinin (3 mg/kg) was orally administered to the six beagle dogs twice a day for seven consecutive days, after which cilofexor was orally administered. The cilofexor concentration in beagle dogs was determined, and HDIs were evaluated based on their pharmacokinetics.

Results: The accuracy and precision of cilofexor were both less than 15%, and the recoveries, matrix effects, and stability met the relevant requirements. The Cmax of cilofexor in group B was 49.62% higher than that in group A, whereas the AUC(0-t) and AUC(0−∞) of cilofexor in group B were 47.85% and 48.52% higher, respectively, than those in group A. Meanwhile, the t1/2 extended from 7.84 h to 9.45 h, CL and Vz decreased in Group B.

Conclusion: A novel UPLC-MS/MS approach was successfully applied for the measurement of cilofexor in beagle dog plasma. Silybinin can alter the pharmacokinetics of cilofexor in beagle dogs, thereby increasing plasma exposure to cilofexor.
Research on intrusion and large arch bulge in lining structure for highway’s mudstone tunnel
Authors: Li Shuai , Fu Jing , Liu Gengren , Zhou Jinghu
Published: February 2024

During the construction of a highway in northwest China, large deformation of mudstone caused severe deformation of and damage to side walls, initial support, and secondary lining to various extents. To reveal the causes of mudstone’s large deformation in the tunnels of this highway, a comprehensive study was conducted by using engineering geological survey, on-site monitoring and measurement, indoor rock mechanics test, numerical simulation, and macroscopic analysis. For the problem of large deformation of this highway’s tunnel section from YK209 + 500 m to YK210 + 030 m, the 3D finite difference method FLAC3D was used to simulate the large deformation of the wall rock and compare the deformation of the tunnel and the mechanical characteristics of the lining structure under different conditions by means of inverse analysis of the rheological characteristics of the mudstone and simulation of the softening of the mudstone in water. The research results provide a reference and basis for the construction design of similar mudstone tunnel projects. For the management of tunnel deformation, it is recommended to enhance the tunnel’s drainage measures, thereby mitigating the intensification of mudstone softening when exposed to water.
Intercellular communication in peritoneal dialysis
Authors: Sheng Li , Shan Yun , Dai Huibo , Yu Manshu , Sun Jinyi , Huang Liyan , Wang Funing , Sheng Meixiao
Published: February 2024

Long-term peritoneal dialysis (PD) causes structural and functional alterations of the peritoneal membrane. Peritoneal deterioration and fibrosis are multicellular and multimolecular processes. Under stimulation by deleterious factors such as non-biocompatibility of PD solution, various cells in the abdominal cavity show differing characteristics, such as the secretion of different cytokines, varying protein expression levels, and transdifferentiation into other cells. In this review, we discuss the role of various cells in the abdominal cavity and their interactions in the pathogenesis of PD. An in-depth understanding of intercellular communication and inter-organ communication in PD will lead to a better understanding of the pathogenesis of this disease, enabling the development of novel therapeutic targets.
Microbiome structure variation and soybean’s defense responses during flooding stress and elevated CO2
Authors: Coffman Lauryn , Mejia Hector D. , Alicea Yelinska , Mustafa Raneem , Ahmad Waqar , Crawford Kerri , Khan Abdul Latif
Published: February 2024

Introduction

With current trends in global climate change, both flooding episodes and higher levels of CO2 have been key factors to impact plant growth and stress tolerance. Very little is known about how both factors can influence the microbiome diversity and function, especially in tolerant soybean cultivars. This work aims to (i) elucidate the impact of flooding stress and increased levels of CO2 on the plant defenses and (ii) understand the microbiome diversity during flooding stress and elevated CO2 (eCO2).

Methods

We used next-generation sequencing and bioinformatic methods to show the impact of natural flooding and eCO2 on the microbiome architecture of soybean plants' below- (soil) and above-ground organs (root and shoot). We used high throughput rhizospheric extra-cellular enzymes and molecular analysis of plant defense-related genes to understand microbial diversity in plant responses during eCO2 and flooding.

