bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary insight into the reproductive traits of the flapper skate Dipturus intermedius using in-field ultrasonography and circulating hormone concentrations
Authors: Thorburn J , Cole G , Naylor A , Garbett A , Wilson K , James M , Dodd J , Houghton JDR , Collins PC
Published: July 2023

Due to global population declines, there is a pressing need for data on the life history traits of many elasmobranch species to support the development of species-specific management plans. A lack of information on the reproductive cycle of the Critically Endangered flapper skate Dipturus intermedius was recently identified as a hindrance to its conservation. To address this data gap, we combined non-lethal ultrasound and hormone analysis to investigate the size at maturity and reproductive cycle of the flapper skate in the Loch Sunart to the Sound of Jura Marine Protected Area off the west coast of Scotland. In-field ultrasound imagery revealed encapsulated eggs in utero and was used to determine the presence and size of ovarian follicles. Combining these images with levels of plasma testosterone, progesterone and oestradiol provided valuable insights into the timing of the reproductive cycle and maturity state of the flapper skate. This preliminary study suggests that male skate start to mature at 165 cm and females at 203 cm total length. Oestradiol appears to be the primary hormone controlling the female reproductive cycle and, along with ultrasound images, indicates that females lay pairs of eggs throughout a winter egg-laying season. Our study further highlights how non-lethal methods can be used to investigate the life history of oviparous elasmobranchs in the field. This information will support the identification of important life history groups and their associated habitats and contribute to the development of management strategies for these species.
Hepaticus encephalopathia
Authors: Hunyady Béla , Horváth Gábor
Published: July 2023

Ez az összefoglaló közlemény a krónikus májbetegségek előrehaladott stádiumában gyakran fellépő egyik súlyos komplikáció, a hepaticus encephalopathia klinikai jellemzőivel, diagnosztikájával, ellátásával foglalkozik. A potenciálisan reverzibilis állapot az érdemi klinikai tünettel nem járó működészavartól a májkómáig terjedő, széles skálán mozgó központi idegrendszeri működészavart jelent, a legkülönbözőbb neurológiai és/vagy pszichiátriai manifesztációkkal. Jelentősen rontja az életminőséget és az életkilátásokat, és mind az érintettek, mind a környezetükben élők, mind pedig az ellátórendszerek számára jelentős terhet jelent. A kórképpel és ellátásával kapcsolatban több szempontot megjelenítő összefoglalók és ajánlások láttak napvilágot. Patológiája nem teljesen ismert, nevezéktana és diagnosztikája nem teljesen egységes, megelőzése és terápiája nem teljesen megoldott. Abban viszont teljes a szakmai egyetértés, hogy megjelenése kedvezőtlen prognosztikai jel, fontos a korai felismerése, és elengedhetetlen a tünetekkel járó megjelenési formák és a kiváltó okok mielőbbi hatékony kezelése.
Multi-level ultra-deep fault-controlled karst reservoirs characterization methods for the Shunbei field
Authors: Ma QiQi , Duan Taizhong
Published: July 2023

Ultra-deep carbonate fault-controlled reservoirs are characterized by large burial depths, low dissolution degrees, strong heterogeneity, and limited effective well data. Accurate 3D characterization based on seismic data is essential for efficient development of this type of reservoir. However, traditional seismic prediction methods are insufficient to accurately characterize different reservoir levels in the exploration and development of fault-controlled ultra-deep reservoirs. We propose a set of improved multi-level characterization methods for fault-controlled reservoirs. The improved methods could recover seismic information obscured by strong reflections and reduce the uncertainty of seismic interpretation. This study combined seismic strong reflection suppression and sedimentary strata seismic reflection interference elimination, proposed improved inversion methods, and advanced attribute calculation methods to improve the identification accuracy of reservoirs. In particular, we proposed the karst cave carving method based on improved inversion method and karst cave enhancement algorithm, the dissolved pore zone identification method based on optimization energy envelope algorithm, and the fault-fracture zone characterization method based on optimized atomic decomposition texture contrast. These methods were thoroughly validated by theoretical 3D models and field data. The proposed multi-level characterization methods can effectively improve the identification accuracy of fault-controlled karst reservoirs, provide a benchmark for predicting similar strong heterogeneous carbonate reservoirs, and provide reliable support for further facies modeling.
State of the art in childhood nephrotic syndrome: concrete discoveries and unmet needs
Authors: Vincenti Flavio , Angeletti Andrea , Ghiggeri Gian Marco
Published: July 2023

