bluuebunny/crossref_metadata_2025 huggingface dataset:
Lactobacillus fermentum 016 Alleviates Mice Colitis by Modulating Oxidative Stress, Gut Microbiota, and Microbial Metabolism
Authors: Pan Huachun , Yang Shumin , Kulyar Md. F. , Ma Hongwei , Li Kewei , Zhang Lihong , Mo Quan , Li Jiakui
Published: January 2025

Ulcerative colitis (UC) is a chronic and progressive inflammatory gastrointestinal disease closely associated with gut microbiota dysbiosis and metabolic homeostasis disruption. Although targeted microbial therapies are an emerging intervention strategy for inflammatory bowel disease (IBD), the mechanisms by which specific probiotics, such as Lactobacillus fermentum 016 (LF), alleviate UC remain unclear. The current study evaluated the effects of LF supplementation on gut health in a basal model using C57BL/6 mice. Subsequently, the preventive effects and mechanisms of LF supplementation on DSS-induced UC were systematically investigated. According to our findings, LF supplementation revealed immunoregulatory capabilities with significantly altered gut the composition of microbiota and metabolic activities, particularly enhancing tryptophan metabolism. In the UC model, LF supplementation effectively mitigated weight loss, increased the disease activity index (DAI), and alleviated diarrhea, rectal bleeding, and colon shortening. Moreover, it reduced colonic pathological damage and histological injury scores. LF intervention improved antioxidant markers and intestinal mucosal barrier function with the activation of the Nrf2–Keap1 signaling pathway and regulation of systemic inflammatory markers, i.e., IL-1β, IL-6, TNF-α, IFN-γ, IL-4, and IL-10. Importantly, LF supplementation reversed metabolic disturbances by significantly increasing the abundance of beneficial genera (e.g., g_Dubosiella, g_Faecalibaculum, g_Odoribacter, g_Candidatus_saccharimonas, g_Roseburia, and g_Eubacterium_xylanophilum_group) and elevating tryptophan metabolites (e.g., melatonin, kynurenic acid, 3-indoleacetic acid, 5-methoxytryptophan, and 5-hydroxyindoleacetic acid). In conclusion, Lactobacillus fermentum 016 exhibits potential for regulating gut microbiota homeostasis, enhancing tryptophan metabolism, and alleviating UC, providing critical insights for developing probiotic-based precision therapeutic strategies for IBD.
Current Evidence on the Impact of Diet, Food, and Supplement Intake on Breast Cancer Health Outcomes in Patients Undergoing Endocrine Therapy
Authors: Žuža Praštalo Milena , Pokimica Biljana , Arsić Aleksandra , Ilich Jasminka Z. , Vučić Vesna
Published: January 2025

Background/Objectives: The most common type of breast cancer (BRC) in women is estrogen/progesterone receptor positive. First-line treatment includes endocrine therapy, either with aromatase inhibitors or tamoxifen to reduce estrogen levels. Among the side effects produced by this treatment, aromatase inhibitor-induced arthralgia is the most common, affecting the patients’ overall health and quality of life (QoL). The objectives here were to evaluate interventions examining the impact of modified diets, supplements, and/or some food components on health outcomes in BRC patients undergoing endocrine therapy. Methods: The literature search was performed in PubMed, Scopus, and Web of Science from June 2024, as well as manually, through the end of November 2024. The search was limited to studies of women diagnosed with estrogen/progesterone-receptor-positive BRC with selected articles reporting interventions with diet, food, or supplement intake and examining the relevant health outcomes. Studies not focusing on BRC patients undergoing endocrine therapy or not including specific health outcomes were excluded. Results: The search uncovered 1028 studies; after the removal of duplicates, abstracts, and irrelevant studies, 53 were closely examined, with 26 evaluated and presented here. The outcomes were changes in bone and body composition, cardiovascular disease risks, inflammation, and QoL. Conclusions: The examined evidence suggests that adherence to dietary patterns such as the Mediterranean or a low-fat diet, and a higher intake of fruits and vegetables were beneficial for various outcomes. Additionally, supplementation with some foods/components (dried plum, red clover) contributed to improving/maintaining bone and body composition, especially in overweight/obese patients. Supplementation with vitamin D or omega-3 improved lipid and angiogenic parameters and QoL. Although these results are promising, the effects of each supplement/food cannot be summarized due to the diverse nature of study designs, patients, and supplement dosages. Further studies are needed to explore the effects of specific nutritional interventions (including the newest, like fasting-mimicking diets and whole-grain cereal diets) on various health outcomes in BRC survivors during endocrine therapy, and to derive universal recommendations.
The Impact of Glomerular Disease on Dyslipidemia in Pediatric Patients Treated with Dialysis
Authors: Zitnik Edward , Streja Elani , Laster Marciana
Published: January 2025

