bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Melt Pool Boundary Network on the Anisotropic Mechanical Properties of Selective Laser Melted 304L
Authors: Spratt Myranda , Newkirk Joseph W. , Fashanu Okanmisope , Chandrashekhara K.
Published: October 2021

Anisotropic mechanical properties are a well-known issue in selective laser melted parts. The microstructure produced by selective laser melting (SLM) is directional, including the solidified melt pool structures and grains. This work investigates the melt pool boundary’s effects on 304L stainless steel’s compressive properties. 304L stainless steel solid cylinders were built using a pulse laser SLM machine in four directions using three hatch angle rotations: 0°, 67°, and 105°. The twelve samples were compression tested, and the results were analyzed. Numerical models were also created with the different hatch angles and directions. The melt pool boundary network (MPBN) in each build was tracked using the model across multiple planes. Results showed that both the hatch angle and build orientation influenced the concentration of melt pool boundaries present in the manufactured samples. A weak negative correlation of compressive strength to the melt pool boundaries’ concentration was also observed, indicating that the melt pool boundary concentration negatively affected the material’s strength. Local anisotropic plastic deformation was also observed in some of the compressed samples. In those samples, it was observed that directions that plastically deformed more also contained higher concentration of the melt pool boundaries.
A Vector Wavenumber Integration Model of Underwater Acoustic Propagation Based on the Matched Interface and Boundary Method
Authors: Liu Wei , Zhang Lilun , Wang Yongxian , Cheng Xinghua , Xiao Wenbin
Published: October 2021

Acoustic particle velocities can provide additional energy flow information of the sound field; thus, the vector acoustic model is attracting increasing attention. In the current study, a vector wavenumber integration (VWI) model was established to provide benchmark solutions of ocean acoustic propagation. The depth-separated wave equation was solved using finite difference (FD) methods with second- and fourth-order accuracy, and the sound source singularity in this equation was treated using the matched interface and boundary method. Moreover, the particle velocity was calculated using the wavenumber integration method, consistent with the calculation of the sound pressure. Furthermore, the VWI model was verified using acoustic test cases of the free acoustic field, the ideal fluid waveguide, the Bucker waveguide, and the Munk waveguide by comparing the solutions of the VWI model, the analytical formula, and the image method. In the free acoustic field case, the errors of the second- and fourth-order FD schemes for solving the depth-separated equation were calculated, and the actual orders of accuracy of the FD schemes were tested. Moreover, the time-averaged sound intensity (TASI) was calculated using the pressure and particle velocity, and the TASI streamlines were traced to visualize the time-independent energy flow in the acoustic field and better understand the distribution of the acoustic transmission loss.
Specification of the Okadaic Acid Equivalent for Okadaic Acid, Dinophysistoxin-1, and Dinophysistoxin-2 Based on Protein Phosphatase 2A Inhibition and Cytotoxicity Assays Using Neuro 2A Cell Line
Authors: Ikehara Tsuyoshi , Chikanishi Kazuya , Oshiro Naomasa
Published: October 2021

Diarrhetic shellfish poisoning (DSP) is a globally occurring disease threatening public health and trade. The causative toxins, okadaic acid (OA), dinophysistoxin-1 (DTX1), and dinophysistoxin-2 (DTX2) are collectively called OAs, and are quantified using the LC-MS/MS method. The hazardous effect of total OAs is expressed as the sum of OA equivalents defined for respective OAs based on mouse lethality, produced by either intraperitoneal (OAip) or oral administration (OAor). OAs are potent inhibitors of protein phosphatase 2A (PP2A) and are cytotoxic, necessitating expansion of the concept of OA equivalents to all relevant bioactivities. In this study, we determined OA equivalents for respective OA members in PP2A inhibition and cytotoxicity assays. To secure result credibility, we used certified OAs, reference materials, and PP2A produced using genetic engineering. The relative ratio of the OA equivalents determined by PP2A inhibition assays for OA, DTX1, and DTX2 were 1.0:1.6:0.3, while the ratio determined using the cytotoxicity assays indicated 1.0:1.5:0.5. OA equivalents showed a similar tendency in the PP2A inhibition and cytotoxicity assays, and matched better with oral toxicity data than intraperitoneal toxicity in mice. The PP2A inhibition assay, which measures the core activity of the OAs, suggested a higher OA equivalent for DTX1 than that currently used.
Walleye Pollock Gadus chalcogrammus, a Species with Continuous Range from the Norwegian Sea to Korea, Japan, and California: New Records from the Siberian Arctic
Authors: Orlov Alexei M. , Rybakov Maxim O. , Vedishcheva Elena V. , Volkov Alexander A. , Orlova Svetlana Yu.
Published: October 2021

The first records of walleye pollock Gadus chalcogrammus Pallas, 1814 in the seas of the Siberian Arctic (the Laptev Sea, the Kara Sea, the southeastern Barents Sea), are documented. Information about the external morphology (morphometric and meristic characters), photos of sagittal otoliths and fish, and data on the sequences of the CO1 mtDNA gene are presented. The results of a comparative analysis indicate that walleye pollock caught in the Siberian Arctic do not differ in principle from North Pacific and North Atlantic individuals. Previous conclusions about the conspecificity of the walleye and Norwegian pollock Theragra finnmarchica are confirmed. New captures of walleye pollock in the Siberian Arctic allow us to formulate a hypothesis about its continuous species’ range from the coasts of Norway in the North Atlantic to the coasts of Korea, Japan, and California in the North Pacific. The few records of walleye pollock in the North Atlantic originate from the North Pacific due to the transport of early pelagic juveniles to the Arctic by currents through the Bering Strait and further active westward migrations of individuals which have switched to the bentho-pelagic mode of life.
The Mycorrizal Status in Vineyards Affected by Esca
Authors: Landi Lucia , Foglia Renzo , Murolo Sergio , Romanazzi Gianfranco
Published: October 2021

