bluuebunny/crossref_metadata_2025 huggingface dataset:
Dedifferentiation of Immortalized Human Podocytes in Response to Transforming Growth Factor-β
Authors: Herman-Edelstein Michal , Thomas Merlin C. , Thallas-Bonke Vicki , Saleem Moin , Cooper Mark E. , Kantharidis Phillip
Published: April 2011

OBJECTIVE

Diabetic nephropathy is associated with dedifferentiation of podocytes, losing the specialized features required for efficient glomerular function and acquiring a number of profibrotic, proinflammatory, and proliferative features. These result from tight junction and cytoskeletal rearrangement, augmented proliferation, and apoptosis.

RESEARCH DESIGN AND METHODS

Experiments were performed in conditionally immortalized human podocytes developed by transfection with the temperature-sensitive SV40-T gene. Cells were then cultured in the presence of transforming growth factor (TGF)-β1 or angiotensin II in the presence or absence of a selective inhibitor of the TGF-β type I receptor kinase, SB-431542. Gene and protein expression were then examined by real-time RT-PCR and immunofluorescence, and correlated with changes observed in vivo in experimental diabetes.

RESULTS

Treatment of cells with TGF-β1 resulted in dynamic changes in their morphology, starting with retraction and shortening of foot processes and finishing with the formation of broad and complex tight junctions between adjacent podocytes. This dedifferentiation was also associated with dose- and time-dependent reduction in the expression of glomerular epithelial markers (nephrin, p-cadherin, zonnula occludens-1) and increased expression of mesenchymal markers (α−smooth muscle actin, vimentin, nestin), matrix components (fibronectin, collagen I, and collagen IV α3), cellular proliferation, and apoptosis. The induction of diabetes in mice was also associated with similar changes in morphology, protein expression, and proliferation in glomerular podocytes.

CONCLUSIONS

In response to TGF-β and other TGF-dependent stimuli, mature podocytes undergo dedifferentiation that leads to effacement of foot processes, morphologic flattening, and increased formation of intercellular tight junctions. This simplification of their phenotype to a more embryonic form is also associated with reentry of mature podocytes into the cell cycle, which results in enhanced proliferation and apoptosis. These “pathoadaptive” changes are seen early in the diabetic glomerulus and ultimately contribute to albuminuria, glomerulosclerosis, and podocytopenia.
HLA-A2–Matched Peripheral Blood Mononuclear Cells From Type 1 Diabetic Patients, but Not Nondiabetic Donors, Transfer Insulitis to NOD-scid/γcnull/HLA-A2 Transgenic Mice Concurrent With the Expansion of Islet-Specific CD8+ T cells
Authors: Whitfield-Larry Fatima , Young Ellen F. , Talmage Garrick , Fudge Elizabeth , Azam Anita , Patel Shipra , Largay Joseph , Byrd Warren , Buse John , Calikoglu Ali S. , Shultz Leonard D. , Frelinger Jeffrey A.
Published: April 2011

OBJECTIVE

Type 1 diabetes is an autoimmune disease characterized by the destruction of insulin-producing β-cells. NOD mice provide a useful tool for understanding disease pathogenesis and progression. Although much has been learned from studies with NOD mice, increased understanding of human type 1 diabetes can be gained by evaluating the pathogenic potential of human diabetogenic effector cells in vivo. Therefore, our objective in this study was to develop a small-animal model using human effector cells to study type 1 diabetes.

RESEARCH DESIGN AND METHODS

We adoptively transferred HLA-A2–matched peripheral blood mononuclear cells (PBMCs) from type 1 diabetic patients and nondiabetic control subjects into transgenic NOD-scid/γcnull/HLA-A*0201 (NOD-scid/γcnull/A2) mice. At various times after adoptive transfer, we determined the ability of these mice to support the survival and proliferation of the human lymphoid cells. Human lymphocytes were isolated and assessed from the blood, spleen, pancreatic lymph node and islets of NOD-scid/γcnull/A2 mice after transfer.

RESULTS

Human T and B cells proliferate and survive for at least 6 weeks and were recovered from the blood, spleen, draining pancreatic lymph node, and most importantly, islets of NOD-scid/γcnull/A2 mice. Lymphocytes from type 1 diabetic patients preferentially infiltrate the islets of NOD-scid/γcnull/A2 mice. In contrast, PBMCs from nondiabetic HLA-A2–matched donors showed significantly less islet infiltration. Moreover, in mice that received PBMCs from type 1 diabetic patients, we identified epitope-specific CD8+ T cells among the islet infiltrates.

CONCLUSIONS

We show that insulitis is transferred to NOD-scid/γcnull/A2 mice that received HLA-A2–matched PBMCs from type 1 diabetic patients. In addition, many of the infiltrating CD8+ T cells are epitope-specific and produce interferon-γ after in vitro peptide stimulation. This indicates that NOD-scid/γcnull/A2 mice transferred with HLA-A2–matched PBMCs from type 1 diabetic patients may serve as a useful tool for studying epitope-specific T-cell–mediated responses in patients with type 1 diabetes.
Medical Expenditures Associated With Diabetes Among Privately Insured U.S. Youth in 2007
Authors: Shrestha Sundar S. , Zhang Ping , Albright Ann , Imperatore Giuseppina
Published: April 2011

OBJECTIVE

To estimate, among privately insured youth in the U.S., medical expenditures associated with diabetes and the difference in medical expenditures between individuals with insulin-treated diabetes mellitus (ITDM) and with non-ITDM (NITDM).

