bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of variability in reproductive traits and breeding efficiency in rabbits (Oryctolagus cuniculus L. 1758) of the Popielno White and Termond White breeds
Authors:   Institute of Zootechnics and Fisheries, Faculty of Agrobioengineering and Animal Husbandry, University in Siedlce, B. Prusa 14, 80-110 Siedlce, Poland, Socha Stanisław , Weremczuk Dorota Elżbieta ,   Institute of Zootechnics and Fisheries, Faculty of Agrobioengineering and Animal Husbandry, University in Siedlce, B. Prusa 14, 80-110 Siedlce, Poland, Kołodziejczyk Dorota ,   Institute of Zootechnics and Fisheries, Faculty of Agrobioengineering and Animal Husbandry, University in Siedlce, B. Prusa 14, 80-110 Siedlce, Poland
Published: June 2024

In the study, the variability of reproductive traits (the number of born and weaned young) and the efficiency of breeding work were assessed and estimated in rabbits (Oryctolagus cuniculus L. 1758) of the Popielno White and Termond White breeds. Phenotypic trends of reproductive traits were estimated over a period of ten years in farms included in the analysis. The research material included data on the performance of 1836 female rabbits of the Termond White breed and 1237 female rabbits of the Popielno White breed, maintained on reproductive farms over a period of ten years. Analysis of variance for reproductive traits showed that sources of variability (farm, calendar year, and breed) had a statistically significant impact on the number of born and weaned rabbits. The average litter size obtained from female Termond White rabbits ranged from 2.89 to 3.54 within one year. For female Popielno White rabbits, this average was 3.44. The average number of born and weaned kits per litter during the analyzed period was 8.99 and 8.29, respectively. The reproductive results obtained regarding the number of born and weaned rabbits can be considered very good. The phenotypic trend line for the number of born and raised rabbits of the white Popielno breed showed an increasing tendency, while in the case of rabbits of the white Termond breed, it exhibited a decreasing trend. Clear fluctuations in the number of born and raised rabbit kits in the Termond white breed throughout the research period indicate the need to improve the reproductive traits of this breed through intensified selection. In the light of our own research, it can be stated that rabbit breeding in both farms is conducted properly, and the obtained results are satisfactory.
Educational Tool for Determining Viscosity Coefficient of Cooking Oil Using Arduino UNO
Authors: Hilyana F. Shoufika , Marwoto Putut , Nugroho Sunyoto Eko
Published: June 2024

The research aims to determine the quality of cooking oil based on the viscosity coefficient using an Arduino UNO-based viscosity coefficient determining tool. By integrating the Arduino UNO into the viscosity measurement process, this research shows innovation in adapting widely available technology for scientific purposes. The series of tools for determining the value of the viscosity coefficient work if an iron ball enters the fluid and will be detected by magnetic sensors 1 and 2 if it passes through the sensor, which is placed at a predetermined distance. Research was conducted to test the quality of cooking oil by determining the viscosity coefficient. The method research is analyzing recorded videos of the motion of objects falling in oil with Tracker Software. By tracking the video recording of the motion of an object falling in a liquid, data, and graphs of the speed of the falling motion can be obtained. Then from the data obtained, the viscosity value is obtained using the equation. The results of the 5 cooking oils, using the tool, were that oil 1 had a viscosity coefficient of 0.2732 Pa.s; oil 2 with 0.3077 Pa.s; oil 3 with 0.3127 Pa.s; oil 4 with 0.3186; and oil 5 with 0.3218 Pa.s. Meanwhile, the results of the oil viscosity coefficient using manual calculations showed that oil 1 had 0.2733 Pa.s; oil 2 with 0.3089 Pa.s; oil 3 with 0.3136 Pa.s; oil 4 with 0.3188; and oil 5 with 0.3219 Pa.s. The percentage of error that occurs from the tool is 0.17%. The oil with a smaller viscosity coefficient is coconut oil (better quality than palm oil), while the quality of bulk oil is indicated by a larger viscosity coefficient. It could be asserted that the oil's quality improves as the viscosity coefficient decreases.
Kudret Altun, [Osmanlı’da Sosyal Sınıf ve Kimlikler], Bilge Kültür Sanat Yayınevi, İstanbul, 2019.
Authors: Karaoğulları Nurullah
Published: June 2024

Osmanlı ahalisinde her ferdin kendini ifade etme, kendi değerlerini yaşama ve kültürel özelliklerini sergileyebilme imkânına sahip olduğu bir atmosfer hâkimdi. Bu durumu farklı ritüellerin yapılmasında, birbirinden farklı kıyafetlerin giyilmesinde, alışkanlıkların çeşitliliğinde görebilmek mümkündür. Osmanlı’da farklı sosyal tabakalara mensup fertlerin aidiyetleri çerçevesinde kullandıkları başlıklar da kültürel genişliğin bir göstergesi olarak tebarüz etmektedir. Bu çalışmada Prof. Dr. Kudret Altun tarafından yazılan “Osmanlı’da Sosyal Sınıf ve Kimlikler” adlı eser tanıtılmaktadır. Eserde sözü edilen başlıklara yönelik İtalyan yazar ve devlet adamı Luigi Ferdinand Marsigli’nin çabaları sonucu vücuda gelen “Marsigli Koleksiyonu”ndan seçkin bir albüm ele alınmaktadır. Osmanlı toplumunda sosyal sınıfların ve tabakaların işlevselliği ile ilgili önemli bilgiler veren eser, meslek grupları ile ilgili de verileri içermektedir. Görsellerin ve kısa açıklayıcı yazıların canlılıkla sunulduğu eserde günümüz Türkçesiyle de açıklamalar bulunmaktadır. “Osmanlı’da Sosyal Sınıf ve Kimlikler” tarih bağlamında önemli bir kaynak olmasının yanında eğitim-öğretimin değişik kademelerinde faydalanılabilecek bir eser olarak düşünülmektedir. Bu bakımdan tarih ve kültürümüze merak duyan herkesin ve eğitim camiasının istifadesine sunulmuştur.
Another Look to Exercise Prescription: Exercise Timing and the Circadian Clock in 2 Individuals with Type 2 Diabetes and those at risk
Authors: Magalhães João , Oliveira Estela C. , Hetherington-Rauth Megan , Jesus Filipe , Faria Maria Clarissa , Raposo João F. , Ribeiro Rogério T. , Caetano Cristina , Sardinha Luís B.
Published: June 2024

