bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolvement of Spatio-Temporal Pattern and Driving Forces Analysis of Ancient Trees Based on the Geographically Weighted Regression Model in Guangzhou and Foshan, China
Authors: Xu Zhenzhou , Xu Qing , Liu Kaiyan , Liu Yan , Du Jiaheng , Yi Kexin , Zhou Xiaokang , Lin Wei , Li Hui
Published: August 2024

Ancient trees play an important ecosystem service role in high-density cities, revealing the zonal distribution characteristics of vegetation under climate influence. The ancient trees in Guangzhou and Foshan in 2018 and 2023 were taken as study objects to explore the evolution of their spatio-temporal patterns and to analyze the spatial differentiation characteristics of their driving factors using the geographical weighted regression (GWR) model. The results showed the following: (1) The ancient trees in Guangzhou and Foshan were composed of typical subtropical evergreen broad-leaved forest communities, mainly represented by broad-leaved species of evergreen dicotyledonous plants. The dominant species mainly included Litchi chinensis, Ficus microcarpa, Canarium pimela, Ficus virens, and Dimocarpus longan. However, there was a significant difference between Guangzhou and Foshan. (2) The number of ancient trees in Guangzhou showed negative growth, while Foshan saw a significant increase. However, species diversity in both areas increased, with the highest diversity in the northeast, higher diversity in the south-central part, and lower diversity in the western and northwestern parts. (3) The maximum kernel density of ancient trees in Guangzhou and Foshan differed 22-fold, indicating a spatial distribution pattern of multiple clusters. (4) The GWR model effectively explained the driving factors of the heterogeneity of the spatial distribution of ancient trees. The results showed that artificial disturbance was the most important factor affecting the spatial distribution of ancient trees in high-density urban agglomerations in the same vegetation zone. The study clarified the characteristics of the spatial distribution and species diversity of ancient trees in the region, revealed the driving factors for the evolution of the spatial pattern of ancient trees in highly urbanized areas, and provided guidelines for policies and measures for enhancing biodiversity and conserving germplasm resources in the region.
Effect of Corn Straw Treated with Lactobacillus plantarum and Cellulase on Ruminal Fermentation and Microbiota of Hu Sheep
Authors: Wang Lijun , Wang Jiadai , Wang Ping , Liu Chaoqi , Li Xinxin , Chang Juan , Jin Sanjun , Yin Qingqiang , Zhu Qun
Published: August 2024

This study aimed to assess how the bacterial–enzyme co-fermentation of corn straw affects fermentation quality, the digestion rate in Hu sheep, and rumen microorganisms. Orthogonal experiments were utilized to establish the optimal fermentation conditions, which were subsequently applied in bacterial–enzyme fermentation trials involving four groups: group A (control), group B (enzyme added), group C (bacteria added), and group D (bacteria + enzyme). The results show that the optimal fermentation conditions were the addition of 2% corn flour, 2% cottonseed meal, and 60% moisture. In comparison with group A, group D demonstrated the most favorable outcomes, notably reducing the pH and cellulose content while enhancing the lactic acid content. The relative abundances of Pantoea and Weissella reduced, whereas those of Lactiplantibacillus and Limosilactobacillus increased, in the fermented corn straw. In the animal experiments, group D exhibited significantly higher digestibility of NDF and ADF; total VFA, acetic acid, and NH3-N contents; and enzyme activity compared with groups A and B. Additionally, the relative abundances of Prevotella, NK4A214-group, Entodinium, and Polyplastron increased, while those of Dasytricha and Enoploplastron decreased, in group D compared with group A. It can be concluded that Lactobacillus plantarum and cellulase treatments enhance the nutritional value of corn straw by improving ruminal fermentation and regulating the sheep rumen microbiota.
Unveiling the Acoustic Signature of Collichthys lucidus: Insights from X-ray Morphometry-Informed Acoustic Modeling and Empirical Analysis
Authors: Lyu Shuo , Qiu Chuhan , Xue Minghua , Zhu Zhenhong , Qiu Yue , Tong Jianfeng
Published: August 2024

Collichthys lucidus is an important small-scale economic fish species in the Yangtze River Estuary. To improve the accuracy of acoustic stock assessments for C. lucidus, it is necessary to accurately measure its target strength (TS). This study obtained precise morphological parameters of C. lucidus through X-ray scanning and established a Kirchhoff ray mode (KRM) model to simulate the changes in TS of the fish body and swimbladder at different acoustic frequencies and pitch angles. At the same time, the TS was measured using the tethered method to analyze and compare the broadband scattering characteristics obtained from both methods. An empirical formula of C. lucidus relating TS to body length at two conventional frequencies was established using the least squares method. The results show that the C. lucidus TS changes, with body length ranging from 10.91 to 16.61 cm, are significantly influenced by the pitch angle at 70 kHz and 200 kHz frequencies, and the fluctuation of TS for both the fish body and swimbladder increases with the rise in frequency. The broadband TS values estimated by the KRM model and measured by the tethered method fluctuate within in the ranges from −45 dB to −55 dB and −40 dB to −55 dB, respectively. The TS of C. lucidus tends to increase with the increase in swimbladder length. When the probability density function of the pitch angle is N(−5°, 15°), the b20 measured by the KRM and the tethered method at 70 kHz are −71.94 dB and −69.21 dB, respectively, while at 200 kHz they are −72.58 dB and −70.55 dB. This study provides a scientific basis for future acoustic target discrimination and stock assessment of C. lucidus in the Yangtze River Estuary.
Antimicrobial Resistance in Aquaculture: Risk Mitigation within the One Health Context
Authors: Milijasevic Milan , Veskovic-Moracanin Slavica , Babic Milijasevic Jelena , Petrovic Jelena , Nastasijevic Ivan
Published: August 2024

