bluuebunny/crossref_metadata_2025 huggingface dataset:
A case report of robotic-assisted resection of large fibrous benign tumor of second rib
Authors: Liu Bohao , Gao Shan , Wu Qifei , Li Haijun , Zhang Guangjian , Fu Junke
Published: December 2022

Background

Surgical resection is the most effective curative management of benign rib tumors and carries an excellent prognosis. Due to complex anatomy and narrow field, higher rib resection is technically demanding and requires extensive dissection.

Case presentation

We report a case of second rib tumor resection performed transthoracic under Da Vinci robot assistance. A 32-year-old male complained about increasing pain in the left anterior chest wall. After 3D reconstruction of CT, it showed a well-circumscribed fusiform lesion with a multi-component structure. Measured 17 × 6 × 4 cm and extended into the chest cavity to the depth below the pectoralis minor muscle. The patient underwent robotic-assisted trans-thoracic second rib resection. At four weeks of outpatient follow-up, the patient reported no pain and uncomplicated wound healing.

Conclusion

This minimally invasive approach offers optimal visualization and tissue manipulation while dramatically decreasing the possibility of collateral damage, hence ensuring fast function recovery. To the best of our knowledge, these kinds of procedures are rarely reported in detail.
Prenatal diagnosis and molecular cytogenetic analyses of a paternal inherited deletion of 1q23.3 encompassing PBX1 gene
Authors: Luo Man , Gu Xia , Zhou Ting , Chen Chaoli
Published: December 2022

Background

Patients with deletions involving the long arm of chromosome 1 are rare. The PBX1 gene is located on chromosome 1q23.3. PBX1 encodes a transcription factor which promotes protein–protein interaction and plays a crucial role in several developmental processes. PBX1 haploinsufficiency had been reported to lead syndromic congenital anomalies of kidney and urinary tract (CAKUT) in humans.

Case presentation

In this research, a 24-year-old woman (gravida 1, para 0) underwent amniocentesis at 22 weeks’ gestation because of a horseshoe kidney of the fetus on prenatal ultrasound.

Results

Chromosomal microarray analysis (CMA) from this family revealed a 1.14 Mb paternal inherited deletion on chromosome 1q23.3, spanning from position 163,620,000 to 164,760,000 (hg19). Trio whole-exome sequencing (WES) showed heterozygous deletions in exons 1–2 of the PBX1 in fetal and paternal samples. At the 3-year follow-up, the baby did not have an abnormal phenotype except a horseshoe kidney.

Conclusion

We provide a detailed description of the phenotype in a family with paternal inherited deletion of 1q23.3 encompassing exons 1–2 of the PBX1 gene. Combination of karyotype analysis, CMA, WES, prenatal ultrasound and genetic counseling is helpful for the prenatal diagnosis of chromosomal microdeletions/microduplications.
The effect of targeted rheumatoid arthritis therapeutics on systemic inflammation and anemia: analysis of data from the CorEvitas RA registry
Authors: Padula Anthony S. , Pappas Dimitrios A. , Fiore Stefano , Blachley Taylor S. , Ford Kerri , Emeanuru Kelechi , Kremer Joel M.
Published: December 2022

Background

To evaluate the effects of tumor necrosis factor inhibitors (TNFi), interleukin-6 receptor inhibitors (IL-6Ri), and Janus kinase inhibitors (JAKi) on hemoglobin (Hb) and C-reactive protein (CRP) levels in adults enrolled in CorEvitas (formerly Corrona), a large US rheumatoid arthritis (RA) registry.

Methods

Patients who initiated TNFi, IL-6Ri, or JAKi treatment during or after January 2010, had Hb and CRP measurements at baseline and 6-month follow-up (± 3 months) and had continued therapy at least until that follow-up, through March 2020, were included in the analysis. Changes in Hb and CRP were assessed at month 6. Abnormal Hb was defined as < 12 g/dL (women) or < 13 g/dL (men); abnormal CRP was ≥ 0.8 mg/dL. Differences in Hb and CRP levels were evaluated using multivariable regression.

Results

Of 2772 patients (TNFi, 65%; IL-6Ri, 17%; JAKi, 17%) evaluated, 1044 (38%) had abnormal Hb or CRP at initiation; an additional 252 (9%) had both abnormal Hb and CRP. At month 6, the IL-6Ri group had a greater Hb increase than the TNFi (mean difference in effect on Hb: 0.28 g/dL; 95% CI 0.19–0.38) and JAKi (mean difference in effect on Hb: 0.47 g/dL; 95% CI 0.35–0.58) groups, regardless of baseline Hb status (both p < 0.001). The CRP decrease at month 6 was greater with IL-6Ri compared with TNFi and JAKi, regardless of baseline CRP status (both p < 0.05).

Conclusion

These real-world results align with the mechanism of IL-6R inhibition and may inform treatment decisions for patients with RA.
Lecanemab in patients with early Alzheimer’s disease: detailed results on biomarker, cognitive, and clinical effects from the randomized and open-label extension of the phase 2 proof-of-concept study
Authors: McDade Eric , Cummings Jeffrey L. , Dhadda Shobha , Swanson Chad J. , Reyderman Larisa , Kanekiyo Michio , Koyama Akihiko , Irizarry Michael , Kramer Lynn D. , Bateman Randall J.
Published: December 2022

Background

Lecanemab, a humanized IgG1 monoclonal antibody that targets soluble aggregated Aβ species (protofibrils), has demonstrated robust brain fibrillar amyloid reduction and slowing of clinical decline in early AD. The objective of this analysis is to report results from study 201 blinded period (core), the open-label extension (OLE), and gap period (between core and OLE) supporting the effectiveness of lecanemab.

