bluuebunny/crossref_metadata_2025 huggingface dataset:
“We Must Sit Like This and Discuss”: Introducing Needs Ranking as an Innovative Qualitative Methodology for Engaging Disengaged Youth
Authors: Majee Wilson , Wegner Lisa
Published: October 2023

Giving a voice to youth and promoting their health and well-being is essential for successful future communities. However, millions of youth, particularly in low-to middle-income countries, are misunderstood, lack a voice, and face many challenges, which maintains their disengagement. As approaches for youth engagement evolve, this paper presents an innovative, qualitative methodology to engage vulnerable youth (NEETs – acronym for youth who are Not in Education, Employment or Training) in identifying and ranking their needs and giving them a voice to contribute to prevention programming. Drawing on principles of empowerment theory, the paper expands on the popular youth-adult partnerships’ literature by injecting a new engagement approach that focuses on (i) using a literature-based community needs assessment, (ii) engaging vulnerable youth in small group discussion to rank their needs, (iii) comparing rankings between groups, (iv) creating spaces for youth to include other pressing issues not identified in the literature, and (iv) comparing rankings across surrounding communities. The ideas presented demonstrate that the Needs Ranking activity, as an engagement and data collection tool, may be particularly relevant in gathering viewpoints and opinions of underserved youth. These youth are traditionally not well represented through the more conventional and common methods currently employed in research and community development such as local government community development board meetings and needs assessments, and research interviews. The paper also creates awareness around the Needs Ranking activity as a viable and verifiable qualitative approach in youth engagement.
Ethical Dilemmas, Rewards, and Lessons for the Future: Conducting Participatory Photographic Research With Asylum Seekers
Authors: James Michelle L.
Published: October 2023

This paper draws on my experience of organising a photovoice project with asylum seekers to outline the ethical dilemmas and rewards of planning and facilitating a participatory, creative research project with a hard-to-reach, potentially vulnerable population. It offers lessons learnt and useful insights for others considering a similar approach to data collection. The photo project was utilised to explore the impact of involvement with community-based social protection on the self-reported wellbeing of asylum seekers in the United Kingdom. It was also designed to test the efficacy of the photovoice research methodology to produce simple, impactful findings useful for researcher, research participant and policymaker, empowering those who have often had little voice in community development to inform policy discussions. The paper is written from the author’s perspective, adopting a layered approach that utilises fieldnotes, quotes from project participants, and exhibition attendee feedback to provide a rounded description of the project. It pays particular attention to ethical dilemmas concerning project access, recruitment and communication and the complexity of juggling academic ethical rigour with a community-led approach across cultural and linguistic borders. It highlights how power dynamics can be experienced in the research environment entangled with issues of co-researcher dignity, ownership, and vulnerability. It also details the positive outcomes of the photovoice project in relation to collaborative knowledge creation, empathetic understanding, and advocacy opportunities. The author concludes by offering a summary of the key lessons learnt through the project and their implications for future research.
Theory of Planned Behaviour and Sexting Intention of College Student
Authors: Dodaj Arta , Sesar Kristina , Bošnjak Lucijana , Vučić Martina
Published: October 2023

The theory of planned behavior (TPB) is a widely used framework for predicting behavior. Considering that technology supported sexual behavior (e.g., sexting) is widespread among emerging adults, it is logical to assume that it is driven by existing social norms, accepted standards of behavior, and the opinions of others, all of which are core constructs of TPB. Therefore, the purpose of this study is to examine whether intention to sext can be predicted by the constructs of the theory of planned behavior. A quantitative cross-sectional study was conducted with 314 emerging adults aged 18–29 years who completed a self-report questionnaire online. The modified Theory of Planned Behavior Questionnaire for the sexting context, which measures attitudes toward sexting, subjective norms, and perceived behavioral control, and sexting intention, was used. The results of the path analyses mainly confirmed the model proposed by the Theory of planned behavior. Of the three TPB core factors, only subjective norm did not significantly have effect on sexting intention. Our results show that attitude, normative beliefs, and control directly predict sexting intention, and control beliefs indirectly predict sexting intention via perceived behavioral control, partially confirming the sequential relationship proposed by the Theory of planned behavior.
Will DACA Recipients Return to Their Birth Countries If DACA Is Ended?
Authors: Smith Robert Courtney
Published: October 2023

Executive Summary

Will recipients of Deferred Action for Childhood Arrivals (DACA) leave the United States if a legal challenge succeeds in ending this important program? The fate of over 600,000 current DACA recipients may depend on answers to this question in one expert report and one online survey question, which a Texas District Court found to show “a quantifiable percentage” would leave. Had the District Court in its DACA ruling not credited these sources, Texas’ lawsuit could have collapsed, and DACA would persist. The issue of whether DACA recipients will leave if DACA is ended will matter as this case is litigated, most likely to be decided by the Supreme Court. Drawing on research on immigrant return, DACA recipients, survey questions, priming, and intention-behavior gap, this paper argues that this expert report and the single online survey question used by the District Court cannot support the inferences regarding the return of DACA recipients to their birth countries. Rather, relevant research points toward DACA recipients staying in the United States, even if DACA were terminated. The paper recommends offering DACA recipients and long term undocumented residents a short path to legal status and citizenship, reassessing whether these two sources can support the conclusions drawn by the District Court; and analyzing the benefits accruing from DACA to Texas and all Texans, including US citizen children of DACA recipients, in assessing the claimed injury to the state.
Beyond the Classroom: A Case Study on the Relationship Between Education as a Public Good, Social Justice and Critical Pedagogy
Authors: Ghatak Neha , Chaudhary Rajat
Published: October 2023

