bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Training Parameter Concept and Sample Size in Possibilistic c-Means Classifier for Pigeon Pea Specific Crop Mapping
Authors: Sivaraj Priyadarsini , Kumar Anil , Koti Shiva Reddy , Naik Parth
Published: February 2022

This research work aims to study the effect of training parameter concept and sample size in the process of classification by using a fuzzy Possibilistic c-Means (PCM) approach for Pigeon Pea specific crop mapping. For specific class extraction, the “mean” of the training data is considered as a training parameter of the classification algorithm. In this study, we proposed an “Individual Sample as Mean” (ISM) approach where the individual training sample is accounted as a mean parameter for the fuzzy PCM classifier. In order to avoid the spectral overlap of target Pigeon pea crop with other crops in the study area, a temporal indices database was generated from Sentinel 2A/2B satellite images acquired during the 2019–2020 Pigeon Pea crop cycle. The spectral dimensionality of temporal data was reduced to extract the required bands to achieve maximum enhancement of the target crop class in the temporal data. Further, the training sample size was increased to study the heterogeneity within the class in the classified output. The proposed ISM approach delivered a higher mean membership difference (MMD) between the Pigeon Pea crop and the co-cultivated Cotton crop as compared to the conventional mean method. This indicated that a better separation was achieved between the target crop and the spectrally similar crop grown, that were cultivated in the same study area. When the sample size was gradually increased from 5 to 60, the MMD values within the Pigeon Pea test fields remained in the range 0.013–0.02, thereby implying that the proposed algorithm works better even with a small number of training samples. The heterogeneity was better handled using the proposed ISM approach since the variance obtained within Pigeon Pea field was only 0.008, as compared to that of 0.02 achieved using the conventional mean approach.
Treatment for Severe Malaria: Post-Artesunate Delayed Haemolysis and Neutropenia
Authors: Martino Mariangela , Liberati Cecilia , Bua Benedetta , Barbieri Elisa , Costenaro Paola , Di Chiara Costanza , Giaquinto Carlo , De Canale Ettore , Rampon Osvalda , Donà Daniele
Published: February 2022

Parenteral artesunate (AS) is the WHO first-line treatment recommended in adults and children for severe malaria. Post-artesunate delayed haemolysis (PADH) is an uncommon adverse reaction to AS with a mechanism that is not fully understood, occurring in adults and children. Neutropenia is another possible finding after AS treatment, albeit rare. We present the case of a child who experienced both effects after treatment with AS for imported severe Falciparum malaria with very high parasitaemia. In addition, thirty-five paediatric cases of PADH, five cases of delayed anaemia without known haemolysis, and fourteen cases of neutropenia after artesunate treatment were identified from the literature review. PADH seems to be a dose-independent reaction and is not strongly related to hyperparasitaemia, although it is more frequent in this case. To date, the benefits of AS outweigh its potential side effects. However, haematological follow-up is mandatory to avoid possible complications from anaemia and neutropenia, especially in children treated with other contemporary drugs.
A Standardized Dataset of a Spontaneous Adverse Event Reporting System
Authors: Khaleel Mohammad Ali , Khan Amer Hayat , Ghadzi Siti Maisharah Sheikh , Adnan Azreen Syazril , Abdallah Qasem M.
Published: February 2022

One of the largest spontaneous adverse events reporting databases in the world is the Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS). Unfortunately, researchers face many obstacles in analyzing data from the FAERS database. One of the major obstacles is the unstructured entry of drug names into the FAERS, as reporters might use generic names or trade names with different naming structures from all over the world and, in some cases, with typographical errors. Moreover, report duplication is a known problem in spontaneous adverse event-reporting systems, including the FAERS database. Hence, thorough text processing for database entries, especially drug name entries, coupled with a practical case-deduplication logic, is a prerequisite to analyze the database, which is a time- and resource-consuming procedure. In this study, we provide a clean, deduplicated, and ready-to-import dataset into any relational database management software of the FAERS database up to September 2021. Drug names are standardized to the RxNorm vocabulary and normalized to the single active ingredient level. Moreover, a pre-calculated disproportionate analysis is provided, which includes the reporting odds ratio (ROR), proportional reporting ratio (PRR), Chi-squared analysis with Yates correction (x2), and information component (IC) for each drug-adverse event pair in the database.
1st SOHO Clinical and Biological School of Acute Leukemia
Authors:   Hematology Reports Editorial Office
Published: February 2022

Genomic and functional alterations of enzymatic activity drive cancer metabolic reprogramming along with mutations of tumor suppressors and oncogenes [...]
Low R/FR Ratio Affects Pakchoi’s Growth and Nitrate Content under Excess Nitrate Stress
Authors: Chen Libang , Huang Jia , Liu Qinglin , Li Zelin , Chen Xu , Han Jiaxi , Gan Yirong , He Yuexuan , Jiang Chenxiang , Tang Yunxin , Zhou Xiaoting
Published: February 2022