The application of antimicrobials in aquaculture primarily aims to prevent and treat bacterial infections in fish, but their inappropriate use may result in the emergence of zoonotic antibiotic-resistant bacteria and the subsequent transmission of resistant strains to humans via food consumption. The aquatic environment serves as a potential reservoir for resistant bacteria, providing an ideal breeding ground for development of antimicrobial resistance (AMR). The mutual inter-connection of intensive fish-farming systems with terrestrial environments, the food processing industry and human population creates pathways for the transmission of resistant bacteria, exacerbating the problem further. The aim of this study was to provide an overview of the most effective and available risk mitigation strategies to tackle AMR in aquaculture, based on the One Health (OH) concept. The stringent antimicrobial use guidelines, promoting disease control methods like enhanced farm biosecurity measures and vaccinations, alternatives to antibiotics (ABs) (prebiotics, probiotics, immunostimulants, essential oils (EOs), peptides and phage therapy), feeding practices, genetics, monitoring water quality, and improving wastewater treatment, rather than applying excessive use of antimicrobials, can effectively prevent the development of AMR and release of resistant bacteria into the environment and food. The contribution of the environment to AMR development traditionally receives less attention, and, therefore, environmental aspects should be included more prominently in OH efforts to predict, detect and prevent the risks to health. This is of particular importance for low and middle-income countries with a lack of integration of the national AMR action plans (NAPs) with the aquaculture-producing environment. Integrated control of AMR in fisheries based on the OH approach can contribute to substantial decrease in resistance, and such is the case in Asia, where in aquaculture, the percentage of antimicrobial compounds with resistance exceeding 50% (P50) decreased from 52% to 22% within the period of the previous two decades.
Management of Patients Receiving Anticoagulation Therapy in Dental Practice: A Systematic Review
Authors: Inchingolo Francesco , Inchingolo Angelo Michele , Piras Fabio , Ferrante Laura , Mancini Antonio , Palermo Andrea , Inchingolo Alessio Danilo , Dipalma Gianna
Published: August 2024

Background: Anticoagulant drugs are a valuable tool for minimizing thrombotic risks in at-risk patients. The purpose of this study is to conduct a literature review highlighting the management of these drugs during daily clinical dental practice. Materials and Methods: We limited our search to English-language papers published between 1 January 1989, and 7 March 2024, in PubMed, Scopus and Web of Science that were relevant to our topic. In the search approach, the Boolean keywords “anticoagulant AND dentistry” were used. Results: Twenty-five clinical trials were included for final review from 623 articles obtained from the databases Web of Science (83), PubMed (382), and Scopus (158), eliminating duplicates and 79 off-topic items, resulting in 419 articles after removing 315 entries and confirming eligibility. Overall, these studies support the use of local hemostatic measures to manage the risk of bleeding in patients on anticoagulant therapy undergoing dental procedures and highlight the importance of greater education and collaboration among healthcare professionals. Conclusions: Research and clinical investigation have improved understanding and management of dental procedures in patients undergoing anticoagulant or antiplatelet therapy. Hemostatic agents, clinical protocols, risk factors, and continuous education are essential for navigating the complexities of anticoagulant therapy, ensuring optimal outcomes and enhancing patient well-being.
Are Cytological and Morphological Analyses Sufficient in Ploidy Determination of Watermelon Haploid Plants?
Authors: Kara Ecem , Taşkın Hatıra , Karabıyık Şenay , Solmaz İlknur , Sarı Nebahat , Karaköy Tolga , Baktemur Gökhan
Published: August 2024

Watermelon (Citrullus lanatus L. Thunb. Matsum. and Nakai) is a vegetable species with high water content that is rich in lycopene and refreshing; therefore, breeding studies are carried out intensively to develop new varieties. The breeding period in these plants can be shortened with the use of the haploidization technique, and determining the ploidy levels of plants in haploidization studies is very important. In this study, it was examined whether morphological and cytological analyses would be sufficient for ploidy determination of haploid plants obtained by ovary culture in watermelon. With this aim, the stomatal, morphological and cytological characteristics were determined, taken from 15 haploid and 19 double haploid plants. The ploidy level of the plants was detected by flow cytometry before these analyses. In this study, “Principal Component Analysis” was performed based on average values to analyze the structure of the relationship between the parameters examined. It is thought that stomatal features can be used as morphological markers in determining the ploidy levels of plants. The differences obtained from the study results may help to develop effective strategies in determining the ploidy levels of plants.
Dormancy Release and Seed Germination in Tulipa saxatilis (Liliaceae) Coupled with Effects of Fertilization Schemes for Bulblet Development from Seedlings
Authors: Kozoni Marianthi , Samartza Ioulietta , Pipinis Elias , Kostas Stefanos , Anestis Ioannis , Karapatzak Eleftherios , Bareka Pepy , Hatzilazarou Stefanos , Tsoktouridis Georgios , Krigas Nikos
Published: August 2024

