bluuebunny/crossref_metadata_2025 huggingface dataset:
An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota
Authors: Muggia Lucia , Ametrano Claudio G. , Sterflinger Katja , Tesei Donatella
Published: December 2020

Fungi are among the most successful eukaryotes on Earth: they have evolved strategies to survive in the most diverse environments and stressful conditions and have been selected and exploited for multiple aims by humans. The characteristic features intrinsic of Fungi have required evolutionary changes and adaptations at deep molecular levels. Omics approaches, nowadays including genomics, metagenomics, phylogenomics, transcriptomics, metabolomics, and proteomics have enormously advanced the way to understand fungal diversity at diverse taxonomic levels, under changeable conditions and in still under-investigated environments. These approaches can be applied both on environmental communities and on individual organisms, either in nature or in axenic culture and have led the traditional morphology-based fungal systematic to increasingly implement molecular-based approaches. The advent of next-generation sequencing technologies was key to boost advances in fungal genomics and proteomics research. Much effort has also been directed towards the development of methodologies for optimal genomic DNA and protein extraction and separation. To date, the amount of proteomics investigations in Ascomycetes exceeds those carried out in any other fungal group. This is primarily due to the preponderance of their involvement in plant and animal diseases and multiple industrial applications, and therefore the need to understand the biological basis of the infectious process to develop mechanisms for biologic control, as well as to detect key proteins with roles in stress survival. Here we chose to present an overview as much comprehensive as possible of the major advances, mainly of the past decade, in the fields of genomics (including phylogenomics) and proteomics of Ascomycota, focusing particularly on those reporting on opportunistic pathogenic, extremophilic, polyextremotolerant and lichenized fungi. We also present a review of the mostly used genome sequencing technologies and methods for DNA sequence and protein analyses applied so far for fungi.
Electromagnetic Shielding Performance of Different Metallic Coatings Deposited by Arc Thermal Spray Process
Authors: Jang Jong-Min , Lee Han-Seung , Singh Jitendra Kumar
Published: December 2020

Advancement in electronic and communication technologies bring us up to date, but it causes electromagnetic interference (EMI) resulting in failure of building and infrastructure, hospital, military base, nuclear plant, and sensitive electronics. Therefore, it is of the utmost importance to prevent the failure of structures and electronic components from EMI using conducting coating. In the present study, Cu, Cu-Zn, and Cu-Ni coating was deposited in different thicknesses and their morphology, composition, conductivity, and EMI shielding effectiveness are assessed. The scanning electron microscopy (SEM) results show that 100 µm coating possesses severe defects and porosity but once the thickness is increased to 500 µm, the porosity and electrical conductivity is gradually decreased and increased, respectively. Cu-Zn coating exhibited lowest in porosity, dense, and compact morphology. As the thickness of coating is increased, the EMI shielding effectiveness is increased. Moreover, 100 µm Cu-Zn coating shows 80 dB EMI shielding effectiveness at 1 GHz but Cu and Cu-Ni are found to be 68 and 12 dB, respectively. EMI shielding effectiveness results reveal that 100 µm Cu-Zn coating satisfy the minimum requirement for EMI shielding while Cu and Cu-Ni required higher thickness.
Eco-UHPC as Repair Material—Bond Strength, Interfacial Transition Zone and Effects of Formwork Type
Authors: Kothari Ankit , Rajczakowska Magdalena , Buasiri Thanyarat , Habermehl-Cwirzen Karin , Cwirzen Andrzej
Published: December 2020

A reduced carbon footprint and longer service life of structures are major aspects of circular economy with respect to civil engineering. The aim of the research was to evaluate the interfacial bond properties between a deteriorated normal strength concrete structure and a thin overlay made of Eco-UHPC containing 50 wt% of limestone filler. Two types of formwork were used: untreated rough plywood and surface treated shuttering plywood. The normal strength concrete elements were surface scaled using water jets to obtain some degradation prior to casting of the UHPC overlay. Ultrasonic pulse velocity (UPV), bond test (pull-off test), and Scanning Electron Microscopy (SEM) combined with Energy Dispersive Spectrometry (EDS) were used for analysis. Elements repaired with the Eco-UHPC showed significantly improved mechanical properties compared to the non-deteriorated NSC sample which was used as a reference. The bond strength varied between 2 and 2.7 MPa regardless of the used formwork. The interfacial transition zone was very narrow with only slightly increased porosity. The untreated plywood, having a rough and water-absorbing surface, created a surface friction-based restraint which limited microcracking due to autogenous shrinkage. Shuttering plywood with a smooth surface enabled the development of higher tensile stress on the UHPC surface, which led to a more intensive autogenous shrinkage cracking. None of the formed microcracks penetrated through the entire thickness of the overlay and some were partly self-healed when a simple water treatment was applied. The project results showed that application of UHPC as repair material for concrete structures could elongate the lifespan and thus enhance the sustainability.
An Aqueous Exfoliation of WO3 as a Route for Counterions Fabrication—Improved Photocatalytic and Capacitive Properties of Polyaniline/WO3Composite
Authors: Szkoda Mariusz , Zarach Zuzanna , Trzciński Konrad , Nowak Andrzej P.
Published: December 2020

