bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the impact of biological alterations in the superior thalamic radiations on exploratory eye movements in attenuated psychosis syndrome
Authors: Arai Yu , Katagiri Naoyuki , Tagata Hiromi , Uchino Takashi , Saito Junichi , Shido Yusuke , Kamiya Kouhei , Hori Masaaki , Mizuno Masafumi , Nemoto Takahiro
Published: June 2024

Introduction

Aberrant fixation and scan paths in visual searches have been repeatedly reported in schizophrenia. The frontal eye fields (FEF) and thalamus may be responsible for fixation and scan paths. These two regions are connected by superior thalamic radiation (STR) in humans. Studies have reported reduced fixation numbers and shortened scan path lengths in individuals with attenuated psychosis syndrome (APS) and schizophrenia. In this study, we hypothesized that STRs in the white matter fiber bundles of impairments underlie abnormalities in fixation and scan path length in individuals with APS.

Methods

Twenty-one individuals with APS and 30 healthy controls participated in this study. All participants underwent diffusion tensor imaging, and fractional anisotropy (FA) values of the left and right STR were analyzed using the novel method TractSeg. The number of eye fixations (NEF), total eye scanning length (TESL), and mean eye scanning length (MESL), derived using the exploratory eye movement (EEM) test, were adopted to evaluate the fixation and scan path length. We compared the FA values of the bilateral STR and EEM parameters between the APS and healthy control groups. We investigated the correlation between bilateral STR and EEM parameters in the APS and healthy control groups.

Results

NEF, TESL, MESL, and the FA values of the left STR were significantly reduced in individuals with APS compared to healthy controls. The left STR FA value in the APS group was significantly positively correlated with the MESL (r = 0.567, p = 0.007). In addition, the right STR FA value of the APS group was significantly correlated with the TESL (r = 0.587, p = 0.005) and MESL (r = 0.756, p = 0.7×10-4).

Discussion

These results demonstrate that biological changes in the STR, which connects the thalamus and FEF, underlie abnormalities in fixation and scanning. Recently, aberrations in the thalamus–frontal connection have been shown to underlie the emergence of psychotic symptoms. STR impairment may be a part of the biological basis of APS in individuals with subthreshold psychotic symptoms.
Annually mulched wild flower strips increase the observed wild bee (Apiformes) species richness and abundance in vineyard fallows in the short term
Authors: Krahner André , Maixner Michael , Porten Matthias , Schmitt Thomas
Published: June 2024

Insect conservation through habitat management is particularly important in regions of high biodiversity potential, such as steep-slope viticultural landscapes. Therefore, we studied the effects of realistic options for the conservation of open and flower-rich vineyard fallows on local wild bee communities. Using a fully-crossed factorial design, we sampled bees in 10 vineyard fallows in the Moselle region (SW Germany), which were partially subjected to different treatments (mulching, sowing of wild flower strips, combination, and untreated control). Over two years, we sampled 9323 individuals from 140 bee species with hand netting and pan traps. Based on the hand-netting samples, AICc-based model selection (Generalized Linear Mixed Models) revealed effects of fallow treatment, interacting with sampling year, on the number of sampled bee individuals and on the number of sampled bee species. In the second sampling year, we sampled significantly more individuals in wild flower strips (WFS) as well as in the combination treatment than in the untreated control. Also in the second year, the number of sampled species was significantly higher in the combination treatment than in the mulching treatment, and significantly higher than in the untreated control. Vineyard fallow management can promote bee communities in steep-slope viticultural landscapes, and options involving WFS, especially in combination with an annual mulching, should be preferred over options solely relying on repeated mulching. Moreover, the findings highlight that management options involving WFS need some time after initiation for unfolding their positive effects on bee communities.
Biomarkers in exposure-based treatment of anxiety in virtual reality: a systematic review
Authors: Ernst Mathias Torp , Hyldig Nielsen Jakob , Runge Eik , Bouchard Stéphane , Clemmensen Lars
Published: June 2024

Background

A large proportion of individuals with anxiety-related disorders refrain from seeking treatment. This may be because traditional exposure treatments induce anxiety. However, advances in exposure using virtual reality technology may encourage more individuals to seek treatment. Furthermore, using biomarkers with VR-based exposure may enable clinicians to assess anxiety levels objectively and collect data in a naturalistic setting.

Methods:

Here, we conduct a systematic review of the literature on the use of biomarkers in VR-based exposure treatment for anxiety. Twenty-seven studies were included, with a total of 1046 participants.

Results

We found that heart rate was the only biomarker that tentatively could identify changes within (75% of instances) and between sessions (60% of instances). The levels of synchrony between the findings for overall biomarkers and the results from questionnaires showed inconclusive results. Regarding the levels of synchrony between the findings for particular biomarkers and the results from questionnaires, only skin conductance level was highly synchronous for differences between groups (87% of instances).

