bluuebunny/crossref_metadata_2025 huggingface dataset:
Field Inoculation of Arbuscular Mycorrhizal Fungi Improves Fruit Quality and Root Physiological Activity of Citrus
Authors: Cao Ming-Ao , Wang Peng , Hashem Abeer , Wirth Stephan , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng
Published: December 2021

Soil arbuscular mycorrhizal (AM) fungi form a mutualistic symbiosis with plant roots and produce many benefits on host plants under potted conditions, while field inoculation of AM fungi on citrus (a woody plant) has been rarely reported. The present study aimed to analyze the changes in mycorrhizal growth, root vitality, and fruit quality of Citrus reticulata Blanco var. Ponkan mandarin cv. Jinshuigan grafted on Poncirus trifoliata L. after inoculation with a mix of AM fungi (Diversispora versiformis, Funneliformis mosseae, and Rhizophagus intraradices) and single F. mosseae. After the second year of AM fungal inoculations, root mycorrhizal colonization (%), root vitality, hyphal length in soil, and easily extractable glomalin-related soil protein content were significantly increased, while difficult-to-extract glomalin-related soil protein content was decreased. Two mycorrhizal fungal inoculation treatments collectively improved fruit quality parameters such as polar diameter, equatorial diameter, the weight of single fruits, fruit peel, and sarcocarp, coloration value, and soluble solids content. Our study, therefore, suggested that field inoculation with AM fungi improved root physiological activities in terms of mycorrhizal growth and root vitality and thus improved fruit quality. The effect of mixed-AM treatment was more significant than that of F. mosseae alone.
Effects of the Autumn Incorporation of Rice Straw and Application of Lime Nitrogen on Methane and Nitrous Oxide Emissions and Rice Growth of a High-Yielding Paddy Field in a Cool-Temperate Region in Japan
Authors: Takakai Fumiaki , Goto Mimori , Watanabe Haruki , Hatakeyama Keiko , Yasuda Kentaro , Sato Takashi , Kaneta Yoshihiro
Published: December 2021

The effects of autumn plowing and lime nitrogen application on rice straw decomposition, CH4 and N2O emission and rice growth in the following year in a high-yielding rice cultivated paddy field were evaluated for two years. The experimental plots were set up, combining different times of rice straw (750 g m−2) incorporation into the soil by plowing (autumn or the following spring), with and without lime nitrogen application in autumn (5 g-N m−2). Autumn plowing promoted the decomposition of rice straw, but the application of lime nitrogen did not show a consistent trend. The soil pH was high (7.3) at the studied site, and the alkaline effect of lime nitrogen may not have been significant. As with straw decomposition, CH4 emissions were suppressed by autumn plowing, and no effect from the lime nitrogen application was observed. It was also suggested that the straw decomposition period may be shorter and the CH4 emissions may be higher in high-yielding cultivars that require a longer ripening period than in normal cultivars. The effect of both treatments on N2O emission was not clear. Both the autumn plowing of rice straw and lime nitrogen application were effective in promoting rice growth and increasing rice yield.
Biostimulant Effects of an Aqueous Extract of Duckweed (Lemna minor L.) on Physiological and Biochemical Traits in the Olive Tree
Authors: Regni Luca , Del Buono Daniele , Miras-Moreno Begoña , Senizza Biancamaria , Lucini Luigi , Trevisan Marco , Morelli Venturi Diletta , Costantino Ferdinando , Proietti Primo
Published: December 2021

Biostimulants are becoming increasingly popular in agriculture for their ability to induce beneficial effects in crops, paving the way towards the identification of new materials with biostimulant potential. This study evaluated the potential of different concentrations of an aqueous extract (0.25%, 0.50%, and 1.00%, dry weight/water volume, respectively) obtained from duckweed (Lemna minor L.) to stimulate olive plants. Leaf net photosynthesis (Pn), leaf transpiration rate (E), stomatal conductance (gs), sub-stomatal CO2 concentration (Ci), chlorophyll content and other plant growth parameters were investigated. As a result, the extract improved Pn, gs, Ci, chlorophyll content and plant biomass production (leaf fresh and dry weight). Furthermore, the duckweed extract generally increased the uptake of nitrogen (N), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe) and zinc (Zn), while it did not influence the content of sodium (Na), manganese (Mn) and copper (Cu). The untargeted metabolomic profiling of the extract revealed the presence of signalling compounds (including phytohormones), phenolics and glutathione. Such broad diversity of bioactives may support the stimulatory potential observed in olive. In summary, this study revealed for the first time that duckweed could be seen as a promising species to obtain extracts with biostimulant properties in olive trees.
Robotic Mowing of Tall Fescue at 90 mm Cutting Height: Random Trajectories vs. Systematic Trajectories
Authors: Sportelli Mino , Fontanelli Marco , Pirchio Michel , Frasconi Christian , Raffaelli Michele , Caturegli Lisa , Magni Simone , Volterrani Marco , Peruzzi Andrea
Published: December 2021

