bluuebunny/crossref_metadata_2025 huggingface dataset:
Genotype × Light Quality Interaction on Rose Architecture
Authors: Crespel Laurent , Le Bras Camille , Amoroso Thomas , Unda Ulloa Mateo Gabriel , Morel Philippe , Sakr Soulaiman
Published: June 2020

Plant shape, and thereby plant architecture, is a major component of the visual quality of ornamental plants. Plant architecture results from growth and branching processes and is dependent on genetic and environmental factors such as light quality. The effects of genotype and light quality and their interaction were evaluated on rose bush architecture. In a climatic growth chamber, three cultivars (Baipome, Knock Out® Radrazz and ‘The Fairy’) with contrasting architecture were exposed to three different light spectra, using white (W), red (R), and far-red (FR) light-emitting diodes (LEDs), i.e., W, WR, and WRFR. The R/FR ratio varied between treatments, ranging from 7.5 for WRFR to 23.2 for WR. Light intensity (224.6 μmol m−2 s−1) was the same for all treatments. Plants were grown up to the order 1 axis flowering stage, and their architecture was digitized at two observation scales—plant and axis. Highly significant genotype and light quality effects were revealed for most of the variables measured. An increase in stem length, in the number of axes and in the number of flowered axes was observed under the FR enriched light, WRFR. However, a strong genotype × light quality interaction, i.e., a genotype-specific response was highlighted. More in-depth eco-physiological and biochemical investigations are needed to better understand rose behavior in response to light quality and thus identify the determinants of the genotype × light quality interaction.
In Vitro Azole and Amphotericin B Susceptibilities of Malassezia furfur from Bloodstream Infections Using E-Test and CLSI Broth Microdilution Methods
Authors: Rhimi Wafa , Inyang Aneke Chioma , Mosca Adriana , Otranto Domenico , Cafarchia Claudia
Published: June 2020

The number of reports of Malassezia furfur bloodstream infections is constantly increasing and there is a need for more simple antifungal susceptibility methods for their management. In this study, a total of 39 M. furfur isolates collected from hospitalized patients with fungemia were screened for antifungal susceptibility to azole and amphotericin B (AmB) using Clinical and Laboratory Standards Institute broth microdilution (CLSI BMD) and E-test in Sabouraud dextrose agar + 1% Tween80 (SDAt) and mDixon agar (DIX). Essential agreement (EA) and discrepancies between the two methods were evaluated after 48 h and 72 h reading times. Itraconazole (ITZ) and posaconazole (POS) displayed the lowest MIC values whereas fluconazole (FLZ) and AmB the highest, regardless of the methods and the reading time. The EA between BMD was >95% for FLZ and voriconazole (VOR) regardless of the media in the E-tests and reading time. The EA between BMD with E-test for AmB was >97% only when E-test in SDAt was used. The EA between BMD and E-test for ITZ and POS varied according to the media in E-test procedures and the reading time and was higher than 66.6% (POS) or 72% (ITZ) only when SABt was used. Substantial discrepancies for ITZ and POS were >5.1% regardless of the media and the reading time. This study suggests that the E-test in SABt represents an alternative method to CLSI BMD to evaluate the susceptibility of M. furfur to FLZ, VOR and AmB and not for ITZ and POS.
Three-Dimensional Analysis of Root Anatomy and Root Canal Curvature in Mandibular Incisors Using Micro-Computed Tomography with Novel Software
Authors: Lee JongKi , Lee Shin-Hoon , Hong Jong-Rak , Kum Kee-Yeon , Oh Soram , Al-Ghamdi Adel Saeed , Al-Ghamdi Fawzi Ali , Mandorah Ayman Omar , Jang Ji-Hyun , Chang Seok Woo
Published: June 2020

Root canal treatment of mandibular incisor is difficult because of the narrow pulp space and apical curvature. The aim of this study was to measure the anatomical indicators of the mandibular incisors in Koreans using micro-computed tomography (MCT) with novel software (Kappa 2). The MCT-scanned data from 27 mandibular incisors were reconstructed and analyzed. For each canal, 3-dimensional (3D) surface models were re-sliced at 0.1 mm intervals perpendicular to the central axis of the root canal. Root canal width, dentine thickness, and direction and degree of root canal curvatures were measured automatically on each slice. Measurements were analyzed statistically with Bhapkar test, Friedman test, and Wilcoxon signed rank test. Labial and lingual dentine thicknesses were significantly larger than mesial and distal thicknesses (p < 0.001). The thinnest dentine was mainly located on the mesio-lingual side of the canals in the apical third. The mean narrowest and widest canal width in the apical sixth were 0.22 mm and 0.40 mm, respectively. The canal curvature abruptly increased in the apical 0.5-mm portion. MCT with novel software provided useful anatomical information for root canal instrumentation.
A Polarity Capturing Sphere for Word to Vector Representation
Authors: Rizkallah Sandra , Atiya Amir F. , Shaheen Samir
Published: June 2020

