bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid State of Health Estimation Strategy for Retired Batteries Based on Resting Voltage Curves
Authors: Huang Haihong , Liu Xin , Chang Wenjing , Wang Yuhang
Published: February 2025

Retired batteries are approaching the recycling peak, and their secondary utilization can prevent resource waste and environmental pollution from battery retirement. Evaluating the state of health (SOH) of retired batteries is crucial for secondary use. However, estimating the SOH of retired batteries is time-consuming and energy-intensive. To address the problem, this paper proposes a rapid estimation strategy based on resting voltage curves. After discharging retired batteries to the same voltage, variations in the remaining state of charge (SOC) exist among batteries with different SOHs. These SOC differences lead to distinct trends in the resting voltage curves for varying SOH batteries. Our approach analyzes health features from these resting voltage discrepancies, ultimately achieving a fast estimation of retired batteries’ SOH. Additionally, during the data collection of datasets, some batteries may form outliers due to measurement errors. This paper analyzes the impact of outlier quantity on the accuracy of regression models for SOH estimation and proposes using the DBSCAN clustering algorithm to identify and mitigate the influence of outliers, eventually enhancing the precision of SOH estimation.
Automating Speech Audiometry in Quiet and in Noise Using a Deep Neural Network
Authors: Jean Hadrien , Wallaert Nicolas , Dreumont Antoine , Creff Gwenaelle , Godey Benoit , Paraouty Nihaad
Published: February 2025

In addition to pure-tone audiometry tests and electrophysiological tests, a comprehensive hearing evaluation includes assessing a subject’s ability to understand speech in quiet and in noise. In fact, speech audiometry tests are commonly used in clinical practice; however, they are time-consuming as they require manual scoring by a hearing professional. To address this issue, we developed an automated speech recognition (ASR) system for scoring subject responses at the phonetic level. The ASR was built using a deep neural network and trained with pre-recorded French speech materials: Lafon’s cochlear lists and Dodelé logatoms. Next, we tested the performance and reliability of the ASR in clinical settings with both normal-hearing and hearing-impaired listeners. Our findings indicate that the ASR’s performance is statistically similar to manual scoring by expert hearing professionals, both in quiet and in noisy conditions. Moreover, the test–retest reliability of the automated scoring closely matches that of manual scoring. Together, our results validate the use of this deep neural network in both clinical and research contexts for conducting speech audiometry tests in quiet and in noise.
Periplaneta americana Extract Protects Glutamate-Induced Nerve Cell Damage by Inhibiting N-Methyl-D-Aspartate Receptor Activation
Authors: Zhou Yongfang , Yang Xin , Hong Canhui , Guan Tangfei , Xiao Peiyun , Yang Yongshou , Zhang Chenggui , He Zhengchun
Published: February 2025

Neurocytotoxicity elicited by N-methyl-D-aspartate receptors (NMDAR) is a substantial contributor to neurodegenerative diseases. Our current study expands on the previous findings in which Periplaneta americana (L.) extract (PAS840) was shown to protect PC12 cells from hydrogen peroxide-induced injury. In this investigation, we performed LC-MS/MS and peptidomics analyses on the constituents of PAS840. Considering Alzheimer’s disease (AD) as the primary focus, we utilized network pharmacology and molecular-docking techniques to predict PAS840’s influence on AD targets. We established a glutamate (Glu)-induced PC12 cell injury model to conduct a comprehensive examination of PAS840’s effects on pivotal cellular parameters, including intracellular Ca2+ levels, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, reactive oxygen species (ROS) levels, cell apoptosis rate, mitochondrial membrane potential (MMP) levels, and the expression of key proteins such as NMDAR1, cytochrome c (Cytc), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). The results suggest that PAS840 manifests multi-target actions, robustly attenuating NMDAR activity. It efficaciously suppresses excessive NMDAR1 activation, restricts Ca2+ influx, alleviates oxidative stress, and mitigates inflammation, thereby ameliorating neuronal cell damage. Consequently, it establishes a solid scientific foundation for further exploration of PAS840’s potential in addressing neurological diseases.
Modulation of Wnt/Beta-Catenin Pathway by Major Dietary Phytochemicals Against Breast Cancer Development
Authors: Lieb Noah , Tran Annalisa , Torres Martha , Bommareddy Ajay
Published: February 2025

Breast cancer is one of the most commonly diagnosed cancers and is the second leading cause of cancer-related deaths among women in the United States. Despite a decrease in mortality associated with breast cancer, there has been a steady increase in its incidence. Development of the mammary gland is normally regulated by such pathways including Wnt, Hedgehog, estrogen and Notch signaling. However, the deregulation of these pathways gives rise to tumor development, and upregulated Wnt activity along with high levels of beta-catenin is correlated with poor prognosis of breast cancer. In addition, beta-catenin-dependent Wnt signaling is enriched in triple-negative breast cancers and is associated with reduced overall survival in breast cancer patients. Various studies have investigated the ability of naturally occurring plant-based agents to reduce incidence and morbidity of breast cancer by regulating critical cell survival pathways to reverse or inhibit the occurrence of clinical disease. The present review focuses on summarizing the role of commonly consumed dietary phytochemicals and their role in regulating Wnt/β-catenin pathway against the development of breast cancer.
MicroRNAs Associated with Parenchymal Hematoma After Endovascular Mechanical Reperfusion for Acute Ischemic Stroke in Rats
Authors: Zhuang Jin-Kun , Huang Zhong-Run , Qin Wang , Li Chang-Luo , Li Qi , Xiang Chun , Tuo Yong-Hua , Liu Zhong , Chen Qian-Yu , Shi Zhong-Song
Published: February 2025

