bluuebunny/crossref_metadata_2025 huggingface dataset:
Dietary Taurine Improves Growth Performance and Intestine Healthy via GSH/GSSG Antioxidant System and Nrf2/ARE Signaling Pathway in Weaned Piglets
Authors: Wang Lingang , Feng Fu , Chu Yunyun , Tang Wenjie , Jiang Liwen , Chen Chen , Qiu Yibin , Hu Zhijin , Diao Hui , Tang Zhiru
Published: July 2023

Early weaning of piglets caused excessive concentration of free radicals, which would oxidize and damage the intestinal tract of piglets, resulting in dyspepsia, low growth, diarrhea, microbiota disorders and intestinal infections. This research studied on effect of different levels of taurine in diets on intestinal oxidative damage in early weaned piglets. A total of 48 weaned piglets (6.71 ± 0.73 kg) of 28-day-old crossbred "Duroc × Landrace × Yorkshire" were assigned to 4 groups with 12 replicates per group and 1 pig per replicate: piglets were fed a basal diet with 0.0% (CON), 0.2% taurine (L-Tau), 0.3% taurine (M-Tau), or 0.4% taurine (H-Tau). The animal experiment lasted for 30 days. With the increase of dietary taurine dosage, the activity of antioxidant enzymes including superoxide dismutase（SOD）,glutathione peroxidase（GPX）,catalase（CAT） and peroxidase（POD） were up-regulated (Linear, P < 0.05), the mRNA abundance of antioxidant enzymes including SOD1, SOD2, GPX1 and heme oxygenase-1（HO-1）were up-regulated (P < 0.05), glutathione (GSH) concentrations was up-regulated (Linear, P < 0.05) , nitric oxide (NO), malondialdehyde (MDA) and oxidized glutathione (GSSG) concentrations were down-regulated (Linear, P < 0.05);The villus height (VH) and villus height/ crypt depth (VH/CD) of jejunum and ileum were increased (Linear, P < 0.05); The expression of tight junction proteins including zonula occludens protein 1(ZO-1), occludin (OCLN) and closure protein 1(CLDN-1) in the jejunum mucosa were up-regulated (Linear, P < 0.05), the mRNA abundance of ZO-1, OCLN and CLDN-1 were up-regulated (P < 0.05),and the D-lactose (D-LA) content in blood was down-regulated (Linear, P < 0.05); the colonization abundance of Firmicutes was increased (Linear, P < 0.05), while Proteobacteria and Spirochaetota was decreased (Linear, P < 0.05); The diarrhea index decreased (Linear, P < 0.05), while the feed conversion rate increased (Linear, P < 0.05). Collectively, supplementation of 0.3% and 0.4% taurine in feed can significantly alleviate intestinal oxidative damage by enhancing antioxidant capacity of piglets, and improve feed utilization of piglets after repairing intestinal damage.
Transfection Models to Investigate Plasmodium vivax-Type Dormant Liver Stage Parasites
Authors: Voorberg-van der Wel Annemarie , Zeeman Anne-Marie , Kocken Clemens
Published: July 2023

Plasmodium vivax causes the second highest number of malaria morbidity and mortality cases in humans. Several biological traits of this parasite species, including the formation of dormant stages (hypnozoites) that persist inside the liver for prolonged periods of time, present an obstacle for intervention measures and create a barrier for the elimination of malaria. Research into the biology of hypnozoites requires efficient systems for parasite transmission, liver stage cultivation and genetic modification. However, P. vivax research is hampered by the lack of an in vitro blood stage culture system, rendering it reliant on in vivo derived, mainly patient material, for transmission and liver stage culture. This has also resulted in a limited capability for genetic modification, creating a bottleneck in investigations into the mechanisms underlying the persistence of the parasite inside the liver. This bottleneck can be overcome through optimal use of the closely related and experimentally more amenable nonhuman primate (NHP) parasite Plasmodium cynomolgi as model system. In this review we discuss the genetic modification tools and liver stage cultivation platforms available for studying P. vivax persistent stages and highlight how their combined use may advance our understanding of hypnozoite biology.
Exploring the Effects of Computer and Smart Device-assisted Learning on students’ achievement: Empirical Evidence from Korea
Authors: Lee Hojun , Kim Young-sik
Published: July 2023

Computer and Smart Device-assisted Learning (CSDL) has gained increasing attention from educational researchers and practitioners in recent years. However, it remains controversial whether student can benefit from CSDL and what moderator could affect the impact of CSDL. Within the specific context of Korea, where the interest in digital education is steadily increasing, the number of empirical studies exploring the causal effect of CSDL remains relatively scarce. The primary objective of this empirical study was to investigate the impact of CSDL on students' academic achievement in Korea. To achieve this objective, a two-way fixed effect model was employed, utilizing a panel dataset spanning three years derived from the 'Korean Education Longitudinal Study 2013'. The findings revealed a significant positive impact of CSDL on students’ Mathematics achievement. Notably, higher income levels, increased availability of computer resources provided by schools, and the implementation of more individualized education were identified as factors that moderate the effect of CSDL on students' achievement levels in Korean and English subjects. These findings underscore the need for an approach that optimizes the educational benefits of CSDL by considering subject-specific characteristics. Furthermore, this study highlights the importance of allocating educational resources, such as computers and smart devices, and integrating individualized educational activities within the classroom environment.
Experimental Study on Sansivera Composite Fibers Against the Administration of Alkaline NaOH (Sodium Hydroxide)
Authors: Kurniawan Teguh Tri , Widodo Edi
Published: July 2023