Nitrate accumulation is one of the main factors of secondary soil salinization in protected horticulture in China. Previous studies have shown that a low red(R)/far-red (FR) ratio can improve the salt tolerance of plants under NaCl stress. However, the effect of a low R/FR ratio on plant growth under nitrate stress is not clear. In order to explore the effect of a low R/FR ratio on the adaptability of pakchoi under high nitrogen stress, the growth index, soluble protein content, soluble sugar content, nitrate content, nitrate reductase activity and Nia2 gene expression of pakchoi’s leaves were measured. The results showed that a high level of nitrogen (the addition of 80 mmol · L−1 NO3− (N80) and 160 mmol · L−1 NO3− (N160)) inhibited the growth of pakchoi and promoted the accumulation of osmoregulation substances and nitrate content, respectively. The reduction of the R/FR ratio under high nitrogen stress (L80) increased the fresh weight of the plants under it by 19.0%, reduced the nitrate content in the leaves by 22.7%, increased the NR activity by 29.9%, and made the Nia2 gene expression more significant, compared with N80. There was a similar mitigation effect of a low R/FR ratio under 160 mmol · L−1 excessive NO3− stress. Therefore, the reduction of the R/FR ratio can effectively control the nitrate content and improve the adaptability of pakchoi under high nitrogen stress. Thus, there is a practical application prospect for a low R/FR ratio for the production of pakchoi under a high level of nitrogen.
Alternative Control of Tomato Wilt Using the Aqueous Extract of Calotropis procera
Authors: Abo-Elyousr Kamal A. M. , Ali Esmat F. , Sallam Nashwa M. A.
Published: February 2022

Calotropis procera (Aiton) Dryand (commonly known as the apple of sodom, calotrope, and giant milkweed) is an evergreen, perennial shrub of the Apocynaceae family, and is mainly found in arid and semi-arid regions. Previous studies have established the toxic effects of Calotropis procera (C. procera) (Aiton). Its extract is used as one of the vital alternatives to pesticides due to its effective impacts on several pathogens and to combat insect pests that cause severe damage to several crops, but so far, its effects on combating Fusarium oxysporum f. sp. lycopersici (FOL) have not yet been evaluated. Therefore, the current study focuses on evaluating the effects of the aqueous extract of this plant on FOL by studying physicochemical indicators. In Saudi Arabia and many other countries, such disease is considered as one of the critical factors affecting tomato growth and production. For the control of Fusarium wilt disease (Fwd), many strategies have been developed, but are limited in their use. Ten isolates of the pathogens were obtained from diseased tomato plants. These isolates were able to exhibit symptoms of tomato wilt, and they varied in their virulence. Isolate No. 5 caused the highest severity of the disease (73%). According to these results, we used this isolate in the following experiment, which was identified by ITS sequences as F. oxysporum f. sp. lycopersici. Following intensive screening studies, we selected aqueous plant extract of C. procera to study its effectiveness against Fwd of tomato plants and their antifungal activity or induce systemic resistance in plants. All concentrations of C. procera extracts suppressed growth of the pathogen. The highest reduction of mycelia growth was obtained by aqueous extract at 15%, it reduced the pathogen growth to 70.2%, relative to the control. In greenhouse conditions, the aqueous C. procera extract at 5, 10, and 15% significantly reduced Fwd of the tomato compared to the infected control (52.01, 69.86, and 83.6%, respectively). The use of aqueous C. procera extract at 5, 10, and 15% significantly enhanced the fresh and dry weight of tomato plants (g plant−1) compared to inoculated plants (for fresh weight: 50, 66.6, and 86.6 and for dry weight: 60, 100, and 120%, respectively). Total phenols and flavonoids as well as antioxidant enzymes were increased in inoculated or non-inoculated tomato plants after being treated with aqueous extracts of C. procera.
Projecting Lifetime Health Outcomes and Costs Associated with the Ambient Fine Particulate Matter Exposure among Adult Women in Korea
Authors: Choi Gyeyoung , Kim Yujeong , Shin Gyeongseon , Bae SeungJin
Published: February 2022

We sought to estimate the lifetime healthcare costs and outcomes associated with the exposure to the escalated concentration of fine particulate matter (particle size < 2.5 μm, PM2.5) among adult Korean women. We adapted a previously developed Markov model, and a hypothetical cohort composed of Korean women was exposed to either a standard (15 μg/m3) or increased (25 μg/m3) concentration of PM2.5. The time horizon of the analysis was 60 years, and the cycle length was 1 year. The outcomes were presented as direct healthcare costs and quality-adjusted life years (QALYs), and costs were discounted annually at 5%. Deterministic and probabilistic sensitivity analyses were performed. The model estimated that when the exposure concentration was increased by 10 μg/m3, the lifetime healthcare cost increased by USD 9309, which is an 11.3% increase compared to the standard concentration group. Women exposed to a higher concentration of PM2.5 were predicted to live 30.64 QALYs, compared to 32.08 QALYs for women who were exposed to the standard concentration of PM2.5. The tendency of a higher cost and shorter QALYs at increased exposure was consistent across a broad range of sensitivity analyses. The negative impact of PM2.5 was higher on cost than on QALYs and accelerated as the exposure time increased, emphasizing the importance of early intervention.
Impact of Natural Disasters on Mental Health: A Cross-Sectional Study Based on the 2014 China Family Panel Survey
Authors: Zhang Rui , Zhang Yunzhi , Dai Zhe
Published: February 2022

