bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of Inflammatory Responses byCentella asiaticavia Suppression of IRAK1-TAK1 in Mouse Macrophages
Authors: Cho Young-Chang , Vuong Huong Lan , Ha Jain , Lee Sewoong , Park Jiyoung , Wibow Agung Eru , Cho Sayeon
Published: July 2020

Centella asiatica (L.) Urb. (C. asiatica) has been widely treated for inflammation-related diseases in China for thousands of years. While C. asiatica showed relevant effects as traditional medicine, the mechanism of C. asiatica suppressing inflammation has not been thoroughly investigated. Therefore, this study was conducted to reveal the anti-inflammatory mechanism of methanol fraction from C. asiatica (MCA) at the molecular level in murine macrophages. Levels of inflammation-related mediators were observed with treatment of MCA. MCA significantly suppressed nitric oxide production and iNOS expression in RAW 264.7 macrophages. Prostaglandin E2production was alleviated by MCA via the downregulation of cyclooxygenase-2. MCA treatment also reduced pro-inflammatory tumor necrosis factor-[Formula: see text] and interleukin (IL)-6 levels. LPS/D-GalN-induced acute hepatitis in mouse was alleviated by MCA treatment. In addition, MCA decreased the phosphorylation of inhibitory [Formula: see text]B[Formula: see text] (I[Formula: see text]B[Formula: see text]) at Ser32/36 and thereby blocked I[Formula: see text]B[Formula: see text] degradation. TXY motif phosphorylation in the activation loops of mitogen-activated protein kinases (MAPKs) was also suppressed by MCA treatment. Further investigation revealed that MCA inhibited transforming growth factor-[Formula: see text]-activated kinase 1 (TAK1) phosphorylation and IL-1 receptor-associated kinase (IRAK1) degradation, the upstream kinases activating nuclear factor [Formula: see text]B and MAPKs. Taken together, MCA exhibited anti-inflammatory properties via the downregulation of IRAK1-TAK1 signaling pathways.
Measurement and monitoring
Authors: Standing J. R. , Buss S. R.
Published: July 2020

Thematic collection:
This article is part of the Measurement and monitoring collection available at:
https://www.lyellcollection.org/cc/measurement-and-monitoring
Making Mice Mighty: recent advances in translational models of load-induced muscle hypertrophy
Authors: Murach Kevin A. , McCarthy John J. , Peterson Charlotte A. , Dungan Cory M.
Published: July 2020

The ability to genetically manipulate mice allows for gain- and loss-of-function in vivo, making them an ideal model for elucidating mechanisms of skeletal muscle mass regulation. Combining genetic models with mechanical muscle loading enables identification of specific factors involved in the hypertrophic response as well as the ability to test the requirement of those factors for adaptation, thereby informing performance and therapeutic interventions. Until recently, approaches for inducing mechanically mediated muscle hypertrophy (i.e., resistance-training analogs) have been limited and considered “nontranslatable” to humans. This mini-review outlines recent translational advances in loading-mediated strategies for inducing muscle hypertrophy in mice, and highlights the advantages and disadvantages of each method. The skeletal muscle field is poised for new breakthroughs in understanding mechanisms regulating load-induced muscle growth given the numerous murine tools that have very recently been described.
An Approach to the Computation of the Euler Number by means of the Vertex Chain Code
Authors: Bribiesca Ernesto , Braumann Ulf-Dietrich , Carrillo-Bermejo Angel , Sossa-Azuela Humberto
Published: July 2020

We present an approach to compute the number of holes in binary images using the Vertex Chain Code (VCC); the VCC was developed for representing and analyzing 2D shapes composed of cells. Using this code, it is possible to relate the outer to inner vertices of any 2D shape and to find interesting properties. Now, in this paper, we describe more properties of the VCC, such as the computation of the connected regions in a hole, the analysis of complementary chains, the computation of the number of holes in a binary shape or image, the computation of the Euler number, and the detection of convex and concave shapes. Finally, in order to illustrate the capabilities of proposed methods, we present the computation of topological properties of examples of objects of the real world.
Selected Hematological Biomarkers to Predict Acute Mortality in Emergency Department Patients. Recent Polish Hospital Statistics
Authors: Brzeźniakiewicz-Janus Katarzyna , Lancé Marcus Daniel , Tukiendorf Andrzej , Janus Tomasz , Franków Mirosław , Rupa-Matysek Joanna , Walkowiak Zuzanna , Gil Lidia
Published: July 2020

Background. Complete blood count (CBC), red cell distribution width (RDW), mean platelet volume (MPV), mean corpuscular volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), or platelet (PLT) count are referred as predictors of adverse clinical outcomes in patients. The aim of the research was to identify potential factors of acute mortality in Polish emergency department (ED) patients by using selected hematological biomarkers and routine statistical tools. Methods. The study presents statistical results on patients who were recently discharged from inpatient facilities within one month prior to the index ED visit. In total, the analysis comprised 14,881 patients with the first RDW, MPV, MCV, MCH, MCHC, or PLT biomarkers’ measurements recorded in the emergency department within the years 2016–2019 with a subsequent one month of all-cause mortality observation. The patients were classified with the codes of the International Statistical Classification of Diseases and Related Health Problems after 10th Revision (ICD10). Results. Based on the analysis of RDW, MPV, MCV, MCH, MCHC, and PLT on acute deaths in patients, we establish strong linear and quadratic relationships between the risk factors under study and the clinical response (P<0.05), however, with different mortality courses and threats. In our statistical analysis, (1) gradient linear relationships were found for RDW and MPV along an entire range of the analyzed biomarkers’ measurements, (2) following the quadratic modeling, an increasing risk of death above 95 fL was determined for MCV, and (3) no relation to excess death in ED patients was calculated for MCH, MCHC, and PLT. Conclusion. The study shows that there are likely relationships between blood counts and expected patient mortality at some time interval from measurements. Up to 1 month of observation since the first measurement of an hematological biomarker, RDW and MPV stand for a strong relationship with acute mortality of patients, whereas MCV, MCH, MCHC, and PLT give the U-shaped association, RDW and MPV can be established as the stronger predictors of early deaths of patients, MCV only in the highest levels (>95 fL), whereas MCH, MCHC, and PLT have no impact on the excess acute mortality in ED patients.
Pure Erythroid Leukemia in a Sickle Cell Patient Treated with Hydroxyurea
Authors: Yadav Dhiraj Kumar , Paul Thushara , Alhamar Mohamed , Inamdar Kedar , Guo Yue
Published: July 2020

