bluuebunny/crossref_metadata_2025 huggingface dataset:
THE ENFORCEMENT OF VIRTUE
Authors: Buckley F. H.
Published: May 2019

Corruption of public officials is the silent killer of the U.S. economy, and we should reflect carefully on how it might be reined in. That’s the thought behind campaign finance laws. But broad grants of discretion to authorities, which might work in New Zealand, are more likely to be abused in low-trust America, and campaign finance laws are one example of this. First Amendment free speech rights, as interpreted by the Supreme Court, represent its reflection on the American character and the possibility of abuse when Congress tries to restrict political speech. So conservatives are apt to think, and not entirely without reason.
A Site-Level Market Model of the Antiquities Trade
Authors: Greenland Fiona , Marrone James V. , Topçuoğlu Oya , Vorderstrasse Tasha
Published: May 2019

Archaeological looting correlates with a number of problems, including the destruction of stratigraphic data and the damage and loss of artifacts. Looting is also understood to generate revenue, but systematic analysis of this issue is challenged by its opacity: how can we study the economic effects of archaeological looting when the practice is rarely directly observable? To address this problem, we estimate the market value of archaeological sites where artifacts have been previously excavated and documented, using a machine-learning approach. The first step uses 41,587 sales of objects from 33 firms to train an algorithm to predict the distribution channel, lot packaging, and estimated sale price of objects based on their observable characteristics. The second step uses the trained algorithm to estimate the value of sites in which a large number of artifacts have been legally excavated and documented. We make an out-of-sample prediction on two Syrian sites, Tell Bi’a and Dura Europos.
“Save Archaeology from the Technicians”: Wheeler, World Heritage and Expert Failure at Mohenjodaro
Authors: Meskell Lynn
Published: May 2019

Between the 1960s and 1980s, the United Nations Educational, Scientific and Cultural Organization (UNESCO) launched a number of international salvage campaigns at the behest of its member states, the most notable being the Nubian Monuments Campaign. Saving ancient monuments and discovering archaeological sites in Egypt and Sudan was viewed as a landmark in twentieth-century heritage conservation and international co-operation; it was never to be repeated again. Requests for assistance continued, yet the campaigns were configured as conservation missions rather than integrated field projects. In retrospect, this action delimited UNESCO’s goals to bridge education, science, and culture and, ultimately, impacted the potential of cross-cultural dialogue and partnerships afforded by archaeological field research. This article focuses on UNESCO’s International Campaign for the Safeguarding of Moenjodaro (1974–97) and traces the role of experts, both archaeological and hydrological, within UNESCO’s mission. It uncovers the organization’s handling of the monumental challenges and long-term effects of a multimillion-dollar salvage effort. In doing so, the article reveals the early role of prominent archaeologists Mortimer Wheeler and John Otis Brew, the ideological shift at UNESCO from archaeological research to monumentality and preservation, and the tensions that emerged as world heritage was reimagined.
The Bangwa Queen: Artifact or Heritage?
Authors: Campfens Evelien
Published: May 2019

The return of cultural objects lost as a result of colonial rule is a controversial issue. A common response is: “it was legal at the time” and, therefore, not a legal issue. But is that so? This article argues that it is not a lack of legal norms that explains this belated discussion but, rather, the asymmetrical application of norms. Moreover, a human rights law approach, focusing on the heritage aspect of cultural objects for people today—instead of a sole focus on property title—offers useful tools to structure this field. To illustrate these points, a case concerning an African ancestral sculpture today known as the “Bangwa Queen” will be assessed on its merits under international law. The Bangwa Queen is of spiritual importance to the Bangwa, a people indigenous to the western part of Cameroon. She was taken as part of a collection of so-calledlefemfigures by German colonizers in 1899 and is currently part of a French museum collection.
Transmission dynamics of foot and mouth disease in selected outbreak areas of northwest Ethiopia
Authors: Tadesse B. , Molla W. , Mengsitu A. , Jemberu W. T.
Published: May 2019

Foot and mouth disease (FMD) is a highly contagious and economically important disease of cloven-hoofed animals, which is endemic in Ethiopia. An outbreak follow-up study was undertaken to quantify the transmission parameters of FMD in the crop–livestock mixed (CLM) system and commercial dairy farms in selected areas of northwest Ethiopia. The transmission parameters were quantified using a generalised linear model (GLM) based on a susceptible–infectious–recovered (SIR) epidemic model. The per day average transmission rate between animals was 0.26 (95% CI 0.22–0.32) and 0.33 (95% CI 0.21–0.57) in the CLM system and in the commercial dairy farms, respectively. The average basic reproduction ratio of FMD was 1.68 (95% CI 1.42–2.07) in the CLM system and 1.98 (95% CI 1.26–3.42) in the commercial dairy farms. The medium per day transmission rate and moderate basic reproduction ratio observed in this study indicated that a vaccination coverage needed to stop transmission of the disease in these populations might not be very high.
Genetic variants in RIG-I-like receptor influences HCV clearance in Chinese Han population
Authors: Wu Xinyu , Zang Feng , Liu Mei , Zhuo Lingyun , Wu Jingjing , Xia Xueshan , Feng Yue , Yu Rongbin , Huang Peng , Yang Sheng
Published: May 2019