Conclusion

Based on the present review, biomarkers cannot yet be used reliably to distinguish differences in self-reported symptoms of anxiety in VR-based exposure treatments.
Spatial profiles of magnetosheath parameters under different IMF orientations: THEMIS observations
Authors: Pi Gilbert , Němeček Zdeněk , Šafránková Jana , Grygorov Kostiantyn
Published: June 2024

Modification of the solar wind parameters at the bow shock (BS) and through the magnetosheath (MSH) is essential for the solar wind–magnetosphere interaction chain. The present study uses two approaches to determine the spatial profile of magnetic field strength and plasma parameters and their fluctuations along the Sun-Earth line under different interplanetary magnetic field (IMF) orientations with an emphasis on radial IMF conditions. The first method is based on the superposed epoch analysis of all the complete THEMIS MSH crossings between 2007 and 2010. The second approach uses the distance of the observing spacecraft from the model magnetopause (MP) expressed in units of an MSH thickness for all THEMIS observations. The results of both these analyses are consistent, and their comparison with simulations reveals the following features: 1) the sign of the IMF north-south component has a negligible effect on the spatial profile of the magnetic field strength or plasma parameters as well as on the level of fluctuations; 2) the ion temperature is enhanced for a radial MF and it is nearly isotropic throughout MSH; 3) the fluctuation level of plasma parameters just downstream BS is enhanced under a radial IMF, but it gradually decreases toward MP to a value typical for other IMF orientations; 4) magnetic field fluctuations are enhanced by a factor of 1.7 in the whole magnetosheath when IMF points radially.
Establishment of an acute arterial mesenteric ischaemia model in canines with an endovascular approach
Authors: Shi Yadong , Zhou Yangyi , Yuan Yuan , Kong Jie , Gong Maofeng , Chen Liang , He Xu , Su Haobo , Gu Jianping
Published: June 2024

Purpose

This study aimed to evaluate the feasibility of establishing an arterial acute mesenteric ischemia (AMI) model in canines using transcatheter autologous thrombus administration.

Materials and methods

Ten canines were divided into the experimental group (Group A, n = 5) and the sham group (Group B, n = 5). The canines in Group A received thrombus administration to the superior mesenteric artery (SMA) through a guiding catheter, while the canines in Group B received normal saline administration. Blood samples were collected and tested at baseline and 2 h after modelling. Canines in Group A underwent manual thromboaspiration after blood and intestine samples were collected. Ischaemic grades of intestinal mucosa were evaluated under light microscopes.

Results

The AMI models were successfully conducted in all canines without procedure-related vessel injury or death. At the 2-h follow-up, the high-sensitivity C-reactive protein and D-dimer in Group A were significantly higher than in Group B (5.72 ± 1.8 mg/L vs. 2.82 ± 1.5 mg/L, p = 0.024; 2.25 ± 0.8 μg/mL vs. 0.27 ± 0.10 μg/mL, p = 0.005; respectively). The mean histopathologic intestinal ischaemic grade in Group A was significantly higher than in Group B (2.4 ± 0.5 vs. 0.8 ± 0.4, p < 0.001). After a median of 2 times of thromboaspiration, 80% (4/5) of the canines achieved complete SMA revascularisation.

Conclusion

This experimental study demonstrated that establishing an arterial model in canines using endovascular approaches was feasible. The present model may play an important role in the investigation of endovascular techniques in the treatment of arterial AMI.
Study on Utilizing Mask R-CNN for Phenotypic Estimation of Lettuce’s Growth Status and Optimal Harvest Timing
Authors: Hou Lixin , Zhu Yuxia , Wei Ning , Liu Zeye , You Jixuan , Zhou Jing , Zhang Jian
Published: June 2024

Lettuce is an annual plant of the family Asteraceae. It is most often grown as a leaf vegetable, but sometimes for its stem and seeds, and its growth status and quality are evaluated based on its morphological phenotypic traits. However, traditional measurement methods are often labor-intensive and time-consuming due to manual measurements and may result in less accuracy. In this study, we proposed a new method utilizing RGB images and Mask R-Convolutional Neural Network (CNN) for estimating lettuce critical phenotypic traits. Leveraging publicly available datasets, we employed an improved Mask R-CNN model to perform a phenotypic analysis of lettuce images. This allowed us to estimate five phenotypic traits simultaneously, which include fresh weight, dry weight, plant height, canopy diameter, and leaf area. The enhanced Mask R-CNN model involved two key aspects: (1) replacing the backbone network from ResNet to RepVGG to enhance computational efficiency and performance; (2) adding phenotypic branches and constructing a multi-task regression model to achieve end-to-end estimation of lettuce phenotypic traits. Experimental results demonstrated that the present method achieved high accuracy and stable results in lettuce image segmentation, detection, and phenotypic estimation tasks, with APs for detection and segmentation being 0.8684 and 0.8803, respectively. Additionally, the R2 values for the five phenotypic traits are 0.96, 0.9596, 0.9329, 0.9136, and 0.9592, with corresponding mean absolute percentage errors (MAPEs) of 0.1072, 0.1522, 0.0757, 0.0548, and 0.0899, respectively. This study presents a novel technical advancement based on digital knowledge for phenotypic analysis and evaluation of lettuce quality, which could lay the foundation for artificial intelligence expiation in fresh vegetable production.
Selection of Improved Banana Diploid Resistant to Fusarium oxysporum f. sp. cubense Races 1 and Subtropical 4
Authors: Santana Welly Sacramento , Rocha Anelita de Jesus , da Silva Wesley Barreto , Amorim Vanusia Batista Oliveira de , Ramos Andresa Priscila de Souza , Haddad Fernando , Amorim Edson Perito
Published: June 2024

