bluuebunny/crossref_metadata_2025 huggingface dataset:
Omics Studies Revealed the Factors Involved in the Formation of Colony Boundary in Myxococcus xanthus
Authors: Anwar Mian Nabeel , Li Zhi Feng , Gong Ya , Singh Raghvendra Pratap , Li Yue-Zhong
Published: June 2019

Two unrecognizable strains of the same bacterial species form a distinct colony boundary. During growth as colonies, Myxococcus xanthus uses multiple factors to establish cooperation between recognized strains and prevent interactions with unrecognized strains of the same species. Here, ΔMXAN_0049 is a mutant strain deficient in immunity for the paired nuclease gene, MXAN_0050, that has a function in the colony-merger incompatibility of Myxococcus xanthus DK1622. With the aim to investigate the factors involved in boundary formation, a proteome and metabolome study was employed. Visualization of the boundary between DK1622 and ΔMXAN_0049 was done scanning electron microscope (SEM), which displayed the presence of many damaged cells in the boundary. Proteome analysis of the DK1622- boundary disclosed many possible proteins, such as cold shock proteins, cell shape-determining protein MreC, along with a few pathways, such as RNA degradation, phenylalanine, tyrosine and tryptophan biosynthesis, and Type VI secretion system (T6SS), which may play major roles in the boundary formation. Metabolomics studies revealed various secondary metabolites that were significantly produced during boundary formation. Overall, the results concluded that multiple factors participated in the boundary formation in M. xanthus, leading to cellular damage that is helpful in solving the mystery of the boundary formation mechanism.
Specifically Designed Ionic Liquids—Formulations, Physicochemical Properties, and Electrochemical Double Layer Storage Behavior
Authors: Yue Zheng , Ma Qiang , Mei Xinyi , Schulz Abigail , Dunya Hamza , Alramahi Dana , McGarry Christopher , Tufts Jim , Chakrabarti Amartya , Saha Rituparna , Mandal Braja K.
Published: June 2019

Two key features—non-volatility and non-flammability—make ionic liquids (ILs) very attractive for use as electrolyte solvents in advanced energy storage systems, such as supercapacitors and Li-ion batteries. Since most ILs possess high viscosity and are less prone to dissolving common electrolytic salts when compared to traditional electrolytic solvents, they must be formulated with low viscosity thinner solvents to achieve desired ionic conductivity and dissolution of electrolyte salts in excess of 0.5 M concentration. In the past few years, our research group has synthesized several specifically designed ILs (mono-cationic, di-cationic, and zwitterionic) with bis(trifluoromethylsulfonyl)imide (TFSI) and dicyanamide (DCA) as counter anions. This article describes several electrolyte formulations to achieve superior electrolytic properties. The performance of a few representative IL-based electrolytes in supercapacitor coin cells is presented.
Ergonomic Challenges Inherent in Neonatal Resuscitation
Authors: Yamada Nicole K. , Fuerch Janene H. , Halamek Louis P.
Published: June 2019

Neonatal resuscitation demands that healthcare professionals perform cognitive and technical tasks while working under time pressure as a team in order to provide efficient and effective care. Neonatal resuscitation teams simultaneously process and act upon multiple data streams, perform ergonomically challenging technical procedures, and coordinate their actions within a small physical space. An understanding and application of human factors and ergonomics science broadens the areas of need in resuscitation research, and will lead to enhanced technologies, systems, and work environments that support human limitations and maximize human performance during neonatal resuscitation.
Cry Protein Crystal-Immobilized Metallothioneins for Bioremediation of Heavy Metals from Water
Authors: Sun Qian , Cheng Sze Wan , Cheung Kelton , Lee Marianne M. , Chan Michael K.
Published: June 2019

Cry proteins have been the subject of intense research due to their ability to form crystals naturally in Bacillus thuringiensis (Bt). In this research we developed a new strategy that allows for the removal of cadmium and chromium from wastewater by using one Cry protein, Cry3Aa, as a framework to immobilize tandem repeats of the cyanobacterial metallothionein SmtA from Synechococcus elongatus (strain PCC 7942). SmtA is a low molecular weight cysteine-rich protein known to bind heavy metals. A series of Cry3Aa-SmtA constructs were produced by the fusion of one, three, or six tandem repeats of SmtA to Cry3Aa. Overexpression of these constructs in Bt resulted in the production of pure Cry3Aa-SmtA fusion crystals that exhibited similar size, crystallinity, and morphology to that of native Cry3Aa protein crystals. All three Cry3Aa-SmtA constructs exhibited efficient binding to cadmium and chromium, with the binding capacity correlated with increasing SmtA copy number. These results suggest the potential use of Cry3Aa-SmtA crystals as a novel biodegradable and cost-effective approach to the removal of toxic heavy metals from the environment.
Codex (Cognitive Disorders Examination) Decision Tree Modified for the Detection of Dementia and MCI
Authors: Ziso Besa , Larner Andrew J.
Published: June 2019

