bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Treating Hatching Eggs with Curcumin after Exposure to Thermal Stress on Embryonic Development, Hatchability, Physiological Body Reactions, and Hormonal Profiles of Dokki-4 Chickens
Authors: Abuoghaba Ahmed Abdel-Kareem , Ragab Mona A. , Shazly Soheir A. , Kokoszyński Dariusz , Saleh Mohamed
Published: November 2021

This study evaluated the impact of incubation temperature and spraying hatching eggs with curcumin during the early embryogenesis phase on chick embryo developments, hatchability, physiological body reactions, and hormonal profiles of Dokki 4 chickens. A total of 720 fertile eggs were equally distributed into two groups. In the first group, the eggs were incubated at normal incubation temperature/NIT (37.8 °C and 55–60% RH) for up to 19 days of incubation, whereas those in the second group were incubated in the same conditions except from 6 to 8 day, in which they were daily exposed to chronic incubation temperature/CIT (39.0 °C) for 3 h. Each group was classified into four curcumin treatment doses; the 1st treatment (control) was sprayed with distilled water, while the 2nd, 3rd, and 4th treatments were sprayed with 250, 500, and 1000 mg curcumin/liter distilled water. The results indicated that the lowest hatchability of fertile eggs (%) was obtained in the CIT group (p = 0.02), whereas the highest body surface temperature/BST compared in the NIT group (p = 0.01). Regarding curcumin treatments, the percentages of heart, gizzard, spleen, and T3 hormone levels in the treated group were significantly increased, while the H/L ratio was significantly reduced (p = 0.001) compared with the control. At 8 weeks of age, the testes and ovary percentages in treated groups were significantly (p = 0.05) increased compared with the control. In conclusion, exposure of hatching eggs to high thermal stress (39 °C) during the incubation phase had deleterious effects on chick performance and T3 hormone level. Moreover, spraying hatching eggs had beneficial impacts on growth, reproductive organs, T3 hormone level, and reducing H/L ratio.
Lamb Meat Quality and Carcass Evaluation of Five Autochthonous Sheep Breeds: Towards Biodiversity Protection
Authors: Ciliberti Maria Giovanna , Santillo Antonella , Marino Rosaria , Ciani Elena , Caroprese Mariangela , Rillo Luigina , Matassino Donato , Sevi Agostino , Albenzio Marzia
Published: November 2021

In the present study, the evaluation of the carcasses and meat quality, in terms of chemical composition and fatty acid profile, of lambs from five autochthonous sheep breeds (Altamurana, Bagnolese, Gentile di Puglia, Laticauda, and Leccese) reared in continental Southern Italy, were studied. All the carcasses were evaluated according to the EU Mediterranean classification system for carcasses weighing less than 13 kg. Meat chemical composition and fatty acids profile were assessed on both loin and leg commercial cuts. Fatty acid composition of loin resulted in differences among breeds, displaying lower values of saturated fatty acid in Altamurana, Bagnolese, and Leccese breeds and the highest content of polyunsaturated fatty acid in the Altamurana breed. Principal component analysis grouped lamb according to fatty acid content and to conjugated linoleic acid (CLA), omega n-3 and n-6 fatty acids; thus, Altamurana, Bagnolese, and Leccese breeds are characterized by the highest values of CLA content. Our data demonstrated that lamb meat from autochthonous breed has good carcass quality and the content of CLA, n-3, and n-6 was valuable for human consumption; therefore, the valorisation of local meat quality can help to avoid the extinction of the autochthonous breed offering to the market and consumer’s high nutritive products.
Impacts from Wildfires on Livestock Health and Production: Producer Perspectives
Authors: O’Hara Kathleen C. , Ranches Juliana , Roche Leslie M. , Schohr Tracy Kay , Busch Roselle C. , Maier Gabriele U.
Published: November 2021

Wildfires are increasing in frequency and severity across the Western United States. However, there is limited information available on the impacts these fires are having on the livelihood of livestock producers and their animals. This work presents the results of a survey evaluating the direct and indirect impacts of the 2020 wildfire season on beef cattle, dairy cattle, sheep, and goat, producers in California, Oregon, and Nevada. Seventy completed surveys were collected between May and July 2021. While dairy producers reported no direct impacts from the fires, beef, sheep, and goat producers were impacted by evacuations and pasture lost to fires. Only beef producers reported losses due to burns and burn-associated deaths or euthanasia. Dairy, beef, sheep, and goat producers observed reduced conception, poor weight gain, and drops in milk production. All but dairy producers also observed pneumonia. Lower birthweights, increased abortion rates, and unexplained deaths were reported in beef cattle, sheep, and goats. This work documents the wide-ranging impacts of wildfires on livestock producers and highlights the need for additional work defining the health impacts of fire and smoke exposure in livestock, as well as the policy changes needed to support producers experiencing direct and indirect losses.
Correlation of Genomic and Pedigree Inbreeding Coefficients in Small Cattle Populations
Authors: Cortes-Hernández José , García-Ruiz Adriana , Vásquez-Peláez Carlos Gustavo , Ruiz-Lopez Felipe de Jesus
Published: November 2021

