bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Repair Technology of Laser Damage-Fused Silica Optics Based on the Neural Network Method
Authors: Wang Bo , Zhang Wanli , Shi Feng , Song Ci , Zhang Yaofei , Sun Guoyan , Guo Shuangpeng
Published: August 2022

As a key component of a high-power laser device, fused silica optics needs to bear great laser energy, and laser damage is easily generated on the optical surface. In order to improve the service life and availability of optics, it is necessary to repair the damaged optics. In this work, the repair technique of damaged, fused silica optics was studied. The neural network method was mainly used to establish the correlation between the number of small-scale damage points and the repair depth. The prediction accuracy of the model is better than 90%. Based on the neural network model, the removal depth parameters were optimized with the suppression coefficient of the damage points. The processing effect of the optimized parameters was verified by magnetorheological polishing experiments. In this paper, a repair technique based on a neural network was proposed, which avoids the low efficiency caused by processing iterations in the repair process, and can accurately what was expected. The method proposed in this work has an important reference value in the repair process of fused silica optics.
Recycled Aggregate: A Viable Solution for Sustainable Concrete Production
Authors: Marvila Markssuel , de Matos Paulo , Rodríguez Erich , Monteiro Sergio , de Azevedo Afonso
Published: August 2022

Construction and demolition activities consume large amounts of natural resources, generating 4.5 bi tons of solid waste/year, called construction and demolition waste (C&DW) and other wastes, such as ceramic, polyethylene terephthalate (PET), glass, and slag. Furthermore, around 32 bi tons of natural aggregate (NA) are extracted annually. In this scenario, replacing NA with recycled aggregate (RA) from C&DW and other wastes can mitigate environmental problems. We review the use of RA for concrete production and draw the main challenges and outlook. RA reduces concrete’s fresh and hardened performance compared to NA, but these reductions are often negligible when the replacement levels are kept up to 30%. Furthermore, we point out efficient strategies to mitigate these performance reductions. Efforts must be spent on improving the efficiency of RA processing and the international standardization of RA.
Mid-Infrared Laser Generation of Zn1−xMnxSe and Zn1−xMgxSe (x ≈ 0.3) Single Crystals Co-Doped by Cr2+ and Fe2+ Ions—Comparison of Different Excitation Wavelengths
Authors: Říha Adam , Jelínková Helena , Doroshenko Maxim E. , Jelínek Michal , Šulc Jan , Němec Michal , Vyhlídal David , Kovalenko Nazar O.
Published: August 2022

Two different mid-infrared (mid-IR) solid-state crystalline laser active media of Cr2+, Fe2+:Zn1−xMnxSe and Cr2+, Fe2+:Zn1−xMgxSe with similar amounts of manganese or magnesium ions of x ≈ 0.3 were investigated at cryogenic temperatures for three different excitation wavelengths: Q-switched Er:YLF laser at the wavelength of 1.73 μm, Q-switched Er:YAG laser at 2.94 μm, and the gain-switched Fe:ZnSe laser operated at a liquid nitrogen temperature of 78 K at ∼4.05 μm. The temperature dependence of spectral and laser characteristics was measured. Depending on the excitation wavelength and the selected output coupler, both laser systems were able to generate radiation by Cr2+ or by Fe2+ ions under direct excitation or indirectly by the Cr2+→ Fe2+ energy transfer mechanism. Laser generation of Fe2+ ions in Cr2+, Fe2+:Zn1−xMnxSe and Cr2+, Fe2+:Zn1−xMgxSe (x ≈ 0.3) crystals at the wavelengths of ∼4.4 and ∼4.8 μm at a temperature of 78 K was achieved, respectively. The excitation of Fe2+ ions in both samples by direct 2.94 μm as well as ∼4.05 μm radiation or indirectly via the Cr2+→ Fe2+ ions’ energy transfer-based mechanism by 1.73 μm radiation was demonstrated. Based on the obtained results, the possibility of developing novel coherent laser systems in mid-IR regions (∼2.3–2.5 and ∼4.4–4.9 μm) based on AIIBVI matrices was presented.
Qualitative Differences and Emission Persistence of Volatile Organic Compounds from Bio-Based Particleboards
Authors: Tupciauskas Ramunas , Meile Kristine , Godina Daniela , Rizhikovs Janis , Syrpas Michail , Venskutonis Petras Rimantas
Published: August 2022

An attempt to reduce, replace, or even eliminate the synthetic resins from wood-based panels alongside broadening the array of raw lignocellulosics is still essential and attractive. Many pretreatments of lignocellulosics have been studied, among which steam explosion (SE) resulted in superior physical-mechanical properties of the obtained binder-less boards. However, the SE pretreatment leads to a relatively strong odor, which is even emitted from the obtained binder-less boards independent of the raw lignocellulosic, raising concern about the use of the boards. Emissions of volatile organic compounds (VOCs) were investigated in the framework of the study from binder-less boards obtained from different SE raw lignocellulosics and SE-untreated suberinic acids-bonded particleboard. VOCs were collected by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography–mass spectrometry (GC–MS) for 28 days with an interval of 2 weeks. The results showed that the number of detected VOCs and their chromatographic peak area varied significantly depending on the raw lignocellulosic, board density, and post-treatment (overlayering), decreasing over time. The lowest area of detected VOCs was demonstrated by the suberinic acids-bonded particleboard, while the highest area was detected from the high-density binder-less board obtained from SE hemp shives with the main compound of furfural (up to 70%) in all board types.
Repeated Impact Response of Normal- and High-Strength Concrete Subjected to Temperatures up to 600 °C
Authors: Abid Sallal R. , Abbass Ahmmad A. , Murali Gunasekaran , Al-Sarray Mohammed L. J. , Nader Islam A. , Ali Sajjad H.
Published: August 2022

