bluuebunny/crossref_metadata_2025 huggingface dataset:
Episode-of-Care Costs for Revision Total Joint Arthroplasties by Decadal Age Groups
Authors: Fang Christopher , Pagani Nicholas , Gordon Matthew , Talmo Carl T. , Mattingly David A. , Smith Eric L.
Published: May 2021

The demand for revision total joint arthroplasties (rTJAs) is expected to increase as the age of the population continues to rise. Accurate cost data regarding hospital expenses for differing age groups are needed to deliver optimal care within value-based healthcare (VBHC) models. The aim of this study was to compare the total in-hospital costs by decadal groups following rTJA and to determine the primary drivers of the costs for these procedures. Time-driven activity-based costing (TDABC) was used to capture granular hospital costs. A total of 551 rTJAs were included in the study, with 294 sexagenarians, 198 septuagenarians, and 59 octogenarians and older. Sexagenarians had a lower ASA classification (2.3 vs. 2.4 and 2.7; p < 0.0001) and were more often privately insured (66.7% vs. 24.2% and 33.9%; p < 0.0001) as compared to septuagenarians and octogenarians and older, respectively. Sexagenarians were discharged to home at a higher rate (85.3% vs. 68.3% and 34.3%; p < 0.0001), experienced a longer operating room (OR) time (199.8 min vs. 189.7 min and 172.3 min; p = 0.0195), and had a differing overall hospital length of stay (2.8 days vs. 2.7 days and 3.6 days; p = 0.0086) compared to septuagenarians and octogenarians and older, respectively. Sexagenarians had 7% and 23% less expensive personnel costs from post-anesthesia care unit (PACU) to discharge (p < 0.0001), and 1% and 24% more expensive implant costs (p = 0.077) compared to septuagenarians and octogenarians and older, respectively. Sexagenarians had a lower total in-hospital cost for rTJAs by 0.9% compared to septuagenarians but 12% more expensive total in-hospital costs compared to octogenarians and older (p = 0.185). Multivariate linear regression showed that the implant cost (0.88389; p < 0.0001), OR time (0.12140; p < 0.0001), personnel cost from PACU through to discharge (0.11472; p = 0.0007), and rTHAs (−0.03058; p < 0.0001) to be the strongest associations with overall costs. Focusing on the implant costs and OR times to reduce costs for all age groups for rTJAs is important to provide cost-effective VBHC.
Reservoir Sizing at Draft Level of 75% of Mean Annual Flow Using Drought Magnitude Based Method on Canadian Rivers
Authors: Sharma Tribeni C. , Panu Umed S.
Published: May 2021

On a global basis, there is trend that a majority of reservoirs are sized using a draft of 75% of the mean annual flow (0.75 MAF). The reservoir volumes based on the proposed drought magnitude (DM) method and the sequent peak algorithm (SPA) at 0.75 MAF draft were compared at the annual, monthly and weekly scales using the flow sequences of 25 Canadian rivers. In our assessment, the monthly scale is adequate for such analyses. The DM method, although capable of using flow data at any time scale, has been demonstrated using monthly standardized hydrological index (SHI) sequences. The moving average (MA) smoothing of the monthly SHI sequences formed the basis in the DM method for estimating the reservoir volume through the use of the extreme number theorem, and the hypothesis that drought magnitude is equal to the product of the drought intensity and drought length. The truncation level in the SHI sequences was found as SHIo [ = (0.75 ‒ 1) µo/σo], where µo and σo are the overall mean and standard deviation of the monthly flows. The DM-based estimates for the deficit volumes and the SPA-based reservoir volumes were found comparable within an error margin of ±18%.
A Narrative Review of the Government Wheelchair Provision System in India
Authors: Gupta Shivani , Meershoek Agnes , De Witte Luc
Published: May 2021

Background and aim: India has had a wheelchair-delivery system in place for several years but its impact on users is inadequate. Therefore, this research reviews the system to examine how the right to personal mobility can be served better. Method: this paper undertakes a narrative review of the existing government-aided wheelchair provision system from the perspectives of legislation and implementing agencies, both governmental and non-governmental, through document review and key informant interviews. Results: the results indicate that all steps of the government-funded wheelchair provision system are executed by the same system. Manufacture and supply take place nationally, but wheelchair services are largely absent. Moreover, the right to access mobility devices is not upheld for all users. Conclusion: the established government-aided wheelchair provision system is inadequate in terms of coverage, design, production, supply, and wheelchair services. Therefore, there is a need to reconsider the system by increasing its coverage and creating partnerships between the government, non-governmental agencies, and private agencies to improve access.
Upgrading Nursing Students’ Foreign Language and Communication Skills: A Qualitative Inquiry of the Afterschool Enhancement Programmes
Authors: Dos Santos Luis Miguel
Published: May 2021

