bluuebunny/crossref_metadata_2025 huggingface dataset:
Models of the Translation Process and the Free Energy Principle
Authors: Carl Michael
Published: June 2023

Translation process research (TPR) has generated a large number of models that aim at explaining human translation processes. In this paper, I suggest an extension of the monitor model to incorporate aspects of relevance theory (RT) and to adopt the free energy principle (FEP) as a generative model to elucidate translational behaviour. The FEP—and its corollary, active inference—provide a general, mathematical framework to explain how organisms resist entropic erosion so as to remain within their phenotypic bounds. It posits that organisms reduce the gap between their expectations and observations by minimising a quantity called free energy. I map these concepts on the translation process and exemplify them with behavioural data. The analysis is based on the notion of translation units (TUs) which exhibit observable traces of the translator’s epistemic and pragmatic engagement with their translation environment, (i.e., the text) that can be measured in terms of translation effort and effects. Sequences of TUs cluster into translation states (steady state, orientation, and hesitation). Drawing on active inference, sequences of translation states combine into translation policies that reduce expected free energy. I show how the notion of free energy is compatible with the concept of relevance, as developed in RT, and how essential concepts of the monitor model and RT can be formalised as deep temporal generative models that can be interpreted under a representationalist view, but also support a non-representationalist account.
Skill Mismatch, Nepotism, Job Satisfaction, and Young Females in the MENA Region
Authors: Arayssi Mahmoud , Fakih Ali , Haimoun Nathir
Published: June 2023

Skills utilization is an important factor affecting labor productivity and job satisfaction. This paper examines the effects of skills mismatch, nepotism, and gender discrimination on wages and job satisfaction in MENA workplaces. Gender discrimination implies social costs for firms due to higher turnover rates and lower retention levels. Young females suffer disproportionality from this than their male counterparts, resulting in a wider gender gap in the labor market at multiple levels. Therefore, we find that the skill mismatch problem appears to be more significant among specific demographic groups, such as females, immigrants, and ethnic minorities; it is also negatively correlated with job satisfaction and wages. We bridge the literature gap on youth skill mismatch’s main determinants, including nepotism, by showing evidence from some developing countries. Given the implied social costs associated with these practices and their impact on the labor market, we have compiled a list of policy recommendations that the government and relevant stakeholders should take to reduce these problems in the workplace. Therefore, we provide a guide to address MENA’s skill mismatch and improve overall job satisfaction.
A Novel Cloud Enabled Access Control Model for Preserving the Security and Privacy of Medical Big Data
Authors: Alabdulatif Abdullah , Thilakarathne Navod Neranjan , Kalinaki Kassim
Published: June 2023

In the context of healthcare, big data refers to a complex compilation of digital medical data collected from many sources that are difficult to manage with normal technology and software due to its size and complexity. These big data are useful in various aspects of healthcare, such as disease diagnosis, early prevention of diseases, and predicting epidemics. Even though medical big data has many advantages and a lot of potential for revolutionizing healthcare, it also has a lot of drawbacks and problems, of which security and privacy are of the utmost concern, owing to the severity of the complications once the medical data is compromised. On the other hand, it is evident that existing security and privacy safeguards in healthcare organizations are insufficient to protect their massive, big data repositories and ubiquitous environment. Thus, motivated by the synthesizing of the current knowledge pertaining to the security and privacy of medical big data, including the countermeasures, in the study, firstly, we provide a comprehensive review of the security and privacy of medical big data, including countermeasures. Secondly, we propose a novel cloud-enabled hybrid access control framework for securing the medical big data in healthcare organizations, and the result of this research indicates that the proposed access control model can withstand most cyber-attacks, and it is also proven that the proposed framework can be utilized as a primary base to build secure and safe medical big data solutions. Thus, we believe this research would be useful for future researchers to comprehend the knowledge on the security and privacy of medical big data and the development of countermeasures.
Growth Response of Norway Spruce (Picea abies [L.] Karst.) in Central Bohemia (Czech Republic) to Climate Change
Authors: D’Andrea Giuseppe , Šimůnek Václav , Pericolo Osvaldo , Vacek Zdeněk , Vacek Stanislav , Corleto Roberto , Olejár Lukáš , Ripullone Francesco
Published: June 2023

Norway spruce (Picea abies (L.) Karst.) is a significant conifer tree species in Europe that holds significant economic and ecological value. However, it remains one of the most sensitive to climate change. This study describes the climate–growth relationship, focusing on dendroecology in hilly spruce forests (319–425 m a.s.l.) located in Bohemia, the Czech Republic, during 1950–2018. The results confirmed that the highest radial increment was obtained in locations with higher precipitation (Kostelec), while the lowest growth was observed in locations with lower precipitation (Karlstejn). Tree-ring growth shows very low increments for the years 1964 and 1976 for all plots, and the years with the least growth were confirmed by the negative pointer year analysis. This study confirmed precipitation as the main factor that affects the growth of spruce at lower altitudes. The radial growth for all study sites shows a statistically significant positive correlation with precipitation during the growing season, while no statistically significant values between radial growth and temperature were obtained. This study demonstrates that Norway spruce is affected more by precipitation than temperature, and the results indicate that this conifer is seriously affected by the lack of precipitation at lower altitudes in the Czech Republic, where the species is not native.
Effect of Samples Length on the Characteristics of Moisture Transfer and Shrinkage of Eucalyptus urophylla Wood during Conventional Drying
Authors: Liu Honghai , Ke Mengqing , Zhou Ting , Sun Xinlu
Published: June 2023

