bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening for resistance to four fungal diseases and associated genomic regions in a snap bean diversity panel
Authors: Campa Ana , Geffroy Valérie , Bitocchi Elena , Noly Alicia , Papa Roberto , Ferreira Juan José
Published: June 2024

Anthracnose, white mold, powdery mildew, and root rot caused by Colletotrichum lindemuthianum, Scletorinia sclerotiorum, Erysiphe spp., and Pythium ultimum, respectively, are among the most frequent diseases that cause significant production losses worldwide in common bean (Phaseolus vulgaris L.). Reactions against these four fungal diseases were investigated under controlled conditions using a diversity panel of 311 bean lines for snap consumption (Snap bean Panel). The genomic regions involved in these resistance responses were identified based on a genome-wide association study conducted with 16,242 SNP markers. The highest number of resistant lines was observed against the three C. lindemuthianum isolates evaluated: 156 lines were resistant to CL124 isolate, 146 lines resistant to CL18, and 109 lines were resistant to C531 isolate. Two well-known anthracnose resistance clusters were identified, the Co-2 on chromosome Pv11 for isolates CL124 and CL18, and the Co-3 on chromosome Pv04 for isolates CL124 and C531. In addition, other lesser-known regions of anthracnose resistance were identified on chromosomes Pv02, Pv06, Pv08, and Pv10. For the white mold isolate tested, 24 resistant lines were identified and the resistance was localized to three different positions on chromosome Pv08. For the powdery mildew local isolate, only 12 resistant lines were identified, and along with the two previous resistance genes on chromosomes Pv04 and Pv11, a new region on chromosome Pv06 was also identified. For root rot caused by Pythium, 31 resistant lines were identified and two main regions were located on chromosomes Pv04 and Pv05. Relevant information for snap bean breeding programs was provided in this work. A total of 20 lines showed resistant or intermediate responses against four or five isolates, which can be suitable for sustainable farm production and could be used as resistance donors. Potential genes and genomic regions to be considered for targeted improvement were provided, including new or less characterized regions that should be validated in future works. Powdery mildew disease was identified as a potential risk for snap bean production and should be considered a main goal in breeding programs.
Interoceptive awareness in a clinical setting: the need to bring interoceptive perspectives into clinical evaluation
Authors: Solano Durán Paola , Morales Juan-Pablo , Huepe David
Published: June 2024

Interoceptive awareness (IA) is crucial to understanding mental health. The Multidimensional Assessment of Interoceptive Awareness (MAIA) scale, available in approximately 30 languages, has gained global recognition for its research applicability. This review highlights the critical importance of integrating IA evaluation in clinical settings, advocating for the MAIA scale’s potential as a screening tool. Through an examination of academic databases, including Scopus, PubMed, Google Scholar, and J-STOR, our analysis spans seven mental health domains: eating disorders (ED), depression, stress, anxiety, autism spectrum disorder (ASD), chronic pain, and suicide ideation (SI). Thirty-eight studies showed links between several dimensions of IA with different disorders. That is, ED was related to Body Trust and Self-Regulation; anxiety to Body Listening, Emotional Awareness, and Self-Regulation; depression to Noticing and Emotional Awareness; ASD to Trusting, Emotional Awareness, and Noticing; chronic pain to Not-Worrying and Self-Regulation; and SI with Trusting. These insights hold profound implications for both clinical practice and mental health research. Integrating IA assessments into standard clinical protocols has the potential to improve our understanding of pathology, enrich patient care, and enhance therapeutic strategies.
Exploratory and confirmatory factor analysis of the sensitivity to punishment and sensitivity to reward questionnaire-revised and its psychometric evaluation among Chinese person with substance use disorder
Authors: Li Shuying , Xie Dongdong , Li Meiting , Che Qingqing , Zhang Jun , Luo Xingwei , Cai Taisheng
Published: June 2024

The Sensitivity to Punishment and Sensitivity to Reward Questionnaire (SPSRQ) is a self-report tool widely used to assess individuals' level of reinforcement sensitivity. Drug addiction is strongly associated with reinforcement sensitivity, but there is a lack of measurement tools to assess reinforcement sensitivity in drug users, necessitating the revision and application of the SPSRQ among drug users. This study recruited 819 drug users (mean age = 34.74; 56.41% female) from five compulsory rehabilitation centers in Hunan Province, China. The applicability of the SPSRQ among person with substance use disorder was assessed by conducting reliability analyses and validity analyses, with retesting performed by 127 individuals after 6 weeks. Exploratory factor analysis for the SPSRQ showed a stable two-factor structure in person with substance use disorder. Confirmatory factor analysis indicated acceptable goodness of fit indexes for the two-factor structure. The SPSRQ also demonstrated good reliability and convergent and discriminant validity evidence. The two-factor structure of the SPSRQ also demonstrated measurement invariance across gender. Further comparative analysis found that the degree of reward sensitivity was higher for males than for females. Generally, the SPSRQ has shown evidence of good reliability and validity in Chinese drug-dependent populations, and it is suitable for research and application with Chinese person with substance use disorder. These findings about the personality traits of people with substance use disorder provide a solid basis for further research.
Assessment of psychological personality traits in transgender groups using the Minnesota multiphasic personality inventory
Authors: Liu Yan , Wang Zhanqiang , Dong Hanwen , Zhang Yueqian , Yang Kebing , Yang Qingyan , Wang Zhiren , Niu Yajuan
Published: June 2024

Objective

To explore the psychological personality characteristics of transgender groups and to determine whether these characteristics differ according to sociodemographic factors.

