bluuebunny/crossref_metadata_2025 huggingface dataset:
Muscle Advancement During Arthroscopic Rotator Cuff Repair as a Treatment Option for Irreparable Rotator Cuff Tears
Authors: Oh Sang Yun , Kim Yong Tae , Lee Kyung Jae , Kim Sae Hoon
Published: September 2024

Background:

The management of irreparable rotator cuff tears presents a surgical dilemma. However, supraspinatus muscle advancement (MA) could be used to convert irreparable to reparable tears without requiring a graft.

Purpose:

To compare the outcomes of patients with an irreparable tear who underwent rotator cuff repair with MA with those with a reparable large to massive cuff tear who underwent rotator cuff repair.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

We enrolled 62 patients who underwent rotator cuff repair for a large to massive tear between January 2020 and May 2022. Among them, 29 patients underwent an MA procedure due to an inability to repair despite releases (MA group), whereas the other 33 patients did not require the procedure (NMA group). At 1 year postoperatively, follow-up assessments including magnetic resonance imaging were performed to evaluate group outcomes.

Results:

Despite there being more revision surgery cases and a poorer potential for cuff healing in the MA group, the retear rates in the MA and NMA groups were similar (31.0% vs 21.2%, respectively; P = .401), as were clinical outcomes, including the visual analog scale for pain (2.9 vs 1.9; P = .076), University of California, Los Angeles (27.0 vs 29.1; P = .185), Constant (70.1 vs 74.9; P = .063), and American Shoulder and Elbow Surgeons (74.5 vs 81.8; P = .168) scores. Postoperative muscle power during forward elevation was weaker in the MA group than in the NMA group (35.1 vs 45.8 N; P = .052), but external rotation power was comparable (49.3 vs 59.0 N; P = .121). Progress in fatty degeneration of the supraspinatus was not significantly different in the 2 groups ( P = .43), although the MA group showed a nonsignificant decrease in supraspinatus atrophy ( P = .092) due to the lateral shift produced by the procedure.

Conclusion:

The MA procedure for irreparable tears produced outcomes comparable with reparable tears, offering a valuable perspective on the efficiency of the procedure. The comparable but relatively high retear rates emphasize the need for further studies to compare with other treatment options for irreparable tears.
Insurance Status, Language, and Access to Care for Pediatric Anterior Cruciate Ligament Injury
Authors: Park Anna L. , Singh Dhillon Pardeep , Pandya Nirav K.
Published: September 2024

Background:

Patients with public insurance (PUBs) face more difficulty obtaining orthopaedic appointments and have longer wait times than privately insured patients (PVTs). These delays are associated with greater injury severity at the time of surgery, which affects sports injuries such as anterior cruciate ligament tears where early surgical stabilization leads to better outcomes. Additionally, previous evidence showed that patients with limited English proficiency often must rely on informal translation services, such as family members or friends, to communicate with their orthopaedic surgeons, which may represent a disparity in the care provided.

Hypothesis:

It was hypothesized that PUBs would be less likely to obtain an appointment compared with PVTs and that most providers would not offer professional translation services to Spanish-speaking patients.

Study Design:

Cross-sectional study.

Methods:

The authors called 50 randomly selected orthopaedic surgeons’ offices in California specializing in sports medicine to request an appointment. Each office was called 4 times in random order for the hypothetical patient having either private or public insurance and speaking either Spanish or English.

Results:

The hypothetical PUB had significantly decreased access to an appointment (19% of offices offered an appointment) when compared to the PVT (73.8% offered an appointment). Independent private practice (IPP) offices were less likely to accept public insurance (13.3%) compared with offices at academic medical centers (57.1%). There was no difference in access to an appointment for the Spanish- versus English-speaking patient. Translation services were offered at 73.8% of the orthopaedic offices.

Conclusion:

Overall, the data illustrated disparities in access to pediatric orthopaedic care for PUBs compared to those with private insurance. Disparities were most prominent in IPP settings, which were less likely than academic offices to accept public insurance. Additionally, it was found that 73.8% of the offices the authors contacted offered Spanish translation services. Interventions should focus on increasing acceptance of public insurance and translation services in IPP settings. Future studies should expand this analysis to other languages and investigate the potential impacts of language on the quality of care provided.
Fight the Power? How Black Adults’ Racial Capital Associates With Their Political Activities
Authors: Gorman Quintin
Published: September 2024

Systemic racism explains historical and contemporary anti-black exclusion, violence, and exploitation in the United States. Yet, Black people routinely resist systemic racism’s effects. This study asks whether racial capital (i.e., Blacks’ belief in the significance of systemic racism) associates positively with political activities. It also attempts to replicate previous findings showing educational attainment directly predicts political activities. Finally, it asks whether educational attainment moderates the association between racial capital and political activities. Analyses of nationally representative data from the Outlook on Life Surveys, 2012, indicate racial capital associates positively with political activities. Further, educational attainment directly predicts political activities and seemingly attenuates racial capital’s positive association with political activities. However, racial capital associates positively with increased political activities among Black people with an associate degree or more.
Constructing a governmental vision of happiness: Insights from Greece
Authors: Katsinas Philipp , Soudias Dimitris
Published: September 2024

