bluuebunny/crossref_metadata_2025 huggingface dataset:
Removal and Recovery of Dissolved Oil from High-Salinity Wastewater Using Graphene–Iron Oxide Nanocomposites
Authors: Diraki Ahmad , Mackey Hamish R. , McKay Gordon , Abdala Ahmed
Published: September 2022

We report the synthesis of reduced graphene oxide (rGO)-α-Fe2O3 nanocomposite and its application to remove and recover dissolved oil from a high-salinity oil–water emulsion in batch and column/breakthrough setups. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and nitrogen adsorption characterized the synthesized nanocomposite’s structure, morphology, and surface properties. Both batch and continuous breakthrough adsorption studies were investigated. The effect of the adsorption parameters on the adsorption capacity and removal efficiency was analyzed. The rGO-Fe2O3 nanocomposite (rGO-Fe2O3-NC) demonstrated a superior adsorption capacity, both when measured experimentally (1213 mg/g) and predicted using the Freundlich isotherm (1301 mg/g). The adsorption process followed pseudo-second-order kinetic, and the rGO-Fe2O3-NC exhibited a very rapid removal, with more than 60% of oil being removed within 10 min. Breakthrough confirmed the exceptional removal capacities with good regeneration and cycling ability under a short contact time. Moreover, the adsorption capacity was enhanced with an emulsion salinity of up to 100,000 ppm, confirming the suitability for high-salinity wastewater.
Temperature Effect on Electrical Resistivity Measurement Using an Embedded Sensor to Estimate Concrete Water Content
Authors: Badr Joanna , Mahfoud Elie , Villain Géraldine , Balayssac Jean-Paul , Palma Lopes Sérgio , Fargier Yannick , Yven Béatrice
Published: September 2022

Concrete resistivity measurements strongly depend on the temperature and the water content of the structure. In this paper, a study of the effect of the temperature and saturation degree on electrical resistivity measurement is carried out using an embedded printed circuit board sensor to estimate water content profiles in concrete structures. Resistivity measurements are performed at temperatures between 20 and 60 °C. Experimental results are presented and analyzed in light of well-established empirical models. Calibration curves that link the electrical resistivity to the degree of saturation at a given temperature are discussed. Arrhenius laws that depend on the degree of saturation can be used to fit our data. In the perspective of the instrumentation and monitoring of concrete structure in real conditions, it is important to master the temperature correction laws of resistivity measurement to evaluate the gradients of water saturation degree.
Theoretical and Experimental Comparisons of Total Solar Transmittance for Polycarbonate Sheet with Twin Wall Rectangular Structure
Authors: Zapałowicz Zbigniew , Garnysz-Rachtan Agnieszka
Published: September 2022

Multiwall polycarbonate sheets are applied as construction elements. Modelling and analysis of thermal processes that occur in this material demand the knowledge of solar transmittance. Values of this parameter determined in laboratory conditions are given in the technical specification of the product. However, the parameter is in practice a complex function depending on the number of factors. This paper presents theoretical and experimental research results for total solar transmittance (TST) for a polycarbonate sheet with twin wall rectangular structure. Theoretical TST is calculated as a product of transmissivity after accounting for light absorption in polycarbonate and of transmissivity after accounting for multiple reflections of solar rays from walls of a channel. The first kind of transmissivity is insignificant and can be neglected. The second one depends on the number of reflection layers, season, and time of day. Experimental TST is determined as the ratio of irradiance under and above the polycarbonate sheet measured by pyranometers. Experimental TST is also a function of time of day and season. Both kinds of TST have an approximately constant value in the time about noon. The theoretical values of TST (0.74) are approximately equal to experimental values of TST (0.75) for the selected summer day. The value of TST in catalogue is equal to 0.82.
Estimating FAO Blaney-Criddle b-Factor Using Soft Computing Models
Authors: Thongkao Suthira , Ditthakit Pakorn , Pinthong Sirimon , Salaeh Nureehan , Elkhrachy Ismail , Linh Nguyen Thi Thuy , Pham Quoc Bao
Published: September 2022

FAO Blaney-Criddle has been generally an accepted method for estimating reference crop evapotranspiration. In this regard, it is inevitable to estimate the b-factor provided by the Food and Agriculture Organization (FAO) of the United Nations Irrigation and Drainage Paper number 24. In this study, five soft computing methods, namely random forest (RF), M5 model tree (M5), support vector regression with the polynomial function (SVR-poly), support vector regression with radial basis function kernel (SVR-rbf), and random tree (RT), were adapted to estimate the b-factor. And Their performances were also compared. The suitable hyper-parameters for each soft computing method were investigated. Five statistical indices were deployed to evaluate their performance, i.e., the coefficient of determination (r2), the mean absolute relative error (MARE), the maximum absolute relative error (MXARE), the standard deviation of the absolute relative error (DEV), and the number of samples with an error greater than 2% (NE > 2%). Findings reveal that SVR-rbf gave the highest performance among five soft computing models, followed by the M5, RF, SVR-poly, and RT. The M5 also derived a new explicit equation for b estimation. SVR-rbf provided a bit lower efficacy than the radial basis function network but outperformed the regression equations. Models’ Applicability for estimating monthly reference evapotranspiration (ETo) was demonstrated.
Testing Filter-Based Air Cleaners with Surrogate Particles for Viruses and Exhaled Droplets
Authors: Schumacher Stefan , Banda Sanchez Arantxa , Caspari Anna , Staack Katharina , Asbach Christof
Published: September 2022