We present a very rare case of pure erythroid leukemia arising in a young patient with sickle cell disease being treated with hydroxyurea for almost 5 years. Diagnosing and managing this rare condition has been a challenge and the majority of patients with pure erythroid leukemia have a very poor prognosis with survival in months despite treatment. This form of leukemia could be therapy related and in our case, hydroxyurea may have been responsible for the development of this aggressive condition.
Educacionalização do direito de punir
Authors: Medeiros Vítor Gonçalves Queiroz de
Published: July 2020

A partir do documento-base, da legislação pertinente e dos discursos de atores estatais, é possível listar três disjunções do SINASE em relação sistema das FEBEM’s: intersetorialização, interdisciplinarização e parametrização ética do atendimento. Sua soma resulta em uma educacionalização do direito de punir. Este artigo propõe uma análise deste processo em sua constituição e seus efeitos.
Prognostic Value of Cardiac Magnetic Resonance–Derived Right Ventricular Remodeling Parameters in Pulmonary Hypertension
Authors: Dong Yang , Pan Zhicheng , Wang Dongfei , Lv Jialan , Fang Juan , Xu Rui , Ding Jie , Cui Xiao , Xie Xudong , Wang Xingxiang , Chen, MD Yucheng , Guo Xiaogang
Published: July 2020

Background

Cardiac right ventricular remodeling plays a substantial role in pathogenesis, progression, and prognosis of pulmonary hypertension. Cardiac magnetic resonance is considered an excellent tool for evaluation of right ventricle. However, value of right ventricular remodeling parameters derived from cardiac magnetic resonance in predicting adverse events is controversial.

Methods

The Pubmed (MEDLINE), Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure platform (CNKI), China Science and Technology Journal Database (VIP), and Wanfang databases were systematically searched until November 2019. Studies reporting hazard ratios (HRs) for all-cause death and composite end point of pulmonary hypertension were included. Univariate HRs were extracted from the included studies to calculate pooled HRs of each right ventricular remodeling parameter.

Results

Eight studies with 1120 patients examining all-cause death (female: 44%–92%, age: 40–67 years old, follow-up time: 27–48 months) and 10 studies with 604 patients examining composite end point (female: 60%–83%, age: 29–57 years old, follow-up time: 10–68 months) met the criteria. Right ventricular ejection fraction was the only parameter which could predict both all-cause death (pooled HR=0.95;
P
=0.014) and composite end point (pooled HR=0.95;
P
<0.001), although right ventricular end-diastolic volume index (pooled HR=1.01;
P
<0.001), right ventricular end-systolic volume index (pooled HR=1.01,
P
=0.045), and right ventricular mass index (pooled HR=1.03,
P
=0.032) only predicted composite outcome. Similar results were observed when we conducted the meta-analysis among patients with World Health Organization type I of pulmonary hypertension.

Conclusions

Cardiac magnetic resonance–derived right ventricular remodeling parameters have independent prognostic value for all-cause death and composite end point of patients with pulmonary hypertension. Right ventricular ejection fraction was the strongest prognostic factor among all the right ventricular remodeling parameters. Right ventricular mass index, right ventricular end-diastolic volume index, and right ventricular end-systolic volume index also demonstrated prognostic value.
Texts, Voices and Tapes
Authors: Raia Annachiara
Published: July 2020

In this paper, I seek to investigate the manifold relationships between traditional and contemporary, oral and written Swahili poetry—in the utendi and mashairi forms—and its recitation in terms of the following considerations: how have advances in technology changed the production, transmission and reception of Swahili Islamic poetry? To what extent do writing and orality coexist in a recited text? What is the nature of performer identity formation within a “discourse network” of artists—the composer (mtungaji), reader (msomaji), and singer (mwimbaji)—who, in Goffman’s words, play “participation roles” and appropriate poetry belonging to other living poets or to their own (sometimes anonymous) ancestors? In an attempt to answer these questions, I provide examples of performers and their performative craft.
La cryptographie dans les manuscrits de la collection Lmūhūb Ūlaḥbīb
Authors: Mechehed Djamel Eddine
Published: July 2020

Nous présentons dans cet article les notes cryptographiques trouvées dans les manuscrits de la collection Lmūhūb Ūlaḥbīb, constituée vers le milieu du XIXème siècle à Ait Ūrṯilane en petite Kabylie (Algérie). Ce travail se fonde sur une analyse minutieuse de ces manuscrits d’ auteurs algériens et andalous de périodes diverses, comprises entre le XVème et le XIXème siècle.
Direct on-the-spot detection of SARS-CoV-2 in patients
Authors: Ben-Assa Nadav , Naddaf Rawi , Gefen Tal , Capucha Tal , Hajjo Haitham , Mandelbaum Noa , Elbaum Lilach , Rogov Peter , King Daniel A , Kaplan Shai , Rotem Assaf , Chowers Michal , Szwarcwort-Cohen Moran , Paul Mical , Geva-Zatorsky Naama
Published: July 2020