Methods

The lecanemab study 201 core was a double-blind, randomized, placebo-controlled study of 856 patients randomized to one of five dose regimens or placebo. An OLE of study 201 was initiated to allow patients to receive open-label lecanemab 10mg/kg biweekly for up to 24 months, with an intervening off-treatment period (gap period) ranging from 9 to 59 months (mean 24 months).

Results

At 12 and 18 months of treatment in the core, lecanemab 10 mg/kg biweekly demonstrated dose-dependent reductions of brain amyloid measured PET and corresponding changes in plasma biomarkers and slowing of cognitive decline. The rates of clinical progression during the gap were similar in lecanemab and placebo subjects, with clinical treatment differences maintained after discontinued dosing over an average of 24 months in the gap period. During the gap, plasma Aβ42/40 ratio and p-tau181 levels began to return towards pre-randomization levels more quickly than amyloid PET. At OLE baseline, treatment differences vs placebo at 18 months in the randomized period were maintained across 3 clinical assessments. In the OLE, lecanemab 10 mg/kg biweekly treatment produced dose-dependent reductions in amyloid PET SUVr, improvements in plasma Aβ42/40 ratio, and reductions in plasma p-tau181.

Conclusions

Lecanemab treatment resulted in significant reduction in amyloid plaques and a slowing of clinical decline. Data indicate that rapid and pronounced amyloid reduction correlates with clinical benefit and potential disease-modifying effects, as well as the potential to use plasma biomarkers to monitor for lecanemab treatment effects.

Trial registration

ClinicalTrials.gov NCT01767311.
Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice
Authors: Xie Huazhong , Qiang Pengfei , Wang Yao , Xia Fan , Liu Peiqing , Li Min
Published: December 2022

Cysteine protease ATG4B, a key autophagy protein, is an attractive target for colorectal cancer therapy. However, ATG4B inhibitors with higher efficiency, safety, and clear mechanism are still limited. In this study, we discovered ATG4B inhibitors based on the FDA-approved drug library through FRET-based high-throughput screening and gel-based analysis. Among the nine hits, compound Ebselen showed the most potent ATG4B inhibitory activity (IC50 = 189 nM) and exhibited controllable selectivity and structural optimizable possibility against ATG4A and caspases. We then performed mass spectrometry assay and cysteine mutations to confirm that Ebselen could covalently bind to ATG4B at Cys74. Moreover, Cys292 and Cys361 instead of Cys74 are responsible for the redox-oligomerization and efficient activity inhibition of ATG4B. Ultimately through cell culture and mouse xenograft tumor models, we established the impact of Ebselen on autophagy and tumor suppression via ATG4B inhibition other than apoptosis. These results suggest that old drug Ebselen as an ATG4B inhibitor through oxidative modification may be repurposed as a promising anti-colorectal cancer drug.
The prognostic effect of ST-elevation in lead aVR on coronary artery disease, and outcome in acute coronary syndrome patients: a systematic review and meta-analysis
Authors: Kazemi Erfan , Mansoursamaei Ali , Bijan Morteza , Hosseinzadeh Ali , Sheibani Hossein
Published: December 2022

Background

Rapid diagnosis of coronary artery disease has an important role in saving patients. The aim of this study is to evaluate if aVR lead ST-elevation (STE) can predict LM/3VD, left main (LM) disease, and three-vessel disease (3VD), outcome in acute coronary syndrome (ACS) patients.

Methods

In this systematic review and meta-analysis, 45 qualified studies were entered. Scopus, Pub med, Google scholar, Web of science, Cochrane library were searched on 12 November 2021.

Results

This systematic review includes 52,175 participants. In patients with STE, the total odds ratios for LM, 3VD, and LM/3VD were 5.48 (95% CI 3.88, 7.76), 2.21 (95% CI 1.78, 3.27), and 6.21 (95% CI 3.49, 11,6), respectively. STE in lead aVR was linked with in-hospital death (OR = 2.99, CI 1.90, 4.72) and 90-day mortality (OR = 3.09, CI 2.17, 4.39), despite the fact that it could not predict 30-day mortality (OR = 1.11, CI 0.95, 1.31). The STE > 1 mm subgroup had the highest sensitivity for LM (0.9, 95% CI 0.82, 0.98), whereas the STE > 0.5 mm (0.76, 95% CI 0.61, 0.90) subgroup had the highest sensitivity for LM/3VD. The appropriate cut-off point with highest specificity for LM/3VD and LM was STE > 1.5 mm (0.80, 95% CI 0.75, 0.85) and STE > 0.5 mm, respectively (0.75, 95% CI 0.67, 0.84, I2 = 97%).

