bluuebunny/crossref_metadata_2025 huggingface dataset:
Presence of the Extended-Spectrum-β-Lactamase and Plasmid-Mediated AmpC-Encoding Genes in Escherichia coli from Companion Animals—A Study from a University-Based Veterinary Hospital in Taipei, Taiwan
Authors: Liu Fang-Ling , Kuan Nan-Ling , Yeh Kuang-Sheng
Published: December 2021

Extended-spectrum-β-lactamase (ESBL) and AmpC β-lactamase are two enzymes commonly found in Enterobacteriaceae that confer resistance to major antibiotics, such as third-generation cephalosporins that are widely prescribed for both human and animals. We screened for Escherichia coli producing ESBL and plasmid-mediated AmpC β-lactamase (pAmpC) from dogs and cats brought to National Taiwan University Veterinary Hospital, Taipei, Taiwan from 29 June 2020, to 31 December 2020. The genotypes and phylogenetic relatedness of these E. coli were also analyzed. Fifty samples of E. coli obtained from 249 bacterial isolates were included in this study. Among them, eight isolates had ESBL, seven had pAmpC, and one had both. Thirty-two percent (16/50) of E. coli isolates were resistant to third-generation cephalosporins. The detected ESBL genes included the blaCTX-M-1 and blaCTX-M-9 groups, and the blaCMY-2 group was the only gene type found in pAmpC. ESBL-producing E. coli belonged to the pathogenic phylogroup B2, and the sequence types (STs) were ST131 and ST1193. Three isolates were determined to be ST131-O25b, a highly virulent epidemic clone. The pAmpC-producing E. coli were distributed in multiple phylogroups, primarily the commensal phylogroup B1. The STs of the pAmpC-producing E. coli included ST155, ST315, ST617, ST457, ST767, ST372, and ST93; all of these have been reported in humans and animals. Imipenem was active against all the ESBL/pAmpC-producing E. coli; however, since in humans it is a last-resort antimicrobial, its use in companion animals should be restricted.
Antioxidants: Looking Forward after a Decade
Authors: Omaye Stanley T.
Published: December 2021

As our journal, Antioxidants, celebrates its tenth year, I want to express my gratitude to our publisher, MDPI, the editorial staff, our editors and reviewers, and the many authors for making it possible for Antioxidants to become a respective premier journal [...]
Field Tests on Bearing Characteristics of Large-Diameter Combined Tip-and-Side Post Grouted Drilled Shafts
Authors: Zhang Zhitong , Gong Weiming , Dai Guoliang , Cao Xiaolin , Zhu Yu , Huang Hao
Published: December 2021

This paper presents a field study on the axial behavior of four large-diameter drilled shafts embedded in coarse sand. The grouting and loading test procedures were reported. The bearing capacity of shafts (TS1 and TS2) and grouted drilled shafts (TS3 and TS4) were herein determined by the bi-directional static test and top-down load test, respectively. The enhancement mechanism of bearing characteristics of the grouted shafts was discussed in detail. The test results indicate that the bearing characteristics and load transfer mechanisms of the test shafts were significantly affected by the quantity of pressurized cement slurry and the mechanical properties of the soil surrounding the shafts. Furthermore, the tip resistance of shaft can be mobilized more rapidly and fully after grouting, the side and tip resistance are mobilized in a more synchronized and coordinated manner due to the pre-mobilization of the grouted cement. Additionally, the standard penetration test (SPT) prediction model was introduced to calculate and predict the SPT blow counts of soil after grouting. The results show that the post grouting has a more obvious improvement on the strength of cohesionless soil.
Postural Instability after Stepping on a Stair in Older Adults: A Pilot Study
Authors: Lee Hyokeun , Byun Kyungseok
Published: December 2021

This study aimed to examine how older adults (OA) control their postural stability after stepping on a stair in comparison to young adults (YA). Ten OA and 10 YA participated in this study. Participants ascended a single stair (15 cm high by 30 cm wide) which was secured atop one of the force plates. Ground reaction forces (GRFs) and center of pressure (COP) motion data were obtained from the force plate under the stair. After standing on the stair with both feet, GRFs and COP data for a 3 s duration were analyzed to assess postural variables, including time to stabilization (TTS), COP velocity (COPVEL), and COP sway area (COPSWAY). A significant difference in TTS in the anterior–posterior direction between OA and YA (p = 0.032) was observed, indicating that OA had difficulty stabilizing their body posture after the stair ascent compared to YA. For COP postural variables, no significant differences in COPVEL (p = 0.455) and COPSWAY (p = 0.176) were observed between OA and YA. Study findings indicate that older adults have less capacity to regain postural stability compared to young adults following a challenging dynamic movement.
Simulation of the Marangoni Effect and Phase Change Using the Particle Finite Element Method
Authors: Bobach Billy-Joe , Boman Romain , Celentano Diego , Terrapon Vincent E. , Ponthot Jean-Philippe
Published: December 2021

