bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptome and Small RNA Sequencing Analysis Revealed Roles of PaWB-Related miRNAs and Genes in Paulownia fortunei
Authors: Li Bingbing , Zhai Xiaoqiao , Cao Yabing , Zhao Hui , Wang Zhe , Liu Haifang , Fan Guoqiang
Published: July 2018

Paulownia witches’ broom (PaWB) is an epidemic disease caused by phytoplasmas infection, which is responsible for large production and economic losses. The study of PaWB has made significant progress, but the specific molecular mechanisms associated with PaWB remain unclear. To clearly know the gene expression profiles of plantlets infected with phytoplasmas, in this study, we used high-throughput sequencing technology to generate an integrated analysis of the transcriptome and microRNAs (miRNAs) of Paulownia fortunei (seem.) Hemsl. plantlets, and to obtain a comprehensive resource for the relationship between vital miRNA-target gene pairs and PaWB. A total of 756 genes, and 45 conserved and 22 new miRNAs were identified associated with PaWB. In addition, 635 target genes were predicted for the 67 DERs (Differentially expressed miRNAs). An interaction network of these miRNAs and their target genes was constructed. Gene ontology (GO) and KEGG pathway analysis of these target genes indicated that genes encoding transcription factors (TFs), including auxin response factors (ARF), WRKY, NAC (NAM, ATAF1/2 and CUC2), and MYB (v-myb avian myeloblastosis viral oncogene homolog), and genes encoding superoxide dismutase (SOD), as well as alternative splicing were related directly or indirectly to PaWB. Our results shed light on the possible roles of genes and miRNAs in PaWB-infected plantlets, which will enhance the understanding of the PaWB mechanism in Paulownia plants.
Validation of a Methodology for Confidence-Based Participatory Forest Management
Authors: Martínez-Falero Eugenio , González-García Concepción , García-Abril Antonio , Ayuga-Téllez Esperanza
Published: July 2018

This paper formulates a new strategy for participatory forest management consisting of encouraging public participation as long as it increases empathy among participants. The strategy requires the homogeneous representation of the opinion of a participant (i.e., to determine how they assess a forest plan and identify the best one). Utility assessments are prepared for participants through pair-comparisons between meaningful points in the territory and from value functions based on forest indicators. The best plan is designed by applying combinatorial optimization algorithms to the utility of a participant. The calculating of empathy—of one participant relative to another—is based on the equivalence of their respective utilities when the current forest plan is modified. This involves calculating the opinions that are due to systematic changes in the collective plan for those participants that each participant supposes will affect the utility of the other participants. Calculating empathy also requires knowing the interactions among participants, which have been incorporated through agent-based simulation models. Application of the above methodology has confirmed the association between increases in empathy and convergence of opinions in different scenarios: well and medium-informed participants and with and without interaction among them, which verifies the proposed strategy. In addition, this strategy is easily integrated into available information systems and its outcomes show advantages over current participatory applications.
The Spatial-Temporal Characteristics and Influential Factors of NOx Emissions in China: A Spatial Econometric Analysis
Authors: Diao Beidi , Ding Lei , Su Panda , Cheng Jinhua
Published: July 2018

While the progress of China’s industrialization and urbanization has made great strides, atmospheric pollution has become the norm, with a wide range of influence and difficult governance. While many previous works on NOx pollution have been developed from the perspectives of natural science and technology, few studies have been conducted from social-economic points of view, and regional differences have not been given adequate attention in driving force models. This paper adopts China’s provincial panel data from 2006 to 2015, an extended STIRPAT (Stochastic Impacts by Regression on Population, Affluence and Technology) model, and spatial econometric models to investigate the socio-economic influential factors and spatial-temporal patterns of NOx emissions. According to the spatial correlation analysis results, the provincial NOx emission changes not only affected the provinces themselves, but also neighboring regions. Spatial econometric analysis shows that the spatial effect largely contributes to NOx emissions. The other explanatory variables all have positive impacts on NOx emissions, except for the vehicular indicator (which did not pass the significance test). As shown through the estimated consequences of direct and indirect effects, the indicators have significant positive effects on their own areas, and exacerbate NOx pollution. In terms of indirect effects, only three factors passed the significant test. An increase in gross domestic product (GDP) and energy consumption will exacerbate adjacent NOx pollution. Finally, a series of socio-economic measures and regional cooperation policies should be applied to improve the current air environment in China.
Data on Determinants Are Needed to Curb the Sedentary Epidemic in Europe. Lessons Learnt from the DEDIPAC European Knowledge Hub
Authors: De Craemer Marieke , Chastin Sebastien , Ahrens Wolfgang , Bernaards Claire , Brug Johannes , Buck Christoph , Cardon Greet , Capranica Laura , Dargent-Molina Patricia , De Lepeleere Sara , Hoffmann Belinda , Kennedy Aileen , Lakerveld Jeroen , Lien Nanna , Ling Fiona , Loyen Anne , MacDonncha Ciaran , Nazare Julie-Anne , O’Donoghue Grainne , O’Gorman Donal , Perchoux Camille , Pigeot Iris , Simon Chantal , Mueller-Stierlin Annabel S. , Van der Ploeg Hidde , Van Cauwenberg Jelle , Oppert Jean-Michel
Published: July 2018

