bluuebunny/crossref_metadata_2025 huggingface dataset:
Transvaginal sonographic cervical length in first and second trimesters in a low‐risk population: a prospective study
Authors: Wulff C. B. , Rode L. , Rosthøj S. , Hoseth E. , Petersen O. B. , Tabor A.
Published: June 2017

Objectives

To assess cervical length (CL) longitudinally between the first and second trimesters and to determine the proportion of women with short CL. The study also aimed to assess if women with short CL at 19–24 weeks' gestation could be identified at the time of combined first‐trimester screening (cFTS) at 11–14 weeks' gestation, in order to determine the potential value of implementation of CL screening for prediction of preterm delivery in a Danish population.

Methods

This was a prospective longitudinal study of women with singleton pregnancy attending three University Hospitals in Denmark for combined first‐trimester screening from 1 November 2013 to 1 December 2014. Exclusion criteria were multiple pregnancy, uterine anomaly, cerclage or progesterone treatment at inclusion. CL was measured on transvaginal sonography at 11–14 weeks (Cx1), 19–21 weeks (Cx2) and 23–24 weeks (Cx3), by trained operators as a straight line from external to internal os. Women with CL ≤ 25 mm were referred to a maternal–fetal medicine specialist for treatment according to a standardized management protocol.

Results

Of the 4904 eligible women, 3477 (71%) participated and had Cx1 recorded. Of those, 3232 (93.0%) had CL measured on all three scans. Median Cx1 was 37 mm, and median Cx2 and Cx3 were 40 mm. The proportion of women with CL ≤ 25 mm increased with gestational age, from 0.41% (95% CI, 0.19–0.62%) at Cx1 to 1.79% (95% CI, 1.34–2.24%) at Cx3. In total, the proportion of women with second‐trimester CL (Cx2 or Cx3) ≤ 25 mm was 2.0% (n = 67), of which 38.8% (n = 26) were detected at 19–21 weeks. The probability of short CL between 19 and 24 weeks was greater for those with shorter first‐trimester CL. It was nearly nine‐fold higher for women with Cx1 ≤ 25 mm compared with Cx1 ≥ 35 mm (17% vs 2%). The performance of Cx1 for prediction of short second‐trimester CL was 50% at a 10% false‐positive rate. It was found that more than 1500 women would need to be screened for short CL at 19–21 weeks to prevent one case of spontaneous preterm delivery before 34 weeks in a population such as the one in this study.

Conclusions

There is an association between first‐trimester CL and risk of short cervix in the second trimester. Once short CL was observed, risk of preterm delivery was greatly increased. However, whether universal CL screening should be implemented in this low‐risk population depends on cost–benefit analysis taking into account the low proportions of women with short CL and at risk for preterm delivery. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.
Deep neuromuscular blockade improves surgical conditions during low-pressure pneumoperitoneum laparoscopic donor nephrectomy
Authors: Özdemir-van Brunschot D. M. D. , Braat A. E. , van der Jagt M. F. P. , Scheffer G. J. , Martini C. H. , Langenhuijsen J. F. , Dam R. E. , Huurman V. A. , Lam D. , d’Ancona F. C. , Dahan A. , Warlé M. C.
Published: June 2017

Background

Evidence indicates that low-pressure pneumoperitoneum (PNP) reduces postoperative pain and analgesic consumption. A lower insufflation pressure may hamper visibility and working space. The aim of the study is to investigate whether deep neuromuscular blockade (NMB) improves surgical conditions during low-pressure PNP.

Methods

This study was a blinded randomized controlled multicenter trial. 34 kidney donors scheduled for laparoscopic donor nephrectomy randomly received low-pressure PNP (6 mmHg) with either deep (PTC 1–5) or moderate NMB (TOF 0–1). In case of insufficient surgical conditions, the insufflation pressure was increased stepwise. Surgical conditions were rated by the Leiden-Surgical Rating Scale (L-SRS) ranging from 1 (extremely poor) to 5 (optimal).

Results

Mean surgical conditions were significantly better for patients allocated to a deep NMB (SRS 4.5 versus 4.0; p < 0.01). The final insufflation pressure was 7.7 mmHg in patients with deep NMB as compared to 9.1 mmHg with moderate NMB (p = 0.19). The cumulative opiate consumption during the first 48 h was significantly lower in patients receiving deep NMB, while postoperative pain scores were similar. In four patients allocated to a moderate NMB, a significant intraoperative complication occurred, and in two of these patients a conversion to an open procedure was required.

Conclusions

Our data show that deep NMB facilitates the use of low-pressure PNP during laparoscopic donor nephrectomy by improving the quality of the surgical field. The relatively high incidence of intraoperative complications indicates that the use of low pressure with moderate NMB may compromise safety during LDN. Clinicaltrials.gov identifier: NCT 02602964.
Cysteamine revisited: repair of arginine to cysteine mutations
Authors: Gallego‐Villar L. , Hannibal Luciana , Häberle J. , Thöny B. , Ben‐Omran T. , Nasrallah G. K. , Dewik Al‐N. , Kruger W. D. , Blom H. J.
Published: June 2017