Tall fescue (Schedonorus arundinaceus (Schreb.) Dumort.) is often managed with a cutting height ranging from 70 to 100 mm in ornamental lawns. Some autonomous mowers have been specifically designed to maintain mowing height in the same range. Generally, autonomous mowers operate by following random trajectories, and substantial overlapping is needed to obtain full coverage of the working area. In the case of tall grass, this may cause lodging of grass plants, which in turn may reduce turf quality. The introduction of a navigation system based on systematic trajectories has the potential to improve the performances of autonomous mowers with respect to machine efficiency and turf quality. With the aim of determining the effects of reduced mowing frequency and systematic navigation systems on turf quality and mower performances in terms of working time, energy consumption and overlapping, the performances of two autonomous mowers working with random and systematic trajectories were tested on a mature tall fescue lawn at 90 mm cutting height. The working efficiency was approximately 80% for the systematic trajectories and approximately 35% for the random trajectories; this was mainly due to the lower overlapping associated with systematic trajectories. Turf quality was slightly higher for the mower working systematically (a score of 8 using a 1–9 score with 1 = poor, 6 = acceptable and 9 = best) compared to the one working randomly (quality of 7 and 6 on a 1–9 scale with 1 = poor and 9 = best). No appreciable lodging was observed in either case. For tall, managed lawns, systematic trajectories may improve autonomous mowers’ overall performances.
The Effect of Microwave Treatment on Germination and Health of Carrot (Daucus carota L.) Seeds
Authors: Szopińska Dorota , Dorna Hanna
Published: December 2021

The aim of the study was to evaluate the effect of microwave treatment on seed germination and health of carrot seeds using two seed lots naturally infected with the pathogens Alternaria dauci and A. radicina. Seeds of cv. Amsterdam and cv. Berlikumer varied in seed germination at the final count (50% and 29%, respectively), and seed infestation with A. radicina (38% and 5%, respectively). For treatment, seeds were placed in a Petri dish (dry treatment) or in a beaker with distilled water (wet treatment) and irradiated at power output levels 500, 650 and 750 W for 15, 30, 45, 60, 75, and 90 s. Germination and health were determined in treated and untreated (control) seed samples. Wet treatment controlled seed-borne fungi more efficiently than dry treatment. However, the exposure duration longer than 60 s frequently resulted in deterioration of seed germination. The highest seed germination in cv. Amsterdam was observed after microwave wet treatment at power output levels of 500 W for 75 s (81%), 650 W for 45 s (85%), and 750 W for 60 s (77%), whereas in the case of cv. Berlikumer this occurred when wet seeds were treated at 500 and 650 W for 60 s (46% and 43% respectively). Treating seeds soaked in water with microwaves for a period longer than 30 s, regardless of the power output, significantly decreased seed infestation with Alternaria spp. in both samples.
Farm-Scale Crop Yield Prediction from Multi-Temporal Data Using Deep Hybrid Neural Networks
Authors: Engen Martin , Sandø Erik , Sjølander Benjamin Lucas Oscar , Arenberg Simon , Gupta Rashmi , Goodwin Morten
Published: December 2021

Farm-scale crop yield prediction is a natural development of sustainable agriculture, producing a rich amount of food without depleting and polluting environmental resources. Recent studies on crop yield production are limited to regional-scale predictions. The regional-scale crop yield predictions usually face challenges in capturing local yield variations based on farm management decisions and the condition of the field. For this research, we identified the need to create a large and reusable farm-scale crop yield production dataset, which could provide precise farm-scale ground-truth prediction targets. Therefore, we utilise multi-temporal data, such as Sentinel-2 satellite images, weather data, farm data, grain delivery data, and cadastre-specific data. We introduce a deep hybrid neural network model to train this multi-temporal data. This model combines the features of convolutional layers and recurrent neural networks to predict farm-scale crop yield production across Norway. The proposed model could efficiently make the target predictions with the mean absolute error of 76 kg per 1000 m2. In conclusion, the reusable farm-scale multi-temporal crop yield dataset and the proposed novel model could meet the actual requirements for the prediction targets in this paper, providing further valuable insights for the research community.
Multicolor Fluorescence Imaging for the Early Detection of Salt Stress in Arabidopsis
Authors: Tian Ya , Xie Limin , Wu Mingyang , Yang Biyun , Ishimwe Captoline , Ye Dapeng , Weng Haiyong
Published: December 2021

Salt stress is one of the abiotic factors that causes adverse effects in plants and there is an urgent need to detect salt stress in plants as early as possible. Multicolor fluorescence imaging, as a powerful tool in plant phenotyping, can provide information about primary and secondary metabolism in plants to detect the responses of the plants exposed to stress in the early stage. The purpose of this study was to evaluate the potential of multicolor fluorescence imaging’s application in the early detection of salt stress in plants. In this study, the measurements were conducted on Arabidopsis and the multicolor fluorescence images were acquired at 440, 520, 690, and 740 nm with a self-developed imaging system consisting of a UV light-emitting diode (LED) panel for an excitation at 365 nm, a charge coupled device (CCD) camera, interference filters, and a computer. We developed a classification method using the imaging analysis of multicolor fluorescence based on principal component analysis (PCA) and a support vector machine (SVM). The results showed that the four principal fluorescence feature combinations were the ideal indicators as the inputs of the SVM model, and the classification accuracies of the control and salt-stress treatment at 5 days and 9 days were 92.65% and 98.53%, respectively. The results indicated that multicolor fluorescence imaging combined with PCA and SVM could act as a tool for early detection in salt-stressed plants.
Mapping Gaps in Sugarcane by UAV RGB Imagery: The Lower and Earlier the Flight, the More Accurate
Authors: Barbosa Júnior Marcelo Rodrigues , Tedesco Danilo , Corrêa Rafael de Graaf , Moreira Bruno Rafael de Almeida , Silva Rouverson Pereira da , Zerbato Cristiano
Published: December 2021

