bluuebunny/crossref_metadata_2025 huggingface dataset:
Barriers and facilitators for the sustainability of digital health interventions in low and middle-income countries: A systematic review
Authors: Kaboré Soutongnoma Safiata , Ngangue Patrice , Soubeiga Dieudonné , Barro Abibata , Pilabré Arzouma Hermann , Bationo Nestor , Pafadnam Yacouba , Drabo Koiné Maxime , Hien Hervé , Savadogo Gueswendé Blaise Léon
Published: November 2022

Background

Digital health interventions (DHIs) have increased exponentially all over the world. Furthermore, the interest in the sustainability of digital health interventions is growing significantly. However, a systematic synthesis of digital health intervention sustainability challenges is lacking. This systematic review aimed to identify the barriers and facilitators for the sustainability of digital health intervention in low and middle-income countries.

Methods

Three electronic databases (PubMed, Embase and Web of Science) were searched. Two independent reviewers selected eligible publications based on inclusion and exclusion criteria. Data were extracted and quality assessed by four team members. Qualitative, quantitative or mixed studies conducted in low and middle-income countries and published from January 2000 to May 2022 were included.

Results

The sustainability of digital health interventions is very complex and multidimensional. Successful sustainability of digital health interventions depends on interdependent complex factors that influence the implementation and scale-up level in the short, middle and long term. Barriers identified among others are associated with infrastructure, equipment, internet, electricity and the DHIs. As for the facilitators, they are more focused on the strong commitment and involvement of relevant stakeholders: Government, institutional, sectoral, stakeholders' support, collaborative networks with implementing partners, improved satisfaction, convenience, privacy, confidentiality and trust in clients, experience and confidence in using the system, motivation and competence of staff. All stakeholders play an essential role in the process of sustainability. Digital technology can have long term impacts on health workers, patients, and the health system, by improving data management for decision-making, the standard of healthcare service delivery and boosting attendance at health facilities and using services. Therefore, management changes with effective monitoring and evaluation before, during, and after DHIs are essential.

Conclusion

The sustainability of digital health interventions is crucial to maintain good quality healthcare, especially in low and middle-income countries. Considering potential barriers and facilitators for the sustainability of digital health interventions should inform all stakeholders, from their planning until their scaling up. Besides, it would be appropriate at the health facilities level to consolidate facilitators and efficiently manage barriers with the participation of all stakeholders.
Propagation of 2-D SH waves in random media: Insights from ab initio numerical simulations and transport theory
Authors: Celorio Malcon , Chaljub Emmanuel , Margerin Ludovic , Stehly Laurent
Published: November 2022

Increasing the frequency range of physics-based predictions of earthquake ground motions requires to account for small-scale heterogeneities, which can only be described in a stochastic way. Although many studies have addressed the impact of random heterogeneities on ground motion intensity parameters obtained by numerical simulation, very few have verified the accuracy of their numerical solutions or controlled the scattering regime they were simulating. Here we present a comprehensive analysis ofSHwave propagation in 2D random media which covers a broad range of propagation regimes from ballistic to diffusive. The coherent and incoherent parts of the wavefield are examined independently. The random media consist in correlated density and velocity fluctuations described by a von Kármán autocorrelation function with a Hurst coefficient of 0.25 and a correlation lengtha= 500 m. The Birch correlation coefficient which relates density to velocity fluctuations takes 4 possible values between 0.5 and 1, and the standard deviation of the perturbations is either 5% or 10%. Spectral element simulations of SH wave propagation excited by a plane wave are performed for normalized wavenumbers (ka) up to 5. Analysis of the decay of the coherent wave amplitude, obtained through different averaging procedures, allows for a direct measure of the scattering attenuation, which we successfully compare with the predictions of the Dyson mean field theory. We also present the comparison between the energy envelopes measured from the synthetics and their theoretical counterpart provided by the radiative transfer theory and the diffusion approximation. Excellent agreement is found between numerical simulations and theoretical predictions of radiative transfer theory for the mean intensity. The numerical study highlights the difference of attenuation length between the mean field and the mean intensity. In the forward scattering regime, the peak intensity appears to decay exponentially over a length scale known as the transport mean free path. Furthermore, the fluctuations of intensity in the ballistic peak exhibit a transition from Log-normal to Exponential statistics. This transition occurs for a propagation distance of the order of the mean free path, which offers an alternative method of estimating this parameter.
Potential challenges and future implications for trauma-informed approaches in schools
Authors: Phung Binh
Published: November 2022

Adverse childhood experiences (ACEs) refer to a collection of traumatic life events that can derail future development, impact physical/mental health, and affect learning/behavior into adulthood. In the United States, it is estimated that two-thirds of children and teens will experience traumatic ACEs by the age of 16. Schools can represent one domain in a child’s life where trauma-informed approaches (TIAs) could be helpful to curtail effects of ACEs. This systematic review aimed to identify some positive commonalities/facilitators (e.g., drivers of change) among existing TIAs implemented in US schools. The secondary objectives aimed to evaluate the confidence of qualitative data using GRADE-CERQual and to highlight potential challenges/barriers associated with the implementation process of TIAs in schools. This manuscript presents a thorough overview of the current state of trauma-informed practices in US schools and concludes with a forward-thinking discussion on key implications for enhancing the field of education.
The hidden (overlooked) impact of living through a pandemic: How parents of children with disabilities fared during COVID-19?
Authors: Rakap Salih , Vural-Batik Meryem , Kalkan Sinan , Karnas Mustafa , Sari Halil Ibrahim , Bayrakdar Uygar
Published: November 2022

Objective

The purpose of this study was to investigate the impact of the COVID-19 pandemic on the quality of life of families of children with disabilities and the mediating roles of perceived social support, perceived parental self-efficacy, psychological health, psychological resilience, and perceived family burden on this relationship.