Embedding words from a dictionary as vectors in a space has become an active research field, due to its many uses in several natural language processing applications. Distances between the vectors should reflect the relatedness between the corresponding words. The problem with existing word embedding methods is that they often fail to distinguish between synonymous, antonymous, and unrelated word pairs. Meanwhile, polarity detection is crucial for applications such as sentiment analysis. In this work we propose an embedding approach that is designed to capture the polarity issue. The approach is based on embedding the word vectors into a sphere, whereby the dot product between any vectors represents the similarity. Vectors corresponding to synonymous words would be close to each other on the sphere, while a word and its antonym would lie at opposite poles of the sphere. The approach used to design the vectors is a simple relaxation algorithm. The proposed word embedding is successful in distinguishing between synonyms, antonyms, and unrelated word pairs. It achieves results that are better than those of some of the state-of-the-art techniques and competes well with the others.
Recent Trends in DC and Hybrid Microgrids: Opportunities from Renewables Sources, Battery Energy Storages and Bi-Directional Converters
Authors: Saponara Sergio , Saletti Roberto , Mihet-Popa Lucian
Published: June 2020

This editorial manuscript reviews the papers accepted for publication in the Special Issue “DC & Hybrid Microgrids” of Applied Sciences. This Special Issue, co-organized by the University of Pisa, Italy and Østfold University College in Norway, has collected nine papers from 25 submitted, with authors from Asia, North America and Europe. The published articles provide an overview of the most recent research advances in direct current (DC) and hybrid microgrids, exploiting the opportunities offered by the use of renewable energy sources, battery energy storage systems, power converters, innovative control and energy management strategies.
Assessment of Angular Spectral Distributions of Laser Accelerated Particles for Simulation of Radiation Dose Map in Target Normal Sheath Acceleration Regime of High Power Laser-Thin Solid Target Interaction—Comparison with Experiments
Authors: Groza Andreea , Chirosca Alecsandru , Stancu Elena , Butoi Bogdan , Serbanescu Mihai , Dreghici Dragana B. , Ganciu Mihai
Published: June 2020

An adequate simulation model has been used for the calculation of angular and energy distributions of electrons, protons, and photons emitted during a high-power laser, 5-µm thick Ag target interaction. Their energy spectra and fluencies have been calculated between 0 and 360 degrees around the interaction point with a step angle of five degrees. Thus, the contribution of each ionizing species to the total fluency value has been established. Considering the geometry of the experimental set-up, a map of the radiation dose inside the target vacuum chamber has been simulated, using the Geant4 General Particle Source code, and further compared with the experimental one. Maximum values of the measured dose of the order of tens of mGy per laser shot have been obtained in the direction normal to the target at about 30 cm from the interaction point.
Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches
Authors: Víquez Luis , Barquero Vinicio , Soler Carles , Roldan Eduardo R.S. , Valverde Anthony
Published: June 2020

The ejaculate is heterogenous and sperm sub-populations with different kinematic patterns can be identified in various species. Nevertheless, although these sub-populations are statistically well defined, the statistical differences are not always relevant. The aim of the present study was to characterize kinematic sub-populations in sperm from two bovine species, and diluted with different commercial extenders, and to determine the statistical relevance of sub-populations through Bayesian analysis. Semen from 10 bulls was evaluated after thawing. An ISAS®v1 computer-assisted sperm analysis (CASA)-Mot system was employed with an image acquisition rate of 50 Hz and ISAS®D4C20 counting chambers. Sub-populations of motile spermatozoa were characterized using multivariate procedures such as principal components (PCs) analysis and clustering methods (k-means model). Four different sperm sub-populations were identified from three PCs that involved progressiveness, velocity, and cell undulatory movement. The proportions of the different sperm sub-populations varied with the extender used and in the two species. Despite a statistical difference (p < 0.05) between extenders, the Bayesian analysis confirmed that only one of them (Triladyl®) presented relevant differences in kinematic patterns when compared with Tris-EY and OptiXcell®. Extenders differed in the proportion of sperm cells in each of the kinematic sub-populations. Similar patterns were identified in Bos taurus and Bos indicus. Bayesian results indicate that sub-populations SP1, SP2, and SP3 were different for PC criteria and these differences were relevant. For velocity, linearity, and progressiveness, the SP4 did not show a relevant difference regarding the other sperm sub-populations. The classical approach of clustering or sperm subpopulation thus may not have a direct biological meaning. Therefore, the biological relevance of sperm sub-populations needs to be reevaluated.
Molecular Characterization of Astrocytoma Progression Towards Secondary Glioblastomas Utilizing Patient-Matched Tumor Pairs
Authors: Seifert Michael , Schackert Gabriele , Temme Achim , Schröck Evelin , Deutsch Andreas , Klink Barbara
Published: June 2020