Many countries are currently in a state of lockdown due to the SARS-CoV-2 pandemic. One key requirement to safely transition out of lockdown is the continuous testing of the population to identify infected subjects. Currently, detection is performed at points of care using quantitative reverse-transcription PCR, thus requiring dedicated professionals and equipment. Here, we developed a protocol based on reverse transcribed loop-mediated isothermal amplification for the detection of SARS-CoV-2. This protocol is applied directly to SARS-CoV-2 nose and throat swabs, with no RNA purification step required. We tested this protocol on over 180 suspected patients, and compared the results to those obtained using the standard method. We further succeeded in applying the protocol to self-collected saliva samples from confirmed cases. Since the proposed protocol can detect SARS-CoV-2 from saliva and provides on-the-spot results, it allows simple and continuous surveillance of the community.

Impact statement

Humanity is currently experiencing a global pandemic with devastating implications on human health and the economy. Most countries are gradually exiting their lockdown state. We are currently lacking rapid and simple viral detections, especially methods that can be performed in the household. Here, we applied RT-LAMP directly on human clinical swabs and self-collected saliva samples. We adjusted the method to allow simple and rapid viral detection, with no RNA purification steps. By testing our method on over 180 human samples, we determined its sensitivity, and by applying it to other viruses, we determined its specificity. We believe this method has a promising potential to be applied world-wide as a simple and cheap surveillance test for SARS-CoV-2.
Probabilistic evaluation of maximum dynamic traffic load effects on cable-supported bridges under actual heavy traffic loads
Authors: Liu Yang , Wang Qinyong , Lu Naiwei
Published: July 2020

The traffic load has grown significantly in recent years, which might be a threat for the service safety of existing bridges. Thus, it is an urgent task to assess the actual traffic load effects on bridges, considering actual heavy traffic load instead of design traffic load. This study presents a framework for extrapolating maximum dynamic traffic load effects on large bridges using site-specific traffic monitoring data. The framework involves vehicle–bridge interaction analysis and probabilistic modelling of extreme values. The weigh-in-motion measurements of a busy highway in China were collected for stochastic traffic load modelling. Case studies of two long-span cable-supported bridge based on the weigh-in-motion measurements were conducted to demonstrate the effectiveness of the proposed framework. It is demonstrated that Rice’s level-crossing approach can capture both dynamic and probabilistic characteristics of the traffic load effects. The root-mean-square displacement of the cable-stayed bridge follows a C-type distribution, and the one for the suspension bridge follows an M-type distribution, which is associated with the first-order mode shapes of the two types of bridges. The amplification factors for the cable-stayed bridge and the suspension bridge are 5.9% and 3.6%, respectively. The numerical analysis indicates that the dynamic effect for extrapolation is weaker with the increase in bridge span length, but the effect of traffic volume growth will be more significant.
Ceramic 3D-Printed Titanium Cranioplasty
Authors: Mommaerts Maurice Y. , Depauw Paul R. , Nout Erik
Published: July 2020

Study Design:

Inlay cranioplasties following partial craniectomy in tumor or trauma cases and onlay cranioplasties for reconstructions of residual developmental skull anomalies are frequently performed using CAD-CAM techniques.

Objective:

In this case series, we present a novel cranial implant design, being a combination of 3D-printed titanium grade 23 and calcium phosphate paste (CeTi).

Methods:

The titanium patient-specific implant, manufactured using selective laser melting, has a latticed border with interconnected micropores. The cranioplasty is miniscrew fixed and its border zone subsequently partially filled with calcium phosphate paste to promote osteoinduction and osteoconduction. From April 2017 to April 2019, 8 patients have been treated with such a CeTi implant. The inlay cranioplasties were each time revision surgeries of complicated cases.

Results:

All implants were successful after a limited follow-up time (range 18-42 months). There were no dehiscences and no infections, and no complaints of thermal conduction.

Conclusions:

The proposed CeTi cranial implant combines the strength of titanium implants with the biological integration potential of ceramic implants and seems particularly resistant to infection, probably due to the biofunctionalized titanium surface and the antimicrobial activity of elevated intracellular free calcium levels.
Cestode strobilation: prediction of developmental genes and pathways
Authors: Paludo Gabriela Prado , Thompson Claudia Elizabeth , Miyamoto Kendi Nishino , Guedes Rafael Lucas Muniz , Zaha Arnaldo , de Vasconcelos Ana Tereza Ribeiro , Cancela Martin , Ferreira Henrique Bunselmeyer
Published: July 2020

Background

Cestoda is a class of endoparasitic worms in the flatworm phylum (Platyhelminthes). During the course of their evolution cestodes have evolved some interesting aspects, such as their increased reproductive capacity. In this sense, they have serial repetition of their reproductive organs in the adult stage, which is often associated with external segmentation in a developmental process called strobilation. However, the molecular basis of strobilation is poorly understood. To assess this issue, an evolutionary comparative study among strobilated and non-strobilated flatworm species was conducted to identify genes and proteins related to the strobilation process.

