bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS STRUKTURAL SEMIOTIK NASKAH MONOLOG “SUKETI” KARYA KHOLIK SETIAWAN
Authors: Sutardi Edi , Budi Budi Dharma
Published: October 2023

Penelitian ini bertujuan untuk mendeskripsikan (1) unsur-unsur intrinsik dalam monolog dengan judul “SUKETI” karya; Kholik Setiawan (2) keterkaitan antarunsur intrinsik, serta (3) makna yang didapatkan melalui tanda dan acuannya (ikon, indeks, dan simbol).

Sumber data penelitian ini adalah naskah monolog dengan judul Suketi karya Kholik Setiawan. Naskah tersebut ditulis pada tahun 2022. Pendekatan penelitian yang digunakan yaitu pendekatan objektif. Penelitian ini merupakan penelitian deskriptif kualitatif. Data deskriptif diperoleh melalui teknik baca dan catat. Keabsahan data diperoleh melalui validitas semantis dan reliabilitas interrater dan intrarater.

Hasil penelitian adalah sebagai berikut. (1) Unsur intrinsik meliputi alur, latar, penokohan, dan tema. Alur yang digunakan adalah alur dinamis. Latar tempat yang memungkinkan peristiwa terjadi dan mencerminkan suasana hati tokohnya yaitu  ruang tamu rumah dari Suketi dan Darwin. Latar tempat tersebut, termasuk latar Sabionetta, secara tidak langsung menggambarkan watak tokoh dan memperjelas proposisi simbolik para tokohnya. Latar waktu peristiwa naskah sastra monolog ini adalah pada zaman sekarang, abad 21, dalam peristiwa tergambarkan dari jam pagi sampai malam. Tokoh protagonis  dalam cerita tersebut adalah Suketi dan Darwin sebagai tokoh Antagonis, serta tokoh lelaki tanpa nama menduduki tokoh tritagonis. Tema drama ini adalah psikologi menggambarkan kehidupan keluarga suami istri yang sama megalami masalah biologis dan menimbulkan efek trauma besar sehingga tokoh mengalami depresi berat. (2)  Keterkaitan antar unsur intrinsik diikat oleh dialog dan konflik. Konflik tersebut                                muncul dalam alur, latar, dan penokohan. (3) Hubungan antara tanda dan acuannya berupa ikon, indeks, dan simbol. Ketiga tanda tersebut memperjelas makna drama yaitu perlawanan atas situasi hidup tokoh dalam cerita.
PENINGKATAN KEMAMPUAN GURU DALAM MENGGUNAKAN MODEL PEMBELAJARAN DISCOVERY LEARNING MELALUI SUPERVISI AKADEMIK DI SMPN 2 PIANI
Authors: Purwono
Published: October 2023

Penelitian ini berjudul: “Peningkatan Kemampuan Guru Dalam Menggunakan Model Pembelajaran Discovery Learning Melalui Supervisi Di SMPN 2 Piani”. Tujuan penelitian tindakan sekolah ini adalah untuk meningkatkan Kemampuan Guru Dalam Menggunakan Model Pembelajaran Discovery Learning Melalui Supervisi Akademik Di SMPN 2 Piani. Metode yang digunakan pada penelitian ini adalah Penelitian Tindakan (action Research) yang terdiri dari 2 (dua) siklus, dan setiap siklus terdiri dari: Perencanaan, Pelaksanaan, Pengamatan, dan refleksi.

Hasil penelitian menunjukkan bahwa (1) Pada komponen Instrumen Monitoring Supervisi, terlihat peningkatan dari 78% pada Siklus I, menjadi 92% pada siklus II (2) Pada Komponen Instrumen Peningkatan Kompetensi Kepala Sekolah , terdapat peningkatan Kompetensi dari 80,0% pada siklus I menjadi 92% pada siklus II;(3) Dalam Komponen Instrumen Evaluasi Hasil Supervisi, yang didalamnya memuat langkah-langkah model pembelajaran,terlihat adanya peningkatan yang signifikan dari yang semula hanya 74% pada siklus I menjadi 89% pada siklus II (4) Pada komponen Instrumen Peningkatan Prestasi Peserta Didik juga terdapat adanya peningkatan dari 79% pada siklus I, menjadi 94% setelah siklus II; (5) Peningkatan yang cukup signifikan juga dapat kita lihat pada komponen Instrumen Studens Welbeing (Kebahagiaan Siswa) . Dari yang semula hanya 73% pada siklus I dan berhasil mencapai 92% pada akhir siklus 2.

Melihat data perolehan hasil penelitian dalam   kegiatan penelitian tindakan sekolah ini, dapat disimpulkan bahwa penggunaan model pembelajaran discovery learning melalui supervisi akademik  dapat meningkatkan kemampuan guru di SMPN 2 Piani
THE INFLUENCE OF GAMIFICATION ON THE INNOVATIVE MOTIVATION OF EMPLOYEES
Authors: KULESHOV S.M. , TSARYUK D.A.
Published: October 2023

