bluuebunny/crossref_metadata_2025 huggingface dataset:
The Linocuts of Ethel Spowers: A Vision Apart
Authors: Sim Lorraine
Published: August 2020

This essay discusses the colour linocuts of the Melbourne-born artist and illustrator Ethel Spowers. Although Spowers was a key figure in modern art and design in Australia during the 1920s and 1930s, to date her linocuts have received little critical attention and are appraised only briefly and collectively as part and parcel of the Grosvenor School of Modern Art in London, where she studied for several months under the guidance of Iain Macnab and Claude Flight. This essay argues that her modernism provides an important contrast and supplement to accounts of modern everyday life offered by her British and European colleagues at the School, and canonical British and Anglo-American modernism more generally. Rejecting a view of modern life defined in terms of homogenisation, social alienation and adult experience, I discuss how Spowers's rhythmic compositions express choreographies of community and positive affect, and focus on the experience of children.
Dynamic Lifecycle Cost Modeling for Adaptable Design Optimization of Additively Remanufactured Aeroengine Components
Authors: Lawand Lydia , Panarotto Massimo , Andersson Petter , Isaksson Ola , Kokkolaras Michael
Published: August 2020

Additive manufacturing (AM) is being used increasingly for repair and remanufacturing of aeroengine components. This enables the consideration of a design margin approach to satisfy changing requirements, in which component lifespan can be optimized for different lifecycle scenarios. This paradigm requires lifecycle cost (LCC) modeling; however, the LCC models available in the literature consider mostly the manufacturing of a component, not its repair or remanufacturing. There is thus a need for an LCC model that can consider AM for repair/remanufacturing to quantify corresponding costs and benefits. This paper presents a dynamic LCC model that estimates cumulative costs over the in-service phase and a nested design optimization problem formulation that determines the optimal component lifespan range to minimize overall cost while maximizing performance. The developed methodology is demonstrated by means of an aeroengine turbine rear structure.
Differences in the Growth and Physiological Responses of the Leaves of Peucedanum japonicum and Hordeum vulgare Exposed to Salinity
Authors: Liu Liyun , Nakamura Yuki , Taliman Nisar Ahmad , Sabagh Ayman EL , Moghaieb Reda EA , Saneoka Hirofumi
Published: August 2020

Soil salinity has emerged as one of the most prominent threats to modern intensive farming systems, and it has necessitated the cultivation of halophytes to ensure food security and human nutrition. Peucedanum japonicum Thunb. is an edible wild plant with medicinal value that is widely distributed along the Pacific coast of western Japan. However, the adaptive mechanisms of this plant with respect to salt stress tolerance have not yet to be elucidated. The purpose of this study was to compare the physiological responses of P. japonicum to salt stress with those of barley (Hordeum vulgare), which is considered a salinity-tolerant plant. Seedlings of both species at the same height were exposed to different concentrations (0, 50, 75, and 150 mM) of NaCl for 16 days, after which the leaves were analyzed with respect to different physiological parameters. The results revealed a maintenance of leaf growth in P. japonicum compared with that in barley, the growth of which was severely impaired at low concentrations of NaCl (50 and 75 mM). In response to salt stress, a higher suppression of Na+ and Cl− assimilations was observed in P. japonicum than in barley under all NaCl treatments. Moreover, P. japonicum showed a greater ability to maintain leaf K+ and Ca2+ concentrations, whereas barley exhibited a significant reduction in the concentrations of these ions under saline conditions. Thus, the superior salinity tolerance of P. japonicum could be attributed to a more efficient maintenance of ionic balances. Taken together, our results indicate that P. japonicum may be classified as a halophyte, given its superior regulation of K+, Ca2+, SO4−, and sucrose concentrations and lower NO3− concentrations compared with those of barley.
Biological Control of Thielaviopsis paradoxa and Colletotrichum gloeosporioides by the Extracellular Enzymes of Wickerhamomyces anomalus
Authors: Zepeda-Giraud Luis Fernando , Olicón-Hernández Dario Rafael , Pardo Juan Pablo , Villanueva Minerva Georgina Araiza , Guerra-Sánchez Guadalupe
Published: August 2020

An alternative to chemical fungicides in post-harvest diseases are the use of biocontrol agents and their extracellular products against phytopathogens. Two relevant agents in post-harvest infections are Thielaviopsis paradoxa and Colletotrichum gloeosporioides, causing large economic losses in cacao, pineapple, and avocado during storage. In this work, we evaluated the effect of Wickerhamomyces anomalus, an effective biocontrol agent, against these filamentous fungi, focusing on the production of extracellular enzymes and their effect on fungal growth and germination. Moreover, we evaluated the use of inactivated fungal biomass as an inducer in complete (Potato Dextrose Agar and Yeast Peptone Dextrose) and minimal culture media. The antagonistic effect of W anomalus on the growth of both phytopathogens was also studied. The extracellular enzymes in YPD cultures, using T. paradoxa inactivated biomass as the best inducer, were capable of inhibiting the germination of both phytopathogens. In minimal media, only the production of a 30 kDa glucanase with activity against laminarin was observed. The enzyme was effective against the spore germination of T. paradoxa. In post-harvest crop protection tests, growth inhibition of T. paradoxa was observed using the cell-free enzyme extract, which is a promising system to protect cocoa fruits from T. paradoxa during post-harvest.
Impact of Scion and Rootstock Seedling Quality Selection on the Vigor of Watermelon–Interspecific Squash Grafted Seedlings
Authors: Bantis Filippos , Koukounaras Athanasios , Siomos Anastasios S. , Dangitsis Christodoulos
Published: August 2020