In this work we analyzed the relationship among native arbuscular mycorrhizal fungi (AMF) and vine roots affected by esca, a serious grapevine trunk disease. The AMF symbiosis was analyzed on the roots of neighboring plants (symptomatic and asymptomatic to esca) in 14 sites of three vineyards in Marche region (central–eastern Italy). The AMF colonization intensity, identified by non-vital staining, showed higher value in all esca symptomatic plants (ranging from 24.6% to 61.3%) than neighboring asymptomatic plants (from 17.4% to 57.6%). The same trend of Glomeromycota phylum abundance was detected by analyzing fungal operational taxonomic units (OTUs) linked to the AMF community, obtained by amplicon high throughput analysis of ITS 1 region. Overall, the highest amount of OTUs was detected on roots from symptomatic plants (0.42%), compared to asymptomatic roots (0.29%). Specific primer pairs for native Rhizophagus irregularis and Funneliformis mosseae AMF species, were designed in 28S rRNA and large subunit (LSU) ribosomal RNA, respectively, and droplet digital PCR protocol for absolute quantification was set up. A higher number of DNA copies of both fungal species were detected more frequently in symptomatic than asymptomatic vines. Our study suggests a relationship between esca and native AMF in grapevine. These results underline the importance of native rhizosphere microbial communities for a better knowledge of grapevine esca disease.
Phytophthora heterospora sp. nov., a New Pseudoconidia-Producing Sister Species of P. palmivora
Authors: Scanu Bruno , Jung Thomas , Masigol Hossein , Linaldeddu Benedetto Teodoro , Jung Marília Horta , Brandano Andrea , Mostowfizadeh-Ghalamfarsa Reza , Janoušek Josef , Riolo Mario , Cacciola Santa Olga
Published: October 2021

Since 1999, an unusual Phytophthora species has repeatedly been found associated with stem lesions and root and collar rot on young olive trees in Southern Italy. In all cases, this species was obtained from recently established commercial plantations or from nursery plants. Morphologically, the Phytophthora isolates were characterized by the abundant production of caducous non-papillate conidia-like sporangia (pseudoconidia) and caducous papillate sporangia with a short pedicel, resembling P. palmivora var. heterocystica. Additional isolates with similar features were obtained from nursery plants of Ziziphus spina-christi in Iran, Juniperus oxycedrus and Capparis spinosa in Italy, and mature trees in commercial farms of Durio zibethinus in Vietnam. In this study, morphology, breeding system and growth characteristics of these Phytophthora isolates with peculiar features were examined, and combined mitochondrial and nuclear multigene phylogenetic analyses were performed. The proportion between pseudoconidia and sporangia varied amongst isolates and depended on the availability of free water. Oogonia with amphigynous antheridia and aplerotic oospores were produced in dual cultures with an A2 mating type strain of P. palmivora, indicating all isolates were A1 mating type. Phylogenetically, these isolates grouped in a distinct well-supported clade sister to P. palmivora; thus, they constitute a separate taxon. The new species, described here as Phytophthora heterospora sp. nov., proved to be highly pathogenic to both olive and durian plants in stem inoculation tests.
Bioinformatics and Transcriptome Analysis of CFEM Proteins in Fusarium graminearum
Authors: Chen Lingqiao , Wang Haoyu , Yang Junhua , Yang Xianli , Zhang Mengyuan , Zhao Zhihui , Fan Yingying , Wang Cheng , Wang Jianhua
Published: October 2021

Fusarium blight of wheat is usually caused by Fusarium graminearum, and the pathogenic fungi will secrete effectors into the host plant tissue to affect its normal physiological process, so as to make it pathogenic. The CFEM (Common in Fungal Extracellular Membrane) protein domain is unique to fungi, but it is not found in all fungi. The CFEM protein contained in F. graminearum may be closely related to pathogenicity. In this study, 23 FgCFEM proteins were identified from the F. graminearum genome. Then, features of these proteins, such as signal peptide, subcellular localization, and transmembrane domains, etc., were analyzed and candidate effectors were screened out. Sequence alignment results revealed that each FgCFEM protein contains one CFEM domain. The amino acids of the CFEM domain are highly conserved and contain eight spaced cysteines, with the exception that FgCFEM8, 9, and 15 lack two cysteines and three cysteines were missed in FgCFEM18 and FgCFEM22. A recently identified CFEM_DR motif was detected in 11 FgCFEMs, and importantly we identified two new conserved motifs containing about 29 and 18 amino acids (CFEM_WR and CFEM_KF), respectively, in some of FgCFEM proteins. Transcriptome analysis of the genes encoding CFEM proteins indicated that all the CFEM-containing genes were expressed during wheat infection, with seven and six genes significantly up- and down-regulated, respectively, compared with in planta and in vitro. Based on the above analysis, FgCFEM11 and FgCFEM23 were predicted to be F. graminearum effectors. This study provides the basis for future functional analyses of CFEM proteins in F. graminearum.
Influence of Genetic Polymorphisms on Clinical Outcomes of Glatiramer Acetate in Multiple Sclerosis Patients
Authors: Zarzuelo-Romero María José , Pérez-Ramírez Cristina , Cura Yasmín , Carrasco-Campos María Isabel , Marangoni-Iglecias Luciana María , Ramírez-Tortosa María Carmen , Jiménez-Morales Alberto
Published: October 2021

Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease of autoimmune origin, in which inflammation and demyelination lead to neurodegeneration and progressive disability. Treatment is aimed at slowing down the course of the disease and mitigating its symptoms. One of the first-line treatments used in patients with MS is glatiramer acetate (GA). However, in clinical practice, a response rate of between 30% and 55% is observed. This variability in the effectiveness of the medication may be influenced by genetic factors such as polymorphisms in the genes involved in the pathogenesis of MS. Therefore, this review assesses the impact of genetic variants on the response to GA therapy in patients diagnosed with MS. The results suggest that a relationship exists between the effectiveness of the treatment with GA and the presence of polymorphisms in the following genes: CD86, CLEC16A, CTSS, EOMES, MBP, FAS, TRBC1, IL1R1, IL12RB2, IL22RA2, PTPRT, PVT1, ALOX5AP, MAGI2, ZAK, RFPL3, UVRAG, SLC1A4, and HLA-DRB1*1501. Consequently, the identification of polymorphisms in these genes can be used in the future as a predictive marker of the response to GA treatment in patients diagnosed with MS. Nevertheless, there is a lack of evidence for this and more validation studies need to be conducted to apply this information to clinical practice.
A Comparative Analysis of the Piezoelectric Ultrasonic Appliance and Trephine Bur for Apical Location: An In Vitro Study
Authors: Cáceres Madroño Esther , Rodríguez Torres Paulina , Oussama Soraya , Zubizarreta-Macho Álvaro , Bufalá Pérez María , Mena-Álvarez Jesús , Riad Deglow Elena , Hernández Montero Sofía
Published: October 2021

To compare and contrast the accuracy of piezoelectric ultrasonic insert (PUI) and trephine bur (TB) osteotomy site preparation techniques for apical location. (1) Material and methods: A total of 138 osteotomy site preparations were randomly distributed into one of two study groups. Group A: TB technique (n = 69) and B: PUI technique (n = 69). A preoperative cone-beam computed tomography scan and an intraoral scan were performed and uploaded to implant-planning software to plan the virtual osteotomy site preparations for apical location. Subsequently, the osteotomy site preparations were performed in the experimental models with both osteotomy site preparation techniques and a postoperative CBCT scan was performed and uploaded into the implant-planning software and matched with the virtually planned osteotomy site preparations to measure the deviation angle and horizontal deviation as captured at the coronal entry point and apical end-point between osteotomy site preparations using Student’s t-test statistical analysis. (2) Results: The paired t-test found statistically significant differences at the coronal entry-point deviations (p = 0.0104) and apical end-point deviations (p = 0.0104) between the TB and PUI study groups; however, no statistically significant differences were found in the angular deviations (p = 0.309) between the trephine bur and piezoelectric ultrasonic insert study groups. (3) Conclusions: The results showed that the TB is more accurate than the PUI for apical location.
Association of Prenatal Maternal Anemia with Tics and Tourette’s Syndrome in Offspring
Authors: Liu Yi-Chun , Chen Vincent Chin-Hung , Liao Yin-To , Chen Yi-Lung
Published: October 2021

Iron deficiency anemia (IDA) accounts for most of the anemia in pregnancy, and iron is essential for neurodevelopment. Tics and Tourette’s syndrome (TS) are neurodevelopmental disorders that manifest in childhood. A few studies reported an inconclusive association between iron deficiency and tics in children. No study has investigated the relationship between prenatal maternal anemia and tics in children. We aimed to assess the relationship between prenatal anemia exposure and the incidence of tics or TS in offspring. We linked the Taiwan National Health Insurance Research Database to the Maternal and Child Health Database for the analysis and identified 153,854 children with prenatal anemia exposure and 2,014,619 children without prenatal anemia exposure from 2004 to 2016 and followed them through 2017. Cox regression models were applied to compare the risk of tics or TS between the exposed and nonexposed groups. Among the exposed group, 37,832 were exposed at ≤12 weeks of gestational age (GA) and 116,022 at >12 weeks of GA. We observed an increased risk of tics and TS in those exposed at ≤12 weeks compared with the nonexposed group (adjusted hazard ratio (aHR) = 1.23, 95% confidence interval (CI): 1.12–1.34). The result remained consistent after adjusting for birth year, sex, birth order, maternal age, low-income levels, gestational age, birth weight, and alcohol use and smoking during pregnancy (aHR = 1.16, CI: 1.04–1.28). Fetuses exposed to maternal anemia at ≤12 weeks of GA are at high risk of tics or TS. However, this effect was attenuated to insignificance in the sibling comparison. Our study highlights the importance of detection of anemia during pregnancy and proper timing of iron supplementation.
Diversity Is Not Everything
Authors: Scott Drew A. , Eckhoff Kathryn D. , Lorenz Nicola , Dick Richard , Swab Rebecca M.
Published: October 2021

