bluuebunny/crossref_metadata_2025 huggingface dataset:
Reinforcement of Environmental DNA Based Methods (Sensu Stricto) in Biodiversity Monitoring and Conservation: A Review
Authors: Banerjee Pritam , Dey Gobinda , Antognazza Caterina M. , Sharma Raju Kumar , Maity Jyoti Prakash , Chan Michael W. Y. , Huang Yi-Hsun , Lin Pin-Yun , Chao Hung-Chun , Lu Chung-Ming , Chen Chien-Yen
Published: November 2021

Recently developed non-invasive environmental DNA-based (eDNA) techniques have enlightened modern conservation biology, propelling the monitoring/management of natural populations to a more effective and efficient approach, compared to traditional surveys. However, due to rapid-expansion of eDNA, confusion in terminology and collection/analytical pipelines can potentially jeopardize research progression, methodological standardization, and practitioner adoption in several ways. Present investigation reflects the developmental progress of eDNA (sensu stricto) including highlighting the successful case studies in conservation management. The eDNA technique is successfully relevant in several areas of conservation research (invasive/conserve species detection) with a high accuracy and authentication, which gradually upgrading modern conservation approaches. The eDNA technique related bioinformatics (e.g., taxon-specific-primers MiFish, MiBird, etc.), sample-dependent methodology, and advancement of sequencing technology (e.g., oxford-nanopore-sequencing) are helping in research progress. The investigation shows that the eDNA technique is applicable largely in (i) early detection of invasive species, (ii) species detection for conservation, (iii) community level biodiversity monitoring, (iv) ecosystem health monitoring, (v) study on trophic interactions, etc. Thus, the eDNA technique with a high accuracy and authentication can be applicable alone or coupled with traditional surveys in conservation biology. However, a comprehensive eDNA-based monitoring program (ecosystem modeling and function) is essential on a global scale for future management decisions.
Vitis OneGenE: A Causality-Based Approach to Generate Gene Networks in Vitis vinifera Sheds Light on the Laccase and Dirigent Gene Families
Authors: Pilati Stefania , Malacarne Giulia , Navarro-Payá David , Tomè Gabriele , Riscica Laura , Cavecchia Valter , Matus José Tomás , Moser Claudio , Blanzieri Enrico
Published: November 2021

The abundance of transcriptomic data and the development of causal inference methods have paved the way for gene network analyses in grapevine. Vitis OneGenE is a transcriptomic data mining tool that finds direct correlations between genes, thus producing association networks. As a proof of concept, the stilbene synthase gene regulatory network obtained with OneGenE has been compared with published co-expression analysis and experimental data, including cistrome data for MYB stilbenoid regulators. As a case study, the two secondary metabolism pathways of stilbenoids and lignin synthesis were explored. Several isoforms of laccase, peroxidase, and dirigent protein genes, putatively involved in the final oxidative oligomerization steps, were identified as specifically belonging to either one of these pathways. Manual curation of the predicted sequences exploiting the last available genome assembly, and the integration of phylogenetic and OneGenE analyses, identified a group of laccases exclusively present in grapevine and related to stilbenoids. Here we show how network analysis by OneGenE can accelerate knowledge discovery by suggesting new candidates for functional characterization and application in breeding programs.
Rosinidin Attenuates Lipopolysaccharide-Induced Memory Impairment in Rats: Possible Mechanisms of Action Include Antioxidant and Anti-Inflammatory Effects
Authors: Alshehri Sultan , Imam Syed Sarim
Published: November 2021

The investigation aimed to evaluate the favourable effects of rosinidin in lipopolysaccharide (LPS)-induced learning and memory impairment in rats. Adult Wistar rats (150–200 g) were segregated equally into four different groups and treated as below: Group 1 (normal) and Group 2 (LPS control) were administered orally with 3 mL of 0.5% SCMC (vehicle); Group 3 and Group 4 were test groups and orally administered with rosinidin lower dose (10 mg/kg) and higher dose 20 mg/kg. Daily, 1 h post-offer mentioned treatments, Group 1 animals were injected with normal saline (i.p.) and groups 2–4 were treated with 1 mg/kg/day of LPS. This treatment schedule was followed daily for 7 days. During the treatment, schedule rats were evaluated for spontaneous locomotor activity, memory, and learning abilities. The biochemical assessment was carried out of acetylcholine esterase (AChE), endogenous antioxidants (GSH, SOD, GPx, and catalase), oxidative stress marker MDA, neuroinflammatory markers (IL-6, IL-1β, TNF-α, and NF-κB), and BDNF. LPS-induced reduced spontaneous locomotor activity and memory impairment in the animals. Moreover, LPS reduced GSH, SOD, GPx, and catalase levels; altered activities of AChE; elevated levels of MDA, IL-6, IL-1β, TNF-α, and NF-κB; and attenuated the levels of BDNF in brain tissue. Administration of rosinidin to LPS-treated animals significantly reduced LPS-induced neurobehavioral impairments, oxidative stress, neuroinflammatory markers, and reversed the Ach enzyme activities and BDNF levels towards normal. Results demonstrated that rosinidin attenuates the effects of LPS on learning memory in rats.
Biocompatible and Biodegradable Polymer Optical Fiber for Biomedical Application: A Review
Authors: Wang Yue , Huang Yu , Bai Hongyi , Wang Guoqing , Hu Xuehao , Kumar Santosh , Min Rui
Published: November 2021