Results

We compared the genomic content of 10 parasitic platyhelminth species; five from cestode species, representing strobilated parasitic platyhelminths, and five from trematode species, representing non-strobilated parasitic platyhelminths. This dataset was used to identify 1813 genes with orthologues that are present in all cestode (strobilated) species, but absent from at least one trematode (non-strobilated) species. Development-related genes, along with genes of unknown function (UF), were then selected based on their transcriptional profiles, resulting in a total of 34 genes that were differentially expressed between the larval (pre-strobilation) and adult (strobilated) stages in at least one cestode species. These 34 genes were then assumed to be strobilation related; they included 12 encoding proteins of known function, with 6 related to the Wnt, TGF-β/BMP, or G-protein coupled receptor signaling pathways; and 22 encoding UF proteins. In order to assign function to at least some of the UF genes/proteins, a global gene co-expression analysis was performed for the cestode species Echinococcus multilocularis. This resulted in eight UF genes/proteins being predicted as related to developmental, reproductive, vesicle transport, or signaling processes.

Conclusions

Overall, the described in silico data provided evidence of the involvement of 34 genes/proteins and at least 3 developmental pathways in the cestode strobilation process. These results highlight on the molecular mechanisms and evolution of the cestode strobilation process, and point to several interesting proteins as potential developmental markers and/or targets for the development of novel antihelminthic drugs.
Molecular characterization of ocular dirofilariasis: a case report of Dirofilaria immitis in south-eastern Iran
Authors: Parsa Razieh , Sedighi Ali , Sharifi Iraj , Bamorovat Mehdi , Nasibi Saeid
Published: July 2020

Background

Dirofilariasis is a zoonotic parasitic infection transmitted from animals to humans by culicid mosquitoes. Although the disease can be caused by Dirofilaria spp. including Dirofilaria immitis and Dirofilaria repens, human ocular dirofilariasis due to D. immitis is relatively rare in the world. This study was aimed to present a case of ocular dirofilariasis caused by D. immitis in southeastern Iran.

Case presentation

A nematode extracted from the right eye of a 69-year-old man referred with clinical symptoms including itching and redness was examined. After the morphometric analysis, Dirofilaria parasite was detected. Afterwards, a piece of worm body was cut and DNA was extracted and a 680-bp gene fragment amplification and nucleotide sequencing were performed. Phylogenetic analysis revealed a D. immitis roundworm as the causative agent of infection. The patient was treated with antibiotics and corticosteroid and followed up for 1 month.

Conclusion

The present study provides the second report on ocular dirofilariasis caused by D. immitis isolated from a human in southeast Iran. Based on the available evidence, dirofilariasis in dogs has significantly increased in endemic areas such as Iran. Therefore, physicians should be aware of such zoonotic nematodes so as to take proper and timely action and treatment against the disease.
Polymorphisms of pfcrt, pfmdr1, and K13-propeller genes in imported falciparum malaria isolates from Africa in Guizhou province, China
Authors: She Danya , Wang Zhengyan , Liang Qiuguo , Lu Lidan , Huang Yuting , Zhang Ke , An Dong , Wu Jiahong
Published: July 2020

Background

Imported falciparum malaria from Africa has become a key public health challenge in Guizhou Province since 2012. Understanding the polymorphisms of molecular markers of drug resistance can guide selection of antimalarial drugs for the treatment of malaria. This study was aimed to analyze the polymorphisms of pfcrt, pfmdr1, and K13-propeller among imported falciparum malaria cases in Guizhou Province, China.

Method

Fifty-five imported falciparum malaria cases in Guizhou Province during 2012–2016 were included in this study. Their demographic information and filter paper blood samples were collected. Genomic DNA of Plasmodium falciparum was extracted from the blood samples, and polymorphisms of pfcrt, pfmdr1, and K13-propeller were analyzed with nested PCR amplification followed by sequencing. Data were analyzed with the SPSS17.0 software.

Results

The prevalence of pfcrt K76T, pfmdr1 N86Y, and pfmdr1 Y184F mutation was 56.6, 22.2, and 72.2%, respectively, in imported falciparum malaria cases in Guizhou Province. We detected two mutant haplotypes of pfcrt, IET and MNT, with IET being more commonly found (54.7%), and five mutant haplotypes of pfmdr1, of which NFD was the most frequent (53.7%). There were totally 10 combined haplotypes of pfcrt and pfmdr1, of which the haplotype IETNFD possessed a predominance of 28.8%. In addition, three nonsynonymous mutations (S459T, C469F, and V692L) and two synonymous mutations (R471R and V589V) were detected in K13-propeller, all having prevalence less than 6.0%. In particular, a candidate K13 resistance mutation, C469F, was identified for the first time from Democratic Republic of the Congo with the prevalence of 2.0%.

Conclusions

The high prevalence of IET haplotype of pfcrt and NFD haplotype of pfmdr1 suggests the presence of chloroquine, artemether/lumefantrine, and dihydroartemisinin/piperaquine resistance in these cases. Therefore cautions should be made to artemisinin therapy for P. falciparum in Africa. Continuous monitoring of anti-malarial drug efficacy in imported malaria cases is helpful for optimizing antimalarial drug therapy in Guizhou Province, China.
Serum levels of protein carbonyl, a marker of oxidative stress, are associated with overhydration, sarcopenia and mortality in hemodialysis patients
Authors: Song Young Rim , Kim Jwa-Kyung , Lee Hyung-Seok , Kim Sung Gyun , Choi Eun-Kyoung
Published: July 2020

Background

Increased oxidative stress in end-stage renal disease is regarded as one of the important mechanisms in the atherosclerosis and muscle wasting. However, studies examining the clinical significance of oxidative stress by direct measurement of these markers and its association with volume status and sarcopenia are limited.

