bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and safety of the Chaixiong Qiwei granule for tension-type headache: study protocol for a randomised controlled trial
Authors: Shen Wei , Fan Xueming , Fu Guojing , Liu Hongxi , Liang Xiao , Wei Jingjing , Sun Linjuan , Zhang Lu , Chi Xiansu , Zhang Yunling
Published: December 2023

Introduction

Tension-type headache (TTH) is the most prevalent headache disorder worldwide. Although current treatments for TTH are beneficial, they are not without adverse effects. Chaixiong Qiwei granule (CXQW) is an experienced prescription medicine for TTH management. This study will evaluate the efficacy and safety of CXQW for the treatment of TTH.

Methods and analysis

This study will be a multicentre, randomised, double-blind, placebo-controlled trial. A total of 148 eligible participants will be divided into the intervention (CXQW treatment) and control (placebo treatment) groups. The primary outcome will be the reduction in the number of headache days (headache-days reduction) within 9–12 weeks after randomisation, while secondary outcomes will include the number of headache days, headache intensity, responder rate, drug consumption for acute treatment, quality of life and symptoms related to traditional Chinese medicine use based on a symptom-observation table. This protocol describes the design of the randomised controlled trial.

Ethics and dissemination

The study design was approved by the Institutional Review Board of Human Research at Xiyuan Hospital, China Academy of Chinese Medical Sciences (No. 2020XLA030-2).

Trial registration number

ChiCTR2100042514.
Understanding medication-related burden from patient perspectives: a qualitative study testing the applicability of the conceptual model among chronically ill outpatients in Finland
Authors: Mikkola Heidi , Dimitrow Maarit , Hämeen-Anttila Katri , Laukkanen Emilia , Airaksinen Marja
Published: December 2023

Objectives

Disease self-management and medication therapy can cause burden to patients that can influence adherence. The conceptual model ‘patients’ lived experience with medicine’ (PLEM) brings new insights into medication-related burden (MRB) from patient perspective. This study aimed to test the applicability of the PLEM model by interviewing chronically ill patients in Finland and to investigate the MRB experienced by the Finnish patients.

Design

Focus group discussion study conducted online via Zoom. Directed qualitative content analysis guided by the PLEM model.

Setting

Outpatient primary care in Finland.

Participants

Chronically ill outpatients (n=14) divided into five focus groups according to their chronic condition: asthma (n=3), heart disease (n=3), diabetes (n=6), intestinal disease (n=2).

Results

Our findings were mainly in line with the PLEM model although some new contributing factors to MRB emerged. In general, the participants were satisfied with their medication, and that it enabled them to live normal lives. The most common causes of MRB were medication routines and the healthcare system. The participants introduced two new aspects contributing to MRB: medication-related environmental anxiety associated with the waste resulting from medicine use, and the effect of medication use on their working life. Our findings are consistent with previous findings that a higher level of MRB may lead to independently modifying the medication regimen or not taking the medicine.

Conclusions

Our findings provide further evidence that the PLEM model is an applicable tool also in the Finnish context for gaining better understanding of MRB in chronically ill patients self-managing their long-term medications. The model provides a promising tool to understand the connection between MRB and the rationale for not always taking medicines as prescribed. Further research is needed to explore the potential of the model in extending patient perspectives in chronic disease management.
Knowledge and attitudes on attention deficit hyperactivity disorder (ADHD) among school teachers in Anuradhapura district, Sri Lanka: a descriptive cross-sectional study
Authors: Wijerathna Navoda , Wijerathne Charith , Wijeratne Himeshika , Wijesiri Chathuri , Wijerathna Randika , Wijerathna Windhya , Warnasekara Janith , Agampodi Thilini , Rajapakse Shashanka
Published: December 2023

Objective

This study aimed to assess the knowledge and attitude of school teachers from a rural district in a developing country towards children with attention deficit hyperactivity disorder (ADHD).

Design

A population-based descriptive cross-sectional study was conducted using probability proportionate to size cluster sampling.

Setting

This study was conducted in 21 government schools in Anuradhapura district, Sri Lanka.

Participants

The study sample consisted of 458 teachers with a mean age of 41 completed years ranging from 24 to 59 years.

Main outcome measurements

We assessed knowledge, attitudes and sources of information on ADHD using the self-administered, validated Knowledge of Attention Deficit Disorder Scale (KADDS), Teacher Attitudes Towards Inclusion Scale (TAIS) and the Perceived and Actual Sources of Information questionnaires.

Results

The median KADDS score was 11 (IQR 8–14) and 45.5% (n=208) of participants lacked sufficient knowledge. Percentages of the correct answers, misconceptions and ‘don’t know’ responses were 28.2%, 24.5% and 45.5%, respectively. The majority of teachers believed that the children with ADHD generally experience more problems in novel situations than in familiar situations (63.5%), a diagnosis of ADHD by itself makes a child eligible for placement in special education (61.1%), and children with ADHD do not often have difficulties organising tasks and activities (61.1%). Some participants (12%) stated that punishment would improve the outcome of the children with ADHD. The attitudes were positive with TAIS 1 and 2 median scores of 46 (IQR 36–58) and 49 (IQR 40–59). The majority of participants relied on informal knowledge gained through their personal experience in the classroom on ADHD (n=337, 76%). The majority of teachers (n=300, 67.7%) preferred to be educated through seminars.

