bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeted mutagenesis of the CYP79D1 gene via CRISPR/Cas9-mediated genome editing results in lower levels of cyanide in cassava
Authors: Juma Bicko Steve , Mukami Asunta , Mweu Cecilia , Ngugi Mathew Piero , Mbinda Wilton
Published: October 2022

Cassava is the world’s most essential food root crop, generating calories to millions of Sub-Saharan African subsistence farmers. Cassava leaves and roots contain toxic quantities of the cyanogenic glycoside linamarin. Consumption of residual cyanogens results in cyanide poisoning due to conversion of the cyanogens to cyanide in the body. There is a need for acyanogenic cassava cultivars in order for it to become a consistently safe and acceptable food, and commercial crop. In recent years, the CRISPR/Cas system, has proven to be the most effective and successful genome editing tool for gene function studies and crop improvement. In this study, we performed targeted mutagenesis of the MeCYP79D1 gene in exon 3, using CRISPR/Cas9, via Agrobacterium-mediated transformation. The vector design resulted in knockout in cotyledon-stage somatic embryos regenerated under hygromycin selection. Eight plants were recovered and genotyped. DNA sequencing analysis revealed that the tested putative transgenic plants carried mutations within the MeCYP79D1 locus, with deletions and substitutions being reported upstream and downstream of the PAM sequence, respectively. The levels of linamarin and evolved cyanide present in the leaves of mecyp79d1 lines were reduced up to seven-fold. Nevertheless, the cassava linamarin and cyanide were not completely eliminated by the MeCYP79D1 knockout. Our results indicate that CRISPR/Cas9-mediated mutagenesis is as an alternative approach for development of cassava plants with lowered cyanide content.
Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis
Authors: Lin Xiaoli , Xiao Yu , Song Yongping , Gan Cong , Deng Xingguang , Wang Peng , Liu Jialong , Jiang Zhishu , Peng Limei , Zhou Dahu , He Xiaopeng , Bian Jianmin , Zhu Changlan , Liu Bo , He Haohua , Xu Jie
Published: October 2022

In plants, MAP65 preferentially cross-links the anti-parallel microtubules (MTs) and plays an important role for cytokinesis. However, the functions of MAP65 isoforms in rice (Oryza sativa. L) are largely unknown. Here, we identified two MAP65-3 homologs in rice, OsMAP65-3.1 and OsMAP65-3.2. We found that both OsMAP65-3.1 and OsMAP65-3.2 were similar in dimerization and location to AtMAP65-3, and the expression of either rice genes driven by the AtMAP65-3 promoter suppressed the cytokinesis failure and growth defect of atmap65-3. However, OsMAP65-3.1 with native promoter also recovered the atmap65-3, but OsMAP65-3.2 with its own promoter had no effects. OsMAP65-3.1 but not OsMAP65-3.2 was actively expressed in tissues enriched with dividing cells. R1R2R3-Myb (MYB3R) transcription factors directly bound to the OsMAP65-3.1 promoter but not that of OsMAP65-3.2. Furthermore, osmap65-3.2 had no obvious phenotype, while either osmap65-3.1 or osmap65-3.1(+/-) was lethal. The eminent MTs around the daughter nuclei and cytokinesis defects were frequently observed in OsMAP65-3.1-defective plants. Taken together, our findings suggest that OsMAP65-3.1, rather than OsMAP65-3.2, plays essential roles in rice cytokinesis resulting from their differential expression which were passably directly regulated by OsMYB3Rs.
Deep structured populations of geographically isolated nipa (Nypa fruticans Wurmb.) in the Indo-West Pacific revealed using microsatellite markers
Authors: Mantiquilla Junaldo A. , Shiao Meng-Shin , Lu Hsueh-Yu , Sridith Kitichate , Sidique Siti Nordahliawate M. , Liyanage Wasantha Kumara , Chu Ya-Ling , Shih Huie-Chuan , Chiang Yu-Chung
Published: October 2022

Nipa (Nypa fruticans Wurmb.) is an important mangrove palm species, but it is understudied due to lack of information on genetic patterns within its distribution range. In this study, we identified 18 informative microsatellite markers to assess genetic variations among local populations in the Indo-West Pacific (IWP). Results showed population stratification based on high genetic differentiation (FST = 0.22131) with the Mantel test indicating significance to isolation-by-distance. We found a pronounced differentiation between the west populations in Sri Lanka and east populations in Southeast Asia. The east populations around the South China Sea were more genetically similar than those along the Malacca Strait and Java Sea. These genetic clines were shaped by ocean circulations and seasonal monsoon reversals as plausible factors. The Malacca Strait was confirmed as both a genetic and a geographic barrier rather than a corridor according to the Monmonier plot. Simulations of directional migration indicated a statistically strong contemporary genetic connectivity from west to east where Sri Lankan immigrants were detected as far as central Philippines via long-distance dispersal. This is the first report on the recent migration patterns of nipa using microsatellites. Assignment of first-generation (F0) immigrants suggested Mainland Southeast Asia as a melting pot due to the admixture associated with excess of homozygosity. The western populations were recent expansions that emerged in rapid succession based on a phylogram as supported by footprints of genetic drift based on bottleneck tests.
Molecular insights into mechanisms underlying thermo-tolerance in tomato
Authors: Singh Achuit K. , Mishra Pallavi , Kashyap Sarvesh Pratap , Karkute Suhas G. , Singh Prabhakar Mohan , Rai Nagendra , Bahadur Anant , Behera Tusar K.
Published: October 2022