This study aims to analyze sansivera fiber-reinforced composite materials with a polyester resin matrix and treat sansivera fibers against alkaline NaOH liquids with levels of 4%,7%,10%,13% and 15%.in this study for experimental design using the hand lay-up method,which aims to analyze experimental data,and is also used to determine the minimum number of experiments that can be obtained and provide infotmation on factors that influence parameters.The experiments were carried out through 1)composite manufacturing method,2)immersion with alkaline NaOH,3)fiber tensile test method,4)microstructure test method,5)composite tensile test methods.the results of research on tensile test of fibers from the soaking process with alkaline NaOH 4%,7%,10%,13% and 15%,get the best results in the 15% NaOH alkaline immersion process. From results of the tensile test the fibers will be formed into 7 composite specimens, from the composite tensile test the maximum tensile strength is obtained on specimen 5 with 40% treatment of 45,71 N/mm2 and the highest elongation obtained on specimen 4 with 30% treatment of 3,95mm.
Comparative assessment of the DNA damage caused in cumulus cells by Corneumon®, Gonal-F®, Pergoveris® and Puregon® in women undergoing assisted reproduction techniques, and its impact on reproductive outcomes
Authors: ALCALÁ-SÁNCHEZ Ximena , CUAPIO-PADILLA PEDRO , SALAZAR-LÓPEZ CARLOS , RODRÍGUEZ RICARDO , TETELTITLA MARIO , BAHENA IVAN , BETANCOURT MIGUEL , CASAS EDUARDO , CASILLAS FAHIEL , LÓPEZ ALMA , BONILLA EDMUNDO
Published: July 2023

Assisted reproduction technology (ART) is performed with the support of controlled ovarian hyperstimulation (COH) to increase the number of oocytes retrieved, thus improving the success rates of in vitro fertilization (IVF). Several recombinant human follicle stimulating hormone (rhFSH) are used in ART, and significant research has been undertaken to evaluate the effectiveness and safety of the various comercially available rhFSH. Due to their close bio-dynamic relationship with the oocyte, cumulus cells (CCs) have been proposed as biomarkers of oocyte quality. The aim of the present study was to perform a comparative assessment of the DNA damage caused in CCs of women undergoing ART by four widely used rhFSH in IVF protocols, Corneumon®, Gonal-F®, Pergoveris® and Puregon®, and to analyze the impact of the CCs DNA damage on several IVF outcomes. Comet assay results show that the tested rhFSH caused variable and statistically significant levels of CCs DNA damage (Corneumon® < Pergoveris® < Gonal-F® < Puregon®). Analysis of the reproductive outcomes show that the groups with lower CCs DNA damage (Corneumon® and Pergoveris®) had higher number of oocytes retrieved, fertilization rates, and number of high-quality embryos suitable for IVF/vitrification, than the groups with higher CCs DNA damage (Gonal-F® and Puregon®), however the differences between the groups were not statistically significant. In addition, the Pearson’s correlation between fertilization rates and CCs DNA damage was statistically significant, r = −0.98, p = 0.017. These results support CCs DNA damage as an index of the effectiveness of rhFSH and human oocyte competence.
MPPT techniques for PV systems: A comprehensive review of current trends and recent advances in AI
Authors: Boubaker Olfa
Published: July 2023

Over the past decades, solar photovoltaic (PV) energy has been the most valuable green energy. It is renowned for its sustainability, environmentally friendly nature, and minimal maintenance costs. Several methods aiming to extract the highest photovoltaic energy are found in the vast literature. The aim of this review is to focus on current trends and the most recent advances in the field. A “Scopus” bibliographic survey is conducted around survey and research articles published over the past three years (2019–2022) according to a predefined selection method. Over the selected works, different taxonomies of maximum power point tracking (MPPT) approaches are found. The list of associated performance criteria is also established and current trends in the field are well identified. This survey paper could be a useful reference for researchers and companies concerned by the sustainable development goals (GSD) for clean energy production and climate change.
Comparative transcriptome analysis of waterlogging induced adventitious root and control root of Mentha arvensis
Authors: Pandey Durgesh Kumar , Upadhyay Swati , Phukan Ujjal J. , Shukla Rakesh
Published: July 2023

Waterlogging a significant problem to plant growth in the areas facing the waterlogged condition of the soil. In our previous findings, Mentha arvensis was shown to adapt better in waterlogging conditions by initiating the early onset of adventitious root formation. In this study, we analysed the adventitious root's transcriptome and compared it with the control root. Analysis of differentially expressed genes in adventitious root in comparison with the control root were grouped into four functional categories, i.e., carbohydrate metabolism, antioxidant activity, hormonal regulation, and regulation of transcription factors that may majorly contribute to the development of adventitious roots. Differential expression of the upregulated and uniquely expressing twenty-four transcripts in adventitious roots was validated using q-RT PCR. The biochemical parameters total carbohydrate, total protein contents and antioxidant enzymes such as nitric oxide, catalase, and superoxide dismutase (SOD) activity were enhanced in the adventitious root. These identified transcripts upregulated or uniquely expressed in the adventitious root will be useful to understand their role in the waterlogging-induced emergence and development of adventitious roots, leading to tolerance towards waterlogging stress in plants.
Epileptic seizure and electroencephalographic characteristics of adult COVID-19 inpatients during the Omicron outbreak in China: a single center retrospective study
Authors: zhang jianfang , zhou jiajia , wu dengchang , gi renjie , zhang xing , wang qi , wang kang
Published: July 2023