Background/Objectives: Children on dialysis have a 10-fold increase in cardiovascular disease (CVD)-related mortality when compared to the general population. The development of CVD in dialysis patients is attributed to Chronic Kidney Disease–Mineral Bone Disorder (CKD-MBD) and dyslipidemia. While the prevalence of dyslipidemia in adult dialysis patients has been described, there are limited data on prevalence, severity, and risk factors for pediatric dyslipidemia. Methods: Data from 1730 pediatric patients ≤ 21 years receiving maintenance hemodialysis or peritoneal dialysis with at least one lipid panel measurement were obtained from USRDS between 2001 and 2016. Disease etiology was classified as being glomerular (n = 1029) or non-glomerular (n = 701). Comparisons were made across etiologies using both linear and logistic regression models to determine the relationship between disease etiology and lipid levels. Results: The cohort had a mean age of 15.2 years and were 54.5% female. Adjusting for age, sex, race/ethnicity, modality, time with End Stage Kidney Disease (ESKD), and body mass index (BMI) and using non-glomerular etiology as the reference, glomerular disease [mean (95% CI)] was associated with +19% (+14.7%, +23.8%) higher total cholesterol level (183 mg/dL vs. 162 mg/dL), +21% (+14.8%, +26.6%) higher low density lipoprotein cholesterol level (108 mg/dL vs. 87 mg/dL), and +22.3% (+15.5%, +29.5%) higher triglyceride level (169 mg/dL vs. 147 mg/dL). Glomerular disease [OR (95% CI)] was associated with 3.0-fold [2.4, 3.9] higher odds of having an abnormal total cholesterol level, 3.8-fold [2.8, 5.0] higher odds of having an abnormal LDL-C level, and 1.9-fold [1.5, 2.4] higher odds of having an abnormal triglyceride level when compared to non-glomerular disease. Conclusions: Pediatric dialysis patients have a high prevalence of dyslipidemia, particularly from elevated triglyceride levels. Specifically, patients with glomerular disease have an even higher risk of dyslipidemia from elevated non-HDL cholesterol and triglyceride levels than patients with non-glomerular disease. The long-term impact of this unfavorable lipid profile requires further investigation.
Risk-Weight Calculation of Candidate Risk Factors for Incidental Osteoporotic Fracture in Patients with Rheumatic Diseases: A Potentially Accurate Approach
Authors: Yoshii Ichiro , Sawada Naoya , Chijiwa Tatsumi
Published: January 2025

Background/Objectives: To assess the risk of osteoporotic fractures in patients with rheumatic diseases (RDs), we introduced a new approach for predicting incident osteoporotic fractures (OF), employing a risk-weight calculation for each candidate risk factor. Methods: RD outpatients were picked up, and their histories, including OFs, were studied. A Cox regression analysis that evaluated candidate risk factors was conducted with a multivariate model. The variants were selected as candidate risk factors that showed statistical significance using a univariate model. Using the risk ratio or the β-value and p-value, different approaches to acquire a total risk weight (TRW) for each patient were determined to compare the sensitivity and specificity among the approach methods. The cut-off index (COI) was determined using receiver operating characteristic analysis. Sensitivity and specificity for incident OFs were determined using the Kaplan–Meier survival analysis. Results: In a total of 1228 patients, incidental OF occurred in 179 (14.58%) who were included. Factors with significantly higher risk ratios were a history of vertebral and non-vertebral fractures (p < 0.001), cognitive impairment (p < 0.001), anti-osteoporosis drug intervention (p < 0.001), and rehabilitation (p < 0.001). The excellent approach to acquire the best sensitivity and specificity was to calculate the β-value multiplied by the logarithm of the p-value based on 0.05, including non-significant factors (sensitivity: 31.2%, specificity: 94.9%, and area under the curve (AUC): 0.774) compared to 29.4%, 91.6%, and 0.723, respectively, with a counted significant risk factors approach. Conclusions: This novel approach, which includes non-significant factors, can achieve a more accurate sensitivity and specificity to accidental OF in patients with RDs.
Intrathymic Hemagioma: A Challenging Case Report with Special Focus on the Importance of Its Multidisciplinary Approach
Authors: Velimirovici Milan , Popoiu Anca Voichita , Cerbu Simona , Popoiu Calin Marius , Dorobantu Florica Ramona , Caplar Borislav Dusan , Melnic Eugen , Cimpean Anca Maria , Tomescu Larisa Cristina , Stanciulescu Maria Corina
Published: January 2025

Mediastinal hemangiomas, particularly those of thymic origin, are rare phenomena. Due to its rarity, this pathologic condition is not characterized as related to the angiogenic profile of hemangioma endothelial cells. The diagnosis is challenging clinically and radiologically, and biopsies may not yield a definitive answer. Surgical resection offers the material for histologic diagnosis, relieves symptoms, and has a favorable long-term prognosis for such benign tumors. Sometimes, such benign tumors may have aggressive behavior and repeated recurrences but the causes responsible for this unpredictable evolution are not actually known. A case of intrathymic hemangioma diagnosed in a 16-year-old girl is presented here. We focused equally on a multidisciplinary approach to this challenging diagnosis but also on the characterization of the hemangioma endothelial cells profile not previously performed for such type of vascular anomalies. To define an antibodies panel for the evaluation of intrathymic hemangiomas may help in the full characterization of this rare vascular lesion, and subsequently focus on the new therapeutic targets which may be applied for cases with aggressive behavior.
Psychosocial Well-Being in Families of Autistic Children and Effect of Service Dogs: An Exploratory Study
Authors: Dollion Nicolas , Babka Jennifer R. , Pollock Kelly E. , Stilley Julie A. , Grandgeorge Marine , Carlisle Gretchen
Published: January 2025

