bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind’s Challenges
Authors: El-Gendi Hamada , Saleh Ahmed K. , Badierah Raied , Redwan Elrashdy M. , El-Maradny Yousra A. , El-Fakharany Esmail M.
Published: December 2021

Enzymes have played a crucial role in mankind’s challenges to use different types of biological systems for a diversity of applications. They are proteins that break down and convert complicated compounds to produce simple products. Fungal enzymes are compatible, efficient, and proper products for many uses in medicinal requests, industrial processing, bioremediation purposes, and agricultural applications. Fungal enzymes have appropriate stability to give manufactured products suitable shelf life, affordable cost, and approved demands. Fungal enzymes have been used from ancient times to today in many industries, including baking, brewing, cheese making, antibiotics production, and commodities manufacturing, such as linen and leather. Furthermore, they also are used in other fields such as paper production, detergent, the textile industry, and in drinks and food technology in products manufacturing ranging from tea and coffee to fruit juice and wine. Recently, fungi have been used for the production of more than 50% of the needed enzymes. Fungi can produce different types of enzymes extracellularly, which gives a great chance for producing in large amounts with low cost and easy viability in purified forms using simple purification methods. In the present review, a comprehensive trial has been advanced to elaborate on the different types and structures of fungal enzymes as well as the current status of the uses of fungal enzymes in various applications.
Evaluating Local Attractiveness for Tourism and Recreation—A Case Study of the Communes in Brzeski County, Poland
Authors: Ziernicka-Wojtaszek Agnieszka , Malec Magdalena
Published: December 2021

For many regions and communes, the development of tourism offers opportunities for economic revival, the stimulation of local entrepreneurship and, as a result, increased budget revenues. Before such objectives can be successfully pursued, however, it is necessary to carefully consider to what degree the conditions present in a particular area are conducive to the development of tourism there, including its tourism resources and the existing state of development. This study analyzes the attractiveness of the seven communes making up Brzeski County (Lesser Poland Voivodeship, Poland) for tourism and recreation. The potential of the communes in this largely rural county was characterized using Gołembski’s index, based on multivariate comparative analysis, assessing the natural and man-made features and the degree of development of each of the communes. This analysis was further complemented using Wejchert’s impression curve, assessing visual attractiveness along selected routes in two of the communes. Of the seven communes analyzed, the Commune of Brzesko was found to have the most extensively developed services, hotel and food-service infrastructure, to be home to many historic buildings and to have a relatively high municipal budget. The Commune of Iwkowa, in turn, which scored lowest in terms of Gołembski’s index (mainly due to its underdeveloped infrastructure, few historic buildings and the fact that it is crossed by a major transit route), was nevertheless found to score significantly higher than Brzesko in terms of Wejchert’s impression curve. These findings confirm that the beauty of the landscape is not always well correlated with tourism development potential. We conclude that such a combination of methods should to be applied to reliably and comprehensively evaluate the relative attractiveness of different parts of a given region for tourism and recreation, for instance so as to inform planning decisions and the allocation of funding.
The Impact of China Carbon Emission Trading System on Land Use Transition: A Macroscopic Economic Perspective
Authors: Tang Yingkai , Yang Yunfan , Xu He
Published: December 2021

The carbon emission trading system (CETS) is a milestone policy in the history of China’s emission trading system, which is of great significance to China’s realization of “carbon peak and carbon neutralization”. As an important component of sustainable development, LUT should be related to the CETS. However, in the literature on the CETS, little material deals with its impact on land use transition (LUT). This paper will enrich this literature. Based on 30 provincial regions in China from 2011 to 2017, using the DID and entropy methods, this study investigated the impact of CETS on the trend of LUT from three perspectives: economic effects, environmental effects and Porter effects. The conclusions are that (1) the implementation of the CETS hindered economic development, but optimized energy-use efficiency; (2) the implementation of the CETS reduced the emissions of CO2 and SO2; (3) the implementation of the CETS did not produce a Porter effect; and (4) the influence of the CETS had the characteristics of a spatial cluster. These findings offer some guidance for improving CETS policies and formulating similar environmental regulation policies.
Pain Reduction during Rapid Palatal Expansion Due to LED Photobiomodulation Irradiation: A Randomized Clinical Trial
Authors: Caccianiga Gianluigi , Caccianiga Paolo , Baldoni Marco , Lo Giudice Antonino , Perillo Letizia , Moretti Nicolò , Ceraulo Saverio
Published: December 2021

Objective: The purpose of this research is to assess the analgesic efficiency of Photobiomodulation (PBM) in pain reduction in young patients during rapid maxillary expansion therapy. Materials and Methods: Thirty patients were included and allocated to an experimental group [Rapid Palatal Expansion (RPE) and PBM] and a control group (RPE only) at random. Inclusion criteria were skeletal age from CVS1 to CVS3, examined on the cephalometric lateral teleradiography, with cervical vertebra staging and completed eruption of upper first molar. Exclusion criteria were previous orthodontic treatment, bone disease, disability, or skeletal age from CVS4. Patients referred the pain they felt using a Numerical scale rate (NRS), ranging from 0 to 10, with specific intervals of time: 6 h, 12 h, 24 h, and from days 2 to 7. The Wilcoxon-Mann-Whitney test was used to evaluate differences in NRS reported values between the two groups. Results: The final sample included 30 patients, 14 males and 16 females, with a mean age of 7.8 ± 1.2 years. The pain that was felt at each time interval and the maximum score of pain were significantly lower in the experimental group (p < 0.05) and decreased faster in the experimental group, with a score test near to 0 after 2/3 days. Conclusions: PBM is efficient in reducing the intensity and the time of pain felt by young patients that undergo rapid maxillary expansion.
Targeting Oxidative Phosphorylation-Proteasome Activity in Extracellular Detached Cells Promotes Anoikis and Inhibits Metastasis
Authors: Adeshakin Funmilayo O. , Adeshakin Adeleye O. , Liu Zhao , Cheng Jian , Zhang Pengchao , Yan Dehong , Zhang Guizhong , Wan Xiaochun
Published: December 2021

