bluuebunny/crossref_metadata_2025 huggingface dataset:
Hubungan Hiperglikemia Reaktif pada Stroke Iskemik Fase Akut dengan Gangguan Motorik Terhadap Keluaran Pasien Stroke Berdasarkan Indeks Barthel di Rumah Sakit Siti Khodijah Sepanjang
Authors: Savitri Delfia , Irfana Laily , Irawati Detti Nur , Prahasanti Kartika
Published: December 2020

Pendahuluan: Menurut data WHO tahun 2012, stroke merupakan penyakit yang menyebabkan kematian terbesar ketiga di seluruh dunia setelah kanker dan penyakit jantung koroner serta terdapat 6,2 juta kematian disebabkan oleh stroke. Pada kondisi stroke akut sering kali mengalami hipertensi, hiperglikemia,dan leukositosis. Kenaikan  kadar gula darah  pada pasien stroke yang tidak memiliki riwayat diabetes sebelumnya disebut dengan hiperglikemia reaktif. Hal ini dapat mempengaruhi mortalitas dan morbiditas dari penderita stroke. Dimana kelainan ini kemungkinan disebabkan oleh peningkatan drastis sekresi kortisol sebagai respon terhadap segala jenis situasi stress yang diperantarai oleh susunan sistem saraf pusat melalui peningkatan aktivitas sistem aksis hipotalamus-pituitari-adrenal. penelitian ini dilakukan untuk melihat outcome atau hasil keluaran dari pasien stroke akut yang mengalami hiperglikemia dan akan dievaluasi dengan Indeks Barthel.Tujuan: Untuk mengetahui adanya hubungan antara hiperglikemia reaktif pada stroke fase akut dengan keluaran pasien stroke berdasarkan Indeks Barthel di Rumah Sakit Siti Khodijah SepanjangMetode: Menggunakan desain cross sectional dengan teknik pengambilan sampel sampling from consecutive admission yaitu menurut kasus yang datang berturut-turut sampai jumlah sampel terpenuhi. Penelitian ini dimulai dengan mengambil daftar pasien yang mengalami stroke iskemik di Instalasi Gawat Darurat, kemudian mengambil data pasien di ruangan rawat inap dengan melihat berkas rekam medis pasien untuk melihat nilai gula darah acak pasien pada fase akut serangan stroke, setelah itu melakukan penilaian Indeks Barthel pada hari kelima pasien dirawat.Hasil: Hasil uji statistik menggunakan Koefisien Kontingensi didapatkan nilai signifikan p: 0.039 (p<0,05)Kesimpulan:Hasil penelitian ini menunjukkan adanya hubungan antara hiperglikemia reaktif pada stroke fase akut dengan keluaran pasien stroke berdasarkan Indeks Barthel di Rumah Sakit Siti Khodijah Sepanjang
Hubungan Luas Ventilasi dan Pencahayaan Alami Rumah terhadap Tingkat Kepositifan Sputum BTA pada Penderita TB Paru di Puskesmas Tlogosadang
Authors: Dinata M. Thoriq Satria , Subkhan Muhammad , Ghufron Musa
Published: December 2020

Latar Belakang: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Mycobacterium tuberculosis. Indonesia di peringkat ketiga di dunia menyumbang 8% untuk kasus TB. Fenomena yang terjadi di wilayah kerja Puskesmas Tlogosadang yaitu adanya peningkatan kasus TB BTA positif. Hal ini diduga disebabkan oleh faktor kondisi fisik rumah pasien TB paru khususnya luas ventilasi dan pencahayaan alaminya yang tidak terstandar.Tujuan: Menganalisis hubungan luas ventilasi dan pencahayaan alami rumah terhadap tingkat kepositifan sputum BTA pada penderita TB paru di Puskesmas Tlogosadang.Metode: Penelitian ini menggunakan desain kohort retrospektif. Responden penelitian diambil dengan metode simple random sampling pada 38 penderita TB paru BTA positif pada tahun 2016 - 2018. Pengamatan luas ventilasi (minimal 10% dari luas lantai), sedangkan pencahayaan alami (minimal 60 lux). Pengamatan rekam medis tingkat kepositifan BTA (Scanty, +1, +2, dan +3) dilakukan di Laboratorium Puskesmas Tlogosadang, kemudian dicatat dalam check list observasi.Hasil: Didapatkan 3 responden dengan BTA scanty, 20 responden (+1), 14 responden (+2), dan 1 responden (+3). Lebih dari 65 % luas ventilasi dan pencahayaan alami rumah tidak terstandar. Hasil analisis uji koefisien kontingensi menunjukkan hubungan luas ventilasi (p = 0,60) dan pencahayaan alami (p = 0,24) terhadap tingkat kepositifan sputum BTA.Kesimpulan: Tidak terdapat hubungan luas ventilasi dan pencahayaan alami rumah terhadap tingkat kepositifan sputum BTA pada penderita TB paru di Puskesmas Tlogosadang.
Influence of Income and Knowledge about Mosquito Nest Eradication (PSN DBD) to The Presence of Aedes aegypti Larvae
Authors: Bestari Rochmadina Suci
Published: December 2020

DHF still become health problem in Indonesia. In Central Java, especially Sukoharjo district, Angka Bebas Jentik (ABJ) is still under national standard. Some factors that influence the existence of larvae are the level of income and knowledge about PSN. Consumerism triggered escalation amount of  mosquito breeding sites due to ownership of non-degradable goods like plastic containers, paper cups, and so on. Consumerism is related to income. This study has purpose to determine corelation between income levels and knowledge about PSN with existence of Aedes aegypti larvae in Karangtengah Village. Designed with cross-sectional approach. The population were residences that has Family Card in Karangtengah Village with a total of 1660 households taken 65 households. The independent variable is the level of income and knowledge about PSN. Dependent variable is existence of larvae. Data analysis used Chi-Square test and contingency coefficient (C). The results showed income with the existence of larvae (container index) (p = 0.009, C = 0.308) and PSN knowledge in the presence of larvae (container index) (p = 0.005, C = 0.332). The results of data analysis show that there is a correlation between income and knowledge about PSN with the existence of larvae. This study showed there is a relationship between income and knowledge about PSN with the existence of larvae.
PENGARUH POLA MAKAN DAN AKTIVITAS FISIK TERHADAP PENURUNAN KADAR GULA DARAH PADA PASIEN DIABETES MELITUS TIPE 2 DI KLINIK AULIA JOMBANG
Authors: Himmah Siti Cholishotul , Irawati Detty Nur , Triastuti Nenny , Salim Nabil
Published: December 2020

