bluuebunny/crossref_metadata_2025 huggingface dataset:
Industry 4.0: a bibliometric analysis of social partners’ public messages in France and Germany
Authors: Woźniak-Jęchorek Beata , Woźniak-Jasińska Katarzyna , Lewoniewski Włodzimierz , Kirov Vassil
Published: January 2024

This paper investigates how interest groups in France and Germany communicate information about Industry 4.0 technologies and approaches. Specifically, this explanatory study employs cutting-edge big-data-type tools and machine-based automatic text processing to delve into the topics, arguments, and postulates related to Industry 4.0 strategies by trade unions and employers’ organisations. The goal is to determine which of these factors have been pivotal in shaping social dialogue in France and Germany. The findings reveal that social partners in both countries are involved in similar digitalisation-related initiatives and express predominantly favourable viewpoints regarding Industry 4.0 technologies. Key themes in the dialogues of both France and Germany centre around workers’ rights, working conditions, and skills training.
Thermo-hydro-mechanical simulation of cooling-induced fault reactivation in Dutch geothermal reservoirs
Authors: Mathur Bakul , Hofmann Hannes , Cacace Mauro , Hutka Gergő András , Zang Arno
Published: January 2024

Geothermal energy is one of the most viable sources of renewable heat. However, the potential risk of induced seismicity associated with geothermal operations may slow down the growth of the geothermal sector. Previous research has led to significant progress in understanding fluid-injection-induced seismicity in geothermal reservoirs. However, an in-depth assessment of thermal effects on the seismic risk was generally considered to be of secondary importance. This study aims to investigate the relative influence of temperature and key geological and operational parameters on the slip tendency of pre-existing faults. This is done through coupled thermo-hydro-mechanical simulations of the injection and production processes in synthetic geothermal reservoir models of the most utilized and potentially exploitable Dutch geothermal reservoir formations: Slochteren sandstone, Delft sandstone and Dinantian limestone.

In our study, changes in the slip tendency of a fault can largely be attributed to thermo-elastic effects, which confirms the findings of recent studies linking thermal stresses to induced seismicity. While the direct pore pressure effect on slip tendency tends to dominate over the early phase of the operations, once pore pressure equilibrium is established in a doublet system, it is the additional stress change associated with the growing cold-water front around the injection well that has the greatest influence. Therefore, the most significant increase in the slip tendency was observed when this low-temperature front reached the fault zone. The distance between an injection well and a pre-existing fault thus plays a pivotal role in determining the mechanical stability of a fault. A careful selection of a suitable target formation together with an appropriate planning of the operational parameters is also crucial to mitigate the risk of induced seismicity. Besides the well-known relevance of the in situ stress field and local fault geometry, rock-mechanical properties and operation conditions exert a major influence on induced stress changes and therefore on the fault (re)activation potential during geothermal operations.
Effectiveness of a palliative care education program for caregivers of cancer patients receiving chemotherapy in Port Said City: A pre-post quasi-experimental study
Authors: Ibrahim Ateya Megahed , Elnaghy Sara Fawzy , Abo Elmatty Gehad Mohamed , Mohamed Ghida Nglaa Ibrahim , Mohamed Magda Ali
Published: January 2024

Background

Emphasizing the pivotal role of caregivers in the cancer care continuum, a program designed to educate caregivers of cancer patients undergoing chemotherapy underscores their significance. The palliative care education initiative strives to cultivate a compassionate and effective care environment, benefiting both patients and caregivers. By imparting education, fostering positive attitudes, offering support, encouraging appropriate behaviors, and providing essential resources, the program aims to enhance the overall caregiving experience and contribute to the well-being of those navigating the challenges of cancer treatment.

Objectives

To evaluate the effectiveness of a palliative care education program for caregivers of cancer patients receiving chemotherapy.

Methods

The research employed a purposive sample comprising 155 caregivers who were actively present with their cancer patients throughout the pre- and post-test phases within a quasi-experimental research design. The study took place at the outpatient oncology center of Al-Shifa Medical Complex in Port Said City, Egypt. To gather comprehensive data, 4 instruments were utilized: a demographic questionnaire, a nurse knowledge questionnaire, a scale measuring attitudes toward palliative care, and an assessment of reported practices in palliative care. This methodological approach allowed for a thorough exploration of caregiver perspectives, knowledge, attitudes, and practices within the context of a palliative care education program.

Results

Before the palliative care education program, only 1.3% of caregivers had a good overall level of knowledge about cancer and palliative care; this increased to 40.6% after the program. Similarly, before the palliative care education program, 32.9% of caregivers had a positive overall attitude, which increased to 72.3% after the program. Similarly, 27.1% of caregivers had an overall appropriate palliative care practice during the pre-test phase, which increased to 93.5% after the palliative care education program.