The idea of education being a transformative force is central to the discourse of education. But with the evolution of societies at a rapid pace, the idea of education being a ‘public good’ stands challenged and needs to be further analysed in relation to contemporary societies. This article argues that the understanding of public goods stemming from the discipline of economics is inadequate to define the needs of the contemporary education systems. The article highlights that the idea/element of ‘social justice’ is central to understand the concept of education as a ‘public good’, and even though Indian policies on education have an element of social justice, the practice of it needs reimagining. Building on evidence from the ‘Bihar Mentorship Project’, which was an action research project at the Centre for Budget and Policy Studies, Bengaluru, the article establishes that ‘critical pedagogy’ can become a vital tool to actualise the idea of social justice in education systems and, hence, is at the core of imagining education as a ‘public good’.
Tuning nanofiltration membrane performance: OH–MoS2 nanosheet engineering and divalent cation influence on fouling and organic removal
Authors: Mallya Deepak Surendhra , Yang Guoliang , Lei Weiwei , Muthukumaran Shobha , Baskaran Kanagaratnam
Published: October 2023

Natural organic matter (NOM) present in surface water causes severe organic fouling of nanofiltration (NF) membranes employed for the production of potable water. Calcium (Ca2+) and magnesium (Mg2+) are alkaline earth metals present in natural surface water and severely exacerbate organic fouling owing to their ability to cause charge neutralization, complexation, and bridging of NOM and the membrane surface. Hence, it is of practical significance to engineer membranes with properties suitable for addressing organic fouling in the presence of these cations. This study employed OH-functionalized molybdenum disulphide (OH–MoS2) nanosheets as nanofillers via the interfacial polymerization reaction to engineer NF membranes for enhanced removal of NOM and fouling mitigation performance. At an optimized concentration of 0.010 wt.% of OH–MoS2 nanosheet, the membrane was endowed with higher hydrophilicity, negative charge and rougher membrane morphology which enhanced the pure water permeance by 46.33% from 11.2 to 16.39 L m−2 h−1 bar−1 while bridging the trade-off between permeance and salt selectivity. The fouling performance was evaluated using humic acid (HA) and sodium alginate (SA), which represent the hydrophobic and hydrophilic components of NOM in the presence of 0, 0.5, and 1 mM Ca2+ and Mg2+, respectively, and the performance was benchmarked with control and commercial membranes. The modified membrane exhibited normalized fluxes of 95.09% and 93.26% for HA and SA, respectively, at the end of the 6 h filtration experiments, compared to the control membrane at 89.71% and 74.25%, respectively. This study also revealed that Ca2+ has a more detrimental effect than Mg2+ on organic fouling and NOM removal. The engineered membrane outperformed the commercial and the pristine membranes during fouling tests in the presence of 1 mM Ca2+ and Mg2+ in the feed solution. In summary, this study has shown that incorporating OH–MoS2 nanosheets into membranes is a promising strategy for producing potable water from alternative water sources with high salt and NOM contents.
Suspension of the penis – dissection, anatomical description and highlighting of anatomical risks in sectioning the suspensory ligaments
Authors: Filipoiu Florin-Mihail , Ion Radu-Tudor , Filipoiu Zoran Florin , Tulin Adrian-Daniel , Enciu Octavian , Enyedi Mihaly
Published: October 2023

Background

The suspension of the penis is provided by two ligaments: fundiform and suspensory. These ligaments are sectioned during some augmentative surgical procedures. The structure, the relations and the variability of these ligaments have been demonstrated. The penile neurovascular bundle and its relationships have also been emphasized. A clear knowledge of these details should ensure a reduction of the risk of surgical injury during augmentation procedures.

Results

We dissected the ligaments providing the suspension of the penis in 7 formalized corpses. We identified, for each of the ligaments, the origin, the insertion and the relations. The dissection pieces were photographed and the images obtained were discussed upon. We described the variability of the anatomical distribution and highlighted the relations with the vascular and nervous structures for each of these ligaments. The anatomical variability of the fascia and the relations with the base of the penis were also emphasized. For the suspensory ligament, we identified three groups of fibers through which it is attached to the penile body.

Conclusions

The dissections were conducted in layers, corresponding to the operative steps for the penile augmentation procedures. We believe that our study highlights the anatomical basis necessary to safely perform these surgeries. The study contributes to the description of the anatomical variability of the ligaments and logically presents details that contribute to preventing most surgical incidents.
Interruption time series analysis using autoregressive integrated moving average model: evaluating the impact of COVID-19 on the epidemic trend of gonorrhea in China
Authors: Li Yanyan , Liu Xingyan , Li Xinxiao , Xue Chenlu , Zhang Bingjie , Wang Yongbin
Published: October 2023

Background

Interrupted time series (ITS) analysis is a growing method for assessing intervention impacts on diseases. However, it remains unstudied how the COVID-19 outbreak impacts gonorrhea. This study aimed to evaluate the effect of COVID-19 on gonorrhea and predict gonorrhea epidemics using the ITS-autoregressive integrated moving average (ARIMA) model.