Learning a foreign language is not easy for many students, particularly for nursing students who need to complete their internships in the hospital. However, motivation always drives them to the foreign language classrooms. The purpose of this study was to understand the motivations and reasons behind why these nursing students decided to study Mandarin Chinese as part of their personal development and enhancement beyond the curriculum. One main question and one sub-research question were written, why would nursing students decide to take Chinese as the tool for foreign language and culture development? What and why are the reasons and motivations for nursing students to take Chinese beyond their curriculum? The qualitative case study method was employed in order to investigate 36 nursing university students in the United Kingdom. The results of this study concluded that nursing students tend to study Chinese due to personal development and career goals. School leaders, department heads, government leaders, policymakers, human resources professionals, vocational trainers, and researchers may take this study as the opportunity to reform their current human resource and education plans to offer foreign language courses to university students, members of the public, learners, and interested parties for both personal development and career enhancements.
Left-Behind Children, Parent-Child Communication and Psychological Resilience: A Structural Equation Modeling Analysis
Authors: Zhou Chi , Lv Qiaohong , Yang Nancy , Wang Feng
Published: May 2021

This study aims to examine the role of parental migration status and parent communication in the psychological and related behavioral status of left-behind children and their psychological resilience. A cross-sectional survey was conducted in Anhui Province of China, and a questionnaire survey was conducted with 1992 teens using the Chinese version of the Parent-Adolescent Communication Scale (PACS), Connor-Davidson Resilience Scale (CD-RISC), and Strength and Difficulties Questionnaires (SDQ). Compared with the never left-behind group, left behind children had relatively lower PACS, CD-RISC and SDQ scores. Absence of parents is related with poorer psychological resilience, while good parent communication is related with better psychological resilience. Better psychological resilience is related to fewer psychological problems regardless of parental migration status. Currently left-behind status demonstrated a negative influence on psychological resilience, while never left-behind status had a positive effect. Interventions are needed to enhance psychological resilience of left-behind children to prevent psychological and related behavioral problems.
Contribution of Lower Extremity Joints on Energy Absorption during Soft Landing
Authors: Tamura Akihiro , Akasaka Kiyokazu , Otsudo Takahiro
Published: May 2021

Soft landing after jumping is associated with the prevention of lower extremity injuries during sports activities in terms of the energy absorption mechanisms. In this study, the contribution of lower extremity joints during soft landing was investigated. Subjects comprised 20 healthy females. Kinetics and kinematics data were obtained during drop vertical jumps using a three-dimensional motion analysis system. Negative mechanical work values in the lower extremity joints were calculated during landing. A multiple regression analysis was performed to determine which lower extremity joints contributed more in achieving soft landing. The means of mechanical work of the hip, knee, and ankle in the sagittal plane were −0.30 ± 0.17, −0.62 ± 0.31, and −1.03 ± 0.22 J/kg, respectively. Results showed that negative mechanical work in the hip and knee is effective in achieving soft landing. These findings indicate that energy absorption in the hip and knee joints might be an important factor in achieving soft landing, whereas that in the ankle has a negative effect. Therefore, when improving soft landing techniques, we should consider energy absorption in the hip and knee via eccentric activation of the hip and knee extensors during landing.
Food Environment in and around Primary School Children’s Schools and Neighborhoods in Two Urban Settings in Kenya
Authors: Gewa Constance Awuor , Onyango Agatha Christine , Opiyo Rose Okoyo , Cheskin Lawrence , Gittelsohn Joel
Published: May 2021

We conducted a cross-sectional study to provide an overview primary school children food environment in two urban settings in Kenya. Six schools, catering to children from low-, medium- and high-income households in the cities of Nairobi and Kisumu in Kenya, participated in the study. Data on types of food places and foods offered were collected and healthy and unhealthy food availability scores calculated for each place. We utilized prevalence ratio analysis to examine associations between food availability, food place characteristics and neighborhood income levels. Altogether, 508 food places, located within 1 km of the schools and the school children’s neighborhoods were observed. Open-air market sellers and kiosks were most common. The proportion of food places with high healthy food availability was 2.2 times greater among food places in Nairobi compared to Kisumu, 1.9 times greater in food places with multiple cashpoints, 1.7 times greater in medium/large sized food places and 1.4 times greater in food places located in high income neighborhoods. These findings highlight differences in availability of healthy foods and unhealthy foods across types of food places and neighborhood income levels and inform public health interventions aimed at promoting healthy food environments in Kenya.
Phosphinotripeptidic Inhibitors of Leucylaminopeptidases
Authors: Jewgiński Michał , Haremza Kinga , de los Santos Jesús M. , Es Sbai Zouhair , Oszywa Bartosz , Pawełczak Małgorzata , Palacios Francisco , Latajka Rafał
Published: May 2021

