bluuebunny/crossref_metadata_2025 huggingface dataset:
Fruit Quality and Yield of Three Highbush Blueberry (Vaccinium corymbosum L.) Cultivars Grown in Two Planting Systems under Different Protected Environments
Authors: Smrke Tina , Veberic Robert , Hudina Metka , Zitko Vid , Ferlan Mitja , Jakopic Jerneja
Published: December 2021

Due to the increasing interest in highbush blueberry (Vaccinium corymbosum L.) among consumers, together with the problems of climate change and specific substrate requirements, a novel approach to intensive blueberry production is required. Here, ‘Duke’, ‘Aurora’, and ‘Brigitta’ blueberry cultivars were planted under the protective environments of a high tunnel and black hail net, each using ridge and pot planting systems. The high tunnel increased the maximal air temperature on average by 7.2 °C compared to the hail net. For all three cultivars, harvest began 6 to 18 days earlier under the high tunnel than under the hail net; however, lower yields and individual phenolics contents were obtained for the fruit. In ‘Aurora’ and ‘Brigitta’, environmental conditions under the high tunnel also reduced plant volume and fruit sugar/organic acid ratio. Growing blueberry plants in 60 L pots had no negative effects on plant volume and fruit ripening time, yield, firmness, color, and chemical composition. This study represents the first to compare highbush blueberry grown under the high tunnel and hail net protective environments using ridge and pot planting systems across three different cultivars. Here, we can conclude that optimal highbush blueberry production of ‘Duke’, ‘Aurora’, and ‘Brigitta’ under the climate conditions of the study provides earlier ripening times under the high tunnel. However, according to fruit yield and quality, all three cultivars benefit from the hail net over the high tunnel, while ‘Duke’ and ‘Brigitta’ also benefit in particular from the hail net combined with growth in pots.
Is a Forensic Cohabitation Program Recovery-Oriented? A Logic Model Analysis
Authors: Chen Shu-Ping , Chang Wen-Pin , Fleet Bryan , Rai Santoch , Panteluk Steve , Choy Alberto , Hunter DeAnn
Published: December 2021

Background. Recovery orientation is a movement in mental health practice. Although general mental health services have taken the lead in promoting recovery, forensic psychiatric systems have lagged behind because of the need to reconcile recovery principles with the complexities of legal mandates. Advocating recovery and making systemic changes can be challenging because they require seeking a balance between the competing duties to the patient and the public. This paper used a logic model framework to demonstrate a cohabitation program that placed a woman and her newborn infant in a secure forensic rehabilitation unit, and analyzed the key assumptions of recovery upon which it was based. Methods. This was a qualitative program evaluation. Data collection involved individual interviews with the woman, the infant’s father, five primary healthcare providers, and five system administrators, and 11 focus groups with unit staff and other patients. Content analysis was used to guide the data analysis and develop the critical components of the program logic model. Results. A logic model that consists of input (team building, program planning, staff and patient preparation, resource management), output (logistic activities, risk management, mental healthcare, staff/other patient support, discharge preparation), and outcome (individual, provider, system, and society) components was developed. Conclusions. This study demonstrates a recovery-oriented program for a woman cohabitating with her baby in a secure forensic psychiatric rehabilitation unit. The logic model provided a comprehensive understanding of the way the recovery principles, such as shared decision-making, positive risk-taking, informed choices, and relational security, were implemented.
Citation Network Study on the Use of New Technologies in Neurorehabilitation
Authors: Abuín-Porras Vanesa , Martinez-Perez Clara , Romero-Morales Carlos , Cano-de-la-Cuerda Roberto , Martín-Casas Patricia , Palomo-López Patricia , Sánchez-Tena Miguel Ángel
Published: December 2021

New technologies in neurorehabilitation is a wide concept that intends to find solutions for individual and collective needs through technical systems. Analysis through citation networks is used to search scientific literature related to a specific topic. On the one hand, the main countries, institutions, and authors researching this topic have been identified, as well as their evolution over time. On the other hand, the links between the authors, the countries, and the topics under research have been analyzed. The publications analysis was performed through the Web of Science database using the search terms “new technolog*,” “neurorehabilitation,” “physical therapy*,” and “occupational therapy*.” The selected interval of publication was from 1992 to December 2020. The results were analyzed using CitNetExplorer software. After a Web of Science search, a total of 454 publications and 135 citation networks were found, 1992 being the first year of publication. An exponential increase was detected from the year 2009. The largest number was detected in 2020. The main areas are rehabilitation and neurosciences and neurology. The most cited article was from Perry et al. in 2007, with a citation index of 460. The analysis of the top 20 most cited articles shows that most approach the use of robotic devices and brain–computer interface systems. In conclusion, the main theme was found to be the use of robotic devices to address neuromuscular rehabilitation goals and brain–computer interfaces and their applications in neurorehabilitation.
From Pre- and Probiotics to Post-Biotics: A Narrative Review
Authors: Scarpellini Emidio , Rinninella Emanuele , Basilico Martina , Colomier Esther , Rasetti Carlo , Larussa Tiziana , Santori Pierangelo , Abenavoli Ludovico
Published: December 2021

