bluuebunny/crossref_metadata_2025 huggingface dataset:
Improvement of Enzymatic Glucose Conversion from Chestnut Shells through Optimization of KOH Pretreatment
Authors: Lee Kang Hyun , Lee Soo Kweon , Lee Jeongho , Kim Seunghee , Park Chulhwan , Kim Seung Wook , Yoo Hah Young
Published: April 2021

Worldwide, about one-third of food produced for human consumption is wasted, which includes byproducts from food processing, with a significant portion of the waste still being landfilled. The aim of this study is to convert chestnut shells (CNSs) from food processing into a valuable resource through bioprocesses. Currently, one of the highest barriers to bioprocess commercialization is low conversion of sugar from biomass, and KOH pretreatment was suggested to improve enzymatic digestibility (ED) of CNS. KOH concentration of 3% (w/w) was determined as a suitable pretreatment solution by a fundamental experiment. The reaction factors including temperature, time and solid/liquid (S/L) ratio were optimized (77.1 g/L CNS loading at 75 °C for 2.8 h) by response surface methodology (RSM). In the statistical model, temperature and time showed a relatively significant effect on the glucan content (GC) and ED, but S/L ratio was not. GC and ED of the untreated CNS were 45.1% and 12.7%, respectively. On the other hand, GC and ED of pretreated CNS were 83.2% and 48.4%, respectively, and which were significantly improved by about 1.8-fold and 3.8-fold compared to the control group. The improved ED through the optimization is expected to contribute to increasing the value of byproducts generated in food processing.
Dissemination of Aerosol and Splatter in Clinical Environment during Cavity Preparation: An In Vitro Study
Authors: Ahmed Muhammad Adeel , Jouhar Rizwan
Published: April 2021

Dental health care workers around the world are in a constant state of fear and anxiety because they work in a constrained space of the dental practice. During routine dental procedures, they are exposed to aerosol and splatter. These airborne particles pose a great risk of transmitting contagious infections to health care workers and patients, especially in an era of social distancing due to COVID-19. The current study was conducted to evaluate contamination amount, duration, the distance of aerosol, and splatter produced after cavity preparation using a two-hole and four-hole handpiece. The study was performed on a dental manikin in a dental simulation laboratory at the College of Dentistry, King Faisal University Al Ahsa. The dental manikin was set to a reclined position to simulate the clinical operatory position of the patient for dental restorative procedures. Aerosol and splatter were collected on Grade 1 qualitative cotton cellulose filter paper. These were placed on adhesive tape extending from the headrest of the dental manikin in six different directions (2, 4, 6, 8, 10, and 12 o’clock) for up to 60 inches and on certain positions of the operator and assistant such as the chest, head, forearms, upper leg, and inside facemask. Class V cavity preparation was done by the principal investigator at a specific time of 3 min on tooth #11 using a two-hole high-speed handpiece, then on the next day, Class V cavity preparation was performed on tooth #21 by a four-hole handpiece. High volume suction was used throughout the cavity preparation. Immediately after cavity preparation, the first filter paper disc was replaced with new ones in all positions. The second set of filter papers was removed after 30 min. Transparent grids were used to count the contamination area on the filter paper disc. No statistically significant difference was found in the mean amount of aerosol and splatter produced by both handpieces, however, a statistically significant difference was found in an amount of aerosol and splatter produced at a 12, 24, and 36 inches distance immediately after cavity preparation and 30 min after cavity preparation, regardless of the type of handpiece used. It is advisable to refrain from removing the personal protective barriers immediately after the procedure within the vicinity of the dental practice. The use of other adjuncts such as high volume suction to reduce the spread of aerosol and splatter is also recommended.
QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices
Authors: Pantano Licia , La Scala Ladislao , Olibrio Francesco , Galluzzo Francesco Giuseppe , Bongiorno Carmelo , Buscemi Maria Drussilla , Macaluso Andrea , Vella Antonio
Published: April 2021

We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), fumonisin (FUMO; FB1, FB2, FB3), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method’s performance was assessed by two different proficiencies and tested with 100 real samples.
Behavioral Implementation and Compliance of Anti-Epidemic Policy in the COVID-19 Crisis
Authors: Fu Chengzhe , Liao Liao , Huang Weijun
Published: April 2021

Different countries have introduced different urgent policies to control the spread of the novel coronavirus. The compliance behavior of these anti-epidemic policies has always been an important concern to governments, and its effects need to be tested. In recent years, many scholars have paid attention to the mechanism and intervention of policy compliance behavior, which helps to explain the mechanism of anti-epidemic compliance behavior, and to improve the effectiveness of anti-epidemic policy. Therefore, considering the characters of youth groups in the context of the novel coronavirus, this study takes campus anti-epidemic compliance behavior as the research topic, based on 680 effective samples of college students in China, in order to examine the effectiveness of these policies using an investigation experiment. This study revealed that the ‘Nudge’ policy instrument was the most effective way to guide individuals’ behavior during the coronavirus outbreak, the ‘Sermon’ instrument was the least recognized, and the ‘Whip’ instrument (a traditional and classical policy instrument) had its normal effect on individuals’ behavior. Additionally, it found that high accessibility in policy implementation results in more significant policy behavior. By taking the effects of different policy behaviors into consideration, governments may produce better and more effective policy implementation and compliance during the anti-epidemic period.
Cross-Cultural Adaptation and Psychometric Properties of the Malay Version of the Communication Skills Attitude Scale (CSAS) among Medical Students in Malaysia
Authors: Mohamad-Isa Mohamad-Zikri , Mohamed-Yassin Mohamed-Syarif , Badlishah-Sham Siti Fatimah , Baharudin Noorhida , Ramli Anis Safura
Published: April 2021

