bluuebunny/crossref_metadata_2025 huggingface dataset:
Location-Based Health Service Information System Design (Case Study of Sidoarjo District Health Services)
Authors: Ardiansyah Wiwit , Kautsar Irwan Alnarus , Taurusta Cindy
Published: October 2022

In this day and age, humans need more efficient technology to facilitate activities in various aspects of life. Health is one of the important aspects of life to ensure human survival. To ensure the quality of public health, it is necessary to have a health service center. Sidoarjo City is one of the areas that has a lot of health services both from the government and from the private sector. Sidoarjo is one of the cities in East Java which is located south of the city of Surabaya. As an effort to promote better health services, a media is needed to make it easier for the public to get information on the location of health services in the Sidoarjo City area. With the current covid-19 pandemic, the author has developed a Web service for a location-based health service information system, where the Web will be designed using the Python programming language and using the Flask framework with output displaying data and the location of hospitals and polyclinics in Sidoarjo City. This system is designed to make it easier for the public to find the nearest service location in Sidoarjo City.
High Rate Slim ESP Viability Assessment in the Field
Authors: Alshinaiber Fahad , Windiarto Yhossie , Alhashem Mayadah , Pessoa Rodrigues Rui
Published: October 2022

Producing oil at full potential with an electrical submersible pump (ESP) in a slim well remains a challenge in the petroleum industry. A conventional slim ESP system is limited in produced oil delivery rate with an associated risk of damaging the motor lead extension (MLE) of the ESP during the running in hole due to tight clearance. Finding a high rate new slim ESP technology is crucial to enable production of wells at full potential and eliminate slot recovery. This paper shares the success stories of testing two high rate slim ESP installations, and provides the advantages and disadvantages for each of the two approaches for high rate slim ESP design.

The high rate slim ESP design options were assessed through field trials collaborating with different manufacturers. The slim ESP viability evaluation metrics were maximum rates, ESP performances, completion installation simplicity and surface controller compatibility. These metrics ensure eliminating unnecessary costs of ESP replacement in the field. The first design option is an inverted ESP design with an induction motor installed at the top. Such a design is advantageous in allowing a pump with a bigger outer diameter (OD), eliminating MLE installation on the pump housing due to an increased clearance, and consequently achieving a higher rate. The second design option uses a permanent magnetic motor (PMM) in a standard ESP configuration. The second design with the smaller ESP OD allows high motor speed, thus providing a higher head capacity and higher flow rate. A modification in the variable speed drive (VSD) design is required to enable controlling the PMM.

The field trial result revealed multiple benefits of the high rate slim ESP systems in the field. The high-speed system that utilizes PMM retains the standard ESP configuration, which simplifies well completion and avoids installation complexity. The reduced ESP OD provides more clearance in the well, leading to minimized possible MLE damage during running in hole. Although the VSD modification is possible, the PMM maximum speed will be limited by the maximum capacity of the existing transformer installed in the platform. Finally, both systems lead to a significant cost avoidance by eliminating the need for slot recovery of the produced well at full potential in a slim-well, and avoiding unnecessary replacement of ESP surface equipment. The two systems were already successfully evaluated with a continuous run for more than 365 days without any issues.
A Study on the Legal Limits of Using Attachable GPS Tagging Systems in Police High-Speed Pursuit Situations
Authors:   Korean Association of Public Safety and Criminal Justice, Lee Hoon
Published: October 2022

Recently, the Korean National Police Agency in conjunction with Ministry of Science and ICT has initiated a research and development project on attachable GPS tagging systems that allow its officers to tag, track, and arrest fleeing suspects by reducing the necessity to engage in a hazardous high-speed pursuit. The new systems that launch a GPS bullet at a fleeing or about to flee vehicle collect the location information of the vehicle without a search warrant until police officers successfully track and stop the vehicle. There is no denial that police use of GPS tracking technology during the conventional investigation process requires a warrant issued by courts. As police officers who use GPS tracking devices during high-speed chases have only a limited ability to write a warrant, the legal limits of the police GPS tracking systems must be carefully established. Furthermore, the research and development project also needs to consider safety rules as the GPS device may cause bodily harms to suspects, bystanders as wells as pursuing police officers. In this regard, the present study attempted to establish police manuals of GPS tracking systems during high-speed pursuit situations within the context of the Fourth Amendment by analyzing StarChase guidelines of the 14 police departments in the United States. Upon analysis, the current study proposed that in order to apply the GPS device in the field police officers must have the equivalent of probable cause of committing a crime. In case of misdemeanor, use of the GPS device must be restricted unless the fleeing suspect vehicle endangers police officers and other citizens, an arrest warrant is issued for the suspect, and the crime that leads to police pursuit is a felon of minimum sentence of three years in prison. With respect to safety considerations, the current study discussed the safe approachability to a fleeing vehicle within targeting range, weather conditions, and suspect vehicle weaving. Finally, the present study proposed a training requirement that police officers must complete before applying the GPS tracking device.
A Study on the Effects of Friend Relationships and Parental Rearing Attitudes Perceived by Middle School Students on Their Aggression
Authors:   Korean Association of Public Safety and Criminal Justice, Hwang Jong-yong
Published: October 2022