Background and aims: gut microbiota (GM) is a complex ecosystem containing bacteria, viruses, fungi, and yeasts. It has several functions in the human body ranging from immunomodulation to metabolic. GM derangement is called dysbiosis and is involved in several host diseases. Pre-, probiotics, and symbiotics (PRE-PRO-SYMB) have been extensively developed and studied for GM re-modulation. Herein, we review the literature data regarding the new concept of postbiotics, starting from PRE-PRO-SYMB. Methods: we conducted a search on the main medical databases for original articles, reviews, meta-analyses, randomized clinical trials, and case series using the following keywords and acronyms and their associations: gut microbiota, prebiotics, probiotics, symbiotic, and postbiotics. Results: postbiotics account for PRO components and metabolic products able to beneficially affect host health and GM. The deeper the knowledge about them, the greater their possible uses: the prevention and treatment of atopic, respiratory tract, and inflammatory bowel diseases. Conclusions: better knowledge about postbiotics can be useful for the prevention and treatment of several human body diseases, alone or as an add-on to PRE-PRO-SYMB.
Shaping of Monocyte-Derived Dendritic Cell Development and Function by Environmental Factors in Rheumatoid Arthritis
Authors: Coutant Frédéric
Published: December 2021

Dendritic cells (DC) are heterogeneous cell populations essential for both inducing immunity and maintaining immune tolerance. Chronic inflammatory contexts, such as found in rheumatoid arthritis (RA), severely affect the distribution and the function of DC, contributing to defective tolerance and fueling inflammation. In RA, the synovial fluid of patients is enriched by a subset of DC that derive from monocytes (Mo-DC), which promote deleterious Th17 responses. The characterization of environmental factors in the joint that impact on the development and the fate of human Mo-DC is therefore of great importance in RA. When monocytes leave the blood and infiltrate inflamed synovial tissues, the process of differentiation into Mo-DC can be influenced by interactions with soluble factors such as cytokines, local acidosis and dysregulated synoviocytes. Other molecular factors, such as the citrullination process, can also enhance osteoclast differentiation from Mo-DC, favoring bone damages in RA. Conversely, biotherapies used to control inflammation in RA, modulate also the process of monocyte differentiation into DC. The identification of the environmental mediators that control the differentiation of Mo-DC, as well as the underlying molecular signaling pathways, could constitute a major breakthrough for the development of new therapies in RA.
Endothelial Progenitor Cells and Rheumatoid Arthritis: Response to Endothelial Dysfunction and Clinical Evidences
Authors: Komici Klara , Perna Angelica , Rocca Aldo , Bencivenga Leonardo , Rengo Giuseppe , Guerra Germano
Published: December 2021

Rheumatoid Arthritis (RA) is a chronic autoimmune inflammatory disease characterized by the swelling of multiple joints, pain and stiffness, and accelerated atherosclerosis. Sustained immune response and chronic inflammation, which characterize RA, may induce endothelial activation, damage and dysfunction. An equilibrium between endothelial damage and repair, together with the preservation of endothelial integrity, is of crucial importance for the homeostasis of endothelium. Endothelial Progenitor Cells (EPCs) represent a heterogenous cell population, characterized by the ability to differentiate into mature endothelial cells (ECs), which contribute to vascular homeostasis, neovascularization and endothelial repair. A modification of the number and function of EPCs has been described in numerous chronic inflammatory and auto-immune conditions; however, reports that focus on the number and functions of EPCs in RA are characterized by conflicting results, and discrepancies exist among different studies. In the present review, the authors describe EPCs’ role and response to RA-related endothelial modification, with the aim of illustrating current evidence regarding the level of EPCs and their function in this disease, to summarize EPCs’ role as a biomarker in cardiovascular comorbidities related to RA, and finally, to discuss the modulation of EPCs secondary to RA therapy.
Multigene Profiling of Circulating Tumor Cells (CTCs) for Prognostic Assessment in Treatment-Naïve Metastatic Hormone-Sensitive Prostate Cancer (mHSPC)
Authors: Reichert Zachery R. , Kasputis Tadas , Nallandhighal Srinivas , Abusamra Sophia M. , Kasputis Amy , Haruray Saloni , Wang Yugang , Williams Shamara , Singhal Udit , Alva Ajjai , Cackowski Frank C. , Caram Megan E. V. , Palmbos Phillip L. , Yentz Sarah E. , Smith David C. , Alumkal Joshi J. , Morgan Todd M.
Published: December 2021

The substantial biological heterogeneity of metastatic prostate cancer has hindered the development of personalized therapeutic approaches. Therefore, it is difficult to predict the course of metastatic hormone-sensitive prostate cancer (mHSPC), with some men remaining on first-line androgen deprivation therapy (ADT) for several years while others progress more rapidly. Improving our ability to risk-stratify patients would allow for the optimization of systemic therapies and support the development of stratified prospective clinical trials focused on patients likely to have the greatest potential benefit. Here, we applied a liquid biopsy approach to identify clinically relevant, blood-based prognostic biomarkers in patients with mHSPC. Gene expression indicating the presence of CTCs was greater in CHAARTED high-volume (HV) patients (52% CTChigh) than in low-volume (LV) patients (23% CTChigh; * p = 0.03). HV disease (p = 0.005, q = 0.033) and CTC presence at baseline prior to treatment initiation (p = 0.008, q = 0.033) were found to be independently associated with the risk of nonresponse at 7 months. The pooled gene expression from CTCs of pre-ADT samples found AR, DSG2, KLK3, MDK, and PCA3 as genes predictive of nonresponse. These observations support the utility of liquid biomarker approaches to identify patients with poor initial response. This approach could facilitate more precise treatment intensification in the highest risk patients.
Atrial Fibrillation: Pathogenesis, Predisposing Factors, and Genetics
Authors: Sagris Marios , Vardas Emmanouil P. , Theofilis Panagiotis , Antonopoulos Alexios S. , Oikonomou Evangelos , Tousoulis Dimitris
Published: December 2021