Imagery data prove useful for mapping gaps in sugarcane. However, if the quality of data is poor or the moment of flying an aerial platform is not compatible to phenology, prediction becomes rather inaccurate. Therefore, we analyzed how the combination of pixel size (3.5, 6.0 and 8.2 cm) and height of plant (0.5, 0.9, 1.0, 1.2 and 1.7 m) could impact the mapping of gaps on unmanned aerial vehicle (UAV) RGB imagery. Both factors significantly influenced mapping. The larger the pixel or plant, the less accurate the prediction. Error was more likely to occur for regions on the field where actively growing vegetation overlapped at gaps of 0.5 m. Hence, even 3.5 cm pixel did not capture them. Overall, pixels of 3.5 cm and plants of 0.5 m outstripped other combinations, making it the most accurate (absolute error ~0.015 m) solution for remote mapping on the field. Our insights are timely and provide forward knowledge that is particularly relevant to progress in the field’s prominence of flying a UAV to map gaps. They will enable producers to make decisions on replanting and fertilizing site-specific high-resolution imagery data.
Selection of Elite dura-Type Parents to Produce Dwarf Progenies of Elaeis guineensis Using Genetic Parameters
Authors: Tupaz-Vera Andrés , Ayala-Diaz Iván , Barrera Carlos Felipe , Romero Hernán Mauricio
Published: December 2021

The low annual growth rate of the stipe in oil palm progenies is desirable to increase these crops’ productive and economic life. Recurrent reciprocal selection (R.R.S.) has allowed the development of oil palm populations through several breeding cycles with an increased frequency of favorable alleles associated with traits of interest. The present study evaluated families derived from Deli dura × African dura crosses. For 12 years, the yield, vegetative characteristics, and the amount of oil in seven dura progenies were assessed to estimate, from the information collected, the genetic parameters, heritability, and phenotypic correlations among quantitative genetic traits of high-yielding dwarf progenies. The analysis was carried out using analysis of variance, followed by a comparison of means for all estimated traits. The effect of the progenies was highly significant (p ≤ 0.01) for most traits. The yield values, expressed in fresh fruit bunches (FFB) for the progenies, ranged from 165 to 208 kg per palm per year. The oil-to-bunch ratio (O/B) ranged from 17% to 19%, with an overall average of 18%. One of the essential characteristics in this study was the vertical growth of the stipe. Progenies P6 and P7 were identified as those with the lowest annual increase in height, with values of 0.29 and 0.33 m year−1. The values indicate that these are slow-growing cultivars with a high FFB yield and O/B. The highest heritabilities were found for the vegetative trait height (71.62%) and the number of leaflets (46.64%). The development of dura parents with slow growth characteristics in combination with a high bunch and oil production allows extending the productive life of the crop to more than 35 years, providing added value to obtaining differentiated cultivars of oil palm.
Effect of Soil-Applied L-tryptophan on the Amount of Biomass and Nitrogen and Sulfur Utilization by Maize
Authors: Gondek Krzysztof , Mierzwa-Hersztek Monika
Published: December 2021

As natural plant growth stimulators, amino acids are widely used to improve crop yield and quality. There are numerous studies documenting the influence of amino acids on plants, which is not always positive. This study was conducted to determine the effect of soil-applied L-tryptophan (L-TRP) on the accumulation and utilization of nitrogen and sulfur by maize. The study was carried out under the conditions of a pot experiment. The experimental design included three treatments: soil without fertilization (control), soil with mineral fertilization (NPKS), and soil with mineral fertilization and L-tryptophan addition (NPKS + L-TRP). The application of tryptophan to the soil, supported by mineral fertilization, caused a significant increase in maize biomass. Although no significant differences in nitrogen and sulfur contents in maize biomass were found between treatments without and with the addition of L-tryptophan, significantly higher intakes of both elements were observed in the NPKS + L-TRP treatment. The application of L-tryptophan increased the biosynthesis of Chlorophyll a. Utilization of nitrogen and sulfur by maize in the NPKS + L-TRP treatment was more than 27% and 17% higher, respectively, compared to the NPKS treatment. Maintaining the recommended contents of individual nutrients in the rhizosphere is not a guarantee of optimal quantitative and qualitative intake of nutrients. Problems with maintaining optimal relationships between individual nutrients may be compounded by soil properties.
Evaluation of Crossability between Nicotiana benthamiana and Nicotiana excelsior
Authors: Tezuka Takahiro , Kitamura Naoto , Yanase Masanori , Morikawa Toshinobu
Published: December 2021

Wild tobacco species in the Nicotiana section Suaveolentes are promising genetic resources to introduce their disease resistance to cultivated tobacco, Nicotiana tabacum. However, hybrid lethality is observed in hybrid seedlings from crosses between most Suaveolentes species and N. tabacum. In particular, N. benthamiana belonging to the section Suaveolentes produces only viable hybrids after crossing with N. tabacum. In the present study, crossability between N. benthamiana and N. excelsior (section Suaveolentes) was investigated to test the possible usefulness of N. benthamiana as the bridge parent to transfer desirable genes of N. excelsior to N. tabacum via bridge crossing. After reciprocal crosses using three accessions of N. benthamiana and N. excelsior each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between N. benthamiana and N. excelsior showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between N. benthamiana and N. excelsior is discussed.
Improving Nitrogen Status Estimation in Malting Barley Based on Hyperspectral Reflectance and Artificial Neural Networks
Authors: Klem Karel , Křen Jan , Šimor Ján , Kováč Daniel , Holub Petr , Míša Petr , Svobodová Ilona , Lukas Vojtěch , Lukeš Petr , Findurová Hana , Urban Otmar
Published: December 2021