Conclusion

The odds of LM and LM/3VD were higher than 3VD in ACS patients with STE in lead aVR. Also, STE > 0.5 mm was the best cut-off point to screen LM/3VD, whereas for LM diagnosis, STE > 1 mm had the highest sensitivity. Furthermore, LM/3VD had a higher overall specificity than LM.
A high detail UAS-based 3D model of the Torre Benzalá in Jaén, Spain
Authors: Lerma-Cobo F. , Romero-Manchado A. , Enríquez C. , Ramos M. I.
Published: December 2022

The constant development of geomatics tools has driven the opening of their applications to multiple disciplines, including archaeology. The possibility of performing a 3D reconstruction of archaeological remains as well as a semantic classification of the 3D surface facilitates not only a better knowledge of the historical heritage but also an essential aid to the planning and development of restoration and preservation projects of this legacy. Different data exploitation strategies are needed to take advantage of the geospatial data provided by geomatics tools. In this paper, we have studied the current state of conservation of a medieval tower, Torre Benzalá in Jaén, southern Spain. The interesting thing about this study is that very high resolution RGB images, taken by a drone, have been used in order to show the current degree of deterioration of the tower, providing accurate and precise documentation of the current state. Thus, a highly detailed 3D reconstruction of the tower has been carried out. A dense point cloud was generated to obtain a digital elevation model (DEM) to identify and quantify the most critically deteriorated areas. The results are useful for the development of an architectural maintenance and restoration project to preserve this archaeological legacy.
Spectroscopic analysis of mushrooms by surface-enhanced Raman scattering (SERS)
Authors: Puliga Federico , Zuffi Veronica , Zambonelli Alessandra , Francioso Ornella , Sanchez-Cortes Santiago
Published: December 2022

Background

Mushrooms have always been considered an important source of food and biologically active compounds with several medicinal properties. In recent years, different methods were used to study the quality and chemical composition of mushrooms. Among these, Fourier transform infrared (FT-IR) and FT-Raman spectroscopy techniques have been successfully applied to identify different mushroom species. However, the structural biomolecule components existing in the mycelium or in the fruiting bodies may produce strong fluorescence emission that overlaps the Raman radiation, thus avoiding their analyses by Raman. SERS spectroscopy is a powerful technique which uses metal nanoparticles (NPs) to enhance the Raman signal of molecules adsorbed on the NPs surface. In addition, SERS is able to quench the macromolecule florescence. In this work, we have employed silver nanoparticles in order to get mushroom fingerprints based on SERS as quick procedure to analyze and identify different chemical compounds from the fruiting bodies of six edible and/or medicinal mushrooms: Lentinula edodes, Ganoderma lucidum, Pleurotus cornucopiae, Pleurotus ostreatus, Tuber aestivum and Tuber magnatum.

Results

SERS analyses performed directly on fruiting body fragments produced characteristic spectra for each species. One group of mushrooms (L. edodes, G. lucidum, T. aestivum and T. magnatum) was dominated by the bands of nucleic acids; and the other one (P. cornucopiae and P. ostreatus), by the bands of pigments such as melanins; carotenoids; azafilones; polyketides; and flavonoids located in the cell wall. Additionally, bands corresponding to cell wall polysaccharides, particularly chitosan and 1,3-β D-glucan, were identified in the extracts of P. cornucopiae, P. ostreatus and L. edodes. No signal of cell wall polysaccharides was found in G. lucidum extract. Raman mapping of the analyzed samples was useful in tracking the spatial distribution of the marker bands. Moreover, the principal component analysis (PCA) carried out on the acquired SERS spectra, allows to discriminate the analyzed mushroom species.

Conclusions

The SERS technique has the ability to generate a strong Raman signal from mushroom fruiting bodies using Ag-NPs deposited directly on intact, untreated mushroom tissues. Using this methodology, commonly applied laboratory time-consuming methods can be avoided or bypassed as well as analysis time can be reduced.

Graphical Abstract
Microbiome and metagenomic analysis of Lake Hillier Australia reveals pigment-rich polyextremophiles and wide-ranging metabolic adaptations
Authors: Sierra Maria A. , Ryon Krista A. , Tierney Braden T. , Foox Jonathan , Bhattacharya Chandrima , Afshin Evan , Butler Daniel , Green Stefan J. , Thomas W. Kelley , Ramsdell Jordan , Bivens Nathan J. , McGrath Ken , Mason Christopher E. , Tighe Scott W.
Published: December 2022

Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional features, and the prevalence of pigment-producing-metabolisms has not been previously studied. Through metagenomic sequencing and culture-based approaches, our results evidence that Lake Hillier is composed of a diverse set of microorganisms including archaea, bacteria, algae, and viruses. Our data indicate that the microbiome in Lake Hillier is composed of multiple pigment-producer microbes, including Dunaliella, Salinibacter, Halobacillus, Psychroflexus, Halorubrum, many of which are cataloged as polyextremophiles. Additionally, we estimated the diversity of metabolic pathways in the lake and determined that many of these are related to pigment production. We reconstructed complete or partial genomes for 21 discrete bacteria (N = 14) and archaea (N = 7), only 2 of which could be taxonomically annotated to previously observed species. Our findings provide the first metagenomic study to decipher the source of the pink color of Australia’s Lake Hillier. The study of this pink hypersaline environment is evidence of a microbial consortium of pigment producers, a repertoire of polyextremophiles, a core microbiome and potentially novel species.
Reduced restenosis and enhanced re-endothelialization of functional biodegradable vascular scaffolds by everolimus and magnesium hydroxide
Authors: Baek Seung-Woon , Kim Da-Seul , Song Duck Hyun , Kim Han Byul , Lee Semi , Kim Jun Hyuk , Lee Jun-Kyu , Hong Young Joon , Park Chun Gwon , Han Dong Keun
Published: December 2022

Background

Coronary artery disease is a cardiovascular disease with a high mortality and mortality rate in modern society. Vascular stent insertion to restore blood flow is essential to treat this disease. A fully biodegradable vascular scaffold (BVS) is a vascular poly (L-lactic acid) (PLLA) stent that is receiving growing interest as this is biodegradable in the body and does not require secondary removal surgery. However, acidic byproducts composed of PLLA produced during the biodegradation of the BVS can induce an inflammatory response. Magnesium hydroxide, a basic inorganic particle, neutralizes the acidic byproducts of PLLA. 

