bluuebunny/crossref_metadata_2025 huggingface dataset:
Multilineage Dysplasia as Assessed by Immunophenotype in Acute Myeloid Leukemia: A Prognostic Tool in a Genetically Undefined Category
Authors: Mannelli Francesco , Bencini Sara , Piccini Matteo , Gianfaldoni Giacomo , Bonetti Maria Ida , Peruzzi Benedetta , Caporale Roberto , Scappini Barbara , Pancani Fabiana , Ponziani Vanessa , Signori Leonardo , Zizza Michela , Annunziato Francesco , Bosi Alberto
Published: October 2020

Acute myeloid leukemia (AML) “with myelodysplasia-related changes (MRC)” is considered a separate entity by the World Health Organization (WHO) classification of myeloid neoplasms. While anamnestic and cytogenetic criteria provide objective attribution to this subset, with clear unfavorable prognostic significance, the actual role of multi-lineage dysplasia (MLD) as assessed by morphology is debated. The aim of our work was to study MLD by a technique alternative to morphology, which is multiparameter flow cytometry (MFC), in a large series of 302 AML patients intensively treated at our Center. The correlation with morphology we observed in the unselected analysis reiterated the capability of the MFC-based approach at highlighting dysplasia. MLD data, estimated through an immune-phenotypic score (IPS), provided no insight into prognosis when considered overall nor within well-defined genetic categories. Of interest, IPS-related dysplasia conveyed significant prognostic information when we focused on genetically undefined patients, triple-negative for NPM1, FLT3 and CEBPA (TN-AML). In this context, the lack of dysplastic features (IPS_0) correlated with a significantly higher CR rate and longer survival compared to patients showing dysplasia in one or both (neutrophil and erythroid) cell lineages. The impact of IPS category maintained its validity after censoring at allogeneic HSCT and in a multivariate analysis including baseline and treatment-related covariates. In a subgroup featured by the lack of genetic determinants, our data could help address the relative unmet needs in terms of risk assessment and treatment strategy, and provide insight into prediction of response in the rapidly evolving therapeutic scenario of AML.
ROS Overproduction Sensitises Myeloma Cells to Bortezomib-Induced Apoptosis and Alleviates Tumour Microenvironment-Mediated Cell Resistance
Authors: Caillot Mélody , Zylbersztejn Florence , Maitre Elsa , Bourgeais Jérôme , Hérault Olivier , Sola Brigitte
Published: October 2020

Multiple myeloma (MM) is a plasma cell neoplasm that remains incurable due to innate or acquired resistance. Although MM cells produce high intracellular levels of reactive oxygen species (ROS), we hypothesised that they could remain sensitive to ROS unbalance. We tested if the inhibition of ROS, on one hand, or the overproduction of ROS, on the other, could (re)sensitise cells to bortezomib (BTZ). Two drugs were used in a panel of MM cell lines with various responses to BTZ: VAS3947 (VAS), an inhibitor of NADPH oxidase and auranofin (AUR), an inhibitor of thioredoxin reductase (TXNRD1), an antioxidant enzyme overexpressed in MM cells. We used several culture models: in suspension, on a fibronectin layer, in coculture with HS-5 mesenchymal cells, and/or in 3-D culture (or spheroids) to study the response of MM primary cells and cell lines. Several MM cell lines were sensitive to VAS but the combination with BTZ showed antagonistic or additive effects at best. By contrast, in all culture systems studied, the combined AUR/BTZ treatment showed synergistic effects on cell lines, including those less sensitive to BTZ and primary cells. MM cell death is due to the activation of apoptosis and autophagy. Modulating the redox balance of MM cells could be an effective therapy for refractory or relapse post-BTZ patients.
COVID-19-Associated Neurological Disorders: The Potential Route of CNS Invasion and Blood-Brain Barrier Relevance
Authors: Achar Aneesha , Ghosh Chaitali
Published: October 2020

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel human coronavirus that has sparked a global pandemic of the coronavirus disease of 2019 (COVID-19). The virus invades human cells through the angiotensin-converting enzyme 2 (ACE2) receptor-driven pathway, primarily targeting the human respiratory tract. However, emerging reports of neurological manifestations demonstrate the neuroinvasive potential of SARS-CoV-2. This review highlights the possible routes by which SARS-CoV-2 may invade the central nervous system (CNS) and provides insight into recent case reports of COVID-19-associated neurological disorders, namely ischaemic stroke, encephalitis, encephalopathy, epilepsy, neurodegenerative diseases, and inflammatory-mediated neurological disorders. We hypothesize that SARS-CoV-2 neuroinvasion, neuroinflammation, and blood-brain barrier (BBB) dysfunction may be implicated in the development of the observed disorders; however, further research is critical to understand the detailed mechanisms and pathway of infectivity behind CNS pathogenesis.
Unveiling Human Non-Random Genome Editing Mechanisms Activated in Response to Chronic Environmental Changes: I. Where Might These Mechanisms Come from and What Might They Have Led To?
Authors: Zamai Loris
Published: October 2020

