bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Rapid COVID-19 Antigen Test in the Emergency Department as a Decision-Support Tool
Authors: Meltzer Lilac , Amit Sharon , Gilboa Mayan , Tal Ilana , Mechnik Bella , Irony Avi , Engelrad Hindi , Epstein Avi , Frenkel-Nir Yael , Levy Yuval , Kreiss Yitshak , Regev-Yochay Gili
Published: January 2023

The emergency department (ED) is the initial point of contact between hospital staff and patients potentially infected with SARS-CoV-2, thus, prevention of inadvertent exposure to other patients is a top priority. We aimed to assess whether the introduction of antigen-detecting rapid diagnostic tests (Ag-RDTs) to the ED affected the likelihood of unwanted SARS-CoV-2 exposures. In this retrospective single-center study, we compared the rate of unwarranted exposure of uninfected adult ED patients to SARS-CoV-2 during two separate research periods; one before Ag-RDTs were introduced, and one with Ag-RDT used as a decision-support tool. The introduction of Ag-RDTs to the ED significantly decreased the relative risk of SARS-CoV-2-negative patients being incorrectly assigned to the COVID-19 designated site (“red ED”), by 97%. There was no increase in the risk of SARS-CoV-2-positive patients incorrectly assigned to the COVID-19-free site (“green ED”). In addition, duration of ED admission was reduced in both the red and the green ED. Therefore, implementing the Ag-RDT-based triage protocol proved beneficial in preventing potential COVID-19 nosocomial transmission.
The Mycobacterium bovis BCG GroEL1 Contributes to Isoniazid Tolerance in a Dormant-Like State Model
Authors: Zeng Sheng , Yang Dong , Rens Céline , Fontaine Véronique
Published: January 2023

Due to the Mycobacterium tuberculosis complex, including M. tuberculosis and M. bovis, tuberculosis still causes 1.6 million deaths per year. Therefore, efforts to improve tuberculosis treatment are necessary. We previously showed that the GroEL1 protein is involved in antibiotic intrinsic resistance. Indeed, the M. bovis BCG cpn60.1 gene (encoding GroEL1)-disrupted strain (Δcpn60.1) exhibits higher rifampicin and vancomycin susceptibility due to defective cell wall integrity. Here, we show that during hypoxia-triggered growth stasis, in the Wayne dormancy model, the mutant exhibited comparable rifampicin and ethionamide susceptibility but higher isoniazid susceptibility compared to the wild-type strain. Although the Δcpn60.1 strain showed compromised induction of the DosR regulon, growth stasis was achieved, but an ATP burst and a higher reactive oxygen species (ROS) production were observed in the isoniazid-treated Δcpn60.1 strain. GroEL1 could contribute to INH tolerance by reducing ROS.
Luteibacter flocculans sp. nov., Isolated from a Eutrophic Pond and Isolation and Characterization of Luteibacter Phage vB_LflM-Pluto
Authors: Friedrich Ines , Kuritsyn Alisa , Hertel Robert , Daniel Rolf
Published: January 2023

Luteibacter is a genus of the Rhodanobacteraceae family. The present study describes a novel species within the genus Luteibacter (EIF3T). The strain was analyzed genomically, morphologically and physiologically. Average nucleotide identity analysis revealed that it is a new species of Luteibacter. In silico analysis indicated two putative prophages (one incomplete, one intact). EIF3T cells form an elliptical morphotype with an average length of 2.0 µm and width of 0.7 µm and multiple flagella at one end. The bacterial strain is an aerobic Gram-negative with optimal growth at 30 °C. EIF3T is resistant towards erythromycin, tetracycline and vancomycin. We propose the name Luteibacter flocculans sp. nov. with EIF3T (=DSM 112537T = LMG 32416T) as type strain. Further, we describe the first known Luteibacter-associated bacteriophage called vB_LflM-Pluto.
Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta
Authors: Werner Katharina A. , Feyen Lara , Hübner Tobias , Brüggemann Nicolas , Prost Katharina , Grohmann Elisabeth
Published: January 2023

Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes blaCTX-M, blaIMP, and blaTEM conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in blaCTX-M and blaTEM copy numbers and a mean increase in blaIMP copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta.
Ferric Hydroxide Recovery from Iron-Rich Acid Mine Water with Calcium Carbonate and a Gypsum Scale Inhibitor
Authors: Mogashane Tumelo Monty , Maree Johannes Philippus , Mujuru Munyaradzi , Mphahlele-Makgwane Mamasegare Mabel , Modibane Kwena Desmond
Published: January 2023

