bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Sr2+, BO33−, and SiO32− on differentiation of human dental pulp stem cells into odontoblast-like cells
Authors: Miyano Yuko , Mikami Masato , Katsuragi Hiroaki , Shinkai Koichi
Published: November 2022

Objectives: This study aimed to clarify the effects of strontium (Sr2⁺), borate (BO33−), and silicate (SiO32−) on cell proliferative capacity, the induction of differentiation into odontoblast-like cells (OLCs), and substrate formation of human dental pulp stem cells (hDPSCs).
Methods: Sr2+, BO33−, and SiO32− solutions were added to the hDPSC culture medium at three different concentrations, totaling nine experimental groups. The effects of these ions on hDPSC proliferation, calcification, and collagen formation after 14, 21, and 28 days of culture were evaluated using a cell proliferation assay, a quantitative alkaline phosphatase (ALP) activity assay, and Alizarin red S and Sirius red staining, respectively. Further, the effects of these ions on hDPSC differentiation into OLCs were assessed via real-time polymerase chain reaction and immunohistochemistry.
Results: Sr2+ and SiO32− increased the expression of odontoblast markers; i.e., nestin, DMP-1, dentin sialophospholipoprotein, and ALP genes, compared with the control group. BO33− increased the ALP gene expression and activity.
Significance: The results of this study suggested that Sr2+, BO33−, and SiO₃²− may induce hDPSC differentiation into OLCs.
Assay for interferon gamma release as a novel marker in pediatric patients with systemic lupus erythematosus
Authors: Zhang Song , Li Xue , Tian Yang , Chen Huishan , Liang Defeng , Chen Kaizhou , Cai Zhe , Zeng Huasong
Published: November 2022

Background: The interferon-gamma (IFN-γ) release assay (IGRA) is an important laboratory diagnosis for latent Mycobacterium tuberculosis (TB) infection. The TB-IGRA measures the release of IFN-γ from peripheral blood cells, who are exposed to TB antigen (Ag), mitogen (MT), or negative/nil control (NL) in vitro. While, an exceptional higher TB Ag-NL level will reflect an elevation of peripheral lymphocytes released IFN-γ in a same condition. Therefore, we found that the elevated levels of TB Ag-NL could become a new biomarker for the diagnosis and treatment of pediatric systemic lupus erythematosus (SLE) patients.
Methods: We have analyzed the clinical data of 776 children who are underwent TB-IGRA testing in the Department of Allergy and Rheumatology of Guangzhou Women and Children's Medical Center from 2018 to 2020. To investigate the association between TB Ag-NL and SLE, we have analyzed the clinical data of 47 SLE patients and TB Ag-NL testing results, and then evaluated the association between TB Ag-NL and SLE disease activity.
Results: The TB Ag-NL levels were significantly higher in patients with active SLE than those in inactive SLE (p=0.0002). The TB Ag-NL levels were positively correlated with the SLE disease activity index (SLEDAI) and laboratory diagnosis parameters. The mean value of TB Ag-NL in SLE patients (0.04191 ± 0.07955, IU/mL) were significantly higher than those in patients with juvenile dermatomyositis (JDM) (0.0158 ± 0.0337, IU/mL, p=0.036), juvenile idiopathic arthritis (JIA) (0.0162 ± 0.0388, IU/mL, p=0.001), and healthy controls (HC) (0.0001 ± 0.0027, IU/mL, p=0.0003). Therefore, the elevated TB Ag-NL levels could serve as a potential diagnostic biomarker of SLE, especially for the active SLE.
Conclusion: The detection of IFN-γ release levels by the TB-IGRA may be useful to assess SLE disease activity in pediatric patients with active SLE.
On the derivation of linear theories for gels
Authors: Barcellos Aline Rigueti , de Souza Angela Cristina Cardoso , Duda Fernando Pereira
Published: November 2022

In this paper we derive a coupled linear theory of elastic deformation and fluid permeation in gels. We start by introducing a nonlinear theory, which describe the state of a gel by its network deformation and interstitial fluid pressure, and then proceed with its linearization about an arbitrary and time-independent state. The linear theory so obtained accounts for the presence of residual stress and involves the tensors of elasticity and permeability which appear from the linearization of the responses for Piola stress and volumetric fluid flow. We present general expressions for these tensors in the case of isotropic gels and particularize the one for the elasticity tensor with respect to isotropic and transversely isotropic states. The linearized equations so obtained are applied to conduct a linear stability analysis of a gel layer attached to a rigid substrate to address the issue of swelling-induced wrinkling. The results are specialized for a gel whose elastic and mixing energies are described by the Gent and Flory–Huggins models.
Variation in dietary resilience among Amazonian populations living in a context of dramatic seasonal flooding
Authors: Tregidgo Daniel , Piperata Barbara , de Lima Jéssica Jaine Silva , Inglez Mariana , Valsecchi João
Published: November 2022

Every year, during one the planet’s greatest seasonal flooding events, Central Amazonian rivers inundate farmland and expand aquatic habitats, making fish harder to catch. Understudied is whether this hydrological regime impacts human dietary intakes/sufficiency, and, if so, whether it varies between communities in seasonally flooded (várzea) and non-flooded (terra firme) zones. To address this gap, we collected dietary data on 8,100 meals over the course of the hydrological cycle in six várzea and three terra firme communities, and calculated energy, carbohydrate, protein, and fat intake. Diets were dominated by fish (44%), the main source of protein and fat, and manioc flour (26%), the main source of energy and carbohydrate. Fish consumption fell as water levels rose, resulting in reduced energy and macronutrient intake in várzea communities. Terra firme communities showed greater seasonal resilience, maintaining protein and fat levels largely by increasing the consumption of wildmeat and fatty palm fruits, respectively.
Biodegradable starch sachets reinforced with montmorillonite for packing ZnO nanoparticles: solubility and Zn2+ ions release
Authors: Peres Laiza Gabriela Sanches , Malafatti João Otávio Donizette , Bernardi Bárbara , Mattoso Luiz Henrique Capparelli , Paris Elaine Cristina
Published: November 2022