Plant productivity is being seriously compromised by climate-change-induced temperature extremities. Agriculture and food safety are threatened due to global warming, and in many cases the negative impacts have already begun. Heat stress leads to significant losses in yield due to changes in growth pattern, plant phonologies, sensitivity to pests, flowering, grain filling, maturity period shrinkage, and senescence. Tomato is the second most important vegetable crop. It is very sensitive to heat stress and thus, yield losses in tomato due to heat stress could affect food and nutritional security. Tomato plants respond to heat stress with a variety of cellular, physiological, and molecular responses, beginning with the early heat sensing, followed by signal transduction, antioxidant defense, osmolyte synthesis and regulated gene expression. Recent findings suggest that specific plant organs are extremely sensitive to heat compared to the entire plant, redirecting the research more towards generative tissues. This is because, during sexual reproduction, developing pollens are the most sensitive to heat. Often, just a few degrees of temperature elevation during pollen development can have a negative effect on crop production. Furthermore, recent research has discovered certain genetic and epigenetic mechanisms playing key role in thermo-tolerance and have defined new directions for tomato heat stress response (HSR). Present challenges are to increase the understanding of molecular mechanisms underlying HS, and to identify superior genotypes with more tolerance to extreme temperatures. Several metabolites, genes, heat shock factors (HSFs) and microRNAs work together to regulate the plant HSR. The present review provides an insight into molecular mechanisms of heat tolerance and current knowledge of genetic and epigenetic control of heat-tolerance in tomato for sustainable agriculture in the future. The information will significantly contribute to improve breeding programs for development of heat tolerant cultivars.
Variation in the main health-promoting compounds and antioxidant activity of different organs of Wasabi (Eutrema japonicum) from two producing areas
Authors: Di Hongmei , Cui Cexian , Fang Pengcheng , Ma Junying , He Maolin , Li Mengyao , Lu Wei , Zhang Fen , Zheng Yangxia
Published: October 2022

Wasabi (Eutrema japonicum), also known as Japanese horseradish, is a perennial herb widely used in Japanese cuisine for its special flavour. The health-promoting phytochemicals and antioxidant capacity of four organs (leaf, petiole, rhizome, and root) of two cultivars (Chuankui–1 and Chuankui–2) of wasabi from two producing areas, Leibo and Guangyuan in Sichuan Province, China, were investigated in this study. The results showed that leaves were rich in pigments, soluble protein, ascorbic acid, and total phenolics and had the highest antioxidant capacity. Soluble sugars were highest in the petioles and were 1.1- to 5-fold higher than those in the other three organs. Glucosinolates and glucosinolate breakdown products (GBPs) were the most abundant in rhizomes, and their maximum values were 271.61 mmol kg-1 DW and 249.78 mmol kg-1 DW, respectively. The rhizomes of Chuankui–1 in Leibo and the leaves of Chuankui–1 in Guangyuan were superior in terms of glucosinolates and GBPs. These findings provide new insights that will aid the use of wasabi cultivars; they also have implications for the environmental characteristics needed to obtain better quality wasabi products. In the future, metabolome and transcriptome can be used to analyze the potential mechanism of differences among typical varieties, origins and parts.
Interactive effect of elevated CO2 and drought on physiological traits of Datura stramonium
Authors: Javaid Muhammad Mansoor , Florentine Singarayer , Mahmood Athar , Wasaya Allah , Javed Talha , Sattar Abdul , Sarwar Naeem , Kalaji Hazem M. , Ahmad Hafiz Bashir , Worbel Jacek , Ahmed Mohammed A. A. , Telesiński Arkadiusz , Mojski Jacek
Published: October 2022