The purpose of this study is to find out the effect of friend relationships and parental rearing attitudes perceived by middle school students on their aggression through a multiple regression model and to find countermeasures based on them. To this end, this study used data from 2,384 freshmen in middle schools in the Korean Children and Youth Panel Survey (KCYPS) 2018 conducted by the National Youth Policy Institute. The research data were analyzed using SPSS 24.
As a result of the analysis, between friend relationships, negative friend relationships had a significant effect on the increase in aggression, and among parental rearing attitudes, parental inconsistency and rejection had a significant effect on the increase in aggression, and parental autonomy support and warmth had a significant effect on the decrease in aggression. Comparing the size of effects, it was found that negative friend relationships were larger than parental rearing attitude variables, and it is not irrelevant to the tendency of middle school students to value the formation and maintenance of relationships with friends more than parents.
Based on the above research results, it was argued that in order to reduce the aggression of middle school students to prevent school violence, a program to resolve negative relationships with friends and improve parental rearing attitudes is urgently needed, and to this end, the operation of the program should be substantiated by improving the budget payment method. In addition, it's been more than a year since the autonomous police system was launched, it was also suggested to conduct restorative justice in the police stages as a member of the local community in cooperation with local governments and community youth organizations.
HIT-scISOseq: High-throughput and High-accuracy Single-cell Full-length Isoform Sequencing
Authors: Shi Zhuoxing , Chen Zhi-Chao , Zhong Jia-Yong , Hu Kun-Hua , Zheng Yingfeng , Chen Ying , xie Shang-Qian , Bo Xiaochen , Luo Feng , Tang Chong , Xiao Chuan-Le , Liu Yi-Zhi
Published: October 2022

Although long-read single-cell isoform sequencing (scISO-Seq) can reveal transcriptomic dynamics in individual cells invisible to NGS-based single-cell RNA analysis, scISO-Seq has been limited by low throughput, high error rates, and long running time.Here, we introduce HIT-scISOseq, the first method that concatenates multiple full-length cDNAs for PacBio circular consensus sequencing (CCS) sequencing to achievehigh-throughput, and high-accuracy single-cell isoform sequencing. HIT-scISOseq can yield >10 million high-accuracy full-length isoforms in a single PacBio Sequel II SMRT Cell 8M. We have developed scISA-Tools that demultiplex HIT-scISOseq concatenated reads into single-cell full-length isoforms with >99.99% accuracy and specificity. We have applied HIT-scISOseq to characterize the transcriptome of thousands of corneal limbus cells, and reveal cell-type-specific isoform expression changes that are previously not identified by NGS-based scRNAseq. HIT-scISOseq is a high-throughput, high-accuracy, and technically accessible method that can be used by most laboratories to accelerate the burgeoning field of long-read single-cell transcriptomics.
Discrete-time constrained discounted stochastic games with an uncountable state space
Authors: Zhang Wenzhao , Liu Congying , Zou Xiaolong
Published: October 2022

In this paper, we consider nonzero-sum discrete-time constrained stochastic games with the expected discounted cost criteria and an uncountable state space. The cost functions are allowed to be unbounded from below and from above. First, we construct the auxiliary game models and the equivalent countable-state game models. Then, we formulate the best response linear programs and duality programs related to the game models, and undertake the duality analysis via a technique of occupation measures. By introducing the weak norm topology on the set of all stationary strategies, we establish the compactness and metrizability of the set of all stationary strategies and occupation measures. Finally, by the combination of countable-state approximation and duality analysis, we show the extension of Nash equilibria of the countable-state game models are ε-equilibria when the discretization of state space is suitable.
Lower ultra-short-term heart rate variability is a risk factor for worse mucosal healing in ulcerative colitis
Authors: Guo Jianan , Chen Wenguo , Zhu Huatuo , Chen Hongtan , Teng Xiaodong , Xu Guoqiang
Published: October 2022

Background
Psychological stress has been proved to be a risk factor for exacerbation for ulcerative colitis (UC). However, traditional approaches of quantifying psychological stress using psychological scales are time-consuming and the results may not be comparable among patients with different educational levels and cultural backgrounds. Alternatively, heart rate variability (HRV) is an indicator for psychological stress and not biased by educational and cultural backgrounds.
Aims
In this study, we try to explore the relationship between psychological stress and UC by analyzing the effect of ultra-short-term HRV on mucosal and histological remission status of UC.
Methods
This is a retrospective case-control study on UC inpatients from 2018 through 2020. Ultra-short-term HRV were calculated using baseline electrocardiography. Patients were divided into case and control groups according to their Mayo endoscopic scores or histological Geboes scores. Three variables of ultra-short-term HRV (SDNN, SDSD and RMSSD) were compared between different groups. And for those variables with significant differences, we built univariate and multivariate logistic regressions to depict the relationship between HRV variables and remission status of UC.
Results
All three HRV variables showed significant differences between the mucosal groups. However, none of them showed significant difference between the histological groups. In further logistic regression analyses, smaller RMSSD was detected as a risk factor for severe mucosal healing status (OR = 5.21).
Conclusions
Lower ultra-short-term HRV (i.e. smaller RMSSD) is shown to correlate with worse mucosal healing status. However, ultra-short-term HRV cannot predict histological healing status according to our data.
Surface Roughness Analysis in Finishing End Milling of Hardox® 450 Steel Using Multilayer Graphene-based Nanofluid
Authors: Passari Émerson S. , Souza André J. , Vilanova Abdiel M.
Published: October 2022