RESEARCH DESIGN AND METHODS

Using the 2007 MarketScan commercial claims and encounter database, we analyzed data for 49,356 youth (aged ≤19 years) who were continuously enrolled in fee-for-service health plans. Youth with diabetes (cases) were identified from inpatient, outpatient, and pharmaceutical drug claims. Each case was matched with five controls (without diabetes) by age (±2 years), sex, census region, and urban versus rural residence. We used regression models to estimate medical expenditures in total and by component (inpatient, outpatient, and medication).

RESULTS

The predicted mean annual total per-person medical expenditures were $9,061 for youth with diabetes and $1,468 for those without, an excess of $7,593 for those with diabetes; of which, 43% was for prescription drugs. The predicted mean annual total expenditures were $9,333 for ITDM youth and $5,683 for NITDM youth, respectively, an excess of $3,650 for those with ITDM diabetes, of which 59% was for prescription drugs.

CONCLUSIONS

The excess medical expenditures associated with diabetes, ITDM in particular, among youth are substantial. Our estimates of excess expenditures can be used to assess the economic burden of diabetes overall and by diabetes treatment mode. Our estimated excess expenditure for NITDM may be used for evaluating the economic efficiency of interventions aimed at preventing type 2 diabetes in U.S. youth.
Postprandial Hyperglycemia and Glycemic Variability
Authors: Standl Eberhard , Schnell Oliver , Ceriello Antonio
Published: April 2011

The aim of this article is to evaluate the pros and cons of a specific impact of postprandial hyperglycemia and glycemic variability on the—mainly cardiovascular (CV)—complications of diabetes, above and beyond the average blood glucose (BG) as measured by HbA1c or fasting plasma glucose (FPG). The strongest arguments in favor of this hypothesis come from impressive pathophysiological studies, also in the human situation. Measures of oxidative stress and endothelial dysfunction seem to be especially closely related to glucose peaks and even more so to fluctuating high and low glucose concentrations and can be restored to normal by preventing those glucose peaks or wide glucose excursions. The epidemiological evidence, which is more or less confined to postprandial hyperglycemia and postglucose load glycemia, is also rather compelling in favor of the hypothesis, although certainly not fully conclusive as there are also a number of conflicting results. The strongest cons are seen in the missing evidence as derived from randomized prospective intervention studies targeting postprandial hyperglycemia longer term, i.e., over several years, and seeking to reduce hard CV end points. In fact, several such intervention studies in men have recently failed to produce the intended beneficial outcome results. As this evidence by intervention is, however, key for the ultimate approval of a treatment concept in patients with diabetes, the current net balance of attained evidence is not in favor of the hypothesis here under debate, i.e., that we should care about postprandial hyperglycemia and glycemic variability. The absence of a uniformly accepted standard of how to estimate these parameters adds a further challenge to this whole debate.
Type 2 Diabetes Can Be Prevented With Early Pharmacological Intervention
Authors: DeFronzo Ralph A. , Abdul-Ghani Muhammad
Published: April 2011

In the U.S., ∼21 × 106 individuals have type 2 diabetes, and twice as many have impaired glucose tolerance (IGT). Approximately 40–50% of individuals with IGT will progress to type 2 diabetes over their lifetime. Therefore, treatment of high-risk individuals with IGT to prevent type 2 diabetes has important medical, economic, social, and human implications. Weight loss, although effective in reducing the conversion of IGT to type 2 diabetes, is difficult to achieve and maintain. Moreover, 40–50% of IGT subjects progress to type 2 diabetes despite successful weight reduction. In contrast, pharmacological treatment of IGT with oral antidiabetic agents that improve insulin sensitivity and preserve β-cell function—the characteristic pathophysiological abnormalities present in IGT and type 2 diabetes—uniformly have been shown to prevent progression of IGT to type 2 diabetes. The most consistent results have been observed with the thiazolidinediones (Troglitazone in the Prevention of Diabetes [TRIPOD], Pioglitazone in the Prevention of Diabetes [PIPOD], Diabetes Reduction Assessment with Ramipril and Rosiglitazone Medication [DREAM], and Actos Now for the Prevention of Diabetes [ACT NOW]), with a 50–70% reduction in IGT conversion to diabetes. Metformin in the U.S. Diabetes Prevention Program (DPP) reduced the development of type 2 diabetes by 31% and has been recommended by the American Diabetes Association (ADA) for treating high-risk individuals with IGT. The glucagon-like peptide-1 analogs, which augment insulin secretion, preserve β-cell function, and promote weight loss, also would be expected to be efficacious in preventing the progression of IGT to type 2 diabetes. Because individuals in the upper tertile of IGT are maximally/near-maximally insulin resistant, have lost 70–80% of their β-cell function, and have an ∼10% incidence of diabetic retinopathy, pharmacological intervention, in combination with diet plus exercise, should be instituted.
Benefits of Tight Blood Pressure Control in Diabetic Patients With Hypertension
Authors: Parati Gianfranco , Bilo Grzegorz , Ochoa Juan E.
Published: April 2011

