bluuebunny/crossref_metadata_2025 huggingface dataset:
A GIS-Based Hydrogeological Approach to the Assessment of the Groundwater Circulation in the Ischia Volcanic Island (Italy)
Authors: Fabbrocino Silvia , Bellucci Sessa Eliana , de Vita Sandro , Di Vito Mauro Antonio , Avino Rosario , Marotta Enrica
Published: August 2022

Assessing the variations in space and time of groundwater circulation in volcanic islands is of paramount importance to the description of the hydro-geo-thermal system and implementation of hydrogeological, geochemical, and volcanic monitoring systems. In fact, the reliable reconstruction of the groundwater potentiometric surface in such composite volcanic aquifer systems can enable the identification of the most advantageous strategies for both the sustainable use of groundwater resources and the management of volcanic risk. Geographical Information System (GIS) platforms can support the integration and analysis of many spatial and temporal variables derived from monitoring of active volcanoes and the elaboration of spatially continuous data. However, open issues still affect the reliability and general applicability of common spatial interpolation methods in the case of groundwater potentiometric surfaces. This is related to the assessment of the main stratigraphic and volcano-tectonic features affecting the hydraulic head changes. With regard to the dynamically very active Ischia Island (Italy), this study illustrates a GIS-based hydrogeological approach to identify the most accurate interpolation method for mapping the potentiometric surface in complex hydrogeological terrains. The proposed approach has been applied to the existing dataset (1977–2003) stored by Istituto Nazionale di Geofisica e Vulcanologia. Based on a careful geological and hydrogeological survey, a total of 267 wells, from 5 to 250 m in depth, were processed. The data pre-processing involved four meteorological time-series data (1922–1997) and six long records of piezometric water levels (1930–1994). As a result, knowledge of the delineation of rather homogeneous stratigraphic and volcano-tectonic structures at the basin-scale has improved. Thus, new, more reliable potentiometric surfaces of the four main geothermal areas closest to the coast were produced during both dry and wet seasons. The reliability of the processed potentiometric surface was then validated by comparing the spatially continuous data with complementary field data. These findings point toward an optimal interpolation approach for representing the seasonal and areal distribution of main hydrogeological parameters in complex aquifer systems. Finally, insights into variations of hydrological behavior at an active volcanic area will foster an understanding of possible involvement of fresh and thermal waters in triggering phreatic explosions.
Study on spatial evolution and mechanism of county economic differences in Hubei province from 2005 to 2020
Authors: Jiang Liang , Chen Jie , Zhang Chunyan , Tian Ye , Wu Guoxi , Luo Jing
Published: August 2022

The spatial distribution pattern of the economic development among counties is an important external representation of a balanced and sustainable regional development in China. With the rapid development of globalization and localization, spatial pattern of economic growth is increasingly obvious. The mechanisms of regional economic growth in China are also gradually gaining attention. However, there is still a lack of research at the province and county levels. As a result, based on the per capita GDP of each county in Hubei province from 2005 to 2020 as the research index, the spatial autocorrelation and the spatial variation function are used to analyze the spatial pattern evolution and the county economy mechanism in Hubei province. The results show that 1) there is a remarkable phenomenon of county-level economic spatial agglomeration in Hubei province. The urban area of Wuhan and its surrounding counties are high–high (HH-type) county agglomeration areas. The low–low (LL-type) counties are mainly distributed in the western parts of Hubei province and scattered in the northeastern and southern parts of Hubei province; 2) the county economy of Hubei province presents a spatial distribution pattern of “high in the east and low in the west.” The hot areas of the county economy are primarily located in the urban area of Wuhan and its surrounding areas. In the process of development, the hot spot areas tend to shift to Yichang, Jingmen, and Xiangyang. The cold spot areas are located on the edges of the western, northeastern, and southeastern areas of Hubei province; 3) the spatial continuity and self-organization of the county economic development are strengthened. The structural differentiation trend caused by spatial autocorrelation is also strengthened. The county economy is relatively balanced from the southeast to the northwest, and the spatial difference in economic development in other directions is increasing; and 4) the spatial evolution of county economic development in Hubei province is the result of the comprehensive effects of historical and cultural background, economic development, traffic location, and policy system, and the A-shaped point-axis structure is a reliable spatial structure for regional development in Hubei province.
Proactive stress interference mechanism and its application in the Mahu oil area, Junggar basin
Authors: Wang Shuo , Li Xiaoshan , Hao Lihua , Zhang Xiaogong , Zhang Lifeng , Song Junqiang , Liu Rongjun , Pan Hong , Gu Kaifang , Li Ting , Luo Guanxing , Wang Xin , Li Shihong
Published: August 2022

