bluuebunny/crossref_metadata_2025 huggingface dataset:
The Allergen-Specific IgE Concentration Is Important for Optimal Histamine Release From Passively Sensitized Basophils
Authors: Stoffersen Peter , Skov Per S. , Poulsen Lars K. , Jensen Bettina M.
Published: April 2022

Background

The basophil histamine release (HR) assay can be used for allergy diagnosis in addition to the conventional measurement of allergen-specific IgE (sIgE). Passive sensitization of basophils increases the versatility and allows testing the biological relevance of allergen-induced IgE cross-linking in any serum unbiased by the cellular component. However, not all the patient sera perform equally well and we hypothesized that the absolute level and fraction of sIgE affect the performance. Choosing birch pollen allergy as a model, we investigated the concentration of sIgE needed for successful passive sensitization using soluble- or matrix-fixed Bet v 1.

Methods

Twenty-eight sera with Bet v 1 sIgE [7 sera within each allergy class (1: 0.1–0.70 kUA/L, 2: 0.71–3.50 kUA/L, 3: 3.51–17.50 kUA/L, and 4+: >17.50 kUA/L)] and a negative control serum pool were used to passively sensitize donor basophils, obtained from buffy coat blood (n= 3). The cells were incubated (30 min) with a soluble allergen (rBet v 1 from 0.2 to 50 ng/ml), matrix-fixed allergen (ImmunoCAP™ containing recombinant Bet v 1), or phorbol 12-myristate 13-acetate (PMA)/ionomycin mixture (maximal HR) and released histamine was quantified fluorometrically.

Results

The lowest level of Bet v 1 sIgE generating a detectable HR (HR > 10% of maximal release) in all the 3 runs was found to be 1.25 kUA/L (corresponding to allergy class 2, 0.71–3.50 kUA/L). Furthermore, sera from allergy classes 3 and 4+ ascertained a significant reproducible HR: 42/42 vs. 5/21 in allergy class 1 and 15/21 in allergy class 2. Using ImmunoCAP™s containing Bet v 1 as a matrix-fixed allergen system, similar results were obtained where the lowest sIgE concentration mediating an HR was 1.68 kUA/L and 7/7 for both allergy classes 3 and 4+.

Conclusion

The results demonstrate that the IgE titer is strikingly robust in predicting the ability to sensitize basophils and produce a measurable HR.
Silica Nanoparticle Acute Toxicity on Male Rattus norvegicus Domestica: Ethological Behavior, Hematological Disorders, Biochemical Analyses, Hepato-Renal Function, and Antioxidant-Immune Response
Authors: Almanaa Taghreed N. , Aref Mohamed , Kakakhel Mian Adnan , Elshopakey Gehad E. , Mahboub Heba H. , Abdelazim Aaser Mohamed , Kamel Samar , Belali Tareg M. , Abomughaid Mosleh M. , Alhujaily Muhanad , Fahmy Esraa M. , Ezzat Assayed Mohamed , Mostafa-Hedeab Gomaa , Daoush Walid M.
Published: April 2022

With extensive production and various applications of silica nanoparticles (SiNPs), there is a controversy regarding the ecotoxicological impacts of SiNPs. Therefore, the current study was aimed to assess the acute toxicity of silica nanoparticles in male Rattus norvegicus domestica after 24 and 96 h. Hematological, serum biochemical, stress biomarker, and immune-antioxidant parameters were addressed. Chemical composition, crystal structure, and the particle shape and morphology of SiNPs were investigated using XRD, FTIR, BET, UV-Vis, and SEM, while TEM was used to estimate the average size distribution of particles. For the exposure experiment, 48 male rats were divided into four groups (12 rat/group) and gavaged daily with different levels of zero (control), 5, 10, and 20 mg of SiNPs corresponding to zero, 31.25, 62.5, and 125 mg per kg of body weight. Sampling was carried out after 24 and 96 h. Relative to the control group, the exposure to SiNPs induced clear behavioral changes such as inactivity, lethargy, aggressiveness, and screaming. In a dose-dependent manner, the behavior scores recorded the highest values. Pairwise comparisons with the control demonstrated a significant (p < 0.05) decrease in hematological and immunological biomarkers [lysozymes and alternative complement activity (ACH50)] with a concomitant reduction in the antioxidant enzymes [catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)] in all exposed groups to SiNPs. On the contrary, there was a noticeable increase in biochemical parameters (glucose, cortisol, creatinine, urea, low-density lipoproteins (LDL), high-density lipoproteins (HDL), total protein, and albumin) and hepato-renal indicators, including alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), of all SiNP-exposed groups. It was observed that SiNPs induced acute toxicity, either after 24 h or 96 h, post-exposure of rats to SiNPs evidenced by ethological changes, hepato-renal dysfunction, hyperlipemia, and severe suppression in hematological, protein, stress, and immune-antioxidant biomarkers reflecting an impaired physiological status. The obtained outcomes create a foundation for future research to consider the acute toxicity of nanoparticles to preserve human health and sustain the environment.
Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction
Authors: Shimane Yasuhiro , Kuruma Yutetsu
Published: April 2022

