bluuebunny/crossref_metadata_2025 huggingface dataset:
Overview of Taken Initiatives and Adaptation Measures in Polish Mining Companies during a Pandemic
Authors: Kowal Barbara , Ranosz Robert , Herezy Łukasz , Cichy Wojciech , Świniarska Olga , Domaracka Lucia
Published: September 2022

The emergence of the new SARS-CoV-2 virus two years ago strongly affected economic life and labour markets around the world. The pandemic affected many sectors, including the mining industry. Coal companies have had to cope with the challenges and adapt their operations to the situation. Due to the peculiarities of the mines, not only to the hazardous factors and conditions prevailing underground but also to the large number of employees who usually move in groups in the plants, the emergence of a new threat caused by a biological agent posed a real challenge for them. The aim of this paper was to present the initiatives and measures taken in the coal mining sector to ensure the safety of workers during a pandemic. The guidelines for the operation of mining plants during the SARS-CoV-2 epidemic were analysed, as well as the identification of locations in mining plants particularly vulnerable to infection with the virus. We also presented how the pandemic period affected the operations of a selected coal company in Poland from an economic point of view.
Simplified Modeling and Analysis of Surface Temperature Distribution in Electrically Heated Catalyst for Diesel Urea-SCR Systems
Authors: Moon Seoksu , Park Sunhong , Son Jihyun , Oh Kwangchul , Jang Sungwook
Published: September 2022

Impending emission regulations of diesel engines for construction machineries would regulate nitrogen oxide emissions strictly in cold operating conditions. The urea-based selective catalytic reduction (urea-SCR) system coupled with the electrically heated catalyst (EHC) has been considered as a potential measure to meet the strict emission regulations by promoting evaporation and thermal decomposition of urea–water solution in cold operating conditions. Analyzing the thermal conditions in the EHC is crucial for the optimized operation and control of EHC-based urea-SCR systems under various engine operating conditions. In the current study, we introduce a simple one-dimensional analysis scheme to characterize the surface temperature distribution in the EHC based on energy conservation and the theories of forced internal convection. Since the EHC has a complicated internal structure with fine flow cells inside it, a flow cell in the EHC is extracted for the one-dimensional heat transfer analysis. EHC operation parameters such as exhaust gas flow rate and supplied electric power to the EHC are scaled to be applied for the flow cell analysis. The adequacy of the analysis scheme is then validated by surface temperature measurement results at the EHC outlet. The validation results showed over 95% prediction accuracy of the 1D analysis scheme in the operating conditions of a heavy-duty diesel engine. Based on proven reliability, the effects of geometric and operation parameters on the surface temperature distribution in the EHC were analyzed and discussed using the analysis results.
Ineffectiveness of Antiresorptive Agent Drug Holidays in Osteoporosis Patients for Treatment of Medication-Related Osteonecrosis of the Jaw: Consideration from Immunohistological Observation of Osteoclast Suppression and Treatment Outcomes
Authors: Omori Keisuke , Otsuru Mitsunobu , Morishita Kota , Hayashida Saki , Suyama Koki , Naruse Tomofumi , Soutome Sakiko , Umeda Masahiro
Published: September 2022

In patients with osteoporosis receiving antiresorptive agents (ARs), it has been widely practiced to withdraw ARs for several months before tooth extraction and during treatment if medication-related osteonecrosis of the jaw (MRONJ) develops. This study examined the effects of drug holidays on recovery from osteoclast suppression and the treatment outcomes. The relationship between the period of the drug holidays and treatment outcomes was examined retrospectively in 166 osteoporosis patients with MRONJ who received ARs. Histological examinations using hematoxylin and eosin staining and cathepsin K stains were performed to observe the recovery from osteoclast suppression in 43 patients in whom living bone was observed in the resection margins of the surgical specimens. Three-month AR drug holidays were not significantly correlated with the treatment outcomes of the 139 patients who underwent surgical treatment and the 27 who underwent conservative treatment. Of the 43 patients who underwent histological investigations, 16 had drug holidays from 7 to 678 days. Osteoclast suppression was observed in almost all patients, except in one without a drug holiday and one with a 261-day drug holiday. These findings suggest that AR drug holidays for approximately 3 months neither recover osteoclast suppression nor affect treatment outcomes.
Does Eco-Compensation Alleviate Rural Poverty? New Evidence from National Key Ecological Function Areas in China
Authors: Qin Bingtao , Yu Yongwei , Ge Liming , Yang Le , Guo Yuanguo
Published: September 2022