Latar Belakang : Diabetes melitus di Indonesia merupakan salah satu penyakit tidak menular dengan kematian tertinggi. Diabetes melitus tipe 2 ditandai dengan kerja insulin yang tidak efektif, sehingga tidak berfungsi optimal dalam mengatur metabolisme glukosa. Penyakit ini dapat disebabkan dari beberapa faktor salah satunya adalah gaya hidup yang tidak sehat, diantaranya latihan fisik dan pola makan yang tidak baik. Tujuan :  Untuk mengetahui pengaruh pola makan dan aktivitas fisik pada penurunan kadar gula darah pada pasien diabetes melitus tipe 2. Metode : Penelitian ini menggunakan rancangan analitik Cross sectional. Populasi dalam penelitian ini adalah seluruh peserta yang mengikuti Prolanis di Klinik Aulia Jombang. Teknik pengambilan sampel dalam penelitian ini adalah purposive sampling. Sampel pada penelitian ini adalah peserta yang mengikuti Prolanis di Klinik Aulia Jombang, yang juga merupakan pasien diabetes melitus tipe 2. Instrumen yang digunakan adalah data primer berupa kuesioner dan data sekunder berupa rekam medis pasien. Hasil : Hasil analisis dengan mann-whitney test menunjukkan signifikansi pengaruh pola makan dan aktivitas fisik terhadap penurunan kadar gula darah (p kurang dari 0,05). Kesimpulan : Menunjukkan bahwa terdapat pengaruh yang signifikan polaimakan dan aktivitas fisik terhadap penurunan kadar gula darah
Pola Sidik Jari dan Besaran Sudut Axial Triradius Digital (ATD) pada Pengguna Narkotika Jenis Shabu-Shabu di Kota Palembang
Authors: Mundijo Trisnawati
Published: December 2020

Dermatoglyphics is a science to determine a pattern both from fingerprints, triradius, tendrils, and the Axial Triradius Digital (ATD) angle. Nowadays, it is can be used in ethnhobiology, forensic or to determine the pattern the disease or disorder. Research on dermatoglyphics is still needs study. Today, research on fingerprint patterns, especially in drug cases is still very limited, so that research is conducted to find out what is the dominance of the fingerprint pattern and ATD angle in people with drug cases. The research was conducted in the National Narcotics Agency (BNN) of South Sumatra and the Cahaya Putra Rehabilitation Foundation in Palembang, South Sumatra. Samples were carried out by total sampling from the two places was 30 peoples, all of whom were drug users of the type of methamphetamine. Fingerprint pattern data were collected using the U.Are.U Digital Personal tool. Fingerprint reader attaches the fingerprints of the ten fingers, while the ATD angle calculation is done by smearing lipstick on both palms of the sample and pasting them on the observation sheet. The data obtained were analyzed, and the results for the fingerprint patterns were at most with a whorl pattern of 99 fingers (41.25%). Whereas for the ATD angle, the highest results were obtained at angles> 50o from both hands as many as 14 people (58.3%). The conclusion of this study is the most dermatoglyphic characteristics of drug users are whorl patterns with an ATD angle> 50o.
Penerapan Terapi Pijat Abdoment Pada Lanjut Usia
Authors: Munira Munira , Aisah Siti
Published: December 2020

Penurunan struktur dan fungsi pada sistem gastrointensinal lansia dapat menyebabkan konstipasi, hal ini karena waktu pengosongan lambung menjadi lebih lama, peristaltik usus melemah dan kemampuan absorbsi menurun.  Konstipasi harus segera ditangani karena akan berdampak robeknya kulit pada dinding anus yang menyebabkan buang air besar berdarah. Tujuan studi kasus ini adalah memberikan asuhan keperawatan dengan intervensi massage abdomen dalam penurunan tingkat konstipasi pada usia lanjut. Metode penulisan ini menggunakan desain studi kasus dengan pendekatan proses keperawatan yang meliputi pengkajian, analisa data, intervensi keperawatan, implementasi dan evaluasi. Populasi dalam studi kasus ini adalah semua lansia  di ruang Cempaka Rumah Pelayanan Sosial Lanjut Usia Pucanggading Semarang yang berjumlah 38 responden. Jumlah sampel yang digunakan dalam studi kasus ini sebanyak 2 responden yang diambil menggunakan teknik Purposive sampling. Penerapan dilakukan selama 7 hari dengan pemberian tindakan keperawatan berupa terapi massage abdomen dengan frekuensi 1 kali sehari selama 15 menit dipagi hari. Pengumpulan data menggunakan teknik wawancara. Kriteria hasil menggunakan Constipation Scoring System (CSS). Setelah dilakukan massage abdomen selama 7 hari, terjadi penurunkan konstipasi pada lansia yang di buktikan dari hasil pengukuran menggunakan Constipation Scoring System (CSS). Kedua Pasien mengalami peningkatan frekuensi defekasi, mengedan saat defekasi menurun, merasa tuntas setelah defekasi, perasaan tidak nyaman pada perut menjadi hilang. Kesimpulannya adalah teknik massage abdomen dapat menurunkan tingkat konstipasi.
Penurunan Glukosa Darah Pasien Diabetes Melitus Tipe 2 Menggunakan Kombinasi Terapi Relaksasi Napas Dalam dan Murrotal
Authors: Setiawan Muhammad Duwi , Yanto Arief
Published: December 2020

