bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphological and phylogenetic analyses reveal two new species of Candolleomyces (Psathyrellaceae) from India
Authors: NAYANA P.K. , PRADEEP C.K.
Published: July 2024

Our continuous investigation into the diversity of psathyrelloid mushrooms in Kerala State, India, has led us to discover two new species of Candolleomyces viz., C. niveosquamosus and C. rubrobrunneus. Comprehensive morphological evaluations and multigene phylogenetic analyses involving nrITS and nrLSU served as the basis for describing these two new species. Comprehensive descriptions, photographs, and a discussion with related species are provided.
Impatiens uncata (Balsaminaceae), a new species from Hunan, China
Authors: QIU KAN-YING , ZHOU JIAN-JUN , XIANG JIAN-FENG , LIU ANG , FEI DONG-BO , LIU SONG , YU XUN-LIN , CONG YI-YAN
Published: July 2024

Impatiens uncata, a new species from Ningyuan County, Hunan, is described and illustrated. The new species is superficially similar to I. huangyanensis in having racemose and 2-flowered (rarely many-flowered) inflorescences, fusiform capsule and ellipsoid seed, but is distinguishable from the latter by its obviously taller height, leaves with ovate or ovate-lanceolate blades and longer petioles, purple flowers, longer bracts, broadly ovate-rounded lateral sepals, white broadly funnelform lower sepal, 2-lobed spur with a swollen apex, purplish dorsal petal, white basal lobes, and bigger distal lobes with a retuse apex.
Taxonomic study of wasp species in the Polybia (Myrapetra) occidentalis group morphologically related to P. scrobalis and P. flavifrons, with description of a new species (Hymenoptera, Vespidae, Polistinae)
Authors: AMORIM DANIELLE C. DE A. , JUNIOR JOSÉ N. A. DOS SANTOS , FILHO FERNANDO DA S. CARVALHO– , SILVEIRA ORLANDO T. , CARPENTER JAMES M.
Published: July 2024

Polybia is a genus of neotropical swarming wasps, and the largest within the Epiponini (Vespidae). The genus is dominant in the Neotropics, with more than 59 described species and eleven recognized subgenera. Myrapetra is the largest subgenus, currently with twenty-four valid species and fourteen subspecies, most of them allocated in the “P. occidentalis species-group”. Several species within Myrapetra are poorly characterized, with imprecise limits, and subspecies are largely based on color, that is highly variable feature, even among nest mates. This study is about those species of the P. occidentalis species-group which are morphologically more distinct from P. occidentalis (and its more similar neighbors), which we refer to as the species related to P. scrobalis and P. flavifrons. We describe a new species (P. rosalinae Amorim & Santos, sp. nov.) and for three subspecies we recognize the need to treat as distinct species (P. brevitarsus Richards, stat. nov.; P. hecuba Richards, stat. nov.; and P. surinama Richards, stat. nov.). In addition, we describe the males of two species and provide the first data (description and photos) on male genitalia of four taxa. P. dimorpha Richards, previously an isolated species within Myrapetra is treated as a member of the P. occidentalis species-group. Additionally, we provide a new identification key for the studied species.
Description of Branchiobdella turkestanensis n. sp. (Annelida: Clitellata) following a reexamination of specimens in the Natural History Museum Vienna, Austria
Authors: GELDER STUART R.
Published: July 2024

A new species of branchiobdellidan annelid, Branchiobdella turkestanensis, is described having been removed from a specimen of Pontastacus kessleri (Schimkewitsch, 1886), reportedly from Turkestan but collected from the headwaters of the Syr Darya which is in the Fergana Valley, Uzbekistan, and deposited in the Natural History Museum Vienna, Austria. Specimens of its sister species, Branchiobdella kozarovi Subchev, 1978, from its type area were examined and key morphological features were reported to justify naming the new species. The size, length and shape of the spermatheca and male reproductive organs in Branchiobdella turkestanensis are approximately half the size of those in B. kozarovi. Specific differences are the spermathecal bulb is ovoid, with a terete glandular atrium in B. turkestanensis, while B. kozarovi has a terete spermathecal bulb and ental process, and a curved, wide tubular glandular atrium.
New records of Cassidinae and Cerambycidae (Coleoptera: Chrysomeloidea) from Baturité Massif, Brazil, with the description of a new species of Charidotis (Coleoptera: Chrysomelidae: Cassidinae)
Authors: SIMÕES MARIANNA V. P. , SEKERKA LUKÁŠ , MOTA ADRIANA ANDRADE , NEARNS EUGENIO H. , MONNÉ MIGUEL A. , MONNÉ MARCELA L.
Published: July 2024

The Baturité massif, situated in Ceará state’s central hinterland, is one of the largest humid mountain ranges in the Brazilian semi-arid region. Flora and vertebrate surveys have been conducted in the region, revealing endemic species, as well as a combination of fauna and flora from neighboring biomes, the Atlantic and Amazon rainforests. However, invertebrate inventories have been scarce, leaving a significant portion of the region’s biodiversity unexplored. This paper presents the outcomes of collaborative expeditions conducted by the recently established Museu de História Natural do Ceará Prof. Dias da Rocha and Museu Nacional/Universidade do Rio de Janeiro to fill this knowledge gap by promoting surveys to document the region’s biodiversity. As a result, one new species of tortoise beetle is described from the massif, Charidotis rochai Simões and Sekerka, new species. Additionally, we report thirteen new state records for the beetle subfamilies Cassidinae (Chysomelidae), and Cerambycinae and Lamiinae (Cerambycidae). Among these records, eight genera are documented for the first time in the state of Ceará, and the species Centrocerum variatum (Newman, 1841) constitutes the first record in the northeastern region of Brazil. These findings underscore the significance of survey initiatives and the need for comprehensive biodiversity study within the Baturité massif.
Two-year survival after scheduled extubation in patients with pneumonia or ARDS: a prospective observational study
Authors: Chai Xuemin , Ma Mengyi , Hu Wenhui , Bai Linfu , Duan Jun
Published: July 2024

Purpose

To report two-year survival after scheduled extubation in patients with pneumonia or acute respiratory distress syndrome (ARDS).