Moisture transfer influences wood deformation and moisture content (MC) distribution during conventional drying of Eucalyptus urophylla wood. This study aims to investigate the effect of sample length (30, 100, and 200 mm) on moisture distribution and transfer in different directions and locations and on deformation of wood. The results showed that when the MC was above the fiber saturated point (FSP), the drying rate decreases exponentially with an increase of sample length; however, below the FSP, there was no obvious relationship between the drying rate and sample length and above the FSP, the moisture distribution was non-uniform along tangential, radial, and longitudinal directions and became even below the FSP, which was more significant in the middle location of wood. The greatest MC differences occurred between the surface and sub-central layers along the tangential and radial direction, which were between the end and sub-middle locations along the longitudinal direction. The effect of sample length on the MC distribution and MC differences along wood in the three directions depended on locations and the MC stage of wood; most of the free water and bound water transferred from the wood central to the ends along the longitudinal direction for three sets of samples. Bound water diffusion significantly slowed as the sample length exceeded 200 mm; sample length affects wood collapse and its recovery, but the drying rate has a lesser effect on collapse for samples with a length below 200 mm.
Study on the Effect of Acrylic Acid Emulsion on the Properties of Poplar Wood Modified by Sodium Silicate Impregnation
Authors: Zhang Yuan , Guan Pengfei , Ma Xin , Li Ping , Sun Zhenyu , Li Xianjun , Zuo Yingfeng
Published: June 2023

Inorganic silicate impregnation-modified fast-growing wood shows improved mechanical properties and thermal stability, but inorganic silicate agent loss and moisture absorption affect its processability. This study proposes a method to improve the impregnating agent loss and modified wood moisture absorption of poplar wood modified by using an acrylic acid emulsion/sodium silicate composite. The acrylic acid emulsion coated the sodium silicate and cell wall surfaces with a cured film that blocked water molecules from entering the modified wood. The acrylic acid emulsion adhered to the wood and sodium silicate, thus reducing impregnating agent loss. The addition of the acrylic acid emulsion maintained the excellent mechanical properties of sodium silicate-modified poplar wood and greatly improved its bending strength. The water absorption and moisture swelling rate were significantly decreased, and the dimensional stability of modified poplar wood was more than 50% higher than that of unmodified poplar wood. The thermogravimetric analysis (TG)results showed that the addition of organic components reduced the heat resistance of modified wood, but the thermal stability was still higher than unmodified wood. Fourier-transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS) results showed that acrylic esters in acrylic acid emulsion reacted with hydroxyl groups on sodium silicate and wood to form covalent bonds that improved the impregnating agent’s resistance to loss and reduced the moisture absorption of the wood. The modified poplar wood showed better dimensional stability and water resistance.
BERT4Loc: BERT for Location—POI Recommender System
Authors: Bashir Syed , Raza Shaina , Misic Vojislav
Published: June 2023

Recommending points of interest (POI) is a challenging task that requires extracting comprehensive location data from location-based social media platforms. To provide effective location-based recommendations, it is important to analyze users’ historical behavior and preferences. In this study, we present a sophisticated location-aware recommendation system that uses Bidirectional Encoder Representations from Transformers (BERT) to offer personalized location-based suggestions. Our model combines location information and user preferences to provide more relevant recommendations compared to models that predict the next POI in a sequence. Based on our experiments conducted on two benchmark datasets, we have observed that our BERT-based model surpasses baselines models in terms of HR by a significant margin of 6% compared to the second-best performing baseline. Furthermore, our model demonstrates a percentage gain of 1–2% in the NDCG compared to second best baseline. These results indicate the superior performance and effectiveness of our BERT-based approach in comparison to other models when evaluating HR and NDCG metrics. Moreover, we see the effectiveness of the proposed model for quality through additional experiments.
A Refinement of Backward Correlation Technique for Precise Brillouin Frequency Shift Extraction
Authors: Barkov Fedor L. , Krivosheev Anton I. , Konstantinov Yuri A. , Davydov Andrey R.
Published: June 2023

A new method for extracting the Brillouin frequency shift (BFS) from the Brillouin gain spectrum (BGS), the modified backward correlation method (MBWC), is presented. The possibilities of using MBWC, and MBWC in combination with the Lorentzian curve fitting (LCF) based on Levenberg–Marquardt (LM) method, are studied. The effectiveness of the new method, and its combination with LM, has been demonstrated for processing spectra with a low signal-to-noise ratio (SNR). The experiments, which were in good agreement with the performed simulation, showed that at SNR = 0 dB, the combined (MBWC + LM) method provided the BFS extraction error of less than 4 MHz, while the state-of-the-art LM algorithm extracted it with the error greater than 4.5 MHz. The advantage of correlation methods becomes more significant with the decreasing SNR: at SNR = −2 dB, the LM’s error is 14.3 MHz, and that of the combined one is 8.1 MHz.
Food Front-of-Pack Labelling and the Nutri-Score Nutrition Label—Poland-Wide Cross-Sectional Expert Opinion Study
Authors: Panczyk Mariusz , Dobrowolski Hubert , Sińska Beata I. , Kucharska Alicja , Jaworski Mariusz , Traczyk Iwona
Published: June 2023