Dampak hiperglikemia pasien Diabetes Melitus (DM) tipe 2 menyebabkan terjadinya resiko infeksi, gagal jantung, sroke, dan hipertensi. Kombinasi terapi napas dalam dan murrrotal Ar-Rahman mampu menekan hormon stres dan meningkatkan hormon endorphin kemudian menghambat konversi glikogen yang tersimpan di hati menjadi glukosa sehingga menurunkan glukosa darah sewaktu (GDS). Studi kasus ini bertujuan untuk mengetahui penurunan GDS pada pasien DM tipe 2 setelah dilakukan kombinasi terapi napas dalam dan murrrotal Ar-Rahman. Desain studi kasus ini menggunakan deskriptif dengan pendekatan proses asuhan keperawatan. Subjek studi kasus adalah pasien DM tipe 2 yang menjalani rawat inap. Subjek studi kasus berjumlah 2 pasien, yang didapatkan secara purposive dan random sampling. Pengambilan data menggunakan instrumen mp3 murrotal, aerphone, glucometer. Pegukuran GDS sebelum dan sesudah dilakukan kombinasi terapi napas dalam dan murrotal Ar-Rahman selama 20 menit sebelum pemberian terapi obat DM. Pasien telah menandatangi lembar persetujuan. Hasil studi kasus menunjukan rata-rata GDS kedua subjek studi kasus mengalami penurunan setelah diberikan kombinasi relaksasi napas dalam dan terapi murrotal Ar-Rahman sebesar 7,45%. Kombinasi terapi napas dalam dan murrrotal Ar-Rahman mampu menurunan GDS pada pasien DM tipe 2. Diharapkan perawat mampu menerapkan kombinasi terapi napas dalam dan murrrotal Ar-Rahman pada pasien DM tipe 2 untuk menurunkan GDS.
Kombinasi Kompres Hangat Dengan Teknik Blok Dan Teknik Seka (Tepid Sponge Bath) Menurunkan Suhu Tubuh Pada Anak Penderita Gastroentritis
Authors: Irmachatshalihah Rastia , Alfiyanti Dera
Published: December 2020

Gastroenteritis merupakan jenis infeksi saluran pencarnaan yang memiliki insiden paling tinggi pada anak. Manifestasi klinis gastroenteritis yaitu diare, muntah, dehidrasi dan hipertermia. Manajemen hipertermia sangat penting untuk menurunkan suhu tubuh, karena hipertermia pada kasus ini berhubungan dengan proses infeksi dan dehidrasi. Peningkatan suhu tubuh yang terlalu tinggi dapat memicu dehidrasi, letargi, dan kejang. Manajemen hipertermi non farmakologi yang efektif sesuai hasil penelitian adalah kombinasi kompres hangat dengan teknik blok dan teknik seka (tepid sponge bath). Kombinasi dua teknik kompres ini meningkatkan kontrol kehilangan panas secara evaporasi dan konveksi. Studi kasus ini bertujuan untuk mengetahui kombinasi kompres hangat teknik blok dan teknik seka (tepid sponge bath) dalam menurunkan suhu tubuh. Studi kasus ini menggunakan desain deskriptif dengan pendekatan asuhan keperawatan. Subjek studi kasus adalah dua pasien anak gastroenteritis yang mengalami demam. Hasil studi kasus ini menunjukan setelah dilakukan kombinasi kompres hangat dengan teknik blok dan teknik seka suhu tubuh mengalami penurunan. Rerata penurunan suhu tubuh pada responden pertama dan kedua adalah 0,5oC. Kombinasi kompres hangat dengan teknik blok dan teknik seka (tepid sponge bath) dapat menurunkan suhu tubuh pada pasien gastroentritis yang mengalami hipertermia.
Custom Practices of English Education at the Rural Primary Schools in Bangladesh
Authors: Khan Mohammad Ehsanul Islam , Siddique Md. Abu Bakar , Shiblu Mohammad Nazmul Haque Shikder
Published: December 2020

This comprehensive study dealt with the existing traditional practices of English education at rural primary schools in Bangladesh. The researchers collected data from twenty-five assistant teachers of thirteen primary schools located in the rural areas of four different districts of Dhaka and Mymensingh divisions. These English teaching participants also represent the key informants for related queries. The study used a semi-structured questionnaire with ten comprehensive and open-ended questions related to the existing teaching patterns with face-to-face interviews on the collected responses of the participants. All the data were interpreted in a qualitative approach. The study revealed the educational constrictions of the current ‘English as a Foreign Language (EFL)’ situation emphasizing the implementation of practical activities as a solution for the kids and suggested some development aspects from teachers’ perspectives. The research explored the flaws of current teaching strategies and materials used in primary EFL education and recommended some practical tactics to include in the curriculum for the development of primary EFL education in Bangladesh.
Advancing the Design of Self-Explanation Prompts for Complex Problem-Solving
Authors: Joo Hyun , Lee Jinju , Kim Dongsik
Published: December 2020

This research investigated the effects of focus (inference vs. inference followed by integration) and level (low vs. middle vs. high) in self-explanation prompts on both cognitive load and learning outcomes. To achieve this goal, a 2*3 experiment design was employed. A total of 199 South Korean high school students were randomly assigned to one of six conditions. The two-way MANOVA was used to analyse the effects of the self-explanation prompts on learning outcomes. Results showed that there was an interaction effect between focus and level of self-explanation prompts on delayed conceptual knowledge, suggesting that the focus of self-explanation prompts could be varied depending on their level. Second, learners who were given a high level of prompts scored higher on the immediate conceptual knowledge test than those who received a low level of prompts. A two-way ANOVA was conducted to analyse the effects of the self-explanation prompts on cognitive load and showed no significant interaction effect. However, there was a main effect in the level of the prompt that a high level of self-explanation prompts imposed a lower cognitive load compared to a low level of prompts. In sum, the design and development of self-explanation prompts should consider both focus and level, especially to improve complex problem-solving skills.
PENGARUH PMA, PMDN, TPAK, PDRB PERKAPITA, PENGELUARAN PEMERINTAH TERHADAP DISPARITAS PENDAPATAN JAWA
Authors: Laut Lorentino Togar , Putri Arinda Sita , Septiani Yustirania
Published: December 2020