Background: Exercise is known to provide multiple metabolic benefits such as improved insulin sensitivity and glucose control in individuals with type 2 diabetes mellitus (T2DM) and those at risk. Beyond the traditional exercise dose, exercise timing is perceived as a contemporary hot-topic, especially in the field of T2DM, however, the number of intervention studies assessing exercise timing and glucose metabolism is scarce. Our aim is to test the effect of exercise timing (i.e., morning, afternoon, or evening) on the inter-individual response variability in glycemic control and related metabolic health parameters in individuals with T2DM and those at risk during a 12-week intervention.
Methods: A randomized cross-over exercise intervention will be conducted involving 2 groups: Group 1, individuals with T2DM; Group 2, age-matched older adults with overweight/obesity. The intervention will consist of 3, 2-week blocks of supervised post-prandial exercise using high-intensity interval training (HIIT). Between each training block, a 2-week washout period, where participants avoid structured exercise, will take place. Assessments will be conducted in both groups before and after each exercise block. The primary outcomes include the 24-hour area under the curve continuous glucose monitoring based glucose. The secondary outcomes include body composition, resting energy expenditure, insulin response to a meal tolerance test, maximal aerobic capacity, peak power output, physical activity, sleep quality, and insulin and glucose levels. All primary and secondary outcomes will be measured at each assessment point.
Discussion: Outcomes from this trial will provide us additional insight into the role of exercise timing on the inter-individual response variability in glycemic control and other related metabolic parameters in two distinct populations, thus contributing to the development of more effective exercise prescription guidelines for individuals with T2DM and those at risk.
Trial registration: This study was retrospectively registered, and the details are at: https://clinicaltrials.gov/study/NCT06136013; Registered 18 November 2023.
Coordinated Computations for Naturalistic Memory Encoding and Retrieval in Hippocampal Neural Subspaces
Authors: Shim Won Mok , Kwon Dasom , Kim Jungwoo , Yoo Seng Bum
Published: June 2024

Our naturalistic experiences are organized into memories through multiple processes, including novelty encoding, memory formation, and retrieval. However, the neural mechanisms coordinating these processes remain elusive. Using fMRI data acquired during movie viewing and subsequent narrative recall, we examined hippocampal neural subspaces associated with distinct memory processes and characterized their relationships. We quantified novelty in character co-occurrences and the valence of relationships and estimated event memorability. Within the hippocampus, the novelty subspaces encoding each type exhibited partial overlap, and these overlapping novelty subspaces aligned with the subspace involved in memorability. Notably, following event boundaries, hippocampal states within these subspaces aligned inversely along a shared coding axis, predicting subsequent recall performance. This novelty-memorability alignment was selectively observed during encoding but not during retrieval. Our findings offer novel insights into how the hippocampus dynamically coordinates computations underlying memory encoding and retrieval at the population level to transform ongoing experiences into enduring memories.
Periodic Relations between Vegetation Cover and Climatic Factors in East Khasi Hills, Meghalaya, India
Authors: Gautam Nimish Narayan , Lhouvum Thangjalal , Warbah Niewkor
Published: June 2024

This study conducts a comprehensive investigation into the vegetation cover dynamics and climatic factors influencing the East Khasi Hill district. Employing a combination of quantitative and qualitative methodologies, the research spans seven years, from 1993 to 2022, integrating primary data from satellite images and secondary climatologically data. The methodology involves a comparison approach utilizing the Normalized Difference Vegetation Index (NDVI) and rule-based classification for vegetation cover analysis at five-year intervals. Optical satellite images from Landsat, along with Terra MODIS LST data, provide critical insights into the land surface conditions. Additionally, ASTER data is employed for automatic stream network extraction and analysis. Elevation and slope classifications offer a spatial understanding of the geographical distribution across different elevation ranges and slope categories. The results indicate a declining trend in vegetation cover, with elevation-wise variations and a mix of gentle to moderately steep slopes in the region. Furthermore, the extraction of Land Surface Temperature (LST) from MODIS, Landsat-5, and Landsat-8 data reveals temperature variations that influence vegetation growth patterns. Monthly and annual climatological data from the India Meteorological Department contribute to the verification of temporal climatic factors, providing valuable context to the vegetation dynamics. The study's findings underscore the dynamic interplay between vegetation cover, topography, and climatic conditions in the East Khasi Hill district. These insights are crucial for informed decision-making in land management, conservation efforts, and understanding the region's environmental changes. The presented methodological flowchart visually summarizes the research approach, providing a roadmap for future studies in similar contexts.
NOD2 reduces the chemoresistance of melanoma by
inhibiting the TYMS/PLK1 signaling axis
Authors: Zhu chun , Yun Fang , Wu Na , Yi Xiao , Zhang Xue , Feng Yu , Ni Qin , Gai Yan , Li En , Yang Zhe , Zhang Qiao , Sai Buqing , Kuang Ying
Published: June 2024