Metastasis arises owing to tumor cells’ capacity to evade pro-apoptotic signals. Anoikis—the apoptosis of detached cells (from the extracellular matrix (ECM)) is often circumvented by metastatic cells as a result of biochemical and molecular transformations. These facilitate cells’ ability to survive, invade and reattach to secondary sites. Here, we identified deregulated glucose metabolism, oxidative phosphorylation, and proteasome in anchorage-independent cells compared to adherent cells. Metformin an anti-diabetic drug that reduces blood glucose (also known to inhibit mitochondrial Complex I), and proteasome inhibitors were employed to target these changes. Metformin or proteasome inhibitors alone increased misfolded protein accumulation, sensitized tumor cells to anoikis, and impaired pulmonary metastasis in the B16F10 melanoma model. Mechanistically, metformin reduced cellular ATP production, activated AMPK to foster pro-apoptotic unfolded protein response (UPR) through enhanced expression of CHOP in ECM detached cells. Furthermore, AMPK inhibition reduced misfolded protein accumulation, thus highlight relevance of AMPK activation in facilitating metformin-induced stress and UPR cell death. Our findings provide insights into the molecular biology of anoikis resistance and identified metformin and proteasome inhibitors as potential therapeutic options for tumor metastasis.
Resistance of Aluminide Coatings on Austenitic Stainless Steel in a Nitriding Atmosphere
Authors: Wierzbowska Karolina , Kochmańska Agnieszka Elżbieta , Kochmański Paweł
Published: December 2021

A new slurry cementation method was used to produce silicide-aluminide protective coatings on austenitic stainless steel 1.4541. The slurry cementation processes were carried out at temperatures of 800 and 1000 °C for 2 h with and without an additional oxidation process at a temperature of 1000 °C for 5 min. The microstructure and thickness of the coatings were studied by scanning electron microscopy (SEM). The intention was to produce coatings that would increase the heat resistance of the steel in a nitriding atmosphere. For this reason, the produced coatings were subjected to gas nitriding at a temperature of 550–570 °C in an atmosphere containing from 40 to 60% of ammonia. The nitriding was carried out using four time steps: 16, 51, 124, and 200 h, and microstructural observations using SEM were performed after each step. Analysis of the chemical composition of the aluminide coatings and reference sample was performed using wavelength (WDS) and energy (EDS) dispersive X-ray microanalysis, and phase analysis was carried out using X-ray diffraction (XRD). The resistance of the aluminide coatings in the nitriding atmosphere was found to depend strongly on the phase composition of the coating. The greatest increase in resistance to gas corrosion under nitriding atmosphere conditions was achieved using a manufacturing temperature of 1000 °C.
Surface and Subsurface Quality of Titanium Grade 23 Machined by Electro Discharge Machining
Authors: Karmiris-Obratański Panagiotis , Papazoglou Emmanouil L. , Leszczyńska-Madej Beata , Zagórski Krzysztof , Markopoulos Angelos P.
Published: December 2021

Electrical Discharge Machining (EDM) is a non-traditional cutting technology that is extensively utilized in contemporary industry, particularly for machining difficult-to-cut materials. EDM may be used to create complicated forms and geometries with great dimensional precision. Titanium alloys are widely used in high-end applications owing to their unique intrinsic characteristics. Nonetheless, they have low machinability. The current paper includes an experimental examination of EDM’s Ti-6Al-4V ELI (Extra Low Interstitials through controlled interstitial element levels) process utilizing a graphite electrode. The pulse-on current (IP) and pulse-on time (Ton) were used as control parameters, and machining performance was measured in terms of Material Removal Rate (MRR), Tool Material Removal Rate (TMRR), and Tool Wear Ratio (TWR). The Surface Roughness (SR) was estimated based on the mean roughness (SRa) and maximum peak to valley height (SRz), while, the EDMed surfaces were also examined using optical and SEM microscopy and cross-sections to determine the Average White Layer Thickness (AWLT). Finally, for the indices above, Analysis of Variance (ANOVA) was conducted, whilst semi-empirical correlations for the MRR and TMRR were given using the Response Surface Method (RSM). The results show that the pulse-on time is the most significant parameter of the machining process that may increase the MRR up to 354%. Pulse-on current and pulse-on time are shown to have an impact on the surface integrity of the finished product. Furthermore, statistics, SEM, and EDX images on material removal efficiency and tool wear rate are offered to support the core causes of surface and sub-surface damage. The average microhardness of the White Layer (WL) is 1786 HV.
Recent Developments in Non-Conventional Welding of Materials
Authors: Leal Rui M. , Galvão Ivan
Published: December 2021

