bluuebunny/crossref_metadata_2025 huggingface dataset:
Synergistic antitumor activity by dual blockade of CCR1 and CXCR2 expressed on myeloid cells within the tumor microenvironment
Authors: Masui Hideyuki , Kawada Kenji , Itatani Yoshiro , Hirai Hideyo , Nakanishi Yuki , Kiyasu Yoshiyuki , Hanada Keita , Okamoto Michio , Hirata Wataru , Nishikawa Yasuyo , Sugimoto Naoko , Tamura Takuya , Sakai Yoshiharu , Obama Kazutaka
Published: May 2024

Background

Chemokine signaling within the tumor microenvironment can promote tumor progression. Although CCR1 and CXCR2 on myeloid cells could be involved in tumor progression, it remains elusive what effect would be observed if both of those are blocked.

Methods

We employed two syngeneic colorectal cancer mouse models: a transplanted tumor model and a liver metastasis model. We generated double-knockout mice for CCR1 and CXCR2, and performed bone marrow (BM) transfer experiments in which sub-lethally irradiated wild-type mice were reconstituted with BM from either wild-type, Ccr1−/−, Cxcr2−/− or Ccr1−/−Cxcr2−/− mice.

Results

Myeloid cells that express MMP2, MMP9 and VEGF were accumulated around both types of tumors through CCR1- and CXCR2-mediated pathways. Mice reconstituted with Ccr1−/−Cxcr2−/− BM exhibited the strongest suppression of tumor growth and liver metastasis compared with other three groups. Depletion of CCR1+CXCR2+ myeloid cells led to a higher frequency of CD8+ T cells, whereas the numbers of Ly6G+ neutrophils, FOXP3+ Treg cells and CD31+ endothelial cells were significantly decreased. Furthermore, treatment with a neutralizing anti-CCR1 mAb to mice reconstituted with Cxcr2−/− BM significantly suppressed tumor growth and liver metastasis.

Conclusion

Dual blockade of CCR1 and CXCR2 pathways in myeloid cells could be an effective therapy against colorectal cancer.
Comparison of intramyocellular lipid metabolism in patients with diabetes and male athletes
Authors: Mezincescu Alice M. , Rudd Amelia , Cheyne Lesley , Horgan Graham , Philip Sam , Cameron Donnie , van Loon Luc , Whitfield Phil , Gribbin Rachael , Hu May Khei , Delibegovic Mirela , Fielding Barbara , Lobley Gerald , Thies Frank , Newby David E. , Gray Stuart , Henning Anke , Dawson Dana
Published: May 2024

Despite opposing insulin sensitivity and cardiometabolic risk, both athletes and patients with type 2 diabetes have increased skeletal myocyte fat storage: the so-called “athlete’s paradox”. In a parallel non-randomised, non-blinded trial (NCT03065140), we characterised and compared the skeletal myocyte lipid signature of 29 male endurance athletes and 30 patients with diabetes after undergoing deconditioning or endurance training respectively. The primary outcomes were to assess intramyocellular lipid storage of the vastus lateralis in both cohorts and the secondary outcomes were to examine saturated and unsaturated intramyocellular lipid pool turnover. We show that athletes have higher intramyocellular fat saturation with very high palmitate kinetics, which is attenuated by deconditioning. In contrast, type 2 diabetes patients have higher unsaturated intramyocellular fat and blunted palmitate and linoleate kinetics but after endurance training, all were realigned with those of deconditioned athletes. Improved basal insulin sensitivity was further associated with better serum cholesterol/triglycerides, glycaemic control, physical performance, enhanced post insulin receptor pathway signalling and metabolic sensing. We conclude that insulin-resistant, maladapted intramyocellular lipid storage and turnover in patients with type 2 diabetes show reversibility after endurance training through increased contributions of the saturated intramyocellular fatty acid pools. Clinical Trial Registration: NCT03065140: Muscle Fat Compartments and Turnover as Determinant of Insulin Sensitivity (MISTY)
Cryo-EM structures of the human Elongator complex at work
Authors: Abbassi Nour-el-Hana , Jaciuk Marcin , Scherf David , Böhnert Pauline , Rau Alexander , Hammermeister Alexander , Rawski Michał , Indyka Paulina , Wazny Grzegorz , Chramiec-Głąbik Andrzej , Dobosz Dominika , Skupien-Rabian Bozena , Jankowska Urszula , Rappsilber Juri , Schaffrath Raffael , Lin Ting-Yu , Glatt Sebastian
Published: May 2024

tRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm5U34) in eukaryotic tRNAs. However, the structure and function of human Elongator remain poorly understood. In this study, we present a series of cryo-EM structures of human ELP123 in complex with tRNA and cofactors at four different stages of the reaction. The structures at resolutions of up to 2.9 Å together with complementary functional analyses reveal the molecular mechanism of the modification reaction. Our results show that tRNA binding exposes a universally conserved uridine at position 33 (U33), which triggers acetyl-CoA hydrolysis. We identify a series of conserved residues that are crucial for the radical-based acetylation of U34 and profile the molecular effects of patient-derived mutations. Together, we provide the high-resolution view of human Elongator and reveal its detailed mechanism of action.
Switchable supramolecular helices for asymmetric stereodivergent catalysis
Authors: Chen Ran , Hammoud Ahmad , Aoun Paméla , Martínez-Aguirre Mayte A. , Vanthuyne Nicolas , Maruchenko Régina , Brocorens Patrick , Bouteiller Laurent , Raynal Matthieu
Published: May 2024