Methods

A follow-up cross-sectional study was performed in stable hemodialysis (HD) patients and serum protein carbonyl levels were measured as a biomarker of oxidative stress. Additionally, multi-frequency body composition analysis, handgrip strength (HGS) and nutritional assessments were performed at baseline.

Results

Eighty-eight patients undergoing HD were included and 30 (34.1%) patients died during a mean follow-up of 5.2 years. The mean patient age was 60.6 ± 13.5 years, and the mean HD duration was 50.8 ± 41.3 months. In total, 16 patients (18.2%) were overhydrated, 49 (55.7%) had low HGS and 36 (40.9%) had low muscle mass. Serum protein carbonyl levels were associated with serum levels of albumin, prealbumin and transferrin, hydration status and low HGS. Overhydration (odds ratio [OR] 7.01, 95% CI 1.77–27.79,p = 0.006), prealbumin (OR 0.91, 95% CI 0.83–0.99,p = 0.030), subjective global assessment (OR 3.52, 95% CI 1.08–11.46,p = 0.037) and sarcopenia (OR 3.41, 95% CI 1.02–11.32,p = 0.046) were significantly related to increased serum protein carbonyl levels. Multivariate analysis showed that the serum levels of protein carbonyl (Hazard ratio [HR] 2.37, 95% CI 1.02–5.55,p = 0.036), albumin (HR 0.17, 95% CI 0.06–0.46,p = 0.003), prealbumin (HR 0.86, 95% CI 0.80–0.92,p = 0.001), overhydration (HR 2.31, 95% CI 1.26–8.71,p = 0.015) and sarcopenia (HR 2.72, 95% CI 1.11–6.63,p = 0.028) were independent determinants of all-cause mortality.

Conclusions

Serum protein carbonyl was significantly associated with overhydration, nutritional status and sarcopenia, and could be a new predictor of mortality in patients undergoing HD.
Transplantation of a kidney with a heterozygous mutation in the SLC22A12 (URAT1) gene causing renal hypouricemia: a case report
Authors: Tsuji Kiyokazu , Kitamura Mineaki , Muta Kumiko , Mochizuki Yasushi , Mori Takayasu , Sohara Eisei , Uchida Shinichi , Sakai Hideki , Mukae Hiroshi , Nishino Tomoya
Published: July 2020

Background

Renal hypouricemia (RHUC) is a genetic disorder caused by mutations in the SLC22A12 gene, which encodes the major uric acid (UA) transporter, URAT1. The clinical course of related, living donor-derived RHUC in patients undergoing kidney transplantation is poorly understood. Here, we report a case of kidney transplantation from a living relative who had an SLC22A12 mutation. After the transplantation, the recipient’s fractional excretion of UA (FEUA) decreased, and chimeric tubular epithelium was observed.

Case presentation

A 40-year-old man underwent kidney transplantation. His sister was the kidney donor. Three weeks after the transplantation, he had low serum-UA, 148.7 μmol/L, and elevated FEUA, 20.8% (normal: < 10%). The patient’s sister had low serum-UA (101.1 μmol/L) and high FEUA (15.8%) before transplant. Suspecting RHUC, we performed next-generation sequencing on a gene panel containing RHUC-associated genes. A heterozygous missense mutation in the SLC22A12 gene was detected in the donor, but not in the recipient. The recipient’s serum-UA level increased from 148.7 μmol/L to 231.9 μmol/L 3 months after transplantation and was 226.0 μmol/L 1 year after transplantation. His FEUA decreased from 20.8 to 11.7% 3 months after transplantation and was 12.4% 1 year after transplantation. Fluorescence in situ hybridization of allograft biopsies performed 3 months and 1 year after transplantation showed the presence of Y chromosomes in the tubular epithelial cells, suggesting the recipient’s elevated serum-UA levels were owing to a chimeric tubular epithelium.

Conclusions

We reported on a kidney transplant recipient that developed RHUC owing to his donor possessing a heterozygous mutation in the SLC22A12 (URAT1) gene. Despite this mutation, the clinical course was not problematic. Thus, the presence of donor-recipient chimerism in the tubular epithelium might positively affect the clinical course, at least in the short-term.
Multiple factors in the prediction of risk of recurrent vitreous haemorrhage after sutureless vitrectomy for non-clearing vitreous haemorrhage in patients with diabetic retinopathy
Authors: Ding Yuhua , Yao Bangtao , Hang Hui , Ye Hui
Published: July 2020

Background

We aimed to analyse multiple factors in the prediction of risk of postoperative recurrent vitreous haemorrhage (RVH) for non-clearing vitreous haemorrhage in patients with diabetic retinopathy (DR) who underwent sutureless vitrectomy with 23- (23G) or 25-gauge (25G) narrow-gauge systems.

Methods

A retrospective consecutive case series design was used. DR patients who underwent sutureless vitrectomy for non-clearing vitreous haemorrhage between June 2017 and October 2019 were enrolled. All operations were performed at a tertiary hospital. Patient demographics and risk factors, including age, gender, duration of diabetes, preoperative fasting blood sugar levels (FBSL), systolic blood pressure (SBP), serum creatinine (Cr), urea, triamcinolone acetonide (TA), electrical coagulation, air-fluid exchange, pan-retinal photocoagulation status (PRP), anti-vascular endothelial growth factor drug (anti-VEGF), and other factors, were recorded. Patients were divided into two groups based on the timing of their postoperative RVH: immediate postoperative RVH (within 2 weeks after operation) and delayed postoperative RVH (beyond 2 weeks after operation).

