bluuebunny/crossref_metadata_2025 huggingface dataset:
Vo2 máximo obtido com e sem teste ergométrico
Authors: Ribeiro Cléo Pereira , Daronco Luciane Sanchotene Etchepare , Jacobi Luciane Flores , Silva Luciano Panosso da
Published: March 2020

Objetivo: verificar a concordância e a correlação entre duas metodologias distintas, utilizadas na obtenção do VO2  máx, as propostas por Foster et al. (T1) e Jackson et al., modelo 2, (T2). Metodologia: Os dados para análise, pertencentes a uma clínica privada em nosso município, foram obtidos mediante a assinatura dos Termos de Confidencialidade e Autorização Institucional. De acordo com os critérios de inclusão, 106 sujeitos (29 homens; 77 mulheres) formaram a amostra. O teste de Shapiro-Wilk, a análise descritiva, a análise de Bland e Altman e o coeficiente de correlação de Pearson, compuseram a análise estatística (nível de significância de 5%). Resultados: apesar das semelhanças, entre as médias obtidas, as metodologias demonstraram concordância somente no grupo masculino. Conclusão: em função dos resultados obtidos, e da enorme praticidade, sugere-se o método T2 para fins de prescrição do exercício, mas, o método T1 torna-se indispensável para avaliar a integridade do sistema cardiorrespiratório.
Fisioterapia na disfunção temporomandibular
Authors: Vasconcelos Roberta Simões Nogueira , Marques Lidia Audrey Rocha Valadas , Kuehner Maria Cymara Pessoa , Barroso Karoline Sampaio Nunes , Dias Camila Costa , Carmo Filho José Ronildo Lins do , Fiallos Ana Cristina De Mello , Fernandes Mariana Lima
Published: March 2020

OBJETIVO: Avaliar os resultados do tratamento fisioterapêutico em pacientes com disfunção temporomandibular (DTM). MATERIAS E MÉTODOS: Foram coletados dados dos prontuários de uma Clínica Universitária de Fisioterapia na cidade de Fortaleza. Os itens avaliados nos prontuários foram idade, gênero, ocupação, queixa principal, doenças associadas e tratamentos prévios. RESULTADOS: A predominância dos pacientes foi do gênero feminino (76,5%) e estudantes universitários da instituição. Entre as principais queixas encontram-se dor na ATM e músculos mastigatórios, ruídos da ATM, limitação para abertura bucal e cefaléia. Em associação à DTM, foram encontradas principalmente alterações posturais e fibromialgia. Correções ortodônticas e uso de placas foram os tratamentos odontológicos mais realizados pelos pacientes. Mais da metade dos pacientes obtiveram melhoras significativas, como: redução da dor muscular e na ATM, aumento da amplitude de movimento, ajustes posturais, diminuição dos estalidos e da cefaleia. CONCLUSÃO: A intervenção fisioterapêutica foi decisiva para o controle dos sintomas e prevenção de agravos, proporcionando uma melhora da funcionalidade.
Incubadora educacional: agregando tecnologias à prática do cuidado no ambiente de trabalho hospitalar
Authors: Pereira Liliane Alves , Vidal Danielle Adriane Silveira , Passos Cintia Marchesan , Bueno Maria Emília
Published: March 2020

Objetivou-se conhecer as contribuições da Incubadora Educacional na perspectiva de novos colaboradores de enfermagem que participaram do processo de capacitação no ambiente de trabalho hospitalar. Métodos: pesquisa qualitativa, com 35 novos colaboradores sendo técnicos de enfermagem e enfermeiros, no período de julho de 2015 a julho de 2016, em um hospital de ensino da região sul do Brasil. A leitura dos dados foi realizada na perspectiva de Bardin. Resultados: Os colaboradores consideraram a incubadora educacional como adequada quanto a duração, conteúdo e método de ensino. Destacaram-se os núcleos temáticos: “A incubadora educacional como um processo de reciclagem” e “A integração entre teoria e prática no processo de aprendizagem”. Conclusão: A Incubadora Educacional contribuiu de forma positiva no processo de capacitação dos novos colaboradores. Possibilitou um espaço de argumentações e reflexões sobre as práticas cotidianas da instituição envolvida e a participação ativa dos profissionais na busca por mudanças positivas na assistência.
Associação entre aptidão cardiorrespiratória e hábitos sedentários em escolares do Sul do Brasil
Authors: Senha Andresa Fernanda , Schneiders Letícia De Borba , Borfe Letícia , Silveira João Francisco De Castro , Paiva Dulciane Nunes , Reuter Cézane Priscila
Published: March 2020