Conclusions

School teachers possess a positive attitude. However, they have poor knowledge and significant misconceptions regarding ADHD which may affect the identification and management.
Single-cell transcriptomic analysis deciphers heterogenous cancer stem-like cells in colorectal cancer and their organ-specific metastasis
Authors: Li Rui , Liu Xuefei , Huang Xudong , Zhang Di , Chen Ziming , Zhang Jialiang , Bai Ruihong , Zhang Shaoping , Zhao Hongzhe , Xu Zilan , Zeng Lingxing , Zhuang Lisha , Wen Shujuan , Wu Shaojia , Li Mei , Zuo Zhixiang , Lin Junzhong , Lin Dongxin , Zheng Jian
Published: December 2023

Objective

Metastasis is the major cause of cancer death. However, what types of heterogenous cancer cells in primary tumour and how they metastasise to the target organs remain largely undiscovered.

Design

We performed single-cell RNA sequencing and spatial transcriptomic analysis in primary colorectal cancer (CRC) and metastases in the liver (lCRC) or ovary (oCRC). We also conducted immunofluorescence staining and functional experiments to examine the mechanism.

Results

Integrative analyses of epithelial cells reveal a stem-like cell cluster with highprotein tyrosine phosphatase receptor type O(PTPRO) andachaete scute-like 2(ASCL2) expression as the metastatic culprit. This cell cluster comprising distinct subpopulations shows distinct liver or ovary metastatic preference. Population 1 (P1) cells with highdelta-like ligand 4 (DLL4)andMAF bZIP transcription factor A (MAFA)expression are enriched in primary CRC and oCRC, thus may be associated with ovarian metastasis. P3 cells having a similar expression pattern as cholangiocytes are found mainly in primary CRC and lCRC, presuming to be likely the culprits that specifically metastasise to the liver. Stem-like cells interacted with cancer-associated fibroblasts and endothelial cells via the DLL4-NOTCH signalling pathway to metastasise from primary CRC to the ovary. In the oCRC microenvironment, myofibroblasts provide cancer cells with glutamine and perform a metabolic reprogramming, which may be essential for cancer cells to localise and develop in the ovary.

Conclusion

We uncover a mechanism for organ-specific CRC metastasis.
Multicenter randomized controlled trial of neoadjuvant chemoradiotherapy alone or in combination with pembrolizumab in patients with resectable or borderline resectable pancreatic adenocarcinoma
Authors: Katz Matthew H G , Petroni Gina R , Bauer Todd , Reilley Matthew J , Wolpin Brian M , Stucky Chee-Chee , Bekaii-Saab Tanios S , Elias Rawad , Merchant Nipun , Dias Costa Andressa , Lenehan Patrick , Cardot-Ruffino Victoire , Rodig Scott , Pfaff Kathleen , Dougan Stephanie K , Nowak Jonathan Andrew , Varadhachary Gauri R , Slingluff Craig L , Rahma Osama
Published: December 2023

Background

Pancreatic ductal adenocarcinoma (PDAC) is a challenging target for immunotherapy because it has an immunosuppressive tumor microenvironment. Neoadjuvant chemoradiotherapy can increase tumor-infiltrating lymphocyte (TIL) density, which may predict overall survival (OS). We hypothesized that adding programmed cell death protein 1 (PD-1) blockade to chemoradiotherapy would be well tolerated and increase TILs among patients with localized PDAC.

Methods

Patients were randomized 2:1 to Arm A (receiving pembrolizumab plus chemoradiotherapy (capecitabine and external beam radiation)) or Arm B (receiving chemoradiotherapy alone) before anticipated pancreatectomy. Primary endpoints were (1) incidence and severity of adverse events during neoadjuvant therapy and (2) density of TILs in resected tumor specimens. TIL density was assessed using multiplexed immunofluorescence histologic examination.

Results

Thirty-seven patients were randomized to Arms A (n=24) and B (n=13). Grade ≥3 adverse events related to neoadjuvant treatment were experienced by 9 (38%) and 4 (31%) patients in Arms A and B, respectively, with one patient experiencing dose-limiting toxicity in Arm A. Seventeen (71%) and 7 (54%) patients in Arms A and B, respectively, underwent pancreatectomy. Median CD8+T-cell densities in Arms A and B were 67.4 (IQR: 39.2–141.8) and 37.9 (IQR: 22.9–173.4) cells/mm2, respectively. Arms showed no noticeable differences in density of CD8+Ki67+, CD4+, or CD4+FOXP3+regulatory T cells; M1-like and M2-like macrophages; or granulocytes. Median OS durations were 27.8 (95% CI: 17.1 to NR) and 24.3 (95% CI: 12.6 to NR) months for Arms A and B, respectively.

Conclusions

Adding pembrolizumab to neoadjuvant chemoradiotherapy was safe. However, no convincing effect on CD8+TILs was observed.
Increased mortality in patients with RA-associated interstitial lung disease: data from a French administrative healthcare database
Authors: Juge Pierre-Antoine , Wémeau-Stervinou Lidwine , Ottaviani Sebastien , Desjeux Guillaume , Zhuo Joe , Vannier-Moreau Virginie , Flipo René-Marc , Crestani Bruno , Dieudé Philippe
Published: December 2023

Objectives

Interstitial lung disease (ILD) is a severe extra-articular manifestation of rheumatoid arthritis (RA). The objectives of this study were to estimate mortality rate in patients with RA-ILD and identify factors affecting mortality.

Methods

Data from a French national claims database (Système National des Données de Santé) from 2013 to 2018 were analysed. Adults with an RA diagnosis (International Classification of Diseases (ICD)-10 codes M05, M06.0, M06.8 and M06.9) were included. ILD diagnosis was defined with ICD-10 code J84. Mortality rates were compared between patients with RA with and without ILD, using Cox proportional hazards regression, after matching 1:1 for age, sex, age at RA-ILD onset and RA duration.