Background

Children with disabilities and their families are among the most affected populations from the restrictions imposed to prevent the spread of COVID-19 virus, as the special education and rehabilitation services they had been receiving were interrupted.

Method

A correlational survey design was used to collect data from 824 parents of children with disabilities in Turkey. Path analysis was used to examine the direct and indirect effects of COVID-19 on family quality of life.

Results

Results indicated that COVID-19 had a significant total effect on family quality of life and perceived social support, parental self-efficacy and psychological resilience had a mediating role on the relationship between impact of the COVID-19 pandemic and family quality of life. Perceived social support was found to the strongest mediator of the relationship between the impact of COVID-19 and the family quality of life, while perceived family burden did not have a statistically significant association with these variables.

Conclusion

Family quality of life for children with disabilities decreased as the impact of COVID-19 pandemic increased. Perceived social support, parental self-efficacy and psychological resilience had mediating roles in the relationship between the impact of the COVID-19 pandemic and family quality of life.

Implications

COVID-19 has resulted in disruption of special education and rehabilitation services for children with disabilities and their families. Changes in daily routines have brought up additional responsibilities to parents of children with disabilities. These additional responsibilities may adversely affect and increase the burden and stress families of children with disabilities experience. The high levels of stress in families negatively affect the welfare and quality of life and result in decreased parental attention to support their children. Findings of this study show the mediating link of perceived social support, parental self-efficacy and psychological resilience in the relationship between the impact of the COVID-19 pandemic and family quality of life. Policymakers and service providers should develop and implement specific care actions to support children with disabilities and their families during and after the pandemic.
Inferring causal effects of homocysteine and B-vitamin concentrations on bone mineral density and fractures: Mendelian randomization analyses
Authors: Fu Liwan , Wang Yuquan , Hu Yue-Qing
Published: November 2022

Objectives

In the progress of bone metabolism, homocysteine (Hcy) and B vitamins play substantial roles. However, the causal associations of homocysteine, B-vitamin concentrations with bone mineral density (BMD), and fractures remain unclear. Therefore, we employed a two-sample Mendelian randomization (MR) design to infer the causal effects of Hcy and B vitamins on BMD and fractures.

Methods

We selected instrumental variables from large genome-wide association studies (GWASs). Specifically, the exposures mainly included Hcy (sample size: 44,147), vitamin B12 (sample size: 45,576), folate (sample size: 37,465), and vitamin B6 (sample size: 1,864). The outcome variables included total body BMD (sample size: 66,628), heel BMD (sample size: 142,487), femoral neck BMD (sample size: 32,735), lumbar spine BMD (sample size: 28,498), and forearm BMD (sample size: 8143). Additionally, the total body BMD in several age strata was also included. Furthermore, the fractures of the forearm, femoral neck, lumbar spine, heel corresponding with the BMD regions, and femoral neck and lumbar spine BMD in men and women, separately, were added as additional outcomes. Two-sample MR approaches were utilized in this study. Inverse variance weighting (IVW) was adopted as the main analysis. MR-PRESSO, MR-Egger, the weighted median estimate, and multivariable MR were performed as sensitivity methods.

Results

In the main analysis, Hcy concentrations have an inverse association with heel BMD (Beta = 0.046, 95% confidence interval (CI) -0.073 to -0.019, P = 9.59E-04) per SD unit. In addition, for one SD increase of vitamin B12, the total body BMD decreased 0.083 unit (95%CI -0.126 to -0.040, P = 1.65E-04). The trend was more obvious in age over 45 years (Beta = -0.135, 95%CI -0.203–0.067, P = 9.86E-05 for age 45-60; Beta = -0.074, 95%CI -0.141 to -0.007, P = 0.031 for age over 60 years). No association of B vitamins and Hcy levels with the risk of fractures and femoral neck and lumbar spine BMD in men and women was found in this study. Other sensitivity MR methods elucidated consistent results.

Conclusions

Our findings indicated that there exist the inversely causal effects of Hcy and vitamin B12 on BMD in certain body sites and age strata. These give novel clues for intervening bone-related diseases in public health and nutrition.
Combination of transsphenoidal endoscopic surgery and presurgical somatostatin analogs in thyrotropin (TSH)-secreting pituitary adenomas: Treatment outcome and long-term remission at a single pituitary center
Authors: Liu Jie , Yang Yamei , Duan Lian , Chai Xiaofeng , Zhu Huijuan , Deng Kan , Lian Xiaolan , Yao Yong
Published: November 2022

Background

Thyrotropin (TSH)-secreting pituitary adenomas (TSHomas) account for an extremely rare group of pituitary adenomas. Few studies examined the sensitivity and efficacy of presurgical somatostatin analogs (SSAs) and described the long-term remission under such treatment modality. The aim of the present study was to assess the efficacy of presurgical SSA treatment and long-term remission after surgery.

