bluuebunny/crossref_metadata_2025 huggingface dataset:
Glycemic Abnormalities in Pancreatic Cystic Lesions—A Single-Center Retrospective Analysis
Authors: Balaban Daniel Vasile , Coman Laura , Balaban Marina , Zoican Andreea , Pușcașu Danusia Adriana , Ayatollahi Simin , Mihălțeanu Emanuela , Costache Raluca Simona , Ioniță-Radu Florentina , Jinga Mariana
Published: May 2023

Background and Objectives: Glucose metabolism alterations are very common in solid pancreatic lesions, particularly in pancreatic cancer. Similarly, diabetes and especially new-onset diabetes (NOD) have been associated with the malignant transformation of pancreatic cysts. We aimed to assess the prevalence and relevant associations of glycemic abnormalities in pancreatic cystic lesions (PCLs) in a retrospective analysis. Materials and Methods: We retrospectively recruited all patients who underwent endoscopic ultrasound for a PCL over a period of 36 months (January 2018 to December 2021). Final diagnosis was set by means of tissue acquisition, surgery, follow-up, or board decision. Demographic and clinical data, laboratory workup, and imaging features were extracted from the patients’ charts according to a predefined protocol. We considered fasting blood glucose (FBG) and HbA1c values and stratified the patients as nondiabetic (FBG ≤ 99 mg/dL, HbA1c ≤ 5.6%, no history of glycemic abnormalities), prediabetic (FBG 100–125 mg/dL, HbA1c 5.7–6.4%), or diabetic (long-lasting diabetes or NOD). Results: Altogether, 81 patients were included, with a median age of 66 years, and 54.3% of them were male. The overall prevalence of fasting hyperglycemia was 54.3%, comprising 34.6% prediabetes and 22.2% diabetes, of which 16.7% had NOD. The mean FBG and HbA1c levels were higher in malignant and premalignant PCLs (intraductal papillary mucinous neoplasm (IPMN), mucinous cystic neoplasm (MCN), cystadenocarcinoma, and cystic neuroendocrine tumor) compared to the benign lesions (pseudocysts, walled-off necrosis, and serous cystadenoma): 117.0 mg/dL vs. 108.3 mg/dL and 6.1% vs. 5.5%, respectively. Conclusions: Hyperglycemia and diabetes are common in PCLs, with a high prevalence in premalignant and malignant cysts. Screening and follow-up for glycemic abnormalities should be routinely conducted for PCLs, as they can contribute to a tailored risk assessment of cysts.
Rapid Preparation of Superabsorbent Self-Healing Hydrogels by Frontal Polymerization
Authors: Qin Ying , Li Hao , Shen Hai-Xia , Wang Cai-Feng , Chen Su
Published: May 2023

Hydrogels have received increasing interest owing to their excellent physicochemical properties and wide applications. In this paper, we report the rapid fabrication of new hydrogels possessing a super water swelling capacity and self-healing ability using a fast, energy-efficient, and convenient method of frontal polymerization (FP). Self-sustained copolymerization of acrylamide (AM), 3-[Dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azaniumyl]propane-1-sulfonate (SBMA), and acrylic acid (AA) within 10 min via FP yielded highly transparent and stretchable poly(AM-co-SBMA-co-AA) hydrogels. Thermogravimetric analysis and Fourier transform infrared spectroscopy confirmed the successful fabrication of poly(AM-co-SBMA-co-AA) hydrogels with a single copolymer composition without branched polymers. The effect of monomer ratio on FP features as well as porous morphology, swelling behavior, and self-healing performance of the hydrogels were systematically investigated, showing that the properties of the hydrogels could be tuned by adjusting the chemical composition. The resulting hydrogels were superabsorbent and sensitive to pH, exhibiting a high swelling ratio of up to 11,802% in water and 13,588% in an alkaline environment. The rheological data revealed a stable gel network. These hydrogels also had a favorable self-healing ability with a healing efficiency of up to 95%. This work contributes a simple and efficient method for the rapid preparation of superabsorbent and self-healing hydrogels.
Metabolite Profiling and Transcriptome Analysis Explain the Difference in Accumulation of Bioactive Constituents in Taxilli Herba from Two Hosts
Authors: Yuan Jiahuan , Wu Nan , Cai Zhichen , Chen Cuihua , Zhou Yongyi , Chen Haijie , Xue Jia , Liu Xunhong , Wang Wenxin , Cheng Jianming , Li Li
Published: May 2023

Taxilli Herba (TH) is a semi-parasitic herb and the host is a key factor affecting its quality. Flavonoids are the main bioactive constituents in TH. However, studies on the difference in accumulation of flavonoids in TH from different hosts are vacant. In this study, integrated transcriptomic and metabolomic analyses were performed on TH from Morus alba L. (SS) and Liquidambar formosana Hance (FXS) to investigate the relationship between the regulation of gene expression and the accumulation of bioactive constituents. The results showed that a total of 3319 differentially expressed genes (DEGs) were screened in transcriptomic analysis, including 1726 up-regulated genes and 1547 down-regulated genes. In addition, 81 compounds were identified using ultra-fast performance liquid chromatography coupled with triple quadrupole-time of flight ion trap tandem mass spectrometry (UFLC-Triple TOF-MS/MS) analysis, and the relative contents of flavonol aglycones and glycosides were higher in TH from SS group than those from the FXS group. A putative biosynthesis network of flavonoids was created, combined with structural genes, and the expression patterns of genes were mostly consistent with the variation of bioactive constituents. It was noteworthy that the UDP-glycosyltransferase genes might participate in downstream flavonoid glycosides synthesis. The findings of this work will provide a new way to understand the quality formation of TH from the aspects of metabolite changes and molecular mechanism.
Genome-Wide Identification and Functional Analysis of NAP1 in Triticum aestivum
Authors: Feng Huimin , Wu Mila , Wang Ziqiong , Wang Xia , Chen Jianping , Yang Jian , Liu Peng
Published: May 2023

