bluuebunny/crossref_metadata_2025 huggingface dataset:
Pedagogical Proposal of Tele-Exercise Based on “Square Stepping Exercise” in Preschoolers: Study Protocol
Authors: Domínguez-Muñoz Alberto , Carlos-Vivas Jorge , Barrios-Fernandez Sabina , Adsuar José Carmelo , Morenas-Martín Jesús , Garcia-Gordillo Miguel Angel , Domínguez-Muñoz Francisco Javier
Published: August 2021

Early childhood education aims to achieve the motor, cognitive, emotional, and social development of preschoolers by providing them with a variety of learning opportunities. The square-stepping exercise (SSE) is a balance and lower limb strength training programme used to prevent falls and stimulate cognitive function in older adults. This project aims to propose an SSE tele-exercise (Tele-SSE) protocol to evaluate its effects on the motor and cognitive development of children aged between 3 and 6 years. A randomized controlled trial with experimental (Tele-SSE) and control (general education) groups will be carried out. The application of Tele-SSE will be performed for 9 months (three times per week) and one additional follow-up after the intervention at the beginning of the next academic year. One-hundred and two preschoolers will be recruited and randomly distributed into the two groups: experimental (n = 51) and control (n = 51). Although the main outcome will be balance due to the nature of the SSE, outcomes will include physical and motor (body mass index, waist circumference, handgrip and lower-limb strength, speed-agility, and cardiorespiratory fitness) and cognitive (executive functions and attention, episodic memory, and language assessment, using the Fitness Assessment in the Preschool Battery (PREFIT) and The National Institutes of Health Toolbox—Early Childhood Cognition Battery. This project aims to improve cognitive and motor skills in preschoolers aged between 3 and 6 years old, based on a 9-month Tele-SSE intervention. If this intervention proves to be effective, it could be implemented in those centres, entities and associations specializing in early childhood education.
Social Belonging as the Main Concern for Achieving Life Satisfaction When Adapting to Parkinson’s Disease
Authors: Rosengren Lina , Forsberg Anna , Brogårdh Christina , Lexell Jan
Published: August 2021

Parkinson’s disease (PD) is a complex, progressive neurological condition that impacts daily life and reduces life satisfaction (LS). To achieve and maintain high LS, persons with PD (PwPD) must go through a process of change to adapt to their new life situation. However, our knowledge about this process is very limited. The aim of this study was to investigate the process of change, and the main concern in this process, in PwPD. To study the transitional experience of PwPD, an inductive qualitative approach, using Grounded Theory (GT), was employed. Thirteen participants (9 women, 3 men and 1 non-binary), with a mean age of 54 years (range from 47–62 years), participated in in-depth interviews. Data showed that social belonging is the main concern in the process of change for PwPD. In this process of change, they use strategies to comprehend, accept, adapt, and balance in their strive for social belonging, which in turn can enhance LS. Health care professionals can use this model with an interdisciplinary approach to support PwPD through a successful process of change to achieve social belonging, and thereby achieving and maintaining LS.
Changes in the Composition of the Soil Bacterial Community in Heavy Metal-Contaminated Farmland
Authors: Tseng Shu-chun , Liang Chih-ming , Chia Taipau , Ton Shan-shin
Published: August 2021

The structural changes of microorganisms in soil are the focus of soil indicators research. The purpose of this study was to investigate the changes in the composition of the soil bacterial community in heavy metal-contaminated soil. A total of six soil samples (two sampling times) were collected from contaminated farmland at three different depths (surface, middle, and deep layer). The pH value was measured. The concentrations of heavy metals (Cr, Ni, Cu, Zn, Cd, and Pb) and the soil bacterial community were analyzed using ICP-OES and 16S rRNA gene sequencing. The results of the two samplings showed that the pH value in the deep layer decreased from 6.88 to 6.23, and the concentrations of Cu, Zn, Cd, and Pb, with a smaller ion radius, increased by 16–28%, and Shannon, Chao1 increased by ~13%. The bacteria community composition at the three depths changed, but Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla. In the copper and zinc tolerance test, the isolated bacterium that was able to tolerate copper and zinc was Bacillus sp. We found that, the longer the heavy metal pollution was of concern, the higher the tolerance. These results can be used as references for the microbial remediation of heavy metal-contaminated farmland.
The Link between Individual Personality Traits and Criminality: A Systematic Review
Authors: Tharshini N. K. , Ibrahim Fauziah , Kamaluddin Mohammad Rahim , Rathakrishnan Balan , Che Mohd Nasir Norruzeyati
Published: August 2021

In addition to social and environmental factors, individual personality traits have intricately linked with maladaptive behaviour. Thus, the purpose of this article was to review the link between individual personality traits and criminality. A systematic review was conducted to obtain information regarding the link between individual personality traits with criminal behaviour in the Sage, Web of Science, APA PsycNet, Wiley Online Library, and PubMed databases. The results indicate that individual personality traits that contribute towards criminality are (i) psychopathy; (ii) low self-control; and (iii) difficult temperament. As an overall impact, the review is expected to provide in-depth understanding of the link between individual personality traits and criminality; hence, greater consideration will be given to the dimension of personality as a notable risk factor of criminal behaviour.
Development of a City-Scale Approach for Façade Color Measurement with Building Functional Classification Using Deep Learning and Street View Images
Authors: Zhang Jiaxin , Fukuda Tomohiro , Yabuki Nobuyoshi
Published: August 2021