Since the passage of legislation in 1977, Appalachian mineland reclamation is typically completed using non-native C3 grasses and forbs. Alternatively, reclamation with native prairie (C4) grasses and forbs offers a more ecologically friendly alternative that can contribute to native plant conservation and potentially improve soil properties more quickly than shallower rooted C3 cool-season grasses. We assessed the establishment of native prairie after reclamation, evaluating three treatments for six years after planting—traditional cool season planting, native prairie planted at light density, and native prairie planted at heavy density. All treatments reached the objectives of reclamation—percentage of ground covered by vegetation—within 2 years after planting. All treatments at all sites, except for one site by treatment combination near a forest, showed an increase in plant species richness and Shannon–Wiener diversity in the first four years of reclamation, a peak around 5 years, and subsequent decrease. Little difference in plant richness and Shannon–Wiener diversity among treatments was observed. However, the two native seed mixes quickly diverged from the traditional mix in terms of community structure and diverged further over time, with both native treatments heading towards a more desirable native prairie grassland state, while the traditional mix remained dominated by non-native cool season grasses. The native treatments also exhibited greater increase in microbial biomass and fungi:bacteria ratio over time compared to the traditional mix. Soil organic carbon increased over time regardless of seed mix treatment. Exchangeable base cations and phosphorus generally decreased over time, as expected, regardless of seed mix treatment, likely due to uptake from established plants. Native grassland species were able to establish despite inclusion of some traditional species in the native mix. Native plant establishment likely resulted in benefits including pollinator resources, bird and wildlife habitat, and increased soil health, and we recommend that native prairie mixes be used directly in reclamation moving forward, as they are able to meet reclamation goals while establishing a successful native prairie plant community.
How Do Network Embeddedness and Environmental Awareness Affect Farmers’ Participation in Improving Rural Human Settlements?
Authors: Xie Jinhua , Yang Gangqiao , Wang Ge , Xia Wei
Published: October 2021

Based on social embeddedness theory, this paper aims to explore the influence mechanism of network embeddedness and environmental awareness on farmers’ participation in improving rural human settlements (IRHS). This research applies the Logit model and the Bootstrap method, using survey data from 495 farmers in Hubei Province, China. The results show that: (1) relational embeddedness has a significant negative impact on the centralized treatment of farmers’ domestic sewage, implying that strengthening the relationship between farmers and households helps to provide them with centralized treatment for domestic sewage; (2) environmental awareness has a significant positive impact on the centralized treatment of farmers’ domestic sewage, implying that the enhancement of farmers’ environmental awareness increases the promotion centralized treatment for domestic sewage; and (3) structural embeddedness can further affects farmers’ environmental awareness and then affects their participation in the centralized treatment of domestic sewage, implying that environmental awareness has a mediating effect between structural embeddedness and the centralized treatment of farmers’ domestic sewage. Overall, it is necessary not only to encourage the establishment of extension and discussion networks for farmers (relational embeddedness) to participate in IRHS but also to improve environmental education for farmers, especially by increasing their access to environmental knowledge and information (environmental awareness in mountainous areas, and, finally to support farmers. The relationship between the members and the village cadres (structural embeddedness) can further improve farmers’ awareness of participation in IRHS to better guide them in the centralized treatment of domestic waste and domestic sewage.
Community Perceptions of Tree Risk and Management
Authors: Judice Abbie , Gordon Jason , Abrams Jesse , Irwin Kris
Published: October 2021

Urban forests (trees growing in urban and peri-urban areas, including villages and large cities) are vital to mitigating the effects of climate change and urbanization but require special considerations such as risk mitigation in developed landscapes. Despite abundant research on risk perceptions of natural hazards, there is limited knowledge about risk perceptions associated with urban trees. As such, this research examines community perceptions of urban tree risk mitigation with a focus on four cities in the U.S. south. To better understand risk perceptions and mitigation, this study employs key informant interviews with community members. Guided by a socio-ecological resilience framework, the findings identify factors affecting resident attitudes towards tree management on the individual parcel and the community levels. The findings benefit tree risk governance in the face of climate variability, which increases societal and environmental vulnerability in urban settings.
Research on the Spatial Differences and Convergence Mechanism of Urban Land Use Efficiency under the Background of Regional Integration: A Case Study of the Yangtze River Economic Zone, China
Authors: Ge Kun , Zou Shan , Chen Danling , Lu Xinhai , Ke Shangan
Published: October 2021

Revealing the spatial differences and convergence mechanism of urban land use efficiency (ULUE) under the background of regional integration is of great significance for exploring the coordinated promotion path of ULUE. We attempted to build a theoretical framework to interpret ULUE spatial convergence under the background of regional integration and build a ULUE “green” evaluation system under multi-objective constraints. Based on this, we employed the super efficiency slack-based model (SBM), exploratory spatial data analysis, and spatial convergence model incorporated into the spatial weight matrix to re-examine the true level, spatial differences, and convergence mechanism of ULUE in the Yangtze River Economic Zone from 2003 to 2019 on a city scale. The results show that: (1) during the investigation period, ULUE in the Yangtze River Economic Zone has obvious spatial disequilibrium and spatial correlation characteristics; (2) there are absolute β-space convergence and conditional β-space convergence of ULUE in the whole Yangtze River Economic Zone and its upstream, midstream, and downstream areas; (3) driven by government management, industrial development, and spatial error effects, the convergence time of ULUE in the whole Yangtze River Economic Zone and its upstream, midstream, and downstream areas is obviously shortened.
Underground Land Administration from 2D to 3D: Critical Challenges and Future Research Directions
Authors: Saeidian Bahram , Rajabifard Abbas , Atazadeh Behnam , Kalantari Mohsen
Published: October 2021