Results

Overall, 167 eyes (167patients) were enrolled. Seventy eyes were underwent 23G and 25G sutureless vitrectomy performed in 97 eyes, respectively. Postoperative RVH developed in 18 eyes (25.7%) in Group 23G and in 20 eyes (21.6%) in Group 25G (P = 0.540). Of these, 3 eyes (4.3%) had severed RVH in Group 23G compared with 5 eyes (5.2%) in Group 25G (P = 0.584). Delayed postoperative RVH occurred in 6 eyes (8.6%) in Group 23G and 8 eyes (8.2%) in Group 25G (P = 0.789). A binomial logistic regression analysis revealed that age, duration of diabetes, and Cr level were significantly associated with RVH in both Group 23G (P < 0.05) and Group 25G (P < 0.05).

Conclusions

The incidence and severity of RVH were 25.7 and 4.3%, respectively, in Group 23G and 21.6 and 5.2%, respectively, in Group 25G. Thus, the 23G sutureless vitrectomy approach was as safe as the 25G sutureless vitrectomy approach for treating vitreous haemorrhage in patients with DR. A younger age, shorter duration of diabetes, and higher Cr levels were risk factors for postoperative RVH in sutureless vitrectomy.
What influences practitioners’ readiness to deliver psychological interventions by telephone? A qualitative study of behaviour change using the Theoretical Domains Framework
Authors: Faija Cintia L. , Connell Janice , Welsh Charlotte , Ardern Kerry , Hopkin Elinor , Gellatly Judith , Rushton Kelly , Fraser Claire , Irvine Annie , Armitage Christopher J. , Wilson Paul , Bower Peter , Lovell Karina , Bee Penny
Published: July 2020

Background

Contemporary health policy is shifting towards remotely delivered care. A growing need to provide effective and accessible services, with maximal population reach has stimulated demand for flexible and efficient service models. The implementation of evidence-based practice has been slow, leaving many services ill equipped to respond to requests for non-face-to-face delivery. To address this translation gap, and provide empirically derived evidence to support large-scale practice change, our study aimed to explore practitioners’ perspectives of the factors that enhance the delivery of a NICE-recommended psychological intervention, i.e. guided self-help by telephone (GSH-T), in routine care. We used the Theoretical Domains Framework (TDF) to analyse our data, identify essential behaviour change processes and encourage the successful implementation of remote working in clinical practice.

Method

Thirty-four psychological wellbeing practitioners (PWPs) from the UK NHS Improving Access to Psychological Therapies (IAPT) services were interviewed. Data were first analysed inductively, with codes cross-matched deductively to the TDF.

Results

Analysis identified barriers to the delivery, engagement and implementation of GSH-T, within eight domains from the TDF: (i) Deficits in practitioner knowledge, (ii) Sub-optimal practitioner telephone skills, (iii) Practitioners’ lack of beliefs in telephone capabilities and self-confidence, (iv) Practitioners’ negative beliefs about consequences, (v) Negative emotions, (vi) Professional role expectations (vii) Negative social influences, and (viii) Challenges in the environmental context and resources. A degree of interdependence was observed between the TDF domains, such that improvements in one domain were often reported to confer secondary advantages in another.

Conclusions

Multiple TDF domains emerge as relevant to improve delivery of GSH-T; and these domains are theoretically and practically interlinked. A multicomponent approach is recommended to facilitate the shift from in-person to telephone-based service delivery models, and prompt behaviour change at practitioner, patient and service levels. At a minimum, the development of practitioners’ telephone skills, an increase in clients’ awareness of telephone-based treatment, dilution of negative preconceptions about telephone treatment, and robust service level guidance and standards for implementation are required. This is the first study that provides clear direction on how to improve telephone delivery and optimise implementation, aligning with current mental health policy and service improvement.
Belgian psychiatrists’ attitudes towards, and readiness to engage in, euthanasia assessment procedures with adults with psychiatric conditions: a survey
Authors: Verhofstadt Monica , Audenaert Kurt , Van den Broeck Kris , Deliens Luc , Mortier Freddy , Titeca Koen , Pardon Koen , Chambaere Kenneth
Published: July 2020

Background

Although the Belgian assessment pathway for legal euthanasia requires the engagement of at least one psychiatrist, little is known about psychiatrists’ attitudes towards euthanasia for adults with psychiatric conditions (APC). This study aims to gauge psychiatrists’ attitudes towards and readiness to engage in euthanasia assessment and/or performance procedures in APC.

Methods

This cross-sectional survey study was performed between November 2018 and April 2019. The survey was sent to a sample of 499 eligible psychiatrists affiliated to the Flemish Association for Psychiatry, a professional association that aims to unite and represent all psychiatrists working in Flanders, the Dutch-speaking, northern part of Belgium. The Association’s members comprise an estimated 80–90% of all psychiatrists active in Flanders. Only psychiatrists working with APC (83% of the association’s total membership) were included. Factorial Anova and Chi Square tests were performed to examine if and to what extent psychiatrists’ backgrounds were associated with, respectively, their attitudes and their readiness to play a role in euthanasia procedures concerning APC.

Results

One hundred eighty-four psychiatrists completed the questionnaire (response rate 40.2%); 74.5% agree that euthanasia should remain permissible for APC. However, 68.9% question some of the approaches taken by other physicians during the euthanasia assessment and only half consider euthanasia assessment procedures compatible with the psychiatric care relationship. Where active engagement is concerned, an informal referral (68%) or preliminary advisory role (43.8%) is preferred to a formal role as a legally required advising physician (30.3%), let alone as performing physician (< 10%).