Hardox® 450 is a structural steel commonly used in the mining and agricultural sectors due to its hardness and toughness combined with high abrasion wear resistance. It is widely known that a good surface quality minimizes the occurrence of cracks generating higher fatigue resistance. However, the characteristics of this steel make it difficult to cut, resulting in greater complexity in the choice of process parameters. Additionally, the general industry concern for clean machining encourages the use of lubricooling conditions with less environmental impact. In order to address these difficulties, the paper aims to investigate the performance of multilayer graphene-based nanofluid applied in reduced quantity (NF-RQL) on surface roughness generated by finishing end milling of Hardox® 450 when compared to dry and flood machining. The cutting parameters at three levels were combined, randomized, and analyzed via Box-Behnken Design. The experimental results showed that the lowest roughness values were obtained with NF-RQL, followed by flood machining. The Abbott-Firestone and Amplitude Distribution statistical analysis indicated a greater uniformity of peaks and valleys in the roughness profile obtained by NF-RQL milling than in the other lubricooling environments. All prediction models demonstrated excellent ability to estimate the roughness values. The levels of cutting parameters obtained from the multivariate optimization generated similar roughness values for NF-RQL and flood conditions. However, the material removal rate with the first condition is about 83% higher, justifying its better performance.
Characterization of nitrated polycyclic aromatic hydrocarbons in secondary organic aerosol derived from photooxidation of naphthalene in the presence of NO2
Authors: Zhu Mincong , Huang Mingqiang , Wang Weichao , Shan Xiaobin , Sheng Liusi , Zhao Weixiong , Gu Xuejun , Zhang Weijun
Published: October 2022

Nitrated Polycyclic aromatic hydrocarbons (NPAHs) widely exist in atmospheric aerosol particles are important constituents of brown carbon. Naphthalene and other polycyclic aromatic hydrocarbons (PAHs) are vital precursors of NPAHs. The NPAHs components of secondary organic aerosol (SOA) generated by the photooxidation of naphthalene under different concentrations of NO2 in chamber are on-line measured using synchrotron radiation vacuum ultraviolet photoionization aerosol mass spectrometer (VUV-PIMS) and verified off-line with optical spectrometry in this study. The results demonstrate that NO2 can promote the generation of NPAHs and formation of SOA. Using VUV-PIMS on-line detection, and combined with off-line UV-Vis and infrared spectroscopy verification, it is determined that carboxyls and naphthols are main components of naphthalene SOA particles without NO2, and nitro-naphthalene, dinitro-naphthalene, nitro-naphthol and dinitro-naphthol via the nitration of OH-naphthalene adduct and naphthol products of naphthalene are identified as principal constituents of SOA with NO2. The increase of strong chromophore of N = O of nitro group in NPAHs strengthens the absorbing ability of naphthalene SOA particles. The averaged mass absorption coefficient (< MAC>) over 200–600 nm of naphthalene SOA particles is increases gradually with the increasing concentration of NO2. The < MAC > of naphthalene SOA particles with 1000 ppb NO2 is 688 cm2/g, which is close to to that of biomass burning organic aerosol particles. These results offer the reference for exploring the formation mechanism and optical properties of NPAHs under the background of high concentration of NOx in urban atmosphere.
D-Cycloserine enhances the bidirectional range of NMDAR-dependent hippocampal synaptic plasticity
Authors: Vestring Stefan , Dorner Alexandra , Scholliers Jonas , Ehrenberger Konstantin , Kiss Andreas , Theiss Alice , Rossner Paul , Frase Sibylle , du Vinage Catherine , Wendler Elisabeth , Serchov Tsvetan , Domschke Katharina , Bischofberger Josef , Normann Claus
Published: October 2022

The partial N-methyl-D-aspartate receptor (NMDAR) agonist D-Cycloserine (DCS) has been evaluated for the treatment of a wide variety of psychiatric disorders, including dementia, schizophrenia, depression and for the augmentation of exposure-based psychotherapy. Most if not all of the potential psychiatric applications of DCS target an enhancement or restitution of cognitive functions, learning and memory. Their molecular correlate is long-term synaptic plasticity; and many forms of synaptic plasticity depend on the activation of NMDA receptors. Here, we comprehensively examined the modulation of different forms of synaptic plasticity in the hippocampus by DCS and its mechanism. We found that DCS positively modulates NMDAR-dependent forms of long-term synaptic plasticity (long-term synaptic potentiation, LTP, and long-term synaptic depression, LTD) in hippocampal brain slices of juvenile rats without affecting basal synaptic transmission. DCS binds to the D-serine/glycine binding site of the NMDAR. Pharmacological inhibition of this site prevented the induction of LTP, whereas agonism at the D-serine/glycine binding site augmented LTP and could functionally substitute for decreased amounts of postsynaptically available glutamate. The most probable origin of endogenous D-serine are astrocytes, and its exocytosis is regulated by astrocytic metabotropic glutamate receptors (mGluR1). Functional eradication of astrocytes, inhibition of mGluR1 receptors and their downstream pathways in astrocytes adjacent to postsynaptic neurons prevented the induction of NMDAR-dependent forms of LTP and LTD. Our results support the enhancement of a bidirectional range of NMDAR-dependent hippocampal synaptic plasticity by DCS and D-serine-mediated gliotransmission. Therefore, the D-serine/glycine-binding site in NMDAR is a major target for psychopharmacological interventions targeting plasticity-related disorders.
Developmental temperature alters the thermal sensitivity of courtship activity and signal-preference relationships, but not mating rates
Authors: Macchiano Anthony , Miller Em , Agali Uchechukwu , Ola-Ajose Abisiola , Fowler-Finn Kasey D.
Published: October 2022

