bluuebunny/crossref_metadata_2025 huggingface dataset:
High Culturable Bacterial Diversity From a European Desert: The Tabernas Desert
Authors: Molina-Menor Esther , Gimeno-Valero Helena , Pascual Javier , Peretó Juli , Porcar Manuel
Published: January 2021

One of the most diverse ecological niches for microbial bioprospecting is soil, including that of drylands. Drylands are one of the most abundant biomes on Earth, but extreme cases, such as deserts, are considered very rare in Europe. The so-called Tabernas Desert is one of the few examples of a desert area in continental Europe, and although some microbial studies have been performed on this region, a comprehensive strategy to maximize the isolation of environmental bacteria has not been conducted to date. We report here a culturomics approach to study the bacterial diversity of this dryland by using a simple strategy consisting of combining different media, using serial dilutions of the nutrients, and using extended incubation times. With this strategy, we were able to set a large (254 strains) collection of bacteria, the majority of which (93%) were identified through 16S ribosomal RNA (rRNA) gene amplification and sequencing. A significant fraction of the collection consisted of Actinobacteria and Proteobacteria, as well as Firmicutes strains. Among the 254 isolates, 37 different genera were represented, and a high number of possible new taxa were identified (31%), of which, three new Kineococcus species. Moreover, 5 out of the 13 genera represented by one isolate were also possible new species. Specifically, the sequences of 80 isolates held a percentage of identity below the 98.7% threshold considered for potentially new species. These strains belonged to 20 genera. Our results reveal a clear link between medium dilution and isolation of new species, highlight the unexploited bacterial biodiversity of the Tabernas Desert, and evidence the potential of simple strategies to yield surprisingly large numbers of diverse, previously unreported, bacterial strains and species.
Bacterial 3′UTRs: A Useful Resource in Post-transcriptional Regulation
Authors: Menendez-Gil Pilar , Toledo-Arana Alejandro
Published: January 2021

Bacterial messenger RNAs (mRNAs) are composed of 5′ and 3′ untranslated regions (UTRs) that flank the coding sequences (CDSs). In eukaryotes, 3′UTRs play key roles in post-transcriptional regulatory mechanisms. Shortening or deregulation of these regions is associated with diseases such as cancer and metabolic disorders. Comparatively, little is known about the functions of 3′UTRs in bacteria. Over the past few years, 3′UTRs have emerged as important players in the regulation of relevant bacterial processes such as virulence, iron metabolism, and biofilm formation. This MiniReview is an update for the different 3′UTR-mediated mechanisms that regulate gene expression in bacteria. Some of these include 3′UTRs that interact with the 5′UTR of the same transcript to modulate translation, 3′UTRs that are targeted by specific ribonucleases, RNA-binding proteins and small RNAs (sRNAs), and 3′UTRs that act as reservoirs oftrans-acting sRNAs, among others. In addition, recent findings regarding a differential evolution of bacterial 3′UTRs and its impact in the species-specific expression of orthologous genes are also discussed.
IL-37 Confers Anti-Tumor Activity by Regulation of m6A Methylation
Authors: Mu Xiaofeng , Zhao Qi , Chen Wen , Zhao Yuxiang , Yan Qing , Peng Rui , Zhu Jie , Yang Chunrui , Lan Ketao , Gu Xiaosong , Wang Ye
Published: January 2021

N6-methyladenosine (m6A) is a common transcriptomic modification in cancer. Recently, it has been found to be involved in the regulation of non-small cell lung cancer (NSCLC) formation and metastasis. Interleukin 37 (IL-37) plays a crucial protective role in lung cancer. In our previous studies, we found that IL-37 is a potential novel tumor suppressor by inhibiting IL-6 expression to suppress STAT3 activation and decreasing epithelial-to-mesenchymal transition. Moreover, we found that treatment of IL-37 in lung cancer cells induced widespread and dynamic RNA m6A methylation. The effects of RNA m6A methylation of IL-37 treatment require further study. However, the functions of RNA m6A methylation of IL-37 treatment still await elucidation. Using MeRIP-seq and RNA-seq, we uncovered a unique m6A methylation profile in the treatment of IL-37 on the A549 cell line. We also showed the expression of m6A writers METTL3, METTL14, and WTAP and erasers ALKBH5 and FTO in A549 cells and lung cancer tissues after the treatment of IL-37. This study showed that IL-37 could lead to changes in m6A methylation level and related molecule expression level in A546 cells and may downregulate the proliferation by inhibiting Wnt5a/5b pathway in A549 cells. We conclude that IL-37 suppresses tumor growth through regulation of RNA m6A methylation in lung cancer cells.
Blood-Rich Enhancement in Ultrasonography Predicts Worse Prognosis in Patients With Papillary Thyroid Cancer
Authors: Gao Luying , Xi Xuehua , Gao Qiong , Tang Jiajia , Yang Xiao , Zhu Shenling , Zhao Ruina , Lai Xingjian , Zhang Xiaoyan , Zhang Bo , Jiang Yuxin
Published: January 2021

