bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of “TCM + Smart Elderly Care” in the Medical-Nursing Care Integration Service System
Authors: Wang Xuanxuan , Shi Huaiying , Lu Guo , Huang Zhiping , Zhang Yichen , Lao Yumei , Li Guangjie , Gong Xun , Wang Ping , Wang Xing , Zhang Yidan
Published: October 2022

As a country with the fastest population aging rate in the world, China’s existing elderly care and medical resources are not enough to meet the growing and complex needs of elderly care and health services. The Chinese government is actively promoting the integrated development of medical and elderly care institutions, but it has yet to give full play to the inexpensive and existing advantages of traditional Chinese medicine in the treatment of diseases and rehabilitation of the elderly, by sorting out the advantages of “TCM + intelligent pension” in medical expenses, pension costs, disease prevention, intelligent pension services, and so on. In view of the current problems existing in China’s pension, this paper mainly proposes to solve the pension dilemma in China from several aspects: improving the supervision and evaluation system, increasing capital investment, building a unified digital medical and pension service platform, and strengthening the talent training in the field of traditional Chinese medicine integration. The application of the “TCM + smart elderly care” system into the medical and elderly care service system is promoted to effectively improve the operation efficiency of the whole medical and elderly care service system and the satisfaction of elderly families.
The Efficacy and Safety of the Zhuyun Formula and Auricular Acupressure for the Infertile Women with Recurrent Implantation Failure: A Randomized Controlled Trial
Authors: Zhou Hang , Zheng Xiaoyan , Xia Wanting , Ma Qianhong , Li Jinmei , Zeng Qian , Huang Jinzhu
Published: October 2022

Background. Recurrent implantation failure (RIF), a clinical disorder characterized by failure to achieve pregnancy after repeated (≥3) embryo transfer, is a challenge for reproductive demands worldwide. In our preliminary work, the Zhuyun formula (ZYF) with auricular acupressure, a complementary and alternative medicine (CAM) with a small sample size for RIF, can improve the clinical pregnancy rate (41.2% vs. 26.7%, treatment group vs. control group,

p
<
0.05

). Based on the toxicological and pregnancy-related pharmacological analysis of ZYF for RIF, the T-cell receptor signaling pathway might be involved in the pharmacological activity. This study aimed at evaluating the efficacy and safety of the CAM therapy according to pregnancy outcomes and maternal and child health and investigating the changes of T-helper (Th) cells in the peripheral blood of unexplained RIF women. Materials and Methods. We conducted a prospective, two-arms, randomized, nonblinded study. All eligible women were randomly assigned to the treatment group (TG) and the control group (CG) according to a computer-generated randomization list in sealed opaque envelopes. Blood samples were collected from the two groups, and serum Th1, Th2, and Treg were detected by flow cytometry. The cytokines were detected by an enzyme-linked immunosorbent assay (ELISA). The TG was administrated with ZYF and auricular acupressure for three months before ovarian stimulation, while the control group was on a waiting list for the same period. The primary outcome was CPR. The second outcomes were the serum levels of immune parameters. For the safety evaluation, the perinatal outcomes of maternal and child were obtained by follow-up. Post-hoc sensitivity analyses were performed to assess the effect of missing data. Results. One hundred and twenty-three women were randomized into the TG (n = 62) and CG (n = 61). The CPR was increased significantly in the TG (45.2%) than CG (26.2%) (p = 0.029). Twenty blood samples were collected, and the Th2/Th1 and Treg expression level was significantly higher in the TG than in the CG. IL-2, IL-10, and Foxp3 were higher significantly in the TG than in the CG. The maternal and child perinatal outcomes were not significantly different between the two groups. Conclusions. The ZYF with auricular acupressure was effective and safe in improving the pregnancy outcomes of RIF. It might be related to balancing the level of cytokines related to the immune tolerance of the maternal-fetal interface to protect the embryo from the maternal immune system. Clinical Trial Registration: Clinical Trial Registry; date: 14/Dec/2013; no. NCT03078205.
[Retracted] Influence of Taijiquan Exercise on Mentality and Emotion Regulation by Intelligent Medical Big Data Analysis
Authors: Han Shuaiju , Hu Shaohui , Li Kang
Published: October 2022

Taijiquan training emphasizes the relaxation of the mind and the body, pay attention to maintain the peace of mind, and minimize the impact of external interference on the body so as to make the mind more comfortable. This study mainly explores the influence of Taijiquan practice on emotion regulation based on intelligent medical health big data analysis. The significance of Taijiquan in developing and improving the positive emotions of middle‐aged people and maintaining physical and mental health is expounded. There are two methods of data collection: full collection and incremental collection. In this study, when the psychological testing equipment was launched, a full amount of historical data was collected; after the psychological testing equipment was online, the collection method was generally carried out in the way of incremental collection. The subjects exercised Taijiquan three times a week, one hour each time, and the exercise content was the 24‐style Taijiquan designated by the workstation. At the same time, the subjects were asked not to engage in other regular physical exercise projects in their spare time. By longitudinal tracking and comparison of the Taijiquan intervention group after participating in the Taijiquan exercise intervention, the differences in the state of mind and emotion regulation strategies, and 12 subjects were selected voluntarily to participate in the emotional Stroop (the color words used in the classic Stroop paradigm were replaced with emotional and nonemotional words written in different colors, and the subjects were still tasked with responding to colors) experimental paradigm. In this paper, the moderate‐intensity Taijiquan project is selected, which is in line with the effective value threshold theory of exercise load. It studies the effects of exercise on the body shape, cardiopulmonary function, flexibility, and balance ability of the body according to the metabolism theory and the movement balance theory of the human body adapting to the environment. Before the experiment, there was no significant difference between the Taijiquan training group and the control group, but after the experiment, there was a significant difference between the Taijiquan training group and the control group (P<0.05). Taijiquan has a significant effect on improving students’ body shape, cardiopulmonary function, flexibility, balance, and mood.
Node Location Method of Ecological Environment Monitoring Network Based on Zigbee
Authors: Li Linsen
Published: October 2022