Results

Results revealed that bacterial and fungal diversity was substantially higher in combined flooding and eCO2 treatments than in non-flooding control. Microbial diversity was soil>root>shoot in response to flooding and eCO2. We found that sole treatment of eCO2 and flooding had significant abundances of Chitinophaga, Clostridium, and Bacillus. Whereas the combination of flooding and eCO2 conditions showed a significant abundance of Trichoderma and Gibberella. Rhizospheric extra-cellular enzyme activities were significantly higher in eCO2 than flooding or its combination with eCO2. Plant defense responses were significantly regulated by the oxidative stress enzyme activities and gene expression of Elongation factor 1 and Alcohol dehydrogenase 2 in floodings and eCO2 treatments in soybean plant root or shoot parts.

Conclusion

This work suggests that climatic-induced changes in eCO2 and submergence can reshape microbiome structure and host defenses, essential in plant breeding and developing stress-tolerant crops. This work can help in identifying core-microbiome species that are unique to flooding stress environments and increasing eCO2.
Exploring the non-linear relationship and synergistic effect between urban built environment and public sentiment integrating macro- and micro-level perspective: a case study in San Francisco
Authors: He Pingge , Yu Bingjie , Ma Jiexi , Luo Keqian , Chen Siting , Shen Zhongwei
Published: February 2024

Public sentiment can effectively evaluate the public’s feelings of well-being in the urban environment and reflect the quality of the spatial environment to a certain extent. Previous studies on the relationship between public sentiment and urban built environmental factors have yielded meaningful results. However, few studies have focused on the effect of micro-built environment on public sentiment at the street level, which directly shapes people’s perceptions. In addition, the nonlinear relationship and synergistic effect among urban built environmental factors have been commonly disregarded in previous studies, resulting in an incomplete understanding of the impact of urban built environment on public emotions. Therefore, this paper takes San Francisco as a study case to explore the complex relationship between urban built environmental factors and public emotions. Specifically, this paper measures the polarity of public emotions through sentiment analysis on Twitter data, establishes a comprehensive built environment index system from both macro- and micro- perspectives, and subsequently explores the complex relationship between the urban built environment and public sentiment through the OLS model and Shapley Additive Explanation algorithm. Results show that: (1) micro-built environmental factors have a significant influence on public emotion, although they have been frequently ignored. (2) Public sentiment tends to be more positive in areas with recreation facilities, mixed land use, rich street view visual environment, suitable thermal and acoustic environment, balanced income, and a suitable degree of high population density. (3) A nonlinear relationship and threshold effect exist between the built environmental variables and the semantic orientations of public emotion. Environment improvement strategies based on the synergic effect between variables can effectively promote the generation of positive emotions. Our empirical findings can offer valuable insights to promote feelings of well-being and foster an urban development approach through strategic interventions within the urban built environment.
Time reference in aphasia: are there differences between tenses and aphasia fluency type? A systematic review and individual participant data meta-analysis
Authors: Cordonier Natacha , Schaffner Evodie , Zeroual Lana , Fossard Marion
Published: February 2024

Time reference is used to build the temporal framework of discourse and is essential in ensuring efficient communication. Several studies have reported time reference deficits in fluent and non-fluent aphasia and have shown that tenses (past, present, future) are not all impaired to the same extent. However, there is little consensus on the dissociations between tenses, and the question of the influence of the type of aphasia (fluent vs. non-fluent) on time reference remains open. Therefore, a systematic review and an individual participant data meta-analysis (or mega-analysis) were conducted to determine (1) whether one tense is more impaired than another in fluent and non-fluent aphasia and, if so, (2) which task and speaker-related factors moderate tense effects. The systematic review resulted in 35 studies reporting the performance in time reference of 392 participants. The mega-analysis was then performed on 23 studies for a total of 232 participants and showed an alteration of past tense compared to present and future tenses in both types of aphasia. The analysis also showed a task and an age effect on time reference but no gender effect, independently of tenses. These results add to our knowledge of time reference in aphasia and have implications for future therapies.
Design and methodology for a proof of mechanism study of individualized neuronavigated continuous Theta burst stimulation for auditory processing in adolescents with autism spectrum disorder
Authors: Oberman Lindsay M. , Francis Sunday M. , Beynel Lysianne , Hynd Megan , Jaime Miguel , Robins Pei L. , Deng Zhi-De , Stout Jeff , van der Veen Jan Willem , Lisanby Sarah H.
Published: February 2024