Atrial fibrillation (AF) is the most frequent arrhythmia managed in clinical practice, and it is linked to an increased risk of death, stroke, and peripheral embolism. The Global Burden of Disease shows that the estimated prevalence of AF is up to 33.5 million patients. So far, successful therapeutic techniques have been implemented, with a high health-care cost burden. As a result, identifying modifiable risk factors for AF and suitable preventive measures may play a significant role in enhancing community health and lowering health-care system expenditures. Several mechanisms, including electrical and structural remodeling of atrial tissue, have been proposed to contribute to the development of AF. This review article discusses the predisposing factors in AF including the different pathogenic mechanisms, sedentary lifestyle, and dietary habits, as well as the potential genetic burden.
The Role of Exosomes in Cancer Progression
Authors: Soltész Beáta , Buglyó Gergely , Németh Nikolett , Szilágyi Melinda , Pös Ondrej , Szemes Tomas , Balogh István , Nagy Bálint
Published: December 2021

Early detection, characterization and monitoring of cancer are possible by using extracellular vesicles (EVs) isolated from non-invasively obtained liquid biopsy samples. They play a role in intercellular communication contributing to cell growth, differentiation and survival, thereby affecting the formation of tumor microenvironments and causing metastases. EVs were discovered more than seventy years ago. They have been tested recently as tools of drug delivery to treat cancer. Here we give a brief review on extracellular vesicles, exosomes, microvesicles and apoptotic bodies. Exosomes play an important role by carrying extracellular nucleic acids (DNA, RNA) in cell-to-cell communication causing tumor and metastasis development. We discuss the role of extracellular vesicles in the pathogenesis of cancer and their practical application in the early diagnosis, follow up, and next-generation treatment of cancer patients.
Characterization of GPVI- or GPVI-CD39-Coated Nanoparticles and Their Impact on In Vitro Thrombus Formation
Authors: Nestele Jeremy A. , Rohlfing Anne-Katrin , Dicenta Valerie , Bild Alexander , Eißler Daniela , Emschermann Frederic , Kremser Marcel , Krutzke Konstantin , Schäffer Tilman E. , Borst Oliver , Levi Moran , Korin Netanel , Gawaz Meinrad Paul
Published: December 2021

Traditional antithrombotic agents commonly share a therapy-limiting side effect, as they increase the overall systemic bleeding risk. A novel approach for targeted antithrombotic therapy is nanoparticles. In other therapeutic fields, nanoparticles have enabled site-specific delivery with low levels of toxicity and side effects. Here, we paired nanotechnology with an established dimeric glycoprotein VI-Fc (GPVI-Fc) and a GPVI-CD39 fusion protein, thereby combining site-specific delivery and new antithrombotic drugs. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles, NP-BSA, NP-GPVI and NP-GPVI-CD39 were characterized through electron microscopy, atomic force measurements and flow cytometry. Light transmission aggregometry enabled analysis of platelet aggregation. Thrombus formation was observed through flow chamber experiments. NP-GPVI and NP-GPVI-CD39 displayed a characteristic surface coating pattern. Fluorescence properties were identical amongst all samples. NP-GPVI and NP-GPVI-CD39 significantly impaired platelet aggregation. Thrombus formation was significantly impaired by NP-GPVI and was particularly impaired by NP-GPVI-CD39. The receptor-coated nanoparticles NP-GPVI and the bifunctional molecule NP-GPVI-CD39 demonstrated significant inhibition of in vitro thrombus formation. Consequently, the nanoparticle-mediated antithrombotic effect of GPVI-Fc, as well as GPVI-CD39, and an additive impact of CD39 was confirmed. In conclusion, NP-GPVI and NP-GPVI-CD39 may serve as a promising foundation for a novel therapeutic approach regarding targeted antithrombotic therapy.
The Dynamics of β-Amyloid Proteoforms Accumulation in the Brain of a 5xFAD Mouse Model of Alzheimer’s Disease
Authors: Bugrova Anna E. , Strelnikova Polina A. , Indeykina Maria I. , Kononikhin Alexey S. , Zakharova Natalia V. , Brzhozovskiy Alexander G. , Barykin Evgeny P. , Pekov Stanislav I. , Gavrish Maria S. , Babaev Alexey A. , Kosyreva Anna M. , Morozova Anna Y. , Degterev Daniil A. , Mitkevich Vladimir A. , Popov Igor A. , Makarov Alexander A. , Nikolaev Evgeny N.
Published: December 2021