Este estudo transversal teve como objetivo verificar associação entre aptidão cardiorrespiratória (APCR) e hábitos sedentários em escolares de Santa Cruz do Sul – RS. A amostra foi constituída por 1.407 escolares, de 10 a 16 anos, de ambos os sexos, escolares da rede pública. Foi utilizado para avaliar os hábitos sedentários um questionário adaptado. Os níveis de APCR foram avaliados por meio do teste de corrida/caminhada de seis minutos, preconizado pelo Projeto Esporte Brasil. A regressão de Poisson foi aplicada para testar a associação entre a variável desfecho (APCR) com as variáveis independentes (hábitos sedentários). Os dados foram expressos em razão de prevalência (RP) e intervalo de confiança (IC) para 95%. Os adolescentes apresentaram prevalência elevada de baixos níveis de APCR (60,1%), deslocamento sedentário para a escola (66,5%) e alta frequência de tempo de tela igual ou superior de duas horas diárias (61,1%). Meninas que não praticam atividade física apresentam maior prevalência de baixos níveis de APCR (RP: 1,25; IC: 1,03-1,53; p=0,026). Pode-se concluir que baixos níveis de APCR estão associados a hábitos sedentários em escolares, o que pode resultar em complicações futuras em sua qualidade de vida.
Insatisfação com a imagem corporal em professores de Educação Física atuantes na Educação Infantil
Authors: Claumann Gaia Salvador , Pinto André De Araújo , Costa Andreina Medeiros , Bim Mateus Augusto , Pelegrini Andreia
Published: March 2020

Introdução: as condições de trabalho atuais podem ter inúmeras repercussões na saúde física e mental, destacando-se a imagem corporal de professores. Objetivo: analisar a insatisfação com a imagem corporal em professores de Educação Física atuantes na Educação Infantil. Método: participaram 38 professores (30 mulheres) da rede municipal de ensino de Florianópolis/SC, com média de idade de 35,7(9,0) anos. Foram coletadas informações demográficas, laborais, sobre imagem corporal (escala de silhuetas) e medidas antropométricas (massa corporal e estatura autorreferidas). Resultados: a insatisfação com a imagem corporal foi verificada em 68,4% dos professores. Aqueles que atuavam há dez anos ou mais no magistério (p= 0,040) e que trabalhavam até 40 horas por semana (p= 0,005) foram os mais insatisfeitos com a imagem corporal. Quanto ao status do peso, independentemente de apresentarem peso normal ou sobrepeso (p= 0,027), os professores estavam insatisfeitos. Conclusão: a maioria dos professores de Educação Física estava insatisfeita com sua imagem corporal, sendo que os mais insatisfeitos foram aqueles que atuavam na profissão há mais tempo, com maior carga horária semanal de trabalho e, independentemente, do status de peso.
TRANSPARÊNCIA ATIVA: UMA AVALIAÇÃO DOS MUNICÍPIOS DO ESTADO DE MATO GROSSO DO SUL
Authors: Chiariello Caio Luis , Paulucio Felipe Ferreira , Brito Ricardo França de , Assumpção Gisele de Souza , Verão Morrison Francisco Reis , Morais João Batista de
Published: March 2020

Com o advento da accountability, termos como transparência e governo eletrônico (e-government), passaram a ser mais usados, e a sociedade brasileira pôde experimentar uma nova era de acesso à informação, regida por normas e leis. Com o intuito de avaliar a transparência da execução orçamentária dos 79 (setenta e nove) municípios do estado de Mato Grosso do Sul, realizou-se uma pesquisa descritiva, por meio de análise documental, com o auxílio de ferramentas quantitativas. Para tanto, desenvolveu-se uma escala de avaliação da transparência pública ativa dos municípios, conforme as informações disponibilizadas pelos seus sítios eletrônicos. A partir dos resultados apresentados, observou-se que não há uma diferença significativa da transparência ativa de acordo com a população ou com o montante de despesas dos municípios. Por fim, notou-se que a maioria deles ainda apresentam taxas de transparência incipientes, aquém das obrigações preconizadas pela Lei de Acesso à Informação.
Bystander effect formation in the sprout root meristem in condition of irradiated and non-irradiated seeds joint growing
Authors: German O. Yu.
Published: March 2020

Aim. To study the possibility of bystander effect inducing in the joint germination in a common aqueous environment of irradiated and non-irradiated seeds. Methods. Cytogenetic methods including mitotic activity level estimation, accounting for the number of nucleoli, and methods for statistical analysis of the data. Results. The effect of radiation stimulation with a dose of 40 Gy was dependent on the variety, and the effect of gamma radiation at a dose of 200 Gy reduced the level of mitotic activity in comparison with the control in both varieties. Mitotic indices increase in the meristem of non-irradiated seeds while growing with irradiated one. Conclusions. The bystander effect is formed on the organismic level under condition of growing of irradiated and non-irradiated seeds in a common aquatic environment and it is ma-nifested as an increase in the level of meristem cells proliferation and in the intensification of synthetic processes. The addition of irradiated with a high dose of gamma radiation seeds to the non-irradiated seeds induces an increase in mitotic activity largely than the addition of seeds irradiated with a relatively small dose.