In this paper, we demonstrate a novel, electrochemical route of polyaniline/tungsten oxide (PANI)/WO3) film preparation. Polyaniline composite film was electrodeposited on the FTO (fluorine-doped tin oxide) substrate from the aqueous electrolyte that contained aniline (monomer) and exfoliated WO3 as a source of counter ions. The chemical nature of WO3 incorporated in the polyaniline matrix was investigated using X-ray photoelectron spectroscopy. SEM (scanning electron microscopy) showed the impact of WO3 presence on the morphology of polyaniline film. PANI/WO3 film was tested as an electrode material in an acidic electrolyte. Performed measurements showed the electroactivity of both components and enhanced electrochemical stability of PANI/WO3 in comparison with PANI/Cl. Thus, PANI/WO3 electrodes were utilized to construct the symmetric supercapacitors. The impact of capacitive and diffusion-controlled processes on the mechanism of electrical energy storage was quantitatively determined. Devices exhibited high electrochemical capacity of 135 mF cm−2 (180 F g−1) and satisfactory retention rate of 70% after 10,000 cycles. The electrochemical energy storage device exhibited 1075.6 W kg−1 of power density and 12.25 Wh kg−1 of energy density. We also investigated the photocatalytic performance of the deposited film. Photodegradation efficiencies of methylene blue and methyl orange using PANI/WO3 and PANI/Cl were compared. The mechanism of dye degradation using WO3-containing films was investigated in the presence of scavengers. Significantly higher efficiency of photodecomposition of dyes was achieved for composite films (84% and 86%) in comparison with PANI/Cl (32% and 39%) for methylene blue and methyl orange, respectively.
Textile Reinforced Concrete in Combination with Improved Self-Healing Ability Caused by Crystalline Admixture
Authors: Žáková Hana , Pazderka Jiří , Reiterman Pavel
Published: December 2020

The main aim of this study was to investigate the improved autogenous healing of concrete caused by a crystalline admixture in combination with textile reinforced concrete (TRC). This phenomenon (improved healing) has not yet been described by any independent study, and not at all in relation to TRC. The results of the study confirmed that the interaction between TRC and the crystalline admixture’s self-healing ability is advantageous and usable. The application of crystalline admixture could ensure the long-term entirety of the TCR element, where microcracks could occur. This allows for the creation of advantageous, thin (achieved by TRC) and waterproof (achieved by the crystalline admixtures) concrete structures. Moreover, this does not depend on temperature in the range of 4–30 °C (lower temperatures are of course problematic, as for most other cementitious materials). However, the interaction of both materials has its limits; the cracks must not be too wide (max. 0.1 mm), otherwise they will not heal. On the other hand, the advantage is that it does not matter what type of cement is used (CEM I and CEM II showed the same results), and the composition of the newly formed crystals in the cracks corresponds to the composition of the C-S-H gel, so it can be assumed that secondary hydration of the Portland cement occurred in the crack area.
The Influence of Sub-Zero Conditions on the Mechanical Properties of Polylactide-Based Composites
Authors: Mysiukiewicz Olga , Barczewski Mateusz , Kloziński Arkadiusz
Published: December 2020

Polylactide-based composites filled with waste fillers due to their sustainability are a subject of many current papers, in which their structural, mechanical, and thermal properties are evaluated. However, few studies focus on their behavior in low temperatures. In this paper, dynamic and quasi-static mechanical properties of polylactide-based composites filled with 10 wt% of linseed cake (a by-product of mechanical oil extraction from linseed) were evaluated at room temperature and at −40 °C by means of dynamic mechanical analysis (DMA), Charpy’s impact strength test and uniaxial tensile test. It was found that the effect of plasticization provided by the oil contained in the filler at room temperature is significantly reduced in sub-zero conditions due to solidification of the oil around −18 °C, as it was shown by differential scanning calorimetry (DSC) and DMA, but the overall mechanical performance of the polylactide-based composites was sufficient to enable their use in low-temperature applications.
Improving the Performance of a Graphite Foil/Polyaniline Electrode Material by a Thin PEDOT:PSS Layer for Application in Flexible, High Power Supercapacitors
Authors: Zarach Zuzanna , Trzciński Konrad , Łapiński Marcin , Lisowska-Oleksiak Anna , Szkoda Mariusz
Published: December 2020

In this study, we present a novel strategy for enhancing polyaniline stability and thus obtaining an electrode material with practical application in supercapacitors. A promising (graphite foil/polyaniline/poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) GF/PANI/PEDOT:PSS) electrode material was characterized and used in the construction of a symmetric supercapacitor that provides an outstanding high power density. For this purpose, the electropolymerization of PANI was carried out on a graphite foil and then a thin protective layer of PEDOT:PSS was deposited. The presence of the nanometer PEDOT:PSS layer made it possible to widen the electroactivity potential range of the electrode material. Moreover, the synergy between materials positively affected the amount of accumulated charge, and thus the thin PEDOT:PSS layer contributed to enhancing the specific capacity of the electrode material. The electrochemical performance of the GF/PANI/PEDOT:PSS electrode, as well as the symmetrical supercapacitor, was investigated by cyclic voltammetry and galvanostatic charge/discharge cycles in 1 M H2SO4 at room temperature. The fabricated electrode material shows a high specific capacitance (Csp) of 557.4 Fg−1 and areal capacitance (Careal) of 2600 mF·cm−2 in 1 M H2SO4 at a current density of 200 mA·cm−2 (~4 A·g−1). The supercapacitor performance was studied and the results show that a thin PEDOT:PSS layer enables cycling stability improvement of the device from 54% to 67% after 10,000 cycles, and provides a high specific capacity (159.8 F·g−1) and a maximum specific power (18,043 W·kg−1) for practical applications.
Influence of the Interlayer Temperature on Structure and Properties of Wire and Arc Additive Manufactured Duplex Stainless Steel Product
Authors: Knezović Nikola , Garašić Ivica , Jurić Ivan
Published: December 2020