Human innate immune plays an essential role in the spontaneous clearance of acute infection and therapy of HCV. We investigated whether the SNPs in retinoic acid-inducible gene I-like receptor family were associated with HCV spontaneous clearance and response to treatment. To evaluate the clinical value of DDX58 rs3824456, rs10813831 and rs10738889 genotypes on HCV spontaneous clearance and treatment response in Chinese Han population, we genotyped 1001 HCV persistent infectors, 599 participants with HCV natural clearance and 354 patients with PEGylated interferon-α and ribavirin (PEG IFN-α/RBV) treatment. People carrying rs10813831-G allele genotype were more liable to achieve spontaneous clearance than the carriage of the T allele (dominant model: adjusted OR 1.35, 95% CI 1.08–1.71, P = 0.008). In rs10738889, the rate of persistent infection was significantly lower in patients with the TC genotype compared to those with TT genotype (dominant model: adjusted OR 1.36, 95% CI 1.06–1.74, P = 0.015). Multivariate stepwise analysis indicated that rs10738889, age, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were independent predictors for HCV spontaneous clearance. However, there were no significant differences in the three selection SNPs between the non-SVR group and the SVR group. These results suggest the DDX58 rs10813831 and rs10738889 are associated with spontaneous clearance of HCV, which may be identified as a predictive marker in the Chinese Han population of HCV.
The epidemiology of paediatricMycoplasma pneumoniaepneumonia in North China: 2006 to 2016
Authors: Gao Li-Wei , Yin Ju , Hu Ying-hui , Liu Xiu-yun , Feng Xue-li , He Jian-Xin , Liu Jun , Guo Yan , Xu Bao-Ping , Shen Kun-Ling
Published: May 2019

PaediatricMycoplasma pneumoniaepneumonia (MPP) is a major cause of community-acquired pneumonia in China. Data on epidemiology of paediatric MPP from China are little known. This study retrospectively collected data from June 2006 to June 2016 in Beijing Children's Hospital, Capital Medical University of North China and aims to explore the epidemiological features of paediatric MPP and severe MPP (SMPP) in North China during the past 10 years. A total of 27 498 paediatric patients with pneumonia were enrolled. Among them, 37.5% of paediatric patients had MPP. In this area, an epidemic took place every 2–3 years at the peak, and the positive rate of MPP increased during these peak years over time. The peak age of MPP was between the ages of 6 and 10 years, accounting for 75.2%, significantly more compared with other age groups (χ2= 1384.1,P< 0.0001). The epidemics peaked in September, October and November (χ2= 904.9,P< 0.0001). Additionally, 13.0% of MPP paediatric patients were SMPP, but over time, the rate of SMPP increased, reaching 42.6% in 2016. The mean age of paediatric patients with SMPP (6.7 ± 3.0 years old) was younger than that of patients with non-SMPP (7.4 ± 3.2 years old) (t= 3.60,P= 0.0001). The prevalence of MPP and SMPP is common in China, especially in children from 6 to 10 years old. Paediatric patients with SMPP tend to be younger than those with non-SMPP. MPP outbreaks occur every 2–3 years in North China. September, October and November are the peak months, unlike in South China. Understanding the epidemiological characteristics of paediatric MPP can contribute to timely treatment and diagnosis, and may improve the prognosis of children with SMPP.
Lacustrine systems of Clearwater Mesa (James Ross Island, north-eastern Antarctic Peninsula): geomorphological setting and limnological characterization
Authors: Roman Matěj , Nedbalová Linda , Kohler Tyler J. , Lirio Juan M. , Coria Silvia H. , Kopáček Jiří , Vignoni Paula A. , Kopalová Kateřina , Lecomte Karina L. , Elster Josef , Nývlt Daniel
Published: May 2019

Lentic freshwater habitats are important centres of biodiversity within the infrequent ice-free oases across Antarctica. Given imminent climate changes, it is crucial to catalogue these habitats in order to provide baseline data for future monitoring and biological surveys. The lacustrine systems of Clearwater Mesa, a previously unexplored part of James Ross Island, north-eastern Antarctic Peninsula, are described here. We conducted basic geomorphological and limnological surveys over three Antarctic summers (2009–16) to characterize landscape evolution, infer the origin of lake basins and assess the variability in their water chemistry. Stable shallow lakes, formed in depressions between lava tumuli following the last deglaciation, were found to dominate the volcanic mesa, although several peripheral lakes in ice-proximal settings appear to have formed recently as a result of post-Neoglacial ice recession. We found large heterogeneity in conductivity (~10–7000 μS cm−1), despite the lithologically uniform substrate. This variability was shown to be related to lake type, basin type (openvsclosed), meltwater source and proximity to the coast. Inter-annual differences were attributed to changes in sea spray influx and snow accumulation driven by variable weather conditions. Overall, the ion composition of lakes suggested that sea spray was the dominant source of ions, followed by the weathering of bedrock.
Seed dormancy and longevity variability of Hirschfeldia incana L. during storage
Authors: Mira Sara , Veiga-Barbosa Luciana , Pérez-García Félix
Published: May 2019