This study aimed to identify inbreeding coefficient (F) estimators useful for improvement programs in a small Holstein population through the evaluation of different methodologies in the Mexican Holstein population. F was estimated as follows: (a) from pedigree information (Fped); (b) through runs of homozygosity (Froh); (c) from the number of observed and expected homozygotic SNP in the individuals (Fgeno); (d) through the genomic relationship matrix (Fmg). The study included information from 4277 animals with pedigree records and 100,806 SNP. The average and standard deviation values of F were 3.11 ± 2.30 for Fped, −0.02 ± 3.55 for Fgeno, 2.77 ± 0.71 for Froh and 3.03 ± 3.05 for Fmg. The correlations between coefficients varied from 0.30 between Fped and Froh, to 0.96 between Fgeno and Fmg. Differences in the level of inbreeding among the parent’s country of origin were found regardless of the method used. The correlations among genomic inbreeding coefficients were high; however, they were low with Fped, so further research on this topic is required.
Application of Probabilistic Neural Networks Using High-Frequency Components’ Differential Current for Transformer Protection Schemes to Discriminate between External Faults and Internal Winding Faults in Power Transformers
Authors: Chiradeja Pathomthat , Pothisarn Chaichan , Phannil Nattanon , Ananwattananporn Santipont , Leelajindakrairerk Monthon , Ngaopitakkul Atthapol , Thongsuk Surakit , Pornpojratanakul Vinai , Bunjongjit Sulee , Yoomak Suntiti
Published: November 2021

Internal and external faults in a power transformer are discriminated in this paper using an algorithm based on a combination of a discrete wavelet transform (DWT) and a probabilistic neural network (PNN). DWT decomposes high-frequency fault components using the maximum coefficients of a ¼ cycle DWT as input patterns for the training process in a decision algorithm. A division algorithm between a zero sequence of post-fault differential current waveforms and the differential current coefficient in the ¼ cycle DWT is used to detect the maximum ratio and faults. The simulation system uses various study cases based on Thailand’s electricity transmission and distribution systems. The simulation results demonstrated that the PNN and BPNN are effectively implemented and perform fault detection with satisfactory accuracy. However, the PNN method is most suitable for detecting internal and external faults, and the maximum coefficient algorithm is the most effective in detecting the fault. This study will be useful in differential protection for power transformers.
Dairy Waste and Potential of Small-Scale Biogas Digester for Rural Energy in India
Authors: Pandey Prachi , Pandey Aditya , Yan Long , Wang Dengshan , Pandey Vinay , Meikap Bhim Charan , Huo Jiang , Zhang Ruihong , Pandey Pramod K.
Published: November 2021

In order to reduce emissions of greenhouse gases, related global warming and dependency on fossil fuels, it is crucial to promote the uses of renewable energy, and conversion of biomass and organic waste into energy sources. In many parts of the world, a substantial increase in efforts for the conversion of waste into energy is currently being observed. Specifically, biogas technology has been emphasized for the conversion of animal waste into biomethane/biogas because livestock waste is considered to be a substantial source of ambient greenhouse gases, causing climate change. While biogas technology, an anerobic process to convert livestock waste into biogas, is promoted in both developed and developing countries, this review article is focused on improving our existing understanding of small-scale biogas technology and relevance of this technology in rural environment of India. A thorough review research has been performed to gather the information on livestock population, manure production, and potential of biogas technology in India to provide a wholistic information. A summary of the financial supports facilitated by various agencies, the cost of biogas plants, potential uses, and potential challenges in the dissemination of biogas technology in India has been discussed in this study. We anticipate that the data and interpretation provided here will help in understanding the scope of biogas technology in India and will help in formulating the policies which will support the implementation of biogas technologies in developing countries.
The Connection between Extreme Precipitation Variability over Monsoon Asia and Large-Scale Circulation Patterns
Authors: Khadgarai Sunilkumar , Kumar Vinay , Pradhan Prabodha Kumar
Published: November 2021

Spatial and temporal variability in precipitation has been dramatically changed due to climate variability and climate change over the global domain. Increasing in extreme precipitation events are pronounced in various regions, including monsoon Asia (MA) in recent decades. The present study evaluated precipitation variability in light of intensity, duration, and frequency with several extreme precipitation climate change indices developed by the Expert Team on Climate Change Detection Indices (ETCCDI) over the MA region. This study uses an improved version (APHRO_V1901) of the Asian Precipitation Highly Resolved Observation Data Integration Towards Evaluation of extreme events (APHRODITE-2) gridded rainfall product. Results showed that the spatial variability of the extreme precipitation climate change indices is reflected in the annual mean rainfall distribution in MA. Maximum one-day precipitation (R × 1) and precipitation contributed from extremes (R95) depict a peak in decadal mean rainfall values over topography regions. A significant positive trend in R × 1 (with a slope of 0.3 mm/yr) and precipitation greater than the 95th percentile (R95: with a slope of 0.5 mm/yr) are predominantly observed in decadal trends in regional average extreme precipitation climate change indices over MA. Maritime continental countries exhibit an inclined trend in R10, whereas central Asian arid regions show a decreasing tendency in continuous dry days (CDD). The positive trend in R95 is observed over central India, the monsoon region in China, countries that reside over the equator and some parts of Japan, and the Philippines. When comparing the influence of surface temperature (T) and total column water vapor (TCW) on precipitation climate change indices, TCW seems to be a crucial attributor to climate change indices meridional variability. The mutual correlation analysis depicts that precipitation contributed from extremes (R95) strongly correlates in terms of temporal variability with all extreme precipitation indices. Among various global circulation patterns, the prevalent conditions of sea surface temperature (SST) over the equatorial Pacific Ocean have a significant influence on decadal variability in extreme precipitation climate change indices. R10 and R95 possess a relatively significant correlation (0.86 and 0.91) with the Southern Oscillation Index. The maximum number of consecutive dry days (CDD) shows an increasing trend with a positive phase of the North Atlantic Oscillation Index.
Undiluted Measurement of the Particle Size Distribution of Different Oxygenated Biofuels in a Gasoline-Optimised DISI Engine
Authors: Larsson Tara , Olofsson Ulf , Christiansen Erlandsson Anders
Published: November 2021

