bluuebunny/crossref_metadata_2025 huggingface dataset:
Silica's Medium in Various Concentration Effect on Navicula sp. Metabolism
Authors: Karillanata Khalid Erlangga , Luthfiana Dwi Hardianti , Maghfiroh Khusnul Qonita , Kurnianto Dedy , Amelia Ria , Erfianti Tia , Suyono Eko Agus
Published: September 2024

Abstract Navicula sp., an aquatic microalgae species, are numerous and diverse, with high metabolites so they hold great potential in biotechnology. Although it has many advantages, it is often not used in industry. In silica medium, Navicula sp. produces various metabolites depending on their concentration. This research aims to discover how adding silica to the medium affects the growth and production of Navicula sp. metabolites so that cultivation can be carried out at low cost and with maximum results. For 21 days, this experiment was carried out with three concentrations of silica (1; 1.5; and 2 mL/L) and one control (0 mL/L) grown each in a 500 mL culture of Navicula sp. Repetition was done thrice for each measurement parameter; growth speed, biomass production, lipids, carbohydrates, and protein. Medium silica 1.5 mL/L was the optimal concentration for growth speed, biomass production, and carbohydrate production for Navicula sp. (0.083; 0.54; and 0.075 mg/day, respectively). Meanwhile, the optimal silica concentration for lipid and protein production for Navicula sp. were 1 mL/L medium and control medium (0.517 and 0.8 × 10-2 mg/day, respectively). Overall, this research can be used to grow Navicula sp. in producing specific metabolites optimally. AbstrakNavicula sp., spesies mikroalga akuatik, sangat banyak dan beragam dengan metabolit yang tinggi, sehingga memiliki potensi yang besar dalam bioteknologi. Meskipun memiliki banyak keunggulan, spesies ini seringkali tidak digunakan dalam industri. Dalam medium silika, Navicula sp. menghasilkan berbagai metabolit tergantung pada konsentrasinya. Tujuan dari penelitan ini adalah untuk mengetahui bagaimana penambahan silika dalam medium memengaruhi pertumbuhan dan produksi metabolit Navicula sp., sehingga kultivasi dapat dilakukan dengan biaya yang rendah dan hasil yang maksimal. Selama 21 hari, eksperimen ini dilakukan dengan tiga konsentrasi silika (1; 1,5; dan 2 mL/L) dan satu kontrol (0 mL/L) yang ditumbuhkan di dalam 500 mL kultur Navicula sp. Pengulangan dilakukan tiga kali untuk setiap parameter pengukuran, yaitu kecepatan pertumbuhan, produksi biomassa, lipid, karbohidrat, dan protein. Medium silika 1,5 mL/L merupakan konsentrasi yang optimal untuk kecepatan pertumbuhan, produksi biomassa, dan produksi karbohidrat bagi Navicula sp. (0.083; 0.54;  dan 0.075 mg/hari, secara berurutan). Sementara itu, konsentrasi silika yang optimal untuk produksi lipid dan protein bagi Navicula sp., secara berurutan, adalah medium 1 mL/L dan medium kontrol (0.517 dan 0.8 × 10-2 mg/hari). Secara keseluruhan, penelitian ini dapat dijadikan sebagai solusi untuk menumbuhkan Navicula sp. dalam memproduksi metabolit tertentu secara optimal.
In Silico Evaluation Reveals The Potential Risk of Antimicrobial Resistance in Bifidobacterium
Authors: Rovik Anwar , Maulana Ahmad Reza , Aini Silmi Qurrotu , Kushayarti Dyah Fitri
Published: September 2024

AbstractBifidobacteria have beneficial health effects for their hosts. However, they may acquire antibiotic-resistance genes. They may transfer antibiotic-resistance genes to pathogenic microbes found in the human intestine resulting in the emergence of antibiotic-resistant pathogens. This study aimed to predict their resistance to antibiotics by analyzing the whole genome sequence. The entire genome data of Bifidobacterium spp. were obtained from the National Center for Biotechnology Information (NCBI). This study included five Bifidobacterium strains of human origin, five strains of animal origin, and three strains isolated from the environment. The genomic sequences were analyzed using ResFinder and CARD web service. Antibiotic-resistance genes were detected in Bifidobacterium spp. from all sample sources. Bifidobacteria were potentially resistant to various antibiotics, such as tetracycline, rifamycin, chloramphenicol, macrolide, lincosamide, streptogramin, and mupirocin-like antibiotics. This study suggests the safety aspect consideration of applying Bifidobacterium spp. as a potential probiotic.AbstrakBifidobacteria memiliki efek kesehatan yang menguntungkan bagi inangnya. Namun, sel Bifidobacteria dapat memperoleh gen resistensi antibiotik. Hal ini memunculkan potensi transfer gen resistensi antibiotik ke mikroba patogen yang ditemukan di usus manusia yang mengakibatkan munculnya patogen yang resisten terhadap antibiotik. Penelitian ini bertujuan untuk memprediksi resistensi Bifidobacteria terhadap antibiotik dengan menganalisis seluruh urutan genomnya. Seluruh data genom Bifidobacterium spp. diperoleh dari National Center for Biotechnology Information (NCBI). Penelitian ini melibatkan lima strain Bifidobacterium yang diisolasi dari manusia, lima strain yang berasal dari hewan, dan tiga strain yang diisolasi dari lingkungan. Sekuens genom dianalisis menggunakan ResFinder dan layanan web CARD. Gen resistensi antibiotik terdeteksi pada Bifidobacterium spp. dari semua sumber sampel. Bifidobacteria berpotensi menjadi resisten terhadap berbagai antibiotik, seperti tetrasiklin, rifamisin, kloramfenikol, makrolida, linkosamida, streptogramin, dan mupirocin-like antibiotics. Penelitian ini menyarankan pertimbangan aspek keamanan dalam menggunakan Bifidobacterium spp. sebagai probiotik potensial.
Anti-Inflammatory Effects of Velvet Bean Leaves Ethanolic Ointment (Mucuna pruriens L. (DC.)) on Mice Leukocytes Level
Authors: Fadilaturahmah Fadilaturahmah , Rahayu Resti , Santoso Putra
Published: September 2024