It has been suggested that aberrant excitation/inhibition (E/I) balance and dysfunctional structure and function of relevant brain networks may underlie the symptoms of autism spectrum disorder (ASD). However, the nomological network linking these constructs to quantifiable measures and mechanistically relating these constructs to behavioral symptoms of ASD is lacking. Herein we describe a within-subject, controlled, proof-of-mechanism study investigating the pathophysiology of auditory/language processing in adolescents with ASD. We utilize neurophysiological and neuroimaging techniques including magnetic resonance spectroscopy (MRS), diffusion-weighted imaging (DWI), functional magnetic resonance imaging (fMRI), and magnetoencephalography (MEG) metrics of language network structure and function. Additionally, we apply a single, individually targeted session of continuous theta burst stimulation (cTBS) as an experimental probe of the impact of perturbation of the system on these neurophysiological and neuroimaging outcomes. MRS, fMRI, and MEG measures are evaluated at baseline and immediately prior to and following cTBS over the posterior superior temporal cortex (pSTC), a region involved in auditory and language processing deficits in ASD. Also, behavioral measures of ASD and language processing and DWI measures of auditory/language network structures are obtained at baseline to characterize the relationship between the neuroimaging and neurophysiological measures and baseline symptom presentation. We hypothesize that local gamma-aminobutyric acid (GABA) and glutamate concentrations (measured with MRS), and structural and functional activity and network connectivity (measured with DWI and fMRI), will significantly predict MEG indices of auditory/language processing and behavioral deficits in ASD. Furthermore, a single session of cTBS over left pSTC is hypothesized to lead to significant, acute changes in local glutamate and GABA concentration, functional activity and network connectivity, and MEG indices of auditory/language processing. We have completed the pilot phase of the study (n=20 Healthy Volunteer adults) and have begun enrollment for the main phase with adolescents with ASD (n=86; age 14-17). If successful, this study will establish a nomological network linking local E/I balance measures to functional and structural connectivity within relevant brain networks, ultimately connecting them to ASD symptoms. Furthermore, this study will inform future therapeutic trials using cTBS to treat the symptoms of ASD.
The prospective associations among time management tendency, negative emotions, and problematic smartphone use in Chinese nursing students: enlightenment from COVID-19
Authors: Ding Nani , Shi Jiaqi , Xu Huihui , Wang Xiaoyi , Liu Guilin , Mao Lijie , Zhang Guohua , Zhang Jingjing
Published: February 2024

Purpose

The regularity of epidemic prevention and control measures in China has meant that nursing students have been exposed to more electronic devices, while problematic smartphone use has increased. The purpose of this study is to determine the prospective associations among time management tendency, negative emotions, and problematic smartphone use in nursing students during the COVID-19 pandemic.

Methods

A longitudinal study was conducted between November 2021 and May 2022. A total of 989 nursing students participated. The convenience sampling method was adopted and the following tools were used: the Adolescence Time Management Disposition Scale, the Depression Anxiety Stress Scales – 21, and the Mobile Phone Addiction Index. Multiple parallel mediation models were used by Mplus.

Results

Time management tendency had a significantly negative effect on problematic smartphone use (p < 0.05). Further tests using mediation models showed that stress as a negative emotion mediated the relationship between time management tendency and problematic smartphone use (p < 0.05) over time.

Conclusion

Nursing educators need to strengthen the stress resistance and time management ability of nursing students.
Association between frailty and hepatic fibrosis in NAFLD among middle-aged and older adults: results from NHANES 2017–2020
Authors: Atabieke Falide , Li Xiu-Juan , Aierken Ailikamu , Li Jian , Zhang Yu , Aizezi Yierzhati , Gao Hong-Liang , Zhang Zhi-Qiang
Published: February 2024

Background

Although previous studies found that frailty is prevalent in NAFLD patients with advanced liver fibrosis and cirrhosis, studies examining the relationship are spare.

Aim

Our study aspires to investigate the potential correlation between the Frailty Index (FI) and hepatic fibrosis among middle-aged and older adults with NAFLD.

Methods

Data from the 2017–2020.03 National Health and Nutrition Examination Survey (NHANES) were utilized for this study, with a final of 2,383 participants aged 50 years and older included. The quantification of frailty was executed employing a 49-item frailty index. The recognition of hepatic steatosis and fibrosis was accomplished through the utilization of the controlling attenuation parameter (CAP) and transient elastography (TE). The relationship between the FI and hepatic fibrosis were investigated employing univariable and multivariable-adjusted logistic regression analyses. A subgroup analysis was conducted, dividing the subjects based on gender, Body Mass Index (BMI), and the presence of hyperlipidemia.