We studied the variability of germination, dormancy and viability loss of Hirschfeldia incana seeds in relation to seed size. Seeds were stored at 35°C under humid [75% relative humidity (RH)] or dry (33% RH) conditions. Seed germination and electrolyte leakage were evaluated periodically. Small seeds had lower longevity at humid or dry storage conditions (5 or 407 days, respectively) than large or intermediate seeds (7–9 or 536–727 days, respectively). Moreover, H. incana shows variability in seed dormancy related to seed size within a population, with small seeds having lower dormancy (13%) than intermediate (50%) or large seeds (72%). Dormancy was partially released after a short storage at 35°C and humid conditions. Under dry storage conditions, endogenous dormancy cycles were observed for over a year, and longer times of storage had a dormancy-breaking effect through dry after-ripening. Results suggest a dual strategy producing non-dormant seeds with low longevity that will germinate immediately after dispersal, and seeds with greater longevity that will delay germination. Membrane permeability increased linearly with ageing at both humid and dry storage (R2 = 0.60). Small seeds showed greater conductivity than intermediate or large seeds (0.7, 0.4 or 0.3 mS g–1 dry weight, respectively, at the 80% germination). The conductivity test could be used to evaluate the quality of H. incana seeds and would allow us to identify dormant (non-germinating) seed lots as viable. However, the influence of storage conditions and variability within a seed population on seed longevity should be taken into account when evaluating seed quality.
James Fitzjames Stephen and the Landscape of Victorian Political Thought
Authors: Conti Greg
Published: May 2019

In 1858 an aged and weakened James Stephen, the once-formidable “Over-Secretary of the Colonies” whose influence on the course of British imperial administration included such momentous tasks as drafting the bill to end slavery in the colonies and contributing to much of the administrative–constitutional groundwork for colonial self-government, wrote his son James Fitzjames words of encouragement on his rising writing career: “Time was when I enjoyed a repute as a writer of Edinburgh Reviews and from the bottom of my heart I hope as I sincerely believe that you will eclipse me even more than the elder Mill has been eclipsed by the younger.” For all the power he had exercised in the Colonial Office, and for all the worldly success that Fitzjames might enjoy in the legal profession he had been practicing for a half-decade at that point, there was something unique about literary fame that James wished his son to have.
Yellow mealworm (Tenebrio molitorL.) larvae inclusion in diets for free-range chickens: effects on meat quality and fatty acid profile
Authors: Dabbou S. , Gasco L. , Lussiana C. , Brugiapaglia A. , Biasato I. , Renna M. , Cavallarin L. , Gai F. , Schiavone A.
Published: May 2019

This study evaluated the effects of a diet containing yellow mealworm (Tenebrio molitorL.; TM) larva meal on quality parameters (pH24, color and drip losses), proximate composition and fatty acid (FA) profile of meat from free-range chickens. A total of 140 medium-growing hybrid female chickens were free-range reared and randomly allotted to two dietary treatments: a control group and a TM group, in which TM meal was included at 75 g kg−1as fed in substitution of corn gluten meal. Each group consisted of five pens as replicates, with 14 chicks per pen. At 97 days of age, ten birds (two birds/pen) from each feeding group were slaughtered at a commercial abattoir. Quality parameters and proximate composition of breast and thigh meat were not affected by treatment. The effects of dietary TM larva meal on the FA profile of thigh meat were negligible. Breast meat from TM-fed chickens showed higher oleic and α-linolenic acid percentages as well as lower atherogenicity and thrombogenicity indexes. In conclusion, this study demonstrated that TM inclusion in diets for free-range chickens did not prejudice meat quality traits. The obtained results confirm that TM can be considered a promising insect protein source for the poultry feed industry.
Institutional transformation and development from an economic transition perspective: the case of Argentina
Authors: Perényi Áron , Esposto Alexis , Bamforth Jill
Published: May 2019

Economic reforms and political changes often take place simultaneously. The outcomes of reforms in transitional countries depend on the sequence of these changes. Kornai developed his transition framework based on Central and Eastern Europe, and extended it to Asian nations. This article builds on Kornai's model and the institutional dialogue of economic development to construct and apply a model of transformation for Latin America. The aim is to better explain the relationship between institutional transformation and developmental outcomes over time. Our analysis utilises historical information and the views of current political leaders in Argentina. The analysis performed reflects on institutional performance, and evaluates the applicability of the proposed Latin American transition framework in practice. Based on the empirical evidence presented, we argue that economic transition is a path-dependent phenomenon, and that our model explaining the interaction of economic and political changes provides an explanation for the performance of institutional transformation in Argentina.
Proof‐of‐Concept Modeling of a New Groundwater Sampling Approach
Authors: Hou Deyi , Luo Jian
Published: May 2019

This note provides a proof‐of‐concept modeling study to examine the theoretical feasibility of a new groundwater sampling approach, the high‐stress low‐flow approach, for improving the representativeness of groundwater samples and shortening the sampling duration. The approach consists of two‐phase pumping: an initial high pumping rate followed by low‐flow purging and sampling. We conceptualize the sampling process by pumping in a fully penetrating, large‐diameter well in a confined aquifer and mixing within the well screened zone, which are characterized by the aquifer‐responding time scale for aquifer water dominating the inflow ratio and the well‐mixing time scale, respectively. We derive an analytical solution to evaluate the sample concentration and representativeness. Results show that higher pumping rates in phase 1 create larger drawdowns and overstressed flow fields when switching to lower pumping rates in phase 2, thereby slowing down the drawdown within the well and preventing downward movement of the well casing water. As a result, the ratio of aquifer water into the well screened zone and in water pumped can rise sharply at the pumping‐switch moment, and the aquifer‐responding time scale and groundwater sampling duration can be significantly shortened. The proposed high‐stress low‐flow approach is particularly effective in systems limited by long aquifer‐responding time scales, which are typically encountered in low‐permeability aquifers. Based on the analytical solution, we also provide preliminary guidelines and graphic tools to select the pumping rate ratio and the switching time. Our proof‐of‐concept modeling study demonstrates great potential of the new groundwater sampling approach in field applications.
Where Did All the Free-Flowing Rivers Go?
Authors: Duncombe Jenessa
Published: May 2019