Agriculture's importance in human lives and the economy has directed studies to improve crop production. An essential challenge for improving fertilizer efficacy is reducing losses due to leaching and increasing the nutrients supplies. In this context, biodegradable sachets stand out as internal packaging instead of direct insertion into the polymer matrix, facilitating the system processing and making it easier to adapt the soil's nutritional quantity. Thus, the present work aimed to increase the zinc oxide (ZnO) solubility by obtaining nanoparticles using top-down and bottom-up approaches and packaging them in montmorillonite (MMT) reinforced starch sachets. The different diameters and forms of the ZnO nanoparticles were evaluated to understand the solubility dependence on these parameters. In this way, the top-down process was performed for the attritor milling method allowing the nanoparticles with about 71 nm average diameter and greater homogeneity than the commercial one (approximately 174 nm). The milled ZnO nanoparticles presented better solubility than those synthesized bottom-up processes and the commercial ones, reaching a 90 to 100% solubility plateau in 48 h. Concerning starch sachets, the 1% MMT (w w− 1) insertion in the polymeric matrix promoted increased water vapor barrier and mechanical properties, improving the tensile strength. In the solubility test for nanoparticulate ZnO packed in sachets, similar behaviors to free ZnO were observed due to the high affinity of the starch matrix with water. Therefore, starch sachet systems with improved properties from the MMT reinforcement insertion showed as an alternative source of Zn2+ ions to minimize losses during application.
Photothermal seleno-polysaccharide polymer nanocomposites mediating mitochondria function to anti-tumor therapy
Authors: Yang Xiaotong , MA DANDAN , Sheng Enze , Tang Yingying , Tang Yuyin , Hu Linyu , Li Su , Zhu Dong
Published: November 2022

Mediating mitochondria function is essentially challenging but actually significance for biomedicine. However, remote non-invasive regulation of mitochondria function in a living system has been seldom demonstrated and further developed for potential therapy. In here, we prepared a seleno-polysaccharide polymer nanocomposite owning photothermal activity to increase anti-tumor therapy capacity. Such a nanocomposite comprises two key constitutions: near infrared polymer amphiphile and seleno-polysaccharide, acting as photothermal nanotransducer and temperature-sensitive reactive oxygen species (ROS) accelerator, respectively. Under near infrared irradiation (NIR), the productivity of intracellular reactive oxygen species (ROS) can be increased by 1.5-fold to efficiently mitochondrial dysfunction, leading to endogenous mitochondrial apoptosis. This design thus enables provide a promising approach to remotely mediate mitochondria-mediated apoptosis for anti-tumor therapy.
The numerical model of the Internal friction angle and Saturation Degree affect the vertical displacement of the ground
Authors: Doan Thy Truc
Published: November 2022

The numerical model of the different vertical displacement and the internal friction angle variations were determined carefully by the Viet Nam Standard. The difference between experimental values and simulation of the PLAXIS 3D software, was shown remarkably as the maximum value was shown in 0.01975 m (z = -36.9m) depth with ϕ0 = 290); whereas the minimum value in 0.002844 m (z = 0m) depth with ϕ0 = 20. The mean value at the center of the Clay layer (from 0.0m to 27.0m) depth was obtained at 0.0577m whereas compared with 0.0156 m at the Sand layer (from 27.0 to 39.6m) depths.
Moreover, water content (W%) and porosity (P%) were calculated particularly at the maximum value of 94.35% (borehole “HK 2”) with 4.8m depth, whereas the minimum value is 18.22% at 39.3m depth (borehole “HK3”); which compared with porosity (P%) of 71.9% (borehole “HK2”) at 4.8m depth, and 40.4% (borehole “HK3”) at 4.8m depth.
On the other hand, Saturation results presented a relatively low mean value of 88.09% and 86.79% at the center of the ground (from 4.3m to 15.3m). The increase of 23% and 75.42% (from 18.3m to 39.3m) as compared with the research of Zapatata, 1999.
The Rectum, Anal Sphincter and Puborectalis Muscle Show Different Contraction Wave Forms During Deferment of Simulated Feces
Authors: Sun Daming , Lo Kar Man , Chen Ssu-Chi , Leung Wing Wa , Wong Cherry , Mak Tony , Ng Simon , Futaba Kaori , Gregersen Hans
Published: November 2022