Methods

This was a prospective observational study performed in a respiratory ICU of a teaching hospital. Pneumonia or ARDS patients who successfully completed a spontaneous breathing trial were enrolled. Data were collected before extubation. Patients were followed up to two years by phone every 3 months.

Results

A total of 230 patients were enrolled in final analysis. One-, 3-, 6-, 12-, and 24-month survival was 77.4%, 63.8%, 61.3%, 57.8%, and 47.8%, respectively. Cox regression shows that Charlson comorbidity index (hazard ratio: 1.20, 95% confidence interval: 1.10–1.32), APACHE II score before extubation (1.11, 1.05–1.17), cough peak flow before extubation (0.993, 0.986–0.999), and extubation failure (3.96, 2.51–6.24) were associated with two-year mortality. To predict death within two years, the area under the curve of receiver operating characteristic was 0.79 tested by Charlson comorbidity index, 0.75 tested by APACHE II score, and 0.75 tested by cough peak flow. Two-year survival was 31% and 77% in patients with Charlson comorbidity index ≥ 1 and < 1, 28% and 62% in patients with APACHE II score ≥ 12 and < 12, and 64% and 17% in patients with cough peak flow > 58 and ≤ 58 L/min, respectively.

Conclusions

Comorbidity, disease severity, weak cough and extubation failure were associated with increased two-year mortality in pneumonia or ARDS patients who experienced scheduled extubation. It provides objective information to caregivers to improve decision-making process during hospitalization and post discharge.
Stepping towards health: a scoping review of square-stepping exercise protocols and outcomes in older adults
Authors: Siqueira Vitor A. A. A. , Shigematsu Ryosuke , Sebastião Emerson
Published: July 2024

Background

Square-Stepping Exercise (SSE) is a type of physical-cognitive exercise. Such exercise has been used as an exercise approach in different studies with older adults. This scoping review provides an overview of the protocols and outcomes of studies employing the SSE in older adults.

Methods

We searched in the PubMed, CINAHL, Scopus, CABI Global Health, and Web of Science databases for articles published between 2006 (first research article published on SSE) to December 2023 that met a robust inclusion criterion. The search yielded 424 articles, and after inclusion criteria being applied, 37 articles were included in the final analysis.

Results

A total of 37 studies were included in the final analysis. Thirty-three out of the 37 studies focused on apparently healthy older adults, while four were conducted with older adults with neurological disease (i.e., multiple sclerosis, Parkinson’s disease, and stroke). Most studies (n = 25) adopted an experiment (i.e., randomized controlled trial) or quasi-experimental approach, while 12 were classified as non-randomized (i.e., cross-sectional, mixed methods). The studies were conducted in different parts of the globe and adopted three major formats of intervention delivery, namely in-person, online, and home-based. Frequency, SSE session duration and intervention length significantly varied among studies, and reported outcomes were in the domains of physical and cognitive function.

Conclusion

This review comprehensively described the characteristics of 37 studies employing SSE in apparently healthy older adults and older adults with neurological diseases. The findings demonstrated that SSE has been used by researchers across the globe, adopting a variety of forms of delivery, and to particularly improve physical and cognitive function of different segments of the older adult population. The review further identified important gaps in research, including the restricted outcomes, and the lack of studies combining SSE with more traditional exercise modalities to address potential combinatory effects.
Dietary diversity and iron deficiency anemia among a cohort of singleton pregnancies: a cross-sectional study
Authors: Al-Bayyari Nahla , Al Sabbah Haleama , Hailat Marah , AlDahoun Hadeel , Abu-Samra Haya
Published: July 2024

Background

Iron deficiency anemia (IDA) is considered one of the most common medical disorders observed during pregnancy. In low- and middle-income countries (LMICs), anemia and micronutrients deficiencies among pregnant women are associated with low consumption of animal products, monotonous starchy-diets, and seasonal consumption of vegetables and fruits.

Methods

A cross-sectional study was conducted with 198 pregnant mothers aged between 19–45 years who visited the antenatal care clinics in Northern Jordan to document the prevalence of IDA and to describe the associations between dietary diversity, diet quality scores and oral iron supplementation with the pregnant women iron status. Participants were stratified into three groups by gestational age (n = 66 women per group). Gestational age, blood parameters, minimum dietary diversity score (MDD-W), and prime diet quality score for healthy (PDQSHF) and unhealthy foods (PDQSUF) were assessed using 24- hour dietary recall.