Societal and technological changes have resulted in sitting being the dominant posture during most activities of daily living, such as learning, working, travelling and leisure time. Too much time spent in seated activities, referred to as sedentary behaviour, is a novel concern for public health as it is one of the key lifestyle causes of poor health. The European DEDIPAC (Determinants of Diet and Physical Activity) Knowledge Hub coordinated the work of 35 institutions across 12 European member states to investigate the determinants of sedentary behaviour. DEDIPAC reviewed current evidence, set a theoretical framework and harmonised the available epidemiological data. The main results are summarised. The conclusion is that there is a dire lack of data that is exploitable across Europe to inform policy and intervention. There is an urgent need to develop international data collection compliant with FAIR (Findable, Accessible, Interoperable, Re-usable) and standardised surveillance systems for sedentary behaviour.
C. elegans—An Emerging Model to Study Metal-Induced RAGE-Related Pathologies
Authors: Pinkas Adi , Cunha Martins Airton , Aschner Michael
Published: July 2018

The receptor for advanced glycation end products (RAGE), a multi-ligand receptor, is mostly associated with promoting inflammation and oxidative stress. In addition to advanced glycation end products (AGEs), its ligands include High mobility group box 1 protein (HMGB-1), S-100 proteins and beta-sheet fibrils. The effects of several metals and metalloids on RAGE expression and activation have been recently studied: in vivo and in vitro exposure to methylmercury, selenium, zinc, manganese, and arsenic was associated with a variety of RAGE-related alterations and behavioral impairments, which are mostly dependent upon the administration procedure (local vs. systemic) and age during exposure. Recently, C. elegans has been proposed as a potential novel model for studying RAGE-related pathologies; preliminary data regarding such model and its potential contribution to the study of metal-induced RAGE-related pathologies are discussed.
Gender Difference in the Association between Environmental Tobacco Smoke and Birth Weight in Africa
Authors: Owili Patrick Opiyo , Muga Miriam Adoyo , Kuo Hsien-Wen
Published: July 2018

The adverse health effects of exposure to environmental tobacco smoke (ETS) on children are well-documented, and yet, gender difference in low birthweight among newborns whose mothers were exposed to ETS during pregnancy still remains contentious. We therefore explored the association between ETS exposure and risk of low birthweight, and further determined the gender difference in the association between exposure to ETS during pregnancy and birth weight in Africa. The Demographic Health Surveys of 23 African countries with information on 208,027 newborns were used. The associations between exposure to ETS and birth weight was estimated using multiple logistic regression models. Exposure to ETS increased the risk of low birthweight in Africa (adjusted odds ratio (OR) = 1.06; 95% Confidence Interval (CI): 1.02–1.10). A stratified analysis, by gender, revealed that male newborns whose mothers were exposed to ETS were 1.08 (95% CI: 1.02–1.14) times more likely to be low in birthweight than those whose mothers were not exposed, with those exposed weekly (adjusted OR = 1.17; 95% CI: 1.01–1.35) and daily (adjusted OR = 1.06; 95% CI: 1.01–1.12) being more likely to have low birthweight. Exposure to ETS is significantly associated with low birthweight in Africa, mainly among male newborns. Gender could possibly be a modifier, and hence, research on biological plausibility is necessary. Moreover, a public health promotion on behavioral changes is likely to have a positive impact on newborns’ health.
Misophonia in Singaporean Psychiatric Patients: A Cross-Sectional Study
Authors: Quek Tian Ci , Ho Cyrus SH. , Choo Carol C. , Nguyen Long H. , Tran Bach X. , Ho Roger C.
Published: July 2018

Misophonia, also known as selective sound sensitivity syndrome, is a condition characterized by strong dislike of specific sounds with accompanying distressing reactions. To date, misophonia is still poorly understood. This study aimed to identify factors associated with severity of misophonic symptoms in Singaporean psychiatric patients. Ninety-two psychiatric patients were recruited from a large teaching hospital in Singapore in a cross-sectional study. Socio-demographics, severity of depression, anxiety and stress, and severity of misophonic symptoms were analyzed. Correlation analysis showed that anxiety, depression, and stress scores—as measured by the Depression, Anxiety and Stress Scales-21 (DASS-21)—were significantly positively correlated with the Amsterdam Misophonia Scale (A-MISO-S) scores. After adjustment for confounding factors, multivariate regression analysis showed that anxiety (β = 0.385, p = 0.029) remained significantly associated with A-MISO-S. Age, gender, depression, and stress were not significantly associated with the severity of misophonia. The findings showed that the severity of anxiety was associated with severity of misophonia in Singaporean psychiatric patients. Further research is needed to explore the nature of misophonia and its relationship with other psychiatric disorders.
Weight-Dependent Disparities in Adolescent Girls: The Impact of a Brief Pilot Intervention on Exercise and Healthy Eater Identity
Authors: Kramer Eydie N. , Chard Christine A. , Walters Kellie , Barr-Anderson Daheia J.
Published: July 2018

