bluuebunny/crossref_metadata_2025 huggingface dataset:
A Plastic Contamination Image Dataset for Deep Learning Model Development and Training
Authors: Pelletier Mathew G. , Holt Greg A. , Wanjura John D.
Published: May 2020

The removal of plastic contamination in cotton lint is an issue of top priority for the U.S. cotton industry. One of the main sources of plastic contamination appearing in marketable cotton bales is plastic used to wrap cotton modules on cotton harvesters. To help mitigate plastic contamination at the gin, automatic inspection systems are needed to detect and control removal systems. Due to significant cost constraints in the U.S. cotton ginning industry, the use of low-cost color cameras for detection of plastic contamination has been successfully adopted. However, some plastics of similar color to background are difficult to detect when utilizing traditional machine learning algorithms. Hence, current detection/removal system designs are not able to remove all plastics and there is still a need for better detection methods. Recent advances in deep learning convolutional neural networks (CNNs) show promise for enabling the use of low-cost color cameras for detection of objects of interest when placed against a background of similar color. They do this by mimicking the human visual detection system, focusing on differences in texture rather than color as the primary detection paradigm. The key to leveraging the CNNs is the development of extensive image datasets required for training. One of the impediments to this methodology is the need for large image datasets where each image must be annotated with bounding boxes that surround each object of interest. As this requirement is labor-intensive, there is significant value in these image datasets. This report details the included image dataset as well as the system design used to collect the images. For acquisition of the image dataset, a prototype detection system was developed and deployed into a commercial cotton gin where images were collected for the duration of the 2018–2019 ginning season. A discussion of the observational impact that the system had on reduction of plastic contamination at the commercial gin, utilizing traditional color-based machine learning algorithms, is also included.
The Use of a Tomato Landrace as Rootstock Improves the Response of Commercial Tomato under Water Deficit Conditions
Authors: Fullana-Pericàs Mateu , Conesa Miquel À. , Ribas-Carbó Miquel , Galmés Jeroni
Published: May 2020

Grafting onto drought tolerant rootstocks has been proposed as a useful strategy to overcome future water scarcity periods. The ‘de Ramellet’ tomato is a drought tolerant landrace selected under semiarid Mediterranean summer conditions under rain-fed or low irrigation. In this manuscript, the responses of a commercial hybrid ‘de Ramellet’ genotype grafted onto a traditional ‘de Ramellet’ (RL) and a commercial Maxifort (Mx) tomato rootstocks under commercial greenhouse conditions are studied. Non-grafted (NON) and self-grafted (SELF) plants were used as controls. Two water regimes were established: well-watered (WW, covering plant water demands) and water deficit (WD, reducing 50% irrigation as compared to WW). The results confirm an improvement in agronomic performance of Mx as compared to NON, but also show a similar improving effect of RL. Grafting enhanced plant growth regardless of the rootstock under WW conditions. Similarly, water-use efficiency (assessed as leaf carbon isotope composition) increased in grafted plants under WD treatment as compared to NON. Despite the lack of significant differences, RL tended to promote higher fruit production and fruit number than Mx, irrespective of the water treatment, whereas RL was the single graft combination with higher fruit production than NON under WD. In conclusion, the results uncover the potential of drought-adapted landraces to be used as rootstocks in order to increase plant growth and fruit production under both well-watered and water deficit cultivation conditions.
The Implications of EU Regulation 2016/429 on Neglected Diseases of Small Ruminants including Contagious Agalactia with Particular Reference to Italy
Authors: Loria Guido Ruggero , Ruocco Luigi , Ciaccio Gabriele , Iovino Francesco , Nicholas Robin A. J. , Borrello Silvio
Published: May 2020

After almost 40 years, the 27 member states (MS) of the European Union (EU) will comply with the European Law 429/2016 in 2021 by completing a process of unification and harmonization of all regulations related to animal health between MS. These new provisions are based on modern scientific principles on animal health, on long-term epidemiological data, and, above all, on the most current risk assessment and analysis. The paper describes all changes and updates, which will impact the Italian current National regulation. A total of 58 animal diseases have been included in the Annex II (“Listing”) and Annex IV (“Categorization”) of the new Delegated Act (DA 2018/1629). Five diseases comprising the great viral epizooties were automatically included on the list because of their primary importance. These diseases include foot and mouth disease (FMD), African swine fever (ASF), classical swine fever (CSF), highly pathogenic avian influenza (HPAI), and African horse sickness (AHS). Another 53 diseases have been identified by the ad hoc assessment on listing and categorization of animal diseases developed by the European Food Safety Association. Seventeen communicable diseases of the Order Artiodactlya (sheep, goats, deer, etc.) have been listed including foot and mouth disease, sheep and goat pox, and pestes de petits ruminants. In addition, other endemic diseases affecting more than one species include blue tongue, tuberculosis, brucellosis, and anthrax. There are five categories (A-E) based on the degree of action to be undertaken throughout the EU for each disease. These vary from complete eradication for diseases not normally found in the EU like FMD (category A) for establishing surveillance for diseases like West Nile that present high risk but lack control tools (category E).
Mutation Rate Model Used in the DNA VIEW Program
Authors: Krajka Tomasz
Published: May 2020

