bluuebunny/crossref_metadata_2025 huggingface dataset:
Feasibility of a 12 Week Physical Intervention to Prevent Cognitive Decline and Disability in the At-Risk Elderly Population in Korea
Authors: Lee Sun Min , Song Hong-sun , Chun Buong-O , Choi Muncheong , Sun Kyunghwa , Kim Ki Sub , Jeon Hyesu , Seo Da Eun , Kwon Hye Mi , Jeong Jee Hyang , Park Yoo Kyoung , Hong Chang Hyung , Na Hae Ri , Choi Seong Hye , Moon So Young
Published: September 2020

There is a need for measures that can prevent the onset of dementia in the rapidly aging population. Reportedly, sustained physical exercise can prevent cognitive decline and disability. This study aimed to assess the feasibility of a 12-week physical exercise intervention (PEI) for delay of cognitive decline and disability in the at-risk elderly population in Korea. Twenty-six participants (aged 67.9 ± 3.6 years, 84.6% female) at risk of dementia were assigned to facility-based PEI (n = 15) or home-based PEI (n = 11). The PEI program consisted of muscle strength training, aerobic exercise, balance, and stretching using portable aids. Feasibility was assessed by retention and adherence rates. Physical fitness/cognitive function were compared before and after the PEI. Retention and adherence rates were 86.7% and 88.3%, respectively, for facility-based PEI and 81.8% and 62.3% for home-based PEI. No intervention-related adverse events were reported. Leg strength/endurance and cardiopulmonary endurance were improved in both groups: 30 s sit-to-stand test (facility-based, p = 0.002; home-based, p = 0.002) and 2 -min stationary march (facility-based, p = 0.001; home-based, p = 0.022). Cognitive function was improved only after facility-based PEI (Alzheimer’s Disease Assessment Scale-cognitive total score, p = 0.009; story memory test on Literacy Independent Cognitive Assessment, p = 0.026). We found that, whereas our PEI is feasible, the home-based program needs supplementation to improve adherence.
Stroke as a Potential Complication of COVID-19-Associated Coagulopathy: A Narrative and Systematic Review of the Literature
Authors: Szegedi István , Orbán-Kálmándi Rita , Csiba László , Bagoly Zsuzsa
Published: September 2020

Coronavirus disease 2019 (COVID-19) is the most overwhelming medical threat of the past few decades. The infection, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), can cause serious illness leading to respiratory insufficiency, and, in severely ill patients, it can progress to multiple organ failure leading to death. It has been noted from the earliest reports that the disease influences the hemostasis system and a hallmark of severe infection is elevated D-dimer levels. The profound coagulation changes in COVID-19 seem to be linked to inflammation-related events and severe endothelial cell injury. Besides the high incidence of venous thromboembolic events in SARS-CoV-2 infections, arterial events, including cerebrovascular events, were found to be associated with the disease. In this review, we aimed to summarize the available literature on COVID-19-associated coagulopathy and thrombosis. Furthermore, we performed a systematic search of the literature to identify the characteristics of stroke in COVID-19. Our findings showed that acute ischemic stroke (AIS) is the most frequent type of stroke occurring in infected patients. In most cases, stroke was severe (median NIHSS:16) and most of the patients had one or more vascular risk factors. Laboratory findings in AIS patients were consistent with COVID-19-associated coagulopathy, and elevated D-dimer levels were the most common finding. The outcome was unfavorable in most cases, as a large proportion of the reported patients died or remained bedridden. Limited data are available as yet on outcomes after acute vascular interventions in COVID-19 patients. In the future, well-designed studies are needed to better understand the risk of stroke in COVID-19, to optimize treatment, and to improve stroke care.
The HLA-Cw6 Dilemma: Is It Really an Outcome Predictor in Psoriasis Patients under Biologic Therapy? A Monocentric Retrospective Analysis
Authors: Burlando Martina , Russo Roberto , Clapasson Andrea , Carmisciano Luca , Stecca Anna , Cozzani Emanuele , Parodi Aurora
Published: September 2020

HLA-Cw6 is one of the most strongly associated psoriasis susceptibility alleles. Data regarding correlation between HLA-Cw6 status and biologic treatment outcomes are divergent. The aim of our study in our cohort of psoriatic patients was to explore if the HLA-Cw6 status influences the response rate to biologic therapies at 16 and 48 weeks. One hundred and one psoriatic patients eligible for biologic therapies were enrolled. HLA-C*06 alleles were detected from their blood samples. The effectiveness of antipsoriatic treatments was reported as 90% Psoriasis Area and Severity Index reduction (PASI90). All biologics showed efficacy at week 16, without significant differences between one another. HLA-Cw6 status did not seem to affect baseline characteristics, or treatment response at week 16. At week 48, IL-12/23 and IL-17 targeting drugs were more effective on Cw6-positive patients than on Cw6-negative patients. Conversely, TNF-targeting drugs seemed to be more effective on Cw6- negative patients than on Cw6-positive patients. The HLA-Cw6 test could well deserve to be integrated into the clinical laboratory work-up supporting the choice of the correct biologic.
Personalized Medicine for Antibiotics: The Role of Nanobiosensors in Therapeutic Drug Monitoring
Authors: Garzón Vivian , Bustos Rosa-Helena , G. Pinacho Daniel
Published: September 2020