With the aim of investigating the response of concrete to the dual effect of accidental fire high temperatures and possible induced impacts due to falling fragmented or burst parts or objects, an experimental work is conducted in this study to explore the influence of exposure to temperatures of 200, 400 and 600 °C on the responses of concrete specimens subjected to impact loads. Cylindrical specimens are tested using the recommended repeated impact procedure of the ACI 544-2R test. Three concrete mixtures with concrete nominal design strengths of 20, 40 and 80 MPa are introduced to represent different levels of concrete strength. From each concrete mixture, 24 cylinders and 12 cubes are prepared to evaluate the residual impact resistance and compressive strength. Six cylindrical specimens and three cubes from each concrete mixture are heated to each of the three levels of high temperatures, while the other six cylinders and three cubes are tested without heating as reference specimens. The test results show that the behavior of impact resistance is completely different from that of compressive strength after exposure to high temperatures; the cylindrical specimens lose more than 80% of the cracking and failure impact resistance after exposure to 200 °C, while impact resistance almost vanishes after exposure to 400 and 600 °C. Concrete compressive strength is found to be effective on the unheated impact specimens, where the higher-strength cylinders retain significantly higher impact numbers. This effect noticeably decreases after exposure to 200 and 400 °C, and vanishes after exposure to 600 °C.
The Zn1−xPbxCr2Se4—Single Crystals Obtained by Chemical Vapour Transport—Structure and Magnetic, Electrical, and Thermal Properties
Authors: Jendrzejewska Izabela , Groń Tadeusz , Kusz Joachim , Stokłosa Zbigniew , Pietrasik Ewa , Goryczka Tomasz , Sawicki Bogdan , Goraus Jerzy , Jampilek Josef , Duda Henryk , Witkowska-Kita Beata
Published: August 2022

Monocrystalline chalcogenide spinels ZnCr2Se4 are antiferromagnetic and semiconductor materials. They can be used to dope or alloy with related semiconducting spinels. Therefore, their Pb-doped display is expected to have unique properties and new potential applications. This paper presents the results of dc and ac magnetic measurements, including the critical fields visible on the magnetisation isotherms, electrical conductivity, and specific heat of the ZnCr2S4:Pb single crystals. These studies showed that substituting the diamagnetic Pb ion with a large ion radius for the Zn one leads to strong short-range ferromagnetic interactions in the entire temperature range and spin fluctuations in the paramagnetic region at Hdc = 50 kOe.
Relation of Dietary n-3 and n-6 Fatty Acid Intakes to Metabolic Syndrome in Middle-Aged People Depending on the Level of HbA1c: A Review of National Health and Nutrition Survey Data from 2014 to 2016
Authors: Park Seo-Woo , Kim Do-Yeong , Bak Gyeong-Tae , Hyun Dae-Sung , Kim Sung-Kyung
Published: August 2022

Background and Objectives: The relation of dietary n-6 fatty acid to metabolic syndrome has not been examined and clearly defined. To improve health in the general population, this study was to investigate the role of n-3 and n-6 fatty acids in the reduction in metabolic syndrome and to observe changes in the effects of these fatty acids depending on the level of insulin resistance. Materials and Methods: This cross-sectional study utilized national health and nutrition survey data from 2014 to 2016. From the data, a relation of n-3 and n-6 fatty acid intakes to metabolic syndrome and Hemoglobin A1c (HbA1c)’s role in the relation was evaluated and analyzed for 4852 patients between 40 and 64 years old. Intake frequency of 112 nutrition and daily consumption amounts were identified, and intakes of n-3 and n-4 fatty acids were calculated from this data. Metabolic syndrome was determined for each participant using diagnostic standards for the Asian population published by the National Cholesterol Education Program. Results: Among the total 4852 subjects, 1583 (32.6%) had metabolic syndrome; 736 of 1875 (39.3%) males and 847 of 2977 (28.5%) females had the syndrome. In males, when their HbA1c was low (<5.4%), intakes of both n-3 and n-6 fatty acids were related to a 43–63% decreased prevalence of metabolic syndrome with significance, and a similar negative tendency was also observed in females. On the contrary, for both males and females, no statistically significant correlation was present when HbA1c was high. Conclusion: It was considered that consistent and regular dietary intakes of n-3 and n-6 fatty acids may contribute greatly to prevent or treat metabolic syndrome in healthy males with normal insulin sensitivity, but the effect of their dietary intakes was found to be limited in a group with strong insulin resistance. The conclusion of this study presents a valuable reference and knowledge to provide nutritional education to the general population.
Changes Overtime in Perinatal Management and Outcomes of Extremely Preterm Infants in One Tertiary Care Romanian Center
Authors: Ungureanu Diana , Boghossian Nansi S. , Suciu Laura Mihaela
Published: August 2022