The first problem considered in this paper is the problem of correctness of a mutation model used in the DNA VIEW program. To this end, we theoretically predict population allele frequency changes in time according to this and similar models (we determine the limit frequencies of alleles—they are uniformly distributed). Furthermore, we evaluate the speed of the above changes using computer simulation applied to our DNA database. Comparing uniformly distributed allele frequencies with these existing in the population (for example, using entropy), we conclude that this mutation model is not correct. The evolution does not follow this direction (direction of uniformly distributed frequencies). The second problem relates to the determination of the extent to which an incorrect mutation model can disturb DNA VIEW program results. We show that in typical computations (simple paternity testing without maternal mutation) this influence is negligible, but in the case of maternal mutation, this should be taken into account. Furthermore, we show that this model is inconsistent from a theoretical viewpoint. Equivalent methods result in different error levels.
Hybrid Fault Diagnosis of Bearings: Adaptive Fuzzy Orthonormal-ARX Robust Feedback Observer
Authors: Piltan Farzin , Kim Jong-Myon
Published: May 2020

Rolling-element bearings (REBs) make up a class of non-linear rotating machines that can be applied in several activities. Conceding a bearing has complicated and uncertain behavior that exhibits substantial challenges to fault diagnosis. Thus, the offered anomaly-diagnosis algorithm, based on a fuzzy orthonormal-ARX adaptive fuzzy logic-structure feedback observer, is developed. A fuzzy orthonormal-ARX algorithm is presented for non-stationary signal modeling. Next, a robust, stable, reliable, and accurate observer is developed for signal estimation. Therefore, firstly, a classical feedback observer is implemented. To address the robustness drawback found in feedback observers, a multi-structure technique is developed. Furthermore, to generate signal estimation performance and reliability, the fuzzy logic technique is applied to the structure feedback observer. Also, to improve the stability, reliability, and robustness of the fuzzy orthonormal-ARX fuzzy logic-structure feedback observer, an adaptive algorithm is developed. After generating the residual signals, a support vector machine (SVM) is developed for the detection and classification of the bearing fault conditions. The effectiveness of the proposed procedure is validated using two different datasets for single-type fault diagnosis based on the Case Western Reverse University (CWRU) vibration dataset and multi-type fault diagnosis of bearing using the Smart Health Safety Environment (SHSE) Lab acoustic emission dataset. The proposed algorithm increases the classification accuracy from 86% in the SVM-based fuzzy orthonormal-ARX feedback observer to 97.5% in single-type fault and from 80% to 98.3% in the multi-type faults.
An ANN-Based Approach for Prediction of Sufficient Seismic Gap between Adjacent Buildings Prone to Earthquake-Induced Pounding
Authors: Khatami Seyed Mohammad , Naderpour Hosein , Razavi Seyed Mohammad Nazem , Barros Rui Carneiro , Sołtysik Barbara , Jankowski Robert
Published: May 2020

Earthquake-induced structural pounding may cause major damages to structures, and therefore it should be prevented. This study is focused on using an artificial neural network (ANN) method to determine the sufficient seismic gap in order to avoid collisions between two adjacent buildings during seismic excitations. Six lumped mass models of structures with a different number of stories (from one to six) have been considered in the study. The earthquake characteristics and the parameters of buildings have been defined as inputs in the ANN analysis. The required seismic gap preventing pounding has been firstly determined for specified structural arrangements and earthquake records. In order to validate the method for other structural parameters, the study has been further extended for buildings with different values of height, mass, and stiffness of each story. Finally, the parametric analysis has been conducted for various earthquakes scaled to different values of the peak ground acceleration (PGA). The results of the verification and validation analyses indicate that the determined seismic gaps are large enough to prevent structural collisions, and they are just appropriate for all different structural arrangements, seismic excitations, and structural parameters. The results of the parametric analysis show that the increase in the PGA of earthquake records leads to a substantial, nearly uniform, increase in the required seismic gap between structures. The above conclusions clearly indicate that the ANN method can be successfully used to determine the minimal distance between two adjacent buildings preventing their collisions during different seismic excitations.
Balancing Waste and Nutrient Flows Between Urban Agglomerations and Rural Ecosystems: Biochar for Improving Crop Growth and Urban Air Quality in The Mediterranean Region
Authors: Zabaniotou Anastasia , Stamou Katerina
Published: May 2020

