bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method
Authors: Takabatake Kiyofumi , Matsubara Masakazu , Yamachika Eiki , Fujita Yuki , Arimura Yuki , Nakatsuji Kazuki , Nakano Keisuke , Nagatsuka Histoshi , Iida Seiji
Published: November 2021

Background: We investigated and compared the osteogenic potential and bone regeneration capacities of dedifferentiated fat cells (DFAT cells) and adipose-derived stem cells (ASCs). Method: We isolated DFAT cells and ASCs from GFP mice. DFAT cells were established by a new culture method using a mesh culture instead of a ceiling culture. The isolated DFAT cells and ASCs were incubated in osteogenic medium, then alizarin red staining, alkaline phosphatase (ALP) assays, and RT-PCR (for RUNX2, osteopontin, DLX5, osterix, and osteocalcin) were performed to evaluate the osteoblastic differentiation ability of both cell types in vitro. In vivo, the DFAT cells and ASCs were incubated in osteogenic medium for four weeks and seeded on collagen composite scaffolds, then implanted subcutaneously into the backs of mice. We then performed hematoxylin and eosin staining and immunostaining for GFP and osteocalcin. Results: The alizarin red-stained areas in DFAT cells showed weak calcification ability at two weeks, but high calcification ability at three weeks, similar to ASCs. The ALP levels of ASCs increased earlier than in DFAT cells and showed a significant difference (p < 0.05) at 6 and 9 days. The ALP levels of DFATs were higher than those of ASCs after 12 days. The expression levels of osteoblast marker genes (osterix and osteocalcin) of DFAT cells and ASCs were higher after osteogenic differentiation culture. Conclusion: DFAT cells are easily isolated from a small amount of adipose tissue and are readily expanded with high purity; thus, DFAT cells are applicable to many tissue-engineering strategies and cell-based therapies.
Uric Acid in Inflammation and the Pathogenesis of Atherosclerosis
Authors: Kimura Yoshitaka , Tsukui Daisuke , Kono Hajime
Published: November 2021

Hyperuricemia is a common metabolic syndrome. Elevated uric acid levels are risk factors for gout, hypertension, and chronic kidney diseases. Furthermore, various epidemiological studies have also demonstrated an association between cardiovascular risks and hyperuricemia. In hyperuricemia, reactive oxygen species (ROS) are produced simultaneously with the formation of uric acid by xanthine oxidases. Intracellular uric acid has also been reported to promote the production of ROS. The ROS and the intracellular uric acid itself regulate several intracellular signaling pathways, and alterations in these pathways may result in the development of atherosclerotic lesions. In this review, we describe the effect of uric acid on various molecular signals and the potential mechanisms of atherosclerosis development in hyperuricemia. Furthermore, we discuss the efficacy of treatments for hyperuricemia to protect against the development of atherosclerosis.
A Comprehensive Analysis of Human Endogenous Retroviruses HERV-K (HML.2) from Teratocarcinoma Cell Lines and Detection of Viral Cargo in Microvesicles
Authors: Morozov Vladimir A. , Morozov Alexey V.
Published: November 2021

About 8% of our genome is composed of sequences from Human Endogenous Retroviruses (HERVs). The HERV-K (HML.2) family, here abbreviated HML.2, is able to produce virus particles that were detected in cell lines, malignant tumors and in autoimmune diseases. Parameters and properties of HML.2 released from teratocarcinoma cell lines GH and Tera-1 were investigated in detail. In most experiments, analyzed viruses were purified by density gradient centrifugation. HML.2 structural proteins, reverse transcriptase (RT) activity, viral RNA (vRNA) and particle morphology were analyzed. The HML.2 markers were predominantly detected in fractions with a buoyant density of 1.16 g/cm3. Deglycosylation of TM revealed truncated forms of transmembrane (TM) protein. Free virions and extracellular vesicles (presumably microvesicles—MVs) with HML.2 elements, including budding intermediates, were detected by electron microscopy. Viral elements and assembled virions captured and exported by MVs can boost specific immune responses and trigger immunomodulation in recipient cells. Sequencing of cDNA clones demonstrated exclusive presence of HERV-K108 env in HML.2 from Tera-1 cells. Not counting two recombinant variants, four known env sequences were found in HML.2 from GH cells. Obtained results shed light on parameters and morphology of HML.2. A possible mechanism of HML.2-induced diseases is discussed.
Nanoparticle-Mediated Delivery Systems in Photodynamic Therapy of Colorectal Cancer
Authors: Winifred Nompumelelo Simelane Nokuphila , Abrahamse Heidi
Published: November 2021

Colorectal cancer (CRC) involving a malignant tumour remains one of the greatest contributing causes of fatal mortality and has become the third globally ranked malignancy in terms of cancer-associated deaths. Conventional CRC treatment approaches such as surgery, radiation, and chemotherapy are the most utilized approaches to treat this disease. However, they are limited by low selectivity and systemic toxicity, so they cannot completely eradicate this disease. Photodynamic therapy (PDT) is an emerging therapeutic modality that exerts selective cytotoxicity to cancerous cells through the activation of photosensitizers (PSs) under light irradiation to produce cytotoxic reactive oxygen species (ROS), which then cause cancer cell death. Cumulative research findings have highlighted the significant role of traditional PDT in CRC treatment; however, the therapeutic efficacy of the classical PDT strategy is restricted due to skin photosensitivity, poor cancerous tissue specificity, and limited penetration of light. The application of nanoparticles in PDT can mitigate some of these shortcomings and enhance the targeting ability of PS in order to effectively use PDT against CRC as well as to reduce systemic side effects. Although 2D culture models are widely used in cancer research, they have some limitations. Therefore, 3D models in CRC PDT, particularly multicellular tumour spheroids (MCTS), have attracted researchers. This review summarizes several photosensitizers that are currently used in CRC PDT and gives an overview of recent advances in nanoparticle application for enhanced CRC PDT. In addition, the progress of 3D-model applications in CRC PDT is discussed.
Three-Dimensional Surface Crack Growth of Maraging Steel Spherical Pressure Shell
Authors: Zhu Yongmei , Li Rujun , Yang Jiahao
Published: November 2021