Precise measuring of urban façade color is necessary for urban color planning. The existing manual methods of measuring building façade color are limited by time and labor costs and hardly carried out on a city scale. These methods also make it challenging to identify the role of the building function in controlling and guiding urban color planning. This paper explores a city-scale approach to façade color measurement with building functional classification using state-of-the-art deep learning techniques and street view images. Firstly, we used semantic segmentation to extract building façades and conducted the color calibration of the photos for pre-processing the collected street view images. Then, we proposed a color chart-based façade color measurement method and a multi-label deep learning-based building classification method. Next, the field survey data were used as the ground truth to verify the accuracy of the façade color measurement and building function classification. Finally, we applied our approach to generate façade color distribution maps with the building classification for three metropolises in China, and the results proved the transferability and effectiveness of the scheme. The proposed approach can provide city managers with an overall perception of urban façade color and building function across city-scale areas in a cost-efficient way, contributing to data-driven decision making for urban analytics and planning.
Exploration of the Cytoplasmic Function of Abnormally Fertilized Embryos via Novel Pronuclear-Stage Cytoplasmic Transfer
Authors: Fujimine-Sato Ayako , Kuno Takashi , Higashi Keiko , Sugawara Atsushi , Hiraga Hiroaki , Takahashi Aiko , Tanaka Keiko , Yokoyama Emi , Shiga Naomi , Watanabe Zen , Yaegashi Nobuo , Tachibana Masahito
Published: August 2021

In regular IVF, a portion of oocytes exhibit abnormal numbers of pronuclei (PN) that is considered as abnormal fertilization, and they are routinely discarded. However, it is known that abnormal ploidy still does not completely abandon embryo development and implantation. To explore the potential of cytoplasm from those abnormally fertilized oocytes, we developed a novel technique for the transfer of large cytoplasm between pronuclear-stage mouse embryos, and assessed its impact. A large volume of cytoplast could be efficiently transferred in the PN stage using a novel two-step method of pronuclear-stage cytoplasmic transfer (PNCT). PNCT revealed the difference in the cytoplasmic function among abnormally fertilized embryos where the cytoplasm of 3PN was developmentally more competent than 1PN, and the supplementing of fresh 3PN cytoplasm restored the impaired developmental potential of postovulatory “aged” oocytes. PNCT-derived embryos harbored significantly higher mitochondrial DNA copies, ATP content, oxygen consumption rate, and total cells. The difference in cytoplasmic function between 3PN and 1PN mouse oocytes probably attributed to the proper activation via sperm and may impact subsequent epigenetic events. These results imply that PNCT may serve as a potential alternative treatment to whole egg donation for patients with age-related recurrent IVF failure.
Peptide Stapling Improves the Sustainability of a Peptide-Based Chimeric Molecule That Induces Targeted Protein Degradation
Authors: Yokoo Hidetomo , Ohoka Nobumichi , Takyo Mami , Ito Takahito , Tsuchiya Keisuke , Kurohara Takashi , Fukuhara Kiyoshi , Inoue Takao , Naito Mikihiko , Demizu Yosuke
Published: August 2021

Peptide-based target protein degradation inducers called PROTACs/SNIPERs have low cell penetrability and poor intracellular stability as drawbacks. These shortcomings can be overcome by easily modifying these peptides by conjugation with cell penetrating peptides and side-chain stapling. In this study, we succeeded in developing the stapled peptide stPERML-R7, which is based on the estrogen receptor alpha (ERα)-binding peptide PERML and composed of natural amino acids. stPERML-R7, which includes a hepta-arginine motif and a hydrocarbon stapling moiety, showed increased α-helicity and similar binding affinity toward ERα when compared with those of the parent peptide PERML. Furthermore, we used stPERML-R7 to develop a peptide-based degrader LCL-stPERML-R7 targeting ERα by conjugating stPERML-R7 with a small molecule LCL161 (LCL) that recruits the E3 ligase IAPs to induce proteasomal degradation via ubiquitylation. The chimeric peptide LCL-stPERML-R7 induced sustained degradation of ERα and potently inhibited ERα-mediated transcription more effectively than the unstapled chimera LCL-PERML-R7. These results suggest that a stapled structure is effective in maintaining the intracellular activity of peptide-based degraders.
Effects of Sodium-Glucose Co-Transporter 2 Inhibitors on Vascular Cell Function and Arterial Remodeling
Authors: Durante William , Behnammanesh Ghazaleh , Peyton Kelly J.
Published: August 2021