Methods

This cross-sectional study was conducted between January 2021 and April 2023 at a psychosexual outpatient clinic in a psychiatric hospital in Beijing, China. In total, 481 individuals were included in this study, and demographic information was collected using a self-administered general questionnaire. Psychological personality traits were assessed using the Minnesota Multiphasic Personality Inventory (MMPI).

Results

The mean scores of the assigned male at birth (AMAB) group were significantly higher than those of the male controls for all 10 clinical factors of the MMPI (p < 0.01 or p < 0.001). The scores for both the Masculinity-femininity (Mf) and Depression (D) factors in the AMABs group exceeded the clinical threshold (T > 60) and were the highest and second-highest scores on the entire scale, respectively. Individuals assigned female at birth (AFAB) had significantly higher scores than female controls for Hysteria (Hy), Psychopathic Deviate (Pd), and Hypomania (Ma) (p < 0.05, p < 0.01, and p < 0.001, respectively). There were significant differences in the rates of abnormal values for the various factors of the MMPI (T > 60) according to gender, age, and education (p < 0.05, p < 0.01, and p < 0.001, respectively). Compared to AFABs, AMABs had higher rates of abnormal scores (T > 60) on the Hypochondriasis (Hs), D, Hy, Mf, Paranoia (Pa), Psychasthenia (Pt), Schizophrenia (Sc), and Social Introversion (Si) scales (p < 0.05, p < 0.01, and p < 0.001, respectively). Second, the transgender group aged ≤25 years had higher rates of abnormal scores (T > 60) on the Hs, D, Hy, Pd, Pa, Pt, Sc, and Ma scales (p < 0.05, p < 0.01, and p < 0.001, respectively). Finally, outliers (T > 60) for the Hs, D, Hy, Pd, Pa, Pt, Ma, and Si factors were more prevalent among those with a primary to high school level of education (p < 0.05, p < 0.01, and p < 0.001, respectively).

Conclusion

Assigned male at births may have a variety of psychological vulnerabilities, and there is a need to focus especially on those with a primary to high school level of education, those aged ≤25 years, and transgender females.
The TCO concept in German forensic homicide offenders with schizophrenia spectrum disorders – new findings from a file-based, retrospective cross-sectional study
Authors: Findeis Hannelore , Strauß Maria , Kröber Hans-Ludwig
Published: June 2024

Introduction

There is evidence that there is a small group of people with schizophrenia spectrum disorders who are more likely to commit homicide than those in the general population. However, there is limited knowledge about the psychopathology that leads to homicide in this group. The aim of this study was to examine two commonly used definitions of the Threat/Control-Override (TCO) concept, which aims to identify a certain risk of serious violence in patients with schizophrenia spectrum disorders.

Methods

This is a sub analysis of a file-based, retrospective and exploratory cross-sectional study. All forensic homicide offenders with schizophrenia spectrum disorders who were detained at the Forensic Hospital Berlin as of 31 December 2014 were examined for the occurrence of TCO according to two commonly used definitions.

Results

Of a total of 419 forensic patients with schizophrenia spectrum disorders, 78 committed homicide (18.6%). The forensic homicide offenders with schizophrenia spectrum disorders were characterised by being male, unemployed, single and having committed (attempted) manslaughter. Irrespective of the definition used, the entire TCO complex was present in less than a third of the sample. In both definitions, Threat symptoms were slightly less frequent than Control-Override symptoms. While Threat symptoms occurred less frequently in Stompe et al.’s definition, Control-Override symptoms were the most common. With regard to Kröber’s definition of Threat and Control-Override, the situation is exactly the opposite.

Discussion

Regarding the entire TCO complex, Kröber’s definition seems a little more open and Stompe et al.’s more strict (38.5% vs. 35.9%). Since TCO only occurs in about one third of the subjects in both definitions, neither definition appears to be conclusive. A combination with proportions from both definitions could be a contribution to a future definition of TCO. The present study provides scarcely published primary data on psychopathology in homicide offenders with schizophrenia spectrum disorders, especially on the much discussed TCO concept in two definitions. In order to determine the most useful definition of TCO, to avoid false positives and to identify clear psychopathological risk symptoms, larger samples and comparative studies with offenders and non-offenders should be conducted in the future.
The role of a mindful movement-based program (Movimento Biologico) in health promotion: results of a pre-post intervention study
Authors: Spaccapanico Proietti Stefano , Chiavarini Manuela , Iorio Francesco , Buratta Livia , Pocetta Giancarlo , Carestia Roberta , Gobbetti Camilla , Lupi Chiara , Cosenza Antonio , Sorci Guglielmo , Mazzeschi Claudia , Biscarini Andrea , de Waure Chiara
Published: June 2024

Introduction

Mindful movement is a comprehensive approach that integrates various bodily, emotional and cognitive aspects into physical activity, promoting overall well-being. This study assessed the impact of a mindful movement program, known as Movimento Biologico (MB), on participants psychological well-being (PWB), positive mental health (PMH), sense of coherence (SOC), and interoceptive awareness.