Introduction.
The contractile interaction of rectum, puborectalis muscle and anal sphincter must be studied to understand evacuation and consequences of deferred defecation.
Methods.
Six subjects were recruited to study contractile waveforms during deferred defecation with Fecobionics. The subjects filled out symptom questionnaires and had the balloon expulsion test and anorectal manometry done for reference. The Fecobionics bag was filled to urge-to-defecate volume in rectum. The subjects were asked to defer defecation for 4hrs. Pressures and bend angle variations were analyzed with Fast Fourier Transform (FFT). Two subjects were considered either constipated or hypersensitive.
Results.
The remaining four normal subjects were 23.8 ± 1.9yrs. Low frequency signals (< 0.06Hz) were found for pressures and bend angle. The waves were largely uncoordinated between recordings, except for rear and bag pressures. The frequency domain analysis showed waves with amplitude around 0.02-0.04Hz. Differences were found between the pressures and the bend angle both for peak 1 (ANOVA F = 9.403, p < 0.001) and peak 2 amplitudes (F = 5.48, p < 0.005). Post hoc tests showed that the front pressure amplitude was bigger than the others (rear and bend, p < 0.05; bag, p < 0.005) for peak 1, and bigger than bag pressure (p < 0.005) and bend angle (p < 0.05) for peak 2. The constipated subject had lower front pressure amplitudes compared to normal subjects. The other parameters had increased amplitudes. The hypersensitive subject also differed from normal subjects.
Conclusions.
The rectum, anal sphincter and puborectalis muscle showed different contraction waves during deferred defecation. The data call for larger studies to better understand the implications on anorectal disorders.
Real Time Efficient Block Level Dual Mode Data Deduplication Towards Mitigating Side Channel Attack in Cloud Storage
Authors: K Jayashree , K.E Narayana
Published: November 2022

The task of data deduplication and privacy preservation in cloud storage is well studied. There exist numerous approaches to enforce such deduplication and user privacy in cloud storage. However, the methods suffer to achieve expected performance in various metrics. To handle this issue, an efficient Real Time Block Level Dual Mode Data Deduplication (RBDD) scheme is presented in this article. The approach not just concentrates on data deduplication but also focuses on restricting the malicious adversaries at the block level. The method first enforces access restriction in block level based on the key metrics as well as access metrics measured in terms of Access Behavioral Measure (ABM). Also, the method maintains Page Matrix to store the schema of different pages which has information about the blocks, keys and encryption schemes. The dual mode approach encrypts the data encrypted by the user by its own system key which has been dynamically updated and modified at different sessions to handle different threats. The deduplication is performed by verifying the data in block level by computing Block Level Relational Measure (BLRM). Overall, the method improves the performance of data deduplication, public auditing and privacy preservation. Also, the method improves the performance in access restriction towards QOS development.
Improvement the Expression and Purification of Loa22: A Lipoprotein with OmpA domain from Pathogenic Leptospira Serovars
Authors: Gharakhani Mehdi , Ghasemi Mohammad Faezi , Khaki Pejvak , Esmaelizad Majid , Tebianian Majid
Published: November 2022

Background
Outer membrane proteins (OMPs) are the main surface antigens of the pathogenic Leptospires. One of the highly conserved outer membrane proteins expressed only by pathogenic Leptospires is Loa22. This study aims is to obtain the optimum conditions for high expression and purification of Loa22 recombinant (rLoa22) protein.
Methods
Based on evidence of phylogenetic studies, complete coding sequence of loa22 gene was optimized based on codon usage chart and sub-cloned into a pET32a (+) expression vector. BL21 (pLysS) was used as expression host for transformation. The recombinant clones were selected on ampicillin plates and subjected to PCR by using pET T7 primers and expression conditions optimized then by adjusting parameters such as culture media, induction time, temperature, and IPTG concentration.
Results
SDS-PAGE Analysis showed that the production of rLoa22 protein was at the highest level when post induction incubation, IPTG concentration, and duration of induction were 37ºC, 0.1M and 5h in 2xTY medium respectively. Due to the soluble nature of the protein, the purification of the rLoa22 protein under native conditions using Ni-NTA pull-down was optimum in one hour binding process at 37°C, five times washing process and elution buffer with a pH 7.4 and a 0.3 M imidazole concentration.
Conclusion
Based on the results of this study, optimizing the expression and purification process for over production of rLoa22 protein resulted in the large quantity of pure recombinant antigen that forms the basis for future investigation on the design of rapid diagnostic tests and more effective subunit vaccine candidates for leptospirosis.
Minimizing invasiveness and simplifying the surgical procedure for upper and middle early gastric cancer with near-infrared light and organ traction
Authors: Nagano Shinnosuke , Ushimaru Yuki , Kawabata Ryohei , Nishikawa Kazuhiro
Published: November 2022

Background
Surgeons are often faced with optimal resection extent and reconstructive method problems in laparoscopic gastrectomy for gastric cancer in the upper and middle body of the stomach. Indocyanine green (ICG) marking and Billroth I (B-I) reconstruction was used to solve these problems with the organ retraction technique.
Case Presentation
A 51-year-old man with upper gastrointestinal endoscopy revealed a 0-Ⅱc lesion in the posterior wall of the upper and middle gastric body 4 cm from the esophagogastric junction. CT1bN0M0 (cStage IA) was the preoperative diagnosis. Laparoscopic distal gastrectomy (LDG) and D1 + lymphadenectomy was decided to be performed considering postoperative gastric function preservation. The ICG fluorescence method was used to determine the accurate tumor location since the determination was expected to be difficult to the extent of optimal resection with intraoperative findings. By mobilizing and rotating the stomach, the tumor in the posterior wall was fixed in the lesser curvature, and as large a residual stomach as possible was secured in gastrectomy. Finally, delta anastomosis was performed after increasing gastric and duodenal mobility sufficiently. Operation time was 234 min and intraoperative blood loss was 5 ml. The patient was discharged 10 days postoperatively without complications.
Conclusion
The indication for LDG and B-I reconstruction can be expanded to cases where laparoscopic total gastrectomy or LDG and Roux-en-Y reconstruction has been selected for early-stage gastric cancer in the upper gastric body by combining preoperative ICG markings and gastric rotation method dissection.
Brain injury after cranial radiotherapy combined with immunotherapy for brain metastases in lung cancer: a retrospective study
Authors: Li Jiatong , Li Wanhu , Xu Shuhui , Li Yuying , Lu Shuangqing , Chen Feihu , Yu Jinming , Zhu Hui
Published: November 2022