This article discusses recent advances in biocompatible and biodegradable polymer optical fiber (POF) for medical applications. First, the POF material and its optical properties are summarized. Then, several common optical fiber fabrication methods are thoroughly discussed. Following that, clinical applications of biocompatible and biodegradable POFs are discussed, including optogenetics, biosensing, drug delivery, and neural recording. Following that, biomedical applications expanded the specific functionalization of the material or fiber design. Different research or clinical applications necessitate the use of different equipment to achieve the desired results. Finally, the difficulty of implanting flexible fiber varies with its flexibility. We present our article in a clear and logical manner that will be useful to researchers seeking a broad perspective on the proposed topic. Overall, the content provides a comprehensive overview of biocompatible and biodegradable POFs, including previous breakthroughs, as well as recent advancements. Biodegradable optical fibers have numerous applications, opening up new avenues in biomedicine.
The Allocation of Vertical Attention in Patients with End-Stage Renal Disease Receiving Dialysis
Authors: Mańkowska Aleksandra , Heilman Kenneth M. , Biedunkiewicz Bogdan , Dębska-Ślizień Alicja , Williamson John B. , Harciarek Michał
Published: November 2021

Objectives: Alterations of spatial attention can have adverse effects, such a greater probability of accidents. Patients with end-stage renal disease (ESRD) receiving dialysis have stronger left-sided spatial attentional bias, suggesting that this disorder or treatment alters the brain networks that mediate spatial attention. The hemispheric networks that mediate the allocation of horizontal attention may also influence the allocation of vertical attention. However, the allocation of vertical spatial attention has not been studied in ESRD patients. Methods: Twenty-three ESRD patients receiving dialysis and 23 healthy right-handed controls performed line bisections using 24 vertical lines (24 cm long and 2 mm thick) aligned with the intersection of their midsagittal and coronal planes. Results: Hemodialyzed ESRD patients had a significantly greater upward bias than healthy controls. The magnitude of this bias was correlated with the duration of the kidney disease. Conclusions: The reason why upward attentional bias is increased in hemodialyzed ESRD patients is not known. Further research is needed to better understand the brain mechanism that might account for this bias, as well as its treatment. However, hemodialyzed ESRD patients and their families-caregivers should be made aware of this disorder to avoid accidents such as tripping.
Beliefs and Experiences of Individuals Following a Zero-Carb Diet
Authors: Protogerou Cleo , Leroy Frédéric , Hagger Martin S.
Published: November 2021

The adoption of carbohydrate-restrictive diets to improve health is increasing in popularity, but there is a dearth of research on individuals who choose to severely restrict or entirely exclude carbohydrates. The present study investigated the beliefs and experiences of individuals following a diet that severely limits, or entirely excludes, dietary carbohydrates, colloquially known as a ‘zero-carb’ diet, for at least 6 months. Zero-carb dieters (n = 170) recruited via a social networking site completed an online qualitative survey prompting them to discuss their motives, rationale, and experiences of following a low-carb diet. Transcripts of participants’ responses were analyzed using inductive thematic analysis. Results revealed that participants’ decision to follow a zero-carb diet was driven by health concerns and benefits. Participants expressed a strong social identity and belongingness to online zero-carb communities. Participants reported strong intentions to follow the diet indefinitely. Shortcomings of the diet centered on experienced stigma; lack of support from healthcare providers and significant others; limited access to, and high cost of, foods; and limited scientific data on the diet. Further research into the benefits and shortcomings of zero-carb diets across settings and populations is warranted, and guidelines for healthcare professionals on how to support individuals following a zero-carb diet are needed.
The Role of Innate Immune Cells in Tumor Invasion and Metastasis
Authors: Huang Yu-Kuan , Busuttil Rita A. , Boussioutas Alex
Published: November 2021

Metastasis is considered one of the hallmarks of cancer and enhanced tumor invasion and metastasis is significantly associated with cancer mortality. Metastasis occurs via a series of integrated processes involving tumor cells and the tumor microenvironment. The innate immune components of the microenvironment have been shown to engage with tumor cells and not only regulate their proliferation and survival, but also modulate the surrounding environment to enable cancer progression. In the era of immune therapies, it is critical to understand how different innate immune cell populations are involved in this process. This review summarizes recent literature describing the roles of innate immune cells during the tumor metastatic cascade.
Boosting the H2 Production Efficiency via Photocatalytic Organic Reforming: The Role of Additional Hole Scavenging System
Authors: AlSalka Yamen , Al-Madanat Osama , Hakki Amer , Bahnemann Detlef W.
Published: November 2021

