bluuebunny/crossref_metadata_2025 huggingface dataset:
Análise do perfil clínico-epidemiológico de pacientes com síndromes aórticas agudas atendidos em serviço de referência cardiovascular no Pará
Authors: Ramos Gabriel Henrique de Paiva , Gustavo Kayo Silva , Barros Elisa Maria Novaes , Reis José Maciel Caldas dos , Barros Vanessa Novaes
Published: February 2024

Objetivo: Descrever o perfil clínico e epidemiológico de pacientes diagnosticados com síndromes aórticas agudas em um serviço de referência cardiovascular no município de Belém – Pará. Metodologia: Trata-se de um estudo descritivo de 12 pacientes admitidos no Hospital de Clínicas Gaspar Vianna, no período de março a dezembro de 2023. Foram consideradas variáveis relacionadas a idade, sexo, escolaridade, procedência, sintomas na admissão, característica da dor a admissão, presença de pulsos em membros superiores e inferiores, tipo de síndrome aórtica, classificação de Stanford e DeBakey, se foi necessário cirurgia, comorbidades, complicações, tempo de internação e desfechos. Resultados: A dor torácica esteve presente em todos os pacientes da amostra e em sua maioria foi súbita. Houve presença de pulso em MMSS e MMII. O tipo de síndrome aórtica mais frequente foi a Dissecção aguda. A classificação A de Stanford foi a mais frequente. Na classificação DeBakey houve maior proporção da classe I e III. Entre os participantes, 07 necessitaram de cirurgia, e a mais frequente foi a Bentall. Entre as comorbidades referidas, a HAS apresentou a maior proporção. A sepse foi a complicação mais frequente, seguida do LRA, da Hematese e da DHE. Conclusão: Metade dos pacientes tiveram como desfecho o óbito. São necessários mais estudos sobre esta patologia, assim como maior prevenção dos fatores de risco da doença para promover educação continuada a equipe de emergência e pronto socorro a fim de reduzir os óbitos e complicações de pacientes diagnosticados com esta síndrome.
PENGGUNAAN REWARD STICKER DALAM PENANAMAN SIKAP DISIPLIN ANAK
Authors: Hayati Miratul , Wafa Dian Rifatul
Published: February 2024

This study aims to explore the use of reward stickers in instilling discipline in early childhood. The research uses a qualitative approach using the case study method. The subjects in this study were TK B class teachers Waladun Solihun Tangerang Banten and TK B class children who used reward stickers in learning activities. We collected data through techniques, interview observations, and documentation. Data analysis using Miles Huberman with stages of data reduction, data presentation, verification and drawing conclusions. The results of the study explain that the use of reward stickers can be explored in (1) the type of reward given by the teacher in learning activities (2) the strategy in giving reward stickers (3) giving reward stickers in motivating children (4) aspects of discipline developed (5) response children in giving reward stickers (6) when giving reward stickers. This study recommends the use of reward stickers to improve children's discipline.
NILAI PENDIDIKAN KARAKTER DALAM KISAH YUSUF DI DALAM AL-QUR'AN DAN RELEVANSINYA DI ERA 4.0
Authors: Hidayah Nurul , Istiana Ila , Sulaikho Siti
Published: February 2024

Hancurnya moral disebabkan karena kurangnya pengajaran akhlak dan karakter di dunia pendidikan. Fokus Dalam pengajaran dalam dunia pendidikan dibidang kognitif dan psikomotorik, sedangkan pendidikan karakter merupakan pendidikan yang sangat penting diajarkan pada generasi milenial di era 4.0. Peneliti mengkaji tentang Nilai pendidikan karakter yang terdapat dalam kisah Yusuf didalam Al-qurâ€™an. Penelitian ini bertujuan untuk mengetahui nilai pendidikan karakter yang terdapat dalam kisah Yusuf. Metode yang digunakan adalah pendekatan kualitatif dengan jenis penelitian kepustakaan. Adapun teknik pengumpulan data menggunakan teknik dokumentasi, kemudian dianalisis menggunakan metode analisis isi. Hasil penelitian tentang nilai yang terdapat dalam kisah Yusuf adalah sabar dalam menghadapi perlakuan dzalim, kokoh dan tangguh dalam menghadapi godaan, cerdas dan jujur, pemaaf dan tidak pendendam, rasa kasih sayang, istiqomah dalam berdakwah, dan patuh kepada orang tua. Dan relevansi dari pendidikan karakter adalah pemuda yang sabar dan kuat, anak yang patuh kepada oaring tua, pemuda yang optimis dalam menghadapi masalah, tidak pendendam, serta pemimpin yang rendah hati.
Pembinaan Akhlak Melalui Pembiasaan Wudhu
Authors: Nurhayati Ety
Published: February 2024

By taking wudhu Islam teaches us to keep our healthy phisycally and spiritually, our mouth, nose, face, ears, hands to feet. Everything that Allah orders the moslem absolutely give purposes. Otherwise, taking wudhu is not only to clean our body phisycally but also our soul. When it is related to our soul so moral connect with this. By library data collection methode, read, take note and process the source are the serries actvivity of the lirary reasearch. Itâ€™s the researh that use library resource to get the research data and also describe many thing that related to all things to develop tha result. Habituation is the most important things in our daily activity because many people do activity because of the habit. Without the habit someoneâ€™s life will be so slow because he has to think first in doing something. All the habits could be formed through the stimulus, by the stimulus we hope that habit will be shown because the stimulation given before and because the activities that do more and more.
Pendidikan Islam Dalam Keluarga Perspektif Abdurrahman An-Nahlawi
Authors: Khoiroh Wakhlufli
Published: February 2024