Communication is one of the fundamental skills in the medical profession. The Communication Skills Attitude Scale (CSAS) is a widely used questionnaire to measure the attitudes of medical students toward learning communication skills. It has been adapted and translated into many languages. The objective of this study was to adapt and translate the CSAS into the Malay language and determine its psychometric properties in medical students. This is a cross-sectional study involving 218 first-year Universiti Teknologi MARA students. Content validation, cross-cultural adaptation, translation, and face validation of the 26-item CSAS were performed according to established guidelines. Principal component analysis with direct oblimin rotation was used to determine the underlying structure of the CSAS-Malay. The reliability was assessed using Cronbach’s α coefficient for internal consistency and using the intraclass correlation coefficient for the test–retest reliability. Although the contents of the CSAS-Malay and the original version were conceptually equivalent, item 11 was removed during the content validation stage due to a low item content validity index (I-CVI < 1.00). Two subscales were derived from the remaining 25 items, which were the Positive Attitude Scale and the Negative Attitude Scale. Items 1 and 15 were removed due to poor factor loadings. The total variance explained by the final two-factor solution with three items removed was 30.8%. Cronbach’s α coefficients for both the Positive and Negative Attitude Scales in the final questionnaire were 0.815 and 0.614, respectively. It also showed a good reproducibility with intraclass correlation coefficient (ICC) values of 0.725–0.950 for all the items. This study provided preliminary information about the psychometric properties of the CSAS-Malay. The final 23-item questionnaire had a good construct validity, an acceptable internal consistency, and at least a moderate test–retest reproducibility. It can be used to assess the attitudes of medical students toward learning communication skills. Future research to improve the generalizability of the questionnaire should include medical students from other universities with diverse backgrounds.
Comparison of Mean Platelet Counts in Preterm Infants with and without Retinopathy of Prematurity
Authors: Lim Zi Di , Pheng Edwin , Min Evelyn Tai Li , Van Rostenberghe Hans , Shatriah Ismail
Published: April 2021

Platelets are a primary source of pro- and anti-angiogenic cytokines. However, the evidence of their role in retinopathy of prematurity (ROP) is controversial. This retrospective study aimed to compare mean weekly platelet counts between infants with and without ROP over the first 6 weeks of life. A total of 93 infants matched by gestational age and birth weight were recruited (31 with ROP, 62 without ROP). Weekly mean platelet counts and other related risk factors were documented. The repeated measure analysis of variance (ANOVA) and the repeated measure analysis of covariance (ANCOVA) were used to compare mean platelet counts over time between the two groups, with and without adjusting for confounders. We found significant differences in the weekly mean platelet counts of infants with and without ROP over the first 6 weeks of life (p = 0.002). These differences disappeared after adjusting for covariates (p = 0.489). Lower mean platelet counts in ROP infants are not directly related to ROP, but rather to the presence of other risk factors for ROP, such as culture-proven sepsis, blood transfusion and bronchopulmonary dysplasia.
SARS-CoV-2 Seroprevalence among Healthcare Workers in General Hospitals and Clinics in Japan
Authors: Yoshihara Tatsuya , Ito Kazuya , Zaitsu Masayoshi , Chung Eunhee , Aoyagi Izumi , Kaji Yoshikazu , Tsuru Tomomi , Yonemura Takuma , Yamaguchi Koji , Nakayama Shinichi , Tanaka Yosuke , Yurino Nobuo , Koyanagi Hideki , Matsuki Shunji , Urae Ryuji , Irie Shin
Published: April 2021

Coronavirus disease 2019 (COVID-19) has become a serious public health problem worldwide. In general, healthcare workers are considered to be at higher risk of COVID-19 infection. However, the prevalence of COVID-19 among healthcare workers in Japan is not well characterized. In this study, we aimed to examine the seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies among 2160 healthcare workers in hospitals and clinics that are not designated to treat COVID-19 patients in Japan. The prevalence of SARS-CoV-2 immunoglobulin G was 1.2% in August and October 2020 (during and after the second wave of the pandemic in Japan), which is relatively higher than that in the general population in Japan (0.03–0.91%). Because of the higher risk of COVID-19 infection, healthcare workers should be the top priority for further social support and vaccination against SARS-CoV-2.
Gratitude at Work Prospectively Predicts Lower Workplace Materialism: A Three-Wave Longitudinal Study in Chile
Authors: Unanue Jesús , Oriol Xavier , Oyanedel Juan Carlos , Rubio Andrés , Unanue Wenceslao
Published: April 2021