Cysteamine is a small aminothiol endogenously derived from coenzyme A degradation. For some decades, synthetic cysteamine has been employed for the treatment of cystinosis, and new uses of the drug continue to emerge. In this review, we discuss the role of cysteamine in cellular and extracellular homeostasis and focus on the potential use of aminothiols to reconstitute the function of proteins harboring arginine (Arg) to cysteine (Cys) mutations, via repair of the Cys residue into a moiety that introduces an amino group, as seen in basic amino acid residues Lys and Arg. Cysteamine has been utilized in vitro and ex vivo in four different genetic disorders, and thus provides “proof of principle” that aminothiols can modify Cys residues. Other aminothiols such as mercaptoethylguanidine (MEG) with closer structural resemblance to the guanidinium moiety of Arg are under examination for their predicted enhanced capacity to reconstitute loss of function. Although the use of aminothiols holds clinical potential, more studies are required to refine specificity and treatment design. The efficacy of aminothiols to target proteins may vary substantially depending on their specific extracellular and intracellular locations. Redox potential, pH, and specific aminothiol abundance in each physiological compartment are expected to influence the reactivity and turnover of cysteamine and analogous drugs. Upcoming research will require the use of suitable cell and animal models featuring Arg to Cys mutations. Since, in general, Arg to Cys changes comprise about 8% of missense mutations, repair of this specific mutation may provide promising avenues for many genetic diseases.
Elaboration of the Reciprocal‐Engagement Model of Genetic Counseling Practice: a Qualitative Investigation of Goals and Strategies
Authors: Redlinger‐Grosse Krista , Veach Patricia McCarthy , LeRoy Bonnie S. , Zierhut Heather
Published: June 2017

As the genetic counseling field evolves, a comprehensive model of practice is critical. The Reciprocal‐Engagement Model (REM) consists of 5 tenets and 17 goals. Lacking in the REM, however, are well‐articulated counselor strategies and behaviors. The purpose of the present study was to further elaborate and provide supporting evidence for the REM by identifying and mapping genetic counseling strategies to the REM goals. A secondary, qualitative analysis was conducted on data from two prior studies: 1) focus group results of genetic counseling outcomes (Redlinger‐Grosse et al., Journal of Genetic Counseling, 2015); and 2) genetic counselors’ examples of successful and unsuccessful genetic counseling sessions (Geiser et al. 2009). Using directed content analysis, 337 unique strategies were extracted from focus group data. A Q‐sort of the 337 strategies yielded 15 broader strategy domains that were then mapped to the successful and unsuccessful session examples. Differing prevalence of strategy domains identified in successful sessions versus the prevalence of domains identified as lacking in unsuccessful sessions provide further support for the REM goals. The most prevalent domains for successful sessions were Information Giving and Use Psychosocial Skills and Strategies; and for unsuccessful sessions, Information Giving and Establish Working Alliance. Identified strategies support the REM's reciprocal nature, especially with regard to addressing patients’ informational and psychosocial needs. Patients’ contributions to success (or lack thereof) of sessions was also noted, supporting a REM tenet that individual characteristics and the counselor‐patient relationship are central to processes and outcomes. The elaborated REM could be used as a framework for certain graduate curricular objectives, and REM components could also inform process and outcomes research studies to document and further characterize genetic counselor strategies.
Unsteady effects of strong shock-wave/boundary-layer interaction at high Reynolds number
Authors: Pasquariello Vito , Hickel Stefan , Adams Nikolaus A.
Published: June 2017

We analyse the low-frequency dynamics of a high Reynolds number impinging shock-wave/turbulent boundary-layer interaction (SWBLI) with strong mean-flow separation. The flow configuration for our grid-converged large-eddy simulations (LES) reproduces recent experiments for the interaction of a Mach 3 turbulent boundary layer with an impinging shock that nominally deflects the incoming flow by$19.6^{\circ }$. The Reynolds number based on the incoming boundary-layer thickness of$Re_{\unicode[STIX]{x1D6FF}_{0}}\approx 203\times 10^{3}$is considerably higher than in previous LES studies. The very long integration time of$3805\unicode[STIX]{x1D6FF}_{0}/U_{0}$allows for an accurate analysis of low-frequency unsteady effects. Experimental wall-pressure measurements are in good agreement with the LES data. Both datasets exhibit the distinct plateau within the separated-flow region of a strong SWBLI. The filtered three-dimensional flow field shows clear evidence of counter-rotating streamwise vortices originating in the proximity of the bubble apex. Contrary to previous numerical results on compression ramp configurations, these Görtler-like vortices are not fixed at a specific spanwise position, but rather undergo a slow motion coupled to the separation-bubble dynamics. Consistent with experimental data, power spectral densities (PSD) of wall-pressure probes exhibit a broadband and very energetic low-frequency component associated with the separation-shock unsteadiness. Sparsity-promoting dynamic mode decompositions (SPDMD) for both spanwise-averaged data and wall-plane snapshots yield a classical and well-known low-frequency breathing mode of the separation bubble, as well as a medium-frequency shedding mode responsible for reflected and reattachment shock corrugation. SPDMD of the two-dimensional skin-friction coefficient further identifies streamwise streaks at low frequencies that cause large-scale flapping of the reattachment line. The PSD and SPDMD results of our impinging SWBLI support the theory that an intrinsic mechanism of the interaction zone is responsible for the low-frequency unsteadiness, in which Görtler-like vortices might be seen as a continuous (coherent) forcing for strong SWBLI.
Feedback loop and upwind-propagating waves in ideally expanded supersonic impinging round jets
Authors: Bogey Christophe , Gojon Romain
Published: June 2017