Methods

MB program was conducted for students attending the bachelor’s degree in Kinesiology and Sport Sciences of University of Perugia over 8 weeks (from October 16 to November 27, 2022). Participants were requested to fill in four questionnaires before and after the MB program: (1) 18-item PWB scale; (2) 9-item PMH scale; (3) 13-item SOC scale; (4) 32-item scale for Multidimensional Assessment of Interoceptive Awareness (MAIA). Wilcoxon signed-rank tests were used to assess changes, with significance set at p < 0.05.

Results

Thirty-eight students (mean age 21.2, 60.5% male) participated. Several MAIA subscales, including noticing (p = 0.003), attention management (p = 0.002), emotional awareness (p = 0.007), self-regulation (p < 0.001), body listening (p = 0.001), and trusting (p = 0.001), showed significant improvements. PMH increased significantly (p = 0.015), and there was a significant enhancement in the autonomy subscale of PWB (p = 0.036). SOC and overall PWB also improved, though not significantly.

Conclusion

The MB program significantly improved participants’ positive mental health and interoceptive awareness. This likely resulted from better recognition and management of positive physiological sensations, a stronger link between physical sensations and emotions, enhanced confidence in one’s body, and increased autonomy.
Knowledge, attitudes, and practices of health care professionals regarding dengue fever: need for training and provision of diagnostic equipment in Togo in 2022, a cross-sectional study
Authors: Togan Roméo Mèdéssè , Diallo Amadou Ibra , Zida-Compaoré Wendpouiré Ida Carine , Ba Mouhamadou Faly , Sadio Arnold Junior , Konu Rodion Yao , Bakoubayi Akila Wimima , Tchankoni Martin Kouame , Gnatou Gatibe Yendu-Suglpak , Gbeasor-Komlanvi Fifonsi Adjidossi , Diongue Fatoumata Binetou , Tine Jean Augustin Diégane , Faye Adama , Ekouévi Didier Koumavi
Published: June 2024

Background

Health statistics on dengue are virtually non-existent, despite the fact that the virus is circulating in Togo. This study aimed to assess the knowledge, attitudes, and practices (KAP) of health professionals in the Kara health region.

Methods

A cross-sectional study was conducted from March to June 2022 among healthcare professionals who had worked in the Kara region of northern Togo were selected using an exhaustive recruitment method. Data were collected by trained resident doctors with a face-to-face interview using a standardized, pretested questionnaire based on the WHO 2009 dengue guide. Three multivariate regression models were utilized to investigate factors associated with knowledge, attitudes and, and practices.

Results

A total of 464 respondents (37.1% female), median age 35 years, interquartile range (29–43 years) were included. Only (3.0%) of the participants had received training on dengue fever diagnosis, treatment and prevention in the last 3 years, and 10.3% had dengue rapid diagnostic tests available at their hospital. Half of the respondents (49.1%) had good knowledge of dengue fever, compared with 30.0% who had positive attitudes. Of a total of 256 professionals who had encountered a case of dengue fever in their practice, only 24 (9.4%) had appropriate practices for diagnosing and treating dengue fever. In multivariate analysis, the healthcare professionals who had taken part in ongoing training on dengue fever were more likely to have adequate dengue diagnosis and treatment practice aOR = 8.1; CI 95% = [1.7–36.0].

Conclusion

Strengthening healthcare professionals' dengue-related skills through ongoing training and the provision of dengue diagnostic tests could help improve early detection practices and management of dengue fever in Togo.
A novel multistep approach to standardize the reported risk factors for in-hospital falls: a proof-of-concept study
Authors: La Porta Fabio , Valpiani Giorgia , Lullini Giada , Negro Antonella , Pellicciari Leonardo , Bassi Erika , Caselli Serena , Pecoraro Valentina , Govoni Erika
Published: June 2024

Background

Uncertainty and inconsistency in terminology regarding the risk factors (RFs) for in-hospital falls are present in the literature.

Objective

(1) To perform a literature review to identify the fall RFs among hospitalized adults; (2) to link the found RFs to the corresponding categories of international health classifications to reduce the heterogeneity of their definitions; (3) to perform a meta-analysis on the risk categories to identify the significant RFs; (4) to refine the final list of significant categories to avoid redundancies.

Methods

Four databases were investigated. We included observational studies assessing patients who had experienced in-hospital falls. Two independent reviewers performed the inclusion and extrapolation process and evaluated the methodological quality of the included studies. RFs were grouped into categories according to three health classifications (ICF, ICD-10, and ATC). Meta-analyses were performed to obtain an overall pooled odds ratio for each RF. Finally, protective RFs or redundant RFs across different classifications were excluded.

Results

Thirty-six articles were included in the meta-analysis. One thousand one hundred and eleven RFs were identified; 616 were linked to ICF classification, 450 to ICD-10, and 260 to ATC. The meta-analyses and subsequent refinement of the categories yielded 53 significant RFs. Overall, the initial number of RFs was reduced by about 21 times.

