bluuebunny/crossref_metadata_2025 huggingface dataset:
Salmonella infection among the pediatric population at a tertiary care children’s hospital in central Nepal: a retrospective study
Authors: Pokhrel Nayanum , Chapagain Ramhari , Thakur Chandan Kumar , Basnet Ajaya , Amatya Isha , Singh Rajan , Ghimire Raghav
Published: September 2023

Background

Typhoid fever, an infective bacterial disease, is capable of causing fatal systemic infection in humans, and in an era of antimicrobial resistance, it has become of public health importance. This study aimed to investigate the laboratory diagnosis of Salmonella bloodstream infection, its serotype, antimicrobial resistance pattern, and seasonal variation at a tertiary care children’s hospital.

Methods

We undertook a retrospective, cross-sectional study by reviewing hospital-based laboratory records of patients whose blood culture samples were submitted from the outpatient department to the laboratory of a tertiary care children’s hospital in Kathmandu, Nepal, from January 2017 to January 2019.

Results

Among the total blood culture samples obtained (n = 39,771), bacterial isolates (n = 1,055, 2.65%) belonged either to the Genus Enterobacteriaceae or Genus Acinetobacter. Altogether (n = 91, 8.63%), isolates were positive for Salmonella spp., which were further identified as Salmonella enterica subsp. enterica ser. Typhi (n = 79, 7.49%), Salmonella enterica subsp. enterica ser. Paratyphi A (n = 11, 1.04%), and Salmonella enterica subsp. enterica ser. Paratyphi B (n = 1, 0.1%). The median age of patients was 6  years (IQR: 4–9), with male and female patients constituting (n = 53, 58.24%; OR, 1.0; 95% CI, 0.60–1.67) and (n = 38, 41.76%; OR, 0.98; 95% CI, 0.49–2.05) cases, respectively. The disease was observed throughout the year, with a high prevalence toward the spring season (March–May). An antibiogram showed resistance more toward nalidixic acid with S. Typhi, comprising half the isolates (n = 52, 65.82%; p = 0.11). Resistance toward β-lactams with β-lactamase inhibitors (amoxicillin/clavulanate; 1.27%) was seen in a single isolate of S. Typhi. The multidrug resistance pattern was not pronounced. The multiple antibiotic resistance (MAR) index was in the range between 0.14 and 0.22 in S. Typhi and 0.22 and 0.23 in S. Paratyphi.

Conclusion

Salmonella Typhi was the predominant ser. Infection was common among children between 1 and 5 years of age, showing male predominance and with the spring season contributing to a fairly higher number of cases. Antimicrobial susceptibility testing of S. Typhi showed more resistance toward nalidixic acid, with only a single isolate resistant to β-lactamase inhibitors (amoxicillin/clavulanate). Alarming multidrug resistance patterns were not observed. The MAR index in this study indicates the importance of the judicious use of antimicrobials and hospital infection prevention and control practices.
“Influence of plasmids, selection markers and auxotrophic mutations on Haloferax volcanii cell shape plasticity”
Authors: Patro Megha , Duggin Iain G. , Albers Sonja-Verena , Ithurbide Solenne
Published: September 2023

Haloferax volcanii and other Haloarchaea can be pleomorphic, adopting different shapes, which vary with growth stages. Several studies have shown that H. volcanii cell shape is sensitive to various external factors including growth media and physical environment. In addition, several studies have noticed that the presence of a recombinant plasmid in the cells is also a factor impacting H. volcanii cell shape, notably by favoring the development of rods in early stages of growth. Here we investigated the reasons for this phenomenon by first studying the impact of auxotrophic mutations on cell shape in strains that are commonly used as genetic backgrounds for selection during strain engineering (namely: H26, H53, H77, H98, and H729) and secondly, by studying the effect of the presence of different plasmids containing selection markers on the cell shape of these strains. Our study showed that most of these auxotrophic strains have variation in cell shape parameters including length, aspect ratio, area and circularity and that the plasmid presence is impacting these parameters too. Our results indicated that ΔhdrB strains and hdrB selection markers have the most influence on H. volcanii cell shape, in addition to the sole presence of a plasmid. Finally, we discuss limitations in studying cell shape in H. volcanii and make recommendations based on our results for improving reproducibility of such studies.
Culturing cells for life: innovative approaches in macroscopic and microfluidic cultures, with an emphasis on stem cells
Authors: Badilescu Simona , Parimalam Subhathirai Subramaniyan , Packirisamy Muthukumaran
Published: September 2023

