bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the Impact of First-Life Lithium-Ion Battery Degradation on Second-Life Performance
Authors: Tasnim Mowri Sadia , Barai Anup , Moharana Sanghamitra , Gupta Aniruddha , Marco James
Published: January 2024

The driving and charging behaviours of Electric Vehicle (EV) users exhibit considerable variation, which substantially impacts the battery degradation rate and its root causes. EV battery packs undergo second-life application after first-life retirement, with SoH measurements taken before redeployment. However, the impact of the root cause of degradation on second-life performance remains unknown. Hence, the question remains whether it is necessary to have more than a simple measure of state of health (SoH) before redeployment. This article presents experimental data to investigate this. As part of the experiment, a group of cells at around 80% SoH, representing retired EV batteries, were cycled using a representative second-life duty cycle. Cells with a similar root cause of degradation in the first life (100–80% SoH) exhibited the same degradation rate in second life after being cycled with the same duty cycle during the second life. When the root cause of degradation in the first life is different, the degradation rate in the second life may not be the same. These findings suggest that the root cause of a cell’s first-life degradation impacts how it degrades in its second life. Postmortem analysis (photographic and SEM images) reveals the similar physical condition of negative electrodes which have similar degradation rates in their second life cycle. This demonstrates that cells with a similar first life SoH and root cause of degradation indeed experience a similar life during their second life. The experimental results, along with the subsequent postmortem analysis, suggest that relying solely on SoH assessment is insufficient. It is crucial to take into account the root causes of cell degradation before redeployment.
The Effect of Using a Geopedological Approach in Determining Land Quality Indicators, Land Degradation, and Development (Case Study: Caspian Sea Coast)
Authors: Samiei-Fard Ramin , Heidari Ahmad , Drohan Patrick J. , Mahmoodi Shahla , Ghatrehsamani Shirin
Published: January 2024

This study addresses the escalating global concern surrounding land degradation (LD) and its far-reaching implications on water and nutrient availability, as well as on human health and well-being. Focused on the southeastern Caspian Sea region, this research employs a novel remote sensing geo-pedological methodology to comprehensively assess soil and land quality dynamics, particularly influenced by salts, and investigates the intricate relationship between LD and soil development. The study area, marked by a susceptibility to seawater level fluctuations and diverse landforms (lagoons, barriers, and coastal plains) offers a unique opportunity for geopedologic analysis. Utilizing particle size distribution data, six distinct landforms are identified, providing insights into the region’s complex sedimentary history. A soil quality assessment is conducted remotely through the calculation of two indexes—the Integrated Quality Index (IQI) and the Nemoro Quality Index (NQI)—employing both Total Data Set (TDS) and Minimum Data Set (MDS) methodologies. The investigation highlights the role of soluble salts in shaping soil quality, thereby influencing LD and development dynamics. The differentiation of landforms significantly enhances classification accuracy, providing a more nuanced understanding of the multifaceted factors governing LD. The study’s implications extend beyond the southeastern Caspian Sea region, and demonstrate that the potential for incorporating a geopedologic approach when assessing soil and land quality dynamics in arid regions globally. Our analytic approach can inform policymakers and land managers when making decisions to combat LD and foster sustainable land development. This research also contributes towards advancing knowledge in geopedology by providing a robust foundation for future studies aimed at enhancing land management practices in the face of ongoing environmental challenges.
Fine-Tuning the Expression of the Glycolate Biosynthetic Pathway in Escherichia coli Using Synthetic Promoters
Authors: Zhao Mei , Shang Jie , Chen Jiaojiao , Zabed Hossain M. , Qi Xianghui
Published: January 2024

Glycolate plays an important role as a platform chemical in both polymeric material and cosmetic industries. However, the microbial production of glycolate often encounters challenges associated with unbalanced metabolic flux, leading to a notably low titer. Additionally, the use of expensive inducers, such as IPTG, contributes to an increase in the overall production cost. To address these issues, the key enzymes involved in the glycolate biosynthetic route, including citrate synthase (gltA), isocitrate lyase (aceA), isocitrate dehydrogenase kinase/phosphatase (aceK) and glyoxylate reductase (ycdW), were overexpressed in E. coli under the control of inducible promoters with varying strengths in order to determine the optimal combination. Subsequently, the glycolate pathway was further modulated by replacing inducible promoters with various constitutive synthetic promoters. Through this systematic optimization, the best strain, named Mgly4T1562, produced 3.02 g/L glycolate with 97.32% theoretical yield in shake-flask cultivation. The titer further increased to 15.53 g/L in a fed-batch experiment. Notably, this study marks the first successful utilization of synthetic promoters in tuning the glycolate biosynthetic pathway for glycolate biosynthesis. The strategy presented in this research holds significant promise for facilitating the cost-effective and industrially viable production of glycolate without the need for expensive inducers.
A Holistic Review of Machine Learning Adversarial Attacks in IoT Networks
Authors: Khazane Hassan , Ridouani Mohammed , Salahdine Fatima , Kaabouch Naima
Published: January 2024