Front-of-pack labelling (FOPL) systems, such as the Nutri-Score, play a crucial role in promoting healthy diets and raising consumer awareness. Our study aimed to gather the opinions of Polish experts on the Nutri-Score and its relation to an ideal information system. We conducted a Poland-wide expert opinion study using a cross-sectional design survey with 75 participants, who had an average of 18 ± 13 years of experience and were mainly employed at medical and agricultural universities. The data were collected with the CAWI method. The results showed that the most important features of an FOPL system were clarity, simplicity, consistency with healthy eating recommendations, and the ability to objectively compare products within the same group. While more than half of the respondents believed that the Nutri-Score provides an overall assessment of a product’s nutritional value and facilitates quick purchasing decisions, it falls short in helping consumers compose a balanced diet and cannot be applied to all product groups. The experts also expressed concerns about the system’s ability to account for a product’s degree of processing, full nutritional value and carbon footprint. In conclusion, Poland’s current labelling system needs expansion, but the Nutri-Score requires significant and detailed changes and validation against national guidelines and expert expectations before implementation.
Green Processing Technology of Meat and Meat Products
Authors: Zhou Changyu , Cao Jinxuan
Published: June 2023

Consumers are increasingly demanding higher quality meat products [...]
The Effect of Asymmetrical Occlusion on Surface Electromyographic Activity in Subjects with a Chewing Side Preference: A Preliminary Study
Authors: Zhang Yubing , Liu Kun , Shao Zhengwei , Lyu Chengqi , Zou Derong
Published: June 2023

The relationship between asymmetrical occlusion and surface electromyographic activity (sEMG) in people with different chewing preferences is not clear. In this study, the 5 s sEMG changes in the masseter muscle (MM), sternocleidomastoid (SCM), lateral (LGA), and medial (MGA) gastrocnemius muscles were recorded in controls, and subjects with chewing side preference (CSP) during clench with bilateral (BCR), left (LCR), and right (RCR) posterior teeth placement of cotton rolls. The images of the middle 3 s were selected and expressed as the root mean square (unit: μV/s). The EMG waves of bilateral muscles were compared by computing the percentage overlapping coefficient (POC). Only the POCMM of the CSP showed gender differences at BCR and RCR. Between the control group and the CSP group, there were significant differences in the POCMM and the POCLGA at BCR. In addition, there was a significant difference in POCMM and POCSCM between the two populations in different occlusal positions. The change in the POCSCM correlated with the change in the POCMM (r = 0.415, p = 0.018). The experiment-induced asymmetrical occlusion showed that the altered symmetry of the MM correlated with the altered symmetry of the SCM. Long-term asymmetrical occlusion (i.e., CSP) not only affects MM but also has potential effects on other superficial muscles (e.g., LGA).
Unveiling Neurovascular Orofacial Pain: An Underdiagnosed Form of Chronic Orofacial Pain
Authors: Haviv Yaron
Published: June 2023

Neurovascular orofacial pain (NVOP) is a relatively rare type of facial pain syndrome that shares similarities with migraine, orofacial migraine, and trigeminal autonomic cephalalgias (TACs) [...]
Tricuspid Valve Endocarditis Due to Methicillin-Resistant Staphylococcus aureus in a Previously Healthy Young Patient without a Drug Abuse History: A Case Report and a Review of the Literature
Authors: Andrijašević Nataša , Perešin Vranjković Martina , Dobrović Karolina , Pristaš Irina , Andrašević Saša , Tambić Andrašević Arjana
Published: June 2023

Right-sided infective endocarditis due to methicillin-resistant Staphylococcus aureus (MRSA) is strongly associated with intravenous drug abuse, congenital heart disease, or previous medical treatment and is rare in healthy patients without a history of drug abuse. Here, we present a case of an 18-year-old male with no drug abuse history and no medical burden who was diagnosed with MRSA tricuspid valve endocarditis. Due to initial symptoms which indicated community-acquired pneumonia and radiological finding of interstitial lesions, empiric therapy with ceftriaxone and azithromycin was started. After the detection of Gram-positive cocci in clusters in several blood culture sets, endocarditis was suspected, and flucloxacillin was added to the initial therapy. As soon as methicillin resistance was detected, the treatment was switched to vancomycin. Transesophageal echocardiography established the diagnosis of right-sided infective endocarditis. A toxicological analysis of hair was carried out, and no presence of narcotic drugs was found. After six weeks of therapy, the patient was fully recovered. Exceptionally, tricuspid valve endocarditis can be diagnosed in previously healthy people who are not drug addicts. As the clinical presentation commonly resembles a respiratory infection, a misdiagnosis is possible. Although MRSA rarely causes community-acquired infections in Europe, clinicians should be aware of this possibility.
Pain Self-Management with Inhaled Methoxyflurane by Emergency Department Trauma Patients: A Prospective, Interventional, Single-Center Study
Authors: Ghazali Daniel Aiham , Bouzid Donia , Frachon Alix , Ait-Abdesselam Sarah , Kenway Philippe , Choquet Christophe , Casalino Enrique
Published: June 2023