Rising atmospheric CO2concentrations are known to influence the response of many plants under drought. This paper aimed to measure the leaf gas exchange, water use efficiency, carboxylation efficiency, and photosystem II (PS II) activity ofDatura stramoniumunder progressive drought conditions, along with ambient conditions of 400 ppm (aCO2) and elevated conditions of 700 ppm (eCO2). Plants ofD. stramoniumwere grown at 400 ppm and 700 ppm under 100 and 60% field capacity in a laboratory growth chamber. For 10 days at two-day intervals, photosynthesis rate, stomatal conductance, transpiration rate, intercellular CO2concentration, water use efficiency, intrinsic water use efficiency, instantaneous carboxylation efficiency, PSII activity, electron transport rate, and photochemical quenching were measured. While drought stress had generally negative effects on the aforementioned physiological traits ofD. stramonium, it was found that eCO2concentration mitigated the adverse effects of drought and most of the physiological parameters were sustained with increasing drought duration when compared to that with aCO2.D. stramonium, which was grown under drought conditions, was re-watered on day 8 and indicated a partial recovery in all the parameters except maximum fluorescence, with this recovery being higher with eCO2compared to aCO2. These results suggest that elevated CO2mitigates the adverse growth effects of drought, thereby enhancing the adaptive mechanism of this weed by improving its water use efficiency. It is concluded that this weed has the potential to take advantage of climate change by increasing its competitiveness with other plants in drought-prone areas, suggesting that it could expand into new localities.
Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromones
Authors: Juteršek Mojca , Petek Marko , Ramšak Živa , Moreno-Giménez Elena , Gianoglio Silvia , Mateos-Fernández Rubén , Orzáez Diego , Gruden Kristina , Baebler Špela
Published: October 2022

Plant biofactories are a promising platform for sustainable production of high-value compounds, among which are insect sex pheromones, a green alternative to conventional insecticides in agriculture. Recently, we have constructed transgenic Nicotiana benthamiana plants (“Sexy Plants”, SxP) that successfully produce a blend of moth (Lepidoptera) sex pheromone compounds (Z)-11-hexadecen-1-ol and (Z)-11-hexadecenyl acetate. However, efficient biosynthesis of sex pheromones resulted in growth and developmental penalty, diminishing the potential for commercial use of SxP in biomanufacturing. To gain insight into the underlying molecular responses, we analysed the whole-genome transcriptome and evaluated it in relation to growth and pheromone production in low- and high-producing transgenic plants of v1.0 and v1.2 SxP lines. In our study, high-producing SxPv1.2 plants accumulated the highest amounts of pheromones but still maintained better growth compared to v1.0 high producers. For an in-depth biological interpretation of the transcriptomic data, we have prepared a comprehensive functional N. benthamiana genome annotation as well as gene translations to Arabidopsis thaliana, enabling functional information transfer by using Arabidopsis knowledge networks. Differential gene expression analysis, contrasting pheromone producers to wild-type plants, revealed that while only a few genes were differentially regulated in low-producing plants, high-producing plants exhibited vast transcriptional reprogramming. They showed signs of stress-like response, manifested as downregulation of photosynthesis-related genes and significant differences in expression of hormonal signalling and secondary metabolism-related genes, the latter presumably leading to previously reported volatilome changes. Further network analyses confirmed stress-like response with activation of jasmonic acid and downregulation of gibberellic acid signalling, illuminating the possibility that the observed growth penalty was not solely a consequence of a higher metabolic burden imposed upon constitutive expression of a heterologous biosynthetic pathway, but rather the result of signalling pathway perturbation. Our work presents an example of comprehensive transcriptomic analyses of disadvantageous stress signalling in N. benthamiana biofactory that could be applied to other bioproduction systems.
Brassinosteroids induced drought resistance of contrasting drought-responsive genotypes of maize at physiological and transcriptomic levels
Authors: Gillani Syed Faheem Anjum , Zhuang Zelong , Rasheed Adnan , Haq Inzamam Ul , Abbasi Asim , Ahmed Shakil , Wang Yinxia , Khan Muhammad Tajammal , Sardar Rehana , Peng Yunling
Published: October 2022

The present study investigated the brassinosteroid-induced drought resistance of contrasting drought-responsive maize genotypes at physiological and transcriptomic levels. The brassinosteroid (BR) contents along with different morphology characteristics, viz., plant height (PH), shoot dry weight (SDW), root dry weight (RDW), number of leaves (NL), the specific mass of the fourth leaf, and antioxidant activities, were investigated in two maize lines that differed in their degree of drought tolerance. In response to either control, drought, or brassinosteroid treatments, the KEGG enrichment analysis showed that plant hormonal signal transduction and starch and sucrose metabolism were augmented in both lines. In contrast, the phenylpropanoid biosynthesis was augmented in lines H21L0R1 and 478. Our results demonstrate drought-responsive molecular mechanisms and provide valuable information regarding candidate gene resources for drought improvement in maize crop. The differences observed for BR content among the maize lines were correlated with their degree of drought tolerance, as the highly tolerant genotype showed higher BR content under drought stress.
Genotype, mycorrhizae, and herbivory interact to shape strawberry plant functional traits
Authors: Whyle Robert L. , Trowbridge Amy M. , Jamieson Mary A.
Published: October 2022