Alzheimer’s disease (AD) is the leading cause of dementia among the elderly. Neuropathologically, AD is characterized by the deposition of a 39- to 42-amino acid long β-amyloid (Aβ) peptide in the form of senile plaques. Several post-translational modifications (PTMs) in the N-terminal domain have been shown to increase the aggregation and cytotoxicity of Aβ, and specific Aβ proteoforms (e.g., Aβ with isomerized D7 (isoD7-Aβ)) are abundant in the senile plaques of AD patients. Animal models are indispensable tools for the study of disease pathogenesis, as well as preclinical testing. In the presented work, the accumulation dynamics of Aβ proteoforms in the brain of one of the most widely used amyloid-based mouse models (the 5xFAD line) was monitored. Mass spectrometry (MS) approaches, based on ion mobility separation and the characteristic fragment ion formation, were applied. The results indicated a gradual increase in the Aβ fraction of isoD7-Aβ, starting from approximately 8% at 7 months to approximately 30% by 23 months of age. Other specific PTMs, in particular, pyroglutamylation, deamidation, and oxidation, as well as phosphorylation, were also monitored. The results for mice of different ages demonstrated that the accumulation of Aβ proteoforms correlate with the formation of Aβ deposits. Although the mouse model cannot be a complete analogue of the processes occurring in the human brain in AD, and several of the observed parameters differ significantly from human values supposedly due to the limited lifespan of the model animals, this dynamic study provides evidence on at least one of the possible mechanisms that can trigger amyloidosis in AD, i.e., the hypothesis on the relationship between the accumulation of isoD7-Aβ and the progression of AD-like pathology.
Optical Configuration Effect on the Structure and Reactivity of Diastereomers Revealed by Spin Effects and Molecular Dynamics Calculations
Authors: Ageeva Aleksandra A. , Doktorov Alexander B. , Selyutina Olga Yu. , Magin Ilya M. , Ilyina Margarita G. , Borisevich Sophia S. , Rubtsov Ruslan Yu. , Khursan Sergey L. , Stepanov Alexander A. , Vasilevsky Sergey F. , Polyakov Nikolay E. , Leshina Tatyana V.
Published: December 2021

The peculiarities of spin effects in photoinduced electron transfer (ET) in diastereomers of donor-acceptor dyads are considered in order to study the influence of chirality on reactivity. Thus, the spin selectivity—the difference between the enhancement coefficients of chemically induced dynamic nuclear polarization (CIDNP)—of the dyad’s diastereomers reflects the difference in the spin density distribution in its paramagnetic precursors that appears upon UV irradiation. In addition, the CIDNP coefficient itself has demonstrated a high sensitivity to the change of chiral centers: when one center is changed, the hyperpolarization of all polarized nuclei of the molecule is affected. The article analyzes the experimental values of spin selectivity based on CIDNP calculations and molecular dynamic modeling data in order to reveal the effect of optical configuration on the structure and reactivity of diastereomers. In this way, we succeeded in tracing the differences in dyads with L- and D-tryptophan as an electron donor. Since the replacement of L-amino acid with D-analog in specific proteins is believed to be the cause of Alzheimer’s and Parkinson’s diseases, spin effects and molecular dynamic simulation in model dyads can be a useful tool for investigating the nature of this phenomenon.
Green and Highly-Efficient Microwave Synthesis Route for Sulfur/Carbon Composite for Li-S Battery
Authors: Hsu Chun-Han , Chung Cheng-Han , Hsieh Tzu-Hsien , Lin Hong-Ping
Published: December 2021

Multiporous carbons (MPCs) are prepared using ZnO as a hard template and biomass pyrolysis oil as the carbon source. It is shown that the surface area, pore volume, and mesopore/micropore ratio of the as-prepared MPCs can be easily controlled by adjusting the ZnO/oil ratio. Sulfur/MPC (S/MPC) composite is prepared by blending sulfur powder with the as-prepared MPCs followed by microwave heating at three different powers (100 W/200 W/300 W) for 60 s. The unique micro/mesostructure characteristics of the resulting porous carbons not only endow the S/MPC composite with sufficient available space for sulfur storage, but also provide favorable and efficient channels for Li-ions/electrons transportation. When applied as the electrode material in a lithium-ion battery (LIB), the S/MPC composite shows a reversible capacity (about 500 mAh g−1) and a high columbic efficiency (>95%) after 70 cycles. Overall, the method proposed in this study provides a simple and green approach for the rapid production of MPCs and S/MPC composite for high-performance LIBs.
Characterisation of Permanent Deformation Behaviour of Asphalt Mix Based on a Combined Elastic Plastic (CEP) Parameter
Authors: Roy-Chowdhury Abhirup B. , Saleh Mofreh F. , Moyers-Gonzalez Miguel
Published: December 2021

Permanent deformation or rutting is a major mode of failure in Hot Mix Asphalt (HMA) pavements. The binder used in the asphalt mixture plays an important role in the rutting resistance performance of the mixture. Currently, the Superpave rutting parameter and a more advanced test called multiple stress creep and recovery (MSCR) are the most widely used tests for rutting characterisation of asphalt binders. However, they both have their own merits and demerits. This study was undertaken to introduce a combined Elastic-Plastic (CEP) parameter as an additional binder rheological rutting parameters. The study also aimed at investigating the applicability and potential of this parameter to supplement the existing binder rheological parameters to characterise the properties of asphalt binder related to HMA rutting performance. Additionally, the correlations of the binder rheological parameters with the asphalt mix rutting parameters generated by the dynamic creep and the dynamic modulus tests were investigated. For the polymer-modified binders, Styrene-Butadiene-Styrene (SBS) was added to the PG 70-16 binder at two concentration levels (4, and 6% by the mass of the binder). A dense-graded HMA AC 14 was tested in the Dynamic Modulus (DM) and Dynamic Creep (DC) tests for evaluating the rutting performance. The CEP parameter was found to be much more reliable than the traditional G*/sin (δ) and the non-recoverable creep compliance (Jnr) parameters for evaluating the rutting behaviour of polymer modified asphalt binders, evident from better correlations of CEP with the asphalt mix performance. Unlike Jnr, the CEP parameter revealed a wider range of values, which is comparable with asphalt mixture test results.
The Effects of Boric Acid Sugar Bait on Wolbachia Trans-Infected Male Aedes albopictus (ZAP Males®) in Laboratory Conditions
Authors: Aryaprema Vindhya S. , Qualls Whitney A. , Dobson Karen L. , Dobson Stephen L. , Xue Rui-De
Published: December 2021

