bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Environmental Policy Mix on Carbon Emission Reduction and Social Welfare: Scenario Simulation Based on Private Vehicle Trajectory Big Data
Authors: Chen Wenjie , Wu Xiaogang , Xiao Zhu
Published: August 2023

Analyzing and investigating the impact of implementing an environmental policy mix on carbon emission from private cars and social welfare holds significant reference value. Firstly, based on vehicle trajectory big data, this paper employs reverse geocoding and artificial neural network models to predict carbon emissions from private cars in various provinces and cities in China. Secondly, by simulating different scenarios of carbon tax, carbon trading, and their policy mix, the propensity score matching model is constructed to explore the effects of the policy mix on carbon emission reduction from private cars and social welfare while conducting regional heterogeneity analysis. Finally, policy proposals are proposed to promote carbon emission reduction from private cars and enhance social welfare in China. The results indicate that the environmental policy mix has a significant positive impact on carbon emission reduction from private cars and social welfare. Furthermore, in the regional heterogeneity analysis, the implementation of the policy mix in eastern regions has a significant positive effect on both carbon emission reduction from private cars and social welfare, while in central and western regions, it shows a significant positive impact on social welfare but has no significant effect on carbon emission reduction in the private car sector.
Preparation and Characterization of a Novel Artemisia Oil Packaging Film and Its Application in Mango Preservation
Authors: Meng Xiaohan , Lv Ze , Jiang Tianzhen , Tan Yifei , Sun Shaoyang , Feng Jianguo
Published: August 2023

In this work, a new food packaging film was synthesized via blending Artemisia oil (AO) into soybean protein isolate (SPI) and gelatin (Gel) for the postharvest storage of mango. The morphological architecture and mechanical properties of the films were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometer (XRD), and other technologies. The results show that the prepared films had relatively flat surfaces with good mechanical properties. AO enhanced the light-blocking ability of the film, increased the hydrophobicity, and affected the moisture content and water solubility of the film to a certain extent. Furthermore, the antioxidant performance and antifungal (Colletotrichum gloeosporioides) capacity of the films increased with higher AO concentration due to the presence of the active components contained in AO. During mango storage applications, the films showed good freshness retention properties. The above results indicate that SPI–Gel films containing AO have excellent physicochemical and application properties and have great potential in the field of food packaging.
Effect of Calcium Salts on the Firmness and Physicochemical and Sensorial Properties of Iranian Black Olive Cultivars
Authors: Ataollahi Eshkour Mahnaz , Ghorbani-HasanSaraei Azade , Rafe Ali , Shahidi Seyed-Ahmad , Naghizadeh Raeisi Shahram
Published: August 2023

Black olive has become one of the most prestigious olives processed in the olive industry, and its processing has been increased recently in different countries. The firmness of black olives may be changed by the processing methods, fermentation, and solution salts. In this study, the employment of CaCl2, Ca-acetate, and Ca-lactate during the processing of some Iranian black olive cultivars, including Mari, Zard, Rowghani, Shengeh, Dakal, Dezful, and Fishomi, was evaluated in terms of physicochemical and phenolic compounds and textural attributes. The results showed that Ca-lactate improved the firmness of the Mari cultivar from 1455 to 1765 N/100 g in the pitted olive, and the same trend was obtained for the other cultivars. Ca-acetate improved the black shiny color of the Mari cultivar from 4.36 to 4.85 and the sensorial properties of the black olives, including gustatory and kinesthetic sensations, were improved by using a Ca-lactate solution. The application of calcium salts in the salt-free preservation solutions imparted neither bitterness to the olives nor discoloration. The highest amounts of acid (1.42–1.56%), fructose to mannitol ratio (1–1.2), and phenolic compounds (955–963 mg/kg) were found for the Zard cultivar. Furthermore, the residual content of oleuropein was higher when CaCl2 was employed (357 mg/kg). All of the calcium salts improved the firmness of the black olives, although the maximum firmness was observed for the Ca-lactate. Consequently, the formation of a black shiny color is related to the diffusion of phenolic compounds; however, this needs further investigation to determine which kind of phenolic compound is responsible for its black color.
Baseline Profiles of Drug Prescriptions Prior to Diagnosis of Mild Cognitive Impairment (MCI) Obtained by Latent Class Analysis (LCA), and Assessment of Their Association with Conversion to Dementia
Authors: Gómez-Gómez Carmen , Moya-Molina Miguel Ángel , Tey-Aguilera Manuel Jesús , Flores-Azofra Jorge , González-Caballero Juan Luis
Published: August 2023