In 2006, Whitesides, writing about microfluidics, said that microfluidics is in early adolescence and it is not yet clear how it will develop. Today, almost 20 years later, microfluidics became a fully developed, highly sophisticated, multidisciplinary field that had entirely honoured its early promise. Its strength stems from the knowledge and know-how, coming from multiple disciplines such as physics of fluids, engineering, and microfabrication in the beginning, followed, more recently, by cell biological research, in full bloom nowadays. In microfluidic devices, the environment of cells such as chemical and mechanical gradients can be reproduced, making biological studies even more compelling. The red thread of this review paper follows the new insights and discoveries in both traditional macro- and microfluidic cell culture brought into the cell biology field, especially in the culture of stem cells, filled with promise in the field of regenerative medicine. Microfluidic devices provide an environment that is much closer to that of in vivo cell culture than the conventional culture platforms, where large amounts of cells are cultured and the environment of individual cells cannot be distinguished. The convenience of live cell imaging, portability, and the integration of sensors to precisely, control various parameters, has expanded cell biologists’ arsenal In addition, microfluidic devices, integrated with different functionalities, that is, the automated cell culture systems, will be discussed as well.
Effects of vitamin D supplementation on maximal strength and power in athletes: a systematic review and meta-analysis of randomized controlled trials
Authors: Sist Marco , Zou Lu , Galloway Stuart D. R. , Rodriguez-Sanchez Nidia
Published: September 2023

Background

Vitamin D is thought to be a powerful modulator of skeletal muscle physiology. However, available data on the effects of vitamin D supplementation on muscle function in athletes are limited and with mixed results. This meta-analysis therefore, aimed to quantitatively summarize the up-to-date literature assessing the effects of vitamin D supplementation on muscle strength and power in athletes.

Methods

Sport Discus, PubMed, Cochrane Library and Web of Science were searched to identify randomized controlled trials (RCTs) that used one-repetition maximum (1RM) tests to assess maximal strength, and vertical jump to assess muscle power in athletes. The Cochrane Risk of Bias tool was used to evaluate the included RCTs for sources of bias. The standardized mean difference (SMD) was used as the effect size, interpreted together with its 95% confidence intervals (CI). The effect sizes were calculated on the changes from baseline between vitamin D and placebo groups for maximal strength results by upper body and lower body, and for power results.

Results

Eleven RCTs involving 436 athletes were included. The results indicated that if baseline serum 25(OH)D concentration was < 75 nmol/L, the treatment had a small effect on upper body muscle strength [SMD 0.25, 95% CI: (−0.44, 0.95), p = 0.47] and on lower body muscle strength [SMD 0.26, 95% CI: (−0.13, 0.65), p = 0.19]; if the baseline serum 25(OH)D concentration was ≥ 75 nmol/L, the treatment had a trivial effect on muscle power [SMD 0.15, 95% CI: (−0.42, 0.72), p = 0.61].

Discussion

This meta-analysis demonstrated that there is not a statistically significant effect of vitamin D supplementation on improving maximum strength and power, but highlights that further research is required addressing the key limitations in previous studies before definitive conclusions can be made.
Psychosocial drivers influencing local food purchasing: beyond availability, the importance of trust in farmers
Authors: Carfora Valentina , Catellani Patrizia
Published: September 2023

Introduction

Although consumers bought more local food during the changing context of pandemic COVID -19, this positive modification may not become a stable habit afterward.

Methods

To understand this change in drivers of consumers' intention to buy local food, we investigated the role of perceptions of various intrinsic and extrinsic attributes of local food, its perceived quality, price and availability, and consumers' trust in local food producers. We also investigated the role of sociodemographic variables as well as the moderating role of consumers' stage of change (i.e., absence, reduction, maintenance, and increase) in the purchase of local food.

Results

Structural equation modeling results on a representative sample of Italian consumers (N = 511) showed that local food availability is the main driver of purchase intention (β = 0.20; p = 0.001), especially among consumers who have changed their habits toward buying local food (reduction stage = β = 0.24; increase stage = 0.30; p = 0.001). In addition, trust in local food producers was found to be a key antecedent to consumers' perceptions of local food as environmentally friendly (β = 0.57; p = 0.001), healthy (β = 0.55; p = 0.001), authentic (β = 0.58; p = 0.001), tasty (β = 0.52; p = 0.001), socially sustainable (β = 0.59; p = 0.001), and as a product with a good appearance (β = 0.55; p = 0.001).

Discussion

Overall, these results improve our understanding of which food attributes should be emphasized in communication to promote the purchase of local food.
Short-chain fatty acids levels in human milk are not affected by holder pasteurization and high hydrostatic pressure processing
Authors: Marousez Lucie , Ichou Farid , Lesnik Philippe , Tran Léa Chantal , De Lamballerie Marie , Gottrand Frédéric , Ley Delphine , Lesage Jean
Published: September 2023

Sterilized donor milk (DM) is frequently used for feeding preterm infants. To date, the effect of different modes of DM sterilization on short-chain fatty acids (SCFAs) remains unknown. We aimed to quantify SCFAs in DM samples after two types of milk sterilization: the Holder pasteurization (HoP) and a high hydrostatic pressure (HP) processing. Eight pooled DM samples were sterilized by HoP (62.5°C for 30 min) or processed by HP (350 MPa at 38°C). Raw DM was used as control. Six SCFAs were quantified by gas chromatography/mass spectrometry. Compared to raw milk, both HoP and HP treatment did not significantly modulate the concentration of acetate, butyrate, propionate and isovalerate in DM. Valerate and isobutyrate were undetectable in DM samples. In conclusion, both HoP and HP processing preserved milk SCFAs at their initial levels in raw human milk.
End organ perfusion and pediatric microcirculation assessment
Authors: Arteaga Grace M. , Crow Sheri
Published: September 2023