Tulips are among the most famous plants that are used mainly as ornamental cut flowers. Although widely cultivated, they are mainly reproduced asexually, and knowledge of their sexual reproduction is imperfect, especially in regard to botanical tulips. The aim of the present study was to investigate the temperature effect on seed germination of the Near Threatened wild-growing Greek subendemic plant Tulipa saxatilis (Liliaceae) and to define its seed dormancy type. Seed germination was facilitated by bioclimatic profiling generated with R software (version 4.3.3) connecting its natural distribution points with open access precipitation and temperature data, and it was assessed upon incubation in controlled growth chambers at five constant temperatures (5, 10, 15, 20 and 25 °C) under a 12:12 light–dark photoperiod. The seeds were dependent on temperature for germination and germinated only within a very narrow range of relatively low temperatures (5–15 °C) and optimally at 10 °C (93%), corresponding to natural winter temperatures and precipitation profiles. Increase in the incubation temperature at 20 and 25 °C resulted in no germination. The treatment of seeds with gibberellic acid (GA3) at two different concentrations (500, 1000 mg·L−1) did not widen the temperature range for seed germination. The seeds of T. saxatilis initially presented an underdeveloped embryo, and significant embryo development was detected only at low temperatures (10 and 15 °C) with almost triple embryo length after one month, thus confirming the existence of complex morphophysiological seed dormancy. Moreover, germinated seeds (with and without GA3) were planted in small-sized pots under greenhouse conditions, and the growth of bulblets was investigated in response to the application of commercially available chemical fertilizers, an integrated nutrient management scheme, and a biostimulant. The fertilization schemes affected the weight and length of the produced bulblets, whereas initial seed treatment with GA3 negatively affected the growth (weight, length, and width) of the produced bulblets.
Quantitative Proteomic Analysis Reveals Key Proteins Involved in Testicular Development of Yaks
Authors: La Yongfu , Ma Xiaoming , Bao Pengjia , Chu Min , Yan Ping , Guo Xian , Liang Chunnian
Published: August 2024

Male reproductive health is largely determined already in the early development of the testis. Although much work has been carried out to study the mechanisms of testicular development and spermatogenesis, there was previously no information on the differences in the protein composition of yak testicles during early development. In this study, the protein profiles in the testicles of 6- (M6), 18- (M18), and 30-month-old (M30) yaks were comparatively analyzed using TMT proteomics. A total of 5521 proteins were identified, with 13, 1295, and 1397 differentially expressed proteins (DEPs) in 30- vs. 18-, 18- vs. 6-, and 30- vs. 6-month-old testes, respectively. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEPs were mainly involved in signaling pathways related to testicular development and spermatogenesis, including the MAPK, PI3K–Akt, Wnt, mTOR, TGF-β, and AMPK signaling pathways. Furthermore, we also identified eight potential proteins (TEX101, PDCL2, SYCP2, SYCP3, COL1A1, COL1A2, ADAM10, and ATF1) that may be related to the testicular development and spermatogenesis of yaks. This study may provide new insights into the molecular mechanisms of the testicular development and spermatogenesis of yaks.
Genome-Wide Identification of Glutathione S-Transferase Family from Dendrobium officinale and the Functional Characterization of DoGST5 in Cadmium Tolerance
Authors: Jiang Wu , Wang Tao , Zhang Man , Duan Xiaojing , Chen Jiadong , Liu Yingying , Tao Zhengming , Guo Qiaosheng
Published: August 2024

Glutathione S-transferases (GSTs) are members of a protein superfamily with diverse physiological functions, including cellular detoxification and protection against oxidative damage. However, there is limited research on GSTs responding to cadmium (Cd) stress. This study classified 46 GST genes in Dendrobium officinale (D. officinale) into nine groups using model construction and domain annotation. Evolutionary analysis revealed nine subfamilies with diverse physical and chemical properties. Prediction of subcellular localization revealed that half of the GST members were located in the cytoplasm. According to the expression analysis of GST family genes responding to Cd stress, DoGST5 responded significantly to Cd stress. Transient expression of DoGST5-GFP in tobacco leaves revealed that DoGST5 was localized in the cytoplasm. DoGST5 overexpression in Arabidopsis enhanced Cd tolerance by reducing Cd-induced H2O2 and O2− levels. These findings demonstrate that DoGST5 plays a critical role in enhancing Cd tolerance by balancing reactive oxygen species (ROS) levels, offering potential applications for improving plant adaptability to heavy metal stress.
Comprehensive Transcriptome Analysis Expands lncRNA Functional Profiles in Breast Cancer
Authors: Zhu Wenyong , Huang Hao , Hu Zixi , Gu Yu , Zhang Rongxin , Shu Huiling , Liu Hongjia , Sun Xiao
Published: August 2024