The aeroacoustic feedback loop establishing in a supersonic round jet impinging on a flat plate normally has been investigated by combining compressible large-eddy simulations and modelling of that loop. At the exit of a straight pipe nozzle of radius $r_{0}$, the jet is ideally expanded, and has a Mach number of 1.5 and a Reynolds number of$6\times 10^{4}$. Four distances between the nozzle exit and the flat plate, equal to$6r_{0}$,$8r_{0}$,$10r_{0}$and$12r_{0}$, have been considered. In this way, the variations of the convection velocity of the shear-layer turbulent structures according to the nozzle-to-plate distance are shown. In the spectra obtained inside and outside of the flow near the nozzle, several tones emerge at Strouhal numbers in agreement with measurements in the literature. At these frequencies, by applying Fourier decomposition to the pressure fields, hydrodynamic-acoustic standing waves containing a whole number of cells between the nozzle and the plate and axisymmetric or helical jet oscillations are found. The tone frequencies and the mode numbers inferred from the standing-wave patterns are in line with the classical feedback-loop model, in which the loop is closed by acoustic waves outside the jet. The axisymmetric or helical nature of the jet oscillations at the tone frequencies is also consistent with a wave analysis using a jet vortex-sheet model, providing the allowable frequency ranges for the upstream-propagating acoustic wave modes of the jet. In particular, the tones are located on the part of the dispersion relations of the modes where these waves have phase and group velocities close to the ambient speed of sound. Based on the observation of the pressure fields and on frequency–wavenumber spectra on the jet axis and in the shear layers, such waves are identified inside the present jets, for the first time to the best of our knowledge, for a supersonic jet flow. This study thus suggests that the feedback loop in ideally expanded impinging jets is completed by these waves.
Velocity fluctuations generated by the flow through a random array of spheres: a model of bubble-induced agitation
Authors: Amoura Zouhir , Besnaci Cédric , Risso Frédéric , Roig Véronique
Published: June 2017

This work reports an experimental investigation of the flow through a random array of fixed solid spheres. The volume fraction of the spheres is 2 %, and the Reynolds number $Re$ based on the sphere diameter and the average flow velocity is varied from 120 to 1040. Using time and spatial averaging, the fluctuations have been decomposed into two contributions of different natures: a spatial fluctuation that accounts for the strong inhomogeneity of the flow around each sphere, and a time fluctuation that comes from the instability of the flow at large enough Reynolds numbers. The evolutions of these two contributions with the Reynolds number are different, so that their relative importance varies. However, when each is normalized by using its own variance and the integral length scales of the fluctuations, their spectra and probability density functions (PDFs) are almost independent of $Re$. The spatial fluctuation mostly comes from the velocity deficit in the wakes of the spheres, and is thus dominated by scales larger than one or two sphere diameters. It is found to be responsible for the asymmetry of the PDFs of the vertical fluctuations and of the major part of the anisotropy level between the vertical and the horizontal components of the fluctuations. The time fluctuation dominates at scales smaller than the integral length scale. It is isotropic and its PDFs, well described by an exponential distribution, are non-Gaussian. The spectra of the spatial and the time fluctuations both show an evolution as the power $-3$ of the wavenumber, but not exactly in the same subrange. All these properties are found in remarkable agreement with the results of both experimental investigations and large eddy simulations (LES) of a homogeneous bubble swarm. This confirms that the main mechanism responsible for the production of bubble-induced fluctuations is the interaction of the velocity disturbances caused by obstacles immersed in a flow and that the structure of this agitation is weakly dependent on the precise nature of the obstacles. The understanding and the modelling of the agitation generated by the motion of a dispersed phase, such as the bubble-induced agitation, therefore require one to distinguish between the roles of these two contributions.
Subcritical Sevastyanov branching processes with nonhomogeneous Poisson immigration
Authors: Hyrien Ollivier , Mitov Kosto V. , Yanev Nikolay M.
Published: June 2017

We consider a class of Sevastyanov branching processes with nonhomogeneous Poisson immigration. These processes relax the assumption required by the Bellman–Harris process which imposes the lifespan and offspring of each individual to be independent. They find applications in studies of the dynamics of cell populations. In this paper we focus on the subcritical case and examine asymptotic properties of the process. We establish limit theorems, which generalize classical results due to Sevastyanov and others. Our key findings include a novel law of large numbers and a central limit theorem which emerge from the nonhomogeneity of the immigration process.
On the measure of Voronoi cells
Authors: Devroye Luc , Györfi László , Lugosi Gábor , Walk Harro
Published: June 2017

We study the measure of a typical cell in a Voronoi tessellation defined by n independent random points X1, . . ., Xn drawn from an absolutely continuous probability measure μ with density f in ℝd. We prove that the asymptotic distribution of the measure – with respect to dμ = f(x)dx – of the cell containing X1 given X1 = x is independent of x and the density f. We determine all moments of the asymptotic distribution and show that the distribution becomes more concentrated as d becomes large. In particular, we show that the variance converges to 0 exponentially fast in d. We also obtain a bound independent of the density for the rate of convergence of the diameter of a typical Voronoi cell.
Temporal dynamics of encrusting communities during the Late Devonian: a case study from the Central Devonian Field, Russia
Authors: Zatoń Michał , Borszcz Tomasz , Rakociński Michał
Published: June 2017