As a main molecular chaperone of histone H2A-H2B, nucleosome assembly protein 1 (NAP1) has been widely researched in many species. However, there is little research investigating the function of NAP1 in Triticum aestivum. To understand the capabilities of the family of NAP1 genes in wheat and the relationship between TaNAP1 genes and plant viruses, we performed comprehensive genome-wide analysis and quantitative real-time polymerase chain reaction (qRT-PCR) for testing expression profiling under hormonal and viral stresses. Our results showed that TaNAP1 was expressed at different levels in different tissues, with higher expression in tissues with high meristematic capacity, such as roots. Furthermore, the TaNAP1 family may participate in plant defense mechanisms. This study provides a systematic analysis of the NAP1 gene family in wheat and lays the foundation for further studies on the function of TaNAP1 in the response of wheat plants to viral infection.
Understanding the Spatial Variability of the Relationship between InSAR-Derived Deformation and Groundwater Level Using Machine Learning
Authors: Fu Guobin , Schmid Wolfgang , Castellazzi Pascal
Published: May 2023

The interferometric synthetic aperture radar (InSAR) technique was used in this study to derive the temporal and spatial information of ground deformation and explore its temporal correlation with groundwater dynamics. The random forest (RF) machine learning method was used to model the spatial variability of the temporal correlation and understand its influential contributors. The results showed that groundwater dynamics appeared to be an important factor in InSAR deformation at some bores where strong and positive correlations were observed. The RF model could explain up to 72% of spatial variances between InSAR deformation and groundwater dynamics. The spatial and temporal InSAR coherence (a proxy for the noise in InSAR results that is strongly related to vegetation) and soil moisture (difference, trend, and amplitude) were the most important factors explaining the spatial pattern of the temporal correlation between InSAR displacements and groundwater levels. This result confirms that noise sources (including deformation model fitting errors and radar signal decorrelation) and perturbation of the InSAR signal related to vegetation and surficial soils (clay content, moisture changes) should be accounted for when interpreting InSAR to support groundwater-related risk assessments and in groundwater resource management activities.
The Effects of Fertilizer Sources and Site Location on Greenhouse Gas Emissions from Creeping Bentgrass Putting Greens and Kentucky Bluegrass Roughs
Authors: Chapman Katy E. , Walker Kristina S.
Published: May 2023

Understanding greenhouse gas (GHG) emissions from turfgrass allows managers to make cultural management decisions to reduce GHG emissions. The objective of this study was to evaluate fertilizer source [urea (URE), polymer-encapsulated urea (POL), and milorganite (MIL)] and site location (green, wet rough, and dry rough) on GHG [carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O)] emissions. Greenhouse gas data, soil temperature, soil moisture, canopy greenness, and turfgrass quality were collected. High soil temperature and moisture were correlated with soil CO2 and N2O flux. The wet rough fluxed more soil CH4 across the 2-year study. The POL fluxed the highest amount of soil CO2, while POL and MIL fluxed the largest amount of soil N2O on the wet rough. Milorganite and POL increased canopy greenness in both roughs during the spring. On the green, URE produced greater canopy greenness in the spring and fall. Our results indicate that when soil moisture and temperature are high, turfgrass managers should employ methods of reducing soil temperatures that do not increase soil moisture to reduce GHG emissions. Under warm and wet conditions, gaseous losses of GHGs are accelerated with slow-release fertilizers.
Longitudinal Changes in the Parenting Stress of Mothers of Children with Cerebral Palsy and Their Relationship with Children’s Gross Motor Function System Levels
Authors: Park Eun-Young
Published: May 2023

Stress caused by children with disabilities harms the growth and development of children and their families. The present study aimed to investigate whether parenting stress of mothers of children with cerebral palsy changes and the relationship between children’s gross motor function level and changes in parenting stress. Data were collected from 162 children with cerebral palsy and their mothers over a 2-year period at three time points. Gross motor function and parenting stress were assessed using the Gross Motor Function Classification System and the Korean version of the Parenting Stress Index—Short Form. Linear latent growth curve models of Wave 2 and Wave 3 variation in parenting stress were constructed. The relationship between Gross Motor Function Classification System level and parenting stress was evaluated via latent growth modeling. The results showed that the linear variation models reflected the time evolution of parenting stress. There were individual differences in parenting stress at the initial level and no individual differences in changes in parenting stress. The relationship between the initial level and the change rate of parenting stress was not significant. Higher initial values of the Gross Motor Function Classification System level were associated with higher initial values of parenting stress, but not related to a change in parenting stress. The results showed that parenting stress of mothers with children with cerebral palsy decreased over time and that Gross Motor Function Classification System level was related to parenting stress level. Based on these findings, directions for further research are put forth.
The Influence of Gender Equity in Nursing Education Programs on Nurse Job Satisfaction
Authors: Shim Joohee , Park Da-In
Published: May 2023