Nephrotic syndrome (NS) is a clinical entity characterized by proteinuria, hypoalbuminemia, and peripheral edema. NS affects about 2–7 per 100,000 children aged below 18 years old yearly and is classified, based on the response to drugs, into steroid sensitive (SSNS), steroid dependent, (SDNS), multidrug dependent (MDNS), and multidrug resistant (MRNS). Forms of NS that are more difficult to treat are associated with a worse outcome with respect to renal function. In particular, MRNS commonly progresses to end stage renal failure requiring renal transplantation, with recurrence of the original disease in half of the cases. Histological presentations of NS may vary from minimal glomerular lesions (MCD) to focal segmental glomerulosclerosis (FSGS) and, of relevance, the histological patterns do not correlate with the response to treatments. Moreover, around half of MRNS cases are secondary to causative pathogenic variants in genes involved in maintaining the glomerular structure. The pathogenesis of NS is still poorly understood and therapeutic approaches are mostly based on clinical experience. Understanding of pathogenetic mechanisms of NS is one of the ‘unmet needs’ in nephrology and represents a significant challenge for the scientific community. The scope of the present review includes exploring relevant findings, identifying unmet needs, and reviewing therapeutic developments that characterize NS in the last decades. The main aim is to provide a basis for new perspectives and mechanistic studies in NS.
Integrating scRNA and bulk-RNA sequencing develops a cell senescence signature for analyzing tumor heterogeneity in clear cell renal cell carcinoma
Authors: Gong Qiming , Jiang Yan , Xiong Junfeng , Liu Fahui , Guan Jikui
Published: July 2023

Introduction

Cellular senescence (CS) plays a critical role in cancer development, including clear cell renal cell carcinoma (ccRCC). Traditional RNA sequencing cannot detect precise molecular composition changes within tumors. This study aimed to analyze cellular senescence’s biochemical characteristics in ccRCC using single RNA sequencing (ScRNA-seq) and traditional RNA sequencing (Bulk RNA-seq).

Methods

Researchers analyzed the biochemical characteristics of cellular senescence in ccRCC using ScRNA-seq and Bulk RNA-seq. They combined these approaches to identify differences between malignant and non-malignant phenotypes in ccRCC across three senescence-related pathways. Genes from these pathways were used to identify molecular subtypes associated with senescence, and a new risk model was constructed. The function of the gene DUSP1 in ccRCC was validated through biological experiments.

Results

The combined analysis of ScRNA-seq and Bulk RNA-seq revealed significant differences between malignant and non-malignant phenotypes in ccRCC across three senescence-related pathways. Researchers identified genes from these pathways to identify molecular subtypes associated with senescence, constructing a new risk model. Different subgroups showed significant differences in prognosis level, clinical stage and grade, immune infiltration, immunotherapy, and drug sensitivity.

Discussion

Senescence signature markers are practical biomarkers and predictors of molecular typing in ccRCC. Differences in prognosis level, clinical stage and grade, immune infiltration, immunotherapy, and drug sensitivity between different subgroups indicate that this approach could provide valuable insights into senescence-related treatment options and prognostic assessment for patients with ccRCC. The function of the gene DUSP1 in ccRCC was validated through biological experiments, confirming its feasibility as a novel biomarker for ccRCC. These findings suggest that targeted therapies based on senescence-related mechanisms could be an effective treatment option for ccRCC.
On dynamical downscaling of ENSO-induced oceanic anomalies off Baja California Peninsula, Mexico: role of the air-sea heat flux
Authors: Rivas David , Counillon François , Keenlyside Noel
Published: July 2023

The El Niño Southern Oscillation (ENSO) phenomenon is responsible for important physical and biogeochemical anomalies in the Northeastern Pacific Ocean. The event of 1997-98 has been one of the most intense in the last decades and it had large implications for the waters off Baja California (BC) Peninsula with a pronounced warm sea surface temperature (SST) anomaly adjacent to the coast. Downscaling of reanalysis products was carried out using a mesoscale-resolving numerical ocean model to reproduce the regional SST anomalies. The nested model has a 9 km horizontal resolution that extend from Cabo Corrientes to Point Conception. A downscaling experiment that computes surface fluxes online with bulk formulae achieves a better representation of the event than a version with prescribed surface fluxes. The nested system improves the representation of the large scale warming and the localized SST anomaly adjacent to BC Peninsula compared to the reanalysis product. A sensitivity analysis shows that air temperature and to a lesser extent wind stress anomalies are the primary drivers of the formation of BC temperature anomaly. The warm air-temperature anomalies advect from the near-equatorial regions and the central north Pacific and is associated with sea-level pressure anomalies in the synoptic-scale atmospheric circulation. This regional warm pool has a pronounced signature on sea level anomaly in agreement with observations, which may have implications for biogeochemistry.
Assessing the contribution of atmospheric transport to phosphorus in the East China Sea using the oxygen isotope in phosphate
Authors: Zhao Xiuyi , Tian Rongxiang , Feng Wuhu , Jin Zhan
Published: July 2023

Phosphorus is an important nutrient for the growth of marine organisms. External inputs of phosphorus can lead to changes in the primary productivity and may trigger harmful algal blooms. However, few studies have focused on the atmospheric transport of phosphorus to the ocean. We measured the oxygen isotopes in phosphate (δ18Op) and quantitatively determined the contribution of phosphate from atmosphere sources using seawater samples from the East China Sea, a two-component mixing model and a Bayesian isotope mixing model. Our results showed that the δ18Op value retains the signal of the original source and can be used to trace the source of phosphate. The main sources of phosphate in the northeastern East China Sea were from atmospheric inputs, with the contribution of terrigenous phosphate accounting for 56 – 78% of the total. Our findings highlight the importance of the atmospheric transport of phosphate to the ocean, which is important for exploring the mechanisms of harmful algal blooms and in disaster prevention and mitigation.
Research on torsional stiffness of flexspline-flexible bearing contact pair in harmonic drive based on macro-micro scale modeling
Authors: Hu Qiushi , Li Heng , Wang Guang , Li Lei
Published: July 2023