In this study we focused on the dynamics of encrusting assemblages preserved on brachiopod hosts collected from upper Frasnian and lower Famennian deposits of the Central Devonian Field, Russia. Because the encrusted brachiopods come from deposits bracketing the Frasnian/Famennian (F/F) boundary, the results also shed some light on ecological differences in encrusting communities before and after the Frasnian–Famennian (F-F) event. To explore the diversity dynamics of encrusting assemblages, we analyzed more than 1300 brachiopod valves (substrates) from two localities. Taxon accumulation plots and shareholder quorum subsampling (SQS) routines indicated that a reasonably small sample of brachiopod host valves (n=50) is sufficient to capture the majority of the encrusting genera recorded at a given site. The richness of encrusters per substrate declined simultaneously with the number of encrusting taxa in the lower Famennian, accompanied by a decrease in epibiont abundance, with a comparable decrease in mean encrustation intensity (percentage of bioclasts encrusted by one or more epibionts). Epibiont abundance and occupancy roughly mirror each other. Strikingly, few ecological characteristics are correlated with substrate size, possibly reflecting random settlement of larvae. Evenness, which is negatively correlated with substrate size, shows greater within-stage variability among samples than between Frasnian and Famennian intervals and may indicate the instability of early Famennian biocenoses following the faunal turnover. The occurrence distribution of encrusters points to nonrandom associations and exclusions among several encrusting taxa. However, abundance and occupancy of microconchids remained relatively stable throughout the sampled time interval. The notable decline in abundance (~60%) and relatively minor decline in diversity (~30%) suggest jointly that encrusting communities experienced ecological collapse rather than a major mass extinction event. The differences between the upper Frasnian and lower Famennian encrusting assemblages may thus record a turnover associated with the F-F event.
Citrulline decreases hepatic endotoxin-induced injury in fructose-induced non-alcoholic liver disease: anex vivostudy in the isolated perfused rat liver
Authors: Ouelaa Wassila , Jegatheesan Prasanthi , M’bouyou-Boungou Japhète , Vicente Christelle , Nakib Samir , Nubret Esther , De Bandt Jean-Pascal
Published: June 2017

Steatosis can sensitise the liver to various challenges and favour the development of non-alcoholic fatty liver disease (NAFLD). In this context, fructose feeding promotes endotoxin translocation from the gut, contributing to disease progression via an inflammatory process. Citrulline is protective against fructose-induced NAFLD; we hypothesised that this property might be related to its anti-inflammatory and antioxidative action against endotoxin-induced hepatic injuries. This hypothesis was evaluated in a model of perfused liver isolated from NAFLD rats. Male Sprague–Dawley rats (n30) were fed either a standard rodent chow or a 60 % fructose diet alone, or supplemented with citrulline (1 g/kg per d) for 4 weeks. After an evaluation of their metabolic status, fasted rats received an intraperitoneal injection of lipopolysaccharide (LPS) (2·5 mg/kg). After 1 h, the livers were isolated and perfused for 1 h to study liver function and metabolism, inflammation and oxidative status.In vivo, citrulline significantly decreased dyslipidaemia induced by a high-fructose diet and insulin resistance. In the isolated perfused rat livers, endotoxaemia resulted in higher cytolysis (alanine aminotransferase release) and higher inflammation (Toll-like receptor 4) in livers of fructose-fed rats, and it was prevented by citrulline supplementation. Oxidative stress and antioxidative defences were similar in all three groups. Amino acid exchanges and metabolism (ammonia and urea release) were only slightly different between the three groups. In this context of mild steatosis, our results suggest that fructose-induced NAFLD leads to an increased hepatic sensitivity to LPS-induced inflammation. Citrulline-induced restriction of the inflammatory process may thus contribute to the prevention of NAFLD.
Evaluation of urinary resveratrol as a biomarker of dietary resveratrol intake in the European Prospective Investigation into Cancer and Nutrition (EPIC) study
Authors: Zamora-Ros Raul , Rothwell Joseph A. , Achaintre David , Ferrari Pietro , Boutron-Ruault Marie-Christine , Mancini Francesca R. , Affret Aurelie , Kühn Tilman , Katzke Verena , Boeing Heiner , Küppel Sven , Trichopoulou Antonia , Lagiou Pagona , La Vecchia Carlo , Palli Domenico , Contiero Paolo , Panico Salvatore , Tumino Rosario , Ricceri Fulvio , Noh Hwayoung , Freisling Heinz , Romieu Isabelle , Scalbert Augustin
Published: June 2017

