bluuebunny/crossref_metadata_2025 huggingface dataset:
Significant Reversal of Facial Wrinkle, Pigmented Spot and Roughness by Daily Application of Galactomyces Ferment Filtrate-Containing Skin Products for 12 Months—An 11-Year Longitudinal Skin Aging Rejuvenation Study
Authors: Miyamoto Kukizo , Inoue Yasuko , Yan Xianghong , Yagi Shiomi , Suda Sudarsana , Furue Masutaka
Published: February 2023

Facial skin aging is an important psychophysical and social concern, especially in women. We compared facial parameters reflecting aging of the skin in 1999 and 2010 in 86 female volunteers. Then, all subjects applied three Galactomyces ferment filtrate-containing skin care products (G3 products; SK-II Facial Treatment Essence, SK-II Cellumination Essence, and SK-II Skin Signature Cream) twice daily for 12 months (M), with the skin parameters being measured at 2 M, 8 M, and 12 M during this period. Facial skin aging parameters such as wrinkles, hyperpigmented spots, and roughness significantly deteriorated during the 11-year interval. This 11-year aging process was associated with reduced hydration and increased transepidermal water loss (TEWL). Notably, treatment with G3 products significantly and cumulatively increased skin hydration with a correlated reduction of TEWL during the 12 M treatment period. Such treatment also significantly and cumulatively reversed the 11-year facial skin aging in the three parameters of wrinkles, spots, and roughness. These results suggest that facial skin retains the potential to recover from the aging process when it is applied with appropriate cosmetic agents.
Tinnitus and Its Relation to Depression, Anxiety, and Stress—A Population-Based Cohort Study
Authors: Hackenberg Berit , Döge Julia , O’Brien Karoline , Bohnert Andrea , Lackner Karl J. , Beutel Manfred E. , Michal Matthias , Münzel Thomas , Wild Philipp S. , Pfeiffer Norbert , Schulz Andreas , Schmidtmann Irene , Matthias Christoph , Bahr Katharina
Published: February 2023

Tinnitus is a common symptom reported in otolaryngologic practice. Although the pathophysiology of tinnitus has not been fully understood, clinical studies suggest that psychological symptoms of depression, anxiety, and somatization are increased in tinnitus patients. However, patients seeking medical treatment for tinnitus may be especially vulnerable. Population-based studies reporting on the association between tinnitus and psychological distress are still lacking. The aim of this study was to investigate the correlation of tinnitus with depression, anxiety, or somatization in a large population-based cohort. The Gutenberg Health Study is a population-based cohort study. Participants were asked about the occurrence of tinnitus (yes/no) and how much they were bothered by it. In addition, they completed the PHQ-9, GAD-7, and SSS-8 questionnaires to assess depressive symptoms, anxiety, and somatic symptom disorders. A total of 8539 participants were included in the study cohort. Tinnitus prevalence was 28.0% (2387). The prevalence of depression/anxiety/somatic symptom disorders was significantly higher among participants with tinnitus than among participants without tinnitus (7.9%/5.4%/40.4% participants with tinnitus vs. 4.6%/3.3%/26.9% participants without tinnitus, p-value < 0.0001). Logistic regression results showed that participants with tinnitus were more likely to suffer from depression (OR = 2.033, 95% CI [1.584; 2.601], p-value < 0.0001), anxiety (OR = 1.841, 95% CI [1.228; 2.728], p-value = 0.0027), or somatic symptom disorders (OR = 2.057, 95% CI [1.799; 2.352], p-value < 0.0001). Symptoms of depression, anxiety, and somatic symptom disorders were increased in participants with tinnitus. This must be taken into account when treating these patients.
Safety and Efficacy of Surgery for Metastatic Tumor to the Pancreas: A Single-Center Experience
Authors: Moletta Lucia , Friziero Alberto , Serafini Simone , Grillo Valeria , Pierobon Elisa Sefora , Capovilla Giovanni , Valmasoni Michele , Sperti Cosimo
Published: February 2023

Pancreatic metastases from other neoplasms are rare. The role of surgery for this clinical entity is unclear. The aim of this study was to investigate the role of resection in patients with pancreatic secondary lesions. We observed 44 patients with pancreatic metastases from other tumors. Renal cell carcinoma was the most common primary tumor (n = 19, 43.2%). Thirty-seven patients underwent surgery, and pancreatic resection with curative intent was feasible in 35 cases. Fifteen patients (43.2%) experienced major postoperative complications (Clavien-Dindo > 2), and postoperative mortality rate was 5.4%. The median overall survival and disease-free survival were 38 (range 0–186) and 11 (range 0–186) months, respectively. Overall survival and disease-free survival were significantly longer for pancreatic metastases from renal cell carcinoma when compared to other primary tumors. Multivariate analysis confirmed a pathological diagnosis of metastasis from RCC as an independent prognostic factor for overall survival (OR 2.48; 95% CI, 1.00–6.14; p = 0.05). In conclusion, radical resection of metastases to the pancreas is feasible and safe, and may confer a survival benefit for selected patients. There is a clear benefit of metastasectomy in terms of patient survival for metastases from renal cell carcinoma, while for those with other primary tumors, surgery seems to be mainly palliative.
Influence of Guanine-Based Purines on the Oxidoreductive Reactions Involved in Normal or Altered Brain Functions
Authors: Zuccarini Mariachiara , Pruccoli Letizia , Balducci Martina , Giuliani Patricia , Caciagli Francesco , Ciccarelli Renata , Di Iorio Patrizia
Published: February 2023