The flexspline and flexible bearing constitute a critical contact pair in a harmonic drive system, and their torsional stiffness has a significant impact on the performance characteristics manifested by the harmonic drive. In this study, a micro scale three-dimensional fractal model was combined with a macro scale finite element simulation method to establish an equivalent torsional stiffness model for the flexspline-flexible bearing contact pair (FS-FB contact pair), which enables the theoretical prediction of the torsional stiffness of this contact pair. A torsional stiffness testing platform was constructed for a harmonic drive, and the consistency between the experimental results of the torsional stiffness curve and the theoretical predictions validates the effectiveness of the proposed model. The influences of torque, installation eccentricity, and deformation coefficient on the torsional stiffness of the FS-FB contact pair were also discussed. The results indicate that the torsional stiffness of the FS-FB contact pair increases nonlinearly with an increase in torque. On the other hand, the torsional stiffness of the FS-FB contact pair decreases with an increase in installation eccentricity, and increases before subsequently decreasing with an increase in deformation coefficient. Moreover, as torque increases, the impact of installation eccentricity and deformation coefficient on the torsional stiffness diminishes. This article provides a theoretical reference for the optimization design and performance enhancement of harmonic drives.
Lowering maintenance immune suppression in elderly kidney transplant recipients; connecting the immunological and clinical dots
Authors: Betjes Michiel G. H. , De Weerd Annelies
Published: July 2023

The management of long-term immune suppressive medication in kidney transplant recipients is a poorly explored field in the area of transplant medicine. In particular, older recipients are at an increased risk for side effects and have an exponentially increased risk of infection-related death. In contrast, an aged immune system decreases the risk of acute T-cell-mediated rejection in older recipients. Recent advances in alloimmunity research have shown a rapid and substantial decline in polyfunctional, high-risk CD4+ T cells post-transplantation. This lowers the direct alloreactivity responsible for T-cell-mediated rejection, also known as donor-specific hyporesponsiveness. Chronic antibody-mediated rejection (c-aABMR) is the most frequent cause of kidney graft loss in the long term. However, in older adults, c-aABMR as a cause of graft loss is outnumbered by death with a functioning graft. In addition, DSA development and a diagnosis of c-aABMR plateau ~10 years after transplantation, resulting in a very low risk for rejection thereafter. The intensity of immune suppression regimes could likely be reduced accordingly, but trials in this area are scarce. Tacrolimus monotherapy for 1 year after transplantation seems feasible in older kidney transplant recipients with standard immunological risk, showing the expected benefits of fewer infections and better vaccination responses.
Prognostic model for hepatocellular carcinoma based on anoikis-related genes: immune landscape analysis and prediction of drug sensitivity
Authors: Zhang Dengyong , Liu Sihua , Wu Qiong , Ma Yang , Zhou Shuo , Liu Zhong , Sun Wanliang , Lu Zheng
Published: July 2023

Background

Hepatocellular carcinoma (HCC) represents a complex ailment characterized by an unfavorable prognosis in advanced stages. The involvement of immune cells in HCC progression is of significant importance. Moreover, metastasis poses a substantial impediment to enhanced prognostication for HCC patients, with anoikis playing an indispensable role in facilitating the distant metastasis of tumor cells. Nevertheless, limited investigations have been conducted regarding the utilization of anoikis factors for predicting HCC prognosis and assessing immune infiltration. This present study aims to identify hepatocellular carcinoma-associated anoikis-related genes (ANRGs), establish a robust prognostic model for HCC, and delineate distinct immune characteristics based on the anoikis signature. Cell migration and cytotoxicity experiments were performed to validate the accuracy of the ANRGs model.

Methods

Consensus clustering based on ANRGs was employed in this investigation to categorize HCC samples obtained from both TCGA and Gene Expression Omnibus (GEO) cohorts. To assess the differentially expressed genes, Cox regression analysis was conducted, and subsequently, prognostic gene signatures were constructed using LASSO-Cox methodology. External validation was performed at the International Cancer Genome Conference. The tumor microenvironment (TME) was characterized utilizing ESTIMATE and CIBERSORT algorithms, while machine learning techniques facilitated the identification of potential target drugs. The wound healing assay and CCK-8 assay were employed to evaluate the migratory capacity and drug sensitivity of HCC cell lines, respectively.

Results

Utilizing the TCGA-LIHC dataset, we devised a nomogram integrating a ten-gene signature with diverse clinicopathological features. Furthermore, the discriminative potential and clinical utility of the ten-gene signature and nomogram were substantiated through ROC analysis and DCA. Subsequently, we devised a prognostic framework leveraging gene expression data from distinct risk cohorts to predict the drug responsiveness of HCC subtypes.