Background: For patients with brain metastasis (BM) from lung cancer, whether cranial radiotherapy (CRT) combined with immune checkpoint inhibitors (ICIs) will increase the risk of radiation-induced brain injury (RBI) remains inconclusive. This retrospective study was performed with the aim of analyzing the incidence of RBI of CRT combined with ICIs and revealing the risk factors forRBI.
Methods: We retrospectively reviewed the medical records of patients with BM from lung cancer who underwent CRT between January 2019 and December 2020 at Shandong Cancer Hospital and Institute. According to whether systemic treatment was used within 6 months before and after CRT, all patients were divided into theCRT+ICIs group and the CRT+non-ICIs group respectively. The diagnosis of brain radiation-induced necrosis (RN) and white matter lesions (WML) was evaluated by brain enhanced MRI. The Fazekas scale and CTCAE v5.0 were used to grade the injury. The risk factors for RBI were identified using univariate and multivariate analyses.
Results: Overall, 210 BM patients undergoing CRT were included in our study. Within 6 months before and after CRT, 56 patients received ICIs, and 154 patients received other systemic therapeutic drugs, including tyrosine kinase inhibitors (TKIs) and chemotherapy. Seventeen (8.1%) patients developed RN, and 142 (67.6%) patients developed WML. The incidence of RN in theCRT+ICIs group vs. the CRT+non-ICIs group was 14.3% vs. 5.8% (p=0.090). However, if ICIs were used within three months of CRT, the incidence of RN in the CRT+ICIs group was significantly higher than that in the CRT+non-ICIs group (18.5% vs. 5.4%, p=0.045).
Multivariate analysis revealed that the maximum diameter of BM > 3.3 cm (p = 0.023) and the total cumulative radiation dose of metastatic lesions > 75.7 Gy (p = 0.018) were risk factors for RN. Additionally, re-radiotherapy was also a trend factor in the development of RN (OR 3.40; 95% CI 0.99-11.67, p=0.051).
Conclusions: ICIs could increase the risk of RN, especially when used within three months of CRT. The total cumulative radiation dose of metastatic lesions is closely related to the development of RN, and re-radiotherapy is also a trend factor in the development of RN.
Multimodal Imaging of Presumed Dusn Associated with Strongyloides Stercoralis Infection
Authors: Urbano Adela , Giralt Lena , Artaraz Joseba , Fonollosa Alex
Published: November 2022

PURPOSE
To report multimodal imaging findings in a case of presumed Diffuse Unilateral Subacute Neuroretinitis (DUSN) associated with Strongyloides stercoralis infection.
METHODS
Retrospective case report.
RESULTS
A thirty-eight-year-old woman presented with visual loss of one week’s duration in her left eye. She had a medical history of chronic dermatitis and epigastralgia. Fundoscopic examination revealed diffuse subretinal inflammatory lesions. Optical coherence tomography showed a disruption of the ellipsoid layer and focal thickenings of the retinal pigment epithelium in the macular region, as well as cells in the vitreous cavity. Fundus autofluorescence showed granular hyperautofluorescence in the posterior pole. Fluorescein angiography revealed leakage of the optic disk and pinpoint disperse hyperfluorescent lesions. Serology tests detected Strongyloides stercoralis antibodies and blood count showed eosinophilia. Treatment with Ivermectine resolved both the ophthalmologic abnormalities and the eosinophilia.
CONCLUSION
DUSN may be associated with Strongyloides stercoralis infection. Inflammatory lesions, if treated early, may resolve without sequelae.
Use of hydrogeochemistry and environmental isotopes for evaluation of groundwater in Qinlian coal base of China
Authors: Zhao Chongqin , Li Xiangquan , Wang Zhenxing , Hou Xinwei , Ma Jianfei
Published: November 2022

The Jiangcang Basin is an important mining area of the former Qilian Mountain large coal base in Qinghai Province. In this study, hydrogeochemical specifications and multiple isotopic tracers are used to propose the main controlling factors of groundwater evolution and groundwater circulation mechanism in the Jiangcang Basin. According to different ion ratios to reveal the main hydrogeochemical processes of groundwater in the study area. The δ18O and the δD isotopes distribution show that suprapermafrost water is recharged by modern meteoric water, while infrapermafrost water is recharged by modern meteoric at the time of nuclear explosion, and the groundwater is closely hydraulically connected to the surface water with weak evaporation overall. Based on the δ34S isotopic characteristics, it is concluded that the high δ34S value of deep groundwater is due to SO4 reduction, and SO42−-rich snow recharge with lixiviated sulfate minerals are the main controlling factor for the high SO42− concentration in groundwater. In addition, using the 14C and the 3H isotopes to date groundwater, we find that the formation age of suprapermafrost water is between 7 and 21 years, that of infrapermafrost water is more than 46 years, that of hot springs is between 8305 and 39600 years, while the formation age of infrapermafrost deep water in the tectonic talik is between 2537 and 6889 years, which has faster updating capacity than thermal springs. According to the multivariate water conversion relationships, it reveals that the river receives more groundwater recharge, suprapermafrost water is recharged by the proportion of meteoric water, which is closely related to the mountainous area at the edge of the basin, while infrapermafrost water is mainly recharged by the shallow groundwater. Through detailed hydrogeological investigation and hydrogeochemical isotope characterization, the groundwater circulation model of the Jiangcang Basin is constructed. The research results are of great significance to the sustainable utilization of groundwater and ecological environmental protection in the coal base of the Tibetan Plateau.
Superficial angiomyxoma of the breast in a 25-year-old woman without carney’s complex: case report and literature review
Authors: Meng Tian , Yin Haoqiang , Chen Ling , Wu Jingjing , Cheng Yiqin , Wang Song , Zhao Qiufeng
Published: November 2022