Cardiovascular disease is the leading cause of morbidity and mortality in diabetes. Recent clinical studies indicate that sodium-glucose co-transporter 2 (SGLT2) inhibitors improve cardiovascular outcomes in patients with diabetes. The mechanism underlying the beneficial effect of SGLT2 inhibitors is not completely clear but may involve direct actions on vascular cells. SGLT2 inhibitors increase the bioavailability of endothelium-derived nitric oxide and thereby restore endothelium-dependent vasodilation in diabetes. In addition, SGLT2 inhibitors favorably regulate the proliferation, migration, differentiation, survival, and senescence of endothelial cells (ECs). Moreover, they exert potent antioxidant and anti-inflammatory effects in ECs. SGLT2 inhibitors also inhibit the contraction of vascular smooth muscle cells and block the proliferation and migration of these cells. Furthermore, studies demonstrate that SGLT2 inhibitors prevent postangioplasty restenosis, maladaptive remodeling of the vasculature in pulmonary arterial hypertension, the formation of abdominal aortic aneurysms, and the acceleration of arterial stiffness in diabetes. However, the role of SGLT2 in mediating the vascular actions of these drugs remains to be established as important off-target effects of SGLT2 inhibitors have been identified. Future studies distinguishing drug- versus class-specific effects may optimize the selection of specific SGLT2 inhibitors in patients with distinct cardiovascular pathologies.
Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus
Authors: Soldevila Ferran , Edwards Jane C. , Graham Simon P. , Crooke Helen R. , Werling Dirk , Steinbach Falko
Published: August 2021

Classical swine fever (CSF) is a highly contagious disease caused by the classical swine fever virus (CSFV). The live attenuated C-strain vaccine is highly efficacious, initiating protection within several days of delivery. The vaccine strain is detected in the tonsil early after inoculation, yet little is known of the role that tonsillar immune cells might play in initiating protection. Comparing the C-strain vaccine with the pathogenic CSFV Alfort-187 strain, changes in the myeloid cell compartment of the tonsil were observed. CSFV infection led to the emergence of an additional CD163+CD14+ cell population, which showed the highest levels of Alfort-187 and C-strain infection. There was also an increase in both the frequency and activation status (as shown by increased MHC-II expression) of the tonsillar conventional dendritic cells 1 (cDC1) in pigs inoculated with the C-strain. Notably, the activation of cDC1 cells coincided in time with the induction of a local CSFV-specific IFN-γ+ CD8 T cell response in C-strain vaccinated pigs, but not in pigs that received Alfort-187. Moreover, the frequency of CSFV-specific IFN-γ+ CD8 T cells was inversely correlated to the viral load in the tonsils of individual animals. Accordingly, we hypothesise that the activation of cDC1 is key in initiating local CSFV-specific CD8 T cell responses which curtail early virus replication and dissemination.
Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice
Authors: Neumann Silke , Campbell Katrin , Woodall Matthew J. , Evans Meghan , Clarkson Andrew N. , Young Sarah L.
Published: August 2021

Obesity is a major risk factor for developing cancer, with obesity-induced immune changes and inflammation in breast (BC) and colorectal cancer (CRC) providing a potential link between the two. This study investigates systemic effects of obesity on adaptive and innate immune cells in healthy and tumour-bearing mice. Immune cells from lean and obese mice were phenotyped prior to implantation of either BC (C57mg and EO771.LMB) or CRC (MC38) cells as tumour models. Tumour growth rate, tumour-infiltrating lymphocytes (TIL) and peripheral blood immune cell populations were compared between obese and lean mice. In vitro studies showed that naïve obese mice had higher levels of myeloid cells in the bone marrow and bone marrow-derived dendritic cells expressed lower levels of activation markers compared to cells from their lean counterparts. In the tumour setting, BC tumours grew faster in obese mice than in lean mice and lower numbers of TILs as well as higher frequency of exhausted T cells were observed. Data from peripheral blood showed lower levels of myeloid cells in tumour-bearing obese mice. This study highlights that systemic changes to the immune system are relevant for tumour burden and provides a potential mechanism behind the effects of obesity on cancer development and progression in patients.
Mechanisms Underlying the Protective Effect of the Peroxiredoxin-6 Are Mediated via the Protection of Astrocytes during Ischemia/Reoxygenation
Authors: Turovsky Egor A. , Varlamova Elena G. , Plotnikov Egor Y.
Published: August 2021

Ischemia-like conditions reflect almost the entire spectrum of events that occur during cerebral ischemia, including the induction of oxidative stress, Ca2+ overload, glutamate excitotoxicity, and activation of necrosis and apoptosis in brain cells. Mechanisms for the protective effects of the antioxidant enzyme peroxiredoxin-6 (Prx-6) on hippocampal cells during oxygen-glucose deprivation/reoxygenation (OGD/R) were investigated. Using the methods of fluorescence microscopy, inhibitory analysis, vitality tests and PCR, it was shown that 24-h incubation of mixed hippocampal cell cultures with Prx-6 does not affect the generation of a reversible phase of a OGD-induced rise in Ca2+ ions in cytosol ([Ca2+]i), but inhibits a global increase in [Ca2+]i in astrocytes completely and in neurons by 70%. In addition, after 40 min of OGD, cell necrosis is suppressed, especially in the astrocyte population. This effect is associated with the complex action of Prx-6 on neuroglial networks. As an antioxidant, Prx-6 has a more pronounced and astrocyte-directed effect, compared to the exogenous antioxidant vitamin E (Vit E). Prx-6 inhibits ROS production in mitochondria by increasing the antioxidant capacity of cells and altering the expression of genes encoding redox status proteins. Due to the close bond between [Ca2+]i and intracellular ROS, this effect of Prx-6 is one of its protective mechanisms. Moreover, Prx-6 effectively suppresses not only necrosis, but also apoptosis during OGD and reoxygenation. Incubation with Prx-6 leads to activation of the basic expression of genes encoding protective kinases—PI3K, CaMKII, PKC, anti-apoptotic proteins—Stat3 and Bcl-2, while inhibiting the expression of signaling kinases and factors involved in apoptosis activation—Ikk, Src, NF-κb, Caspase-3, p53, Fas, etc. This effect on the basic expression of the genome leads to the cell preconditions, which is expressed in the inhibition of caspase-3 during OGD/reoxygenation. A significant effect of Prx-6 is directed on suppression of the level of pro-inflammatory cytokine IL-1β and factor TNFα, as well as genes encoding NMDA- and kainate receptor subunits, which was established for the first time for this antioxidant enzyme. The protective effect of Prx-6 is due to its antioxidant properties, since mutant Prx-6 (mutPrx-6, Prx6-C47S) leads to polar opposite effects, contributing to oxidative stress, activation of apoptosis and cell death through receptor action on TLR4.
Adsorption on Ligand-Tethered Nanoparticles
Authors: Borówko Małgorzata , Staszewski Tomasz
Published: August 2021