In 2008, when the UK Prospective Diabetes Study (UKPDS) group presented their 30-year findings concerning the possible sustained effects of improved glycemic control after 10 years of extended follow-up in type 2 diabetic patients, a so-called “legacy effect” was reported to address the long-term emergent and/or sustained benefits of early improved glycemic control. Opposite results were obtained by the Hypertension in Diabetes Study (HDS) carried out in the frame of UKPDS, with no evidence of any legacy effect on cardiovascular (CV) outcomes for an initial 4-year period of tight blood pressure (BP) control. Thus, it was concluded that BP control has to be continued over time, since, although it had a short time-to-effect relationship in preventing stroke, BP control was associated with a short persistence of its clinical benefits once the intervention was discontinued. These findings are unique because, whereas most interventional trials in hypertension that included diabetic patients have shown a reduction in CV outcomes shortly after starting treatment, only the UKPDS-HDS specifically explored the possible persistence of clinical benefits after discontinuing intensive BP-lowering intervention. This article aims to provide a critical interpretation of the UKPDS findings of lack of BP legacy, in the context of the currently available evidence on the benefits of antihypertensive treatment. The importance of effective BP control in type 2 diabetic patients to prevent CV outcomes and other diabetes-related complications is underlined, with emphasis on early, tight, and continuous BP control to optimize patients’ protection.
The five-variable Volterra system
Authors: Zieliński Janusz
Published: April 2011

We give a description of all polynomial constants of the five-variable Volterra derivation, hence of all polynomial first integrals of its corresponding Volterra system of differential equations. The Volterra system plays a significant role in plasma physics and population biology.
Matrix metalloproteinases at key junctions in the pathomechanism of stroke
Authors: Rottenberger Zsolt , Kolev Krasimir
Published: April 2011

Matrix metalloproteinases play a crucial role in the remodelling of the extracellular matrix through direct degradation of its structural proteins and control of extracellular signalling. The most common cause of ischemic brain damage is an atherothrombotic lesion in the supplying arteries. The progress of the atherosclerotic plaque development and the related thrombotic complications are mediated in part by matrix metalloproteinases. In addition to their role in the underlying disease, various members of this protease family are upregulated in the acute phase of ischemic brain damage as well as in the post-ischemic brain recovery following stroke. This review summarizes the current understanding of the matrix metalloproteinase-related molecular events at three stages of the ischemic cerebrovascular disease (in the atherosclerotic plaque, in the neurovascular unit of the brain and in the regenerating brain tissue).
Receiving an Alcohol Enquiry from a Physician in Routine Health Care in Sweden: A Population-Based Study of Gender Differences and Predictors
Authors: Engdahl Barbro , Nilsen Per
Published: April 2011

Research has shown that the provision of brief interventions in the health care system is effective for reducing hazardous drinking. Using a telephone-administered questionnaire, this study provides a population-based investigation on the extent to which physicians address patients’ alcohol habits in the Swedish health care system, whether there are gender differences in the extent to which patients receive questions about alcohol, and predictors for receiving such questions. Data were obtained from monthly telephone surveys with around 72,000 people in 2006–2009. Having received an alcohol enquiry was defined as having been asked about one’s drinking habits by a physician in any health care visit in the last 12 months. Fourteen percent of the total population had received an alcohol enquiry, but there were considerable gender differences: for hazardous drinkers, 13% of the women and 17% of the men had received an alcohol enquiry; among those with sensible alcohol consumption, 10% of women and 15% of men had received an alcohol enquiry. Patients were more likely to have received an alcohol enquiry if they had self-reported alcohol-related problems, were hazardous drinkers and/or daily smokers. Some of the alcohol enquiry predictors differed by gender; social class was an important predictor for women but not for men.
Effects of Oral Glucosamine Hydrochloride Administration on Plasma Free Amino Acid Concentrations in Dogs
Authors: Azuma Kazuo , Osaki Tomohiro , Tsuka Takeshi , Imagawa Tomohiro , Okamoto Yoshiharu , Takamori Yoshimori , Minami Saburo
Published: April 2011

We examined the effects of oral glucosamine hydrochloride (GlcN), N-acetyl-D-glucosamine (GlcNAc) and D-glucose (Glc) administration on plasma total free amino acid (PFAA) concentrations in dogs. The PFAA concentrations increased in the control group and the GlcNAc group at one hour after feeding, and each amino acid concentration increased. On the other hand, in the GlcN group and the Glc group PFAA concentrations decreased at one hour after feeding. A significant decrease in amino acid concentration was observed for glutamate, glycine and alanine. Our results suggest the existence of differences in PFAA dynamics after oral administration of GlcN and GlcNAc in dogs.
Effect of Lectins from Diocleinae Subtribe against Oral Streptococci
Authors: Cavalcante Theodora Thays Arruda , Anderson Matias da Rocha Bruno , Alves Carneiro Victor , Vassiliepe Sousa Arruda Francisco , Fernandes do Nascimento Antônia Sâmia , Cardoso Sá Nairley , Do Nascimento Kyria Santiago , Sousa Cavada Benildo , Holanda Teixeira Edson
Published: April 2011

