bluuebunny/crossref_metadata_2025 huggingface dataset:
Embracing Nostalgia: The Irresistible Allure of Vintage Treasures Over Brand-New Alternatives
Authors: Wu Wanzhou
Published: December 2023

The obsession with vintage treasures over modern alternatives has spurred interest in the ever-changing landscape of consumer tastes, leading to a request for more research. This trend, which is founded on the appeal of nostalgia, crosses generations and countries, pushing people to embrace relics from the past in the face of rapid technological progress. This research delves into the intricate relationship between consumer behavior and nostalgia, providing light on the seductive pull of old goods and their market significance. The study methodology entails a qualitative examination via a literature review, with the goal of bridging gaps in academic understanding. Nostalgia amplifies this allure by forging emotional links between people and objects from the past. Marketing makes powerful connections with customers by advertising vintage objects as portals to personal and collective memories. Successful techniques emphasize the passage of goods through generations, produce limited-edition collections and cooperate with celebrities. However, an ethical quandary arises when marketers use emotions for economic advantage. It is critical to strike a balance between authenticity and manipulation. The study identifies gaps in the literature, indicating the need for further investigation. Lastly, the ongoing attractiveness of nostalgia-driven vintage treasures demonstrates the inextricably linked relationship between emotion, memory, and consumer behavior.
Standardization of the Application Rules for Presumption in Aiding Cybercrime - From the Perspective of 1345 Judgments
Authors: Yiming Yin
Published: December 2023

The crime of aiding cybercriminal activities has shown an explosive growth trend in recent years, evolving to the risk of becoming a pocket crime. To address the existing challenges and issues with this crime, it is crucial to clarify the connotation and denotation of knowingly within the context of this crime and establish a set of relatively standardized and rational inference rules. Through a criminal law analysis of the term knowingly, judicial pathway reviews, and academic theory reflections, the term should be understood as know or clearly know. This interpretation aims to limit judicial scope and alleviate the pressure on the judicial system. The construction of a standardized set of inference rules for knowingly in the context of aiding cybercrime is both necessary and rational. Scholars, both domestic and international, have presented relevant theories, with the current trend favoring an objective-oriented approach complemented by subjective evaluation. After analyzing over a thousand case studies and combining related thoughts, the author proposes a Weighted Scoring Rule and offers several recommendations concerning the current judicial systems institutional design.
Exploring the Training Path of Music Teacher Education on Professionalism
Authors: Ying Li
Published: December 2023

Music education is the unity of the practical activity of music and the practice of education, and has a dual character. In school music education, the practical activities of music have the characteristics of presentational and expressive; the practical activities of education have the characteristics of goal and normative. For a long time, due to the ambiguous understanding of the dual nature of music education practice, it has led to insufficient integration of the music and education disciplines, unclear pathways for music teacher education and training, and little effectiveness in teaching and learning. Therefore, it is necessary to follow the characteristics of their respective disciplines on the basis of finding a point of integration of the disciplines and realizing the integration and unification of the two, so as to improve the mechanism of music teacher education and training.
The Impact of Augmented Reality Technology on Students' Motivation to Learn English
Authors: Li Linjia
Published: December 2023

Research into the application of augmented reality in education remains in its preliminary stages, with a notable dearth of studies exploring the impact and consequences of augmented reality within educational settings. This study aimed to evaluate and gain insight into how an augmented reality mobile application influenced the motivation of junior high school students studying English. Intrinsic motivation theory served as the framework for understanding motivation in a learning context. The study employed the Attention, Relevance, Confidence, and Satisfaction (ARCS) model to elucidate the influence of augmented reality on student motivation. The research instrument utilized was a 34-item questionnaire centered around English learning motivation, based on the Instructional Materials Motivation Survey. A cohort of 200 participants engaged with the augmented reality mobile applications and completed both pre and post-tests. The findings indicated a significant enhancement in students' motivation to learn when utilizing the augmented reality mobile application. Motivational elements, including attention, relevance, confidence, and satisfaction, witnessed marked improvements.
What Comes after 5G? Reflections on the Ideological Attributes of New Communication Technologies
Authors: Fan Bai , Shijian Lin
Published: December 2023

In the 1950s, a vigorous debate on the relationship between technology and societal development emerged in Western theoretical circles, which continues to influence contemporary discussions. Under the banner of progressivism, science and technology were considered neutral, serving as essential drivers of societal progress. The Frankfurt School first proposed the idea that "science and technology are ideologies," exposing the negative aspects of the technological ideology in capitalist societies, while communication political economists also emphasized the "non-neutrality" of technology. The communication environment in Chinese society is currently being rewritten and reshaped by new communication technologies such as 5G, algorithms, and big data. As we anticipate the possibilities brought by these new communication technologies, it is essential to consider their ideological attributes. Technology itself is a result of social processes, and beyond the development logic dominated by the state and capital, more people should be involved in the design and selection of new communication technologies.
An Analytical Study of Sociolinguistic Variations in Urdu Language
Authors: Aftab Saba , Khan Moeen , Aftab Adeena
Published: December 2023