We use coarse-grained molecular dynamics simulations to study adsorption on ligand-tethered particles. Nanoparticles with attached flexible and stiff ligands are considered. We discuss how the excess adsorption isotherm, the thickness of the polymer corona, and its morphology depend on the number of ligands, their length, the size of the core, and the interaction parameters. We investigate the adsorption-induced structural transitions of polymer coatings. The behavior of systems involving curved and flat “brushes” is compared.
JI017 Attenuates Oxaliplatin-Induced Cold Allodynia via Spinal TRPV1 and Astrocytes Inhibition in Mice
Authors: Lee Ji Hwan , Ji Hyunseung , Ko Seong-Gyu , Kim Woojin
Published: August 2021

Oxaliplatin, a well-known chemotherapeutic agent, can induce severe neuropathic pain, which can seriously decrease the quality of life of patients. JI017 is an herb mixture composed of Aconitum carmichaelii, Angelica gigas, and Zingiber officinale. Its anti-tumor effect has been reported; however, the efficacy of JI017 against oxaliplatin-induced allodynia has never been explored. Single oxaliplatin injection [6 mg/kg, intraperitoneal, (i.p.)] induced both cold and mechanical allodynia, and oral administration of JI017 (500 mg/kg) alleviated cold but not mechanical allodynia in mice. Real-time polymerase chain reaction (PCR) analysis demonstrated that the upregulation of mRNA of spinal transient receptor potential vanilloid 1 (TRPV1) and astrocytes following oxaliplatin injection was downregulated after JI017 treatment. Moreover, TRPV1 expression and the activation of astrocytes were intensely increased in the superficial area of the spinal dorsal horn after oxaliplatin treatment, whereas JI017 suppressed both. The administration of TRPV1 antagonist [capsazepine, intrathecal (i.t.), 10 μg] attenuated the activation of astrocytes in the dorsal horn, demonstrating that the functions of spinal TRPV1 and astrocytes are closely related in oxaliplatin-induced neuropathic pain. Altogether, these results suggest that JI017 may be a potent candidate for the management of oxaliplatin-induced neuropathy as it decreases pain, spinal TRPV1, and astrocyte activation.
A New Glycosyltransferase Enzyme from Family 91, UGT91P3, Is Responsible for the Final Glucosylation Step of Crocins in Saffron (Crocus sativus L.)
Authors: López-jimenez Alberto José , Frusciante Sarah , Niza Enrique , Ahrazem Oussama , Rubio-Moraga Ángela , Diretto Gianfranco , Gómez-Gómez Lourdes
Published: August 2021

Crocetin is an apocarotenoid formed from the oxidative cleavage of zeaxanthin, by the carotenoid cleavage enzymes CCD2 (in Crocus species) and specific CCD4 enzymes in Buddleja davidii and Gardenia jasminoides. Crocetin accumulates in the stigma of saffron in the form of glucosides and crocins, which contain one to five glucose molecules. Crocetin glycosylation was hypothesized to involve at least two enzymes from superfamily 1 UDP-sugar dependent glycosyltransferases. One of them, UGT74AD1, produces crocins with one and two glucose molecules, which are substrates for a second UGT, which could belong to the UGT79, 91, or 94 families. An in silico search of Crocus transcriptomes revealed six candidate UGT genes from family 91. The transcript profiles of one of them, UGT91P3, matched the metabolite profile of crocin accumulation, and were co-expressed with UGT74AD1. In addition, both UGTs interact in a two-hybrid assay. Recombinant UGT91P3 produced mostly crocins with four and five glucose molecules in vitro, and in a combined transient expression assay with CCD2 and UGT74AD1 enzymes in Nicotiana benthamiana. These results suggest a role of UGT91P3 in the biosynthesis of highly glucosylated crocins in saffron, and that it represents the last missing gene in crocins biosynthesis.
Endocytic Protein Defects in the Neural Crest Cell Lineage and Its Pathway Are Associated with Congenital Heart Defects
Authors: Arrigo Angelo B. , Lin Jiuann-Huey Ivy
Published: August 2021

