bluuebunny/crossref_metadata_2025 huggingface dataset:
Indicators of the dimensions of trust (and mistrust) in early primary care practice: A qualitative study
Authors: Zgurzynski Mary , O'Reilly-Jacob Monica , Duggan Ashley P. , Vicini Andrea , Shaughnessy Allen
Published: November 2022

Background
Trust occurs when a person feels they can be vulnerable to others because of the sincerity, benevolence, truthfulness and sometimes the competence they perceive. This project examines the various types of trust expressed in written reflections of developing healthcare clinicians. Our goal is to understand the roles trust plays in residents’ self-examination and to offer insight from relationship science to inform the teaching and clinical work for better trust in healthcare.
Methods
We analyzed 767 reflective writings of 33 residents submitted anonymously, to identify explicit or implicit indicators attention to trust or relationship development. Two authors independently coded the entries based on inductively identified dimensions. Three authors developed a final coding structure that was checked against the entries. These codes were sorted into final dimensions.
Results
We identified 114 written reflections that contained one or more indicators of trust. These codes were compiled into five code categories: Trust of self/trust as the basis for confidence in decision making; Trust of others in the medical community; Trust of the patient and its effect on clinician; Assessment of the trust of them exhibited by the patient; and Assessment of the effect of the patient’s trust on the patient’s behavior.
Discussion
Broadly, trust is both relationship-centered and institutionally situated. Trust is a process, built on reciprocity. There is tacit acknowledgement of the interplay among what the residents do is good for the patient, good for themselves, and good for the medical institution. A focus on moments in which trust is experienced or missed, as well as on types of trust, misses this complexity.
Conclusion
A greater awareness of how trust is present or absent could lead to a greater understanding and healthcare education for beneficial effects on clinicians’ performance, personal and professional satisfaction, and improved quality in patients’ interactions.
Comparison of CT values in traditional trajectory, traditional cortical bone trajectory, and modified cortical bone trajectory
Authors: Liu Dongshan , Kahaer Alafate , Wang Yixi , Zhang Rui , Maiaiti Abulikemu , Maimaiti Xieraili , Zhou Zhihao , Shi Wenjie , Cui Zihao , Zhang Tao , Li Longfei , Rexiti Paerhati
Published: November 2022

Background: To compare the CT values and length of the screw tracks of traditional trajectory (TT), cortical bone trajectory (CBT), and modified cortical bone trajectory (MCBT) screws and investigate the effects on the biomechanics of lumbar fixation.
Methods: CT scan data of 60 L4 and L5 lumbar spine were retrieved and divided into 4 groups (10 male and 10 female cases in the 20-30 years old group and 20 male and 20 female cases in the 30-40 years old group). 3-dimentional (3D) model were established using Mimics 19.0 for each group and the placement of three techniques was simulated on the L4 and L5, and the part of the bone occupied by the screw track was set as the region of interest (ROI). The mean CT value and the actual length of the screw track were measured by Mimics 19.0.
Results: The CT values of ROI for the three techniques were significantly different between the same gander in each age group (P＜0.05). The difference of screw track lengths for CBT and MCBT in the male and female is significant (P＜0.05). Conclusions: According to the CT values of the three screw tracks: MCBT > CBT > TT, the MCBT screw track has greater bone-screw surface strength and longer screw tracks than CBT, which is easier to reach the anterior column of the vertebral body contributing to superior biomechanical properties.
Daily work variability in falls prevention of hospitalized patients: Nursing team’s perception
Authors: de QUADROS Deise Vacario , WACHS Priscila , de MAGALHÃES Ana Maria Müller , SEVERO Isis Marques , TAVARES Juliana Petri , PAI Daiane DAL
Published: November 2022

Background: the identification of safety incidents and establishment of systematic methodologies in health services to reduce risks and provide quality care was implemented by The World Health Organization. These safety incidents allowed the visualization of a wide panorama, ranging from preventable incidents to adverse events with catastrophic outcomes for patients. In this scenario, the issue of fall(s) is inserted, which, despite being a preventable event, can lead to several consequences for the patient, family and the healthcare system, being the second cause of death by accidental injury worldwide, this study aims to identify the variability inherent in the daily work in fall prevention, the strategies used by professionals to deal with it and the opportunities for improvement of the management of work-as-imagined.
Design: an exploratory and descriptive study, of qualitative approach, through process modeling and semi-structured interviews.
Methods: was conducted in a public university hospital in southern Brazil, following four steps: modeling of the prescribed work; identification of falls; modeling of the daily work and reflections on the gap between work-as-done and work-as-imagined. Medical records, management reports, notification records, protocols, and care procedures were consulted for modeling the work process and semi-structured interviews were conducted with 21 Nursing professionals. The study was conducted between March 2019 and December 2020.
Findings: from July 2018 to July 2019, 447 falls occurred, 2.7% with moderate to severe injury. The variability occurred in the orientation of the companion and in the assurance of the accompanied patient's de-ambulation. The professionals identified individual strategies to prevent falls, the importance of multi-professional work, learning with the work team, the colleague’s expertise, as well as suggesting improvements in the physical environment.
Conclusion: this study addressed the need for fall prevention in the hospital setting as one of the main adverse events that affect patients. The identification of the variability inherent to the work allows professionals to identify opportunities for improvement, understand the risks to which patients are subjected and develop the perception of fall risk as a way to reduce the gap between work-as-imagined and work-as-done.
Information Use, Seeking Behaviors, and Barriers to Evidence-Based Information at a Large Teaching Hospital in the UK
Authors: Lagojda Lukasz , O'Connell Bridget , Brown Anna , Dekker Natasha S. den
Published: November 2022