The coronavirus disease 2019 (COVID-19) pandemic has led to overcrowding in many emergency departments (EDs). The present single-center, prospective, interventional study (conducted at Bichat University Medical Center (Paris, France)) was designed to assess the impact of self-administered, inhaled, low-dose methoxyflurane on trauma pain in a pre-ED fast-track zone dedicated to the management of lower-acuity non-COVID-19 patients. In the first phase of the study, the control group consisted of patients with mild-to-moderate trauma pain, for whom the triage nurse initiated pain management (based on the World Health Organization (WHO)’s analgesic ladder). In the second phase, the intervention group consisted of similar patients who self-administered methoxyflurane as an adjuvant to the standard analgesic ladder. The primary endpoint was the numerical pain rating scale (NPRS) score (from 0 to 10) recorded at different time points during the patient’s care (T0: arrival in the ED, T1: exit from the triage box, T2: in the radiology department, T3: clinical examination, and T4: discharge from the ED). The level of agreement between the NPRS and the WHO analgesic ladder was assessed by the calculation of Cohen’s kappa. Pairwise comparisons of continuous variables were performed with Student’s t-test or a non-parametric Mann–Whitney U test. Changes over time in the NPRS were analyzed in an analysis of variance (with Scheffe’s post hoc test if a pairwise comparison was significant) or a non-parametric Kruskal–Wallis H test. In all, 268 and 252 patients were included in the control and intervention groups, respectively. The two groups had similar characteristics. The level of agreement between the NPRS score and the analgesic ladder was high in both the control and intervention groups (Cohen’s kappa: 0.74 and 0.70, respectively). The NPRS score decreased significantly between T0 and T4 in both groups (p < 0.001), but the decrease between T2 and T4 was significantly greater in the intervention group (p < 0.001). The proportion of patients still in pain on discharge was significantly lower in the intervention group than in the control group (p = 0.001). In conclusion, a combination of self-administered methoxyflurane and the WHO analgesic ladder improves pain management in the ED.
Obesity Status and Physical Fitness Levels in Male and Female Portuguese Adolescents: A Two-Way Multivariate Analysis
Authors: Encarnação Samuel , Rodrigues Filipe , Monteiro António Miguel , Gouili Hatem , Hattabi Soukaina , Sortwell Andrew , Branquinho Luís , Teixeira José Eduardo , Ferraz Ricardo , Flores Pedro , Silva-Santos Sandra , Ribeiro Joana , Batista Amanda , Forte Pedro Miguel
Published: June 2023

Obesity and decreasing fitness levels among the youth are growing concerns in Portugal, similar to other developed countries, with implications for health and psychomotor development. Understanding the influence of health determinants such as sex and age are crucial for developing effective public health strategies. This study aimed to analyze the association between sex and chronological age with obesity status and physical fitness in Portuguese adolescents. A total of 170 adolescents (85 males and 85 females) were evaluated for body mass index, abdominal adiposity, aerobic fitness, abdominal resistance, upper limb resistance, lower limb power, and maximal running speed in a 40 m sprint using the FITescola® physical fitness battery, a Portuguese government initiative. The general model, analyzed using Pillai’s trace, showed a significant effect of age and sex on body mass index, abdominal circumference, aerobic fitness, abdominal resistance, upper limb resistance, lower limb power, and maximal running speed (V = 0.99, F (7) = 10,916.4, p < 0.001, partial η2, sex = 0.22; age = 0.43, sex and age interaction = 0.10). Boys had higher physical fitness levels than girls in most tests, but both sex groups had a significantly higher proportion of non-fit adolescents, with boys showing the highest number of participants classified as non-fit.
Human Sperm Head Vacuoles Are Related to Nuclear-Envelope Invaginations
Authors: Gómez-Torres María José , Luna-Romero Javier , Fernández-Colom Pedro José , Aizpurua Jon , Avilés Manuel , Romero Alejandro
Published: June 2023

Nuclear vacuoles are specific structures present on the head of the human sperm of fertile and non-fertile men. Human sperm head vacuoles have been previously studied using motile sperm organelle morphology examination (MSOME) and their origin related to abnormal morphology, abnormal chromatin condensation and DNA fragmentation. However, other studies argued that human sperm vacuoles are physiological structures and consequently, to date, the nature and origin of the nuclear vacuoles remains to be elucidated. Here, we aim to define the incidence, position, morphology and molecular content of the human sperm vacuoles using transmission electron microscopy (TEM) and immunocytochemistry techniques. The results showed that ~50% of the analyzed human sperm cells (n = 1908; 17 normozoospermic human donors) contained vacuoles mainly located (80%) in the tip head region. A significant positive correlation was found between the sperm vacuole and nucleus areas. Furthermore, it was confirmed that nuclear vacuoles were invaginations of the nuclear envelope from the perinuclear theca and containing cytoskeletal proteins and cytoplasmic enzyme, discarding a nuclear or acrosomal origin. According to our findings, these human sperm head vacuoles are cellular structures originating from nuclear invaginations and contain perinuclear theca (PT) components, allowing us to define a new term of ‘nuclear invaginations’ rather than ‘nuclear vacuoles’.
High Plasma Angiopoietin-2 Levels Predict the Need to Initiate Dialysis within Two Years in Patients with Chronic Kidney Disease
Authors: Szymczak Anna , Kusztal Mariusz , Gołębiowski Tomasz , Letachowicz Krzysztof , Goździk Anna , Kościelska-Kasprzak Katarzyna , Tukiendorf Andrzej , Krajewska Magdalena
Published: June 2023