Phosphinate pseudopeptide are analogs of peptides containing phosphinate moiety in a place of the amide bond. Due to this, the organophosphorus fragment resembles the tetrahedral transition state of the amide bond hydrolysis. Additionally, it is also capable of coordinating metal ions, for example, zinc or magnesium ions. These two properties of phosphinate pseudopeptides make them an ideal candidate for metal-related protease inhibitors. This research investigates the influence of additional residue in the P2 position on the inhibitory properties of phosphinopeptides. The synthetic strategy is proposed, based on retrosynthetic analysis. The N-C-P bond formation in the desired compounds is conveniently available from the three-component condensation of appropriate amino components, aldehydes, and hypophosphorous acid. One of the crucial synthetic steps is the careful selection of the protecting groups for all the functionals. Determination of the inhibitor activity of the obtained compounds has been done using UV-Vis spectroscopy and standard substrate L-Leu-p-nitroanilide toward the enzymes isolated from the porcine kidney (SsLAP, Sus scrofa Leucine aminopeptidase) and barley seeds (HvLAP, Hordeum vulgare Leucine aminopeptidase). An efficient procedure for the preparation of phosphinotripeptides has been performed. Activity test shown that introduction of additional residue into P2 position obtains the micromolar range inhibitors of SsLAP and HvLAP. Moreover, careful selection of the residue in the P2 position should improve its selectivity toward mammalian and plant leucyl aminopeptidases.
The Use of Silica Microparticles to Improve the Efficiency of Optical Hyperthermia (OH)
Authors: Casanova-Carvajal O. , Zeinoun M. , Urbano-Bojorge A. L. , Bacha F. , Solera Livi J. , Agudo E. , Vargas G. , Ramos M. , Martínez-Murillo R. , Serrano-Olmedo J. J.
Published: May 2021

Although optical hyperthermia could be a promising anticancer therapy, the need for high concentrations of light-absorbing metal nanoparticles and high-intensity lasers, or large exposure times, could discourage its use due to the toxicity that they could imply. In this article, we explore a possible role of silica microparticles that have high biocompatibility and that scatter light, when used in combination with conventional nanoparticles, to reduce those high concentrations of particles and/or those intense laser beams, in order to improve the biocompatibility of the overall procedure. Our underlying hypothesis is that the scattering of light caused by the microparticles would increase the optical density of the irradiated volume due to the production of multiple reflections of the incident light: the nanoparticles present in the same volume would absorb more energy from the laser than without the presence of silica particles, resulting either in higher heat production or in the need for less laser power or absorbing particles for the same required temperature rise. Testing this new optical hyperthermia procedure, based on the use of a mixture of silica and metallic particles, we have measured cell mortality in vitro experiments with murine glioma (CT-2A) and mouse osteoblastic (MC3T3-E1) cell lines. We have used gold nanorods (GNRs) that absorb light with a wavelength of 808 nm, which are conventional in optical hyperthermia, and silica microparticles spheres (hereinafter referred to as SMSs) with a diameter size to scatter the light of this wavelength. The obtained results confirm our initial hypothesis, because a high mortality rate is achieved with reduced concentrations of GNR. We found a difference in mortality between CT2A cancer cells and cells considered non-cancer MC3T3, maintaining the same conditions, which gives indications that this technique possibly improves the efficiency in the cell survival. This might be related with differences in the proliferation rate. Since the experiments were carried out in the 2D dimensions of the Petri dishes, due to sedimentation of the silica particles at the bottom, whilst light scattering is a 3D phenomenon, a large amount of the energy provided by the laser escapes outside the medium. Therefore, better results might be expected when applying this methodology in tissues, which are 3D structures, where the multiple reflections of light we believe will produce higher optical density in comparison to the conventional case of no using scattering particles. Accordingly, further studies deserve to be carried out in this line of work in order to improve the optical hyperthermia technique.
Intestinal Homeostasis under Stress Siege
Authors: Guzmán-Mejía Fabiola , Godínez-Victoria Marycarmen , Vega-Bautista Alan , Pacheco-Yépez Judith , Drago-Serrano Maria Elisa
Published: May 2021

Intestinal homeostasis encompasses a complex and balanced interplay among a wide array of components that collaborate to maintain gut barrier integrity. The appropriate function of the gut barrier requires the mucus layer, a sticky cushion of mucopolysaccharides that overlays the epithelial cell surface. Mucus plays a critical anti-inflammatory role by preventing direct contact between luminal microbiota and the surface of the epithelial cell monolayer. Moreover, mucus is enriched with pivotal effectors of intestinal immunity, such as immunoglobulin A (IgA). A fragile and delicate equilibrium that supports proper barrier function can be disturbed by stress. The impact of stress upon intestinal homeostasis results from neuroendocrine mediators of the brain-gut axis (BGA), which comprises a nervous branch that includes the enteric nervous system (ENS) and the sympathetic and parasympathetic nervous systems, as well as an endocrine branch of the hypothalamic-pituitary-adrenal axis. This review is the first to discuss the experimental animal models that address the impact of stress on components of intestinal homeostasis, with special emphasis on intestinal mucus and IgA. Basic knowledge from animal models provides the foundations of pharmacologic and immunological interventions to control disturbances associated with conditions that are exacerbated by emotional stress, such as irritable bowel syndrome.
Molecular Basis of Essentiality of Early Critical Steps in the Lipopolysaccharide Biogenesis in Escherichia coli K-12: Requirement of MsbA, Cardiolipin, LpxL, LpxM and GcvB
Authors: Gorzelak Patrycja , Klein Gracjana , Raina Satish
Published: May 2021