The purpose of this work is to study the influence of gamification on the innovative motivation of employees. The main trends related to labor motivation in the market were studied. In the course of the analysis, promising tools of labor motivation were identified, among which gamification occupies a special place. Gamification acts as a management tool, which consists in integrating game mechanics into the workflow. The structural elements of gamification, its types, properties and stages of application were considered. In addition, the features of the application of gamification in the global context were studied, as well as the features of Russian practice were determined. To determine the impact of gamification on the innovative motivation of employees, an empirical study was conducted aimed at interviewing the heads of organizations and their employees. Conclusions. Gamification acts as a promising direction of labor motivation and is highly appreciated by both managers and employees. At the same time, a number of problems are highlighted that make it difficult to implement the principles of gamification, especially for small enterprises. The expansion of the use of gamification in the management environment has a positive impact on the prospects of this tool, since at the moment it is used in a limited number of areas.
AMERİKAN MEDENİ USÛL HUKUKUNDA TARAF SORGUSU
Authors: IŞIK Burak
Published: October 2023

Bu çalışmada Amerikan medeni usûl hukukunda dava malzemesinin toplanması açısından oldukça önemli olan “discovery” sürecinde taraflarca kullanılan yöntemlerden birisi olan “taraf sorgusu” incelenir. Türk hukukuna ve hatta Kıta Avrupası hukuk sistemlerine bütünüyle yabancı olan bu yöntem, taraf vekillerinin uyuşmazlığın karşı tarafını ve tanıkları henüz duruşma aşamasına geçilmeden bizzat sorgulamasına imkân verir, böylece uyuşmazlığın çözümünde rol oynaması muhtemel vakıalar ve deliller tespit edilir. Her ne kadar eleştiriye açık yönleri bulunsa da davanın aydınlatılması ve dolayısıyla uyuşmazlığın çözülmesi açısından -taraflarca getirilme ilkesiyle uyumlu olarak- etkinlik sağlayan bu yöntem, hukuk sistemleri arasındaki yakınlaşma da dikkate alındığında, incelemeye değerdir. Bu doğrultuda çalışmada, taraf sorgusu tanıtılmaya çalışılır; faaliyetin amacı, kapsamı ve usûlüne ilişkin açıklamalara yer verilir.
Teaching Academic Writing in English at Arab Universities: Considering the Contexts
Authors: Moody James
Published: October 2023

Academic writing has been a contested area of tertiary English-language education throughout the world. At universities in Arabic-speaking countries, where English is studied as a foreign language, there is a tendency to accept pedagogical theories and practices developed elsewhere, particularly in first- and second-language environments. The unique position of academic writing at Arab universities is first examined by focusing on the perspective of two communicative contexts: the speech community (where Arabic is the main language) and the discourse community (in which the novice student is supposed to be “conversing” with international scholars). Anomalies concerning misconceptions about these contexts and the relations between them are then considered in regard to three areas of conflict: between reading and writing, Arabic and English, and skills and content. On the basis of experience and examples, mainly from Qatar and Oman, it is argued that closer integration is required in each case, which involves changes in teaching programs and administrative structures. Bringing together areas of study that have tended to remain separate can result in what has been termed a “pedagogy of possibility”, one that makes the teaching of academic writing more responsive to the needs of both speech and discourse communities. .
Reflections on the Experience of Using E-learning in English Language and Literature Teaching at Al-Quds Open University
Authors: Abbas Insaf
Published: October 2023

Education in "the Information Age" is changing rapidly. Recent developments in technology have opened new vistas for online education, which offers students the flexibility to learn at convenient times and places. This study focuses on illustrating the importance of modern technology in education especially distance education. It displays the experience of utilizing new forms of technology in online education to enhance learning in general and English language teaching in particular. It further highlights some experiments that the researcher has been implementing in e-learning. The study uses the descriptive analytical approach as these experiments are described and evaluated and recommendations given..
Learning outcomes: A Case Study from Arab Open University
Authors: Abdullah Al-Ghamdi Ghurmallah
Published: October 2023

This paper is organized as follows: the first part gives an overview of the AOU, the concept of Open Learning and the structure of the Faculty of Language Studies (FLS). The second part addresses the issue of the learning outcomes in some details highlighting the concept, advantages of learning outcomes and the relationship between learning outcomes and methods of assessment as employed at the AOU. The third part addresses the field work, presented in a form of questionnaire to be given to students. Results are then tabulated, followed by discussion. The final part addresses the implication and recommendation of the study..
Politisasi Birokrasi Oleh Calon Pemimpin Daerah Dalam Pemilihan Kepala Daerah; Kasus Pilkada di Kabupaten Lebong Tahun 2015
Authors: Al islam Muhammad sheif , Wati Ira , Rusta Andri , Fajri Mhd
Published: October 2023