The focus of this study was to improve the Reverse Osmosis Cooling (ROC) process by using CaCO3 for neutralization and selective recovery of Fe(OH)3 at pH 3.5. By using a specific inhibitor, ferric hydroxide was recovered separately from gypsum and other metals present in mine water. Ferric hydroxide was processed to pigment, a product that is imported and used as colorant in paints and tiles. In addition to pigment recovery, aluminum hydroxide and calcium carbonate can also be recovered from mine water. The following conclusions were made: (i) the rate of gypsum crystallization, in the absence of Fe3+, is influenced by the over saturation concentration in solution, the seed crystal concentration and temperature; (ii) gypsum crystallization from an over-saturated solution, in the presence of Fe(OH)3 sludge, required an inhibitor dosage of 100 mg/L to keep gypsum in solution for a period of 30 min; (iii) gypsum crystallization from an over-saturated solution, in the presence of both Fe(OH)3 sludge and CaCO3 reactant, required a higher inhibitor dosage than 100 mg/L to keep gypsum in solution for a period of 30 min. A dosage of 200 mg/L kept gypsum in solution for the total reaction period; (iv) when only Fe(OH)3 is present in the slurry, gypsum inhibition is more effective when Fe(OH)3 sludge is allowed to settle after the initial mixing; (v) when both Fe(OH)3 and CaCO3 are present in the slurry, gypsum inhibition is more effective when the inhibitor is added over a period of time (10 min) rather than applying the total dosage at time zero; (vi) Fe(OH)3 can be changed to yellow pigment (Goethite) by heating to 150 °C and to red pigment (Hematite) by heating to 800 °C. Pigment of nano particle size was produced; (vii) in the case of Na2CO3, the TDS increased from 12,660 mg/L in the feed to 13,684 mg/L due to the replacement of metal ions (Fe3+, Al3+, Fe2+, Mn2+ and Ca2+) with Na+ in solution. In the case where CaCO3 was used for the removal of Fe3+ and Al3+, Ca(OH)2 for the removal of Fe2+, Mn2+, and Na2CO3 for the removal of Ca2+, the TDS dropped from 12,661 mg/L to 2288 mg/L, due to gypsum precipitation. The alkali cost in the case of calcium alkalis amounted to ZAR29.43/m3 versus ZAR48.46/m3 in the case of Na2CO3.
The Changes in Red Blood Cell Indices That Occur in Pre-Diabetic Patients of all Ethnicities from the 25–45 Years of Age: A Protocol for a Systematic Review and Meta-Analysis
Authors: Mzimela Nomusa Christina , Sosibo Aubrey Mbulelo , Ngubane Phikelelani Siphosethu , Khathi Andile
Published: January 2023

Introduction: Pre-diabetes is an intermediate, asymptomatic state between normoglycaemia and the onset of type 2 diabetes mellitus (T2D). Recent reports indicate that there are sub-clinical changes observed in red blood cells during pre-diabetes. This systematic review protocol will provide an outline of all procedures in the synthesis of the available data on the changes in red blood cell indices. Methods and Analysis: This protocol was prepared by adhering to the PRISMA 2015 guidelines for reporting protocols. Published clinical studies that involve observation, whether it is cross-sectional, comparative cross-sectional, case-control or cohort study designs that involve normal/non-diabetic and pre-diabetes reports were used. Additionally, this was accomplished by using clinical MeSH headings to search on MEDLINE, COCHRANE library and African Journal Online. Three reviewers (NCM, AMS & AK) screened all the results for eligibility criteria. Then, Downs and Black checklist was used to check the risk of bias. Review Manager v5.4 Forrest plot was used for meta-analysis and sensitivity analysis. Strength of evidence was then assessed using the Grading of Recommendations Assessment, Development, and Evaluation approach (GRADE). Results and Conclusion: This protocol will give direction on the exploration of articles that report on changes in red blood cell indices in the pre-diabetic state. The results obtained from this protocol will further give direction on the research to be done at in the eThekwini district of South Africa. Ethics and Dissemination: The data that will be analyzed will be data that has already been published thus there will be no data collection from subjects. Therefore, no ethical clearance is required. Registration Details: This protocol has been registered with the International Prospective Registry of Systematic Reviews (PROSPERO) registration number “CRD42020189080” dated 05-07-2020.
Optimized Protocol for Isolation and Culture of Murine Neonatal Primary Lung Fibroblasts
Authors: Fuentes-Mateos Rocío , Santos Eugenio , Fernández-Medarde Alberto
Published: January 2023

During all the stages of lung development, the lung mesoderm (or mesenchyme) is closely related to the endoderm, and their cross-regulation promotes, controls, and drives all lung developmental processes. Generation of 3D organoids in vitro, RNA assays, and mitochondrial respiration studies are used to analyze lung development and regeneration to better understand the interactions between epithelium and mesenchyme, as well as for the study of redox alterations and the metabolic status of the cells. Moreover, to avoid using immortalized cell lines in these in vitro approaches, standardized murine neonatal primary lung fibroblast isolation techniques are essential. Here, we present an optimized method to isolate, culture, and freeze primary lung fibroblasts from neonatal lungs. Our current method includes step-by-step instructions accompanied by graphical explanations and critical steps.
Recent Advances in Black TiO2 Nanomaterials for Solar Energy Conversion
Authors: Liao Lijun , Wang Mingtao , Li Zhenzi , Wang Xuepeng , Zhou Wei
Published: January 2023

Titanium dioxide (TiO2) nanomaterials have been widely used in photocatalytic energy conversion and environmental remediation due to their advantages of low cost, chemical stability, and relatively high photo-activity. However, applications of TiO2 have been restricted in the ultraviolet range because of the wide band gap. Broadening the light absorption of TiO2 nanomaterials is an efficient way to improve the photocatalytic activity. Thus, black TiO2 with extended light response range in the visible light and even near infrared light has been extensively exploited as efficient photocatalysts in the last decade. This review represents an attempt to conclude the recent developments in black TiO2 nanomaterials synthesized by modified treatment, which presented different structure, morphological features, reduced band gap, and enhanced solar energy harvesting efficiency. Special emphasis has been given to the newly developed synthetic methods, porous black TiO2, and the approaches for further improving the photocatalytic activity of black TiO2. Various black TiO2, doped black TiO2, metal-loaded black TiO2 and black TiO2 heterojunction photocatalysts, and their photocatalytic applications and mechanisms in the field of energy and environment are summarized in this review, to provide useful insights and new ideas in the related field.
Low Loss Vertical TiO2/Polymer Hybrid Nano-Waveguides
Authors: Doughan Isaac , Oyemakinwa Kehinde , Ovaskainen Olli , Roussey Matthieu
Published: January 2023