Polypharmacy has been linked to cognitive decline. However, interventions targeting modifiable risk factors, some of which are targets of the most commonly used drugs, could reduce the prevalence of dementia. Our aim was to determine the drug prescription regimen at baseline, prior to the diagnosis of mild cognitive impairment (MCI), and its possible association with progression to dementia. Data were collected from the electronic medical records of 342 MCI outpatients diagnosed during 2006–2017 at their first neurology consultation. We followed the classical three-step method of statistical analysis, starting with a Latent Class Analysis (LCA) to discover subgroups of drug prescription probability. Half of the patients were under polypharmacy (≥5 drugs), 17.5% had no recorded medication, 33.3% progressed to dementia (94.7% in ≤5 years), and 84.1% of them to Alzheimer’s disease (AD). According to the LCA and based on 20 therapeutic indicators obtained from 240 substances and regrouped according the Anatomical Therapeutic Chemical Classification, we identified a four-profile model: (1) low (35.7% of patients); (2) mixed (28.7%); (3) cardio-metabolic (19.3%); and (4) psychotropic (16.4%). The binomial regression logistic model showed that profiles 2 and 3 (and 4 for AD), with a higher drug prescription conditioned probability against classic risk factors, were protective than profile 1 (OR = 0.421, p = 0.004; OR = 0.278, p = 0.000; OR = 0.457, p = 0.040, respectively), despite polypharmacy being significant in profiles 2 and 3 (mean > 7 drugs) vs. profile 1 (1.4 ± 1.6) (p = 0.000). Patients in the latter group were not significantly older, although being aged 65–79 years old quadrupled (OR = 4.217, p = 000) and being >79 tripled (OR = 2.945, p = 0.010) the conversion risk compared to patients <65 years old. According to the proposed analytical model, profiling the heterogeneous association of risk factors, which were taken prior to diagnosis, could be explored as an indicator of prior care and a predictor of conversion to dementia.
Quality Assessment and Modulating Factors on Self-Regulatory Behavior in Peer-Assisted Medical Learning
Authors: Achenbach Jannis , Nockelmann Laura , Thomas Michaela , Schäfer Thorsten
Published: August 2023

Objectives: Standardized extracurricular skills labs courses have been developed in recent decades and are important approaches in peer-assisted medical learning (PAL). To provide high quality training and achieve effective learning strategies, continuous evaluations and quality assessments are essential. This research aims to evaluate quality data from medical students participating in extracurricular skills labs courses at Ruhr-University Bochum to prospectively optimize concepts and didactical training and standardize processes. Additionally, we set out to assess and quantify drivers that are influencing factors of the self-reflection of competencies. Methods: The analysis was based on a routine assessment of n = 503 attendees of the PAL courses in the skills labs in three consecutive semesters, who voluntarily participated in the evaluation. We analyzed the effects of age, semester and their interaction on the self-reflection of competencies in technical skills courses using moderated regression and simple slope analyses, as previously published. A univariate analysis of variance (ANOVA) with post hoc Tukey HSD testing was used to analyze group means in estimated competencies using IBM SPSS Statistics V.28. Results: An analysis of variance revealed a significant increase in self-assessed competencies when comparing pre- vs. post-course evaluation data in all 35 depicted items (all p < 0.001). A total of 65.5% of the items were adjusted significantly differently, revealing modified self-reflected pre-course levels compared to those stated before. A moderated regression analysis revealed that age (R2 = 0.001, F(1;2347) = 1.88, p < 0.665), semester of study (∆R2 = 0.001, ∆F (1;2346) = 0.012, p < 0.912) and their interaction (∆R2 = 0.001, ∆F (1;2345) = 10.72, p < 0.227) did not explain a significant amount of the variance in self-reflection variance. A simple slope analysis of earlier (b = 0.07, t = 0.29, p < 0.773) and later semesters of study (b = 0.06, t = 0.07, p < 0.941) did not differentiate from zero. Conclusions: The presented evaluation paradigm proved to be a useful tool to encourage students to initiate self-regulatory and self-reflective behavior. The cohesive evaluation of the large cohort of attendees in extracurricular, facultative skills labs courses was helpful in terms of quality assessments and future adaptations. Further evaluation paradigms should be implemented to assess other influencing factors, such as gender, on self-reflection, since age and semester did not explain significant differences in the model.
Assessment of 3D Model for Photogrammetric Purposes Using AI Tools Based on NeRF Algorithm
Authors: Pepe Massimiliano , Alfio Vincenzo Saverio , Costantino Domenica
Published: August 2023

The aim of the paper is to analyse the performance of the Neural Radiance Field (NeRF) algorithm, implemented in Instant-NGP software, for photogrammetric purposes. To achieve this aim, several datasets with different characteristics were analysed, taking into account object size, image acquisition technique and geometric configuration of the images. The NeRF algorithm proved to be effective in the construction of the 3D models; in other words, in Instant-NGP it was possible to obtain realistic 3D models in a detailed manner and very quickly, even in rather weak geometric configurations of the images. The performance obtained in the latter environment was compared with that achieved by two software packages, one widely used in the photogrammetric field, Agisoft Metashape, and one open source, Colmap. The comparison showed encouraging results in building 3D models, especially under weak geometry conditions; although, the geometric description of objects under point clouds or meshes needs improvement for use in the photogrammetric field.
Molecular Mechanisms Underlying Chronic and Degenerative Diseases
Authors: Cannavo Alessandro
Published: August 2023

The Special Issue entitled “Molecular Mechanisms Underlying Chronic and Degenerative Diseases” contains eight articles: six original studies and two reviews [...]
Role of TRP Channels in Liver-Related Diseases
Authors: Liu Yusheng , Lyu Yihan , Zhu Lijuan , Wang Hongmei
Published: August 2023