This study focused on the three-dimensional surface crack growth of a spherical pressure shell. Eight maraging steel 18Ni (250) samples were fabricated and tested, and the fatigue crack growth rate curves were obtained. Considering the influence of plastic closure effect and sample thickness on crack growth, the fitting formula of fatigue crack growth only related to materials was obtained. Based on the three-dimensional crack closure theory and the strip yield model, a three-dimensional surface crack growth model of spherical pressure shell was established. By using a self-written program and FRANC3D, the three-dimensional surface crack growth simulations of the spherical shell were completed. The influence of the initial shape ratio and initial depth of the crack on the crack growth and the fatigue life of the spherical shell was analyzed.
Push-Out Bond Strength of Resin-Modified Glass Ionomer Cement and Flowable Composite Luting Systems on Glass Fiber Post of Root Canal
Authors: Cheruvathoor Jalison Jacob , Thomas Lincy Rachel , Thomas Lirin Ann , Shivanna Madhuniranjanswamy Mahalakshmamma , Machani Pramod , Naik Sachin , Al Kheraif Abdulaziz Abdullah
Published: November 2021

Background: Posts that have been properly fitted can withstand torsion forces and so provide better retention. The push-out bonding strength of glass fiber posts to the root canal was evaluated using resin-modified glass ionomer cement (RMGIC) and flowable composite (FC). Method: Forty single-rooted maxillary central incisors were used in the study. The samples were randomly divided into two groups of 20 teeth each. The crown-down procedure was used to clean and shape the pulp area. A Tenax fiber trans Coltene whaletene post was used by both groups. The first group utilized FC (Filtek Z 350 3M ESPE) to coat the post, whereas the second group used RMGIC (Rely X 3M ESPE). The specimens were cross-sectioned after 24 h. Specimens were cross-sectioned four millimeters thick into coronal and middle parts using a sectioning machine, yielding 40 specimens per group. The strength of the bond between the luting cement and the posts was measured using push-out bond strength testing. We loaded the components at a cross speed of 0.5 mm/min on a universal testing machine until the bond failed. Results: The FC group had a 4.80 N push-out bond strength, whereas the RMGIC group had a 7.11 N push-out bond strength. Conclusion: FC’s mean push-out bond strength score is lower than RMGIC’s.
Optimal Synthesis and Application of a Si–Ti–Al Ternary Alloy as an Anode Material for Lithium-Ion Batteries
Authors: Lee Jaehan , Kim Young-Min , Kim Ju-Han , Jeong Jee-Woon , Lee Donghyun , Sung Jae Wook , Ahn Young Ju , Shim Jae-Hyun , Lee Sanghun
Published: November 2021

The development of novel anode materials for high energy density is required. Alloying Si with other metals is a promising approach to utilize the high capacity of Si. In this work, we optimized the composition of a Si–Ti–Al ternary alloy to achieve excellent electrochemical performance in terms of capacity, cyclability, and rate capability. The detailed internal structures of the alloys were characterized through their atomic compositions and diffraction patterns. The lithiation process of the alloy was monitored using real-time scanning electron microscopy, revealing that the mechanical stability of the optimized alloy was strongly enhanced compared to that of the pure silicon material.
Corrosion Resistance and Surface Bioactivity of Ti6Al4V Alloy after Finish Turning under Ecological Cutting Conditions
Authors: Leksycki Kamil , Kaczmarek-Pawelska Agnieszka , Ochał Kamil , Gradzik Andrzej , Pimenov Danil Yurievich , Giasin Khaled , Chuchala Daniel , Wojciechowski Szymon
Published: November 2021

The influence of cooling conditions and surface topography after finish turning of Ti6Al4V titanium alloy on corrosion resistance and surface bioactivity was analyzed. The samples were machined under dry and minimum quantity lubrication (MQL) conditions to obtain different surface roughness. The surface topographies of the processed samples were assessed and measured using an optical profilometer. The produced samples were subjected to electrochemical impedance spectroscopy (EIS) and corrosion potential tests (Ecorr) in the presence of simulated body fluid (SBF). The surface bioactivity of the samples was assessed on the basis of images from scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) analysis. The inspection of the surfaces of samples after turning under dry and MQL conditions revealed unevenly distributed precipitation of hydroxyapatite compounds (Ca/P) with a molar ratio in the range of 1.73–1.97. Regardless of the cutting conditions and surface roughness, the highest values of Ecorr ~0 mV were recorded on day 7 of immersion in the SBF solution. The impedance characteristics showed that, compared to the MQL conditions, surfaces machined under dry conditions were characterized by greater resistance and the presence of a passive layer on the processed surface. The main novelty of the paper is the study of the effect of ecological machining conditions, namely, dry and MQL cutting on the corrosion resistance and surface bioactivity of Ti6Al4V titanium alloy after finish turning. The obtained research results have practical significance. They can be used by engineers during the development of technological processes for medical devices made of Ti6Al4V alloy to obtain favorable functional properties of these devices.
ROS-Based Nanoparticles for Atherosclerosis Treatment
Authors: Hu Xin , Zhao Pengxuan , Lu Yongping , Liu Yani
Published: November 2021