Astrocytomas are primary human brain tumors including diffuse or anaplastic astrocytomas that develop towards secondary glioblastomas over time. However, only little is known about molecular alterations that drive this progression. We measured multi-omics profiles of patient-matched astrocytoma pairs of initial and recurrent tumors from 22 patients to identify molecular alterations associated with tumor progression. Gene copy number profiles formed three major subcluters, but more than half of the patient-matched astrocytoma pairs differed in their gene copy number profiles like astrocytomas from different patients. Chromosome 10 deletions were not observed for diffuse astrocytomas, but occurred in corresponding recurrent tumors. Gene expression profiles formed three other major subclusters and patient-matched expression profiles were much more heterogeneous than their copy number profiles. Still, recurrent tumors showed a strong tendency to switch to the mesenchymal subtype. The direct progression of diffuse astrocytomas to secondary glioblastomas showed the largest number of transcriptional changes. Astrocytoma progression groups were further distinguished by signaling pathway expression signatures affecting cell division, interaction and differentiation. As expected, IDH1 was most frequently mutated closely followed by TP53, but also MUC4 involved in the regulation of apoptosis and proliferation was frequently mutated. Astrocytoma progression groups differed in their mutation frequencies of these three genes. Overall, patient-matched astrocytomas can differ substantially within and between patients, but still molecular signatures associated with the progression to secondary glioblastomas exist and should be analyzed for their potential clinical relevance in future studies.
Influence of Fibroblasts on Mammary Gland Development, Breast Cancer Microenvironment Remodeling, and Cancer Cell Dissemination
Authors: Avagliano Angelica , Fiume Giuseppe , Ruocco Maria Rosaria , Martucci Nunzia , Vecchio Eleonora , Insabato Luigi , Russo Daniela , Accurso Antonello , Masone Stefania , Montagnani Stefania , Arcucci Alessandro
Published: June 2020

The stromal microenvironment regulates mammary gland development and tumorigenesis. In normal mammary glands, the stromal microenvironment encompasses the ducts and contains fibroblasts, the main regulators of branching morphogenesis. Understanding the way fibroblast signaling pathways regulate mammary gland development may offer insights into the mechanisms of breast cancer (BC) biology. In fact, the unregulated mammary fibroblast signaling pathways, associated with alterations in extracellular matrix (ECM) remodeling and branching morphogenesis, drive breast cancer microenvironment (BCM) remodeling and cancer growth. The BCM comprises a very heterogeneous tissue containing non-cancer stromal cells, namely, breast cancer-associated fibroblasts (BCAFs), which represent most of the tumor mass. Moreover, the different components of the BCM highly interact with cancer cells, thereby generating a tightly intertwined network. In particular, BC cells activate recruited normal fibroblasts in BCAFs, which, in turn, promote BCM remodeling and metastasis. Thus, comparing the roles of normal fibroblasts and BCAFs in the physiological and metastatic processes, could provide a deeper understanding of the signaling pathways regulating BC dissemination. Here, we review the latest literature describing the structure of the mammary gland and the BCM and summarize the influence of epithelial-mesenchymal transition (EpMT) and autophagy in BC dissemination. Finally, we discuss the roles of fibroblasts and BCAFs in mammary gland development and BCM remodeling, respectively.
Switchable Graphene-Based Bioelectronics Interfaces
Authors: Meenakshi  , Shukla Sudheesh , Narang Jagriti , Kumar Vinod , Govender Penny , Niv Avi , Hussain Chaudhery , Wang Rui , Mangla Bindu , Babu Rajendran
Published: June 2020

Integration of materials acts as a bridge between the electronic and biological worlds, which has revolutionized the development of bioelectronic devices. This review highlights the rapidly emerging field of switchable interface and its bioelectronics applications. This review article highlights the role and importance of two-dimensional (2D) materials, especially graphene, in the field of bioelectronics. Because of the excellent electrical, optical, and mechanical properties graphene have promising application in the field of bioelectronics. The easy integration, biocompatibility, mechanical flexibility, and conformity add impact in its use for the fabrication of bioelectronic devices. In addition, the switchable behavior of this material adds an impact on the study of natural biochemical processes. In general, the behavior of the interfacial materials can be tuned with modest changes in the bioelectronics interface systems. It is also believed that switchable behavior of materials responds to a major change at the nanoscale level by regulating the behavior of the stimuli-responsive interface architecture.
Effects of NiSO4 Concentration on the Coloring Performance and Corrosion Resistance of the Colored Film on 304 Stainless Steel
Authors: Li Wenwei , Qu Jun-e , Cao Zhiyong , Wang Hairen
Published: June 2020

The colored films were successfully prepared on the 304 stainless steel surfaces in coloring solutions with different NiSO4 contents. The purpose of this study was to investigate the effects of NiSO4 in the coloring solution on the coloring performance of 304 stainless steel and corrosion resistance of the obtained colored film in NaCl solution. The coloring rate was determined from coloring potential-time curve, and the protection properties of the color films in a 3.5% NaCl solution were characterized by potentiodynamic polarization scan, electrochemical impedance spectroscopy, and wear resistance test. The results showed that adding NiSO4 could accelerate the coloring process but brought about a negative impact on the surface’s corrosion resistance.
Experimental Observation of Flow Reversal in Thin Liquid Film Flow Falling on an Inclined Plate
Authors: Wang Ruiqi , Jia Haijun , Duan Riqiang
Published: June 2020