Surface colonization is an essential step in biofilm development. The ability of oral pathogens to adhere to tooth surfaces is directly linked with the presence of specific molecules at the bacterial surface that can interact with enamel acquired pellicle ligands. In light of this, the aim of this study was to verify inhibitory and antibiofilm action of lectins from the Diocleinaesubtribe against Streptococcus mutans and Streptococcus oralis. The inhibitory action against planctonic cells was assessed using lectins from Canavaliaensi formis (ConA), Canavalia brasiliensis (ConBr), Canavalia maritima (ConM), Canavalia gladiata (CGL) and Canavalia boliviana (ConBol). ConBol, ConBr and ConM showed inhibitory activity on S. mutans growth. All lectins, except ConA, stimulated significantly the growth of S. oralis. To evaluate the effect on biofilm formation, clarified saliva was added to 96-well, flat-bottomed polystyrene plates, followed by the addition of solutions containing 100 or 200 µg/mL of the selected lectins. ConBol, ConM and ConA inhibited the S. mutans biofilms. No effects were found on S. oralis biofilms. Structure/function analysis were carried out using bioinformatics tools. The aperture and deepness of the CRD (Carbohydrate Recognition Domain) permit us to distinguish the two groups of Canavalia lectins in accordance to their actions against S. mutans and S. oralis. The results found provide a basis for encouraging the use of plant lectins as biotechnological tools in ecological control and prevention of caries disease.
Multi-Temporal Land-Cover Classification of Agricultural Areas in Two European Regions with High Resolution Spotlight TerraSAR-X Data
Authors: Bargiel Damian , Herrmann Sylvia
Published: April 2011

Functioning ecosystems offer multiple services for human well-being (e.g., food, freshwater, fiber). Agriculture provides several of these services but also can cause negative impacts. Thus, it is essential to derive up-to-date information about agricultural land use and its change. This paper describes the multi-temporal classification of agricultural land use based on high resolution spotlight TerraSAR-X images. A stack of l4 dual-polarized radar images taken during the vegetation season have been used for two different study areas (North of Germany and Southeast Poland). They represent extremely diverse regions with regard to their population density, agricultural management, as well as geological and geomorphological conditions. Thereby, the transferability of the classification method for different regions is tested. The Maximum Likelihood classification is based on a high amount of ground truth samples. Classification accuracies differ in both regions. Overall accuracy for all classes for the German area is 61.78% and 39.25% for the Polish region. Accuracies improved notably for both regions (about 90%) when single vegetation classes were merged into groups of classes. Such regular land use classifications, applicable for different European agricultural sites, can serve as basis for monitoring systems for agricultural land use and its related ecosystems.
Remote Sensing of Mangrove Ecosystems: A Review
Authors: Kuenzer Claudia , Bluemel Andrea , Gebhardt Steffen , Quoc Tuan Vo , Dech Stefan
Published: April 2011

Mangrove ecosystems dominate the coastal wetlands of tropical and subtropical regions throughout the world. They provide various ecological and economical ecosystem services contributing to coastal erosion protection, water filtration, provision of areas for fish and shrimp breeding, provision of building material and medicinal ingredients, and the attraction of tourists, amongst many other factors. At the same time, mangroves belong to the most threatened and vulnerable ecosystems worldwide and experienced a dramatic decline during the last half century. International programs, such as the Ramsar Convention on Wetlands or the Kyoto Protocol, underscore the importance of immediate protection measures and conservation activities to prevent the further loss of mangroves. In this context, remote sensing is the tool of choice to provide spatio-temporal information on mangrove ecosystem distribution, species differentiation, health status, and ongoing changes of mangrove populations. Such studies can be based on various sensors, ranging from aerial photography to high- and medium-resolution optical imagery and from hyperspectral data to active microwave (SAR) data. Remote-sensing techniques have demonstrated a high potential to detect, identify, map, and monitor mangrove conditions and changes during the last two decades, which is reflected by the large number of scientific papers published on this topic. To our knowledge, a recent review paper on the remote sensing of mangroves does not exist, although mangrove ecosystems have become the focus of attention in the context of current climate change and discussions of the services provided by these ecosystems. Also, climate change-related remote-sensing studies in coastal zones have increased drastically in recent years. The aim of this review paper is to provide a comprehensive overview and sound summary of all of the work undertaken, addressing the variety of remotely sensed data applied for mangrove ecosystem mapping, as well as the numerous methods and techniques used for data analyses, and to further discuss their potential and limitations.
Next Generation AT-Cut Quartz Crystal Sensing Devices
Authors: Matko Vojko
Published: April 2011

Generally, AT-cut quartz crystals have a limited scope of use when it comes to high-precision measurement of very small impedance changes due to their nonlinear frequency-temperature characteristics in the range between 0 °C and 50 °C. The new method improving quartz oscillator frequency-temperature characteristic compensation is switching between two impedance loads. By modifying the oscillator circuit with two logic switches and two impedance loads, the oscillator can switch oscillation between two resonance frequencies. The difference in resonance frequencies compensates the frequency-temperature characteristics influence as well as the influence of offset and quartz crystal ageing. The experimental results show that the new approach using the switching method highly improves second-to-second frequency stability from ±0.125 Hz to ±0.00001 Hz and minute-to-minute frequency stability from 0.1 Hz to 0.0001 Hz, which makes the high-precision measurement of aF and fH changes possible.
Dynamic Experiment Design Regularization Approach to Adaptive Imaging with Array Radar/SAR Sensor Systems
Authors: Shkvarko Yuriy , Tuxpan José , Santos Stewart
Published: April 2011