The production of reactive oxygen species (ROS) in the brain is homeostatically controlled and contributes to normal neural functions. Inefficiency of control mechanisms in brain aging or pathological conditions leads to ROS overproduction with oxidative neural cell damage and degeneration. Among the compounds showing therapeutic potential against neuro-dysfunctions induced by oxidative stress are the guanine-based purines (GBPs), of which the most characterized are the nucleoside guanosine (GUO) and the nucleobase guanine (GUA), which act differently. Indeed, the administration of GUO to in vitro or in vivo models of acute brain injury (ischemia/hypoxia or trauma) or chronic neurological/neurodegenerative disorders, exerts neuroprotective and anti-inflammatory effects, decreasing the production of reactive radicals and improving mitochondrial function via multiple molecular signals. However, GUO administration to rodents also causes an amnesic effect. In contrast, the metabolite, GUA, could be effective in memory-related disorders by transiently increasing ROS production and stimulating the nitric oxide/soluble guanylate cyclase/cGMP/protein kinase G cascade, which has long been recognized as beneficial for cognitive function. Thus, it is worth pursuing further studies to ascertain the therapeutic role of GUO and GUA and to evaluate the pathological brain conditions in which these compounds could be more usefully used.
Serum KL-6 as a Biomarker of Progression at Any Time in Fibrotic Interstitial Lung Disease
Authors: Jehn Lutz B. , Costabel Ulrich , Boerner Eda , Wälscher Julia , Theegarten Dirk , Taube Christian , Bonella Francesco
Published: February 2023

The development of a progressive phenotype of interstitial lung disease (ILD) is still unpredictable. Whereas tools to predict mortality in ILD exist, scores to predict disease progression are missing. The aim of this study was to investigate whether baseline serum KL-6 as an established marker to assess disease activity in ILD, alone or in combination with clinical variables, could improve stratification of ILD patients according to progression risk at any time. Consecutive patients with fibrotic ILD, followed at our institution between 2008 and 2015, were investigated. Disease progression was defined as relative decline of ≥10% in forced vital capacity (FVC) or ≥15% in diffusing capacity of the lung for carbon monoxide (DLco)% from baseline at any time. Serum KL-6 was measured using an automated immunoassay (Fujirebio Europe, Gent, Belgium). A stepwise logistic regression was performed to select variables to be included in the score. A total of 205 patients (49% idiopathic pulmonary fibrosis (IPF), 51% fibrotic nonspecific interstitial pneumonia (NSIP)) were included, of them 113 (55%) developed disease progression during follow up. Male gender (G) and serum KL-6 strata (K) were significant predictors of progression at regression analysis and were included in the GK score. A threshold of 2 GK score points was best for discriminating patients at high risk versus low risk to develop disease progression at any time. Serum KL-6 concentration, alone or combined in a simple score with gender, allows an effective stratification of ILD patients for risk of disease progression at any time.
Efficacy and Treatment Satisfaction of Different Systemic Therapies in Children and Adolescents with Moderate-to-Severe Atopic Dermatitis: A Real-World Study
Authors: Kiefer Sebastian , König Anke , Gerger Viviane , Rummenigge Christine , Müller Anne Christine , Jung Thomas , Frank Alexandra , Tassopoulos Georgios , Laurent Emilie , Kaufmann Roland , Pinter Andreas
Published: February 2023

For the treatment of moderate-to-severe atopic dermatitis in children and adolescents, the monoclonal antibody dupilumab and the selective JAK-1 inhibitor upadacitinib are two modern systemic therapies approved for long-term treatment. Both drugs have demonstrated high efficacy in randomized controlled trials, although evidence from real-world data in the pediatric population is limited. In a prospective analysis over 24 weeks, we investigated the efficacy, safety and treatment satisfaction of both systemic therapies in 23 patients (16 patients treated with dupilumab; 7 patients treated with upadacitinib). The median age of the patients was 16 years, with a median EASI of 18.8. A significant improvement in the EASI, VAS-itch, CDLQI, POEM and DFIQ from baseline to week 24 was demonstrated for both treatment options. No significant difference was observed between dupilumab and upadacitinib in any of the assessed scores. Less adverse events were recorded in the real-world setting compared with clinical trials. Our results confirm the efficacy and safety of dupilumab and upadacitinib as equivalent treatment options in children and adolescents in a real-world setting.
Predict the Effects of Dolutegravir (DTG) Plus Lamivudine (3TC) on Immunological Responses in People Living with HIV (PLWHIV)
Authors: Troya Jesús , Pedrero-Tomé Roberto , Buzón Luis , Dueñas Carlos ,   on behalf of the SPADE Study Group
Published: February 2023