(1) Background: One of the strategies to overcome the shortage of nurses is to minimize gender inequity in nursing culture, starting from the undergraduate education program. Although the number of men entering the nursing profession has increased over the years, the portion of male nurses remains low, particularly in Asian countries. Only a few studies have been conducted to identify gender inequity in nursing, and most of these studies used qualitative study design. Therefore, it is necessary to quantitatively identify gender equity in nursing education and the influence it has on nurse job satisfaction. (2) Methods: A total of 165 male nurses participated in this study. Validated questionnaires were used to assess gender equity in nursing education programs, nurse job satisfaction, nurse job esteem, and nursing professional pride. (3) Results: The mean score of gender equity in nursing programs was 62.6, showing a positive correlation with nurse job satisfaction, nurse job esteem, and nursing professional pride. Gender equity positively predicted nurse job esteem. (4) Conclusions: The gender equity in nursing education programs has positive predictive effects on the factors that influence male nurse job satisfaction. In order to increase nurse job retention, educators should incorporate strategies to minimize gender inequity.
Influence of Sports Participation on the Behaviors of Customers of Sports Services: Linear and Qualitative Comparative Analysis Models
Authors: García-Pascual Fernando , Ballester-Esteve Ignacio , Calabuig Ferran
Published: May 2023

Sports services have become an important enclave for our society. Due to its complete sports offer, customers can perform physical activity that helps them to improve their health and well-being. In fitness centers, managers try to know what their customers are looking for in order to create more personalized experiences, as well as to improve their health. This study seeks to understand the influence that sports frequency has on the future behavior of users of a sports center, using two complementary methodologies. A sample of 383 users of a private sports center was used. The two complementary methodologies used were linear models and comparative qualitative analysis, based on the combination of sets. The results show how sports frequency influences the process of creating users’ future behaviors. Considering that none of the variables are necessary, it is observed that perceived value has a significant influence on users’ future behaviors. The use of two complementary methodologies provides a more complete understanding, which helps sports managers to plan and manage effectively to ensure user satisfaction and loyalty. In addition, facilities can incentivize customers through loyalty programs and promotions to maintain their engagement, as well as healthy styles to encourage service recommendations.
Prevalence of Conscientious Objection Policies to Harmful Animal Use in Education at Medical and Veterinary Faculties in Europe
Authors: Zemanova Miriam A.
Published: May 2023

The harmful use of animals in university education has been the topic of an ongoing debate for many years. With growing animal welfare concerns and the advancement of humane teaching methods, students have been asking for more ethical educational approaches. Consequently, many universities have established policies regarding conscientious objection to harmful animal use in education. These policies allow students and faculty members who object to the harmful use of animals on ethical or religious grounds to opt out of participation in such activities without facing negative consequences. Several universities worldwide have already implemented formal policies. However, no studies have yet investigated the extent of conscientious objection policies at universities within the EU and Switzerland. Therefore, the aim of this study was to assess—for the first time—the prevalence of conscientious objection policies at medical and veterinary faculties in Europe. The data showed that 94% of 348 faculties across 28 European countries still do not have a written and publicly available policy that allows students to use humane teaching methods. The future development and widespread implementation of such policies is an essential step toward creating an educational environment that is inclusive, respectful, and committed to ethical and innovative practices.
Effect of EC Levels of Nutrient Solution on Glasswort (Salicornia perennis Mill.) Production in Floating System
Authors: Okudur Esra , Tuzel Yuksel
Published: May 2023

Halophytes have evolved to tolerate high salinity environments. The halophyte glasswort (Salicornia and Sarcocornia species) grows by the sea or in salty soils and can be consumed with pleasure. In this study, the cultivation of glasswort was studied by testing the effects of different electrical conductivity (EC) levels (10, 15, 20, 25, 30 and 35 mS cm−1) of a nutrient solution. Salicornia perennis Mill. was grown on floating systems in unheated greenhouse conditions. To adjust the different EC levels, sodium chloride was added to the Hoagland nutrient solution (EC: 2 mS cm−1). Plant growth and yield parameters, shoot color, evapotranspiration, and shoot nutrient content were determined. Among the tested EC levels, the highest plant height (33.56 cm), shoot (172.75 g) and root fresh weights (41.74 g), stem diameter (7.85 mm), and fresh biomass (2864.06 g m−2) were obtained from an EC level of 25 mS cm−1. There were no significant differences in shoot color excluding b* and chroma values. It was concluded that glasswort could be grown in hydroponic systems as a new crop and that an EC value of 25 mS cm−1 is the most appropriate for the cultivation of Salicornia perennis Mill. on floating systems.
Research and Evaluation in a Child-Focused Place-Based Initiative: West Philly Promise Neighborhood
Authors: Lê-Scherban Félice , Headen Irene , Klem Adena M. , Traister Kelley , Gilliam Erikka , Beverly Maggie , Jannetti Matthew , Ferroni Joanne , Carroll-Scott Amy
Published: May 2023