Nucleotide-binding oligomerization domain 2 (NOD2) is an immune sensor crucial for eliciting the innate immune responses. Nevertheless, discrepancies exist regarding the effect of NOD2 on different types of cancer. The aim of this study was to investigate the function of NOD2 in melanoma and its underlying mechanisms. We have validated the tumor suppressor effect of NOD2 in melanoma. NOD2 inhibited the proliferation of melanoma cells, hindering their migration and invasion while promoting the onset of apoptosis. Our study showed that NOD2 expression is closely related to folate metabolism, and its mechanism of action is to inhibit TYMS expression by promoting ubiquitination modification of thymidylate synthase (TYMS), thereby decreasing the resistance of melanoma cells to 5-fluorouracil (5-FU) and capecitabine (CAP). TYMS was identified to form a complex with Polo-like Kinase 1 (PLK1) and activate the PLK1 signaling pathway. Furthermore, we revealed that the combination of the PLK1 inhibitor volasertib (BI6727) with 5-FU or CAP had a synergistic effect repressing the proliferation and migration of melanoma cells. Overall, our research highlights the protective role of NOD2 in melanoma and suggests that targeting NOD2 and the TYMS/PLK1 signaling axis is a high-profile therapy that could be a prospect for melanoma treatment.
Identification of cis-Acting Elements Recognized by Transcription Factor LlWOX11 in Lilium lancifolium
Authors: Bai Jingyi , Yang Panpan , Bi Mengmeng , Xu Leifeng , Ming Jun
Published: June 2024

WOX transcription factors play important roles in plant developmental processes and mainly bind to the WOX-binding element to regulate gene expression. Previously, we characterized a WOX gene from Lilium lancifolium, LlWOX11, positively regulating bulbil formationin, and showed that it bound to the motif of TTAATGAG. However, whether LlWOX11 could bind to other motifs is unclear. In this study, Transcription Factor Centered Yeast One Hybrid (TF-Centered Y1H) was utilized to study the motifs recognized by LlWOX11, and five motifs with seven bases were obtained. In addition to five motifs containing known cis-acting elements: TCAACTC (CAREOSREP1), AGAAAGA (DOFCOREZM/POLLENILELAT52), ACAGTAT (CACTFTPPCA1), we identified that LlWOX11 could bind to two new motifs: TGCGAAA, TCCATCA. We further searched for the core sequences of these motifs by Y1H. Dual-luciferase assay (LUC), Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) were performed to further determine that these motifs were bound by LlWOX11 in the plant. In addition, we found that LlWOX11 inhibited the transcription of LlRR9 by binding to the screened motifs in the promoter and promoted bulbil formation. These findings will help to further reveal the functions of WOX protein and the molecular mechanism of bulbil formation regulated by LlWOX11.
Four new species of Chlorociboria from Yunnan, China
Authors: SU HONGLI , Zhao Qi , Hyde Kevin D. , Luo Le , Chethana K. W. Thilini
Published: June 2024

Chlorociboria, a commonly reported saprobic genus in Chlorociboriaceae, is characterized by discoid, blue-green, olivaceous, yellow or white apothecia, filiform or thin-clavate paraphyses, cylindric-clavate asci, and ascospores that are elliptic to fusiform, or allantoid, hyaline. According to our morphological and phylogenetic studies of nine Chlorociboria collections from southwest China, four new species (C. ailaoense, C. bannaensis, C. laojunense and C. yulongense) are proposed. Chlorociboria ailaoense is identified by its blue to dark blue-green receptacles without tomentum hyphae, along with medially and basally branched paraphyses, and fusiform ascospores. Chlorociboria bannaensis is recognized by light blue receptacles lacking tomentum hyphae, black stipes, a degenerated medullary excipulum, thin-clavate, unbranched paraphyses, inamyloid asci, and ellipsoid ascospores. For C. laojunense, distinctive characters include light blue discs, dark blue-green flanks without tomentum hyphae, asci without croziers, and fusiform ascospores. Chlorociboria yulongense is characterized by olive green to dark discs, white flanks without tomentum hyphae, filiform, branched paraphyses, inamyloid asci, and elliptic to allantoid ascospores. Our phylogenetic analyses, based on the internal transcribed spacer (ITS) and the nuclear ribosomal large subunit (LSU) data of Chlorociboriaceae, strongly support the establishment of the four new species. In addition, we have provided an updated key to distinguish species of Chlorociboria.
Isolation and characterization of a triplet nitrene
Authors: Tan Gengwen , Wang Dongmin , Chen Wang , Chen Haonan , Chen Yizhen , Ye Shengfa
Published: June 2024