This article challenges the notion of the randomness of mutations in eukaryotic cells by unveiling stress-induced human non-random genome editing mechanisms. To account for the existence of such mechanisms, I have developed molecular concepts of the cell environment and cell environmental stressors and, making use of a large quantity of published data, hypothesised the origin of some crucial biological leaps along the evolutionary path of life on Earth under the pressure of natural selection, in particular, (1) virus–cell mating as a primordial form of sexual recombination and symbiosis; (2) Lamarckian CRISPR-Cas systems; (3) eukaryotic gene development; (4) antiviral activity of retrotransposon-guided mutagenic enzymes; and finally, (5) the exaptation of antiviral mutagenic mechanisms to stress-induced genome editing mechanisms directed at “hyper-transcribed” endogenous genes. Genes transcribed at their maximum rate (hyper-transcribed), yet still unable to meet new chronic environmental demands generated by “pollution”, are inadequate and generate more and more intronic retrotransposon transcripts. In this scenario, RNA-guided mutagenic enzymes (e.g., Apolipoprotein B mRNA editing catalytic polypeptide-like enzymes, APOBECs), which have been shown to bind to retrotransposon RNA-repetitive sequences, would be surgically targeted by intronic retrotransposons on opened chromatin regions of the same “hyper-transcribed” genes. RNA-guided mutagenic enzymes may therefore “Lamarkianly” generate single nucleotide polymorphisms (SNP) and gene copy number variations (CNV), as well as transposon transposition and chromosomal translocations in the restricted areas of hyper-functional and inadequate genes, leaving intact the rest of the genome. CNV and SNP of hyper-transcribed genes may allow cells to surgically explore a new fitness scenario, which increases their adaptability to stressful environmental conditions. Like the mechanisms of immunoglobulin somatic hypermutation, non-random genome editing mechanisms may generate several cell mutants, and those codifying for the most environmentally adequate proteins would have a survival advantage and would therefore be Darwinianly selected. Non-random genome editing mechanisms represent tools of evolvability leading to organismal adaptation including transgenerational non-Mendelian gene transmission or to death of environmentally inadequate genomes. They are a link between environmental changes and biological novelty and plasticity, finally providing a molecular basis to reconcile gene-centred and “ecological” views of evolution.
Editorial Special Issue TGF-Beta/BMP Signaling Pathway
Authors: Fabregat Isabel , Herrera Blanca , Sánchez Aránzazu
Published: October 2020

The transforming growth factor β (TGF-β) superfamily plays key roles in development and tissue homeostasis, controlling the maintenance and regeneration of mature tissues [...]
Autophagy and Lc3-Associated Phagocytosis in Zebrafish Models of Bacterial Infections
Authors: Muñoz-Sánchez Salomé , van der Vaart Michiel , Meijer Annemarie H.
Published: October 2020

Modeling human infectious diseases using the early life stages of zebrafish provides unprecedented opportunities for visualizing and studying the interaction between pathogens and phagocytic cells of the innate immune system. Intracellular pathogens use phagocytes or other host cells, like gut epithelial cells, as a replication niche. The intracellular growth of these pathogens can be counteracted by host defense mechanisms that rely on the autophagy machinery. In recent years, zebrafish embryo infection models have provided in vivo evidence for the significance of the autophagic defenses and these models are now being used to explore autophagy as a therapeutic target. In line with studies in mammalian models, research in zebrafish has shown that selective autophagy mediated by ubiquitin receptors, such as p62, is important for host resistance against several bacterial pathogens, including Shigella flexneri, Mycobacterium marinum, and Staphylococcus aureus. Furthermore, an autophagy related process, Lc3-associated phagocytosis (LAP), proved host beneficial in the case of Salmonella Typhimurium infection but host detrimental in the case of S. aureus infection, where LAP delivers the pathogen to a replication niche. These studies provide valuable information for developing novel therapeutic strategies aimed at directing the autophagy machinery towards bacterial degradation.
Myostatin-1 Inhibits Cell Proliferation by Inhibiting the mTOR Signal Pathway and MRFs, and Activating the Ubiquitin-Proteasomal System in Skeletal Muscle Cells of Japanese Flounder Paralichthys olivaceus
Authors: Liu Jiahuan , Pan Mingzhu , Huang Dong , Guo Yanlin , Yang Mengxi , Zhang Wenbing , Mai Kangsen
Published: October 2020

Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. The mechanisms of fish MSTN involved in muscle growth are not fully understood. In the present study, knockdown and overexpression of mstn-1 was performed in cultured Japanese flounder muscle cells to investigate the molecular function and the underlying mechanism of fish MSTN-1. Results showed that mstn-1 knockdown significantly induced cell proliferation and the mRNA expression of myogenic regulatory factors (MRFs), while overexpression of mstn-1 led to a significant decrease of cell proliferation and a suppression of the MRFs mRNA expression. The overexpression of mstn-1 also significantly increased the mRNA expression of ubiquitin–proteasomal pathway of proteolysis genes including muscle RING-finger protein 1 (murf-1) by 204.1% (p = 0.024) and muscle atrophy F-box protein (mafbx) by 165.7% (p = 0.011). However, mystn-1 overexpression inhibited the activation of mTOR signal pathway and the AKT/FoxO1 pathway through decreasing phosphorylation of AKT at Ser 473 by 56.0% (p = 0.001). Meanwhile, mystn-1 overexpression increased the dephosphorylation and nuclear localization of FoxO1 by 394.9% (p = 0.005). These results demonstrate that mstn-1 in Japanese flounder has the effects of inhibiting cell proliferation and growth, and the mTOR and AKT/FoxO1 pathways participated in these biological effects.
Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research
Authors: Beh Suet Theng , Frisch Carlye , Brafman David A. , Churko Jared , Walker Jessica E. , Serrano Geidy E. , Sue Lucia I. , Reiman Eric M. , Beach Thomas G. , Lue Lih-Fen
Published: October 2020