Conclusion

Although three quarters agree with maintaining the legal option of euthanasia for APC, their readiness to take a formal role in euthanasia procedures appears to be limited. More insight is required into the barriers preventing engagement and what psychiatrists need, be it education or clarification of the legal requirements, to ensure that patients can have their euthanasia requests assessed adequately.
Soil nematode community and crop productivity in response to 5-year biochar and manure addition to yellow cinnamon soil
Authors: Liu Xiaodan , Zhang Dengxiao , Li Huixin , Qi Xiuxiu , Gao Ya , Zhang Yibo , Han Yanlai , Jiang Ying , Li Hui
Published: July 2020

Background

Manure and biochar soil amendments have shown many benefits to soil quality and crop productivity. This study aimed to reveal the effects of biochar and manure applications on soil fertility improvement and crop productivity in yellow cinnamon soil.

Results

This study based on a 5-year field experiment. Four treatments were designed, included the control (CK), biochar amendment, manure amendment, and both biochar and manure amendment (BM). The results showed that: after five years, both biochar and manure treatment improved soil structure by increasing soil mean weight diameter (MWD), and soil water and nutrient supply was also increased by increasing the contents of water content, available potassium and available phosphorus. The productivity was also enhanced as wheat yield under the biochar, manure, and BM treatments increased by 3.59–11.32% compared with CK. In addition, biochar and manure treatment increased soil microbial biomass carbon (MBC) by > 15%, and soil total nematode abundance was significantly increased. Furthermore, the nematode community structure was significantly affected by biochar and manure treatment, dominant trophic group in CK was herbivores, but bacterivores were dominant in the biochar and manure treatments. The distribution of nematode genera was closely related to soil chemical properties and microbial biomass. Increases in the Shannon's diversity index, and decreases in the dominance index and summed maturity index after the 5-year treatment indicated a sustainable soil ecosystem after the biochar and manure applications.

Conclusions

These findings indicate that biochar and manure result in better soil quality and increased productivity in yellow cinnamon soil.
Differential loss of effector genes in three recently expanded pandemic clonal lineages of the rice blast fungus
Authors: Latorre Sergio M. , Reyes-Avila C. Sarai , Malmgren Angus , Win Joe , Kamoun Sophien , Burbano Hernán A.
Published: July 2020

Background

Understanding the mechanisms and timescales of plant pathogen outbreaks requires a detailed genome-scale analysis of their population history. The fungus Magnaporthe (Syn. Pyricularia) oryzae—the causal agent of blast disease of cereals— is among the most destructive plant pathogens to world agriculture and a major threat to the production of rice, wheat, and other cereals. Although M. oryzae is a multihost pathogen that infects more than 50 species of cereals and grasses, all rice-infecting isolates belong to a single genetically defined lineage. Here, we combined the two largest genomic datasets to reconstruct the genetic history of the rice-infecting lineage of M. oryzae based on 131 isolates from 21 countries.

Results

The global population of the rice blast fungus consists mainly of three well-defined genetic groups and a diverse set of individuals. Multiple population genetic tests revealed that the rice-infecting lineage of the blast fungus probably originated from a recombining diverse group in Southeast Asia followed by three independent clonal expansions that took place over the last ~ 200 years. Patterns of allele sharing identified a subpopulation from the recombining diverse group that introgressed with one of the clonal lineages before its global expansion. Remarkably, the four genetic lineages of the rice blast fungus vary in the number and patterns of presence and absence of candidate effector genes. These genes encode secreted proteins that modulate plant defense and allow pathogen colonization. In particular, clonal lineages carry a reduced repertoire of effector genes compared with the diverse group, and specific combinations of presence and absence of effector genes define each of the pandemic clonal lineages.

Conclusions

Our analyses reconstruct the genetic history of the rice-infecting lineage of M. oryzae revealing three clonal lineages associated with rice blast pandemics. Each of these lineages displays a specific pattern of presence and absence of effector genes that may have shaped their adaptation to the rice host and their evolutionary history.
Effects of reconstruction techniques after proximal gastrectomy: a systematic review and meta-analysis
Authors: Shaibu Zakari , Chen Zhihong , Mzee Said Abdulrahman Salim , Theophilus Acquah , Danbala Isah Adamu
Published: July 2020

Background

Additional studies comparing several reconstruction methods after proximal gastrectomy have been published; of note, it is necessary to update systematic reviews and meta-analysis from the current evidence-based literature.

Aim

To expand the current knowledge on feasibility and safety, and also to analyze postoperative outcomes of several reconstructive techniques after proximal gastrectomy.

Methods

PubMed, Google Scholar, and Medline databases were searched for original studies, and relevant literature published between the years 1966 and 2019 concerning various reconstructive techniques on proximal gastrectomy were selected. The postoperative outcomes and complications of the reconstructive techniques were assessed. Meta-analyses were performed using Rev-Man 5.0. A total of 29 studies investigating postoperative outcomes of double tract reconstruction, jejunal pouch interposition, jejunal interposition, esophagogastrostomy, and double flap reconstruction were finally selected in the quantitative analysis.

Result

Pooled incidences of reflux esophagitis for double tract reconstruction, jejunal pouch interposition, jejunal interposition esophagogastrostomy, and double flap reconstruction were 8.6%, 13.8%, 13.8%, 19.3%, and 8.9% respectively. Meta-analysis showed a decreased length of hospital in the JI group as compared to the JPI group (heterogeneity: Chi2 = 1.34, df = 1 (P = 0.25); I2 = 26%, test for overall effect: Z = 2.22 (P = 0.03). There was also a significant difference between JI and EG in length of hospital stay with heterogeneity: Chi2 = 1.40, df = 3 (P = 0.71); I2 = 0%, test for overall effect: Z = 5.04 (P < 0.00001). Operative time was less in the EG group as compared to the JI group (heterogeneity: Chi2 = 31.09, df = 5 (P < 0.00001); I2 = 84%, test for overall effect: Z = 32.35 (P < 0.00001).