AbstractInflammation is a serious problem that needs to be treated. The use of steroid and non-steroidal drugs can treat inflammation well, but long-term use causes many side effects. So, it is necessary to use effective natural medicinal ingredients, one of which is velvet bean leaves (Mucuna pruriens L. (DC.)). This study aims to analyze the effect of velvet bean leaf ointment on the level of leukocyte components in mice experiencing inflammation. This research method was an experiment with a completely randomized design consisting of 6 treatment groups (normal, negative control, positive control (ketoconazole ointment)), velvet bean leaf ointment treatment at doses of 200, 400, and 600 mg/kgBW. The results showed that the use of velvet bean leaf ointment at doses of 200 and 400 mg/kgBW was able to significantly reduce the quantity of total leukocytes and monocytes in mice compared to the negative control (P <0.05); ointment at a dose of 200 mg/kgBW was able to significantly reduce the quantity of granulocytes and lymphocytes in mice compared to the negative control (P <0.05). The ability of velvet bean leaf ointment at a dose of 200 mg/kgBW is better in reducing the leukocyte quantity component compared to commercial drugs (ketoconazole ointment). Therefore, velvet bean leaf ointment at a dose of 200 mg/kgBW has great potential to be developed into an effective standardized anti-inflammatory drug.AbstrakInflamasi merupakan masalah serius yang perlu ditangani. Penggunaan obat steroid dan nonsteroid dapat mengatasi inflamasi dengan baik, namun penggunaan jangka panjang menimbulkan banyak efek samping. Maka dari itu perlu digunakan bahan obat alami yang efektif, salah satunya adalah daun kacang miang (Mucuna pruriens L. (DC.)). Penelitian ini bertujuan untuk menganalisis pengaruh salep daun kacang miang terhadap kadar komponen leukosit pada mencit yang mengalami inflamasi. Metode penelitian ini adalah eksperimen dengan rancangan acak lengkap yang terdiri dari 6 kelompok perlakuan (normal, kontrol negatif, kontrol positif (salep ketokonazol)), perlakuan salep daun kacang miang dosis 200, 400, dan 600 mg/kgBB. Hasil penelitian menunjukkan bahwa penggunaan salep daun kacang miang dosis 200 dan 400 mg/kgBB mampu menurunkan kuantitas total leukosit dan monosit mencit secara bermakna dibandingkan dengan kontrol negatif (P <0,05); Salep daun kacang miang dosis 200 mg/kgBB mampu menurunkan jumlah granulosit dan limfosit pada mencit secara signifikan dibandingkan dengan kontrol negatif (P <0,05). Kemampuan salep daun kacang miang dosis 200 mg/kgBB lebih baik dalam menurunkan komponen jumlah leukosit dibandingkan dengan obat komersial (salep ketokonazol). Oleh karena itu, salep daun kacang miang dosis 200 mg/kgBB memiliki potensi besar untuk dikembangkan menjadi obat antiinflamasi terstandar yang efektif.
Effect of EM4 (Effective Microorganism 4) on Growth and Productivity of Cucumber (Cucumis sativus L.)
Authors: Yuniati Ratna , Damayanti Margaretta Elsa , Wardhana Wisnu
Published: September 2024

AbstractCucumber (Cucumis sativus L.) is a nutritious and healthy vegetable that is commonly consumed by Indonesian people. To fulfill self-sufficiency for household scale needs during the COVID-19 pandemic, cucumber cultivation can be carried out in home gardens, using containers such as polybags. Growing cucumbers on limited land requires a carefully optimized planting media composition by applying Effective Microorganism 4 (EM4) to the polybag media when planting. The research has been conducted which aims to determine the best EM4 dosage for the growth and productivity of cucumbers. The study used a Randomized Block Design consisting of control and three treatment doses of 10% concentration EM4, namely 20, 40, and 60 mL per polybag with six replications. The planting media used is a mixture of loam soil and goat manure. NPK fertilizer is given as an additional nutrient. The EM4 application is done by pouring it every eight days into the planting media in polybags. The results showed an increase in growth parameters and productivity of cucumber plants namely plant height, leaf chlorophyll content, time of flower emergence, number of flowers, and number of flowers that form fruit. 40 mL EM4 is the dose that showed the highest growth and productivity.AbstrakMentimun (Cucumis sativus L.) merupakan sayuran bergizi dan menyehatkan yang banyak dikonsumsi masyarakat Indonesia. Untuk memenuhi swasembada kebutuhan skala rumah tangga di masa pandemi COVID-19, budidaya mentimun dapat dilakukan di pekarangan rumah, dengan menggunakan wadah polybag. Menanam mentimun di lahan terbatas memerlukan optimalisasi komposisi media tanam secara cermat dengan menerapkan Effective Microorganism 4 (EM4) pada media dalam polybag. Telah dilakukan penelitian yang bertujuan untuk mengetahui dosis EM4 terbaik untuk pertumbuhan dan produktivitas tanaman mentimun. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri atas kontrol dan tiga perlakuan dosis EM4 konsentrasi 10% yaitu 20, 40, dan 60 mL per polybag dengan enam ulangan. Media tanam yang digunakan adalah campuran tanah lempung dan kotoran kambing. Pupuk NPK diberikan sebagai unsur hara tambahan. Penerapan EM4 dilakukan dengan cara disiram setiap delapan hari sekali ke dalam media tanam di polybag. Hasil penelitian menunjukkan adanya peningkatan parameter pertumbuhan dan produktivitas tanaman mentimun yaitu tinggi tanaman, kandungan klorofil daun, waktu munculnya bunga, jumlah bunga, dan jumlah bunga yang membentuk buah. Dosis yang menunjukkan pertumbuhan dan produktivitas tertinggi adalah 40 mL EM4.
Management of Pyrrosia Mirb. (Polypodiaceae) Fern Specimens in Herbarium Depokensis (UIDEP) Universitas Indonesia, Depok
Authors: Atria Mega , Rahmandika Ghifari , Salamah Andi , Saifudin Saifudin , Putrika Afiatry
Published: September 2024