This paper will talk about the sociolinguistic variations in Urdu Language. The study uses the qualitative method it takes account of the interviews conducted by the various senior citizens of Urdu speakers in Pakistan, each interview is based on 4 questions to find out Urdu language variations for this paper. The questions on the value of the Urdu language in todays world, what changes took place in the Urdu language over the period from a lexical and dialectical point of view. The interviews were conducted as semi-structured with the use of different modern and simple tools. The study of this paper will be very helpful for the young generation of Pakistan because all the past studies did not solely talk about sociolinguistics in the Urdu language. It guides them to bring their native or national language with original accents and vocabulary which shows their actual culture and purity of Urdu language.
An Analysis of New Medias Appreciation of Ugliness Anti-Performance and Anti-Authority in Postmodern Contexts
Authors: Zhai Jun
Published: December 2023

In the era of information virtualization, entertainment expression has become the ideological tendency of public discourse. Under the dual drive of technical support and market interests, the appreciation of ugliness has been transformed into individual alienation and emotional behaviour, rebelling against the traditional concept of beauty in cognition and forming a popular and spreading trend of network with grotesqueness, ugliness, and specialisation as symbols. This paper will combine psychological and sociological theories, take the popularity of Nikocado Avocado on YouTube as an example, explore the root causes of the proliferation of ugliness appreciation in todays new media, and further propose ways to purify the social aesthetic environment. Through this study, it was found that the essential reasons are as follows: First, the anti-authority and anti-elite cultural atmosphere formed in the post-modern context makes people more willing to deconstruct beautiful things and oppose traditional mainstream aesthetics; Second, personal jealousy produces subconscious confrontation against beautiful things; third, in order to attract the flow brought by new media and satisfy economic interests, ugliness is performed. To solve this problem, the government needs to play an important role in the media and strengthen the auditing of Internet content. At the same time, relevant departments should disseminate correct aesthetic concepts in society and strengthen the aesthetic education of teenagers.
A Comparative Study of Female Images Between Traditional Drama and Modern DramaTaking Two for the Road and Dou E Yuan as Examples
Authors: Hu Jialian
Published: December 2023

As a mature work of ancient Chinese tragedy, Dou E Yuan not only accuses the cruel reality of disregard for human life, but also has a great influence on later literature and drama. Up to now, it has also appeared as a classic in stage performances, teaching books, etc. Many dramas are deeply inspired by its artistry, theme and realism. The "red literary works" with the same goal as "two for the road" also need a strong political propaganda means to publicize revolutionary thoughts by means of this art form. From the perspective of female images in Dou E Yuan and Two for the Road, this paper comprehensively analyzes the embodiment of female personality characteristics, values and artistic techniques in the two dramas, and compares and investigates the characteristics, situations and changes of gender consciousness of female images in the two dramas.
Leadership Enhancement in Cross-Cultural Management - From the Cultural Dimension
Authors: Huang Liangwei
Published: December 2023

From the perspective of cultural dimension, this paper discusses the importance of cross-cultural management for business leaders and how to enhance cross-cultural leadership in the context of globalisation. Firstly, it proposes to cultivate cross-cultural awareness and multicultural wisdom so that business leaders can reduce misunderstanding and cultural conflicts and enhance team cohesion. Secondly, establishing effective cross-cultural communication is proposed to promote co-operation, enhance influence and create a positive work environment. In addition, adopting appropriate cross-cultural employee incentives is pointed out to meet the needs of employees from different cultural backgrounds and to increase team enthusiasm and innovation. Finally, the importance of continuous learning of cross-cultural leadership thinking and approaches to continuously optimise leadership effectiveness is emphasised. These strategies help business leaders to better adapt to multicultural environments, cope with the challenges posed by cultural differences, and achieve improved teamwork and performance, thus achieving sustained success in the context of globalisation.
The Development of Postmodern Identity in the Characterisation of Do Androids Dream of Electric Sheep
Authors: Li Yang
Published: December 2023