Indoor air cleaners can contribute to reducing infection risks by the filtration of virus-carrying droplets. There are various national standards to test indoor air cleaners that determine the clean air delivery rate (CADR), but typically only as a size-integrated value for particles > 0.3 μm. Thus, a test method using potassium chloride (KCl) and paraffin as surrogate particles in the size range of viruses and exhaled droplets was developed. We show that air cleaners with fibrous and electrostatic filters are generally capable of reducing the airborne particle concentrations. However, for electret filters, the performance can strongly degrade over time by being loaded with particles. By comparing filters with different efficiencies in the same air cleaner, we demonstrate that the use of high-efficiency filters can be even at the expense of the cleaning efficacy. We developed a mathematical model to estimate the inhaled dose of viruses and show that the combination of natural venting and an air cleaner can lead to a substantial reduction of the infection risk.
Influence of the Geographic Channel Effect on PM2.5 Concentrations over the Taipei Basin in Relation to Continental High-Pressure Systems during Winter
Authors: Lai Li-Wei , Lin Chuan-Yao
Published: September 2022

In addition to the Taiwan Strait, the geographical channel effect (GCE) has been observed in the Taipei Basin during winters. This study explored the potential links amongst GCE phenomena in the basin, fine particulate matter (PM2.5) events, and position of the continental high-pressure system (CHPS). Principal component analysis was applied to classify the CHPS distribution patterns influencing PM2.5 concentrations and meteorological parameters in the Taipei metropolis. In addition, non-parametric Kruskal–Wallis and Dwass–Steel–Critchlow–Fligner tests were applied to compare the parameters amongst distinct groups. Moreover, track analysis was utilised to trace the trajectories of air masses. The merged Dark Target Aerosol Angstrom Exponent (Ocean), Deep Blue Aerosol Angstrom Exponent (Land), and Aerosol Optical Depth (AOD) 3 km (Land and Ocean) of Terra/MODIS and Aqua/MODIS were used to confirm the connection of long-range PM2.5 transport. Two features in the atmospheric environment induce double GCEs: (1) wind direction below 90° in most of the upwind area of the Taisumi and Xindian River Basins; (2) CHPS centres moved across 110° E. Double GCEs can reduce the magnitude of PM2.5 air pollution and shape the distribution of PM2.5; however, they cannot prevent PM2.5 events.
Long-Term Outcome of Leptospirosis Infection with Acute Kidney Injury
Authors: Chang Chih-Hsiang , Sun Wei-Chiao , Chang Su-Wei , Lee Cheng-Chia , Fan Pei-Chun , Yang Huang-Yu , Yang Chih-Wei
Published: September 2022

Acute kidney injury (AKI) is associated with long-term mortality and morbidity outcomes. Severe leptospirosis usually results in AKI and multiple organ failure, but is associated with favorable short-term outcomes, if treatment is initiated early. However, information on long-term outcomes after leptospirosis-associated AKI is limited. The effects of leptospirosis on resulting chronic kidney disease (CKD), as well as mortality, were evaluated in this study. We studied 2145 patients with leptospirosis from the National Health Insurance Research Database over an 8-year follow-up period. Patient demographics and characteristics were analyzed for AKI and dialysis. The risk factors for renal outcomes were analyzed using multivariate logistic regression. In total, 443 (20.6%) patients had AKI. Among them, 77 (3.6%) patients received replacement therapy (AKI-RRT group). Long-term mortality was higher in the AKI-RRT group than in the AKI group and non-AKI group, based on a multivariate logistic regression model. Similarly, the incidence rate of CKD was highest in the AKI-RRT group, followed by the AKI and non-AKI groups. Leptospirosis, complicated with AKI, may play a critical role in the long-term outcomes, resulting in CKD. The severity of AKI determines the incidence of CKD. Additional prospective investigations for the early detection of AKI in leptospirosis are warranted.
Dermatological Autoimmune Considerations of Immune Checkpoint Therapy
Authors: Fane Lauren S. , Efird Jimmy T. , Jindal Charulata , Biswas Tithi
Published: September 2022

The most common immune-related adverse events (irAEs) involve the skin, and several serve as predictors of response to immune checkpoint inhibitor (ICI) therapy, especially in melanoma. Patients with pre-existing skin autoimmune diseases (ADs) have been excluded from ICI studies for safety concerns, yet recent research has shown that dermatological ADs can be managed without discontinuing ICI therapy. Patients with ADs respond as well or better to ICIs and can be included as candidates in clinical trials. Frequently taken during ICI therapy, steroids impair immunotherapy efficacy in certain anatomical sites of tumors but not others, including the brain. ICI efficacy can be enhanced by radiotherapy without increasing adverse events, as neoadjuvant radiotherapy is thought to sensitize tumors to ICIs. This perspective highlights clinical autoimmune considerations of ICI therapy in melanoma and discusses important areas for future exploration.
Imaging Effector Memory T-Cells Predicts Response to PD1-Chemotherapy Combinations in Colon Cancer
Authors: Goggi Julian L. , Khanapur Shivashankar , Hartimath Siddesh V. , Ramasamy Boominathan , Cheng Peter , Chin Hui-Xian , Tang Jun-Rong , Hwang You-Yi , Robins Edward G.
Published: September 2022