A simulation method is developed herein based on the particle finite element method (PFEM) to simulate processes with surface tension and phase change. These effects are important in the simulation of industrial applications, such as welding and additive manufacturing, where concentrated heat sources melt a portion of the material in a localized fashion. The aim of the study is to use this method to simulate such processes at the meso-scale and thereby gain a better understanding of the physics involved. The advantage of PFEM lies in its Lagrangian description, allowing for automatic tracking of interfaces and free boundaries, as well as its robustness and flexibility in dealing with multiphysics problems. A series of test cases is presented to validate the simulation method for these two effects in combination with temperature-driven convective flows in 2D. The PFEM-based method is shown to handle both purely convective flows and those with the Marangoni effect or melting well. Following exhaustive validation using solutions reported in the literature, the obtained results show that an overall satisfactory simulation of the complex physics is achieved. Further steps to improve the results and move towards the simulation of actual welding and additive manufacturing examples are pointed out.
Analysis of Improvement of BIM-Based Digitalization in Engineering, Procurement, and Construction (EPC) Projects in China
Authors: Zheng Yu , Tang Llewellyn C. M. , Chau K. W.
Published: December 2021

Digitalization is considered a dynamic change process powered by the rapid development of innovative concepts (e.g., building information modeling) that brings substantial potential benefits to the construction industry. However, previous studies of the benefits of digitalization (BIM) were mainly based on laboratory data rather than actual practices, which compromised the reliability of the results for construction practitioners. This study investigated the impact of digitalization in EPC construction projects by integrating descriptive statistics and survey-based exploratory factor analysis (SEFA). After a detailed review of related studies and meetings with experts, ten main variables and 14 unified variables were identified. The data on these variables were collected by questionnaire surveys and the extraction of information from project documentation. A total of 62 participants from 33 EPC construction projects responded to the questionnaire survey. The SEFA results indicated that digitalization can improve both the cost and time performance, with greater potential for improvement in time performance for EPC projects. In a scenario with limited digitalization implementation, this research could motivate future researchers to develop more applied research and guidelines to achieve best practices in the domain of the digitalization of construction projects.
Adsorptive Recovery of Cu2+ from Aqueous Solution by Polyethylene Terephthalate Nanofibres Modified with 2-(Aminomethyl)Pyridine
Authors: Totito Thandiwe Crystal , Laatikainen Katri , Pereao Omoniyi , Bode-Aluko Chris , Petrik Leslie
Published: December 2021

The accumulation of plastic waste products in the environment has adversely affected wildlife and human beings. Common plastics that accumulate in the environment are plastics that are made of polyethylene terephthalate (PET) polymer. PET plastic waste products can be recycled for beneficial use, which would reduce their negative impacts. In this study, modified PET or waste PET (WPET) from plastic bottles was blended with powder commercial 2-(aminomethyl)pyridine (SiAMPy) resin and electrospun into composite nanofibres and applied for Cu2+ adsorption. PET-SiAMPy or WPET-SiAMPy composite nanofibres fibre diameters from the HRSEM images were 90–140 nm and 110–155 nm, respectively. In batch adsorption experiments, PET-SiAMPy or WPET-SiAMPy composite nanofibres achieved Cu2+ adsorption equilibrium within 60 secs of contact time with 0.98 mmol/g (89.87%) or 1.24 mmol/g (96.04%) Cu2+ adsorption capacity. The Cu2+ complex formation rate (k) with WPET-SiAMPy was 0.0888 with the mole ratio of Cu2+ and WPET-SiAMPy nanofibres 1:2. The complex molecular formula formed was Cu(WPET-SiAMPy)2 with a square planar geometry structure. The WPET-SiAMPy nanofibres’ adsorption was best fitted to the Freundlich isotherm. WPET-SiAMPy composite nanofibres were considered highly efficient for Cu2+ adsorption from aqueous solution and could be regenerated at least five times using 5 M H2SO4.
Effect Mechanism of Connection Joints in Fabricated Station Structures
Authors: He Huafei , Li Zhaoping
Published: December 2021

The seismic response of a fabricated subway station is a complex structural connection problem that depends on the mechanical properties of the joints. In order to obtain the optimal joint distribution of a fabricated station structure under earthquake action, three finite element models of a single ring structure of fabricated subway stations assembled with seven, five, and four prefabricated components were proposed. Seismic wave characteristics, peak acceleration, and coupled horizontal and vertical seismic components were considered to study the seismic response of the fabricated subway station structure with different forms of the joint distribution. The dynamic time history method was used to analyze the seismic response in three aspects: structure plastic strain, interlayer relative deformation, and internal force. The damage indexes and residual strength indexes of the joints were offered based on the concrete damage index to evaluate the joints’ damage degree. The results showed that the joints of the vault or bottom plate had little influence on the seismic response of the fabricated station structure. The sidewall joints had the obvious seismic response and the most severe damage under horizontal ground motion or coupled ground motion, which were the weak joints of the fabricated station structure. The existence of vertical ground motion aggravated the damage degree of sidewall joints, making the damage occurrence time of sidewall joints earlier and the damage end time extended. On the premise of meeting the mechanical load and site requirements, an assembly scheme with fewer prefabricated components can be selected.
Greenhouse Gas Impact of Algal Bio-Crude Production for a Range of CO2 Supply Scenarios
Authors: Arora Pratham , Chance Ronald R. , Hendrix Howard , Realff Matthew J. , Thomas Valerie M. , Yuan Yanhui
Published: December 2021