Adolescent girls report low participation in healthy behaviors (e.g., nutritious eating and exercise), and are disproportionately affected by obesity. Short-term interventions, such as behavioral summer camps, may positively influence psychological underpinnings of healthy behavior, particularly exercise identity (EI) and healthy eater identity (HEI). The present study investigates disparities and changes in identity and subsequent health behavior in two cohorts of adolescent girls following a brief, multicomponent intervention. A sample of normal-weight adolescent girls from a health promotion camp and an elevated body mass index (BMI) sample from an obesity treatment camp participated in the study. Both camps ran one-week in duration and delivered comparable intervention components. All families were given access to the same eight-week eHealth program post-camp. Significant EI and HEI role-identity disparities between the health promotion and obesity treatment cohorts were apparent at baseline. Following the one-week camp intervention, EI and HEI scores increased in both groups. At follow-up, the treatment group had increased EI and HEI role-identities in such that the groups no longer significantly differed. Positive changes in health behaviors were experienced in each group. This pilot study demonstrates that EI and HEI differ between normal-weight and obese adolescent girls and weight-dependent identity disparities may be mitigated following brief, multicomponent interventions.
Serum Uric Acid in Roma and Non-Roma—Its Correlation with Metabolic Syndrome and Other Variables
Authors: Petrikova Jana , Janicko Martin , Fedacko Jan , Drazilova Sylvia , Madarasova Geckova Andrea , Marekova Maria , Pella Daniel , Jarcuska Peter
Published: July 2018

Background: The Roma population is one of the major marginalized groups in Europe, having higher incidence of all spectrums of disease and a shorter life expectancy. Yet, the reasons for higher morbidity and its exact prevalence were not properly studied. Objectives: The objective of our study was to compare the frequency of metabolic syndrome (MetS) in Roma people to the non-Roma population in Slovakia, and to compare levels of uric acid and its correlation with components of metabolic syndrome. Methods: A group of 452 Roma people aged 18–55 years, was compared to a control group of 403 non-Roma people. The data were obtained by questionnaire, anthropometric measures, and analyzed blood and urine samples Results: The prevalence of MetS was significantly higher among Roma participants (131; 29.6%) compared with non-Roma participants (80; 20.1%), p = 0.001. Roma people significantly more often fulfilled obesity and low high-density lipoprotein (HDL) criteria of MetS (257, 58.9% vs. 180, 45.8%, p < 0.0001, and 312, 70.0% vs. 140, 34.9%, p < 0.0001). There was no difference in the triacylglycerols (TG), glycemia or blood pressure (BP) criteria of MetS. The Roma also presented with greater levels of high sensitivity C-reactive protein (hs-CRP). Baseline levels of uric acid (UA) among the Roma population were significantly lower compared with the majority population (226.54 ± 79.8 vs. 259.11 ± 84.53) (p < 0.001). The levels of UA significantly correlated with fulfilled criteria of MetS. Univariate regression showed that UA is a significant predictor of MetS in the whole cohort (unadjusted odds ratio (OR) 1.005; 95% CI 1.004–1.007; p < 0.0001) also after the adjustment for age, sex, and ethnicity (adjusted OR 1.008; 95% CI 1.005–1.010; p < 0.0001). Conclusions: We were able to show that prevalence of MetS among the Roma is higher than in the majority population. Moreover, the uric acid levels are significantly lower in the Roma group as well as when it comes to a cohort with MetS. Levels of UA, besides others, depend on ethnicity, age, and sex.
Targeting Macrophages as a Potential Therapeutic Intervention: Impact on Inflammatory Diseases and Cancer
Authors: Ponzoni Mirco , Pastorino Fabio , Di Paolo Daniela , Perri Patrizia , Brignole Chiara
Published: July 2018

Macrophages, cells belonging to the innate immune system, present a high plasticity grade, being able to change their phenotype in response to environmental stimuli. They play central roles during development, homeostatic tissue processes, tissue repair, and immunity. Furthermore, it is recognized that macrophages are involved in chronic inflammation and that they play central roles in inflammatory diseases and cancer. Due to their large involvement in the pathogenesis of several types of human diseases, macrophages are considered to be relevant therapeutic targets. Nanotechnology-based systems have attracted a lot of attention in this field, gaining a pivotal role as useful moieties to target macrophages in diseased tissues. Among the different approaches that can target macrophages, the most radical is represented by their depletion, commonly obtained by means of clodronate-containing liposomal formulations and/or depleting antibodies. These strategies have produced encouraging results in experimental mouse models. In this review, we focus on macrophage targeting, based on the results so far obtained in preclinical models of inflammatory diseases and cancer. Pros and cons of these therapeutic interventions will be highlighted.
Hamster Melatonin Receptors: Cloning and Binding Characterization of MT1 and Attempt to Clone MT2
Authors: Gautier Célia , Dufour Emilie , Dupré Clémence , Lizzo Giulia , Caignard Sarah , Riest-Fery Isabelle , Brasseur Chantal , Legros Céline , Delagrange Philippe , Nosjean Olivier , Simonneaux Valérie , Boutin Jean A. , Guenin Sophie-Pénélope
Published: July 2018