Due to the high bacterial resistance to antibiotics (AB), it has become necessary to adjust the dose aimed at personalized medicine by means of therapeutic drug monitoring (TDM). TDM is a fundamental tool for measuring the concentration of drugs that have a limited or highly toxic dose in different body fluids, such as blood, plasma, serum, and urine, among others. Using different techniques that allow for the pharmacokinetic (PK) and pharmacodynamic (PD) analysis of the drug, TDM can reduce the risks inherent in treatment. Among these techniques, nanotechnology focused on biosensors, which are relevant due to their versatility, sensitivity, specificity, and low cost. They provide results in real time, using an element for biological recognition coupled to a signal transducer. This review describes recent advances in the quantification of AB using biosensors with a focus on TDM as a fundamental aspect of personalized medicine.
Characterisation of MRI Indeterminate Breast Lesions Using Dedicated Breast PET and Prone FDG PET-CT in Patients with Breast Cancer—A Proof-of-Concept Study
Authors: Malhotra Anmol , Tincey Sophia , Naidu Vishnu , Papagiorcopulo Carla , Ghosh Debashis , Tan Peng H. , Wickham Fred , Wagner Thomas
Published: September 2020

Magnetic resonance imaging (MRI) in patients with breast cancer to assess extent of disease or multifocal disease can demonstrate indeterminate lesions requiring second-look ultrasound and ultrasound or MRI-guided biopsies. Prone positron emission tomography-computed tomography (PET-CT) is a dedicated acquisition performed with a breast-supporting device on a standard PET-CT scanner. The MAMmography with Molecular Imaging (MAMMI, Oncovision, Valencia, Spain) PET system (PET-MAMMI) is a true tomographic ring scanner for the breast. We investigated if PET-MAMMI and prone PET-CT were able to characterise these MRI- indeterminate lesions further. A total of 10 patients with breast cancer and indeterminate lesions on breast MRI were included. Patients underwent prone PET-MAMMI and prone PET-CT after injection of FDG subsequently on the same day. Patients then resumed their normal pathway, with the clinicians blinded to the results of the PET-MAMMI and prone PET-CT. Of the MRI-indeterminate lesions, eight were histopathologically proven to be malignant and two were benign. PET-MAMMI and prone PET-CT only were able to demonstrate increased FDG uptake in 1/8 and 0/8 of the MRI-indeterminate malignant lesions, respectively. Of the MRI-indeterminate benign lesions, both PET-MAMMI and prone PET-CT demonstrated avidity in 1/2 of these lesions. Our findings do not support the use of PET-MAMMI to characterise indeterminate breast MRI lesions requiring a second look ultrasound.
Clinical and Laboratory Associations with Methotrexate Metabolism Gene Polymorphisms in Rheumatoid Arthritis
Authors: D’Cruz Leon G. , McEleney Kevin G. , Tan Kyle B. C. , Shukla Priyank , Gardiner Philip V. , Connolly Patricia , Conway Caroline , Cobice Diego , Gibson David S.
Published: September 2020

Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that causes loss of joint function and significantly reduces quality of life. Plasma metabolite concentrations of disease-modifying anti-rheumatic drugs (DMARDs) can influence treatment efficacy and toxicity. This study explored the relationship between DMARD-metabolising gene variants and plasma metabolite levels in RA patients. DMARD metabolite concentrations were determined by tandem mass-spectrometry in plasma samples from 100 RA patients with actively flaring disease collected at two intervals. Taqman probes were used to discriminate single-nucleotide polymorphism (SNP) genotypes in cohort genomic DNA: rs246240 (ABCC1), rs1476413 (MTHFR), rs2231142 (ABCG2), rs3740065 (ABCC2), rs4149081 (SLCO1B1), rs4846051 (MTHFR), rs10280623 (ABCB1), rs16853826 (ATIC), rs17421511 (MTHFR) and rs717620 (ABCC2). Mean plasma concentrations of methotrexate (MTX) and MTX-7-OH metabolites were higher (p < 0.05) at baseline in rs4149081 GA genotype patients. Patients with rs1476413 SNP TT or CT alleles have significantly higher (p < 0.001) plasma poly-glutamate metabolites at both study time points and correspondingly elevated disease activity scores. Patients with the rs17421511 SNP AA allele reported significantly lower pain scores (p < 0.05) at both study intervals. Genotyping strategies could help prioritise treatments to RA patients most likely to gain clinical benefit whilst minimizing toxicity.
Quantifying Climate-Wise Connectivity across a Topographically Diverse Landscape
Authors: Gray Morgan , Micheli Elisabeth , Comendant Tosha , Merenlender Adina
Published: September 2020

Climate-wise connectivity is essential to provide species access to suitable habitats in the future, yet we lack a consistent means of quantifying climate adaptation benefits of habitat linkages. Species range shifts to cooler climates have been widely observed, suggesting we should protect pathways providing access to cooler locations. However, in topographically diverse regions, the effects of elevation, seasonality, and proximity to large water bodies are complex drivers of biologically relevant temperature gradients. Here, we identify potential terrestrial and riparian linkages and their cooling benefit using mid-century summer and winter temperature extremes for interior coastal ranges in Northern California. It is rare for the same area to possess both terrestrial and riparian connectivity value. Our analysis reveals distinct differences in the magnitude and orientation of cooling benefits between the summer maximum and winter minimum temperatures provided by the linkages we delineated for the area. The cooling benefits for both linkage types were maximized to the west during summer, but upslope and to the northeast during winter. The approach we employ here provides an improved method to prioritize climate-wise connectivity and promote landscape resilience for topographically diverse regions.
Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties
Authors: Adnan Md. , Oh Ki Kwang , Azad Md Obyedul Kalam , Shin Myung Hwan , Wang Myeong-Hyeon , Cho Dong Ha
Published: September 2020