This paper analyzes how governments instrumentalize the concept of happiness for political ends. It argues that while happiness is primarily employed as an externally-oriented policy and discourse to attract tourists and desirable migrants, it is equally aimed at changing the expectations of the local population, including brain-drainers. We argue that in the case of Greece, happiness forms a governmental vision to brand the country anew after years of moralizing discourses of guilt, blame, and debt surrounding the financial crisis. First, we outline how the Greek government construes happiness as a commodified experience that the Greek population ought to generate for tourists and desirable migrants (‘live like a local’), but importantly also for itself (‘live like a tourist’). Second, this happiness vision seeks to both encourage the Greek population (in that we want to be happy), but also to discipline it (in that we need to be happy). Thirdly, to justify this vision, its key promoters conceive of a future that requires sanitizing the country’s past and present, camouflage its unpleasant and contentious aspects, and re-narrate it in positive terms. Curiously, however, this is less about envisioning a better future after years of crisis, than about asking the Greek population to be satisfied with the status quo. This way, the happiness vision is an attempt to substitute the unfulfilled promises of the capitalist imaginary vis-à-vis opportunity, upward social mobility, and overabundance, where happiness arises not by overcoming the precarizing realities of inequality, but from having a positive attitude in navigating them.
Interpersonal Violence and Mental Health, Drug use, and Treatment Utilization among Patients with Co-Occurring Opioid use and Mental Health Disorders
Authors: Hindmarch Grace , Meredith Lisa S. , McCullough Colleen M. , Griffin Beth Ann , Watkins Katherine E.
Published: September 2024

Interpersonal violence is a common type of trauma experienced by people with opioid use disorder (OUD), especially for people with co-occurring OUD and mental illness (COD). However, little is known about demographic and clinical characteristics of individuals with COD who have experienced an interpersonal violence traumatic event compared to those who have experienced a non-violent trauma, and how experiences of interpersonal violence are associated with treatment utilization. Data presented are from a randomized clinical trial testing collaborative care for COD in primary care. Of the 797 patients enrolled in the larger study, 733 (92%) were included in this analysis because they reported a traumatic event. In this sample, 301 (41%) participants experienced a traumatic event involving interpersonal violence. Participants who experienced interpersonal violence were more likely to be younger and female. Among the 301 people who experienced interpersonal violence, 30% experienced child sexual abuse, 23% experienced physical violence, 19% experienced domestic violence, and 28% experienced sexual assault. Those who experienced physical violence were significantly less likely to be female (28.6% vs 74.2% to 88.2% in other groups). Those who reported domestic violence had significantly fewer days of drug use (4.1 days vs 9.0 to 11.5 in the other groups) and lower opioid use severity scores (mean = 13.0 vs 16.6 to 19.5 in the other groups). Multivariable regression results examining the associations between interpersonal violence experiences on treatment utilization revealed no statistically significant differences. Rates of receipt were high for medication for opioid use disorder (∼80%) in this sample while rates of mental health counseling were around 35% and rates of receiving mental health medication around 48%. These findings make an important contribution to understanding the associations between patient characteristics and traumatic experiences, and receipt of treatment for OUD and mental health problems among a sample of patients with COD.

Clinical trial registration

clinicalTrials.gov ID: NCT04559893
Illuminating radical spatial imaginaries: Counter-mapping urban environmental justice struggles for the city yet to come
Authors: Matheney Austin , Anguelovski Isabelle , Kotsila Panagiota , Sekulova Filka , Oscilowicz Emilia
Published: September 2024

Urban greening is not a neutral intervention on the social fabric. Critical analyses of the green city often concentrate on greening's top-down, hegemonic, and large-scale manifestations while neglecting the role of social movements. Aiming to address this gap, we ground discussions of “urbanized nature” within discussions of radical spatial imaginaries, concentrating on their performativity. We propose “counter-mapping” as an engaged methodology, developed with local activists, to illuminate radical green imaginaries. Examining 13 environmental justice struggles in Barcelona, we find place imaginaries to be tied to preserving unique sites, linking historical trauma to fears of future spatial fixes, and projecting inclusive, liveable neighborhoods as ideal futures. This study underscores the importance of comprehending how resistance to the prevailing “green-paradox” manifests in local contexts. Through this exercise, we might help make clearer the axes of common/divergent imaginaries and forge alliances, dialogues, and reflection among local movements and decision makers in Barcelona and beyond.
Behaviours and psychological symptoms of childhood dementia: two cases of psychosocial interventions
Authors: Atee Mustafa , Whiteman Ineka , Lloyd Rebecca , Morris Thomas
Published: September 2024

