bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term Simulated Nitrogen Deposition Has Moderate Impacts on Soil Microbial Communities across Three Bioclimatic Domains of the Eastern Canadian Forest
Authors: Renaudin Marie , Khlifa Rim , Legault Simon , Kembel Steven W. , Kneeshaw Daniel , Moore Jean-David , Houle Daniel
Published: May 2023

The soil microbiome plays major roles in the below-ground processes and productivity of forest ecosystems. Atmospheric nitrogen (N) deposition is predicted to increase globally and might create disturbances in soil microbial communities, essentially by modifying soil chemistry. However, the impacts of higher N deposition on the soil microbiome in N-limited northern forests are still unclear. For 16 years, we simulated N deposition by adding ammonium nitrate at rates of 3 and 10 times the ambient N deposition directly into soils located in three bioclimatic domains of the eastern Canadian forest (i.e., sugar maple–yellow birch, balsam fir–white birch, and black spruce–feather moss). We identified changes in the microbial communities by isolating the DNA of the L, F, and H soil horizons, as well as by sequencing amplicons of the bacterial 16S rRNA gene and the fungal ITS region. We found that long-term increased N deposition had no effect on soil microbial diversity, but had moderate impacts on the composition of the bacterial and fungal communities. The most noticeable change was the increase in ectomycorrhizal fungi ASV abundance, potentially due to increased tree root growth on fertilized plots. Our work suggests that, in N-limited northern forests, extra N is rapidly mobilized by vegetation, thus minimizing impacts on the soil microbiome.
Differences in Recreational Fishers’ Motivations for Utilising Two Estuarine Fisheries
Authors: Tweedley James R. , Obregón Clara , Beukes Sarah J. , Loneragan Neil R. , Hughes Michael
Published: May 2023

Effective fisheries management requires an understanding of human dimensions. This study elicited the salient motivations for recreational blue swimmer crab and black bream fishing in Western Australia and whether these views differed depending on the fishing location and/or the characteristics of the fisher. Crab fishers were strongly consumption-orientated and aimed to “catch big crabs” and “catch enough crabs to eat”. Furthermore, 91% consumed their catch, with only 2% practicing catch-and-release fishing. In contrast, 81% of black bream fishers did so for the sport/challenge, with the strongest motivation being to catch a bream considerably above legal size and with food only selected by 15% of respondents; most fishers released caught fish. The marked differences between the fisheries for the two species, which co-occur in the same estuaries, are likely driven by the accessible nature of the crab fishery, ease of catching crabs, the low cost of fishing equipment, and their taste. Fishing for black bream, however, requires more expensive equipment, patience, and a greater skill level. Fishers considered crabbing to be as important as other fishing and outdoor activities, whereas bream fishers considered bream fishing considerably more important, reflecting the trophy nature of this fishery.
pH Modulated Formation of Complexes with Various Stoichiometry between Polymer Network and Fe(III) in Thermosensitive Gels Modified with Gallic Acid
Authors: Kaniewska Klaudia , Kościelniak Patrycja , Karbarz Marcin
Published: May 2023

Thermoresponsive gels based on N-isopropylacrylamide functionalized with amino groups were modified with gallic acid, with gallate (3,4,5-trihydroxybenzoic) groups being introduced into the polymer network. We investigated how the properties of these gels were affected at varying pH, by the formation of complexes between the polymer network of the gels and Fe3+ ions (which form stable complexes with gallic acid, exhibiting 1:1, 1:2, or 1:3 stoichiometry, depending on pH). The formation of complexes with varying stoichiometry within the gel was confirmed using UV-Vis spectroscopy, and the influence of such complexes on swelling behavior and volume phase transition temperature were investigated. In the appropriate temperature range, complex stoichiometry was found to strongly affect the swelling state. Changes in the pore structure and mechanical properties of the gel caused by the formation of complexes with varying stoichiometry were investigated using scanning electron microscopy and rheological measurements, respectively. The volume changes exhibited by p(NIPA-5%APMA)-Gal-Fe gel were found to be greatest at close to human body temperature (~38 °C). Modification of thermoresponsive pNIPA gel with gallic acid opens new opportunities for the development of pH- and thermosensitive gel materials.
Pregnancy in Patients with Moderate and Highly Complex Congenital Heart Disease
Authors: Panebianco Mario , Perrone Marco Alfonso , Gagliardi Maria Giulia , Galletti Lorenzo , Bassareo Pier Paolo
Published: May 2023

Although not completely devoid of risk, pregnancy can be managed in virtually all patients affected by even the most complex forms of congenital heart disease. It is not however advisable in patients with any form of pulmonary arterial hypertension. Pregnancy is even manageable in patients with univentricular heart converted to Fontan circulation. A personalised risk stratification should be performed, and patients affected by advanced NYHA functional class appropriately warned of the potential risks. In this setting, metabolomics might represent a novel tool for use in conducting personalised risk stratification. All pregnancies, particularly those at higher risk, should be managed in a tertiary care centre capable of providing the necessary assistance to both the mother and infant. With a few rare exceptions, vaginal delivery is to be preferred over caesarean section due to the lower degree of maternal and foetal complications. The desire for motherhood, at times extreme in women with congenital heart disease, may often be accomplished, thus providing a ray of hope in the lives of these patients.
Simultaneous Trunk and Canopy Shaking Improves Table Olive Harvester Efficiency versus Trunk Shaking Alone
Authors: Homayouni Taymaz , Maharlooei Mohammadmehdi , Toudeshki Arash , Ferguson Louise , Ehsani Reza
Published: May 2023

