bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Disturbance on Understory Vegetation across Slovenian Forest Ecosystems
Authors: Kutnar Lado , Nagel Thomas A. , Kermavnar Janez
Published: November 2019

The herbaceous understory represents a key component of forest biodiversity across temperate forests of Europe. Here, we quantified changes in the diversity and composition of the forest understory layer in representative Slovenian forest ecosystems between 2004/05 and 2014/15. In total, 60 plots were placed across 10 different managed forest types, ranging from lowland deciduous and mid-altitude mesic mixed forests to mountain conifer forests. This network is part of an international network of sites launched within the ICP Forests Programme aimed to assess the condition of forests in Europe. To examine how disturbance influenced understory dynamics, we estimated the disturbance impacts considering both natural and/or anthropogenic disturbances that cause significant damage to trees and to ground-surface layers, including ground-vegetation layers and upper-soil layers. Species richness across 10 sites (gamma diversity) significantly decreased from 272 to 243 species during the study period, while mean species richness per site did not significantly change. The mean value of site level Shannon diversity indices and evenness significantly increased. The cover of most common plant species increased during the monitoring period. The mean value of disturbance estimates per site increased from 0.8% in 2004/05 (ranging from 0% to 2.5%) to 16.3% in 2014/15 (ranging from 5.0% to 38.8%), which corresponded to a reduction in total vegetation cover, including tree-layer cover. More disturbed sites showed larger temporal changes in species composition compared to less disturbed sites, suggesting that forest disturbances caused understory compositional shifts during the study period. Rather than observing an increase in plant diversity due to disturbance, our results suggest a short-term decrease in species number, likely driven by replacement of more specialized species with common species.
Analysis of Soybean Somatic Embryogenesis Using Chromosome Segment Substitution Lines and Transcriptome Sequencing
Authors: Li Si-Nan , Cheng Peng , Bai Yun-Qi , Shi Yan , Yu Jing-Yao , Li Rui-Chao , Zhou Run-Nan , Zhang Zhan-Guo , Wu Xiao-Xia , Chen Qing-Shan
Published: November 2019

Soybean is an important cash crop that is widely used as a source of vegetable protein and edible oil. The regeneration ability of soybean directly affects the application of biotechnology. In this study, we used the exogenous hormone 2,4-D to treat immature embryos. Different levels of somatic incidence were selected from the chromosome segment substitution lines (CSSLs) constructed by SN14 and ZYD00006. Transcriptome sequencing of extreme materials was performed, and 2666 differentially expressed genes were obtained. At the same time, a difference table was generated by combining the data on CSSL rearrangement. In the extreme materials, a total of 93 differentially expressed genes were predicted and were then analyzed by cluster analysis and Gene Ontology (GO) annotation. After screening and annotating the target genes, three differentially expressed genes with hormone pathways were identified. The expression patterns of the target genes were verified by real-time quantitative PCR (qRT-PCR). Haplotype polymorphism detection and linkage disequilibrium analysis were performed on the candidate gene Glyma.09g248200. This study provided more information on the regulation network of soybean somatic embryogenesis and regeneration processes, and further identified important genes in the soybean regeneration process and provided a theoretical basis for accelerating the application of biotechnology to soybean for improving its breeding efficiency.
Current and Future Concepts for the Treatment of Impaired Fracture Healing
Authors: Schlickewei Carsten W. , Kleinertz Holger , Thiesen Darius M. , Mader Konrad , Priemel Matthias , Frosch Karl-Heinz , Keller Johannes
Published: November 2019

Bone regeneration represents a complex process, of which basic biologic principles have been evolutionarily conserved over a broad range of different species. Bone represents one of few tissues that can heal without forming a fibrous scar and, as such, resembles a unique form of tissue regeneration. Despite a tremendous improvement in surgical techniques in the past decades, impaired bone regeneration including non-unions still affect a significant number of patients with fractures. As impaired bone regeneration is associated with high socio-economic implications, it is an essential clinical need to gain a full understanding of the pathophysiology and identify novel treatment approaches. This review focuses on the clinical implications of impaired bone regeneration, including currently available treatment options. Moreover, recent advances in the understanding of fracture healing are discussed, which have resulted in the identification and development of novel therapeutic approaches for affected patients.
Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation
Authors: Monteiro Rodolpho R. C. , Neto Davino M. Andrade , Fechine Pierre B. A. , Lopes Ada A. S. , Gonçalves Luciana R. B. , dos Santos José C. S. , de Souza Maria C. M. , Fernandez-Lafuente Roberto
Published: November 2019

The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.
HSF1Base: A Comprehensive Database of HSF1 (Heat Shock Factor 1) Target Genes
Authors: Kovács Dániel , Sigmond Tímea , Hotzi Bernadette , Bohár Balázs , Fazekas Dávid , Deák Veronika , Vellai Tibor , Barna János
Published: November 2019