Results

Among 173 132 patients with RA, 4330 (3%) also had ILD (RA-ILD). After matching, RA-ILD was associated with an increased mortality rate (HR 3.4, 95% CI 3.1 to 3.9). The HR for mortality was greater for: patients aged <75 years (HR 4.8, 95% CI 3.9 to 5.9) versus ≥75 years (HR 3.0, 95% CI 2.6 to 3.5); patients with ILD onset occurring before RA onset (HR 8.4, 95% CI 5.5 to 13.0) versus ILD onset occurring after RA onset (HR 2.9, 95% CI 2.6 to 3.3); and men (HR 5.2, 95% CI 4.4 to 6.2) versus women (HR 3.6, 95% CI 3.0 to 4.2).

Conclusion

In this nationwide cohort study, RA-ILD was associated with increased mortality rate (vs in patients with RA without ILD), notably for those aged <75 years, those whose ILD preceded RA onset and men.
Good involutions of generalized Alexander quandles
Authors: Taniguchi Yuta
Published: December 2023

Quandles with good involutions, which are called symmetric quandles, can be used to define invariants of unoriented knots and links. In this paper, we determine the necessary and sufficient condition for good involutions of a generalized Alexander quandle to exist. Moreover, we classify all good involutions of a connected generalized Alexander quandle up to symmetric quandle isomorphisms.
Concordance invariant Υ for balanced spatial graphs using grid homology
Authors: Kubota Hajime
Published: December 2023

The [Formula: see text] invariant is a concordance invariant using knot Floer homology. Földvári [The knot invariant [Formula: see text] using grid homologies, J. Knot Theory Ramifications 30(7) (2021) 2150051] gives a combinatorial restructure of it using grid homology. We extend the combinatorial [Formula: see text] invariant for balanced spatial graphs. Regarding links as spatial graphs, we give an upper and lower bound for the [Formula: see text] invariant when two links are connected by a cobordism. Also, we show that the combinatorial [Formula: see text] invariant is a concordance invariant for knots.
Convolutional LSTM-Attention Based Encoder–Decoder Neural Network for Prediction of Chaotic Vibrations of Multi-Dimensional Dynamic Systems
Authors: Wang Luyao , Dai Liming , Zhao Haixing , Fang Pan
Published: December 2023

The present research proposes a convolutional long-short term memory (ConvLSTM) with an attention mechanism (AM) model, termed as ConvLSTM-AM, to conduct prediction of chaotic vibrations of multi-dimensional dynamic systems. The proposed data-driven model is based on an encoder–decoder architecture where the lengths of inputs and outputs are variable. Different from other conventional benchmarks which consider the temporal correlation solely to deal with the chaotic sequences, this research work takes the spatial information into account. In this sense, the ConvLSTM is adopted as an encoder to acquire useful chaotic spatiotemporal patterns and retain long-term successive dependencies. LSTM and AM in this research are taken as the main structures of the procedure in decoding, in which the LSTM is used as the further temporal processor and AM is stacked on the top to exploit more salient information of the historical data. Among that, a residual connection between the outputs of LSTM and the information of attention is considered in AM to prevent gradient vanishment. Two datasets of chaotic vibrations of multi-dimensional systems are employed to adequately illustrate the effectiveness and feasibility of the proposed model. Besides, five conventional benchmarks are built to demonstrate the advantages of the proposed model in terms of both training and generalization performance. As found in the research, the training time is reduced with lower testing loss in comparing with the other five counterparts, as the spatial information introduced expedites the training convergence. The present research provides a useful guidance for predicting and analysing chaotic vibrations of multi-dimensional dynamic systems.
A Novel Viscoelastic-Friction Model for the Multiphase Hysteretic Behavior of Aluminum Foam/Polyurethane Interpenetrating Phase Composite Damper
Authors: liu Shaobo , Li Aiqun , Jia Caihong , Su Yi , Fang Zhao
Published: December 2023

An aluminum foam/polyurethane interpenetrating phase composite damper (AF/ PUCD) can perform multi-functional energy dissipation to address different seismic hazards due to its multiphase hysteretic behavior. To improve the design of AF/PUCDs in engineering structures, a highly effective model based on the real deformation of an AF/PUCD is needed to describe its multiphase hysteretic behavior. In this paper, a novel viscoelastic-friction model composed of a viscoelastic component and a friction component is constructed. The hysteretic responses in each phase under various external excitations are described through the different combinations of the viscoelastic component and friction component. The unknown model parameters are identified through the Universal Global Algorithm (UGO). The model results are compared with the experimental results and the results from the Modified Bouc–Wen model and Optimum model. The comparative results show that the viscoelastic-friction model has a higher accuracy in capturing the multiphase hysteresis of AF/PUCD and predicting the boundary of each phase when the AF/PUCD is subjected to various cyclic excitations. Therefore, the viscoelastic-friction model is a good candidate for the design of AF/PUCDs applied in vibration control structures.
Stability in Parametric Resonance of a Controlled Stay Cable with Time Delay
Authors: Peng Jian , Xia Hui , Sun Hongxin , Lenci Stefano
Published: December 2023

The stability of the parametric resonance of a controlled stay cable with time delay is investigated. The in-plane nonlinear equations of motion are initially determined via the Hamilton principle. Then, utilizing the method of multiple scales, the modulation equations that govern the nonlinear dynamics are obtained. These equations are then utilized to investigate the effect of time delays on the amplitude and frequency-response behavior and, subsequently, on the stability of the parametric resonance of the controlled cable, that it is shown to depend on the excitation amplitude and the commensurability of the delayed-response frequency to the excitation frequency. The stability region of the parametric resonance is shifted, and the effects of control on the cable become worse by increasing time delay. The work plays a guiding role in the parametric design of the control system for stay cables.
Algorithms for the Uniqueness of the Longest Common Subsequence
Authors: Wang Yue
Published: December 2023