Methods

The number of gonorrhea cases reported in China from January 2005 to September 2022 was collected. Statistical descriptions were applied to indicate the overall epidemiological characteristics of the data, and then the ITS-ARIMA was established. Additionally, we compared the forecasting abilities of ITS-ARIMA with Bayesian structural time series (BSTS), and discussed the model selection process, transfer function, check model fitting, and interpretation of results.

Result

During 2005–2022, the total cases of gonorrhea were 2,165,048, with an annual average incidence rate of 8.99 per 100,000 people. The highest incidence rate was 14.2 per 100,000 people in 2005 and the lowest was 6.9 per 100,000 people in 2012. The optimal model was ARIMA (0,1, (1,3)) (0,1,1)12 (Akaike’s information criterion = 3293.93). When predicting the gonorrhea incidence, the mean absolute percentage error under the ARIMA (16.45%) was smaller than that under the BSTS (22.48%). The study found a 62.4% reduction in gonorrhea during the first-level response, a 46.47% reduction during the second-level response, and an increase of 3.6% during the third-level response. The final model estimated a step change of − 2171 (95% confidence interval [CI] − 3698 to − 644) cases and an impulse change of − 1359 (95% CI − 2381 to − 338) cases. Using the ITS-ARIMA to evaluate the effect of COVID-19 on gonorrhea, the gonorrhea incidence showed a temporary decline before rebounding to pre-COVID-19 levels in China.

Conclusion

ITS analysis is a valuable tool for gauging intervention effectiveness, providing flexibility in modelling various impacts. The ITS-ARIMA model can adeptly explain potential trends, autocorrelation, and seasonality. Gonorrhea, marked by periodicity and seasonality, exhibited a downward trend under the influence of COVID-19 intervention. The ITS-ARIMA outperformed the BSTS, offering superior predictive capabilities for the gonorrhea incidence trend in China.
Hand grip strength, vitamin D status, and diets as predictors of bone health in 6–12 years old school children
Authors: Alghadir Ahmad H. , Gabr Sami A. , Iqbal Amir
Published: October 2023

Background

Vitamin D and calcium-rich foods, exposure to sunlight, and physical activities (PA) play a pivotal role in promoting the production of sufficient vitamin D and improving grip strength needed for better bone health among school children.

Purpose

This study aimed to determine the effects of hand grip muscle strength (HGS), vitamin D in addition to diets, and PA on bone health status among 6–12 years old schoolchildren.

Methods

This study was based on a cross-sectional observational design, which was descriptive in nature. A diverse sample of 560 elementary school children aged 6–12 years old were invited to participate in this descriptive cross-sectional study. The Dual-Energy X-Ray Absorptiometry (DXA), QUS technique, and ACTi graph GT1M accelerometer were used respectively as a valid tools to identify BMD, BMC, and other parameters of bone health like c-BUA values and bone stiffness (SI), and physical activity (PA) of all individuals participated in this study. In addition, a hydraulic dynamometer was used to measure hand grip strength among the participants. Moreover, an immunoassay technique was used to measure the serum levels of vitamin 25(OH)D level, and bone metabolism markers; NTX, DPD, Ca, and sBAP in all participants. Bone loss (osteoporosis) was cross-sectionally predicted in 19.64% of the total population, most of whom were girls (14.3% vs. 5.4% for boys; P = 0.01). Compared to boys, the incidence of osteoporosis was higher and significantly correlated in girls with lower HGS, deficient vitamin D, inadequate vitamin D and Ca intake, greater adiposity, poor PA, and lower sun exposure. Also, in girls, lower vitamin 25(OH)D levels, and poor HGS were shown to be significantly associated with lower values of BMD, BMC, SI, and higher values of bone resorption markers; NTX, DPD, and sBAP and lower serum Ca than do in boys. The findings suggested that deficient vitamin D, lower HGS, adiposity, PA, and sun exposure as related risk factors to the pravelence of bone loss among school children, particularly in girls. In addition, these parameters might be considered diagnostic non-invasive predictors of bone health for clinical use in epidemiological contexts; however, more studies are required.
Effects of neuraxial or general anaesthesia on postoperative adverse events in oldest-old patients (aged 90 years and older) with intertrochanteric fractures: a retrospective study
Authors: Guo Wei-dong , Li Yue , Li Jia-hui , Han Feng , Huang Guo-shun
Published: October 2023

Background

To retrospectively analyse postoperative adverse events in oldest-old patients (aged 90 years and older) with intertrochanteric fractures treated under various anaesthetic techniques.

Methods

A total of 153 consecutive patients participated in this study, of which 127 patients who underwent surgery with neuraxial anaesthesia or general anaesthesia for intertrochanteric fractures between October 2019 and October 2022 were eligible and evaluated. They were divided into the neuraxial anaesthesia and general anaesthesia groups. The demographic characteristics and postoperative adverse events were compared between the two groups.

Results

A total of 13 patients (10.24%), including 6 in the neuraxial anaesthesia group (8.22%) and 7 in the general anaesthesia group (12.96%), died within 30 days after surgery. No significant differences between the two groups were observed. Postoperative delirium occurred in 40 patients (31.49%), including 17 (23.29%) in the neuraxial anaesthesia group and 23 (42.59%) in the general anaesthesia group; there was a significant difference between the two groups [P = 0.02, odds ratio (OR) = 0.41]. The other postoperative adverse events, including heart failure, acute stroke, acute myocardial infarction, pulmonary disease, anaemia, deep vein thrombosis, hypoproteinaemia, and electrolyte disorders, were not significantly different between the two groups.