Background and Objectives: Extremely preterm infants were at increased risk of mortality and morbidity. The purpose of this study was to: (1) examine changes over time in perinatal management, mortality, and major neonatal morbidities among infants born at 250–286 weeks’ gestational age and cared for at one Romanian tertiary care unit and (2) compare the differences with available international data. Material and Methods: This study consisted of infants born at 250–286 weeks in one tertiary neonatal academic center in Romania during two 4-year periods (2007–2010 and 2015–2018). Major morbidities were defined as any of the following: severe intraventricular hemorrhage (IVH), severe retinopathy of prematurity (ROP), necrotizing enterocolitis (NEC), and bronchopulmonary dysplasia (BPD). Adjusted logistic regression models examined the association between the mortality and morbidity outcome and the study period. Results: The two cohorts differed with respect to antenatal antibiotics and rates of cesarean birth but had similar exposure to antenatal steroids and newborn referral to the tertiary care center. In logistic regression analyses, infants in the newer compared to the older cohort had a lower incidence of death (OR: 0.19; 95% CI: 0.11–0.35), a lower incidence of IVH (OR: 0.26; 95% CI: 0.15–0.46), and increased incidence of NEC (OR: 19.37; 95% CI: 2.41–155.11). Conclusions: Changes over time included higher use of antenatal antibiotics and cesarean delivery and no change in antenatal steroids administration. Overall mortality was lower in the newer cohort, especially for infants 250–266 weeks’ gestation, NEC was higher while BPD and ROP were not different.
Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity
Authors: Krysenko Sergii , Wohlleben Wolfgang
Published: August 2022

Nitrogen is an essential element required for bacterial growth. It serves as a building block for the biosynthesis of macromolecules and provides precursors for secondary metabolites. Bacteria have developed the ability to use various nitrogen sources and possess two enzyme systems for nitrogen assimilation involving glutamine synthetase/glutamate synthase and glutamate dehydrogenase. Microorganisms living in habitats with changeable availability of nutrients have developed strategies to survive under nitrogen limitation. One adaptation is the ability to acquire nitrogen from alternative sources including the polyamines putrescine, cadaverine, spermidine and spermine, as well as the monoamine ethanolamine. Bacterial polyamine and monoamine metabolism is not only important under low nitrogen availability, but it is also required to survive under high concentrations of these compounds. Such conditions can occur in diverse habitats such as soil, plant tissues and human cells. Strategies of pathogenic and non-pathogenic bacteria to survive in the presence of poly- and monoamines offer the possibility to combat pathogens by using their capability to metabolize polyamines as an antibiotic drug target. This work aims to summarize the knowledge on poly- and monoamine metabolism in bacteria and its role in nitrogen metabolism.
Triboelectric Nanogenerators for Harvesting Diverse Water Kinetic Energy
Authors: Cui Xiaojing , Yu Cecilia , Wang Zhaosu , Wan Dong , Zhang Hulin
Published: August 2022

The water covering the Earth’s surface not only supports life but also contains a tremendous amount of energy. Water energy is the most important and widely used renewable energy source in the environment, and the ability to extract the mechanical energy of water is of particular interest since moving water is ubiquitous and abundant, from flowing rivers to falling rain drops. In recent years, triboelectric nanogenerators (TENGs) have been promising for applications in harvesting kinetic energy from water due to their merits of low cost, light weight, simple structure, and abundant choice of materials. Furthermore, TENGs can also be utilized as self-powered active sensors for monitoring water environments, which relies on the output signals of the TENGs caused by the movement and composition of water. Here, TENGs targeting the harvest of different water energy sources have been systematically summarized and analyzed. The TENGs for harvesting different forms of water energy are introduced and divided on the basis of their basic working principles and modes, i.e., in the cases of solid–solid and solid–liquid. A detailed review of recent important progress in TENG-based water energy harvesting is presented. At last, based on recent progresses, the existing challenges and future prospects for TENG-based water energy harvesting are also discussed.
An Electrochemical Tilt Sensor with Double-Band Electrodes Fabricated by Wire Winding
Authors: Cheung Yik-Kin , Yu Hongyu
Published: August 2022

This paper presents the principle, design, fabrication, and characterization of Molecular Electronic Transducer (MET) dual-axis tilt sensors. The proposed sensor has a 3D-printed cylindrical channel inserted with four double-band electrodes and partially filled with a liquid electrolyte. The double-band electrodes were fabricated by wire winding with a ~0.1 mm anode-cathode distance under controlled tension. It allows the electrode to become any 3D coil rather than a 2D structure by microfabrication and exhibits good repeatability (±10%). The tilting changes the electrolyte level and electrode-electrolyte contact area, resulting in Faradaic current changes. The x-axis and the y-axis sensitivity reach 0.121 V/° and 0.154 V/°, respectively.
Anomalous Photocurrent Reversal Due to Hole Traps in AlGaN-Based Deep-Ultraviolet Light-Emitting Diodes
Authors: Lim Seungyoung , Kim Tae-Soo , Kang Jaesang , Kim Jaesun , Song Minhyup , Kim Hyun Deok , Song Jung-Hoon
Published: August 2022

The trap states and defects near the active region in deep-ultraviolet (DUV) light-emitting diodes (LED) were investigated through wavelength-dependent photocurrent spectroscopy. We observed anomalous photocurrent reversal and its temporal recovery in AlGaN-based DUV LEDs as the wavelength of illuminating light varied from DUV to visible. The wavelength-dependent photocurrent measurements were performed on 265 nm-emitting DUV LEDs under zero-bias conditions. Sharp near-band-edge (~265 nm) absorption was observed in addition to broad (300–800 nm) visible-range absorption peaks in the photocurrent spectrum, while the current direction of these two peaks were opposite to each other. In addition, the current direction of the photocurrent in the visible wavelength range was reversed when a certain forward bias was applied. This bias-induced current reversal displayed a slow recovery time (~6 h) when the applied forward voltage was removed. Furthermore, the recovery time showed strong temperature dependency and was faster as the sample temperature increased. This result can be consistently explained by the presence of hole traps at the electron-blocking layer and the band bending caused by piezoelectric polarization fields. The activation energy of the defect state was calculated to be 279 meV using the temperature dependency of the recovery time.
Correction: Peters et al. Benchmarking the ACEnano Toolbox for Characterisation of Nanoparticle Size and Concentration by Interlaboratory Comparison. Molecules 2021, 26, 5315
Authors: Peters Ruud , Elbers Ingrid , Undas Anna , Sijtsma Eelco , Briffa Sophie , Carnell-Morris Pauline , Siupa Agnieszka , Yoon Tae-Hyun , Burr Loïc , Schmid David , Tentschert Jutta , Hachenberger Yves , Jungnickel Harald , Luch Andreas , Meier Florian , Kocic Jovana , Kim Jaeseok , Park Byong Chon , Hardy Barry , Johnston Colin , Jurkschat Kerstin , Radnik Jörg , Hodoroaba Vasile-Dan , Lynch Iseult , Valsami-Jones Eugenia
Published: August 2022