A map of the world’s free-flowing rivers shows a shrinking number can still meander as they please. New plans for hydropower will further constrain flow.
Short‐ and Long‐Term Variations in the Reykjanes Geothermal Reservoir From Seismic Noise Interferometry
Authors: Sánchez‐Pastor Pilar , Obermann Anne , Schimmel Martin , Weemstra Cornelis , Verdel Arie , Jousset Philippe
Published: May 2019

The Reykjanes Geothermal System (RGS) is a high‐temperature geothermal system located on the Reykjanes peninsula, a transtensional plate‐boundary zone located on the southwestern tip of Iceland. The area is characterized by high seismicity, recent volcanism, and high‐temperature geothermal fields. We use seismic noise records from April 2014 to August 2015 to study stress changes and potential deformation of the subsurface caused by injection and production operations at RGS through seismic interferometry. We retrieve continuous time series of waveform similarity values and seismic velocity changes during this period. The S‐transform of the similarity values allows us to clearly identify three variations in the mechanical properties of the Reykjanes peninsula related to rapid changes of RGS production. In addition, we observe a slow seismic velocity decrease of 0.36%/year in the reservoir due to the water deficit and seasonal variations associated with the energy production demand.
Network Effects and Handling of the Geocenter Motion in Multi‐GNSS Processing
Authors: Zajdel R. , Sośnica K. , Dach R. , Bury G. , Prange L. , Jäggi A.
Published: May 2019

Both, the network configuration and the way of terrestrial reference frame (TRF) realization, affect the global geodetic products delivered from the Global Navigation Satellite Systems (GNSS) data processing. The purpose of this study is to analyze the differences in GNSS products, such as station coordinates, Earth rotation parameters, geocenter coordinates (GCC), and satellite orbits delivered from the double‐difference multi‐GNSS (GPS, GLONASS, and Galileo) processing, which may arise from (1) using a homogeneous and inhomogeneous network of multi‐GNSS stations, (2) different approaches to the TRF realization using minimum constraint conditions, and (3) different approaches to handling of GCC in GNSS global processing. The questionable quality of GCC delivered from the global GNSS solutions is described with a special attention to network effects and system‐specific parameters. We found that Galileo can provide GCC, whose quality corresponds to the GPS series. Moreover, the GCC from Galileo is of a better quality than those based on GLONASS data, despite the same number of nominal orbital planes and a much lower number of active satellites. When the No‐Net‐Translation constraint is not applied on the GNSS network, the station coordinate repeatability is worsened by about 70%, 55%, and 25% for the north, east, and up components, respectively, compared to the solution when applying No‐Net‐Translation and when having the network origin consistent with the international TRF. We thus infer that the No‐Net‐Translation condition is mandatory in global GNSS solutions.
Oxygen therapy in premature low birth weight infants is associated with capillary loss and increases in blood pressure: a pilot study
Authors: Raghuraman Rajendra P. , Duffy Donovan , Carroll Veronica A. , Manyonda Isaac , Antonios Tarek F.
Published: May 2019

Low birth weight (LBW) and premature birth are known risk factors for future cardiovascular disease and in particular essential hypertension (EH). Capillary rarefaction (CR) is an established hallmark of EH and is known to occur in individuals with a history of LBW. We previously reported that LBW infants do not have CR at birth but rather increased capillary density (CD). We hypothesized that LBW infants undergo a process of accelerated CR in early life, triggered in part by oxygen therapy. We studied 26 LBW infants, of whom 10 infants received oxygen therapy, and compared them to 14 normal birth weight (NBW) infants. We measured CD at 1, 5 and 10 days after birth and again after 40 weeks adjusted gestational age equivalent to birth at full term. We confirmed that LBW infants had higher CD at birth compared to NBW infants and found that significant structural CR occurred at term age in LBW infants who had received oxygen therapy (mean difference −22 capillaries/field, p = 0.007) and in those who did not receive oxygen therapy (mean difference −29 capillaries/field, p < 0.001) compared to baseline at birth. Both LBW groups showed a significant rise in BP at 40 weeks adjusted term age and the rise in systolic (mean difference 24 mm Hg, p < 0.0001) and diastolic BP (mean difference 14 mm Hg, p < 0.001) was more pronounced in the oxygen treated group compared to the nonoxygen group (mean difference 14 mm Hg, p = 0.043 and mean difference = 9 mm Hg p = 0.056 respectively). In conclusion, oxygen therapy in premature LBW infants may induce significant increases in their BP in early life.
Structural basis for the acetylation of histone H3K9 and H3K27 mediated by the histone chaperone Vps75 in Pneumocystis carinii
Authors: Chen Yiping , Zhang Yang , Ye Hui , Dou Yanshu , Lu Deren , Li Xiaolu , Limper Andrew H. , Han Junhong , Su Dan
Published: May 2019