Malting barley requires sensitive methods for N status estimation during the vegetation period, as inadequate N nutrition can significantly limit yield formation, while overfertilization often leads to an increase in grain protein content above the limit for malting barley and also to excessive lodging. We hypothesized that the use of N nutrition index and N uptake combined with red-edge or green reflectance would provide extended linearity and higher accuracy in estimating N status across different years, genotypes, and densities, and the accuracy of N status estimation will be further improved by using artificial neural network based on multiple spectral reflectance wavelengths. Multifactorial field experiments on interactive effects of N nutrition, sowing density, and genotype were conducted in 2011–2013 to develop methods for estimation of N status and to reduce dependency on changing environmental conditions, genotype, or barley management. N nutrition index (NNI) and total N uptake were used to correct the effect of biomass accumulation and N dilution during plant development. We employed an artificial neural network to integrate data from multiple reflectance wavelengths and thereby eliminate the effects of such interfering factors as genotype, sowing density, and year. NNI and N uptake significantly reduced the interannual variation in relationships to vegetation indices documented for N content. The vegetation indices showing the best performance across years were mainly based on red-edge and carotenoid absorption bands. The use of an artificial neural network also significantly improved the estimation of all N status indicators, including N content. The critical reflectance wavelengths for neural network training were in spectral bands 400–490, 530–570, and 710–720 nm. In summary, combining NNI or N uptake and neural network increased the accuracy of N status estimation to up 94%, compared to less than 60% for N concentration.
Influence of Geometry in the Behavior of Disc-Shaped Mechanical Energy Absorbers for Agricultural Tractors
Authors: Latorre-Biel Juan-Ignacio , Pérez-Ezcurdia Amaya , Benito-Amurrio Marta , Alfaro-López José Ramón
Published: December 2021

Disc-shaped mechanical energy absorbers (MEAs), in combination with rollover protection structures (ROPSs), may contribute to the prevention of the infringement of the safety zone and the collapse of the ROPS in case of the overturn of an agricultural tractor. An MEA can absorb a significant amount of potential energy of an overturning tractor and its deformation produces a rotation of the ROPS around the safety zone. In this research, MEAs with two different geometries have been developed. Both geometries present common features, such as disc dimensions, number of rings, and number of arms, but the distribution of the arms differs. Additionally, these MEA were manufactured in steel discs of four different thicknesses, ranging from 2 to 6 mm. The manufactured MEAs were tested in a universal testing machine, and their behavior characterized. From this data, linear models of the MEAs were developed. As a consequence, a number of characteristic parameters were selected and calculated, such as the activation load and the strain energy absorbed in a safe range of applied loads. Some patterns and trends were analyzed from the tested MEAs, which enables a better description of their behavior and the extrapolation of this behavior to other non-tested thicknesses and geometries.
The Ethics of Innovation: Ethical Decision-Making and Review for Field Studies and Projects Targeting Dogs and Cats
Authors: Benka Valerie A.
Published: December 2021

To date, independent ethical oversight of many companion animal welfare initiatives has been limited and, in some instances, inadequate. Beyond a blurred line between “innovation” and “research,” the nature of the work conducted in animal welfare projects is often poorly aligned with established institutional ethical review structures, which are designed for research involving humans or research involving animals and are also focused on industry and academic institutions. This commentary details the struggle of one United States-based nonprofit organization to find ethical guidelines and support for conducting non-traditional field-based animal welfare studies, and subsequent experience establishing an Ethical Review Board to evaluate organizational initiatives. The commentary discusses member selection, materials and processes, and lessons and learnings from the creation and use of an Ethical Review Board. Sharing content of the ethical review process, as well as challenges and learnings from it, is intended to support other organizations and individuals seeking to ensure that innovation for animal welfare consistently meets high ethical standards.
Very Small Home Ranges of Two Gravid European Brown Bears during Hyperphagia
Authors: Schulte Laura , De Angelis Daniele , Babic Natarsha , Reljić Slaven
Published: December 2021

In September 2019, two gravid female brown bears (Ursus arctos) were captured and equipped with GPS/GSM collars in Paklenica National Park (Croatia). Home ranges during hyperphagia were analyzed to describe the spatiotemporal requirements. Mean seasonal home ranges were very small with 9.2 km2 and 7.5 km2 (Brownian Bridge Movement Model 95%). During the tracking period, both bears used different territories and showed little to no use of overlapping area. The bears in our study spent a considerable time in proximity of artificial feeding sites, indicating a probable use of these structures as a food resource (mean 15.7% and 30.7%). Furthermore, the bears approached very close to human structures such as 8.9 m and 4.4 m. As most encounters between humans and bears occur during hyperphagia, it is important to offer refugia from human disturbance, especially as the National Park is not only used by residents, but also by tourists. To adapt management according to the animal’s needs, further studies should include more individuals from different age and sex classes. Both females were gravid. It remains unclear whether gravidity has an effect on the home range and should be further investigated.
Effects of Dietary Resveratrol Supplementation on Growth Performance and Anti-Inflammatory Ability in Ducks (Anas platyrhynchos) through the Nrf2/HO-1 and TLR4/NF-κB Signaling Pathways
Authors: Yang Hao , Wang Yingjie , Liu Mengru , Liu Xiao , Jiao Yihan , Jin Sanjun , Shan Anshan , Feng Xingjun
Published: December 2021