Hibiscus cannabinus (Kenaf) is a potential source of bioactive constituents and natural antioxidant. The current study determined the impact of various solvents on extraction yield, recovery of polyphenol and flavonoid, antioxidant, anticancer, and antibacterial properties of Kenaf leaves and seed. The powder of leaves and seed was separately extracted with n-hexane, ethyl acetate, ethanol, and water solvent. Among them, the ethanol extract of leaves and seed showed the highest extraction yield, and their GC-MS analysis revealed a total of 55 and 14 bioactive compounds, respectively. The total polyphenols (TP) and flavonoids (TF) content were quantified by a spectrophotometric technique where water extracts displayed a noteworthy amount of TP and TF content compared to other extracts. A similar demonstration was noticed in antioxidant activity, evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydrogen peroxide scavenging capacity. In addition, cytotoxicity and anti-lung cancer activity were identified against mouse embryonic fibroblast (NIH3T3) and human lung cancer (A549) cells. All extracts of leaves and seed were observed as non-toxic to the NIH3T3 cells, but slight toxicity was expressed by n-hexane extracts at the optimum dose (1000 µg/mL) of treatment. In parallel, n-hexane and ethanol extracts (leaves and seed) exposed promising anti-lung cancer activity at the same concentration. Furthermore, antibacterial activity was assessed using disc diffusion assay, and seed extracts exhibited a significant inhibition zone against Gram-positive and Gram-negative microorganisms. Overall, Kenaf seed extracted with polar solvents was found very potent in terms of important bioactive compounds and pharmacological aspects, which can be an excellent biological matrix of natural antioxidants.
Polyurethane-Based Coatings with Promising Antibacterial Properties
Authors: Villani Maurizio , Bertoglio Federico , Restivo Elisa , Bruni Giovanna , Iervese Stefano , Arciola Carla Renata , Carulli Francesco , Iannace Salvatore , Bertini Fabio , Visai Livia
Published: September 2020

In coatings technology, the possibility of introducing specific characteristics at the surface level allows for the manufacture of medical devices with efficient and prolonged antibacterial properties. This efficiency is often achieved by the use of a small amount of antibacterial molecules, which can fulfil their duty while limiting eventual releasing problems. The object of this work was the preparation and characterization of silver, titanium dioxide and chitosan polyurethane-based coatings. Coatings with the three antibacterials were prepared using different deposition techniques, using a brush or a bar coater automatic film applicator, and compared to solvent casted films prepared with the same components. For silver containing materials, an innovative strategy contemplating the use and preparation of silver nanoparticles in a single step-method was employed. This preparation was obtained starting from a silver precursor and using a single compound as the reducing agent and stabilizer. Ultraviolet-visible spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, contact angle measurements and adhesion test experiments were used to characterize the prepared coatings. Promising antibacterial properties, measured via direct and indirect methods, were registered for all the silver-based materials.
Calcium Phosphate Coating Prepared by Microarc Oxidation Affects hTERT Expression, Molecular Presentation, and Cytokine Secretion in Tumor-Derived Jurkat T Cells
Authors: Litvinova Larisa S. , Khaziakhmatova Olga G. , Shupletsova Valeria V. , Yurova Kristina A. , Malashchenko Vladimir V. , Shunkin Egor O. , Ivanov Pavel A. , Komarova Ekaterina G. , Chebodaeva Valentina V. , Porokhova Ekaterina D. , Gereng Elena A. , Khlusov Igor A.
Published: September 2020

Calcium phosphate (CaP) materials are among the best bone graft substitutes, but their use in the repair of damaged bone in tumor patients is still unclear. The human Jurkat T lymphoblast leukemia-derived cell line (Jurkat T cells) was exposed in vitro to a titanium (Ti) substrate (10 × 10 × 1 mm3) with a bilateral rough (average roughness index (Ra) = 2–5 μm) CaP coating applied via the microarc oxidation (MAO) technique, and the morphofunctional response of the cells was studied. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectroscope (EDX) analyses showed voltage-dependent (150–300 V) growth of structural (Ra index, mass, and thickness) and morphological surface and volume elements, a low Ca/PaT ratio (0.3–0.6), and the appearance of crystalline phases of CaHPO4 (monetite) and β-Ca2P2O7 (calcium pyrophosphate). Cell and molecular reactions in 2-day and 14-day cultures differed strongly and correlated with the Ra values. There was significant upregulation of hTERT expression (1.7-fold), IL-17 secretion, the presentation of the activation antigens CD25 (by 2.7%) and CD95 (by 5.15%) on CD4+ cells, and 1.5–2-fold increased cell apoptosis and necrosis after two days of culture. Hyperactivation-dependent death of CD4+ cells triggered by the surface roughness of the CaP coating was proposed. Conversely, a 3.2-fold downregulation in hTERT expression increased the percentages of CD4+ cells and their CD95+ subset (by 15.5% and 22.9%, respectively) and inhibited the secretion of 17 of 27 test cytokines/chemokines without a reduction in Jurkat T cell survival after 14 days of coculture. Thereafter, cell hypoergy and the selection of an hTERT-independent viable CD4+ subset of tumor cells were proposed. The possible role of negative zeta potentials and Ca2+ as effectors of CaP roughness was discussed. The continuous (2–14 days) 1.5–6-fold reductions in the secretion of vascular endothelial growth factor (VEGF) by tumor cells correlated with the Ra values of microarc CaP-coated Ti substrates seems to limit surgical stress-induced metastasis of lymphoid malignancies.
Primary Stability of Revision Acetabular Reconstructions Using an Innovative Bone Graft Substitute: A Comparative Biomechanical Study on Cadaveric Pelvises
Authors: Morosato Federico , Traina Francesco , Schierjott Ronja A. , Hettich Georg , Grupp Thomas M. , Cristofolini Luca
Published: September 2020