Rtt109 is a histone acetyltransferase (HAT) that is a potential therapeutic target in conditioned pathogenic fungiPneumocystis carinii (P. carinii). The histone chaperone Vps75 can stimulate the Rtt109-dependent acetylation of several histone H3 lysines and preferentially acetylates H3K9 and H3K27 within canonical histone (H3–H4)2tetramers. Vps75 shows two protein conformations assembled into dimeric and tetrameric forms, but the roles played by multimeric forms of Vps75 in Rtt109-mediated histone acetylation remain elusive. InP. carinii, we identified that Vps75 (PcVps75) dimers regulate H3K9 and H3K27 acetylation by directly interacting with histone (H3–H4)2tetramers, rather than by forming a Vps75-Rtt109 complex. For PcVps75 tetramers, the major histone-binding surface is buried within a walnut-like structure in the absence of a histone cargo. Based on crystal structures of dimeric and tetrameric forms of PcVps75, as well as HAT assay data, we confirmed that residues 192E, 193D, 194E, 195E, and 196E and the disordered C-terminal tail (residues 224–250) of PcVps75 mediate interactions with histones and are important for the Rtt109 inP. carinii(PcRtt109)-mediated acetylation of H3K9 and H3K27, both in vitro and in yeast cells. Furthermore, expressing PcRtt109 alone or in combination with PcVps75 variants that cannot effectively bind histones could not fully restore cellular growth in the presence of genotoxic agents that block DNA replication owing to the absence of H3K9 and H3K27 acetylation. Together, these data indicate that the interaction between PcVps75 and histone (H3–H4)2tetramers is a critical regulator of the Rtt109-mediated acetylation of H3K9 and H3K27.
Variability in snake skin microbial assemblages across spatial scales and disease states
Authors: Walker Donald M , Leys Jacob E , Grisnik Matthew , Grajal-Puche Alejandro , Murray Christopher M , Allender Matthew C
Published: May 2019

Understanding how biological patterns translate into functional processes across different scales is a central question in ecology. Within a spatial context, extent is used to describe the overall geographic area of a study, whereas grain describes the overall unit of observation. This study aimed to characterize the snake skin microbiota (grain) and to determine host–microbial assemblage–pathogen effects across spatial extents within the Southern United States. The causative agent of snake fungal disease, Ophidiomyces ophiodiicola, is a fungal pathogen threatening snake populations. We hypothesized that the skin microbial assemblage of snakes differs from its surrounding environment, by host species, spatial scale, season, and in the presence of O. ophiodiicola. We collected snake skin swabs, soil samples, and water samples across six states in the Southern United States (macroscale extent), four Tennessee ecoregions (mesoscale extent), and at multiple sites within each Tennessee ecoregion (microscale extent). These samples were subjected to DNA extraction and quantitative PCR to determine the presence/absence of O. ophiodiicola. High-throughput sequencing was also utilized to characterize the microbial communities. We concluded that the snake skin microbial assemblage was partially distinct from environmental microbial communities. Snake host species was strongly predictive of the skin microbiota at macro-, meso-, and microscale spatial extents; however, the effect was variable across geographic space and season. Lastly, the presence of the fungal pathogen O. ophiodiicola is predictive of skin microbial assemblages across macro- and meso-spatial extents, and particular bacterial taxa associate with O. ophiodiicola pathogen load. Our results highlight the importance of scale regarding wildlife host–pathogen–microbial assemblage interactions.
Culturing the ubiquitous freshwater actinobacterial acI lineage by supplying a biochemical ‘helper’ catalase
Authors: Kim Suhyun , Kang Ilnam , Seo Ji-Hui , Cho Jang-Cheon
Published: May 2019

The actinobacterial acI lineage is among the most successful and ubiquitous freshwater bacterioplankton found on all continents, often representing more than half of all microbial cells in the lacustrine environment and constituting multiple ecotypes. However, stably growing pure cultures of the acI lineage have not been established despite various cultivation efforts based on ecological and genomic studies on the lineage, which is in contrast to the ocean from which abundant microorganisms such as Prochlorococcus, Pelagibacter, and Nitrosopumilus have been isolated. Here, we report the first two pure cultures of the acI lineage successfully maintained by supplementing the growth media with catalase. Catalase was critical for stabilizing the growth of acI strains irrespective of the genomic presence of the catalase-peroxidase (katG) gene. The two strains, representing two novel species, displayed differential phenotypes and distinct preferences for reduced sulfurs and carbohydrates, some of which were difficult to predict based on genomic information. Our results suggest that culture of previously uncultured freshwater bacteria can be facilitated by a simple catalase-supplement method and indicate that genome-based metabolic prediction can be complemented by physiological analyses.
KLF5 regulated lncRNA RP1 promotes the growth and metastasis of breast cancer via repressing p27kip1 translation
Authors: Jia Xiaoting , Shi Lejuan , Wang Xiaorong , Luo Liyun , Ling Li , Yin Jiang , Song Ying , Zhang Zhijie , Qiu Ni , Liu Hao , Deng Min , He Zhimin , Li Hongsheng , Zheng Guopei
Published: May 2019

Increasing evidence suggest that lncRNAs (long noncoding RNAs) play important roles in human cancer. Breast cancer is a heterogeneous disease and the potential involvement of lncRNAs in breast cancer remains unexplored. In this study, we characterized a novel lncRNA, RP1-5O6.5 (termed as RP1). We found that RP1 was highly expressed in breast cancer and predicted poor prognosis of breast cancer patients. Gain-of-function and loss-of-function assays showed that RP1 promoted the proliferation and metastasis of breast cancer cells in vitro and in vivo. Mechanistically, RP1 maintained the EMT and stemness states of breast cancer cells via repressing p27kip1 protein expression. RP1 combined with the complex p-4E-BP1/eIF4E to prevent eIF4E from interacting with eIF4G, therefore attenuating the translational efficiency of p27kip1 mRNA. Furthermore, we found that p27kip1 evidently downregulated Snail1 but not ZEB1 to inhibit invasion of breast cancer cells. Kruppel-like factor 5 (KLF5) was positively correlated with RP1 in breast cancer tissues. Moreover, we demonstrated that KLF5 recruited p300 to the RP1 promoter to enhance RP1 expression. Taken together, our findings demonstrated that KLF5-regulated RP1 plays an oncogenic role in breast cancer by suppressing p27kip1, providing support for the clinical investigation of therapeutic approaches focusing on RP1.
Microarray profiling and co-expression network analysis of lncRNAs and mRNAs in ovarian cancer
Authors: Gao Ce , Zhao Di , Zhao Qing , Dong Dandan , Mu Lin , Zhao Xuejun , Guo Man , Xu Aili , Fang Lei , Liu Qian , Che Jianhua
Published: May 2019

