bluuebunny/crossref_metadata_2025 huggingface dataset:
Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population
Authors: Wu Xiaodong , Liu Zhijuan , Hao Doudou , Zhao Qin , Li Wanjing , Xie Maodi , Feng Xia , Liao Xia , Chen Siyuan , Wang Siyu , Zhou Chaohua , Long Wenchun , Zhong Yajun , Li Shen , Cao Ye , Wang Hong , Wang Aiping , Xu Yuehong , Huang Min , Liu Jiaxin , Zhong Rui , Wu Yunhong , He Zeng
Published: September 2023

Due to environmental hypoxia on the Tibetan Plateau, local residents often exhibit a compensative increase in hemoglobin concentration to maintain the body’s oxygen supply. However, increases in hemoglobin and hematocrit (Hct) pose a serious challenge to the quality of stored suspended red blood cells (SRBCs) prepared from the blood of high-hemoglobin populations, especially populations at high altitude with polycythemia in Tibet. To explore the difference in storage quality of SRBCs prepared from plateau residents with a high hemoglobin concentration, blood donors were recruited from Tibet (> 3600 m) and Chengdu (≈ 500 m) and divided into a high-altitude control (HAC) group, high-altitude polycythemia (HAPC) group and lowland control (LLC) group according to their hemoglobin concentration and altitude of residence. The extracellular acidification rate (ECAR), pyruvate kinase (PK) activity and band 3 tyrosine phosphorylation were analyzed on the day of blood collection. Then, whole-blood samples were processed into SRBCs, and storage quality parameters were analyzed aseptically on days 1, 14, 21 and 35 of storage. Overall, we found that tyrosine 21 phosphorylation activated glycolysis by releasing glycolytic enzymes from the cytosolic domain of band 3, thus increasing glucose consumption and lactate accumulation during storage, in the HAPC group. In addition, band 3 tyrosine phosphorylation impaired erythrocyte deformability, accompanied by the highest hemolysis rate in the HAPC group, during storage. We believe that these results will stimulate new ideas to further optimize current additive solutions for the high-hemoglobin population in Tibet and reveal new therapeutic targets for the treatment of HAPC populations.
B7H3-targeting chimeric antigen receptor modification enhances antitumor effect of Vγ9Vδ2 T cells in glioblastoma
Authors: Wang Yi , Ji Nan , Zhang Yang , Chu Junsheng , Pan Changcun , Zhang Peng , Ma Weiwei , Zhang Xueguang , Xi Jianzhong Jeff , Chen Mingze , Zhang Yonghui , Zhang Liwei , Sun Tao
Published: September 2023

Background

Glioblastoma (GBM) is a highly aggressive primary brain tumor with a poor prognosis. This study investigates the therapeutic potential of human Vγ9Vδ2 T cells in GBM treatment. The sensitivity of different glioma specimens to Vγ9Vδ2 T cell-mediated cytotoxicity is assessed using a patient-derived tumor cell clusters (PTCs) model.

Methods

The study evaluates the anti-tumor effect of Vγ9Vδ2 T cells in 26 glioma cases through the PTCs model. Protein expression of BTN2A1 and BTN3A1, along with gene expression related to lipid metabolism and glioma inflammatory response pathways, is analyzed in matched tumor tissue samples. Additionally, the study explores two strategies to re-sensitize tumors in the weak anti-tumor effect (WAT) group: utilizing a BTN3A1 agonistic antibody or employing bisphosphonates to inhibit farnesyl diphosphate synthase (FPPS). Furthermore, the study investigates the efficacy of genetically engineered Vγ9Vδ2 T cells expressing Car-B7H3 in targeting diverse GBM specimens.

Results

The results demonstrate that Vγ9Vδ2 T cells display a stronger anti-tumor effect (SAT) in six glioma cases, while showing a weaker effect (WAT) in twenty cases. The SAT group exhibits elevated protein expression of BTN2A1 and BTN3A1, accompanied by differential gene expression related to lipid metabolism and glioma inflammatory response pathways. Importantly, the study reveals that the WAT group GBM can enhance Vγ9Vδ2 T cell-mediated killing sensitivity by incorporating either a BTN3A1 agonistic antibody or bisphosphonates. Both approaches support TCR-BTN mediated tumor recognition, which is distinct from the conventional MHC-peptide recognition by αβ T cells. Furthermore, the study explores an alternative strategy by genetically engineering Vγ9Vδ2 T cells with Car-B7H3, and both non-engineered and Car-B7H3 Vγ9Vδ2 T cells demonstrate promising efficacy in vivo, underscoring the versatile potential of Vγ9Vδ2 T cells for GBM treatment.

Conclusions

Vγ9Vδ2 T cells demonstrate a robust anti-tumor effect in some glioma cases, while weaker in others. Elevated BTN2A1 and BTN3A1 expression correlates with improved response. WAT group tumors can be sensitized using a BTN3A1 agonistic antibody or bisphosphonates. Genetically engineered Vγ9Vδ2 T cells, i.e.,  Car-B7H3, show promising efficacy. These results together highlight the versatility of Vγ9Vδ2 T cells for GBM treatment.
A comparative study on the clinical efficacy of microplate trans-carpometacarpal joint fixation and non-trans-carpometacarpal joint fixation in treating fractures with dislocation or subluxation of the base of the fourth and fifth metacarpal bones
Authors: Zhao Liang
Published: September 2023

Background

This study aimed to compare the clinical efficacy of microplate trans-carpometacarpal joint fixation and non-trans-carpometacarpal joint fixation in treating fractures and dislocation or subluxation of the base of the fourth and fifth metacarpal bones.