Bananas, a staple food globally and a key agricultural commodity, face a severe threat from the fungus Fusarium oxysporum f. sp. cubense (Foc), significantly impacting production. Genetic improvement to develop resistant cultivars stands out as a crucial strategy to mitigate this disease. This study focused on assessing and recommending enhanced diploid banana varieties for resistance against Foc subtropical race 4 (ST4) and Foc race 1 (R1). Twenty-four improved diploids developed by Embrapa, Brazil, underwent evaluation. Utilizing a scale for internal symptoms at 90 days after inoculation, genotypes were categorized from highly resistant to highly susceptible based on the internal symptom index. The diploid M53 exhibited high resistance to Foc R1 and resistance to Foc ST4, while only the diploid CNPMF 0534 demonstrated complete resistance to both R1 and ST4, with resistance to the latter likely associated with penetration, primarily due to the presence of callose. These findings provide valuable insights for banana and plantain breeding programs, offering selected diploids for crossbreeding with commercial cultivars to develop new, resistant genotypes against Foc.
Precision Inter-Row Relative Positioning Method by Using 3D LiDAR in Planted Forests and Orchards
Authors: Liu Limin , Ji Dong , Zeng Fandi , Zhao Zhihuan , Wang Shubo
Published: June 2024

Accurate positioning at the inter-row canopy can provide data support for precision variable-rate spraying. Therefore, there is an urgent need to design a reliable positioning method for the inter-row canopy of closed orchards (planted forests). In the study, the Extended Kalman Filter (EKF) fusion positioning method (method C) was first constructed by calibrating the IMU and encoder with errors. Meanwhile, 3D Light Detection and Ranging (LiDAR) observations were introduced to be fused into Method C. An EKF fusion positioning method (method D) based on 3D LiDAR corrected detection was designed. The method starts or closes method C by the presence or absence of the canopy. The vertically installed 3D LiDAR detected the canopy body center, providing the vehicle with inter-row vertical distance and heading. They were obtained through the distance between the center of the body and fixed row spacing. This can provide an accurate initial position for method C and correct the positioning trajectory. Finally, the positioning and canopy length measurement experiments were designed using a GPS positioning system. The results show that the method proposed in this study can significantly improve the accuracy of length measurement and positioning at the inter-row canopy, which does not significantly change with the distance traveled. In the orchard experiment, the average positioning deviations of the lateral and vertical distances at the inter-row canopy are 0.1 m and 0.2 m, respectively, with an average heading deviation of 6.75°, and the average relative error of canopy length measurement was 4.35%. The method can provide a simple and reliable inter-row positioning method for current remote-controlled and manned agricultural machinery when working in standardized 3D crops. This can modify the above-mentioned machinery to improve its automation level.
A Paleopathological Find on a La Tène Horse Skeleton Discovered in Rescue Archaeological Diggings in the Area of the Olympic Pool, Alba Iulia (CX 143 Pit)
Authors: Gudea Alexandru , Stan Florin Gheorghe , Irimie Alexandra , Mârza Sorin , Martonos Cristian Olimpiu , Gal Adrian
Published: June 2024

The reconstruction of past life based on archaeozoology is a challenging domain that offers a range of valuable details concerning former human and animal populations. Additionally, the ancient era is a source of information for human and veterinary medicine, as well as for other biological sciences. This report highlights a pathological lesion identified during the investigation of a horse skeleton from a pit dated La Tène in Alba County (Romania). The left scapula with lesions was collected from the skeleton of a buried 7–8-year-old male horse. The aforementioned bone underwent gross, radiological, and computerized tomography evaluation. Macroscopically, a collar of supraarticular cancellous hyperostosis was detected, most likely as a consequence of an invasive chronic phlegmonous periarthritis and/or bursitis of the infraspinate muscle following probably a penetrating cutaneous wound in the shoulder region. A suppurative periarthritis\bursitis of the infraspinate muscle situated nearby caused, apparently, supraglenoidian periosteitis responsible for osteophytes and exostoses formation in the neck region of the scapula.
Inhibition of Prolactin Affects Epididymal Morphology by Decreasing the Secretion of Estradiol in Cashmere Bucks
Authors: Liu Xiaona , Duan Chunhui , Yin Xuejiao , Zhang Lechao , Chen Meijing , Zhao Wen , Li Xianglong , Liu Yueqin , Zhang Yingjie
Published: June 2024