Hip implant failure is mainly due to aseptic loosening of the cotyle and is typically accompanied by defects in the acetabular region. Revision surgery aims to repair such defects before implantation by means of reconstruction materials, whose morselized bone graft represents the gold standard. Due to the limited availability of bone tissue, synthetic substitutes are also used. The aim of this study was to evaluate if a synthetic fully resorbable tri-calcium phosphate-based substitute can provide adequate mechanical stability when employed to restore severe, contained defects, in comparison with morselized bone graft. Five cadaveric pelvises were adopted, one side was reconstructed with morselized bone graft and the other with the synthetic substitute, consisting of dense calcium phosphate granules within a collagen matrix. During the biomechanical test, cyclic load packages of increasing magnitude were applied to each specimen until failure. Bone/implant motions were measured through Digital Image Correlation and were expressed in terms of permanent and inducible translations and rotations. The reconstruction types exhibited a similar behavior, consisting of an initial settling trend followed by failure as bone fracture (i.e., no failure of the reconstruction material). When 2.2 Body Weight was applied, the permanent translations were not significantly different between the two reconstructions (p = 0.06–1.0) and were below 1.0 mm. Similarly, the inducible translations did not differ significantly (p = 0.06–1.0) and were below 0.160 mm. Rotations presented the same order of magnitude but were qualitatively different. Overall, the synthetic substitute provided adequate mechanical stability in comparison with morselized bone graft, thus representing a reliable alternative to treat severe, contained acetabular defects.
Influence of Laser-Welding on Microstructure and Corrosion Properties of Twinning-Induced Plasticity (TWIP) Steel
Authors: Xu Chengcheng , Zhang Youkang , Liu Wanlei , Jin Ying , Wen Lei , Sun Dongbai
Published: September 2020

The effect of welding speed on microstructure, mechanical properties, and corrosion properties of laser-assisted welded joints of a twinning-induced plasticity (TWIP) steel was investigated by using X-ray diffraction (XRD), scanning electron microscopy (SEM), electron backscattered diffraction (EBSD) analysis, electrochemical test, and micro-area scanning Kelvin probe test (SKP). The results reveal that the welded joints, with a fully austenitic structure, are obtained by laser welding. In addition, the preferred orientation of grains in fusion zone (FZ) increased with the increase of welding speed. Additionally, the coincidence site lattice (CSL) grain boundaries of FZ decreased with increasing welding speed. However, potentiodynamic polarization and SKP results demonstrated that the welding speed of 1.5 m/min renders superior corrosion resistance. It can also be inferred that the corrosion properties of the welded joints are related to the grain size and frequency of CSL grain boundary in FZ.
Effects of Bonding Agents on Metal-Ceramic Bond Strength of Co-Cr Alloys Fabricated by Selective Laser Melting
Authors: Yoo Soo-Yoen , Kim Seong-Kyun , Heo Seong-Joo , Koak Jai-Young , Kim Joung-Gyu
Published: September 2020

Bonding agents have been developed to improve bond strength between ceramic and Co-Cr metal. The aim of this study was to investigate the influence of two bonding agents on bond strength of Co-Cr metal fabricated by selective laser melting (SLM). Bond strength was determined by a three-point bending test, and the interfaces of the metal and ceramic, before and after the bending test, were observed by optical microscopy and scanning electron microscopy (SEM) to determine the thickness of the oxide layer and amount of ceramic remaining. To analyze the elemental composition of the bonding agents and fractured surfaces, energy dispersive X-ray spectroscopy (EDS) was used. Co-Cr specimens with bonding agent showed significantly higher bond strength than Co-Cr specimens without bonding agents. The fractured surfaces of most specimens showed mixed failure, but failure mode varied according to bonding agent and fabrication type. Specimens from groups treated with bonding agents had significantly higher remaining ceramic fractions on fractured Co-Cr alloys than specimens from groups that did not receive bonding agent. Mass amounts of silicone (Si) and titanium (Ti) on the fractured alloy surfaces were also different among specimens according to method of fabrication and presence of bonding agent. Together, the results suggest that application of bonding agent to 3D printed Co-Cr metal increases bond strength with ceramics.
Slicing Ceramics on Material Removed by a Single Abrasive Particle
Authors: Tsai Yao-Yang , Wu Ming-Chang , Liao Yunn-Shiuan , Tsao Chung-Chen , Hsu Chun-Yao
Published: September 2020