The simultaneous photocatalytic H2 evolution with environmental remediation over semiconducting metal oxides is a fascinating process for sustainable fuel production. However, most of the previously reported photocatalytic reforming showed nonstoichiometric amounts of the evolved H2 when organic substrates were used. To explain the reasons for this phenomenon, a careful analysis of the products and intermediates in gas and aqueous phases upon the photocatalytic hydrogen evolution from oxalic acid using Pt/TiO2 was performed. A quadrupole mass spectrometer (QMS) was used for the continuous flow monitoring of the evolved gases, while high performance ion chromatography (HPIC), isotopic labeling, and electron paramagnetic resonance (EPR) were employed to understand the reactions in the solution. The entire consumption of oxalic acid led to a ~30% lower H2 amount than theoretically expected. Due to the contribution of the photo-Kolbe reaction mechanism, a tiny amount of formic acid was produced then disappeared shortly after the complete consumption of oxalic acid. Nevertheless, a much lower concentration of formic acid was generated compared to the nonstoichiometric difference between the formed H2 and the consumed oxalic acid. Isotopic labeling measurements showed that the evolved H2, HD, and/or D2 matched those of the solvent; however, using D2O decreased the reaction rate. Interestingly, the presence of KI as an additional hole scavenger with oxalic acid had a considerable impact on the reaction mechanism, and thus the hydrogen yield, as indicated by the QMS and the EPR measurements. The added KI promoted H2 evolution to reach the theoretically predictable amount and inhibited the formation of intermediates without affecting the oxalic acid degradation rate. The proposed mechanism, by which KI boosts the photocatalytic performance, is of great importance in enhancing the overall energy efficiency for hydrogen production via photocatalytic organic reforming.
Efferocytosis during Skeletal Muscle Regeneration
Authors: Juban Gaëtan , Chazaud Bénédicte
Published: November 2021

Efferocytosis, i.e., engulfment of dead cells by macrophages, is a crucial step during tissue repair after an injury. Efferocytosis delineates the transition from the pro-inflammatory phase of the inflammatory response to the recovery phase that ensures tissue reconstruction. We present here the role of efferocytosis during skeletal muscle regeneration, which is a paradigm of sterile tissue injury followed by a complete regeneration. We present the molecular mechanisms that have been described to control this process, and particularly the metabolic control of efferocytosis during skeletal muscle regeneration.
The Retina: A Window into the Brain
Authors: Ptito Maurice , Bleau Maxime , Bouskila Joseph
Published: November 2021

In the course of evolution, animals have obtained the capacity to perceive and encode their environment via the development of sensory systems such as touch, olfaction, audition, and vision [...]
Mesenchymal Stromal Cells for the Treatment of Interstitial Lung Disease in Children: A Look from Pediatric and Pediatric Surgeon Viewpoints
Authors: Pelizzo Gloria , Silvestro Serena , Avanzini Maria Antonietta , Zuccotti Gianvincenzo , Mazzon Emanuela , Calcaterra Valeria
Published: November 2021

Mesenchymal stromal cells (MSCs) have been proposed as a potential therapy to treat congenital and acquired lung diseases. Due to their tissue-regenerative, anti-fibrotic, and immunomodulatory properties, MSCs combined with other therapy or alone could be considered as a new approach for repair and regeneration of the lung during disease progression and/or after post- surgical injury. Children interstitial lung disease (chILD) represent highly heterogeneous rare respiratory diseases, with a wild range of age of onset and disease expression. The chILD is characterized by inflammatory and fibrotic changes of the pulmonary parenchyma, leading to gas exchange impairment and chronic respiratory failure associated with high morbidity and mortality. The therapeutic strategy is mainly based on the use of corticosteroids, hydroxychloroquine, azithromycin, and supportive care; however, the efficacy is variable, and their long-term use is associated with severe toxicity. The role of MSCs as treatment has been proposed in clinical and pre-clinical studies. In this narrative review, we report on the currently available on MSCs treatment as therapeutical strategy in chILD. The progress into the therapy of respiratory disease in children is mandatory to ameliorate the prognosis and to prevent the progression in adult age. Cell therapy may be a future therapy from both a pediatric and pediatric surgeon’s point of view.
Hematopoietic Stem Cell Gene Therapy for Cystinosis: From Bench-to-Bedside
Authors: Cherqui Stephanie
Published: November 2021