Conclusion

Our data suggest that different anaesthesia methods do not affect the incidence of adverse events, such as death within 30 days after surgery in oldest-old patients with intertrochanteric fractures. However, more patients developed delirium after surgery in the general anaesthesia group (23, 42.59%) than in the neuraxial anaesthesia group (17, 23.29%); this may indicate that spinal anaesthesia reduces the incidence of postoperative delirium (P = 0.02, OR = 0.41).

Trial registration

Retrospectively registered.
Evaluation of compressive strength, surface microhardness, solubility and antimicrobial effect of glass ionomer dental cement reinforced with silver doped carbon nanotube fillers
Authors: Hamdy Tamer M.
Published: October 2023

Background

Conventional glass ionomer cements (GICs) are currently the most widely used dental cements due to their chemical bonding into tooth structure, release of fluoride, and ease of manipulation and usage. One of their drawbacks is their low mechanical properties and high solubility. Carbon nanotubes (CNTs) could be utilized in dentistry due to their several potential applications. CNTs can be used as fillers to reinforce polymers or other materials. Additionally, silver (Ag) nanoparticles are highly effective at preventing dental biofilm and enhancing mechanical properties.

Objectives

The aim of the present in vitro study is to evaluate the compressive strength, surface microhardness, solubility, and antimicrobial effect of the conventional GIC reinforced with manual blending of 0.01 wt.% Ag doped CNT fillers.

Methods

The control group was prepared by mixing dental GIC powder with their liquid. The innovatively reinforced dental GIC group was prepared by incorporating 0.01 wt.% Ag doped CNT fillers into the GIC powder prior to liquid mixing. Chemical characterization was performed by XRF. While, physical characterization was done by measuring film thickness and initial setting time.

The compressive strength, surface microhardness, solubility, and antimicrobial effect againstStreptococcus mutansbacteria using an agar diffusion test were measured. The data was statistically analyzed using independent samplet-tests to compare mean values of compressive strength, surface microhardness, solubility, and antimicrobial activity (p ≤ 0.05).

Results

The results revealed that innovative reinforced GIC with 0.01 wt.% Ag doped CNT fillers showed higher mean compressive strength, surface microhardness, and antimicrobial effect values than the conventional GIC control group; there was no significant difference between different groups in relation to the solubility test (P ≤ 0.05).

Conclusion

The innovatively reinforced GIC with 0.01 wt.% Ag doped CNT fillers had the opportunity to be used as an alternative to conventional GIC dental cements.
Identifying factors that affect the use of health information technology in the treatment and management of hypertension
Authors: Faezi Aysan , Afshar Hadi lotfnezhad , Rahimi Bahlol
Published: October 2023

Background

We conducted this study with the aim of identifying factors that affect the use of health information technology in the treatment and management of hypertension.

Methods

This paper is a descriptive-analytic study conducted in 2022. To obtain relevant articles, databases including Scopus, Web of Science, IEEE, and PubMed were searched and the time period was between 2013 and 2022. Based on the review of similar articles, a five-point Likert scale checklist was developed in the second phase. The statistical population of the present study was specialist physicians (N = 40) and patients (N = 384). In order to analyze the data, SPSS Statistics 24 was used. To analyze the data obtained from the checklist, we used summary statistics (mean and standard deviation).

Results

As a result of the review literature process, 50 papers were screened, that based we can distinguish motivational and inhibitory factors affecting the use of health information technology in hypertension management. Indeed, Motivational factors and inhibitory factors can be classified into five groups: organizational, economic, technical, personal, and legal/moral factors. Based on the results of the checklist, the factors that were identified as most influential on motivation and inhibitory patients and specialist physicians’ to use of health information technology to manage and treat hypertension.

Conclusion

Utilizing technologies for hypertension, its management can be improved by identifying motivating and inhibiting factors. Our approach can improve the acceptability of these technologies, save costs, reduce long-term complications of hypertension, and improve patient quality of life.
Behavioural economic interventions to reduce health care appointment non-attendance: a systematic review and meta-analysis
Authors: Werner Kalin , Alsuhaibani Sara Abdulrahman , Alsukait Reem F. , Alshehri Reem , Herbst Christopher H. , Alhajji Mohammed , Lin Tracy Kuo
Published: October 2023

Background

Appointment non-attendance – often referred to as “missed appointments”, “patient no-show”, or “did not attend (DNA)” – causes volatility in health systems around the world. Of the different approaches that can be adopted to reduce patient non-attendance, behavioural economics-oriented mechanisms (i.e., psychological, cognitive, emotional, and social factors that may impact individual decisions) are reasoned to be better suited in such contexts – where the need is to persuade, nudge, and/ or incentivize patients to honour their scheduled appointment. The aim of this systematic literature review is to identify and summarize the published evidence on the use and effectiveness of behavioural economic interventions to reduce no-shows for health care appointments.