Methods

In this study, we investigated using a BVS coated with everolimus and surface-modified magnesium hydroxide that suppresses smooth muscle cell proliferation and protects endothelial cells, respectively. The various characteristics of the functional stent were evaluated using in vitro and in vivo analyses. 

Results

The BVS was successfully prepared with evenly coated everolimus and surface-modified magnesium hydroxide. A neutral pH value was maintained by magnesium hydroxide during degradation, and everolimus was released for one month. The coated BVS effectively inhibited protein adsorption and platelet adhesion, demonstrating excellent blood compatibility. In vitro analysis showed that BVS protects endothelial cells with magnesium hydroxide and selectively inhibits smooth muscle cell proliferation via everolimus treatment. The functional BVS was inserted into porcine coronary arteries for 28 days, and the results demonstrated that the restenosis and inflammation greatly decreased and re-endothelialization was enhanced as compared to others.

Conclusions

This study provides new insights into the design of drug-incorporated BVS stent for coronary artery disease.

Graphical Abstract
Formulation of a kit under Good Manufacturing Practices (GMP) for preparing [111In]In-BnDTPA-trastuzumab-NLS injection: a theranostic agent for imaging and Meitner-Auger Electron (MAE) radioimmunotherapy of HER2-positive breast cancer
Authors: Chan Conrad , Prozzo Vanessa , Aghevlian Sadaf , Reilly Raymond M.
Published: December 2022

Background

111In[In]-BnDTPA-trastuzumab-NLS is a radiopharmaceutical with theranostic applications for imaging and Meitner-Auger electron (MAE) radioimmunotherapy (RIT) of HER2-positive breast cancer (BC). Nuclear localization sequence (NLS) peptides route the radiopharmaceutical to the nucleus of HER2-positive BC cells following receptor-mediated internalization for RIT with subcellular range MAEs. The γ-photons emitted by 111In permit tumour imaging by SPECT. Our aim was to formulate a kit under Good Manufacturing Practices conditions to prepare 111In[In]-BnDTPA-trastuzumab-NLS injection for a first-in-human clinical trial.

Results

Trastuzumab was derivatized with p-SCN-BnDTPA to introduce Bn-DTPA for complexing 111In, then modified with maleimide groups for conjugation to the thiol on cysteine in NLS peptides [CGYGPKKKRKVGG]. BnDTPA-trastuzumab-NLS (5 mg in 1.0 mL of 0.05 M ammonium acetate buffer, pH 5.5) was dispensed into unit dose sterile glass vials to produce kits for labeling with 100–165 MBq of 111In[In]Cl3. The kits met specifications for protein concentration (4.5–5.5 mg/mL), volume (0.95–1.05 mL), pH (5.5–6.0), appearance (clear, pale-yellow, particulate-free), BnDTPA substitution level (2.0–7.0 BnDTPA/trastuzumab), purity and homogeneity (SDS-PAGE and SE-HPLC), 111In labeling efficiency (> 90%), binding to HER2-positive SK-BR-3 human breast cancer cells (Ka = 1–8 × 108 L/mmol; Bmax = 0.5–2 × 106 sites/cell), NLS peptide conjugation (upward band shift on SDS-PAGE), sterility (USP Sterility Test) and endotoxins (USP Bacterial Endotoxins Test). 111In-BnDTPA-trastuzumab-NLS injection met specifications for pH (5.5–6.5), radiochemical purity (≥ 90%), radionuclide purity (≥ 99%), appearance (clear, colourless, particle-free) and sterility (retrospective USP Sterility Test). Kits were stable stored at 2–8 °C for up to 661 days (d) meeting all key specifications. Protein concentration remained within or just slightly greater than the specification for up to 139 d. 111In[In]-BnDTPA-trastuzumab-NLS injection was stable for up to 24 h. An expiry of 180 d was assigned for the kits and 8 h for the final radiopharmaceutical.

Conclusion

A kit was formulated under GMP conditions for preparing 111In[In]-BnDTPA-trastuzumab-NLS injection. This radiopharmaceutical was safely administered to 4 patients with HER2-positive BC to trace the uptake of trastuzumab into brain metastases before and after MRI-guided focused ultrasound (MRIg-FUS) by SPECT imaging.
Seroprevalence of hepatitis B and C virus among highly active antiretroviral therapy experienced children in Gondar, Ethiopia
Authors: Kasew Desie , Wondmagegn Mitikie , Bayleyegn Biruk
Published: December 2022

Background

Globally, chronic viral hepatitis is the cause of mortality alongside human immunodeficiency virus/acquired immunodeficiency syndrome and tuberculosis. Published reports on the seroprevalence of hepatitis B and C viruses among HIV-infected children are lacking in sub-Saharan Africa. Hence, this study aimed to determine the seroprevalence of hepatitis B and C viruses among highly active antiretroviral therapy (HAART)-experienced children at the University of Gondar Comprehensive Specialized Hospital.