The Arizona Study of Aging and Neurodegenerative Disorders/Brain and Body Donation Program at Banner Sun Health Research Institute (BSHRI) is a longitudinal clinicopathological study with a current enrollment of more than 900 living subjects for aging and neurodegenerative disease research. Annual clinical assessments are done by cognitive and movement neurologists and neuropsychologists. Brain and body tissues are collected at a median postmortem interval of 3.0 h for neuropathological diagnosis and banking. Since 2018, the program has undertaken banking of scalp fibroblasts derived from neuropathologically characterized donors with Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative diseases. Here, we describe the procedure development and cell characteristics from 14 male and 15 female donors (mean ± SD of age: 83.6 ± 12.2). Fibroblasts from explant cultures were banked at passage 3. The results of mRNA analysis showed positive expression of fibroblast activation protein, vimentin, fibronectin, and THY1 cell surface antigen. We also demonstrated that the banked fibroblasts from a postmortem elderly donor were successfully reprogramed to human-induced pluripotent stem cells (hiPSCs). Taken together, we have demonstrated the successful establishment of a human autopsy-derived fibroblast banking program. The cryogenically preserved cells are available for request at the program website of the BSHRI.
Effect of Graphene Oxide on the Performance of Co-Based Coatings on Ti6Al4V Alloys by Laser Cladding
Authors: Miao Xiaojin , Wu Meiping , Cui Chen , Wang Hang
Published: October 2020

In order to improve the hardness and wear resistance of Ti6Al4V alloys, Co-based coatings with different contents of graphene oxide (GO) were prepared on Ti6Al4V alloys by laser cladding. The effects of the graphene oxide content on the microstructure, hardness, and wear resistance were analyzed. It was found that carbides and undissolved graphene oxide films existed in the coating. Carbides contribute to grain refinement and improve the hardness of the coating. Graphene oxide films can improve the wear resistance of the coating. However, an excessive addition of graphene oxide affects the laser energy absorption of the coating. The results show that when the graphene oxide content is 0.5 wt.%, the performance of the coating is the best. Compared with the Co-based coating without graphene oxide addition, the hardness increased by 32.3%, the friction coefficient decreased by 27.2%, and the wear rate decreased by 66.5%.
Effect of Inorganic Carbon Concentration on the Development of Subaerial Phototrophic Biofilms on Granite
Authors: Vázquez-Nion Daniel , Fuentes Elsa , Prieto Beatriz
Published: October 2020

Organisms living at the stone–air interface are expected to be affected by changes in the atmospheric composition due to greenhouse gases emissions. Increased CO2 concentrations may particularly affect phototrophic microorganisms that colonize stone cultural heritage and form subaerial biofilms. However, little is known about the effects of the environmental changes on microorganisms that colonize stone and the consequences for cultural heritage conservation. In the present study, we investigated how an increase in inorganic carbon concentration affected the development of a subaerial biofilm composed by the cyanobacterium Synechocystis sp. PCC 6803 grown on granite. For this purpose, we established two experiments on biofilm formation, with and without addition of inorganic carbon to the growth medium. Higher concentrations of carbon promoted biofilm growth and increased the concentrations of the photosynthetic pigments chlorophyll a and carotenoids on granite surface, potentially exacerbating the aesthetic impact of these biofilms on stone-made cultural heritage. However, the extracellular polysaccharides produced were not significantly affected by carbon availability, so that physical stone biodeterioration might not be increased by the cyanobacterial matrix. The findings provide valuable data on how the existing global change scenario might affect organisms inhabiting stone cultural heritage and encourage to develop new sustainable treatments and methodologies to prevent biodeterioration and thus preserve stone cultural heritage.
Residual Stresses on Various PVD Hard Coatings on Tube and Plate Substrates
Authors: Lille Harri , Ryabchikov Alexander , Peetsalu Priidu , Lind Liina , Sergejev Fjodor , Mikli Valdek , Kübarsepp Jakob
Published: October 2020

In this study, the average residual stresses were determined in hard PVD nACRo (nc-AlCrN/a-Si3N4), nACo (nc-AlTiN/a-Si3N4), AlCrN, TiAlN, and TiCN commercial coatings through the deflection of the plate substrates and the simultaneous measurement of length variation in thin-walled tubular substrates. The length measuring unit was used for the measurement of any length change in the tubular substrate. A change in tube length was reduced to the deflection of the middle cross-section of the elastic element for which deformation was measured using four strain gauges. The cross-sectional microstructure and thickness of the coatings were investigated by means of scanning electron microscopy (SEM), and a determination was made of the chemical composition of the coatings and substrate by means of energy dispersive X-ray spectroscopy (EDS). The values of average compressive residual stresses, as determined by both methods, were very high (with a variation of between 2.05 and 6.63 GPa), irrespective of coating thickness, but were dependent upon the shape of the substrate and on its position in relation to the axis of the rotating cathode. The thicknesses of the coatings that were deposited on the plates with two parallel fixings (such as the nACRo coatings on the front surface at 6.8 μm and on the rear surface at 2.9 μm) and on the tubular substrates (10.0 μm) were significantly different. The higher average compressive residual stresses in the coating correlate to the higher average relative wear resistance that was obtained during field wear testing.
Adsorption of Mixtures of a Pegylated Lipid with Anionic and Zwitterionic Surfactants at Solid/Liquid
Authors: Llamas Sara , Guzmán Eduardo , Ortega Francisco , Rubio Ramón G.
Published: October 2020