Refined bio-crude production from hydrothermal liquefaction of algae holds the potential to replace fossil-based conventional liquid fuels. The microalgae act as natural carbon sequestrators by consuming CO2. However, this absorbed CO2 is released to the atmosphere during the combustion of the bio-crude. Thus, the life-cycle greenhouse gas (GHG) emissions of refined bio-crude are linked to the production and supply of the materials involved and the process energy demands. One prominent raw material is CO2, which is the main source of carbon for algae and the subsequent products. The emissions associated with the supply of CO2 can have a considerable impact on the sustainability of the algae-based refined bio-crude production process. Furthermore, the diurnal algae growth cycle complicates the CO2 supply scenarios. Traditionally, studies have relied on CO2 supplied from existing power plants. However, there is potential for building natural gas or biomass-based power plants with the primary aim of supplying CO2 to the biorefinery. Alternately, a direct air capture (DAC) process can extract CO2 directly from the air. The life-cycle GHG emissions associated with the production of refined bio-crude through hydrothermal liquefaction of algae are presented in this study. Different CO2 supply scenarios, including existing fossil fuel power plants and purpose-built CO2 sources, are compared. The integration of the CO2 sources with the algal biorefinery is also presented. The CO2 supply from biomass-based power plants has the highest potential for GHG reduction, with a GHG footprint of −57 g CO2 eq./MJ refined bio-crude. The CO2 supply from the DAC process has a GHG footprint of 49 CO2 eq./MJ refined bio-crude, which is very similar to the scenario that considers the supply of CO2 from an existing conventional natural gas-based plant and takes credit for the carbon utilization.
Boriding of Laser-Clad Inconel 718 Coatings for Enhanced Wear Resistance
Authors: Lindner Thomas , Günen Ali , Töberling Gerd , Vogt Sabrina , Karakas Mustafa Serdar , Löbel Martin , Lampke Thomas
Published: December 2021

Nickel-based superalloys are particularly suitable for applications under corrosive conditions. Economic advantages can be achieved by limiting the use of materials to the surface region. Furthermore, the tribological property profile can be significantly improved by surface hardening. In the present study, the possibility of a process combination comprising a coating and a surface hardening technology was investigated. For this purpose, Inconel 718 coatings were applied to austenitic stainless steel by laser cladding. Subsequently, a thermochemical surface hardening by boriding was carried out. Scanning electron microscopic (SEM) examinations were performed to evaluate the microstructure. The phase composition was determined by means of X-ray diffraction (XRD) for the different states of the coating system. The influence of thermochemical hardening was investigated for different wear conditions. The increase in microhardness and wear resistance clearly demonstrates the utilization potential of the presented process combination.
Producing Synthetic Dataset for Human Fall Detection in AR/VR Environments
Authors: Zherdev Denis , Zherdeva Larisa , Agapov Sergey , Sapozhnikov Anton , Nikonorov Artem , Chaplygin Sergej
Published: December 2021

Human poses and the behaviour estimation for different activities in (virtual reality/augmented reality) VR/AR could have numerous beneficial applications. Human fall monitoring is especially important for elderly people and for non-typical activities with VR/AR applications. There are a lot of different approaches to improving the fidelity of fall monitoring systems through the use of novel sensors and deep learning architectures; however, there is still a lack of detail and diverse datasets for training deep learning fall detectors using monocular images. The issues with synthetic data generation based on digital human simulation were implemented and examined using the Unreal Engine. The proposed pipeline provides automatic “playback” of various scenarios for digital human behaviour simulation, and the result of a proposed modular pipeline for synthetic data generation of digital human interaction with the 3D environments is demonstrated in this paper. We used the generated synthetic data to train the Mask R-CNN-based segmentation of the falling person interaction area. It is shown that, by training the model with simulation data, it is possible to recognize a falling person with an accuracy of 97.6% and classify the type of person’s interaction impact. The proposed approach also allows for covering a variety of scenarios that can have a positive effect at a deep learning training stage in other human action estimation tasks in an VR/AR environment.
A Numerical Analysis of the Influence of Nozzle Geometric Structure on Spontaneous Steam Condensation and Irreversibility in the Steam Ejector Nozzle
Authors: Li He , Wang Xiaodong , Huang Hailong , Ning Jiuxin , Tu Jiyuan
Published: December 2021

The spontaneous condensation of wet steam often occurs in the steam ejector nozzle, this deteriorates the performance of the steam ejector. In this paper, we take changing the geometric parameters of the nozzle as the focus of our research and construct an internal connection between steam’s condensation behavior and the nozzle’s throat radius, the nozzle’s divergent section expansion angle, and the nozzle’s divergent section length. Our numerical simulation results indicate that an increase in the throat diameter and reduction of the divergent section’s expansion angle can inhibit steam condensation behavior, to a certain extent. In particular, the steam condensation behavior will disappear at a 0° expansion angle, but it is not affected by the change in the divergent section’s length. In addition, the irreversibility that is seen under different changes to the nozzle’s structure parameters was investigated and the results show that the entropy generation that is caused by a phase change accounts for a much higher proportion of the total entropy generation than heat transport and viscous dissipation do. This indicates that steam’s condensation behavior makes a large amount of irreversible energy, resulting in energy waste and reducing the performance of the nozzle. Therefore, this study can provide a theoretical reference for suppressing the spontaneous condensation behavior of steam by changing the nozzle’s geometry.
Characteristics and Long-Term Variability of Occurrences of Storm Surges in the Baltic Sea
Authors: Wolski Tomasz , Wiśniewski Bernard
Published: December 2021