Significance of the results

The palliative care education program significantly improved caregivers’ knowledge, attitudes, and practice scores. It is strongly recommended that caregivers of cancer patients receive continuing education in palliative care. In addition, it is crucial to conduct further research with a larger sample size in different situations in Egypt.
Asymmetry of Submesoscale Instabilities in Anticyclonic and Cyclonic Eddies
Authors: Shi Weian , Lin Hongyang , Deng Qiang , Hu Jianyu
Published: January 2024

The upper‐ocean relative vorticity has been found to be cyclonically skewed, but altimetry observations indicate that long‐lifespan mesoscale eddies tend to be anticyclonic. We are thus interested in whether cyclonic or anticyclonic eddies are more unstable under similar circumstances. Here we use submesoscale‐resolving simulations of idealized mesoscale eddies, incorporating theoretical analyses, to investigate asymmetries of submesoscale instabilities within the anticyclones and cyclones. It is found that submesoscale filaments initiate at regions with the largest horizontal buoyancy gradients for both anticyclones and cyclones, but these filaments subsequently rotate outward in anticyclones while inward in cyclones. Hence submesoscales are more vigorous at anticyclone peripheries and the cyclone center. Such differing distributions and evolutions of submesoscale processes are primarily caused by changes in the background stratification associated with the decaying of mesoscale eddies. The active submesoscales near the cyclone center eventually distort its core structure radically, whereas the anticyclone remains largely unaffected.
Anticyclonic Suppression of the North Pacific Transient Eddy Activity in Midwinter
Authors: Okajima Satoru , Nakamura Hisashi , Kaspi Yohai
Published: January 2024

Dynamical understandings of midlatitude transient eddy activity, especially its midwinter minimum over the North Pacific, are still limited, partly because conventional Eulerian eddy statistics are incapable of separating cyclonic and anticyclonic contributions. Here we evaluate the two contributions separately based on local curvature of instantaneous flow fields to compare their seasonality between the North Pacific and North Atlantic storm‐tracks. The anticyclonic contribution is found crucial for the midwinter minimum of the North Pacific transient eddy activity. Eddy energetics reveals that the net efficiency of the anticyclonic contribution in replenishing total transient eddy energy over the North Pacific exhibits a pronounced midwinter minimum leading to net energy loss, while that of its cyclonic counterpart does not, in harmony with a precipitation peak around midwinter. This study suggests that more attention should be paid to anticyclones in studying midlatitude storm‐track dynamics.
Distinct Impacts of the Central and Eastern Atlantic Niño on the European Climate
Authors: Chen Baiyang , Zhang Lei , Wang Chunzai
Published: January 2024

The Atlantic Niño is the primary interannual variability mode in the tropical Atlantic, with far‐reaching impacts on global climate. A recent study identified two types of the Atlantic Niño, each with its maximum warming centered in the central and eastern equatorial Atlantic, respectively. Through analysis of observational data and numerical model experiments, we find that the two Atlantic Niño types have distinct climatic impacts on Europe. This is because the central Atlantic Niño is associated with a pronounced increase in precipitation in the western tropical Atlantic, while the positive precipitation anomalies during the eastern type are mainly located in the eastern basin with weaker amplitudes. Consequently, compared to the eastern Atlantic Niño, the extra‐tropical atmospheric waves and the associated precipitation and temperature anomalies in Europe during the central type are stronger and shifted westward. Therefore, distinguishing between the two Atlantic Niño types may help improve seasonal climate predictions in Europe.
Impacts of Gravity Waves on the Thermospheric Circulation and Composition
Authors: Liu H.‐L. , Lauritzen P. H. , Vitt F.
Published: January 2024

The high‐resolution Whole Atmosphere Community Climate Model with thermosphere/ionosphere extension (WACCM‐X) is used to study the impacts of gravity waves (GWs) on the thermospheric circulation and composition. The resolved GWs are found to propagate anisotropically with stronger eastward components at most altitudes. The dissipation of these waves in the thermosphere produces a net eastward forcing that reaches peak values between 200 and 250 km at mid‐high latitudes in both hemispheres. Consequently, the mean circulation is weakened in the winter hemisphere and enhanced in the summer, which in turn impacts the thermospheric composition. Most notably, the column integrated O/N2 in both hemispheres is reduced and agrees better with observations. The mean thermospheric GW forcing in the meridional direction has comparable amplitude and acts to modify the gradient‐wind relationship.
Why flying insects gather at artificial light
Authors: Fabian Samuel T. , Sondhi Yash , Allen Pablo E. , Theobald Jamie C. , Lin Huai-Ti
Published: January 2024

Explanations of why nocturnal insects fly erratically around fires and lamps have included theories of “lunar navigation” and “escape to the light”. However, without three-dimensional flight data to test them rigorously, the cause for this odd behaviour has remained unsolved. We employed high-resolution motion capture in the laboratory and stereo-videography in the field to reconstruct the 3D kinematics of insect flights around artificial lights. Contrary to the expectation of attraction, insects do not steer directly toward the light. Instead, insects turn their dorsum toward the light, generating flight bouts perpendicular to the source. Under natural sky light, tilting the dorsum towards the brightest visual hemisphere helps maintain proper flight attitude and control. Near artificial sources, however, this highly conserved dorsal-light-response can produce continuous steering around the light and trap an insect. Our guidance model demonstrates that this dorsal tilting is sufficient to create the seemingly erratic flight paths of insects near lights and is the most plausible model for why flying insects gather at artificial lights.
Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds
Authors: Wamhoff Eike-Christian , Ronsard Larance , Feldman Jared , Knappe Grant A. , Hauser Blake M. , Romanov Anna , Case James Brett , Sanapala Shilpa , Lam Evan C. , Denis Kerri J. St. , Boucau Julie , Barczak Amy K. , Balazs Alejandro B. , Diamond Michael S. , Schmidt Aaron G. , Lingwood Daniel , Bathe Mark
Published: January 2024

Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs are thymus-dependent antigens that are themselves immunogenic and can induce B cell responses that may neutralize the platform. Here, we investigate thymus-independent DNA origami as an alternative material for multivalent antigen display using the receptor binding domain (RBD) of the SARS-CoV-2 spike protein, the primary target of neutralizing antibody responses. Sequential immunization of mice with DNA-based VLPs (DNA-VLPs) elicits protective neutralizing antibodies to SARS-CoV-2 in a manner that depends on the valency of the antigen displayed and on T cell help. Importantly, the immune sera do not contain boosted, class-switched antibodies against the DNA scaffold, in contrast to P-VLPs that elicit strong B cell memory against both the target antigen and the scaffold. Thus, DNA-VLPs enhance target antigen immunogenicity without generating scaffold-directed immunity and thereby offer an important alternative material for particulate vaccine design.
Electron scale coherent structure as micro accelerator in the Earth’s magnetosheath
Authors: Xie Zi-Kang , Zong Qiu-Gang , Yue Chao , Zhou Xu-Zhi , Liu Zhi-Yang , He Jian-Sen , Hao Yi-Xin , Ng Chung-Sang , Zhang Hui , Yao Shu-Tao , Pollock Craig , Le Guan , Ergun Robert , Lindqvist Per-Arne
Published: January 2024

Turbulent energy dissipation is a fundamental process in plasma physics that has not been settled. It is generally believed that the turbulent energy is dissipated at electron scales leading to electron energization in magnetized plasmas. Here, we propose a micro accelerator which could transform electrons from isotropic distribution to trapped, and then to stream (Strahl) distribution. From the MMS observations of an electron-scale coherent structure in the dayside magnetosheath, we identify an electron flux enhancement region in this structure collocated with an increase of magnetic field strength, which is also closely associated with a non-zero parallel electric field. We propose a trapping model considering a field-aligned electric potential together with the mirror force. The results are consistent with the observed electron fluxes from ~50 eV to ~200 eV. It further demonstrates that bidirectional electron jets can be formed by the hourglass-like magnetic configuration of the structure.
Sex dependence of opioid-mediated responses to subanesthetic ketamine in rats
Authors: Di Ianni Tommaso , Ewbank Sedona N. , Levinstein Marjorie R. , Azadian Matine M. , Budinich Reece C. , Michaelides Michael , Airan Raag D.
Published: January 2024

Subanesthetic ketamine is increasingly used for the treatment of varied psychiatric conditions, both on- and off-label. While it is commonly classified as an N-methyl D-aspartate receptor (NMDAR) antagonist, our picture of ketamine’s mechanistic underpinnings is incomplete. Recent clinical evidence has indicated, controversially, that a component of the efficacy of subanesthetic ketamine may be opioid dependent. Using pharmacological functional ultrasound imaging in rats, we found that blocking opioid receptors suppressed neurophysiologic changes evoked by ketamine, but not by a more selective NMDAR antagonist, in limbic regions implicated in the pathophysiology of depression and in reward processing. Importantly, this opioid-dependent response was strongly sex-dependent, as it was not evident in female subjects and was fully reversed by surgical removal of the male gonads. We observed similar sex-dependent effects of opioid blockade affecting ketamine-evoked postsynaptic density and behavioral sensitization, as well as in opioid blockade-induced changes in opioid receptor density. Together, these results underscore the potential for ketamine to induce its affective responses via opioid signaling, and indicate that this opioid dependence may be strongly influenced by subject sex. These factors should be more directly assessed in future clinical trials.
Observing growth and interfacial dynamics of nanocrystalline ice in thin amorphous ice films
Authors: Lee Minyoung , Lee Sang Yup , Kang Min-Ho , Won Tae Kyung , Kang Sungsu , Kim Joodeok , Park Jungwon , Ahn Dong June
Published: January 2024

Ice crystals at low temperatures exhibit structural polymorphs including hexagonal ice, cubic ice, or a hetero-crystalline mixture of the two phases. Despite the significant implications of structure-dependent roles of ice, mechanisms behind the growths of each polymorph have been difficult to access quantitatively. Using in-situ cryo-electron microscopy and computational ice-dynamics simulations, we directly observe crystalline ice growth in an amorphous ice film of nanoscale thickness, which exhibits three-dimensional ice nucleation and subsequent two-dimensional ice growth. We reveal that nanoscale ice crystals exhibit polymorph-dependent growth kinetics, while hetero-crystalline ice exhibits anisotropic growth, with accelerated growth occurring at the prismatic planes. Fast-growing facets are associated with low-density interfaces that possess higher surface energy, driving tetrahedral ordering of interfacial H2O molecules and accelerating ice growth. These findings, based on nanoscale observations, improve our understanding on early stages of ice formation and mechanistic roles of the ice interface.
Q-RBSA: high-resolution 3D EBSD map generation using an efficient quaternion transformer network
Authors: Jangid Devendra K. , Brodnik Neal R. , Echlin McLean P. , Gudavalli Chandrakanth , Levenson Connor , Pollock Tresa M. , Daly Samantha H. , Manjunath B. S.
Published: January 2024