Background: To better understand the needs of service users, this study explored the information use and the factors affecting access to evidence-based information by clinicians in a primary care setting.
Methods: An online survey was distributed to all staff via SurveyMonkey®. Data were curated in Microsoft Excel and managed using SPSS v26. Graphs were generated using GraphPad PRIMS.
Results: Ninety-three per cent of the surveyed clinicians used evidence-based information in practice, primarily for patient care and education. Clinicians employed a range of seeking behaviors to retrieve this information, which varied based on how often this information was needed. The barriers to evidence-based information varied between clinical professions but ‘time’ was the most consistent factor. The belief of evidence-based practice being not part of the job role was the overwhelming factor for not using evidence-based information.
Conclusions: The findings of this study are consistent with previous reports describing significant impact of time and access to IT resources on whether search for evidence-based information and provided us with useful insights for improving our services.
Cd uptake mechanisms by hyperaccumulator Bidens pilosa L. roots under different salinity and pH levels
Authors: Wang Siqi , Dai Huiping , Cui Shuang , Ji Dandan , Skuza Lidia , Li Lianzhen , Grzebelus Dariusz , Wei Shuhe
Published: November 2022

Bidens pilosa L. has been confirmed to be a potential Cd hyperaccumulator by some researchers, but the dynamic and real-time uptake of Cd2+ influx by B. pilosa root apexes was a conundrum up to now. The aim of our study was to investigate the effects of salinity and pH variations on the characteristics of Cd2+ influx around the root apexes of B. pilosa. The tested seedlings of B. pilosa were obtained by sand culture experiments in a greenhouse after one month from germination, and the Cd2+ influxes from the root apex of B. pilosa under Cd treatments with different salinity and pH levels were determined with application of non-invasive micro-test technology (NMT). The results showed that Cd2+ influxes at 300 µm from the root tips decreased under Cd treatments with 5 mM and 10 mM NaCl, as compared to Cd stress alone. However, Cd treatments with 2.5 mM NaCl had little effect on the net Cd2+ influxes, as compared to Cd treatments alone. Importantly, Cd treatments at pH = 4.0 markedly increased Cd2+ influxes in roots, and Cd treatment at pH = 7.0 had no significant effect on the net Cd2+ influxes with compared to Cd treatments at pH = 5.5. Results also showed that Cd treatments with 10 mM NaCl significantly decreased concentrations of chlorophyll (Chl) a and b in leaves and root vigor of B. pilosa relative to Cd treatments alone, while there were no significant differences between Cd treatments with 2.5 mM NaCl and Cd treatments alone. But root vigor was inhibited significantly under Cd treatments with 5 mM and 10 mM NaCl. A significant increase of root vigor was observed in Cd treatments at pH = 4.0, as compared to pH = 5.5. The Cd treatments with high and medium concentrations of NaCl inhibited the uptake of Cd by B. pilosa roots, and affected the Chl and root vigor further. But the Cd treatments at pH = 4.0 could promote the Cd uptake and root vigor. Our results revealed the uptake mechanisms of B. pilosa as a potential phytoremediator under different salinity and pH levels combined with Cd contamination, and provided a new idea for screening ideal hyperaccumulator and constructing evaluation system.
Probing the binding nature and stability of highly transmissible mutated variant Alpha to Omicron of SARS-CoV-2 RBD with ACE2 via molecular dynamics simulation
Authors: Ramakrishnan Jaganathan , Chinnamadhu Archana , Suresh Suganya , Poomani Kumaradhas
Published: November 2022

As of now there is no approved drug available as a causative agent of COVID-19. To resolve this issue, quickly identify a repurposed drug and develop new vaccines are the most promising solutions as it is the time-consuming process to design a new drug. As a therapeutic development, some vaccines and some repurposed drugs were approved to prevent people from SARS-CoV-2 infections during this pandemic period; all these medicines are made available based on the first structure of SARS-CoV-2 reported in late 2019 from Wuhan, China. Since then, vividly it is known that due to serious mutations in this virus, often its structure has been modified and emerged as the new types of variants; evidently, the new variants are the most dangerous and spreading very fast. SARS-CoV-2 variants are becoming the significant challenge to the current pandemic situation. Hence, the detailed structure of these variants is necessary to design new drugs and vaccines to inhibit the mutated SARS-CoV-2 variants. Present study analyzed the binding mode of receptor binding domain (RBD) of SARS-CoV-2 with the human angiotensin converting enzyme 2 (ACE2) using molecular dynamics simulation up to 500 ns. Notably, in some variants found an unusual binding mode of RBD with ACE2 forming different interactions; this was confirmed from the comparison of interaction between RBD-ACE2 of all variants with the wild type structure. Binding free energy values confirm that they exhibit high binding affinity towards ACE2. From this analysis, it is confirmed that the variations in the sequence of SARS-CoV-2 spike protein altered the binding mode which led to the high transmissibility caused new infections. This in-silico study on mutated variants alpha to omicron of SARS-CoV-2 RBD with ACE2 insights their binding mode, binding affinity and stability. This information is useful to design potential drug and vaccine to cure the devastating disease COVID-19.
Dysmenorrhea, intermenstrual bleeding, and irregularities post COVID-19 vaccination and associated factors: a retrospective observational study
Authors: Khan Ghadeer , Althubaiti Alaa , Al-Shrif Amal , Alsayed Zahra , Jifree Hatim
Published: November 2022