Conclusion

We identified 53 significant RF categories for in-hospital falls. These results provide proof of concept of the feasibility and validity of the proposed methodology. The list of significant RFs can be used as a template to build more accurate measurement instruments to predict in-hospital falls.
Evaluation model of urban tourism competitiveness in the context of sustainable development
Authors: Song Jingya , Xu Bo
Published: June 2024

In the contemporary context marked by globalization and the growing prominence of sustainable development, assessing urban tourism competitiveness has emerged as a crucial research domain. This paper aims to develop a comprehensive model for evaluating city tourism competitiveness, grounded in the principles of sustainable development. The model incorporates factors such as city tourism resources, environmental considerations, economic aspects, and societal factors. This holistic approach seeks to offer valuable insights for the city tourism industry. The study conducts a thorough analysis of current research both domestically and internationally, highlighting gaps and articulating the objectives and significance of the research. Employing a machine learning-based empowerment method, the paper determines the significance of evaluation indices and utilizes the Topsis method for assessing urban tourism competitiveness. Distinguishing itself from traditional evaluation methods, this model integrates the principles of sustainable development throughout the evaluation process, with environmental, social, and economic sustainability serving as pivotal evaluation indicators. Empirical analysis involves the evaluation of tourism competitiveness for select cities, facilitating inter-city comparisons. Results from empirical studies demonstrate the model’s effectiveness in evaluating urban tourism competitiveness, providing targeted developmental recommendations for urban tourism.
Fear of cancer progression and the quality of sexual life of female cancer patients in Romania
Authors: Kállay Éva , Müller-Fabian Andrea , Dégi Csaba László
Published: June 2024

Introduction

As cancer survival rates increase, it has become crucial to pay attention to the long-term quality of life of survivors, including sexual functioning. The quality of sexual life and fear of cancer progression are often unmet needs, significantly impacting cancer patients’ overall quality of life. In this study, we investigate these factors in Romanian female cancer patients and highlight their relationship with mental health and demographic variables.

Methods

This study included 242 Romanian female cancer patients who completed questionnaires assessing sexual functioning (EORTC QLQ-SHQ22), fear of cancer progression (FoP-Q), depression (PHQ-9), and anxiety (GAD-7). We examined these relationships using descriptive, exploratory, and regression analyses.

Results

Around 50% of patients reported impairments in sexual satisfaction and pain during sex. Lower sexual satisfaction increased sexual dysfunction, and heightened fear of cancer progression (FCP) were associated with depression, anxiety, younger age, lower education, rural residence, and unmarried status.

Discussion

This study reveals a complex interplay between sexual health, fear of cancer progression, and psychological well-being among female cancer survivors in Romania. Addressing sexual concerns, providing psychoeducation, promoting coping with the fear of progression, and utilizing interdisciplinary interventions are essential to improving these patients’ overall quality of life. These findings underscore the need for integrated care approaches that consider both physical and psychological dimensions of cancer survivorship.
Is the new mission-oriented R&D policy feasible?: evidence from the national R&D programs in Korea using CVM
Authors: Yoon Jiwoong , Lee Hokyu , Won Yeonghun
Published: June 2024

Introduction

The new mission-oriented national R&D programs, aligned with a transformative paradigm to address societal challenges, face challenges in economic feasibility analysis due to the ongoing development of innovative solutions and the time required for marketization.

Methods

In this study, we employed a Contingent Valuation Method (CVM) to survey 300 Korean households, exploring their willingness to pay for R&D programs hypothetically funded by their taxes. We estimated the mean willingness to pay (WTP) and multiplied it by the total number of households in Korea to project the annual total benefit for each program.

Results

Using metrics like the value-added ratio, marketization success rate, and R&D contribution rate, we assessed the economic value of program benefits. Our analysis indicates a total benefit of KRW 285 billion (KRW 242.5 billion net present value) for a total investment of KRW 990 billion (KRW 505.4 billion net present value), with a benefit-to-cost ratio of 2.08, arming significant economic feasibility.

Discussion

Given the importance of respecting researchers' autonomy, decision-makers may find the CVM a suitable method for validating evidence for new mission-oriented R&D programs across diverse fields.
Revolutionizing respiratory health research: “commercially-available lung-on-a-chip and air-liquid interface systems”
Authors: Park Sohyun , Woo Chang Gyu , Cho Young-Jae
Published: June 2024

Inhalation of ultrafine particles, aerosol contaminants, and cigarette smoke can induce respiratory diseases. As humans are constantly exposed to various exogenous substances, it is crucial to study their impacts on respiratory diseases and airway dysfunction. Recently, organ-on-a-chip technology has been applied in many research studies to understand disease mechanisms, drug screening, and drug testing. The combination of organ-on-a-chip technology and the air-liquid interface (ALI) culture method is emerging as a new platform for realistically mimicking the microenvironment and physiological motions of the human lungs. Breathing motion can be simulated through cyclic stretching, while blood flow can be replicated using channel flow within the chip. The ALI system is critical for mucociliary differentiation, pseudostratified morphology, and epithelial barrier function development. The combination of organ-on-a-chip technology and ALI systems allows the integration of cyclic stretch as a breathing motion and microfluidic channels as circulatory systems. The chip system can also integrate the lung epithelial cells, extracellular matrix, and microstructures, providing microenvironments such as fibroblast, collagen, and immune cells to the epithelial cells. This review discusses chip systems as effective tools for recapitulating human lung environments and how they are applied in biological studies against various pulmonary diseases such as infections or inflammation, fibrosis, and malignancy.
Co-design of health educational materials with people experiencing homelessness and support workers: a scoping review
Authors: Rodriguez Andrea , Shambhunath Shambhunath , Wijesiri Thushani Indumani Devi , Biazus-Dalcin Camila , Mc Goldrick Niall
Published: June 2024