The field release of Wolbachia trans-infected male mosquitoes, as well as the use of toxic sugar baits, is a novel and promising candidate technique for integrated mosquito management programs. However, the methods of action of the two techniques may not be complementary, because the Wolbachia method releases mosquitoes into the environment expecting a wild population reduction in subsequent generations while the toxic baits are intended to reduce the wild population by killing mosquitoes. This laboratory study was conducted to evaluate the effectiveness of boric acid toxic sugar baits on Wolbachia trans-infected male Aedes albopictus, relative to wild-type Ae. albopictus males. Wolbachia trans-infected (ZAP male®) and the wild-type Ae. albopictus males were exposed separately to 1% boric acid in a 10% sucrose solution in BugDorms. In the control test, the two groups were exposed to 10% sucrose solution without boric acid. Percent mortalities were counted for 24 h, 48 h and 72 h post exposure periods. The results show that 1% boric acid toxic sugar bait can effectively kill ZAP males under laboratory conditions, and the effectiveness was significantly higher after 24 h and 48 h, compared to wild-type male Ae. albopictus. This finding will help in planning and coordinating integrated mosquito management programs, including both Wolbachia trans-infected mosquito releases and the use of toxic sugar baits against Ae. albopictus.
Genome-Wide Selective Signature Analysis Revealed Insecticide Resistance Mechanisms in Cydia pomonella
Authors: Dai Wen-Ting , Li Jin , Ban Li-Ping
Published: December 2021

The codling moth, Cydia pomonella L. (Lepidoptera, Tortricidae), is a serious invasive pest of pome fruits. Currently, C. pomonella management mainly relies on the application of insecticides, which have driven the development of resistance in the insect. Understanding the genetic mechanisms of insecticide resistance is of great significance for developing new pest resistance management techniques and formulating effective resistance management strategies. Using existing genome resequencing data, we performed selective sweep analysis by comparing two resistant strains and one susceptible strain of the insect pest and identified seven genes, among which, two (glycine receptor and glutamate receptor) were under strong insecticide selection, suggesting their functional importance in insecticide resistance. We also found that eight genes including CYP6B2, CYP307a1, 5-hydroxytryptamine receptor, cuticle protein, and acetylcholinesterase, are potentially involved in cross-resistance to azinphos-methyl and deltamethrin. Moreover, among several P450s identified as positively selected genes, CYP6B2, CYP4C1, and CYP4d2 showed the highest expression level in larva compared to other stages tested, and CYP6B2 also showed the highest expression level in midgut, supporting the roles they may play in insecticide metabolism. Our results provide several potential genes that can be studied further to advance understanding of complexity of insecticide resistance mechanisms in C. pomonella.
Perception of Daily Time: Insights from the Fruit Flies
Authors: De Joydeep , Chatterjee Abhishek
Published: December 2021

We create mental maps of the space that surrounds us; our brains also compute time—in particular, the time of day. Visual, thermal, social, and other cues tune the clock-like timekeeper. Consequently, the internal clock synchronizes with the external day-night cycles. In fact, daylength itself varies, causing the change of seasons and forcing our brain clock to accommodate layers of plasticity. However, the core of the clock, i.e., its molecular underpinnings, are highly resistant to perturbations, while the way animals adapt to the daily and annual time shows tremendous biological diversity. How can this be achieved? In this review, we will focus on 75 pairs of clock neurons in the Drosophila brain to understand how a small neural network perceives and responds to the time of the day, and the time of the year.
Performance Analysis of Secure Elements for IoT
Authors: Noseda Mario , Zimmerli Lea , Schläpfer Tobias , Rüst Andreas
Published: December 2021

New protocol stacks provide wireless IPv6 connectivity down to low power embedded IoT devices. From a security point of view, this leads to high exposure of such IoT devices. Consequently, even though they are highly resource-constrained, these IoT devices need to fulfil similar security requirements as conventional computers. The challenge is to leverage well-known cybersecurity techniques for such devices without dramatically increasing power consumption (and therefore reducing battery lifetime) or the cost regarding memory sizes and required processor performance. Various semiconductor vendors have introduced dedicated hardware devices, so-called secure elements that address these cryptographic challenges. Secure elements provide tamper-resistant memory and hardware-accelerated cryptographic computation support. Moreover, they can be used for mutual authentication with peers, ensuring data integrity and confidentiality, and various other security-related use cases. Nevertheless, publicly available performance figures on energy consumption and execution times are scarce. This paper introduces the concept of secure elements and provides a measurement setup for selected individual cryptographic primitives and a Datagram Transport Layer Security (DTLS) handshake over secure Constrained Application Protocol (CoAPs) in a realistic use case. Consequently, the paper presents quantitative results for the performance of five secure elements. Based on these results, we discuss the characteristics of the individual secure elements and supply developers with the information needed to select a suitable secure element for a specific application.
Temporal Relationship of Extraintestinal Manifestations in Inflammatory Bowel Disease
Authors: Fedor Istvan , Zold Eva , Barta Zsolt
Published: December 2021