Background
coronavirus SARS-CoV-2 disease 2019 (COVID-19) vaccines have provided a fertile ground for research, especially with the unprecedented misinformation spread. One of the most reported side effects of the vaccine among women has been menstrual disturbances. In this paper, we aim to investigate the extent of the COVID-19 vaccine effects on the menstrual cycle as well as identify the associated factors that predispose an individual to dysmenorrhea, intermenstrual bleeding, change in flow volume, or irregularity post vaccine.
Methods
Any woman of menstruating age who received at least one dose of the COVID-19 vaccine and currently resides in Saudi Arabia was invited to complete an online questionnaire. The questionnaire compared menstruation characteristics—regularity, volume, intermenstrual bleeding, and dysmenorrhea—before receiving the COVID-19 vaccine with after, taking into consideration possible predisposing factors such as polycystic ovarian syndrome (PCOS), contraceptives, or period blocking pills.
Results
Women without PCOS experienced less changes in dysmenorrhea post COVID-19 vaccine compared to those with PCOS (23.88% vs 27.78% p-value = 0.045). Women using contraceptives were about two times more likely to experience volume changes compared to those not using contraceptives (OR = 2.09, 95%CI 1.23–3.57, p-value = 0.006). COVID-19 vaccine skeptics were about three times more likely to perceive changes in regularity post vaccine compared to non-skeptics (OR = 2.96, 95%CI, 1.79–4.90, p-value < 0.001). They were, also, 3 times more likely to perceive changes in volume post vaccine (OR = 3.04, 95%CI = 1.95–4.74, p-value < 0.001).
Conclusion
COVID-19 vaccine skepticism has a significant association with perceived post vaccine menstrual disturbances. Therefore, public education is crucial to combat the spread of misinformation and controversy surrounding the vaccines.
Who are the older adults' users or non-users of the Internet before the pandemic? Analysis of the EpiFloripa Ageing Cohort Study (2009-2019)
Authors: Quialheiro Anna , Rech Cassiano Ricardo , Silvestre Michelli , Boing Antonio Fernando , d´Orsi Eleonora
Published: November 2022

This ageing demographic transformation has been happening simultaneously with technological immersion, especially internet use in communication and health monitoring applications. This study's primary aim was to characterize the Internet's socioeconomic and demographic factors of internet users and non-users. The second aim was to analyze which factor, modifiable or not, influenced the incidence of older adults' Internet use over time. This study was done with 722 EpiFloripa Ageing Cohort Study follow-up of older adults. The outcome is internet use according to waves 1 to 3, and the exposures are sociodemographic. It described the characteristics of internet users and non-users, and it used a longitudinal analysis model (Generalized Estimating Equations) to estimate incidence rate ratios (IRR) with 95% confidence intervals. Internet users were younger, with higher education and higher income than non-users over time. Most non-users of the Internet were women (54%) and white (93%), with an average of 66.4 years old and 14 years of study. The incidence of internet use was 11,4% in Wave 2 and 23.0% in Wave 3 and was 80% lower in older adults 80 years and over compared to other age groups. Regarding schooling and family income, the internet use incidence was higher according to the increase in education levels and income range, except for older adults with more than 10 Brazilian minimum wage. Public policies are needed to reduce inequalities in access to the Internet and promote the digital inclusion of older adults.
Effect of PCL nanofiber mats coated with chitosan microcapsules containing cinnamon essential oil for wound healing
Authors: Osanloo Mahmoud , Noori Fariba , Tavassoli Alireza , Ataollahi Mohammad reza , Davoodi Ali , Seifalah-Zade Morteza , goodarzi Arash , Fereydouni Narges
Published: November 2022

Introduction: Cinnamon is one of the most common spices that have been studied for its anti-inflammatory, antioxidant, and antibacterial properties in wound healing. The purpose of this study was to evaluate the effectiveness of polycaprolactone nanofiber mats coated with chitosan microcapsules loaded with cinnamon essential oil on wound healing.
Material and methods: For this purpose, chitosan microcapsules containing cinnamon essential oil (µCS-CiZ) were prepared by ion gelation and PCL nanofibers by electrospinning. The size of the µCS-CiZ and the morphology of nanofibers were evaluated by DLS and FESEM methods. In order to evaluate wound healing, 48 rats in 4 groups of control, µCS-CiZ, nanofibers, and nanofibers coated with µCS-CiZ (PCL+µCS-CiZ) were examined on days 7, 14, and 21 in terms of macroscopy (wound closure rate) and histology (edema, inflammation, vascularity, fibrotic tissue, and re-epithelialization).
Results: The particle size of the µCS-CiZ and the diameter of the nanofibers were estimated at about 6.33±1.27 μm and 228 ± 33 nm, respectively. On day 21, both µCS-CiZ and PCL groups showed a significant decrease in wound size compared to the control group (p<0.001). The PCL+µCS-CiZ group also showed a significant decrease compared to the µCS-CiZ (p<0.05) and PCL groups (p<0.05). Histological results showed further reduction of edema, inflammation, and vascularity in granulation tissue, and appearance of moderate to marked fibrotic tissue in PCL+µCS-CiZ group compared with the other groups.
Conclusion: The results of the study showed that the combined use of PCL+µCS-CiZ indicates a synergistic effect in improving wound healing.
Correlative Analysis among AHP-based Scenic Beauty Estimation and Water Quality Indicators of 2 Typical Urban Constructed WQT Wetlands
Authors: Huang Yan , Li Tianjie , Jin Yang , Wu Wenting
Published: November 2022