A customized particle image velocimetry/planar laser induced fluorescence (PIV/PLIF) experimental method, aiming to capture transient hydrodynamics of solitary waves of inertia-dominated falling liquid films, is presented in this paper. A novel PIV/PLIF technique, which only uses one camera to capture simultaneously both particle image and fluorescence, and meanwhile a post-processing imaging method is also developed, which is able to simultaneously measure both internal velocity field in film and its topology. To validate the fidelity of the novel PIV/PLIF technique, a comparison between experimental results of streamwise velocity profile and film thickness and that of the Nusselt’s prediction at low Reynold number is carried out, and in addition, integral continuity is checked for transient wavy film, both of which shows that they are in good agreement. Based on experimental velocity fields and film topology, pressure distribution inside film is derived with the Poisson equation. Considering characteristics of traveling waves, the experimental results are presented respectively in spatial mode and temporal mode. In spatial mode, capillary wave dynamics are demonstrated out of velocity field, film topology and pressure distribution, which reveals that flow reversal occurs at capillary troughs. In temporal mode, the mechanism of flow reversal at capillary troughs is scrutinized on the basis of high-frequency velocity sampling and the derived pressure gradient. It is shown that flow reversal at capillary troughs is triggered due to occurrence of positive pressure gradient at the back side of the capillary wave crest, rather than the trough upstream as stated by the previous researchers. By elucidating the dynamics of flow reversal, mechanisms for the upper limit of Reynold number with respect to flow reversal underneath capillary wave trough were proposed, which might be the gradually saturated deceleration from the capillary curvature and shorten deceleration duration determined by the wave speed and capillary wave length. Our results should be of interest for optimization of the mass transport model of falling liquid films and shed light on the revealing of flow reversal mechanism.
Dynamics of Radiation Damage in AlN Ceramics under High-Dose Irradiation, Typical for the Processes of Swelling and Hydrogenation
Authors: Kozlovskiy Artem L. , Shlimas Dmitriy. I. , Kenzhina Inesh E. , Borgekov Daryn B. , Zdorovets Maxim V.
Published: June 2020

The use of nitride ceramics, in particular AlN, as structural materials for nuclear power is primarily limited by their resistance to swelling and hydrogenation processes due to the accumulation of poorly soluble helium and hydrogen ions in the structure of the surface layer. In this regard, research in this area is of great importance not only from a fundamental point of view, but also practical, since any new data on radiation resistance can make a great contribution to the development of the theory of resistance to radiation influences of structural materials of a new generation. This work is devoted to a systematic study and comparative analysis of the dynamics of radiation damage during high-dose irradiation with protons and helium ions in nitride ceramics, which have great potential for use as structural materials for GenIV reactors. The choice of irradiation doses of 1 × 1017–5 × 1017 ion/cm2 is due to the possibility of modeling the processes of radiation damage characteristic of displacements of 10–50 dpa. During the study, the dependences of the change in the dielectric and conductive characteristics of nitride ceramics depending on the radiation dose, as well as on the type of ions, were established. The kinetics of degradation and accelerated aging was determined depending on the type of exposure. The mechanical and strength properties of ceramics were determined.
Colossal Negative Magnetoresistance Effect in a La1.37Sr1.63Mn2O7 Single Crystal Grown by Laser-Diode-Heated Floating-Zone Technique
Authors: Wu Si , Zhu Yinghao , Xia Junchao , Zhou Pengfei , Ni Haiyong , Li Hai-Feng
Published: June 2020

We have grown La 1.37 Sr 1.63 Mn 2 O 7 single crystals with a laser-diode-heated floating-zone furnace and studied the crystallinity, structure, and magnetoresistance (MR) effect by in-house X-ray Laue diffraction, X-ray powder diffraction, and resistance measurements. The La 1.37 Sr 1.63 Mn 2 O 7 single crystal crystallizes into a tetragonal structure with space group I4/mmm at room temperature. At 0 T, the maximum resistance centers around ∼166.9 K. Below ∼35.8 K, it displays an insulating character with an increase in resistance upon cooling. An applied magnetic field of B = 7 T strongly suppresses the resistance indicative of a negative MR effect. The minimum MR value equals −91.23% at 7 T and 128.7 K. The magnetic-field-dependent resistance shows distinct features at 1.67, 140, and 322 K, from which we calculated the corresponding MR values. At 14 T and 140 K, the colossal negative MR value is down to −94.04(5)%. We schematically fit the MR values with different models for an ideal describing of the interesting features of the MR value versus B curves.
Unexpected Selective Gas Adsorption on a ‘Non-Porous’ Metal Organic Framework
Authors: Beveridge Stuart , McAnally Craig A. , Nichol Gary S. , Kennedy Alan R. , Cussen Edmund J. , Fletcher Ashleigh J.
Published: June 2020

A metal organic framework Cu(tpt)BF4·¾H2O was synthesized as a potential carbon capture material, with the aim being to exploit the Lewis base interaction of the incorporated ligand functionalities with acidic gas. The material displays high thermal stability but an exceptionally low surface area; however, this contrasts starkly with its ability to capture carbon dioxide, demonstrating significant activated diffusion within the framework. The full characterization of the material shows a robust structure, where the CO2 sorption is 120% greater than current industrial methods using liquid amine solutions; the thermal energy required for sorbent regeneration is reduced by 65%, indicating the true industrial potential of the synthesized material.
Dairy Fat and Cardiovascular Health
Authors: Sendra Esther
Published: June 2020