The Transfer Payment Policy of National Key Ecological Functional Areas (TPEFAP), a well-known ecological compensation (eco-compensation) scheme in China, has been proposed by the government to alleviate ecological poverty and protect the environment. In literature, the effectiveness of the TPEFAP on environmental conservation has been widely examined, while few pay attention to the effect of the TPEFAP on poverty alleviation, especially with the consideration of its spatial spillovers as well. In this paper, we utilize panel data covering the key ecological functional areas of China during the period 2011–2018 to evaluate the impact of the TPEFAP on poverty alleviation and also its spatial spillovers by employing the synthetic control method (SCM) and the dynamic spatial Durbin model, respectively. Specifically, we apply the entropy weight method (EWM) to calculate the multidimensional poverty index (MPI) and measure pro-poor effect in terms of MPI change. The results show that: (1) TPEFAP has stable positive effects on MPI in Hubei, Yunnan, Jilin, Gansu, and Ningxia, while the impact on Qinghai fluctuates. (2) MPI presents a significant spatial correlation. Furthermore, both the direct and indirect effects of TPEFAP on MPI are significant and stable positive, for both short- or long-term. (3) For potential channels, rural non-farm employment, rural labor mobility, and agricultural productivity are the key pathways through which the TPEFAP can alleviate poverty both in local and adjacent provinces. However, it is difficult to find significant positive spatial spillovers for the TPEFAP if only the natural resources scale is considered. This study indicates that the government should pay attention to the policy expectations of ecological poverty alleviation and, in future eco-compensation, must further increase the coverage of subsidies and diversify the forms of subsidies.
A Scoping Review on Biopsychosocial Predictors of Mental Health among Older Adults
Authors: Murniati Nia , Al Aufa Badra , Kusuma Dian , Kamso Sudijanto
Published: September 2022

This review aims to map the available evidence on biopsychosocial predictors of elderly mental health. The articles were independently screened in three selected databases, namely Pubmed, Proquest and Google Scholar. The stages consist of identifying the research questions, seeking and selecting relevant evidence, mapping data, and concluding and reporting results. The PRISMA flowchart was used to show the PEOS evidence search flow. A total of 23,722 articles were obtained from all databases during the initial search, where 458 titles fulfilled the eligibility criteria at the title screening stage. Furthermore, 383 articles passed through abstract screening, where 75 met the inclusion criteria and were included for full-text screening. Based on the full-text screening stage, 28 articles were excluded and the remaining 47 articles that matched the search process were included for data extraction. This review creates biopsychosocial variables related to the mental health of the elderly. The biological factors consist of age, biomarkers, female, health conditions, chronic diseases, and physical function. Variables related to psychological factors are affect, personality traits, and subjective well-being. Meanwhile, social factors include smoking, sleep quality, physical activity, daily living, social support, marital status, loneliness, religiosity, spirituality, and early life conditions.
An Exploratory Trial of Brief Mindfulness-Based Zentangle Art Workshops in Family Social Services during COVID-19: Transitioning from Offline to Online
Authors: Sit Shirley Man-Man , Ng Ellen , Ho Hilary Pui-Yee , Wong Peony Cheuk-Yeuk , Wang Man-Ping , Ho Sai-Yin , Lam Tai-Hing , Lai Agnes Yuen-Kwan
Published: September 2022

Mindfulness-based art therapy has shown to improve psychological well-being. Zentangle is an easy-to-learn, mindfulness-based art therapy suitable for everyone. We reported the transition from face-to-face to online Zentangle workshops in family social services during COVID-19. We explored feedback from face-to-face workshops and the acceptability of an online approach utilizing information communication technology (ICT) to achieve greater service reach, satisfaction, and knowledge and related outcomes. Under the Hong Kong Jockey Club SMART Family-Link Project and in collaboration with Caritas Integrated Family Service Centre—Aberdeen, this study was conducted in two phases: a four-session, face-to-face workshop (phase one) and eleven online single-session workshops (phase two) from September 2019 to September 2020. A total of 305 participants joined the workshops. Phase one participants (n = 11) reported high satisfaction (4.7 out of 5), increases in knowledge (4.2/5) and confidence (3.9/5) towards managing stress, increases in knowledge (4.1/5) and confidence (3.9/5) in showing support and care towards family members, and an increase in knowledge towards strengthening family relationships (4.0/5). Phase two participants (n = 294) also reported high satisfaction (4.7/5) and strongly agreed that ICT helped with learning Zentangle more conveniently, that they had increased knowledge and interest in Zentangle (all 4.7/5), and would definitely join the workshop again (4.8/5). The qualitative data supported the quantitative findings. We are the first to report on the utilization of ICT in an exploratory trial of brief, online Zentangle art workshops targeting the general public, with high satisfaction and positive participant experiences with ICT integration, learning Zentangle, and enhanced psychological and family well-being. This study provided preliminary evidence on the use of ICT to successfully transition face-to-face to online workshops and reach a wider audience.
Spatiotemporal Heterogeneity and Driving Factors of Water Resource and Environment Carrying Capacity under High-Quality Economic Development in China
Authors: Zhang Qian , Shen Juqin
Published: September 2022