In view of the poor initialization performance of ecological environment monitoring network node location, a method of ecological environment monitoring network node location based on Zigbee is proposed. The node data collection model of ecological environment monitoring network is built based on Zigbee, and the performance is stable, which is more suitable for the node location of the ecological environment monitoring network; it is hoped that this study can provide reliable value reference and help for the future ecological studies. Through the installation of different types of sensors, the data of the ecological environment monitoring network nodes are automatically collected and sent to the server. The static weight coefficient of the collected data of the ecological environment monitoring network nodes is modified. According to the modified results, the ecological environment monitoring network is modified by DV‐HOP positioning algorithm. The nodes of the ecological environment monitoring network are located by the three‐way positioning method. The experimental results show that the initialization performance of this method is better, the accuracy is about 98%, and it is stable. It is more suitable for the node location of ecological environment monitoring network, which mainly includes ZigBee wireless sensor network module, embedded ARM, and Linux.
Construction and Application of Seismic Real-Time Data Analysis Model Based on GPS Monitoring
Authors: Yan Wei
Published: October 2022

GPS (global position system) monitoring has great theoretical and practical significance for understanding regional crustal deformation and disaster prevention and mitigation. Based on the GPS system differential model, this paper builds a seismic real-time data analysis model and discusses and analyzes the GNSS (global navigation satellite system) single-system single-difference model, single-system double-difference model, and dual-system double-difference model. When the model is processing the data of the GNSS dual-satellite positioning system, the proposed short-baseline ambiguity solution is studied to a certain extent and solves the problems that other common methods can only detect large cycle slips or require dual-frequency data. During the simulation process, through the study of the GPS coordinate time series, the stability of the site, the periodic change of the site, the movement law of the plate, and other information can be analyzed. The combined method is used to estimate the station noise, coordinate time series parameters, and the station velocity, and then the influence of the common mode error on the station noise, parameter estimation, and station velocity estimation is studied. The experimental results show that the average magnitude of white noise and colored noise decreases by 31.61% and 42.28%, respectively, which effectively reduces the uncertainty of parameter estimation and the uncertainty of station velocity estimation.
Study on the Mechanical Mechanism and Prevention Method of Casing Moving up: Taking the Jidong Oilfield as an Example
Authors: Zhou Yan , Song Wei , Zhao Wanchun , Shen Yuanyuan , Wang Lingling , Chen Chunlai , Zhu Kuanliang , Pan Junying
Published: October 2022

In the production process of oilfields, the proportion of oil and water wells scrapped or overhauled by casing moving up continues to increase, especially in some onshore oilfields near the coast, which is more prominent. In order to clarify the causes of casing moving up and propose the treatment method of casing moving up, this paper first establishes the buckling deformation model of groundwater extraction-induced surface settlement and the buckling deformation model of casing on the surface settlement stress field and then combines the relationship between the axial jackal force generated by the buckling deformation of the casing of different well structures and the jacking strength of the casing head to give the criterion for the casting of the casing moving up. Taking Jidong Oilfield as an example, the displacement of running-up wells on 50 casings was analyzed, and the identification compliance rate reached 85.68%, and the variation law of running-up on casing with time and casing structure was given, which provided scientific guidance for subsequent casing running-up protection. Based on the model, it is concluded that enhancing the casing structure, the length of the casing cementing and sealing section, and enhancing the casing prestress are helpful for preventing the casing from moving up.
Elevated MFG-E8 in CSF in the Early Stage Indicates Rapid Recovery of Mild Aneurysmal SAH Patients
Authors: Pang Cong , Peng Zheng , Li Xiaojian , Gao Yongyue , Liu Xunzhi , Wang Han , Lu Yue , Zhuang Zong , Zhang Qingrong , Li Wei , Hang Chunhua
Published: October 2022

Background. Aneurysmal subarachnoid hemorrhage (aSAH) can impair blood perfusion in brain tissue and cause adverse effects. Microglia, which are the inherent immune cells of the brain, significantly activate and play a role in phagocytosis, anti-inflammatory, proinflammatory, and damage repair in this process. Milk fat globule epidermal growth factor 8 (MFG-E8) is the bridging molecule of this process and mediates the activation and biological effects of microglia. Methods. We obtained cerebrospinal fluid (CSF) from patients with aSAH at various times (the third day, seventh day, and ninth day) as well as from patients in the control cohort. MFG-E8 protein levels in CSF were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we evaluated the GCS and GOS of aSAH patients on admission and on the third day, seventh day, ninth day, and at discharge. Then, we analyzed the association between the levels of MFG-E8 and the changes in GCS and GOS. Results. MFG-E8 expression rose in the early stage on the third day and reached equilibrium around day 7 and day 9. The levels of MFG-E8 on the third day were associated with the change in GOS on the seventh day (

r
=
0.644

,

p
=
0.018

) and ninth day (

r
=
0.572

,

p
=
0.041

) compared with admission but were not correlated with the change on day 3 or at discharge. The levels of MFG-E8 were not correlated with any change in GCS. Conclusions. We found that aSAH resulted in an upregulation of MFG-E8 in CSF. Moreover, high MFG-E8 levels in the early stage indicated a rapid recovery of mild aSAH patients.
Correlation between Ferroptosis-Related Gene Signature and Immune Landscape, Prognosis in Breast Cancer
Authors: Zhu Jiahao , Chen Qingqing , Gu Ke , Meng You , Ji Shengjun , Zhao Yutian , Yang Bo
Published: October 2022