kasus yang memiliki kaitan dengan politisasi birokrasi yang dilakukan oleh salah satu petahana Bupati, berdomisili di Kabupaten Lebong Provinsi Bengkulu yang terjadi pada pra Pilkada tahun 2015. Dalam kasus tersebut, aktor yang terlibat merupakan petahana dari Bupati Lebong dan birokrat Kepala Bidang Kebersihan, Hadian Tarzon, di Badan Lingkungan Hidup, Kebersihan dan Pertamanan (BLHKP) Kab. Lebong. Aktor dari kasus ini melancarkan aksi politik uang yang diberikan kepada sejumlah pekerja ketika melakukan kampanye di kantor BLHPK, yaitu oleh Hadian Tarzon selaku kabid kebersihan. Hadian Tarzon yang merupakan seorang birokrat dan Aparat Sipil Negara (ASN) seharusnya tidak diperkenankan untuk melakukan kampanye dan menyatakan keberpihakannya atas paslon yang mengikuti Pilkada pada saat itu karena hal tersebut melanggar kode etik sebagai ASN yang harus bersifat netral atau tidak mendukung pasangan calon manapun dan ataupun menjadi tim sukses. Terlebih tindakan yang dilakukannya ialah memberikan sejumlah uang kepada para pekerja kantor, dimana hal tersebut sudah jelas bertentangan dengan undang-undang No. 5 tahun 2014, menyebutkan bahwa setiap pegawai ASN tidak berpihak dari segala bentuk pengaruh mana pun dan tidak memihak kepada kepentingan siapapun. Penelitian ini menggunakan metode kualitatif dengan jenis pengumpulan data  studi literatur. Ketimpangan terjadi ketika Hadian Tarzon kembali mendapatkan jabatannya setelah paslon yang didukungnya kembali menang, dan terdapat ketimpangan hukum disana sebab dapat terulang kembali sampai kapanpun meletakkan harapan kemenangan dengan kemungkinan  50:50Kata kunci; Politisasi Birokrasi; Pilkada; Kampanye; Ketimpangan Hukum.
STRATEGI AKOMODASI KOMUNIKASI ANTAR-BUDAYA KARYAWAN ETNIS JAWA-BETAWI DI LINGKUNGAN SUSHI TEI SUDIRMAN
Authors: Andini Shiva Trie , Fajarina Fajarina , Siregar Ballian
Published: October 2023

Fenomena interaksi keragaman budaya dalam suatu kelompok kerja menunjukkan adanya konvergensi, divergensi, dan akomodasi yang berlebihan di lingkungan kerja Restoran Sushi Tei Citywalk Sudirman, Jakarta. Tujuan dari penelitian ini adalah untuk mendeskripsikan bentuk-bentuk strategi akomodasi karyawan etnis Jawa dan Betawi serta memahami hambatan interaksi komunikasi antar budaya. Penelitian ini menggunakan metode kualitatif dengan pendekatan etnografi. Hasil penelitian menunjukkan bentuk strategi akomodasi komunikasi karyawan etnis Jawa dan Betawi adalah konvergensi, divergensi, dan akomodasi berlebihan. Kedua etnis ini memiliki landasan budaya yang sangat berbeda, sehingga menjadi faktor yang mendorong dan juga menghambat akomodasi komunikasi antar budaya. Hambatan yang dihadapi oleh kedua etnis ini sama, yaitu perbedaan bahasa dan gaya bicara yang kontras.

Kata Kunci: intercultural-communication, Javanese, Betawi, Sushi Tei
Circular Economy Initiatives in Supply Chain: A Systematic Literature Review and Future Research Directions
Authors: Chokshi Hemakshi , Agrawal Rohit , Samadhiya Ashutosh , Kumar Anil
Published: October 2023

The concept of a circular economy (CE) entails developing a closed-loop system that strives to reduce waste, which adversely impacts the environment, while also minimising the use of resources (both natural and technical). The authors perform a thorough literature assessment of relevant papers using bibliometric and network analysis methodologies to identify the major components involved in a CE and solve the issues associated with implementing CE practices in the supply chain (SC). One of the objectives of this study is to determine current trends in SC based on the CE from 2013 to 2022. 101 articles are selected for in-depth analysis based on a bibliometric and network analysis approach. The review sheds light on the most important success factors of CE practices in SC across a variety of industries, as well as current and upcoming research trends. This review identifies research gaps and highlights additional theoretical approaches to the critical success factors of CE practices in the SC. The findings of this research will enable organisations to better understand the challenges and opportunities associated with CE practices and develop more efficient and sustainable SC strategies.
Transaction Processing Policies in a Flexible Shuttle-based Storage and Re-trieval System by Real-time Data Tracking under Agent-based Modelling
Authors: Y. Ekren Banu
Published: October 2023

This study investigates priority assignment rules (PARs) for transaction processing in automated warehouses featuring a shuttle-based storage and retrieval system (SBSRS). By incorporating real-time data tracking through agent-based modeling, the research explores the unique aspect of the SBSRS design, which involves flexible travel of robotic order picker shuttles be-tween tiers. The paper proposes PARs under agent-based modeling to enhance multi-objective performance metrics, including average flow time (AFT), maximum flow time (MFT), outlier transaction AFT, and standard deviations of flow times (SD) within the system. Experimental evaluations are conducted with various warehouse designs, comparing the results against commonly used static scheduling rules. The findings demonstrate that real-time tracking policies significantly improve system performance. Specifically, prioritizing the processing of outliers based on transaction waiting time enhances MFT, SD, and other performance metrics, while minimizing adverse effects on AFT. Certain rules exhibit notable improvements in MFT and SD, while others achieve the lowest AFT values among all experiments. This paper contributes to the existing literature by presenting a multi-objective performance improvement procedure and highlighting the advantages of real-time data track-ing-based scheduling policies in automated warehousing systems.
Numerical Investigation of Thermoelastic Crack Interactions in Various Materials using a Novel Enrichment Approach
Authors: Awasthi Ayush , Pant Mohit
Published: October 2023