Given several number sequences, determining the longest common subsequence is a classical problem in computer science. This problem has applications in bioinformatics, especially determining transposable genes. Nevertheless, related works only consider how to find one longest common subsequence. In this paper, we consider how to determine the uniqueness of the longest common subsequence. If there are multiple longest common subsequences, we also determine which number appears in all/some/none of the longest common subsequences. We focus on four scenarios: (1) linear sequences without duplicated numbers; (2) circular sequences without duplicated numbers; (3) linear sequences with duplicated numbers; (4) circular sequences with duplicated numbers. We develop corresponding algorithms and apply them to gene sequencing data.
Blockchain in Smart Cities: A Bibliometric Analysis and Overview
Authors: Karger Erik , Brée Tim , Ziolkowski Rafael , Jagals Marvin , Ahlemann Frederik
Published: December 2023

Since the 2000s, the interest around smart cities continues to grow due to the increasing digitalization, urbanization, and marketing of the smart city idea by tech companies. A central characteristic of smart cities is their use of digital technologies. One of these technologies that is increasingly applied in smart cities is the blockchain. Re-search on blockchain and how it is used within smart cities has grown significantly over the last years. Nowadays, research on blockchain in smart cities is a heterogeneous research field with contributions from many different disciplines. This paper therefore seeks to provide an analysis and overview of the emergent research field of the application of blockchain technology in smart cities. We do so by conducting a bibliometric analysis of research published between 2014 and 2021. As results, we give a thematic and bibliometric overview of this research field. This includes an overview of the past research on blockchain for smart cities, its key topics, and main areas of research. Furthermore, by giving an overview of potential future research questions, we aim to provide researchers with a platform for further developments within this field.
Structure, Linear and Nonlinear Optical Properties of Different Dye-Modified TiO2 Ceramics for Possible Application in Photovoltaics
Authors: Barik R. , Panigrahi Manas R. , Sahoo A. K.
Published: December 2023

TiO2 is a highly potential, photoactive and dye-sensitive photocatalyst but its optical properties are further enhanced in the presence of different types of dyes. Dye-modified TiO2 was prepared by the modified sol–gel method. The dyes are methyl orange (MO), potassium permanganate (KMnO4) and beetroot. Analysis was conducted to study the physical, linear and nonlinear optical properties. XRD result suggests that the prepared TiO2 is synthesized in the pure anatase phase. The bandgap is decreased in all the dye-modified TiO2 samples. The dielectric constant, interband transition strength, energy loss functions, Urbach energy, optical conductivity and electrical conductivity were determined. Further, the dispersion parameter of refractive index was discussed as per the single-oscillator model proposed by Wemple and DiDomenico. Moreover, the analyses of nonlinear dispersions like first-order and third-order optical susceptibilities, electrical susceptibilities, nonlinear refractive index and reflection loss were performed. To further investigate the electronic properties, the plasma energy, Penn gap and Fermi energy were also determined.
A modified mouse model of Friedreich’s ataxia with conditional Fxn allele homozygosity delays onset of cardiomyopathy
Authors: Perfitt Tyler L. , Huichalaf Claudia , Gooch Renea , Kuperman Anna , Ahn Youngwook , Chen Xian , Ullas Soumya , Hirenallur-Shanthappa Dinesh , Zhan Yutian , Otis Diana , Whiteley Laurence O. , Bulawa Christine , Martelli Alain
Published: December 2023

Previous cardiac-specific frataxin knockout models exhibit rapid and fatal cardiomyopathy by 9 wk of age. This severe phenotype poses challenges for the design and execution of intervention studies. We introduce an alternative cardiac-specific model, MCK-Fxnflox/flox, with increased longevity and delayed onset of all major phenotypes. These phenotypes develop to the same severity as previous models. Thus, this new model provides the same cardiomyopathy-associated mortality with a larger window for potential studies.
Optimization of a cardiomyocyte model illuminates role of increased INa,L in repolarization reserve
Authors: Fullerton Kristin E. , Clark Alexander P. , Krogh-Madsen Trine , Christini David J.
Published: December 2023

Genetic algorithms are typically used to fit models or extract desired parameters from data. Here, we use the tool to produce a ventricular cardiomyocyte model with increased repolarization reserve. Since arrhythmia mitigation is dependent on multiple cardiac ion-channel conductances, study using a comprehensive, unbiased, and systems-level approach is important. The use of this optimization strategy allowed us to find robust profiles that illuminated unexpected mechanistic determinants of key ion-channel conductances in repolarization reserve.
Antifungal Activity of Bulgarian Rose Damascena Oil against Vaginitis‐Causing Opportunistic Fungi
Authors: Lee Yejin , Park Eunhye , Jang Bohee , Hwang Jisun , Lee Jinmin , Oh Eok-Soo
Published: December 2023