Breast cancer (BC) is the most commonly diagnosed cancer and second leading cause of cancer-related death in women worldwide. Ferroptosis, an iron-dependent newly discovered mode of cell death, can be induced by lenaltinib and plays an important role in the biological behaviors of BC. Therefore, the prognostic value of ferroptosis-related genes (FRGs) in BC warrants further investigation. FRG expression profiles and clinical data were downloaded from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO). Immune-related pathways were found in the functional analysis. Significant differences in enrichment scores for immune cells were observed. Some patients from TCGA-BRCA were included as the training cohort. A six-gene prediction signature was constructed with the least absolute shrinkage and selection operator Cox regression. This model was validated in the rest of the TCGA-BRCA and GEO cohort. The expressions of the six FRGs were verified with real-time quantitative polymerase chain reaction and immunohistochemistry in the Human Protein Atlas. Relapse or metastasis was more likely in the high-risk group. Risk score was an independent predictor of disease-free survival. Collectively, the ferroptosis-related risk model established in this study may serve as an effective tool to predict the prognosis in BC.
Extended Newton-type Method for Nonsmooth Generalized Equation under

n
,
α

-point-based Approximation
Authors: Khaton M. Z. , Rashid M. H.
Published: October 2022

Let

X

and

Y

be Banach spaces and

Ω
⊆
X

. Let

f
:
Ω
⟶
Y

be a single valued function which is nonsmooth. Suppose that

F
:
X⇉

2

Y

is a set-valued mapping which has closed graph. In the present paper, we study the extended Newton-type method for solving the nonsmooth generalized equation

0
∈
f

x

+
F

x

and analyze its semilocal and local convergence under the conditions that

f
+
F

−
1

is Lipschitz-like and

f

admits a certain type of approximation which generalizes the concept of point-based approximation so-called

n
,
α

-point-based approximation. Applications of

n
,
α

-point-based approximation are provided for smooth functions in the cases

n
=
1

and

n
=
2

as well as for normal maps. In particular, when

0
<
α
<
1

and the derivative of

f

, denoted

∇
f

, is

ℓ
,
α

-Hölder continuous, we have shown that

f

admits

1
,
α

-point-based approximation for

n
=
1

while

f

admits

2
,
α

-point-based approximation for

n
=
2

, when

0
<
α
<
1

and the second derivative of

f

, denoted

∇

2

f

, is

K
,
α

-Hölder. Moreover, we have constructed an

n
,
α

-point-based approximation for the normal maps

f

C

+
F

when

f

has an

n
,
α

-point-based approximation. Finally, a numerical experiment is provided to validate the theoretical result of this study.
Multiscale Investigations of Overtopping Erosion in Reinforced Tailings Dam Induced by Mud-Water Mixture Overflow
Authors: Chen Meibao , Jing Xiaofei , Liu Xiaohua , Huang Xuewei , Nie Wen
Published: October 2022

The overtopping failure that occurred to the reinforced tailings dam has led to devastating catastrophes, including severe environmental disasters as well as tragedy in terms of any loss of properties and life. To study the feature of overtopping erosion for the reinforced fine-grained tailings dam induced by mud-water mixture overflow, the red mud concentration of overflow, the erosion depths and erosion rate have been consistently detected and recorded by a series of physical model tests. This investigation conclusively showed that the red mud concentration for flooding over the dam slope had a nonlinear trend of first becoming larger, subsequently decreasing with the time going on in the process of tailing dam overtopping; the maximum red mud concentration achieved 19.0%. The erosion on the dam slope was shaped like a trumpet. The red mud concentration of the mud-water mixture flow affected the erosion depth significantly. The hydraulic tests simulating erosion behaviour in the reinforced fine-grained tailings were carried out with different red mud concentrations (0%, 10%, and 20%). For the mud-water mixture flow in a red mud concentration of 20%, the final erosion depth exhibited a ladder shape from the front to the trailing end (dam toe) of the tailings sample. The final erosion depths were 1.56 cm, 0.9 cm, and 0.55 cm at the front, middle, and trailing end, respectively. With the red mud concentration decreasing, the final erosion depth at the front of the tailings sample reduced significantly while increasing at the trailing end. This study brought more scientific insights into the overtopping erosion process to the reinforced fine-grained tailings dam triggered by the mud-water mixture flow at multiscale.
[Retracted] Characterization of Clinical Symptoms of Cervical Spondylotic Myelopathy Based on DKI Imaging Parameter Ratios
Authors: Tian Xiaonan , Zhang Li , Liu Hongran , Zhang Xuesong , Sun Yingcai , Zhang Yujin
Published: October 2022