Materialism at work refers to a higher importance attached to extrinsic (e.g., money, fame, image) versus intrinsic (self-development, affiliation, community participation) employees’ ‘aspirations’. Research from self-determination theory has consistently found that materialism at work is strongly detrimental for both employees and organizations. For example, materialism is negatively associated with lower job satisfaction and engagement and positively associated with higher turnover intentions and job insecurity. Unfortunately, there are no viable strategies for reducing materialism in the workplace yet. In this sense, based on emergent research in psychology, we theorized that dispositional gratitude—a key construct within the Positive Organizational Psychology field—could be a protecting factor against materialism. Further, we conducted a three-wave longitudinal design among a large sample of Chilean workers (n = 1841) to test, for the first time, the longitudinal link between gratitude and materialism. We used two novel methodologies: A cross-lagged panel model (CLPM) to test between-person changes and a trait-state-occasion model (TSO) to test within-person changes. We found that both the CLPM as well as the TSO models showed that gratitude at work prospectively predicted further lower workplace materialism. Specifically, the CLPM shows that individuals with higher than average gratitude at Ti, are more likely to show lower than average materialism at Ti+1. The TSO shows that individuals with a higher than their usual level of gratitude at Ti are more likely to show a lower than their usual level of materialism at Ti+1. Important implications for materialism research as well as for the Positive Organizational Psychology field are discussed.
The Financial Burden of Cancer on Families in the United States
Authors: Richard Patrick , Patel Nilam , Lu Yuan-Chiao , Walker Regine , Younis Mustafa
Published: April 2021

This study examined the relationship between a diagnosis of cancer and the likelihood of having any out-of-pocket costs (OOPC) and medical debt, and the amounts of OOPC and medical debt, at the household level. We used the 2013 Panel Study of Income Dynamics, a continuous, representative panel survey that collects demographic, economic, and social data in the United States. The analytic sample included head of households and their spouse (if married), 18–64 years old. Two-part models were used. The first part consisted of logistic regression models and the second part consisted of generalized linear models with logarithmic link and a gamma distribution. Logistic regression results showed odds of 2.13 (CI: 1.27, 3.57, p < 0.01) for any OOPC and odds of 1.55 (CI: 0.93, 2.58, p < 0.1) for any medical debt for households in which either the head or spouse (if married) reported a diagnosis of cancer compared to those that did not report a diagnosis of cancer. Likewise, results from the second part of the model for households with a positive amount of OOPC showed an exponentiated coefficient of 1.73 (CI: 1.33, 2.25, p < 0.01) for households in which either the head or spouse (if married) reported a diagnosis of cancer compared to households without a diagnosis of cancer. This study shows that a diagnosis of cancer places a financial burden on families, particularly with all types of debt, in the United States even after controlling for differences between households with a diagnosis of cancer and those without a diagnosis of cancer.
Perfluoroalkyl Chemicals and Male Reproductive Health: Do PFOA and PFOS Increase Risk for Male Infertility?
Authors: Tarapore Pheruza , Ouyang Bin
Published: April 2021

Poly- and perfluoroalkyl substances (PFAS) are manmade synthetic chemicals which have been in existence for over 70 years. Though they are currently being phased out, their persistence in the environment is widespread. There is increasing evidence linking PFAS exposure to health effects, an issue of concern since PFAS such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) bioaccumulate in humans, with a half-life of years. Many epidemiological studies suggest that, worldwide, semen quality has decreased over the past several decades. One of the most worrying effects of PFOS and PFOA is their associations with lower testosterone levels, similar to clinical observations in infertile men. This review thus focuses on PFOS/PFOA-associated effects on male reproductive health. The sources of PFAS in drinking water are listed. The current epidemiological studies linking increased exposure to PFAS with lowered testosterone and semen quality, and evidence from rodent studies supporting their function as endocrine disruptors on the reproductive system, exhibiting non-monotonic dose responses, are noted. Finally, their mechanisms of action and possible toxic effects on the Leydig, Sertoli, and germ cells are discussed. Future research efforts must consider utilizing better human model systems for exposure, using more accurate PFAS exposure susceptibility windows, and improvements in statistical modeling of data to account for the endocrine disruptor properties of PFAS.
Hereditary Prostate Cancer: Genes Related, Target Therapy and Prevention
Authors: Vietri Maria Teresa , D’Elia Giovanna , Caliendo Gemma , Resse Marianna , Casamassimi Amelia , Passariello Luana , Albanese Luisa , Cioffi Michele , Molinari Anna Maria
Published: April 2021

Prostate cancer (PCa) is globally the second most diagnosed cancer type and the most common cause of cancer-related deaths in men. Family history of PCa, hereditary breast and ovarian cancer (HBOC) and Lynch syndromes (LS), are among the most important risk factors compared to age, race, ethnicity and environmental factors for PCa development. Hereditary prostate cancer (HPCa) has the highest heritability of any major cancer in men. The proportion of PCa attributable to hereditary factors has been estimated in the range of 5–15%. To date, the genes more consistently associated to HPCa susceptibility include mismatch repair (MMR) genes (MLH1, MSH2, MSH6, and PMS2) and homologous recombination genes (BRCA1/2, ATM, PALB2, CHEK2). Additional genes are also recommended to be integrated into specific research, including HOXB13, BRP1 and NSB1. Importantly, BRCA1/BRCA2 and ATM mutated patients potentially benefit from Poly (ADP-ribose) polymerase PARP inhibitors, through a mechanism of synthetic lethality, causing selective tumor cell cytotoxicity in cell lines. Moreover, the detection of germline alterations in MMR genes has therapeutic implications, as it may help to predict immunotherapy benefits. Here, we discuss the current knowledge of the genetic basis for inherited predisposition to PCa, the potential target therapy, and the role of active surveillance as a management strategy for patients with low-risk PCa. Finally, the current PCa guideline recommendations are reviewed.
Molecular and Biochemical Basis of Minocycline-Induced Hyperpigmentation—The Study on Normal Human Melanocytes Exposed to UVA and UVB Radiation
Authors: Rok Jakub , Rzepka Zuzanna , Kowalska Justyna , Banach Klaudia , Beberok Artur , Wrześniok Dorota
Published: April 2021