This work explores the association of a pegylated lipid (DSPE-PEG) with different anionic and zwitterionic surfactants (pseudo-binary and pseudo-ternary polymer+ surfactant mixtures), and the adsorption of the polymer + surfactant aggregates onto negatively charged surfaces, with a surface charge density similar to that existing on the damaged hair epicuticle. Dynamic light scattering and zeta potential measurements shows that, in solution, the polymer + surfactant association results from an intricate balance between electrostatic and hydrophobic interactions, which leads to the formation of at least two different types of micellar-like polymer + surfactant aggregates. The structure and physicochemical properties of such aggregates were found strongly dependent on the specific nature and concentration of the surfactant. The adsorption of the polymer + surfactant aggregates onto negatively charged surface was studied using a set of surface-sensitive techniques (quartz crystal microbalance with dissipation monitoring, ellipsometry and Atomic Force Microscopy), which allows obtaining information about the adsorbed amount, the water content of the layers and the topography of the obtained films. Ion-dipole interactions between the negative charges of the surface and the oxyethylene groups of the polymer + surfactant aggregates appear as the main driving force of the deposition process. This is strongly dependent on the surfactant nature and its concentration, with the impact of the latter on the adsorption being especially critical when anionic surfactant are incorporated within the aggregates. This study opens important perspectives for modulating the deposition of a poorly interacting polymer onto negatively charged surfaces, which can impact in the fabrication on different aspects with technological and industrial interest.
Microstructure and Fluctuation-Induced Conductivity Analysis of Bi2Sr2CaCu2O8+δ (Bi-2212) Nanowire Fabrics
Authors: Koblischka Michael , Koblischka-Veneva Anjela , Zeng XianLin , Hannachi Essia , Slimani Yassine
Published: October 2020

Resistance measurements were performed on Bi2Sr2CaCu2O8+δ (Bi-2212) fabric-like nanowire networks or nanofiber mats in the temperature interval 3 K ≤T≤ 300 K. The nanowire fabrics were prepared by means of electrospinning, and consist of long (up to 100 μm) individual nanowires with a mean diameter of 250 nm. The microstructure of the nanowire network fiber mats and of the individual nanowires was thoroughly characterized by electron microscopy showing that the nanowires can be as thin as a single Bi-2212 grain. The polycrystalline nanowires are found to have a texture in the direction of the original polymer nanowire. The overall structure of the nanofiber mats is characterized by numerous interconnects among the nanowires, which enable current flow across the whole sample. The fluctuation-induced conductivity (excess conductivity) above the superconducting transition temperature, Tc, was analyzed using the Aslamzov-Larkin model. Four distinct fluctuation regimes (short-wave, two-dimensional, three-dimensional and critical fluctuation regimes) could be identified in the Bi-2212 nanowire fabric samples. These regimes in such nanowire network samples are discussed in detail for the first time. Based on this analysis, we determine several superconducting parameters from the resistance data.
A New Pedagogy to Enhance the Safety and Resilience of Journalists in Dangerous Environments Globally
Authors: Murphy Colm , Deeny Pat , Taylor Nigel
Published: October 2020

Risks to journalists are rising with disasters, epidemics, physical, mental and digital harassment all increasing globally. Some 1382 journalists have been killed since 1992 and 246 are imprisoned. However, the threat type has been changing, with the majority of journalists killed annually being ones working in their own country, often who are targeted for assassination. In response, UNESCO and others have called for research into best practice for safety education to halt this and the consequential decline in global media freedom. This five-year award winning project, A Holistic Humanitarian Approach to Enhance the Safety and Resilience of Journalists Globally, tested the hypothesis that a new pedagogy based on a ‘holistic humanitarian’ philosophy would be more effective in protecting journalists working in dangerous domains globally than existing provisions. The little-changed 30-year-old dominant international provision, the ‘military battlefield’ pedagogy, is used by the world’s major news organizations like BBC, CNN and the New York Times. This new pedagogy adapted and customized best practice from other professions and used Taylor’s 2020 Competencies for Disaster Healthcare professionals. A new program was devised and the two international cohorts who took it in 2018 and 2019 judged that it ‘very significantly’ enhanced their resilience and safety skills. Its concentration on group and individual physical and mental resilience building, risk mitigation, psychology, communication, self-defence, and digital security skill acquisition was a paradigm shift in training internationally for news professionals in dangerous environments. The research, thus, proved the study’s hypotheses.
The Clean Energy Package and Demand Response: Setting Correct Incentives
Authors: Willems Bert , Zhou Juulia
Published: October 2020