In vitro studies have shown several beneficial properties of resveratrol. Epidemiological evidence is still scarce, probably because of the difficulty in estimating resveratrol exposure accurately. The current study aimed to assess the relationships between acute and habitual dietary resveratrol and wine intake and urinary resveratrol excretion in a European population. A stratified random subsample of 475 men and women from four countries participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) cross-sectional study, who had provided 24-h urine samples and completed a 24-h dietary recall (24-HDR) on the same day, were included. Acute and habitual dietary data were collected using standardised 24-HDR software and a validated country-specific dietary questionnaire, respectively. Phenol-Explorer was used to estimate the intake of resveratrol and other stilbenes. Urinary resveratrol was analysed using tandem MS. Spearman’s correlation coefficients between estimated dietary intakes of resveratrol and other stilbenes and consumption of wine, their main food source, were very high (r>0·9) when measured using dietary questionnaires and were slightly lower with 24-HDR (r>0·8). Partial Spearman’s correlations between urinary resveratrol excretion and intake of resveratrol, total stilbenes or wine were found to be higher when using the 24-HDR (R2partial approximately 0·6) than when using the dietary questionnaires (R2partial approximately 0·5). Moderate to high correlations between dietary resveratrol, total stilbenes and wine, and urinary resveratrol concentrations were observed. These support the earlier findings that 24-h urinary resveratrol is an effective biomarker of both resveratrol and wine intakes. These correlations also support the validity of the estimation of resveratrol intake using the dietary questionnaire and Phenol-Explorer.
Depression and pain: primary data and meta-analysis among 237 952 people across 47 low- and middle-income countries
Authors: Stubbs B. , Vancampfort D. , Veronese N. , Thompson T. , Fornaro M. , Schofield P. , Solmi M. , Mugisha J. , Carvalho A. F. , Koyanagi A.
Published: June 2017

Background

Depression and pain are leading causes of global disability. However, there is a paucity of multinational population data assessing the association between depression and pain, particularly among low- and middle-income countries (LMICs) where both are common. Therefore, we investigated this association across 47 LMICs.

Methods

Community-based data on 273 952 individuals from 47 LMICs were analysed. Multivariable logistic and linear regression analyses were performed to assess the association between the International Classification of Diseases, 10th Revision depression/depression subtypes (over the past 12 months) and pain in the previous 30 days based on self-reported data. Country-wide meta-analysis adjusting for age and sex was also conducted.

Results

The prevalence of severe pain was 8.0, 28.2, 20.2, and 34.0% for no depression, subsyndromal depression, brief depressive episode, and depressive episode, respectively. Logistic regression adjusted for socio-demographic variables, anxiety and chronic medical conditions (arthritis, diabetes, angina, asthma) demonstrated that compared with no depression, subsyndromal depression, brief depressive episode, and depressive episode were associated with a 2.16 [95% confidence interval (CI) 1.83–2.55], 1.45 (95% CI 1.22–1.73), and 2.11 (95% CI 1.87–2.39) increase in odds of severe pain, respectively. Similar results were obtained when a continuous pain scale was used as the outcome. Depression was significantly associated with severe pain in 44/47 countries with a pooled odds ratio of 3.93 (95% CI 3.54–4.37).

Conclusion

Depression and severe pain are highly comorbid across LMICs, independent of anxiety and chronic medical conditions. Whether depression treatment or pain management in patients with comorbid pain and depression leads to better clinical outcome is an area for future research.
Ageing in place: residential satisfaction in Polish housing-estate communities
Authors: GORCZYCA KATARZYNA , GRABIŃSKI TADEUSZ
Published: June 2017

During the period of economic and political transformations in Poland, considerable changes occurred in large housing estates, while demographic transformation and housing-stock ageing starting to aggravate social and spatial problems. The serious issue currently dominating such estates concerns the process of natural ageing of the housing stock and residential ageing which is associated with the reduction of residential mobility and demographic ageing of people living in the large housing estates. This paper intends to identify ageing of the estates’ population in the context of the evaluation of residential life quality. The authors concentrated on analysis of 2002 and 2011 statistical data, as well as data collected during their field surveys conducted in 2010–2012. Detailed studies were carried out on seven estates in five cities and towns in Poland (Kraków, Poznań, Tarnów, Żyrardów and Dzierżoniów). To identify the residential standard and quality of life, surveys were conducted on housing estates which allowed the researchers to establish the essential factors which determined the level of satisfaction of older people with regard to their place of residence. The authors also determined the course of changes in the perception of particular aspects of life quality in the process of residents’ ageing in the selected places of residence.
Deformation of an elastic substrate due to a resting sessile droplet
Authors: BARDALL AARON , DANIELS KAREN E. , SHEARER MICHAEL
Published: June 2017

On a sufficiently soft substrate, a resting fluid droplet will cause significant deformation of the substrate. This deformation is driven by a combination of capillary forces at the contact line and the fluid pressure at the solid surface. These forces are balanced at the surface by the solid traction stress induced by the substrate deformation. Young's Law, which predicts the equilibrium contact angle of the droplet, also indicates an a priori radial force balance for rigid substrates, but not necessarily for soft substrates that deform under loading. It remains an open question whether the contact line transmits a non-zero force tangent to the substrate surface in addition to the conventional normal (vertical) force. We present an analytic Fourier transform solution technique that includes general interfacial energy conditions, which govern the contact angle of a 2D droplet. This includes evaluating the effect of gravity on the droplet shape in order to determine the correct fluid pressure at the substrate surface for larger droplets. Importantly, we find that in order to avoid a strain singularity at the contact line under a non-zero tangential contact line force, it is necessary to include a previously neglected horizontal traction boundary condition. To quantify the effects of the contact line and identify key quantities that will be experimentally accessible for testing the model, we evaluate solutions for the substrate surface displacement field as a function of Poisson's ratio and zero/non-zero tangential contact line forces.
A dosimetric comparison of craniospinal irradiation using TomoDirect radiotherapy, TomoHelical radiotherapy and 3D conventional radiotherapy
Authors: Tsang Shirley W. S. , Collins Mark , Wong Jacky T. L. , Chiu George
Published: June 2017

Aim

The purpose of this study was to dosimetrically compare TomoDirect, TomoHelical and linear accelerator-based 3D-conformal radiotherapy (Linac-3DCRT) for craniospinal irradiation (CSI) in the treatment of medulloblastoma.