Results

Prevalence of mild to moderate anemia was 27.8% among pregnant women. Third-trimester pregnant women were most affected. 52.5% have depleted iron stores (ferritin < 15 ng/ml), of them 30.8% have iron deficiency, and 21.7% have IDA. The (M ± SD) of the MDD-W, PDQSHF, and PDQSUF were 4.8 ± 1.6, 12.8 ± 3.9, and 7.2 ± 2.8 respectively. 52.5% achieved the MDD-W, 68% consumed < 4 servings/week of healthy food groups, and 50% consumed > 4 servings/week of unhealthy food groups. Mothers with higher MDD-W and PDQS had higher Hb and serum concentrations. Those taking iron supplements had significantly (p = 0.001) higher means of Hb, serum ferritin, and gestational weight gain. Significant differences were also found between PDQSHF, PDQSUF and the first and third trimester.

Conclusions

Mild to moderate IDA is prevalent among pregnant mothers, especially in the third trimester. However, the prevalence of IDA among Jordanian pregnant women is lower than the global average. A high-quality, diverse diet, combined with oral iron supplementation and food fortification with iron, will help improve iron status, prevent anemia, and reduce its prevalence.
Biomarkers of tissue remodelling are elevated in serum of COVID-19 patients who develop interstitial lung disease - an exploratory biomarker study
Authors: Breisnes Helene Wallem , Leeming Diana Julie , Karsdal Morten Asser , Burke Hannah , Freeman Anna , Wilkinson Tom , Fazleen Aishath , Bülow Sand Jannie Marie
Published: July 2024

Background

Coronavirus disease 2019 (COVID-19) is a viral pneumonia that can result in serious respiratory illness. It is associated with extensive systemic inflammation, changes to the lung extracellular matrix, and long-term lung impairment such as interstitial lung disease (ILD). In this study, the aim was to investigate whether tissue remodelling, wound healing, and neutrophil activity is altered in patients with COVID-19 and how these relate to the development of post-COVID ILD.

Method

Serum samples were collected from 63 patients three months after discharge as part of the Research Evaluation Alongside Clinical Treatment study in COVID-19 (REACT COVID-19), 10 of whom developed ILD, and 16 healthy controls. Samples were quantified using neo-epitope specific biomarkers reflecting tissue stiffness and formation (PC3X, PRO-C3, and PRO-C6), tissue degradation (C1M, C3M, and C6M), wound healing (PRO-FIB and X-FIB), and neutrophil activity (CPa9-HNE and ELP-3).

Results

Mean serum levels of PC3X (p < 0.0001), PRO-C3 (p = 0.002), C3M (p = 0.009), PRO-FIB (p < 0.0001), CPa9-HNE (p < 0.0001), and ELP-3 (p < 0.0001) were significantly elevated in patients with COVID-19 compared to healthy controls. Moreover, PC3X (p = 0.023) and PRO-C3 (p = 0.032) were significantly elevated in post-COVID ILD as compared to COVID-19.

Conclusion

Serological biomarkers reflecting type III collagen remodelling, clot formation, and neutrophil activity were significantly elevated in COVID-19 and type III collagen formation markers were further elevated in post-COVID ILD. The findings suggest an increased type III collagen remodelling in COVID-19 and warrants further investigations to assess the potential of tissue remodelling biomarkers as a tool to identify COVID-19 patients at high risk of developing ILD.
Blood pressure is associated with knee pain severity in middle-aged and elderly individuals with or at risks for osteoarthritis: data from the Osteoarthritis Initiative
Authors: Liu Yao , Du Guiying
Published: July 2024

Background

Hypertension is a common comorbidity of osteoarthritis (OA). Joint pain is the main clinical manifestation of OA. Knowledge about the relationship between hypertension and OA pain is limited. This study aimed to investigate whether blood pressure parameters are associated with knee pain severity in individuals with or at risks for OA.

Methods

Our sample consisted of 2598 subjects (60.7% female, aged 45–79 years) collected from the Osteoarthritis Initiative. Blood pressure parameters included blood pressure stage, systolic blood pressure (SBP), diastolic blood pressure (DBP) and pulse pressure (PP). Radiographic evaluation using Kellgren-Lawrence system and pain severity evaluation using Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Knee Injury and Osteoarthritis Outcome Score (KOOS), and Numeric Rating Scale (NRS) were performed for right knee. Linear regression was used to examine the relationship between blood pressure parameters and knee pain severity.

Results

For the overall sample, blood pressure stage, SBP, and PP were positively correlated with WOMAC and NRS pain scores when adjusting for age, sex, and body mass index (BMI) (p ≤ 0.024) and were inversely correlated with KOOS score (p ≤ 0.004). After further adjusting for all covariates, PP remained a positive correlation with WOMAC score (p = 0.037) while other associations between blood pressure parameters and pain scores did not reach the statistical significance. In female, higher blood pressure stage, SBP, and PP were significantly associated with increased WOMAC and NRS scores and decreased KOOS score after adjustments of age and BMI (p ≤ 0.018). When adjusting for all covariates, the correlations of PP with WOMAC, KOOS and NRS scores remained significant (p = 0.008–0.049). In male sample, SBP was positively correlated with WOMAC score when adjusting for age and BMI (p = 0.050), but other associations between blood pressure parameters and pain scores were not statistically significant. No significant correlation was observed in male when further adjusting for other covariates.

