bluuebunny/crossref_metadata_2025 huggingface dataset:
Key characters uniting hemichordates and chordates: homologies or homoplasies?
Authors: Ruppert Edward E
Published: April 2005

Four chordate characters — dorsal hollow nerve cord, notochord, gill slits, and endostyle — are compared morphologically, molecularly, and functionally with similar structures in hemichordates to assess their putative homologies. The dorsal hollow nerve cord and enteropneust neurocord are probably homoplasies. The neurocord (= collar cord) may be an autapomorphy of Enteropneusta that innervates a unique pair of muscles, the perihemal coelomic muscles. Despite the apparent lack of organ-level homology, chordates and enteropneusts share a common pattern of neurulation that preserves a "contact innervation" between neuro- and myo-epithelia, which may be the primitive deuterostome pattern of neuromuscular innervation. The chordate notochord and hemichordate stomochord are probably homoplasies. Other potential notochord antecedents in hemichordates are examined, but no clear homolog is identified. The comparative morphology of notochords suggests that the "stack-of-coins" developmental stage, retained into adulthood only by cephalochordates, is the plesiomorphic notochord form. Hemichordate and chordate gill slits are probably homologs, but only at the level of simple ciliated circular or oval pores, lacking a skeleton, as occur in adults of Cephalodiscus spp., developmentally in some enteropneusts, and in many urochordates. Functional morphology, I125-binding experiments, and genetic data suggest that endostylar function may reside in the entire pharyngeal lining of Enteropneusta and is not restricted to a specialized midline structure as in chordates. A cladistic analysis of Deuterostomia, based partly on homologs discussed in this paper, indicates a sister-taxon relationship between Urochordata and Vertebrata, with Cephalochordata as the plesiomorphic clade.
A phylogeny of the hemichordates based on morphological characters
Authors: Cameron C B
Published: April 2005

A comprehensive review of literature on all 15 genera constituting the phylum Hemichordata resulted in a morphological matrix of 105 characters. The echinoderms, tunicates, cephalochordates, and vertebrates were included in the analysis, and the cnidarians, polychaetes, and sipunculids were employed as outgroup taxa. The consensus tree supported the traditional view of a monophyletic Hemichordata, Echinodermata, Ambulacraria, and Chordata. The enteropneust families Spengelidae and Ptychoderidae were each monophyletic and sister-taxa, but there was no resolution among the family Harrimaniidae. A detailed sensitivity analysis provided (i) tree lengths of competing evolutionary hypothesis and (ii) a test of monophyly of groups under a variety of evolutionary models. It is argued that the ancestral deuterostome was a benthic vermiform organism with a terminal mouth and anus, well-developed circular and longitudinal muscles, a simple nerve plexus with little sign of regionalization, a pharynx with gill slits and collagenous gill bars, a cluster of vacuolated cells with myofilaments, produced iodotyrosine, and displayed direct development. The pterobranchs have lost many of these features as a consequence of evolving a small body size and living in tubes, but these features exist in present-day enteropneusts, suggesting that they are a plausible model for the proximate ancestor of deuterostomes.
EXTREME FLUCTUATIONS IN SMALL-WORLD-COUPLED AUTONOMOUS SYSTEMS WITH RELAXATIONAL DYNAMICS
Authors: GUCLU H. , KORNISS G.
Published: April 2005

Synchronization is a fundamental problem in natural and artificial coupled multi-component systems. We investigate to what extent small-world couplings (extending the original local relaxational dynamics through the random links) lead to the suppression of extreme fluctuations in the synchronization landscape of such systems. In the absence of the random links, the steady-state landscape is "rough" (strongly de-synchronized state) and the average and the extreme height fluctuations diverge in the same power-law fashion with the system size (number of nodes). With small-world links present, the average size of the fluctuations becomes finite (synchronized state). For exponential-like noise the extreme heights diverge only logarithmically with the number of nodes, while for power-law noise they diverge in a power-law fashion. The statistics of the extreme heights are governed by the Fisher–Tippett–Gumbel and the Fréchet distribution, respectively. We illustrate our findings through an actual synchronization problem in parallel discrete-event simulations.
TRANSFER OF INFORMATION IN PARRONDO'S GAMES
Authors: AMENGUAL PAU , TORAL RAÚL
Published: April 2005

We study the relation between the discrete-time version of the flashing ratchet known as Parrondo's games and a compression technique used very recently with thermal ratchets for evaluating the transfer of information — negentropy — between the Brownian particle and the source of fluctuations. We present some results concerning different versions of Parrondo's games showing, for each case, a good qualitative agreement between the gain and the variation of the entropy.
VORTEX FLUCTUATIONS IN WEAK MAGNETIC FIELDS
Authors: SVEDLINDH PETER , FESTIN ÖRJAN , GUNNARSSON KLAS , RÖNNUNG FREDRIK , WINKLER DAG
Published: April 2005