Watermelon is mainly grafted onto interspecific squash, and during the season of high demand, seedlings of variable quality are used to cover grafting needs. The objective was to combine watermelon and interspecific squash of different seedling quality categories in order to obtain the optimal combination for the production of high-quality grafted watermelon seedlings. Acceptable seedlings of both species were grouped into quality categories, namely “low”, “optimum”, and “high”. Seedlings of each quality category were combined with each other and grafted seedlings from the nine derived categories were evaluated at two time intervals, at 7 (exit from the healing chamber) and 14 (final product) days after grafting. At both time intervals, watermelon “high” combined with interspecific squash “optimum” exhibited relatively higher shoot length, stem diameter, leaf area, and shoot and root dry weight, as well as shoot dry weight-to-length ratio, which is a quality index. The study shows that watermelon scions should have “high” quality, while interspecific squash rootstocks should have “optimum” quality during grafting for the production of high-quality grafted plants. If possible, “low” to-be-grafted watermelon seedlings should be avoided because the grafted derived seedlings are considered low quality.
Agronomic Evaluation and Chemical Characterization of Sicilian Salvia sclarea L. Accessions
Authors: Tuttolomondo Teresa , Iapichino Giovanni , Licata Mario , Virga Giuseppe , Leto Claudio , La Bella Salvatore
Published: August 2020

Clary sage (Salvia sclarea L.), known for its aromatic and medicinal properties, belongs to the Lamiaceae family. Although the species grows wild throughout Sicily, knowledge of its production and qualitative properties is limited. The aim of this study was to evaluate the agronomic behavior of the species over two years of testing and to characterize the chemical properties of its wild counterparts in order to identify the most promising accessions for cropping or for use in breeding programs. Tests were carried out during 2008, 2009, and 2010. During the first year, the plot was established. Subsequently, the main parameters for bio-agronomic evaluation were taken in 2009 and 2010. Regarding qualitative characterization, essential oils (EO) were extracted from flowering samples of clary sage. The accessions in the study showed satisfactory adaptation capacity to cropping. The accessions examined belong to the “linalyl acetate” (range 36–43%) chemotype. Test results show good potential for Mediterranean cropping systems, helping to increase the range of medicinal and aromatic species in cultivation.
Nematicidal Activity and Phytochemistry of Greek Lamiaceae Species
Authors: Ntalli Nikoletta G. , Ozalexandridou Efstathia X. , Kasiotis Konstantinos M. , Samara Maria , Golfinopoulos Spyros K.
Published: August 2020

Natural pesticides are in the forefront of interest as ecofriendly alternatives to their synthetic ancestors. In the present study, we evaluated the nematicidal activity of seven Greek Lamiaceae species and discerned among principal components for activity according to GC-MS analysis. Care was taken that all botanicals used were easily prepared without employing elaborate procedures and toxic solvents. We established the in vitro EC50 values of the hydrosols of Origanum vulgare L., Mentha piperita L., and Melissa officinalis L. and the water extracts of Origanum vulgare, Τhymus vulgaris L., Thymus citriodorus (Schreb), Rosmarinus officinalis (Spenn), and Ocimum basilicum L. against Meloidogyne javanica (Treub) and Meloidogyne incognita (Kofoid & White). Furthermore, we amended nematode-infested soil with powdered leaves and flowers of O. vulgare to assess for efficacy. According to in vitro studies, the most active botanical preparations against both nematode species was O. vulgare, as regards its hydrosol and water extract. Thymus citriodorus was proved very potent against M. javanica, provoking 100% paralysis at 4 μL/mL after 96 h, but was only nematostatic against M. incognita since the second-stage juveniles (J2s) recovered movement 48 h after immersion in test solutions. Interestingly, O. vulgare was also proved nematicidal in pot bioassays but at test concentrations over 50 g/kg was phytotoxic for tomato plants. According to GC-MS analysis, the principal components sustaining activity of O. vulgare are carvacrol and thymol. The nematicidal activity of O. vulgare seems promising in the forms of essential oil leftovers (i.e., hydrosol), self-prepared water extract that can be of consideration as α “basic substance”, and powder for soil amendment.
Implementing Sustainable Irrigation in Water-Scarce Regions under the Impact of Climate Change
Authors: Nikolaou Georgios , Neocleous Damianos , Christou Anastasis , Kitta Evangelini , Katsoulas Nikolaos
Published: August 2020

The sustainability of irrigated agriculture is threatening due to adverse climate change, given future projections that every one in four people on Earth might be suffering from extreme water scarcity by the year 2025. Pressurized irrigation systems and appropriate irrigation schedules can increase water productivity (i.e., product yield per unit volume of water consumed by the crop) and reduce the evaporative or system loss of water as opposed to traditional surface irrigation methods. However, in water-scarce countries, irrigation management frequently becomes a complex task. Deficit irrigation and the use of non-conventional water resources (e.g., wastewater, brackish groundwater) has been adopted in many cases as part of a climate change mitigation measures to tackle the water poverty issue. Protected cultivation systems such as greenhouses or screenhouses equipped with artificial intelligence systems present another sustainable option for improving water productivity and may help to alleviate water scarcity in these countries. This article presents a comprehensive review of the literature, which deals with sustainable irrigation for open-field and protected cultivation systems under the impact of climatic change in vulnerable areas, including the Mediterranean region.
Dogs Don’t Die Just in Hot Cars—Exertional Heat-Related Illness (Heatstroke) Is a Greater Threat to UK Dogs
Authors: Hall Emily , Carter Anne , O’Neill Dan
Published: August 2020