Free nitrenes of R–N are short-lived intermediates in a variety of nitrogen-involved transformations. They feature either a singlet ground state or a triplet ground state, depending on the electronic properties of the substituents R. Triplet arylnitrenes typically undergo facile ring expansion to azacycloheptatetraenes, making their isolation in the condensed phase highly challenging. Herein, we present the synthesis, isolation and characterization of an isolable triplet arylnitrene supported by a bulky hydrindacene ligand. The high stability is largely attributed to the sufficient steric hindrance and effective electron delocalization imparted by the supporting ligand. Electron paramagnetic resonance spectroscopy in conjunction with highly correlated wavefunction based ab initio calculations unambiguously demonstrated its triplet ground state with axial zero-field splitting D = 0.92 cm–1 and vanishing rhombicity E/D = 0.002. This work not only presents a long-sought reaction intermediate, but also provides in-depth insights into the reaction mechanisms involving nitrenes.
An unsupervised transfer learning gear fault diagnosis method based on parameter-optimized VMD and residual attention networks
Authors: Ma Jiaocheng , Lv Hongdong , Liu Qin , Yan Lijun
Published: June 2024

Traditional gear intelligent fault diagnosis methods require a large amount of labeled training data. It is challenging to train a high-precision fault diagnosis model due to the issue of insufficient fault data. Transfer learning can reduce the requirement for sufficient labeled data. When the data from the source and target domains differ significantly, the accuracy of the current transfer learning-based fault diagnosis techniques is poor. To deal with this problem, a deep transfer learning gear fault diagnosis method is presented. Firstly, a variational mode decomposition (VMD) and gramian angular field (GAF)-based data preprocessing technique is suggested to denoise the signal and convert the one-dimensional signal into two-dimensional images. Next, this paper proposes an improved residual attention convolutional neural network (IRACNN) to extract the signal's multi-scale spatial features, thereby improving the network's capability to extract gear fault features. Finally, this paper suggests a staged transfer training strategy to align the class-level feature distribution. This paper sets up a gear fault test platform in the laboratory to verify the suggested method and demonstrate its superiority.
Effects of early life protein intake on physical and neuropsychological development of preterm infants aged 0-24 month：a longitudinal cohort study of High-Risk Infant
Authors: Xie Qunying , Li Jiamei , Wen Yuqi , Song Yanyan , Hu Yan
Published: June 2024

Purpose: To investigated whether high protein intake in early life contribute to physical and mental development in preterm infants aged 0-24 months, then analyzed the association between protein intake and incidence of obesity.
Methods: A total of 174 preterm infants were recruited for a cohort study related to Guangzhou High-Risk Infant, and were divided into a high protein intake group (HP, n=21) and a common protein intake group (LP, n=153) based on their average protein intake at the age of 0~6 months. Body weight, body length, and BMI and their Z-scores was calculated, and the Gesell development and infant neuropsychological examination screening were used to assess child mental development. Multivariate Linear regression models were used to assess the correlation between protein intake and physical development, and the multivariate logistic regression was used to analyze the association between protein intake and the level of mental development and the incidence of obesity.
Results: The average protein intake in HP and LP group were 3.76±0.62g/kg/day and 1.67±0.49g/kg/day, respectively. The body weight, body length, BMI, and their Z-scores in the HP group was significantly lower than LP group during aged 6 to18 months. There a negatively correlation between protein intake and physical development level at aged 6~12 months. No significant association was found between protein intake and the mental development and the incidence of obesity.
Conclusions: A higher protein intake in early life (0~6th month) did not contribute to the development of physical and neuropsychological and have no effect on the incidence of obesity and Neuropsychological development in preterm infants aged 0-24 months.
Polydopamine(PDA)-Coated Diselenide-Bridged Mesoporous Silica-based Nanoplatform for Neuroprotection by Reducing Oxidative Stress and Targeting Neuroinflammation in Intracerebral Hemorrhage
Authors: Zhou Fangfang , He Yongju , Zhang Meiru , Gong Xiyu , Liu Xiaoxuan , Tu Ranran , Yang Binbin
Published: June 2024

Oxidative stress (OS) and neuroinflammation are critical pathological processes in secondary brain injury (SBI) after intracerebral hemorrhage(ICH), and their intimate interactions initiate and aggravate brain damage. Thus, targeting oxidative stress and neuroinflammation could be a promising therapeutic strategy for ICH treatment. Here, we report a high-performance platform using polydopamine (PDA)-coated diselenide bridged mesoporous silica nanoparticle (PDA-DSeMSN) as a smart ROS scavenger and ROS-responsive drug delivery system. Caffeic acid phenethyl ester (CAPE) was blocked in the pore of DSeMSN by covering the pore with PDA as a gatekeeper. PDA-DSeMSN @CAPE maintained high stability and underwent reactive oxygen species (ROS)-responsive degradation and drug release. The intelligent nanomaterial effectively eliminated ROS, promoted M1 to M2 microglial conversion and suppressed neuroinflammation in vitro and in vivo. Importantly, intravenous administration of PDA-DSeMSN@CAPE specifically accumulated in perihematomal sites and demonstrated robust neuroprotection in an ICH mouse model with high biological safety. Taking together, the synergistic effect of ROS-responsive drug delivery ability and ROS scavenging ability of PDA-DSeMSN makes it a powerful drug delivery platform and provided new considerations into the therapeutic action to improve ICH-induce brain injury.
Enhanced Influence of Indian Ocean Dipole on Sri Lanka Rainfall in Recent Decades
Authors: Kajakokulan Pathmarasa , Jayawardena Shiromani , Attada Raju
Published: June 2024