Background This retrospective analysis assessed the characteristics of epileptic seizure and continuous electroencephalogram (CEEG) data in hospitalized patients diagnosed with COVID-19 during the Omicron outbreak. Methods CEEG was performed in 28 patients, with 17 showing unexplained altered mental status and 11 suspected of having seizures. Demographic and clinical variables, imaging results, outcomes, and comorbidities were collected. Results In total, 1,405 patients with COVID-19 infection were admitted during the study period. The proportion of patients who required intensive care unit (ICU) care and the in-hospital mortality was 16.3% and 7.8%, respectively. Among patients who underwent CEEG monitoring, 11 were in ICU, and 17 were in regular wards. Of these, 8 patients (28.5%) had severe COVID-19, whereas 6 had acute neuroimaging findings. EEG findings were not specific, with 7 patients (25%) having normal EEG results. Furthermore, 11 (39.3%) had benign EEG alterations, 6 (21.4%) had malignant, and 4 (14.3%) had highly malignant. Six patients exhibited epileptiform abnormalities, including 1 with a prior epilepsy history. Moreover, 4 patients experienced electrographic seizures, with 2 manifesting as epilepsia partialis continua and 2 as nonconvulsive status epilepticus. Periodic and rhythmic patterns were observed in 2 patients, spike-and-wave in 1 patient and generalized rhythmic delta activity in another patient. EEG attenuation without reactivity was seen in 4 patients. Conclusions Seizures can manifest as early symptoms in individuals infected with the Omicron variant of COVID-19. Despite the increased contagiousness associated with Omicron, we observed a higher prevalence of normal EEG results. This suggested that the Omicron variant may be associated with a lower likelihood of causing encephalitis or encephalopathy compared to other variants.
Lower creatinine to cystatin C ratio is associated with an increased risk of MAFLD: A cross-sectional and prospective study of 368,634 UK Biobank participants
Authors: Wang Jiaren , Zeng Lin , Hong Chang , Cui Hao , Zhu Hongbo , Li Qimei , Li Yan , Li Ruining , He Jingzhe , Zhu Hong , Liu Li , Xiao Lushan
Published: July 2023

Background & Aims: Metabolic associated fatty liver disease (MAFLD) affects about a quarter of the world's adults, and screening out the high-risk population with at an early stage is a challenge. As a sarcopenia index, the relationship between creatinine to cystatin C ratio (CCR) and MAFLD remains unclear. This cross-sectional, prospective study aimed to explore the relationship between CCR and MAFLD.
Methods: This study included 368,634 participants from the UK Biobank for cross-sectional and prospective analyses. MAFLD was diagnosed according to international expert consensus, and mild and severe liver fibrosis were defined by FIB-4. Univariate and multivariate logistic regression analyses were used to calculate odds ratios (ORs) to evaluate the association between CCR levels and MAFLD. Cox regression models were used to determine the association between CCR and MAFLD-related severe liver disease (SLD).
Results: We grouped the study participants according to CCR tertiles. In cross-sectional and prospective analyses, participants in CCR tertile 1 had the highest MAFLD risk (OR 1.075, 95%CI 1.058-1.093, P<0.001, and OR 1.340, 95%CI 1.077-1.669, P=0.009). In males, CCR was significantly associated with severe liver fibrosis (OR 1.657, 95%CI 1.476-1.859, P<0.001). Cox analyses showed that participants in CCR tertile 1 had the highest risk of MAFLD-related SLD (HR 4.23, 95%CI 3.54-4.05, P<0.001), and the SLD cumulative incidence during follow-up was obviously higher than others.
Conclusion: Our results indicated that lower CCR was significantly associated with higher risk of MAFLD. Moreover, CCR can predict the risk of MAFLD-related SLD and severe liver fibrosis, which can potentially be used as a large-scale non-invasive tool to dynamically monitor the occurrence and development of MAFLD.
Association between blood glucose control and risk factors of latent tuberculosis in the population with diabetes: Results from the National Health and Nutrition Examination Survey (NHANES) 2011–2012
Authors: Feng Yanmei , Tang Dezhu , Tang Li , Chen Xi , Yi Guangzhao , Jia Jinwei , Wang Pu , Guo Rui
Published: July 2023

Background
Diabetes has been conferred an increased risk of latent tuberculosis infection. However, the level of blood glucose control and associated risk factors for latent tuberculosis infection in diabetes population is poorly understood. This study aimed to determine the relationship between blood glucose control and the risk factors for latent tuberculosis infection (LTBI) in individuals with diabetes mellitus (DM) using the National Health and Nutrition Examination Survey datasets.
Methods
The association between blood glucose control and LTBI in patients with DM were investigated with logistic regression analysis. Additionally, covariates such as gender, age, education level, and ethnicity were also included in the analysis.
Results
A total of 2489 patients were included in this study, of which 76.3% were Non-Hispanic White, Black and Asia. 231 participants were positive with LTBI. Multivariable analysis demonstrated that the poor blood glucose control exhibited a 150% greater risk of LTBI in DM population. Besides, female gender in DM population would less likely occurring LTBI in DM population (AOR: 0.71, 95%CI: 0.55–0.91, P = 0.006). Meanwhile, with age increased, the risk of LTBI gradually raised. Comparing to patients aged 18 to 45 years old, the prevalence of LTBI in elderly more than 70 years old was much higher (AOR: 3.16, 95%CI: 2.11–4.74, P < 0.001). Furthermore, the ethnicity was also associated with LTBI in DM population. Non-Hispanic White and Black population with DM were less likely developing LTBI.
Conclusions
The findings of this study highlight the association between poor blood glucose control and the prevalence of LTBI in patients with DM. Furthermore, male, aging, and ethnicity were independent risk factors for LTBI in the DM population.
Machine Induced Dynamic Field Responses of Group Pile with Different Pile Arrangements
Authors: Kumar Sumit , Choudhary Shiva Shankar , Burman Avijit
Published: July 2023