WAAM (wire and arc additive manufacturing) is becoming an increasingly popular method to produce components from metals, which are usually not so suitable for conventional production methods. One of the good examples is duplex stainless steels (DSSs), which are quite complex for welding and machining. Excessive ferrite amount is a common problem for them and controlling an interlayer temperature could offer a solution. However, using too low interlayer temperature will slow down the whole process and compromise one of the WAAM’s main advantages—the high productivity. The aim of this study is to find the relationship between interlayer temperature and process duration and to determine the influence of the interlayer temperature on product structure and other properties. Three samples (walls) were made using different interlayer temperatures (50 °C, 100 °C and 150 °C) and they were tested to analyze their surface texture, chemical composition, ferrite amount, the appearance of porosity and the hardness. Ferrite amount was higher and there was more porosity on lower interlayer temperatures, while there is no significant difference between surface texture and chemical composition for the samples. Considering the fact that higher interlayer temperatures provide a faster process, they should be preferred to produce duplex stainless steel products.
The Role of Observation–Measurement Methods in the Surface Characterization of X39Cr13 Stainless-Steel Cutting Blades Used in the Fish Processing Industry
Authors: Kapłonek Wojciech , Nadolny Krzysztof , Zieliński Bartosz , Plichta Jarosław , Pimenov Danil , Sharma Shubham
Published: December 2020

In the modern fish processing industry, flat fishes play an important role. They are processed into a final product in the form of a fillet during the skinning operation, which is carried out on machines operating in automated production lines. These machines are usually equipped with a single planar cutting blade or a few of such blades. The high-efficiency skinning and industrial conditions cause rapid wear of the cutting edge of the blade, which is detrimental to the quality of the final product. One of the forms of renewing the cutting ability of these types of tools is the regeneration carried out with the use of precise traverse surface grinding. The results of this process must be carefully verified for determining its correctness and possible optimization of its parameters. The main goal of this article was to characterize the usefulness of a number of observational and measuring methods to evaluate the results of the technical blade regeneration process. In this work, a number of contemporary observation–measurement methods such as optical microscopy (OM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), optical profilometry (OP), and angle-resolved scattering (ARS), supported by image processing and analysis techniques, were analyzed. The authors focused on presenting the role of the abovementioned methods in the surface characterization of planar cutting blades made of X39Cr13 chromium martensitic stainless steel before and after the technological operation of flat-fish skinning. Additionally, the surface condition after the regeneration process carried out using the five-axis CNC (computerized numerical control) grinding machine was also assessed. Numerous results of surface observations, elemental composition microanalysis, high-accuracy surface microgeometry measurements, and quantitative and qualitative analysis confirming the possibility of using the proposed methods in the presented applications are presented.
Tribological Properties of Ni-P/Si3N4 Nanocomposite Layers Deposited by Chemical Reduction Method on Aluminum Alloy AW-7075
Authors: Czapczyk Kazimierz
Published: December 2020

The article presents the results of tribological tests of Ni-P/Si3N4 nanocomposite and Ni-P nickel layers deposited on the AW-7075 aluminum alloy by chemical reduction method, and the AW-7075 alloy without coating. Nanocomposite layers were produced using Si3N4 siliconnitride in the form of a polydisperse powder whose particle sizes ranged from 20 to 25 nm. The influence of the content of the dispersion phase layer material on the abrasive wear, which was determined as the “ball on disc” method, was analyzed. Surface topography was examined by the contact method using a profilometer. The purpose of introducing Si3N4 particles into the Ni-P layer was to increase the wear resistance of AW-7075 aluminum alloy parts with an embedded nanocomposite coating. Based on the obtained test results, it was found that the Ni-P/Si3N4 layers are more resistant to wear than the Ni-P layers and the AW-7075 alloy layers, and are a good barrier against abrasive wear at various loads and environmental conditions.
Numerical Analysis of the Heating Characteristics of Magnetic Oscillation Arc and the Fluid Flow in Molten Pool in Narrow Gap Gas Tungsten Arc Welding
Authors: Jian Xiaoxia , Yang Xing , Li Jingqian , Wang Weihua , Wu Hebao
Published: December 2020

Magnetic oscillation arc (MOA) technology was developed to avoid insufficient fusion defects appearing at the sidewalls in narrow gap gas tungsten arc welding (NG-GTAW). In this work, a unified model was developed to simulate the process of MOA assisted NG-GTAW. The model included the MOA, welding pool, workpiece and the coupling interaction between them. The heating characteristic of the MOA and the flow of liquid metal were simulated, and the mechanism of forming a uniform welding bead under MOA was investigated. It was found that if the magnetic flux density increased to 9 mT, the MOA could point to the sidewall directly; the maximum heat flux at the bottom declined by almost half and at the side, it increased by more than ten times. Additionally, the heat flux was no longer concentrated but dispersed along the narrow groove face. Under the effect of MOA, there were mainly two flow vortexes in the molten pool, which could further increase the heat distribution between the bottom, sidewall and corner, and was beneficial for the formation of a good-shape weld. The model was validated by experimental data.
Shear Performance of Reinforced Concrete Beams with Small Circular Openings Strengthened Using Rectangular and Octagonal-Shaped Reinforcement
Authors: Kim Hyeong-Gook , Lee Jung-Yoon , Kim Kil-Hee
Published: December 2020