Mating behaviors are sensitive to novel or stressful thermal conditions, particularly for ectothermic organisms. One way to deal with changes in thermal conditions is developmental plasticity, whereby the thermal sensitivity of mating behaviors depend on developmental conditions. We tested how developmental temperature affects the thermal sensitivity of courtship behavior and mating rates, as well as mating signal-preference relationships. To do so, we rear treehoppers under two temperature regimes and then test the expression of mating behaviors across a range of ambient temperatures for males and females. We find that developmental temperatures affect the thermal sensitivity of courtship behavior and mating signals for males, but not females. The sex-specific plasticity did not alter the thermal sensitivity of mating rates, but did disrupt signal-preference coupling across certain ranges of ambient temperature. We discuss the implications of signal-preference decoupling for sexual selection, how reversible acclimation may drive sex-specific results, and the potential for mismatches between developmental and mating thermal environments under future climate change predictions.
A Systematic Review of Interventions to promote HPV Vaccination Globally
Authors: Escoffery Cam , Petagna Courtney , Agnone Christine , Perez Stephen , Saber Lindsey , Ryan Grace , Dhir Meena , Sekar Swathi , Yeager Katherine A. , Biddell Caitlin B. , Madhivanan Purnima , English Amanda S. , Savas Lara , Daly Eliza , Vu Thuy , Lee Stephanie , Fernandez Maria E.
Published: October 2022

Background
Despite the human papillomavirus (HPV) vaccine being a safe, effective cancer prevention method, its uptake is subomptimal in the United States (U.S.). Previous research have found a variety of intervention strategies (environmental and behavioral) in increasing its uptake. The purpose of the study is to systematically review the literature for interventions to promote HPV vaccination from 2015 to 2020.
Methods
We updated a systemative review of interventions to promote HPV vaccine uptake globally. We ran key word searches in six bibliographic databases. Audiences, design, level of intervention, components and outcomes were abstracted from the full-text articles in Excel databases.
Results
Of the 79 articles, most were conducted in the U.S. (72.2%) and in clinical (40.5%) or school settings (32.9%), and were directed a single level (76.3%). Related to the intervention type, most were informational (n = 25, 31.6%) or patient-targeted decision support (n = 23, 29.1%). About 24% were multi-level interventions, with 16 (88.9%) combining two levels. Twenty-seven (33.8%) reported the use of theory in intervention development. Of those reporting HPV vaccine outcomes, post-intervention vaccine initiation ranged from 5–99.2%, while series completion ranged from 6.8–93.0%. Facilitators to implementation were use of patient navigators and user-friendly resources, while barriers included costs, time to implement and difficulties of integrating interventions into organizational workflow.
Conclusions
There is a strong need to expand implementation of HPV-vaccine promotion interventions beyond education alone and at a single level of intervention. Development and evaluation of effective strategies and multi-level interventions may increase the uptake of the HPV vaccine among adolescents and young adults.
Synthesis of grape-seed derived carbon with high specific surface area for CO2 selective adsorption
Authors: Luo Lan , Dang Wei , Zhang Li , Zhang Dan , Pan Hongyan , Lin Qian
Published: October 2022

Nitrogen-doped porous carbons with BET surface area of 1068.2-3314.5 m2/g and nitrogen contents of 3.2–6.5% were prepared from solid waste grape-seed via NaNH2 as activator and nitrogen source at lower activation temperature. The activation mechanism of NaNH2 on hydrothermal carbon precursors was first explored by thermodynamic analysis and TG-IR, which provided theoretical support for pore-forming of carbon materials. Maximum CO2 and CH4 adsorption capacity at 273 K and pressure of 101 KPa was 5.42 and 1.76 mmol/g respectively, which is higher than that of majority of solid waste derived carbons reported in literature. IAST selectivities of GS-3-450 with the largest BET surface area for CO2/CH4 (40v/60v), CO2/N2 (15v/85v), CH4/N2 (50v/50v) were found to be 20.3, 71.4, 6.0 under 101 kPa and 298K respectively. The competitive adsorption of GS-3-450 for CO2/CH4 (40v/60v), CO2/N2 (15v/85v), CH4/N2 (50v/50v) gases mixture were examined through breakthrough experiments, and the results showed the CO2 breakthrough time was longer than that of CH4 and N2, which was beneficial to the separation of CO2 from gases mixture. CH4 adsorption-desorption studies for 8 cycles revealed that the material exhibited excellent recycling stability. The lower temperature preparation method, excellent BET surface area and total pore volume and excellent CO2 and CH4 adsorption ability make it having a very good potential for the capture of CO2 and CH4.
Predicting Bicyclist Maneuvers using Explicit and Implicit Communication
Authors: Grigoropoulos Georgios , Malcolm Patrick , Keler Andreas , Busch Fritz , Bogenberger Klaus
Published: October 2022