Mental health problems are a leading cause of disability in both developed and developing countries, and the consequences of mental health disorders for individuals, families, and society as a whole could be severe and costly. To supplement relevant research and provide insightful policy suggestions to families, government and societies, this study investigates the nexus between natural disasters and mental health for middle-aged and older adults in rural China. Based on data of 8721 observations from the 2014 China Family Panel Studies, we estimate the effects of natural disasters on mental health using ordinary least squares and propensity score matching. Our findings suggest that natural disasters have a significant negative effect on middle-aged and older adults’ mental health in the case of rural China. This effect is heterogeneous depending on individuals’ education level and their agricultural production status. Finally, individuals’ happiness and life satisfaction are shown to be the potential mechanism through which the effect of natural disasters on mental health operates.
The Salvador Primary Care Longitudinal Study of Child Development (CohortDICa) Following the Zika Epidemic: Study Protocol
Authors: Santos Darci Neves , de Araújo Tânia Maria , dos Santos Leticia Marques , Kuper Hannah , Aquino Rosana , Da Silveira Ismael Henrique , Miranda Samilly Silva , Pereira Marcos , Werneck Guilherme Loureiro
Published: February 2022

This article describes the Salvador Primary Care Longitudinal Study of Child Development (CohortDICa). The exposed group was defined by confirmation of Congenital Zika Syndrome (CZS) diagnosed through computed tomography, magnetic resonance or transfontanellar ultrasound. A random selection of the 169 exposed children led to a subgroup of 120 children who were paired with children from the Live Birth Information System, according to birthdate, residence in the same street or neighborhood, and gestational age, resulting in 115 subjects in the non-exposed group. Following recruitment and before the participants completed 42 months, three measures were applied to assess cognitive, motor, and language performance, corresponding to three home visits. Social characteristics of the families and children, and the neurocognitive development of the children will be compared across the CZS exposed group (n = 147), the typical children with no exposure to CZS (n = 115) and the STORCH exposed group (Syphilis, Toxoplasma gondii, Rubella, Cytomegalovirus, and Herpes simplex) (n = 20). Primary Health Care (PHC) should include long-term care strategies for the care of children and family members, and might benefit from the research, teaching, and extension activities provided in this study. In the face of the consequences of the Zika virus epidemic, an opportunity arose to intervene in the integrated care of child development within PHC, including, on an equal basis, typical children and those with delays or disabilities in the first six years of life.
Importance and Reality of TDM for Antibiotics Not Covered by Insurance in Japan
Authors: Ebihara Fumiya , Hamada Yukihiro , Kato Hideo , Maruyama Takumi , Kimura Toshimi
Published: February 2022

Under the Japanese health insurance system, medicines undergoing therapeutic drug monitoring (TDM) can be billed for medical fees if they meet the specified requirements. In Japan, TDM of vancomycin, teicoplanin, aminoglycosides, and voriconazole, which are used for the treatment of infectious diseases, is common practice. This means the levels of antibiotics are measured in-house using chromatography or other methods. In some facilities, the blood and/or tissue concentrations of other non-TDM drugs are measured by HPLC and are applied to treatment, which is necessary for personalized medicine. This review describes personalized medicine based on the use of chromatography as a result of the current situation in Japan.
Exploring Unmet Information Needs of People with Parkinson’s Disease and Their Families: Focusing on Information Sharing in an Online Patient Community
Authors: Chu Hyeon Sik , Jang Hye Young
Published: February 2022

This study aimed to examine the unmet information needs of people with Parkinson’s disease and their family members by analyzing Parkinson’s disease-related posts in online communities. Data were collected from one of the largest online people with Parkinson’s disease communities used in South Korea. The word cloud, the main questions from the free-posting messages, as well as the frequently asked symptoms and side effects of the medication, were analyzed using content analysis. The commonly mentioned main questions from the free-posting messages have pertained to treatment-related information, such as effects and side effects of medication, deep brain stimulation, and complementary and alternative medicine. People with Parkinson’s disease and their families depend not only on health care providers but also on using online communities to find the information that they need. However, there is a need for treatment-specific information, such as anti-Parkinson drugs, deep brain stimulation, and complementary alternative therapies. As for the method of providing information for people with Parkinson’s disease and their families, it will be effective to provide tailored education services using online communities and social media by using their information needs and preferred resources.
A Tale of Four Danish Cities: Legionella pneumophila Diversity in Domestic Hot Water and Spatial Variations in Disease Incidence
Authors: Uldum Søren A. , Schjoldager Lars G. , Baig Sharmin , Cassell Kelsie
Published: February 2022

Denmark has one of the highest Legionnaires’ disease notification rates within Europe, averaging 4.7 cases per 100,000 population annually (2017 to 2020). The relatively high incidence of disease is not uniform across the country, and approximately 70% of all domestically acquired cases in Denmark are caused by Legionella pneumophila (LP) strains that are considered less virulent. The aim of this study was to investigate if colonization rates, levels of colonization, and/or types of LP present in hot water systems were associated with geographic differences in Legionnaires’ disease incidence. Domestic water systems from four cities in Denmark were analyzed via culture and qPCR. Serogrouping and sequence typing was performed on randomly selected isolates. Single nucleotide polymorphism was used to identify clonal relationship among isolates from the four cities. The results revealed a high LP colonization rate from 68% to 87.5% among systems, composed primarily of non-serogroup 1. LP serogroup 1 reacting with the monoclonal antibody (MAb) 3/1 was not identified in any of the systems tested, while MAb 3/1 negative serogroup 1 strains were isolated from 10 systems (9.6%). We hypothesize that a combination of factors influences the incidence rate of LD in each city, including sequence type and serogroup distribution, colonization rate, concentration of Legionella in Pre-flush and Flush samples, and potentially building characteristics such as water temperature measured at the point of use.
The Impact of the COVID-19 Pandemic on Dentistry and Dental Education: A Narrative Review
Authors: Goriuc Ancuta , Sandu Darius , Tatarciuc Monica , Luchian Ionut
Published: February 2022