Mediterranean ecosystems are threatened by water and nutrient scarcity and continuous loss of soil organic carbon. Urban agglomerations and rural ecosystems in the Mediterranean region and globally are interlinked through the flows of resources/nutrients and wastes. Contributing to balancing these cycles, the present study advocates standardized biochar as a soil amendment, produced from Mediterranean suitable biowaste, for closing the nutrient loop in agriculture, with parallel greenhouse gas reduction, enhancing air quality in urban agglomerations, mitigating climate change. The study’s scope is the contextualization of pyrolytic conditions and biowaste type effects on the yield and properties of biochar and to shed light on biochar’s role in soil fertility and climate change mitigation. Mediterranean-type suitable feedstocks (biowaste) to produce biochar, in accordance with biomass feedstocks approved for use in producing biochar by the European Biochar Certificate, are screened. Data form large-scale and long-period field experiments are considered. The findings advocate the following: (a) pyrolytic biochar application in soils contributes to the retention of important nutrients for agricultural production, thereby reducing the use of fertilizers; (b) pyrolysis does not release carbon dioxide to the atmosphere, contributing positively to the balance of carbon dioxide emissions to the atmosphere, with carbon uptake by plant photosynthesis; (c) biochar stores carbon in soils, counterbalancing the effect of climate change by sequestering carbon; (d) there is an imperative need to identify the suitable feedstock for the production of sustainable and safe biochar from a range of biowaste, according to the European Biochar Certificate, for safe crop production.
Developing a Robust Defensive System against Adversarial Examples Using Generative Adversarial Networks
Authors: Taheri Shayan , Khormali Aminollah , Salem Milad , Yuan Jiann-Shiun
Published: May 2020

In this work, we propose a novel defense system against adversarial examples leveraging the unique power of Generative Adversarial Networks (GANs) to generate new adversarial examples for model retraining. To do so, we develop an automated pipeline using combination of pre-trained convolutional neural network and an external GAN, that is, Pix2Pix conditional GAN, to determine the transformations between adversarial examples and clean data, and to automatically synthesize new adversarial examples. These adversarial examples are employed to strengthen the model, attack, and defense in an iterative pipeline. Our simulation results demonstrate the success of the proposed method.
A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons
Authors: Harberts Jann , Kusch Max , O’Sullivan John , Zierold Robert , Blick Robert H.
Published: May 2020

Though patch clamping at room temperature is a widely disseminated standard procedure in the electrophysiological community, it does not represent the biological system in mammals at around 37 °C. In order to better mimic the natural environment in electrophysiological studies, we present a custom-built, temperature-controlled patch clamp platform for upright microscopes, which can easily be adapted to any upright patch clamp setup independently, whether commercially available or home built. Our setup can both cool and heat the platform having only small temperature variations of less than 0.5 °C. We demonstrate our setup with patch clamp measurements at 36 °C on Jurkat T lymphocytes and human induced pluripotent stem cell-derived neurons. Passive membrane parameters and characteristic electrophysiological properties, such as the gating properties of voltage-gated ion channels and the firing of action potentials, are compared to measurements at room temperature. We observe that many processes that are not explicitly considered as temperature dependent show changes with temperature. Thus, we believe in the need of a temperature control in patch clamp measurements if improved physiological conditions are required. Furthermore, we advise researchers to only compare electrophysiological results directly that have been measured at similar temperatures since small variations in cellular properties might be caused by temperature alterations.
In Silico Identification of Type III PKS Chalcone and Stilbene Synthase Homologs in Marine Photosynthetic Organisms
Authors: De Luca Daniele , Lauritano Chiara
Published: May 2020

Marine microalgae are photosynthetic microorganisms at the base of the marine food webs. They are characterized by huge taxonomic and metabolic diversity and several species have been shown to have bioactivities useful for the treatment of human pathologies. However, the compounds and the metabolic pathways responsible for bioactive compound synthesis are often still unknown. In this study, we aimed at analysing the microalgal transcriptomes available in the Marine Microbial Eukaryotic Transcriptome Sequencing Project (MMETSP) database for an in silico search of polyketide synthase type III homologs and, in particular, chalcone synthase (CHS) and stilbene synthase (STS), which are often referred to as the CHS/STS family. These enzymes were selected because they are known to produce compounds with biological properties useful for human health, such as cancer chemopreventive, anti-inflammatory, antioxidant, anti-angiogenic, anti-viral and anti-diabetic. In addition, we also searched for 4-Coumarate: CoA ligase, an upstream enzyme in the synthesis of chalcones and stilbenes. This study reports for the first time the occurrence of these enzymes in specific microalgal taxa, confirming the importance for microalgae of these pathways and giving new insights into microalgal physiology and possible biotechnological applications for the production of bioactive compounds.
Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell Models
Authors: Tzoneva Rumiana , Stoyanova Tihomira , Petrich Annett , Popova Desislava , Uzunova Veselina , Momchilova Albena , Chiantia Salvatore
Published: May 2020