Giant vesicles have been widely used for the bottom-up construction of artificial (or synthetic) cells and the physicochemical analysis of lipid membranes. Although methods for the formation of giant vesicles and the encapsulation of molecules within them have been established, a standardized protocol has not been shared among researchers including non-experts. Here we proposed a rapid and facile protocol that allows the formation of giant vesicles within 30 min. The quality of the giant vesicles encapsulating a cell-free protein expression system was comparable to that of the ones formed using a conventional method, in terms of the synthesis of both soluble and membrane proteins. We also performed protein synthesis in artificial cells using a lyophilized cell-free mixture and showed an equivalent level of protein synthesis. Our method could become a standard method for giant vesicle formation suited for artificial cell research.
Whole Body Vibration Attenuates Brain Damage and Neuroinflammation Following Experimental Traumatic Brain Injury
Authors: Chen Tao , Liu Wen-Bo , Ren Xu , Li Yun-Fei , Li Wei , Hang Chun-Hua , Wang Yu-Hai
Published: April 2022

Traumatic brain injury (TBI) is still a major public health problem worldwide, and the research of neuroprotective drugs has encountered great difficulties. Whole body vibration (WBV) is a safe and powerful rehabilitative intervention in various clinical settings, but its effect on neurological diseases is not well documented. In this study, we investigated the effects of WBV pretreatment on brain damage following experimental TBI mimicked by controlled cortical impact (CCI) in mice. C57BL/6 J male mice were expose to WBV at 30 Hz twice per day for 20 days and injured by CCI. WBV had no effect on animal body weight, but significantly reduced the TBI-induced brain edema in the cortex. The results of immunostaining showed that the activation of microglia and astrocytes induced by TBI in brain sections was attenuated by WBV. In consistent, WBV markedly inhibited the expression of pro-inflammatory cytokines, while increased the levels of anti-inflammatory cytokine interleukin 10 (IL-10). In addition, WBV pretreatment alleviated neuronal apoptosis in the cortex and suppressed the cleavage of the apoptotic executive molecule caspase-1. The neurological dysfunction following TBI was determined by open field test and Morris Water Maze (MWM) assay. The results showed that motor activity, learning and memory ability were preserved by WBV compared to TBI-injured mice. In summary, our present data identified WBV as a clinically potent strategy with which to attenuate TBI-related brain damage through regulating neuroinflammation.
Beyond the Channels: Adhesion Functions of Aquaporin 0 and Connexin 50 in Lens Development
Authors: Li Zhen , Quan Yumeng , Gu Sumin , Jiang Jean X.
Published: April 2022

Lens, an avascular tissue involved in light transmission, generates an internal microcirculatory system to promote ion and fluid circulation, thus providing nutrients to internal lens cells and excreting the waste. This unique system makes up for the lack of vasculature and distinctively maintains lens homeostasis and lens fiber cell survival through channels of connexins and other transporters. Aquaporins (AQP) and connexins (Cx) comprise the majority of channels in the lens microcirculation system and are, thus, essential for lens development and transparency. Mutations of AQPs and Cxs result in abnormal channel function and cataract formation. Interestingly, in the last decade or so, increasing evidence has emerged suggesting that in addition to their well-established channel functions, AQP0 and Cx50 play pivotal roles through channel-independent actions in lens development and transparency. Specifically, AQP0 and Cx50 have been shown to have a unique cell adhesion function that mediates lens development and transparency. Precise regulation of cell-matrix and cell-cell adhesion is necessary for cell migration, a critical process during lens development. This review will provide recent advances in basic research of cell adhesion mediated by AQP0 and Cx50.
Automated Bioprocess Feedback Operation in a High-Throughput Facility via the Integration of a Mobile Robotic Lab Assistant
Authors: Kaspersetz Lucas , Waldburger Saskia , Schermeyer M.-Therese , Riedel Sebastian L. , Groß Sebastian , Neubauer Peter , Cruz-Bournazou M.-Nicolas
Published: April 2022

The development of biotechnological processes is challenging due to the diversity of process parameters. For efficient upstream development, parallel cultivation systems have proven to reduce costs and associated timelines successfully while offering excellent process control. However, the degree of automation of such small-scale systems is comparatively low, and necessary sample analysis requires manual steps. Although the subsequent analysis can be performed in a high-throughput manner, the integration of analytical devices remains challenging, especially when cultivation and analysis laboratories are spatially separated. Mobile robots offer a potential solution, but their implementation in research laboratories is not widely adopted. Our approach demonstrates the integration of a small-scale cultivation system into a liquid handling station for an automated cultivation and sample procedure. The samples are transported via a mobile robotic lab assistant and subsequently analyzed by a high-throughput analyzer. The process data are stored in a centralized database. The mobile robotic workflow guarantees a flexible solution for device integration and facilitates automation. Restrictions regarding spatial separation of devices are circumvented, enabling a modular platform throughout different laboratories. The presented cultivation platform is evaluated on the basis of industrially relevant E. coli BW25113 high cell density fed-batch cultivation. The necessary magnesium addition for reaching high cell densities in mineral salt medium is automated via a feedback operation loop between the analysis station located in the adjacent room and the cultivation system. The modular design demonstrates new opportunities for advanced control options and the suitability of the platform for accelerating bioprocess development. This study lays the foundation for a fully integrated facility, where the physical connection of laboratory equipment is achieved through the successful use of a mobile robotic lab assistant, and different cultivation scales can be coupled through the common data infrastructure.
An Improved Cr-EA-IRMS Method for the Effective Determination of the Hydrogen Isotopes in Coal Samples
Authors: Li Hongwei , Feng Lianjun
Published: April 2022