Explicit and implicit communication cues are part of the communication strategies often employed by bicyclists in traffic to reveal their intentions to other road users. For human drivers, such types of information may be understandable in a specific social and cultural context. Present automated driving functions mostly rely on vulnerable road user (VRU) dynamic and operational features to perform short-term predictions of nearby VRU behaviour. The understanding of bicyclist implicit and explicit communication behavior can provide an extra layer of information in predicting their movement in traffic, confine the solution space to specific maneuver archetypes and expand the short-term time window for the bicyclist trajectory prediction. This paper focuses on the problem of predicting the bicyclist maneuver at an intersection approach using exclusively bicyclist explicit and implicit communication cues. Data is first evaluated using random forests for feature selection to determine the most important features for the prediction task. The next step involves training and testing different classifiers such as k-nearest neighbors, extra trees, decision trees, random forests, logistic regression, linear support vector machines, and the gaussian naive-bayes classifier to define the most efficient methodology to address the maneuver classification problem. Results suggest that the gaussian naive-bayes classifier provides the best performance on the test data with a 69.4% accuracy score during training and 67.0% accuracy score during testing. The proposed method has the potential to support and cooperate with existing solutions for bicyclist trajectory prediction as part of automated driving functions, effectively increasing traffic safety.
LINC02544 modulates the RAB/RAS signaling pathway by potential regulation of DENND2A expression level in the breast cancer patients: integrated bioinformatics and experimental analyses
Authors: Nezamnia Maria , Shirdeli Sayedeh Zahra , Sobhani Fatemeh , Moein Mina , Rahimirad Samira , Azadeh Mansoureh , Azhdari Sara
Published: October 2022

Background: One of the most common female malignancies is breast cancer (BC) and is recognized as a second death factor for women population among other cancer-related diseases. Dysregulation of RNA expression levels can lead human status to some different pathological statuses, including breast cancer. In this study, we have investigated the expression level of DENND2A and lncRNA LINC02544 in the BC tissue samples among Iranian population. Also, the clinicopathological and biomarker analysis of these RNAs were investigated.
Method: Microarray analysis was performed on GSE61304 in order to find the differentially expressed genes in the BC samples compared to controls. Multiple online and offline software, including R programming language and several relative statistical and visualization packages, demonstrated the differential expression level of genes,relative lncRNAs, and single nucleotide polymorphisms. The Real-time PCR results have demonstrated that the expression level of DENND2A has been reduced in the breast cancer samples compared with controls. In order to investigate the genotype frequency of rs6852 region in the DENND2A gene, high-resolution melt (HRM) method has been used.
Results of microarray and Real-time PCR analysis have been shown that the expression of DENND2A and lncRNA LINC02544 have significantly down-regulated in the breast cancer samples. These data suggested that these two RNAs are likely to respond to alter the expression level of each other.
Therefore, DENND2A could be an important prognostic biomarker in breast cancer samples among Iranian population. lncRNA LINC02544 could have an activator effect on the DENND2A expression. There is strong evidence that both of these RNAs could perform as tumor suppressors in breast cancer.
Mutagenesis and structural studies reveal the basis for the specific binding of SARS-CoV-2 SL3 RNA element with human TIA1 protein
Authors: Zhang Dong , Qiao Lulu , Wang Zhiye , Zhou Ruhong
Published: October 2022

Viral RNA-host protein interactions are indispensable during RNA virus transcription and replication. However, the detailed structural and dynamical features of the interactions between viral RNA and various host proteins remain largely elusive. Here, we characterized the binding interface for the SARS-CoV-2 stem-loop 3 (SL3) cis-acting element to human TIA1 protein with a combined theoretical and experimental approach including molecular modeling, free energy calculations, and electrophoretic mobility shift assays (EMSA). As a highly structured and conserved cis-acting element, SARS-CoV-2 SL3 RNA element was found to have a high binding affinity (Kd ~ 780 nM) to human TIA1 protein, with its hairpin and 3’-terminal loops playing essential roles in a sequence-dependent manner. Our molecular dynamics simulations revealed that the aromatic stacking, specific hydrogen bonds, and hydrophobic interactions collectively direct the specific binding of SL3 RNA element to TIA1, in which notable conformation changes both in protein domain arrangement and RNA 3D structure adaptation were observed. Further evaluations of in silico mutagenesis predictions with electrophoretic assays validated our proposed 3D binding model and also revealed SL3 A68U variant has an enhanced binding affinity (~ 1.7-fold) to TIA1 protein than the wild type. Finally, we found that the human TIA1 protein could interact with conserved SL3 RNA elements within other betacoronavirus lineages as well. These findings open a new avenue to explore the viral RNA-host protein interactions for SARS-CoV-2 infection and provide a pioneering structural basis for novel RNA-targeting antiviral drug design.
Leveraging Machine Learning Approaches for Predicting Potential Lyme Disease Cases and Incidence Rates in United States Using Twitter
Authors: Boligarla Srikanth , Laison Elda Kokoè Elolo , Li Jiaxin Fiona , Mahadevan Raja , Ng Austen , Lin Yangming , Thioub Mamadou Yamar , Huang Bruce , Ibrahim Mohamed Hamza , Nasri Bouchra
Published: October 2022