In recent years, people’s living conditions have significantly improved, and their lifestyles have been diversified. However, the incidence of various diseases has also increased with it, including cervical spondylotic myelopathy (CSM). Spinal cord cervical spondylosis is one of the spinal cord compression disorders that can be severely disabling and accounts for 10% to 15% of all cervical spondylosis. In this paper, DKI image processing technology is used to study the symptoms of cervical spondylosis, which is helpful to help them explore the symptoms and causes. The onset of cervical spondylosis has a longer period of time, and the period of conservative treatment will inevitably require a longer period of time. The clinical symptoms of cervical spinal cord compression are mainly pain, and after the cervical spinal cord nerve is compressed, ischemia and hypoxia will occur, the nerve sensitivity will increase, and the patient will have reflex neck muscle pain symptoms. This causes degeneration of the patient’s intervertebral disc tissue, degeneration of facet joints, ossification of the posterior longitudinal ligament, and formation of spurs on the posterior edge of the vertebral body. The condition will become more complicated, so it is very important to identify the characteristics of the clinical symptoms of cervical spondylotic myelopathy to help patients with cervical spondylosis recover. This paper identifies the clinical symptoms of cervical spondylosis based on the parameter ratio method of Diffusional Kurtosis Imaging (DKI). The current state of diffusion kurtosis imaging and the treatment of cervical spondylosis and the treatment operation are introduced, and the image enhancement technology in medical imaging is used to analyze the disc herniation of each segment in the overflexion, neutral and hyperextension positions. After comparing the FA, MK, and MD values in the spinal cord between the normal group and the patient group, the results showed that the FA, MD and MK values in the patient group were lower than those in the normal group, and the findings showed that the MD and MK values were positively correlated with the JOA score, reflecting that as the clinical symptoms of spinal cervical spondylosis worsened. The size and number of cervical intervertebral disc bulge on the hyperextension image is obvious and the number increases (especially the C4/5, C5/6, and C6/7 intervertebral discs with greater mobility), and the highest is 0.82 and 27%, respectively.
Appearance Design Method of Ceramic Art Based on VR Visualization Technology
Authors: Li Hui
Published: October 2022

In order to improve the effect of ceramic art appearance design, this work studies the appearance design method of ceramic art combined with VR visualization technology and builds an intelligent model. According to the needs of the industrial site and the requirements of the lighting system, the area type of the lighting area is determined in this study, and the light of the virtual ceramic product is irradiated on the target detection plane through the refraction of the lens. Based on the Lambertian luminescence characteristics of the point light source virtual ceramic products and the uniform distribution of illuminance on the lighting surface, a mathematical model of the lighting system is established. Taking the realization of a rectangular lighting area with high uniformity as the main goal, the mathematical model is used to construct a system of partial differential equations of free-form surfaces, and the effect of the method in this study is verified by combining with the simulation research. The experimental research shows that the appearance design system of ceramic art based on VR visualization technology proposed in this study has a good effect and can effectively improve the interaction effect of appearance design of ceramic art.
Applications of Artificial Intelligence Enhanced Drones in Distress Pavement, Pothole Detection, and Healthcare Monitoring with Service Delivery
Authors: Wang Yue , Ye Tian
Published: October 2022

Artificial Intelligence (AI) has fascinated the present study assigned to multiple areas such as distress detection on the pavement, pothole detection, and healthcare. The distress detection on pavement and roads and delivering healthcare and medical services need to be monitored through state-of-the-art technology, i.e., drone technology. Improvement in construction sites and healthcare delivery are of serious concern. Nowadays, computer vision techniques are commonly used in this area utilizing images and videos of construction sites. Due to confined data, researchers are using Unmanned Aerial Vehicles (UAVs) or Drone to get maximum information through 360° monitoring. This review article presents the useful monitoring techniques using AI-enabled drones for scholars around the world. In this comprehensive review, initially, the image acquisition equipment along with the perks and limitations has been presented. Second, the main constraints related to different computer vision techniques are highlighted for detecting distress in the pavement. Then, the possible research solution to some of the distress issues such that detection of pavement texture, cracks or potholes, joint faulting, temperature segregation, and rutting issues are predicted. Finally, the application of AI-enhanced drones in the healthcare field is elucidated which showed their significance. Moreover, in this research, the comparative image analysis of pavement and path hole detection was presented for the collection of detailed information and accurate detection. In the future, the work can also be enhanced to monitor the live pavement distress detection, especially for busy roads and highways. Moreover, an analysis to determine and reduce the costs in the healthcare sectors and organizations is required for future work.
Relationship between Postoperative Pain and Sociocultural Level in Major Orthopedic Surgery
Authors: Gouveia Bárbara , Fonseca Sara , Pozza Daniel Humberto , Xará Daniela , Sá Rodrigues André
Published: October 2022

Background. Total knee arthroplasty (TKA) and total hip arthroplasty (THA) are associated with moderate to severe postoperative pain (POP). POP is theoretically predictable and may be influenced by sociocultural differences. This study aimed to identify the relationship between POP and the sociocultural level of the patient undergoing THA or TKA. Methods. Prospective study, involving informed-consenting adults conducted through consulting patient’s clinical processes, preoperative and postoperative questionnaires. Demographic and anthropometric data, type of surgery, ASA classification, sociocultural level of the patient, and POP were assessed. Results. 95 patients, all Caucasian and natural from the north of the Portugal, were included. Younger women undergoing TKA reported higher levels of POP. In females, the ASA 3 physical condition was also associated with higher mean pain intensity. Patients with preoperative chronic pain, without depression diagnosis, and unsatisfied with the current profession showed higher levels of reported POP. Retirees, with lower school degree, reported higher levels of minimal pain. Conclusions. Job satisfaction, type of surgery, body mass index, presence of chronic pain, and the absence of depression were identified as the main predictors of pain after THA or TKA.
Network Pharmacology Analysis of Xuanfei Baidu Granule in the Treatment of Intestinal Flora Disorder
Authors: Miao Yaodong , Chen Rui , Zhang Zeyu , Liu Yaoyuan , Yang Fengwen , Zhang Junhua
Published: October 2022