Background/Objectives: Hemorrhagic transformation after endovascular thrombectomy predicts poor outcomes in acute ischemic stroke with large-vessel occlusion. The roles of microRNAs (miRNAs) in the pathogenesis of parenchymal hematoma (PH) after endovascular thrombectomy still remain unclear. This study aimed to investigate the miRNA and mRNA regulatory network associated with PH after mechanical reperfusion in an animal stroke model and an oxygen–glucose deprivation/reoxygenation (OGD/R) model. Methods: Twenty-five miRNAs were assessed in a mechanical reperfusion-induced hemorrhage transformation model in rats under hyperglycemic conditions receiving 5 h middle cerebral artery occlusion. The differentially expressed miRNAs associated with PH were assessed in a neuron, astrocyte, microglia, brain microvascular endothelial cell (BMEC), and pericyte model of OGD/R. The predicted target genes of the differentially expressed miRNAs were further assessed in the animal model. The miRNA-mRNA regulatory network of PH was established. Results: Thirteen down-regulated miRNAs (miRNA-29a-5p, miRNA-29c-3p, miRNA-126a-5p, miRNA-132-3p, miRNA-136-3p, miRNA-142-3p, miRNA-153-5p, miRNA-218a-5p, miRNA-219a-2-3p, miRNA-369-5p, miRNA-376a-5p, miRNA-376b-5p, and miRNA-383-5p) and one up-regulated miRNA (miRNA-195-3p) were found in the rat peri-infarct with PH after mechanical reperfusion. Of these 14 PH-related miRNAs, 10 were significantly differentially expressed in at least two of the five neuron, astrocyte, microglia, BMEC, and pericyte models after OGD/R, consistent with the animal stroke model results. Thirty-one predicted hub target genes were significantly differentially expressed in the rat peri-infarct with PH after mechanical reperfusion. Forty-nine miRNA-mRNA regulatory axes of PH were revealed, and they were related to the mechanisms of inflammation, immunity, oxidative stress, and apoptosis. Conclusions: Fourteen miRNAs were associated with PH after mechanical reperfusion in the rat stroke and the OGD/R models. Simultaneously differentially expressed miRNAs and related genes in several cells of the neurovascular unit may serve as valuable targets for PH after endovascular thrombectomy in acute ischemic stroke.
Pharmacological Modulation of Mutant TP53 with Oncotargets Against Esophageal Cancer and Therapy Resistance
Authors: Lin Pei-I , Lee Yu-Cheng , Chen I-Hung , Chung Hsien-Hui
Published: February 2025

The prevalence and deaths from esophageal cancer (EC) have recently increased. Although therapeutic strategies depend on the EC stage and recurrence, such as surgical intervention, chemotherapy, radiation therapy, chemoradiation therapy, targeted therapy, and immunotherapy, a more effective and novel treatment for EC is still required. This review briefly describes and summarizes some insightful oncotargets involved in the metabolic modulation of EC, including (1) cancer stem cells (CSCs) for EC progression, poor prognosis, tumor recurrence, and therapy resistance; (2) retinoic acid receptors (RARs) for esophageal carcinogenesis and regeneration; (3) phosphofructokinase (PFK) for EC-reprogrammed glycolysis; (4) lactate dehydrogenase (LDH) as an EC peripheral blood biomarker; and (5) hypoxia-inducible factor-1 alpha (HIF-1α) for the tumor microenvironment under hypoxic conditions. Moreover, the aforementioned oncotargets can be modulated by mutant TP53 and have their own features in the carcinogenesis, differentiation, proliferation, and metastasis of EC. Thus, the clarification of pharmacological mechanisms regarding the interaction between mutant TP53 and the abovementioned oncotargets could provide precise and perspective opinions for minimizing prediction errors, reducing therapy resistance, and developing novel drugs against EC.
Animal Models for Studying Developmental Origins of Cardiovascular–Kidney–Metabolic Syndrome
Authors: Tain You-Lin , Lin Ying-Jui , Hsu Chien-Ning
Published: February 2025

Cardiovascular–kidney–metabolic syndrome (CKMS) has become a significant global health challenge. Since CKMS often originates early in life, as outlined by the developmental origins of health and disease (DOHaD) concept, prevention is a more effective strategy than treatment. Various animal models, classified by environmental exposures or mechanisms, are used to explore the developmental origins of CKMS. However, no single model can fully replicate all aspects of CKMS or its clinical stages, limiting the advancement of preventive and therapeutic strategies. This review aims to assist researchers by comparing the strengths and limitations of common animal models used in CKMS programming studies and highlighting key considerations for selecting suitable models.
The Diagnostic Significance of SLC26A2 and Its Potential Role in Ulcerative Colitis
Authors: Qian Lijuan , Hu Shuo , Zhao Haizhou , Han Ye , Dai Chenguang , Zan Xinquan , Zhi Qiaoming , Xu Chunfang
Published: February 2025