HSF1 (heat shock factor 1) is an evolutionarily conserved master transcriptional regulator of the heat shock response (HSR) in eukaryotic cells. In response to high temperatures, HSF1 upregulates genes encoding molecular chaperones, also called heat shock proteins, which assist the refolding or degradation of damaged intracellular proteins. Accumulating evidence reveals however that HSF1 participates in several other physiological and pathological processes such as differentiation, immune response, and multidrug resistance, as well as in ageing, neurodegenerative demise, and cancer. To address how HSF1 controls these processes one should systematically analyze its target genes. Here we present a novel database called HSF1Base (hsf1base.org) that contains a nearly comprehensive list of HSF1 target genes identified so far. The list was obtained by manually curating publications on individual HSF1 targets and analyzing relevant high throughput transcriptomic and chromatin immunoprecipitation data derived from the literature and the Yeastract database. To support the biological relevance of HSF1 targets identified by high throughput methods, we performed an enrichment analysis of (potential) HSF1 targets across different tissues/cell types and organisms. We found that general HSF1 functions (targets are expressed in all tissues/cell types) are mostly related to cellular proteostasis. Furthermore, HSF1 targets that are conserved across various animal taxa operate mostly in cellular stress pathways (e.g., autophagy), chromatin remodeling, ribosome biogenesis, and ageing. Together, these data highlight diverse roles for HSF1, expanding far beyond the HSR.
Allergic and Mixed Rhinitis: Diagnosis and Natural Evolution
Authors: Greiwe Justin C. , Bernstein Jonathan A.
Published: November 2019

Chronic rhinitis (CR) is divided into two main categories: allergic rhinitis (AR) and nonallergic rhinitis (NAR). These conditions are more recognizable to an experienced clinician, as they can be more clearly demarcated diagnostically. However, an additional 30% to 50% of patients with CR might have an overlap of NAR and AR, referred to as mixed rhinitis (MR). Progress in elucidating the pathophysiologic mechanisms behind MR and NAR has been made in the past several years, and there are now several guidelines published to assist the clinician in accurately diagnosing AR, NAR, and MR. Clinical history and subjective symptoms can provide clues for differentiating AR from MR and NAR, but allergy testing is recommended to confirm these conditions. Progress in accurately diagnosing patients with CR will be made as studies incorporate subjective (i.e., validated questionnaires such as the irritant index questionnaire (IIQ), medication responsiveness, and quality-of-life tools) and objective (i.e., nasal cytologic testing, nasal provocation, and biomarkers) methods characterizing rhinitis subtypes.
Retinal Nerve Fiber Layer Thickness and Higher Relapse Frequency May Predict Poor Recovery after Optic Neuritis in MS Patients
Authors: Chisari Clara Grazia , Toro Mario Damiano , Cimino Vincenzo , Rejdak Robert , Luca Maria , Rapisarda Laura , Avitabile Teresio , Posarelli Chiara , Rejdak Konrad , Reibaldi Michele , Zappia Mario , Patti Francesco
Published: November 2019

Optic neuritis (ON) is a common manifestation of multiple sclerosis (MS). Aiming to evaluate the retinal nerve fiber layer (RNFL) with optical coherence tomography (OCT), patients with relapsing-remitting (RR) MS experiencing ON were consecutively enrolled. RNFL, ganglion cell layer (GCL), foveal thickness, and macular volume were evaluated in both the ON and unaffected (nON) eye within six days from the relapse onset (T0) and after six months (T1). Ninety patients were enrolled. At T0, ON eyes showed a significantly increased RNFL when compared to the nON eyes (129.1 ± 19.5 vs. 100.5 ± 10.1, p < 0.001). At T1 versus T0, the ON eyes showed a thinner RNFL (129.1 ± 19.5 vs. 91.6 ± 20.2, p < 0.001) and a significantly decreased GCL (80.4 ± 8.8 vs. 73.8 ± 11.6; p < 0.005). No differences were found in the nON group in retinal parameters between T0 and T1. A multivariate logistic regression analysis showed that a higher number of relapses (not ON) and a greater swelling of RNFL at T0 were associated with poor recovery. The assessment of RNFL through OCT during and after ON could be used to predict persistent visual disability.
Optimal Design of Patient-Specific Total Knee Arthroplasty for Improvement in Wear Performance
Authors: Koh Yong-Gon , Jung Kyung-Hwan , Hong Hyoung-Taek , Kim Kang-Min , Kang Kyoung-Tak
Published: November 2019

Life expectancy is on the rise and, concurrently, the demand for total knee arthroplasty (TKA), which lasts a lifetime, is increasing. To meet this demand, improved TKA designs have been introduced. Recent advances in radiography and manufacturing techniques have enabled the production of patient-specific TKA. Nevertheless, concerns regarding the wear performance, which limit the lifespan of TKA, remain to be addressed. This study aims at reducing the wear in patient-specific TKA using design optimization and parametric three-dimensional (3D) finite-element (FE) modelling. The femoral component design was implemented in a patient-specific manner, whereas the tibial insert conformity remained to be determined by design variables. The gait cycle loading condition was applied, and the optimized model was validated by the results obtained from the experimental wear tests. The wear predictions were iterated for five million gait cycles using the computational model with force-controlled input. Similar patterns for internal/external rotation and anterior/posterior translation were observed in both initial and optimal models. The wear rates for initial and optimal models were recorded as 23.2 mm3/million cycles and 16.7 mm3/million cycles, respectively. Moreover, the experimental wear rate in the optimal design was 17.8 mm3/million cycles, which validated our optimization procedure. This study suggests that tibial insert conformity is an important factor in influencing the wear performance of patient-specific TKA, and it is capable of providing improved clinical results through enhanced design selections. This finding can boost the future development of patient-specific TKA, and it can be extended to other joint-replacement designs. However, further research is required to explore the potential clinical benefits of the improved wear performance demonstrated in this study.
Organically Modified Nanoclay Filled Thin-Film Nanocomposite Membranes for Reverse Osmosis Application
Authors: Zaidi Syed , Fadhillah Farid , Saleem Haleema , Hawari Alaa , Benamor Abdelbaki
Published: November 2019