Cystinosis is an autosomal recessive metabolic disease that belongs to the family of lysosomal storage disorders. The gene involved is the CTNS gene that encodes cystinosin, a seven-transmembrane domain lysosomal protein, which is a proton-driven cystine transporter. Cystinosis is characterized by the lysosomal accumulation of cystine, a dimer of cysteine, in all the cells of the body leading to multi-organ failure, including the failure of the kidney, eye, thyroid, muscle, and pancreas, and eventually causing premature death in early adulthood. The current treatment is the drug cysteamine, which is onerous and expensive, and only delays the progression of the disease. Employing the mouse model of cystinosis, using Ctns−/− mice, we first showed that the transplantation of syngeneic wild-type murine hematopoietic stem and progenitor cells (HSPCs) led to abundant tissue integration of bone marrow-derived cells, a significant decrease in tissue cystine accumulation, and long-term kidney, eye and thyroid preservation. To translate this result to a potential human therapeutic treatment, given the risks of mortality and morbidity associated with allogeneic HSPC transplantation, we developed an autologous transplantation approach of HSPCs modified ex vivo using a self-inactivated lentiviral vector to introduce a functional version of the CTNS cDNA, pCCL-CTNS, and showed its efficacy in Ctns−/− mice. Based on these promising results, we held a pre-IND meeting with the Food and Drug Administration (FDA) to carry out the FDA agreed-upon pharmacological and toxicological studies for our therapeutic candidate, manufacturing development, production of the GMP lentiviral vector, design Phase 1/2 of the clinical trial, and filing of an IND application. Our IND was cleared by the FDA on 19 December 2018, to proceed to the clinical trial using CD34+ HSPCs from the G-CSF/plerixafor-mobilized peripheral blood stem cells of patients with cystinosis, modified by ex vivo transduction using the pCCL-CTNS vector (investigational product name: CTNS-RD-04). The clinical trial evaluated the safety and efficacy of CTNS-RD-04 and takes place at the University of California, San Diego (UCSD) and will include up to six patients affected with cystinosis. Following leukapheresis and cell manufacturing, the subjects undergo myeloablation before HSPC infusion. Patients also undergo comprehensive assessments before and after treatment to evaluate the impact of CTNS-RD-04 on the clinical outcomes and cystine and cystine crystal levels in the blood and tissues for 2 years. If successful, this treatment could be a one-time therapy that may eliminate or reduce renal deterioration as well as the long-term complications associated with cystinosis. In this review, we will describe the long path from bench-to-bedside for autologous HSPC gene therapy used to treat cystinosis.
Cord Blood Platelet Gel as a Treatment of Occipital Pressure Injuries in Newborns: Report of Two Cases
Authors: Ferrario Silvia , Zorz Alessia , Sorrentino Gabriele , Villa Stefania , Cavalli Riccardo , Mosca Fabio , Plevani Laura , Ghirardello Stefano
Published: November 2021

Background: A Pressure Ulcer (PU) is a severe event and could create discomfort to newborns. In newborns, one of mostly stricken location by PU is occipital area. Recent studies have highlighted that Cord Blood Platelet Gel (CBPG) might be a better alternative compared to traditional treatment. We report two cases of occipital PU treated with CBPG. Case report: Two male infants showing occipital PU were treated with standard local treatment, but no improvement was observed. After parental informed consent was obtained, CBPG application on PU was performed every 48 h. In these two cases of PU, a fast improvement in healing was observed since the first application of CBPG. The PU healed resulted in a scar after 53 and 50 days (Case 1 and Case 2, respectively) from development. No complications or infections were reported. Conclusions: CBPG contains many angiogenetic and growth factors, these characteristics make it indicated in treating soft tissue injuries. It would seem to be safe and an effective treatment of neonatal PUs reducing the time of the healing and the hospitalization and the infectious risks. Further studies are needed to evaluate long term aesthetic and functional results of PU treated with CBPG.
Study on the Optimum Steel Slag Content of SMA-13 Asphalt Mixes Based on Road Performance
Authors: Chen Wei , Wei Jincheng , Xu Xizhong , Zhang Xiaomeng , Han Wenyang , Yan Xiangpeng , Hu Guiling , Lu Zizhao
Published: November 2021

To reduce the use of aggregates such as limestone and basalt, this paper used steel slag to replace some of the limestone aggregates in the production of SMA-13 asphalt mixes. The optimum content of steel slag in the SMA-13 asphalt mixes was investigated, and the performance of these mixes was evaluated. Five SMA-13 asphalt mixes with varying steel slag content (0%, 25%, 50%, 75%, and 100%) were designed and prepared experimentally. The high-temperature stability, low-temperature crack resistance, water stability, dynamic modulus, shear resistance, and volumetric stability of the mixes were investigated using the wheel tracking, Hamburg wheel tracking, three-point bending, freeze–thaw splitting, dynamic modulus, uniaxial penetration, and asphalt mix expansion tests. The results showed that compared to normal SMA-13 asphalt mixes, the high-temperature stability, water stability, and shear resistance of the SMA-13 asphalt mixes increased and then decreased as the steel slag content increased. All three performance indicators peaked at 75% steel slag content, and the dynamic stability, freeze–thaw splitting ratio, and uniaxial penetration strength increased by 90.48%, 7.39%, and 88.08%, respectively; however, the maximum bending tensile strain, which represents the low-temperature crack resistance of the asphalt mix, decreased by 5.98%. The dynamic modulus of the SMA-13 asphalt mixes increased with increasing steel slag content, but the volume expansion at a 75% steel slag content was 0.446% higher than at a 0% steel slag content. Based on the experimental results, the optimum content of steel slag for SMA-13 asphalt mixes was determined to be 75%.
Evaporation-Induced Crystal Nucleation and Morphology of Dried Poly(Vinylidene Fluoride) Droplets
Authors: Liang Yongri , Wang Susu
Published: November 2021

The evaporation of a polymer solution droplet is important in solution-based polymer film fabrications, such as inkjet printing, spray coatings, and droplet casting, etc. In this work, we investigated the effect of droplet size, solvent evaporation rate, and concentration on the “coffee-ring” effect, crystal nucleation, polymorphism, and morphology of dried poly(vinylidene fluoride) (PVDF) solution droplets with the atomic force microscopy (AFM) and two-dimensional grazing incidence wide angle X-ray scattering (2D GIWAXS) method. We found that the crystal structure, morphology and crystal distribution in the center and edge regions of dried PVDF droplets were different due to the “coffee-ring” effect. The “coffee-ring” effect of dried PVDF droplets was mainly composited of accumulated crystals at the edge region of a droplet, which was mainly made by the crystallization of migrated chains. The interplay between the migration of chains and the crystallization and solidification of PVDF droplets significantly influenced the formation of the “coffee-ring”. In addition, our results showed that the decrease in droplet size and the controlling solvent evaporation rate were effective ways to improve the electroactive crystalline phases (β and γ-phases) nucleation and decrease the crystal size.
Learning Scenario to Promote Comprehension of the Meaning of Subtraction
Authors: Silva Ricardo , Martins Fernando , Costa Cecília , Cravino José , Lopes Joaquim Bernardino
Published: November 2021