Urban constructed water quality treatment (WQT) wetlands are often designed for removing pollutants, i.e., DO, CODCr, NH3-N, and TP. The water environment of WQT wetland waterscapes is strongly related to water quality, while scenic beauty values help achieve better water environments. To reveal correlations between several water quality indicators (WQIs) and scenic beauty indexes (SBIs), an AHP-based scenic beauty estimation (SBE) per site in 2 constructed WQT wetlands has been developed in the research. The weights of indicators are determined by the AHP voting method, which includes 3 main criteria, i.e., conditions of vegetation, physical geographic conditions, and human geographic conditions. SBIs are calculated by voting by judges from professional and unprofessional groups. WQIs of pollutants were sampled in situ during the field research simultaneously. Relationships among SBIs and WQIs are indicated by correlative analysis and a linear regression model with multiple variables. Applications for WQT wetland landscaping should be conducted on quantitative estimations in 3 aspects, i.e., follow strategies of ecological landscaping, improve water quality treatment techniques, and apply attention restoration theory. With deeper comprehension of SBIs, practical design, management, and maintenance of urban constructed WQT wetlands will be optimised.
Compression-Based Cancelable Multi-Biometric System
Authors: Elshazly Emad A. , Hashad Fatma G. , Sedik Ahmed , El-Samie Fathi E. Abd , Abdel-Salam Nariman
Published: November 2022

The issue of cybersecurity is one of the important fields which is involved in different research trends. Biometric security is one of these trends which is involved in several applications such as access control systems and online identity verification. The protection of human biometrics can be performed using both bi-directional and unidirectional encryption. The unidirectional encryption is carried out based on cancelable biometric techniques. This paper proposes a cancelable biometric system based on image composition, deep dream, and hashing techniques. The objective of the proposed system is to generate visual and text cancelable biometrics. The visual cancelable templates are generated using image composition and deep dream, while the text templates are generated using SHA hashing techniques. The proposed system is validated by multi-biometric inputs including iris, palm, face, and fingerprint biometrics. In addition, it is evaluated in both visual and text forms. The simulation results reveal that the proposed system appears a superior performance among the works which handle this problem.
Profiling of Driver Mutations Identified Rare Compound Mutations Sensitive to Second-generation EGFR-TKI in Lung Adenocarcinoma Patients
Authors: Li Jun , Zhang Cuiyun , Guan Yuping , Zheng Jiawen , Feng Junnan , Han Sile , Ma Ruijuan , Ren Pengfei , Li Shasha , Li Yi , Groen Harry J. M. , Kok Klaas , Berg Anke van den , Wei Bing , Ma Jie , Guo Yongjun , Li Hongle
Published: November 2022

Background: Lung adenocarcinoma (LUAD) is the most predominant histological subtype of lung cancer characterized by driver mutations detected in a substantial proportion of the cases. Tyrosine kinase inhibitors (TKIs) are standard care for the patients with these mutations. In this study, we evaluated the efficiency of an NGS-based 8-gene test in selecting TKIs-sensitive Chinese LUAD patients who are treatment-naïve.
Material and methods: Targeted sequencing covering the hotspot regions of eight LUAD driver genes was performed across 853 treatment-naïve LUAD patients. Logistic regression analysis was used to determine the factors associated with presence of these mutations. Genetically modified LUAD PC9 cells were established to evaluated the sensitivity of selected EGFRcompound mutations to different EGFR-TKIs in-vivo.
Results: A total of 574 single nucleotide variants, 270 InDels, 88 amplifications, and 87 rearrangements were identified in this study, with EGFR and KRAS being the most frequently mutated genes. Females, mostly life-long non-smokers, had significantly higher EGFRmutation rates than males and had a T>A conversion signature, for which the etiological factor is still unknown. Males, primarily smokers, more frequently had KRAS mutations and a smoking-associated signature of C>A conversions. Rare EGFR compound mutations identified in this study (Exon19del plus L747S/I744V and L858R plus V843I/T854A/G873) conferred genetically modified PC9 cells, constantly and consistently, more sensitive to second-generation EGFR-TKI afatinib in-vivo.
Conclusion: Over 70% of Chinese treatment-naïve LUAD patients are TKIs-targetable. Patients with rare EGFR compound mutations might conside second-generation EGFR-TKIs for treatment.
Association of blood total immunoglobulin E and eosinophils with radiological features of bronchiectasis
Authors: Ren Jiaqi , Chen Ai , Wang Jun , Chang Chun , Wang Juan , Sun Lina , Sun Yongchang Yongchang
Published: November 2022