Cardiovascular instability and reduced oxygenation are regular perioperative critical events associated with anesthesia requiring intervention in neonates and young infants. This review article addresses the current modalities of assessing this population's adequate end-organ perfusion in the perioperative period. Assuring adequate tissue oxygenation in critically ill infants is based on parameters that measure acceptable macrocirculatory hemodynamic parameters such as vital signs (mean arterial blood pressure, heart rate, urinary output) and chemical parameters (lactic acidosis, mixed venous oxygen saturation, base deficit). Microcirculation assessment represents a promising candidate for assessing and improving hemodynamic management strategies in perioperative and critically ill populations. Evaluation of the functional state of the microcirculation can parallel improvement in tissue perfusion, a term coined as “hemodynamic coherence”. Less information is available to assess microcirculatory disturbances related to higher mortality risk in critically ill adults and pediatric patients with septic shock. Techniques for measuring microcirculation have substantially improved in the past decade and have evolved from methods that are limited in scope, such as velocity-based laser Doppler and near-infrared spectroscopy, to handheld vital microscopy (HVM), also referred to as videomicroscopy. Available technologies to assess microcirculation include sublingual incident dark field (IDF) and sublingual sidestream dark field (SDF) devices. This chapter addresses (1) the physiological basis of microcirculation and its relevance to the neonatal and pediatric populations, (2) the pathophysiology associated with altered microcirculation and endothelium, and (3) the current literature reviewing modalities to detect and quantify the presence of microcirculatory alterations.
Prevalence of suicidal behavior in patients with chronic pain: a systematic review and meta-analysis of observational studies
Authors: Kwon Chan-Young , Lee Boram
Published: September 2023

Objective

Chronic pain is a leading cause of disability, severely impairing an individual’s daily activity and quality of life. In addition, this condition may contribute to suicidal thoughts by leading to neuropsychological impairments, a perceived lack of meaning in life, and pain-related catastrophizing. This systematic review aimed to comprehensively investigate the prevalence and associated factors of suicidal behaviors (SBs) including suicidal ideation (SI) and suicide attempt (SA) or its complete, in individuals with chronic pain.

Methods

Five electronic databases were searched up to October 4, 2022. Only observational studies investigating the prevalence of SB in individuals with chronic pain were included. The methodological quality of the included studies was assessed using the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement. A meta-analysis was conducted to quantify the prevalence of SB in the population, and the command “Metaprop” was used in STATA/MP 16. In addition, factors explaining the association between chronic pain and SB identified through regression analysis were investigated.

Results

A total of 19 studies were included in this review (N = 3,312,343). The pooled lifetime prevalence of SI and SA was 28.90% (95% confidence interval, 17.95 to 41.26%) and 10.83% (5.72 to 17.30%), respectively, in a mixed sample comprising various chronic pain conditions. Importantly, the pooled prevalence of past 2-week SI was as high as 25.87% (18.09 to 34.50%). The methodological quality of the included studies was not optimal, and studies using validated SB assessment tools were lacking. Potential protective factors against SB in this population included pain coping and self-efficacy, older age, certain race/ethnicity groups, and marriage.

Conclusion

This systematic review and meta-analysis demonstrated the high prevalence of SB in individuals with chronic pain. Specifically, around 1 in 4 individuals with chronic pain had SI within the last 2 weeks. However, there was considerable heterogeneity in the pooled prevalence of SB in this population.
Postdoctoral researchers' perspectives on working conditions and equal opportunities in German academia
Authors: Davidson Jacob D. , de Oliveira Lopes Felipe Nathan , Safaei Sajjad , Hillemann Friederike , Russell Nicholas J. , Schaare H. Lina
Published: September 2023

Postdoctoral researchers (postdocs) are an essential component of the scientific workforce in German universities and research institutions and play a vital role in advancing knowledge and innovation. However, the experiences of postdocs and other early career researchers (ECRs) indicate that working conditions pose a significant challenge to the pursuit of a long-term research career in Germany—particularly for international scientists and those from marginalized groups. We examine how unstable working conditions as well as insufficient structural support for equal opportunities and diversity are significant obstacles for the career development of ECRs in German academia. We discuss these issues with the aid of an extensive survey recently conducted and published by PostdocNet, a target-group network representing the interests of postdocs across Germany's Max Planck Society. The survey drew responses from 659 postdoctoral researchers working at the Max Planck Society and represents one of the few datasets of postdoctoral researchers' perspectives in Germany. Building on these findings, we suggest actions at governmental, institutional, and individual levels to improve the working conditions of postdoctoral researchers in Germany.
Case Report: A playful digital-analogical rehabilitative intervention to enhance working memory capacity and executive functions in a pre-school child with autism
Authors: Panesi Sabrina , Dotti Marina , Ferlino Lucia
Published: September 2023

Background

Autism Spectrum Disorder (ASD) is often associated with deficits in Working Memory Capacity (WMC) and Executive Functions (EFs), as early as the first years of life. Research has shown that, even young children with ASD, WMC and EF deficits can be effectively addressed through interventions employing digital and/or analogical tools. Early intervention is important because executive dysfunction can negatively impact on the quality of life, both of children and their families. However, very few studies have been carried out involving intervention with pre-schoolers with ASD. To fill this gap, we developed an intervention that promotes pre-schoolers’ WMC and EFs by employing both digital apps and analogical playful activities. This study reports on the feasibility of this intervention, which was carried out in a rehabilitative context.