Method

From 2015 to 2021, 100 cases of metacarpal basal fractures with dislocation or subluxation were randomly divided into the trans-carpometacarpal joint fixation group (group A) and non-trans-carpometacarpal joint fixation group (group B). Group A (n = 50) comprised 44 males and 6 females, with an average age of 28.8 ± 6.1 y and an Orthopedic Trauma Association (OTA) fracture classification of type B1 (n = 29) or C1 (n = 21). Group B (n = 50) comprised 45 males and 5 females, with an average age of 28.9 ± 5.7 y and an OTA fracture classification of type B1 (n = 28) or C1 (n = 22). All patients were complicated with dislocation or subluxation. The surgery time, fracture healing time, postoperative handgrip strength, and total active motion (TAM) scores of the ring and little fingers were recorded and compared between the two groups. The clinical efficacy of patients was evaluated using scoring methods such as DASH (disabilities of the arm, shoulder and hand), visual analogue scale (VAS), and Mayo at 3, 6, and 12 months after surgery.

Results

There was no significant difference in the general indexes, surgery time, or fracture healing time between the two groups (P > 0.05). There were no significant differences in handgrip strength and TAM scores of the ring and little fingers between the two groups at 3 and 12 months postoperatively (P > 0.05), but there were significant differences in these indexes 6 months postoperatively (P < 0.05). There were no significant differences in the DASH, VAS, and Mayo scores at 3 and 12 months postoperatively (P > 0.05), but there were significant differences between the two groups in the DASH and Mayo scores (P < 0.05) but not the VAS score (P > 0.05) 6 months postoperatively.

Conclusion

In the treatment of fourth and fifth metacarpal basal fractures with dislocation or subluxation, both microplate transarticular fixation and non-transarticular fixation could achieve fracture fixation and healing, and each method had advantages and disadvantages. The clinically appropriate fixation method should be selected according to the experience of the surgeon and the degree and type of fracture and dislocation.
Double-pivot proper digital artery perforator flap for fingertip reconstruction
Authors: Liu Benquan , Pan Ding , Gao Zhiyu , Duan Pengfei , Ou Qifeng
Published: September 2023

Background

Dorsal flap based on proper digital artery perforator has been commonly used in wound coverage of fingertip; yet a small diameter and short length poses a risk of pedicle kinking or occlusion. The present study aims to present our preliminary results of using a double-pivot perforator flap based on the end dorsal branch of proper digital artery to repair finger pulp defect.

Methods

We designed a double-pivot flap based on the end-dorsal perforator branch of proper digital artery, raised from the dorsal aspect of the middle phalanx, with inclusion of both the perforator and a section of the trunk of the artery. This modified procedure forms a pedicle with a larger diameter and length than traditional designs. Twelve patients (12 fingers) each with a soft-tissue defect of the fingertip were successfully treated and followed up in this retrospective study.

Results

All the flaps survived without showing any signs of necrosis; three cases presented with transient venous flow disorder, these self-resolving without requiring any additional treatment. At final follow-up (12–33 months, mean 20 months), mean static two-point discrimination on the flap was 7.0 mm (range, 6–9).

Conclusion

The double-pivot proper digital artery flap serves as a reliable option in fingertip reconstruction offering added benefits of having greater rotation flexibility, a lower risk of vessel kinking or occlusion, and good recovery of cutaneous sensation.
The Q/R editing site of AMPA receptor GluA2 subunit acts as an epigenetic switch regulating dendritic spines, neurodegeneration and cognitive deficits in Alzheimer’s disease
Authors: Wright Amanda L. , Konen Lyndsey M. , Mockett Bruce G. , Morris Gary P. , Singh Anurag , Burbano Lisseth Estefania , Milham Luke , Hoang Monica , Zinn Raphael , Chesworth Rose , Tan Richard P. , Royle Gordon A. , Clark Ian , Petrou Steven , Abraham Wickliffe C. , Vissel Bryce
Published: September 2023

Background

RNA editing at the Q/R site of GluA2 occurs with ~99% efficiency in the healthy brain, so that the majority of AMPARs contain GluA2(R) instead of the exonically encoded GluA2(Q). Reduced Q/R site editing increases AMPA receptor calcium permeability and leads to dendritic spine loss, neurodegeneration, seizures and learning impairments. Furthermore, GluA2 Q/R site editing is impaired in Alzheimer’s disease (AD), raising the possibility that unedited GluA2(Q)-containing AMPARs contribute to synapse loss and neurodegeneration in AD. If true, then inhibiting expression of unedited GluA2(Q), while maintaining expression of GluA2(R), may be a novel strategy of preventing synapse loss and neurodegeneration in AD.

Methods

We engineered mice with the ‘edited’ arginine codon (CGG) in place of the unedited glutamine codon (CAG) at position 607 of the Gria2 gene. We crossbred this line with the J20 mouse model of AD and conducted anatomical, electrophysiological and behavioural assays to determine the impact of eliminating unedited GluA2(Q) expression on AD-related phenotypes.

Results

Eliminating unedited GluA2(Q) expression in AD mice prevented dendritic spine loss and hippocampal CA1 neurodegeneration as well as improved working and reference memory in the radial arm maze. These phenotypes were improved independently of Aβ pathology and ongoing seizure susceptibility. Surprisingly, our data also revealed increased spine density in non-AD mice with exonically encoded GluA2(R) as compared to their wild-type littermates, suggesting an unexpected and previously unknown role for unedited GluA2(Q) in regulating dendritic spines.