Background/Objectives: The solute carrier family 26, member 2 (SLC26A2) gene, which belongs to the family of SLC26 transporters, can be detected in multiple tissues. However, the studies of SLC26A2 in colon-related diseases are still limited and incompletely understood, especially in ulcerative colitis (UC). Methods: In this study, we attempted to search and identify putative UC candidate genes within a large number of known genes by multiple bioinformatics analyses. The potential cellular characteristics and biological functions of SLC26A2 in the pathogenesis of UC were also elucidated. Results: Notably, SLC26A2 was representative and down-regulated in the intestinal mucosa of patients with active UC, compared to healthy controls. Decreased levels of SLC26A2 were proved to have a more value in diagnosis of UC patients, and closely correlated with some UC characteristics, including the Mayo score and Paediatric Ulcerative Colitis Activity Index (PUCAI). Mechanistically, subsequent results from published datasets and our validated clinical data all strongly implied that SLC26A2 was negatively correlated with the IL-17 signaling pathway, and positively associated with the tight junction, which led to abnormal immune cell infiltration and inflammatory injuries. After establishing the UC mice models in vivo by orally administration of DSS in portable water, SLC26A2 was significantly down-regulated at the mRNA or protein level, when compared to that in the control groups. Furthermore, the correlation analyses confirmed that SLC26A2 was positively associated with CLDN3, and negatively correlated with IL-17A expression in colon tissues. In addition, according to the SLC26A2 expression, UC patients were divided into different subgroups. The potential target drugs for UC treatment, such as progesterone, tetradioxin, and dexamethasone, were initially predicted and exerted anti-inflammatory effects via the common molecule-SLC26A2. Conclusions: SLC26A2 might be served as a protective candidate in the UC pathogenesis as well as a potential drug target for UC treatment.
Bioresorbable Materials for Wound Management
Authors: Lee Hye-Min , Ryu Hanjun
Published: February 2025

Chronic wounds pose a significant healthcare challenge due to their risk of severe complications, necessitating effective management strategies. Bioresorbable materials have emerged as an innovative solution, offering advantages such as eliminating the need for secondary surgical removal, reducing infection risks, and enabling time-delayed drug delivery. This review examines recent advancements in bioresorbable wound healing materials, focusing on a systematic review of bioresorbable materials, systems incorporating electrical stimulation, and drug delivery technologies to accelerate tissue repair. The discussion encompasses the fundamental principles of bioresorbable materials, including their resorption mechanisms and key properties, alongside preclinical applications that demonstrate their practical potential. Critical challenges impeding widespread adoption are addressed, and prospects for integrating these cutting-edge systems into clinical practice are outlined. Together, these insights underscore the promise of bioresorbable materials in revolutionizing chronic wound care.
Exploring the Validity of Adolescent Responses to a Measure of Psychological Flexibility and Inflexibility
Authors: Farley Caleb D. , Renshaw Tyler L.
Published: February 2025

Validating measures of psychological flexibility (PF) and psychological inflexibility (PI) has occurred in multiple adult samples, but little research has validated PF and PI measures with adolescents. This manuscript describes two studies exploring the validity of responses to the Multidimensional Psychological Flexibility Inventory (MPFI) with two samples of adolescents. The first study used exploratory factor analyses on responses to the MPFI with a sample of 16–17-year-olds (N = 249). The results yielded a reduced and simplified measurement model that consisted of two general factors: one for PF and the other for PI. These exploratory findings were further investigated with confirmatory factor analyses in the second study, with a larger sample of 14–17-year-olds (N = 503). The results from the second study generally confirmed the factor model from the first study. Findings from both studies showed that scores derived from the reduced MPFI measurement model evidenced convergent and divergent validity with a variety of mental health criterion measures. Moreover, findings from the second study showed that PF and PI scores had differential predictive power on different concurrent mental health outcomes. This discussion highlights the implications of measuring PF and PI in adolescents, considers limitations of the present studies, and recommends next steps for research.
The Impact of Nutrition Education, Strength Training, and Body Image Perception on Orthorexia Nervosa Risk: A Cross-Sectional Study in Women
Authors: Staśkiewicz-Bartecka Wiktoria , Tambor Laura , Kiciak Agata , Dobkowska-Szefer Daria , Kuczka Natalia , Białek-Dratwa Agnieszka , Bielaszka Agnieszka , Kowalski Oskar , Kardas Marek
Published: February 2025

Background: The increasing focus on healthy eating and achieving an ideal physique can lead to the development of disordered eating patterns, including orthorexia nervosa. The relationship between body image perception, self-esteem, and the risk of orthorexia nervosa is multifaceted, with negative body image and low self-esteem often acting as contributing factors. This study aimed to assess the risk of orthorexia nervosa and analyze body image perception among women engaged in strength training and dietetics students. Methods: The study was conducted using a Computer-Assisted Web Interview with 190 women aged 18 and older, divided into four groups: dietetics students engaged in strength training, dietetics students not engaged in strength training, non-dietetics women engaged in strength training, and non-dietetics women not engaged in strength training. The study utilized the Düsseldorf Orthorexia Scale to assess the risk of orthorexia nervosa and the Body-Esteem Scale to evaluate body image perception. Results: The highest orthorexia nervosa risk was observed in dietetics students who do not engage in a strength training group (60.9%), followed by dietetics students who engage in a strength training group (37.5%). In contrast, women who are not studying dietetics and do not engage in a strength training group exhibited the lowest orthorexia nervosa risk (13%), emphasizing the protective role of physical activity and reduced focus on nutritional rigidity. Regression analysis revealed that low appearance self-esteem (p = 0.011) and high social attribution (p = 0.043) significantly predicted higher orthorexia nervosa risk. Group affiliation also influenced orthorexia nervosa risk, with dietetics students showing higher Düsseldorf Orthorexia Scale scores. However, weight-related self-esteem (p = 0.082) did not significantly impact orthorexia nervosa scores. Conclusions: Dietetics education and physical activity independently and collectively affect orthorexia nervosa risk. Students in dietetics, particularly those not engaged in strength training, are at a higher risk due to the dual influence of academic pressures and heightened nutritional awareness. The findings underscore the need for interventions targeting body image perception, promoting flexible dietary approaches, and addressing external validation pressures to mitigate orthorexia nervosa risk in high-risk groups.
Measuring Circularity of Buildings: A Systematic Literature Review
Authors: dos Santos Gonçalves Joana , Claes Steven , Ritzen Michiel
Published: February 2025