The purpose of this study was to identify and examine the thoughts of Abddurrahman An-Nahlawi about Islamic Education in the Family. This research is a character study research or character thought study which is a type of qualitative research with a textual approach. Sources of data are reference books that are relevant to the issues discussed, the Qur'an and Hadith as well, especially the book by Abdurrahman An-Nahlawi entitled Ushulut Tarbiyah Islamiyah wa Asalibiha fil Baiti wal Madrasati wal Mujtama'. The data analysis technique is by examining the data sources and then critiquing, and comparing them. This study shows that the thought of Islamic Education in the Family According to Abdurrahman An-Nahlawi is very much needed in the world of education, especially in the family environment. The characteristics of Abdurrahman An-Nahlawi's thinking are guided by the Qur'an and Hadith. Every thought never eliminates the concept of human nature, it also has a clear educational goal, namely self-servitude to God.
Pengaruh Reward Dan Punishment Terhadap Kedisiplinan Siswa
Authors: Maspupah Hilmiyatul , Aprianif Aprianif
Published: February 2024

The purpose of this study was to find out how much influence reward and punishment have on student discipline, especially at MIS As-Syarifiyah Jatake Tangerang. This research uses quantitative methods, with double linear regression analysis, the study was conducted at MIS As-Syarifiyah, as for sampling techniques with random sampling. The analytical tests carried out include validity tests, reliability tests, classical assumption tests, hypothesis tests through t tests and f tests as well as coefficient of determination tests. The results of this study show that the variables X1Â  and X2 simultaneously affect student discipline, judging from the results of the f test (50.606 > 3.241) which showed that f count was greater than f table, then Ho was rejected and Ha was accepted and proven to be correct. Furthermore, the results of the t test to prove X1 and X2 have a partial effect on student discipline, and show the results of X1 with 4,735 > 2,026 and X2 with 3,803 > 2,026, then punishment affects discipline. The adjustend R square number of 0.732 shows 73.2%, which means that X1 and X2 have an effect on the discipline of students at MIS As-Syarifiyah, the remaining 26.8% is explained by other variables.
PENGARUH METODE DEMONSTRASI TERHADAP MINAT BELAJAR SISWA PADA PEMBELAJARAN FIQIH
Authors: Ningsih Sri Wahyuni , Diananda Amita
Published: February 2024

This research aimed to discover the effect of learning using the demonstration method on students' class X and XI learning interest in learning Fiqh (Islamic jurisprudence) at MA Nur As-Sholihat. This study used a descriptive quantitative correlational type and was conducted at MA Nur As-Sholihat, South Tangerang. The research subjects were students of class X and XI MA Nur As-Sholihat, totalling 100 students. The research applied a cluster sampling technique, and data collection techniques utilized questionnaires, interview and documentation. The validity result adopted the Pearson Product Moment correlation technique and the Cronbach Alpha Reliability Test with the help of SPSS 25. The data analysis used the Pearson Product Moment correlation and simple linear regression techniques. The results indicated a positive effect of the demonstration method on students' interest in learning Fiqh at MA Nur As-Sholihat. This can be seen from the acquisition of the t valueÂ  is 7,985. In contrast, the t table Â is 1,66, with a significance level is < 0,05. The criteria H0 Â is rejected if t valueÂ  ËƒÂ  t table Â at Î± = 5% with a value is 7,985 > 1,66. This means that the contribution of the variable X (Demonstration Method) with the variable Y (Students' Interest in Learning) is 39,4%, and the difference is 60,6% which was not examined in this research.
PERAN WALI KELAS DALAM MENERAPKAN DISIPLIN SISWA KELAS IX MTS DU’AUL FUQORO BOJONG BITUNG LEGOK TANGERANG
Authors: Nursari Nindi
Published: February 2024

This research was motivated by several problems such as students arriving late to school, students wearing uniforms that did not comply with school regulations and students chatting and joking in class when learning was taking place. The purpose of this study was to improve the discipline of class IX students at MTs Du'aul Fuqoro by applying an approach to homeroom teachers and class IX students. This research was conducted at MTs Du'aul Fuqoro Bojong Bitung Legok Tangerang. The subjects in this study were class IX students and homeroom teachers totaling about 96 students and also 3 homeroom teachers for class IX. This research is a qualitative research. The research was carried out in several stages including the planning and implementation stages. Data collection techniques using data triangulation in three ways, namely observation, interviews and documentation. Data analysis used the Miles and Huberman model. The results showed that the role of the homeroom teacher in applying discipline to students through the planning and implementation stages as well as data triangulation techniques by means of observation, interviews and documentation showed that the homeroom teacher of class IX was still unable to improve student discipline through the application of discipline. This happens because of several inhibiting factors in applying discipline to students, especially within the scope of the school, such as the lack of consequences given by the school for students who are not disciplined and also social factors
EFEKTIVITAS METODE UMMI DALAM MENINGKATKAN KEMAMPUAN MEMBACA AL-QUR’AN
Authors: Sholiha Nur Awaliatus , Tamimi Muhyiddin Tohir
Published: February 2024