This article proposes a novel demonstration of a low-loss polymer channel hybridized with a titania core leading to a nano-waveguide elongated in the normal direction to the substrate. It is aimed at using the quasi-transverse magnetic (TM) mode as the predominant mode in compact photonic circuitry. A detailed design analysis shows how a thin layer of a higher-refractive index material in a trench within the core of the waveguide can increase the confinement and reduce the propagation losses. This thin layer, produced by atomic layer deposition, covers the entire polymer structure in a conformal manner, ensuring both a reduction of the surface roughness and a stronger field confinement. The trench can be made at any place within the polymer channel and therefore its position can be tuned to obtain asymmetric modal distribution. The waveguide is demonstrated at telecom wavelengths, although the material’s properties enable operation over a large part of the electromagnetic spectrum. We measured propagation losses as low as 1.75 ± 0.32 dB/cm in a 200 nm × 900 nm section of the waveguide core. All processes being mass-production compatible, this study opens a path towards easier integrated-component manufacture.
The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design
Authors: Ruijter Nienke , Soeteman-Hernández Lya G. , Carrière Marie , Boyles Matthew , McLean Polly , Catalán Julia , Katsumiti Alberto , Cabellos Joan , Delpivo Camilla , Sánchez Jiménez Araceli , Candalija Ana , Rodríguez-Llopis Isabel , Vázquez-Campos Socorro , Cassee Flemming R. , Braakhuis Hedwig
Published: January 2023

The Safe-by-Design (SbD) concept aims to facilitate the development of safer materials/products, safer production, and safer use and end-of-life by performing timely SbD interventions to reduce hazard, exposure, or both. Early hazard screening is a crucial first step in this process. In this review, for the first time, commonly used in vitro assays are evaluated for their suitability for SbD hazard testing of nanomaterials (NMs). The goal of SbD hazard testing is identifying hazard warnings in the early stages of innovation. For this purpose, assays should be simple, cost-effective, predictive, robust, and compatible. For several toxicological endpoints, there are indications that commonly used in vitro assays are able to predict hazard warnings. In addition to the evaluation of assays, this review provides insights into the effects of the choice of cell type, exposure and dispersion protocol, and the (in)accurate determination of dose delivered to cells on predictivity. Furthermore, compatibility of assays with challenging advanced materials and NMs released from nano-enabled products (NEPs) during the lifecycle is assessed, as these aspects are crucial for SbD hazard testing. To conclude, hazard screening of NMs is complex and joint efforts between innovators, scientists, and regulators are needed to further improve SbD hazard testing.
Synthesis, Characterization, and Electrochemical Evaluation of Copper Sulfide Nanoparticles and Their Application for Non-Enzymatic Glucose Detection in Blood Samples
Authors: Tetyana Phumlani , Mphuthi Ntsoaki , Jijana Abongile Nwabisa , Moloto Nosipho , Shumbula Poslet Morgan , Skepu Amanda , Vilakazi Lea Sibulelo , Sikhwivhilu Lucky
Published: January 2023

Glutathione-capped copper sulfide (CuxSy) nanoparticles with two different average sizes were successfully achieved by using a simple reduction process that involves only changing the reaction temperature. Temperature-induced changes in the size of CuxSy nanoparticles resulted in particles with different optical, morphological, and electrochemical properties. The dependence of electrochemical sensing properties on the sizes of CuxSy nanoparticles was studied by using voltammetric and amperometric techniques. The spherical CuxSy nanoparticles with the average particle size of 25 ± 0.6 nm were found to be highly conductive as compared to CuxSy nanoparticles with the average particle size of 4.5 ± 0.2 nm. The spherical CuxSy nanoparticles exhibited a low bandgap energy (Eg) of 1.87 eV, resulting in superior electrochemical properties and improved electron transfer during glucose detection. The sensor showed a very good electrocatalytic activity toward glucose molecules in the presence of interference species such as uric acid (UA), ascorbic acid (AA), fructose, sodium chloride, and sucrose. These species are often present in low concentrations in the blood. The sensor demonstrated an excellent dynamic linear range between 0.2 to 16 mM, detection limit of 0.2 mM, and sensitivity of 0.013 mA/mM. The applicability of the developed sensor for real field determination of glucose was demonstrated by use of spiked blood samples, which confirmed that the developed sensor had great potential for real analysis of blood glucose levels.
Cover Crop Termination Method and N Fertilization Effects on Sweet Corn Yield, Quality, N Uptake, and Weed Pressure
Authors: Sunuwar Sachina , Siller Arthur , Glaze-Corcoran Samantha , Hashemi Masoud
Published: January 2023