Production of California table olives has declined significantly in recent years due to hand harvesting costs, often over 60% of gross return. Mechanical harvesting could sharply decrease harvest costs, increasing economic viability. Mechanical harvester efficiency is a combination of the percentage of the total fruit on a tree removed by a harvester, and the time required to do so. A comparison between an experimental canopy contact shaker and a commercial trunk shaker demonstrated low harvest efficiencies and no significant differences in harvester efficiency between the two, averaging no more than 8%. However, simultaneously combining both shaking methods increased fruit removal to an economically feasible 75% and produced better fruit quality. Combining both shaking methods increased the price per ton by 63% versus trunk shaking and 35% versus canopy shaking. These results suggest a mechanical olive harvester that simultaneously combines trunk and canopy shaking is more efficient than either shaking method alone, and, has potential for economically feasible mechanical table olive harvesting.
‘Be the Match’. Predictors of Decisions Concerning Registration as a Potential Bone Marrow Donor—A Psycho-Socio-Demographic Study
Authors: Bogucki Jacek , Tuszyńska-Bogucka Wioletta
Published: May 2023

(1) Background: The study was aimed at a better understanding of the factors determining making a decision to become a potential bone marrow donor, in a Polish research sample; (2) Methods: The data was collected using a self-report questionnaire among persons who voluntarily participated in the study concerning donation, conducted on a sample of the Polish population via Internet. The study included 533 respondents (345 females and 188 males), aged 18–49. Relationships between the decision about registration as potential bone marrow donor and psycho-socio-demographic factors were estimated using the machine learning methods (binary logistic regression and classification & regression tree); (3) Results. The applied methods coherently emphasized the crucial role of personal experiences in making the decision about willingness for potential donation, f.e. familiarity with the potential donor. They also indicated religious issues and negative health state assessment as main decision-making destimulators; (4) Conclusions. The results of the study may contribute to an increase in the effectiveness of recruitment actions by more precise personalization of popularizing-recruitment actions addressed to the potential donors. It was found that selected machine learning methods are interesting set of analyses, increasing the prognostic accuracy and quality of the proposed model.
Ovarian Cancer Biomarkers in the COVID-19 Era
Authors: Farina Antonella , Colaiacovo Flavia , Gianfrate Mariacarmela , Pucci Beatrice , Angeloni Antonio , Anastasi Emanuela
Published: May 2023

Ovarian Cancer (OC) diagnosis is entrusted to CA125 and HE4. Since the latter has been found increased in COVID-19 patients, in this study, we aimed to evaluate the influence of SARS-CoV-2 infection on OC biomarkers. HE4 values above the cut-off were observed in 65% of OC patients and in 48% of SARS-CoV-2-positive patients (not oncologic patients), whereas CA125 values above the cut-off were observed in 71% of OC patients and in 11% of SARS-CoV-2 patients. Hence, by dividing the HE4 levels into quartiles, we can state that altered levels of HE4 in COVID-19 patients were mostly detectable in quartile I (151–300 pmol/L), while altered levels in OC patients were mostly clustered in quartile III (>600, pmol/L). In light of these observations, in order to better discriminate women with ovarian cancer versus those with COVID-19, we established a possible HE4 cut-off of 328 pmol/L by means of a ROC curve. These results demonstrate that the reliability of HE4 as a biomarker in ovarian cancer remains unchanged, despite COVID-19 interference; moreover, it is important for a proper diagnosis that whether the patient has a recent history of SARS-CoV-2 infection is determined.
Social Correlates of HIV-Risky Behaviours among African Canadian Adolescents Living in British Columbia, Canada: A Secondary Data Analysis
Authors: Ojukwu Emmanuela Nneamaka , Okoye Helen Uche , Saewyc Elizabeth
Published: June 2023

Studies have linked HIV-risky behaviours among young people to several socio-contextual factors. However, the social factors that might increase African Canadian adolescents’ exposure to HIV-risky behaviours, including unprotected sex and forced or multiple-sexual partnerships, have received little or no attention in the literature. Using data from the British Columbia Adolescent Health Surveys (2003–2018) and guided by intersectionality and socio-ecological frameworks, we examined the social determinants of HIV-risky behaviours (HRB) among African Canadian adolescents in British Columbia. We observed a general decline in HRB from 2008 to 2018. However, more than half (54.5%) of the 1042 who were sexually experienced in 2018 reported having 2 or more sexual partners, and nearly half reported condom-less sex. Our results demonstrate an important need to evaluate the impacts of several social factors on health outcomes for a unique, marginalized population.
Plakophilin-3 Binds the Membrane and Filamentous Actin without Bundling F-Actin
Authors: Gupta Jyoti , Rangarajan Erumbi S. , Troyanovsky Regina B. , Indra Indrajyoti , Troyanovsky Sergey M. , Izard Tina
Published: May 2023

