The Influence of Fluid Intake Behavior on Cognition and Mood among College Students in Baoding, China
Authors: He Hairong , Zhang Jian Fen , Zhang Na , Du Songming , Liu Shufang , Ma Guansheng 
Published: March 2021

<b><i>Introduction:</i></b> Water is a critical nutrient, and it is important for the maintenance of the physiological function of the human body [<xref ref-type="bibr" rid="ref1">1</xref>-<xref ref-type="bibr" rid="ref3">3</xref>]. In addition to fluid amounts, fluid intake frequency is also important for hydration status [<xref ref-type="bibr" rid="ref4">4</xref>, <xref ref-type="bibr" rid="ref5">5</xref>]. At present, only few guidelines mention fluid intake behavior that recommend drinking water frequently while in small quantities, however, there is no scientific evidence to support it. Therefore, it is necessary to explore the appropriate fluid intake behavior. <b><i>Objective:</i></b> The objective of this study is to evaluate the influence of different fluid intake behavior on cognition and mood, to provide scientific basis for proposing the appropriate fluid intake behavior. <b><i>Methods:</i></b> A double-blinded randomized controlled trial was designed and implemented among college students aged 18–23 years in Baoding, China. Subjects were randomly assigned into each of 3 groups using a random number generated by computer software: the subjects consuming plain water 200 mL/2 h, that is, 1,600 mL during whole day (group 1), 100 mL/2 h, that is, 800 mL during whole day (group 2), and 110 mL/1 h, that is, 1,650 mL during whole day (group 3), respectively. Subjects were asked to fast from 11:00 p.m., without consuming any foods or drinks the day before the intervention. From 8:00 a.m. to 10:00 p.m. of the first study day, subjects consumed water according to the instructions and repeated it from 8:00 a.m. to 4:00 p.m. of the second study day. Cognition, mood, and urine osmolality were collected twice at 10:00 a.m. (time 1) and 4:00 p.m. (time 2) of the second study day (shown in Fig. <xref ref-type="fig" rid="f01">1</xref>). <b><i>Results:</i></b> A total of 92 subjects (46 males, 46 females) completed this study. It was found that the increasing fluid intake amounts lead to an increase in urine output and a decrease in urine osmolality (<i>p</i> &#x3c; 0.05). Use the mixed models to compare measurements for groups 1 and 2, which showed that when compared with those drinking 800 mL per day, people who drank 1,600 mL per day scored higher in vigor (11.8 vs. 9.1, <i>p</i> &#x3c; 0.05) and portrait memory test (22.6 vs. 20.8, <i>p</i> &#x3c; 0.05) but lower in total mood disturbance (90.8 vs. 97.8, <i>p</i> &#x3c; 0.05). By comparing groups 1 and 3, the results indicated that compared with drinking 8 times per day, people who drank 15 times per day scored lower in portrait memory test (21.8 vs. 22.6, <i>p</i> &#x3c; 0.05) and hunger (3.3 vs. 3.6, <i>p</i> &#x3c; 0.05). <b><i>Conclusions:</i></b> Reasonable fluid intake behavior may be beneficial to improve the cognition and mood of college students. The fluid intake behavior, which is consuming water 200 mL each time and 8 times per day, is recommended. More studies are needed to advise people to have health-beneficial fluid intake behavior.