The development and use of underground space is a necessity for most cities in response to rapid urbanisation. Effective underground land administration is critical for sustainable urban development. From a land administration perspective, the ownership extent of underground assets is essential for planning and managing underground areas. In some jurisdictions, physical structures (e.g., walls, ceilings, and utilities) are also necessary to delineate the ownership extent of underground assets. The current practice of underground land administration focuses on the ownership of underground space and mostly relies on 2D survey plans. This inefficient and fragmented 2D-based underground data management and communication results in several issues including boundary disputes, underground strikes, delays and disruptions in projects, economic losses, and urban planning issues. This study provides a review of underground land administration from three common aspects: legal, institutional, and technical. A range of important challenges have been identified based on the current research and practice. To address these challenges, the authors of this study propose a new framework for 3D underground land administration. The proposed framework outlines the future research directions to upgrade underground land administration using integrated 3D digital approaches.
Impact of Artificial Elements on Mountain Landscape Perception: An Eye-Tracking Study
Authors: Guo Suling , Sun Wei , Chen Wen , Zhang Jianxin , Liu Peixue
Published: October 2021

The landscape is an essential resource for attracting tourists to a destination, but this resource has long been overused by tourism development. Tourists and scholars have begun noticing the interference of human structures in the natural environment and how this can change the meaning of a landscape. In this study, the impact of artificial elements on mountain landscapes was investigated by measuring the characteristics of visual perception and a landscape value assessment using eye-tracking analysis. Furthermore, this study includes socio-demographic features for testing whether they have an impact on landscape perception. The results show that human structures impact both visual perception and the perceived value of landscapes. Hotels and temples attract more visual attention than a purely natural landscape. Modern hotels appear to have a negative influence on mountain landscape valuation, while temples with unique culture have positive impacts. Socio-demographic groups differ significantly in how they observe landscape images and, to a degree, how they value the landscape therein. Our study should be of value to landscape planning and tourism policy making.
The Quest for Signals in Noise: Leveraging Experiential Variation to Identify Bilingual Phenotypes
Authors: Beatty-Martínez Anne L. , Titone Debra A.
Published: October 2021

Increasing evidence suggests that bilingualism does not, in itself, result in a particular pattern of response, revealing instead a complex and multidimensional construct that is shaped by evolutionary and ecological sources of variability. Despite growing recognition of the need for a richer characterization of bilingual speakers and of the different contexts of language use, we understand relatively little about the boundary conditions of putative “bilingualism” effects. Here, we review recent findings that demonstrate how variability in the language experiences of bilingual speakers, and also in the ability of bilingual speakers to adapt to the distinct demands of different interactional contexts, impact interactions between language use, language processing, and cognitive control processes generally. Given these findings, our position is that systematic variation in bilingual language experience gives rise to a variety of phenotypes that have different patterns of associations across language processing and cognitive outcomes. The goal of this paper is thus to illustrate how focusing on systematic variation through the identification of bilingual phenotypes can provide crucial insights into a variety of performance patterns, in a manner that has implications for previous and future research.
Cytoskeletal Tensegrity in Microgravity
Authors: Gardiner John
Published: October 2021

In order for Man to venture further into Space he will have to adapt to its conditions, including microgravity. Life as we know it has evolved on Earth with a substantial gravitational field. If they spend considerable time away from Earth, astronauts experience physiological, mental, and anatomical changes. It is not clear if these are pathological or adaptations. However, it is true that they experience difficulties on their return to stronger gravity. The cytoskeleton is a key site for the detection of gravitational force within the body, due to its tensegrity architecture. In order to understand what happens to living beings in space, we will need to unravel the role cytoskeletal tensegrity architecture plays in the building and function of cells, organs, the body, and mind.
Comprehensive Characterization of Androgen-Responsive circRNAs in Prostate Cancer
Authors: Kong Zhe , Lu Yali , Wan Xuechao , Luo Jun , Li Dujian , Huang Yan , Wang Chenji , Li Yao , Xu Yaoting
Published: October 2021