Arbuscular mycorrhizal fungi (AMF) and herbivores are ubiquitous biotic agents affecting plant fitness. While individual effects of pairwise interactions have been well-studied, less is known about how species interactions above and belowground interact to influence phenotypic plasticity in plant functional traits, especially phytochemicals. We hypothesized that mycorrhizae would mitigate negative herbivore effects by enhancing plant physiology and reproductive traits. Furthermore, we expected genotypic variation would influence functional trait responses to these biotic agents. To test these hypotheses, we conducted a manipulative field-based experiment with three strawberry (Fragaria x ananassa) genotypes to evaluate plant phenotypic plasticity in multiple functional traits. We used a fully-crossed factorial design in which plants from each genotype were exposed to mycorrhizal inoculation, herbivory, and the combined factors to examine effects on plant growth, reproduction, and floral volatile organic compounds (VOCs). Genotype and herbivory were key determinants of phenotypic variation, especially for plant physiology, biomass allocation, and floral volatiles. Mycorrhizal inoculation increased total leaf area, but only in plants that received no herbivory, and also enhanced flower and fruit numbers across genotypes and herbivory treatments. Total fruit biomass increased for one genotype, with up to 30-40% higher overall yield depending on herbivory. Herbivory altered floral volatile profiles and increased total terpenoid emissions. The effects of biotic treatments, however, were less important than the overall influence of genotype on floral volatile composition and emissions. This study demonstrates how genotypic variation affects plant phenotypic plasticity to herbivory and mycorrhizae, playing a key role in shaping physiological and phytochemical traits that directly and indirectly influence productivity.
Carotenoid-derived bioactive metabolites shape plant root architecture to adapt to the rhizospheric environments
Authors: Ke Danping , Guo Jinggong , Li Kun , Wang Yujie , Han Xiaomeng , Fu Weiwei , Miao Yuchen , Jia Kun-Peng
Published: October 2022

Roots are important plant organs for the uptake of water and nutrient elements. Plant root development is finely regulated by endogenous signals and environmental cues, which shapes the root system architecture to optimize the plant growth and adapt to the rhizospheric environments. Carotenoids are precursors of plant hormones strigolactones (SLs) and ABA, as well as multiple bioactive molecules. Numerous studies have demonstrated SLs and ABA as essential regulators of plant root growth and development. In addition, a lot carotenoid-derived bioactive metabolites are recently identified as plant root growth regulators, such as anchorene, β-cyclocitral, retinal and zaxinone. However, our knowledge on how these metabolites affect the root architecture to cope with various stressors and how they interact with each other during these processes is still quite limited. In the present review, we will briefly introduce the biosynthesis of carotenoid-derived root regulators and elaborate their biological functions on root development and architecture, focusing on their contribution to the rhizospheric environmental adaption of plants.
Wind dynamics and leaf motion: Approaching the design of high-tech devices for energy harvesting for operation on plant leaves
Authors: Meder Fabian , Naselli Giovanna Adele , Mazzolai Barbara
Published: October 2022

High-tech sensors, energy harvesters, and robots are increasingly being developed for operation on plant leaves. This introduces an extra load which the leaf must withstand, often under further dynamic forces like wind. Here, we took the example of mechanical energy harvesters that consist of flat artificial “leaves” fixed on the petioles of N. oleander, converting wind energy into electricity. We developed a combined experimental and computational approach to describe the static and dynamic mechanics of the natural and artificial leaves individually and join them together in the typical energy harvesting configuration. The model, in which the leaves are torsional springs with flexible petioles and rigid lamina deforming under the effect of gravity and wind, enables us to design the artificial device in terms of weight, flexibility, and dimensions based on the mechanical properties of the plant leaf. Moreover, it predicts the dynamic motions of the leaf–artificial leaf combination, causing the mechanical-to-electrical energy conversion at a given wind speed. The computational results were validated in dynamic experiments measuring the electrical output of the plant-hybrid energy harvester. Our approach enables us to design the artificial structure for damage-safe operation on leaves (avoiding overloading caused by the interaction between leaves and/or by the wind) and suggests how to improve the combined leaf oscillations affecting the energy harvesting performance. We furthermore discuss how the mathematical model could be extended in future works. In summary, this is a first approach to improve the adaptation of artificial devices to plants, advance their performance, and to counteract damage by mathematical modelling in the device design phase.
Value-driven career attitude and job performance: An intermediary role of organizational citizenship behavior
Authors: Iqbal Muhammad Babar , Li Jianxun , Yang Shuili , Sindhu Paras
Published: October 2022

Background

Value-driven career attitude (VDCA) is considered a dimension of a protean career attitude (PCA). Individuals with this attitude seek out personally meaningful experiences and set their own psychological career success standards. This study investigates the association between value-driven career attitude and job performance. It looks at how organizational citizenship behavior affects the relationship between value-driven career attitudes and job performance.

Methods

A self-reported questionnaire was used to collect data from 400 random employees of SMEs in Pakistan during the early pandemic. We chose Cochran’s formula to determine the appropriate sample size, and PLS-SEM was used to analyze the model. P-O fit and self-determination theory is the theoretical lenses used in this study. The underpinning theories to this study enable the researchers to establish a link between VDCA, OCB, and job performance.