The utilisation of internal combustion engines is one of the main causes of particle emissions in urban areas. As the interest for the utilisation of biofuels increases, it is important to understand their effect on particle number emissions. In this paper, the particle size distribution and the particle number emissions from a gasoline-optimised direct-injected spark-ignited (DISI) engine are investigated. The effects of five different biofuel alternatives on these emissions were evaluated and compared to gasoline. The utilisation of the high-resolution, high-temperature ELPI+ enabled undiluted measurements of the particle size distribution down to 6 nm, without extensive cooling of the engine exhaust. Contrary to other studies, the results show that the particle number emissions for the three measured cut-off sizes (23, 10 and 7 nm) increased with the utilisation of oxygenated biofuels. The results indicate that the decreased volatility and energy density of the alcohols has a more significant impact on the particle formation in a DISI engine than the increased oxygen content of these fuels.
Age-Related Intrinsic Functional Connectivity Changes of Locus Coeruleus from Childhood to Older Adults
Authors: Song Inuk , Neal Joshua , Lee Tae-Ho
Published: November 2021

The locus coeruleus is critical for selective information processing by modulating the brain’s connectivity configuration. Increasingly, studies have suggested that LC controls sensory inputs at the sensory gating stage. Furthermore, accumulating evidence has shown that young children and older adults are more prone to distraction and filter out irrelevant information less efficiently, possibly due to the unoptimized LC connectivity. However, the LC connectivity pattern across the life span is not fully examined yet, hampering our ability to understand the relationship between LC development and the distractibility. In this study, we examined the intrinsic network connectivity of the LC using a public fMRI dataset with wide-range age samples. Based on LC-seed functional connectivity maps, we examined the age-related variation in the LC connectivity with a quadratic model. The analyses revealed two connectivity patterns explicitly. The sensory-related brain regions showed a positive quadratic age effect (u-shape), and the frontal regions for the cognitive control showed a negative quadratic age effect (inverted u-shape). Our results imply that such age-related distractibility is possibly due to the impaired sensory gating by the LC and the insufficient top-down controls by the frontal regions. We discuss the underlying neural mechanisms and limitations of our study.
AngIV-Analog Dihexa Rescues Cognitive Impairment and Recovers Memory in the APP/PS1 Mouse via the PI3K/AKT Signaling Pathway
Authors: Sun Xiaojin , Deng Yang , Fu Xinxin , Wang Siyu , Duan Rui , Zhang Yingdong
Published: November 2021

The renin-angiotensin system (RAS) is a paracrine RAS within the central nervous system (CNS) and is closely related to Alzheimer’s disease (AD). The endogenous hexapeptide angiotensin IV (Ang IV), an important component of the brain RAS, was found to rescue cognitive impairment and recover memory in previous studies. In our study, we used different doses of Dihexa, which can be orally administered and cross the BBB in APP/PS1 mice. We found that the amount of AngIV in mouse tissue increased after the administration of Dihexa compared to that in the WT group. Meanwhile, Dihexa restored spatial learning and cognitive functions in the Morris water maze test. Dihexa increased the neuronal cells and the expression of SYP protein in APP/PS1 mice in Nissl staining. Furthermore, Dihexa decreased the activation of astrocytes and microglia, markedly reduced levels of the pro-inflammatory cytokines IL-1β and TNF-α and increased the levels of the anti-inflammatory cytokine IL-10. Dihexa activated the PI3K/AKT signaling pathway, while PI3K inhibitor wortmannin significantly reversed the anti-inflammatory and anti-apoptotic effects of APP/PS1 mice. These findings highlight the brain AngIV/PI3K/AKT axis as a potential target for the treatment of AD.
Prism Adaptation and Optokinetic Stimulation Comparison in the Rehabilitation of Unilateral Spatial Neglect
Authors: Facchin Alessio , Figliano Giusi , Daini Roberta
Published: November 2021

Prism adaptation (PA) is one of the most effective treatments for the rehabilitation of unilateral spatial neglect. Optokinetic stimulation (OKS) has also been demonstrated to be effective in ameliorating symptoms of neglect. The aim of this study is to compare the effectiveness of these two methods in a group of neglect patients using a crossover design. A group of 13 post-acute brain-damaged patients with unilateral spatial neglect, who had never been rehabilitated, were treated using PA and OKS. Each treatment was applied for 10 sessions, twice a day, to all patients with both treatments in crossed order (i.e., PA followed by OKS or vice versa). Neuropsychological assessments were performed: before the first (T1), at the end of the first/beginning of the second (T2) and at the end of the second training sessions (T3), and two weeks after the end of treatment (T4). Both procedures produced a significant improvement in clinical tests at T2, independent of the type of training. The results suggest that either PA or OKS induces a significant amelioration of neglect in right brain-damaged patients, mainly in the first block of treatment. Since no differences between treatments were found, they could be applied in clinical practice, according to the requirements of the individual patient.
Resting-State Networks Associated with Behavioral and Self-Reported Measures of Persecutory Ideation in Psychosis
Authors: Yu Lingyan , Kazinka Rebecca , Pratt Danielle , Kwashie Anita , MacDonald Angus W.
Published: November 2021

