bluuebunny/crossref_metadata_2025 huggingface dataset:
Outcomes of Definitive Chemoradiotherapy for Stage IVa (T4b vs. N4) Esophageal Squamous Cell Carcinoma Based on the Japanese Classification System: A Retrospective Single-Center Study
Authors: Wada Yuki , Anbai Akira , Takagi Noriko , Kumagai Satoshi , Okuyama Eriko , Nanjo Hiroshi , Sato Yusuke , Motoyama Satoru , Hashimoto Manabu
Published: December 2020

The differences in prognoses or progression patterns between T4b non-N4 and non-T4b N4 esophageal squamous cell carcinoma post chemoradiotherapy (CRT) is unclear. This study compared the outcomes of CRT for stage IVa esophageal squamous cell carcinoma according to T/N factors. We retrospectively identified 66 patients with stage IVa esophageal squamous cell carcinoma who underwent definitive CRT at our center between January 2009 and March 2013. The treatment outcomes, i.e., progression patterns, prognostic factors, and toxicities based on version 5.0 of the National Cancer Institute Common Terminology Criteria for Adverse Events, were studied. The patients (56 men and 10 women) had a median age of 67 (range: 37–87) years. The T/N classifications were T4b non-N4 (28/66), non-T4b N4 (24/66), and T4b N4 (14/66). Objective response was achieved in 57 patients (86.4%, (95% confidence interval, 74.6–94.1%)). There were no significant differences between the T/N groups in terms of overall survival, progression-free survival, and progression pattern. We found no significant differences in prognoses or progression patterns among patients with T4b non-N4, non-T4b N4, and T4b N4 esophageal squamous cell carcinoma. Thus, it seems impractical to modify CRT regimens based on T/N factors.
piNET–An Automated Proliferation Index Calculator Framework for Ki67 Breast Cancer Images
Authors: Geread Rokshana Stephny , Sivanandarajah Abishika , Brouwer Emily Rita , Wood Geoffrey A. , Androutsos Dimitrios , Faragalla Hala , Khademi April
Published: December 2020

In this work, a novel proliferation index (PI) calculator for Ki67 images called piNET is proposed. It is successfully tested on four datasets, from three scanners comprised of patches, tissue microarrays (TMAs) and whole slide images (WSI), representing a diverse multi-centre dataset for evaluating Ki67 quantification. Compared to state-of-the-art methods, piNET consistently performs the best over all datasets with an average PI difference of 5.603%, PI accuracy rate of 86% and correlation coefficient R = 0.927. The success of the system can be attributed to several innovations. Firstly, this tool is built based on deep learning, which can adapt to wide variability of medical images—and it was posed as a detection problem to mimic pathologists’ workflow which improves accuracy and efficiency. Secondly, the system is trained purely on tumor cells, which reduces false positives from non-tumor cells without needing the usual pre-requisite tumor segmentation step for Ki67 quantification. Thirdly, the concept of learning background regions through weak supervision is introduced, by providing the system with ideal and non-ideal (artifact) patches that further reduces false positives. Lastly, a novel hotspot analysis is proposed to allow automated methods to score patches from WSI that contain “significant” activity.
A Comprehensive Evaluation of Associations Between Routinely Collected Staging Information and The Response to (Chemo)Radiotherapy in Rectal Cancer
Authors: Hammarström Klara , Imam Israa , Mezheyeuski Artur , Ekström Joakim , Sjöblom Tobias , Glimelius Bengt
Published: December 2020

Radiotherapy (RT) or chemoradiotherapy (CRT) are frequently used in rectal cancer, sometimes resulting in complete tumor remission (CR). The predictive capacity of all clinical factors, laboratory values and magnetic resonance imaging parameters performed in routine staging was evaluated to understand what determines an excellent response to RT/CRT. A population-based cohort of 383 patients treated with short-course RT (5 × 5 Gy in one week, scRT), CRT, or scRT with chemotherapy (scRT+CT) and having either had a delay to surgery or been entered into a watch-and-wait program were included. Complete staging according to guidelines was performed and associations between investigated variables and CR rates were analyzed in univariate and multivariate analyses. In total, 17% achieved pathological or clinical CR, more often after scRT+CT and CRT than after scRT (27%, 18% and 8%, respectively, p < 0.001). Factors independently associated with CR included clinical tumor stage, small tumor size (<3 cm), tumor level, and low CEA-value (<3.8 μg/L). Size or stage of the rectal tumor were associated with excellent response in all therapy groups, with small or early stage tumors being significantly more likely to reach CR (p = 0.01 (scRT), p = 0.01 (CRT) and p = 0.02 (scRT+CT). Elevated level of carcinoembryonic antigen (CEA) halved the chance of response. Extramural vascular invasion (EMVI) and mucinous character may indicate less response to RT alone.
Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
Authors: Kretschmer Rafael , Gunski Ricardo José , Garnero Analía del Valle , de Freitas Thales Renato Ochotorena , Toma Gustavo Akira , Cioffi Marcelo de Bello , Oliveira Edivaldo Herculano Corrêa de , O’Connor Rebecca E. , Griffin Darren K.
Published: December 2020

Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined conventional and molecular cytogenetic approaches to investigate chromosome homologies between chicken and the smooth-billed ani (Crotophaga ani). Our results demonstrate extensive chromosome reorganization in C. ani, with interchromosomal rearrangements involving macro and microchromosomes. Intrachromosomal rearrangements were observed in some macrochromosomes, including the Z chromosome. The most evolutionary notable finding was a Robertsonian translocation between the microchromosome 17 and the Z chromosome, a rare event in birds. Additionally, the simple short repeats (SSRs) tested here were preferentially accumulated in the microchromosomes and in the Z and W chromosomes, showing no relationship with the constitutive heterochromatin regions, except in the W chromosome. Taken together, our results suggest that the avian sex chromosome is more complex than previously postulated and revealed the role of microchromosomes in the avian sex chromosome evolution, especially cuckoos.
Cellular, Molecular and Biochemical Impacts of Silver Nanoparticles on Rat Cerebellar Cortex
Authors: Mohamed Eman M. , Kattaia Asmaa A. A. , Abdul-Maksoud Rehab S. , Abd El-Baset Samia A.
Published: December 2020

Background: The excessive exposure to silver nanoparticles (Ag-NPs) has raised concerns about their possible risks to the human health. The brain is a highly vulnerable organ to nano-silver harmfulness. The aim of this work was to evaluate the impacts of Ag-NPs exposure on the cerebellar cortex of rats. Methods: Rats were assigned to: Control, vehicle control and Ag-NP-exposed groups (at doses of 10 mg and 30 mg/kg/day). Samples were processed for light and electron microscopy examinations. Immunohistochemical localization of c-Jun N-terminal kinase (JNK), nuclear factor kappa beta (NF-κB) and calbindin D28k (CB) proteins was performed. Analyses of expression of DNA damage inducible transcript 4 (Ddit4), flavin containing monooxygenase 2 (FMO2) and thioredoxin-interacting protein (Txnip) genes were done. Serum levels of inflammatory cytokines were also measured. Results: Ag-NPs enhanced apoptosis as evident by upregulation of Ddit4 gene expressions and JNK protein immune expressions. Alterations of redox homeostasis were verified by enhancement of Txnip and FMO2 gene expressions, favoring the activation of inflammatory responses by increasing NF-κB protein immune expressions and serum inflammatory mediator levels. Another cytotoxic effect was the reduction of immune expressions of the calcium regulator CB. Conclusion: Ag-NPs exposure provoked biochemical, cellular and molecular changes of rat cerebellar cortex in a dose-dependent manner.
Perylene Imide-Based Optical Chemosensors for Vapor Detection
Authors: Zhang Miao , Shi Jiangfan , Liao Chenglong , Tian Qingyun , Wang Chuanyi , Chen Shuai , Zang Ling
Published: December 2020

Perylene imide (PI) molecules and materials have been extensively studied for optical chemical sensors, particularly those based on fluorescence and colorimetric mode, taking advantage of the unique features of PIs such as structure tunability, good thermal, optical and chemical stability, strong electron affinity, strong visible light absorption and high fluorescence quantum yield. PI-based optical chemosensors have now found broad applications in gas phase detection of chemicals, including explosives, biomarkers of some food and diseases (such as organic amines (alkylamines and aromatic amines)), benzene homologs, organic peroxides, phenols and nitroaromatics, etc. In this review, the recent research on PI-based fluorometric and colorimetric sensors, as well as array technology incorporating multiple sensors, is reviewed along with the discussion of potential applications in environment, health and public safety areas. Specifically, we discuss the molecular design and aggregate architecture of PIs in correlation with the corresponding sensor performances (including sensitivity, selectivity, response time, recovery time, reversibility, etc.). We also provide a perspective summary highlighting the great potential for future development of PIs optical chemosensors, especially in the sensor array format that will largely enhance the detection specificity in complexed environments.
Opioid-Induced Respiratory Depression in Pediatric Palliative Care Patients with Severe Neurological Impairment—A Scoping Literature Review and Case Reports
Authors: Mauritz Maximilian David , Hasan Carola , Dreier Larissa Alice , Schmidt Pia , Zernikow Boris
Published: December 2020

Pediatric Palliative Care (PPC) addresses children, adolescents, and young adults with a broad spectrum of underlying diseases. A substantial proportion of these patients have irreversible conditions accompanied by Severe Neurological Impairment (SNI). For the treatment of pain and dyspnea, strong opioids are widely used in PPC. Nonetheless, there is considerable uncertainty regarding the opioid-related side effects in pediatric patients with SNI, particularly concerning Opioid-Induced Respiratory Depression (OIRD). Research on pain and OIRD in pediatric patients with SNI is limited. Using scoping review methodology, we performed a systematic literature search for OIRD in pediatric patients with SNI. Out of n = 521 identified articles, n = 6 studies were included in the review. Most studies examined the effects of short-term intravenous opioid therapy. The incidence of OIRD varied between 0.13% and 4.6%; besides SNI, comorbidities, and polypharmacy were the most relevant risk factors. Additionally, three clinical cases of OIRD in PPC patients receiving oral or transdermal opioids are presented and discussed. The case reports indicate that the risk factors identified in the scoping review also apply to adolescents and young adults with SNI receiving low-dose oral or transdermal opioid therapy. However, the risk of OIRD should never be a barrier to adequate symptom relief. We recommend careful consideration and systematic observation of opioid therapy in this population of patients.
Growth of Si3N4 Thin Films on Si(111) Surface by RF-N2 Plasma Nitriding
Authors: Chen Wei-Chun , Chen Sheng , Yu Tung-Yuan , Su James , Chen Hung-Pin , Lin Yu-Wei , Cheng Chin-Pao
Published: December 2020