The liver plays a crucial role in preserving the homeostasis of an entire organism by metabolizing both endogenous and exogenous substances, a process that relies on the harmonious interactions of hepatocytes, hepatic stellate cells (HSCs), Kupffer cells (KCs), and vascular endothelial cells (ECs). The disruption of the liver’s normal structure and function by diverse pathogenic factors imposes a significant healthcare burden. At present, most of the treatments for liver disease are palliative in nature, rather than curative or restorative. Transient receptor potential (TRP) channels, which are extensively expressed in the liver, play a crucial role in regulating intracellular cation concentration and serve as the origin or intermediary stage of certain signaling pathways that contribute to liver diseases. This review provides an overview of recent developments in liver disease research, as well as an examination of the expression and function of TRP channels in various liver cell types. Furthermore, we elucidate the molecular mechanism by which TRP channels mediate liver injury, liver fibrosis, and hepatocellular carcinoma (HCC). Ultimately, the present discourse delves into the current state of research and extant issues pertaining to the targeting of TRP channels in the treatment of liver diseases and other ailments. Despite the numerous obstacles encountered, TRP channels persist as an extremely important target for forthcoming clinical interventions aimed at treating liver diseases.
Selective Internal Radiotherapy Alters the Profiles of Systemic Extracellular Vesicles in Hepatocellular Carcinoma
Authors: Gylstorff Severin , Wilke Vanessa , Kraft Daniel , Bertrand Jessica , Pech Maciej , Haag Florian , Relja Borna
Published: August 2023

Incidence of hepatocellular carcinoma (HCC) is increasing globally. Radioembolization (RE)/selective internal radiotherapy (SIRT) is a promising treatment for inoperable HCC. RE triggers an immune response, involving extracellular vesicles (EVs) which are crucial for cell communication and tumor development. This study explores EV immune profiles and origins in patients with inoperable HCC before and after SIRT/RE. Blood samples from 50 HCC-patients treated with SIRT/RE were collected before and after therapy to determine cytokines and isolate EVs using size exclusion chromatography. The dynamic range and EV quality required for detecting variations in surface markers were assessed. Thirty-seven EV surface markers were analyzed using flow cytometry and correlated with clinical parameters. Several immunological markers (CD4, CD2, CD40, CD45, CD49e, CD69, CD209-EVs) were present in the circulation of HCC patients. These markers positively correlated with therapy response and survival. Conversely, B cell CD20, endothelial cell CD146, platelet CD49e, and CD41b EV markers negatively correlated with 60-day survival. Elevated levels of IL-6 and IL-8 before therapy correlated negatively with patient survival, coinciding with a positive correlation with CD20-positive EVs. Plasma EVs from HCC patients exhibit immunological, cancer, and coagulation markers, including potential biomarkers (CD4, CD20, CD49e, CD146). These may enhance our understanding of cancer biology and facilitate SIRT therapy monitoring.
Co-Existence of blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt
Authors: Sánchez-Urtaza Sandra , Ocampo-Sosa Alain , Molins-Bengoetxea Ainhoa , Rodríguez-Grande Jorge , El-Kholy Mohammed A. , Hernandez Marta , Abad David , Shawky Sherine M. , Alkorta Itziar , Gallego Lucia
Published: August 2023

The increasing rates of antimicrobial resistance among carbapenem-resistant Acinetobacter baumannii in the Middle East and North Africa are one of the major concerns for healthcare settings. We characterised the first A. baumannii isolate harbouring five β-lactamases identified in Egypt. The isolate Ale25 was obtained from an ICU patient of a hospital from Alexandria. The isolate was phenotypically and genotypically screened for carbapenemase genes. The isolate was resistant to carbapenems, aminoglycosides, fluoroquinolones and cefiderocol. Whole-Genome Sequencing identified five β-lactamase genes, blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57, together with other antibiotic resistance genes, conferring resistance to sulfonamides, macrolides, tetracyclines, rifamycin and chloramphenicol. Virulome analysis showed the presence of genes involved in adhesion and biofilm production, type II and VI secretion systems, exotoxins, etc. Multi-Locus Sequence Typing analysis identified the isolate as Sequence Types 113Pas and 2246Oxf, belonging to International Clone 7. Sequencing experiments revealed the presence of four plasmids of 2.7, 22.3, 70.4 and 240.8 Kb. All the β-lactamase genes were located in the chromosome, except the blaPER-7, gene which was found within the plasmid of 240.8 Kb. This study highlights the threat of the emergence and dissemination of these types of isolates.
Robust ParB Binding to Half-parS Sites in Pseudomonas aeruginosa—A Mechanism for Retaining ParB on the Nucleoid?
Authors: Kawalek Adam , Bartosik Aneta Agnieszka , Jagura-Burdzy Grazyna
Published: August 2023