Childhood dementias are a group of rare, fatal neurodegenerative disorders, characterised by global cognitive decline, loss of previously acquired developmental skills and behaviours and psychological symptoms of dementia (BPSD). Batten disease, or neuronal ceroid lipofuscinosis, and Sanfilippo syndrome, or mucopolysaccharidosis type III, are two of the more common forms of childhood dementia disorders worldwide. While psychosocial interventions are the best available therapeutic approach for BPSD management in adult-onset dementia, there is very limited literature or clinical experience in the context of childhood dementia. To address this gap, we conducted a descriptive case analysis of BPSD profiles, associated contributing factors and targeted psychosocial interventions in two cases with childhood dementia disorders (Sanfilippo syndrome and CLN3 (juvenile onset) Batten disease) who were referred to Dementia Support Australia, a national dementia behaviour support service in Australia. Primary BPSD identified in these disorders included physical and verbal aggression and irritability/lability. In these cases, contributing factors to the development of BPSD were not monolithic, encompassing pain, caregiver’s approach and over or under-stimulation. Improvement in BPSD were observed using the Neuropsychiatric Inventory-Quesionnaire and globally noted as per the qualitative feedback reported by family and caregivers. Person-centred, multimodal psychosocial interventions were recognised as effective therapies in resolving BPSD in these cases. In conclusion, the case studies described the nature and presentation of BPSD in two common forms of childhood dementia and demonstrated the potential benefits of person-centred psychosocial interventions (delivered through national dementia-specific support programs) in alleviating BPSD such as irritability and aggression in these disorders.
Community engagement in a seaside town: evaluation of Good Grief Weston festival
Authors: Robb James , Clabburn Olly , Bamford Alison , Matthews Fiona , Lee Karen , Toulcher Lin , Maxwell Polly , Thomas-Bennett Nina , Hare Rachel , Dawson Lesel , Malpass Alice , Selman Lucy E.
Published: September 2024

Background:

Festivals play an important role in improving death and grief literacy, enabling members of the public to engage with these often-sensitive topics. Good Grief Weston festival was co-designed and delivered with the community in Weston-super-Mare, a coastal town in South-West England with high levels of socioeconomic disadvantage but rich community assets. It was held in person over 8 days in May 2023.

Objectives:

To evaluate the reach and impact of Good Grief Weston festival and gather data to inform future festivals.

Design:

Mixed methods evaluation (survey and focus groups).

Methods:

Online and paper surveys assessing participants’ characteristics and experiences were administrated during and after the festival. Survey participants who indicated their willingness to participate were invited to attend a focus group. Focus groups were recorded, transcribed and analysed using thematic analysis. Data were collected by trained community co-researchers.

Results:

Approximately 3000 people attended the festival. Of 204 completed surveys, 64.5% were from women, age range ⩽15 to ⩾75 years; 88.2% identified as White; 14.9% deaf, disabled/with a chronic condition; 18.9% neurodivergent; 9.0% gay, bisexual or queer. Festival participants were entertained (70.9%), inspired (68.5%), felt part of a like-minded community (54.3%), talked to someone new (49.2%), learnt about grief/bereavement (34.3%), shared or expressed experiences (30.3%) and found out about local support (19.7%). 71.3% reported that they felt more confident talking about grief after attending. Median experience rating was 5 (IQR 0; possible range 1 = poor to 5 = excellent). In free-text comments, participants expressed appreciation for the festival and described benefits in attending. Two focus groups were conducted ( n = 8 participants, all women), lasting c.1.5 h. Focus groups added rich descriptions of the festival’s value, and data to inform the next festival.

Conclusion:

Findings suggest festivals of this nature can play a central role in a public health approach.
A roadmap for SHANK3-related Epilepsy Research: recommendations from the 2023 strategic planning workshop
Authors: Savage Margaret C. , Bliss Geraldine , Buxbaum Joseph D. , Farrell Jordan S. , Levin April R. , Srivastava Siddharth , Berry-Kravis Elizabeth , Holder J. Lloyd , Sahin Mustafa
Published: September 2024

On September 27, 2023, the CureSHANK nonprofit foundation sponsored a conference in Boston, Massachusetts, to identify gaps in knowledge surrounding SHANK3-related epilepsy with the goal of determining future research priorities and recommendations. In addition to patient families and members of the CureSHANK community, participants in the conference included a broad cross-section of preclinical and clinical researchers and clinicians with expertise in SHANK3-related epilepsy as well as representatives from the pharmaceutical industry. Here we summarize the outcomes from comprehensive premeeting deliberations and the final conference recommendations, including (1) gaps in knowledge related to clinical science, (2) gaps in knowledge related to preclinical science, and (3) research priorities moving forward.
Diabetes Self-Care Behaviors in Singapore and Their Associations With Patients’ Characteristics and Health Literacy
Authors: Koh Yen Sin , AshaRani P. V. , Devi Fiona , Roystonn Kumarasan , Wang Peizhi , Abdin Edimansyah , Sum Chee Fang , Lee Eng Sing , Chong Siow Ann , Subramaniam Mythily
Published: September 2024

Purpose

The purpose of this study was to examine the relationship between self-management behaviors (eg, healthy eating, being active, medication taking, glucose monitoring, feet check), sociodemographic factors, disease-related characteristics, and health literacy among patients with type 2 diabetes in Singapore.