Introduction

People experiencing homelessness are often marginalised and encounter structural barriers when seeking healthcare. Community-based oral health interventions highlighted the need of well-trained practitioners for the successful engagement of service users and behaviour change. However, a lack of adequate information and specific training has been previously reported. The adoption of inclusive approaches, such as co-design, to develop tailored and meaningful health promotion training and educational materials capable of addressing the specific needs of this group is required. Co-design entails active involvement of different groups in research processes that acknowledge participants' needs and expectations. This scoping review aims to identify the available literature on the participation of people experiencing homelessness and/or their support workers in co-designing health and oral health promotion training/educational materials, approaches adopted, and barriers and enablers to develop these materials.

Methods

The Joanna Briggs Institute (JBI) Scoping Review Methodology informed the development of the scoping review. The protocol was registered on the Open Science Framework. Six electronic databases (Medline (OVID), PsychInfo (OVID), Scopus, Web of Science, Applied Social Sciences Index and Abstracts (ASSIA) (ProQuest) and CINHAL) were systematically searched using MeSH terms. An extensive grey literature search, consultation with experts and hand searching of reference lists took place. Records were screened independently and in duplicate using the Rayyan Qatar Computing Research Institute (QCRI) online tool, followed by qualitative content analysis involving descriptive data coding.

Results

Eight studies/materials were included. Key approaches adopted to co-design, enablers and barriers were captured. The enablers were inclusivity, a safe environment for positive participation, empowerment and flexibility, the barriers were difficulty in recruiting and sustaining participation, power differentials, and limited resources.

Conclusion

The evidence in this area is limited. This scoping review provided foundations for further research to examine the impact of different components of the co-design process including the environment in which the co-design process is conducted. Further studies with experimental design and reported using appropriate study design frameworks detailing active components of the co-design process would strengthen the evidence base in this area.
Climate Change is Happening and Humans are Responsible
Authors: Thorne Peter W. , Gillett Nathan P.
Published: June 2024

We know Earth is warming. Clear evidence of warming can be seen in observations of the atmosphere, oceans, ice, and living things. Computer simulations can tell us what the world would have looked like without increases in greenhouse gases and other human influences, and Earth would not have warmed up like we have seen. By comparing the changes scientists have observed to computer simulations of the climate including or excluding various potential causes, scientists can work out what or who is responsible for the climate changes. Again and again, it has been shown that these simulations can only explain observed climate changes when historic emissions of greenhouse gases, principally arising from burning coal, oil, and gas, are included. The best estimate is that all the warming we have observed since 1850–1900 is due to greenhouse gas emissions produced by human activities.
Food system digitalization and power shifts
Authors: Sodano Valeria
Published: June 2024

The article provides new insights into the assessment of food system digitalization by analyzing how the entire process is mainly power-driven rather than the outcome of fair competition among alternative technological patterns. It focuses on the power forces that have accelerated the digital revolution in the food system and how this revolution is enabling certain subjects to exercise old and new forms of power in the economic, political, and geopolitical spheres. The analysis begins with a brief review of food digital technologies and how the existing literature has discussed their possible benefits and risks. It then focuses on the role of agenda power in promoting digitalization and on hegemonic power as the most important form of power produced by digitalization. The aim of the study is to offer a new perspective, based on the analysis of shifts from one form of political power to another, to better analyze the political issues raised by food system digitalization. The results suggest that to resist the negative aspects of digitalization, it is necessary to transform covert conflicts into overt ones and to understand the mechanisms through which the exercise of power blocks the transition from awareness of conflicts to political action.
Quantitative risk assessment model of the presence of porcine epidemic diarrhea and African swine fever viruses in spray-dried porcine plasma
Authors: Sampedro Fernando , Urriola Pedro E. , van de Ligt Jennifer L. G. , Schroeder Declan C. , Shurson Gerald C.
Published: June 2024

Introduction

There are no microbiological regulatory limits for viruses in animal feed and feed ingredients.

Methods

A performance objective (PO) was proposed in this study to manufacture a spray-dried porcine plasma (SDPP) batch absent of any infectious viral particles. The PO levels of −7.0, −7.2, and −7.3 log TCID50/g in SDPP were estimated for three batch sizes (10, 15, and 20 tons).