Since Bulgarian rose damascena oil is known for its anti‐inflammatory, antioxidant, and antimicrobial properties, we investigated its antifungal activity against the species of Candida, which are among the most common opportunistic fungal pathogens. Our disk‐diffusion assay revealed that Bulgarian rose damascena oil effectively inhibited the growth of Candida albicans along with various bacteria. The minimum inhibitory and fungicidal concentrations against Candida albicans and Candida glabrata were all 0.25%. Under our experimental conditions, Bulgarian rose damascena oil showed better inhibitory effects on Candida glabrata and Candida albicans than several popular essential oils reported to have antifungal activity other than Origanum vulgare oil. Interestingly, Bulgarian rose damascena oil showed better antifungal activity against Candida species at acidic pH and induced cell death of Candida species in the culture medium, with cell death seen in 25–35% of the cells exposed to 0.05% Bulgarian rose damascena oil. Furthermore, Bulgarian rose damascena oil inhibited the hyphal growth of Candida albicans cultured in the RPMI medium with fetal bovine serum. These findings collectively suggest that Bulgarian rose damascena oil has antifungal activity against Candida species and thus could potentially be developed in novel therapies for vaginitis‐causing pathogenic fungi.
Integration of Highly Graphitic Three-Dimensionally Ordered Macroporous Carbon Microspheres with Hollow Metal Oxide Nanospheres for Ultrafast and Durable Lithium-Ion Storage
Authors: Yang Soo Young , Park Jin-Sung , Koo Hye Young , Kang Yun Chan
Published: December 2023

Achieving excellent electrochemical performance at high charging rate has been a long-cherished dream in the field of lithium-ion batteries (LIBs). As a part of the efforts to meet the goal, an innovative strategy for the synthesis of 3D porous highly graphitic carbon microspheres, to which numerous hollow metal oxide nanospheres are anchored, for use as anode in LIBs is introduced. Hollow carbon nanosphere-aggregated microspheres prepared from the spray drying process are graphitized with the aid of metal catalysts, and subsequent oxidation selectively removed amorphous carbon, leading to the formation of highly conductive graphitic carbon matrix. Numerous hollow metal oxide nanospheres formed simultaneously during the oxidation process via nanoscale Kirkendall diffusion are anchored onto the carbonaceous matrix, effectively reinforcing the structural integrity by alleviating volume changes and reducing lithium-ion diffusion lengths. The synergistic effect of combining hollow metal oxide nanospheres with high theoretical capacity with conductive carbon matrix led to accelerated electrochemical kinetics, resulting in high capacity at high charging rate. In addition, trapping the hollow metal oxide nanospheres inside hollow carbon nanospheres could effectively alleviate the volume changes, which led to high structural stability. When applied as LIB anodes, the microspheres exhibit a capacity of 411 mA h g−1 after 2500 cycles at 10.0 A g−1, with ~80% capacity retention. The anode exhibits a high capacity of 274 mA h g−1 at an extremely high current density of 50.0 A g−1, thus demonstrating the structural merits of the microspheres.
Supplementary Figures from DPP9 Stabilizes NRF2 to Suppress Ferroptosis and Induce Sorafenib Resistance in Clear Cell Renal Cell Carcinoma
Authors: Chang Kun , Chen Yingji , Zhang Xuanzhi , Zhang Wei , Xu Ning , Zeng Bohan , Wang Yue , Feng Tao , Dai Bo , Xu Fujiang , Ye Dingwei , Wang Chenji
Published: December 2023

Supplementary Figures
Supplementary Figure S5 Part 2 from CK1δ and CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma
Authors: Burger Karen L. , Fernandez Mario R. , Meads Mark B. , Sudalagunta Praneeth , Oliveira Paula S. , Renatino Canevarolo Rafael , Alugubelli Raghunandan Reddy , Tungsevik Alexandre , De Avila Gabe , Silva Maria , Graeter Allison I. , Dai Hongyue A. , Vincelette Nicole D. , Prabhu Antony , Magaletti Dario , Yang Chunying , Li Weimin , Kulkarni Amit , Hampton Oliver , Koomen John M. , Roush William R. , Monastyrskyi Andrii , Berglund Anders E. , Silva Ariosto S. , Cleveland John L. , Shain Kenneth H.
Published: December 2023

Figure S5 Part 2
Supplementary Figure Legends from CK1δ and CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma
Authors: Burger Karen L. , Fernandez Mario R. , Meads Mark B. , Sudalagunta Praneeth , Oliveira Paula S. , Renatino Canevarolo Rafael , Alugubelli Raghunandan Reddy , Tungsevik Alexandre , De Avila Gabe , Silva Maria , Graeter Allison I. , Dai Hongyue A. , Vincelette Nicole D. , Prabhu Antony , Magaletti Dario , Yang Chunying , Li Weimin , Kulkarni Amit , Hampton Oliver , Koomen John M. , Roush William R. , Monastyrskyi Andrii , Berglund Anders E. , Silva Ariosto S. , Cleveland John L. , Shain Kenneth H.
Published: December 2023

Figure legends for the supplementary figures
Supplementary Data Title Page from CK1δ and CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma
Authors: Burger Karen L. , Fernandez Mario R. , Meads Mark B. , Sudalagunta Praneeth , Oliveira Paula S. , Renatino Canevarolo Rafael , Alugubelli Raghunandan Reddy , Tungsevik Alexandre , De Avila Gabe , Silva Maria , Graeter Allison I. , Dai Hongyue A. , Vincelette Nicole D. , Prabhu Antony , Magaletti Dario , Yang Chunying , Li Weimin , Kulkarni Amit , Hampton Oliver , Koomen John M. , Roush William R. , Monastyrskyi Andrii , Berglund Anders E. , Silva Ariosto S. , Cleveland John L. , Shain Kenneth H.
Published: December 2023