Chromosome segregation in Pseudomonas aeruginosa is assisted by the tripartite ParAB–parS system, composed of an ATPase (ParA), a DNA-binding protein (ParB) and its target parS sequence(s). ParB forms a nucleoprotein complex around four parSs (parS1–parS4) that overlaps oriC and facilitates relocation of newly synthesized ori domains inside the cells by ParA. Remarkably, ParB of P. aeruginosa also binds to numerous heptanucleotides (half-parSs) scattered in the genome. Here, using chromatin immunoprecipitation-sequencing (ChIP-seq), we analyzed patterns of ParB genome occupancy in cells growing under conditions of coupling or uncoupling between replication and cell division processes. Interestingly, a dissipation of ParB–parS complexes and a shift of ParB to half-parSs were observed during the transition from the exponential to stationary phase of growth on rich medium, suggesting the role of half-parSs in retaining ParB on the nucleoid within non-dividing P. aeruginosa cells. The ChIP-seq analysis of strains expressing ParB variants unable to dislocate from parSs showed that the ParB spreading ability is not required for ParB binding to half-parSs. Finally, a P. aeruginosa strain with mutated 25 half-parSs of the highest affinity towards ParB was constructed and analyzed. It showed altered ParB coverage of the oriC region and moderate changes in gene expression. Overall, this study characterizes a novel aspect of conserved bacterial chromosome segregation machinery.
Promise and Challenges of T Cell Immunotherapy for Osteosarcoma
Authors: Park Jeong A , Cheung Nai-Kong V.
Published: August 2023

The cure rate for metastatic or relapsed osteosarcoma has not substantially improved over the past decades despite the exploitation of multimodal treatment approaches, allowing long-term survival in less than 30% of cases. Patients with osteosarcoma often develop resistance to chemotherapeutic agents, where personalized targeted therapies should offer new hope. T cell immunotherapy as a complementary or alternative treatment modality is advancing rapidly in general, but its potential against osteosarcoma remains largely unexplored. Strategies incorporating immune checkpoint inhibitors (ICIs), chimeric antigen receptor (CAR) modified T cells, and T cell engaging bispecific antibodies (BsAbs) are being explored to tackle relapsed or refractory osteosarcoma. However, osteosarcoma is an inherently heterogeneous tumor, both at the intra- and inter-tumor level, with no identical driver mutations. It has a pro-tumoral microenvironment, where bone cells, stromal cells, neovasculature, suppressive immune cells, and a mineralized extracellular matrix (ECM) combine to derail T cell infiltration and its anti-tumor function. To realize the potential of T cell immunotherapy in osteosarcoma, an integrated approach targeting this complex ecosystem needs smart planning and execution. Herein, we review the current status of T cell immunotherapies for osteosarcoma, summarize the challenges encountered, and explore combination strategies to overcome these hurdles, with the ultimate goal of curing osteosarcoma with less acute and long-term side effects.
Dihydroxyphenylacetaldehyde Lowering Treatment Improves Locomotor and Neurochemical Abnormalities in The Rat Rotenone Model: Relevance to The Catecholaldehyde Hypothesis for The Pathogenesis of Parkinson’s Disease
Authors: Khashab Rawan , Gutman-Sharabi Naama , Shabtai Zehava , Landau Regev , Halperin Reut , Fay-Karmon Tsviya , Leibowitz Avshalom , Sharabi Yehonatan
Published: August 2023

The catecholaldehyde hypothesis for the pathogenesis of Parkinson’s disease centers on accumulation of 3,4-dihydroxyphenylacetaldehyde (DOPAL) in dopaminergic neurons. To test the hypothesis, it is necessary to reduce DOPAL and assess if this improves locomotor abnormalities. Systemic administration of rotenone to rats reproduces the motor and central neurochemical abnormalities characterizing Parkinson’s disease. In this study, we used the monoamine oxidase inhibitor (MAOI) deprenyl to decrease DOPAL production, with or without the antioxidant N-acetylcysteine (NAC). Adult rats received subcutaneous vehicle, rotenone (2 mg/kg/day via a minipump), or rotenone with deprenyl (5 mg/kg/day i.p.) with or without oral NAC (1 mg/kg/day) for 28 days. Motor function tests included measures of open field activity and rearing. Striatal tissue was assayed for contents of dopamine, DOPAL, and other catechols. Compared to vehicle, rotenone reduced locomotor activity (distance, velocity and rearing); increased tissue DOPAL; and decreased dopamine concentrations and inhibited vesicular sequestration of cytoplasmic dopamine and enzymatic breakdown of cytoplasmic DOPAL by aldehyde dehydrogenase (ALDH), as indicated by DA/DOPAL and DOPAC/DOPAL ratios. The addition of deprenyl to rotenone improved all the locomotor indices, increased dopamine and decreased DOPAL contents, and corrected the rotenone-induced vesicular uptake and ALDH abnormalities. The beneficial effects were augmented when NAC was added to deprenyl. Rotenone evokes locomotor and striatal neurochemical abnormalities found in Parkinson’s disease, including DOPAL buildup. Administration of an MAOI attenuates these abnormalities, and NAC augments the beneficial effects. The results indicate a pathogenic role of DOPAL in the rotenone model and suggest that treatment with MAOI+NAC might be beneficial for Parkinson’s disease treatment.
Unlocking Sustainable Value through Digital Transformation: An Examination of ESG Performance
Authors: Kwilinski Aleksy , Lyulyov Oleksii , Pimonenko Tetyana
Published: August 2023