We consider a problem of high-resolution array radar/SAR imaging formalized in terms of a nonlinear ill-posed inverse problem of nonparametric estimation of the power spatial spectrum pattern (SSP) of the random wavefield scattered from a remotely sensed scene observed through a kernel signal formation operator and contaminated with random Gaussian noise. First, the Sobolev-type solution space is constructed to specify the class of consistent kernel SSP estimators with the reproducing kernel structures adapted to the metrics in such the solution space. Next, the “model-free” variational analysis (VA)-based image enhancement approach and the “model-based” descriptive experiment design (DEED) regularization paradigm are unified into a new dynamic experiment design (DYED) regularization framework. Application of the proposed DYED framework to the adaptive array radar/SAR imaging problem leads to a class of two-level (DEED-VA) regularized SSP reconstruction techniques that aggregate the kernel adaptive anisotropic windowing with the projections onto convex sets to enforce the consistency and robustness of the overall iterative SSP estimators. We also show how the proposed DYED regularization method may be considered as a generalization of the MVDR, APES and other high-resolution nonparametric adaptive radar sensing techniques. A family of the DYED-related algorithms is constructed and their effectiveness is finally illustrated via numerical simulations.
Recent Advances in Neural Recording Microsystems
Authors: Gosselin Benoit
Published: April 2011

The accelerating pace of research in neuroscience has created a considerable demand for neural interfacing microsystems capable of monitoring the activity of large groups of neurons. These emerging tools have revealed a tremendous potential for the advancement of knowledge in brain research and for the development of useful clinical applications. They can extract the relevant control signals directly from the brain enabling individuals with severe disabilities to communicate their intentions to other devices, like computers or various prostheses. Such microsystems are self-contained devices composed of a neural probe attached with an integrated circuit for extracting neural signals from multiple channels, and transferring the data outside the body. The greatest challenge facing development of such emerging devices into viable clinical systems involves addressing their small form factor and low-power consumption constraints, while providing superior resolution. In this paper, we survey the recent progress in the design and the implementation of multi-channel neural recording Microsystems, with particular emphasis on the design of recording and telemetry electronics. An overview of the numerous neural signal modalities is given and the existing microsystem topologies are covered. We present energy-efficient sensory circuits to retrieve weak signals from neural probes and we compare them. We cover data management and smart power scheduling approaches, and we review advances in low-power telemetry. Finally, we conclude by summarizing the remaining challenges and by highlighting the emerging trends in the field.
Fluorescent Sensing of Chlorophenols in Water Using an Azo Dye Modified β-Cyclodextrin Polymer
Authors: Ncube Phendukani , Krause Rui W. , Mamba Bhekie B.
Published: April 2011

A water soluble azo dye modified β-cyclodextrin polymer 4 was synthesized and used as a chemosensor for the detection of chlorinated phenols, model chlorinated by-products (CBPs) of water treatment for drinking purposes. The characterization of the intermediates and the azo dye modified β-CD polymer was done by UV/Vis Spectrophotometry, FT-IR and 1H-NMR spectroscopies. The chlorophenols were capable of quenching the fluorescence of the polymer. The polymer showed greater sensitivity towards 2,4-dichlorophenol, with a sensitivity factor of 0.35 compared to 0.05 and 0.12 for phenol and 4-chlorophenol, respectively. The stability constants (Ks) of the pollutants were also determined by the Benesi-Hildebrand method to be 2.104 × 103 M−1 for 2,4-dichlorophenol and 1.120 × 102 M−1 for 4-chlorophenol.
Sustainable Water Resource Management and Participatory System Dynamics. Case Study: Developing the Palouse Basin Participatory Model
Authors: Beall Allyson , Fiedler Fritz , Boll Jan , Cosens Barbara
Published: April 2011

The complexity of explaining highly scientific information and juggling a plethora of social values is leading agencies and communities such as those in the Palouse Basin to explore the use of participatory modeling processes using system dynamics. Participatory system dynamics as a methodology creates a transparent nexus of science, policy options, social concerns and local knowledge that enhances discussion of issues surrounding the use of natural resources. The process of developing a systems model uses the tenets of scientific theory, hypothesis testing and clear statements of assumptions. A unique aspect of the Palouse basin project is the use of system dynamics to describe ground water dynamics in a sole source confined aquifer system. There are, as of yet, no standards for analyzing participatory modeling projects, therefore, we use case study analysis to describe the process, insights and qualitative measurements of success.
Quantum Theory and Probability Theory: Their Relationship and Origin in Symmetry
Authors: Goyal Philip , Knuth Kevin H.
Published: April 2011

Quantum theory is a probabilistic calculus that enables the calculation of the probabilities of the possible outcomes of a measurement performed on a physical system. But what is the relationship between this probabilistic calculus and probability theory itself? Is quantum theory compatible with probability theory? If so, does it extend or generalize probability theory? In this paper, we answer these questions, and precisely determine the relationship between quantum theory and probability theory, by explicitly deriving both theories from first principles. In both cases, the derivation depends upon identifying and harnessing the appropriate symmetries that are operative in each domain. We prove, for example, that quantum theory is compatible with probability theory by explicitly deriving quantum theory on the assumption that probability theory is generally valid.
Effects of Formal and Informal Support Structures on the Motivation of Native American Students in Nursing
Authors: Metz Anneke M. , Cech Erin A. , Babcock Tracy , Smith Jessi L.
Published: April 2011