Crack interaction studies play a crucial role in understanding and predicting the fracture behaviour of various engineering components subjected to thermomechanical loads. The present work investigates the interaction effect of multiple cracks in different types of material subjected to thermoelastic loadings using Element free Galerkin method (EFGM). These materials include isotropic material, orthotropic material, functionally graded material, and layered material. These all materials are subjected to thermoelastic loads in presence of multiple cracks to investigate the effect of crack interactions. A novel modified Intrinsic enrichment has been proposed to precisely capture the interaction effect and stress fields in the presence of multiple cracks. The proposed algorithm has been tested for a benchmark problem and it produced better stress fields in comparison to the conventional EFGM procedure. Stress intensity factors corresponding to variations in crack parameters have been evaluated concerning with the primary crack. Results reveal that presence of multiple cracks alters the crack tip stress fields owing to the interaction effect i.e shielding or amplification. Additionally, parameters such as crack length, crack orientation, distance between cracks, and domain properties greatly influence the stress intensity factor of the primary crack. These parameters exhibit varying behaviour under distinct circumstances, and their effects have been thoroughly analysed. Current work provides valuable insights into the effects of crack interactions in different media under thermoelastic loadings, thereby ensuring the structural integrity and durability of these materials for practical applications.
Explore the changes of intestinal flora in patients with coronavirus disease 2019 based on bioinformatics
Authors: Huang Gangding , Mao Yanning , Zhang Weiwei , Luo Qi , Xie Rong , Huang Dongmei , Liang Yumei
Published: October 2023

Background

Studies have revealed that there were significant changes in intestinal flora composition in patients with coronavirus disease 2019 (COVID-19) compared to non-COVID-19 patients, regardless of whether they were treated with medication. Therefore, a comprehensive study of the intestinal flora of COVID-19 patients is needed to further understand the mechanisms of COVID-19 development.

Methods

In total, 20 healthy samples and 20 COVID-19 samples were collected in this study. Firstly, alpha diversity and beta diversity were analyzed to assess whether there were difference in species richness and diversity as well as species composition between COVID-19 and control groups. The observed features index, Evenness index, PD index, and Shannon index were utilized to measure alpha diversity. The principal coordinates analysis (PCoA) and non-metric multidimensional scaling (NMDS) were performed to analyzed beta diversity. Linear discriminant analysis Effect Size (LEfSe) was utilized to analyze the variability in the abundance of bacterial taxa from different classification levels. The random forest (RF), Least absolute shrinkage and selection operator (LASSO), and univariate logistic regression were utilized to identify key Amplicon Sequence Variant (ASVs). Finally, the relevant networks of bacterial taxa were created in COVID-19 and control groups, separately.

Results

There were more species in the control group than in COVID-19 group. The observed features index, Shannon index, and Evenness index in the control groups were markedly higher than in the COVID-19 group. Therefore, there were marked variations in bacterial taxa composition between the COVID-19 and control groups. The nine bacterial taxa were significantly more abundant in the COVID-19 group, such as g-Streptococcus, f-Streptococcaceae, o-Lactobacillales, c-Bacilli and so on. In the control group, 26 bacterial taxa were significantly more abundant, such as c-Clostrjdia, o-Oscillospirales, f-Ruminococcaceae, etc. The 5 key ASVs were obtained through taking the intersection of the characteristic ASVs obtained by the three algorithms, namely ASV6, ASV53, ASV92, ASV96, and ASV105, which had diagnostic value for COVID-19. The relevance network in the control group was more complex compared to the COVID-19 group.

Conclusion

Our findings provide five key ASVs for diagnosis of COVID-19, providing a scientific reference for further studies of COVID-19.
Near-field variability of evaposublimation in a montane conifer forest
Authors: Drake Stephen A. , Nolin Anne W. , Oldroyd Holly J.
Published: October 2023

Methods that combine in-situ measurements, statistical methods, and model simulations with remotely sensed data provide a pathway for improving the robustness of surface flux products. For this research, we acquired eddy-covariance fluxes along a five-tower transect in a snowy montane forest over three consecutive winters to characterize near-field variability of the subcanopy environment. The novel experiment design enabled discriminating near-field evaposublimation sources. Boosted regression trees reveal that the predictive capacity of state variables change with season and storm cycle frequency. High rates of post-storm evaposublimation of canopy-intercepted snow at this site were constrained by short residence time of snow in the canopy due to throughfall and melt. The snow melt-out date for open vs. closed canopy conditions depended on total snowfall accumulation. Compared with low accumulation years, the snow melt-out date under the dense canopy during the high accumulation winter was later than for the open area, as shading became more important later in the season. The field experiments informed an environmental response function that was used to integrate ERA5-Land latent heat flux data at 20-km nominal resolution with USFS Tree Canopy Fraction data at 30-m resolution and showed near-field flux variability that was not resolved in model simulations. Previous evaposublimation results from experiments in alpine and subalpine environments do not directly translate to a montane forest due to differences in process rates.
ChatGPT, a brand-new tool to strengthen timeless competencies
Authors: Michalon Barthélémy , Camacho-Zuñiga Claudia
Published: October 2023