Plakophilin-3 is a ubiquitously expressed protein found widely in epithelial cells and is a critical component of desmosomes. The plakophilin-3 carboxy-terminal domain harbors nine armadillo repeat motifs with largely unknown functions. Here, we report the 5 Å cryogenic electron microscopy (cryoEM) structure of the armadillo repeat motif domain of plakophilin-3, one of the smaller cryoEM structures reported to date. We find that this domain is a monomer or homodimer in solution. In addition, using an in vitro actin co-sedimentation assay, we show that the armadillo repeat domain of plakophilin-3 directly interacts with F-actin. This feature, through direct interactions with actin filaments, could be responsible for the observed association of extra-desmosomal plakophilin-3 with the actin cytoskeleton directly attached to the adherens junctions in A431 epithelial cells. Further, we demonstrate, through lipid binding analyses, that plakophilin-3 can effectively be recruited to the plasma membrane through phosphatidylinositol-4,5-bisphosphate-mediated interactions. Collectively, we report on novel properties of plakophilin-3, which may be conserved throughout the plakophilin protein family and may be behind the roles of these proteins in cell–cell adhesion.
Correction: Song et al. Protective Effect of Resveratrol in an Experimental Model of Salicylate-Induced Tinnitus. Int. J. Mol. Sci. 2022, 23, 14183
Authors: Song Anji , Cho Gwang-Won , Moon Changjong , Park Ilyong , Jang Chul Ho
Published: May 2023

In the original publication [...]
Transcriptome-Wide Analysis of Low-Concentration Exposure to Bisphenol A, S, and F in Prostate Cancer Cells
Authors: Cortés-Ramírez Sergio A. , Salazar Ana M. , Sordo Monserrat , Ostrosky-Wegman Patricia , Morales-Pacheco Miguel , Cruz-Burgos Marian , Losada-García Alberto , Rodríguez-Martínez Griselda , González-Ramírez Imelda , Vazquez-Santillan Karla , González-Covarrubias Vanessa , Maldonado-Lagunas Vilma , Rodríguez-Dorantes Mauricio
Published: May 2023

Bisphenol A (BPA) is a ubiquitous synthetic compound used as a monomer in the production of polycarbonate plastics and epoxy resins. Even at low doses, BPA has been associated with the molecular progression of diseases such as obesity, metabolic syndrome, and hormone-regulated cancers due to its activity as an endocrine-disrupting chemical (EDC). Consequently, the use of BPA has been regulated worldwide by different health agencies. BPA structural analogs such as bisphenol S and bisphenol F (BPS and BPF) have emerged as industrial alternatives, but their biological activity in the molecular progression of cancer remains unclear. Prostate cancer (PCa) is a hormone-dependent cancer, and the role of BPA structural analogs in PCa progression is still undescribed. In this work, we use an in vitro model to characterize the transcriptomic effect of low-concentration exposure to bisphenol A, S, or F in the two main stages of the disease: androgen dependency (LNCaP) and resistance (PC-3). Our findings demonstrated that the low concentration exposure to each bisphenol induced a differential effect over PCa cell lines, which marks the relevance of studying the effect of EDC compounds through all the stages of the disease.
Cross-Dressing of Multiple Myeloma Cells Mediated by Extracellular Vesicles Conveying MIC and ULBP Ligands Promotes NK Cell Killing
Authors: Vulpis Elisabetta , Loconte Luisa , Cassone Chiara , Antonangeli Fabrizio , Caracciolo Giulio , Masuelli Laura , Fazio Francesca , Petrucci Maria Teresa , Fionda Cinzia , Soriani Alessandra , Cerboni Cristina , Cippitelli Marco , Santoni Angela , Zingoni Alessandra
Published: May 2023

Natural Killer (NK) cells are innate cytotoxic lymphoid cells that play a crucial role in cancer immunosurveillance. NKG2D is an activating receptor that binds to MIC and ULBP molecules typically induced on damaged, transformed, or infected cells. The secretion of NKG2D ligands (NKG2DLs) through protease-mediated cleavage or in an extracellular vesicle (EV) is a mode to control their cell surface expression and a mechanism used by cancer cells to evade NKG2D-mediated immunosurveillance. EVs are emerging as important players in mediating cell-to-cell communication due to their ability to transfer biological material to acceptor cells. Herein, we investigated the spreading of NKG2DLs of both MIC and ULBP molecules through the EV-mediated cross-dressing on multiple myeloma (MM) cells. We focused our attention on two MICA allelic variants, namely MICA*008 and MICA*019, representing the prototype of short and long MICA alleles, respectively, and on ULBP-1, ULBP-2, and ULBP-3. Our findings demonstrate that both ULBP and MICA ligands can be acquired from tumor cells through EVs enhancing NK cell recognition and killing. Moreover, besides MICA, EVs expressing ULBP-1 but not ULBP-2 and 3 were detected in bone marrow aspirates derived from a cohort of MM patients. Our findings shed light on the role of EV-associated MICA allelic variants and ULBP molecules in the modulation of NKG2D-mediated NK cell immunosurveillance in the tumor microenvironment. Moreover, the EV-mediated transfer of NKG2DLs could suggest novel therapeutic approaches based on the usage of engineered nanoparticles aimed at increasing cancer cell immunogenicity.
Visualization of Cellular Membranes in 2D and 3D Conditions Using a New Fluorescent Dithienothiophene S,S-Dioxide Derivative
Authors: Rzewnicka Aneta , Krysiak Jerzy , Pawłowska Róża , Żurawiński Remigiusz
Published: June 2023