This study aims to analyze the effectiveness of the Ummi method of learning the Koran in improving students' reading skills at the Mutiara Insani Islamic School Tangerang Middle School. This research is a qualitative descriptive study, using data and information through observation, interviews, and documentation. The data obtained was then processed by determining answers based on the results of interviews with the school principal, Ummi method coordinator, Ummi method AlQur'an teacher, and Mutiara Insani Middle School s tudents. This research is also a field research, namely direct observation of the object under study which aims to find relevant data related to learning the Qur'an using the Ummi method. The results of this study are that the Ummi method of learning the Qur'an at Mutiara Insani Middle School can be explained effectively and is able to improve the ability to read the Koran in students, because in learning the Ummi method emphasizes or prioritizes reading the Koran properly and correctly in accordance with the knowledge of tajwid.
EFEKTIVITAS KOMPETENSI PEDAGOGIK GURU PENDIDIKAN AGAMA ISLAM TERHADAP HASIL BELAJAR SISWA MATA PELAJARAN PAI
Authors: Tasliah Tasliah , Muslim Ahmad Buchori
Published: February 2024

The purpose of this study was to determine the Effectiveness of the Pedagogical Competence of Islamic Religious Education Teachers on the Learning Outcomes of Class VIII Islamic Religious Education Subjects at SMPN 1 Kresek Tangerang.This research is a research using descriptive qualitative method. The subjects of this study were the Principal of SMP Negeri 1 Kresek, Deputy Head of Curriculum for SMP Negeri 1 Kresek and students of SMP Negeri 1 Kresek. Methods of data collection by observation, interviews and documentation. Test the validity of the data through triangulation and then analyze the data using the techniques of data collection, data reduction, data display and conclusion drawing ferryfing.The results of this study indicate that the Pedagogical Competence of teachers at SMPN 1 Kresek is already classified as having fairly good competence and most of the students have met the Minimum Completeness Criteria (KKM) standards in learning outcomes in Islamic Religious Education subjects.
Study on evaporation drainage of deep coal seam gas wells
Authors: Zhu Hongying , Jing Chuankai , Zhang Fenna , Qi Yaoguang , Hu Hao , Yi Tiantian
Published: February 2024

Targeting the problem of a small amount of fluid accumulation in deep coal seam gas (CSG) wells during flowing production stage, the evaporation drainage method is proposed to discharge the liquid accumulation. Based on the Dalton evaporation model and wind speed function, a calculation model of evaporation drainage was established for deep CSG wells, which was verified by laboratory experiments. Taking a CSG well in the western Ordos Basin as an example to analyze the evaporation drainage capacity, the influence of temperature, daily gas production, bottomhole flowing pressure (BHFP), formation gas water saturation on the evaporation drainage capacity was investigated. The results show that the maximum evaporation water production is 2,533.8 kg/d at a bottomhole temperature of 80°C and a gas production rate of 30 × 103 m3/d. It is found that the temperature and pressure have a marked influence on the evaporation drainage. By improving the gas production and bottomhole temperature, and reducing the BHFP can effectively promote the evaporation drainage capacity. The initial moisture content of CSG in the reservoir are inversely proportional to the evaporation drainage capacity. By adjusting the BHFP and daily gas production, the evaporation drainage capacity can match the liquid production rate of the formation. Evaporation drainage can effectively extend the flowing production time of deep CSG wells and reduce the costs of production.
Double B-type magnetic integrated transformer based input-parallel output-parallel LLC resonant converter modules
Authors: Xia Nenghong , Yan Shuang , Ma Huaqi , Mao Xike , Chen Mengqi
Published: February 2024

The LLC resonant converter used in high-power situations suffers from the problems of high conduction loss and current stress, which can be solved using input-parallel output-parallel (IPOP)-connected converter modules. However, this leads to a multiple increase in the number of magnetic components, which reduces power density. Magnetic integration technology is an effective way to reduce the volume of converters. Currently, the magnetic integrated transformer based on EE-type cores is widely used to realize miniaturization, and it uses leakage inductance instead of resonant inductance to improve power density. However, leakage inductance is difficult to control, and the external radiated magnetic field will produce serious eddy current loss and electromagnetic interference. This article proposes a novel double B-type magnetic integrated transformer, which can integrate the magnetic components of two LLC resonant converters simultaneously and where the resonant inductances are wound independently. The structure contains four low reluctance branches, which are used as the cores of the transformer and the resonant inductance. The decoupling integration method, which integrates the four components into a single core, has been used to increase core utilization and improve power density. On this basis, the transformer’s high- and low-voltage windings are cross-arranged to reduce the magnetic field intensity in space, further decreasing the loss and electromagnetic interference. Compared with the EE-type magnetic integrated transformer, the volume of the proposed structure is reduced by 5.9%. A 400W experimental prototype is built, and the results verify the validity of the design.
CD200 genotype is associated with clinical outcome of patients with multiple myeloma
Authors: Gonzalez-Montes Yolanda , Osca-Gelis Gemma , Rodriguez-Romanos Rocío , Villavicencio Alicia , González-Bártulos Marta , Llopis Francesca , Clapes Victòria , Oriol Albert , Sureda Anna , Escoda Lourdes , Sarrà Josep , Garzó Ana , Lloveras Natàlia , Gómez Beatriz , Granada Isabel , Gallardo David
Published: February 2024