The transition to a circular economy in the construction sector is crucial for reducing environmental impacts and resource depletion. However, a lack of harmonized methodologies and standardized indicators for measuring circularity remains a major challenge, hindering informed decision-making in the built environment. This study addresses this gap by systematically reviewing existing circularity assessment frameworks for buildings. A systematic literature review (SLR) was conducted following PRISMA guidelines, analyzing 948 records from major databases. The findings reveal gaps in current frameworks, particularly the fragmentation of indicators and an overemphasis on material flows, often neglecting adaptability, repairability, and maintainability. By mapping commonalities between indicator frameworks, data requirements, and aggregation methods, this study contributes to the harmonization of circularity assessment approaches, integrating multi-cycle considerations for buildings and construction products. The results in this research contribute to the development of comprehensive and practical assessment frameworks, facilitating the transition towards a more sustainable and circular built environment.
Full-Scale Testing and Stability Analysis of Prefabricated Steel Plate-Concrete Composite Walls in Underground Granaries
Authors: Zhang Hao , Wang Ruixin , Chen Lei , Chuai Jun
Published: February 2025

Underground granaries naturally preserve grain quality by maintaining low temperatures and reduced oxygen levels, eliminating the need for artificial cooling and pest control. However, cast-in-place reinforced concrete construction faces challenges such as waterproofing and complex on-site processes, necessitating prefabricated steel plate-concrete composite structures with robust joints for enhanced structural integrity and streamlined construction. The study utilizes a full-scale prefabricated steel plate-concrete underground silo, instrumented with strain gauges on circumferential steel bars and internal steel plates to monitor stress variations during six distinct backfilling loading cases. Concurrently, finite element models were developed using ABAQUS 6.14 software for numerical simulations, which were validated against experimental data. Stability analyses, including buckling load assessments and parameter sensitivity studies, were conducted to evaluate the effects of joint quantity and bending stiffness on the structural performance of the composite walls. The results revealed that circumferential joints play a critical role in stress distribution within the composite walls, underscoring the necessity of optimized joint design. The numerical model accurately replicated experimental results, with deviations below 9%, confirming its reliability. Furthermore, an equivalent joint design method was established, demonstrating that a joint bending stiffness ratio above 1.1 ensures that prefabricated composite walls achieve critical buckling loads comparable to cast-in-place walls. These findings provide a robust framework for enhancing the structural performance and reliability of prefabricated underground silos.
Transcription of Clock Genes in Medulloblastoma
Authors: Vriend Jerry , Glogowska Aleksandra
Published: February 2025

We investigated the transcription of circadian clock genes in publicly available datasets of gene expression in medulloblastoma (MB) tissues using the R2 Genomics Analysis and Visualization Platform. Differential expression of the core clock genes among the four consensus subgroups of MB (defined in 2012 as Group 3, Group 4, the SHH group, and the WNT group) included the core clock genes (CLOCK, NPAS2, PER1, PER2, CRY1, CRY2, BMAL1, BMAL2, NR1D1, and TIMELESS) and genes which encode proteins that regulate the transcription of clock genes (CIPC, FBXL21, and USP2). The over-expression of several clock genes, including CIPC, was found in individuals with the isochromosome 17q chromosomal aberration in MB Group 3 and Group 4. The most significant biological pathways associated with clock gene expression were ribosome subunits, phototransduction, GABAergic synapse, WNT signaling pathway, and the Fanconi anemia pathway. Survival analysis of clock genes was examined using the Kaplan–Meier method and the Cox proportional hazards regression model through the R2 Genomics Platform. Two clock genes most significantly related to survival were CRY1 and USP2. The data suggest that several clock proteins, including CRY1 and USP2, be investigated as potential therapeutic targets in MB.
Identifying High-Risk Patients with Advanced Midface Cancer: Personalized Surgical and Reconstructive Approach for Radical Resection
Authors: Bula Daniel , Opyrchał Jakub , Walczak Dominik , Krakowczyk Łukasz , Maciejewski Adam
Published: February 2025