Conclusion

The Q/R editing site of the AMPA receptor subunit GluA2 may act as an epigenetic switch that regulates dendritic spines, neurodegeneration and memory deficits in AD.
LncRNA like NMRK2 mRNA functions as a key molecular scaffold to enhance mitochondrial respiration of NONO-TFE3 rearranged renal cell carcinoma in an NAD+ kinase-independent manner
Authors: Chen Yi , Lu Yanwen , Yang Lei , Ma Wenliang , Dong Yuhan , Zhou Shuoming , Liu Ning , Gan Weidong , Li Dongmei
Published: September 2023

Background

NONO-TFE3 rearranged renal cell carcinoma (NONO-TFE3 rRCC) is one of a subtype of TFE3 rRCCs with high malignancy and poor prognosis. Compared with clear cell RCC, NONO-TFE3 rRCC shows a preference for mitochondrial respiration. We recently identified that the upregulation of nicotinamide ribokinase 2 (NMRK2) was associated with enhanced mitochondrial respiration and tumor progression in TFE3 rRCC.

Methods

A tumor-bearing mouse model was established to verify the pro-oncogenic effect of NMRK2 on NONO-TFE3 rRCC. Then the expression of NMRK2 RNA and protein was detected in cell lines and patient specimens. The NMRK2 transcripts were Sanger-sequenced and blasted at NCBI website. We constructed dCas13b-HA system to investigate the factors binding with NMRK2 RNA. We also used molecular experiments like RIP-seq, IP-MS, FISH and fluorescence techniques to explore the mechanisms that long non-coding RNA (lncRNA) like NMRK2 mRNA promoted the mitochondrial respiration of NONO-TFE3 rRCC. The efficacy of the combination of shRNA (NMRK2)-lentivirus and metformin on NONO-TFE3 rRCC was assessed by CCK-8 assay.

Results

In this study, we confirmed that NMRK2 showed transcriptional-translational conflict and functioned as lncRNA like mRNA in the NONO-TFE3 rRCC. Furthermore, we revealed the molecular mechanism that NONO-TFE3 fusion suppressed the translation of NMRK2 mRNA. Most importantly, three major pathways were shown to explain the facilitation effects of lncRNA like NMRK2 mRNA on the mitochondrial respiration of NONO-TFE3 rRCC in an NAD+ kinase-independent manner. Finally, the efficacy of combination of shRNA (NMRK2)-lentivirus and metformin on NONO-TFE3 rRCC was demonstrated to be superior than either agent alone.

Conclusions

Overall, our data comprehensively demonstrated the mechanisms for the enhanced mitochondrial respiration in NONO-TFE3 rRCC and proposed lncRNA like NMRK2 mRNA as a therapy target for NONO-TFE3 rRCC.

Graphical Abstract
Effectiveness of a treat-to-target strategy in patients with moderate to severely active rheumatoid arthritis treated with abatacept
Authors: Bessette Louis , Haraoui Boulos , Rampakakis Emmanouil , Dembowy Joanna , Trépanier Marc-Olivier , Pope Janet
Published: September 2023

Background

To compare a treat-to-target (T2T) approach and routine care (RC) in adults with active to severely active rheumatoid arthritis (RA) initiating subcutaneous abatacept.

Methods

A 12-month cluster-randomized trial in active RA patients treated with abatacept was conducted. Physicians were randomized to RC or T2T with a primary endpoint of achieving sustained Clinical Disease Activity Index (CDAI) low disease activity (LDA) at two consecutive assessments approximately 3 months apart. Additional outcomes included Simple Disease Activity Index (SDAI), Disease Activity Score 28-CRP (DAS28-CRP), Routine Assessment of Patient Index Data 3 (RAPID3), and the Health Assessment Questionnaire-Disability Index (HAQ-DI). Time to achieve therapeutic endpoints was assessed with survival analysis.

Results

Among the 284 enrolled patients, 130 were in the T2T group and 154 in RC. Primary endpoint was achieved by 36.9% and 40.3% of patients in T2T and RC groups, respectively. No significant between-group differences were observed in the odds of achieving secondary outcomes, except for a higher likelihood of CDAI LDA in the T2T group vs. RC (odds ratio [95% confidence interval]: 1.33 [1.03–1.71], p = 0.0263). Compared with RC, patients in the T2T group achieved SDAI remission significantly faster (Kaplan–Meier-estimated mean [standard error]: 14.0 [0.6] vs. 19.3 [0.8] months, p = 0.0428) with a trend toward faster achievement of CDAI LDA/remission, DAS28-CRP remission, and HAQ-DI minimum clinically important difference.

Conclusions

Patients managed per T2T and those under RC experienced significant improvements in RA disease activity at 12 months of abatacept treatment. T2T was associated with higher odds of CDAI LDA and a shorter time to achieving therapeutic endpoints.

Trial registration

Name of the registry: ClinicalTrials.gov.

Trial registrations: NCT03274141.

Date of registration: September 6, 2017.
Comparison of mechanical energy transfer during right-forward lunge between female amateur and professional badminton players
Authors: Safavi Soheila , Sheikhhoseini Rahman , Abdollahi Sajjad
Published: September 2023

Background

Regarding their skill levels, badminton players present different movement patterns during front and right lunging. The main objective of this study was to compare the mechanical energy transfers attributable to right-forward lunges between amateur and professional badminton players to study variations in mechanical efficiency at various skill levels.

Method

In this cross-sectional study, twenty female badminton players were recruited (Professional group n = 10 and Amateur group n = 10). The kinematics and kinetics of the lower extremities were recorded while performing right-forward lunges using Vicon motion capture and Kistler force plates. Mechanical energy expenditures (MEE) were extracted in eccentric transfer, concentric transfer, and no-transfer phases for the hip, knee, and ankle joints. At each joint, mechanical energy compensations (MEC) were also determined. Independent samples t-tests were used to analyze data at a significance level of α = 0.05.