Endocytic trafficking is an under-appreciated pathway in cardiac development. Several genes related to endocytic trafficking have been uncovered in a mutagenic ENU screen, in which mutations led to congenital heart defects (CHDs). In this article, we review the relationship between these genes (including LRP1 and LRP2) and cardiac neural crest cells (CNCCs) during cardiac development. Mice with an ENU-induced Lrp1 mutation exhibit a spectrum of CHDs. Conditional deletion using a floxed Lrp1 allele with different Cre drivers showed that targeting neural crest cells with Wnt1-Cre expression replicated the full cardiac phenotypes of the ENU-induced Lrp1 mutation. In addition, LRP1 function in CNCCs is required for normal OFT lengthening and survival/expansion of the cushion mesenchyme, with other cell lineages along the NCC migratory path playing an additional role. Mice with an ENU-induced and targeted Lrp2 mutation demonstrated the cardiac phenotype of common arterial trunk (CAT). Although there is no impact on CNCCs in Lrp2 mutants, the loss of LRP2 results in the depletion of sonic hedgehog (SHH)-dependent cells in the second heart field. SHH is known to be crucial for CNCC survival and proliferation, which suggests LRP2 has a non-autonomous role in CNCCs. In this article, other endocytic trafficking proteins that are associated with CHDs that may play roles in the NCC pathway during development, such as AP1B1, AP2B1, FUZ, MYH10, and HECTD1, are reviewed.
A New Perspective on Fluorapatite Dissolution in Hydrochloric Acid: Thermodynamic Calculations and Experimental Study
Authors: Tõnsuaadu Kaia , Kallas Juha , Kuusik Rein , Hacialioglu-Erlenheim Gizem , Trikkel Andres
Published: August 2021

Apatite (Ap) dissolution in diluted acids is well described in the literature, but in technological processes which use more concentrated acids, the reaction is fast, and it is complicated to follow the process kinetics. The relationship between pH change and the apatite dissolution rate depending on HCl concentration was studied by thermodynamic calculations and experiments with synthetic fluorapatite (FAp). On the basis of experimental pH measurements, the kinetics of dissolution was analyzed. The solution composition (P, Ca, F) was determined by wet chemical methods and the solid part was characterized by XRD and FTIR. It was shown that the amount of HCl needed for FAp dissolution depends on acid concentration. FAp dissolution rate cannot be deduced from solubility data of P, Ca or F as the secondary reactions of CaF2 and CaHPO4 formation take place simultaneously. It was found that the Ap dissolution rate can be followed by pH change.
Blinded by the Light: Artificial Light Lowers Mate Attraction Success in Female Glow-Worms (Lampyris noctiluca L.)
Authors: Van den Broeck Mira , De Cock Raphaël , Van Dongen Stefan , Matthysen Erik
Published: August 2021

Nocturnal light pollution from anthropogenic origin is increasing worldwide and is recognised as a major threat for nocturnal biodiversity. We studied the impact of artificial light on the mate attraction success of female common glow-worms (Lampyris noctiluca L.) by daily monitoring their glowing status in the field, acting as a proxy for mating status throughout the mating season. We found that females in dark surroundings typically stopped glowing after one night, indicating that they had mated, while females in illuminated areas glowed for significantly more nights, in some cases up to 15 nights. Our study confirms previous findings and hypotheses that females exposed to artificial light suffer from a reduced mate attraction success with a negative impact on populations.
Health, Wellbeing, and Prognosis of Australian Adolescents with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Case-Controlled Follow-Up Study
Authors: Josev Elisha K. , Cole Rebecca C. , Scheinberg Adam , Rowe Katherine , Lubitz Lionel , Knight Sarah J.
Published: August 2021

Background: The purpose of this study was to follow-up an Australian cohort of adolescents newly-diagnosed with ME/CFS at a tertiary paediatric ME/CFS clinic and healthy controls over a mean period of two years (range 1–5 years) from diagnosis. Objectives were to (a) examine changes over time in health and psychological wellbeing, (b) track ME/CFS symptomatology and fulfillment of paediatric ME/CFS diagnostic criteria over time, and (c) determine baseline predictors of ME/CFS criteria fulfilment at follow-up. Methods: 34 participants aged 13–18 years (25 ME/CFS, 23 controls) completed standardised questionnaires at diagnosis (baseline) and follow-up assessing fatigue, sleep quality and hygiene, pain, anxiety, depression, and health-related quality of life. ME/CFS symptomatology and diagnostic criteria fulfilment was also recorded. Results: ME/CFS patients showed significant improvement in most health and psychological wellbeing domains over time, compared with controls who remained relatively stable. However, fatigue, pain, and health-related quality of life remained significantly poorer amongst ME/CFS patients compared with controls at follow-up. Sixty-five percent of ME/CFS patients at baseline continued to fulfil ME/CFS diagnostic criteria at follow-up, with pain the most frequently experienced symptom. Eighty-two percent of patients at follow-up self-reported that they still had ME/CFS, with 79% of these patients fulfilling criteria. No significant baseline predictors of ME/CFS criteria fulfilment at follow-up were observed, although pain experienced at baseline was significantly associated with criteria fulfilment at follow-up (R = 0.6, p = 0.02). Conclusions: The majority of Australian adolescents with ME/CFS continue to fulfil diagnostic criteria at follow-up, with fatigue, pain, and health-related quality of life representing domains particularly relevant to perpetuation of ME/CFS symptoms in the early years following diagnosis. This has direct clinical impact for treating clinicians in providing a more realistic prognosis and highlighting the need for intervention with young people with ME/CFS at the initial diagnosis and start of treatment.
Comparative Impact of PD-1 and PD-L1 Inhibitors on Advanced Esophageal or Gastric/Gastroesophageal Junction Cancer Treatment: A Systematic Review and Meta-Analysis
Authors: Oh SuA , Kim Eunyoung , Lee Heeyoung
Published: August 2021