Methods

We systematically searched four databases (PubMed/Medline, Embase, Scopus, and Web of Science) for published and grey literature on behavioural economic strategies to reduce no-shows for health care appointments. Eligible studies met four criteria for inclusion; they were (1) available in English, Spanish, or French, (2) assessed behavioural economics interventions, (3) objectively measured a behavioural outcome (as opposed to attitudes or preferences), and (4) used a randomized and controlled or quasi-experimental study design.

Results

Our initial search of the five databases identified 1,225 articles. After screening studies for inclusion criteria and assessing risk of bias, 61 studies were included in our final analysis. Data was extracted using a predefined 19-item extraction matrix. All studies assessed ambulatory or outpatient care services, although a variety of hospital departments or appointment types. The most common behaviour change intervention assessed was the use of reminders (n = 56). Results were mixed regarding the most effective methods of delivering reminders. There is significant evidence supporting the effectiveness of reminders (either by SMS, telephone, or mail) across various settings. However, there is a lack of evidence regarding alternative interventions and efforts to address other heuristics, leaving a majority of behavioural economic approaches unused and unassessed.

Conclusion

The studies in our review reflect a lack of diversity in intervention approaches but point to the effectiveness of reminder systems in reducing no-show rates across a variety of medical departments. We recommend future studies to test alternative behavioural economic interventions that have not been used, tested, and/or published before.
Construction and validation of a competency model for hospital operation assistant of public hospitals in China: a cross‑sectional study
Authors: Gao Jia , Tian Meirong , Liu Jun , Chen Jingjing , Zhang Lei , Wang Xiaodong , Yan Ling , Liu Qiwang , Wen Jin
Published: October 2023

Background

Hospital operation assistant (HOA) plays an important role in promoting the operation effectiveness and efficiency of hospital. China, as a resource-poor country, urgently needs to train HOA talent. The purpose of this study is to construct and validate a competency model for HOA, which can be used as a tool to select and train HOAs.

Methods

Basic competency items were first constructed through literature review combined with the job analysis of HOA. Then, a questionnaire survey conducted on more than 300 hospital operation management-related staff was used to assess the importance of competency items. Exploratory factor analysis, structural equation model and second-order confirmatory factor analysis were used to construct and validate the competency model of HOA.

Results

A total of 23 items were identified as critical to HOA capability, which were further divided into three factors: professional skills, professional knowledge and personality traits. The structural equation model showed that the standardized path coefficients of the three factors were 0.86, 0.82 and 0.98. The competency model passed strict fitting effect tests in several aspects, including root-mean-square error of approximation (RMSEA) = 0.077 (< 0.080), standardized root mean square residual (SRMR) = 0.062 (< 0.080), comparative fit index (CFI) = 0.927 (> 0.900) and Tucker-Lewis index (TLI) = 0.918 (> 0.900), which showed that the fitting validity of the model was ideal. The composite reliability (CR), average variance extracted (AVE) and correlation coefficients of all factors were within the standard range, which showed that the construction validity and discrimination validity of the model were ideal.

Conclusion

Our study indicates that the competency model of HOA is an instrument with appropriate fit validity, construct validity and discriminant validity, which can provide criteria for selecting and training HOAs.
Comparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region
Authors: Gombert Sara , Jahn Kirsten , Pathak Hansi , Burkert Alexandra , Schmidt Gunnar , Wiehlmann Lutz , Davenport Colin , Brändl Björn , Müller Franz-Josef , Leffler Andreas , Deest Maximilian , Frieling Helge
Published: October 2023

Background

Bisulfite sequencing has long been considered the gold standard for measuring DNA methylation at single CpG resolution. However, in recent years several new approaches like nanopore sequencing have been developed due to hints for a partial error-proneness of bisulfite sequencing. Since these errors were shown to be sequence-specific, we aimed to verify the methylation data of a particular region of the TRPA1 promoter from our previous studies obtained by bisulfite sequencing.

Methods

We compared methylation rates determined by direct bisulfite sequencing and nanopore sequencing following Cas9-mediated PCR-free enrichment.

Results

We could show that CpG methylation levels above 20% corroborate well with our previous data. Within the range between 0 and 20% methylation, however, Sanger sequencing data have to be interpreted cautiously, at least in the investigated region of interest (TRPA1 promotor region).

Conclusion

Based on the investigation of the TRPA1- region as an example, the present work can help in choosing the right method out of the two current main approaches for methylation analysis for different individual settings regarding many factors like cohort size, costs and prerequisites that should be fulfilled for each method. All in all, both methods have their raison d’être. Furthermore, the present paper contains and illustrates some important basic information and explanation of how guide RNAs should be located for an optimal outcome in Cas9 mediated PCR free target enrichment.
Performance of ultra-sensitive malaria rapid diagnostic test to detect Plasmodium falciparum infection in pregnant women in Kinshasa, the Democratic Republic of the Congo
Authors: Kabalu Tshiongo Japhet , Luzolo Flory , Kabena Melissa , Kuseke Lise , Djimde Moussa , Mitashi Patrick , Lumbala Crispin , Kayentao Kassoum , Menting Sandra , Mens Petra F. , Schallig Henk D. F. H. , Lutumba Pascal , Tinto Halidou , Muhindo Mavoko Hypolite , Maketa Vivi
Published: October 2023

Background

Low peripheral parasitaemia caused by sequestration of Plasmodium falciparum in the placenta hampers the diagnosis of malaria in pregnant women, leading to microscopy or conventional rapid diagnostic tests (RDTs) false-negative results. Although mainly asymptomatic, maternal malaria remains harmful to pregnant women and their offspring in endemic settings and must be adequately diagnosed. Ultra-sensitive RDTs (uRDTs) are thought to be more sensitive than RDTs, and their diagnostic performance was assessed in the current study in pregnant women living in Kinshasa, a stable malaria transmission area in the Democratic Republic of the Congo.