Result

Regards to mechanical energy expenditures at the initial heel contact phase, the professional players demonstrated statistically significant more ankle no-transfer (p < 0.003), less knee concentric transfer (p < 0.026), more knee eccentric transfer (p < 0.001), and less hip no-transfer (p < 0.001). At the same time, the amateur athletes showed significantly more ankle eccentric transfer (p < 0.042) at maximal knee flexion angle time point. Analyzing mechanical energy compensation coefficients showed that the professional athletes had significantly less ankle concentric transfer (p < 0.001), more knee concentric transfer (p < 0.001), more knee eccentric transfer (p < 0.001), and more hip eccentric transfer (p < 0.001) at initial contact phase. While they found to have significantly more ankle eccentric transfer (p < 0.007), less knee concentric transfer (p < 0.001), less knee eccentric transfer (p < 0.001), more hip concentric transfer (p < 0.001), and more hip eccentric transfer (p < 0.001) at maximal knee flexion angle.

Conclusion

it is shown that the mechanical energy efficiency of the right-forward lunge is skill-related. It seems that altered lunge landing biomechanics may increase the risk of ankle and knee injuries and muscular damages in amateur athletes. It is recommended for amateur players to follow a injury prevention training program that promotes proper lunging technique.
Thermotolerant PGPR consortium B3P modulates physio-biochemical and molecular machinery for enhanced heat tolerance in maize during early vegetative growth
Authors: Ahmad Muhammad , Imtiaz Muhammad , Nawaz Muhammad Shoib , Mubeen Fathia , Sarwar Yasra , Hayat Mahnoor , Asif Muhammad , Naqvi Rubab Zahra , Ahmad Muhammad , Imran Asma
Published: September 2023

Background

Global warming and irregular changes in temperature are a serious threat to plant growth with a significant negative impact on yield. Global maize productivity has decreased significantly due to sudden temperature fluctuations and heat waves especially in the regions severely hit by climate change.

Results

The current study demonstrates the potential of beneficial bacteria for inducing heat tolerance in maize during early growth. Three Bacillus spp. AH-08, AH-67, SH-16, and one Pseudomonas spp. SH-29 showed the ability to grow and exhibited multiple plant-beneficial traits up to 45 ± 2 °C. At temperatures of 45 and 50 °C, Bacillus sp. SH-16 exhibited upregulation of two small heat shock proteins (HSP) of 15 and 30 kDa, while SH-16 and AH-67 showed upregulation of two large HSP of 65 and 100 kDa. Plant-inoculation with the consortium B3P (3 Bacillus + 1 Pseudomonas spp.) was carried out on six hybrid maize varieties pre-grown at 25 ± 2 ºC. Heat shock was applied to 10-day-old seedlings as: 3 h at 38ºC, 48 h recovery period, and then 48 h at 42ºC. The B3P treatment showed significant improvement in the overall plant growth (plant height, root & shoot fresh & dry weight, root and leaf area) with a higher level of CAT, POD, total chlorophyll, and carotenoids, while low concentration of MDA. A non-significant difference was observed in case of total cell protein and amino acids after B3P-treatment under stress. The expression of HSP1 and HSP18 in Malka and YH-5427 while HSP70 and HSP101 were higher in FH-1046 and Gohar as compared to non-inoculated treatment.

Conclusions

These findings indicate that heat-tolerant plant-growth promoting rhizobacteria (Ht-PGPR) exert versatile, multiphasic and differential response to improve plant growth and heat-tolerance in different maize varieties during seedling/ early vegetative growth. Subsequent research will be focused on the field evaluation of these PGPR to see the field and yield response of this consortium under natural temperature fluctuations in field.
Sex differences in obesity-induced renal lipid accumulation revealed by lipidomics: a role of adiponectin/AMPK axis
Authors: Juszczak Florian , Pierre Louise , Decarnoncle Morgane , Jadot Inès , Martin Blanche , Botton Olivia , Caron Nathalie , Dehairs Jonas , Swinnen Johannes V. , Declèves Anne-Emilie
Published: September 2023

Background

Sex differences have been observed in the development of obesity-related complications in patients, as well as in animal models. Accumulating evidence suggests that sex-dependent regulation of lipid metabolism contributes to sex-specific physiopathology. Lipid accumulation in the renal tissue has been shown to play a major role in the pathogenesis of obesity-induced kidney injury. Unlike in males, the physiopathology of the disease has been poorly described in females, particularly regarding the lipid metabolism adaptation.

Methods

Here, we compared the lipid profile changes in the kidneys of female and male mice fed a high-fat diet (HFD) or low-fat diet (LFD) by lipidomics and correlated them with pathophysiological changes.

Results

We showed that HFD-fed female mice were protected from insulin resistance and hepatic steatosis compared to males, despite similar body weight gains. Females were particularly protected from renal dysfunction, oxidative stress, and tubular lipid accumulation. Both HFD-fed male and female mice presented dyslipidemia, but lipidomic analysis highlighted differential renal lipid profiles. While both sexes presented similar neutral lipid accumulation with obesity, only males showed increased levels of ceramides and phospholipids. Remarkably, protection against renal lipotoxicity in females was associated with enhanced renal adiponectin and AMP-activated protein kinase (AMPK) signaling. Circulating adiponectin and its renal receptor levels were significantly lower in obese males, but were maintained in females. This observation correlated with the maintained basal AMPK activity in obese female mice compared to males.