Atherosclerosis (AS), a chronic arterial disease, is the leading cause of death in western developed countries. Considering its long-term asymptomatic progression and serious complications, the early prevention and effective treatment of AS are particularly important. The unique characteristics of nanoparticles (NPs) make them attractive in novel therapeutic and diagnostic applications, providing new options for the treatment of AS. With the assistance of reactive oxygen species (ROS)-based NPs, drugs can reach specific lesion areas, prolong the therapeutic effect, achieve targeted controlled release and reduce adverse side effects. In this article, we reviewed the mechanism of AS and the generation and removal strategy of ROS. We further discussed ROS-based NPs, and summarized their biomedical applications in scavenger and drug delivery. Furthermore, we highlighted the recent advances, challenges and future perspectives of ROS-based NPs for treating AS.
Fabrication of MNPs/rGO/PMMA Composite for the Removal of Hazardous Cr(VI) from Tannery Wastewater through Batch and Continuous Mode Adsorption
Authors: Ullah Rahman , Ahmad Waqas , Yaseen Muhammad , Khan Mansoor , Iqbal Khattak Mehmood , Mohamed Jan Badrul , Ikram Rabia , Kenanakis George
Published: November 2021

Herein, we report the synthesis of magnetic nanoparticle (MNP)-reduced graphene oxide (rGO) and polymethylmethacrylate (PMMA) composite (MNPs/rGO/PMMA) as adsorbent via an in situ fabrication strategy and, in turn, the application for adsorptive removal and recovery of Cr(VI) from tannery wastewater. The composite material was characterized via XRD, FTIR and SEM analyses. Under batch mode experiments, the composite achieved maximum adsorption of the Cr(VI) ion (99.53 ± 1.4%, i.e., 1636.49 mg of Cr(VI)/150 mg of adsorbent) at pH 2, adsorbent dose of 150 mg/10 mL of solution and 30 min of contact time. The adsorption process was endothermic, feasible and spontaneous and followed a pseudo-2nd order kinetic model. The Cr ions were completely desorbed (99.32 ± 2%) from the composite using 30 mL of NaOH solution (2M); hence, the composite exhibited high efficiency for five consecutive cycles without prominent loss in activity. The adsorbent was washed with distilled water and diluted HCl (0.1M), then dried under vacuum at 60 °C for reuse. The XRD analysis confirmed the synthesis and incorporation of magnetic iron oxide at 2θ of 30.38°, 35.5°, 43.22° and 57.36°, respectively, and graphene oxide (GO) at 25.5°. The FTIR analysids revealed that the composite retained the configurations of the individual components, whereas the SEM analysis indicated that the magnetic Fe3O4–NPs (MNPs) dispersed on the surface of the PMMA/rGO sheets. To anticipate the behavior of breakthrough, the Thomas and Yoon–Nelson models were applied to fixed-bed column data, which indicated good agreement with the experimental data. This study evaluates useful reference information for designing a cost-effective and easy-to-use adsorbent for the efficient removal of Cr(VI) from wastewater. Therefore, it can be envisioned as an alternative approach for a variety of unexplored industrial-level operations.
Complex Structural Effects in Deformed High-Manganese Steel
Authors: Kowalska Joanna , Ryś Janusz , Cempura Grzegorz
Published: November 2021

The research presented in this paper is part of a larger project concerning deformation behavior, microstructure and mechanical properties of high-manganese steels with different chemical compositions and processed under various conditions. The current investigation deals with the development of microstructure and crystallographic texture of Fe-21.2Mn-2.73Al-2.99Si steel deformed in tension until fracture at ambient temperature. The deformation process of the examined steel turned out to be complex and included not only dislocation slip and twinning but also strain induced phase transformations (γ → ε) and (γ → α′). The formation of ε-martensite with hexagonal structure was observed within the microstructure of the steel starting from the range of lower strains. With increasing deformation degree, the α′-martensite showing a cubic structure gradually began to form. Attempts have been made to explain the circumstances or conditions for the occurrence of the deformation mechanisms mentioned above and their impact on the mechanical properties. The obtained results indicate that the strength and plastic properties of the steel substantially exceed those of plain carbon steels. Since both, mechanical twinning and the strain-induced phase transformations took place during deformation, it seems that both types of deformation mechanisms contributed to an increase in the mechanical properties of the examined manganese steel.
Solid-State NMR and Raman Spectroscopic Investigation of Fluoride-Substituted Apatites Obtained in Various Thermal Conditions
Authors: Pajchel Lukasz , Borkowski Leszek
Published: November 2021

Fluoride-substituted apatites were synthesized by the standard sol-gel method and then calcined at three different temperatures: 800 °C, 1000 °C, and 1200 °C. Using a similar method, hydroxyapatite was synthesized and used as a reference material. The obtained powders were characterized by physicochemical methods: powder X-ray diffractometry, Raman spectroscopy, transmission electron microscopy, and solid-state nuclear magnetic resonance. All these methods allowed to identify additional α-TCP phase (tricalcium phosphate) in the HAP samples heated at 1000 °C and 1200 °C while fluoridated apatites turned out to be thermally stable. Moreover, Raman spectroscopy and NMR allowed to establish that the powders substituted with fluoride ions are not pure fluorapatite and contain OH- groups in the crystal structure. All the obtained materials had crystals with a shape similar to that of biological apatite.
A Facile Design of Colourimetric Polyurethane Nanofibrous Sensor Containing Natural Indicator Dye for Detecting Ammonia Vapour
Authors: Pakolpakçıl Ayben , Draczyński Zbigniew
Published: November 2021