Many cognitive screening instruments are available to assess patients with cognitive symptoms in whom a diagnosis of dementia or mild cognitive impairment is being considered. Most are quantitative scales with specified cut-off values. In contrast, the cognitive disorders examination or Codex is a two-step decision tree which incorporates components from the Mini-Mental State Examination (MMSE) (three word recall, spatial orientation) along with a simplified clock drawing test to produce categorical outcomes defining the probability of dementia diagnosis and, by implication, directing clinician response (reassurance, monitoring, further investigation, immediate treatment). Codex has been shown to have high sensitivity and specificity for dementia diagnosis but is less sensitive for the diagnosis of mild cognitive impairment (MCI). We examined minor modifications to the Codex decision tree to try to improve its sensitivity for the diagnosis of MCI, based on data extracted from studies of two other cognitive screening instruments, the Montreal Cognitive Assessment and Free-Cog, which are more stringent than MMSE in their tests of delayed recall. Neither modification proved of diagnostic value for mild cognitive impairment. Possible explanations for this failure are considered.
A Correntropy-Based Proportionate Affine Projection Algorithm for Estimating Sparse Channels with Impulsive Noise
Authors: Jiang Zhengxiong , Li Yingsong , Huang Xinqi
Published: June 2019

A novel robust proportionate affine projection (AP) algorithm is devised for estimating sparse channels, which often occur in network echo and wireless communication channels. The newly proposed algorithm is realized by using the maximum correntropy criterion (MCC) and the data reusing scheme used in AP to overcome the identification performance degradation of the traditional PAP algorithm in impulsive noise environments. The proposed algorithm is referred to as the proportionate affine projection maximum correntropy criterion (PAPMCC) algorithm, which is derived in the context of channel estimation framework. Many simulation results were obtained to verify that the PAPMCC algorithm is superior to early reported AP algorithms with different input signals under impulsive noise environments.
Investigation of Laminar–Turbulent Transition on a Rotating Wind-Turbine Blade of Multimegawatt Class with Thermography and Microphone Array
Authors: Reichstein Torben , Schaffarczyk Alois Peter , Dollinger Christoph , Balaresque Nicolas , Schülein Erich , Jauch Clemens , Fischer Andreas
Published: June 2019

Knowledge about laminar–turbulent transition on operating multi megawatt wind turbine (WT) blades needs sophisticated equipment like hot films or microphone arrays. Contrarily, thermographic pictures can easily be taken from the ground, and temperature differences indicate different states of the boundary layer. Accuracy, however, is still an open question, so that an aerodynamic glove, known from experimental research on airplanes, was used to classify the boundary-layer state of a 2 megawatt WT blade operating in the northern part of Schleswig-Holstein, Germany. State-of-the-art equipment for measuring static surface pressure was used for monitoring lift distribution. To distinguish the laminar and turbulent parts of the boundary layer (suction side only), 48 microphones were applied together with ground-based thermographic cameras from two teams. Additionally, an optical camera mounted on the hub was used to survey vibrations. During start-up (SU) (from 0 to 9 rpm), extended but irregularly shaped regions of a laminar-boundary layer were observed that had the same extension measured both with microphones and thermography. When an approximately constant rotor rotation (9 rpm corresponding to approximately 6 m/s wind speed) was achieved, flow transition was visible at the expected position of 40% chord length on the rotor blade, which was fouled with dense turbulent wedges, and an almost complete turbulent state on the glove was detected. In all observations, quantitative determination of flow-transition positions from thermography and microphones agreed well within their accuracy of less than 1%.
A Comparative Study between Machine Learning Algorithm and Artificial Intelligence Neural Network in Detecting Minor Bearing Fault of Induction Motors
Authors: Esakimuthu Pandarakone Shrinathan , Mizuno Yukio , Nakamura Hisahide
Published: June 2019

Most of the mechanical systems in industries are made to run through induction motors (IM). To maintain the performance of the IM, earlier detection of minor fault and continuous monitoring (CM) are required. Among IM faults, bearing faults are considered as indispensable because of its high probability incidence nature. CM mainly depends upon signal processing and fault detection techniques. In recent decades, various methods have been involved in detecting the bearing fault using machine learning (ML) algorithms. Additionally, the role of artificial intelligence (AI), a growing technology, has also been used in fault diagnosis of IM. Taking the necessity of minor fault detection and the detailed study about the role of ML and AI to detect the bearing fault, the present study is performed. A comprehensive study is conducted by considering various diagnosis methods from ML and AI for detecting a minor bearing fault (hole and scratch). This study helps in understanding the difference between the diagnosis approach and their effectiveness in detecting an IM bearing fault. It is accomplished through FFT (fast Fourier transform) analysis of the load current and the extracted features are used to train the algorithm. The application is extended by comparing the result of ML and AI, and then explaining the specific purpose of use.
A Techno-Economic Study of 100% Renewable Energy for a Residential Household in China
Authors: Lv Zhe , Wang Zengping , Xu Wanyu
Published: June 2019