The main objective of the current investigation is to study the dynamic behaviour of a 3-pile group with two different arrangements of pile setups under coupled (horizontal and rocking) excitations. To accomplish this objective, machine-induced field excitation tests are conducted on small-scale hollow steel piles. The 3-pile group is driven into the ground in a triangular arrangement with 3d spacing. Two different soil-pile setups, i.e., Pile Group-I and Pile Group-II, are created based on dynamic force directions. In the case of Pile Group-I, the forces are applied to the direction of the median of the triangle, and for Pile Group-II, the forces are applied to other directional loads. From the test results, it is found that the resonant peaks of horizontal and rocking amplitudes for Pile Group-I are lower than Pile Group-II. In the case of resonant frequencies, the values of Pile Group-I are observed to be the same or a little bit higher as compared to Pile Group-II. It is found that the dynamic soil-pile-soil interaction effect is more prominent for Pile Group-II than for Pile Group-I. For numerical investigation, the continuum approach method is utilised with the inclusion of a dynamic group interaction factor to predict the dynamic coupled responses in terms of frequency and amplitude for these two soil-pile setups. To understand the behaviour of pile groups, boundary zone parameters and variations of impedance parameters (stiffness and damping) with operating frequencies are also measured using this theoretical approach.
Contamination reduction of vegetable oil refinery wastewater using innovative acid and basic chemical flotation processes
Authors: Hartal Oussama , Souabi Salah , Chatoui Mohamed , Ettaloui Zineb , Madinzi Abdelaziz , Rifi Safaa Khattabi , Kurniawan Tonni Agustiono , Anouzla Abdelkader
Published: July 2023

Vegetable oil refineries produce a large amount of wastewater, creating several environmental problems that threaten aquatic life. These wastewaters present significant challenges for treatment techniques due to their high and complex pollution characteristics. This study presents the application of flotation processes in acid medium or basic medium of vegetable oil refinery wastewater to reduce turbidity, oil, grease, chemical oxygen demand (COD), and phosphorus total, polyphenols, nitrates, phenolic compounds measured at 254 nm and color. Application of acid flotation reduced turbidity (96.9%), COD (79%), nitrate (97.8%), total phosphorus (99.8%), polyphenol (86.6%), absorbance at 254 nm (90.8%), oil and grease (98.5%) and color (96.6%). However, the application of basic flotation showed a reduction in turbidity (25.7%), COD (37.5%), nitrates (91.6%), total phosphorus (88%), polyphenols (66.3%), absorbance at 254 nm (64.7%), oil and grease (68.8%) and color (73.9%). Therefore, this flotation process in an acidic or basic medium can be considered among the most effective processes for reducing wastewater pollution from vegetable oil refineries.
‘Sand Types’: A new physico-chemical classification of sand and its mapping using conditional strings in ArcGIS10.x
Authors: AC Dinesh A.C. , NV Nisha N.V. , Pillai Rachna
Published: July 2023

The economic viability of sand in the construction industry depends on its size, shape, shell content, etc. It is imperative to understand the proportions of sand sub-classes and shell content in a sand sediment while planning exploration and mining. The existing sediment classifications (Particle Size Distribution Classifications) based on Sand-Silt-Clay (SSC) or Gravel-Sand-Mud (GSM) of different authors have not addressed these issues and it remains as a gap area till now. Hence, a new physico-chemical classification (‘Sand Types’) of sand that falls in the ‘sand fields’ of Shepard (1954) SSC and Folk (1954) SSC is being proposed. The freeware program ‘Sand Types’ developed for plotting types of sand in the ternary diagram helps to plot the data in a triangular diagram and identify their physico-chemical properties. The program also offers conditional strings that are prepared based on the proposed classification and are coupled with that of SSC classifications. These conditional strings work in ArcGIS 10.x platform for the preparation of sediment distribution maps with meaningful information. This would enable construction industry/end-users to plan and execute detailed exploration and mining programs at great ease.
In Situ Brain Tumor Detection Using the Raman Spectroscopy Sentry System – Results of a Multicenter Study
Authors: Leblond Frédéric , Ember Katherine , Dallaire Frédérick , Plante Arthur , Sheehy Guillaume , Guiot Marie-Christine , Agarwal Rajeev , Yadav Rajeev , Douet Alice , Selb Juliette , Tremblay Jean-Philippe , Dupuis Alex , Marple Eric , Urmey Kirk , Rizea Caroline , Harb Armand , McCarthy Lily , Schupper Alexander , Umphlett Melissa , Tsankova Nadejda , Hadjipanayis Constantinos , petrecca Kevin
Published: July 2023

Safe and effective brain tumor surgery aims to remove tumor tissue, not non-tumoral brain. This is a challenge since tumor cells are often not visually distinguishable from peritumoral brain during surgery. To address this, we conducted a multicenter study testing whether the Sentry System could distinguish the three most common types of brain tumors from brain tissue in a label-free manner. The Sentry System is a new real time, in situ brain tumor detection device that merges Raman spectroscopy with machine learning tissue classifiers. Nine hundred and eighty-five in situ spectroscopy measurements and colocalized tissue specimens were acquired from 67 patients undergoing surgery for glioblastoma, brain metastases, or meningioma to assess tumor classification. The device achieved diagnostic accuracies of 91% for glioblastoma, 97% for brain metastases, and 96% for meningiomas. These data show that the Sentry System discriminated tumor containing tissue from non-tumoral brain in real time and prior to resection.
Chaotic spindles causes abnormal chromosome behavior in an interspecific sexual hybrid of Urochloa
Authors: Sales Gabriel Luiz de Melo , Valle Cacilda Borges do , Mendes Andréa Beatriz Diverio
Published: July 2023