Heat-related illness will affect increasing numbers of dogs as global temperatures rise unless effective mitigation strategies are implemented. This study aimed to identify the key triggers of heat-related illness in dogs and investigate canine risk factors for the most common triggers in UK dogs. Using the VetCompassTM programme, de-identified electronic patient records of 905,543 dogs under primary veterinary care in 2016 were reviewed to identify 1259 heat-related illness events from 1222 dogs. Exertional heat-related illness was the predominant trigger (74.2% of events), followed by environmental (12.9%) and vehicular confinement (5.2%). Canine and human risk factors appear similar; young male dogs had greater odds of exertional heat-related illness, older dogs and dogs with respiratory compromise had the greatest odds of environmental heat-related illness. Brachycephalic dogs had greater odds of all three types of heat-related illness compared with mesocephalic dogs. The odds of death following vehicular heat-related illness (OR 1.47, p = 0.492) was similar to that of exertional heat-related illness. In the UK, exertional heat-related illness affects more dogs, and kills more dogs, than confinement in a hot vehicle. Campaigns to raise public awareness about heat-related illness in dogs need to highlight that dogs don’t die just in hot cars.
Molecular Evolution and Characterization of Fish Stathmin Genes
Authors: Cao Jun , Cheng Xiuzhu
Published: August 2020

Stathmin is a highly conserved microtubule remodeling protein, involved in many biological processes such as signal transduction, cell proliferation, neurogenesis and so on. However, little evolutional information has been reported about this gene family in fish. In this study, 175 stathmin genes were identified in 27 species of fish. Conserved exon-intron structure and motif distributions were found in each group. Divergence of duplicated genes implied the species’ adaptation to the environment. Functional divergence suggested that the evolution of stathmin is mainly influenced by purifying selection, and some residues may undergo positive selection. Moreover, synteny relationship near the stathmin locus was relatively conserved in some fish. Network analyses also exhibited 74 interactions, implying functional diversity. The expression pattern of some stathmin genes was also investigated under pesticide stress. These will provide useful references for their functional research in the future.
Validation of the Turkey Semen Cryopreservation by Evaluating the Effect of Two Diluents and the Inseminating Doses
Authors: Di Iorio Michele , Rusco Giusy , Iampietro Roberta , Maiuro Lucia , Schiavone Achille , Cerolini Silvia , Iaffaldano Nicolaia
Published: August 2020

This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of three insemination doses (250, 400 and 600 × 106 sperm/hen). Pooled semen samples were diluted with Tselutin or Lake extender which contained 20% of dimethylsulfoxide and 1 mM of Ficoll at final sperm concentration of 3 × 109 sperm/mL. Thereafter, semen was packaged into straws and frozen on liquid nitrogen. The post-thaw sperm quality was evaluated considering motility (computer-aided sperm analysis—CASA system) and membrane integrity (flow cytometry). Significantly higher values of progressive motility and some kinetic parameters in semen frozen with Lake were found. When we compared the extenders in vivo, no significant effects were detected, whilst sperm concentration significantly affected both fertility and hatching rates, with the best results obtained with the sperm concentration of 400 × 106 sperm/hen. From the results obtained, it emerged that the extender type only affected sperm motility characteristics, not the fertilizing ability of frozen-thawed semen, while inseminating dose markedly affected fertility and hatching rates.
Effect of Replacing Dietary Corn with Broken Rice on Goose Growth Performance, Body Size and Bare Skin Color
Authors: Chen Xiaoshuai , Yang Haiming , Xu Lei , Wan Xiaoli , Wang Zhiyue
Published: August 2020

This study investigated the effect of replacing dietary corn with broken rice (BR) on goose growth performance, body size and bare skin color. In total, 240 28-day-old healthy male Yangzhou goslings with similar body weight (BW) were randomly divided into five groups, with six replicates per group and eight geese per replicate. The control group was fed with a corn-soybean meal. The BR25, BR50, BR75 and BR100 groups had 25%, 50%, 75% and 100% of corn replaced with BR, respectively (corresponding to 15.95%, 31.88%, 47.63% and 62.92% of BR in the feed, respectively), each with constant metabolizable energy (ME) to crude protein (CP) ratio (ME/CP). At 28, 42, 56 and 70 d, BW and feed intake for each pen were measured. Blood was collected, and body size and bare skin color were evaluated at 70 d. The results showed that different BR replacement proportions had no effect on BW at 42, 56 or 70 d or on average daily feed intake (ADFI) or average daily gain (ADG) from 28 to 42 d (p > 0.05) but BR50 and BR75 decreased the feed/gain ratio (F/G) from 28 to 42 d (p < 0.05). From 42 to 56 d, BR75 and BR100 geese had a lower ADFI than the control geese (p < 0.05), and BR75 and BR100 geese had a lower F/G than the BR25 geese (p < 0.05). Group BR50, BR75 and BR100 geese had a lower ADFI from 56 to 70 d than the control geese (p < 0.05). From 28 to 70 d, BR50, BR75 and BR100 groups had a lower ADFI (p < 0.05). Interestingly, the control and BR25 groups had a higher flipper score than the BR50, BR75 and BR100 groups (p < 0.05), and the control group had a higher flipper score than the BR25 group (p < 0.05). All BR groups reduced the bill scoring (p < 0.05). Different BR replacement proportions did not negatively affect serum biochemical variable at 70 d (p > 0.05). Overall, under these conditions, BR can totally replace corn in goose diets, and we recommend 75% replacement of corn with BR from 28 to 70 d.
Time-Budget of Horses Reared for Meat Production: Influence of Stocking Density on Behavioural Activities and Subsequent Welfare
Authors: Raspa Federica , Tarantola Martina , Bergero Domenico , Nery Joana , Visconti Alice , Mastrazzo Chiara Maria , Cavallini Damiano , Valvassori Ermenegildo , Valle Emanuela
Published: August 2020