Native Americans have traditionally been underrepresented in nursing. The authors surveyed 19 undergraduate nursing students participating in a university sponsored Native American nursing student support program and examined which social support factors influenced the students’ success. Using validated quantitative measures from social psychology, the authors found that overall perceived social support, as well as support from the university sponsored program, positively influenced Native American students’ identification with nursing, their interest in nursing, their perception of the value of nursing, and their motivation to continue pursuing nursing as a career. Conversely, perceptions of unfairness due to racial bias within the major negatively affected students’ perception of the value of nursing, as well as their motivation to pursue a nursing career.
Using Distance Technology to Learn Across Borders: A Virtual Travel Course in Nursing
Authors: Gallagher-Lepak Susan , Block Derryl , Rojas Yrene Esperanza Urbina , Birkholz Lorri , Melgar Morán Carlos Christian
Published: April 2011

A 6-week online course was developed and delivered to nursing students and instructors at universities in two countries. The course exposed students and faculty to nursing and health concerns in both countries. All course communications were conducted in both English and Spanish, with support from online translation software as needed. Course content covered professional nursing, global health issues, and nursing interventions used with clinical problems. Although students were initially intimidated by the course language requirements, students valued the opportunity to learn about cultural and health issues. Faculty experienced a learning curve as well and enjoyed this international experience.
Effects of Concept Map Teaching on Students’ Critical Thinking and Approach to Learning and Studying
Authors: Chen Shiah-Lian , Liang Tienli , Lee Mei-Li , Liao I-Chen
Published: April 2011

The purpose of this study was to explore the effects of concept mapping in developing critical thinking ability and approach to learning and studying. A quasi-experimental study design with a purposive sample was drawn from a group of nursing students enrolled in a medical-surgical nursing course in central Taiwan. Students in the experimental group were taught to use concept mapping in their learning. Students in the control group were taught by means of traditional lectures. After the intervention, the experimental group had better overall critical thinking scores than did the control group, although the difference was not statistically significant. After controlling for the effects of age and the pretest score on critical thinking using analysis of covariance, the experimental group had significantly higher adjusted mean scores on inference and overall critical thinking compared with the control group. Concept mapping is an effective tool for improving students’ ability to think critically.
Leisure and Social Participation and Health-Related Quality of Life in Caregivers of Children with Autism
Authors: Rizk Sabrin , Pizur-Barnekow Kris , Darragh Amy R.
Published: April 2011

The objective of this cross-sectional study was to compare health-related quality of life (HRQoL) in mothers of children with autism spectrum disorders (ASD) with a norm-based sample. An additional aim was to examine the relationship between engagement in leisure and social occupations and HRQoL in mothers of children with ASD. Thirty-three mothers of children with ASD, between 27 and 49 years of age, participated. Study participants between the ages of 35 and 44 years reported poorer mental HRQoL than a national sample, but comparable physical HRQoL. Findings also revealed inverse correlations between participation in hobbies and community/education activities and physical HRQoL (e.g., participation in hobbies and community education/activities increased as physical HRQoL decreased). The mental HRQoL of caregivers of children with ASD is lower than that of the general population, suggesting the need for occupational therapists to include psychosocial screening and referral for this group in their practice. In addition, results suggest that the relation between participation and health is complex, warranting further investigation.
Nursing Diagnoses, Interventions, and Patient Outcomes for Hospitalized Older Adults with Pneumonia
Authors: Head Barbara J. , Scherb Cindy A. , Reed David , Conley Deborah Marks , Weinberg Barbara , Kozel Marie , Gillette Susan , Clarke Mary , Moorhead Sue
Published: April 2011

A study was conducted by academic and community hospital partners with clinical information systems that included the standardized nursing language classifications of the North American Nursing Diagnosis Association International (NANDA-I), Nursing Interventions Classification (NIC), and Nursing Outcomes Classification (NOC). The aim of the study was to determine the frequency of NANDA-I, NIC, and NOC (NNN) terms documented for older adults with pneumonia who were discharged from three hospitals during a 1-year period. NNN terms were ranked according to frequency for each hospital, and then the rankings were compared with previous studies. Similarity was greater across hospitals in rankings of NANDA-I and NOC terms than in rankings of NIC terms. NANDA-I and NIC terms are influenced by reimbursement and regulatory factors as well as patient condition. The 10 most frequent NNN terms for each hospital accounted for only a small to moderate percentage of the terms selected.
Esferodóridos (Polychaeta, Sphaerodoridae) del margen continental del NW de la península Ibérica y primer registro de Sphaerodoropsis sibuetae y S. amoureuxi tras su descripción original
Authors: Moreira J. , Lucas Y. , Parapar J.
Published: April 2011