With the rapid advancements and notable achievements across various application domains, Machine Learning (ML) has become a vital element within the Internet of Things (IoT) ecosystem. Among these use cases is IoT security, where numerous systems are deployed to identify or thwart attacks, including intrusion detection systems (IDSs), malware detection systems (MDSs), and device identification systems (DISs). Machine Learning-based (ML-based) IoT security systems can fulfill several security objectives, including detecting attacks, authenticating users before they gain access to the system, and categorizing suspicious activities. Nevertheless, ML faces numerous challenges, such as those resulting from the emergence of adversarial attacks crafted to mislead classifiers. This paper provides a comprehensive review of the body of knowledge about adversarial attacks and defense mechanisms, with a particular focus on three prominent IoT security systems: IDSs, MDSs, and DISs. The paper starts by establishing a taxonomy of adversarial attacks within the context of IoT. Then, various methodologies employed in the generation of adversarial attacks are described and classified within a two-dimensional framework. Additionally, we describe existing countermeasures for enhancing IoT security against adversarial attacks. Finally, we explore the most recent literature on the vulnerability of three ML-based IoT security systems to adversarial attacks.
The Role of Particle Inertia and Thermal Inertia in Heat Transfer in a Non-Isothermal Particle-Laden Turbulent Flow
Authors: Zandi Pour Hamid Reza , Iovieno Michele
Published: January 2024

We present an analysis of the effect of particle inertia and thermal inertia on the heat transfer in a turbulent shearless flow, where an inhomogeneous passive temperature field is advected along with inertial point particles by a homogeneous isotropic velocity field. Eulerian–Lagrangian direct numerical simulations are carried out in both one- and two-way coupling regimes and analyzed through single-point statistics. The role of particle inertia and thermal inertia is discussed by introducing a new decomposition of particle second-order moments in terms of correlations involving Lagrangian acceleration and time derivative of particles. We present how particle relaxation times mediate the level of particle velocity–temperature correlation, which gives particle contribution to the overall heat transfer. For each thermal Stokes number, a critical Stokes number is individuated. The effect of particle feedback on the attenuation or enhancement of fluid temperature variance is presented. We show that particle feedback enhances fluid temperature variance for Stokes numbers less than one and damps is for larger than one Stokes number, regardless of the thermal Stokes number, even if this effect is amplified by an increasing thermal inertia.
Assessing the Role of Gaseous Chlorine Dioxide in Modulating the Postharvest Ripening of Keitt Mangoes through the Induction of Ethylene Biosynthesis
Authors: Zhang Dongwei , Liu Binxiong , Wu Shaoyi , Li Changcheng , Fang Ting , Tian Meiling
Published: January 2024

Consumer acceptance of Keitt mangoes (Mangifera indica L.) is significantly affected by their slow postharvest ripening. This work used gaseous chlorine dioxide (ClO2(g)) to prepare the ready-to-eat Keitt mango and explored the potential mechanisms for the mango ripening. Harvested mangoes were treated with 20 mg·L−1 of ClO2(g) or ethephon for 3 h (25 °C) and left in a climatic chamber with a temperature of 25 ± 1 °C and a relative humidity of 85 ± 5% for 4 d. The results showed that ClO2(g) treatment significantly promoted the orange coloration of mango flesh compared to the untreated control group. Moreover, ClO2(g) treatment significantly elevated the total soluble solids, total soluble sugar, and total carotenoids content of mangoes, whereas the firmness and titratable acidity were reduced. ClO2(g)-treated mangoes reached the edible window on day 2, as did mangoes treated with ethephon at the same concentration, except that the sweetness was prominent. The residual ClO2 level of the mangoes was <0.3 mg/kg during the whole storage time, which is a safe level for fruit. In addition, ClO2(g) significantly advanced the onset of ethylene peaks by 0.5 days and increased its production between days 0.5 and 2 compared to the control group. Consistently, the genes involved in ethylene biosynthesis including miACS6, miACO1, and miACO were upregulated. In sum, ClO2(g) can be a potential technique to reduce the time for harvested mango to reach the edible window, and it functions in modulating postharvest ripening by inducing ethylene biosynthesis.
Quantitative Determination of Aflatoxin B1 in Maize and Feed by ELISA and Time-Resolved Fluorescent Immunoassay Based on Monoclonal Antibodies
Authors: Han Shiyun , Yang Yalin , Chen Ting , Yang Bijia , Ding Mingyue , Wen Hao , Xiao Jiaxu , Cheng Guyue , Tao Yanfei , Hao Haihong , Peng Dapeng
Published: January 2024

In this study, a highly sensitive monoclonal antibody (mAb) was developed for the detection of aflatoxin B1 (AFB1) in maize and feed. Additionally, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluorescence immunoassay assay (TRFICA) were established. Firstly, the hapten AFB1-CMO was synthesized and conjugated with carrier proteins to prepare the immunogen for mouse immunization. Subsequently, mAb was generated using the classical hybridoma technique. The lowest half-maximal inhibitory concentration (IC50) of ic-ELISA was 38.6 ng/kg with a linear range of 6.25–100 ng/kg. The limits of detections (LODs) were 6.58 ng/kg and 5.54 ng/kg in maize and feed, respectively, with the recoveries ranging from 72% to 94%. The TRFICA was developed with a significantly reduced detection time of only 21 min, from sample processing to reading. Additionally, the limits of detection (LODs) for maize and feed were determined to be 62.7 ng/kg and 121 ng/kg, respectively. The linear ranges were 100–4000 ng/kg, with the recoveries ranging from 90% to 98%. In conclusion, the development of AFB1 mAb and the establishment of ic-ELISA for high-throughput sample detection, as well as TRFICA for rapid detection presented robust tools for versatile AFB1 detection in different scenarios.
Formulation of Antioxidant Gummies Based on Gelatin Enriched with Citrus Fruit Peels Extract
Authors: Aiello Francesca , Caputo Paolino , Oliviero Rossi Cesare , Restuccia Donatella , Spizzirri Umile Gianfranco
Published: January 2024