Alkylphospholipids are a novel class of antineoplastic drugs showing remarkable therapeutic potential. Among them, erufosine (EPC3) is a promising drug for the treatment of several types of tumors. While EPC3 is supposed to exert its function by interacting with lipid membranes, the exact molecular mechanisms involved are not known yet. In this work, we applied a combination of several fluorescence microscopy and analytical chemistry approaches (i.e., scanning fluorescence correlation spectroscopy, line-scan fluorescence correlation spectroscopy, generalized polarization imaging, as well as thin layer and gas chromatography) to quantify the effect of EPC3 in biophysical models of the plasma membrane, as well as in cancer cell lines. Our results indicate that EPC3 affects lipid–lipid interactions in cellular membranes by decreasing lipid packing and increasing membrane disorder and fluidity. As a consequence of these alterations in the lateral organization of lipid bilayers, the diffusive dynamics of membrane proteins are also significantly increased. Taken together, these findings suggest that the mechanism of action of EPC3 could be linked to its effects on fundamental biophysical properties of lipid membranes, as well as on lipid metabolism in cancer cells.
When Less Is More: Specific Capture and Analysis of Tumor Exosomes in Plasma Increases the Sensitivity of Liquid Biopsy for Comprehensive Detection of Multiple Androgen Receptor Phenotypes in Advanced Prostate Cancer Patients
Authors: Foroni Chiara , Zarovni Natasa , Bianciardi Laura , Bernardi Simona , Triggiani Luca , Zocco Davide , Venturella Marta , Chiesi Antonio , Valcamonico Francesca , Berruti Alfredo
Published: May 2020

We evaluated the advantages and the reliability of novel protocols for the enrichment of tumor extracellular vesicles (EVs), enabling a blood-based test for the noninvasive parallel profiling of multiple androgen receptor (AR) gene alterations. Three clinically relevant AR variants related to response/resistance to standard-of-care treatments (AR-V7 transcript, AR T878A point mutation and AR gene amplification) were evaluated by digital PCR in 15 samples from patients affected by Castration-Resistant Prostate Cancer (CRPC). Plasma was processed to obtain circulating RNA and DNA using protocols based on tumor EVs enrichment through immuno-affinity and peptide-affinity compared to generic extraction kits. Our results showed that immuno-affinity enrichment prior to RNA extraction clearly outperforms the generic isolation method in the detection of AR-V7, also allowing for a distinction between responder (R) and non-responder (NR) patients. The T878A mutation was detected, overall, in nine out of 15 samples and no approach alone was able to reveal mutations in all harboring samples, showing that the employed methods complement each other. AR amplification was detected in the majority of CRPC samples analysed using either cell-free DNA (cfDNA) or exosome isolation kits (80%). We demonstrated that selective isolation of a subset of circulating exosomes enriched for tumor origin, rather than analysis of total plasma exosomes, or total plasma nucleic acids, increases sensitivity and specificity for the detection of specific alterations.
Parental Education and Youth Inhibitory Control in the Adolescent Brain Cognitive Development (ABCD) Study: Blacks’ Diminished Returns
Authors: Assari Shervin
Published: May 2020

Background: Non-Hispanic Black (NHB) youth are at a higher risk of high-risk behaviors compared to non-Hispanic White (NHW) youth. Some of this racial gap is shown to be due to weaker effects of parental educational attainment on reducing the prevalence of behavioral risk factors such as impulsivity, substance use, aggression, obesity, and poor school performance for NHBs, a pattern called Minorities’ Diminished Returns. These diminishing returns may be due to lower than expected effects of parental education on inhibitory control. Aim: We compared NHW and NHB youth for the effect of parental educational attainment on youth inhibitory control, a psychological and cognitive construct that closely predicts high-risk behaviors such as the use of drugs, alcohol, and tobacco. Methods: This was a cross-sectional analysis that included 4188 youth from the Adolescent Brain Cognitive Development (ABCD) study. The independent variable was parental educational attainment. The main outcome was youth inhibitory control measured by the stop-signal task (SST), which was validated by parent reports on the Child Behavior Checklist (CBCL). Results: In race/ethnicity-stratified models, high parental educational attainment was associated with a higher level of inhibitory control for NHB than NHW youth. In the pooled sample, race/ethnicity showed a statistically significant interaction with parental educational attainment on youth inhibitory control suggesting that high parental educational attainment has a smaller boosting effect on inhibitory control for NHB than NHW youth. Conclusion: Parental educational attainment boosts inhibitory control for NHW but not NHB youth. To minimize the racial gap in youth brain development, we need to address societal barriers that diminish the returns of family economic and human resources, particularly parental educational attainment, for racial and ethnic minority youth. Social and public policies should address structural and societal barriers such as social stratification, segregation, racism, and discrimination that hinder NHB parents’ abilities to effectively mobilize their human resources and secure tangible outcomes for their developing youth.
Application of In Vivo Imaging Techniques for Monitoring Natural Killer Cell Migration and Tumor Infiltration
Authors: Gangadaran Prakash , Rajendran Ramya Lakshmi , Ahn Byeong-Cheol
Published: May 2020

In recent years, the use of natural killer (NK) cell-based immunotherapy has shown promise against various cancer types. To some extent therapeutic potential of NK cell-based immunotherapy depends on migration of NK cells towards tumors in animal models or human subjects and subsequent infiltration. Constant improvement in the pharmacological and therapeutic properties of NK cells is driving the performance and use of NK cell-based immunotherapies. In this review, we summarize the molecular imaging techniques used in monitoring the migration and infiltration of NK cells in vivo at preclinical and clinical levels. A review of pros and cons of each molecular imaging modality is done. Finally, we provide our perception of the usefulness of molecular imaging approaches for in vivo monitoring of NK cells in preclinical and clinical scenarios.
Erratum: Boland, P.M., et al. Immunotherapy for Colorectal Cancer, Cancers 2017, 9, 50
Authors: Boland Patrick M. , Ma Wen Wee
Published: May 2020