Often, patients fail to respond to immune checkpoint inhibitor (ICI) treatment despite favourable biomarker status. Numerous chemotherapeutic agents have been shown to promote tumour immunogenicity when used in conjunction with ICIs; however, little is known about whether such combination therapies lead to a lasting immune response. Given the potential toxicity of ICI–chemotherapy combinations, identification of biomarkers that accurately predict how individuals respond to specific treatment combinations and whether these responses will be long lasting is of paramount importance. In this study, we explored [18F]AlF-NOTA-KCNA3P, a peptide radiopharmaceutical that targets the Kv1.3 potassium channel overexpressed on T-effector memory (TEM) cells as a PET imaging biomarker for lasting immunological memory response. The first-line colon cancer chemotherapies oxaliplatin and 5-fluorouracil were assessed in a syngeneic colon cancer model, either as monotherapies or in combination with PD1, comparing radiopharmaceutical uptake to memory-associated immune cells in the tumour. [18F]AlF-NOTA-KCNA3P reliably separated tumours with immunological memory responses from non-responding tumours and could be used to measure Kv1.3-expressing TEM cells responsible for durable immunological memory response to combination therapy in vivo.
Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis
Authors: Ebrahimi Rokhsareh , Barzegari Abolfazl , Teimuri-Mofrad Reza , Kordasht Houman Kholafazad , Hasanzadeh Mohammad , Khoubnasabjafari Maryam , Jouyban-Gharamaleki Vahid , Rad Abbas Afrasiabi , Shadjou Nasrin , Rashidi Mohammad-Reza , Afshar Mogaddam Mohammad Reza , Jouyban Abolghasem
Published: September 2022

For the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5′-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT CAC TAG TGA ATT-3′ displayed a high binding affinity to RIV and had an efficient ability to discriminate RIV from similar molecular structures. A novel label-free electrochemical aptasensor was designed and fabricated through the conjugation of a thiolated aptamer with Au nanoparticles (Au-NPs). Then, the aptasensor was successfully applied for the quantitative determination of RIV in human plasma and exhaled breath condensate (EBC) samples with limits of detection (LODs) of 14.08 and 6.03 nM, respectively. These valuable results provide ample evidence of the green electrogeneration of AuNPs on the surface of electrodes and their interaction with loaded aptamers (based on Au-S binding) towards the sensitive and selective monitoring of RIV in human plasma and EBC samples. This bio-assay is an alternative approach for the clinical analysis of RIV and has improved specificity and affinity. As far as we know, this is the first time that an electrochemical aptasensor has been verified for the recognition of RIV and that allows for the easy, fast, and precise screening of RIV in biological samples.
Strongly Improving the Sensitivity of Phosphorescence-Based Optical Oxygen Sensors by Exploiting Nano-Porous Substrates
Authors: Liu Chih-Yi , Sadhu Annada Sankar , Karmakar Riya , Chu Cheng-Shane , Lin Yi-Nan , Chang Shih-Hsin , Dalapati Goutam Kumar , Biring Sajal
Published: September 2022

Sensitivity is one of the crucial factors in determining the quality of a fluorescence/phosphorescence-based gas sensor, and is estimated from the measurement of responses (I0/I, where I0 and I refer to the measured optical intensity of a sensor in absence and presence of analyte molecules) at various concentrations of analytes. In this work, we demonstrate phosphorescence-based optical oxygen sensors fabricated on highly porous anodic aluminum oxide (AAO) membranes showing dramatically high response. These sensors exploit the enormous surface area of the AAO to facilitate the effective interaction between the sensing molecules and the analytes. We spin-coat an AAO membrane (200 nm pore diameter) with a platinum-based oxygen sensing porphyrin dye, platinum(II) meso-tetrakis (pentafluorophenyl) porphyrin (PtTFPP), to fabricate a sensor exhibiting I0/I ~400 at 100% oxygen atmosphere. To address the generality of the AAO membrane, we fabricate a separate sensor with another porphyrin dye, platinum octaethylporphyrin (PtOEP), which exhibits an even higher I0/I of ~500. Both of these sensors offer the highest responses as an optical oxygen sensor hitherto reported. SEM and EDS analysis are performed to realize the effect of the increased surface area of the AAO membrane on the enhanced sensitivity.
Identification and Characterization of Elevated Expression of Transferrin and Its Receptor TfR1 in Mouse Models of Depression
Authors: Chang Xin , Ma Mengxin , Chen Liping , Song Zhihong , Zhao Zhe , Shen Wei , Jiang Huihui , Wu Yan , Fan Ming , Wu Haitao
Published: September 2022

Depression has become one of the severe mental disorders threatening global human health. In this study, we first used the proteomics approach to obtain the differentially expressed proteins in the liver between naive control and chronic social defeat stress (CSDS) induced depressed mice. We have identified the upregulation of iron binding protein transferrin (TF) in the liver, the peripheral blood, and the brain in CSDS-exposed mice. Furthermore, bioinformatics analysis of the Gene Expression Omnibus (GEO) database from various mouse models of depression revealed the significantly upregulated transcripts of TF and its receptor TfR1 in multiple brain regions in depressed mice. We also used the recombinant TF administration via the tail vein to detect its permeability through the blood-brain barrier (BBB). We demonstrated the permeability of peripheral TF into the brain through the BBB. Together, these results identified the elevated expression of TF and its receptor TfR1 in both peripheral liver and the central brain in CSDS-induced depressed mice, and peripheral administration of TF can be transported into the brain through the BBB. Therefore, our data provide a compelling information for understanding the potential role and mechanisms of the cross-talk between the liver and the brain in stress-induced depression.
Photocatalytic Reduction of Hexavalent Chromium Using Cu3.21Bi4.79S9/g-C3N4 Nanocomposite
Authors: Ajiboye Timothy O. , Oyewo Opeyemi A. , Marzouki Riadh , Onwudiwe Damian C.
Published: September 2022