Chemicals and industrial gases endanger both human health and the environment. The inhalation of colourless ammonia gas (NH3) can cause organ damage or even death in humans. Colourimetric materials are becoming more popular in the search for smart textiles for both fashion and specific occupational applications. Colourimetric textile sensors based on indicator dyes could be very useful for detecting strong gaseous conditions and monitoring gas leaks. In this study, black carrot extract (BCE) as a natural indicator dye and polyurethane (PU) polymer were used to develop a colourimetric sensor by electrospinning. The properties of the BCE/PU nanofibrous mats were characterized by the Fourier transform infrared spectrum (FTIR) and a scanning electron microscope (SEM). The BCE caused a change in the morphology of the PU nanofibrous mat. To evaluate the colour shift due to NH3 vapour, the BCE/PU nanofibrous mats were photographed by a camera, and software was used to obtain the quantitative colour data (CIE L*a*b). The BCE/PU nanofibrous exhibited a remarkable colour change from pink–red to green–blue under NH3 vapour conditions with a fast response time (≤30 s). These findings showed that colourimetric nanofibrous textile sensors could be a promising in situ material in protective clothing that changes colour when exposed to harmful gases.
Catalysts for the Conversion of CO2 to Low Molecular Weight Olefins—A Review
Authors: Pawelec Barbara , Guil-López Rut , Mota Noelia , Fierro Jose , Navarro Yerga Rufino
Published: November 2021

There is a large worldwide demand for light olefins (C2=–C4=), which are needed for the production of high value-added chemicals and plastics. Light olefins can be produced by petroleum processing, direct/indirect conversion of synthesis gas (CO + H2) and hydrogenation of CO2. Among these methods, catalytic hydrogenation of CO2 is the most recently studied because it could contribute to alleviating CO2 emissions into the atmosphere. However, due to thermodynamic reasons, the design of catalysts for the selective production of light olefins from CO2 presents different challenges. In this regard, the recent progress in the synthesis of nanomaterials with well-controlled morphologies and active phase dispersion has opened new perspectives for the production of light olefins. In this review, recent advances in catalyst design are presented, with emphasis on catalysts operating through the modified Fischer–Tropsch pathway. The advantages and disadvantages of olefin production from CO2 via CO or methanol-mediated reaction routes were analyzed, as well as the prospects for the design of a single catalyst for direct olefin production. Conclusions were drawn on the prospect of a new catalyst design for the production of light olefins from CO2.
Counterintuitive Single-Molecule Magnet Behaviour in Two Polymorphs of One-Dimensional Compounds Involving Chiral BINOL-Derived Bisphosphate Ligands
Authors: Mattei Carlo Andrea , Lefeuvre Bertrand , Dorcet Vincent , Argouarch Gilles , Cador Olivier , Lalli Claudia , Pointillart Fabrice
Published: November 2021

The coordination reaction of the [Dy(hfac)3(H2O)2] units (hfac− = 1,1,1,5,5,5-hexafluoroacetylacetonate) with the [8′-(Diphenoxylphosphinyl)[1,1′-binaphthalen]-8-yl]diphenoxylphosphine oxide ligand (L) followed by a crystallisation in a 1:3 CH2Cl2:n-hexane solvent mixture led to the isolation of a new polymorph of formula [(Dy(hfac)3((S)-L))3]n (1). The X-ray structure on single crystal of 1 revealed the formation of a mono-dimensional coordination polymer with three crystallographically independent DyIII centres, which crystallised in the polar chiral P21 space group. Ac magnetic measurements highlighted single-molecule magnet behaviour under both zero and 1000 Oe applied magnetic field with magnetic relaxation through quantum tunneling of the magnetisation (QTM, zero field only) and Raman processes. Despite the three crystallographically independent DyIII centres adopting a distorted D4d coordination environment, a single slow magnetic relaxation contribution was observed at a slower rate than its previously studied [(Dy(hfac)3((S)-L))]n (2) polymorph.
Magnetic Behaviour of Perovskite Compositions Derived from BiFeO3
Authors: Salak Andrei N. , Cardoso João Pedro V. , Vieira Joaquim M. , Shvartsman Vladimir V. , Khalyavin Dmitry D. , Fertman Elena L. , Fedorchenko Alexey V. , Pushkarev Anatoli V. , Radyush Yury V. , Olekhnovich Nikolai M. , Tarasenko Róbert , Feher Alexander , Čižmár Erik
Published: November 2021

The phase content and sequence, the crystal structure, and the magnetic properties of perovskite solid solutions of the (1−y)BiFeO3–yBiZn0.5Ti0.5O3 series (0.05 ≤ y ≤ 0.90) synthesized under high pressure have been studied. Two perovskite phases, namely the rhombohedral R3c and the tetragonal P4mm, which correspond to the structural types of the end members, BiFeO3 and BiZn0.5Ti0.5O3, respectively, were revealed in the as-synthesized samples. The rhombohedral and the tetragonal phases were found to coexist in the compositional range of 0.30 ≤ y ≤ 0.90. Magnetic properties of the BiFe1−y[Zn0.5Ti0.5]yO3 ceramics with y < 0.30 were measured as a function of temperature. The obtained compositional variations of the normalized unit-cell volume and the Néel temperature of the BiFe1−y[Zn0.5Ti0.5]yO3 perovskites in the range of their rhombohedral phase were compared with the respective dependences for the BiFe1−yB3+yO3 perovskites (where B3+ = Ga, Co, Mn, Cr, and Sc). The role of the high-pressure synthesis in the formation of the antiferromagnetic states different from the modulated cycloidal one characteristic of the parent BiFeO3 is discussed.
Indefinite Linear-Quadratic Stochastic Control Problem for Jump-Diffusion Models with Random Coefficients: A Completion of Squares Approach
Authors: Moon Jun , Chung Jin-Ho
Published: November 2021