Keywords: bystander effect, radiation stimulation, mitotic activity, nucleoli.
Characteristics of Chernigov’s population by the presentation data
Authors: Gorpynchenko M. Yu. , Atramentova L. O.
Published: March 2020

Aim. The population of the world has a complex hierarchical population structure. At the heart of this hierarchy are elementary populations. Methods. Using the quasigenetic marker – surnames – the genetic indices of the population of the Chernihiv region of the rayon and oblast levels were obtained, comparing them with similar indices of the populations of the Poltava (central) and Odessa (borderline) populations. Results. The Chernihiv region is borderline, but the base indicator is an index of isonomy higher (I = 4.57 × 10-4) than in the border region of Odessa, and also more than in the central - Poltava population. The index of inbreeding in the Chernihiv population is Fst = 11.43 × 10-5. The indicator of migration efficiency was v = 17.37 × 10-4 and occupies an intermediate position between the Poltava and Odessa populations. The value of the index H as a whole for the region is 13.24, the index of redundancy of the distribution of names is R = 34.67. Conclusions. The study complements the picture of genetic peculiarity of Ukrainian populations. The genetic characteristics of the Chernihiv population, its difference from the previously studied central Poltava and border area of Odessa, are explained by the peculiarity of the location of the Chernihiv region, the features of the landscape, the other ethnic and civic belonging of the geographically contacting peoples. Keywords: quasigenetic markers, population, isonymy, inbreeding, migration.
Modeling of Parkinson's disease on D. melanogaster: oxidative stress and the role of isogenization of transgenic lines
Authors: Dronska Kh. A. , Yavdyk Kh. M. , Stasyk O. H. , Matiytsiv N. P.
Published: March 2020

Aim. Oxidative stress (OS) is considered one of the main factors that leads to the degeneration of dopamine neurons in Parkinson's disease (PD). The purpose of the work was to test sensitivity to the conditions of the OS of D. melanogaster individuals with expression of human alpha-synuclein in neurons UAS-SNCA/elavGal4; and establish the role of the isogenization of the lines derived from stock collections in the study of this phenotype. Methods. For the isogenyzation of the line, we conducted five generations of sequential crossings of individuals with insertion of human alpha synuclein gene into the w1118 line. A 4-day test using H2O2 as prooxidant was used to test the sensitivity to OS conditions. Results. Individuals with expression of alpha-synuclein gene in neurons were characterized by statistically significant sensitivity to OS conditions, compared with controls. Also, there was a significant difference in the degree of sensitivity to the OS in the second day of the experiment in individuals before and after the isogenization of the effector line. Conclusions. Hypersensitivity to the OS is detected as a specific phenotype under conditions of expression of human alpha-synuclein in Drosophila neurons. The importance of the isogenization of transgenic lines for the characterization of the stress susceptibility phenotype is established.Keywords: Drosophila melanogaster, alpha-synuclein, oxidative stress, isogenization.
PTEN-dependent changes of gene expressions in human prostate tumors
Authors: Gerashchenko G. V. , Grygoruk O. V. , Mevs L. V. , Kashuba V. I.
Published: March 2020

Aim. To determine the levels of PTEN relative expression (RE) and the possible effects of its loss on some tumor-associated genes and histological characteristics of human prostate tumors. Methods. PTEN RE and 46 genes were identified in 37 pairs of adenocarcinoma and conventionally normal tissues (CNT) and 20 adenomas by real time PCR. Results. We are shown PTEN RE decreasing in the adenocarcinoma group as well as in CNTs compared with adenomas. 54 % of adenocarcinomas, 48.6 % of CNT and 25 % of adenomas were detected with low PTEN RE. We have found 9 of 46 differentially expressed genes in groups with different levels of PTEN RE (ESR1, GCR, KRT18, MMP2, MMP9, SRD5A2, VIM, PCA3, HOTAIR). It was revealed some histological characteristics correlated with PTEN RE in prostate adenocarcinomas. Conclusions. PTEN aberrations in prostate tumors indicate that PTEN associated changes already have been occurred at the benign tumors. We have shown some tumor histological characteristics associated with PTEN expression changes.Keywords: prostate tumors, relative gene expression, PTEN, EMT, histological characteristics.
Optimization of ChIP protocol for investigation of canonical Wnt target genes expression in new born heart
Authors: Piven O. O.
Published: March 2020

Aim. In present work we have focused on chromatinimmunoprecipitation (ChIP) protocol with next qPCR optimization for analysis of canonical Wnt target genes regulation by b‑catenin and plakoglobin in new born heart. Methods. In study we have used the new born hearts tissue at the age of 3 days after birth (P3). We have analyzed hearts of control mice and mice with heterozygous knockout of β-catenin. We have proceeded ChIP protocol based on standard protocols in our modifications. ChIP results validated with qPCR. Results. We have adopted ChIP protocol for investigation of signalling function of β-catenin and plakoglobin as well as for identification of β-catenin and plakoglobin specific binding genomic region in new born heart. Conclusions. We have shown that plakoglobin is able to compensate the signaling function of β-catenin and binds with similar genomic region under β-catenin haploinsufficiency conditions.

Keywords: β-catenin, plakoglobin, Wnt signaling, gene expression, heart, chromatinimmunoprecipitation, ChIP.
Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene
Authors: Honcharuk O. M. , Dubrovna O. V.
Published: March 2020

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes.

Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.
The combination of NO donor and ferulic acid effect on the elicitation of Triticum aestivum tolerance against Septoria tritici
Authors: Zhuk I. V. , Dmitriev A. P. , Lisova G. M. , Kucherova L. O.
Published: March 2020

Aim. The aim is to research the ability of enhancing ferulic acid effect as a biotic elicitor to induce tolerance of winter wheat plants against Septoria tritici Rob et Desm. leaf blotch infection by addition donor of NO signal molecule. Methods. Content of endogenous H2O2 was measured in wheat leaves (cv. Oberig myronivskij and Svytanok myronivskij). The extent of disease development, morphometric parameters and yield structure were analyzed. Results. It is shown that combination of treatment by ferulic acid with NO donor reduced the disease symptoms on 1–2 points. The level of endogenous hydrogen peroxide increased on 27 % in cv. Svytanok myronivskij. Conclusions. The data obtained suggest that combination of ferulic acid with donor NO could be used as more effective combination than biotic elicitor. They decreased the degree of lesions in leaf area caused by Septoria tritici Rob et Desm. leaf blotch infection and stimulated the growth of wheat plants. The analyze of biochemical mechanisms revealed that system resistance of wheat plants is induced via activation of antioxidant protection. Keywords: ferulic acid, NO, biotic elicitors, induced resistance, Triticum aestivum L., Septoria tritici Rob et Desm.
Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro
Authors: Zambriborshch I. S. , Shestopal O. L. , Boyko M. S.
Published: March 2020

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments.

Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.
Applying the nanobiotechnological methods for optimize the in vitro cultivation technology for cows oocytes
Authors: Kovtun S. I. , Zyuzyun A. B. , Shcherbak O. V. , Trotskiy P. A.
Published: March 2020

Aim. Investigated effect nanomaterial of highly dispersed ultra fine silica (UFS) by carbohydrate – sucrose (UFS/sucrose) on the effectiveness of meiotic maturation cows oocytes in vitro. Methods. The fresh and frozen – thawed cow oocyte-cumulus complex (OCC) was divided into four groups: three experimental in which the cultivation was carried out in a medium containing of 0, 1; 0, 01 and 0,001 % UFS/sucrose and control without adding nanomaterial. Results. It was concluded that the addition of UFS/sucrose in 0.001 % concentration is effective for elevation level of oocytes maturation and provides reception of 76,8% oocytes that induced the metaphase II of meiosis. Adding carbohydrate – sucrose (UFS/sucrose) in 0.001 % concentration to the culture medium frozen – thawed cow generative cells, make positive effect on in vitro fertilization and provide embryos quantity enhancement to 33.3 %. Conclusions. Addition of UFS/sucrose in 0.001 % concentration to the culture medium have increase effect and promote level in vitro maturation of cows oocytes rising to 76.8 %. Usage of UFS/sucrose in 0.001 % concentration as part of in vitro culture medium for cows oocyte-cumulus complex conduce rising quantity of cattle embryos to 33.3 % after in vitro fecundation frozen – thawed and maturation oocytes.

Keywords: oocytes, in vitro culture, embryos, nanomaterial, ultra fine silica (UFS).
DNA certification of varieties and hybrids of perennial grass crops
Authors: Kondratskaya I. P. , Yukhimuk A. N. , Chizhik O. V. , Reshetnikov V. N. , Stolepchenko V. A. , Vasko P. P.
Published: March 2020

Aim. The aim of this study was to carry out passportization of cultivars, varieties and hybrids of perennial grass crops: Alopecurus protensis, Agropyron cristatum, Lolium perenne, Lolium miltiflorum, Festuca protensis, Festuca arundinasea, hybrids Festulolium. Methods. The perennial grasses selection was carried out by embryo culture according to methodical recommendations. For the molecular-genetic passportization of perennial grasses varieties the polymorphous primers have been selected – 9 RAPD primers and 15 ISSR primers. Results. Multilocus DNA labeling have allowed to differentiate all the genotypes being studied, to develop and to create the unique profiles for each of them and to calculate the genetic distance of congeniality/distances. On the base of RAPD/ISSR, SCoT spectra the 14 molecular-genetic passports of perennial grass cops have been obtained for the first time.

Keywords: grass crops, molecular-genetic passport, DNA, PCR, multipoint mapping RAPD/ISSR spectra.
A high-throughput virtual screening in Grid for new tubulin-targeted inhibitors of plant fungal pathogens
Authors: Karpov P. A. , Demchuk O. M. , Rayevsky O. V. , Ozheredov S. P. , Spivak S. I. , Samofalova D. O. , Blume Ya. B.
Published: March 2020

Aim. To select new tubulin-targeted inhibitors of plant fungal pathogens based on results of high-throughput virtual screening in Grid. Methods. Protein and ligand spatial structure modelling (I-Tasser, Grid), design and virtual screening ligands library (UCSF Dock 6, Grid), molecular docking (CCDC Gold), molecular dynamics simulation (Gromacs, Grid). Results. 240 structural models of tubulin molecules (82 α-, 111 β- and 47 γ-tubulin) from 62 species of phytopathogenic fungi were constructed. It was found that imidazole ligands, demonstrate strongest affinity to α- and β-tubulin. It was found that among α-, β- and γ-tubulin, taxol binding site of β-tubulin possess the strongest potential as the fungicidal drugs target. It was selected 50 leader compounds: 23 with affinity for GTP/GDF-exchange site and 27 with affinity for taxol-binding site. Conclusions. It was found, that in phytopathogenic fungi, taxol binding site of β-tubulin are the main fungicid drug target (in compare to other tubulin site or isotype). The highest affinity was predicted for the compounds F0478-0219, F0478-0166 and β-tubulin from Puccinia graminis f. sp. Tritici, as well as for the compound F0478-0385 and β-tubulin from Magnaporthe oryzae.