Methods

We conducted a hospital-based cross-sectional study to determine the seroprevalence of hepatitis B and C viruses among HAART-experienced children from January to May 2020. We collected the socio-demographic characteristics of study participants with pretested questioners and clinical data from medical records. We performed enzyme-linked immunosorbent assay-based laboratory test for serum hepatitis B surface antigens and anti-hepatitis C virus antibodies. Finally, we analyzed the frequency of all variables, determined the association of independent variables with hepatitis B and C viruses by using univariable and then multivariable logistic regression.

Results

A total of 241 HAART-experienced children were enrolled, 49.8% of whom were girls. The median age of participants was 13 years (interquartile range 11–14). The seroprevalence of hepatitis B and C virus infection among HAART-experienced children were 9.5% and 2.9%, respectively. Being underweight was significantly associated with both hepatitis B virus (AOR = 3.87: 95% CI; 1.04–14.46, P = 0.044) and hepatitis C virus infections (AOR = 4.54: 95% CI; 1.21–17.04, P = 0.025).

Conclusions

This study showed that the magnitude of hepatitis B and C viruses was high among HIV-infected children who were under HAART and did not know their hepatitis B and C infection status before. Being underweight was associated with both hepatitis viruses. Therefore, screening for hepatitis B and C viruses should be a routine measure for all HIV-infected children.
Association between Rheumatic Disease Comorbidity Index and factors of poor prognosis in a cohort of 280 patients with rheumatoid arthritis
Authors: Ben Tekaya Aicha , Hannech Emna , Saidane Olfa , Rouached Leila , Bouden Selma , Tekaya Rawdha , Mahmoud Ines , Abdelmoula Leila
Published: December 2022

Background

Rheumatoid arthritis (RA) is commonly associated with higher rates of comorbidities. Recent recommendations highlight screening comorbidities during the disease course because of their impact on patients’ ability to function, on disease outcome, but also on treatment choices. Hence the interest of our study that aimed to quantify the impact of comorbidities among RA patients using a validated tool the Rheumatic Disease Comorbidity Index (RDCI) and to explore the association between comorbidities and disease characteristics.

Methods

We conducted a cross-sectional study over 12 months period, including patients followed for an established RA according to the ACR/EULAR 2010 criteria and hospitalized in our rheumatology department. Patients’ characteristics and disease features were collected for each patient. Comorbidities were quantified using the RDCI. We looked for the association between RDCI and patients characteristics and RA parameters. Univariable and multivariable analysis were made.

Results

They were 280 patients: 233 female (83.2%) and 47 male (16.8%) with a mean age of 58.07 (SD 11.12) years. The mean follow-up period was 14.74 (SD 1.63) years. Comorbidities were noted in 133 patients (47.5%). The mean comorbidity score measured by the RDCI was 1.05 (SD 1.23). RDCI was positively correlated with age (p < 0.001, r = 0.359). RA patients whose age of disease onset exceeds 40 years have significantly higher RDCI (1.8 (SD 1.3) [CI 95%: 1.36–1.88] vs. 1.5 (SD 1.2), p = 0.007). Moreover, RDCI was significantly associated with the presence pulmonary involvement (p < 0.001) and ocular involvement (p = 0.002). RDCI was also associated with erosive RA (p = 0.006), the presence of atlanto-axial dislocation (p = 0.014), and coxitis (p = 0.029). Regarding therapy regimen, RDCI was statistically increased in patients receiving bDMARDs compared to patients under csDMARDs (2.8 (SD 1.6) vs. 1.0 (SD 1.0), p = 0.021).

Conclusion

In this study, comorbidity index was associated with signs of poor prognosis such as erosions, coxitis, and atlanto-axial dislocation. This confirmed the hypothesis that comorbidity can be a threat to the improvement in the long-term prognosis in RA patients.
Can we benefit from game engines to develop digital twins for planning the deployment of photovoltaics?
Authors: Clausen Christian Skafte Beck , Ma Zheng Grace , Jørgensen Bo Nørregaard
Published: December 2022

Digital Twins (DTs) have attracted great attention in the energy sector. Game engines have been suggested to model DTs of their physical counterparts because they provide realistic graphics, lighting-, fluid- and physics engines that simulate the real world. However, the application of game engines to develop DTs for photovoltaics (PVs) has not yet been discussed in the literature. Therefore, this paper assesses the built-in game engine features' ability to support the DT development of PVs with Unreal Engine 5. This paper mainly focuses on visual representation because the surrounding environment significantly impacts PV deployment, and the existing software tools do not allow the study of the environmental factors at the early planning phase of a project’s lifecycle. Furthermore, this paper investigates the position of the sun, shadows and reflections from nearby objects that influence the PVs' power output, and if the built-in light engine can be used for planning the deployment of PVs. The result shows that in-game objects in the environment can be used to affect the simulated PV output estimate over a year. It also indicates that applying Unreal Engine 5 to model PV systems that rely on mirroring real-world behaviour is promising if accurate data is used in the modelling. Real data and mathematical PV models are necessary since Unreal Engine 5’s Lumen subsystem cannot provide realistic solar radiance on PVs for a given location on earth.
Probabilistic Flexoffers in residential heat pumps considering uncertain weather forecast
Authors: Albano Michele , Cibin Nicola , Golmohamadi Hessam , Skou Arne
Published: December 2022