To identify the physiological factors that limit the growth of Escherichia coli K-12 strains synthesizing minimal lipopolysaccharide (LPS), we describe the first construction of strains devoid of the entire waa locus and concomitantly lacking all three acyltransferases (LpxL/LpxM/LpxP), synthesizing minimal lipid IVA derivatives with a restricted ability to grow at around 21 °C. Suppressors restoring growth up to 37 °C of Δ(gmhD-waaA) identified two independent single-amino-acid substitutions—P50S and R310S—in the LPS flippase MsbA. Interestingly, the cardiolipin synthase-encoding gene clsA was found to be essential for the growth of ΔlpxLMP, ΔlpxL, ΔwaaA, and Δ(gmhD-waaA) bacteria, with a conditional lethal phenotype of Δ(clsA lpxM), which could be overcome by suppressor mutations in MsbA. Suppressor mutations basS A20D or basR G53V, causing a constitutive incorporation of phosphoethanolamine (P-EtN) in the lipid A, could abolish the Ca++ sensitivity of Δ(waaC eptB), thereby compensating for P-EtN absence on the second Kdo. A single-amino-acid OppA S273G substitution is shown to overcome the synthetic lethality of Δ(waaC surA) bacteria, consistent with the chaperone-like function of the OppA oligopeptide-binding protein. Furthermore, overexpression of GcvB sRNA was found to repress the accumulation of LpxC and suppress the lethality of LapAB absence. Thus, this study identifies new and limiting factors in regulating LPS biosynthesis.
Effect of Ingestion Exposure of Selected Insecticides on Coccinella septempunctata and Harmonia axyridis (Coleoptera: Coccinellidae)
Authors: Twardowski Jacek Piotr , Hurej Michał , Twardowska Kamila
Published: May 2021

The sensitivity to thiacloprid and lambda-cyhalothrin ingested from prey organisms was studied in Coccinella septempunctata and Harmonia axyridis, since the effect of ingestion exposure to these insecticides is unknown in these species. All developmental stages of the ladybirds were fed on Acyrthosiphon pisum treated with half or full field rate of the insecticides. Almost all instars were killed within 3 h of the start of ingestion of lambda-cyhalotrin-treated prey. The action of thiacloprid was more extended in time in both coccinellids and more variable between their instars. Reducing the field rate of lambda-cyhalothrin seems to have no practical value for the survival of either of the coccinellid species. Contrastingly, using half instead of the full field rate of thiacloprid may enhance the chances of survival in L1, L2, and L4 larvae of both species. Of all developmental stages tested, the survival dynamics of the adults of either species are closest to one another, whereas the apparent difference in the species response to the dose rate of thiacloprid was found in the L4 stage.
Entangling the Enemy: Ecological, Systematic, and Medical Implications of Dermestid Beetle Hastisetae
Authors: Ruzzier Enrico , Kadej Marcin , Di Giulio Andrea , Battisti Andrea
Published: May 2021

Hastisetae are modified setae typical of Dermestidae Megatominae and are a primary defensive tool of both larvae and pupae against invertebrates and possibly vertebrates. Given their unique morphological features, hastisetae have recently been suggested as an additional character useful for larvae identification and possible source of information to clarify the systematics of Megatominae. Hastisetae are also recognized as important contaminants of stored products, work and living environment; in particular, the exposure to hastisetae seems to cause allergic reactions and the insurgence of skin rashes, asthma, conjunctivitis, and digestive system inflammation in humans. Starting from these basic concepts, the present paper provides a detailed description of the hastisetae of some Megatominae. Fine morphology of external and internal microstructures of the hastisetae is shown and compared at the genus level. The insertion on integument, the pedicel, the shaft, and the apical head are illustrated in detail, and the first observations of active defensive behavior based on hastisetae are recorded and presented. Possible implications to the systematics of skin beetles are proposed based on the results of the study.
The Impact of Climate Change on Agricultural Insect Pests
Authors: Skendžić Sandra , Zovko Monika , Živković Ivana Pajač , Lešić Vinko , Lemić Darija
Published: May 2021

Climate change and global warming are of great concern to agriculture worldwide and are among the most discussed issues in today’s society. Climate parameters such as increased temperatures, rising atmospheric CO2 levels, and changing precipitation patterns have significant impacts on agricultural production and on agricultural insect pests. Changes in climate can affect insect pests in several ways. They can result in an expansion of their geographic distribution, increased survival during overwintering, increased number of generations, altered synchrony between plants and pests, altered interspecific interaction, increased risk of invasion by migratory pests, increased incidence of insect-transmitted plant diseases, and reduced effectiveness of biological control, especially natural enemies. As a result, there is a serious risk of crop economic losses, as well as a challenge to human food security. As a major driver of pest population dynamics, climate change will require adaptive management strategies to deal with the changing status of pests. Several priorities can be identified for future research on the effects of climatic changes on agricultural insect pests. These include modified integrated pest management tactics, monitoring climate and pest populations, and the use of modelling prediction tools.
In Vitro Activity of Novel Antifungal Olorofim against Filamentous Fungi and Comparison to Eight Other Antifungal Agents
Authors: Georgacopoulos Ourania , Nunnally Natalie , Ransom Eric , Law Derek , Birch Mike , Lockhart Shawn , Berkow Elizabeth
Published: May 2021