Despite recent developments on the design of dynamic catalysts, none of them have been exploited for the in-situ control of multiple stereogenic centers in a single molecular scaffold. We report herein that it is possible to obtain in majority any amongst the four possible stereoisomers of an amino alcohol by means of a switchable asymmetric catalyst built on supramolecular helices. Hydrogen-bonded assemblies between a benzene-1,3,5-tricarboxamide (BTA) achiral phosphine ligand coordinated to copper and a chiral BTA comonomer are engaged in a copper-hydride catalyzed hydrosilylation and hydroamination cascade process. The nature of the product stereoisomer is related to the handedness of the helices and can thus be directed in a predictable way by changing the nature of the major enantiomer of the BTA comonomer present in the assemblies. The strategy allows all stereoisomers to be obtained one-pot with similar selectivities by conducting the cascade reaction in a concomitant manner, i.e. without inverting the handedness of the helices, or sequentially, i.e. by switching the handedness of the supramolecular helices between the hydrosilylation and hydroamination steps. Supramolecular helical catalysts appear as a unique and versatile platform to control the configuration of molecules or polymers embedding several stereogenic centers.
Pick-up and assembling of chemically sensitive van der Waals heterostructures using dry cryogenic exfoliation
Authors: Patil Vilas , Ghosh Sanat , Basu Amit , Kuldeep  , Dutta Achintya , Agrawal Khushabu , Bhatia Neha , Shah Amit , Jangade Digambar A. , Kulkarni Ruta , Thamizhavel A. , Deshmukh Mandar M.
Published: May 2024

Assembling atomic layers of van der Waals materials (vdW) combines the physics of two materials, offering opportunities for novel functional devices. Realization of this has been possible because of advancements in nanofabrication processes which often involve chemical processing of the materials under study; this can be detrimental to device performance. To address this issue, we have developed a modified micro-manipulator setup for cryogenic exfoliation, pick up, and transfer of vdW materials to assemble heterostructures. We use the glass transition of a polymer PDMS to cleave a flake into two, followed by its pick-up and drop to form pristine twisted junctions. To demonstrate the potential of the technique, we fabricated twisted heterostructure of Bi2Sr2CaCu2O8+x (BSCCO), a van der Waals high-temperature cuprate superconductor. We also employed this method to re-exfoliate NbSe2 and make twisted heterostructure. Transport measurements of the fabricated devices indicate the high quality of the artificial twisted interface. In addition, we extend this cryogenic exfoliation method for other vdW materials, offering an effective way of assembling heterostructures and twisted junctions with pristine interfaces.
Courtyard geometry’s effect on energy consumption of AlKharga city residential buildings, Egypt
Authors: Seddik Hassan Ahmed M. , Abd El Aal Reham Fathy Ahmed , Fahmi Asmaa Abd elmoneim , Ali Sherif Mohamed Ahmed , Abdelhady Mohamed I. M.
Published: May 2024

The research aim is to clarify the effect of courtyard placement, the ratio between length and width, and courtyard orientation on energy consumption in residential buildings in hot and dry climates, to seek planning and designing alternatives for new cities and new residential complexes that are consistent with the environment and climate and save the consumption of energy used in the buildings. The research method was conducted through Design Builder software for simulation purposes. The reference model with the 157.25 m2 which accommodates a central square courtyard measuring 5 m × 5 m, on a residential building model in the New Valley Governorate of Kharga City, Egypt. The courtyard simulation is aimed to determine Less energy consumption inside the different case studies, in 9 courtyard placements The studied alternatives for Courtyard Placement, are (the center of the building, sub facades, and main facades). The different ratios are (1:1, 1.25:1, 1.5:1–1.75:1, 2:1, 2.25:1, 2.5–1). The longitudinal axis of the Courtyard has been oriented to the east–west direction for all placements, and north–south direction for all placements. Also, (orientation angle is Zero), it offered the percentages of better and worst cases in each position of the courtyard. The research findings suggest that the best Placement of the Courtyard that achieved the highest rate of saving of energy consumed inside the used building model was at the southwestern façade, with a saving rate of 18.73%. Then, the Placement of the Courtyard at the northwestern and southeastern facades with a saving rate of 17.91%, with a length-to-width ratio (2.5:1) if the longitudinal axis of the Courtyard is oriented in the north–south direction, Through the study, we conclude that the placement and orientation of the courtyard and its regular formation have contributed to rationalizing energy consumption in residential buildings, the study reached some important standards that could represent a methodological framework for designing contemporary residential buildings with an energy-efficient inner courtyard.
An adaptive prosthetic socket for people with transtibial amputation
Authors: Sanders Joan E. , Vamos Andrew C. , Mertens Joseph C. , Allyn Katheryn J. , Larsen Brian G. , Ballesteros Daniel , Wang Horace , DeGrasse Nicholas S. , Garbini Joseph L. , Hafner Brian J. , Friedly Janna L.
Published: May 2024