Place-based initiatives attempt to reduce persistent health inequities through multisectoral, cross-system collaborations incorporating multiple interventions targeted at varying levels from individuals to systems. Evaluations of these initiatives may be thought of as part of the community change process itself with a focus on real-time learning and accountability. We described the design, implementation, challenges, and initial results of an evaluation of the West Philly Promise Neighborhood, which is a comprehensive, child-focused place-based initiative in Philadelphia, Pennsylvania. Priorities for the evaluation were to build processes for and a culture of ongoing data collection, monitoring, and communication, with a focus on transparency, accountability, and data democratization; establish systems to collect data at multiple levels, with a focus on multiple uses of the data and future sustainability; and adhere to grant requirements on data collection and reporting. Data collection activities included the compilation of neighborhood-level indicators; the implementation of a program-tracking system; administrative data linkage; and neighborhood, school, and organizational surveys. Baseline results pointed to existing strengths in the neighborhood, such as the overwhelming majority of caregivers reporting that they read to their young children (86.9%), while other indicators showed areas of need for additional supports and were programmatic focuses for the initiative (e.g., about one-quarter of young children were not engaged in an early childhood education setting). Results were communicated in multiple formats. Challenges included aligning timelines, the measurement of relationship-building and other process-focused outcomes, data and technology limitations, and administrative and legal barriers. Evaluation approaches and funding models that acknowledge the importance of capacity-building processes and allow the development and measurement of population-level outcomes in a realistic timeframe are critical for measuring the success of place-based approaches.
Prevalence of Polycystic Ovary Syndrome amongst Females Aged between 15 and 45 Years at a Major Women’s Hospital in Dubai, United Arab Emirates
Authors: Mirza Fadi G. , Tahlak Muna A. , Hazari Komal , Khamis Amar Hassan , Atiomo William
Published: May 2023

Objective criteria have been scarce in published data on the occurrence of polycystic ovary syndrome (PCOS) in the United Arab Emirates (UAE). It is crucial that we enhance our comprehension of PCOS prevalence in the UAE to inform key stakeholders about the disease’s burden and enable comparisons with other nations. This research aimed to examine the PCOS prevalence at a large academic tertiary centre in Dubai, UAE, called Latifa Women and Children’s Hospital. We performed a cross-sectional study by reviewing the electronic medical records of patients accessing care between 2017 and 2022 (5 years). By utilizing the international classification of diseases codes (ICD-10), we discovered a period prevalence of PCOS of 1.6% among 64,722 women aged between 15 and 45 years. It is worth noting that the estimated annual point prevalence rose from 1.19% in 2020 (at the beginning of the COVID19 pandemic) to 2.72% in 2022 (after the start of the COVID-19 pandemic). Therefore, the odds ratio of the risk of a PCOS diagnosis in 2022 compared to 2020 was 2.28. The majority of the women diagnosed with PCOS in this study had an ICD-10 code of E28.2. Women with PCOS were younger than the controls, less likely to be pregnant, and had a higher body mass index and systolic and diastolic blood pressure. This is the most extensive research to date examining PCOS prevalence in the UAE, and it emphasizes the significance of this condition. It is crucial to prioritize PCOS to prevent morbidity and mortality from reproductive and long-term health consequences, including infertility, type 2 diabetes and endometrial cancer, which is presently the most frequent gynecological cancer in the UAE.
Inequalities in Environmental Cancer Risk and Carcinogen Exposures: A Scoping Review
Authors: Larsen Kristian , Rydz Ela , Peters Cheryl E.
Published: May 2023

Background: Cancer is the leading cause of death in Canada and a major cause of death worldwide. Environmental exposure to carcinogens and environments that may relate to health behaviors are important to examine as they can be modified to lower cancer risks. Built environments include aspects such as transit infrastructure, greenspace, food and tobacco environments, or land use, which may impact how people move, exercise, eat, and live. While environments may play a role in overall cancer risk, exposure to carcinogens or healthier environments is not equitably spread across space. Exposures to carcinogens commonly concentrate among socially and/or economically disadvantaged populations. While many studies have examined inequalities in exposure or cancer risk, this has commonly been for one exposure. Methods: This scoping review collected and synthesized research that examines inequities in carcinogenic environments and exposures. Results: This scoping review found that neighborhoods with higher proportions of low-income residents, racialized people, or same-sex couples had higher exposures to carcinogens and environments that may influence cancer risk. There are currently four main themes in research studying inequitable exposures: air pollution and hazardous substances, tobacco access, food access, and other aspects of the built environment, with most research still focusing on air pollution. Conclusions: More work is needed to understand how exposures to these four areas intersect with other factors to reduce inequities in exposures to support longer-term goals toward cancer prevention.
Effect of Nordic Walking Training on Physical Fitness and Self-Assessment of Health of People with Chronic Non-Specific Lower Back Pain
Authors: Saulicz Mariola , Saulicz Aleksandra , Myśliwiec Andrzej , Knapik Andrzej , Rottermund Jerzy , Saulicz Edward
Published: May 2023