Dysregulated long noncoding RNAs (lncRNAs) are involved in the pathogenesis and development of human diseases, such as epithelial ovarian cancer (EOC). In this study, we identified EOC-related lncRNAs and performed lncRNA and mRNA microarray analyses using IOSE80, a normal ovary cell line, and two ovarian carcinoma cell lines (SKOV3 and SKOV3/DDP) to investigate the potential roles of lncRNAs in EOC. lncRNA-HEIH expression in EOC tissues and cell lines was measured by quantitative real-time polymerase chain reaction (qPCR). In addition, we generated a lncRNA–mRNA co-expression network in order to identify lncRNA-expression trends and potential lncRNA target genes. Cell viability, migration, and invasion were determined by Cell Counting Kit-8, transwell assay, and wound-healing assay, respectively, and apoptosis was analyzed by flow cytometry. We identified 3527 differentially expressed lncRNAs upon comparison of the lncRNA profiles from IOSE80 with those of SKOV3 cell lines, with 11 differentially expressed lncRNAs confirmed by qPCR. Both pathway and gene ontology analyses demonstrated the involvement of lncRNAs, especially HEIH and LINC-PINT, in multiple biological processes. Furthermore, in vitro knockdown experiments confirmed that suppression of HEIH expression inhibited EOC cell proliferation. Our findings provide a foundation for further research into the role of these lncRNAs in EOC carcinogenesis and progression.
Asymmetric synthesis of tetrazole and dihydroisoquinoline derivatives by isocyanide-based multicomponent reactions
Authors: Xiong Qian , Dong Shunxi , Chen Yushuang , Liu Xiaohua , Feng Xiaoming
Published: May 2019

Although isocyanide-based multicomponent reactions were proven to be simple, elegant and facile strategies for the synthesis of highly valuable nitrogen-containing heterocycles, their asymmetric versions accessing to optically active nitrogen heterocyclic compounds are rather limited. Here, we illustrate that, relying on the enantioselective addition of simple isocyanides to C=C bonds, several isocyanide-based multicomponent reactions are realized in the presence of a chiral MgII-N,Nʹ-dioxide catalyst. In the reaction among isocyanide, TMSN3, and alkylidene malonate, three- or four-component reactions could be precisely controlled by modulating reaction conditions, supplying two types of enantioenriched tetrazole-derivatives in moderate to high yields. Possible catalytic cycles via a key zwitterionic intermediate, and the vital roles of H2O or excess ligand are provided based on control experiments. Moreover, taking advantage of this zwitterionic intermediate as a 1,3-dipole, an enantioselective dearomative [3+2] annulation reaction of nonactivated isoquinolines is achieved, furnishing chiral 1,2-dihydroisoquinolines in good to excellent results.
Surface oxidation mechanism of a refractory high-entropy alloy
Authors: Osei-Agyemang Eric , Balasubramanian Ganesh
Published: May 2019

High-entropy alloys (HEAs) synthesized using refractory elements are being strongly considered as candidates for high temperature structural applications. The role of compositional changes of HEA surfaces due to oxidation is crucial to sustain the material properties, but a detailed description of the thermodynamic mechanism driving the adsorption of oxygen on such complex surfaces is absent. We examine and explain the reaction process of oxygen on a representative refractory HEA surface using first principles and atomistic thermodynamic models. The HEA surface is highly reactive to oxygen yielding a full monolayer coverage at temperatures between 300 and 1500 K. The preferential adsorption of oxygen to specific sites of the HEA surface is attributed to the electronic configuration of the bonding shells of the constituent surface atoms. On further oxygen addition, the oxygen atoms diffuse into the bulk regions of the alloy. Manipulation of temperature and oxygen pressure reveals that it is difficult to rid the alloy surface of oxygen even at extremely low pressures of 10−9 bar at 2000 K.
The Global Gridded Crop Model Intercomparison phase 1 simulation dataset
Authors: Müller Christoph , Elliott Joshua , Kelly David , Arneth Almut , Balkovic Juraj , Ciais Philippe , Deryng Delphine , Folberth Christian , Hoek Steven , Izaurralde Roberto C. , Jones Curtis D. , Khabarov Nikolay , Lawrence Peter , Liu Wenfeng , Olin Stefan , Pugh Thomas A. M. , Reddy Ashwan , Rosenzweig Cynthia , Ruane Alex C. , Sakurai Gen , Schmid Erwin , Skalsky Rastislav , Wang Xuhui , de Wit Allard , Yang Hong
Published: May 2019