Methods

A retrospective cohort of 65 TSHoma patients who received endoscopic transsphenoidal pituitary surgery between 2011 and 2020 in a single pituitary center in China was established. Data were analyzed for sex differences and different types of SSA and ultimately to explore the hormonal cutoff for remission prediction.

Results

TSHomas had a predominant female preference in this cohort (43 women vs. 22 men). Baseline FT3 was higher in men [7.543 ± 2.407 vs. 5.58 (4.99, 6.58), p = 0.019], which was consistent with its longer diagnosis time and larger tumor volume. The median medication time for hormonal control was 2. 5 days for short-acting SSA and 4. 0 weeks for long-term SSA. Patients with long-acting SSA had a shrinking maximum tumor diameter at a median of 1.0 (−1.6, 4.925) mm. Only 10 patients (15.38%) were not in complete remission among whom 8 patients were not en-bloc resected and 2 patients had tumor recurrence after 81.6 and 10. 7 months of complete removal. Postsurgical thyroid hormones (within 1 week) of TSH <0.094 μIU/ml were identified as the cutoff for remission using the ROC curve.

Conclusions

The combination of endoscopic transsphenoidal surgery and presurgical SSA TSHomas provided a higher long-term remission for TSHomas.
On the altitude profile of lower ionospheric D-region response time delay during solar flares
Authors: Chakraborty Sayak , Paul Rakhi , Basak Tamal
Published: November 2022

The D-region ionosphere is sluggish in nature while responding to the incoming solar radiation. We study the altitude (h) profile of mid-latitude D-region response time delay (Δt) during three chosen solar flares, namely, C5.2, M5.2, and X2.2 classes. We solve “electron continuity equation” numerically at each and every h to obtain Δt-h profile. We investigate the 1) latitudinal variation (over both Northern and Southern hemispheres) and 2) seasonal variation (throughout the year) of Δt-h profiles of each of these solar flares separately. We go over noteworthy variations of Δt-h profile for both 1) and 2) with reasonably different results over different hemispheres. Also, we study some contrasting behaviours of Δt-h profile of X2.2 class for both 1) and 2) in comparison to C5.2 or M5.2 classes. We conclude the outcome with possible explanations.
Status quo and utilization trend of global helium resources
Authors: Cao Qian , Li Ying , Fang Chaohe , Liu Renhe , Xiao Hongping , Wang Shejiao
Published: November 2022

Helium, as an important strategic resource, is widely used in medical and sophisticated high-tech, but its distribution is seriously imbalanced, mainly concentrated in the United States, Qatar, Algeria and Russia. In this paper, based on the systematic study of the global distribution and supply and demand of helium, the current situation and future trend of helium consumption in china were analyzed. In view of the shortage of helium resources in China and the high dependence on foreign countries, suggestions were put forward to strengthen the comprehensive research on domestic helium resources, enhance the investigation and utilization of associated helium resources, deepen the research on helium exploration and development technology, and promote the process of commercial exploration and development.
Simulation of water resources carrying capacity of the Hangbu River Basin based on system dynamics model and TOPSIS method
Authors: Liu Yang , Gao Cheng , Ji Xiaomin , Zhang Zeyi , Zhang Yuquan , Liu Chenwei , Wang Zhenxing
Published: November 2022

Water resources play a key role in development. The research on water resources carrying capacity (WRCC) is helpful to judge the development status and provide suggestions for regional policy-making. In this study, the System Dynamics (SD) model and the Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS) method are combined to calculate the change of WRCC of the Hangbu River basin from 2015 to 2035 under four development scenarios. The results show that WRCC of each scenario decreases first and then increases. The scenario that prioritizes water resources is the best for the Hangbu River Basin, under which the WRCC will increase to 0.631 in 2035, achieving a good level. This is different from other WRCC studies. Through analysis, the research method and the development level and focus of the study area may be the reasons for this difference. In addition, some suggestions were provided such as focusing on improving the efficiency of irrigation water use and looking at the long-term development of the region.
Spatial analysis and risk assessment of heavy metal pollution in rice in Fujian Province, China
Authors: Guo Yourui , Huang Mingtian , You Wu , Cai Luxiang , Hong Yong , Xiao Qingtie , Zheng Xinyu , Lin Ruiyu
Published: November 2022

To comprehensively understand the spatial characteristics and pollution hazards of heavy metals in rice in Fujian Province, the contents of Cd, Hg, As, Pb, Cr, Ni, Cu, and Zn in 260 rice samples were measured based on the field investigation. Their spatial distribution patterns and clustering characteristics were explored by geostatistical methods, and their pollution-health risks were revealed by the pollution index method and exposure evaluation model. The results showed that the average contents of eight heavy metals in rice were below the food safety limits, but the excess rates of As, Cu, and Cd samples reached 33.08%, 22.69%, and 18.85%, respectively. In terms of spatial characteristics, the contents of Cd, Hg, and Ni were higher in the southeastern and northwestern regions, while the contents of As, Cu, and Cr were higher in the southwestern parts of Fujian Province. The hotspots of the corresponding heavy metals were also concentrated in the above-mentioned areas, showing an obvious spatial clustering effect. The pollution risk assessment showed that the single factor pollution index of heavy metals in rice decreased in the order of As (0.536) > Hg (0.505) > Cu (0.421) > Cd (0.378) > Cr (0.318) > Zn (0.304) > Pb (0.286) > Ni (0.116), and the Nemerow comprehensive pollution index was 0.623, which was generally at a safe level with low pollution risk. Furthermore, the non-carcinogenic risk index of heavy metals for adults and children were 3.558 and 6.014, and the carcinogenic risk index were 0.0050 and 0.0084, respectively, with extremely serious health hazards. In contrast, the health damage of heavy metals was higher in children than that in adults, with As and Cd in rice as the pivotal elements. Therefore, attention should be paid to the future monitoring and agricultural management of heavy metal pollution in rice in the region.
Biomass burning-agriculture coupling in the Orinoco savannas—Particulate matter emission scenarios
Authors: Jimenez Rodrigo , Ardila Andres V. , Vargas-Burbano Angela C. , Hernandez Andrea J. , Leon-Velasquez Elizabeth
Published: November 2022