Flux-noise and ac susceptibility measurements have been performed on epitaxial films of YBa 2 Cu 3 O 7. The validity of the fluctuation-dissipation-theorem is verified by the proportionality between flux noise and ac susceptibility results. The nominal zero-field flux-noise spectrum (Sϕ(f)) can be characterized as follows: Below a temperature dependent frequency f0(T), the flux-noise spectrum is frequency independent. For frequencies f>f0(T), the flux-noise spectrum follows f-x, with x≈1.7-1.8. f0 decreases by 4 orders of magnitude in a temperature interval of ΔT≈0.5 K . Moreover, the flux-noise spectrum scales as fSϕ=g(f/f0(T)), where g(·) is a scaling function. The influence of weak perturbing magnetic fields was investigated. While the general characteristics of the flux-noise spectrum remain, the characteristic frequency f0 increases by 3-4 orders of magnitude increasing the field from zero to 1 Oe. Flux-noise measurements performed using a compensating field to reduce the residual field in the experimental setup reveal new features of the measured noise spectra. The flux-noise is dominated by thermally generated vortex-antivortex pair fluctuations at temperatures below the mean-field transition temperature, while with decreasing temperature there is a transition to vortex fluctuations being dominated by field generated vortices.
ESTIMATION OF DETECTION LIMITS OF THE PHAGE-INVASION BASED IDENTIFICATION OF BACTERIA
Authors: KISH L. B. , CHENG M. , KIM J. U. , SEO S. , KING M. D. , YOUNG R. , DER A. , SCHMERA G.
Published: April 2005

Recently a new method, SEnsing of Phage-Triggered Ion Cascade (SEPTIC) was proposed for the rapid detection and identification of bacteria via the electrical field caused by the stochastic emission of ions during phage infection. In this Letter, we present linear network theoretical considerations about the detection limits of the method. The considerations are based on our published data of the E. coli detection experiments and on the assumption of a linear response between the number of bacteria and the measured power density spectrum of the fluctuation-signal. Some practical limits of the detectability of the present agents with possible noise measurement arrangements are discussed in this paper. The calculations indicate that the detection and identification of a single bacterium can be achieved with natural (wild) phages with reasonable efforts within a time window of 10 minutes.
EFFECTS OF SELECTIVE ADVANTAGE OF HbS OVER HbA IN THE TRANSMISSION DYNAMICS OF SICKLE CELL ANAEMIA
Authors: TCHUENCHE JEAN M.
Published: April 2005

Despite rising concern over the biology of Sickle Cell Anaemia (SCA), no general modeling approach has considered the effects of the selective advantage of Haemoglobin S (HbS) over Haemoglobin (HbA). This paper develops a theoretical framework and demonstrates mathematically that mating between the three different genotypic groups, namely: normal AA, carriers AS and sicklers SS, does not yield equal probabilities of having neonates of these classes.
EMPIRICAL DATA AND MATHEMATICAL STRUCTURES IN THE EPIDEMIC MODELING OF PARENTERAL HEPATITIS IN ITALY
Authors: SCHINAIA GIUSEPPE
Published: April 2005

Viral liver infections with parenteral transmission in Western countries are mostly caused by hepatitis B and hepatitis C viruses (HBV and HCV). This paper presents a mathematical model that describes the history of the spread of HBV and HCV infections in the general population in Italy. The analysis of the model and the results also provide some new insight into the mechanisms of the epidemics. The model structure is based on an underlying analysis of the various effects of the infection progression in the host, in order to incorporate into its parameters most of the information available from the literature. Moreover, incidence and prevalence curves of both HBV and HCV infections and of HBV/HCV co-infections are generated and qualitative aspects of the epidemic, such as possible endemic steady states and the basic reproduction number, are also analyzed.
HOW INDIVIDUALS LEARN TO TAKE TURNS: EMERGENCE OF ALTERNATING COOPERATION IN A CONGESTION GAME AND THE PRISONER'S DILEMMA
Authors: HELBING DIRK , SCHÖNHOF MARTIN , STARK HANS-ULRICH , HOŁYST JANUSZ A.
Published: April 2005

In many social dilemmas, individuals tend to generate a situation with low payoffs instead of a system optimum ("tragedy of the commons"). Is the routing of traffic a similar problem? In order to address this question, we present experimental results on humans playing a route choice game in a computer laboratory, which allow one to study decision behavior in repeated games beyond the Prisoner's Dilemma. We will focus on whether individuals manage to find a cooperative and fair solution compatible with the system-optimal road usage. We find that individuals tend towards a user equilibrium with equal travel times in the beginning. However, after many iterations, they often establish a coherent oscillatory behavior, as taking turns performs better than applying pure or mixed strategies. The resulting behavior is fair and compatible with system-optimal road usage. In spite of the complex dynamics leading to coordinated oscillations, we have identified mathematical relationships quantifying the observed transition process. Our main experimental discoveries for 2- and 4-person games can be explained with a novel reinforcement learning model for an arbitrary number of persons, which is based on past experience and trial-and-error behavior. Gains in the average payoff seem to be an important driving force for the innovation of time-dependent response patterns, i.e. the evolution of more complex strategies. Our findings are relevant for decision support systems and routing in traffic or data networks.
Cd2+-induced swelling-contraction dynamics in isolated kidney cortex mitochondria: role of Ca2+ uniporter, K+ cycling, and protonmotive force
Authors: Lee Wing-Kee , Spielmann Malte , Bork Ulrich , Thévenod Frank
Published: April 2005