The Indian Ocean Dipole (IOD) represents a significant driver of ocean-atmosphere coupled phenomena over the equatorial Indian Ocean (IO), exerting a strong influence on the inter-annual rainfall variability of Sri Lanka. However, the nature of the relationship between the IOD and Sri Lanka rainfall has undergone secular fluctuations over the past decades. Consequently, our study provides an in-depth analysis of the evolving relationship between the IOD and rainfall over the wet zone of Sri Lanka during the IOD peak fall, utilising reanalysis datasets from 1940 to 2020. It has been demonstrated that there is a weak and insignificant correlation between the IOD and rainfall over the wet zone of Sri Lanka during the pre-1980s. On the other hand, a strong and significant correlation was observed during the post-1980s. Furthermore, we found that positive IOD warming was higher in the post-1980s compared to the pre-1980s. This was attributed to strong walker circulation and, as a result, strong moisture convergence and convection over the western IO, which led to an enhanced relationship between rainfall over the wet zone of Sri Lanka and IOD. Thus, this study highlights how ocean-atmosphere changes in the tropical IO have contributed to strengthening the IOD- Sri Lanka rainfall relationship over recent decades and could be valuable for seasonal prediction in Sri Lanka.
Evaluating Mechanical Benefit of Wedge Osteotomies in Endoscopic Surgery for Sagittal Synostosis Using Patient-Specific 3D Printed Models
Authors: Taborsky Jakub , Taborska Jana , Sova Pavel , Maratova Klara , Kodytkova Aneta , Benes Vladimir , Liby Petr
Published: June 2024

Purpose: Endoscopically assisted sagittal strip craniotomy with subsequent cranial orthosis is a frequently used surgical approach for non-syndromic sagittal synostosis. Originally, this technique involved a wide sagittal strip craniectomy with bilateral wedge osteotomies. More recent studies suggest omitting wedge osteotomies, achieving similar outcomes. The controversy surrounding wedge osteotomies and our efforts to refine our technique led us to create models and evaluate the mechanical impact of wedge osteotomies.
Methods: We conducted a 3D-print study involving preoperative CT scans of non-syndromic scaphocephaly patients undergoing Minimally Invasive Assisted Remodelation (MEAR) surgery. The sagittal strip collected during surgery underwent thickness measurement, along with a 3-point bending test. These results were used to determine printing parameters for accurately replicating the skull model. Model testing simulated gravitational forces during the postoperative course and assessed lateral expansion under various wedge osteotomy conditions.
Results: The median sagittal strip thickness was 2.00 mm (range 1.35-3.46 mm) and significantly positively correlated (p = 0.037) with the median force (21.05N) of the 3-point bending test. Model testing involving 40 models demonstrated that biparietal wedge osteotomies significantly reduced the force required for lateral bone shift, with a trend up to 5 cm long cuts (p = 0.007). Additional cuts beyond this length or adding the occipital cut did not provide further significant advantage (p = 0.1643; p = 9.6381).
Conclusion: Biparietal wedge osteotomies reduce the force needed for lateral expansion, provide circumstances for accelerated head shape correction, and potentially reduce the duration of cranial orthosis therapy.
SVF cell sheets: A new multicellular material-based strategy for promoting angiogenesis and regeneration in diced cartilage grafts
Authors: Wei Yangchen , Wei Yi , Xie Cong , Li Zhengyang , Li Li , Chen Yan , Wang Yiping , Jia Chiyu , Xie Hongju , Liao Junlin
Published: June 2024

Autologous diced cartilage, while biocompatible and easy to shape, is limited in clinical application due to its high resorption rate and challenges in establishing timely and effective neovascularization post-surgery. In this study, we produced SVF cell sheets from adipose-derived stromal vascular fraction (SVF) via enzymatic digestion, employing a temperature-sensitive culture system. Our in vivo and in vitro experiments validated that SVF cell sheets, when wrapped around granular cartilage, exhibited a notable promotion of cartilage regeneration and mitigated granular cartilage resorption in a rabbit diced cartilage graft model. Our findings demonstrate that SVF cell sheets facilitated effective neovascularization and timely cartilage block formation by secreting VEGF and Ang-1 while also suppressing the expression of pyroptotic proteins like NLRP3, Caspase1, and GSDMD. As a biofilm, derived from a multicellular source, SVF cell sheets hold promise in promoting neovascularization and cartilage regeneration in diced cartilage grafts while also preventing chondrocyte pyroptosis, presenting a potential novel approach for autologous diced cartilage transplantation.
WITHDRAWN: Pregnant Women’s Knowledge and Beliefs regarding the Affect of Obesity on the Continuation of Breast Feeding
Authors: Alotaibi Jazi Shaydied , AlAnazy Wdad , Mahmoud Fatma zaghloul
Published: June 2024

Background: Assessing the knowledge and beliefs of pregnant women regarding the effect of obesity on breastfeeding continuation is crucial in understanding potential barriers to successful breastfeeding amongst this demographic.
Aim: The current study aimed to evaluate pregnant women's knowledge and beliefs about the effect of obesity on breastfeeding continuation.
Design: A descriptive cross-sectional design was adopted.
Sample: A purposive sample of 550 pregnant women was recruited based on certain inclusion criteria.
Setting: The study was carried out within second cluster hospitals in the Riyadh region.
Tools: The tools utilized to collect the necessary data were; a Structured Interviewing questionnaire tool, Structured obesity knowledge tool and Likert Belief Scale regarding the effect of obesity on breast feeding continuation.
Result: The mean age was 29.47 ± 8.6years. About 57.8% of the study sample had an unsatisfactory level of knowledge about the effect of obesity on breast feeding continuation in comparison with 42.2% of them who had a satisfactory level of knowledge. While 87.6% of the study sample had positive beliefs regarding the effect of obesity on breast feeding continuation as compared to 12.4% who had negative beliefs. Amongst the study sample no statistically significant relationship was found between the belief and knowledge categories regarding the effect of obesity on breastfeeding continuation (P < 0.05).
Conclusion: The pregnant women had an unsatisfactory level of knowledge and positive beliefs regarding the effect of obesity on breast feeding continuation.
Recommendation: develop targeted educational interventions to address misconceptions amongst this target group.
Mechanism of action of resveratrol affecting the biological function of breast cancer through the glycolytic pathway
Authors: Gao Yu , Wang Yaoyao , Wang Baodi , Hu Qunying , Jiang Jirui , Feng Bo , Gao Xiuli , Liu Likun , Zhu Wenbin , Yue Liling
Published: June 2024