The authors wish to make the following corrections to this paper [...]
Hypoxia PET Imaging with [18F]-HX4—A Promising Next-Generation Tracer
Authors: Sanduleanu Sebastian , Wiel Alexander M.A. van der , Lieverse Relinde I.Y. , Marcus Damiënne , Ibrahim Abdalla , Primakov Sergey , Wu Guangyao , Theys Jan , Yaromina Ala , Dubois Ludwig J. , Lambin Philippe
Published: May 2020

Hypoxia—a common feature of the majority of solid tumors—is a negative prognostic factor, as it is associated with invasion, metastasis and therapy resistance. To date, a variety of methods are available for the assessment of tumor hypoxia, including the use of positron emission tomography (PET). A plethora of hypoxia PET tracers, each with its own strengths and limitations, has been developed and successfully validated, thereby providing useful prognostic or predictive information. The current review focusses on [18F]-HX4, a promising next-generation hypoxia PET tracer. After a brief history of its development, we discuss and compare its characteristics with other hypoxia PET tracers and provide an update on its progression into the clinic. Lastly, we address the potential applications of assessing tumor hypoxia using [18F]-HX4, with a focus on improving patient-tailored therapies.
Tumor Cell Associated Hyaluronan-CD44 Signaling Promotes Pro-Tumor Inflammation in Breast Cancer
Authors: Witschen Patrice M. , Chaffee Thomas S. , Brady Nicholas J. , Huggins Danielle N. , Knutson Todd P. , LaRue Rebecca S. , Munro Sarah A. , Tiegs Lyubov , McCarthy James B. , Nelson Andrew C. , Schwertfeger Kathryn L.
Published: May 2020

Cancer has been conceptualized as a chronic wound with a predominance of tumor promoting inflammation. Given the accumulating evidence that the microenvironment supports tumor growth, we investigated hyaluronan (HA)-CD44 interactions within breast cancer cells, to determine whether this axis directly impacts the formation of an inflammatory microenvironment. Our results demonstrate that breast cancer cells synthesize and fragment HA and express CD44 on the cell surface. Using RNA sequencing approaches, we found that loss of CD44 in breast cancer cells altered the expression of cytokine-related genes. Specifically, we found that production of the chemokine CCL2 by breast cancer cells was significantly decreased after depletion of either CD44 or HA. In vivo, we found that CD44 deletion in breast cancer cells resulted in a delay in tumor formation and localized progression. This finding was accompanied by a decrease in infiltrating CD206+ macrophages, which are typically associated with tumor promoting functions. Importantly, our laboratory results were supported by human breast cancer patient data, where increased HAS2 expression was significantly associated with a tumor promoting inflammatory gene signature. Because high levels of HA deposition within many tumor types yields a poorer prognosis, our results emphasize that HA-CD44 interactions potentially have broad implications across multiple cancers.
Ibrutinib Resistance Mechanisms and Treatment Strategies for B-Cell Lymphomas
Authors: George Bhawana , Mullick Chowdhury Sayan , Hart Amber , Sircar Anuvrat , Singh Satish Kumar , Nath Uttam Kumar , Mamgain Mukesh , Singhal Naveen Kumar , Sehgal Lalit , Jain Neeraj
Published: May 2020

Chronic activation of B-cell receptor (BCR) signaling via Bruton tyrosine kinase (BTK) is largely considered to be one of the primary mechanisms driving disease progression in B–Cell lymphomas. Although the BTK-targeting agent ibrutinib has shown promising clinical responses, the presence of primary or acquired resistance is common and often leads to dismal clinical outcomes. Resistance to ibrutinib therapy can be mediated through genetic mutations, up-regulation of alternative survival pathways, or other unknown factors that are not targeted by ibrutinib therapy. Understanding the key determinants, including tumor heterogeneity and rewiring of the molecular networks during disease progression and therapy, will assist exploration of alternative therapeutic strategies. Towards the goal of overcoming ibrutinib resistance, multiple alternative therapeutic agents, including second- and third-generation BTK inhibitors and immunomodulatory drugs, have been discovered and tested in both pre-clinical and clinical settings. Although these agents have shown high response rates alone or in combination with ibrutinib in ibrutinib-treated relapsed/refractory(R/R) lymphoma patients, overall clinical outcomes have not been satisfactory due to drug-associated toxicities and incomplete remission. In this review, we discuss the mechanisms of ibrutinib resistance development in B-cell lymphoma including complexities associated with genomic alterations, non-genetic acquired resistance, cancer stem cells, and the tumor microenvironment. Furthermore, we focus our discussion on more comprehensive views of recent developments in therapeutic strategies to overcome ibrutinib resistance, including novel BTK inhibitors, clinical therapeutic agents, proteolysis-targeting chimeras and immunotherapy regimens.
Room-Temperature Nitrophenol Reduction over Ag–CeO2 Catalysts: The Role of Catalyst Preparation Method
Authors: Chernykh Mariia , Mikheeva Natalia , Zaikovskii Vladimir , Salaev Mikhail , Liotta Leonarda F. , Mamontov Grigory
Published: May 2020