Immune dysfunction in patients with MM affects both the innate and adaptive immune system. Molecules involved in the immune response pathways are essential to determine the ability of cancer cells to escape from the immune system surveillance. However, few data are available concerning the role of immune checkpoint molecules in predicting the myeloma control and immunological scape as mechanism of disease progression. We retrospectively analyzed the clinical impact of the CD200 genotype (rs1131199 and rs2272022) in 291 patients with newly diagnosed MM. Patients with a CD200 rs1131199 GG genotype showed a median overall survival (OS) significantly lower than those with CC+CG genotype (67.8 months versus 94.4 months respectively; p: 0.022) maintaining significance in the multivariate analysis. This effect was specially detected in patients not receiving an autologous stem cell transplant (auto-SCT) (p < 0.001). In these patients the rs1131199 GG genotype negatively influenced in the mortality not related with the progression of MM (p: 0.02) mainly due to infections events.
Comprehensive bioinformatics analysis and systems biology approaches to identify the interplay between COVID-19 and pericarditis
Authors: Li Daisong , Chen Ruolan , Huang Chao , Zhang Guoliang , Li Zhaoqing , Xu Xiaojian , Wang Banghui , Li Bing , Chu Xian-Ming
Published: February 2024

Background

Increasing evidence indicating that coronavirus disease 2019 (COVID-19) increased the incidence and related risks of pericarditis and whether COVID-19 vaccine is related to pericarditis has triggered research and discussion. However, mechanisms behind the link between COVID-19 and pericarditis are still unknown. The objective of this study was to further elucidate the molecular mechanisms of COVID-19 with pericarditis at the gene level using bioinformatics analysis.

Methods

Genes associated with COVID-19 and pericarditis were collected from databases using limited screening criteria and intersected to identify the common genes of COVID-19 and pericarditis. Subsequently, gene ontology, pathway enrichment, protein–protein interaction, and immune infiltration analyses were conducted. Finally, TF–gene, gene–miRNA, gene–disease, protein–chemical, and protein–drug interaction networks were constructed based on hub gene identification.

Results

A total of 313 common genes were selected, and enrichment analyses were performed to determine their biological functions and signaling pathways. Eight hub genes (IL-1β, CD8A, IL-10, CD4, IL-6, TLR4, CCL2, and PTPRC) were identified using the protein–protein interaction network, and immune infiltration analysis was then carried out to examine the functional relationship between the eight hub genes and immune cells as well as changes in immune cells in disease. Transcription factors, miRNAs, diseases, chemicals, and drugs with high correlation with hub genes were predicted using bioinformatics analysis.

Conclusions

This study revealed a common gene interaction network between COVID-19 and pericarditis. The screened functional pathways, hub genes, potential compounds, and drugs provided new insights for further research on COVID-19 associated with pericarditis.
Combined repetitive transcranial magnetic stimulation and gut microbiota modulation through the gut–brain axis for prevention and treatment of autism spectrum disorder
Authors: Feng Pengya , Zhang Yangyang , Zhao Yonghong , Zhao Pengju , Li Enyao
Published: February 2024

Autism spectrum disorder (ASD) encompasses a range of neurodevelopmental conditions characterized by enduring impairments in social communication and interaction together with restricted repetitive behaviors, interests, and activities. No targeted pharmacological or physical interventions are currently available for ASD. However, emerging evidence has indicated a potential association between the development of ASD and dysregulation of the gut-brain axis. Repetitive transcranial magnetic stimulation (rTMS), a noninvasive diagnostic and therapeutic approach, has demonstrated positive outcomes in diverse psychiatric disorders; however, its efficacy in treating ASD and its accompanying gastrointestinal effects, particularly the effects on the gut–brain axis, remain unclear. Hence, this review aimed to thoroughly examine the existing research on the application of rTMS in the treatment of ASD. Additionally, the review explored the interplay between rTMS and the gut microbiota in children with ASD, focusing on the gut-brain axis. Furthermore, the review delved into the integration of rTMS and gut microbiota modulation as a targeted approach for ASD treatment based on recent literature. This review emphasizes the potential synergistic effects of rTMS and gut microbiota interventions, describes the underlying mechanisms, and proposes a potential therapeutic strategy for specific subsets of individuals with ASD.
VEGFR-3 signaling in macrophages: friend or foe in disease?
Authors: Kannan Saranya , Rutkowski Joseph M.
Published: February 2024

Lymphatic vessels have been increasingly appreciated in the context of immunology not only as passive conduits for immune and cancer cell transport but also as key in local tissue immunomodulation. Targeting lymphatic vessel growth and potential immune regulation often takes advantage of vascular endothelial growth factor receptor-3 (VEGFR-3) signaling to manipulate lymphatic biology. A receptor tyrosine kinase, VEGFR-3, is highly expressed on lymphatic endothelial cells, and its signaling is key in lymphatic growth, development, and survival and, as a result, often considered to be “lymphatic-specific” in adults. A subset of immune cells, notably of the monocyte-derived lineage, have been identified to express VEGFR-3 in tissues from the lung to the gut and in conditions as varied as cancer and chronic kidney disease. These VEGFR-3+ macrophages are highly chemotactic toward the VEGFR-3 ligands VEGF-C and VEGF-D. VEGFR-3 signaling has also been implicated in dictating the plasticity of these cells from pro-inflammatory to anti-inflammatory phenotypes. Conversely, expression may potentially be transient during monocyte differentiation with unknown effects. Macrophages play critically important and varied roles in the onset and resolution of inflammation, tissue remodeling, and vasculogenesis: targeting lymphatic vessel growth and immunomodulation by manipulating VEGFR-3 signaling may thus impact macrophage biology and their impact on disease pathogenesis. This mini review highlights the studies and pathologies in which VEGFR-3+ macrophages have been specifically identified, as well as the activity and polarization changes that macrophage VEGFR-3 signaling may elicit, and affords some conclusions as to the importance of macrophage VEGFR-3 signaling in disease.
The spectrum of neutralizing and non-neutralizing anti-FVIII antibodies in a nationwide cohort of 788 persons with hemophilia A
Authors: Oomen Ilja , Verhagen Marieke , Miranda Mariarosaria , Allacher Peter , Beckers Erik A. M. , Blijlevens Nicole M. A. , Bom Johanna G. van der , Coppens Michiel , Driessens Mariëtte , Eikenboom Jeroen C. J. , Fijnvandraat Karin , Hassan Shermarke , van Heerde Waander L. , Hooimeijer H. Louise , Jansen Joop H. , Kaijen Paul , Leebeek Frank W. G. , Meijer Daniëlle , Paul Helmut , Rijpma Sanna R. , Rosendaal Frits R. , Smit Cees , van Vulpen Lize F. D. , Voorberg Jan , Schols Saskia E. M. , Gouw Samantha C.
Published: February 2024