Current scientific evidence points to a neutral or positive effect of dairy fats intake on cardiovascular health. After years of controversy, with many guidelines recommending a reduced intake of dairy products, and preferably low or nonfat dairy foods, current knowledge points to the more appropriate recommendation of moderate consumption of full-fat dairy foods within a healthy lifestyle. Fermented dairy products seem to be the best option as a source of nutrients and cardiovascular health benefits. Previous recommendations were based on cholesterol, saturated fat, and caloric contents, in dairy fat, and their potential impact on serum cholesterol, fasting sugar levels, and blood pressure. However, experimental data point to a more complex scenario in which other actors may play major roles: calcium, bioactive lipids and peptides, and even the food-matrix effect from the dairy food side, and human genetics and environmental factors all impact dairy food-related health issues. Furthermore, cardiovascular health does not rely solely on serum cholesterol levels and blood pressure but also on inflammatory biomarkers. At present, little is known on the true mechanisms underlying the cardioprotective mechanism of dairy fats, and further research in needed to elucidate them.
Therapeutic Editing of the TP53 Gene: Is CRISPR/Cas9 an Option?
Authors: Mirgayazova Regina , Khadiullina Raniya , Chasov Vitaly , Mingaleeva Rimma , Miftakhova Regina , Rizvanov Albert , Bulatov Emil
Published: June 2020

The TP53 gene encodes the transcription factor and oncosuppressor p53 protein that regulates a multitude of intracellular metabolic pathways involved in DNA damage repair, cell cycle arrest, apoptosis, and senescence. In many cases, alterations (e.g., mutations of the TP53 gene) negatively affect these pathways resulting in tumor development. Recent advances in genome manipulation technologies, CRISPR/Cas9, in particular, brought us closer to therapeutic gene editing for the treatment of cancer and hereditary diseases. Genome-editing therapies for blood disorders, blindness, and cancer are currently being evaluated in clinical trials. Eventually CRISPR/Cas9 technology is expected to target TP53 as the most mutated gene in all types of cancers. A majority of TP53 mutations are missense which brings immense opportunities for the CRISPR/Cas9 system that has been successfully used for correcting single nucleotides in various models, both in vitro and in vivo. In this review, we highlight the recent clinical applications of CRISPR/Cas9 technology for therapeutic genome editing and discuss its perspectives for editing TP53 and regulating transcription of p53 pathway genes.
PPARG Pro12Ala Polymorphism with CKD in Asians: A Meta-Analysis Combined with a Case-Control Study—A Key for Reaching Null Association
Authors: Chen Hsiang-Cheng , Chen Wei-Teing , Sung Tzu-Ling , Tsai Dung-Jang , Lin Chin , Su Hao , Lin Yuh-Feng , Chiu Hung-Yi , Su Sui-Lung
Published: June 2020

Background: So far, numerous meta-analyses have been published regarding the correlation between peroxisome proliferator-activated receptor gamma (PPARG) proline 12 alanine (Pro12Ala) gene polymorphism and chronic kidney disease (CKD); however, the results appear to be contradictory. Hence, this study is formulated with the objective of using existing meta-analysis data together with our research population to study the correlation between PPARG Pro12Ala gene polymorphism and CKD and evaluate whether an accurate result can be obtained. Methods: First, literature related to CKD and PPARG Pro12Ala available on the PubMed and EMBASE databases up to December 2016 was gathered from 20 publications. Then, the gathered results were combined with our case-control study of 1693 enrolled subjects and a trial sequential analysis (TSA) was performed to verify existing evidence and determine whether a firm conclusion can be drawn. Results: The TSA results showed that the cumulative sample size for the Asian sample was 6078 and was sufficient to support a definite result. The results of this study confirmed that there is no obvious correlation between PPARG Pro12Ala and CKD for Asians (OR = 0.82 (95% CI = 0.66–1.02), I2 = 63.1%), but this was not confirmed for Caucasians. Furthermore, the case-control sample in our study was shown to be the key for reaching this conclusion. Conclusions: The meta-analysis results of this study suggest no significant correlation between PPARG Pro12Ala gene polymorphism and CKD for Asians after adding our samples, but not for Caucasian.
A Private 16q24.2q24.3 Microduplication in a Boy with Intellectual Disability, Speech Delay and Mild Dysmorphic Features
Authors: Palumbo Orazio , Palumbo Pietro , Di Muro Ester , Cinque Luigia , Petracca Antonio , Carella Massimo , Castori Marco
Published: June 2020

No data on interstitial microduplications of the 16q24.2q24.3 chromosome region are available in the medical literature and remain extraordinarily rare in public databases. Here, we describe a boy with a de novo 16q24.2q24.3 microduplication at the Single Nucleotide Polymorphism (SNP)-array analysis spanning ~2.2 Mb and encompassing 38 genes. The patient showed mild-to-moderate intellectual disability, speech delay and mild dysmorphic features. In DECIPHER, we found six individuals carrying a “pure” overlapping microduplication. Although available data are very limited, genomic and phenotype comparison of our and previously annotated patients suggested a potential clinical relevance for 16q24.2q24.3 microduplication with a variable and not (yet) recognizable phenotype predominantly affecting cognition. Comparing the cytogenomic data of available individuals allowed us to delineate the smallest region of overlap involving 14 genes. Accordingly, we propose ANKRD11, CDH15, and CTU2 as candidate genes for explaining the related neurodevelopmental manifestations shared by these patients. To the best of our knowledge, this is the first time that a clinical and molecular comparison among patients with overlapping 16q24.2q24.3 microduplication has been done. This study broadens our knowledge of the phenotypic consequences of 16q24.2q24.3 microduplication, providing supporting evidence of an emerging syndrome.
Assessing the Implementation of a Behavior Change Intervention: Process Evaluation of a Stepped-Wedge Cluster Randomized Controlled Trial for Newborn Health
Authors: Bazzano Alessandra N. , Stolow Jeni A. , Duggal Ryan , Oberhelman Richard A. , Sun Yaoyao , Var Chivorn
Published: June 2020

