BACTERIAL SYMBIONT BIOACTIVE COMPOUND OF SOFT CORAL Sinularia flexibilis AND S. polydactyla
Authors: Rozirwan . , Bengen Dietriech G. , Chaidir . , Zamani Neviaty P. , Effendi Hefni 
Published: October 2017

<p><em>Symbiont bacteria on soft coral can produce bioactive compounds that plays an important role in chemical ecology and as a marine natural product.</em> <em>The purpose of this study was to find and characterize the antibacterial activities of active compounds extracted</em> <em>from bacterial symbionts of soft coral <span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">flexibilis</span> and <span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">polydactyla</span>. The methods used in this study were culture and isolation of bacterial symbionts, extraction of compounds, antibacterial bioassay, and identification of bioactive compounds using the LC-MS analyses. Four isolates of bacterial symbionts were obtained from two samples of soft corals, 2 isolates of <span style="text-decoration: underline;">Pseudomonas</span> <span style="text-decoration: underline;">diminuta</span> (A1) and <span style="text-decoration: underline;">Edwardsiellla</span> <span style="text-decoration: underline;">hoshinae</span> (A2) from soft coral <span style="text-decoration: underline;">S.</span> <span style="text-decoration: underline;">flexibilis</span>, and 2 isolates of <span style="text-decoration: underline;">E.</span> <span style="text-decoration: underline;">hoshinae</span> (B1) and <span style="text-decoration: underline;">P</span>. <span style="text-decoration: underline;">acidovorans</span> (B4) from <span style="text-decoration: underline;">S.</span> <span style="text-decoration: underline;">polydactila</span>. Antibacterial activity were found only from the extracts of bacterial symbionts <span style="text-decoration: underline;">P.</span> <span style="text-decoration: underline;">diminuta</span> (A1) and from  <span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">flexiblis</span> about 10.16 ± 0.3mm (for <span style="text-decoration: underline;">B</span>. <span style="text-decoration: underline;">subtilis</span>), 8.66 ± 0.8 mm (<span style="text-decoration: underline;">E.</span> <span style="text-decoration: underline;">coli</span>) and 9.86 ± 1.7mm (<span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">dysentri</span>). No antibacterial activity found from the extracts of <span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">polydactyla</span>. The results of LC MS analysis showed that the group of diterpenes sinularin produced by soft corals <span style="text-decoration: underline;">S</span>. <span style="text-decoration: underline;">flexibilis </span>and bacterial symbionts isolates of <span style="text-decoration: underline;">P</span>. <span style="text-decoration: underline;">diminuta</span> (A1).</em></p><p><em><strong><em>Keywords:  </em></strong><em>Bacterial Symbiont, Bioactive Compound, Antibacterial Activity, Soft Coral, Sinularia flexibilis, Sinularia polydactyla</em></em></p>