Methods

Data were analyzed from a nationwide survey conducted between 2019 and 2020 (n = 387). Self-management behaviors were assessed using the Dietary Approaches to Stop Hypertension questionnaire, the Global Physical Activity Questionnaire, and a diabetes care questionnaire. A linear regression model was generated to examine the association of healthy eating with the variables of interest (sociodemographic factors, disease-related characteristics, and health literacy), and logistic regression models were generated to investigate the significant correlates of the remaining self-care behaviors.

Results

Regression models showed that the 5 self-care behaviors have different correlates. Nonetheless, compared to individuals aged 50 to 64 years, those aged 65 years and above were less likely to be active, adhere to their medication prescription, and check their feet. Individuals with a higher number of diabetes-related complications were less likely to be sufficiently active but more likely to monitor their glucose level and check their feet. Moreover, individuals with poor health literacy were more likely to eat healthily and be sufficiently active.

Conclusions

Programs related to self-care behaviors can be tailored to specific demographics to improve their uptake in the population. Furthermore, encouraging comprehensive self-care behaviors in those aged 65 years and above is crucial for effective diabetes management.
Spatiotemporal evolution in achieving the Sustainable Development Goals on the Qinghai–Tibet Plateau in 2000–2020
Authors: Dai Erfu , Li Xian , Jia Lizhi , Zhao Zhongxu , Zhao Lin
Published: September 2024

Assessing the sustainable development level of the Qinghai–Tibet Plateau (QTP) is of great significance for China’s sustainable development. With rich natural resources, a fragile ecological environment, and a relatively low level of economic development, the QTP is typical of regions in China where conflict between socio-economic development and environmental protection is prominent. However, there is a lack of assessment of spatiotemporal progress in achieving the Sustainable Development Goals (SDGs) on the QTP. To address this gap in knowledge, cluster analysis was used to quantitatively assess spatiotemporal changes in SDG indicators and sustainable development levels in different counties of the QTP from 2000 to 2020. The results show that the sustainable development level of the QTP is increasing; the growth rate of the sustainable development in 2010–2020 was three times that of 2000–2010. The level of sustainable development in the eastern region is significantly higher than that in the western region. However, while the level of sustainable environmental development is high, the levels of sustainable social and economic development remain low. Certain factors, including precipitation, slope, population density, and cultivated area, are strongly positively correlated with the sustainable development of the QTP. The findings of our study can serve as a valuable reference for other jurisdictions seeking to evaluate their evolution towards the SDGs.
Streptomyces avermitilis MICNEMA2022: a new biorational strain for producing abamectin as an integrated nematode management agent
Authors: Radwan Wafaa H. , Abdelhafez Ahmed A. M. , Mahgoub Ahmed E. , Zayed Mona S.
Published: September 2024

Background

Abamectin (ABA) is considered a powerful insecticidal and anthelmintic agent. It is an intracellular product of Streptomyces avermitilis; is synthesized through complicated pathways and can then be extracted from mycelial by methanol extraction. ABA serves as a biological control substance against the root-knot nematode Meloidogyne incognita. This investigation is intended to reach a new strain of S. avermitilis capable of producing ABA effectively.

Results

Among the sixty actinobacterial isolates, Streptomyces St.53 isolate was chosen for its superior nematicidal effectiveness. The mycelial-methanol extract of isolate St.53 exhibited a maximum in vitro mortality of 100% in one day. In the greenhouse experiment, the mycelial-methanol extract demonstrated, for the second-stage juveniles (J2s), 75.69% nematode reduction and 0.84 reproduction rate (Rr) while for the second-stage juveniles (J2s), the culture suspension demonstrated 75.38% nematode reduction and 0.80 reproduction rate (Rr). Molecular identification for St.53 was performed using 16 S rRNA gene analysis and recorded in NCBI Genbank as S. avermitilis MICNEMA2022 with accession number (OP108264.1). LC-MS was utilized to detect and identify abamectin in extracts while HPLC analysis was carried out for quantitative determination. Both abamectin B1a and abamectin B1b were produced and detected at retention times of 4.572 and 3.890 min respectively.

Conclusion

Streptomyces avermitilis MICNEMA2022 proved to be an effective source for producing abamectin as a biorational agent for integrated nematode management.
Primary care team characteristics associated with video use: a retrospective national study at the Veterans Health Administration
Authors: Der-Martirosian Claudia , Yoo Caroline K. , Steers W. Neil , Hou Cynthia G. , Chu Karen , Ferguson Jacqueline , Carter Maia , Heyworth Leonie , Leung Lucinda B.
Published: September 2024

Objective

To examine primary care (PC) team members’ characteristics associated with video use at the Veterans Health Administration (VA).

Methods

VA electronic data were used to identify PC team characteristics associated with any video-based PC visit, during the three-year study period (3/15/2019-3/15/2022). Multilevel mixed-effects logistic regression models on repeated yearly observations were used, adjusting for patient- and healthcare system-level characteristics, and study year. We included five PC team categories: 1.PC providers (PCP), which includes physicians, nurse practitioners, physician assistants, 2.Nurses (RN/LVN/LPN/other nurses), 3.Mental health (MH) specialists, 4.Social workers (SW), and 5.Clinical pharmacists (PharmD).