Persecutory ideations are self-referential delusions of being the target of malevolence despite a lack of evidence. Wisner et al. (2021) found that reduced connectivity between the left frontoparietal (lFP) network and parts of the orbitofrontal cortex (OFC) correlated with increased persecutory behaviors among psychotic patients performing in an economic social decision-making task that can measure the anticipation of a partner’s spiteful behavior. If this pattern could be observed in the resting state, it would suggest a functional-structural prior predisposing individuals to persecutory ideation. Forty-four patients in the early course of a psychotic disorder provided data for resting-state functional connectivity magnetic resonance imaging across nine brain networks that included the FP network and a similar OFC region. As predicted, we found a significant and negative correlation between the lFP–OFC at rest and the level of suspicious mistrust on the decision-making task using a within-group correlational design. Additionally, self-reported persecutory ideation correlated significantly with the connectivity between the right frontoparietal (rFP) network and the OFC. We extended the previous finding of reduced connectivity between the lFP network and the OFC in psychosis patients to the resting state, and observed a possible hemispheric difference, such that greater rFP–OFC connectivity predicted elevated self-reported persecutory ideation, suggesting potential differences between the lFP and rFP roles in persecutory social interactions.
Anatomically Standardized Detection of MRI Atrophy Patterns in Early-Stage Alzheimer’s Disease
Authors: Lenhart Lukas , Seiler Stephan , Pirpamer Lukas , Goebel Georg , Potrusil Thomas , Wagner Michaela , Dal Bianco Peter , Ransmayr Gerhard , Schmidt Reinhold , Benke Thomas , Scherfler Christoph
Published: November 2021

MRI studies have consistently identified atrophy patterns in Alzheimer’s disease (AD) through a whole-brain voxel-based analysis, but efforts to investigate morphometric profiles using anatomically standardized and automated whole-brain ROI analyses, performed at the individual subject space, are still lacking. In this study we aimed (i) to utilize atlas-derived measurements of cortical thickness and subcortical volumes, including of the hippocampal subfields, to identify atrophy patterns in early-stage AD, and (ii) to compare cognitive profiles at baseline and during a one-year follow-up of those previously identified morphometric AD subtypes to predict disease progression. Through a prospectively recruited multi-center study, conducted at four Austrian sites, 120 patients were included with probable AD, a disease onset beyond 60 years and a clinical dementia rating of ≤1. Morphometric measures of T1-weighted images were obtained using FreeSurfer. A principal component and subsequent cluster analysis identified four morphometric subtypes, including (i) hippocampal predominant (30.8%), (ii) hippocampal-temporo-parietal (29.2%), (iii) parieto-temporal (hippocampal sparing, 20.8%) and (iv) hippocampal-temporal (19.2%) atrophy patterns that were associated with phenotypes differing predominately in the presentation and progression of verbal memory and visuospatial impairments. These morphologically distinct subtypes are based on standardized brain regions, which are anatomically defined and freely accessible so as to validate its diagnostic accuracy and enhance the prediction of disease progression.
The Number or Type of Stimuli Used for Somatosensory Stimulation Affected the Modulation of Corticospinal Excitability
Authors: Kojima Sho , Miyaguchi Shota , Yokota Hirotake , Saito Kei , Inukai Yasuto , Otsuru Naofumi , Onishi Hideaki
Published: November 2021

Motor evoked potentials (MEPs) evoked by transcranial magnetic stimulation (TMS) a few milliseconds after this cortical activity following electrical stimulation (ES) result in an inhibition comparable to that by TMS alone; this is called short-latency afferent inhibition (SAI). Cortical activity is observed after mechanical tactile stimulation (MS) and is affected by the number of stimuli by ES. We determined the effects of somatosensory stimulus methods and multiple conditioning stimuli on SAI in 19 participants. In experiment 1, the interstimulus intervals between the conditioning stimulation and TMS were 25, 27 and 29 ms for ES and 28, 30 and 32 ms for MS. In experiment 2, we used 1, 2, 3 and 4 conditioning stimulations of ES and MS. The interstimulus interval between the ES or MS and TMS was 27 or 30 ms, respectively. In experiment 1, MEPs were significantly decreased in both the ES and MS conditions. In experiment 2, MEPs after ES were significantly decreased in all conditions. Conversely, MEPs after MS were significantly decreased after one stimulus and increased after four stimulations, indicating the SAI according to the number of stimuli. Therefore, the somatosensory stimulus methods and multiple conditioning stimuli affected the SAI.
The Efficacy of Interlimb-Coordinated Intervention on Gait and Motor Function Recovery in Patients with Acute Stroke: A Multi-Center Randomized Controlled Trial Study Protocol
Authors: Lo Wai-Leung Ambrose , Chen Dandan , Zhao Jiangli , Leng Yan , Bian Ruihao , Huang Wenzhu , Liang Yahui , Mao Yu-Rong , Huang Dong-Feng
Published: November 2021