Multi-wire saw machining (MWSM) used for slicing hard-brittle materials in the semiconductor and photovoltaic industries is an important and efficient material removal process that uses free abrasives. The cutting model of single-wire saw machining (SWSM) is the basis of MWSM. The material removal mechanism of SWSM is more easily understood than MWSM. A mathematical model (includes brittle fracture and plastic deformation) is presented in this paper for SWSM ceramic with abrasives. This paper determines the effect of various machining parameters on the removal of hard-brittle materials. For brittle fracture of SWSM ceramics, the minimum strain energy density is used as a fracture criterion. For plastic deformation of SWSM ceramics, the material removal is calculated using equations of motion. Actual wire-sawing experiments are conducted to verify the results of the developed mathematical model. The theoretical results agree with experimental data and practical experience. From the developed mathematical model, brittle fracture plays a major role in material removal of SWSM ceramics. Wire speed (S) and working load (P) are positively correlated with material removal of SWSM ceramics. The coefficient of friction is low, a lateral crack, which propagates almost parallel to the working surface, leads to more brittle fracture and material removal is increased.
Importance of Susceptibility Rate of ‘the First’ Isolate: Evidence of Real-World Data
Authors: Kim Sollip , Yoo Soo Jin , Chang Jeonghyun
Published: September 2020

Background and objectives: For proper antimicrobial therapy, cumulative antibiograms should be representative of geographic region and be accurate. Clinical and Laboratory Standards Institute (CLSI) guidelines recommend that only the first isolates (FI) of a species per patient are used when reporting cumulative antibiograms. However, >50% of hospitals in the United States report antibiograms of all isolates. We compared antibiograms from the FI with those from total isolates (TI). Materials and Methods: Antimicrobial data of all isolates identified in the Microbiology unit of Ilsan Paik Hospital in 2019 were retrospectively acquired from the hospital information system. The susceptibility rates to antimicrobials of Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecium, and Enterococcus faecalis were analyzed by FI and TI, respectively. Isolate counts and susceptibility rates of each species for the reported antimicrobials were compared. Results: The numbers of isolates by FI/TI were as follows: 1824/2692 E. coli, 480/1611 A. baumannii, and 662/1306 K. pneumoniae, and 407/953 P. aeruginosa for gram-negative bacteria and 649/1364 S. aureus, 211/313 E. faecium, and 323/394 E. faecalis for gram-positive bacteria. All antimicrobial agents showed higher susceptibility rates when calculated as FI than as TI in gram-negative bacteria except colistin: 3.7% for E. coli, 14.5% for A. baumannii, 8.3% for K. pneumoniae, and 7.9% for P. aeruginosa. In S. aureus, 8/11 antimicrobial agents revealed higher susceptibility rates for FI than for TI. E. faecalis and E. faecium showed lower susceptibility rates for 7/10 antimicrobial agents for FI than for TI. The oxacillin susceptibility rates of S. aureus were 36.6%/30.2% with FI/TI and vancomycin susceptibility rates for E. faecium were 54.1% and 49.5%, respectively. Conclusions: When comparing cumulative antibiograms by FI with TI using real-world data, there is a large gap for critical species requiring hospital infection control. Although FI calculation is difficult, antibiograms must be calculated as FI for proper preemptive antimicrobial therapy because FI provides proper antimicrobial susceptibility data.
Modification of Polysulfone Ultrafiltration Membranes via Addition of Anionic Polyelectrolyte Based on Acrylamide and Sodium Acrylate to the Coagulation Bath to Improve Antifouling Performance in Water Treatment
Authors: Plisko Tatiana V. , Bildyukevich Alexandr V. , Burts Katsiaryna S. , Hliavitskaya Tatiana A. , Penkova Anastasia V. , Ermakov Sergey S. , Ulbricht Mathias
Published: September 2020

Surface modification of polysulfone ultrafiltration membranes was performed via addition of an anionic polymer flocculant based on acrylamide and sodium acrylate (PASA) to the coagulation bath upon membrane preparation by non-solvent induced phase separation (NIPS). The effect of PASA concentration in the coagulant at different coagulation bath temperatures on membrane formation time, membrane structure, surface roughness, hydrophilic-hydrophobic balance of the skin layer, surface charge, as well as separation and antifouling performance was studied. Scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, contact angle and zeta potential measurements were utilized for membrane characterization. Membrane barrier and antifouling properties were evaluated in ultrafiltration of model solutions containing human serum albumin and humic acids as well as with real surface water. PASA addition was found to affect the kinetics of phase separation leading to delayed demixing mechanism of phase separation due to the substantial increase of coagulant viscosity, which is proved by a large increase of membrane formation time. Denser and thicker skin layer is formed and formation of macrovoids in membrane matrix is suppressed. FTIR analysis confirms the immobilization of PASA macromolecules into the membrane skin layer, which yields improvement of hydrophilicity and change of zeta potential. Modified membrane demonstrated better separation and antifouling performance in the ultrafiltration of humic acid solution and surface water compared to the reference membrane.
Unintended Changes of Ion-Selective Membranes Composition—Origin and Effect on Analytical Performance
Authors: Maksymiuk Krzysztof , Stelmach Emilia , Michalska Agata
Published: September 2020