In this work, the peels of red and blonde oranges as well as lemons were efficiently (5.75–9.65% yield) extracted by hydroalcoholic solution with ultrasound assistance and employed as active molecule sources in the preparation of functional gummies. Antioxidant performances of the hydroalcoholic extracts were characterized by colorimetric assays, whereas LC–HRMS analyses identified the main bioactive compounds (phenolic acids and flavonoids). The highest scavenging activity was recorded for lemon extract in an aqueous environment (IC50 = 0.081 mg mL−1). An ecofriendly grafting procedure was performed to anchor polyphenols to gelatin chains, providing macromolecular systems characterized by thermal analysis and antioxidant properties. Scavenger abilities (IC50 = 0.201–0.454 mg mL−1) allowed the employment of the conjugates as functional ingredients in the preparation of gummies with remarkable antioxidant and rheological properties over time (14 days). These findings confirmed the possible employment of highly polluting wastes as valuable sources of bioactive compounds for functional gummies preparation.
Effect of Whole Egg Liquid on Physicochemical, Quality, Fermentation and Sensory Characteristics of Yogurt
Authors: Yang Lijie , Wang Lifeng , Chi Yujie , Chi Yuan
Published: January 2024

With the purpose of developing an alternative set yogurt with high consumer acceptability, liquid whole egg (LWE), at levels that varied from 0 to 30%, was incorporated into set yogurt, and the effects on the physicochemical, quality, fermentation, and sensory characteristics of yogurt were evaluated. The fat content was lower in egg yogurt than in control yogurt. All color variables were significantly affected by LWE amount. The amount of bacteria in the egg yogurt was greater than in the control yogurt. Sensory analysis data suggested that color, odor, and texture consistently impacted the overall acceptability of the egg yogurt. The addition of 5% whole egg, which resulted in an increase of 6.28-fold in hardness, increase of 6.1-fold in viscosity, decrease in pH values, and a 5.6% decline in water-holding capacity (WHC). The aroma and flavor of the set yogurt was improved as well. LWE addition significantly increased the protein content and dynamic rheology. More importantly, the addition of LWE increased the protein content of the set yogurt. This investigation demonstrated the feasibility of fabricating LWE-enriched set yogurt and its superior quality compared with the corresponding normal product. It also emphasized the reconstruction of LWE with enhanced properties.
Structural Properties of Casein Micelles with Adjusted Micellar Calcium Phosphate Content
Authors: Ahmadi Elaheh , Markoska Tatijana , Huppertz Thom , Vasiljevic Todor
Published: January 2024

Micellar calcium phosphate (MCP) content of skim milk was modified by pH adjustment followed by dialysis. Turbidity, casein micelle size and partitioning of Ca and caseins between the colloidal and soluble phases of milk were determined. Protein structure was characterised by Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance (1H NMR), whereas organic and inorganic phosphorus were studied by phosphorus-31 nuclear magnetic resonance (31P NMR). The sample with the lowest MCP content (MCP7) exhibited the smallest particle size and turbidity, measuring 83 ± 8 nm and 0.08 ± 0.01 cm−1, respectively. Concentrations of soluble caseins increased with decreasing MCP levels. At ~60% MCP removal, FTIR analysis indicated a critical stage of structural rearrangement and 31P NMR analysis showed an increase in signal intensity for Ca-free Ser-P, which further increased as MCP concentration was further reduced. In conclusion, this study highlighted the importance of MCP in maintaining micellar structure and its impact on the integrity of casein micelle.
Enzymatic Hydrolysis of Water Lentil (Duckweed): An Emerging Source of Proteins for the Production of Antihypertensive Fractions
Authors: Bernier Marie-Ève , Thibodeau Jacinthe , Bazinet Laurent
Published: January 2024

Water lentil (Duckweed), an emerging protein source, is a small floating aquatic plant with agronomic and compositional characteristics rendering it a potential source of bioactive peptides. However, enzymatic hydrolysis of duckweeds has only been carried out to assess the antioxidant and antimicrobial activities of the hydrolysates. The main objectives of this study were to perform enzymatic hydrolysis of duckweed powder utilizing several enzymes and to evaluate the final antihypertensive activity of the fractions. Duckweed powder was efficiently hydrolyzed by pepsin, chymotrypsin, papain and trypsin, with degree of hydrolysis ranging from 3% to 9%, even without prior extraction and concentration of proteins. A total of 485 peptide sequences were identified in the hydrolysates and only 51 were common to two or three hydrolysates. It appeared that phenolic compounds were released through enzymatic hydrolyses and primarily found in the supernatants after centrifugation at concentrations up to 11 mg gallic acid/g sample. The chymotryptic final hydrolysate, the chymotryptic supernatant and the papain supernatant increased the ACE inhibitory activity by more than 6- to 8-folds, resulting in IC50 values ranging between 0.55 to 0.70 mg peptides/mL. Depending on the fraction, the ACE-inhibition was attributed to either bioactive peptides, phenolic compounds or a synergistic effect of both. To the best of our knowledge, this was the first study to investigate the enzymatic hydrolysis of duckweed proteins to produce bioactive peptides with therapeutic applications in mind.
Sustainable In Silico-Supported Ultrasonic-Assisted Extraction of Oligomeric Stilbenoids from Grapevine Roots Using Natural Deep Eutectic Solvents (NADES) and Stability Study of Potential Ready-to-Use Extracts
Authors: Kiene Mats , Zaremba Malte , Januschewski Edwin , Juadjur Andreas , Jerz Gerold , Winterhalter Peter
Published: January 2024