Service dogs (SDs) have been shown to provide multi-faceted benefits for autistic children, with some benefits also extending to family members. The main objectives of this study were to explore the relationships between psychosocial well-being, family functioning, parent anxiety, and parent–child relationships in families of autistic children, and to investigate the effects of integration of an SD. A cross-sectional approach was used to collect data from three groups of parents of autistic children: (1) families approved for an SD but waitlisted, (2) families in the training process but not yet paired with an SD, and (3) families with a child matched with an SD. Parents completed an online questionnaire including validated tools. Children’s autism-related symptoms correlated with different aspects of family experience and quality of life, and parents’ anxiety scores were related to children’s social anxiety and difficulties associated with parenting an autistic child. Family functioning was associated with children’s autistic characteristics, parents’ anxiety, and the quality of the parent–child relationship. In families of children paired with an SD, children’s autistic symptoms were related to parental attachment with the SD. These findings underscore the need for further research on the assessment and implications of autism-related symptoms for children acquiring an SD.
Urinary Dopamine Levels Can Predict the Avidity of Post-Therapy [131I]MIBG Scintigraphy in Unresectable or Metastatic Pheochromocytomas and Paragangliomas: A Preliminary Clinical Study
Authors: Takenaka Junki , Watanabe Shiro , Abe Takashige , Takeuchi Satoshi , Hirata Kenji , Kimura Rina , Ishii Hiroshi , Wakabayashi Naoto , Majigsuren Mungunkhuyag , Kudo Kohsuke
Published: January 2025

Background/Objectives: Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors that produce catecholamines. Unresectable or metastatic PPGLs are treated with [131I]metaiodobenzylguanidine (MIBG), but MIBG avidity is often heterogeneous. Identifying predictive factors for non-avid lesions on scintigraphy is clinically important. The primary objective of this study was to investigate the relationship between MIBG avidity and catecholamine secretion patterns in patients with unresectable or metastatic PPGLs. Methods: This retrospective study included 27 patients treated with [131I]MIBG for unresectable/metastatic PPGLs between 2001 and 2024. Patients received a single intravenous dose of [131I]MIBG (5.5–7.4 GBq), with post-therapy scintigraphy performed 3–7 days later. Non-avid lesions were assessed by imaging and confirmed using CT, MRI, and FDG-PET. Clinical factors, including age, sex, prior treatments, metastasis sites, and urine catecholamines, were evaluated using univariate logistic analysis. Predictive factors were assessed via receiver operating characteristic curves. Results: Non-avid lesions were found in nine patients (33.3%). These patients were younger (median age 38 vs. 62.5 years) and had higher urine dopamine levels (median 1510 vs. 779 μg/day) than those without non-avid lesions. Younger age (odds ratio: 0.892, p < 0.01) and higher urinary dopamine levels (odds ratio: 1.003, p < 0.01) were significantly associated with non-avid lesions. All patients > 45 years with urinary dopamine < 1190 μg/day had no non-avid lesions, whereas patients < 45 years with urinary dopamine > 1190 μg/day had non-avid lesions. Conclusions: Age and urinary dopamine levels may predict non-avid lesions in unresectable/metastatic PPGLs, aiding treatment decisions for [131I]MIBG therapy. This article is a revised and expanded version of a paper entitled “Urine dopamine level and age can predict non-avid lesion on scintigraphy after I-131 MIBG treatment for unresectable/metastatic PPGL”, which was presented at SNMMI 2024, Toronto, from 8 June to 11 June 2024.
Environmentally Friendly Synthesis of New Mono- and Bis-Pyrazole Derivatives; In Vitro Antimicrobial, Antifungal, and Antioxidant Activity; and In Silico Studies: DFT, ADMETox, and Molecular Docking
Authors: Merzouki Oussama , Arrousse Nadia , Ech-chihbi Elhachmia , Alanazi Ashwag S. , Mabrouk El Houssine , Hefnawy Mohamed , El Moussaoui Abdelfattah , Touijer Hanane , El Barnossi Azeddin , Taleb Mustapha
Published: January 2025

Background/Objectives: Antimicrobial resistance and oxidative stress are major global health challenges, necessitating the development of novel therapeutic agents. Pyrazole derivatives, known for their diverse pharmacological properties, hold promise in addressing these issues. This study aimed to synthesize new mono- and bis-pyrazole derivatives using an eco-friendly, catalyst-free approach and evaluate their antioxidant, antibacterial, and antifungal activities, supported by in silico ADMET profiling, molecular docking, and Density Functional Theory (DFT) analysis. Methods: The compounds were synthesized via a green condensation reaction and characterized using NMR and mass spectrometry, which was verified by DFT analysis. Biological activities were assessed through DPPH and FRAP antioxidant assays, as well as disk diffusion and MIC methods, against bacterial strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and fungal strains (Candida albicans and Aspergillus niger). Computational ADMET profiling evaluated pharmacokinetics and toxicity, while molecular docking assessed interactions with target proteins, including catalase, topoisomerase IV, and CYP51. Results: Theoretical calculations using DFT were in agreement with the experimental results; regarding biological activities, O4 demonstrated the most significant antioxidant activity, with 80.14% DPPH radical scavenging and an IC50 value of 40.91 µg/mL. It exhibited potent antimicrobial activity, surpassing Streptomycin with a 30 mm inhibition zone against Pseudomonas aeruginosa and showing strong efficacy against Staphylococcus aureus and Candida albicans. Computational studies confirmed favorable pharmacokinetic properties, no AMES toxicity, and strong binding affinities. DFT analysis revealed O4’s stability and reactivity, further validating its potential as a therapeutic candidate. Conclusions: This study identified and characterized novel pyrazole derivatives with promising biological and pharmacological properties. O4 emerged as the most potent compound, demonstrating strong antioxidant and antimicrobial activities alongside favorable computational profiles. These findings highlight the potential of the synthetized compounds for therapeutic development and underscore the value of integrating green synthesis with computational techniques in drug discovery.
Flavonoids as Potential Modulators of Pancreatic Lipase Catalytic Activity
Authors: Rocha Sílvia , Proença Carina , Araújo Alberto N. , Freitas Marisa , Rufino Ismael , Aniceto Natália , Silva Artur M. S. , Carvalho Félix , Guedes Rita C. , Fernandes Eduarda
Published: January 2025

