bluuebunny/crossref_metadata_2025 huggingface dataset:
MicroRNA-200a improves diabetic endothelial dysfunction by targeting KEAP1/NRF2
Authors: Jiang Ziping , Wu Junduo , Ma Fuzhe , Jiang Jun , Xu Linlin , Du Lei , Huang Wenlin , Wang Zhaohui , Jia Ye , Lu Laijin , Wu Hao
Published: February 2020

Over a half of the diabetic individuals develop macrovascular complications that cause high mortality. Oxidative stress (OS) promotes endothelial dysfunction (ED) which is a critical early step toward diabetic macrovascular complications. Nuclear factor erythroid 2-related factor 2 (NRF2) is a master regulator of cellular antioxidant defense system and combats diabetes-induced OS. Previously, we found that impaired NRF2 antioxidant signaling contributed to diabetes-induced endothelial OS and dysfunction in mice. The present study has investigated the effect of microRNA-200a (miR-200a) on NRF2 signaling and diabetic ED. In aortic endothelial cells (ECs) isolated from C57BL/6 wild-type (WT) mice, high glucose (HG) reduced miR-200a levels and increased the expression of kelch-like ECH-associated protein 1 (Keap1) – a target of miR-200a and a negative regulator of NRF2. This led to the inactivation of NRF2 signaling and exacerbation of OS and inflammation. miR-200a mimic (miR-200a-M) or inhibitor modulated KEAP1/NRF2 antioxidant signaling and manipulated OS and inflammation under HG conditions. These effects were completely abolished by knockdown of Keap1, indicating that Keap1 mRNA is a major target of miR-200a. Moreover, the protective effect of miR-200a-M was completely abrogated in aortic ECs isolated from C57BL/6 Nrf2 knockout (KO) mice, demonstrating that NRF2 is required for miR-200a’s actions. In vivo, miR-200a-M inhibited aortic Keap1 expression, activated NRF2 signaling, and attenuated hyperglycemia-induced OS, inflammation and ED in the WT, but not Nrf2 KO, mice. Therefore, the present study has uncovered miR-200a/KEAP1/NRF2 signaling that controls aortic endothelial antioxidant capacity, which protects against diabetic ED.
СТРУКТУРНО-ФУНКЦІОНАЛЬНА МОДЕЛЬ СОЦІАЛІЗАЦІЇ ДИТИНИ В ПРОБЛЕМНІЙ СІМ’Ї: ЕМПІРИЧНИЙ ВИМІР
Authors: Skliaruk Anastasiia
Published: February 2020

У статті проаналізовано особливості соціалізації в проблемній сім’ї. Досліджується специфіка компонентів структурно-функціональної моделі соціалізації дитини в проблемній сім’ї за результатами емпіричного соціально-психологічного дослідження. Обґрунтовується запропонована модель соціалізації дитини в проблемній сім’ї, що відображає широкий спектр чинників (від загальносуспільного значення до рівня конкретних актів взаємодії в родині), що визначають успішність та ефективність соціалізації в проблемній сім’ї. Доводиться, що найбільш вагомими аспектами соціалізації в проблемній сім’ї є переважання типу репродукції сімейного досвіду та засвоєння типу конфігурації сімейних стосунків, що визначають позитивний чи негативний перебіг соціалізації дитини в проблемній сім’ї.
Екоморфічна структура фітоценозів на арені р. Дніпро (в межах природного заповідника «Дніпровсько-Орільський»)
Authors: Gudym N. G. , Ganzha D. S.
Published: February 2020

Проведено екоморфічний аналіз фітоценозів на арені р. Дніпро в межах природ-ного заповідника «Дніпровсько-Орільський». Оцінено екологічну специфіку фітоце-нозів, що є основою для розробки екологічно обґрунтованого їх збереження. Визначе-но, що у фітоценозах значну частину складають багаторічники. Проаналізовано це-номорфічну, трофоморфічну та гігроморфічну структуру фітоценозів. Різноманітнішляхи рознесення насіння призводять до формування значного потенціалу дистри-буції рослин у межах та за межами досліджуваного полігону.
Зміни анатомо-морфологічних показників рослин роду Аcer L. в умовах м. Дніпро
Authors: Pavlyukova N. F. , Legostaуeva T. V.
Published: February 2020

Вивчено видоспецифічні особливості анатомо-морфологічних показників одно-річних пагонів представників роду Acer L. Показано різницю параметрів гістологіч-них елементів пагонів дерев, що зростають на узбіччі автострад, та дерев з умовночистої зони. Виявлено пряму залежність між розмірами короку, первинної і вторин-ної кори та здатністю дерев протидіяти антропогенному впливу. Встановлено, що заанатомічною будовою найбільш толерантним до впливу техногенних емісій виявив-ся A. pseudoplatanus L., найбільш чутливим – A. negundo L.
Фітоценотичні позиції багаторічних злаків у степових та штучних лісових біотопах на правобережжі Присамар’я (Дніпропетровська область)
Authors: Mytsyk L. P. , Lisovets O. I. , Yakovenko V. M.
Published: February 2020

Показано участь багаторічних злаків, у тому числі за показником фітоцено-тичної активності, у травостої пробних площ, розташованих на правому березі рікиСамари від її берега до вододільних (плакорних) місцезростань. Всі згадані у статтівиди цієї групи рослин є потенційно цінними для практичного використання в умо-вах екологічної відповідності.
Активність каталази як показник інтродукції гібридогенних форм кісточкових в умовах Cтепового Придніпров’я
Authors: Kabar A. N. , Lykholat Yu. V. , Luchka Y. A. , Davydov V. R. , Boroday E. S.
Published: February 2020