Methods

A male pre-schooler diagnosed with ASD was engaged in a total of 17 intervention sessions, all held in a clinical context, over a nine-week period. Outcomes were measured using a battery of pre- and post-treatment tasks focusing on WMC, EFs and receptive language. The clinician who administered the intervention made written observations and noted any improvements in the child’s performance emerging from the digital and analogical activities.

Results

The pre- and post-test scores for the cognitive tasks revealed qualitative improvements in the following cognitive domains: (a) WMC in the language receptive domain; (b) updating in WMC; (c) inhibition, specifically concerning control of motor response; (d) receptive vocabulary. Furthermore, when monitoring the child’s performance, the clinician noted improvement in almost all the playful activities. Particularly notable improvements were observed in interaction with the apps, which the child appeared to find very motivating.

Conclusion

This study supports feasibility of a playful digital-analogical intervention conducted by a clinician in a rehabilitation context to promote cognitive abilities in pre-schoolers with ASD. Further studies are needed to establish whether the intervention’s effectiveness can be generalized to a broad sample of children with ASD.
An innovative state-of-the-art health storytelling technique for better management of type 2 diabetes
Authors: Ali Sumbal , Bakht Shumaila , Ullah Jan Atta , Alam Iftikhar , Almajwal Ali Madi , Osaili Tareq , Obaid Reyad Shakir , Faris MoezAllslam Ezzat , Cheikh Ismail Leila , Najah Farah , Radwan Hadia , Hasan Haydar , Hashim Mona , AlBlooshi Sharifa , Sehar Bismillah , Zeb Falak
Published: September 2023

Background

Type 2 diabetes (T2D) is a chronic lifelong disease that requires long-term prevention and management strategies in a community setting. A health story is a novel technique that may be used as an effective tool for better prevention and management of T2D.

Objective

The main objective of this study is to develop a story to be used as a social health technique based on contemporary scientific knowledge that may be used at a community level for better communication and management of T2D.

Methods

A community–academic partnership was formed with a not-for-profit Nutrition Education, Awareness, and Training (NEAT) organization in Khyber Pakhtunkhwa, Pakistan. We agreed to develop a story that may be used as a health and nutrition education communication tool for better management of patients with T2D. The following phases were followed during the story creation process: (1) the theory phase, (2) the modeling phase, and (3) the evaluation phase. Raters evaluated the story to determine its literary and scientific quality, comprehensiveness, and T2D specificity.

Results

The title of the story translated into English is “The Story of Diabetes—The Story of Success.” It is text based and contains 86 pages in the local language, “Pashto,” with an English translation. The story is divided into five chapters and describes the initial diagnosis, fear associated with the disease, issues related to referral to certified practitioners, the importance of a balanced diet, and related lifestyle habits. After story evaluation, the raters suggested its literary and scientific quality, comprehensiveness, and T2D specificity (Pearson correlation scores of >0.8).

Conclusion

This unique story was created for T2D and found to be of significant quality in terms of its literary and scientific quality, as well as its comprehensiveness and diabetes specificity. As a result, it may be suggested that it can be used in subsequent studies to improve T2D management among adult patients.
Adaption and validation of simplified Chinese version of the Low Back Pain Knowledge questionnaire (sC-LKQ)
Authors: Wang Zhe , Xie Yinyao , Dózsa-Juhász Olívia , Makai Alexandra , Járomi Melinda
Published: September 2023

Introduction

Low back pain (LBP) knowledge questionnaire (LKQ) was developed by a Brazilian research group in 2009. It has been cross-culturally adapted to many languages with good reliability and validity. This work aimed to translate and validate the LKQ into a simplified Chinese version and to evaluate the self-efficacy in LBP among Chinese participants from China and Hungary.

Methods

A total of 431 people participated in this research, which lasted from September 2021 to June 2022 and was conducted on the Credamo online platform. The simplified Chinese LKQ (sC-LKQ) was generated through translation and cross-cultural adaptation guidelines. The participants were selected to fill out demographic questions, the sC-LKQ, and the Roland-Morris Disability Questionnaire (RMDQ). The reliability and validity of the data were evaluated using SPSS 28.0.

Results

The sC-LKQ showed good internal consistency (Cronbach’s alpha was 0.79), and the intraclass correlation value was 0.85. There were five components in the questionnaire with good construct validity. The scores of RMDQ had negatively correlated with sC-LKQ.