The nephrotoxic metal Cd2+ causes mitochondrial damage and apoptosis of kidney proximal tubule cells. A K+ cycle involving a K+ uniporter and a K+/H+ exchanger in the inner mitochondrial membrane (IMM) is thought to contribute to the maintenance of the structural and functional integrity of mitochondria. In the present study, we have investigated the effect of Cd2+ on K+ cycling in rat kidney cortex mitochondria. Cd2+ (EC50 ∼19 μM) induced swelling of nonenergized mitochondria suspended in isotonic salt solutions according to the sequence KCl = NaCl > LiCl ≫ choline chloride. Cd2+-induced swelling of energized mitochondria had a similar EC50 value and showed the same cation dependence but was followed by a spontaneous contraction. Mitochondrial Ca2+ uniporter (MCU) blockers, but not permeability transition pore inhibitors, abolished swelling, suggesting the need for Cd2+ influx through the MCU for swelling to occur. Complete loss of mitochondrial membrane potential (ΔΨm) induced by K+ influx did not prevent contraction, but addition of the K+/H+ exchanger blocker, quinine (1 mM), or the electroneutral protonophore nigericin (0.4 μM), abolished contraction, suggesting the mitochondrial pH gradient (ΔpHm) driving contraction. Accordingly, a quinine-sensitive partial dissipation of ΔpHm was coincident with the swelling-contraction phase. The data indicate that Cd2+ enters the matrix through the MCU to activate a K+ cycle. Initial K+ load via a Cd2+-activated K+ uniporter in the IMM causes osmotic swelling and breakdown of ΔΨm and triggers quinine-sensitive K+/H+ exchange and contraction. Thus Cd2+-induced activation of a K+ cycle contributes to the dissipation of the mitochondrial protonmotive force.
NKCC1 and NHE1 are abundantly expressed in the basolateral plasma membrane of secretory coil cells in rat, mouse, and human sweat glands
Authors: Nejsum Lene N. , Praetorius Jeppe , Nielsen Søren
Published: April 2005

In isolated sweat glands, bumetanide inhibits sweat secretion. The mRNA encoding bumetanide-sensitive Na+-K+-Cl− cotransporter (NKCC) isoform 1 (NKCC1) has been detected in sweat glands; however, the cellular and subcellular protein localization is unknown. Na+/H+ exchanger (NHE) isoform 1 (NHE1) protein has been localized to both the duct and secretory coil of human sweat duct; however, the NHE1 abundance in the duct was not compared with that in the secretory coil. The aim of this study was to test whether mRNA encoding NKCC1, NKCC2, and Na+-coupled acid-base transporters and the corresponding proteins are expressed in rodent sweat glands and, if expressed, to determine the cellular and subcellular localization in rat, mouse, and human eccrine sweat glands. NKCC1 mRNA was demonstrated in rat palmar tissue, including sweat glands, using RT-PCR, whereas NKCC2 mRNA was absent. Also, NHE1 mRNA was demonstrated in rat palmar tissue, whereas NHE2, NHE3, NHE4, electrogenic Na+-HCO3− cotransporter 1 NBCe1, NBCe2, electroneutral Na+-HCO3− cotransporter NBCn1, and Na+-dependent Cl−/HCO3− exchanger NCBE mRNA were not detected. The expression of NKCC1 and NHE1 proteins was confirmed in rat palmar skin by immunoblotting, whereas NKCC2, NHE2, and NHE3 proteins were not detected. Immunohistochemistry was performed using sections from rat, mouse, and human palmar tissue. Immunoperoxidase labeling revealed abundant expression of NKCC1 and NHE1 in the basolateral domain of secretory coils of rat, mouse, and human sweat glands and low expression was found in the coiled part of the ducts. In contrast, NKCC1 and NHE1 labeling was absent from rat, mouse, and human epidermis. Immunoelectron microscopy demonstrated abundant NKCC1 and NHE1 labeling of the basolateral plasma membrane of mouse sweat glands, with no labeling of the apical plasma membranes or intracellular structures. The basolateral NKCC1 of the secretory coils of sweat glands would most likely account for the observed bumetanide-sensitive NaCl secretion in the secretory coils, and the basolateral NHE1 is likely to be involved in Na+-coupled acid-base transport.
Desensitization of canonical transient receptor potential channel 5 by protein kinase C
Authors: Zhu Mei Hong , Chae MeeRee , Kim Hyun Jin , Lee Young Mee , Kim Min Ji , Jin Nan Ge , Yang Dong Ki , So Insuk , Kim Ki Whan
Published: April 2005