Methods

To assess and compare the diagnostic performances of both RDTs and uRDTs, 497 peripheral blood samples were tested using microscopy and quantitative polymerase chain reaction (qPCR) as the index and the reference tests, respectively. The agreement between the different diagnostic tests assessed was estimated by Cohen's Kappa test.

Results

The median parasite density by qPCR was 292 p/μL of blood [IQR (49.7–1137)]. Using qPCR as the reference diagnostic test, the sensitivities of microscopy, RDT and uRDT were respectively [55.7% (95% CI 47.6–63.6)], [81.7% (95%CI 74.7–87.3)] and [88% (95% CI 81.9–92.6)]. The specificities of the tests were calculated at 98.5% (95% CI 96.6–99.5), 95.2% (95% CI 92.5–97.2) and 94.4% (95% CI 91.4–96.6) for microscopy, RDT and uRDT, respectively. The agreement between qPCR and uRDT was almost perfect (Kappa = 0.82). For parasite density (qPCR) below 100 p/µL, the sensitivity of RDT was 62% (95% CI 47.1–75.3) compared to 68% (95% CI 53.3–80.4) for uRDT. Between 100 and 200 p/µL, the sensitivity of RDT was higher, but still lower compared to uRDT: 89.4% (95% CI 66.8–98.7) for RDT versus 100% (95% CI 82.3–100) for uRDT. In both cases, microscopy was lower, with 20% (95% CI 10–33.7) and 47.3% (95% CI 24.4–71.1) respectively.

Conclusions

uRDT has the potential to improve malaria management in pregnant women as it has been found to be slightly more sensitive than RDT in the detection of malaria in pregnant women but the difference was not significant. Microscopy has a more limited value for the diagnosis of malaria during the pregnancy, because of its lower sensitivity.
Cumulative live birth rates with autologous oocytes plateau with fewer number of cycles for each year of age > 42
Authors: Seifer David B. , Wang Sarah F. , Frankfurter David
Published: October 2023

Objective

To disaggregate the Society for Assisted Reproductive Technology Clinic Outcome Reporting System (SART CORS) age category of “ > 42” and compare age-stratified cumulative live birth rates (CLBR) > 42 years old.

Design

Retrospective cohort study of autologous linked ART cycles.

Setting

United States (US) National ART Database.

Patient(s)

Women > 42 years old without a history of prior ART cycles who underwent ART between 2014–2020 as reported to the SART CORS database.

Intervention(s)

Disaggregate the SART CORS age category of “ > 42” into age-stratified cumulative live birth rates (CLBR).

Main Outcome Measure(s)

Age-stratified cumulative live birth rates (CLBR) for women ≥ 43 years old.

Results

Between 2014–2020, 24,650 women > 42 years old without history of prior ART underwent 58,132 cycles, resulting in 1,982 live births. Women ages 43, 44, 45, 46, 47, 48, 49, ≥ 50 achieved maximal CLBR of 9.7%, 8.6%, 5.0%, 3.6%, 2.5%, 1.5%, 2.7%, 1.3%, respectively. CLBR for women between 43–45 were significantly higher compared to those 46 and older (p < 0.05). Among women 46 and older, CLBR were not significantly different. Women ages 43 and 44 did not exhibit a significant increase in CLBR beyond the 5th cycle. Age 45 and 46 reached CLBR plateau by the 3rd cycle. Age ≥ 47 CLBR plateaued after the first cycle. After adjusting for age, race/ethnicity, BMI, nulliparity, etiology of infertility, number of oocytes retrieved, embryos transferred, blastocyst transfer, use of ICSI, PGT, and ART treatment cycle number, there was no association between markers of ovarian reserve (day 3 FSH and random AMH levels) and live birth for women > 42.

Conclusions

While CLBR of autologous cycles from women 42 or younger generally plateau by cycle number 5, age-stratified cycles from women > 42 plateau after fewer cycles to maximize CLBR. Patient and physician expectations for maximum CLBR beyond 42 may be practically based on fewer planned cycles before reaching an age-specific CLBR plateau than may have been previously expected.
Preeclampsia-associated lncRNA FEZF1-AS1 regulates cell proliferation and apoptosis in placental trophoblast cells through the ELAVL1/NOC2L axis
Authors: Zhao Xudong , Su Fengyun , Guo Qing , Tao Xiuhong , Wang Huifeng , Wang Hongling , Li Qinwen , Zhang Wangmeng
Published: October 2023

Background

LncRNAs have been shown to be involved in and control the biological processes of multiple diseases, including preeclampsia (PE). The impairment of trophoblast cell proliferation is recognized as a significant anomaly contributing to the development of PE. LncRNA FEZF1-AS1 was found downregulated in placental tissues of PE patients. However, the precise regulatory mechanism of FEZF1-AS1 in placental trophoblast proliferation and apoptosis remains unclear.