Objectives: Thus far, few attempted to characterize the temporal onset of extraintestinal manifestations (EIM) in inflammatory bowel diseases (IBD). We sought to determine the time of onset of these findings in a patient cohort with IBD. Methods: We reviewed the electronic health records of 508 IBD patients (303 CD, 205 UC) and summarized general patient characteristics and the temporal relationship and order of presentation of extraintestinal manifestations. Results: CD patients were younger at diagnosis. CD patients with ileocolonic involvement (L3) were younger, and UC patients with pancolitis (E3) were slightly younger at diagnosis. A total of 127 out of 303 (41.91%) CD and 81 out of 205 (39.51%) UC patients had EIMs (p = 0.5898). Some patients presented with EIMs before the diagnosis of IBD (9.45% of Crohn’s disease and 17.28% of ulcerative colitis patients with EIMs, respectively). Of these, seven cases (four in CD and three in UC) were visible by inspection of the patients (either dermatologic or ocular findings). The diagnosis of IBD and extraintestinal symptoms often occurred within a year (22.83% of CD and 16.04% of UC patients). Typically, the diagnosis of the first extraintestinal symptoms happened after the onset of bowel disease (+4.3 (±6.3) years, range: 10 years before to 30 years after in Crohn’s disease and +3.8 (±10) years, range: 24 years before to 30 years after) in ulcerative colitis. UC patients with pancolitis (E3) usually had EIMs earlier in the disease course and displayed EIMs more frequently before IBD diagnosis. Furthermore, patients with pancolitis developed EIMs more frequently than other sub-groups. Conclusion: Extraintestinal manifestations in inflammatory bowel diseases can present at any time, relative to the bowel symptoms. In cases, the presence of a characteristic EIM might be a harbinger of the development of IBD.
Clot Waveform Analysis Demonstrates Low Blood Coagulation Ability in Patients with Idiopathic Thrombocytopenic Purpura
Authors: Wada Hideo , Ichikawa Yuhuko , Ezaki Minoru , Shiraki Katsuya , Moritani Isao , Yamashita Yoshiki , Matsumoto Takeshi , Masuya Masahiro , Tawara Isao , Shimpo Hideto , Shimaoka Motomu
Published: December 2021

Background: Although platelets, which contain large amounts of phospholipids, play an important role in blood coagulation, there is still no routine assay to examine the effects of platelets in blood coagulation. Methods: Hemostatic abnormalities in patients with thrombocytopenia, including those with idiopathic thrombocytopenic purpura (ITP), were examined using clot wave analysis (CWA)–small-amount tissue-factor-induced FIX activation (sTF/FIXa) and thrombin time (TT). Results: Although there were no marked differences in the three parameters of activated partial thromboplastin time (APTT) between normal healthy volunteers and typical patients with ITP, the peak heights of the CWA-sTF/FIXa were markedly low in patients with ITP. The three peak times of the CWA-sTF/FIXa in patients with a platelet count of ≤8.0 × 1010/L were significantly longer than those in patients with a platelet count > 8.0 × 1010/L and the peak heights of the CWA-sTF/FIXa in patients with a platelet count of ≤8.0 × 1010/L were significantly lower than those in patients with >8.0 × 1010/L. The peak heights of the CWA-APTT in patients with ITP were significantly lower than in patients with other types of thrombocytopenia. The three peak heights of the CWA-sTF/FIXa in ITP patients were significantly lower than those in patients with other types of thrombocytopenia. The CWA-TT showed lower peak heights and longer peak times in patients with ITP in comparison to patients with other types of thrombocytopenia. Conclusions: The CWA-sTF/FIXa and CWA-TT results showed that blood coagulation is enhanced by platelets and that the blood coagulation ability in ITP patients was low in comparison to healthy volunteers and patients with other types of thrombocytopenia.
Myocardial Injury after Stroke
Authors: Mihalovic Michal , Tousek Petr
Published: December 2021

The cardiovascular system is markedly affected by stress after stroke. There is a complex interaction between the brain and heart, and the understanding of the mutual effects has increased in recent decades. Stroke is accompanied by pathological disturbances leading to autonomic dysfunction and systemic inflammation, which leads to changes in cardiomyocyte metabolism. Cardiac injury after stroke may lead to serious complications and long-term cardiac problems. Evidence suggests that blood biomarkers and electrocardiogram analyses can be valuable for estimating the severity, prognosis, and therapy strategy in patients after stroke. It is necessary to distinguish whether these abnormalities presenting in stroke patients are caused by coexisting ischemic heart disease or are caused by brain injury directly. Distinguishing the origin can have a great impact on the treatment of patients after acute stroke. In this article, we focus on epidemiology, pathophysiological mechanisms, and the presentation of cardiac changes in patients after stroke.
The Clinical and Immunological Activity Depending on the Presence of Interferon γ in Primary Sjögren’s Syndrome—A Pilot Study
Authors: Sebastian Agata , Madej Marta , Sebastian Maciej , Łuczak Anna , Gajdanowicz Paweł , Zemelka-Wiącek Magdalena , Wiland Piotr
Published: December 2021