Welding is one of the technological fields with the greatest impact in many industries, such as automotive, aerospace, energy production, electronics, the health sector, etc. [...]
A Comprehensive Review of Characterization Methods for Metallurgical Coke Structures
Authors: Zheng Heng , Xu Runsheng , Zhang Jianliang , Daghagheleh Oday , Schenk Johannes , Li Chuanhui , Wang Wei
Published: December 2021

The structure of coke affects its reactivity and strength, which directly influences its performance in the blast furnace. This review divides coke structures into chemical structure, physical structure, and optical texture according to their relevant characteristics. The focuses of this review are the current characterization methods and research status of the coke structures. The chemical structures (element composition and functional group) can be characterized by elemental analysis, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (Raman), X-ray photoelectron spectroscopy (XPS), and nuclear magnetic resonance imaging technology (13C NMR). The physical structures (pore structure and micro-crystallite structure) can be characterized by image method, X-ray CT imaging technique, mercury intrusion method, nitrogen gas adsorption method, X-ray diffraction method (XRD), and high-resolution transmission electron microscopy (HRTEM). The optical textures are usually divided and counted by a polarizing microscope. In the end, this review provides an idea of the construction of a coke molecular structural model, based on the above characterization. With the coke model, the evolution principles of the coke can be calculated and simulated. Hence, the coke performance can be predicted and optimized.
Performance Evaluation of Plastic Concrete Modified with E-Waste Plastic as a Partial Replacement of Coarse Aggregate
Authors: Ahmad Farhan , Jamal Arshad , Mazher Khwaja Mateen , Umer Waleed , Iqbal Mudassir
Published: December 2021

Plastic electronic waste (E-waste) is constantly growing around the world owing to the rapid increase in industrialization, urbanization, and population. The current annual production rate of E-waste is 3–4% in the world and is expected to increase to 55 million tons per year by 2025. To reduce the detrimental impact on the environment and save natural resources, one of the best solutions is to incorporate waste plastic in the construction industry to produce green concrete. This study examines the use of manufactured plastic coarse aggregate (PCA) obtained from E-waste as a partial replacement of natural coarse aggregate (NCA) in concrete. Six types of concrete mix with 10%, 20%, 30%, 40%, and 50% substitution of NCA (by volume) with PCA are prepared and tested. This study investigates the effect of manufactured PCA on the fresh and hardened characteristics of concrete. The properties of recycled plastic aggregate concrete (RPAC) studied include workability, fresh density, dry density, compressive strength (CS), splitting tensile strength (STS), flexural strength (FS), sorptivity coefficient, abrasion resistance, ultrasonic pulse velocity (UPV), and alternate wetting and drying (W–D). The results indicate that the CS, STS, and FS of RPAC declined in the range of 9.9–52.7%, 7.8–47.5%, and 11–39.4%, respectively, for substitution ratios of 10–50%. However, the results also indicate that the incorporation of PCA (10–50%) improved the workability and durability characteristics of concrete. A significant decrement in the sorptivity coefficient, abrasion loss, and UPV value was observed with an increasing amount of PCA. Furthermore, RPAC containing different percentages of PCA revealed better results against alternate W–D cycles with respect to ordinary concrete.
Investigation of Effect of Preliminary Annealing on Superplasticity of Ultrafine-Grained Conductor Aluminum Alloys Al-0.5%Mg-Sc
Authors: Gryaznov Mikhail , Shotin Sergey , Nokhrin Aleksey , Chuvil’deev Vladimir , Likhnitskii Constantine , Kopylov Vladimir , Chegurov Mikhail , Tabachkova Nataliya , Shadrina Iana , Smirnova Elena , Pirozhnikova Olga
Published: December 2021

Effect of preliminary precipitation of Al3Sc particles on the characteristics of superplastic conductor Al-0.5%Mg-X%Sc (X = 0.2, 0.3, 0.4, 0.5 wt.%) alloys with ultrafine-grained (UFG) microstructure has been studied. The precipitation of the Al3Sc particles took place during long-time annealing of the alloys at 300 °C. The preliminary annealing was shown to affect the superplasticity characteristics of the UFG Al-0.5%Mg-X%Sc alloys (the elongation to failure, yield stress, dynamic grain growth rate) weakly but to promote more intensive pore formation and to reduce the volume fraction of the recrystallized microstructure in the deformed and non-deformed parts of the aluminum alloy specimens. The dynamic grain growth was shown to go in the deformed specimen material nonuniformly–the maximum volume fraction of the recrystallized microstructure was observed in the regions of the localization of plastic deformation.
Tribotechnical Properties of Sintered Antifriction Aluminum-Based Composite under Dry Friction against Steel
Authors: Rusin Nikolay M. , Skorentsev Alexander L. , Krinitcyn Maksim G. , Dmitriev Andrey I.
Published: December 2021