Conclusions

Increased PP is a risk factor for knee pain and mainly affects females, which suggested that controlling PP may be beneficial in preventing or reducing knee pain in females with or at risks for OA.
Knowledge and attitude of traumatic dental injuries in Mongolian schoolteachers
Authors: Narantsetseg Tsetsegkhen , Naran-Ochir Ochbayar , Ganbold Enkhtsatsral , Yunden Ganbaatar , Bayartsogt Batzorig , Badral Batbayar , Altannamar Munkhdul , Batbayar Enkh-Orchlon
Published: July 2024

Background

Traumatic dental injury (TDI) is a growing public health concern worldwide, and children and adolescents are commonly affected. Because TDI often occurs at school, the response of teachers to these injuries is crucial. However, teachers in various countries have been shown to lack knowledge of effective TDI first-aid response and need an intervention to improve their knowledge. The aim of the study presented here was to ascertain and analyze teachers’ knowledge of and attitude about TDI in Mongolia.

Materials and methods

A cross-sectional study of full-time teachers in Mongolia was performed using an online questionnaire (compiled from relevant studies) from September 2022 to December 2022. The questionnaire consists of 47 items and among them 14 were used to assess the teacher’s knowledge, and 5 were for attitude towards TDI. The maximum possible score was 14 points and grouped as follows good, moderate, and poor. T-test, ANOVA test (post-hoc) and linear regression analysis were performed.

Results

The online survey provided quantitative data from 2821 participants: 28% were elementary school teachers, 29% middle school teachers, and 42% high school teachers. Their mean age was 36.7 ± 9 years, and 2433 (86%) were female. The mean score of the TDI knowledge of the teachers was 5.3 ± 2.1 out of 14. The ANOVA test showed that older (p < 0.01) and more experienced teachers (p < 0.01) had higher scores. Teachers in the eastern (5.46 ± 2.2) and southern (5.49 ± 2.2) provinces had higher mean scores than in the other provinces (p < 0.02). Teachers majored in natural sciences (5.4 ± 2.2) had a higher score than those in the social sciences (5.2 ± 2.1) and difference were significant (p < 0.02). The multiple regression model statistically significantly predicted a one-year increase in experience, the TDI score increased 0.034 (95% CI 0.026, 0.043) unit.

Conclusions

The knowledge regarding TDI and effective first-aid response to such injury is low in teachers in Mongolia. However, the teachers’ attitude about TDI was positive and they wanted to learn and improve their knowledge. Therefore, further education and training programs are advised.
Antimalarial efficacy test of the aqueous crude leaf extract of Coriandrum sativum Linn.: an in vivo multiple model experimental study in mice infected with Plasmodium berghei
Authors: Habte Getu , Habte Sisay , Jilo Oda , Alemu Wondwosen , Eyasu Kedir , Meka Welela , Shifera Getabalew , Gezimu Wubishet , Dugasa Milkias , Tamiru Sanbato , Mamo Meta , Kelecha Abiyot
Published: July 2024

Background

Malaria continues to wreak havoc on the well-being of the community. Resistant parasites are jeopardizing the treatment. This is a wake-up call for better medications. Folk plants are the key starting point for antimalarial drug discovery. After crushing and mixing the leaves of Coriandrum sativum with water, one cup of tea is drunk daily for a duration of three to five days as a remedy for malaria by local folks in Ethiopia. Additionally, in vitro experiments conducted on the plant leaf extract elsewhere have also demonstrated the plant’s malaria parasite inhibitory effect. There has been no pharmacologic research to assert this endowment in animals, though. This experiment was aimed at evaluating the antimalarial efficacy of C. sativum in Plasmodium berghei infected mice.

Methods

The plant's leaf was extracted using maceration with distilled water. The extract was examined for potential acute toxicity. An evaluation of secondary phytoconstituents was done. Standard antimalarial screening models (prophylactic, chemosuppressive, curative tests) were utilized to assess the antiplasmodial effect. In each test, thirty mice were organized into groups of five. To the three categories, the test substance was given at doses of 100, 200 and 400 mg/kg/day before or after the commencement of P. berghei infection. Positive and negative control mice were provided Chloroquine and distilled water, respectively. Rectal temperature, parasitemia, body weight, survival time and packed cell volume were ultimately assessed. Analysis of the data was performed using Statistical Package for Social Sciences.

Results

No toxicity was manifested in mice. The extract demonstrated a significant inhibition of parasitemia (p < 0.05) in all the models. The inhibition of parasite load was highest with the upper dose in the suppressive test (82.74%) followed by the curative procedure (78.49%). Likewise, inhibition of hypothermia, weight loss hampering, improved survival and protection against hemolysis were elicited by the extract.

Conclusions

The results of our experimental study revealed that the aqueous crude leaf extract of C. sativum exhibits significant antimalarial efficacy in multiple in vivo models involving mice infected with P. berghei. Given this promising therapeutic attribute, in depth investigation on the plant is recommended.
Antimicrobial use, residues and resistance in fish production in Africa: systematic review and meta-analysis
Authors: Moffo Frédéric , Ndebé Mohamed Moustapha Fokom , Tangu Mildred Naku , Noumedem Ranyl Nguena Guefack , Awah-Ndukum Julius , Mouiche Mohamed Moctar Mouliom
Published: July 2024