For many years, it was of interest to identify the sequences encoding the two melatonin receptors (MT1 and MT2) from various species. After publishing the basic molecular characterization of the human, rat, mouse, sheep, and platypus MT1, MT2, or Mel1c receptors, we began cloning the genes from other animals, such as birds, bats, and vipers. The goal was to advance the receptor crystallization, which could greatly contribute the understanding of the sequence/stability relationship. European hamster MT1 receptor was cloned for the first time from this gender, was expressed in stable form in cells, and its binding characterized with a sample of 19 melatonin ligands. Siberian hamster (Phodopus sungorus) expresses a non-functional MT2. We observed that unlike this hamster, the European hamster (Cricetus cricetus) does not have a stop codon in the MT2 sequence. Thus, we undertook the tedious task of cloning the MT2 receptor. We partially succeeded, sequencing the complete exon 2 and a fragment of exon 1 (from putative amino acids 12 to 38 and 77 to 323), after several years of efforts. In order to show that the protein parts we cloned were capable to sustain some binding capacities, we designed a chimeric MT2 receptor using a consensus sequence to replace the unknown amino acids, based on other small rodent MT2 sequences. This chimeric construct could bind melatonin in the nanomolar range. This work is meant to be the basis for attempts from other laboratories of the community to determine the complete natural sequence of the European hamster MT2 receptor. The present work is the first to show that, among the hamsters, if the Siberian is a natural knockout for MT2, the European one is not.
Anti-Proliferative Properties and Proapoptotic Function of New CB2 Selective Cannabinoid Receptor Agonist in Jurkat Leukemia Cells
Authors: Capozzi Antonella , Mattei Vincenzo , Martellucci Stefano , Manganelli Valeria , Saccomanni Giuseppe , Garofalo Tina , Sorice Maurizio , Manera Clementina , Misasi Roberta
Published: July 2018

Several studies demonstrated that cannabinoids reduce tumor growth, inhibit angiogenesis, and decrease cancer cell migration. As these molecules are well tolerated, it would be interesting to investigate the potential benefit of newly synthesized compounds, binding cannabinoid receptors (CBRs). In this study, we describe the synthesis and biological effect of 2-oxo-1,8-naphthyridine-3-carboxamide derivative LV50, a new compound with high CB2 receptor (CB2R) affinity. We demonstrated that it decreases viability of Jurkat leukemia cells, evaluated by Trypan Blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), but mainly induces a proapoptotic effect. We observed an increase of a hypodiploid peak by propidium iodide staining and changes in nuclear morphology by Hoechst 33258. These data were confirmed by a significant increase of Annexin V staining, cleavage of the nuclear enzyme poly(ADP-ribose)-polymerase (PARP), and caspases activation. In addition, in order to exclude that LV50 non-specifically triggers death of all normal leukocytes, we tested the new compound on normal peripheral blood lymphocytes, excluding the idea of general cytotoxicity. To characterize the involvement of CB2R in the anti-proliferative and proapoptotic effect of LV50, cells were pretreated with a specific CB2R antagonist and the obtained data showed reverse results. Thus, we suggest a link between inhibition of cell survival and proapoptotic activity of the new compound that elicits this effect as selective CB2R agonist.
Verapamil Inhibits TRESK (K2P18.1) Current in Trigeminal Ganglion Neurons Independently of the Blockade of Ca2+ Influx
Authors: Park Hyun , Kim Eun-Jin , Ryu Ji , Lee Dong , Hong Seong-Geun , Han Jaehee , Han Jongwoo , Kang Dawon
Published: July 2018

Tandem pore domain weak inward rectifier potassium channel (TWIK)-related spinal cord K+ (TRESK; K2P18.1) channel is the only member of the two-pore domain K+ (K2P) channel family that is activated by an increase in intracellular Ca2+ concentration ([Ca2+]i) and linked to migraines. This study was performed to identify the effect of verapamil, which is an L-type Ca2+ channel blocker and a prophylaxis for migraines, on the TRESK channel in trigeminal ganglion (TG) neurons, as well as in a heterologous system. Single-channel and whole-cell currents were recorded in TG neurons and HEK-293 cells transfected with mTRESK using patch-clamping techniques. In TG neurons, changes in [Ca2+]i were measured using the fluo-3-AM Ca2+ indicator. Verapamil, nifedipine, and NiCl2 inhibited the whole-cell currents in HEK-293 cells overexpressing mTRESK with IC50 values of 5.2, 54.3, and >100 μM, respectively. The inhibitory effect of verapamil on TRESK channel was also observed in excised patches. In TG neurons, verapamil (10 μM) inhibited TRESK channel activity by approximately 76%. The TRESK channel activity was not dependent on the presence of extracellular Ca2+. In addition, the inhibitory effect of verapamil on the TRESK channel remained despite the absence of extracellular Ca2+. These findings show that verapamil inhibits the TRESK current independently of the blockade of Ca2+ influx in TG neurons. Verapamil will be able to exert its pharmacological effects by modulating TRESK, as well as Ca2+ influx, in TG neurons in vitro. We suggest that verapamil could be used as an inhibitor for identifying TRESK channel in TG neurons.
Microstructure and Mechanical Properties of Butt Joints between Stainless Steel SUS304L and Aluminum Alloy A6061-T6 by TIG Welding
Authors: Nguyen Van Nhat , Nguyen Quoc Manh , Huang Shyh-Chour
Published: July 2018