In order to determine the impact of a four-week cycle of Nordic Walking (NW) training on the physical fitness of people with chronic non-specific lower back pain and the impact of this form of activity on their self-assessment of health quality, the study included 80 men and women aged 29 to 63 years. The subjects were divided into two equal (40-person) groups: experimental and control. In both study groups the degree of disability in daily activities caused by back pain was assessed with the FFb-H-R questionnaire, the physical fitness was evaluated with the modified Fullerton test and the sense of health quality was assessed with the SF-36 questionnaire. The same tests were repeated after four weeks. In the experimental group NW training was applied between the two studies. During four weeks, 10 training units were carried out, and each training session lasted 60 min with a two-day break between each training. The four-week NW training resulted in a statistically significant sense of disability due to back pain (p < 0.001), significant improvement of physical fitness expressed by improvement in upper (p < 0.001) and lower (p < 0.01) body strength, upper and lower body flexibility (p < 0.001) and ability to walk a longer distance in a 6-min walk test (p < 0.001). The training participants also showed significant improvements in health quality in both physical (p < 0.001) and mental (p < 0.001) components. The four-week NW training has a positive impact on the physical fitness of men and women with chronic lower back pain. Participation in NW training also contributes to a significant reduction in the sense of disability caused by back pain and improvement in the self-assessment of health quality.
Evaluation of a Childhood Obesity Program Serving a High-Need Population in Brooklyn, New York Using Survival Analysis
Authors: James Alecia , Afable Aimee , Bayoumi Nagla , Dhuper Sarita
Published: May 2023

In this study, we used survival analysis to evaluate whether contact hours intensity was associated with a reduction in time to improvement of various BMI metrics over a 5-year follow-up period at the Live Light Live Right pediatric obesity program in Brooklyn, New York. This was a single-center retrospective longitudinal study of 406 patients during 2010–2016. Participants were categorized based on hours of exposure to Live Light Live Right’s interventions; high contact hours (≥50 h) vs. low contact hours (<50 h). At baseline, 88% of patients in the high contact hour group had severe obesity and the mean age for this group was 10.0 ± 2.66. High contact hours were independently associated with a shorter time to BMI improvement in the sample. There was also a significant association between high contact hours and a longer duration in the improved state. Survival analysis was successful in evaluating the efficacy of the Live Light Live Right Program and demonstrated a positive association between greater intervention intensity and a healthier metabolic profile. Patients’ active engagement in a robust treatment model exemplified by Live Light Live Right is recommended to address the childhood obesity crisis in central Brooklyn.
Prevalence, Correlates, and Sequelae of Child Sexual Abuse (CSA) among Indigenous Canadians: Intersections of Ethnicity, Gender, and Socioeconomic Status
Authors: Helmus L. Maaike , Kyne Ashley
Published: May 2023

Child sexual abuse (CSA) is a severe and concerning public-health problem globally, but some children are at higher risk of experiencing it. The harms caused by colonization and particularly the inter-generational legacy of residential schools would presumably increase the vulnerability of Indigenous children in former British colonies. Among 282 Indigenous participants in Canada recruited from Prime Panels, CSA was reported by 35% of boys, 50% of girls, and 57% of trans and gender non-conforming participants. These rates are substantially higher than global meta-analytic estimates (7.6% of boys and 18.0% of girls). There was evidence of intersectionality based on socioeconomic status. CSA was associated with a variety of other indicators of negative childhood experiences and significantly predicted numerous negative outcomes in adulthood, including mental-health issues (e.g., PTSD), unemployment, and criminal legal-system involvement. Sexual abuse of Indigenous Canadian children is a public-health crisis, and layers of marginalization (e.g., gender, social class) exacerbate this risk. Trauma-informed services to address the harms of colonization are severely needed, in line with recommendations from Canada’s Truth and Reconciliation Commission.
Gait Training with Functional Electrical Stimulation Improves Mobility in People Post-Stroke
Authors: Dantas Maria Tereza Artero Prado , Fernani Deborah Cristina Gonçalves Luiz , Silva Talita Dias da , Assis Iramaia Salomão Alexandre de , Carvalho Augusto Cesinando de , Silva Sidney Benedito , Abreu Luiz Carlos de , Barbieri Fabio Augusto , Monteiro Carlos Bandeira de Mello
Published: May 2023

(1) Background: Stroke is one of the leading causes of disability. To identify the best treatment strategies for people with stroke (PwS), the aim of the current study was to compare the effects of training on a treadmill with functional electrical stimulation (TT-FES) with training on a treadmill (TT), and to analyze the effects of sequence of training on mobility and the parameters of walking ability. (2) Methods: Prospective, longitudinal, randomized and crossover study, in which 28 PwS were distributed into groups, namely the A-B Group (TT-FES followed by TT) and B-A Group (TT followed by TT-FES), using the foot drop stimulator, and were measured with functional tests. (3) Results: We found improved mobility, balance, non-paretic limb coordination, and endurance only in the group that started with TT-FES. However, sensorimotor function improved regardless of the order of training, and paretic limb coordination only improved in the B-A Group, but after TT-FES. These data indicate that the order of the protocols changed the results. (4) Conclusions: Although biomechanical evaluation methods were not used, which can be considered a limitation, our results showed that TT-FES was superior to isolated training on a treadmill with regard to balance, endurance capacity, and coordination of the non-paretic limb.
Towards the Monitoring of Underground Caves Using Geomatics and Geophysical Techniques: 3D Analyses and Seismic Response
Authors: Dabove Paolo , Colombero Chiara , Salerno Quaroni Andrea
Published: May 2023