Background: The efficacy of interlimb-coordinated training on gait and upper limb functional improvement remains unclear. The latest published randomized controlled trials have supported the potential benefits of interlimb-coordinated training to enhance gait function. Upper limb functional recovery may also benefit from interlimb-coordinated training since most everyday activities require the coordinated use of both hands to complete a task. This study investigates the efficacy of interlimb-coordinated training on gait and upper limb functional recovery over a short-medium term period. Methods: A total of 226 acute stroke patients will be recruited from four centres over four years. Patients will be randomly allocated to either conventional therapy or conventional therapy plus interlimb-coordinated training. Outcomes will be recorded at baseline, after 2 weeks of intervention, and at 3- and 6-months post-intervention. Gait speed is the primary outcome measure. Secondary outcome measures include Fugl–Meyer Assessment of Motor Recovery, Berg Balance Scale, Timed Up and Go test, Action Research Arm Test, electroencephalography, and magnetic resonance imaging. Conclusion: The results of this trial will provide an in-depth understanding of the efficacy of early interlimb-coordinated intervention on gait and upper functional rehabilitation and how it may relate to the neural plasticity process.
Effects of Music Intervention on Stress in Concussed and Non-Concussed Athletes
Authors: Léonard Camille , Desaulniers-Simon Jeanne Marie , Tat Diana , De Beaumont Louis , Gosselin Nathalie
Published: November 2021

Sport-related concussion is a serious public health issue affecting millions of individuals each year. Among the many negative side effects, emotional symptoms, such as stress, are some of the most common. Stress management is repeatedly cited by expert groups as an important intervention for this population. It was shown that music has relaxing effects, reducing stress through the activation of brain areas involved in emotions and pleasure. The objective of this study was to explore the effects of a music-listening intervention compared with silence on experimentally induced stress in concussed and non-concussed athletes. To this aim, four groups of athletes (non-concussed music, non-concussed silence, concussed music, and concussed silence) performed the Trier Social Stress Test, for which both physiological (skin conductance level) and self-reported stress measurements were taken. No significant difference was found in the pattern of stress recovery for self-reported measurements. However, the skin conductance results showed greater and faster post-stress recovery after listening to music compared with silence for concussed athletes only. Taken together, these results suggest that music could be an efficient stress management tool to implement in the everyday life of concussed athletes to help them prevent stress accumulation.
Cost Modeling from the Contractor Perspective: Application to Residential and Office Buildings
Authors: Monteiro Francisco Pereira , Sousa Vitor , Meireles Inês , Oliveira Cruz Carlos
Published: November 2021

For the majority of the contractual arrangements used in construction projects, the owner is not responsible for the cost deviations due to the variability of labor productivity or material price, amongst many other aspects. Consequently, the cost performance of a project may be entirely distinct for the owner and the contractor. Since the majority of quantitative research on cost estimation and deviation found in the literature adopts the owners’ perspective, this research provides a contribution towards modeling costs and cost deviation from a contractor’s perspective. From an initial sample of 13 residential buildings and 10 office building projects, it was possible to develop models for cost estimation at the early stage of development, including both endogenous and exogenous variables. Although the sample is relatively small, the authors were able to fully analyze all the cost data, using no secondary sources of data (which is very frequent in cost modeling studies). The statistically significant variables in the cost estimation models were the areas above and below ground and the years following the 2008 financial crisis, including the international bailout (2011–2014) period. For estimating the unit cost, a nonlinear model was obtained with the number of underground and total floor, the floor ratio, and the years following the 2008 financial crisis, including the international bailout (2011–2014) period as predictors. For the office buildings, a statistically significant correlation was also found between the cost deviation and number of underground floors.
New Dimension to Green Buildings: Turning Green into Occupant Well-Being
Authors: Fu Yang , Wang Hongdi , Sun Wen , Zhang Xiaoling
Published: November 2021

A series of international conferences and initiatives, such as the Habitat III New Urban Agenda and UN Sustainable Development Goals, have urged industry, scholars, and policymakers to create an inclusive and sustainable built environment for all in the coming era of cities. Green building schemes, which have been gaining momentum over recent decades, are one of the most influential measures that have been taken to promote urban sustainability. However, due to disciplinary characteristics, most current studies share a techno-engineering focus. Seldom do they answer the question: will green buildings make a difference to the occupants? This paper explains how, and to what extent, green features and design contribute to different dimensions of occupant well-being by conducting a systematic and comprehensive review of current journal articles and industrial reports. It provides an alternative, occupant-oriented perspective to the conventional discourse. A conceptual framework is developed, revealing that green building aspects are linked to six dimensions (three subjective and three objective) of occupant well-being. It further shows how different green features are linked with these dimensions through a detailed examination of the literature. Finally, suggestions are provided based on the research findings for the direction of future green building development and empirical research.
Health-Related Quality of Life following Cytoreductive Radical Prostatectomy in Patients with De-Novo Oligometastatic Prostate Cancer
Authors: Chaloupka Michael , Stoermer Lina , Apfelbeck Maria , Buchner Alexander , Wenter Vera , Stief Christian G. , Westhofen Thilo , Kretschmer Alexander
Published: November 2021