The tungsten inert gas (TIG) welding method most commonly used to weld ferrous metals, nonferrous metals, and other metals since it is simple, easily implemented, and achieves consistent high-quality welds. In this study, butt joints produced between aluminum alloy A6061-T6 and stainless steel SUS304L have been achieved by using TIG welding with ER4047 filler metal. The macrostructure and microstructure of the resulting specimens were analyzed by means of an optical microscope (OM), a scanning electron microscope (SEM), and an energy dispersive X-ray spectrometer (EDS). A uniform intermetallic layer was found at the interface between the stainless steel and the weld seam having a thickness of 2 µm, and the intermetallic compound (IMC) includes Fe4Al13, Fe2Al5, and FeAl3 phases. The micro-hardness and tensile strength of the weld joints were also investigated. Due to Si content in the compensating metal, there was a prevention of iron diffusion into the aluminum, thus hindering the development of the IMC layer and reducing its thickness in such a way that the weld joint strength increases. The analyzed results show that the average micro-hardness of the stainless steel, weld seam, aluminum alloys, and IMC layer were 218 HV, 88.3 HV, 63.3 HV, and 411 HV, respectively. The fracture occurred at the brazed interface, and the ultimate tensile strength value reached 225 MPa.
Sorbicillinoid-Based Metabolites from a Sponge-Derived Fungus Trichoderma saturnisporum
Authors: Meng Junjun , Cheng Wei , Heydari Hajar , Wang Bin , Zhu Kui , Konuklugil Belma , Lin Wenhan
Published: July 2018

Antibacterial activity assessment and high performance liquid chromatography associated with nuclear magnetic resonance (HPLC/NMR) data revealed that the EtOAc extract of the fermented endophytic fungus Trichoderma saturnisporum DI-IA, obtained from the marine sponge Dictyonella incisa, contained conjugated olefinic metabolites with antibacterial activity. Chemical examination of the fungal strain resulted in the isolation of eight new sorbicillinoid-based compounds, namely saturnispols A–H (1–8). Their structures were determined on the basis of extensive spectroscopic analysis, including the experimental and calculated electronic circular dichroism (ECD) data for the configurational assignments. Saturnispol F exerted significant inhibition against a panel of bacteria strains including vancomycin-resistant enterococci (VRE) with a minimum inhibitory concentrations (MIC) ranging from 1.63 to 12.9 μg/mL, while saturnispol H showed selective effects against VRE and B. subtilis.
Harnessing Marine Biocatalytic Reservoirs for Green Chemistry Applications through Metagenomic Technologies
Authors: Castilla Ignacio Abreu , Woods David F. , Reen F. Jerry , O’Gara Fergal
Published: July 2018

In a demanding commercial world, large-scale chemical processes have been widely utilised to satisfy consumer related needs. Chemical industries are key to promoting economic growth and meeting the requirements of a sustainable industrialised society. The market need for diverse commodities produced by the chemical industry is rapidly expanding globally. Accompanying this demand is an increased threat to the environment and to human health, due to waste produced by increased industrial production. This increased demand has underscored the necessity to increase reaction efficiencies, in order to reduce costs and increase profits. The discovery of novel biocatalysts is a key method aimed at combating these difficulties. Metagenomic technology, as a tool for uncovering novel biocatalysts, has great potential and applicability and has already delivered many successful achievements. In this review we discuss, recent developments and achievements in the field of biocatalysis. We highlight how green chemistry principles through the application of biocatalysis, can be successfully promoted and implemented in various industrial sectors. In addition, we demonstrate how two novel lipases/esterases were mined from the marine environment by metagenomic analysis. Collectively these improvements can result in increased efficiency, decreased energy consumption, reduced waste and cost savings for the chemical industry.
Discrimination of Aroma Characteristics for Cubeb Berries by Sensomics Approach with Chemometrics
Authors: Cheng Huan , Chen Jianle , Watkins Peter J. , Chen Shiguo , Wu Dan , Liu Donghong , Ye Xingqian
Published: July 2018

The dried cubeb berries are widely used as medicinal herb and spicy condiment with special flavor. However, there is a significant definition discrepancy for cubeb berries. In this study, an efficient analytical method to characterize and discriminate two popular cubeb fruits (Litsea cubeba and Piper cubeba) was established. The aroma profiles of cubeb berries were evaluated by different extraction methods including hydro-distillation, simultaneous distillation/extraction, and solid-phase micro-extraction followed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). In total, 90 volatile compounds were identified by HD, SDE, and SPME combined with GC-MS. Principal component analysis was further applied and discriminated ambiguous cubeb berries by their unique aromas: Litsea cubeba was characterized by higher level of d-limonene (“fruit, citrus”), citral (“fruit, lemon”) and dodecanoic acid; α-cubebene (“herb”) was identified as a marker compound for Piper cubeba with higher camphor (“camphoraceous”), and linalool (“flower”). Flavor fingerprint combined with PCA could be applied as a promising method for identification of cubeb fruits and quality control for food and medicinal industries.
Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
Authors: Wu Yi-Feng , Zheng Hong-Bo , Liu Xin-Yan , Cheng Ai-Xia , Lou Hong-Xiang
Published: July 2018

Alkenal double bond reductases (DBRs), capable of catalyzing the NADPH-dependent reduction of the α,β-unsaturated double bond, play key roles in the detoxication of alkenal carbonyls. Here, the isolation and characterization of two DBRs encoded by the liverwort species Marchantia paleacea are described. The two DBRs share a relatively low similarity, and phylogenetic analysis indicated that MpMDBRL is more closely related to microbial DBRs than to other plant DBRs, while MpDBR shares common ancestry with typical plant DBRs. Both DBR proteins exhibited hydrogenation ability towards hydroxycinnamyl aldehydes; however, their temperature optimums were strikingly different. MpMDBRL demonstrated slightly weaker catalytic efficiency compared to MpDBR, and the structural models of their active binding sites to the substrate may provide a parsimonious explanation. Furthermore, both DBRs significantly responded to phytohormone treatment. In conclusion, M. paleacea produces two distinct types of functional DBRs, both of which participate in the protection against environmental stress in liverwort. The presence of a microbial type of DBR in a plant is herein reported for the first time.
Liquid Chromatography Coupled with Linear Ion Trap Hybrid OrbitrapMass Spectrometry for Determination of Alkaloids in Sinomeniumacutum
Authors: Shan Jinjun , Zhao Xia , Shen Cunsi , Ji Jianjian , Xu Jianya , Wang Shouchuan , Xie Tong , Tong Wenjun
Published: July 2018