The Colombian Orinoco savannas (254 thousand km2), also known as Orinoquia or Llanos, have been steadily transformed into pastures for more than a century, and since the 1990s, into commodity crop intensified production. The cropland area expanded at 12% yr−1 during the 2007–2018 period (65% larger than in 1996–2007). Yet, we estimate that cattle ranching occupied ten times more area (34%) than cropland (3.2%) in 2018. The rest of Orinoquia, including indigenous reservations and protected areas, was in a semi-natural state, although also exposed to seasonal fire. The three main crops, oil palm, corn, and rice (72% of the sown area in 2017), accounted for 68% of the expansion, with permanent crops expanding two times faster (18% yr−1) than short-cycle crops. An extrapolation of trends indicates that the cultivated area will double by 2040 (reaching 20 thousand km2), with oil palm as the dominant crop. Satellite measurements show that 7% of Orinoquia burned every year during the 1997–2016 period, yet with large spatial and interannual variations (±26%), and significant decrease trends (up to −4% yr−1). Up to 40% of the burned area (BA) interannual variability was linked to irregular rainfall and drought. The areas with the larger fractional BA were also those with the least fractional cropland cover. A model developed to describe this coupling, along with rainfall and other effects, successfully explained most of Orinoquia’s BA variability (r2 = 0.93). The fitted model indicates that each sown hectare reduced the BA by 0.17 ha. This model predicts that the combination of cropland expansion and independent BA decline will lead to a fourfold reduction of Orinoquia’s BA by 2040 referred to 1997. Orinoquia’s crop production generated 3 Gg of PM10 (particulate matter <10 µm) in 2016, mostly from short-cycle crops, while biomass burning generated 57 Gg, i.e., 95% of the combined emissions. These are expected to halve during the 2017–2040 period, despite an 83% increase in crop production emissions, as total and seasonal emissions will remain controlled by biomass burning. Such a large pollution burden reduction should have tremendous positive impacts on public health in Orinoquia and the Andes.
Duration of the susceptibility of pruning wounds of different ages to infections by Phaeomoniella chlamydospora on grapevine cv. Cabernet Sauvignon in Central Chile
Authors: Díaz Gonzalo A. , Latorre Bernardo A.
Published: November 2022

Grapevine trunk diseases (GTDs) are one of the most important phytosanitary problems that affect grapevines (Vitis vinifera) worldwide. In Chile, Phaeomoniella chlamydospora is the major fungal trunk pathogen associated with GTDs. In the vineyards, the natural infections by P. chlamydospora are associated with air-borne conidia dispersed onto fresh pruning wounds from pycnidia. These pruning wounds are considered an important entrance for fungal trunk pathogens such as P. chlamydospora in the host in the field. However, the duration of the susceptibility of grapevine annual pruning wounds to P. chlamydospora is still unknown in Chile. Therefore, this study aimed to evaluate the period of susceptibility of pruning wounds of different ages to artificial infection of P. chlamydospora on grapevine cv. Cabernet Sauvignon, Central Chile. Artificial inoculations of a conidial suspension (105 conidia/mL) of P. chlamydospora were used to determine the susceptibility of pruning wounds of different ages, from 1, 15, 30, and 45 days after pruning. The experiments were conducted on lignified cuttings in a greenhouse, and on vine spurs in two vineyards (Buin and Nancagua, Central Chile) during two consecutive seasons. The results indicated that the pruning wounds of grapevine cv. Cabernet Sauvignon were very susceptible to infections by P. chlamydospora, with a percentage of pruning wounds infected from 97 to 71% for cuttings, and 96% to 60% for spurs, during the first 15 days after pruning. However, the susceptibility of pruning wounds of different ages in cuttings and spurs of grapevine, generally decreased as the time from pruning to inoculation increased. Moreover, the pruning wounds the pruning wounds remained susceptible to artificial inoculation by P. chlamydospora for up 45 days after pruning with percent of wounds infected from 8.0 to 12.2, and 8.3 to 18.8% on cuttings and spurs of grapevine, respectively. Finally, this study constitutes study constitutes the first research focalized on the susceptibility of pruning wounds of various ages of grapevine cv. Cabernet Sauvignon to artificial inoculations by P. chlamydospora in Central Chile.
Baseline immune signature score of Tregs × HLA-DR+CD4+ T cells × PD1+CD8+ T cells predicts outcome to immunotherapy in cancer patients
Authors: Mispelbaum Rebekka , Hattenhauer Sandra Tessa , Held Stefanie Andrea Erika , Brossart Peter , Heine Annkristin
Published: November 2022

Background

The use of immunotherapy (IT) is rapidly increasing across different tumor entities. PD-L1 expression is primarily used for therapy evaluation. The disadvantages of PD-L1 status are spatial and temporal heterogeneity as well as tumor type-dependent variation of predictive value. To optimize patient selection for IT, new prediction markers for therapy success are needed. Based on the systemic efficacy of IT, we dissected the immune signature of peripheral blood as an easily accessible predictive biomarker for therapeutic success.