This study validates, for the first time, the effectiveness of two nanoclays, that is, cloisite (CS)-15A and montmorillonite (MNT) at the polyamide (PA) active layer in the reverse osmosis (RO) membrane. Cloisite-15A is natural montmorillonite modified with dimethyl dihydrogenated tallow quaternary ammonium salt. Thin-film composite (TFC) membranes were fabricated by the interfacial polymerization (IP) process between the trimesoylchloride (TMC)–n-hexane solution and m-phenylenediamine (MPD)–aqueous solution; the IP process took place on a polysulfone support sheet. The two types of nanoparticles were added in various weight ratios (0.005 wt.%–0.04 wt.%) in the n-hexane solution of TMC. Different characterizations like X-ray diffraction (XRD), contact angle, transmission electron microscopy (TEM), and membrane performance tests were performed to analyse the membrane properties. Both XRD and TEM studies proved that the two nanoclays are successfully anchored at the different sites of the PA layer. CS-15A could accelerate the water flux from 15 to 18.65 L/m2·h with NaCl rejection enhancement from 72% to 80%, relative to the control membrane. Conversely, MNT also enhanced the flux from 15 to 40 L/m2·h, but NaCl rejection reduced from 70% to 23%. The mechanism of water uptake in nanoclays was also discussed. The results pave the way for a complete future study, in which these phenomena should be studied in great detail.
Effect of Vanadium and Titanium on Desulfurization of CaO Slag in Liquid Iron
Authors: Yang Biwen , Song Bo , Li Longfei , Chen Liang
Published: November 2019

The possibility of vanadium and titanium participating in the CaO desulfurization reaction has been evaluated. The desulfurization products of CaO which were added to liquid iron containing vanadium–titanium in lab conditions were observed. At the early stage of adding CaO, titanium and sulfur ware agglomerated on the surface of CaO particles. The particles were composed of CaO, TiO2, and CaS. However, vanadium oxide was not detected. It was proposed that the titanium rather than the vanadium could react with CaO and sulfur. The desulfurization kinetics experiment showed that the high titanium content in liquid iron reduced desulfurization velocity, while vanadium had little effect on the desulfurization. The reason for this was that the TiO2 which surrounded the CaO particle impeded the mass transfer of sulfur. The vanadium and titanium had a little influence on the final sulfur content of CaO desulfurization. The theoretical discussion of these phenomena was carried out. The controlling reaction of final sulfur content was (CaO) + [S] + [C] = (CaS) + CO(g).
Grinding Kinetics of Slag and Effect of Final Particle Size on the Compressive Strength of Alkali Activated Materials
Authors: Petrakis Evangelos , Karmali Vasiliki , Bartzas Georgios , Komnitsas Konstantinos
Published: November 2019

This study aims to model grinding of a Polish ferronickel slag and evaluate the particle size distributions (PSDs) of the products obtained after different grinding times. Then, selected products were alkali activated in order to investigate the effect of particle size on the compressive strength of the produced alkali activated materials (AAMs). Other parameters affecting alkali activation, i.e., temperature, curing, and ageing time were also examined. Among the different mathematical models used to simulate the particle size distribution, Rosin–Rammler (RR) was found to be the most suitable. When piecewise regression analysis was applied to experimental data it was found that the particle size distribution of the slag products exhibits multifractal character. In addition, grinding of slag exhibits non-first-order behavior and the reduction rate of each size is time dependent. The grinding rate and consequently the grinding efficiency increases when the particle size increases, but drops sharply near zero after prolonged grinding periods. Regarding alkali activation, it is deduced that among the parameters studied, particle size (and the respective specific surface area) of the raw slag product and curing temperature have the most noticeable impact on the compressive strength of the produced AAMs.
Experimental Determination of the Effect of CaO and Al2O3 in Slag Systems Related to the Conversion Process of High Copper Matte Grade
Authors: Henao Héctor , Kohnenkamp Erik , Rojas Lisa , Moyano Alex
Published: November 2019

The slags generated in the conventional copper conversion process are mainly composed of Cu2O–Fe2O3–SiO2 with CaO, Al2O3, and MgO compounds—in concentrations up to 10 wt %. The present work contributes to the knowledge of the conversion process, generating experimental data for the phase diagrams of the Cu2O–Fe2O3–SiO2–Al2O3 and Cu2O–Fe2O3–SiO2–CaO systems. The experiments were carried out in a tubular furnace at temperatures of 1150 °C and 1200 °C, under a condition of saturation with tridymite and spinel. Once the equilibrium was reached, the samples were immediately quenched in water. The phases in the samples were observed through a scanning electron microscope (SEM) and the elemental composition of the phases were analyzed by means of energy-dispersive X-ray spectroscopy (EDS) detectors. The addition of Al2O3 and CaO into the Cu2O–Fe2O3–SiO2 system resulted in an appreciable displacement of the liquidus lines, corresponding to an expansion of the liquid in the tridymite primary phase field. The addition of CaO and Al2O3 combined was evaluated on industrial slags and from samples obtained in a Peirce–Smith furnace, with increasing amounts of CaO in the flux.
NAD Analogs in Aid of Chemical Biology and Medicinal Chemistry
Authors: Depaix Anais , Kowalska Joanna
Published: November 2019