Cover cropping is vital for soil health. Timing and method of termination are major factors influencing the agroecological benefits of cover crops. Delay in the termination of cover crops results in greater biomass production. Likewise, incorporation of cover crops during termination often speeds residue mineralization compared to no-till systems. We used four termination strategies for a late-terminated winter rye–legume mix (in tilled and no-till systems) and four N application rates in the succeeding sweet corn crop to examine how cover crop termination affected N response in sweet corn as well as the independent effects of N application rate and cover crop termination method. The experiment was conducted using a randomized complete block design with four replications. Increasing N fertilization up to 144 kg N ha−1 promoted yield and quality in sweet corn as well as summer weed growth. The cover crop termination method did not affect sweet corn response to N fertilizer. This suggests that when rye is terminated late in the spring before planting cash crops, the incorporation of its residues may not greatly affect the soil N dynamics. This indicates that decisions to incorporate rye residues may be taken by farmers with an eye mainly towards management issues such as weed control, environmental impacts, and soil health.
Molecular Characterizations of the Coagulase-Negative Staphylococci Species Causing Urinary Tract Infection in Tanzania: A Laboratory-Based Cross-Sectional Study
Authors: Phillip Shukrani , Mushi Martha F. , Decano Arun Gonzales , Seni Jeremiah , Mmbaga Blandina T. , Kumburu Happiness , Konje Eveline T. , Mwanga Joseph R. , Kidenya Benson R. , Msemwa Betrand , Gillespie Stephen , Maldonado-Barragan Antonio , Sandeman Alison , Sabiti Wilber , Holden Mathew T. G. , Mshana Stephen E.
Published: January 2023

Background: There is a growing body of evidence on the potential involvement of coagulase-negative Staphylococci (CoNS) in causing urinary tract infections (UTIs). The aim of this study was to delineate virulence potential, antimicrobial resistance genes, and sequence types of CoNS isolated from patients with UTI symptoms and pyuria in Tanzania. Methods: CoNS from patients with UTI symptoms and more than 125 leucocytes/μL were retrieved, subcultured, and whole-genome sequenced. Results: Out of 65 CoNS isolates, 8 species of CoNS were identified; Staphylococcus haemolyticus, n = 27 (41.5%), and Staphylococcus epidermidis, n = 24 (36.9%), were predominant. The majority of S. haemolyticus were sequence type (ST) 30, with 8 new ST138-145 reported, while the majority of S. epidermidis were typed as ST490 with 7 new ST1184-1190 reported. Sixty isolates (92.3%) had either one or multiple antimicrobial resistance genes. The most frequently detected resistance genes were 53 (21%) dfrG, 32 (12.9%) blaZ, and 26 (10.5%) mecA genes conferring resistance to trimethoprim, penicillin, and methicillin, respectively. Out of 65 isolates, 59 (90.8%) had virulence genes associated with UTI, with a predominance of the icaC 47 (46.5%) and icaA 14 (13.9%) genes. Conclusion:S. haemolyticus and S. epidermidis harboring icaC, dfrG, blaZ, and mecA genes were the predominant CoNS causing UTI in Tanzania. Laboratories should carefully interpret the significant bacteriuria due to CoNS in relation to UTI symptoms and pyuria before labeling them as contaminants. Follow-up studies to document the outcome of the treated patients is needed to add more evidence that CoNS are UTI pathogens.
Current Limitations of Staph Infection Diagnostics, and the Role for VOCs in Achieving Culture-Independent Detection
Authors: Jenkins Carrie L. , Bean Heather D.
Published: January 2023

Staphylococci are broadly adaptable and their ability to grow in unique environments has been widely established, but the most common and clinically relevant staphylococcal niche is the skin and mucous membranes of mammals and birds. S. aureus causes severe infections in mammalian tissues and organs, with high morbidities, mortalities, and treatment costs. S. epidermidis is an important human commensal but is also capable of deadly infections. Gold-standard diagnostic methods for staph infections currently rely upon retrieval and characterization of the infectious agent through various culture-based methods. Yet, obtaining a viable bacterial sample for in vitro identification of infection etiology remains a significant barrier in clinical diagnostics. The development of volatile organic compound (VOC) profiles for the detection and identification of pathogens is an area of intensive research, with significant efforts toward establishing breath tests for infections. This review describes the limitations of existing infection diagnostics, reviews the principles and advantages of VOC-based diagnostics, summarizes the analytical tools for VOC discovery and clinical detection, and highlights examples of how VOC biomarkers have been applied to diagnosing human and animal staph infections.
Recent Advances in Optical Hydrogen Sensor including Use of Metal and Metal Alloys: A Review
Authors: Pathak Akhilesh Kumar , Verma Sneha , Sakda Natsima , Viphavakit Charusluk , Chitaree Ratchapak , Rahman B. M. Azizur
Published: January 2023

Optical sensing technologies for hydrogen monitoring are of increasing importance in connection with the development and expanded use of hydrogen and for transition to the hydrogen economy. The past decades have witnessed a rapid development of optical sensors for hydrogen monitoring due to their excellent features of being immune to electromagnetic interference, highly sensitive, and widely applicable to a broad range of applications including gas sensing at the sub-ppm range. However, the selection of hydrogen selective metal and metal alloy plays an important role. Considering the major advancements in the field of optical sensing technologies, this review aims to provide an overview of the recent progress in hydrogen monitoring. Additionally, this review highlights the sensing principles, advantages, limitations, and future development.
The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro
Authors: El Samarji Mona , Younes Maria , El Khoury Marianne , Haykal Tony , Elias Nazira , Gasilova Natalia , Menin Laure , Houri Ahmad , Machaka-Houri Nisrine , Rizk Sandra
Published: January 2023