Досліджено особливості функціонування каталази у міжвидових гібридів різ-них таксонів роду персик та мигдалю звичайного, що зростали в умовах ботанічногосаду Дніпровського національного університету імені Олеся Гончара.
Вплив техногенних умов зростання на зміни морфометричних показників однорічного пагона Ялини колючої
Authors: Iusypiva Т. , Zadesenets A. , Zloschastyeva D.
Published: February 2020

Вивчено дію викидів Придніпровської ТЕС (м. Дніпро) на морфометричні по-казники однорічного пагона Picea pungens Engelm (f. viridis Regel.). Виявлено, щовнаслідок хронічного впливу на рослини фітотоксикантів змінюється інтенсивністьросту пагонів і асиміляційного апарату. Запропоновано чутливі тест-параметри (ве-личина річного приросту пагонів, довжина і маса хвоїнки) для фітоіндикації станурослин ялини колючої в техногенних умовах зростання.
Coenofloras of deciduous shrubs (Fruticeta foliosa) and shrub steppes (Steppa fruticeta) in the Black Sea part of Inhulets river basin
Authors: Krasova O. O. , Korshykov I. I.
Published: February 2020

We revealed the composition of the coenotaxa of the shrub and shrub-steppe components of natural vegetation cover in the lower reaches of Inhulets river. For analyzing, we used 209 geobotanical releves collected during 1998–2017. Classification schemes of vegetation were created basing on the dominant approach. The article shows that the class of deciduous shrubs is presented by 9 associations belonging to 4 formations – Crataegeta fallacinae, Pruneta stepposae, Rhamneta catharticae, Roseta corymbiferae, and the class of shrub steppes – by 17 associations belonging to 6 formations – Amygdaleta nanae, Caraganeta fruticis, C. scythicae, Chamaecytiseta granitici, Ephedreta distachyae and Genisteta scythicae. The most widespread formation among deciduous shrubs is Crataegeta fallacinae, and among shrub steppes – Chamaecytiseta granitici. The last one determines the specifics of the vegetation cover in this region; its communities demonstrate high degree of adaptation to carbonate content in soils as well as tolerance to moderate total salt content, which is completely unusual for communities formed by ligneous biomorphes.

The specific richness of the coenoflora of deciduous shrubs (Fruticeta foliosa) is formed by 189 species, and one of the coenoflora of shrub steppes (Steppa fruticeta) by 261 species. The comparative analysis of the structures of the coenofloras showed their significant differences. The ranking order of families in the taxonomic spectrum of flora of shrub steppes, in its basic part, approaches to proportions which are peculiar to the flora of whole Northern Black Sea Area; the family Rosaceae occupying the second place in the similar spectrum for the class of deciduous shrubs makes this coenoflora closer to floras of Central Europe.  The characteristic feature of the flora of deciduous shrubs is the dominance of species of Palaearctic area type (25,8%), but, in the flora of shrub steppes, species of Black Sea area type (29,1%) prevail; it suggests that the core of the latter flora is original and indigenous. In contrast to the flora of deciduous shrubs where subshrubs are absolutely absent, the role of this biomorphe considerably increases within the composition of shrub steppes (up to 5,7%). The analysis of the ecologic structure of the investigated coenofloras showed that xeromesophytes prevail in the hygrospectrum of deciduous shrubs and euxerophytes do in the hygrospectrum of shrub steppes. In ecologic and coenotic spectra of both coenofloras, steppant species absolutely dominate: it is natural for shrub steppes and reflects the amphicoenotic character of deciduous shrubs.
Sеed productivity and quality of Picea abies and P. pungens in the Kriviy Rih plantations
Authors: Нuseіnova E. R.
Published: February 2020

Reproductive capability is considered as one of indicators of successful introduction; it helps to evaluate adaptation potential of plants, especially in techogenic conditions. The aim of our work is to research morphometric parameters of cones, profitability and seed quality of Рісеа аbies та P. pungens in the plantations with various levels of aerotechnogenic influence in conditions of an industrial city in the steppe zone of Ukraine. The objects of the studying were the cones of 30–40-year-old trees of P. аbies та P. pungens from eight different plantations situated along the whole city of Kryvyi Rih (126 km) and having various levels of technogenic pollution. We revealed that the maximal length of cone in two investigated species (108,4 and 88,7 mm respectively), width (28,6 and 24,7 mm); the highest seedling vigour (41,0 and 7,2 %), laboratory germination (54,2 and 20,6 %), seed mass (6,9 and 4,2 g), and length of germ (18,5 and 13,8 mm) are noted in the trees from Botanical Garden. The minimal values of all the same indices were ascertained for the trees growing near the metallurgical combine “ArcelorMittal Kryvyi Rih”: length of cones – 92,3 and 73,9 mm; width of cones – 26,2 and 22,4 mm; as well as the lowest seedling vigour (2,4 and 1,4 %); laboratory germination (5,0 and 2,0 %), seed mass (5,4 and 3,4 g), and length of germ (12,6 and 5,4 mm). The general seed quantity for a cone of P. аbies та P. pungens, in all the plantation types, varied from 203,9 to 217,2 seeds and from 198,6 to 204,3 ones respectively. The least part of fertile seeds (11,5–13,3 %), and the biggest part of sterile (56,5–58,7 %) and underdeveloped (29,8–30,1 %) ones were noted in the plants of P. pungens directly exposed to emissions of industrial enterprises; for the plants of P. abies the same indices were 20,2–22,5 %; 51,5–52,6 % та 26,0–27,2 % respectively. Therefore, increasing level of techogenic pollution by air pollutants, which is observed during some last years, oppresses the generative sphere of species of genus Рісеа in conditions of the steppe zone of Ukraine.
Surface covering on the floors of the Samara river area within the «Novomoskovsk Military Forestry» of the state enterprise
Authors: Vdovychenko V. N. , Ganzha D. S.
Published: February 2020