Contrast-enhanced ultrasound (CEUS) can be used to evaluate microcirculation in cancers, which in turn is associated with the biologic features and ultimately patient prognosis. We conducted a retrospective analysis to examine potential association between CEUS parameters and prognosis in patients with papillary thyroid cancer (PTC). The analysis included 306 patients who underwent CEUS prior to thyroidectomy at our center during a period from 2012 to 2019. Subjects with excellent response (ER) were compared to the non-ER group (including indeterminate response, biochemical incomplete response and structural incomplete response). During the median follow-up of 34 months, ER was observed in 195 (63.7%) subjects. The remaining 111 (36.3%) patients developed non-ER events, with distant metastasis in five (1.6%) cases. In a multivariate COX regression, non-ER event was associated with the male sex (OR = 1.83, 95%CI: 1.21–2.76) and blood-rich enhancement in CEUS (OR = 1.69, 95%CI: 1.04–2.75). Based on this finding, we developed a predictive model: high risk for developing non-ER events was defined as having both risk factors; low risk was defined as having none or only one risk. In receiver operating characteristic (ROC) analysis, the area under the curve was 0.59 (95%CI: 0.52–0.66). The sensitivity and specificity were 17.1 and 95.4%, respectively. The positive and negative predictive values were 67.9 and 66.9%, respectively. In conclusion, blood-rich enhancement in CEUS is associated with non-ER events after thyroidectomy in patients with PTC.
Treatment Options of First-Line Tyrosine Kinase Inhibitors and Subsequent Systemic Chemotherapy Agents for Advanced EGFR Mutant Lung Adenocarcinoma Patients: Implications From Taiwan Cancer Registry Cohort
Authors: Liang Sheng-Kai , Keng Li-Ta , Chang Chia-Hao , Wen Yueh-Feng , Lee Meng-Rui , Yang Ching-Yao , Wang Jann-Yuan , Ko Jen-Chung , Shih Jin-Yuan , Yu Chong-Jen
Published: January 2021

Objectives

Large-scale, population-based real-world studies on the treatment outcomes of first-line tyrosine kinase inhibitors (TKIs) and subsequent systemic chemotherapy agents for lung adenocarcinoma (with activating epidermal growth factor receptor [EGFR] mutations) remain limited.

Materials and Methods

From March 2014 to December 2016, patients with advanced lung adenocarcinoma, identified from the Taiwan Cancer Registry were included in this study if they received any of the three TKIs as first-line treatment. The primary outcome was overall survival (OS). The secondary outcome was time-to-treatment discontinuation (TTD).

Results

A total of 4,889 patients (median age: 67 years and two-thirds with distant metastasis) were recruited (1,778 gefitinib, 1,599 erlotinib, and 1,512 afatinib users). A 1:1 propensity score (PS)-matched cohorts of 1,228 afatinib/erlotinib and 1054 afatinib/gefitinib was created. After PS matching, it was found that afatinib was not associated with better OS (afatinib vs. erlotinib, HR: 0.96, 95% CI: 0.86–1.07; afatinib vs. gefitinib, HR: 0.91, 95% CI: 0.81–1.02). In the subgroup analysis, afatinib demonstrated a survival benefit in patients with active smoking (afatinib vs. erlotinib, HR: 0.69, 95% CI: 0.51–0.93; afatinib vs. gefitinib, HR: 0.67, 95% CI: 0.48–0.94) and ECOG > 1 (afatinib vs. erlotinib, HR: 0.79, 95% CI: 0.63–0.99; afatinib vs. gefitinib, HR: 0.78, 95% CI: 0.62–0.98). A total of 41.1% (n = 1992) of first-line TKI users received subsequent chemotherapy. Among the three TKI groups, pemetrexed usage was associated with better OS compared with other chemotherapy agents, with the exception of gemcitabine in the afatinib and gefitinib groups. Pemetrexed and gemcitabine had the longest TTD of 3–4 months.

Conclusions

Among patients with EGFR mutant lung adenocarcinoma, afatinib use may not provide longer OS compared with first-generation TKIs. Afatinib may be preferably considered among patients with active smoking and should not be withheld among those with worse performance status. With 40% of patients receiving subsequent chemotherapy, pemetrexed may be the preferred agent, while gemcitabine can be a reasonable alternative.
Estimating Individualized Absolute Risk for Esophageal Squamous Cell Carcinoma: A Population-Based Study in High-Risk Areas of China
Authors: Shen Yi , Xie Shuanghua , Zhao Lei , Song Guohui , Shao Yi , Hao Changqing , Niu Chen , Ruan Xiaoli , Zang Zhaoping , Nakyeyune Rena , Liu Fen , Wei Wenqiang
Published: January 2021

Background

Esophageal squamous cell carcinoma (ESCC) has a high incidence rate and poor prognosis. In this study, we aimed to develop a predictive model to estimate the individualized 5-year absolute risk for ESCC in Chinese populations living in the high-risk areas of China.

Methods

We developed a risk-predicting model based on the epidemiologic data from a population-based case-control study including 244 newly diagnosed ESCC patients and 1,220 healthy controls. Initially, we included easy-to-obtain risk factors to construct the model using the multivariable logistic regression analysis. The area under the ROC curves (AUC) with cross-validation methods was used to evaluate the performance of the model. Combined with local age- and sex-specific ESCC incidence and mortality rates, the model was then used to estimate the absolute risk of developing ESCC within 5 years.

Results

A relative risk model was established that included eight factors: age, sex, tobacco smoking, alcohol drinking, education, and dietary habits (intake of hot food, intake of pickled/salted food, and intake of fresh fruit). The relative risk model had good discrimination [AUC, 0.785; 95% confidence interval (CI), 0.749–0.821]. The estimated 5-year absolute risk of ESCC for individuals varied widely, from 0.0003% to 19.72% in the studied population, depending on the exposure to risk factors.