Title Page for Supplementary Data
Supplementary Data 3 from Neurotransmitter Receptor HTR2B Regulates Lipid Metabolism to Inhibit Ferroptosis in Gastric Cancer
Authors: Tu Ru-Hong , Wu Sheng-Ze , Huang Ze-Ning , Zhong Qing , Ye Yin-Hua , Zheng Chao-Hui , Xie Jian-Wei , Wang Jia-Bin , Lin Jian-Xian , Chen Qi-Yue , Huang Chang-Ming , Lin Mi , Lu Jun , Cao Long-Long , Li Ping
Published: December 2023

DEGs in sc-RNA seq between tumor cells with and without HTR2B expression
Supplementary Data 2 from Neurotransmitter Receptor HTR2B Regulates Lipid Metabolism to Inhibit Ferroptosis in Gastric Cancer
Authors: Tu Ru-Hong , Wu Sheng-Ze , Huang Ze-Ning , Zhong Qing , Ye Yin-Hua , Zheng Chao-Hui , Xie Jian-Wei , Wang Jia-Bin , Lin Jian-Xian , Chen Qi-Yue , Huang Chang-Ming , Lin Mi , Lu Jun , Cao Long-Long , Li Ping
Published: December 2023

DEGs in whole transcriptome sequencing between HGC-27 cells treated with 5-HT and control.
Figure S7 Part 1 from CK1δ and CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma
Authors: Burger Karen L. , Fernandez Mario R. , Meads Mark B. , Sudalagunta Praneeth , Oliveira Paula S. , Renatino Canevarolo Rafael , Alugubelli Raghunandan Reddy , Tungsevik Alexandre , De Avila Gabe , Silva Maria , Graeter Allison I. , Dai Hongyue A. , Vincelette Nicole D. , Prabhu Antony , Magaletti Dario , Yang Chunying , Li Weimin , Kulkarni Amit , Hampton Oliver , Koomen John M. , Roush William R. , Monastyrskyi Andrii , Berglund Anders E. , Silva Ariosto S. , Cleveland John L. , Shain Kenneth H.
Published: December 2023

Supplementary Figure S7 Part 1
Figure S9 from Neurotransmitter Receptor HTR2B Regulates Lipid Metabolism to Inhibit Ferroptosis in Gastric Cancer
Authors: Tu Ru-Hong , Wu Sheng-Ze , Huang Ze-Ning , Zhong Qing , Ye Yin-Hua , Zheng Chao-Hui , Xie Jian-Wei , Wang Jia-Bin , Lin Jian-Xian , Chen Qi-Yue , Huang Chang-Ming , Lin Mi , Lu Jun , Cao Long-Long , Li Ping
Published: December 2023

Supplementary Figure 9. HTR2B overexpression correlates with poor prognosis. (A) Kaplan–Meier survival analysis comparing disease-specific survival rates based on low vs. high expression of HTR2B mRNA in TCGA cohorts (n = 370). (B) Forest plot showing the hazard ratio for 5-year overall survival (OS) in our local dataset. (C) Kaplan–Meier survival analysis comparing overall survival in patients stratified by high versus low IHC scores for HTR2B, further classified by TNM stage of gastric cancer in the local cohort (n = 188). (D) Representative IHC staining of HIF1α in human gastric tumors. (E and F) Kaplan–Meier survival analysis comparing overall survival (E) and disease-specific survival (F) rates based on low vs. high expression of HIF1α mRNA in TCGA cohorts (n = 370).
Figure S2 from CK1δ and CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma
Authors: Burger Karen L. , Fernandez Mario R. , Meads Mark B. , Sudalagunta Praneeth , Oliveira Paula S. , Renatino Canevarolo Rafael , Alugubelli Raghunandan Reddy , Tungsevik Alexandre , De Avila Gabe , Silva Maria , Graeter Allison I. , Dai Hongyue A. , Vincelette Nicole D. , Prabhu Antony , Magaletti Dario , Yang Chunying , Li Weimin , Kulkarni Amit , Hampton Oliver , Koomen John M. , Roush William R. , Monastyrskyi Andrii , Berglund Anders E. , Silva Ariosto S. , Cleveland John L. , Shain Kenneth H.
Published: December 2023

Supplementary Figure S2
Supplementary Table S9 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S9. Explanation of clusters included in this analysis.
Supplementary Table S7 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S7. Summary of per cluster information across the full hierarchy built by RACCOON.
Supplementary Table S5 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S5. Antibodies used for immunohistochemistry.
Supplementary Table S4 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S4. Secondary antibodies used for immunofluorescence staining.
Supplementary Table S3 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S3. Primary antibodies used for immunofluorescence staining.
Supplementary Table S2 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S2. Codon-optimized sequence of NUTM1.
Supplementary Table S13 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Table S13. OTTER scores for mNC cell lines, including all cluster with score > 0.25.
Supplementary Fig. S6 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Fig. S6. Gross photos of stomach and esophagus obtained at necropsy of GEMM NC mice treated as indicated,
corresponding with Fig. 4H.
Supplementary Fig. S3 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Fig. S3. mNC-derived cell lines share transcriptional profiles with
tumor tissues that are characterized by MYC pathway upregulation.
Supplementary Fig. S2 from The BRD4–NUT Fusion Alone Drives Malignant Transformation of NUT Carcinoma
Authors: Durall R. Taylor , Huang Julianna , Wojenski Luke , Huang Yeying , Gokhale Prafulla C. , Leeper Brittaney A. , Nash Joshua O. , Ballester Pedro L. , Davidson Scott , Shlien Adam , Sotirakis Emmanuel , Bertaux Fabien , Dubus Vincent , Luo Jia , Wu Catherine J. , Keskin Derin B. , Eagen Kyle P. , Shapiro Geoffrey I. , French Christopher A.
Published: December 2023