Yanshan Cashmere bucks are seasonal breeding animals and an important national genetic resource. This study aimed to investigate the involvement of prolactin (PRL) in the epididymal function of bucks. Twenty eleven-month-old Cashmere bucks were randomly divided into a control (CON) group and a bromocriptine (BCR, a prolactin inhibitor, 0.06 mg/kg body weight (BW)) treatment group. The experiment was conducted from September to October 2020 in Qinhuangdao City, China, and lasted for 30 days. Blood was collected on the last day before the BCR treatment (day 0) and on the 15th and 30th days after the BCR treatment (days 15 and 30). On the 30th day, all bucks were transported to the local slaughterhouse, where epididymal samples were collected immediately after slaughter. The left epididymis was preserved in 4% paraformaldehyde for histological observation, and the right epididymis was immediately preserved in liquid nitrogen for RNA sequencing (RNA-seq). The results show that the PRL inhibitor reduced the serum PRL and estradiol (E2) concentrations (p < 0.05) and tended to decrease luteinizing hormone (LH) concentrations (p = 0.052) by the 30th day, but no differences (p > 0.05) occurred by either day 0 or 15. There were no differences (p > 0.05) observed in the follicle-stimulating hormone (FSH), testosterone (T), and dihydrotestosterone (DHT) concentrations between the two groups. The PRL receptor (PRLR) protein was mainly located in the cytoplasm and intercellular substance of the epididymal epithelial cells. The PRL inhibitor decreased (p < 0.05) the expression of the PRLR protein in the epididymis. In the BCR group, the height of the epididymal epithelium in the caput and cauda increased, as did the diameter of the epididymal duct in the caput (p < 0.05). However, the diameter of the cauda epididymal duct decreased (p < 0.05). Thereafter, a total of 358 differentially expressed genes (DEGs) were identified in the epididymal tissues, among which 191 were upregulated and 167 were downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that ESR2, MAPK10, JUN, ACTL7A, and CALML4 were mainly enriched in the estrogen signaling pathway, steroid binding, calcium ion binding, the GnRH signaling pathway, the cAMP signaling pathway, and the chemical carcinogenesis–reactive oxygen species pathway, which are related to epididymal function. In conclusion, the inhibition of PRL may affect the structure of the epididymis by reducing the expression of the PRLR protein and the secretion of E2. ESR2, MAPK10, JUN, ACTL7A, and CALML4 could be the key genes of PRL in its regulation of epididymal reproductive function.
Defined Pig Microbiota Mixture as Promising Strategy against Salmonellosis in Gnotobiotic Piglets
Authors: Modrackova Nikol , Horvathova Kristyna , Mekadim Chahrazed , Splichal Igor , Splichalova Alla , Amin Ahmad , Mrazek Jakub , Vlkova Eva , Neuzil-Bunesova Vera
Published: June 2024

Probiotics are a potential strategy for salmonellosis control. A defined pig microbiota (DPM) mixture of nine bacterial strains previously exhibited probiotic and anti-Salmonella properties in vitro. Therefore, we evaluated its gut colonization ability and protection effect against S. typhimurium LT2-induced infection in the gnotobiotic piglet model. The DPM mixture successfully colonized the piglet gut and was stable and safe until the end of the experiment. The colon was inhabited by about 9 log CFU g−1 with a significant representation of bifidobacteria and lactobacilli compared to ileal levels around 7–8 log CFU g−1. Spore-forming clostridia and bacilli seemed to inhabit the environment only temporarily. The bacterial consortium contributed to the colonization of the gut at an entire length. The amplicon profile analysis supported the cultivation trend with a considerable representation of lactobacilli with bacilli in the ileum and bifidobacteria with clostridia in the colon. Although there was no significant Salmonella-positive elimination, it seems that the administered bacteria conferred the protection of infected piglets because of the slowed delayed infection manifestation without translocations of Salmonella cells to the blood circulation. Due to its colonization stability and potential protective anti-Salmonella traits, the DPM mixture has promising potential in pig production applications. However, advanced immunological tests are needed.
Optimizing Antibiotic Therapy for Stenotrophomonas maltophilia Infections in Critically Ill Patients: A Pharmacokinetic/Pharmacodynamic Approach
Authors: Barrasa Helena , Morán Miguel Angel , Fernández-Ciriza Leire , Isla Arantxa , Solinís María Ángeles , Canut-Blasco Andrés , Rodríguez-Gascón Alicia
Published: June 2024

Stenotrophomonas maltophilia is an opportunistic, multidrug-resistant non-fermentative Gram-negative bacillus, posing a significant challenge in clinical treatment due to its numerous intrinsic and acquired resistance mechanisms. This study aimed to evaluate the adequacy of antibiotics used for the treatment of S. maltophilia infections in critically ill patients using a pharmacokinetic/pharmacodynamic (PK/PD) approach. The antibiotics studied included cotrimoxazole, levofloxacin, minocycline, tigecycline, cefiderocol, and the new combination aztreonam/avibactam, which is not yet approved. By Monte Carlo simulations, the probability of target attainment (PTA), the PK/PD breakpoints, and the cumulative fraction of response (CFR) were estimated. PK parameters and MIC distributions were sourced from the literature, the European Committee on Antimicrobial Susceptibility Testing (EUCAST), and the SENTRY Antimicrobial Surveillance Program collection. Cefiderocol 2 g q8h, minocycline 200 mg q12h, tigecycline 100 mg q12h, and aztreonam/avibactam 1500/500 mg q6h were the best options to treat empirically infections due to S. maltophilia. Cotrimoxazole provided a higher probability of treatment success for the U.S. isolates than for European isolates. For all antibiotics, discrepancies between the PK/PD breakpoints and the clinical breakpoints defined by EUCAST (or the ECOFF) and CLSI were detected.
Genetic and Phenotypic Changes Related to the Development of mec-Independent Oxacillin Non-Susceptibility in ST8 Staphylococcus aureus Recovered after Antibiotic Therapy in a Patient with Bacteremia
Authors: Di Gregorio Sabrina , Weltman Gabriela , Fabbri Carolina , Fernández Silvina , Zárate Soledad , Smayevsky Jorgelina , Power Pablo , Campos Josefina , Llarrull Leticia Irene , Mollerach Marta
Published: June 2024