In November 2022, the public release of ChatGPT, an artificial intelligence (AI)-based natural language model, was a groundbreaking point in many sectors of human life and education was not the exception. We describe how ChatGPT was integrated in an undergraduate course for an International Relations program in a private Mexican university. Under an action research methodology, we introduced this novel instrument in a course on Future Studies. Students were evaluated on their ability to explain to ChatGPT several discipline-specific methods and to make the AI implement these methods step by step. After six such activities, the outcomes evidenced that the students not only learned how to use the new AI tool and deepen their understanding of prospective methods, but also strengthened three soft or transversal competencies: communication, critical thinking, and logical and methodical reasoning. These results are promising in the framework of Skills for Industry 4.0 and Education for Sustainable Development; even more, they demonstrate how ChatGPT created an opportunity for the students to strengthen, and for the professor to assess, time-tested competencies. This is a call-to action for faculty and educational institutions to incorporate AI in their instructional design, not only to prepare our graduates for professional environments where they will collaborate with these technologies but also to enhance the quality and relevance of higher education in the digital age. Therefore, this work contributes to the growing body of research on how Artificial Intelligence (AI) can be used in higher education settings to enhance learning experiences and outcomes.
Treatment with the dual-incretin agonist DA-CH5 demonstrates potent therapeutic effect in a rat model of Wolfram Syndrome
Authors: Jagomäe Toomas , Gaur Nayana , Seppa Kadri , Reimets Riin , Pastak Marko , Plaas Mihkel , Kaasik Allen , Vasar Eero , Plaas Mario
Published: October 2023

Aim

Wolfram Syndrome (WS) is a rare condition caused by mutations in Wfs1, with a poor prognosis and no cure. Mono-agonists targeting the incretin glucagon-like-peptide 1 (GLP-1) have demonstrated disease-modifying potential in pre-clinical and clinical settings. Dual agonists that target GLP-1 and glucose-dependent insulinotropic polypeptide (GIP-1) are reportedly more efficacious; hence, we evaluated the therapeutic potential of dual incretin agonism in a loss-of-function rat model of WS.

Methods

Eight-month-old Wfs1 knock-out (KO) and wild-type control rats were continuously treated with either the dual agonist DA-CH5 or saline for four months. Glycemic profile, visual acuity and hearing sensitivity were longitudinally monitored pre-treatment, and then at 10.5 and 12 months. Pancreata and retina were harvested for immunohistological analysis.

Results

DA-CH5 therapy reversed glucose intolerance in KO rats and provided lasting anti-diabetogenic protection. Treatment also reversed intra-islet alterations, including reduced endocrine islet area and β-cell density, indicating its regenerative potential. Although no rescue effect was noted for hearing loss, visual acuity and retinal ganglion cell density were better preserved in DA-CH5-treated rats.

Conclusion

We present preclinical evidence for the pleiotropic therapeutic effects of long-term dual incretin agonist treatment; effects were seen despite treatment beginning after symptom-onset, indicating reversal of disease progression. Dual incretins represent a promising therapeutic avenue for WS patients.
The role of the tumor microenvironment in endocrine therapy resistance in hormone receptor-positive breast cancer
Authors: Yuan Jie , Yang Li , Li Zhi , Zhang Hua , Wang Qun , Huang Jun , Wang Bei , Mohan Chakrabhavi Dhananjaya , Sethi Gautam , Wang Geng
Published: October 2023

Endocrine therapy is the prominent strategy for the treatment of hormone-positive breast cancers. The emergence of resistance to endocrine therapy is a major health concern among hormone-positive breast cancer patients. Resistance to endocrine therapy demands the design of newer therapeutic strategies. The understanding of underlying molecular mechanisms of endocrine resistance, components of the tumor microenvironment (TME), and interaction of resistant breast cancer cells with the cellular/acellular components of the intratumoral environment are essential to formulate new therapeutic strategies for the treatment of endocrine therapy-resistant breast cancers. In the first half of the article, we have discussed the general mechanisms (including mutations in estrogen receptor gene, reregulated activation of signaling pathways, epigenetic changes, and cell cycle alteration) responsible for endocrine therapy resistance in hormone-positive breast cancers. In the latter half, we have emphasized the precise role of cellular (cancer-associated fibroblasts, immune cells, and cancer stem cells) and acellular components (collagen, fibronectin, and laminin) of TME in the development of endocrine resistance in hormone-positive breast cancers. In sum, the article provides an overview of the relationship between endocrine resistance and TME in hormone-positive breast cancers.
Study on debugging of lightning impulse voltage waveform for large capacity ±800kV converter transformer in high altitude area
Authors: Jiang Ling , Wang Shengfu , Kang Jun , Yang Xiaoku , Yao Xiuyuan , Zhang Yitao , Bao Zhenghong , Zhang Yongsheng
Published: October 2023

The Qinghai-Henan ±800kV UHV DC power transmission project is an UHV channel specially constructed for clean energy delivery, ±800kV converter transformers of the project are assembled and tested in Xining at an altitude of 2500 m, full-wave lightning impulse test is one of the main means to evaluate converter transformer insulation performance, its valve side lightning impulse voltage waveform parameters often fail to meet IEC60076-3 standards because of the large winding to the ground capacitance. In order to effectively assess the lightning tolerance of converter transformer, a simulation model of impulse test circuit of ±800kV converter transformer was built based on the existing 6000kV/560kJ impulse voltage generator and ATP-EMTP simulation software, three kinds of optimization methods are proposed, which include increasing the wavetail resistance, increasing load capacitor, and connecting the wave head in parallel with different inductors, the results show that the single optimization method can not meet the waveform requirements. Therefore, a combinatorial optimization test method of increasing the wavetail resistance and connecting the wave head in parallel with different inductors is proposed, which is completed and verified in the full-wave lightning impulse test on the valve side of Qinghai ±800kV high-end converter transformer, the results show that the optimized test waveform parameters meet the standard requirements.
Comprehensive assessment of building energy consumption in hot summer and cold winter areas based on carbon tax considerations
Authors: Xie Jing , Miao Xinyue , Dewancker Bart Julien , Xu Tongyu , Zhao Xueyuan , Shi Chunyan , Wei Xindong
Published: October 2023