Results and discussion

A baseline survey on the presence of porcine epidemic diarrhea virus (PEDV) in raw porcine plasma revealed a concentration of −1.0 ± 0.6 log TCID50/mL as calculated using a TCID50-qPCR derived standard curve. The mean African swine fever virus (ASFV) concentration in raw plasma was estimated to be 0.6 log HAD50/mL (0.1–1.4, 95% CI) during a pre-clinical scenario (collected from asymptomatic and undetected viremic pigs). Different processing scenarios (baseline: spray-drying + extended storage) and baseline + ultraviolet (UV) radiation were evaluated to meet the PO levels proposed in this study. The baseline and baseline + UV processing scenarios were >95 and 100% effective in achieving the PO for PEDV by using different batch sizes. For the ASFV in SDPP during a pre-clinical scenario, the PO compliance was 100% for all processing scenarios evaluated. Further research is needed to determine the underlying mechanisms of virus inactivation in feed storage to further advance the implementation of feed safety risk management efforts globally.
Hydrogen sulfide and its role in female reproduction
Authors: Pilsova Aneta , Pilsova Zuzana , Klusackova Barbora , Zelenkova Natalie , Chmelikova Eva , Postlerova Pavla , Sedmikova Marketa
Published: June 2024

Hydrogen sulfide (H2S) is a gaseous signaling molecule produced in the body by three enzymes: cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST). H2S is crucial in various physiological processes associated with female mammalian reproduction. These include estrus cycle, oocyte maturation, oocyte aging, ovulation, embryo transport and early embryo development, the development of the placenta and fetal membranes, pregnancy, and the initiation of labor. Despite the confirmed presence of H2S-producing enzymes in all female reproductive tissues, as described in this review, the exact mechanisms of H2S action in these tissues remain in most cases unclear. Therefore, this review aims to summarize the knowledge about the presence and effects of H2S in these tissues and outline possible signaling pathways that mediate these effects. Understanding these pathways may lead to the development of new therapeutic strategies in the field of women’s health and perinatal medicine.
Relationship between Personal Ethics and Burnout: The Unexpected Influence of Affective Commitment
Authors: Santiago-Torner Carlos , Corral-Marfil José-Antonio , Tarrats-Pons Elisenda
Published: June 2024

Objective: Ethical climates and their influence on emotional health have been the subject of intense debates. However, Personal Ethics as a potential resource that can mitigate Burnout syndrome has gone unnoticed. Therefore, the main objective of this study is to examine the effect of Personal Ethics on the three dimensions that constitute Burnout, considering the moderating influence of Affective Commitment. Design/methodology: A model consisting of three simple moderations is used to solve this question. The sample includes 448 professionals from the Colombian electricity sector with university-qualified education. Findings: Personal Ethics mitigates Emotional Exhaustion and Depersonalization, but it is not related to Personal Realization. Affective Commitment, unexpectedly, has an inverse moderating effect. In other words, as this type of commitment intensifies, the positive impact of Personal Ethics on Burnout and Depersonalization decreases until it disappears. Furthermore, Affective Commitment does not influence the dynamic between Personal Ethics and self-realization. Research limitations/implications: A longitudinal study would strengthen the causal relationships established in this research. Practical implications: Alignment of values between the individual and the organization is crucial. In fact, integration between the organization and its personnel through organic, open and connected structures increases psychological well-being through values linked to benevolence and understanding. Social implications: Employees’ emotional health is transcendental beyond the organizational level, as it has a significant impact on personal and family interactions beyond the workplace. Originality/value: The potential adverse repercussion of Affective Commitment has been barely examined. Additionally, Personal Ethics, when intensified by high Affective Commitment, can lead to extra-role behaviors that transform what is voluntary into a moral imperative. This situation could generate emotional fractures and a decrease in achievement. This perspective, compared to previous research, introduces an innovative element.
Seed Priming with Poly-Gamma-Glutamic Acid (γ-PGA) Improves Rice Germination Performance under Drought Conditions
Authors: Dueñas Conrado , Calvio Cinzia , Slamet-Loedin Inez Hortense , Susanto Untung , Macovei Anca
Published: June 2024

Drought poses a significant threat to global food security, particularly impacting rice cultivation during the germination stage. In this study, a soil-based system that utilizes soil moisture content was used to simulate optimal and stress conditions to assess the effect of the specific seed priming protocols on germination. Eleven rice varieties, representative of indica and japonica subspecies, grown in different ecosystems and having diverse nutrient contents, were treated with water or solutions of either poly-gamma-glutamic acid (γ-PGA) or denatured γ-PGA. Collected data regarding germinability and stress indices revealed different drought sensitivity between japonica and indica subspecies and genotype-specific responses to priming. Particularly, γ-PGA improved germination of highly susceptible indica varieties whereas water soaking was more effective for the moderately sensitive japonica varieties. Integrative analyses evidenced differences between biofortified and non-biofortified rice under γ-PGA treatment, suggesting a possible correlation between γ-PGA efficacy and Zn/Fe seed content. These findings underline that priming strategies should be tailored based on genotype and therefore this factor should be always taken under consideration for future works. The current study provides relevant information for optimizing seed priming techniques to sustain the development of drought-resilient crops as a sustainable strategy to address agricultural resilience and safeguard food security amidst environmental challenges.
Meta-Analyses of Methionine Source Concept Validation Trials in Broilers
Authors: Lemme Andreas , Li Zeyang , Dorigam Juliano
Published: June 2024