There exists a large horizontal principal stress difference (11–38 MPa) in the tight conglomerates in the Mahu oil area, China. It is difficult to form a complex fracture network via hydraulic fracturing under these conditions. To improve reservoir stimulation, the fracture formation mechanism of the complex conglomerate fracture networks was explored. Based on the geomechanics theory of fracture formation, the mechanism of the “stress wall” formed by fracturing in horizontal wells was analyzed in this paper. The inhibitory effect of the stress wall on the formation of tensile and shear fractures was studied. The reason for the decrease in the stress difference coefficient caused by fracturing fluid was analyzed through numerical simulation, which suggested that the complexity of a fracturing network is mainly controlled by the interference of externally applied stress and the reduction in the coefficient of internal stress difference. In this paper, innovative technologies were developed by proactively introducing stress interference in the application of the Ma131 small-well-spacing pilot area. The core technologies include optimization of the 3-D staggered small-well-spacing pattern, and synergetic optimization of multiple elements and zipper fracturing. The positive effects of proactive stress interference on improving fracturing volume, reserve utilization rate and recovery were discussed. Based on the concept of proactive stress interference, the “serial fracturing mode” of horizontal wells was proposed to reduce drilling and fracturing interference and improve the development effect.
Effects of Water, Sanitation, and Hygiene on the School Absenteeism of Basic Level Students in the Government School of Nepal
Authors: Sharma Mohan Kumar , Adhikari Ramesh
Published: August 2022

This study assesses the effect of improved water, sanitation, and hygiene (WASH) services on students’ absenteeism in government basic schools for 10–19 years old in Nepal. This study applied an ex post facto research design on two groups of students: with and without improved school WASH services. This study showed that students in schools with improved WASH services were more likely to be regular in attendance (80%) compared to those without (58%), p < 0.001. There was an association between students’ school absenteeism and student grades, p < 0.05. Moreover, the caste, religion, and grades of students were also associated with students’ school absenteeism, p < 0.001. The analysis further showed that students with improved WASH were more likely to be regular [crude odds ratios (COR) = 0.353; 95% confidence interval (CI); 0.256–0.487, p < 0.001] than those without. This trend was maintained across all demographics. It was significant even after the inclusion of all sociodemographic characteristics and increased rapidly [adjusted odds ratio (AOR) = 0.508; 95% CI; 0.334–0.773, p < 0.01]. The Brahmin/Chhetri-Terai and the Dalit students were more likely to be absent (COR = 0.315; 95% CI; 0.153–0.648, p < 0.01 and AOR = 0.274; 95% CI; 0.139–0.542, p < 0.001, respectively) than Brahmin/Chhetri-Hill, Janajati, and other castes. This study underscores the importance of WASH services in schools in reducing students’ school absenteeism. Access to WASH services is recommended with several awareness programs to reduce absenteeism and increase students’ school regularity.
Design and analysis of an efficient photovoltaic energy-powered electric vehicle charging station using perturb and observe MPPT algorithm
Authors: Awad Mohamed , Ibrahim Ahmed M. , Alaas Zuhair Muhammed , El-Shahat Adel , Omar Ahmed I.
Published: August 2022

According to the current directive, we must rely on green energy for sustainable mobility. One of the green transition’s goals is to use renewable energy to charge electric vehicles (EVs). Solar energy is a form of renewable energy sources, and it is classified as being clean, available, and renewable because it is based on sunshine. Nowadays, the world is turning to EVs which have lower running costs and cleaner environments. Lithium-ion batteries are commonly utilized to store energy in EVs. This article covers the design and analysis of a photovoltaic (PV) system to charge five models of EVs such as BMW i3 2019, Volkswagen e-Golf, Fiat 500e, Mercedes EQA 250, and Hyundai Kona Electric in a DC fast charging mode by using a buck converter to minimize the output voltage and without any addition of energy storage. By applying Perturb & Observe (P&O) Maximum Power Point Tracking (MPPT), the maximum power and efficiency from PVs are obtained. The charging time is calculated for each EV of the five models in the State of Charge (SOC) area at 20–80 percent. A MATLAB program is employed to simulate the EV models by calculating the efficiency of the MPPT controller, time of charging, and characteristic of voltage and current levels for each model of these EVs. All models are tested under the condition of irradiance level from 600 W/m2 to 1000 W/m2 and temperature between 20°C and 30°C. The results showed that the PV system is effective and economical as a stand-alone to charge EVs in a rapid charge mode.
A Conceptual Framework for Collaborative Development of Intelligent Construction and Building Industrialization
Authors: Guo Zhenxu , Li Lihong
Published: August 2022

Digitalization drives the arrival of the era of intelligent construction, which greatly affects the development of construction industrialization. Due to the collaborative barriers between intelligent construction and building industrialization (ICBI), it is difficult for construction enterprises to participate in the actual engineering projects in this work. Achieving the collaborative development of ICBI has become a way to transform and upgrade the construction industry, and it has attracted more and more attention. However, the existing literature lacks consensus on the collaborative development of ICBI affecting the construction industry. In order to promote the theoretical research on the collaborative development of ICBI, this paper proposes a “mi” shaped conceptual framework so that readers can understand the operation law of ICBI collaboration and promote its healthy development. Therefore, based on the synergy theory, this paper extracts the contents of stakeholders’ collaboration, industrial collaboration, structural collaboration, construction technology upgrading, factor endowment upgrading, innovation service upgrading, and construction process upgrading. These seven items are summarized into four dimensions: dual drive, resource supply, collaborative operation, and trust guarantee, which indicate the direction of ICBI collaborative development. The research results will help to realize the industrialization of new buildings and promote the sustainable development of the construction industry.
The resilient economic impact of CPEC and future of MNCs: Evidence from Pakistan
Authors: Anwar Syed Umair , Wuyi Zhang , Ali Shah Syed Zahid , Ullah Qudrat , Amir Syed Muhammad , Syed Ammara
Published: August 2022