Hydrogen isotope analysis of coal is an important tool in the geochemical analysis of coal. The traditional method of hydrogen isotope analysis of coal requires the oxidation of organic matter bound hydrogen in coal to water by an oxidizing agent and then its reduction to hydrogen by a reducing agent. This method is time-consuming and laborious, and makes it difficult to cope with the rapid detection of large numbers of samples. The recent development of continuous flow IRMS systems (CF-IRMS) has solved the problem of inefficient analysis, but does not guarantee the quantitative conversion of organic bound H to H2, resulting in inaccurate measured hydrogen isotope values. In this study, for the hydrogen isotope analysis of coal, an alternative continuous flow system (Cr-EA-IRMS) was used to achieve high precision hydrogen isotope measurements of coal samples by filling a quartz reaction tube with Cr. The results obtained by this method (−121.3 ± 1.1‰) for the reference material (GBW11104) are consistent with those obtained by the conventional method (−121.4 ± 0.6‰). Using this method, hydrogen isotope measurements for a variety of imported coals revealed significant differences in the hydrogen isotopes of coals from different coal producing regions including Russia, South East Asia, and Australia. Therefore, the use of hydrogen isotope testing analysis of coal could be a potential means of tracing the origin of coal.
Study of the Adsorption Behavior of Surfactants on Carbonate Surface by Experiment and Molecular Dynamics Simulation
Authors: Hou Jinjian , Lin Shuanglong , Du Jinze , Sui Hong
Published: April 2022

Surfactants adsorption onto carbonate reservoirs would cause surfactants concentrations decrease in surfactant flooding, which would decrease surfactant efficiency in practical applications of enhanced oil recovery (EOR) processes. Different surfactants could be classified as cationic surfactants, anionic surfactants, non-ionic surfactants according to the main charge, or be classified as chemical surfactant and bio-surfactant according to the surfactant origin. However, the research on different type surfactants adsorption on carbonate reservoirs surface differences was few. Therefore, five representative surfactants (CTAB, SDS, TX-100, sophorolipid, rhamonilipid) adsorption effect onto carbonate reservoirs surface was studied. Owing to the fact that the salinity and temperature in underground carbonate reservoirs were high during the EOR process, it is vital to study the salinity effect and temperature effect on surfactant adsorption. In this study, different surfactants species, temperature and salinity adsorption onto carbonate reservoirs were studied. The adsorption isotherms were fitted by Langmuir, Freundlich, Temkin and Linear models, and the first three models fitting effect were good. The results showed that cationic surfactants adsorption quantity was higher than anionic surfactants, and the non-ionic surfactants adsorption quantity was the lowest. When the temperature increased, the surfactants adsorption would decrease, because the adsorption process was exothermic process, and increasing temperature would inhibit the adsorption. The higher salinity would increase surfactants adsorption because higher salinity could compress electric double layer. In order to decrease surfactants adsorption, SiO2 nanoparticles and TiO2 nanoparticles were added to surfactants solutions, and then surfactants could adsorb onto nanoparticles surface, then the steric hindrance between surfactant molecules would increase, which could decrease surfactants adsorption. Contact angle results indicated that surfactants adsorption made the carbonate reservoir wettability alteration. In the end, surfactants (with or without SiO2 nanoparticles) adsorption onto carbonate reservoirs mechanism were studied by molecular dynamics simulation. The simulation results indicated that the surfactants molecules could adsorb onto SiO2 nanoparticles surface, and then the surfactants adsorption quantity onto carbonate rocks would decrease, which was in accordance with the experiments results.
Recent Advances in Antimony Sulfide-Based Nanomaterials for High-Performance Sodium-Ion Batteries: A Mini Review
Authors: Wang Guangxin , Guo Mingyi , Zhao Yunchao , Zhao Yibo , Tang Kun , Chen Zhijun , Stock Heinz-Rolf , Liu Yong
Published: April 2022

Recently, sodium-ion batteries (SIBs) have attracted extensive attention as potential alternatives to lithium-ion batteries (LIBs) due to the abundance, even distribution, low cost, and environmentally friendly nature of sodium. However, sodium ions are larger than lithium ions so that the anode materials of LIBs are not suitable for SIBs. Therefore, many negative electrode materials have been investigated. Among them, Sb2S3-based nanomaterials have gradually become a research focus due to their high theoretical specific capacity, good thermal stability, simple preparation, and low price. In this review, the research progress of Sb2S3-based nanomaterials in the SIB field in recent years is summarized, including Sb2S3, Sb2S3/carbon composites, Sb2S3/graphene composites, and Sb2S3/MxSy composites. Furthermore, the challenges and prospects for the development of Sb2S3-based nanomaterials are also put forward. We hope this review will contribute to the design and manufacture of high-performance SIBs and promote its practical application.
The Quantitation of Squalene and Squalane in Bronchoalveolar Lavage Fluid Using Gas Chromatography Mass Spectrometry
Authors: Cowan Elizabeth A. , Tran Hang , Watson Clifford H. , Blount Benjamin C. , Valentín-Blasini Liza
Published: April 2022