Volume status, congestion, endothelial activation, and injury all play roles in glomerular filtration rate (GFR) decline. In this study, we aimed to determine whether the plasma endothelial and overhydration markers could serve as independent predictors for dialysis initiation in patients with chronic kidney disease (CKD) 3b-5 (GFR < 45 mL/min/1.72 m2) and preserved ejection fraction. A prospective, observational study in a single academic center was conducted from March 2019 to March 2022. Plasma levels of angiopoietin (Ang)-2, Vascular Endothelial Growth Factor-C (VEGF-C), Vascular Cell Adhesion Molecule-1 (VCAM-1), Copeptin (CPP), beta-trace protein (BTP), brain natriuretic peptide (BNP), and cardiac troponin I (cTnI) were all measured. Lung ultrasound (US) B-lines, bioimpedance, and echocardiography with global longitudinal strain (GLS) were recorded. The study outcome was the initiation of chronic dialysis (renal replacement therapy) during 24 months of follow-up. A total of 105 consecutive patients with a mean eGFR of 21.3 mL/min/1.73 m were recruited and finally analyzed. A positive correlation between Ang-2 and VCAM-1 and BTP was observed. Ang-2 correlated positively with BNP, cTnI, sCr, E/e′, and the extracellular water (ECW)/intracellular water (ICW) ratio (ECW/ICW). After 24 months, a deterioration in renal function was observed in 47 patients (58%). In multivariate regression analysis, both VCAM-1 and Ang-2 showed independent influences on risk of renal replacement therapy initiation. In a Kaplan-Meier analysis, 72% of patients with Ang-2 concentrations below the median (3.15 ng/mL) survived without dialysis for two years. Such an impact was not observed for GFR, VCAM, CCP, VEGF-C, or BTP. Endothelial activation, quantified by plasma levels of Ang-2, may play a key role in GFR decline and the need for dialysis initiation in patients with CKD 3b, 4, and 5.
Functional Characterization of Mouse and Human Arachidonic Acid Lipoxygenase 15B (ALOX15B) Orthologs and of Their Mutants Exhibiting Humanized and Murinized Reaction Specificities
Authors: Kakularam Kumar R. , Canyelles-Niño Miquel , Chen Xin , Lluch José M. , González-Lafont Àngels , Kuhn Hartmut
Published: June 2023

The arachidonic acid lipoxygenase 15B (ALOX15B) orthologs of men and mice form different reaction products when arachidonic acid is used as the substrate. Tyr603Asp+His604Val double mutation in mouse arachidonic acid lipoxygenase 15b humanized the product pattern and an inverse mutagenesis strategy murinized the specificity of the human enzyme. As the mechanistic basis for these functional differences, an inverse substrate binding at the active site of the enzymes has been suggested, but experimental proof for this hypothesis is still pending. Here we expressed wildtype mouse and human arachidonic acid lipoxygenase 15B orthologs as well as their humanized and murinized double mutants as recombinant proteins and analyzed the product patterns of these enzymes with different polyenoic fatty acids. In addition, in silico substrate docking studies and molecular dynamics simulation were performed to explore the mechanistic basis for the distinct reaction specificities of the different enzyme variants. Wildtype human arachidonic acid lipoxygenase 15B converted arachidonic acid and eicosapentaenoic acid to their 15-hydroperoxy derivatives but the Asp602Tyr+Val603His exchange murinized the product pattern. The inverse mutagenesis strategy in mouse arachidonic acid lipoxygenase 15b (Tyr603Asp+His604Val exchange) humanized the product pattern with these substrates, but the situation was different with docosahexaenoic acid. Here, Tyr603Asp+His604Val substitution in mouse arachidonic acid lipoxygenase 15b also humanized the specificity but the inverse mutagenesis (Asp602Tyr+Val603His) did not murinize the human enzyme. With linoleic acid Tyr603Asp+His604Val substitution in mouse arachidonic acid lipoxygenase 15b humanized the product pattern but the inverse mutagenesis in human arachidonic acid lipoxygenase 15B induced racemic product formation. Amino acid exchanges at critical positions of human and mouse arachidonic acid lipoxygenase 15B orthologs humanized/murinized the product pattern with C20 fatty acids, but this was not the case with fatty acid substrates of different chain lengths. Asp602Tyr+Val603His exchange murinized the product pattern of human arachidonic acid lipoxygenase 15B with arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. An inverse mutagenesis strategy on mouse arachidonic acid lipoxygenase 15b (Tyr603Asp+His604Val exchange) did humanize the reaction products with arachidonic acid and eicosapentaenoic acid, but not with docosahexaenoic acid.
Role of CIV NS1 Protein in Innate Immunity and Viral Replication
Authors: Fu Cheng , Zhu Wenhui , Cao Nan , Liu Wenjun , Lu Zhier , Wong Ziyuan , Guan Kaiting , Hu Chunyan , Han Baoting , Zeng Sen , Fan Shuangqi
Published: June 2023