Phosphoglycerate kinase 1 (PGK1) plays a crucial role in the glycolytic pathway and its overexpression has a negative impact on tumor development and prognosis. Resveratrol, a natural polyphenolic compound, has gained significant attention in recent years due to its anti-inflammatory, antioxidant, and anti-tumor properties. However, the mechanism by which resveratrol inhibits breast cancer growth, invasion, and metastasis through the PGK1 glycolytic pathway is still not fully understood. Therefore, this study aimed to investigate the inhibitory effects of resveratrol on breast cancer cell proliferation and invasive migration, as well as its ability to promote apoptosis in vitro. Additionally, the study examined the inhibitory effects of resveratrol on the growth of mouse breast cancer graft tumors in vivo. The findings revealed that resveratrol exerted oncogenic effects both in vivo and in vitro. In our study, we provided additional evidence to support the role of resveratrol in breast cancer treatment. Specifically, We found that resveratrol effectively reduced the expression of PGK1 in BT-549 cells. This reduction is achieved by regulating an important transcription factor c-Myc. As a result, the cellular glycolytic pathway is blocked, leading to the inhibition of malignant biological behavior in breast cancer cells. Our findings suggest that targeting the PGK1 glycolytic pathway could be a promising approach for resveratrol-based treatment of breast cancer.
United Predictability of CT radiomics on invasive pathological features in clinical stage IA-IIA non-small cell lung cancer: a double-center study
Authors: Zhao Fengnian , Jiang Wang , Wang Xiaoxue , Zhao Yunqing , yan Qingna , Li Dong , Zhou Guiming
Published: June 2024

Objectives
Limited surgery has received increasing attention to minimize damage and preserve more functional lung tissue. However, invasive pathological features including occult lymph node metastasis, visceral pleural invasion, lymphovascular invasion and tumor spread through air spaces may become risk factors for prognosis after limited surgery. The aim of this study was to unitedly predict these invasive pathological features based on computed tomography (CT) radiomics in patients with early stage non-small cell lung cancer (NSCLC).
Methods
From January 2016 to February 2023, 910 patients with clinical stage IA-IIA NSCLC underwent resection and were divided into training and validation group based on different institution. Radiomics features were extracted by the PyRadiomics software after tumor lesion segmentation and screened by spearman correlation analysis, minimum redundancy maximum relevance and the least absolute shrinkage and selection operator regression analysis. Univariate analysis followed by multivariable logistic regression were performed to estimate the independent predictors. A predictive model was established with visual nomogram and external validation, and evaluated in terms of area under the receiver operating characteristic curve (AUC).
Results
225 patients had invasive pathological features (33.2%), and four independent predictors were identified: larger consolidation diameter (p = 0.032), pleural attachment (p = 0.013), texture (p < 0.001) and Rad-score (p < 0.001). The combined model showed good calibration with an AUC of 0.815, compared with 0.778 and 0.691 when radiomics or traditional CT features were used alone. For the validation group, the AUC was 0.792, compared with 0.745 and 0.701 in radiomics or traditional CT features model.
Conclusion
Our predictive model can non-invasively assess the risk of invasive pathological features in patients with clinical stage IA-IIA NSCLC, enable surgeons perform more reasonable and individualized treatment choices.
Identification and characterization of mitochondrial autophagy-related genes in osteosarcoma and predicting clinical prognosis
Authors: Zhang Hongliang , Zhang Jingyu , Li Shuang , Liu Jinwei , Zhu Kai , Zhang Hong , Guan Boya , Liu Yancheng
Published: June 2024

Background
Osteosarcoma (OS), with its poor prognosis and risk of metastasis, is the most common primary solid malignant bone tumor. Mitochondrial autophagy has been shown to prevent cancer. Therefore, the aim of this study was to investigate the prognostic genes associated with mitochondrial autophagy in OS.
Methods
The OS related dataset, TARGET-OS, GSE99671 and GSE21257 were downloaded from public database. The differential expression genes (DEGs1) were obtained between OS and normal samples in GSE99671. The single-sample Gene Set Enrichment Analysis (ssGSEA) was used to calculate the scores of 29 mitochondrial autophagy related genes (MARGs) in OS samples, and the samples in TARGET-OS were divided into high-/low-rating groups, so as to obtain the DEGs2 between the two groups. Then the DEGs1 were intersected with DEGs2 to obtain mitochondrial autophagy-related differential genes (MDGs). Subsequently, prognostic genes were screened through multiple steps and risk score was calculated. TARGET-OS was divided into high and low risk groups according to the best cutoff value of risk score. Then, GSEA was performed in two risk groups. Furthermore, the correlation between immune microenvironment and prognostic genes was evaluated.
Results
In all 31 MDGs were obtained via overlap of 3,207 DEGs1 and 622 DEGs2. Then, 5 prognostic genes were screened via multi-step regression analysis, namely KLK2, NRXN1, HES5, OR2W3 and HS3ST4. Kaplan-Meier (K-M) curve showed patients in the high-risk group had shorter survival time and lower survival rate. Further, GSEA results suggested that two risk group were enriched into abc transporter and glycolysis glucose production. Immunoanalysis found that 11 immune cells and 3 immune functions were remarkably different between two groups, such as MDSC and Type 1 T helper cell. HS3ST4 had strongest positive correlation with macrophage, and NRXN1 had strongest negative correlation with memory B cell. The expressions of HAVCR2 and PDCD1LG2 were higher in the low-risk group. Dysfunction had remarkable difference between the two risk groups.
Conclusion
In this study, five prognostic genes associated with mitochondrial 1autophagy were identified and a risk model was constructed, providing a new idea for the diagnosis and treatment of OS.
Extracellular Signal-Regulated Kinase is Activated in Podocytes from Patients with Diabetic Nephropathy
Authors: Yamashiro Aoi , Satoh Yasushi , Oshima Naoki , Endo Shogo
Published: June 2024