Minocycline is a drug which induces skin hyperpigmentation. Its frequency reaches up to 50% of treated patients. The adverse effect diminishes the great therapeutic potential of minocycline, including antibacterial, neuroprotective, anti-inflammatory and anti-cancer actions. It is supposed that an elevated melanin level and drug accumulation in melanin-containing cells are related to skin hyperpigmentation. This study aimed to evaluate molecular and biochemical mechanism of minocycline-induced hyperpigmentation in human normal melanocytes, as well as the contribution of UV radiation to this side effect. The experiments involved the evaluation of cyto- and phototoxic potential of the drug using cell imaging with light and confocal microscopes as well as biochemical and molecular analysis of melanogenesis. We showed that minocycline induced melanin synthesis in epidermal melanocytes. The action was intensified by UV irradiation, especially with the UVB spectrum. Minocycline stimulated the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase (TYR) gene. Higher levels of melanin and increased activity of tyrosinase were also observed in treated cells. Moreover, minocycline triggered the supranuclear accumulation of tyrosinase, similar to UV radiation. The decreased level of premelanosome protein PMEL17 observed in all minocycline-treated cultures suggests disorder of the formation, maturation or distribution of melanosomes. The study revealed that minocycline itself was able to enhance melanin synthesis. The action was intensified by irradiation, especially with the UVB spectrum. Demonstrated results confirmed the potential role of melanin and UV radiation minocycline-induced skin hyperpigmentation.
Human Somatostatin SST4 Receptor Transgenic Mice: Construction and Brain Expression Pattern Characterization
Authors: Nemes Balázs , Bölcskei Kata , Kecskés Angéla , Kormos Viktória , Gaszner Balázs , Aczél Timea , Hegedüs Dániel , Pintér Erika , Helyes Zsuzsanna , Sándor Zoltán
Published: April 2021

Somatostatin receptor subtype 4 (SST4) has been shown to mediate analgesic, antidepressant and anti-inflammatory functions without endocrine actions; therefore, it is proposed to be a novel target for drug development. To overcome the species differences of SST4 receptor expression and function between humans and mice, we generated an SST4 humanized mouse line to serve as a translational animal model for preclinical research. A transposon vector containing the hSSTR4 and reporter gene construct driven by the hSSTR4 regulatory elements were created. The vector was randomly inserted in Sstr4-deficient mice. hSSTR4 expression was detected by bioluminescent in vivo imaging of the luciferase reporter predominantly in the brain. RT-qPCR confirmed the expression of the human gene in the brain and various peripheral tissues consistent with the in vivo imaging. RNAscope in situ hybridization revealed the presence of hSSTR4 transcripts in glutamatergic excitatory neurons in the CA1 and CA2 regions of the hippocampus; in the GABAergic interneurons in the granular layer of the olfactory bulb and in both types of neurons in the primary somatosensory cortex, piriform cortex, prelimbic cortex and amygdala. This novel SST4 humanized mouse line might enable us to investigate the differences of human and mouse SST4 receptor expression and function and assess the effects of SST4 receptor agonist drug candidates.
Nitrogen-Doped Titanium Dioxide Mixed with Calcium Peroxide and Methylcellulose for Dental Bleaching under Visible Light Activation
Authors: Thacker Minal , Chen Yi-Ning , Lin Chun-Pin , Lin Feng-Huei
Published: April 2021

The available tooth whitening products in the market contain high concentrations of hydrogen peroxide (H2O2) as an active ingredient. Therefore, in order to curb the high H2O2 concentration and instability of liquid H2O2, this study evaluated the efficacy and cytotoxicity of the bleaching gel composed of 10% calcium peroxide (CaO2) and visible-light-activating nitrogen-doped titanium dioxide (N-TiO2) with methyl cellulose as a thickener. Extracted bovine teeth were discolored using coffee and black tea stain solution and were divided into two groups (n = 6). Bleaching was performed thrice on each tooth specimen in both the groups, with one minute of visible light irradiation during each bleaching time. The CIELAB L*a*b* values were measured pre- and post-bleaching. The N-TiO2 calcinated at 350 °C demonstrated a shift towards the visible light region by narrowing the band gap energy from 3.23 eV to 2.85 eV. The brightness (ΔL) and color difference (ΔE) increased as bleaching progressed each time in both the groups. ANOVA results showed that the number of bleaching significantly affected ΔE (p < 0.05). The formulated bleaching gel exhibits good biocompatibility and non-toxicity upon exposure to 3T3 cells. Our findings showed that CaO2-based bleaching gel at neutral pH could be a stable, safe, and effective substitute for tooth whitening products currently available in the market.
Relationship between Orexigenic Peptide Ghrelin Signal, Gender Difference and Disease
Authors: Yamada Chihiro
Published: April 2021