An experimental case study was carried out to investigate the shear performance of reinforced concrete beams with small circular openings under a cyclic anti-symmetric bending moment. The openings were strengthened by using a newly developed reinforcement continuously bent into rectangular and octagonal shapes, which was convenient for installation and effective for crack control. The presence of web opening reinforcement, the reinforcing method, and the web opening spacing were employed as main variables in the design of five specimens. The cyclic performance of all specimens was evaluated in terms of failure mode, crack pattern, strength and stiffness degradation, and strain distribution. Experimental results were discussed to assess the suitability of the proposed web opening reinforcement in RC web opening beams. It was confirmed that the proposed web opening reinforcement exhibited outstanding crack control and served as a shear resistance component in place of the concrete cross-section lost due to web openings. Finally, the shear strength of all specimens, obtained from the cyclic loading tests, were compared with those obtained from the equation proposed by Mansur (1998) and the Architectural Institute of Japan standard 2010.
Heteroexpression of Aspergillus nidulans laeA in Marine-Derived Fungi Triggers Upregulation of Secondary Metabolite Biosynthetic Genes
Authors: Khan Ishrat , Xie Wan-Lin , Yu Yu-Chao , Sheng Huan , Xu Yan , Wang Jia-Qi , Debnath Sanjit Chandra , Xu Jin-Zhong , Zheng Dao-Qiong , Ding Wan-Jing , Wang Pin-Mei
Published: December 2020

Fungi are a prospective resource of bioactive compounds, but conventional methods of drug discovery are not effective enough to fully explore their metabolic potential. This study aimed to develop an easily attainable method to elicit the metabolic potential of fungi using Aspergillus nidulans laeA as a transcription regulation tool. In this study, functional analysis of Aspergillus nidulans laeA (AnLaeA) and Aspergillus sp. Z5 laeA (Az5LaeA) was done in the fungus Aspergillus sp. Z5. Heterologous AnLaeA-and native Az5LaeA-overexpression exhibited similar phenotypic effects and caused an increase in production of a bioactive compound diorcinol in Aspergillus sp. Z5, which proved the conserved function of this global regulator. In particular, heteroexpression of AnLaeA showed a significant impact on the expression of velvet complex genes, diorcinol synthesis-related genes, and different transcription factors (TFs). Moreover, heteroexpression of AnLaeA influenced the whole genome gene expression of Aspergillus sp. Z5 and triggered the upregulation of many genes. Overall, these findings suggest that heteroexpression of AnLaeA in fungi serves as a simple and easy method to explore their metabolic potential. In relation to this, AnLaeA was overexpressed in the fungus Penicillium sp. LC1-4, which resulted in increased production of quinolactacin A.
Extraction Improvement of the Bioactive Blue-Green Pigment “Marennine” from Diatom Haslea ostrearia’s Blue Water: A Solid-Phase Method Based on Graphitic Matrices
Authors: Bélanger William , Arnold Alexandre A. , Turcotte François , Saint-Louis Richard , Deschênes Jean-Sébastien , Genard Bertrand , Marcotte Isabelle , Tremblay Réjean
Published: December 2020

The compound “marennine” is a blue–green pigment produced by the benthic microalgae Haslea ostrearia, with pathogenicity reduction activities against some bacteria and promising potential as a natural pigment in seafood industries. After decades of research, the chemical family of this compound still remains unclear, mainly because structural studies were impaired by the presence of co-extracted compounds in marennine isolates. To improve the purity of marennine extract, we developed a novel extraction method using a graphitic stationary phase, which provides various advantages over the previous procedure using tandem ultrafiltration. Our method is faster, more versatile, provides a better crude yield (66%, compared to 57% for ultrafiltration) and is amenable to upscaling with continuous photobioreactor cultivation. Our goal was to take advantage of the modulable surface properties of the graphitic matrix by optimizing its interactions with marennine. As such, the effects of organic modifiers, pH and reducing agents were studied. With this improvement on marennine purification, we achieved altogether the isolation of a fucoidan-related, sulfated polysaccharide from blue water. Characterization of the polysaccharides fraction suggests that roughly half of UV-absorbing compounds could be isolated from the marennine crude extracts. The identification of sulfated polysaccharides could be a major breakthrough for marennine purification, providing targeted isolation techniques. Likewise, the added value of Haslea ostrearia and the role of polysaccharides in previous marennine chemical characterization and bioactivity studies remain to be determined.
The Second Wave of COVID-19 Pandemic Strikes during the Flu Season: An Awareness Perspective
Authors: Burlacu Alexandru , Crisan-Dabija Radu , Popa Iolanda Valentina , Covic Adrian
Published: December 2020