This paper shows that postmodern identity is closely related to the image of cyborg in science fiction. Analyzing the environment and characters in Do Androids Dream of Electric Sheep?, written by Philip K.Dick, offers a deep understanding of characteristics of postmodern identity, which is unstable, fragmented and changeable. The bleak urban landscape where only few people live presents a post war and high tech world that shares features of postmodernism. Besides, the character John Isidore, who is a special, and many escaped androids are all considered as others that should be excluded from the normal society. Hence the dilemma of postmodern identity can be uncovered. To be specific, the android character Rachael Rosen illustrates the fragmentation and reconstruction of postmodern female identity because she is a humanoid robot designed as a woman. Also the combination between a cyborg and a woman gives an insight into the similarities between its identity and her identity. Based on the cyborg theory by Donna Haraway, the criticism by Anne Balsamo in Reading Cyborgs Writing Feminism and the postmodern identity theory by Elena Abrudan, the extent to which Do Androids Dream of Electric Sheep? explored the identity can be disclosed.
Conversation in the News: Comparing News Coverage of Fukushima Water Dispute in AP and CGTN
Authors: Li Xinchen
Published: December 2023

This research paper examines and compares the coverage of the Fukushima water dispute by the Associated Press (AP) and China Global Television Network (CGTN). The study utilizes framing theory analysis to identify differences arising from biases and priorities in news reporting. The paper analyzes the framing used by both outlets and discusses variations in perspectives on Japan's plan and responsibility. It examines the attributions of different narratives and their impact on the audience in each country. Possible causes for these differences are explored, including geopolitical factors, political polarization, and cultural influences. The findings highlight the significant role of media in shaping public opinion and the importance of understanding the diverse influences that contribute to news coverage. The paper concludes that recognizing these influences and seeking diverse viewpoints is crucial for developing a comprehensive understanding of complex issues. However, it acknowledges the limitations of the analysis and highlights the need to consider editorial biases and commercial interests in news reporting.
Media's Impact on the Strengthening of Hostility Between Chinese and American Public
Authors: Guo Zihan
Published: December 2023

This paper shows how the media accomplishes its goals through the language and structure of the article about the Spy Balloon Incident. The analytical process is discussed from agenda-setting theory, Framing theory, and Priming theory, showing the underlying logic of the media and how they increase the hatred of the people of two countries towards each other's country through the spy balloon incident. With this thesis, the paper hope to make readers realize that the media is not necessarily presenting the real facts, but instead may be leading them to think what the polity or consortium behind the media wants them to think. This paper is different from the previous ones because it takes examples from the mainstream media outlets in two separate countries, rather than just looking at media outlets in a single country alone since they might work for the same polity and consortium, which means they have the same interest, and truth will not be revealed to people. This gives a more comprehensive perspective and logical integrity since it considers the issue from opposing sides. Unsurprisingly, the media are leading the crowd by adjusting the language and structure of their paragraphs.
Mindfulness and Mindlessness in Love
Authors: Cao Nicole Zitong
Published: December 2023

The study of love is extensive and affects many areas within relationships between individuals. The following paper examines the concepts of mindfulness and mindlessness, as defined by Langerian mindfulness, within the context of love. The objective of this report is to define the differences and similarities between loving mindfully versus mindlessly and evaluate the impact of both types of love within relationships. The process of engaging in mindful love and enhancing the quality of relationships will be explored, as well as how engaging in mindless love results in emotional disengagement, dissatisfaction, and even conflict. The contrast between the healthy effects of mindful love and the detrimental influences of mindless love is ultimately highlighted. Although being mindlessly in love provides the benefit of ignorance is bliss, mindful love engages all parties in the relationship, constantly bettering each other for a greater change. Essentially, this research paper emphasizes the significance of mindfulness and mindlessness and highlights their coexistence- not only in love- but also in the aspects of life.
Woodblock Printing and Cultural Dissemination
Authors: Zhang Chunhao
Published: December 2023

Printing has played an unparalleled key role in the transmission of culture and the advancement of human civilization. The woodblock printing technology of China during the Sui and Tang periods ignited an innovation in book replication and dissemination, facilitating an enhancement in cultural production capacity and the transmission of culture. During the Song dynasty, advancements in printing technology, whether directly or indirectly, influenced global culture dissemination and the progress of civilization during its overseas transmission, marking a brilliant period in the history of ancient Chinese book publishing and distribution.
Reflections on Inspiring Campus Space Vitality - A Case Study of the Northern Building of the School of Architecture, Including the Surrounding Area of the School Hospital
Authors: Yang Tongyu , Bu Xueyang
Published: December 2023

This paper, through an investigation of the area surrounding the Northern Building of the School of Architecture and the interior spaces of the Northern Building at Tianjin University, summarizes its role in serving as a teaching facility for the college and its positioning in relation to the functions of the hospital. Additionally, the paper, drawing upon analyses and references from similar cases both in China and abroad, primarily explores the Northern Building's potential as a paradigm of open public spaces within the university. It examines the diversification of functions in the area around the school hospital and the Northern Building, interactions with people, feedback from users, and the future possibilities for utilization. The research is conducted through on-site visits and questionnaire surveys to explore the spirit and design concepts conferred by land planning, thereby inspiring vitality in this area and better meeting the needs of users. [1] Moreover, this paper, drawing on theories from environmental psychology and behavioral science, takes a user-centric perspective to explore the spatial aspects of the research area.
Study on the Economic and Social Impacts of Using Mega-events for Urban Regeneration
Authors: Zhang Haoyue
Published: December 2023