It is essential that people with limb amputation maintain proper prosthetic socket fit to prevent injury. Monitoring and adjusting socket fit, for example by removing the prosthesis to add prosthetic socks, is burdensome and can adversely affect users’ function and quality-of-life. This study presents results from take-home testing of a motor-driven adaptive socket that automatically adjusted socket size during walking. A socket fit metric was calculated from inductive sensor measurements of the distance between the elastomeric liner surrounding the residual limb and the socket’s inner surface. A proportional-integral controller was implemented to adjust socket size. When tested on 12 participants with transtibial amputation, the controller was active a mean of 68% of the walking time. In general, participants who walked more than 20 min/day demonstrated greater activity, less doff time, and fewer manual socket size adjustments for the adaptive socket compared with a locked non-adjustable socket and a motor-driven socket that participants adjusted with a smartphone application. Nine of 12 participants reported that they would use a motor-driven adjustable socket if it were available as it would limit their socket fit issues. The size and weight of the adaptive socket were considered the most important variables to improve.
An optoelectronic implantable neurostimulation platform allowing full MRI safety and optical sensing and communication
Authors: Doguet Pascal , Garnier Jérôme , Nieuwenhuys Aurore , Godfraind Carmen , Botquin Yohan , Lemaire Antoine , Justice John , Nonclercq Antoine , El Tahry Riëm , Corbett Brian , Delbeke Jean
Published: May 2024

A novel programmable implantable neurostimulation platform based on photonic power transfer has been developed for various clinical applications with the main focus of being safe to use with MRI scanners. The wires usually conveying electrical current from the neurostimulator to the electrodes are replaced by optical fibers. Photovoltaic cells at the tip of the fibers convert laser light to biphasic electrical impulses together with feedback signals with 54% efficiency. Furthermore, a biocompatible, implantable and ultra-flexible optical lead was developed including custom optical fibers. The neurostimulator platform incorporates advanced signal processing and optical physiological sensing capabilities thanks to a hermetically sealed transparent nonmetallic casing. Skin transparency also allowed the development of a high-speed optical transcutaneous communication channel. This implantable neurostimulation platform was first adapted to a vagus nerve stimulator for the treatment of epilepsy. This neurostimulator has been designed to fulfill the requirements of a class III long-term implantable medical device. It has been proven compliant with standard ISO/TS10974 for 1.5 T and 3 T MRI scanners. The device poses no related threat and patients can safely undergo MRI without specific or additional precautions. Especially, the RF induced heating near the implant remains below 2 °C whatever the MRI settings used. The main features of this unique advanced neurostimulator and its architecture are presented. Its functional performance is evaluated, and results are described with a focus on optoelectronics aspects and MRI safety.
Biodegradable plastic formulated from chitosan of Aristeus antennatus shells with castor oil as a plasticizer agent and starch as a filling substrate
Authors: El Feky Ayaat R. , Ismaiel Mohammed , Yılmaz Murat , Madkour Fedekar M. , El Nemr Ahmed , Ibrahim Hassan A. H.
Published: May 2024

Biodegradable plastics are those subjected easily to a degradation process, in which they can be decomposed after disposal in the environment through microbial activity. 30 bioplastic film formulations based only on chitosan film were used in the current investigation as a positive control together with chitosan film recovered from chitin-waste of locally obtained Aristeus antennatus. Additionally, castor oil was used as a plasticizer. While the yield of chitosan was 18% with 7.65% moisture content and 32.27% ash in the shells, the isolated chitin had a degree of deacetylation (DD) of 86%. The synthesized bioplastic films were characterized via numerous criteria. Firstly, the swelling capacity of these biofilms recorded relatively high percentages compared to polypropylene as synthetic plastic. Noticeably, the FTIR profiles, besides DSC, TGA, and XRD, confirmed the acceptable characteristics of these biofilms. In addition, their SEM illustrated the homogeneity and continuity with a few straps of the chitosan film and showed the homogeneous mixes of chitosan and castor oil with 5 and 20%. Moreover, data detected the antibacterial activity of different bioplastic formulas against some common bacterial pathogens (Enterococcus feacalis, Kelbsiella pnumina, Bacillus subtilis, and Pseudomonas aeruginosa). Amazingly, our bioplastic films have conducted potent antimicrobial activities. So, they may be promising in such a direction. Further, the biodegradability efficacy of bioplastic films formed was proved in numerous environments for several weeks of incubation. However, all bioplastic films decreased in their weights and changed in their colors, while polypropylene, was very constant all the time. The current findings suggest that our biofilms may be promising for many applications, especially in the field of food package protecting the food, and preventing microbial contamination, consequently, it may help in extending the shelf life of products.
Persistence of viral RNA in North American elk experimentally infected with an ancestral strain of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Authors: Boggiatto Paola M. , Buckley Alexandra , Cassmann Eric D. , Seger Hannah , Olsen Steven C. , Palmer Mitchell V.
Published: May 2024

White-tailed deer (Odocoileus virginianus) have emerged as a reservoir host for SARS-CoV-2 given their susceptibility to infection and demonstrated high rates of seroprevalence and infection across the United States. As SARS-CoV-2 circulates within free-ranging white-tailed deer populations, there is the risk of transmission to other wildlife species and even back to the human population. The goal of this study was to determine the susceptibility, shedding, and immune response of North American elk (Cervus elaphus canadensis) to experimental infection with SARS-CoV-2, to determine if another wide-ranging cervid species could potentially serve as a reservoir host for the virus. Here we demonstrate that while North American elk do not develop clinical signs of disease, they do develop a neutralizing antibody response to infection, suggesting the virus is capable of replicating in this mammalian host. Additionally, we demonstrate SARS-CoV-2 RNA presence in the medial retropharyngeal lymph nodes of infected elk three weeks after experimental infection. Consistent with previous observations in humans, these data may highlight a mechanism of viral persistence for SARS-CoV-2 in elk.
Cholesterol accumulation impairs HIF-1α-dependent immunometabolic reprogramming of LPS-stimulated macrophages by upregulating the NRF2 pathway
Authors: Ting Kenneth K. Y. , Yu Pei , Dow Riley , Ibrahim Hisham , Karim Saraf , Polenz Chanele K. , Winer Daniel A. , Woo Minna , Jongstra-Bilen Jenny , Cybulsky Myron I.
Published: May 2024