Ag–CeO2 catalysts (20 mol % Ag) were synthesized using different techniques (co-precipitation, impregnation, and impregnation of pre-reduced ceria), characterized by XRD, N2 sorption, TEM, H2-TPR methods, and probed in room-temperature p-nitrophenol reduction into p-aminophenol in aqueous solution at atmospheric pressure. The catalyst preparation method was found to determine the textural characteristics, the oxidation state and distribution of silver and, hence, the catalytic activity in the p-nitrophenol reduction. The impregnation technique was the most favorable for the formation over the ceria surface of highly dispersed silver species that are active in the p-nitrophenol reduction (the first-order rate constant k = 0.656 min−1).
Computational Tools and Resources Supporting CRISPR-Cas Experiments
Authors: Sledzinski Pawel , Nowaczyk Mateusz , Olejniczak Marta
Published: May 2020

The CRISPR-Cas system has become a cutting-edge technology that revolutionized genome engineering. The use of Cas9 nuclease is currently the method of choice in most tasks requiring a specific DNA modification. The rapid development in the field of CRISPR-Cas is reflected by the constantly expanding ecosystem of computational tools aimed at facilitating experimental design and result analysis. The first group of CRISPR-Cas-related tools that we review is dedicated to aid in guide RNA design by prediction of their efficiency and specificity. The second, relatively new group of tools exploits the observed biases in repair outcomes to predict the results of CRISPR-Cas edits. The third class of tools is developed to assist in the evaluation of the editing outcomes by analysis of the sequencing data. These utilities are accompanied by relevant repositories and databases. Here we present a comprehensive and updated overview of the currently available CRISPR-Cas-related tools, from the perspective of a user who needs a convenient and reliable means to facilitate genome editing experiments at every step, from the guide RNA design to analysis of editing outcomes. Moreover, we discuss the current limitations and challenges that the field must overcome for further improvement in the CRISPR-Cas endeavor.
The Functional Role of CONNEXIN 26 Mutation in Nonsyndromic Hearing Loss, Demonstrated by Zebrafish Connexin 30.3 Homologue Model
Authors: Su Hsuan-An , Lai Ting-Wei , Li Shuan-Yow , Su Tzu-Rong , Yang Jiann-Jou , Su Ching-Chyuan
Published: May 2020

Nonsyndromic hearing loss (NSHL) is of great clinical importance, and mutations in the GJB2 gene and the encoded human CONNEXIN 26 (CX26) protein play important roles in the genetic pathogenesis. The CX26 p.R184Q mutation was shown to be a dominant-negative effect in our previous study. Previously, we also demonstrated that zebrafish Cx30.3 is orthologous to human CX26. In the present study, we established transgenic zebrafish models with mutated Cx30.3 specifically expressed in the supporting cells of zebrafish inner ears driven by the agr2 promoter, to demonstrate and understand the mechanism by which the human CX26 R.184 mutation causes NSHL. Our results indicated that significant structural changes in the inner ears of transgenic lines with mutations were measured and compared to wild-type zebrafish. Simultaneously, significant alterations of transgenic lines with mutations in swimming behavior were analyzed with the zebrafish behavioral assay. This is the first study to investigate the functional results of the CX26 p.R184Q mutation with in vivo disease models. Our work supports and confirms the pathogenic role of the CX26 p.R184Q mutation in NSHL, with a hypothesized mechanism of altered interaction among amino acids in the connexins.
Blood and Cancer: Cancer Stem Cells as Origin of Hematopoietic Cells in Solid Tumor Microenvironments
Authors: Hassan Ghmkin , Seno Masaharu
Published: May 2020

The concepts of hematopoiesis and the generation of blood and immune cells from hematopoietic stem cells are some steady concepts in the field of hematology. However, the knowledge of hematopoietic cells arising from solid tumor cancer stem cells is novel. In the solid tumor microenvironment, hematopoietic cells play pivotal roles in tumor growth and progression. Recent studies have reported that solid tumor cancer cells or cancer stem cells could differentiate into hematopoietic cells. Here, we discuss efforts and research that focused on the presence of hematopoietic cells in tumor microenvironments. We also discuss hematopoiesis from solid tumor cancer stem cells and clarify the notion of differentiation of solid tumor cancer stem cells into non-cancer hematopoietic stem cells.
On the Host Side of the Hepatitis E Virus Life Cycle
Authors: Oechslin Noémie , Moradpour Darius , Gouttenoire Jérôme
Published: May 2020

Hepatitis E virus (HEV) infection is one of the most common causes of acute hepatitis in the world. HEV is an enterically transmitted positive-strand RNA virus found as a non-enveloped particle in bile as well as stool and as a quasi-enveloped particle in blood. Current understanding of the molecular mechanisms and host factors involved in productive HEV infection is incomplete, but recently developed model systems have facilitated rapid progress in this area. Here, we provide an overview of the HEV life cycle with a focus on the host factors required for viral entry, RNA replication, assembly and release. Further developments of HEV model systems and novel technologies should yield a broader picture in the future.
Facile Fabrication of Methylcellulose/PLA Membrane with Improved Properties
Authors: Guo Panjie , Wang Fen , Duo Tongtong , Xiao Zhihong , Xu Airong , Liu Rukuan , Jiang Chaohui
Published: May 2020