Gathering 3D material microstructural information is time-consuming, expensive, and energy-intensive. Acquisition of 3D data has been accelerated by developments in serial sectioning instrument capabilities; however, for crystallographic information, the electron backscatter diffraction (EBSD) imaging modality remains rate limiting. We propose a physics-based efficient deep learning framework to reduce the time and cost of collecting 3D EBSD maps. Our framework uses a quaternion residual block self-attention network (QRBSA) to generate high-resolution 3D EBSD maps from sparsely sectioned EBSD maps. In QRBSA, quaternion-valued convolution effectively learns local relations in orientation space, while self-attention in the quaternion domain captures long-range correlations. We apply our framework to 3D data collected from commercially relevant titanium alloys, showing both qualitatively and quantitatively that our method can predict missing samples (EBSD information between sparsely sectioned mapping points) as compared to high-resolution ground truth 3D EBSD maps.
Superconductivity in unconventional metals
Authors: Yang Zhilong , Sheng Haohao , Guo Zhaopeng , Zhang Ruihan , Wu Quansheng , Weng Hongming , Fang Zhong , Wang Zhijun
Published: January 2024

Based on first-principles calculations, we demonstrate that 1H/2H-phase transition metal dichalcogenidesMX2 (M = Nb, Ta; X = S, Se, Te) are unconventional metals, which have an empty-site band of$${A}_{1}^{{\prime} }@1e$$A1′@1eelementary band representation at the Fermi level. The computed phonon dispersions indicate the stability of the system at high temperatures, while the presence of the soft phonon mode suggests a phase transition to the charge density wave state at low temperatures. Based on the Bardeen-Cooper-Schrieffer theory and computed electron-phonon coupling, our calculations show that the superconductivity (SC) in NbSe2is mainly attributed to the soft phonon mode due to the half filling of the empty-site band. Accordingly, the SC has been predicted in unconventional metals TaNS monolayer and 2H-TaN2bulk with computedTC = 10 K and 26 K respectively. These results demonstrate that the unconventional metals with partial filling of the empty-site band offer an attractive platform to search for superconductors.
An 800-year record of benthic foraminifer images and 2D morphometrics from the Santa Barbara Basin
Authors: Kahanamoku-Meyer Sara S. , Samuels-Fair Maya , Kamel Sarah M. , Stewart Da’shaun , Wu Bryan , Kahn Leah X. , Titcomb Max , Mei Yingyan Alyssa , Bridge R. Cheyenne , Li Yuerong Sophie , Sinco Carolina , Moreno Julissa , Epino Josef T. , Gonzalez-Marin Gerson , Latt Chloe , Fergus Heather , Duijnstee Ivo A. P. , Finnegan Seth
Published: January 2024

The Santa Barbara Basin is an extraordinary archive of environmental and ecological change, where varved sediments preserve microfossils that provide an annual to decadal record of the dynamics of surrounding ecosystems. Of the microfossils preserved in these sediments, benthic foraminifera are the most abundant seafloor-dwelling organisms. While they have been extensively utilized for geochemical and paleoceanographic work, studies of their morphology are lacking. Here we use a high-throughput imaging method (AutoMorph) designed to extract 2D data from photographic images of fossils to produce a large image and 2D shape dataset of recent benthic foraminifera from two core records sampled from the center of the Santa Barbara Basin that span an ~800-year-long interval during the Common Era (1249–2008 CE). Information on more than 36,000 objects is included, of which more than 22,000 are complete or partially-damaged benthic foraminifera. The dataset also includes other biogenic microfossils including ostracods, pteropods, diatoms, radiolarians, fish teeth, and shark dermal denticles. We describe our sample preparation, imaging, and identification techniques, and outline potential data uses.
An extensive quantitative analysis of the effects of errors in beat-to-beat intervals on all commonly used HRV parameters
Authors: Rohr Maurice , Tarvainen Mika , Miri Seyedsadra , Güney Gökhan , Vehkaoja Antti , Hoog Antink Christoph
Published: January 2024

Heart rate variability (HRV) analysis is often used to estimate human health and fitness status. More specifically, a range of parameters that express the variability in beat-to-beat intervals are calculated from electrocardiogram beat detections. Since beat detection may yield erroneous interval data, these errors travel through the processing chain and may result in misleading parameter values that can lead to incorrect conclusions. In this study, we utilized Monte Carlo simulation on real data, Kolmogorov–Smirnov tests and Bland–Altman analysis to carry out extensive analysis of the noise sensitivity of different HRV parameters. The used noise models consider Gaussian and student-t distributed noise. As a result we observed that commonly used HRV parameters (e.g. pNN50 and LF/HF ratio) are especially sensitive to noise and that all parameters show biases to some extent. We conclude that researchers should be careful when reporting different HRV parameters, consider the distributions in addition to mean values, and consider reference data if applicable. The analysis of HRV parameter sensitivity to noise and resulting biases presented in this work generalizes over a wide population and can serve as a reference and thus provide a basis for the decision about which HRV parameters to choose under similar conditions.
Effects of stepwise administration of osteoprotegerin and parathyroid hormone-related peptide DNA vectors on bone formation in ovariectomized rat model
Authors: Eom Ye Ji , Kim Jang-Woon , Rim Yeri Alice , Lim Jooyoung , Jung Se In , Ju Ji Hyeon
Published: January 2024