Rapid economic growth and social development in China have led to serious water pollution problems and water resource shortages, limiting the sustainable development that could support both the socio-economy and water resources carrying capacity (WRECC). However, the spatial heterogeneity and evolutionary characteristics of the coordination between the WRECC and economic development have not been adequately explored in China. In this study, we developed the support and pressure indicators of China’s 30 provinces and then analyzed the spatiotemporal distribution and evolution characteristics of their WRECC by using the geographically and temporally weighted regression (GTWR) model. The main findings are shown in the following: (i) From a temporal perspective, there has been an overall upward trend in the WRECC to support human activities; however, the WRECC level is not high. Approximately 63.7% of provinces remain in an overloaded state, indicating that the support indicator of most provinces is smaller than the pressure indicator imposed by human social activities. (ii) There are significant spatial differences in the WRECC indicators across provinces. Provinces with low-level WRECCs are concentrated in central China but decrease significantly from the country’s borders to its center. Eastern regions have a medium-level of WRECC with the greatest degree of regional difference, while western regions have a high-level of WRECC with the smallest degree of regional difference. The variation of WRECC is attributed to within-group differences in the three geographical regions in China. (iii) The factors that significantly impact the WRECC include population density, gross domestic product (GDP), temperature, urbanization, the added value of tertiary industry within the GDP, and R&D expenditures. GDP and R&D expenditures positively impact the WRECC, while the other four factors have different influences on the WRECC. (iv) The spatial distributions of driving factors show significant aggregation characteristics, with decreasing trends from the eastern to western regions and from the southern to northern regions. These findings present a comprehensive understanding of the current WRECC in China’s provinces which can be used as a reference for realizing environmentally sustainable water development strategies under high-quality economic development.
Exploring the Driving Factors of Remote Sensing Ecological Index Changes from the Perspective of Geospatial Differentiation: A Case Study of the Weihe River Basin, China
Authors: Zhang Kaili , Feng Rongrong , Zhang Zhicheng , Deng Chun , Zhang Hongjuan , Liu Kang
Published: September 2022

Using the Google Earth Engine (GEE) platform, Moderate-resolution image spectroradiometer (MODIS) data of the Weihe River Basin from 2001 to 2021 were acquired, four ecological indicators, namely, greenness, wetness, heat, and dryness, were extracted, and the remote sensing ecological index (RSEI) was constructed through principal component analysis. In addition, the geographic detectors and a multi-scale geographic weighted regression model (MGWR) were used to identify the main driving factors of RSEI changes and capture the differences in spatial changes from different perspectives using multiple indicators. The results show that (1) the quality of the eco-environment in the Weihe River basin improved as a whole from 2001 to 2021, and the RSEI increased from 0.376 to 0.414. In terms of the RSEI grade, the medium RSEI and high RSEI areas increased significantly and the growth rate increased significantly, reaching 26.42% and 27.70%, respectively. (2) Spatially, the quality of the eco-environment in the Weihe River Basin exhibited a spatial distribution pattern that was high in the south and low in the north, among which the quality of the eco-environment in the Weihe River Basin in northern Shaanxi and northwestern Ningxia and Gansu was relatively low. In addition, during the study period, the RSEI of the Qinling Mountains in the southern part of the Weihe River Basin and the Jinghe River and Luohe River areas improved significantly. The urban area on the Guanzhong Plain in the Weihe River Basin experienced rapid economic growth, and urban expansion led to a significant decrease in the quality of the eco-environment. (3) The eco-environment quality in the Weihe River Basin is the result of the interaction of natural, anthropogenic, and landscape pattern factors. All of the interactions between the influencing factors had a stronger influence than those of the individual factors. There were significant differences between the individual drivers and the spatial variation in RSEI, suggesting that different factors dominate the variation in RSEI in different regions, and zonal management is crucial to achieving sustainable management of RSEI. The study shows that to improve the eco-environment quality of the Weihe River Basin, it is necessary to further strengthen ecological protection projects, reasonably allocate landscape elements, and strengthen the resistance and resilience of the ecosystem.
Managing Hospital Employees’ Burnout through Transformational Leadership: The Role of Resilience, Role Clarity, and Intrinsic Motivation
Authors: Chen Jinyong , Ghardallou Wafa , Comite Ubaldo , Ahmad Naveed , Ryu Hyungseo Bobby , Ariza-Montes Antonio , Han Heesup
Published: September 2022

Medical errors have been identified as one of the greatest evils in the field of healthcare, causing millions of patient deaths around the globe each year, especially in developing and poor countries. Globally, the social, economic, and personal impact of medical errors leads to a multi-trillion USD loss. Undoubtedly, medical errors are serious public health concerns in modern times, which could be mitigated by taking corrective measures. Different factors contribute to an increase in medical errors, including employees’ risk of burnout. Indeed, it was observed that hospital employees are more exposed to burnout situations compared to other fields. In this respect, managing hospital employees through transformational leadership (TL) may reduce the risk of burnout. However, surprisingly, studies on the relationship between TL and burnout are scarce in a healthcare system, indicating the existence of a critical knowledge gap. This study aims to fill this knowledge gap by investigating the role of TL in reducing the risk of burnout among hospital employees. At the same time, this study also tests the mediating effects of resilience and role clarity with the conditional indirect effect of intrinsic motivation in the above-proposed relationship. To test different hypotheses, a hypothetical model was developed for which we collected the data from different hospital employees (n = 398). Structural equation modeling (SEM) was considered for statistical validation of hypotheses confirming that TL significantly reduces burnout. The results further indicated that resilience and role clarity mediate this relationship significantly. Lastly, the conditional indirect effect of intrinsic motivation was also confirmed. Our results provide meaningful insights to the hospital administrators to combat burnout, a critical reason for medical errors in hospitals. Further, by incorporating the TL framework, a hospital may reduce the risk of burnout (and, hence, medical errors); on the one hand, such a leadership style also provides cost benefits (reduced medical errors improve cost efficiency). Other different theoretical and practical contributions are discussed in detail.
GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton
Authors: Wang Yanwen , Sun Zhimao , Wang Long , Chen Lingling , Ma Lina , Lv Jiaoyan , Qiao Kaikai , Fan Shuli , Ma Qifeng
Published: September 2022