Results

In this study, we conducted an investigation into the expression levels of FEZF1-AS1 and NOC2L in placental tissues obtained from patients diagnosed with PE. Subsequently, we employed CCK-8 and EdU assays to quantify cell proliferation, while TUNEL staining and western blot for apoptosis-related protein detection to assess apoptosis. Furthermore, the interactions between FEZF1-AS1 and ELAVL1, as well as NOC2L and ELAVL1, were confirmed through the implementation of RIP and RNA pull-down assays. We found a downregulation of lncRNA FEZF1-AS1 and NOC2L in placental tissues of PE patients. Overexpression of FEZF1-AS1 or NOC2L resulted in increased cell proliferation and inhibition of apoptosis, whereas knockdown of FEZF1-AS1 or NOC2L had the opposite effect. In addition, lncRNA FEZF1-AS1 stabilized NOC2L mRNA expression by interacting with ELAVL1. Moreover, partial reversal of the effects of FEZF1-AS1 overexpression on cell proliferation and apoptosis was observed upon suppression of ELAVL1 or NOC2L.

Conclusions

PE related lncRNA FEZF1-AS1 could regulate apoptosis and proliferation of placental trophoblast cells through the ELAVL1/NOC2L axis.
Prospective study of pain and patient outcomes in the emergency department: a tale of two pain assessment methods
Authors: Ku Nai-Wen , Cheng Ming-Tai , Liew Chiat Qiao , Chen Yun Chang , Sung Chih-Wei , Ko Chia-Hsin , Lu Tsung-Chien , Huang Chien-Hua , Tsai Chu-Lin
Published: October 2023

Background

Accurate pain assessment is essential in the emergency department (ED) triage process. Overestimation of pain intensity, however, can lead to unnecessary overtriage. The study aimed to investigate the influence of pain on patient outcomes and how pain intensity modulates the triage’s predictive capabilities on these outcomes.

Methods

A prospective observational cohort study was conducted at a tertiary care hospital, enrolling adult patients in the triage station. The entire triage process was captured on video. Two pain assessment methods were employed: (1) Self-reported pain score in the Taiwan Triage and Acuity Scale, referred to as the system-based method; (2) Five physicians independently assigned triage levels and assessed pain scores from video footage, termed the physician-based method. The primary outcome was hospitalization, and secondary outcomes included ED length of stay (EDLOS) and ED charges.

Results

Of the 656 patients evaluated, the median self-reported pain score was 4 (interquartile range, 0–7), while the median physician-rated pain score was 1.5 (interquartile range, 0–3). Increased self-reported pain severity was not associated with prolonged EDLOS and increased ED charges, but a positive association was identified with physician-rated pain scores. Using the system-based method, the predictive efficacy of triage scales was lower in the pain groups than in the pain-free group (area under the receiver operating curve, [AUROC]: 0.615 vs. 0.637). However, with the physician-based method, triage scales were more effective in predicting hospitalization among patients with pain than those without (AUROC: 0.650 vs. 0.636).

Conclusions

Self-reported pain seemed to diminish the predictive accuracy of triage for hospitalization. In contrast, physician-rated pain scores were positively associated with longer EDLOS, increased ED charges, and enhanced triage predictive capability for hospitalization. Pain, therefore, appears to modulate the relationship between triage and patient outcomes, highlighting the need for careful pain evaluation in the ED.
Work accident effect on the use of psychotropic drugs: the case of benzodiazepines
Authors: Barnay Thomas , Baudot François-Olivier
Published: October 2023

Background

A work accident constitutes a shock to health, likely to alter mental states and affect the use of psychotropic drugs. We focus on the use of benzodiazepines, which are a class of drugs commonly used to treat anxiety and insomnia. Prolonged use can lead to dependence. Our objective is to determine the extent to which work accidents lead to benzodiazepine use and overuse (i.e. exceedance of medical guidelines).

Method

We use a two-step selection model (the Heckman method) based on data from the French National Health Data System (Système National des Données de Santé, SNDS). Our study sample includes all general plan members who experienced a single work accident in 2016 (and not since 2007). This sample includes 350,000 individuals in the work accident group and more than 1.1 million people randomly drawn from the population without work accidents from 2007 to 2017 (the non-work accident group).

Results

The occurrence of a work accident leads to an increase in benzodiazepine use and overuse the following year. The selection model shows a clear influence of the accident on the use probability (+ 39%), but a very slight impact on the risk of overuse among users (+ 1.7%), once considered the selection effect. The effect on overuse risk is higher for more severe accidents and among women.

Conclusion

The increase in the risk of benzodiazepine overuse is due to an increase in the likelihood of using benzodiazepines after a work accident that leads to overuse, rather than an increase in likelihood of overuse among people who use benzodiazepines. Results call for targeting the first-time prescription to limit the risk of overuse after a work accident.
Efficient design of a wideband tunable microstrip filtenna for spectrum sensing in cognitive radio systems
Authors: Elabd Rania H. , Hussein Amr H.
Published: October 2023

This paper presents a novel design of a compact, wideband tunable microstrip filtenna system for effective spectrum sensing in cognitive radio (CR) applications. The proposed filtenna structure has a total bandwidth of $$1.63\,\text{GHz}$$

1.63

GHz

and flexible frequency scanning design throughout the frequency range from $$1.93\,\text{ to }\,3.56\,\text{ GHz}$$

1.93

to

3.56

GHz

with high selectivity and narrow bandwidths ranging from $$39.9\,\text{to}53\,\text{MHz}$$