Growth hormone secretagogue receptor 1a (GHS-R1a), which is one of the G protein-coupled receptors (GPCRs), is involved in various physiological actions such as energy consumption, growth hormone secretion promoting action, and cardiovascular protective action. The ligand was searched for as an orphan receptor for a while, but the ligand was found to be acylated ghrelin (ghrelin) discovered by Kangawa and Kojima et al. in 1999. Recently, it has also been reported that dysregulation of GHS-R1a mediates reduced feeding in various diseases. On the other hand, since the physiological effects of ghrelin have been studied exclusively in male mice, few studies have been conducted on gender differences in ghrelin reactivity. In this review, we describe (1) the characteristics of GHS-R1a, (2) the role of ghrelin in hypophagia due to stress or anticancer drugs, and (3) the gender differences in the physiological effects of GHS-R1a and the influence of stress on it.
Macrophage-Derived Adenosine Deaminase 2 Correlates with M2 Macrophage Phenotype in Triple Negative Breast Cancer
Authors: Kutryb-Zajac Barbara , Harasim Gabriela , Jedrzejewska Agata , Krol Oliwia , Braczko Alicja , Jablonska Patrycja , Mierzejewska Paulina , Zielinski Jacek , Slominska Ewa M. , Smolenski Ryszard. T.
Published: April 2021

Several lines of evidence suggest that altered adenosine deaminase (ADA) activity, especially its ADA2 iso-enzyme, is associated with malignant breast cancer (BC) development. Triple-negative breast cancer (TNBC) is currently the most challenging BC subtype due to its metastatic potential and recurrence. Herein, we analyzed the sources of ADA iso-enzymes in TNBC by investigating the effects of cell-to-cell interactions between TNBC cells, macrophages, lymphocytes, and endothelial cells. We also examined the potential relationship between ADA activity and cancer progression in TNBC patients. In vitro analyses demonstrated that the interactions of immune and endothelial cells with MDA-MB-231 triple negative BC cells modulated their extracellular adenosine metabolism pattern. However, they caused an increase in the ADA1 activity, and did not alter ADA2 activity in cancer cells. In turn, the co-culture of MDA-MB-231 cells with THP-1 monocyte/macrophages, Jurkat cells, and human lung microvascular endothelial cells (HULEC) caused the increase in ADA2 activity on THP-1 cells and ADA1 activity on Jurkat cells and HULEC. Clinical sample analysis revealed that TNBC patients had higher plasma ADA2 activities and lower ADA1/ADA2 ratio at advanced stages of cancer development than in the initial stages, while patients with hormone receptor positive, HER2 negative (HR+HER2-), and triple positive (HR+HER2+) breast cancers at the same stages showed opposite trends. TNBC patients also demonstrated positive associations between plasma ADA2 activity and pro-tumor M2 macrophage markers, as well as between ADA1 activity and endothelial dysfunction or inflammatory parameters. The analysis of TNBC patients, at 6 and 12 months following cancer treatment, did not showed significant changes in plasma ADA activities and macrophage polarization markers, which may be the cause of their therapeutic failure. We conclude that alterations in both ADA iso-enzymes can play a role in breast cancer development and progression by the modulation of extracellular adenosine-dependent pathways. Additionally, the changes in ADA2 activity that may contribute to the differentiation of macrophages into unfavorable pro-tumor M2 phenotype deserve special attention in TNBC.
Pax3 and Pax7 Exhibit Distinct and Overlapping Functions in Marking Muscle Satellite Cells and Muscle Repair in a Marine Teleost, Sebastes schlegelii
Authors: Wang Mengya , Song Weihao , Jin Chaofan , Huang Kejia , Yu Qianwen , Qi Jie , Zhang Quanqi , He Yan
Published: April 2021

Pax3 and Pax7 are members of the Pax gene family which are essential for embryo and organ development. Both genes have been proved to be markers of muscle satellite cells and play key roles in the process of muscle growth and repair. Here, we identified two Pax3 genes (SsPax3a and SsPax3b) and two Pax7 genes (SsPax7a and SsPax7b) in a marine teleost, black rockfish (Sebastes schlegelii). Our results showed SsPax3 and SsPax7 marked distinct populations of muscle satellite cells, which originated from the multi-cell stage and somite stage, respectively. In addition, we constructed a muscle injury model to explore the function of these four genes during muscle repair. Hematoxylin–eosin (H–E) of injured muscle sections showed new-formed myofibers occurred at 16 days post-injury (dpi). ISH (in situ hybridization) analysis demonstrated that the expression level of SsPax3a and two SsPax7 genes increased gradually during 0–16 dpi and peaked at 16 dpi. Interestingly, SsPax3b showed no significant differences during the injury repair process, indicating that the satellite cells labeled by SsPax3b were not involved in muscle repair. These results imply that the muscle stem cell populations in teleosts are more complicated than in mammals. This lays the foundation for future studies on the molecular mechanism of indeterminant growth and muscle repair of large fish species.
Prognostic and Clinical Value of Cluster Analysis in Idiopathic Pleuroparenchymal Fibroelastosis Phenotypes
Authors: Nakamura Yutaro , Mori Kazutaka , Enomoto Yasunori , Kono Masato , Sumikawa Hiromitsu , Johkoh Takeshi , Colby Thomas V. , Yasui Hideki , Hozumi Hironao , Karayama Masato , Suzuki Yuzo , Furuhashi Kazuki , Fujisawa Tomoyuki , Enomoto Noriyuki , Inui Naoki , Kaida Yusuke , Yokomura Koshi , Koshimizu Naoki , Toyoshima Mikio , Imokawa Shiro , Yamada Takashi , Shirai Toshihiro , Nakamura Hidenori , Hayakawa Hiroshi , Suda Takafumi
Published: April 2021