The mec-independent oxacillin non-susceptible S. aureus (MIONSA) strains represent a great clinical challenge, as they are not easily detected and can lead to treatment failure. However, the responsible molecular mechanisms are still very little understood. Here, we studied four clinical ST8-MSSA-t024 isolates recovered during the course of antibiotic treatment from a patient suffering successive episodes of bacteremia. The first isolates (SAMS1, SAMS2, and SAMS3) were susceptible to cefoxitin and oxacillin. The last one (SA2) was susceptible to cefoxitin, resistant to oxacillin, lacked mec genes, and had reduced susceptibility to teicoplanin. SA2 showed higher β-lactamase activity than SAMS1. However, β-lactamase hyperproduction could not be linked to oxacillin resistance as it was not inhibited by clavulanic acid, and no genetic changes that could account for its hyperproduction were found. Importantly, we hereby report the in vivo acquisition and coexistence of different adaptive mutations in genes associated with peptidoglycan synthesis (pbp2, rodA, stp1, yjbH, and yvqF/vraT), which is possibly related with the development of oxacillin resistance and reduced susceptibility to teicoplanin in SA2. Using three-dimensional models and PBP binding assays, we demonstrated the high contribution of the SA2 PBP2 Ala450Asp mutation to the observed oxacillin resistance phenotype. Our results should be considered as a warning for physicians and microbiologists in the region, as MIONSA detection and treatment represent an important clinical challenge.
Mechanistic Study of Antimicrobial Effectiveness of Cyclic Amphipathic Peptide [R4W4] against Methicillin-Resistant Staphylococcus aureus Clinical Isolates
Authors: Akinwale Ajayi David , Parang Keykavous , Tiwari Rakesh Kumar , Yamaki Jason
Published: June 2024

Antimicrobial peptides (AMPs) are being explored as a potential strategy to combat antibiotic resistance due to their ability to reduce susceptibility to antibiotics. This study explored whether the [R4W4] peptide mode of action is bacteriostatic or bactericidal using modified two-fold serial dilution and evaluating the synergism between gentamicin and [R4W4] against Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA) by a checkered board assay. [R4W4] exhibited bactericidal activity against bacterial isolates (MBC/MIC ≤ 4), with a synergistic effect with gentamicin against E. coli (FICI = 0.3) but not against MRSA (FICI = 0.75). Moreover, we investigated the mechanism of action of [R4W4] against MRSA by applying biophysical assays to evaluate zeta potential, cytoplasmic membrane depolarization, and lipoteichoic acid (LTA) binding affinity. [R4W4] at a 16 mg/mL concentration stabilized the zeta potential of MRSA −31 ± 0.88 mV to −8.37 mV. Also, [R4W4] at 2 × MIC and 16 × MIC revealed a membrane perturbation process associated with concentration-dependent effects. Lastly, in the presence of BODIPY-TR-cadaverine (BC) fluorescence dyes, [R4W4] exhibited binding affinity to LTA comparable with melittin, the positive control. In addition, the antibacterial activity of [R4W4] against MRSA remained unchanged in the absence and presence of LTA, with an MIC of 8 µg/mL. Therefore, the [R4W4] mechanism of action is deemed bactericidal, involving interaction with bacterial cell membranes, causing concentration-dependent membrane perturbation. Additionally, after 30 serial passages, there was a modest increment of MRSA strains resistant to [R4W4] and a change in antibacterial effectiveness MIC [R4W4] and vancomycin by 8 and 4 folds with a slight change in Levofloxacin MIC 1 to 2 µg/mL. These data suggest that [R4W4] warrants further consideration as a potential AMP.
Intelligent Fault Diagnosis of Rolling Bearings Based on Markov Transition Field and Mixed Attention Residual Network
Authors: Tong Anshi , Zhang Jun , Wang Danfeng , Xie Liyang
Published: June 2024

To address the problems of existing methods that struggle to effectively extract fault features and unstable model training using unbalanced data, this paper proposes a new fault diagnosis method for rolling bearings based on a Markov Transition Field (MTF) and Mixed Attention Residual Network (MARN). The acquired vibration signals are transformed into two-dimensional MTF feature images as network inputs to avoid the loss of the original signal information, while retaining the temporal correlation; then, the mixed attention mechanism is inserted into the residual structure to enhance the feature extraction capability, and finally, the network is trained and outputs diagnostic results. In order to validate the feasibility of the MARN, other popular deep learning (DL) methods are compared on balanced and unbalanced datasets divided by a CWRU fault bearing dataset, and the proposed method results in superior performance. Ultimately, the proposed method achieves an average recognition accuracy of 99.5% and 99.2% under the two categories of divided datasets, respectively.
Novel Algorithm to Detect, Classify, and Count Mussel Larvae in Seawater Samples Using Computer Vision
Authors: Orgeira-Crespo Pedro , Gabín-Sánchez Carlos , Aguado-Agelet Fernando , Rey-González Guillermo
Published: June 2024