The penetration of renewable energies is increasing in energy systems worldwide. Consequently, the intermittency of the energy sources raises technical challenges for sustainable energy supply. Demand-side flexibility is an effective solution to counterbalance renewable power fluctuations. In the residential sector, electrical heat pumps exhibit great flexibility potential. In this paper, a novel approach is proposed to generate FlexOffers for individual heat pumps considering the uncertain nature of weather conditions. To achieve the aim, firstly, the thermal dynamic model of residential buildings is presented mathematically. The model addresses different temperature zones. The constant coefficients of the thermal dynamics are estimated using Continuous-Time Stochastic Model (CTSM) in R software. Afterward, the building model is integrated with a success function to generate FlexOffers. The success function is comprised of two objective functions including minimization and maximization of the energy consumption of heat pumps. The FlexOffers are generated considering the existing gap between the minimum and maximum energy consumption. The patterns of energy consumption are defined based on occupants’ thermal comfort temperature setpoint. The FlexOffers are programmed in UPPAAL-STRATEGO software. Finally, a high-fidelity building model with four rooms is used to examine the proficiency of the suggested approaches. The simulation results confirm that the proposed method generates flexibility potentials for the upstream network in both optimistic and pessimistic states of energy consumption patterns.
Potentials of game engines for wind power digital twin development: an investigation of the Unreal Engine
Authors: Sørensen Jonas Vedsted , Ma Zheng , Jørgensen Bo Nørregaard
Published: December 2022

Digital twin technologies have become popular in wind energy for monitoring and what-if scenario investigation. However, developing a digital representation of the wind is challenging, especially due to the digital twin platform constraints. Game engines might be possible to solve this issue, especially since game engines have been used for product design, testing, prototyping, and also digital twins. Therefore, this study investigates the potential of developing a digital twin of wind power in the Unreal game engine. A case study of two types of wind turbines (Vestas V164-8 and Enercon E-126 7.580) and one location (Esbjerg, Denmark) is chosen for this study. The digital twin includes the environment with historical wind data and the visual representation of the wind turbine with a wind power production model and the estimated production in the given wind conditions of the area. The results show that game engines are viable for building entire digital twins where a realistic graphical user interface is required. Unreal Engine 5 provides the tools for modelling the landscape, surrounding water, and lighting. In addition, the Unreal Engine ecosystem provides vast amounts of content, such as 3D assets and game logic plugins, easing the digital twin development. The results prove that digital twins built in Unreal Engine 5 have great potential development of digital twins and user interfaces for communicating with a digital twin. The developed digital twin allows for further extension to benefit future digital twins utilizing wind turbines.
Peer-to-peer energy trading optimization in energy communities using multi-agent deep reinforcement learning
Authors: Pereira Helder , Gomes Luis , Vale Zita
Published: December 2022

In the past decade, the global distribution of energy resources has expanded significantly. The increasing number of prosumers creates the prospect for a more decentralized and accessible energy market, where the peer-to-peer energy trading paradigm emerges. This paper proposes a methodology to optimize the participation in peer-to-peer markets based on the double-auction trading mechanism. This novel methodology is based on two reinforcement learning algorithms, used separately, to optimize the amount of energy to be transacted and the price to pay/charge for the purchase/sale of energy. The proposed methodology uses a competitive approach, and that is why all agents seek the best result for themselves, which in this case means reducing as much as possible the costs related to the purchase of energy, or if we are talking about sellers, maximizing profits. The proposed methodology was integrated into an agent-based ecosystem where there is a direct connection with agents, thus allowing application to real contexts in a more efficient way. To test the methodology, a case study was carried out in an energy community of 50 players, where each of the proposed models were used in 20 different players, and 10 were left without training. The players with training managed, over the course of a week, to save 44.65 EUR when compared to a week of peer-to-peer without training, a positive result, while the players who were left without training increased costs by 17.07 EUR.
Framework for dimensioning battery energy storage systems with applied multi-tasking strategies in microgrids
Authors: Nielsen Troels S. , Thomsen Jens S. P. , Santos Athila Q. , Kaad Jutte
Published: December 2022

The shifting from the traditional centralized electric sector to a distributed and renewable system presents some challenges. Battery energy storage technologies have proven effective in relieving some aspects of this transition by facilitating load control and providing flexibility to non-dispatchable renewable production. Therefore, this paper investigates how to dimension battery energy storage systems with applied multi-tasking strategies in microgrids. To this end, it proposes a framework to accurately depict how BESS can be financially and technically feasible by deploying multi-tasking strategies that fit the system characteristics of a microgrid while providing arguments for the financial incentive. The framework development is based on the principles of the analytical approach and is conceptualized in a three-part funnel structure. This framework has been tested using the case study of Aeroe microgrid and resulted in a proposed battery energy storage configuration. Based on the findings, the BESS implementation contributes to improve load behavior and to increase internal production utilization. A sensitivity analysis was performed, to investigate the robustness of the configurations. Collectively, the framework has proven to provide feasible results within a wide range of parameters. This framework could help the preliminary investigation phase when analyzing future battery energy storage system investments.
Identification of natural disaster impacted electricity load profiles with k means clustering algorithm
Authors: Jessen Simon Hedegård , Ma Zheng Grace , Wijaya Francisco Danang , Vasquez Juan C. , Guerrero Josep , Jørgensen Bo Nørregaard
Published: December 2022