The photocatalytic reduction of hexavalent chromium, Cr(VI), to the trivalent species, Cr(III), has continued to inspire the synthesis of novel photocatalysts that are capable of achieving the task of converting Cr(VI) to the less toxic and more useful species. In this study, a novel functionalized graphitic carbon nitride (Cu3.21Bi4.79S9/gC3N4) was synthesized and characterized by using X-ray diffraction (XRD), thermogravimetry analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and scanning electron microscope (SEM). The composite was used for the photocatalytic reduction of hexavalent chromium, Cr(VI), under visible light irradiation. A 92.77% efficiency of the reduction was achieved at pH 2, using about 10 mg of the photocatalyst and 10 mg/L of the Cr(VI) solution. A pseudo-first-order kinetic study indicated 0.0076 min−1, 0.0286 min−1, and 0.0393 min−1 rate constants for the nanoparticles, pristine gC3N4, and the nanocomposite, respectively. This indicated an enhancement in the rate of reduction by the functionalized gC3N4 by 1.37- and 5.17-fold compared to the pristine gC3N4 and Cu3.21Bi4.79S9, respectively. A study of how the presence of other contaminants including dye (bisphenol A) and heavy-metal ions (Ag(I) and Pb(II)) in the system affects the photocatalytic process showed a reduction in the rate from 0.0393 min−1 to 0.0019 min−1 and 0.0039 min−1, respectively. Finally, the radical scavenging experiments showed that the main active species for the photocatalytic reduction of Cr(VI) are electrons (e−), hydroxyl radicals (·OH−), and superoxide (·O2−). This study shows the potential of functionalized gC3N4 as sustainable materials in the removal of hexavalent Cr from an aqueous solution.
Controlled Synthesis of Chromium-Oxide-Based Protective Layers on Pt: Influence of Layer Thickness on Selectivity
Authors: Worsley Myles , Smulders Vera , Mei Bastian
Published: September 2022

Chromium-oxyhydroxide (CrxOyHz)-based thin films have previously been shown in photocatalysis and industrial chlorate production to prevent unwanted reduction reactions to occur, thereby enhancing the selectivity for hydrogen evolution and thus the overall process efficiency. Here, a highly reproducible synthesis protocol was developed to allow for the electrodeposition of CrxOyHz-based thin films with controlled thickness in the range of the sub-monolayer up to (>4) multilayer coverage. Electrodeposited CrxOyHz coatings were electrochemically characterized using voltammetry and stripping experiments, allowing thickness-dependent film selectivity to be deduced in detail. The results are discussed in terms of mass transport properties and structure of the electrodeposited chromium oxyhydroxide films. It is shown that the permeation of diatomic probe molecules, such as O2 and CO, was significantly reduced by films as thin as four monolayers. Importantly, it is shown that the prepared thin film coatings enabled prolonged hydrogen oxidation in the presence of CO (up to 5 vol.%), demonstrating the benefits of thin-film-protected electrocatalysts. In general, this study provides insight into the synthesis and use of thin-film-protected electrodes leading to improvements in (electro)catalyst selectivity and durability.
Chloride-Enhanced Removal of Ammonia Nitrogen and Organic Matter from Landfill Leachate by a Microwave/Peroxymonosulfate System
Authors: Feng Ke , Li Qibin
Published: September 2022

Landfill leachate contains not only high concentrations of refractory organic matter and ammonia nitrogen, but also high concentrations of chloride ions (Cl−). The modification of reactive species of the peroxymonosulfate (PMS) oxidation system by Cl− and its priority sequence for the removal of NH4+-N and organic matter from landfill leachate remain unclear. This study investigated the removal characteristics of NH4+-N and organic matter in the microwave (MW)/PMS system with high Cl− content. The results show that increasing Cl− concentration significantly improves the production of hypochlorous acid (HOCl) in the MW/PMS system under acidic conditions, and that the thermal and non-thermal effects of MW irradiation have an important influence on the HOCl produced by PMS activation. The maximum cumulative concentration of HOCl was 748.24 μM after a reaction time of 2 min. The formation paths of HOCl are (i) SO4•− formed by the MW/PMS system interacting with Cl− and HO•, and (ii) the nucleophilic addition reaction of PMS and Cl−. Moreover, the high concentration of HOCl produced by the system can not only remove NH4+-N in situ, but also interact with PMS to continuously generate Cl• as an oxidant to participate in the reaction with pollutants (e.g., NH4+-N and organic matter). Common aqueous substances (e.g., CO32−, HCO3−, NO3−, and humic acid) in landfill leachate will compete with NH4+-N for reactive species in the system, and will thereby inhibit its removal to a certain extent. It was found that when NH4+-N and leachate DOM co-exist in landfill leachates, they would compete for reactive species, and that humic acid-like matter was preferentially removed, leading to the retention of fulvic acid-like matter. It is hoped that this study will provide theoretical support for the design and optimization of methods for removing NH4+-N and organic matter from landfill leachate with high chloride ion content.
Highly Active Rutile TiO2 for Photocatalysis under Violet Light Irradiation at 405 nm
Authors: Amano Fumiaki , Yamamoto Akira , Kumagai Jun
Published: September 2022