El conocimiento sobre los Sphaerodoridae (Annelida, Polychaeta) es todavía insuficiente en muchas partes del planeta, incluyendo los fondos del sistema marino profundo de la península Ibérica. El estudio de las muestras recogidas entre 100 y 2000 m de profundidad durante varias campañas oceanográficas en el Margen Continental de Galicia (NW península Ibérica) entre 2003 y 2009 ha mostrado la presencia de seis especies de poliquetos pertenecientes a la familia Sphaerodoridae. Dos de estas especies son mencionadas por primera vez desde su descripción original: Sphaerodoropsis sibuetae Desbruyères, 1980 y S. amoureuxi Aguirrezabalaga & Ceberio, 2005; estos registros extienden la distribución conocida de ambas especies hacia el Oeste en el Océano Atlántico. Asimismo, se mencionan todas las especies encontradas aportando datos sobre su distribución y ecología, y se incluye una clave con todas las especies conocidas de la península Ibérica.
Lipopolysaccharide Animal Models for Parkinson's Disease
Authors: Liu Mei , Bing Guoying
Published: April 2011

Lipopolysaccharide (LPS), an endotoxin from Gram-negative bacteria, acts as a potent stimulator of microglia and has been used to study the inflammatory process in the pathogenesis of Parkinson's disease (PD) and anti-inflammatory therapy for PD treatment. Here, we review the growing body of literature on both in vitro and in vivo LPS PD models. Primary cell cultures from mesencephalic tissue were exposed to LPS in vitro; LPS was stereotaxically injected into the substantia nigra, striatum, or globus pallidus of brain or injected into the peritoneal cavity of the animal in vivo. In conclusion, the LPS PD models are summarized as (1) local and direct LPS treatment and (2) systemic LPS treatment. Mechanisms underlying the PD models are investigated and indicated that LPS induces microglial activation to release a variety of neurotoxic factors, and damaged neurons may trigger reactive microgliosis, which lead to progressive dopaminergic neurodegeneration.
Evasion of Host Defence byLeishmania donovani: Subversion of Signaling Pathways
Authors: Shadab Md. , Ali Nahid
Published: April 2011

Protozoan parasites of the genusLeishmaniaare responsible for causing a variety of human diseases known as leishmaniasis, which range from self-healing skin lesions to severe infection of visceral organs that are often fatal if left untreated.Leishmania donovani(L. donovani), the causative agent of visceral leishmaniasis, exemplifys a devious organism that has developed the ability to invade and replicate within host macrophage. In fact, the parasite has evolved strategies to interfere with a broad range of signaling processes in macrophage that includes Protein Kinase C, the JAK2/STAT1 cascade, and the MAP Kinase pathway. This paper focuses on howL. donovanimodulates these signaling pathways that favour its survival and persistence in host cells.
Myeloid Sarcoma: The Clinician's Point of View
Authors: Malagola M. , Tiribelli M. , Russo D. , Candoni A. , Visani G. , Isidori A.
Published: April 2011

Myeloid Sarcoma may occur in patients with an acute or chronic myeloproliferative disorder as well as de novo, with no apparent sign or symptom of concomitant haematological disease. The patients are preferentially young male and the site of disease localization may vary from central nervous system to pleura and thorax, with a common involvement of the reticuloendothelial system. The disease often shows chromosomal rearrangements, involving chromosomes 7, 8 and 3 and sometimes a complex karyotype (more than 3 abnormalities) is detected at diagnosis. The prognosis of this disease is dismal and only high-dose chemotherapy with autologous or allogeneic stem cells transplantation (auto or allo-SCT) may be potentially curative. In the absence of definitive elements that can define the prognosis of extra-medullary localization of “standard risk” AML, Clinicians should pursue the collection of data from different Centres and design of homogeneous treatment strategies, that could integrate standard chemotherapy with specific approaches, such as radiotherapy, transplant procedures or, in selected cases (such as those displaying molecular abnormalities involving protein tyrosine-kinases), molecularly targeted therapies.
Evaluation of Comprehensive 2D Gas Chromatography-Time-of-Flight Mass Spectrometry for 209 Chlorinated Biphenyl Congeners in Two Chromatographic Runs
Authors: Osemwengie L. I. , Sovocool G. W.
Published: April 2011

This research evaluates a recently developed comprehensive two-dimensional gas chromatography 2D GC coupled with a time-of-flight (TOF) mass spectrometer for the potential separation of 209 PCB congeners, using a sequence of 1D and 2D chromatographic modes. In two consecutive chromatographic runs, using a 40 m, Rtx-PCB column, and a 1 m DB-17 column, connected in series, 196 PCB congeners are distinguished, including 43 of the 46 pentachlorobiphenyl isomers. Some of the chlorinated biphenyls that could not be resolved chromatographically are resolved with the use of the “ortho effect,” which distinguishes PCB isomers having 2,2′-; and 2,2′6- chlorine substitution from those isomers without these substitutions. The result of this work falls short of our goal of separating all 209 PCB congeners but still provides investigators with a new tool for a better front-end separation of PCB-specific congeners, and potentially, for use in acquisition of more accurate data.
Giant Cell Myocarditis with Incessant Ventricular Arrhythmias Treated Successfully with Methylprednisolone and Rat Antithymocyte Globulin
Authors: Baig Mudassar , Hatrick Rob
Published: April 2011