With the rapid development of economy and urbanization in China, cities are expanding rapidly and more constructions are being built, then lead to the fast-growing part in urban residential energy consumption. In China’s hot summer and cold winter regions, many buildings are not centrally heated and commonly rely on electrical equipment such as air conditioners which are all energy-inefficient thermoregulation devices. In order to analyze the relationship between building energy consumption and the energy efficiency ratio (EER) of air conditioning and the area of photovoltaic (PV) on the roof, the influence of the building envelope on building energy consumption in hot summer and cold winter regions in China is clarified. This paper uses energy plus software to analyze the impact of different EER and PV area on building energy consumption using a typical case study of a public health upgrading project in the eastern part of the Wuxing district, Huzhou, Zhejiang province in China. The simulation results show that the factor that has a greater impact on the energy consumption of the health center in summer is the PV area parameter of the building compared to the performance parameters of the air conditioning equipment. The PV area parameter settings and air conditioning equipment performance adjustments are dependent on the actual situation and the comprehensive energy saving rate of the outpatient building for public health in the eastern part of Tai Wu Xing District can exceed 50%. Finally, an economic analysis of the carbon tax and input costs is carried out, and the best combination is of which 60% of the area covered by Longi Green PV panels and an air conditioning efficiency ratio of 4.87. The research result combines renewable energy and efficient equipment to achieve dual optimization of environmental and economic aspects of building energy consumption, while providing reference opinions on the comprehensive evaluation method of building energy consumption from the perspective of carbon tax.
Evaluating methods for determining mercury concentrations in ancient marine fish and mammal bones as an approach to assessing millennial-scale fluctuations in marine ecosystems
Authors: Avery Julie P. , Castellini J. Margaret , Misarti Nicole , Keenan Mary , Gastaldi Angela , Funk Caroline , O’Hara Todd M. , Rea Lorrie D.
Published: October 2023

Millennial-scale datasets of heavy metals in biota are difficult to obtain but are important for determining patterns and underlying drivers of toxicant concentrations. This is particularly important to better discriminate contemporary natural and anthropogenic sources. Globally mercury is a contaminant of concern. Post-industrial increases in mercury in arctic biota have been documented and monitoring of Steller sea lions (Eumetopias jubatus) in the Aleutian Islands, Alaska, has revealed a high proportion of pups with fur mercury concentrations above thresholds of concern in some regions. As bone is a tissue that is well preserved in archeological middens, it may prove useful for developing long-term mercury data sets under appropriate conditions. The goal of this study was to evaluate methodologies for measuring mercury concentration in Steller sea lion bone using a direct mercury analyzer, considering sample preparation methods and variability among bone tissue types (e.g., compact versus spongy bone). Finally, we directly compare sensitivity and precision of two different direct mercury analyzer models. Based on the methods presented here, direct mercury analysis using the Nippon MA-3000 can quantify small (ppb) quantities of Hg accurately and precisely in 20 to 60mg of bone with minimal specimen processing. The described method is efficient, relatively inexpensive, and requires minimal bone, conserving rare and valuable specimens. Hydrogen peroxide cleaning and collagen extraction were not required, and may be detrimental for optimal Hg quantification in bone. Further, while homogenization of distinct compact and spongy bone did not impact concentration determination, variance of technical replicates was lower improving quantitation precision. Most importantly, significant differences between compact and spongy bone exist within some individual specimen; however, the difference is not consistent and may indicate differential Hg exposure windows influenced by turnover rate of bone types. We conclude bone provides a natural archive for mercury ecosystem dynamics over millennial time scales in regions where appropriate samples are available. Compact bone has lower and less variable [THg] simplifying analysis and interpretation of data; however, the more dynamic concentrations observed in spongy bone should not be dismissed as invaluable due to their variability in [THg]. Comparisons of [THg] between bone type within individual may provide insight into more acute changes in mercury exposure within an individual’s lifetime.
Offshore wind power policies and green total factor productivity: empirical evidence from coastal China
Authors: Zhang Junjie , Wei Zhuxia
Published: October 2023

Introduction

Green and high-quality development requires the transformation and upgrading the energy structure. As a clean and efficient new energy, the development of offshore wind power is related to the achievement of green development and the realization of the dual carbon goals.

Methods

Based on the perspective of green total factor production, this study aims to explore the impact of offshore wind power policies (OWPPs) on green and high-quality development. Taking 11 coastal areas of China from 2004 to 2020 as samples, this paper empirically tested the impact of OWPPs on green total factor productivity (GTFP) by using propensity score matching difference-in-differences method (PSM-DID).

Results and discussion

The results show that OWPPs have a significant positive impact on GTFP. The robustness test further verifies the results, and the provincial difference is significant. By stimulating technological innovation and reducing energy intensity, OWPPs have improved GTFP, but increasing marketization level is a long way off.
Isoform switching leads to downregulation of cytokine producing genes in estrogen receptor positive breast cancer
Authors: Khan Mohammad Shahbaz , Hanif Waqar , Alsakhen Nada , Jabbar Basit , Shamkh Israa M. , Alsaiari Ahad Amer , Almehmadi Mazen , Alghamdi Saad , Shakoori Afnan , Al Farraj Dunia A. , Almutairi Saeedah Musaed , Hussein Issa Mohammed Yasser , Abouzied Amr S. , Rehman Aziz-Ur , Huwaimel Bader
Published: October 2023

Objective: Estrogen receptor breast cancer (BC) is characterized by the expression of estrogen receptors. It is the most common cancer among women, with an incidence rate of 2.26 million cases worldwide. The aim of this study was to identify differentially expressed genes and isoform switching between estrogen receptor positive and triple negative BC samples.