Maternal and under five-year-old mortality rates have reduced in the last two decades globally due to concerted effort, yet newborn deaths remain unacceptably prevalent. Behavior change is an important component of interventions to address newborn health problems in low-income countries. In Cambodia, maternal and newborn mortality has markedly decreased, and continued improvements will allow the country to achieve further reduction in newborn morbidity. The results of an implementation study of the Newborn Care and Infection Control Initiative using process evaluation are presented to provide insight into the trial implementation and context of the program that may have contributed to intervention results. The study utilized a mixed method process to explore the context, mechanisms, and implementation of intervention components: training of village health support group volunteers to provide home visits, training of midwives on infection prevention and control around the perinatal period, counseling on newborn care, and provision of training materials for counseling and intervention. Implementation was evaluated through quantitative and qualitative data collection including surveys, observation, semi-structured interviews, focus groups, and visual media. Descriptive statistics summarized the quantitative data and thematic analysis was used to explore the qualitative data. The evaluation identified several factors that might have influenced the outcomes of the trial: continuity of health center staff communication, timing and ability to complete home visits, and training quality. Additional support for parents in the perinatal period, preferably provided at the community level, will contribute to further improvement in health outcomes for newborns in this area. Researchers in this context should consider mechanisms to improve the coordination of health facility staff counseling while providing support and resources to ensure home visits to families with newborns are made on time. Attention to staffing, training, and quality of newborn health interventions is critical in planning for the scaling-up of newborn health programming.
Interpersonal Violence and Psychotic-Like Experiences: The Mediation of Ideas of Reference, Childhood Memories, and Dissociation
Authors: Fernández-León Sandra , Rodríguez-Testal Juan F. , Gutiérrez-López María L. , Senín-Calderón Cristina
Published: June 2020

Previous studies have demonstrated the relationship between the accumulation of situations involving interpersonal violence (IV) and psychotic-like experiences. This study explored whether IV is related to aberrant salience (AS), using a sequential mediation model that included memories of relationship with parents (submission, devaluation, and threat; Early Life Experiences Scale (ELES)), ideas of reference (IR), and dissociative symptoms (absorption and depersonalization), and whether the patient/nonpatient condition moderated this effect. The sample was made of 401 participants (including 43 patients with psychotic disorders) aged 18 to 71 years (Mage = 30.43; SD = 11.19). Analysis of a serial multiple mediator model revealed that IR, ELES, absorption, and depersonalization fully mediated the effect of IV on AS, explaining 39% of the variance, regardless of the patient/nonpatient condition. The indirect paths, which place IR and dissociation (especially absorption, the variable to which the IR and ELES lead) in a primordial position for being related to AS, are discussed. This continuum model could be useful for understanding processes related to the onset of psychosis unmoderated by the patient/nonpatient condition.
Impact of Apartment Tightness on Temperature Variability during a Fire
Authors: Gałaj Jerzy , Saleta Damian
Published: June 2020

Along with the thermal modernization process of old residential buildings, there has been a significant increase in the air tightness of apartments, which may contribute to the deterioration of the safety of users and rescue teams in a fire. The main goal of this study was to investigate the impact of the air tightness of an apartment on fire growth and temperature variability. In the work, an experimental method was applied. Two full-scale fire tests were carried out, one in a sealed apartment and the other in unsealed one. The temperature was measured by thirty-two thermocouples. Two thermal imaging and video cameras were also used to evaluate a temperature field as well as flame and smoke height. Based on the analysis, conclusions have been formulated. It is noteworthy that the highest temperatures and significant increase in pressure were obtained in a sealed apartment, but dangerous and critical conditions regarding firefighters’ safety were achieved faster and persisted much longer in an unsealed one.
Experiment in Finding Look-Alike European Cities Using Urban Atlas Data
Authors: Dobesova Zdena
Published: June 2020

The integration of geography and machine learning can produce novel approaches in addressing a variety of problems occurring in natural and human environments. This article presents an experiment that identifies cities that are similar according to their land use data. The article presents interesting preliminary experiments with screenshots of maps from the Czech map portal. After successfully working with the map samples, the study focuses on identifying cities with similar land use structures. The Copernicus European Urban Atlas 2012 was used as a source dataset (data valid years 2015–2018). The Urban Atlas freely offers land use datasets of nearly 800 functional urban areas in Europe. To search for similar cities, a set of maps detailing land use in European cities was prepared in ArcGIS. A vector of image descriptors for each map was subsequently produced using a pre-trained neural network, known as Painters, in Orange software. As a typical data mining task, the nearest neighbor function analyzes these descriptors according to land use patterns to find look-alike cities. Example city pairs based on land use are also presented in this article. The research question is whether the existing pre-trained neural network outside cartography is applicable for categorization of some thematic maps with data mining tasks such as clustering, similarity, and finding the nearest neighbor. The article’s contribution is a presentation of one possible method to find cities similar to each other according to their land use patterns, structures, and shapes. Some of the findings were surprising, and without machine learning, could not have been evident through human visual investigation alone.
Tocotrienols Influence Body Weight Gain and Brain Protein Expression in Long-Term High-Fat Diet-Treated Mice
Authors: Kato Yugo , Aoki Yoshinori , Fukui Koji
Published: June 2020