Understanding the characteristics of storm surges is especially important in the context of ongoing climate changes, which often lead to catastrophic events in the coastal zones of seas and oceans. For this reason, this paper presents the characteristics of the Baltic Sea storm surges and trends in their occurrences through the past 60 years. The study material was based on hourly sea level readings, spanning the years 1961–2020, retrieved from 45 Baltic Sea tide gauges, as well as air pressure and wind field data. Owing to the analysis and visualization of storm situations, two main types of storm surges were identified and characterized: a surge driven by wind and a surge driven by subpressure associated with an active low pressure area. This paper also discusses a third, mixed type of storm surge. Further analyses have indicated that through the past 60 years in the Baltic Sea, the duration of high sea level has increased by 1/3, the average number of storm surges has increased from 3.1 to 5.5 per year, and the maximum annual sea levels have increased—with a trend value of 0.28 cm/year. These processes, also observed in other marine basins, provide strong evidence for contemporary climate change.
Astroclimatic Conditions at the Hoa Lac and Nha Trang Astronomical Observatories
Authors: Shikhovtsev Artem Y. , Kovadlo Pavel G. , Kopylov Evgeniy A. , Ibrahimov Mansur A. , Le Xuan Huy
Published: December 2021

The paper presents the first results of astroclimatic studies at the sites of the Hoa Lac and Nha Trang astronomical observatories. Our study employs Era-5 data covering a 10-yr time period (2011–2020). An analysis of the main astroclimatic characteristic, namely, the wind speed in the upper layers of the atmosphere, was performed. We calculated space distributions of the wind speed averaged in the height bin from 100 to 200 hPa. Using hourly data on pressure levels we analyzed probability distributions of the wind speed at high-level maxima at the sites of the observatories. At the Nha Trang observatory the period with a potentially high astroclimatic conditions falls on the spring when high recurrence of weak winds is observed. At the Hoa Lac observatory the best conditions are observed in the summer and the autumn. In this period, the median wind speeds are low. Additionally, we calculated spectra of the air temperature using the Fast Fourier Transform. We analyzed the deformations of the spectra with heights in a wide range of scales. At the site of the Nha Trang Astronomical Observatory, the amplitude of daily air temperature variations in the surface layer is approximately 1.5–2.5 times smaller compared to the Hoa Lac Observatory. We showed that the low-frequency maximum in the spectra is pronounced only in the lower layers of the atmosphere.
Dynamic of Particulate Matter for Quotidian Aerosol Sources in Indoor Air
Authors: Mostafa Mostafa Yuness Abdelfatah , Khalaf Hyam Nazmy Bader , Zhukovsky Michael V.
Published: December 2021

A correlation between the mass concentration of particulate matter (PM) and the occurrence of health-related problems or diseases has been confirmed by several studies. However, little is known about indoor PM concentrations, their associated risks or their impact on health. In this work, the PM1, PM2.5 and PM10 produced by different indoor aerosol sources (candles, cooking, electronic cigarettes, tobacco cigarettes, mosquito coils and incense) are studied. The purpose is to quantify the emission characteristics of different indoor particle sources. The mass concentration, the numerical concentration, and the size distribution of PM from various sources were determined in an examination room 65 m3 in volume. Sub-micrometer particles and approximations of PM1, PM2.5 and PM10 concentrations were measured simultaneously using a diffusion aerosol spectrometer (DAS). The ultrafine particle concentration for the studied indoor aerosol sources was approximately 7 × 104 particles/cm3 (incense, mosquito coils and electronic cigarettes), 1.2 × 105 particles/cm3 for candles and cooking and 2.7 × 105 particles/cm3 for tobacco cigarettes. The results indicate that electronic cigarettes can raise indoor PM2.5 levels more than 100 times. PM1 concentrations can be nearly 55 and 30 times higher than the background level during electronic cigarette usage and tobacco cigarette burning, respectively. It is necessary to study the evaluation of indoor PM, assess the toxic potential of internal molecules and develop and test strategies to ensure the improvement of indoor air quality.
One-Dimensional Disordered Bosonic Systems
Authors: D’Errico Chiara , Tarallo Marco G.
Published: December 2021

Disorder is everywhere in nature and it has a fundamental impact on the behavior of many quantum systems. The presence of a small amount of disorder, in fact, can dramatically change the coherence and transport properties of a system. Despite the growing interest in this topic, a complete understanding of the issue is still missing. An open question, for example, is the description of the interplay of disorder and interactions, which has been predicted to give rise to exotic states of matter such as quantum glasses or many-body localization. In this review, we will present an overview of experimental observations with disordered quantum gases, focused on one-dimensional bosons, and we will connect them with theoretical predictions.
Study of Positron Impact Scattering from Methane and Silane Using an Analytically Obtained Static Potential with Correlation Polarization
Authors: Mahato Dibyendu , Sharma Lalita , Srivastava Rajesh
Published: December 2021

A detailed study of positron impact elastic scattering from methane and silane is carried out using a model potential consisting of static and polarization potentials. The static potential for the molecular target is obtained analytically by using accurate Gaussian molecular wavefunctions. The molecular orbitals are expressed as a linear combination of Gaussian atomic orbitals. Along with the analytically obtained static potential, a correlation polarization potential is also added to construct the model potential. Utilizing the model potential, the Schrödinger equation is solved using the partial wave phase shift analysis method, and the scattering amplitude is obtained in terms of the phase shifts. Thereafter, the differential, integrated and total cross sections are calculated. These cross-section results are compared with the previously reported measurements and theoretical calculations.
Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte
Authors: Yang Huixia , Kolben Thomas , Meister Sarah , Paul Corinna , van Dorp Julia , Eren Sibel , Kuhn Christina , Rahmeh Martina , Mahner Sven , Jeschke Udo , von Schönfeldt Viktoria
Published: December 2021