Conclusion

In the Chinese population, the sC-LKQ demonstrated excellent psychometric qualities and could be used to evaluate self-efficacy in clinical practice and research.
A panoramic view of personalization based on individual differences in persuasive and behavior change interventions
Authors: Alslaity Alaa , Chan Gerry , Orji Rita
Published: September 2023

Persuasive technologies are designed to change human behavior or attitude using various persuasive strategies. Recent years have witnessed increasing evidence of the need to personalize and adapt persuasive interventions to various users and contextual factors because a persuasive strategy that works for one individual may rather demotivate others. As a result, several research studies have been conducted to investigate how to effectively personalize persuasive technologies. As research in this direction is gaining increasing attention, it becomes essential to conduct a systematic review to provide an overview of the current trends, challenges, approaches used for developing personalized persuasive technologies, and opportunities for future research in the area. To fill this need, we investigate approaches to personalize persuasive interventions by understanding user-related factors considered when personalizing persuasive technologies. Particularly, we conducted a systematic review of 72 research published in the last ten years in personalized and adaptive persuasive systems. The reviewed papers were evaluated based on different aspects, including metadata (e.g., year of publication and venue), technology, personalization dimension, personalization approaches, target outcome, individual differences, theories and scales, and evaluation approaches. Our results show (1) increased attention toward personalizing persuasive interventions, (2) personality trait is the most popular dimension of individual differences considered by existing research when tailoring their persuasive and behavior change systems, (3) students are among the most commonly targeted audience, and (4) education, health, and physical activity are the most considered domains in the surveyed papers. Based on our results, the paper provides insights and prospective future research directions.
Factors associated with citations of articles on circular economy in the Web of Science: modeling for main publishers
Authors: Minchón-Medina Carlos Alberto , Timaná-Palacios Daphne Jannet , Alvarez-Risco Aldo , Del-Aguila-Arcentales Shyla , Yáñez Jaime A.
Published: September 2023

Introduction

The publication of articles on the circular economy has different associated factors to explain the citations registered in the Web of Science.

Method

Articles from the publishers Elsevier, MDPI, Taylor & Francis, Wiley, and Springer Nature were evaluated.

Results

It was expected that the older the article was, the more citations it had received, but this was not always the case. It was also recognized that there was a lower number of citations if the articles were too large or if they had too many references.

Discussion

This analysis helps to establish the factors that must be addressed in order to publish in journals that have a high citation rate. Conclusion: Based on speci?c articles and with speci?c references, it will be possible to increase the probability of citations.
Detecting and identifying the reasons for deleted tweets before they are posted
Authors: Mubarak Hamdy , Abdaljalil Samir , Nassar Azza , Alam Firoj
Published: September 2023

Social media platforms empower us in several ways, from information dissemination to consumption. While these platforms are useful in promoting citizen journalism, public awareness, etc., they have misuse potential. Malicious users use them to disseminate hate speech, offensive content, rumor, etc. to promote social and political agendas or to harm individuals, entities, and organizations. Oftentimes, general users unconsciously share information without verifying it or unintentionally post harmful messages. Some of such content often gets deleted either by the platform due to the violation of terms and policies or by users themselves for different reasons, e.g., regret. There is a wide range of studies in characterizing, understanding, and predicting deleted content. However, studies that aim to identify the fine-grained reasons (e.g., posts are offensive, hate speech, or no identifiable reason) behind deleted content are limited. In this study, we address an existing gap by identifying and categorizing deleted tweets, especially within the Arabic context. We label them based on fine-grained disinformation categories. We have curated a dataset of 40K tweets, annotated with both coarse and fine-grained labels. Following this, we designed models to predict the likelihood of tweets being deleted and to identify the potential reasons for their deletion. Our experiments, conducted using a variety of classic and transformer models, indicate that performance surpasses the majority baseline (e.g., 25% absolute improvement for fine-grained labels). We believe that such models can assist in moderating social media posts even before they are published.
The road from manual to automatic semantic indexing of biomedical literature: a 10 years journey
Authors: Krithara Anastasia , Mork James G. , Nentidis Anastasios , Paliouras Georgios
Published: September 2023

Biomedical experts are facing challenges in keeping up with the vast amount of biomedical knowledge published daily. With millions of citations added to databases like MEDLINE/PubMed each year, efficiently accessing relevant information becomes crucial. Traditional term-based searches may lead to irrelevant or missed documents due to homonyms, synonyms, abbreviations, or term mismatch. To address this, semantic search approaches employing predefined concepts with associated synonyms and relations have been used to expand query terms and improve information retrieval. The National Library of Medicine (NLM) plays a significant role in this area, indexing citations in the MEDLINE database with topic descriptors from the Medical Subject Headings (MeSH) thesaurus, enabling advanced semantic search strategies to retrieve relevant citations, despite synonymy, and polysemy of biomedical terms. Over time, advancements in semantic indexing have been made, with Machine Learning facilitating the transition from manual to automatic semantic indexing in the biomedical literature. The paper highlights the journey of this transition, starting with manual semantic indexing and the initial efforts toward automatic indexing. The BioASQ challenge has served as a catalyst in revolutionizing the domain of semantic indexing, further pushing the boundaries of efficient knowledge retrieval in the biomedical field.
Ruminal bacterial communities differ in early-lactation dairy cows with differing risk of ruminal acidosis
Authors: Golder Helen Marie , Rehberger Josh , Smith Alexandra Helena , Block Elliot , Lean Ian John
Published: September 2023

Introduction

Early-lactation Holstein cows (n= 261) from 32 herds in three regions (Australia, California, and Canada) were previously categorized using a discriminant analysis model as being at a high (26.1% of cows), medium (26.8% of cows), or low risk (47.1% of cows) of ruminal acidosis. We aimed to investigate if (1) risk of acidosis would be associated with ruminal bacterial taxa and dietary nutrient components, (2) there would be individual or combinations of bacterial taxa associated with acidosis-risk groups, and (3) the abundance of bacterial taxa would be associated with the intake of dietary nutrient components.