The integration of virtual manipulatives in classroom practices facilitates student learning processes. For this, the teacher must understand how to support students in establishing mathematical connections between the manipulation and their interpretations of the representations. We present a learning scenario that integrates the use of virtual manipulatives in mathematical modelling tasks. It was designed and implemented during initial teacher training, with the aim of helping a group of first-year elementary school students to overcome difficulties identified in their comprehension of the meaning of subtraction. The research, following the principles of design-based research, included three distinct moments: an individual written pre-test, an intervention phase with six micro-cycles, and an individual written post-test. The analysis of the collected data—audio, screen recordings, and written productions—allowed us to identify the most influential structural characteristics of the learning scenario: mathematical communication, collaborative learning, self-regulation, and co-regulation of learning. Our results suggest that differentiated instruction, minimal guidance, and scaffolding played an important role in the support provided by the pre-service teacher to students in the mathematical modelling activities that integrated virtual manipulatives.
Optimal Size of a Smart Ultra-Fast Charging Station
Authors: Leone Carola , Longo Michela , Fernández-Ramírez Luis M.
Published: November 2021

An ever-increasing penetration of electric vehicles (EVs) on the roads inevitably leads to an ever-stringent need for an adequate charging infrastructure. The emerging ultra-fast charging (UFC) technology has the potential to provide a refueling experience similar to that of gasoline vehicles; hence, it has a key role in enabling the adoption of EVs for medium-long distance travels. From the perspective of the UFC station, the differences existing in the EVs currently on the market make the sizing problem more challenging. A suitably conceived charging strategy can help to address these concerns. In this paper, we present a smart charging station concept that, through a modular DC/DC stage design, allows the split of the output power among the different charging ports. We model the issue of finding the optimal charging station as a single-objective optimization problem, where the goal is to find the number of modular shared DC/DC converters, and where the power rate of each module ensures the minimum charging time and charging cost. Simulation results show that the proposed solution could significantly reduce the required installed power. In particular, they prove that with an installed power of 800 kW it is possible to satisfy the needs of a UFC station composed of 10 charging spots.
Multi-Layer Hybrid Fuzzy Classification Based on SVM and Improved PSO for Speech Emotion Recognition
Authors: Huang Shihan , Dang Hua , Jiang Rongkun , Hao Yue , Xue Chengbo , Gu Wei
Published: November 2021

Speech Emotion Recognition (SER) plays a significant role in the field of Human–Computer Interaction (HCI) with a wide range of applications. However, there are still some issues in practical application. One of the issues is the difference between emotional expression amongst various individuals, and another is that some indistinguishable emotions may reduce the stability of the SER system. In this paper, we propose a multi-layer hybrid fuzzy support vector machine (MLHF-SVM) model, which includes three layers: feature extraction layer, pre-classification layer, and classification layer. The MLHF-SVM model solves the above-mentioned issues by fuzzy c-means (FCM) based on identification information of human and multi-layer SVM classifiers, respectively. In addition, to overcome the weakness that FCM tends to fall into local minima, an improved natural exponential inertia weight particle swarm optimization (IEPSO) algorithm is proposed and integrated with fuzzy c-means for optimization. Moreover, in the feature extraction layer, non-personalized features and personalized features are combined to improve accuracy. In order to verify the effectiveness of the proposed model, all emotions in three popular datasets are used for simulation. The results show that this model can effectively improve the success rate of classification and the maximum value of a single emotion recognition rate is 97.67% on the EmoDB dataset.
Pre-Processing Filter Reflecting Human Visual Perception to Improve Saliency Detection Performance
Authors: Lee Kyungjun , Wee Seungwoo , Jeong Jechang
Published: November 2021

Salient object detection is a method of finding an object within an image that a person determines to be important and is expected to focus on. Various features are used to compute the visual saliency, and in general, the color and luminance of the scene are widely used among the spatial features. However, humans perceive the same color and luminance differently depending on the influence of the surrounding environment. As the human visual system (HVS) operates through a very complex mechanism, both neurobiological and psychological aspects must be considered for the accurate detection of salient objects. To reflect this characteristic in the saliency detection process, we have proposed two pre-processing methods to apply to the input image. First, we applied a bilateral filter to improve the segmentation results by smoothing the image so that only the overall context of the image remains while preserving the important borders of the image. Second, although the amount of light is the same, it can be perceived with a difference in the brightness owing to the influence of the surrounding environment. Therefore, we applied oriented difference-of-Gaussians (ODOG) and locally normalized ODOG (LODOG) filters that adjust the input image by predicting the brightness as perceived by humans. Experiments on five public benchmark datasets for which ground truth exists show that our proposed method further improves the performance of previous state-of-the-art methods.
Effect of Diagenetic Evolution and Hydrocarbon Charging on the Reservoir-Forming Process of the Jurassic Tight Sandstone in the Southern Junggar Basin, NW China
Authors: Zhou Tianqi , Wu Chaodong , Guan Xutong , Wang Jialin , Zhu Wen , Yuan Bo
Published: November 2021