This article examines the developmental collaboration under CPEC to see if the stated scenario of developed-country progressive cooperation with developing countries holds true on the ground. The economic development of countries is determined by their economic relationships with other countries including megaprojects like CPEC which interlink countries geographically, socially, and economically. The present study has adopted a mixed method technique and the data for this study was gathered using questionnaires and one-on-one interviews with respondents. Furthermore, Pearson correlation, reliability, and KMO were used for analysis. The findings revealed that except for GDP growth and poverty alleviation, all dimensions of resilient economic development such as infrastructure development, investment, economic growth, employment, transportation and knowledge transfer initiatives have positive relationship with CPEC development. Our findings reveal that the GDP and welfare of both Pakistan and China will improve by a maximum of 0.3 percent as a result of transportation innovation. Moreover, the results of the study positively validate the hypothesis. These findings provide policymakers with guidelines for establishing effective policies to support the mega-plan CPEC which will boost global economic growth in the region.
Comparative study on the calculation methods of ecological base flow in a mountainous river
Authors: Gao Cheng , Hao Manqiu , Song Lianghong , Rong Wang , Shao Shuaibing , Huang Ying , Guo Yufa , Liu Xueyao
Published: August 2022

As the social economy is further developed, there have been increasingly severe ecological and environmental issues. For instance, rivers located in mountainous areas are often encountered with the issue of zero flow. The ecological base flow was put forward to ensure the continuous flow of rivers, to realize the minimum ecological function, and to meet the most fundamental requirements of sustaining the river ecosystem. Wulong River Basin in Yantai City of China was taken as example here. Based on the runoff data collected in the Tuanwang Hydrological Station from 1960 to 2016, the measured runoff data was restored to natural runoff sequence by adopting the item-by-item investigation method. The different ecological base flows of Wulong River were calculated by adopting the Tennant Method, the Qp90 Method, the Texas Method, the Base Flow Ratio Method, the Tessman Method, and Wetted Perimeter Method. The research findings have shown that the Base Flow Ratio Method proves to be the optimal approach for calculating the ecological base flow of Wulong River. Based on the analysis of the assurance degree of ecological base flow, the period from April to June is the period with the lowest assurance degree of ecological base flow.
Causality analytics among key factors for green economy practices: Implications for sustainable development goals
Authors: Karuppiah Koppiahraj , Sankaranarayanan Bathrinath , Ali Syed Mithun , AlArjani Ali , Mohamed Abdullah
Published: August 2022

The green economy (GE) concept is believed to have the ability to turn natural resources into wealth in a sustainable manner. As a result, the GE concept is viewed as a magic key to the attainment of sustainable development goals (SDGs). This study aims to identify and evaluate the critical factors in GE practices. Based on literature review and industrial interaction, five dimensions of critical factors, namely, economic and market, technical and R&D, policy and regulation, networks and social capital, and public perceptions, with a total of 20 critical factors were identified and evaluated. The Fermatean fuzzy system (FFS)-based decision-making trial and evaluation laboratory (DEMATEL) technique has been used to evaluate the causality among the critical factors. Results reveal that win-win anticipations, the commitment of key stakeholders, the domestic market structure, cost of alternatives, and financial assistance are the top five critical factors in GE practices. Three critical factors are classified under the cause category, and the remaining 17 critical factors come under the effect category. This study contributes to the literature on GE by revealing the causal interrelationship among the critical factors. This will guide industrial management to take appropriate actions in the implementation of GE practices.
Soil Seed Bank Characteristics of Nitraria tangutorum Nebkhas in a Desert–Oasis Ecotone
Authors: Li Min , Xiao Huijie , Xin Zhiming , Li Xing , Li Junran , Miri Abbas , Cao Qiqi
Published: August 2022

Understanding soil seed banks (SSBs) of Nitrariatangutorum nebkhas is critical for vegetation restoration and ecological management in desert–oasis ecotones. In this study, we conducted a survey in the field and a seed germination experiment in the laboratory to investigate the characteristics of SSB in different sizes and parts of nebkhas and to examine their relationship with aboveground vegetation. The results showed that there were 17 species of plants in six families in the SSB of Nitraria tangutorum nebkhas in a desert–oasis ecotone, dominated by Chenopodiaceae. The life forms were mainly annual herbs, accounting for 58.82%–71.43%. The number of species in the SSB were in the order of large nebkha > medium nebkha > small nebkha, and there was no significant difference in SSB density. However, SSB densities in different parts of each nebkha were in the order bottom > middle > top. The densities were significantly different and showed a decreasing trend as soil depth increased. Most of the plant seeds were distributed in the 0–5 cm soil layer. The similarity coefficient between the SSB of each nebkha and the aboveground vegetation was high. From the bottom to the top, the number of common species and similarity coefficient between the aboveground vegetation and the SSB decreased, and the number of species in the SSB was greater than that of the aboveground vegetation. Vegetation plays a decisive role in the initial development of a nebkha. When the nebkha develops to a certain extent, it reacts to the vegetation, thereby intensifying the differences in the composition of vegetation species in different parts of the nebkha and further affecting the SSB.
Water and nutrient recovery from stored urine by forward osmosis with an ammonium bicarbonate draw solution
Authors: Pocock J. , Muzhingi A. , Mercer E. , Velkushnova K. , Septien S. , Buckley C. A.
Published: August 2022