Analyses of climate change, due to its impact not only on the weather and the environment but also on human health and life, are one of the most important study activities made in recent years. There is relatively high confidence that glacial melt and heavy rainfall events will continue to increase. These climate-related events carry a microseismic signature that can guide monitoring activities. In the last decade, there have been growing applications of long-term continuous ambient seismic noise systems to monitor landslides and potentially unstable rock sites. This work reports some of the activities made during a project performed under the Department of Excellence on Climate Change (2018–2022), funded by the Italian Ministry for University and Research (MUR), in order to improve environmental seismic analyses. The selected test site is the Bossea Cave (NW Italy), where two seismic stations were installed. The goals were to use these stations to understand and study climate change events above the Bossea Cave, analyzing the data from a geophysical and geomatics point of view. Starting with UAV flights and photogrammetric processing to obtain a 3D model of the cave, both ambient seismic noise and microseismicity analyses highlighted an important effect of air temperature and precipitation on the seismic response of the monitored rock mass overlying the Bossea Cave. In particular, a clear effect on the ambient seismic noise spectral content and the peak frequency of the microseismic events driven by temperature and precipitation was found during the warmer monitoring months, with almost zero delays in the seismic response. This is a preliminary but important study, even if longer monitoring data and thermal modeling efforts are needed to fully understand this seasonal variation.
Novel Knowledge about Molecular Mechanisms of Heparin-Induced Thrombocytopenia Type II and Treatment Targets
Authors: Mongirdienė Aušra , Liuizė Agnė , Kašauskas Artūras
Published: May 2023

Heparin-induced thrombocytopenia type II (HIT II), as stated in the literature, occurs in about 3% of all patients and in 0.1–5% of surgical patients. Thrombosis develops in 20–64% of patients with HIT. The mortality rate in HIT II has not decreased using non-heparin treatment with anticoagulants such as argatroban and lepirudin. An improved understanding of the pathophysiology of HIT may help identify targeted therapies to prevent thrombosis without subjecting patients to the risk of intense anticoagulation. The review will summarize the current knowledge about the pathogenesis of HIT II, potential new therapeutic targets related to it, and new treatments being developed. HIT II pathogenesis involves multi-step immune-mediated pathways dependent on the ratio of PF4/heparin and platelet, monocyte, neutrophil, and endothelium activation. For years, only platelets were known to take part in HIT II development. A few years ago, specific receptors and signal-induced pathways in monocytes, neutrophils and endothelium were revealed. It had been shown that the cells that had become active realised different newly formed compounds (platelet-released TF, TNFα, NAP2, CXCL-7, ENA-78, platelet-derived microparticles; monocytes-TF-MPs; neutrophils-NETs), leading to additional cell activation and consequently thrombin generation, resulting in thrombosis. Knowledge about FcγIIa receptors on platelets, monocytes, neutrophils and FcγIIIa on endothelium, chemokine (CXCR-2), and PSGL-1 receptors on neutrophils could allow for the development of a new non-anticoagulant treatment for HIT II. IgG degradation, Syk kinase and NETosis inhibition are in the field of developing new treatment possibilities too. Accordingly, IdeS and DNases-related pathways should be investigated for better understanding of HIT pathogenesis and the possibilities of being the HIT II treatment targets.
BMP-2 Genome-Edited Human MSCs Protect against Cartilage Degeneration via Suppression of IL-34 in Collagen-Induced Arthritis
Authors: Chae Dong-Sik , Han Seongho , Lee Min-Kyung , Kim Sung-Whan
Published: May 2023

Even though the regenerative potential of mesenchymal stem cells (MSCs) has been extensively studied, there is a debate regarding their minimal therapeutic properties. Bone morphogenetic proteins (BMP) are involved in cartilage metabolism, chondrogenesis, and bone healing. In this study, we aimed to analyze the role of genome-edited BMP-2 overexpressing amniotic mesenchymal stem cells (AMMs) in a mouse model of collagen-induced arthritis (CIA). The BMP-2 gene was synthesized and inserted into AMMs using transcription activator-like effector nucleases (TALENs), and BMP-2-overexpressing AMMs (AMM/B) were sorted and characterized using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The co-culture of AMM/B with tumor necrosis factor (TNF)-α-treated synovial fibroblasts significantly decreased the levels of interleukin (IL)-34. The therapeutic properties of AMM/B were evaluated using the CIA mouse model. The injection of AMM/B attenuated CIA progression and inhibited T helper (Th)17 cell activation in CIA mice. In addition, the AMM/B injection increased proteoglycan expression in cartilage and decreased the infiltration of inflammatory cells and factors, including IL-1β, TNF-α, cyclooxygenase (COX)-2, and Nuclear factor kappa B (NF-kB) in the joint tissues. Therefore, editing the BMP-2 genome in MSCs might be an alternative strategy to enhance their therapeutic potential for treating cartilage degeneration in arthritic joints.
CLEC16A—An Emerging Master Regulator of Autoimmunity and Neurodegeneration
Authors: Pandey Rahul , Bakay Marina , Hakonarson Hakon
Published: May 2023