Methods

We conducted a retrospective clinical study of 62 cancer patients treated with IT. We assessed peripheral immune cell counts before the start of IT via flow cytometry. The predictive value for therapy response of developed immune signature scores was tested by ROC curve analyses and scores were correlated with time to progression (TTP).

Results

High score values of “Tregs ÷ (CD4+/CD8+ ratio)” (Score A) and high score values of “Tregs × HLA-DR+CD4+ T cells × PD1+CD8+ T cells” (Score B) significantly correlated with response at first staging (p = 0.001; p < 0.001). At the optimal cutoff point, Score A correctly predicted 79.1% and Score B correctly predicted 89.3% of the staging results (sensitivity: 86.2%, 90.0%; specificity: 64.3%, 87.5%). A high Score A and Score B statistically correlated with prolonged median TTP (6.13 vs. 2.17 months, p = 0.025; 6.43 vs. 1.83 months, p = 0.016). Cox regression analyses for TTP showed a risk reduction of 55.7% (HR = 0.44, p = 0.029) for Score A and an adjusted risk reduction of 73.2% (HR = 0.27, p = 0.016) for Score B.

Conclusion

The two identified immune signature scores showed high predictive value for therapy response as well as for prolonged TTP in a pan-cancer patient population. Our scores are easy to determine by using peripheral blood and flow cytometry, apply to different cancer entities, and allow an outcome prediction before the start of IT.
Identification of molecular subtypes based on liquid–liquid phase separation and cross-talk with immunological phenotype in bladder cancer
Authors: Sun Le , Liu Xiao-Ping , Yan Xin , Wu Shaojie , Tang Xiaoyu , Chen Chen , Li Gang , Hu Hankun , Wang Du , Li Sheng
Published: November 2022

Background

Mounting evidence has demonstrated that an imbalance in liquid–liquid phase separation (LLPS) can induce alteration in the spatiotemporal coordination of biomolecular condensates, which plays a role in carcinogenesis and cachexia. However, the role of LLPS in the occurrence and progression of bladder cancer (BLCA) remains to be elucidated. Identifying the role of LLPS in carcinogenesis may aid in cancer therapeutics.

Methods

A total of 1,351 BLCA samples from six cohorts were retrieved from publicly available databases like The Cancer Genome Atlas, Gene Expression Omnibus, and ArrayExpress. The samples were divided into three distinct clusters, and their multi-dimensional heterogeneities were explored. The LLPS patterns of all patients were determined based on the LLPS-related risk score (LLPSRS), and its multifaceted landscape was depicted and experimentally validated at the multi-omics level. Finally, a cytotoxicity-related and LLPSRS-based classifier was established to predict the patient’s response to immune checkpoint blockade (ICB) treatment.

Results

Three LLPS-related subtypes were identified and validated. The differences in prognosis, tumor microenvironment (TME) features, cancer hallmarks, and certain signatures of the three LLPS-related subtypes were validated. LLPSRS was calculated, which could be used as a prognostic biomarker. A close correlation was observed between clinicopathological features, genomic variations, biological mechanisms, immune infiltration in TME, chemosensitivity, and LLPSRS. Furthermore, our classifier could effectively predict immunotherapy response in patients with BLCA.

Conclusions

Our study identified a novel categorization of BLCA patients based on LLPS. The LLPSRS could predict the prognosis of patients and aid in designing personalized medicine. Further, our binary classifier could effectively predict patients’ sensitivity to immunotherapy.
Application of light-emitting diodes (LEDs) fishing lights to improve catch rates of small-scale trammel net fishery in the Yellow Sea, China
Authors: Yu Mengjie , Liu Changdong , Zhang Liyou , Tang Yanli
Published: November 2022