We describe how recent EU regulation affects demand response (DR) and highlight some of the remaining regulatory challenges from a legal and economic viewpoint. With the Clean Energy Package (CEP), the EU has opted for a fully market-based, consumer-centered approach for DR. The development of business models and products is left to a large extent to market forces. However, to enable the efficient development of those DR markets, network regulation has to adapt. (1) Network tariffs have to become more cost-reflective to provide correct incentives to market participants. The capacity tariffs have to increase, net-metering should be abolished, and optional tariff components for providing flexibility may need to be considered. (2) The regulation for distribution system operators (DSOs) may need to be fine-tuned to reflect their new roles. We present three scenarios: (a) a horizontal merger of unbundled DSOs under incentive regulation, (b) a DSO as a subsidiary of an integrated utility under cost plus regulation, (c) a transfer of some activities from DSO to TSO.
Effect of Martensitic Transformation and Grain Size on the Surface Roughening Behavior in SUS 304 and SUS 316 Thin Metal Foils
Authors: Aziz Abdul , Yang Ming
Published: October 2020

The surface roughening and martensitic phase transformation (MPT) of SUS 304 and SUS 316 were studied through two experiments: a uniaxial tensile stress test, repeated for five cycles, and an Scanning Electron Microscope–Electron Backscaterr Diffraction (SEM-EBSD) investigation. The MPT and martensitic volume fraction (Mf) were evaluated following the tensile test. The correlation between MPT, Mf, and surface roughening behavior was investigated. The experiment showed that an increase in the strain level from 0.4% to 1% increased the MPT and Mf, which transformed from a metastable austenitic phase in SUS 304. The increased strain level increased the surface roughening for various grain sizes (Dg), from fine grain (Dg < 3 μm) to coarse grain (Dg ≥ 3 μm). SUS 304 and SUS 316 are used so that the surface roughening mechanism between SUS 304 and SUS 316, with different phase conditions and at a similar Dg, can be determined. The results showed that the surface roughening increased for both fine and coarse grain at strain levels of 0.4% and 1%; however, a larger increase of surface roughening was obtained for coarse grain. In coarse grain, surface roughening increased significantly not only with a low MPT, but also with a low grain deformation. In coarse grain, the surface roughening increased proportionally to the strain level (εp) because of the low MPT and weak grain. In fine grain, the surface roughening did not increase proportionally to the εp because of the high MPT, which increased the grain strength in SUS 304. In the fine grain of SUS 304, the increase of surface roughening was nearly the same both at strain levels of 0.4% and 1%, because the MPT and Mf were nearly the same. The surface roughening with the same εp and almost the same Dg in SUS 304 and SUS 316 fine grain was nearly the same, because the grain deformation almost produced the same relative inclination between neighboring grains in the initial state direction to the surface. The inter-grain movement changed the grain orientation. Based on kernel average misorientation (KAM) mapping, the local grain misorientation in SUS 304 was higher than that in SUS 316. This indicated that the fine-grain SUS 304 is harder than the fine-grain SUS 316. There is no MPT in SUS 316 because of the higher austenitic phase, which is affected by the austenitic former, such as Ni.
Association of Recent Incidence of Foliar Disease in Pine Species in the Southeastern United States with Tree and Climate Variables
Authors: Pandit Karun , Smith Jason , Quesada Tania , Villari Caterina , Johnson Daniel J.
Published: October 2020

Pine forests in the southern United States are a major contributor to the global economy. Through the last three decades, however, there have been concerns about the decline of pine forests attributed mostly to pests and pathogens. A combination of biotic agents and environmental factors and their interaction often influences outbreaks and the resultant damage in the forests. Southern pines experience periodic mortality from bark beetles and root rot fungi and losses from fusiform rust and pitch canker have long been important for management. In recent years, there is also growing evidence of increasing damage from foliar disease in southern pines. Early detection of diseases following changes in foliar characteristics and assessment of potential risks will help us better utilize our resources and manage these forests sustainably. In this study, we used Forest Inventory and Analysis (FIA) data to explore the intensity of foliar disease in three common pines: loblolly (Pinus taeda L.), longleaf (Pinus palustris Mill.), and slash (Pinus elliottii Engelm.) in spatial and temporal terms using tree-level and climatic variables. Results from a tree-level model suggests that crown ratio may be an important factor in pine foliar disease (p < 0.1). We applied the MaxEnt model, a presence-only species distribution model (SDM), to explore any association of foliar disease incidences with the climatic variables at a landscape level. Results indicate that mean dew point temperature, maximum vapor pressure deficit, and precipitation during cold months had more influence over disease incidences than other climatic variables. While the sample size is limited as this is an emerging disease in the region, our study provides a basis for further exploration of disease detection methods, disease etiology studies, and hazard mapping.
Thermal Performance of a Heated Pipe in the Presence of a Metal Foam and Twisted Tape Inserts
Authors: Papazian K. , Al Hajaj Z. , Saghir M. Z.
Published: October 2020

To meet the demand for more efficient ways of cooling and heating, new designs and further development of heat exchangers is essential in industry. The present study focuses on the thermal performance of a circular pipe with two inserts. The first insert consists of a porous medium having a porosity of 0.91, and the second one consists of a single twist solid insert. Different ranges of heating conditions have been applied for different flow rates. Water and titanium dioxide (TiO2) nanofluid 1% vol are the liquid media used for cooling. Laminar flow is assumed for two different Reynolds numbers of 1000 and 2000. The results of the study have shown that the twisted tape insert increases the thermal efficiency of the pipe more than the porous media insert and the plain pipe. In addition, different temperature readings in the cross section of the pipe have indicated that the twisted tape helps mixing up the fluid and provides a constant temperature in the overall volume of the fluid, whereas for the porous media insert and plain pipe the fluid temperature increases in the fluid particles close to the pipe inner surface. TiO2 nanofluid exhibited an enhancement when compared to water for a plain and porous pipe. However, this enhancement was absent when a twisted insert is used.
Advances of Spectrometric Techniques in Food Analysis and Food Authentication Implemented with Chemometrics
Authors: Karabagias Ioannis K.
Published: October 2020