Grapevine roots, as a side-stream of a vineyard, are a sustainable resource for the recovery of oligomeric stilbenoids, such as the bioactive r-viniferin. The aim of this study is to evaluate an in silico-supported method, based on the Conductor-like Screening Model for Real Solvents (COSMO-RS), for selection of environmentally friendly natural deep eutectic solvents (NADES) with regard to the extraction of grapevine roots. The most suitable NADES system for ultrasonic-assisted extraction of r-viniferin was choline chloride/1,2-propanediol. The optimal extraction parameters for r-viniferin were determined using single-factor experiments as follows: choline chloride/1,2-propanediol 1/2 mol/mol, 10 wt% H2O, biomass/NADES ratio 1/10 g/g, and 10 min extraction time. Under optimized conditions, the extraction yield of r-viniferin from grapevine roots reached 76% of the total r-viniferin content. Regarding stability, stilbenoids in choline chloride/1,2-propanediol remained stable during 128 days of storage at ambient temperature. However, fructose/lactic acid-based NADES were observed to degrade stilbenoids; therefore, the removal of the NADES will be of interest, with a suitable method implemented using Amberlite® XAD-16N resin. As green solvents, the NADES have been used as effective and environmentally friendly extractants of stilbenoid-containing extracts from grapevine roots for potential applications in the cosmetic and pharmaceutical industry or as nutraceuticals in the food industry.
Germination Study of Some Protein-Based Gels Obtained from By-Products from the Leather Industry on Tomato and Pepper Seeds
Authors: Cristea Stelica , Niculescu Mihaela-Doina , Perisoara Alina , Ivan Elena , Stanca Maria , Alexe Cosmin-Andrei , Tihauan Bianca-Maria , Olariu Laura
Published: January 2024

This study aimed to evaluate the biostimulant effects of three protein-based gels, GHC 1-B (20% gelatin (GPU-B) obtained by thermal hydrolysis from residual untanned leather and 80% collagen hydrolysates (HCE-B) obtained by alkaline–enzymatic hydrolysis from residual bovine-tanned leather), GHC 2-B (40% keratin hydrolysate (HKU-B) obtained by alkaline–enzymatic hydrolysis from sheep wool + 40% HCE-B + 20% GPU-B), and GHC 3-B (20% GPU-B + 80% hydrolyzed collagen (HPU-B) obtained by thermal and enzymatic hydrolysis from residual untanned leather). A germination study was carried out on pepper and tomato seeds at concentrations of 1%, 3%, and 10%. As a result of the study, it was found that all three protein-based gels showed a stimulatory effect on the tomato seeds at a 1% concentration, where the Gi (germination index) was ˂100%. The GHC 2-B variant had the highest stimulatory effect (Gi-190.23%). Pepper seeds have proven to be more sensitive to the gel’s composition. The concentration at which it proved to be non-inhibitory (Gi–88.29%) was 1% in the case of GHC 2-B. It was found that the presence of hydrolyzed keratin in the composition can be a plus compared to the other two protein gels tested due to its composition, which is richer in phytonutrient compounds (e.g., sulfur molecules).
Aligning Cancer Research Priorities in Europe with Recommendations for Conquering Cancer: A Comprehensive Analysis
Authors: Horgan Denis , Bulcke Marc Van den , Malapelle Umberto , Normanno Nicola , Capoluongo Ettore D. , Prelaj Arsela , Rizzari Carmelo , Stathopoulou Aliki , Singh Jaya , Kozaric Marta , Dube France , Ottaviano Manuel , Boccia Stefania , Pravettoni Gabriella , Cattaneo Ivana , Malats Núria , Buettner Reinhard , Lekadir Karim , de Lorenzo Francesco , Blanc Patricia , Alix-Panabieres Catherine , Badreh Sara , Hofman Paul , Solary Eric , De Maria Ruggero
Published: January 2024

Improvements in cancer care require a new degree of collaboration beyond the purely medical sphere, extending deeply into the world of other stakeholders—preeminently patients but also the other stakeholders in the hardware and software of care. Cancer remains a global health challenge, necessitating collaborative efforts to understand, prevent, and treat this complex disease. To achieve this goal, a comprehensive analysis was conducted, aligning the prioritization of cancer research measures in 13 European countries with 13 key recommendations for conquering cancer in the region. The study utilized a survey involving both patients and citizens, alongside data from IQVIA, a global healthcare data provider, to assess the availability and access to single-biomarker tests in multiple European countries. The results revealed a focused approach toward understanding, preventing, and treating cancer, with each country emphasizing specific research measures tailored to its strengths and healthcare objectives. This analysis highlights the intricate relationship between research priorities, access to biomarker tests, and financial support. Timely access to tests and increased availability positively influence research areas such as cancer prevention, early detection, ageing, and data utilization. The alignment of these country-specific measures with 13 recommendations for conquering cancer in Europe underscores the importance of tailored strategies for understanding, preventing, and treating cancer.
Physical Activity and Its Potential Determinants in Obese Children and Adolescents under Specialist Outpatient Care—A Pilot Cross-Sectional Study
Authors: Kawalec Anna , Mozrzymas Renata , Domżol Agata , Zachurzok Agnieszka , Szczepańska Maria , Noczyńska Anna , Zwolińska Danuta
Published: January 2024