Digital transformation has already begun to play a significant role in helping EU countries to achieve sustainable values by promoting environmental, social and governance (ESG) efficiency. It is rapidly changing the economic landscape, which leads to changes in all sectors and at all levels. The European Union (EU) has set ambitious goals for sustainable development and climate change mitigation, such as the European Green Deal and the 2030 Agenda for Sustainable Development. The paper aims to test the spatial spillover effect of digitalization on ESG performance for EU countries for 2008–2020. The study applies the spatial Durbin model to check the research hypothesis. The empirical results revealed that the EU exhibits varying levels of ESG performance. Digital transformation has the potential to enhance ESG performance and has shown significant spatial spillover effects. The SDM estimates that a 1% increase in digital inclusion results in a minimal 0.001% increase in the ESG index. The statistically significant positive effects observed in key enablers, digital public services for businesses and citizens, highlight the contribution of digitalization to improving ESG performance. In addition, technological innovation serves as a critical conduit for transmitting digital transformation in the business and public sphere to ESG performance. Given these findings, policymakers are advised to strengthen digitalization efforts to narrow the digital divide, leveraging the digital economy as a potent instrument. Additionally, a dynamic and targeted strategy for digital economic development should be implemented to address ESG performance disparities effectively.
Implementation and Assessment of a Laparotomy-Assisted Three-Port Fetoscopic Spina Bifida Repair Program
Authors: Keil Corinna , Köhler Siegmund , Sass Benjamin , Schulze Maximilian , Kalmus Gerald , Belfort Michael , Schmitt Nicolas , Diehl Daniele , King Alice , Groß Stefanie , Sutton Caitlin D. , Joyeux Luc , Wege Mirjam , Nimsky Christopher , Whitehead Wiliam E. , Uhl Eberhard , Huisman Thierry A. G. M. , Neubauer Bernd A. , Weber Stefanie , Hummler Helmut , Axt-Fliedner Roland , Bedei Ivonne
Published: August 2023

Open spina bifida (OSB) is a congenital, non-lethal malformation with multifactorial etiology. Fetal therapy can be offered under certain conditions to parents after accurate prenatal diagnostic and interdisciplinary counseling. Since the advent of prenatal OSB surgery, various modifications of the original surgical techniques have evolved, including laparotomy-assisted fetoscopic repair. After a two-year preparation time, the team at the University of Giessen and Marburg (UKGM) became the first center to provide a three-port, three-layer fetoscopic repair of OSB via a laparotomy-assisted approach in the German-speaking area. We point out that under the guidance of experienced centers and by intensive multidisciplinary preparation and training, a previously described and applied technique could be transferred to a different setting.
The Risk and Predictors of Malignancies in Ankylosing Spondylitis Patients in Israel—A Retrospective Electronic Data-Based Study
Authors: Kagan Polina , Horesh Noy , Amital Howard , Tsur Avishai , Watad Abdulla , Cohen Arnon , Ben-Shabat Niv
Published: August 2023

Background: Previous studies demonstrated unclear and vast variability in the association between Ankylosing Spondylitis (AS) and the risk of cancer. Objectives: To assess the risk of overall and site-specific malignancies for AS patients in Israel, while examining the role of comorbidities and immunomodulatory therapy. Methods: We conducted a retrospective electronic data-based study including all AS patients diagnosed between 2002 and 2018, with no history of cancer prior to enrollment, with 5:1 ratio matched-control by age, gender, and place of residence. The odds Ratios (OR) for site-specific malignancies, comparing AS patients and controls, were calculated using logistic regression. Risk factors for malignancies within the AS cohort were evaluated in the same manner. Results: This study comprised 5825 AS patients and 28,356 matched controls. There was a higher overall risk of cancer in AS patients compared to controls (OR = 1.4, 95% CI 1.24–1.6), specifically for solid malignancies (OR = 1.5, 95% CI 1.3–1.7), CNS (OR = 3.72, 95% CI 1.29–10.7), kidney (OR = 2.06, 95% CI 1.12–3.8), and malignancy of unknown primary (OR = 3.06, 95% CI 2.35–3.98). Regarding predictors for malignancy within AS patients, older age at diagnosis (OR = 1.31, 95%,CI 1.25–2.36), diabetes (OR = 1.52, 95% CI 1.18–1.97), IBD (OR = 2.61, 95% CI 1.75–3.89), and treatment with DMARDs (OR = 2.17, 95% CI 1.65–2.83) were associated with a higher risk of solid malignancies, while NSAIDs treatment alone had a protective effect for solid malignancies (OR = 0.78, 95% CI 0.61–0.99). No significant association was found between anti-TNF therapy and the risk of solid or hematologic malignancies within the AS group. Conclusion: AS is associated with an increased risk of overall and site-specific malignancies, with independently higher risk for older age, comorbidity of DM, IBD, and treatment with DMARDs.
Three-Loop Technique for Pulley Reconstruction—A Retrospective Cohort Analysis of 23 Patients
Authors: Oeckenpöhler Simon , Langer Martin Franz , Aitzetmüller-Klietz Matthias Michael , Aitzetmüller-Klietz Marie-Luise , Nottberg Valerie , Riesenbeck Oliver
Published: August 2023

