bluuebunny/crossref_metadata_2025 huggingface dataset:
Odontogenic Differentiation-Induced Tooth Regeneration by Psoralea corylifolia L.
Authors: Jang Hye-Ock , Ahn Tea-Young , Ju Ji-Min , Bae Soo-Kyung , Kim Hyung-Ryong , Kim Da-Sol
Published: May 2022

Psoralea corylifolia L. (P. corylifolia) has been used as an oriental phytomedicine to treat coldness of hands and feet in bone marrow injury. Hydroxyapatite is usually used for tooth regeneration. In this study, the role of P. corylifolia and bakuchiol, a compound originated from P. corylifolia as differentiation-inducing substances for tooth regeneration, was determined by monitoring odontogenic differentiation in human dental pulp stem cells (hDPSCs). We confirmed that P. corylifolia extracts and bakuchiol increased the odontogenic differentiation of hDPSCs. In addition, the expression of the odontogenic differentiation marker genes alkaline phosphatase (APL), Runt-related transcription factor 2 (RUNX-2), osteocalcin (OC), and dentin matrix acidic phosphoprotein-1 (DMP-1) was proved by real-time polymerase chain reaction, and protein expression of dentin matrix acidic phosphoprotein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) was proved by western blotting. Further, by confirming the increase in small mothers against decapentaplegia (SMAD) 1/5/8 phosphorylation, the SMAD signaling pathway was found to increase the differentiation of odontoblasts. This study confirmed that P. corylifolia L. extracts and bakuchiol alone promote odontogenic differentiation in hDPSCs. These results suggest that bakuchiol from P. corylifolia is responsible for odontogenic differentiation, and they encourage future in vivo studies on dentin regeneration.
Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature
Authors: Silvestris Erica , Minoia Carla , Guarini Attilio , Opinto Giuseppina , Negri Antonio , Dellino Miriam , Tinelli Raffaele , Cormio Gennaro , Paradiso Angelo Virgilio , De Palma Giuseppe
Published: May 2022

Cancer treatment related infertility (CTRI) affects more than one third of young women undergoing anti-cancer protocols, inducing a premature exhaustion of the ovarian reserve. In addition to ovarian suppression by GnRHa, oocyte and cortex cryopreservation has gained interest in patients with estrogen-sensitive tumors for whom the hormonal burst to prompt the multiple follicular growth could provide a further pro-life tumor pulsing. On the other hand, cortex reimplantation implies a few drawbacks due to the unknown consistency of the follicles to be reimplanted or the risk of reintroducing malignant cells. The capability of ovarian stem cells (OCSs) from fresh ovarian cortex fragments to differentiate in vitro to mature oocytes provides a tool to overcome these drawbacks. In fact, since ovarian cortex sampling and cryopreservation is practicable before gonadotoxic treatments, the recruitment of OSCs from defrosted fragments could provide a novel opportunity to verify their suitability to be expanded in vitro as oocyte like cells (OLCs). Here, we describe in very preliminary experiments the consistency of an OSC population from a single cryopreserved ovarian cortex after thawing as well as both their viability and their suitability to be further explored in their property to differentiate in OLCs, thus reinforcing interest in stemness studies in the treatment of female CTRI.
Assessing Role of Drought Indices in Anticipating Pine Decline in the Sierra Nevada, CA
Authors: Kim Yoonji , Grulke Nancy , Merschel Andrew , Uyeda Kellie
Published: May 2022

Tree mortality in Sierra Nevada’s 2012–2015 drought was unexpectedly excessive: ~152 million trees died. The relative performance of five drought indices (DIs: SPEI, AI, PDSI, scPDSI, and PHDI) was evaluated in the complex, upland terrain which supports the forest and supplies 60% of Californian water use. We tested the relative performance of DIs parameterized with on-site and modeled (PRISM) meteorology using streamflow (linear correlation), and modeled forest stand NDVI and tree basal area increment (BAI) with current and lagged year DI. For BAI, additional co-variates that could modify tree response to the environment were included (crown vigor, point-in-time rate of bole growth, and tree to tree competition). On-site and modeled parameterizations of DIs were strongly correlated (0.9), but modeled parameterizations overestimated water availability. Current year DIs were well correlated (0.7–0.9) with streamflow, with physics-based DIs performing better than pedologically-based DIs. DIs were poorly correlated (0.2–0.3) to forest stand NDVI in these variable-density, pine-dominated forests. Current and prior year DIs were significant covariates in the model for BAI but accounted for little of the variation in the model. In this ecosystem where trees shift seasonally between near-surface to regolithic water, DIs were poorly suited for anticipating the observed tree decline.
Enhanced Anti-Tribocorrosion Performance of Ti-DLC Coatings Deposited by Filtered Cathodic Vacuum Arc with the Optimization of Bias Voltage
Authors: Shen Yongqing , Luo Jun , Liao Bin , Chen Lin , Zhang Xu , Zhao Yuanyuan , Pang Pan , Zeng Xinmiao
Published: May 2022