CLEC16A is emerging as an important genetic risk factor for several autoimmune disorders and for Parkinson disease (PD), opening new avenues for translational research and therapeutic development. While the exact role of CLEC16A in health and disease is still being elucidated, the gene plays a critical role in the regulation of autophagy, mitophagy, endocytosis, intracellular trafficking, immune function, and in biological processes such as insulin secretion and others that are important to cellular homeostasis. As shown in both human and animal modeling studies, CLEC16A hypofunction predisposes to both autoinflammatory phenotype and neurodegeneration. While the two are clearly related, further functional studies are needed to fully understand the mechanisms involved for optimized therapeutic interventions. Based on recent data, mitophagy-inducing drugs may be warranted, and such therapy should be tested in clinical trials as these drugs would tackle the underlying pathogenic mechanism (s) and could treat or prevent symptoms of autoimmunity and neurodegeneration in individuals with CLEC16A risk variants. Accordingly, interventions directed at reversing the dysregulated mitophagy and the consequences of loss of function of CLEC16A without activating other detrimental cellular pathways could present an effective therapy. This review presents the emerging role of CLEC16A in health and disease and provides an update on the disease processes that are attributed to variants located in the CLEC16A gene, which are responsible for autoimmune disorders and neurodegeneration with emphasis on how this information is being translated into practical and effective applications in the clinic.
Spatial Attention Modulates Neuronal Interactions between Simple and Complex Cells in V1
Authors: Zheng Zhiyan , Hu Qiyi , Bu Xiangdong , Jiang Hongru , Sui Xiaohong , Li Liming , Chai Xinyu , Chen Yao
Published: May 2023

Visual perception is profoundly modulated by spatial attention, which can selectively prioritize goal-related information. Previous studies found spatial attention facilitated the efficacy of neuronal communication between visual cortices with hierarchical organizations. In the primary visual cortex (V1), there is also a hierarchical connection between simple (S) and complex (C) cells. We wonder whether and how spatial attention modulates neuronal communication within V1, especially for neuronal pairs with heterogeneous visual input. We simultaneously recorded the pairs’ activity from macaque monkeys when they performed a spatial-attention-involved task, then applied likelihood-based Granger causality analysis to explore attentional modulation of neuronal interactions. First, a significant attention-related decrease in Granger causality was found in S-C pairs, which primarily displayed in the S-to-C feedforward connection. Second, the interaction strength of the feedforward connection was significantly higher than that of the feedback under attend toward (AT) conditions. Although information flow did not alter as the attentional focus shifted, the strength of communications between target- and distractor-stimuli-covered neurons differed only when attending to complex cells’ receptive fields (RFs). Furthermore, pairs’ communications depended on the attentional modulation of neurons’ firing rates. Our findings demonstrate spatial attention does not induce specific information flow but rather amplifies directed communication within V1.
Androgen Receptor Imaging in the Management of Hormone-Dependent Cancers with Emphasis on Prostate Cancer
Authors: Kairemo Kalevi , Hodolic Marina
Published: May 2023

Prostate cancer is dependent on the action of steroid hormones on the receptors. Endocrine therapy inhibits hormone production or blocks the receptors, thus providing clinical benefit to many, but not all, oncological patients. It is difficult to predict which patient will benefit from endocrine therapy and which will not. Positron Emission Tomography (PET) imaging of androgen receptors (AR) may provide functional information on the likelihood of endocrine therapy response in individual patients. In this article, we review the utility of [18F]FDHT-PET imaging in prostate, breast, and other hormone-dependent cancers expressing AR. The methodologies, development, and new possibilities are discussed as well.
Intrathecal Injection of Autologous Mesenchymal Stem-Cell-Derived Extracellular Vesicles in Spinal Cord Injury: A Feasibility Study in Pigs
Authors: Shulman Ilya , Ageeva Tatyana , Kostennikov Alexander , Ogurcov Sergei , Tazetdinova Leysan , Kabdesh Ilyas , Rogozhin Alexander , Ganiev Ilnur , Rizvanov Albert , Mukhamedshina Yana
Published: May 2023

Spinal cord injury (SCI) remains one of the current medical and social problems, as it causes deep disability in patients. The use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) is one strategy for stimulating the post-traumatic recovery of the structure and function of the spinal cord. Here, we chose an optimal method for obtaining cytochalasin B-induced EVs, including steps with active vortex mixing for 60 s and subsequent filtration to remove nuclei and disorganized inclusions. The therapeutic potential of repeated intrathecal injection of autologous MSC-derived EVs in the subacute period of pig contused SCI was also evaluated for the first time. In this study, we observed the partial restoration of locomotor activity by stimulating the remyelination of axons and timely reperfusion of nervous tissue.
Role of Sex Hormones in Prevalent Kidney Diseases
Authors: Conte Carolina , Antonelli Giulia , Melica Maria Elena , Tarocchi Mirko , Romagnani Paola , Peired Anna Julie
Published: May 2023