The characterization of alkaloids is challenging because of the diversity of structures and the complicated fragmentation of collision induced structural dissociation in mass spectrometry. In this study, we analyzed the alkaloids in Sinomenium acutum (Thunb.) Rehderet Wil by high resolution mass spectrometry. Chromatographic separation was achieved on a Phenomenex Kinetex C18 (2.1 mm × 100 mm, 2.6 μm) column with a mobile phase consisting of acetonitrile and water (0.1% formic acid) under gradient elution. A total of 52 alkaloids were well separated and 45 of them were structurally characterized, including morphinans, aporphines, benzylisoquinolines, and protoberberines. Specially, mass spectrometric study of the morphinan alkaloids were explicitly investigated. Electrostatic potential plot from simulation was calculated for determination of protonation sites. Further fragmentation analysis suggested that the C3H7N, CH4O, and H2O elimination was displayed in MS2 spectrum. These fragmentation pathways are universal for morphinan alkaloids having methoxy substituted cyclohexenone or cyclohexadienone moieties. Additionally, for nitrogen oxides, an ion-neutral complex intermediate is involved in the fragmentation process, generating additional oxygenated ions. All these results provided the universal rules of fragmentation used for detection of alkaloids, and will be expected to be highly useful for comprehensive study of multi-components in the herbal medicine analysis.
NG2 Glia: Novel Roles beyond Re-/Myelination
Authors: Parolisi Roberta , Boda Enrica
Published: July 2018

Neuron-glia antigen 2-expressing glial cells (NG2 glia) serve as oligodendrocyte progenitors during development and adulthood. However, recent studies have shown that these cells represent not only a transitional stage along the oligodendroglial lineage, but also constitute a specific cell type endowed with typical properties and functions. Namely, NG2 glia (or subsets of NG2 glia) establish physical and functional interactions with neurons and other central nervous system (CNS) cell types, that allow them to constantly monitor the surrounding neuropil. In addition to operating as sensors, NG2 glia have features that are expected for active modulators of neuronal activity, including the expression and release of a battery of neuromodulatory and neuroprotective factors. Consistently, cell ablation strategies targeting NG2 glia demonstrate that, beyond their role in myelination, these cells contribute to CNS homeostasis and development. In this review, we summarize and discuss the advancements achieved over recent years toward the understanding of such functions, and propose novel approaches for further investigations aimed at elucidating the multifaceted roles of NG2 glia.
Dietary Intake, Body Composition, and Oral Health Parameters among Female Patients with Primary Sjögren’s Syndrome
Authors: Nesvold Marianne B. , Jensen Janicke L. , Hove Lene H. , Singh Preet B. , Young Alix , Palm Øyvind , Frost Andersen Lene , Carlsen Monica H. , Iversen Per Ole
Published: July 2018

There is limited knowledge about dietary intake and body composition among patients with primary Sjögren’s syndrome. We assessed dietary intakes with 24-h recalls and body composition with anthropometry and bioelectrical impedance in 20 female patients. Various scoring tools were used to assess oral health. The patients had a lower energy percentage (E%) from carbohydrates (p = 0.02) and a higher E% from fat (p = 0.01) compared to a reference group. The lower intake of carbohydrates was due to a lower bread intake (p = 0.04), while the higher intake of fat was due to a higher intake of butter, margarine, and oil (p = 0.01). The patients ate more than twice (p = 0.02) as much fish as the reference group. The compliance to recommended intakes of macro- and micronutrients was good. Forty-percent of the patients were overweight/obese. Increased intake of beverages was observed in patients with severe xerostomia and/or low oral health-related quality of life, whereas reduced fat intake was found in hyposmic patients. In conclusion, the dietary intake among the patients was not much different from the reference group and complied with recommendations. Most oral health parameters were not associated with nutrient intakes. Specific dietary guidelines are probably not needed to ensure adequate nutrition among such patients.
Design, Synthesis, In Vitro, and Initial In Vivo Evaluation of Heterobivalent Peptidic Ligands Targeting Both NPY(Y1)- and GRP-Receptors—An Improvement for Breast Cancer Imaging?
Authors: Vall-Sagarra Alicia , Litau Shanna , Decristoforo Clemens , Wängler Björn , Schirrmacher Ralf , Fricker Gert , Wängler Carmen
Published: July 2018