Background: Lyme disease is one of the most commonly reported infectious diseases in the United States (US), accounting for more than 90% of all vector-borne diseases in North America.
Objective: In this paper, self-reported tweets on Twitter were analyzed in order to predict potential Lyme disease cases and accurately assess incidence rates in the US.
Methods: The study was done in three stages: (1) Approximately 1.3 million tweets were collected and and pre-processed to extract the most relevant Lyme disease tweets with geolocations. The tweets were manually labelled as relevant or irrelevant to Lyme disease using a set of precise keywords, yielding a curated labelled dataset of 77500 tweets. (2) This labelled data set was used to train, validate, and test various combinations of NLP word embedding methods and prominent ML classification models, such as TF-IDF and logistic regression, Word2vec and XGboost, and BERTweet, among others, to identify potential Lyme disease tweets. (3) Lastly, the presence of spatio-temporal patterns in the US over a 10-year period were studied.
Results: Preliminary results showed that BERTweet outperformed all tested NLP classifiers for identifying Lyme disease tweets, achieving the highest classification accuracy and F1-score of 90%. There was also a consistent pattern indicating that the West and Northeast regions of the US had a higher tweet rate over time.
Conclusions: We focused on the less-studied problem of using Twitter data as a surveillance tool for Lyme disease in the US. Several crucial findings have emerged from the study. First, there is a fairly strong correlation between classified tweet counts and Lyme disease counts, with both following similar trends. Second, in 2015 and early 2016, the social media network like Twitter was essential in raising popular awareness of Lyme disease. Third, counties with a high incidence rate were not necessarily related with a high tweet rate, and vice versa. Fourth, BERTweet can be used as a reliable NLP classifier for detecting relevant Lyme disease tweets.
Parents' experiencees of life after medicalised conception: A systematic review and thematic meta-synthesis
Authors: Foyston Zoe , Higgins Lucy , Smith Debbie , Wittkowski Anja
Published: October 2022

Background: Medicalised Conception (MAC) assists many couples to achieve pregnancy worldwide. As the impact of MAC has been linked to increased pregnancy-specific anxiety and parenting difficulties, this review aimed to explore parental experiences of pregnancy and early parenting following MAC, identifying their psychological, social and health needs.
Method: Five databases were searched systematically. Identified articles were screened for eligibility. Twenty qualitative studies met inclusion criteria and results were analysed using thematic synthesis. The Critical Appraisal Skills checklist was employed to appraise methodological quality.
Results: The findings were synthesised into three main themes: 1) The vulnerable parent: fear, doubt, uncertainty, 2) the stark realisation of the parental dream, 3) psychosocial needs and support. Parents lacked a sense of safety during pregnancy and acted protectively, both antenatally and postnatally. Identity transition was complex and non-linear, influenced by sociocultural context.
Conclusions: Considerable unmet psychosocial needs were identified with a need for consistent, holistic care, integrating psychological services.
Early detection of chronic kidney disease in a diabetic population. A massive screening program in primary healthcare in a middle-income country
Authors: Miguel Mejía-Arias , Lastiri-Quirós H. Santiago , Obrador Gregorio T. , Palacio-Mejía Lina Sofía , Hernández-Avila Juan Eugenio , Amezcua Mario Márquez , Tamayo-Ortiz Marcela , Alvarez-Aceves Mariana , Guzmán-Sandoval Leslie , Hernández-Avila Mauricio , Tamayo-Orozco Juan Alfredo
Published: October 2022

Background
Early detection of chronic kidney disease (CKD) allows health professionals and patients to take actions to slow physical impairment and prevent or delay dialysis or transplantation and their catastrophic costs. In this study, we aimed to describe a CKD massive screening program in patients with diabetes to explore the feasibility of massive detection of CKD in high-risk patients.
Methods
We performed a descriptive, cross-sectional study with people living with diabetes, at the primary healthcare units of the Ministry of Health in Jalisco, Mexico. They underwent a screening including a health history questionnaire, somatometry, urinary albumin, serum creatinine, and estimated glomerular filtration rate (eGFR) in accordance with the Kidney Early Evaluation Program (KEEP). We described demographic and clinical characteristics of the population by sex and calculated the prevalence of each CKD stage.
Results
Of 7,693 recruited patients, 44% were identified with CKD; 35% were in early stages (1 or 2), and 9% in stages 3 to 5. Less than 1% of total patients had previous CKD diagnosis. Among other clinical characteristics, we found that 83% of all patients were overweight or obese and 79% had blood pressure values over 130/80 mmHg.
Conclusions
This study shows the technical feasibility and usefulness of massive CKD screening campaigns in high-risk people at primary healthcare services. Patients with renal function impairment, who require treatment for preventing disease progression, were identified. It is essential to formulate public policies aimed at protocolization of timely medical interventions to address the incidence and prevalence of CKD.
Numerical Simulations of Dynamic Replacement Method in Clay Lateritic Soils
Authors: de Souza Tennison Freire , Heineck Karla Salvagni , Ruver Cesar Alberto , Mohseni Ana Paula Vianna , Lopes Luizmar da Silva
Published: October 2022