It was conducted an ecomorph analysis of plant communities in the arena of the Samara river. The vegetation cover of the territory of the State Enterprise «Novomoskovsky Military Forestry» in the areas of lower forest fires over the past 10 years was investigated and the process of pyrogenic successions was launched. Vegetation is an essential component of biogeocoenosis, which provides vital activity for other biotic components. Therefore, changes in vegetation under the influence of various environmental factors affect the state biogeocoenosis in general. Environmental analysis of plant communities allows qualitative assessment of plant communities and their comparison by composition of cenomorph, klimamorph, heliomorf, termomorf, trophomorf and hygromorf. It was estimated the environmental specific of phytocenosis communities, which is the basis for the development of their environmentally sound storage. It was determined that significant part of the phytocenosis communities is taken by perennials. The species composition of herbaceous plant communities is dominated by plants from the families Asteraceae and Poaseae. The presence of a large number of weeds demonstrates the instability of the ecosystem. In the presence of forest and weed-forest species of herbs. In dry conditions, steppe species are present. Rare plants such as Stipa borysthenica have been discovered. It is included in the red book of Ukraine. A detailed description of the flora and classification of plant communities according to the typology of A. Belgard was made. As a result of studies, it was established that grassy plants, mainly cereals, are actively mastering the ecological niche, which was freed up under the influence of a ground fire. Studies show certain patterns of pyrogenic successions and make it possible to predict such changes in other similar areas and carry out appropriate measures for reforestation, afforestation, care of forest crops and other forestry activities.
Karl Marx e a economia política: da rejeição moral à assimilação crítica
Authors: Wellen Henrique
Published: February 2020

Resumo: Buscou-se analisar, de forma breve, elementos históricos, teóricos e políticos que consubstanciaram o trânsito da relação entre Karl Marx e a economia política. Para tanto, destacou-se a relação dos sentidos das categorias de trabalho abstrato e de força de trabalho com o evolver dessa dinâmica, demarcado por três momentos centrais: uma crítica de teor filosófico e moral; a aceitação da teoria ricardiana dos salários; e uma assimilação crítica, propulsora de uma análise econômica inovadora.
O caráter ontológico da determinação social da saúde
Authors: Souza Diego de Oliveira
Published: February 2020

Resumo: O objetivo deste artigo é refletir sobre a ideia-chave trazida pelo debate acerca da determinação social da saúde, demonstrando seu caráter ontológico. Esclarecemos em que consiste uma abordagem ontológica. Em seguida, recuperamos proposições originais que apresentam a ideia de determinação social da saúde vinculada à dinâmica genérica do modo de produção. Por fim, fazemos uma crítica à teoria dos DSS, apontando seu caráter fragmentante, representando um retorno à abordagem gnosiológica.
Lamiaceae do Parque Estadual do Ibitipoca, Minas Gerais, Brasil
Authors: Mota Michelle , Pastore José Floriano Barêa , Marques Neto Roberto , Salimena Fátima Regina
Published: February 2020

Resumo Este estudo é um levantamento florístico da família Lamiaceae para o Parque Estadual do Ibitipoca. O Parque Estadual do Ibitipoca, inserido nos municípios de Bias Fortes, Lima Duarte e Santa Rita de Ibitipoca, no sudeste do estado de Minas Gerais, atualmente, é uma das Unidades de conservação mais visitadas do Brasil. Lamiaceae é uma das mais importantes famílias de Angiospermas, tanto economicamente quanto pela diversidade. Neste estudo foram encontrados 10 gêneros e 12 espécies: Aegiphila obducta, Cantinoa plectranthoides, Cyanocephalus rugosus, Eriope macrostachya, Hyptidendron asperrimum, Hyptis monticola, Mesosphaerum suaveolens, Rhabdocaulon coccineum, Salvia sellowiana, S. viscida, Vitex polygama e V. sellowiana. São apresentadas neste estudo, chave de identificação, descrição das espécies, ilustrações e comentários taxonômicos.
Flora da Reserva Ducke, Amazonas, Brasil: Sabiaceae
Authors: Amorim Bruno Sampaio , Cardozo Nállarett Dávilla , Fantin Cleiton , Albuquerque Patricia Melchionna , Cabral Fernanda Nunes
Published: February 2020

Resumo O presente trabalho tem como objetivo contribuir para o conhecimento das espécies de Sabiaceae ocorrentes na Reserva Ducke, Manaus - Amazonas. O estudo tem por base a análise morfológica de materiais depositados em herbários e revisão de literatura. Foram registradas duas espécies: Meliosma palustre e Ophiocaryon manausense. Chave para identificação, descrições, ilustrações, dados sobre hábitat, fenologia e distribuição geográfica das espécies são apresentados.
Ramet demography of Aechmea distichantha (Bromeliaceae) in two contrasting years in the understory and open areas of a South American xerophytic forest
Authors: Barberis Ignacio Martín , Klekailo Graciela , Albertengo Juliana , Cárcamo Juan Ignacio , Cárcamo José María , Galetti Luciano
Published: February 2020