Idiopathic pleuroparenchymal fibroelastosis (PPFE) is a distinctive interstitial pneumonia with upper lobe predominance that shows unique morphological features among idiopathic interstitial pneumonias (IIPs). Affected patients have a variety of clinical presentations with heterogeneous clinical courses. Cluster analysis is a valuable tool for identifying distinct clinical phenotypes under heterogeneous conditions. This study aimed to identify the phenotypes of patients with idiopathic PPFE. Using cluster analysis, novel PPFE phenotypes were identified among subjects from our multicenter cohort, and outcomes were stratified according to phenotypic clusters. Among the subjects with baseline data (N = 84), four clusters were identified. Cluster 1 included younger male subjects with coexisting non-UIP-like patterns. Cluster 2 included elderly female nonsmokers with low body mass index (BMI). Cluster 3 included elderly male smokers with a coexisting IP-like pattern. Cluster 4 included younger male smokers without lower lobe lesions. Patients in cluster 3 had significantly worse survival outcomes than those in clusters 1, 2, and 4 (p < 0.001, p = 0.0041, and p = 0.0155, respectively). Among idiopathic PPFE patients, cluster analysis using baseline characteristics identified four distinct clinical phenotypes that might predict survival outcomes.
Long-Term Clinical Outcomes and Carotid Ultrasound Follow-Up of Transcarotid TAVI. Prospective Single-Center Registry
Authors: Hudziak Damian , Hajder Adrianna , Gocol Radoslaw , Malinowski Marcin , Kazmierski Maciej , Morkisz Lukasz , Ciosek Joanna , Wanha Wojciech , Jarosinski Grzegorz , Parma Radoslaw , Darocha Tomasz , Wojakowski Wojciech
Published: April 2021

This study aimed to prospectively evaluate the safety and long-term clinical outcomes of cerebral-oximetry-guided transcarotid transcatheter aortic valve implantation (TC-TAVI) with systematic follow-up with carotid ultrasound. Thirty-three TCTAVI procedures were performed in our center from 2017 to 2019. Our analysis includes in-hospital outcomes and long-term follow-up data on mortality, echocardiographic parameters, carotid Doppler ultrasound, and VARC-2 defined clinical events. Intraoperatively, one patient died, and one had a transient ischemic attack (TIA). The following events occurred in-hospital postoperatively: myocardial infarction (3.0%), cardiac tamponade (3.0%), new-onset atrial fibrillation (6.3%), need for temporary pacing (27.3%) and need for pacemaker implantation (15%). The mean follow-up was 19.5 ± 9.52 months. In the long-term follow-up, the two-year survival rate was 83% ± 14. The echocardiographic parameters did not differ significantly from the postprocedural values, and the ultrasound did not show any cases of significant vessel narrowing. The mean peak systolic velocity (PSV) was 71.6 cm/s in the left common carotid artery and 70.6 cm/s in the right common carotid artery. In conclusion, cerebral oximetry-guided TC access is safe, has a favorable long-term outcome, and does not increase the risk of plaque formation in the carotid artery. In a carefully selected group of patients, it might be considered as a first-choice alternative to TF access.
Adverse Outcomes after Non-Cardiac Surgeries in Patients with Heart Failure: A Propensity-Score Matched Study
Authors: Lo Po-Han , Chang Chuen-Chau , Yeh Chun-Chieh , Sung Li-Chin , Cherng Yih-Giun , Chen Ta-Liang , Liao Chien-Chang
Published: April 2021