Conclusions

Our model based on readily identifiable risk factors showed good discriminative accuracy and strong robustness. And it could be applied to identify individuals with a higher risk of developing ESCC in the Chinese population, who might benefit from further targeted screening to prevent esophageal cancer.
Immune Checkpoint Inhibitors in the Treatment of HCC
Authors: Donisi Clelia , Puzzoni Marco , Ziranu Pina , Lai Eleonora , Mariani Stefano , Saba Giorgio , Impera Valentino , Dubois Marco , Persano Mara , Migliari Marco , Pretta Andrea , Liscia Nicole , Astara Giorgio , Scartozzi Mario
Published: January 2021

Hepatocellular carcinoma (HCC) is the typical inflammation-induced neoplasia. It often prospers where a chronic liver disease persists, thus leading a strong rationale for immune therapy. Several immune-based treatments, including immune checkpoint inhibitors (ICI), cytokines, adoptive cell transfer, and vaccines, have been tested in the treatment of HCC. In this review, we summarize the role of the ICI in HCC patients in various sets of treatment. As for advanced HCC, the anti-Programmed cell Death protein 1 (PD1) antibodies and the anti-Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) antibodies have been examined in patients with enthusiastic results in phase I-II-III studies. Overall, this led the Food and Drug Administration (FDA) to approve pembrolizumab, nivolumab, and nivolumab + ipilimumab in the second-line setting. The anti- Programmed Death-Ligand 1 (PDL-1) antibodies have also been evaluated. Thanks to the results obtained from phase III IMbrave study, atezolizumab + bevacizumab is now the standard of care in the first-line advanced setting of HCC. As for localized HCC, the putative immunological effect of locoregional therapies led to evaluate the combination strategy with ICI. This way, chemoembolization, ablation with radiofrequency, and radioembolization combined with ICI are currently under study. Likewise, the study of adjuvant immunotherapy following surgical resection is underway. In addition, the different ICI has been studied in combination with other ICI as well as with multikinase inhibitors and anti-angiogenesis monoclonal antibody. The evidence available suggests that combining systemic therapies and locoregional treatments with ICI may represent an effective strategy in this context.
Prediction of Response in Head and Neck Tumor: Focus on Main Hot Topics in Research
Authors: Belgioia Liliana , Morbelli Silvia Daniela , Corvò Renzo
Published: January 2021

Radiation therapy is a cornerstone in the treatment of head and neck cancer patients; actually, their management is based on clinical and radiological staging with all patients at the same stage treated in the same way. Recently the increasing knowledge in molecular characterization of head and neck cancer opens the way for a more tailored treatment. Patient outcomes could be improved by a personalized radiotherapy beyond technological and anatomical precision. Several tumor markers are under evaluation to understand their possible prognostic or predictive value. In this paper we discuss those markers specific for evaluate response to radiation therapy in head and neck cancer for a shift toward a biological personalization of radiotherapy.
CircRNAs: Emerging Bladder Cancer Biomarkers and Targets
Authors: Liang Zhaofeng , Guo Wenhao , Fang Shikun , Zhang Yue , Lu Ling , Xu Wenrong , Qian Hui
Published: January 2021

Circular RNAs (circRNAs) are newly discovered intriguing RNAs due to the covalently closed loop structure, high stability, tissue specificity, and functional diversity. In recent years, a large number of circRNAs have been identified through high-throughput sequencing technology and bioinformatics methods, the abnormal expression of circRNAs are closely related to many diseases including bladder cancer (BC). CircRNAs have been proven to have several functions, such as acting as a regulator of parental gene transcription, miRNA sponge and interacting with proteins to regulate its expression. In addition, some circRNAs have been identified to encode proteins. CircRNAs have the characteristics of high abundance, high stability, wide distribution in body fluids, tissue specificity, and developmental stage specificity, which determine that circRNAs has great potential to be utilized as biomarkers for BC. Herein, we briefly summarize the biogenesis, functions and roles, and the current research progress of circRNAs in BC with a focus on the potential application for BC diagnosis, treatment, and prognosis.
TSPAN7 Exerts Anti-Tumor Effects in Bladder Cancer Through the PTEN/PI3K/AKT Pathway
Authors: Yu Xi , Li Shenglan , Pang Mingrui , Du Yang , Xu Tao , Bai Tao , Yang Kang , Hu Juncheng , Zhu Shaoming , Wang Lei , Liu Xiuheng
Published: January 2021