Forward osmosis with an ammonium bicarbonate draw solution was investigated as a low energy non-sewered sanitation solution, to recover nutrients and water from source separated urine. Stored urine collected from Urine Diversion Dry Toilets in the eThekwini Municipality (Durban), South Africa was used as the feed solution. Water recoveries of up to 45.9% with water fluxes up to 6.0 L m−2 h−1 were achieved using undiluted stored urine over an 8-h operating period with a 2.5 M draw solution. Rejections of up to 95% for phosphates, 85% for nitrogen and chlorides, and 75% for potassium and sodium were achieved. Low fouling of the membrane was observed after multiple runs and cleaning the membrane by circulation of deionized water or by osmotic backwash was sufficient to recover >95% of the original water flux. Little irreversible fouling was detected, assumed to be caused by carbonate calcium scaling from SEM-EDX analysis. This study suggests that forward osmosis with an ammonium bicarbonate solution could be integrated as a closed loop nutrient recycling technology for source separated urine with the prospect of clean water and draw solution recovery that could use waste heat from, for example, fecal sludge combustion.
Riverine macroplastic gradient along watercourses: A global overview
Authors: Gallitelli Luca , Scalici Massimiliano
Published: August 2022

Nowadays, plastics represent growing concern in aquatic habitats, harming more and more both ecosystem and human health. Since rivers are the main carriers of plastics to the sea, some studies were conducted on the transport of overall small plastics, but observations on big plastics (i.e., macroplastics) in rivers are poorly reported in the literature. Although most studies focused on plastic transport in the lower part of the rivers, research on the upper and middle zones are completely lacking. In this regard, this could influence the transport of plastics to the sea, with insights into the source and origin of macroplastics. Here, we aimed at overviewing macroplastic distribution along rivers, emphasizing on the global riverine plastic hotspot areas. First, 1) we analyzed and discussed the bibliometric analysis on macroplastics in rivers providing a framework for plastic pollution management. second, 2) we provided geographical insights into macroplastics by mapping global riverine plastic hotspot areas. Then, 3) we analyzed the main factors affecting macroplastic distribution and accumulation in rivers. Furthermore, 4) we assessed crucial gaps in riverine macroplastic accumulation, highlighting the importance of a plastic gradient in the horizontal transport along the entire river course (i.e., three river zones). Then, 5) we highlighted the lack of standardization regarding macroplastic size, concentration, and polymers that does not allow valuable comparisons among studies. At the end, 6) we concluded by providing future perspectives and conclusions on macroplastic distribution and accumulation in rivers worldwide. Our results might provide new insights into a comprehensive framework of macroplastic distribution along global rivers, suggesting the river rod approach as a way for future monitoring as all the complete course of a river plays a pivotal role in accumulating macroplastics.
The production and secretion of tRNA-derived RNA fragments in the corn smut fungus Ustilago maydis
Authors: Yoshimoto Rei , Ishida Fumiko , Yamaguchi Miyuki , Tanaka Shigeyuki
Published: August 2022

The biogenesis of small non-coding RNAs is a molecular event that contributes to cellular functions. The basidiomycete fungus Ustilago maydis is a biotrophic pathogen parasitizing maize. A hallmark of its genome structure is an absence of RNAi machinery including Dicer and Argonaute proteins, which are responsible for the production of small RNAs in other organisms. However, it remains unclear whether U. maydis produces small RNAs during fungal growth. Here we found that U. maydis cells accumulate approximately 20-30 nucleotides of small RNA fragments during growth in the axenic culture condition. The RNA-seq analysis of these fragments identified that these small RNAs are originated from tRNAs and 5.8S ribosomal RNA. Interestingly, majority of their sequences are generated from tRNAs responsible for asparagine, glutamine and glycine, suggesting a bias of origin. The cleavage of tRNAs mainly occurs at the position near anticodon-stem-loop. We generated the deletion mutants of two genes nuc1 and nuc2 encoding RNase T2, which is a candidate enzyme that cleaves tRNAs. The deletion mutants of two genes largely fail to accumulate tRNA-derived RNA fragments. Nuc1 and tRNA are co-localized at the tip of budding cells and tRNA fragment could be detected in culture supernatant. Our results suggest that specific tRNAs would be cleaved during secretory processes and tRNA fragments might have extracellular functions.
Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses
Authors: Singh Rajesh Kumar , Singh Charul , Ambika  , Chandana B. S. , Mahto Rohit K. , Patial Ranjana , Gupta Astha , Gahlaut Vijay , Gayacharan  , Hamwieh Aladdin , Upadhyaya H. D. , Kumar Rajendra
Published: August 2022