Keywords: pathogenic fungi, fungicides, tubulin, virtual screening, Grid.
The course of anthropogenetics in a classical university
Authors: Atramentova L. A.
Published: March 2020

The article describes the principles of teaching the course of anthropogenetics in a classical university, taking into account the needs of various branches of science and practical activity: medicine, pedagogy, psychology, criminology, pharmacology, etc. The main examples of the most frequent mistakes in the planning and organization of anthropogenetic research are given. These examples concern the approaches and methods of genetics in solving scientific and practical problems associated with human heredity. The most outstanding achievements of Kharkov anthropogenetic school as the center of systematic research in the field of human genetics in Ukraine are described. The description of the training course of anthropogenetics, taught at the Biological Faculty of the V.N. Karazin Kharkov National University is given.

Keywords: anthropogenetics.
Methodological problems of biotic diagnostics of antropogenic transformation of forest ecosystems at the levels of organization of life
Authors: Lavrov V. V. , Blinkova O. I.
Published: March 2020

Aim. The purpose of the study was to identify and systematize the methodological problems of the diagnosis of anthropogenic transformation of forests by the levels of organization of life. Methods. Theoretical methods of system-structural, retrospective, comparative analysis were combined with special methods of forestry, synecology, mycology, ornithology. Results and conclusions. The study of higher levels of biological organization, integral characteristics of ecosystems, reflecting the state of their integrity and development should be given priority in bio-diagnostics of forest ecosystems. Studies at lower levels of the hierarchy of levels without proper alignment with the composition and state of higher subsystems can lead to false conclusions. Studying at the basic levels of biological organization gives the opportunity to adhere to the principles of emergence, functional integration and hierarchical organization of the biosphere and its homeostasis systems.

Keywords: biological organization, complex of biological structures, forest ecosystem, transformation.
Do not forget the name of V.P. Efroimson (1908–1989)
Authors: Etkalo E. N.
Published: March 2020

In 2018, 110 years have passed since the birth of Vladimir Pavlovich Efroimson (1908–1989). Efroimson studied at the biological department of Moscow University, in 1929 was expelled for the support of S.S. Chetveryakov. In December 1932 he was arrested. Since 1935 works in the Central Asian Institute of Sericulture, but in August 1937 he was dismissed from the Institute for his performance in defense of "Mendelism-Morganism". In the same year he was declared an enemy of the people, and he leaves for Ukraine. During this period he wrote a book on "Genetics and breeding of the silkworm". In May 1941, Efroimson defended his Ph.D. thesis at Kharkov University and went to the front. Rewarded by the Red Star and Patriotic War II degree. After the war, Efroimson returned to Kharkov University as an assistant professor of the Department of Darwinism and Genetics. In 1947 he defended his doctoral dissertation on the problems of evolutionary genetics and breeding of the silkworm. In 1951 he was sentenced to 10 years in prison. After rehabilitation he returns to Moscow and works as a bibliographer. In 1967, he received the title of professor and worked in the Institute of Psychiatry. He published a number of monographs, among them "Genetics of Oligophrenia, Psychoses and Epilepsy". His well-known books on medical genetics, anthropogenetics, gene determination of intelligence and behavior: "Genius and Genetics", "Genetics of Ethics and Aesthetics", "Pedagogical Genetics".

Keywords: genetics, history of science, V.P. Efroimson.
Efficiency of the use of mutations, induced on radiation-contaminated areas, when improving winter wheat cultivars
Authors: Yakymchuk R. A.
Published: March 2020

Aim. Breeding of Triticum aestivum L. cultivars requires the development of generically diversified primary material. Induced mutagenesis plays an important role in the solution of the issue.  Mutagenic activity of radionuclide contaminations of the alienation zone of ChNPP and industrial facilities of uranium manufacture was studied to determine the efficiency of their use when developing the material of winter wheat which is valuable for breeding. Methods. The selection of mutants was done using the methods of recording and singling out of visible mutations, phenological observations, analysis of biometric indicators, determination of total yield capacity and its components. Results. Chronic effect of the radiation of radionuclide contaminations of the alienation zone of ChNPP and the territory of uranium ore mining results in the increase of mutation variability of winter wheat by 8–14.9 and 9.8–12.6, respectively. Mutation spectrum included 24.29–49.34 % of those valuable for breeding. Productive mutants, which exceeded primary cultivars in crop capacity by 2.7–6.9 %, were developed. Mutant samples, whose high yield capacity was supplemented with grain quality enhancement or its maintenance at the level of a primary form, were identified. Conclusions. The expansion of genetic variety of primary breeding material due to mutagenesis, induced by radionuclide contaminations, creates the opportunities for its use in hybridization aimed at the implementation of breeding-genetic programs of the improvement of wheat cultivars.