Population

54,494 PC care team members nationwide (61,728,154 PC visits; 4,916,960 patients), including 14,422 PCPs, 30,273 nurses, 2,721 MH specialists, 4,065 SWs, and 3,013 PharmDs.

Results

The mean age was 46.1(SD = 11.3) years; 77.1% were women. Percent of video use among PC team members varied from 24 to 84%. In fully adjusted models, older clinicians were more likely to use video compared to the youngest age group (18–29 years old) (example: 50–59 age group: OR = 1.12,95%CI:1.07–1.18). Women were more likely to use video (OR = 1.18, 95%CI:1.14–1.22) compared to men. MH specialists (OR = 7.87,95%CI:7.32–8.46), PharmDs (OR = 1.16,95%CI:1.09–1.25), and SWs (OR = 1.51,95%CI:1.41–1.61) were more likely, whereas nurses (OR = 0.65,95%CI:0.62–0.67) were less likely to use video compared to PCPs.

Conclusions

This study highlights more video use among MH specialists, SWs, and PharmDs, and less video use among nurses compared to PCPs. Older and women clinicians, regardless of their role, used more video. This study helps to inform the care coordination of video-based delivery among interdisciplinary PC team members.
Unilateral biportal endoscopic discectomy via translaminar approach for highly upward-migrated lumbar disc herniation: a technical note and preliminary treatment outcomes
Authors: Chen Wein-Chin , Wang Wei-Ting , Pao Jwo-Luen
Published: September 2024

Study Design

A technical note and retrospective case series.

Objective

Highly upward-migrated lumbar disc herniation (LDH) is challenging due to its problematic access and incomplete removal. The most used interlaminar approach may cause extensive bony destruction. We developed a novel translaminar approach using the unilateral portal endoscopic (UBE) technique, emphasizing effective neural decompression, and preserving the facet joint’s integrity.

Methods

This retrospective study included six patients receiving UBE translaminar discectomy for highly upward-migrated LDHs from May 2019 to June 2021. The migrated disc was removed through a small keyhole on the lamina of the cranial vertebra. The treatment results were evaluated by operation time, hospital stays, complications, visual analog scale (VAS), Oswestry Disability Index (ODI), Japanese Orthopaedic Association (JOA) score, and modified MacNab criteria.

Results

The mean pre-operative VAS for back pain (5.0 ± 4.9), VAS for leg pain (9.2 ± 1.0), JOA score (10.7 ± 6.6), and ODI (75.7 ± 25.3) were significantly improved to 0.3 ± 0.5, 1.2 ± 1.5, 27.3 ± 1.8, 5.0 ± 11.3 respectively at the final follow-up. Five patients had excellent, and one patient had good outcomes according to the Modified MacNab criteria. The hospital stay was 2.7 ± 0.5 days. No complication was recorded. The MRI follow-up showed complete disc removal, except for one patient with an asymptomatic residual disc.

Conclusions

UBE translaminar discectomy is a safe and effective minimally invasive procedure for highly upward-migrated LDH with satisfactory treatment outcomes and nearly 100% facet joint preservation.
Genetic profiling and diagnostic strategies for patients with ectodermal dysplasias in Korea
Authors: Kim Man Jin , Lee Jee-Soo , Chae Seung Won , Cho Sung Im , Moon Jangsup , Ko Jung Min , Chae Jong-Hee , Seong Moon-Woo
Published: September 2024

Background

Ectodermal dysplasia (ED) is a rare genetic disorder that affects structures derived from the ectodermal germ layer.

Results

In this study, we analyzed the genetic profiles of 27 Korean patients with ED. Whole exome sequencing (WES) was performed on 23 patients, and targeted panel sequencing was conducted on the remaining 4 patients. Among the patients in the cohort, 74.1% (20/27) tested positive for ED. Of these positive cases, EDA and EDAR mutations were found in 80% (16/20). Notably, 23.1% (3/13) of EDA-positive cases exhibited copy number variations. Among the 23 patients who underwent WES, we conducted a virtual panel analysis of eight well-known genes, resulting in diagnoses for 56.5% (13/23) of the cases. Additionally, further analysis of approximately 5,000 OMIM genes identified four more cases, increasing the overall positivity rate by approximately 17%. These findings underscore the potential of WES for improving the diagnostic yield of ED. Remarkably, 94.1% of the patients manifesting the complete triad of ED symptoms (hair/skin/dental) displayed detectable EDA/EDAR mutations. In contrast, none of the 7 patients without these three symptoms exhibited EDA/EDAR mutations.

Conclusions

When conducting molecular diagnostics for ED, opting for targeted sequencing of EDA/EDAR mutations is advisable for cases with classical symptoms, while WES is deemed an effective strategy for cases in which these symptoms are absent.
Evaluation of the cytotoxic activity of chemically characterized propolis originating from different geographic regions and vitamin D co-supplementation against human ovarian cancer cells
Authors: Ali Eman , Helmy Maged W. , Radwan Eman H. , Abdul Aziz Karoline K. , El-Wahed Aida A. Abd , El-Samad Lamia M. , El Wakil Abeer
Published: September 2024

Ovarian cancer is the second most common and lethal gynecologic malignancy. Among natural product-based therapy, the honeybee products, particularly propolis, serve a valuable source contributing directly to human nutrition and health.