Chronic kidney disease (CKD) is a constantly growing global health burden, with more than 840 million people affected worldwide. CKD presents sex disparities in the pathophysiology of the disease, as well as in the epidemiology, clinical manifestations, and disease progression. Overall, while CKD is more frequent in females, males have a higher risk to progress to end-stage kidney disease. In recent years, numerous studies have highlighted the role of sex hormones in the health and diseases of several organs, including the kidney. In this review, we present a clinical overview of the sex-differences in CKD and a selection of prominent kidney diseases causing CKD: lupus nephritis, diabetic kidney disease, IgA nephropathy, and autosomal dominant polycystic kidney disease. We report clinical and experimental findings on the role of sex hormones in the development of the disease and its progression to end-stage kidney disease.
The Role of Paxillin Aberrant Expression in Cancer and Its Potential as a Target for Cancer Therapy
Authors: Liu Weixian , Huang Xinxian , Luo Weizhao , Liu Xinguang , Chen Weichun
Published: May 2023

Paxillin is a multi-domain adaptor protein. As an important member of focal adhesion (FA) and a participant in regulating cell movement, paxillin plays an important role in physiological processes such as nervous system development, embryonic development, and vascular development. However, increasing evidence suggests that paxillin is aberrantly expressed in many cancers. Many scholars have also recognized that the abnormal expression of paxillin is related to the prognosis, metastases, invasion, survival, angiogenesis, and other aspects of malignant tumors, suggesting that paxillin may be a potential cancer therapeutic target. Therefore, the study of how aberrant paxillin expression affects the process of tumorigenesis and metastasis will help to develop more efficacious antitumor drugs. Herein, we review the structure of paxillin and its function and expression in tumors, paying special attention to the multifaceted effects of paxillin on tumors, the mechanism of tumorigenesis and progression, and its potential role in tumor therapy. We also hope to provide a reference for the clinical prognosis and development of new tumor therapeutic targets.
In Vitro Skeletal Muscle Model of PGM1 Deficiency Reveals Altered Energy Homeostasis
Authors: Conte Federica , Ashikov Angel , Mijdam Rachel , van de Ven Eline G. P. , van Scherpenzeel Monique , Veizaj Raisa , Mahalleh-Yousefi Seyed P. , Post Merel A. , Huijben Karin , Panneman Daan M. , Rodenburg Richard J. T. , Voermans Nicol C. , Garanto Alejandro , Koopman Werner J. H. , Wessels Hans J. C. T. , Noga Marek J. , Lefeber Dirk J.
Published: May 2023

Phosphoglucomutase 1 (PGM1) is a key enzyme for the regulation of energy metabolism from glycogen and glycolysis, as it catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate. PGM1 deficiency is an autosomal recessive disorder characterized by a highly heterogenous clinical spectrum, including hypoglycemia, cleft palate, liver dysfunction, growth delay, exercise intolerance, and dilated cardiomyopathy. Abnormal protein glycosylation has been observed in this disease. Oral supplementation with D-galactose efficiently restores protein glycosylation by replenishing the lacking pool of UDP-galactose, and rescues some symptoms, such as hypoglycemia, hepatopathy, and growth delay. However, D-galactose effects on skeletal muscle and heart symptoms remain unclear. In this study, we established an in vitro muscle model for PGM1 deficiency to investigate the role of PGM1 and the effect of D-galactose on nucleotide sugars and energy metabolism. Genome-editing of C2C12 myoblasts via CRISPR/Cas9 resulted in Pgm1 (mouse homologue of human PGM1, according to updated nomenclature) knockout clones, which showed impaired maturation to myotubes. No difference was found for steady-state levels of nucleotide sugars, while dynamic flux analysis based on 13C6-galactose suggested a block in the use of galactose for energy production in knockout myoblasts. Subsequent analyses revealed a lower basal respiration and mitochondrial ATP production capacity in the knockout myoblasts and myotubes, which were not restored by D-galactose. In conclusion, an in vitro mouse muscle cell model has been established to study the muscle-specific metabolic mechanisms in PGM1 deficiency, which suggested that galactose was unable to restore the reduced energy production capacity.
PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation
Authors: Solovieva Anastasiya O. , Sitnikova Natalia A. , Nimaev Vadim V. , Koroleva Elena A. , Manakhov Anton M.
Published: May 2023

Diabetic foot ulcers (DFU) are a common complication of Type 2 Diabetes Mellitus (T2DM). Development of bioactive wound healing covers is an important task in medicine. The use of autologous platelet-rich plasma (PRP) consisting of growth factors, cytokines and components of extracellular matrix is a perspective approach for DFU treatment, but we previously found that some T2DM PRP samples have a toxic effect on mesenchymal stem cells (MSCs) in vitro. Here, we covalently immobilized T2DM PRP proteins on polycaprolactone (PCL) nanofibers, and the growth of endothelial cells on the PCL-COOH-PRP was investigated. Additionally, the level of NO reflecting the cytotoxic effects of PRP, angiogenin, and VEGF levels were measured in T2DM PRP samples. The results showed that the application of PCL-COOH-PRP nanofibers allows to remove the cytotoxicity of T2DM PRP and to improve endothelial cell adhesion and proliferative activity. We showed that the origin of T2DM PRP (the level of PRP toxicity or presence/absence of DFU) does not influence the efficiency of cell growth on PCL-COOH-PRP, and on the level of angiogenin, vascular epidermal growth factor (VEGF) in PRP itself.