Breast cancer is a heterogeneous disease that arises as a multi-stage process involving multiple cell types. Patients diagnosed with the same clinical stage and pathological classification may have different prognoses and therapeutic responses due to alterations in molecular genetics. As an essential marker for the molecular subtyping of breast cancer, long non-coding RNAs (lncRNAs) play a crucial role in gene expression regulation, cell differentiation, and the maintenance of genomic stability. Here, we developed a modular framework for lncRNA identification and applied it to a breast cancer cohort to identify novel lncRNAs not previously annotated. To investigate the potential biological function, regulatory mechanisms, and clinical relevance of the novel lncRNAs, we elucidated the genomic and chromatin features of these lncRNAs, along with the associated protein-coding genes and putative enhancers involved in the breast cancer regulatory networks. Furthermore, we uncovered that the expression patterns of novel and annotated lncRNAs identified in breast cancer were related to the hormone response in the PAM50 subtyping criterion, as well as the immune response and progression states of breast cancer across different immune cells and immune checkpoint genes. Collectively, the comprehensive identification and functional analysis of lncRNAs revealed that these lncRNAs play an essential role in breast cancer by altering gene expression and participating in the regulatory networks, contributing to a better insight into breast cancer heterogeneity and potential avenues for therapeutic intervention.
New Aspects Regarding the Fluorescence Spectra of Melanin and Neuromelanin in Pigmented Human Tissue Concerning Hypoxia
Authors: Leupold Dieter , Buder Susanne , Pfeifer Lutz , Szyc Lukasz , Riederer Peter , Strobel Sabrina , Monoranu Camelia-Maria
Published: August 2024

Melanin is a crucial pigment in melanomagenesis. Its fluorescence in human tissue is exceedingly weak but can be detected through advanced laser spectroscopy techniques. The spectral profile of melanin fluorescence distinctively varies among melanocytes, nevomelanocytes, and melanoma cells, with melanoma cells exhibiting a notably “red” fluorescence spectrum. This characteristic enables the diagnosis of melanoma both in vivo and in histological samples. Neuromelanin, a brain pigment akin to melanin, shares similar fluorescence properties. Its fluorescence can also be quantified with high spectral resolution using the same laser spectroscopic methods. Documented fluorescence spectra of neuromelanin in histological samples from the substantia nigra substantiate these findings. Our research reveals that the spectral behavior of neuromelanin fluorescence mirrors that of melanin in melanomas. This indicates that the typical red fluorescence is likely influenced by the microenvironment around (neuro)melanin, rather than by direct pigment interactions. Our ongoing studies aim to further explore this distinctive “red” fluorescence. We have observed this red fluorescence spectrum in post-mortem measurements of melanin in benign nevus. The characteristic red spectrum is also evident here (unlike the benign nevus in vivo), suggesting that hypoxia may contribute to this phenomenon. Given the central role of hypoxia in both melanoma development and treatment, as well as in fundamental Parkinson’s disease mechanisms, this study discusses strategies aimed at reinforcing the hypothesis that red fluorescence from (neuro)melanin serves as an indicator of hypoxia.
Singlet-Oxygen-Mediated Regulation of Photosynthesis-Specific Genes: A Role for Reactive Electrophiles in Signal Transduction
Authors: Pancheri Tina , Baur Theresa , Roach Thomas
Published: August 2024

During photosynthesis, reactive oxygen species (ROS) are formed, including hydrogen peroxide (H2O2) and singlet oxygen (1O2), which have putative roles in signalling, but their involvement in photosynthetic acclimation is unclear. Due to extreme reactivity and a short lifetime, 1O2 signalling occurs via its reaction products, such as oxidised poly-unsaturated fatty acids in thylakoid membranes. The resulting lipid peroxides decay to various aldehydes and reactive electrophile species (RES). Here, we investigated the role of ROS in the signal transduction of high light (HL), focusing on GreenCut2 genes unique to photosynthetic organisms. Using RNA seq. data, the transcriptional responses of Chlamydomonas reinhardtii to 2 h HL were compared with responses under low light to exogenous RES (acrolein; 4-hydroxynonenal), β-cyclocitral, a β-carotene oxidation product, as well as Rose Bengal, a 1O2-producing photosensitiser, and H2O2. HL induced significant (p < 0.05) up- and down-regulation of 108 and 23 GreenCut2 genes, respectively. Of all HL up-regulated genes, over half were also up-regulated by RES, including RBCS1 (ribulose bisphosphate carboxylase small subunit), NPQ-related PSBS1 and LHCSR1. Furthermore, 96% of the genes down-regulated by HL were also down-regulated by 1O2 or RES, including CAO1 (chlorophyllide-a oxygnease), MDH2 (NADP-malate dehydrogenase) and PGM4 (phosphoglycerate mutase) for glycolysis. In comparison, only 0–4% of HL-affected GreenCut2 genes were similarly affected by H2O2 or β-cyclocitral. Overall, 1O2 plays a significant role in signalling during the initial acclimation of C. reinhardtii to HL by up-regulating photo-protection and carbon assimilation and down-regulating specific primary metabolic pathways. Our data support that this pathway involves RES.
Effect of Salt Variability on the Low-Temperature Metal-Catalyzed Graphitization of PAN/DMSO Solutions for the Synthesis of Nanostructured Graphitic Carbon
Authors: Kim Taewoo , Kim Byoung-Suhk , Ko Tae , Kim Hak
Published: August 2024

Graphitic carbon plays a pivotal role in numerous technological applications, including energy storage, energy conversion, and different fields of material science. The transformation of amorphous carbon into graphitic carbon, a process known as graphitization, is important for optimizing the properties of carbon materials. In this study, we explore the catalytic graphitization of polyacrylonitrile (PANs) using various metal salts (LiNO3, Ca(NO3)2·4H2O, and Ni(NO3)2·6H2O). We prepared dimethyl sulfoxide (DMSO) solutions of PAN with different salt concentrations of 5, 10, and 15 wt.%. The different prepared metal salt-mixed PAN/DMSO solutions were dried at 45 °C and this was followed by carbonization processes at 950 °C, with a heating rate of 1 °C min−1 for 1 h under an N2 atmosphere. The resulting graphitic carbon was characterized to determine the influence of salt type and concentration on the degree of graphitization. Our findings provide valuable insights into PAN-derived graphitic carbon’s structural and compositional properties. This work underscores the influence of salt concentration in optimizing the graphitization process, offering a pathway to design facile and cost-effective graphitic carbon materials.
Experimental and Simulation Studies on Protective Structures in Floating Dock
Authors: Wang Zhengyao , Liu Kun , Liu Jingqiao , Meng Qingao , Qiu Weijian , Zong Shuai
Published: August 2024