Objectives

Anti-factor VIII (FVIII) antibodies have been reported to exhibit both neutralizing and non-neutralizing characteristics. This is the first study investigating the full spectrum of FVIII-specific antibodies, including non-neutralizing antibodies, very-low titer inhibitors, and inhibitors, in a large nationwide population of persons with hemophilia A of all severities.

Methods

All persons with hemophilia A (mild (FVIII > 5–40 IU/dL)/moderate [FVIII 1–5 IU/dL)/severe (FVIII < 1 IU/dL)] with an available plasma sample who participated in the sixth Hemophilia in the Netherlands study between 2018 and 2019 were included. The presence of anti-FVIII antibodies of the immunoglobulin A, M, and G isotypes and IgG subclasses, along with antibody titer levels, were assessed using direct-binding ELISAs. FVIII specificity was assessed using a competition-based ELISA approach. The inhibitor status was determined using the Nijmegen ultra-sensitive Bethesda assay (NusBA) and the Nijmegen Bethesda assay (NBA).

Results

In total, 788 persons with hemophilia A (336 (42.6%) mild, 123 (15.6%) moderate, 329 (41.8%) severe hemophilia) were included. The median age was 45 years (IQR 24–60), and the majority (50.9%) had over 150 exposure days to FVIII concentrates. Within our population, 144 (18.3%) individuals had non-neutralizing FVIII-specific antibodies, 10 (1.3%) had very low-titer inhibitors (NusBA positive; NBA negative), and 13 (1.6%) had inhibitors (both NusBA and NBA positive). IgG1 was the most abundant FVIII-specific antibody subclass, and the highest titer levels were found for IgG4. In individuals without a reported history of inhibitor development, no clear differences were observed in antibody patterns between those who were minimally or highly exposed to FVIII concentrates. IgG4 subclass antibodies were only observed in persons with a reported history of FVIII inhibitor or in those with a currently detected (very low-titer) inhibitor.

Conclusion

In this cross-sectional study, we identified non-neutralizing antibodies in a relatively large proportion of persons with hemophilia A. In contrast, in our population, consisting of persons highly exposed to FVIII concentrates, (very low-titer) inhibitors were detected only in a small proportion of persons, reflecting a well-tolerized population. Hence, our findings suggest that only a small subpopulation of non-neutralizing FVIII-specific antibodies is associated with clinically relevant inhibitors.
Improved capabilities of global ocean reanalyses for analysing sea level variability near the Atlantic and Gulf of Mexico Coastal U.S.
Authors: Feng Xue , Widlansky Matthew J. , Balmaseda Magdalena A. , Zuo Hao , Spillman Claire M. , Smith Grant , Long Xiaoyu , Thompson Philip , Kumar Arun , Dusek Gregory , Sweet William
Published: February 2024

Realistic representation of monthly sea level anomalies in coastal regions has been a challenge for global ocean reanalyses. This is especially the case in coastal regions where sea levels are influenced by western boundary currents such as near the U.S. Atlantic Coast and the Gulf of Mexico. For these regions, most ocean reanalyses compare poorly to observations. Problems in reanalyses include errors in data assimilation and horizontal resolutions that are too coarse to simulate energetic currents like the Gulf Stream and Loop Current System. However, model capabilities are advancing with improved data assimilation and higher resolution. Here, we show that some current-generation ocean reanalyses produce monthly sea level anomalies with improved skill when compared to satellite altimetry observations of sea surface heights. Using tide gauge observations for coastal verification, we find the highest skill associated with the GLORYS12 and HYCOM ocean reanalyses. Both systems assimilate altimetry observations and have eddy-resolving horizontal resolutions (1/12°). We found less skill in three other ocean reanalyses (ACCESS-S2, ORAS5, and ORAP6) with coarser, though still eddy-permitting, resolutions (1/4°). The operational reanalysis from ECMWF (ORAS5) and their pilot reanalysis (ORAP6) provide an interesting comparison because the latter assimilates altimetry globally and with more weight, as well as assimilating ocean observations over continental shelves. We find these attributes associated with improved skill near many tide gauges. We also assessed an older reanalysis (CFSR), which has the lowest skill likely due to its lower resolution (1/2°) and lack of altimetry assimilation. ACCESS-S2 likewise does not assimilate altimetry, although its skill is much better than CFSR and only somewhat lower than ORAS5. Since coastal flooding is influenced by sea level anomalies, the recent development of skilful ocean reanalyses on monthly timescales may be useful for better understanding the physical processes associated with flood risks.
Case report: Management of recurrent pupillary optic capture with sutureless surgical technique using 7–0 polypropylene flange
Authors: Kim Dong Hyeon , Moon Da Ru Chi , Kang Yong Koo , Park Dong Ho , Shin Jae Pil , Kim Dai Woo , Do Jae Rock
Published: February 2024

Background

To report a novel surgical technique for recurrent pupillary optic capture after flanged intraocular lens (IOL) fixation.