Background/Objectives: Obesity has reached pandemic proportions, with predictions suggesting that, by 2030, over 1.5 billion people will be affected. Pancreatic lipase (PL), the enzyme primarily responsible for the absorption of dietary lipids, presents a potential target for obesity management. However, while porcine pancreatic lipase (PPL) is commonly used as the enzyme source for screening potential inhibitors, its effect on human pancreatic lipase (HPL) is rarely reported. This work aimed to screen the inhibitory effects of a library of flavonoids with different functional groups on the activity of PL from the human pancreas (triacylglycerol acyl hydrolase, EC 3.1.1.3) and compare it to the effects of the porcine pancreas (type II, EC 3.1.1.3), establishing, whenever possible, a structure–activity relationship. Methods: The inhibitory effects of a library of 48 flavonoids with different hydroxy, glycosyl, rutinosyl, galloyl, and extended alkyl groups were evaluated against PPL and HPL. The kinetic parameters and inhibitory mechanisms of the most active flavonoids were determined, and in silico docking studies of the more potent flavonoids were also performed, using the active site of HPL. Results/Conclusions: Variations in enzyme catalytic activity were observed depending on the source of the enzyme. The inhibitory effect was particularly influenced by the presence of extended alkyl groups at the C-3 of the C-ring and the C2=C3 double bond of the C-ring and the presence of a pyrogallol group at the C-2′, C-3′ and C-4′ of the B-ring. Docking results showed a strong correlation between docking scores and observed inhibitory activities, highlighting the critical role of specific substituents on the flavonoid backbone in enhancing detailed interaction dynamics with key amino acids. Compounds 28, 29, and 30, with alkyl groups, showed the highest docking scores, interacting with residues HIS151, PHE215, ARG256, and HIS263. Further analysis also revealed that specific substituents improved pocket occupancy and formed additional interactions with residues TYR114, PRO180, ILE209, and PHE215, which are crucial for inhibition. These binding characteristics closely mimic those observed with orlistat, reinforcing their mechanistic similarities in inhibiting HPL and validating their inhibitory activities.
Development, Characterization, and Evaluation of Chi-Tn mAb-Functionalized DOTAP-PLGA Hybrid Nanoparticles Loaded with Docetaxel for Lung Cancer Therapy
Authors: Castro Analía , Pittini Álvaro , Berois Nora , Faccio Ricardo , Miranda Pablo , Mombrú Álvaro W. , Osinaga Eduardo , Pardo Helena
Published: January 2025

Background/Objectives: The focus of this study was to prepare and characterize docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded with DCX and functionalized them with Chi-Tn mAb through a biotin–avidin approach. The physicochemical characterization involved dynamic light scattering, transmission electron microscopy, Raman spectroscopy, and atomic force microscopy. The in vitro and in vivo evaluations encompassed uptake studies, cell viability tests, and the assessment of tumor growth control in a lung cancer model. Results: The nanoparticles featured a hydrophobic PLGA core with 99.9% DCX encapsulation efficiency, surrounded by a DOTAP lipid shell ensuring colloidal stability with a high positive surface charge. The incorporation of PEGylated lipids on their surface helps evade the immune system and facilitate Chi-Tn mAb attachment. The resulting nanoparticles exhibit a spherical shape with monodisperse particle sizes averaging 250 nm, and demonstrate sustained drug release. In vitro uptake studies and viability assays conducted in A549 cancer cells show that the Chi-Tn mAb enhances nanoparticle internalization and significantly reduces cell viability. In vivo studies demonstrate a notable reduction in tumor volume and an increased survival rate in the A549 tumor xenograft mice model when DCX was encapsulated in nanoparticles and targeted with Chi-Tn mAb in comparison to the free drug. Conclusions: Therefore, Chi-Tn-functionalized LPHNps hold promise as carriers for actively targeting DCX to Tn-expressing carcinomas.
Array-Patterned Micro-Structures in Spectacle Lenses Designed for Myopia Control via Image Blur
Authors: Jiang Huilv , Zhao Zengwei , Yuan Quan , Li Yiqian , Ma Ke , Fu Yaoyao , Chen Jiaojie , Jiang Jun , Li Yiyu
Published: January 2025