Legume crops provide significant nutrition to humans as a source of protein, omega-3 fatty acids as well as specific macro and micronutrients. Additionally, legumes improve the cropping environment by replenishing the soil nitrogen content. Chickpeas are the second most significant staple legume food crop worldwide behind dry bean which contains 17%–24% protein, 41%–51% carbohydrate, and other important essential minerals, vitamins, dietary fiber, folate, β-carotene, anti-oxidants, micronutrients (phosphorus, calcium, magnesium, iron, and zinc) as well as linoleic and oleic unsaturated fatty acids. Despite these advantages, legumes are far behind cereals in terms of genetic improvement mainly due to far less effort, the bottlenecks of the narrow genetic base, and several biotic and abiotic factors in the scenario of changing climatic conditions. Measures are now called for beyond conventional breeding practices to strategically broadening of narrow genetic base utilizing chickpea wild relatives and improvement of cultivars through advanced breeding approaches with a focus on high yield productivity, biotic and abiotic stresses including climate resilience, and enhanced nutritional values. Desirable donors having such multiple traits have been identified using core and mini core collections from the cultivated gene pool and wild relatives of Chickpea. Several methods have been developed to address cross-species fertilization obstacles and to aid in inter-specific hybridization and introgression of the target gene sequences from wild Cicer species. Additionally, recent advances in “Omics” sciences along with high-throughput and precise phenotyping tools have made it easier to identify genes that regulate traits of interest. Next-generation sequencing technologies, whole-genome sequencing, transcriptomics, and differential genes expression profiling along with a plethora of novel techniques like single nucleotide polymorphism exploiting high-density genotyping by sequencing assays, simple sequence repeat markers, diversity array technology platform, and whole-genome re-sequencing technique led to the identification and development of QTLs and high-density trait mapping of the global chickpea germplasm. These altogether have helped in broadening the narrow genetic base of chickpeas.
Comparative de novo transcriptome analysis of flower and root of Oliveria decumbens Vent. to identify putative genes in terpenes biosynthesis pathway
Authors: Khodavirdipour Amir , Safaralizadeh Reza , Haghi Mehdi , Hosseinpourfeizi Mohammad Ali
Published: August 2022

The Oliveria decumbens Vent. is a wild, rare, annual medicinal plant and endemic plant of Iran that has metabolites (mostly terpenes) which make it a precious plant in Persian Traditional Medicine and also a potential chemotherapeutic agent. The lack of genetic resources has slowed the discovery of genes involved in the terpenes biosynthesis pathway. It is a wild relative of Daucus carota. In this research, we performed the transcriptomic differences between two samples, flower and root of Oliveria decumbens, and also analyze the expression value of the genes involved in terpenoid biosynthesis by RNA-seq and its essential oil’s phytochemicals analyzed by GC/MS. In total, 136,031,188 reads from two samples of flower and root have been produced. The result shows that the MEP pathway is mostly active in the flower and the MVA in the root. Three genes of GPP, FPPS, and GGPP that are the precursors in the synthesis of mono, di, and triterpenes are upregulated in root and 23 key genes were identified that are involved in the biosynthesis of terpenes. Three genes had the highest upregulation in the root including, and on the other hand, another three genes had the expression only in the flower. Meanwhile, 191 and 185 upregulated genes in the flower and root of the plant, respectively, were selected for the gene ontology analysis and reconstruction of co-expression networks. The current research is the first of its kind on Oliveria decumbens transcriptome and discussed 67 genes that have been deposited into the NCBI database. Collectively, the information obtained in this study unveils the new insights into characterizing the genetic blueprint of Oliveria decumbens Vent. which paved the way for medical/plant biotechnology and the pharmaceutical industry in the future.
A pan-cancer analysis reveals the genetic alterations and immunotherapy of Piezo2 in human cancer
Authors: Liu Xin , Jia Yangpu , Wang Zhihui , Zhang Zhaoxiong , Fu Weihua
Published: August 2022

Background: Piezo2 is a transmembrane-spanning ion channel protein implicated in multiple physiological processes, including cell proliferation and angiogenesis in many cell types. However, Piezo2 was recognized as representing a double-edged sword in terms of tumor growth. The prognostic and immunotherapeutic roles of Piezo2 in pan-cancer have not been reported.

Methods: In this study, several databases available including the UCSC Xena database, HPA, TIDE, GSEA, and cBioportal were used to investigate the expression, alterations, associations with immune indicators, and prognostic roles of Piezo2 across pan-cancer. R software and Perl scripts were used to process the raw data acquired from the UCSC Xena database.

Results: Based on processed data, our results suggested that Piezo2 expression levels were tissue-dependent in different tumor tissues. Meanwhile, the survival analysis reflected that patients suffering from KIRC, LUAD, and USC with high Piezo2 expression had good OS, while those suffering from KIRP and SARC with high Piezo2 expression had poor OS. In addition, our results showed that Piezo2 expression was associated with the infiltration of CD4+ T memory cells, mast cells, and dendritic cells. These results suggested that Piezo2 may involve tumor progression by influencing immune infiltration or regulating immune cell function. Further analysis indicated that Piezo2 could influence TME by regulating T-cell dysfunction. We also found that gene mutation was the most common genetic alteration of Piezo2. The GSEA analysis revealed that Piezo2 was associated with calcium ion transport, the activation of the immune response, antigen processing and presentation pathways.