Conclusion

In this study, we have established a promising HCC prognostic ANRGs model, which can serve as a valuable tool for clinicians in selecting targeted therapeutic drugs, thereby improving overall patient survival rates. Additionally, this model has also revealed a strong connection between anoikis and immune cells, providing a potential avenue for elucidating the mechanisms underlying immune cell infiltration regulated by anoikis.
LolA and LolB from the plant-pathogen Xanthomonas campestris forms a stable heterodimeric complex in the absence of lipoprotein
Authors: Furlanetto Valentina , Divne Christina
Published: July 2023

The Gram-negative bacterium Xanthomonas campestris is one of the most problematic phytopathogens, and especially the pathovar campestris (Xcc) that causes a devastating plant disease known as black rot and it is of considerable interest to understand the molecular mechanisms that enable virulence and pathogenicity. Gram-negative bacteria depend on lipoproteins (LPs) that serve many important functions including control of cell shape and integrity, biogenesis of the outer membrane (OM) and establishment of transport pathways across the periplasm. The LPs are localized to the OM where they are attached via a lipid anchor by a process known as the localization of lipoprotein (Lol) pathway. Once a lipid anchor has been synthesized on the nascent LP, the Lol pathway is initiated by a membrane-bound ABC transporter that extracts the lipid anchor of the LP from the IM. The ABC extractor presents the extracted LP to the transport protein LolA, which binds the anchor and thereby shields it from the hydrophilic periplasmic milieu. It is assumed that LolA then carries the LP across the periplasm to the OM. At the periplasmic face of the OM, the LP cargo is delivered to LolB, which completes the Lol pathway by inserting the LP anchor in the inner leaflet of the outer membrane. Earlier studies have shown that loss of Xcc LolA or LolB leads to decreased virulence and pathogenicity during plant infection, which motivates studies to better understand the Lol system in Xcc. In this study, we report the first experimental structure of a complex between LolA and LolB. The crystal structure reveals a stable LolA-LolB complex in the absence of LP. The structural integrity of the LP-free complex is safeguarded by specific protein–protein interactions that do not coincide with interactions predicted to participate in lipid binding. The results allow us to identify structural determinants that enable Xcc LolA to dock with LolB and initiate LP transfer.
Co-circulation and co-infection of hantaviruses and Wenzhou mammarenavirus in small mammals and humans in Jiangxi, China
Authors: Du Shanshan , Xie Yun , Deng Xuefei , Xia Zhiyong , Wu Wei , Huang Xiaoxia , Chen Zhao , Li Aqian , Li Chuan , Wang Qin , Sun Lina , Guo Meijun , Wang Shiwen , Liang Mifang , Li Dexin , Liu Xiaoqing , Li Jiandong
Published: July 2023

Both Orthohantaviruses (HV) and Whenzhou Mammarenaviruses (WENV) are rodents borne viruses, allowing them to spread simultaneously in the same area and infect humans. To explore the potential threat of HV and WENV to public health safety, an environmental and laboratory investigation was conducted in 2020–2021, in Jiangxi province, China. A total of 461 small mammals of 7 species and paired sera from 43 suspected HFRS cases were collected from Jiangxi Province, China. Viral genomic RNA and specific antibodies against HV and WENV were detected to evaluate the epidemic situation of the two viruses. Hantaan virus (HTNV), seoul virus (SEOV) and WENV RNA were detected in the lungs of the captured mammals, which resulted 4.1% and 7.4% of HV and WENV RNA positive respectively. Co-infections of WENV and SEOV were detected from Rattus norvegicus, Mus musculus and Rattus flavipectus with an overall co-infection rate of 0.65%. The detection rates of antibodies in the blood against HV and WENV were 11.9% (55/461), and 13.2% (61/461) respectively. The prevalence of viral infection and viral genetic characters varied among the selected areas. In the paired sera of 43 suspected HFRS cases, 38 were with HV infection, 11 were with WENV IgG, and 7 with a 4-fold or more of WENV IgG titer elevation. These results revealed the fact of the co-circulating and coinfection of HV and WENV in the same area at the same time, which might impact on public health safety.
Clinical application of recommendations for neurobehavioral assessment in disorders of consciousness: an interdisciplinary approach
Authors: Murtaugh Brooke , Shapiro Rosenbaum Amy
Published: July 2023

Accurate diagnosis, prognosis, and subsequent rehabilitation care planning for persons with Disorders of Consciousness (DoC) has historically posed a challenge for neurological care professionals. Evidence suggests rates of misdiagnosis may be as high as 40% when informal beside evaluations are used to determine level of consciousness. The presence of myriad medical, neurological, functional (motor, sensory, cognitive) and environmental confounds germane to these conditions complicates behavioral assessment. Achieving diagnostic certainty is elusive but critical to inform care planning, clinical decision making, and prognostication. Standardized neurobehavioral rating scales has been shown to improve accuracy in distinguishing between coma, unresponsive wakefulness syndrome/vegetative state and minimally consciousness state as compared to informal assessment methods. Thus, these scales are currently recommended for use as the informal “gold standard” for diagnostic assessment in DoC. The following paper will present an evidence-based approach to neurobehavioral assessment for use in clinical practice. Strategies for optimizing assessment and aiding in identification and management of confounds that can limit diagnostic accuracy will be provided. Finally, clinical application of an interdisciplinary approach to identifying and managing confounds will be discussed and how assessment results can be used to identify trends in performance and guide prognostic counseling with families.
Magnetoencephalography-based approaches to epilepsy classification
Authors: Pan Ruoyao , Yang Chunlan , Li Zhimei , Ren Jiechuan , Duan Ying
Published: July 2023