Nicotinamide adenine dinucleotide (NAD) serves as an essential redox co-factor and mediator of multiple biological processes. Besides its well-established role in electron transfer reactions, NAD serves as a substrate for other biotransformations, which, at the molecular level, can be classified as protein post-translational modifications (protein deacylation, mono-, and polyADP-ribosylation) and formation of signaling molecules (e.g., cyclic ADP ribose). These biochemical reactions control many crucial biological processes, such as cellular signaling and recognition, DNA repair and epigenetic modifications, stress response, immune response, aging and senescence, and many others. However, the links between the biological effects and underlying molecular processes are often poorly understood. Moreover, NAD has recently been found to tag the 5′-ends of some cellular RNAs, but the function of these NAD-capped RNAs remains largely unrevealed. Synthetic NAD analogs are invaluable molecular tools to detect, monitor, structurally investigate, and modulate activity of NAD-related enzymes and biological processes in order to aid their deeper understanding. Here, we review the recent advances in the design and development of NAD analogs as probes for various cellular NAD-related enzymes, enzymatic inhibitors with anticancer or antimicrobial therapeutic potential, and other NAD-related chemical biology tools. We focus on research papers published within the last 10 years.
A Survey of Molecular Imaging of Opioid Receptors
Authors: Cumming Paul , Marton János , Lilius Tuomas O. , Olberg Dag Erlend , Rominger Axel
Published: November 2019

The discovery of endogenous peptide ligands for morphine binding sites occurred in parallel with the identification of three subclasses of opioid receptor (OR), traditionally designated as μ, δ, and κ, along with the more recently defined opioid-receptor-like (ORL1) receptor. Early efforts in opioid receptor radiochemistry focused on the structure of the prototype agonist ligand, morphine, although N-[methyl-11C]morphine, -codeine and -heroin did not show significant binding in vivo. [11C]Diprenorphine ([11C]DPN), an orvinol type, non-selective OR antagonist ligand, was among the first successful PET tracers for molecular brain imaging, but has been largely supplanted in research studies by the μ-preferring agonist [11C]carfentanil ([11C]Caf). These two tracers have the property of being displaceable by endogenous opioid peptides in living brain, thus potentially serving in a competition-binding model. Indeed, many clinical PET studies with [11C]DPN or [11C]Caf affirm the release of endogenous opioids in response to painful stimuli. Numerous other PET studies implicate μ-OR signaling in aspects of human personality and vulnerability to drug dependence, but there have been very few clinical PET studies of μORs in neurological disorders. Tracers based on naltrindole, a non-peptide antagonist of the δ-preferring endogenous opioid enkephalin, have been used in PET studies of δORs, and [11C]GR103545 is validated for studies of κORs. Structures such as [11C]NOP-1A show selective binding at ORL-1 receptors in living brain. However, there is scant documentation of δ-, κ-, or ORL1 receptors in healthy human brain or in neurological and psychiatric disorders; here, clinical PET research must catch up with recent progress in radiopharmaceutical chemistry.
A Novel Prodrug Approach for Central Nervous System-Selective Estrogen Therapy
Authors: Prokai-Tatrai Katalin , Prokai Laszlo
Published: November 2019

Beneficial effects of estrogens in the central nervous system (CNS) results from the synergistic combination of their well-orchestrated genomic and non-genomic actions, making them potential broad-spectrum neurotherapeutic agents. However, owing to unwanted peripheral hormonal burdens by any currently known non-invasive drug administrations, the development of estrogens as safe pharmacotherapeutic modalities cannot be realized until they are confined specifically and selectively to the site of action. We have developed small-molecule bioprecursor prodrugs carrying the para-quinol scaffold on the steroidal A-ring that are preferentially metabolized in the CNS to the corresponding estrogens. Here, we give an overview of our discovery of these prodrugs. Selected examples are shown to illustrate that, independently of the route of administrations and duration of treatments, these agents produce high concentration of estrogens only in the CNS without peripheral hormonal liability. 10β,17β-Dihydroxyestra-1,4-dien-3-one (DHED) has been the best-studied representative of this novel type of prodrugs for brain and retina health. Specific applications in preclinical animal models of centrally-regulated and estrogen-responsive human diseases, including neurodegeneration, menopausal symptoms, cognitive decline and depression, are discussed to demonstrate the translational potential of our prodrug approach for CNS-selective and gender-independent estrogen therapy with inherent therapeutic safety.
Temporal Trends in Maternal Food Intake Frequencies and Associations with Gestational Diabetes: The Cambridge Baby Growth Study
Authors: Petry Clive , Ong Ken , Hughes Ieuan , Acerini Carlo , Dunger David
Published: November 2019