Dentists and dental staff have an increased risk of airborne infection with pathogens such as SARS-CoV-2 since they are exposed to high levels of droplets and aerosols produced during specific dental procedures. Hence, new guidelines such as patient screening and temperature control, air purification, space, surface and hand sanitizing and the use of protective equipment and physical barriers have been successfully implemented. In addition, the use of teledentistry has expanded considerably in pediatric dentistry, orthodontics, oral medicine and periodontics in order to address oral and dental health issues during the COVID-19 pandemic while minimizing virus transmission. Thus, teleconsultation, telediagnosis, teletriage, teletreatment and telemonitoring have emerged as valuable tools not only in the delivery of care, but also in the academic and research training of dental health professionals. This narrative review summarizes the current literature on the impact of the pandemic on dental care, dental staff and dental education, with an emphasis on how newly emerging protocols and technologies can be successfully utilized as integral parts of various branches of the dental practice and their future implications without compromising patient care.
Displacement and Isolation: Insights from a Mental Stress Survey of Syrian Refugees in Houston, Texas, USA
Authors: Atrooz Fatin , Chen Tzuan A. , Biekman Brian , Alrousan Ghalya , Bick Johanna , Salim Samina
Published: February 2022

(1) Background: Syrians are the largest forcibly displaced population in the world. Approximately 20,000 Syrian refugees have resettled in the United States (US) since the civil war in Syria began in 2011, with an estimated 130 families resettling in Houston, Texas. We conducted a pilot study with the objective of examining the physical and mental well-being of the Houston Syrian refugee population. (2) Methods: Online surveys were conducted using psychometrically valid instruments including Afghan Symptom Checklist (ASC), Refugee Post-Migration Stress Scale (RPMSS), Perceived Stress Scale (PSS), and Self-Report Questionnaire (SRQ) (3) Results: According to independent t-tests, Syrian refugee females scored higher than males on ASC (37.78 vs. 31.64, p = 0.0446), particularly in the subscales of sadness with social withdrawal (28.89 vs. 24.31, p = 0.0495), and stress-induced reactivity (6.56 vs. 4.86, p = 0.0004). Similarly, females scored higher than males in RPMSS (60.54 vs. 45.15, p = 0.0022), including the social strain domain (8.08 vs. 5.18, p = 0.0204). In PSS and SRQ, Syrian refugee females reported comparable stress and distress scores as males. (4) Conclusions: Syrian refugee females reported higher stress and distress than males. Displacement from their home country and social strain were the major sources of stress in Syrian refugee females, as indicated in RPMSS.
Selecting External Controls for Internal Cases Using Stratification Score Matching Methods
Authors: Busgang Stefanie A. , Waller Lance A. , Colicino Elena , D’Agostino Ralph , Hertz-Picciotto Irva , Gennings Chris
Published: February 2022

Rare-disease registries can be useful for studying the associations between environmental exposures and disease severity, but often require the addition of a healthy comparison control group. Defining a surrogate control group, matched and balanced on potentially confounding variables, would allow for the comparison of exposure distributions with cases from a registry. In the present study, we assess whether controls selected externally, using stratification score (SS) matching, can serve as effective proxies for internal controls. In addition, we use methyl paraben (MEPB) to compare the estimated associations between an externally matched sample and case–control frequencies in a cohort with internally matched controls. We started with 225 eligible cases of autism spectrum disorder (ASD) from Childhood Autism Risks from Genetics and the Environment (CHARGE), 241 internal controls from CHARGE, and 265 external controls from the National Health and Nutrition Examination Survey (NHANES) cycles 2005–2016. We calculated the SSs using demographic covariates and matched 1:1 using a caliper method without a replacement. The distribution of the covariates and the mean squared error of the paired differences (MSEpaired) in the SSs between the internal and external group were similar (MSEpaired = 0.007 and 0.011, respectively). The association between MEPB and ASD compared to the controls was similar between the externally matched SS pairs and the original frequency matched cohort. Controls selected externally, via SS matching, can provide a comparable bias reduction to that provided by the internal controls, and therefore may be a useful strategy in situations when the internal controls are not available.
Associations between the Willingness to Donate Samples to Biobanks and Selected Psychological Variables
Authors: Pawlikowski Jakub , Wiechetek Michał , Majchrowska Anita
Published: February 2022