Methods

Five CSI patients were replanned with Linac-3DCRT, TomoHelical, TomoDirect-3DCRT and TomoDirect-intensity-modulated radiotherapy (IMRT). Dose of 36 Gy in 20 fractions was prescribed to the planning target volume (PTV). Homogeneity index (HI), non-target integral dose (NTID), dose–volume histograms, organs-at-risk (OARs)Dmax,Dmeanand treatment times were compared.

Results

TomoHelical achieved the best PTV homogeneity compared with Linac-3DCRT, TomoDirect-3DCRT and TomoDirect-IMRT (HI of 3·6 versus 20·9, 8·7 and 9·4%, respectively). TomoDirect-IMRT achieved the lowest NTID compared with TomoDirect-3DCRT, TomoHelical and Linac-3DCRT (141 J versus 151 J, 181 J and 250 J), indicating least biological damage to normal tissues. TomoHelical plans achieved the lowestDmaxin all organs except the breasts, and lowestDmeanfor most OARs, except in laterally situated OARs, where TomoDirect triumphed. Beam-on time was longest for TomoHelical, followed by TomoDirect and Linac-3DCRT.

Findings

TomoDirect has the potential to lower NTID and shorten treatment times compared with TomoHelical. It reduces PTV inhomogeneity and better spares OARs compared with Linac-3DCRT. Therefore, TomoDirect may be a CSI treatment alternative to TomoHelical and in place of Linac-3DCRT.
Priorities for African youth for engaging in DOHaD
Authors: Macnab A. J. , Mukisa R.
Published: June 2017

A challenge for implementing DOHaD-defined health promotion is how to engage the at-risk population. The WHO Health Promoting School (HPS) model has proven success engaging youth and improving health behaviors. Hence, we introduced DOHaD concepts to 151 pupils aged 12–15 years in three HPS programs in rural Uganda, inquired what factors would make DOHaD-related health promotion resonate with them, and discussed how they recommended making learning about DOHaD acceptable to youth. Economic factors were judged the most compelling; with nutrition and responsive care elements next in importance. Suggested approaches included: teach how good health is beneficial, what works and why, and give tools to use to achieve it, and make information positive rather than linked to later harm. Involve youth in making DOHaD learning happen, make being a parent sound interesting, and include issues meaningful to boys. These are the first data from youth charged with addressing their engagement in the DOHaD agenda.
Predictors of adults’ body mass index and the association with index child’s infant birth weight, in the Lifeways Cross-Generation Cohort Study of a thousand families in the Republic of Ireland
Authors: McKey S. , Heinen M. , Mehegan J. , Somerville R. , Khalil H. , Segurado R. , Murrin C. , Kelleher C. C. ,   for the Lifeways Cross-Generation Cohort Study Steering Group
Published: June 2017

The Lifeways study is novel in having information on three generations of the same families. It is well established that infant birth weight (IBW) predicts individuals’ risk of adult chronic disease and more recently studies report cross-generation transmission of risk patterns. The aims of this analysis were to examine whether adults’ birth weights were associated with measures of own health status or social position and to relate adults’ birth weights to that of the index child’s IBW. Finally, we assessed whether birth weight of either adults or children was associated with adult body mass index (BMI) of parents and grandparents. We included 1075 children whose IBW was recorded at recruitment from hospital records and 2546 adult cohort members followed from 2001 until 2014. At baseline, a sub-group of 920 adults had reported own birth weight (RBW). Results showed male adults’ RBW were significantly higher than females’ (P=0.001). Mothers’ RBW was significantly correlated with IBW (r=0.178, P<0.001). In mixed effects linear models with BMI as the outcome variable, of all adults, and in sub-groups of adults with RBW and of mothers only, the IBW was associated with adult BMI adjusting for other predictors. Adults’ BMI was positively associated with age (P=0.013), index child’s IBW (P=0.001), gender (P<0.001) but not own RBW, adjusting for family identification number. When mothers were removed from the adult models however, IBW ceased to be associated with BMI, a final model showed RBW being associated with adult BMI (P=0.04). There are cross-generational associations in the Lifeways cohort, the maternal association being stronger.
No additional prognostic value for MRE11 in squamous cell carcinomas of the anus treated with chemo-radiotherapy
Authors: Walker Alexandra K , Kartsonaki Christiana , Collantes Elena , Nicholson Judith , Gilbert Duncan C , Kiltie Anne E
Published: June 2017

Background:

The majority of anal cancers (84–95%) are driven by infection with human papillomavirus (HPV). HPV-positive tumours show significantly better responses to chemo-radiotherapy when compared with HPV-negative tumours. HPV infection is linked to alterations in DNA damage response proteins, including MRE11. MRE11 is a potential predictive biomarker for response to radiotherapy in muscle-invasive bladder cancer and may hold predictive power in other cancers.

Methods:

Using a previously reported cohort, we evaluated the levels of MRE11 in anal cancer and assessed its predictive value in this disease.