Background: Immune recovery in people living with HIV (PLWHIV) is a residual aspect of antiretroviral treatment (ART) in most patients, but in a non-negligible proportion of them, the CD4+ lymphocytes count, or CD4/CD8 ratio remains suboptimal. Methods: We performed a model of the immune response after 24 weeks of switching to a 2DR with DTG plus 3TC in a retrospective multicenter cohort of undetectable and experienced patients using significant predictor variables associated with the parameters or situations defined as success and failure. Clinical variables studied were CD4+ and CD8+ lymphocyte count, percentage of CD4, and CD4/CD8 ratio. These parameters were assessed at baseline and 24 weeks after the switch. Based on the evolution of each variable, four categories of immune response and four categories of non-immune response were defined. Immune response was defined as CD4+ count > 500 cells/mm3, %CD4 > 30%, CD8+ count < 1000 cells/mm3 and CD4/CD8 ratio ≥ 0.9. Non-response is just the opposite. Results: In our different models of immunological response, the presence of stage of AIDS (p = 0.035, p = 0.065) and current age over 50 years (p = 0.045) are postulated as statistically significative limiting factors in achieving an improvement in CD4, %CD4, CD8, and CD4/CD8 ratio. Late HIV diagnosis (p = 0.156), without statistical significance, enhanced late the previous variables. In contrast, conditions where patients start with CD4 > 500 cells/mm3 (p = 0.054); CD4 > 30% (p = 0.054, p = 0.084); CD8 < 1000 cells/mm3 (p = 0.018), and CD4/CD8 ≥ 0.9 (p = 0.013, p = 0.09) are detected as stimulating or conducive to DTG plus 3TC treatment success. Conclusion: These models represent a proof of concept that could become a valuable tool for clinicians to predict the effects of DTG plus 3TC on immunological responses prior to the switch in undetectable pre-treated PLWHIV with immune dysfunction. The main predictors for immunological failure were late HIV diagnosis, stage of AIDS, and current age over 50 years. In contrast, starting with a normalized immune status was detected as stimulating or conducive to DTG plus 3TC treatment success.
miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL
Authors: Zhang Jingyong , Wang Shubao , Bai Yang , Ali Aasi Mohammad , Deng Jiewen , Chen Yushi , Fu Yonghui , He Ming
Published: February 2023

First-line treatment for osteosarcoma includes chemotherapy and surgery. However, the five-year survival rate of refractory osteosarcoma remains unsatisfactory. Osteosarcoma cancer stem cells, possessing stemness and chemoresistance, are one of the critical causes of poor response to chemotherapy. Elucidating regulatory signaling pathways of osteosarcoma cancer stem cells may provide a rationale for improving regimens against chemoresistant osteosarcoma. Methotrexate (MTX)-resistant osteosarcoma cells were established. microRNA expression profiles were used for detecting differentially expressed microRNA in resistant clones and the parental cells. microRNA target databases were employed to predict potential microRNA and mRNA interactions. Flow cytometry was performed to measure stem cell marker Prominin-1 (CD133)-positive cells. Immunofluorescence staining was applied to detect CD133 expression. miR-197-3p mimic or anti-miR-197-3p stably transfected cells were used to generate xenograft models. In the study, we found that miR-197-3p was increased in MTX-resistant cell lines. Overexpression of miR-197-3p enhanced the expression of cancer stem cell markers CD133, Octamer-binding protein 4 (OCT4), Transcription factor SOX-2 (SOX2), and Homeobox protein NANOG (NANOG), as well as chemoresistance-associated genes ATP-dependent translocase ABCB1 (ABCB1) and Broad substrate specificity ATP-binding cassette transporter ABCG2 (ABCG2), whereas miR-197-3p knockdown inhibited stemness and recovered sensitivity to MTX. We also classified the tumor suppressor Speckle-type POZ protein-like (SPOPL) as a target of miR-197-3p. The miR-197-3p mutation that could not combine SPOPL promoter regions was unable to sustain stemness or chemoresistance. Collectively, we discovered miR-197-3p conferred osteosarcoma stemness and chemotherapy resistance by targeting SPOPL, prompting promising therapeutic candidates for refractory osteosarcoma treatment.
Prospective Assessment of Cerebral Microbleeds with Low-Field Magnetic Resonance Imaging (0.55 Tesla MRI)
Authors: Rusche Thilo , Breit Hanns-Christian , Bach Michael , Wasserthal Jakob , Gehweiler Julian , Manneck Sebastian , Lieb Johanna M. , De Marchis Gian Marco , Psychogios Marios , Sporns Peter B.
Published: February 2023

Purpose: Accurate detection of cerebral microbleeds (CMBs) on susceptibility-weighted (SWI) magnetic resonance imaging (MRI) is crucial for the characterization of many neurological diseases. Low-field MRI offers greater access at lower costs and lower infrastructural requirements, but also reduced susceptibility artifacts. We therefore evaluated the diagnostic performance for the detection of CMBs of a whole-body low-field MRI in a prospective cohort of suspected stroke patients compared to an established 1.5 T MRI. Methods: A prospective scanner comparison was performed including 27 patients, of whom 3 patients were excluded because the time interval was >1 h between acquisition of the 1.5 T and 0.55 T MRI. All SWI sequences were assessed for the presence, number, and localization of CMBs by two neuroradiologists and additionally underwent a Likert rating with respect to image impression, resolution, noise, contrast, and diagnostic quality. Results: A total of 24 patients with a mean age of 74 years were included (11 female). Both readers detected the same number and localization of microbleeds in all 24 datasets (sensitivity and specificity 100%; interreader reliability ϰ = 1), with CMBs only being observed in 12 patients. Likert ratings of the sequences at both field strengths regarding overall image quality and diagnostic quality did not reveal significant differences between the 0.55 T and 1.5 T sequences (p = 0.942; p = 0.672). For resolution and contrast, the 0.55 T sequences were even significantly superior (p < 0.0001; p < 0.0003), whereas the 1.5 T sequences were significantly superior (p < 0.0001) regarding noise. Conclusion: Low-field MRI at 0.55 T may have similar accuracy as 1.5 T scanners for the detection of microbleeds and thus may have great potential as a resource-efficient alternative in the near future.
Clinical Predictors and Prognosis of Myocardial Infarction with Non-Obstructive Coronary Arteries (MINOCA) without ST-Segment Elevation in Older Adults
Authors: Gabaldon-Perez Ana , Bonanad Clara , Garcia-Blas Sergio , Marcos-Garcés Víctor , D’Gregorio Jessika Gonzalez , Fernandez-Cisnal Agustín , Valero Ernesto , Minana Gema , Merenciano-González Héctor , Mollar Anna , Bodi Vicente , Nunez Julio , Sanchis Juan
Published: February 2023