AbstrakDistribusi pendapatan di Pulau Jawa masih belum merata, pertumbuhan ekonomi di Pulau Jawa belum mampu mencapai kesejahteraan ekonomi yang merata. Sehingga penelitian ini bertujuan untuk mengetahui bagaimana pengaruh penanaman modal asing, penanaman modal dalam negeri, tingkat partisipasi angkatan kerja, PDRB perkapita, dan pengeluaran pemerintah terhadap disparitas pendapatan di Pulau Jawa tahun 2008-2018. Jenis data adalah data sekunder yang bersumber dari Badan Pusat Statistik Indonesia dan Badan Koordinasi Penanaman Modal. Analisis data menggunakan regresi data panel Eviews 7. Hasil penelitian menunjukkan bahwa penanaman modal asing, penanaman modal dalam negeri dan pengeluaran pemerintah tidak berpengaruh signifikan terhadap disparitas pendapatan di Pulau Jawa tahun 2008-2018. Sedangkan variabel tingkat partisipasi angkatan kerja, PDRB perkapita, berpengaruh signifikan terhadap disparitas pendapatan di Pulau Jawa tahun 2008-2018. Dari seluruh variabel berpengaruh secara simultan terhadap disparitas di Pulau Jawa tahun 2008-2018.Kata Kunci :  Disparitas pendapatan; Kesejahteraan Ekonomi; Pertumbuhan Ekonomi;AbstractThe distribution of income in Java Island is still uneven, economic growth in Java has not been able to achieve equitable economic prosperity. So this study aims to determine how the effect of foreign investment, domestic investment, labor force participation rate, GDP per capita, and government spending on income disparities in Java in 2008-2018. The type of data is secondary  data sourced from the Indonesian Central Bureau of Statistics and the Investment Coordinating Board. Data analysis used Eviews 7 panel data regression. The results showed that foreign investment, domestic investment and government spending did not have a significant effect on income disparities in Java in 2008-2018. Meanwhile, the variable of the level of labor force participation, GDP per capita, has a significant effect on income disparities in Java in 2008-2018. Of all the variables simultaneously influence disparities in Java in 2008-2018. Keywords :  Income disparity; Economic Prosperity; Economic growth;
Incorporating Mindfulness into EFL Literature Courses to Foster Critical Reading
Authors:   University of Mazandaran, Iran, Azizi Mahmoud , Kralik Roman ,   Constantine the Philosopher University in Nitra, Nitra, Slovakia,   Kazan Federal University, Kazan, Russia
Published: December 2020

Recently, educators are becoming interested in exploring the use of mindfulness-based approaches to learning and teaching a foreign language. Through these approaches they tend to reduce stress (for teachers and students alike), enhance and improve classroom atmosphere, and help students to focus their attention, and even think more clearly (Tregenza, 2008; Yager, 2009; Collins et al., 2011). The purpose of the present study was to investigate the impact of mindfulness techniques on the development of critical reading abilities of EFL learners in literature courses. Although much research has been conducted in the realm of mindfulness and positive effects are reported on stress reduction, relaxed environment, and so forth, research on the role of mindfulness in students' critical reading performance is scarce. To this end, a quasi-experimental study was undertaken with the participation of 35 BA students (both male and female) majoring in English language and literature. The participants were divided into two groups of mindful and less mindful students based on their scores on Mindful Attention Awareness Scale (MAAS). Data were collected while students were engaged in some mindfulness techniques before they performed different forms of critical reading activities. A critical reading test was used as pre and posttest to investigate the gain in critical reading ability, if any, after the treatment. Paired-samples T-test through SPSS software was used to analyze the data. The results revealed that there was a significant difference between the mean scores of pre-test and post-test of all the participants. However, since both meditative and socio-cognitive mindfulness practice was utilized in this study, further research is required to shed light on the nature of mindfulness in some new ways and explore the differential effects of these two approaches. Overall, the findings of this study will be beneficial for teachers to be aware of some mindfulness techniques and use them in their classes in order to give students the opportunity of learning in a more relaxed and focused environment.
Evaluation of the effectiveness of integrated biofertilizer in the cultivation of spring wheat in Northern Kazakhstan
Authors: Aipova R. , Abdykadyrova A. Zh. , Kurmanbayev A. A.
Published: December 2020

Based on Plant Growth Promoting Rhizobacteria (PGPR), a biological product for wheat was created and tested in Northern Kazakhstan.
Study of the genetic organization of the strain Rhizobium leguminosarum bv. trifolii forming a symbiosis with clover Trifolium ambiguum
Authors: Aksenova T. S. , Onishchuk O. P. , Kurchak O. N. , Andronov E. E. , Provorov N. A.
Published: December 2020

R. leguminosarum bv. trifolii strains are characterized by narrow host specificity. We have identified a strain that forms nodules on several types of clover and studied the genetic organization of its symbiotic region.
Promising microorganisms for coping herbicide stress in plants
Authors: Bakaeva M. D. , Chetverikov S. P. , Chetverikova D. V. , Kendzhieva A. A.
Published: December 2020

Herbicide-resistant strains of Pseudomonas and Achromobacter have been isolated. Theу are diazotrophic, can mobilize phosphorus from insoluble compounds and synthesize phytohormones in the presence of herbicides.
Influence of the microbiological preparation Atuva + Premax on the formation of soy nodule bacteria
Authors: Barchukova A. Ya. , Chernysheva N. V. , Tosunov Ya. K.
Published: December 2020

Soy is characterized by specific nutrition, the ability to assimilate nitrogen from the air through symbiosis with nodule bacteria. Microbiological preparation Atuva + Premax activates the formation of nodule bacteria.
Characterization of Erwinia amylovora bacteriophages isolated in Belarus
Authors: Besarab N. V. , Golomidova A. K. , Kulikov E. E. , Letarov A. V. , Lagonenko A. L. , Evtushenkov A. N.
Published: December 2020