Methods

In this retrospective case series, we detail our use of two parallel 7–0 polypropylene sutures passed between the iris plane and the optic of scleral-fixated IOL to address pupillary optic capture. Flanges were created using ophthalmic cautery to secure it to the sclera without suture.

Results

Two eyes with pupillary optic capture underwent a sutureless surgical technique using 7–0 polypropylene flanges. No recurrences of pupillary optic capture were observed during the 1-year follow-up.

Conclusion

Our sutureless surgical technique using a 7–0 polypropylene flange was an effective, efficient, and less invasive approach for treating recurrent pupillary optic capture.
Targeting host-specific metabolic pathways—opportunities and challenges for anti-infective therapy
Authors: Konaklieva Monika I. , Plotkin Balbina J.
Published: February 2024

Microorganisms can takeover critical metabolic pathways in host cells to fuel their replication. This interaction provides an opportunity to target host metabolic pathways, in addition to the pathogen-specific ones, in the development of antimicrobials. Host-directed therapy (HDT) is an emerging strategy of anti-infective therapy, which targets host cell metabolism utilized by facultative and obligate intracellular pathogens for entry, replication, egress or persistence of infected host cells. This review provides an overview of the host lipid metabolism and links it to the challenges in the development of HDTs for viral and bacterial infections, where pathogens are using important for the host lipid enzymes, or producing their own analogous of lecithin-cholesterol acyltransferase (LCAT) and lipoprotein lipase (LPL) thus interfering with the human host’s lipid metabolism.
Combined repetitive facilitative exercise under continuous neuromuscular electrical stimulation and task-oriented training for hemiplegic upper extremity during convalescent phase after stroke: before-and-after feasibility trial
Authors: Fujimoto Koya , Ueno Makoto , Etoh Seiji , Shimodozono Megumi
Published: February 2024

Introduction

Whereas repetitive facilitative exercise (RFE) affects primarily recovery of motor impairment after stroke, task-oriented training (TOT) focuses on facilitating daily use of the affected upper extremity. However, feasibility of combined RFE and TOT has not been reported. We originated “task-oriented RFE,” as a new combination therapy for patients with hemiplegic upper extremity after subacute stroke, to examine its feasibility in convalescent rehabilitation wards.

Methods

This is a before-and-after pilot study. Eight patients with hemiplegic upper extremity after subacute stroke received the task-oriented RFE program for 6 weeks at 80 min per day (20–60 min of TOT applied after 60–20 min of RFE under continuous neuromuscular electrical stimulation) in a convalescent rehabilitation ward. In the current program, we introduced the Aid for Decision-making in Occupation Choice (ADOC) iPad application as a goal-setting method for determining tasks. Feasibility was assessed with adherence to the protocol, adverse events in response to the intervention, and preliminary efficacy. Motor functions, amount of use and quality of movement in the hemiparetic upper extremity, and satisfaction of the patients were evaluated with Fugl-Meyer Assessment (FMA), the Action Research Arm Test (ARAT), the motor activity log (MAL) for the amount of use (AOU) and quality of movement (QOM) of the paralyzed hand, and ADOC.

Results

All participants accomplished the program, which was implemented as originally planned; neither nonattendance nor an adverse event occurred during the study. Favorable outcomes were obtained with all measures; mean changes in FMA, ARAT in the dominant hand, MAL-AOU, and MAL-QOM were greater than minimal clinically important differences. Mean changes in ADOC were greater than the minimal detectable change.

Discussion

The task-oriented RFE program was safe, well-tolerated, beneficial, and feasible within 80 min a day of occupational therapy, which means also within the procedural constraints of the Japanese health insurance system during the convalescent phase. Future studies are warranted to examine whether combined RFE and TOT enhances the efficacies of each program alone.
Acetylome analyses provide novel insights into the effects of chronic intermittent hypoxia on hippocampus-dependent cognitive impairment
Authors: Liu Fan , Yan Weiheng , Chen Chen , Zeng Yubing , Kong Yaru , He Xuejia , Pei Pei , Wang Shan , Zhang Ting
Published: February 2024

Introduction

Chronic intermittent hypoxia (CIH) can negatively affect hippocampal function through various molecular mechanisms. Protein acetylation, a frequently occurring modification, plays crucial roles in synaptic plasticity and cognitive processes. However, the global protein acetylation induced by CIH in the hippocampus and its specific effects on hippocampal function and behavior remain poorly understood.

Methods

To address this gap, we conducted a study using liquid chromatography-tandem mass spectrometry to analyze the lysine acetylome and proteome of the hippocampus in healthy adult mice exposed to intermittent hypoxia for 4 weeks (as a CIH model) compared to normoxic mice (as a control).