Deeply buried sandstones in the Jurassic, Toutunhe Formation, are a crucial exploration target in the Junggar Basin, NW China, whereas, reservoir-forming process of sandstones in the Toutunhe Formation remain unknown. Focused on the tight sandstone of the Toutunhe Formation, the impacts of diagenesis and hydrocarbon charging on sandstone reservoir-forming process were clarified based on the comprehensive analysis of sedimentary characteristics, petrography, petrophysical characteristics, and fluid inclusion analysis. Three diagenetic facies developed in the Toutunhe sandstone reservoirs, including carbonate cemented facies (CCF), matrix-caused tightly compacted facies (MTCF), and weakly diagenetic reformed facies (WDF). Except the WDF, the CCF and the MTCF entered the tight state in 18 Ma and 9 Ma, respectively. There was only one hydrocarbon emplacing event in sandstone reservoir of the Toutunhe Formation, charging in 13 Ma to 8 Ma. Meanwhile, the source rock started to expel hydrocarbons and buoyancy drove the hydrocarbon via the Aika fault belt to migrate into sandstone reservoirs in the Toutunhe Formation. During the end of the Neogene, the paleo-oil reservoir in the Toutunhe Formation was destructed and hydrocarbons migrated to the sandstone reservoirs in the Ziniquanzi Formation; some paleo-oil reservoirs survived in the WDF. The burial pattern and change of reservoir wettability were major controlling factors of the sandstone reservoir-forming process. The buried pattern of the Toutunhe Formation in the western section of the southern Junggar Basin was “slow and shallow burial at early stage and rapid and deep burial at late stage”. Hence, pore capillary pressure was extremely low due to limited diagenetic reformation (average pore capillary pressures were 0.26 MPa). At the same time, high content of chlorite coating increased the lipophilicity of reservoirs. Therefore, hydrocarbons preferably charged into the WDF with low matrix content (average 4.09%), high content of detrital quartz (average 28.75%), high content of chlorite films (average 2.2%), and lower pore capillary pressures (average 0.03 MPa). The above conditions were favorable for oil and gas enrichment.
Duty to Address Climate Change Litigation Risks for Australian Energy Companies—Policy and Governance Issues
Authors: Pearce Prafula
Published: November 2021

The transition from fossil fuels to renewable energy requires cooperation from all, including corporations, shareholders, and institutional investors. The purpose of this paper is to explore climate change litigation risks for Australian energy companies and investors from a policy and governance perspective. Companies are increasingly reporting their climate policies to satisfy their shareholders and investor demands. In addition, the government and judiciary are making laws and decisions to support the Paris Agreement. This paper explores whether company directors can and, in some cases, should be considering the impact of climate change litigation risks on their business, or else risk breaching their obligation to exercise care and diligence under the Corporation Act 2001 (Cth, Australia). The paper concludes that in addition to reducing climate change litigation risks, Australian energy companies and institutional investment bodies that invest in Australian energy companies can make informed climate risk decisions by aligning their investments with the goal of net-zero or reduced emissions.
Constrain on Oil Recovery Stage during Oil Shale Subcritical Water Extraction Process Based on Carbon Isotope Fractionation Character
Authors: Zhao Rongsheng , Ren Luquan , Deng Sunhua , Sun Youhong , Chang Zhiyong
Published: November 2021

In this work, Huadian oil shale was extracted by subcritical water at 365 °C with a time series (2–100 h) to better investigate the carbon isotope fractionation characteristics and how to use its fractionation characteristics to constrain the oil recovery stage during oil shale in situ exploitation. The results revealed that the maximum generation of oil is 70–100 h, and the secondary cracking is limited. The carbon isotopes of the hydrocarbon gases show a normal sequence, with no “rollover” and “reversals” phenomena, and the existence of alkene gases and the CH4-CO2-CO diagram implied that neither chemical nor carbon isotopes achieve equilibrium in the C-H-O system. The carbon isotope (C1–C3) fractionation before oil generation is mainly related to kinetics of organic matter decomposition, and the thermodynamic equilibrium process is limited; when entering the oil generation area, the effect of the carbon isotope thermodynamic equilibrium process (CH4 + 2H2O ⇄ CO2 + 4H2) becomes more important than kinetics, and when it exceeds the maximum oil generation stage, the carbon isotope kinetics process becomes more important again. The δ13CCO2−CH4 is the result of the competition between kinetics and thermodynamic fractionation during the oil shale pyrolysis process. After oil begins to generate, δ13CCO2−CH4 goes from increasing to decreasing (first “turning”); in contrast, when exceeding the maximum oil generation area, it goes from decreasing to increasing (second “turning”). Thus, the second “turning” point can be used to indicate the maximum oil generation area, and it also can be used to help determine when to stop the heating process during oil shale exploitation and lower the production costs.
Acoustic Vibration Approach for Detecting Faults in Hydroelectric Units: A Review
Authors: Dao Fang , Zeng Yun , Zou Yidong , Li Xiang , Qian Jing
Published: November 2021