Given the continuous consumer demand for products of high quality and specific origin, there is a great tendency for the application of multiple instrumental techniques for the complete characterization of foodstuffs or related natural products. Spectrometric techniques usually offer a full and rapid screenshot of products’ composition and properties by the determination of specific bio-molecules such as sugars, minerals, polyphenols, volatile compounds, amino acids, organic acids, etc. The present special issue aimed firstly to enhance the advances of the application of spectrometric techniques such as gas chromatography coupled to mass spectrometry (GC-MS), inductively coupled plasma optical emission spectrometry (ICP-OES), isotope ratio mass spectrometry (IRMS), nuclear magnetic resonance (NMR), Raman spectroscopy, or any other spectrometric technique, in the analysis of foodstuffs such as meat, milk, cheese, potatoes, vegetables, fruits/fruit juices, honey, olive oil, chocolate, and other natural products. An additional goal was to fill the gap between food composition/food properties/natural products properties and food/natural products authenticity, using supervised and non-supervised chemometrics. Of the 18 submitted articles, nine were eventually published, providing new information to the field.
Tomato Oil Encapsulation by α-, β-, and γ-Cyclodextrins: A Comparative Study on the Formation of Supramolecular Structures, Antioxidant Activity, and Carotenoid Stability
Authors: Durante Miriana , Milano Francesco , Caroli Monica De , Giotta Livia , Piro Gabriella , Mita Giovanni , Frigione Mariaenrica , Lenucci Marcello Salvatore
Published: October 2020

Cyclodextrins (CDs) are oligosaccharides, comprising 6 (α), 7 (β), or 8 (γ) glucose residues, used to prepare oil-in-water emulsions and improve oil stability towards degradation. In this research, the aptitude of α-, β-, and γ-CDs to form complexes with a supercritical CO2 extracted lycopene-rich tomato oil (TO) was comparatively assessed. TO/CD emulsions and the resulting freeze-dried powders were characterized by microscopy, Fourier transform infrared-attenuated total reflection (FTIR-ATR), and differential scanning calorimetry (DSC), as well as for their antioxidant activity. Furthermore, carotenoid stability was monitored for 90 days at 25 and 4 °C. Confocal and SEM microscopy revealed morphological differences among samples. α- and β-CDs spontaneously associated into microcrystals assembling in thin spherical shells (cyclodextrinosomes, Ø ≈ 27 µm) at the oil/water interface. Much smaller (Ø ≈ 9 µm) aggregates were occasionally observed with γ-CDs, but most TO droplets appeared “naked”. FTIR and DSC spectra indicated that most CDs did not participate in TO complex formation, nevertheless structurally different interfacial complexes were formed. The trolox equivalent antioxidant capacity (TEAC) activity of emulsions and powders highlighted better performances of α- and β-CDs as hydrophobic antioxidants-dispersing agents across aqueous media. Regardless of CDs type, low temperature slowed down carotenoid degradation in all samples, except all-[E]-lycopene, which does not appear efficiently protected by any CD type in the long storage period.
Potentials and Safety of Date Palm Fruit against Diabetes: A Critical Review
Authors: Mia Md Al-Tareq , Mosaib Md Golam , Khalil Md Ibrahim , Islam Md Asiful , Gan Siew Hua
Published: October 2020

Diabetes is a chronic metabolic disorder triggered by disturbances in carbohydrate, protein, and lipid metabolisms, where either reduced secretion or sensitivity of insulin is observed coupled with poor glucose control. Date palm fruits are one of the fruits reported to have good potential in diabetes treatment due to its presence of polyphenols exerting strong antioxidant activities. Other possible mechanisms of action include the polyphenolic compounds, which can inhibit enzymes like α-amylase and α-glucosidase. Flavonoids in dates can stimulate β-cells by increasing the number of islets and β-cells, recovering endocrine pancreatic tissues, reducing β-cell apoptosis, activating insulin receptors following the increase in insulin secretion, in addition to improving diabetes-induced complications. In this review, the in vitro, in vivo, and human study-based evidence of date palm as an anti-diabetic fruit is summarised.
Strategies for Microbial Decontamination of Fresh Blueberries and Derived Products
Authors: Pérez-Lavalle Liliana , Carrasco Elena , Valero Antonio
Published: October 2020

Increasing consumption of blueberries is associated with appreciation of their organoleptic properties together with their multiple health benefits. The increasing number of outbreaks caused by pathogenic microorganisms associated with their consumption in the fresh state and the rapid spoilage of this product which is mainly caused by moulds, has led to the development and evaluation of alternatives that help mitigate this problem. This article presents different strategies ranging from chemical, physical and biological technologies to combined methods applied for microbial decontamination of fresh blueberries and derived products. Sanitizers such as peracetic acid (PAA), ozone (O3), and electrolyzed water (EOW), and physical technologies such as pulsed light (PL) and cold plasma (CP) are potential alternatives to the use of traditional chlorine. Likewise, high hydrostatic pressure (HHP) or pulsed electrical fields (PEF) successfully achieve microbial reductions in derivative products. A combination of methods at moderate intensities or levels is a promising strategy to increase microbial decontamination with a minimal impact on product quality.
Aroma, Quality, and Consumer Mindsets for Shelf-Stable Rice Thermally Processed by Reciprocal Agitation
Authors: Dixon William R. , Morales-Contreras Blanca E. , Kongchum Manoch , Xu Zhimin , Harrell Dustin , Moskowitz Howard R. , Wicker Louise
Published: October 2020