Conclusion: Our study showed the expression and prognostic features of Piezo2 and highlighted its associations with genetic alterations and immune signatures in pan-cancer. Moreover, we provided several novel insights for further research on the therapeutic potential of Piezo2.
CaMKII is a modulator in neurodegenerative diseases and mediates the effect of androgen on synaptic protein PSD95
Authors: Mi Shixiong , Chen Huan , Lin Peijing , Kang Peiyuan , Qiao Dan , Zhang Bohan , Wang Zhao , Zhang Jingbao , Hu Xiangting , Wang Chang , Cui Huixian , Li Sha
Published: August 2022

Androgens rapidly regulate synaptic plasticity in hippocampal neurones, but the underlying mechanisms remain unclear. In this study, we carried out a comprehensive bioinformatics analysis of functional similarities between androgen receptor (AR) and the synaptic protein postsynaptic density 95 (PSD95) to evaluate the effect. Using different measurements and thresholds, we obtained consistent results illustrating that the two proteins were significantly involved in similar pathways. We further identified CaMKII plays a critical role in mediating the rapid effect of androgen and promoting the expression of PSD95. We used mouse hippocampal neurone HT22 cells as a cell model to investigate the effect of testosterone (T) on intracellular Ca2+ levels and the mechanism. Calcium imaging experiments showed that intracellular Ca2+ increased to a peak due to calcium influx in the extracellular fluid through L-type and N-type voltage-gated calcium channels when HT22 cells were treated with 100 nM T for 20 min. Subsequently, we investigated whether the Ca2+/CaMKII signaling pathway mediates the rapid effect of T, promoting the expression of the synaptic protein PSD95. Immunofluorescence cytochemical staining and western blotting results showed that T promoted CaMKII phosphorylation by rapidly increasing extracellular Ca2+ influx, thus increasing PSD95 expression. This study demonstrated that CaMKII acts as a mediator assisting androgen which regulates the synaptic protein PSD95Also, it provides evidence for the neuroprotective mechanisms of androgens in synaptic plasticity and reveals the gated and pharmacological mechanisms of the voltage-gated Ca2+ channel family for androgen replacement therapy.
Assessing the potential vulnerability of sedimentary carbon stores to bottom trawling disturbance within the UK EEZ
Authors: Black Kirsty E. , Smeaton Craig , Turrell William R. , Austin William E. N.
Published: August 2022

It is estimated that within the UK exclusive economic zone (UK EEZ), 524 Mt of organic carbon (OC) is stored within seabed sediment. However, the stability and potential vulnerability of OC in these sediments under anthropogenic stressors, such as bottom trawling activity, remains poorly quantified. To improve our understanding of the areas where sedimentary OC is likely to be at greatest risk from trawling events, we have developed a carbon vulnerability ranking (CVR) to identify areas of the seabed where preventative protection may be most beneficial to help maintain current OC stocks while further research continues to shed light on the fate of OC after trawling (e.g., remineralization, transport, and consumption). Predictive maps of currently available fishing intensity, OC and sediment distribution, and sediment OC lability have been generated within ArcGIS using fuzzy set theory. Our results show that the west coast of Scotland represents one of the key areas where sedimentary OC is potentially at greatest risk from bottom trawling activity. This is due mainly to the high reactivity of these OC rich sediments combined with the pressures of repetitive trawling activity within inshore waters. Our research shows that these OC hotspots are potentially at risk of disturbance from bottom trawling activity and should be prioritized for the consideration of future safeguarding (management) measures to ensure emissions are minimized and to provide greater protection of this natural carbon capital resource.
The role of aquaria in the advancement of elasmobranch reproductive biology
Authors: Feldheim Kevin A. , Wyffels Jennifer T. , Lyons Kady
Published: August 2022

Public aquaria showcase aquatic life while raising awareness and educating the public on biodiversity and the need for conservation. Recently, aquaria have followed in the footsteps of zoos by taking more directed approaches in species conservation as well as leveraging animals in their collection to fill biological knowledge gaps through research. Similar to zoos, aquaria are able to house animals that are not feasible to care for in traditional academic settings, allowing important life history information to be gained. In particular, our understanding of reproductive aspects of large migratory fishes such as elasmobranchs (sharks, skates and rays) have benefited from information gleaned in aquarium settings that would otherwise be difficult or impossible to study in the natural environment. For example, the ability of elasmobranchs to reproduce parthenogenetically was discovered through observation of sharks maintained in public aquaria. Since its discovery, parthenogenesis has been observed for many ex situ shark and ray species. Aquaria have made other important contributions to characterizing elasmobranch reproductive biology such as understanding reproductive cycling and trialing assisted reproductive techniques, among others. Here, we review the role aquaria have played in the field of elasmobranch reproductive biology and discuss how zoos and aquariums can continue to contribute to this field in the future.
Coats disease in female population: A comparison of clinical presentation and outcomes
Authors: Piquin Gwendoline , Chapron Thibaut , Abdelmassih Youssef , Martin Gilles , Edelson Catherine , Caputo Georges , Metge Florence
Published: August 2022

Purpose

To compare clinical characteristics at presentation and outcomes of Coats disease between females and males.