Previous studies have suggested that in the first decade of this century the incidence of gestational diabetes (GDM) in pregnancy rose worldwide. In the Cambridge Baby Growth Study cohort we observed that this temporal trend was associated with an index of multiple deprivation and reductions in indices of insulin secretion. Deprivation level was not directly associated with GDM, suggesting that the temporal trend may relate more to other factors linked to it, such as dietary composition. In this study we investigated temporal trends in perceived food intake frequencies, derived from a qualitative, short questionnaire, in 865 pregnant Cambridge Baby Growth Study (CBGS) recruits. A number of food frequency ranks showed both temporal trends and associations with GDM, but of note is the frequency of egg consumption (negative temporal trend p = 0.03, slope = −6.2 ranks/year; negative association with GDM p = 3.0 × 10−8, slope = −0.002 increased risk/rank) as it was also positively associated with the insulin disposition index (p = 1.17 × 10−3, slope = 0.42 ranks. L/mmoL). These results are consistent with a potential protective effect of factors related to the frequency of egg consumption in pregnancy. Such factors may have contributed to the observed temporal trend in GDM risk but the overall detectable effect appears to have been small.
Dietary Trace Minerals
Authors: Tako Elad
Published: November 2019

Dietary trace minerals are pivotal and hold a key role in numerous metabolic processes. Trace mineral deficiencies (except for iodine, iron, and zinc) do not often develop spontaneously in adults on ordinary diets; infants are more vulnerable because their growth is rapid and intake varies. Trace mineral imbalances can result from hereditary disorders (e.g., hemochromatosis, Wilson disease), kidney dialysis, parenteral nutrition, restrictive diets prescribed for people with inborn errors of metabolism, or various popular diet plans. The Special Issue “Dietary Trace Minerals” comprised 13 peer-reviewed papers on the most recent evidence regarding the dietary intake of trace minerals, as well as their effect toward the prevention and treatment of non-communicable diseases. Original contributions and literature reviews further demonstrated the crucial and central part that dietary trace minerals play in human health and development. This editorial provides a brief and concise overview that addresses and summarizes the content of the Dietary Trace Minerals Special Issue.
Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium sidoides DC Root Extract
Authors: Jekabsone Aiste , Sile Inga , Cochis Andrea , Makrecka-Kuka Marina , Laucaityte Goda , Makarova Elina , Rimondini Lia , Bernotiene Rasa , Raudone Lina , Vedlugaite Evelina , Baniene Rasa , Smalinskiene Alina , Savickiene Nijole , Dambrova Maija
Published: November 2019

The study explores antibacterial, antiinflammatory and cytoprotective capacity of Pelargonium sidoides DC root extract (PSRE) and proanthocyanidin fraction from PSRE (PACN) under conditions characteristic for periodontal disease. Following previous finding that PACN exerts stronger suppression of Porphyromonas gingivalis compared to the effect on commensal Streptococcus salivarius, the current work continues antibacterial investigation on Staphylococcus aureus, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans and Escherichia coli. PSRE and PACN are also studied for their ability to prevent gingival fibroblast cell death in the presence of bacteria or bacterial lipopolysaccharide (LPS), to block LPS- or LPS + IFNγ-induced release of inflammatory mediators, gene expression and surface antigen presentation. Both PSRE and PACN were more efficient in suppressing Staphylococcus and Aggregatibacter compared to Escherichia, prevented A. actinomycetemcomitans- and LPS-induced death of fibroblasts, decreased LPS-induced release of interleukin-8 and prostaglandin E2 from fibroblasts and IL-6 from leukocytes, blocked expression of IL-1β, iNOS, and surface presentation of CD80 and CD86 in LPS + IFNγ-treated macrophages, and IL-1β and COX-2 expression in LPS-treated leukocytes. None of the investigated substances affected either the level of secretion or expression of TNFα. In conclusion, PSRE, and especially PACN, possess strong antibacterial, antiinflammatory and gingival tissue protecting properties under periodontitis-mimicking conditions and are suggestable candidates for treatment of the disease.
The Fluid Aspect of the Mediterranean Diet in the Prevention and Management of Cardiovascular Disease and Diabetes: The Role of Polyphenol Content in Moderate Consumption of Wine and Olive Oil
Authors: Ditano-Vázquez Paola , Torres-Peña José David , Galeano-Valle Francisco , Pérez-Caballero Ana Isabel , Demelo-Rodríguez Pablo , Lopez-Miranda José , Katsiki Niki , Delgado-Lista Javier , Alvarez-Sala-Walther Luis A.
Published: November 2019