Programmed death 1 (PD-1) and PD ligand 1 (PD-L1) inhibitors have demonstrated varying effectiveness in treating esophageal or gastric/gastroesophageal junction (G/GEJ) cancer. Hence, this systematic review and meta-analysis evaluated the efficacy and safety of anti-PD-1/PD-L1 treatment in patients with esophageal or G/GEJ cancer by analyzing the types of medications. Randomized controlled trials comparing anti-PD-1/PD-L1 to control therapy were identified by searching PubMed, EMBASE, and ClinicalTrials.gov. The outcomes included overall survival (OS), progression-free survival (PFS) rates, and serious adverse events (SAEs), evaluating the differences in therapy types, including a comparison between PD-1 and PD-L1 inhibitors. Eight studies were included in the analysis. PD-1/PD-L1 inhibitors affected the overall OS rate increment without influencing the PFS rate (HR, 0.837; 95% CI, 0.753–0.929; p = 0.001; HR 0.991; 95% CI, 0.778–1.263; p = 0.942, respectively). Anti-PD-1 was significantly more beneficial for increasing OS and PFS than PD-L1 inhibitors. Anti-PD-1 and PD-L1 use was not significantly associated with SAE development in esophageal or G/GEJ cancer patients. PD-1/PD-L1 inhibitor use was associated with improved OS and PFS rate increase among PD-1 and PD-L1 inhibitors. Considering response variations to anti-PD-1/PD-L1 usage, more individualized treatments should be introduced in clinical practice.
Arterial Stiffness in Type 1 Diabetes: The Case for the Arterial Wall Itself as a Target Organ
Authors: González-Clemente José-Miguel , Cano Albert , Albert Lara , Giménez-Palop Olga , Romero Ana , Berlanga Eugenio , Vendrell Joan , Llauradó Gemma
Published: August 2021

Arterial stiffness (AS) integrates the cumulative burden of known and unknown cardiovascular risk factors on the elastic wall of large arteries along the lifespan of an individual. As a marker of vascular aging, AS is an independent predictor of cardiovascular events and improves cardiovascular risk prediction when added to the Framingham Risk Score. In addition, AS may affect the microvasculature and promote the development of microvascular complications. Its impact on both the macro- and microvasculature has led to the concept that the arterial wall itself should be considered as a target organ. Here, we review the biological and clinical consequences of AS on the macro- and microvasculature and the measurement of AS in routine clinical practice. We also discuss the pathophysiological mechanisms underpinning AS development using diabetes and, in particular, type 1 diabetes, as a disease model with a high risk of cardiovascular events and microvascular complications that are accelerated by AS.
Severe Myocardial Dysfunction after Non-Ischemic Cardiac Arrest: Effectiveness of Percutaneous Assist Devices
Authors: Manzo-Silberman Stéphane , Nix Christoph , Goetzenich Andreas , Demondion Pierre , Kang Chantal , Bonneau Michel , Cohen-Solal Alain , Leprince Pascal , Lebreton Guillaume
Published: August 2021

Introduction: Despite the improvements in standardized cardiopulmonary resuscitation, survival remains low, mainly due to initial myocardial dysfunction and hemodynamic instability. Our goal was to compare the efficacy of two left ventricular assist devices on resuscitation and hemodynamic supply in a porcine model of ventricular fibrillation (VF) cardiac arrest. Methods: Seventeen anaesthetized pigs had 12 min of untreated VF followed by 6 min of chest compression and boluses of epinephrine. Next, a first defibrillation was attempted and pigs were randomized to any of the three groups: control (n = 5), implantation of an percutaneous left ventricular assist device (Impella, n = 5) or extracorporeal membrane oxygenation (ECMO, n = 7). Hemodynamic and myocardial functions were evaluated invasively at baseline, at return of spontaneous circulation (ROSC), after 10–30–60–120–240 min post-resuscitation. The primary endpoint was the rate of ROSC. Results: Only one of 5 pigs in the control group, 5 of 5 pigs in the Impella group, and 5 of 7 pigs in the ECMO group had ROSC (p < 0.05). Left ventricular ejection fraction at 240 min post-resuscitation was 37.5 ± 6.2% in the ECMO group vs. 23 ± 3% in the Impella group (p = 0.06). No significant difference in hemodynamic parameters was observed between the two ventricular assist devices. Conclusion: Early mechanical circulatory support appeared to improve resuscitation rates in a shockable rhythm model of cardiac arrest. This approach appears promising and should be further evaluated.
The Review of the Autotomy of Agamid Lizards with Considerations about the Types of Autotomy and Regeneration
Authors: Ananjeva Natalia Borisovna , Gordeev Dmitry Anatolyevich , Korost Dmitry Vyacheslavovich
Published: August 2021