The disadvantage of antifriction Al–Sn alloys with high tin content is their low bearing capacity. To improve this property, the aluminum matrix of the alloys was alloyed with zinc. The powder of Al–10Zn alloy was blended with the powder of pure tin in the proportion of 40/60 (wt.%). The resulting mixture of the powders was compacted in briquettes and sintered in a vacuum furnace. The sintered briquettes were subjected to subsequent pressing in the closed press mold at an elevated temperature. After this processing, the yield strength of the sintered (Al–10Zn)–40Sn composite was 1.6 times higher than that of the two-phase Al–40Sn one. The tribological tests of the composites were carried out according to the pin-on-disk scheme without lubrication at pressures of 1–5 MPa. It was established that the (Al–10Zn)–40Sn composite has higher wear resistance compared with the Al–40Sn one. However, this advantage becomes insignificant with an increase in the pressure. It was found that the main wear mechanism of the investigated composites under the dry friction process is a delamination of their highly deformed matrix grains.
Investigation of Thermal Stability of Microstructure and Mechanical Properties of Bimetallic Fine-Grained Wires from Al–0.25%Zr–(Sc,Hf) Alloys
Authors: Nokhrin Aleksey , Shadrina Iana , Chuvil’deev Vladimir , Kopylov Vladimir , Berendeev Nikolay , Murashov Artem , Bobrov Aleksandr , Tabachkova Nataliya , Smirnova Elena , Faddeev Mikhail
Published: December 2021

Thermal stability of composite bimetallic wires from five novel microalloyed aluminum alloys with different contents of alloying elements (Zr, Sc, and Hf) is investigated. The alloy workpieces were obtained by induction-casting in a vacuum, preliminary severe plastic deformation, and annealing providing the formation of a uniform microstructure and the nucleation of stabilizing intermetallide Al3(Zr,Sc,Hf) nanoparticles. The wires of 0.26 mm in diameter were obtained by simultaneous deformation of the Al alloy with Cu shell. The bimetallic wires demonstrated high strength and improved thermal stability. After annealing at 450–500 °C, a uniform fine-grained microstructure formed in the wire (the mean grain sizes in the annealed Al wires are 3–5 μm). An increased hardness and strength due to nucleation of the Al3(Sc,Hf) particles was observed. A diffusion of Cu from the shell into the surface layers of the Al wire was observed when heating up to 400–450 °C. The Cu diffusion depth into the annealed Al wire surfaces reached 30–40 μm. The maximum elongation to failure of the wires (20–30%) was achieved after annealing at 350 °C. The maximum values of microhardness (Hv = 500–520 MPa) and of ultimate strength (σb = 195–235 MPa) after annealing at 500 °C were observed for the wires made from the Al alloys alloyed with 0.05–0.1% Sc.
Effect of Conditioning on PU Foam Matrix Materials Properties
Authors: Lapčík Lubomír , Vašina Martin , Lapčíková Barbora , Murtaja Yousef
Published: December 2021

This article deals with the characterization of the thermal-induced aging of soft polyurethane (PU) foams. There are studied thermal and mechanical properties by means of thermal analysis, tensile, compression and dynamic mechanical vibration testing. It was found in this study, that the increasing relative humidity of the surrounding atmosphere leads to the initiation of the degradation processes. This is reflected in the observed decreased mechanical stiffness. It is attributed to the plasticization of the PU foams wall material. It is in agreement with the observed increase of the permanent deformation accompanied simultaneously with the decrease of Young’s modulus of elasticity. The latter phenomenon is studied by the novel non-destructive forced oscillations vibration-damping testing, which is confirmed by observed lower mechanical stiffness thus indicating the loss of the elasticity induced by samples conditioning. In parallel, observed decreasing of the matrix hardness is confirming the loss of elastic mechanical performance as well. The effect of conditioning leads to the significant loss of the PU foam’s thermal stability.
Texture Evolution during Recrystallization and Grain Growth in Non-Oriented Electrical Steel Produced by Compact Strip Production Process
Authors: Cong Jun-Qiang , Guo Fei-Hu , Qiao Jia-Long , Qiu Sheng-Tao , Wang Hai-Jun
Published: December 2021

Evolution of texture and α*-fiber texture formation mechanism of Fe-0.65%Si non-oriented electrical steel produced by Compact Strip Production (CSP) process during all the thermo-mechanical processing steps were investigated using electron backscatter diffraction (EBSD) and X-ray diffraction (XRD) techniques. Columnar crystal structure of cast slab is fine and well-developed. Textures of the hot-rolled band are quite different in the thickness direction. During annealing of cold-rolled sheet, γ-fiber texture grains would nucleate and grow preferentially, and α*-fiber texture grains mainly nucleate and grow in the shear zone of α-fiber texture of cold-rolled sheet. During the recrystallization process, γ-fiber texture gradually concentrated to {111}<112>, and γ and α*-fiber texture increased significantly. {111}<112> texture priority nucleation at the initial stage of recrystallization. Due to the advantages of nucleation position and quantity, the content of α*-fiber texture is greater than {111}<112> texture in the mid-recrystallization. During grain growth process, {111}<112> oriented grains would grow selectively by virtue of higher mobility, sizes and quantity advantages than that of {411}<148 > and {100}<120>, resulting in the gradual increase of γ-fiber texture and the decline of α *-fiber texture.
Structure and Magnetic Properties of Intermetallic Rare-Earth-Transition-Metal Compounds: A Review
Authors: Bessais Lotfi
Published: December 2021