(1) Background: local treatment of the primary tumor has become a valid therapeutic option in de-novo oligo-metastatic prostate cancer (PC). However, evidence regarding radical prostatectomy (RP) in this setting is still subpar, and the effect of cytoreductive RP on postoperative health-related quality of life (HRQOL) is still unclear. (2) Methods: for the current study, patients with de-novo oligo-metastatic PC (cM1-oligo), defined as ≤5 bone lesions in the preoperative staging, were included, and matched cohorts using the variables age, body-mass index (BMI), and pT-stage were generated. Patient-reported outcome measures (PROMS) were assessed pre- and postoperatively using the validated EORTC-QLQ-C30, IIEF-5, and ICIQ-SF questionnaires. The primary endpoint for univariate and multivariable analysis was good general HRQOL defined by previously validated cut-off values. (3) Results: in total, 1268 patients (n = 84 (7%) cM1-oligo) underwent RP between 2012 and 2020 at one tertiary care center. A matched cohort of 411 patients (n = 79 with oligo-metastatic bone disease (cM1-oligo) and n = 332 patients without clinical indication of metastatic disease (cM0)) was created. The median follow-up was 25mo. There was no significant difference in good general HRQOL rates between cM1-oligo-patients and cM0-patients before RP (45.6% vs. 55.2%, p = 0.186), and at time of follow-up (44% vs. 56%, p = 0.811). Global health status (GHS) worsened significantly in cM0-patients compared to baseline (−5, p = 0.001), whereas GHS did not change significantly in cM1-oligo-patients (+3.2, p = 0.381). In multivariate analysis stratified for good erectile function (IIEF5 > 18; OR 5.722, 95% CI 1.89–17.36, p = 0.002) and continence recovery (OR 1.671, 95% CI 1.03–2.70, p = 0.036), cM1-oligo was not an independent predictive feature for general HRQOL (OR 0.821, 95% CI 0.44–1.53, p = 0.536). (4) Conclusions: in this large contemporary retrospective analysis, we observed no significant difference in HRQOL in patients with the oligometastatic bone disease after cytoreductive radical prostatectomy, when compared to patients with localized disease at time of surgery.
Microtubule Targeting Agents in Disease: Classic Drugs, Novel Roles
Authors: Wordeman Linda , Vicente Juan Jesus
Published: November 2021

Microtubule-targeting agents (MTAs) represent one of the most successful first-line therapies prescribed for cancer treatment. They interfere with microtubule (MT) dynamics by either stabilizing or destabilizing MTs, and in culture, they are believed to kill cells via apoptosis after eliciting mitotic arrest, among other mechanisms. This classical view of MTA therapies persisted for many years. However, the limited success of drugs specifically targeting mitotic proteins, and the slow growing rate of most human tumors forces a reevaluation of the mechanism of action of MTAs. Studies from the last decade suggest that the killing efficiency of MTAs arises from a combination of interphase and mitotic effects. Moreover, MTs have also been implicated in other therapeutically relevant activities, such as decreasing angiogenesis, blocking cell migration, reducing metastasis, and activating innate immunity to promote proinflammatory responses. Two key problems associated with MTA therapy are acquired drug resistance and systemic toxicity. Accordingly, novel and effective MTAs are being designed with an eye toward reducing toxicity without compromising efficacy or promoting resistance. Here, we will review the mechanism of action of MTAs, the signaling pathways they affect, their impact on cancer and other illnesses, and the promising new therapeutic applications of these classic drugs.
Regenerating Damaged Myocardium: A Review of Stem-Cell Therapies for Heart Failure
Authors: Fan Dihan , Wu Hanrong , Pan Kaichao , Peng Huashan , Wu Rongxue
Published: November 2021

Cardiovascular disease (CVD) is one of the contributing factors to more than one-third of human mortality and the leading cause of death worldwide. The death of cardiac myocyte is a fundamental pathological process in cardiac pathologies caused by various heart diseases, including myocardial infarction. Thus, strategies for replacing fibrotic tissue in the infarcted region with functional myocardium have long been a goal of cardiovascular research. This review begins by briefly discussing a variety of somatic stem- and progenitor-cell populations that were frequently studied in early investigations of regenerative myocardial therapy and then focuses primarily on pluripotent stem cells (PSCs), especially induced-pluripotent stem cells (iPSCs), which have emerged as perhaps the most promising source of cardiomyocytes for both therapeutic applications and drug testing. We also describe attempts to generate cardiomyocytes directly from cardiac fibroblasts (i.e., transdifferentiation), which, if successful, may enable the pool of endogenous cardiac fibroblasts to be used as an in-situ source of cardiomyocytes for myocardial repair.
Bitter Taste Receptors Expression in Human Granulosa and Cumulus Cells: New Perspectives in Female Fertility
Authors: Semplici Bianca , Luongo Francesca Paola , Passaponti Sofia , Landi Claudia , Governini Laura , Morgante Giuseppe , De Leo Vincenzo , Piomboni Paola , Luddi Alice
Published: November 2021

Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system, we investigated the expression and localization of bitter taste receptors and the components of signal transduction cascade involved in the pathway of taste receptors in somatic follicular cells obtained from women undergoing assisted reproductive techniques. We found that TAS2R genes are expressed in both cumulus (CCs) and granulosa (GCs) cells, with TAS2R14 being the most highly expressed bitter receptor subtype. Interestingly, a slight increase in the expression of TAS2R14 and TAS2R43 was shown in both GCs and CCs in young women (p < 0.05), while a negative correlation may be established between the number of oocytes collected at the pickup and the expression of TAS2R43. Regarding α-gustducin and α-transducin, two Gα subunits expressed in the taste buds on the tongue, we provide evidence for their expression in CCs and GCs, with α-gustducin showing two additional isoforms in GCs. Finally, we shed light on the possible downstream transduction pathway initiated by taste receptor activation in the female reproductive system. Our study, showing for the first time the expression of taste receptors in the somatic ovarian follicle cells, significantly extends the current knowledge of the biological role of TAS2Rs for human female fertility.
Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish
Authors: Raby Ludivine , Völkel Pamela , Hasanpour Shaghayegh , Cicero Julien , Toillon Robert-Alain , Adriaenssens Eric , Van Seuningen Isabelle , Le Bourhis Xuefen , Angrand Pierre-Olivier
Published: November 2021

Polycomb repressive complex 2 (PRC2) mediates histone H3K27me3 methylation and the stable transcriptional repression of a number of gene expression programs involved in the control of cellular identity during development and differentiation. Here, we report on the generation and on the characterization of a zebrafish line harboring a null allele of eed, a gene coding for an essential component of the PRC2. Homozygous eed-deficient mutants present a normal body plan development but display strong defects at the level of the digestive organs, such as reduced size of the pancreas, hepatic steatosis, and a loss of the intestinal structures, to die finally at around 10–12 days post fertilization. In addition, we found that PRC2 loss of function impairs neuronal differentiation in very specific and discrete areas of the brain and increases larval activity in locomotor assays. Our work highlights that zebrafish is a suited model to study human pathologies associated with PRC2 loss of function and H3K27me3 decrease.
Mechanosensing and the Hippo Pathway in Microglia: A Potential Link to Alzheimer’s Disease Pathogenesis?
Authors: Bruno Lucrezia , Karagil Simge , Mahmood Almas , Elbediwy Ahmed , Stolinski Michael , Mackenzie Francesca E.
Published: November 2021

The activation of microglia, the inflammatory cells of the central nervous system (CNS), has been linked to the pathogenesis of Alzheimer’s disease and other neurodegenerative diseases. How microglia sense the changing brain environment, in order to respond appropriately, is still being elucidated. Microglia are able to sense and respond to the mechanical properties of their microenvironment, and the physical and molecular pathways underlying this mechanosensing/mechanotransduction in microglia have recently been investigated. The Hippo pathway functions through mechanosensing and subsequent protein kinase cascades, and is critical for neuronal development and many other cellular processes. In this review, we examine evidence for the potential involvement of Hippo pathway components specifically in microglia in the pathogenesis of Alzheimer’s disease. We suggest that the Hippo pathway is worth investigating as a mechanosensing pathway in microglia, and could be one potential therapeutic target pathway for preventing microglial-induced neurodegeneration in AD.
Molecular Mechanisms of Hypertensive Nephropathy: Renoprotective Effect of Losartan through Hsp70
Authors: Costantino Valeria Victoria , Gil Lorenzo Andrea Fernanda , Bocanegra Victoria , Vallés Patricia G.
Published: November 2021

Hypertensive nephrosclerosis is the second most common cause of end-stage renal disease after diabetes. For years, hypertensive kidney disease has been focused on the afferent arterioles and glomeruli damage and the involvement of the renin angiotensin system (RAS). Nonetheless, in recent years, novel evidence has demonstrated that persistent high blood pressure injures tubular cells, leading to epithelial–mesenchymal transition (EMT) and tubulointerstitial fibrosis. Injury primarily determined at the glomerular level by hypertension causes changes in post-glomerular peritubular capillaries that in turn induce endothelial damage and hypoxia. Microvasculature dysfunction, by inducing hypoxic environment, triggers inflammation, EMT with epithelial cells dedifferentiation and fibrosis. Hypertensive kidney disease also includes podocyte effacement and loss, leading to disruption of the filtration barrier. This review highlights the molecular mechanisms and histologic aspects involved in the pathophysiology of hypertensive kidney disease incorporating knowledge about EMT and tubulointerstitial fibrosis. The role of the Hsp70 chaperone on the angiotensin II–induced EMT after angiotensin II type 1 receptor (AT1R) blockage, as a possible molecular target for therapeutic strategy against hypertensive renal damage is discussed.
Much Ado about Sleep: Current Concepts on Mechanisms and Predisposition to Pediatric Obstructive Sleep Apnea
Authors: Saint-Fleur Ashley L. , Christophides Alexa , Gummalla Prabhavathi , Kier Catherine
Published: November 2021

Obstructive Sleep Apnea (OSA) is a form of sleep-disordered breathing characterized by upper airway collapse during sleep resulting in recurring arousals and desaturations. However, many aspects of this syndrome in children remain unclear. Understanding underlying pathogenic mechanisms of OSA is critical for the development of therapeutic strategies. In this article, we review current concepts surrounding the mechanism, pathogenesis, and predisposing factors of pediatric OSA. Specifically, we discuss the biomechanical properties of the upper airway that contribute to its primary role in OSA pathogenesis and examine the anatomical and neuromuscular factors that predispose to upper airway narrowing and collapsibility.
High Mortality among Premature Neonates with Positive Blood Culture Neonatal Sepsis in a Tertiary Hospital, Tanzania: A Call for Action
Authors: Msanga Delfina R. , Parpia Fatema , Konje Eveline T. , Hokororo Adolfine , Mshana Stephen E.
Published: November 2021