Results

We identified and quantified a total of 2,184 lysine acetylation sites in 1,007 proteins. Analysis of these acetylated proteins revealed disturbances primarily in oxidative phosphorylation, the tricarboxylic acid (TCA) cycle, and glycolysis, all of which are localized exclusively to mitochondria. Additionally, we observed significant changes in the abundance of 21 proteins, some of which are known to be associated with cognitive impairments.

Discussion

This study helps to elucidate the molecular mechanisms underlying CIH-induced changes in protein acetylation in the hippocampus. By providing valuable insights into the pathophysiological processes associated with CIH and their impacts on hippocampal function, our findings contribute to a better understanding of the consequences of CIH-induced changes in protein acetylation in the hippocampus and the potential role of CIH in cognitive impairment.
The role of microRNAs in acrylamide toxicity
Authors: Homayoonfal Mina , Molavizadeh Danial , Sadeghi Sara , Chaleshtori Reza Sharafati
Published: February 2024

The chemical compound known as Acrylamide (AA) is employed in different industries worldwide and is also found in thermal-processed food. AA has been acting as a reproductive toxicant, carcinogen, and neurotoxic in various animals, which may promote several toxic impacts in animal and human species. Up to now, various studies have focused on the harmful mechanisms and intervention actions of AA. However, the underlying mechanisms that AA and its toxic effects can exert have remained uncertain. MicroRNAs (miRNAs) are a class of short, non-coding RNAs that are able to act as epigenetic regulators. These molecules can regulate a wide range of cellular and molecular processes. In this regard, it has been shown that different chemical agents can dysregulate miRNAs. To determine the possible AA targets along with mechanisms of its toxicity, it is helpful to study the alteration in the profiles of miRNA regulation following AA intake. The current research aimed to evaluate the miRNAs’ mediatory roles upon the AA’s toxic potentials. This review study discussed the AA, which is made within the food matrix, the way it is consumed, and the potential impacts of AA on miRNAs and its association with different cancer types and degenerative diseases. The findings of this review paper indicated that AA might be capable of altering miRNA signatures in different tissues and exerting its carcinogen effects.
Prediction by a multiparametric magnetic resonance imaging-based radiomics signature model of disease-free survival in patients with rectal cancer treated by surgery
Authors: Mao Jiwei , Ye Wanli , Ma Weili , Liu Jianjiang , Zhong Wangyan , Yuan Hang , Li Ting , Guan Le , Wu Dongping
Published: February 2024

Objective

The aim of this study was to assess the ability of a multiparametric magnetic resonance imaging (MRI)-based radiomics signature model to predict disease-free survival (DFS) in patients with rectal cancer treated by surgery.

Materials and methods

We evaluated data of 194 patients with rectal cancer who had undergone radical surgery between April 2016 and September 2021. The mean age of all patients was 62.6 ± 9.7 years (range: 37–86 years). The study endpoint was DFS and 1132 radiomic features were extracted from preoperative MRIs, including contrast-enhanced T1- and T2-weighted imaging and apparent diffusion coefficient values. The study patients were randomly allocated to training (n=97) and validation cohorts (n=97) in a ratio of 5:5. A multivariable Cox regression model was used to generate a radiomics signature (rad score). The associations of rad score with DFS were evaluated using Kaplan–Meier analysis. Three models, namely a radiomics nomogram, radiomics signature, and clinical model, were compared using the Akaike information criterion.

Result

The rad score, which was composed of four MRI features, stratified rectal cancer patients into low- and high-risk groups and was associated with DFS in both the training (p = 0.0026) and validation sets (p = 0.036). Moreover, a radiomics nomogram model that combined rad score and independent clinical risk factors performed better (Harrell concordance index [C-index] =0.77) than a purely radiomics signature (C-index=0.73) or clinical model (C-index=0.70).

Conclusion

An MRI radiomics model that incorporates a radiomics signature and clinicopathological factors more accurately predicts DFS than does a clinical model in patients with rectal cancer.
Blended learning in nursing pharmacology: elevating cognitive skills, engagement and academic outcomes
Authors: Arien-Zakay Hadar
Published: February 2024

Pharmacological education is crucial for healthcare professionals to safely manage medications and reduce errors. Traditional lecture-based learning (LBL) often struggles to address this complexity, whereas newer methods, such as flipped classrooms and problem-based learning, yield mixed results, particularly in pre-clinical contexts, owing to students’ limited experience. Our nursing pharmacology course under LBL recorded a high failure rate of 37.8% and marginal passing scores across five cohorts (n = 849 students). An analysis using Bloom’s taxonomy revealed significant gaps in higher-order cognitive skills. As a remedy, the course was transformed into a novel blended learning format that integrated question-based learning (QBL) to enhance critical thinking across all cognitive levels. This model blends asynchronous and synchronous learning, is tailored to individual needs in large classes, and fosters continuous, student-centric learning. The redesign markedly decreased the failure rate by approximately 2.8-fold and increased the average grade by 11.8 points among 426 students. It notably improved the pass rates in advanced cognitive categories, such as “Evaluate” and “Create” by 19.0% and 24.2%, respectively. Additionally, the blended course showed increased student engagement, reflecting a dynamic and effective learning environment that significantly elevated participation and academic outcomes at all cognitive levels. This study demonstrated the profound impact of blended learning in pharmacology. By integrating QBL with various teaching methods, it surpasses traditional lecture-based limitations, enhancing engagement and understanding of complex topics by nursing students. Notable improvements in foundational and advanced learning suggest its broader application in health professionals’ education, effectively equipping students for clinical pharmacology challenges.
Effect of heterologous expression of FT gene from Medicago truncatula in growth and flowering behavior of olive plants
Authors: Guerrero Consuelo , Cerezo Sergio , Feito Isabel , Rodríguez Lucía , Samach Alon , Mercado José A. , Pliego-Alfaro Fernando , Palomo-Ríos Elena
Published: February 2024