To improve the anti-tribocorrosion property, and decrease the metal dissolution and wear of stainless-steel components caused by the synergistic action of corrosion and friction in marine environments, Ti-DLC coatings were obtained on steel substrate using a filtered cathodic vacuum arc (FCVA) system by adjusting bias voltage. The structure, mechanical properties, corrosion, and tribocorrosion behavior were investigated. Increasing the bias voltage from −50 V to −300 V, Ti content decreased from 23.9 to 22.5 at.%, and grain size decreased first, and then increased. Obvious TiC grains embedded in the amorphous carbon matrix were observed in the coating from the TEM result. Hardness increased from 30.23 GPa to 34.24 GPa with an increase in bias voltage from −50 to −200 V. The results of tribocorrosion testing showed that the Ti-DLC coatings at −200 V presented the best anti-tribocorrosion performance with the smallest friction coefficient of 0.052, wear rate of 2.48 × 10−7 mm3/N∙m, and high open-circuit potential, which is mainly due to the dense structure, high value of H/E* and H3/E*2, and great corrosion resistance. Obtained results suggest that the Ti-DLC coating with nanocomposite structure is a potential protective material for marine equipment.
Surface Quality Experimental Study on Rotary Ultrasonic Machining of Honeycomb Composites with a Circular Knife Cutting Tool
Authors: Liu Gang , Yang Jie , Zhang Liqiang , Gao Qiuge , Qian Long , Zhang Rongyao
Published: May 2022

Honeycomb composites (HCs) are diversely employed in aerospace, national defense and other fields owing to their remarkable spatial geometry and excellent mechanical properties. Their complex hexagonal cell structure and heterogeneous material properties cause major problems when implementing high-quality processing. Surface defects generated by processing will reduce the capability and service lifespan of the honeycomb sandwich structure. Therefore, the high quality of HCs is a topic of close attention for researchers. In this paper, the consequences of different cutting parameters of rotary ultrasonic machining (RUM) on surface quality with an ultrasonic circular knife (UCK) were studied through multiple groups of single-factor and orthogonal experiments with two-factors/four-levels and one-factor/three-levels. The single factor experiment was used to explain the effect that the degree of cutting parameters has on surface quality, and the orthogonal experiments were applied to explain the interaction between the processing parameters and the influence law of each factor on surface quality. Therefore, the reasonable cutting parameters of HCs were determined through experimental results to provide guidance for the realization of the precise and efficient machining of HCs. This study can provide a basis for the subsequent comprehensive consideration of various factors to achieve high-quality machining of HCs.
Crystal Growth and Characterization of ZrSiS-Type Topological Dirac Semimetals
Authors: Yang Ying , Zhu Peng , Yang Liu , Liu Jinjin , Li Yongkai , Wang Zhiwei
Published: May 2022

WHM materials (W = Zr/Hf, H = Si/Ge/Sn, M = S/Se/Te) represent a large family of topological semimetals, which have attracted intensive interest since they are considered to be good candidates for studying various topological states. Here, we report the crystal growth, characterization, and electronic properties of HfSiS, ZrGeS, and ZrGeSe. All samples were prepared by a chemical vapor transport method with I2 as a transport agent, and the growth conditions were optimized. X-ray diffraction (XRD) measurements showed that the as-grown crystals crystallized in a PbFCl-type layered structure. They all showed metallic behavior from temperature-dependent resistivity measurements and the carrier densities were estimated to be in the order of 1021 cm−3. A large magnetoresistance of up to 1200% and an obvious Shubnikov–de Hass (SdH) oscillation were observed for HfSiS.
Look beyond the Mirror: Laparoscopic Cholecystectomy in Situs Inversus Totalis—A Systematic Review and Meta-Analysis (and Report of New Technique)
Authors: Enciu Octavian , Toma Elena Adelina , Tulin Adrian , Georgescu Dragos Eugen , Miron Adrian
Published: May 2022

Background: Laparoscopic cholecystectomy in situs inversus totalis (SIT) is a technically and physically demanding procedure for surgeons and there is still a lack of consensus regarding the best technical approach in such cases. We conducted a systematic review and meta-analysis to evaluate port placement, the dominant hand of the surgeon, preoperative imaging, morbidity, and mortality. Methods: We searched MEDLINE, SCOPUS, Web of Science, and the Cochrane Library for studies of patients with SIT that underwent laparoscopic cholecystectomy. Of 387 identified records, 101 met our inclusion criteria, all of them case reports or case series of maximum of 6 patients. Results: Out of the 121 patients included in the analysis, 94 were operated on using a “mirrored American” technique, 12 using the “Mirrored French”, 9 employed single-port techniques, and 6 described novel port placements. Even though most surgeries were conducted by a right-handed surgeon (93 cases), surgeries performed by the seven left-handed surgeons yielded shorter intervention times (p = 0.024). Preoperative imaging (CT, MRI, MRCP, ERCP) also correlated with a lower duration of surgery (p = 0.038. Length of stay was associated with the type of disease, but not with other studied endpoints. Morbidity was less than 1%, and conversion rates and mortality were nil. Conclusions: Cholecystectomy in SIT is a safe but challenging procedure and surgeons should prepare in advance for the unfamiliar aspects of completing such a task. While preoperative imaging and a left-handed surgeon are beneficial in terms of surgery length, when these are not available surgeons should focus on achieving the most comfortable setting based on their experience and tailor their approach to the patient at hand. Further studies are needed in order to properly describe and evaluate intraoperative findings as well as surgeon-dependent factors that could improve future recommendations.
Diagnostic Age, Age at Death and Stage Migration in Men Dying with or from Prostate Cancer in Denmark
Authors: Andersen Marc Casper Meineche , Stroomberg Hein Vincent , Brasso Klaus , Helgstrand John Thomas , Røder Andreas
Published: May 2022