This review discusses the properties of candidate compounds for semi-hard and hard magnetic applications. Their general formula is R1−sT5+2s with R = rare earth, T = transition metal and 0≤s≤0.5 and among them, the focus will be on the ThMn12- and Th2Zn17-type structures. Not only will the influence of the structure on the magnetic properties be shown, but also the influence of various R and T elements on the intrinsic magnetic properties will be discussed (R = Y, Pr, Nd, Sm, Gd, … and T = Fe, Co, Si, Al, Ga, Mo, Zr, Cr, Ti, V, …). The influence of the microstructure on the extrinsic magnetic properties of these R–T based intermetallic nanomaterials, prepared by high energy ball milling followed by short annealing, will be also be shown. In addition, the electronic structure studied by DFT will be presented and compared to the results of experimental magnetic measurements as well as the hyperfine parameter determined by Mössbauer spectrometry.
Hydration–Strength–Workability–Durability of Binary, Ternary, and Quaternary Composite Pastes
Authors: Han Yi , Oh Seokhoon , Wang Xiao-Yong , Lin Run-Sheng
Published: December 2021

At present, reducing carbon emissions is an urgent problem that needs to be solved in the cement industry. This study used three mineral admixtures materials: limestone powder (0–10%), metakaolin (0–15%), and fly ash (0–30%). Binary, ternary, and quaternary pastes were prepared, and the specimens’ workability, compressive strength, ultrasonic pulse speed, surface resistivity, and the heat of hydration were studied; X-ray diffraction and attenuated total reflection Fourier transform infrared tests were conducted. In addition, the influence of supplementary cementitious materials on the compressive strength and durability of the blended paste and the sustainable development of the quaternary-blended paste was analyzed. The experimental results are summarized as follows: (1) metakaolin can reduce the workability of cement paste; (2) the addition of alternative materials can promote cement hydration and help improve long-term compressive strength; (3) surface resistivity tests show that adding alternative materials can increase the value of surface resistivity; (4) the quaternary-blended paste can greatly reduce the accumulated heat of hydration; (5) increasing the amount of supplementary cementitious materials can effectively reduce carbon emissions compared with pure cement paste. In summary, the quaternary-blended paste has great advantages in terms of durability and sustainability and has good development prospects.
Effect of Topical PTH 1-34 Functionalized to Biogran® in the Process of Alveolar Repair in Rats Submitted to Orchiectomy
Authors: Frigério Paula Buzo , Gomes-Ferreira Pedro Henrique Silva , de Souza Batista Fábio Roberto , Moura Juliana , Rangel Garcia Júnior Idelmo , Botticelli Daniele , Lisboa-Filho Paulo Noronha , Okamoto Roberta
Published: December 2021

(1) Background: There are many therapies for osteoporosis control and bone maintenance; anabolic drugs such as teriparatide and bone grafts help in the repair process and stimulate bone formation. Thus, the aim of the present study was to evaluate the behavior of repaired bone in the presence of PTH (teriparatide) associated with Biogran® (biomaterial) through a sonochemical procedure after extraction in rats. (2) Methods: The insertion of Biogran® with PTH in the alveolus was performed 30 days after incisor extraction. Euthanasia occurred after 60 days. (3) Results: The use of local treatment of PTH loaded with Biogran® in healthy rats promoted good results for micro-CT, with an increase in percentage and bone volume, number and trabecular separation and less total porosity. Greater immunostaining for Wnt, β-Catenin and osteocalcin proteins and lower expression for Thrombospondin-Related Adhesive Protein (TRAP), which shows an increase in the number of osteoblasts and inhibition of osteoclast action. However, the treated orchiectomized groups did not obtain such expressive results. (4) Conclusion: The use of Biogran® with PTH improved alveolar repair in rats. However, new researches with more efficient doses must be studied to collaborate effectively with the formation of a quality bone after the orchiectomy.
Optimization of Machining Parameters for Milling Zirconia Ceramics by Polycrystalline Diamond Tool
Authors: Yan Xuefeng , Dong Shuliang , Li Xianzhun , Zhao Zhonglin , Dong Shuling , An Libao
Published: December 2021

Zirconia ceramics are widely used in many fields because of their excellent physical and mechanical properties. However, there are some challenges to machine zirconia ceramics with high processing efficiency. In order to optimize parameters for milling zirconia ceramics by polycrystalline diamond tool, finite element method was used to simulate machining process based on Johnson-Cook constitutive model. The effects of spindle speed, feed rate, radial and axial cutting depth on cutting force, tool flank wear and material removal rate were investigated. The results of the simulation experiment were analyzed and optimized by the response surface method. The optimal parameter combination was obtained when the spindle speed, feed rate, radial and axial cutting depth were 8000 r/min, 90.65 mm/min, 0.10 mm and 1.37 mm, respectively. Under these conditions, the cutting force was 234.81 N, the tool flank wear was 33.40 μm when the milling length was 60 mm and the material removal rate was 44.65 mm3/min.
Antifouling Activity of Halogenated Compounds Derived from the Red Alga Sphaerococcus coronopifolius: Potential for the Development of Environmentally Friendly Solutions
Authors: Quémener Maxence , Kikionis Stefanos , Fauchon Marilyne , Toueix Yannick , Aulanier Fanny , Makris Antonios M. , Roussis Vassilios , Ioannou Efstathia , Hellio Claire
Published: December 2021