In low- and middle-income countries, data on antimicrobial use (AMU) and antimicrobial resistance (AMR) in aquaculture are scarce. Therefore, summarizing documented data on AMU, antimicrobial residue (AR), and AMR in aquaculture in Africa is key to understanding the risk to public health. Google Scholar, PubMed, African Journals online, and Medline were searched for articles published in English and French following the PRISMA guidelines. A structured search string was used with strict inclusion and exclusion criteria to retrieve and screen the articles. The pooled prevalence and 95% confidence intervals were calculated for each pathogen–antimicrobial pair using random effects models. Among the 113 full-text articles reviewed, 41 met the eligibility criteria. The majority of the articles reported AMR (35; 85.4%), while a few were on AMU (3; 7.3%) and AR (3; 7.3%) in fish. The articles originated from West Africa (23; 56.1%), North Africa (8; 19.7%), and East Africa (7; 17.1%). Concerning the antimicrobial agents used in fish farming, tetracycline was the most common antimicrobial class used, which justified the high prevalence of residues (up to 56.7%) observed in fish. For AMR, a total of 69 antimicrobial agents were tested against 24 types of bacteria isolated. Bacteria were resistant to all classes of antimicrobial agents and exhibited high levels of multidrug resistance. Escherichia coli, Salmonella spp., and Staphylococcus spp. were reported in 16, 10, and 8 studies, respectively, with multidrug resistance rates of 43.1% [95% CI (32.0–55.0)], 40.3% [95% CI (24.1–58.1)] and 31.3% [95% CI (17.5–49.4)], respectively. This review highlights the high multidrug resistance rate of bacteria from aquaculture to commonly used antimicrobial agents, such as tetracycline, ampicillin, cotrimoxazole, gentamicin, and amoxicillin, in Africa. These findings also highlighted the lack of data on AMU and residue in the aquaculture sector, and additional efforts should be made to fill these gaps and mitigate the burden of AMR on public health in Africa.
Left anterior mini-thoracotomy: an alternative approach for pulmonary valve replacement after surgically corrected tetralogy of fallot
Authors: Ho Yan Le , Khamis Abu Yamin , Abdul Kareem Basheer Ahamed
Published: July 2024

Introduction

Pulmonary regurgitation (PR) remains a common sequela in patients following surgically corrected TOF, and may lead to progressive right ventricle dilatation and dysfunction. The conventional approach of redo-sternotomy for pulmonary valve replacement (PVR) is associated with increased operative time as well as risks of bleeding and injury to the heart and great vessels. Thus, left anterior mini-thoracotomy has become an alternative approach in eliminating the risks of redo-sternotomy in these patients. This series aimed to determine the outcomes of minimally invasive pulmonary valve replacement after surgical TOF correction.

Methods

A retrospective analysis was conducted on 24 patients with severe PR post-surgical TOF correction who underwent left anterior mini-thoracotomy PVR in Penang General Hospital from January 2021 to January 2023.

Results

The median age was 23.5 years (I.Q.range 17.6-36.3), with a male:female ratio of 1:4. Majority of patients had mild to moderate symptoms prior to surgery and 19 patients (79.1%) were on regular diuretics medication. All patients had severe free-flow PR with evidence of right ventricular dilatation and dysfunction. Magnetic Resonance Imaging and computed tomography of pulmonary artery were performed prior to surgery. Minimally invasive PVR was performed on all patients via left upper anterior mini-thoracotomy and femoral-femoral bypass without cardioplegic arrest. The operative time and cardiopulmonary bypass time were 208 (I.Q.range 172-324) and 98.6 minutes(I.Q.range 87.4-152.4) respectively. The time to wean off inotropes postoperatively was 6.2 hours (I.Q.range1.4-14.8), and no postoperative arrhythmia and chest re-exploration were reported. Most patients stayed in Intensive Care Unit (ICU) for 10.8 hours (I.Q.range 8.4-36.5), and the total hospital stay was 4.2 days (I.Q.range 3.4-7.6). 2 patients (11.1%) required blood transfusion postoperative. There was no paravalvular leak and no mortality during the follow-up period of up to 28 months.

Conclusion

Minimally invasive PVR after surgical correction of TOF is a safe alternative to the conventional redo-sternotomy approach in patients with favorable anatomy. This approach is able to reduce the risks associated with redo-sternotomy, particularly bleeding and injury to mediastinal structures, with the additional benefit of expedited recovery and hospital discharge. Our series has shown a safe and efficient approach in these patients with favorable outcomes.
The interplay of inflammation and remyelination: rethinking MS treatment with a focus on oligodendrocyte progenitor cells
Authors: Zveik Omri , Rechtman Ariel , Ganz Tal , Vaknin-Dembinsky Adi
Published: July 2024

Background

Multiple sclerosis (MS) therapeutic goals have traditionally been dichotomized into two distinct avenues: immune-modulatory-centric interventions and pro-regenerative strategies. Oligodendrocyte progenitor cells (OPCs) were regarded for many years solely in concern to their potential to generate oligodendrocytes and myelin in the central nervous system (CNS). However, accumulating data elucidate the multifaceted roles of OPCs, including their immunomodulatory functions, positioning them as cardinal constituents of the CNS’s immune landscape.

Main body

In this review, we will discuss how the two therapeutic approaches converge. We present a model by which (1) an inflammation is required for the appropriate pro-myelinating immune function of OPCs in the chronically inflamed CNS, and (2) the immune function of OPCs is crucial for their ability to differentiate and promote remyelination. This model highlights the reciprocal interactions between OPCs’ pro-myelinating and immune-modulating functions. Additionally, we review the specific effects of anti- and pro-inflammatory interventions on OPCs, suggesting that immunosuppression adversely affects OPCs’ differentiation and immune functions.