Objective. This study is aimed at analyzing the molecular mechanism of Xuanfei Baidu Granule (XFBDG) in the treatment of intestinal flora disorder based on network pharmacology. Methods. The TCMSP database was used to obtain the active components and target proteins of XFBDG, while the GeneCards database was used to obtain the related proteins of intestinal flora disorder. The Rx64 4.0.2 software was used to analyze the GO functional enrichment and KEGG pathway enrichment of drug component target protein and disease-related protein to obtain the pathway-enriched proteins and screen the core proteins for topology analysis of the pathway target by using the STRING database and Cytoscape v3.8.2 software. The Cytoscape v3.8.2 software was used to analyze the relationship between each component and enriched protein, and the AutoDock Vina software was used for molecular docking of core proteins and components. Results. XFBDG contains 133 active components that can act on 249 proteins related to intestinal flora disorder. The effects include the following: (i) regulation of functions—the response to drug, cellular response to chemical stress, response to oxidative stress, and RNA polymerase II-specific DNA-binding transcription factor binding and (ii) regulation of signaling pathways such as the IL-17 signaling pathway, TNF signaling pathway, and Th17 cell differentiation pathway. The enriched core proteins in these pathways are IFNG, IL4, PTGS2, JUN, and IL1B that set in a higher level of binding with the corresponding drug components. Conclusion. XFBDG can act on IFNG, IL4, PTGS2, JUN, and IL1B proteins through its active components such as quercetin, luteolin, and kaempferol to regulate the IL-17, TNF, and Th17 cell differentiation pathways and further regulate the response to drug, cellular response to chemical stress, response to oxidative stress, and RNA polymerase II-specific DNA-binding transcription factor binding. In addition, owing to its antioxidant and anti-inflammatory properties and related immune responses, XFBDG can achieve a balance of intestinal flora and microbial metabolism.
Activating Transcription Factor 3 Based Early Alarm Model of Acute Kidney Injury after Cardiopulmonary Bypass in Adults
Authors: Wu Xiao-Yun , Jin Xiang-Lan , Liu Qiang , Qiu Feng , Zhou Jian
Published: October 2022

Acute kidney injury (AKI) is a common complication after cardiopulmonary bypass (CPB) for cardiac surgery, and there is no effective treatment. This study was aimed at constructing an early warning model of AKI after CPB in adults and investigating the performance of this model. Patients who underwent CPB in the Department of Cardiac Surgery, Shanghai Tenth People’s Hospital, from January 2018 to December 2019 were recruited into the present study. Blood and urine samples were collected preoperatively (0 h) and 2 h, 6 h, 12 h, 24 h, and 48 h after surgery, and the creatinine and activating transcription factor 3 (ATF3) were detected. According to the diagnostic criteria of AKI, patients were divided into the AKI group and the non-AKI group, and the risk factors for AKI after CPB were screened. The receiver operating characteristic (ROC) curve analysis was used to identify the optimal biomarkers for the establishment of early warning model of AKI after CPB. Finally, the performance of this model was further verified. A total of 83 patients were included in this study, 42 of whom developed AKI after surgery. After CPB, the serum and urine levels of creatinine and ATF3 increased to different degrees, and the increase in urine ATF3 was the most obvious in the AKI group. The area under ROC (AUC) of urine ATF3 at 12 h after surgery was 0.691 (95% CI: 0.576-0.807). When ATF3 was higher than 1216 pg/mL, the sensitivity and specificity of ATF3 in the diagnosis of AKI were 0.43 and 0.85, respectively. The height, conjugated bilirubin on the surgery day, urine ATF3 12 h after surgery, and serum creatinine 24 h after surgery were independent risk factors for postoperative AKI. Urine ATF3 and other factors were used to establish AKI warning model after CPB, which showed good fitting and accuracy. In conclusion, ATF3 is an early biomarker of post-CPB AKI. Addition of urine ATF3 to AKI risk factors can improve the accuracy of early AKI prediction.
Evaluation of Regional Economic Innovation Ability Based on Neural Network
Authors: Tingting Song , Jiaying Wang , Nan Feng
Published: October 2022

In order to further improve regional economic innovation capability and governance level and solve the problems of lack of attention to evaluation indicators in traditional evaluation methods of regional economic innovation capability and easy to be affected by subjective factors, an evaluation model based on neural network algorithm is proposed. Through re-analysis of regional economic innovation capability evaluation indexes, the model defines the most reasonable combination of characteristics by combining information gain characteristic selection strategy and finally builds a scientific evaluation index system. By testing the prediction accuracy of the experimental discovery model and evaluation index, the neural network model improves by 41% compared with the traditional subjective evaluation method, and the accuracy increases by 20% compared with the GA-BP neural network model. The experiment proves the stability and good convergence effect of the evaluation model.
Long Noncoding RNA LINC00909 Induces Epithelial-Mesenchymal Transition and Contributes to Osteosarcoma Tumorigenesis and Metastasis
Authors: Liu Wanshun , Zhang Qi , Shen Kai , Li Keran , Chang Jie , Li He , Duan Ao , Zhang Sheng , Huang Yumin
Published: October 2022