Horses reared for meat production can be kept in intensive breeding farms where they are housed in group pens at high stocking densities. The present study aimed to evaluate whether the expressed behaviours correlated with stocking density, and to compare their time-budget with that of wild-living horses. An ethogram of 13 mutually exclusive behavioural activities was developed. Behavioural observations were performed over a 72 h period on group pens selected on the basis of stocking density and the homogeneity of breed, age, height at the withers, and time since arriving at the farm. Scan sampling (n = 96 scans/horse/day) was used on 22 horses. The mean frequency (%) ± standard deviation (±SD) for each behavioural activity was calculated to obtain the time-budget. The associations between time-budget and stocking density were evaluated using a bivariate analysis. The relationships were analysed by Pearson’s correlation coefficient (r). Our results show that locomotion, playing, and self-grooming positively correlated with a reduction in stocking density, indicating the potential to use these behaviours as positive welfare indicators for young horses kept in group pens. The data also revealed an unusual time-budget, where the main behavioural activity expressed was standing (30.56% ± 6.56%), followed by feeding (30.55% ± 3.59%), lying (27.33% ± 2.05%), and locomotion (4.07% ± 1.06%).
Research on the Applications of Calcium Propionate in Dairy Cows: A Review
Authors: Zhang Fan , Nan Xuemei , Wang Hui , Guo Yuming , Xiong Benhai
Published: August 2020

Calcium propionate is a safe and reliable food and feed additive. It can be metabolized and absorbed by humans and animals as a precursor for glucose synthesis. In addition, calcium propionate provides essential calcium to mammals. In the perinatal period of dairy cows, many cows cannot adjust to the tremendous metabolic, endocrine, and physiological changes, resulting in ketosis and fatty liver due to a negative energy balance (NEB) or milk fever induced by hypocalcemia. On hot weather days, cow feed (TMR or silage) is susceptible to mildew, which produces mycotoxins. These two issues are closely related to dairy health and performance. Perinatal period metabolic disease significantly reduces cow production and increases the elimination rate because it causes major glucose and calcium deficiencies. Feeding a diet contaminated with mycotoxin leads to rumen metabolic disorders, a reduced reproductive rate (increased abortion rate), an increased number of milk somatic cells, and decreased milk production, as well as an increased occurrence of mastitis and hoof disease. Propionic acid is the primary gluconeogenic precursor in dairy cows and one of the safest mold inhibitors. Therefore, calcium propionate, which can be hydrolyzed into propionic acid and Ca2+ in the rumen, may be a good feed additive for alleviating NEB and milk fever in the perinatal period of dairy cows. It can also be used to inhibit TMR or silage deterioration in hot weather and regulate rumen development in calves. This paper reviews the application of calcium propionate in dairy cows.
A Culture-Based ID of Micromycetes on the Wing Membranes of Greater Mouse-Eared Bats (Myotis myotis) from the “Nietoperek” Site (Poland)
Authors: Ogórek Rafał , Kurczaba Klaudia , Cal Magdalena , Apoznański Grzegorz , Kokurewicz Tomasz
Published: August 2020

Bats play important functions in ecosystems and many of them are threatened with extinction. Thus, the monitoring of the health status and prevention of diseases seem to be important aspects of welfare and conservation of these mammals. The main goal of the study was the identification of culturable fungal species colonizing the wing membranes of female greater mouse-eared bat (Myotis myotis) during spring emergence from the “Nietoperek” underground hibernation site by the use of genetic and phenotypic analyses. The study site is situated in Western Poland (52°25′ N, 15°32′ E) and is ranked within the top 10 largest hibernation sites in the European Union. The number of hibernating bats in the winter exceeds 39,000 individuals of 12 species, with M. myotis being the most common one. The wing membranes of M. myotis were sampled using sterile swabs wetted in physiological saline (0.85% NaCl). Potato dextrose agar (PDA) plates were incubated in the dark at 8, 24 and 36 ± 1 °C for 3 up to 42 days. All fungi isolated from the surface of wing membranes were assigned to 17 distinct fungal isolates belonging to 17 fungal species. Penicillium chrysogenum was the most frequently isolated species. Some of these fungal species might have a pathogenic potential for bats and other mammals. However, taking into account habitat preferences and the life cycle of bats, it can be assumed that some fungi were accidentally obtained from the surface of vegetation during early spring activity. Moreover, Pseudogymnoascus destructans (Pd)—the causative agent of the White Nose Syndrome (WNS)—was not found during testing, despite it was found very often in M. myotis during previous studies in this same location.
In Vitro Antibacterial Potential of Salix babylonica Extract against Bacteria that Affect Oncorhynchus mykiss and Oreochromis spp.
Authors: Rangel-López Lenin , Zaragoza-Bastida Adrian , Valladares-Carranza Benjamín , Peláez-Acero Armando , Sosa-Gutiérrez Carolina G. , Hetta Helal F. , Batiha Gaber El-Saber , Alqahtani Ali , Rivero-Perez Nallely
Published: August 2020