In the context of global warming and energy shortage, this paper discusses the techno-economic feasibility of a residential household based on 100% renewable energy in China. The energy storage life, equipment’s residual value, system shortage capacity and atmospheric pollution emissions were considered comprehensively. A life cycle evaluation model based on the net present value (NPV) was built. Taking a real household as an example, the levelised cost of energy (LCOE) is 0.146 $/kW and the unmet load is only 0.86%, which has a big economic advantage when compared with diesel generators. If grid-connected, the system can bring $8079 in 25 years when the LCOE is −0.062 $/kW. The effects of the allowed shortage capacity, renewable energy resources, battery price and the allowed depth of discharge on the economy and energy structure were examined. For example, due to the features of the residential load, the influence of wind resource richness is more obvious than the irradiance. The maximum depth of discharge has less impact on the economy. This paper verifies the techno-economic rationality and feasibility of 100% renewable energy for a household.
A Synchronous Sampling Based Harmonic Analysis Strategy for Marine Current Turbine Monitoring System under Strong Interference Conditions
Authors: Zhang Milu , Wang Tianzhen , Tang Tianhao , Liu Zhuo , Claramunt Christophe
Published: June 2019

Affected by high density, non-uniform, and unstructured seawater environment, fault detection of Marine Current Turbine (MCT) faces various fault features and strong interferences. To solve these problems, a harmonic analysis strategy based on zero-crossing estimation and Empirical Mode Decomposition (EMD) filter banks is proposed. First, the detection problems of rotor imbalance fault under strong interference conditions are described through an analysis of the fault mechanism and operation environment of MCT. Therefore, against various fault features, a zero-crossing estimation is proposed to calculate instantaneous frequency. Last, and in order to solve the problem that the frequency and amplitude of the operating parameters are partially or completely covered by interference, a band-pass filter based on EMD is used, together with a characteristic frequency selected by a Pearson correlation coefficient. This strategy can accurately detect the multiplicative faults under strong interference conditions, and can be applied to the MCT fault detection system. Theoretical and experimental results verify the effectiveness of the proposed strategy.
Research on Optimization Allocation Scheme of Initial Carbon Emission Quota from the Perspective of Welfare Effect
Authors: Wu Qunli , Zhang Hongjie
Published: June 2019

The initial allocation of carbon emission quotas should be of primary concern when establishing China’s unified carbon emission trading market. Essentially, the issue of national carbon quota allocation is an allocation among China’s provinces. The novel bi-level allocation scheme that is based on weighted voting model is put forward, which divides allocation process into two levels, given that there are great regional differences in China. At the first level, k-means clustering is employed to cluster 29 provinces into four categories that are based on emission abatement responsibility, potential, capacity, pressure, and motivation. Subsequently, the national carbon quotas are allocated to the four classes. At the second level, carbon quotas of a class are allocated to each region in this class. The weighted voting models are constructed for the two levels, where each region selects their preferable scheme from three fundamental allocation schemes that are based on their voting rights. The comprehensive index method quantifies each region’s voting rights, which utilizes the information entropy method at the first level and the analytic hierarchy process (AHP) at the second level. The carbon trading market is simulated and welfare effects obtained from carbon trading market under different allocation schemes are measured to verify the rationality of the proposed model. The results indicate: (1) the emission abatement burdens are borne by all provinces in China, but the burden shares are different, which are related to their respective carbon emission characteristics. (2) The differences in carbon intensity among regions in 2030 have narrowed on the basis of the results of 2005, which means that the proposed scheme can balance corresponding differences. (3) When compared with three fundamental allocation schemes, the bi-level allocation scheme can obtain the most welfare effects, while the differences in the welfare effect among regions under this scheme are the smallest, which indicates that the proposed model is feasible for policy-maker.
Modeling an Alternate Operational Ground Source Heat Pump for Combined Space Heating and Domestic Hot Water Power Sizing
Authors: Ahmed Kaiser , Fadejev Jevgeni , Kurnitski Jarek
Published: June 2019

This study developed an alternate operational control system for ground source heat pumps (GSHP), which was applied to determine combined space heating and domestic hot water (DHW) power equations at design temperature. A domestic GSHP with an alternate control system was implemented in a whole building simulation model following the heat deficiency for space heating based on degree minute counting. A simulated GSHP system with 200 L storage tank resulted in 13%–26% power reduction compared to the calculation of the same system with existing European standards, which required separate space heating and DHW power calculation. The periodic operation utilized the thermal mass of the building with the same effect in the case of light and heavy-weight building because of the very short cycle of 30 min. Room temperatures dropped during the DHW heating cycle but kept within comfort range. The developed equations predict the total power as a function of occupancy, peak and average DHW consumption with variations of 0%–2.2% compared to the simulated results. DHW heating added the total power in modern low energy buildings by 21%–41% and 13%–26% at design temperatures of −15 °C and −26 °C, respectively. Internal heat gains reduced the power so that the reduction effect compensated the effect of DHW heating in the case of a house occupied by three people. The equations could be used for power sizing of any heat pump types, which has alternate operation principle and hydronic heating system.
Electric Vehicle Charging Process and Parking Guidance App
Authors: Alface Gonçalo , Ferreira João C. , Pereira Rúben
Published: June 2019

This research work presents an information system to handle the problem of real-time guidance towards free charging slot in a city using past date and prediction and collaborative algorithms since there is no real-time system available to provide information if a charging spot is free or occupied. We explore the prediction approach using past data correlated with weather conditions. This approach will help the driver in the daily use of his electric vehicle, minimizing the problem of range anxiety, provide guidance towards charging spots with a probability value of being available for charging in a context for the app and smart cities. This work handles the uncertainty of the drivers to get a suitable and vacant place at a charging station because missing real-time information from the system and also during the driving process towards the free charging spot can be taken. We introduce a framework to allow collaboration and prediction process using past related data.
The Use of Sentinel-3 Imagery to Monitor Cyanobacterial Blooms
Authors: Ogashawara Igor
Published: June 2019