Results

By analyzing a sample of 400 employees from active enterprises, we discover that VDCA contributes to an improvement in job performance. Furthermore, OCB plays an intervening effect in the relationship between VDCA and job performance. Thus, the study provided evidence for the underpinning models of P-O fit and self-determination theory.

Conclusion

This study adds to the body of knowledge by investigating the connections between VDCA, OCB, and job performance in SMEs. The existing literature sheds scant light on these linkages, leaving a gap that this study will address. The current study expands on other themes to provide an in-depth analysis of many under-explored PCA outcomes, which may open up new avenues for future researchers to broaden and strengthen PCA with other constructs.
The mediating role of organizational intelligence in the relationship between quantum leadership and innovative behavior
Authors: Bilgen Ayşe , Elçi Meral
Published: October 2022

The present study aims to examine the mediator effect of organizational intelligence between quantum leadership and innovative behavior of employees in health organizations. It is aimed to examine the mediator effect of organizational intelligence between quantum leadership and innovative behavior of employees in health organizations. The data of the study were collected from 626 healthcare professionals working in hospitals and health centers in Istanbul, Turkey, by survey method. After the analysis of normality, validity and reliability, the hypotheses of the research were tested on the LISREL program using the structural equal modelling. The findings have showed that the three hypotheses of the research were confirmed: quantum leadership and organizational intelligence affect the innovative behaviors of employees positively and significantly, and organizational intelligence has a mediator effect between quantum leadership and the innovative behavior of employees. It is imperative that healthcare organizations, which are likely to encounter chaos and crisis risks such as the Covid-19 pandemic, follow innovation in order to provide better quality services. Quantum leadership and organizational intelligence are necessities in terms of revealing innovative behavior for healthcare organizations operating in a dynamic and chaotic environment. Since the effects of quantum leadership have not been studied in health organizations adequately, this study makes a contribution to fill this gap. In addition, it is predicted that the findings of this research will be illuminating in terms of innovative behaviors and leadership styles for health organizations that have a vital importance in all societies and have recently experienced a fact like COVID-19.
Study on lifestyle habits affecting sleep disorders at the undergraduate education stage in Xuzhou City, China
Authors: Wu Qi , Yuan Lei , Guo Xiao-Han , Li Jia-An , Yin Dehui
Published: October 2022

Background

In China, undergraduate students face both academic and career selection pressures, sleep is an important physiological process for them. Investigate the physical exercise, sleep quality of undergraduate students in the education stage in Xuzhou City, and analyze the factors affecting their sleep quality, to promote the health education and psychological health of undergraduate students.

Materials and methods

The Physical Activity Rating Scale-3 (PARS-3), the Pittsburgh Sleep Quality Index (PSQI), and the demographic information questionnaire were used to survey a whole-group sample of four undergraduate colleges and universities (Xuzhou Institute of Engineering, Xuzhou Medical University, China University of Mining and Technology, Jiangsu Normal University) in Xuzhou by cluster sampling, the general characteristics including gender, grade, height, weight, domicile, race, economic income, etc., were collected, and the data were analyzed and processed using chi-square tests and multi-factor logistic regression.

Results

3,366 valid questionnaires were collected from four undergraduate colleges and universities, including 1,355 males and 2,011 females. The detection rate of exercise in Jiangsu Normal University was lower than that in other universities, and the detection rate of sleep disorders was higher than that in other universities. Xuzhou Medical University, the highest detection rate of large exercise, Xuzhou Institute of Technology, the lowest detection rate of sleep disorders. There were significant differences in the detection rate of large amount of exercise among college students of different genders, grades, body types, and majors (χ2 = 259.172, P < 0.001; χ2 = 34.473, P < 0.001; χ2 = 36.026, P < 0.001; χ2 = 57.908, P < 0.001). There were significant differences in the detection rate of sleep disorders among college students with different gender, grade, family economic status, daily cell phone use time, cell phone purposeless usage, and exercise level (χ2 = 5.806, P = 0.016; χ2 = 47.5, P < 0.001; χ2 = 28.949, P < 0.001; χ2 = 55.866, P < 0.001; χ2 = 147.101, P < 0.001; χ2 = 9.129, P = 0.010). Multivariate logistic regression analysis showed that grade, family economic status, cell phone use time, cell phone purposeless usage is the main influencing factors of sleep disorders in college students.