The tetraspanin protein superfamily participate in the dynamic regulation of cellular membrane compartments expressed in a variety of tumor types, which may alter the biological properties of cancer cells such as cell development, activation, growth and motility. The role of tetraspanin 7 (TSPAN7) has never been investigated in bladder cancer (BCa). In this study, we aimed to investigate the biological function of TSPAN7 and its therapeutic potential in human BCa. First, via reverse transcription and quantitative real-time PCR (qRT-PCR), we observed downregulation of TSPAN7 in BCa tissues samples and cell lines and found that this downregulation was associated with a relatively high tumor stage and tumor grade. Low expression of TSPAN7 was significantly correlated with a much poorer prognosis for BCa patients than was high expression. Immunohistochemistry (IHC) showed that low TSPAN7 expression was a high-risk predictor of BCa patient overall survival. Furthermore, the inhibitory effects of TSPAN7 on the proliferation and migration of BCa cell lines were detected by CCK-8, wound-healing, colony formation and transwell assays in vitro. Flow cytometry analysis revealed that TSPAN7 induced BCa cell lines apoptosis and cell cycle arrest. In vivo, tumor growth in nude mice bearing tumor xenografts could be obviously affected by overexpression of TSPAN7. Western blotting showed that overexpression of TSPAN7 activated Bax, cleaved caspase-3 and PTEN but inactivated Bcl-2, p-PI3K, and p-AKT to inhibit BCa cell growth via the PTEN/PI3K/AKT pathway. Taken together, our study will help identify a potential marker for BCa diagnosis and supply a target molecule for BCa treatment.
Efficacy of Indocyanine Green Fluorescence Angiography in Preventing Anastomotic Leakage After McKeown Minimally Invasive Esophagectomy
Authors: Luo Rao-Jun , Zhu Zi-Yi , He Zheng-Fu , Xu Yong , Wang Yun-Zheng , Chen Ping
Published: January 2021

Background

Indocyanine green (ICG) fluorescence angiography (FA) was introduced to provide real-time intraoperative evaluation of the vascular perfusion of the gastric conduit during esophagectomy. However, its efficacy has not yet been proven. The aim of this study was to assess the usefulness of ICG-FA in the reduction of the rates of anastomotic leakage (AL) in McKeown minimally invasive esophagectomy (MIE).

Methods

From June 2017 to December 2019, patients aged between 18 and 80 years with esophageal carcinoma were enrolled in the study and each patient underwent McKeown MIE. Patients were divided into two groups, those with or without ICG-FA. The patient demographics and perioperative outcomes were comparable between the two groups. The primary outcome was the rate of AL.

Results

A total of 192 patients were included: 86 in the ICG-FA group and 106 in the non-ICG-FA group. Overall, 12 patients (6.3%) had AL; the rate of AL was 10.4% in the non-ICG-FA group, which was significantly higher than the 1.2% in the ICG-FA group.

Conclusions

ICG-FA has the potential to reduce the rate of AL in McKeown MIE.
Long-Term Follow-Up and Optimization of Interleukin-1 Inhibitors in the Management of Monogenic Autoinflammatory Diseases: Real-Life Data from the JIR Cohort
Authors: Hentgen Véronique , Koné-Paut Isabelle , Belot Alexandre , Galeotti Caroline , Grateau Gilles , Carbasse Aurelia , Pagnier Anne , Pillet Pascal , Delord Marc , Hofer Michael , Georgin-Lavialle Sophie
Published: January 2021

Objectives: The major role of interleukin (IL)-1 in the pathogenesis of hereditary recurrent fever syndromes favored the employment of targeted therapies modulating IL-1 signaling. However the best use of IL1 inhibitors in terms of dosage is difficult to define at present.

Methods: In order to better understand the use of IL1 inhibitors in a real-life setting, our study assessed the dosage regimens of French patients with one of the four main hereditary recurrent fever syndromes (Familial Mediterranean Fever (FMF), TNF receptor associated periodic syndrome (TRAPS), cryopyrin associated periodic fever (CAPS) and mevalonate kinase deficiency). The patients were retrieved retrospectively from the JIR cohort, an international platform gathering data of patients with pediatric inflammatory diseases.

Results: Forty five patients of the JIR cohort with a hereditary recurrent fever syndrome had received at least once an IL1 inhibitor (anakinra or canakinumab). Of these, 43% received a lower dosage than the one suggested in the product recommendations, regardless of the type of the IL1 inhibitor. Especially patients with FMF and TRAPS seemed to need lower treatment regimens; in our cohort none of the FMF or TRAPS patients received an intensified dose of IL-inhibitor. On-demand treatment with a short half-life IL-1 inhibitor has also been used successfully for some patients with one of these two conditions The standard dose was given to 42% of the patients; whereas an intensified dose of IL-1 inhibitors was given to 15% of the patients (44% of CAPS patients and 17% of mevalonate kinase deficiency patients). In our cohort each individual patient’s need for treatment seemed highly variable, ranging from on demand treatment regimens to intensified dosage maintenance therapies depending on the activity and the severity of the underlying disease.

Conclusion: IL-1 inhibitors are a good treatment option for patients with a hereditary recurrent fever syndrome, but the individual need of the dosage of IL-1 inhibitors to control the disease effectively seems highly variable. Severity, activity but also the type of the underlying disease, belong to the parameters underpinning the treat-to-target strategy implemented in an everyday life practice.
Functional Identification of EjGIF1 in Arabidopsis and Preliminary Analysis of Its Regulatory Mechanisms in the Formation of Triploid Loquat Leaf Heterosis
Authors: Liu Chao , Huang Renwei , Wang Lingli , Liang Guolu
Published: January 2021