Lipid accumulation in macrophages (Mφs) is a hallmark of atherosclerosis. Yet, how lipid loading modulates Mφ inflammatory responses remains unclear. We endeavored to gain mechanistic insights into how pre-loading with free cholesterol modulates Mφ metabolism upon LPS-induced TLR4 signaling. We found that activities of prolyl hydroxylases (PHDs) and factor inhibiting HIF (FIH) are higher in cholesterol loaded Mφs post-LPS stimulation, resulting in impaired HIF-1α stability, transactivation capacity and glycolysis. In RAW264.7 cells expressing mutated HIF-1α proteins resistant to PHDs and FIH activities, cholesterol loading failed to suppress HIF-1α function. Cholesterol accumulation induced oxidative stress that enhanced NRF2 protein stability and triggered a NRF2-mediated antioxidative response prior to and in conjunction with LPS stimulation. LPS stimulation increased NRF2 mRNA and protein expression, but it did not enhance NRF2 protein stability further. NRF2 deficiency in Mφs alleviated the inhibitory effects of cholesterol loading on HIF-1α function. Mutated KEAP1 proteins defective in redox sensing expressed in RAW264.7 cells partially reversed the effects of cholesterol loading on NRF2 activation. Collectively, we showed that cholesterol accumulation in Mφs induces oxidative stress and NRF2 stabilization, which when combined with LPS-induced NRF2 expression leads to enhanced NRF2-mediated transcription that ultimately impairs HIF-1α-dependent glycolytic and inflammatory responses.
Bio-based protic salts as precursors for sustainable free-standing film electrodes
Authors: Brzęczek-Szafran Alina , Gwóźdź Magdalena , Gaida Bartłomiej , Krzywiecki Maciej , Pawlyta Mirosława , Blacha-Grzechnik Agata , Kolanowska Anna , Chrobok Anna , Janas Dawid
Published: May 2024

Transforming amines with low boiling points and high volatilities into protic salts is a versatile strategy to utilize low molecular weight compounds as precursors for N-doped carbon structures in a straightforward carbonization procedure. Herein, conventional mineral acids commonly used for the synthesis of protic salts were replaced by bio-derived phytic acid, which, combined with various amines and amino acids, yielded partially or fully bio-derived protic salts. The biomass-based salts showed higher char-forming ability than their mineral acid-based analogs (up to 55.9% at 800°), simultaneously providing carbon materials with significant porosity (up to 1177 m2g−1) and a considerable level of N,P,O-doping. Here, we present the first comprehensive study on the correlation between the structure of the bio-derived protic precursors and the properties of derived carbon materials to guide future designs of biomass-derived precursors for the one-step synthesis of sustainable carbon materials. Additionally, we demonstrate how to improve the textural properties of the protic-salt-derived carbons (which suffer from high brittleness) by simply upgrading them into highly flexible nanocomposites using high-quality single-walled carbon nanotubes. Consequently, self-standing electrodes for the oxygen reduction reaction were created.
Comparison of the vertical accuracy of satellite-based correction service and the PPK GNSS method for obtaining sensor positions on a multibeam bathymetric survey
Authors: de Andrade Neto Walmor Pereira , Paz Igor da Silva Rocha , Oliveira Raquel Aparecida Abrahão Costa e , De Paulo Maurício Carvalho Mathias
Published: May 2024

The MarineStar Global Navigation Satellite System (GNSS) augmentation services are widely used in bathymetric surveys to obtain the sensor position. The postprocessed kinematic (PPK) GNSS method can be used to obtain more accurate positions. Therefore, the present work aims to verify the vertical final accuracies and uncertainties of exclusive order multibeam hydrographic surveys using the Global Navigation Satellite System (GNSS) as a horizontal positioning tool through methods such MarineStar services and PPK relative positioning, with comparisons between the two methods based on the results of the hydrographic base created. We produced two surfaces with each method, representing the minimum and maximum depths of the bathymetry point cloud. A statistical analysis of the differences between the depth surfaces obtained with the two methods was carried out. When comparing the two surfaces using the PPK method, the difference was less than 10 cm at 95.1% of the points. When comparing the two surfaces to those of the MarineStar, the difference between the maximum and minimum depths was less than 10 cm at 93.1% of the points. The results suggest that using the same survey data, the products obtained with the PPK method would comply with the hydrographic standards, while the MarineStar products would not.
Modified correlated measurement errors model for estimation of population mean utilizing auxiliary information
Authors: Singh Housila P. , Garg Neha
Published: May 2024