In this paper, we study the indefinite linear-quadratic (LQ) stochastic optimal control problem for stochastic differential equations (SDEs) with jump diffusions and random coefficients driven by both the Brownian motion and the (compensated) Poisson process. In our problem setup, the coefficients in the SDE and the objective functional are allowed to be random, and the jump-diffusion part of the SDE depends on the state and control variables. Moreover, the cost parameters in the objective functional need not be (positive) definite matrices. Although the solution to this problem can also be obtained through the stochastic maximum principle or the dynamic programming principle, our approach is simple and direct. In particular, by using the Itô-Wentzell’s formula, together with the integro-type stochastic Riccati differential equation (ISRDE) and the backward SDE (BSDE) with jump diffusions, we obtain the equivalent objective functional that is quadratic in control u under the positive definiteness condition, where the approach is known as the completion of squares method. Then the explicit optimal solution, which is linear in state characterized by the ISRDE and the BSDE jump diffusions, and the associated optimal cost are derived by eliminating the quadratic term of u in the equivalent objective functional. We also verify the optimality of the proposed solution via the verification theorem, which requires solving the stochastic HJB equation, a class of stochastic partial differential equations with jump diffusions.
A Bayesian-Deep Learning Model for Estimating COVID-19 Evolution in Spain
Authors: Cabras Stefano
Published: November 2021

This work proposes a semi-parametric approach to estimate the evolution of COVID-19 (SARS-CoV-2) in Spain. Considering the sequences of 14-day cumulative incidence of all Spanish regions, it combines modern Deep Learning (DL) techniques for analyzing sequences with the usual Bayesian Poisson-Gamma model for counts. The DL model provides a suitable description of the observed time series of counts, but it cannot give a reliable uncertainty quantification. The role of expert elicitation of the expected number of counts and its reliability is DL predictions’ role in the proposed modelling approach. Finally, the posterior predictive distribution of counts is obtained in a standard Bayesian analysis using the well known Poisson-Gamma model. The model allows to predict the future evolution of the sequences on all regions or estimates the consequences of eventual scenarios.
Monte Carlo Algorithms for the Extracting of Electrical Capacitance
Authors: Kuznetsov Andrei , Sipin Alexander
Published: November 2021

We present new Monte Carlo algorithms for extracting mutual capacitances for a system of conductors embedded in inhomogeneous isotropic dielectrics. We represent capacitances as functionals of the solution of the external Dirichlet problem for the Laplace equation. Unbiased and low-biased estimators for the capacitances are constructed on the trajectories of the Random Walk on Spheres or the Random Walk on Hemispheres. The calculation results show that the accuracy of these new algorithms does not exceed the statistical error of estimators, which is easily determined in the course of calculations. The algorithms are based on mean value formulas for harmonic functions in different domains and do not involve a transition to a difference problem. Hence, they do not need a lot of storage space.
TopoResNet: A Hybrid Deep Learning Architecture and Its Application to Skin Lesion Classification
Authors: Hu Chuan-Shen , Lawson Austin , Chen Jung-Sheng , Chung Yu-Min , Smyth Clifford , Yang Shih-Min
Published: November 2021

The application of artificial intelligence (AI) to various medical subfields has been a popular topic of research in recent years. In particular, deep learning has been widely used and has proven effective in many cases. Topological data analysis (TDA)—a rising field at the intersection of mathematics, statistics, and computer science—offers new insights into data. In this work, we develop a novel deep learning architecture that we call TopoResNet that integrates topological information into the residual neural network architecture. To demonstrate TopoResNet, we apply it to a skin lesion classification problem. We find that TopoResNet improves the accuracy and the stability of the training process.
Hybrid Carbon Nanotube Flow near the Stagnation Region over a Permeable Vertical Plate with Heat Generation/Absorption
Authors: Anuar Nur Syazana , Bachok Norfifah , Pop Ioan
Published: November 2021

This research explored the mixed convection flow past a vertical plate immersed in a hybrid carbon nanotube near the stagnation point. The hybrid carbon nanotube was synthesized by the mixture of two nanoparticles, namely multi-wall (MWCNT) and single-wall (SWCNT) carbon nanotubes immersed in water (base fluid). In addition, attractive aspects of suction/injection and heat generation/absorption effects were incorporated. Similarity variables were used to convert the partial differential equations describing the fluid into ordinary (similarity) differential equations before being solved numerically using Matlab software. The simultaneous impact of several parameters on velocity and temperature profiles, skin friction coefficient, and local Nusselt number were represented with graphs. Dual solutions were observed for some pertinent parameters, which led to stability analysis. This analysis interpreted that merely the first numerical solution is stable. In addition, hybrid nanoparticle, injection effect, and heat-generation parameters led to a decreased range of solutions, whilst the suction effect and heat-absorption parameters acted in the opposite manner. Besides, it is noted that the rate of heat transfer for hybrid carbon nanotube was higher when compared with carbon nanotube and ordinary fluid. Additionally, the heat absorption and buoyancy-assisting flow parameters magnified the heat transfer rate.
Function of Follicular Cytokines: Roles Played during Maturation, Development and Implantation of Embryo
Authors: Adamczak Rafał , Ukleja-Sokołowska Natalia , Lis Kinga , Dubiel Mariusz
Published: November 2021