In this research, two distinct designs of protective structures were developed to address structural damage caused by ships impacting the internal structures of floating docks during maintenance operations. The designed protective structures consist of support sections and load-bearing sections, with the load-bearing section comprising three frame sections. For ease of description, the front frame section, middle frame section, and rear frame section are referred to as Frame A, Frame B, and Frame C, respectively. A drop-weight test was conducted with a stern-shaped indenter impacting the structures at 3.89 m/s. This study also assessed varying impact speeds and positions. The results showed that Specimen 2 had localized indentations on Frame B, while Specimen 1 exhibited overall deformation of Frame B and additional deformations in Frame A. The simulations agreed with the experimental results, confirming the model’s accuracy. At speeds from 2.34 m/s to 5.45 m/s, Specimen 2 consistently showed localized deformations, while Specimen 1 showed comprehensive deformation of Frame B at 3.89 m/s due to lower rigidity. When the indenter impacted the specimens at different locations with a speed of 5.45 m/s, the two specimens exhibited varying degrees of damage. As the impact location shifted from the central area to the end, the maximum indentation depth of Specimen 1 decreased from 52.26 mm to 41.71 mm, while that of Specimen 2 decreased from 43.26 mm to 38.50 mm. The reduction in indentation depth and extent as the impact location approached the support frame can be attributed to the increasing involvement of the web plate beneath the frame in resisting the impact. Additionally, compared to Specimen 1, Specimen 2 exhibited a relatively smaller overall indentation depth, and the impact of location variation on indentation depth was also relatively minor.
Phylogenetic Insights Reveal New Taxa in Thyridariaceae and Massarinaceae
Authors: Tian Wen-Hui , Jin Yan , Liao Yue-Chi , Faraj Turki KH. , Guo Xin-Yong , Maharachchikumbura Sajeewa S. N.
Published: August 2024

Pleosporales is a highly diverse (and the largest) order in Dothideomycetes, and it is widespread in decaying plants in various environments around the world. During a survey of fungal diversity in Sichuan Province, China, specimens of hyphomycetous and Thyridaria-like fungi were collected from dead branches of pine trees and cherry trees. These taxa were initially identified as members of Massarinaceae and Thyridariaceae through morphological examination. Phylogenetic analyses of the Thyridariaceae, combining ITS, SSU, LSU, RPB2, and TEF1 sequence data, indicated a distinct clade sister to Pseudothyridariella and Thyridariella, distinct from any genus in the family. Thus, a new genus, Vaginospora, is proposed to accommodate the type species Vaginospora sichuanensis, which is characterized by semi-immersed globose to oblong ascomata with an ostiolar neck, cylindrical to clavate asci with an ocular chamber, and hyaline to dark brown, fusiform, 3–5-transversely septate ascospores with an inconspicuous mucilaginous sheath. Based on the morphological comparisons and multi-locus phylogenetic analyses (ITS, SSU, LSU, RPB2, and TEF1) of the Massarinaceae, we have identified three collections belonging to the genus Helminthosporium, leading us to propose H. filamentosa sp. nov., H. pini sp. nov., and H. velutinum as a new host record. According to Phylogenetic analysis, H. pini formed an independent clade sister to H. austriacum and H. yunnanense, and H. filamentosa represents the closest sister clade to H. quercinum. Helminthosporium pini is distinct from H. austriacum by the shorter conidiophores and H. yunnanense by the longer and wider conidia. The H. filamentosa differs from H. quercinum in having longer conidiophores and smaller conidia. This study extends our understanding of diversity within Thyridariaceae and Helminthosporium. Our findings underscore the rich biodiversity and potential for discovering novel fungal taxa within these groups.
Location Optimization Strategies for Corn Production and Distribution towards Sustainable Green Supply Chain
Authors: Asha Labiba Noshin , Aragon Lucy G. , Dey Arup , Yodo Nita
Published: August 2024

Background: The corn supply chain is vital for food security and economic stability regionally and globally. This study integrates sustainable supply chain management with location optimization to address trade-offs from climate change, economic viability, and environmental impact while assuming the constant social obligation inherent in the supply chain structure. Methods: This study employs a mixed-integer programming (MIP) framework to optimize facility locations in North Dakota, including corn production zones as suppliers and ethanol plants as consumers. Primary objectives include cost minimization and greenhouse gas reduction, enabling the prioritization of economic or environmental goals as per organizational strategies and regulations. This approach ultimately maximizes resource utilization by ensuring efficient production and distribution practices. Results: The case study results highlight the optimal selection of 20 out of 30 corn production zones to meet statewide ethanol plant demand efficiently. Using compressed natural gas (CNG) instead of diesel could potentially save USD 2 million annually and cut carbon emissions by up to 1148 thousand tons per year, demonstrating meaningful progress toward economic and environmental sustainability within the supply network. Conclusions: The presented work offers a systematic methodology for designing sustainable supply chains for various agricultural products, aligning with the broader goal of promoting sustainability and resilience for efficient agricultural production and distribution systems.
Micro/Nanomechanical Characterization of ScAlMgO4 Single Crystal by Instrumented Indentation and Scratch Methods
Authors: Ni Zifeng , Yu Jie , Chen Guomei , Ji Mingjie , Qian Shanhua , Bian Da , Liu Ming
Published: August 2024