Background: Individually personalized reconstructive microsurgery is more and more universally recognized and applied as a one-time, part of a few, or even the only highly effective treatment of patients with locally advanced midface cancer. Among the increasing number of publications focused on this field, most present different reconstructive techniques used for a single patient (case reports), and fewer papers evaluate a group including more than 100 patients. Methods: A dataset of 119 locally advanced midface cancers in stage T3 or T4 was used to analyze whether there is any correlation between clinical factors, resection defect parameters, and the free flaps chosen for reconstruction. Results: In general, the 5-year OS was 95% and 77% for the DFS, which inversely correlated with the increasing Cordeiro’s type of resective defects. Local recurrence occurred in 23% of cases. Taxonomic dendrograms allow the selection of four (A–D) different case clusters. Cluster B, which characterizes a tumor size of 8–18 cm2, Cordeiro type IIIA, and an uncertain radicalism of resection, has the worst prognosis with a very high (89%) risk of local recurrence. On the contrary, the most favorable was found in cluster C, characterized by Cordeiro type IIA, a tumor size of 8 cm2, and negative resective margins, because it has a very low (6%) risk of local recurrence. Conclusions: The results of the present analysis have led to design algorithms for midface resection and reconstruction. However, these should not be considered obligatory but rather as a useful general guideline.
Early Rectal Cancer: Advances in Diagnosis and Management Strategies
Authors: Mohammed Huda , Mohamed Hadeel , Mohamed Nusyba , Sharma Rajat , Sagar Jayesh
Published: February 2025

Colorectal cancer (CRC) is the second most prevalent cause of cancer-related death and the third most common cancer globally. Early-stage rectal cancer is defined by lesions confined to the bowel wall, without extension beyond the submucosa in T1 or the muscularis propria in T2, with no indication of lymph node involvement or distant metastasis. The gold standard for managing rectal cancer is total mesorectal excision (TME); however, it is linked to considerable morbidities and impaired quality of life. There is a growing interest in local resection and non-operative treatment of early RC for organ preservation. Local resection options include three types of transanal endoscopic surgery (TES): transanal endoscopic microsurgery (TEM), transanal endoscopic operations (TEO), and transanal minimally invasive surgery (TAMIS), while endoscopic resection includes endoscopic mucosal resection (EMR), underwater endoscopic mucosal resection (UEMR), and endoscopic submucosal dissection (ESD). Although the oncological outcome of local resection of early rectal cancer is debated in the current literature, some studies have shown comparable outcomes with radical surgery in selected patients. The use of adjuvant and neoadjuvant chemoradiotherapy in early rectal cancer management is also controversial in the literature, but a number of studies have reported promising outcomes. This review focuses on the available literature regarding diagnosis, staging, and management strategies of early rectal cancer and provides possible recommendations.
Ultrasonic Aspiration-Acquired Glioblastoma Tissue Preserves Lymphocyte Phenotype and Viability, Supporting Its Use for Immunological Studies
Authors: Stavrakaki Eftychia , Belcaid Zineb , Balvers Rutger K. , Vogelezang Lisette B. , van den Bossche Wouter B. L. , Alderliesten Demi , Lila Karishma , van den Bosch Thierry P. P. , van Dongen Jacques J. M. , Debets Reno , Teodosio Cristina , Dirven Clemens M. F. , Lamfers Martine L. M.
Published: February 2025

Background and Objective: Access to high-quality patient-derived brain tumor tissues is instrumental for translational neuro-oncology research. Glioblastoma tumor material resected by ultrasonic aspiration (UA) during surgery offers an abundant source of material; however, it is generally not used for research experiments. We hypothesize that UA-derived tumor tissue represents a source of tissue that accurately reflects the immune infiltrates of glioblastomas. Methods: In this study, we have utilized UA-derived tissue and performed a head-to-head comparison with paired resection tissue from the vital tumor core of the same patient. A combination of 16 fluorochrome-conjugated antibodies was designed to identify tumor-infiltrating T, B, and NK lymphocytes and characterize the TILs by spectral flow cytometry. Furthermore, a 5-plex panel was designed to spatially characterize the T cells, macrophages, and tumor cells on the paired UA and resection tissues. Results: UA-obtained cells exhibited a comparable yield and viability, as well as an abundance of tumor-infiltrating T, B, and NK lymphocytes compared to resection sample-derived cells. Importantly, we observed that there is a high concordance with respect to expression intensities of immune checkpoints by T cells in both types of tissue samples. Conclusions: These findings underscore the feasibility and reliability of utilizing the immune infiltrates from ultrasonic aspiration-acquired glioblastoma tissue.
Atypical Pelvic Tumors in Children
Authors: Sobieraj Paulina , Bekiesińska-Figatowska Monika
Published: February 2025

Due to the complex anatomy of the pelvis, various tumors may arise in this region. Some of these tumors are well known and have distinctive features that allow them to be identified by magnetic resonance imaging (MRI). These include sacrococcygeal teratoma (SCT), the most prevalent congenital tumor in children, often diagnosed prenatally and most frequently occurring in this anatomical location, and ovarian teratoma, which in its mature form is the most common ovarian neoplasm in children and adolescents. Additionally, rhabdomyosarcoma (RMS), commonly found in the bladder in both genders and in the prostate in males, and Ewing sarcoma (ES), affecting the flat bones of the pelvis, are relatively common tumors. In this study, selected atypical pelvic tumors in children are presented. Most of them are tumors of the reproductive system, such as cervical cancer, small cell neuroendocrine carcinoma of the ovary, ES/primitive neuroectodermal tumor (PNET) of the ovary, diffuse large B-cell lymphoma (DLBCL) of the ovaries and ovarian Sertoli–Leydig cell tumor (SLCT) with RMS due to DICER1 syndrome. Additionally, tumors originating from the nervous system, including neuroblastoma (NBL) and plexiform neurofibroma (pNF), associated and not associated with neurofibromatosis type 1 (NF1), are discussed. Furthermore, Rosai–Dorfman disease involving the pelvic and inguinal lymph nodes is presented. By reviewing the literature and presenting our cases, we tried to find radiological features of individual tumors that would bring the radiologist closer to the correct diagnosis, ensuring the implementation of appropriate treatment. However, the MR images cannot be considered in isolation. Additional patient data, such as the clinical picture, comorbidities/syndromes, and laboratory test results, are necessary.
Integrative Analysis of Metabolome and Proteome in the Cerebrospinal Fluid of Patients with Multiple System Atrophy
Authors: George Nimisha Pradeep , Kwon Minjun , Jang Yong Eun , Kim Seok Gi , Hwang Ji Su , Lee Sang Seop , Lee Gwang
Published: February 2025