AbstractThe herbarium harbors collections of preserved plants used for scientific purposes. It is important to keep information about plants in a valid herbarium collection. This study aims to establish a valid collection and database of Pyrrosia stored in Herbarium Depokensis (UIDEP) through herbarium management. The management process includes taxonomic validation, specimen revitalization, and digitization. Taxonomic validation results showed there were 3 species of Pyrrosia namely P. piloselloides, P. lanceolata, and P. longifolia. These three species can be distinguished by rhizome scales, the shape of the lamina, and soral position. Physical examination throughout specimens showed several problems including loose specimen parts and specimen damage due to insects. Almost all collections (49 out of 59 specimens) did not include fertile lamina which is an important identifying character in Pyrrosia. To complete the herbarium data, 10 new Pyrrosia samples were collected at Green Spaces (RTHK) in Universitas Indonesia. All valid data and pictures undergo a digitization process. Those digitized data were then uploaded to the Herbarium UIDEP website (ruangkoleksibiotaui.id). This resulted in a Pyrrosia database that can be widely accessed by the public.AbstrakHerbarium menyimpan koleksi tanaman yang diawetkan yang digunakan untuk tujuan ilmiah. Penting untuk menyimpan informasi tentang tanaman dalam koleksi herbarium yang valid. Penelitian ini bertujuan untuk membangun koleksi dan database valid Pyrrosia yang disimpan di Herbarium Depokensis (UIDEP) melalui pengelolaan herbarium. Proses pengelolaannya meliputi validasi taksonomi, revitalisasi spesimen, dan digitalisasi. Hasil validasi taksonomi Pyrrosia menunjukkan terdapat 3 spesies, yaitu P. piloselloides, P. lanceolata, dan P. longifolia. Ketiga spesies ini dapat dibedakan berdasarkan sisik rimpang, bentuk lamina, dan letak soral. Pemeriksaan fisik pada seluruh spesimen menunjukkan beberapa permasalahan antara lain bagian spesimen yang lepas dan kerusakan spesimen akibat serangga. Hampir semua koleksi (49 dari 59 spesimen) tidak mencantumkan lamina subur yang merupakan karakter pengidentifikasi penting pada Pyrrosia. Untuk melengkapi data herbarium, dilakukan pengumpulan 10 sampel Pyrrosia baru di Ruang Terbuka Hijau (RTHK) Universitas Indonesia. Semua data dan gambar yang valid menjalani proses digitalisasi. Data digital tersebut kemudian diunggah ke website Herbarium UIDEP (ruangkoleksibiotaui.id). Hal ini menghasilkan database Pyrrosia yang dapat diakses secara luas oleh masyarakat.
Intra-articular Chondroma of the wrist: Imaging features of a rare salient entity
Authors: Kumar Manish , Singh Priya , Kumari Priti
Published: September 2024

This article presents a rare case of intra-articular chondroma of the wrist joint diagnosed with the help of MR arthrography.
Clinical characteristics associated with problematic pornography use among individuals seeking treatment for opioid use disorder
Authors: Stefanovics Elina A. , Kraus Shane W. , Madden Lynn M. , Farnum Scott , Cannata Erica , Potenza Marc N. , Barry Declan
Published: September 2024

Background and Aims

Many jurisdictions are experiencing opioid epidemics. Opioid use disorder (OUD) often co-occurs with other psychiatric disorders including behavioral addictions like gambling disorder. However, little is known regarding the frequency and correlates of problematic pornography use (PPU) among people seeking treatment for OUD. Here we aimed to investigate PPU and its correlates in people seeking OUD treatment.

Method

From October 2018 to March 2020, 1,272 individuals seeking OUD treatment were screened for PPU by completing the Brief Pornography Screen (BPS), a 5-item instrument validated for assessing PPU. Self-reported data were used.

Results

Among the sample there were 707 (60%) males and 565 (40%) females. The mean age of participants was 37.9 ± 10.5 years (range 18–73), there were 707 (60%) males and 565 (40%) females, 9.9% (n = 126) exhibited low positive BPS scores (1 ≤ score < 4), and 4.5% of the sample (n = 57) screened positive for PPU (BPS score ≥4). Individuals screening positive for PPU versus negative were mostly male (77%), scored higher on measures of impulsivity in the domains of positive urgency, negative urgency, and sensation-seeking and demonstrated more psychopathology on measures of substance use, psychotic symptoms, emotional lability, depression/functioning and self-harm.