The impact of heart failure (HF) on postoperative outcomes is not completely understood. Our purpose is to investigate complications and mortality after noncardiac surgeries in people who had HF. In the analyses of research data of health insurance in, we identified 32,808 surgical patients with preoperative HF and 32,808 patients without HF undergoing noncardiac surgeries. We used a matching procedure with propensity score and considered basic characteristics, coexisting diseases, and information of index surgery between patients with and without HF. Adjusted odds ratios (ORs) and 95% confidence intervals (CIs) for complications and mortality after noncardiac surgeries in patients with HF were analyzed in multivariate logistic regressions. HF increased the risks of postoperative acute myocardial infarction (OR 2.51, 95% CI 1.99–3.18), pulmonary embolism (OR 2.46, 95% CI 1.73–3.50), acute renal failure (OR 1.97, 95% CI 1.76–2.21), intensive care (OR 1.93, 95% CI 1.85–2.01), and 30-day in-hospital mortality (OR 1.80, 95% CI 1.59–2.04). Preoperative emergency care, inpatient care, and injections of diuretics and cardiac stimulants due to heart failure were also associated with mortality after surgery. Patients with HF had increased complications and mortality after noncardiac surgeries compared with those without HF. The surgical care team may consider revising the protocols for perioperative care in patients with HF.
Pomalidomide Plus Low-Dose Dexamethasone in Relapsed/Refractory Multiple Myeloma Patients: Results of the Real-World “POWERFUL” Study
Authors: Terpos Evangelos , Repousis Panagiotis , Lalayanni Chrysavgi , Hatjiharissi Evdoxia , Assimakopoulou Theodora , Vassilopoulos Georgios , Pouli Anastasia , Spanoudakis Emmanouil , Michalis Eurydiki , Pangalis Gerassimos , Ntanasis-Stathopoulos Ioannis , Poziopoulos Christos , Kyrtsonis Marie-Christine , Pappa Vasiliki , Symeonidis Argiris , Georgopoulos Christos , Zikos Panagiotis M. , Gavriatopoulou Maria , Papadaki Helen A. , Dadakaridou Magdalini , Karvounis-Marolachakis Kiki , Katodritou Eirini
Published: April 2021

The “POWERFUL” multicenter, retrospective, and prospective study investigated the effectiveness of pomalidomide plus low-dose dexamethasone (POM/LoDex) therapy in relapsed/refractory multiple myeloma in routine care in Greece. Ninety-nine eligible adult patients treated with POM/LoDex according to the approved label after having received ≥2 prior therapies, including lenalidomide and bortezomib, were consecutively enrolled between 16 November 2017 and 21 February 2019 in 18 hematology departments. Fifty patients (50.5%) started POM/LoDex as third-line treatment. During the treatment period (median: 8.3 months; range: 0.3–47.6 months), the median POM dose was 4 mg/day, and 31.3% of the patients received additional antimyeloma agents. The overall response rate was 32.3%. During a median follow-up period of 13.8 months (Kaplan–Meier estimate), the median progression-free survival (PFS) was 10.5 months (95% CI: 7.4–14.4). The PFS was not significantly different between patients receiving POM/LoDex in the third versus later line of therapy, nor between patients receiving concomitant antimyeloma therapy versus POM/LoDEx doublet. During the prospective safety data collection period (median: 7.6 months) among patients with prospective follow-up (N = 75), POM-related adverse event incidence rate was 42.7% (serious: 18.7%; grade  ≥  3 hematological POM-related adverse events: 8.0%). Only neutropenia (13.3%) was reported at a frequency ≥10%. In conclusion, in this real-world study, POM/LoDex displayed a long PFS with no new safety signals emerging.
Inhibitors of Protein Convertase Subtilisin/Kexin 9 (PCSK9) and Acute Coronary Syndrome (ACS): The State-of-the-Art
Authors: Iannuzzo Gabriella , Gentile Marco , Bresciani Alessandro , Mallardo Vania , Di Lorenzo Anna , Merone Pasquale , Cuomo Gianluigi , Pacileo Mario , Sarullo Filippo M. , Venturini Elio , D’Andrea Antonello , Vigorito Carlo , Giallauria Francesco
Published: April 2021

Acute Coronary Syndrome (ACS) remains one of the most frequent causes of morbidity and mortality in the world. Although the age- and gender-adjusted incidence of ACS is decreasing, the mortality associated with this condition remains high, especially 1-year after the acute event. Several studies demonstrated that PCSK9 inhibitors therapy determine a significant reduction of major adverse cardiovascular events (MACE) in post-ACS patients, through a process of plaque modification, by intervening in lipid metabolism and platelet aggregation and finally determining an improvement in endothelial function. In the EVACS (Evolocumab in Acute Coronary Syndrome) study, evolocumab allows >90% of patients to achieve LDL-C < 55 mg/dL according to ESC/EAS guidelines compared to 11% of patients who only receive statins. In the EVOPACS (EVOlocumab for Early Reduction of low-density lipoprotein (LDL)-cholesterol Levels in Patients With Acute Coronary Syndromes) study, evolocumab determined LDL levels reduction of 40.7% (95% CI: 45.2 to 36.2; p < 0.001) and allowed 95.7% of patients to achieve LDL levels <55 mg/dL. In ODYSSEY Outcome trial, alirocumab reduced the overall risk of MACE by 15% (HR = 0.85; CI: 0.78–0.93; p = 0.0003), with a reduced risk of all-cause mortality (HR = 0.85; CI: 0.73–0.98: nominal p = 0026), and fewer deaths for coronary heart disease (CHD) compared to the control group (HR = 0.92; CI: 0.76–1.11; p = 0.38). The present review aimed at describing the beneficial effect of PCSK9 inhibitors therapy early after ACS in reducing LDL circulating levels (LDL-C) and the risk of major adverse cardiovascular events, which was very high in the first year and persists higher later after the acute event.
In Vivo Effects of Two In-Office Vital Tooth Bleaching Systems on Enamel Permeability
Authors: Bertacci Angelica , Ulian Gianfranco , Moro Daniele , Chersoni Stefano , Valdrè Giovanni
Published: April 2021