Anatase TiO2 is a widely investigated photocatalyst; however, it can only work under ultraviolet (UV) light with wavelengths less than 390 nm (band gap 3.2 eV). Rutile TiO2 can absorb visible light at wavelengths less than 410 nm (band gap 3.0 eV); however, its photocatalytic activity is not high. Herein, we activated rutile TiO2, which was prepared from Evonik TiO2 P 25 through calcination at 800 °C using hydrogen reduction treatment at 700 °C. The photocatalytic activity of the hydrogen-treated TiO2 was as high as P 25 under UV irradiation at 380 nm, which was significantly higher than P 25 under violet light irradiation at 405 nm for the oxidative decomposition of acetic acid in water. Electron spin resonance studies indicate that charge separation is enhanced in reduced TiO2, and their oxygen reduction pathways differ between anatase and rutile. The formation of H2O2 was observed on rutile TiO2; however, it was consumed during photocatalysis to accelerate acetic acid decomposition.
Molecular Regulators of Embryonic Diapause and Cancer Diapause-like State
Authors: Hussein Abdiasis M. , Balachandar Nanditaa , Mathieu Julie , Ruohola-Baker Hannele
Published: September 2022

Embryonic diapause is an enigmatic state of dormancy that interrupts the normally tight connection between developmental stages and time. This reproductive strategy and state of suspended development occurs in mice, bears, roe deer, and over 130 other mammals and favors the survival of newborns. Diapause arrests the embryo at the blastocyst stage, delaying the post-implantation development of the embryo. This months-long quiescence is reversible, in contrast to senescence that occurs in aging stem cells. Recent studies have revealed critical regulators of diapause. These findings are important since defects in the diapause state can cause a lack of regeneration and control of normal growth. Controlling this state may also have therapeutic applications since recent findings suggest that radiation and chemotherapy may lead some cancer cells to a protective diapause-like, reversible state. Interestingly, recent studies have shown the metabolic regulation of epigenetic modifications and the role of microRNAs in embryonic diapause. In this review, we discuss the molecular mechanism of diapause induction.
PPARδ Agonist Promotes Type II Cartilage Formation in a Rabbit Osteochondral Defect Model
Authors: Song Ju-Yong , Park Jae-Suh , Kim Joo-Hwan , Wang Joon-Ho , Heck Holly C. , Heck Bruce E. , Kim Dong-Hyun , Yoo Keon-Hee
Published: September 2022

Osteoarthritis (OA) is a chronic degenerative joint disease accompanied by an inflammatory milieu that results in painful joints. The pathogenesis of OA is multifactorial, with genetic predisposition, environmental factors, and traumatic injury resulting in the direct or indirect loss of cartilage. The articular cartilage can also be damaged by direct focal traumatic injury. Articular cartilage provides a smooth, deformable bearing surface with a low coefficient of friction, increased contact area, and reduced contact stress. Articular type II hyaline cartilage lines the synovial joints and, when injured, has a limited ability for repair, except for the most superficial layers via diffusion from the synovial fluid, secondary to no blood supply, a complex structure, and a low metabolic rate. Restoring the articular surface can relieve pain and restore function. Although many strategies have been developed to regenerate type II collagen based on the extent of the lesion, surgical treatments are still evolving. The peroxisome proliferator-activated receptor delta (PPARδ) agonist and collagen treatment of mesenchymal stem cells (MSCs) enhance the chondrogenic capacity in vitro. We present a novel technique for cartilage restoration in a rabbit cartilage osteochondral defect model using a PPARδ agonist (GW0742)-infused 3D collagen scaffold to induce type II cartilage from MSCs.
Long Noncoding RNAs and Circular RNAs Regulate AKT and Its Effectors to Control Cell Functions of Cancer Cells
Authors: Tang Jen-Yang , Chuang Ya-Ting , Shiau Jun-Ping , Yang Kun-Han , Chang Fang-Rong , Hou Ming-Feng , Farooqi Ammad Ahmad , Chang Hsueh-Wei
Published: September 2022

AKT serine-threonine kinase (AKT) and its effectors are essential for maintaining cell proliferation, apoptosis, autophagy, endoplasmic reticulum (ER) stress, mitochondrial morphogenesis (fission/fusion), ferroptosis, necroptosis, DNA damage response (damage and repair), senescence, and migration of cancer cells. Several lncRNAs and circRNAs also regulate the expression of these functions by numerous pathways. However, the impact on cell functions by lncRNAs and circRNAs regulating AKT and its effectors is poorly understood. This review provides comprehensive information about the relationship of lncRNAs and circRNAs with AKT on the cell functions of cancer cells. the roles of several lncRNAs and circRNAs acting on AKT effectors, such as FOXO, mTORC1/2, S6K1/2, 4EBP1, SREBP, and HIF are explored. To further validate the relationship between AKT, AKT effectors, lncRNAs, and circRNAs, more predicted AKT- and AKT effector-targeting lncRNAs and circRNAs were retrieved from the LncTarD and circBase databases. Consistently, using an in-depth literature survey, these AKT- and AKT effector-targeting database lncRNAs and circRNAs were related to cell functions. Therefore, some lncRNAs and circRNAs can regulate several cell functions through modulating AKT and AKT effectors. This review provides insights into a comprehensive network of AKT and AKT effectors connecting to lncRNAs and circRNAs in the regulation of cancer cell functions.
PGC-1α4 Interacts with REST to Upregulate Neuronal Genes and Augment Energy Consumption in Developing Cardiomyocytes
Authors: Tuomainen Tomi , Naumenko Nikolay , Mutikainen Maija , Shakirzyanova Anastasia , Sczelecki Sarah , Estall Jennifer L. , Ruas Jorge L. , Tavi Pasi
Published: September 2022