Discussion and Conclusion

A minority of individuals seeking treatment for OUD screened positive for PPU. Among individuals with OUD, those screening positive (versus negative) for PPU were more impulsive and experienced more psychiatric symptoms, suggesting the need for additional investigation and screening for and addressing PPU in people with OUD.
The security and military context of the Russia–Ukraine war
Authors: Kis-Benedek József
Published: September 2024

One year of full-fledged conventional war fought on the European continent between two large countries, including a nuclear power, is obviously a historical watershed for NATO and the whole Euro-Atlantic area. The conflict has already caused many casualties, destroyed infrastructure and degraded the relationship between the West and Russia. The lecture summarizes the most important elements of the crisis from security and military perspectives.
Wykorzystanie podejścia sieciowego w tworzeniu organizacji uczącej się
Authors: Barczak Beata
Published: September 2024

The article presents the network approach and its importance in the development of the concept of the learning organisation. The paper presents the concept and characteristics of a learning organisation and characterises the network approach in management sciences. Particular attention is paid to the importance of knowledge and learning processes, and the use of IT in communication processes in network organisations and network perceptions of the learning organisation.
Kapitał ludzki przedsiębiorców jako kryterium decyzyjne w inwestycjach funduszy venture capital
Authors: Morawczyński Rafał
Published: September 2024

The article is a review of research on the importance of human capital for the assessment of entrepreneurs by venture capital funds. Theories that underpin the study of this problem - the theory of human capital theory, the theory of the firm and its chief executives - are the starting point. The characteristics of entrepreneurs, managers and their teams used to measure the quality of this capital in the decisions taken by venture capital are discussed, as are the conditions of this assessment, including the life cycle of the company, industry, country, and the uniqueness of the investment process and the relationship of these characteristics to the performance of firms. The paper also shows the evolution of research in this field, including the latest concepts from a psychological critique of the concept of bounded rationality. The article argues that human capital is rightly seen as one of the most important decision criteria used by venture capital funds.
SIMULACIONES EN PHET COMO ESTRATEGIA EN TIEMPOS DE COVID-19 PARA GENERAR APRENDIZAJE SIGNIFICATIVO AL POTENCIAR LA COMPETENCIA EXPLICACIÓN DE FENÓMENOS.
Authors: Rosero-Mellizo Luz Stella , Rivera-Toro Karol Adriana , Guerrero-Julio Marlene Lucila
Published: September 2024

The Francisco José de Caldas Educational Institution (IEFJC) of the municipality of La Sierra Cauca carried out adaptation processes of educational technologies to face the effects caused by social isolation due to COVID 19. In this sense, an investigation was carried out to develop a pedagogical strategy that will contribute to the generation of meaningful learning by enhancing the explanation of phenomena in chemistry by implementing simulations in PhET. The methodology had a mixed approach with a dominant model concurrent nested or embedded design (DIAC) applied to a group of 30 students out of eleven corresponding to the population and sample. The procedure was carried out in four phases with organized and structured activities under the modality of work at home during 20 weeks of the 2020 school year.The generation of significant learning in chemistry was considered as a dependent variable and the implementation of simulations in PhET and the pedagogical strategy as independent variables. The quantitative data analysis was done with a t-student test from the results obtained in the pretest and posttest tests that yielded a significant difference in each subcompetence before applying the strategy (51.2%) and after applying it (66.1%). On the other hand, the qualitative results were obtained from the analysis of what was recorded in the field diary and from the initial and final surveys evaluated with the Likert scale, where the students stated that the simulator contributed to their training and motivational process.The results of the evaluation of the impact of the strategy allowed to conclude that the research developed fulfilled the proposed objectives and allowed to validate the research hypothesis, that is, significant learning is generated by enhancing the explanation of phenomena in gaseous substances when using simulations in PhET.
Interactive Strategies for Media Promotion of South Korea Cultural Phenomena: Theoretical Aspects and Practical Experience
Authors:   Ural Federal University, Friesen Victoria V. , Glebovich Tatyana A. ,   Ural Federal University
Published: September 2024

The article considers the processes of media promotion of South Korean (East Asian) cultural industry phenomena. It analyses interactive promotion strategies that directly involve consumers in the content and contribute to its maximum popularisation. In the process of analysing theoretical approaches, it is revealed that many engagement strategies focus on creating fandoms as well as spreading a ‘participatory culture’. A critical analysis of practice identifies the most effective strategies for online and offline informants. Processes of running a telegram channel and organising small events that helped to establish unique audience feedback and draw conclusions about locally popular strategies are summarised.
Harlequinade Grotesque in the Works of Isak Dinesen (Based on the Novellas «The Poet» and «Carnival»)
Authors:   Ural Federal University, Tomberg Olga V.  , Kosareva Anna A. ,   Ural Federal University
Published: September 2024