Osteoporosis is a metabolic bone disease that impairs bone mineral density, microarchitecture, and strength. It requires continuous management, and further research into new treatment options is necessary. Osteoprotegerin (OPG) inhibits bone resorption and osteoclast activity. The objective of this study was to investigate the effects of stepwise administration of OPG-encoded minicircles (mcOPG) and a bone formation regulator, parathyroid hormone-related peptide (PTHrP)-encoded minicircles (mcPTHrP) in osteoporosis. The combined treatment with mcOPG and mcPTHrP significantly increased osteogenic marker expression in osteoblast differentiation compared with the single treatment groups. A model of postmenopausal osteoporosis was established in 12-week-old female rats through ovariectomy (OVX). After 8 weeks of OVX, mcOPG (80 µg/kg) was administered via intravenous injection. After 16 weeks of OVX, mcPTHrP (80 µg/kg) was injected once a week for 3 weeks. The bone microstructure in the femur was evaluated 24 weeks after OVX using micro-CT. In a proof-of-concept study, stepwise treatment with mcOPG and mcPTHrP on an OVX rat model significantly improved bone microstructure compared to treatment with mcOPG or mcPTHrP alone. These results suggest that stepwise treatment with mcOPG and mcPTHrP may be a potential treatment for osteoporosis.
Effects of birch encroachment, water table and vegetation on methane emissions from peatland microforms in a rewetted bog
Authors: Welpelo Carla , Dubbert Maren , Tiemeyer Bärbel , Voigt Claas , Piayda Arndt
Published: January 2024

This study investigated the influence of vegetation and microforms on methane (CH4) balances of a rewetted bog in north-west Germany. The two study sites are in close proximity on the same former peat extraction area, one dominated by Sphagnum-mosses and the other one by a dense Betula pubescens stand with a high Eriophorum vaginatum cover. The contribution of microforms (hummocks/hollows) to CH4 emissions and the effect of Betula encroachment has been studied. Transparent and opaque chambers were used to measure CH4 fluxes every 3–4 weeks during daytime for one year. For the estimation of annual balances, three methods were compared and the method using water level and soil temperature as explanatory variables was selected. Fluxes were scaled to the site level. The annual emissions per site are and 7.1 ± 1.5 g CH4-C m−2 year−1 at the treed site and 36.1 ± 3.5 g CH4-C m−2 year−1 at the open site, mainly controlled by higher water levels. Highest annual emissions originated from hollows at the open site, but in the vegetation period, hummock emissions tend to be higher. At the tree site, emission differences between the microforms were less pronounced. There were no differences between fluxes from transparent and opaque chambers.
Insights from a model based study on optimizing non invasive brain electrical stimulation for Parkinson’s disease
Authors: Sousani Maryam , Seydnejad Saeid R. , Ghahramani Maryam
Published: January 2024

Parkinson’s Disease (PD) is a disorder in the central nervous system which includes symptoms such as tremor, rigidity, and Bradykinesia. Deep brain stimulation (DBS) is the most effective method to treat PD motor symptoms especially when the patient is not responsive to other treatments. However, its invasiveness and high risk, involving electrode implantation in the Basal Ganglia (BG), prompt recent research to emphasize non-invasive Transcranial Electrical Stimulation (TES). TES proves to be effective in treating some PD symptoms with inherent safety and no associated risks. This study explores the potential of using TES, to modify the firing pattern of cells in BG that are responsible for motor symptoms in PD. The research employs a mathematical model of the BG to examine the impact of applying TES to the brain. This is conducted using a realistic head model incorporating the Finite Element Method (FEM). According to our findings, the firing pattern associated with Parkinson’s disease shifted towards a healthier firing pattern through the use of tACS. Employing an adaptive algorithm that continually monitored the behavior of BG cells (specifically, Globus Pallidus Pars externa (GPe)), we determined the optimal electrode number and placement to concentrate the current within the intended region. This resulted in a peak induced electric field of 1.9 v/m at the BG area. Our mathematical modeling together with precise finite element simulation of the brain and BG suggests that proposed method effectively mitigates Parkinsonian behavior in the BG cells. Furthermore, this approach ensures an improvement in the condition while adhering to all safety constraints associated with the current injection into the brain.
Bioinformatics analysis identifies coagulation factor II receptor as a potential biomarker in stomach adenocarcinoma
Authors: Wu Xingwei , Wang Shengnan , Wang Chenci , Wu Chengwei , Zhao Zhiyong
Published: January 2024