Methods: The data were collected from ArrayExpress, followed by preprocessing and subsequent mapping from HISAT2. Read quantification was performed by StringTie, and then R package ballgown was used to perform differential expression analysis. Functional enrichment analysis was conducted using Enrichr, and then immune genes were shortlisted based on the ScType marker database. Isoform switch analysis was also performed using the IsoformSwitchAnalyzeR package.

Results: A total of 9,771 differentially expressed genes were identified, of which 86 were upregulated and 117 were downregulated. Six genes were identified as mainly associated with estrogen receptor positive BC, while a novel set of ten genes were found which have not previously been reported in estrogen receptor positive BC. Furthermore, alternative splicing and subsequent isoform usage in the immune system related genes were determined.

Conclusion: This study identified the differential usage of isoforms in the immune system related genes in cancer cells that suggest immunosuppression due to the dysregulation of CXCR chemokine receptor binding, iron ion binding, and cytokine activity.
Machine learning-based identification of CYBB and FCAR as potential neutrophil extracellular trap-related treatment targets in sepsis
Authors: You GuoHua , Zhao XueGang , Liu JianRong , Yao Kang , Yi XiaoMeng , Chen HaiTian , Wei XuXia , Huang YiNong , Yang XingYe , Lei YunGuo , Lin ZhiPeng , He YuFeng , Fan MingMing , An YuLing , Lu TongYu , Lv HaiJin , Sui Xin , Yi HuiMin
Published: October 2023

Objective

Sepsis related injury has gradually become the main cause of death in non-cardiac patients in intensive care units, but the underlying pathological and physiological mechanisms remain unclear. The Third International Consensus Definitions for Sepsis and Septic Shock (SEPSIS-3) definition emphasized organ dysfunction caused by infection. Neutrophil extracellular traps (NETs) can cause inflammation and have key roles in sepsis organ failure; however, the role of NETs-related genes in sepsis is unknown. Here, we sought to identify key NETs-related genes associate with sepsis.

Methods

Datasets GSE65682 and GSE145227, including data from 770 patients with sepsis and 54 healthy controls, were downloaded from the GEO database and split into training and validation sets. Differentially expressed genes (DEGs) were identified and weighted gene co-expression network analysis (WGCNA) performed. A machine learning approach was applied to identify key genes, which were used to construct functional networks. Key genes associated with diagnosis and survival of sepsis were screened out. Finally, mouse and human blood samples were collected for RT-qPCR verification and flow cytometry analysis. Multiple organs injury, apoptosis and NETs expression were measured to evaluated effects of sulforaphane (SFN).

Results

Analysis of the obtained DEGs and WGCNA screened a total of 3396 genes in 3 modules, and intersection of the results of both analyses with 69 NETs-related genes, screened out seven genes (S100A12, SLC22A4, FCAR, CYBB, PADI4, DNASE1, MMP9) using machine learning algorithms. Of these, CYBB and FCAR were independent predictors of poor survival in patients with sepsis. Administration of SFN significantly alleviated murine lung NETs expression and injury, accompanied by whole blood CYBB mRNA level.

Conclusion

CYBB and FCAR may be reliable biomarkers of survival in patients with sepsis, as well as potential targets for sepsis treatment. SFN significantly alleviated NETs-related organs injury, suggesting the therapeutic potential by targeting CYBB in the future.
Effect of sodium bicarbonate infusion on hospital mortality in acute kidney injury patients with metabolic acidosis
Authors: Wang Yunting , Chen Ling , Guo Guangfeng , Gao Youyuan , Gan Hua
Published: October 2023

Background

Physicians usually consider that sodium bicarbonate (SB) infusion can be used for metabolic acidosis; however, there is little evidence available to assess its effect on hospital mortality in large AKI cohorts. Here, we investigated the effect of SB infusion in patients with AKI complicated by metabolic acidosis.

Method

Patients with AKI complicated by metabolic acidosis were screened from the MIMIC-IV database. A propensity score analysis (PSA) was used to decrease baseline differences in the probability of receiving SB. The marginal structural Cox model (MSCM) was employed to adjust for both baseline and time-varying confounding factors.

Results

A total of 1853 patients with AKI complicated with metabolic acidosis were included in our study. A total of 390 pairs of patients were divided into an SB infusion group and a non-SB infusion group. The SB infusion group had more serious and worse laboratory indicators, including lower pH [7.19 (0.11) vs. 7.26 (0.07)] and bicarbonate concentration (BC) [12.36 (4.26) vs. 15.96 (3.25) mmol/l]. While there was no significant effect on overall hospital mortality in AKI patients complicated with metabolic acidosis (p = 0.056), SB infusion was observed to have beneficial correlation on hospital mortality in patients with high AG acidosis (AG > 18 mmol/L) (p = 0.012). Similar results were replicated with the MSCM.