Results

The findings demonstrated a positive correlation between the FI and significant hepatic fibrosis in NAFLD, even after using multivariate logistic regression models adjusting for potential confounding factors (OR = 1.022, 95% CI, 1.004–1.041) and in tertiles (Q3vs Q1: OR = 2.004, 95% CI, 1.162–3.455). In the subgroup analysis, the correlation was more statistically significant in male (OR = 1.046, 95% CI, 1.022–1.071), under/normal weight (OR = 1.077, 95% CI, 1.009–1.150), overweight (OR = 1.040, 95% CI, 1.010–1.071), and subjects without hyperlipidemia (OR = 1.054, 95% CI, 1.012–1.097). The area under the Receiver Operating Characteristic (ROC) curve for the FI in assessing the existence of substantial fibrosis in NAFLD was 0.612 (95% CI, 0.596–0.628).

Conclusion

This study demonstrated a positive correlation between significant hepatic fibrosis and frailty, particularly among males aged 50 years and older, who were non-obese and did not have hyperlipidemia with NAFLD. Additional studies are required to further validate these findings.
Eating behavior during pregnancy mediates the association between depression and diet quality--a new strategy for intervention in pregnancy
Authors: Jin Xingyi , Zhu Jian , Wang Niannian , Sun Lingzhen , Yu Junhui , Wang Shaokang , Sun Guiju
Published: February 2024

Background

Depression can result in changes in eating behavior and decrease the quality of eating. It has been shown that maternal depression during pregnancy can result in malnutrition, which can have adverse effects on the pregnancy and the offspring. There is currently no clear association between depression and diet.

Methods

Five hundred and forty-nine pregnant women recruited from Danyang Maternal and Child Health Hospital in Jiangsu Province participated in this study and were administered the Intuitive Eating Scale-2 (IES-2), Edinburgh Post-natal Depression Scale (EPDS), Pregnancy Stress Scale (PPS), Self-rating Anxiety Scale (SAS), and Dietary Guidelines Adherence Index for Pregnant Women during Pregnancy (CDGCI-PW). The nutritional software collected dietary records for three consecutive days in mid-pregnancy to calculate dietary intake and nutrients that support energy production. The mediation analyses were conducted using SPSS 24.0 macro PROCESS.

Results

The relationship between depressive symptoms during pregnancy and diet quality was moderated primarily by two aspects of eating behavior, “Reliance on Hunger and Satiety Cues” (RHS) and “Body-Food Choice Congruence” (BFC). Depressive symptoms (EPDS scores) showed a negative correlation with RHS, BFC, and RHS, and BFC showed a positive correlation with diet quality, yielding a significant specific indirect effect. The multiple mediation model explained 14.7% of the variance in the diet quality.

Conclusion

This study highlights the important role of eating behaviors during pregnancy in the relationship between depressive symptoms (EPDS scores) and diet quality, and provides preliminary evidence for feasible ways pregnant women with depressive symptoms can improve diet quality, promote maternal and child health, and reduce depression.
A dynamic approach for visualizing and exploring concept hierarchies from textbooks
Authors: Wehnert Sabine , Chedella Praneeth , Asche Jonas , De Luca Ernesto William
Published: February 2024

In this study, we propose a visualization technique to explore and visualize concept hierarchies generated from a textbook in the legal domain. Through a human-centered design process, we developed a tool that allows users to effectively navigate through and explore complex hierarchical concepts in three kinds of traversal techniques: top-down, middle-out, and bottom-up. Our concept hierarchies offer an overview over a given domain, with increasing level of detail toward the bottom of the hierarchy which is consisting of entities. In the legal use case we considered, the concepts were adapted from section headings in a legal textbook, whereas references to law or legal cases inside the textbook became entities. The design of this tool is refined following various steps such as gathering user needs, pain points of an existing visualization, prototyping, testing, and refining. The resulting interface offers users several key features such as dynamic search and filter, explorable concept nodes, and a preview of leaf nodes at every stage. A high-fidelity prototype was created to test our theory and design. To test our concept, we used the System Usability Scale as a way to measure the prototype's usability, a task-based survey to asses the tool's ability in assisting users in gathering information and interacting with the prototype, and finally mouse tracking to understand user interaction patterns. Along with this, we gathered audio and video footage of users when participating in the study. This footage also helped us in getting feedback when the survey responses required further information. The data collected provided valuable insights to set the directions for extending this study. As a result, we have accounted for varying hierarchy depths, longer text spans than only one to two words in the elements of the hierarchy, searchability, and exploration of the hierarchies. At the same time, we aimed for minimizing visual clutter and cognitive overload. We show that existing approaches are not suitable to visualize the type of data which we support with our visualization.
Machine learning-based infant crying interpretation
Authors: Hammoud Mohammed , Getahun Melaku N. , Baldycheva Anna , Somov Andrey
Published: February 2024