ScAlMgO4 (SCAM), which can be used as an epitaxial substrate material of GaN in power devices, faces the challenge of achieving a high-quality surface by ultra-precision polishing due to its brittle and easily cleaved characteristics, which are closely associated with its mechanical properties. The micromechanical properties of SCAM single crystals were evaluated by nanoindentation and microscratch tests using different indenters. The elastic modulus EIT and the indentation hardness HIT of SCAM obtained by nanoindentation were 226 GPa and 12.1 GPa, respectively. Leaf-shaped chips and the associated step-like planes of SCAM can be found in the severely damaged regime during scratching by Berkovich and Vickers indenters with sharp edges due to the intersection of intense radial and lateral cracks. The fracture toughness (Kc = 1.12 MPa·m1/2) of SCAM can be obtained by using a scratch-based methodology for a spherical indenter based on linear elastic fracture mechanics (LEFM) under an appropriate range of applied loads. An optimal expression for calculating the fracture toughness of easily cleaved materials, including SCAM, via the Vickers indenter-induced cracking method using a Berkovich indenter was recommended.
Specially Structured AgCuTi Foil Enables High-Strength and Defect-Free Brazing of Sapphire and Ti6Al4V Alloys: The Microstructure and Fracture Characteristics
Authors: Liu Shaohong , Liu Hairui , Zhou Limin , Cui Hao , Liu Manmen , Chen Li , Wen Ming , Dong Haigang , Liu Feng , Wang Wei , Li Song
Published: August 2024

A novel AgCuTi brazing foil with a unique microstructure was developed, which could achieve strong vacuum brazing of Ti6Al4V (TC4) and sapphire. The brazing foil was composed of Ag solid solution (Ag(s,s)), Cu solid solution (Cu(s,s)), and layered Ti-rich phases, and had a low liquidus temperature of 790 °C and a narrow melting range of 16 °C, facilitating the defect-free joining of TC4 and sapphire. The sapphire/TC4 joint fabricated by using this novel AgCuTi brazing foil exhibited an outstanding average shear strength of up to 132.2 MPa, which was the highest value ever reported. The sapphire/TC4 joint had a characteristic structure, featuring a brazing seam reinforced by TiCu particles and a thin Ti3(Cu,Al)3O reaction layer of about 1.3 μm. The fracture mechanism of the sapphire/TC4 joint was revealed. The crack originated at the brazing seam with TiCu particles, then propagated through the Ti3(Cu,Al)3O reaction layer, detached the reaction layer from the sapphire, and finally penetrated into the sapphire. This study offers valuable insights into the design of active brazing alloys and reliable metal–ceramic bonding.
Assessment of the Radioactivity, Metals Content and Mineralogy of Granodiorite from Calabria, Southern Italy: A Case Study
Authors: Dattola Luigi , Belvedere Alberto , D’Agostino Maurizio , Faggio Giuliana , Majolino Domenico , Marguccio Santina , Messina Giacomo , Messina Maurizio , Mottese Antonio Francesco , Paladini Giuseppe , Venuti Valentina , Caridi Francesco
Published: August 2024

In this paper, an assessment of the natural radioactivity level, radon exhalation, metal contamination, and mineralogy of a granodiorite rock sample from Stilo, in the Calabria region, Southern Italy is presented as a case study. This rock was employed as a building material in the area under study. The specific activity of 226Ra, 232Th and 40K natural radioisotopes was assessed through high-purity germanium (HPGe) gamma-ray spectrometry. Then, several indices such as the absorbed gamma dose rate (D), the annual effective dose equivalent (AEDE), the activity concentration index (ACI) and the alpha index (Iα), were quantified to determine any potential radiological health risk related to radiation exposure from the analyzed rock. Furthermore, E-PERM electret ion chambers and inductively coupled plasma mass spectrometry (ICP-MS) measurements were carried out to properly quantify the radon exhalation rate and any possible metal pollution, respectively. In particular, to further address metal pollution factors, the geo-accumulation index (Igeo) was calculated to properly address the toxicity levels of the ecosystem originating from the detected metals. Finally, with the aim of successfully discriminating the provenance of such naturally occurring radionuclides, a combined approach involving X-ray diffraction (XRD) and µ-Raman spectroscopy was employed for the identification of the main radioisotope-bearing minerals characterizing the investigated granodiorite. The results achieved in this case study can be taken as the basis for further inquiries into background levels of radioactivity and chemical contamination in natural stone employed as building materials.
Concrete Aggregate-Gradation Effect and Strength-Criterion Modification for Fully Graded Hydraulic Concrete
Authors: Wang Chao , Qiu Qingming , Wang Xiaohua , Zhang Sherong , Wang Gaohui , Wei Peiyong
Published: August 2024