A non-neglectable percentage of patients with non-ST elevation myocardial infarction (NSTEMI) show non-obstructive coronary arteries (MINOCA). Specific data in older patients are scarce. We aimed to identify the clinical predictors of MINOCA in older patients admitted for NSTEMI and to explore the long-term prognosis of MINOCA. This was a single-center, observational, consecutive cohort study of older (≥70 years) patients admitted for NSTEMI between 2010 and 2014 who underwent coronary angiography. Univariate and multivariate Cox regression were performed to analyze the association of variables with MINOCA and all-cause mortality and with major adverse cardiac events (MACE), defined as a combined endpoint of all-cause mortality and nonfatal myocardial infarction and a combined endpoint of cardiovascular mortality, nonfatal myocardial infarction, and unplanned revascularization. The registry included 324 patients (mean age 78.8 ± 5.4 years), of which 71 (21.9%) were diagnosed with MINOCA. Predictors of MINOCA were female sex, left bundle branch block, pacemaker rhythm, chest pain at rest, peak troponin level, previous MI, Killip ≥2, and ST segment depression. Regarding prognosis, patients with obstructive coronary arteries (stenosis ≥50%) and the subgroup of MINOCA patients with plaques <50% had a similar prognosis; while MINOCA patients with angiographically smooth coronary arteries had a reduced risk of MACE. We conclude that the following: (1) in elderly patients admitted for NSTEMI, certain universally available clinical, electrocardiographic, and analytical variables are associated with the diagnosis of MINOCA; (2) elderly patients with MINOCA have a better prognosis than those with obstructive coronary arteries; however, only those with angiographically smooth coronary arteries have a reduced risk of all-cause mortality and MACE.
Impacts of COVID-19 Pandemic on Sleep Quality Evaluated by Wrist Actigraphy: A Systematic Review
Authors: Ferreira-Souza Luiz Felipe , Julianelli-Peçanha Marize , Coelho-Oliveira Ana Carolina , da Silva Bahia Christianne Martins Corrêa , Paineiras-Domingos Laisa Liane , Reis-Silva Aline , Moura-Fernandes Márcia Cristina , Trindade-Gusmão Luiza Carla , Taiar Redha , da Cunha Sá-Caputo Danubia , Rapin Amandine , Bernardo-Filho Mario
Published: February 2023

COVID-19 has probably contributed as a risk factor for sleep disturbance. Actigraphy has been used to evaluate sleep complaints in self-isolated populations and frontline doctors during the COVID-19 pandemic. This systematic review aims to summarize the impact of the COVID-19 pandemic on sleep through wrist actigraphy, estimating sleep latency, total sleep time, awakening-after-sleep onset, and sleep efficiency. Searches were conducted of observational studies on the PubMed, Embase, Scopus, Web of Science, and PEDro databases from 1 December 2019 to 31 December 2022. Ninety articles were found, and given the eligibility criteria, fifteen were selected. Six studies were classified by the National Health and Medical Research Council as evidence level IV, two studies as level III-3, and seven studies as level III-2. According to the ACROBAT-NRSI instrument, three studies were classified as having a “serious” risk of bias, two as having “critical” risk, four as having “moderate” risk, and six as having “low” risk. In the selected publications, various populations were evaluated via actigraphy during the COVID-19 pandemic, with reports of “poor” sleep quality. Actigraphy may be a relevant tool to assess individual day–night rhythms and provide recommendations under enduring pandemic conditions. Moreover, as actigraphy presents objective data for sleep evaluations, it is suggested that this method be used in similar pandemics and that actigraphy be included as part of the sleep hygiene strategy.
Stereotactic MR-Guided Radiotherapy for Liver Metastases: First Results of the Montpellier Prospective Registry Study
Authors: Bordeau Karl , Michalet Morgan , Keskes Aïcha , Valdenaire Simon , Debuire Pierre , Cantaloube Marie , Cabaillé Morgane , Jacot William , Draghici Roxana , Demontoy Sylvain , Quantin Xavier , Ychou Marc , Assenat Eric , Mazard Thibault , Gauthier Ludovic , Dupuy Marie , Guiu Boris , Bourgier Céline , Aillères Norbert , Fenoglietto Pascal , Azria David , Riou Olivier
Published: February 2023