With the rapid exhaustion of fossil resources, and environmental pollution relative to the use of fossil-based products, developing eco-friendly products using biomass and/or biodegradable resources is becoming increasingly conspicuous. In this study, ecofriendly and biodegradable composite membranes containing varying MC/PLA (methylcellulose/polylactic acid) mass ratios were prepared. The properties and structures of the MC/PLA membranes were studied by mechanical testing, 13C NMR techniques, X-ray diffraction (XRD), thermogravimetric analysis (TGA), and hot compression. The MC/PLA membranes displayed markedly improved tensile strength and elongation at the MC/PLA mass ratio range of 99:1 to 9:1. The tensile strength and elongation of the MC/PLA (97:3) membrane was found to be the optimum, at 30% and 35% higher than the neat MC, respectively. It was also found that hot compression could improve the tensile strength and elongation of the membranes. At the same time, the membranes showed enough good thermal stability. In addition, the effect of MC/PLA mass ratio on morphologies of the membranes were studied by microscopy technique.
Micelle Encapsulation of Ferromagnetic Nanoparticles of Iron Carbide@Iron Oxide in Chitosan as Possible Nanomedicine Agent
Authors: Sauceda-Oloño Perla Yazmin , Cardenas-Sanchez Hector , Argüelles-Pesqueira Anya Isabel , Gutierrez-Valenzuela Cindy , Alvarez-Ramos Mario Enrique , Lucero-Acuña Armando , Zavala-Rivera Paul
Published: May 2020

In this work, the synthesis and characterization of core/shell nanoparticles of iron carbide@iron oxide (Fe3C/γ-Fe2O3) encapsulated into micelles of sodium dodecylsulfate and oleic acid and stabilized with chitosan was developed. The materials were sonosynthesized at low intensities using standard ultrasonic baths with iron pentacarbonyl (Fe(CO)5) and oleic acid as iron source and hydrophobic stabilizer, respectively; obtaining nanoparticles with a hydrodynamic diameter of 19.71 nm and polydispersive index (PDI) of 0.13. The iron carbide@iron oxide nanoparticles (ICIONPs) in oleic acid were used as the organic phase during the self-assemble of nanoemulsion with sodium dodecylsulfate in water to obtain the metastable micelles. The final step involved the stabilization of the micelles using low molecular weight chitosan solution at 2% in acetic acid by ultrasonication bath. The nanosystem showed a hydrodynamic diameter of 185.30 nm, a PDI of 0.15 with a superficial charge ζ of 36.70 mV. Due to the magnetic, physical and chemical properties previously measured of the ICIONPs, it is believed that this type of nanoparticles can be used as a possible nanomedicine agent.
Narrow/Broad-Band Absorption Based on Water-Hybrid Metamaterial
Authors: Khuyen Bui Xuan , Hanh Vu Thi Hong , Tung Bui Son , Lam Vu Dinh , Kim Young Ju , Lee YoungPak , Tu Hua-Tian , Chen Liang Yao
Published: May 2020

In this work, the possibility of a switchable metamaterial absorber is proposed to control absorption bandwidth in the WiMAX/LTE (worldwide interoperability for microwave access/long term evolution) band, by taking advantage of the low cost and myriad structural configurations afforded by water-based metamaterials. By exploiting truncated cone-type resonators, the fractional bandwidth of 27.6% of absorption spectrum can be adjusted flexibly to be 7.4% of the narrow-band absorption depending on the volume of injected water, in both simulation and experiment at room temperature. In particular, this control method can be applied stably for different temperature of injected water. We describe a dynamic mechanism for broadband MA, as well as a principle for controlling the absorption characteristics utilizing a combination of magnetic resonance and perfect impedance matching. These results are a stepping-stone towards the realization of smart electronics integrated with multi-functional metamaterials in military, biomedical, communication and other fields.
Modeling of Laboratory Steam Methane Reforming and CO2 Methanation Reactors
Authors: Costamagna Paola , Pugliese Federico , Cavattoni Tullio , Busca Guido , Garbarino Gabriella
Published: May 2020

To support the interpretation of the experimental results obtained from two laboratory-scale reactors, one working in the steam methane reforming (SMR) mode, and the other in the CO2 hydrogenation (MCO2) mode, a steady-state pseudo-homogeneous 1D non-isothermal packed-bed reactor model is developed, embedding the classical Xu and Froment local kinetics. The laboratory reactors are operated with three different catalysts, two commercial and one homemade. The simulation model makes it possible to identify and account for thermal effects occurring inside the catalytic zone of the reactor and along the exit line. The model is intended to guide the development of small size SMR and MCO2 reactors in the context of Power-to-X (P2X) studies.
Improved Blade Design for Tidal Current Turbines
Authors: Liu Hongwei , Gu Yajing , Lin Yong-Gang , Li Yang-Jian , Li Wei , Zhou Hongbin
Published: May 2020