Cyanobacterial harmful algal blooms (CHABs) have been a concern for aquatic systems, especially those used for water supply and recreation. Thus, the monitoring of CHABs is essential for the establishment of water governance policies. Recently, remote sensing has been used as a tool to monitor CHABs worldwide. Remote monitoring of CHABs relies on the optical properties of pigments, especially the phycocyanin (PC) and chlorophyll-a (chl-a). The goal of this study is to evaluate the potential of recent launch the Ocean and Land Color Instrument (OLCI) on-board the Sentinel-3 satellite to identify PC and chl-a. To do this, OLCI images were collected over the Western part of Lake Erie (U.S.A.) during the summer of 2016, 2017, and 2018. When comparing the use of traditional remote sensing algorithms to estimate PC and chl-a, none was able to accurately estimate both pigments. However, when single and band ratios were used to estimate these pigments, stronger correlations were found. These results indicate that spectral band selection should be re-evaluated for the development of new algorithms for OLCI images. Overall, Sentinel 3/OLCI has the potential to be used to identify PC and chl-a. However, algorithm development is needed.
Economics of Early Intervention to Suppress a Potential Spruce Budworm Outbreak on Crown Land in New Brunswick, Canada
Authors: Liu Eric Ye , Lantz Van A. , MacLean David A. , Hennigar Chris
Published: June 2019

We investigated the potential economic impacts of future spruce budworm (Choristoneura fumiferana Clem.) (SBW) outbreaks on 2.8 million ha of Crown land in New Brunswick, Canada and compared an early intervention strategy (EIS) with foliage protection approaches. We coupled the Spruce Budworm Decision Support System (SBW DSS) with a Computable General Equilibrium (CGE) model to assess the impacts of EIS and foliage protection on 0%, 5%, 10%, and 20% of susceptible Crown (publicly owned) forest, under moderate and severe SBW outbreak scenarios. Cumulative available harvest supply from 2017 to 2067 was projected to be reduced by 29 to 43 million m3, depending upon SBW outbreak severity, and a successful EIS approach would prevent this loss. These harvest reductions were projected to reduce total economic output by $25 billion (CAD) to $35 billion. Scenarios using biological insecticide foliage protection over 20% of susceptible Crown forest area were projected to reduce losses to 6–17 million m3 and $0.5–4.1 billion. Depending upon SBW outbreak severity, EIS was projected to have benefit/cost ratios of 3.8 to 6.4 and net present values of $186 million to $353 million, both higher than foliage protection strategies. Sensitivity analysis scenarios of ‘what if’ EIS partially works (80% or 90%) showed that these produced superior timber harvest savings than the best foliage protection scenario under severe SBW outbreak conditions and generally superior results under moderate outbreak scenarios. Overall, results support the continued use of EIS as the preferred strategy on economic grounds to protect against SBW outbreaks on Crown land in New Brunswick.
On the Kutta Condition in Compressible Flow over Isolated Airfoils
Authors: Mohebbi Farzad , Evans Ben , Sellier Mathieu
Published: June 2019

This paper presents a novel and accurate method to implement the Kutta condition in solving subsonic (subcritical) inviscid isentropic compressible flow over isolated airfoils using the stream function equation. The proposed method relies on body-fitted grid generation and solving the stream function equation for compressible flows in computational domain using finite-difference method. An expression is derived for implementing the Kutta condition for the airfoils with both finite angles and cusped trailing edges. A comparison of the results obtained from the proposed numerical method and the results from experimental and other numerical methods reveals that they are in excellent agreement, which confirms the accuracy and correctness of the proposed method.
Teaching Fluid Mechanics and Thermodynamics Simultaneously through Pipeline Flow Experiments
Authors: Pal Rajinder
Published: June 2019

Entropy and entropy generation are abstract and illusive concepts for undergraduate students. In general, students find it difficult to visualize entropy generation in real (irreversible) processes, especially at a mechanistic level. Fluid mechanics laboratory can assist students in making the concepts of entropy and entropy generation more tangible. In flow of real fluids, dissipation of mechanical energy takes place due to friction in fluids. The dissipation of mechanical energy in pipeline flow is reflected in loss of pressure of fluid. The degradation of high quality mechanical energy into low quality frictional heat (internal energy) is simultaneously reflected in the generation of entropy. Thus, experiments involving measurements of pressure gradient as a function of flow rate in pipes offer an opportunity for students to visualize and quantify entropy generation in real processes. In this article, the background in fluid mechanics and thermodynamics relevant to the concepts of mechanical energy dissipation, entropy and entropy generation are reviewed briefly. The link between entropy generation and mechanical energy dissipation in pipe flow experiments is demonstrated both theoretically and experimentally. The rate of entropy generation in pipeline flow of Newtonian fluids is quantified through measurements of pressure gradient as a function of flow rate for a number of test fluids. The factors affecting the rate of entropy generation in pipeline flows are discussed.
A Modification Fractional Homotopy Perturbation Method for Solving Helmholtz and Coupled Helmholtz Equations on Cantor Sets
Authors: Baleanu Dumitru , Jassim Hassan Kamil
Published: June 2019