Multiple system atrophy (MSA) is a progressive neurodegenerative synucleinopathy. Differentiating MSA from other synucleinopathies, especially in the early stages, is challenging because of its overlapping symptoms with other forms of Parkinsonism. Thus, there is a pressing need to clarify the underlying biological mechanisms and identify specific biomarkers for MSA. The metabolic profile of cerebrospinal fluid (CSF) is known to be altered in MSA. To further investigate the biological mechanisms behind the metabolic changes, we created a network of altered CSF metabolites in patients with MSA and analysed these changes using bioinformatic software. Acknowledging the limitations of metabolomics, we incorporated proteomic data to improve the overall comprehensiveness of the study. Our in silico predictions showed elevated ROS, cytoplasmic inclusions, white matter demyelination, ataxia, and neurodegeneration, with ATP concentration, neurotransmitter release, and oligodendrocyte count predicted to be suppressed in MSA CSF samples. Machine learning and dimension reduction are important multi-omics approaches as they handle large amounts of data, identify patterns, and make predictions while reducing variance without information loss and generating easily visualised plots that help identify clusters, patterns, or outliers. Thus, integrated multiomics and machine learning approaches are essential for elucidating neurodegenerative mechanisms and identifying potential diagnostic biomarkers of MSA.
Lanthanide-Doped Upconversion Luminescence: A New Frontier in Pathogenic Bacteria and Metabolite Detection from Design to Point-of-Care Application
Authors: Li Huanhuan , Wu Yu , Shoaib Muhammad , Sheng Wei , Bei Qiyi , Murugesan Arul
Published: February 2025

Pathogens and their metabolites in food present significant risks to both human health and economic development. Rising living standards and increasing awareness of food safety have driven the demand for sensitive and rapid detection methods. Lanthanide-doped upconversion nanoparticles (UCNPs), with their exceptional optical properties, have emerged as a promising platform for developing biosensors to detect pathogenic bacteria and their metabolites. The integration of UCNPs with point-of-care testing (POCT) has garnered considerable attention for its portability and immediacy, highlighting a promising future for biosensing, particularly in applications requiring quick and accurate diagnostics. This review explores the recognition elements and design principles commonly used in UCNP-based biosensors and examines various applications, including lateral flow assays, microfluidic systems, photoelectrochemical devices, and smartphone-integrated platforms. Despite significant advancements, challenges remain in the applicability and commercialization of UCNP-based biosensing technology. Future research should focus on enhancing sensitivity and specificity, developing scalable and cost-effective production methods, and integrating with advanced digital technologies to enable broader adoption. Addressing these challenges, establishing regulatory frameworks, and considering sustainability will be crucial to fully realizing the potential of UCNP-based biosensors.
Electrochemical Sensors and Biosensors for the Detection of Pharmaceutical Contaminants in Natural Waters—A Comprehensive Review
Authors: Bounegru Alexandra Virginia , Dinu Iacob Ancuța , Iticescu Cătălina , Georgescu Puiu Lucian
Published: February 2025

This review highlights recent advances in the development of electrochemical sensors and biosensors for detecting pharmaceutical contaminants in water samples, including surface water, wastewater, and bottled water. The electrode modification materials—such as nanomaterials, conductive polymers, and eco-friendly nanocomposites—have shown notable improvements in sensor sensitivity and selectivity, enabling the detection of various pharmaceutical compounds, including diclofenac, ibuprofen, antibiotics, and hormones. Future research directions suggest the use of eco-friendly nanocomposites, the development of multiplexed platforms for simultaneous contaminant detection, and the integration of sensors into portable devices for in situ monitoring. Additionally, integrating biosensors with specific biomolecules and IoT technology can expand their applicability, enabling continuous and efficient water quality monitoring, thus supporting public health and environmental protection.
Constructing Micro-/Nano-Aramid Pulp (MAP)–Epoxy Coatings on Laser-Engraved Titanium Alloy Surfaces for Stronger Adhesive Bonding with Carbon Fiber-Reinforced Polymer Panel
Authors: Zhu Haibo , Cheng Fei , Zuo Shihao , Zhang Jinheng , Huang Wenyi , Fan Tangrui , Hu Xiaozhi
Published: February 2025