The European Union’s mussel production industry is dependent on obtaining mussel larvae as seed for cultivation, a process traditionally monitored through labor-intensive manual sampling and microscopic counting prone to human error and time-consuming procedures. To address these challenges, our research presents a computer vision-based methodology for accurately identifying, classifying, and quantifying mussel larvae individuals across various developmental stages from microscopic images of water samples. Utilizing a neural network architecture derived from the YOLO method, our approach integrates convolutional, pooling, and fully connected layers to automate detection, classification, and accounting tasks. Through training with manually labeled samples and employing data augmentation techniques, we established a robust framework capable of processing diverse larval specimens effectively. Our research not only streamlines mussel larvae monitoring processes but also underscores the potential of computer vision techniques to enhance efficiency and accuracy in aquaculture industries.
Optimization Method for Assembly Sequence Evaluation Based on Assembly Cost and Ontology of Aviation Reducers
Authors: Liu Peng , Wu Linfeng , Wang Yanzhong , Guo Lize
Published: June 2024

An assembly sequence evaluation is one of the most important research directions of assembly sequence planning (ASP) for complex mechanical transmission products. Currently, aviation reducers lack a multi-perspective and multi-level evaluation of their assembly sequence. The existing evaluation indicators vary. The evaluation methods have low effectiveness and poor practicability. Therefore, a comprehensive multidimensional evaluation method for complex assembly sequences is proposed in this paper. A multidimensional comprehensive evaluation of the overall assembly quality and performance indices of aviation reducer products is realized. Firstly, the main factors affecting assembly sequence planning are considered: the attributes of the basic unit parts and the cost control of the assembly process. An evaluation index system of assembly sequence planning based on the two dimensions of assembly cost and ontology is constructed. Then, according to the multidimensional evaluation index, fuzzy evaluation theory is used to establish a fuzzy set and a matrix for each dimensional evaluation index. The index weight is divided. A comprehensive evaluation model and the function of each dimension are established. After a comprehensive evaluation, the multidimensional assembly sequence evaluation method for aviation reducers is formed. Finally, the method is applied to the assembly process of the primary reducer of a helicopter’s main reducer, and a comprehensive evaluation of its assembly sequence scheme is completed to verify the feasibility of the proposed method. This article constructs a complex assembly sequence evaluation method that includes 12 evaluation indicators, improves the assembly sequence planning evaluation index system of aviation reducers, and can effectively promote the progress of optimization technology for complex assembly sequences of aviation reducers.
The Thermodynamic Change Laws of CO2-Coupled Fractured Rock
Authors: Yu Fei , Deng Guangzhe
Published: June 2024

Under the background of the “dual carbon” target, exploring the pathway of efficient geological storage and high energy utilization of CO2 is a hot issue in CO2 emission reduction research. Under the coupling effect of high geopathic stress in deep rock layers and thermal stress generated during the geological sequestration of CO2, CO2 infiltration into coal rock changes the ambient temperature around the rock, while thermal diffusion effects cause damage to the rock and influence fracture expansion. In the present study, CO2-water–rock coupling test system and characterization of fissure surface roughness were conducted to analyze the rock’s mechanical properties, damage, and fracture evolution. Modeling of the equivalent fissure was employed to reveal the heat transfer mechanism between the rock matrix and CO2. The results obtained illustrate that the rock samples coupled with CO2 exhibited remarkable changes in mechanical properties. These changes include an increase in the number of pores, enhanced inter-pore connectivity, and a planar type of surface roughness in the fissures, ultimately resulting in an increase in conductivity. Conversely, the remaining rock samples displayed poor mechanical properties and surface fracture connectivity. As pressure decreased, the heat transfer coefficient decreased from 86.9 W/m2·K to 57.5 W/m2·K, accompanied by a temperature drop from 33.6 °C to 30.6 °C, demonstrating a proportional relationship between pressure and the heat transfer coefficient. Furthermore, the flow rate gradually increased with the rise in CO2 pressure, indicating denser flow lines with faster flow rates. At 15 MPa, CO2 exhibits enhanced mobility.
Risk Assessment of Landfalling Tropical Cyclones in China Based on Hazard Risk Theory
Authors: Xu Jin , Xue Xinyue , Yang Bo , Wang Wen , Wu Wenxiang , Ji Xiaodong
Published: June 2024

As a frequent hazard, tropical cyclones have a great impact on the social and economic development of China, which is close to the origin of tropical cyclones in the western Pacific Ocean. The primary objective of this study was to construct a comprehensive risk assessment model for tropical cyclone hazards based on natural influencing factors, informing recommendations for hazard prevention and mitigation in affected regions. This research focused on tropical cyclones that made landfall in mainland China and Hainan from 1949 to 2023, utilizing hazard risk theory and classical extreme value theory. The wind speed and rainfall data during the peak cyclone periods (June to October) from 1997 to 2021 gathered from various meteorological stations, as well as altitude and vegetation cover data, were examined. Hierarchical analysis and ArcGIS spatial analysis methods were employed to study the characteristics of the spatiotemporal distribution of landfalling tropical cyclones and the comprehensive risk of tropical cyclone hazards, and the regions of China were delineated according to these methods. The results showed that, during the period from 1949 to 2023, the overall number of landfalling tropical cyclones decreased in a fluctuating manner, while the intensity of the cyclones increased. Furthermore, severe typhoons tended to occur more frequently in the summer than autumn with time, intensifying the challenge to resist short-term hazards. Moreover, the hazard-causing factors in areas affected by tropical cyclones displayed an increasing trend from north to south and from west to east. In detail, the regions sensitive to natural hazards were primarily located in the central part of Liaoning province, Tianjin, central and eastern Hebei province, Shandong province, eastern Henan province, central and northern Anhui province, Jiangsu province, and Shanghai, which are characterized by flat terrain and relatively low vegetation cover. Overall, the comprehensive risk of tropical cyclone hazards showed a geographical distribution that decreases from south to north and from east to west, with coastal cities in provinces such as Hainan, Guangdong, Guangxi, Fujian, and Zhejiang—including Haikou, Zhanjiang, Xiamen, Beihai, and Taizhou—exhibiting the highest levels of risk.
Durability Assessment of Eco-Friendly Bricks Containing Lime Kiln Dust and Tire Rubber Waste Using Mercury Intrusion Porosimetry
Authors: Oke Joy Ayankop , Abuel-Naga Hossam
Published: June 2024