Tooth bleaching is a common treatment for the amelioration of the aesthetic of discoloured teeth. In this context, there are two common approaches that employ concentrated solutions (30–40 wt.%) of either hydrogen peroxide or carbamide peroxide as bleaching agents. However, there is an ongoing debate on the possible adverse effects of these different treatments on tooth health, such as variation of the enamel structure, surface morphology, and chemistry, which also affect tooth sensitivity. In the present work, a study on the effect of the two bleaching agents, a 35 wt.% solution of hydrogen peroxide and a 30 wt.% solution of carbamide peroxide, on the permeability and surface morphology of enamel is reported. The investigation was carried out on replicas of incisors obtained after different treatment times and for several patients, employing scanning electron microscopy to study the morphological features of the treated teeth. The significance of the analytical study was corroborated by a statistical analysis of the results. The collected data suggest that hydrogen peroxide treatment increases the enamel permeability, and this could be related with tooth sensitivity, whereas the carbamide peroxide solution increases the formation of precipitates on the tooth enamel.
Cooperative Maneuvering Mathematical Modeling for Multi-Tugs Towing a Ship in the Port Environment
Authors: Wu Gongxing , Zhao Xiaolong , Sun Yushan , Wang Linling
Published: April 2021

The towing operation of multi-tug-assisted ship navigation mainly relies on the experience of the captain, and there is no set of effective operation methods. Therefore, it is difficult to achieve accurate assisted navigation when multiple tugboats work in coordination. The calculation method of maneuverability of the towing system with multi-tug-assisted navigation is proposed in this paper. In view of the complexity of multi-tug-assisted large ship maneuvering, this article focuses on solving the problems of force analysis and maneuvering modeling between the multi-tug and ship systems. Firstly, a maneuvering mathematic model for towing ships is established, and the hydrodynamic force of the hull, rope force of the tugs, and force of wind interference are analyzed. The thrust and moment of the ducted azimuthal propeller are calculated, and the mathematical model of the tug’s cable tension is discussed. Then, the fourth-order Runge–Kutta method is used to solve the differential equations of the maneuvering motion of the ships and each tug. Based on the ship-towing process by multiple tugs, a multi-tug-assisted ship towing simulation platform was built by using the Visual Studio development tool. Finally, on the simulation platform, multi-tug longitudinal-towing-simulation experiments at different speeds were carried out, and the simulation of turning towing maneuvers under the influence of wind was done. The simulation results showed that as the towing speed increases, the initial towing speed fluctuates greatly. There is a significant drift effect on the ships by the wind force. And the wind will cause a fluctuation in the tug’s rope force. The simulation of the multi-tugs towing a ship entering the port was carried out in the port environment. The results showed that the multi-tug towing system and simulation platform may be used for the safety training of the tug’s crew.
Human Factor Issues in Remote Ship Operations: Lesson Learned by Studying Different Domains
Authors: Kari Raheleh , Steinert Martin
Published: April 2021

The idea of remote controlling ships for operational and commercial uses has developed beyond concepts. Controlling and monitoring vessels from a distant location requires updating the concept and requirements of shore control centers (SCCs), where human operators control the fleet via cameras, GPS, and many other types of sensors. While remote ship operation promises to reduce operational and maintenance costs, while increasing loading capacity and safety, it also brings significant uncertainty related to both the human-machine and human-human interactions which will affect operations. Achieving safe, reliable, and efficient remote ship operations requires consideration of both technological, cultural, social and human factor aspects of the system. Indeed, operators will act as captain and crew remotely, from the SCC, introducing new types of hardware and software interactions. This paper provides an overview of human factor issues that may affect human-machine and human-human interactions in the course of remote ship operations. In doing so, the literature related to remote operations in the domains of shipping, aerial vehicles, cranes, train transportation, automobiles, and mining is reviewed. Findings revealed that human factor issues are likely to fall into 13 distinct groups based on the type of human interactions that take place in SCCs.
Spatiotemporal Drought Risk Assessment Considering Resilience and Heterogeneous Vulnerability Factors: Lempa Transboundary River Basin in The Central American Dry Corridor
Authors: Khoshnazar Ali , Corzo Perez Gerald A. , Diaz Vitali
Published: April 2021

Drought characterization and risk assessment are of great significance due to drought’s negative impact on human health, economy, and ecosystem. This paper investigates drought characterization and risk assessment in the Lempa River basin in Central America. We applied the Standardized Evapotranspiration Deficit Index (SEDI) for drought characterization and drought hazard index (DHI) calculation. Although SEDI’s applicability is theoretically proven, it has been rarely applied. Drought risk is generally derived from the interactions between drought hazard (DHI) and vulnerability (DVI) indices but neglects resilience’s inherent impact. Accordingly, we propose incorporating DHI, DVI, and drought resilience index (DREI) to calculate drought risk index (DRI). Since system factors are not equally vulnerable, i.e., they are heterogeneous, our methodology applies the Analytic Hierarchy Process (AHP) to find the weights of the selected factors for the DVI computation. Finally, we propose a geometric mean method for DRI calculation. Results show a rise in DHI during 2006–2010 that affected DRI. We depict the applicability of SEDI via its relationship with El Nino-La Nina and El Salvador’s cereal production. This research provides a systematic drought risk assessment approach that is useful for decision-makers to allocate resources more smartly or intervene in Drought Risk Reduction (DRR). This research is also useful for those interested in socioeconomic drought.