The health of the hydroelectric generator determines the safe, stable, and reliable operation of the hydropower station. In order to keep the hydroelectric generator in a better state of health and avoid accidents, it is crucial to detect its faults. In recent years, fault detection methods based on sound and vibration signals have gradually become research hotspots due to their high sensitivity, achievable continuous dynamic monitoring, and easy adaptation to complex environments. Therefore, this paper is a supplement to the existing state monitoring and fault diagnosis system of the hydroelectric generator; it divides the hydroelectric generator into two significant parts: hydro-generator and hydro-turbine, and summarizes the research and application of fault detect technology based on sound signal vibration in hydroelectric generator and introduces some new technology developments in recent years, and puts forward the existing problems in the current research and future development directions, and it is expected to provides some reference for the research on fault diagnosis of the hydroelectric generator.
A Modified Multiple-Mixing-Cell Method with Sub-Cells for MMP Determinations
Authors: Xu Lingfei , Li Huazhou
Published: November 2021

Minimum miscible pressure (MMP) is an essential design parameter of gas flooding for enhanced oil recovery (EOR) applications. Researchers have developed a number of methods for MMP computations, including the analytical methods, the slim-tube simulation method, and the multiple-mixing-cell (MMC) method. Among these methods, the MMC method is widely accepted for its simplicity, robustness, and moderate computational cost An important version of the MMC method is the Jaubert et al. method which has a much lower computational cost than the slim-tube simulation method. However, the original Jaubert et al. method suffers several drawbacks. One notable drawback is that it cannot be applied to the scenario where the oil-gas MMP is lower than the saturation pressure of the crude oil. In this work, we present a modified MMC method that is more versatile and robust than the original version. Our method can handle the scenario where the oil-gas MMP is lower than the saturation pressure of the crude oil. Besides, we propose a modified MMC model that can reduce the computational cost of MMP estimations. This modified model, together with a newly proposed pressure search algorithm, increases the MMP estimation accuracy of the modified method. We demonstrate the good performance of the modified MMC method by testing it in multiple case studies. A good agreement is obtained between the MMPs calculated by the modified method and the tie-line-based ones from the literature.
Influence of Oxymethylene Ethers (OMEn) in Mixtures with a Diesel Surrogate
Authors: Richter Sandra , Kathrotia Trupti , Braun-Unkhoff Marina , Naumann Clemens , Köhler Markus
Published: November 2021

Within this work the effects of blending oxymethylene ethers (OMEn) to a diesel surrogate (50 mol% n-dodecane, 30 mol% farnesane, and 20 mol% 1-methylnaphthalene) were investigated by performing two different types of experiments: measurements of the sooting propensity and of the laminar burning velocity, each in laminar premixed flames. For the sooting propensity, OME3, OME4, and OME5 were considered as blending compounds—each in mass fractions of 10%, 20%, and 30%. The sooting propensity was found to depend strongly on the OMEn blending grade but not on its chain length. In addition, the effect on the laminar burning velocity was studied for OME4 and the admixture of 30% OME4 with diesel surrogate for the first time. This admixture was found to lead to increased burning velocities; however, much less than might be foreseen when considering the respective values of the neat fuels.
Buck-Boost Single-Stage Microinverter for Building Integrated Photovoltaic Systems
Authors: Mathew Derick , Farrag Mohamed Emad , Naidu Rani Chinnappa , Muthu Rajesh Kumar , Sivaprakasam A , Somasundaram P
Published: November 2021

Microinverters for Building Integrated Photovoltaic (BIPV) systems must have had a small number of components, be efficient, and be reliable. In this context, a single-phase Buck-Boost Single-stage Microinverter (BBSM) for grid-connected BIPV systems is presented. The concept of topology is extracted from the buck-boost converter. The leakage current in the system is kept under control. It uses an optimal number of active and passive components to function at a high-efficiency level. The suggested topology provides a high level of reliability due to the absence of shoot-through problems. To validate the findings, a simulation in combination with an experimental system for a 70 W system is developed with the design approach. The efficiency of the microinverter, total harmonic distortion of the grid current are measured as 96.4% and 4.09% respectively. Finally, a comparison study has indicated the advantages and disadvantages of the suggested inverter.
Predictions of Vortex Flow in a Diesel Multi-Hole Injector Using the RANS Modelling Approach
Authors: Kumar Aishvarya , Nouri Jamshid , Ghobadian Ali
Published: November 2021