Food engineering, food chemistry, and consumer segmentation were used to evaluate ready-to-eat rice. The aromatic Louisiana Clearfield Jazzman (CJ) and Thai Jasmine (TJ), and a non-aromatic parboiled (PB) rice were hydrated during the first 10 min of processing with reciprocal agitation followed by static retort processing. The aroma compound, 2-Acetyl-1-pyrroline (2-AP) was more heat-stable in CJ than TJ rice but decreased 15-fold compared to the rice cooker method. Pareto analysis indicated that rice type and agitation had the main effect on amylose and total starch and chroma and hue. Color differences of rice agitated during hydration and between rice cooker or static retort processed rice, indicated only slight differences for each rice variety. Hydration of dry rice during retort cooking and similar starch, color, and aroma quality were achieved with reciprocal compared to static or rice cooker methods. Survey responses categorized consumers into three, mindsets driven by rice consumption, convenience, or packaging.
Cricket-Enriched Oat Biscuit: Technological Analysis and Sensory Evaluation
Authors: Biró Barbara , Sipos Mária Anna , Kovács Anikó , Badak-Kerti Katalin , Pásztor-Huszár Klára , Gere Attila
Published: October 2020

Insect-containing products are gaining more space in the market. Bakery products are one of the most promising since the added ground insects can enhance not only the nutritional quality of the dough, but technological parameters and sensory properties of the final products. In the present research, different amounts of ground Acheta domesticus (house cricket) were used to produce oat biscuits. Colour, hardness, and total titratable acidity (TTA) values were measured as well as a consumer sensory test was completed using the check-all-that-apply (CATA) method. An estimation of nutrient composition of the samples revealed that, according to the European Union’s Regulation No. 1924/2006, the products with 10 and 15 g/100 g cricket enrichment (CP10 and CP15, respectively) can be labelled as protein sources. Results of the colour, TTA, and texture measurements showed that even small amounts of the cricket powder darkened the colour of the samples and increased their acidity, but did not influence the texture significantly. Among product-related check all that apply (CATA) attributes, fatty and cheesy flavour showed a significant positive effect on overall liking (OAL). On the other hand, burnt flavour and brown colour significantly decreased OAL. OAL values showed that consumers preferred the control product (CP0) and the product with 5 g/100 g cricket enrichment (CP5) samples over CP10 and rejected CP15.
Effects of Radishes, Apples, and Pears on the Lactic Acid Bacteria and Nutritional and Functional Qualities of Flavored Soy Sauce
Authors: Bahuguna Ashutosh , Jo Il Guk , Lee Jong Suk , Kim Myunghee
Published: October 2020

Producers of soy sauce are constantly making efforts to improve the sensory quality and nutritional value of their products. In this study, radishes, apples, and pears were used to prepare a distinctly flavored soy sauce, and the lactic acid bacteria, volatile compound content, and nutritional and functional qualities of the product were compared with two commercial flavored soy sauce products. Comparable physiochemical properties, antioxidant activities (in vitro and cellular), and higher prevalence of lactic acid bacteria (7.74 ± 0.55 log CFU mL−1) were observed in the prepared flavored soy sauce than in commercial flavored soy sauce. The comprehensive enzyme activity profile of the isolated lactic acid bacteria, Tetragenococcus halophilus (NCBI GenBank Accession no. MN270899), revealed the absence of any harmful enzymes such as β-glucuronidase. Moreover, the cell-free extract of T. halophilus showed xanthine oxidase inhibitory activity (half maximal inhibitory concentration (IC50) = 0.79 mg mL−1), suggesting that the product possessed functionality against xanthine oxidase-induced oxidative stress. Additionally, the prepared flavored soy sauce had higher amounts of total free amino acids (48.68 mg mL−1) and organic acids (7.77 mg mL−1). These results suggest that radishes, apples, and pears at a defined ratio are suitable for the large scale production of a flavored soy sauce with improved nutritional and functional qualities.
Preliminary Assessment of Parmigiano Reggiano Authenticity by Handheld Raman Spectroscopy
Authors: Li Vigni Mario , Durante Caterina , Michelini Sara , Nocetti Marco , Cocchi Marina
Published: October 2020