The classic type of transient receptor potential channel (TRPC) is a molecular candidate for Ca2+-permeable cation channel in mammalian cells. TRPC5 is desensitized rapidly after activation by G protein-coupled receptor. Herein we report our investigation into the desensitization of mTRPC5 and localization of the molecular determinants of this desensitization using mutagenesis. TRPC5 was initially activated by muscarinic stimulation using 100 μM carbachol (CCh) and then decayed rapidly even in the presence of CCh (desensitization). Increased EGTA or omission of MgATP in the pipette solution slowed the rate of this desensitization. The protein kinase C (PKC) inhibitors, 1 μM chelerythrine, 100 nM GF109203X, or PKC peptide inhibitor (19–36), inhibited this desensitization of TRPC5 activated by 100 μM CCh. When TRPC5 current was activated by intracellular GTPγS, PKC inhibitors prevented TRPC5 desensitization and the mutation of TRPC5 T972 to alanine slowed the desensitization process dramatically. We conclude that the desensitization of TRPC5 occurs via PKC phosphorylation and suggest that threonine at residue 972 of mouse TRPC5 might be required for its phosphorylation by PKC.
Cross-Orientation Suppression: Monoptic and Dichoptic Mechanisms Are Different
Authors: Li Baowang , Peterson Matthew R. , Thompson Jeffrey K. , Duong Thang , Freeman Ralph D.
Published: April 2005

The response of a cell in the primary visual cortex to an optimally oriented grating is suppressed by a superimposed orthogonal grating. This cross-orientation suppression (COS) is exhibited when the orthogonal and optimal stimuli are presented to the same eye (monoptically) or to different eyes (dichoptically). A recent study suggested that monoptic COS arises from subcortical processes; however, the mechanisms underlying dichoptic COS were not addressed. We have compared the temporal frequency tuning and stimulus adaptation properties of monoptic and dichoptic COS. We found that dichoptic COS is best elicited with lower temporal frequencies and is substantially reduced after prolonged adaptation to a mask grating. In contrast, monoptic COS is more pronounced with mask gratings at much higher temporal frequencies and is less prone to stimulus adaptation. These results suggest that monoptic COS is mediated by subcortical mechanisms, whereas intracortical inhibition is the mechanism for dichoptic COS.
Long-Latency Afferent Inhibition During Selective Finger Movement
Authors: Voller Bernhard , St Clair Gibson Alan , Lomarev Mikhail , Kanchana Sulada , Dambrosia James , Dang Nguyet , Hallett Mark
Published: April 2005

Stimulation of a peripheral nerve of a hand at rest modulates excitability in the motor cortex and, in particular, leads to inhibition when applied at an interval of ∼200 ms (long-latency afferent inhibition; LAI). Surround inhibition (SI) is the process that inhibits neighboring muscles not involved in a particular task. The neuronal mechanisms of SI are not known, and it is possible that LAI might contribute to it. Using transcranial magnetic stimulation (TMS) with and without movement of the index finger, the motor-evoked potentials (MEPs) were measured of two functionally distinct target muscles of the hand (abductor digiti minimi muscle = ADM, 1st dorsal interosseus muscle = FDI). Electrical stimulation was applied 180 ms before TMS to either the fifth finger or the index finger. Both homotopic and heterotopic finger stimulation resulted in LAI without movement. With index finger movement, motor output further decreased with homo- and heterotopic stimulation in the ADM. In the moving FDI, however, there was no change with either homo- or heterotopic stimulation. Additionally, in the unstimulated movement trials, LAI increased with the amount of unintentional co-activation that occurred despite attempts to maintain the ADM at rest. However, with finger stimulation added, there were almost no increased MEPs despite co-activation. These findings suggest that LAI increases during movement and can enhance SI.
Fimbria-Fornix Lesions Compromise the Induction of Long-Term Potentiation at the Schaffer Collateral-CA1 Synapse in the Rat In Vivo
Authors: Li Chaoying , Maier Donna L. , Cross Ben , Doherty James J. , Christian Edward P.
Published: April 2005

Although bilateral fimbria-fornix (FF) lesioning impairs spatial performance in animals, the literature is equivocal regarding its effects on hippocampal long-term potentiation (LTP). We examined the effects of FF lesioning on LTP induction in the Schaffer collateral–CA1 pathway in vivo with a protocol that delivered theta burst stimulation (TBS) trains of increasing length until a sufficient length was reached to induce LTP of the monosynaptic field excitatory postsynaptic potential (fEPSP). Experiments were performed in urethan-anesthetized Long-Evans rats either 4 or 12–16 wk after lesioning. In sham-operated controls, TBS trains ranging from 4 to 12 bursts were sufficient to induce robust LTP [170 ± 10% (mean ± SF) of control fEPSP slope; n = 8]. Four-week post -FF-lesioned animals also displayed clear LTP (167 ± 12% of control fEPSP slope; n = 4) that did not differ from the shams ( P > 0.05). In contrast, animals in the 12- to 16-wk post-lesion group showed a highly significant deficit in LTP induction (95 ± 3% of control fEPSP slope; n = 8; ≤28 burst TBS trains tested; P < 0.001 vs. sham- and 4-wk post-FF-lesion groups). Other quantitative measures of synaptic excitability (i.e., baseline fEPSP slope and input-output relation) did not differ between the sham- and the 12- to 16-wk post-FF-lesion groups. These results indicate that the FF lesion leads to an enduring defect in hippocampal long-term synaptic plasticity that may relate mechanistically to the cognitive deficits characterized in this model.
Temporomandibular Joint Inflammation Potentiates the Excitability of Trigeminal Root Ganglion Neurons Innervating the Facial Skin in Rats
Authors: Takeda Mamoru , Tanimoto Takeshi , Ikeda Mizuho , Nasu Masanori , Kadoi Jun , Shima Yukio , Ohta Hidehiko , Matsumoto Shigeji
Published: April 2005