Well-documented vital signs are key in the prediction of sepsis in low- and middle-income countries. We determined prevalence, associated factors, and outcomes of positive blood culture sepsis in premature neonates at Bugando Medical Centre Mwanza, Tanzania. Temperature, oxygen saturation, heart rate, respiratory rate, and random blood glucose were repeatedly recorded at admission, 8 h, and 24 h in all 250 neonates enrolled. Clinical and microbiological data were collected from patient records followed by descriptive data analysis. The mean age of the neonates was 3 ± 5.2 days, with the majority (90%) aged <10 days. The prevalence of positive blood culture sepsis was 21.2% (95% CI: 16.1–26.2). The fluctuation of the random blood glucose (RBG) (aOR = 1.34, 95% CI: (1.07–1.67), p = 0.010), low oxygen saturation (aOR = 0.94, 95% CI: (0.88–0.99), p = 0.031), premature rupture of membrane aOR = 4.28, 95% CI: (1.71–10.71), p = 0.002), gestational age < 34 weeks (aOR = 2.73, 95% CI: (1.20–6.24), p = 0.017), and home delivery (aOR = 3.90, 95% CI: (1.07–14.19), p = 0.039) independently predicted positive blood culture. Significantly more deaths were recorded in neonates with a positive blood culture than those with a negative blood culture (32.1% vs. 5.1%, p < 0.001). In limited-resource settings, clinicians should use the vital signs and clinical information to initiate timely sepsis treatment among preterm neonates to prevent deaths and other morbidities.
Predictive Value of Red Blood Cell Distribution Width, Mean Platelet Volume and Platelet Distribution Width in Children with Acute Appendicitis
Authors: Antić Jelena , Jokić Radoica , Bukarica Svetlana , Lukić Ivana , Dobrijević Dejan , Rakić Goran , Pajić Miloš , Trajković Veličko , Milenković Marina
Published: November 2021

Background and Objectives: Acute appendicitis in pediatric patients is one of the most common surgical emergencies, but the early diagnosis still remains challenging. The aim of this study was to determine the predictive value of Red blood cell distribution width (RDW), Mean platelet volume (MPV) and Platelet distribution width (PDW) in children with acute appendicitis. Materials and Methods: This study was a retrospective assessment of laboratory findings (RDW, MPV, PDW) of patients who underwent surgical treatment for acute appendicitis from January 2019 to December 2020. Result: During this period, 223 appendectomies were performed at our Institute. In 107 (43%) cases appendicitis was uncomplicated, while in 116 (46.6%) it was complicated. WBC and RDW/MPV ratio were significant parameters for the diagnosis of acute appendicitis with cut-off values of 12.86 (susceptibility: 66.3%; specificity: 73.2%) and 1.64 (susceptibility: 59.8%; specificity: 71.9%), respectively. WBC and RDW/RBC ratio were independent variables for the diagnosis of complicated appendicitis. The cut-off values were 15.05 for WBC (sensitivity: 60.5%; specificity: 70.7%) and 2.5 for RDW/RBC ratio (sensitivity: 72%; specificity: 52.8%). Conclusions: WBC is an important predictor of appendicitis and complicated appendicitis. RDW, MPV and PDW alone have no diagnostic value in pediatric acute appendicitis or predicting the degree of appendix inflammation. However, the RDW/MPV ratio can be an important predictor of appendix inflammation, with higher values in patients with more severe appendix inflammation. RDW/RBC ratio may be an important predictor of complicated appendicitis.
Crystal Structures, Thermal and Luminescent Properties of Gadolinium(III) Trans-1,4-cyclohexanedicarboxylate Metal-Organic Frameworks
Authors: Demakov Pavel A. , Vasileva Alena A. , Lazarenko Vladimir A. , Ryadun Alexey A. , Fedin Vladimir P.
Published: November 2021

Four new gadolinium(III) metal-organic frameworks containing 2,2′-bipyridyl (bpy) or 1,10-phenanthroline (phen) chelate ligands and trans-1,4-cyclohexanedicarboxylate (chdc2−) were synthesized. Their crystal structures were determined by single-crystal X-ray diffraction analysis. All four coordination frameworks are based on the binuclear carboxylate building units. In the compounds [Gd2(bpy)2(chdc)3]·H2O (1) and [Gd2(phen)2(chdc)3]·0.5DMF (2), the six-connected {Ln2(L)2(OOCR)6} blocks form a 3D network with the primitive cubic (pcu) topology. In the compounds [Gd2(NO3)2(phen)2(chdc)2]·2DMF (3) and [Gd2Cl2(phen)2(chdc)2]·0.3DMF·2.2dioxane (4), the four-connected {Ln2(L)2(X)2OOCR)4} units (where X = NO3− for 3 or Cl− for 4) form a 2D square-grid (sql) network. The solid-state luminescent properties were investigated for the synthesized frameworks. Bpy-containing compound 1 shows no luminescence, possibly due to the paramagnetic quenching by Gd3+ cation. In contrast, the phenathroline-containing MOFs 2–4 possess yellow emission under visible excitation (λex = 460 nm) with the tuning of the characteristic wavelength by the coordination environment of the metal center.