Nowadays, biofouling is responsible for enormous economic losses in the maritime sector, and its treatment with conventional antifouling paints is causing significant problems to the environment. Biomimetism and green chemistry approaches are very promising research strategies for the discovery of new antifouling compounds. This study focused on the red alga Sphaerococcus coronopifolius, which is known as a producer of bioactive secondary metabolites. Fifteen compounds, including bromosphaerol (1), were tested against key marine biofoulers (five marine bacteria and three microalgae) and two enzymes associated with the adhesion process in macroalgae and invertebrates. Each metabolite presented antifouling activity against at least one organism/enzyme. This investigation also revealed that two compounds, sphaerococcinol A (4) and 14R-hydroxy-13,14-dihydro-sphaerococcinol A (5), were the most potent compounds without toxicity towards oyster larvae used as non-target organisms. These compounds are of high potential as they are active towards key biofoulers and could be produced by a cultivable alga, a fact that is important from the green chemistry point of view.
Cognitive Behavioral Therapy for Migraine Headache: A Systematic Review and Meta-Analysis
Authors: Bae Ji-yong , Sung Hyun-Kyung , Kwon Na-Yoen , Go Ho-Yeon , Kim Tae-jeong , Shin Seon-Mi , Lee Sangkwan
Published: December 2021

Background and Objectives: Migraine headaches are chronic neurological diseases that reduce the quality of life by causing severe headaches and autonomic nervous system dysfunction, such as facial flushing, nasal stuffiness, and sweating. Their major treatment methods include medication and cognitive behavioral therapy (CBT). CBT has been used for pain treatment and various psychogenic neurological diseases by reducing pain, disability, and emotional disorders caused by symptoms of mental illness and improving the understanding of mental health. This study aimed to evaluate the effectiveness and safety of CBT in treating migraines. Materials and Methods: Seven electronic databases were searched from the date of inception to December 2020. Randomized controlled studies (RCTs) using CBT as an intervention for migraine were included. The primary outcome of this study was to determine the frequency of migraines and the intensity of migraines on Visual Analog Scale (VAS), the frequency of drug use, Migraine Disability Assessment (MIDAS), and Headache Impact Test (HIT-6) index. The two authors independently conducted the data extraction and quality assessment of the included RCTs, and conducted meta-analysis with RevMan V.5.4. Results: Among the 373 studies, 11 RCTs were included in this systematic review. Seven out of the 11 RCTs were conducted in the USA, and four were conducted in the UK, Germany, Iran, and Italy, respectively. Headache frequency and MIDAS scores were statistically significant reduced. In the subgroup analysis, headache strength was significantly reduced. Two of the included studies reported adverse effects, including worsening of migraine intensity and frequency, respiratory symptoms, and vivid memory of a traumatic event. Conclusions: CBT for migraine effectively reduced headache frequency and MIDAS score in meta-analysis and headache intensity subgroup analysis, with few adverse events. Additional RCTs with CBT for migraine headaches are needed for a more accurate analysis.
Optimization of a MOF Blended with Modified Polyimide Membrane for High-Performance Gas Separation
Authors: Zhang Yushu , Jia Hongge , Wang Qingji , Ma Wenqiang , Yang Guoxing , Xu Shuangping , Li Shaobin , Su Guiming , Qu Yanqing , Zhang Mingyu , Jiang Pengfei
Published: December 2021

The preparation, characterization and gas separation properties of mixed matrix membranes (MMMs) were obtained from polyimide capped with ionic liquid and blended with metal-organic frameworks (MOFs). The synthesized MOF was amine functionalized to produce UiO-66-NH2, and its amino group has a higher affinity for CO2. Mixed matrix membranes exhibited good membrane forming ability, heat resistance and mechanical properties. The polyimide membrane exclusively capped by ionic liquid exhibited good permselectivity of 74.1 for CO2/CH4, which was 6.2 times that of the pure polyimide membrane. It is worth noting that MMM blended with UiO-66-NH2 demonstrated the highest ideal selectivity for CO2/CH4 (95.1) with a CO2 permeability of 7.61 Barrer, which is close to the 2008 Robeson upper bound. The addition of UiO-66-NH2 and ionic liquid enhanced the permselectivity of MMMs, which may be one of the promising technologies for high performance CO2/CH4 gas separation.
Lipid Specific Membrane Interaction of Aptamers and Cytotoxicity
Authors: Ashrafuzzaman Md. , AlMansour Hanouf A. M. , AlOtaibi Maha A. S. , Khan Zahid , Shaik Gouse M.
Published: December 2021

We aim to discover diagnostic tools to detect phosphatidylserine (PS) externalization on apoptotic cell surface using PS binding aptamers, AAAGAC and TAAAGA, and hence to understand chemotherapy drug efficacy when inducing apoptosis into cancer cells. The entropic fragment-based approach designed aptamers have been investigated to inspect three aspects: lipid specificity in aptamers’ membrane binding and bilayer physical properties-induced regulation of binding mechanisms, the apoptosis-induced cancer cell surface binding of aptamers, and the aptamer-induced cytotoxicity. The liposome binding assays show preferred membrane binding of aptamers due to presence of PS in predominantly phosphatidylcholine-contained liposomes. Two membrane stiffness reducing amphiphiles triton X-100 and capsaicin were found to enhance membrane’s aptamer adsorption suggesting that bilayer physical properties influence membrane’s adsorption of drugs. Microscopic images of fluorescence-tagged aptamer treated LoVo cells show strong fluorescence intensity only if apoptosis is induced. Aptamers find enhanced PS molecules to bind with on the surface of apoptotic over nonapoptotic cells. In cytotoxicity experiments, TAAAGA (over poor PS binding aptamer CAGAAAAAAAC) was found cytotoxic towards RBL cells due to perhaps binding with nonapoptotic externalized PS randomly and thus slowly breaching plasma membrane integrity. In these three experimental investigations, we found aptamers to act on membranes at comparable concentrations and specifically with PS binding manner. Earlier, we reported the origins of actions through molecular mechanism studies—aptamers interact with lipids using mainly charge-based interactions. Lipids and aptamers hold distinguishable charge properties, and hence, lipid–aptamer association follows distinguishable energetics due to electrostatic and van der Waals interactions. We discover that our PS binding aptamers, due to lipid-specific interactions, appear as diagnostic tools capable of detecting drug-induced apoptosis in cancer cells.
Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications
Authors: Kiradzhiyska Denitsa , Milcheva Nikolina , Mancheva Rositsa , Batsalova Tsvetelina , Dzhambazov Balik , Zahariev Nikolay
Published: December 2021