Using micro-structure components in spectacle lenses has enabled myopia progression control in children and teenagers. However, the optical design of these spectacle lenses has never been discussed, leading to a lack of correct understanding of the underlying optical treatment principles. In this work, array-patterned hexagonal lenslets with two powers of opposite signs were proposed to construct a lenslet array-integrated (LARI) spectacle lens developed for an ongoing, randomized, controlled clinical trial and to support the optical approach to myopia control leveraging retinal image blur. We found that the phase modulation induced by the micro-structures of the lenslet array contributes to the increase in RMS wavefront aberrations, leading to image blur, further inspiring the novel array-patterned micro-structure design with high-order phase elements (HOPEs). The optical performance of both LARI and HOPE spectacle lenses was investigated by simulation and experiment.
Optimization of Erbium-Doped Fiber to Improve Temperature Stability and Efficiency of ASE Sources
Authors: Guo Jia , Zhang Hao , Lin Wenbin , Xu Wei
Published: January 2025

The ASE (Amplified Spontaneous Emission) light source, based on erbium-doped fiber (EDF), is a broadband light source with advantages such as high power, excellent temperature stability, and low coherent light generation. It is widely used in the field of fiber optic sensing. However, traditional ASE sources suffer from temperature sensitivity and low efficiency, which can compromise the accuracy and stability of the output light’s average wavelength. This study focuses on optimizing the erbium-doped fiber (EDF) to improve the temperature stability and efficiency of the ASE light source. Through simulations, we found that the appropriate doping concentration and length of the EDF are key factors in enhancing the stability and efficiency of the ASE source. Inorganic metal chloride vapor-phase doping combined with an improved chemical vapor deposition process was used to fabricate the erbium-doped fiber, ensuring low background loss, minimal OH− absorption, and uniform distribution of the erbium ions in the core of the fiber. The optimized EDFs were integrated into the ASE source, achieving a power conversion efficiency of 53.6% and a temperature stability of 0.118 ppm/°C within the temperature range of −50 °C to 70 °C. This study offers a practical approach for improving the performance of ASE light sources and advancing the development of high-precision fiber optic sensing technologies.
Synergistic Role of Streptomyces Composite Inoculants in Mitigating Wheat Drought Stress Under Field Conditions
Authors: Shan Hao , Wen Hongwei , Zhang Jinhui , Wang Yuzhi , Lu Lahu , Liu Yutao , Yang Bin , Ji Wei
Published: January 2025

Wheat (Triticum aestivum L.) is a globally important staple crop; however, its growth and yield are severely limited by drought stress. This study evaluated the effects of a combined microbial inoculant, Streptomyces pactum Act12 and Streptomyces rochei D74, on wheat photosynthesis, physiological traits, and yield under drought conditions. Key physiological and yield parameters were measured during the jointing, heading, and grain-filling stages. Drought stress significantly reduced chlorophyll content, maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm), and antioxidant enzyme activities, while increasing malondialdehyde (MDA) levels, leading to a notable yield decline. In contrast, inoculation with Streptomyces strains alleviated these adverse effects, with the combined inoculant (Act12+D74) group demonstrating the most significant improvement. Chlorophyll content increased by up to 32.60%, Fv/Fm improved by 43.07%, and antioxidant enzyme activities were enhanced, with superoxide dismutase (SOD) activity increasing by 19.32% and peroxidase (POD) activity by 75.44%. Meanwhile, MDA levels were reduced by 61.61%. The proline content in the combined inoculant group increased by 90.44% at the jointing stage and the soluble protein content increased by 60.17% at the heading stage. Furthermore, it improved the yield by 26.19% by increasing both effective spikes and grains per spike. For the first time, this study revealed the synergistic effects of Act12 and D74 in enhancing photosynthesis, strengthening antioxidant defenses, and optimizing osmotic regulation under drought conditions. These findings provide a theoretical basis for developing environmentally friendly drought management strategies and highlight the potential applications of this inoculant in sustainable agriculture.
Corn or Soybean Oil as the Sole Carbon Source for Polyhydroxybutyrate Production in a Biofuel Biorefinery Concept
Authors: Matte Borges Machado Clara , Porto de Souza Vandenberghe Luciana , de Mello Ariane Fátima Murawski , Soccol Carlos Ricardo
Published: January 2025

The use of polyhydroxybutyrate (PHB) can help diminish fossil chemical dependency because it can partially replace petrochemical plastics due to its biodegradability and similar mechanical properties. However, its production costs are high compared with fossil-based plastics. Alternative carbon sources can be used in the fermentation media because they are renewable and low-cost. Vegetable oils are especially attractive due to their high carbon content, contributing to high production rates per gram of substrate. This work aimed to produce PHB from Cupriavidus necator LPB1421 using either corn or soybean oil as the sole carbon source. Urea was the best nitrogen source, enabling a DCW production of 4.35 g/L (corn oil) and 10.4 g/L (soybean oil). After media optimization, the DCW of corn oil reached 22.13 g/L, with 57.46% PHB accumulation (12.71 g PHB/L), whereas soybean oil led to a DCW of 19.83 g/L, with 54.91% PHB accumulation (10.89 g PHB/L). This media composition was employed in a kinetics assay, revealing similar fermentation parameters among both oils and a yield of 0.2118 g PHB/g for corn oil and 0.1815 g PHB/g for soybean oil. These results open the possibility of integrating PHB production with biofuel manufacturing in a bioethanol/biodiesel biorefinery concept.
In Situ Reactive Extrusion of LDPE Films with Methacrylated Pyrogallol for Antimicrobial and Antioxidant Active Packaging
Authors: Sulistiawan Sharifa Salma , Sadeghi Kambiz , Kumar Ritesh , Kim Dowan , Seo Jongchul
Published: January 2025