The impact of changes in diagnostic activity and treatment options on prostate cancer epidemiology remains a subject of debate. Newly published long-term survival outcomes may not represent contemporary patients and new perspectives are in demand. All men dying in Denmark with prostate cancer diagnosis during a 10-year period were analyzed to address the stage migration of and time lived with prostate cancer diagnosis. All male deaths in Denmark between 2007 and 2016 (n = 261,657) were obtained and crosslinked with The Danish Prostate Cancer Registry (DaPCaR) and the Danish Cancer Registry. Correlation in diagnostic age and stage (localized, locally advanced, metastatic), age at death and cause of death were investigated by Kruskal-Wallis test and linear regression in 15,692 men diagnosed with prostate cancer. Prostate cancer mortality remained stable during the study period. Among the men who died of prostate cancer, 65% had locally advanced or metastatic disease at diagnosis. Age at diagnosis declined in men diagnosed with localized disease and remained constant in men with locally advanced or metastatic disease. Age at death increased in all men. Despite increased efforts to detect prostate cancer early, two-thirds of men who die from prostate cancer still have advanced prostate cancer at the time of diagnosis. Our data show increased life-expectancy in men diagnosed with prostate cancer, however, this benefit must be weighed against increased time of living with the disease and overdiagnosis. The intensified treatment of elderly men and men with advanced disease may be the key to lower prostate cancer mortality.
Thermal Network Model for an Assessment of Summer Indoor Comfort in a Naturally Ventilated Residential Building
Authors: Michalak Piotr
Published: May 2022

Costs of cooling installations cause them to be very rarely used in residential buildings in countries located in heating-dominated climates, like Poland. Hence, there arises the need to assess indoor thermal comfort during summer and to indicate ways to reduce possible overheating. This paper presents an attempt to use the thermal network model of the building zone of EN ISO 13790 to assess indoor operative temperature during four warm months from June to September. The model of the naturally ventilated single-family residential building located in central Poland was used. Performed calculations for the base case resulted in 38 and 63 days within the comfort zone at 80% acceptance level in a total of 122 days in the analyzed period for EN 15251 and ASHRAE standards, respectively. Use of external shading on windows and the roof with lower solar absorptance resulted in 46 and 70 days with acceptable conditions, respectively. Further application of night ventilation resulted in the 38 and 63 days, respectively. From the considered solutions in Polish climate conditions, windows shading seems to be the most efficient solution when controlling indoor comfort in residential buildings with no cooling system. A comparison of hourly operative temperature from that model with the detailed simulation in EnergyPlus showed a strong correlation with R2 = 0.934.
Economic Policy Uncertainty and Energy Prices: Empirical Evidence from Multivariate DCC-GARCH Models
Authors: Ringim Salim Hamza , Alhassan Abdulkareem , Güngör Hasan , Bekun Festus Victor
Published: May 2022

Crude oil and natural gas are crucial to the Russian economy. Therefore, this study examined the interconnections between crude oil price, natural gas price, and Russian economic policy uncertainty (EPU) over the period 1994–2019 using multivariate DCC-MGARCH models. The findings show that there are strong interconnections (co-movement) between the energy prices and EPU in Russia, and that it might be misleading to assume independence or neutrality between the variables. Although Russia is also a crucial player in both the natural gas and the crude oil markets, this study reveals that there is a stronger co-movement of the EPU with gas price than with the oil price. Russia is the largest exporter of natural gas and the second-largest producer; it is plausible that the natural gas price correlates with EPU more than the crude oil price. Further, the correlation between gas price and EPU and the correlation between crude oil price and EPU have similar patterns. Each declines almost in the same period and, equally, increases concurrently. In addition, the results revealed that significant global shocks and crises, such as the 2008 global financial crisis, the 2014–2017 Russian financial crisis, the 9/11 terrorist attack, and the Russo–Ukrainian conflicts, influence the interconnections between the energy prices and Russian EPU.
An Efficient Approach for Peak-Load-Aware Scheduling of Energy-Intensive Tasks in the Context of a Public IEEE Challenge
Authors: Limmer Steffen , Einecke Nils
Published: May 2022

The shift towards renewable energy and decreasing battery prices have led to numerous installations of PV and battery systems in industrial and public buildings. Furthermore, the fluctuation of energy costs is increasing since energy sources based on solar and wind power depend on the weather situation. In order to reduce energy costs, it is necessary to plan energy-hungry activities while taking into account private PV production, battery capacity, and energy market prices. This problem was posed in the 2021 “IEEE-CIS Technical Challenge on Predict + Optimize for Renewable Energy Scheduling”. The target was to solve the two subtasks of forecasting the base load and of computing an optimal schedule of a list of energy intensive activities with inter-dependencies. We describe our approach to this challenge, which resulted in the third place of the leaderboard. For the prediction of the base load, we use a combination of a statistical and a machine learning approach. For the optimization of schedules, we employ a tuned mixed integer linear programming approach. We present a detailed experimental evaluation of the proposed approach on the use case and data provided in the challenge.
Genetic Optimisation of a Free-Stream Water Wheel Using 2D Computational Fluid Dynamics Simulations Points towards Design with Fully Immersed Blades
Authors: Shingala Abhishekkumar , Cleynen Olivier , Jain Aman , Hoerner Stefan , Thévenin Dominique
Published: May 2022