Background: Sternbergia clusiana belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of Sternbergia clusiana (ScBEE) on breast cancer cells in vitro and to further reveal the underlying cellular mechanism. Methods: An MTS cell viability assay was performed on MDA-MB-231 and MCF-7 cells, along with cell cycle analysis, cell death ELISA, Western blot analysis and an ROS production assay to decipher the mechanism of death. LC-MS/MS was also performed to identify the chemical composition of this ethanolic extract. Results: The results show a selective antiproliferative effect on both cell lines with no effect on normal mesenchymal stem cells. Further analysis suggested the activation of the apoptotic pathway as reflected by the increase in cellular and DNA fragmentation and alterations in apoptotic proteins such as Bax, Bcl-2 and c-PARP. ScBEE was also found to exhibit antioxidant effect, as shown by a decrease in ROS production. The underlying mechanism of action was explained by the presence of several bioactive compounds identified by LC-MS/MS, including alkaloids, terpenoids and phenols, which are elaborated in the manuscript. Conclusion: This study highlights the antioxidant and anticancerous properties of S.clusiana for breast cancer treatment.
Metabolomics and Transcriptomics Provide Insights into Lipid Biosynthesis in the Embryos of Walnut (Juglans regia L.)
Authors: Liang Manman , Zhang Xuemei , Dong Qinglong , Li Han , Guo Suping , Luan Haoan , Jia Peng , Yang Minsheng , Qi Guohui
Published: January 2023

Walnut (Juglans regia L.) is an important woody oilseed tree species due to its commercial value. However, the regulation mechanism of walnut oil accumulation is still poorly understood, which restricted the breeding and genetic improvement of high-quality oil-bearing walnuts. In order to explore the metabolic mechanism that regulates the synthesis of walnut oil, we used transcriptome sequencing technology and metabolome technology to comprehensively analyze the key genes and metabolites involved in oil synthesis of the walnut embryo at 60, 90, and 120 days after pollination (DAP). The results showed that the oil and protein contents increased gradually during fruit development, comprising 69.61% and 18.32% of the fruit, respectively, during ripening. Conversely, the contents of soluble sugar and starch decreased gradually during fruit development, comprising 2.14% and 0.84%, respectively, during ripening. Transcriptome sequencing generated 40,631 unigenes across 9 cDNA libraries. We identified 51 and 25 candidate unigenes related to the biosynthesis of fatty acid and the biosynthesis of triacylglycerol (TAG), respectively. The expression levels of the genes encoding Acetyl-CoA carboxylase (ACCase), long-chain acyl-CoA synthetases (LACS), 3-oxoacyl-ACP synthase II (KASII), and glycerol-3-phosphate acyl transfer (GPAT) were upregulated at 60 DAP relative to the levels at 90 and 120 DAP, while the stearoyl-ACP-desaturase (SAD) and fatty acid desaturase 2 (FAD2) genes were highly abundantly expressed during all walnut developmental periods. We found that ABSCISIC ACID INSENSEITIVE3 (ABI3), WRINKLEDl (WRI1), LEAFY COTYLEDON1 (LEC1), and FUSCA3 (FUS3) may be key transcription factors involved in lipid synthesis. Additionally, the metabolomics analysis detected 706 metabolites derived from 18 samples, among which, 4 are implicated in the TAG synthesis, 2 in the glycolysis pathway, and 5 in the tricarboxylic acid cycle (TCA cycle) pathway. The combined analysis of the related genes and metabolites in TAG synthesis showed that phospholipid:diacylglycerol acyltransferase (PDAT) genes were highly abundantly expressed across walnut fruit developmental periods, and their downstream metabolite TAG gradually accumulated with the progression of fruit development. The FAD2 gene showed consistently higher expression during fruit development, and its downstream metabolites 18:2-PC and 18:3-PC gradually accumulated. The ACCase, LACS, SAD, FAD2, and PDAT genes may be crucial genes required for walnut oil synthesis. Our data will enrich public databases and provide new insights into functional genes related to lipid metabolism in walnut.
Dataset of 3β-Corner and 3β-Corner-like Structures
Authors: Rudnev Vladimir R. , Kulikova Liudmila I. , Nikolsky Kirill S. , Petrovsky Denis V. , Stepanov Alexander A. , Malsagova Kristina A. , Kaysheva Anna L.
Published: January 2023

The study is devoted to the creation of a dataset of protein structural motifs of the 3β-corner type. The relevance and importance of creating a dataset of 3β-corners is determined by the fact that this structure can be an embryo or a ready-made structural block in the process of protein folding, and can also act as an independent object of research in the field of structural biology. The dataset also contains 3β-corner-like structures that are geometrically similar to 3β-corners. The dataset consists of 45,896 structures. For each motif, its characteristics are presented: the name of the protein in which the 3β-corner is recognized, the method and resolution of the protein structure, the coordinates of localization in the protein, the secondary structure of the amino acid sequence, the gyration radius, the solvent-accessible area, and the composition of the elements of the secondary structure. The dataset will allow a comprehensive study of structures on a large scale and advance the understanding of the features and patterns of their structural organization.
A Review of Real-Time Fault Diagnosis Methods for Industrial Smart Manufacturing
Authors: Yan Wenhao , Wang Jing , Lu Shan , Zhou Meng , Peng Xin
Published: January 2023