Natural disasters threat the resilience of the electricity system. However, little literature has investigated the electricity system’s recovering process and progress after natural disasters’ hit which strongly influence the system operators’ planning and quality of the security of supply for the electricity customers. To fill the research gap, this paper applies an unsupervised machine learning method, the k means clustering algorithm, to investigate the normal/abnormal electricity load profiles, identify natural disaster- and electrical fault-impacted electricity load profiles with a case study of the Lombok electricity system, Indonesia, and ½-hourly electricity load data from 2015 until 2021. The results show that electricity consumption in Lombok has increased over the years, which match the installed production capacity of Lombok. The results prove that the disturbance-induced electricity load patterns and especially natural disaster-impacted load profiles can be identified by the k means clustering algorithm. Especially, the pre-, during, and post-natural disaster impacted load patterns can be portrayed. Furthermore, the investigation results regarding the impacts of natural disasters and electrical faults on the performance of the electricity system, show that the natural disaster-induced load reductions and electrical fault-induced load reductions differ from the short and long-term perspectives. Moreover, the results can facilitate the electricity system operators to better understand the load patterns, predict ND strikes’ impact on the electricity system and conduct better long-term energy management strategies.
Progress and perspective on cotton breeding in Pakistan
Authors: Shahzad Kashif , Mubeen Iqra , Zhang Meng , Zhang Xuexian , Wu Jianyong , Xing Chaozhu
Published: December 2022

Cotton is the prime natural fiber with economic significance globally. Cotton farming and breeding have a long history in Pakistan. The development of high yielding upland cotton (Gossypium hirsutum) varieties gradually replaced the cultivation of diploid Gossypium species. Climate change along with emergence of new epidemic diseases caused yield loss in recent years. The biotic stress considerably reduced the performance and yield potential of cotton. Suitable breeding strategies are essential to generate useful genetic variations and to identify desired traits. Conventional breeding has remarkably increased cotton yield and fiber quality, which has cultivated the NIAB-78, S-12, MNH‐786, and FH‐Lalazar like cultivars. However, this phenotypic selection based breeding method has low efficiency to produce stress resilient cotton. The efficiency of traditional breeding has significantly improved by the marker assisted selection technology. Breakthroughs in molecular genetics, bioinformatics analysis, genetic engineering, and genome sequencing have opened new technique routes for cotton breeding. In addition, genetic improvement through quantitative trait loci, transcriptome, and CRISPR/Cas9 mediated genomic editing can provide suitable platform to improve the resistance to stresses induced by bollworms, cotton leaf curl virus, heat, drought, and salt. The approval of transgenic lines harboring triple gene Cry1Ac + Cry2A + GTG are critical for cotton crop. This review has critically discussed the progress and limitations of cotton breeding in Pakistan, and reviewed the utilization of novel genetic variations and selection tools for sustainable cotton production.
Influence of lauric acid on the relaxation of corpus cavernosum in streptozotocin-induced diabetic male Wistar rats
Authors: Olubiyi Makinde Vincent , Kawu Mohammed Umaru , Magaji Mohammed Garba , Salahdeen Hussein Mofomosara , Magaji Rabiu Abdulssalam
Published: December 2022

Background

Diabetes mellitus is a global health challenge and has been recognised as a risk factor for erectile dysfunction. Dissatisfaction with standard medications has been reported by some patients, hence therapeutic plants are being considered as a viable alternative therapy, with their active components being investigated to create a standard regimen. Lauric acid is the most abundant constituent of coconut oil and is proposed to be responsible for its therapeutic properties. The corpus cavernosum plays an important role in erectile function with its relaxation favouring erection. This study thus sought to investigate the possible relaxant action and mechanism of lauric acid on the corpus cavernosum of diabetic male Wistar rats. Diabetes was induced by intraperitoneal injection of streptozotocin after which graded doses of lauric acid were administered orally to three groups of diabetic rats, once daily for 4 weeks. At the end of the experiment, the corpus cavernosal tissues of the rat penis were extracted. Following phenylephrine or potassium chloride (KCl)—induced contraction, relaxation response to acetylcholine and sodium nitroprusside was used to evaluate endothelium-dependent and nitric oxide-mediated relaxation, respectively.

Results

Relaxation response to acetylcholine, following pre-contraction with phenylephrine, was significantly decreased in the cavernosal tissues of diabetic untreated rats and was not significantly improved in lauric acid treated diabetic groups. Relaxation response to acetylcholine, following pre-contraction with KCl, was significantly decreased in the diabetic untreated group but was significantly improved in lauric acid treated diabetic groups at the lowest dose. Decreased relaxation response to sodium nitroprusside, following pre-contraction with phenylephrine in tissues of diabetic untreated rats, was significantly improved in lauric acid-treated diabetic groups at lower doses. Decreased relaxation response to sodium nitroprusside, following pre-contraction with KCl, was significantly improved in lauric acid-treated diabetic groups at all doses.