Utilization of large aggregates can promote energy conservation and emissions reductions, and large aggregates have been widely used in hydraulic concrete. The failure criterion for concrete material utilizing large aggregates forms the basis for constitutive models and structural design. However, the concrete failure criterion with respect to large aggregates has never been researched. To this end, the authors first conducted a series of triaxial compressive tests on concrete specimens with scaled aggregates. On this basis, several 3D mesoscopic numerical models were established with different aggregate gradations and used to simulate the triaxial compressive behaviors of hydraulic concrete after the models had been verified by experimental results. The results showed a pronounced aggregate-gradation effect on triaxial compressive behaviors, and concrete mixes with larger aggregates usually have higher compressive strength, especially under conditions of higher confinement. The normalized peak strength can increase by up to 23.49%. Finally, based on the available testing data, the strength criterion in different constitutive models is discussed and modified to allow more accurate simulation of the dynamic responses of and damage to fully graded concrete structures. This result can provide a theoretical basis on which construction entities can optimize the mix proportions of fully graded concrete and detect the failure modes of concrete structures.
Regulating TiO2 Deposition Using a Single-Anchored Ligand for High-Efficiency Perovskite Solar Cells
Authors: Xu Zhanpeng , Lan Zhineng , Chen Fuxin , Yin Chong , Wang Longze , Li Zhehan , Yan Luyao , Ji Jun
Published: August 2024

Planar perovskite solar cells (PSCs), as a promising photovoltaic technology, have been extensively studied, with strong expectations for commercialization. Improving the power conversion efficiency (PCE) of PSCs is necessary to accelerate their practical application, in which the electron transport layer (ETL) plays a key part. Herein, a single-anchored ligand of phenylphosphonic acid (PPA) is utilized to regulate the chemical bath deposition of a TiO2 ETL, further improving the PCE of planar PSCs. The PPA possesses a steric benzene ring and a phosphoric acid group, which can inhibit the particle aggregation of the TiO2 film through steric hindrance, leading to optimized interface (ETL/perovskite) contact. In addition, the incorporated PPA can induce the upshift of the Fermi-level of the TiO2 film, which is beneficial for interfacial electron transport. As a consequence, the PSCs with PPA-TiO2 achieve a PCE of 24.83%, which is higher than that (24.21%) of PSCs with TiO2. In addition, the unencapsulated PSCs with PPA-TiO2 also exhibit enhanced stability when stored in ambient conditions.
The Influence of Adding B4C and CeO2 on the Mechanical Properties of Laser Cladding Nickel-Based Coatings on the Surface of TC4 Titanium Alloy
Authors: Xu Shanna , Han Keqi , Wang Haili , Xi Yuntao , Wang Lei , Dong Xikai
Published: August 2024

The development of titanium alloys is limited by issues such as low hardness, poor wear resistance, and sensitivity to adhesive wear. Using laser cladding technology to create high-hardness wear-resistant coatings on the surface of titanium alloys is an economical and efficient method that can enhance their surface hardness and wear resistance. This paper presents the preparation of two types of nickel-based composite coatings, Ni60-Ti-Cu-xB4C and Ni60-Ti-Cu-B4C-xCeO2, on the surface of TC4 titanium alloy using laser cladding. When the B4C addition was 8 wt.%, the hardness of the cladding layer was the highest, with an average microhardness of 1078 HV, which was 3.37 times that of the TC4 substrate. The friction coefficient was reduced by 24.7% compared to the TC4 substrate, and the wear volume was only 2.7% of that of the substrate material. When the CeO2 content was 3 wt.%, the hardness of the cladding layer was the highest, with an average microhardness of 1105 HV, which was 3.45 times that of the TC4 substrate. The friction coefficient was reduced by 33.7% compared to the substrate material, and the wear volume was only 1.8% of that of the substrate material.
Characterisation of Large-Sized REBaCuO Bulks for Application in Flux Modulation Machines
Authors: Nouailhetas Quentin , Xing Yiteng , Dorget Rémi , Dirahoui Walid , Guijosa Santiago , Trillaud Frederic , Lévêque Jean , Noudem Jacques Guillaume , Labbé Julien , Berger Kévin
Published: August 2024

High temperature superconductors (HTSs) are enablers of extensive electrification for aircraft propulsion. Indeed, if used in electrical machines, HTS materials can drastically improve their performance in terms of the power-to-weight ratio. Among the different topologies of superconducting electrical machines, a flux modulation machine based on HTS bulks is of interest for its compactness and light weight. Such a machine is proposed in the FROST (Flux-barrier Rotating Superconducting Topology) project led by Airbus to develop new technologies as part of their decarbonization goals driven by international policies. The rotor of the machine will house large ring-segment-shaped HTS bulks in order to increase the output power. However, the properties of those bulks are scarcely known and have barely been investigated in the literature. In this context, the present work aims to fill out partially this scarcity within the framework of FROST. Thus, a thorough characterisation of the performances and homogeneity of 11 large REBaCuO bulks was carried out. Ten of the bulks are to be utilized in the machine prototype, originally keeping the eleventh bulk as a spare. A first set of characterisation was conducted on the eleven bulks. For this set, the trapped field mapping and the critical current were estimated. Then, a series of in-depth characterisations on the eleventh bulk followed. It included critical current measurement, X-ray diffraction, and scanning electron microscopy on different millimetre-size samples cut out from the bulk at various locations. The X-ray diffraction and scanning electron microscopy showed weakly oxygenated regions inside the bulk explaining the local drop or loss in superconducting properties. The objective was to determine the causes of the inhomogeneities found in the trapped field measured on all the bulks, sacrificing one of them, here the spare one. To help obtain a clearer picture, a numerical model was then elaborated to reproduce the field map of the eleventh bulk using the experimental data obtained from the characterisation of its various small samples. It is concluded that further characterisations, including the statistics on various bulks, are still needed to understand the underlying reasons for inhomogeneity in the trapped field. Nonetheless, all the bulks presented enough current density to be usable in the construction of the proposed machine.
Microstructure, Non-Basal Texture and Strength-Ductility of Extruded Mg–6Bi–3Zn Alloy
Authors: Li Xin , Mao Jian , Huang Xuefei , Huang Weigang
Published: August 2024