The Global Gridded Crop Model Intercomparison (GGCMI) phase 1 dataset of the Agricultural Model Intercomparison and Improvement Project (AgMIP) provides an unprecedentedly large dataset of crop model simulations covering the global ice-free land surface. The dataset consists of annual data fields at a spatial resolution of 0.5 arc-degree longitude and latitude. Fourteen crop modeling groups provided output for up to 11 historical input datasets spanning 1901 to 2012, and for up to three different management harmonization levels. Each group submitted data for up to 15 different crops and for up to 14 output variables. All simulations were conducted for purely rainfed and near-perfectly irrigated conditions on all land areas irrespective of whether the crop or irrigation system is currently used there. With the publication of the GGCMI phase 1 dataset we aim to promote further analyses and understanding of crop model performance, potential relationships between productivity and environmental impacts, and insights on how to further improve global gridded crop model frameworks. We describe dataset characteristics and individual model setup narratives.
Texture Analysis of Polycrystalline Vaterite Spherulites from Lake Sturgeon Otoliths
Authors: Chakoumakos Bryan C. , Pracheil Brenda M. , Wood R. Seth , Loeppky Alison , Anderson Gary , Koenigs Ryan , Bruch Ronald
Published: May 2019

Fish otoliths, or ear bones, are comprised of the CaCO3 polymorphs (aragonite, calcite and vaterite), which can occur either alone or in combination. The polymorph phase abundance in an otolith depends on, as yet, unexplained genetic and environmental factors. Most fish otoliths are comprised of the densest CaCO3 polymorph, aragonite. Sturgeon otoliths, on the other hand, contain significant amounts of the rare and the structurally enigmatic polymorph, vaterite. Sturgeon otoliths are frequently comprised of agglomerations of small microcrystalline vaterite spherulites (<300 μm in diameter), that range in shape from nearly perfect spheres to oblate spheroids. These spherulites are similar to the synthetic vaterite microspheres employed in laser trapping applications. Vaterite spherulites from both hatchery-reared (juvenile) and wild (adult) Lake Sturgeon exhibit extreme crystallographic texture as evidenced by X-ray diffraction patterns and their reconstructed pole-figures determined here. The vaterite crystallites making up the spherulites have excellent registry in both the axial and equatorial directions. Whether synthesized or natural, the texture manifested in these spherulites suggests that vaterite nucleates and grows similarly in vivo otolith formation as well as from laboratory synthesis. The uniaxial optical character of the vaterite spherulites, confirmed by these diffraction experiments and combined with their large birefringence, makes them well suited for laser trapping applications.
Immunosuppression Reduces Lung Injury Caused by Mycoplasma pneumoniae Infection
Authors: Shi Shuang , Zhang Xiuqing , Zhou Yao , Tang Heng , Zhao Deyu , Liu Feng
Published: May 2019

The underlying mechanisms of Mycoplasma pneumoniae pneumonia (MPP) pathogenesis are not clearly understood. This study aimed to investigate the correlation between immune response and lung injury in MPP. The clinical characteristics of MPP were compared between patients treated with and without immunosuppressive chemotherapy, and demographic, clinical, and laboratory data were compared between patients with severe and mild MPP. To determine the effect of immune response on lung lesions, mouse MPP and immunosuppression models were established by intranasal inoculation of M129 and intraperitoneal injection of cyclophosphamide, respectively. Myeloperoxidase and oxidant–antioxidant enzyme activities were evaluated for mechanism studies. The immunosuppressant group had a lower incidence of MPP and fewer cases of severe MPP than the non-immunosuppressant group. The severe MPP group had a greater incidence of severe immune disorders than the mild MPP group. Relative to immunosuppressed mice, wild mice exhibited more severe inflammatory infiltration and lung injury as well as a significant increase in myeloperoxidase and malondialdehyde levels and a decrease in superoxide dismutase level after MP infection. In conclusion, immunological responses likely play a vital role in MPP pathogenesis. Lung injury occurring after MP infection—which might be caused by oxidant–antioxidant imbalance—can be reduced by immunosuppression.
Paths, negative “probabilities”, and the Leggett-Garg inequalities
Authors: Sokolovski D. , Gurvitz S. A.
Published: May 2019

We present a path analysis of the condition under which the outcomes of previous observation affect the results of the measurements yet to be made. It is shown that this effect, also known as “signalling in time”, occurs whenever the earlier measurements are set to destroy interference between two or more virtual paths. We also demonstrate that Feynman’s negative “probabilities” provide for a more reliable witness of “signalling in time”, than the Leggett-Garg inequalities, while both methods are frequently subject to failure.
Circulating tumor cells undergoing EMT are poorly correlated with clinical stages or predictive of recurrence in hepatocellular carcinoma
Authors: Chen Yunyang , Li Shaoming , Li Wei , Yang Rongbing , Zhang Xianguang , Ye Yong , Yu Jiexiong , Ye Lin , Tang Wangrong
Published: May 2019

Experimental and clinical studies have highlighted that circulating tumor cell (CTC) with phenotypic hallmarks of epithelial-mesenchymal transition (EMT) plays a critical role in the metastatic and recurrence of solid malignancy. Here we retrospectively evaluated the presence of CTC and its EMT phenotypes in hepatocellular carcinoma (HCC) patients and investigated their clinical relevance. We optimized the CanpatrolTM CTC analysis system to enumerate CTC and classify EMT phenotypes in 113 HCC patients before curative treatment and 143 HCC patients after curative treatment. The relationships between CTC and clinical characteristics were statistically analyzed. None of total CTC or its EMT phenotypes in HCC patients was correlated with clinical characteristics, such as age, sex, HBsAg, Child-Pugh score, liver cirrhosis, AFP, number of tumors, tumor size, vascular invasion and BCLC stage. Neither the level of total CTC nor its EMT phenotypes in HCC patients before or after curative treatment were predictive of recurrence. Additionally, dynamic changes of CTC and its EMT phenotypes were not relevant to HCC recurrence after curative treatment in our study. Wefound CTC count and EMT classification were not correlated with clinical stages or predictive of HCC recurrence, but further large, multicenter studies are needed to confirm these results.
LG biplot: a graphical method for mega-environment investigation using existing crop variety trial data
Authors: Yan Weikai
Published: May 2019