In recent decades, the global prevalence of diabetes has provided a warning of chronic complications. Diabetic nephropathy (DN) is a serious complication of both type 1 and type 2 diabetes that affects approximately 35% of diabetic individuals. DN is the main cause of end-stage kidney disease, in which the kidneys can no longer function on their own. Podocytes in the glomerulus play a critical role in regulating glomerular permeability, and podocyte injury is the main cause of DN. Therefore, an increasing number of studies have focused on podocyte injury in DN, and interventions targeting podocyte injury have emerged as potential therapeutic strategies against DN. Extracellular signal-regulated kinase (ERK) is a member of the mitogen-activated protein kinase family that plays critical roles in intracellular signal transduction. In human patients with DN, phosphorylated ERK (pERK), the active form of ERK, is increased in the glomerulus. However, information on the expression of pERK, specifically in podocytes in DN, is limited. Meanwhile, high glucose induces ERK activation in immortalized podocyte cell lines, suggesting the involvement of podocytic ERK in DN. We performed an immunohistochemical study to investigate whether podocytic pERK levels increase in patientswith DN. In comparison to healthy controls, patients with DN showed significantly increased pERK expression levels in cells that were positive for the podocyte-specific marker Wilms’ tumor-1 (DN: 51.3 ± 13.1% vs. Control: 7.3 ± 1.6%, p = 0.0158, t-test, n = 4 for each group). This suggests that ERK activation in podocytes is involved in the pathogenesis of DN.
Phytochemical Study of Some Plant Extracts to Assess their Synergistic Corrosion Inhibition Performance-A Comparative Analysis
Authors: Emmanuel Joseph , Ngasoh Fayen , Bello Abdulhakeem , Anye Vitalis , Onwualu Azikwe
Published: June 2024

This work presents a phytochemical study of selected plants extracts to evaluate their potential synergistic corrosion inhibition performance. It was observed that the dominant phytochemicals derived from ten plant leaves and seeds can be synergies in group to enable better understanding of their inhibitory mechanism, which is a potentially significant gap in knowledge. Based on the results of phytochemical screening using FTIR, GC-MS, VU-VIS, and further analysis of the active and predominant phytochemicals, their reaction complex, inhibitor compatibility and other physical and chemical properties as reported in literature, the plant extracts have been grouped into three Organic Green Corrosion inhibitor (OGCI) formulations. Group 1-Greentreat-1234, Group 2-Greentreat-95627 and Group 3-Greentreat-85. The inhibitor adsorption is via, Pie -bond orbital adsorption, Electrostatic adsorption, Chemisorption and Physisorption as reported in literature. The implication of this study is that OGCI has the potential to control wet corrosion of carbon steel.
Reconsideration of the timing of surgery for intestinal obstruction in patients with relapsed ovarian cancer: a case report
Authors: Weijia Wang , Yahong Gong
Published: June 2024

Background
Intestinal obstruction is common in patients with relapsed ovarian cancer. In these patients, surgical decompression is only indicated if conservative treatment has failed. However, prolonged conservative treatment may increase the risk of perioperative complications. Early surgical intervention should be indicated depending on the patient’s individual characteristics.
Case presentation
We report a case of a 55-year-old woman who underwent exploratory laparotomy for intestinal obstruction and relapsed ovarian cancer. The patients received fifteen days of conservative treatment before the surgery. Severe enterogenic sepsis and hyperkalaemia occurred during intestinal decompression, increasing the risk of perioperative complications. Based on the patient’s history and clinical presentation, early operation should be considered.
Conclusions
For patients with intestinal obstruction related to relapsed ovarian cancer, it is important to have a full understanding of the disease and choose the optimal time of surgical intervention based on the individual situation.
Experimental Study of the Conversion of Polyethylene and Polypropylene to Non-Condensable Gases using a Lab-scale Bubble Column Reactor with Molten Metal Catalysis
Authors: Lee Eunji , Yang Won , Lee Uendo , Lee Yongwoon , Lee Youngjae
Published: June 2024