The aim of this study was to test the hypothesis that temporomandibular joint (TMJ) inflammation alters the excitability of trigeminal root ganglion (TRG) neurons innervating the facial skin, by using behavioral, electrophysiological, molecular, and immunohistochemical approaches. Complete Freund’s adjuvant (CFA) was injected into the rat TMJ to produce inflammation. The threshold for escape from mechanical stimulation applied to the orofacial area in TMJ-inflamed rats was significantly lower than that in naïve rats. The TRG neurons innervating the inflamed TMJ were labeled by 2% Fluorogold (FG) injection into the TMJ. The number of FG-labeled substance P (SP)-immunoreactive neurons in the inflamed rats was significantly increased compared with that in the naïve rats. On the other hand, medium- and large-diameter TRG neurons (>30 μm) innervating the facial skin were labeled by FG injection into the facial skin. In the FG-labeled cutaneous TRG neurons, the occurrence of SP (100 nM) induced membrane depolarization in inflamed rats (medium: 73.3%, large : 85.7%) was larger than that in the naïve rats (medium: 29.4%, large : 0%). In addition, SP application significantly increased the firing rate evoked by depolarizing pulses in the neurons of inflamed rats compared with those of naïve rats. Quantitative single-cell RT-PCR analysis showed the increased expression of mRNA for the NK1 receptor in FG-labeled TRG neurons in inflamed rats compared with that in naïve rats. The numbers of SP and NK1 receptors/neurofilament 200 positive immunoreactive TRG neurons innervating the facial skin (FG-labeled) in the inflamed rats were significantly increased compared with those seen in naïve rats. These results suggest that TMJ inflammation can alter the excitability of medium- and large-diameter TRG neurons innervating the facial skin and that an increase in SP/NK1 receptors in their soma may contribute to the mechanism underlying the trigeminal inflammatory allodynia in the TMJ disorder.
Properties of Short-Term Synaptic Depression at Larval Neuromuscular Synapses in Wild-Type and Temperature-Sensitive Paralytic Mutants ofDrosophila
Authors: Wu Ying , Kawasaki Fumiko , Ordway Richard W.
Published: April 2005

The larval neuromuscular synapse of Drosophila serves as an important model for genetic and molecular analysis of synaptic development and function. Further functional characterization of this synapse, as well as adult neuromuscular synapses, will greatly enhance the impact of this model system on our understanding of synaptic transmission. Here we describe a form of short-term synaptic depression observed at larval, but not adult, neuromuscular synapses and explore the underlying mechanisms. Larval neuromuscular synapses exhibited a form of short-term depression that was strongly dependent on stimulation frequency over a narrow range of low frequencies (0.1–1 Hz). This form of synaptic depression, referred to here as low-frequency short-term depression (LF-STD), results from an activity-dependent reduction in neurotransmitter release. However, in contrast to the predictions of depletion models, the degree of depression was independent of the initial level of neurotransmitter release over a range of extracellular calcium concentrations. This conclusion was confirmed in two temperature-sensitive (TS) paralytic mutants, cacophony and shibire, which exhibit reduced neurotransmitter release resulting from conditional disruption of presynaptic calcium channels and dynamin, respectively. Higher stimulation frequencies (40 or 60 Hz) produced two components of depression that appeared to include LF-STD as well as a more conventional component of short-term depression. These findings reveal novel properties of short-term synaptic depression and suggest that complementary genetic analysis of larval and adult neuromuscular synapses will further define the in vivo mechanisms of neurotransmitter release and short-term synaptic plasticity.
Constructing Coercion
Authors: Monahan Brian A. , Marolla Joseph A. , Bromley David G.
Published: April 2005

There is an abundance of research on how perpetrators organize and orchestrate their activity during the commission of burglary, robbery, and homicide. By contrast, there is very little research on how perpetrators organize sexual assaults. Based on interviews with 33 incarcerated rapists who acted alone and had little or no prior social connection to their victims, we describe rape events in terms of a sequential series of phases that are analogous to those employed to analyze homicides and robbery. The five phases of the kind of rape events we describe include (1) preexisting life tensions, (2) transformation of motivation into action, (3) perpetrator-victim confrontation, (4) situation management, and (5) disengagement. We also argue that within these five phases, perpetrators exhibit differential awareness of their own actions, apply divergent meanings to apparently similar actions, and engage in different degrees and types of organization.
Not Just a Paper Doll
Authors: Mears Ashley , Finlay William
Published: April 2005