Abstract The Schinopsis balansae forests of the Wet Chaco are characterized by convex areas with woody vegetation and plain areas with herbaceous vegetation. In the Wet Chaco, Aechmea distichantha is a terrestrial bromeliad that forms dense colonies in the understory and open areas of these forests. The aim of this study was to analyze the spatial and temporal variations in population dynamics of this bromeliad species. We monitored ramets growing in sun and shade conditions during two contrasting years. We analyzed the spatial and temporal variations in survival, flowering, and ramet production. Variations in survival, flowering, and ramet production were more marked between years than between habitats. During the year with wetter and milder temperature conditions, survival and ramet production were higher than during the drier year with more extreme temperatures. Survival of vegetative ramets was less variable than survival of young and reproductive ramets. In the colder year, lower winter temperatures reduced the populations in all stages, being more important in the open areas. Our results highlight the importance of low temperatures on A. distichantha demography at this xerophytic forest located at the southernmost distribution range of this bromeliad species.
Análisis de la lluvia de polen en un fragmento de bosque subandino en Colombia
Authors: Solano-Rodríguez Cristian Camilo , Mercado-Gómez Jorge David
Published: February 2020

Resumen Se llevó a cabo un análisis de la lluvia de polen y su relación con la vegetación en un fragmento de bosque subandino en la cordillera Oriental Colombiana entre los 1.500 a 2.400 msnm. Transectos tipo RAP fueron llevados a cabo para realizar el inventario de la flora. Dentro de cada transecto se recolectaron muestras de musgos de forma aleatoria, los cuales fueron procesados en el laboratorio, con el fin de obtener datos de lluvia de polen. Con base en los conteos e identificación de los granos de polen y vegetación, se realizaron diagramas polínicos, estimaciones de diversidad y fue establecida la lluvia de polen y su relación con la vegetación. Análisis Clúster y de correspondencia sin tendencia (DCA) fueron elaborados para determinar la presencia de asociaciones ecologicas de polen vinculados con la altura. Un total de 66 palinomorfos fueron identificados, siendo el hábito arbóreo dominante. Los sitios ubicados a 2.100 y 2.400 msnm presentaron mayor diversidad con respecto al polen. El Clúster y el DCA marcan tres agrupaciones compuestas por especies que generan una posible línea divisoria entre el bosque subandino bajo del alto, con lo cual se generan nuevos resultados que pueden ser usados en las interpretaciones paleoecológicas.
Renoprotective and Immunomodulatory Effects of GDF15 following AKI Invoked by Ischemia-Reperfusion Injury
Authors: Liu Jing , Kumar Sanjeev , Heinzel Andreas , Gao Michael , Guo Jinjin , Alvarado Gregory F. , Reindl-Schwaighofer Roman , Krautzberger A. Michaela , Cippà Pietro E. , McMahon Jill , Oberbauer Rainer , McMahon Andrew P.
Published: February 2020

Significance Statement

Gdf15, which encodes a signaling factor activated by oxidative stress, DNA damage, and proinflammatory cytokines, is upregulated in the human and mouse kidney within a few hours of ischemia-reperfusion injury. Using novel mouse strains, the authors mapped cellular sites of Gdf15 expression in normal and injured kidney and examined Gdf15’s role in ischemia-reperfusion injury. They showed that Gdf15 is expressed within hypoxic regions of the kidney and is predominantly activated within tubular epithelial cells at injury repair sites; loss of Gdf15 exacerbated injury, enhancing the inflammatory response. In an analysis of clinical data, they demonstrated that single nucleotide polymorphisms linked to lower circulating GDF15 levels associate with an increased incidence of biopsy-proven acute rejection. These findings point to modulating GDF15 levels in patients receiving kidney transplant as a possible therapeutic strategy.

Background

Gdf15 encodes a TGF-β superfamily member that is rapidly activated in response to stress in multiple organ systems, including the kidney. However, there has been a lack of information about Gdf15 activity and effects in normal kidney and in AKI.

Methods

We used genome editing to generate a Gdf15
nuGFP-CE mouse line, removing Gdf15 at the targeted allele, and enabling direct visualization and genetic modification of Gdf15-expressing cells. We extensively mapped Gdf15 expression in the normal kidney and following bilateral ischemia-reperfusion injury, and quantified and compared renal responses to ischemia-reperfusion injury in the presence and absence of GDF15. In addition, we analyzed single nucleotide polymorphism association data for GDF15 for associations with patient kidney transplant outcomes.

Results

Gdf15 is normally expressed within aquaporin 1–positive cells of the S3 segment of the proximal tubule, aquaporin 1–negative cells of the thin descending limb of the loop of Henle, and principal cells of the collecting system. Gdf15 is rapidly upregulated within a few hours of bilateral ischemia-reperfusion injury at these sites and new sites of proximal tubule injury. Deficiency of Gdf15 exacerbated acute tubular injury and enhanced inflammatory responses. Analysis of clinical transplantation data linked low circulating levels of GDF15 to an increased incidence of biopsy-proven acute rejection.