Heterobivalent peptidic ligands (HBPLs), designed to address two different receptors independently, are highly promising tumor imaging agents. For example, breast cancer has been shown to concomitantly and complementarily overexpress the neuropeptide Y receptor subtype 1 (NPY(Y1)R) as well as the gastrin-releasing peptide receptor (GRPR). Thus, radiolabeled HBPLs being able to bind these two receptors should exhibit an improved tumor targeting efficiency compared to monospecific ligands. We developed here such bispecific HBPLs and radiolabeled them with 68Ga, achieving high radiochemical yields, purities, and molar activities. We evaluated the HBPLs and their monospecific reference peptides in vitro regarding stability and uptake into different breast cancer cell lines and found that the 68Ga-HBPLs were efficiently taken up via the GRPR. We also performed in vivo PET/CT imaging and ex vivo biodistribution studies in T-47D tumor-bearing mice for the most promising 68Ga-HBPL and compared the results to those obtained for its scrambled analogs. The tumors could easily be visualized by the newly developed 68Ga-HBPL and considerably higher tumor uptakes and tumor-to-background ratios were obtained compared to the scrambled analogs in and ex vivo. These results demonstrate the general feasibility of the approach to use bispecific radioligands for in vivo imaging of breast cancer.
Recent Overviews in Functional Polymer Composites for Biomedical Applications
Authors: Zagho Moustafa , Hussein Essraa , Elzatahry Ahmed
Published: July 2018

Composite materials are considered as an essential part of our daily life due to their outstanding properties and diverse applications. Polymer composites are a widespread class of composites, characterized by low cost, facile processing methods, and varied applications ranging from daily-use issues to highly complicated electronics and advanced medical combinations. In this review, we focus on the most important fabrication techniques for bioapplied polymer composites such as electrospinning, melt-extrusion, solution mixing, and latex technology, as well as in situ methods. Additionally, significant and recent advances in biomedical applications are spotlighted, such as tissue engineering (including bone, blood vessels, oral tissues, and skin), dental resin-based composites, and wound dressing.
Estimation of Lake Outflow from the Poorly Gauged Lake Tana (Ethiopia) Using Satellite Remote Sensing Data
Authors: Duan Zheng , Gao Hongkai , Ke Changqing
Published: July 2018

Lake Tana is the largest lake in Ethiopia, and its lake outflow is the source of the Blue Nile River that provides vital water resources for many livelihoods and downstream/international stakeholders. Therefore, it is essential to quantify and monitor the water balance of Lake Tana. However, Lake Tana is poorly gauged, with more than 50% of Lake Tana Basin being ungauged from in-situ measurements, making it difficult to quantify the lake inflow from surrounding basins. The lack of in-situ measurements highlights the need for the innovative application of satellite remote sensing. This study explores how freely accessible satellite remote sensing can be used to complement routine weather data to quantify the water balance of Lake Tana and its surrounding catchments. This study particularly investigates whether the outflow from Lake Tana can be estimated with sufficient accuracy as the residual of the lake water balance. Monthly inflow into lake was computed as the total runoff from the surrounding catchments; the runoff was estimated as the residual of the land-based catchment water balance using satellite precipitation improved with an integrated downscaling-calibration procedure, satellite evapotranspiration, and a correction term for changes in land total storage (soil moisture storage and deep percolation). The outflow from Lake Tana was estimated as the residual of lake water balance by combining satellite-based lake precipitation, changes in water storage, and lake inflow with estimated lake evaporation. Evaluation using limited available measurements showed that estimated annual runoff for two gauged subbasins agreed well with measurements, with differences within 4%. The estimated annual outflow from Lake Tana was also close to measured outflow, with a difference of 12%. However, the estimated monthly runoff from catchments and monthly lake outflow were unsatisfactory, with large errors.
A Generalized Logistic-Gaussian-Complex Signal Model for the Restoration of Canopy SWIR Hyperspectral Reflectance
Authors: Lin Chinsu
Published: July 2018

The continuum of the SWIR (short-wave infrared) signals from 1320 to 1650 nm contains valuable information for effectively diagnosing water, chlorophyll, and nitrogen content. The SWIR spectra of in situ spectroradiometric data and airborne spectrometric images are frequently contaminated by significant noise. Based on a Logistic-Gaussian complex signal model (LGCM), the noise-free signals at 1330–1349 and 1411–1430 nm wavelengths can provide critical bases for restoring the 1350–1410 nm wavelength signals for a single point of data. This paper proposes a generalized LGCM (GLGCM) technique to expand the ability of LGCM to process large data with variant reflectance values. A 12-year-old red cypress plantation located in a central Taiwan temperate forest was selected for this study. Hundreds of reflectance spectra of tree crowns were obtained using an ASD FR Spectroradiometer. The in-laboratory blank test showed that the GLGCM technique was able to achieve sufficient performance with an RMSE (root mean square error) of 0.0015 ± 0.0005 and 0.0011 ± 0.0005 for the front-edge and end-edge signal bases respectively, and 0.0014 ± 0.0006 in between the two signal bases. A significant level of noise between −0.2 and 0.4 was successfully removed from the in situ contaminated reflectance in the 1350–1410 nm wavelengths. The estimation bias for the signals of front-edge and end-edge bases was low, averaging 0.0031 ± 0.0003 and 0.0032 ± 0.0012. The consistency between the blank test and the in situ experimental results indicates that the GLGCM technique has potential in using batch processing to fix the problem of the noisy SWIR spectra in spectroradiometeric data and also airborne spectrometric images.
Millimeter Wave High Resolution Radar Accuracy in Fog Conditions—Theory and Experimental Verification
Authors: Golovachev Yosef , Etinger Ariel , Pinhasi Gad A. , Pinhasi Yosef
Published: July 2018