Ion-selective membranes, as used in potentiometric sensors, are mixtures of a few important constituents in a carefully balanced proportion. The changes of composition of the ion-selective membrane, both qualitative and quantitative, affect the analytical performance of sensors. Different constructions and materials applied to improve sensors result in specific conditions of membrane formation, in consequence, potentially can result in uncontrolled modification of the membrane composition. Clearly, these effects need to be considered, especially if preparation of miniaturized, potentially disposable internal-solution free sensors is considered. Furthermore, membrane composition changes can occur during the normal operation of sensors—accumulation of species as well as release need to be taken into account, regardless of the construction of sensors used. Issues related to spontaneous changes of membrane composition that can occur during sensor construction, pre-treatment and their operation, seem to be underestimated in the subject literature. The aim of this work is to summarize available data related to potentiometric sensors and highlight the effects that can potentially be important also for other sensors using ion-selective membranes, e.g., optodes or voltammetric sensors.
Heat Input Influence on the Fatigue Life of Welds from Steel S460MC
Authors: Moravec Jaromir , Sobotka Jiri , Solfronk Pavel , Thakral Robin
Published: September 2020

Fine-grained steels belong to the progressive materials, which are increasingly used in the production of welded structures subjected to both static and dynamic loads. These are unalloyed or microalloyed steels hardened mainly by the grain-boundary strengthening mechanism. Such steels require specific welding procedures, especially in terms of the heat input value. At present, there are studies of the welding influence on the change of thermomechanically processed steels’ mechanical properties, however mainly under static loading. The paper is therefore focused on the assessment of the welding effect under dynamic loading of welded joints. In the experimental part was determined the influence of five different heat input values on the change of weld fatigue life. As a result, there is both determination of five S-N curves for the double-sided fillet welds from the thermomechanically processed fine-grained steel S460MC and the quantification of the main influences reducing the fatigue life of the joint.
Influence of Interface Proximity on Precipitation Thermodynamics
Authors: Wang Kai , Weikamp Marc , Lin Mingxuan , Zimmermann Carina , Schwaiger Ruth , Prahl Ulrich , Hunkel Martin , Spatschek Robert
Published: September 2020

The formation of coherent precipitates is often accompanied by large elastic mismatch stresses, which suppress phase separation. We discuss the presence of interfaces as a mechanism for stress relaxation, which can lead to preferred zones of precipitation. In particular, we discuss the proximity of free surfaces and shear-coupled grain boundaries, for which we can obtain a substantial local energy reduction and predict the influence on the local precipitation thermodynamics. The latter case is accompanied by morphological changes of the grain boundary, which are less suitable for large-scale descriptions. For that purpose, we develop an effective description through an elastic softening inside the grain boundary and map the microscopic grain boundary relaxation to a mesoscopic elastic and phase field model, which also allows generalizing the description to multi-phase situations.
Experimental and Numerical Study of Mechanical Behavior of Welded Steel Plate Joints
Authors: Ma Hongwei , Zheng Hao , Zhang Wei , Tang Zhanzhan , Lui Eric M.
Published: September 2020

This paper describes a study of welded steel plate joints using experimental and numerical methods. The objectives of this study are to observe the mechanical behavior of welded plate joints under monotonic and cyclic loads, identify their damage degradation processes, and provide useful test data for future damage analysis of beam-column connections in steel frame structures. Six specimens were designed, of which three were tested under monotonic loads, and the other three were tested under cyclic loads. The test setup consisted of three plates arranged in a cruciform and connected by two groove welds. The monotonic and cyclic loads were applied to the free end of the two outstanding plates, inducing a pulling force on the welded joint. Because the only element studied in the present work is the weld, the sizes of the three plates were kept constant. The responses of these welded plate joints are discussed in terms of their experimentally and numerically obtained mechanical parameters, hysteretic behavior, strain variations, stiffness degradation, damage process, and failure modes. The results show that the energy damage model outperforms the displacement damage model in terms of indicating the degree of damage. Furthermore, if designed according to code, all these welded plate joints perform satisfactorily.
Modelling of Grain Size Evolution with Different Approaches via FEM When Hard Machining of AISI 4140
Authors: Tekkaya Berk , Meurer Markus , Münstermann Sebastian
Published: September 2020

Thermo-mechanical loads during hard turning lead to the formation of so-called White Layers on the machined surface. Characterized by a very fine microstructure and high hardness, White Layers have a negative effect on the fatigue life of a component. The fundamental mechanism for the White Layer formation is the dynamic recrystallization (DRX). Therefore, in the current work, two different DRX models, Helmholtz free energy and Zener-Hollomon, are implemented into Abaqus/Explicit to predict the thickness of the White Layer when hard turning quenched/tempered AISI 4140 and the results are compared with each other. For the simulation of the machining process a Finite Element Method (FEM) model based on the Coupled-Eulerian-Lagrangian (CEL) method is built up. Although both DRX models achieved a very good match between predicted and measured White Layer thickness and grain size evolution on the workpiece rim zone, the Zener-Hollomon model produced more closer agreement.
Optimum Soft Magnetic Properties of the FeSiBNbCu Alloy Achieved by Heat Treatment and Tailoring B/Si Ratio
Authors: Han Jonghee , Kwon Seoyeon , Sohn Sungwoo , Schroers Jan , Choi-Yim Haein
Published: September 2020