The global challenge faced due to the impact of the construction industry on climate change, along with the issues surrounding sustainable waste disposal, has necessitated various research on using waste products as eco-friendly alternatives in construction. In this study, the avoidance of waste disposal through landfills in Australia was encouraged by incorporating lime kiln dust (LKD) and tire rubber waste (TRW) into masonry mixes to manufacture green bricks. Furthermore, the investigations in this article highlight the use of mercury intrusion porosimetry (MIP) to determine the durability of the LKD-TRW bricks when exposed to freeze–thaw (F-T) cycles by examining the pore size distribution within the bricks. The LKD waste was blended with ground granulated blast furnace slag (GGBFS) at a 70:30 blending ratio and combined with the TRW in stepped increments of 5% from 0 to 20% to produce these eco-friendly bricks. The compressive strength (CS), flexural strength (FS), frost resistance (FR), pore size distribution according to mercury intrusion porosimetry (MIP), and the water absorption (WA) properties of the bricks were assessed. The CS and FS values at 28 days of curing were recorded as 6.17, 5.25, and 3.09 MPa and 2.52, 2, and 1.55 MPa for 0, 5, and 10% TRW contents, respectively. Durability assessments using the F-T test showed that the bricks produced with 0% TRW passed as frost-resistant bricks. Furthermore, the results from the MIP test showed a total pore volume of 0.033 mL/g at 3 µm pore size for the 0% TRW content, further confirming its durability. Hence, the 0% LKD-TRW bricks can be utilized in cold regions where temperatures can be as low as −43 °C without deteriorating. Lastly, WA values of 7.25, 11.76, and 14.96% were recorded for the bricks with 0, 5, and 10% TRW, respectively, after the 28-day curing period. From all of the results obtained from the laboratory investigations, the LKD-TRW bricks produced with up to 10% TRW were within the satisfactory engineering requirements for masonry units.
Simulation Analysis of Cyclone Separator for Separation of Cenospheres
Authors: Shen Rui , He Ping , Yoriya Sorachon , Chen Naichao , Wu Jiang , Hu Tianyang , He Kangsai , Cai Qian
Published: June 2024

The separation of cenospheres is an important method for fly ash recycling, and there is no efficient and less polluting method available for separating cenospheres. This paper proposes the dry separation of cenospheres using a cyclone separator, which utilizes the small density of cenospheres as a feature to separate them. The corresponding numerical simulations of the cyclone separator model used to separate the cenospheres were carried out by a CFD analysis, after which the size of the radius of the upper discharge opening and the height of the sloping wall of the cyclone separator were analyzed by simulation; the effect of the incident velocity on the Newton’s efficiency of the separation of the cenospheres was also analyzed. The simulation results indicate that the Newton’s efficiency can reach 0.55 when the radius of the upper feed port is 90 mm, the height of the inclined wall is 400 mm, and the incident speed is 2.5 m/s.
The Interaction of Cytokines in Orthodontics: A Systematic Review
Authors: Inchingolo Francesco , Inchingolo Angelo Michele , Malcangi Giuseppina , Ferrante Laura , Trilli Irma , Di Noia Angela , Piras Fabio , Mancini Antonio , Palermo Andrea , Inchingolo Alessio Danilo , Dipalma Gianna
Published: June 2024

Aim: Cytokines are crucial low-molecular-weight proteins involved in immune responses. This systematic review highlights the need for in-depth studies on cytokines’ biological mechanisms, providing insights into disease onset and potential therapeutic strategies. Materials and methods: A comprehensive literature search identified 18 relevant articles, emphasizing the multifaceted role of cytokines in orthodontic treatment (OT). The quality assessment using the ROBINS-I tool ensures a rigorous evaluation of the included studies, contributing to the overall reliability of the findings. Results and Conclusions: This systematic review explores the intricate relationship between cytokines and OT. Cytokines exhibit different properties, influencing cellular activities through autocrine, paracrine, and endocrine activities. OT, aimed at achieving stable occlusion, induces tension and compression in the periodontal ligament (PDL), triggering cytokine release. Proinflammatory cytokines play a role in inflammation, influencing bone and soft tissue metabolism. Studies show elevated cytokine levels in gingival crevicular fluid (GCF) after orthodontic force application. The choice of orthodontic devices, such as self-ligating brackets, influences cytokine concentrations, indicating the importance of attachment design. Further research promises to enhance orthodontic practices, and optimize patient care.
Exhaustive Variant Interaction Analysis Using Multifactor Dimensionality Reduction
Authors: Gómez-Sánchez Gonzalo , Alonso Lorena , Pérez Miguel Ángel , Morán Ignasi , Torrents David , Berral Josep Ll.
Published: June 2024