Background. Osteosarcoma (OS) is a malignant tumor that is highly metastatic with a high mortality rate. Although mounting evidence suggests that LINC00909 is strongly associated with the malignant progression of various tumors, the exact role of LINC00909 in OS remains unknown. Therefore, the current study was designed to investigate the relationship between LINC00909 and the malignant progression of OS. Methods. LINC00909 expression was measured in OS cell lines and clinical specimens using RT-qPCR assays. The effects of LINC00909 on OS proliferation, invasion, and migration were calculated both in vitro and in vivo. Apart from that, bioinformatics analyses, FISH, RIP, and luciferase reporter assays were carried out to investigate the downstream target of LINC00909. Rescue experiments were also conducted to investigate the potential mechanisms of action of competitive endogenous RNAs (ceRNAs). Results. In this study, we found that LINC00909 was highly expressed in OS cell lines and clinical specimens. In vivo and in vitro experiments demonstrated that LINC00909 induces epithelial-to-mesenchymal transition (EMT) and contributes to OS tumorigenesis and metastasis. FISH, RIP, and luciferase assays indicated that miR-875-5p is a direct target of LINC00909. Moreover, HOXD9 was validated as the downstream target of miR-875-5p in a luciferase reporter assay and western blotting experiments. Rescue experiments revealed that HOXD9 reversed the effect of LV-sh-LINC00909 on OS cells by positively regulating the PI3K/AKT/mTOR signaling pathway. Conclusion. Collectively, LINC00909 induces EMT and contributes to OS tumorigenesis and metastasis through the PI3K/AKT/mTOR pathway by binding to miR-875-5p to upregulate HOXD9 expression. Targeting the LINC00909/miR-875-5p/HOXD9 axis may have potential in treating OS.
Investigation of the Clinical Effect of New Shoulder Joint Abduction Frame in Humeral Fracture Patients after Arthroscopic Shoulder Surgery
Authors: Yu Guiyang , Yu Meining , Liu Shan , Xue Hui , Sun Yuehua
Published: October 2022

Objective. This work is organized to analyze the clinical effects of new shoulder joint abduction frame on the bone metabolic markers, shoulder joint function, and visual analogue scale (VAS) of humeral fracture patients undergoing arthroscopic surgery. Methods. 118 patients with humeral fracture who planned to undergo shoulder surgery in our hospital from November 2018 to June 2021 were selected as the study objects and were divided into two groups according to the random number method, with 59 patients in each group. The patients in the two groups were subjected to arthroscopic shoulder surgery. New shoulder joint abduction frame was used for shoulder joint fixation in the abduction frame group, and sling was used for shoulder joint fixation in the sling group after surgery. The duration of fixation was 4-6 weeks. Finally, the prognostic indicators, complications, and serum bone metabolism levels in 4 and 6 weeks after surgery, shoulder joint function (Neer score), VAS score before surgery and after 3 and 6 months of surgery, and excellent or good rate of shoulder joint activity after 6 months of surgery were compared between the two groups. Results. The postoperative fracture healing time and start time of shoulder joint training were shorter, and the humeral varus angle and femur height loss were smaller in the abduction frame group than in the sling group (

P
<
0.05

). There was no significant difference in the total incidence of complications between the two groups (3.39% and 13.56%, respectively) (

P
>
0.05

). After 4 or 6 weeks of surgery, the levels of serum osteoprotegerin (OPG) and carboxyterminal propeptide of type I procollagen (PICP) were increased but the levels of tartrate-resistant acid phosphatase-5B (TRAP-5B) were decreased in the two groups with more significant differences in the abduction frame group (

P
<
0.05

). After 6 months of follow-up, 2 cases were lost to follow-up in the abduction frame group and 3 cases in the sling group. Neer scores were increased, while VAS scores were decreased in the two groups in the third or sixth months after surgery with significant differences in the abduction frame group (

P
<
0.05

). The excellent or good rate of shoulder joint activity was 94.74% (54/57) in the abduction frame group, significantly higher than that in the sling group (80.36%; 45/56) (

P
<
0.05

). Conclusion. The fixation effect of new shoulder joint abduction frame is significant after arthroscopic surgery, and patients can carry out functional training as early as possible, which is helpful to promote fracture healing, relieve pain, and restore shoulder joint function with high safety.
A Modified Reptile Search Algorithm for Numerical Optimization Problems
Authors: Yuan Qihang , Zhang Yongde , Dai Xuesong , Zhang Shu
Published: October 2022

The reptile search algorithm (RSA) is a swarm-based metaheuristic algorithm inspired by the encirclement and hunt mechanisms of crocodiles. Compared with other algorithms, RSA is competitive but still suffers from low population diversity, unbalanced exploitation and exploration, and the tendency to fall into local optima. To overcome these shortcomings, a modified variant of RSA, named MRSA, is proposed in this paper. First, an adaptive chaotic reverse learning strategy is employed to enhance the population diversity. Second, an elite alternative pooling strategy is proposed to balance exploitation and exploration. Finally, a shifted distribution estimation strategy is used to correct the evolutionary direction and improve the algorithm performance. Subsequently, the superiority of MRSA is verified using 23 benchmark functions, IEEE CEC2017 benchmark functions, and robot path planning problems. The Friedman test, the Wilcoxon signed-rank test, and simulation results show that the proposed MRSA outperforms other comparative algorithms in terms of convergence accuracy, convergence speed, and stability.
SMAD4 Loss Induces c-MYC–Mediated NLE1 Upregulation to Support Protein Biosynthesis, Colorectal Cancer Growth, and Metastasis
Authors: Loevenich Leon P. , Tschurtschenthaler Markus , Rokavec Matjaz , Silva Miguel G. , Jesinghaus Moritz , Kirchner Thomas , Klauschen Frederick , Saur Dieter , Neumann Jens , Hermeking Heiko , Jung Peter
Published: October 2022