The androgen receptor (AR) signaling pathway plays an important role in the initiation and progression of prostate cancer. Circular RNAs (circRNAs), the novel noncoding RNAs without 5′ to 3′ polarity or 3′ poly (A), play an important role in multiple diseases. However, the potential roles of androgen-responsive circRNAs in prostate cancer remain unclear. In this study, we identified 3237 androgen-responsive circRNAs and 1954 androgen-responsive mRNAs after dihydrotestosterone (DHT) stimulation using microarray. Among them, the expression of 1296 androgen-responsive circRNAs was consistent with that of their parent genes, and we thought AR might regulate the expression of these circRNAs at the transcriptional level. In addition, 1941 circRNAs expression was not consistent with their parent genes, and we speculated that AR may regulate the expression of those circRNAs at the posttranscriptional level through affecting alternative splicing. Analyzing the androgen-responsive circRNAs regulated at the posttranscriptional level, we identified two key RNA binding proteins (RBPs), WTAP and TNRC6, using the circInteractome database, which may play important role in the biogenesis of androgen-responsive circRNAs. Furthermore, we explored the potential biological functions and predicted the molecular mechanisms of two dysregulated circRNAs (circNFIA and circZNF561) in prostate cancer. In this study, we revealed that circNFIA was upregulated in prostate cancer tissues and plasma samples from patients with prostate cancer; circNFIA may play an oncogenic role in prostate cancer. In contrast, circZNF561 was downregulated and may act as a tumor suppressor in prostate cancer. Our results suggest that androgen-responsive circRNAs might regulate the progression of prostate cancer and could be novel diagnostic biomarkers.
Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner
Authors: Cheng Yan , Kim Woong-Ki , Wellman Laurie L. , Sanford Larry D. , Guo Ming-Lei
Published: October 2021

In this study, we investigated autophagy, glial activation status, and corticotropin releasing factor (CRF) signaling in the brains of mice after 5 days of sleep fragmentation (SF). Three different brain regions including the striatum, hippocampus, and frontal cortex were selected for examination based on roles in sleep regulation and sensitivity to sleep disruption. For autophagy, we monitored the levels of various autophagic induction markers including beclin1, LC3II, and p62 as well as the levels of lysosomal associated membrane protein 1 and 2 (LAMP1/2) and the transcription factor EB (TFEB) which are critical for lysosome function and autophagy maturation stage. For the status of microglia and astrocytes, we determined the levels of Iba1 and GFAP in these brain regions. We also measured the levels of CRF and its cognate receptors 1 and 2 (CRFR1/2). Our results showed that 5 days of SF dysregulated autophagy in the striatum and hippocampus but not in the frontal cortex. Additionally, 5 days of SF activated microglia in the striatum but not in the hippocampus or frontal cortex. In the striatum, CRFR2 but not CRFR1 was significantly increased in SF-experienced mice. CRF did not alter its mRNA levels in any of the three brain regions assessed. Our findings revealed that autophagy processes are sensitive to short-term SF in a region-specific manner and suggest that autophagy dysregulation may be a primary initiator for brain changes and functional impairments in the context of sleep disturbances and disorders.
Biological Invasion of Fish Parasite Neoergasilus japonicus (Harada, 1930) (Copepoda: Ergasilidae) in Lake Grand Laoucien, France: A Field Study on Life Cycle Parameters and Reasons for Unusual High Population Density
Authors: Alekseev Victor , Cuoc Corinne , Jamet Dominique , Jamet Jean-Louis , Chappaz Remi
Published: October 2021

The fish parasite, Neoergasilus japonicus (Harada, 1930), native to Eastern Siberia and the Amur River catchment area, invaded European water bodies in the middle of the last century, possibly due to the human-mediated distribution of fish in the Amur complex (i.e., the genera Hypophthalmichthys and Ctenopharyngodon). In the deep karst lake, Grand Laoucien (Marseille area, France), this species had an unusually high population density (from 1000 ind./ m3 in zooplankton to 4000 ind./ m3 in the nearshore area) during the free-living period of its life cycle. The annual cycle of N. japonicus includes a 5-month overwintering of fertilized females attached to fish fins and, following this, a five- to six-generation chain from March to November, when the free-living stages in the population alternate with parasite females which attach to their hosts for breeding. The population density of the parasites in zooplankton increased exponentially from spring to autumn, which positively correlated with temperature. We found a strong correlation between N. japonicus density and the community development of microphytobenthos, but not between N. japonicus and phyto- or zooplankton dynamics. The local contributing factors included a seasonal three-fold decrease in water levels and the development of anoxia in profundal waters, which led to a high ambient fish density and thus susceptibility to the parasite. Although the free-living parasite represented only 1% of zooplankton production, it consumed up to 25% of small invertebrate productivity. The maximum intensity of infection reached 140 parasites per fish, or 4.14 per g of weight. The high infection of fish with this parasite, in our opinion, indicated the danger it poses to the local ichthyofauna, which first encountered this new parasite.
On Analyzing Capnogram as a Novel Method for Screening COVID-19: A Review on Assessment Methods for COVID-19
Authors: Malarvili M. B. , Alexie Mushikiwabeza , Dahari Nadhira , Kamarudin Anhar
Published: October 2021