Liver stereotactic body radiotherapy (SBRT) is a local treatment that provides good local control and low toxicity. We present the first clinical results from our prospective registry of stereotactic MR-guided radiotherapy (MRgRT) for liver metastases. All patients treated for liver metastases were included in this prospective registry study. Stereotactic MRgRT indication was confirmed by multidisciplinary specialized tumor boards. The primary endpoints were acute and late toxicities. The secondary endpoints were survival outcomes (local control, overall survival (OS), disease-free survival, intrahepatic relapse-free survival). Twenty-six consecutive patients were treated for thirty-one liver metastases between October 2019 and April 2022. The median prescribed dose was 50 Gy (40–60) in 5 fractions. No severe acute MRgRT-related toxicity was noted. Acute and late gastrointestinal and liver toxicities were low and mostly unrelated to MRgRT. Only 5 lesions (16.1%) required daily adaptation because of the proximity of organs at risk (OAR). With a median follow-up time of 17.3 months since MRgRT completion, the median OS, 1-year OS and 2-year OS rates were 21.7 months, 83.1% (95% CI: 55.3–94.4%) and 41.6% (95% CI: 13.5–68.1%), respectively, from MRgRT completion. The local control at 6 months, 1 year and 2 years was 90.9% (95% CI: 68.3–97.7%). To our knowledge, we report the largest series of stereotactic MRgRT for liver metastases. The treatment was well-tolerated and achieved a high LC rate. Distant relapse remains a challenge in this population.
A Review of Modern Imaging Landscape for Prostate Cancer: A Comprehensive Clinical Guide
Authors: Gravestock Paul , Somani Bhaskar Kumar , Tokas Theodoros , Rai Bhavan Prasad
Published: February 2023

The development of prostate cancer imaging is rapidly evolving, with many changes to the way patients are diagnosed, staged, and monitored for recurrence following treatment. New developments, including the potential role of imaging in screening and the combined diagnostic and therapeutic applications in the field of theranostics, are underway. In this paper, we aim to outline the current landscape in prostate cancer imaging and look to the future at the potential modalities and applications to come.
Trends in Gynecologic Carcinosarcoma Based on Analysis of the Surveillance Epidemiology End Result (SEER) Database
Authors: Lee Joo Won , Ouh Yung-Taek , Chang Ha Kyun , Min Kyung-Jin , Lee Sanghoon , Hong Jin-Hwa , Song Jae-Yun , Lee Jae-Kwan , Lee Nak Woo
Published: February 2023

Carcinosarcomas (malignant mixed Mullerian tumors) of a female genital organ are rare tumors associated with a poor survival. The purpose of this study was to identify site-specific differences in the incidence and prognosis in carcinosarcomas originating in the uterus, cervix, or ovary. The data of patients with gynecologic carcinosarcomas were extracted from the Surveillance, Epidemiology, and End Results (SEER) database between 2000 and 2016. The characteristics of gynecologic carcinosarcomas were compared using Pearson X2 and Fisher’s exact tests. Kaplan–Meier models were used for cause-specific survival (CSS) analysis. The cohort included 7086 females, including 5731 cases of uterine carcinosarcoma, 161 cervical carcinosarcomas, and 1193 ovarian carcinosarcomas. The age-adjusted incidence rates of uterine, cervical, and ovarian carcinosarcoma were 3.9, 0.1, and 0.6 per 1,000,000, respectively. In the distribution of carcinosarcoma incidence by race, compared with the uterus or cervix, those originating from the ovary were unequally distributed in Caucasians (84.4% versus 69.6%, 67.7%; p < 0.001). The incidence of uterine carcinosarcoma steadily increased over time, from 2.2 in 2000 to 5.5 in 2016 (per 1,000,000), while cervical or ovarian carcinosarcoma showed no significant difference in incidence. The five-year CSS rates based on the site of origin (uterus, cervix, and ovary) were 39.9%, 33.1%, and 25.8%, respectively. The incidence rates of gynecologic carcinosarcoma, especially uterine carcinosarcoma, are gradually increasing. Although uterine carcinosarcoma is associated with a higher incidence than the others, it has a better prognosis compared with ovarian and cervical carcinosarcoma. The survival rates were worst in ovarian carcinosarcoma.
Influence of PHA Substrate Surface Characteristics on the Functional State of Endothelial Cells
Authors: Ryltseva Galina A. , Dudaev Alexey E. , Menzyanova Natalia G. , Volova Tatiana G. , Alexandrushkina Natalia A. , Efimenko Anastasia Yu. , Shishatskaya Ekaterina I.
Published: February 2023

The needs of modern regenerative medicine for biodegradable polymers are wide and varied. Restoration of the viability of the vascular tree is one of the most important components of the preservation of the usefulness of organs and tissues. The creation of vascular implants compatible with blood is an important task of vascular bioengineering. The function of the endothelial layer of the vessel, being largely responsible for the development of thrombotic complications, is of great importance for hemocompatibility. The development of surfaces with specific characteristics of biomaterials that are used in vascular technologies is one of the solutions for their correct endothelialization. Linear polyhydroxyalkanoates (PHAs) are biodegradable structural polymeric materials suitable for obtaining various types of implants and tissue engineering, having a wide range of structural and physicomechanical properties. The use of PHA of various monomeric compositions in endothelial cultivation makes it possible to evaluate the influence of material properties, especially surface characteristics, on the functional state of cells. It has been established that PHA samples with the inclusion of 3-hydroxyhexanoate have optimal characteristics for the formation of a human umbilical vein endothelial cell, HUVEC, monolayer in terms of cell morphology as well as the levels of expression of vinculin and VE-cadherin. The obtained results provide a rationale for the use of PHA copolymers as materials for direct contact with the endothelium in vascular implants.
Experimental Validation of a Multi-Purpose Exhaust System Designed for a Naval Propulsion Group
Authors: Dumitrescu Oana , Drăgan Valeriu , Gherman Bogdan , Olaru Daniel , Macrișoiu Nicolae , Nicoară Răzvan
Published: February 2023