In this paper, we apply a new technique, namely, the local fractional Laplace homotopy perturbation method (LFLHPM), on Helmholtz and coupled Helmholtz equations to obtain analytical approximate solutions. The iteration procedure is based on local fractional derivative operators (LFDOs). This method is a combination of the local fractional Laplace transform (LFLT) and the homotopy perturbation method (HPM). The method in general is easy to implement and yields good results. Illustrative examples are included to demonstrate the validity and applicability of the new technique.
NetR and AttR, Two New Bioinformatic Tools to Integrate Diverse Datasets into Cytoscape Network and Attribute Files
Authors: Halajyan Armen , Weingart Natalie , Yeahia Mirza , Loza-Coll Mariano
Published: June 2019

High-throughput technologies have allowed researchers to obtain genome-wide data from a wide array of experimental model systems. Unfortunately, however, new data generation tends to significantly outpace data re-utilization, and most high throughput datasets are only rarely used in subsequent studies or to generate new hypotheses to be tested experimentally. The reasons behind such data underutilization include a widespread lack of programming expertise among experimentalist biologists to carry out the necessary file reformatting that is often necessary to integrate published data from disparate sources. We have developed two programs (NetR and AttR), which allow experimental biologists with little to no programming background to integrate publicly available datasets into files that can be later visualized with Cytoscape to display hypothetical networks that result from combining individual datasets, as well as a series of published attributes related to the genes or proteins in the network. NetR also allows users to import protein and genetic interaction data from InterMine, which can further enrich a network model based on curated information. We expect that NetR/AttR will allow experimental biologists to mine a largely unexploited wealth of data in their fields and facilitate their integration into hypothetical models to be tested experimentally.
Anaerobically-Digested Brewery Wastewater as a Nutrient Solution for Substrate-Based Food Production
Authors: Riera-Vila Ignasi , Anderson Neil O. , Flavin Hodge Claire , Rogers Mary
Published: June 2019

Urban agriculture, due to its location, can play a key role in recycling urban waste streams, promoting nutrient recycling, and increasing sustainability of food systems. This research investigated the integration of brewery wastewater treatment through anaerobic digestion with substrate-based soilless agriculture. An experiment was conducted to study the performance of three different crops (mustard greens (Brassica juncea), basil (Ocimum basilicum), and lettuce (Lactuca sativa) grown with digested and raw brewery wastewater as fertilizer treatments. Mustard greens and lettuce grown in digested wastewater produced similar yields as the inorganic fertilizer control treatment, while basil had slightly lower yields. In all cases, crops in the digested wastewater treatments produced higher yields than raw wastewater or the no fertilizer control, indicating that nutrients in the brewery wastewater can be recovered for food production and diverted from typical urban waste treatment facilities.
Simulated Abiotic Injury Alters Yields of Southern Interspecific Hybrid Grape Cultivars
Authors: Stafne Eric T. , Carroll Becky L.
Published: June 2019

Climate change, including more volatile weather and longer growing seasons, is causing stress on grapevines (Vitis spp.). A change in harvest timing of wine grapes can have significant consequences. Thus, two methods (crop forcing and complete removal of green tissue) were employed to simulate abiotic vine injury. The harvest of bunch grapes in Mississippi occurs during July, a very hot month. ‘Miss Blanc’ and ‘Villard Blanc’ had four different crop forcing treatments imposed to determine yield amount and harvest timing. All treatments reduced yield. Harvest was delayed by 50 days, a potentially positive shift that was not enough to escape high temperatures. ‘Villard Blanc’ had no flower or fruit development after crop forcing treatments in May and June. ‘Miss Blanc’ yields were also significantly reduced by these treatments. Removal of green tissue to simulate injury from weather events such as frost, freeze, wind, or hail in both Mississippi and Oklahoma revealed that lost growth could reduce yields from 19% to 81%, which could influence grape grower management decisions.
Development and Implementation of an Internet Survey to Assess Community Health in the Face of a Health Crisis: Data from the Pregnancy and Birth Survey of the Fukushima Health Management Survey, 2016
Authors: Nakano Hironori , Ishii Kayoko , Goto Aya , Yasumura Seiji , Ohira Tetsuya , Fujimori Keiya
Published: June 2019

The Pregnancy and Birth Survey of the Fukushima Health Management Survey is a questionnaire survey that has been conducted annually since 2011 in Fukushima Prefecture. Since 2016, the survey has been available online as well as in paper form. This study aimed to determine whether making the survey available online improved response rates and to identify the characteristics of paper and online survey respondents and their results. Using LimeSurvey, we constructed an online survey environment that enabled responses via computer or mobile device. Respondents could choose whether to respond on paper or online. The response rate for the 2016 survey was 51.8%, an increase of 3.5% over the previous year. Of these responses, 15.8% were made online. Online respondents were mostly primiparous. Further, while there was no difference in the percentage of respondents who provided free responses, the amount written was higher in paper surveys than in online surveys. The combination of paper and online surveys increased convenience for respondents and contributed to improved response rates. In addition, paper surveys were superior in terms of allowing respondents to express their feelings and opinions.
Comparison of Self-Administered Web-Based and Interviewer Printed Food Frequency Questionnaires for Dietary Assessment in Italian Adolescents
Authors: Barchitta Martina , Maugeri Andrea , Agrifoglio Ottavia , Favara Giuliana , La Mastra Claudia , La Rosa Maria Clara , Magnano San Lio Roberta , Agodi Antonella
Published: June 2019