The efficiency and reliability of blades are key indicators for a tidal current turbine (TCT). A traditional blade design is often an empirical design on hydrodynamics and structure, sequentially. A hydrofoil with a low lift–drag ratio is considered when the structural strength cannot satisfy requirements. However, efficiency is then sacrificed. A redundant design is generally adopted to protect from load uncertainty, but this increases blade weight and cost. In this paper, we present an improved blade design method to enhance the blade reliability of TCTs with relatively high efficiency for energy generation. An equivalent S–N curve model describing the relationship between axial load and cycle times and a simplified load spectrum including load caused by shear flow and turbulence are proposed for the first time for convenient lifetime estimation. A multi-objective genetic algorithm is used to optimize the chord length, twist angle, and thickness of the blade for the best match between lifetime and efficiency. This blade design method was conducted on a 4.4 m length blade of a 120 kW TCT. Comparisons between the original design and optimal designs indicate that the comprehensive performance in terms of the hydrodynamics, structure, and lifetime of the blade presented significant improvements with a small, acceptable efficiency loss. The results also provide more alternative blade solutions for developers as a reference.
Analysis of Internal Overvoltages in Transformer Windings during Transients in Electrical Networks
Authors: Furgał Jakub , Kuniewski Maciej , Pająk Piotr
Published: May 2020

Due to the increasing requirements for the reliability of electrical power supply and associated apparatus, it is necessary to provide a detailed analysis of the overvoltage risk of power transformer insulation systems and equipment connected to their terminals. Exposure of transformer windings to overvoltages is the result of the propagation condition of electromagnetic waves in electrical networks and transformer windings. An analysis of transformer winding responses to transients in power systems is of particular importance, especially when protection against surges by typical overvoltage protection systems is applied. The analysis of internal overvoltages in transformers during a typical transient related to switching operations and selected failures is of great importance, particularly to assess the overvoltage exposure of insulation systems in operating conditions. The random nature of overvoltage phenomena in electrical networks implies the usage of computer simulations for the analysis of overvoltage exposures of electrical devices in operation. This article presents the analysis of the impact of transient phenomena in a model of a medium-voltage electrical network during switching operations and ground faults on overvoltages in the internal insulation systems of transformer windings. The basis of the analysis is simulations of overvoltages in the windings, made in the Electromagnetic Transients Program/Alternative Transients Program (EMTP/ATP) using a model with lumped parameters of transformer windings. The analysis covers the impact of the cable line length and the ground fault resistance value on internal overvoltage distributions.
What Attributes Do Passengers Value in Electrified Buses?
Authors: Kwon Yeongmin , Kim Suji , Kim Hyungjoo , Byun Jihye
Published: May 2020

The Korean government has announced plans to supply electrified buses to achieve decarbonization in the transportation sector and to create next-generation growth engines. Although a multitude of technical and political studies have been conducted to support the successful introduction of electrified buses, studies on the attitudes and perceptions of passengers toward electrified buses remain insufficient. To evaluate the perceptions and preferences of potential passengers toward the specific attributes of electrified buses, this study performed an online survey (N = 586) that includes people who had experienced travel on public buses. Values of the relative importance of eight different attributes—safety, ride comfort, environmental friendliness, exterior design, cleanliness, crowding, seat comfort, and convenience getting on/off—were evaluated using the best-worst scaling method. The results showed that safety (share of preference: 41.3%) was the most important attribute when using electrified buses. This was followed by eco-friendliness (14.3%) and ride comfort (13.6%). On the other hand, the least important attribute was exterior design (1.8%). Gender differences were also observed in the valuation of certain attributes among the passenger preferences toward electrified buses. The results of this study contribute to the development of strategies for the wide-spread adoption of electrified buses and provide a stepping-stone to a more sustainable public transportation system.
Investigation of an Innovative Rotor Modification for a Small-Scale Horizontal Axis Wind Turbine
Authors: Bugała Artur , Roszyk Olga
Published: May 2020

This paper presents the results of the computational fluid dynamics (CFD) simulation of the airflow for a 300 W horizontal axis wind turbine, using additional structural elements which modify the original shape of the rotor in the form of multi-shaped bowls which change the airflow distribution. A three-dimensional CAD model of the tested wind turbine was presented, with three variants subjected to simulation: a basic wind turbine without the element that modifies the airflow distribution, a turbine with a plano-convex bowl, and a turbine with a centrally convex bowl, with the hyperbolic disappearance of convexity as the radius of the rotor increases. The momentary value of wind speed, recorded at measuring points located in the plane of wind turbine blades, demonstrated an increase when compared to the base model by 35% for the wind turbine with the plano-convex bowl, for the wind speed of 5 m/s, and 31.3% and 49% for the higher approaching wind speed, for the plano-convex bowl and centrally convex bowl, respectively. The centrally convex bowl seems to be more appropriate for higher approaching wind speeds. An increase in wind turbine efficiency, described by the power coefficient, for solutions with aerodynamic bowls was observed.