Intra- and interspecific hybridization in the genus Urochloa is complex due to apomixis and polyploidy. Such barriers can be overcome by crossing apomictic tetraploid plants as pollen donors with artificial tetraploid sexual plants as female parents. Another barrier is the incompatibility between the genomes of the different species used in hybridization. Since the meiotic behavior is a good indication of affinity between genomes in a hybrid, the present study analyzed the meiotic behavior of a sexual polyploid interspecific hybrid of Urochloa from the breeding program at Embrapa Beef Cattle. In addition to the segregational abnormalities associated with polyploidy, cytological studies revealed that, after diakinesis, chromosomes presented an abnormal arrangement in the metaphase plate. In 31.33% of meiocytes, chromosomes were organized into more than one plate, forming up to four metaphase plates. Another abnormality observed in 5.72% of the cells was the lack of convergence of chromosomes to the equatorial plate and consequent abnormal anaphase segregation. These abnormalities led to the formation of several nuclei within the same myocyte then separated by an irregular cytokinesis pattern, forming tetrads with micronuclei, microcytes and polyads. This pattern of behavior seriously compromises the formation of viable gametes, which may later affect the generation of fertile hybrids thus impairing the use of this hybrid as a female parent.
Association of renin–angiotensin–aldosterone system inhibitors with paclitaxel-induced peripheral neuropathy
Authors: Ihara Yasutaka , Sawa Kenji , Imai Takumi , Kimura Tetsuya , Otani Miho , Kawai Ryota , Takatori Shingo , Shintani Ayumi
Published: July 2023

Purpose
Chemotherapy-induced peripheral neuropathy (CIPN) has been reported to reduce patients’ quality of life and impair cancer treatment by causing anticancer drug withdrawal or interruption. However, there are currently no effective methods for the prevention of CIPN. Renin–angiotensin–aldosterone system (RAAS) inhibitors may be associated with a reduced risk of developing oxaliplatin-induced peripheral neuropathy, and it would be valuable to examine whether they have the same effect on CIPN caused by other anticancer drugs. Our study explored the potential preventive effects of RAAS inhibitors on preventing paclitaxel-induced peripheral neuropathy (PIPN).
Methods
An exploratory cohort study was conducted using commercially available administrative claims data on lung cancer patients treated with paclitaxel-based chemotherapy. Cumulative paclitaxel doses, RAAS inhibitor prescriptions, and incidences of PIPN were identified using patient medical records. Fine–Gray analyses with death as a competing risk were performed. A propensity score approach was applied to address the problem of confounding.
Results
Patients with lung cancer who received paclitaxel-based chemotherapy were classified into the RAAS inhibitor group (n=1,320) and non-RAAS inhibitor group (n=4,566). The doses of RAAS inhibitors in our study were similar to those commonly used to treat hypertension. The PIPN incidence was significantly lower in the RAAS inhibitor group than in the non-RAAS inhibitor group (sub-distribution hazard ratio, 0.871; 95% confidence interval, 0.789–0.961). The result was consistent in various sensitivity analyses and important subgroup analyses.
Conclusions
RAAS inhibitors at doses commonly used for hypertension were associated with a reduced incidence of PIPN in patients with lung cancer.
Ursolic acid inhibits the growth of colorectal cancer cells through regulating the PKN2/Akt/ERK pathway by targeting SNTB1
Authors: Lu Wen , Liu Liya , Lin Xiaoying , Liu Huixin , Shen Aling , Wei Lihui , Sferra Thomas J. , Chen Youqin , Li Li
Published: July 2023

Background
Colorectal cancer (CRC) is one of the most highly malignant tumors. Ursolic acid (UA) has been identified to exerts anti-CRC effects through the regulation of multiple genes and signaling pathways. Syntrophin beta 1 (SNTB1) has been implicated to have the potential as a new prognostic factor and therapeutic target for CRC. We wondered whether UA exert anti-CRC effects by regulating SNTB1 expression.
Methods and Results
CCK8 assay and colony staining was conducted to determine the cell viability and colony formation of Human CRC cell line HCT116. Lentiviral infection was employed for SNTB1 knockdown and overexpression. Western blot analysis was performed to detect the protein expression. The result shown that UA significantly inhibited cell viability and downregulated SNTB1 expression while upregulating PKN2 expression. It also decreased the ratios of p-Akt/Akt and p-ERK/ERK. UA significantly promoted the decrease of cell viability and colony formation in HCT116 cells with SNTB1 knockdown, the upregulation of PKN2 protein expression, and the downregulation of the p-Akt/Akt and p-ERK/ERK ratios. On the other hand, UA significantly inhibited the increase of cell viability and colony formation in HCT116 cells with SNTB1 overexpression, the downregulation of PKN2 protein expression, and the upregulation of the p-Akt/Akt and p-ERK/ERK ratios.
Conclusion
Take together, UA inhibits CRC cell growth may through regulating the PKN2/Akt/ERK pathway by targeting SNTB1. This study enriches the mechanisms underlying the anticancer effects of UA in CRC and provides new evidence for UA as a potential anti-CRC agent.
Contribution to the Anatomical Nomenclature Concerning Anterolateral Knee Anatomy
Authors: Yilmaz Niymet Pelin Cavdar , Comert Ayhan , Acar Halil İbrahim , Kachlik David
Published: July 2023