The article focuses on the significant economic and social impacts that large-scale events such as the Olympic Games have on the host city. They involve large financial investments, affect millions of people and can strategically transform cities. While these events often improve physical infrastructure, they can also exacerbate inequality and displacement. Disadvantaged communities are often forced to relocate to make way for urban redevelopment projects associated with large-scale activities. Employment and income inequalities may also emerge, with employment opportunities benefiting mainly the middle and upper classes. Legacies such as infrastructure improvements are often unevenly distributed, further marginalising low-income groups. They may also lead to suburbanisation and higher costs of living, resulting in the displacement of residents and the exclusion of disadvantaged groups. In addition, mega-events can place a huge financial burden on taxpayers and are prone to corruption. In short, the neoliberal framework behind mega-events can contribute to the economic, social and geographical polarisation of urban areas.
Ecosystem and its conservation
Authors: Zheng Yuanyin
Published: December 2023

Globally, ecosystems and their restoration and conservation are attracting enormous attention. In recent times, people have gradually realized the importance of ecosystems and the need for ecosystem conservation. At the same time, many scientific conservation measures and methods have been developed, some of which are costly but at least have made a difference in ecosystem restoration. By reviewing a series of related literature, it is possible to see the different views and responses to ecosystems and their conservation in many periods of time, and to realize the seriousness of the situation and challenges facing humanity. The literature review discusses the scientific approach and thinking about the reversibility of ecosystem restoration and rehabilitation.
The role of glial cells in schizophrenic brain
Authors: Deng Yingze
Published: December 2023

Schizophrenia, as a complex neurological disorder, has many distressing symptoms such as hallucinations and some of the common mood or cognitive disorders commonly associated with mental illness. Once suffering from schizophrenia, peoples work and life will be greatly troubled. It is also often associated with other psychiatric disorders, which makes it extremely difficult to treat clinically. However, a diverse of different studies have been able to identify a clear link with neurotransmitter signaling pathways and some brain activity, such as dopamine projection pathways and a significant reduction in gray matter in patients. This research will focus on another dimension, namely the reduction of gray matter volume, to investigate the relationship between oligodendrocyte and astrocyte function and schizophrenia, in an attempt to provide a new vision for the clinical treatment system. In addition, it is expected that this research can also provide a new idea for the development of new drugs for schizophrenia.
Impact of global warming on extreme hot and cold weather
Authors: Dai Zhongrong
Published: December 2023

After the industrial revolution, carbon dioxide emissions have continued to grow rapidly and global warming has become evident. Along with the warming, heat waves, cold waves, and protracted droughts are only a few examples of the extreme weather and climatic phenomena that additionally happened regularly, causing many casualties and property damages. In order to offer a foundation for future decision-making about the avoidance and reaction to severe hot and low temperature weather, we analyzed the reaction of future temperature to rising temperatures in the context of global heating in this study. This paper first analyzed the impact of global warming on extreme hot weather based on the Fifth Assessment Report of the United Nations Intergovernmental Panel on Climate Change(IPCC), in terms of the probability of occurrence of extreme hot weather when the average and temperature variance increase individually and together. Secondly, by examining the causes of the intensified polar vortex brought on by stratospheric warming as in setting of climate warming, the effects of global warming on extreme low degree weather were examined. The findings demonstrated that climate warming had an important impact on the occurrence of extreme high and low temperature weather. Warming on the one hand may make the average temperature and temperature variance change in some regions larger, and extreme high heat occurrences are more likely to occur as the temperature's average and variation both rise. Warming on the other hand will warm the stratosphere, which in turn will cause the asymmetric polar vortex to strengthen, making extreme low temperature events more likely to occur.
Brain computer interface and its application in games for people with physical disability
Authors: Li Yueyang
Published: December 2023

This paper will investigate the fundamentals of the Brain-Computer Interface, including various kinds of implantable Neural Probes, materials used to construct neural probes, and essential methods to detect crucial electrical and chemical signals from brain structures to building well-behaved Brain-Computer Interfaces. Beyond that, this paper will delve deeper into the Brain-Computer Interface (BCI) and its application in the modern time game industry and look into the future perspectives of BCI for helping underrepresented groups that could not utilize traditional input devices.
Common causes and anatomical principles of knee injury and the treatment of different injuries
Authors: Yu Peiyan
Published: December 2023