Background: Our study aimed to investigate whether serum total IgE and blood eosinophils, were associated with radiological features of bronchiectasis in a Chinese cohort.
Methods: We retrospectively enrolled bronchiectasis patients who visited Peking University Third Hospital from Jan 1st, 2012 to Oct 7th, 2021. The clinical, laboratory and chest CT characteristics were analyzed in association with serum total IgE level and blood eosinophil count.
Results: A total of 125 bronchiectasis patients were enrolled, with 50.4% (63/125) female, and a mean age of 62.4±14.11 years. The median serum total IgE level and blood eosinophil count were 47.7 (19.8, 123.0) KU/L and 140 (90, 230) cells/µl, respectively. In patients with a higher than normal (normal range, 0-60 KU/L) total IgE (43.2%, n=54), more lobes were involved (4 (3, 5) vs 3 (2, 4), p=0.008), and mucus plugs were more common (25.9% vs 9.9%, p=0.017) on HRCT, as compared to those with a normal level of total IgE. The higher IgE group was more likely to have bilateral involvement (p=0.059), and had numerically higher Smith and Bhalla scores, but the differences were not statistically significant. In patients with an eosinophil count ≥150 cells/µl (49.6%, n=62), the number of lobes involved was greater (4 (3, 5) vs 3 (2, 4), p=0.015), and the Smith and Bhalla scores were higher (9 (5, 12) vs 6 (3, 9), p=0.009, 7 (5, 11) vs 5 (3, 9), p=0.036). The Smith score was correlated positively with the eosinophil count (r=0.207, p=0.020). Fractional exhaled nitric oxide (FeNO) was correlated with total IgE (r=0.404, p=0.001) and eosinophil count (r=0.310, p=0.014).
Conclusions: Our study demonstrated that serum total IgE and the blood eosinophil count were associated with the radiological extent and severity of bronchiectasis, necessitating further investigation on the role of T2 inflammation in structural abnormalities of this heterogeneous disease.
An Environmental Financial System for Accelerating the Development of Climate-friendly Products and Services
Authors: Pazos Jay
Published: November 2022

Global warming is becoming an existential problem, and solving it will require the broadest participation from society. One significant force available to society is the capacity of markets to redirect capital resources to solve problems. Here we propose an Environmental Financial System (EFS) to abate climate change that consists of eight principles, five stakeholders and three binding equations that define and make the system operational. EFS helps climate change abatement by developing a novel methodology for calculating the climate-friendliness of companies and finding an optimal portfolio of climate-friendly companies. The system frames the solution in ways that make it universally available, robust and easy to comprehend. EFS has two objectives: first, to supply funds to climate-friendly companies, and second, to communicate in a manner that incentivises actions and nudges patrons to modify their behaviour towards climate-friendly products, services, lifestyles and habits.
Development of a labelled-LFIA coupled with LAMP for the rapid and specific detection of B. melitensis targeting BMEI1661 gene
Authors: Ashmi Marcia , Kumar Bablu , . Sanjana , . Abhishek , Kumar Deepak , Singh Praveen
Published: November 2022

The primary cause of Brucellosis in sheep, goats, human and other animal species is B. melitensis. Despite being widely acknowledged as the gold standard method, the isolation and identification of B. melitensis cannot currently meet the criteria for early diagnostic strategies. Conventional PCR methods and immunological assays can detect B. melitensis, but their use in basic laboratories is constrained by the need for sophisticated instruments. A prompt and accurate diagnosis is essential to prevent the spread of infection to human and animals. The aim of this study was to set up a rapid and specific point of care diagnostic test for the detection of B. melitensis, which could be used routinely. Hence, a Loop mediated isothermal amplification (LAMP) coupled with lateral flow immunoassay (LFIA) was optimized for the specific detection of B. melitensis. The LAMP primers were tagged to generate product labelled with digoxigenin and biotin and the labelled LAMP amplicons were detected using LFIA with streptavidin-gold nanoparticle as a capture reagent. The assembly of gold nanoparticle at the test and control line yielded a characteristic red colour band. The sensitivity of B. melitensis LAMP-LFIA assay was 12.1fg. The assay was 100 fold more sensitive than conventional PCR and was in accordance with RT-PCR. The assay did not exhibit cross reactivity with the non-Brucella pathogens examined in this study or other Brucella species. The assay can be performed in a water bath at 65°C within 60 min and can detect upto12.1fg of genomic DNA isolated from the organism and 102 CFU/ml of B. melitensis in the spiked sample. Thus there is a great deal of potential for this assay to be utilised as a rapid field test for screening different secretions and excretions in suspected animals or their products which can tremendously reduce chances of disease transmission to both animal and human.
Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease
Authors: Qi Jie , Han Wenqi , Zhong Nier , Gou Qiling , Sun Chaofeng
Published: November 2022

Coronary heart disease is a cardiovascular disease with high morbidity and mortality. Although great progress has been made in treatment, the prognosis is still very poor. Therefore, this project aims to identify and screen potential diagnostic markers and therapeutic targets related to the progression of coronary heart disease. A total of 94 overlapping differentially expressed mRNAs and 73 differentially expressed miRNAs were identified by limma package from GSE20681, GSE12288, GSE49823 and GSE105449. Through a series of bioinformatics techniques and qPCR, we obtained 5 core miRNA-mRNA regulatory pairs, and selected miR-338-3p/RPS23 for functional analysis. Moreover, we found that RPS23 directly targets miR-338-3p by dual luciferase assay, western and qPCR. And the expression of miR-338-3p and RPS23 is negatively correlated. The AUC value of miR-338-3p is 0.847. Down-regulation of miR-338-3p can significantly inhibit the proliferation and migration of HUVEC. On the contrary, overexpression of miR-338-3p inhibited HUVEC cell apoptosis and promoted the proliferation and migration of HUVEC. In addition, overexpression of RPS23 can reverse the effect of miR-338-3p mimic on the proliferation activity of HUVECs. Overexpression of miR-338-3p significantly promotes the growth of HUVECs by down-regulating RPS23. In conclusion. The effect of miR-338-3p/RPS23 may be involved in the progression of coronary heart disease, and suggests that miR-338-3p may be a diagnostic biomarker and therapeutic target for coronary heart disease.
Analysis of risk factors for increased incidence of gallstone caused by hepatectomy: A retrospective case-control study
Authors: Zhou Jiangmin , Chen Lin , Zhang Zhiwei , Wu Biao
Published: November 2022