Methods

Diets ranged from pasture supplemented with concentrates to total mixed rations. Bacteria 16S ribosomal DNA sequences from rumen samples collected < 3 hours after feeding via stomach tube were analyzed to determine bacterial presence. The relative abundance of each bacterial phylum and family was center log transformed and the transformed family data were subjected to two redundancy analysis biplots, one for acidosis risk group and one for region, to identify the 20 best-fit bacterial families from each respective redundancy analysis. A total of 29 unique families were identified when the lists of 20 families were combined from each redundancy analysis, and these 29 families were termed "influential" families." The association of acidosis-risk groups with the abundance of individual influential families was assessed by mixed models. Backward stepwise elimination mixed models were used to determine the bacterial taxa associated with each acidosis-risk group and the dietary nutrients associated with the abundance of the bacterial taxa.

Results and discussion

High-risk acidosis cows were associated with increased abundances of Anaerocella_f and Veillonellaceae and decreased abundances of several bacterial families with different characteristics. Five phyla: Firmicutes [odds ratio (OR) = 7.47 ± 7.43], Spirochaetes (OR = 1.28 ± 0.14), Lentisphaerae (OR = 0.70 ± 0.07), Planctomycetes (OR = 0.70 ± 0.09), and Tenericutes (OR = 0.44 ± 0.15), and nine families were associated with a higher risk of acidosis. Of the nine phyla identified to be of interest based on abundance and strength of association with acidosis-risk groups, all had one or more dietary nutrient that predicted their abundance. Sugar was the most frequently associated nutrient with the nine phyla, and was present in 78% (seven out of nine phyla) of the models; crude protein was present in 56% of models and crude fat was present in 44% of the models. Sugar and crude protein were most associated with the influential families and all but three families had one or more nutrient predictive of their abundance. Ruminal bacterial taxa are associated with ruminal acidosis; dietary sugar and crude protein are vital predictors of these and, thus, of ruminal acidosis risk.
Data for decision-making for sustainable food systems transformation in the Eastern Cape of South Africa: what is needed?
Authors: Karan Romyne , Mowlds Sinead , McKeown Peter C. , Lundy Mark , Spillane Charles
Published: September 2023

Transforming global food systems to promote food and nutritional security can help alleviate both poverty and ill-health, and support sustainable development. Such transformations need to be tailored and sensitive to the vulnerabilities and needs of marginalized communities yet are hindered by knowledge gaps. In particular, the food and nutrition security needs of the most vulnerable are often poorly understood, as are the policy frameworks and resource requirements associated with meeting their needs. To aid the development of frameworks to address these gaps, we review the current state of indicators relating to food systems for the improvement of food and nutrition security at the national and sub-national levels in South Africa. We identify gaps in the decision-making data in South Africa, including absence of food and nutrition security indicators. Integration of the South African Multidimensional Poverty Index and Food System Dashboard indicators could help address this, especially if applied at a sub-national level. Participatory food system mapping could also link data collection with system-level interactions and feedback loops to inform sub-national stakeholders in achieving food and nutritional security.
Bovine infectious abortion: a systematic review and meta-analysis
Authors: Hecker Yanina Paola , González-Ortega Sara , Cano Santiago , Ortega-Mora Luis Miguel , Horcajo Pilar
Published: September 2023

The aim of the present systematic review and meta-analysis was to identify the main infectious agents related to bovine abortion worldwide in the period between 2000 and 2022. First, we investigated the global prevalence of infectious agents related to bovine abortion. For this analysis, only 27 articles detected of a wide panel of agents were included. The random effects model revealed that the estimated prevalence of the abortifacient agents in bovine abortion was 45.7%. The heterogeneity among studies was high, but Egger’s test showed that there was no publication bias, even though the total number of samples analyzed in these articles was variable. There was no significant effect of the year of the study publication on the estimated prevalence, although an increasing trend was observed over time, possibly due to the implementation of new diagnostic techniques. Then, we analyzed the prevalence of the main transmissible agents in bovine abortion. For this analysis, 76 studies that analyzed 19,070 cases were included. Some infectious agent was detected in 7,319 specimens, and a final diagnosis was reached in 3,977 of these, when both the infectious agent and compatible histopathological changes were detected. We found that Neospora caninum was the most detected agent (22.2%), followed by opportunistic bacteria (21.4%), Chlamydiaceae family (10.9%) and Coxiella burnetii (9.5%). Regarding viral agents, bovine herpes virus type 1 and bovine viral diarrhea displayed similar prevalence rates (approximately 5%). After considering the description of specific histopathological changes, our analyzes showed that N. caninum was a confirmed cause of abortion in 16.7% of the analyzed cases, followed by opportunistic bacteria (12.6%) and Chlamydia spp. (6.8%); however, C. burnetii was only confirmed as a cause of abortion in 1.1% of the cases. For all agents, the heterogeneity among studies was high, and the subgroup analyzes discarded the diagnostic method as the cause of such heterogeneity. This study provides knowledge about the global prevalence of the different infectious agents related to bovine abortion, the most coming of which is N. caninum. In addition, this review reveals the existing deficiencies in the diagnosis of bovine abortion that must be addressed in the future.
Phylogenetic analysis of rabies surveillance samples from north and northeast Brazil
Authors: Cunha Tânia Cristina Alves da Silveira da , Silva Fábio Silva da , Silva Sandro Patroca da , Ribeiro Cruz Ana Cecília , Paiva Francisco Amilton dos Santos , Casseb Livia Medeiros Neves , Nascimento Ana de Nazaré Silva do , Oliveira Iza Alencar Sampaio de , Branco Marlon de Araújo Castelo , Oliveira Rodrigo Adolpho Brasil de , Durans Darlene de Brito Simith , Paz Thito Yan Bezerra da , Coelho Taciana Fernandes Souza Barbosa
Published: September 2023