Obesity induces serious diseases such as diabetes and cardiovascular disease. It has been reported that obesity increases the risk of cognitive dysfunction. Cognitive dysfunction is a characteristic symptom of Alzheimer’s and Parkinson’s diseases. However, the detailed mechanisms of obesity-induced cognitive dysfunction have not yet been elucidated. The onset and progression of obesity-induced severe secondary diseases such as diabetes, cardiovascular events, and hypertension are deeply connected to oxidative stress. We hypothesized that obesity induces cognitive dysfunction via acceleration of reactive oxygen species (ROS) production. Vitamin E, which is a lipophilic vitamin, has strong antioxidative effects and consists of two groups: tocopherols and tocotrienols. Recently, it has been demonstrated that tocotrienols have strong neuroprotective and anti-obesity effects. In this study, we fed mice a high-fat diet (HFD) from 9 to 14 months of age and assessed the effect of tocotrienols treatment on body weight, brain oxidation levels, and cognitive function. The results revealed that treatment with tocotrienols inhibited body weight gain; further, tocotrienols reached the brain and attenuated oxidation in HFD-treated mice. These results indicate that tocotrienols have anti-obesity effects and inhibit obesity-induced brain oxidation.
Trefoil Factor Family (TFF) Peptides and Their Diverse Molecular Functions in Mucus Barrier Protection and More: Changing the Paradigm
Authors: Hoffmann Werner
Published: June 2020

Trefoil factor family peptides (TFF1, TFF2, TFF3) are typically co-secreted together with mucins. Tff1 represents a gastric tumor suppressor gene in mice. TFFs are also synthesized in minute amounts in the immune and central nervous systems. In mucous epithelia, they support rapid repair by enhancing cell migration (“restitution”) via their weak chemotactic and anti-apoptotic effects. For a long time, as a paradigm, this was considered as their major biological function. Within recent years, the formation of disulfide-linked heterodimers was documented for TFF1 and TFF3, e.g., with gastrokine-2 and IgG Fc binding protein (FCGBP). Furthermore, lectin activities were recognized as enabling binding to a lipopolysaccharide of Helicobacter pylori (TFF1, TFF3) or to a carbohydrate moiety of the mucin MUC6 (TFF2). Only recently, gastric TFF1 was demonstrated to occur predominantly in monomeric forms with an unusual free thiol group. Thus, a new picture emerged, pointing to diverse molecular functions for TFFs. Monomeric TFF1 might protect the gastric mucosa as a scavenger for extracellular reactive oxygen/nitrogen species. Whereas, the TFF2/MUC6 complex stabilizes the inner layer of the gastric mucus. In contrast, the TFF3–FCGBP heterodimer (and also TFF1–FCGBP) are likely part of the innate immune defense of mucous epithelia, preventing the infiltration of microorganisms.
Powdery Mildew-Induced Hormonal and Photosynthetic Changes in Barley Near Isogenic Lines Carrying Various Resistant Genes
Authors: Saja Diana , Janeczko Anna , Barna Balázs , Skoczowski Andrzej , Dziurka Michał , Kornaś Andrzej , Gullner Gábor
Published: June 2020

The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes (Mla, Mlg and mlo). After inoculation with Blumeria graminis f. sp. hordei (Bgh), measurements of leaf reflectance and chlorophyll a fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi. Bgh-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.ABS) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll a fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.
X-ray Micro-Computed Tomography: An Emerging Technology to Analyze Vascular Calcification in Animal Models
Authors: Borland Samantha J. , Behnsen Julia , Ashton Nick , Francis Sheila E. , Brennan Keith , Sherratt Michael J. , Withers Philip J. , Canfield Ann E.
Published: June 2020

Vascular calcification describes the formation of mineralized tissue within the blood vessel wall, and it is highly associated with increased cardiovascular morbidity and mortality in patients with chronic kidney disease, diabetes, and atherosclerosis. In this article, we briefly review different rodent models used to study vascular calcification in vivo, and critically assess the strengths and weaknesses of the current techniques used to analyze and quantify calcification in these models, namely 2-D histology and the o-cresolphthalein assay. In light of this, we examine X-ray micro-computed tomography (µCT) as an emerging complementary tool for the analysis of vascular calcification in animal models. We demonstrate that this non-destructive technique allows us to simultaneously quantify and localize calcification in an intact vessel in 3-D, and we consider recent advances in µCT sample preparation techniques. This review also discusses the potential to combine 3-D µCT analyses with subsequent 2-D histological, immunohistochemical, and proteomic approaches in correlative microscopy workflows to obtain rich, multifaceted information on calcification volume, calcification load, and signaling mechanisms from within the same arterial segment. In conclusion we briefly discuss the potential use of µCT to visualize and measure vascular calcification in vivo in real-time.
HDAC8 Inhibition Reduces Lesional Iba-1+ Cell Infiltration after Spinal Cord Injury without Effects on Functional Recovery
Authors: Hendrix Sven , Sanchez Selien , Ventriglia Elissia , Lemmens Stefanie
Published: June 2020