Over the past few decades, there has been a dynamic development of biobanks collecting human biological material and data. Advances in biomedical research based on biobanks, however, are highly dependent on the successful enrolment and participation of human subjects. Therefore, it is crucial to recognise those factors affecting the willingness of individuals to participate in biomedical research. There are very few studies pointing to the role of trust, preferred values and specific psychological factors. The aim of our study was the analysis of the most significant relationships between selected moral and psychological variables (i.e., preferred values, types of trust and personality) and willingness to donate biological material to biobanks. The research was carried out on a Polish representative national sample of 1100 people over 18 years of age. Statistical methods with regression models were used during the analyses. The willingness to donate samples to a biobank was associated with different types of trust and specific values. Based on regression analysis, the most important factors related to the willingness to donate material to biobanks seemed to be (1) trust towards scientists and doctors and (2) selected preferred values such as knowledge, self-development and tradition. Other values or personality traits did not seem to be as important in this context. The obtained results can be useful in building the social responsibility of biobankers and scientists, issuing more appropriate opinions by research ethics committees and planning better communication strategies between participants and biobanks.
Changes in Physical Activity and Sedentary Behavior before and during the COVID-19 Pandemic: A Swedish Population Study
Authors: Elvén Maria , Kerstis Birgitta , Stier Jonas , Hellström Charlotta , von Heideken Wågert Petra , Dahlen Micael , Lindberg Daniel
Published: February 2022

Governments have enforced measures to limit the spread of COVID-19 with varying degrees of success, which could affect people’s physical activity (PA) and sedentary behavior. This study aimed to examine changes in PA levels, types of PA, and sedentary behavior in the Swedish population before and during the COVID-19 pandemic. Associations between changed PA levels and demographical and behavioral determinants were also investigated. In December 2020, 1035 individuals (18–79 years old) completed a survey about their PA and sedentary behavior before and during the pandemic. Factors influencing their PA were also explored. Fifty-one percent of the sample reported reduced total PA, 18% had no change, and 31% increased their PA. Overall, organized PA decreased the most and sedentary behavior increased. The youngest and oldest age groups reported the greatest reduction in PA, while middle-aged groups reported the most increased PA. Men reported a larger increase in sedentary behavior than women. Mental and physical capability was associated with change in PA. In conclusion, this study indicates that, during the COVID-19 pandemic, the majority of the Swedish population have decreased PA levels with a concurrent increase in sedentary behavior, which may have negative health consequences. Interventions are recommended to address both PA and sedentary behavior, specifically to strengthen people’s ability to perform PA and focusing on the youngest and oldest age groups.
Adolescents’ Behaviors, Fitness, and Knowledge Related to Active Living before and during the COVID-19 Pandemic: A Repeated Cross-Sectional Analysis
Authors: Chen Senlin , Wang Baofu , Imagbe Stacy , Gu Xiangli , Androzzi Jared , Liu Yang , Yli-Piipari Sami R. , Hu Gang , Staiano Amanda E.
Published: February 2022

Background: Nearly all schools in the United States experienced shutdown followed by phased reopening during the COVID-19 pandemic, thereby limiting students’ opportunities for physical activity (PA). This study aimed to examine adolescents’ PA at school (PAS) and PA out-of-school (PAO), screen-based sedentary behaviors (SbSB), health-related fitness, and knowledge understanding about PA and fitness before and during the pandemic. Methods: Three rounds of data were collected: Time 1 pre-pandemic (January 2020; n = 405), Time 2 schools partially reopened (February 2021; n = 412), and Time 3 schools fully reopened (March 2021; n = 450). Adolescents completed the Youth Activity Profile, the 20 m Progressive Aerobic Cardiovascular Endurance Run (PACER) test and Plank test, and a written test, to measure the behaviors (T1, T2, T3), fitness (T2–T3), and knowledge (T1, T2, T3), respectively. Results: Inferential statistical analyses revealed a significant time effect for the behaviors and fitness. From T1 to T2 PAO decreased but PAS increased; whereas SbSB decreased at T3 compared to T1 and T2. Health-related fitness improved from T2 to T3. Further, the change patterns for SbSB varied by grade, and those for knowledge understanding varied by gender. Conclusion: The findings confirm the disruptive impact of the COVID-19 pandemic on adolescents’ active living but varied by school grade and gender. The favorable changes from T2 to T3 observed for fitness and other constructs may be partially attributable to an interrupted fitness education intervention. The findings may guide the design and evaluation of future interventions addressing the physical inactivity pandemic during public health crises (e.g., COVID-19).
Temporal Characteristics of Stress Signals Using GRU Algorithm for Heavy Metal Detection in Rice Based on Sentinel-2 Images
Authors: Zhang Yu , Liu Meiling , Kong Li , Peng Tao , Xie Dong , Zhang Li , Tian Lingwen , Zou Xinyu
Published: February 2022