A knee injury is a common problem that affects people of all ages and activity levels. This review article outlines the anatomy of some common knee injuries and the effective treatment and rehabilitation for different situations. By studying the causes of a knee injury, such as common ones such as pre-cross ligament cracks, semi-monthly plate wear, and pancreatitis, we can determine that treatment for different injuries depends on the specific injury and may include conservative management with rest, physical therapy, surgery, or surgical intervention. At the same time, rehabilitation is an important part of knee injury management, which can have a bearing on when an athlete can return to play and how rehabilitation can improve sports performance for athletic patients.
The effects of lymphocytopenia over the next three to eight months in patients who experience COVID-19 persistent symptoms
Authors: Li Yifei
Published: December 2023

Severe Acute Respiratory Syndrome Coronavirus 2 is a new coronavirus that causes the illness COVID-19. Researchers have established via relevant data that multi-organ destruction, cytokine storm, and lymphocytopenia induced by SARS-CoV-2 are the primary causes of severe illness and patient mortality in COVID-19 patients. And now, as we pass into a new phase, regulatory changes in many nations will cause there to be a shift in the focus of additional research input from the clinical period to the numerous challenges encountered by patients following recovery. We must cope with the growing number of COVID-19 patients who, even after the infection has healed, continue to face functional impairment in their day-to-day life as well as a wide range of long-term symptoms. Their sickness may seriously affect their capacity to carry out the activities of daily living, which will have a detrimental influence on their quality of lives. In addition, lymphopenia has been found to be equally present in these patients recovering from COVID-19 who have undergone the effects of Long-Covid. This study aims to evaluate if the existence of lymphopenia in COVID-19-recovering patients impacts the durability of COVID-19 symptoms by examining individuals with lymphopenia and post-COVID-19 conditions. The purpose of the study is to assess if individuals with lymphocytopenia are more likely than those without lymphocytopenia to be exposed to long-COVID symptoms after eight months.
CRISPR/Cas base-editing systems and their potential applications and prospects
Authors: Gao Tianle , Li Yaojun , Zhao Yiwen
Published: December 2023

CRISPR-Cas9 and its derivatives such as cytosine base editor, adenine base editor and prime editing are an important topic of research today. In recent years, with continuous development and updates by researchers, editing systems have been able to achieve various modifications of target genes. For example, base substitution, insertion and deletion of short fragment genes, etc. have wide applications in plants and animals, etc. However, these editing systems derived from the CRISPR/Cas9 system still face challenges in terms of editing efficiency and accuracy. This review describes the current status of research using editing systems in plant breeding, gene disease treatment, and knockdown of specific genes by establishing animal models, analyzes the advantages and disadvantages of editing technologies, and looks forward to their development.
The pathology, diagnosis and treatment of Alzheimers disease
Authors: Zheng Jiaqi
Published: December 2023

Alzheimers disease (AD) is the main cause of dementia worldwide. Although the pathology of AD is not yet completely understood, it has been widely acknowledged that the biomarkers including beta-amyloid plaques, neurofibrillary tangles and the neuroinflammation induced by these hallmarks contributed to the ADs pathology. Different from the conventional diagnosis of AD which is based on clinical criteria, the modern methods relay on biomarkers imaging and fluid test. Currently, although the treatment of AD is targeted to symptomatic therapy, advanced therapies that target the hallmarks of AD such as anti-beta-amyloid and APOE related therapies are under developing. Here, we will first review the major hypothesis of ADs pathological mechanism and then discuss the methods of diagnosis and treatment of AD which is developed in progress.
Progress in mRNA cancer vaccine research, current limitations, application and future
Authors: Zeng Xinghan
Published: December 2023

Cancer has always been an important topic in the medical field, and human beings have been fighting against cancer for hundreds of years. The methods of cancer treatment have also made great progress and innovation. At present, in addition to the traditional cancer treatment, targeted drug delivery treatment, precision medicine, immunotherapy, etc., are also emerging continuously. Especially in recent years, immunotherapy have received special attention. As an important method of immunotherapy, cancer vaccine has a great prospect, especially mRNA vaccine which belongs to class of nucleic acid vaccine. This review discusses the mechanism of mRNA cancer vaccines playing a role in cancer therapy, and their significant advantages over other types of vaccines. In addition, this review summarizes the current limitations of mRNA vaccines and solutions to these problems. Researchers can also learn about the current application status of mRNA vaccines through this review. Overall, this paper can help readers to have a comprehensive understanding of all aspects of mRNA vaccine, which is of great value to readers who want to conduct further research on mRNA vaccine.
Application of telemedicine in cardiovascular disease prevention and management
Authors: Ma Yiming
Published: December 2023