A large-scale two-dimensional computational fluid dynamics study is conducted in order to maximise the power output and smoothness of power delivery of a free-stream water wheel, a low-impact hydropower device. Based on models and methods developed in previous research, the study uses a genetic algorithm to optimise the geometry of a wheel with a given radius and depth, maximising two objective functions simultaneously. After convergence and suitable post-processing, a single optimal design is identified, featuring eight shortened blades that become fully immersed at the nadir point. The design results in a 71% reduction in blade material and a 113% increase in the work ratio while improving the hydraulic power by 8% compared to the previous best design. These characteristics are applied retroactively to a broad family of designs, resulting in significant improvements in performance. Analysis of the resulting designs indicates that when either the hydraulic power coefficient, rotor power coefficient, or work ratio is considered, free-stream water wheels with fully immersed blades, whose power mechanisms are shown to rely on lift, as well as drag, outperform all other designs studied so far.
Acoustic Energy Harvesting of Piezoelectric Ceramic Composites
Authors: Figueroa Jose , Staruch Margo
Published: May 2022

Acoustic energy is an often overlooked but increasingly prevalent source of ambient energy that could be scavenged to power a wide range of devices. Piezoelectric materials are often used, but the tradeoff between acoustic impedance matching and the amount of ceramic piezoelectric material as the active material has not previously been investigated. In this work, commercially available 1–3 dice and fill composites with various fill factors (25%, 45%, and 65% of Pb(Zr,Ti)O3) and different acoustic impedance values were tested using an impedance tube and then modeled using a KLM equivalent circuit model. As expected, a higher amount of ceramic material resulted in a higher acoustic absorption coefficient. Experimentally, the highest fill factor with the highest piezoelectric coefficient also resulted in larger output power at all dB levels, reaching a maximum of 115 nW (84 nW/cm3) at 111 dBSPL for the 65% fill sample. In the model, the 25% fill factor with the best acoustic impedance matching shows the highest expected output power instead, but this discrepancy is most likely due to a lowered piezoelectric coefficient during testing due to the clamping conditions.
Hydrothermal Treatment of Residual Forest Wood (Softwood) and Digestate from Anaerobic Digestion—Influence of Temperature and Holding Time on the Characteristics of the Solid and Liquid Products
Authors: Sailer Gregor , Comi Julian , Empl Florian , Silberhorn Martin , Heymann Valeska , Bosilj Monika , Ouardi Siham , Pelz Stefan , Müller Joachim
Published: May 2022

Hydrothermal treatment (HTT) offers the potential to upgrade low-value biomass such as digestate (DG) or forest residue (FR) by producing solids and liquids for material use or energetic utilization. In this study, microwave-assisted HTT experiments with DG and FR as feedstocks were executed at different temperatures (130, 150, 170 °C) and with different holding times (30, 60, 90 min) to determine the influences on product properties (ash and elemental concentrations, calorific values and chemical compounds). In general, DG and FR reacted differently to HTT. For the DG solids, for instance, the ash concentration was reduced to 8.68%DM at 130 °C (initially 27.67%DM), and the higher heating value increased from 16.55 MJ/kgDM to 20.82 MJ/kgDM at 170 °C, while the FR solids were affected only marginally. Elements with importance for emissions in combustion were leached out in both HTT solids. The DG and FR liquids contained different chemical compounds, and the temperature or holding time affected their formation. Depending on the designated application of HTT, less severe conditions can deliver better results. It was demonstrated that different low-temperature HTT conditions already induce strong changes in the product qualities of DG and FR. Optimized interactions between process parameters (temperature, holding time and feedstock) might lead to better cost–benefit effects in HTT.
The Fouling Effect on Commercial Ceramic Membranes during Filtration of Microalgae Chlorella vulgaris and Monoraphidium contortum
Authors: Nędzarek Arkadiusz , Mitkowski Piotr Tomasz
Published: May 2022

Although interest in the use of membranes for the concentration of microalgal biomass has steadily been growing, little is known regarding the phenomena of membrane fouling. In addition, more attention has been given to polymeric membranes compared to ceramic membranes, which have a longer life that is associated with a higher resistance to aggressive chemical cleaning. In this study, microfiltration (MF) and ultrafiltration (UF) of two microalgae species, Chlorella vulgaris and Monoraphidium contortum, were carried out using tubular crossflow ceramic membranes. Permeate flux was measured, resistance was calculated, and dissolved organic carbon (DOC) was determined. The flux reduction during the first 10 min of filtration was higher for MF than UF (>70% and <50%), and steady-state permeate fluxes were <5% (for MF) and <25% (for UF) of initial (in m3 m−2 s−1) 6.2 × 10−4 (for MF) and 1.7 × 10−4 (for UF). Total resistances (in m−1) were in the ranges of 4.2–5.4 × 1012 (UF) and 2.6–3.1 × 1012 (MF) for M. contortum and C. vulgaris, respectively. DOC reduction was higher for UF membrane (>80%) than for MF (<66%) and DOC concentrations (mg C L−1) in permeates following MF and UF were about five and two, respectively. In conclusion, we demonstrated: (i) higher irreversible resistance for UF and reversible resistance for MF; (ii) permeate flux higher for UF and for M. contortum; (iii) the significant role of dissolved organic compounds in the formation of reversible resistance for MF and irreversible resistance for UF.
Influence of Thermoelectric Properties and Parasitic Effects on the Electrical Power of Thermoelectric Micro-Generators
Authors: El Oualid Soufiane , Kosior Francis , Span Gerhard , Mehmedovic Ervin , Paris Janina , Candolfi Christophe , Lenoir Bertrand
Published: May 2022