Conclusions

Gdf15 contributes to an early acting, renoprotective injury response, modifying immune cell actions. The data support further investigation in clinical model systems of the potential benefit from GDF15 administration in situations in which some level of tubular injury is inevitable, such as following a kidney transplant.
Factor atención de salud y la adherencia al consumo de multimicronutrientes en madres con niños de 6 –36 meses, que acuden al Centro de Salud Guadalupe, Juliaca – 2018
Authors: Mamani Tito Mayumy , Jarata Hancco Yovana
Published: February 2020

Objetivo la relación que existe entre el factor atención de salud y la adherencia al consumo de multimicronutrientes en madres con niños de 6 a 36 meses, Centro de Salud Guadalupe, Juliaca - 2018. Metodología: Es un estudio de tipo descriptivo correlacional, de enfoque cuantitativo, diseño no experimental y de corte transversal. La muestra fue no probabilística de tipo intencional, utilizando los criterios de inclusión y exclusión, como muestra se consideró a 70 niños de 6 a 36 meses de edad. Instrumento utilizado para la recolección de datos fue el Test de factor atención de salud y adherencia al consumo de multimicronutrientes de Lazarte (2017). Resultados encontrados indican en relación a la atención de salud 60% percibieron una mala atención, 24.35 percibieron una buena atención y solo 15.7% percibieron una atención regular. En cuanto a la adherencia 72.9% no son adherentes y 27.1% fueron adherentes al consumo de multimicronutrientes. Conclusión: Por lo tanto, existe una relación directa entre el factor atención de salud y la adherencia al consumo de multimicronutrientes, con un p-valor de 0. 000.Conclusión existe relación significativa entre ambas variables.
DEFICIENCIES OF PLATO’S DEMOCRATIC SYSTEM
Authors: Liashchenko Iryna
Published: February 2020

Main objective of the study. The objective of the study is to identify deficiencies of the democratic political system in order to protect democracy from rapid degradation into tyranny.
Methodology. The systematic method was selected as the methodological basis for the research, as it enables the consideration of Plato’s democratic system as interdependent with other types of states. The comparative method proved to be effective for distinguishing the characteristic features of the aristocratic, timocratic, oligarchic, democratic and tyrannical state of the human soul and Plato’s system of government.Findings and conclusions. To enable proper operation of democracy, it requires protection from its own deficiencies. These deficiencies include the following: firstly, the flaw of haughty, arrogant attitude towards wise talented naturally eminent people and the fear of their coming to power originates from timocracy; secondly, just as «barns with gold» destroyed the timocratic and oligarchic type of state, so the residues of these «barns» turn into a flaw of democracy in the form of a social abyss between the most affluent and the most deprived strata of the society; thirdly, this is excessive will in democracy that is gradually turning into excessive slavery. Regarding the latter, Plato emphasizes the anarchic extreme of freedom in democracy, which turns it into arbitrariness. After all, in a democracy there is no need to participate in government; not necessarily obey; no go to war; neither obey peace or laws, etc. The main consequence of all these deficiencies in the democratic system is the fostering of a future tyrant rooting from a people’s deputy. Since the thinker points out that no matter how many times a tyrant appears, he does not come from somewhere, but only from a democratic election procedure.
INTERACTION OF POWER AND SOCIETY WITHIN THE FRAMEWORK OF THE ASSOCIATION AGREEMENT BETWEEN UKRAINE AND THE EU AND EASTERN PARTNERSHIP POLICY PAPERS
Authors: Vnuchko Svitlana
Published: February 2020

Communication of public authorities and society, in particular, public authorities and separate social groups, is an important component of the political and managerial practice of modern society.
The world practice shows that effective communication between these actors provides citizens with free access to information, improves the efficiency of government and local self-government bodies, and creates a favourable environment for business development, which in turn serves as the basis for economic stability and the engine of the country’s social and economic development.
In the conditions of the development of the information society, new models, mechanisms, platforms of relations and communication between the authorities and the public are emerging. The constructive interaction between power structures and civil society organizations, with a «feedback» component, will contribute to meeting the needs and interests of citizens and strengthening the credibility of both power structures and civil society institutions. Moreover, it will improve the status of Ukraine at the international level by ensuring compliance with the Association Agreement and creating the preconditions for Ukraine to achieve the 20 key priorities of the Eastern Partnership 2020. In the future, consideration should be given to this issue in order to deepen, broaden and improve the effectiveness of the communication component of the government bodies and civil society organizations in Ukraine.
In the implementation of communication interaction, special attention should be paid to providing a feedback between communicators. After all, it is this component of the communications process that provides interaction and information about the society’s reaction to the results of the implemented decisions.
Purpose of the research: to conduct a scientific, theoretical and practical analysis of functioning of the existing in Ukraine communication platforms of civil society organizations and authorities in the framework of the Association Agreement and the Eastern Partnership policy papers.
LANGUAGE DEVELOPMENT AND SPEECH THERAPY SUPPORT: LITERATURE ANALYSIS
Authors: Vindece Aija , Usca Svetlana
Published: February 2020

Language disorders, including speech sound disorder, need to be diagnosed at the preschool age as they may jeopardize successful learning and socialization in the long term. Nowadays, there is a growing number of "late talkers", who need to provide speech therapy support at a preschool. The pedagogical tools available do not always meet the needs of today's children.The paper analyzes theoretical insights on the development of language as a complex system, correctional work, and the need to organize a transformative pedagogical and correctional work process that aims at developing the language system and reinforcing articulation of sound, using audiovisual stimulation as a unified system.
PECULIARITIES OF PSYCHOLOGICAL WELL – BEING YOUNG PEOPLE OF REZEKNE COMPREHENSIVE SCHOOLS
Authors: Kalvans Eriks
Published: February 2020

The problem of psychological well-being is particularly acute in the age group of young people, because during this time a person's identity is formed and the next profession is chosen. High psychological well-being implies the sense of purpose of life, optimism, the ability to establish positive attitudes with others, which creates preconditions for positive personality development. For this reason, young people from comprehensive schools were selected for the study.The significance of the research is justified by the fact that similar studies on psychological well-being in the youth age were not carried out in Latgale region.The aim of the study was to investigate the peculiarities of psychological well-being of young people in Rezekne comprehensive schools.
MSCs ARE LOCATED IN THE PERINEURIUM OF THE RECIPIENT RAT AFTER ALLOTRANSPLANTATION INTO DAMAGED NERVE
Authors: Petrova E S , Isaeva E N , Kolos E A , Korzhevskii D E
Published: February 2020