The existence of measurement errors cannot be avoided in practice. It is a prominent fact that the existence of measurement errors diminishes conventional properties of the estimators. A modified correlated measurement errors model has been proposed. Shalabh and Tsai (Commun Stat Simul Comput 46(7):5566–5593. 10.1080/03610918.2016.1165845, 2017) correlated measurement errors model is a particular member of the suggested modified model. In this article, we have tackled the estimation of population mean utilizing auxiliary information under modified correlated measurement errors model. We have developed ratio and product estimators and studied their properties in case of simple random sampling without replacement (SRSWOR) up to first order of approximation. It has been illustrated that suggested ratio and product estimators are more efficient than the conventional unbiased estimator as well as Shalabh and Tsai (Commun Stat Simul Comput 46(7):5566–5593. 10.1080/03610918.2016.1165845, 2017) ratio and product estimators under very realistic situations. An empirical study has also been performed to demonstrate the merits of the recommended estimators over other estimators.
Cardiac diastolic maladaptation is associated with the severity of exercise intolerance in sickle cell anemia patients
Authors: d’Humières Thomas , Bouvarel Antoine , Boyer Laurent , Savale Laurent , Guillet Henri , Alassaad Lara , de Luna Gonzalo , Berti Enora , Iles Sihem , Pham Hung d’Alexandry d’Orengiani Anne Laure , Audureau Etienne , Troupe Marie-Joelle , Schlatter Reine-Claude , Lamadieu Anaïs , Galactéros Frédéric , Derumeaux Geneviève , Messonnier Laurent A. , Bartolucci Pablo
Published: May 2024

This pilot study focusing on Sickle Cell Anemia (SCA) patients offers a comprehensive and integrative evaluation of respiratory, cardiovascular, hemodynamic, and metabolic variables during exercise. Knowing that diastolic dysfunction is frequent in this population, we hypothesize that a lack of cardiac adaptation through exercise might lead to premature increase in blood lactate concentrations in SCA patients, a potential trigger for acute disease complication. SCA patients were prospectively included in PHYSIO-EXDRE study and underwent a comprehensive stress test with a standardized incremental exercise protocol up to 4 mmol L−1blood lactate concentration (BL4). Gas exchange, capillary lactate concentration and echocardiography were performed at baseline, during stress test (at ∼ 2 mmol L−1) and BL4. The population was divided into two groups and compared according to the median value of percentage of theoretical peak oxygen uptake (%$${\dot{\text{V}}\text{O}}_{2peakth}$$V˙O2peakth) at BL4. Twenty-nine patients were included (42 ± 12 years old, 48% of women). Most patients reached BL4 at low-intensity exercise [median value of predicted power output (W) was 37%], which corresponds to daily life activities. The median value of %$${\dot{\text{V}}\text{O}}_{2peakth}$$V˙O2peakthat BL4 was 39%. Interestingly, diastolic maladaptation using echocardiography during stress test along with hemoglobin concentration were independently associated to early occurrence of BL4. As BL4 occurs for low-intensity exercises, SCA patients may be subject to acidosis-related complications even during their daily life activities. Beyond assessing physical capacities, our study underlines that diastolic maladaptation during exercise is associated with an early increase in blood lactate concentration.
A distributed geometric rewiring model
Authors: Lopez-Chavira Magali Alexander , Aguirre-Guerrero Daniela , Marcelín-Jiménez Ricardo , Vásquez-Toledo Luis Alberto , Bernal-Jaquez Roberto
Published: May 2024

We propose a distributed rewiring model which starts with a planar graph embedded into the Euclidean space and then behaves as a distributed system, where each node is provided with a set of dynamic links. The proposed rewiring evolves through cycles, where nodes explore the network to identify possible shortcuts and rewire their dynamic links. The rewiring decisions are subject to Euclidean and geodesic distance constrains. The emerging networks were assessed through topological and robustness analyses. We found that the networks display a variety of characteristics observed in complex networks encompassing phenomena such as preferential attachment, the distinctive traits of small-world networks, the presence of community structures, and robustness against degradation process. We consider that our proposal can be applied in the design of those self-managed systems in which there is a limitation on communication resources that can be represented by the Euclidean distance and, however, the components themselves can deploy strategies to optimize the transport of information and develop tolerance before contingencies.
Correlative geochemical imaging of Desmophyllum dianthus reveals biomineralisation strategy as a key coral vital effect
Authors: Standish Christopher D. , Trend Jacob , Kleboe Jacob , Chalk Thomas B. , Mahajan Sumeet , Milton J. Andy , Page Tessa M. , Robinson Laura F. , Stewart Joseph A. , Foster Gavin L.
Published: May 2024

The chemical and isotopic composition of stony coral skeletons form an important archive of past climate. However, these reconstructions are largely based on empirical relationships often complicated by “vital effects” arising from uncertain physiological processes of the coral holobiont. The skeletons of deep-sea corals, such as Desmophyllum dianthus, are characterised by micron-scale or larger geochemical heterogeneity associated with: (1) centres of calcification (COCs) where nucleation of new skeleton begins, and (2) fibres that thicken the skeleton. These features are difficult to sample cleanly using traditional techniques, resulting in uncertainty surrounding both the causes of geochemical differences and their influence on environmental signals. Here we combine optical, and in-situ chemical and isotopic, imaging tools across a range of spatial resolutions (~ 100 nm to 10 s of μm) in a correlative multimodal imaging (CMI) approach to isolate the microstructural geochemistry of each component. This reveals COCs are characterised by higher organic content, Mg, Li and Sr and lower U, B and δ11B compared to fibres, reflecting the contrasting biomineralisation mechanisms employed to construct each feature. CMI is rarely applied in Environmental/Earth Sciences, but here we illustrate the power of this approach to unpick the “vital effects” in D. dianthus, and by extension, other scleractinian corals.
Effect of common horsetail extract on growth characteristics, essential oil yield and chemical compositions of basil (Ocimum basilicum L.)
Authors: Eghlima Ghasem , Chegini Khodabakhsh Goodarzvand , Farzaneh Mohsen , Aghamir Fateme
Published: May 2024