Coinfection with both SARS-CoV-2 and influenza viruses seems to be a real and severe problem. However, coinfection is far from a simple matter, and cannot be considered having more unfavorable outcomes as a direct consequence. In reality, the aftermath is powerfully nuanced by the presence of risk factors and specific molecular mechanisms. Our objective was to raise awareness around the unpredictable association between COVID-19 pandemics and the upcoming flu season, and make arguments about the need to develop new routine testing protocols for both viruses, at least during the period with an expected high incidence. Our reasoning is built around the various impacts that the whole range of risk groups, common immunological mechanisms, and complex interactions, such as influenza vaccination, will have on patients’ prognosis. We show that the more flawed clinical course is due to managing only one of the infections (and, subsequently, neglecting the other condition).
Performance Evaluation and Fouling Propensity of Forward Osmosis (FO) Membrane for Reuse of Spent Dialysate
Authors: Kim Chaeyeon , Lee Chulmin , Kim Soo Wan , Kim Chang Seong , Kim In S.
Published: December 2020

The number of chronic renal disease patients has shown a significant increase in recent decades over the globe. Hemodialysis is the most commonly used treatment for renal replacement therapy (RRT) and dominates the global dialysis market. As one of the most water-consuming treatments in medical procedures, hemodialysis has room for improvement in reducing wastewater effluent. In this study, we investigated the technological feasibility of introducing the forward osmosis (FO) process for spent dialysate reuse. A 30 LMH of average water flux has been achieved using a commercial TFC membrane with high water permeability and salt removal. The water flux increased up to 23% with increasing flowrate from 100 mL/min to 500 mL/min. During 1 h spent dialysate treatment, the active layer facing feed solution (AL-FS) mode showed relatively higher flux stability with a 4–6 LMH of water flux reduction while the water flux decreased significantly at the active layer facing draw solution (AL-DS) mode with a 10–12 LMH reduction. In the pressure-assisted forward osmosis (PAFO) condition, high reverse salt flux was observed due to membrane deformation. During the membrane filtration process, scaling occurred due to the influence of polyvalent ions remaining on the membrane surface. Membrane fouling exacerbated the flux and was mainly caused by organic substances such as urea and creatinine. The results of this experiment provide an important basis for future research as a preliminary experiment for the introduction of the FO technique to hemodialysis.
A New 3D Printing System of Poly(3,4-ethylenedioxythiophene) for Realizing a High Electrical Conductivity and Fine Processing Resolution
Authors: Yamada Katsumi , Sone Junji
Published: December 2020

Micro-nano 3D printing of the conductive 3,4-ethylenedioxythiophene polymer (PEDOT) was performed in this study. An oil immersion objective lens was introduced into the 3D photofabrication system using a femtosecond pulsed laser as the light source. As a result, the processing resolution in the horizontal and vertical directions was improved in comparison to our previous study. A relatively high electrical conductivity (3500 S/cm) was found from the obtained 3D PEDOT micro-structures. It is noteworthy that the high conductivity of the PEDOT was obtained in the mixed state with an insulating Nafion sheet.
Numerical Study of T-Shaped Micromixers with Vortex-Inducing Obstacles in the Inlet Channels
Authors: Wu Chih-Yang , Lai Bing-Hao
Published: December 2020

To enhance fluid mixing, a new approach for inlet flow modification by adding vortex-inducing obstacles (VIOs) in the inlet channels of a T-shaped micromixer is proposed and investigated in this work. We use a commercial computational fluid dynamics code to calculate the pressure and the velocity vectors and, to reduce the numerical diffusion in high-Peclet-number flows, we employ the particle-tracking simulation with an approximation diffusion model to calculate the concentration distribution in the micromixers. The effects of geometric parameters, including the distance between the obstacles and the angle of attack of the obstacles, on the mixing performance of micromixers are studied. From the results, we can observe the following trends: (i) the stretched contact surface between different fluids caused by antisymmetric VIOs happens for the cases with the Reynolds number (Re) greater than or equal to 27 and the enhancement of mixing increases with the increase of Reynolds number gradually, and (ii) the onset of the engulfment flow happens at Re≈125 in the T-shaped mixer with symmetric VIOs or at Re≈140 in the standard planar T-shaped mixer and results in a sudden increase of the degree of mixing. The results indicate that the early initiation of transversal convection by either symmetric or antisymmetric VIOs can enhance fluid mixing at a relatively lower Re.
A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange
Authors: Ito Yoshihisa , Izawa Yusuke , Osaki Toshihisa , Kamiya Koki , Misawa Nobuo , Fujii Satoshi , Mimura Hisatoshi , Miki Norihisa , Takeuchi Shoji
Published: December 2020

Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurements using a pumpless solution exchange procedure. The device consists of a millimeter-scale cylindrical cup with vertical slits which is designed to steadily hold an aqueous solution and exchange the sample by simply fusing and splitting the solution with an external solution. The slit design was experimentally determined by the capabilities of both the retention and exchange of the solution. Using the optimized slit, a planar lipid bilayer was reconstituted with a nanopore protein at a microaperture allocated to the bottom of the cup, and the device was connected to a portable amplifier. The solution exchangeability was demonstrated by observing the dilution process of a blocker molecule of the nanopore dissolved in the cup. The pumpless solution exchange by the proposed cup-like device presents potential as a lipid-bilayer system for portable sensing applications.
Thermoelectrically-Cooled InAs/GaSb Type-II Superlattice Detectors as an Alternative to HgCdTe in a Real-Time Mid-Infrared Backscattering Spectroscopy System
Authors: Müller Raphael , Haertelt Marko , Niemasz Jasmin , Schwarz Klaus , Daumer Volker , Flores Yuri V. , Ostendorf Ralf , Rehm Robert
Published: December 2020