In November 2019, the novel coronavirus disease COVID-19 was reported in Wuhan city, China, and was reported in other countries around the globe. COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Strategies such as contact tracing and a vaccination program have been imposed to keep COVID-19 under control. Furthermore, a fast, noninvasive and reliable testing device is needed urgently to detect COVID-19, so that contact can be isolated and ringfenced before the virus spreads. Although the reverse transcription polymerase chain reaction (RT-PCR) test is considered the gold standard method for the diagnosis of SARS-CoV-2 infection, this test presents some limitations which cause delays in detecting the disease. The antigen rapid test (ART) test, on the other hand, is faster and cheaper than PCR, but is less sensitive, and may limit SARS-CoV-2 detection. While other tests are being developed, accurate, noninvasive and easy-to-use testing tools are in high demand for the rapid and extensive diagnosis of the disease. Therefore, this paper reviews current diagnostic methods for COVID-19. Following this, we propose the use of expired carbon dioxide (CO2) as an early screening tool for SARS-CoV-2 infection. This system has already been developed and has been tested on asthmatic patients. It has been proven that expired CO2, also known as capnogram, can help differentiate between respiratory conditions and, therefore, could be used to detect SARS-CoV-2 infection, as it causes respiratory tract-related diseases.
Structure Tuning and Electrical Properties of Mixed PVDF and Nylon Nanofibers
Authors: Černohorský Petr , Pisarenko Tatiana , Papež Nikola , Sobola Dinara , Ţălu Ştefan , Částková Klára , Kaštyl Jaroslav , Macků Robert , Škarvada Pavel , Sedlák Petr
Published: October 2021

The paper specifies the electrostatic spinning process of specific polymeric materials, such as polyvinylidene fluoride (PVDF), polyamide-6 (PA6, Nylon-6) and their combination PVDF/PA6. By combining nanofibers from two different materials during the spinning process, new structures with different mechanical, chemical, and physical properties can be created. The materials and their combinations were subjected to several measurements: scanning electron microscopy (SEM) to capture topography; contact angle of the liquid wettability on the sample surface to observe hydrophobicity and hydrophilicity; crystallization events were determined by differential scanning calorimetry (DSC); X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and Fourier-transform infrared spectroscopy (FT-IR) to describe properties and their changes at the chemical level. Furthermore, for the electrical properties of the sample, the dielectric characteristics and the piezoelectric coefficient were measured. The advantage of the addition of co-polymers was to control the properties of PVDF samples and understand the reasons for the changed functionality. The innovation point of this work is the complex analysis of PVDF modification caused by mixing with nylon PA6. Here we emphasize that the application of nylon during the spin influences the properties and structure (polarization, crystallization) of PVDF.
Effect of Steel-Cutting Technology on Fatigue Strength of Steel Structures: Tests and Analyses
Authors: Rowiński Sławomir
Published: October 2021

This paper presents the results of comparative fatigue tests carried out on steel S355J2N specimens cut out using different cutting methods, i.e., plasma cutting, water jet cutting, and oxyacetylene cutting. All the specimens were subjected to cyclic loading from which appropriate S-N curves were obtained. Furthermore, face-of-cut hardness and roughness measurements were carried out to determine the effect of the cutting method on the fatigue strength of the tested steel. The fatigue strength results were compared with the standard S-N fatigue curves. The fatigue strength of the specimens cut out with oxyacetylene was found to be higher than that of the specimens cut out with plasma even though the surface roughness after cutting with plasma was smaller than in the case of the other cutting technology. This was due to the significant effect of material hardening in the heat-affected zones. The test results indicate that, in comparison with the effect of the cutting technology, the surface condition of the specimens has a relatively small effect on their fatigue strength.
Natural Cinnamic Acid Derivatives: A Comprehensive Study on Structural, Anti/Pro-Oxidant, and Environmental Impacts
Authors: Gryko Kamila , Kalinowska Monika , Ofman Piotr , Choińska Renata , Świderski Grzegorz , Świsłocka Renata , Lewandowski Włodzimierz
Published: October 2021

Cinnamic acid (CA), p-coumaric acid (4-hydroxycinnamic acid, 4-HCA), caffeic acid (3,4-vdihydroxycinnamic acid, 3,4-dHCA), and 3,4,5-trihydroxycinnamic acid (3,4,5-tHCA) were studied for their structural, anti-/pro-oxidant properties and biodegradability. The FT-IR, FT-Raman, UV/Vis, 1H and 13C NMR, and quantum chemical calculations in B3LYP/6-311++G** were performed to study the effect on number and position of hydroxyl group in the ring on the molecular structure of molecules. The antioxidant properties of the derivatives were examined using DPPH● and HO● radicals scavenging assays, ferric ion reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), inhibition of linoleic acid oxidation, as well as the biological antioxidant assay with Saccharomyces cerevisiae. Moreover, the pro-oxidant activity of compounds in Trolox oxidation assay was estimated. The effect of the derivatives on environment on the basis of increasing the carbon and nitrogen compounds transformation processes occurring in biological wastewater treatment was studied.
Production of Aluminum AA6061 Hybrid Nanocomposite from Waste Metal Using Hot Extrusion Process: Strength Performance and Prediction by RSM and Random Forest
Authors: Msebawi Muntadher Sabah , Leman Zulkiflle , Shamsudin Shazarel , Tahir Suraya Mohd , Aiza Jaafar Che Nor , Ariff Azmah Hanim Mohamed , Zahari Nur Ismarrubie , Abdellatif Abdallah
Published: October 2021