Erwinia amylovora bacteriophages from the Myoviridae and Podoviridae families were isolated in Belarus. The E. amylovora growth rate was 1.6-7.8 times decreased in the presence of investigated bacteriophages.
Efficient media for high production of microbial lipase from Bacillus subtilis (BSK-L) using response surface methodology for enantiopure synthesis of drug molecules
Authors: Bhushan Indu
Published: December 2020

Lipases are a multipurpose enzyme that holds a significant position in industrial applications due to its ability to catalyse a large number of reactions such as hydrolysis, esterification, interesterification, transesterification which makes it a potential candidate. It is also used for the separation of chiral drugs from the racemic mixture and this property of lipase is considered very important in pharmaceutical industries for the synthesis of enantiopure bioactive molecules. Assuming the tremendous importance of lipases, as stereoselective biocatalysts, in pharmaceuticals and various other commercial applications, industrial enzymologists have been forced to search for those microorganisms which are able to produce novel biocatalysts at reasonably high yield. In the present study microbial lipase was isolated from the water sample of pond at Katra, Jammu and Kashmir (India). This enzyme has shown wide specificity and higher enantioselectivity, which make it pharmaceutical important enzyme. To make it economical for industrial application, it was produced on cheap nutrient media using Response Surface Methodology and got maximum production. It was used for resolution of chiral drugs and the significant results obtained during the course of work shall have potential towards pharmaceutical industries.
Genetically transformed plants growing in Russia: bans and punishments
Authors: Bogatyreva N. V.
Published: December 2020

The authors analyze the Russian system of prohibitions on work with plants obtained by genomic technologies and penalties for their violation. They further conclude there is an imbalance in this system.
The role of heterotrimeric G proteins in the control of the development of symbiosis of leguminous plants with nodule bacteria
Authors: Bovin A. D. , Pavlova O. A. , Kustova D. V. , Leppyanen I. V. , Dolgikh E. A.
Published: December 2020

In this work, we analyzed the localization and effect of suppression of gene expression of β-subunits of G-proteins on nodule formation. The possible interaction of α- and β-subunits with a set of signal regulators in vitro was revealed.
Activation of the symbiosis of free nitrogen-fixing bacteria with plants by an additional influx of photosynthesis products to the roots
Authors: Chikov V. I. , Akhtyamova G. A. , Khamidullina L. A.
Published: December 2020

An in vivo technology is proposed that enhances the export of assimilates from leaves to roots. The possibility of thus increasing the mass of the roots and the formation of additional nitrogen in the soil-plant system is shown.
Study of common wheat lines with genetic material Aegilops speltoides for leaf rust resistance
Authors: Davoyan E. R. , Davoyan R. O. , Zubanova Y. S. , Mikov D. S. , Boldakov D. M.
Published: December 2020

The results of evaluating introgressive lines by resistance to leaf rust and the presence of molecular markers in them linked to the known resistance genes Lr28, Lr35, Lr51, Lr10, Lr26, Lr34 are presented.
RNAi-mutants of Sorghum bicolor (L.) Moench with improved digestibility of kafirins
Authors: Elkonin L. A. , Panin V. M. , Kenzhegulov O. A. , Sarsenova S. Kh.
Published: December 2020

RNAi-mutant with improved kafirin digestibility was obtained in commercial cv. of grain sorghum Avans. In the cv. Zheltozernoe 10, functionally marker-free transgenic plants with improved kafirin digestibility were identified.
Inhibition of the biosynthesis of polyketide mycotoxins by microbial metabolites
Authors: Erokhin D. V. , Mikityuk O. D. , Shcherbakova L. A. , Dzhavakhiya V. G.
Published: December 2020

6-Demethylmevinoliin, a secondary metabolite of Penicillum citrinum, is able to efficiently inhibit the biosynthesis of two polypeptide mycotoxins, aflatoxin B1 and zearalenone, by 92 and 78% of the control, respectively.
The effect of oil pollution on the activity of physiological and biochemical processes in Triticum aestivum L. and the number of rhizospheric microbiota
Authors: Farkhudinov R. G. , Grigoriadi A. S. , Sotnikova Yu. M.
Published: December 2020

The article presents the results of a study of the effect of oil pollution on the biochemical and morphometric parameters of the plant Triticum aestivum L., as well as a change in the number of rhizospheric microorganisms capable of degradation of petroleum hydrocarbons. It was shown that under the influence of pollution in plants increased the activity of redox enzymes. A significant increase in hydrocarbon-oxidizing bacteria and fungi was recorded in the rhizosphere.
The study of transcriptomes of symbiotic tissue of pea using the third-generation sequencing technology Oxford Nanopore
Authors: Gribchenko E. S.
Published: December 2020

The transcriptome profiles the cv. Frisson mycorrhizal roots and inoculated nitrogen-fixing nodules were investigated using the Oxford Nanopore sequencing technology. A database of gene isoforms and their expression has been created.
Analysis of the antibacterial activity of ampicillin by biological sensor based on a microwave resonator
Authors: Guliy O. I. , Zaitsev B. D. , Smirnov A. V. , Karavaeva O. A. , Alsowaidi A. K.M. , Larionova O. S. , Borodina M. A. , Borodina I. A.
Published: December 2020

A method has been developed for analyzing the activity of antibacterial drugs on ampicillin as example by using a biological sensor based on a microwave resonator with an analysis time of ~ 15 minutes.
Biolistic transformation of plants using CRISPR/Cas9 technology
Authors: Gumerova G. , Chemeris A. , Kuluev B.
Published: December 2020

Experiments on CRISPR/Cas9 mediated knock-in approaches in the PDS gene of various genes of interest were planned. Biolistic bombardment mediated delivery of target vectors to plant explants was suggested.
The effect of the buffer zone on the maize pollen flow in mixed crops
Authors: Gusev Yu. S. , Fadeev V. V. , Volokhina I. V. , Zaytsev S. A. , Volkov D. P. , Zuk E. A. , Moiseeva E. M. , Chumakov M. I.
Published: December 2020