With the rapid advances of technology and wireless program, the clinical application of telemedicine is received increasingly great attention for its convivence and real-time monitoring. The present study focuses on telemedicine, which is a new tool employed the newly technology like 5G and artificial intelligence to achieve contactless but real-time monitoring patients or subjects for disease management or prevention of future medical events. Telemedicine is useful for help people that in the remote area and do not have access to good medical treatment, more professional experts, better medical examination system or sound surveillance system. It can improve equality and compensate for regional differences. Herein, we summarized trials that have been done recently, and each was compared telemedicine to traditional approaches. Lastly, depending on these reviewed trials, the present study has summarized the advantages and the disadvantages of the telemedicine. We also reviewed the future direction of development of the telemedicine and the facing challenges of telemedicine.
The future of transposon-based CAR-T cell production in immunotherapy
Authors: Lee Szeyui
Published: December 2023

Chimeric Antigen Receptor (CAR) T-cell therapy, being one of the most critical treatment regimens for certain types of lymphomas, leukemias, and multiple myeloma, is experiencing ever-increasing demand. Its high expense and low availability have inflicted many issues among patients in need of this treatment. This paper aims to address these socioeconomic problems by concentrating on the future and current development of transposon-based CAR-T cell production in immunotherapy. Switching viral vectors to transposon-based vectors will greatly decrease the cost and manufacturing time of this personalized product. Transposon vectors also have advantages over viral vectors in sense of DNA carrying capacity and safety matters. Research organizations such as the CARAMBA Project are undertaking new transposon technology in the hope to increase the accessibility of CAR-T cell immunotherapies. Transposon-based CAR-T cell production may pave way for therapeutically and financially satisfying immunotherapies.
A research on the pathological mechanisms of aortic dissection
Authors: Wu Jinhong
Published: December 2023

In this paper, the main content is to investigate which experimental methods are needed in the study of the case mechanism of aortic dissection and how to analyze the results by analyzing the experimental methods. The researchers used RNA sequencing, ChIP-Seq technology, transcription factor prediction, the dual-luciferase reporter assay, immunohistochemical staining, immunofluorescent staining, matrix metalloproteinases (MMP) activity staining, western blot and statistical analysis. The researchers used quantitative RT-PCR, In-Gel zymography, oxidant measurement by dihydroethidium, and flow cytometric analysis of inflammatory cells.
CAR-T therapy for breast cancer
Authors: Liu Botao , Ma Lingyun , Xu Jing , Yan Gege
Published: December 2023

The most common form of cancer among women is breast cancer. With the increasing negative impact of breast cancer on human life and health, scientists have devoted more detailed research on breast cancer treatment and there has been the discovery of a novel CAR-T cell immunotherapy method. Based on the results of the research on CAR-T therapies for breast cancer, this paper summarizes the targets of the therapy for breast cancer, discusses the challenges faced by the treatment of the cancer, and proposes effective strategies to solve the difficulties and overcome the challenges. So far, studies found that breast cancer cells have multiple target sites that they can be combined with. Moreover, such difficulties as weak efficacy and lack of target antigen specificity could be addressed by strategies like dual targeting CAR-T cells and synthetic receptors that combine targeted antigens. These studies provide an effective basis for better understanding the key points and specifics of CAR-T immunotherapy for breast cancer.
The role of LINE-1 transposon plays in cancer: Development, treatment
Authors: Gao Yulun
Published: December 2023

LINE-1 accounts for about more than 15% of the genetic composition in human body, and LINE-1 is the single type of transposon which has the ability of self-initiated transposition. LINE-1 can be inserted into new gene sites through reverse transcription and transposition, leading to genomic instability. Therefore, the body has strict restrictions on not only replication but also transposition, and almost no presence of LINE-1 can be found in casual somatic cells. However, LINE-1 has widespread expression in the vast majority of tumor or cancer tissues, and there is also a lot of evidence indicating that the expression and transposition of LINE-1 are closely related to the emergence and growth of tumors. Expression of LINE-1 is different in tumor cells can serve as a marker for early diagnosis of tumors and an important indicator for evaluating the prognosis of tumor treatment. At the same time, LINE-1 has a potential if being a target for tumor treatment is also expected to be evaluated and validated. This article introduces the operational mechanism and clinical application of LINE-1 in clinical practice, with the aim of providing some reference for the diagnosis and treatment of tumors in clinical practice.
Principle and applications of CRISPR/Cas system
Authors: Zhao Ziqi
Published: December 2023

With the continuous in-depth research and exploration of the CRISPR/Cas9 system by researchers, people's ability to edit biological genes is also constantly improving. In recent years, gene editing technology as a hot technology has been paid more and more attention, it has played an important role in crop breeding, disease treatment, molecular diagnosis and other aspects. Among the gene editing technologies that have been developed, the emerging CRISPR/Cas technology gene editing system has become the most widely used gene editing technology at present. Compared with the first two generations of gene-editing technologies: ZFN and TALEN, CRISPR/Cas system has the advantages of short cycle, high efficiency, low cost, and has great potential for the development of animal and plant breeding. The development of CRISPR/Cas9 technology has promoted the progress of modern life sciences. With these tools for efficient, simple, and precise genome modification, transcriptional regulation, and epigenetic editing, CRISPR systems have the potential to revolutionize genetics, medicine, and agriculture. In this review, the basic principle of CRISPR/Cas technology and its application will be introduced and illustrated.
The impact of global warming on polar marine life
Authors: Li Yiyao
Published: December 2023