Reactive extrusion is a scalable technology for active packaging that promotes food quality and safety. This study investigated the grafting of a methacrylate pyrogallol (PGMC) active agent onto low-density polyethylene (LDPE) through an in situ reactive extrusion process with varying concentrations of PGMC (1, 3, and 5 wt.%). The addition of 5% PGMC increased the tensile strength of pure LDPE from 17.94 MPa to 22.04 MPa. The thermal stability of the samples remained unaffected after PGMC addition, and the grafting process did not interfere with the barrier properties of the LDPE films. Furthermore, 5% PGMC exhibited significant antimicrobial properties, showing 100% and 99.11% reductions in the microbial activity of Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive), respectively. Moreover, the LDPE film with 5% PGMC demonstrated the highest DPPH scavenging effect, reaching up to 85.71%. Therefore, LDPE-g-PGMC films (5%), with antimicrobial and antioxidant properties, have potential applications in non-migratory active packaging.
Highly Conductive Ionohydrogels for Humidity Sensing
Authors: Sun Min-Na , Chen Wen-Yu , Wang Li , Wang Zhi-Gang , Qin Lei , Xie Xu-Ming
Published: January 2025

Polymeric hydrogel materials have excellent electrical conductivity and mechanical properties and will be potentially used in wearable electronic devices, soft robotics, and medical treatment. In this paper, a PAA-Fe3+-IL ionohydrogel (poly(acrylic acid)-Fe3+-ionic liquid ionohydrogel) with excellent mechanical and conductive properties is prepared by simple free radical polymerization. The presence of metal-ligand crosslinking within the ionohydrogel improves the mechanical properties of the hydrogel. When the IL content is 10 wt%, it has the maximum tensile strength and strain. When the ferric ion concentration is 0.3 mol%, the maximum tensile strength is 495.09 kPa. When the ferric ion concentration is 0.1 mol%, the maximum strain is 1151.35%. The tensile behavior of the ionohydrogels is quantitatively analyzed by the viscoelastic model. In addition, free metal ions and anions and cations in IL endowed the hydrogel with a conductivity of 1.48 S/m and a strain sensitivity of 8.04. Thus, the PAA-Fe3+-IL ionohydrogel can be successfully used as a humidity sensor due to the hydrophilic ionic liquid, which can increase the conductivity of the hydrogel by absorbing water. The physical crosslinking density inside the hydrogel is much higher than the chemical crosslinking density, which causes hydrogel dissolution in deionized water by swelling and is conducive to the recycling of the hydrogel. This is a promising material for use in intelligent wearable electronics and as a humidity sensor.
Cellulose-Based Composite Materials for Fresh Water Extraction from Atmospheric Air
Authors: Repin Dmitry , Gablina Mariia , Repina Natalya , Cherednichenko Kirill , Li Wenpeng , Gushchina Yuliiya , Ivanov Evgenii , Melnikov Vyacheslav , Fakhrullin Rawil , Vinokurov Vladimir
Published: January 2025

The fibrous hybrid material was synthesized by suspension radical styrene polymerization on the surface of cellulose microfibers. The resulting material was used to prepare a thermally stable and mechanically strong porous composite matrix that was employed as a carrier for further precipitation of the hygroscopic agents: CaCl2 and 1-butyl-3-methylimidazolium chloride. The obtained composite materials were used to capture atmospheric water at different relative humidity levels and extract fresh water. A composite material containing an ionic liquid (1-butyl-3-methylimidazolium chloride) as a hygroscopic agent demonstrated the best water absorption efficiency and reusability potential.
Structure–Properties Correlations in Novel Copoly(urethane-imide) Films Selectively Destructed Under Thermolysis and Hydrolysis in Alkaline Media
Authors: Didenko Andrei L. , Sukhanova Tatyana E. , Nesterova Anna S. , Vaganov Gleb V. , Lavrentiev Viktor K. , Kabykhno Ilya A. , Grozova Natalia A. , Popova Elena N. , Kamalov Almaz M. , Polotnyanshchikov Konstantin S. , Anokhina Tatyana S. , Borisov I. L. , Kudryavtsev Vladislav V.
Published: January 2025

The paper describes changes in the structure, morphology, mechanical and thermal properties of porous film samples of poly(4,4′-oxidiphenylene)pyromellitimide prepared as a result of selective destruction of urethane blocks in copolymers composed of pyromellitimide blocks and polyurethane blocks. The initial samples of the new composition of statistical copoly(urethane-imide)s (CoPUIs) were prepared via polycondensation methods using pyromellitic dianhydride (PMDA), 4,4′-oxidyaniline (ODA), 2,4-toluylenediisocyanate (TDI), as well as polycaprolactone (PCL) and poly(1,6-hexanediol/neopentylglycol-alt-adipic acid) (ALT) as monomers. The molar ratio of imide and polyurethane blocks in CoPUI was 10:1. The initial films were heated up to 170 °C to complete the polycondensation processes, after which they were subjected to thermolysis and hydrolysis. The thermolysis (thermal degradation) of copolymers was carried out by heating the initial samples to temperatures of 300 °C or 350 °C. Then, the thermolized films were subjected to chemical degradation in hydrolytic baths containing an aqueous solution of potassium hydroxide. As a result, urethane blocks were destroyed and removed from the polymer. The resulting products practically did not contain polyurethane links and, in chemical composition, were practically identical to poly(4,4′-oxidiphenylene)pyromellitimide. NMR and IR spectroscopy, atomic force microscopy, X-ray diffraction, thermogravimetric analysis, differential scanning calorimetry and dynamic mechanical analysis and mechanical properties testing were used to determine the differences in the structure and properties of the initial copolymers and targeted products. The effect of the conditions of destructive processes on the structure, morphology and mechanical properties of the obtained porous polyimide films was determined. From a practical point of view, the final porous films are promising as membranes for filtering aggressive amide solvents at high temperatures.
Adaptability of Electrospun PVDF Nanofibers in Bone Tissue Engineering
Authors: Havlíková Tereza , Papež Nikola , Fohlerová Zdenka , Kaspar Pavel , Dallaev Rashid , Častková Klára , Ţălu Ştefan
Published: January 2025