Block of proliferation 1 (BOP1) is a key protein that helps in the maturation of ribosomes and promotes the progression of the cell cycle. However, its role in the leaf morphogenesis of cotton remains unknown. Herein, we report and study the function of GhBOP1 isolated from Gossypium hirsutum. The sequence alignment revealed that BOP1 protein was highly conserved among different species. The yeast two-hybrid experiments, bimolecular fluorescence complementation, and luciferase complementation techniques revealed that GhBOP1 interact with GhPES and GhWDR12. Subcellular localization experiments revealed that GhBOP1, GhPES and GhWDR12 were localized at the nucleolus. Suppression of GhBOP1 transcripts resulted in the uneven bending of leaf margins and the presence of young wrinkled leaves by virus-induced gene silencing assay. Abnormal palisade arrangements and the presence of large upper epidermal cells were observed in the paraffin sections of the wrinkled leaves. Meanwhile, a jasmonic acid-related gene, GhOPR3, expression was increased. In addition, a negative effect was exerted on the cell cycle and the downregulation of the auxin-related genes was also observed. These results suggest that GhBOP1 plays a critical role in the development of wrinkled cotton leaves, and the process is potentially modulated through phytohormone signaling.
A Mouse Model of Glycogen Storage Disease Type IX-Beta: A Role for Phkb in Glycogenolysis
Authors: Arends Charles J. , Wilson Lane H. , Estrella Ana , Kwon Oh Sung , Weinstein David A. , Lee Young Mok
Published: September 2022

Glycogen storage disease type IX (GSD-IX) constitutes nearly a quarter of all GSDs. This ketotic form of GSD is caused by mutations in phosphorylase kinase (PhK), which is composed of four subunits (α, β, γ, δ). PhK is required for the activation of the liver isoform of glycogen phosphorylase (PYGL), which generates free glucose-1-phosphate monomers to be used as energy via cleavage of the α -(1,4) glycosidic linkages in glycogen chains. Mutations in any of the PhK subunits can negatively affect the regulatory and catalytic activity of PhK during glycogenolysis. To understand the pathogenesis of GSD-IX-beta, we characterized a newly created PHKB knockout (Phkb−/−) mouse model. In this study, we assessed fasting blood glucose and ketone levels, serum metabolite concentrations, glycogen phosphorylase activity, and gene expression of gluconeogenic genes and fibrotic genes. Phkb−/− mice displayed hepatomegaly with lower fasting blood glucose concentrations. Phkb−/− mice showed partial liver glycogen phosphorylase activity and increased sensitivity to pyruvate, indicative of partial glycogenolytic activity and upregulation of gluconeogenesis. Additionally, gene expression analysis demonstrated increased lipid metabolism in Phkb−/− mice. Gene expression analysis and liver histology in the livers of old Phkb−/− mice (>40 weeks) showed minimal profibrogenic features when analyzed with age-matched wild-type (WT) mice. Collectively, the Phkb−/− mouse recapitulates mild clinical features in patients with GSD-IX-beta. Metabolic and molecular analysis confirmed that Phkb−/− mice were capable of sustaining energy homeostasis during prolonged fasting by using partial glycogenolysis, increased gluconeogenesis, and potentially fatty acid oxidation in the liver.
Detailed Analysis of Dorsal-Ventral Gradients of Gene Expression in the Hippocampus of Adult Rats
Authors: Beletskiy Alexander , Positselskaya Ekaterina , Vinarskaya Aliya Kh. , Spivak Yulia S. , Dobryakova Yulia V. , Tyulenev Iliya , Markevich Vladimir A. , Bolshakov Alexey P.
Published: September 2022

We performed RNA sequencing of the dorsal and ventral parts of the hippocampus and compared it with previously published data to determine the differences in the dorsoventral gradients of gene expression that may result from biological or technical variability. Our data suggest that the dorsal and ventral parts of the hippocampus differ in the expression of genes related to signaling pathways mediated by classical neurotransmitters (glutamate, GABA, monoamines, etc.) as well as peptide and Wnt ligands. These hippocampal parts also diverge in the expression of axon-guiding molecules (both receptors and ligands) and splice isoforms of genes associated with intercellular signaling and cell adhesion. Furthermore, analysis of differential expressions of genes specific for astrocytes, microglia, oligodendrocytes, and vascular cells suggests that non-neuronal cells may also differ in the characteristics between hippocampal parts. Analysis of expression of transposable elements showed that depletion of ribosomal RNA strongly increased the representation of transposable elements in the RNA libraries and helped to detect a weak predominance of expression of these elements in the ventral hippocampus. Our data revealed new molecular dimensions of functional differences between the dorsal and ventral hippocampus and points to possible cascades that may be involved in the longitudinal organization of the hippocampus.
Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study
Authors: Al-Zeer Munir A. , Prehn Franziska , Fiedler Stefan , Lienert Ulrich , Krisch Michael , Berg Johanna , Kurreck Jens , Hildebrandt Guido , Schültke Elisabeth
Published: September 2022