We report on the development of thermoelectrically cooled (TE-cooled) InAs/GaSb type-II superlattice (T2SL) single element infrared (IR) photodetectors and exemplify their applicability for real-time IR spectroscopy in the mid-infrared in a possible application. As the European Union’s Restriction of Hazardous Substances (RoHS) threatens the usage of the state-of-the-art detector material mercury cadmium telluride (MCT), RoHS-compatible alternatives to MCT have to be established for IR detection. We use bandgap engineered InAs/GaSb T2SLs to tailor the temperature-dependent bandgap energy for detection throughout the required spectral range. Molecular beam epitaxy of superlattice samples is performed on GaAs substrates with a metamorphic GaAsSb buffer layer. Photolithographic processing yields laterally-operated T2SL photodetectors. Integrated in a TE-cooled IR detector module, such T2SL photodetectors can be an alternative to MCT photodetectors for spectroscopy applications. Here, we exemplify this by exchanging a commercially available MCT-based IR detector module with our T2SL-based IR detector module in a real-time mid-infrared backscattering spectroscopy system for substance identification. The key detector requirements imposed by the spectroscopy system are a MHz-bandwidth, a broad spectral response, and a high signal-to-noise ratio, all of which are covered by the reported T2SL-based IR detector module. Hence, in this paper, we demonstrate the versatility of TE-cooled InAs/GaSb T2SL photodetectors and their applicability in an IR spectroscopy system.
Manufacturability and Stress Issues in 3D Silicon Detector Technology at IMB-CNM
Authors: Quirion David , Manna Maria , Hidalgo Salvador , Pellegrini Giulio
Published: December 2020

This paper provides an overview of 3D detectors fabrication technology developed in the clean room of the Microelectronics Institute of Barcelona (IMB-CNM). Emphasis is put on manufacturability, especially on stress and bow issues. Some of the technological solutions proposed at IMB-CNM to improve manufacturability are presented. Results and solutions from other research institutes are also mentioned. Analogy with through-silicon-via technology is drawn. This article aims at giving hints of the technology improvements implemented to upgrade from a R&D process to a mature technology.
Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery
Authors: Blesken Christian C. , Strümpfler Tessa , Tiso Till , Blank Lars M.
Published: December 2020

The production of biosurfactants is often hampered by excessive foaming in the bioreactor, impacting system scale-up and downstream processing. Foam fractionation was proposed to tackle this challenge by combining in situ product removal with a pre-purification step. In previous studies, foam fractionation was coupled to bioreactor operation, hence it was operated at suboptimal parameters. Here, we use an external fractionation column to decouple biosurfactant production from foam fractionation, enabling continuous surfactant separation, which is especially suited for system scale-up. As a subsequent product recovery step, continuous foam adsorption was integrated into the process. The configuration is evaluated for rhamnolipid (RL) or 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA, i.e., RL precursor) production by recombinant non-pathogenic Pseudomonas putida KT2440. Surfactant concentrations of 7.5 gRL/L and 2.0 gHAA/L were obtained in the fractionated foam. 4.7 g RLs and 2.8 g HAAs could be separated in the 2-stage recovery process within 36 h from a 2 L culture volume. With a culture volume scale-up to 9 L, 16 g RLs were adsorbed, and the space-time yield (STY) increased by 31% to 0.21 gRL/L·h. We demonstrate a well-performing process design for biosurfactant production and recovery as a contribution to a vital bioeconomy.
Hyperspectral Characteristics of Oil Sand, Part 2: Prediction of Froth Characteristics from Measurements of Froth
Authors: Rivard Benoit , Feng Jilu , Russell Derek , Bushan Vivek , Lipsett Michael
Published: December 2020

This is the second part of a study of predictive models of oil sand ore and froth characteristics using infrared hyperspectral data as a potential new means for process control. In Alberta, Canada, bitumen in shallow oil sands deposits is accessed by surface mining and then extracted from ore using flotation processes. The ore displays variability in the clay, bitumen, and fines content and this variability affects the separability and product quality in flotation units. Flotation experiments were performed on a set of ore samples of different types to generate froth and determine the ore processability (e.g., separation performance) and froth characteristics (bitumen and solids content, fines distribution). We show that point spectra and spectral imagery of good quality can be acquired rapidly (<1 s and <15 s, respectively) and these capture spectral features diagnostic of bitumen and solids. Ensuing models can predict the solids/bitumen (r2 = 0.88) and the %fines and ultrafines (particle passing at 3.9 and 0.5 µm) content of froth (r2 = 0.8 and 0.9, respectively). The latter model could be used to reject froth with a high solids content. Alternately, the strength of the illite-smectite absorption observed in froth could be used to retain all the samples above a pre-defined processability. Given that point spectrometers can currently be acquired for less than half the cost of an imaging system, we recommend the use of the former for future trials in operating environments.
Cytotoxicity and Bioimaging Study for NHDF and HeLa Cell Lines by Using Graphene Quantum Pins
Authors: Jeon Seong-Beom , Samal Monica , Govindaraju Saravanan , Ragini Das Rupasree , Yun Kyusik
Published: December 2020