Conclusion

Lauric acid improved relaxation of corpus cavernosum muscle in diabetic male rats by enhancing nitric oxide-mediated relaxing action of sodium nitroprusside and possibly inhibiting KCl-induced contraction.
The relationship between vascular endothelial growth factor-A serum level and the severity of diabetic peripheral neuropathy
Authors: Badrah Mai Hesham , Abdelaaty Talaat Abdelfattah , Imbaby Salma Alaa Eldin , Abdel-Fattah Yousra Hisham , Silim Walid Mohamed , El Feky Amr Yosry
Published: December 2022

Background and aims

Diabetic peripheral neuropathy (DPN) is a common microvascular complication in type 2 diabetes mellitus (T2DM). The nerve fibers injury is caused by the interaction between metabolic and vascular factors. Vascular endothelial growth factor (VEGF) is an essential growth factor for vascular endothelial cells. We aimed to investigate the relation between VEGF-A serum level and the degree of DPN.

Results

This cross-sectional study was conducted on 81 patients with T2DM. Based on the combined clinical and electrophysiological assessment, 67 patients (82.7%) were diagnosed with peripheral neuropathy of which 32 patients (39.5%) had subclinical neuropathy, whereas 35 patients (43.2%) were confirmed cases of DPN. Patients with DPN had longer duration of DM and higher values of glycosylated hemoglobin (HbA1c). Although the mean serum VEGF-A level in diabetic patients without neuropathy was higher than that in diabetic patients with DPN, this difference did not reach statistical significance (P = 0.07). However, patients with subclinical DPN had significantly higher serum VEGF-A level compared to patients with confirmed DPN (P < 0.001).

Conclusion

DPN was found to be a common finding in the studied sample of T2DM patients. Longer duration of DM and poor glycemic control may be risk factors for development of severe DPN. Low VEGF-A serum levels may lead to more severe DPN in patients with T2DM.
Intellectually disabled patients’ intensive care admission characteristics, weaning from mechanical ventilation, and sedative drug use: a single-center retrospective case-control study
Authors: Bineth Noa , Barel Nevo , Bdolah-Abram Tali , Levin Philip , Einav Sharon
Published: December 2022

Background

Intellectually disabled (ID) patients present unique therapeutic challenges. We aimed to describe the characteristics of ID patients admitted to a general intensive care unit (ICU).

Results

We conducted a retrospective cohort study comparing critically ill adult ID patients to matched patients without ID (1:2 ratio) in a single ICU (2010–2020). The main outcome measure was mortality. Secondary outcomes included complications during admission and characteristics of weaning from mechanical ventilation. The study and control groups were randomly selected based on similar age and sex. ID patients nonetheless had an average APACHE score of 18.5 ± 8.7 vs. 13.4 ± 8.5 in controls (p < 0.001). ID patients had more hematological (p = 0.04), endocrinological (p < 0.001) and neurological (p = 0.004) comorbidities and used more psychiatric medication before admission. No difference was found in mortality rates. Differences were found as there were more secondary complications, such as pulmonary and sepsis (p < 0.03), frequent requirement of vasopressors (p = 0.001), significantly higher intubation rates with more weaning attempts, tracheostomies and longer ICU and hospital admissions (p < 0.019).

Conclusions

Critically ill adult ID may have more comorbidities and be sicker at the time of admission compared to their age- and sex-matched counterparts. They require more supportive treatment and their weaning from mechanical ventilation may be more challenging.
Images of depression in Charles Baudelaire: clinical understanding in the context of poetry and social history
Authors: Stanghellini Giovanni , Ikkos George
Published: December 2022

There is increasing recognition of the importance of the humanities and arts in medical and psychiatric training. We explore the poetry of Charles Baudelaire (1821–1867) and its evocations of depression through themes of mood, time and self-consciousness and discuss their relation to images of ‘spleen’, the ‘snuffling clock’ and the ‘sinister mirror’. Following the literary critical commentaries of Walter Benjamin (1892–1940) and Jean Starobinski (1920–2019) we identify some of their roots in the poet's experience of the rapid and alienating urbanisation of 19th-century Paris. Appreciation of the rich vocabulary of poetry and the images it generates adds depth to clinical practice by painting vivid pictures of subjective experience, including subjective experience of the ‘social’ as part of the biopsychosocial constellation.
Age and Cohort Trends in Formal Volunteering and Informal Helping in Later Life: Evidence From the Health and Retirement Study
Authors: Han Sae Hwang , Shih Yao-Chi , Burr Jeffrey A. , Peng Changmin
Published: December 2022

Formal volunteering holds great importance for the recipients of volunteer services, individuals who volunteer, and the wider society. However, how much recent birth cohorts volunteer in middle and late adulthood compared with earlier birth cohorts is not well understood. Even less well-known are the age and cohort trends in informal helping provided to friends and neighbors in later adulthood. Using longitudinal data from the Health and Retirement Study, we estimated age and cohort trends in formal volunteering and informal helping from 1998 to 2018 for a wide range of birth cohorts born between 1909 and 1958. We used multivariate, multilevel models based on Bayesian generalized modeling methods to estimate the probabilities of volunteering and informal helping simultaneously in a single model. Despite having advantages in human and health capital, recent birth cohorts showed volunteering levels in late adulthood that are similar to those of their predecessors. Moreover, more recent birth cohorts were consistently less engaged in informal helping than earlier birth cohorts throughout the observation period. More research is needed to illuminate the sociocultural drivers of changes in helping behaviors and overall prosocial and civic engagement.