Attenuation and group delay effects on millimeter wave (MMW) propagation in clouds and fog are studied theoretically and verified experimentally using high resolution radar in an indoor space filled with artificial fog. In the theoretical analysis, the frequency-dependent attenuation and group delay were derived via the permittivity of the medium. The results are applied to modify the millimeter-wave propagation model (MPM) and employed to study the effect of fog and cloud on the accuracy of the Frequency-Modulated Continuous-Wave (FMCW) radar operating in millimeter wavelengths. Artificial fog was generated in the experimental study to demonstrate ultra-low visibility in a confined space. The resulted attenuation and group delay were measured using FMCW radar operating at 320–330 GHz. It was found that apart from the attenuation, the incremental group delay caused by the fog also played a role in the accuracy of the radar. The results were compared to the analytical model. It was shown that although the artificial fog has slight different characteristics compare to the natural fog and clouds, in particle composition, size, and density, the model predictions were good, pointing out that the dispersive effects should be considered in the design of remote sensing radars operating in millimeter and sub-millimeter wavelengths.
A Tightly Coupled RTK/INS Algorithm with Ambiguity Resolution in the Position Domain for Ground Vehicles in Harsh Urban Environments
Authors: Li Wei , Li Wenyi , Cui Xiaowei , Zhao Sihao , Lu Mingquan
Published: July 2018

Vehicles driving in urban canyons are always confronted with a degraded Global Navigation Satellite System (GNSS) signal environment. The surrounding obstacles may cause signal reflections or blockages, which lead to large multipath noises and intermittent GNSS reception. Under these circumstances, it is not feasible to use conventional real-time kinematic (RTK) algorithms to maintain high-precision performance for positioning. In order to meet the special requirements of safety-critical applications under non-ideal observation conditions, a novel tightly coupled RTK/Inertial Navigation System (INS) algorithm is proposed in this paper, which can provide accurate and reliable positioning results continuously. Our integrated RTK/INS algorithm has three features. Firstly, INS measurements are used to help search for integer ambiguities in the position domain. INS solutions can provide a more accurate initial location and a more efficient search region. Secondly, the criterion for determining whether a candidate position is the correct solution is only related to the fractional value of the carrier-phase measurement. Thus, the new algorithm is immune to cycle slips as well as large pseudorange noises. Thirdly, our algorithm can provide more accurate ranging information than the pseudorange, even though it may not necessarily be fixed successfully, because it selects the weighted ambiguity solution as the result rather than the candidate point with maximum probability. The proposed algorithm is demonstrated on both simulated and real datasets. Compared with single epoch RTK and conventional tightly coupled RTK/INS integrations that search integer ambiguities in the ambiguity domain, our method attains better accuracy and stability in a simulated environment. Moreover, the real experimental results are presented to validate the performance of the new integrated navigation algorithm.
Social-Spatial Accessibility to Urban Educational Resources under the School District System: A Case Study of Public Primary Schools in Nanjing, China
Authors: Xu Yan , Song Weixuan , Liu Chunhui
Published: July 2018

Recent discussions on the spatial distribution of educational resources center on exploring the social-spatial equity of the allocation of educational resources in the city. Following the research about spatial accessibility of urban educational resources, and based on the data about public primary school districts and residential districts in Nanjing, China, we propose a social-spatial accessibility computing framework to measure different degrees of accessibility to different public primary schools across geographic, opportunity, and economic dimensions. Results show that: (1) the geographic accessibility to schools in the inner-city is superior to those in the outer-city, but the spatial differences are rapidly shrinking as schools are being incorporated in the inner-city and built in the outer-city; (2) the opportunity accessibility to schools in the inner-city is inferior to those in the outer-city, and the access to the educational resources in the inner-city, especially high-quality education resources, becomes more scarce, due to the demolition in the inner-city and the construction in the outer-city; (3) economic accessibility is associated with the grade of the school, which, in the top school district, is the minimum, while there is a large decline in economic accessibility, with the overall soaring housing price in Nanjing. In other words, family background differences appear to have a significant influence on accessibility to high-quality educational resources (HQER). As a result, misallocation of educational resources, especially HQER, restrict social mobility and further exacerbate urban socio-spatial differentiation.
Understanding the Stakeholders’ Involvement in Utilizing Municipal Solid Waste in Agriculture through Composting: A Case Study of Hanoi, Vietnam
Authors: Le Nam Phong , Nguyen Thi Thu Phuong , Zhu Dajian
Published: July 2018

Although the involvement of stakeholders is believed to be the key to the success of sustainable municipal waste management, the specific features of stakeholders, as well as their interdependence, have been under-researched. This study employed a multilevel governance approach to understand the manner in which different types of stakeholder networks interact with one another and how their roles should be reinforced. A combination of stakeholder analysis (SA) and social network analysis (SNA) was employed to investigate the perspective of stakeholders in utilizing municipal solid waste (MSW) in agriculture in Hanoi, Vietnam. SA indicated that the local authorities take the main responsibility for the management of MSW in Hanoi. Although other stakeholders express a significant interest in recycling MSW through composting, many of them do not have sufficient power to make any changes to the current system. SNA revealed the fragmentation of the network, as the coordination among the stakeholders is dominated by hierarchical governance, while there is a lack of horizontal cooperation among the sectors. The fragmentation could be attributed to weak legislative framework, lack of trust, financial constraints, and the limited participation of private enterprises. The governance of MSW use in agriculture should be executed through interdependency rather than hierarchy and through a network comprising both state and non-state actors.