Background: An increased risk of gallstone has been observed in patients undergoing hepatectomy. This study attempted to analyze the risk factors of gallstone after hepatectomy.
Methods: From January 2013 to December 2016, Clinical data of 1452 eligible patients who underwent hepatectomy were consecutively reviewed. According to the imaging results including gallbladder ultrasound, CT, and MRI, all patients were divided into gallstone group and non-gallstone group. Univariate and multivariate logistic regressions were used to select indicators associated with gallstone formation among patients after hepatectomy.
Results: In the total sample of included patients, there were 341 patients with gallstone and 1147 patients without gallstone. The incidence of gallstones was (341/1452) 23.5%. The incidence of gallstones in primary liver cancer group was higher than benign liver tumor group (25.7% versus 18.9%, P=0.004). Univariate and multivariate logistic regression analysis showed that female gender, high body mass index (BMI), tumor located in S5 and severe postoperative complication were factors related to gallstone in patients with benign tumor of liver after hepatectomy. Besides, Child-Pugh B, low albumin, liver cirrhosis, and transcatheter arterial chemoembolization (TACE) after recurrence were factors related to gallstone in patients with primary liver cancer after hepatectomy.
Conclusions: Hepatectomy increased the risk of gallstone in both benign or malignant liver tumor, especially, when tumor was located in S5. TACE further increased the risk of gallstone in patients with postoperative recurrence.
Optical Multiparametric Bio-analyte Detection System: Design, Development and Noise Analysis
Authors: Basu Deeparati , Hossain Syed Minhaz , Das Jayoti
Published: November 2022

In this work, we have designed and developed a multiparametric optical detection system for bio-analyte measurement. Five interlinked optical parameters i.e. transmittance, reflectance, internal scattering, surface scattering and output power are measured simultaneously from transmitted and reflected spot images in MATLAB image processing environment. Also RGB channel information of images was studied to find further non-interlinked parameters for chromatic white light source. Detailed noise analysis of the system was performed to study the effect of individual component noise and finally a simple noise reduction method is presented. The system was tested with non-transparent and semi-transparent sensor samples for glucose sensing application and found to have quite sensitive performance inside a particular range of operation. The system was able to measure both real-time data and fixed time interval data for all the interlinked parameters simultaneously. Such sensitive, reliable and cost-effective instrument with multiparametric measurement feature has immense potential in optical biosensor field.
Improve Classification of Security Bug Reports using fasttext. A Machine Learning Based Approach
Authors: Alqahtani Sultan S.
Published: November 2022

Software developers must handle security bug reports (SBRs) before they are widely disclosed, and the system becomes vulnerable to hackers. Bug tracking systems may contain many securities-related reports which are unlabelled as SBRs. Therefore, finding unlabelled SBRs is a challenge to help security engineers identify these security issues fast and accurately. Although many methods have been proposed for classifying SBRs, challenging issues remain due to selecting an accurate and high-performance classification algorithm. This motivates us to tackle the challenges faced by the state-of-the-art SBRs classification methods by selecting a high-performance machine learning algorithm. Therefore, the main goal of this paper is to automate the process of determining which bug report can be labeled as SBR through the use of machine learning techniques. We first extracted 45,940 bug reports from publicly available datasets of five software repositories (e.g., the work of Peters et al. and Shu et al.). Second, we conducted a study on the classification of SBRs using machine learning, where we built a fasttext classifier. We then examined the accuracy of using fasttext in detecting SBRs. Our results show that fasttext can identify SBRs with an average F1 score of 0.81. Furthermore, we investigated the generalizability of identifying SBRs by applying cross-project validation, and our results show that the fasttext classifier achieves an average F1 value of 0.65. Data and results are available at https://github.com/isultane/fasttext_classifications.
Immune profiles in Bone marrow to predict induce therapy response for multiple myeloma patients
Authors: Zhaoyun Liu , Hongli Shen , Mei Han , Xianghong Zhao , Hui Liu , Kai Ding , Jia Song , Rong Fu
Published: November 2022