(1) Background: this study aimed to assess the physical activity of obese pediatric patients under specialized outpatient care and its potential determinants. (2) Methods: A total of 83 subjects aged 7–18 years with simple obesity and their parents were enrolled. Data were collected with the use of physical activity questionnaires (PAQs) for children and adolescents and additional questions concerning selected socio-demographic characteristics. (3) Results: The mean final PAQ score was 2.09 ± 0.69. The most frequently chosen types of physical activity included walking, gymnastics, and jogging or running. We found a weak correlation inversely proportional between the child’s age and mean final PAQ score (r = −0.25; p = 0.02). Younger children were more active during lunchtime at school and after school compared to adolescents (p = 0.03 and p = 0.04). The final PAQ score differed according to the place of residence; the lowest score was obtained by subjects living in cities >100,000 inhabitants (p = 0.025). We found a positive correlation between PAQ-Ch score and the father’s physical activity, and between PAQ-A score and the mother’s education. (4) Conclusions: The physical activity of obese pediatric patients is low, particularly in adolescents. It seems that age and place of residence have an impact on the physical activity of obese children and adolescents. The PAQs used in this study are useful in physical activity assessment and identification of time segments during the day in which activity might be improved. However, this requires confirmation in a larger group of pediatric patients.
Primary Care Professionals’ Empathy and Its Relationship to Approaching Patients with Risky Alcohol Consumption
Authors: Pérula-Jiménez Celia , Romero-Rodríguez Esperanza , Fernández-Solana Jessica , Fernández-García José Ángel , Parras-Rejano Juan Manuel , Pérula-de Torres Luis Ángel , González-de la Rubia Ana , González-Santos Josefa ,   Collaborative Group ALCO-AP20 Study
Published: January 2024

The aim of this study was to estimate the level of empathy among primary care (PC) health professionals and its relationship with their approach to patients at risk due to alcohol consumption. This is an observational, descriptive, and multicenter study that included 80 PHC professionals. The professionals completed a questionnaire comprising socio-occupational questions and inquiries regarding their actions when dealing with patients suspected of risky alcohol consumption. The Jefferson Scale of Empathy was used to measure their level of empathy and was completed by 80 professionals, of whom 57.5% were family physicians, 10% were nurses, and 32.5% were family- and community-medicine residents. The mean age was 39.5 ± 13.1 (SD) (range of 24–65 years) and 71.3% were females. The mean empathy level score was 112.9 ± 11.1 (95% CI: 110.4–115.4; range: 81–132 points). Actions that stood out for their frequency were providing health advice in the general population, offering advice to pregnant women, and recommending abstinence to users of hazardous machinery or motor vehicles. The level of empathy was associated with age (p = 0.029), the health center’s scope (p = 0.044), systematic alcohol exploration (p = 0.034), and follow-ups for patients diagnosed with risky consumption (p = 0.037). The mean score obtained indicated a high level of empathy among professionals. Professionals with greater empathy more frequently conducted systematic screening for risky alcohol consumption.
Four-Dimensional Flow Echocardiography: Blood Speckle Tracking in Congenital Heart Disease: How to Apply, How to Interpret, What Is Feasible, and What Is Missing Still
Authors: Cantinotti Massimiliano , Marchese Pietro , Franchi Eliana , Santoro Giuseppe , Assanta Nadia , Giordano Raffaele
Published: January 2024

Blood speckle tracking echocardiography (BSTE) is a new, promising 4D flow ultrafast non-focal plane imaging technique. The aim of the present investigation is to provide a review and update on potentialities and application of BSTE in children with congenital heart disease (CHD) and acquired heart disease. A literature search was performed within the National Library of Medicine using the keywords “echocardiography”, “BST”, and “children”. The search was refined by adding the keywords “ultrafast imaging”, “CHD”, and “4D flow”. Fifteen studies were finally included. Our analysis outlined how BSTE is highly feasible, fast, and easy for visualization of normal/abnormal flow patterns in healthy children and in those with CHD. BSTE allows for visualization and basic 2D measures of normal/abnormal vortices forming the ventricles and in the main vessel. Left ventricular vortex characteristics and aortic flow patterns have been described both in healthy children and in those with CHD. Complex analysis (e.g., energy loss, vorticity, and vector complexity) are also highly feasible with BSTE, but software is currently available only for research. Furthermore, current technology allows for BSTE only in neonates and low-weight children (e.g., <40 kg). In summary, the feasibility and potentialities of BSTE as a complementary diagnostic tool in children have been proved; however, its systemic use is hampered by the lack of (i) accessible tools for complex quantification and for acquisition at all ages/weight, (ii) data on the diagnostic/prognostic significance of BSTE, and (iii) consensus/recommendation papers indicating when and how BSTE should be employed.
Unraveling the Seismic Source in Archaeoseismology: A Combined Approach on Local Site Effects and Geochemical Data Integration
Authors: Bottari Carla , Capizzi Patrizia , Sortino Francesco
Published: January 2024