Olorofim is a novel antifungal drug that belongs to the orotomide drug class which inhibits fungal dihydroorotate dehydrogenase (DHODH), thus halting pyrimidine biosynthesis and ultimately DNA synthesis, cell growth and division. It is being developed at a time when many invasive fungal infections exhibit antifungal resistance or have limited treatment options. The goal of this study was to evaluate the in vitro effectiveness of olorofim against a large collection of recently isolated, clinically relevant American mold isolates. In vitro antifungal activity was determined for 246 azole-susceptible Aspergillus fumigatus isolates, five A. fumigatus with TR34/L98H-mediated resistance, 19 Rhizopus species isolates, 21 Fusarium species isolates, and one isolate each of six other species of molds. Olorofim minimum inhibitory concentrations (MICs) were compared to antifungal susceptibility testing profiles for amphotericin B, anidulafungin, caspofungin, isavuconazole, itraconazole, micafungin, posaconazole, and voriconazole. Olorofim MICs were significantly lower than those of the echinocandin and azole drug classes and amphotericin B. A. fumigatus wild type and resistant isolates shared the same MIC50 = 0.008 μg/mL. In non-Aspergillus susceptible isolates (MIC ≤ 2 μg/mL), the geometric mean (GM) MIC to olorofim was 0.54 μg/mL with a range of 0.015–2 μg/mL. Olorofim had no antifungal activity (MIC ≥ 2 μg/mL) against 10% of the collection (31 in 297), including some isolates from Rhizopus spp. and Fusarium spp. Olorofim showed promising activity against A. fumigatus and other molds regardless of acquired azole resistance.
Carbonation Reaction Mechanisms of Portlandite Predicted from Enhanced Ab Initio Molecular Dynamics Simulations
Authors: Mutisya Sylvia M. , Kalinichev Andrey G.
Published: May 2021

Geological carbon capture and sequestration (CCS) is a promising technology for curbing the global warming crisis by reduction of the overall carbon footprint. Degradation of cement wellbore casings due to carbonation reactions in the underground CO2 storage environment is one of the central issues in assessing the long-term success of the CCS operations. However, the complexity of hydrated cement coupled with extreme subsurface environmental conditions makes it difficult to understand the carbonation reaction mechanisms leading to the loss of well integrity. In this work, we use biased ab initio molecular dynamics (AIMD) simulations to explore the reactivity of supercritical CO2 with the basal and edge surfaces of a model hydrated cement phase—portlandite—in dry scCO2 and water-rich conditions. Our simulations show that in dry scCO2 conditions, the undercoordinated edge surfaces of portlandite experience a fast barrierless reaction with CO2, while the fully hydroxylated basal surfaces suppress the formation of carbonate ions, resulting in a higher reactivity barrier. We deduce that the rate-limiting step in scCO2 conditions is the formation of the surface carbonate barrier which controls the diffusion of CO2 through the layer. The presence of water hinders direct interaction of CO2 with portlandite as H2O molecules form well-structured surface layers. In the water-rich environment, CO2 undergoes a concerted reaction with H2O and surface hydroxyl groups to form bicarbonate complexes. We relate the variation of the free-energy barriers in the formation of the bicarbonate complexes to the structure of the water layer at the interface which is, in turn, dictated by the surface chemistry and the degree of nanoconfinement.
Contribution of Nano-Zero-Valent Iron and Arbuscular Mycorrhizal Fungi to Phytoremediation of Heavy Metal-Contaminated Soil
Authors: Cheng Peng , Zhang Shuqi , Wang Quanlong , Feng Xueying , Zhang Shuwu , Sun Yuhuan , Wang Fayuan
Published: May 2021

Soil pollution with heavy metals has attracted increasing concern, which calls for the development of new remediation strategies. The combination of physical, chemical, and biological techniques can achieve more efficient remediation. However, few studies have focused on whether nanomaterials and beneficial microbes can be jointly used to facilitate phytoremediation. Therefore, we studied the role of nano-zero-valent iron (nZVI) and arbuscular mycorrhizal (AM) fungi in the phytoremediation of an acidic soil polluted with Cd, Pb and Zn, using sweet sorghum. X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS), and mapping analyses were conducted to explore the mechanisms of metal immobilization by nZVI. The results showed that although both bare nZVI (B-nZVI) and starch-stabilized nZVI (S-nZVI) inhibited root mycorrhizal colonization, Acaulospora mellea ZZ successfully colonized the plant roots. AM inoculation significantly reduced the concentrations of DTPA-Cd, -Pb, and -Zn in soil, and the concentrations of Cd, Pb, and Zn in plants, indicating that AM fungi substantially facilitated heavy metal immobilization. Both B-nZVI and S-nZVI, ranging from 50 mg/kg to 1000 mg/kg, did not impede plant growth, and generally enhanced the phytoextraction of heavy metals. XRD, EDS and mapping analyses showed that S-nZVI was more susceptible to oxidation than B-nZVI, and thus had more effective immobilization effects on heavy metals. Low concentrations of nZVI (e.g., 100 mg/kg) and AM inoculation had synergistic effects on heavy metal immobilization, reducing the concentrations of Pb and Cd in roots and enhancing root Zn accumulation. In conclusion, our results showed that AM inoculation was effective in immobilizing heavy metals, whereas nZVI had a low phytotoxicity, and they could jointly contribute to the phytoremediation of heavy metal-contaminated soils with sweet sorghum.
Elucidating the Functions of Non-Coding RNAs from the Perspective of RNA Modifications
Authors: Garikipati Venkata Naga Srikanth , Uchida Shizuka
Published: May 2021