In vitro maturation (IVM) of oocytes is a promising assisted reproductive technology (ART) deemed as a simple and safe procedure. It is mainly used in patients with impaired oocyte maturation and in fertility preservation for women facing the risk of losing fertility. However, to date, it is still not widely used in clinical practice because of its underperformance. The influencing factors, such as biphasic IVM system, culture medium, and the supplementation, have a marked effect on the outcomes of oocyte IVM. However, the role of different culture media, supplements, and follicular priming regimens in oocyte IVM have yet to be fully clarified and deserve further investigation.
Tissue Engineering of the Urethra: From Bench to Bedside
Authors: Pastorek Dusan , Culenova Martina , Csobonyeiova Maria , Skuciova Veronika , Danisovic Lubos , Ziaran Stanislav
Published: December 2021

Tissue engineering (TE) is a promising approach for repair/substitution of damaged tissues and organs. Urethral strictures are common and serious health conditions that impair quality of life and may lead to serious organ damage. The search for ideal materials for urethral repair has led to interest of scientists and surgeons in urethral TE. Over the last decades, a significant amount of preclinical studies and considerable progress have been observed. In contrast, urethral TE has made slow progress in clinical practice so far. To address this, we conducted a systematic review of the literature on clinical applications of TE constructs for urethral repair in the last three decades. In summary, the TE approach is promising and effective, but many issues remain that need to be addressed for broader adoption of TE in urethral repair. Better design of trials, better cooperation of research groups and centralization could lead to reduction of costs and slowly proceed to commercialization and routine use of TE products for urethral reconstruction.
High-Resolution Episcopic Microscopy (HREM) in Multimodal Imaging Approaches
Authors: Keuenhof Katharina , Kavirayani Anoop , Reier Susanne , Geyer Stefan , Weninger Wolfgang , Walter Andreas
Published: December 2021

High-resolution episcopic microscopy (HREM) is a three-dimensional (3D) episcopic imaging modality based on the acquisition of two-dimensional (2D) images from the cut surface of a block of tissue embedded in resin. Such images, acquired serially through the entire length/depth of the tissue block, are aligned and stacked for 3D reconstruction. HREM has proven to be specifically advantageous when integrated in correlative multimodal imaging (CMI) pipelines. CMI creates a composite and zoomable view of exactly the same specimen and region of interest by (sequentially) correlating two or more modalities. CMI combines complementary modalities to gain holistic structural, functional, and chemical information of the entire sample and place molecular details into their overall spatiotemporal multiscale context. HREM has an advantage over in vivo 3D imaging techniques on account of better histomorphologic resolution while simultaneously providing volume data. HREM also has certain advantages over ex vivo light microscopy modalities. The latter can provide better cellular resolution but usually covers a limited area or volume of tissue, with limited 3D structural context. HREM has predominantly filled a niche in the phenotyping of embryos and characterisation of anatomic developmental abnormalities in various species. Under the umbrella of CMI, when combined with histopathology in a mutually complementary manner, HREM could find wider application in additional nonclinical and translational areas. HREM, being a modified histology technique, could also be incorporated into specialised preclinical pathology workflows. This review will highlight HREM as a versatile imaging platform in CMI approaches and present its benefits and limitations.
Systematic Review with Meta-Analysis: Diagnostic Accuracy of Pro-C3 for Hepatic Fibrosis in Patients with Non-Alcoholic Fatty Liver Disease
Authors: Mak Anne Linde , Lee Jenny , van Dijk Anne-Marieke , Vali Yasaman , Aithal Guruprasad P. , Schattenberg Jörn M. , Anstee Quentin M. , Brosnan M. Julia , Zafarmand Mohammad Hadi , Ramsoekh Dewkoemar , Harrison Stephen A. , Nieuwdorp Max , Bossuyt Patrick M. , Holleboom Adriaan G.
Published: December 2021

The prevalence and severity of non-alcoholic fatty liver disease (NAFLD) is increasing, yet adequately validated tests for care paths are limited and non-invasive markers of disease progression are urgently needed. The aim of this work was to summarize the performance of Pro-C3, a biomarker of active fibrogenesis, in detecting significant fibrosis (F ≥ 2), advanced fibrosis (F ≥ 3), cirrhosis (F4) and non-alcoholic steatohepatitis (NASH) in patients with NAFLD. A sensitive search of five databases was performed in July 2021. Studies reporting Pro-C3 measurements and liver histology in adults with NAFLD without co-existing liver diseases were eligible. Meta-analysis was conducted by applying a bivariate random effects model to produce summary estimates of Pro-C3 accuracy. From 35 evaluated reports, eight studies met our inclusion criteria; 1568 patients were included in our meta-analysis of significant fibrosis and 2058 in that of advanced fibrosis. The area under the summary curve was 0.81 (95% CI 0.77–0.84) in detecting significant fibrosis and 0.79 (95% CI 0.73–0.82) for advanced fibrosis. Our results support Pro-C3 as an important candidate biomarker for non-invasive assessment of liver fibrosis in NAFLD. Further direct comparisons with currently recommended non-invasive tests will demonstrate whether Pro-C3 panels can outperform these tests, and improve care paths for patients with NAFLD.
HDAC6 Inhibition Extinguishes Autophagy in Cancer: Recent Insights
Authors: Passaro Eugenia , Papulino Chiara , Chianese Ugo , Toraldo Antonella , Congi Raffaella , Del Gaudio Nunzio , Nicoletti Maria Maddalena , Benedetti Rosaria , Altucci Lucia
Published: December 2021