Sustainable solutions for recycling waste plastics are necessary to replace conventional processing techniques. Plastic pyrolysis is a promising technology for converting waste plastic into useful chemicals. This study aims to selectively separate and produce hydrogen (H2) via plastic pyrolysis using a molten metal catalyst. The characteristics of plastic conversion to non-condensable gas under various operating conditions, such as the number of reactors, reaction temperature, and type of molten metal catalyst used, were investigated. Nitrogen (N2) was introduced into a molten metal bubble column reactor containing a metal catalyst, and plastic was then uniformly mixed inside the catalyst through the formed rotating flow. Polyethylene and polypropylene were pyrolyzed at 700–900 ℃. In the single reactor, the fraction of H2 was similar to that reported in previous studies; however, the fraction of light hydrocarbons increased. As the number of reactors increased in the multi-stage reactor, the fraction of CH4 increased to 58%. At elevated reactor temperatures, the fraction of H2 increased to 40% as the decomposition of light hydrocarbons increased. The dominant influence on H2 production was C2H4 decomposition. The present study derived the optimal operating conditions for increasing H2 production during plastic pyrolysis with molten metal catalysts.
NMR–based experimental study on basic physicomechanical properties of saline clay
Authors: He Mingyang , Zhou Fengxi , Wang Liye , Gao Fei
Published: June 2024

In this paper, the effect of salts (Na2SO4,NaCl) on the physicomechanical properties of clay was investigated using the volumetric flask test,Atterberg limits test, and the triaxial shear test, based on the microscopic perspective of pore scale analysis of soil by nuclear magnetic resonance scanning (NMR) technology. The results show that in Na2SO4 solution and NaCl solution, the decrease of double layer thickness and the increase of PH value caused by the increase of cation concentration are the main reasons for the gradual decrease of soil Atterberg limits,adsorbed bound water (ABW) content and shear strength. In addition, when the cation concentration is the same, SO2- 4 has a greater barrier effect like semi-permeable film than Cl−, which makes the double layer thickness of Na2SO4 solution thinner than NaCl solution, the Atterberg limits and ABW content is further reduced; Meanwhile, SO2- 4 has greater intergranular repulsion on the particle surface compared to Cl−, forming a larger pore structure further deteriorating the soil structure and reducing the soil shear strength. It provides more support for studying the effect of salt on the physical and mechanical properties of clay.
Secured Lightweight Encryption using Time-based Singular Value Decomposition (SVD) for Cyber-Physical Systems
Authors: Lavanya G , CH Vasanthkumar , Ragavendar Sanjay
Published: June 2024

Cyber-physical Systems (CPS) represent a pioneering category of IoT systems seamlessly integrating computing, communication, and physical components. These systems interact with the physical world, gather sensor data, interpret information, and regulate processes, facilitating real-time responses and efficient resource utilization across diverse domains such as healthcare, Industry 4.0, autonomous vehicles, and smart grids. However, the implementation of CPS presents multifaceted challenges including security and privacy, in-teroperability, scalability, real-time operation, data management, and energy efficiency. Security, in particular, emerges as a paramount concern due to the vulnerability of CPS to cyberattacks, which can yield severe real-world consequences including physical harm, environmental damage, or financial losses. Protecting sensitive data transmitted within CPS is imperative to thwart unauthorized access, data breaches, and malicious manipulation, especially considering their role in controlling critical infrastructure. Compliance with cybersecuri-ty regulations is essential, necessitating robust security measures throughout the CPS supply chain. Resilience against equipment failure, natural disasters, and cyber threats can be enhanced through redundancy, failover mechanisms, and incident response strategies. Given the computational constraints of CPS devices , the adoption of lightweight encryption algorithms is advocated to ensure data security without overwhelming limited processing power, memory, and energy resources. Effectively managing and upholding CPS necessitates a proactive approach towards implementing stringent security measures and remaining vigilant against evolving threats. In this research, a novel CPS encryption using a Time-based SVD algorithm is developed and tested on various current-generation hardware devices like Node MCU, Arduino, Raspberry Pi, and STM32WLE series controllers used in the Internet of Things (IoT) domain. The proposed security algorithm is compared on various attributes like latency, execution time, and energy consumption for executing the algorithm, and observed from the results that Node MCU outperforms the rest.
Common rules of the organization and architecture of cis-regulatory modules in the human and mouse genomes
Authors: Yuan Sisi , Ni Pengyu , Su Zhengchang
Published: June 2024

Background We have recently predicted comprehensive maps of cis-regulatory modules (CRMs) and constituent transcription factor (TF) binding sites (TFBSs) in the human and mouse genomes, enabling us to investigate the organization and architecture of the CRMs in both genomes.Results We reveal common rules of the organization and architecture of CRMs in the genomes. 1) The numbers and lengths of CRMs and genes on chromosomes are correlated with the sizes of chromosomes. 2) CRMs are unevenly but correlatedly distributed with genes along chromosomes. 3) The number of CRMs and genes within topologically associating domains (TADs) shows a stronger correlation than those in non-TAD regions. 4) CRMs are slightly biasedly distributed downstream of their nearest transcription start sites. 5) A considerable portion (~ 7%) of CRMs overlap core promoters, thus, CRMs can be classified into core promoter-containing (CPC) and core promoter-lacking (CPL) categories. 6) Most (> 90%) of core promoters overlap long CRMs, hence, they rarely exist alone. 7) CPC CRMs tend to be longer than CPL CRMs. 8) TFBSs exhibit extensive overlaps, forming longer TFBS islands. 9) TFBS islands comprise less than half of CRMs in length. 10) Spacers between TFBS islands are subject to similar evolutionary constraints as TFBS islands. Inter-TFBS spacers are less likely to overlap DNase I hypersensitive sites and TF footprints than TFBS islands, suggesting that they may play roles in transcriptional regulation other than direct TF binding.Conclusions The rules governing the organization and architecture of CRMs in the human and mouse genomes are highly conserved.