To investigate the effect of horsetail extract containing high silicon on morphological traits, growth, content, and compositions of essential oil of sweet basil (Ocimum basilicum L.) an experiment turned into carried out in the shape of a randomized complete block design with three replications. Foliar treatment of horsetail extract with zero, 0.5, 1, and 2% concentrations was applied on 6–8 leaf plants. The assessed traits include plant height, number of leaves per plant, number of sub-branches, leaf area index, plant fresh weight, plant dry weight, total anthocyanin, the content of total phenol and total flavonoid, antioxidant activity, essential oil content, and compounds were measured. The findings demonstrated that the increase of silicon-containing horsetail extract enhanced the improved increase in growth and phytochemical trait values. The use of horsetail extract in the 2% treatment increased plant height, the number of leaves per plant, the number of sub-branches, leaf area index, fresh weight, and dry weight of the plant by 49.79, 45.61, 91.09, 99.78, 52.78 and 109.25%, respectively, compared to the control. The highest content of total phenol (2.12 mg GAE/g DW), total flavonoid (1.73 mg RE/g DW), total anthocyanin (0.83 mg C3G/g DW), and antioxidant activity (184.3 µg/ml) was observed in the 2% extract treatment. The content of essential oil increased with increasing the concentration of horsetail extract, so the highest amount of essential oil was obtained at the concentration of 2%, which increased by 134.78% compared to the control. By using GC–MS, the essential oil was analyzed. The main components of the essential oil include methyl eugenol (12.93–25.93%), eugenol (17.63–27.51%), 1,8-cineole (15.63–20.84%), linalool (8.31–19.63%) and (Z)-caryophyllene (6.02–14.93%). Increasing the concentration of horsetail extract increased the compounds of eugenol, 1,8-cineole, and linalool in essential oil compared to the control, but decreased the compounds of methyl eugenol and (Z)-caryophyllene. Foliar spraying of horsetail extract, which contains high amounts of silicon, as a stimulant and biological fertilizer, can be a beneficial ingredient in increasing the yield and production of medicinal plants, especially in organic essential oil production.
Prevalence of free flap failure in mandibular osteoradionecrosis reconstruction: a systematic review and meta-analysis
Authors: Kostares Evangelos , Kostares Michael , Kostare Georgia , Tsakris Athanasios , Kantzanou Maria
Published: May 2024

Our study aimed to estimate the prevalence of total free flap failure following free flap reconstruction for mandibular osteoradionecrosis (mORN) and assess the impact of potential moderators on this outcome. A comprehensive systematic literature search was independently conducted by two reviewers using the Medline, Scopus, Web of Science and Cochrane Library databases. Quality assessment of the selected studies was performed, and prevalence estimates with 95% confidence intervals (CI) were calculated. Outlier and influential analyses were conducted, and meta-regression analyses was employed to investigate the effects of continuous variables on the estimated prevalence. Ultimately, forty-six eligible studies (involving 1292 participants and 1344 free flaps) were included in our meta-analysis. The findings of our study revealed a prevalence of 3.1% (95% CI 1.3–5.4%) for total free flap failure after reconstruction for mORN. No study was identified as critically influential, and meta-regression analysis did not pinpoint any potential sources of heterogeneity. These findings provide valuable insights for researchers and serve as a foundation for future investigations into the management of mandibular osteoradionecrosis and the prevention of free flap failure in this context.
Alternated activation with relaxation of periosteum stimulates bone modeling and remodeling
Authors: Saulacic Nikola , Katagiri Hiroki , Fujioka-Kobayashi Masako , Ferrari Serge L. , Gerbaix Maude C.
Published: May 2024

Gradual elevation of the periosteum from the original bone surface, based on the principle of distraction osteogenesis, induces endogenous hard and soft tissue formation. This study aimed to assess the impact of alternating protocols of activation with relaxation (periosteal pumping) on bone modeling and remodeling. One hundred and sixty-two adult male Wistar rats were used in this study. Four test groups with different pumping protocols were created based on the relaxation applied. Two control groups underwent an activation period without relaxation or only a single activation. One group was sham-operated. Periosteal pumping without period of activation induced gene expression in bone and bone remodeling, and following activation period enhanced bone modeling. Four test groups and control group with activation period equaled the values of bone modeling at the end-consolidation period, showing significant downregulation of Sost in the bone and periosteum compared to that in the sham group (p < 0.001 and p < 0.001, respectively). When all test groups were pooled together, plate elevation from the bony surface increased bone remodeling on day 45 of the observation period (p = 0.003). Furthermore, bone modeling was significantly affected by plate elevation on days 17 and 45 (p = 0.047 and p = 0.005, respectively) and by pumping protocol on day 31 (p = 0.042). Periosteal pumping was beneficial for increasing bone repair when the periosteum remained in contact with the underlaying bony surface during the manipulation period. Following periosteal elevation, periosteal pumping accelerated bone formation from the bony surface by the modeling process.
Decoding predicted musical notes from omitted stimulus potentials
Authors: Ishida Kai , Ishida Tomomi , Nittono Hiroshi
Published: May 2024