Autophagy is an essential intracellular catabolic mechanism involved in the degradation and recycling of damaged organelles regulating cellular homeostasis and energy metabolism. Its activation enhances cellular tolerance to various stresses and is known to be involved in drug resistance. In cancer, autophagy has a dual role in either promoting or blocking tumorigenesis, and recent studies indicate that epigenetic regulation is involved in its mechanism of action in this context. Specifically, the ubiquitin-binding histone deacetylase (HDAC) enzyme HDAC6 is known to be an important player in modulating autophagy. Epigenetic modulators, such as HDAC inhibitors, mediate this process in different ways and are already undergoing clinical trials. In this review, we describe current knowledge on the role of epigenetic modifications, particularly HDAC-mediated modifications, in controlling autophagy in cancer. We focus on the controversy surrounding their ability to promote or block tumor progression and explore the impact of HDAC6 inhibitors on autophagy modulation in cancer. In light of the fact that targeted drug therapy for cancer patients is attracting ever increasing interest within the research community and in society at large, we discuss the possibility of using HDAC6 inhibitors as adjuvants and/or in combination with conventional treatments to overcome autophagy-related mechanisms of resistance.
Does Preoperative Acute Pancreatitis Inevitably Delay Pancreatoduodenectomy in Patients with Periampullary Tumors?
Authors: Yoon So Jeong , Lee Okjoo , Jung Ji Hye , Shin Sang Hyun , Heo Jin Seok , Han In Woong
Published: December 2021

Preoperative acute pancreatitis (PAP) in patients with periampullary tumor can cause technical difficulties when performing pancreatoduodenectomy (PD) but perioperative risks of PAP remain unclear. The purpose of this study was to investigate the impact of PAP on surgical outcomes and determine the optimal timing of PD. Patients undergoing surgery for periampullary tumors between 2009 and 2018 were included. Simple random sampling (1:4) was performed to compare outcomes between the PAP group and the control group. Operative failure was defined as exploration-only or unwanted total pancreatectomy. The rate of operative failure was higher in the PAP group than in the control group (6.6% vs. 0%, p < 0.001). There was no significant difference in postoperative outcomes including complications or in-hospital mortality between the two groups. Surgical outcomes were compared after dividing PAP groups by intervals (2, 3, or 4 weeks) between the onset of PAP and surgery, and there were no differences between the groups. In conclusion, in spite of the increased risk of operation failure, PD could be performed in PAP patients at comparable rates of postoperative complications. Further study is needed to select patients with PAP in proper conditions for performing PD.
Safety and Efficacy of Peptide-Receptor Radionuclide Therapy in Elderly Neuroendocrine Tumor Patients
Authors: Theiler Deborah , Cattaneo Marco , Dierickx Lawrence O. , Igaz Peter , Grozinsky-Glasberg Simona , Bournaud Claire , O’Dorisio Thomas , O’Dorisio M. Sue , Wild Damian , Christ Emanuel , Nicolas Guillaume P.
Published: December 2021

Peptide receptor radionuclide therapy (PRRT) is a well-established treatment in somatostatin receptor-expressing neuroendocrine tumours (NETs). The safety and efficacy of PRRT in >79 years old patients (EP) have not been systematically investigated. All patients with inoperable/metastatic/progressive G1/G2 NET, >79 years (EP), treated with PRRT at the University Hospital of Basel between 2006 and 2018, were enrolled in this retrospective matched cohort study. Each patient was manually matched with ≥1 younger patient (YP = 60–70 years). The primary endpoint was toxicity. Toxicity (subacute, long-term) was graded according to the criteria for adverse events (CTCAE) v5.0. All toxicity grades ≥ 3, or whose delta (Δ) to baseline were ≥2, were considered significant. The odds ratio (OR) for developing toxicity was tested for non-inferiority of EP vs. YP. Clinical response to PRRT and overall survival (OS) were assessed as secondary outcome measures. Forty-eight EP and 68 YP were enrolled. Both cohorts were balanced regarding median time since diagnosis, tumour location, grading, treatment scheme, and baseline biochemical parameters, except for eGFR (EP: 61 ± 16 vs. YP: 78 ± 19; mL/min/1.73 m2). Twenty-two grade ≥ 3 or Δ ≥ 2 subacute hematotoxicities occurred in 10 EP (10.3% of cycles) and 37 in 19 YP (11.6% of cycles; p = NS). Long-term grade ≥ 3 renal toxicity occurred in 7 EP and 2 YP (p = NS). The median OS was 3.4 years (EP) vs. 6.0 years (YP), HR: 1.50 [0.75, 2.98], p = NS. PRRT is a valid therapeutic option in elderly NET patients with similar toxicity and non-inferior survival compared to matched younger patients.
Genome-Wide DNA Methylation Signatures Predict the Early Asymptomatic Doxorubicin-Induced Cardiotoxicity in Breast Cancer
Authors: Bauer Michael , Todorova Valentina , Stone Annjanette , Carter Weleetka , Plotkin Matthew , Hsu Ping-Ching , Wei Jeanne , Su Joseph , Makhoul Issam
Published: December 2021