Crying is an inevitable character trait that occurs throughout the growth of infants, under conditions where the caregiver may have difficulty interpreting the underlying cause of the cry. Crying can be treated as an audio signal that carries a message about the infant's state, such as discomfort, hunger, and sickness. The primary infant caregiver requires traditional ways of understanding these feelings. Failing to understand them correctly can cause severe problems. Several methods attempt to solve this problem; however, proper audio feature representation and classifiers are necessary for better results. This study uses time-, frequency-, and time-frequency-domain feature representations to gain in-depth information from the data. The time-domain features include zero-crossing rate (ZCR) and root mean square (RMS), the frequency-domain feature includes the Mel-spectrogram, and the time-frequency-domain feature includes Mel-frequency cepstral coefficients (MFCCs). Moreover, time-series imaging algorithms are applied to transform 20 MFCC features into images using different algorithms: Gramian angular difference fields, Gramian angular summation fields, Markov transition fields, recurrence plots, and RGB GAF. Then, these features are provided to different machine learning classifiers, such as decision tree, random forest, K nearest neighbors, and bagging. The use of MFCCs, ZCR, and RMS as features achieved high performance, outperforming state of the art (SOTA). Optimal parameters are found via the grid search method using 10-fold cross-validation. Our MFCC-based random forest (RF) classifier approach achieved an accuracy of 96.39%, outperforming SOTA, the scalogram-based shuffleNet classifier, which had an accuracy of 95.17%.
Immune aging: biological mechanisms, clinical symptoms, and management in lung transplant recipients
Authors: Kapse Bhavya , Budev Marie M. , Singer Jonathan P. , Greenland John R.
Published: February 2024

While chronologic age can be precisely defined, clinical manifestations of advanced age occur in different ways and at different rates across individuals. The observed phenotype of advanced age likely reflects a superposition of several biological aging mechanisms which have gained increasing attention as the world contends with an aging population. Even within the immune system, there are multiple age-associated biological mechanisms at play, including telomere dysfunction, epigenetic dysregulation, immune senescence programs, and mitochondrial dysfunction. These biological mechanisms have associated clinical syndromes, such as telomere dysfunction leading to short telomere syndrome (STS), and optimal patient management may require recognition of biologically based aging syndromes. Within the clinical context of lung transplantation, select immune aging mechanisms are particularly pronounced. Indeed, STS is increasingly recognized as an indication for lung transplantation. At the same time, common aging phenotypes may be evoked by the stress of transplantation because lung allografts face a potent immune response, necessitating higher levels of immune suppression and associated toxicities, relative to other solid organs. Age-associated conditions exacerbated by lung transplant include bone marrow suppression, herpes viral infections, liver cirrhosis, hypogammaglobulinemia, frailty, and cancer risk. This review aims to dissect the molecular mechanisms of immune aging and describe their clinical manifestations in the context of lung transplantation. While these mechanisms are more likely to manifest in the context of lung transplantation, this mechanism-based approach to clinical syndromes of immune aging has broad relevance to geriatric medicine.
Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis
Authors: Velasco Carlos , Ortega Javier , Ricón Jaime , Romero Beatriz , de Juan Lucía , Domínguez Lucas , Domínguez Mercedes , Moreno Inmaculada , Álvarez Julio , Bezos Javier
Published: February 2024