Conclusion

We suggest a multi-systemic therapeutic approach, which necessitates not a unidimensional focus but a harmonious balance between OPCs’ pro-myelinating and immune-modulatory functions.
Development and preliminary evaluation of a suicidal risk assessment protocol in a randomised controlled trial using the Patient Health Questionnaire (PHQ-9)
Authors: Wileman Vari , McGuinness Serena , Sweeney Louise , Norton Christine , Miller Laura , Stagg Imogen , O’Carroll Ronan , Moss-Morris Rona
Published: July 2024

Background

Participants in research trials often disclose severe depression symptoms, including thoughts of self-harm and suicidal ideation, in validated self-administered questionnaires such as the Patient Health Questionnaire (PHQ-9). However, there is no standard protocol for responding to such disclosure, and the opportunity to support people at risk is potentially missed. We developed and evaluated a risk assessment protocol for the IBD-BOOST randomised controlled trial (ISRCTN71618461 09/09/2019).

Methods

Participants completed the PHQ-9 at baseline and 6-month and 12-month follow-ups. The trial database automatically alerted the research team to risk assess participants. Trial researchers, trained in the protocol, contacted participants by telephone, completed the risk assessment, and signposted participants to appropriate professional services.

Results

Seven hundred eighty participants were randomised in the trial; 41 required risk assessment. One participant declined assessment, so 40 risk assessments were completed. Twenty-four participants were assessed as low-risk and 16 participants as medium-risk, with 12 declaring previous suicide attempts. None were rated as high-risk. Trial participants expressed appreciation for being contacted, and all except two wished to receive information about professional support services. Trial risk assessors reported positive experiences of conducting the risk assessment with suggestions for improvement, which resulted in minor modifications to the protocol.

Discussion

Our evaluation demonstrated that it was viable for a research trial team to successfully conduct a risk-assessment protocol for trial participants reporting thoughts of self-harm, with training and support from senior colleagues. Resources are required for training and delivery, but it is not unduly onerous. Trial participants appeared to find completing the assessment acceptable.
The effectiveness of expressive art therapy on infertile women undergoing surgery: study protocol for a randomized controlled trial
Authors: Liu Li , Yang Huiyan , Lei Anjiang , You Huaxuan
Published: July 2024

Background

Infertility produces infertility-related stress in both members of infertile couples, especially for infertile women. Some studies verified the negative relationship between infertility-related stress and outcomes of infertility treatments. Effective mental health care during fertility treatment is urgently needed, but there has been a lack of efficient support services. To reduce the infertility-related stress of infertile women, expressive art therapeutic schemes will be organized and implemented by certified international expressive art therapists.

Methods

This study is a randomized controlled trial. Participants in the intervention group will receive expressive art therapies after the baseline investigation. Expressive art therapies will be led by the certified international expressive art therapist. The interventions include progressive muscle relaxation training, music meditation and drawing therapy. Participants in the control group will receive routine care. The Hospital Anxiety and Depression Scale (HADS) and Fertility Problem Inventory (FPI) will be used to investigate the anxiety, depression, and infertility-related stress of all participants at admission and at discharge.

Discussion

This study will verify the effectiveness and efficiency of expressive art therapies for infertile women. The results will provide new knowledge on mental health care strategies for infertile women.

Trial registration

ChiCTR, ChiCTR2300070618. Registered 14 April 2023.
New insights into the early morphological evolution of sea turtles by re-investigation of Nichollsemys baieri, a three-dimensionally preserved fossil stem chelonioid from the Campanian of Alberta, Canada
Authors: Menon Juliette C. L. , Brinkman Donald B. , Hermanson Guilherme , Joyce Walter G. , Evers Serjoscha W.
Published: July 2024

The early evolution of Pan-Chelonioidea (sea turtles) is poorly understood. This is in part due to the rarity of undeformed skulls of definitive early stem chelonioids. In this work, we redescribe the holotype of Nichollsemys baieri using µCT scans and segmentations of the skull. This fossil is the best 3D preserved skull of any Campanian sea turtle, and includes partial “soft tissue” preservation. Nichollsemys is morphologically similar but clearly distinct from Toxochelys spp., and both show a mosaic of plesiomorphic and derived chelonioid features. The internal cranial anatomy documents the presence of derived characters in Nichollsemys baieri that are absent in Toxochelys spp., such as the loss of the epipterygoids and the rod-like shape of the rostrum basisphenoidale. Among the numerous plesiomorphic characters is the presence of a splenial bone, which was unnoticed before. An updated phylogenetic analysis retrieves Nichollsemys baieri as a non-protostegid early stem chelonioid in a slightly more crownward position than Toxochelys latiremis. Our phylogeny includes macrobaenids and protostegids as pan-chelonioids, and we find unorthodox results for dermochelyids. Thus, although Nichollsemys baieri provides important new insights into the early morphological evolution of sea turtles, much work remains to be done. As a completely 3D preserved specimen, we included Nichollsemys baieri into a recent landmark-based skull shape dataset of turtles. Morphospace analysis reveals an intermediate position between cryptodires and crown chelonioids. Based on these data, we also predict that Nichollsemys baieri was still capable of neck retraction, constraining the loss of this trait to more crownward pan-chelonioids.
Stakeholders’ participation in decreasing wildfire risk in the context of natural resource management in the Podpoľanie region of Slovakia
Authors: Brodrechtova Yvonne , Majlingová Andrea , Sedmák Róbert
Published: July 2024

Key Message

As the number and extent of wildfires are increasing due to climate change and human impacts, the demand for effective risk reduction strategies is growing. Due to limited resources or management capabilities in Slovakia, appropriate multistakeholder participation could help decrease the risk of wildfires while continuing to implement the principles of sustainable forest management. Thus, we recommend improving relationships with responsible stakeholders and increasing their knowledge.