Conclusions

Collectively, our findings suggest that female mice are protected from obesity-induced renal dysfunction and lipotoxicity associated with enhanced adiponectin and AMPK signaling compared to males.
Analysis and implementation of the DynDiff tool when comparing versions of ontology
Authors: Diaz Benavides Sara , Cardoso Silvio D. , Da Silveira Marcos , Pruski Cédric
Published: September 2023

Background

Ontologies play a key role in the management of medical knowledge because they have the properties to support a wide range of knowledge-intensive tasks. The dynamic nature of knowledge requires frequent changes to the ontologies to keep them up-to-date. The challenge is to understand and manage these changes and their impact on depending systems well in order to handle the growing volume of data annotated with ontologies and the limited documentation describing the changes.

Methods

We present a method to detect and characterize the changes occurring between different versions of an ontology together with an ontology of changes entitled DynDiffOnto, designed according to Semantic Web best practices and FAIR principles. We further describe the implementation of the method and the evaluation of the tool with different ontologies from the biomedical domain (i.e. ICD9-CM, MeSH, NCIt, SNOMEDCT, GO, IOBC and CIDO), showing its performance in terms of time execution and capacity to classify ontological changes, compared with other state-of-the-art approaches.

Results

The experiments show a top-level performance of DynDiff for large ontologies and a good performance for smaller ones, with respect to execution time and capability to identify complex changes. In this paper, we further highlight the impact of ontology matchers on the diff computation and the possibility to parameterize the matcher in DynDiff, enabling the possibility of benefits from state-of-the-art matchers.

Conclusion

DynDiff is an efficient tool to compute differences between ontology versions and classify these differences according to DynDiffOnto concepts. This work also contributes to a better understanding of ontological changes through DynDiffOnto, which was designed to express the semantics of the changes between versions of an ontology and can be used to document the evolution of an ontology.
A new giant nautilid species from the Middle Jurassic of Luxembourg and Southwest Germany
Authors: Weis Robert , Schweigert Günter , Wittische Julian
Published: September 2023

In comparison to other cephalopods such as ammonites and belemnites, nautilid shells are relatively rare fossils in Jurassic marine deposits and knowledge of their taxonomy is therefore still patchy. We describe herein a new species of Cenoceras, C. rumelangense, from the early Bajocian Humphriesianum Zone of Luxembourg and Southwest Germany. In Luxembourg, the type material occurs in the ‘Marnes sableuses d’Audun-le-Tiche’ unit, which yields other large-sized cephalopods, such as the largest known belemnite genus, Megateuthis. The new species reaches a remarkable size, with diameters up to 610 mm. Thus, it is amongst the largest known post-Triassic nautilids worldwide, together with Paracenoceras giganteum and Paracenoceras ingens from the Upper Jurassic. Additionally, we discuss some aspects of the taphonomy of these large shells deposited in a shallow marine setting.

ZooBank LSID: urn:lsid:zoobank.org:pub:376E5DF8-653D-429C-8AD6-835D325D2912
Sensitivity of an AI method for [18F]FDG PET/CT outcome prediction of diffuse large B-cell lymphoma patients to image reconstruction protocols
Authors: Ferrández Maria C. , Golla Sandeep S. V. , Eertink Jakoba J. , de Vries Bart M. , Wiegers Sanne E. , Zwezerijnen Gerben J. C. , Pieplenbosch Simone , Schilder Louise , Heymans Martijn W. , Zijlstra Josée M. , Boellaard Ronald
Published: September 2023

Background

Convolutional neural networks (CNNs), applied to baseline [18F]-FDG PET/CT maximum intensity projections (MIPs), show potential for treatment outcome prediction in diffuse large B-cell lymphoma (DLBCL). The aim of this study is to investigate the robustness of CNN predictions to different image reconstruction protocols. Baseline [18F]FDG PET/CT scans were collected from 20 DLBCL patients. EARL1, EARL2 and high-resolution (HR) protocols were applied per scan, generating three images with different image qualities. Image-based transformation was applied by blurring EARL2 and HR images to generate EARL1 compliant images using a Gaussian filter of 5 and 7 mm, respectively. MIPs were generated for each of the reconstructions, before and after image transformation. An in-house developed CNN predicted the probability of tumor progression within 2 years for each MIP. The difference in probabilities per patient was then calculated between both EARL2 and HR with respect to EARL1 (delta probabilities or ΔP). We compared these to the probabilities obtained after aligning the data with ComBat using the difference in median and interquartile range (IQR).

Results

CNN probabilities were found to be sensitive to different reconstruction protocols (EARL2 ΔP: median = 0.09, interquartile range (IQR) = [0.06, 0.10] and HR ΔP: median = 0.1, IQR = [0.08, 0.16]). Moreover, higher resolution images (EARL2 and HR) led to higher probability values. After image-based and ComBat transformation, an improved agreement of CNN probabilities among reconstructions was found for all patients. This agreement was slightly better after image-based transformation (transformed EARL2 ΔP: median = 0.022, IQR = [0.01, 0.02] and transformed HR ΔP: median = 0.029, IQR = [0.01, 0.03]).

Conclusion

Our CNN-based outcome predictions are affected by the applied reconstruction protocols, yet in a predictable manner. Image-based harmonization is a suitable approach to harmonize CNN predictions across image reconstruction protocols.
Predicting mammographic density with linear ultrasound transducers
Authors: Behrens Annika , Fasching Peter A. , Schwenke Eva , Gass Paul , Häberle Lothar , Heindl Felix , Heusinger Katharina , Lotz Laura , Lubrich Hannah , Preuß Caroline , Schneider Michael O. , Schulz-Wendtland Rüdiger , Stumpfe Florian M. , Uder Michael , Wunderle Marius , Zahn Anna L. , Hack Carolin C. , Beckmann Matthias W. , Emons Julius
Published: September 2023

Background

High mammographic density (MD) is a risk factor for the development of breast cancer (BC). Changes in MD are influenced by multiple factors such as age, BMI, number of full-term pregnancies and lactating periods. To learn more about MD, it is important to establish non-radiation-based, alternative examination methods to mammography such as ultrasound assessments.