The dynamic replacement (DR) method is a widespread alternative in several countries around the world as a technique to improve soils with low bearing capacity, compressible, collapsible, etc. It is an established method used on soft clays, silts, sandy silt, and/or sedimentary sandy soils with low bearing capacity. However, there are no published scientific reports on the use of this method on saprolite and/or laterite soils typical of tropical regions. Therefore, this paper aims to validate the use of the Mohr-Coulomb failure criterion using the finite element method (FEM) as a tool for predicting the elastoplastic behavior of lateritic clayey soils improved by the dynamic replacement method. For this purpose, 6 plate loading tests were performed on DR columns with and without cement-improved soil reinforcement layers (fine sand + Portland cement) with different heights and different DR column depths were numerically simulated in FEM based on the Mohr-Coulomb criterion. It was concluded that the use of the Mohr-Coulomb plastification criterion in FEM generated numerical simulations compatible with the responses of plate load tests performed in the field. In general, it was observed that the numerical model was able to represent the load vs. settlement behavior for different scenarios of varying granular column depth and reinforcement layer height.
Development of breeder chip for gene detection and molecular-assisted selection by target sequencing in wheat
Authors: Xiang Mingjie , Liu Shengjie , Wang Xiaoting , Zhang Mingming , Yan Weiyi , Wu Jianhui , Wang Qilin , Li Chunlian , Zheng Weijun , He Yilin , Ge Yunxia , Wang Changfa , Kang Zhensheng , Han Dejun , zeng qingdong
Published: October 2022

Wheat is an important food crop, but adversity stresses are becoming more common, resulting in significant yield losses. Accelerating molecularly assisted resistance breeding is critical. Through statistical analysis of published loci in wheat over the last two decades, we selected 60 loci with main breeding objectives, high heritability, and reliable genotyping, such as stress resistance, yield, plant height, and resistance to spike germination. Then, using genotyping by target sequencing(GBTS) technology, we developed a liquid phase chip based on 101 functional or closely linked markers. The genotyping of 42 loci was confirmed in an extensive collection of Chinese wheat cultivars, indicating that the chip can be used in molecular-assisted selection (MAS) for target breeding goals. Besides, we can perform the preliminary parentage analysis with the genotype data. The most significant contribution of this paper is to evaluate the target traits of breeding materials without conducting field experiments. Breeders can quickly screen germplasm resources, parental breeding materials, and intermediate materials for the presence of excellent allelic variants using the genotyping data by this chip, which is high throughput, convenient, reliable, and cost-efficient.
PGK1 is involved in the HIF-1 signaling pathway as a hub gene for ferroptosis after traumatic brain injury
Authors: Wang Zhao , Tian Jinjie , Wang Lei , Yan Hongyan , Feng Sujuan , Zhang Yi
Published: October 2022

The pathogenesis of ferroptosis in traumatic brain injury (TBI) is unclear, in this study, we aimed to identify key molecules associated with ferroptosis in TBI using bioinformatics analysis to determine its underlying mechanisms. GSE128543 dataset was downloaded from the Gene Expression Omnibus (GEO) database, and TBI-associated modules were obtained by weighted gene co-expression network analysis (WGCNA). We identified 60 differentially expressed genes (DEGs) by intersecting the modules with ferroptosis and glycolysis/gluconeogenesis gene libraries. The hypoxia-inducible factor-1 (HIF-1) signaling pathway was identified to be critical for ferroptosis post TBI, and protein–protein interaction (PPI) network identified 20 hub genes, including phosphoglycemicee kinase 1 (PGK1), ribosomal protein (RP) family, pyruvate kinase M1/2 (PKM), hypoxia-inducible factor 1α subunit (HIF-1α), and MYC genes. In this study, we further explored the role of PGK1, a gene involved in HIF-1 signaling pathway. Moreover, we constructed a TBI mice model and examined PGK1 and HIF-1α expression levels, and the results revealed their expressions increased after cortical injury in mice and they co-localized in the same cells. Furthermore, we examined the expressions of PGK1 in the cerebrospinal fluid of 20 clinical patients with different degrees of brain injuries within 48 h of surgery. The results revealed that PGK1 expression level was negatively correlated with the severity of the brain injury. These findings suggest that PGK1 may become a potential hub gene for ferroptosis via the HIF-1 signaling pathway, second to neurological injury after TBI, thereby affecting patient prognosis.
Deficiencies in the medical care of SMA: evidence from a nationwide patient registry in Germany
Authors: Leibrock Berenike , Landfeldt Erik , Hussong Justine , Huelle Tabea , Mattheus Hannah , Thiele Simone , Walter Maggie C , Zemlin Michael , Moehler Eva , Dillman Ullrich , Abner Sophia , Bastardas Marina Flotats
Published: October 2022