The aim of this study was to explore the effect of dietary resveratrol on the growth performance and anti-inflammatory mechanism in ducks. A total of 280 one-day-old specific pathogen-free male ducklings (Anas platyrhynchos) with an average body weight of 35 ± 1 g were randomly divided into two dietary treatment groups with different supplementation levels of resveratrol for growth performance experiments: R0 and R400 (0 and, 400 mg kg−1 resveratrol, respectively). At the age of 28 days, 16 ducks were selected from each treatment group and divided into four subgroups for a 2 × 2 factorial pathological experiment: R0; R400; R0 + LPS; R400 + LPS, (0 mg kg−1 resveratrol, 400 mg kg−1 resveratrol, 0 mg kg−1 resveratrol, 400 mg kg−1 resveratrol + 5 mg lipopolysaccharide/kg body weight). The results showed that resveratrol significantly improved final body weight and average daily gain (p < 0.01) and alleviated the lipopolysaccharide-induced inflammatory response with a reduction in IL-1β and IL-6 in the plasma and the liver (p < 0.05). Resveratrol improved mRNA levels of Nrf2 and HO-1 and decreased the mRNA levels of TLR4 and NF-κB in duck liver (p < 0.05). Dietary resveratrol can improve growth performance and reduce inflammation through the Nrf2/HO-1 and TLR4/NF-κB signaling pathways in duck.
Occurrence of Antimicrobial Resistance Genes in the Oral Cavity of Cats with Chronic Gingivostomatitis
Authors: Tsang Wayne , Linde Annika , Krumbeck Janina A. , Wu Guangxi , Kim Young J. , Lushington Gerald H. , Melgarejo Tonatiuh
Published: December 2021

Feline chronic gingivostomatitis (FCGS) is a severe immune-mediated inflammatory disease with concurrent oral dysbiosis (bacterial and fungal). Broad-spectrum antibiotics are used empirically in FCGS. Still, neither the occurrence of antimicrobial-resistant (AMR) bacteria nor potential patterns of co-occurrence between AMR genes and fungi have been documented in FCGS. This study explored the differential occurrence of AMR genes and the co-occurrence of AMR genes with oral fungal species. Briefly, 14 clinically healthy (CH) cats and 14 cats with FCGS were included. Using a sterile swab, oral tissue surfaces were sampled and submitted for 16S rRNA and ITS-2 next-generation DNA sequencing. Microbial DNA was analyzed using a proprietary curated database targeting AMR genes found in bacterial pathogens. The co-occurrence of AMR genes and fungi was tested using point biserial correlation. A total of 21 and 23 different AMR genes were detected in CH and FCGS cats, respectively. A comparison of AMR-gene frequencies between groups revealed statistically significant differences in the occurrence of genes conferring resistance to aminoglycosides (ant4Ib), beta-lactam (mecA), and macrolides (mphD and mphC). Two AMR genes (mecA and mphD) showed statistically significant co-occurrence with Malassezia restricta. In conclusion, resistance to clinically relevant antibiotics, such as beta-lactams and macrolides, is a significant cause for concern in the context of both feline and human medicine.
Effect of Streptococcus uberis on Gamma Delta T Cell Phenotype in Bovine Mammary Gland
Authors: Slama Petr , Zavadilova Terezie , Pavlik Ales , Horky Pavel , Skalickova Sylvie , Skladanka Jiri , Roychoudhury Shubhadeep , Baldovska Simona , Kolesarova Adriana , Konecny Roman , Tancin Vladimir , Zouharova Monika
Published: December 2021

In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.
Utero-Placental Immune Milieu during Normal and Aglepristone-Induced Parturition in the Dog
Authors: Tavares Pereira Miguel , Nowaczyk Renata , Aslan Selim , Ay Serhan S. , Kowalewski Mariusz P.
Published: December 2021

Maternal immunotolerance is required for the maintenance of pregnancy, in sharp contrast with the uterine pro-inflammatory activity observed during parturition in several species. Correspondingly, in the dog, increased immune signaling at term has been suggested, but a deeper understanding of the uterine immune milieu is still missing. Thus, the availability of 30 immune-related factors was assessed in utero-placental samples collected during post-implantation (days 18–25 of pregnancy) and mid-gestation (days 35–40) stages, and at the time of prepartum luteolysis. Gene expression and/or protein localization studies were employed. Samples collected from antigestagen (aglepristone)-treated dogs were further analyzed. Progression of pregnancy was associated with the downregulation of IL1β and upregulation of IL10 (p < 0.05) at mid-gestation. When compared with mid-gestation, a higher availability of several factors was observed at term (e.g., CD206, CD4, TLR4). However, in contrast with natural parturition, MHCII, CD25, CCR7, TNFα, IDO1 and AIF1 were upregulated after aglepristone treatment (p < 0.05), but not TNFR1 or CCL13 (p > 0.05). Altogether, these results show an increased immune activity during canine parturition, involving, i.a., M2 macrophages, Treg and Th cells, with strong support for progesterone-mediated immunomodulation. Furthermore, differences between term and induced parturition/abortion could relate to differences in placental maturation towards parturition and/or functional traits of antigestagens.
Effects of Dietary Supplementation with Mushroom or Vitamin D2-Enriched Mushroom Powders on Gastrointestinal Health Parameters in the Weaned Pig
Authors: Dowley Alison , Sweeney Torres , Conway Eadaoin , Vigors Stafford , Yadav Supriya , Wilson Jude , Gabrielli William , O’Doherty John V.
Published: December 2021