Coagulation factor 2 thrombin receptor (F2R), a member of the G protein-coupled receptor family, plays an important role in regulating blood clotting through protein hydrolytic cleavage mediated receptor activation. However, the underlying biological mechanisms by which F2R affects the development of gastric adenocarcinoma are not fully understood. This study aimed to systematically analyze the role of F2R in gastric adenocarcinoma. Stomach adenocarcinoma (STAD)-related gene microarray data and corresponding clinicopathological information were downloaded from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Differential expression genes (DEGs) associated with F2R were analyzed using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), gene set enrichment analysis (GSEA), and protein–protein interaction (PPI) networks. F2R mRNA expression data were utilized to estimate stromal cell and immune cell scores in gastric cancer tissue samples, including stromal score, immune score, and ESTIMATE score, derived from single-sample enrichment studies. Analysis of TCGA and GEO databases revealed significantly higher F2R expression in STAD tissues compared to normal tissues. Patients with high F2R expression had shorter survival times than those with low F2R expression. F2R expression was significantly correlated with tumor (T) stage, node (N) stage, histological grade and pathological stage. Enrichment analysis of F2R-related genes showed that GO terms were mainly related to circulation-mediated human immune response, immunoglobulin, cell recognition and phagocytosis. KEGG analysis indicated associations to extracellular matrix (ECM) receptor interactions, neuroactive ligand-receptor interactions, the phosphoinositide-3-kinase-protein kinase B/Akt (PI3K-AKT) signaling pathway, the Wnt signaling pathway and the transforming growth factor-beta (TGF-β) signaling pathway. GSEA revealed connections to DNA replication, the Janus kinase/signal transducers and activators of transcription (JAK-STAT) signaling pathway, the mitogen-activated protein kinase (MAPK) signaling pathway and oxidative phosphorylation. Drug sensitivity analysis demonstrated positive correlations between F2R and several drugs, including BEZ235, CGP-60474, Dasatinib, HG-6-64-1, Aazopanib, Rapamycin, Sunitinib and TGX221, while negative correlation with CP724714, FH535, GSK1904529A, JNK-9L, LY317615, pyrimidine, rTRAIL and Vinorelbine. Knocking down F2R in GC cell lines resulted in slowed proliferation, migration, and invasion. All statistical analyses were performed using R software (version 4.2.1) and GraphPad Prism 9.0. p < 0.05 was considered statistically significant. In conclusion, this study underscores the significance of F2R as a potential biomarker in gastric adenocarcinoma, shedding light on its molecular mechanisms in tumorigenesis. F2R holds promise for aiding in the diagnosis, prognosis, and targeted therapy of STAD.
The role of intimate partner violence perpetrators' resting state functional connectivity in treatment compliance and recidivism
Authors: Romero-Martínez Ángel , Beser María , Cerdá-Alberich Leonor , Aparici Fernando , Martí-Bonmatí Luis , Sarrate-Costa Carolina , Lila Marisol , Moya-Albiol Luis
Published: January 2024

To expand the scientific literature on how resting state functional connectivity (rsFC) magnetic resonance imaging (MRI) (or the measurement of the strength of the coactivation of two brain regions over a sustained period of time) can be used to explain treatment compliance and recidivism among intimate partner violence (IPV) perpetrators. Therefore, our first aim was to assess whether men convicted of IPV (n = 53) presented different rsFC patterns from a control group of non-violent (n = 47) men. We also analyzed if the rsFC of IPV perpetrators before staring the intervention program could explain treatment compliance and recidivism one year after the intervention ended. The rsFC was measured by applying a whole brain analysis during a resting period, which lasted 45 min. IPV perpetrators showed higher rsFC in the occipital brain areas compared to controls. Furthermore, there was a positive association between the occipital pole (OP) and temporal lobes (ITG) and a negative association between the occipital (e.g., occipital fusiform gyrus, visual network) and both the parietal lobe regions (e.g., supramarginal gyrus, parietal operculum cortex, lingual gyrus) and the putamen in IPV perpetrators. This pattern was the opposite in the control group. The positive association between many of these occipital regions and the parietal, frontal, and temporal regions explained treatment compliance. Conversely, treatment compliance was also explained by a reduced rsFC between the rostral prefrontal cortex and the frontal gyrus and both the occipital and temporal gyrus, and between the temporal and the occipital and cerebellum areas and the sensorimotor superior networks. Last, the enhanced rsFC between the occipital regions and both the cerebellum and temporal gyrus predicted recidivism. Our results highlight that there are specific rsFC patterns that can distinguish IPV perpetrators from controls. These rsFC patterns could be useful to explain treatment compliance and recidivism among IPV perpetrators.
Tailoring of an anti-diabetic drug empagliflozin onto zinc oxide nanoparticles: characterization and in vitro evaluation of anti-hyperglycemic potential
Authors: Shoaib Abdullah , Shahid Sammia , Mansoor Sana , Javed Mohsin , Iqbal Shahid , Mahmood Sajid , Bahadur Ali , Jaber Fadi , Alshalwi Matar
Published: January 2024

Diabetes is a serious health issue that can be a great risk factor related to numerous physical problems. A class of drugs “Gliflozin” especially Sodium Glucose Co. Transporter 2 was inhibited by a novel drug, which is known as “empagliflozin”. While ZnO nanoparticles (NPs) had considerable promise for combating diabetes, it was employed in the treatment and management of type-2 diabetes mellitus. The new drug empagliflozin was initially incorporated into Zinc Oxide NPs in this study using the surface physio-sorption technique, and the degree of drug adsorption was assessed using the HPLC method. The tailored product was characterized by using the FTIR, EDX, Ultraviolet–Visible, XRD and SEM techniques. With an average particle size of 17 nm, SEM revealed mono-dispersion of NPs and sphere-like form. The Freundlich isotherm model best fits and explains the data for the physio-sorption investigation, which examined adsorption capabilities using adsorption isotherms. The enzymes α-amylase and α-glucosidase, which are involved in the human metabolism of carbohydrates, were used in the in-vitro anti-diabetic assays. It was discovered that the composite showed the highest levels of 81.72 and 92.77% inhibition of -α-amylase and -glucosidase at an absolute concentration of 1000 μg per ml with IC50 values of 30.6 μg per ml and 72 μg per ml.
The podocytes’ inflammatory responses in experimental GN are independent of canonical MYD88-dependent toll-like receptor signaling
Authors: Schömig Thomas , Diefenhardt Paul , Plagmann Ingo , Trinsch Bastian , Merz Tim , Crispatzu Giuliano , Unnersjö-Jess David , Nies Jasper , Pütz David , Sierra Gonzalez Claudio , Schermer Bernhard , Benzing Thomas , Brinkkoetter Paul Thomas , Brähler Sebastian
Published: January 2024