Field experiments were carried out to study the effect of the buffer zone length and wind direction on the crossover frequency between the donor and recipient of maize pollen.
Development of fluorescent protein-marked strains of Bacillus subtilis
Authors: Ibragimov A. , Baymiev An. , Lastochkina O.
Published: December 2020

We obtained constructs pHT01-GFP and pHT43-RFP. Recombinant plasmids were transferred into endophytic Bacillus subtilis (strains 10-4 and 26D).
Design of an inducible rolC expression vector
Authors: Khafizova G. V. , Matveeva T. V.
Published: December 2020

rolC is one of the most conserved genes in T-DNA - a fragment, that agrobacteria transfer to the plant during transformation. The rolC functions are not defined yet, to clarify them we designed an inducible expression vector.
Vavilovia formosa rhizobia symbionts belong to Rhizobium leguminosarum bv. viciae species, but form a separate group within it
Authors: Kimeklis А. К. , Aksenova Т. S. , Gladkov G. V. , Kuznetsova I. G. , Sazanova А. L. , Safronova V. I. , Belimov А. А. , Onishchuk О. P. , Kurchak О. N. , Pinaev А. G. , Andronov Е. Е. , Provorov N. А.
Published: December 2020

Ecological isolation, group separation of hkg and sym genes, along with the results of the sterile tube test demonstrate that symbionts of V. formosa belong to R. leguminosarum bv. viciae species, but form a separate group within it.
Development of a scheme for acclimatization of woody plants regenerants using microbial biopesticides
Authors: Konstantinov A. V. , Ostrikova M. Ya. , Kulagin D. V.
Published: December 2020

The effect of isolates and breeding strains on microclonal propagated forest planting material was studied. An increase in survival rate and a stimulating effect in the process of growing ex vitro has been established.
The prospect of using clonal micropropagation to conserve the gene pool of natural Tulipa suaveolens (Liliaceae) populations
Authors: Kritskaya T. A. , Kashin A. S. , Kasatkin M. Yu.
Published: December 2020

Despite the morphological correspondence between the regenerated and natural Tulipa suaveolens plants, the ISSR analysis identified a relatively high degree of somaclonal variability between them.
The “embryo in planta – callus in vitro” system: cytophysiological aspects (by wheat example)
Authors: Kruglova N. N. , Seldimirova O. A.
Published: December 2020

Plant regeneration from calli in vitro is an integral part of a number of biotechnologies. Immature embryos (IE) are particularly promising as explants for obtaining morphogenic calli (MC) in cereals.
Hormonal balance of plants and its relationship with changes in plant growth and productivity under the influence of rhizospheric bacteria
Authors: Kudoyarova G. R. , Arkhipova T. N. , Veselov D. S. , Vysotskaya L. B.
Published: December 2020

Here we analyze the dependence of the growth and water relations on the ability of bacteria to influence the content and distribution of abscisic acid (ABA) in plants under different growing conditions.
Characterization of pea (Pisum sativum L.) microRNAs
Authors: Kulaeva O. A. , Zorin E. A. , Romanyuk D. A. , Gordon M. L. , Gribchenko E. S. , Shtark O. Y. , Afonin A. M. , Tikhonovich I. A. , Zhukov V. A.
Published: December 2020

Pea microRNAs and their targets were identified, and their differential expression was analyzed during the development of symbiosis with rhizobia and mycorrhizal fungi, and under conditions of abiotic stress caused by cadmium.
Plant-microbial fuel cell with using the lettuce during cultivation by panoponic
Authors: Kuleshova T. E. , Galushko A. S. , Gall N. R. , Panova G. G.
Published: December 2020

The experimental plant-microbial fuel cell based on the gradient of bioelectric potentials created in the rhizosphere and compatible with the production of plant products was created and tested.
The influence of malachite green on the level of transcriptional expression of the laccase and DyP-peroxidase genes of the Azospirillum brasilense
Authors: Kupryashina M. A. , Pylaev T. E. , Nikitina V. E.
Published: December 2020

Malachite green (MG), a widely-used and recalcitrant dye, has been confirmed to be carcinogenic and mutagenic against many organisms. Herein, we were aimed at the investigation of the hypothetic role of ligninolytic bacterial enzymes similar to fungal ones in the degradation of synthetic dyes. A multiple increase in the laccases and DyP-peroxidases genes expression level was recorded by RT-qPCR for bacteria of the genus Azospirillum in the presence of MG.
The joint use of strains of microorganisms and natural growth regulators to increase soy resistance to diseases
Authors: Kuznetsova V. A.
Published: December 2020

Growth regulators EcoLarix and ExtraCor together with a biofungicide based on the strain of the fungus Trichoderma viride contributed to the improvement of growth indicators of soybean seedlings healed them from a complex of root rot of various etiologies.
Effect of endophytic Bacillus subtilis on drought stress tolerance of Triticum aestivum L. plants of Steppe Volga and Forest-Steppe West Siberian agroecological groups
Authors: Lastochkina O.
Published: December 2020

Physiological responses of wheat to B. subtilis under drought depends on the belonging of plants to agroecological groups. B. subtilis showed the best positive effect on growth and water status of Steppe Volga agroecological group’s wheat.
Determination of growth-promoting effectiveness of a biopreparation created on Pseudomonas sp. base on wheat plants
Authors: Lukatkin A. A. , Lukatkin A. S.
Published: December 2020

Treatment of wheat seeds with various concentrations of a biopreparation based on Pseudomonas sp. improved the germination and growth of young plants; the best performance was observed at a dilution of 1: 300 (6.3 × 106 CFU / ml).
Endophytic bacteria and plant immunity
Authors: Maksimov I. V. , Shein M. Yu. , Khairullin R. M.
Published: December 2020

Diseases annually cause significant crop losses and reduced quality of agricultural products. The development strategy of new environmentally friendly plant protection products should consider the role of the microbiome in host defense.
Influence of biologicals on photosynthetic pigments in wheat leaves
Authors: Novikova I. I. , Popova E. V. , Kolesnikov L. E. , Kolesnikova Yu. R.
Published: December 2020