Modeling is a challenging occupation because employment is irregular, the physical demands are great, and competition is fierce. Success as a model requires the careful management of bodily capital and the performance of emotional labor. Drawing on participant observations and interviews with models in the Atlanta fashion industry, the authors examine how they do the former and why they do the latter. They manage their bodily capital by subjecting themselves to intense self-regulation. Models perform emotional labor to sell themselves to clients and agents, to create illusions for observers and the camera, and to find dignity in a job that is often degrading and humiliating.
Clinically diagnosed childhood asthma and follow-up of symptoms in a Swedish case control study
Authors: Roel Eduardo , Faresjö Åshild , Zetterström Olle , Trell Erik , Faresjö Tomas
Published: April 2005

Background

Childhood asthma has risen dramatically not only in the western societies and now forms a major and still increasing public health problem. The aims of this study were to follow up at the age of ten the patterns of asthma symptoms and associations among children with a clinically diagnosed asthma in a sizeable urban-rural community and to in compare them with demographic controls using a standardised questionnaire.

Methods

In a defined region in Sweden with a population of about 150 000 inhabitants, all children (n = 2 104) born in 1990 were recorded. At the age of seven all primary care and hospital records of the 1 752 children still living in the community were examined, and a group of children (n = 191) was defined with a well-documented and medically confirmed asthma diagnosis. At the age of ten, 86 % of these cases (n = 158) and controls (n = 171) completed an ISAAC questionnaire concerning asthma history, symptoms and related conditions.

Results

Different types of asthma symptoms were highly and significantly over-represented in the cases. Reported asthma heredity was significantly higher among the cases. No significant difference in reported allergic rhinitis or eczema as a child was found between cases and controls. No significant difference concerning social factors or environmental exposure was found between case and controls. Among the control group 4.7 % of the parents reported that their child actually had asthma. These are likely to be new asthma cases between the age of seven and ten and give an estimated asthma prevalence rate at the age of ten of 15.1 % in the studied cohort.

Conclusion

A combination of medical verified asthma diagnosis through medical records and the use of self-reported symptom through the ISAAC questionnaire seem to be valid and reliable measures to follow-up childhood asthma in the local community. The asthma prevalence at the age of ten in the studied birth cohort is considerably higher than previous reports for Sweden. Both the high prevalence figure and allowing the three-year lag phase for further settling of events in the community point at the complementary roles of both hospital and primary care in the comprehensive coverage and control of childhood asthma in the community.
Compulsory and recommended vaccination in Italy: evaluation of coverage and non-compliance between 1998-2002 in Northern Italy
Authors: Stampi Serena , Ricci Rita , Ruffilli Isa , Zanetti Franca
Published: April 2005

Background

Since vaccinations are an effective prevention tool for maintaining the health of society, the monitoring of immunization coverage allows us to identify areas where disease outbreaks are likely to occur, and possibly assist us in predicting future outbreaks. The aim of this study is the investigation of the coverage achieved for compulsory (diphtheria, tetanus, polio, hepatitis B,) and recommended (pertussis, Haemophilus influenzae, measles-mumps-rubella) vaccinations between 1998 and 2002 in the municipality of Bologna and the identification of the subjects not complying with compulsory and recommended vaccinations.

Methods

The statistics regarding vaccinal coverage were elaborated from the data supplied by the Bologna vaccinal registration system (1998–2000) and the IPV4 program (2001–2002). To calculate the coverage for compulsory vaccinations and cases of non-compliance reference was made to the protocol drawn up by the Emilia Romagna Regional Administration. The reasons for non-compliance were divided into various categories

Results

In Bologna the levels of immunization for the four compulsory vaccinations are satisfactory: over 95% children completed the vaccinal cycle, receiving the booster for anti-polio foreseen in their 3rd year and for anti-dyphteria, tetanus, pertussis at 6 years. The frequency of subjects with total non-compliance (i.e. those who have not begun any compulsory vaccinations by the age of one year) is generally higher in Bologna than in the region, with a slight increase in 2002 (2.52% and 1.06% in the city and the region respectively). The frequency of the anti-measles vaccination is higher than that of mumps and rubella, which means that the single vaccine, as opposed to the combined MMR (measles-mumps-rubella) was still being used in the period in question.

The most common reason for non compliance is objection of parents and is probably due to reduction of certain diseases or anxiety about the possible risks.

Conclusion

In Bologna the frequency of children aged 12 and 24 months who have achieved compulsory vaccination varied, in 2002, between 95% and 98%. As regards recommended vaccinations the percentage of coverage against Haemophilus influenzae is 93.3%, while the levels for measles, mumps and pertussis range from 84% to approx. 92%. Although these percentages are higher if compared to those obtained by other Italian regions, every effort should be made to strengthen the aspects that lead to a successful vaccinal strategy.
Relationship of patients' age to histopathological features of breast tumours in BRCA1 and BRCA2and mutation-negative breast cancer families
Authors: Eerola Hannaleena , Heikkilä Päivi , Tamminen Anitta , Aittomäki Kristiina , Blomqvist Carl , Nevanlinna Heli
Published: April 2005

Introduction

Our aim was to evaluate the relationship of patients' age to histopathological features of hereditary breast tumours in a series of breast cancer families not selected for age at diagnosis. In sporadic breast cancer, tumours from premenopausal patients have been shown to differ from those of postmenopausal patients, but this phenomenon has been little studied among familial patients.