To increase the saturation magnetization (Ms) of commercially available soft magnetic Finemet alloys to the level comparable to that of Si-steel and Fe-based nanocrystalline alloys such as Nanoperm, Nanomet, the B or Si content in combination with annealing heat treatment was tailored. The ribbons of Fe95−xSi1BxNb3Cu1 (x = 11, 12, 13) and Fe87−xSixB9Nb3Cu1 (x = 6, 8, 10) were prepared by melt-spinning and annealed at different temperatures to develop nanocrystalline microstructure optimizing the soft magnetic properties. The magnetic properties of the as-spun and annealed ribbons were measured using a vibrating sample magnetometer and AC B-H loop tracer to acquire Ms of above 1.4 T in all as-spun ribbons. Among the alloys, Fe84Si1B11Nb3Cu1 annealed at 545 °C showed the highest Ms of 2 T, which exceeds that of the conventional Finemet and other Fe-based nanocrystalline alloys.
5-CQA and Mangiferin, Two Leaf Biomarkers of Adaptation to Full Sun or Shade Conditions in Coffea arabica L.
Authors: Duangsodsri Teerarat , Villain Luc , Vestalys Ialy Rojo , Michalet Serge , Abdallah Cécile , Breitler Jean-Christophe , Bordeaux Mélanie , Villegas Andres Mauricio , Raherimandimby Marson , Legendre Laurent , Etienne Hervé , Bertrand Benoît , Campa Claudine
Published: September 2020

Phenolic compounds are involved in plant response to environmental conditions and are highly present in leaves of Coffea arabica L., originally an understory shrub. To increase knowledge of C. arabica leaf phenolic compounds and their patterns in adaptation to light intensity, mature leaves of Ethiopian wild accessions, American pure lines and their relative F1 hybrids were sampled in full sun or under 50% shade field plots in Mexico and at two contrasting elevations in Nicaragua and Colombia. Twenty-one phenolic compounds were identified by LC-DAD-MS2 and sixteen were quantified by HPLC-DAD. Four of them appeared to be involved in C. arabica response to light intensity. They were consistently more accumulated in full sun, presenting a stable ratio of leaf content in the sun vs. shade for all the studied genotypes: 1.6 for 5-CQA, F-dihex and mangiferin and 2.8 for rutin. Moreover, 5-CQA and mangiferin contents, in full sun and shade, allowed for differentiating the two genetic groups of Ethiopian wild accessions (higher contents) vs. cultivated American pure lines. They appear, therefore, to be potential biomarkers of adaptation of C. arabica to light intensity for breeding programs. We hypothesize that low 5-CQA and mangiferin leaf contents should be searched for adaptation to full-sun cropping systems and high contents used for agroforestry systems.
Neuronal Lipoprotein Lipase Deficiency Alters Neuronal Function and Hepatic Metabolism
Authors: Bruce Kimberley D. , Dobrinskikh Evgenia , Wang Hong , Rudenko Ivan , Gao Hong , Libby Andrew E. , Gorkhali Sachi , Yu Tian , Zsombok Andrea , Eckel Robert H.
Published: September 2020

The autonomic regulation of hepatic metabolism offers a novel target for the treatment of non-alcoholic fatty liver disease (NAFLD). However, the molecular characteristics of neurons that regulate the brain-liver axis remain unclear. Since mice lacking neuronal lipoprotein lipase (LPL) develop perturbations in neuronal lipid-sensing and systemic energy balance, we reasoned that LPL might be a component of pre-autonomic neurons involved in the regulation of hepatic metabolism. Here, we show that, despite obesity, mice with reduced neuronal LPL (NEXCreLPLflox (LPL KD)) show improved glucose tolerance and reduced hepatic lipid accumulation with aging compared to wilt type (WT) controls (LPLflox). To determine the effect of LPL deficiency on neuronal physiology, liver-related neurons were identified in the paraventricular nucleus (PVN) of the hypothalamus using the transsynaptic retrograde tracer PRV-152. Patch-clamp studies revealed reduced inhibitory post-synaptic currents in liver-related neurons of LPL KD mice. Fluorescence lifetime imaging microscopy (FLIM) was used to visualize metabolic changes in LPL-depleted neurons. Quantification of free vs. bound nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) revealed increased glucose utilization and TCA cycle flux in LPL-depleted neurons compared to controls. Global metabolomics from hypothalamic cell lines either deficient in or over-expressing LPL recapitulated these findings. Our data suggest that LPL is a novel feature of liver-related preautonomic neurons in the PVN. Moreover, LPL loss is sufficient to cause changes in neuronal substrate utilization and function, which may precede changes in hepatic metabolism.
Comparison of Acoustic Streaming Flow Patterns Induced by Solid, Liquid and Gas Obstructions
Authors: Lu Hsin-Fu , Tien Wei-Hsin
Published: September 2020

In this study, acoustic streaming flows inside micro-channels induced by three different types of obstruction—gaseous bubble, liquid droplet and solid bulge—are compared and investigated experimentally by particle tracking velocimetry (PTV) and numerically using the finite element method (FEM). The micro-channels are made by poly(dimethylsiloxane) (PDMS) using soft lithography with low-cost micro-machined mold. The characteristic dimensions of the media are 0.2 mm in diameter, and the oscillation generated by piezoelectric actuators has frequency of 12 kHz and input voltages of 40 V. The experimental results show that in all three obstruction types, a pair of counter-rotating vortical patterns were observed around the semi-circular obstructions. The gaseous bubble creates the strongest vortical streaming flow, which can reach a maximum of 21 mm/s, and the largest u component happens at Y/D = 0. The solid case is the weakest of the three, which can only reach 2 mm/s. The liquid droplet has the largest v components and speed at Y/D = 0.5 and Y/D = 0.6. Because of the higher density and incompressibility of liquid droplet compared to the gaseous bubble, the liquid droplet obstruction transfers the oscillation of the piezo plate most efficiently, and the induced streaming flow region and average speed are both the largest of the three. An investigation using numerical simulation shows that the differing interfacial conditions between the varying types of obstruction boundaries to the fluid may be the key factor to these differences. These results suggest that it might be more energy-efficient to design an acoustofluidic device using a liquid droplet obstruction to induce the stronger streaming flow.
Analysis of Stiffness Effect on Valve Behavior of a Reciprocating Pump Operated by Piezoelectric Elements
Authors: Woo Jangmi , Sohn Dong Kee , Ko Han Seo
Published: September 2020