Epilepsy is a chronic central nervous system disorder characterized by recurrent seizures. Not only does epilepsy severely affect the daily life of the patient, but the risk of premature death in patients with epilepsy is three times higher than that of the normal population. Magnetoencephalography (MEG) is a non-invasive, high temporal and spatial resolution electrophysiological data that provides a valid basis for epilepsy diagnosis, and used in clinical practice to locate epileptic foci in patients with epilepsy. It has been shown that MEG helps to identify MRI-negative epilepsy, contributes to clinical decision-making in recurrent seizures after previous epilepsy surgery, that interictal MEG can provide additional localization information than scalp EEG, and complete excision of the stimulation area defined by the MEG has prognostic significance for postoperative seizure control. However, due to the complexity of the MEG signal, it is often difficult to identify subtle but critical changes in MEG through visual inspection, opening up an important area of research for biomedical engineers to investigate and implement intelligent algorithms for epilepsy recognition. At the same time, the use of manual markers requires significant time and labor costs, necessitating the development and use of computer-aided diagnosis (CAD) systems that use classifiers to automatically identify abnormal activity. In this review, we discuss in detail the results of applying various different feature extraction methods on MEG signals with different classifiers for epilepsy detection, subtype determination, and laterality classification. Finally, we also briefly look at the prospects of using MEG for epilepsy-assisted localization (spike detection, high-frequency oscillation detection) due to the unique advantages of MEG for functional area localization in epilepsy, and discuss the limitation of current research status and suggestions for future research. Overall, it is hoped that our review will facilitate the reader to quickly gain a general understanding of the problem of MEG-based epilepsy classification and provide ideas and directions for subsequent research.
Similar additive effects of doxorubicin in combination with photon or proton irradiation in soft tissue sarcoma models
Authors: Bernardo Teresa , Behrends Carina , Klein Diana , Kuntze Anna , Timmermann Beate , von Neubeck Cläre
Published: July 2023

High-precision radiotherapy with proton beams is frequently used in the management of aggressive soft tissue sarcoma (STS) and is often combined with doxorubicin (Dox), the first-line chemotherapy for STS. However, current treatment approaches continue to result in high local recurrence rates often occurring within the treatment field. This strongly indicates the need of optimized treatment protocols taking the vast heterogeneity of STS into account, thereby fostering personalized treatment approaches. Here, we used preclinical STS models to investigate the radiation response following photon (X) or proton (H) irradiation alone and in combination with different treatment schedules of Dox. As preclinical models, fibrosarcoma (HT-1080), undifferentiated pleiomorphic sarcoma (GCT), and embryonal rhabdomyosarcoma (RD) cell lines were used; the latter two are mutated for TP53. The cellular response regarding clonogenic survival, apoptosis, cell-cycle distribution, proliferation, viability, morphology, and motility was investigated. The different STS cell types revealed a dose-dependent radiation response with reduced survival, proliferation, viability, and motility whereas G2/M phase arrest as well as apoptosis were induced. RD cells showed the most radiosensitive phenotype; the linear quadratic model fit could not be applied. In combined treatment schedules, Dox showed the highest efficiency when applied after or before and after radiation; Dox treatment only before radiation was less efficient. GCT cells were the most chemoresistant cell line in this study most probably due to their TP53 mutation status. Interestingly, similar additive effects could be observed for X or H irradiation in combination with Dox treatment. However, the additive effects were determined more frequently for X than for H irradiation. Thus, further investigations are needed to specify alternative drug therapies that display superior efficacy when combined with H therapy.
Adherence, belief, and knowledge about oral anticoagulants in patients with bioprosthetic heart valve replacement: a cross-sectional study
Authors: Ni Yun-Xia , Liu Lu-Lu , Feng Huang , Li Zhi , Qin Chao-Yi , Chen Miao
Published: July 2023

Aims: To investigate adherence to oral anticoagulants among patients after mechanical heart valve (BHV) replacement and further examine the mediating role of medication belief in the relationship between knowledge and medication adherence.

Background: The number of patients who undergo BHV replacement has increased in recent years. Short-term anticoagulant therapy is recommended for patients after BHV replacement. However, little is known about adherence to oral anticoagulant therapy and the underlying mechanisms among patients with BHV replacement.

Methods: A cross-sectional study was conducted between September 2022 and November 2022. A convenience sample of 323 patients who underwent BHV replacement was recruited from a tertiary public hospital in Southwest China. Data were collected by using the 8-item Morisky Medication Adherence Scale, Beliefs about Medicines Questionnaire-specific, and the Knowledge of Anticoagulation Questionnaire. The mediation model was tested by Hayes’s PROCESS macro. The STROBE checklist was used.

Results: Approximately 17.3% of participants had low adherence, 47.1% had medium adherence, and only 35.6% reported high adherence to oral anticoagulants. Knowledge and necessity beliefs were positively related to medication adherence, while concern beliefs were negatively correlated with medication adherence. Medication belief mediated the relationship between knowledge and adherence to oral anticoagulants.