The article is devoted to the study of the harlequinade grotesque in the novellas by the Danish writer Isak Dinesen (Karen Blixen) «The Poet» and «Carnival». The study proves relevant due to the following factors: 1) the current revival in foreign literary criticism of interest in the aesthetics of commedia dell’arte, as well as the role it played in the art of the 20th century; 2) growing interest in Dinesen’s work in Russian literary criticism. This article is the first to highlight both the presence of the harlequinade grotesque in Dinesen’s work and its originality. The authors of the article show how Dinesen’s passion for Italian popular comedy and harlequinade, typical of Scandinavian literature of her era, grew into a special worldview and writing style that synthesized the tragic and the comic. The article aims at revealing the features of the harlequinade grotesque in Dinesen’s work. The leading method in the study is the method of intertextual analysis: the characters and plots of the short stories are analyzed for their similarity with classical images (Harlequin, Pantaloon, Columbine, etc.) and collisions of the harlequinade (the flight of lovers from oppressive old men). The article reveals the features of the representation of the harlequinade plot and the functions of the harlequinade grotesque that evolve from them. The results presented in the article allow us to say that the harlequinade grotesque in Dinesen’s work was realized at the character and plot level, being the mouthpiece of the writer’s worldview, transmitting her aesthetic principles and moral principles. Also, the presence of the harlequinade grotesque in Dinesen’s prose suggests the presence of a similar technique in the work of other Scandinavian writers and confirms the status of this type of grotesque as a pan-European one.
Clinical profile of patients with non-ST segment elevation acute coronary syndrome: experience of a regional vascular center
Authors: Nesova A. K. , Tsydypova D. B. , Ryabov V. V.
Published: September 2024

Aim. To identify the main clinical and paraclinical characteristics of patients with non-ST segment elevation acute coronary syndrome (NSTE-ACS) of various risk groups for adverse ischemic events, as well as to describe the in-hospital outcomes of the disease.

Material and methods. The retrospective analysis included 600 patients who were hospitalized at the regional vascular center from January 2019 to January 2021 with a diagnosis of NSTE-ACS.

Results. Patients were stratified into very high — 208 (34,7%), high — 196 (32,7%), moderate — 108 (18%) and low — 88 (14,6%) risk groups. In the highand very high-risk groups, patients were older, characterized by a significant comorbidity index, increased bleeding risk, decreased left ventricular ejection fraction and glomerular filtration rate in comparison with patients of other groups (p<0,05). Very highrisk patients were characterized by associated clinical conditions, predominantly infectious diseases and decompensated heart failure. Unfavorable outcome of hospitalization was observed in 44 (7,3%) patients. The death probability in the very high-risk group (33; 15,9%) was higher than in the low-risk group (2; 2,3%) (Odds ratio 8,1 (95% confidence interval: 1,9-34,6); p=0,01). Regardless of the risk degree, there was a high (53,5%) likelihood of completing invasive coronary angiography as a diagnostic procedure. Most of the observed complications as a result of invasive coronary angiography were characteristic of the high(18,3%) and very high-risk groups (21,5%).

Conclusion. NSTE-ACS patients at very high/high risk of adverse ischemic events represent a heterogeneous group with cardiac and noncardiac pathologies with high comorbidity and bleeding risks, decreased left ventricular ejection fraction and glomerular filtration rate, and associated clinical conditions.
Cardiac manifestations in myotonic dystrophy type 1
Authors: Klementieva A. G. , Erokhina E. K. , Shamtieva K. V. , Melnik E. A. , Khrobostova  A. N. , Filipenko M. B. , Arakelyants A. A. , Peters T. V. , Pavlikova E. P.
Published: September 2024

Aim. To characterize cardiac damage in myotonic dystrophy type 1 (MD1), which is the most common form of hereditary primary muscular pathology in adults.

Material and methods. Forty-eight patients with MD1 (31 men, 17 women, mean age 39,2±9,3 years) underwent clinical examination, neuropsychological examination, lipid profile assessment, electrocardiography (ECG), Holter ECG monitoring, and echocar-diography. Four clinical cases are presented that demonstrate clear cardiac manifestations of MD1.

Results. The patients did not complain of arrhythmias or chest pain, while 7 (14,6%) had complaints of exercise shortness of breath. Cardiac conduction disorders occurred in 18 (37,5%) patients, which were represented by firstand second-degree atrioventricular (AV) block, his bundle and intraventricular conduction disturbances. According to Holter monitoring, heart rate <60 bpm was recorded in 46 (95,8%) patients, of which 2 (4,2%) were <30 bpm. One (2.1%) patient had atrial fibrillation. According to echocardiography, ventricular enlargement was detected in 3 (6,3%) patients, and atrial enlargement — in 8 (16,7%). None of the patients had an ejection fraction <50%.

Conclusion. Cardiac involvement is a common manifestation of MD1. Full cardiac examination can help not only improve the quality of life of patients, but also avoid possible side effects of the prescribed treatment.
Dynamics of residual shunt closure within one year after endovascular patent foramen ovale closure: a clinical study
Authors: Tereshchenko A. S. , Merkulov E. V.
Published: September 2024

Aim. To study 12-month dynamics of residual shunt (RS) closure after endovascular patent foramen ovale (PFO) closure.

Material and methods. We analyzed the data of 80 patients who underwent endovascular PFO closure at the Chazov National Medical Research Center for Cardiovascular Diseases in the period 2018-2023. All patients underwent a full range of examinations in accordance with current clinical guidelines. The severity of RS immediately after occluder implantation, as well as after 1, 6 and 12 months post-intervention assessment was based on transesophageal echocardiogram bubble study at the control visit.

Results. In the postoperative period, RS was visualized in 17,5% (n=14) of patients. Discharge was significant in 7,5% (n=6) of patients. One month after the intervention, residual significant discharge persisted in 10,0% (n=8) of patients. After 6 months, RS was visualized in 2,5% (n=2) of patients, and after 12 months, only in one patient (1,3%). The proportion of patients with RS 1 month after the intervention decreased to 42,9%, after 6 months to 85,7%, and after 12 months to 92,9% (p<0,05).