Purpose: To find bone marrow immune detection index which related to MM patients therapy.
Method: We analyzed ninety-three newly diagnosed MM patients retrospectively and their the stage of prognosis, the clinical and induced-therapy response datasets.
Result: In our study, Eight-color flow cytometry, a method commonly used to detect MM cell loads, was used to analyze seven bone marrow immune cell groups (B, NK, T, naive B, immature B, memory B, and plasmablast like cells). The percentage of B cells significantly decreased in stage III, compared with stage I patients, (P = 0.047, P = 0.038) according to the ISS and R-ISS. In the clinical characteristics, naive B cell numbers increased in the Hb(g/L)≥100 group, when compared with the Hb(g/L)< 100 group (P = 0.028). The immature B (P = 0.017), plasmablast like cell subgroups(P = 0.037) increased in MM patients, who achieved a very good partial response(VGPR), after four cycles of the new drug-based induced therapy. The ROC results indicated the combination of the seven immune subgroups had predictive values (AUC = 0.840; P < 0.0001).
Conclusion: comprehensively analyze seven bone marrow immune cell subgroups can be a useful approach for predicting the induce therapy response in MM patients.
A proposed novel genetic and epigenetic effects of Betulinic acid against breast cancer cells: in silico and in vitro study
Authors: Fadel Hewida H. , El-Wakeel Esraa , Donia Hanaa , El-Rahman Mohamed Abd , EL-Esseily Hadeer Adel , Sewedy Tarek El
Published: November 2022

Purpose Estrogen receptor (ER) and the Piwi-Like Protein 1 (PIWIL1) oncogene alter the expression and methylation of oncogenes and tumor suppressor genes. Potential cross-talk between ER and the methylated-DNA binding protein, Kaiso, and its downstream genes c-Myc and CDKN2A remains unexplored. Dual targeting of ER and PIWIL1 might be an excellent therapeutic approach.
Methods Initially, we used in silico molecular docking to identify potential blockers for ER and PIWIL1 among seven natural previously reported anticancer compounds. The best candidate was tested in vitro for its influence on KAISO expression by RT-PCR, promoter methylation of c-Myc and CDKN2A by methylation-specific PCR, cell cycle and apoptosis by flowcytometry, and cytotoxicity by MTT assay in the ER(+) MCF-7, and ER(−) MDA-231-MB breast cancer cell lines.
Results Betulinic acid (BA), docked ER-α and PIWIL1 perfectly at critical amino acids with the lowest binding energy. Moreover, ER and PIWIL1 docked Kaiso with root mean square deviation = 5.05 Å and 4.75 Å, respectively, at the critical amino acid Y537. Experimentally, BA caused a dose-dependent cytotoxicity in both MCF-7 and MDA-MB-231 cells (IC50= 21.3 ± 0.05 and 100 ± 0.34 µM), respectively. BA caused a significant dose-dependent G0/G1 cell cycle arrest, apoptosis induction, and upregulated Kaiso expression coincided with increased methylation of c-Myc and demethylation of CDKN2A promoter regions.
Conclusion: We are proposing a novel anticancer mechanism of action for BA via potential inhibition of Estrogen-ER-α and PIWIL1 signaling, modulation of KAISO expression and selective alterations in methylation state of c-Myc and CDKN2A key cancer-associated genes.
Socially Distanced: An Exploratory Study of the Relationships Between Delusional Ideation and Social Imagery Under the Lens of the COVID-19 Pandemic
Authors: Robertson Kennedy , Gold Ian , Veissiere Samuel , Robillard Rebecca , Solomonova Elizaveta
Published: November 2022

Purpose: Delusional ideation, defined as false beliefs and altered thinking, has long been attributed to anomalous/bizarre perceptual experiences and reasoning deficits. Recent studies, however, have challenged this view, suggesting instead that delusional ideation may have more of a social component to its phenomenology and underlying mechanisms. This study investigated associations between delusional ideation and factors of social imagery (felt presence, empathy, loneliness, and social anxiety) in healthy adults, using the COVID-19 pandemic as a natural social experiment rich in perceived threat and social isolation.
Methods: 2,200 participants completed the online “How are you coping? COVID-19 survey,” designed to measure the psychological, social, and financial impacts of the pandemic. Delusional ideation was assessed using the Peters Delusional Ideation (PDI) scale and felt presence experiences were assessed by the Other Experiences Questionnaire (OEQ). Other measures of social imagery included the Liebowitz Social Anxiety Scale (LSAS), the Interpersonal Reactivity Index (IRI) (empathy), and the UCLA Loneliness Scale.
Results: All aspects of social imagery were positively associated with delusional ideation. The strongest predictor of PDI score was felt presence (ß=.298, p<.001), followed by loneliness (ß=.222, p<.001), LSAS (social fear dimension) (ß=.135, p=.001), IRI (empathic concern scale) (ß=.101, p<.001), and IRI (empathizing with fictitious characters scale) (ß=.088, p<.001).
Conclusion: Results support the hypothesis that delusional ideation is sensitive to alterations in sociality, and delusions may involve social cognition for representing social agents. We propose that delusions and social imagery may share common mechanisms and increased propensity for imagining others may contribute to development of delusions.
Multi-Objective Based Route Selection Approach Using AOMDV in MANET
Authors: Saxena Madhvi , DUTTA SUBRATA , Neogy Sarmistha
Published: November 2022

Mobile ad hoc networks consist of mobile nodes in wireless mode. Because of ease deployment, low cost and easy communication, MANET has become one of the attractive research area in wireless communication. The challenging issues in MANET are limited energy of nodes, high mobility and dynamic topologies. These issues cause frequent link breakage due to this route discovery process reinitiated repeatedly. Many researchers gave their contribution for improving the existing protocols; still there are many scopes of improvements present. In this paper a novel routing algorithm MO-AOMDV (Multi Objective AOMDV) is proposed. In this algorithm route discovery and route selection is based on the quality of node and links, it denoted by G (V, L) where V present number of nodes and L present the link between nodes. In addition, every node has a local list where the remaining energy of node and list of neighbors is updated. It is developed and simulated in network simulator and the performance is compared with traditional AODV and AOMDV algorithm and comparing parameters are packet drop ratio, packet delivery ratio, and network lifetime.
Examination of a medial collateral ligament pie-crusting model by a finite element method
Authors: Matsuda Shogo , Hirakawa Masashi , Nagashima Yuu , Akase Hiroya , Kaku Nobuhiro , Tsumura Hiroshi
Published: November 2022