Heat recovery systems based on thermoelectric micro-generators (µ-TEGs) can play a significant role in the development of wireless, energetically autonomous electronics. However, to date, the power density recovered for low temperature differences using µ-TEGs is limited to a few micro-watts or less, which is still insufficient to power a wide-range of wireless devices. To develop more efficient µ-TEGs, material, device and system requirements must be considered simultaneously. In this study, an innovative design of an in-plane µ-TEG integrating bismuth telluride forming sinusoidal-shaped trenches is reported. Using 3D numerical modelling, the influence of boundary conditions, parasitic effects (electrical and thermal contact resistances), and transport properties of thermoelectric materials on the output power of these µ-TEGs are investigated in detail for a small temperature difference of 5 K between the hot and cold sources. Compared to wavy-shaped trenches, this novel shape enables enhancing the output power. The results show that either the thermal conductivity or the Seebeck coefficient of the active n- and p-type semiconductors is the key parameter that should be minimized or maximized, depending on the magnitude of the parasitic effects.
An Improved Method of Clay-Induced Rock Typing Derived from Log Data in Modelling Low Salinity Water Injection: A Case Study on an Oil Field in Indonesia
Authors: Zakyan Hafizh , Permadi Asep Kurnia , Pratama Egi Adrian , Naufaliansyah Muhammad Arif
Published: May 2022

Low salinity water injection (LSWI) is an emerging way to improve waterflood performance through chemical processes. The presence of clay minerals is one of the required parameters to successfully implement LSWI in sandstone formations. The ability of clays to exchange the cations, represented by cation exchange capacity (CEC), leads to oil detachment from the rock surface and changes the formation wettability toward water-wet. There are still limited studies that discuss the implementation of specific CEC models in the field-scale LSWI reservoir simulation. This paper attempts to propose an improved method of clay-induced rock typing that can be representatively implemented for field-scale reservoir simulation. The scope of this study is limited to a sandstone reservoir from an oil field in Indonesia. The oil is considered light, and the reservoir contains main clay minerals, including kaolinite and illite, and a trace of chlorite was also found from the XRD evaluation. CEC can be derived from log data, while rock type can also be estimated from log data by using the artificial neural network method. The main finding is that the combination of those variables, i.e., log data, rock properties, and CEC, results in an improved method to characterize and classify the clay into three types associated with conventional rock types. The classification obtained by the clay typing method can be utilized as an input for advanced LSWI modeling, which is expected to provide more robust results. Furthermore, dispersed clay has a strong influence on the magnitude of cation exchange capacity rather than laminar and structural clays.
A Review on Solar Energy Utilization and Projects: Development in and around the UAE
Authors: Ramachandran Tholkappiyan , Mourad Abdel-Hamid I. , Hamed Fathalla
Published: May 2022

Demand on the energy sector has increased significantly due to the incredible evolution of the industry and urbanization. Photovoltaic (PV) technology is rapidly evolving to meet the demands of people in the United Arab Emirates (UAE) by generating more electricity. The UAE has demonstrated that it has the world’s highest rates of sun exposure, indicating a significant efficiency in solar energy development. This might be a way to cut down on fossil fuel consumption and greenhouse gas emissions even further. In this review, we discuss five major aspects of solar energy utilization and projects within the framework of the UAE starting with (i) recent advances in solar scenario and development trends, (ii) electricity production, consumption, and tariffs, (iii) focus on various key aspects of photovoltaic solar installation projects inside the Dubai, Abu Dhabi emirates and other smaller projects in the UAE, (iv) different solar projects outside the UAE, and (v) the solar energy industry, including trading, assembly, manufacturing, installation, and other activities in the UAE. The review finishes with a summary of problems, current developments, and future outlooks.
Invasion of Emerald Ash Borer Agrilus planipennis and Ash Dieback Pathogen Hymenoscyphus fraxineus in Ukraine—A Concerted Action
Authors: Davydenko Kateryna , Skrylnyk Yuriy , Borysenko Oleksandr , Menkis Audrius , Vysotska Natalia , Meshkova Valentyna , Olson Åke , Elfstrand Malin , Vasaitis Rimvys
Published: May 2022