Results:

We found no association between the level of MRE11 and relapse-free survival following chemo-radiotherapy.

Conclusions:

MRE11 has no predictive value in the analysis of relapse-free survival after chemo-radiotherapy in anal cancer and does not add to the prognostic value of p16 and tumour-infiltrating lymphocyte scores. Further investigation into the role of DNA repair proteins in anal cancer is required.
Long non-coding RNA ATB promotes malignancy of esophageal squamous cell carcinoma by regulating miR-200b/Kindlin-2 axis
Authors: Li Zhongwen , Wu Xiaoliang , Gu Ling , Shen Qi , Luo Wen , Deng Chuangzhong , Zhou Qianghua , Chen Xinru , Li Yanjie , Lim ZuanFu , Wang Xing , Wang Jiahong , Yang Xianzi
Published: June 2017

Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer-related death, especially in China. In addition, the prognosis of late stage patients is extremely poor. However, the biological significance of the long non-coding RNA lnc-ATB and its potential role in ESCC remain to be documented. In this study, we investigated the role of lnc-ATB and the underlying mechanism promoting its oncogenic activity in ESCC. Expression of lnc-ATB was higher in ESCC tissues and cell lines than that in normal counterparts. Upregulated lnc-ATB served as an independent prognosis predictor of ESCC patients. Moreover, loss-of-function assays in ESCC cells showed that knockdown of lnc-ATB inhibited cell proliferation and migration both in vitro and in vivo. Mechanistic investigation indicated that lnc-ATB exerted oncogenic activities via regulating Kindlin-2, as the anti-migration role of lnc-ATB silence was attenuated by ectopic expression of Kindlin-2. Further analysis showed that lnc-ATB functions as a molecular sponge for miR-200b and Kindlin-2. Dysregulated miR-200b/Kindlin-2 signaling mediated the oncogenic activity of lnc-ATB in ESCC. Our results suggest that lnc-ATB predicts poor prognosis and may serve as a potential therapeutic target for ESCC patients.
Electronic metal-support interaction enhanced oxygen reduction activity and stability of boron carbide supported platinum
Authors: Jackson Colleen , Smith Graham T. , Inwood David W. , Leach Andrew S. , Whalley Penny S. , Callisti Mauro , Polcar Tomas , Russell Andrea E. , Levecque Pieter , Kramer Denis
Published: June 2017

Catalysing the reduction of oxygen in acidic media is a standing challenge. Although activity of platinum, the most active metal, can be substantially improved by alloying, alloy stability remains a concern. Here we report that platinum nanoparticles supported on graphite-rich boron carbide show a 50–100% increase in activity in acidic media and improved cycle stability compared to commercial carbon supported platinum nanoparticles. Transmission electron microscopy and x-ray absorption fine structure analysis confirm similar platinum nanoparticle shapes, sizes, lattice parameters, and cluster packing on both supports, while x-ray photoelectron and absorption spectroscopy demonstrate a change in electronic structure. This shows that purely electronic metal-support interactions can significantly improve oxygen reduction activity without inducing shape, alloying or strain effects and without compromising stability. Optimizing the electronic interaction between the catalyst and support is, therefore, a promising approach for advanced electrocatalysts where optimizing the catalytic nanoparticles themselves is constrained by other concerns.
Differential effects of developmental thermal plasticity across three generations of guppies (Poecilia reticulata): canalization and anticipatory matching
Authors: Le Roy Amélie , Loughland Isabella , Seebacher Frank
Published: June 2017

Developmental plasticity can match offspring phenotypes to environmental conditions experienced by parents. Such epigenetic modifications are advantageous when parental conditions anticipate offspring environments. Here we show firstly, that developmental plasticity manifests differently in males and females. Secondly, that under stable conditions, phenotypic responses (metabolism and locomotion) accumulate across several generations. Metabolic scope in males was greater at warmer test temperatures (26–36 °C) in offspring bred at warm temperatures (29–30 °C) compared to those bred at cooler temperatures (22–23 °C), lending support to the predictive adaptive hypothesis. However, this transgenerational matching was not established until the second (F2) generation. For other responses, e.g. swimming performance in females, phenotypes of offspring bred in different thermal environments were different in the first (F1) generation, but became more similar across three generations, implying canalization. Thirdly, when environments changed across generations, the grandparental environment affected offspring phenotypes. In females, the mode of the swimming thermal performance curve shifted to coincide with the grandparental rather than the parental or offspring developmental environments, and this lag in response may represent a cost of plasticity. These findings show that the effects of developmental plasticity differ between traits, and may be modulated by the different life histories of males and females.
Multilevel Evolutionary Algorithm that Optimizes the Structure of Scale-Free Networks for the Promotion of Cooperation in the Prisoner’s Dilemma game
Authors: Liu Penghui , Liu Jing
Published: June 2017