Supplementary Fig. S2. mNC tumors arise from esophagus.
Supplementary Table S9 from EZH2 Cooperates with BRD4-NUT to Drive NUT Carcinoma Growth by Silencing Key Tumor Suppressor Genes
Authors: Huang Yeying , Durall R. Taylor , Luong Nhi M. , Hertzler Hans J. , Huang Julianna , Gokhale Prafulla C. , Leeper Brittaney A. , Persky Nicole S. , Root David E. , Anekal Praju V. , Montero Llopis Paula D.L.M. , David Clement N. , Kutok Jeffery L. , Raimondi Alejandra , Saluja Karan , Luo Jia , Zahnow Cynthia A. , Adane Biniam , Stegmaier Kimberly , Hawkins Catherine E. , Ponne Christopher , Le Quan , Shapiro Geoffrey I. , Lemieux Madeleine E. , Eagen Kyle P. , French Christopher A.
Published: December 2023

Supplementary Table S9
Supplementary Table S4 from EZH2 Cooperates with BRD4-NUT to Drive NUT Carcinoma Growth by Silencing Key Tumor Suppressor Genes
Authors: Huang Yeying , Durall R. Taylor , Luong Nhi M. , Hertzler Hans J. , Huang Julianna , Gokhale Prafulla C. , Leeper Brittaney A. , Persky Nicole S. , Root David E. , Anekal Praju V. , Montero Llopis Paula D.L.M. , David Clement N. , Kutok Jeffery L. , Raimondi Alejandra , Saluja Karan , Luo Jia , Zahnow Cynthia A. , Adane Biniam , Stegmaier Kimberly , Hawkins Catherine E. , Ponne Christopher , Le Quan , Shapiro Geoffrey I. , Lemieux Madeleine E. , Eagen Kyle P. , French Christopher A.
Published: December 2023

Supplementary Table S4
Supplementary Fig. S5 from EZH2 Cooperates with BRD4-NUT to Drive NUT Carcinoma Growth by Silencing Key Tumor Suppressor Genes
Authors: Huang Yeying , Durall R. Taylor , Luong Nhi M. , Hertzler Hans J. , Huang Julianna , Gokhale Prafulla C. , Leeper Brittaney A. , Persky Nicole S. , Root David E. , Anekal Praju V. , Montero Llopis Paula D.L.M. , David Clement N. , Kutok Jeffery L. , Raimondi Alejandra , Saluja Karan , Luo Jia , Zahnow Cynthia A. , Adane Biniam , Stegmaier Kimberly , Hawkins Catherine E. , Ponne Christopher , Le Quan , Shapiro Geoffrey I. , Lemieux Madeleine E. , Eagen Kyle P. , French Christopher A.
Published: December 2023

Supplementary Fig. S5
Supplementary Fig. S2 from EZH2 Cooperates with BRD4-NUT to Drive NUT Carcinoma Growth by Silencing Key Tumor Suppressor Genes
Authors: Huang Yeying , Durall R. Taylor , Luong Nhi M. , Hertzler Hans J. , Huang Julianna , Gokhale Prafulla C. , Leeper Brittaney A. , Persky Nicole S. , Root David E. , Anekal Praju V. , Montero Llopis Paula D.L.M. , David Clement N. , Kutok Jeffery L. , Raimondi Alejandra , Saluja Karan , Luo Jia , Zahnow Cynthia A. , Adane Biniam , Stegmaier Kimberly , Hawkins Catherine E. , Ponne Christopher , Le Quan , Shapiro Geoffrey I. , Lemieux Madeleine E. , Eagen Kyle P. , French Christopher A.
Published: December 2023

Supplementary Fig. S2
Supplementary Fig. S1 from EZH2 Cooperates with BRD4-NUT to Drive NUT Carcinoma Growth by Silencing Key Tumor Suppressor Genes
Authors: Huang Yeying , Durall R. Taylor , Luong Nhi M. , Hertzler Hans J. , Huang Julianna , Gokhale Prafulla C. , Leeper Brittaney A. , Persky Nicole S. , Root David E. , Anekal Praju V. , Montero Llopis Paula D.L.M. , David Clement N. , Kutok Jeffery L. , Raimondi Alejandra , Saluja Karan , Luo Jia , Zahnow Cynthia A. , Adane Biniam , Stegmaier Kimberly , Hawkins Catherine E. , Ponne Christopher , Le Quan , Shapiro Geoffrey I. , Lemieux Madeleine E. , Eagen Kyle P. , French Christopher A.
Published: December 2023

Supplementary Fig. S1
Figure 3 from The Next-Generation Oral Selective Estrogen Receptor Degrader Camizestrant (AZD9833) Suppresses ER+ Breast Cancer Growth and Overcomes Endocrine and CDK4/6 Inhibitor Resistance
Authors: Lawson Mandy , Cureton Natalie , Ros Susana , Cheraghchi-Bashi Azadeh , Urosevic Jelena , D'Arcy Sophie , Delpuech Oona , DuPont Michelle , Fisher David I. , Gangl Eric T. , Lewis Hilary , Trueman Dawn , Wali Neha , Williamson Stuart C. , Moss Jennifer , Montaudon Elodie , Derrien Heloise , Marangoni Elisabetta , Miragaia Ricardo J. , Gagrica Sladjana , Morentin-Gutierrez Pablo , Moss Thomas A. , Maglennon Gareth , Sutton Daniel , Polanski Radoslaw , Rosen Alan , Cairns Jonathan , Zhang Pei , Sánchez-Guixé Mònica , Serra Violeta , Critchlow Susan E. , Scott James S. , Lindemann Justin P.O. , Barry Simon T. , Klinowska Teresa , Morrow Christopher J. , S Carnevalli Larissa
Published: December 2023