In the era of Industry 4.0, highly complex production equipment is becoming increasingly integrated and intelligent, posing new challenges for data-driven process monitoring and fault diagnosis. Technologies such as IIoT, CPS, and AI are seeing increasing use in modern industrial smart manufacturing. Cloud computing and big data storage greatly facilitate the processing and management of industrial information flow, which helps the development of real-time fault diagnosis (RTFD) technology. This paper provides a comprehensive review of the latest RTFD technologies in the field of industrial process monitoring and machine condition monitoring. The RTFD process is introduced in detail, starting with the data acquisition process. The current RTFD methods are divided into methods based on independent feature extraction, methods based on “end-to-end” neural networks, and methods based on qualitative knowledge reasoning from a new perspective. In addition, this paper discusses the challenges and potential trends of RTFD in future development to provide a reference for researchers focusing on this field.
Health-Promoting Potential of Millet: A Review
Authors: Sabuz Ashfak Ahmed , Rana Md Rahmatuzzaman , Ahmed Tanvir , Molla Mohammad Mainuddin , Islam Nazmul , Khan Hafizul Haque , Chowdhury Golam Ferdous , Zhao Qingyu , Shen Qun
Published: January 2023

Being a key source of animal food, millet production has been sharply increasing over the last few years in order to cope with the dietary requirements of the ever-increasing world population. It is a splendid source of essential nutrients such as protein, carbohydrates, fat, minerals, vitamins, and also some other bioactive compounds that eventually help through multiple biological activities, including antioxidant, anti-hyperglycemic, anti-cholesterol, anti-hypertensive, anthropometric effects and regulation of gut microbiota composition. These bioactive compounds, nutrients, and functions of cereal grains can be affected by processing techniques such as decortication, soaking, malting, milling, fermentation, etc. This study discusses the nutritional and functional properties of millet-incorporated foods and their impact on health, based on around 150 articles between 2015 and 2022 from the Web of Science, Google Scholar, Food and Agriculture Organization of the United Nations (FAO), Breeding Bid Survey (BBS), and FoodData Central (USDA) databases. Analyzing literature reviews, it is evident that the incorporation of millet and its constituents into foodstuffs could be useful against undernourishment and several other health diseases. Additionally, this review provides crucial information about the beneficial features of millet, which can serve as a benchmark of guidelines for industry, consumers, researchers, and nutritionists.
Innovative Green Approach for Extraction of Piperine from Black Pepper Based on Response Surface Methodology
Authors: Lwamba Charles , Aboushanab Saied A. , Ambati Ranga Rao , Kovaleva Elena G.
Published: January 2023

Bioactive compounds like piperine (alkaloids) offer a variety of health benefits due to their biological and pharmacological potential. Piperine has been revealed to have anti-inflammatory, anti-aging, anti-diabetes, anti-bacterial, anti-ulcer, and anti-carcinogenic characteristics. Recent research has been conducted to extract piperine using effective and environmentally friendly techniques. In this study, we sought to assess the potential and efficacy of natural deep eutectic solvents to extract piperine from black pepper seeds using an ultrasound-assisted extraction technique. A Box–Behnken design combined with response surface methodology was used to evaluate the optimum extraction conditions of piperine. Extraction efficiency was evaluated based on the extraction yields of piperine, antioxidant activity, total polyphenols, and total flavonoids. The results showed that the choline chloride-citric acid-1,2-propylene glycol combination (1:2:2 molar ratio) with 25% (v/v) of water was the most effective at extracting piperine from black pepper. It was found that the extraction yield of piperine was significantly influenced by the liquid–solid ratio and extraction time. The optimal extraction conditions were determined and it was found that antioxidant activities and total polyphenol content in the piperine-rich extracts were remarkably related to the piperine content. The piperine extract purity was found to be 90%. Our results indicate that black pepper could be used as a functional food application.
Exposure to Volatile Organic Compounds in Paint Production Plants: Levels and Potential Human Health Risks
Authors: Ghobakhloo Safiye , Khoshakhlagh Amir Hossein , Morais Simone , Mazaheri Tehrani Ashraf
Published: January 2023

A wide range of volatile organic solvents, including aliphatic and aromatic hydrocarbons, alcohols, and ketones, are used in the production of paints, and they comprise more than 30% of the ingredients of paints. The present study was designed to evaluate the occupational exposure to 15 volatile organic compounds (VOCs, including benzene, toluene, ethylbenzene, xylene, styrene, n-hexane, n-heptane, n-nonane, trichloroethylene, tetrachloroethylene, n-butyl acetate, n-octane, n-decane, dichlorofluoromethane, and acetone) in Iranian paint production factories and subsequently, the associated health risks. The samples were collected from the respiratory zone of workers using the NIOSH 1501 method, and their qualitative and quantitative characterization was performed using gas chromatography-mass spectrometry and gas chromatography-flame ionization detector, respectively. The individual concentrations of VOCs ranged from 23.76 ± 0.57 µg m−3 (acetone) to 92489.91 ± 0.65 µg m−3 (m,p-xylene). The predominant compounds were m,p-xylene (up to 92489.91 ± 0.65 µg m−3), ethylbenzene (up to 91188.95 ± 0.34 µg m−3), and toluene (up to 46088.84 ± 0.14 µg m−3). The non-cancer risks of benzene, n-nonane, trichloroethylene, tetrachloroethylene, xylene, and ethylbenzene surpassed the reference value in most of the sectors. In addition, total lifetime risks of cancer were in the range of 1.8 × 10−5–3.85 × 10−3, suggesting that there was a risk of carcinogenesis in all studied sections, mainly due to ethylbenzene and benzene. Considering their high exposure concentrations and their associated non-carcinogenic and carcinogenic risks, biological monitoring of workers and the use of technical and modern engineering control measures are recommended.
Comparative Assessment of Different Yeast Cell Wall-Based Mycotoxin Adsorbents Using a Model- and Bioassay-Based In Vitro Approach
Authors: Xu Ran , Yiannikouris Alexandros , Shandilya Umesh K. , Karrow Niel A.
Published: January 2023