Ultra-thin Si3N4 films were grown on Si(111) surface by radio frequency (RF)-N2 plasma exposure at 900 °C with 1–1.2 sccm of a flux of atomic nitrogen. We discuss the effect of various conditions such as N2 flow rate, nitriding time and RF power on the optical, chemical, and structural properties of a nitrided Si3N4 layer. The optical properties, surface morphology and chemical composition are investigated by using ellipsometry, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). Cross-sectional TEM images show that an RF power of 350 W induced some damage to the Si(111) surface. The thickness of nitrided Si3N4 was measured to be about 5–7 nm. XPS results shown that the binding energy of Si 2p3/2 located at 101.9 ± 0.1 eV is attributed to the Si–N bonds in the Si3N4 compound. Smooth Si3N4 ultra-thin films were obtained at a nitridation time close to 1 h with an RF power of 300 W, with a measured refractive index (n) nearly to 1.88 at 632 nm. The increase in refractive index with decreased RF-plasma power and nitrogen flow rate is probably attributed to the change in the stoichiometry of the film and less surface damage.
Teaching Mathematics at Distance: A Challenge for Universities
Authors: Cassibba Rosalinda , Ferrarello Daniela , Mammana Maria Flavia , Musso Pasquale , Pennisi Mario , Taranto Eugenia
Published: December 2020

The focus of this research is how Sicilian state university mathematics professors faced the challenge of teaching via distance education during the first wave of the COVID-19 pandemic. Since the pandemic entered our lives suddenly, the professors found themselves having to lecture using an e-learning platform that they had never used before, and for which they could not receive training due to the health emergency. In addition to the emotional aspects related to the particular situation of the pandemic, there are two aspects to consider when teaching mathematics at a distance. The first is related to the fact that at university level, lecturers generally teach mathematics in a formal way, using many symbols and formulas that they are used to writing. The second aspect is that the way mathematics is taught is also related to the students to whom the teaching is addressed. In fact, not only online, but also in face-to-face modality, the teaching of mathematics to students on the mathematics degree course involves a different approach to lessons (as well as to the choice of topics to explain) than teaching mathematics in another degree course. In order to investigate how the Sicilian State university mathematics professors taught mathematics at distance, a questionnaire was prepared and administered one month after the beginning of the lockdown in Italy. Both quantitative and qualitative analyses were made, which allowed us to observe the way that university professors have adapted to the new teaching modality: they started to appropriate new artifacts (writing tablets, mathematical software, e-learning platform) to replicate their face-to-face teaching modality, mostly maintaining their blackboard teacher status. Their answers also reveal their beliefs related to teaching mathematics at university level, noting what has been an advantageous or disadvantageous for them in distance teaching.
Techno-Economic Investigation of an Integrated Boiler–Solar Water Heating/Cooling System: A Case Study
Authors: Al-Smairan Mohammad , Shawaqfah Moayyad , AlMomani Fares
Published: December 2020

With the increase in oil prices, developing nations end up paying expensive electricity and heating bill. This leading study investigates the experimental development of a new energy-saving system by integrating a solar water heater and solar cooling absorption cycle with a conventional boiler for domestic hot water and heating purposes. The heating and cooling load calculations for space heating of the building were calculated using TRNSYS 14.1 computer software and the results were used in calculating the energy-saving value. A 65 flat plate solar collector-chiller system with a total surface area of 130 m2 was integrated with the boiler and used to supply heating and cooling for a three-story building (1500 m2) in Al Bayt University, Jordan. The integrated system helped to save energy, reduced the emission of CO2 into the atmosphere, supplied hot water, and space heating/cooling requirements to the building year-round, and reduced the overall energy cost of heating and cooling by 55% and 48%, respectively. Moreover, the techno-economic analysis showed that the payback period of the combined system with a total cost of $18,650 is roughly 2.5-year. The solar water heating/cooling system has the potential to provide more than 50% of the house energy demand free of charge with a significant reduction in carbon footprint.
Classification of Measures for Dealing with District Heating Load Variations—A Systematic Review
Authors: Djurić Ilić Danica
Published: December 2020