Herein, we report the synthesis of an interesting graphene quantum material called “graphene quantum pins (GQPs)”. Morphological analysis revealed the interesting pin shape (width: ~10 nm, length: 50–100 nm) and spectral analysis elucidated the surface functional groups, structural features, energy levels, and photoluminescence properties (blue emission under 365 nm). The difference between the GQPs and graphene quantum dos (GQDs) isolated from the same reaction mixture as regards to their morphological, structural, and photoluminescence properties are also discussed along with the suggestion of a growth mechanism. Cytotoxicity and cellular responses including changes in biophysical and biomechanical properties were evaluated for possible biomedical applications of GQPs. The studies demonstrated the biocompatibility of GQPs even at a high concentration of 512 μg/mL. Our results suggest GQPs can be used as a potential bio-imaging agent with desired photoluminescence property and low cytotoxicity.
An Organic–Inorganic Hybrid Nanocomposite as a Potential New Biological Agent
Authors: Dulski Mateusz , Malarz Katarzyna , Kuczak Michał , Dudek Karolina , Matus Krzysztof , Sułowicz Sławomir , Mrozek-Wilczkiewicz Anna , Nowak Anna
Published: December 2020

To solve the problem of human diseases caused by a combination of genetic and environmental factors or by microorganisms, intense research to find completely new materials is required. One of the promising systems in this area is the silver-silica nanocomposites and their derivatives. Hence, silver and silver oxide nanoparticles that were homogeneously distributed within a silica carrier were fabricated. Their average size was d = (7.8 ± 0.3) nm. The organic polymers (carboxymethylcellulose (CMC) and sodium alginate (AS)) were added to improve the biological features of the nanocomposite. The first system was prepared as a silver chlorine salt combination that was immersed on a silica carrier with coagulated particles whose size was d = (44.1 ± 2.3) nm, which coexisted with metallic silver. The second system obtained was synergistically interacted metallic and oxidized silver nanoparticles that were distributed on a structurally defective silica network. Their average size was d = (6.6 ± 0.7) nm. Physicochemical and biological experiments showed that the tiny silver nanoparticles in Ag/SiO2 and Ag/SiO2@AS inhibited E. coli, P. aeruginosa, S. aureus, and L. plantarum’s cell growth as well as caused a high anticancer effect. On the other hand, the massive silver nanoparticles of Ag/SiO2@CMC had a weaker antimicrobial effect, although they highly interacted against PANC-1. They also generated reactive oxygen species (ROS) as well as the induction of apoptosis via the p53-independent mechanism.
Organic–Inorganic Ternary Nanohybrids of Single-Walled Carbon Nanohorns for Room Temperature Chemiresistive Ethanol Detection
Authors: Cobianu Cornel , Serban Bogdan-Catalin , Dumbravescu Niculae , Buiu Octavian , Avramescu Viorel , Pachiu Cristina , Bita Bogdan , Bumbac Marius , Nicolescu Cristina-Mihaela , Cobianu Cosmin
Published: December 2020

Organic–inorganic ternary nanohybrids consisting of oxidized-single walled carbon nanohorns-SnO2-polyvinylpyrrolidone (ox-SWCNH/SnO2/PVP) with stoichiometry 1/1/1 and 2/1/1 and ox-SWCNH/ZnO/PVP = 5/2/1 and 5/3/2 (all mass ratios) were synthesized and characterized as sensing films of chemiresistive test structures for ethanol vapor detection in dry air, in the range from 0 up to 50 mg/L. All the sensing films had an ox-SWCNH concentration in the range of 33.3–62.5 wt%. A comparison between the transfer functions and the response and recovery times of these sensing devices has shown that the structures with ox-SWCNH/SnO2/PVP = 1/1/1 have the highest relative sensitivities of 0.0022 (mg/L)−1, while the devices with ox-SWCNH/SnO2/PVP = 2/1/1 have the lowest response time (15 s) and recovery time (50 s) for a room temperature operation, proving the key role of carbonic material in shaping the static and dynamic performance of the sensor. These response and recovery times are lower than those of “heated” commercial sensors. The sensing mechanism is explained in terms of the overall response of a p-type semiconductor, where ox-SWCNH percolated between electrodes of the sensor, shunting the heterojunctions made between n-type SnO2 or ZnO and p-type ox-SWCNH. The hard–soft acid–base (HSAB) principle supports this mechanism. The low power consumption of these devices, below 2 mW, and the sensing performances at room temperature may open new avenues towards ethanol sensors for passive samplers of environment monitoring, alcohol test portable instruments and wireless network sensors for Internet of Things applications.
Human Cytomegalovirus-Encoded microRNAs Can Be Found in Saliva Samples from Renal Transplant Recipients
Authors: Waters Shelley , Lee Silvia , Munyard Kylie , Irish Ashley , Price Patricia , Wang Bing H.
Published: December 2020