Purpose
The aim of present study is to extend the sections of Terminologia Anatomica (TA) concerning anterolateral knee structures and reveal the clinical, educational and sceintific importance of using that newly proposed anatomical terms.
Methods
Anatomical terms were gathered both from anatomical textbooks and journals. Samples were compared with the last addition of the official Latin anatomical nomenclature. Proposed terms have indicated on a black and white picture and cadaveric dissection photographs from archive.
Results
The authors proposed 12 new terms which are often used clinical medicine, textboks and scientific articles. Those terms were summarised and are presented with their definitions and explanations on behalf of constant and variable morphological structures.
Conclusions
There are numerous studies according to anterolateral complex of knee in the literature. Consideration of clinical importance and increasing remarks in literature, the potential of the proposed anatomical terms should be discussed concerning for adding into the next addition of the TA.
A survey of sentiment analysis methods based on graph neural network
Authors: Rad Razieh Abedi , Yamaghani Mohammad Reza , Nourbakhsh Azamossadat
Published: July 2023

Sentiment analysis is an active research field as one of the most popular tasks of natural language processing, which aims to extract valuable information from various social platforms and extensive online texts to process and find people's attitudes in business and advertising, government, economic fields, and even political orientations. Hence, researchers have made many efforts in this field, which mainly refer to traditional approaches based on dictionaries, machine learning, and deep learning models. Graphs as a robust and interpretable data structure have been considered for applications of artificial intelligence models such as machine vision and natural language processing which are used for learning non-structured data like text or images. Although deep learning methods have achieved promising results in this field, due to problems such as assigning indecisive weights and high dimensions in feature extraction stages, they are still a “black box.” Meanwhile, graph neural networks (GNNs) are a particular type of deep neural network that are interpretable and flexible. Their adaptability in solving complex problems in data analysis with a graph structure has made them one of the most efficient methods in the last decade. Considering the large amount of textual information in social media and various online platforms, sentiment analysis or opinion mining aims to help marketing strategies for business owners and awareness of the attitude of public opinion in governments has become one of the crucial issues in today's modern societies. This comprehensive review focuses on GNN-based approaches in sentiment analysis and summarizes the recent state-of-the-art in this area. Also, we discussed their weaknesses and strengths, and challenges on specific datasets. Our goal is to show the development process and the potential of GNN-based approaches in different problems of sentiment analysis compared to previous methods and to help find more effective directions for researchers interested in this field.
Comparison of different surgical treatments for stage II progressive collapsing foot deformity deformity-A Finite element analysis
Authors: Chen Fanglin , Yuan Chengjie , Liang Mian , Xie You , Le Guoping , yang Chengzhi , Chen Renqiang , Chen Kenian , Xu Jian
Published: July 2023

Background: This study analyzed the advantages and disadvantages of different procedures for stage IIA progressive collapsing foot deformity (PCFD) through three-dimensional finite element models.
Methods: Medial column fusion (MCF), medializing calcaneal osteotomy (MCO), lateral column lengthening (LCL), and subtalar joint arthroereisis (SJA) operations were simulated. The maximum pressure on plantar soft tissue, medial column bone, and medial ligaments was compared before and after simulated single-foot weight loading.
Results: The maximum plantar stress of PCFD decreased with MCO and SJA but increased with MCF and LCL. MCF and LCL failed to significantly reduce the stress on the medial column fragments, thereby increasing their stress. Both MCO and SJA relieved medial plantar stress. MCF had no significant effect on stress relief of the medial ligament. MCO, LCL, and SJA were all shown to reduce the pressure on the medial plantar ligament, with LCL having the most obvious effect. All four procedures corrected the arch deformity; however, MCF was not as effective as the other methods. SJA is the best method for restoring arch height and correcting arch deformities. For stage IIA PCFD, isolated MCF failed to reduce pressure on the medial column; however, isolated MCO significantly reduced the pressure on the medial plantar and ligamentous soft tissues while restoring the foot’s arch and correcting the hindfoot valgus.
Conclusion:SJA with type II sinus tarsi implant effectively transferred pressure from the medial plantar tract to the lateral side and restored the arch. Isolated LCL was not found suitable for stage IIA PCFD.
Nomogram development and evaluation for resectable colorectal cancer based on preoperative hemoglobin levels and lymphocyte to monocyte ratio：a retrospective cohort study
Authors: Zhou Jie , Tian Mengjie , Zhang Xiangchen , Xiong Lingyi , Xu Hongli , Yin Zhucheng , Wu Fengyang , Hu Junjie , Liang Xinjun , Wei Shaozhong
Published: July 2023