A shape-controllable laser-engraving treatment (LET) and aramid pulp (AP)-reinforced resin pre-coating (RPC) were used on a titanium (Ti) alloy surface to construct micro-/nano-aramid pulp and epoxy (MAPE) coatings for greater bonding strength with carbon fiber-reinforced polymers (CFRPs). The array pits of regular hexagon on the Ti alloy surface were engraved and vertical spaces between the array pits were created to place the AP-reinforced epoxy for stronger mechanical interlocking. The specimen treated with laser engraving (side length of 0.3 mm) and AP-reinforced RPC yielded the greatest bonding strength of 27.1 MPa, 67.4% higher than the base strength. The failure modes of the Ti-CFRPs composites changed from debonding failure at the Ti/epoxy surface to fiber-damaged failure of the laminated CFRPs panels. The shape-controllable LET and simple AP-reinforced RPC were confirmed as the most feasible and effective combined methods for use on titanium alloy surfaces for manufacturing stronger Ti-CFRPs composites, which exhibited the potential for application in other metal–matrix-bonding composite systems.
Thermal Friction Contact Analysis of Graded Piezoelectric Coatings Under Conductive Punch Loading
Authors: Zhou Xinyu , Liu Jing , Mao Jiajia
Published: February 2025

In this paper, we investigate the thermal friction sliding contact of a functionally graded piezoelectric material (FGPM)-coated half-plane subjected to a rigid conductive cylindrical punch. This study considers the effect of the thermal convection term in heat conduction. The thermo-electro-elastic material parameters of the coating vary exponentially along its thickness direction. Utilizing thermoelastic theory and Fourier integral transforms, the problem is formulated into Cauchy singular integral equations of the first and second kinds with surface stress, contact width, and electric displacement as the unknown variables. The numerical solutions for the contact stress, electric displacement, and temperature field of the graded coating surface are obtained using the least-squares method and iterative techniques. It can be observed that the thermo-electro-elastic contact behavior of the coating surface undergoes significant changes as the graded index varies from −0.5 to 0.5, the friction coefficient ranges from 0.1 to 0.5, and the sliding velocity changes from 0.01 m/s to 0.05 m/s. The results indicate that adjusting the graded index of the coating, the sliding speed of the punch, and the friction coefficient can improve the thermo-electro-elastic contact damage of the material’s surface.
Outcomes of Robot-Assisted Transbronchial Biopsies of Pulmonary Nodules: A Review
Authors: Ebeling Peter A. , Daouk Salim , Keddissi Jean I. , Youness Houssein A.
Published: February 2025

Background/Objectives: Robot-assisted bronchoscopy (RAB) is a novel platform for sampling peripheral pulmonary nodules (PPNs). To further clarify the role robot-assisted platforms have in diagnosing PPNs, we performed a review of the recent literature. Methods: A systematic review was performed in Medline from 2019 to 2024 using the search terms “robotic bronchoscopy”, “diagnostic yield”, “sensitivity”, and “positive predictive value”, alone and in combination. Studies that focused on earlier electromagnetic bronchoscopies were excluded. The patient demographic information, nodule characteristics, intra-procedure imaging modality, biopsy methods, diagnostic yield, sensitivity for malignancy, and adverse outcomes were analyzed. A total of 22 studies were available for the analyses. Results: The diagnostic yield was variable and ranged from 69 to 93%, with a median of 86%. The sensitivity ranged from 69% to 91.7%, with a median of 85%. The effect of the nodule size on the diagnostic yield was variable across the literature. Obtaining an eccentric or concentric view on a radial endobronchial ultrasound (rEBUS) was associated with a higher diagnostic yield than obtaining no view. A nodule appearance on CT imaging and the location were not definitively associated with a higher diagnostic yield. Fine needle aspiration usage ranged from 93.5 to 100%, with a median of 96.95%, while the use of biopsy forceps ranged from 2.7 to 96%, with a median of 69.9%. The most common complication was a pneumothorax, which occurred in 1–5.7% of cases, with a median of 1.6%. Conclusions: Robot-assisted transbronchial biopsies produce diagnostic yields that approach those of transthoracic needle aspirations. The nodule location and appearance may not affect the diagnostic yield. Obtaining a concentric or eccentric view on rEBUS is likely associated with an increased diagnostic yield. Additional prospective studies would better inform practitioners as this technology becomes more widespread.
Photobiomodulation LED Devices for Home Use: Design, Function and Potential: A Pilot Study
Authors: Cronshaw Mark , Parker Steven , Hamadah Omar , Arnabat-Dominguez Josep , Grootveld Martin
Published: February 2025

Background/Objectives: Many commercial light-emitting diode (LED) devices are available for consumer home usage. The performance characteristics in respect to the dosimetry of many of the devices, currently on direct sale to the public, have not been subject to formal appraisal. In order to ‘bridge the gap’ between the evidence-based photobiomodulation therapy (PBMT) community and other interested parties, an evaluation is made of a selection of torch type hand-held LED PBMT products currently available for home use. Methods: Five randomly chosen intra-oral and hand-held LED PBMT devices were selected. The optical delivery parameters of the devices were measured, including the beam divergence angle, surface area exposure as well as the output power at the level of the LEDs. The surface and sub-surface temperature changes in porcine tissue samples were assessed under standardised conditions. The manufacturer’s patient instructions were correlated to the measured optical parameters. Calculations were made of irradiance and surface radiant exposure. Consumer satisfaction ratings and feedback data were collated, and a relevant statistical analysis conducted. Results: The results were heterogeneous with a wide range of applied wavelengths, output power and irradiance. Power output stability was variable, and, together with a wide beam divergence angle of 74°, the manufacturer’s directions for dosimetry were found to be inconsistent with an accurate dose delivery. Conclusions: The manufacturer’s proposed dosimetry fails to consider the relevance of the beam divergence angle and optical attenuation in view of the scatter and absorption. Appropriate instructions on how best to gain and optimise an acceptable clinical outcome were inconsistent with an evidence-based approach. Subject to validation by well-planned clinical trials, the concept of home PBMT may open interesting new therapeutic approaches.
Topical Oxygenation Improves Microvascularity in a Human Ex Vivo Palatal Tissue Model: A Histological Analysis
Authors: Pilloni Andrea , Di Gioia Cira Rosaria Tiziana , Carletti Raffaella , D’Elia Gerarda , Di Mario Michaela , Molendini Angela , Romeo Umberto , Marini Lorenzo
Published: February 2025