This study focused on the development of a suitable synthetic polymer scaffold for bone tissue engineering applications within the biomedical field. The investigation centered on electrospun polyvinylidene fluoride (PVDF) nanofibers, examining their intrinsic properties and biocompatibility with the human osteosarcoma cell line Saos-2. The influence of oxygen, argon, or combined plasma treatment on the scaffold’s characteristics was explored. A comprehensive design strategy is outlined for the fabrication of a suitable PVDF scaffold, encompassing the optimization of electrospinning parameters with rotating collector and plasma etching conditions to facilitate a subsequent osteoblast cell culture. The proposed methodology involves the fabrication of the PVDF tissue scaffold, followed by a rigorous series of fundamental analyses encompassing the structural integrity, chemical composition, wettability, crystalline phase content, and cell adhesion properties.
Synthesis, Characterization, and Roles of Vacancy Defects in Polymer and Graphitized Carbon Nitride Photocatalysts: A Comprehensive Review
Authors: Pundi Arul , Chang Chi-Jung
Published: January 2025

Vacancy defect graphitic carbon nitride (g-C3N4) and conjugated polyimide (PI) polymer photocatalysts have become increasingly recognized as metal-free photocatalysts featuring an appropriate bandgap. The narrow absorption spectrum of visible light and the rapid recombination rate of the photoexcited charge carriers in PI polymers and g-C3N4 impede its photocatalytic performance. The presence of oxygen vacancies (OVs) in PI polymer photocatalysts, as well as nitrogen vacancies (NVs) and carbon vacancies (CVs) in g-C3N4, can significantly enhance the migration of photogenerated electrons. Adding vacancies to improve the electronic structure and band gap width can greatly enhance the photocatalytic efficiency of PI polymers and g-C3N4. Defect engineering is important for increasing the photocatalytic ability of PI-polymer and g-C3N4. There remains a notable absence of thorough review papers covering the synthesis, characterization, and applications of vacancy-rich PI-polymer and g-C3N4 in photocatalysis. This review paper examines the roles of OVs in PI-polymer, NVs, and CVs in g-C3N4 and thoroughly summarizes the preparation approaches employed before and after, as well as during polymerization. This review scrutinizes spectroscopic characterization techniques, such as EPR, XPS, PAS, XRD, FTIR, and NMR, for vacancy defect analysis. We also reviewed the role of vacancies, which include light absorption, photogenerated charge carrier separation, and transfer dynamics. This review could serve as a comprehensive understanding, a vacancy-engineered design framework, and a practical guide for synthesizing and characterizing.
Enhancing Thermal Performance of Vertical Ground Heat Exchangers Through a Central Borehole Removal Design
Authors: Aljabr Ahmad
Published: January 2025

The high initial cost of ground heat exchanger (GHE) systems, particularly in applications with significant annual building thermal load imbalances, remains a major barrier to their adoption. In traditional rectangular grid patterns of boreholes, thermal saturation in cooling-dominated buildings mainly affects the central zone, rendering central boreholes less effective. This study investigates an innovative approach to enhance the thermal performance of vertical GHEs by removing these central boreholes using the pygfunction Python package. The central borehole removal design (CBRD) was implemented across various building thermal loads and ground conditions, resulting in reduced borehole interaction and a substantial decrease in total GHE length. Specifically, the CBRD approach achieved up to 51% savings in total GHE length compared to traditional rectangular grid patterns, significantly lowering the initial cost without additional expenses. Although energy consumption savings over a 30-year period were modest (up to 2.2%), the initial cost savings were substantial. Further optimizations indicated that additional reductions in borehole length could be achieved by removing boreholes beyond the central ones, while still maintaining the maximum entering fluid temperature (EFT). Yet, additional optimizations are needed as achieving optimal configurations requires detailed information on factors such as available land area and drilling depth limits, which are site-specific.
Combined Solar Air Source Heat Pump and Ground Pipe Heating System for Chinese Assembled Solar Greenhouses in Gobi Desert Region
Authors: Zhang Gaoshang , Wu Letian , Guo Shenbo , Yue Qiuxing , Sun Xiaoli , Shi Huifeng
Published: January 2025