Background: The impact of lymphocyte to monocyte ratio (LMR) and hemoglobin (Hb) level on the prognosis of solid tumors and hematological malignancies was reported. Therefore, we sought to investigate the prognostic value of LMR-Hb based biomarker in resectable colorectal cancer (CRC).
Methods: Clinical data from 700 CRC patients received radical surgery at Hubei Cancer Hospital from 2013 to 2016 were retrospectively analyzed. To evaluate the connection between clinical outcomes and elements regarding demographics, pathological and laboratory index, we conducted Multivariate Cox regression. A novel nomogram was developed, and its performance was evaluated by its discrimination, calibration, and clinical usefulness.
Results: Kaplan-Meier method demonstrated that poorer overall survival (OS) and disease-free survival (DFS) was associated with higher Hb-LMR Score (P < 0.0001). Multivariate Cox regression illustrated that age at surgery greater than or equal to 65 years, female, the rate of lymph nodes metastasis reaching 0.077 or more, invasion of circumferential resection margin, TNM staging in III-IV, and Hb-LMR score of 2 were independent risk factors for poor OS or DFS. The area under the time-dependent receiver operating characteristic curve, the calibration curve along with decision curve analysis were performed and revealed that the nomogram was excellent in discrimination, calibration and efficacy. The concordance index illustrated that the nomogram has more accurate predictive ability for OS and DFS than TNM stage alone.
Conclusion: Based on preoperative Hb and LMR, we successfully developed a novel and easily accessible prognostic score which had a good performance in predicting clinical outcomes in CRC patients.
Skeletal Expansion Via Craniofacial Distraction Osteogenesis Technique In Syndromic Craniosynostosis: Impact On Ophthalmic Parameters
Authors: Lim Chin Kai , Khaliddin Nurliza , Hassan Muhammad Kamil , Hariri Firdaus
Published: July 2023

Background
This study aims to compare the changes in ophthalmic parameters among syndromic craniosynostosis patients who underwent craniofacial skeletal expansion procedures via distraction osteogenesis (DO).
Method
A retrospective study was conducted involving syndromic craniosynostosis patients who underwent surgical expansion via the DO technique from the year 2012 to March 2022. Changes in six parameters which consist of visual acuity, refractive error, optic disc health, intraocular pressure, degree of proptosis and orbital volume were measured objectively pre and post-surgery. For categorical parameters, the Chi-square cross-tab test was done. Paired sample T-test was used for normally distributed variables. Wilcoxon signed-rank test was used for non-normally distributed data.
Results
Visual impairment was present in 21.4% of eyes before surgery and increased to 28.5% post-surgery. Three patients had changes of refractive error post-surgery with one developed hypermetropia, another developed anisometropia and the last had improvement to no refractive error. Two patients had optic disc swelling which was resolved post-surgery. Intraocular pressure changes were inconsistent post-surgery. All patients achieved a significant reduction in the degree of proptosis post-surgery. Orbital volume calculation using computed tomography (CT) scans shows a significant increase in volume post-surgery for all patients.
Conclusion
Our study shows a significant increase in orbital volume post-surgery with a reduction in the degree of proptosis. Optic disc and nerve health improved after the surgery. Changes in terms of visual acuity, refractive error and IOP were inconsistent after the surgical intervention.
Research on Enterprise Financial Risk Warning Based on AutoEncoder-ConvNeXt
Authors: He Yi , Liu Hongjiu , Hu Yanrong , Liu Qingyang
Published: July 2023

This study combines the AutoEncoder and ConvNeXt models to conduct financial risk warning research on 167 Chinese agro-forestry related enterprises. Firstly, a set of 52 indicators was determined by similarity calculation to further discuss the classification method of financial risk. The CRITIC method, combined with grey correlation analysis, fuzzy comprehensive evaluation, and TOPSIS method, was used to score the financial risk of agro-forestry enterprises. Based on this, a self-organizing mapping network was used to classify the financial risk level, and finally, the AutoEncoder-ConvNeXt model was used to predict the financial risk of enterprises. Compared with other models such as ResNet50 and original ConvNeXt, the prediction accuracy of the AutoEncoder-ConvNeXt model was higher at 87.11%, making it better suited for predicting the financial risks of listed companies.
Nicotinamide Mononucleotide Versus Nicotinamide Riboside in The Protective Effects of Cisplatin-induced DNA Damage in HeLa Cells
Authors: Qiu Shuting , Zhang Yunheng , Shao Shihan , Zhang Yingying , Yin Jie , Xu Xianrong , Yang Lei , Hong Yu , Yang Jun , Tan Xiaohua , Di Chunhong
Published: July 2023

Background Previous studies have shown that the nicotinamide adenine dinucleotide (NAD+) precursors, nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR), protected against endogenously or exogenously induced DNA damage, however, whether the two compounds have the same or different efficacy against DNA damage is not clear. In the current study, we systematically compared the effects of NMN and NR on cisplatin-induced DNA damage in HeLa cells. Methods The viability of cisplatin treated HeLa cells with NMN or NR were tested by Trypan blue staining. NMN and NR were added in cells before or after exposed to cisplatin, respectively. Briefly, HeLa cells were pretreated with series doses of NMN or NR (0、0.625、1.25、2.5、5 and 10 mM) for 12 h, and then challenged with 10 µM of cisplatin for the following 12 h; or, HeLa cells were treated by 10 µM of cispaltin for 12 h, and then cultured in medium with 10mM NMN or NR, the cells were harvested at 0, 8, 16, 24 and 32 h later. The DNA damage were assessed by immunofluorescent against phosphor-H2AX (γH2AX) and alkaline comet assay. The intracellular nicotinamide adenine dinucleotide (NAD+) and reactive oxygen species (ROS) were determined by according kit. Results Both NMN and NR could rescued cisplatin-induced cell death in a dose-dependent manner comparably. NMN and NR pretreatment decreased γH2AX levels and shortened comet tail length in a dose-dependent manner, while NR pretreatment exhibiting stronger protective effects than NMN. Although the post-cisplatin administration of NMN and NR also exhibited a protective effect against DNA damage, there were no significant differences between the two compounds. In addition, both NMN and NR can reverse the cisplatin-induced decrease of NAD+ and the generation of ROS, also with no significant difference between them. Conclusion NR is more effective than NMN in maintaining DNA integrity in cisplatin-treated cells.
Mix-net: A Dual-Branch Lightweight Semantic Segmentation Network Based on MRF
Authors: Tan Jingbin , Zhao Xujun , Cai Jianghui , Yang Haifeng , Su Huijuan
Published: July 2023