While the supplementation of methionine (Met) sources in broiler feeds has been established for several decades, there is debate on the nutritional value of the methionine hydroxy analogue of methionine (MHA) relative to DL-Met. Based on a recommendation suggesting that MHA is 65% as effective as DL-Met, many feeding trials have been conducted to challenge this recommendation. A literature search found 25 publications contributing 95 data sets suitable to compute Hedges’ g effect sizes used in the meta-analysis. The data had very little heterogeneity of almost zero and the small effect sizes of the DL-Met results were not significantly different from MHA. Data were split in various subgroups, finally suggesting that neither broiler strain (Cobb 500, Ross 308), diet type (corn, wheat based), origin of data (peer-reviewed, grey literature), nor MHA product (MHA-free acid, MHA-calcium salt) impacted the outcome of the meta-analysis. Moreover, distinguishing data in groups with dietary Met+Cysteine (Cys) levels below, at, or above requirement demonstrated that there was no interaction with general Met+Cys supply. It is therefore concluded that MHA products can be replaced by DL-Met in a weight-to-weight ratio of 100:65 in any production condition without compromising broiler performance
Sowing Performance of the Seeder Drill for the 2BYG-220 Type Combined Rapeseed Planter under Vibration Conditions
Authors: Zhang Le , Liu Yafu , Wu Mingliang , Wu Zhili
Published: June 2024

The direct sowing of rapeseed requires shallow tillage for stubble removal, which can cause significant vibrations that impact the seed metering device, thereby affecting the quality of seeding. This study focuses on a double-row hole-wheel-type seed metering device on the 2BYG-220 type combined rapeseed planter. Initially, vibrations experienced by the seed drill during field operations were measured and analyzed, revealing that the vibration frequencies during field operations predominantly ranged between 0 and 25 Hz. Consequently, an eccentric wheel–return-spring-type vibration seed metering test rig was designed, manufactured, and set up. By swapping out the eccentric wheel on the test rig, the amplitude was mainly concentrated within 3 mm. The test results indicate that amplitude had a minimal impact on the performance of the double-row hole-wheel-type rapeseed seed metering device, whereas vibration frequency had a more significant effect. When the vibration frequency was between 0 and 10 Hz, the seed metering device maintained a stable output between 7.6 and 8.2 g/min, with minimal impact from vibrations: the coefficient of variation for seeding uniformity ranged between 35.81% and 44.58%, indicating stability and good uniformity. However, when the vibration frequency ranged from 10 to 24 Hz, the output of the seed metering device decreased rapidly and exhibited a linear relationship with frequency changes, with a determination coefficient (R2) of 0.92376. The coefficient of variation for seeding uniformity increased rapidly and also showed a linear relationship with frequency changes, with a determination coefficient (R2) of 0.87973. Vibrations with frequencies greater than 10 Hz had a considerable impact on the performance of the seed metering device.
The Spatial Distribution Characteristics and Possible Influencing Factors of Landslide Disasters in the Zhaotong Area, Yunnan Province of China
Authors: Wang Wantong , Ma Siyuan , Yan Wujian , Yuan Renmao
Published: June 2024

The Zhaotong area in Yunnan Province stands out as one of the most susceptible areas to landslide disasters. The landslide susceptibility of the Zhaotong area can be attributed to its steep terrain, fractured rock formations and strong rainfall, compounded by its frequent seismic activity. This study utilized landslide data provided by the Zhaotong City Natural Resources and Planning Bureau and visually interpreted from high-resolution satellite images of Google Earth to establish the landslide database of the Zhaotong area, including 161 landslides and 3646 potential geological disasters. The distribution characteristics and possible influencing factors of landslides within the Zhaotong area were analyzed using the aforementioned data. The results show that the spatial distribution of landslides and potential geological disasters is roughly consistent; the most concentrated landslides occurred at the junction of Yiliang County, Zhaotong City, and Daguan County, indicating the necessity to enhance surveillance of these landslide-prone areas. The relationship of landslide locations and different influencing factors suggests that elevation, slope angle, and distance to rivers are closely related to landslide occurrence. Landslides are more likely to occur in areas with lower elevations with slope angles ranging from 10° to 40° and near river channels.
A Multi-Step Image Pre-Enhancement Strategy for a Fish Feeding Behavior Analysis Using Efficientnet
Authors: Feng Guofu , Kan Xiaojuan , Chen Ming
Published: June 2024

To enhance the accuracy of lightweight CNN classification models in analyzing fish feeding behavior, this paper addresses the image quality issues caused by external environmental factors and lighting conditions, such as low contrast and uneven illumination, by proposing a Multi-step Image Pre-enhancement Strategy (MIPS). This strategy includes three critical steps: initially, images undergo a preliminary processing using the Multi-Scale Retinex with Color Restoration (MSRCR) algorithm, effectively reducing the impact of water surface reflections and enhancing the visual effect of the images; secondly, the Multi-Metric-Driven Contrast Limited Adaptive Histogram Equalization (mdc) technique is applied to further improve image contrast, especially in areas of low contrast, by adjusting the local contrast levels to enhance the clarity of the image details; finally, Unsharp Masking (UM) technology is employed to sharpen the images, emphasizing their edges to increase the clarity of the image details, thereby significantly improving the overall image quality. Experimental results on a custom dataset have confirmed that this pre-enhancement strategy significantly boosts the accuracy of various CNN-based classification models, particularly for lightweight CNN models, and drastically reduces the time required for model training compared to the use of advanced ResNet models. This research provides an effective technical route for improving the accuracy and efficiency of an image-based analysis of fish feeding behavior in complex environments.
Comparative Study of the Phytochemical Profile and Biological Activity of Ajuga reptans L. Leaf and Root Extracts
Authors: Dziki Anna , Malinowska Magdalena Anna , Szopa Agnieszka , Sikora Elżbieta
Published: June 2024