Conclusion

Although current reconstructive techniques present excellent anti-reflux efficacy, the optimal reconstructive method remains to be determined. The double flap reconstruction proved to lower the rate of complication, but the DTR, JI, JPI, and EG groups showed higher incidence of complications in anastomotic leakage, anastomotic stricture, and residual food. In the meta-analysis result, the complications between the JI, JPI, and EG were comparable but the EG group showed to have better postoperative outcomes concerning the operative time, blood loss, and length of hospital stay.
Feasibility of totally laparoscopic pylorus-preserving gastrectomy with intracorporeal gastro-gastrostomy for early gastric cancer: a retrospective cohort study
Authors: Akiyama Yuji , Sasaki Akira , Iwaya Takeshi , Fujisawa Ryosuke , Sasaki Noriyuki , Nikai Haruka , Endo Fumitaka , Baba Shigeaki , Hasegawa Yasushi , Kimura Toshimoto , Takahara Takeshi , Nitta Hiroyuki , Otsuka Koki , Koeda Keisuke
Published: July 2020

Background

Pylorus-preserving gastrectomy (PPG) has been accepted as a function-preserving surgery for the treatment of early gastric cancer in East Asian countries. Therefore, this study aimed to evaluate the feasibility and safety of totally laparoscopic PPG (TLPPG) with intracorporeal anastomosis.

Methods

A total of 43 patients with early gastric cancer underwent laparoscopy-assisted PPG (LAPPG) with extracorporeal anastomosis between May 2006 and November 2012. The operative outcomes of 22 patients who underwent TLPPG between November 2012 and February 2019 were evaluated, and data were compared with that of the LAPPG group.

Results

No significant difference in the operative time was observed between the two groups. Blood loss was lower in the TLPPG group (18.5 mL) than in the LAPPG group (30.7 mL, p = 0.008), and the length of abdominal incision was shorter in the TLPPG group (3.8 cm) than in the LAPPG group (4.7 cm, p < 0.001). No significant difference in the complication rate was observed between the two groups (13.6% in the TLPPG vs. 9.3% in the LAPPG group, p = 0.594). No anastomosis-related complications occurred in either group. No significant between-group difference was observed in the delayed gastric emptying (TLPPG, 9.1 vs. LAPPG, 7%, p = 0.762). The initiation of postoperative fluid (TLPPG, 1.0 day vs. LAPPG, 3.0 days, p < 0.001) and meal (TLPPG, 3.0 days vs. LAPPG, 4.0 days, p < 0.001) intake was earlier in the TLPPG group than in the LAPPG group. No significant between-group difference was observed in the postoperative hospital stay.

Conclusions

The findings of this study suggest that TLPPG with intracorporeal reconstruction not only is as feasible and safe as LAPPG for the treatment of patients with early gastric cancer but also provides certain advantages such as reduced blood loss and wound size.
YYFZBJS ameliorates colorectal cancer progression in ApcMin/+ mice by remodeling gut microbiota and inhibiting regulatory T-cell generation
Authors: Sui Hua , Zhang Lu , Gu Kaijuan , Chai Ni , Ji Qing , Zhou Lihong , Wang Yan , Ren Junze , Yang Limei , Zhang Bimeng , Hu Jing , Li Qi
Published: July 2020

Background

Progression of Colorectal cancer (CRC) is influenced by single or compounded environmental factors. Accumulating evidence shows that microbiota can influence the outcome of cancer immunotherapy. T cell, one of the main populations of effector immune cells in antitumor immunity, has been considered as a double-edged sword during the progression of CRC. Our previous studies indicate that traditional Chinese herbs (TCM) have potential anticancer effects in improving quality of life and therapeutic effect. However, little is known about the mechanism of TCM formula in cancer prevention.

Methods

Here, we used C57BL/6 JApcMin/+mice, an animal model of human intestinal tumorigenesis, to investigate the gut bacterial diversity and their mechanisms of action in gastrointestinal adenomas, and to evaluate the effects of Yi-Yi-Fu-Zi-Bai-Jiang-San (YYFZBJS) on of colon carcinogenesis in vivo and in vitro. Through human-into-mice fecal microbiota transplantation (FMT) experiments from YYFZBJS volunteers or control donors, we were able to differentially modulate the tumor microbiome and affect tumor growth as well as tumor immune infiltration.

Results

We report herein, YYFZBJS treatment blocked tumor initiation and progression inApcMin/+mice with less change of body weight and increased immune function. Moreover, diversity analysis of fecal samples demonstrated that YYFZBJS regulated animal’s natural gut flora, includingBacteroides fragilis,Lachnospiraceaeand so on. Intestinal tumors from conventional and germ-free mice fed with stool from YYFZBJS volunteers had been decreased. Some inflammation’ expression also have been regulated by the gut microbiota mediated immune cells. Intestinal lymphatic, and mesenteric lymph nodes (MLN), accumulated CD4+ CD25+ Foxp3 positive Treg cells were reduced by YYFZBJS treatment inApcMin/+mice. Although YYFZBJS had no inhibition on CRC cell proliferation by itself, the altered Tregs mediated by YYFZBJS repressed CRC cancer cell growth, along with reduction of the phosphorylation of β-catenin.

Conclusions

In conclusion, we demonstrated that gut microbiota and Treg were involved in CRC development and progression, and we propose YYFZBJS as a new potential drug option for the treatment of CRC.

Graphical abstract