Due to miscommunication a number of potential co-authors were not listed in the original publication [...]
Indigo Carmine in a Food Dye: Spectroscopic Characterization and Determining Its Micro-Concentration through the Clock Reaction
Authors: Pagnacco Maja C. , Maksimović Jelena P. , Nikolić Nenad T. , Bajuk Bogdanović Danica V. , Kragović Milan M. , Stojmenović Marija D. , Blagojević Stevan N. , Senćanski Jelena V.
Published: August 2022

Indigo carmine is a commonly used industrial blue dye. To determine its concentration in a commercially available food dye composed of a mixture of indigo carmine and D-glucose, this paper characterizes it through (ATR, KBr) FTIR micro-Raman as well as UV/Vis and clock: Briggs–Rauscher (BR) oscillatory reaction methods. The indigo carmine was detected in the bulk food dye only by applying micro-Raman spectroscopy, indicating a low percentage of the indigo carmine present. This research provides an improvement in the deviations from the experimental Raman spectrum as calculated by the B97D/cc-pVTZ level of theory one, resulting in a better geometrical optimization of the indigo carmine molecule compared to data within the literature. The analytical curves used to determine indigo carmine concentrations (and quantities) in an aqueous solution of food dye were applied by means of UV/Vis and BR methods. BR yielded significantly better analytical parameters: 100 times lower LOD and LOQ compared to commonly used UV/Vis. The remarkable sensitivity of the BR reaction towards indigo carmine suggests that not only does indigo carmine react in an oscillatory reaction but also its decomposition products, meaning that the multiple oxidation reactions have an important role in the BR’s indigo carmine mechanism. The novelty of this research is the investigation of indigo carmine using a clock BR reaction, opening new possibilities to determine indigo carmine in other complex samples (pharmaceutical, food, etc.).
Corrosion Inhibition of Mild Steel in Hydrochloric Acid Environment Using Terephthaldehyde Based on Schiff Base: Gravimetric, Thermodynamic, and Computational Studies
Authors: Mahdi Bahaa Sami , Abbass Muna Khethier , Mohsin Mustafa Khudhair , Al-azzawi Waleed Khalid , Hanoon Mahdi M. , Al-kaabi Mohammed Hliyil Hafiz , Shaker Lina M. , Al-amiery Ahmed A. , Isahak Wan Nor Roslam Wan , Kadhum Abdul Amir H. , Takriff Mohd S.
Published: August 2022

Using traditional weight-loss tests, as well as different electrochemical techniques (potentiodynamic polarization and electrochemical impedance spectroscopy), we investigated the corrosion-inhibition performance of 2,2′-(1,4-phenylenebis(methanylylidene)) bis(N-(3-methoxyphenyl) hydrazinecarbothioamide) (PMBMH) as an inhibitor for mild steel in a 1 M hydrochloric acid solution. The maximum protection efficacy of 0.0005 M of PMBMH was 95%. Due to the creation of a protective adsorption layer instead of the adsorbed H2O molecules and acidic chloride ions, the existence of the investigated inhibitor reduced the corrosion rate and increased the inhibitory efficacy. The inhibition efficiency increased as the inhibitor concentration increased, but it decreased as the temperature increased. The PMBMH adsorption mode followed the Langmuir adsorption isotherm, with high adsorption-inhibition activity. Furthermore, the value of the ∆Gadso  indicated that PMBMH contributed to the physical and chemical adsorption onto the mild-steel surface. Moreover, density functional theory (DFT) helped in the calculation of the quantum chemical parameters for finding the correlation between the inhibition activity and the molecular structure. The experimental and theoretical findings in this investigation are in good agreement.
Biofilm-i: A Platform for Predicting Biofilm Inhibitors Using Quantitative Structure—Relationship (QSAR) Based Regression Models to Curb Antibiotic Resistance
Authors: Rajput Akanksha , Bhamare Kailash T. , Thakur Anamika , Kumar Manoj
Published: August 2022