Electrophysiological studies have investigated predictive processing in music by examining event-related potentials (ERPs) elicited by the violation of musical expectations. While several studies have reported that the predictability of stimuli can modulate the amplitude of ERPs, it is unclear how specific the representation of the expected note is. The present study addressed this issue by recording the omitted stimulus potentials (OSPs) to avoid contamination of bottom-up sensory processing with top-down predictive processing. Decoding of the omitted content was attempted using a support vector machine, which is a type of machine learning. ERP responses to the omission of four target notes (E, F, A, and C) at the same position in familiar and unfamiliar melodies were recorded from 25 participants. The results showed that the omission N1 were larger in the familiar melody condition than in the unfamiliar melody condition. The decoding accuracy of the four omitted notes was significantly higher in the familiar melody condition than in the unfamiliar melody condition. These results suggest that the OSPs contain discriminable predictive information, and the higher the predictability, the more the specific representation of the expected note is generated.
Cutaneous squamous cell carcinoma characterized by MALDI mass spectrometry imaging in combination with machine learning
Authors: Brorsen Lauritz F. , McKenzie James S. , Tullin Mette F. , Bendtsen Katja M. S. , Pinto Fernanda E. , Jensen Henrik E. , Haedersdal Merete , Takats Zoltan , Janfelt Christian , Lerche Catharina M.
Published: May 2024

Cutaneous squamous cell carcinoma (SCC) is an increasingly prevalent global health concern. Current diagnostic and surgical methods are reliable, but they require considerable resources and do not provide metabolomic insight. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) enables detailed, spatially resolved metabolomic analysis of tissue samples. Integrated with machine learning, MALDI-MSI could yield detailed information pertaining to the metabolic alterations characteristic for SCC. These insights have the potential to enhance SCC diagnosis and therapy, improving patient outcomes while tackling the growing disease burden. This study employs MALDI-MSI data, labelled according to histology, to train a supervised machine learning model (logistic regression) for the recognition and delineation of SCC. The model, based on data acquired from discrete tumor sections (n = 25) from a mouse model of SCC, achieved a predictive accuracy of 92.3% during cross-validation on the labelled data. A pathologist unacquainted with the dataset and tasked with evaluating the predictive power of the model in the unlabelled regions, agreed with the model prediction for over 99% of the tissue areas. These findings highlight the potential value of integrating MALDI-MSI with machine learning to characterize and delineate SCC, suggesting a promising direction for the advancement of mass spectrometry techniques in the clinical diagnosis of SCC and related keratinocyte carcinomas.
Predicting severe intraventricular hemorrhage or early death using machine learning algorithms in VLBWI of the Korean Neonatal Network Database
Authors: Kim Hyun Ho , Kim Jin Kyu , Park Seo Young
Published: May 2024

Severe intraventricular hemorrhage (IVH) in premature infants can lead to serious neurological complications. This retrospective cohort study used the Korean Neonatal Network (KNN) dataset to develop prediction models for severe IVH or early death in very-low-birth-weight infants (VLBWIs) using machine-learning algorithms. The study included VLBWIs registered in the KNN database. The outcome was the diagnosis of IVH Grades 3–4 or death within one week of birth. Predictors were categorized into three groups based on their observed stage during the perinatal period. The dataset was divided into derivation and validation sets at an 8:2 ratio. Models were built using Logistic Regression with Ridge Regulation (LR), Random Forest, and eXtreme Gradient Boosting (XGB). Stage 1 models, based on predictors observed before birth, exhibited similar performance. Stage 2 models, based on predictors observed up to one hour after birth, showed improved performance in all models compared to Stage 1 models. Stage 3 models, based on predictors observed up to one week after birth, showed the best performance, particularly in the XGB model. Its integration into treatment and management protocols can potentially reduce the incidence of permanent brain injury caused by IVH during the early stages of birth.
Microplastics and non-natural cellulosic particles in Spanish bottled drinking water
Authors: Gálvez-Blanca Virginia , Edo Carlos , González-Pleiter Miguel , Fernández-Piñas Francisca , Leganés Francisco , Rosal Roberto
Published: May 2024

This investigation explored the presence of microplastics (MPs) and artificial cellulosic particles (ACPs) in commercial water marketed in single use 1.5 L poly(ethylene terephthalate) bottles. In this work we determined a mass concentration of 1.61 (1.10–2.88) µg/L and 1.04 (0.43–1.82) µg/L for MPs and ACPs respectively in five top-selling brands from the Spanish bottled water market. Most MPs consisted of white and transparent polyester and polyethylene particles, while most ACPs were cellulosic fibers likely originating from textiles. The median size of MPs and ACPs was 93 µm (interquartile range 76–130 µm) and 77 µm (interquartile range 60–96 µm), respectively. Particle mass size distributions were fitted to a logistic function, enabling comparisons with other studies. The estimated daily intake of MPs due to the consumption of bottled water falls within the 4–18 ng kg−1 day−1 range, meaning that exposure to plastics through bottled water probably represents a negligible risk to human health. However, it's worth noting that the concentration of plastic found was much higher than that recorded for tap water, which supports the argument in favour of municipal drinking water.
Quantifying disparities in intimate partner violence: a machine learning method to correct for underreporting
Authors: Shanmugam Divya , Hou Kaihua , Pierson Emma
Published: May 2024