39.9

to
53

MHz

. Frequency tuning is accomplished electrically via integrating a varactor diode into the filtenna construction. The filtenna is realized on a Rogers TMM4 substrate with $$h=1.52\,\text{mm}$$

h
=
1.52

mm

thickness and relative dielectric constant of $${\varepsilon }_{r}=4.5$$

ε
r

=
4.5

with dimensions of $$(25\times 35)\, {\text{mm}}^{2}$$

(
25
×
35
)

mm

2

. The obtained gain and efficiency of the filtenna ranged from $$0.7$$

0.7

to $$2.26\,\text{dBi}$$

2.26

dBi

and 49% to 60%, respectively, within the tuning range. Simple biasing circuitry, wideband operation, and compact planar structure are distinctive and appealing aspects of the design. For the manufactured prototypes, a significant level of agreement is found between the simulated and measured results in terms of scattering parameter $${\text{S}}_{11}$$

S
11

and radiation patterns at different operating frequencies.
Influence of adolescents’ and parental dietary knowledge on adolescents’ body mass index (BMI), overweight/obesity in 2004–2015: a longitudinal study
Authors: Cai Zhengjie , Jiang Ke , Wang Tiankun , Li Shengping , Xian Jinli , Zhao Yong , Shi Zumin
Published: October 2023

Objective

The global epidemic of overweight/obesity in children and adolescents poses a significant public health threat. This longitudinal study aims to investigate the relationship between adolescents’ and their parents’ dietary knowledge and overweight/obesity among adolescents in China.

Methods

Data were collected from the China Health and Nutrition Survey (CHNS) in 2004, 2006, 2009, 2011 and 2015. Overweight/obesity was defined according to the International Obesity Task Force (IOTF) cut-off for body mass index (BMI). A set of questions were used to assess the dietary knowledge scores of both adolescents and their parents during face-to-face interviews. Mixed effect models were used to analyze the data.

Results

A total of 2035 adolescents aged 12–17 years were included in the data analysis. The mean BMI increased from 19.2 in 2004 to 20.5 in 2015, with a significant increase in the prevalence of overweight and obesity from 6.8% and 0.5% in 2004 to 15.1% and 7.8% in 2015, respectively. Adolescents with medium-score dietary knowledge were less likely to be overweight/obese compared to those with low-score dietary knowledge (OR (95% CI): 0.20 (0.05–0.80), P < 0.05). However, there was no association between parental dietary knowledge and adolescents’ BMI or overweight/obesity (P > 0.05). Additionally, a significant interaction between adolescents’ dietary knowledge score and education in relation to adolescents’ BMI (P for interaction < 0.05).

Conclusion

Adolescents with medium dietary knowledge were less likely to be overweight/obese than those with low knowledge, but no association was found with parental knowledge. Promoting nutritional education and healthy eating habits is vital to prevent overweight/obesity.
Significant perioperative parameters affecting postoperative complications within 30 days following craniotomy for primary malignant brain tumors
Authors: Yang Yao-Chung , Chen Yao-Shen , Liao Wei-Chuan , Yin Chun-Hao , Lin Yung-Shang , Chen Meng-Wei , Chen Jin-Shuen
Published: October 2023

Background

The occurrence of postoperative complications within 30 days (PC1M) of a craniotomy for the removal of a primary malignant brain tumor has been associated with a poor prognosis. However, it is still unclear to early predict the occurrence of PC1M. This study aimed to identify the potential perioperative predictors of PC1M from its preoperative, intraoperative, and 24-h postoperative parameters.

Methods

Patients who had undergone craniotomy for primary malignant brain tumor (World Health Organization grades III and IV) from January 2011 to December 2020 were enrolled from a databank of Kaohsiung Veterans General Hospital, Taiwan. The patients were classified into PC1M and nonPC1M groups. PC1M was defined according to the classification by Landriel et al. as any deviation from an uneventful 30-day postoperative course. In both groups, data regarding the baseline characteristics and perioperative parameters of the patients, including a new marker-kinetic estimated glomerular filtration rate, were collected. Logistic regression was used to analyze the predictability of the perioperative parameters.

Results

The PC1M group included 41 of 95 patients. An American Society of Anesthesiologists score of > 2 (aOR, 3.17; 95% confidence interval [CI], 1.19–8.45;p = 0.021), longer anesthesia duration (aOR, 1.16; 95%CI, 0.69–0.88;p < 0.001), 24-h postoperative change in hematocrit by >  − 4.8% (aOR, 3.45; 95%CI, 1.22–9.73;p = 0.0019), and 24-h postoperative change in kinetic estimated glomerular filtration rate of < 0 mL/min (aOR, 3.99; 95%CI, 1.52–10.53;p = 0.005) were identified as independent risk factors for PC1M via stepwise logistic regression analysis. When stratified according to the age of ≥ 65 years (OR, 11.55; 95%CI, 1.30–102.79;p = 0.028), the reduction of kinetic estimated glomerular filtration rate was more robustly associated with a higher risk of PC1M.

Conclusions

Four parameters were demonstrated to significantly influence the risk of PC1M in patients undergoing primary malignant brain tumor removal. Measuring and verifying these markers, especially kinetic estimated glomerular filtration rate, would help early recognition of PC1M risk in clinical care.