Multifunctional biological products based on strains of microorganisms that are antagonists of pathogens and plant disease resistance activators - chitosan and its derivatives increase the content of chlorophyll α and b in flag leaves of wheat, the number and weight of grains in the ear, potential yield, and also reduce the development of yellow rust. The maximum biological effectiveness for these indicators was noted in the experimental version, where Bacillus subtilis VKM B-2604D and B. subtilis VKM B-2605D strains that are part of the Vitaplan biological product and chitosan salicylate (Chitosan II) complex was used.
Transcriptome analysis of Euonymus europaeus fruits at different stages of development
Authors: Pavlenko O. S. , Sadovskaya N. S. , Mustafayev O. N. , Akashkina Yu. V. , Goldenkova-Pavlova I. V.
Published: December 2020

The transcript Euonymus europaeus was collected de novo. A comparative analysis of transcriptomes from the stage of the globular embryo and the mature fruit made it possible to identify key DGAT genes at the contrasting stages of the development of E. europaeus fruits.
Investigation of molecular mechanisms involved in Erwinia amylovora biofilm formation
Authors: Pesotskaya K. Yu. , Lagonenko A. L. , Evtushenkov A. N.
Published: December 2020

In this study we generated four insertional Erwinia amylovora mutants within bssS gene and waa, aaeXAB and hec operons. Our research included studying of the phenotypic characteristics of these mutants.
Bioengineering of archaeal flagella
Authors: Pyatibratov M. G. , Syutkin A. S. , Beznosov S. N. , Galeva A. V. , Shchyogolev S. Yu.
Published: December 2020

It was shown that the Haloferax volcanii flagella assembly system can accept alien flagellins and build functional recombinant flagella. The results can be used for targeted flagella modification to create multifunctional nanomaterials.
New bacterial strains Рseudomonas, promising for agricultural biotechnology
Authors: Rafikova G. F. , Kuzina E. V.
Published: December 2020

The bacteria P. laurentiana АНТ 17 and P. laurentiana АНТ 56 have plant-beneficial properties: its exhibit antagonistic activity against phytopathogenic micromycetes, are capable of decomposing phosphates and synthesizing phytohormones.
Studying the kinetics of salicin hydrolysis in an acidic environment
Authors: Sabutova A. B.
Published: December 2020

The work is devoted to the study of the kinetics of the hydrolysis of salicin in aqueous solutions. To determine salicin in the objects of study, the HPLC method was used. During the study, it was found that the hydrolysis of salicin occurs only in aqueous solutions of strong acids.
The effect of Fusarium culmorum and Pseudomonas fluorescens 2137 on the content of abscisic acid in the roots and shoots of barley seedlings
Authors: Shaposhnikov A. I. , Vishnevskaya N. A. , Shakhnazarova V. Yu. , Syrova D. S. , Borodina E. V. , Kovaleva O. N. , Strunnikova O. K.
Published: December 2020

Inoculation of barley with a F. culmorum not accompanied by an increase in the number of abscisic acid in plants, but under P. fluorescens 2137 inoculation, the accumulation of abscisic acid in the roots occurred.
Variability of actinomycete complexes in the rhizosphere of transgenic and intact tobacco lines
Authors: Shirokikh I. G. , Nazarova Ya. I.
Published: December 2020

The results on the detection of changes in model microbial complexes formed in the rhizosphere of transgenic tobacco plants with enhanced antioxidant (FeSOD 1) and osmoprotective (codA) protection are presented.
Specific cytochromes P450 and adrenodoxin-like mitochondrial ferredoxins as components of the progesterone hormone system of higher plants involved in comprehensive protection from biotic and abiotic stresse
Authors: Shpakovski D. G. , Shematorova E. K. , Babak O. G. , Slovokhotov I. Yu. , Spivak S. G. , Khaliluev M. R. , Doludin Yu. V. , Klykov V. N. , Baranova E. N. , Tereshonkova T. A. , Kilchevsky A. V. , Shpakovski G. V.
Published: December 2020

In plants, along with the brassinosteroid, the older progesterone hormone system also functions. We have characterized some components of this system and shown its importance for enhancing plant immunity.
Metabolic alterations in pea leaves and roots during arbuscular mycorrhiza development
Authors: Shtark O. Y. , Puzanskiy R. K. , Avdeeva G. S. , Yemelyanov V. V. , Kliukova M. S. , Shavarda A. L. , Kirpichnikova A. A. , Afonin A. M. , Tikhonovich I. A. , Zhukov V. A. , Shishova M. F.
Published: December 2020

A shift in the metabolic profiles of leaves and roots of mycorrhized pea plants towards the profiles of control plants at earlier stages of development was revealed. Thus, mycorrhization led to the retardation of plant development.
In vivo callus formation on the surface of tubers of Manchu tubergourd (Thladiantha dubia, Cucurbitaceae)
Authors: Shvets D. Yu. , Kuluev B. R.
Published: December 2020

For the first time, shingles of callus-like structures were found in Thladiantha dubia. Of these formations, shoots and roots are intensively regenerated in vivo. Such a mechanism underlies the active vegetative propagation and wide distribution of this invasive species.
Study of the kinetics of sorption of heavy metal ions by dry biomass of cyanobacteria
Authors: Skugoreva S. G. , Kantor G. Ya. , Domracheva L. I.
Published: December 2020

Sorption of copper (II), lead (II) and cadmium ions from a solution with a concentration of 1 ∙ 10-4 mol/L by dry biomass of cyanobacteria Fischerella muscicola occurred 1.4-1.9 times faster than Nostoc paludosum. The sorption rate of lead and copper ions was 1.9-2.6 times higher compared to cadmium ions.
Engineering of vectors essential to derive chimeric proteins based on superfolder green fluorescent protein and harpins
Authors: Tchebotarev L. , Valentovich L.
Published: December 2020

Conjugation of harpins with green fluorescent protein is aimed at achieving enhanced solubility and stability of chimeric protein, facilitating qualitative and quantitative evaluation of its expression in the routine experiments.
Biological activity of complexes based on polycarbonyl ligands:
assessment of the mode of action using molecular docking
Authors: Tobysheva P. D. , Khamidullina L. A. , Puzyrev I. S. , Pestov A. V.
Published: December 2020