Keywords: Triticum aestivum L., radionuclide contamination, mutation variability, valuable for breeding mutations, productive forms.
The effect of various NaCl concentrations on morphophysiological properties of bread wheat sprouts (Triticum aestivum L.)
Authors: Gasimova F. I. , Khanishova M. A. , Taghiyeva K. R. , Azizov I. V.
Published: March 2020

Aim. The effects of various NaCl concentrations on germination energy, germination percentage of seeds and morpho-physiological indices of seedlings have been determined in bread wheat (Triticum aestivum L.) varieties. Methods. Wheat seeds were germinated at 0, 100, 150, 200 mM concentrations of NaCl using the roll method. Rezults. The delay of the growth of seedlings and root system was observed with increasing salt concentrations in all samples. The effect of various salt concentrations on separate varieties was diifferent. Conclusions. The highest indices of the development of coleoptiles and root system and the highest amount of chlorophyll were detected at 150 and 200 mM concentrations of NaCl in the Mirbashir-128 variety. The Bezostaya and Nurlu-99 varieties manifested medium tolerance, while the Sheki-1 variety was relatively less tolerant.

Keywords: NaCl, wheat varieties, germination energy, germination percentage, chlorophyll.
Molecular genetic test system for diagnosis of x-linked agammaglobulinemia
Authors: Dybkov M. V. , Stepanovskyy Yu. S. , Chernyhova L. I. , Volokha A. P. , Hilfanova A. M. , Bondarenko A. V. , Lapiy F. I. , Dybkova K. M. , Telegeev G. D.
Published: March 2020

Aim. Primary immunodeficiencies are disorders in which immune system is missing or does not function normally due to genetic disorders. X-linked agammaglobulinemia (XLA) is a rare genetic disorder in which don’t generate of mature B cells, which manifests as a complete or near-complete lack of proteins called gamma globulins, including antibodies, in their bloodstream. The BTK protein is the key regulator of β-cell maturation, so mutations in the BTK gene lead to hereditary agammaglobulinemia. Detection of mutations of the BTK gene is important for confirmation of the diagnosis in patients with XLA. Methods. DNA and RNA extraction, RT-PCR, direct Sanger sequencing of PCR fragments. Results. An analysis of blood samples from patients with XLA using a proposed test system was performed. It has been shown that the proposed test system allows for the initial screening of mutations in the BTK gene. Conclusions. The test system for the detection of mutations in patients with X-linked agammaglobulinemia, based on using of reverse transcriptase PCR and direct sequencing, was developed and tested on clinical samples. This system is proposed for the initial screening of BTK gene mutations inUkraine. Keywords: X-linked agammaglobulinemia, XLA, BTK, mutation, RT-PCR, primary immunodeficiency.
Renewing DNA integrity by cell repair systems
Authors: Lukash L. L.
Published: March 2020

The four of six main cell repair systems have been reviewed. Some of them have been studied by Nobel laureates of 2015 year in the field of chemistry. The common title of their work is “For investigation of DNA repair mechanisms”. Numerous primer damages of DNA induced by exogenous and endogenous factors are corrected by complex cell systems including repair enzymes and other proteins. Mutational changes in the genes coding the repair enzymes are the most dangerous because they dramatically increase mutation frequency in pro- and eukaryotic cells. But due to the constant and correct action of cell repair enzymes DNA integrity is renewing, that is why the organisms on our planet are still alive and functionally active.

Keywords: DNA repair, reparative enzymes, pro- and eukaryotic cells, primer damages, mutations.
Aspects of the study of multi-level mechanisms of human adaptation to the extreme conditions of Antarctica
Authors: Moiseyenko Ye. V. , Rozova K. V. , Yanchiy R. I.
Published: March 2020

Aim. Study of mechanisms of adaptation of organism functions, including molecular genetic level, to extreme conditions of professional activity in Antarctica. Methods. Survey of Antarctic winterers with clinical, functional, psychological and molecular genetic research methods. Results. The negative dynamics of psychoneurotic symptoms in winterers is established, which dictates the necessity of continuous improvement of the method of medical – psychophysiological selection, the use of additional technologies of examination and training, as well as further research aimed at identifying biomarkers that are sensitive to the impact of a long expedition in extreme conditions in the Antarctic. The allelic polymorphism of the HIF-1α gene affects hypoxic induced changes in the ultrastructure and function of the blood cells, which can serve as a marker for the formation of adaptive reactions. Conclusions.The results of the research indicate that the study of adaptation and dysadaptation mechanisms at all levels of the functioning of the organism, including molecular genetic, is of prime importance in the system of prevention of pathology of Antarctic winterers. The results of research at the Ukrainian Antarctic station can be used in practical medicine and taken into account when formulating proposals to improve the international system of safe human habitation in Antarctica. Keywords: molecular genetic, psychophysiological selection, winterers, Antarctic.
Comparative cytogenetic analysis of early reproductive losses depending of maternal age
Authors: Tkach I. R. , Huleyuk N. L. , Zastavna D. V. , Sosnina K. O. , Bezkorovaina G. M. , Helner N. V.
Published: March 2020

Aim. Human reproduction characterized by a high incidence of aneuploidies. Approximately 99 % of conceptions with anomalies of karyotype terminate of pregnancy loss, mainly during the first 14 weeks of intrauterine development. The frequency and spectrum of karyotype anomalies in the chorionic villus of early reproductive losses were studied depending of maternal age. Methods. Banding cytogenetic and interphase mFISH with the centromeric probe panel for chromosomes 13, 14, 15, 16, 17, 18, 21, 22, X and Y were used. Results. The contribution of different karyotype abnormalities in the genesis of the early reproductive losses depends on the age of the woman, namely, with age significantly increases the proportion of aneuploidy due to autosomal trisomies and reduced contribution of polyploidy and gonosomal monosomy. The main autosomal trisomy in the material of lost pregnancies from women under the age of 35 is 16, 21 and 15, 22, 13 and 18 in order of decreasing frequency, and from older women 16, 15, 22, 21, 13 and 14. Conclusions. The structure and rate of karyotype anomalies in the material of lost pregnancies varies with maternal age.