Conclusion. Most patients with RS after endovascular intervention for PFO are characterized by complete endocardialization of the occluder and PFO closure within the first year.
Potential and limitations of secondary prevention of noncommunicable diseases: data from a survey of Russian outpatient physicians
Authors: Mamedov M. N. , Kutsenko V. A. , Drapkina О. M.
Published: September 2024

Aim. To identify potential and limitations of secondary prevention of noncommunicable diseases in outpatient clinics of various Russian cities.

Material and methods. Outpatient physicians from 16 Russian cities took part in the cross-sectional study. A total of 545 general practitioners took part in the survey. The survey was carried out using a previously prepared questionnaire at the National Medical Research Center for Therapy and Preventive Medicine. It consisted of 11 blocks of questions, including sex and age characteristics, the prevalence of various diseases, adherence to therapy, preferential provision of medications, consultation assistance by hospital-based physicians and coverage of doctors and nursing staff. Answers were offered in several gradations to each question. Depending on the population, cities were divided into 4 following subgroups: population >2 million people (Moscow and St. Petersburg), population of 1-2 million people (Samara, Rostov-onDon, Krasnodar, Krasnoyarsk, Omsk), population of 0,4-1 million people (Vladivostok, Ulyanovsk, Penza, Kursk, Kirov, Tomsk) and population <400 thousand people (Nalchik, Saransk, Ivanovo).

Results. In all Russian cities, among men visited a clinic, 37% were people in the age range of 50-60 years. Second place was taken by men aged 60-65 years (30%). Most often, patients present with chronic coronary artery disease (39%), followed by type 2 diabetes (22,6%). Patients present with symptoms of heart failure in 17,7% of cases. Cancer account for 11,5% in the structure of visits. A similar frequency was found for chronic obstructive pulmonary diseases (9,2%). Every third patient is partially adherent to drug correction; partial non-adherence is detected 2 times less, while complete nonadherence is <3%. On average, 67% of doctors from different cities declare 75% achievement of target levels of risk factors, while one in five declare 50% achievement of recommended indicators. In most cases, physicians report minor problems during hospitalization. Every fifth doctor reported no problems, while every tenth respondent noted difficulties in hospitalizing patients. In every second case, partial staffing is noted. Moreover, a comparable trend is observed among doctors and nursing staff. In megacities and small towns the situation is somewhat better compared to cities with a population of 0,4 to 2 million.

Conclusion. To optimize the secondary prevention of noncommunicable diseases, a number of measures at the outpatient healthcare level should be taken. This includes educational work on adherence to drug and non-drug treatment correction, expanding coverage of preferential medication provision, improving interaction with hospitalbased doctors and coverage of doctors and nursing staff.
Gender-specific characteristics of heart failure with preserved ejection fraction in women: focus on pregnancy factors
Authors: Shperling M. I. , Mols A. A. , Kosulina V. M. , Abolmasov D. V. , Dzhioeva O. N. , Drapkina O. M.
Published: September 2024

The prevalence of heart failure with preserved ejection fraction (HFpEF) is relentlessly increasing, especially in women. Therefore, an important task is search for novel markers and early predictors of the disease. Evaluation of the association between physiological factors and complications of pregnancy and the development both early and longterm heart failure (HF) is also a substantial question. The younger women cohort is of particular interest because of the high prevalence of HFpEF among women in Russia. The purpose of the review is to assess gender differences in the HF, as well as to summarize the results of studies on the association of adverse pregnancy outcomes and development of HFpEF. The article describes the gender characteristics of the development of various HF types. We considered the morphofunctional, neurohumoral and age factors associated with the HF development in women, as well as the differences in the influence of the leading risk factors for HF between men and women. Particular attention is paid to the role of pregnancy factors in the development of various HF types. The following HF development variants in women have been identified: early (peripartum) and late (diagnosed in postmenopause). The pathophysiological mechanisms of the impact of extragenital pregnancy complications on HF development are described separately. Particular attention is paid to studying the relationship between pregnancy complications and HFpEF development in the long-term prognosis, as the most characteristic form of HF for women. The leading pregnancy complications associated with HFpEF development in postmenopause are hypertensive disorders (including preeclampsia and eclampsia) and gestational diabetes mellitus. A wider study of the association of pregnancy complications with HFpEF in a woman’s future is necessary to identify new mechanisms for its development.
Enhanced external counterpulsation in the management of patients with ischemic heart failure: long-term impact on clinical outcomes
Authors: Lishuta A. S. , Privalova E. V. , Belenkov Yu. N.
Published: September 2024

Aim. To study the long-term effect of complex therapy with enhanced external counterpulsation (EECP) on clinical parameters and outcomes in patients with stable coronary artery disease (CAD) complicated by heart failure (HF).

Material and methods. The randomized trial EXCEL (Long-term Effects of enhanced eXternal CountErpuLsation; NCT05913778) included 118 patients with stable CAD complicated by New-York Heart Association (NYHA) class II-III HF, who were randomized into group 1 (n=59) receiving optimal therapy and EECP (35 hours, 2 courses/ year) or group 2 (n=59) receiving optimal therapy and EECP (35 hours, 1 course/year). The 6-minute walk test (6MWT), clinical status, quality of life (MLHFQ questionnaire), N-terminal pro-brain natriuretic peptide (NT-proBNP) level, left ventricular ejection fraction, and clinical outcomes were assessed annually.

Results. In both groups, improvement of HF functional class, clinical status, exercise tolerance, NT-proBNP level, and quality of life were noted. Primary endpoint (increase in 6MWT distance >20%) in groups 1 and 2 was recorded in 100 and 79,7% of patients, respectively (p<0,001), while composite endpoint (myocardial infarction, revascularization, all-cause death, hospitalization for HF, new cases of atrial fibrillation, type 2 diabetes, stage 3-5 chronic kidney disease) in 18,6 and 52,5%, respectively. The odds ratio for primary and composite endpoint in group 1 was 0,207 (95% confidence interval: 0,090-0,475; p<0,001) and 0,032 (95% confidence interval 0,002-0,553; p=0,018).