Radiotherapy is an important component in the treatment of lung cancer, one of the most common cancers worldwide, frequently resulting in death within only a few years of diagnosis. In order to evaluate new therapeutic approaches and compare their efficiency with regard to tumour control at a pre-clinical stage, it is important to develop standardized samples which can serve as inter-institutional outcome controls, independent of differences in local technical parameters or specific techniques. Recent developments in 3D bioprinting techniques could provide a sophisticated solution to this challenge. We have conducted a pilot project to evaluate the suitability of standardized samples generated from 3D printed human lung cancer cells in radiotherapy studies. The samples were irradiated at high dose rates using both broad beam and microbeam techniques. We found the 3D printed constructs to be sufficiently mechanically stable for use in microbeam studies with peak doses up to 400 Gy to test for cytotoxicity, DNA damage, and cancer cell death in vitro. The results of this study show how 3D structures generated from human lung cancer cells in an additive printing process can be used to study the effects of radiotherapy in a standardized manner.
Hemp-Derived Nanovesicles Protect Leaky Gut and Liver Injury in Dextran Sodium Sulfate-Induced Colitis
Authors: Eom Jung-Young , Choi Sang-Hun , Kim Hyun-Jin , Kim Dong-ha , Bae Ju-Hyun , Kwon Gi-Seok , Lee Dong-hee , Hwang Jin-Hyeon , Kim Do-Kyun , Baek Moon-Chang , Cho Young-Eun
Published: September 2022

Hemp (Cannabis sativa L.) is used for medicinal purposes owing to its anti-inflammatory and antioxidant activities. We evaluated the protective effect of nanovesicles isolated from hemp plant parts (root, seed, hemp sprout, and leaf) in dextran sulfate sodium (DSS)-induced colitis in mice. The particle sizes of root-derived nanovesicles (RNVs), seed-derived nanovesicles (SNVs), hemp sprout-derived nanovesicles (HSNVs), and leaf-derived nanovesicles (LNVs) were within the range of 100–200 nm as measured by nanoparticle tracking analysis. Acute colitis was induced in C57BL/N mice by 5% DSS in water provided for 7 days. RNVs were administered orally once a day, leading to the recovery of both the small intestine and colon lengths. RNVs, SNVs, and HSNVs restored the tight (ZO-1, claudin-4, occludin) and adherent junctions (E-cadherin and α-tubulin) in DSS-induced small intestine and colon injury. Additionally, RNVs markedly reduced NF-κB activation and oxidative stress proteins in DSS-induced small intestine and colon injury. Tight junction protein expression and epithelial cell permeability were elevated in RNV-, SNV-, and HSNV-treated T84 colon cells exposed to 2% DSS. Interestedly, RNVs, SNVs, HSNVs, and LNVs reduced ALT activity and liver regeneration marker proteins in DSS-induced liver injury. These results showed for the first time that hemp-derived nanovesicles (HNVs) exhibited a protective effect on DSS-induced gut leaky and liver injury through the gut–liver axis by inhibiting oxidative stress marker proteins.
LAG-3 as a Potent Target for Novel Anticancer Therapies of a Wide Range of Tumors
Authors: Sauer Natalia , Szlasa Wojciech , Jonderko Laura , Oślizło Małgorzata , Kunachowicz Dominika , Kulbacka Julita , Karłowicz-Bodalska Katarzyna
Published: September 2022

LAG-3 (Lymphocyte activation gene 3) protein is a checkpoint receptor that interacts with LSEC-tin, Galectin-3 and FGL1. This interaction leads to reduced production of IL-2 and IFN-γ. LAG-3 is widely expressed in different tumor types and modulates the tumor microenvironment through immunosuppressive effects. Differential expression in various tumor types influences patient prognosis, which is often associated with coexpression with immune checkpoint inhibitors, such as TIM-3, PD-1 and CTLA-4. Here, we discuss expression profiles in different tumor types. To date, many clinical trials have been conducted using LAG-3 inhibitors, which can be divided into anti-LAG-3 monoclonal antibodies, anti-LAG-3 bispecifics and soluble LAG-3-Ig fusion proteins. LAG-3 inhibitors supress T-cell proliferation and activation by disallowing for the interaction between LAG-3 to MHC-II. The process enhances anti-tumor immune response. In this paper, we will review the current state of knowledge on the structure, function and expression of LAG-3 in various types of cancer, as well as its correlation with overall prognosis, involvement in cell-based therapies and experimental medicine. We will consider the role of compounds targeting LAG-3 in clinical trials both as monotherapy and in combination, which will provide data relating to the efficacy and safety of proposed drug candidates.
Physical, Chemical, and Biological Properties of Chitosan-Coated Alginate Microparticles Loaded with Porcine Interleukin-1β: A Potential Protein Adjuvant Delivery System
Authors: Ho Wan-Xuan , Chen Wen-Ting , Lien Chih-Hsuan , Yang Hsin-Yu , Chen Kuan-Hung , Wei Yu-Fan , Wang Meng-Han , Ko I-Ting , Tseng Fan-Gang , Yin Hsien-Sheng
Published: September 2022