Aquaculture development is limited by bacteria associated with several diseases; antibiotics are used for the treatment of these affections, but bacteria have developed resistance to these drugs. It is important to develop effective treatments that allow the production of antibiotic-free food. The aim of the present study is to evaluate the in vitro antibacterial effects of Salix babylonica hydro-alcoholic extract (SbHE) against Aeromonas hydrophila, Listonella anguillarum, Edwarsiella tarda, and Streptococcus iniae, bacteria that affect Oncorhynchus mykiss and Oreochromis spp. production. SbHE was obtained through the maceration technique. Reference strains were used and their sensitivity to antibiotics was determined. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of SbHE were determined. Results showed that three of four evaluated bacteria were multidrug resistant, except S. iniae. SbHE showed antibacterial activity against all bacteria. Results indicate an MIC of 1.56 to 25 mg/mL and an MBC of 3.12 to 100 mg/mL. The greatest inhibitory activity occurred against L. anguillarum obtaining a MIC of 1.56 mg/mL and an MBC of 3.12 mg/mL. Results indicate that SbHE has bactericidal activity against A. hydrophila, L.anguilalurm, and S. iniae as well as bacteriostatic activity against E. tarda and could be an alternative treatment against these bacteria.
Antimicrobial Susceptibility of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli Isolated from Mastitic Dairy Cattle in Ukraine
Authors: Elias Leta , Balasubramanyam Ajay S. , Ayshpur Olena Y. , Mushtuk Iryna U. , Sheremet Nataliya O. , Gumeniuk Volodymyr V. , Musser Jeffrey M. B. , Rogovskyy Artem S.
Published: August 2020

Bovine mastitis is the predominant cause for antimicrobial use on dairy farms and is a major source of economic losses in the dairy industry. In this study, the antimicrobial susceptibility profiles of common mastitis-causing pathogens, Staphylococcus aureus (n = 62), Streptococcus agalactiae (n = 46), and Escherichia coli (n = 129), were determined for dairy cattle with mastitis across 142 Ukrainian farms. The results showed that there were more gentamicin resistant S. aureus isolates (16.95%) identified in this study than previously reported for Ukrainian dairy cattle. Moreover, low levels of amoxicillin susceptibly (13.51%) were observed for St. agalactiae, which contrasted a previous study showing susceptibility levels of >50%. St. agalactiae resistance to tetracycline was observed in 80% of the isolates. Cephalosporin use was most ineffective against E. coli, with 43.27–56% of the isolates exhibiting this resistant trait. Overall, this study performed a preliminary analysis of antimicrobial resistance on mastitis isolates from Ukrainian farms. However, given the limited numbers of the isolates tested in this study and that the publications on antimicrobial resistance in animal husbandry of Ukraine are very few, more extensive investigations are needed to comprehensively examine susceptibility patterns of mastitis-causing pathogens in dairy cattle in Ukraine.
New Insights into Antibacterial Compounds: From Synthesis and Discovery to Molecular Mechanisms of Action
Authors: Leitão Jorge H.
Published: August 2020

The worldwide emergence of microbial resistance to available antibiotics presents a global threat to public health and health systems. This special issue aimed to gather papers describing novel antibiotics, originating form chemical synthesis, repurposing of existent drugs, or from natural sources like plant extracts, herbs and spices. A total of 13 papers were published, covering a wide range of topic, including antimicrobial resistance surveillance studies; synthesis of novel molecules with antimicrobial activities; modification or repurposing of already existing molecules, plant-derived active extracts, and molecules; the effects of antimicrobial therapy on microbiota; and the investigation of novel formulations for human and veterinary uses. After decades of antibiotics discovery decline, antibiotics discovery is boosting. Recent developments of post genomics approaches and bioinformatics tools will most certainly turn the tide in the discovery and development of antimicrobials in this exciting field.
Antioxidants in Down Syndrome: From Preclinical Studies to Clinical Trials
Authors: Rueda Revilla Noemí , Martínez-Cué Carmen
Published: August 2020

There is currently no effective pharmacological therapy to improve the cognitive dysfunction of individuals with Down syndrome (DS). Due to the overexpression of several chromosome 21 genes, cellular and systemic oxidative stress (OS) is one of the most important neuropathological processes that contributes to the cognitive deficits and multiple neuronal alterations in DS. In this condition, OS is an early event that negatively affects brain development, which is also aggravated in later life stages, contributing to neurodegeneration, accelerated aging, and the development of Alzheimer’s disease neuropathology. Thus, therapeutic interventions that reduce OS have been proposed as a promising strategy to avoid neurodegeneration and to improve cognition in DS patients. Several antioxidant molecules have been proven to be effective in preclinical studies; however, clinical trials have failed to show evidence of the efficacy of different antioxidants to improve cognitive deficits in individuals with DS. In this review we summarize preclinical studies of cell cultures and mouse models, as well as clinical studies in which the effect of therapies which reduce oxidative stress and mitochondrial alterations on the cognitive dysfunction associated with DS have been assessed.
Redox-Sensitive Glyoxalase 1 Up-Regulation Is Crucial for Protecting Human Lung Cells from Gold Nanoparticles Toxicity
Authors: Gambelunghe Angela , Giovagnoli Stefano , Di Michele Alessandro , Boncompagni Simona , Dell’Omo Marco , Leopold Kerstin , Iavicoli Ivo , Talesa Vincenzo Nicola , Antognelli Cinzia
Published: August 2020