The occurrence of vortices in the sac volume of automotive multi-hole fuel injectors plays an important role in the development of vortex cavitation, which directly influences the flow structure and emerging sprays that, in turn, influence the engine performance and emissions. In this study, the RANS-based turbulence modelling approach was used to predict the internal flow in a vertical axis-symmetrical multi-hole (6) diesel fuel injector under non-cavitating conditions. The project aimed to predict the aforementioned vortical structures accurately at two different needle lifts in order to form a correct opinion about their occurrence. The accuracy of the simulations was assessed by comparing the predicted mean axial velocity and RMS velocity of LDV measurements, which showed good agreement. The flow field analysis predicted a complex, 3D, vortical flow structure with the presence of different types of vortices in the sac volume and the nozzle hole. Two main types of vortex were detected: the “hole-to-hole” connecting vortex, and double “counter-rotating” vortices emerging from the needle wall and entering the injector hole facing it. Different flow patterns in the rotational direction of the “hole-to-hole” vortices have been observed at the low needle lift (anticlockwise) and full needle lift (clockwise), due to their different flow passages in the sac, causing a much higher momentum inflow at the lower lift with its much narrower flow passage.
A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection
Authors: Zhu Liangliang , Hao Hongshun , Ding Chao , Gan Hanwei , Jiang Shuting , Zhang Gongliang , Bi Jingran , Yan Shuang , Hou Hongman
Published: November 2021

To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 101–1.3 × 107 CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes.
Influence of Cabbage (Brassica oleracea) Accession and Growing Conditions on Myrosinase Activity, Glucosinolates and Their Hydrolysis Products
Authors: Oloyede Omobolanle O. , Wagstaff Carol , Methven Lisa
Published: November 2021

Glucosinolates are secondary plant metabolites present in Brassica vegetables. The endogenous enzyme myrosinase is responsible for the hydrolysis of glucosinolates, yielding a variety of compounds, including health-promoting isothiocyanates. The influence of cabbage accession and growing conditions on myrosinase activity, glucosinolates (GSL) and their hydrolysis products (GHPs) of 18 gene-bank cabbage accessions was studied. Growing conditions, cabbage morphotype and accession all significantly affected myrosinase activity and concentration of glucosinolates and their hydrolysis products. In general, cabbages grown in the field with lower growth temperatures had significantly higher myrosinase activity than glasshouse samples. Profile and concentration of glucosinolates and their hydrolysis products differed across the accessions studied. Aliphatic glucosinolates accounted for more than 60 % of total glucosinolates in most of the samples assessed. Nitriles and epithionitriles were the most abundant hydrolysis products formed. The results obtained showed that consumption of raw cabbages might reduce the amount of beneficial hydrolysis products available to the consumer, as more nitriles were produced from hydrolysis compared to beneficial isothiocyanates. However, red and white cabbages contained high concentrations of glucoraphanin and its isothiocyanate, sulforaphane. This implies that careful selection of accessions with ample concentrations of certain glucosinolates can improve the health benefits derived from raw cabbage consumption.
Individual Oligogenic Background in p.D91A-SOD1 Amyotrophic Lateral Sclerosis Patients
Authors: Gentile Giulia , Perrone Benedetta , Morello Giovanna , Simone Isabella Laura , Andò Sebastiano , Cavallaro Sebastiano , Conforti Francesca Luisa
Published: November 2021

The p.D91A is one of the most common ALS-causing SOD1 mutations and is known to be either recessive or dominant. The homozygous phenotype is characterized by prolonged survival and slow progression of disease, whereas the affected heterozygous phenotypes can vary. To date, no genetic protective factors located close to SOD1 have been associated with the mild progressive homozygous phenotype. Using Next Generation Sequencing (NGS), we characterized a small cohort of sporadic and familial p.D91A-SOD1 heterozygous (n = 2) or homozygous (n = 5) ALS patients, to reveal any additional contributing variant in 39 ALS-related genes. We detected unique sets of non-synonymous variants, four of which were of uncertain significance and several in untranslated regions of ALS-related genes. Our results supported an individual oligogenic background underlying both sporadic and familial p.D91A cases irrespective of their p.D91A mutant alleles. We suggest that a comprehensive genomic view of p.D91A-SOD1 ALS patients may be useful in identifying emerging variants and improving disease diagnosis as well as guiding precision medicine.
Species-Specific Duplication and Adaptive Evolution of a Candidate Sex Pheromone Receptor Gene in Weather Loach
Authors: Zhong Lei , Wang Weimin , Cao Xiaojuan
Published: November 2021

The release and sensation of sex pheromone play a role in the reproductive success of vertebrates including fish. Previous studies have shown that the weather loach Misgurnus anguillicaudatus perceives sex pheromones by olfaction to stimulate courtship behavior. It was speculated that weather loaches use smell to recognize intraspecific mates. However, the identification of loach pheromone receptor has not been reported. By comparative transcriptomic approach, we found that the olfactory receptor gene or114-1 was male-biasedly expressed in the olfactory epithelium of M. anguillicaudatus, M. bipartitus and the closely related species Paramisgurnus dabryanus. This sex-biased expression pattern implicated that or114-1 presumably encoded a sex pheromone receptor in loaches. M. bipartitus and P. dabryanus, like zebrafish, possess one or114-1 only. However, in M. anguillicaudatus, or114-1 has two members: Ma_or114-1a and Ma_or114-1b. Ma_or114-1a, not Ma_or114-1b, showed sex-differential expression in olfactory epithelium. Ma_or114-1b has base insertions that delayed the stop codon, causing the protein sequence length to be extended by 8 amino acids. Ma_or114-1a was subject to positive selection resulting in adaptive amino acid substitutions, which indicated that its ligand binding specificity has probably changed. This adaptive evolution might be driven by the combined effects of sexual selection and reinforcement of premating isolation between the sympatric loach species.