Cellular membranes play a key role in cell communication with the extracellular environment and neighboring cells. Any changes, including their composition, packing, physicochemical properties and formation of membrane protrusions may affect cells feature. Despite its great importance, tracking membrane changes in living cells is still a challenge. For investigation of processes related to tissue regeneration and cancer metastasis, such as the induction of epithelial-mesenchymal transition, increased cell motility, and blebbing, the possibility to conduct prolonged observation of membrane changes is beneficial, albeit difficult. A particular challenge is conducting this type of research under detachment conditions. In the current manuscript, a new dithienothiophene S,S-dioxide (DTTDO) derivative is presented as an effective dye for staining the membranes of living cells. The synthetic procedures, physicochemical properties, and biological activity of the new compound are presented herein. In addition to the labeling of the membranes in a monolayer culture, its usefulness for visualization of membranes under detachment conditions is also demonstrated. Obtained data have proven that a new DTTDO derivative may be used to stain membranes in various types of experimental procedures, from traditional 2D cell cultures to unanchored conditions. Moreover, due to the specific optical properties, the background signal is reduced and, thus, observation may be performed without washing.
Screening and Analysis of Possible Drugs Binding to PDGFRα: A Molecular Modeling Study
Authors: Mozzicafreddo Matteo , Benfaremo Devis , Paolini Chiara , Agarbati Silvia , Svegliati Baroni Silvia , Moroncini Gianluca
Published: June 2023

The platelet-derived growth factor receptor (PDGFR) is a membrane tyrosine kinase receptor involved in several metabolic pathways, not only physiological but also pathological, as in tumor progression, immune-mediated diseases, and viral diseases. Considering this macromolecule as a druggable target for modulation/inhibition of these conditions, the aim of this work was to find new ligands or new information to design novel effective drugs. We performed an initial interaction screening with the human intracellular PDGFRα of about 7200 drugs and natural compounds contained in 5 independent databases/libraries implemented in the MTiOpenScreen web server. After the selection of 27 compounds, a structural analysis of the obtained complexes was performed. Three-dimensional quantitative structure–activity relationship (3D-QSAR) and absorption, distribution, metabolism, excretion, and toxicity (ADMET) analyses were also performed to understand the physicochemical properties of identified compounds to increase affinity and selectivity for PDGFRα. Among these 27 compounds, the drugs Bafetinib, Radotinib, Flumatinib, and Imatinib showed higher affinity for this tyrosine kinase receptor, lying in the nanomolar order, while the natural products included in this group, such as curcumin, luteolin, and epigallocatechin gallate (EGCG), showed sub-micromolar affinities. Although experimental studies are mandatory to fully understand the mechanisms behind PDGFRα inhibitors, the structural information obtained through this study could provide useful insight into the future development of more effective and targeted treatments for PDGFRα-related diseases, such as cancer and fibrosis.
Toll-like Receptor Response to Human Immunodeficiency Virus Type 1 or Co-Infection with Hepatitis B or C Virus: An Overview
Authors: Kayesh Mohammad Enamul Hoque , Kohara Michinori , Tsukiyama-Kohara Kyoko
Published: June 2023

Toll-like receptors (TLRs) are evolutionarily conserved pattern recognition receptors that play important roles in the early detection of pathogen-associated molecular patterns and shaping innate and adaptive immune responses, which may influence the consequences of infection. Similarly to other viral infections, human immunodeficiency virus type 1 (HIV-1) also modulates the host TLR response; therefore, a proper understanding of the response induced by human HIV-1 or co-infection with hepatitis B virus (HBV) or hepatitis C virus (HCV), due to the common mode of transmission of these viruses, is essential for understanding HIV-1 pathogenesis during mono- or co-infection with HBV or HCV, as well as for HIV-1 cure strategies. In this review, we discuss the host TLR response during HIV-1 infection and the innate immune evasion mechanisms adopted by HIV-1 for infection establishment. We also examine changes in the host TLR response during HIV-1 co-infection with HBV or HCV; however, this type of study is extremely scarce. Moreover, we discuss studies investigating TLR agonists as latency-reverting agents and immune stimulators towards new strategies for curing HIV. This understanding will help develop a new strategy for curing HIV-1 mono-infection or co-infection with HBV or HCV.
Pre-Treatment of Rice Plants with ABA Makes Them More Tolerant to Multiple Abiotic Stress
Authors: Habibpourmehraban Fatemeh , Wu Yunqi , Masoomi-Aladizgeh Farhad , Amirkhani Ardeshir , Atwell Brian J. , Haynes Paul A.
Published: June 2023

Multiple abiotic stress is known as a type of environmental unfavourable condition maximizing the yield and growth gap of crops compared with the optimal condition in both natural and cultivated environments. Rice is the world’s most important staple food, and its production is limited the most by environmental unfavourable conditions. In this study, we investigated the pre-treatment of abscisic acid (ABA) on the tolerance of the IAC1131 rice genotype to multiple abiotic stress after a 4-day exposure to combined drought, salt and extreme temperature treatments. A total of 3285 proteins were identified and quantified across the four treatment groups, consisting of control and stressed plants with and without pre-treatment with ABA, with 1633 of those proteins found to be differentially abundant between groups. Compared with the control condition, pre-treatment with the ABA hormone significantly mitigated the leaf damage against combined abiotic stress at the proteome level. Furthermore, the application of exogenous ABA did not affect the proteome profile of the control plants remarkably, while the results were different in stress-exposed plants by a greater number of proteins changed in abundance, especially those which were increased. Taken together, these results suggest that exogenous ABA has a potential priming effect for enhancing the rice seedlings’ tolerance against combined abiotic stress, mainly by affecting stress-responsive mechanisms dependent on ABA signalling pathways in plants.
Iron and Ferroptosis More than a Suspect: Beyond the Most Common Mechanisms of Neurodegeneration for New Therapeutic Approaches to Cognitive Decline and Dementia
Authors: Cerasuolo Michele , Di Meo Irene , Auriemma Maria Chiara , Trojsi Francesca , Maiorino Maria Ida , Cirillo Mario , Esposito Fabrizio , Polito Rita , Colangelo Anna Maria , Paolisso Giuseppe , Papa Michele , Rizzo Maria Rosaria
Published: June 2023