Methods

Representative areas of all available breast cancer tissue specimens (n = 262) from 25 BRCA1, 20 BRCA2, and 74 non-BRCA1/2 breast cancer families were punched into a tissue microarray. Immunohistochemical staining of oestrogen receptor, progesterone receptor, ERBB2, and p53 as well as the histology and grade of tumours in these three groups of families were studied in different age groups and compared with each other.

Results

We found that only breast cancers from young (<50 years) BRCA1+ patients represent features documented as being typical of BRCA1-associated cancers, such as high tumour grade, negativity for oestrogen and progesterone receptors, and overexpression of p53. Among the BRCA2 families, the opposite was found, with a significantly higher frequency of tumours negative for oestrogen and progesterone receptors among the older patients than among the other groups, but no distinctive tumour characteristics among the younger BRCA2 patients.

Conclusion

Tumours of BRCA1 and BRCA2 carriers aged 50 years or more differed significantly from those of younger carriers. This difference may reflect different biological behaviour and pathways of tumour development among the older and the younger BRCA1 and BRCA2 patients, with impact also on prognosis and survival.
Percutaneous absorption and skin irritation upon low-level prolonged dermal exposure to nonane, dodecane and tetradecane in hairless rats
Authors: Babu RJ , Chatterjee A , Ahaghotu E , Singh M
Published: April 2005

Even though the dermal toxicity of hydrocarbon fuels has been well established in the literature, there is little information available on the dermal penetration kinetics and irritation potential of the individual hydrocarbons. The penetration and skin retention of nonane, dodecane and tetradecane was assessed in vitro using hairless rats’ skin. The effects of unocclusive dermal exposures of these chemicals (15 mL every 2 h for 8 h a day for four days) on the transepidermal water loss (TEWL) and erythema were measured in CD hairless rats. The expression of interleukin 1a (IL-1a) and TNF-a in the skin and blood were measured at the end of dermal exposures. The flux of dodecane was 3- and 77-fold higher than nonane and tetradecane. The retention of chemicals in stratum corneum (SC) was in the order of tetradecane-dodecane-nonane, and directly correlated to the log Kp (r2-0.9900) and molecular weight of the chemicals (r2-0.8782). The TEWL and erythema data indicate that irritation was in the following order: tetradecane-dodecane-nonane. Likewise, the expression of IL-1a in the blood and TNF-a in the skin after dermal exposures was higher for tetradecane followed by dodecane and nonane compared to control. In conclusion, the aliphatic hydrocarbon chemicals of the present study induced cumulative irritation upon low-level repeat exposures for a four-day period. The affinity of the chemicals to SC and their gradual accumulation in the skin in the present study is the probable cause for the differences in the skin irritation profiles of different aliphatic chemicals. The findings of the present study will be helpful in understanding the skin irritation response of the chemicals in humans; indeed the reality check arises from dermal exposures in humans and human experience in occupational handling of these chemicals.
Human health risk assessment of 2-mercaptobenzothiazole in drinking water
Authors: Whittaker Margaret H , Gebhart Ann Marie , Miller Thea Clipson , Hammer Frank
Published: April 2005

2-Mercaptobenzothiazole (MBT) is used as a vulcanization accelerator in rubber products that come into contact with potable drinking water. When such products are evaluated for contact with potable water and submitted for ANSI/NSF Standard 61 certification, any chemical extracting from these products must be below an appropriate action level of exposure. As defined by Standard 61, a total allowable concentration (TAC) is the maximum concentration of a nonregulated contaminant allowed in a public drinking water supply, and the single product allowable concentration (SPAC) is 10% of the TAC. Currently, MBT has a TAC of 40 mg/L and a SPAC of 4 mg/L. A comprehensive health effects evaluation of MBT was performed to determine whether these action levels should be revised. Epidemiological investigations indicate that workers occupationally exposed to MBT have an increased risk of death from bladder cancer. Genotoxicity investigations in bacterial and mammalian test systems provide some evidence indicating that MBT has the potential to induce mutations and chromosomal aberrations. Toxicity studies in rats and mice chronically exposed to MBT identified increases in various tumours, such as adrenal gland tumours, pituitary gland tumours, liver tumours and renal pelvis tumours. The biological significance of most of these tumours is questionable due to a variety of factors, such as a lack of dose-response between tumour incidence and dose, and the effect of test article vehicle (corn oil) upon tumour rates. Potential human health effects of exposure to MBT can be predicted from an NTP 2-year cancer study in rats, as well as epidemiological investigations in occupationally exposed workers. A comprehensive review of the epidemiological and toxicological dataset for MBT indicates that the induction of renal pelvis transitional cell tumours is the most sensitive and relevant health effects endpoint upon which to base a revised TAC and SPAC. A multistage model was used to extrapolate to low-dose MBT exposures due to the genotoxicity and clastogenicity of MBT. A TAC of 600 mg/L was derived for MBT, and was based upon an LED10 of 157.681 mg/kg per day. A SPAC of 60 mg/L was derived by multiplying the revised TAC by 0.10, and rounding to one significant figure. These revised action levels are based upon the most sensitive health effects endpoint, as well as current cancer risk assessment methodology.
Urinary nickel: measurement of exposure by inductively coupled plasma argon emission spectrometry
Authors: Koizumi Chisato , Usuda Kan , Hayashi Satsuki , Dote Tomotaro , Kono Koichi
Published: April 2005