Archaeoseismological research often deals with two unresolved questions: the magnitude and level of damage caused by past earthquakes, and the precise location of the seismic source. We propose a comprehensive review of an integrated approach that combines site effects with the analysis of geochemical data in the field of archaeoseismology. This approach aims to identify active buried faults potentially related to the causative seismic source and provide insights into earthquake parameters. For each integrated method, we report the foundational principles, delineation of theoretical field procedures, and exemplification through two case studies. Site effects analysis in archaeoseismology assumes a pivotal role in unraveling historical seismic occurrences. It enables estimating the earthquake magnitude, assessing the seismotectonic patterns, and determining the resulting damage level. Valuable data related to earthquake parameters can be extracted by analyzing vibration frequencies and acceleration measurements from structures within archaeological sites. This information is instrumental in characterizing seismic events, evaluating their impact on ancient structures, and enhancing our understanding of earthquake hazards within the archaeological context. Geochemical investigations supply indispensable tools for identifying buried active faults. The analysis of fluids and gases vented in proximity to faults yields valuable insights into their nature, activity, and underlying mechanisms. Faults often manifest distinctive geochemical imprints, enabling the differentiation between tectonically active and volcanically related fault systems. The presence of specific gases can further serve as indicators of the environmental conditions surrounding these fault networks. Integrating site effects analysis and geochemical investigations within archaeoseismological research is crucial to improving our understanding of unknown past earthquakes. Moreover, it enhances the seismic hazard assessment of the region under study.
Multi-Feature Uncertainty Analysis for Urban-Scale Hypothetical 3D Reconstructions: Piazza delle Erbe Case Study
Authors: Apollonio Fabrizio Ivan , Fallavollita Federico , Foschi Riccardo , Smurra Rosa
Published: January 2024

For the hypothetical reconstruction of architectural heritage, there are still no scientific standards of reference concerning their sharing and documentation. Recent international initiatives established the basis to address this problem; however, still, much work needs to be done in order to systematise good practices for the process of reconstruction and its dissemination. This contribution aims to take a step forward in the analysis and visualisation of uncertainty. Some authors have suggested various approaches to visualise uncertainty for single buildings; however, case studies at the urban scale are rarely investigated. This research proposes an improved source-based multi-feature approach aimed at analysing and visualising (through false-colour shading) the uncertainty of hypothetical 3D digital models of urban areas. The assessment of uncertainty is also quantified qualitatively by using newly defined formulas which calculate the average uncertainty weighted on the volume of the 3D model. This methodology aims to improve the objectiveness, unambiguity, transparency, reusability, and readability of hypothetical reconstructive 3D models, and its use is exemplified in the case study of the hypothetical reconstruction of Piazza delle Erbe in Verona, a project presented in the form of a docufilm at EXPO 2015 in Milan.
Comparative Transcriptome Analysis Reveals Changes in Gene Expression Associated with Anthocyanin Metabolism in Begonia semperflorens under Light Conditions
Authors: Zhao Kunkun , Liu Airong , Zhang Yuanbing , Liu Weixin , Zhao Zhimin , Yang Shuyue
Published: January 2024

Anthocyanins, recognized as stress indicators, particularly under high-light conditions, play a pivotal role in plant stress responses. The advent of transcriptomics has opened avenues to elucidate the mechanisms underlying high light-induced anthocyanin biosynthesis. This study delved into transcriptomic changes in Begonia semperflorens leaves under varying light intensities: 950–9600 lx (TL_100), 6800–7000 lx (HS_75), and 4300–4500 lx (LS_25). To confirm the expression profiles of the key genes, we chose 12 critical genes associated with anthocyanin production for quantitative reverse transcription PCR (qRT-qPCR) analysis. Following this, we measured the levels of anthocyanins to substantiate the findings from the gene expression analysis. The transcriptome assembly in this study was extensive, yielding 43,038 unigenes that collectively spanned about 49.83 million base pairs, with an average unigene length of 1157 bp and an N50 value of 1685 bp. This assembly facilitated a thorough functional annotation across seven distinct protein databases, leading to the classification of 16,363 unigenes into 58 different families of transcription factors. Our comparative analysis of the transcriptomes highlighted a substantial number of differentially expressed genes (DEGs): 5411 DEGs between HS_75 and TL_100 conditions, with 3078 showing increased expression and 2333 showing decreased expression; 4701 DEGs between LS_25 and TL_100, consisting of 2648 up-regulated and 2053 down-regulated genes; and 6558 DEGs between LS_25 and HS_75, with 3032 genes up-regulated and 3526 down-regulated. These DEGs were significantly involved in critical pathways, such as anthocyanin synthesis, plant hormone signaling, and other regulatory mechanisms. This study suggests that genes, including F3′H, MYB102, and SWEET1, could play vital roles in regulating anthocyanin synthesis in response to various light conditions, potentially impacting the expression levels of other genes, like WRKYs, ATHB12, and those similar to HSP.
Computational Modeling of High-Speed Flow of Two-Phase Hydrogen through a Tube with Abrupt Expansion
Authors: Matveev Konstantin I.
Published: January 2024