Frequently reported occurrences of deoxynivalenol (DON), beauvericin (BEA), and, to a lesser extent, ochratoxin A (OTA) and citrinin (CIT) in ruminant feed or feedstuff could represent a significant concern regarding feed safety, animal health, and productivity. Inclusion of yeast cell wall-based mycotoxin adsorbents in animal feeds has been a common strategy to mitigate adverse effects of mycotoxins. In the present study, an in vitro approach combining adsorption isotherm models and bioassays was designed to assess the efficacy of yeast cell wall (YCW), yeast cell wall extract (YCWE), and a postbiotic yeast cell wall-based blend (PYCW) products at the inclusion rate of 0.5% (w/v) (ratio of adsorbent mass to buffer solution volume). The Hill’s adsorption isotherm model was found to best describe the adsorption processes of DON, BEA, and CIT. Calculated binding potential for YCW and YCWE using the Hill’s model exhibited the same ranking for mycotoxin adsorption, indicating that BEA had the highest adsorption rate, followed by DON and CIT, which was the least adsorbed. PYCW had the highest binding potential for BEA compared with YCW and YCWE. In contrast, the Freundlich isotherm model presented a good fit for OTA adsorption by all adsorbents and CIT adsorption by PYCW. Results indicated that YCW was the most efficacious for sequestering OTA, whereas YCWE was the least efficacious. PYCW showed greater efficacy at adsorbing OTA than CIT. All adsorbents exhibited high adsorption efficacy for BEA, with an overall percentage average of bound mycotoxin exceeding 60%, whereas moderate efficacies for the other mycotoxins were observed (up to 37%). Differences in adsorbent efficacy of each adsorbent significantly varied according to experimental concentrations tested for each given mycotoxin (p < 0.05). The cell viability results from the bioassay using a bovine mammary epithelial cell line (MAC-T) indicated that all tested adsorbents could potentially mitigate mycotoxin-related damage to bovine mammary epithelium. Results from our studies suggested that all tested adsorbents had the capacity to adsorb selected mycotoxins in vitro, which could support their use to mitigate their effects in vivo.
The Role of CD1 Gene Polymorphism in the Genetic Susceptibility to Spondyloarthropathies in the Moroccan Population and the Possible Cross-Link with Celiac Disease
Authors: Canossi Angelica , Oumhani Khadija , Del Beato Tiziana , Sebastiani Pierluigi , Colanardi Alessia , Aureli Anna
Published: January 2023

Spondyloarthropathies (SpA) are a group of chronic inflammatory disorders usually affecting the axial spine and asymmetrical peripheral joints. Strong evidence links genetic and environmental factors to SpA pathogenesis. The HLA-B27 is the most important genetic factor associated with SpA. Nevertheless, the involvement of other HLA and non-HLA loci has been also reported. Some patients with SpA may also manifest features of celiac disease (CeD), thus suggesting a genetic overlap across these autoimmune diseases. Recently, CD1 glycoproteins, a class of molecules able to bind and present non peptidic antigens to T cells, aroused interest for their contribution to the pathogenesis of CeD. Therefore, to evaluate whether functional polymorphisms of CD1A and E genes also influence susceptibility to SpA, we analyzed 86 patients from Morocco affected by SpA and 51 healthy controls, using direct sequencing analysis. An increase of CD1E*01/01 homozygous genotype (p = 0.046) was found in SpA, compared with controls. CD1E*01/01 genotype was associated particularly to patients with sacroiliac joints/spine/peripheral joints pain (p = 0.0068), while a decrease of CD1E*01/02 genotype was evidenced compared to controls (p = 0.0065). Results from haplotypes analysis demonstrated that CD1A*02-E*02 decreased the risk of SpA, while CD1A*02-E*01 increased risk to develop disease. Our data indicate a relationship between CD1 genes and susceptibility to SpA in the Moroccan population and suggest the existence of shared genetic risk loci across SpA and CeD that might be useful to explain common pathogenetic features and define novel therapeutic strategies.
Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination
Authors: Tauzin Alexandra , Benlarbi Mehdi , Medjahed Halima , Grégoire Yves , Perreault Josée , Gendron-Lepage Gabrielle , Gokool Laurie , Morrisseau Chantal , Arlotto Pascale , Tremblay Cécile , Kaufmann Daniel E. , Martel-Laferrière Valérie , Levade Inès , Côté Marceline , De Serres Gaston , Bazin Renée , Finzi Andrés
Published: January 2023

The Omicron BQ.1.1 variant is now the major SARS-CoV-2 circulating strain in many countries. Because of the many mutations present in its Spike glycoprotein, this variant is resistant to humoral responses elicited by monovalent mRNA vaccines. With the goal to improve immune responses against Omicron subvariants, bivalent mRNA vaccines have recently been approved in several countries. In this study, we measure the capacity of plasma from vaccinated individuals, before and after a fourth dose of mono- or bivalent mRNA vaccine, to recognize and neutralize the ancestral (D614G) and the BQ.1.1 Spikes. Before and after the fourth dose, we observe a significantly better recognition and neutralization of the ancestral Spike. We also observe that fourth-dose vaccinated individuals who have been recently infected better recognize and neutralize the BQ.1.1 Spike, independently of the mRNA vaccine used, than donors who have never been infected or have an older infection. Our study supports that hybrid immunity, generated by vaccination and a recent infection, induces higher humoral responses than vaccination alone, independently of the mRNA vaccine used.
Assessment of Liver Regeneration in Patients Who Have Undergone Living Donor Hepatectomy for Living Donor Liver Transplantation
Authors: Satilmis Basri , Akbulut Sami , Sahin Tevfik Tolga , Dalda Yasin , Tuncer Adem , Kucukakcali Zeynep , Ogut Zeki , Yilmaz Sezai
Published: January 2023