Nickel is a rare earth metal and is widely used in modern industry. Its overexposure in human beings can provoke significant effects including lung, cardiovascular and kidney diseases. As an index of occupational exposure, urine is widely used for the monitoring of nickel concentration because it is a minimally invasive method. Recent studies have used atomic absorption spectrometry to measure nickel concentration. In this study, we introduced novel inductively coupled plasma argon emission spectrometry (ICPAES) which enables us to measure multiple elements simultaneously with smaller volume and with lower detection limits compared to conventional atomic absorption emission spectrometry, and we established the new measuring method by determining the appropriate wavelengths for nickel concentration. Furthermore, using the established new measuring method, we investigated the correlation between a single oral administration of nickel and urine elimination in rats. As a result, different concentrations of nickel standard solutions were measured by ICPAES, and among five specific wavelengths of nickel, 221.647 and 231.604 nm were chosen because they had the highest inclines of both signal to background ratio and emission intensity in simple linear regression analysis. Next, by using healthy human urine samples that had not been exposed to nickel, 231.604 nm was determined to be the most appropriate wavelength because it did not present abnormal intensity due to obstacle wavelength. Male Wistar rats received an oral administration of nickel ranging from 0.025 to 250 mg/kg, which is equivalent to 0.0015-15% of LD50, and during the following 24 h, urine samples were collected and the nickel concentration was measured by ICPAES. With a single oral administration of nickel, there was an increase in urine nickel concentration in a dose-dependent manner and the appropriate equation was developed. Acute renal failure was not observed in this dosage of oral nickel administration by analysing NAG, b2-microglobulin, urine albumin and urine protein. It was concluded that the obtained nickel reference values using ICPAES would be useful for the early diagnosis of nickel intoxication and in the assessment of the exposure to nickel.
Effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) on iron absorption in rats
Authors: Akdogan Mehmet , Gultekin Fatih , Yontem Mustafa
Published: April 2005

Aim: The effect of Mentha piperita (Labiatae) and Mentha spicata (Labiatae) teas, which contain different phenol compounds, on iron metabolism was studied. These teas grow in different areas of the city of Isparta, Turkey. These herbals were given to the rats in tea. Methods: Forty-eight male Wistar albino rats weighing 200-250 g were used for this study. The rats were divided into four groups of 12 animals: Group I received no herbal tea (control group); Group II received 20 g/L M. piperita tea; Group III received 20 g/L M. spicata tea; Group IV received 40 g/L M. spicata tea. Herbal teas were prepared daily and provided at all times to the rats over 30 days as drinking water. Results: M. piperita tea caused a decrease in serum iron and ferritin levels P <0.05), and caused an increase in unsaturated iron-binding capacity (UIBC) P <0.01). M. spicata tea caused no significant change in serum iron, ferritin levels and UIBC P <0.05). Conclusion: Both herbal teas inhibited Fe absorption. Inhibition caused by M. spicata tea was dose dependent. Therefore, when drinking these teas, their effect should be considered, especially for children and anemic patients.
Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue
Authors: Terzi Alpaslan , Iraz Mustafa , Sahin Semsettin , Ilhan Atilla , Idiz Nuri , Fadillioglu Ersin
Published: April 2005

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.
Development of the mammalian urethra is controlled by Fgfr2-IIIb
Authors: Petiot Anita , Perriton Claire L. , Dickson Clive , Cohn Martin J.
Published: April 2005

Development of external genitalia in mammalian embryos requires tight coordination of a complex series of morphogenetic events involving outgrowth,proximodistal and dorsoventral patterning, and epithelial tubulogenesis. Hypospadias is a congenital defect of the external genitalia that results from failure of urethral tube closure. Although this is the second most common birth defect in humans, affecting one in every 250 children, the molecular mechanisms that regulate morphogenesis of the mammalian urethra are poorly understood. We report that mice lacking the IIIb isoform of fibroblast growth factor receptor 2 (Fgfr2) exhibit severe hypospadias. Urethral signaling regions, as indicated by Shh and Fgf8 expression, are established in Fgfr2-IIIb null mice; however, cell proliferation arrests prematurely and maturation of the urethral epithelium is disrupted. Fgfr2-IIIb-/- mutants fail to maintain the progenitor cell population required for uroepithelial renewal during tubular morphogenesis. In addition, we show that antagonism of the androgen receptor (AR) leads to loss of Fgfr2-IIIb and Fgf10 expression in the urethra, and an associated hypospadias phenotype, suggesting that these genes are downstream targets of AR during external genital development. Genitourinary defects resulting from disruption of AR activity, by either genetic or environmental factors, may therefore involve negative regulation of the Fgfr2 pathway. This represents the first example of how the developing genitourinary system integrates cues from systemically circulating steroid hormones with a locally expressed growth factor pathway.