The innate immune pathway serves as the first line of defense against viral infections and plays a crucial role in the host’s immune response in clearing viruses. Prior research has indicated that the influenza A virus has developed various strategies to avoid host immune responses. Nevertheless, the role of the NS1 protein of the canine influenza virus (CIV) in the innate immune pathway remains unclear. In this study, eukaryotic plasmids of NS1, NP, PA, PB1, and PB2 were constructed, and it was found that these proteins interact with melanoma differentiation-associated gene 5 (MDA5) and antagonize the activation of IFN-β promoters by MDA5. We selected the NS1 protein for further study and found that NS1 does not affect the interaction between the viral ribonucleoprotein (RNP) subunit and MDA5, but that it downregulates the expression of the laboratory of genetics and physiology 2 (LGP2) and retinoic acid-inducible gene-I (RIG-I) receptors in the RIG-I pathway. Additionally, NS1 was found to inhibit the expression of several antiviral proteins and cytokines, including MX dynamin like GTPase 1 (MX1), 2′-5′oligoadenylate synthetase (OAS), Signal Transducers and Activators of Transcription (STAT1), tripartite motif 25 (TRIM25), interleukin-2 (IL-2), IFN, IL-8, and IL-1β. To further investigate the role of NS1, a recombinant H3N2 virus strain (rH3N2) and an NS1-null virus (rH3N2ΔNS1) were rescued using reverse-genetic technology. The rH3N2ΔNS1 virus exhibited lower viral titers compared to rH3N2, but had a stronger activation effect on the receptors LGP2 and RIG-I. Furthermore, when compared to rH3N2, rH3N2ΔNS1 exhibited a more pronounced activation of antiviral proteins such as MX1, OAS, STAT1, and TRIM25, as well as antiviral cytokines such as IL-6, IFN-β, and IL-1β. These findings suggest a new mechanism by which NS1, a nonstructural protein of CIV, facilitates innate immune signaling and provides new avenues for the development of antiviral strategies.
A Single-Product Multi-Period Inventory Routing Problem under Intermittent Demand
Authors: Song Xin , Chang Daofang , Luo Tian
Published: June 2023

Demand fluctuations and uncertainty bring challenges to inventory management, and intermittent demand patterns increase the risk of inventory backlogs and raise inventory holding costs. In previous studies on inventory routing problems, different variants have been proposed to cope with complicated industrial scenarios. However, there are few studies on inventory routing problems with intermittent demand patterns. To solve this problem, we introduce a lateral transshipment strategy and build a single-product multi-period inventory routing mixed integer programming model to reduce customers’ inventory backlogs, balance regional inventory, reduce inventory holding costs, and improve inventory management efficiency. Furthermore, we design an adaptive large-neighborhood search algorithm with new operators to improve the solving efficiency. The experimental results show that an appropriate transshipment price can reduce the share of distribution costs. Another finding is that higher-capacity vehicles lead to higher revenue. Our findings not only expand the scope of the IRP domain but also provide actionable management insights for business practitioners.
NUMSnet: Nested-U Multi-Class Segmentation Network for 3D Medical Image Stacks
Authors: Roychowdhury Sohini
Published: June 2023

The semantic segmentation of 3D medical image stacks enables accurate volumetric reconstructions, computer-aided diagnostics and follow-up treatment planning. In this work, we present a novel variant of the Unet model, called the NUMSnet, that transmits pixel neighborhood features across scans through nested layers to achieve accurate multi-class semantic segmentation with minimal training data. We analyzed the semantic segmentation performance of the NUMSnet model in comparison with several Unet model variants in the segmentation of 3–7 regions of interest using only 5–10% of images for training per Lung-CT and Heart-CT volumetric image stack. The proposed NUMSnet model achieves up to 20% improvement in segmentation recall, with 2–9% improvement in Dice scores for Lung-CT stacks and 2.5–16% improvement in Dice scores for Heart-CT stacks when compared to the Unet++ model. The NUMSnet model needs to be trained with ordered images around the central scan of each volumetric stack. The propagation of image feature information from the six nested layers of the Unet++ model are found to have better computation and segmentation performance than the propagation of fewer hidden layers or all ten up-sampling layers in a Unet++ model. The NUMSnet model achieves comparable segmentation performance to previous works while being trained on as few as 5–10% of the images from 3D stacks. In addition, transfer learning allows faster convergence of the NUMSnet model for multi-class semantic segmentation from pathology in Lung-CT images to cardiac segmentation in Heart-CT stacks. Thus, the proposed model can standardize multi-class semantic segmentation for a variety of volumetric image stacks with a minimal training dataset. This can significantly reduce the cost, time and inter-observer variability associated with computer-aided detection and treatment.
Diversity and Dynamics of Bacterial Communities in the Digestive and Excretory Systems across the Life Cycle of Leafhopper, Recilia dorsalis
Authors: Huang Qiuyan , Shan Hong-Wei , Chen Jian-Ping , Wu Wei
Published: June 2023