Pan-histone deacetylase (HDAC) inhibition with valproic acid (VPA) has beneficial effects after spinal cord injury (SCI), although with side effects. We focused on specific HDAC8 inhibition, because it is known to reduce anti-inflammatory mediators produced by macrophages (Mφ). We hypothesized that HDAC8 inhibition improves functional recovery after SCI by reducing pro-inflammatory classically activated Mφ. Specific HDAC8 inhibition with PCI-34051 reduced the numbers of perilesional Mφ as measured by histological analyses, but did not improve functional recovery (Basso Mouse Scale). We could not reproduce the published improvement of functional recovery described in contusion SCI models using VPA in our T-cut hemisection SCI model. The presence of spared fibers might be the underlying reason for the conflicting data in different SCI models.
Understanding the Biology of Human Interstitial Cells of Cajal in Gastrointestinal Motility
Authors: Foong Daphne , Zhou Jerry , Zarrouk Ali , Ho Vincent , O’Connor Michael D.
Published: June 2020

Millions of patients worldwide suffer from gastrointestinal (GI) motility disorders such as gastroparesis. These disorders typically include debilitating symptoms, such as chronic nausea and vomiting. As no cures are currently available, clinical care is limited to symptom management, while the underlying causes of impaired GI motility remain unaddressed. The efficient movement of contents through the GI tract is facilitated by peristalsis. These rhythmic slow waves of GI muscle contraction are mediated by several cell types, including smooth muscle cells, enteric neurons, telocytes, and specialised gut pacemaker cells called interstitial cells of Cajal (ICC). As ICC dysfunction or loss has been implicated in several GI motility disorders, ICC represent a potentially valuable therapeutic target. Due to their availability, murine ICC have been extensively studied at the molecular level using both normal and diseased GI tissue. In contrast, relatively little is known about the biology of human ICC or their involvement in GI disease pathogenesis. Here, we demonstrate human gastric tissue as a source of primary human cells with ICC phenotype. Further characterisation of these cells will provide new insights into human GI biology, with the potential for developing novel therapies to address the fundamental causes of GI dysmotility.
Platelets in Healthy and Disease States: From Biomarkers Discovery to Drug Targets Identification by Proteomics
Authors: Gianazza Erica , Brioschi Maura , Baetta Roberta , Mallia Alice , Banfi Cristina , Tremoli Elena
Published: June 2020

Platelets are a heterogeneous small anucleate blood cell population with a central role both in physiological haemostasis and in pathological states, spanning from thrombosis to inflammation, and cancer. Recent advances in proteomic studies provided additional important information concerning the platelet biology and the response of platelets to several pathophysiological pathways. Platelets circulate systemically and can be easily isolated from human samples, making proteomic application very interesting for characterizing the complexity of platelet functions in health and disease as well as for identifying and quantifying potential platelet proteins as biomarkers and novel antiplatelet therapeutic targets. To date, the highly dynamic protein content of platelets has been studied in resting and activated platelets, and several subproteomes have been characterized including platelet-derived microparticles, platelet granules, platelet releasates, platelet membrane proteins, and specific platelet post-translational modifications. In this review, a critical overview is provided on principal platelet proteomic studies focused on platelet biology from signaling to granules content, platelet proteome changes in several diseases, and the impact of drugs on platelet functions. Moreover, recent advances in quantitative platelet proteomics are discussed, emphasizing the importance of targeted quantification methods for more precise, robust and accurate quantification of selected proteins, which might be used as biomarkers for disease diagnosis, prognosis and therapy, and their strong clinical impact in the near future.
Genetic Deletion of Vasohibin-2 Exacerbates Ischemia-Reperfusion-Induced Acute Kidney Injury
Authors: Miyake Hiromasa , Tanabe Katsuyuki , Tanimura Satoshi , Nakashima Yuri , Morioka Tomoyo , Masuda Kana , Sugiyama Hitoshi , Sato Yasufumi , Wada Jun
Published: June 2020

Acute kidney injury (AKI) has been increasingly recognized as a risk factor for transition to chronic kidney disease. Recent evidence suggests that endothelial damage in peritubular capillaries can accelerate the progression of renal injury. Vasohibin-2 (VASH2) is a novel proangiogenic factor that promotes tumor angiogenesis. However, the pathophysiological roles of VASH2 in kidney diseases remain unknown. In the present study, we examined the effects of VASH2 deficiency on the progression of ischemia–reperfusion (I/R) injury-induced AKI. I/R injury was induced by bilaterally clamping renal pedicles for 25 min in male wild-type (WT) and Vash2 homozygous knockout mice. Twenty-four hours later, I/R injury-induced renal dysfunction and tubular damage were more severe in VASH2-deficient mice than in WT mice, with more prominent neutrophil infiltration and peritubular capillary loss. After induction of I/R injury, VASH2 expression was markedly increased in injured renal tubules. These results suggest that VASH2 expression in renal tubular epithelial cells might be essential for alleviating I/R injury-induced AKI, probably through protecting peritubular capillaries and preventing inflammatory infiltration.