The gene and cell therapy, stimulating the regeneration of damaged nerves is currently under development. In these experiments mesenchymal stem cells (MSCs) are often used. The purpose of this study is to describe the localization and morphological features of mesenchymal stem cells derived from bone marrow after their allografting into the damaged rat nerve. MSCs of the bone marrow of Wistar-Kyoto rats were obtained from Transtechnology LLC (Head G.Polyntsev, Ph.D.). MSCs were cultured, identified and labeled by 5-bromo-2’-deoxyuridine (BrdU) in vitro. The sciatic nerve of adult Wistar-Kyoto rats (n = 12) was damaged (ligature, 40 sec), and the suspension of BrdU+ MSCs (5 · 104 cells in 5 μl per animal) was immediately transplanted into the damaged sciatic nerve. In a previous study, we have showed that some transplanted cells are located in the epineurium of the recipient’s nerve. The perineurium of the recipient rats was studied in the present work. Perineurial cells have polygonal form, thin and flat cell nucleus and form several layers, the basement membranes being placed between them. Perineurium is characterized by the presence of occluding junctions that can be identified using anti-claudine antibodies. The use of antibodies to claudine allowed us to identify perineurium. Some BrdU+ MSCs were found to survive 5-7 d following surgery and according to their localization and morphology became perineurium cells. Such extracellular matrix proteins as laminin, fibronectin, collagen are present in the perineurium. Apparently, the presence of these proteins creates a favorable biological environment for MSCs survival and for their differentiation towards perineurium cells. The results of the study confirm the mesenchymal origin of perineurium cells.
EFFECTS OF KARMIZOLE DERIVATE ON THE EXPRESSION OF APO A-I GENE IN THE RAT’S HYPERLIPIDEMIA MODEL
Authors: Lizunov A V , Okunevich I V , Shabanov P D
Published: February 2020

Azoles are the main antifungal drug class. The main mechanism of the azoles action is the intercalation in the sterol biosynthesis regulation. At the same time, the effect of the azole derivates on mammals is antiatherogenic. But there were no publication about connection between azole derivates effect on hyperlipidemia and expression of genes with antiatherogenic effects. In our work we used triton model of hyperlipodemia on rats to analyze the effect of carmizole injection on the expression of the main antiatherogenic genes and their regulators Apo A-I, HDL, LDL. We had four groups of rats: intact control group, triton control group, phenophibrate group and carmizole group. During a seven days we gave a per oral injections of carmizole for the carmizole group, phenophibrate (as a comparison drug) for phenophibrate group and 1% starch solution for triton control group. Liver tissue samples were used for RNA extraction and following RT-PCR (Real Time PCR) with primers for Apo A-I mRNA sequence. We have found, that Apo A-I mRNA level decreased in the triton control group to 17%, but restored up to 89% in the carmizole group. Carmizole derivate drug works like stimulator of Apo A-I gene expression. That increasing of the expression of antiatherogenic protein gene could me the base of the antiatherogenic effect of the carmizole derivate.
CYTOKINE PROFILE OF RAT BLOOD SERUM DURING EXPERIMENTAL BRONCHOPULMONARY INFLAMMATION AND IMMUNOMODULATOR ADMINISTRATION
Authors: Mokrenko E V , Shabanov P D
Published: February 2020

Experimental bronchopneumonia was modeled by administering turpentine to the trachea under ether anesthesia. After the operation, the solution of one of the studied immunomodulators was intraperitoneally administered to the animals for 5 days (1 time/day): polyoxidonium - 0.75 mg/kg, trekrezan 25 mg/kg or metaprot 25 mg/kg. After decapitation, the interleukin profile was determined in the blood using commercial kits (Merk). Inflammation decreased blood concentrations of both proinflammatory factors (IL-1β, IL-2, IL-12, INF-γ and chemokine MCP-1), and anti-inflammatory (IL-4, IL-10) cytokines. At the same time, the levels of TNFα and IL-6, as well as the anti-inflammatory cytokine IL-13, increased. The introduction of immunomodulators, polyoxidonium, trekrezan or metaprot substantially normalized the level of pro- (IL-1β, IL-2, IL-12 (p7), chemokine MCP-1, INFα) and anti-inflammatory cytokines (IL-4, IL-10). The anti-inflammatory effect of drugs is associated with the normalization of cytokine metabolism.
EXPERIMENTAL MODELING OF MULTIPLE SCLEROSIS: THE RESULTS OF CORRECTIVE ACTION OF ACTIVE ANTIOXIDANT
Authors: Okunevich I V
Published: February 2020