Conclusion

The sleep problems of undergraduates are serious, especially in Jiangsu Normal University. Scientific and appropriate exercise is an important measure to solve the sleep problems of undergraduates. Colleges and universities should actively carry out health education, college students living habits such as cell phone use should be guided training.
A moderated-mediation analysis of performance appraisal politics perception and counterproductive work behavior
Authors: Wang Hong-Yan , Chen Zhi-Xia
Published: October 2022

Politics has become a common element in the performance appraisal process, and as decision recipients in this process, those appraised tend to be more sensitive to performance appraisal politics. This paper examines the mechanisms by which performance appraisal politics perception (PAPP) affects counterproductive work behavior (CWB) from the perspective of those appraised. The mediating effect of perceived organizational justice (POJ) and the moderating effect of political skill (PS) are incorporated into a parsimonious moderated-mediation model. A quantitative research approach is employed with survey data from 460 employees of large and medium-sized enterprises in Hubei Province (China), and structural equation modeling (SEM) and bootstrap analysis are used to test the proposed hypothesized relationships. The findings demonstrate that PAPP has a positive impact on CWB, and POJ partly mediates the relationship between PAPP and CWB. The results also reveal that individual PS moderates the positive correlation between PAPP and CWB. The academic and practical implications of these findings, as well as limitations and suggestions for future research, are also discussed.
Virtual leadership in relation to employees' mental health, job satisfaction and perceptions of isolation: A scoping review
Authors: Efimov Ilona , Rohwer Elisabeth , Harth Volker , Mache Stefanie
Published: October 2022

Background

The significant increase of digital collaboration, driven by the current COVID-19 pandemic, is resulting in changes in working conditions and associated changes in the stress-strain perception of employees. Due to the evident leadership influence on employees' health and well-being in traditional work settings, there is a need to investigate leadership in virtual remote work contexts as well. The objective of this scoping review was to assess the extent and type of evidence concerning virtual leadership in relation to employees' mental health, job satisfaction and perceptions of isolation.

Method

A search was undertaken in five databases, PubMed, Cochrane Library, PsycINFO, PSYNDEX and Web of Science, as well as reference lists of included articles on 9th February 2021 and an update on 28th September 2021. The search strategy was limited to English, German and French language, peer reviewed journal articles published from January 2000 onwards. This scoping review was conducted in accordance with the JBI methodology for scoping reviews. The methodological quality of the included studies was assessed using the JBI critical appraisal tools. A narrative synthesis was conducted.

Results

Nineteen studies met the eligibility criteria for this review. Overarching review findings suggested a positive link between virtual leadership and well-being, job satisfaction, and a negative link to psychological strain, stress and perceptions of isolation of digitally collaborating employees.

Conclusions

By mapping the available evidence on virtual leadership in relation to health and work-related employee outcomes, the review identified many research gaps in terms of content and methodology. Due to limited data, causal relationships were not derived. Future research is needed to examine the complex cause-and-effect relationships of virtual leadership in more detail.
The effects of 8-week complex training on lower-limb strength and power of Chinese elite female modern pentathlon athletes
Authors: Qiao Zining , Guo Zhenxiang , Li Bin , Liu Meng , Miao Guozhen , Zhou Limingfei , Bao Dapeng , Zhou Junhong
Published: October 2022

Complex training (CT) is a combination training method that alternates between performing high-load resistance training (RT) and plyometric training within one single session. The study aimed to examine the effects of CT on lower-limb strength and power of elite female modern pentathlon athletes under the new modern pentathlon format and competition rules. Ten female participants (age: 23.55 ± 2.22 years, weight: 60.59 ± 3.87 kg, height: 169.44 ± 4.57 cm, and training experience: 6.90 ± 2.08 years) of the national modern pentathlon team completed 8 weeks of RT as followed by 8 weeks of CT, with 2 weeks of break. Then, the participants conducted 8 weeks of CT, which included RT combined with plyometric training (e.g., drop jump and continuous jump). All stages of training were designed by the linear strength training period theories, requiring participants to train twice for the first 4 weeks and three times for the second 4 weeks. The one-repetition maximum (1RM) of squat, isometric mid-thigh pull (IMTP), counter-movement jump (CMJ), squat jump (SJ), pre-stretch augmentation percentage (PSAP), and reaction strength index (RSI) were assessed before and after both RT and CT training. One-way repeated-measure ANOVA models revealed that the 1RM of squat was significantly improved (p < 0.001) after RT as compared to pre-RT. No significant improvement in IMTP (p = 0.055), CMJ (p = 0.194), SJ (p = 0.692), PSAP (p = 0.087), and RSI (p = 0.238) was not observed. After CT, 1RM of squat (p < 0.001), IMTP (p < 0.035), CMJ (p < 0.001), SJ (p < 0.008), RSI (p < 0.006) were significant improved as compared to pre-RT, post-RT and pre-CT, while significant improvements in PSAP were observed as compared to pre-RT (p = 0.003) and pre-CT (p = 0.027), but not to post-RT (p = 0.156). This pilot study showed the promise of CT following RT to improve lower-limb strength and power in elite female modern pentathlon athletes. The findings are worthwhile to be confirmed in future studies with larger sample size and randomized design.
The robotic mentalist – On the influences of robots’ mentalizing abilities and external manipulative intent on people’s credibility attributions
Authors: Finkel Marcel , Krämer Nicole C.
Published: October 2022