Antibiotic drug resistance has emerged as a major public health threat globally. One of the leading causes of drug resistance is the colonization of microorganisms in biofilm mode. Hence, there is an urgent need to design novel and highly effective biofilm inhibitors that can work either synergistically with antibiotics or individually. Therefore, we have developed a recursive regression-based platform “Biofilm-i” employing a quantitative structure–activity relationship approach for making generalized predictions, along with group and species-specific predictions of biofilm inhibition efficiency of chemical(s). The platform encompasses eight predictors, three analysis tools, and data visualization modules. The experimentally validated biofilm inhibitors for model development were retrieved from the “aBiofilm” resource and processed using a 10-fold cross-validation approach using the support vector machine and andom forest machine learning techniques. The data was further sub-divided into training/testing and independent validation sets. From training/testing data sets the Pearson’s correlation coefficient of overall chemicals, Gram-positive bacteria, Gram-negative bacteria, fungus, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Escherichia coli was 0.60, 0.77, 0.62, 0.77, 0.73, 0.83, 0.70, and 0.71 respectively via Support Vector Machine. Further, all the QSAR models performed equally well on independent validation data sets. Additionally, we also checked the performance of the random forest machine learning technique for the above datasets. The integrated analysis tools can convert the chemical structure into different formats, search for a similar chemical in the aBiofilm database and design the analogs. Moreover, the data visualization modules check the distribution of experimentally validated biofilm inhibitors according to their common scaffolds. The Biofilm-i platform would be of immense help to researchers engaged in designing highly efficacious biofilm inhibitors for tackling the menace of antibiotic drug resistance.
CeO2 Nanoparticle-Loaded MnO2 Nanoflowers for Selective Catalytic Reduction of NOx with NH3 at Low Temperatures
Authors: Li Shun , Zheng Zuquan , Zhao Zhicheng , Wang Youling , Yao Yao , Liu Yong , Zhang Jianming , Zhang Zuotai
Published: August 2022

CeO2 nanoparticle-loaded MnO2 nanoflowers, prepared by a hydrothermal method followed by an adsorption-calcination technique, were utilized for selective catalytic reduction (SCR) of NOx with NH3 at low temperatures. The effects of Ce/Mn ratio and thermal calcination temperature on the NH3–SCR activity of the CeO2-MnO2 nanocomposites were studied comprehensively. The as-prepared CeO2-MnO2 catalysts show high NOx reduction efficiency in the temperature range of 150–300 °C, with a complete NOx conversion at 200 °C for the optimal sample. The excellent NH3–SCR performance could be ascribed to high surface area, intimate contact, and strong synergistic interaction between CeO2 nanoparticles and MnO2 nanoflowers of the well-designed composite catalyst. The in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTs) characterizations evidence that the SCR reaction on the surface of the CeO2-MnO2 nanocomposites mainly follows the Langmuir–Hinshelwood (L-H) mechanism. Our work provides useful guidance for the development of composite oxide-based low temperature NH3–SCR catalysts.
Light and Potassium Improve the Quality of Dendrobium officinale through Optimizing Transcriptomic and Metabolomic Alteration
Authors: Jia Yue , Liu Juan , Xu Mengyao , Chen Guihong , Tan Mingpu , Xiang Zengxu
Published: August 2022

Background: Dendrobium officinale is a perennial epiphytic herb in Orchidaceae. Cultivated products are the main alternative for clinical application due to the shortage of wild resources. However, the phenotype and quality of D. officinale have changed post-artificial cultivation, and environmental cues such as light, temperature, water, and nutrition supply are the major influencing factors. This study aims to unveil the mechanisms beneath the cultivation-induced variation by analyzing the changes of the metabolome and transcriptome of D. officinale seedlings treated with red- blue LED light and potassium fertilizer. Results: After light- and K-treatment, the D. officinale pseudobulbs turned purple and the anthocyanin content increased significantly. Through wide-target metabolome analysis, compared with pseudobulbs in the control group (P), the proportion of flavonoids in differentially-accumulated metabolites (DAMs) was 22.4% and 33.5% post light- and K-treatment, respectively. The gene modules coupled to flavonoids were obtained through the coexpression analysis of the light- and K-treated D. officinale transcriptome by WGCNA. The KEGG enrichment results of the key modules showed that the DEGs of the D. officinale pseudobulb were enriched in phenylpropane biosynthesis, flavonoid biosynthesis, and jasmonic acid (JA) synthesis post-light- and K-treatment. In addition, anthocyanin accumulation was the main contribution to the purple color of pseudobulbs, and the plant hormone JA induced the accumulation of anthocyanins in D. officinale. Conclusions: These results suggested that light and potassium affected the accumulation of active compounds in D. officinale, and the gene-flavone network analysis emphasizes the key functional genes and regulatory factors for quality improvement in the cultivation of this medicinal plant.
Multiple Direct Effects of the Dietary Protoalkaloid N-Methyltyramine in Human Adipocytes
Authors: Carpéné Christian , Viana Pénélope , Fontaine Jessica , Laurell Henrik , Grolleau Jean-Louis
Published: August 2022

Dietary amines have been the subject of a novel interest in nutrition since the discovery of trace amine-associated receptors (TAARs), especially TAAR-1, which recognizes tyramine, phenethylamine, tryptamine, octopamine, N-methyltyramine (NMT), synephrine, amphetamine and related derivatives. Alongside the psychostimulant properties of TAAR-1 ligands, it is their ephedrine-like action on weight loss that drives their current consumption via dietary supplements advertised for ‘fat-burning’ properties. Among these trace amines, tyramine has recently been described, at high doses, to exhibit an antilipolytic action and activation of glucose transport in human adipocytes, i.e., effects that are facilitating lipid storage rather than mobilization. Because of its close structural similarity to tyramine, NMT actions on human adipocytes therefore must to be reevaluated. To this aim, we studied the lipolytic and antilipolytic properties of NMT together with its interplay with insulin stimulation of glucose transport along with amine oxidase activities in adipose cells obtained from women undergoing abdominal surgery. NMT activated 2-deoxyglucose uptake when incubated with freshly isolated adipocytes at 0.01–1 mM, reaching one-third of the maximal stimulation by insulin. However, when combined with insulin, NMT limited by half the action of the lipogenic hormone on glucose transport. The NMT-induced stimulation of hexose uptake was sensitive to inhibitors of monoamine oxidases (MAO) and of semicarbazide-sensitive amine oxidase (SSAO), as was the case for tyramine and benzylamine. All three amines inhibited isoprenaline-induced lipolysis to a greater extent than insulin, while they were poorly lipolytic on their own. All three amines—but not isoprenaline—interacted with MAO or SSAO. Due to these multiple effects on human adipocytes, NMT cannot be considered as a direct lipolytic agent, potentially able to improve lipid mobilization and fat oxidation in consumers of NMT-containing dietary supplements.
Nutri-Score: Awareness, Perception and Self-Reported Impact on Food Choices among French Adolescents
Authors: Ducrot Pauline , Julia Chantal , Serry Anne-Juliette
Published: August 2022