This paper describes the effort of designing an unconventional exhaust manifold for a marine gas turbine engine, with an integrated passive ventilation port for cooling the engine housing. The study is part of a larger program to substitute the propulsion gas turbines for the T22R defense frigate and make the proper aerodynamic adaptations. The system in question is unique, in the sense that it uses the exhaust gas momentum to entrain outside air and ventilate the engine enclosure. In achieving this, RANS computation was used to test various concepts and dimensions for the ventilation system. Based on these analyses, the design that provided adequate air circulation with minimum pressure losses was chosen and the parts were integrated in the overall assembly. The experimental campaign performed on the entire aero-package showed good synergies of the ventilation system with the other adaptations and the engine itself. Performance was evaluated with pressure and temperature probes distributed around the aero-package and were found to be within 3.5% of the data predicted by CFD. This brings further studies closer to a technology readiness level vital for insitu testing on board the ship itself.
Cross-Slope Transport by a Mesoscale Anticyclone in the Northern South China Sea
Authors: Lin Xiayan , Wang Guixi , Han Guoqing , Liu Yu , Zhang Han , Liao Xiaomei , Ji Qiyan
Published: February 2023

Cross-slope eddies play an important role in the exchange of water, salt, heat, nutrients, chlorophyll, phytoplankton and other biogeochemical elements between basin and shelf in the South China Sea. The cross-slope transport process by a mesoscale anticyclonic eddy is studied by ROMS model and satellite data. The 1000 m isobath was considered as a proxy for the slope. The anticyclone shows different features at different places on the slope: (a) the volume transport at the northeast of the slope was off-slope, while at the southwest was on-slope; (b) both on and off-slope transports were greatly enhanced during the cross-slope process, and gradually weakened after crossing the slope. The total cross-slope water transport was 5.97 Sv, which was higher than the along-slope component with −0.58 Sv. The Eulerian results also showed that enhanced cross-slope transport was related to the distance between the eddy and slope, the eddy radius, and the eccentricity of the eddy. The offline passive tracer experiment showed that particles were floating up during and after the crossing process, mainly due to the strong Ekman pumping.
Optimal Allocation of Biomass Distributed Generators Using Modified Hunger Games Search to Reduce CO2 Emissions
Authors: Nassef Ahmed M. , Houssein Essam H. , Rezk Hegazy , Fathy Ahmed
Published: February 2023

Biomass is a renewable energy source because it is contained in organic material such as plants. This paper introduces a modified hunger games search for solving global optimization and biomass distributed generator problems. The hunger search algorithm is a very recent optimization algorithm. Despite its merits, it still needs some modifications. The proposed approach includes a new binary τ-based crossover strategy with satisfaction fulfillment step mechanisms. This new algorithm is designed to improve the original hunger games search algorithm by addressing some of its shortcomings, specifically, in solving problems related to global optimization such as finding the best possible solutions for biomass distributed generators. To assess the power of the new approach, its performance was evaluated on the IEEE CEC’2020 test suite against five recent and competitive algorithms. This comparison process included applying the Wilcoxon sign rank and Friedman statistical tests. Reducing the system losses and enhancing the network’s voltage profile are two main issues in the stability of radial distribution networks. Optimal allocation of biomass distributed generators in radial distribution networks can not only improve their stability but also guarantee good service to the customers. Consequently, this research work suggests an effective strategy based on the proposed approach to produce the optimal positions, sizes, and power factors of the biomass distributed generators in the network. Accordingly, the target is to mitigate the network’s active power loss such that the power flow and the bus voltage have to be maintained at their standard limits. Three distribution networks were considered for validating the superiority of the new proposed algorithm. These networks are the IEEE 33-bus, IEEE 69-bus, and IEEE 119-bus. The obtained results were compared with the gravitational search algorithm, whale optimization algorithm, grey wolf optimizer, Runge Kutta method, and the original hunger search algorithm. The new approach outperformed the other considered approaches in obtaining the optimal parameters, which mitigated the power loss to 11.6300, 5.2291, and 145.489 kW, with loss reduction of 94.49%, 97.68%, and 88.79% for the three networks, respectively.
Cooperative Path Tracking for Swarm of MASSs Based on Consensus Theory
Authors: Li Xuewei , Gao Miao , Kang Zhen , Chen Xiangyu , Zeng Xi , Chen Shuai , Sun Haixin , Zhang Anmin
Published: February 2023

At present, marine autonomous surface ships (MASSs) play a huge role in marine shipping, surveying and mapping, safeguarding the rights and interests of marine space, and other maritime tasks. The cooperative operation of a swarm of MASSs can extend the scope of operation of the MASSs, and thus allow them to carry out more complex tasks. Path tracking is an important problem for the control of a swarm of MASSs. In this paper, the control of underactuated MASSs is decoupled, to control the heading and speed, respectively. First of all, in the path tracking, the improved arc LOS guidance law is introduced, and the heading torque controller is designed, so that the MASS can track the reference path efficiently and accurately. Then the single-path guided path tracking without formation of the swarm of MASSs is studied, the reference path of the swarm center tracking is defined, and the heave thrust controller of the swarm of MASSs is designed based on consensus theory, so that the surge velocity of the MASS can tend towards consistentcy, and finally converge to the desired speed. Finally, the effectiveness of the proposed control strategy is verified by two groups of simulation experiments.
Underwater Reverberation Suppression via Attention and Cepstrum Analysis-Guided Network
Authors: Hao Yukun , Wu Xiaojun , Wang Huiyuan , He Xinyi , Hao Chengpeng , Wang Zirui , Hu Qiao
Published: February 2023