Comprehensive treatment of patients with multiple sclerosis (MS) is still relevant. The complex pathogenesis and acute incidence of MS in young people requires the use of multifunctional drugs with a protective effect, including antioxidants. The purpose of this study is to identify a corrective neuroprotective and immune modulating effect of the synthetic drug Triafen-2 (Trf-2), obtained in optimized way. Trf-2 was used at a dose of 50 mg/kg orally with chronic administration in II parts of experiments. To create an adequate model of MS - autoimmune encephalomyelitis (EAE), immunization of guinea pigs with an encephalitic mixture was used in Freund’s complete adjuvant. Mortality, a number of immunological parameters, the clinical picture of neurological complications and the severity of CNS damage in guinea pigs were determined. We studied morphologically the development of T-lymphocytic infiltration of the brain and spinal cord, demyelination of nerve fibers, the degree of cerebellar disorders, disorders of the pelvic organs, the presence of paresis and paralysis. A significant decrease in mortality was shown and protective effect of Trf-2 on neurological symptoms of EAE was noted. The reduction and even complete absence of paresis and paralysis in 66.5% of guinea pigs in the experimental group compared with animals that did not receive treatment was determined. A positive effect of Trf-2 on the migration activity of leukocytes and antibodies in the blood was found. In the group with Trf-2, there was a decrease in the content of lipid hydroperoxides and malonic dialdehyde. A pronounced neuroprotective and immune modulating effect of Triafen-2, which inhibits pathological disorders in an experimental model of EAE, has been established. The results justify the need for further study of Triafen-2 as a promising corrective drug.
MODERN BIOLOGICALLY ACTIVE SUBSTANCES STIMULATING SOMATIC CELLS AND FUNCTIONS OF NERVOUS AND IMMUNE SYSTEMS
Authors: Samuylenko A Ya , Grin S A , Shabunin S V , Raevsky A A , Miller R N , Melnik N V , Pavlenko I V , Albulov A A
Published: February 2020

With the help of BAS (biologically active substances), you can increase the ability of the cells of the body to maintain the constancy of its internal environment. The general condition improves, the prophylactic effect increases and mortality decreases, the weight gain in young animals grows. The cost of production is reduced.
ANALISIS FAKTOR YANG MEMPENGARUHI POLA KONSUMSI MI INSTANT PADA MAHASISWA DI UNIVERSITAS NEGERI MALANG
Authors: Katmawanti Septa , Ulfah Nurnaningsih Herya
Published: February 2020

ABSTRAK Antropologi gizi merupakan cabang atau spesialisasi dari antropologi kesehatan yang membahas secara khusus pada system budaya makanan serta kepentingan praktis dari kajian mengenai masalah gizi. Mi instan adalah salah satu contoh hasil kemajuan teknologi pangan yang banyak di konsumsi oleh warga Indonesia bahkan dunia, termasuk di Kota Malang. Universitas Negeri Malang merupakan universitas tertua di Kota Malang yang memiliki visi dan misi untuk menjadi universitas yang menjadi rujukan untuk pendidikan dan kesehatan. Tujuan penelitian: Mengembangkan analisis faktor-faktor yang mempengaruhi pola konsumsi mi instan pada mahasiswa di kota Malang. Metode Penelitian: Penelitian ini menggunakan metode cross sectional dengan pendekatan social budaya dalam ruang lingkup gizi masyarakat, dengan menggabungkan dua metode dalam pengumpulan data yaitu metode kualitatif dan metode kuantitatif. Hasil: 46% mahasiswa mengetahui mengenai pengolahan mi instan, faktor yang mempengaruhi pola konsumsi mi instan adalah pengetahuan, perilaku dan faktor sosial budayaKata kunci: mi instan, sosial budaya, mahasiswaABSTRACT Nutrition anthropology is a branch of health anthropology which specialising on the study of food culture and practical needs to a problem related to nutrition. Instant noodle is one example of food technology products which has been consumed by many Indonesian and other people around the world. Many students in Indonesia prefer to consume instant noodles. The State University of Malang as the oldest university in Malang has been a reference for many students to study in education and health fields. Research objectives: to analyse faktors influencing the pattern of instant noodles consumption among students in Malang. Research methodology: this research is a cross sectional research using sociocultural approach in nutritional community with mixed methode of quantitative and qualitative. Result: 46% of students understand the process of making instant noodles. The faktors influence the pattern of instant noodle consumption are knowledge, behaviour and sociocultural faktors. The students are knowledgable on the nutrition contained on instant noodle, the process of making it, and additional substance that can be add to the instant noodles. The students are also aware of the potential danger on continous consumption of instant noodles but not yet changed their behaviour of instant noodles consumption on daily life.Key words :instant noodle, social and cultural, students
EFEKTIVITAS PELATIHAN PEMBUATAN PMT MODISCO TERHADAP PENGETAHUAN IBU DI KELURAHAN TANJUNGREJO KECAMATAN SUKUN KOTA MALANG
Authors: Susanti Yeni , Devi Mazarina , Katmawanti Septa
Published: February 2020