The small-scale trammel net fisheries are essential to the economy and well-being of coastal communities in the Yellow Sea, China. However, these fisheries are low efficient and urgently demand technical innovations to improve catch rates. In this study, we conducted field experiments in three traditional fishing grounds (Rizhao, Yantai, and Weihai) to test whether the addition of different colored light-emitting diodes (LEDs) fishing lights in the trammel nets could improve the catch rates of seven commercial species. Results showed that the effects of LEDs on catch rates were species-specific. Green LEDs could significantly increase the catch per unit effort (CPUE) of black rockfish (Sebastes schlegelii), fat greenling (Hexagrammos otakii), marbled flounder (Pseudopleuronectes yokohamae), stone flounder (Platichthys bicoloratus), Asian paddle crab (Charybdis japonica), and mantis shrimp (Oratosquilla oratoria) by 40%-72% compared with unilluminated nets, while had no significant effect on the catch rates of swimming crab (Portunus trituberculatus). Red LEDs harvested 58%-86% higher CPUE of fat greenling, marbled flounder, stone flounder, mantis shrimp, and swimming crab while catching fewer black rockfish and Asian paddle crab. Blue and white LEDs exhibited similar performance, which could significantly capture more black rockfish, fat greenling, and mantis shrimp and did not affect the catch rates of other species. The effects of LEDs were also size-dependent for certain species, and the catch rates generally increased with the length classes. Taking economic benefits into consideration, the green and red LEDs provided the highest profitability for Rizhao, while green LEDs performed best in Weihai and Yantai. This study is the first investigation on how artificial lights affected the catch rates of trammel nets. The findings of our study illustrated that trammel nets equipped with LEDs could serve as a viable fishing strategy in small-scale trammel net fisheries.
Where and how - new insight for brown algal forest restoration in the Adriatic
Authors: Orlando-Bonaca Martina , Savonitto Gilda , Asnaghi Valentina , Trkov Domen , Pitacco Valentina , Šiško Milijan , Makovec Tihomir , Slavinec Petra , Lokovšek Ana , Ciriaco Saul , Chiantore Mariachiara , Kaleb Sara , Descourvières Emmanuelle Patricia , Srijemsi Marina , Falace Annalisa
Published: November 2022

Several anthropogenic factors are responsible for the decline of Cystoseira sensu lato (hereafter Cystoseira) forests along Mediterranean coasts. Some Cystoseira species are already regionally extinct, and their decline has been widely recorded. Sustainable and efficient techniques for the restoration of Cystoseira are needed. In this context, the objectives of this study were i) to analyse the reproductive traits of three populations of Gongolaria barbata from three nearby donor sites in the northern Adriatic Sea, assessing the differences in their reproductive potential and reproductive success; and ii) to evaluate the outplanting success in terms of the effectiveness of G. barbata restoration, in relation to the different donor and receiving sites (Miramare MPA and in front of the Marine Biology Station Piran - MBSP) and implemented methods (ex situ and hybrid method combining a mesocosm cultivation and a suspended culture in the field). After 2 weeks of cultivation in mesocosms, half of the tiles with germlings were transported to the receiving sites and placed on suspended lantern nets (hybrid method), which were later (after 3 months) transferred to the seabed on concrete plates with protective cages. The remaining tiles were placed on the seabed on concrete plates with protective cages after a 4-week culture (ex situ method). At both sites, lantern nets and plates were randomly placed at 3 m depth. Thallus length was measured monthly in each treatment. Seedlings in suspended culture showed lower performance at the Miramare MPA, most likely due to the unfavourable environmental conditions. The satisfactory results obtained at MBSP demonstrate the efficiency of the hybrid method and confirm its potential to reduce the cost and time required for cultivation. Since unpredictable climatic events pose the greatest threat to restoration performance, these challenges must be considered when establishing new restoration practices. Moreover, herbivore regulation is extremely urgent and should be planned and implemented on a larger regional scale.
Comparative structural analysis on the mitochondrial DNAs from various strains of Lentinula edodes
Authors: Kim Sinil , Eom Hyerang , Nandre Rutuja , Choi Yeon Jae , Lee Hwayong , Ryu Hojin , Ro Hyeon-Su
Published: November 2022

The evolution of mitochondria through variations in mitochondrial DNA (mtDNA) is one of the intriguing questions in eukaryotic cells. In order to assess the causes of the variations in mitochondria, the mtDNAs of the 21 strains of Lentinula edodes were assembled for this study, and analyzed together with four published mtDNA sequences. The mtDNAs were within the sizes of 117 kb ~ 122 kb. The gene number was observed consistent except for two mtDNAs, which carry a duplicated trnG1-trnG2 unit or a putative gene deletion. The size variation was largely attributed to the number of introns, repeated sequences, transposable elements (TEs), and plasmid-related sequences. Intron loss and gain were found from cox1, rnl, and rns of three mtDNAs. Loss of two introns in cox1 of KY217797.1 reduced its size by 2.7 kb, making it the smallest cox1 gene (8.4 kb) among the cox1s of the 25 mtDNAs, whereas gain of a Group II intron (2.65 kb) and loss of a Group I intron (1.7 kb) in cox1 of MF774813.1 resulted in the longest cox1 (12 kb). In rnl of L. edodes, we discovered four intron insertion consensus sequences which were unique to basidiomycetes but not ascomycetes. Differential incorporation of introns was the primary cause of the rnl size polymorphism. Homing endonucleases (HEGs) were suggestively involved in the mobilization of the introns because all of the introns have HEG genes of the LAGRIDADG or GIY-YIG families with the conserved HEG cleavage sites. TEs contributed to 11.04% of the mtDNA size in average, of which 7.08% was LTR-retrotransposon and 3.96% was DNA transposon, whereas the repeated sequences covered 4.6% of the mtDNA. The repeat numbers were variable in a strain-dependent manner. Both the TEs and repeated sequences were mostly found in the intronic and intergenic regions. Lastly, two major deletions were found in the plasmid-related sequence regions (pol2-pol3 and pol1-atp8) in the five mtDNAs. Particularly, the 6.8 kb-long deletion at pol2-pol3 region made MF774813.1 the shortest mtDNA of all. Our results demonstrate that mtDNA is a dynamic molecule that persistently evolves over a short period of time by insertion/deletion and repetition of DNA segments at the strain level.
Contribution of gut microbiomes and their metabolomes to the performance of Dorper and Tan sheep
Authors: Ma Yuhao , Yang Xue , Hua Guoying , Deng Xiaotian , Xia Tianlan , Li Xinhai , Feng Dengzhen , Deng Xuemei
Published: November 2022

Background

Livestock is an excellent source of high nutritional value protein for humans; breeding livestock is focused on improving meat productivity and quality. Dorper sheep is a distinguished breed with an excellent growth performance, while Tan sheep is a Chinese local breed famous for its delicious meat. Several studies have demonstrated that the composition of gut microbiome and metabolome modulate host phenotype.