Olive (Olea europaea L. subsp. europaea) is one of the most important crops of the Mediterranean Basin and temperate areas worldwide. Obtaining new olive varieties adapted to climatic changing conditions and to modern agricultural practices, as well as other traits such as biotic and abiotic stress resistance and increased oil quality, is currently required; however, the long juvenile phase, as in most woody plants, is the bottleneck in olive breeding programs. Overexpression of genes encoding the ‘florigen’ Flowering Locus T (FT), can cause the loss of the juvenile phase in many perennials including olives. In this investigation, further characterization of three transgenic olive lines containing an FT encoding gene from Medicago truncatula, MtFTa1, under the 35S CaMV promoter, was carried out. While all three lines flowered under in vitro conditions, one of the lines stopped flowering after acclimatisation. In soil, all three lines exhibited a modified plant architecture; e.g., a continuous branching behaviour and a dwarfing growth habit. Gene expression and hormone content in shoot tips, containing the meristems from which this phenotype emerged, were examined. Higher levels of OeTFL1, a gene encoding the flowering repressor TERMINAL FLOWER 1, correlated with lack of flowering. The branching phenotype correlated with higher content of salicylic acid, indole-3-acetic acid and isopentenyl adenosine, and lower content of abscisic acid. The results obtained confirm that heterologous expression of MtFTa1 in olive induced continuous flowering independently of environmental factors, but also modified plant architecture. These phenotypical changes could be related to the altered hormonal content in transgenic plants.
Association between type 2 inflammatory diseases and neurodevelopmental disorders in low-birth-weight children and adolescents
Authors: Huang Hengye , Zhang Kelvin Pengyuan , Sun Karol Kexin , Yu Guangjun
Published: February 2024

Background

Evidence of the association of certain neurodevelopmental disorder with specific type 2 inflammatory (T2) disease has been found. However, the association of various neurodevelopmental disorders with T2 diseases as a whole remains unclear in low-birth-weight (LBW) infants.

Objective

To evaluate the association of type 2 inflammatory (T2) diseases with intellectual disability (ID), autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), and learning disability (LD) in LBW children and adolescents.

Methods

The study sample was derived from 2005 to 2018 National Health Interview Survey sample child files. LBW children and adolescents aged 3–17 were included. History of T2 diseases (including asthma and atopic dermatitis) and four neurodevelopmental disorders were reported by adults in families. The relationship between T2 diseases and the risk of four neurodevelopmental disorders was investigated through multiple-weighted logistic regression. Age, sex, race/ethnicity, region, highest education in family and ratio of family income to the poverty threshold were adjusted as covariates for model estimation. Subgroup analyses were conducted by age stratification (3–11 and 12–17 years), sex (male and female), and race (white and non-white).

Results

11,260 LBW children aged 3–17 years [mean age (SE), 9.73 (0.05) years] were included, in which 3,191 children had T2 diseases. History of T2 diseases was associated with an increased risk of neurodevelopmental disorders, with an OR of 1.35 (95% CI, 0.99–1.84) for ID, 1.47 (95% CI, 1.05–2.05) for ASD, 1.81 (95% CI, 1.51–2.16) for ADHD, and 1.74 (95% CI, 1.49–2.04) for LD following the adjustment of all the covariates. The correlations between T2 disorders and each of the four neurodevelopmental disorders were significantly different by sex and race (all P for interaction < 0.001), and no differences were found in age stratification (all P for interaction > 0.05).

Conclusion

In a nationally representative sample of children, we found a significant association of T2 diseases with ASD, ADHD, and LD, even after adjusting for demographic baseline. We also found that the association of T2 disease with neurodevelopmental disorders differed between sex and race. Further investigation is needed to evaluate causal relationships and elucidate their potential mechanisms.
Metacognition in musical practices: two studies with beginner and expert Brazilian musicians
Authors: Araújo Rosane Cardoso de , Ferronato Rafael Stefanichen , Veloso Flávio Denis Dias
Published: February 2024

Metacognition is essential in the musical learning process as it involves understanding the purpose of each task, its planning, execution and evaluation. Considering the relevance of metacognitive processes, our objective in this study was to investigate how expert and beginner musicians manifest and verbalize their metacognitive processes in the context of preparing repertoire for a performance. The method used was a multi-case study carried out in two different contexts: with the five members of a brass quintet made up of professional musicians and with three beginner university violin students. The results obtained indicated that even at different levels of expertise, metacognitive processes were present in the musical practices of participants in the two contexts investigated. It was found that in both cases time management is a component of the preparation process in metacognitive regulation, however, for the beginner violinists in our sample it was a significantly more complex task than for the professional brass players. Regarding the learning monitoring and evaluation processes, it was possible to verify that beginner instrumentalists as well as professional musicians used declarative, conditional and procedural knowledge to carry out and reflect on their musical practices. These results have implications for both the individual and collective study process and for teaching processes. It is also observed that reflective thinking must accompany the processes of individual and collective interpretative-musical practices, considering that the musical results desired by musicians are related to the quality of cognitive, behavioral, affective and motivational undertakings pertinent to control and regulation of metacognitive processes.