Methods

We analyzed data from 168 patients who underwent standard-of-care mammography and performed additional ultrasound assessment of the breast using a high-frequency (12 MHz) linear probe of the VOLUSON® 730 Expert system (GE Medical Systems Kretztechnik GmbH & Co OHG, Austria). Gray level bins were calculated from ultrasound images to characterize mammographic density. Percentage mammographic density (PMD) was predicted by gray level bins using various regression models.

Results

Gray level bins and PMD correlated to a certain extent. Spearman’s ρ ranged from − 0.18 to 0.32. The random forest model turned out to be the most accurate prediction model (cross-validated R2, 0.255). Overall, ultrasound images from the VOLUSON® 730 Expert device in this study showed limited predictive power for PMD when correlated with the corresponding mammograms.

Conclusions

In our present work, no reliable prediction of PMD using ultrasound imaging could be observed. As previous studies showed a reasonable correlation, predictive power seems to be highly dependent on the device used. Identifying feasible non-radiation imaging methods of the breast and their predictive power remains an important topic and warrants further evaluation.

Trial registration 325-19 B (Ethics Committee of the medical faculty at Friedrich Alexander University of Erlangen-Nuremberg, Erlangen, Germany).
The critical role of platelet in cancer progression and metastasis
Authors: Zhou Lin , Zhang Zhe , Tian Yizhou , Li Zefei , Liu Zhongliang , Zhu Sibo
Published: September 2023

Platelets play a crucial role in cancer blood metastasis. Various cancer-related factors such as Toll-like receptors (TLRs), adenosine diphosphate (ADP) or extracellular matrix (ECM) can activate these small particles that function in hemostasis and thrombosis. Moreover, platelets induce Epithelial Mesenchymal Transition (EMT) to promote cancer progression and invasiveness. The activated platelets protect circulating tumor cells from immune surveillance and anoikis. They also mediate tumor cell arrest, extravasation and angiogenesis in distant organs through direct or indirect modulation, creating a metastatic microenvironment. This review summarizes the recent advances and progress of mechanisms in platelet activation and its interaction with cancer cells in metastasis.
Serum neurofilament light chain in COVID-19 and the influence of renal function
Authors: Körtvelyessy Peter , Diekämper Elena , Ruprecht Klemens , Endres Matthias , Stubbemann Paula , Kurth Florian , Graw Jan Adriaan , Menk Mario , Kuhle Jens , Wohlrab Felix
Published: September 2023

COVID-19 is associated with various neurological symptoms. Serum neurofilament light chain (sNfL) is a robust marker for neuroaxonal injury. Recent studies have shown that elevated levels of sNfL are associated with unfavorable outcome in COVID-19 patients. However, neuroaxonal injury is rare in COVID-19, and renal dysfunction and hypoxia, both of which are known in severe COVID-19, can also increase sNfL levels. Thus, the meaning and mechanisms of sNfL elevation in COVID-19 patients remain unclear. We evaluated sNfL levels in 48 patients with COVID-19 (mean age = 63 years) and correlated them to clinical outcome, the form of oxygen therapy, and creatinine. Levels of sNfL were age adjusted and compared with normal values and z-scores. COVID-19 patients treated with nasal cannula had normal sNfL levels (mean sNfL = 19.6 pg/ml) as well as patients with high-flow treatment (mean sNfL = 40.8 pg/ml). Serum NfL levels were statistically significantly higher in COVID-19 patients treated with mechanical ventilation on intensive care unit (ICU) (mean sNfL = 195.7 pg/ml, p < 0.01). There was a strong correlation between sNfL elevation and unfavorable outcome in COVID-19 patients (p < 0.01). However, serum creatinine levels correlated directly and similarly with sNfL elevation and with unfavorable outcome in COVID-19 patients (p < 0.01). Additionally, multivariate analysis for serum creatinine and sNfL showed that both variables are jointly associated with clinical outcomes. Our results identify renal dysfunction as an important possible confounder for sNfL elevation in COVID-19. Thus, serum creatinine and renal dysfunction should be strongly considered in studies evaluating sNfL as a biomarker in COVID-19.
Cross-modality representation learning from transformer for hashtag prediction
Authors: Khalil Mian Muhammad Yasir , Wang Qingxian , Chen Bo , Wang Weidong
Published: September 2023

Hashtags are the keywords that describe the theme of social media content and have become very popular in influence marketing and trending topics. In recent years, hashtag prediction has become a hot topic in AI research to help users with automatic hashtag recommendations by capturing the theme of the post. Most of the previous work mainly focused only on textual information, but many microblog posts contain not only text but also the corresponding images. This work explores both image-text features of the microblog post. Inspired by the self-attention mechanism of the transformer in natural language processing, the visual-linguistics pre-train model with transfer learning also outperforms many downstream tasks that require image and text inputs. However, most of the existing models for multimodal hashtag recommendation are based on the traditional co-attention mechanism. This paper investigates the cross-modality transformer LXMERT for multimodal hashtag prediction for developing LXMERT4Hashtag, a cross-modality representation learning transformer model for hashtag prediction. It is a large-scale transformer model that consists of three encoders: a language encoder, an object encoder, and a cross-modality encoder. We evaluate the presented approach on dataset InstaNY100K. Experimental results show that our model is competitive and achieves impressive results, including precision of 50.5% vs 46.12%, recall of 44.02% vs 38.93%, and F1-score of 47.04% vs 42.22% compared to the existing state-of-the-art baseline model.
Urinary creatinine concentration and urine color as indicators of specimen validity test
Authors: Park Jaehyeong , Kwon Nam Hee , Kim Seon Yeong , Ko Beom Jun , Kim Jin Young
Published: September 2023