The upregulation of IFN pathways and their stimulated genes is associated with primary Sjögren’s syndrome (pSS). The recent studies also indicate the involvement of interferon γ (IFNγ) in the pathogenesis of pSS. The study aimed to assess the clinical and immunological activity depending on the concentration of IFNγ in the peripheral blood in pSS patients. Methods: The study group consisted of patients over 18 years of age with a confirmed diagnosis of pSS. Based on the collected data, disease activity was assessed using the EULAR Sjögren’s syndrome disease activity index (ESSDAI) and the EULAR Sjögren’s syndrome patient reported index (ESSPRI). Results: Among 40 pSS patients, 33 (82%) showed increased levels of IFNγ. The group with positive IFNγ was younger (43 years) than the group with negative IFNγ (57 years) (p < 0.05). In the positive IFNγ group, the time to diagnosis was shorter (p < 0.05). There was a difference in ESSDAI among patients with and without IFNγ (p < 0.05). There were no differences between the groups in ESSPRI and the presence of cryoglobulins, specific anti-SSA, and anti-SSB antibodies and in C3 and C4 hypocomplementemia. RF occurred in both groups with a similar frequency (p = 0.6), but in patients with IFNγ presence, significantly higher RF titers were observed (34.9 vs. 10.5; p < 0.05). Conclusion: In the group of patients with positive IFNγ, the mean value of RF and ESSDAI was higher. This group was also younger than patients with pSS without IFNγ.
First Results from the Prospective German Registry for Childhood Glaucoma: Phenotype–Genotype Association
Authors: Stingl Julia V. , Diederich Stefan , Diel Heidi , Schuster Alexander K. , Wagner Felix M. , Chronopoulos Panagiotis , Aghayeva Fidan , Grehn Franz , Winter Jennifer , Schweiger Susann , Hoffmann Esther M.
Published: December 2021

Childhood glaucoma is a heterogeneous disease and can be associated with various genetic alterations. The aim of this study was to report first results of the phenotype–genotype relationship in a German childhood glaucoma cohort. Forty-nine eyes of 29 children diagnosed with childhood glaucoma were prospectively included in the registry. Besides medical history, non-genetic risk factor anamnesis and examination results, genetic examination report was obtained (23 cases). DNA from peripheral blood or buccal swab was used for molecular genetic analysis using a specific glaucoma gene panel. Primary endpoint was the distribution of causative genetic mutations and associated disorders. Median age was 1.8 (IQR 0.6; 3.8) years, 64% participants were female. Secondary childhood glaucoma (55%) was more common than primary childhood glaucoma (41%). In 14%, parental consanguinity was indicated. A mutation was found in all these cases, which makes consanguinity an important risk factor for genetic causes in childhood glaucoma. CYP1B1 (30%) and TEK (10%) mutations were found in primary childhood glaucoma patients. In secondary childhood glaucoma cases, alterations in CYP1B1 (25%), SOX11 (13%), FOXC1 (13%), GJA8 (13%) and LTBP2 (13%) were detected. Congenital cataract was associated with variants in FYCO1 and CRYBB3 (25% each), and one case of primary megalocornea with a CHRDL1 aberration. Novel variants of causative genetic mutations were found. Distribution of childhood glaucoma types and causative genes was comparable to previous investigated cohorts. This is the first prospective study using standardized forms to determine phenotypes and non-genetic factors in childhood glaucoma with the aim to evaluate their association with genotypes in childhood glaucoma.
Design of Asymmetric Nanofibers-Membranes Based on Polyvinyl Alcohol and Wool-Keratin for Wound Healing Applications
Authors: Sanchez Ramirez Diego O. , Cruz-Maya Iriczalli , Vineis Claudia , Tonetti Cinzia , Varesano Alessio , Guarino Vincenzo
Published: December 2021

The development of asymmetric membranes—i.e., matching two fibrous layers with selected composition and morphological properties to mimic both the epidermis and dermis—currently represents one of the most promising strategies to support skin regeneration during the wound healing process. Herein, a new asymmetric platform fabricated by a sequential electrospinning process was investigated. The top layer comprises cross-linked polyvinylalcohol (PVA) nanofibers (NFs)—from water solution—to replicate the epidermis’s chemical stability and wettability features. Otherwise, the bottom layer is fabricated by integrating PVA with wool-keratin extracted via sulfitolysis. This protein is a biocompatibility polymer with excellent properties for dermis-like structures. Morphological characterization via SEM supported by image analysis showed that the asymmetric membrane exhibited average fiber size—max frequency diameter 450 nm, range 1.40 μm—and porosity suitable for the healing process. FTIR-spectrums confirmed the presence of keratin in the bottom layer and variations of keratin-secondary structures. Compared with pure PVA-NFs, keratin/PVA-NFs showed a significant improvement in cell adhesion in in vitro tests. In perspective, these asymmetric membranes could be promisingly used to confine active species (i.e., antioxidants, antimicrobials) to the bottom layer to support specific cell activities (i.e., proliferation, differentiation) in wound healing applications.
Conductive Scaffolds for Bone Tissue Engineering: Current State and Future Outlook
Authors: Dixon Damion T. , Gomillion Cheryl T.
Published: December 2021

Bone tissue engineering strategies attempt to regenerate bone tissue lost due to injury or disease. Three-dimensional (3D) scaffolds maintain structural integrity and provide support, while improving tissue regeneration through amplified cellular responses between implanted materials and native tissues. Through this, scaffolds that show great osteoinductive abilities as well as desirable mechanical properties have been studied. Recently, scaffolding for engineered bone-like tissues have evolved with the use of conductive materials for increased scaffold bioactivity. These materials make use of several characteristics that have been shown to be useful in tissue engineering applications and combine them in the hope of improved cellular responses through stimulation (i.e., mechanical or electrical). With the addition of conductive materials, these bioactive synthetic bone substitutes could result in improved regeneration outcomes by reducing current factors limiting the effectiveness of existing scaffolding materials. This review seeks to overview the challenges associated with the current state of bone tissue engineering, the need to produce new grafting substitutes, and the promising future that conductive materials present towards alleviating the issues associated with bone repair and regeneration.
Assessment of Technical and Ecological Parameters of a Diesel Engine in the Application of New Samples of Biofuels
Authors: Jablonický Juraj , Feriancová Patrícia , Tulík Juraj , Hujo Ľubomír , Tkáč Zdenko , Kuchar Peter , Tomić Milan , Kaszkowiak Jerzy
Published: December 2021