Viruses of the Lyssavirus genus are classified into several genotypes (GT1 to GT7), of which only GT1 (classic rabies virus—RABV) has a cosmopolitan distribution and circulates in Brazil. GT1 is subdivided into several antigenic variants (AgV) maintained in independent cycles with a narrow host range and distinct geographic distributions, namely, AgV1 and AgV2 found in dogs, AgV3 in the vampire bats Desmodus rotundus, and AgV4 and AgV6 in bats non-hematophagous Tadarida brasiliensis and Lasiurus cinereus, a common variant of marmoset (Callithrix jacchus), and crab-eating fox (Cerdocyon thous). In this study, we performed phylogenetic analysis to identify at the antigenic variant level; six RABV genomes derived from the Rabies Surveillance in the north and northeast regions of Brazil. The analysis resulted in the formation of 11 monophyletic clusters, each corresponding to a particular variant, with high bootstrap support values. The samples were positioned inside the AgV3, AgV6, and Callithrix variant clades. This is the first report of the AgV6 variant found in northern Brazil, which provides valuable information for rabies surveillance in the country. The possibility of viral spillover has been much debated, as it deals with the risk of shifting transmission from a primary to a secondary host. However, more genomic surveillance studies should be performed, with a greater number and diversity of samples to better understand the transmission dynamics of each variant to detect changes in its geographic distribution and spillover events.
Case report: Blindness associated with Learedius learedi trematode infection in a green sea turtle, Chelonia mydas, of the northern Red Sea
Authors: Morick Danny , Bachmann Vanessa M. , Shemesh Eli , Botero-Anug Ana Maria , Zemach-Shamir Ziv , Aizenberg Zahi , Davidovich Nadav , Goldberg Daphne W. , Kan Gaston , Ofri Ron , Tchernov Dan , Levy Yaniv
Published: September 2023

Spirorchiid blood flukes are widespread in sea turtles, causing disease and mortality in their populations, with high prevalence in several ocean basins. Besides being leading parasitic causes of sea turtle strandings in several parts of the world, these infectious agents can cause endocarditis, vasculitis, thrombosis, miliary egg granulomas, and aneurysms, which ultimately may compromise the survival of green sea turtles. More severe cases may also result in multifocal granulomatous meningitis or pneumonia, both of which can be fatal. Herein, we report the first case of severe trematode infection, Caused by Learedius learedi, in a green sea turtle in the northern Red Sea; this infection is associated with bilateral blindness. Necropsy revealed multiple granulomas with intralesional trematode eggs in the optic nerve, eyes, spleen, heart, and lungs. The parasite was identified as Learedius learedi through specific primers of the ribosomal genome and COI sequences obtained from GenBank. Altogether, these findings emphasize the importance of recognizing the systemic nature of this particular fluke infection to ultimately protect the lives of these marine animals and ensure the sustainability of these species in the wild.
Distinguishing Hybrids of Golden Thailand and Malaysian Strains of Climbing Perch, Anabas testudineus (Bloch, 1792), Using Multivariate Analyses of Morpho-Meristic Traits
Authors:   Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia, DASUKI AWAWU , ESA YUZINE B. , CHRISTIANUS ANNIE , ISMAIL MOHAMMAD FADHIL SYUKRI , OGAH SAMUEL IJABO
Published: September 2023