The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al2O3 coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al2O3 pads indicated by increased percent cell survival following in vitro culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application.
Explicit Analysis of Sheet Metal Forming Processes Using Solid-Shell Elements
Authors: Li Qiao-Min , Yi Zhao-Wei , Liu Yu-Qi , Tang Xue-Feng , Jiang Wei , Li Hong-Jun
Published: December 2021

To simulate sheet metal forming processes precisely, an in-house dynamic explicit code was developed to apply a new solid-shell element to sheet metal forming analyses, with a corotational coordinate system utilized to simplify the nonlinearity and to integrate the element with anisotropic constitutive laws. The enhancing parameter of the solid-shell element, implemented to circumvent the volumetric and thickness locking phenomena, was condensed into an explicit form. To avoid the rank deficiency, a modified physical stabilization involving the B-bar method and reconstruction of transverse shear components was adopted. For computational efficiency of the solid-shell element in numerical applications, an adaptive mesh subdivision scheme was developed, with element geometry and contact condition taken as subdivision criteria. To accurately capture the anisotropic behavior of sheet metals, material models with three different anisotropic yield functions were incorporated. Several numerical examples were carried out to validate the accuracy of the proposed element and the efficiency of the adaptive mesh subdivision.
Formability Prediction of Laser-Welded Stainless Steel AISI 304 and AISI 430
Authors: Evin Emil , Tomáš Miroslav
Published: December 2021

The effect of laser welding on the mechanical properties and the prediction of formability for austenitic stainless steel AISI 304 and ferritic steel AISI 430 when welded by a YLS-5000 fiber laser, were studied in the paper. The microstructure of the welded joint was analyzed using light microscopy. The mechanical properties were determined by static tensile testing. The forming limit diagrams were produced from notched samples at R5, R17, and R25 mm. The hardness values of the welded joint and the base material were determined using the Vickers method. Samples made of AISI 430 showed that the formability suffered due to laser welding. Longitudinal coarse ferrite grains were observed in the microstructure of the AISI 430 weld metal. The coarse-grained structure of the welded joint and the continuous interface along the centerline caused the failure of the AISI 430 laser-welded samples at significantly lower actual stress and strain values than were required to break the base material. No significant changes in the formability were observed in the AISI 304 samples after laser welding. The growth of dendrites was observed in the microstructure of the AISI 304 welded joint in a direction towards the centerline of the welded joint. A comparison of the experimentally determined FLD0 values and the values calculated from predictive equations showed that a better agreement was achieved for uniform elongation than for the strain hardening exponent. The manufacturability and economic efficiency of selected parts of an exhaust system by hydromechanical drawing were evaluated on the basis of the process capability index Cpk.
Electrodeposition of Indium from an Ionic Liquid Investigated by In Situ Electrochemical XPS
Authors: Liu Zhen , Cheng Jun , Höfft Oliver , Endres Frank
Published: December 2021

The electrochemical behavior and electrodeposition of indium in an electrolyte composed of 0.1 mol/L InCl3 in 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)amide ([Py1,4]TFSI) on a gold electrode were investigated. The cyclic voltammogram revealed several reduction and oxidation peaks, indicating a complex electrochemical behavior. In the cathodic regime, with the formation of an In-Au alloy, the reduction of In(III) to In(I) and of In(I) to In(0) takes place. In situ electrochemical X-ray photoelectron spectroscopy (XPS) was employed to investigate the reduction process by monitoring the oxidation states of the components during the cathodic polarization of 0.1 mol/L InCl3/[Py1,4]TFSI on a gold working electrode under ultra-high vacuum (UHV) conditions. The core electron binding energies of the IL components (C 1s, O 1s, F 1s, N 1s, and S 2p) shift almost linearly to more negative values as a function of the applied cell voltage. At −2.0 V versus Pt-quasi reference, In(I) was identified as the intermediate species during the reduction process. In the anodic regime, a strong increase in the pressure in the XPS chamber was recorded at a cell voltage of more than −0.5 V versus Pt quasi reference, which indicated, in addition to the oxidation reactions of In species, that the oxidation of Cl− occurs. Ex situ XPS and XRD results revealed the formation of metallic In and of an In-Au alloy.
Complex Material and Surface Analysis of Anterolateral Distal Tibial Plate of 1.4441 Steel
Authors: Hlinka Josef , Dostalova Kamila , Dedkova Katerina Peterek , Madeja Roman , Frydrysek Karel , Koutecky Jan , Sova Pavel , Douglas Timothy E. L.
Published: December 2021