Chemotherapy with doxorubicin (DOX) may cause unpredictable cardiotoxicity. This study aimed to determine whether the methylation signature of peripheral blood mononuclear cells (PBMCs) prior to and after the first cycle of DOX-based chemotherapy could predict the risk of cardiotoxicity in breast cancer patients. Cardiotoxicity was defined as a decrease in left ventricular ejection fraction (LVEF) by >10%. DNA methylation of PBMCs from 9 patients with abnormal LVEF and 10 patients with normal LVEF were examined using Infinium HumanMethylation450 BeadChip. We have identified 14,883 differentially methylated CpGs at baseline and 18,718 CpGs after the first cycle of chemotherapy, which significantly correlated with LVEF status. Significant differentially methylated regions (DMRs) were found in the promoter and the gene body of SLFN12, IRF6 and RNF39 in patients with abnormal LVEF. The pathway analysis found enrichment for regulation of transcription, mRNA splicing, pathways in cancer and ErbB2/4 signaling. The preliminary results from this study showed that the DNA methylation profile of PBMCs may predict the risk of DOX-induced cardiotoxicity prior to chemotherapy. Further studies with larger cohorts of patients are needed to confirm these findings.
Cerium-, Europium- and Erbium-Modified ZnO and ZrO2 for Photocatalytic Water Treatment Applications: A Review
Authors: Gaggero Elisa , Calza Paola , Cerrato Erik , Paganini Maria Cristina
Published: December 2021

In the last decades photocatalysis has become one of the most employed technologies for the implementation of the so-called Advanced Oxidation Processes (AOPs) for the removal of harmful pollutants from wastewaters. The materials identified as the best photocatalysts are transition metal oxides, in which the band structure allows charge carrier separation upon solar irradiation. The photoinduced charge carrier can thus cause oxidative and reductive redox reactions at the surface, inducing the formation of the radical species able to initiate the AOPs. Despite the great advantages of this process (non-toxic, cheap and environmentally clean), the main drawback lies in the fact that the most efficient semiconductors are only able to absorb UV irradiation, which accounts for only 5% of the total solar irradiation at the Earth’s surface and not enough to generate the required amount of electron-hole pairs. On the other hand, many efforts have been devoted to the sensitization of wide band gap transition metal oxides to visible light, which represents a higher percentage (almost 45%) in the solar electromagnetic spectrum. Among all the strategies to sensitize transition metal oxides to visible irradiation, doping with lanthanides has been less explored. In this regard, lanthanides offer a unique electronic configuration, consisting in 4f orbitals shielded by a 5s5p external shell. This occurrence, coupled with the different occupation of the localized 4f orbitals would provide an astounding opportunity to tune these materials’ properties. In this review we will focus in depth on the modification of two promising photocatalytic transition metal oxides, namely ZnO and ZrO2, with cerium, europium and erbium atoms. The aim of the work is to provide a comprehensive overview of the influence of lanthanides on the structural, optical and electronic properties of the modified materials, emphasizing the effect of the different 4f orbital occupation in the three considered doping atoms. Moreover, a large portion of the discussion will be devoted to the structural-properties relationships evidencing the improved light absorption working mechanism of each system and the resulting enhanced photocatalytic performance in the abatement of contaminants in aqueous environments.
Nanostructured Fe-N-C as Bifunctional Catalysts for Oxygen Reduction and Hydrogen Evolution
Authors: da Silva Freitas Williane , Machado Pico Pedro Pablo , D’Epifanio Alessandra , Mecheri Barbara
Published: December 2021

The development of electrocatalysts for energy conversion and storage devices is of paramount importance to promote sustainable development. Among the different families of materials, catalysts based on transition metals supported on a nitrogen-containing carbon matrix have been found to be effective catalysts toward oxygen reduction reaction (ORR) and hydrogen evolution reaction (HER) with high potential to replace conventional precious metal-based catalysts. In this work, we developed a facile synthesis strategy to obtain a Fe-N-C bifunctional ORR/HER catalysts, involving wet impregnation and pyrolysis steps. Iron (II) acetate and imidazole were used as iron and nitrogen sources, respectively, and functionalized carbon black pearls were used as conductive support. The bifunctional performance of the Fe-N-C catalyst toward ORR and HER was investigated by cyclic voltammetry, rotating ring disk electrode experiments, and electrochemical impedance spectroscopy in alkaline environment. ORR onset potential and half-wave potential were 0.95 V and 0.86 V, respectively, indicating a competitive performance in comparison with the commercial platinum-based catalyst. In addition, Fe-N-C had also a good HER activity, with an overpotential of 478 mV @10 mAcm−2 and Tafel slope of 133 mVdec−1, demonstrating its activity as bifunctional catalyst in energy conversion and storage devices, such as alkaline microbial fuel cell and microbial electrolysis cells.
Recent Progress in the Steam Reforming of Bio-Oil for Hydrogen Production: A Review of Operating Parameters, Catalytic Systems and Technological Innovations
Authors: Pafili Anastasia , Charisiou Nikolaos , Douvartzides Savvas , Siakavelas Georgios , Wang Wen , Liu Guanqing , Papadakis Vagelis , Goula Maria
Published: December 2021