The objective of this study was to compare the molecular, physiological and microbial effects of mushroom powder (MP), vitamin D2 enriched mushroom powder (MPD2) and zinc oxide (ZnO) in pigs post-weaning. Pigs (four pigs/pen; 12 pens/treatment) were assigned to: (1) basal diet (control), (2) basal diet + ZnO, (3) basal diet + MP (2 g/kg feed) and (4) basal diet + MPD2 (2 g/kg feed). Zinc oxide supplementation improved the feed intake (p < 0.001); increased the caecal abundance of Lactobacillus (p < 0.05); increased the villus height (p < 0.05) in the duodenum, jejunum and ileum; increased the expression of chemokine interleukin 8 (CXCL8; p < 0.05); and decreased the expression of pro-inflammatory cytokine gene interleukin 6 (IL6; p < 0.05), tumour necrosis factor (TNF; p < 0.05), nutrient transporters peptide transporter 1 (SLC15A1; p < 0.05) and fatty acid binding protein 2 (FABP2; (p < 0.05) in the duodenum. Whereas dietary supplementation with MPD2 improved the gastrointestinal morphology (p < 0.05); increased the total volatile fatty acid concentrations (p < 0.05); increased the expression of anti-inflammatory cytokine gene interleukin 10 (IL10; p < 0.05) and nutrient transporters SLC15A1 (p < 0.05), FABP2 (p < 0.05) and vitamin D receptor (VDR; p < 0.05); and reduced the expression of pro-inflammatory cytokine gene IL6 (p < 0.05), it adversely affected average daily feed intake (ADFI; p < 0.001) and average daily gain (ADG; p < 0.05). Mushroom powder supplementation had a positive impact on gastrointestinal morphology (p < 0.05) and upregulated the expression of nutrient transporters SLC15A1 (p < 0.05) and FABP2 (p < 0.05) and tight junction claudin 1 (CLDN1) (p < 0.05) compared to the controls but had no effect on the expression of inflammatory markers (p > 0.05). Furthermore, MP reduced ADFI (p < 0.01); however, this did not negatively impact the ADG (p > 0.05). In conclusion, MP and MPD2 have limited use as commercial feed additives in replacing ZnO in pig diets as feed intake was reduced post-weaning.
Assessment of Lung Disease in Finishing Pigs at Slaughter: Pulmonary Lesions and Implications on Productivity Parameters
Authors: Paz-Sánchez Yania , Herráez Pedro , Quesada-Canales Óscar , Poveda Carlos G. , Díaz-Delgado Josué , Quintana-Montesdeoca María del Pino , Plamenova Stefanova Elena , Andrada Marisa
Published: December 2021

Swine respiratory disease is associated with productive losses. We evaluated the prevalence of lung lesions with an emphasis on Mycoplasma hyopneumoniae (Mh), porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as the impact on productive parameters in 108 finishing pigs at slaughter. Pathologic, immunohistochemical (IHC) and serologic analyses were performed. Pneumonic processes were observed in 73.1% of the animals. They mainly consisted of cranioventral bronchopneumonia (CBP) (46.3%) and pleuritis (17.6%). Microscopically, bronchointerstitial pneumonia (67.4%) was common and was occasionally combined (27.9%) with interstitial pneumonia (IP). Mh and PCV2-antigens were detected in bronchointerstitial pneumonia (70.7%) and IP cases (33.3%). There were low titers against Mh (18%) and high titers against PRRSV (100%) and PCV2 (65%). Animals with CBP remained at the farm longer; those with >10% of lung parenchyma involvement were sent later (208.8 days old) and had a lower average carcass weight (74.1 kg) and a lower daily weight gain (500.8 gr/day) compared with animals without lesions (567.2 gr/day, 77.7 kg, 200.8 days old). We suggest that animals that do not reach the weight at slaughter should be sent to slaughter regardless to avoid further negative impacts of respiratory disease in productive parameters.
The Efficiency of Probiotics Administrated via Different Routes and Doses in Enhancing Production Performance, Meat Quality, Gut Morphology, and Microbial Profile of Broiler Chickens
Authors: Soumeh Elham A. , Cedeno Astrid Del Rocio Coba , Niknafs Shahram , Bromfield Jacoba , Hoffman Louwrens C.
Published: December 2021