Methods

In this retrospective, consecutive case series we included all children diagnosed with Coats disease in a single tertiary referral center. Initial clinical presentation, treatment and outcomes were collected.

Results

A total of 158 children were included, of whom 29 (18.3%) were females and 11 (6.9%) had bilateral involvement. Age at diagnosis and disease stage were similar between females and males. Females had more bilateral involvement (p < 0.001) and tended to have a worse visual acuity at diagnosis (p = 0.05). At last follow-up, visual acuity and anatomical outcome after treatment were similar between genders.

Conclusion

Female patients with Coats disease had more bilateral involvement and tended to have worse visual acuity at presentation. Clinical presentation and outcomes seemed to be similar between genders.
Sex differences in clinical trials of ALRV5XR treatment of androgenetic alopecia and telogen effluvium
Authors: Feldman Peter R. , Feldman Oriel J. , Guevara-Aguirre Jaime , Fiebig Klaus M.
Published: August 2022

Introduction

ALRV5XR treatment of androgenetic alopecia (AGA) and telogen effluvium (TE) has early evidence of regenerating a normal scalp hair phenotype in both sexes.

Design

We performed two 24-week double-blinded placebo-controlled comparison trials, one in each sex, on the ALRV5XR treatment effect on hair regeneration, in AGA and TE, in 92 AGA subjects (24 also had TE). Forty-six women (age 24–64 years) and 46 men (age 22–63 years) were randomized 1:1 to either ALRV5XR or placebo regimens (one b.i.d. oral capsule and daily administration of shampoo, conditioner, and follicle serum).

Evaluation

Primary outcomes: Absolute and relative changes in terminal hair (TH) density. Secondary outcomes: Response rate, changes in vellus hair (VH) density, TH/VH ratio, hair diameter, growth, and shedding rate.

Results

Forty-one women (20 ALRV5XR, 21 placebo) and 36 men (17 ALRV5XR, 19 placebo) completed the trials. TH outcome was evaluable for 18 and 21 women and 11 and 11 men (ALRV5XR, placebo, respectively). Efficacy in women: 30.1 THs/cm2 (p = 0.0002) and 19.7% (p = 0.0016). Efficacy in men: 21.0 THs/cm2 (p = 0.0014) and 16.4% (p = 0.0012). 66.7% of women and 100% of men responded to ALRV5XR. TH/VH ratio for men increased 33.0% (p = 0.0033). Growth rate in women increased by 30.7 μm/24 h (p < 0.0001) and 10.0% (p < 0.0001). There were no adverse events reported.

Conclusion and relevance

ALRV5XR induced significant regrowth of TH. Accelerating regrowth by reactivation of dormant telogen follicles were the dominant effects in women. Thickening of miniaturized hair and regrowth of dormant telogen follicles contributed equally to the increased TH seen in men (see Graphical Abstract).
Prognostic value of National Early Warning Score and Modified Early Warning Score on intensive care unit readmission and mortality: A prospective observational study
Authors: Mahmoodpoor Ata , Sanaie Sarvin , Saghaleini Seied Hadi , Ostadi Zohreh , Hosseini Mohammad-Salar , Sheshgelani Naeeme , Vahedian-Azimi Amir , Samim Abbas , Rahimi-Bashar Farshid
Published: August 2022

Background

Modified Early Warning Score (MEWS) and National Early Warning Score (NEWS) are widely used in predicting the mortality and intensive care unit (ICU) admission of critically ill patients. This study was conducted to evaluate and compare the prognostic value of NEWS and MEWS for predicting ICU readmission, mortality, and related outcomes in critically ill patients at the time of ICU discharge.

Methods

This multicenter, prospective, observational study was conducted over a year, from April 2019 to March 2020, in the general ICUs of two university-affiliated hospitals in Northwest Iran. MEWS and NEWS were compared based on the patients’ outcomes (including mortality, ICU readmission, time to readmission, discharge type, mechanical ventilation (MV), MV duration, and multiple organ failure after readmission) using the univariable and multivariable binary logistic regression. The receiver operating characteristic (ROC) curve was used to determine the outcome predictability of MEWS and NEWS.

Results

A total of 410 ICU patients were enrolled in this study. According to multivariable logistic regression analysis, both MEWS and NEWS were predictors of ICU readmission, time to readmission, MV status after readmission, MV duration, and multiple organ failure after readmission. The area under the ROC curve (AUC) for predicting mortality was 0.91 (95% CI = 0.88–0.94, P < 0.0001) for the NEWS and 0.88 (95% CI = 0.84–0.91, P < 0.0001) for the MEWS. There was no significant difference between the AUC of the NEWS and the MEWS for predicting mortality (P = 0.082). However, for ICU readmission (0.84 vs. 0.71), time to readmission (0.82 vs. 0.67), MV after readmission (0.83 vs. 0.72), MV duration (0.81 vs. 0.67), and multiple organ failure (0.833 vs. 0.710), the AUCs of MEWS were significantly greater (P < 0.001).