Innovative tools for assessing food and nutrient intakes in adolescence are essential to uncover the long-term effects of diet on chronic diseases. Here, we developed and compared a web-based self-administered food frequency questionnaire (web-FFQ) with a traditional interviewer printed FFQ (print-FFQ) among 174 Italian adolescents (aged 15–18 years). To assess the reliability of the web-FFQ compared with the print-FFQ, we used Spearman’s rank correlation coefficients, Wilcoxon rank test, quartile misclassification analysis, Cohen’s weighted kappa and the Bland–Altman method. Correlation coefficients ranged from 0.14 (i.e., pizza) to 0.67 (i.e., raw vegetables) for foods, and from 0.45 (i.e., monounsaturated fatty acids, MUFA) to 0.62 (i.e., zinc) for nutrients. Results from the Wilcoxon rank test showed that food and nutrient intakes were comparable between two FFQs, except for nuts, shellfish, fruit juices and MUFA. Adolescents classified into the same or adjacent quartiles ranged from 68.2% (i.e., tea) to 89.1% (i.e., raw vegetables and dipping sauces) for foods, and from 77.2% (i.e., vitamin C) to 87.2% (i.e., folate and calcium) for nutrients. Except for pizza, the weighted kappa indicated moderate to substantial agreement for other foods and nutrients. Finally, we demonstrated that the web-FFQ significantly overestimated shellfish and fruit juice intakes, while it underestimated nuts, canned fish, olive oil, total energy intake, fatty acids and calcium. The limits of agreement analysis indicated moderate to wide individual differences for all groups. In conclusion, our self-administered web-FFQ represents an easy, suitable and cost-effective tool for assessing food and nutrient intakes in adolescents. However, the wide individual differences in level of agreement suggest that additional refinements and calibrations are necessary to investigate the effects of absolute nutrient intakes at the individual level.
Housing Design and Community Care: How Home Modifications Reduce Care Needs of Older People and People with Disability
Authors: Carnemolla Phillippa , Bridge Catherine
Published: June 2019

The extent to which housing design can minimise levels of community caregiving has remained largely unmeasured. This paper reports the potential for home modifications to reduce caregiving in the peoples’ homes, particularly older people and people with a disability. It contributes to new knowledge in understanding how housing can play a role in community caregiving and acknowledges the role of the built environment in managing care levels in ageing societies. This paper analyses self-reported care data from 157 Australian community care recipients (average age: 72 years) who had received home modifications within the past 6 months. A before/after comparison of care provided revealed that home modifications reduced hours of care provided by 42% per week. More detailed analysis revealed that the positive association of home modifications with care reduction is stronger with informal care (46% reduction) followed by formal care (16% reduction). These results suggest the role that home modifications, and housing design in general, play in reducing care needs in a community setting.
Impact of Urea Addition and Rhizobium Inoculation on Plant Resistance in Metal Contaminated Soil
Authors: Shen Guoting , Ju Wenliang , Liu Yuqing , Guo Xiaobin , Zhao Wei , Fang Linchuan
Published: June 2019

Legume-rhizobium symbiosis has been heavily investigated for their potential to enhance plant metal resistance in contaminated soil. However, the extent to which plant resistance is associated with the nitrogen (N) supply in symbiont is still uncertain. This study investigates the effect of urea or/and rhizobium (Sinorhizobium meliloti) application on the growth of Medicago sativa and resistance in metals contaminated soil (mainly with Cu). The results show that Cu uptake in plant shoots increased by 41.7%, 69%, and 89.3% with urea treatment, rhizobium inoculation, and their combined treatment, respectively, compared to the control group level. In plant roots, the corresponding values were 1.9-, 1.7-, and 1.5-fold higher than the control group values, respectively. Statistical analysis identified that N content was the dominant variable contributing to Cu uptake in plants. Additionally, a negative correlation was observed between plant oxidative stress and N content, indicating that N plays a key role in plant resistance. Oxidative damage decreased after rhizobium inoculation as the activities of antioxidant enzymes (catalase and superoxide dismutase in roots and peroxidase in plant shoots) were stimulated, enhancing plant resistance and promoting plant growth. Our results suggest that individual rhizobium inoculation, without urea treatment, is the most recommended approach for effective phytoremediation of contaminated land.
Illicit Cigarette Consumption and Government Revenue Loss in Vietnam: Evidence from a Primary Data Approach
Authors: Nguyen Minh T. , Dao Son The , Nguyen Nga Que , Bowling Mike , Ross Hana , So Anthony D.
Published: June 2019