Hydrogen can become a prevalent renewable fuel in the future green economy, but technical and economic hurdles associated with handling hydrogen must be overcome. To store and transport hydrogen in an energy-dense liquid form, very cold temperatures, around 20 K, are required. Evaporation affects the achievable mass flow rate during the high-speed transfer of hydrogen at large pressure differentials, and accurate prediction of this process is important for the practical design of hydrogen transfer systems. Computational fluid dynamics modeling of two-phase hydrogen flow is carried out in the present study using the volume-of-fluid method and the Lee relaxation model for the phase change. Suitable values of the relaxation time parameter are determined by comparing numerical results with test data for high-speed two-phase hydrogen flows in a configuration involving a tube with sudden expansion, which is common in practical systems. Simulations using a variable outlet pressure are conducted to demonstrate the dependence of flow rates on the driving pressure differential, including the attainment of the critical flow regime. Also shown are computational results for flows with various inlet conditions and a fixed outlet state. Field distributions of the pressure, velocity, and vapor fractions are presented for several flow regimes.
Microplastic Contamination of Fine-Grained Sediments and Its Environmental Driving Factors along a Lowland River: Three-Year Monitoring of the Tisza River and Central Europe
Authors: Balla Alexia , Teofilovic Vesna , Kiss Tímea
Published: January 2024

The hydro-geomorphological background in microplastic (MP) deposition and mobilization is often neglected, though the sampling environment is the key point in a monitoring scheme. The aim of the study was to analyze the environmental driving factors of MP transport over three years (2020–2022) along a 750 km-long section of the Tisza River, Central Europe. The mean MP content of the fresh clayey sediments was 1291 ± 618 items/kg in 2020, and then it decreased (2021: 730 ± 568 items/kg; 2022: 766 ± 437 items/kg). The upstream and downstream sections were the most polluted due to improper local sewage treatment. In 2020, 63% of the sites were hotspot (≥2000 items/kg), but their number decreased to one-third in 2021 and 2022. MP pollution is influenced by highly variable environmental factors. (1) The geomorphological setting of a site is important, as most of the hotspots are on side bars. (2) The tributaries convey MP pollution to the Tisza River. (3) The bankfull or higher flood waves effectively rearrange the MP pollution. (4) The dams and their operation influence the downstream trend of MP pollution in the reservoir. (5) Downstream of a dam, the clear-water erosion increases the proportion of the pristine sediments; thus, the MP concentration decreases.
Sophoridine Counteracts Obesity via Src-Mediated Inhibition of VEGFR Expression and PI3K/AKT Phosphorylation
Authors: Sun Jingchun , Wang Xiaoting , He Yulin , Tian Xuekai , Yuan Tiantian , Yang Gongshe , Yu Taiyong
Published: January 2024

Sophoridine (SRP) is a natural quinolizidine alkaloid found in many traditional Chinese herbs, though its effect on adipose tissue is unclear. We improved serum lipid levels by administering SRP by gavage in high-fat diet (HFD)-fed C57BL/6 mice. After 11 weeks, SRP supplementation significantly reduced body weight gain and improved glucose homeostasis, while reducing subcutaneous fat and liver weight. SRP also inhibited cell proliferation and differentiation of 3T3-L1 cells. Proteomics analysis revealed that SRP inhibits adipocyte differentiation by interacting with Src, thereby suppressing vascular endothelial growth factor receptor 2 (VEGFR2) expression and PI3K/AKT phosphorylation. This study provides an empirical basis for the treatment of obesity with small molecules.
The Impact of Obesity on Diabetes Onset and Neovascularization in Mouse Models of Metabolic Stress
Authors: Meda Venkata Sai Pranathi , Li Hainan , Xu Liping , Wang Jie-Mei
Published: January 2024

Animal models of metabolic disorders are essential to studying pathogenic mechanisms and developing therapies for diabetes, but the induction protocols vary, and sexual dimorphism often exists. In a chronic diabetic model of diet-induced obesity (DIO) and low-dose streptozotocin (STZ)-induced hyperglycemia, blood glucose and lipid profiles were measured. The high-fat (HF) diet damaged insulin sensitivity and increased triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol, and liver lipid deposition. STZ increased blood glucose and liver fibrosis with less effects on blood lipids or liver lipid deposition. The combination of DIO and STZ treatments led to significant liver lipid deposition and fibrosis. Female mice showed delayed body weight gain on HF diet and resisted STZ-induced hyperglycemia. However, once they developed DIO, which occurs around 26 weeks of HF diet, the female mice were prone to STZ-induced hyperglycemia. In hindlimb ischemia, male mice in the DIO-STZ group showed significantly worse neovascularization compared with DIO or STZ groups. The DIO-STZ females showed significantly worse recovery than the DIO-STZ males. Our observations suggest that DIO-STZ is a plausible model for studying metabolic and cardiovascular disorders in obesity and diabetes. Moreover, the findings in female animals stress the need to assess sexual dimorphism and investigate the underlying mechanisms that contribute to the worse vasculopathy manifestations in females in metabolic models.
Effects of Rumen-Protected L-Tryptophan Supplementation on Productivity, Physiological Indicators, Blood Profiles, and Heat Shock Protein Gene Expression in Lactating Holstein Cows under Heat Stress Conditions
Authors: Jo Jang-Hoon , Jalil Ghassemi Nejad , Kim Won-Seob , Moon Jun-Ok , Lee Sung-Dae , Kwon Chan-Ho , Lee Hong-Gu
Published: January 2024