Background: Inflammation and the associated immune pathways are among the most important factors in liver regeneration after living donor hepatectomy. Various biomarkers, especially liver function tests, are used to show liver regeneration. The aim of this study was to evaluate the course of liver regeneration following donor hepatectomy (LDH) by routine and regeneration-related biomarkers. Method: Data from 63 living liver donors (LLDs) who underwent LDH in Inonu University Liver Transplant Institute were prospectively analyzed. Serum samples were obtained on the preoperative day and postoperative days (POD) 1, 3, 5, 10, and 21. Regenerative markers including alfa-fetoprotein (AFP), des carboxy prothrombin (DCP), ornithine decarboxylase (ODC), retinol-binding protein 4 (RBP4), and angiotensin-converting enzyme isotype II (ACEII) and liver function tests including alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP) and total bilirubin levels were all analyzed. Results: The median age of the LLDs was 29.7 years and 28 LLDs were female. Eight LLDs developed postoperative complications requiring relaparotomy. The routine laboratory parameters including AST (<0.001), ALT (<0.001), ALP (<0.001), and total bilirubin (<0.001) showed a significant increase over time until postoperative day (POD) 3. For the regeneration-related parameters, except for the RBP4, all parameters including ACEII (p = 0.006), AFP (p = 0.002), DCP (p = 0.007), and ODC (p = 0.002) showed a significant increase in POD3. The regeneration parameters showed a different pattern of change. In right-lobe liver grafts, ACEII (p = 0.002), AFP (p = 0.035), and ODC (p = 0.001) showed a significant increase over time. DCP (p = 0.129) and RBP4 (p = 0.335) showed no significant changes in right-lobe liver grafts. Conclusions: Regenerative markers are increased in a sustained fashion following LDH. This is more prominent following right-lobe grafts which are indicative of progenitor-associated liver regeneration.
Lessons from COVID’S Vaccination: External-Internal Frictions and Efficiency
Authors: Ramirez-Zamudio Aldo , Sanchez Davila Elmer
Published: January 2023

This paper explores some economic variables that determine the emerging of new COVID-19 variants and the determinants of vaccination advances in 108 countries during a quarterly period from March 2020 to March 2022. We found that more people being fully vaccinated and more education (measured as schooling years) decrease the probability of the emergence of new COVID-19 variants, but more crowded cities and higher percentages of urban population increase that probability. Furthermore, we found that the percentage of fully vaccinated people depends positively on the country’s preparation to respond to a health crisis, educational levels, and the index of economic complexity (which measures how diverse in the production of goods and services a country is and the level of its infrastructure), and it depends negatively on the percentage of rural populations (which makes vaccination more difficult).
II. La supercherie de l’assimilation, quand «assistante» signifie «sous»
Authors: Alonzo Philippe
Published: January 2023

Employé, mot à double sens. Avoir un emploi : voilà aujourd'hui le sens lourd. Mais l'autre, non moins plein, se lit au féminin : travailler comme employée. Car aujourd'hui, en France, trois employés sur quatre sont des femmes. Genre et catégorie sociale -ou genre et classe ? -, ainsi va le «petit » salariat, celui des employées et des ouvriers (ceux-ci aux 4/5 masculins). Petit salariat, le plus grand pourtant par son nombre. Ce n 'est pas là, la seule contradiction, le seul paradoxe ! Le maintien, dans des sous-statuts de salariées occasionnelles, non qualifiées, n'est pas l'apanage des «employées libre-service caissières » (ELSC.), ni même de celles qui travaillent à temps partiel. Dans les bureaux, les politiques de gestion du personnel utilisent et intègrent des dénominations de postes, dont la construction et les délimitations floues permettent de faire appel à une population sur-qualifiée, par rapport aux exigences de l'emploi à pourvoir. Ce décalage entre «le titre et le poste » place les employées qui le vivent dans des situations difficiles au regard de leur expérience quotidienne de travail. L'appellation complémentaire «aide », «assistante de... » qui au départ a pu être présentée comme la promesse d'une ascension professionnelle rapide, fait vite place à la désillusion avec le maintien dans un statut d'employée. Par leurs trajectoires communes, par l’importance des désillusions subies, les employées dont il sera question dans cette intervention, constituent le fil d'Ariane à partir duquel on se propose d'analyser la situation de ce qu’il faut bien dénommer des cadres à statut employé.
Le collectif et le particulier. Réflexions autour d'un mouvement inachevé
Authors: Cours-Salies Pierre
Published: January 2023

Addition de corporatisme ou possibilité d'objectifs collectifs ? Cette polémique, au sujet du mouvement de l'automne 1995, invite à repréciser en partie quelques conceptualisations : les rapports entre le collectif et les particuliers, la notion de classe et singulièrement de classe ouvrière. Une série d'entretiens, au cours du mouvement, permet de revisiter les relations entre conscience, objectifs collectifs et récits individuels. Nous ferons également une relecture de la notion de classe à partir de la définition qu’en donnent Flora Tristan (1843) ou la CGT d'avant 1914 (Maxime Leroy, 1913).