Conclusion

We found that SB infusion in AKI patients with metabolic acidosis is not beneficial for hospital mortality. However, SB infusion for AKI patients and high AG metabolic acidosis significantly improved hospital mortality. Further larger randomized controlled trials are needed to confirm these results.
Isolation, identification, and pathogenicity of porcine epidemic diarrhea virus
Authors: Sun Yingshuo , Gong Ting , Wu Dongdong , Feng Yongzhi , Gao Qi , Xing Jiabao , Zheng Xiaoyu , Song Zebu , Liu Xing , Chen Xiongnan , Sun Yankuo , Zhang Guihong , Gong Lang
Published: October 2023

Porcine epidemic diarrhea (PED) is an enterophilic infectious disease caused by the porcine epidemic diarrhea virus (PEDV), which can lead to dehydration-like diarrhea in piglets with a mortality rate of up to 100%, causing huge economic losses to the global pig industry. In this study, we isolated two PEDV strains, FS202201 and JY202201, from diarrheal samples collected from two new PED outbreak farms in 2022. We performed phylogenetic analysis of the S gene and whole gene sequence. The effects of the different mutations on viral pathogenicity were investigated using piglet challenge experiments. The results showed that both strains belong to the G2c subtype, a widely prevalent virulent strain. Compared with FS202201, JY202201 harbored substitution and deletion mutations in nsp1. Both FS202201 and JY202201 infected piglets showed severe diarrhea and significant intestinal tissue lesions at an infection dose of 104 TCID50/mL, with a mortality rate of 50%; however, JY202201 required an additional day to reach mortality stabilization. An infection dose of 103 TCID50/mL reduced diarrhea and intestinal tissue lesions in piglets, with mortality rates of the two strains at 16.7% and 0%, respectively. In addition, PEDV was detected in the heart, liver, spleen, lungs, kidneys, mesenteric lymph nodes, stomach, large intestine, duodenum, jejunum, and ileum, with the highest levels in the intestinal tissues. In conclusion, this study enriches the epidemiology of PEDV and provides a theoretical basis for the study of its pathogenic mechanism and prevention through virus isolation, identification, and pathogenicity research on newly identified PED in the main transmission hub area of PEDV in China (Guangdong).
Verbal memory depends on structural hippocampal subfield volume
Authors: Tsalouchidou Panagiota-Eleni , Müller Christina-Julia , Belke Marcus , Zahnert Felix , Menzler Katja , Trinka Eugen , Knake Susanne , Thomschewski Aljoscha
Published: October 2023

Objective

To investigate correlates in hippocampal subfield volume and verbal and visual memory function in patients with temporal lobe epilepsy (TLE), mild amnestic cognitive impairment (MCI) and heathy participants (HP).

Methods

50 right-handed participants were included in this study; 11 patients with temporal lobe epilepsy (TLE), 18 patients with mild amnestic cognitive impairment (MCI) and 21 healthy participants (HP). Verbal memory performance was evaluated via the verbal memory test (VLMT) and visual memory performance via the diagnosticum for cerebral damage (DCM). Hippocampal subfield volumes of T1-weighted Magnetic Resonance Imaging (MRI) scans were computed with FreeSurfer version 7.1. Stepwise correlation analyses were performed between the left hippocampal subfield volumes and learning, free recall, consolidation and recognition performance scores of the VLMT as well as between right hippocampal subfield volumes and visual memory performance.

Results

The volume of the left subicular complex was highly correlated to learning performance (β = 0.284; p = 0.042) and free recall performance in the VLMT (β = 0.434; p = 0.001). The volume of the left CA3 subfield showed a significant correlation to the consolidation performance in the VLMT (β = 0.378; p = 0.006) and recognition performance in the VLMT (β = 0.290; p = 0.037). There was no significant correlation identified between the right hippocampal subfields and the visual memory performance.

Conclusion

The results of this study show verbal memory correlates with hippocampal subfields and support the role of left subiculum and left CA2/CA3 in verbal memory performance.
Bowel dysfunctions after acquired brain injury: a scoping review
Authors: Zandalasini Matteo , Pelizzari Laura , Ciardi Gianluca , Giraudo Donatella , Guasconi Massimo , Paravati Stefano , Lamberti Gianfranco , Frizziero Antonio
Published: October 2023

Bowel dysfunction is a common consequence of neurological diseases and has a major impact on the dignity and quality of life of patients. Evidence on neurogenic bowel is focused on spinal cord injury and multiple sclerosis; few studies have focused on patients with acquired brain injury (ABI). Neurogenic bowel dysfunction is related to a lifelong condition derived from central neurological disease, which further increases disability and social deprivation. The manifestations of neurogenic bowel dysfunction include fecal incontinence and constipation. Almost two out of three patients with central nervous system disorder have bowel impairment. This scoping review aims to comprehend the extent and type of evidence on bowel dysfunction after ABI and present conservative treatment. For this scoping review, the PCC (population, concept, and context) framework was used: patients with ABI and bowel dysfunction; evaluation and treatment; and intensive/extensive rehabilitation path. Ten full-text articles were included in the review. Oral laxatives are the most common treatment. The Functional Independence Measure (FIM) subscale is the most common scale used to assess neurogenic bowel disease (60%), followed by the Rome II and III criteria, and the colon transit time is used to test for constipation; however, no instrumental methods have been used for incontinence. An overlapping between incontinence and constipation, SCI and ABI increase difficulties to manage NBD. The need for a consensus between the rehabilitative and gastroenterological societies on the diagnosis and medical care of NBD.

Systematic review registration Open Science Framework on August 16, 2022 https://doi.org/10.17605/OSF.IO/NEQMA.