Context

Although the frequency of wildfires has declined in Slovakia, the total burned area per year and the average burned area per fire have increased rapidly, mainly due to traditional land management and increasing numbers of natural disasters resulting from climate change.

Aim

The objective of this study is to assess the participation and management of stakeholders in the region of Podpoľanie, which is the region of Slovakia that is most prone to wildfires. In particular, this study investigates the questions of who is accountable, in what networks, and with regard to what issues with the goal of enhancing efforts to combat wildfires.

Methods

This single-country case study features an exploratory sequential mixed-methods design. While stakeholders’ participation was explored via face-to-face interviews and interest-influence matrices, stakeholders’ prioritization was assessed via an online survey and the quantitative mapping of stakeholders’ involvement, power, interest, and knowledge.

Results

We separately identified primary stakeholders (e.g., fire departments and state-owned forest enterprises) and secondary stakeholders (e.g., municipalities and community members). Tasks related to efforts to combat wildfires were largely in compliance with legal regulations and other mandates. Nonetheless, some stakeholders (e.g., governmental organizations involved in nature protection or nonstate forest owners and the associated enterprises) lacked the knowledge, experience, or responsibility necessary to perform these tasks.

Conclusion

These stakeholders should be informed and monitored closely to achieve outcomes that can benefit a variety of stakeholders. We recommend a plan that involves improving the relationships among primary and secondary stakeholders, increasing their knowledge, and promoting effective participation to decrease the risk of wildfires in the region.
Understanding the impact of COVID-19 on antibiotic use in Canadian primary care: a matched-cohort study using EMR data
Authors: Morkem Rachael , Smith Glenys , Knight Braden , Wong Sabrina T. , Barber David
Published: July 2024

Background

Inappropriate or overuse of antibiotic prescribing in primary care highlights an opportunity for antimicrobial stewardship (AMS) programs aimed at reducing unnecessary use of antimicrobials through education, policies and practice audits that optimize antibiotic prescribing. Evidence from the early part of the pandemic indicates a high rate of prescribing of antibiotics for patients with COVID-19. It is crucial to surveil antibiotic prescribing by primary care providers from the start of the pandemic and into its endemic stage to understand the effects of the pandemic and better target effective AMS programs.

Methods

This was a matched pair population-based cohort study that used electronic medical record (EMR) data from the Canadian Primary Care Sentinel Surveillance Network (CPCSSN). Participants included all patients that visited their primary care provider and met the inclusion criteria for COVID-19, respiratory tract infection (RTI), or non-respiratory or influenza-like-illness (negative). Four outcomes were evaluated (a) receipt of an antibiotic prescription; (b) receipt of a non-antibiotic prescription; (c) a subsequent primary care visit (for any reason); and (d) a subsequent primary care visit with a bacterial infection diagnosis. Conditional logistic regression was used to evaluate the association between COVID-19 and each of the four outcomes. Each model was adjusted for location (rural or urban), material and social deprivation, smoking status, alcohol use, obesity, pregnancy, HIV, cancer and number of chronic conditions.

Results

The odds of a COVID-19 patient receiving an antibiotic within 30 days of their visit is much lower than for patients visiting for RTI or for a non-respiratory or influenza-like-illnesses (AOR = 0.08, 95% CI[0.07, 0.09] compared to RTI, and AOR = 0.43, 95% CI[0.38, 0.48] compared to negatives). It was found that a patient visit for COVID-19 was much less likely to have a subsequent visit for a bacterial infection at all time points.

Conclusions

Encouragingly, COVID-19 patients were much less likely to receive an antibiotic prescription than patients with an RTI. However, this highlights an opportunity to leverage the education and attitude change brought about by the public health messaging during the COVID-19 pandemic (that antibiotics cannot treat a viral infection), to reduce the prescribing of antibiotics for other viral RTIs and improve antibiotic stewardship.
Evaluating local plant species for effective fall armyworm management strategies in Taiwan
Authors: Tsai Yuan-Ching , Luo Pei-Qi , Sung Chang-Lin , Li Yi , Hu Fang-Yu , Wang Chih-Lu , Chen Yi-Ning , Hsu Ju-Hsin , Liao Chien-En , Chang Shyh-Rong , Chuang Wen-Po
Published: July 2024

Background

The emergence of Spodoptera frugiperda (fall armyworm; FAW) in the world has raised concerns regarding its impact on crop production, particularly on corn and sorghum. While chemical control and Bt crops have been effective in managing FAW damage, the development of pesticide-resistant and Bt-resistant strains necessitates alternative control methods. The push-pull farming system has gained attention, but direct utilization of African plant species in Taiwan faces challenges due to invasive potential and climatic disparities. Therefore, identifying and evaluating suitable local plant species, such as Napier grass (Pennisetum purpureum), Desmodium species, and signal grass (Brachiaria brizantha), is crucial for implementing effective FAW management strategies in Taiwan.

Results

In screening fifty Napier grass germplasms, all demonstrated an antibiotic effect, reducing leaf consumption compared to corn. Notably, thirty-five germplasms exhibited robust antibiotic traits, decreasing FAW consumption and increasing mortality rates. Three Napier grass germplasms also attracted more female moths for oviposition. Further evaluation of selected Napier grass germplasms and signal grass demonstrated efficacy in reducing FAW larval weight and survival duration. Additionally, Desmodium species, particularly D. uncinatum, showed promising toxicity against FAW larvae.