Caprine tuberculosis (TB) is a zoonotic disease caused by members of the Mycobacterium tuberculosis complex. TB eradication programs in goats are based on the single and comparative intradermal tuberculin tests (SITT and CITT, respectively). Antibody-based diagnostic techniques have emerged as potential diagnostic tools for TB. P22 ELISA has been previously evaluated using samples collected after the intradermal tuberculin tests to maximize the sensitivity, a phenomenon known as booster effect. However, there is no information available on whether the use of this diagnostic strategy could lead to a decrease of its specificity (Sp). The aim of the present study was to elucidate the interference effect of a recent CITT on the Sp of the P22 ELISA in serum and milk samples collected at different times after the CITT from a TB-free herd (n = 113). The number of reactors to P22 ELISA was significantly higher (p < 0.01) on serum samples collected 15 days post-CITT compared to day 0, showing a decrease in Sp from 99.1% (95% CI; 95.2–99.8%) to 88.5% (95% CI; 81.3–93.2%). The number of reactors and the quantitative values of P22 ELISA were significantly higher (p < 0.01) in serum samples compared to milk. No significant (p > 0.05) changes in the Sp of the P22 ELISA were observed throughout the different time samplings using milk No significant (p > 0.05) changes were observed on days 30 and 60 post-CITT. In conclusion, the booster effect strategy may significantly decrease the Sp of P22 ELISA in TB-free herds when serum samples are used but not when milk is tested.
Aerodynamic Shape Optimization of Subsonic/Supersonic Flows Integrating Variable-Fidelity Longitudinal Trim Analysis
Authors: Wu Yacong , Huang Jun , Ji Boqian , Song Lei
Published: February 2024

Most existing studies on aerodynamic shape optimization have not considered longitudinal trim under control surface deflection, typically achieving self-trim through a constraint of zero pitching moment or adjusting the optimized configuration for longitudinal trim. However, adjustments to the optimized configuration might introduce additional drag, reducing overall optimization benefits. In this paper, a novel approach of incorporating control surface deflection for longitudinal trim in aerodynamic optimization is proposed. Firstly, an aerodynamic computation program based on the high-order panel method was developed, introducing velocity perturbations on specific mesh surfaces to simulate actual control surface deflections. Subsequently, a comprehensive optimization framework was established, encompassing parametric modeling, aerodynamic computation, and variable-fidelity control surface deflection analysis. Finally, aerodynamic optimization analysis was conducted under both subsonic and supersonic conditions. Thirty-one design variables were selected with the trimmed lift-to-drag ratio in cruising condition as the objective function and the control surface deflection angle as the constraint. The results indicated an 8.52% increase in the trimmed lift-to-drag ratio compared to the baseline model under subsonic conditions and an 8.1% increase under supersonic conditions.
A330-300 Wake Encounter by ARJ21 Aircraft
Authors: Luo Haotian , Pan Weijun , Wang Yidi , Luo Yuming
Published: February 2024

Today, aviation has grown significantly in importance. However, the challenge of flight delays has become increasingly severe due to the need for safe separation between aircraft to mitigate wake turbulence effects. The primary emphasis of this investigation resides in elucidating the evolutionary attributes of wake vortices in homogeneous isotropy turbulence. The large eddy simulation (LES) method is used to scrutinize the dynamic evolution of wake vortices engendered by an A333 aircraft in the atmospheric milieu and assess its ramifications on the ARJ21 aircraft. The research endeavor commences by formulating an LES methodology for the evolution of aircraft wake vortices, integrating adaptive grid technology to reduce the necessary grid volume significantly. This approach enables the implementation of axial and non-axial grid adaptive refinement, leading to more accurate simulations of both axial and non-axial vortices. Numerical simulations are conducted using the LES approach to scrutinize three distinct rates of turbulence dissipation amidst the ambient atmospheric turbulence, and the results are juxtaposed with Lidar measurements (Wind3D 6000 LiDAR) of wake vortices acquired at Chengdu Shuangliu International Airport (CTU). Subsequently, the rolling moment of the following aircraft is calculated, and three-dimensional hazard zones are determined for the A333. It is found that during the approach phase, the wake turbulence separation minima for an ARJ21 (CAT-F) following an A333 (CAT-B) is 3.35 NM, which represents a reduction of approximately 33% compared to ICAO RECAT (Wake Turbulence Re-categorization). The findings validate the dependability of the fine-grained mesh used in the vortex core region, engendered through the adaptive grid method, which proficiently captures the Crow instability and the interconnected phenomena of vortices in the numerical examination of aircraft wake. The safety of wake encounters primarily depends on the magnitude of environmental turbulence and the development of structural instability in wake vortices.