Twenty-three patients with a mean age of 52.7 years underwent pulley reconstruction using the Okutsu double- or triple-loop technique after iatrogenic or traumatic rupture of at least two adjacent flexor tendon pulleys in the finger and distal palm; mean age of injury was 4.77 years. The mean follow-up was 4.66 years after reconstruction of mostly A2 pulleys in a single surgeon setting. Outcome measures included ROM, NRS pain, satisfaction, Disabilities of Arm, Shoulder and Hand Questionnaire (DASH) and Krimmer score, Buck–Gramcko score, Jamar grip strength, pinch grip, and vigorimetry compared to the uninjured side. The median patient satisfaction score was 6.6/10. Hand function using the DASH score was 9.5. Grip strength on the Jamar Dynamometer showed only a slight reduction of 13% compared to the uninjured side. The resultant force of the operated fingers on the vigorimeter is almost 60% of that of the contralateral side, and the finger-palm distance of the operated finger was reduced from 2.2 cm to 1.45 cm. Other functional scores, such as Krimmer (82.2) and Buck-Gramcko (10.9), support these good results. The follow-up of patients more than 4.5 years after reconstruction of the A2 and A3 flexor tendon pulley using the double- or triple-loop technique showed acceptable patient satisfaction and good function of the finger in everyday life.
Correction: Pata et al. Sclerobanding (Combined Rubber Band Ligation with 3% Polidocanol Foam Sclerotherapy) for the Treatment of Second- and Third-Degree Hemorrhoidal Disease: Feasibility and Short-Term Outcomes. J. Clin. Med. 2022, 11, 218
Authors: Pata Francesco , Bracchitta Luigi Maria , D’Ambrosio Giancarlo , Bracchitta Salvatore
Published: August 2023

In the original publication [...]
Genetic Characteristics of Latvian Patients with Familial Hypercholesterolemia: The First Analysis from Genome-Wide Sequencing
Authors: Latkovskis Gustavs , Rescenko-Krums Raimonds , Nesterovics Georgijs , Briviba Monta , Saripo Vita , Gilis Dainus , Terauda Elizabete , Meiere Ruta , Skudrina Gunda , Erglis Andrejs , Chora Joana Rita , Bourbon Mafalda , Klovins Janis
Published: August 2023

Background: There is limited data on the genetic characteristics of patients with familial hypercholesterolemia (FH) in Latvia. We aim to describe monogenic variants in patients from the Latvian Registry of FH (LRFH). Methods: Whole genome sequencing with 30× coverage was performed in unrelated index cases from the LRFH and the Genome Database of Latvian Population. LDLR, APOB, PCSK9, LDLRAP1, ABCG5, ABCG8, LIPA, LPA, CYP27A1, and APOE genes were analyzed. Only variants annotated as pathogenic (P) or likely pathogenic (LP) using the FH Variant Curation Expert Panel guidelines for LDLR and adaptations for APOB and PCSK9 were reported. Results: Among 163 patients, the mean highest documented LDL-cholesterol level was 7.47 ± 1.60 mmol/L, and 79.1% of patients had LDL-cholesterol ≥6.50 mmol/L. A total of 15 P/LP variants were found in 34 patients (diagnostic yield: 20.9%): 14 in the LDLR gene and 1 in the APOB gene. Additionally, 24, 54, and 13 VUS were detected in LDLR, APOB, and PCSK9, respectively. No P/LP variants were identified in the other tested genes. Conclusions: Despite the high clinical likelihood of FH, confirmed P/LP variants were detected in only 20.9% of patients in the Latvian cohort when assessed with genome-wide next generation sequencing.
Investigating Planning and Non-Targeted Exploration in PIAAC 2012: Validating Their Measures Based on Process Data and Investigating Their Relationships with Problem-Solving Competency
Authors: Zhang Maoxin , Andersson Björn , Greiff Samuel
Published: August 2023

Problem-solving is a critical aspect of intelligence that has become increasingly important in modern society. Mapping out the determinants of success in problem-solving helps understand the underlying cognitive processes involved. This article focuses on two key cognitive processes in problem-solving: non-targeted exploration and planning. We generalize previously defined indicators of planning and non-targeted exploration across tasks in the 2012 Programme for the International Assessment of Adult Competencies and examine the internal construct validity of the indicators using confirmatory factor analysis. We also investigate the relationships between problem-solving competency, planning, and non-targeted exploration, along with the specific dependence between indicators from the same task. The results suggest that (a) the planning indicator across tasks provides evidence of internal construct validity; (b) the non-targeted exploration indicator provides weaker evidence of internal construct validity; (c) overall, non-targeted exploration is strongly related to problem-solving competency, whereas planning and problem-solving competencies are weakly negatively related; and (d) such relationships vary substantially across tasks, emphasizing the importance of accounting for the dependency of measures from the same task. Our findings deepen our understanding of problem-solving processes and can support the use of digital tools in educational practice and validate task design by comparing the task-specific relationships with the desired design.
Does Using None-of-the-Above (NOTA) Hurt Students’ Confidence?
Authors: Little Jeri L.
Published: August 2023

Students claim that multiple-choice questions can be tricky, particularly those with competitive incorrect choices or choices like none-of-the-above (NOTA). Additionally, assessment researchers suggest that using NOTA is problematic for assessment. In experiments conducted online (with trivia questions) and in the classroom (with course-related questions), I investigated the effects of including NOTA as a multiple-choice choice alternative on students’ confidence and performance. In four experiments, participants answered two types of questions: basic multiple-choice questions (basic condition) and equivalent questions in which one incorrect choice was replaced with NOTA (NOTA condition). Immediately after answering each question, participants rated their confidence in their answer to that question (item-by-item confidence). At the end of the experiments, participants made aggregate confidence judgments for the two types of questions and provided additional comments about the use of NOTA as an alternative. Surprisingly, I found no significant differences in item-by-item confidence or performance between the two conditions in any of the experiments. However, across all four experiments, when making aggregate judgments, participants provided lower confidence estimates in the NOTA condition than in the basic condition. Although people often report that NOTA questions hurt their confidence, the present results suggest that they might not—at least not on a question-by-question basis.
Investigation of Submergence Depth and Wave-Induced Effects on the Performance of a Fully Passive Energy Harvesting Flapping Foil Operating Beneath the Free Surface
Authors: Petikidis Nikos , Papadakis George
Published: August 2023