This study analyzed the characteristics of a small reciprocating pump with a cantilever valve driven by a piezo actuator. Three types of valves were fabricated to investigate the effect of the valve stiffness on the pump performance and to measure the variation in the flow rate according to the frequency. The flow rate increased with the driving frequency until a certain frequency was reached, and then it started to decrease. The rise in the pressure of the pump was found to increase as the stiffness decreased. The pump performance could be clearly distinguished according to the stiffness of the valve. The observation of the valve movements revealed that the valve opening time did not change regardless of the operating frequency, but it changed with the valve stiffness. The delay in time for the outlet valve increased significantly with an increase in the frequency. It seems that the overlap of the opening time of the inlet valve and the outlet valve plays an important role in pump performance. Therefore, it is advisable to use different designs for the inlet and outlet valves, where the shape and stiffness of the valve are adjusted.
Plasmonic Optical Biosensors for Detecting C-Reactive Protein: A Review
Authors: Seok Joo Seon , Ju Heongkyu
Published: September 2020

C-reactive protein (CRP), a potent acute-phase reactant that increases rapidly in response to inflammation, tissue damage or infections, is also considered an indicator of the risk of cardiovascular diseases and neurological disorders. Recent advances in nanofabrication and nanophotonic technologies have prompted the optical plasmonic phenomena to be tailored for specific detection of human serum CRP into label-free devices. We review the CRP-specific detection platforms with high sensitivity, which feature the thin metal films for surface plasmon resonance, nano-enhancers of zero dimensional nanostructures, and metal nanoparticles for localized surface plasmon resonance. The protocols used for various types of assay reported in literature are also outlines with surface chemical pretreatment required for specific detection of CRPs on a plasmonic surface. Properties including sensitivity and detection range are described for each sensor device reviewed, while challenges faced by plasmonic CRP sensors are discussed in the conclusion, with future directions towards which research efforts need to be made.
The Modelling, Analysis, and Experimental Validation of a Novel Micro-Robot for Diagnosis of Intestinal Diseases
Authors: Han Ding , Yan Guozheng , Wang Zhiwu , Jiang Pingping , Liu Dasheng , Zhao Kai , Ma Jin
Published: September 2020

Intestinal-related diseases all around the world are increasing nowadays, and gradually become stubborn diseases threatening human health, and even lives. Diagnosis methods have attracted more and more attention. This article concerns a non-invasive way, a novel micro-robot, to diagnose intestinal diseases. This proposed micro-robot is a swallowable device, 14 mm in diameter, like a capsule. In order to make it possible for the micro-robot to move forward, backward, or anchor itself at a suspicious lesion point in the intestine with different lumen diameter sections, two key mechanisms have been proposed. One is an expanding mechanism with two-layer folded legs for anchoring. The designed expanding mechanism could realize a large variable diameter ratio, upwards of 3.43. In addition, a pair of specific annular gears instead of a traditional pinion drive is devised not only saving limited space, but also reducing energy loss. The other mechanism is a telescoping mechanism, possessing a self-locking lead screw nut system, which is used to obtain axial motion of the micro-robot. Then, the kinematics and dynamics of the micro-robot are analyzed. After that, the following experiments, including force tests and locomotion tests, are constructed. A good match is found between the theoretical results and the experimental data. Finally, in vitro experiments are performed with a prototype to verify the safety and reliability of the proposed micro-robot in porcine intestine.
The Evaluation of Clay Suitability for Geopolymer Technology
Authors: Hanzlíček Tomáš , Perná Ivana , Uličná Kateřina , Římal Václav , Štěpánková Helena
Published: September 2020

In the last 20 years, laboratory work on geopolymer technology has been confronted with the necessity to determine whether a certain clay material has the properties necessary to form genuine geopolymer when thermally treated and alkalized. The assessment of the properties of clay source materials and of the ability of the materials to form geopolymer 3D netting mainly involves the study of the aluminum transformation level during the thermal treatment of the clay. The presented study combines several classical analytical methods (chemical and mineralogical analyses, the calculation of the Hinckley index (HI) based on X-ray diffraction analyses, and the measurement of particle size distribution) for eleven samples of kaolin and kaolinitic clay of various origins and coming from different locations. The results of these methods have been compared with those of 27Al magic angle spinning nuclear magnetic resonance (MAS NMR) in solid state. Based on them, the mentioned methods could be combined for an estimation of the aluminum-ion behavior during the thermal treatment of the clay. HI calculations have shown favorable agreement in 63% of the kaolin samples studied, especially for high purity kaolin without significant impurities in the form of feldspars and/or quartz. The main aim of this work is not to replace the precise MAS–NMR analysis, but to offer an alternative evaluation method when MAS–NMR is not available.