Giant cell myocarditis is an aggressive form of this condition that is typically progressive and unresponsive to usual medical treatment. Here, we describe a 34-year-old patient presenting with incessant ventricular arrhythmias with hemodynamic compromise who required prolonged support in intensive care with an intra-aortic balloon pump (IABP). His Coronary arteries were normal and LV endomyocardial biopsy revealed myocyte necrosis with inflammatory infiltrate of lymphocytes, eosinophils, and giant cells suggestive of giant cell myocarditis. He was successfully treated with pulsed intravenous methylprednisolone and rat antithymocyte globulin (RATG). Despite a good functional cardiac recovery, some months later he developed a fluctuant neck swelling which fine needle aspiration confirmed as tuberculosis.
Hemotoxicity Induced by Chronic Chlorpyrifos Exposure in Wistar Rats: Mitigating Effect of Vitamin C
Authors: Ambali Suleiman F. , Ayo Joseph O. , Esievo King A. N. , Ojo Samuel A.
Published: April 2011

The study evaluated the ameliorative effect of vitamin C on chronic chlorpyrifos-induced hematological alterations in Wistar rats. Twenty adult male rats divided into 4 groups of 5 animals each were exposed to the following regimens: group I (S/oil) was administered soya oil (2 mL/kg b.w.), while group II (VC) was given vitamin C (100 mg/kg b.w.); group III was dosed with CPF (10.6 mg/kg b.w.); group IV was pretreated with vitamin C (100 mg/kg) and then exposed to CPF (10.6 mg/kg b.w.), 30 minutes later. The regimens were administered by oral gavage once daily for a period of 17 weeks. Blood samples collected at the end of the study revealed reduction in the levels of pack cell volume, hemoglobin, red blood cells, leukocytes (attributed to neutropenia, lymphopenia, and monocytopenia), and platelets in the CPF group, which were ameliorated in the vitamin C- pretreated group. The elevated values of malonaldehyde, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and neutrophil/lymphocyte ratio in the CPF group were restored in those pretreated with vitamin C. The study has shown that chronic CPF-induced adversity on hematological parameters of Wistar rats was mitigated by pretreatment with vitamin C.
Unilateral Optic Neuritis as a Presentation of Neurobrucellosis
Authors: Marques Rita , Martins Cristina , Machado Inês , Monteiro José Paulo , Campos Nuno , Calhau Paulo
Published: April 2011

Neurobrucellosis manifesting as optic neuritis is a rare disease in childhood. We report a case of neurobrucellosis in a 11 year old girl leading to visual impairment and headache. Physical examination revealed mild oedema of right tibiotarsic joint and optic neuritis. Investigations showed CSF pleocytosis and a Brucella serum agglutination titer of 1/640. Complete reversal of the symptoms was observed after appropriate antibiotic treatment. To our knowledge only four cases of neurobrucellosis manifesting with visual impairment in childhood are previously reported in literature.
The Characterization of Cell Line CRL-2335 as a Basal-Like Breast Carcinoma Model
Authors: Neves Lori A. Hill , Ingram Lishann , Davis Melissa B.
Published: April 2011

Basal-like breast cancer has been reported to be the most aggressive and deadly carcinoma sub-type. Patients diagnosed with this subtype have a less than 50% five-year survival. In addition, many studies have reported that this sub-type is more prevalent in specific ethnic groups and is believed to be a key factor that drives certain ethnic disparities in mortality. In order to effectively study this sub-type and determine unique gene expression and biochemical pathways which sustain this cancer's growth, we sought to identify human breast cancer cell lines that represent a model for the basal-like subtype. Here, we report our findings which indicate the African American cell line CRL-2335 is a true representative of basal-like breast carcinoma.
Quadripolar Left Ventricular Lead in a Patient with CRT-D does Not Overcome Phrenic Nerve Stimulation
Authors: Parahuleva Mariana S. , Chasan Ritvan , Soydan Nedim , Abdallah Yasser , Neuhof Christiane , Tillmanns Harald , Erdogan Ali
Published: April 2011

Effective cardiac resynchronization therapy (CRT) requires an accurate atrio-biventricular pacing system. The innovative Quartet lead is a quadripolar, over-the-wire left ventricular lead with four electrodes and has recently been designed to provide more options and greater control in pacing vector selection. A lead with multiple pacing electrodes is a potential alternative to physical adjustment of the lead and may help to overcome high thresholds and phrenic nerve stimulation (PNS).
Measurements of atmospheric mercury in Shanghai during September 2009
Authors: Friedli H. R. , Arellano A. F. , Geng F. , Cai C. , Pan L.
Published: April 2011

Abstract. We report on total gaseous mercury (TGM) measurements made in Pudong, Shanghai in August/September 2009. The average TGM was 2.7 ± 1.7 ng m−3. This represents about 90% of the total atmospheric mercury. This is an underestimate for an annual-mean concentration because the meteorology in September favored predominantly easterly oceanic air, replaced in other seasons by airflow from industrial areas. The observed TGM follows a pattern seen in other cities around the world: a background elevated over mean hemispheric background (1.5 ng m−3), and pollution plumes of different magnitude and duration, interspersed with very sharp spikes of high concentration (60 ng m−3). The September 2009 Shanghai measurements are lower than those reported for most other Chinese cities and Mexico City, and similar to concentrations found in some Asian and in North American cities. Such comparisons are tenuous because of differences in season and year of the respective measurements. Our results should not be used for regulatory purposes. We find that the observed TGM are most likely coming from coal fired power plants, smelters and industrial sources, based on its high correlation with NOx, SO2, CO and wind directions.