Background:Superficial angiomyxoma (SAM) is a rare, benign tumor characterized by scattered stellate or spindle cells and thin-walled blood vessels embedded in abundant myxoid stroma. It was first reported as a cutaneous myxoma associated with Carney complex in 1985, and lesions are commonly located in the eyelids, external ear canal, and nipples and often appear in multiples. In cases with no evidence of Carney complex, the incidence of breast SAM is extremely rare.Case presentation:We reported a case of a 25-year-old Chinese woman presented with a left breast lump in the subareolar region for three years and gradually increased in size. No evidence of Carney complex was noted. Breast ultrasonography (US) and magnetic resonance imaging (MRI) were undertaken with US BI-RADS 4A and MR BI-RADS 4, respectively. The patient denied biopsy and underwent a lumpectomy and was diagnosed with SAM. There was no tumor recurrence after 18 months of follow-up.Conclusion:This case report suggests that the preoperative US and MRI characteristics of breast SAM are specific and in accordance well with the histological appearances. Recognition of these imaging features may be helpful for the diagnosis of SAM. And complete excision and long-time regular follow-up after surgery are recommended.
Composition of Pseudo-Differential Operators involving the Weinstein Transform
Authors: Upadhyay S. K. , Sartaj Mohd
Published: November 2022

Boundedness results of the composition of two pseudo-differential operators involving the Weinstein transformin Weinstein Sobolev space H0,1α are investigatedby utilizing the theory of Weinstein transform. Some special cases are discussed regarding the composition of two pseudo-differential operators and the estimation of the solution of the heat equation is done by taking the Weinstein transform theory.
MSC Classification: 46F12, 35S05, 47G30, 46E35, 44A35
Comparative study between the efficacy and safety of topical sildenafil citrate solution and topical minoxidil 5%in the treatment of female pattern hair loss .
Authors: Mostafa Heba Abdo , Mohammed Hassan Abou Khodair , Fouda Ibrahim
Published: November 2022

Background: Female pattern hair loss (FPHL) is a nonscarring progressive thinning of hair with gradual decrease in it's number , especially in the frontal, central, and parietal scalp. the loss of terminal hairs in affected areas is usually incomplete and the frontal hairline is often spared. It is caused by follicular miniaturization, a process that gradually reduces the proportion of terminal to vellus hair .
Aim of the work: to evaluate the efficacy and safety of topical sildenafil citrate 1 % solution in comparison with topical minoxidil 5% foam in the treatment of female pattern hair loss .
Patients and methods: This exploratory pilot study included 30 female patients suffering from female pattern hair loss. Included patients were divided into 2 groups ; group1: received 1% topical sildenafil citrate solution and group2: received 5% topical minoxidil foam. Assessment of treatment response was done using trichoscopy.
Results: In group 1(treated with topical sildenafil 1%):There was a statistically significant increase in the vellus hair count in the frontal region, vertex and temporal side after treatment as compared to before treatment. Also, there was statistically significant increase in the terminal hair count in the frontal region and vertex region after treatment as compared to before treatment. There was no statistically significant difference in the hair thickness in all the regions after treatment. , Group 2(treated with topical minoxidil 5% ) there was a statistically significant increase in the Terminal hair count and hair thickness in the frontal region, vertex and temporal side after treatment as compared to before treatment. Also, there was statistically significant decrease in the vellus hair count, but it was detected only in the frontal region.
Conclusion: topical sildinafil 1% treatment proves to be a good alternative in the treatment of FPHL, although topical minoxidil 5% is still the first choice in FPHL treatment.
Transcription analysis of the THBS2 gene through regulation by potential non-coding diagnostic biomarkers and oncogenes of gastric cancer in the ECM receptor interaction signaling pathway: integrated systems biology and experimental investigation
Authors: Barani Ali , Beikverdi Kamyar , Mashhadi Benyamin , Parsapour Naeimeh , Rezaei Mohammad , Azadeh Mansoureh
Published: November 2022