Due to the presence of genotype by environment interaction (GE), no crop cultivar performed the best in all regions. Therefore, the growing regions of a crop must be divided into sub-regions or mega-environments, and specifically adapted cultivars must be bred and deployed in each mega-environment. Meaningful mega-environment delineation must be based on repeatable GE patterns, which can be extracted from multi-year, multi-location crop variety trials. In regional crop variety trials, usually the same set of genotypes are tested across locations within a year, but different sets of genotypes are tested in different years, leading to highly unbalanced multi-year data. Such data are abundant for all crops and regions; but there has been no way to fully utilize them for mega-environment delineation. This paper presents a new method that allows utilization of existing variety trial data to identify repeatable GE patterns, to delineate mega-environments, and to understand the scope of unrepeatable GE at a location and within a mega-environment.
Sinkhole susceptibility mapping in Marion County, Florida: Evaluation and comparison between analytical hierarchy process and logistic regression based approaches
Authors: Subedi Praveen , Subedi Kabiraj , Thapa Bina , Subedi Pradeep
Published: May 2019

Sinkholes are the major cause of concern in Florida for their direct role on aquifer vulnerability and potential loss of lives and property. Mapping sinkhole susceptibility is critical to mitigating these consequences by adopting strategic changes to land use practices. We compared the analytical hierarchy process (AHP) based and logistic regression (LR) based approaches to map the areas prone to sinkhole activity in Marion County, Florida by using long-term sinkhole incident report dataset. For this study, the LR based model was more accurate with an area under the receiver operating characteristic (ROC) curve of 0.8 compared to 0.73 with the AHP based model. Both models performed better when an independent future sinkhole dataset was used for validation. The LR based approach showed a low presence of sinkholes in the very low susceptibility class and low absence of sinkholes in the very high susceptibility class. However, the AHP based model detected sinkhole presence by allocating more area to the high and very high susceptibility classes. For instance, areas susceptible to very high and high sinkhole incidents covered almost 43.4% of the total area under the AHP based approach, whereas the LR based approach allocated 20.7% of the total area to high and very high susceptibility classes. Of the predisposing factors studied, the LR method revealed that closeness to topographic depression was the most important factor for sinkhole susceptibility. Both models classified Ocala city, a populous city of the study area, as being very vulnerable to sinkhole hazard. Using a common test case scenario, this study discusses the applicability and potential limitations of these sinkhole susceptibility mapping approaches in central Florida.
Dynamic magnetic resonance scattering
Authors: Herold Volker , Kampf Thomas , Jakob Peter Michael
Published: May 2019

Dynamic light scattering is a popular technique to determine the size distribution of small particles in the sub micrometer region. It operates in reciprocal space, by analyzing the signal fluctuations with the photon auto correlation function. Equally, pulsed field gradient magnetic resonance is a technique generating data in the reciprocal space of the density distribution of an object. Here we show the feasibility of employing a magnetic resonance imaging system as a dynamic scattering device similar to dynamic light scattering appliances. By acquiring a time series of single data points from reciprocal space, analogue to dynamic light scattering, we demonstrate the examination of motion patterns of microscopic particles. This method allows the examination of particle dynamics significantly below the spatial resolution of magnetic resonance imaging. It is not limited by relaxation times and covers a wide field of applications for particle or cell motion in opaque media.
The catalytic asymmetric synthesis of CF3-containing spiro-oxindole–pyrrolidine–pyrazolone compounds through squaramide-catalyzed 1,3-dipolar cycloaddition
Authors: Wang Cui , Wen Dongwa , Chen Hui , Deng Yabo , Liu Xueting , Liu Xin , Wang Li , Gao Fengyun , Guo Yifei , Sun Mengmeng , Wang Kairong , Yan Wenjin
Published: May 2019

Pharmaceutically important compounds were synthesized through the organocatalytic 1,3-dipolar cycloaddition reaction.
Uniting laboratory automation, DoE data, and modeling techniques to accelerate chemical process development
Authors: Rosso Victor , Albrecht Jacob , Roberts Frederick , Janey Jacob M.
Published: May 2019

Rapid knowledge building of chemical processes with highly automated DoE (HAD) and statistical analyses and modeling.
Polyethylenimine-modified nickel phosphide nanosheets: interfacial protons boost the hydrogen evolution reaction
Authors: Ding Yu , Miao Bo-Qiang , Jiang Yu-Cheng , Yao Hong-Chang , Li Xi-Fei , Chen Yu
Published: May 2019

The hydrogen evolution reaction (HER) is an important half-reaction in water electrolysis. According to the Nernst equation, increasing the interfacial proton concentration may be an effective strategy for reducing the overpotential of HER.
Improved tumor targeting and penetration by a dual-functional poly(amidoamine) dendrimer for the therapy of triple-negative breast cancer
Authors: Liu Changliang , Gao Houqian , Zhao Zijian , Rostami Iman , Wang Chen , Zhu Ling , Yang Yanlian
Published: May 2019

A dual-functional drug delivery system based on the conjugation of PAMAM dendrimer with EBP-1 and TAT peptide was established for the therapy of triple-negative breast cancer.