Chemicals of unknown inhalational toxicity are present in electronic cigarette and vaping products. E-cigarettes typically contain nicotine and other relatively hydrophilic chemicals while vaping products typically contain cannabinoids and other hydrophobic chemicals. For example, vaping products can include hydrophobic terpenes such as squalane (SQA) and squalene (SQE). However, little is known about the SQA and SQE transmission from liquid to aerosol. SQA and SQE are used in commercial products that are applied dermally and ingested orally, but limited information is available on their inhalational exposure and toxicity. We developed and validated a quantitative method to measure SQE and SQA in bronchoalveolar lavage fluid to assess if these chemicals accumulate in lung epithelial lining fluid after inhalation. Calibration curves spanned a range of 0.50–30.0 µg analyte per mL bronchoalveolar lavage fluid. Recoveries were found to be 97–105% for SQE and 81–106% for SQA. Limits of detection were 0.50 μg/ml for both SQE and SQA. The method was applied to bronchoalveolar lavage fluid samples of patients from the 2019 outbreak of e-cigarette, or vaping, product use-associated lung injury (EVALI) and a comparison group. Neither SQA nor SQE was detected above the method LOD for any samples analyzed; conversely, SQA or SQE were reproducibly measured in spiked quality control BAL fluids (relative standards deviations <15% for both analytes). Further applications of this method may help to evaluate the potential toxicity of SQA and SQE chronically inhaled from EVPs.
Australian Aboriginal Otitis-Prone Children Produce High-Quality Serum IgG to Putative Nontypeable Haemophilus influenzae Vaccine Antigens at Lower Titres Compared to Non-Aboriginal Children
Authors: Clark Sharon L. , Seppanen Elke J. , Kirkham Lea-Ann S. , Novotny Laura A. , Bakaletz Lauren O. , Cripps Allan W. , Corscadden Karli , Coates Harvey , Vijayasekaran Shyan , Richmond Peter C. , Thornton Ruth B.
Published: April 2022

Background

Nontypeable Haemophilus influenzae (NTHi) is the most common bacterial otopathogen associated with otitis media (OM). NTHi persists in biofilms within the middle ears of children with chronic and recurrent OM. Australian Aboriginal children suffer exceptionally high rates of chronic and recurrent OM compared to non-Aboriginal children. NTHi protein vaccines comprised of antigens associated with both adhesion and persistence in a biofilm are under development and could be beneficial for children with chronic and recurrent OM. Understanding the ontogeny of natural antibody development to these antigens provides insight into the value of vaccinating with particular antigens.

Methods

An in-house multiplex fluorescent bead immunoassay was used to measure serum IgG titres and avidity for three putative vaccine antigens: recombinant soluble PilA (rsPilA), ChimV4, and outer membrane protein 26 (OMP26) in sera from Australian Aboriginal otitis-prone children (n=77), non-Aboriginal otitis-prone children (n=70) and non-otitis-prone children (n=36). Serum IgG titres were adjusted for age, and geometric mean concentrations (GMCs) were compared between groups using a univariate analysis model. Antibody avidity was calculated as a relative avidity index and compared between groups using ANOVA.

Results

Australian Aboriginal otitis-prone children had lower serum IgG titres to rsPilA and ChimV4 than non-Aboriginal otitis-prone children (p<0.001), and non-otitis-prone children (p<0.020). No differences were observed between serum IgG titres from non-Aboriginal otitis-prone children and non-otitis-prone children. There were also no differences in the proportion of high avidity IgG specific for these antigens between these groups. Serum IgG titres to OMP26 were similar between all groups (p>0.670) although otitis-prone children had a higher proportion of high avidity antibodies to this antigen.

Conclusions

Australian Aboriginal otitis-prone children had lower serum IgG titres to 2/3 major NTHi vaccine candidate antigens, suggesting these children are unable to develop persistent IgG responses due to repeated NTHi exposure. These reduced IgG titres may relate to earlier and more frequent exposure to diverse NTHi strains in Aboriginal children through carriage or infection. These data suggest that Aboriginal children may benefit from immunisation with vaccines containing these antigens to increase titres of protective antibodies.
Extracellular Vesicles, New Players in Sepsis and Acute Respiratory Distress Syndrome
Authors: Jing Wenqiang , Wang Huijuan , Zhan Liying , Yan Wei
Published: April 2022

Sepsis refers to a complex syndrome associated with physiological, pathological, and biochemical abnormalities resulted from infection. Sepsis is the major cause of acute respiratory distress syndrome (ARDS). Extracellular vesicles (EVs) are serving as new messengers to mediate cell-cell communication in vivo. Non-coding RNAs, proteins and metabolites encapsulated by EVs could result in either pro-inflammatory or anti-inflammatory effects in the recipient cells. Pathogens or host cells derived EVs play an important role in pathogens infection during the occurrence and development of sepsis and ARDS. Additionally, we summarize the potential application for EVs in diagnosis, prevention and treatment for sepsis and ARDS.
AI Ethics as Applied Ethics
Authors: Hallamaa Jaana , Kalliokoski Taina
Published: April 2022