Background
Gastric cancer (GC) is the second most frequent cause of cancer-related death worldwide and the fourth most common malignancy. Despite significant improvements in patient survival over the past few decades, the prognosis for patients with GC remains dismal because of the high recurrence rate. In this comprehensive systems biology and experimental investigation, we aimed to find new novel diagnostic biomarkers of GC through a regulatory RNA interaction network.
Methods
Gene expression, co-expression, and survival analyses were performed using microarray and RNAseq datasets (analyzed by R Studio, GEPIA2, and ENCORI). RNA interaction analysis was performed using miRWalk and ENCORI online databases. Gene set enrichment analysis (GSEA) was performed to find related signaling pathways of up and down-regulated genes in the microarray dataset. Gene ontology and pathway enrichment analysis were performed by the enrichr database. Protein interaction analysis was performed by STRING online database. Validation of expression and co-expression analyses was performed using a qRT-PCR experiment.
Results
Based on bioinformatics analyses, THBS2 (FC: 7.14, FDR < 0.0001) has a significantly high expression in GC samples. lncRNAs BAIAP2-AS1, TSIX, and LINC01215 have RNA interaction with THBS2. BAIAP2-AS1 (FC: 1.44, FDR: 0.018), TSIX (FC: 1.34, FDR: 0.038), and LINC01215 (FC: 1.19, FDR: 0.046) have significant up-regulation in GC samples. THBS2 has a significant role in the regulation of the ECM-receptor signaling pathway. miR-4677-5p has a significant RNA interaction with THBS2. The expression level of THBS2, BAIAP2-AS1, TSIX, and LINC01215 has a non-significant negative correlation with the survival rate of GC patients (HR: 0.28, logrank p: 0.28). qRT-PCR experiment validates mentioned bioinformatics expression analyses. BAIAP2-AS1 (AUC: 0.7136, p-value: 0.0096), TSIX (AUC: 0.7456, p-value: 0.0029), and LINC01215 (AUC: 0.7872, p-value: 0.0005) could be acceptable diagnostic biomarkers of GC.
Conclusion
BAIAP2-AS1, lncRNA LINC01215, lncRNA TSIX and miR-4677-5p might modulate the ECM-receptor signaling pathway via regulation of THBS2 expression level, as the high-expressed non-coding RNAs in GC. Furthermore, mentioned lncRNAs could be considered potential diagnostic biomarkers of GC.
Entre la política y la economía: las políticas de industrias culturales en Santiago de Cali, Colombia
Authors: Garcés Angulo Hailyn Yuranny
Published: November 2022

Santiago de Cali es uno de los epicentros multiculturales de Colombia y Latinoamérica, una ciudad eje de las industrias culturales en el país que se sostiene, en parte, debido a un grupo sistemático de incentivos y restricciones generados preponderantemente desde el Estado, un conjunto que se denomina en este trabajo como políticas culturales. El objetivo del artículo es identificar los lineamientos conceptuales de las políticas culturales en Cali, a partir de un ejercicio de revisión documental de la literatura sobre este tema, con el fin de entender las tendencias que guían su diseño, ejecución y evaluación, pues estas producen impactos socioeconómicos profundos en el complejo ecosistema cultural. Como se observa, el resultado principal que arroja esta hermenéutica documental es la predominancia de ideas provenientes de la economía de la cultura, las cuales están inclinando las políticas culturales hacia un enfoque económico utilitarista de corte extractivo, una tendencia que puede ocasionar impactos profundos en las condiciones socioeconómicas del sistema cultural caleño.
Effectiveness of the Family Education Program on Protection from Neglect and Abuse Offered to Families with Children with Disabilities
Authors: YILDIRIM Sevgi , ATBAŞI Zehra
Published: November 2022

Introduction: This study was conducted to test the effectiveness of a family education program on the prevention of neglect and abuse, which is offered to families with children with disabilities, on the knowledge levels of families about neglect and abuse. In the study, a family education booklet prepared by the researchers regarding the prevention of neglect and abuse in the training process was used. The content of the family education booklet was organized into presentations and were presented in five sessions.Method: This research was carried out using a mixed methods approach in which both quantitative and qualitative findings were used. In the study, a pretest-post-test experimental design with experimental and control groups was used to collect the quantitative data. The same tests were applied to the experimental group three weeks after the training, and monitoring data were obtained. In addition, an interview form consisting of four questions prepared by the researcher was applied to the experimental group immediately after the training, and the opinions of the families about the education were captured. The quantitative data of the research were analyzed using a statistical software program. The research was conducted with a total of 40 parents with children with disabilities, with 20 participants in the experimental group and 20 participants in the control group.Results: It was observed that the family education program on protection from neglect and abuse offered to families with children with disabilities was effective in increasing the level of knowledge of families on neglect and abuse. From the findings of this study, it was concluded that the effectiveness of the family education program, which was also presented in the follow-up tests applied to the families three weeks later, was preserved.Discussion: While the families had a low level of knowledge about neglect and abuse before the training, there was an increase in their knowledge level after the training. It is thought that if families are informed about this issue, they will be wiser about their children’s protection from neglect and abuse. Therefore, this study will serve the purpose of assisting to prevent neglect and abuse.
Socio-religious Practices of Kalang Shaman: Symbol of Minority People's Resistance in Indonesia
Authors: Kholiq Abdul , Ismail Ahmad , Permata Kartika Indah , Ali Md Sikandar
Published: November 2022

Three possibilities can occur when religion enters a society already having its own culture and traditions: acceptance, rejection, and adjustment. This study analyzed the socio-religiosity of the Kalang people, a minority ethnic group in Kendal, following the arrival of Islam into their lives. Highlighting how they practiced their worship and presented their identity in the majority of Muslim society, the research was focused on the socio-religious practices of the Dukun Kalang as a representation of Kalang people and unveiled her critical roles in the Kalang socio-cultural system, such as leading every ritual activity, and being a source of knowledge about Kalang teachings and other mystical information about ancestral spirits. Using the qualitative method, it was found that the Kalang shaman's religiosity model symbolized the way of religion without losing faith in indigenous culture and traditions. This spiritual adaptation model, in the perspective of symbolic interaction, can be interpreted as a form of resistance and as a strategy to maintain the cultural identity of the minority amid the threat of extinction due to the penetration of major ideologies, including mainstream religions.
On the issue of the motion of balls in a double pendulum
Authors: Strautmanis Guntis , Schukin Igors , Filimonikhin Gennadiy , Mezitis Mareks , Carjova Kristine
Published: November 2022