Emerald Ash Borer (EAB), Agrilus planipennis, is a beetle that originates from East Asia. Upon invasion to North America in the early 2000s, it killed untold millions of ash trees. In European Russia, EAB was first detected in Moscow in 2003 and proved to have the potential to also kill native European ash (Fraxinus excelsior). The beetle has since spread in all geographic directions, establishing itself in eastern Ukraine by 2019 and possessing potential for further westward spread towards the EU. Apart from the approaching EAB, F. excelsior is currently threatened by the dieback disease (ADB) caused by the invasive ascomycete fungus Hymenoscyphus fraxineus. The infestation by EAB combined with ADB infection is expected to be more lethal than either of them alone, yet the potential consequences are unknown. To date, eastern Ukraine represents the geographic area in which both invasions overlap, thus providing the opportunity for related investigations. The aims of the study were to investigate: (i) the EAB expansion range in Ukraine, (ii) the relative susceptibility of F. excelsior and American ash (Fraxinus pennsylvanica) to EAB and ADB, and (iii) the combined effect/impact on ash condition imposed by both the pest and disease in the area subjected to the invasion. The results have demonstrated that (i) the invasion of EAB is currently expanding both in terms of newly infested trees and invaded geographic area; (ii) F. excelsior is more resistant to EAB than F. pennsylvanica, while F. excelsior is more susceptible to ADB than F. pennsylvanica; and (iii) the infection by ADB is likely to predispose F. excelsior to the infestation by EAB. It was concluded that inventory and mapping of surviving F. excelsior, affected by both ADB and EAB, is necessary to acquire genetic resources for the work on strategic, long-term restoration of F. excelsior in devastated areas, thereby tackling a possible invasion of EAB to the EU.
Two Nothofagus Species in Southernmost South America Are Recording Divergent Climate Signals
Authors: Soto-Rogel Pamela , Aravena Juan Carlos , Villalba Ricardo , Bringas Christian , Meier Wolfgang Jens-Henrik , Gonzalez-Reyes Álvaro , Grießinger Jussi
Published: May 2022

Recent climatic trends, such as warming temperatures, decrease in rainfall, and extreme weather events (e.g., heatwaves), are negatively affecting the performance of forests. In northern Patagonia, such conditions have caused tree growth reduction, crown dieback, and massive die-back events. However, studies looking at these consequences in the southernmost temperate forest (Nothofagus betuloides and Nothofagus pumilio) are much scarcer, especially in southernmost South America (SSA). These forests are also under the influence of the positive phase of Antarctic Oscillation (AAO, also known as Southern Annular Mode, SAM) that has been associated with increasing trends in temperature, drought, and extreme events in the last decades. This study evaluated the growth patterns and the climatic response of eight new tree-ring chronologies from Nothofagus species located at the upper treeline along different environmental gradients in three study areas: Punta Arenas, Yendegaia National Park, and Navarino Island in SSA. The main modes of the ring-width index (RWI) variation were studied using principal component analysis (PCA). We found that PC1 has the higher loadings for sites with precipitation values over 600 mm/yr, PC2 with N. betuloides sites, and PC3 with higher loadings for sites with precipitation values below 600 mm/yr. Our best growth-climate relationships are between N. betuloides and AAO and the most northeastern site of N. pumilio with relative humidity (which coincides with heatwaves and extreme drought). The climatic signals imprinted in the southernmost forests are sensitive to climatic variability, the climate forcing AAO, and the effects of climate change in the last decades.
Study of Fermentation Strategies by Lactobacillus gasseri for the Production of Probiotic Food Using Passion Fruit Juice Combined with Green Tea as Raw Material
Authors: Lima Wanessa Dayane Leite , Monteiro Shênia Santos , Pasquali Matheus Augusto de Bittencourt
Published: May 2022

Foods fermented by Lactobacillus with probiotic properties convey health benefits to consumers, in addition to fulfilling the basic function of nourishing. This work aimed to evaluate the growth characteristics of L. gasseri in passion fruit juice and passion fruit added with green tea. Fermentation under evaluation of different pH (3.5–7.5), temperature (30–44 °C), and with the addition of green tea (7.5–15%), took place for 48 h. The results showed that a pH of 7.5 and temperature of 44 °C showed higher cell production, and it was also verified that the addition of 15% of green tea induced the growth of L. gasseri in passion fruit juice. The concentrations of probiotic cells observed were above 9 Log CFU.mL−1 and, therefore, they are promising products for consumption as a functional food and application in the food industry with potential health benefits.
A dPCR Method for Quantitative Authentication of Wild Lingonberry (Vaccinium vitis-idaea) versus Cultivated American Cranberry (V. macrocarpon)
Authors: Karppinen Katja , Avetisyan Anna , Hykkerud Anne Linn , Jaakola Laura
Published: May 2022

Berries of the genus Vaccinium are highly valued health-beneficial superfoods, which are commonly subjected to adulteration and mixed with each other, or with other common berry species. A quantitative DNA-based method utilizing a chip-based digital polymerase chain reaction (dPCR) technique was developed for identifying and quantifying wild lingonberry (V. vitis-idaea) and cultivated American cranberry (V. macrocarpon). The dPCR method with species-specific primers for mini-barcoding was designed based on the indel regions found in the trnI-CAU–trnL-CAA locus in the chloroplast genome. The designed primers were able to amplify only target species, enabling to distinguish the two closely related species with good sensitivity. Our results illustrated the ability of the method to identify lingonberry and American cranberry DNA using PCR without the need for probes or further sequencing. The dPCR method could also quantify the DNA copy number in mixed samples. Based on this study, the method provides a basis for a simple, fast, and sensitive quantitative authentication analysis of lingonberry and American cranberry by dPCR. Moreover, it can also provide a platform for authentication analyses of other plant species as well by utilizing the indel regions of chloroplast genomes.
Comparison and Optimization of Different Extraction Methods of Bound Phenolics from Jizi439 Black Wheat Bran
Authors: Chen Xi , Sun Kuijie , Zhuang Kun , Ding Wenping
Published: May 2022