Transcriptional coactivator PGC-1α is a main regulator of cardiac energy metabolism. In addition to canonical PGC-1α1, other PGC-1α isoforms have been found to exert specific biological functions in a variety of tissues. We investigated the expression patterns and the biological effects of the non-canonical isoforms in the heart. We used RNA sequencing data to identify the expression patterns of PGC-1α isoforms in the heart. To evaluate the biological effects of the alternative isoform expression, we generated a transgenic mouse with cardiac-specific overexpression of PGC-1α4 and analysed the cardiac phenotype with a wide spectrum of physiological and biophysical tools. Our results show that non-canonical isoforms are expressed in the heart, and that the main variant PGC-1α4 is induced by β-adrenergic signalling in adult cardiomyocytes. Cardiomyocyte specific PGC-1α4 overexpression in mice relieves the RE1-Silencing Transcription factor (REST)-mediated suppression of neuronal genes during foetal heart development. The resulting de-repression of REST target genes induces a cardiac phenotype with increased cellular energy consumption, resulting in postnatal dilated cardiomyopathy. These results propose a new concept for actions of the PGC-1α protein family where activation of the Pgc-1α gene, through its isoforms, induces a phenotype with concurrent supply and demand for cellular energy. These data highlight the biological roles of the different PGC-1α isoforms, which should be considered when future therapies are developed.
Association of Type 2 Deiodinase Thr92Ala Polymorphism with Pediatric Obesity in Japanese Children: A Case-Control Study
Authors: Ota Takeshi , Mori Jun , Kawabe Yasuhiro , Morimoto Hidechika , Fukuhara Shota , Kodo Kazuki , Sugimoto Satoru , Kosaka Kitaro , Nakajima Hisakazu , Hosoi Hajime
Published: September 2022

Genetic factors play critical roles in the onset and progression of obesity. Brown adipose tissue (BAT) activity is also critical for adiposity. The objective of this study was to evaluate the prevalence and effects of BAT gene polymorphisms in pediatric obesity. This case-control study included 270 non-obese and 86 obese children. All participants underwent genotyping for type 2 deiodinase (DIO2) Thr92Ala (rs225014). The prevalence of the homozygous Ala/Ala allele of the DIO2 gene in the obese group was 15.1% versus 6.3% in the non-obese group, resulting in an odds ratio (OR) of 3.393 (p = 0.003). The results of this study indicate that the homozygous Ala/Ala allele of the DIO2 gene is associated with an increased risk of pediatric obesity and suggest that pediatric obesity might be suitable for assessing the association with gene polymorphisms related to BAT, especially DIO2 Thr92Ala.
Oxidative Corrosion Mechanism of Ti2AlNb-Based Alloys during Alternate High Temperature-Salt Spray Exposure
Authors: Chen Wei , Huang Lei , Liu Yaoyao , Zhao Yanfei , Wang Zhe , Xie Zhiwen
Published: September 2022

This study investigates the corrosion damage mechanisms of Ti2AlNb-based alloys under high temperature, salt spray and coupled high temperature-salt spray conditions. This alloy was analysed in detail from macroscopic to microscopic by means of microscale detection (XRD, SEM and EDS). The results indicated that Ti2AlNb-based alloy surface oxide layer is dense and complete, and the thickness is only 3 µm after oxidation at 650 °C for 400 h. Compared to the original sample, the production of the passivation film resulted in almost no damage to Ti2AlNb-based alloy after 50 cycles of salt spray testing at room temperature. The tests showed that Ti2AlNb alloy shows good erosion resistance at 650 °C and in salt spray. However, this alloy had an oxide layer thickness of up to 30 µm and obvious corrosion pits on the surface after 50 cycles of corrosion under alternating high temperature-salt spray conditions. The Cl2 produced by the mixed salt eutectic reaction acted as a catalytic carrier to accelerate the volatilisation of the chloride inside the oxide layer and the re-oxidation of the substrate. In addition, the growth of unprotected corrosion products (Na2TiO3, NaNbO3 and AlNbO4) altered the internal structure of the oxide layer, destroying the surface densification and causing severe damage to the alloy surface.
Strength and Moisture-Related Properties of Filter Paper Coated with Nanocellulose
Authors: Mazela Bartłomiej , Tomkowiak Karolina , Jones Dennis
Published: September 2022

The aim of this study was to assess selected properties of coatings incorporating nanocellulose, with the potential of being applied as a surface modification for cellulosic and lignocellulosic materials, particularly for applications within biodegradable packaging. Cellulose nanocrystal (CNC) and cellulose nanofibril (CNF) coatings were produced and applied on both sides of pure cellulose samples in the form of filter paper with a Mayer bar. Selected organosilicon compounds, albumin, and the TEMPO reagent were used as additional modifying substances. Coating parameters were determined, such as water contact angle, water absorption, hygroscopicity, and tensile strength. The presence of the coatings resulted in a significant increase in water vapor absorption by the substrate. Nanocellulose coatings proved to be sensitive to the water vapor and showed no barrier properties against it. However, the samples coated with nanocellulose had a noticeably lower tendency to absorb liquid water. The samples coated with modifying substances had a contact angle of ≥90°, proving that such coatings were an additional barrier to the penetration of liquid water. In the case of cellulose material coated with nanocellulose (i.e., without the addition of silane), there was no barrier effect established, thereby allowing full wetting of the substrate. The nanocellulose coatings increased the tensile strength of the samples. This increase was observed for all tested coating variants. The results obtained offer several potential routes to the manufacture of more environmentally friendly coatings and packaging materials.
Efficient, Lightweight Cyber Intrusion Detection System for IoT Ecosystems Using MI2G Algorithm
Authors: Kaushik Sunil , Bhardwaj Akashdeep , Alomari Abdullah , Bharany Salil , Alsirhani Amjad , Mujib Alshahrani Mohammed
Published: September 2022