Methods

In the present study, we performed 16S amplicon sequencing and metabolomic analyses of the rumen and hindgut microbiome of 8-month-old Dorper and Tan sheep, raised under identical feeding and management conditions, to explore the potential effects of gut microbiome and its metabolites on growth performance and meat quality.

Results

Our study identified Lactobacillus, a marker genus in the rumen, to be significantly associated with the levels of fumaric acid, nicotinic acid, and 2-deoxyadenosine (P-value < 0.05). Statistical analysis showed that nicotinic acid was significantly negatively correlated with body weight (P-value < 0.01), while 2-deoxyadenosine was significantly positively correlated with fatty acids content (P-value < 0.05). There was a biologically significant negative correlation between Phascolarctobacterium and deoxycytidine levels in the hindgut. Deoxycytidine was significantly positively correlated with body weight, protein, and amino acid content. Differences in rumen fermentation patterns that are distinctive among breeds were identified. Tan sheep mainly used Lactobacillus and fumaric acid-mediated pyruvic acid for energy supply, while Dorper sheep utilize glycogenic amino acids. The difference of iron metabolism in the hindgut of Dorper sheep affects lipid production, while Phascolarctobacterium in Tan sheep is related to roughage tolerance. The accumulation of nucleosides promotes the growth performance of Dorper sheep.

Conclusion

These findings provide insights into how the microbiome-metabolome-dependent mechanisms contribute to growth rate and fat contents in different breeds. This fundamental research is vital to identifying the dominant traits of breeds, improving growth rate and meat quality, and establishing principles for precision feeding.
Analysis of subgingival micro-organisms based on multi-omics and Treg/Th17 balance in type 2 diabetes with/without periodontitis
Authors: Jiang Lanlan , Zhang Jiaming , Fang Meifei , Qin Yingfen , Huang Yuxiao , Tao Renchuan
Published: November 2022

Type 2 diabetes mellitus (T2DM) and periodontitis are common and interrelated diseases, resulting in altered host response microbiota. The subgingival micro-organisms play a key role in periodontitis pathogenesis. To assess the shift of subgingival microbiome and metabolome in T2DM, we performed an analysis of the subgingival microbiome in patients with T2DM (n = 20) compared with non-diabetes (ND) subjects (n = 21). Furthermore, patients were subdivided into 10 T2DM with periodontitis (DP), 10 T2DM without periodontitis (DNP), 10 periodontitis (P), and 11 healthy control (H) groups. 16SrRNA gene sequencing combined with ultra high-performance liquid chromatography-mass spectrometry (UHPLC–MS) based metabolomics was performed in all participants. T lymphocyte immunity was analyzed by flow cytometry. Furthermore, the network relationship among subgingival micro-organisms, metabolites, blood glucose level, and T lymphocyte immunity were analyzed. The results showed that the difference of the subgingival microbiome from healthy to periodontitis status was less prominent in T2DM compared with ND, though the clinical signs of disease were similar. The bacteria Eubacterium nodatum group, Filifactor, Fretibacterium, Peptostreptococcus, and Desulfovibrio, amongst others, may be important in the pathopoiesia of periodontitis in the T2DM state. In addition, some dominant bacteria showed network relationships. The Treg/Th17 ratio was lower in the DP and DNP groups than in the P and H groups—though that of P was lower than for H. The percentage of CD4+/CD8+ PD1 and CD8+ PDL1 was higher in the DP and DNP groups than in the H group; the percentage of CD8+ PDL1 was higher in the DP than P groups. Subgingival micro-organisms in periodontitis had a significant metabolic shift in terms of their signature metabolites. Butyrate metabolism and phenylalanine metabolism may play a role in the pathogenesis of periodontitis with/without T2DM. Specifically, biphenyl degradation, tryptophan metabolism, and the two-component system may play important roles in periodontitis with T2DM. Lastly, the network relationship among subgingival micro-organisms, metabolites, blood glucose level, and T lymphocyte immunity were unbalanced. This study identified the changes in the subgingival microbiome associated with periodontitis in T2DM, as well as the associated network between bacterial flora, metabolism dysbiosis, and immune regulation.
Gel polymer electrolyte for reversible metal electrodeposition dynamic windows enables dual-working electrodes for faster switching and reflectivity control
Authors: Cai Yuchun , Hernandez Tyler S. , Yeang Andrew L. , Strand Michael T. , Yavitt F. Max , Abraham Eldho , McGehee Michael D.
Published: November 2022