We previously developed chicken interleukin-1β (IL-1β) mutants as single-dose adjuvants that induce protective immunity when co-administered with an avian vaccine. However, livestock such as pigs may require a vaccine adjuvant delivery system that provides long-lasting protection to reduce the need for successive booster doses. Therefore, we developed chitosan-coated alginate microparticles as a carrier for bovine serum albumin (BSA) or porcine IL-1β (pIL-1β) and assessed their physical, chemical, and biological properties. Electrospraying of the BSA-loaded alginate microparticles (BSA/ALG MPs) resulted in an encapsulation efficiency of 50%, and those MPs were then coated with chitosan (BSA/ALG/CHI MPs). Optical and scanning electron microscopy, zeta potential analysis, and Fourier transform infrared spectroscopy were used to characterize these MPs. The BSA encapsulation parameters were applied to ALG/CHI MPs loaded with pIL-1β, which were not cytotoxic to porcine fibroblasts but had enhanced bio-activity over unencapsulated pIL-1β. The chitosan layer of the BSA/ALG/CHI MPs prevented burst release and facilitated sustained release of pIL-1β for at least 28 days. In conclusion, BSA/ALG/CHI MPs prepared as a carrier for pIL-1β may be used as an adjuvant for the formulation of pig vaccines.
Unusual Cytochrome c552 from Thioalkalivibrio paradoxus: Solution NMR Structure and Interaction with Thiocyanate Dehydrogenase
Authors: Britikov Vladimir V. , Bocharov Eduard V. , Britikova Elena V. , Dergousova Natalia I. , Kulikova Olga G. , Solovieva Anastasia Y. , Shipkov Nikolai S. , Varfolomeeva Larisa A. , Tikhonova Tamara V. , Timofeev Vladimir I. , Shtykova Eleonora V. , Altukhov Dmitry A. , Usanov Sergey A. , Arseniev Alexander S. , Rakitina Tatiana V. , Popov Vladimir O.
Published: September 2022

The search of a putative physiological electron acceptor for thiocyanate dehydrogenase (TcDH) newly discovered in the thiocyanate-oxidizing bacteria Thioalkalivibrio paradoxus revealed an unusually large, single-heme cytochrome c (CytC552), which was co-purified with TcDH from the periplasm. Recombinant CytC552, produced in Escherichia coli as a mature protein without a signal peptide, has spectral properties similar to the endogenous protein and serves as an in vitro electron acceptor in the TcDH-catalyzed reaction. The CytC552 structure determined by NMR spectroscopy reveals significant differences compared to those of the typical class I bacterial cytochromes c: a high solvent accessible surface area for the heme group and so-called “intrinsically disordered” nature of the histidine-rich N- and C-terminal regions. Comparison of the signal splitting in the heteronuclear NMR spectra of oxidized, reduced, and TcDH-bound CytC552 reveals the heme axial methionine fluxionality. The TcDH binding site on the CytC552 surface was mapped using NMR chemical shift perturbations. Putative TcDH-CytC552 complexes were reconstructed by the information-driven docking approach and used for the analysis of effective electron transfer pathways. The best pathway includes the electron hopping through His528 and Tyr164 of TcDH, and His83 of CytC552 to the heme group in accordance with pH-dependence of TcDH activity with CytC552.
Nephritis-Associated Plasmin Receptor (NAPlr): An Essential Inducer of C3-Dominant Glomerular Injury and a Potential Key Diagnostic Biomarker of Infection-Related Glomerulonephritis (IRGN)
Authors: Yoshizawa Nobuyuki , Yamada Muneharu , Fujino Masayuki , Oda Takashi
Published: September 2022

Nephritis-associated plasmin receptor (NAPlr) was originally isolated from the cytoplasmic fraction of group A Streptococci, and was found to be the same molecule as streptococcal glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and plasmin receptor (Plr) on the basis of nucleotide and amino acid sequence homology. Its main functions include GAPDH activity, plasmin-binding capacity, and direct activation of the complement alternative pathway (A-P). Plasmin trapped by deposited NAPlr triggers the degradation of extracellular matrix proteins, such as glomerular basement membranes and mesangial matrix, and the accumulation of macrophages and neutrophils, leading to the induction of plasmin-related endocapillary glomerular inflammation. Deposited NAPlr at glomerular endocapillary site directly activates the complement A-P, and the endocapillary release of complement-related anaphylatoxins, C3a and C5a, amplify the in situ endocapillary glomerular inflammation. Subsequently, circulating and in situ-formed immune complexes participate in the glomerular injury resulting in NAPlr-mediated glomerulonephritis. The disease framework of infection-related glomerulonephritis (IRGN) has been further expanded. GAPDH of various bacteria other than Streptococci have been found to react with anti-NAPlr antibodies and to possess plasmin-binding activities, allowing glomerular NAPlr and plasmin activity to be utilized as key biomarkers of IRGN.
Updated Confirmatory Diagnosis for Mucopolysaccharidoses in Taiwanese Infants and the Application of Gene Variants
Authors: Chuang Chih-Kuang , Tu Yuan-Rong , Lee Chung-Lin , Lo Yun-Ting , Chang Ya-Hui , Liu Mei-Ying , Liu Hsin-Yun , Chen Hsiao-Jan , Kao Shu-Min , Wang Li-Yun , Ho Huey-Jane , Lin Hsiang-Yu , Lin Shuan-Pei
Published: September 2022