Chinese Assembled Solar Greenhouses (CASGs) in the Gobi Desert region face significant diurnal temperature variations, with excessively high temperatures during the day and low temperatures at night, which adversely affect crop growth. Traditional temperature regulation technologies are hindered by high energy consumption, high costs, and severe pollutants. To address these issues, this study designed a heating system suitable for CASGs in the Gobi Desert region, integrating solar air source heat pump technology with underground pipe systems. The power consumption and performance of the system were assessed by comparing temperature and humidity in an experimental greenhouse (with the system), a control greenhouse (without the system), and outdoor environments under various typical climate conditions. The results indicated that the system exhibited excellent performance in both daytime heat absorption and nighttime heat release. Specifically, during operation, the maximum daytime temperature in the experimental greenhouse was reduced by up to 5 °C, while the minimum nighttime temperature increased by up to 8 °C, effectively preventing crop frost damage. The system achieved heat absorption rates of 14 to 16 KJ s−1 and heat release rates of 36.5 to 37.5 KJ s−1, with average coefficients of performance (COP) of 4.33 and 4.81. Compared to traditional heating methods using coal, gas, and electricity, the system reduced energy consumption by 84.7%, 81.3%, and 79.1%, respectively, and decreased greenhouse gas emissions by 8.24 t, 6.52 t, and 5.67 t, respectively. This system exhibits outstanding thermal efficiency, energy savings, and environmental benefits, while also showing promising economic benefits with a payback period of four years, providing a reliable heating solution for CASGs in the Gobi Desert region.
Geometrical Evaluation of an Overtopping Wave Energy Converter Device Subject to Realistic Irregular Waves and Representative Regular Waves of the Sea State That Occurred in Rio Grande—RS
Authors: Paiva Maycon da Silveira , Mocellin Ana Paula Giussani , Oleinik Phelype Haron , dos Santos Elizaldo Domingues , Rocha Luiz Alberto Oliveira , Isoldi Liércio André , Machado Bianca Neves
Published: January 2025

Among the various potential renewable energy sources, sea waves offer significant potential, which can be harnessed using wave energy converter (WEC) devices such as overtopping converters. These devices operate by directing incident waves up a ramp into a reservoir. The water then passes through a turbine coupled with an electrical generator before returning to the ocean. Thus, the present study deals with the geometrical evaluation of an overtopping WEC, where the influence of the ratio between the height and length of the device ramp (H1/L1) on the amount of water mass (M) that enters the reservoir was investigated. Numerical simulations were performed using ANSYS-Fluent software, 22 R1 version, to generate and propagate realistic irregular (RI) waves and representative regular (RR) waves found in the coastal region of the municipality of Rio Grande, in the state of Rio Grande do Sul, southern Brazil. Consequently, through constructal design, the optimal WEC geometry for both wave approaches were identified as the same, where (H1/L1)o=0.30. Thus, considering the RI waves, M= 200,820.77 kg was obtained, while, considering the RR waves, M= 144,054.72 kg was obtained.
Operation Optimization Model of Regional Power Grid Considering Congestion Management and Security Check in Complex Market Operation Environment
Authors: Li Yunjian , Zhang Lizi , Cong Ye , Chen Haoxuan , Zhang Fuao
Published: January 2025

Security checks are essential for ensuring the safe operation of the regional power grid (RPG) and the smooth functioning of the electricity spot market (ESM). Currently, China’s RPG operating environment encompasses a complex mix of centralized ESM, decentralized ESM, and planned power generation. This complexity has led to increasingly severe RPG congestion issues. To address this, this paper introduces a security check mechanism design and operational optimization approach tailored for RPGs in complex markets, with a focus on congestion management. Firstly, the paper elaborates on the practical foundations, unique constraints, and requirements for security checks and congestion management during the RPG’s operational mode transitions. Secondly, it outlines the principles underlying the security check mechanism and presents a framework for RPG security checks and congestion management. Through a comparative analysis of three different programs, including their advantages, disadvantages, and applicable scenarios, the paper provides an optimal program recommendation. Building on this, the paper develops an operational optimization method that incorporates congestion management for each of the three security check and congestion management programs. Lastly, an IEEE-39 node test system is simulated to validate the effectiveness of the proposed programs. The mechanism and simulation analysis results show that Program 3, based on market mechanisms, has theoretical and practical advantages over Program 1 (based on multiple adjustments) and Program 2 (based on dispatch plans) for congestion management. Under the same line congestion situation, Program 1 requires two adjustments to relieve the line congestion, while Program 2 and Program 3 can solve the problem with just one optimization adjustment, and the congestion management effect of Program 3 is more obvious and superior.
Can Madhyamaka Support Final Causation? ‘Groundless Teleology’ in Mahāyāna Buddhism, C.S. Peirce, and Chaos Theory
Authors: Berger Jesse R. A.
Published: January 2025

One recurrent criticism of the Madhyamaka doctrine of emptiness (śūnyatā) is its equation with a potential axiological nihilism that undermines, inter alia, the telos of Buddhist practice. Here, I speculate that Madhyamaka non-foundationalism could be compatible with the naturalized teleology of C.S. Peirce. In brief, Peirce argues on pragmatic grounds that the ‘final cause’ of events does not refer to a predetermined finis ultimis or summum bonum with any ‘intrinsic nature’ (‘svabhāva’). Rather, a final cause is a general continuum of lawfulness (‘Third’/future) that mediates between indeterminate possibility (‘First’/present) and determinate actuality (‘Second’/past). Therefore, while a continuum of ‘purposiveness’ is a rational precondition for all temporal events, its futural significance means it can only ever be asymptotically realized; indeed, the constitutively general form of each ‘final’ cause is, practically speaking, fundamentally vague and open-ended to some degree. Finally, I show that the so-called strange attractors of dynamical systems theory provide an imperfect model for this naturalized ‘groundless teleology’.