This paper investigates the performance of a fully passive flapping foil device for energy harvesting in a free surface flow. The study uses numerical simulations to examine the effects of varying submergence depths and the impact of monochromatic waves on the foil’s performance. For the numerical simulations, a in-house artificial compressibility two-phase solver is employed and coupled with a rigid body dynamic solver. The results show that the fully passive flapping foil device can achieve high efficiency for submergence depths between 4 and 9 chords, with an “optimum” submergence depth where the flapping foil performance is maximised. The effects of regular waves on the foil’s performance were also investigated, showing that waves with a frequency close to that of the natural frequency of the flapping foil-aided energy harvesting. Overall, this study provides insights that could be useful for future design improvements for fully passive flapping foil devices for energy harvesting operating near the free surface.
The Need for an Environmental Notification System in the Lithuanian Coastal Area
Authors: Šakurova Ilona , Kondrat Vitalijus , Baltranaitė Eglė , Gardauskė Vita
Published: August 2023

The Lithuanian coastal area is divided by the jetties of the Port of Klaipėda and represents two geomorphologically distinct parts. Local companies and institutions contribute to shaping the coastal area through infrastructure development. Awareness of the changes in the coastal zone can play an important role in the planning and economic feasibility of activities in the Klaipėda coastal region. Therefore, developing a notification system that provides long– and short–term monitoring data for the Lithuanian coastal zone is necessary. In order to do so, the authors intend to create a system that should provide a link between long– and short–term observation and monitoring data for stakeholders, such as wind speed and direction, wave direction and significant height, water and air temperature, atmospheric pressure, sediment size, and distribution, height above sea level, shoreline position, beach width, change in beach protection measures, beach wreckage, and marine debris management, in order to provide timely notifications to end users.
Coastal Engineering: Sustainability and New Technologies
Authors: Esteban M. Dolores , López-Gutiérrez José-Santos , Negro Vicente , Neves M. Graça
Published: August 2023

Coastal engineering is a constantly evolving discipline, in which it is essential to seek a balance between the natural character of the coastal zone and the integration, to a greater or lesser extent, of human activities in that space [...]
The Effect of Oleic Acid-Enriched Diet in Hybrid Groupers (Epinephelus fuscoguttatus × Epinephelus lanceolatus) upon Infection with Vibrio vulnificus Using an LC-qTOF-MS Approach
Authors: Natnan Maya Erna , Low Chen-Fei , Chong Chou-Min , Rungrassamee Wanilada , Baharum Syarul Nataqain
Published: August 2023

The aim of this study was to evaluate the impact of oleic acid supplements on the liver metabolome of hybrid grouper fingerlings (Epinephelus fuscoguttatus × Epinephelus lanceolatus) challenged with Vibrio vulnificus. Oleic acid was used as a fish feed supplement because it has been reported to enhance the immune response of fish. After six weeks of feeding trials with the control and experimental diets (oleic acid immunostimulant), 10 fish were randomly selected from each treatment and challenged with V. vulnificus by immersion for 30 min. After seven days of post-bacterial challenge, the liver samples of surviving infected groupers were dissected and used for metabolomics fingerprinting using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-qTOF-MS). The results were then analyzed using MetaboAnalyst 5.0 and SIMCA+P software. From the analyses conducted, out of 639 primary metabolites detected, a total of 66 metabolites were significantly identified from the liver samples of grouper fed control and oleic acid diets. Further analysis also showed that the control and oleic acid diets have significantly different metabolites by the discriminating clustering of samples based on the PLS-DA analysis. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, the known metabolites pathways with high impact values are alanine, aspartate, and glutamate metabolism (0.13) followed by purine metabolism pathways (0.05). Moreover, out of these highly impacted metabolic pathways, L-glutamine (8.71%), L-lysine (4.05%), L-carnitine (11.53%), and inosine (10.49%) were the several metabolites that were highly abundant in the liver sample of surviving infected hybrid groupers fed with dietary oleic acid. The changes of metabolites contributed to improving the immune system of the fish. Thus, our results advance our understanding of the immunological regulation of the hybrid groupers’ immune response toward oleic acid immunostimulants against Vibrio infection.
Human and Feline Sporotrichosis in a Reference Center of Southeastern Brazil: Genetic Differentiation, Diversity, and Antifungal Susceptibility of Sporothrix Species
Authors: Gonçalves Sarah Santos , da Cruz Bahiense Rocha Isabela , Rediguieri Bruno Carneiro , de Carvalho Jamile Ambrósio , Maifrede Simone Bravim , Kruschewsky Wdson Luis Lima , Falqueto Aloísio , Rodrigues Anderson Messias
Published: August 2023