Gold nanoparticles (AuNPs) are considered nontoxic upon acute exposure, at least when they are equal or above 5 nm size. However, the safeguard mechanisms contributing to maintain cell viability are scarcely explored so far. Here, we investigated the cyto-protective role of Glyoxalase 1 (Glo1), a key enzyme involved in the control of deleterious dicarbonyl stress, in two human cell types of the respiratory tract, after an acute exposure to AuNPs with a main size of 5 nm. We found that the redox sensitive Nrf-2-mediated up-regulation of Glo1 was crucial to protect cells from AuNPs-induced toxicity. However, cells challenged with a pro-inflammatory/pro-oxidative insult become susceptible to the pro-apoptotic effect of AuNPs. Notably, the surviving cells undergo epigenetic changes associated with the onset of a partial epithelial to mesenchymal transition (EMT) process (metastable phenotype), driven by the increase in dicarbonyl stress, consequent to Glo1 inactivation. As a physiological respiratory epithelium is required for the normal respiratory function, the knowledge of the protective mechanisms avoiding or (when challenged) promoting its modification/damage might provide insight into the genesis, and, most importantly, prevention of potential health effects that might occur in subjects exposed to AuNPs, through targeted surveillance programs, at least under specific influencing factors.
Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation
Authors: Coscueta Ezequiel R. , Reis Celso A. , Pintado Manuela
Published: August 2020

Phenylethyl isothiocyanate (PEITC) was reported as a useful antioxidant, anti-inflammatory, and chemopreventive agent. Due to technological and stability issues, it is necessary to be able to extract PEITC from its natural matrix (watercress) through sustainable and scalable methodologies. In this article, we explored, for the first time, the extractive capacity of aqueous micellar systems (AMSs) of two non-ionic surfactants. For this, we compared the AMSs with conventional organic solvents. Furthermore, we developed and optimised a new integral PEITC production and extraction process by a multifactorial experimental design. Finally, we analysed the antioxidant capacity by the oxygen radical absorbance capacity (ORAC) and ABTS methods. As results, the AMSs were able to extract PEITC at the same level as the tested conventional solvents. In addition, we optimised by response surface methodology the integrated process (2.0% m/m, 25.0 °C, pH 9.0), which was equally effective (ca. 2900 µg PEITC/g watercress), regardless of the surfactant used. The optimal extracts showed greater antioxidant capacity than pure PEITC, due to other antioxidant compounds extracted in the process. In conclusion, by the present work, we developed an innovative cost-effective and low environmental impact process for obtaining PEITC extracts from watercress by-products.
Efficient Malathion Removal in Constructed Wetlands Coupled to UV/H2O2 Pretreatment
Authors: Cedillo-Herrera Cinthia I. G. , Roé-Sosa Adriana , Pat-Espadas Aurora M. , Ramírez Karina , Rochín-Medina Jaime , Amabilis-Sosa Leonel E.
Published: August 2020

Intensive agriculture has led to the increasing application of pesticides, such as malathion, thus generating large volumes of untreated cropland wastewater (CropWW). In this work, a hybrid system constructed wetlands (CW) coupled in continuous with an optimized UV/H2O2 pretreatment was evaluated for the efficient removal of malathion contained in CropWW. In the first stage, 90 min UV irradiation time (UV IR) and 65 mM hydrogen peroxide (H2O2) were identified as optimal operation parameters through a central composite design. The second stage consisted of CW planted with Phragmites australis collected from the agricultural discharge area and operated as a piston flow reactor. Furthermore, CW hydraulic residence times (HRT) of 1, 2 and 3 days, including hydraulic coupling, were evaluated. The removal efficiencies obtained in the first stage (UV/H2O2) were 94 ± 2.5% of malathion and 45 ± 2.5% of total organic carbon (TOC). In stage two (CW) 65 ± 9.6% TOC removal was achieved during the first 17 days, from which around 24% was associated to the biosorption of malathion byproducts. Subsequently, and until the operation ends, CW removed about 80% of TOC for 2 and 3 days HRT, with no significant differences (p > 0.2), which is higher than those reported in several studies involving only advanced oxidation processes (AOP) with UV IR times above 240 min and even for systems using catalysts. The results obtained indicate that the system UV/H2O2-CW is a technically suitable option for the treatment of CropWW with a high content of malathion mainly found in developing countries. Moreover, the hybrid system proposed also represent significant reduction in the size of the treatment plant.
Theoretical Design of a Novel Vibration Energy Absorbing Mechanism for Cables
Authors: Qin Zhen , Wu Yu-Ting , Huang Aihua , Lyu Sung-Ki , Sutherland John W.
Published: August 2020