Robots are used in various social interactions that require them to be perceived as credible agents (e.g., as product recommenders in shopping malls). To be rated credible (i.e., competent, trustworthy, and caring) a robot’s mentalizing abilities have shown to be beneficial because they allow a robot to infer users’ inner states, thus serving as a prerequisite for understanding their beliefs and attitudes. However, social robots are often deployed by private and thus profit-oriented companies. In such cases where an organization’s implied manipulative intent is salient, the effect of robots’ mentalizing abilities might be reversed. The reason for this is that mentalizing abilities could pose a persuasive threat to users rather than a feature for better understanding, thereby decreasing credibility attributions. These assumptions were tested in a three (robot’s mentalizing abilities) by two (external manipulative intent) between-subjects, pre-registered, laboratory experiment during which participants interacted with a social robot that recommended experience vouchers as potential gifts for participants’ target persons. Contrary to our assumptions, inferential statistical results revealed no significant differences in explicit or indirect credibility attributions caused by the experimental manipulation. The external manipulative intent of an organization using the robot caused no differences in participants’ behavioral intentions or evaluations of it. Furthermore, only participants’ attribution of empathic understanding to the robot varied significantly between the three mentalizing conditions. Our results suggest that people focus more on the robot than on the organization using it, causing potential opportunities for such organizations to hide their economic interests from the users.
The nature and content of rumination for head and neck cancer survivors
Authors: Menger Fiona , Deane Jennifer , Patterson Joanne M. , Fisher Peter , O’Hara James , Sharp Linda
Published: October 2022

Introduction

Head and neck cancer (HNC) diagnosis and treatment can be a significant life trauma. Some HNC survivors experience post-traumatic growth (PTG), which has been linked with better health-related quality-of-life. Empirical research on PTG, and theoretical models, point to the importance of being able to purposely make sense of the traumatic experience. Intrusive rumination, by contrast, is linked to poorer outcomes. This study explored HNC survivors’ experiences of rumination.

Methods

Twenty HNC survivors between 9 months and 5 years post-diagnosis were recruited (11 male, 9 female, age range 46–83). They had a range of HNC sub-types and cancer treatments. Participants underwent a semi-structured interview about their cancer diagnosis and treatment. Reflexive thematic analysis identified themes and sub-themes around rumination.

Results

Four themes with linked subthemes on the content and process of rumination were identified. Theme 1 was rumination and worry related to diagnosis. Here, survivors discussed how the HNC diagnosis and plans for treatment had dominated their initial thoughts. Theme 2 was processing the trauma of HNC. This theme reflected rumination on the traumatic experience of diagnosis and treatment and how the participant was reacting to it. Theme 3 was considering the impact. This theme encompassed retrospective thinking (e.g., on treatment decisions made) and comparisons between the participant now versus the early days after diagnosis. Theme 4 was continued rumination. This theme included ongoing intrusive and distressing rumination about the trauma and impact of cancer. Those who expressed ongoing rumination revisited fears (e.g., concerns about their future) or returned to negative experiences (e.g., distressing exchanges with healthcare professionals or what they perceived as poor care).

Conclusion

This study uniquely describes the nature and content of rumination following HNC. Early intrusive rumination is common and may reflect perceptions of cancer as an existential threat. Over time, rumination can become more reflective and move towards deliberate meaning-making. Some HNC survivors may benefit from interventions to reduce barriers to this transition. The content of distressing and difficult to control rumination (commonly focused on ongoing fears or inability to resolve difficult experiences) helps to identify those who may benefit from more directed psychological support.
A plausible link between the time-on-task effect and the sequential task effect
Authors: Mangin Thomas , Audiffren Michel , Lorcery Alison , Mirabelli Francesco , Benraiss Abdelrhani , André Nathalie
Published: October 2022

Mental fatigue can be studied by using either the time-on-task protocol or the sequential task protocol. In the time-on-task protocol, participants perform a long and effortful task and a decrease in performance in this task is generally observed over time. In the sequential task protocol, a first effortful or control task is followed by a second effortful task. The performance in the second task is generally worse after the effortful task than after the control task. The principal aim of the present experiment is to examine the relationship between these two decrements in performance while concomitantly using a sequential task protocol and assessing the performance of the first effortful task as a function of time-on-task. We expect a positive correlation between these two decrements in performance. A total of 83 participants performed a 30-min fatiguing mental task (i.e., a modified Stroop task) or a control task followed by a time-to-exhaustion handgrip task. As expected, this protocol combining the time-on-task and sequential task protocols allowed us to observe (1) a decrease in performance over time during the Stroop task, (2) a worst performance in the handgrip task after the Stroop task by comparison to the control task, (3) a positive correlation between these two effects. The decrease in performance during the Stroop task also correlated with the subjective measures of boredom and fatigue, whereas the detrimental effect observed in the handgrip task did not. Our findings suggest that the two fatigue-related phenomena share a common mechanism but are not completely equivalent.
Preliminary evidence of links between ayahuasca use and the corpus callosum
Authors: Simonsson Otto , Bouso José Carlos , Kurth Florian , Araújo Dráulio B. , Gaser Christian , Riba Jordi , Luders Eileen
Published: October 2022

Background

Recent research suggests that ayahuasca and its alkaloid-containing ingredients may be helpful in the treatment and prevention of certain movement and neurodegenerative disorders. However, such research is still in its infancy and more studies in normative samples seem necessary to explore effects of ayahuasca on clinically relevant brain structures, such as the corpus callosum.