In the present study, we determined the chemical composition of different types of propolis originating from Egypt, Germany and France using liquid chromatography-tandem mass spectrometry. The compounds identified belong to different metabolite classes, including flavonoids, cinnamic acid, chalcones, terpenoids, phenolic lipids, stilbenes, phenolic compounds, carbohydrates, vitamins, coumarins, polyprenylated benzophenone, benzoic acids, fatty acid methyl ester, and coumaric acid, and their derivatives. The most active extract is from France then Egypt and Germany.

Afterwards, we treated the human ovarian cancer cells, OVCAR4, with different concentrations (1–400 μg/mL) of variable propolis types supplemented or not with vitamin D (0.0015–0.15 μg/mL) in order to evaluate the efficacy and the cytotoxic activities of our local P as compared to other types collected from different geographic regions. Importantly, the combinatorial treatment of OVCAR4 cancer cells with propolis and vitamin D in the same concentration ranges resulted in enhanced cell viability inhibition. Furthermore, such co-supplementation with vitamin D inhibits predominately the proliferative activity of cell population with the French propolis type as manifested by Ki67 expression, while it reduces considerably its expression, particularly with the German type, followed by the Egyptian one.

Nowadays, scientists are interested by natural products which have risen to the forefront of drug discovery. Chemically characterized propolis showing cell viability inhibition and antiproliferative potential seems a valuable extract for further consideration as anti-carcinogenic agent.

Graphical abstract
A silent strain: the unseen burden of acute respiratory infections in children
Authors: Boracchini Riccardo , Canova Benedetta , Ferrara Pietro , Cantarutti Luigi , Giaquinto Carlo , Di Chiara Costanza , Cantarutti Anna
Published: September 2024

The significant impact of acute respiratory tract infections on healthcare systems is well-documented, given their contribution to emergency department admissions, hospitalizations, and increased use of antibiotics and other medications. However, further research is needed to understand the burden of acute respiratory tract infections in pediatric community care to develop effective public health interventions and improve child health outcomes. Real-world data were retrieved from Pedianet, an Italian network of over 200 family pediatricians. Acute respiratory tract infection visits were identified and analysed using an infection-duration algorithm to extract individual cases. The number of follow-up visits per 100 cases was calculated to assess the burden on the Italian National Health Service. Comparisons were made overall and stratified by type of acute respiratory tract infections and epidemiological season. A total of 1,402,953 acute respiratory infections-related visits were recorded, with an overall rate of 12 visits per 100 cases. Upper respiratory tract infections had an average of 9 visits per 100 cases. Lower respiratory tract infections exhibited a higher burden, with 29 visits per 100 cases. Pneumonia showed a declining trend in the pre-pandemic era (62 to 48 visits) but rebounded in the post-COVID-19 years (32 to 42 visits). This study underscores the importance of monitoring and managing acute respiratory infections, especially lower respiratory tract infections, in pediatric care.
Using online methods to recruit participants into mental health clinical trials: considerations and recommendations from the RE-MIND study
Authors: Iflaifel Mais , Hall Charlotte L. , Green Heidi R. , Willis Andrew , Rennick-Egglestone Stefan , Juszczak Edmund , Townsend Mark , Martin Jennifer , Sprange Kirsty
Published: September 2024

Background

Ensuring diversity in clinical trials can be a challenge, which may be exacerbated when recruiting vulnerable populations, such as participants with mental health illness. As recruitment continues to be the major cause of trial delays, researchers are turning to online recruitment strategies, e.g. social media, to reach a wider population and reduce recruitment time and costs. There is mixed evidence for the use of online recruitment strategies; therefore, the REcruitment in Mental health trials: broadening the ‘net’, opportunities for INclusivity through online methoDs (RE-MIND) study aimed to identify evidence and provide guidance for use of online strategies in recruitment to mental health trials, with a focus on whether online strategies can enhance inclusivity. This commentary, as part of the RE-MIND study, focusses on providing recommendations for recruitment strategy selection in future research with the aim to improve trial efficiency.

A mixed-methods approach was employed involving three work packages: (I) an evidence review of a cohort of 97 recently published randomised controlled trials/feasibility or pilot studies in mental health to assess the impact of online versus offline recruitment; (II) a qualitative study investigating the experiences of n = 23 key stakeholders on use of an online recruitment approach in mental health clinical trials; (III) combining the results of WP1 and WP2 to produce recommendations on the use of an online recruitment strategy in mental health clinical trials. The findings from WP1 and 2 have been published elsewhere; this commentary represents the results of the third work package.