In this work, the phytochemical composition and the biological activity of the ethanolic extracts obtained from Ajuga reptans L. (Lamiaceae) leaves and roots (growing in Lesser Poland Voivodeship, Poland) were compared. The phytochemical composition of the extracts were determined by the high-performance liquid chromatography with diode-array detection (HPLC–DAD) method. The dominant compounds in both extracts were verbacoside, isoverbacoside, 3,4-dihydroxyphenylacetic acid and rosmarinic acid. The antioxidant capacity of the extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and 2,2’-azobis(3-ethylbenzothiazoline-6-sulfonate (ABTS) tests. The Folin–Ciocalteu assay was used to determine the total polyphenolic content. Additionally, enzyme (tyrosinase, collagenase) inhibition tests and metal chelating ability were studied to assess the anti-aging properties of the extracts. Moreover, the A. reptans extracts’ capacity to absorb the whole range of ultraviolet radiation and high-energy visible (HEV) light was evaluated. The skin irritation test (SIT) EpiDerm was applied to evaluate the safety of the bugle extracts. The noteworthy point is that there is a lack of literature on the assessment of A. reptans root extract activity. Our study is the only one that compares the quantitative composition and biological activity of extracts from the root and leaves of A. reptans. The obtained results indicated that both of the extracts exhibit high antioxidant, chelating and photoprotective activity, but the extract from A. reptans roots showed a higher enzyme inhibition effect for mature skin. The A. reptans root extract, similarly to the leaf extract, could be applied as potentially multi-functional, safe and sensitive cosmetic raw materials, especially in anti-aging and anti-pollution cosmetics.
Valorisation of Spent Yeast Fermentation Media through Compositional-Analysis-Directed Supplementation
Authors: Murphy Laura , Lynch Ciara D. , O’Connell David J.
Published: June 2024

Spent fermentation media from bioprocessing represent a significant waste stream, and interest in recycling them as part of the developing circular bioeconomy is growing. The potential to reuse yeast spent culture media (YSM) to feed secondary bacterial fermentations producing recombinant protein was investigated in this study. Elemental and amino acid compositional analysis using inductively coupled plasma mass spectrometry (ICP-MS) and LC-MS/MS identified significant differences in the concentrations of 6 elements and 18/20 amino acids in YSM compared with rich microbiological media (LB). Restoration of levels of magnesium and sodium through addition of their salts and amino acids from tryptone supplementation led to the expression of equivalent titres of recombinant proteins by E. coli (0.275 g/L), compared to that in LB media (0.296 g/L) and BMMY media (0.294 g/L) in shake flask culture. When this supplementation strategy was employed in a bioreactor system, we observed a significant increase in recombinant protein titre using the supplemented YSM (2.29 (±0.02) g/L) over that produced using LB media (1.29 (±0.09) g/L). This study demonstrates through highly sensitive compositional analysis and identification of supplementation strategies the potential to valorise spent media from yeast fermentations that underpin industrial processes of significant scale, creating a circular approach to waste stream management.
A Thorough Insight into the Biofilm Properties of Potential Fish Pathogen Tenacibaculum discolor Strain FMCC B487
Authors: Schoina Eirini , Marchand Laetitia , Zykwinska Agata , Sinquin Corinne , Leroi Françoise , Delbarre-Ladrat Christine , Nychas George-John E.
Published: June 2024

Tenacibaculum discolor develops biofilm in marine aquaculture production tanks and is identified as one of the causative agents of tenacibaculosis, a bacterial disease that causes significant losses in marine aquaculture production. In this study, the biofilm characteristics of T. discolor strain FMCC B487 were evaluated. Cell growth and biofilm formation and development were studied in miniaturized assays to assess the effect of different levels of environmental factors temperature and salinity, as well as the presence of monosaccharides potentially found in aquaculture hatcheries. The ability of the strain to grow and develop strong biofilms in ambient to high temperatures and at salinities above 20 g/L was shown. Mannose was the monosaccharide with the most prominent impact on the T. discolor strain FMCC B487 biofilm. The composition of planktonic cell extract, biofilm extracts, and extracellular polymeric substances (EPS) produced by T. discolor strain FMCC B487 were investigated by means of colorimetric and fluorometric assays as well as analyses by electrophoresis, gas chromatography, and high-performance size-exclusion chromatography coupled with a multiangle light scattering detector, revealing the dominance of proteins and lipids and the absence of high-molecular-weight polysaccharides. This information may serve as a basis for considering anti-biofilm strategies against the pathogen T. discolor.