Human cytomegalovirus (HCMV) infections are common following renal transplantation and may have long-lasting effects. HCMV can be measured directly by viral DNA or indirectly via host immune responses. HCMV-encoded microRNA (miRNA) may alter the pathobiology of HCMV infections and contribute to the progression of HCMV disease. HCMV-encoded miRNAs can be detected in blood but have not been sought in saliva. We investigated saliva samples from 32 renal transplant recipients (RTR) and 12 seropositive healthy controls for whom immunological data was available. Five HCMV-encoded miRNAs (miR-UL112-5p, miR-US5-2-3p, miR-UL36, miR-US25-2-3p and miR-UL22A) were sought using primer probe assays. HCMV miRNA species were detected in saliva from 15 RTR and 3 healthy controls, with miR-US5-2-3p most commonly detected. The presence of HCMV miRNAs associated with increased T-cell responses to HCMV IE-1 in RTR, suggesting a link with frequent reactivations of HCMV.
Survival and Nutritional Status of Male and Female Heart Transplant Patients Based on the Nutritional Risk Index
Authors: Almutawa Deema A. , Almuammar May , Elshafie Mona Mohamed , Aljuraiban Ghadeer S. , Alnafisah Alaa , Abulmeaty Mahmoud M. A.
Published: December 2020

Malnutrition among heart-transplant patients may affect survival. The aim was to investigate the survival and nutrition status among male and female heart transplant patients who underwent transplantation, before and 1 year after surgery based on the nutritional risk index (NRI). The medical records of ninety heart-transplant patients (2009–2014) from the King Faisal Specialist Hospital, Riyadh, were reviewed. The assessment included demographic data, anthropometric measurements, and NRI calculation. Moreover, postoperative data included the length of stay and survival. Paired t-test and survival analysis by Kaplan–Meier (KM) curves were used. A total of 90 patients (males 77.78%) were included. The prevalence of malnutrition in the preoperative phase by NRI was 60% (7.78% as severe; 40% as moderate, and 12.22% mild NRI scores). After 1 year, body mass index (BMI) and NRI increased significantly (p < 0.001). Furthermore, NRI was significantly different between men and women (p < 0.01), while KM survival curves were insignificantly different (p = 0.67). Recipients with postoperative moderate or severe nutritional risk (NRI < 97.5) had significantly shorter survival in the first-year post-transplantation (HR = 0.82; 95% CI, 0.75–0.89; p < 0.001). Our findings indicate that the NRI after 1 year of transplant correlated significantly with mortality. Besides, there was no significant gender difference regarding survival; however, malnutrition and low survival were more prominent among women.
Ketogenic Diet: Impact on Cellular Lipids in Hippocampal Murine Neurons
Authors: Dabke Partha , Brogden Graham , Naim Hassan Y. , Das Anibh M.
Published: December 2020

Background: The mechanism of action of the ketogenic diet (KD), an effective treatment for pharmacotherapy refractory epilepsy, is not fully elucidated. The present study examined the effects of two metabolites accumulating under KD—beta-hydroxybutyrate (ßHB) and decanoic acid (C10) in hippocampal murine (HT22) neurons. Methods: A mouse HT22 hippocampal neuronal cell line was used in the present study. Cellular lipids were analyzed in cell cultures incubated with high (standard) versus low glucose supplemented with ßHB or C10. Cellular cholesterol was analyzed using HPLC, while phospholipids and sphingomyelin (SM) were analyzed using HPTLC. Results: HT22 cells showed higher cholesterol, but lower SM levels in the low glucose group without supplements as compared to the high glucose groups. While cellular cholesterol was reduced in both ßHB- and C10-incubated cells, phospholipids were significantly higher in C10-incubated neurons. Ratios of individual phospholipids to cholesterol were significantly higher in ßHB- and C10-incubated neurons as compared to controls. Conclusion: Changes in the ratios of individual phospholipids to cholesterol in HT22 neurons suggest a possible alteration in the composition of the plasma membrane and organelle membranes, which may provide insight into the working mechanism of KD metabolites ßHB and C10.
Analysis of Sport Supplement Consumption and Body Composition in Spanish Elite Rowers
Authors: Domínguez Raúl , López-Domínguez Rubén , López-Samanes Álvaro , Gené Pol , González-Jurado José Antonio , Sánchez-Oliver Antonio Jesús
Published: December 2020

The aim of this study was to analyze the anthropometric characteristics and sport supplement (SS) consumption patterns of heavyweight and lightweight international rowers. Methods: The 13 heavyweights (11 males) and seven lightweights (five males) of the Spanish National Rowing Team were recruited for the study. Body composition was measured by bio-impedance analysis, and the questionnaire used in this investigation was previously validated to assess SS consumption. According to anthropometrics parameters, it was reported that male heavyweight rowers were heavier (p < 0.001) and taller (p < 0.001), but no statistical differences were reported for % body fat (p = 0.104) or % lean body mass (p = 0.161). All rowers reported consumption of at least one SS. Based on the Australian Institute of Sport’s classification, higher medical supplement consumption was observed when comparing heavyweight rowers to lightweight rowers (2.5 ± 1.1 vs. 1.7 ± 0.5, p = 0.040). There were no differences in the totals of group A (strong scientific evidence for sports scenarios, p = 0.069), group B (emerging scientific support, deserving of further research, p = 0.776), or group C (scientific evidence not supportive of benefit and/or security amongst athletes, p = 0.484). The six most consumed SSs were iron (85%), caffeine (85%), β-alanine (85%), energy bars (85%), vitamin supplements (80%), and isotonic drinks (80%), with no statistical differences between heavyweight and lightweight rowers (p > 0.05). These results suggest that the absence of differences in body composition (expressed as a percentage) do not represent anthropometric disadvantages for heavyweight rowers. In addition, SS consumption was similar between rowers, reporting only higher medical supplement consumption in heavyweight rowers.