The present review focuses on the production of renewable hydrogen through the catalytic steam reforming of bio-oil, the liquid product of the fast pyrolysis of biomass. Although in theory the process is capable of producing high yields of hydrogen, in practice, certain technological issues require radical improvements before its commercialization. Herein, we illustrate the fundamental knowledge behind the technology of the steam reforming of bio-oil and critically discuss the major factors influencing the reforming process such as the feedstock composition, the reactor design, the reaction temperature and pressure, the steam to carbon ratio and the hour space velocity. We also emphasize the latest research for the best suited reforming catalysts among the specific groups of noble metal, transition metal, bimetallic and perovskite type catalysts. The effect of the catalyst preparation method and the technological obstacle of catalytic deactivation due to coke deposition, metal sintering, metal oxidation and sulfur poisoning are addressed. Finally, various novel modified steam reforming techniques which are under development are discussed, such as the in-line two-stage pyrolysis and steam reforming, the sorption enhanced steam reforming (SESR) and the chemical looping steam reforming (CLSR). Moreover, we argue that while the majority of research studies examine hydrogen generation using different model compounds, much work must be done to optimally treat the raw or aqueous bio-oil mixtures for efficient practical use. Moreover, further research is also required on the reaction mechanisms and kinetics of the process, as these have not yet been fully understood.
Cytosolic Self-DNA—A Potential Source of Chronic Inflammation in Aging
Authors: Akbari Mansour , Shanley Daryl P. , Bohr Vilhelm A. , Rasmussen Lene Juel
Published: December 2021

Aging is the consequence of a lifelong accumulation of stochastic damage to tissues and cellular components. Advancing age closely associates with elevated markers of innate immunity and low-grade chronic inflammation, probably reflecting steady increasing incidents of cellular and tissue damage over the life course. The DNA sensing cGAS-STING signaling pathway is activated by misplaced cytosolic self-DNA, which then initiates the innate immune responses. Here, we hypothesize that the stochastic release of various forms of DNA from the nucleus and mitochondria, e.g., because of DNA damage, altered nucleus integrity, and mitochondrial damage, can result in chronic activation of inflammatory responses that characterize the aging process. This cytosolic self-DNA-innate immunity axis may perturb tissue homeostasis and function that characterizes human aging and age-associated pathology. Proper techniques and experimental models are available to investigate this axis to develop therapeutic interventions.
Activation of Downstream mTORC1 Target Ribosomal Protein S6 Kinase (S6K) Can Be Found in a Subgroup of Dutch Patients with Granulomatous Pulmonary Disease
Authors: Kraaijvanger Raisa , Seldenrijk Kees , Beijer Els , Damen Jan , Wilson Jayne Louise , Weichhart Thomas , Grutters Jan C. , Veltkamp Marcel
Published: December 2021

Mechanistic target of rapamycin complex 1 (mTORC1) has been linked to different diseases. The mTORC1 signaling pathway is suggested to play a role in the granuloma formation of sarcoidosis. Recent studies demonstrated conflicting data on mTORC1 activation in patients with sarcoidosis by measuring activation of its downstream target S6 kinase (S6K) with either 33% or 100% of patients. Therefore, the aim of our study was to reevaluate the percentage of S6K activation in sarcoidosis patients in a Dutch cohort. To investigate whether this activation is specific for sarcoid granulomas, we also included Dutch patients with other granulomatous diseases of the lung. The activation of the S6K signaling pathway was evaluated by immunohistochemical staining of its downstream effector phospho-S6 in tissue sections. Active S6K signaling was detected in 32 (43%) of the sarcoidosis patients. Twelve (31%) of the patients with another granulomatous disorder also showed activated S6K signaling, demonstrating that the mTORC1 pathway may be activated in a range for different granulomatous diseases (p = 0.628). Activation of S6K can only be found in a subgroup of patients with sarcoidosis, as well as in patients with other granulomatous pulmonary diseases, such as hypersensitivity pneumonitis or vasculitis. No association between different clinical phenotypes and S6K activation can be found in sarcoidosis.
Flexible Impedimetric Electronic Nose for High-Accurate Determination of Individual Volatile Organic Compounds by Tuning the Graphene Sensitive Properties
Authors: Lu Tianqi , Al-Hamry Ammar , Rosolen José Mauricio , Hu Zheng , Hao Junfeng , Wang Yuchao , Adiraju Anurag , Yu Tengfei , Matsubara Elaine Yoshiko , Kanoun Olfa
Published: December 2021

We investigated functionalized graphene materials to create highly sensitive sensors for volatile organic compounds (VOCs) such as formaldehyde, methanol, ethanol, acetone, and isopropanol. First, we prepared VOC-sensitive films consisting of mechanically exfoliated graphene (eG) and chemical graphene oxide (GO), which have different concentrations of structural defects. We deposited the films on silver interdigitated electrodes on Kapton substrate and submitted them to thermal treatment. Next, we measured the sensitive properties of the resulting sensors towards specific VOCs by impedance spectroscopy. We obtained the eG- and GO-based electronic nose composed of two eG films- and four GO film-based sensors with variable sensitivity to individual VOCs. The smallest relative change in impedance was 5% for the sensor based on eG film annealed at 180 °C toward 10 ppm formaldehyde, whereas the highest relative change was 257% for the sensor based on two-layers deposited GO film annealed at 200 °C toward 80 ppm ethanol. At 10 ppm VOC, the GO film-based sensors were sensitive enough to distinguish between individual VOCs, which implied excellent selectivity, as confirmed by Principle Component Analysis (PCA). According to a PCA-Support Vector Machine-based signal processing method, the electronic nose provided identification accuracy of 100% for individual VOCs. The proposed electronic nose can be used to detect multiple VOCs selectively because each sensor is sensitive to VOCs and has significant cross-selectivity to others.