Heavy metal stress, which is a serious environmental problem, affects both animal and human health through the food chain. However, such subtle stress information is difficult to detect in remote sensing images. Therefore, enhancing the stress signal is key to accurately identifying heavy metal contamination in crops. The aim of this study was to identify heavy metal stress in rice at a regional scale by mining the time-series characteristics of rice growth under heavy metal stress using the gated recurrent unit (GRU) algorithm. The experimental area was located in Zhuzhou City, Hunan Province, China. We collected situ-measured data and Sentinel-2A images corresponding to the 2019–2021 period. First, the spatial distribution of the rice in the study area was extracted using the random forest algorithm based on the Sentinel 2 images. Second, the time-series characteristics were analyzed, sensitive parameters were selected, and a GRU classification model was constructed. Third, the model was used to identify the heavy metals in rice and then assess the accuracy of the classification results using performance metrics such as the accuracy rate, precision, recall rate (recall), and F1-score (F1-score). The results showed that the GRU model based on the time series of the red-edge location feature index has a good classification performance with an overall accuracy of 93.5% and a Kappa coefficient of 85.6%. This study shows that regional heavy metal stress in crops can be accurately detected using the GRU algorithm. A combination of spectrum and temporal information appears to be a promising method for monitoring crops under various types of stress.
Development and Validation of the COVID-19 Knowledges and Behavior Questionnaire in a French Population (CoVQuest-CC)
Authors: Verot Elise , Bonjean Paul , Chaux Robin , Gagnaire Julie , Gagneux-Brunon Amandine , Pozzetto Bruno , Berthelot Philippe , Botelho-Nevers Elisabeth , Chauvin Franck
Published: February 2022

Background: The SARS-CoV-2 pandemic has led most countries to take restrictive measures affecting social activities and individual freedoms to limit viral transmission. It was shown that practical, motivational and social barriers impact on adherence to the isolation and social distancing measures advocated by the health authorities. The purpose of this study was to develop and validate a COVID-19 Knowledges and Behavior Questionnaire adapted to a teenager and adult French population. Methods: CoVQuest-CC was developed by a multidisciplinary team made of infectious diseases physicians, medical virologist, specialists of infectious control, experts of the questionnaires methodology, experts in public health and prevention, and statisticians. CoVQuest-CC was responded to by a big cohort from the general population during their participation in a massive SARS-CoV-2 screening campaign in 2021 in Saint-Etienne, France. Results: The confirmatory factorial analysis yielded good results (CFI = 0.94, TLI = 0.94, RMSEA = 0.04), and confirmed the five-dimensional structure of the questionnaire. Each dimension had a satisfying internal consistency, with Cronbach alphas of 0.83, 0.71, 0.65, 0.72 and 0.83 for transmission knowledge, barrier gesture respect, tests acceptability, home isolation possibility and test practicability, respectively. Conclusions: According to our knowledge, CoVQuest-CC is the first validated, consistent and reliable self-administrated French-specific questionnaire investigating the general population’s knowledge and attitudes towards COVID-19. It shows acceptable psychometric properties and can be use by Public Health teams or caregivers for public health and research purposes. Trial Registration: The study protocol was approved by the IRB ILE-DE-FRANCE 1 (No. IRB: I ORG0009918). All participants were given written and verbal information about the study and gave informed consent to participate. ClinicalTrials.gov identifier (NCT number): NCT04859023.
Association of High-Sensitivity C-Reactive Protein and Alcohol Consumption on Metabolic Syndrome in Korean Men
Authors: Lee Yong Woo , Kim Sung Soo , Suh Won Yoon , Seo Yu Ri , Lee Sami , Kim Hyun Gu , Kim Jong Sung , Yoon Seok Jun , Jung Jin Gyu
Published: February 2022

Background: This study aimed to examine the effect of both alcohol consumption and high-sensitivity C-reactive protein (hsCRP) on metabolic syndrome (MetS) in Korean men. Methods: A cohort of 364 men included in this study was divided into four groups according to the amount of alcohol they consumed: the nondrinkers (ND), low moderate drinkers (LM, ≤7 standard drinks per week), high moderate drinkers (HM, 7 to 14 drinks per week), and heavy drinkers (HD, >14 drinks per week). Logistic regression analyses were performed after adjusting for age, exercise, and smoking. Results: The risk of MetS in the LM group with a high hsCRP level (1.0 or more mg/dL) was not significant. However, the risks of MetS were significantly higher in the HM and HD groups with high hsCRP levels than that in the ND group. The odds ratios of MetS in the HM and HD groups with high hsCRP levels were 3.44 (95% confidence interval (CI), 1.25–9.52) and 3.14 (95% CI, 1.07–9.23), respectively. Conclusion: This study suggests that the risk of MetS is higher in men who consume more than seven drinks a week with high hsCRP levels than that in nondrinkers.
Perceptions of the COVID-19 Pandemic among Women with Infertility: Correlations with Dispositional Optimism
Authors: Dillard Amanda J. , Weber Ava E. , Chassee Amanda , Thakur Mili
Published: February 2022

People who are more optimistic may experience better psychological health during stressful times. The present study examined the perceptions and emotions surrounding the COVID-19 pandemic among American women who were experiencing fertility problems. We tested if dispositional optimism in these women was associated with less negative perceptions and emotions. We conducted a cross-sectional survey of patients from a single private infertility and reproductive clinic in an urban area in the Midwest, United States. Women, age 18 or older, primarily White and educated, who presented for an appointment to the clinic were invited to participate in an email-based survey. Respondents (N = 304) reported their perceived impact of the COVID-19 pandemic on fertility treatment, emotions associated with this impact, and perceived stress and depressive symptoms. They also completed measures of dispositional optimism and expectations for a future pregnancy. Findings indicated that women perceived an overall negative impact of the pandemic on their treatment plans, which was associated with more negative emotions, lower expectations of future pregnancy, and greater stress and depressive symptoms during the pandemic. However, further correlational analyses revealed that being higher in trait optimism was associated with perceiving a less negative impact of the pandemic, experiencing fewer negative emotions, and less overall stress and depressive symptoms. Although women with fertility problems have perceived the pandemic as negative and disruptive, those who are higher in optimism may be less affected.
Health Risk Assessment for the Residential Area Adjacent to a Former Chemical Plant
Authors: Wcisło Eleonora , Bronder Joachim
Published: February 2022