Although several results have been obtained in triploid loquat heterosis (i.e., leaf size of triploid loquat) studies in the past years, the underlying mechanisms of the heterosis are still largely unknown, especially the regulation effects of one specific gene on the corresponding morphology heterosis. In this study, we sought to further illustrate the regulatory mechanisms of one specific gene on the leaf size heterosis of triploid loquats. A leaf size development-related gene (EjGIF1) and its promoter were successfully cloned. Ectopic expression ofEjGIF1inArabidopsisshowed that the leaf size of transgenic plantlets was larger than that of WTs, and the transgenic plantlets had more leaves than WTs. Quantitative Reverse Transcription PCR (qRT-PCR) showed that the expression level ofEjGIF1showed an AHP expression pattern in most of the hybrids, and this was consistent with our previous phenotype observations. Structure analysis ofEjGIF1promoter showed that there were significantly more light-responsive elements than other elements. To further ascertain the regulatory mechanisms ofEjGIF1on triploid loquat heterosis, the methylation levels ofEjGIF1promoter in different ploidy loquats were analyzed by using bisulfite sequencing. Surprisingly, the total methylation levels ofEjGIF1promoter in triploid showed a decreasing trend compared with the mid-parent value (MPV), and this was also consistent with the qRT-PCR results ofEjGIF1. Taken together, our results suggested thatEjGIF1played an important role in promoting leaf size development of loquat, and demethylation ofEjGIF1promoter in triploid loquats causedEjGIF1to exhibit over-dominance expression pattern and then further to promote leaf heterosis formation. In conclusion,EjGIF1played an important role in the formation of triploid loquat leaf size heterosis.
Citizens' Assemblies for Referendums and Constitutional Reforms: Is There an “Irish Model” for Deliberative Democracy?
Authors: Courant Dimitri
Published: January 2021

Among democratic innovations, deliberative mini-publics, that is panels of randomly selected citizens tasked to make recommendations about public policies, have been increasingly used. In this regard, Ireland stands out as a truly unique case because, on the one hand, it held four consecutive randomly selected citizens' assemblies, and on the other hand, some of those processes produced major political outcomes through three successful referendums; no other country shows such as record. This led many actors to claim that the “Irish model” was replicable in other countries and that it should lead to political “success.” But is this true? Relying on a qualitative empirical case-study, this article analyses different aspects to answer this question: First, the international context in which the Irish deliberative process took place; second, the differences between the various Irish citizens' assemblies; third, their limitations and issues linked to a contrasted institutionalization; and finally, what “institutional model” emerges from Ireland and whether it can be transferred elsewhere.
Vast Amounts of Encoded Items Nullify but Do Not Reverse the Effect of Sleep on Declarative Memory
Authors: Kolibius Luca D. , Born Jan , Feld Gordon B.
Published: January 2021

Sleep strengthens memories by repeatedly reactivating associated neuron ensembles. Our studies show that although long-term memory for a medium number of word-pairs (160) benefits from sleep, a large number (320) does not. This suggests an upper limit to the amount of information that has access to sleep-dependent declarative memory consolidation, which is possibly linked to the availability of reactivation opportunities. Due to competing processes of global forgetting that are active during sleep, we hypothesized that even larger amounts of information would enhance the proportion of information that is actively forgotten during sleep. In the present study, we aimed to induce such forgetting by challenging the sleeping brain with vast amounts of to be remembered information. For this, 78 participants learned a very large number of 640 word-pairs interspersed with periods of quiet awake rest over the course of an entire day and then either slept or stayed awake during the night. Recall was tested after another night of regular sleep. Results revealed comparable retention rates between the sleep and wake groups. Although this null-effect can be reconciled with the concept of limited capacities available for sleep-dependent consolidation, it contradicts our hypothesis that sleep would increase forgetting compared to the wake group. Additional exploratory analyses relying on equivalence testing and Bayesian statistics reveal that there is evidence against sleep having a detrimental effect on the retention of declarative memory at high information loads. We argue that forgetting occurs in both wake and sleep states through different mechanisms, i.e., through increased interference and through global synaptic downscaling, respectively. Both of these processes might scale similarly with information load.
Loneliness, Emotion Dysregulation, and Internalizing Symptoms During Coronavirus Disease 2019: A Structural Equation Modeling Approach
Authors: Velotti Patrizia , Rogier Guyonne , Beomonte Zobel Sara , Castellano Rosetta , Tambelli Renata
Published: January 2021

Background: Our study aimed to test the hypotheses that an increased level of loneliness experienced during coronavirus disease 2019 (COVID-19) confinement was predictive of internalizing symptoms and that this pathway was mediated by emotion dysregulation levels.

Methods: To reach this aim, we performed an online longitudinal survey recruiting 1,330 participants at Time 1 (at the beginning of the lockdown) and 308 participants at Time 2 (few days before the end of the lockdown). All filled out a set of questionnaires: demographic data, University of California, Los Angeles Loneliness scale, Difficulties in Emotion Regulation Scale−18 items, and Depression Anxiety and Stress Scale−21 items. Hypotheses were tested using structural equation modeling in two steps and controlling for age. First, hypotheses were tested on cross-sectional data. Then, a cross-lagged panel analysis was performed on longitudinal data.

Results: Models obtained a good fit and evidenced the predictive role of loneliness levels on the three outcomes (i.e., depression, anxiety, and stress). Moreover, we found that emotion dysregulation levels partially mediated the longitudinal relationship between loneliness and both depression and stress but not between loneliness and anxiety levels.

Conclusions: This study points out that a central goal of clinical intervention could be the ability to regulate negative emotional states.
Neighborhood Environment and Objectively Measured Sedentary Behavior Among Older Adults: A Cross-Sectional Study
Authors: Chang Shao-Hsi , Rutherford Ru , Hsueh Ming-Chun , Yu Yi-Chien , Park Jong-Hwan , Wang Sendo , Liao Yung
Published: January 2021

Background: We examined the relationships between objectively assessed neighborhood environment and the patterns of sedentary behavior among older adults.