The first step towards reducing the pervasive disparities in women’s health is to quantify them. Accurate estimates of the relative prevalence across groups—capturing, for example, that a condition affects Black women more frequently than white women—facilitate effective and equitable health policy that prioritizes groups who are disproportionately affected by a condition. However, it is difficult to estimate relative prevalence when a health condition is underreported, as many women’s health conditions are. In this work, we present , a method for accurately estimating the relative prevalence of underreported health conditions which builds upon the literature in positive unlabeled learning. We show that under a commonly made assumption—that the probability of having a health condition given a set of symptoms remains constant across groups—we can recover the relative prevalence, even without restrictive assumptions commonly made in positive unlabeled learning and even if it is impossible to recover the absolute prevalence. We conduct experiments on synthetic and real health data which demonstrate ’s ability to recover the relative prevalence more accurately than do previous methods. We then use to quantify the relative prevalence of intimate partner violence (IPV) in two large emergency department datasets. We find higher prevalences of IPV among patients who are on Medicaid, not legally married, and non-white, and among patients who live in lower-income zip codes or in metropolitan counties. We show that correcting for underreporting is important to accurately quantify these disparities and that failing to do so yields less plausible estimates. Our method is broadly applicable to underreported conditions in women’s health, as well as to gender biases beyond healthcare.
An immunohistochemical atlas of necroptotic pathway expression
Authors: Chiou Shene , Al-Ani Aysha H , Pan Yi , Patel Komal M , Kong Isabella Y , Whitehead Lachlan W , Light Amanda , Young Samuel N , Barrios Marilou , Sargeant Callum , Rajasekhar Pradeep , Zhu Leah , Hempel Anne , Lin Ann , Rickard James A , Hall Cathrine , Gangatirkar Pradnya , Yip Raymond KH , Cawthorne Wayne , Jacobsen Annette V , Horne Christopher R , Martin Katherine R , Ioannidis Lisa J , Hansen Diana S , Day Jessica , Wicks Ian P , Law Charity , Ritchie Matthew E , Bowden Rory , Hildebrand Joanne M , O’Reilly Lorraine A , Silke John , Giulino-Roth Lisa , Tsui Ellen , Rogers Kelly L , Hawkins Edwin D , Christensen Britt , Murphy James M , Samson André L
Published: May 2024

Necroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell death in vivo has proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis regulators – Caspase-8, RIPK1, RIPK3 and MLKL – in formalin-fixed mouse and human tissues. We observed surprising heterogeneity in protein expression within tissues, whereby short-lived immune barrier cells were replete with necroptotic effectors, whereas long-lived cells lacked RIPK3 or MLKL expression. Local changes in the expression of necroptotic effectors occurred in response to insults such as inflammation, dysbiosis or immune challenge, consistent with necroptosis being dysregulated in disease contexts. These methods will facilitate the precise localisation and evaluation of necroptotic signaling in vivo.
A broadly applicable protein-polymer adjuvant system for antiviral vaccines
Authors: Wang Caiqian , Geng Yuanyuan , Wang Haoran , Ren Zeheng , Hou Qingxiu , Fang An , Wu Qiong , Wu Liqin , Shi Xiujuan , Zhou Ming , Fu Zhen F , Lovell Jonathan F , Jin Honglin , Zhao Ling
Published: May 2024

Although protein subunit vaccines generally have acceptable safety profiles with precise antigenic content, limited immunogenicity can lead to unsatisfactory humoral and cellular immunity and the need for vaccine adjuvants and delivery system. Herein, we assess a vaccine adjuvant system comprising Quillaja Saponaria-21(QS-21) and cobalt porphyrin polymeric micelles that enabling the display of His-tagged antigen on its surface. The nanoscale micelles promote antigen uptake and dendritic cell activation to induce robust cytotoxic T lymphocyte response and germinal center formation. Using the recombinant protein antigens from influenza A and rabies virus, the micelle adjuvant system elicited robust antiviral responses and protected mice from lethal challenge. In addition, this system could be combined with other antigens to induce high titers of neutralizing antibodies in models of three highly pathogenic viral pathogens: Ebola virus, Marburg virus, and Nipah virus. Collectively, our results demonstrate this polymeric micelle adjuvant system can be used as a potent nanoplatform for developing antiviral vaccine countermeasures that promote humoral and cellular immunity.
Synthesis of highly luminescent core–shell nanoprobes in a single pot for ofloxacin detection in blood serum and water
Authors: Kadian Pallavi , Singh Astha , Kumar Manish , Kumari Kanchan , Sharma Deepika , Randhawa Jaspreet Kaur
Published: May 2024

Carbon-doped silica shell-encapsulated iron oxide nanoparticles (CSIONPs) can detect ofloxacin (OLF) in tap water or blood serum. Upon excitation at 350 nm, CSIONPs exhibit optical properties leading to emission at 432 nm which is quenched upon the introduction of OLF.
External electric field driven electronic structures: tunable nonlinear optical properties of helical bilayer nanographenes
Authors: Gan Ping-yao , Huang Xiao , Gao Feng-wei , Xu Hong-liang
Published: May 2024

The external electric field regulates the charge transfer remarkably, which serves as an effective strategy for enhancing the NLO response of helical bilayer nanographenes.
CRISPR/Cas-based colorimetric biosensors: a promising tool for the diagnosis of bacterial foodborne pathogens in food products
Authors: Saleh Ebraheem Abdu Musad , Ali Eyhab , Muxamadovna Giyazova Malika , Kassem Asmaa F. , Kaur Irwanjot , Kumar Abhinav , Jabbar Hijran Sanaan , Alwaily Enas R. , Elawady Ahmed , Omran Alaa A.
Published: May 2024

Some physical phenomena and various chemical substances newly introduced in nanotechnology have allowed scientists to develop valuable devices in the field of food sciences.