A growing interest has emerged in the beneficial effects of plant-based diets for the prevention of cardiovascular disease, diabetes and obesity. The Mediterranean diet, one of the most widely evaluated dietary patterns in scientific literature, includes in its nutrients two fluid foods: olive oil, as the main source of fats, and a low-to-moderate consumption of wine, mainly red, particularly during meals. Current mechanisms underlying the beneficial effects of the Mediterranean diet include a reduction in inflammatory and oxidative stress markers, improvement in lipid profile, insulin sensitivity and endothelial function, as well as antithrombotic properties. Most of these effects are attributable to bioactive ingredients including polyphenols, mono- and poly-unsaturated fatty acids. Polyphenols are a heterogeneous group of phytochemicals containing phenol rings. The principal classes of red wine polyphenols include flavonols (quercetin and myricetin), flavanols (catechin and epicatechin), anthocyanin and stilbenes (resveratrol). Olive oil has at least 30 phenolic compounds. Among them, the main are simple phenols (tyrosol and hydroxytyrosol), secoroids and lignans. The present narrative review focuses on phenols, part of red wine and virgin olive oil, discussing the evidence of their effects on lipids, blood pressure, atheromatous plaque and glucose metabolism.
Atorvastatin and Vitamin E Accelerates NASH Resolution by Dietary Intervention in a Preclinical Guinea Pig Model
Authors: Klaebel Julie Hviid , Skjødt Mia , Skat-Rørdam Josephine , Rakipovski Günaj , Ipsen David H. , Schou-Pedersen Anne Marie V. , Lykkesfeldt Jens , Tveden-Nyborg Pernille
Published: November 2019

Despite affecting millions of patients worldwide, no pharmacological treatment has yet proved effective against non-alcoholic steatohepatitis (NASH) induced liver fibrosis. Current guidelines recommend lifestyle modifications including reductions in dietary energy intake. Recently, therapy with atorvastatin and vitamin E (vitE) has been recommended, although clinical studies on the resolution of hepatic fibrosis are inconclusive. Targeting NASH-induced hepatic end-points, this study evaluated the effects of atorvastatin and vitE alone or in combination with a dietary intervention in the guinea pig NASH model. Guinea pigs (n = 72) received 20 weeks of high fat feeding before allocating to four groups: continued HF feeding (HF), HF diet with atorvastatin and vitE (HF+), low-fat diet (LF) and low-fat with atorvastatin and vitE (LF+), for four or eight weeks of intervention. Both LF and LF+ decreased liver weight, cholesterol and plasma dyslipidemia. LF+ further improved hepatic histopathological hallmarks (p < 0.05), liver injury markers aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (p < 0.05) and reduced the expression of target genes of hepatic inflammation and fibrosis (p < 0.05), underlining an increased effect on NASH resolution in this group. Collectively, the data support an overall beneficial effect of diet change, and indicate that atorvastatin and vitE therapy combined with a diet change act synergistically in improving NASH-induced endpoints.
Effect of Lactobacillus plantarum TWK10 on Exercise Physiological Adaptation, Performance, and Body Composition in Healthy Humans
Authors: Huang Wen-Ching , Lee Mon-Chien , Lee Chia-Chia , Ng Ker-Sin , Hsu Yi-Ju , Tsai Tsung-Yu , Young San-Land , Lin Jin-Seng , Huang Chi-Chang
Published: November 2019

Probiotics have been rapidly developed for health promotion, but clinical validation of the effects on exercise physiology has been limited. In a previous study, Lactobacillus plantarum TWK10 (TWK10), isolated from Taiwanese pickled cabbage as a probiotic, was demonstrated to improve exercise performance in an animal model. Thus, in the current study, we attempted to further validate the physiological function and benefits through clinical trials for the purpose of translational research. The study was designed as a double-blind placebo-controlled experiment. A total of 54 healthy participants (27 men and 27 women) aged 20–30 years without professional athletic training were enrolled and randomly allocated to the placebo, low (3 × 1010 colony forming units (CFU)), and high dose (9 × 1010 CFU) TWK10 administration groups (n = 18 per group, with equal sexes). The functional and physiological assessments were conducted by exhaustive treadmill exercise measurements (85% VO2max), and related biochemical indices were measured before and after six weeks of administration. Fatigue-associated indices, including lactic acid, blood ammonia, blood glucose, and creatinine kinase, were continuously monitored during 30 min of exercise and a 90 min rest period using fixed intensity exercise challenges (60% VO2max) to understand the physiological adaptation. The systemic inflammation and body compositions were also acquired and analyzed during the experimental process. The results showed that TWK10 significantly elevated the exercise performance in a dose-dependent manner and improved the fatigue-associated features correlated with better physiological adaptation. The change in body composition shifted in the healthy direction for TWK10 administration groups, especially for the high TWK10 dose group, which showed that body fat significantly decreased and muscle mass significantly increased. Taken together, our results suggest that TWK10 has the potential to be an ergogenic aid to improve aerobic endurance performance via physiological adaptation effects.
Characterization and Pathogenicity of Fusarium Species Associated with Soybean Pods in Maize/Soybean Strip Intercropping
Authors: Naeem Muhammd , Li Hongju , Yan Li , Raza Muhammad Ali , Gong Guoshu , Chen Huabao , Yang Chunping , Zhang Min , Shang Jing , Liu Taiguo , Chen Wanquan , Fahim Abbas Muhammad , Irshad Gulshan , Ibrahim Khaskheli Muhammad , Yang Wenyu , Chang Xiaoli
Published: November 2019