Conclusion: Patients with BHV replacement demonstrated relatively low adherence to oral anticoagulant therapy. Efforts to enhance medication adherence should consider improving patients’ knowledge and medication beliefs.
Gain control in olfactory receptor neurons and the detection of temporal fluctuations in odor concentration
Authors: Tichy Harald , Hellwig Maria
Published: July 2023

The ability of the cockroach to locate an odor source in still air suggests that the temporal dynamic of odor concentration in the slowly expanding stationary plume alone is used to infer odor source distance and location. This contradicts with the well-established view that insects use the wind direction as the principle directional cue. This contribution highlights the evidence for, and likely functional relevance of, the capacity of the cockroach’s olfactory receptor neurons to detect and process—from one moment to the next—not only a succession of odor concentrations but also the rates at which concentration changes. This presents a challenge for the olfactory system because it must detect and encode the temporal concentration dynamic in a manner that simultaneously allows invariant odor recognition. The challenge is met by a parallel representation of odor identity and concentration changes in a dual pathway that starts from olfactory receptor neurons located in two morphologically distinct types of olfactory sensilla. Parallel processing uses two types of gain control that simultaneously allocate different weight to the instantaneous odor concentration and its rate of change. Robust gain control provides a stable sensitivity for the instantaneous concentration by filtering the information on fluctuations in the rate of change. Variable gain control, in turn, enhances sensitivity for the concentration rate according to variations in the duration of the fluctuation period. This efficiently represents the fluctuation of concentration changes in the environmental context in which such changes occur.
Impact of living and working in the heat on cognitive and psycho-physiological responses in outdoor fly-in fly-out tradesmen: a mining industry study
Authors: Taggart Sarah M. , Girard Olivier , Landers Grant J. , Ecker Ullrich K. H. , Wallman Karen E.
Published: July 2023

Objective: This study aimed to evaluate complex cognitive function, manual dexterity and psycho-physiological parameters in tradesmen working outdoors in the mining industry during summer and winter.

Methods: Twenty-six males working in a mining village in the north-west of Australia were assessed pre- and post-an 11-h shift at the start, middle, and end of a 14-day swing in summer (average daily temperature: 33.9°C, 38% RH; n = 12) and winter (24.3°C, 36% RH; n = 14).

Results: Working memory performance did not differ between seasons, over the swing or shift (p ≥ 0.053). Processing efficiency and manual dexterity performance did not differ between seasons (p ≥ 0.243), yet improved over the course of the swing (p ≤ 0.001) and shift (p ≤ 0.001). Core temperature, heart rate, thermal comfort, rating of perceived exertion and thermal sensation were not significantly different between seasons (p ≥ 0.076); however, average shift dehydration was greater in winter compared to summer (1.021 ± 0.005 vs. 1.018 ± 0.006; p = 0.014).

Conclusion: The ability to self-regulate the intensity of activity likely helped outdoor workers to thermoregulate effectively, minimising thermal strain during their swings and shifts, in turn explaining unaltered cognitive function and manual dexterity performance between seasons. Regardless of season, workers should receive education on dehydration and workplace risks to protect their health.
Cloning and expression characterization of elongation of very long-chain fatty acids protein 6 (elovl6) with dietary fatty acids, ambient salinity and starvation stress in Scylla paramamosain
Authors: Lin Zhideng , Wu Zhouyu , Huang Chaoyang , Lin Huangbin , Zhang Mingyao , Chen Mingfeng , Han Kunhuang , Huang Weiqing , Ruan Shaojiang
Published: July 2023

Introduction: Elongation of very long-chain fatty acids protein 6 (ELOVL6) played crucial roles in regulating energy expenditure and fatty acid metabolism. Many studies have performed to investigate the physiological roles and regulatory mechanisms of elovl6 in fish and animals, while few studies were reported in crustaceans.

Methods: Here we reported on the molecular cloning, tissue distribution and expression profiles in response to dietary fatty acids, ambient salinity and starvation stress in Scylla paramamosain by using rapid amplification of cDNA ends (RACE) and quantitative real-time PCR.

Results: Three elovl6 isoforms (named elovl6a, elovl6b and elovl6c) were isolated from S. paramamosain in the present study. The complete sequence of elovl6a was 1345 bp, the full-length sequence of elovl6b was 1419 bp, and the obtained elovl6c sequence was 1375 bp in full length. The elovl6a, elovl6b and elovl6c encoded 287, 329 and 301 amino acids respectively, and exhibited the typical structural features of ELOVL protein family members. Phylogenetic analysis showed that the ELOVL6a from S. paramamosain clustered most closely to ELOVL6 from Portunus trituberculatus and Eriocheir sinensis, while the ELOVL6b and ELOVL6c from S. paramamosain gathered alone into a single branch. Quantitative real-time PCR exhibited that the relatively abundant expression of elovl6b was observed in intestine and stomach, and the elovl6a and elovl6c were highly expressed in hepatopancreas. In addition, studies found that replacing fish oil with soybean oil could significantly increase the transcriptional levels of three elovl6 in hepatopancreas of S. paramamosain, and the expression of elovl6a and elovl6c in hepatopancreas were more sensitive to dietary fatty acids than the elovl6b. Compared with the normal sea water group (27‰), the expression of sterol-regulatory element binding protein1c (srebp-1), elovl6a, elovl6b and elovl6c were upregulated in the low salinity groups, particularly in 7‰. On the contrary, the starvation stress suppressed the expression of srebp-1, elovl6a, elovl6b and elovl6c.