Methods: A total of 126 community-dwelling older adults (aged 65 years or above) were recruited. Data on neighborhood environmental attributes (resident density, street intersection density, sidewalk availability, accessible destinations, and accessible public transportation), accelerometer-assessed total time and patterns of sedentary behavior (number and duration of bouts), and sociodemographic characteristics were collected. Multiple linear regression models were developed.

Results: After adjustment for potential confounders, greater sidewalk availability was negatively related to the number of sedentary bouts (β = −0.185; 95% CI: −0.362, 0.015; p = 0.034) and sedentary bout duration (β = −0.180; 95% CI: −0.354, −0.011; p = 0.037).

Conclusions: This study revealed that a favorable neighborhood environment characterized by sidewalk availability is negatively associated with sedentary behavior patterns in Taiwanese older adults. These findings are critical to inform environmental policy initiatives to prevent sedentary lifestyle in older adults.
Spatial Epidemiology of Salmonellosis in Florida, 2009–2018
Authors: Li Xiaolong , Singh Nitya , Beshearse Elizabeth , Blanton Jason L. , DeMent Jamie , Havelaar Arie H.
Published: January 2021

Non-typhoidal Salmonella enterica infections cause a high disease burden in the United States with an estimated 1.2 million illnesses annually. The state of Florida consistently has a relatively high incidence compared to other states in the United States. Nevertheless, studies regarding the epidemiology of nontyphoidal salmonellosis and its spatial and temporal patterns in Florida were rarely reported. We examined the spatial and temporal patterns of 62,947 salmonellosis cases reported to FL Health Charts between 2009 and 2018. Dominant serotypes circulating in Florida were also explored using whole genome sequencing (WGS) based serotype-prediction for 2,507 Salmonella isolates sequenced by the Florida Department of Health during 2017 and 2018. The representativeness of laboratory-sequenced isolates for reported cases was determined by regression modeling. The annual incidence rate of salmonellosis decreased from 36.0 per 100,000 population in 2009 to 27.8 per 100,000 in 2016, and gradually increased in 2017 and 2018. Increased use of culture-independent testing did not fully explain this increase. The highest incidence rate was observed in children, contributing 40.9% of total reported cases during this period. A seasonal pattern was observed with the incidence peaking in September and October, later than the national average pattern. Over these 10 years, the Northeast and Northwest regions of the state had higher reported incidence rates, while reported rates in the Southeast and South were gradually increasing over time. Serotypes were predicted based on WGS data in the EnteroBase platform. The top-five most prevalent serotypes in Florida during 2017–2018 were Enteritidis, Newport, Javiana, Sandiego and Braenderup. The highest percentage of isolates was from children under 5 years of age (41.4%), and stool (84.7%) was the major source of samples. A zero-inflated negative binomial regression model showed that the reported case number was a strong predictor for the number of lab-sequenced isolates in individual counties, and the geospatial distribution of sequenced isolates was not biased by other factors such as age group. The spatial and temporal patterns identified in this study along with the prevalence of different serotypes will be helpful for the development of efficient prevention and control strategies for salmonellosis in Florida.
Association of Night Sleep Duration and Ideal Cardiovascular Health in Rural China: The Henan Rural Cohort Study
Authors: Wu Xueyan , Liu Xiaotian , Liao Wei , Kang Ning , Sang Shengxiang , Abdulai Tanko , Zhai Zhihan , Wang Chongjian , Wang Xiaoqiong , Li Yuqian
Published: January 2021

Introduction: We aimed to explore the association between night sleep duration and ideal cardiovascular health (ICH) among Chinese rural population.

Methods: In all, 35,094 participants were included from the Henan Rural Cohort study. Information on sleep was collected using the Pittsburgh Sleep Quality Index. The ICH scores were evaluated. The associations between night sleep duration and ICH were examined using both linear regression and logistic regression models.

Results: The mean night sleep duration for all participants was 7.75 ± 1.28 h. Compared with those with night sleep duration of 7 to <9 h by using linear regression model, a significant decrease in ICH scores was observed for participants with shorter [−0.077 (−0.131, −0.024)] and longer [−0.079 (−0.121, −0.036)] night sleep duration. Compared with 7 to <9 h, longer sleep duration [0.919 (0.851, 0.992)] were associated with decreased odds of ideal CVH.

Conclusions: Shorter and longer night sleep duration are negatively associated with ICH among rural population. This suggests that it may be beneficial to include night sleep duration assessment in cardiovascular risk screening.
Real-Time Bibliometrics: Dimensions as a Resource for Analyzing Aspects of COVID-19
Authors: Hook Daniel W , Porter Simon J , Draux Hélène , Herzog Christian T
Published: January 2021

Dimensions was built as a platform to allow stakeholders in the research community, including academic bibliometricians, to more easily create and understand the context of different types of research object through the linkages between these objects. Links between objects are created via persistent identifiers and machine learning techniques, while additional context is introduced via data enhancements such as per-object categorisations and person and institution disambiguation. While these features make analytical use cases accessible for end users, the COVID-19 crisis has highlighted a different set of needs to analyze trends in scholarship as they occur: Real-time bibliometrics. The combination of full-text search, daily data updates, a broad set of scholarly objects including pre-prints and a wider set of data fields for analysis, broadens opportunities for a different style of analysis. A subset of these emerging capabilities is discussed and three basic analyses are presented as illustrations of the potential for real-time bibliometrics.
The Welfare of Beef Cattle in the Scientific Literature From 1990 to 2019: A Text Mining Approach
Authors: Nalon Elena , Contiero Barbara , Gottardo Flaviana , Cozzi Giulio
Published: January 2021