Intercropping has been considered as a kind of a sustainable agricultural cropping system. In southwest China, maize/soybean strip intercropping has commonly been practised under local limited agricultural land resources. However, heavy rainfall in combination with high humidity and low temperatures cause severe pod and seed deterioration in the maturity and pre-harvesting stages of intercropped soybean. Numerous Fusarium species have been reported as the dominant pathogens of soybean root rot, seedling blight, as well as pod field mold in this area. However, the diversity and pathogenicity of Fusarium species on soybean pods remain unclear. In the current study, diseased soybean pods were collected during the cropping season of 2018 from five different intercropped soybean producing areas. A total of 83 Fusarium isolates were isolated and identified as F. fujikuroi, F. graminearum, F. proliferatum, and F. incarnatum-equiseti species complex based on morphological characteristics and phylogenetic analysis of the nucleotide sequence of EF1-α and RPB2 genes. Pathogenicity tests demonstrated that all Fusarium species were pathogenic to seeds of the intercropped soybean cultivar Nandou12. Fusarium fujikuroi had the maximum disease severity, with a significant reduction of seed germination rate, root length, and seed weight, followed by F. equiseti, F. graminearum, F. proliferatum, and F. incarnatum. Additionally, the diversity of Fusarium species on soybean pods was also considerably distinct according to the geographical origin and soybean varieties. Thus, the findings of the current study will be helpful for the management and resistance breeding of soybean pod decay in the maize/soybean intercropping system.
Biological Effects of Chitosan-Based Dressing on Hemostasis Mechanism
Authors: Wang Yi-Wen , Liu Chuan-Chieh , Cherng Juin-Hong , Lin Chien-Seng , Chang Shu-Jen , Hong Zhi-Jie , Liu Cheng-Che , Chiu Yaw-Kwan , Hsu Sheng-Der , Chang Hung
Published: November 2019

There have been numerous recent advances in wound care management. Nevertheless, the assessment of hemostatic dressing is essential to enable surgeons and other physicians and healthcare professionals to make the correct decisions regarding the disposition of severe hemorrhage. Here, we investigated the relative efficacies of chitosan-based and conventional gauze dressings in a rat model of femoral artery hemorrhage and in patients with surgical wounds. Dressing effectiveness was evaluated based on hemostatic profiles, biocompatibility, antimicrobial activity, and blood factor responses in coagulation. Relative to standard gauze dressing, the chitosan fiber (CF) dressing treatment significantly shortened the time to hemostasis in injured rats. Moreover, the CF dressing significantly prolonged partial thromboplastin time, enhanced blood absorption, and reduced antithrombin production without altering the prothrombin ratio. Unlike regular gauze bandages, the CF dressing demonstrated remarkable antibacterial activity. The results of this study indicate the effectiveness of chitosan as a hemostatic dressing and elucidate its underlying mechanism. It is possible that chitosan surgical dressings could serve as first-line intervention in hospital emergency care for uncontrolled hemorrhage.
A Review of First-Passage Theory for the Segerdahl-Tichy Risk Process and Open Problems
Authors: Avram Florin , Perez-Garmendia Jose-Luis
Published: November 2019

The Segerdahl-Tichy Process, characterized by exponential claims and state dependent drift, has drawn a considerable amount of interest, due to its economic interest (it is the simplest risk process which takes into account the effect of interest rates). It is also the simplest non-Lévy, non-diffusion example of a spectrally negative Markov risk model. Note that for both spectrally negative Lévy and diffusion processes, first passage theories which are based on identifying two “basic” monotone harmonic functions/martingales have been developed. This means that for these processes many control problems involving dividends, capital injections, etc., may be solved explicitly once the two basic functions have been obtained. Furthermore, extensions to general spectrally negative Markov processes are possible; unfortunately, methods for computing the basic functions are still lacking outside the Lévy and diffusion classes. This divergence between theoretical and numerical is strikingly illustrated by the Segerdahl process, for which there exist today six theoretical approaches, but for which almost nothing has been computed, with the exception of the ruin probability. Below, we review four of these methods, with the purpose of drawing attention to connections between them, to underline open problems, and to stimulate further work.
Analysis of the Optimal Wavelength for Oceanographic Lidar at the Global Scale Based on the Inherent Optical Properties of Water
Authors: Chen Shuguo , Xue Cheng , Zhang Tinglu , Hu Lianbo , Chen Ge , Tang Junwu
Published: November 2019

Understanding the optimal wavelength for detecting the water column profile from a light detection and ranging (lidar) system is important in the design of oceanographic lidar systems. In this research, the optimal wavelength for detecting the water column profile using a lidar system at the global scale was analyzed based on the inherent optical properties of water. In addition, assuming that the lidar system had a premium detection characteristic in its hardware design, the maximum detectable depth using the established optimal wavelength was analyzed and compared with the mixed layer depth measured by Argo data at the global scale. The conclusions drawn are as follows: first, the optimal wavelengths for the lidar system are between the blue and green bands. For the open ocean, the optimal wavelengths are between 420 and 510 nm, and for coastal waters, the optimal wavelengths are between 520 and 580 nm. To obtain the best detection ability using a lidar system, the best configuration is to use a lidar system with multiple bands. In addition, a 490 nm wavelength is recommended when an oceanographic lidar system is used at the global scale with a single wavelength. Second, for the recommended 490 nm band, a lidar system with the 4 attenuating length detection ability can penetrate the mixed layer for 80% of global waters.
Assessment of Portable Chlorophyll Meters for Measuring Crop Leaf Chlorophyll Concentration
Authors: Dong Taifeng , Shang Jiali , Chen Jing M. , Liu Jiangui , Qian Budong , Ma Baoluo , Morrison Malcolm J. , Zhang Chao , Liu Yupeng , Shi Yichao , Pan Hui , Zhou Guisheng
Published: November 2019