Podocytes form the kidney filtration barrier and continuously adjust to external stimuli to preserve their integrity even in the presence of inflammation. It was suggested that canonical toll-like receptor signaling, mediated by the adaptor protein MYD88, plays a crucial role in initiating inflammatory responses in glomerulonephritis (GN). We explored the influence of podocyte-intrinsic MYD88 by challenging wild-type (WT) and podocyte-specific Myd88 knockout (MyD88pko) mice, with a model of experimental GN (nephrotoxic nephritis, NTN). Next-generation sequencing revealed a robust upregulation of inflammatory pathways and changes in cytoskeletal and cell adhesion proteins in sorted podocytes from WT mice during disease. Unchallenged MyD88pko mice were healthy and showed no proteinuria, normal kidney function and lacked morphological changes. During NTN, MyD88pko exhibited a transient increase in proteinuria in comparison to littermates, while histological damage, podocyte ultrastructure in STED imaging and frequencies of infiltrating immune cells by flow cytometry were unchanged. MYD88-deficiency led to subtle changes in the podocyte transcriptome, without a significant impact on the overall podocyte response to inflammation, presumably through MYD88-independent signaling pathways. In conclusion, our study reveals a comprehensive analysis of podocyte adaptation to an inflammatory environment on the transcriptome level, while MYD88-deficiency had only limited impact on the course of GN suggesting additional signaling through MYD88-independent signaling.
A distinct and reproducible teleconnection pattern over North America during extreme El Niño events
Authors: Beniche Margot , Vialard Jérôme , Lengaigne Matthieu , Voldoire Aurore , Srinivas Gangiredla , Hall Nicholas M. J.
Published: January 2024

El Niño-Southern Oscillation (ENSO) teleconnections are an important predictability source for extratropical seasonal climate forecasts. Previous studies suggest that the ENSO teleconnection pattern depends on the ENSO phase (El Niño vs. La Niña) and/or Sea Surface Temperature (SST) pattern (central Pacific vs. eastern Pacific El Niño events). Observations and ensemble simulations with the CNRM-CM6.1 atmospheric general circulation model indicate that only extreme El Niño events (e.g. 1982–1983, 1997–1998, 2015–2016) display a statistically significant eastward shift relative to the well-known Pacific-North American teleconnection pattern that occurs during both central and eastern Pacific moderate El Niño or during La Niña. This specific teleconnection pattern emerges when equatorial SST anomalies are both eastward-shifted and sufficiently large to exceed the deep atmospheric convection threshold over most of the eastern Pacific, resulting in a basin-wide reorganization of tropospheric heat sources. It yields> 0.5 std wet conditions over Western United States (74% likelihood) as well as> 0.5 std warm anomalies over Canada and the Northern United States (71% likelihood), with more consistency across events and ensemble members than for any other El Niño or La Niña type. These findings hold important implications for the seasonal forecasting of El Niño’s impacts on the North American climate.
Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA
Authors: Smarzewska Sylwia , Ignaczak Anna , Koszelska Kamila
Published: January 2024

In this study, electrochemical and theoretical studies were performed to explain the interaction mechanism between ponatinib (PNT), a third generation tyrosine kinase inhibitor, and dsDNA. The electrochemical part was conducted in phosphate-buffered saline (PBS) at physiological pH of 7.4 and in acetate buffer with a pH of 4.7, using square wave voltammetry. A boron-doped diamond electrode was used in a bulk-incubated solution. The theoretical part was investigated using computational methods, such as the semiempirical method PM7 and density functional theory (DFT). Significant differences in the electrochemical behavior of PNT in the presence of DNA confirmed the occurrence of interactions. The results obtained in the acetate buffer strongly suggested the preferential interaction of PNT with guanine residues. However, at physiological pH, it can be concluded that PNT interacts with dGua and dAdo in the dsDNA molecule. These results are consistent with outcomes from the theoretical studies, where quantum-chemical calculations showed that both electrochemically detectable nucleobases form hydrogen bonds with the drug. These bonds appeared to be stronger with guanine than with adenine. According to the computational studies, the dsDNA major groove is the energetically preferred site for the complexation of PNT.
Response of the modified GAFCHROMIC EBT2 radiochromic film to DC glow discharge plasma
Authors: Farag Omar F. , Kotb Omar M. , Ghazaly M. El , EL-Sayed Naglaa M.
Published: January 2024