To study the efficiency of Bacillus spp. probiotics administered via different routes and doses, a 6-week grow-out trial was conducted using a total of 378 day-old mixed-sex ROSS308 broiler chickens in a completely randomized block design. Six experimental diets included probiotics added at two different inclusion rates into the feed (250 g/ton; PRO250, or 500 g/ton; PRO500), or in the drinking water (25 g/L; PRO-WS), or as a feed synbiotic (250 g probiotic + 250 g/ton prebiotic; SYN), compared to a negative (NC; without additives) and positive control (PC; with antibiotics) diets. The PRO-WS enhanced feed intake (p < 0.05) and tended to improve average daily gain and final body weight (p = 0.14). Broiler gut morphology in the duodenum including the villus height (p = 0.04), villus width (p = 0.05) and crypt depth (p = 0.02) were improved by PRO500. Firmicutes was the most abundant phylum, followed by Bacteroidetes. Streptococcaceae, Lachnoospiraceae, Peptostreptococcaceae, Ruminococcaceae, and Erysipe-lotrichaceae were the top five most abundant families. Antibiotic inclusion in PC reduced microbial beta-diversity and increased similarity compared to probiotic inclusion (p = 0.05). Probiotic inclusion reduced the relative abundance of Bacteroides fragilis, which is a commonly isolated pathogen and is considered as a marker for antimicrobial resistance. Overall, probiotic supplementation via feed or water may potentially improve the production performance of the broiler chickens, and water-soluble probiotics are potentially more effective. Probiotics, especially when added to water, suggest a promising feed additive to support gut microbial maturation and diversity, and may reduce resistant bacteria in broiler chickens. However, it is suggested that the best route for the administration of probiotics be further examined under commercial conditions to find the most effective and practical application method that yields the most consistent results.
Tryptophan, Kynurenine and Kynurenic Acid Concentrations in Milk and Serum of Dairy Cows with Prototheca Mastitis
Authors: Bochniarz Mariola , Piech Tomasz , Kocki Tomasz , Iskra Mateusz , Krukowski Henryk , Jagielski Tomasz
Published: December 2021

The aim of this work was to investigate serum and milk levels of tryptophan (TRP), kynurenine (KYN), and kynurenic acid (KYNA), as well as the activity of indoleamine 2,3-dioxygenase (IDO) in cows with mastitis due to Prototheca algae. The study was prompted by previous research showing a link between the KYN pathway of TRP metabolism and bovine mastitis of bacterial etiology. The study was carried out over a 2-year period (2018–2019) and included quarter milk and serum samples collected from six dairy herds in Poland. The samples were obtained from healthy cows and cows with Prototheca mastitis of either clinical and subclinical manifestation, as determined upon direct measurement of the somatic cell count or indirectly by performing a California Mastitis Test on suspected quarters. Both TRP and KYN concentrations were significantly lower in milk of mastitic cows compared to healthy animals (0.8 vs. 8.72 µM, p = 0.001; 0.07 vs. 0.32 µM, p = 0.001, respectively). The difference in TRP and KYN concentrations in the sera of the two animal groups was much less pronounced (25.55 vs. 27.57 µM, 3.03 vs. 3.56 nM, respectively). The concentration of KYNA was almost at the same level in milk (1.73 vs. 1.70 nM) and in serum (80.47 vs. 75.48 nM) of both mastitic and healthy cows. The data showed that the level of TRP and its metabolites in serum was conspicuously higher compared to milk in all cows under the study. The activity of IDO was significantly higher in milk of cows with Prototheca mastitis compared to healthy animals (71.4 vs. 40.86, p < 0.05), while in serum it was pretty much the same (135.94 vs. 124.98, p > 0.05). The IDO activity differed significantly between serum and milk both for mastitic (135.94 vs. 71.4, p < 0.05) and healthy cows (124.98 vs. 40.86, p < 0.001). In conclusion, low values of TRP and KYN concentrations or elevated IDO activity in milk samples might be used as markers of mastitis due to infectious causes, including Prototheca spp.
Prevalence and Antimicrobial Resistance of Enterococcus Species: A Retrospective Cohort Study in Italy
Authors: Boccella Mariarosaria , Santella Biagio , Pagliano Pasquale , De Filippis Anna , Casolaro Vincenzo , Galdiero Massimiliano , Borrelli Anna , Capunzo Mario , Boccia Giovanni , Franci Gianluigi
Published: December 2021

Antimicrobial resistance represents one of the main threats to healthy ecosystems. In recent years, among the multidrug-resistant microorganisms responsible for nosocomial infections, the Enterococcus species have received much attention. Indeed, Enterococcus have peculiar skills in their ability to acquire resistance genes and to cause severe diseases, such as endocarditis. This study showed the prevalence and antimicrobial resistance rate of Enterococcus spp. isolated from clinical samples, from January 2015 to December 2019 at the University Hospital “San Giovanni di Dio e Ruggi d’Aragona” in Salerno, Italy. A total of 3236 isolates of Enterococcus faecalis (82.2%) and Enterococcus faecium (17.8%) were collected from urine cultures, blood cultures, catheters, respiratory tract, and other samples. Bacterial identification and antibiotic susceptibility were performed with VITEK 2. E. faecium showed a high resistance rate against ampicillin (84.5%), ampicillin/sulbactam (82.7%), and imipenem (86.7%), while E. faecalis showed the highest resistance rate against gentamicin and streptomycin high level, but both were highly sensitive to such antibiotics as tigecycline and vancomycin. Studies of surveillance are an important tool to detect changes in the resistance profiles of the main pathogens. These antimicrobial susceptibility patterns are necessary to improve the empirical treatment guideline of infections.
Antibiotic and Heavy Metal Susceptibility of Non-Cholera Vibrio Isolated from Marine Sponges and Sea Urchins: Could They Pose a Potential Risk to Public Health?
Authors: Costa Wellington Felipe , Giambiagi-deMarval Marcia , Laport Marinella Silva
Published: December 2021