The highly varying character of district heating (DH) demand results in low capacity utilization of the DH plants, as well as increased use of fossil fuels during peak demand. The aim of this study is to present an overview and a comprehensive classification of measures intended to manage these load variations. A systematic literature review was conducted based on previously defined search strings as well as inclusion and exclusion criteria. Two scientific databases were used as data sources. Based on 96 detected publications, the measures were categorized as (1) complementing DH production in heat-only boilers (HOBs), or geothermal or booster heat pumps (HPs) (usually controlled by the DH company), (2) thermal energy (TE) storage in storage units or in the network (controlled by the company), and (3) demand side measures, which can be strategic demand increase, direct demand response (DR), or indirect DR. While the company has control over direct DR (e.g., thermal storage in the thermal mass of the buildings), indirect DR is based on communication between the customer and the company, where the customer has complete control. The multi-disciplinary nature of this topic requires an interdisciplinary approach.
Forecasting Volatility of Energy Commodities: Comparison of GARCH Models with Support Vector Regression
Authors: Fałdziński Marcin , Fiszeder Piotr , Orzeszko Witold
Published: December 2020

We compare the forecasting performance of the generalized autoregressive conditional heteroscedasticity (GARCH) -type models with support vector regression (SVR) for futures contracts of selected energy commodities: Crude oil, natural gas, heating oil, gasoil and gasoline. The GARCH models are commonly used in volatility analysis, while SVR is one of machine learning methods, which have gained attention and interest in recent years. We show that the accuracy of volatility forecasts depends substantially on the applied proxy of volatility. Our study confirms that SVR with properly determined hyperparameters can lead to lower forecasting errors than the GARCH models when the squared daily return is used as the proxy of volatility in an evaluation. Meanwhile, if we apply the Parkinson estimator which is a more accurate approximation of volatility, the results usually favor the GARCH models. Moreover, it is difficult to choose the best model among the GARCH models for all analyzed commodities, however, forecasts based on the asymmetric GARCH models are often the most accurate. While, in the class of the SVR models, the results indicate the forecasting superiority of the SVR model with the linear kernel and 15 lags, which has the lowest mean square error (MSE) and mean absolute error (MAE) among the SVR models in 92% cases.
Comparison of Pyrolysis Liquids from Continuous and Batch Biochar Production—Influence of Feedstock Evidenced by FTICR MS
Authors: Buss Wolfram , Hertzog Jasmine , Pietrzyk Julian , Carré Vincent , Mackay C. Logan , Aubriet Frédéric , Mašek Ondřej
Published: December 2020

Bio-oils from biomass pyrolysis can be a resource for upgrading to chemicals or fuels. Here, for the first time, we compare the composition of bio-oils produced from two feedstocks (wheat straw, softwood) in pyrolysis units of different mode of operation (continuous—rotary kiln vs. batch) using Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) in different ionization modes (APPI (+), ESI (+/−)). Our results demonstrate that the pyrolysis unit design had only a minor influence on the composition of bio-oils produced from low-mineral containing wood biomass. Yet, the wheat straw-derived bio-oil produced in the continuous unit comprised lower molecular weight compounds with fewer oxygen-containing functional groups and lower O/C and H/C ratios, compared to bio-oils from batch pyrolysis. Longer residence time of vapours in the heated zone in the rotary kiln and a higher mineral content in wheat straw resulted in increased catalytically-mediated secondary reactions that favoured further bio-oil decomposition. This work shows for the first time that it is possible to produce distinct bio-oils without the need for external catalyst addition, by matching reactor type/design and feedstock.
Improvement of a Nusselt-Based Simulation Model for Heat Transfer in Rotary Heat Exchangers
Authors: Melian Eloy , Klein Harald , Thißen Nikolaus
Published: December 2020

In the last 50 years, the technology of rotary heat exchangers has not changed considerably. A reliable simulation can help improve the design of this technology. In this work, a simulation for rotary heat exchangers was developed and validated with multiple experimental data. This simulation takes an innovative approach based on locally calculated heat transfer coefficients and considers the entry region effect. This approach proved to be accurate since the average difference between the experimental results and the proposed model with a constant heat boundary condition is 0.1% and the maximum absolute deviation 1%. Experimental, as well as simulation results, indicate that lower empty tube gas velocity (1 m/s) and higher rotational speed (12 rpm) improve thermal efficiency compared to commonly used operating conditions. Additionally, a new model for predicting the local internal Nusselt number for sine ducts in the rotor channels is proposed, which considers the entry region effect.
Integrated Transmission-and-Distribution System Modeling of Power Systems: State-of-the-Art and Future Research Directions
Authors: Jain Himanshu , Bhatti Bilal Ahmad , Wu Tianying , Mather Barry , Broadwater Robert
Published: December 2020