Growth and metastasis of colorectal cancer is closely connected to the biosynthetic capacity of tumor cells, and colorectal cancer stem cells that reside at the top of the intratumoral hierarchy are especially dependent on this feature. By performing disease modeling on patient-derived tumor organoids, we found that elevated expression of the ribosome biogenesis factor NLE1 occurs upon SMAD4 loss in TGFβ1-exposed colorectal cancer organoids. TGFβ signaling-mediated downregulation of NLE1 was prevented by ectopic expression of c-MYC, which occupied an E-box–containing region within the NLE1 promoter. Elevated levels of NLE1 were found in colorectal cancer cohorts compared with normal tissues and in colorectal cancer subtypes characterized by Wnt/MYC and intestinal stem cell gene expression. In colorectal cancer cells and organoids, NLE1 was limiting for de novo protein biosynthesis. Upon NLE1 ablation, colorectal cancer cell lines activated p38/MAPK signaling, accumulated p62- and LC3-positive structures indicative of impaired autophagy, and displayed more reactive oxygen species. Phenotypically, knockout of NLE1 inhibit.ed proliferation, migration and invasion, clonogenicity, and anchorage-independent growth. NLE1 loss also increased the fraction of apoptotic tumor cells, and deletion of TP53 further sensitized NLE1-deficient colorectal cancer cells to apoptosis. In an endoscopy-guided orthotopic mouse transplantation model, ablation of NLE1 impaired tumor growth in the colon and reduced primary tumor-derived liver metastasis. In patients with colorectal cancer, NLE1 mRNA levels predicted overall and relapse-free survival. Taken together, these data reveal a critical role of NLE1 in colorectal cancer growth and progression and suggest that NLE1 represents a potential therapeutic target in colorectal cancer patients.

Significance:

NLE1 limits de novo protein biosynthesis and the tumorigenic potential of advanced colorectal cancer cells, suggesting NLE1 could be targeted to improve the treatment of metastatic colorectal cancer.
Clinical Predictors for Early Mortality of Patients with Acute Basilar Artery Occlusion
Authors: Huang Jiacheng , Wang Maolin , Li Fengli , Kong Weilin , Liu Da , Li Huagang , Zhou Peiyang , Yan Zhizhong , Wang Yan , Song Jiaxing , Luo Weidong , Liu Shuai , Li Linyu , Hu Jinrong , Tian Yan , Qiu Zhongming , Xu Rui , Schonewille Wouter J. , Zi Wenjie , Yang Qingwu
Published: October 2022

Background: Acute ischemic stroke due to basilar artery occlusion (BAO) is associated with the highest mortality in patients with large vessel occlusion. This study aimed to identify modifiable risk factors of early mortality in patients with BAO. Methods and Results: This was a cohort study of consecutive patients with BAO admitted to 47 stroke centers in China between January 2014 and May 2019. The primary outcome was all-cause mortality within 7 days after hospitalization. Of 829 patients, 164 died (0–3 days: 115; 4–7 days: 49) within 7 days after hospitalization. Among pre- and periprocedural variables, higher admission National Institutes of Health Stroke Scale (NIHSS, adjusted OR, 1.06, 95% CI: 1.04–1.09; p < 0.001), lower admission posterior circulation-Alberta Stroke Program Early Computed Tomography Score (pc-ASPECTS, adjusted OR, 0.88, 95% CI: 0.79–0.98; p = 0.02), lower Basilar Artery on Computed Tomography Angiography score (BATMAN, adjusted OR, 0.84, 95% CI: 0.76–0.93; p = 0.001), and recanalization failure (adjusted OR, 2.99, 95% CI: 2.04–4.38; p < 0.001) were independently associated with a higher risk of early mortality. Herniation (adjusted OR, 2.84, 95% CI: 1.52–5.30; p = 0.001) is an independent postprocedural predictor of early mortality. In patients dying ≤3 days, higher NIHSS (p < 0.001), lower pc-ASPECTS (p = 0.01), lower BATMAN (p = 0.004), recanalization failure (p < 0.001), herniation (p = 0.001), gastrointestinal hemorrhage (p = 0.046), and absence of pneumonia (p < 0.001) were independent predictors of early mortality. Higher NIHSS (p = 0.01), recanalization failure (p < 0.001), and pneumonia (p = 0.03) were independently associated with early mortality between 4 and 7 days. Conclusions: Recanalization failure, herniation, gastrointestinal hemorrhage, and pneumonia are potentially modifiable risk factors for early mortality in basilar artery occlusion.
Obesity Exacerbates Lupus Activity in Fc Gamma Receptor IIb Deficient Lupus Mice Partly through Saturated Fatty Acid-Induced Gut Barrier Defect and Systemic Inflammation
Authors: Udompornpitak Kanyarat , Charoensappakit Awirut , Sae-Khow Kritsanawan , Bhunyakarnjanarat Thansita , Dang Cong Phi , Saisorn Wilasinee , Visitchanakun Peerapat , Phuengmaung Pornpimol , Palaga Tanapat , Ritprajak Patcharee , Tungsanga Somkanya , Leelahavanichkul Asada
Published: October 2022