A novel design of a vibration energy absorbing mechanism (VEAM) that is based on multi-physics (magnetic spring, hydraulic system, structural dynamics, etc.) for cable vibration is proposed. The minimum working force of the hydraulic cylinder has been exploited in this design in order to combine a non-linear stiffness vibration isolation module that is composed of permanent magnetic springs with hydraulic viscous vibration damping modules. In response to different environmental vibration impacts, VEAM can automatically switch the vibration control modes without an electronic mechanism. Additionally, the non-contact design effectively reduces the wear that is induced by the reciprocating motion of the small amplitude of the hydraulic viscous dampers. The proposed mechanism is explained and a theoretical model is established. The transmissibility of the two modules at a single degree of freedom is derived using the harmonic balance method. After that, a series of variable control numerical simulations were performed for each important parameter. Empirical rules for designing the system were created by comparing the influence of each parameter on the vibration isolation performance of the entire system.
Off-Design Operation of Conventional and Phase-Change CO2 Capture Solvents and Mixtures: A Systematic Assessment Approach
Authors: Zarogiannis Theodoros , Papadopoulos Athanasios I. , Seferlis Panos
Published: August 2020

Solvent-based CO2 capture technologies hold promise for future implementation but conventional solvents incur significant energy penalties and capture costs. Phase-change solvents enable a significant reduction in the regeneration energy but their performance has only been investigated under steady-state operation. In the current work, we employed a systematic approach for the evaluation of conventional solvents and mixtures, as well as phase-change solvents under the influence of disturbances. Sensitivity analysis was used to identify the impact that operating parameter variations and different solvents exert on multiple CO2 capture performance indicators within a wide operating range. The resulting capture process performance was then assessed for each solvent within a multi-criteria approach, which simultaneously accounted for off-design conditions and nominal operation. The considered performance criteria included the regeneration energy, solvent mass flow rate, cost and cyclic capacity, net energy penalty from integration with an upstream power plant, and lost revenue from parasitic losses. The 10 investigated solvents included the phase-change solvents methyl-cyclohexylamine (MCA) and 2-(diethylamino)ethanol/3-(methylamino)propylamine (DEEA/MAPA). We found that the conventional mixture diethanolamine/methyldiethanolamine (DEA/MDEA) and the phase-change solvent DEEA/MAPA exhibited both resilience to disturbances and desirable nominal operation for multiple performance indicators simultaneously.
Contribution to the Physical Modelling of Single Charged Defects Causing the Random Telegraph Noise in Junctionless FinFET
Authors: Atamuratov Atabek E. , Khalilloev Mahkam M. , Yusupov Ahmed , García-Loureiro A. J. , Chedjou Jean Chamberlain , Kyandoghere Kyamakya
Published: August 2020

In this paper, different physical models of single trap defects are considered, which are localized in the oxide layer or at the oxide–semiconductor interface of field effect transistors. The influence of these defects with different sizes and shapes on the amplitude of the random telegraph noise (RTN) in Junctionless Fin Field Effect Transistor (FinFET) is modelled and simulated. The RTN amplitude dependence on the number of single charges trapped in a single defect is modelled and simulated too. It is found out that the RTN amplitude in the Junctionless FinFET does not depend on the shape, nor on the size of the single defect area. However, the RTN amplitude in the subthreshold region does considerably depend on the number of single charges trapped in the defect.
Inferring Emotion Tags from Object Images Using Convolutional Neural Network
Authors: Manzoor Anam , Ahmad Waqar , Ehatisham-ul-Haq Muhammad , Hannan Abdul , Khan Muhammad Asif , Ashraf M. Usman , Alghamdi Ahmed M. , Alfakeeh Ahmed S.
Published: August 2020

Emotions are a fundamental part of human behavior and can be stimulated in numerous ways. In real-life, we come across different types of objects such as cake, crab, television, trees, etc., in our routine life, which may excite certain emotions. Likewise, object images that we see and share on different platforms are also capable of expressing or inducing human emotions. Inferring emotion tags from these object images has great significance as it can play a vital role in recommendation systems, image retrieval, human behavior analysis and, advertisement applications. The existing schemes for emotion tag perception are based on the visual features, like color and texture of an image, which are poorly affected by lightning conditions. The main objective of our proposed study is to address this problem by introducing a novel idea of inferring emotion tags from the images based on object-related features. In this aspect, we first created an emotion-tagged dataset from the publicly available object detection dataset (i.e., “Caltech-256”) using subject evaluation from 212 users. Next, we used a convolutional neural network-based model to automatically extract the high-level features from object images for recognizing nine (09) emotion categories, such as amusement, awe, anger, boredom, contentment, disgust, excitement, fear, and sadness. Experimental results on our emotion-tagged dataset endorse the success of our proposed idea in terms of accuracy, precision, recall, specificity, and F1-score. Overall, the proposed scheme achieved an accuracy rate of approximately 85% and 79% using top-level and bottom-level emotion tagging, respectively. We also performed a gender-based analysis for inferring emotion tags and observed that male and female subjects have discernment in emotions perception concerning different object categories.
Designing Multi-Agent System Organisations for Flexible Runtime Behaviour
Authors: Keogh Kathleen , Sonenberg Liz
Published: August 2020