To date, no studies have evaluated the appropriation of the front-of-pack Nutri-Score labeling among adolescents, although they are both consumers and buyers of food products. Therefore, the objectives of the present study were (1) to assess Nutri-Score awareness, perception and self-reported impact on food choices in French adolescents and (2) to identify the determinants associated with higher Nutri-Score awareness and self-reported impact on food choices. A web-based survey was conducted in November 2021 among 1201 adolescents. Multivariate logistic models were used to evaluate the relationships between individual factors and Nutri-Score awareness and self-reported impact on food choices. Almost all the adolescents reported to know the Nutri-Score (97.0%) and more than 9 out of 10 considered this logo easy to understand and easy to identify on food packages. Finally, 54% self-reported that the label had already impacted their food choices. Girls (2.28 (1.09–4.77), p = 0.028) and the 15–17-year-olds (3.12 (1.32–7.35), p = 0.0094) were more likely to be aware of the label compared with their respective counterparts (i.e., boys and the 11–14-year-olds). Regarding the impact of food choices, the use of the Nutri-Score by the parents was the most determinant criterion (7.74 (5.74–10.42), p < 0.0001). Thus, promotion campaigns should target both adolescents and parents.
Smoking Exposure Is Associated with Serum Vitamin D Deficiency in Children: Evidence from the Japan Environment and Children’s Study
Authors: Yang Limin , Sato Miori , Saito-Abe Mayako , Miyaji Yumiko , Sato Chikako , Nishizato Minaho , Kumasaka Natsuhiko , Mezawa Hidetoshi , Yamamoto-Hanada Kiwako , Ohya Yukihiro
Published: August 2022

Tobacco smoke exposure is known to lower serum 25-hydroxyvitamin D (25(OH)D) concentrations. This study evaluated the association between passive smoking and vitamin D deficiency (VDD) in young children using data from the Japan Environment and Children’s Study (JECS), the largest birth cohort study in Japan. Information on parental smoking status was extracted from a survey of JECS for children aged 1.5 years and data for serum 25(OH)D concentrations were obtained from blood tests in the Sub-Cohort Study of JECS performed at age 2 years. Logistic regression and linear models were fitted to evaluate the association between these variables. Data were analyzed for 4593 children. After adjusting for covariates, smoke exposure was significantly associated with increased incidence of VDD (OR 1.35; 95% CI, 1.14–1.59) according to the logistic model. The linear model indicated that passive smoking negatively predicted de-seasonalized serum 25(OH)D concentrations (β −0.5; 95% CI −0.95 to −0.08) in children aged 2 years. The results suggest that smoke exposure is a risk factor for VDD in children. Given that VD plays a crucial role in bone metabolism and the immune system, our findings are significant for clinical and public health.
Hesperidin Improves Memory Function by Enhancing Neurogenesis in a Mouse Model of Alzheimer’s Disease
Authors: Lee Danbi , Kim Namkwon , Jeon Seung Ho , Gee Min Sung , Ju Yeon-Joo , Jung Min-Ji , Cho Jae Seok , Lee Yeongae , Lee Sangmin , Lee Jong Kil
Published: August 2022

Alzheimer’s disease (AD) is an irreversible neurodegenerative disease characterized by memory and cognitive impairments. Neurogenesis, which is related to memory and cognitive function, is reduced in the brains of patients with AD. Therefore, enhancing neurogenesis is a potential therapeutic strategy for neurodegenerative diseases, including AD. Hesperidin (HSP), a bioflavonoid found primarily in citrus plants, has anti-inflammatory, antioxidant, and neuroprotective effects. The objective of this study was to determine the effects of HSP on neurogenesis in neural stem cells (NSCs) isolated from the brain of mouse embryos and five familial AD (5xFAD) mice. In NSCs, HSP significantly increased the proliferation of NSCs by activating adenosine monophosphate (AMP)-activated protein kinase (AMPK)/cAMP-response element-binding protein (CREB) signaling, but did not affect NSC differentiation into neurons and astrocytes. HSP administration restored neurogenesis in the hippocampus of 5xFAD mice via AMPK/brain-derived neurotrophic factor/tropomyosin receptor kinase B/CREB signaling, thereby decreasing amyloid-beta accumulation and ameliorating memory dysfunction. Collectively, these preclinical findings suggest that HSP is a promising candidate for the prevention and treatment of AD.
Salt-Sensitive Ileal Microbiota Plays a Role in Atrial Natriuretic Peptide Deficiency-Induced Cardiac Injury
Authors: Li Siqi , Chen Sishuo , Nie Min , Wen Lijing , Zou Bin , Zhang Lingyu , Xie Jingzhou , Ser Hooi-Leng , Lee Learn-Han , Wang Shunyi , Lin Caixia , Pathak Janak L. , Zhou Weijie , Miao Ji , Wang Lijing , Zheng Lingyun
Published: August 2022