Recilia dorsalis is a notorious rice pest that harbors numerous symbiotic microorganisms. However, the structure and dynamics of bacterial communities in various tissues of R. dorsalis throughout its life cycle remain unclear. In this study, we used high-throughput sequencing technology to analyze the bacterial communities in the digestive, excretory, and reproductive systems of R. dorsalis at different developmental stages. The results showed that the initial microbiota in R. dorsalis mostly originated from vertical transmission via the ovaries. After the second-instar nymphs, the diversity of bacterial communities in the salivary gland and Malpighian tubules gradually decreased, while the midgut remained stable. Principal coordinate analysis revealed that the structure of bacterial communities in R. dorsalis was primarily influenced by the developmental stage, with minimal variation in bacterial species among different tissues but significant variation in bacterial abundance. Tistrella was the most abundant bacterial genus in most developmental stages, followed by Pantoea. The core bacterial community in R. dorsalis continuously enriched throughout development and contributed primarily to food digestion and nutrient supply. Overall, our study enriches our knowledge of the bacterial community associated with R. dorsalis and provides clues for developing potential biological control technologies against this rice pest.
Honey Bee Colonies (Apis mellifera L.) Perform Orientation Defensiveness That Varies among Bred Lines
Authors: Akongte Peter Njukang , Park Bo-Sun , Kim Dong-Won , Choi Yong-Soo
Published: June 2023

Honey bees (Apis mellifera L.) express complex behavioral patterns (aggressiveness) in defensive mechanisms for their survival. Their phenotypic expression of defensive behavior is influenced by internal and external stimuli. Knowledge of this behavior has recently become increasingly important, though beekeepers are still faced with the challenges of selecting defensive and less-defensive bred lines. Field evaluation of defensive behavior among bred lines of honey bees is required to overcome the challenges. Chemical cues (alarm pheromone and isopentyl acetate mixed with paraffin oil) and physical and visual stimuli (dark leather suede, colony marbling, and suede jiggling) were used to evaluate defensiveness and orientation among five bred lines of honeybee colonies. Our results showed that both chemical assays recruited bees, but the time of recruitment was significantly faster for alarm pheromone. Honeybees’ response to both assays culminated in stings that differed among bred lines for alarm pheromone and paraffin when colonies were marbled. Honeybee orientation defensiveness varied among bred lines and was higher in more defensive bred lines compared to less-defensive bred lines. Our findings suggest that it is crucial to repeatedly evaluate orientation defensiveness at the colony level and among bred lines when selecting breeding colonies.
Nudging Consumer Behavior with Social Marketing in Portugal: Can Perception Have an Influence over Trying Insect-Based Food?
Authors: Bucea-Manea-Țoniș Rocsana , Martins Oliva M. D. , Urdeș Laura , Coelho Ana Sofia , Simion Violeta-Elena
Published: June 2023

Social marketing campaigns are widely used to inform, educate, communicate, and promote healthy behaviors that add benefits to the individual, but also to society and the environment. Considering the low cost and high quality of insect-based food, this research aims to identify the main factors which can be used by social marketing campaigns to help people to try new foods, such as insect-based food. Although it is considered an important alternative to protein, there are a few countries that have not experienced it. In many Western countries, insect-based food is perceived as being disgusting. Neophobia is also a barrier to trying these foods. The main goal is to analyze if social marketing campaigns might influence perception (familiarity, preparation, visual, and information). Our model proves this assumption because we obtained high path coefficients, indicating that perception influences social beliefs, individual beliefs, and consumption intention. Thus, they will increase the consumption intention.
Northern Richness, Southern Dead End—Origin and Dispersal Events of Pseudolycoriella (Sciaridae, Diptera) between New Zealand’s Main Islands
Authors: Köhler Arne , Schmitt Thomas
Published: June 2023

Sciaridae (Diptera) is a widespread insect family of which some species can reach high abundances in arboreal habitats. This trait, together with their (passive) mobility, enables them to quickly colonise suitable habitats. To reveal the biogeographic history of the New Zealand members of the sciarid genus Pseudolycoriella, we analysed three molecular markers of selected species and populations in a Bayesian approach. At the intra- and interspecific levels, we detected a pattern of northern richness vs. southern purity, which has probably developed as a result of Pleistocene glacial cycles. Since the late Miocene, we identified 13 dispersal events across the sea strait separating New Zealand’s main islands. As nine of these dispersal events were south-directed, North Island can be considered the centre of radiation for this genus. An unequivocal re-colonisation of North Island was only observed once. Based on the inclusion of three undescribed species from Tasmania and on previously published data, three colonisations of New Zealand are likely, all of them assumed to be of Australian origin. One of these most probably took place during the late Miocene, and the other two during the late Pliocene or at the Pliocene–Pleistocene boundary.
What’s New in Research during the Current Epidemic Wave of Eating Disorders?
Authors: Panero Matteo , Abbate-Daga Giovanni
Published: June 2023

The COVID-19 pandemic has caused physical health concerns and has significantly impacted mental health [...]
Metabolism of Acetaminophen by Enteric Epithelial Cells Mitigates Hepatocellular Toxicity In Vitro
Authors: Morgan Katie , Morley Steven D. , Raja Arslan K. , Vandeputte Martin , Samuel Kay , Waterfall Martin , Homer Natalie Z. M. , Hayes Peter C. , Fallowfield Jonathan A. , Plevris John N.
Published: June 2023