We present a review of the data on the intervertebral autotomy and regeneration of agamid lizards based on an analysis of information obtained over a 35-year period after the publication of thorough reviews (Arnold, 1984, 1988 and Bellairs, Bryant, 1985). It is supplemented by our own studies of 869 specimens of agamid lizards (Sauria, Agamidae) stored in the herpetological collections of the Zoological Institute of the Russian Academy of Sciences (St. Petersburg, Russia) and the Zoological Museum of the Moscow State University (Moscow, Russia), represented by 31 species of 16 genera. The manifestations of the ability for autotomy and regeneration in phylogenetic lineages within the family—Leiolepidinae, Amphibolurinae, Agaminae, Draconinae—are considered. A comparative morphological analysis of the structure of the caudal vertebrae was carried out using the Computer Microtomography Methods (micro-CT) in the following ecomorphological types of agama: (1) with developed abilities to caudal autotomy and regeneration, (2) with the ability to caudal autotomy but without regeneration and (3) without the ability to autotomy. The phenomenon of intervertebral autotomy (urotomy) in snakes is considered too. Possible ways of evolution of the ability to caudal autotomy as a defense strategy against predators are discussed in the phylogenetic context.
The Impact of COVID-19 on the Work of Property Valuers: A Glance at the Polish State of Play
Authors: Uhruska Małgorzata , Małkowska Agnieszka
Published: August 2021

This article presents how the COVID-19 pandemic affected the valuation profession in Poland in the early stages of its most severe restrictions and limitations. This study is the first to investigate the impact of COVID-19 on the professional activities of property valuers. In particular, it aims to identify the difficulties associated with valuers’ activities during the first lockdown and the impact of restrictions on business performance. The data analyzed come from a survey of Polish valuers in September 2020. The questions were of a closed-ended nature. Using a five-point Likert scale, respondents expressed their opinions on the difficulties and benefits of their work in the first COVID-19 period. The results show that the respondents experienced difficulties related to the pandemic and noted its negative impact on business performance. The most significant problem was the limited access to public institutions supporting the valuation process and providing market data on real estate transactions. The respondents also indicated other problems related to property valuation, as well as some positive effects for their business.
Social Networks: A Source of Lexical Innovation and Creativity in Contemporary Peninsular Spanish
Authors: Rodríguez Arrizabalaga Beatriz
Published: August 2021

There is no doubt that the Internet, where English is ubiquitous, has revolutionized our way of life. Socially, it has opened frontiers to such an extent that nowadays human beings can be permanently connected, no matter the distance between them, in virtual encounters where social networks play a crucial role. Linguistically, on the other hand, it has created a new global language which combines properties of written and oral speech. The Internet’s lexical level, in particular, is described as extremely innovative, creative and playful since it is full of neologisms, many of which are Anglicisms, coined to name the new realities constantly brought along with the evolution of the digital world. In order to demonstrate that social networks are indeed the source of a wide array of creative and playful neological Anglicisms in Peninsular Spanish, we have carried out a corpus-based analysis of the names of five current social and interactive forms of Internet communication in two contemporary Spanish corpora; specifically, that of the indirect social network generically known as the blog and those of the direct social networks Facebook, Instagram, Twitter and WhatsApp. Our study focuses, specifically, on four facets of these neological Anglicisms: (i) their date of introduction into the language; (ii) their frequency of occurrence; (iii) their diverse spellings; and (iv) finally, the different word-formation processes they enter.
Blockchain Solutions for International Logistics Networks along the New Silk Road between Europe and Asia
Authors: Aritua Bernard , Wagener Clemens , Wagener Norbert , Adamczak Michał
Published: August 2021

The primary research that underpins this paper seeks to explore the applications of blockchain technology on a specific international corridor and to draw policy implications for decision makers. To analyze the bottlenecks of operating on the New Silk Road and to identify opportunities for applying the blockchain technology on this corridor, a survey was conducted among main train operators and experts working on this route. These responses provide insight into the issues related to the adoption of blockchain technology from front-line actors. The top three challenges are lack of capacities, congestion at transshipment terminals, and slow border crossing. Through the application of blockchain technology, the operators are presented with opportunities for improved accuracy in the processing of data and information, higher reliability of information flows through failure-free transfer of information, and improved traceability of supply chains through irrevocable input of status information. Currently, 50% of the respondents have started to implement blockchain applications or have an actual interest to apply blockchain solutions. For a wider implementation of blockchain solutions, business models need to be developed allowing private and permissioned access that is accepted and open for parties involved. Policy makers should facilitate these digital innovations through flexible and harmonized legal regulations on an international level.
Aluminate-Based Nanostructured Luminescent Materials: Design of Processing and Functional Properties
Authors: Rojas-Hernandez Rocío Estefanía , Rubio-Marcos Fernando , Fernandez José Francisco , Hussainova Irina
Published: August 2021

Interest in luminescent materials has been continuously growing for several decades, looking for the development of new systems with optimized optical properties. Nowadays, research has been focused on the development of materials that satisfy specific market requirements in optoelectronics, radioelectronics, aerospace, bio-sensing, pigment applications, etc. Despite the fact that several efforts have made in the synthesis of organic luminescent materials, their poor stability under light exposure limits their use. Hence, luminescent materials based on inorganic phosphors are considered a mature topic. Within this subject, glass, glass-ceramics and ceramics have had great technological relevance, depending on the final applications. Supposing that luminescent materials are able to withstand high temperatures, have a high strength and, simultaneously, possess high stability, ceramics may be considered promising candidates to demonstrate required performance. In an ongoing effort to find a suitable synthesis method for their processing, some routes to develop nanostructured luminescent materials are addressed in this review paper. Several ceramic families that show luminescence have been intensively studied in the last few decades. Here, we demonstrate the synthesis of particles based on aluminate using the methods of sol-gel or molten salts and the production of thin films using screen printing assisted by a molten salt flux. The goal of this review is to identify potential methods to tailor the micro-nanostructure and to tune both the emission and excitation properties, focusing on emerging strategies that can be easily transferred to an industrial scale. Major challenges, opportunities, and directions of future research are specified.
Mechanical Properties of Porous Structures for Dental Implants: Experimental Study and Computational Homogenization
Authors: Jíra Aleš , Šejnoha Michal , Krejčí Tomáš , Vorel Jan , Řehounek Luboš , Marseglia Guido
Published: August 2021