Vibrio is an important human and animal pathogen that can carry clinically relevant antibiotic resistance genes and is present in different aquatic environments. However, there is a knowledge gap between antibiotic and heavy metal resistance and virulence potential when it is part of the microbiota from marine invertebrates. Here, we aimed to evaluate these characteristics and the occurrence of mobile genetic elements. Of 25 non-cholera Vibrio spp. from marine sponges and sea urchins collected at the coastlines of Brazil and France analyzed in this study, 16 (64%) were non-susceptible to antibiotics, and two (8%) were multidrug-resistant. Beta-lactam resistance (blaSHV) and virulence (vhh) genes were detected in sponge-associated isolates. The resistance gene for copper and silver (cusB) was detected in one sea urchin isolate. Plasmids were found in 11 (44%) of the isolates. This new information allows a better comprehension of antibiotic resistance in aquatic environments, since those invertebrates host resistant Vibrio spp. Thus, Vibrio associated with marine animals may pose a potential risk to public health due to carrying these antibiotic-resistant genes.
Method of Medical Equipment Evaluation and Preparation for On-Demand Additive Manufacturing with the Conventional Supply Chain Being Broken: A Case Study of Mask Filter Adapter Production during COVID-19
Authors: Karoluk Michał , Koenig Gustaw , Kurzynowski Tomasz
Published: December 2021

A year after the first cases, the COVID-19 pandemic continued to put immense pressure on healthcare systems worldwide. With supply chains broken, the only opportunity for hospitals was to turn to unconventional solutions to overcome medical equipment shortages in the shortest possible time. This paper describes the methodology that allows the preparation of medical equipment to be additionally manufactured in keeping with medical requirements. The proposed method focuses on cytotoxicity tests against HaCaT, L929, A549 cell lines, and the assessment of potential antimicrobial properties of materials. The application of the proposed method is shown by the example of an adapter that connects a full mask with widely available DAR filters. The prototyping and fabrication of adapters were conducted with the fused deposition modelling (FDM) and selective laser sintering (SLS) technologies. The adapters increased the number of masks available to medical personnel, and by the same token, ensured the continuity of their work.
Spatiotemporal Road Traffic Anomaly Detection: A Tensor-Based Approach
Authors: Tišljarić Leo , Fernandes Sofia , Carić Tonči , Gama João
Published: December 2021

The increased development of urban areas results in a larger number of vehicles on the road network, leading to traffic congestion, which often leads to potentially dangerous situations that can be described as anomalies. The tensor-based methods emerged only recently in applications related to traffic anomaly detection. They outperform other models regarding simultaneously capturing spatial and temporal components, which are of immense importance in traffic dataset analysis. This paper presents a tensor-based method for extracting the spatiotemporal road traffic patterns represented with the speed transition matrices, with the goal of anomaly detection. A novel anomaly detection approach is presented, which relies on computing the center of mass of the observed traffic patterns. The method was evaluated on a large road traffic dataset and was able to detect the most anomalous parts of the urban road network. By analyzing spatial and temporal components of the most anomalous traffic patterns, sources of anomalies can be identified. Results were validated using the extracted domain knowledge from the Highway Capacity Manual. The anomaly detection model achieved a precision score of 92.88%. Therefore, this method finds its usages for safety experts in detecting potentially dangerous road segments, urban traffic planners, and routing applications.
Flexible Vibrotactile Actuator Based on Dielectric Elastomer for Smart Handheld Devices
Authors: Heo Yong Hae , Choi Dong-Soo , Kim Do Eun , Kim Sang-Youn
Published: December 2021

This paper presents an electroactive and soft vibrotactile actuator based on a dielectric elastomer. The vibrotactile actuator is composed of an upper layer, an adhesive tape layer, a dielectric layer with bumps, and a lower layer. When a voltage is applied to the actuator, an electrostatic force created between the upper and lower layers pulls the upper layer down, compressing the dielectric layer. As soon as the applied voltage is released, the upper layer is quickly restored to its initial state by the elastic force of the compressed dielectric elastomer. Because two forces contribute to the actuation at the same time, the created vibration is sufficiently strong to stimulate human mechanoreceptors. When the applied voltage is removed, the upper layer and dielectric elastomer return to their initial shapes. We conducted experiments to determine the best weight ratio of polydimethylsiloxane (PDMS) and Ecoflex, and to quantitatively investigate the haptic performance of the proposed vibrotactile actuator. The experiments clearly show that the plasticized vibrotactile actuator can create a variety of haptic sensations over a wide frequency range.
Effects of Pause Design on the Decline in Pulling Effort and the Evaluation of Perceived Effort in Pulling Tasks
Authors: Yi Cannan , Tang Fan , Li Kaiway , Hu Hong , Zuo Huali , Zhao Caijun
Published: December 2021

Pulling is one of the manual material handling activities that could lead to work-related musculoskeletal disorders. The objectives of this study were to explore the development of muscular fatigue when performing intermittent pulling tasks and to establish models to predict the pull strength decrease due to performing the tasks. A simulated truck pulling experiment was conducted. Eleven healthy male adults participated. The participants pulled a handle with a load of 40 kg, which resulted in a pulling force of approximately 123 N. The pulling tasks lasted for 9 or 12 min with one, two, or three pauses embedded. The total time period of the embedded pauses was 3 min. The pull strength after each pull and rest was measured. Ratings of the perceived exertion on body parts after each pull were also recorded. The results showed insignificant differences regarding the development of muscular fatigue related to rest frequency. We found that the development of muscular fatigue for pulling tasks with embedded pauses was significantly slower than that for continuous pulls. The forearm had a higher CR-10 score than the other body parts indicating that the forearm was the body part suffering early muscle fatigue. An exponential model was developed to predict the pull strength of the pulling tasks with embedded pauses. This model may be used to assess the developing of muscular fatigue for pulling tasks.