A balance within the immune system is necessary for the proper development of ovarian follicles. Numerous cytokines were detected in follicular fluid, the role of which in reproductive physiology seems crucial. They influence the development and maturation of the follicle, ovulation, and corpus luteum formation, as well as embryo implantation and maintenance of pregnancy. The analysis of follicular fluid requires its collection by puncturing of the ovary, which is usually executed in connection with various gynaecological procedures. When interpreting such test results, clinical indications for a given procedure and the method of patient preparation should be taken into account. This review revealed the results of currently available studies on the concentration of pro-inflammatory cytokines in follicular fluid in various forms of infertility. Additionally, it presented cytokines, whose concentration has a significant impact on the size of ovarian follicles, their number, the effectiveness of in vitro fertilisation, development of the embryo, and chances of correct implantation. Despite the many recent publications, the knowledge of follicular fluid immunology in the context of reproductive pathology is superficial and further research is required to extensively understand the roles of individual cytokines in reproductive pathology. In the future, this knowledge may enable patients’ individual qualifications to individual methods of infertility treatment, as well as the possible adjustment of the treatment regimen to the patient’s immune profile.
Clinical Outcomes of Chemotherapeutic Molecules as Single and Multiple Agents in Advanced Non-Small-Cell Lung Carcinoma (NSCLC) Patients
Authors: Kwan Ting Yoon , Chowdhury Ezharul Hoque
Published: November 2021

Background and Objectives: Lung cancer is the second most common cancer in the world. Non-small-cell lung carcinoma (NSCLC) makes up 85% of all lung cancer cases and the majority of patients are diagnosed when the cancer is advanced. Over the years, many anticancer drugs have been designed and introduced into the market to treat patients with advanced NSCLC. This review aims to discuss the comparative therapeutic benefits of conventional chemotherapeutics and other drugs available for treating advanced NSCLC. Materials and Methods: A literature search for first-line treatment of advanced NSCLC was carried out on PubMed and Google Scholar. Objective response rate (ORR) and overall survival were chosen as target endpoints. Results: Monotherapy showed lower treatment endpoints compared to combination therapy. Different combinations of platinum-based doublets demonstrated similar efficacies in treating NSCLC. However, pemetrexed–platinum doublets showed significantly better treatment endpoint in patients with non-squamous NSCLC. Most studies showing the best complete response rate (CRR) utilized epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKI), while most studies producing the best overall survival included programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) inhibitors in their treatment regimens. Conclusions: The findings of this review indicate that targeted therapy using specific inhibitors is now the most promising first-line anticancer treatment available in the market. However, chemotherapy is still effective in treating advanced NSCLC and is viable as a first-line treatment.
Efficacy and Safety of Sugammadex for the Reversal of Rocuronium-Induced Neuromuscular Blockade in Patients with End-Stage Renal Disease: A Systematic Review and Meta-Analysis
Authors: Kim Young-Sung , Lim Byung-Gun , Won Young-Ju , Oh Seok-Kyeong , Oh Jung-Suk , Cho Soo-Ah
Published: November 2021

Background and Objectives: Sugammadex is widely used in anesthesia to reverse rocuronium-induced neuromuscular blockade (NMB). In patients with compromised kidney function, most drugs show alteration of their pharmacokinetic profile with reduced clearance. The purpose of this article is to examine the efficacy, pharmacokinetics, and safety of sugammadex in end-stage renal disease (ESRD) patients receiving general anesthesia, using a systematic review. Materials and Methods: The databases of PubMed, EMBASE, the Cochrane Library, Web of Science, Scopus, KoreaMed, and ClinicalTrials.gov were searched for studies comparing the efficacy or safety outcomes of sugammadex administration for the reversal of rocuronium-induced NMB, in ESRD patients (group R) or in those with normal renal function (group N) undergoing surgery under general anesthesia. Results: We identified nine studies with 655 patients—six prospective, case-control studies with 179 patients (89 and 90 in groups R and N) and three retrospective observational studies with 476 ESRD patients. In the six prospective studies, the times taken to reach a train-of-four ratio ≥0.9, 0.8, and 0.7 were significantly longer in group R than in group N (weighted mean difference [95% confidence interval] [min]: 1.14 [0.29 to 2.00], 0.9 [0.24 to 1.57], 0.89 [0.20 to 1.57], respectively). The total plasma clearance of sugammadex was significantly lower in group R than in group N. There was no significant difference in the incidence of NMB recurrence and prolonged time to recovery between the groups. In the three retrospective studies, the possibility of sugammadex-related adverse events appears to be insignificant. Conclusions: Sugammadex may effectively and safely reverse rocuronium-induced NMB in patients with ESRD, although the recovery to a TOF ratio of 0.9 may be prolonged compared to patients with normal renal function. Further studies are needed, considering the small number of studies included and the high heterogeneity of some of the results.
Emphysema-Predominant COPD Had a Greater 5-Year Mortality and a Worse Annual Decline in Lung Function Than Airway Obstruction-Predominant COPD or Asthma at Initial Same Degree of Airflow Obstruction
Authors: Lin Chang-Wei , Huang Hung-Yu , Chung Fu-Tsai , Lo Chun-Yu , Huang Yu-Chen , Wang Ting-Wen , Yang Lan-Yan , Pan Yu-Bin , Chung Kian Fan , Wang Chun-Hua
Published: November 2021