This article provides the first comprehensive picture and independent estimates of both illicit cigarette consumption and the resulting government tax revenue loss in Vietnam using data from a representative survey of cigarette smokers in 12 Vietnamese provinces. The survey consisted of face-to-face interviews and on-site cigarette pack examinations. We find that more than 720 million illicit cigarette packs, or 20.7% of total cigarette consumption, circulated in Vietnam in 2012. Consequently, government tax revenue loss due to illicit trade ranged from US $223 to 295 million. Our estimates also indicate that (1) the most popular illicit brands were Jet and Hero, both were sold at higher prices than the average legal brand; (2) the average price of illicit cigarettes was 51% higher than the average price of legal cigarettes; and (3) majority of illicit cigarettes were sold at convenience stores, which were registered and licensed businesses. Our findings suggest that prices are not a driver of illicit cigarette consumption in Vietnam, and this illicit trade is at least partially a consequence of weak market control enforcement.
Media Access is Associated with Knowledge of Optimal Water, Sanitation and Hygiene Practices in Tanzania
Authors: Alexander Chelsi C. , Shrestha Shilpa , Tounkara Mamadou D. , Cooper Shelly , Hunt Leiema , Hoj Taylor H. , Dearden Kirk , Kezakubi Dotto , Atugonza Vianney , West Joshua , Crookston Benjamin , Hall Cougar
Published: June 2019

The importance of water, sanitation and hygiene (WASH) behaviors in low- and middle-income countries in preventing childhood illness is well established. Tanzania is known to have high rates of chronic malnutrition and childhood stunting—both of which have been linked to poor WASH practices. Interviews were conducted with 5000 primary caregivers of children aged 0–23 months. Four composite WASH knowledge variables were created to assess the relationship between WASH knowledge and access to different forms of media, such as television, radio, and mobile phones. WASH knowledge variables measure knowledge of when to wash hands, the need for soap when washing hands, when to wash a baby’s hands, and how eating soil or chicken feces can affect a baby’s health. Logistic and linear regression analyses were conducted to measure the association between media access and WASH knowledge. Having watched television was positively associated with higher WASH knowledge indicators (all p < 0.05). Higher WASH knowledge was positively associated with more frequent handwashing after cleaning a baby’s bottom (all p < 0.0001). The quantity of media access also had a positive linear effect on handwashing; more media items owned was associated with increases in handwashing. Study findings indicate media access is associated with WASH knowledge among caregivers in resource-poor settings.
Acute Toxicity of Divalent Mercury Ion to Anguilla japonica from Seawater and Freshwater Aquaculture and Its Effects on Tissue Structure
Authors: Tang Yuanqiang , Liu Yunguo , Zhang Tao , Li Jiang , Wang Xiaohua , Zhang Wei , Zeng Guangming , Liu Shaobo , Guan Lei
Published: June 2019

The acute toxicity of divalent mercury ion to Anguilla japonica from seawater and freshwater aquaculture was assessed. In particular, the effects of toxicity on the microstructures of the gill and liver tissues were examined using the hydrostatic method, without feeding, at a water temperature of 20 °C. The median lethal concentrations (LC50) of divalent mercury ion to fishes in seawater and freshwater over various durations were: 24 h = 1.637 and 1.428 mg/L; 48 h = 1.562 and 1.377 mg/L; 72 h = 1.530 and 1.284 mg/L; and 96 h = 1.442 and 1.228 mg/L. The safety mass concentrations were 0.1442 and 0.01228 mg/L, respectively. After exposure to divalent mercury ion, adhesion between the gill lamellae and massive cellular disintegration and necrotic shedding were observed in the gill tissue sections. The liver tissues underwent hyperemia and swelling, with the appearance of blood spots, swelling of the hepatocyte mitochondria, dilation of the rough endoplasmic reticulum, and intercellular inflation.
Association between Occupational and Radiological Factors and Nontuberculous Mycobacteria Lung Infection in Workers with Prior Dust Exposure
Authors: Lee Ji-Won , Myong Jun-Pyo
Published: June 2019

This retrospective cross-sectional study was conducted to identify the factors that promote the risk of nontuberculous mycobacteria (NTM) lung infection in subjects with prior occupational dust exposure. All consecutive patients with a history of occupational dust exposure whose expectorated sputum, bronchial wash, or bronchial lavage was subjected to acid-fast Bacilli culture in a tertiary hospital between 2011 and 2016 were identified. The patients who were infected with NTM were identified according to the bacteriological criteria of the American Thoracic Society (ATS) and The Infectious Diseases Society of America (IDSA) statement. Pneumoconiosis-associated radiological findings were graded according to the International Labor Organization guidelines. Of the 1392 patients with prior dust exposure, NTM was isolated from 82. Logistic regression analysis showed that risk factors for NTM lung infection were a history of pulmonary tuberculosis (adjusted odds ratio [aOR] = 1.82, 95% confidence intervals [CI] = 1.03–3.16). Moreover, the unadjusted odds ratios (ORs) were higher when both small-opacity profusion and the large-opacity grades increased. Even after adjustment, the ORs for the A, B, and C large-opacity grades were 2.32 (95% CI = 1.01–4.99), 2.68 (95% CI = 1.35–5.24), and 7.58 (95% CI = 3.02–17.95). Previous tuberculosis, bronchiectasis, and especially extensive small-opacity profusion, and high large-opacity grade associated significantly with NTM lung infection in dust-exposed workers.
ERK Pathway in Activated, Myofibroblast-Like, Hepatic Stellate Cells: A Critical Signaling Crossroad Sustaining Liver Fibrosis
Authors: Foglia Beatrice , Cannito Stefania , Bocca Claudia , Parola Maurizio , Novo Erica
Published: June 2019