Keywords: early reproductive loss, maternal age, karyotype abnormalities.
The frequency of genetic factors of thrombophilia in women with recurrent pregnancy losses
Authors: Chorna L. B. , Makukh H. V. , Zastavna D. V. , Prokopchuk N. V. , Helner N. V.
Published: March 2020

Aim. There is growing evidence that recurrent pregnancy losses (RPL) are associated with the presence of inherited thrombophilias but data are inconsistent. The present study aimed to assess the distribution of inherited risk factors of thrombophilia among women with RPL. Methods. We studied 68 women with RPL and 120 healthy women of control group, inhabitants of Western Ukraine. In all subjects the detection of genetic factors of thrombophilia were determined by polymerase chain reaction and restriction fragment length polymorphism method. Results. The prevalence of heterozygotes for FV 1691G/A among women with RPL and controls were: 12 % versus 4 %, respectively. Women heterozygosity for factor V Leiden was significantly more prevalent in the RPL group than in controls (OR 3.11, 95 % CI 1.02–9.46). Results showed that carriers of PAI-1 4G allele have increased odds on more than 2 times in comparison to the carriers of homozygous 5G5G genotypes. A significant relationship between allele variation 677T of MTHFR gene (OR 1.70, 95 % CI 1.09–2.67) and RPL was observed. Conclusions. Significance of 1691G/A mutation of V blood coagulation factor gene, alleles variations of 677T of MTHFR gene and 4G of PA1-1 gene in the structure of predisposition to RPL in group of west Ukrainian women was established.

Keywords: genetic factors, hereditary thrombophilia, molecular genetic testing, RPL.
Effects of genes controlling the plant development rates on the formation of individual productivity of wheat and soybean
Authors: Avksentiieva O. A. , Zubrich A. I. , Vasilchenko M. S. , Shulik V. V.
Published: March 2020

Aim. The aim of the work was to study the effects of genes controlling the plant development rates on the speed of transition to generative development and formation of individual productivity of isogenic lines of wheat (Triticum aestivum L.) and soybean plants (Glycine max (L.) Merr.) according to the genes. Methods. The experiments were carried out in the field conditions. The periods of sprouting and earing (PSE) of wheat, the periods of sprouting and flowering (PSF) and ripening (PSR) of soybean isogenic lines and the elements of the structure of individual productivity were determined. Results. It was shown that under conditions of a natural long day (16 hours), wheat plants with genotypes VRN-A1a, VRN-D1a, PPD-A1a and PPD-D1a more rapidly passed to the earing. In soybean isogenic lines, the presence of dominant alleles of the E-series genes increased the duration of phases of PSF and PSR. The maximum indicators of productivity elements were characterized by isogenic lines, developed at a faster rate. Conclusions. The genes controlling the development rates of soft wheat and soybean crops, defining the timing of the transition to generative development, determine their productivity.

Keywords: Triticum aestivum L., Glycine max (L.) Merr., VRN, PPD, EE genes, rates of development, productivity.
The resistance of chernozem soil microorganisms to soluble copper compounds
Authors: Havryliuk O. A. , Govorukha V. M. , Tashyrev A. B.
Published: March 2020

Аim. Determination of resistance of Ukrainian chernozem soil microorganisms to the influence of toxic copper(II). Methods. Content of copper-resistant microorganisms in chernozem was determined by counting colonies on a solid nutrient medium containing Cu(II). Resistance of microorganisms was determined by cultivation in the medium with concentration gradient of Сu2+. Results. Microorganisms resistant to toxic copper(II) by ultrahigh concentrations were shown to be present in chernozem soil. Microorganisms grew in the medium containing up to 500 mg/l Cu2+ (CuSO4 solution) and up to 10000 mg/l Cu2+ (in complex with citrate). Chelation of copper(II) with citrate was found to lead to increase of microbial resistance in 20 times. It was determined that a vanishingly small number of microorganisms resistant to toxic copper by ultrahigh concentrations can exist in a natural ecosystem. The number of viable microorganisms decreases with the increase in the content of Cu2+ that is described by the hyperbolic curve. Conclusions. The proposed methodological approach not only allows to isolate copper-resistant microorganisms from natural ecosystems of all geographic zones of the globe, but also avoid complex genetic transformations in order to obtain perspective genetically modified strains for further application in biotechnologies for purification of industrial wastewater.