Sporotrichosis is a neglected subcutaneous fungal infection that affects humans and animals worldwide caused by species belonging to the genus Sporothrix. This study aims to examine the range of genetic variations, assess molecular epidemiology significance, and explore potential modes of transmission of the Sporothrix species associated with the current sporotrichosis outbreaks in Espírito Santo, Brazil. In this investigation, 262 samples were evaluated, including 142 from humans and 120 from felines, collected between 2016 and 2021. The isolates were identified based on morphological and molecular characteristics. Sexual idiomorphs were determined by mating-type PCR using primers specific to the MAT1-1 and MAT1-2 loci. Amplified fragment length polymorphism (AFLP) was employed to assess the genetic variability of Sporothrix spp. Finally, antifungal susceptibility testing was performed following the CLSI M38-A2 protocol. Of the 142 human samples, 125 were identified as S. brasiliensis and 17 as S. schenckii s. str. The presence of S. brasiliensis was overwhelming (100%) during outbreaks, highlighting the significant role of domestic cats in the emergence of this species. Heterothallism was the only observed mating strategy. However, the MAT1-2 idiomorph was predominant in cases of cat-transmitted sporotrichosis (χ2 = 202.976; p < 0.0001). Our AFLP results show significant intraspecific variability observed among S. brasiliensis isolates in Espírito Santo. Different genotypes forming subgroups within the same population suggest that these isolates do not originate from a single ancestor, indicating multiple emergences. Furthermore, terbinafine was the antifungal with the best results in vitro. However, in clinical practice, itraconazole remains the primary treatment choice. Sporotrichosis continues to advance in the state; therefore, the health system must outline one-health strategies to contain the disease to prevent future epidemics.
Spatial Agglomeration and Coupling Coordination of Population, Economics, and Construction Land in Chinese Prefecture-Level Cities from 2010 to 2020
Authors: Cai Enxiang , Zhao Xinyu , Zhang Shengnan , Li Ling
Published: August 2023

Exploring the spatial pattern and development strategies of urbanization from the perspective of the multi-dimensional coordination of population, economy, and land is the key to solving the problems of the urban–rural gap and human–land contradiction. This paper analyzed the spatial agglomeration of population, economy, and construction land area growth rates and explored their coordinated development in Chinese prefecture-level cities from 2010 to 2020 by using the spatial autocorrelation model, elasticity coefficient model, and coupling coordination model. The results are as follows: (1) China’s population, economy, and construction land area were all growing, with the highest economic growth and the lowest population growth, and most prefecture-level cities in central and northeastern China had negative population growth. (2) The growth rates of the population, economy, and construction land in Chinese prefecture-level cities had significant positive spatial clustering characteristics; the spatial agglomeration of the economy was the most prominent and the high-value areas were mainly concentrated in western China. (3) The elasticity coefficients between the population, economy, and construction land in most Chinese prefecture-level cities indicate uneven development of urbanization, manifested as population growth lagging behind construction land expansion and further lagging behind economic development. (4) More than 56% of Chinese prefecture-level cities have uncoordinated development among the population, economy, and construction land mainly distributed in northeast China and central China. The results can provide references and decision-making support for promoting the sustainable development of China’s new urbanization.
Response of Urban Ecosystem Carbon Storage to Land Use/Cover Change and Its Vulnerability Based on Major Function-Oriented Zone Planning
Authors: Geng Lili , Zhang Yuanyuan , Hui Huixian , Wang Yuhan , Xue Yongji
Published: August 2023

Vigorous emphasis has been placed on optimizing land spatial planning to protect carbon storage and enhance ecosystem resilience. What is the effectiveness of the Major Function-Oriented Zone (MFOZ) planning implemented to achieve this goal in China? Especially in urbanized areas where there are more pronounced conflicts between humans and land. Taking the Beijing-Tianjin-Hebei (BTH) urban agglomeration as the target area, this study explored the response of carbon storage to land use/cover change (LUCC) and its vulnerability to ecological service functions under MFOZ planning. The 30 m × 30 m spatially resolved Landsat TM/ETM remote sensing images from 2000 to 2020 were used. The data preprocessing was performed mainly through radiometric calibration, clipping, and reclassification through the ArcGIS 10.7 software. Applying the InVEST model, which uses the LUCC map and carbon storage density of the four carbon pools, including above-ground carbon density, below-ground carbon density, dead organic carbon density, and soil organic carbon density, to evaluate the carbon storage under the current landscape or in the future, the results show that: (1) The BTH ecosystem experienced a carbon storage reduction of about 7.25 × 107 Mg from 2000 to 2020 due to the expansion of construction land, which crowded out cropland. Carbon storage in the BTH showed a high concentration in the “northeast-southwest” direction and a tiny distribution in the “middle-east” direction. (2) From 2015, the initial effects of the MFOZ planning were seen, with the ecological land in the Central Core Zone and Eastern Coastal Development Zone decreasing while the proportion of high-carbon storage areas in the Eastern Coastal Development Zone increasing. (3) Over the two decades, the land use intensity index improved by 4.65 overall, and vulnerability worsened from 2000 to 2015 and was alleviated from 2015 to 2020. This study will provide a scientific reference for optimizing urban spatial land use planning and promoting carbon sequestration in ecosystems.