Conclusion

National Early Warning Score and MEWS values of >4 demonstrated high sensitivity and specificity in identifying the risk of mortality for the patients’ discharge from ICU. However, we found that the MEWS showed superiority over the NEWS score in predicting other outcomes. Eventually, MEWS could be considered an efficient prediction score for morbidity and mortality of critically ill patients.
Causality and correlation analysis for deciphering the microbial interactions in activated sludge
Authors: Cai Weiwei , Han Xiangyu , Sangeetha Thangavel , Yao Hong
Published: August 2022

Time series data has been considered to be a massive information provider for comprehending more about microbial dynamics and interaction, leading to a causality inference in a complex microbial community. Granger causality and correlation analysis have been investigated and applied for the construction of a microbial causal correlation network (MCCN) and efficient prediction of the ecological interaction within activated sludge, which thereby exhibited ecological interactions at the OTU-level. Application of MCCN to a time series of activated sludge data revealed that the hub species OTU56, classified as the one belonging to the genus Nitrospira, was responsible for nitrification in activated sludge and interaction with Proteobacteria and Bacteroidetes in the form of amensal and commensal relationships, respectively. The phylogenetic tree suggested a mutualistic relationship between Nitrospira and denitrifiers. Zoogloea displayed the highest ncf value within the classified OTUs of the MCCN, indicating that it could be a foundation for activated sludge through the formation of characteristic cell aggregate matrices where other organisms embed during floc formation. Inclusively, the research outcomes of this study have provided a deep insight into the ecological interactions within the communities of activated sludge.
Interactive removal of bacterial and viral particles during transport through low-cost filtering materials
Authors: Chen Xijuan , Yang Liqiong , Guo Junjie , Xu Shuang , Di Junzhen , Zhuang Jie
Published: August 2022

Pathogen filtration is critically important for water sanitation. However, it is a big challenge to balance removal efficiency and filtering material cost. In this study, we quantified the removal processes of a bacterial strain Escherichia coli 652T7 and a model bacteriophage MS2 (ATCC 15597-B1) during their transport through columns containing iron filings (IF), calcined magnesite (CM), natural ore limestone (OL) or corn stalk biochar (BC) under saturated flow conditions. Experimental results showed that 99.98, 79.55, 63.79, and 62.59% of injected E. coli 652T7 and 98.78, 92.26, 68.79, and 69.82% of injected MS2 were removed by IF, CM, OL, and BC, respectively. The differences in removal percentage were attributed to the disparities of the microorganisms and filtering materials in surface function groups, surface charges, and surface morphology. Transport modeling with advection-dispersion equation (ADE) and interaction energy calculation with extended Derjaguin, Landau, Verwey, and Overbeek (XDLVO) model indicated that E. coli 652T7 and MS2 were mostly removed via irreversible attachment. In IF columns, E. coli 652T7 promoted the transport of MS2 but not vice versa. In CM columns, MS2 facilitated the transport of E. coli 652T7 and vice versa at a less extent. Such changes were a combined result of attachment site competition, steric effect, and mechanical straining. We found that the sum of the removal percentages of the two microorganisms in their respective transport experiments were similar to those calculated from their co-transport experiments. This result suggests that the removals were mainly limited by the attachment sites in the filtering materials.
Multi-epitope chimeric vaccine designing and novel drug targets prioritization against multi-drug resistant Staphylococcus pseudintermedius
Authors: Jaan Samavia , Shah Mohibullah , Ullah Najeeb , Amjad Adnan , Javed Muhammad Sameem , Nishan Umar , Mustafa Ghazala , Nawaz Haq , Ahmed Sarfraz , Ojha Suvash Chandra
Published: August 2022

Biofilm synthesizing multi-drug resistantStaphylococcus pseudintermediusbacteria has been recognized as the human infectious agent. It has been detected in the diseases of skin, ear, and postoperative infections. Its infections are becoming a major health problem due to its multi-drug resistance capabilities. However, no commercial vaccine for the treatment of its infections is currently available in the market. Here we employed the subtractive proteomics and reverse vaccinology approach to determine the potential novel drug and vaccine targets againstS. pseudintermediusinfections in humans. After screening the core-proteome of the 39 complete genomes ofS. pseudintermedius, 2 metabolic pathways dependent and 34 independent proteins were determined as novel potential drug targets. Two proteins were found and used as potential candidates for designing the chimeric vaccine constructs. Depending on the properties such as antigenicity, toxicity and solubility, multi-epitope based vaccines constructs were designed. For immunogenicity enhancement, different specific sequences like linkers, PADRE sequences and molecular adjuvants were added. Molecular docking and molecular dynamic simulation analyses were performed to evaluate the prioritized vaccine construct’s interactions with human immune cells HLA and TLR4. Finally, the cloning and expression ability of the vaccine construct was determined in the bacterial cloning system and human body immune response was predicted through immune simulation analysis. In conclusion, this study proposed the potential drug and vaccine targets and also designed a chimera vaccine to be tested and validated against infectiousS. pseudintermediusspecies.