We address the challenge of multi-agent system (MAS) design for organisations of agents acting in dynamic and uncertain environments where runtime flexibility is required to enable improvisation through sharing knowledge and adapting behaviour. We identify behavioural features that correspond to runtime improvisation by agents in a MAS organisation and from this analysis describe the OJAzzIC meta-model and an associated design method. We present results from simulation scenarios, varying both problem complexity and the level of organisational support provided in the design, to show that increasing design time guidance in the organisation specification can enable runtime flexibility afforded to agents and improve performance. Hence the results demonstrate the usefulness of the constructs captured in the OJAzzIC meta-model.
Carotenoids, Polyphenols, and Ascorbic Acid in Organic Rosehips (Rosa spp.) Cultivated in Lithuania
Authors: Medveckienė Brigita , Kulaitienė Jurgita , Jarienė Elvyra , Vaitkevičienė Nijolė , Hallman Ewelina
Published: August 2020

Carotenoids, polyphenols, and ascorbic acid are valuable and important biologically active compounds that contribute to the health benefits of different foods, and rosehips are known for their high biologically active substance contents. The aim of this research is to identify and compare the contents of carotenoids, polyphenols, and ascorbic acid in the flesh and seeds of different rosehip species grown organically. A field experiment was conducted during the period 2017–2018 on an organic farm in Lithuania. Rose fruits were collected at full ripening in September. The quantitative and qualitative determinations of polyphenols and carotenoids in rosehips flesh and seeds were conducted by HPLC methods. The following polyphenolic compounds were identified: five different flavonoids (rutin, kaempferol-3-O-glucoside, luteolin, quercetin, and quercetin-3-O-glucoside) and five phenolic acids (gallic, chlorogenic, caffeic, p-coumaric, and ferulic) from different rose fruit fleshes and seeds species. In addition, six carotenoids (β-carotene, α-carotene, lutein, zeaxanthin, cis-lycopene, and trans-lycopene) in rosehip flesh and five different carotenoids in rosehip seeds (α-carotene, lutein, zeaxanthin, cis-lycopene, and trans-lycopene) were identified. Overall, the results of this study demonstrate that the highest content of total phenolic acid is in rosehip seeds, while higher concentrations of carotenoids, flavonoids, and ascorbic acid are present in rosehip flesh.
Vesicular Emulgel Based System for Transdermal Delivery of Insulin: Factorial Design and in Vivo Evaluation
Authors: Shehata Tamer M. , Nair Anroop B. , Al-Dhubiab Bandar E. , Shah Jigar , Jacob Shery , Alhaider Ibrahim A. , Attimarad Mahesh , Elsewedy Heba S. , Ibrahim Mahmoud M.
Published: August 2020

Transdermal delivery of insulin is a great challenge due to its poor permeability through the skin. The aim of the current investigation was to evaluate the prospective of insulin loaded niosome emulgel as a noninvasive delivery system for its transdermal therapy. A 23 full-factorial design was used to optimize the insulin niosome emulgel by assessing the effect of independent variables (concentration of paraffin oil, Tween 80 and sodium carboxymethyl cellulose) on dependent variables (in vitro release, viscosity and in vitro permeation). The physical characteristics of the prepared formulations were carried out by determining viscosity, particle size, entrapment efficiency, drug loading, drug release and kinetics. In vitro permeation studies were carried out using rat skin membrane. Hypoglycemic activity of prepared formulations was assessed in diabetic-induced rats. It was observed that the independent variables influenced the dependent variables. A significant difference (p < 0.05) in viscosity was noticed between the prepared gels, which in turn influenced the insulin release. The order of permeation is: insulin niosome emulgel > insulin niosome gel > insulin emulgel > insulin gel > insulin niosomes > insulin solution. The enhancement in transdermal flux in insulin niosome emulgel was 10-fold higher than the control (insulin solution). In vivo data significantly demonstrated reduction (p < 0.05) of plasma glucose level (at six hours) by insulin niosome emulgel than other formulations tested. The results suggest that the developed insulin niosome emulgel could be an efficient carrier for the transdermal delivery of insulin.
Epoxy/Glass Fiber Nanostructured p- and n-Type Thermoelectric Enabled Model Composite Interphases
Authors: Karalis George , Tsirka Kyriaki , Tzounis Lazaros , Mytafides Christos , Koutsotolis Lampros , Paipetis Alkiviadis S.
Published: August 2020

This experimental study is associated with the modification of glass fibers with efficient, organic, functional, thermoelectrically enabled coatings. The thermoelectric (TE) behavior of the coated glass fiber tows with either inherent p semiconductor type single wall carbon nanotubes (SWCNTs) or the n-type molecular doped SWCNTs were examined within epoxy resin matrix in detail. The corresponding morphological, thermogravimetric, spectroscopic, and thermoelectric measurements were assessed in order to characterize the produced functional interphases. For the p-type model composites, the Seebeck coefficient was +16.2 μV/K which corresponds to a power factor of 0.02 μW/m∙K2 and for the n-type −28.4 μV/K which corresponds to power factor of 0.12 μW/m∙K2. The p–n junction between the model composites allowed for the fabrication of a single pair thermoelectric element generator (TEG) demonstrator. Furthermore, the stress transfer at the interphase of the coated glass fibers was studied by tow pull-out tests. The reference glass fiber tows presented the highest interfacial shear stress (IFSS) of 42.8 MPa in comparison to the p- and n-type SWCNT coated GF model composites that exhibited reduced IFSS values by 10.1% and 28.1%, respectively.