Abstract: Modisco (Modified Dietetic Skim and Cotton Sheet Oil) is an additional food that contains high calories to increase the children's weights. The procedure to make PMT modisco is very easy because it consists of three basic ingredients which are skim milk, sugar and margarine. This study aims to know the effect of the training to the mother knowledge in making PMT modisco, the training was done by using lecture and demonstration method. The research design used is pre-experimental by using one group pretest and posttes method. The data for knowledge used questionnaire while the data. The samples in this study were all of the mothers who have Bawah Garis Merah (BGM) children in the village of Tanjungrejo Sub-district of Sukun District of Malang that were 11 people. The result is knowledge data using paired samplet t-test that obtained the score of Sig. (2-tailed) was 0.000 < 0.05 which means there is the effectiveness of training to the mothers’ knowledge related to PMT modisco. The procedure of making module PMT is not too difficult, there are three basic ingredients modisco in training abbreviated with milk, sugar, and margarine (SGM). Keywords: Training, Knowledge, PMT ModiscoAbstrak: Modisco (Modified Dietetic Skim and Cotton Sheet Oil) merupakan makanan tambahan yang mengandung kalori tinggi untuk meningkatkan berat badan anak. Prosedur pembuatan PMT modisco sangat mudah karena terdiri dari tiga bahan dasar yaitu susu skim, gula dan margarine. Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan terhadap pengetahuan Ibu tentang PMT modisco, pelatihan dengan metode ceramah dan demonstrasi. Jenis penelitian ini adalah pre-eksperimental dengan menggunakan desain one group pretest and posttest. Data pengetahuan menggunakan kuesioner, sampel penelitian ini adalah seluruh Ibu yang mempunyai anak Bawah Garis Merah (BGM) di Kelurahan Tanjungrejo Kecamatan Sukun Kota Malang sebanyak 11 orang. Hasil data pengetahuan menggunakan paired samplet t-test diketahui bahwa nilai Sig. (2-tailed) sebesar 0,000 < 0,05 artinya terdapat keefektivitasan pelatihan terhadap pengetahuan Ibu terkait PMT modisco. Prosedur pembuatan PMT modisco dirasa tidak terlalu susah, ada  tiga bahan dasar modisco yang dalam pelatihan disingkat dengan susu, gula, dan margarine (SGM).Kata kunci: Pelatihan, Pengetahuan, PMT Modisco
In silico characterization of linoleic acid biotransformation to rumenic acid in food derived Lactobacillus plantarum YW11
Authors: Aziz Tariq , Sarwar Abid , Fahim Muhammad , Al Dalali Sam , Ud Din Zia , Ud Din Jalal , Xin Zou , Jian Zhang , Pacheco Fill Taicia , Zhennai Yang
Published: February 2020

Lactobacillus plantarum YW11 capability to convert linoleic acid into conjugated linoleic acid and other metabolites was studied in a dose-dependent manner by supplementing LA at different concentrations. L. plantarum YW11 displayed a uniform distinctive growth curve of CLA and other metabolites at concentrations of LA ranging from 1% (w/v) to 10% (w/v), with slightly increased growth at higher LA concentrations. The biotransformation capability of L. plantarum YW11 evaluated by GC-MS revealed a total of one CLA isomer, i.e. 9-cis,11-trans-octadecadienoic acid, also known as the rumenic acid (RA), one linoleic acid isomer (linoelaidic acid), and LA metabolites: (E)-9-octadecenoic acid ethyl ester, trans, trans-9,12-octadecadienoic acid, propyl ester and stearic acid. All the metabolites of linoleic acid were produced from 1 to 10% LA supplemented MRS media, while surprisingly the only conjugated linoleic acid compound was produced at 10% LA. To assess the presence of putative enzymes, responsible for conversion of LA into CLA, in silico characterization was carried out. The in silico characterization revealed presence of four enzymes (10-linoleic acid hydratase, linoleate isomerase, acetoacetate decarboxylase and dehydrogenase) that may be involved in the production of CLA (rumenic acid) and LA isomers. The biotransformation ability of L. plantarum YW11 to convert LA into RA has great prospects for biotechnological and industrial implications that could be exploited in the future scale-up experiments.
Histopathological spectrum of neoplastic and non-neoplastic breast lesions: A three years study in rural tertiary care center.
Authors: Kumar Singh Dr. Sanjeev , Maurya Dr. Geeta , Pandey Dr. Pinki , Rashmi Dr.
Published: February 2020

Background: Breast tumours are very common cause of morbidity and mortality worldwide specially in developing countries. Breast lesions show broad spectrum of disease patterns in respect to benign, malignant, and non-neoplastic. Breast cancer is one of the most frequently occurring cancer among women.

Objective: To study the frequency, age distribution, clinical and histological patterns of neoplastic and non-neoplastic lesions of breast in this rural population.

Material and methods: This observational study was conducted over a duration of three years. In which we studied 210 cases of breast lesions. Specimens were received in department of pathology. Grossing, tissue processing, staining done according to standard protocol. Haematoxylin and eosin (H&E) stained sections were examined under microscope to categorise different type of lesions in the form of non-neoplastic and neoplastic.

Results:  Out of 210 breast lesions, fibroadenoma was the commonest (114 cases) benign lesion. While invasive ductal carcinoma (70 cases) was commonest in malignant category. Non neoplastic lesions stand on third place, study showed 6 cases out of 210 total cases, in which acute mastitis (4 cases) was most common. Benign tumours were mostly seen in 2nd and 3rd decade and malignant tumour mainly in 5th and 6th decade

Conclusion: Early histopathological diagnosis of breast lesions is very important to differentiate between benign and malignant lesions. Large number of cases of invasive ductal carcinoma (>33% of all cases) were seen in our study, which is a serious concern. So, generation of awareness among women is need of time to reduce the morbidity and mortality specially in Indian rural setup
Development of Correlation between Soaked CBR and In-Situ CBR Determined by DCP for Soil in the Southern Province of Rwanda
Authors: NTIRENGANYA Naphtal , Basher Babiker , Suliman Ahmed Elsir , Shallal Dr. Mohammed
Published: February 2020

In this paper, using Dynamic Cone Penetrometer (DCP), in-situ CBR tests have been carried out on 31 locations in Nyamgabe District in the Southern Province of Rwanda. The in-situ CBR results have been obtained from these tests. Thirty One tests pits were dug in the same locations and samples taken to the laboratory. Sieve Analysis, Atterburg Limits, Proctor and CBR tests were conducted on these samples. Accordingly a correlation has been developed between the In-Situ CBR using the DCP and the laboratory soaked CBR. The developed correlation helps to estimate the laboratory soaked CBR from the in-situ CBR using the DCP