Neurodegeneration is a multifactorial process that involves multiple mechanisms. Examples of neurodegenerative diseases are Parkinson’s disease, multiple sclerosis, Alzheimer’s disease, prion diseases such as Creutzfeldt–Jakob’s disease, and amyotrophic lateral sclerosis. These are progressive and irreversible pathologies, characterized by neuron vulnerability, loss of structure or function of neurons, and even neuron demise in the brain, leading to clinical, functional, and cognitive dysfunction and movement disorders. However, iron overload can cause neurodegeneration. Dysregulation of iron metabolism associated with cellular damage and oxidative stress is reported as a common event in several neurodegenerative diseases. Uncontrolled oxidation of membrane fatty acids triggers a programmed cell death involving iron, ROS, and ferroptosis, promoting cell death. In Alzheimer’s disease, the iron content in the brain is significantly increased in vulnerable regions, resulting in a lack of antioxidant defenses and mitochondrial alterations. Iron interacts with glucose metabolism reciprocally. Overall, iron metabolism and accumulation and ferroptosis play a significant role, particularly in the context of diabetes-induced cognitive decline. Iron chelators improve cognitive performance, meaning that brain iron metabolism control reduces neuronal ferroptosis, promising a novel therapeutic approach to cognitive impairment.
Lipid Membrane Remodeling by the Micellar Aggregation of Long-Chain Unsaturated Fatty Acids for Sustainable Antimicrobial Strategies
Authors: Shin Sungmin , Tae Hyunhyuk , Park Soohyun , Cho Nam-Joon
Published: June 2023

Antimicrobial fatty acids derived from natural sources and renewable feedstocks are promising surface-active substances with a wide range of applications. Their ability to target bacterial membrane in multiple mechanisms offers a promising antimicrobial approach for combating bacterial infections and preventing the development of drug-resistant strains, and it provides a sustainable strategy that aligns with growing environmental awareness compared to their synthetic counterparts. However, the interaction and destabilization of bacterial cell membranes by these amphiphilic compounds are not yet fully understood. Here, we investigated the concentration-dependent and time-dependent membrane interaction between long-chain unsaturated fatty acids—linolenic acid (LNA, C18:3), linoleic (LLA, C18:2), and oleic acid (OA, C18:1)—and the supported lipid bilayers (SLBs) using quartz crystal microbalance-dissipation (QCM-D) and fluorescence microscopy. We first determined the critical micelle concentration (CMC) of each compound using a fluorescence spectrophotometer and monitored the membrane interaction in real time following fatty acid treatment, whereby all micellar fatty acids elicited membrane-active behavior primarily above their respective CMC values. Specifically, LNA and LLA, which have higher degrees of unsaturation and CMC values of 160 µM and 60 µM, respectively, caused significant changes in the membrane with net |Δf| shifts of 23.2 ± 0.8 Hz and 21.4 ± 0.6 Hz and ΔD shifts of 5.2 ± 0.5 × 10−6 and 7.4 ± 0.5 × 10−6. On the other hand, OA, with the lowest unsaturation degree and CMC value of 20 µM, produced relatively less membrane change with a net |Δf| shift of 14.6 ± 2.2 Hz and ΔD shift of 8.8 ± 0.2 × 10−6. Both LNA and LLA required higher concentrations than OA to initiate membrane remodeling as their CMC values increased with the degree of unsaturation. Upon incubating with fluorescence-labeled model membranes, the fatty acids induced tubular morphological changes at concentrations above CMC. Taken together, our findings highlight the critical role of self-aggregation properties and the degree of unsaturated bonds in unsaturated long-chain fatty acids upon modulating membrane destabilization, suggesting potential applications in developing sustainable and effective antimicrobial strategies.
Pro- and Anti-Inflammatory Prostaglandins and Cytokines in Humans: A Mini Review
Authors: Wautier Jean-Luc , Wautier Marie-Paule
Published: June 2023

Inflammation has been described for two millennia, but cellular aspects and the paradigm involving different mediators have been identified in the recent century. Two main groups of molecules, the prostaglandins (PG) and the cytokines, have been discovered and play a major role in inflammatory processes. The activation of prostaglandins PGE2, PGD2 and PGI2 results in prominent symptoms during cardiovascular and rheumatoid diseases. The balance between pro- and anti-inflammatory compounds is nowadays a challenge for more targeted therapeutic approaches. The first cytokine was described more than a century ago and is now a part of different families of cytokines (38 interleukins), including the IL-1 and IL-6 families and TNF and TGFβ families. Cytokines can perform a dual role, being growth promotors or inhibitors and having pro- and anti-inflammatory properties. The complex interactions between cytokines, vascular cells and immune cells are responsible for dramatic conditions and lead to the concept of cytokine storm observed during sepsis, multi-organ failure and, recently, in some cases of COVID-19 infection. Cytokines such as interferon and hematopoietic growth factor have been used as therapy. Alternatively, the inhibition of cytokine functions has been largely developed using anti-interleukin or anti-TNF monoclonal antibodies in the treatment of sepsis or chronic inflammation.
TREX1 531C/T Polymorphism and Autoantibodies Associated with the Immune Status of HIV-1-Infected Individuals
Authors: Queiroz Maria Alice Freitas , Moura Tuane Carolina Ferreira , Bichara Carlos David Araújo , Lima Lorena Leticia Peixoto de , Oliveira Allysson Quintino Tenório de , Souza Ranilda Gama de , Gomes Samara Tatielle Monteiro , Amoras Ednelza da Silva Graça , Vallinoto Antonio Carlos Rosário
Published: June 2023