It is now commonly accepted that most of the mammalian genome is transcribed as RNA, yet less than 2% of such RNA encode for proteins. A majority of transcribed RNA exists as non-protein-coding RNAs (ncRNAs) with various functions. Because of the lack of sequence homologies among most ncRNAs species, it is difficult to infer the potential functions of ncRNAs by examining sequence patterns, such as catalytic domains, as in the case of proteins. Added to the existing complexity of predicting the functions of the ever-growing number of ncRNAs, increasing evidence suggests that various enzymes modify ncRNAs (e.g., ADARs, METTL3, and METTL14), which has opened up a new field of study called epitranscriptomics. Here, we examine the current status of ncRNA research from the perspective of epitranscriptomics.
Pyrolysis Kinetic Behaviour of Glass Fibre-Reinforced Epoxy Resin Composites Using Linear and Nonlinear Isoconversional Methods
Authors: Yousef Samy , Eimontas Justas , Striūgas Nerijus , Praspaliauskas Marius , Abdelnaby Mohammed Ali
Published: May 2021

Due to the increasing demand for glass fibre-reinforced epoxy resin composites (GFRC), huge amounts of GFRC waste are produced annually in different sizes and shapes, which may affect its thermal and chemical decomposition using pyrolysis technology. In this context, this research aims to study the effect of mechanical pre-treatment on the pyrolysis behaviour of GFRC and its pyrolysis kinetic. The experiments were started with the fabrication of GFRC panels using the vacuum-assisted resin transfer method followed by crushing the prepared panels using ball milling, thus preparing the milled GFRC with uniform shape and size. The elemental, proximate, and morphology properties of the panels and milled GFRC were studied. The thermal and chemical decomposition of the milled GFRC was studied using thermogravimetric coupled with Fourier-transform infrared spectroscopy (TG-FTIR) at different heating rates. Meanwhile, the volatile products were examined using TG coupled with gas chromatography–mass spectrometry (GC-MS). The TG-FTIR and TG-GC-MS experiments were performed separately. Linear (Kissinger–Akahira–Sunose (KAS), Flynn–Wall–Ozawa (FWO), and Friedman) and nonlinear (Vyazovkin and Cai) isoconversional methods were used to determine the pyrolysis kinetic of the milled GFRC based on thermogravimetry and differential thermal gravimetry (TG/DTG). In addition, the TG/DTG data of the milled GFRC were fitting using the distributed activation energy model and the independent parallel reactions kinetic model. The TG results showed that GFRC can decompose in three stages, and the main decomposition is located in the range 256–500 °C. On the other hand, aromatic benzene and a C-H bond were the major functional groups in the released volatile components in FTIR spectra, while phenol (27%), phenol,4-(1-methylethyl) (40%), and p-isopropenylphenol (34%) were the major compounds in GC-MS analysis. Whereas, the kinetic results showed that both isoconversional methods can be used to determine activation energies, which were estimated 165 KJ/mol (KAS), 193 KJ/mol (FWO), 180 KJ/mol (Friedman), 177 KJ/mol (Vyazovkin), and 174 KJ/mol (Cai).
Farmers’ Perceptions of Commercial Insect-Based Feed for Sustainable Livestock Production in Kenya
Authors: Okello Afrika Onguko , Nzuma Jonathan Makau , Otieno David Jakinda , Kidoido Michael , Tanga Chrysantus Mbi
Published: May 2021

The utilization of insect-based feeds (IBF) as an alternative protein source is increasingly gaining momentum worldwide owing to recent concerns over the impact of food systems on the environment. However, its large-scale adoption will depend on farmers’ acceptance of its key qualities. This study evaluates farmer’s perceptions of commercial IBF products and assesses the factors that would influence its adoption. It employs principal component analysis (PCA) to develop perception indices that are subsequently used in multiple regression analysis of survey data collected from a sample of 310 farmers. Over 90% of the farmers were ready and willing to use IBF. The PCA identified feed performance, social acceptability of the use of insects in feed formulation, feed versatility and marketability of livestock products reared on IBF as the key attributes that would inform farmers’ purchase decisions. Awareness of IBF attributes, group membership, off-farm income, wealth status and education significantly influenced farmers’ perceptions of IBF. Interventions such as experimental demonstrations that increase farmers’ technical knowledge on the productivity of livestock fed on IBF are crucial to reducing farmers’ uncertainties towards acceptability of IBF. Public partnerships with resource-endowed farmers and farmer groups are recommended to improve knowledge sharing on IBF.
What Could Entrepreneurial Vision Do for Sustainable Development? Explore the Cross-Level Impact of Organizational Members’ Green Shared Vision on Green Creativity
Authors: Fang Wenchang , Wu Tzong-Hann , Chang Tai-Wei , Hung Cheng-Ze
Published: May 2021