Raman spectroscopy, and handheld spectrometers in particular, are gaining increasing attention in food quality control as a fast, portable, non-destructive technique. Furthermore, this technology also allows for measuring the intact sample through the packaging and, with respect to near infrared spectroscopy, it is not affected by the water content of the samples. In this work, we evaluate the potential of the methodology to model, by multivariate data analysis, the authenticity of Parmigiano Reggiano cheese, which is one of the most well-known and appreciated hard cheeses worldwide, with protected denomination of origin (PDO). On the other hand, it is also highly subject to counterfeiting. In particular, it is critical to assess the authenticity of grated cheese, to which, under strictly specified conditions, the PDO is extended. To this aim, it would be highly valuable to develop an authenticity model based on a fast, non-destructive technique. In this work, we present preliminary results obtained by a handheld Raman spectrometer and class-modeling (Soft Independent Modeling of Class Analogy, SIMCA), which are extremely promising, showing sensitivity and specificity of 100% for the test set. Moreover, another salient issue, namely the percentage of rind in grated cheese, was addressed by developing a multivariate calibration model based on Raman spectra. It was possible to obtain a prediction error around 5%, with 18% being the maximum content allowed by the production protocol.
Formulation of New Baking (+)-Catechin Based Leavening Agents: Effects on Rheology, Sensory and Antioxidant Features during Muffin Preparation
Authors: Carullo Gabriele , Scarpelli Francesca , Belsito Emilia Lucia , Caputo Paolino , Oliviero Rossi Cesare , Mincione Antonio , Leggio Antonella , Crispini Alessandra , Restuccia Donatella , Spizzirri Umile Gianfranco , Aiello Francesca
Published: October 2020

The aim of this investigation was to prepare two solid mixtures containing a soluble polymorph of (+)-catechin and mucic (MUC) or tartaric (TAR) acids as new leavening agents. The solid mixtures were based on a polymorph of (+)-catechin, characterized through Powder X-ray Diffraction (PXRD) analysis and assayed in in vitro antioxidant and solubility assays. The dough samples were studied by dynamic rheological tests, while muffins were studied through Headspace Solid-Phase Microextraction (HS-SPME)/Gas Chromatography-Mass Spectrometry (GC-MS) analysis to identify volatile compounds, in vitro tests to evaluate antioxidant properties, and sensory analyses. TAR powder showed a solubility in water almost one order of magnitude increased with respect to commercial (+)-catechin (40.0 against 4.6 mg mL−1) and increased antioxidant performances. In particular, TAR showed total phenolic content (TPC) and total antioxidant capacity (TAC) values of 0.0298 ± 0.021 and 0.0081 ± 0.0009 meq CT/g, while MUC showed better results in terms of 2,2-diphenyl-1-picrylhydrazyl) acid (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 0.518 ± 0.015 and 0.112 ± 0.010 mg/mL, respectively. MS analysis identified different compounds derived from the lipid oxidation process. Muffins obtained using both powders showed interesting outcomes regarding dough process and appreciable appearance/olfactory/taste/texture profiles. Muffins obtained from TAR-based mixture showed also a total phenolic content of 0.00175 meq CT/g muffin, and almost two times improved TAC and scavenger activity against DPPH radical. The formulated powders could be used as suitable health-promoting ingredients in the food industry.
Relationships between Emotion, Acceptance, Food Choice, and Consumption: Some New Perspectives
Authors: Prinyawiwatkul Witoon
Published: October 2020

Food is more than just a source of nutrients—it also provides basic pleasure as well as aesthetic experiences. A number of studies have reported that acceptance, food choice, and consumption are affected by a large number of factors, including both intrinsic and extrinsic factors and cues, as well as consumer characteristics. Food-elicited emotions are becoming a critical component in designing products that meet consumers’ needs and expectations. Several studies have reported emotional responses to food and their relationships to product acceptability, preference, and choice. This Special Issue brings together a small range of studies with a diversity of approaches that provide good examples of the complex and multidisciplinary nature of the subject matter.
In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes
Authors: Fernández-Fernández Adriana Maite , Iriondo-DeHond Amaia , Nardin Tiziana , Larcher Roberto , Dellacassa Eduardo , Medrano-Fernandez Alejandra , Castillo María Dolores del
Published: October 2020

Diabetes pathogenesis encompasses oxidative stress, inflammation, insulin malfunctioning and partial or total insulin secretion impairment, which leads to a constant hyperglycemia. Polyphenols are known to possess bioactive properties, being Tannat grape skin a natural and sustainable source of these compounds. The present study aimed to find out the bioaccessibility of health-promoting molecules composing a multifunctional extract from Tannat grape skin obtained under hydro-alcoholic-acid conditions. The identification of phenolic compounds in the samples was performed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Subsequently, the samples were in vitro digested mimicking the human oral gastrointestinal conditions and the bioactivity of the digest (antioxidant, anti-inflammatory and modulation of glucose metabolism) was assessed. Effect on glucose metabolism was estimated by measuring carbohydrases activity and the functionality of glucose transporters of small intestine cells in presence and absence of the digested extract. Flavonoids, phenolic acids and phenolic alcohols were the major phenol compounds detected in the extract. The bioaccessible compounds protected the intestinal cells and macrophages against the induced formation of reactive oxygen species (ROS) and nitric oxide (NO). In addition, glucose transporters were inhibited by the digested extract. In conclusion, the bioaccessible compounds of the extract, including phenols, modulated key biochemical events involved in the pathogenesis of diabetes such as oxidative stress, inflammation and glucose absorption. The extract was effective under prevention with co-administration conditions supporting its potential for either reducing the risk or treating this disease.
A Note on Connectivity and Stability in Dynamic Network Formation
Authors: Jun Tackseung , Kim Jeong-Yoo
Published: October 2020

We consider the dynamic network formation problem under the requirement that the whole network be connected and remain connected after q nodes are destroyed. We propose the concept of dynamic Cq-stability and characterize dynamic Cq-stable networks for any q≥0. Comparison with the outcome in the static model is also discussed.