Aims

The purpose of the present study was to investigate links between ayahuasca use and callosal structure in a normative sample.

Methods

Using structural imaging data from 22 ayahuasca users and 22 matched controls we compared the thickness of the corpus callosum between both groups at 100 equidistant points across the entire midsagittal surface. In addition, we investigated point-wise correlations between callosal thickness and the number of past ayahuasca sessions.

Results

The corpus callosum was significantly thicker within the isthmus in the ayahuasca group than in the control group. There was also a significant positive correlation between callosal thickness and the number of past ayahuasca sessions within the rostral body, albeit none of these effects survived corrections for multiple comparisons. No region was significantly thicker in the control than in the ayahuasca group, and no callosal region was negatively linked to ayahuasca use, even at uncorrected significance thresholds.

Conclusion

This study provides preliminary evidence of links between ayahuasca use and the corpus callosum. However, future studies need to replicate these findings, preferably using larger sample sizes and ideally also utilizing longitudinal research designs, to draw any practical conclusion and offer implications for follow-up clinical research.
Category learning in a recurrent neural network with reinforcement learning
Authors: Zhang Ying , Pan Xiaochuan , Wang Yihong
Published: October 2022

It is known that humans and animals can learn and utilize category information quickly and efficiently to adapt to changing environments, and several brain areas are involved in learning and encoding category information. However, it is unclear that how the brain system learns and forms categorical representations from the view of neural circuits. In order to investigate this issue from the network level, we combine a recurrent neural network with reinforcement learning to construct a deep reinforcement learning model to demonstrate how the category is learned and represented in the network. The model consists of a policy network and a value network. The policy network is responsible for updating the policy to choose actions, while the value network is responsible for evaluating the action to predict rewards. The agent learns dynamically through the information interaction between the policy network and the value network. This model was trained to learn six stimulus-stimulus associative chains in a sequential paired-association task that was learned by the monkey. The simulated results demonstrated that our model was able to learn the stimulus-stimulus associative chains, and successfully reproduced the similar behavior of the monkey performing the same task. Two types of neurons were found in this model: one type primarily encoded identity information about individual stimuli; the other type mainly encoded category information of associated stimuli in one chain. The two types of activity-patterns were also observed in the primate prefrontal cortex after the monkey learned the same task. Furthermore, the ability of these two types of neurons to encode stimulus or category information was enhanced during this model was learning the task. Our results suggest that the neurons in the recurrent neural network have the ability to form categorical representations through deep reinforcement learning during learning stimulus-stimulus associations. It might provide a new approach for understanding neuronal mechanisms underlying how the prefrontal cortex learns and encodes category information.
Creating a college adjustment index score for student veterans with and without disabilities
Authors: Umucu Emre
Published: October 2022

Higher education is a critical public health tool to achieve economic success, upward mobility, and improved quality of life. Yet, certain groups of students, including student veterans with and without disabilities (SVDs), are at high risk for course failure and dropout, partially due to challenges related to college adjustment. The purpose of this study is to develop a new college adjustment index score for SVDs. We had a total of 4 different research studies to examine the psychometric properties of our college adjustment index score. After conducting a series of psychometric analyses, we selected a total of 18-items. This 18-item tool may help clinicians and researchers conceptualize college adjustment among students through the lens of integrative Tinto’s model and positive psychology approaches. Our psychometric analyses revealed that this index tool is brief, reliable, and valid tool to capture college adjustment in SVDs.
Equitable suicide prevention for youth impacted by the juvenile legal system
Authors: Meza Jocelyn I. , Snyder Sean , Shanholtz Caroline
Published: October 2022

Suicide is the second leading cause of death for adolescents in the United States. Despite the already alarmingly high rates of suicide attempts among adolescents, youth involved in the juvenile legal system (JLS) are up to three times more likely to have suicide attempts than their peers not impacted by the JLS. This public health crisis is also a matter of health equity, knowing that ethnoracially minoritized youth, mainly Black and Latinx youth, have disproportionate contact with the JLS. In order to disrupt the current elevated rates of suicide among Black and Latinx youth involved in the JLS, there needs to be more concerted efforts to improve assessment and suicide prevention efforts in the JLS. There are various potential touch points of care for suicide prevention and the Sequential Intercept Model (SIM), which outlines community-based responses to the involvement of people with mental and substance use disorders in the criminal justice system, can be used as a strategic planning tool to outline possible equitable interventions across these various touch points. Our purpose is to provide a comprehensive picture of gaps and equitable opportunities for suicide prevention across each intercept of the SIM. We provide recommendations of priorities to promote health equity in suicide prevention for ethnoracially minoritized youth impacted by the JLS.