Integrated transmission-and-distribution (T&D) modeling is a new and developing method for simulating power systems. Interest in integrated T&D modeling is driven by the changes taking place in power systems worldwide that are resulting in more decentralized power systems with increasingly high levels of distributed energy resources. Additionally, the increasing role of the hitherto passive energy consumer in the management and operation of power systems requires more capable and detailed integrated T&D modeling to understand the interactions between T&D systems. Although integrated T&D modeling has not yet found widespread commercial application, its potential for changing the decades-old power system modeling approaches has led to several research efforts in the last few years that tried to (i) develop algorithms and software for steady-state and dynamic modeling of power systems and (ii) demonstrate the advantages of this modeling approach compared with traditional, separated T&D system modeling. In this paper, we provide a review of integrated T&D modeling research efforts and the methods employed for steady-state and dynamic modeling of power systems. We also discuss our current research in integrated T&D modeling and the potential directions for future research. This paper should be useful for power systems researchers and industry members because it will provide them with a critical summary of current research efforts and the potential topics where research efforts are needed to further advance and demonstrate the utility of integrated T&D modeling.
CSP Quasi-Dynamic Performance Model Development for All Project Life Cycle Stages and Considering Operation Modes. Validation Using One Year Data
Authors: Gonzalez Gonzalez Adrian , Alvarez Cabal J. Valeriano , Rodríguez Montequin Vicente , Villanueva Balsera Joaquín , Peón Menéndez Rogelio
Published: December 2020

The energy production of concentrated solar power (CSP) plants not only depends on their design, but also of the weather conditions and the way they are operated. A performance model (PM) of a CSP plant is an essential tool to determine production costs, to optimize design and also to supervise the operation of the plant. The challenge is developing a PM that is both easy enough to be useful during the earlier stages of the project, and also useful for supervision of plant operation. This requires one to be able to describe the step between the different modes of operation and to fit the response to transient meteorological phenomena, not so relevant in terms of aggregate values, but crucial for the supervision. The quasi-dynamic performance model (QD-PM) can predict the net energy exported to the grid, as well as all the key operational variables. The QD-PM was implemented using Matlab-Simulink of Mathwoks (MA, USA) with a modular structure. Each module is developed using specific software and a state machine is used to simulate the sequence between the operation modes. The validation of the PM is made using one complete year of commercial operation of a 50 MWe CSP plant situated in Spain. The comparison between the actual data and the results of the model shows an excellent fit, being especially noteworthy as follows the transients between the different CSP operation modes. Then, QD-PM provides an accuracy better than the usual PM, and, almost, as good as that of a fully dynamic model but with a shorter simulation time. But, the main advantage of the QD-PM is that it can be use not only in the feasibility and design stages, but it can be used to supervise the operation of the plant.
Adsorption Characteristics and Potential of Olive Cake Alkali Residues for Biodiesel Purification
Authors: Alnaief Mohammad , Sandouqa Arwa , Altarawneh Ibrahem , Al-Shannag Mohammad , Alkasrawi Malek , Al-hamamre Zayed
Published: December 2020

Mediterranean countries produce up to 97% of the total global olive production. Besides being a serious environmental burden, olive mill waste represents a potential resource of useful materials for recovery and valorization. In this work, the adsorption characteristics and potential of bioadsorbent material manufactured by alkali treatment of olive cake was evaluated. The resultant bioadsorbent was able to purify biodiesel to meets both ASTM D6751 and EN 14214 standards. Further, the sorption equilibrium was investigated at 25 °C. Langmuir, Freundlich, and Temkin isotherms fit to the experimental data were evaluated by applying the non-linear Chi-square test. Freundlich and Temkin isotherms were shown to adequately described the adsorption isotherms of the produced bioadsorbent.
The North Caucasus Region as a Blind Spot in the “European Green Deal”: Energy Supply Security and Energy Superpower Russia
Authors: Peña-Ramos José Antonio , Bagus Philipp , Amirov-Belova Dmitri
Published: December 2020

The “European Green Deal” has ambitious aims, such as net-zero greenhouse gas emissions by 2050. While the European Union aims to make its energies greener, Russia pursues power-goals based on its status as a geo-energy superpower. A successful “European Green Deal” would have the up-to-now underestimated geopolitical advantage of making the European Union less dependent on Russian hydrocarbons. In this article, we illustrate Russian power-politics and its geopolitical implications by analyzing the illustrative case of the North Caucasus, which has been traditionally a strategic region for Russia. The present article describes and analyses the impact of Russian intervention in the North Caucasian secessionist conflict since 1991 and its importance in terms of natural resources, especially hydrocarbons. The geopolitical power secured by Russia in the North Caucasian conflict has important implications for European Union’s energy supply security and could be regarded as a strong argument in favor of the “European Green Deal”.
Storage Technologies for the Electricity Transition: An Analysis of Actors, Actor Perspectives and Transition Pathways in Germany
Authors: Frey Ulrich J. , Wassermann Sandra , Deissenroth-Uhrig Marc
Published: December 2020

This article analyses actors in the storage niche during the German electricity transition. Thus, we develop a more differentiated understanding of actors and their storage activities. For that, we employ the analytical multi-level-perspective (MLP) framework to focus on interactions between old and new storage technologies. Using data from expert interviews, we investigate whether the storage pathway resembles any of the four ideal types of transition pathways for interactions between niche and regime. Through our interviews, we identify five types of actor in the storage market: Big 4 (EnBW, RWE, E.ON, Vattenfall), project developers, innovative municipal utilities, small rural municipal utilities and independent green electricity providers. For each actor, we analyse four main aspects (1) previous orientation and motivation, (2) structural strategies, (3) institutional strategies, and (4) product-related strategies. Parallel to the classification of actors, we also classify available storage technologies according to their primary field of application. We conclude that interactions between regime and niche actors are cooperative, but weak, and no specific actor type currently dominates the niche activities. Hence, applications in the storage niche are not yet ready for a larger market. In sum, our results point to a future system that is characterized by reconfiguration, not substitution or transformation of current market actors.
Sap Flow in Aleppo Pine in Greece in Relation to Sapwood Radial Gradient, Temporal and Climatic Variability
Authors: Korakaki Evangelia , Fotelli Mariangela N.
Published: December 2020