Beef cattle are the third most numerous terrestrial farmed animals worldwide. Factors such as geographical region, animal category, breed, and rearing system pose specific animal welfare challenges that can have an impact on animal and public health. This article uses text mining (TM) and topic analysis (TA) to explore the scientific literature on beef cattle welfare published in English from 1990 to 2019. Our aim was to reveal the main research topics and their evolution over time. Our analysis showed that the three most relevant themes in research since 1990 have to do with calf behaviour and management, efficiency, and environmental sustainability, and the effects of transport and slaughter on meat quality. Topics showing the most marked increase in the number of papers published deal with stakeholders' perceptions and market opportunities for added-value beef products and risk factors for morbidity and mortality, especially in relation to calf health, antimicrobial use, and antimicrobial resistance. The results indicate a particular focus on the welfare of calves, especially in the veal industry. Pain relief during the castration of calves and bulls also featured prominently. Research is also increasingly assessing aspects of beef cattle welfare that are interlinked to meat quality, the social and environmental sustainability of the sector in relation to market opportunities, and public health. The identified topics represent a basic source of information that can be used for further and more detailed analyses (e.g., systematic reviews) focussed on specific research themes or geographical areas.
Resource, Collaborator, or Individual Cow? Applying Q Methodology to Investigate Austrian Farmers' Viewpoints on Motivational Aspects of Improving Animal Welfare
Authors: Maurer Lorenz , Schenkenfelder Josef , Winckler Christoph
Published: January 2021

One keystone to successful welfare improvement endeavors is a respected cooperation between farmer and advisor (e.g., veterinarian), which requires a thorough understanding of what motivates farmer behavior. In this respect, Q methodology offers a promising approach in investigating individual motivational patterns and to discriminate between and describe typologies of farmers. In our study we explored, based on a sample of 34 Austrian dairy farmers, how 39 potentially motivating statements regarding the improvement of dairy cow health and welfare were assessed. We were able to identify and describe four different viewpoints, explaining 47% of total study variance. All four viewpoints have in common that pride in a healthy herd is motivating to work toward improved animal health and welfare to a certain extent, but meeting legal requirements is rather not. Viewpoint 1 acknowledges welfare for economic performance, ease of work and short working hours but does not make allowance for outside interference. Participants loading on Viewpoint 2 also show a focus on economic aspects but, keep close track of the animal welfare debate recognizing its potential to improve the public image of dairy farming. Even though they cautiously criticize an exploitative application of dairy farming, they do not want to be understood as role models. With regards to animal welfare, farmers sharing Viewpoint 3 perceive themselves as superior to and show little reluctance of comparison with mainstream farming. For them, the animal as sentient being itself owns some intrinsic value and it is necessary to strike a balance between economic and other, ethical considerations. Viewpoint 4 perceives cows as equal collaborators who deserve to be treated with respect and appreciation and is willing to accept certain economic losses in order to maintain high standards regarding animal health and welfare. Using Q methodology, we have been able to draw high resolution images of different farmer typologies, enabling advisors to tailor intervention strategies specifically addressing leverage points with a high chance of farmer compliance.
Influence of Storage on Physiological Properties, Chemical Composition, and Bioactive Compounds on Cactus Pear Fruit (Opuntia ficus-indica (L.) Mill.)
Authors: Andreu-Coll Lucía , García-Pastor María Emma , Valero Daniel , Amorós Asunción , Almansa María Soledad , Legua Pilar , Hernández Francisca
Published: January 2021

Cactus pear (Opuntia ficus-indica (L.) Mill.) fruit from ‘Orito’ cultivar were stored at 2 °C and 90% RH for 28 days plus three days at 20 °C (shelf life, SL). This research analysed the changes in fruit quality parameters (weight loss, firmness, color, titratable acidity, and total soluble solids), ethylene production, respiration rate, antioxidant activity and bioactive compounds (total phenols and carotenoids) of cactus pear fruit during cold and shelf life storage. Under cold conditions, CO2 production decreased, and ethylene production increased slightly, while under shelf life conditions CO2 production increased and ethylene production increased more sharply. Firmness increased under cold conditions and did not change during shelf life period. The content of total soluble solids (TSS), titratable acidity (TA), pH, total carotenoids, and lipo-antioxidant activity (L-TAA) remained stable under both conservation conditions. However, hydro-antioxidant activity (H-TAA) increased under both cold and shelf life conditions, and total phenols remained stable during cold storage and increased under shelf life conditions. Besides, weight loss was acceptable under both storage conditions, and color changes were more pronounced under shelf life storage. These results show that the marketability of cactus pear fruit from ‘Orito’ cultivar was acceptable until the end of the storage under cold and shelf life conditions.
Investigating the Thioredoxin and Glutathione Systems’ Response in Lymphoma Cells after Treatment with [Au(d2pype)2]Cl
Authors: Wang Sicong , Lu Yaoying , Woods Kyra , Di Trapani Giovanna , Tonissen Kathryn F.
Published: January 2021