Conclusion. The EXCEL study demonstrated a stable favorable effect of EECP in patients with HF complicated by CAD on clinical indicators and outcomes, which were significantly more pronounced in the group with 2 courses of EECP (70 hours) per year compared to a single annual course.
UMA ANÁLISE COMPARATIVA ENTRE A PARTICIPAÇÃO DE ESPECIALISTAS NA FORMULAÇÃO DE POLÍTICAS FUNDIÁRIAS NO BRASIL DE 1960 A 2009
Authors: De Oliveira Werneck de Capistrano Milena , Grisa Cátia
Published: September 2024

O artigo analisa iniciativas ao longo das últimas décadas para evidenciar cientificamente problemas e soluções à ocupação do território brasileiro. Evidências qualitativas foram coletadas em revisão documental e bibliográfica, entrevistas ou questionários e analisadas a partir de abordagens teóricas e conceituais das ciências políticas estadunidenses para comparar participações de especialistas em políticas fundiárias formuladas no Brasil entre 1960 e 2009. A análise evidencia que, desde o início da década de 1960, iniciativas conjuntas entre especialistas com expectativas em regular e redistribuir a propriedade da terra aos trabalhadores rurais resultaram, em distintas janelas de oportunidade, na Lei de Revisão Agrária do Estado de São Paulo, no Estatuto da Terra, na Associação Brasileira de Reforma Agrária, no Plano Nacional de Reforma Agrária (PNRA) da Nova República, na emenda da Campanha Nacional pela Reforma Agrária para a Constituinte e no PNRA do primeiro Governo Lula. Também mostra que, mesmo apesentando evidências científicas, especialistas com expectativas de regular e distribuir terras públicas ou devolutas não tiveram uma iniciativa conjunta na formulação do Programa Terra Legal no segundo Governo Lula. Se expectativas em limitar acessos, usos e funções da terra no Brasil influenciam participações relativas de especialistas em decisões governamentais, o artigo suscita inquietações – em ecos negacionistas e de fake news – sobre iniciativas conjuntas para evidenciar problemas e soluções à colonização do continente latino-americano.
ABORDAGENS DA HISTÓRIA DAS FACÇÕES CRIMINAIS DO RIO DE JANEIRO NA LITERATURA CIENTÍFICA: UMA REVISÃO DE ESCOPO
Authors: Kratochwill de Oliveira Thaíssa Fernanda , Silva Adriano , Constantino Patrícia
Published: September 2024

Esta revisão de escopo buscou investigar se e como a história das facções criminais oriundas do estado do Rio de Janeiro tem sido abordada na literatura científica. A pergunta de pesquisa foi: “como a história, percursos e desdobramentos da atuação das facções criminais oriundas do estado do Rio de Janeiro são descritos pela literatura científica?”. Para respondê-la, buscou publicações nas bases da Biblioteca Virtual em Saúde (BVS), SciELO, Scopus e Web Of Science. Foi adotado o procedimento duplo-cego para a seleção de publicações e o Guia PRISMA-ScR para sumarização dos resultados. Verificou-se que a história destas facções não foi objeto principal dos estudos, sendo abordada por eles de maneira breve e pontual. As formas de conceituá-las variavam desde as que enfatizavam sua atuação no tráfico; que destacavam seu caráter cultural; seu caráter de governança; seu caráter de resistência política. Quanto à história das facções, alguns trabalhos trouxeram dados sobre sua origem; outros sobre sua trajetória no mercado do tráfico; sobre o surgimento de uma “cultura de facção”; sobre a relação entre facções e Estado. As diferentes formas de conceituá-las e o destaque de certos aspectos de sua história em detrimento de outros seguem a tendência da literatura mais abrangente sobre o tema.
Effect of Financial Auditors’ Proficiency on Risk Management Process among Commercial Banks in Kenya
Authors: Kamau Charles Guandaru , Ombui Justin Orang’i
Published: September 2024

This study investigates the effect of financial auditors' proficiency on the risk management processes of commercial banks in Kenya. Given the increasing incidents of fraud and financial instability in the banking sector, this research examines the competencies, articulation, expertise, and prudence of financial auditors and their influence on risk management practices. Employing a descriptive research design and a positivist research philosophy, data were collected from a sample of 230 respondents across 39 commercial banks. The study reveals that auditors' proficiency significantly enhances risk identification, analysis, evaluation, and treatment. Moreover, the regulatory environment moderates the relationship between auditors' proficiency and the risk management process, emphasizing the need for regulatory compliance. These findings offer valuable insights for banks, regulatory bodies, and policymakers to improve risk management strategies. The study also provides a basis for further academic research and contributes to the existing literature on financial auditing and risk management.
Investigation of the effect of Beauveria bassiana (Balsamo) Vuillemin against potato beetle (Leptinotarsa decemlineata Say.) (Coleoptera: Chrysomelidae)
Authors: Yüceer Dr. Semiha
Published: September 2024