A combined experimental and numerical study on titanium porous microstructures intended to interface the bone tissue and the solid homogeneous part of a modern dental implant is presented. A specific class of trabecular geometries is compared to a gyroid structure. Limitations associated with the application of the adopted selective laser melting technology to small microstructures with a pore size of 500 μm are first examined experimentally. The measured effective elastic properties of trabecular structures made of Ti6Al4V material support the computational framework based on homogenization with the difference between the measured and predicted Young’s moduli of the Dode Thick structure being less than 5%. In this regard, the extended finite element method is promoted, particularly in light of the complex sheet gyroid studied next. While for plastic material-based structures a close match between experiments and simulations was observed, an order of magnitude difference was encountered for titanium specimens. This calls for further study and we expect to reconcile this inconsistency with the help of computational microtomography.
Formamidinium Lead Iodide Perovskite Films with Polyvinylpyrrolidone Additive for Active Layer in Perovskite Solar Cells, Enhanced Stability and Electrical Conductivity
Authors: Kojić Vedran , Bohač Mario , Bafti Arijeta , Pavić Luka , Salamon Krešimir , Čižmar Tihana , Gracin Davor , Juraić Krunoslav , Leskovac Mirela , Capan Ivana , Gajović Andreja
Published: August 2021

In this paper, we studied the influence of polyvinylpyrrolidone (PVP) as a stabilization additive on optical and electrical properties of perovskite formamidinium lead iodide (FAPI) polycrystalline thin films on ZnO nanorods (ZNR). FAPI (as an active layer) was deposited from a single solution on ZNR (low temperature processed electron transport layer) using a one-step method with the inclusion of an anti-solvent. The role of PVP in the formation of the active layer was investigated by scanning electron microscopy and contact angle measurements to observe the effect on morphology, while X-ray diffraction was used as a method to study the stability of the film in an ambient environment. The effect of the PVP additive on the optical and electrical properties of the perovskite thin films was studied via photoluminescence, UV-Vis measurements, and electrical impedance spectroscopy. We have demonstrated that PVP inclusion in solution-processed perovskite FAPI thin films prevents the degradation of the film in an ambient atmosphere after aging for 2 months. The inclusion of the PVP also improves the infiltration of FAPI perovskite into ZnO nanostructures, increases electrical conductivity and radiative recombination of the photo-generated charge carriers. These results show promising information for promoting PVP stabilized FAPI perovskites for the new generation of photovoltaic devices.
Synergistical Performance Modification of Epoxy Resin by Nanofillers and Carboxyl-Terminated Liquid Nitrile–Butadiene Rubber
Authors: Liu Yuanjin , Yao Lixiao , Bu Yue , Sun Qing
Published: August 2021

Epoxy composite materials are widely used in power equipment. As the voltage level increases, the requirement of material properties, including electrical, thermal, and mechanical, has also increased. Introducing thermally conductive nanofiller to the epoxy/liquid rubber composites system is an effective approach to improve heat performance, but the effects of thermally conductive nanofillers on relaxation characteristics remain unclarified. In this paper, nano-alumina (nano-Al2O3) and nano-boron nitride (nano-BN) have been employed to modify the epoxy/carboxyl-terminated liquid nitrile–butadiene rubber (epoxy/CTBN) composites system. The thermal conductivity and glass transition temperature of different formula systems have been measured. The effect of the nanofillers on the relaxation behaviors of the resin matrix has been investigated. Results show that the different kinds of nanofillers will introduce different relaxation processes into the matrix and increase the conductivity at the same time. This study can provide a theoretical basis for the synergistic improvement of multiple properties of epoxy resin composites.
Powder Reuse Cycles in Electron Beam Powder Bed Fusion—Variation of Powder Characteristics
Authors: Shanbhag Gitanjali , Vlasea Mihaela
Published: August 2021

A path to lowering the economic barrier associated with the high cost of metal additively manufactured components is to reduce the waste via powder reuse (powder cycled back into the process) and recycling (powder chemically, physically, or thermally processed to recover the original properties) strategies. In electron beam powder bed fusion, there is a possibility of reusing 95–98% of the powder that is not melted. However, there is a lack of systematic studies focusing on quantifying the variation of powder properties induced by number of reuse cycles. This work compares the influence of multiple reuse cycles, as well as powder blends created from reused powder, on various powder characteristics such as the morphology, size distribution, flow properties, packing properties, and chemical composition (oxygen and nitrogen content). It was found that there is an increase in measured response in powder size distribution, tapped density, Hausner ratio, Carr index, basic flow energy, specific energy, dynamic angle of repose, oxygen, and nitrogen content, while the bulk density remained largely unchanged.