The increase in internet connectivity has led to an increased usage of the Internet of Things (IoT) and devices on the internet. These IoT devices are becoming the backbone of Industry 4.0. The dependence on IoT devices has made them vulnerable to cyber-attacks. IoT devices are often deployed in harsh conditions, challenged with less computational costs, and starved with energy. All these limitations make it tough to deploy accurate intrusion detection systems (IDSs) in IoT devices and make the critical IoT ecosystem more susceptible to cyber-attacks. A new lightweight IDS and a novel feature selection algorithm are introduced in this paper to overcome the challenges of computational cost and accuracy. The proposed algorithm is based on the Information Theory models to select the feature with high statistical dependence and entropy reduction in the dataset. This feature selection algorithm also showed an increase in performance parameters and a reduction in training time of 27–63% with different classifiers. The proposed IDS with the algorithm showed accuracy, Precision, Recall, and F1-Score of more than 99% when tested with the CICIDS2018 dataset. The proposed IDS is competitive in accuracy, Precision, Recall, and training time compared to the latest published research. The proposed IDS showed consistent performance on the UNSWNB15 dataset.
Effect of rGO wt.% on the Preparation of rGO/CuO Nanocomposites at Different Test Periods and Temperatures
Authors: Alanazi Abdullah K. , Abo-Dief Hala M. , Alothman Zaid A. , Mohamed Ashraf T. , Pramanik Tanay , Fallata Ahmed M.
Published: September 2022

In this study, we synthesized a reduced form of graphene oxide/copper oxide (rGO/CuO) nanocompounds produced at rGO wt. of 0.125%, 0.25%, 0.5% and 1%. The crystallinity indexes for rGO and rGO/CuO increased, and that for CuO decreased as the test temperatures increases, while the crystallinity indexes of rGO, CuO and rGO/CuO decreases with test periods increment. Measurement by dynamic light scattering reported average crystallite sizes of 0.7, 8.8, 25.4, 38.5 nm for 0.125 wt.% rGO/CuO, 0.25 wt.% rGO/CuO, 0.50 wt.% rGO/CuO and 1.0 wt.% rGO/CuO respectively. The electrochemical properties of the nanocomposites were checked. The rGO/CuO XRD peaks were 18.114320 Å, 225.1856 Å, 321.41740 Å, and 365.98290 Å, with 11.051640%, 0.461075%, 0.280083%, and 0.174259% for 2ϴ of 22.2031°, 43.5865°, 50.7050°, and 74.3729°, respectively. FTIR spectroscopy identified the existence of vibrational frequencies with pseudo-capacitance at 458 cm−1 which confirmed the presence of rGO-CuO nanoparticles. The voltammetry of rGO-CuO indicated the increment of electrochemical activity, large capacitance, and conduction in the reduced rGO/CuO composite. For rGO wt. of 0.125%, 0.25%, 0.5%, and 1.0%, the rGO/CuO composite specific capacitance was 561 F/g, 582 F/g, 597 F/g, and 611 F/g, respectively, which indicated good electrochemical performance.
Autoantibodies to Oxidatively Modified Peptide: Potential Clinical Application in Coronary Artery Disease
Authors: Tsai I-Jung , Shen Wen-Chi , Wu Jia-Zhen , Chang Yu-Sheng , Lin Ching-Yu
Published: September 2022

Coronary artery disease (CAD) is a global health issue. Lipid peroxidation produces various by-products that associate with CAD, such as 4-hydroxynonenal (HNE) and malondialdehyde (MDA). The autoantibodies against HNE and MDA-modified peptides may be useful in the diagnosis of CAD. This study included 41 healthy controls (HCs) and 159 CAD patients with stenosis rates of <30%, 30–70%, and >70%. The plasma level of autoantibodies against four different unmodified and HNE-modified peptides were measured in this study, including CFAH1211–1230, HPT78–108, IGKC2–19, and THRB328–345. Furthermore, feature ranking, feature selection, and machine learning models have been utilized to exploit the diagnostic performance. Also, we combined autoantibodies against MDA and HNE-modified peptides to improve the models’ performance. The eXtreme Gradient Boosting (XGBoost) model received a sensitivity of 78.6% and a specificity of 90.4%. Our study demonstrated the combination of autoantibodies against oxidative modification may improve the model performance.
Early Detection of Obstacle to Optimize the Robot Path Planning
Authors: Sharma Kaushlendra , Swarup Chetan , Pandey Saroj Kumar , Kumar Ankit , Doriya Rajesh , Singh Kamred Udham , Singh Teekam
Published: September 2022

Robot path planning is one of the core issues in robotics and its application. Optimizing the route discovery becomes more important while dealing with the robot-based application. This paper proposes the concept of early detection of the obstacle present in the workspace of the robots. To early detect the obstacle, this paper proposes the concept of a snake algorithm along with the traditional path planning algorithms. The contour detection part is merged with the different path planning algorithms to optimize the robot traversing and benefit it in producing good results. Obstacle-free optimized path is one of the core requirements for robots in any application. With the help of path planning algorithms, robots are enabled to derive those paths in a specific environment. The presence of an obstacle makes it difficult for any path planning algorithms to derive a smooth path. The purpose of using the snake algorithm is to detect an obstacle early. This method not only perceives the obstacle but also catches out the complete boundary of the obstacle, it, thus, provides the details of obstacle coordinates to the path planning algorithm. Conceiving the complete periphery of obstacles can have multiple advantages in many application areas. A*, PRM, RRT, and RRT Smooth algorithms are considered along with the snake algorithm to validate our work in three different experimental scenarios: Maze, Random Obstacles, and Dense case. Path length, Time-taken, and Move count are parameters taken to observe the results. The result obtained using the snake algorithm with four path planning algorithms is analyzed and compared in detail with the core A*, PRM, RRT, and RRTS. Finally, the result obtained using the proposed methodology gives some encouraging results and also predicts the exploration of the robot’s path planning for more applications and fields.
Introduction to the Special Issue “Mathematics Education: At Home and in the Classroom”
Authors: Sonnenschein Susan , Stites Michele L.
Published: September 2022