Research Highlights: The radial gradient of sap flux density (Js) and the effects of climatic factors on sap flow of Aleppo pine were assessed at different time scales in an eastern Mediterranean ecosystem to improve our understanding of the species water balance. Background and Objectives: Aleppo pine’s sap flow radial profile and responses to environmental parameters in the eastern Mediterranean were, to our best knowledge, originating to date from more arid planted forests. Information from natural forests in this region was lacking. Our objectives were to (a) determine the species’ radial variability in Js on a diurnal and seasonal basis and under different climatic conditions, (b) scale up to tree sap flow taking into account the radial profile of Js and (c) determine the responses of Aleppo pine’s sap flow over the year to climatic variability. Materials and Methods: Js was monitored in Aleppo pine in a natural forest in northern Greece with Granier’s method using sensors at three sapwood depths (21, 51, and 81 mm) during two periods differing in climatic conditions, particularly in soil water availability. Results: Js was the highest at 21 mm sapwood depth, and it declined with increasing depth. A steeper gradient of Js in deep sapwood was observed under drier conditions. The same patterns of radial variability in Js were maintained throughout the year, but the contribution of inner sapwood to sap flow was the highest in autumn when the lower seasonal Js was recorded in both study periods. Not taking into account the radial gradient of Js in the studied Aleppo pine would result in a c. 20.2–27.7 % overestimation of total sap flow on a sapwood basis (Qs), irrespective of climatic conditions. On a diurnal and seasonal basis, VPD was the strongest determinant of sap flux density, while at a larger temporal scale, the effect of soil water content was evident. At SWC > 20% sap flow responded positively to increasing solar radiation and VPD, indicating the decisive role of water availability in the studied region. Moreover, in drier days with VPD > 0.7 KPa, SWC controlled the variation of sap flow. Conclusions: There is a considerable radial variability in Js of the studied Aleppo pine and a considerable fluctuation of sap flow with environmental dynamics that should be taken into account when addressing the species water balance.
Molecular Properties of Flammulina velutipes Polysaccharide–Whey Protein Isolate (WPI) Complexes via Noncovalent Interactions
Authors: Shang Jiaqi , Liao Minhe , Jin Ritian , Teng Xiangyu , Li Hao , Xu Yan , Zhang Ligang , Liu Ning
Published: December 2020

Whey protein isolate (WPI) has a variety of nutritional benefits. The stability of WPI beverages has attracted a large amount of attention. In this study, Flammulina velutipes polysaccharides (FVPs) interacted with WPI to improve the stability via noncovalent interactions. Multiple light scattering studies showed that FVPs can improve the stability of WPI solutions, with results of radical scavenging activity assays demonstrating that the solutions of the complex had antioxidant activity. The addition of FVPs significantly altered the secondary structures of WPI, including its α-helix and random coil. The results of bio-layer interferometry (BLI) analysis indicated that FVPs interacted with the WPI, and the equilibrium dissociation constant (KD) was calculated as 1.736 × 10−4 M in this study. The in vitro digestibility studies showed that the FVPs protected WPI from pepsin digestion, increasing the satiety. Therefore, FVPs effectively interact with WPI through noncovalent interactions and improve the stability of WPI, with this method expected to be used in protein-enriched and functional beverages.
Preliminary Techno-Economic Assessment of Animal Cell-Based Meat
Authors: Risner Derrick , Li Fangzhou , Fell Jason S. , Pace Sara A. , Siegel Justin B. , Tagkopoulos Ilias , Spang Edward S.
Published: December 2020

Interest in animal cell-based meat (ACBM) or laboratory-grown meat has been increasing; however, the economic viability of these potential products has not been thoroughly vetted. Recent studies suggest monoclonal antibody production technology can be adapted for the industrialization of ACBM production. This study provides a scenario-based assessment of the projected cost per kilogram of ACBM produced in the United States based on cellular metabolic requirements and process/chemical engineering conventions. A sensitivity analysis of the model identified the nine most influential cost factors for ACBM production out of 67 initial parameters. The results indicate that technological performance will need to approach technical limits for ACBM to achieve profitably as a commodity. However, the model also suggests that low-volume high-value specialty products could be viable based on current technology.
Formulation of Heat-Induced Whey Protein Gels for Extrusion-Based 3D Printing
Authors: Sager Valeska F. , Munk Merete B. , Hansen Mikka Stenholdt , Bredie Wender L. P. , Ahrné Lilia
Published: December 2020