Nickel-based austenitic stainless steels are still common for manufacture of implants intended for acute hard tissue reinforcement or stabilization, but the risk of negative reactions due to soluble nickel-rich corrosion products must be considered seriously. Corrosion processes may even be accelerated by the evolution of microstructure caused by excessive heat during machining, etc. Therefore, this study also deals with the investigation of microstructure and microhardness changes near the threaded holes of the anterolateral distal tibial plate containing approx. 14wt.% Ni by composition. There were only insignificant changes of microhardness, grain size, or microstructure orientation found close to the area of machining. In addition, wettability measurements of surface energy demonstrated only minor differences for bulk material and areas close to machining. The cyclic potentiodynamic polarization tests were performed in isotonic physiological solution. The first cycle was used for the determination of corrosion characteristics of the implant after chemical passivation, the second cycle was used to simulate real material behavior under the condition of previous surface damage by excessive pitting corrosion occurring during previous polarization. It was found that the damaged and spontaneously repassived surface showed a three-time higher standard corrosion rate than the “as received” chemically passivated surface. One may conclude that previous surface damage may decrease the lifetime of the implant significantly and increase the amount of nickel-based corrosion products distributed into surrounding tissues.
Regional Brain Analysis of Modified Amino Acids and Dipeptides during the Sleep/Wake Cycle
Authors: Vallianatou Theodosia , Bèchet Nicholas B. , Correia Mario S. P. , Lundgaard Iben , Globisch Daniel
Published: December 2021

Sleep is a state in which important restorative and anabolic processes occur. Understanding changes of these metabolic processes during the circadian rhythm in the brain is crucial to elucidate neurophysiological mechanisms important for sleep function. Investigation of amino acid modifications and dipeptides has recently emerged as a valuable approach in the metabolic profiling of the central nervous system. Nonetheless, very little is known about the effects of sleep on the brain levels of amino acid analogues. In the present study, we examined brain regional sleep-induced alterations selective for modified amino acids and dipeptides using Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) based metabolomics. Our approach enabled the detection and identification of numerous amino acid-containing metabolites in the cortex, the hippocampus, the midbrain, and the cerebellum. In particular, analogues of the aromatic amino acids phenylalanine, tyrosine and tryptophan were significantly altered during sleep in the investigated brain regions. Cortical levels of medium and long chain N-acyl glycines were higher during sleep. Regional specific changes were also detected, especially related to tyrosine analogues in the hippocampus and the cerebellum. Our findings demonstrate a strong correlation between circadian rhythms and amino acid metabolism specific for different brain regions that provide previously unknown insights in brain metabolism.
Methotrexate Disposition, Anti-Folate Activity, and Metabolomic Profiling to Identify Molecular Markers of Disease Activity and Drug Response in the Collagen-Induced Arthritis Mouse Model
Authors: Salamoun Yezan M. , Polireddy Kishore , Cho Yu Kyoung , Medcalf Matthew R. , Funk Ryan S.
Published: December 2021

Methotrexate (MTX) is widely used in the treatment of autoimmune arthritis but is limited by its unpredictable and variable response profile. Currently, no biomarkers exist to predict or monitor early therapeutic responses to MTX. Using a collagen-induced arthritis (CIA) mouse model, this study aimed to identify biochemical pathways and biomarkers associated with MTX efficacy in autoimmune arthritis. Following arthritis disease induction, DBA/1J mice were treated with subcutaneous MTX (20 mg/kg/week) and disease activity was assessed based on disease activity scores (DAS) and paw volume (PV) measurements. Red blood cell (RBC) and plasma samples were collected at the end of the study and were assessed for folate and MTX content. Plasma samples were analyzed by semitargeted global metabolomic profiling and analyzed by univariate and multivariate analysis. Treatment with MTX was associated with significant reductions in disease activity based on both DAS (p = 0.0006) and PV (p = 0.0006). MTX therapy resulted in significant reductions in 5-methyltetrahydrofolate (5mTHF) levels in plasma (p = 0.02) and RBCs (p = 0.001). Reductions in both RBC and plasma 5mTHF were associated with lower DAS (p = 0.0007, p = 0.01, respectively) and PV (p = 0.001, p = 0.005, respectively). Increases in RBC MTX were associated with lower DAS (p = 0.003) but not PV (p = 0.23). Metabolomic analysis identified N-methylisoleucine (NMI) and quinolone as metabolites significantly altered in disease mice, which were corrected towards healthy control levels in mice treated with MTX. Reductions in plasma NMI were associated with lower DAS (p = 0.0002) and PV (p = 9.5 × 10−6). Increases in plasma quinolone were associated with lower DAS (p = 0.02) and PV (p = 0.01). Receiver-operating characteristic curve analysis identified plasma NMI (AUC = 1.00, p = 2.4 × 10−8), RBC 5mTHF (AUC = 0.99, p = 2.4 × 10−5), and plasma quinolone (AUC = 0.89, p = 0.01) as top discriminating metabolites of MTX treatment. Our data support a relationship between MTX efficacy and its effect on circulating folates and identified 5mTHF, NMI, and quinolone as potential therapeutic biomarkers of disease activity and MTX response in the CIA mouse model of autoimmune arthritis.