Conclusion

For external validity, clinical trial participants should reflect the populations that will ultimately receive the interventions being tested, if proven effective. To guide researchers on their options for inclusive recruitment strategies, we have developed a list of considerations and practical recommendations on how to maximise the use of online recruitment methods.
The microglial P2Y6 receptor as a therapeutic target for neurodegenerative diseases
Authors: Dundee Jacob M. , Brown Guy C.
Published: September 2024

Neurodegenerative diseases are associated with chronic neuroinflammation in the brain, which can result in microglial phagocytosis of live synapses and neurons that may contribute to cognitive deficits and neuronal loss. The microglial P2Y6 receptor (P2Y6R) is a G-protein coupled receptor, which stimulates microglial phagocytosis when activated by extracellular uridine diphosphate, released by stressed neurons. Knockout or inhibition of P2Y6R can prevent neuronal loss in mouse models of Alzheimer’s disease (AD), Parkinson’s disease, epilepsy, neuroinflammation and aging, and prevent cognitive deficits in models of AD, epilepsy and aging. This review summarises the known roles of P2Y6R in the physiology and pathology of the brain, and its potential as a therapeutic target to prevent neurodegeneration and other brain pathologies.
Enhancing efficiency in a-Si:H/μc-Si micromorph tandem solar cells through advanced light-trapping techniques using ARC, TRJ, and DBR
Authors: Khosroabadi Saeed , Eghbali Ramisa , Shokouhmand Anis
Published: September 2024

In this study, the performance of a-Si:H/μc-Si:H tandem solar cells was comprehensively assessed through two-dimensional numerical simulations. Our work involved optimizing the layer thicknesses and exploring advanced light-trapping techniques to enhance photogenerated current in both sub-cells. To reduce surface reflections on the top cell, we proposed a two-layer antireflection coating, composed of SiO2/Si3N4. Additionally, we implemented a 1D photonic crystal as a broadband back reflector within the solar cell. In order to balance the current density between the sub-cells and prevent carrier accumulation at the interface, we introduced a tunnel recombination junction (TRJ). This TRJ consisted of n-μc-Si:H/p-μc-Si:H layers with a thickness of 10 nm. Under global AM 1.5G conditions, our proposed cell structure exhibited impressive electrical characteristics, including an open-circuit voltage of 1.38 V, a short-circuit current density of 12.51 mA/cm2, and a fill factor of 80.82%. These attributes culminated in a remarkable total area conversion efficiency of 14%.
Ethical framework and inclusivity: research mechanics of difficult-to-reach migrants in civil military context
Authors: Ullah A. K. M. Ahsan
Published: September 2024

In the midst of humanitarian crises, understanding and helping hard-to-reach migrant communities, is a major challenge for researchers. This article addresses the ethical framework and dimensions of inclusivity associated with researching such populations in civilian-military setting. With the overarching aim of improving methodological discourse, this study seeks to understand the mechanisms involved in working with crisis-affected migrants who are often marginalised and geographically isolated. The significance of this research lies in its multi-layered approach to understanding the ethical considerations and inclusivity that are essential to conducting research in crisis situations. By examining existing theories in the field, including participatory research methods, ethnographic studies and community-based research frameworks, this study critically evaluates their applicability in the context of crisis-affected migrant communities. This research contributes to methodological approaches by proposing innovative strategies that emphasise ethical integrity, cultural sensitivity and inclusivity. It argues for the development of collaborative and culturally sensitive research practises that enable migrant communities to actively participate in the research process and ensure that their voices are heard, and their perspectives accurately represented. This study contributes to existing knowledge by illuminating the complexities of researching hard-to-reach migrant communities in civil military setting. This study provides researchers and practitioners with the necessary tools and perspectives required for engaging with crisis-affected migrant communities in a manner that is both ethical, respectful, and impactful.
The role of transthoracic echocardiography in evaluating right ventricular parameters in chronic obstructive pulmonary disease
Authors: Abdelalim Mohamed Ahmed Fouad , Khalil Mohamed A. , Sharshr Ragia Samir , Abdelzaher Ayman Hassan
Published: September 2024

Background

Cardiac symptoms are a characteristic feature of chronic obstructive pulmonary disease (COPD), a disorder with lately achieved recognition as a systemic illness according to established standards. The research’s objective was aimed at investigating the transthoracic echocardiography role in assessing right ventricular (RV) dysfunction among individuals diagnosed with COPD, as well as to examine the potential correlation between blood pro B-type natriuretic peptide (BNP) concentrations + and this diagnostic procedure.

Methods

Arterial blood gasses, spirometry, echocardiography, and serum pro BNP were done for 80 COPD patients and 20 healthy smokers, and some echocardiographic parameters were correlated with degree of airflow limitation.

Results

77.5% of COPD cases exhibited pulmonary hypertension (PHTN), and on classifying our COPD patients, it was found that 65% of patients in group II had PHTN and 90% of patients in group III had PHTN. Systolic pulmonary artery pressure (SPAP), mean pulmonary arterial pressure (MPAP), right ventricular mid diameter (RVMD), right ventricular basal diameter (RVBD), right ventricular longitudinal diameter (RVLD), right ventricular outflow tract (RVOT) above pulmonary and aortic valve, and tricuspid annular plane systolic excursion (TAPSE) along with RVEF exhibited a significant variance among all groups. A negative correlation was observed among forced expiratory volume (FEV) 1% and (RVMD as well as RVBD) and between Pro BNP and (TAPSE and RVEF).