Children’s mathematics achievements differ based on a variety of factors, including country of origin and cultural or economic background [...]
In-Depth Assessment of Cross-Passage Critical Velocity for Smoke Control in Large-Scale Railway Tunnel Fires
Authors: Chen Timothy Bo Yuan , Yuen Anthony Chun Yin , De Cachinho Cordeiro Ivan Miguel , Liu Hengrui , Cao Ruifeng , Ellison Amy , Yeoh Guan Heng
Published: September 2022

Demand for underground railways has rapidly increased due to accelerated urbanisation and population growth. This has elevated the importance of tunnel designs with adequate fire safety and protection measures. However, due to intricate modern rail tunnel designs, prescriptive codes are often difficult to implement and lead to over-conservative design. In this study, the current state of tunnel fire analysis was reviewed with a focus on Australia. A large-eddy simulation (LES)-based fire model was applied to investigate the temperature and smoke dispersion from a 2 MW metro tunnel fire case scenario to the cross-passage. A total of 28 cases with various cross-passage ventilation settings were examined, including longitudinal tunnel velocity, cross-passage velocity, train location relative to the cross-passage and fire location. The modelling showed that a 0.84 m/s critical velocity was sufficient for smoke control in the cross-passage. Furthermore, two empirical methods for cross-passage critical velocity were performed, which showed utilisation of the Froude number produced a less conservative critical velocity (0.610 m/s) compared to the dimensionless method (0.734 m/s). Nevertheless, both numerical and empirical results were significantly lower than the standard 1.0 m/s minimum flow rate for smoke control (AS1668.1). The results provide preliminary evidence towards the need for revision of current tunnel fire standards and response protocols.
Neutron Star Radii, Deformabilities, and Moments of Inertia from Experimental and Ab Initio Theory Constraints of the 208Pb Neutron Skin Thickness
Authors: Lim Yeunhwan , Holt Jeremy W.
Published: September 2022

Recent experimental and ab initio theory investigations of the 208Pb neutron skin thickness have the potential to inform the neutron star equation of state. In particular, the strong correlation between the 208Pb neutron skin thickness and the pressure of neutron matter at normal nuclear densities leads to modified predictions for the radii, tidal deformabilities, and moments of inertia of typical 1.4M⊙ neutron stars. In the present work, we study the relative impact of these recent analyses of the 208Pb neutron skin thickness on bulk properties of neutron stars within a Bayesian statistical analysis. Two models for the equation of state prior are employed in order to highlight the role of the highly uncertain high-density equation of state. From our combined Bayesian analysis of nuclear theory, nuclear experiment, and observational constraints on the dense matter equation of state, we find at the 90% credibility level R1.4=12.36−0.73+0.38 km for the radius of a 1.4M⊙ neutron star, R2.0=11.96−0.71+0.94 km for the radius of a 2.0M⊙ neutron star, Λ1.4=440−144+103 for the tidal deformability of a 1.4M⊙ neutron star, and I1.338=1.425−0.146+0.074×1045gcm2 for the moment of inertia of PSR J0737-3039A whose mass is 1.338M⊙.
Evaluation of Geriatric Sarcopenia and Nutrition in the Case of Cachexia before Exitus: An Observational Study for Health Professionals
Authors: Moga Titus David , Moga Ioana , Sabău Monica , Venter Alina Cristiana , Romanescu Dana , Bimbo-Szuhai Erika , Costas Lavinia Mihaela , Huniadi Anca , Rahota Diana Maria
Published: September 2022

It is important to assess the physical and nutritional status of the body using a bioelectrical impedance analyzer (BIA) in patients with cachexia; however, the correlation between cachexia and nutritional evaluations remains unclear. The objective of this study is to follow the effects of diet therapy in patients with cachexia/sarcopenia, using parameters measured by BIA, clinical parameters, and other nutrition-related assessments in patients with osteoporosis. This study aims to clarify the correlation between BIA-measured parameters, clinical parameters, and other nutrition-related assessments. Methods: Measurements of body composition, a clinical assessment of the sarcopenia/cachexia, and nutritional goal setting/a nutrition care process were performed. Results: The number of subjects was 200, of which 15 people (7.5%) were diagnosed with sarcopenia/cachexia. Univariate analyses showed that participants with a high body-fat mass tend to develop sarcopenic obesity (p = 0.029), those who lost a significant and progressive amount of muscle mass tend to develop sarcopenia (p = 0.001), as well as those with malnutrition (p < 0.001). The regression study shows not only the correlation but also the cause of the correlation, as is the case with obesity. As obesity increases, so does the sarcopenic index (this can explain sarcopenic obesity), and as fat mass decreases it leads to muscle mass loss, increasing the risk of cachexia with age. Conclusions: There was an improvement, but statistically insignificant, in cachexia and the nutritional objectives (p > 0.05); at the same time, correlations were established between the independent parameters (sex, age) and malnutrition parameters (hemoglobin and amylase) with the parameters of the research.