To investigate the influence of Zn-alloying on the microstructure and tensile mechanical properties of Mg–6Bi alloy after hot extrusion, a new ternary Mg–6Bi–3Zn alloy was prepared by extrusion at 300 °C. The microstructures, texture, dynamic precipitates and tensile mechanical behaviors of the extruded alloy were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), electron backscattered diffraction (EBSD) and a material testing machine at room temperature. After extrusion, the Mg–6Bi–3Zn alloy possesses a bimodal microstructure with elongated large unrecrystallized (unDRXed) grains and fine dynamic recrystallized (DRXed) grains. In addition, non-basal <202_1>//ED, <448_3>//ED and <112_1>//ED textures are observed within DRXed grains due to the Zn addition, leading to texture weakening in the extruded Mg–6Bi–3Zn alloy. Zn addition facilitates the dynamic precipitation behavior, leading to a 12.2% area fraction of Mg3Bi2 precipitates with an average size of 39.2 nm. Furthermore, incorporation of Zn atoms in Mg3Bi2 phases and segregation of Zn at the grain boundary are found. The extruded Mg–6Bi–3Zn alloy exhibits a tensile strength of 336 ± 7.1 MPa and a yield strength of 290 ± 5.5 MPa, as well as an elongation of 11.5%. Therefore, Zn addition is beneficial to enhance strength and keep good ductility for the extruded Mg–6Bi–3Zn alloy.
Experimental Investigations and Constitutive Modeling of the Dynamic Recrystallization Behavior of a Novel GH4720Li Superalloys with Yttrium Micro-Alloying
Authors: Yan Zehua , Hu Jiahui , Sun Shouxue
Published: August 2024

GH4720Li is an advanced nickel-based alloy celebrated for its remarkable high-temperature strength. This study aimed to investigate the dynamic recrystallization (DRX) behavior of novel GH4720Li superalloys microalloyed with 0.3Y via hot compression tests. A constitutive model was formulated to simulate the DRX behavior. Utilizing the stress–strain curve, the activation energy for the alloy was determined using both the Arrhenius model and the Z-parameter equation, resulting in 1117.916 kJ/mol. The microstructure evolution analysis conducted revealed that lower strain rates at elevated temperatures effectively hindered the occurrence of DRX. Conversely, the increase in the strain rate promoted DRX, producing uniform, equiaxial grains. Recrystallization calculations, along with validation experiments, demonstrated the efficacy of the Avrami model in establishing a DRX model for the alloy during hot deformation. This model accurately quantified DRX percentages under varying deformation parameters, showcasing strong agreement with the microstructure test results. The predictive capability afforded by the developed models offers valuable insights for optimizing the alloy’s forging process. During the compression of the novel GH4720Li superalloy, DRX initiates when the dislocation density in a specific region surpasses a critical threshold. Concurrently, dislocation accumulation near the grain boundaries exceeds that within the grains themselves, highlighting that newly formed DRXed grains primarily emerge along the deformed grain boundaries.
Surgical Drainage and Simultaneous Sinus Floor Augmentation in Patients with Chronic Maxillary Sinusitis
Authors: Park Won-Bae , Seol Hye-Kyoung , Shin Seungil , Hong Ji-Youn
Published: August 2024

Chronic maxillary sinusitis accompanied by severe thickening of the sinus mucosa, blockage of the ostium, and patient-reported symptoms requires preoperative assessment and treatment by an otolaryngologist before maxillary sinus floor augmentation (MSFA). Prescription of antibiotics and nasal saline irrigation are the first choice of treatment; however, endoscopic sinus surgery is considered when the treatment’s effect is limited and drug resistance is observed. Nevertheless, MSFA performed in the presence of sinus pathologies have been reported to have favorable results when the lesions are managed properly. This report presents cases of two patients who required MSFA but were diagnosed with chronic maxillary sinusitis (case 1 with nasal sinusitis and case 2 with dental sinusitis). After 2 weeks of antibiotic therapy, endoscopic surgery was recommended due to minimal changes in the size of the sinus lesion; however, the patients refused because of improved self-reported symptoms. Therefore, intraoral surgical drainage was planned as an alternative treatment. A large bony window was prepared at the lateral wall of the maxillary sinus, and a long intentional incision was made to improve access for the suction tip in various directions and depths into the sinus cavity. Thorough suction of the purulent exudate and saline irrigation were performed through this access. The size of the perforated area was reduced along with the elevation of the Schneiderian membrane from the sinus floor, and simultaneous bone grafting with implant placement was performed. Prosthesis was delivered after 6–8 months. At 1-year follow-up after loading, favorable outcomes of implant survival and maintenance of augmented bone height were observed, with no recurrence of postoperative sinusitis. Within the limitations of the present case report, thorough sinus drainage and saline irrigation during maxillary sinus floor augmentation resolved sinus infection in patients with chronic maxillary sinusitis with short-term clinical outcomes.