Conclusions

PHTN was found in 77.5% of COPD patients and is negatively correlated with FEV1%. Serum pro BNP level exhibits a negative correlation with FEV1% in stable COPD patients.
A hierarchical frequency stability control strategy for distributed energy resources based on ADMM
Authors: Lan Guofeng , Xiao Jun , Zhao Wen , Li Wei , Zhao Yankai , Liu Yuchao , Yan Licheng , Zhao Lijie
Published: September 2024

With the large-scale distributed energy resources (DERs) interfaced by power electronic converters connected to the power grid, the traditional synchronous generation is gradually replaced, and the inertia and primary frequency regulation capability of the power system is weakened, which seriously threatens the frequency security of the power grid. In view of the above problems, a hierarchical control strategy by coordinating the distributed wind turbine (WT) and photovoltaic (PV) is presented to improve the transient frequency (TF) and steady-state frequency (SSF). For the system control level, the optimal frequency regulation coefficients of multiple WTs and PVs clustering systems are determined by the optimal control principle with minimum frequency regulation energy, while considering the constraints of TF and SSF. For the WT/PV control level, the frequency regulation coefficients of WTs and PVs are solved by the model predictive controller (MPC) and alternating direction method of multipliers (ADMM), with the minimum control cost of WT and PV. It achieves the optimal allocation of frequency regulation power and improves the computing efficiency. Finally, the validity of the proposed method is tested in the modified IEEE 10-machine 39-bus system.
Predictors of Cardiovascular Disease in Individuals With Dysbetalipoproteinemia: A Prospective Study in the UK Biobank
Authors: Paquette Martine , Trinder Mark , Guay Simon-Pierre , Brunham Liam R , Baass Alexis
Published: September 2024

Background

Dysbetalipoproteinemia (DBL) is a disorder of remnant cholesterol metabolism associated with a severe risk of atherosclerotic cardiovascular disease (ASCVD).

Objective

The objective of this study was to investigate the univariate and multivariate predictors of ASCVD in individuals with DBL.

Methods

Data from 2699 individuals with ɛ2/ɛ2 genotypes from the UK Biobank were included in this study. DBL was defined as having an ɛ2ɛ2 genotype with evidence of dyslipidemia, defined as total cholesterol ≥ 200 mg/dL (5.2 mmol/L) and triglyceride ≥ 175 mg/dL (2.0 mmol/L) or lipid-lowering therapy use (n = 964).

Results

Age, hypertension, waist circumference, and a polygenic risk score for coronary artery disease (PRSCAD) were independent predictors of ASCVD among individuals with DBL. Cumulative ASCVD-free survival was lower in the ɛ2/ɛ2 DBL group (84%) compared to the ɛ2/ɛ2 non-DBL group (94%) (P < .0001) and for DBL individuals with a PRSCAD ≥ median (79%) compared to those with a PRSCAD < median (89%) (P = .001).

Conclusion

We show in a large prospective cohort that a PRSCAD predicts the ASCVD risk among individuals with DBL. The findings of the present study highlight the need for better risk stratification in ɛ2/ɛ2 carriers to identify high-risk individuals who would need aggressive cardiovascular management despite their low apolipoprotein B value.
RNA Splicing Events in Circulation Distinguish Individuals With and Without New-onset Type 1 Diabetes
Authors: Webb-Robertson Bobbie-Jo M , Wu Wenting , Flores Javier E , Bramer Lisa M , Syed Farooq , Tersey Sarah A , May Sarah C , Sims Emily K , Evans-Molina Carmella , Mirmira Raghavendra G
Published: September 2024

Context

Alterations in RNA splicing may influence protein isoform diversity that contributes to or reflects the pathophysiology of certain diseases. Whereas specific RNA splicing events in pancreatic islets have been investigated in models of inflammation in vitro, how RNA splicing in the circulation correlates with or is reflective of type 1 diabetes (T1D) disease pathophysiology in humans remains unexplored.

Objective

To use machine learning to investigate if alternative RNA splicing events differ between individuals with and without new-onset T1D and to determine if these splicing events provide insight into T1D pathophysiology.

Methods

RNA deep sequencing was performed on whole blood samples from 2 independent cohorts: a training cohort consisting of 12 individuals with new-onset T1D and 12 age- and sex-matched nondiabetic controls and a validation cohort of the same size and demographics. Machine learning analysis was used to identify specific isoforms that could distinguish individuals with T1D from controls.

Results

Distinct patterns of RNA splicing differentiated participants with T1D from unaffected controls. Notably, certain splicing events, particularly involving retained introns, showed significant association with T1D. Machine learning analysis using these splicing events as features from the training cohort demonstrated high accuracy in distinguishing between T1D subjects and controls in the validation cohort. Gene Ontology pathway enrichment analysis of the retained intron category showed evidence for a systemic viral response in T1D subjects.

Conclusion

Alternative RNA splicing events in whole blood are significantly enriched in individuals with new-onset T1D and can effectively distinguish these individuals from unaffected controls. Our findings also suggest that RNA splicing profiles offer the potential to provide insights into disease pathogenesis.