Atrial natriuretic peptide (ANP) activity deficiency contributes to salt-sensitive hypertension in humans and mice. However, the role of ileal microbiota in salt sensitivity in ANP deficiency-related cardiac injury has not been investigated yet. This study used ANP−/− mice to analyze the role of the salt-sensitive ileal microbiome on cardiac injury. ANP−/− mice showed an increase in blood pressure (BP), the heart weight/body weight (HW/BW) ratio, and cardiac hypertrophy compared with wild-type (WT) mice. ANP deficiency did not impact the histological structure but reduced occludin expression in the ileum. Antibiotics significantly relieved BP and cardiac hypertrophy in ANP−/− mice. A high-salt diet (HSD) increased BP, the HW/BW ratio, and cardiac hypertrophy/fibrosis in WT and ANP−/− mice, and an HSD treatment in ANP−/− mice exacerbated these cardiac parameters. The HSD markedly decreased muscularis layer thickening, villus length, and numbers of Paneth and goblet cells in the ileum of WT and ANP−/− mice. Furthermore, the HSD increased the level of TLR4 and IL-1β in ANP−/− mice ileum compared with WT mice. Antibiotics reduced the HW/BW ratio, cardiac hypertrophy/fibrosis, and the level of TLR4 and IL-1β in the ileum, and rescued the muscularis layer thickening, villus length, and numbers of Paneth and goblet cells in the ileum of HSD-ANP−/− mice. Importantly, ANP deficiency induced the colonization of Burkholderiales bacterium YL45, Lactobacillus johnsonii, and Lactobacillus reuteri in the ileum on the NSD diet, which was only observed in HSD-induced WT mice but not in WT mice on the NSD. Besides, the HSD significantly enhanced the sum of the percentage of the colonization of Burkholderiales bacterium YL45, Lactobacillus johnsonii, and Lactobacillus reuteri in the ileum of ANP−/− mice. Ileal microbiota transfer (IMT) from ANP−/− mice to healthy C57BL/6J mice drove Lactobacillus johnsonii and Lactobacillus reuteri colonization in the ileum, which manifested an increase in BP, the HW/BW ratio, cardiac hypertrophy, and ileal pathology compared with IMT from WT mice. The HSD in C57BL/6J mice with IMT from ANP−/− mice drove the colonization of Burkholderiales bacterium YL45, Lactobacillus johnsonii, and Lactobacillus reuteri in the ileum and further exacerbated the cardiac and ileal pathology. Our results suggest that salt-sensitive ileal microbiota is probably related to ANP deficiency-induced cardiac injury.
Association of Body Mass Index and Plant-Based Diet with Cognitive Impairment among Older Chinese Adults: A Prospective, Nationwide Cohort Study
Authors: Liang Fang , Fu Jialin , Turner-McGrievy Gabrielle , Wang Yechuang , Qiu Nan , Ding Kai , Zeng Jing , Moore Justin , Li Rui
Published: August 2022

To examine the association of body mass index (BMI) and a plant-based diet (PBD) with cognitive impairment in older adults, this cohort study used data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), a national, community-based, longitudinal, prospective study in China. Cognitive function was evaluated via the Mini-Mental State Examination (MMSE). Diet was assessed using a simplified food frequency questionnaire (FFQ), and PBD patterns were estimated using the overall plant-based diet index (PDI), the healthful plant-based diet index (hPDI), and the unhealthful plant-based diet index (uPDI). BMI was measured objectively during the physical examination. Cox proportional hazard models and restricted cubic spline analyses were used. A total of 4792 participants with normal cognition at baseline were included, and 1077 participants were identified as having developed cognitive impairment during the 24,156 person-years of follow-up. A reverse J-shaped association was observed between BMI and cognitive impairment (p = 0.005 for nonlinearity). Participants who were overweight (HR = 0.79; 95% CI 0.66–0.95) and obese (HR = 0.72; 95% CI 0.54–0.96) had a decreased risk of cognitive impairment, while those who were underweight (HR = 1.42; 95% CI 1.21–1.66) had an increased risk. Lower PDI, lower hPDI, and higher uPDI were associated with an increased risk of cognitive impairment (HR = 1.32; 95% CI 1.16–1.50 for PDI; HR = 1.46; 95% CI 1.29–1.66 for hPDI; HR = 1.21; 95% CI 1.06–1.38 for uPDI). The protective effect of being overweight on cognitive impairment was more pronounced among participants with a higher PDI (HR = 0.74; 95% CI 0.57–0.95) than those with a lower PDI (HR = 0.87; 95% CI 0.67–1.12), among participants with a higher hPDI (HR = 0.73; 95% CI 0.57–0.94) than those with a lower hPDI (HR = 0.93; 95% CI 0.72–1.10), and among participants with a lower uPDI (HR = 0.61; 95% CI 0.46–0.80) than those with a higher uPDI (HR = 1.01; 95% CI 0.80–1.27). Our results support the positive associations of overweight status, obesity, an overall PBD, and a healthful PBD with cognitive function in older adults. A lower adherence to an overall PBD, a healthful PBD, and a higher adherence to an unhealthful PBD may attenuate the protective effect of being overweight on cognitive function.
Description of a Hybrid Mindfulness-Integrated Multidisciplinary Workplace Weight Management Intervention Module ‘Mind-SLIMSHAPE’ Using the TIDieR Checklist
Authors: Abdul Basir Siti Munirah , Abdul Manaf Zahara , Mazri Fatin Hanani , Mat Ludin Arimi Fitri , Shahar Suzana , Abdul Manaf Mohd Rizal
Published: August 2022