The technical and environmental parameters of the diesel internal combustion engine using two new samples of biofuels SAMPLE 1 and SAMPLE 2 were evaluated in this paper. SAMPLE 1 and SAMPLE 2 biofuels were tested on a LOMBARDINI LDW 502 internal combustion engine, which was loaded on a dynamometer according to the applicable national and international standards. This method can also be applied to marine engines and contribute to a higher level of marine ecology. The result of the testing was to determine the impact of tested biofuels on the technical parameters engine power and torque and the environmental parameters emissions of smoke, nitrogen oxides, and economy of the internal combustion engine-specific fuel consumption. From the measured data, another parameter was calculated, such as the injected fuel dose and the overall efficiency of the internal combustion engine. The results show that the new samples of SAMPLE 1 and SAMPLE 2 biofuels tested could be a suitable alternative to standard diesel.
CLTS-Net: A More Accurate and Universal Method for the Long-Term Prediction of Significant Wave Height
Authors: Li Shuang , Hao Peng , Yu Chengcheng , Wu Gengkun
Published: December 2021

Significant wave height (SWH) prediction plays an important role in marine engineering areas such as fishery, exploration, power generation, and ocean transportation. For long-term forecasting of a specific location, classical numerical model wave height forecasting methods often require detailed climatic data and incur considerable calculation costs, which are often impractical in emergencies. In addition, how to capture and use the dynamic correlation between multiple variables is also a major research challenge for multivariate SWH prediction. To explore a new method for predicting SWH, this paper proposes a deep neural network model for multivariate time series SWH prediction—namely, CLTS-Net. In this study, the sea surface wind and wave height in the ERA5 dataset of the relevant points P1, P2, and P3 from 2011 to 2018 were used as input information to train the model and evaluate the model’s SWH prediction performance. The results show that the correlation coefficients (R) of CLTS-Net are 0.99 and 0.99, respectively, in the 24 h and 48 h SWH forecasts at point P1 along the coast. Compared with the current mainstream artificial intelligence-based SWH solutions, it is much higher than ANN (0.79, 0.70), RNN (0.82, 0.83), LSTM (0.93, 0.91), and Bi-LSTM (0.95, 0.94). Point P3 is located in the deep sea. In the 24 h and 48 h SWH forecasts, the R of CLTS-Net is 0.97 and 0.98, respectively, which are much higher than ANN (0.71, 0.72), RNN (0.85, 0.78), LSTM (0.85, 0.78), and Bi-LSTM (0.93, 0.93). Especially in the 72 h SWH forecast, when other methods have too large errors and have lost their practical application value, the R of CLTS-Net at P1, P2, and P3 can still reach 0.81, 0.71, and 0.98. The results also show that CLTS-Net can capture the short-term and long-term dependencies of data, so as to accurately predict long-term SWH, and has wide applicability in different sea areas.
Building a Personalized Medicine Infrastructure for Gynecological Oncology Patients in a High-Volume Hospital
Authors: Bizzarri Nicolò , Nero Camilla , Sillano Francesca , Ciccarone Francesca , D’Oria Marika , Cesario Alfredo , Fragomeni Simona Maria , Testa Antonia Carla , Fanfani Francesco , Ferrandina Gabriella , Lorusso Domenica , Fagotti Anna , Scambia Giovanni
Published: December 2021

Gynecological cancers require complex intervention since patients have specific needs to be addressed. Centralization to high-volume centers improves the oncological outcomes of patients with gynecological cancers. Research in gynecological oncology is increasing thanks to modern technologies, from the comprehensive molecular characterization of tumors and individual pathophenotypes. Ongoing studies are focusing on personalizing therapies by integrating information across genomics, proteomics, and metabolomics with the genetic makeup and immune system of the patient. Hence, several challenges must be faced to provide holistic benefit to the patient. Personalized approaches should also recognize the unmet needs of each patient to successfully deliver the promise of personalized care, in a multidisciplinary effort. This may provide the greatest opportunity to improve patients’ outcomes. Starting from a narrative review on gynecological oncology patients’ needs, this article focuses on the experience of building a research and care infrastructure for personalized patient management.
Determinants of Bank M&As in Central and Eastern Europe
Authors: Andrieș Alin Marius , Cazan Sabina , Sprincean Nicu
Published: December 2021

This paper analyzes the determinants of bank mergers and acquisitions (M&As) from a bank-level perspective. The main objective of the study is to identify those mutual characteristics of all banking institutions from Central and Eastern Europe that are prone to be acquired versus acquirer, or national versus cross-border. Using a database of more than 200 M&As transactions between 2000 and 2018 within Central and Eastern Europe, we document the main characteristics that influence the decision of merging, including the size of the bank, profitability, lending activities, liquidity, bank concentration, banking system stability, government effectiveness, regulatory quality, and the level of inflation. Higher effective average tax rate, which is associated with reduced tax avoidance, influences banks in a positive manner to be involved in the M&A process, findings that hold for targeted banks and domestic transactions. Furthermore, the analysis highlights the changes the financial crisis has projected on investors’ behavior.