Active sonar systems are one of the most commonly used acoustic devices for underwater equipment. They use observed signals, which mainly include target echo signals and reverberation, to detect, track, and locate underwater targets. Reverberation is the primary background interference for active sonar systems, especially in shallow sea environments. It is coupled with the target echo signal in both the time and frequency domain, which significantly complicates the extraction and analysis of the target echo signal. To combat the effect of reverberation, an attention and cepstrum analysis-guided network (ACANet) is proposed. The baseline system of the ACANet consists of a one-dimensional (1D) convolutional module and a reconstruction module. These are used to perform nonlinear mapping and to reconstruct clean spectrograms, respectively. Then, since most underwater targets contain multiple highlights, a cepstrum analysis module and a multi-head self-attention module are deployed before the baseline system to improve the reverberation suppression performance for multi-highlight targets. The systematic evaluation demonstrates that the proposed algorithm effectively suppresses the reverberation in observed signals and greatly preserves the highlight structure. Compared with NMF methods, the proposed ACANet no longer requires the target echo signal to be low-rank. Thus, it can better suppress the reverberation in multi-highlight observed signals. Furthermore, it demonstrates superior performance over NMF methods in the task of reverberation suppression for single-highlight observed signals. It creates favorable conditions for underwater platforms, such as unmanned underwater vehicles (UUVs), to carry out underwater target detection and tracking tasks.
Drill Cuttings Disposal Efficiency in Offshore Oil Drilling
Authors: Cherepovitsyn Alexey , Lebedev Andrey
Published: February 2023

The relevance of the study lies in the fact that with the depletion of conventional oil and gas reserves and an increase in the global demand for hydrocarbons, the focus of the industrial sector is gradually shifting towards the resources of the Arctic, which have tremendous potential for development. However, the current industrial policy has to take into account the concept of sustainable development, or harmony between economy, ecology, and society. Therefore, the extraction of raw materials must obey the principles of the circular economy, which aims to generate closed-loop cycles that maximize the use of the resources extracted and minimize waste generation so as not to destroy fragile ecosystems. It is necessary to drill wells for the discovery of a hydrocarbon deposit on the shelf, which entails the generation of a tremendous amount of complex waste., The use of disposal methods for drilling cuttings, which must be disposed of economically and with environmental reliability, is required to solve the problem. This study compares two methods of disposing of drilling waste. Statistical modeling results and a review of the literature show that the most effective method from both economic and environmental points of view is the thermomechanical cleaning of cuttings on site. This article discusses the advantages and disadvantages of both methods. It also evaluates drilling waste management prospects and opportunities for Russian offshore fields.
Comparative Transcriptomics Profiling of Perennial Ryegrass Infected with Wild Type or a ΔvelA Epichloë festucae Mutant Reveals Host Processes Underlying Mutualistic versus Antagonistic Interactions
Authors: Rahnama Mostafa , Maclean Paul , Fleetwood Damien J. , Johnson Richard D.
Published: February 2023

Epichloë species form bioprotective endophytic symbioses with many cool-season grasses, including agriculturally important forage grasses. Despite its importance, relatively little is known about the molecular details of the interaction and the regulatory genes involved. VelA is a key global regulator in fungal secondary metabolism and development. In previous studies, we showed the requirement of velA for E. festucae to form a mutualistic interaction with Lolium perenne. We showed that VelA regulates the expression of genes encoding proteins involved in membrane transport, fungal cell wall biosynthesis, host cell wall degradation, and secondary metabolism, along with several small-secreted proteins in Epichloë festucae. Here, by a comparative transcriptomics analysis on perennial ryegrass seedlings and mature plants, which are endophyte free or infected with wild type (mutualistic interaction) or mutant ΔvelA E. festucae (antagonistic or incompatible interaction), regulatory effects of the endophytic interaction on perennial ryegrass development was studied. We show that ΔvelA mutant associations influence the expression of genes involved in primary metabolism, secondary metabolism, and response to biotic and abiotic stresses compared with wild type associations, providing an insight into processes defining mutualistic versus antagonistic interactions.
Assessment of Antifungal Pharmacodynamics
Authors: Howard Alex , Hope William
Published: February 2023

Pharmacokinetic-pharmacodynamic (PK-PD) analysis is of central importance to the progress of an antifungal agent into clinical use. It is crucial to ensure that preclinical studies give the best possible prediction of the way drugs are likely to behave in a clinical setting. This review details the last 30 years of progress in terms of disease model design, efficacy outcome selection and translational modelling in antifungal PK-PD studies. The principles of how PK-PD parameters inform current clinical practice are also discussed, including a review of how these apply to existing and novel agents.
Comparative Genomics of Histoplasma capsulatum and Prediction of New Vaccines and Drug Targets
Authors: Almeida Paula Cristina Silva , Roque Bruno Stelmastchuk , Felice Andrei Giacchetto , Jaiswal Arun Kumar , Tiwari Sandeep , Azevedo Vasco , Silva-Vergara Mario León , de Castro Soares Siomar , Ferreira-Paim Kennio , Fonseca Fernanda Machado
Published: February 2023