A health risk assessment was carried out for the residents of Łęgnowo-Wieś settlement adjacent to a former Zachem Chemical Plant, Bydgoszcz, Poland. Due to the unique Zachem site history and contamination profile, an innovative strategy for soil sampling and contaminant selection was applied. The novelty in the developed strategy consisted of selecting substances for the health risk assessment, taking into consideration the location and boundaries of the groundwater contamination plumes in relation to contamination sources. This allowed limiting the number of the analysed contaminants. The risk assessment focused on the surface soil of a residential area, which was divided into 20 sampling sectors and 6 backyards with wells from which water was used for watering edible plants. A total of 80 inorganic and organic substances were determined, including metals, phenol, aniline, BTEX, diphenyl sulphone, chloroaniline, epichlorohydrin, hydroxybiphenyl, nitrobenzene, octylphenols, toluenediamine, toluidine, 16 polycyclic aromatic hydrocarbons, tetrachloroethylene and trichloroethylene. For the health risk assessment, the United States Environmental Protection Agency’s deterministic method was applied. This applies conservative assumptions to obtain risk estimates protective for most of the potential receptors. Three exposure pathways were analysed: (1) incidental soil ingestion, (2) dermal contact with soil and (3) inhalation of fugitive soil particles and volatiles. In all sampling sectors and backyards, the total non-cancer risks (hazard index) were significantly lower than the acceptable level of 1. The acceptable cancer risk level for the single carcinogen of 1 × 10−5 was only insignificantly exceeded in the case of benzo(a)pyrene in three sectors and one backyard. The total cancer risks were lower than the acceptable level of 1 × 10−4 in all sampling sectors and all backyards. The findings show that the soil in the entire residential area is safe for the residents’ health and no remedial actions are required. However, since not all possible exposure pathways were analysed in this study, further research focused on assessing the health risk resulting from the consumption of locally grown food is strongly recommended.
Equity Carve-Outs, Dual Directors, and Internal Labor Markets
Authors: Fan Pengda
Published: February 2022

Given the prevalence of dual directors who serve simultaneously on the parent as well as the subsidiary board, it is important to examine their functions, a topic largely ignored in the existing literature. Exploring the functions of dual directors highlights equity carve-out objectives other than strategic refocusing. To examine our hypothesis, we first conducted an event study to examine stock market reaction to carve-out decisions. In addition, we compared subsidiaries’ performance after carve-outs between firms with dual directors and their matching firms based on the propensity score. We find evidence that the Japanese stock market reacts positively to the presence of dual directors who hold CEO positions in carve-out subsidiaries, especially when they are relatively young. Additionally, we find that carve-out subsidiaries led by young dual directors tend to outperform their matched counterparts in the long run. In contrast, when dual directors do not hold the CEO position, we find no evidence of the stock market reacting positively to them. The results of this study suggest that young CEOs appointed from the internal labor markets care more about long-term reputation, and can enhance shareholder wealth of both parent and subsidiary firms.
Nanoparticle Delivery Platforms for RNAi Therapeutics Targeting COVID-19 Disease in the Respiratory Tract
Authors: Zhang Yuan , Almazi Juhura G. , Ong Hui Xin , Johansen Matt D. , Ledger Scott , Traini Daniela , Hansbro Philip M. , Kelleher Anthony D. , Ahlenstiel Chantelle L.
Published: February 2022

Since December 2019, a pandemic of COVID-19 disease, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread across the globe. At present, the Food and Drug Administration (FDA) has issued emergency approval for the use of some antiviral drugs. However, these drugs still have limitations in the specific treatment of COVID-19, and as such, new treatment strategies urgently need to be developed. RNA-interference-based gene therapy provides a tractable target for antiviral treatment. Ensuring cell-specific targeted delivery is important to the success of gene therapy. The use of nanoparticles (NPs) as carriers for the delivery of small interfering RNA (siRNAs) to specific tissues or organs of the human body could play a crucial role in the specific therapy of severe respiratory infections, such as COVID-19. In this review, we describe a variety of novel nanocarriers, such as lipid NPs, star polymer NPs, and glycogen NPs, and summarize the pre-clinical/clinical progress of these nanoparticle platforms in siRNA delivery. We also discuss the application of various NP-capsulated siRNA as therapeutics for SARS-CoV-2 infection, the challenges with targeting these therapeutics to local delivery in the lung, and various inhalation devices used for therapeutic administration. We also discuss currently available animal models that are used for preclinical assessment of RNA-interference-based gene therapy. Advances in this field have the potential for antiviral treatments of COVID-19 disease and could be adapted to treat a range of respiratory diseases.
COVIDomics: The Proteomic and Metabolomic Signatures of COVID-19
Authors: Costanzo Michele , Caterino Marianna , Fedele Roberta , Cevenini Armando , Pontillo Mariarca , Barra Lucia , Ruoppolo Margherita
Published: February 2022