The need to design and develop artificial intelligence (AI) in a sustainable manner has motivated researchers, institutions, and organizations to formulate suggestions for AI ethics. Although these suggestions cover various topics and address diverse audiences, they share the presupposition that AI ethics provides a generalizable basis for designers that is applicable to their work. We propose that one of the reasons the influence of current ethical codes has remained modest, may be the conception of the applied ethics that they represent. We discuss bioethics as a point of reference for weighing the metaethical and methodological approaches adopted in AI ethics, and propose that AI ethics could be made more methodologically solid and substantively more influential if the resources were enriched by adopting tools from fields of study created to improve the quality of human action and safeguard its desired outcomes. The approaches we consider to be useful for this purpose are the systems theory, safety research, impact assessment approach, and theory of change.
Platelet Subtypes in Inflammatory Settings
Authors: Hamad Muataz Ali , Krauel Krystin , Schanze Nancy , Gauchel Nadine , Stachon Peter , Nuehrenberg Thomas , Zurek Mark , Duerschmied Daniel
Published: April 2022

In addition to their essential role in hemostasis and thrombosis, platelets also modulate inflammatory reactions and immune responses. This is achieved by specialized surface receptors as well as secretory products including inflammatory mediators and cytokines. Platelets can support and facilitate the recruitment of leukocytes into inflamed tissue. The various properties of platelet function make it less surprising that circulating platelets are different within one individual. Platelets have different physical properties leading to distinct subtypes of platelets based either on their function (procoagulant, aggregatory, secretory) or their age (reticulated/immature, non-reticulated/mature). To understand the significance of platelet phenotypic variation, qualitatively distinguishable platelet phenotypes should be studied in a variety of physiological and pathological circumstances. The advancement in proteomics instrumentation and tools (such as mass spectrometry-driven approaches) improved the ability to perform studies beyond that of foundational work. Despite the wealth of knowledge around molecular processes in platelets, knowledge gaps in understanding platelet phenotypes in health and disease exist. In this review, we report an overview of the role of platelet subpopulations in inflammation and a selection of tools for investigating the role of platelet subpopulations in inflammation.
Multi-Biomarker Points and Outcomes in Patients Hospitalized for Heart Failure: Insights From the China PEACE Prospective Heart Failure Study
Authors: He Guangda , Zhang Lihua , Huo Xiqian , Wang Qing , Hu Danli , Huang Xinghe , Ge Jinzhuo , Wu Yongjian , Li Jing
Published: April 2022

Objective

To quantitatively characterize the pattern of systemic impairment reflected by conventional biomarkers and assess how it relates to clinical outcomes and quality of life among patients hospitalized for heart failure (HF).

Methods

Patients hospitalized for HF from 52 hospitals in China were enrolled between 2016 and 2018. They were divided into developing and validating cohorts; the developing cohort was used for calculating the weights of biomarkers and constructing the multi-biomarker panel, while the validating one was used for evaluating the relationship between multi-biomarker points and outcomes. In total, five conventional biomarkers reflecting various pathophysiological processes were included in the panel: N-terminal pro-B type natriuretic peptide, high-sensitivity troponin T, hemoglobin, albumin, and creatinine. The weights of the biomarkers were defined based on their relationship with cardiovascular death, and each patient had a multi-biomarker point ranging from 0 to 12. The primary clinical outcome was cardiovascular death, and the other clinical outcomes included rehospitalization for HF, all-cause death, and all-cause rehospitalization in 1-year. The quality of life was measured using Kansas City Cardiovascular Questionnaire. Multi-variable Cox proportional hazard models were used to assess the risks of clinical outcomes, and generalized linear models were used to evaluate the quality of life.

Results

In total, 4,693 patients hospitalized for HF were included in this analysis; the median (interquartile range, IQR) age was 67 (57–75) years old and 1,763 (37.6%) were female. The median multi-biomarker point was 5 (IQR, 2–6). There were 18.0% of patients in the low point group (<2), 29.4% in the mid-low point group (2–4), 27.8% in the mid-high point group (5–6), and 24.7% in the high point group (>6). Compared with those in the low point group, the patients in the high point group had a significantly excess risk of cardiovascular death (adjusted hazard ratio: 5.69, 95% CI, 3.33–9.70). Furthermore, patients with higher points were also more prone to worse quality of life.

Conclusion

Systemic impairment reflected by abnormal conventional biomarker values was common amongst patients hospitalized for HF and had substantially cumulative adverse influence on clinical outcomes and quality of life.
Metabolomics Fingerprint Predicts Risk of Death in Dilated Cardiomyopathy and Heart Failure
Authors: Vignoli Alessia , Fornaro Alessandra , Tenori Leonardo , Castelli Gabriele , Cecconi Elisabetta , Olivotto Iacopo , Marchionni Niccolò , Alterini Brunetto , Luchinat Claudio
Published: April 2022

Background

Heart failure (HF) is a leading cause of morbidity and mortality worldwide. Metabolomics may help refine risk assessment and potentially guide HF management, but dedicated studies are few. This study aims at stratifying the long-term risk of death in a cohort of patients affected by HF due to dilated cardiomyopathy (DCM) using serum metabolomics via nuclear magnetic resonance (NMR) spectroscopy.