Lymphoma is a blood cancer comprising various subtypes. Although effective therapies are available, some patients fail to respond to treatment and can suffer from side effects. Antioxidant systems, especially the thioredoxin (Trx) and glutathione (GSH) systems, are known to enhance cancer cell survival, with thioredoxin reductase (TrxR) recently reported as a potential anticancer target. Since the GSH system can compensate for some Trx system functions, we investigated its response in three lymphoma cell lines after inhibiting TrxR activity with [Au(d2pype)2]Cl, a known TrxR inhibitor. [Au(d2pype)2]Cl increased intracellular reactive oxygen species (ROS) levels and induced caspase-3 activity leading to cell apoptosis through inhibiting both TrxR and glutathione peroxidase (Gpx) activity. Expression of the tumour suppresser gene TXNIP increased, while GPX1 and GPX4 expression, which are related to poor prognosis of lymphoma patients, decreased. Unlike SUDHL2 and SUDHL4 cells, which exhibited a decreased GSH/GSSG ratio after treatment, in KMH2 cells the ratio remained unchanged, while glutathione reductase and glutaredoxin expression increased. Since KMH2 cells were less sensitive to treatment with [Au(d2pype)2]Cl, the GSH system may play a role in protecting cells from apoptosis after TrxR inhibition. Overall, our study demonstrates that inhibition of TrxR represents a valid therapeutic approach for lymphoma.
The Effect of Superabsorbent Polymers on the Microstructure and Self-Healing Properties of Cementitious-Based Composite Materials
Authors: Kanellopoulou Irene A. , Kartsonakis Ioannis A. , Charitidis Costas A.
Published: January 2021

Cementitious structures have prevailed worldwide and are expected to exhibit further growth in the future. Nevertheless, cement cracking is an issue that needs to be addressed in order to enhance structure durability and sustainability especially when exposed to aggressive environments. The purpose of this work was to examine the impact of the Superabsorbent Polymers (SAPs) incorporation into cementitious composite materials (mortars) with respect to their structure (hybrid structure consisting of organic core—inorganic shell) and evaluate the microstructure and self-healing properties of the obtained mortars. The applied SAPs were tailored to maintain their functionality in the cementitious environment. Control and mortar/SAPs specimens with two different SAPs concentrations (1 and 2% bwoc) were molded and their mechanical properties were determined according to EN 196-1, while their microstructure and self-healing behavior were evaluated via microCT. Compressive strength, a key property for mortars, which often degrades with SAPs incorporation, in this work, practically remained intact for all specimens. This is coherent with the porosity reduction and the narrower range of pore size distribution for the mortar/SAPs specimens as determined via microCT. Moreover, the self-healing behavior of mortar-SAPs specimens was enhanced up to 60% compared to control specimens. Conclusively, the overall SAPs functionality in cementitious-based materials was optimized.
Realisation of a Novel Functionally Redundant Actuation System for a Railway Track-Switch
Authors: Olaby Osama , Dutta Saikat , Harrison Tim , Ward Christopher P. , Dixon Roger
Published: January 2021

This paper focuses on modelling, control, realisation and performance analysis of a full-scale demonstrator for a novel railway track switch. For over a century, railway track switches (or points) have been allowing trains to safely change between routes. As they are safety-critical elements of the rail network, when they fail, the signalling system will prevent trains from using that route. This means poor reliability (or lack of availability) leads to significant delays and costs; hence there is huge interest from researchers and engineers in improving the overall reliability of track switches. This paper presents new results, which represent a meaningful first step toward a revolution in the way track switches are actuated. A “REPOINT-Light” railway track switch demonstrator is introduced which uses a new concept of locking to allow redundant actuation with three actuator bearers operating under closed-loop feedback control. The new concept, its control system and its mechanical viability are tested in experiments at the Great Central Railway in the UK. To support the design of the actuators and the control system, a dynamic simulation model is developed by co-simulation involving Simulink and Simpack. The experimental results presented are used to validate the models and the paper discusses how the models themselves are used as the vehicle for the design of feedback controllers. Virtual testing of the controllers in simulation is a vital step prior to the implementation and deployment of the controllers in the demonstrator switch. The major contribution of this work is demonstrating, for the first time at full scale on a real-world track switch, that it is possible to use one-out-of-three actuator redundancy to provide fault-tolerant operation of railway track switch.
Analysis for the Knowledge Management Application in Maintenance Engineering: Perception from Maintenance Technicians
Authors: Cárcel-Carrasco Javier , Cárcel-Carrasco José-Antonio
Published: January 2021

Knowledge based on personal experience (tacit knowledge) acquired in problem solving actions and in maintenance actions is the fundamental basis for maintenance technicians in companies with great physical assets. Generally, there is no proper policy for managing strategic knowledge and its capture. In this article, through qualitative studies (grounded theory) and surveys conducted with technicians, the aim was to obtain the perception of the maintenance technicians’ part of the companies, in order to establish the characteristics of the relation between the strategic aspects and the engineering aspects of industrial maintenance, regarding knowledge management, as well as the enablers and barriers to its application. The results show how a high level of tacit knowledge is used in this activity, which requires more time for new staff. The values obtained from this survey show that the knowledge recorded by the companies (explicit) is 51.25%, compared to the personal knowledge (tacit) of maintenance technicians regarding reliability and breakdowns. In operational/exploitational actions it is 43.90%, for energy efficiency actions it is 49.61%, and in maintenance actions (preventive, predictive, and corrective) the value is 68.78%. This shows the significant gap between the perception of recorded knowledge (explicit), and the knowledge that maintenance technicians have (tacit knowledge). All this can affect the companies, as part of the strategic knowledge is lost when a maintenance technician leaves the company.