The binding ability towards protein biomolecular targets have been analyzed for metal complexes based on polycarbonyl ligands using molecular docking.
Symbiotic nodule development
Authors: Tsyganov V. E.
Published: December 2020

The interaction of legumes with rhizobia leads to formation of the symbiotic nodules on their roots, which are specialized plant organs for nitrogen fixation. Considerable progress has been made in deciphering the molecular-genetic and cellular mechanisms of symbiotic nodule development in recent years. However, some aspects of nodule development clearly merit much more attention.
Film coatings based on exopolysaccharides of lactic acid bacteria and their use
Authors: Uryadova G. T. , Fokina N. A. , Karpunina L. V.
Published: December 2020

It is shown that film coatings based on the exopolysaccharides of lactic acid bacteria Lactococcus lactis B-1662 and Streptococcus thermophilus contribute to the healing of burns in rats.
Sequencing and analysis of genomes of bacteria used to protect plants and animals from diseases
Authors: Valentovich L. N. , Muratova A. A. , Shavela Yu. V. , Sikolenko M. A. , Akhremchuk A. E.
Published: December 2020

The main stages of work with perspective strains for protecting plants and animals from diseases are presented: genome sequencing and analysis, genetic construction of improved strains and checking their survival under model conditions.
Comparative analysis of transcriptomes of different morphological structures of the basidiomycete Lentinus edodes
Authors: Vetchinkina E. P. , Gorshkov V. Yu. , Gogoleva N. E. , Gogolev Yu. V. , Nikitina V. E.
Published: December 2020

A comparative analysis of transcriptomes at the vegetative and generative stages of the development of Lentinus edodes basidiomycetes was carried out. The nature of differential gene expression was described, and the activated/repressed metabolic pathways were visualized.
Efficiency of Pseudomonas aureofaciens colonization in wheat rhizosphere under model condition
Authors: Vlasova A. I. , Minaeva O. M.
Published: December 2020

The survival of Pseudomonas aureofaciens in the wheat rhizosphere was studied. A significant increase in the number of pseudomonads and tetracycline resistant bacteria was shown when introduced into the model system of strains.
Agrobacterium tumefaciens - associative microsymbionts of grain crop
Authors: Yakubovskaya A. I. , Kameneva I. A. , Melnichuk T. N. , Matveeva T. V. , Gritchin M. V. , Abdyrashitov S. F.
Published: December 2020

The installed absence of Ti-plasmids in the genome structure of strains of diazotrophic bacteria Agrobacterium tumefaciens 32 and A. tumefaciens P3 was found, which characterizes the strains as non-pathogenic. Bacterization of grain seeds by associative bacterial strains stimulates root development by up to 40%.
Study of the garden pea (Pisum sativum L.) symbioses in post-genomic era
Authors: Zhukov V. A. , Afonin A. M. , Akhtemova G. A. , Bovin A. D. , Dolgikh A. V. , Gorshkov A. P. , Gribchenko E. S. , Ivanova K. A. , Kirienko A. N. , Kitaeva A. B. , Kliukova M. S. , Kulaeva O. A. , Kusakin P. G. , Leppyanen I. V. , Pavlova O. A. , Romanyuk D. A. , Serova T. A. , Shtark O. Y. , Sulima A. S. , Tsyganova A. V. , Vasileva E. N. , Zhernakov A. I. , Dolgikh E. A. , Tsyganov V. E. , Tikhonovich I. A.
Published: December 2020

Mutualistic symbioses formed by garden pea have been studied with use of ‘omic’ technologies in order to gain a new understanding of molecular mechanisms of beneficial effect that microsymbionts have on seed yield and quality.
Keywords: garden pea, transcriptomics, nitrogen fixation, arbuscular mycorrhiza, PGPB
Effect of bacterization with Pseudomonas extremorientalis PhS1 on photosynthesis processes in wheat plants
(Triticum aestivum L.) under phytopathogenic load
Authors: Zyubanova T. I. , Minaeva O. M. , Tereshchenko N. N.
Published: December 2020

We found that bacterization of wheat with Pseudomonas extremorientalis increases the efficiency of open photosystem II reaction centers and reduces the photosynthesis losses dissipated as heat in the presence of a phytopathogen.
TÜKETİCİLERİN KREDİ KARTINA YÖNELİK TUTUMU İLE FİNANSAL OKURYAZARLIK DÜZEYİ ARASINDAKİ İLİŞKİ
Authors: KÖYLÜOĞLU A. Selçuk , DOĞAN Mesut
Published: December 2020

Kredi kartları kolayda ürünlerden endüstriyel ürünlere kadar pek çok kalemde kullanılmaktadır. Dolayısıyla günümüz tüketicisinin neredeyse vazgeçemediği bir ödeme aracı haline gelmiştir. Ancak kredi kartı kullanımı pek çok konuyla ilişkili olduğu için bireyler bazında farklılıklar göstermektedir. İlişki olduğu konulardan bir tanesi de finansal okuryazarlıktır. Finansal okuryazarlık kısaca, finansal konularda bilgi, beceri ve finansal davranışlarda rasyonelliği sağlama derecesi şeklinde ifade edilebilir. Bu doğrultuda bu çalışmanın amacı, tüketicilerin kredi kartına karşı tutumları ve finansal okuryazarlık seviyeleri arasındaki ilişkiyi tespit etmektir. Bu amaçla Ankara’da kredi kartı kullanan 600 kişi üzerinde anket gerçekleştirilmiştir. Yapılan analiz sonucunda bireylerin kredi kartı kullanım adedi azaldıkça katılımcıların finansal okuryazarlık seviyeleri arttığı tespit edilmiştir. Ayrıca katılımcıların finansal okuryazarlık seviyeleri ile kredi kartı tutumu arasında pozitif ve istatistiksel olarak anlamlı bir ilişki belirlenmiştir.