To date, various studies have analysed the effects of reinforced ceramic on the properties of AA6061 recycled aluminum alloy chips, such as the tensile strength and fractography. However, a comprehensive analysis of the properties of hybrid composite with the addition of nano-silica oxide and nano-copper oxide reinforcements is still very limited. Therefore, this study aimed to optimise the factors comprising the preheating temperature (PHT), preheating time (PHti), and volume fraction (VF) of reinforcements then determine their impacts on the physical and mechanical properties of the recycled solid-state extruded composite aluminum chips. A total of 45 specimens were fabricated through the hot extrusion technique. The response surface methodology (RSM) was employed to study the optimisation at a PHT range of 450–550 °C with PHti of 1–3 h and VF of 1–3 vol% for both reinforcements (SiO2 and CuO). Moreover, a random forest (RF) model was developed to optimize the model based on a metaheuristic method to improve the model performance. Based on the experimental results the RF model achieve better results than response surface methodology (RSM). The functional quadratic regression is curvature and the tested variable shows stable close data of the mean 0 and α2. Based on the Pareto analysis, the PHT and VF were key variables that significantly affected the UTS, microhardness, and density of the product. The maximum properties were achieved at an optimum PHT, PHti, and VF of 541 °C, 2.25 h, 1 vol% SiO2 and 2.13 vol% CuO, respectively. Furthermore, the morphological results of the tensile fractured surface revealed the homogenous distribution of nano-reinforced CuO and SiO2 particles in the specimens’ structure.
Physical Properties of Mineral Fibers Depending on the Mineralogical Composition
Authors: Bombac David , Lamut Martin , Mrvar Primož , Širok Brane , Bizjan Benjamin
Published: October 2021

A developed methodology for determining the physical properties of mineral fibers prepared from different input mixtures under the same spinning wheel conditions is described and discussed. Energy dispersive X-ray fluorescence spectroscopy was combined with simultaneous thermal analysis and thermogravimetry to study the mineralogical composition and typical melting and crystallization temperatures. The mechanical properties measured with nanoindentation were related to the mineralogical properties and the results obtained are in agreement with the literature. The developed methodology shows reliable performance and demonstrates the ability to study the mechanical properties of mineral fibers, their mineralogical composition, and thermal properties. The presented experimental methodology opens up the possibility of researching the mechanical properties of mineral fibers for the purpose of defining production recipes in the field of mineral thermal insulation materials.
Study on the Behavior of Electrochemical Extraction of Cobalt from Spent Lithium Cobalt Oxide Cathode Materials
Authors: Li Hui , Li Haotian , Li Chenxiao , Liang Jinglong , Yan Hongyan , Xu Zhengzhen
Published: October 2021

The molten salt electrochemical method was used to reduce the Co in spent LiCoO2. The reduction mechanism of Co (III) in LiCoO2 was analyzed by cyclic voltammetry, square wave voltammetry, and open circuit potential. The reduction process of Co (III) on Fe electrode was studied in NaCl-CaCl2-LiCoO2 molten salt system at 750 °C. The results show that the reduction process of Co (III) is a two-step reduction: Co (III) → Co (II) → Co (0) and they are all quasi-reversible processes controlled by diffusion. Phase analysis (XRD) shows that Li+ and Cl2− in the molten salt form LiCl electrolysis experiments with different voltages were carried out, which proved the stepwise reduction of Co in LiCoO2.
Fabrication of Highly Porous and Pure Zinc Oxide Films Using Modified DC Magnetron Sputtering and Post-Oxidation
Authors: Park Se-Yong , Rho Soon-Ho , Lee Hwan-Seok , Kim Kyoung-Min , Lee Hee-Chul
Published: October 2021

Porous films of metals and metal oxides exhibit larger surface areas and higher reactivities than those of dense films. Therefore, they have gained growing attention as potential materials for use in various applications. This study reports the use of a modified direct current magnetron sputtering method to form porous Zn-ZnO composite films, wherein a subsequent wet post-oxidation process is employed to fabricate pure porous ZnO films. The porous Zn-ZnO composite films were initially formed in clusters, and evaluation of their resulting properties allowed the optimal conditions to be determined. An oxygen ratio of 0.3% in the argon gas flow resulted in the best porosity, while a process pressure of 14 mTorr was optimal. Following deposition, porous ZnO films were obtained through rapid thermal annealing in the presence of water vapor, and the properties and porosities of the obtained films were analyzed. An oxidation temperature of 500 °C was optimal, with an oxidation time of 5 min giving a pure ZnO film with 26% porosity. Due to the fact that the films produced using this method are highly reliable, they could be employed in applications that require large specific surface areas, such as sensors, supercapacitors, and batteries.
Load-Bearing Capacity of Beams Reinforced with Composite Rebar in Regard to Existing Guidelines
Authors: Olczyk Norbert , Błyszko Jarosław , Techman Mateusz
Published: October 2021

Non-metallic reinforcement such as fiber-reinforced polymer (FRP) is now being increasingly used in construction. Despite numerous similarities, elements reinforced with non-metallic bars work differently from the ones reinforced with steel bars, including cracking and failure mode. The examination of the stress state in these elements, so important for their proper design, raises many difficulties. The article presents the results of tests of bended beams reinforced with GFRP bars. The results of the experimental tests were compared with calculations based on selected design instructions. The results have shown that beams reinforced with GFRP exhibit increased cracking, higher deflection, and often mode of failure through crushing of concrete. The results have shown that in bended elements reinforced with the GFRP bars, the rebar often does not achieve the strength declared by the manufacturer. The study has shown that theoretical values of load-bearing capacity of beams reinforced with composite rebar differ greatly between different guidelines and instruction. The analysis showed that the use of GFRP bars as a replacement for steel bars is possible in demanding environmental conditions. However, excessive deflections and cracks may result in limited application due to overall serviceability requirements of the element.