Conclusion

Our findings support the effectiveness of selected Napier grass germplasms and signal grass as pull plants, and highlight the potential of D. uncinatum as a push plant in FAW management strategies in Taiwan.
Fine-scale characterization of the soybean rhizosphere microbiome via synthetic long reads and avidity sequencing
Authors: Hale Brett , Watts Caitlin , Conatser Matthew , Brown Edward , Wijeratne Asela J.
Published: July 2024

Background

The rhizosphere microbiome displays structural and functional dynamism driven by plant, microbial, and environmental factors. While such plasticity is a well-evidenced determinant of host health, individual and community-level microbial activity within the rhizosphere remain poorly understood, due in part to the insufficient taxonomic resolution achieved through traditional marker gene amplicon sequencing. This limitation necessitates more advanced approaches (e.g., long-read sequencing) to derive ecological inferences with practical application. To this end, the present study coupled synthetic long-read technology with avidity sequencing to investigate eukaryotic and prokaryotic microbiome dynamics within the soybean (Glycine max) rhizosphere under field conditions.

Results

Synthetic long-read sequencing permitted de novo reconstruction of the entire 18S-ITS1-ITS2 region of the eukaryotic rRNA operon as well as all nine hypervariable regions of the 16S rRNA gene. All full-length, mapped eukaryotic amplicon sequence variants displayed genus-level classification, and 44.77% achieved species-level classification. The resultant eukaryotic microbiome encompassed five kingdoms (19 genera) of protists in addition to fungi – a depth unattainable with conventional short-read methods. In the prokaryotic fraction, every full-length, mapped amplicon sequence variant was resolved at the species level, and 23.13% at the strain level. Thirteen species of Bradyrhizobium were thereby distinguished in the prokaryotic microbiome, with strain-level identification of the two Bradyrhizobium species most reported to nodulate soybean. Moreover, the applied methodology delineated structural and compositional dynamism in response to experimental parameters (i.e., growth stage, cultivar, and biostimulant application), unveiled a saprotroph-rich core microbiome, provided empirical evidence for host selection of mutualistic taxa, and identified key microbial co-occurrence network members likely associated with edaphic and agronomic properties.

Conclusions

This study is the first to combine synthetic long-read technology and avidity sequencing to profile both eukaryotic and prokaryotic fractions of a plant-associated microbiome. Findings herein provide an unparalleled taxonomic resolution of the soybean rhizosphere microbiota and represent significant biological and technological advancements in crop microbiome research.
Proteomic insights into extracellular vesicles in ALS for therapeutic potential of Ropinirole and biomarker discovery
Authors: Kato Chris , Ueda Koji , Morimoto Satoru , Takahashi Shinichi , Nakamura Shiho , Ozawa Fumiko , Ito Daisuke , Daté Yugaku , Okada Kensuke , Kobayashi Naoki , Nakahara Jin , Okano Hideyuki
Published: July 2024

Background

Extracellular vesicles (EVs) hold the potential for elucidating the pathogenesis of amyotrophic lateral sclerosis (ALS) and serve as biomarkers. Notably, the comparative and longitudinal alterations in the protein profiles of EVs in serum (sEVs) and cerebrospinal fluid (CSF; cEVs) of sporadic ALS (SALS) patients remain uncharted. Ropinirole hydrochloride (ROPI; dopamine D2 receptor [D2R] agonist), a new anti-ALS drug candidate identified through induced pluripotent stem cell (iPSC)-based drug discovery, has been suggested to inhibit ALS disease progression in the Ropinirole Hydrochloride Remedy for Amyotrophic Lateral Sclerosis (ROPALS) trial, but its mechanism of action is not well understood. Therefore, we tried to reveal longitudinal changes with disease progression and the effects of ROPI on protein profiles of EVs.

Methods

We collected serum and CSF at fixed intervals from ten controls and from 20 SALS patients participating in the ROPALS trial. Comprehensive proteomic analysis of EVs, extracted from these samples, was conducted using liquid chromatography/mass spectrometer (LC/MS). Furthermore, we generated iPSC-derived astrocytes (iPasts) and performed RNA sequencing on astrocytes with or without ROPI treatment.

Results

The findings revealed notable disparities yet high congruity in sEVs and cEVs protein profiles concerning disease status, time and ROPI administration. In SALS, both sEVs and cEVs presented elevated levels of inflammation-related proteins but reduced levels associated with unfolded protein response (UPR). These results mirrored the longitudinal changes after disease onset and correlated with the revised ALS Functional Rating Scale (ALSFRS-R) at sampling time, suggesting a link to the onset and progression of SALS. ROPI appeared to counteract these changes, attenuating inflammation-related protein levels and boosting those tied to UPR in SALS, proposing an anti-ALS impact on EV protein profiles. Reverse translational research using iPasts indicated that these changes may partly reflect the DRD2-dependent neuroinflammatory inhibitory effects of ROPI. We have also identified biomarkers that predict diagnosis and disease progression by machine learning-driven biomarker search.

Conclusions

Despite the limited sample size, this study pioneers in reporting time-series proteomic alterations in serum and CSF EVs from SALS patients, offering comprehensive insights into SALS pathogenesis, ROPI-induced changes, and potential prognostic and diagnostic biomarkers.