This study investigated the extrusion-based 3D printability of heat-induced whey protein gels as protein rich food inks. In particular, the effects of ionic strength by the addition of NaCl (0–250 mM), protein content (10%, 15%, 20%), fat content (0%, 10%), and partial substitution of whey protein isolate (WPI) with microparticulated whey protein (MWP) or micellar casein isolate (MCI) on printability were assessed. Texture analysis, specifically Young’s modulus, rheological measurements including yield stress, and creep–recovery behavior were used to characterize the gels. Modifications of the formulation in terms of ionic strength, increased protein content, and the formation of emulsion gels were insufficient to maintain a continuous extrusion process or shape stability after printing. However, the substitution of WPI with MWP created more viscoeleastic gels with improved printability and shape retention of the 3D cube structure after deposition. The partial replacement of WPI with MCI led to phase separation and 3D-printed cubes that collapsed after deposition. A narrow range of rheological material properties make WPI and MWP emulsion gels promising food inks for extrusion-based 3D printing.
Oxidative Stress, Glutathione Metabolism, and Liver Regeneration Pathways Are Activated in Hereditary Tyrosinemia Type 1 Mice upon Short-Term Nitisinone Discontinuation
Authors: Colemonts-Vroninks Haaike , Neuckermans Jessie , Marcelis Lionel , Claes Paul , Branson Steven , Casimir Georges , Goyens Philippe , Martens Geert A. , Vanhaecke Tamara , De Kock Joery
Published: December 2020

Hereditary tyrosinemia type 1 (HT1) is an inherited condition in which the body is unable to break down the amino acid tyrosine due to mutations in the fumarylacetoacetate hydrolase (FAH) gene, coding for the final enzyme of the tyrosine degradation pathway. As a consequence, HT1 patients accumulate toxic tyrosine derivatives causing severe liver damage. Since its introduction, the drug nitisinone (NTBC) has offered a life-saving treatment that inhibits the upstream enzyme 4-hydroxyphenylpyruvate dioxygenase (HPD), thereby preventing production of downstream toxic metabolites. However, HT1 patients under NTBC therapy remain unable to degrade tyrosine. To control the disease and side-effects of the drug, HT1 patients need to take NTBC as an adjunct to a lifelong tyrosine and phenylalanine restricted diet. As a consequence of this strict therapeutic regime, drug compliance issues can arise with significant influence on patient health. In this study, we investigated the molecular impact of short-term NTBC therapy discontinuation on liver tissue of Fah-deficient mice. We found that after seven days of NTBC withdrawal, molecular pathways related to oxidative stress, glutathione metabolism, and liver regeneration were mostly affected. More specifically, NRF2-mediated oxidative stress response and several toxicological gene classes related to reactive oxygen species metabolism were significantly modulated. We observed that the expression of several key glutathione metabolism related genes including Slc7a11 and Ggt1 was highly increased after short-term NTBC therapy deprivation. This stress response was associated with the transcriptional activation of several markers of liver progenitor cells including Atf3, Cyr61, Ddr1, Epcam, Elovl7, and Glis3, indicating a concreted activation of liver regeneration early after NTBC withdrawal.
The Shirom-Melamed Vigor Measure for Students: Factorial Analysis and Construct Validity in Spanish Undergraduate University Students
Authors: Pulido-Martos Manuel , Cortés-Denia Daniel , de la Rosa-Blanca Juan José , Lopez-Zafra Esther
Published: December 2020

Students suffer from a decrease in physical activity during their education period. This lower level of activity could affect, through various paths, their academic performance, mental health, and satisfaction with life. In these two studies, we assumed that vigor, a positive affect variable, would act as a mediating variable in the above relationship, and thus, we proposed an instrument for evaluating vigor in academic contexts. In Study 1, 707 undergraduates (59.7% women) responded to the vigor scale adapted for students to test factorial validation (through confirmatory factor analysis) and obtain reliability indicators. In Study 2, 309 undergraduates (55.3% women) completed a questionnaire measuring physical activity, mental health, satisfaction with life, vigor, and academic performance to test a structural model of the relationships between the variables to obtain construct validity. A measurement model with three related factors, each representing one dimension of vigor, optimally fit the data, and the reliability indices were adequate (Study 1). Moreover, the mediational model confirmed a complete influence of physical activity on satisfaction with life, academic performance, and mental health levels through students’ vigor levels with optimal adjusting values (Study 2). Proposing an instrument such as the Shirom-Melamed Vigor Measure for students allows the opening of a research venue that is focused on the study of positive affects in academic contexts, as well as the testing of the physical activity pathways of action in obtaining positive results.
Microplastics Pollution as an Invisible Potential Threat to Food Safety and Security, Policy Challenges and the Way Forward
Authors: Usman Sunusi , Abdull Razis Ahmad Faizal , Shaari Khozirah , Amal Mohammad Noor Azmai , Saad Mohd Zamri , Mat Isa Nurulfiza , Nazarudin Muhammad Farhan , Zulkifli Syaizwan Zahmir , Sutra Jumria , Ibrahim Musa Adamu
Published: December 2020