Understanding the emergence of cooperation has long been a challenge across disciplines. Even if network reciprocity reflected the importance of population structure in promoting cooperation, it remains an open question how population structures can be optimized, thereby enhancing cooperation. In this paper, we attempt to apply the evolutionary algorithm (EA) to solve this highly complex problem. However, as it is hard to evaluate the fitness (cooperation level) of population structures, simply employing the canonical evolutionary algorithm (EA) may fail in optimization. Thus, we propose a new EA variant named mlEA-CPD-SFN to promote the cooperation level of scale-free networks (SFNs) in the Prisoner’s Dilemma Game (PDG). Meanwhile, to verify the preceding conclusions may not be applied to this problem, we also provide the optimization results of the comparative experiment (EAcluster), which optimizes the clustering coefficient of structures. Even if preceding research concluded that highly clustered scale-free networks enhance cooperation, we find EAcluster does not perform desirably, while mlEA-CPD-SFN performs efficiently in different optimization environments. We hope that mlEA-CPD-SFN may help promote the structure of species in nature and that more general properties that enhance cooperation can be learned from the output structures.
Trait-based model development to support breeding programs. A case study for salt tolerance and rice
Authors: Paleari Livia , Movedi Ermes , Confalonieri Roberto
Published: June 2017

Eco-physiological models are increasingly used to analyze G × E × M interactions to support breeding programs via the design of ideotypes for specific contexts. However, available crop models are only partly suitable for this purpose, since they often lack clear relationships between parameters and traits breeders are working on. Taking salt stress tolerance and rice as a case study, we propose a paradigm shift towards the building of ideotyping-specific models explicitly around traits involved in breeding programs. Salt tolerance is a complex trait relying on different physiological processes that can be alternatively selected to improve the overall crop tolerance. We developed a new model explicitly accounting for these traits and we evaluated its performance using data from growth chamber experiments (e.g., R2ranged from 0.74 to 0.94 for the biomass of different plant organs). Using the model, we were able to show how an increase in the overall tolerance can derive from completely different physiological mechanisms according to soil/water salinity dynamics. The study demonstrated that a trait-based approach can increase the usefulness of mathematical models for supporting breeding programs.
A Dynamic Precision Evaluation Method for the Star Sensor in the Stellar-Inertial Navigation System
Authors: Lu Jiazhen , Lei Chaohua , Yang Yanqiang
Published: June 2017

Integrating the advantages of INS (inertial navigation system) and the star sensor, the stellar-inertial navigation system has been used for a wide variety of applications. The star sensor is a high-precision attitude measurement instrument; therefore, determining how to validate its accuracy is critical in guaranteeing its practical precision. The dynamic precision evaluation of the star sensor is more difficult than a static precision evaluation because of dynamic reference values and other impacts. This paper proposes a dynamic precision verification method of star sensor with the aid of inertial navigation device to realize real-time attitude accuracy measurement. Based on the gold-standard reference generated by the star simulator, the altitude and azimuth angle errors of the star sensor are calculated for evaluation criteria. With the goal of diminishing the impacts of factors such as the sensors’ drift and devices, the innovative aspect of this method is to employ static accuracy for comparison. If the dynamic results are as good as the static results, which have accuracy comparable to the single star sensor’s precision, the practical precision of the star sensor is sufficiently high to meet the requirements of the system specification. The experiments demonstrate the feasibility and effectiveness of the proposed method.
Evidence for Rapid Oxidative Phosphorylation and Lactate Fermentation in Motile Human Sperm by Hyperpolarized 13C Magnetic Resonance Spectroscopy
Authors: Reynolds Steven , Ismail Nurul Fadhlina bt , Calvert Sarah J. , Pacey Allan A. , Paley Martyn N. J.
Published: June 2017

Poor sperm motility is a common cause of male infertility for which there are no empirical therapies. Sperm motility is powered by adenosine triphosphate but the relative importance of lactate fermentation and Oxidative Phosphorylation (OxPhos) is debated. To study the relationship between energy metabolism and sperm motility we used dissolution Dynamic Nuclear Polarization (dDNP) for the first time to show the rapid conversion of 13C1-pyruvate to lactate and bicarbonate, indicating active glycolytic and OxPhos metabolism in sperm. The magnitude of both lactate and bicarbonate signals were positively correlated with the concentration of progressively motile sperm. After controlling for sperm concentration, increased progressive sperm motility generated more pyruvate conversion to lactate and bicarbonate. The technique of dDNP allows ‘snapshots’ of sperm metabolism to be tracked over the different stages of their life. This may provide help to uncover the causes of poor sperm motility and suggest new approaches for novel treatments or therapies.
Live Imaging of Micro-Wettability Experiments Performed for Low-Permeability Oil Reservoirs
Authors: Deglint Hanford J. , Clarkson Christopher R. , DeBuhr Chris , Ghanizadeh Amin
Published: June 2017

Low-permeability (unconventional) hydrocarbon reservoirs exhibit a complex nanopore structure and micro (µm) -scale variability in composition which control fluid distribution, displacement and transport processes. Conventional methods for characterizing fluid-rock interaction are however typically performed at a macro (mm) -scale on rock sample surfaces. In this work, innovative methods for the quantification of micro-scale variations in wettability and fluid distribution in a low-permeability oil reservoir was enabled by using an environmental scanning electron microscope. Live imaging of controlled water condensation/evaporation experiments allowed micro-droplet contact angles to be evaluated, while imaging combined with x-ray mapping of cryogenically frozen samples facilitated the evaluation of oil and water micro-droplet contact angles after successive fluid injection. For the first time, live imaging of fluids injected through a micro-injection system has enabled quantification of sessile and dynamic micro-droplet contact angles. Application of these combined methods has revealed dramatic spatial changes in fluid contact angles at the micro-scale, calling into question the applicability of macro-scale observations of fluid-rock interaction.