Methods

A cohort of 106 patients with HF due to DCM, diagnosed and monitored between 1982 and 2011, were consecutively enrolled between 2010 and 2012, and a serum sample was collected from each participant. Each patient underwent half-yearly clinical assessments, and survival status at the last follow-up visit in 2019 was recorded. The NMR serum metabolomic profiles were retrospectively analyzed to evaluate the patient's risk of death. Overall, 26 patients died during the 8-years of the study.

Results

The metabolomic fingerprint at enrollment was powerful in discriminating patients who died (HR 5.71, p = 0.00002), even when adjusted for potential covariates. The outcome prediction of metabolomics surpassed that of N-terminal pro b-type natriuretic peptide (NT-proBNP) (HR 2.97, p = 0.005). Metabolomic fingerprinting was able to sub-stratify the risk of death in patients with both preserved/mid-range and reduced ejection fraction [hazard ratio (HR) 3.46, p = 0.03; HR 6.01, p = 0.004, respectively]. Metabolomics and left ventricular ejection fraction (LVEF), combined in a score, proved to be synergistic in predicting survival (HR 8.09, p = 0.0000004).

Conclusions

Metabolomic analysis via NMR enables fast and reproducible characterization of the serum metabolic fingerprint associated with poor prognosis in the HF setting. Our data suggest the importance of integrating several risk parameters to early identify HF patients at high-risk of poor outcomes.
Surgical Turned-Downed CHIP Cases—Can PCI Save the Day?
Authors: Achim Alexandru , Marc Madalin , Ruzsa Zoltan
Published: April 2022

Current guidelines, rarely if at all, address decision-making for revascularization when bypass surgery is not a possibility for high-risk cases. Patients who are surgically turned down are routinely excluded from clinical trials, even though they remain symptomatic. Furthermore, the reasons for surgical ineligibility are often times not captured in standardized risk models. There is no data regarding health status outcomes following PCI procedures in these patients and the ultimate question remains whether the benefits of PCI outweigh its risks in this controversial subpopulation. When CHIP (Complex High risk Indicated Percutaneous coronary interventions) is selected for these very complex individuals, there is no unanimity regarding the goals for interventional revascularization (for instance, the ambition to achieve completeness of revascularization vs. more targeted or selective PCI). The recognition that, worldwide, these patients are becoming increasingly prevalent and increasingly commonplace in the cardiac catheterization labs, along with the momentum for more complex interventional procedures and expanding skillsets, gives us a timely opportunity to better examine the outcomes for these patients and inform clinical decision-making.
The Roles of Cardiac Fibroblasts and Endothelial Cells in Myocarditis
Authors: Xuan Yunling , Chen Chen , Wen Zheng , Wang Dao Wen
Published: April 2022

In myocarditis caused by various etiologies, activated immune cells and the immune regulatory factors released by them play important roles. But in this complex microenvironment, non-immune cells and non-cardiomyocytes in the heart, such as cardiomyocytes (CMs), cardiac fibroblasts (CFs) and endothelial cells (ECs), play the role of “sentinel”, amplify inflammation, and interact with the cardiomyocytes. The complex interactions between them are rarely paid attention to. This review will re-examine the functions of CFs and ECs in the pathological conditions of myocarditis and their direct and indirect interactions with CMs, in order to have a more comprehensive understanding of the pathogenesis of myocarditis and better guide the drug development and clinical treatment of myocarditis.
Public Service and Public Happiness: Inferences From Big Weibo Datasets for 31 Chinese Provincial Governments
Authors: Ding Yi Zhou
Published: April 2022

The change of public service has usually been considered to affect public happiness. However, since the publication of the Easterlin Paradox, the causal relationship between public service and public happiness has been furiously questioned by public affairs researchers. It has been documented through resolving the four causal factors of public happiness within public administration, new public administration, new public management, and governance that public-service-driven public happiness may be attributed to four happiness dimensions: Objective Reality, Subjective Reality, Inter-Subjective Reality, and Virtual Reality. This article reports the results of significance tests of the relationship between public service and public happiness from analyses of large datasets collected from Weibo systems in 31 Chinese provincial governments from 2010 to 2020. The analyses show that the public service change during this period has not yet led to satisfactory improvement in all four happiness dimensions. Finally, we propose strategies for governments to modify public services to enhance public happiness.
Gaming My Way to Recovery: A Systematic Scoping Review of Digital Game Interventions for Young People's Mental Health Treatment and Promotion
Authors: Ferrari Manuela , Sabetti Judith , McIlwaine Sarah V. , Fazeli Sahar , Sadati S. M. Hani , Shah Jai L. , Archie Suzanne , Boydell Katherine M. , Lal Shalini , Henderson Joanna , Alvarez-Jimenez Mario , Andersson Neil , Nielsen Rune Kristian Lundedal , Reynolds Jennifer A. , Iyer Srividya N.
Published: April 2022