The gut–liver axis is defined by dietary and environmental communication between the gut, microbiome and the liver with its redox and immune systems, the overactivation of which can lead to hepatic injury. We used media preconditioning to mimic some aspects of the enterohepatic circulation by treating the human Caco-2 intestinal epithelial cell line with 5, 10 and 20 mM paracetamol (N-acetyl-para-aminophenol; APAP) for 24 h, after which cell culture supernatants were transferred to differentiated human hepatic HepaRG cells for a further 24 h. Cell viability was assessed by mitochondrial function and ATP production, while membrane integrity was monitored by cellular-based impedance. Metabolism by Caco-2 cells was determined by liquid chromatography with tandem mass spectrometry. Caco-2 cell viability was not affected by APAP, while cell membrane integrity and tight junctions were maintained and became tighter with increasing APAP concentrations, suggesting a reduction in the permeability of the intestinal epithelium. During 24 h incubation, Caco-2 cells metabolised 64–68% of APAP, leaving 32–36% of intact starting compound to be transferred to HepaRG cells. When cultured with Caco-2-preconditioned medium, HepaRG cells also showed no loss of cell viability or membrane integrity, completely in contrast to direct treatment with APAP, which resulted in a rapid loss of cell viability and membrane integrity and, ultimately, cell death. Thus, the pre-metabolism of APAP could mitigate previously observed hepatotoxicity to hepatic tight junctions caused by direct exposure to APAP. These observations could have important implications for the direct exposure of hepatic parenchyma to APAP, administered via the intravenous route.
Cycle Biodynamics of Women’s Microbiome in the Urinary and Reproductive Systems
Authors: Grobeisen-Duque Orly , Mora-Vargas Carlos Daniel , Aguilera-Arreola Ma. Guadalupe , Helguera-Repetto Addy Cecilia
Published: June 2023

The genitourinary microbiome plays a crucial role in the establishment and maintenance of urinary and reproductive health in women throughout their lives. Particularly during the reproductive stage, resident microorganisms contribute to implantation and protect against perinatal complications, including preterm birth, stillbirth, and low birth weight, while also serving as the first line of defense against pathogens that can cause infections, such as urinary tract infections and bacterial vaginosis. This review aimed to elucidate the relationship between a healthy microbiome environment and women’s overall health. We examine the variability and dynamics of the microbiome during different developmental stages, ranging from the prepubertal to the postmenopausal stage. Furthermore, we explore the significance of a healthy microbiota in successful implantation and pregnancy development and investigate potential differences between women experiencing infertility. In addition, we analyze the local and systemic inflammatory responses associated with the establishment of a dysbiotic state and compare it to a condition where a healthy microbiome was established. Lastly, we present the most recent evidence regarding preventive measures, such as dietary interventions and the use of probiotics to promote and maintain a healthy microbiome, thereby ensuring comprehensive women’s health. By highlighting the importance of the genitourinary microbiome in reproductive health, this review aimed to enhance this microbiome’s visibility and significance in the field.
Psychological Factors as Risk Contributors for Poor Hip Function after Periacetabular Osteotomy
Authors: Fischer Maximilian , Nonnenmacher Lars , Möller Alexander , Hofer André , Reichert Johannes , Matziolis Georg , Zimmerer Alexander , Wassilew Georgi
Published: June 2023

Psychologic comorbidities have been identified as risk factors for poor outcomes in orthopedic procedures, but their influence on the outcome of hip-preserving periacetabular osteotomy (PAO) remains uncertain. This retrospective cohort study aimed to assess the impact of patients’ psychological health on the outcome of PAO in patients with hip dysplasia (HD) and acetabular retroversion (AR). The study included 110 patients undergoing PAO for HD or AR between 2019 and 2021. Standardized questionnaires were administered to assess psychological factors, postoperative hip function, and activity level (mean follow-up: 25 months). Linear regression analyses were used to examine the associations between psychological factors and postoperative hip function and activity level. Both HD and AR patients showed improved postoperative hip function and activity levels. Linear regression analyses revealed that depression significantly impaired postoperative outcomes in both groups, whereas somatization negatively influenced the outcome in AR patients. General health perceptions significantly contributed to an improved postoperative outcome. These findings highlight the importance of concomitantly addressing psychologically relevant factors in order to improve patient outcomes after PAO procedures. Future prospective studies should continue to investigate the impact of various psychological factors and explore possibilities of incorporating psychological support into routine postoperative care for these patient cohorts.
Augmentation Therapies as Treatments for Coexisting Somatic Problems in Schizophrenia—A Systematic Review
Authors: Dróżdż Wiktor , Wiciński Michał , Szota Anna Maria , Szambelan Monika , Radajewska Izabela , Popławski Igor , Wojciechowski Paweł
Published: June 2023

The aim of this review is to appraise the data from available randomized clinical trials (RCT) regarding the possible combinations of neuroleptic and non-antipsychotic treatment which could enhance antipsychotic therapy efficacy whilst simultaneously addressing somatic symptoms in individuals with schizophrenia. A systematic search of the PubMed database up to February 2022 was conducted. Inclusion criteria: randomized controlled trials using augmentation therapy in chronic schizophrenia in adults, written in English, and only studies with psychometric assessments of schizophrenia were incorporated. Exclusion criteria: non-clinical, first episode of schizophrenia, patients on medication other than antipsychotics augmented, and not adjunctive therapy. Overall, 37 studies of 1931 patients with schizophrenia who received a combination of antipsychotic medication with other drugs were selected. A statistically significant reduction of negative and positive symptoms of schizophrenia, measured with the PANSS scale, when using a combination of antipsychotic treatment along with aspirin, simvastatin, N-acetylcysteine, or pioglitazone was found. A combination of antipsychotic medication with aspirin, simvastatin, N-acetylcysteine, or pioglitazone seems to be effective in the reduction of symptoms of schizophrenia in adults, but long-term studies are required to confirm this effect.