Entrepreneurial vision promotes innovation, which is a critical factor contributing to the success of an organization in the globalized world. However, the increasing awareness regarding sustainable development among people worldwide has not induced an increase in organizations’ concerns about environmental issues. An organization’s green shared vision (GSV) states its environmentally friendly philosophy to its members; however, few studies have examined GSV from a cross-level perspective. To fill this research gap, this study examined the influences of GSV on the psychological processes, attitudes, and behaviors of organization members. This study focuses on individuals’ psychological processes to explore the relationships among an organization’s GSV, green product psychological ownership (GPPO), proactive green innovation (PGI), reactive green innovation (RGI), and green creativity (GC). This study’s participants are employees from the research and development, design, marketing, and sales departments of small and medium-sized enterprises in Taiwan. The results obtained by surveying 575 employees in 72 teams indicate that team-level GSV has a positive and significant relationship with GPPO and GC at the individual level. In addition, our research results indicate the existence of mediating effects in the relationships between GPPO and GC and between PGI and RGI. Thus, GSV, GPPO, PGI and RGI greatly influence GC. Consequently, an organization should develop GSV to promote GPPO among its members to facilitate green innovation and enhance the GC of its members.
Profitability Assessment of Residential Photovoltaic Battery Systems in Japan Using Electric Power Big Data
Authors: Honda Tomonori , Ozawa Akito , Wakamatsu Hiroko
Published: May 2021

Residential photovoltaic (PV) battery systems are key technology in the design of low-carbon and resilient energy systems; however, limited research has assessed their profitability. This study aims to evaluate the economic performance of PV battery systems for end-users. The evaluation takes geological, technological, and socio-economic factors into consideration, thereby making the evaluation more comprehensive. We used PV power generation data and power consumption data of more than 40,000 all-electric houses in Japan. We performed scenario analyses with a sensitivity analysis. The results showed that residential PV battery systems were highly profitable when their storage battery operation modes were appropriately utilized at the end of the purchase period for solar power generation in Japan’s feed-in-tariff (FIT) scheme. The profitability, however, varied across regions. The results also indicated that the PV self-consumption rate was more than 50% when charging the battery with surplus power. The results of the sensitivity analysis suggested that the unit prices of grid electricity and the purchasing price of surplus power after the FIT scheme had a significant effect on the profitability of residential PV battery systems.
Promoting Sustainable Creativity: An Empirical Study on the Application of Mind Mapping Tools in Graphic Design Education
Authors: Dong Yenan , Zhu Shangshang , Li Wenjie
Published: May 2021

The development of creative thinking and creative problem solving is an important part of modern sustainable education. In teaching graphic design, educators should ensure the cultivation of sustainable creativity among students. Creative thinking and program development can be facilitated with the help of effective thinking tools. Among the various thinking tools, mind maps represent an easy-to-use visual diagram-based divergent thinking tool. However, few studies have examined the impact of mind maps on sustainable creativity in the design discipline. Similarly, few empirical studies have been conducted to analyse deeply the functional structure of and a graphic design education based on mind mapping. This study aims to examine the relation between students’ use of mind mapping tools and sustainable creativity in implementing mind mapping-based graphic design activities with a sample size of 35 first-year design students. Through an empirical experiment, each student was asked to create a mind map and generate a new graphic design work based on the given experimental materials. The results showed that the mind mapping tool plays an important role in the pre-conceptualization phase of the design process. This tool can effectively help designers develop creative ideas, stimulate their minds, and help them maintain their creative energy and have a sustainable and innovative creative ability.
Collaborative Behavior in Relational Contracting Projects in Hong Kong—A Contractor’s Perspective
Authors: Memon Shoeb Ahmed , Rowlinson Steve , Sunindijo Riza Yosia , Zahoor Hafiz
Published: May 2021

The construction industry in Hong Kong has adopted relational contracting (RC) as a way forward to address frequent conflicts in construction projects and to promote sustainable development. Despite this effort, adversarial behavior of project team members is still prevalent, stemming from conflicting agendas, which hinders the successful implementation of RC. There is a need to improve collaborative attitudes and behavior among project team members in RC projects, but there is still a lack of understanding of factors that can promote this inter-organizational collaboration. Therefore, using the theory of planned behavior, this research investigates factors that form relational attitudes, collaborative intentions, and collaborative behavior, and their relationships in RC projects in Hong Kong. Quantitative data were collected from experienced practitioners in RC projects and were analyzed using structural equation modeling (SEM). The results reveal that senior management commitment and relational norms are needed to nurture relational attitudes, which in turn influence the development of collaborative intentions. These intentions can eventually promote collaborative behavior, which is expressed by teamwork, affective trust, and extra-role behavior (striving beyond roles to maintain collaboration). The findings advance knowledge and contribute to practice by providing a structured process to nurture collaboration in RC projects for sustainable development.
Effectiveness Assessment of CAD Simulation in Complex Orthopedic Surgery Practices
Authors: Frizziero Leonardo , Pagliari Curzio , Donnici Giampiero , Liverani Alfredo , Santi Gian Maria , Papaleo Paola , Napolitano Francesca , Leon-Cardenas Christian , Trisolino Giovanni , Zarantonello Paola , Di Gennaro Giovanni Luigi , Stilli Stefano , Stallone Stefano
Published: May 2021