Background
Management and treatment of spinal muscular atrophy (SMA) has changed in recent years due to the introduction of novel transformative and potentially curative therapies resulting in the emergence of new disease phenotypes. Yet, little is known about the uptake and impact of these therapies in real-world clinical practice. The objective of this study was to describe current motor function, need of assistive devices, and therapeutic and supportive interventions provided by the healthcare system, as well as the socioeconomic situation of children and adults with different SMA phenotypes in Germany. We conducted a cross-sectional, observational study of German patients with genetically confirmed SMA identified and recruited via a nationwide SMA patient registry (www.sma-register.de) within the TREAT-NMD network. Study data was recorded directly from patient-caregiver pairs through a study questionnaire administered online via a dedicated study website.
Results
The final study cohort consisted of 107 patients with SMA. Of these, 24 were children and 83 adults. In total, about 78% of all participants were taking medication for SMA (predominantly nusinersen and risdiplam). All children with SMA1 were able to sit and 27% of children with SMA2 were able to stand or walk. Impaired upper limb function, scoliosis and bulbar dysfunction were observed more frequently in patients with reduced lower limb performance. Physiotherapy, occupational therapy, and speech therapy, as well as the use of cough assists were less common than indicated by care guidelines. Family planning and educational and employment status appear to be related to motor skill impairment.
Conclusions
We show that the natural history of disease has changed in Germany following improvements in SMA care and the introduction of novel therapies. Yet, a significant proportion of patients remain untreated. We also identified considerable limitations in rehabilitation and respiratory care, as well as low labour-market participation among adults with SMA, calling for action to improve the current situation.
Sexual violence is associated with poor treatment outcomes among transgender women living with HIV in Brazil
Authors: Leddy Anna M. , Veras Maria Amelia de Sousa Mascena , Saggese Gustavo Santa Roza , Bassichetto Katia Cristina , Gilmore Hailey J , Carvalho Paula Galdino Cardin , Maschião Luca Fasciolo , Neilands Torsten B. , Sevelius Jae , Lippman Sheri A.
Published: October 2022

Background: Transgender (trans) women are disproportionately affected by both HIV and gender-based violence (GBV), defined as physical, sexual, or emotional violence perpetrated against an individual based on their gender identity/expression. While a growing body of evidence demonstrates that GBV leads to poor HIV care and treatment outcomes among cisgender women, less research has examined this association among trans women. We assessed the impact of lifetime experiences of GBV on subsequent retention in HIV care and laboratory confirmed viral suppression among a sample of trans women living with HIV (TWH) in Brazil.
Methods: A pilot trial of a peer navigation intervention to improve HIV care and treatment among TWH was conducted in São Paulo, Brazil between 2018 and 2019. TWH were recruited and randomized into the intervention or control arm and participated in a baseline and 9-month follow-up survey and ongoing extraction of clinical visit, prescribing, and laboratory data. Generalized linear model regressions with a Poisson distribution estimated the relative risk (RR) for the association of lifetime physical and sexual violence reported at baseline with treatment outcomes at follow-up, adjusting for baseline sociodemographic characteristics.
Results: A total of 113 TWH participated in the study. At baseline, the median age was 30 years, and the prevalence of lifetime physical and sexual violence was 62% and 45%, respectively. At follow-up, 58% were retained in care and 35% had evidence of viral suppression. In adjusted models, lifetime physical violence was non-significantly associated with a 12% reduction in retention in care (RR: 0.88, 95% CI: 0.65, 1.19) and a 34% reduction in viral suppression (RR: 0.66; 95% CI: 0.40, 1.09). Lifetime sexual violence was significantly associated with a 28% reduction in retention in HIV care (RR: 0.72, 95% CI: 0.52, 0.99) and a 57% reduction in viral suppression (RR: 0.43; 95% CI: 0.23, 0.79).
Conclusion: Our findings are among the first to demonstrate that lifetime experiences of sexual violence are associated with subsequent reduced likelihood of retention in HIV care and viral suppression among trans women. Interventions seeking to improve HIV treatment outcomes should assess and address experiences of GBV among this population.
Trial registration: ClinicalTrials.gov Identifier: NCT03525340
The Feasibility of a Pea Protein Supplement with High Iron Bioavailability for Female Runners: A Randomized Control Trial
Authors: Shaw Keely A , Zello Gordon A , Lindsay Donna L , Warkentin Thomas D , Ko Jongbum , Chilibeck Philip D
Published: October 2022

Background: Iron deficiency is a global dilemma, with a high prevalence among females from both low- and high-income countries. Athletic females, especially those who engage in running, are at a particularly high risk of developing iron deficiency due to blood losses through menstruation coupled with decreased iron absorption secondary to exercise. Field peas, along with other plant-based protein sources, are rich in iron and inexpensive, making them an attractive option for those wishing to increase their iron intake without consuming more animal products. However, field peas are high in phytic acid, an inherent compound that binds to cations such as iron, forming a salt (phytate), and limiting absorption in the small intestine. The goal of the current research was to conduct a feasibility study to assess the acceptability of a high protein powder made from low phytic acid field peas in female runners.
Methods: Eight recreational female runners (age: 32.7 ±13.5 years; VO2max: 46.9±5.4 mL/kg/min) were assessed for iron status, body composition, and exercise performance before and after an 8-week intervention period, during which they supplemented with either a power derived from regular peas, a powder derived from peas with high iron bioavailability (low phytic acid), or maltodextrin.
Results: A high degree of compliance was observed, suggesting that supplementing with a high-protein pea powder derived from low phytic acid peas is feasible.
Conclusion: Techniques and protocol were acceptable and the values obtained were representative; therefore, a larger trial is warranted to investigate the role these peas might have in combating iron deficiency in athletes.
Trial Registration: NCT04872140