Camizestrant has superior in vivo activity to fulvestrant in ESR1wt and ESR1m PDX models (1). A, Waterfall plot representing the growth of 28 of ER+ breast cancer PDX treated with camizestrant 10 mg/kg daily; bars are colored according to ESR1 mutational status and other genomic characteristics annotated at the top. The percentage change calculated from the initial volume at day of treatment is shown. Data represent mean ± SE of the mean. B, Waterfall plot representing the growth of 25 PDX treated with fulvestrant 5 mg/kg weekly. N/A denotes models where the head-to-head arm was not available. The percentage of change calculated from the initial volume at day of treatment is shown. Data represent mean ± SE of the mean. C, Pie chart indicates the proportions of PDX models sensitive or insensitive to camizestrant and/or fulvestrant. The antitumor response of camizestrant versus fulvestrant monotherapy is represented as the percentage of tumor change compared with the initial tumor volume, benchmarked to vehicle changes. D, Correlation of fulvestrant PDX antitumor response (y-axis) versus camizestrant (x-axis) in 25 PDX, represented as the percentage of tumor volume change compared with the initial tumor volume. Data represent mean. The boxes indicate the percentage change from baseline ≤100%; the percentage of models sensitive only to fulvestrant or camizestrant, or sensitive to both is represented in each box.
Data from Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression
Authors: Zhou Lijie , Luo Yongbo , Liu Yuenan , Zeng Youmiao , Tong Junwei , Li Mengting , Hou Yaxin , Du Kaixuan , Qi Yabin , Pan Wenbang , Liu Yuanhao , Wang Rongli , Tian Fengyan , Gu Chaohui , Chen Ke
Published: December 2023

Abstract
Fatty acid metabolism reprogramming is a prominent feature of clear cell renal cell carcinoma (ccRCC). Increased lipid storage supports ccRCC progression, highlighting the importance of understanding the molecular mechanisms driving altered fatty acid synthesis in tumors. Here, we identified that malonyl-CoA decarboxylase (MLYCD), a key regulator of fatty acid anabolism, was downregulated in ccRCC, and low expression correlated with poor prognosis in patients. Restoring MLYCD expression in ccRCC cells decreased the content of malonyl CoA, which blocked de novo fatty acid synthesis and promoted fatty acid translocation into mitochondria for oxidation. Inhibition of lipid droplet accumulation induced by MLYCD-mediated fatty acid oxidation disrupted endoplasmic reticulum and mitochondrial homeostasis, increased reactive oxygen species levels, and induced ferroptosis. Moreover, overexpressing MLYCD reduced tumor growth and reversed resistance to sunitinib in vitro and in vivo. Mechanistically, HIF2α inhibited MLYCD translation by upregulating expression of eIF4G3 microexons. Together, this study demonstrates that fatty acid catabolism mediated by MLYCD disrupts lipid homeostasis to repress ccRCC progression. Activating MLYCD-mediated fatty acid metabolism could be a promising therapeutic strategy for treating ccRCC.
Significance:
MLYCD deficiency facilitates fatty acid synthesis and lipid droplet accumulation to drive progression of renal cell carcinoma, indicating inducing MYLCD as a potential approach to reprogram fatty acid metabolism in kidney cancer.
Supplemental Table 1 from Unraveling Racial Disparities in Supportive Care Medication Use among End-of-Life Pancreatic Cancer Patients: Focus on Pain Management and Psychiatric Therapies
Authors: Allen John M. , Awunti MegCholack , Guo Yi , Bian Jiang , Rogers Sherise C. , Scarton Lisa , DeRemer David L. , Wilkie Diana J.
Published: December 2023

Supplemental Table 1 describes the specific Supportive Care Medications by Drug Class that were included in the study
Supplementary Materials from Prediction of Risk of Metastatic Recurrence for Female Breast Cancer Patients in the Presence of Competing Causes of Death
Authors: Mariotto Angela B. , Botta Laura , Bernasconi Alice , Zou Zhaohui , Gatta Gemma , Capocaccia Riccardo
Published: December 2023

The Supplementary Materials present details of the methods including notation and derivations of the paper equations.
Table S9 from Cancer Prevalence Projections in Japan and Decomposition Analysis of Changes in Cancer Burden, 2020–2050: A Statistical Modeling Study
Authors: Nguyen Phuong The , Hori Megumi , Matsuda Tomohiro , Katanoda Kota
Published: December 2023

Site-specific projections of 10-year prevalence count in 2020 and 2050, with decomposition of changes over 2020-2050
Table S7 from Cancer Prevalence Projections in Japan and Decomposition Analysis of Changes in Cancer Burden, 2020–2050: A Statistical Modeling Study
Authors: Nguyen Phuong The , Hori Megumi , Matsuda Tomohiro , Katanoda Kota
Published: December 2023

All-site combined projections of 10-year prevalence count and rate in 2020, 2030, 2040, and 2050
Table S3 from Cancer Prevalence Projections in Japan and Decomposition Analysis of Changes in Cancer Burden, 2020–2050: A Statistical Modeling Study
Authors: Nguyen Phuong The , Hori Megumi , Matsuda Tomohiro , Katanoda Kota
Published: December 2023

Projection scenarios
Table S2 from Cancer Prevalence Projections in Japan and Decomposition Analysis of Changes in Cancer Burden, 2020–2050: A Statistical Modeling Study
Authors: Nguyen Phuong The , Hori Megumi , Matsuda Tomohiro , Katanoda Kota
Published: December 2023

Sex- and site-specific model validation