Mucopolysaccharidosis (MPS) is a lysosomal storage disease caused by genetic defects that result in deficiency of one specific enzyme activity, consequently impairing the stepwise degradation of glycosaminoglycans (GAGs). Except for MPS II, the other types of MPS have autosomal recessive inheritance in which two copies of an abnormal allele must be present in order for the disease to develop. In this study, we present the status of variant alleles and biochemistry results found in infants suspected of having MPS I, II, IVA, and VI. A total of 324 suspected infants, including 12 for MPS I, 223 for MPS II, 72 for MPS IVA, and 17 for MPS VI, who were referred for MPS confirmation from newborn screening centers in Taiwan, were enrolled. In all of these infants, one specific enzyme activity in dried blood spot filter paper was lower than the cut-off value in the first blood sample, as well asin a second follow-up sample. The confirmatory methods used in this study included Sanger sequencing, next-generation sequencing, leukocyte enzyme fluorometric assay, and GAG-derived disaccharides in urine using tandem mass spectrometry assays. The results showed that five, nine, and six infants had MPS I, II, and IVA, respectively, and all of them were asymptomatic. Thus, a laboratory diagnosis is extremely important to confirm the diagnosis of MPS. The other infants with identified nucleotide variations and reductions in leukocyte enzyme activities were categorized as being highly suspected cases requiring long-term and intensive follow-up examinations. In summary, the final confirmation of MPS depends on the most powerful biomarkers found in urine, i.e., the quantification of GAG-derived disaccharides including dermatan sulfate, heparan sulfate, and keratan sulfate, and analysis of genetic variants can help predict outcomes and guide treatment.
The NHE3 Inhibitor Tenapanor Prevents Intestinal Obstructions in CFTR-Deleted Mice
Authors: Tan Xinjie , Kini Archana , Römermann Dorothee , Seidler Ursula
Published: September 2022

Mutations in the CFTR chloride channel result in intestinal obstructive episodes in cystic fibrosis (CF) patients and in CF animal models. In this study, we explored the possibility of reducing the frequency of obstructive episodes in cftr−/− mice through the oral application of a gut-selective NHE3 inhibitor tenapanor and searched for the underlying mechanisms involved. Sex- and age-matched cftr+/+ and cftr−/− mice were orally gavaged twice daily with 30 mg kg−1 tenapanor or vehicle for a period of 21 days. Body weight and stool water content was assessed daily and gastrointestinal transit time (GTT) once weekly. The mice were sacrificed when an intestinal obstruction was suspected or after 21 days, and stool and tissues were collected for further analysis. Twenty-one day tenapanor application resulted in a significant increase in stool water content and stool alkalinity and a significant decrease in GTT in cftr+/+ and cftr−/− mice. Tenapanor significantly reduced obstructive episodes to 8% compared to 46% in vehicle-treated cftr−/− mice and prevented mucosal inflammation. A decrease in cryptal hyperproliferation, mucus accumulation, and mucosal mast cell number was also observed in tenapanor- compared to vehicle-treated, unobstructed cftr−/− mice. Overall, oral tenapanor application prevented obstructive episodes in CFTR-deficient mice and was safe in cftr+/+ and cftr−/− mice. These results suggest that tenapanor may be a safe and affordable adjunctive therapy in cystic fibrosis patients to alleviate constipation and prevent recurrent DIOS.
Fabrication of Nanostructures on a Large-Area Substrate with a Minimized Stitch Error Using the Step-and-Repeat Nanoimprint Process
Authors: Ha Yeonjoo , Lim Hyungjun , Choi Hak-jong , Lee JaeJong
Published: September 2022

Nanoimprint lithography (NIL) is suitable for achieving high uniformity and mass production. However, in conventional NIL, a stamp suitable for the substrate size is required to increase the substrate size. To address this issue, we fabricated nanostructures on a large-area substrate using step-and-repeat NIL after making a small stamp. A stamp was produced using glass, and a nano-pillar pattern with a diameter of 600 nm, an interval of 400 nm, and a height of 270 nm was used during the experiment. The area of the pattern on the stamp was 10 mm × 10 mm, and the step-and-repeat process was performed 25 times to transfer the nanostructures to a 4-inch substrate. In addition, stitch gaps were created between the patterns, which could decrease the performance upon future application. To minimize this stitch gap, a high-precision glass scale was attached to the stamp feeder to precisely control the position and to minimize the step difference. Moreover, an experiment was conducted to minimize the stitch gap by adjusting the movement interval of the stamp, and the stitch spacing was minimized by moving the stamp position by 9.97 mm. This approach will facilitate the manufacturing of large-area substrates and other structures in the future.
First Principle Surface Analysis of YF3 and Isostructural HoF3
Authors: Anders Jennifer , Limberg Niklas , Paulus Beate
Published: September 2022

The trifluorides of the two high field strength elements yttrium and holmium are studied by periodic density functional theory. As a lanthanide, holmium also belongs to the group of rare earth elements (REE). Due to their equivalent geochemical behavior, both elements form a geochemical twin pair and consequently, yttrium is generally associated with the REE as REE+Y. Interestingly, it has been found that DFT/DFT+U describe bulk HoF3 best, when the 4f-electrons are excluded from the valence region. An extensive surface stability analysis of YF3 (PBE) and HoF3 (PBE+Ud/3 eV/4f-in-core) using two-dimensional surface models (slabs) is performed. All seven low-lying Miller indices surfaces are considered with all possible stoichiometric or substoichiometric terminations with a maximal fluorine-deficit of two. This leads to a scope of 24 terminations per compound. The resulting Wulff plots consists of seven surfaces with 5–26% abundance for YF3 and six surfaces with 6–34% for HoF3. The stoichiometric (010) surface is dominating in both compounds. However, subtle differences have been found between these two geochemical twins.
Energy-Dependent Particle Size Distribution Models for Multi-Disc Mill
Authors: Kruszelnicka Weronika , Opielak Marek , Ambrose Kingsly , Pukalskas Saugirdas , Tomporowski Andrzej , Walichnowska Patrycja
Published: September 2022