Background: Medial collateral ligament (MCL) pie-crusting is implemented to balance the soft tissue when performing total knee arthroplasty. Although studies have been conducted on pie-crusting, a virtual MCL model has not been created so far, and pie-crusting has not been examined using the finite element method (FEM). This study investigates and validates MCL models using FEM.
Methods: We developed an MCL model for finite element analysis where 19 elastic bodies (fibers) were bundled, and an adhesive part (Young's modulus of 175 kPa) was set in the gap and attached to both ends of the bone. An additional model 2 was created with nine 1 mm × 0.1 mm cuts in the center of the elastic body. Model 3 was developed considering nine and ten staggered cuts (19 total cuts) at two different locations, with a gap of 20 mm. Models 4 to 8 had 28, 38, 47, 76, and 95 cuts performed similarly. Subsequently, these models were analyzed. The left end of the bone was fixed, and rightward forces of 80 N and 120 N were applied to the models.
Results: The model was extended by 0.34 and 0.50 mm for every ten punctures added.
Conclusions: Our results suggest what model is better suited for the mechanical analysis of pie-crusting.
Nível de conhecimento e atitude dos pais ou responsáveis por escolares sobre traumatismo dentoalveolar
Authors: Mourato Ivana Tamires Pires , Ferraz Thays Maria Ramalho , Melo Jéssica Gomes Alcoforado de , Filgueira Pedro Tardelly Diniz , Magalhães Vinicius Souto , Soares Diego Moura
Published: November 2022

Introdução: O traumatismo dentoalveolar vêm sendo cada vez mais frequentes em crianças e adolescentes, acredita-se que esse trauma pode ocasionar perdas dentais irreparáveis em alguns casos. Diante disso, o sucesso ou fracasso de dentes avulsionados e reimplantados depende, portanto, de condutas rápidas e apropriadas ainda no local do acidente, sendo indispensáveis que as pessoas presentes no local tenham informações básicas suficientes para prestar os primeiros socorros. Objetivo: Este estudo avaliou o nível de conhecimento e atitudes de pais ou responsáveis por crianças em idade escolar de 6 a 12 anos. Materiais e métodos: O presente artigo trata-se de um estudo observacional, analítico, prospectivo, transversal, realizado por meio de um questionário online com 76 pais e responsáveis por escolares de 6 a 12 anos do município de Serra Talhada. Resultados: A média de conhecimento/atitudes gerais dos participantes sobre traumatismo dentoalveolar foi de 3,97 (desvio padrão = 2,22). A maioria da amostra não possuía experiência (n= 49; 64,5%) e informação prévia (n= 55; 72,4%). Menos da metade dos participantes (43,4%) acreditam que o dente avulsionado pode ser reimplantado e utilizariam o leite como meio de conservação e de transporte (9,2%) e apenas (26,3%) teriam a iniciativa de realizar o reimplante de forma imediata. Conclusão: Pais ou responsáveis por escolares de 6 a 12 anos apresentam pouco conhecimento sobre os procedimentos de urgência a serem realizados nos casos de traumatismo dentário.
Avaliação In Vitro do Potêncial Erosivo e Cariogênico de Balas Duras Dissolvidas em Saliva Artificial
Authors: Muniz Lívia Jacqueline Gomes , Santos Raissa da Conceição , Melo Jéssica Gomes Alcoforado de , Silva Gabriela Cavalcante da , Soares Diego Moura
Published: November 2022

Introdução: A alta ingestão de açucares, ácidos e de grãos de cereais processados, fez com que os índices de cáries e erosão dentárias aumentassem seus números. As balas, devido ao tempo que elas demoram a ser completamente dissolvidas na boca, podem influênciar no aparecimento da cárie. Além disso, algumas balas também possuem em suas formulações um potencial erosivo. Objetivo: Esse artigo tem como objetivo avaliar a ação erosiva e cariogênica das balas Halls, nos sabores morango, menta e extraforte em saliva artificial. Metodologia: Foram utilizadas balas Halls® nos sabores morango, menta e extraforte dissolvidas em saliva artificial. As amostras foram submetidas a testes de pH, acidez titulável e determinação de açucares redutores. Todos os experimentos foram realizados em triplicata para todos os sabores analizados. Os resultados foram submetidos à análise da variância (ANOVA) foi utilizado o teste de Tukey como pós-teste. Considerou-se um nível de significância de 5% (p <0,05) para todas as análises. Resultados: Na análise do pH o sabor extraforte apresentou a maior média, sendo significativamente maior quando comparado aos demias. Da mesma forma, a determinação de açúcares redutores mostrou que o sabor extraforte apresenta uma maior quantidade de sacarose significativamente maior quando comparado com o de morango. Na análise da acidez titulável o sabor morango apresentou médias estatisticamente maiores quando comparado com o sabor menta e extraforte. Conclusão: Todas as balas Halls® analizadas, quando diluídas em saliva artificial, podem apresentar potencial erosivo e ação cariogênica independente do sabor.