Background: Several therapies involving the use of oxygen have been developed; however, the literature to date has poorly addressed the effects of Topical Oxygen Therapy (TOT) on oral tissues. The aim of the present pilot study was to histologically evaluate the response to TOT in epithelial–connective samples harvested from the palate. Methods: In eight patients selected for a free gingival graft (FGG) procedure, the lateral portions of the graft were randomly assigned to receive TOT (test) or no treatment (control). Both the portions were stained with hematoxylin–eosin for the evaluation of histomorphological changes and with Picrosirius for the study of collagen. Immunohistochemical staining was performed with antibodies for the characterization of the inflammatory infiltrate and neoangiogenesis. Results: The analysis failed to show morphological variations in both groups, demonstrating that there was no tissue damage due to TOT. The prevalence of type I collagen in both samples supported this finding. Immune cells were present in low levels and mainly consisted of CD3+ T lymphocytes. The histomorphometric analysis showed an increased average vessel area (7607.95 μm2 ± 3983.24 vs. 4038.42 μm2 ± 1823.52), an increased number and caliber of vessels (49.82 ± 20.55 vs. 32.35 ± 16.64), and a higher microvessel density (7.89 ± 3.25 vessels/0.26 mm2 vs. 5.13 ± 2.63 vessels/0.26 mm2) in the test group. Conclusions: Although further investigations are needed, TOT could improve and speed up oral wound healing through the local condition of hyperoxia.
Contribution of Androgen Receptor CAG Repeat Polymorphism to Human Reproduction
Authors: Ciarloni Alessandro , delli Muti Nicola , Ambo Nicola , Perrone Michele , Rossi Silvia , Sacco Sara , Salvio Gianmaria , Balercia Giancarlo
Published: February 2025

Background: Exon 1 of the gene encoding for the androgen receptor (AR) contains a polymorphic sequence of variably repeated CAG triplets ranging from 11 to 36. The number of triplets appears to inversely correlate with receptor transcriptional activity, conditioning the peripheral effects of testosterone. Methods: We conducted a narrative review to explore the current evidence regarding the relationship between the number of CAG repeats and the human reproductive system. Results: We found several articles that investigate the relationship between CAG polymorphism and the male reproductive system, suggesting a possible modulatory effect on spermatogenesis, sexual function, prostate cancer, and testicular cancer. Similarly, in women, evidence has emerged to support a possible relationship between CAG repeat number and breast cancer, polycystic ovary syndrome (PCOS), and recurrent spontaneous abortions (RSAs). Unfortunately, the data in the current literature are largely discordant, largely due to an important influence of ethnicity on the variability of the CAG polymorphism, and partly due to the quality of the available studies. Conclusions: In the current state of the art, the study of CAG polymorphism does not have a sufficient literature base to allow its use in common clinical practice. However, it represents an interesting research target and, in the future, as new evidence emerges, it could help to elucidate some pathogenetic aspects of human reproductive disorders.
Alkali Halide Aqueous Solutions Under Pressure: A Non-Equilibrium Molecular Dynamics Investigation of Thermal Transport and Thermodiffusion
Authors: Zhao Guansen , Bresme Fernando
Published: February 2025

Thermal gradients induce thermodiffusion in aqueous solutions, a non-equilibrium effect arising from the coupling of thermal and mass fluxes. While thermal transport processes have garnered significant attention under standard conditions, thermal transport at high pressures and temperatures, typical of the Earth’s crust, has escaped scrutiny. Non-equilibrium thermodynamics theory and non-equilibrium molecular dynamics simulations provide an excellent means to quantify thermal transport under extreme conditions and establish a connection between the behaviour of the solutions and their microscopic structure. Here, we investigate the thermal conductivity and thermal diffusion of NaCl and LiCl solutions in the GPa pressure regime, targeting temperatures between 300 K and 1000 K at 1 molal concentration. We employ non-equilibrium molecular dynamics simulations along with the Madrid-2019 and TIP4P/2005 force fields. The thermal conductivity of the solutions increases significantly with pressure, and following the behaviour observed at standard pressure, the thermal conductivity is lower than that of pure water. The reduction in thermal conductivity is significant in the GPa pressure regime, ∼3% for 1 molal NaCl and LiCl solutions. We demonstrate that under GPa pressure conditions, the solutions feature thermophobic behaviour, with ions migrating towards colder regions. The pronounced impact of pressure is more evident in LiCl solutions, which display a thermophilic to thermophobic “transition” at pressures above 0.25 GPa. We discuss a correlation between the solution’s thermophobicity and the disruption of the water hydrogen bond structure at high pressure, where the water structure resembles that observed in simple liquids.