Accurate measurement of leaf chlorophyll concentration (LChl) in the field using a portable chlorophyll meter (PCM) is crucial to support methodology development for mapping the spatiotemporal variability of crop nitrogen status using remote sensing. Several PCMs have been developed to measure LChl instantaneously and non-destructively in the field, however, their readings are relative quantities that need to be converted into actual LChl values using conversion functions. The aim of this study was to investigate the relationship between actual LChl and PCM readings obtained by three PCMs: SPAD-502, CCM-200, and Dualex-4. Field experiments were conducted in 2016 on four crops: corn (Zea mays L.), soybean (Glycine max L. Merr.), spring wheat (Triticum aestivum L.), and canola (Brassica napus L.), at the Central Experimental Farm of Agriculture and Agri-Food Canada in Ottawa, Ontario, Canada. To evaluate the impact of other factors (leaf internal structure, leaf pigments other than chlorophyll, and the heterogeneity of LChl distribution) on the conversion function, a global sensitivity analysis was conducted using the PROSPECT-D model to simulate PCM readings under different conditions. Results showed that Dualex-4 had a better performance for actual LChl measurement than SPAD-502 and CCM-200, using a general conversion function for all four crops tested. For SPAD-502 and CCM-200, the error in the readings increases with increasing LChl. The sensitivity analysis reveals that deviations from the calibration functions are more induced by non-uniform LChl distribution than leaf architectures. The readings of Dualex-4 can have a better ability to restrict these influences than those of the other two PCMs.
Auction-Based Secondary Relay Selection on Overlay Spectrum Sharing in Hybrid Satellite–Terrestrial Sensor Networks
Authors: Zhang Xiaokai , Zhang Bangning , An Kang , Chen Zhuyun , Guo Daoxing
Published: November 2019

In this paper, we investigate the auction-based secondary relay selection on overlay spectrum sharing in hybrid satellite–terrestrial sensor networks (HSTSNs), where both the decode-and-forward (DF) and amplify-and-forward (AF) relay protocols are analyzed based on time division multiple access (TDMA). As both the primary and secondary networks are rational, honest but with incomplete network information, they prefer to obtain maximum possibility payoffs by the cooperation between the primary and secondary networks, and the competition among secondary networks. Hence, Vickery auction is introduced to achieve the effective and efficient secondary relay selection by distinct sub-time slot allocation for one shot in terms of a distributed manner. Finally, numerical simulations are provided to validate the effectiveness of the auction mechanism on cooperative spectrum sharing in HSTSNs for secondary relay selection. Besides, the effect of key factors on the performance of the auction mechanism are analyzed in details.
Technology Support for Collaborative Preparation of Emergency Plans
Authors: Baloian Nelson , Frez Jonathan , Pino Jose A. , Peñafiel Sergio , Zurita Gustavo , Abarca Alvaro
Published: November 2019

Preparing a plan for reaction to a grave emergency is a significant first stage in disaster management. A group of experts can do such preparation. Best results are obtained with group members having diverse backgrounds and access to different relevant data. The output of this stage should be a plan as comprehensive as possible, taking into account various perspectives. The group can organize itself as a collaborative decision-making team with a process cycle involving modeling the process, defining the objectives of the decision outcome, gathering data, generating options and evaluating them according to the defined objectives. The meeting participants may have their own evidences concerning people’s location at the beginning of the emergency and assumptions about people’s reactions once it occurs. Geographical information is typically crucial for the plan, because the plan must be based on the location of the safe areas, the distances to move people, the connecting roads or other evacuation links, the ease of movement of the rescue personnel, and other geography-based considerations. The paper deals with this scenario and it introduces a computer tool intended to support the experts to prepare the plan by incorporating the various viewpoints and data. The group participants should be able to generate, visualize and compare the outcomes of their contributions. The proposal is complemented with an example of use: it is a real case simulation in the event of a tsunami following an earthquake at a certain urban location.
Multifunctional Medical Recovery and Monitoring System for the Human Lower Limbs
Authors: Comanescu Adriana , Dugaesescu Ileana , Boblea Doru , Ungureanu Liviu
Published: November 2019

In order to develop multifunctional medical recovery and monitoring equipment for the human lower limb, a new original mechanical structure with three degrees mobility has been created for the leg sagittal model. This mechanism is integrated in the equipment and includes elements that have similar functions to the different anatomic parts (femur, median part), leg, and foot. The independent relative rotation motion between the previously mentioned anatomic parts is ensured. The femur may have an oscillation rotation of about 100° relative to the trunk. The median part (leg) alternatively rotates 150° relative to the superior segment. The lower part (foot) is initially placed at 90° relative to the median part and may have an alternative rotation of 25°. Depending on a patient’s medical needs and their recovery progress, device sensors provide varying angular amplitude of different segments of the human limb. Moreover, the mechanism may actuate either anatomic leg segment, two parts, or all of them.