Technological advances, coupled with increasing demands by consumers, have led to a drastic increase in plastic production. After serving their purposes, these plastics reach our water bodies as their destination and become ingested by aquatic organisms. This ubiquitous phenomenon has exposed humans to microplastics mostly through the consumption of sea food. This has led the World Health Organization (WHO) to make an urgent call for the assessment of environmental pollution due to microplastics and its effect on human health. This review summarizes studies between 1999 and 2020 in relation to microplastics in aquatic ecosystems and human food products, their potential toxic effects as elicited in animal studies, and policies on their use and disposal. There is a paucity of information on the toxicity mechanisms of microplastics in animal studies, and despite their documented presence in food products, no policy has been in place so far, to monitor and regulates microplastics in commercial foods meant for human consumption. Although there are policies and regulations with respect to plastics, these are only in a few countries and in most instances are not fully implemented due to socioeconomic reasons, so they do not address the problem across the entire life cycle of plastics from production to disposal. More animal research to elucidate pathways and early biomarkers of microplastic toxicity that can easily be detected in humans is needed. This is to create awareness and influence policies that will address this neglected threat to food safety and security.
Initial COVID-19 Outbreak: An Epidemiological and Socioeconomic Case Review of Iran
Authors: Blandenier Elise , Habibi Zahra , Kousi Timokleia , Sestito Paolo , Flahault Antoine , Rozanova Liudmila
Published: December 2020

The coronavirus disease 2019 (COVID-19) pandemic has affected millions of people worldwide. It brought about the implementation of various measures and restrictions at a global level. Iran has been one of the countries with the highest rates of COVID-19 cases. This study reviews the initial outbreak of COVID-19 in Iran and examines the mitigation strategies adopted by the country. Moreover, it reports the socioeconomic challenges faced by the authorities during the efforts to contain the virus. A transdisciplinary literature review was carried out and a political measures timeline was constructed. A broad overview of the initial phase of the COVID-19 outbreak in Iran is presented, starting from the first confirmed case on 19 February 2020 until April 2020. The results of this epidemiological and socioeconomic case review of Iran suggests that the political measures undertaken by the Republic of Iran contributed to the decrease of the prevalence of COVID-19. However, due to the existing financial bottleneck, Iran has faced limited health system resources. Therefore, the response was not sufficient to restrict the spread and the efficient handling of the virus in the long-term.
The Relationship between the Sense of Coherence of Dental Hygiene Students in Their Graduation Year and Their View of the Profession and Attitude to Work: A Cross-Sectional Survey in Japan
Authors: Tano Rumi , Miura Hiroko , Oshima Katsuo , Noritake Kanako , Fukuda Hideki
Published: December 2020

Objective: The need to make sense of coherence in placement support for student dental hygienists has been shown. On this basis, this study investigated the relationship between the sense of coherence of student dental hygienists and their view of the profession and attitude to work in order to clarify how they perceive their prospects for employment. Methods: The subjects were graduation-year students at all of the dental hygienist training institutions in Japan, and anonymous, self-administered questionnaires were sent to the institutions by post in 2019. The results were analyzed by χ2 tests, as well as one-way analysis of variance and multiple comparisons using Tukey’s test, with the level of significance set at 5%. Results: Of 6270 questionnaires that were returned, 6264 were analyzed. The sense of coherence (SOC) component senses were manageability (F(26,221) = 5306.06, p < 0.01), meaningfulness (F(26,222) = 4373.48, p < 0.01), and comprehensibility (F(26,216) = 3986.12, p < 0.01), with meaningfulness scoring significantly higher than the other two (p < 0.01). Analysis with SOC scores divided into the low, medium, and high groups showed a relationship between the SOC of student dental hygienists and their view of the profession and attitude to work (p < 0.01), such that higher SOC scores were associated with a better view of the profession and a better attitude to work (F(26,225) = 282.18, p < 0.01). Conclusions: The results suggest that education that increases SOC in dental hygienist training programs may positively affect future prospects for student dental hygienists.
Entrepreneurs’ Capacity for Mentalizing: Its Influence on Burnout Syndrome
Authors: Manzano-García Guadalupe , Ayala-Calvo Juan Carlos , Desrumaux Pascale
Published: December 2020

Burnout is a mental disorder that leads to difficulties for the entrepreneur in controlling his or her personal and professional life. The most common consequences of entrepreneurial burnout include the subject experiencing low motivation, low organizational commitment, loss of energy, demoralization in connection with their work, poor quality of work, feeling of failure, and the perception that his or her company is performing poorly. We used a sample of 157 Spanish entrepreneurs selected at random from the Iberian Balance Sheet Analysis System database. We employed the Spanish version of the Reflective Functioning Questionnaire to measure mentalizing and the Spanish version of the Maslach-Burnout Inventory-General Survey (MBI-GS) to measure burnout. This research showed that entrepreneurial burnout could be avoided in part if the entrepreneur achieved a good capacity for mentalizing. Hypomentalizing contributed to explaining entrepreneurs’ levels of professional efficacy, cynicism, and emotional exhaustion. In contrast, the explanatory power of hypermentalizing was not significant for any of the dimensions of burnout. This study provides new evidence of burnout in entrepreneurs; a professional group with an important economic, politic, and social role has been little studied.