One of the main goals of human genetics is to understand the connections between genomic variation and the predisposition to develop a complex disorder. These disease–variant associations are usually studied in a single independent manner, disregarding the possible effect derived from the interaction between genomic variants. In particular, in a background of complex diseases, these interactions can be directly linked to the disorder and may play an important role in disease development. Although their study has been suggested to help complete the understanding of the genetic bases of complex diseases, this still represents a big challenge due to large computing demands. Here, we take advantage of high-performance computing technologies to tackle this problem by using a combination of machine learning methods and statistical approaches. As a result, we created a containerized framework that uses multifactor dimensionality reduction (MDR) to detect pairs of variants associated with type 2 diabetes (T2D). This methodology was tested on the Northwestern University NUgene project cohort using a dataset of 1,883,192 variant pairs with a certain degree of association with T2D. Out of the pairs studied, we identified 104 significant pairs: two of which exhibit a potential functional relationship with T2D. These results place the proposed MDR method as a valid, efficient, and portable solution to study variant interaction in real reduced genomic datasets.
Segmentation of Liver Tumors by Monai and PyTorch in CT Images with Deep Learning Techniques
Authors: Muhammad Sabir , Zhang Jing
Published: June 2024

Image segmentation and identification are crucial to modern medical image processing techniques. This research provides a novel and effective method for identifying and segmenting liver tumors from public CT images. Our approach leverages the hybrid ResUNet model, a combination of both the ResNet and UNet models developed by the Monai and PyTorch frameworks. The ResNet deep dense network architecture is implemented on public CT scans using the MSD Task03 Liver dataset. The novelty of our method lies in several key aspects. First, we introduce innovative enhancements to the ResUNet architecture, optimizing its performance, especially for liver tumor segmentation tasks. Additionally, by harassing the capabilities of Monai, we streamline the implementation process, eliminating the need for manual script writing and enabling faster, more efficient model development and optimization. The process of preparing images for analysis by a deep neural network involves several steps: data augmentation, a Hounsfield windowing unit, and image normalization. ResUNet network performance is measured by using the DC metric Dice coefficient. This approach, which utilizes residual connections, has proven to be more reliable than other existing techniques. This approach achieved DC values of 0.98% for detecting liver tumors and 0.87% for segmentation. Both qualitative and quantitative evaluations show promising results regarding model precision and accuracy. The implications of this research are that it could be used to increase the precision and accuracy of liver tumor detection and liver segmentation, reflecting the potential of the proposed method. This could help in the early diagnosis and treatment of liver cancer, which can ultimately improve patient prognosis.
Numerical Simulation of the Influence of the Baihetan Reservoir Impoundment on Regional Seismicity
Authors: Wang Zitao , Zhang Huai , Guo Yicun , Meng Qiu
Published: June 2024

The Baihetan Reservoir is built for hydropower in China. The rise of the reservoir water leads to a series of earthquakes in the surrounding area. This study proposes fully coupled equations of pore-viscoelasticity and a parallel partition mesh model to study the short- and long-term effects of the Baihetan Reservoir and further calculate the changes in stress, pore pressure, and Coulomb failure stress with time on the major faults. Based on the calculation results, impoundment increases regional seismicity, which is consistent with the seismic catalog. The reservoir impoundment causes an increase in pore pressure in the crust, primarily enhancing Coulomb failure stress beneath the reservoir center. This effect extends to approximately 60 km in length and 20 km in width at a depth layer of 5–10 km. Seismicity varies greatly among different faults. Coulomb failure stress increases on the northern part of the Xiaojiang Fault and Zhaotong-Ludian Fault, and decreases on the southern part of the Xiaojiang Fault and Zemuhe Fault. The Coulomb failure stress is highly correlated with the number of earthquakes along the Xiaojiang Fault. The influence of the reservoir on the local seismicity is mainly limited to several months, and it has a slight effect later on. The focal depth of the induced earthquakes increases while the magnitude decreases. The earthquakes caused by the impoundment all have a small magnitude, and the Ms4.3 Qiaojia earthquake on 30 March 2022, was more likely a natural event.
Strength and Deformation Analyses of Selected Filaments for Large-Format Additive Manufacturing Applicable to the Production of Firefighting Water Tanks
Authors: Hnilicová Michaela , Kotšmíd Stanislav , Dado Miroslav , Hnilica Richard
Published: June 2024

Large-format additive manufacturing is a candidate for tremendous savings in terms of time and cost while simultaneously enabling higher flexibility, quality, and variability. Most of the design constraints of small-scale polymer 3D printers still apply to large-format additive manufacturing. The paper details both the strengths and deformation-related design considerations for additive manufacturing to gain a better understanding of the material capabilities and limitations, mechanical characteristics, and how to use them for large-format additive manufacturing (LFAM). The results show that the tested materials for additive manufacturing meet the requirements from the stress and deformation points of view. Compared to the steel and composite material, the strength limits are lower, but high enough for the given load. The materials HI TEMP, HI TEMP CF, PA12CF, PA6/66, and PLA seem to be the most promising for LFAM to create a firefighting water tank. The results may be considered as an introduction to further research that should lead to real solutions for the production of atypical tanks.