Discussion: These results may contribute to understand the functions of elovl6 in fatty acid synthesis and regulatory mechanisms in crustaceans.
A BrLINE1-RUP insertion in BrCER2 alters cuticular wax biosynthesis in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Li Biyuan , Yue Zhichen , Ding Xiaoya , Zhao Yanting , Lei Juanli , Zang Yunxiang , Hu Qizan , Tao Peng
Published: July 2023

Glossiness is an important quality-related trait of Chinese cabbage, which is a leafy vegetable crop in the family Brassicaceae. The glossy trait is caused by abnormal cuticular wax accumulation. In this study, on the basis of a bulked segregant analysis coupled with next-generation sequencing (BSA-seq) and fine-mapping, the most likely candidate gene responsible for the glossy phenotype of Chinese cabbage was identified. It was subsequently named Brcer2 because it is homologous to AtCER2 (At4g24510). A bioinformatics analysis indicated a long interspersed nuclear element 1 (LINE-1) transposable element (named BrLINE1-RUP) was inserted into the first exon of Brcer2 in HN19-G via an insertion-mediated deletion mechanism, which introduced a premature termination codon. Gene expression analysis showed that the InDel mutation of BrCER2 reduced the transcriptional expression levels of Brcer2 in HN19-G. An analysis of cuticular waxes suggested that a loss-of-function mutation to BrCER2 in Chinese cabbage leads to a severe decrease in the abundance of very-long-chain-fatty-acids (> C28), resulting in the production of a cauline leaf, inflorescence stem, flower, and pistil with a glossy phenotype. These findings imply the insertion of the LINE-1 transposable element BrLINE1-RUP into BrCER2 can modulate the waxy traits of Chinese cabbage plants.
Interactively learning behavior trees from imperfect human demonstrations
Authors: Scherf Lisa , Schmidt Aljoscha , Pal Suman , Koert Dorothea
Published: July 2023

Introduction:In Interactive Task Learning (ITL), an agent learns a new task through natural interaction with a human instructor. Behavior Trees (BTs) offer a reactive, modular, and interpretable way of encoding task descriptions but have not yet been applied a lot in robotic ITL settings. Most existing approaches that learn a BT from human demonstrations require the user to specify each action step-by-step or do not allow for adapting a learned BT without the need to repeat the entire teaching process from scratch.

Method:We propose a new framework to directly learn a BT from only a few human task demonstrations recorded as RGB-D video streams. We automatically extract continuous pre- and post-conditions for BT action nodes from visual features and use a Backchaining approach to build a reactive BT. In a user study on how non-experts provide and vary demonstrations, we identify three common failure cases of an BT learned from potentially imperfect initial human demonstrations. We offer a way to interactively resolve these failure cases by refining the existing BT through interaction with a user over a web-interface. Specifically, failure cases or unknown states are detected automatically during the execution of a learned BT and the initial BT is adjusted or extended according to the provided user input.

Evaluation and results:We evaluate our approach on a robotic trash disposal task with 20 human participants and demonstrate that our method is capable of learning reactive BTs from only a few human demonstrations and interactively resolving possible failure cases at runtime.
Rethinking scientists’ ongoing participation in “feeding the world”
Authors: Haring Steven , Pesci Schmulevich Sasha , Manser Gwyneth M. , Cooper Mark H.
Published: July 2023

Agricultural science necessarily involves a commitment to some form of humanitarian and environmental ethics. For the past century, agricultural science has been based on a productivist ethic of maximizing agricultural production in pursuit or support of food security. Recognition of the ethical and political disposition of contemporary agricultural science can help scientists reorient their work to better address the urgent problems of the upcoming century: environmental degradation, climate change, and social inequality. A commitment to solving these problems is well within the scope of modern agricultural science. Supporting and furthering multidimensional food systems should replace productivism as an explicit goal of agricultural development and scientific innovation.
Research Progress on Porcine Reproductive and Respiratory Syndrome Virus NSP7 Protein
Authors: Li Huawei , Luo Qin , Jing Huiyuan , Song Yuzhen , Kong Weili , Zhao Mengmeng , Zhu Qingge
Published: July 2023

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and severe infectious disease caused by the PRRS virus (PRRSV). PRRS is characterized by reproductive disorders in sows and respiratory dysfunction in pigs. Non-structural protein 7 (NSP7) is one of the most conserved functional proteins in PRRSV, and it plays an important role in viral replication and humoral immune responses in infected hosts. This review discusses the biological characteristics of NSP7 to provide theoretical support for its application in PRRS diagnosis, novel vaccine design, and therapeutic drug development.
All Is Not Well: Contemporary Israeli Artistic Practices de-Assembling Dominant Narratives of Warfare and Water
Authors: Foresti Margherita
Published: July 2023