Keywords: copperresistant microorganisms, chernozem soil of Ukraine, diversified microbial community, environmental biotechnologies.
Taxonomical position of pigmented yeast isolated from Antarctic ecosystems
Authors: Gladka G. V. , Romanovskaya V. O. , Belkova N. L. , Iungin O. S. , Tashyrev A. B.
Published: March 2020

Aim. To determine species composition and taxonomic position of extremotolerant Antarctic yeast. Methods. The objects of research were yeast from Antarctic soils and phytocenosis, which are stored in the Collection of Extremophilic Microorganisms at the Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiev. Yeast were grown on malt wort (рН 5.0–5.5, temperature 18–20°С). Isolation of genomic DNA was performed using a commercial kit DNA-sorb; amplification of DNA preparations – use of primers NL1 and NL4; phylogenetic analysis – construction of dendrograms showing the position of the studied strains among closely related and typical species. Results. The analysis have shown a high percentage of similarity (98.4–99.9 %) of 18S rRNA genes sequences of yeast Antarctic strains and yeast sequences from the database GenBank. It was found that yeast strains belonged to phylum Basidiomycota (genera Rhodosporidium, Rhodotorula) and Ascomycota (genus Exophiala). Conclusions. As it was shown by phylogenetic analysis, red yeast S33, S48, S182 clustered with Rhodotorula mucilaginosa; S14 – with Rhodosporidium diobovatum and can be attributed to these species. Extremotolerant black yeast S10, S36 and S237 refer to the species Exophiala nigra. Keywords:Antarctica, extremotolerant yeast, phylogenetic analysis, species composition.
Development and studying of Triticum aestivum L. material with introgressions from Aegilops biuncialis Vis.
Authors: Kozub N. A. , Sozinov I. A. , Bidnyk H. Ya. , Demianova N. A. , Sozinova A. I. , Karelov A. V. , Pylypenko L. A. , Blume Ya. B. , Sozinov A. A.
Published: March 2020

Aim. The aim of the study was to develop and study T. aestivum material with introgressions from Ae. biuncialis. Methods. Quantitative traits of F4 lines from crossing wheat with Crimean accessions of Ae. biuncialis were studied. SDS and APAG electrophoreses of storage proteins were used to identify alleles at the Glu-1 and Gli-1 loci, including introgressed ones. Results. F4 lines from crosses of wheat with Ae. biuncialis showed a wide range of yield traits. Some lines had a clavate spike and a hairy leaf blade. Using storage proteins as genetic markers the presence of chromosome 1U was identified among the progeny of plants analyzed; some of them had translocation of arm 1UL. Lines with introgressions of chromosome 1M were not revealed. Conclusions. T. aestivum lines with introgressions from Ae. biuncialis were developed without amphidiploid production. Lines with chromosome 1U were selected, including lines with translocations of arm 1UL.

Keywords: Triticum aestivum L., Aegilops biuncialis Vis., introgression.
Influence of various factors on the antiradical activity of Panax ginseng С. A. Meyer extracts
Authors: Reshetnikov V. N. , Shutava H. G. , Shysh S. N. , Gil T. V.
Published: March 2020

Aim. The aim of the work was to evaluate the anti-radical activity of extracts of ginseng's underground and overground organs Panax ginseng C.A. Mey, depending on various factors – age of plants, collection time and cultivation conditions. Methods. Extracts obtained from various plant organs were evaluated in the model system with cation-radicals ABTS. At the same time, the amount of extractive substances and phenolic compounds was determined. Results. Samples of leaves and roots of ginseng of 1, 2, 3, 5, 9 years of vegetation from the collection of the Central Botanical Garden of the National Academy of Sciences of Belarus were analyzed. It is established that extracts of ginseng leaves of P. ginseng can be considered as sufficiently effective anti-radical agents. Anti-radical activity of extracts of roots and leaves of ginseng differed significantly depending on the weather conditions of the growing season. Conclusions. It is shown that extracts of ginseng leaves differ by the maximum antiradical activity. Extracts of ginseng roots showed a much lower anti-radical activity in comparison with leaf extracts, which may be due to both a smaller amount of extractives extracted from water-ethanol extraction and their composition.

Keywords: ginseng, antiradical activity, phenolic compounds, extractives.
Development of caryological and molecular-genetic investigations of wetland and dryland populations of conifers
Authors: Sedel'nikova T. S.
Published: March 2020

Aim. On the basis of studies which carried out by various authors, regularities of karyotypic and genetic divergence of populations of conifers growing in wetlands and dry lands are analyzed. Methods. The karyological and molecular-genetic methods of analysis are used. Results. Data are obtained on possible ways of evolution of karyotypes, consisting in changes in the number of chromosomes, the size and morphology of chromosomes, the number of secondary constrictions in chromosomes, the spectrum of chromosome rearrangements, and the frequency of their occurrence, distinguishing wetland populations of conifers species from dry land ones. With the help of isozyme markers and markers of nuclear and cytoplasmic genomes differences in the genetic structure of conifers populations from wetlands and dry lands are revealed. Conclusions. The genotypic structure of conifers species in wetlands and dry lands, caused by different vector of natural selection in contrasting ecological conditions of growth, differs significantly, and their karyotypic and genetic differentiation corresponds to the level of populations and groups of populations.

Keywords: populations of conifers, wetlands, dry lands, karyological and molecular-genetic markers.