Dynamic windows based on reversible metal electrodeposition are an attractive way to enhance the energy efficiency of buildings and show great commercial potential. Dynamic windows that rely on liquid electrolytes are at risk of short circuiting when two electrodes contact, especially at larger-scale. Here we developed a poly (vinyl alcohol) (PVA) gel polymer electrolyte (GPE) with 85% transmittance, that is, sufficiently stiff to act as a separator. The GPE is implemented into windows that exhibit comparable electrochemical and optical properties to windows using a liquid electrolyte. Furthermore, the GPE enables the fabrication of windows with dual-working electrodes (WE) and a metal mesh counter electrode in the center without short-circuiting. Our dual-WE PVA GPE window reaches the 0.1% transmittance state in 101 s, more than twice the speed of liquid windows with one working electrode (207 s). Additionally, each side of the dual-WE GPE window can be tinted individually to demonstrate varied optical effects (i.e., more reflective, or more absorptive), providing users and intelligent building systems with greater control over the appearance and performance of the windows in a single device architecture.
Different assessment tools to detect sarcopenia in patients with Parkinson's disease
Authors: Valent Dora , Peball Marina , Krismer Florian , Lanbach Anna , Zemann Sophie , Horlings Corinne , Poewe Werner , Seppi Klaus
Published: November 2022

Introduction

Sarcopenia and Parkinson's disease are closely related diseases of the elderly population leading to progressive disability and nursing-dependent care.

Objective

The aim of this study was to estimate the prevalence of sarcopenia in PD patients with three different approaches: (1) the screening tool SARC-F, (2) EWGSOP-1 criteria, and (3) EWGSOP-2 criteria. Moreover, we aimed to evaluate the diagnostic accuracy of the screening tool SARC-F to detect sarcopenia according to the updated EWGSOP-2 criteria.

Methods

Eighty-one patients with Parkinson's disease aged 65 years and above were interviewed in a cross-sectional study at a tertiary referral center. All patients were screened with the SARC-F questionnaire and were evaluated for motor and non-motor symptoms, exercise, quality of life, and frailty. Muscle mass was assessed with bioelectrical impedance analysis, handgrip strength with a dynamometer, and gait speed was assessed with the 8-m walk test. EWGSOP-2 criteria were considered the gold standard to diagnose sarcopenia in our study.

Results

Eighty-one patients were evaluated (mean age: 73.82; SD 5.30). The prevalence of sarcopenia was 28.4% according to the EWGSOP-2 criteria. The concordance between EWGSOP-2 and EWGSOP-1 was poor (weighted kappa of 0.361[95% 0.164–0.557]). The sensitivity of the SARC-F screening test for detecting sarcopenia was 60.9%. The corresponding AUC in the ROC curve analysis showed 0.598 (0.462, 0.734 CI). The item assessing strength was found to have the highest sensitivity (69.6%).

Conclusion

Sarcopenia prevalence in patients with PD in Tirol, Austria is higher with EWGSOP-1 criteria compared to EWGSOP-2 criteria. The sensitivity and specificity of the SARC-F scale to detect sarcopenia in this population are poor.
HCC biomarkers – state of the old and outlook to future promising biomarkers and their potential in everyday clinical practice
Authors: Schlosser Sophie , Tümen Deniz , Volz Barbara , Neumeyer Katja , Egler Niklas , Kunst Claudia , Tews Hauke Christian , Schmid Stephan , Kandulski Arne , Müller Martina , Gülow Karsten
Published: November 2022

Hepatocellular carcinoma (HCC) is one of the most common and deadly tumors worldwide. Management of HCC depends on reliable biomarkers for screening, diagnosis, and monitoring of the disease, as well as predicting response towards therapy and safety. To date, imaging has been the established standard technique in the diagnosis and follow-up of HCC. However, imaging techniques have their limitations, especially in the early detection of HCC. Therefore, there is an urgent need for reliable, non/minimal invasive biomarkers. To date, alpha-fetoprotein (AFP) is the only serum biomarker used in clinical practice for the management of HCC. However, AFP is of relatively rather low quality in terms of specificity and sensitivity. Liquid biopsies as a source for biomarkers have become the focus of clinical research. Our review highlights alternative biomarkers derived from liquid biopsies, including circulating tumor cells, proteins, circulating nucleic acids, and exosomes, and their potential for clinical application. Using defined combinations of different biomarkers will open new perspectives for diagnosing, treating, and monitoring HCC.
LDHA: The Obstacle to T cell responses against tumor
Authors: Tang Yu , Gu Shuangshuang , Zhu Liqun , Wu Yujiao , Zhang Wei , Zhao Chuanxiang
Published: November 2022

Immunotherapy has become a successful therapeutic strategy in certain solid tumors and hematological malignancies. However, this efficacy of immunotherapy is impeded by limited success rates. Cellular metabolic reprogramming determines the functionality and viability in both cancer cells and immune cells. Extensive research has unraveled that the limited success of immunotherapy is related to immune evasive metabolic reprogramming in tumor cells and immune cells. As an enzyme that catalyzes the final step of glycolysis, lactate dehydrogenase A (LDHA) has become a major focus of research. Here, we have addressed the structure, localization, and biological features of LDHA. Furthermore, we have discussed the various aspects of epigenetic regulation of LDHA expression, such as histone modification, DNA methylation, N6-methyladenosine (m6A) RNA methylation, and transcriptional control by noncoding RNA. With a focus on the extrinsic (tumor cells) and intrinsic (T cells) functions of LDHA in T-cell responses against tumors, in this article, we have reviewed the current status of LDHA inhibitors and their combination with T cell-mediated immunotherapies and postulated different strategies for future therapeutic regimens.