In this study, we examined the effects of rumen-protected L-tryptophan supplementation on the productivity and physiological metabolic indicators in lactating Holstein cows under heat stress conditions. The study involved eight early lactating Holstein cows (days in milk = 40 ± 9 days; milk yield 30 ± 1.5 kg/day; parity 1.09 ± 0.05, p < 0.05), four cows per experiment, with environmentally controlled chambers. In each experiment, two distinct heat stress conditions were created: a low-temperature and low-humidity (LTLH) condition at 25 °C with 35–50% humidity and a high-temperature and high-humidity (HTHH) condition at 31 °C with 80–95% humidity. During the adaptation phase, the cows were subjected to LTLH and HTHH conditions for 3 days. This was followed by a 4-day heat stress phase and then by a 7-day phase of heat stress, which were complemented by supplementation with rumen-protected L-tryptophan (ACT). The findings revealed that supplementation with ACT increased dry matter intake as well as milk yield and protein and decreased water intake, heart rate, and rectal temperature in the HTHH group (p < 0.05). For plateletcrit (PCT, p = 0.0600), the eosinophil percentage (EOS, p = 0.0880) showed a tendency to be lower, while the monocyte (MONO) and large unstained cells (LUC) amounts were increased in both groups (p < 0.05). Albumin and glucose levels were lower in the HTHH group (p < 0.05). The gene expressions of heat shock proteins 70 and 90 in the peripheral blood mononuclear cells were higher in the ACT group (HTHH, p < 0.05). These results suggest that ACT supplementation improved productivity, physiological indicators, blood characteristics, and gene expression in the peripheral blood mononuclear cells of early lactating Holstein cows under heat-stress conditions. In particular, ACT supplementation objectively relieved stress in these animals, suggesting that L-tryptophan has potential as a viable solution for combating heat-stress-induced effects on the cattle in dairy farming.
Salt-Induced Early Changes in Photosynthesis Activity Caused by Root-to-Shoot Signaling in Potato
Authors: Pecherina Anna , Dimitrieva Anastasia , Mudrilov Maxim , Ladeynova Maria , Zanegina Daria , Brilkina Anna , Vodeneev Vladimir
Published: January 2024

Salinity is one of the most dangerous types of stress in agriculture. Acting on the root, salinity causes changes in physiological processes in the shoot, especially photosynthesis, which is crucial for plant productivity. In our study, we used potato plants, the most important crop, to investigate the role of salt-induced signals in changes in photosynthesis activity. We found a salt-induced polyphasic decrease in photosynthesis activity, and the earliest phase started several minutes after salt addition. We found that salt addition triggered rapid hydraulic and calcium waves from root to shoot, which occurred earlier than the first phase of the photosynthesis response. The inhibition of calcium signals by lanthanum decreased with the formation of rapid changes in photosynthesis. In addition to this, a comparison of the characteristic times of signal propagation and the formation of a response revealed the role of calcium waves in the modulation of rapid changes in photosynthesis. Calcium waves are activated by the ionic component of salinity. The salt-induced decrease in transpiration corresponds in time to the second phase of the photosynthetic response, and it can be the cause of this change. The accumulation of sodium in the leaves occurs a few hours after salt addition, and it can be the cause of the long-term suppression of photosynthesis. Thus, salinity modulates photosynthetic activity in plants in different ways: both through the activation of rapid distant signals and by reducing the water input and sodium accumulation.
Potassium Ions Decrease Mitochondrial Matrix pH: Implications for ATP Production and Reactive Oxygen Species Generation
Authors: Naima Jannatul , Ohta Yoshihiro
Published: January 2024

Potassium (K+) is the most abundant cation in the cytosol and is maintained at high concentrations within the mitochondrial matrix through potassium channels. However, many effects of K+ at such high concentrations on mitochondria and the underlying mechanisms remain unclear. This study aims to elucidate these effects and mechanisms by employing fluorescence imaging techniques to distinguish and precisely measure signals inside and outside the mitochondria. We stained the mitochondrial matrix with fluorescent dyes sensitive to K+, pH, reactive oxygen species (ROS), and membrane potential in plasma membrane-permeabilized C6 cells and isolated mitochondria from C6 cells. Fluorescence microscopy facilitated the accurate measurement of fluorescence intensity inside and outside the matrix. Increasing extramitochondrial K+ concentration from 2 mM to 127 mM led to a reduction in matrix pH and a decrease in the generation of highly reactive ROS. In addition, elevated K+ levels electrically polarized the inner membrane of the mitochondria and promoted efficient ATP synthesis via FoF1-ATPase. Introducing protons (H+) into the matrix through phosphate addition led to further mitochondrial polarization, and this effect was more pronounced in the presence of K+. K+ at high concentrations, reaching sub-hundred millimolar levels, increased H+ concentration within the matrix, suppressing ROS generation and boosting ATP synthesis. Although this study does not elucidate the role of specific types of potassium channels in mitochondria, it does suggest that mitochondrial K+ plays a beneficial role in maintaining cellular health.