The global region is currently facing enormous environmental issues of global warming, which is affecting the entire Earth's ecosystem, especially the natural environment of the oceans. Global warming is a natural phenomenon caused by energy imbalances caused by the cumulative greenhouse effect absorbed and released by the terrestrial air system. The ongoing energy buildup in the terrestrial air system has led to rising temperatures and global warming. Natural disasters and biological chain fracturing are just two examples of the dangers that climate change poses to various facets of humanity. The primary cause of this phenomena is one of the key causes of global farming. In addition, it poses a major threat to the equilibrium of natural ecosystems because of the astounding amount of carbon dioxide emissions produced annually, which directly contributes to an increase in atmospheric carbon dioxide levels. Climate change on the surface of the Earth is a direct outcome of this "greenhouse effect." Environmental pollution, which is getting worse every day, has grown to be a significant global issue and is one of the main causes of global warming. Today, studies on global climate change have amply demonstrated that the Earth's surface temperature has been increasing since the end of the last century. At the same time, this phenomenon has had a significant impact and harm on human survival.
Recent understanding of botulinum toxin and its main detection methods
Authors: Chen Nicole
Published: December 2023

The Botulinum neurotoxin (BoNT) has the title of the most potent poison known to man. It causes a serious condition known as botulism. Guidelines and regulations for the industrial processing of food have made foodborne botulism rarer, but the use of the toxin in pharmaceutical industries and its potential as a bioweapon make detection methods of the toxin a necessary thing. Currently, the Mouse Lethality Bioassay (MLB) is still considered the golden standard of botulinum toxin detection even though it has several limitations. There had subsequently been the development of several alternatives to the MLB, including immunoassays, endopeptidase-based assays, and more. This paper gives an overview of the mechanisms behind botulinum toxins, current methods of detection, and the methods advantages and disadvantages.
The application of NLRP3 inflammasome inhibition in Alzheimers disease therapeutics: Clinical benefits, applications, current limitations, and future development
Authors: Yan Ziyu
Published: December 2023

The severe dementia Alzheimers Disease (AD) has been a neurodegenerative disorder that has troubled many for years due to its difficulty in therapeutic development. Negative regulation of the expression and activation of the NLRP3 inflammasome situated in the hippocampus, entorhinal cortex, blood, and other brain-connected tissues have been reported to contribute to the decrease of pathology and effect of AD. This concise review focuses on the various applications, characteristics, efficacy, and potential of inhibitors of the NLRP3 inflammasome at different stages of the NLRP3 activation and expression pathway in addition to their various limitations that would be places of improvement in future pharmaceutical development. NLRP3 inflammasomes such as IC100 and MCC950 are still in a stage of development, with advantages of high specificity and a large range of function, but with a variety of limiting factors such as the lack of clinical trials and deciding studies, therefore causing a therapy with large potential to be still in a position needing great progress.
The application and development of antibody drug conjugates in cancer therapy
Authors: He Hengkang , Li Enxin , Sheng Sifei
Published: December 2023

Antibody drug conjugates (ADC), as a further modification of monoclonal antibodies, deliver cytotoxic payload to targeted cells. It is a linker connecting a drug with an antibody. As an explosively expanding therapeutic field, ADCs have attracted huge attention among scientists and achieved significant progress during the recent decades. This is because antibody drug conjugates appeared to improve the specificity to target tumors while reducing the toxic side effects. Therefore, the advantages ADCs provided are appealing to both the academic and industrial field. Twelve ADC drugs have recently received FDA approval, while many more remain in the clinical development stage. This review article briefly summarizes the current applications of ADCs in cancer treatment, as well as the existing resistance mechanisms and potential future improvement to them in regard to its components. By providing more information in this field, this article is important in helping people understand antibody drug conjugates.
Effects of dietary structure on obesity and following diseases
Authors: Rui Zechen
Published: December 2023