Autoimmune diseases can develop during HIV-1 infection, mainly related to the individual’s immune competence. The study investigated the association of the TREX1 531C/T polymorphism and antinuclear antibodies (ANA) in HIV-1 infection and the time of antiretroviral therapy (ART) used. Cross-sectional and longitudinal assessments were carried out in 150 individuals, divided into three groups: ART-naïve, 5 years and 10 years on ART; ART-naïve individuals were evaluated for 2 years after initiation of treatment. The individuals’ blood samples were submitted to indirect immunofluorescence tests, real-time PCR and flow cytometry. The TREX1 531C/T polymorphism was associated with higher levels of TCD4+ lymphocytes and IFN-α in individuals with HIV-1. Individuals on ART had a higher frequency of ANA, higher levels of T CD4+ lymphocytes, a higher ratio of T CD4+/CD8+ lymphocytes and higher levels of IFN-α than therapy-naïve individuals (p < 0.05). The TREX1 531C/T polymorphism was associated with better maintenance of the immune status of individuals with HIV-1 and ANA with immune restoration in individuals on ART, indicating the need to identify individuals at risk of developing an autoimmune disease.
Advances in Sol-Gel-Based Superhydrophobic Coatings for Wood: A Review
Authors: Wang Yudong , Ge-Zhang Shangjie , Mu Pingxuan , Wang Xueqing , Li Shaoyi , Qiao Lingling , Mu Hongbo
Published: June 2023

As the focus of architecture, furniture, and other fields, wood has attracted extensive attention for its many advantages, such as environmental friendliness and excellent mechanical properties. Inspired by the wetting model of natural lotus leaves, researchers prepared superhydrophobic coatings with strong mechanical properties and good durability on the modified wood surface. The prepared superhydrophobic coating has achieved functions such as oil-water separation and self-cleaning. At present, some methods such as the sol-gel method, the etching method, graft copolymerization, and the layer-by-layer self-assembly method can be used to prepare superhydrophobic surfaces, which are widely used in biology, the textile industry, national defense, the military industry, and many other fields. However, most methods for preparing superhydrophobic coatings on wood surfaces are limited by reaction conditions and process control, with low coating preparation efficiency and insufficiently fine nanostructures. The sol-gel process is suitable for large-scale industrial production due to its simple preparation method, easy process control, and low cost. In this paper, the research progress on wood superhydrophobic coatings is summarized. Taking the sol-gel method with silicide as an example, the preparation methods of superhydrophobic coatings on wood surfaces under different acid-base catalysis processes are discussed in detail. The latest progress in the preparation of superhydrophobic coatings by the sol-gel method at home and abroad is reviewed, and the future development of superhydrophobic surfaces is prospected.
Type 2 Diabetes Mellitus, Non-Alcoholic Fatty Liver Disease, and Metabolic Repercussions: The Vicious Cycle and Its Interplay with Inflammation
Authors: Frankowski Rafał , Kobierecki Mateusz , Wittczak Andrzej , Różycka-Kosmalska Monika , Pietras Tadeusz , Sipowicz Kasper , Kosmalski Marcin
Published: June 2023

The prevalence of metabolic-related disorders, such as non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (DM2), has been increasing. Therefore, developing improved methods for the prevention, treatment, and detection of these two conditions is also necessary. In this study, our primary focus was on examining the role of chronic inflammation as a potential link in the pathogenesis of these diseases and their interconnections. A comprehensive search of the PubMed database using keywords such as “non-alcoholic fatty liver disease”, “type 2 diabetes mellitus”, “chronic inflammation”, “pathogenesis”, and “progression” yielded 177 relevant papers for our analysis. The findings of our study revealed intricate relationships between the pathogenesis of NAFLD and DM2, emphasizing the crucial role of inflammatory processes. These connections involve various molecular functions, including altered signaling pathways, patterns of gene methylation, the expression of related peptides, and up- and downregulation of several genes. Our study is a foundational platform for future research into the intricate relationship between NAFLD and DM2, allowing for a better understanding of the underlying mechanisms and the potential for introducing new treatment standards.
Rheumatologist’s Perspective on Non-Infectious Uveitis: Patterns from Tertiary Referral Rheumatologic Clinics in Italy
Authors: Triggianese Paola , Fatica Mauro , Caso Francesco , Costa Luisa , D’Antonio Arianna , Tasso Marco , Greco Elisabetta , Conigliaro Paola , Bergamini Alberto , Fabiani Claudia , Cantarini Luca , Chimenti Maria Sole
Published: June 2023