Diet rich in phenolics would potentially associate with multiple health benefits. Response surface methodology (RSM) was introduced to optimize the process of ultrasound- and microwave-assisted extraction of bound phenolics from the bran of a newly developed black wheat breeding line Jizi439 and then compared with the traditional alkaline method. The optimum conditions were found to be 66 °C, 48 min, and power 240 W for ultrasound-assisted extraction (UAE), and 120 s, power 420 W for microwave-assisted extraction (MAE), respectively. Total bound phenolic contents (TBPCs), determined by Folin-Ciocalteu reagent, were 8466.7 ± 240.9 μg gallic acid equivalents per gram (μg GAE/g) bran for UAE and 8340.7 ± 146.7 μg GAE/g bran for MAE under optimized conditions, which were both significantly higher than that of the traditional method (5688.9 ± 179.6 μg GAE/g) (p < 0.05). Antioxidant activities (AAs) were determined by DPPH and ABTS methods. UAE extracts showed the highest DPPH scavenging activity (77.5 ± 0.9%), while MAE extracts showed the highest ABTS scavenging activity (72.1 ± 0.6%). Both were significantly higher than that of the traditional method (69.6 ± 1.1% for DPPH and 65.9 ± 0.5% for ABTS) (p < 0.05). Total bound phenolics (TBPs) profiles were further analyzed by HPLC, and results indicated that ferulic acid was dominant, followed by vanillic acid and p-coumaric acid. The contents of each identified individual phenolics were significantly increased by ultrasound and microwave. In conclusion, UAE and MAE were comparable with each other in TBP yields and AAs; however, when taking operation time and energy consumption into consideration, MAE was more efficient than UAE. Our study suggested efficiency extraction methods for further use of bound phenolics as a healthy food ingredient.
Business Model Analysis of Smart City Logistics Solutions Using the Business Model Canvas: The Case of an On-Demand Warehousing E-Marketplace
Authors: Parodos Leonidas , Tsolakis Orestis , Tsoukos George , Xenou Elpida , Ayfantopoulou Georgia
Published: May 2022

The rapid rise of e-commerce and advances in the technological sector have enabled the emergence of modern business models on e-marketplaces. Digital and on-demand e-marketplaces have become increasingly popular, responding to the trend of the sharing economy in various business sectors, but there is little guidance on how to develop the respective business models in order to ensure the long-term sustainability of such companies. The present paper is based upon the development and operation of an innovative on-demand warehousing e-marketplace in Greece as a one stop-shop for on-demand warehousing services. The purpose of this paper is to identify the necessary components for developing a successful innovative business model for a viable and effective on-demand warehousing platform. The identification of the value proposition of the proposed e-marketplace, the necessary business infrastructure and the customer interface are described, analyzed and adapted to the Greek Market. Furthermore, this paper also describes the cost structure and the revenue streams of the proposed on-demand warehousing e-marketplace.
Preparation of Chitosan/Recombinant Human Collagen-Based Photo-Responsive Bioinks for 3D Bioprinting
Authors: Yang Yang , Wang Zixun , Xu Yuanyuan , Xia Jingjing , Xu Zhaoxian , Zhu Shuai , Jin Mingjie
Published: May 2022

Collagen and chitosan are frequently used natural biomaterials in tissue engineering. However, most collagen is derived from animal tissue, with inconsistent quality and pathogen transmittance risks. In this context, we aimed to use a reliable Type-III recombinant human collagen (RHC) as an alternative biomaterial together with chitosan to develop novel photo-responsive bioinks for three-dimensional (3D) bioprinting. RHC was modified with methacrylic anhydride to obtain the RHC methacryloyl (RHCMA) and mixed with acidified chitosan (CS) to form composites CS-RHCMA. The characterizations demonstrated that the mechanical properties and the degradation of the bioinks were tunable by introducing the CS. The printabilities improved by adding CS to RHCMA, and various structures were constructed via extrusion-based 3D printing successfully. Moreover, in vitro tests confirmed that these CS-RHCMA bioinks were biocompatible as human umbilical vein endothelial cells (HUVECs) were sustained within the constructs post-printing. The results from the current study illustrated a well-established bioinks system with the potential to construct different tissues through 3D bioprinting.
Deep Splicer: A CNN Model for Splice Site Prediction in Genetic Sequences
Authors: Fernandez-Castillo Elisa , Barbosa-Santillán Liliana Ibeth , Falcon-Morales Luis , Sánchez-Escobar Juan Jaime
Published: May 2022