Omics-based technologies have been largely adopted during this unprecedented global COVID-19 pandemic, allowing the scientific community to perform research on a large scale to understand the pathobiology of the SARS-CoV-2 infection and its replication into human cells. The application of omics techniques has been addressed to every level of application, from the detection of mutations, methods of diagnosis or monitoring, drug target discovery, and vaccine generation, to the basic definition of the pathophysiological processes and the biochemical mechanisms behind the infection and spread of SARS-CoV-2. Thus, the term COVIDomics wants to include those efforts provided by omics-scale investigations with application to the current COVID-19 research. This review summarizes the diverse pieces of knowledge acquired with the application of COVIDomics techniques, with the main focus on proteomics and metabolomics studies, in order to capture a common signature in terms of proteins, metabolites, and pathways dysregulated in COVID-19 disease. Exploring the multiomics perspective and the concurrent data integration may provide new suitable therapeutic solutions to combat the COVID-19 pandemic.
Lycium barbarum Polysaccharides and Capsaicin Inhibit Oxidative Stress, Inflammatory Responses, and Pain Signaling in Rats with Dextran Sulfate Sodium-Induced Colitis
Authors: Chen Yu-Shan , Lian Yu Zhi , Chen Wen-Chao , Chang Chun-Chao , Tinkov Alexey A. , Skalny Anatoly V. , Chao Jane C.-J.
Published: February 2022

Ulcerative colitis (UC) is an inflammatory disease with chronic relapsing symptoms. This study investigated the effects of Lycium barbarum polysaccharides (LBP) and capsaicin (CAP) in dextran sulfate sodium (DSS)-induced UC rats. Rats were divided into normal, DSS-induced UC, and UC treated with 100 mg LBP/kg bw, 12 mg CAP/kg bw, or 50 mg LBP/kg bw and 6 mg CAP/kg bw. Rats were fed LBP or CAP orally by gavage for 4 weeks, and UC model was established by feeding 5% DSS in drinking water for 6 days during week 3. Oral CAP and mixture significantly reduced disease activity index. Oral LBP significantly decreased serum malondialdehyde, interleukin (IL)-6, colonic tumor necrosis factor (TNF)-α levels, and protein expression of transient receptor potential cation channel V1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1), but increased serum catalase activity. Oral CAP significantly suppressed serum IL-6, colonic TRPV1 and TRPA1 protein expression, but elevated IL-10 levels, serum superoxide dismutase and catalase activities. The mixture of LBP and CAP significantly reduced serum IL-6, colonic TNF-α and TRPA1 protein. In conclusion, administration of LBP and/or CAP attenuate DSS-induced UC symptoms through inhibiting oxidative stress, proinflammatory cytokines, and protein expression of TRPV1 and TRPA1.
Artificial Tears: Biological Role of Their Ingredients in the Management of Dry Eye Disease
Authors: Labetoulle Marc , Benitez-del-Castillo Jose Manuel , Barabino Stefano , Herrero Vanrell Rocio , Daull Philippe , Garrigue Jean-Sebastien , Rolando Maurizio
Published: February 2022

Dry eye disease (DED) is the most common ocular surface disease, characterized by insufficient production and/or instability of the tear film. Tear substitutes are usually the first line of treatment for patients with DED. Despite the large variety of tear substitutes available on the market, few studies have been performed to compare their performance. There is a need to better understand the specific mechanical and pharmacological roles of each ingredient composing the different formulations. In this review, we describe the main categories of ingredients composing tear substitutes (e.g., viscosity-enhancing agents, electrolytes, osmo-protectants, antioxidants, lipids, surfactants and preservatives) as well as their effects on the ocular surface, and we provide insight into how certain components of tear substitutes may promote corneal wound healing, and/or counteract inflammation. Based on these considerations, we propose an approach to select the most appropriate tear substitute formulations according to the predominant etiological causes of DED.
A Review of Alpha-1 Antitrypsin Binding Partners for Immune Regulation and Potential Therapeutic Application
Authors: O’Brien Michael E. , Murray Grace , Gogoi Debananda , Yusuf Azeez , McCarthy Cormac , Wormald Mark R. , Casey Michelle , Gabillard-Lefort Claudie , McElvaney Noel G. , Reeves Emer P.
Published: February 2022

Alpha-1 antitrypsin (AAT) is the canonical serine protease inhibitor of neutrophil-derived proteases and can modulate innate immune mechanisms through its anti-inflammatory activities mediated by a broad spectrum of protein, cytokine, and cell surface interactions. AAT contains a reactive methionine residue that is critical for its protease-specific binding capacity, whereby AAT entraps the protease on cleavage of its reactive centre loop, neutralises its activity by key changes in its tertiary structure, and permits removal of the AAT-protease complex from the circulation. Recently, however, the immunomodulatory role of AAT has come increasingly to the fore with several prominent studies focused on lipid or protein-protein interactions that are predominantly mediated through electrostatic, glycan, or hydrophobic potential binding sites. The aim of this review was to investigate the spectrum of AAT molecular interactions, with newer studies supporting a potential therapeutic paradigm for AAT augmentation therapy in disorders in which a chronic immune response is strongly linked.