Background and Objectives: We studied whether the extent of exertional oxygen desaturation and emphysema could cause greater mortality in COPD and asthma independent of airflow obstruction. Materials and Methods: We performed a 5-year longitudinal observational study in COPD and asthma patients who matched for airflow obstruction severity. All subjects performed a 6-min walk test (6MWT) and high-resolution computed tomography (HRCT) and followed spirometry and oxygen saturation (SpO2) during the 6MWT every 3–6 months. Overall survival was recorded. Cumulative survival curves were performed according to the Kaplan–Meier method and compared with the log-rank test. Results: The COPD group had higher emphysema scores, higher Δinspiratory capacities (ICs) and lower SpO2 during the 6MWT, which showed a greater yearly decline in FEV1 (40.6 mL) and forced vital capacity (FVC) (28 mL) than the asthma group (FEV1, 9.6 mL; FVC, 1.2 mL; p < 0.05). The emphysema-predominant COPD group had an accelerated annual decline in lung function and worse survival. The nadir SpO2 ≤ 80% and a higher emphysema score were the strong risk factors for mortality in COPD patients. Conclusions: The greater structural changes with a higher emphysema score and greater desaturation during the 6MWT in COPD may contribute to worse yearly decline in FEV1 and higher five-year mortality than in asthma patients with a similar airflow obstruction. The lowest SpO2 ≤ 80% during the 6MWT and emphysema-predominant COPD were the strong independent factors for mortality in chronic obstructive airway disease patients.
Impacts of Constitutive and Induced Benzoxazinoids Levels on Wheat Resistance to the Grain Aphid (Sitobion avenae)
Authors: Zhang Zhanfeng , Lan Hao , Cao Hehe , Hu Xiangshun , Fan Yongliang , Song Yue , Wu Lijuan , Liu Tong-Xian
Published: November 2021

Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and toxic effects against a wide range of herbivore pests. However, the relationship between benzoxazinoid level and plant resistance to aphids remains controversial. In this study, we investigated the relationship between benzoxazinoids composition and concentration in wheat leaves and the resistance to the grain aphid Sitobion avenae. Overall, six benzoxazinoids were detected and identified by mass spectrometry based metabolites profiling, including three lactams, two hydroxamic acids, and one methyl derivative. The constitutive levels of these benzoxazinoids were significantly different among the wheat varieties/lines. However, none of these benzoxazinoids exhibited considerable correlation with aphid resistance. S. avenae feeding elevated the level of 2-O-β-D-glucopyranosyloxy-4,7-dimethoxy-(2H)-1,4-benzoxazin-3(4H)-one (HDMBOA-Glc) and reduced the level of 2-O-β-D-glucopyranosyloxy-4-hydroxy-7-(2H)-methoxy-1,4-benzoxazin-3(4H)-one (DIMBOA-Glc) in some of the wheat varieties/lines. Moreover, aphid-induced level of DIMBOA-Glc was positively related with callose deposition, which was closely associated with aphid resistance. Wheat leaves infiltrated with DIMBOA-Glc caused a noticeable increase of callose deposition and the effect was in a dose dependent manner. This study suggests that the constitutive level of benzoxazinoids has limited impact on S. avenae. Aphid feeding can affect the balance of benzoxazinoids metabolism and the dynamic level of benzoxazinoids can act as a signal of callose deposition for S. avenae resistance. This study will extend our understanding of aphid–wheat interaction and provides new insights in aphid-resistance wheat breeding.
Preventing the Increase in Lysophosphatidic Acids: A New Therapeutic Target in Pulmonary Hypertension?
Authors: Duflot Thomas , Tu Ly , Leuillier Matthieu , Messaoudi Hind , Groussard Déborah , Feugray Guillaume , Azhar Saïda , Thuillet Raphaël , Bauer Fabrice , Humbert Marc , Richard Vincent , Guignabert Christophe , Bellien Jérémy
Published: November 2021

Cardiovascular diseases (CVD) are the leading cause of premature death and disability in humans that are closely related to lipid metabolism and signaling. This study aimed to assess whether circulating lysophospholipids (LPL), lysophosphatidic acids (LPA) and monoacylglycerols (MAG) may be considered as potential therapeutic targets in CVD. For this objective, plasma levels of 22 compounds (13 LPL, 6 LPA and 3 MAG) were monitored by liquid chromatography coupled with tandem mass spectrometry (HPLC/MS2) in different rat models of CVD, i.e., angiotensin-II-induced hypertension (HTN), ischemic chronic heart failure (CHF) and sugen/hypoxia(SuHx)-induced pulmonary hypertension (PH). On one hand, there were modest changes on the monitored compounds in HTN (LPA 16:0, 18:1 and 20:4, LPC 16:1) and CHF (LPA 16:0, LPC 18:1 and LPE 16:0 and 18:0) models compared to control rats but these changes were no longer significant after multiple testing corrections. On the other hand, PH was associated with important changes in plasma LPA with a significant increase in LPA 16:0, 18:1, 18:2, 20:4 and 22:6 species. A deleterious impact of LPA was confirmed on cultured human pulmonary smooth muscle cells (PA-SMCs) with an increase in their proliferation. Finally, plasma level of LPA(16:0) was positively associated with the increase in pulmonary artery systolic pressure in patients with cardiac dysfunction. This study demonstrates that circulating LPA may contribute to the pathophysiology of PH. Additional experiments are needed to assess whether the modulation of LPA signaling in PH may be of interest.
Hafnia alvei Pneumonia: A Rare Cause of Infection in a Patient with COVID-19
Authors: Méndez Lucía , Ferreira Jorge , Caneiras Cátia
Published: November 2021