The climbing perch, Anabas testudineus (Bloch, 1792), is a commercially important freshwater fish in Southeast Asia. To meet demand, establishing stock development breeding programmes is essential. However, there is a lack of scientific literature on parent-hybrids differences. This study aims to use morphometric characteristics to identify important predictors and determine their heritability to address the knowledge gap. Two strains of A. testudineus and their corresponding hybrid groups, represented by T1–T4 (T1: golden Thai (♀) × golden Thai (♂), T2: golden Thai (♀) × Malaysian (♂), T3: Malaysian (♀) × Malaysian (♂) and T4: Malaysian (♀) × golden Thai (♂)) were used for the experiment. Thirty individuals of each strain were randomly selected from their respective tanks for morphometric characteristics assessment. Twenty-five morphometric measurements were taken, and adjustments were made for body size effects. These measurements were then subjected to multivariate analysis with a 5 % selection intensity for genetic advancement, focusing on body depth and base of anal fin length. The morphometric characters, including postorbital length, lowest body depth,the base of analfin length,total length, standard length, pre-pectoral fin length,the base of pectoralfin length, and base of pelvic fin length based on the canonical coefficient, were identified as distinguishing factors between hybrids and the parental population. The study showed that body length heritability was greater than 60 %, indicating a significant additive genetic effect that surpasses the impact of the environmental effect and thus could be used as a potential characteristic for selective breeding to improve the desired trait.
Síndrome de Bloom: uma abordagem diagnóstica, evolução clínica e revisão
Authors: Nascimento Maria Paula Junqueira , Tavares Milena Fonseca Prado , Rodovalho Bárbara , Cavalcante Gabriela Ferreira , Emerici Giovanna Marques , Silva Maria Eugênia Carbonaro , Nagem Ana Luiza Ledro , Gomes Iara Lafetá , Dos Santos Ludmilla Simeão Ferreira , Ferreira Edson Marques Gomes , Côrtes Gabriel Augusto Silva
Published: September 2023

Introdução: A Síndrome de Bloom, também conhecida como síndrome de Bloom-Torre-Machacek, é uma doença genética rara e hereditária, classificada como distúrbio de instabilidade cromossômica. Caracterizada por danos frequentes aos cromossomos, essa síndrome apresenta uma ampla variedade de manifestações clínicas que podem afetar múltiplos sistemas do corpo. Apresentação do Caso: Um garoto de 9 anos, residente do estado de Goiás, foi levado por seus pais atenciosos a uma consulta pediátrica devido a preocupações com seu crescimento subótimo, frequentes erupções cutâneas e notável sensibilidade à luz. Carlos nasceu de uma gravidez sem complicações, e seu desenvolvimento inicial foi típico. Ele é o filho mais novo de dois irmãos, e não há um histórico familiar conhecido de doenças genéticas. Discussão: Nesta síndrome (de Bloom), é imprescindível serem  abordados aspectos essenciais da doença, incluindo sua epidemiologia, etiologia genética e herança autossômica recessiva. Serão analisados critérios diagnósticos atualmente aceitos, considerando os achados clínicos mais comuns, como crescimento comprometido, fotossensibilidade, predisposição a infecções, anormalidades dermatológicas, risco aumentado de câncer e problemas respiratórios. Uma abordagem multidisciplinar para o diagnóstico precoce e o tratamento adequado será discutida, destacando a importância de testes genéticos, aconselhamento genético e acompanhamento médico regular para pacientes com Síndrome de Bloom. Além disso, serão abordadas as opções de tratamento disponíveis, focadas no manejo dos sintomas e na prevenção de complicações, bem como a importância do apoio psicológico e terapêutico para melhorar a qualidade de vida dos pacientes. Conclusão: Portanto, é fundamental promover o entendimento da doença, facilitando a identificação e a intervenção precoce,  para melhorar o prognóstico e o bem-estar dos indivíduos afetados por essa rara e complexa síndrome genética.
Síndrome de Korsakoff: uma abordagem diagnóstica, evolução clínica e revisão
Authors: Corrêa Rone Passos Vasconcelos , Ferreira Caio César , Jubé Isabella Machado Fleury , Luz Lauren Soares , Santos Thaís Brasil , Torres Lorena Da Silva , De Andrade Islla Giovanna Melo , Do Nascimento Natália Rodrigues , Siqueira Arthur de Souza , Guizzetti Maria Isabel Araujo , Do Carmo Adriani Ferreira , Flávio Gabriela Alves Louzada , Peron Filho Joel Neves , De Paula Mirla Mirtes Mastub
Published: September 2023

Introdução: A Síndrome de Korsakoff (SK) é uma perturbação neurológica crônica multifatorial, na qual os danos cerebrais em conexões dos circuitos fronto-cerebelar e límbico geram: amnésia anterógrada e confabulação, alteração do estado de consciência, marcha atáxica, oftalmoparesia e nistagmo. Apresentação do Caso: Um homem de 47 anos, nascido em Goiás, foi levado ao pronto-socorro por membros da família devido a um estado mental alterado caracterizado por lapsos de atenção, discurso confuso, oftalmoplegia e instabilidade ao caminhar. O paciente tinha um histórico de consumo prolongado de álcool, começando aos 21 anos, com um aumento notável no consumo de álcool destilado nos últimos 18 meses. Ele havia tentado tratamentos de reabilitação para desintoxicação várias vezes, mas sem sucesso duradouro. Discussão: O diagnóstico da síndrome de Korsakoff é eminentemente clínico, podendo a ressonância magnética cerebral ser útil para confirmar a suspeita clínica, apesar de não apresentar achados e locais de lesão patognomônica a síndrome, podem ser evidenciados alterações macroscópicas nos corpos mamilares e em casos crônicos, presença de gliose. Os exames de imagem podem apresentar lesões na porção medial do tálamo e mesencéfalo, enquanto que imagens de tomografia computadorizada podem apresentar dilatação do terceiro ventrículo e ventrículos laterais nos pacientes com síndrome de Korsakoff. Conclusão: É necessário a realização de estudos contínuos sob tal síndrome a fim de otimizar o diagnóstico e os cuidados no tratamento.