The prevalence of obesity is increasing, and the coexistence of obesity and systemic lupus erythematosus (lupus) is possible. A high-fat diet (HFD) was orally administered for 6 months in female 8-week-old Fc gamma receptor IIb deficient (FcgRIIb−/−) lupus or age and gender-matched wild-type (WT) mice. Lupus nephritis (anti-dsDNA, proteinuria, and increased creatinine), gut barrier defect (fluorescein isothiocyanate dextran), serum lipopolysaccharide (LPS), serum interleukin (IL)-6, liver injury (alanine transaminase), organ fibrosis (liver and kidney pathology), spleen apoptosis (activated caspase 3), and aorta thickness (but not weight gain and lipid profiles) were more prominent in HFD-administered FcgRIIb−/− mice than the obese WT, without injury in regular diet-administered mice (both FcgRIIb−/− and WT). In parallel, combined palmitic acid (PA; a saturated fatty acid) with LPS (PA + LPS) induced higher tumor necrotic factor-α, IL-6, and IL-10 in the supernatant, inflammatory genes (inducible nitric oxide synthase and IL-1β), reactive oxygen species (dihydroethidium), and glycolysis with reduced mitochondrial activity (extracellular flux analysis) when compared with the activation by each molecule alone in both FcgRIIb−/− and WT macrophages. However, the alterations of these parameters were more prominent in PA + LPS-administered FcgRIIb−/− than in the WT cells. In conclusion, obesity accelerated inflammation in FcgRIIb−/− mice, partly due to the more potent responses from the loss of inhibitory FcgRIIb against PA + LPS with obesity-induced gut barrier defect.
A Novel Mutation in Melanocortin Receptor 2 and a Reported Mutation in Melanocortin Receptor 2 Accessory Protein: Three Chinese Cases with Familial Glucocorticoid Deficiency
Authors: Duan Ying , Xia Yu , Gong Zhuwen , Liu Huili , Liang Lili , Zhang Kaichuang , Yang Yi , Wang Ruifang , Xiao Bing , Qiu Wenjuan
Published: October 2022

Background: Familial glucocorticoid deficiency (FGD) is a rare autosomal recessive disease characterized by glucocorticoid deficiency without mineralocorticoid deficiency. We report 3 Chinese patients with MRAP or MC2R mutations. Case Reports: Patient 1 presented with hyperpigmentation. Endocrine investigations revealed low serum cortisol levels and elevated adrenocorticotropic hormone (ACTH) levels. Furthermore, low serum sodium was evident. She was diagnosed with FGD type 2 due to a homozygous mutation in MRAP (c.106+1delG), revealed through exome sequencing (ES). After 2-year treatment with hydrocortisone, skin hyperpigmentation was improved. Patient 2 initially presented with hyponatremia. Low cortisol levels and high levels of ACTH were subsequently detected; he was subjected to a hydrocortisone treatment during which he experienced repeated hypoglycemic attacks and pigmentation. ES revealed the same mutation as in patient 1 in MRAP (c.106+1delG), thus he was diagnosed with FGD type 2. After 6 years of age, his symptoms remarkably improved, and there was no episode of hypoglycemia. Patient 3 mainly presented with hyperpigmentation, hypoglycemic attack, and tall stature. Laboratory findings were normal except for low serum cortisol levels and high ACTH levels. She was diagnosed with FGD type 1 as ES revealed a novel homozygous mutation in MC2R (c.712C>A, p.His238Tyr). After nearly 2 years of hydrocortisone replacement therapy, the excessive growth was reduced to near normal, and the skin color returned to normal. Conclusions: Three patients were diagnosed with FGD (one with FGD type 1 and two with FGD type 2). They all presented with hyperpigmentation and hypoglycemia; however, compared with patient 1, the clinical manifestations of patient 2 were more complicated. Patient 3 had later onset and taller stature than patients 1 and 2. A novel mutation in patient 3 expands the mutation spectrum of MC2R.
Rehabilitación pulmonar: Inclusión en el protocolo de decanulación en pacientes traqueostomizados
Authors: Ceballos-Zuñiga Cecilio Omar
Published: October 2022

Background: Intensive care unit (ICU) survivors from severe COVID-19 acute respiratory distress syndrome (CARDS) with chronic critical illness (CCI) may be considered vast resource consumers with a poor prognosis. We hypothesized that a holistic approach combining an early intensive rehabilitation with a protocol of difficult weaning would improve patient outcomes. Methods: A single-center retrospective study in a five-bed post-ICU weaning and intensive rehabilitation center with a dedicated fitness room specifically equipped to safely deliver physical activity sessions in frail patients with CCI. Results: Among 502 CARDS patients admitted to the ICU from March 2020 to March 2022, 50 consecutive tracheostomized patients were included in the program. After a median of 39 ICU days, 25 days of rehabilitation were needed to restore patients’ autonomy (ADL, from 0 to 6; p < 0.001), to significantly improve their aerobic capacity (6-min walking test distance, from 0 to 253 m; p < 0.001) and to reduce patients’ vulnerability (frailty score, from 7 to 3; p < 0.001) and hospital anxiety and depression scale (HADS, from 18 to 10; p < 0.001). Forty-eight decannulated patients (96%) were discharged home. Conclusion: A protocolized weaning strategy combined with early intensive rehabilitation in a dedicated specialized center boosted the physical and mental recovery.