The response of the modified GAFCHROMIC EBT2 radiochromic film to DC Oxygen glow discharge plasma was investigated using a flatbed scanner and an UV–Vis spectrophotometer. The film was modified by removing the polyester overlaminate, adhesive, and topcoat layers with a total thickness of 80 µm, and is now referred to as EBT2-M. The EBT2-M films were exposed to DC Oxygen plasma for different durations: 0, 0.5, 1, 2, 3, 4, 7, and 10 min. The exposed films exhibit coloration homogeneity with an average variation of (1.6 ± 0.3) × 10−4 pixel values/µm, irrespective of the applied exposure time. The pixel values of the red-and-green channels and weighted grayscale images decreased exponentially with different sensitivity amounts to $$\sim$$
∼
 39.67, 49.69, and 42.11 min−1, respectively, as the exposure time increased. The two absorption peaks at 580 ± 4 nm and 632 ± 4 nm in the UV–Vis absorption spectra of the exposed GAFCHROMIC EBT2-M radiochromic films are increasing with increasing exposure time up to 4 min, thereafter saturated for prolonged exposure time. The integrated absorbance in the range from 400 to 700 nm is linearly correlated with the exposure time. The indirect and direct optical energy band gaps and Urbach energy of the modified GAFCHROMIC EBT2 film are weakly correlated with the exposure time. These findings suggest the utilization of the modified GAFCHROMIC EBT2 radiochromic film as a novel and simple technique for plasma diagnostics.
Concurrent predictors of mathematics achievement for 9-year-old children with Williams syndrome
Authors: Guimaraes Vitor N. , Mervis Carolyn B.
Published: January 2024

Research on mathematics achievement by children with Williams syndrome (WS) has been very limited. We describe the math achievement of 72 9-year-olds with WS, compare their math and reading achievement, and explore concurrent predictors of math achievement using the Wechsler Individual Achievement Test-III (WIAT-III) to measure achievement and the Differential Ability Scales-II (DAS-II) to measure cognitive abilities. For both Numerical Operations and Math Problem Solving, mean standard scores (SSs) were in the mild disability range with a full range from severe disability to average ability. Bayesian robust estimation indicated decisive evidence that average reading performance was higher than average math performance. Bayesian multiple linear regression models with informative priors accounted for 60% of the variance in Numerical Operations SS with decisive evidence supporting an effect of Working Memory SS and strong evidence for Nonverbal Reasoning SS and Spatial SS and 71% of the variance in Math Problem Solving SS with decisive evidence supporting effects of Working Memory and Nonverbal Reasoning SS, strong evidence for Verbal SS, and substantial evidence for Spatial SS. These predictors are consistent with those for typically developing individuals, supporting consideration of interventions that have been highly effective for children with math difficulties in the general population.
RARRES2 is involved in the “lock-and-key” interactions between osteosarcoma stem cells and tumor-associated macrophages
Authors: Ma Jingjin , Chen Zhiyu , Li Qiaochu , Wang Linbang , Chen Jiaxing , Yang Xinyu , Yang Chaohua , Quan Zhengxue
Published: January 2024

Osteosarcoma (OS) is a type of tumor. Osteosarcoma stem cells (OSCs) are responsible for drug resistance, recurrence, and immunosuppression in OS. We aimed to determine the heterogeneity of OSCs and the immunosuppression mechanisms underlying the interactions between OSCs and tumor-associated macrophages (TAMs). The cell components, trajectory changes, and cell communication profiles of OS cells were analyzed by transcriptomics at the single-cell level. The intercellular communication patterns of OSCs were verified, and the role of the cell hub genes was revealed. Hub geneS are genes that play important roles in regulating certain biological processes; they are often defined as the genes with the strongest regulatory effect on differentially expressed gene sets. Moreover, various cellular components of the OS microenvironment were identified. Malignant cells were grouped, and OSCs were identified. Further regrouping and communication analysis revealed that the genes in the stemness maintenance and differentiation subgroups were involved in communication with macrophages. Key receptor–ligand pairs and target gene sets for cell communication were obtained. Transcriptome data analysis revealed the key gene RARRES2, which is involved in intercellular communication between OSCs and TAMs. In vitro studies confirmed that macrophages promote RARRES2-mediated stemness maintenance in OSCs via the TAM-secreted cytokine insulin-like growth factor 1. Patient studies confirmed that RARRES2 could be a biomarker of OS. OSCs are highly heterogeneous, and different subgroups are responsible for proliferation and communication with other cells. The IGF-RARRES2 axis plays a key role in maintaining OSC stemness through communication with TAMs.
Limited ability of increased sequencing depth in detecting cases missed by noninvasive prenatal testing: a comparative analysis of 3 clinical cases
Authors: Lu Yinghong , Zuo Na , Ning Minxia , Xie Yuling , Liu Weiwu , Ning Sisi , Liang Yi , Chen Xiao , Zhang Yuping , Feng Jun , Qin Yunrong
Published: January 2024

Increased sequencing depth can improve the detection rate of noninvasive prenatal testing (NIPT) for chromosome aneuploidies and copy number variations (CNVs). However, due to the technical limitations of NIPT, false-positives and false-negatives are inevitable. False-positives for aneuploidy and CNVs have been widely reported, but few missed cases have been reported. In this study, we report 3 patients missed by NIPT, which were still missed after increasing the sequencing depth. To verify the detection efficiency of the platform, the results of NIPT in 32,796 patients treated in Yulin Women and Children Health Care Hospital from 2020 to 2022 were retrospectively analyzed. Data on false-negative cases found by postnatal follow-up or amniocentesis were collected, and the sequencing data, pregnancy examination data, and postnatal follow-up results of these missed patients were summarized. Five patients missed by NIPT were found, and they were missed again by retesting or increasing the sequencing depth. Except for hypospadias found in 1 patient, ultrasonography of the other 4 patients showed no obvious abnormalities during the whole pregnancy. Our results suggest that pregnant women should be fully informed of the benefits and limitations of NIPT before undergoing the examination to avoid unnecessary medical disputes.