Fibrogenic progression of chronic liver disease, whatever the etiology, is characterized by persistent chronic parenchymal injury, chronic activation of inflammatory response, and sustained activation of liver fibrogenesis, and of pathological wound healing response. A critical role in liver fibrogenesis is played by hepatic myofibroblasts (MFs), a heterogeneous population of α smooth-muscle actin—positive cells that originate from various precursor cells through a process of activation and transdifferentiation. In this review, we focus the attention on the role of extracellular signal-regulated kinase (ERK) signaling pathway as a critical one in modulating selected profibrogenic phenotypic responses operated by liver MFs. We will also analyze major therapeutic antifibrotic strategies developed in the last two decades in preclinical studies, some translated to clinical conditions, designed to interfere directly or indirectly with the Ras/Raf/MEK/ERK signaling pathway in activated hepatic MFs, but that also significantly increased our knowledge on the biology and pathobiology of these fascinating profibrogenic cells.
Chemotaxis Towards Aromatic Compounds: Insights from Comamonas testosteroni
Authors: Wang Yun-Hao , Huang Zhou , Liu Shuang-Jiang
Published: June 2019

Chemotaxis is an important physiological adaptation that allows many motile bacteria to orientate themselves for better niche adaptation. Chemotaxis is best understood in Escherichia coli. Other representative bacteria, such as Rhodobacter sphaeroides, Pseudomonas species, Helicobacter pylori, and Bacillus subtilis, also have been deeply studied and systemically summarized. These bacteria belong to α-, γ-, ε-Proteobacteria, or Firmicutes. However, β-Proteobacteria, of which many members have been identified as holding chemotactic pathways, lack a summary of chemotaxis. Comamonas testosteroni, belonging to β-Proteobacteria, grows with and chemotactically responds to a range of aromatic compounds. This paper summarizes the latest research on chemotaxis towards aromatic compounds, mainly from investigations of C. testosteroni and other Comamonas species.
Proliferative Pathways of Vascular Smooth Muscle Cells in Response to Intermittent Hypoxia
Authors: Kyotani Yoji , Takasawa Shin , Yoshizumi Masanori
Published: June 2019

Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia (IH) and is a risk factor for cardiovascular diseases (e.g., atherosclerosis) and chronic inflammatory diseases (CID). The excessive proliferation of vascular smooth muscle cells (VSMCs) plays a pivotal role in the progression of atherosclerosis. Hypoxia-inducible factor-1 and nuclear factor-κB are thought to be the main factors involved in responses to IH and in regulating adaptations or inflammation pathways, however, further evidence is needed to demonstrate the underlying mechanisms of this process in VSMCs. Furthermore, few studies of IH have examined smooth muscle cell responses. Our previous studies demonstrated that increased interleukin (IL)-6, epidermal growth factor family ligands, and erbB2 receptor, some of which amplify inflammation and, consequently, induce CID, were induced by IH and were involved in the proliferation of VSMCs. Since IH increased IL-6 and epiregulin expression in VSMCs, the same phenomenon may also occur in other smooth muscle cells, and, consequently, may be related to the incidence or progression of several diseases. In the present review, we describe how IH can induce the excessive proliferation of VSMCs and we develop the suggestion that other CID may be related to the effects of IH on other smooth muscle cells.
Enhanced Protection of Biological Membranes during Lipid Peroxidation: Study of the Interactions between Flavonoid Loaded Mesoporous Silica Nanoparticles and Model Cell Membranes
Authors: Mandić Lucija , Sadžak Anja , Strasser Vida , Baranović Goran , Domazet Jurašin Darija , Dutour Sikirić Maja , Šegota Suzana
Published: June 2019

Flavonoids, polyphenols with anti-oxidative activity have high potential as novel therapeutics for neurodegenerative disease, but their applicability is rendered by their poor water solubility and chemical instability under physiological conditions. In this study, this is overcome by delivering flavonoids to model cell membranes (unsaturated DOPC) using prepared and characterized biodegradable mesoporous silica nanoparticles, MSNs. Quercetin, myricetin and myricitrin have been investigated in order to determine the relationship between flavonoid structure and protective activity towards oxidative stress, i.e., lipid peroxidation induced by the addition of hydrogen peroxide and/or Cu2+ ions. Among investigated flavonoids, quercetin showed the most enhanced and prolonged protective anti-oxidative activity. The nanomechanical (Young modulus) measurement of the MSNs treated DOPC membranes during lipid peroxidation confirmed attenuated membrane damage. By applying a combination of experimental techniques (atomic force microscopy—AFM, force spectroscopy, electrophoretic light scattering—ES and dynamic light scattering—DLS), this work generated detailed knowledge about the effects of flavonoid loaded MSNs on the elasticity of model membranes, especially under oxidative stress conditions. Results from this study will pave the way towards the development of innovative and improved markers for oxidative stress-associated neurological disorders. In addition, the obtained could be extended to designing effective delivery systems of other high potential bioactive molecules with an aim to improve human health in general.