Many living organisms have DNA in their cells that is responsible for their biological features. DNA is an organic molecule of two complementary strands of four different nucleotides wound up in a double helix. These nucleotides are adenine (A), thymine (T), guanine (G), and cytosine (C). Genes are DNA sequences containing the information to synthesize proteins. The genes of higher eukaryotic organisms contain coding sequences, known as exons and non-coding sequences, known as introns, which are removed on splice sites after the DNA is transcribed into RNA. Genome annotation is the process of identifying the location of coding regions and determining their function. This process is fundamental for understanding gene structure; however, it is time-consuming and expensive when done by biochemical methods. With technological advances, splice site detection can be done computationally. Although various software tools have been developed to predict splice sites, they need to improve accuracy and reduce false-positive rates. The main goal of this research was to generate Deep Splicer, a deep learning model to identify splice sites in the genomes of humans and other species. This model has good performance metrics and a lower false-positive rate than the currently existing tools. Deep Splicer achieved an accuracy between 93.55% and 99.66% on the genetic sequences of different organisms, while Splice2Deep, another splice site detection tool, had an accuracy between 90.52% and 98.08%. Splice2Deep surpassed Deep Splicer on the accuracy obtained after evaluating C. elegans genomic sequences (97.88% vs. 93.62%) and A. thaliana (95.40% vs. 94.93%); however, Deep Splicer’s accuracy was better for H. sapiens (98.94% vs. 97.15%) and D. melanogaster (97.14% vs. 92.30%). The rate of false positives was 0.11% for human genetic sequences and 0.25% for other species’ genetic sequences. Another splice prediction tool, Splice Finder, had between 1% and 3% of false positives for human sequences, while other species’ sequences had around 4% and 10%.
Novel Loss-of-Function Variants in CHD2 Cause Childhood-Onset Epileptic Encephalopathy in Chinese Patients
Authors: Wang Xu , Cui Di , Ding Changhong , Chen Chunhong , Wang Xiaohui , Fang Fang , Jin Hong , Ren Xiaotun
Published: May 2022

Developmental and epileptic encephalopathy-94 (DEE94) is a severe form of epilepsy characterized by a broad spectrum of neurodevelopmental disorders. It is caused by pathogenic CHD2 variants. While only a few pathogenic CHD2 variants have been reported with detailed clinical phenotypes, most of which lack molecular analysis. In this study, next-generation sequencing (NGS) was performed to identify likely pathogenic CHD2 variants in patients with epilepsy. Three likely pathogenic variants were finally identified in different patients. The seizure onset ages were from two years to six years. Patients 1 and 2 had developmental delays before epilepsy, while patient 3 had intellectual regression after the first seizure onset. The observed seizures were myoclonic, febrile, and generalized tonic-clonic, which had been controlled by different combinations of antiepileptic drugs. Two de novo (c.1809_1809+1delGGinsTT, p.? and c.3455+2_3455+3insTG, p.?) and one maternal (c.3783G>A, p.W1261*) variant were identified, which were all predicted to be pathogenic/likely pathogenic. Molecular analysis was performed in patient 1, and we detected aberrantly spliced products, proving the pathogenicity of this CHD2 variant. New cases with novel variants, along with a detailed clinical and molecular analysis, are important for a better understanding of CHD2-related epileptic encephalopathy.
Obstructive Sleep Apnea and Adherence to Continuous Positive Airway Pressure (CPAP) Treatment: Let’s Talk about Partners!
Authors: Rosa Debora , Amigoni Carla , Rimoldi Elisa , Ripa Paola , Ligorio Antonella , Fracchiolla Miriam , Lombardi Carolina , Parati Gianfranco , Perger Elisa
Published: May 2022

Background: Continuous positive airway pressure (CPAP) is the gold standard treatment for obstructive sleep apnea (OSA). The benefits of this therapy were studied and analyzed over time; patient adherence is often poor, as many factors negatively influence it. A topic that needs clarification is whether adherence to CPAP treatment in a patient with OSA is influenced by the behavior of a partner or spouse. Methods: A scoping review was conducted to evaluate the role of partner involvement in the CPAP treatment management in a patient with OSA. The research project was performed between August and September 2021 by consulting the main biomedical databases: CINHAL, Embase, PsycINFO, and PubMed. Results: Among 21 articles considered valid for our aim, 15 are qualitative studies, 5 are quantitative and 1 presents a mixed method. We identified several thematic areas and “key” elements, which are prevalent in the studies evaluated. Conclusions: The presence of a partner promotes adherence to CPAP therapy in patients with OSA, resulting in ameliorating their overall quality of life. To increase CPAP adherence, a trained nurse could represent a reference figure to technically and emotionally support couples during the adaptation period and in the long term.
Personal Protection Equipment and Infection Control Procedures among Health Workers during the COVID-19 Pandemic
Authors: Carmagnola Daniela , Toma Marilisa , Henin Dolaji , Perrotta Mariachiara , Pellegrini Gaia , Dellavia Claudia
Published: May 2022

Health workers have been particularly affected by the COVID-19 pandemic, but it is unclear which healthcare professions are more exposed. We search for information that can help identify health workers who are employed in exposure-prone professions and who, therefore, routinely wear and adopt strict infection control equipment and measures from other colleagues. Our purpose is to test the hypothesis that health professionals historically considered less exposure-prone are affected more severely by COVID-19. Taking dentists as an example of exposure-prone healthcare professionals, this study aims to analyze data on COVID-19-related deaths reported by the Italian board of doctors and dentists’ database to evaluate the number of COVID-19-related deaths of doctors and dentists in Italy from the beginning of the pandemic to 31 December 2022. As of 31 December 2021, out of 364 deaths, 38 were dentists, and of the remaining 326 doctors, 140 were general practitioners (GPs). The percentage of deaths among dentists, total doctors and GPs results in 0.06%, 0.09% and 0.33%, respectively, for the whole sample. Excluding subjects over 70 years of age, the corresponding values are 0.05%, 0.06% and 0.25%. Most of the deaths occurred in Lombardia, and the geographical distribution overlaps the trend of the corresponding general Italian population. Considering the outcome of “death”, dentists, despite being at high risk, are not particularly affected by COVID-19.