In this study, the concentration of urinary creatinine (Cr) and urine color were analyzed, and a correlation obtained, to objectively verify normal urine samples prior to forensic drug testing. Cr was analyzed via a colorimetric method based on the Jaffé reaction using a Cobas C-311 analyzer (Roche SA, Basel, Switzerland; Hitachi, Tokyo, Japan). The Cr concentration for urine specimen validity testing was measured to screen urine samples submitted after dilution or upon the addition of a foreign substance that interferes with drug testing. Urine samples containing < 20 mg/dL of Cr were classified as abnormal. The Korea Standard Color Analysis program was used for urine color analysis and correlations with Cr concentration analyzed. The color and Cr concentration of 271 urine samples were analyzed according to age and sex. The mean ± standard deviation of Cr concentration in males and females was 136.4 ± 66.2 mg/dL and 109.5 ± 71.1 mg/dL, respectively, with a statistically insignificant difference (p = 0.4554). Furthermore, the participants were categorized into young (19–34 years), middle-aged (35–49 years), and elderly (≥ 50 years) groups to compare Cr concentration; however, no significant difference was found (p = 0.2143–0.983). Strong variable correlations were identified between Cr and the characteristics of urine color. Despite various factors such as water consumption, health problems, and vitamins, urine color was found applicable for urine specimen validity testing. Future plans include the development of a smartphone camera application for use in urine color analysis to identify abnormal urine samples.
Effect of Tai Chi on Young Adults with Subthreshold Depression via a Stress–Reward Complex: A Randomized Controlled Trial
Authors: Wu Jingsong , Song Jian , He Youze , Li Zhaoying , Deng Haiyin , Huang Zhenming , Xie Xiaoting , Wong Nichol M. L. , Tao Jing , Lee Tatia M. C. , Chan Chetwyn C. H.
Published: September 2023

Background

Subthreshold depression is a highly prevalent mood disorder in young adults. Mind–body exercises, such as Tai Chi, have been adopted as interventions for clinical depressive symptoms. However, the possible effect and underlying mechanism of Tai Chi on subthreshold depression of young individuals remain unclear. This randomized controlled study aimed to evaluate the effects of Tai Chi training and tested the combined stress and reward circuitry model for subthreshold depression.

Results

A total of 103 participants completed this trial, with 49 in the 12-week 24-style Tai Chi group and 54 participants in control group. Our results showed significantly lower scores on depressive symptoms (P = 0.002) and anxiety symptoms (P = 0.009) and higher scores on quality of life (P = 0.002) after Tai Chi training. There were significant reductions in salivary cortisol levels (P = 0.007) and putamen gray matter volume (P < 0.001) in the Tai Chi group. The changes in cortisol levels and putamen gray matter volume had direct (bootstrapping confidence interval [− 0.91, − 0.11]) and indirect effects (bootstrapping confidence interval [− 0.65, − 0.19]) on the changes induced by Tai Chi training on depressive symptoms, respectively.

Conclusion

The stress–reward complex results indicated an interaction between lowering stress levels and increasing reward circuitry activity associated with the alleviation of depressive symptoms among participants. The 12-week Tai Chi training was effective in improving the symptoms and quality of life of young adults with subthreshold depression.

Trial Registration Chinese Registry of Clinical Trials (Registration Number: ChiCTR1900028289, Registered December 12, 2019).
Dietary total antioxidant capacity is associated with lower disease severity and inflammatory and oxidative stress biomarkers in patients with knee osteoarthritis
Authors: Amirkhizi Farshad , Hamedi-Shahraki Soudabeh , Rahimlou Mehran
Published: September 2023

Background

This study was designed to evaluate the association between dietary total antioxidant capacity and clinical and biochemical variables in patients with osteoarthritis.

Methods

This cross-sectional study was conducted among 160 patients with mild-to-moderate knee osteoarthritis. The Likert version of the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC Index) was used to assess the severity of clinical symptoms in patients with knee osteoarthritis. The secondary outcomes included inflammatory and oxidative stress biomarkers. The participants' usual diets were assessed using a food frequency questionnaire (FFQ), and the dietary total antioxidant capacity (TAC) was calculated based on the ferric reducing antioxidant power method. Additionally, clinical and biochemical variables were evaluated using standard methods.

Results

The mean age of the participants was 57.2 ± 8.1 years, and 55.6% of them were females. The dietary TAC scores in this study ranged from 3.67 to 24.72, with a mean of 12.05 ± 5.3. We found a significant inverse trend between the dietary TAC score and the total Western Ontario and McMaster Universities Osteoarthritis (WOMAC) score (P = 0.001), as well as the WOMAC stiffness (P = 0.008) and WOMAC physical function scores (P = 0.001). Furthermore, dietary TAC was inversely associated with serum concentrations of interleukin-6 (IL-6) (β = − 0.18, P = 0.020), tumor necrosis factor-α (TNF-α) (β = − 0.67, P < 0.001), matrix metalloproteinase-1 (MMP-1) (β = − 0.33, P < 0.001), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (β = − 0.22, P = 0.005) levels.