Due to the quite unhealthy diet that people normally have in recent years, obesity becomes a more and more popular health issue. In recent years, it even becomes a risk factor for deaths. This essay mainly focuses on how the components in diet may cause obesity and other diseases and what components are required to make up of a healthy and balanced diet. To figure out a possible solution for the raising negative consequences brought by obesity, like coronary heart disease and type II diabetes, some investigations have been done. The researchers provided mice with either a diet high in fat or a normal diet to simulate the healthy diet and a quite unhealthy one that humans may take. Some extra tests like glucose tests are taken to show whether the mice have got the symptoms of diabetes. The simulation lasted for two months, an overall higher weight for the mice that were fed with high-fat diet was seen, which indicates that high-fat diet will put more weight on creatures. Also, high-fat diet overall leads to a higher glucose level, which suggests that unhealthy diet may show a decrease in the ability to decompose glucose and therefore causes diabetes. After the data is obtained and the result was drawn, nutritionists may cooperate with doctors and try to figure out the best possible diet for the patients that have very high risk of getting or already suffering from obesity, CHD or type 2 diabetes.
Current status of drug treatment for hand foot mouth disease
Authors: Wu Sirui , Yao Yuxin , You Ziluo
Published: December 2023

Hand, foot, and mouth disease (HFMD) is an infectious disease caused by the intestinal RNA virus. Hand, foot, and mouth disease can occur every season, with no significant difference in specificity. However, the more frequent season of onset is spring and summer, with April to September as the main month of onset. Coastal areas are relatively popular areas of incidence. HFMD is mainly characterized by herpes with different sizes and clinical manifestations, such as in the hands, feet, and mouth. This article categorizes drugs into two categories: antiviral drugs and antiviral prevention. In describing antiviral drugs, they are classified them into traditional Chinese medicine and non-traditional Chinese medicine. At the same time, the two most effective and commonly used drugs or methods are summarized for preventing HFMD, namely the EV-71 virus vaccine for HFMD, and the washable hand sanitizer that prevents HFMD by blocking the transmission path of the virus. A comprehensive description and summary of the current treatment methods and drugs for combating and treating HFMD. This article provides a comprehensive description of different drugs by elaborating on their advantages and therapeutic principles, combined with their related pharmacological effects.
The role of the 14-3-3 protein family in disease
Authors: Guo Lele
Published: December 2023

14-3-3 protein is a dimer protein extracted from bovine brain cells, a highly conserved protein prevalent in eukaryotes. There are seven subtypes in mammals, respectively , , , , , and . The subtypes differ in content and function. It was found that the protein interacts with corresponding ligand protein in regulating life activities, such as: cell cycle regulation, cell development, the transmission of cell signal molecules plays an important role. At the same time, related regulators have been developed based on cell-cell interaction. In recent years, the exploration of 14-3-3 protein has been deepening gradually. Inhibition or stabilizers of the 14-3-3 protein in question have been studied or discovered, and have become a new target for treating diseases. This paper reviews the effects of 14-3-3 protein on neurological disease, cardiovascular and cerebrovascular diseases and cancer are reviewed. It is found that protein subtypes affect the occurrence of diseases.
Binding modes in ligand-docked hepatitis B virus core protein simulated by a Monte Carlo method
Authors: Gao Zixuan
Published: December 2023

Hepatitis B virus core protein (HBV Cp) is closely involved in the viral assembly, nuclear functions, compartment for reverse transcription, and intra-cellular trafficking. Therefore, modulation of HBV Cp assembly is a promising method to control HBV infection in both preclinical and clinical studies. In this paper, two ligands of HAP18 and AT-130, as representations of heteroaryldihydropyrimidines and phenylpropenamides, have been chosen. Their Binding modes and conformations docking with the HBV Cp have been simulated by a Monte Carlo method. Strong polar contact in HAP18-bound and relatively weak interaction dominated by the Lennard-Jones potential in AT130-bound have been observed, respectively. Although different binding modes result in different assembly behaviors of HBV core protein, both of them strongly influence the quaternary structure of the Cp assembly, changing the spatial relationship between dimers, and inducing noninfective Cp misassembly. The simulation is expected to be helpful to get some insight into the antiviral mechanism of HBV Cp assembly modulation.
Albumin-binding prodrugs use in treating cancer
Authors: Yu Zifei
Published: December 2023

Throughout the past few decades, the use of targeted delivery methods for cancer therapies has dramatically increased. Prodrug, a type of drug that can apply the targeting therapy, is defined as chemicals that undergo chemical transformations after enters the patients body. They will be activated and release the parent drug after reached the targeted site. Both passively and actively targeted therapies are explored. The albumin-binding prodrug, a model of prodrug applying passively targeted therapy, this paper introduces the enhanced permeability and retention (EPR) effect to target the tumor site. Besides, since the human serum albumin is an endogenous substance originated from human body, its nanoparticles is able to carry the anticancer drugs to elongate the circulatory half-lives of drugs since it will not be rejected by the immune system. However, the activated compound's systemic toxicity and the dearth of information regarding the biodistribution of prodrugs are two possible disadvantages. Moreover, some exogenous albumin formulations are prohibited from participating in clinical trials because of their very poor delivery efficiency. In conclusion, the albumin-binding prodrug is a cancer treating therapy that has great promise and great capability despite the potential risks.