Herein, we describe a case report of a critically ill patient, a 48-year-old man without comorbidities admitted to the hospital with a serious type 1 (hypoxemic) respiratory insufficiency and confirmed diagnosis of COVID-19. After 5 days with invasive mechanical ventilation, the patient developed a bacterial co-infection, namely a pneumonia by Hafnia alvei, requiring the last line of respiratory support: extracorporeal membrane oxygenation (ECMO). Subsequently, his clinical situation gradually stabilized, until he was discharged from the hospital on day 61, being accompanied in ambulatory consultation by the physical medicine and pulmonology department during the post-COVID-19 recovery. H. alvei is a Gram-negative bacterium that is rarely isolated from human specimens and is rarely considered to be pathogenic. However, COVID-19 disease can cause substantial organ dysfunction and can be associated with bacterial secondary infections which can favor the emergence of rare infectious diseases by uncommon microorganisms.
Characterization of Clinical Escherichia coli Strains Producing a Novel Shiga Toxin 2 Subtype in Sweden and Denmark
Authors: Bai Xiangning , Scheutz Flemming , Dahlgren Henrik Mellström , Hedenström Ingela , Jernberg Cecilia
Published: November 2021

Shiga toxin (Stx) is the key virulence factor in the Shiga Toxin-Producing Escherichia coli (STEC), which can cause diarrhea and hemorrhagic colitis with potential life-threatening complications. There are two major types of Stx: Stx1 and Stx2. Several Stx1/Stx2 subtypes have been identified in E. coli, varying in sequences, toxicity and host specificity. Here, we report a novel Stx2 subtype (designated Stx2m) from three clinical E. coli strains isolated from diarrheal patients and asymptomatic carriers in Sweden and Denmark. The Stx2m toxin was functional and exhibited cytotoxicity in vitro. The two Swedish Stx2m-producing strains belonged to the same serotype O148:H39 and Multilocus Sequencing Typing (MLST) Sequence Type (ST) 5825, while the Danish strain belonged to the O96:H19 serotype and ST99 type. Whole-genome sequencing (WGS) data analysis revealed that the three Stx2m-producing strains harbored additional virulence genes and the macrolide resistance gene mdf (A). Our findings expand the pool of Stx2 subtypes and highlight the clinical significance of emerging STEC variants. Given the clinical relevance of the Stx2m-producing strains, we propose to include Stx2m in epidemiological surveillance of STEC infections and clinical diagnosis.
ZrSnO4: A Solution-Processed Robust Electron Transport Layer of Efficient Planar-Heterojunction Perovskite Solar Cells
Authors: Choi Jun , Park Young Ki , Lee Hee Dong , Hong Seok Il , Lee Woosung , Jung Jae Woong
Published: November 2021

A robust electron transport layer (ETL) is an essential component in planar-heterojunction perovskite solar cells (PSCs). Herein, a sol-gel-driven ZrSnO4 thin film is synthesized and its optoelectronic properties are systematically investigated. The optimized processing conditions for sol-gel synthesis produce a ZrSnO4 thin film that exhibits high optical transmittance in the UV-Vis-NIR range, a suitable conduction band maximum, and good electrical conductivity, revealing its potential for application in the ETL of planar-heterojunction PSCs. Consequently, the ZrSnO4 ETL-based devices deliver promising power conversion efficiency (PCE) up to 19.05% from CH3NH3PbI3-based planar-heterojunction devices. Furthermore, the optimal ZrSnO4 ETL also contributes to decent long-term stability of the non-encapsulated device for 360 h in an ambient atmosphere (T~25 °C, RH~55%,), suggesting great potential of the sol-gel-driven ZrSnO4 thin film for a robust solution-processed ETL material in high-performance PSCs.
Frictional Pressure Drop and Cost Savings for Graphene Nanoplatelets Nanofluids in Turbulent Flow Environments
Authors: Mohammad Reem Sabah , Aldlemy Mohammed Suleman , Hassan Mu’ataz S. Al , Abdulla Aziz Ibrahim , Scholz Miklas , Yaseen Zaher Mundher
Published: November 2021

Covalent-functionalized graphene nanoplatelets (CF-GNPs) inside a circular heated-pipe and the subsequent pressure decrease loss within a fully developed turbulent flow were discussed in this research. Four samples of nanofluids were prepared and investigated in the ranges of 0.025 wt.%, 0.05 wt.%, 0.075 wt.%, and 0.1 wt.%. Different tools such as field emission scanning electron microscopy (FE-SEM), ultraviolet-visible-spectrophotometer (UV-visible), energy-dispersive X-ray spectroscopy (EDX), zeta potential, and nanoparticle sizing were used for the data preparation. The thermophysical properties of the working fluids were experimentally determined using the testing conditions established via computational fluid dynamic (CFD) simulations that had been designed to solve governing equations involving distilled water (DW) and nanofluidic flows. The average error between the numerical solution and the Blasius formula was ~4.85%. Relative to the DW, the pressure dropped by 27.80% for 0.025 wt.%, 35.69% for 0.05 wt.%, 41.61% for 0.075 wt.%, and 47.04% for 0.1 wt.%. Meanwhile, the pumping power increased by 3.8% for 0.025 wt.%, 5.3% for 0.05 wt.%, 6.6% for 0.075%, and 7.8% for 0.1 wt.%. The research findings on the cost analysis demonstrated that the daily electric costs were USD 214, 350, 416, 482, and 558 for DW of 0.025 wt.%, 0.05 wt.%, 0.075 wt.%, and 0.1 wt.%, respectively.