The paper considers a model of a vertical double pendulum with one suspension centre moving in a vertical plane. For the proposed system of pendulums, differential equations of motion and conditions for the collision of balls are obtained. When modelling the movement of pendulums, the central impact of the balls was considered for various variants of the movement of the suspension point: the suspension point oscillates in the vertical direction; the suspension point makes rotational movements in the vertical plane. In this case, various conditions of the central impact between the balls were considered: absolutely inelastic impact; absolutely elastic impact; impact with the transformation of impact energy (elastic impact). Comparison of the results of the numerical simulation and the results of experiments with the Kapitza pendulum in published sources confirmed the possibility of modelling an elastic impact between balls in a double pendulum and between balls in an autobalancer with a horizontal axis of rotation of the rotor.
Damage localization in beams based on the analysis of modal parameters
Authors: Sha Ganggang , Cao Maosen , Xiao Wen , Radzieński Maciej , Zuo Hongfu
Published: November 2022

This paper presents a two-step method for damage localization in beams by combining natural frequencies and mode shapes. The general locations of the damage are first identified from an indicator developed using relative natural frequency change (RNFC) curves and the values of RNFCs. A curvature-mode-shape-based method is then utilized to determine the specific location of the damage in the second step. The proposed two-step method is verified by detecting damage in a simulated simply-supported beam. The identified damage location agrees well with the actual damage location. A strategy for fast and accurate damage localization based on general localization using natural frequencies and specific localization using mode shapes is the main novelty of the paper.
Oxygen Binding to α-Subunits in High-Salt Crystals of t-State Hemoglobin
Authors: Frontice Clara , Gilliland Gary L. , Vasquez Gregory B. , Ji Xinhua , Pechik Igor
Published: November 2022

The crystal structures of a partially-oxygenated, human, T-state hemoglobin variant, βC112G, and a deoxy structure of a related hemoglobin construct, β (C93A+C 112G), have been determined at 2.0 A and 1.8 A resolution, respectively. Oxygen molecules fully occupy the, α -heme and partially occupy the a.,-heme binding sites of the βC112G mutant hemoglobin. Comparisons of these structures with deoxy-, oxy-, and carboxyhemoglobin provide insight into hemoglobin's allosteric transition.
Improved Crystals of Bovine Neurophysin II
Complexed with Vasopressin
Authors: Hu Bing , Rose John P. , Newton M. Gary , Breslow Esther , Zheng Changsheng , Wang Bi-Cheng
Published: November 2022

A modified bovine neurophysin II in which the first six residues have been removed by digestion has been crystallized with the hormone vasopressin. The crystals belong to space group P6,22 (or P6322) with unit cell
constants of a = b = 50,9A, c = 105.8 A. Crystals of the vasopressin complex have one subunit of the complex
per asymmetric unit, which corresponds to a solvent content of approximately 40%. The crystals diffract to beyond 2.3A resolution, which is substantially better than the 4 A diffraction reported for the full-length neurophysin-vasopressin complex reported previously.
Development of Silver Nanoparticles Green-formulated by Matricaria Chamomilla as Novel Chemotherapeutic Nanoformulation for the Treatment of Oral Squamous Cell Carcinoma
Authors: Yang Rui , Wang Mingguo , Ma Xiaoxia , Gao Qing
Published: November 2022

Aim:

To develop modern chemotherapeutic nanoformulation from plant extract to treat oral squamous cell carcinoma.

Background:

The use of biodegradable polymers to deliver drugs via nanoparticles solves a number of issues. AgNPs nanoparticle composites could be a promising material with applications in biological and pharmaceutical sciences. The biomolecules in the extract give the AgNPs additional stability against oxidation and corrosion. As a result, we are interested in reporting the synthesis, characterization, and uses of unique AgNPs decorated with Matricaria chamomilla extract.

Objective:

We developed a natural chemotherapeutic nanoformulation containing M. chamomilla aqueous extract and silver nanoparticles (AgNPs) for treating oral squamous cell carcinoma.

Methods:

UV–Visible Spectroscopy (UV-Vis), Fourier Transformed Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), and Field Emission Scanning Electron Microscopy (FESEM) were used to characterize AgNPs. The antioxidant activities of AgNO3, M. chamomilla, and AgNPs were evaluated using the DPPH assay in the presence of Butylated Hydroxytoluene (BHT) as a positive control. The MTT assay was employed on the HSC-4, Ca9-22, and HSC-3 cell lines to assess the cytotoxicity and anti-oral squamous cell carcinoma effects.

Results:

Silver nanoparticles demonstrated reduced cell viability and anti-oral squamous cell carcinoma capabilities in HSC-4, Ca9-22, and HSC-3 cell lines in a dose-dependent manner, with minimal damage to the normal cell line. The HSC-3 cell line showed the strongest anti-oral squamous cell carcinoma characteristics of AgNPs when tested against the above cell lines.