Published reports of workplace-based weight management interventions are often poorly described and are focused on dietary, physical, and behavioral management. These strategies are often unsustainable and only have short-term effectiveness. The Mind-SLIMSHAPETM is a mindfulness-integrated multidisciplinary intervention developed to address overweight and obesity problems among desk-bound employees while improving weight-related behavior through mindfulness meditation and mindful eating exercises. The integration of mindfulness and mindful eating aims to improve the individual’s focus on the present and heighten their sensitivity towards internal and external eating cues. The aim of this article is to describe the Mind-SLIMSHAPETM intervention program using The Template for Intervention Description and Replication (TIDieR) checklist. The Mind-SLIMSHAPETM module is a 24-week intervention program that was delivered in a quasi-experimental study among employees with BMI ≥ 25.0 kg/m² in a selected higher learning institution. The module was delivered via hybrid sessions that included both face-to-face and virtual online sessions. The novelty of our description includes summaries of each intervention component with its intensity, details of the theory grounded for this program, and the rationale for the intervention components. The Mind-SLIMSHAPETM module is ready to be implemented and replicated in a similar setting with possible refinement and enhancement of the mindfulness and mindful eating elements.
Vitamin D, Vitamin D-Binding Proteins, and VDR Polymorphisms in Individuals with Hyperglycaemia
Authors: Erasmus Rajiv , Maepa Setjie , Machingura Ian , Davids Saarah , Raghubeer Shanel , Matsha Tandi
Published: August 2022

Vitamin D reportedly plays an important role in the pathogenesis of diabetes mellitus; however, this role is unclear and debated. This study investigated the association between 25(OH) vitamin D, vitamin D-binding proteins, and vitamin D receptor (VDR) polymorphisms in healthy individuals and those with prediabetes and type 2 diabetes mellitus (T2D) from South Africa. A cross-sectional study was conducted involving subjects of mixed ancestry aged ≥20 years. Males presented with higher mean 25(OH) vitamin D levels than females, while females exhibited significantly higher serum vitamin D-binding protein levels. Significant differences in mean 25(OH) vitamin D levels were observed in normo-glycaemic, prediabetes, screen-detected DM, and known DM individuals. Vitamin D receptor SNPs Fok1 and Taq1 were not associated with glycaemic status. Fok1 was not associated with 25(OH) vitamin D deficiency, while Taq1 was associated with vitamin D insufficiency. This study showed a high prevalence of vitamin D deficiency/insufficiency in this South African population, with decreased vitamin D levels observed in hyperglycaemic individuals, which was not linked to either vitamin D-binding protein or polymorphisms in Fok1 of the VDR gene. These results may be used as a platform for further research into diagnosis and treatment of hyperglycaemia.
Sperm as a Carrier of Genome Instability in Relation to Paternal Lifestyle and Nutritional Conditions
Authors: Punjabi Usha , Goovaerts Ilse , Peeters Kris , Van Mulders Helga , De Neubourg Diane
Published: August 2022

Endogenous and exogenous factors can severely affect the integrity of genetic information by inducing DNA damage and impairing genome stability. The extent to which men with and without subfertility are exposed to several adverse lifestyle factors and the impact on sperm DNA fragmentation (SDF), sperm chromatin maturity (condensation and decondensation), stability (hypo- and hypercondensation) and sperm aneuploidy are assessed in this study. Standardized assays employing flow cytometry were used to detect genome instability in 556 samples. Semen parameters deteriorated with age, BMI, increased physical activity and smoking. Age and BMI were associated with increased SDF. Increased BMI was associated with increased hypocondensed chromatin and decreased decondensed chromatin. Increase in age also caused an increase in sex chromosome aneuploidy in sperms. Surprisingly, alcohol abuse reduced chromatin hypercondensation and drug abuse reduced SDF. Although genome instability was more pronounced in the subfertile population as compared to the fertile group, the proportion of men with at least one lifestyle risk factor was the same in both the fertile and subfertile groups. While one in three benefited from nutritional supplementation, one in five showed an increase in SDF after supplementation. Whilst the message of ‘no smoking, no alcohol, no drugs, but a healthy diet’ should be offered as good health advice, we are a long way from concluding that nutritional supplementation would be beneficial for male fertility.
Rethinking Responsive Feeding: Insights from Bangladesh
Authors: Black Maureen M. , Tofail Fahmida , Hodges Eric A. , Bann Carla M. , Hamadani Jena D. , Aktar Shirina , Lutter Chessa K.
Published: August 2022

Young children’s growth is influenced by food and feeding behavior. Responsive feeding has been shown to promote healthy growth and development, to prevent under- and overfeeding, and to encourage children’s self-regulation. However, most measures of responsive feeding do not incorporate bidirectional mother-infant responsivity or early learning principles and have not been validated against observations. To overcome these gaps, we laid the groundwork for a responsive feeding measure based on a community sample of 67 mothers and their 6–18-month-old children in Bangladesh. Children were weighed and measured. Mothers reported on their child’s dietary intake and responded to a 38-item responsive feeding questionnaire developed through a 2-phase Delphi procedure. Based on a video-recorded feeding observation, mother-child dyads were categorized into proximal (43%) and distal (57%) responsivity groups. Using stepwise logistic regression, a 9-item model from the responsive feeding questionnaire had excellent fit (AUC = 0.93), sensitivity (90%), specificity (89%), positive predictive value (87%), and negative predictive value (93%). Proximal responsivity was characterized by maternal concerns about children’s dietary intake. Distal responsivity was characterized by maternal perception of children’s happy mood during feeding. Findings support responsive feeding as modulating between proximal and distal responsivity, promoting autonomy, self-regulation, and enabling children to acquire and practice healthy eating behaviors.