Comminution is important in the processing of biological materials, such as cereal grains, wood biomass, and food waste. The most popular biomaterial grinders are hammer and roller mills. However, the grinders with great potential in the processing of biomass are mills that use cutting, e.g., disc mills. When it comes to single-disc and multi-disc grinders, there are not many studies describing the relationships between energy, motion, material, and processing or describing the effect of grinding, meaning the size distribution of a product. The relationship between the energy and size reduction ratio of disc-type grinder designs has also not been sufficiently explored. The purpose of this paper was to develop models for the particle size distribution of the ground product in multi-disc mills depending on the variable process parameters, i.e., disc rotational velocity and, consequently, power consumption, and the relationship between the grinding energy and the shape of graining curves, which would help predict the product size reduction ratio for these machines. The experiment was performed using a five-disc mill, assuming the angular velocity of the grinder discs was variable. Power consumption, product particle size, and specific comminution energy were recorded during the tests. The Rosin–Rammler–Sperling–Bennet (RRSB) distribution curves were established for the ground samples, and the relationships between distribution coefficients and the average angular velocity of grinder discs, power consumption, and specific comminution energy were determined. The tests showed that the specific comminution energy increases as the size reduction ratio increases. It was also demonstrated that the RRSB distribution coefficients could be represented by the functions of angular velocities, power consumption, and specific comminution energy. The developed models will be a source of information for numerical modelling of comminution processes.
Hard Carbon Embedded with FeSiAl Flakes for Improved Microwave Absorption Properties
Authors: Sun Xiaogang , Liu Yi , Kuang Daitao , Lu Jun , Yang Junyi , Peng Xiaomin , Wu Anru
Published: September 2022

Carbon-based composites have been proven to be strong candidates for microwave absorbers in recent years. However, as an important member, magnetic hard carbon (HC)-based composites have rarely been studied in the field of microwave absorption. In this study, HC embedded with FeSiAl (FeSiAl@HC) was synthesized by pyrolyzing a mixture of FeSiAl flakes and phenolic resin (PR). The as-synthesized HC-FeSiAl exhibited a layered structure, and the detailed microstructures were modified by changing the mass ratio of FeSiAl flakes and PR. Thus, the as-synthesized HC-FeSiAl exhibited tunable magnetic properties, wealthy functional groups, excellent thermal stability, and enhanced microwave absorption properties. The optimal minimum reflection loss is lower up to −36.1 dB, and the effective absorption bandwidth is wider up to 11.7 GHz. These results indicated that HC-FeSiAl should be a strong candidate for practical applications of microwave absorption, which may provide new insight into the synthesis of magnetic HC-based composites.
Correlation between Nonlinear Optical Effects and Structural Features of Aurone-Based Methacrylic Polymeric Thin Films
Authors: Waszkowska Karolina , Krupka Anastasiia , Smokal Vitaliy , Kharchenko Oksana , Migalska-Zalas Anna , Frasinyuk Mykhaylo , Wielgosz Robert , Andrushchak Anatoliy , Sahraoui Bouchta
Published: September 2022

In this study, new photonics architectures and aurone-based methacrylic polymers were designed and synthesized for their optical and nonlinear optical properties. The studied polymeric thin films were deposited by spin coating method. SHG and THG effects were measured via Maker fringe technique in transmission mode and determined using theoretical models. Investigations involved the theoretical quantum chemical calculation of dipole moments, frontier molecular orbital HOMO and LUMO energies, and first (β) and second (γ) hyperpolarizabilities. We determined the impact of the substitution in the para position of the phenyl ring and at the dipole moment of the chromophore on the nonlinear optical properties of the investigated polymers. The presented theoretical and experimental studies provide important information with respect to the design of methacrylic-based polymeric thin film devices and supplement existing knowledge with respect to their nonlinear behaviour.
Transport and Electrochemical Properties of Li4Ti5O12-Li2TiO3 and Li4Ti5O12-TiO2 Composites
Authors: Kozlova Anna , Uvarov Nikolai , Ulihin Artem
Published: September 2022

The study demonstrates that the introduction of the electrochemically inactive dielectric additive Li2TiO3 to LTO results in a strong decrease in the grain boundary resistance of LTO-Li2TiO3 (LTC) composites at a low concentration of Li2TiO3. With the increase in the concentration of Li2TiO3 in LTC composites, the grain boundary resistance goes through a minimum and increases again due to the growth of the insulation layer of small Li2TiO3 particles around LTO grains. For LTO-TiO2 (LTT) composites, a similar effect was observed, albeit not as strong. It was found that LTC composites at low concentration of Li2TiO3 have unusually high charge–discharge capacity exceeding the theoretical value for pure LTO. This effect is likely to be caused by the occurrence of the electrochemical activity of Li2TiO3 in the vicinity of the interfaces between LTO and Li2TiO3. The increase in the capacity may be qualitatively described in terms of the model of two-phase composite in which there is the interface layer with a high capacity. Contrasting with LTC composites, in LTT composites, no capacity enhancement was observed, which was likely due to a noticeable difference in crystal structures of LTO and TiO2 preventing the formation of coherent interfaces.