Conclusion:

According to the findings, silver nanoparticles containing M. chamomilla aqueous extract may treat different forms of oral squamous cell carcinoma in people.
Computational Fluid Dynamics: Insights and Applications in the
Pharmaceutical Field
Authors: Singh Vanshita , Shah Kamal , Garg Akash , Dewangan Hitesh Kumar
Published: November 2022

Abstracts:

Computational fluid dynamics (CFD) is a feasible tool to examine and troubleshoot different
types of equipment utilized in the pharmaceutical industry or healthcare. As a large number of fluids are
processed by unit operations, even some increments in performance and efficiency may escalate profits
and reduce costs. CFD methods are primarily used in the automobile and aerospace industries, but in the
current era, this technology is extensively applied in the pharmaceutical and chemical industries and has
become an important tool for process design scaleup and optimization. CFD is a numerical approach utilizing
CFD software to solve equations numerically. This review focuses on the diverse utilization of
CFD in the pharmaceutical field and current applications in the COVID 19 pandemic, a recent health
crisis that is intimidating the world.
Cytotoxic and Apoptotic Impacts of Ceranib-2 on RAW 264.7 Macrophage Cells
Authors: Alanyalı filiz Susuz , Algı Osman
Published: November 2022

Background:

Many ceramidase inhibitors have been developed and identified as potential treatment agents
for various types of tumors in the last several decades. In recent years, their therapeutic potential against tumors has
gained great attention. Inhibition of ceramidase is r eportedly related to apoptosis and cytotoxicity in macrophages,
which are closely related to tumor development and progression. However, whether and how ceranib-2, a novel
ceramidase inhibitor, can exert its cytotoxic and apoptotic effects on RAW 264.7, a macrophage cell line established
from a tumor in a male mouse induced with the Abelson murine leukemia virus, remains unknown.

Objective:

In this study, we aimed to investigate whether and how ceranib-2 can exert cytotoxic, antiproliferative, and
apoptotic effects on the RAW264.7 macrophages.

Methods:

We performed the MTT assay, Annexin V staining assay, and confocal microscopy to detect the cytotoxicity,
apoptosis, and morphological changes, respectively, in the RAW264.7 cells.

Results:

The viability of RAW264.7 cells treated with ceranib-2 was decreased as the doses of ceranib-2 increased at
24 h and 48 h due to apoptosis resulting from ceranib-2-reduced integrity of the mitochondrial membrane. Moreover,
morphological changes were observed in these ceranib-2 exposed cells, further indicating the role of ceranib-2 in inducing
apoptosis in these cells.

Conclusion:

Ceranib-2 is cytotoxic to RAW 264.7 macrophages and can induce apoptosis in these cells.
Development and Evaluation of Luliconazole Nail Lacquer Containing
Potential Permeation Enhancers for an Enhanced Transungual Drug
Delivery
Authors: Chauhan Preeti , Pahwa Rimpy , Kumari Pooja , Kumar Tarun , Ahuja Munish
Published: November 2022

Background:

Topical therapy for nail infection has a serious drawback of drug permea-tion via keratinized human nail plate. Onychomycosis, or tinea unguium, is a human nail fungal infection affecting nearly 19% of the world’s population.

Aim:

Purposely, we aimed to develop and evaluate nail lacquer formulations incorporated with lu-liconazole utilizing different permeation enhancers for targeted transungual drug delivery. Nail clippings were treated with luliconazole solution (5% w/v) with or without permeation enhancers and screened by determining the hydration enhancement factor and retention of the drug in the nail clippings. Different batches of nail lacquer formulations were prepared to employ Eudragit, poly-ethylene glycol 400, Tween 80, and permeation enhancer with the highest hydration enhancement factor value.

Methods:

Successively, the formulations were evaluated for studies like compatibility, application, gloss, blush, smoothness of flow, adhesion, non-volatile content, etc. Based on the results of physi-cal characterization and in vitro release study, formulations based on Eudragit RLPO and RSPO containing N-acetylcysteine and urea and the Eudragit RL100 containing urea as penetration en-hancer were found to be potentially useful. Furthermore, a comparative ex vivo evaluation of the formulations for permeation across the nail clippings revealed that the luliconazole-loaded Eudragit RLPO formulations containing N-acetylcysteine and urea provided the highest flux (5.12 μg/cm2/min) and shortest lag time (17.4 min).

Results:

Morphological analysis showed an increase in the number of pores on the nail surface, leading to the enhancement of drug diffusion across the nail matrix and nail bed.

Conclusion:

Furthermore, the luliconazole nail lacquer formulation exhibited higher antifungal ac-tivity, viscosity, and stability properties. Hence, the results suggest that the developed luliconazole nail lacquer formulation is an efficient topical transungual drug delivery system.
Recent Progress on the Application of the Polyaniline-Pd Catalysts for C-C
Cross-coupling Bond Forming Reactions: Trend and Future Analysis
Authors: Roy Moumita
Published: November 2022

In this review recent progress on the application of the polyaniline supported palladium catalysts in different organic transformations focusing on different C-C bond forming reactions such as Suzuki coupling, Heck reactions, oxidative Heck coupling, Ullmann coupling, Sonogashira and related chemistry. Effect of catalyst preparation, characteristic of the support and supported palladium species on the outcome of the catalyst efficiency are also highlighted. Finally, the emerging trend is summarized for the future of this unique modular catalytic system.
Review on the Importance of Chitosan in Different Pharmaceutical
Applications
Authors: Alkadi Hourieh , Allaf Abdul Wahab
Published: November 2022

Chitosan is an amino-polysaccharide polymer that has a unique structure, multi properties,
highly sophisticated functionality and a wide range of applications in biomedical and pharmaceutical
research as well as other industrial applications in connection with both pharmaceutical and
medical fields. Additionally, it appears that this unique material can be emphasized as a good candidate
for drugs variety carrier, drug release applications, and ocular and antimicrobial applications
including treatment of diabetes. This review highlights the importance and pharmaceutical applications
of chitosan in different fields of research and applications.