To enable image segmentation tasks to go beyond microcomputers and similar devices and better adapt to the limited computing and storage capabilities of mobile devices, we have proposed a lightweight semantic segmentation network called Mix-net. Firstly, we designed a Mix Convolution (MC) module for feature extraction and recovery, which significantly reduces the number of parameters in the model while maintaining accuracy. Secondly, to enhance the edge details of various semantics in the images, we simultaneously train two network models. One model focuses on learning the main body details of the images, while the other model is specifically designed for segmentation tasks and is more dedicated to capturing the edges of different semantics. Thirdly, we designed a novel activation function called Mix Rectified Function (MRF), which effectively enhances the accuracy of segmentation tasks and is more suitable for lightweight image segmentation tasks. Additionally, we have employed Group Normalization (GN) to complement MRF for normalization and activation operations, enabling higher accuracy for small-batch segmentation tasks. Finally, our proposed network retains skip-connection structures and incorporates channel shuffling and attention modules to enhance accuracy. We have conducted comparative experiments on the Cityscapes dataset, where Mix-net achieved an Mean Intersection over Union (MIoU) of 81.1% and a smaller model size, utilizing only 1.47M Parameters and 9.93G Floating Point Operations (FLOPs) on a single NVIDIA GeForce RTX3060 graphics card.
Study of a formaldehyde sensor based on cellulose nanocrystals
Authors: Lin Tao , Wang Le , Wei Xiaoyao , Li Jie , Yin Xuefeng
Published: July 2023

Since formaldehyde poses a major threat to human health, it is critical to perform convenient, rapid, and specific formaldehyde detection. Owing to their interesting optical features, iridescent films consisting of chiral nematic liquid crystal phases of cellulose nanocrystals (CNC) have attracted the attention of researchers. As a result, the widebandgap n-type semiconductor SnO2 and CNC, which have stable and outstanding gas-sensitive features as well as high optoelectronic performance, were combined to create biomass-derived chiral nematic composite films. Therefore, a biomass-derived chiral nematic composite film was prepared by combining the wide-band-gap n - type semiconductor SnO2 with stable, excellent gas-sensing characteristics and optoelectronic properties with CNC. Formaldehyde can be integrated into the nematic layer of CNC films and the formaldehyde gas is detected on the CNC. The formed composite film shows yellow, green, and purple structural colors for different formaldehyde concentrations, realizing the recognition by the naked eye of formaldehyde. This produces a red shift in the reflected wavelength and an effective chromaticity jump. This hybrid thin film is expected to advance a new gas-sensing system for rapid and efficient in situ qualitative investigations.
Multi-tissue transcriptomic and serum metabolomic assessment reveals systemic implications of acute ozone-induced stress response in male Wistar Kyoto rats
Authors: Jackson Thomas W. , House John S. , Henriquez Andres R. , Schladweiler Mette C. , Jackson Kimberly MP , Fisher Anna A. , Snow Sam J. , Alewel Devin I. , Motsinger-Reif Allison , Kodavanti Urmila P.
Published: July 2023

Air pollutant exposures have been linked to systemic disease; however, the underlying mechanisms between responses of the target tissue and systemic effects are poorly understood. A prototypic inducer of stress, ozone causes respiratory and systemic multiorgan effects through activation of a neuroendocrine stress response. The goal of this study was to assess transcriptomic signatures of multiple tissues and serum metabolomics to understand how neuroendocrine and adrenal-derived stress hormones contribute to multiorgan health outcomes. Male Wistar Kyoto rats (12–13 weeks old) were exposed to filtered air or 0.8 ppm ozone for 4-hours, and blood/tissues were collected immediately post-exposure. Each tissue had distinct expression profiles at baseline. Ozone changed 1,640 genes in lung, 274 in hypothalamus, 2,516 in adrenals, 1,333 in liver, 1,242 in adipose, and 5,102 in muscle (adjusted p-value < .1, absolute fold-change > 50%). Serum metabolomic analysis identified 863 metabolites, of which 447 were significantly altered in ozone-exposed rats (adjusted p-value < .1, absolute fold change > 20%). A total of 6 genes were differentially expressed in all 6 tissues. Glucocorticoid signaling, hypoxia, and GPCR signaling were commonly changed, but ozone induced tissue-specific changes in oxidative stress, immune processes, and metabolic pathways. Genes upregulated by TNF-mediated NFkB signaling were differentially expressed in all ozone-exposed tissues, but those defining inflammatory response were tissue-specific. Upstream predictor analysis identified common mediators of effects including glucocorticoids, although the specific genes responsible for these predictors varied by tissue. Metabolomic analysis showed major changes in lipids, amino acids, and metabolites linked to the gut microbiome, concordant with transcriptional changes identified through pathway analysis within liver, muscle, and adipose tissues. The distribution of receptors and transcriptional mechanisms underlying the ozone-induced stress response are tissue-specific and involve induction of unique gene networks and metabolic phenotypes, but the shared initiating triggers converge into shared pathway-level responses. This multi-tissue transcriptomic analysis, combined with circulating metabolomic assessment, allows characterization of the systemic inhaled pollutant-induced stress response.