Non-infectious uveitis (NIU) can be an early or even the first extra-articular manifestation of systemic rheumatic diseases, or the first one; thus, rheumatologists are often involved in the diagnostic and therapeutic assessment of NIU. We evaluated 130 patients with a diagnosis of NIU who were admitted to two Italian rheumatologic clinics (Tor Vergata University Hospital in Rome, and Federico II University in Naples) from January 2018 to December 2021. Anterior uveitis (AU) occurred in 75.4% of patients, followed by posterior uveitis (PU, 21.5%); acute (54.6%) and recurrent (35.4%) NIU were more documented than chronic NIU (10%), and a bilateral involvement was observed in 38.7% of cases. Half of NIU cases were associated with spondyloarthritis (SpA); the remaining were affected by Behçet disease (BD)-related uveitis (13.9%) and idiopathic NIU (9.2%). HLA-B27+ patients (34.8%) had a higher prevalence of anterior and unilateral NIU (p = 0.005) with acute course (p = 0.04) than HLA-B27– patients. On the contrary, HLA-B51+ patients (19.6%) had mostly PU and bilateral NIU (p < 0.0001) and recurrent course (p = 0.04) than HLA-B51– patients. At the first rheumatologic referral, 117 patients (90%) received systemic treatments. Findings from this study demonstrate that rheumatologic referral has a pivotal role in the diagnostic work-up of NIU and may dramatically influence NIU-treatment strategies.
Cannabidiol for Oral Health: A New Promising Therapeutical Tool in Dentistry
Authors: Bellocchio Luigi , Patano Assunta , Inchingolo Alessio Danilo , Inchingolo Francesco , Dipalma Gianna , Isacco Ciro Gargiulo , de Ruvo Elisabetta , Rapone Biagio , Mancini Antonio , Lorusso Felice , Scarano Antonio , Malcangi Giuseppina , Inchingolo Angelo Michele
Published: June 2023

The medical use of cannabis has a very long history. Although many substances called cannabinoids are present in cannabis, Δ9tetrahydrocannabinol (Δ9-THC), cannabidiol (CBD) and cannabinol (CBN) are the three main cannabinoids that are most present and described. CBD itself is not responsible for the psychotropic effects of cannabis, since it does not produce the typical behavioral effects associated with the consumption of this drug. CBD has recently gained growing attention in modern society and seems to be increasingly explored in dentistry. Several subjective findings suggest some therapeutic effects of CBD that are strongly supported by research evidence. However, there is a plethora of data regarding CBD’s mechanism of action and therapeutic potential, which are in many cases contradictory. We will first provide an overview of the scientific evidence on the molecular mechanism of CBD’s action. Furthermore, we will map the recent developments regarding the possible oral benefits of CBD. In summary, we will highlight CBD’s promising biological features for its application in dentistry, despite exiting patents that suggest the current compositions for oral care as the main interest of the industry.
METRIC—Multi-Eye to Robot Indoor Calibration Dataset
Authors: Allegro Davide , Terreran Matteo , Ghidoni Stefano
Published: May 2023

Multi-camera systems are an effective solution for perceiving large areas or complex scenarios with many occlusions. In such a setup, an accurate camera network calibration is crucial in order to localize scene elements with respect to a single reference frame shared by all the viewpoints of the network. This is particularly important in applications such as object detection and people tracking. Multi-camera calibration is a critical requirement also in several robotics scenarios, particularly those involving a robotic workcell equipped with a manipulator surrounded by multiple sensors. Within this scenario, the robot-world hand-eye calibration is an additional crucial element for determining the exact position of each camera with respect to the robot, in order to provide information about the surrounding workspace directly to the manipulator. Despite the importance of the calibration process in the two scenarios outlined above, namely (i) a camera network, and (ii) a camera network with a robot, there is a lack of standard datasets available in the literature to evaluate and compare calibration methods. Moreover they are usually treated separately and tested on dedicated setups. In this paper, we propose a general standard dataset acquired in a robotic workcell where calibration methods can be evaluated in two use cases: camera network calibration and robot-world hand-eye calibration. The Multi-Eye To Robot Indoor Calibration (METRIC) dataset consists of over 10,000 synthetic and real images of ChAruCo and checkerboard patterns, each one rigidly attached to the robot end-effector, which was moved in front of four cameras surrounding the manipulator from different viewpoints during the image acquisition. The real images in the dataset includes several multi-view image sets captured by three different types of sensor networks: Microsoft Kinect V2, Intel RealSense Depth D455 and Intel RealSense Lidar L515, to evaluate their advantages and disadvantages for calibration. Furthermore, in order to accurately analyze the effect of camera-robot distance on calibration, we acquired a comprehensive synthetic dataset, with related ground truth, with three different camera network setups corresponding to three levels of calibration difficulty depending on the cell size. An additional contribution of this work is to provide a comprehensive evaluation of state-of-the-art calibration methods using our dataset, highlighting their strengths and weaknesses, in order to outline two benchmarks for the two aforementioned use cases.
Multiple Aspects of the Fight against the Red Palm Weevil in an Urban Area: Study Case, San Benedetto del Tronto (Central Italy)
Authors: Bracchetti Luca , Cocci Paolo , Palermo Francesco Alessandro
Published: May 2023