Nearly all young people use the internet daily. Many youth with mental health concerns, especially since the Covid-19 pandemic, are using this route to seek help, whether through digital mental health treatment, illness prevention tools, or supports for mental wellbeing. Videogames also have wide appeal among young people, including those who receive mental health services. This review identifies the literature on videogame interventions for young people, ages 12-29, and maps the data on game use by those with mental health and substance use problems, focusing on evidence for the capacity of games to support treatment in youth mental health services; how stakeholders are involved in developing or evaluating games; and any potential harms and ethical remedies identified. A systematic scoping review methodology was used to identify and assess relevant studies. A search of multiple databases identified a total of 8,733 articles. They were screened, and 49 studies testing 32 digital games retained. An adapted stepped care model, including four levels, or steps, based on illness manifestation and severity, was used as a conceptual framework for organizing target populations, mental health conditions and corresponding digital games, and study results. The 49 selected studies included: 10 studies (20.4%) on mental health promotion/prevention or education for undiagnosed youth (Step 0: 7 games); 6 studies (12.2%) on at-risk groups or suspected mental problems (Step 1: 5 games); 24 studies (49.0%) on mild to moderate mental conditions (Steps 2-3: 16 games); and 9 studies (18.4%) focused on severe and complex mental conditions (Step 4: 7 games). Two interventions were played by youth at more than one level of illness severity: the SPARX game (Steps 1, 2-3, 4) and Dojo (Steps 2-3 and 4), bringing the total game count to 35 with these repetitions. Findings support the potential integration of digital games in youth services based on study outcomes, user satisfaction, relatively high program retention rates and the potential usefulness of most games for mental health treatment or promotion/prevention. Most studies included stakeholder feedback, and involvement ratings were very high for seven games. Potential harms were not addressed in this body of research. This review provides an important initial repository and evaluation of videogames for use in clinical settings concerned with youth mental health.
Application of Time-Variable Gravity to Groundwater Storage Fluctuations in Saudi Arabia
Authors: Mohamed Ahmed , Abdelrahman Kamal , Abdelrady Ahmed
Published: April 2022

In the Middle East, water shortage is becoming more and more serious due to the development of agriculture and industry and the increase in population. Saudi Arabia is one of the most water-consuming countries in the Middle East, and urgent measures are needed. Therefore, we integrated data from Gravity Recovery and Climate Experiment (GRACE), and other relevant data to estimate changes in groundwater storage in Saudi Arabia. The findings are as follows: 1) Average annual precipitation (AAP) was calculated to be 76.4, 90, and 72 mm for the entire period, Period I (April 2002 to March 2006) and Period II (April 2006 to July 2016), respectively. 2) The average TWS variation was estimated to be −7.94 ± 0.22, −1.39 ± 1.35, and −8.38 ± 0.34 mm/yr for the entire period, Period I and Period II, respectively. 3) The average groundwater storage was estimated to be +1.56 ± 1.35 mm/yr during Period I. 4) The higher average groundwater depletion rate was calculated to be −6.05 ± 0.34 mm/yr during Period II. 5) Both soil texture and surface streams in the study area promote lateral flow and carry surface water to the Arabian Gulf and the Red Sea. 6) During Period II, average annual recharge rates were estimated to be +9.48 ± 2.37 and +4.20 ± 0.15 km3 for Saudi Arabia and the Saq aquifer, respectively. 7) This integrated approach is an informative and cost-effective technique to assess the variability of groundwater resources in large areas more efficiently.
A Systematic Review of the Provision of Sexuality Education to Student Teachers in Initial Teacher Education
Authors: Costello Aisling , Maunsell Catherine , Cullen Claire , Bourke Ashling
Published: April 2022

Teachers, and their professional learning and development, have been identified as playing an integral role in enabling children and young people’s right to comprehensive sexuality education (CSE). The provision of sexuality education (SE) during initial teacher education (ITE) is upheld internationally, as playing a crucial role in relation to the implementation and quality of school-based SE. This systematic review reports on empirical studies published in English from 1990 to 2019. In accordance with the PRISMA guidelines, five databases were searched: ERIC, Education Research Complete, PsycINFO, Web of Science and MEDLINE. From a possible 1,153 titles and abstracts identified, 15 papers were selected for review. Findings are reported in relation to theWHO Regional Office for Europe and BZgA (2017)Training Matters: Framework of core competencies for sexuality educators. Results revealed that research on SE during ITE is limited and minimal research has focused on student teachers’ attitudes on SE. Findings indicate that SE provision received is varied and not reflective of comprehensive SE. Recommendations highlight the need for robust research to inform quality teacher professional development practices to support teachers to develop the knowledge, attitudes and skills necessary to teach comprehensive SE.
Willingness to Receive COVID-19 Vaccine and Associated Factors Among College Students in Gondar City, Northwest Ethiopia
Authors: Aklil Mastewal Belayneh , Temesgan Wubedle Zelalem , Gessesse Dereje Nibret , Kassa Bekalu Getnet , Tiguh Agumas Eskezia , Kebede Azmeraw Ambachew , Ayalew Minychil Demelash , Yismaw Ayenew Engida
Published: April 2022

Background

The increasing incidence of coronavirus disease 2019 (COVID-19) continues to cause morbidities, mortalities, and economic crises worldwide. College students are among the high-risk groups to acquire the disease for many reasons. Thus, the COVID-19 vaccine is the most effective and safe method to control this deadly disease. Although World Health Organization advocates vaccination is the best approach to tackle COVID-19, refusal of vaccination is a global problem. Evidence of reaching out to college students is scarce in Ethiopia, particularly in the study area. Therefore, this study aimed to assess willingness to receive the COVID-19 vaccine and associated factors among college students in northwest Ethiopia.