Histoplasma capsulatum is a thermodymorphic fungus that causes histoplasmosis, a systemic mycosis that presents different clinical manifestations, ranging from self-limiting to acute lung infection, chronic lung infection and disseminated infection. Usually, it affects severely immunocompromised patients although immunocompetent patients can also be infected. Currently, there are no vaccines to prevent histoplasmosis and the available antifungal treatment presents moderate to high toxicity. Additionally, there are few options of antifungal drugs. Thus, the aim of this study was to predict possible protein targets for the construction of potential vaccine candidates and predict potential drug targets against H. capsulatum. Whole genome sequences from four previously published H. capsulatum strains were analyzed and submitted to different bioinformatic approaches such as reverse vaccinology and subtractive genomics. A total of four proteins were characterized as good protein candidates (vaccine antigens) for vaccine development, three of which are membrane-bound and one is secreted. In addition, it was possible to predict four cytoplasmic proteins which were classified as good protein candidates and, through molecular docking performed for each identified target, we found four natural compounds that showed favorable interactions with our target proteins. Our study can help in the development of potential vaccines and new drugs that can change the current scenario of the treatment and prevention of histoplasmosis.
A New Multi-Dimensional Framework for Start-Ups Lifespan Assessment Using Bayesian Networks
Authors: Valaei Mohammadreza , Khodakarami Vahid
Published: February 2023

As historical data are typically unavailable for a start-up, risk assessment is always complex and challenging. Traditional methods are incapable of capturing all facets of this complexity; therefore, more sophisticated tools are necessary. Using an expert-elicited Bayesian networks (BNs) methodology, this paper aims to provide a method for combining diverse sources of information, such as historical data, expert knowledge, and the unique characteristics of each start-up, to estimate the default rate at various stages of the life cycle. The proposed method not only reduces the cognitive error of expert opinion for a new start-up but also considers the learning feature of BNs and the effect of lifespan when updating default estimations. In addition, the model considers the impact of investors’ risk appetite. Furthermore, the model can rank the most effective risk factors at various stages. The receiver operating characteristic (ROC) curve was utilized to assess the model’s explanatory power. Moreover, three distinct case studies were used to demonstrate the model’s capabilities.
Corn Land Extraction Based on Integrating Optical and SAR Remote Sensing Images
Authors: Meng Haoran , Li Cunjun , Liu Yu , Gong Yusheng , He Wanying , Zou Mengxi
Published: February 2023

Corn is an important food crop worldwide, and its yield is directly related to Chinese food security. Accurate remote sensing extraction of corn can realize the rational application of land resources, which is of great significance to the sustainable development of modern agriculture. In the field of large-scale crop remote sensing classification, single-period optical remote sensing images often cannot achieve high-precision classification. To improve classification accuracy, multiple time series image combinations have gradually been adopted. However, due to the influence of cloudy and rainy weather, it is often difficult to obtain complete time series optical images. Synthetic aperture radar (SAR) data are imaged by microwaves, which have strong penetrating power and are not affected by clouds. A critical way to solve this problem is to use SAR images to compensate for the lack of optical images and obtain a complete time series image in the corn-growing season. However, SAR images have limited wavelengths and cannot provide important wavelengths, such as visible light bands and near-infrared information. To solve this problem, this study took Zhaodong City, a vital corn-planting base in China, as the research area; took GF-6/GF-3 and Sentinel-1/Sentinel-2 as remote sensing data sources; designed12 classification scenarios; analyzed the best classification period and the best time series combination of corn classification; studied the influence of SAR images on the classification results of time series images; and compared the classification differences between GF-6/GF-3 and Sentinel-1/Sentinel-2. The results show that the classification accuracy of time series combinations is much higher than that of single-period images. The polarization characteristics of SAR images can improve the classification accuracy with time series images. The classification accuracy of GF series images from China is obviously higher than that of Sentinel series images. The research performed in this paper can provide a reference for agricultural classification by using remote sensing data.
Spatial-Temporal Simulation of Carbon Storage Based on Land Use in Yangtze River Delta under SSP-RCP Scenarios
Authors: Li Mengyao , Luo Hongxia , Qin Zili , Tong Yuanxin
Published: February 2023

Land use change could affect the carbon sink of terrestrial ecosystems, implying that future carbon storage could be estimated by simulating land use patterns, which is of great significance for the ecological environment. Therefore, the patterns of future land use and carbon storage under the combination scenarios of different Shared Socioeconomic Pathway (SSP) and Representative Concentration Pathway (RCP) of the Yangtze River Delta were simulated by introducing weight matrices into the Markov model and combining the PLUS and InVEST models. The results revealed that the woodland expands greatly during 2020–2060 under the SSP1-RCP2.6 scenario, and the carbon storage of 2060 is at a high level with an estimated value of 5069.31 × 106 t and an average annual increase of 19.13 × 106 t, indicating that the SSP1-RCP2.6 scenario contributes to the improvement of carbon storage. However, the area of built-up land is increasing under the SSP5-RCP8.5 scenario, and the estimated value of carbon storage is 3836.55 × 106 t, with an average annual decrease of 11.69 × 106 t, indicating that the SSP5-RCP8.5 scenario negatively affects carbon sink. Besides, the SSP2-RCP4.5 scenario causes almost no effect on land use change and carbon storage. The above results can help policymakers manage land use patterns and choose the best development scenario.