The fight against alien invasive insect pests of plants in the urban environment often affects varied sectors of the economy, landscape gardening, public health, and ecology. This paper focuses on the evolution of the red palm weevil in San Benedetto del Tronto, a coastal urban area in central Italy. We investigated the evolution of this insect pest of palm trees in the 2013–2020 period, considering both the effectiveness of the chemicals used and their potentially harmful effects. With a multidisciplinary approach, we carried out a spatio-temporal analysis of the extent and mode of pest spread over time using historical aerial photos, freely available remote sensing images, and field surveys integrated in a GIS environment. We also assessed the toxicity risk associated with the chemicals used to protect the palms from the red weevil. The fight against this weevil is now concentrated in specific areas such as parks, roads, villas, hotels, farmhouses, and nurseries. The preventive chemical treatments applied are very effective in preserving the palms, but they show a toxic potential for all organisms. We discuss current local management of this pest, focusing on several aspects involved in the fight against this beetle in an urban area.
Imaging Modality to Guide Left Atrial Appendage Closure: Current Status and Future Perspectives
Authors: Laterra Giulia , Dattilo Giuseppe , Correale Michele , Brunetti Natale Daniele , Artale Claudia , Sacchetta Giorgio , Pistelli Lorenzo , Borgi Marco , Campanella Francesca , Cocuzza Federica , Lo Nigro Maria Claudia , Contarini Marco
Published: May 2023

Atrial fibrillation (AF) is the most common cardiac arrhythmia in adults. The left atrial appendage (LAA) is the most likely source of thrombus formation in patients with non-valvular atrial fibrillation (NVAF). Left atrial appendage closure (LAAC) represents an effective alternative to NOAC in patients with NVAF. Expert consensus documents recommend intraprocedural imaging by means of either transesophageal echocardiography (TEE) or intracardiac echocardiography (ICE) in addition to standard fluoroscopy to guide LAAC. TEE-guided LAAC usually requires general anesthesia. The ICE technique is a “minimalist approach”, without general anesthesia, but ICE imaging techniques are not yet simplified and standardize, and the ICE may result in inferior image quality compared with that of TEE. Another “minimalist approach” can be the use of ICE via the esophageal route (ICE-TEE), that jet is validated to identify the presence of LAA thrombi in patients and to perform other procedures. In our cath laboratory ICE-TEE to guide LAAC is used in some complex patients. Indeed, our single center experience suggests that ICE-TEE could be a good alternative imaging technique to guide LAAC procedure without general anesthesia.
Functional L-Arginine Derivative as an Efficient Vector for Intracellular Protein Delivery for Potential Cancer Therapy
Authors: He Xiao , Qu Yannv , Xiong Su , Jiang Zhiru , Tang Yaqin , Yan Fei , Deng Yuanfei , Sun Yansun
Published: May 2023

The utilization of cytosolic protein delivery is a promising approach for treating various diseases by replacing dysfunctional proteins. Despite the development of various nanoparticle-based intracellular protein delivery methods, the complicated chemical synthesis of the vector, loading efficiency and endosomal escape efficiency of proteins remain a great challenge. Recently, 9-fluorenylmethyloxycarbonyl (Fmoc)-modified amino acid derivatives have been used to self-assemble into supramolecular nanomaterials for drug delivery. However, the instability of the Fmoc group in aqueous medium restricts its application. To address this issue, the Fmoc ligand neighboring arginine was substituted for dibenzocyclooctyne (DBCO) with a similar structure to Fmoc to obtain stable DBCO-functionalized L-arginine derivative (DR). Azide-modified triethylamine (crosslinker C) was combined with DR to construct self-assembled DRC via a click chemical reaction for delivering various proteins, such as BSA and saporin (SA), into the cytosol of cells. The hyaluronic-acid-coated DRC/SA was able to not only shield the cationic toxicity, but also enhance the intracellular delivery efficiency of proteins by targeting CD44 overexpression on the cell membrane. The DRC/SA/HA exhibited higher growth inhibition efficiency and lower IC50 compared to DRC/SA toward various cancer cell lines. In conclusion, DBCO-functionalized L-arginine derivative represents an excellent potential vector for protein-based cancer therapy.
Distribution and Polymorphisms of Group I Introns in Mitochondrial Genes from Cryptococcus neoformans and Cryptococcus gattii
Authors: Gomes Ronald Muryellison Oliveira da Silva , da Silva Kássia Jéssica Galdino , Ferreira Leonardo Capistrano , Arantes Thales Domingos , Theodoro Raquel Cordeiro
Published: May 2023

The species complexes Cryptococcus neoformans and Cryptococcus gattii are the causative agents of cryptococcosis. Virulence and susceptibility to antifungals may vary within each species according to the fungal genotype. Therefore, specific and easily accessible molecular markers are required to distinguish cryptic species and/or genotypes. Group I introns are potential markers for this purpose because they are polymorphic concerning their presence and sequence. Therefore, in this study, we evaluated the presence of group I introns in the mitochondrial genes cob and cox1 in different Cryptococcus isolates. Additionally, the origin, distribution, and evolution of these introns were investigated by phylogenetic analyses, including previously sequenced introns for the mtLSU gene. Approximately 80.5% of the 36 sequenced introns presented homing endonucleases, and phylogenetic analyses revealed that introns occupying the same insertion site form monophyletic clades. This suggests that they likely share a common ancestor that invaded the site prior to species divergence. There was only one case of heterologous invasion, probably through horizontal transfer to C. decagattii (VGIV genotype) from another fungal species. Our results showed that the C. neoformans complex has fewer introns compared to C. gattii. Additionally, there is significant polymorphism in the presence and size of these elements, both among and within genotypes. As a result, it is impossible to differentiate the cryptic species using a single intron. However, it was possible to differentiate among genotypes within each species complex, by combining PCRs of mtLSU and cox1 introns, for C. neoformans species, and mtLSU and cob introns for C. gattii species.