This experimental study defines the usage of a computer-aided surgical simulation process that is effective, safe, user-friendly, and low-cost, that achieves a detailed and realistic representation of the anatomical region of interest. The chosen tools for this purpose are state-of-the-art Computer Aided Design (CAD) software for mechanical design, and are the fundamental application dedicated to parametric modeling. These tools support different work environments, each one is for a specific type of modeling, and they allow the simulation of surgery. The result will be a faithful representation of the anatomical part both before and after the surgical procedure, screening all the intermediate phases. The doctor will assess different lines of action according to the results, then he will communicate them to the engineer who, consequently, will correct the antisymmetric issue and regenerate the model. Exact measurements of the mutual positions of the various components, skeletal and synthetic, can be achieved; all the osteosynthesis tools, necessary for the surgeon, can be included in the project according to different types of fracture to perfectly match the morphology of the bone to be treated. The method has been tested on seven clinical cases of different complexity and nature and the results of the simulations have been found to be of great effectiveness in the phase of diagnosis and of preoperative planning for the doctors and surgeons; therefore, allowing a lower risk medical operation with a better outcome. This work delivers experimental results in line with theoretical research findings in detail; moreover, full experimental and/or methodical details are provided, so that outcomes could be obtained.
Cholera Hot-Spots and Contextual Factors in Burundi, Planning for Elimination
Authors: Debes Amanda K. , Shaffer Allison M. , Ndikumana Thaddee , Liesse Iteka , Ribaira Eric , Djumo Clement , Ali Mohammad , Sack David A.
Published: May 2021

The Republic of Burundi first reported cholera cases in 1978 and outbreaks have been occurring nearly every year since then. From 2008–2020, 6949 cases and 43 deaths were officially reported. To evaluate Burundi’s potential to eliminate cholera, we identified hotspots using cholera incidence and disease persistence as suggested by the Global Task Force for Cholera Control. The mean annual incidence for each district that reported cholera ranged from 0.29 to 563.14 cases per 100,000 population per year from 2014–2020. Ten of 12 Health Districts which recorded cholera cases reported a mean annual incidence ≥5 per 100,000 for this time period. Cholera cases occur during the second half of the year in the areas near Lake Tanganyika and along the Ruzizi River, with the highest risk district being Bujumbura Centre. Additional research is needed to understand the role of Lake Tanganyika; risks associated with fishing; migration patterns; and other factors that may explain cholera’s seasonality. Due to the consistent epidemiological pattern and the relatively small area affected by cholera, control and elimination are feasible with an integrated program of campaigns using oral cholera vaccine over the short term and community-based interventions including WASH activities for sustained control.
Feedback from Active Galactic Nuclei in Galaxy Groups
Authors: Eckert Dominique , Gaspari Massimo , Gastaldello Fabio , Le Brun Amandine M. C. , O’Sullivan Ewan
Published: May 2021

The co-evolution between supermassive black holes and their environment is most directly traced by the hot atmospheres of dark matter halos. The cooling of the hot atmosphere supplies the central regions with fresh gas, igniting active galactic nuclei (AGN) with long duty cycles. Outflows from the central engine tightly couple with the surrounding gaseous medium and provide the dominant heating source preventing runaway cooling by carving cavities and driving shocks across the medium. The AGN feedback loop is a key feature of all modern galaxy evolution models. Here, we review our knowledge of the AGN feedback process in the specific context of galaxy groups. Galaxy groups are uniquely suited to constrain the mechanisms governing the cooling–heating balance. Unlike in more massive halos, the energy that is supplied by the central AGN to the hot intragroup medium can exceed the gravitational binding energy of halo gas particles. We report on the state-of-the-art in observations of the feedback phenomenon and in theoretical models of the heating-cooling balance in galaxy groups. We also describe how our knowledge of the AGN feedback process impacts galaxy evolution models and large-scale baryon distributions. Finally, we discuss how new instrumentation will answer key open questions on the topic.
Mutation in a SARS-CoV-2 Haplotype from Sub-Antarctic Chile Reveals New Insights into the Spike’s Dynamics
Authors: González-Puelma Jorge , Aldridge Jacqueline , Montes de Oca Marco , Pinto Mónica , Uribe-Paredes Roberto , Fernández-Goycoolea José , Alvarez-Saravia Diego , Álvarez Hermy , Encina Gonzalo , Weitzel Thomas , Muñoz Rodrigo , Olivera-Nappa Álvaro , Pantano Sergio , Navarrete Marcelo A.
Published: May 2021

The emergence of SARS-CoV-2 variants, as observed with the D614G spike protein mutant and, more recently, with B.1.1.7 (501Y.V1), B.1.351 (501Y.V2) and B.1.1.28.1 (P.1) lineages, represent a continuous threat and might lead to strains of higher infectivity and/or virulence. We report on the occurrence of a SARS-CoV-2 haplotype with nine mutations including D614G/T307I double-mutation of the spike. This variant expanded and completely replaced previous lineages within a short period in the subantarctic Magallanes Region, southern Chile. The rapid lineage shift was accompanied by a significant increase of cases, resulting in one of the highest incidence rates worldwide. Comparative coarse-grained molecular dynamic simulations indicated that T307I and D614G belong to a previously unrecognized dynamic domain, interfering with the mobility of the receptor binding domain of the spike. The T307I mutation showed a synergistic effect with the D614G. Continuous surveillance of new mutations and molecular analyses of such variations are important tools to understand the molecular mechanisms defining infectivity and virulence of current and future SARS-CoV-2 strains.