Conclusion

The results of this study demonstrate an inverse association between dietary total antioxidant capacity and clinical and biochemical variables in patients with osteoarthritis.
Pericardial effusions and cardiac tamponade in hospitalized systemic sclerosis patients: analysis of the national inpatient sample
Authors: Basyal Bikash , Ullah Waqas , Derk Chris T.
Published: September 2023

Introduction

Clinically significant pericardial effusions and cardiac tamponade in systemic sclerosis (SSc) patients is uncommon and the factors that contribute to progression of pericardial involvement in SSc patients have not been well established.

Methods

A review of the national inpatient sample database was performed looking SSc related hospitalizations between 2002 and 2019. Data was collected on patients with pericardial effusions and cardiac tamponade and analyzed to identify and describe patient characteristics and comorbidities.

Results

Out of a total of 523,410 SSc hospitalizations, with an overall inpatient mortality rate of 4.7% (24,764 patients), pericardial effusion was identified in 3.1% of all hospitalizations (16,141 patients) out of which 0.2% (838 patients) had a diagnosis of cardiac tamponade. Patients with pericardial effusion were significantly more likely to have pulmonary circulatory disease (p = < 0.0001), congestive heart failure (p = < 0.0001) end stage renal disease (p = < 0.0001), diabetes (p = 0.015), and hypothyroidism (p = 0.025). Patients with cardiac tamponade were significantly more likely to have a history of coronary artery bypass graft surgery (p = 0.001) or atrial fibrillation (p = < 0.0001). Hospitalized patients with cardiac tamponade had a significantly increased mortality rate of 17.7% compared to 8.8% in patients with pericardial effusions without a tamponade physiology, with an odds ratio of 2.3 (1.97–2.86), p = < 0.0001.

Conclusion

Pericardial effusion and tamponade are associated with increased morbidity and mortality in SSc patients. Further studies are required to explore the role of patient comorbidities and characteristics in development into pericardial effusions or tamponade.
Frequency-dependent velocities and attenuations in fluid-saturated rocks: Fractures aligned in isotropic medium with random pore shape
Authors: He Lingyi , Di Bangrang , Ding Pinbo , Wei Jianxin
Published: September 2023

The modeling of seismic wave velocity and attenuation is crucial for the interpretation of seismic data. In fluid-saturated rocks, the presence of fractures causes dispersion and attenuation of seismic waves due to fluid flow between cracks and pores. Meanwhile, the parallel distribution of cracks is an important reason for the anisotropy of rocks. Combined with poroelasticity theory, we extend Chapman’s model to media with penny-shaped fractures and an isotropic porous matrix, for which no restrictions are placed on the pore shape of the matrix. We conduct simulations at different frequencies to indicate the predicted anisotropy of velocity and attenuation. The variation of velocities and attenuations with orientation angle is consistent with Chapman’s model. Because a typical porous matrix is used in the new model, predicted velocities are lower than those under assumption of spherical matrix pores. Then, we analyze the effect of pore compressibility of the matrix on the velocity and attenuation. We also conduct discussions about the effect of different porosities on velocity and attenuation. The analysis indicates that the predictions of the new model are more closely related to the modulus of the porous matrix than Chapman’s model. The strength of fluid flow in the new model also will change with the matrix pores. The comparison of the new model and Chapman’s model with experimental data indicates that the new model is in better agreement with the experimental results.
Survivorship Care in Middle-Income Countries: A Guideline Development for Colombia Using Breast Cancer as a Model
Authors: Peñaloza Maylin , Sánchez Oswaldo , García María A. , Murillo Raúl
Published: September 2023

Cancer survival has significantly increased during the past few decades, making survivorship care a key element of cancer control and posing several challenges for long-term care in low- and middle-income countries (LMIC). Most survivorship care guidelines emphasize the potential role of primary care physicians and the need for comprehensive care, with a preference for patient-centered over disease-centered approaches. However, guidelines developed in high-income countries are not always suitable for LMIC, where a shortage of oncology workforce, deficient training in primary care, and low access to comprehensive centers frequently induce undertreatment and a lack of follow-up. Despite universal health insurance coverage, Colombia has fragmented cancer care with deficient survivorship care, given its focus on relapse surveillance without integration of supportive care and comorbidity management, in addition to unequal access for low-income populations and distant regions. Using the breast cancer framework, we describe the development of a guideline for survivorship care on the basis of a risk approach and the proper integration of oncology specialists and family physicians. We used a three-phase process to develop recommendations for disease control (disease-centered review), interventions aimed at improving patients' quality of life (patient-centered review), and care delivery (delivery model review). We deem our proposal suitable for middle-income countries, which represents an input for more standardized survivorship care in these settings.
Implementing Nanovesicles for Boosting the Skin Permeation of Non-steroidal Anti-inflammatory Drugs
Authors: Abdelbari Manar Adel , Elshafeey Ahmed Hassen , Abdelbary Aly Ahmed , Mosallam Shaimaa
Published: September 2023

The consumption of non-steroidal anti-inflammatory drugs (NSAIDs) have increased lately around the world, as they are considered essential and popular drugs for effective reduction of pain and inflammation. They have analgesic, antipyretic, and anti-inflammatory activities; also, it was reported recently that they protect against various critical disorders like heart attacks and cancer. However, oral use of NSAIDs may cause several pulmonary, gastrointestinal, hepatic, cardiovascular, cerebral, and renal complications. Therefore, topical NSAIDs were recommended as a substitute to oral NSAIDs for the treatment of inflammation and pain. Still, the skin permeation of NSAIDs is considered a challenge, as the skin have an effective barrier function. Therefore, this review investigates various advanced vesicular nanocarriers and their applications through the skin, to augment the topical delivery of NSAIDs through stratum corneum over the conventional systems, enhance their effectiveness, and reduce the unwanted side effects. These innovative systems can manage bioavailability, solubility, stability, safety, and efficacy issues present in conventional systems.