Well (2020) is an installation by Israeli artists Noga Or Yam and Faina Feigin. It investigates the story of an underground passage in Tel Aviv designed by a British Mandate-era Jewish architect. Starting from this building, the artists’ archival research leads them to the story of a water source which does not figure in the architect’s plan. While the story of the well is unearthed, so is one about the tense relations between the Jewish architect and the Palestinian orange merchant who inhabited the site before 1948. By restaging a hypothetical archive, Well reminds us of the problems inherent in narrative formation and erasure in the context of the Palestinian–Israeli conflict. Noga Or Yam also examined space and water in an earlier work, Black Soldier, White Soldier (2018): with the background sound of water drilling in southern Israel, urban photographic landscapes of Palestinian rooftops covered with water tanks are projected onto the walls. Water, either concealed or lacking, emerges in both works as a vehicle for unearthing a historical narrative that counters the official one. This research article reflects on contemporary art’s engagement with the formation of history, and how such engagement shapes the identity of present-day art in postcolonial realities.
Reinforcing the Effect of Warm Ocean Anomalies in the South China Sea on the Extended Tropical-Depression-Induced Heavy Rainfall Event in Hainan Island
Authors: Hao Sai , Chen Li , Liu Xiaoyan , Liu Kewei , Peng Wei
Published: July 2023

An unusually persistent and heavy rainfall event occurred in Hainan Island from 1 to 9 October 2010, in association with one extended tropical depression (TD) over the South China Sea (SCS). Based on rain-gauge precipitation, satellite altimetry, in situ Argo profile, air–sea enthalpy flux, and reanalysis data, this study investigates the impact of warm ocean anomalies in the SCS on the formation and intensification of the extended TD, and their reinforcing effect on TD-related heavy rainfall. The TD intensified and migrated northward to the vicinity of Hainan Island. A thicker-than-normal warm subsurface layer that was present beneath the positive sea surface temperature (SST) anomalies contained a sufficient upper-ocean heat content to effectively restrain the TD’s self-induced SST cooling effect, and available enthalpy fluxes were therefore sufficient to support the maintenance of the TD. The composite analyses confirm the reinforcing effect of warm oceanic anomalies in the central SCS off the south-central coast of Vietnam on heavy rainfall in Hainan Island, with the composite precipitation of “Warm eddy” cases being significantly larger in Hainan Island and northern Vietnam than that of the “Normal” cases, using reanalysis and remote sensing precipitation data over 29 years (1993–2021).
Methadone Potentiates the Cytotoxicity of Temozolomide by Impairing Calcium Homeostasis and Dysregulation of PARP in Glioblastoma Cells
Authors: Honc Ondrej , Novotny Jiri
Published: July 2023

Methadone is commonly used as an alternative to morphine in patients with pain associated with glioblastoma and other cancers. Although concomitant administration of methadone and cytostatics is relatively common, the effect of methadone on the efficacy of cytostatic drugs has not been well studied until recently. Moreover, the mechanism behind the effect of methadone on temozolomide efficacy has not been investigated in previous studies, or this effect has been automatically attributed to opioid receptors. Our findings indicate that methadone potentiates the effect of temozolomide on rat C6 glioblastoma cells and on human U251 and T98G glioblastoma cells and increases cell mortality by approximately 50% via a mechanism of action independent of opioid receptors. Our data suggest that methadone acts by affecting mitochondrial potential, the level of oxidative stress, intracellular Ca2+ concentration and possibly intracellular ATP levels. Significant effects were also observed on DNA integrity and on cleavage and expression of the DNA repair protein PARP-1. None of these effects were attributed to the activation of opioid receptors and Toll-like receptor 4. Our results provide an alternative perspective on the mechanism of action of methadone in combination with temozolomide and a potential strategy for the treatment of glioblastoma cell resistance to temozolomide.
Completion of Genetic Testing and Incidence of Pathogenic Germline Mutation among Patients with Early-Onset Colorectal Cancer: A Single Institution Analysis
Authors: Storandt Michael H. , Rogen Kara R. , Iyyangar Anushka , Schnell Rylie R. , Mitchell Jessica L. , Hubbard Joleen M. , Sinicrope Frank A. , Jatoi Aminah , Mahipal Amit , Shi Qian , Jin Zhaohui
Published: July 2023

Over the past 20 years, rates of early-onset colorectal cancer (eoCRC), defined as <50 years of age at diagnosis, have increased, with 16–25% associated with a pathogenic germline variant (PGV) resulting in a hereditary cancer syndrome. In the present study, we sought to further characterize PGVs observed in patients with eoCRC. We conducted a retrospective analysis of patients with a history of CRC referred for genetic counseling at Mayo Clinic Rochester between April 2019 and April 2022. Three hundred and three CRC patients were referred to medical genetics, including 124 with a history of eoCRC. Only 84 patients (68%) with eoCRC referred for genetic counseling completed genetic testing, with an average of 48 genes evaluated. PGVs were identified in 27.4% with eoCRC, including 8.3% with Lynch syndrome (LS). Other detected PGVs known to increase the risk of CRC included MUTYH (4.8%), CHEK2 (3.6%), APC, BMPR1A, and TP53 (1.3% each). Among those with aoCRC, 109 patients (61%) completed genetic testing, among which 88% had either a dMMR tumor, personal history of an additional LS malignancy, or family history of LS malignancy, with PGVs detected in 23% of patients. This study reinforces the importance for all patients with CRC, especially those with eoCRC, to undergo germline testing.