Methods

An institution-based cross-sectional study was conducted among 626 participants from January 12–26, 2021. Data were collected using a pretested, self-administered questionnaire, and a multistage sampling technique was used. Data were entered into Epi Info version 7.1.5.2 and exported into SPSS version 20 for analysis. Both bivariable and multivariable logistic regression analyses were used to identify associated factors. The level of significance was determined based on a p-value < 0.05.

Results

In this study, the student’s willingness to receive COVID-19 vaccine was 34.2% [95% confidence interval (CI): 30.9–38.1]. Being third-year student [adjusted odds ratio (AOR) = 1.88; 95% CI: 1.27–2.77], being male (AOR = 1.45; 95% CI: 1.02–2.09), being married (AOR = 2.07; 95% CI: 1.28–3.33), ever watching TV (AOR = 0.39; 95% CI: 0.24–0.65), and having a positive attitude toward COVID-19 prevention (AOR = 2.33; 95% CI: 1.61–3.39) were the factors significantly associated with the willingness to receive the COVID-19 vaccine.

Conclusion

Willingness to receive the COVID-19 vaccine among college students was low. Sensitization of students about COVID-19 vaccine safety and effectiveness before directly giving the vaccine is recommended. Moreover, concerned bodies should provide improved health education to students to bring a favorable attitude toward the COVID-19 vaccine.
Teachers’ Perceptions of Their Role and Classroom Management Practices in a Technology Rich Primary School Classroom
Authors: Johler Minttu , Krumsvik Rune Johan , Bugge Hans Erik , Helgevold Nina
Published: April 2022

This case study investigates primary school teachers’ perceptions of their role and practices regarding classroom management in technology-rich classrooms. The data was collected through individual and focus group interviews, observation and a survey at a school where implementation of digital technologies has been a high priority over several years. The study identifies complexity and contemporary elements in teachers’ perceived role and practices, as the rapid evolution of ICT requires teachers to constantly keep up-to-date, gain new competencies and evaluate their practices to be able to facilitate learning in physical classrooms that have expanded to the digital space. In this process, the role of leadership, collegial collaboration, good teacher-pupil relationships and teachers’ ability to adapt and take up a role of a learner have been found pivotal.
Electronic Tuning in URu2Si2 Through Ru to Pt Chemical Substitution
Authors: Chappell Greta L. , Nelson William L. , Graf D. E. , Baumbach Ryan E.
Published: April 2022

Studies that control the unit cell volume and electronic composition have been useful in revealing what factors lead to hidden order and superconductivity in the strongly correlated electron system URu2Si2. For example, isoelectronic tuning that increases the hybridization between the f and conduction electron states (i.e., applied pressure and Ru → Fe/Os chemical substitution) 1) converts hidden order into antiferromagnetism and 2) destroys the superconductivity. The impact of nonisoelectronic chemical substitution has been less clear, but several unifying trends have recently emerged for chemical substitution vectors that qualitatively add electrons (e.g., Ru → Rh/Ir and Si → P). This includes 1) the rapid destruction of hidden order and superconductivity, 2) composition regions where the underlying Kondo lattice is preserved but does not harbor an ordered state, and 3) the emergence of complex magnetism at large substitutions. In order to assess the limits of this perspective, we have investigated the series U(Ru1−xPtx)2Si2 for x ≲ 0.19, where the Ru and Pt d-shells differ substantially from each other. Magnetic susceptibility, electrical resistivity, and heat capacity measurements unexpectedly reveal a phase diagram with notable similarities to those of other electron doping series. This result reinforces the viewpoint that there is a quasi-universal affect that results from electron doping in this material, and we anticipate that an understanding of these trends will be useful to isolate what factors are foundational for hidden order and superconductivity.
Loss of Adipocyte STAT5 Confers Increased Depot-Specific Adiposity in Male and Female Mice That Is Not Associated With Altered Adipose Tissue Lipolysis
Authors: Richard Allison J. , Hang Hardy , Allerton Timothy D. , Zhao Peng , Mendoza Tamra , Ghosh Sujoy , Elks Carrie M. , Stephens Jacqueline M.
Published: April 2022

STATs (Signal Transducers and Activators of Transcription) 5A and 5B are induced during adipocyte differentiation and are primarily activated by growth hormone (GH) and prolactin in fat cells. Previous studies in mice lacking adipocyte GH receptor or STAT5 support their roles in lipolysis-mediated reduction of adipose tissue mass. Male and female mice harboring adipocyte-specific deletion of both STAT5 genes (STAT5AKO) exhibit increased subcutaneous or inguinal adipose tissue mass, but no changes in visceral or gonadal fat mass. Both depots display substantial increases in adipocyte size with no changes in lipolysis in adipose tissue explants. RNA sequencing analysis of subcutaneous adipose tissue and indirect calorimetry experiments reveal sex-dependent differences in adipose gene expression and whole-body energy expenditure, respectively, resulting from the loss of adipocyte STAT5.