In this study, the objective was to determine the efficacy of entomopathogenic fungi on the Colorado potato beetle [Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae)]. To reach this goal, surveys were conducted in potato fields, and dead L. decemlineata and soil samples were collected to isolate entomopathogenic fungi. Pathogenicity tests were carried out using 30 entomopathogenic fungi that were obtained as a result of the analyses. According to the biological activity results, EP-1 isolate was identified and diagnosed as having 75% efficacy against L. decemlineata. Beauveria bassiana (EP-1), was identified as the most effective isolate. In the dose determination studies, 106, 107, and 108 conidia ml/l doses of B. bassiana's most effective isolate were used. The experiments were set up with five replications for each dose and control group. The spore suspension of the entomopathogenic fungus was sprayed on fully-grown potato plants cultivated in pots, targeting mature and 2nd or 3rd instar L. decemlineata. The number of live individuals was recorded on the 1st, 3rd, 5th, 7th, and 9th days after application to calculate the percentage of mortality. The most effective dose of B. bassiana isolate was determined to be 85% mortality on the 7th day after application with a dose of 108 conidia ml/l. According to the obtained data, it was observed that the mortality rates increased with the increase in dose on the 1st, 3rd, 5th, 7th, and 9th days after application. The highest impact was observed in applications with a dose of 108 conidia ml/l. In conclusion, the entomopathogenic fungus B. bassiana isolate, which is less harmful to humans and the environment, is considered suitable for use as a biological control agent against L. decemlineata.
New knowledge about Megachilidae and Halictidae (Hymenoptera: Apoidea) fauna from Diyarbakır and Bingöl provinces
Authors: Kaplan Emin , Güler Yasemin , Dikmen Fatih
Published: September 2024

The data about the bee fauna of Diyarbakır and Bingöl provinces of eastern Türkiye is quite limited. This study was conducted between 2016 and 2017 to determine Megachilidae and Halictidae (Hymenoptera: Apoidea) bee diversity of these two provinces. Adult samples were collected by atrap and killed with ethyl acetate. As a result of the evaluation of the collected bee samples, a total of 28 species (17 species from Megachilidae family and 11 species from Halictidae family) were identified from these provinces. Four species of them are known as cleptoparasites. In terms of the plant-bee relationship, eighteen of the species identified are polylectic, while five are oligolectic. Collection localities of all identified species were provided. With the current study, the number of known species belonging to the Megachilidae and Halictidae fauna of both provinces increased from 45 to 65.
Ecological and evolutionary implications of hybridization, polyploidy and apomixis in angiosperms
Authors: Cervantes-Díaz Cinthya I. , Oyama Ken , Cuevas Eduardo
Published: September 2024

Hybridization, polyploidy, and apomixis are related to the diversification of angiosperms, generating complex taxonomic relationships between species. Despite the differences in the frequencies of these phenomena, apomixis, the asexual seed production, tends to be frequently associated with hybridization and polyploidy. Apomixis has received more attention due to its potential to preserve desirable phenotypes in agriculture. The joint study of these three phenomena has been little explored, so the main objective of this review was to describe hybridization, polyploidy and apomixis and then analyze how they can be related. A synthesis of the main results of the studies obtained from a specialized literature search, the main patterns that address these three phenomena together, and their evolutionary and ecological consequences are presented.
Acknowledging Montessori Education — a Research Perspective of Montessori's Legacy for the Future
Authors: Ahlquist Eva-Maria Tebano , Courtier Philippine , Denervaud Solange , Gynther Per Wilhelm , Jendza Jaroslaw , Quarfood Christine
Published: September 2024

On 6 May 2022, 70 years after Maria Montessori’s death, Stockholm University and the Department of Education and Didactics organized an international Montessori symposium. The idea was to present a breadth of research on Maria Montessori. The symposium dealt with Maria Montessori in the interwar period, an analysis of the history of ideas. Another presentation suggested possible research models to study this large field. The symposium also presented interpretations of Montessori’s writings that point her out as a visionary and pioneer in education for a sustainable world. An additional research area addressed was the potential of neuroscience to examine the effects of teaching theory and learning in Montessori education. Finally, this report describes a study on whether Montessori-inspired education compared to traditional education stands up in areas of socio-economic disadvantage.
The Challenges and Opportunities of Implementing Montessori Education in the Public Sector
Authors: Fleming David J. , Culclasure Brooke Taylor , Warren Hannah , Riga Ginny
Published: September 2024

Over the past two decades, Montessori education in the United States has rapidly expanded from the private to the public sector. This expansion has created important questions about whether or not the Montessori approach can thrive alongside the education standards and accountability movement in the public sector. Questions also exist as to precisely who is benefiting from this expansion of and investment in public Montessori. To examine these topics, this study focused on South Carolina, the state with the highest number of public programs in the United States. We used implementation surveys, classroom observations, and teacher interview data collected by the research team and student record data collected by the state of South Carolina to conduct the analysis, which consisted of three parts. First, we investigated to what extent public programs in South Carolina are able to implement Montessori education with fidelity to the model. Second, we considered what program characteristics were related to higher levels of Montessori implementation fidelity. Third, we analyzed which children had access to higher-fidelity Montessori programs. Generally, findings indicated that, despite challenges created by the education standards and accountability movement and concerns expressed by educators about authenticity, most programs in South Carolina were implementing Montessori with fidelity. Several characteristics were associated with higher levels of fidelity, including the age of the program. Findings also indicated that Black, Hispanic, and students from low-income families were disproportionately participating in lower fidelity programs. Our study provides an in-depth analysis of the challenges and opportunities associated with government trying to implement successful private-sector education models in the public sector.
RNA Extraction CTAB Protocol v1
Authors: Joly Simon
Published: September 2024

Protocol used and fine-tuned in the Joly laboratory Reference Gambino G., Perrone I., Gribaudo I. 2008. A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochemical Analysis. 19:520–525.