bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Fluid Flow Shear Stress on Osteoblast Differentiation and Cross-Talk with Articular Chondrocytes
Authors: Hinton Paige V. , Genoud Katelyn J. , Early James O. , O’Brien Fergal J. , Kennedy Oran D.
Published: August 2022

Bone cells, in particular osteoblasts, are capable of communication with each other during bone growth and homeostasis. More recently it has become clear that they also communicate with other cell-types; including chondrocytes in articular cartilage. One way that this process is facilitated is by interstitial fluid movement within the pericellular and extracellular matrices. This stimulus is also an important mechanical signal in skeletal tissues, and is known to generate shear stresses at the micron-scale (known as fluid flow shear stresses (FFSS)). The primary aim of this study was to develop and characterize an in vitro bone–cartilage crosstalk system, to examine the effect of FFSS on these cell types. Specifically, we evaluated the response of osteoblasts and chondrocytes to FFSS and the effect of FFSS-induced soluble factors from the former, on the latter. This system will ultimately be used to help us understand the role of subchondral bone damage in articular cartilage degeneration. We also carried out a comparison of responses between cell lines and primary murine cells in this work. Our findings demonstrate that primary cells produce a more reliable and reproducible response to FFSS. Furthermore we found that at lower magnitudes , direct FFSS produces anabolic responses in both chondrocytes and osteoblasts, whereas higher levels produce more catabolic responses. Finally we show that exposure to osteoblast-derived factors in conditioned media experiments produced similarly catabolic changes in primary chondrocytes.
Decoding Functional High-Density Lipoprotein Particle Surfaceome Interactions
Authors: Frey Kathrin , Goetze Sandra , Rohrer Lucia , von Eckardstein Arnold , Wollscheid Bernd
Published: August 2022

High-density lipoprotein (HDL) is a mixture of complex particles mediating reverse cholesterol transport (RCT) and several cytoprotective activities. Despite its relevance for human health, many aspects of HDL-mediated lipid trafficking and cellular signaling remain elusive at the molecular level. During HDL’s journey throughout the body, its functions are mediated through interactions with cell surface receptors on different cell types. To characterize and better understand the functional interplay between HDL particles and tissue, we analyzed the surfaceome-residing receptor neighborhoods with which HDL potentially interacts. We applied a combination of chemoproteomic technologies including automated cell surface capturing (auto-CSC) and HATRIC-based ligand–receptor capturing (HATRIC-LRC) on four different cellular model systems mimicking tissues relevant for RCT. The surfaceome analysis of EA.hy926, HEPG2, foam cells, and human aortic endothelial cells (HAECs) revealed the main currently known HDL receptor scavenger receptor B1 (SCRB1), as well as 155 shared cell surface receptors representing potential HDL interaction candidates. Since vascular endothelial growth factor A (VEGF-A) was recently found as a regulatory factor of transendothelial transport of HDL, we next analyzed the VEGF-modulated surfaceome of HAEC using the auto-CSC technology. VEGF-A treatment led to the remodeling of the surfaceome of HAEC cells, including the previously reported higher surfaceome abundance of SCRB1. In total, 165 additional receptors were found on HAEC upon VEGF-A treatment representing SCRB1 co-regulated receptors potentially involved in HDL function. Using the HATRIC-LRC technology on human endothelial cells, we specifically aimed for the identification of other bona fide (co-)receptors of HDL beyond SCRB1. HATRIC-LRC enabled, next to SCRB1, the identification of the receptor tyrosine-protein kinase Mer (MERTK). Through RNA interference, we revealed its contribution to endothelial HDL binding and uptake. Furthermore, subsequent proximity ligation assays (PLAs) demonstrated the spatial vicinity of MERTK and SCRB1 on the endothelial cell surface. The data shown provide direct evidence for a complex and dynamic HDL receptome and that receptor nanoscale organization may influence binding and uptake of HDL.
Transcriptomics Integrated with Metabolomics: Assessing the Central Metabolism of Different Cells after Cell Differentiation in Aureobasidium pullulans NG
Authors: Zeng Nan , Zhang Ning , Ma Xin , Wang Yunjiao , Zhang Yating , Wang Dandan , Pu Fangxiong , Li Bingxue
Published: August 2022

When organisms are stimulated by external stresses, oxidative stress is induced, resulting in the production of large amounts of reactive oxygen species (ROS) that inhibit cell growth and accelerate cellular aging until death. Understanding the molecular mechanisms of abiotic stress is important to enhance cellular resistance, and Aureobasidium pullulans, a highly resistant yeast-like fungus, can use cellular differentiation to resist environmental stress. Here, swollen cells (SCs) from two different differentiation periods in Aureobasidium pullulans NG showed significantly higher antioxidant capacity and stress defense capacity than yeast-like cells (YL). The transcriptome and the metabolome of both cells were analyzed, and the results showed that amino acid metabolism, carbohydrate metabolism, and lipid metabolism were significantly enriched in SCs. Glyoxylate metabolism was significantly upregulated in carbohydrate metabolism, replacing the metabolic hub of the citric acid (TCA) cycle, helping to coordinate multiple metabolic pathways and playing an important role in the resistance of Aureobasidium pullulans NG to environmental stress. Finally, we obtained 10 key genes and two key metabolites in SCs, which provide valuable clues for subsequent validation. In conclusion, these results provide valuable information for assessing central metabolism-mediating oxidative stress in Aureobasidium pullulans NG, and also provide new ideas for exploring the pathways of eukaryotic resistance to abiotic stress.
Mycoremediation of Flotation Tailings with Agaricus bisporus
Authors: Budzyńska Sylwia , Siwulski Marek , Budka Anna , Kalač Pavel , Niedzielski Przemysław , Gąsecka Monika , Mleczek Mirosław
Published: August 2022

Due to their enzymatic and bioaccumulation faculties the use of macromycetes for the decontamination of polluted matrices seems reasonable for bioremediation. For this reason, the aim of our study was to evaluate the mycoremediation ability of Agaricus bisporus cultivated on compost mixed with flotation tailings in different quantities (1, 5, 10, 15, and 20% addition). The biomass of the fruit bodies and the content of 51 major and trace elements were determined. Cultivation of A. bisporus in compost moderately polluted with flotation tailings yielded significantly lower (the first flush) and higher (the second flush) biomass of fruit bodies, compared with the control treatment. The presence of toxic trace elements did not cause any visible adverse symptoms for A. bisporus. Increasing the addition of flotation tailings to the compost induced an elevated level of most determined elements. A significant increase in rare earth elements (both flushes) and platinum group elements (first flush only) was observed. The opposite situation was recorded for major essential elements, except for Na and Mg in A. bisporus from the second flush under the most enriched compost (20%). Nevertheless, calculated bioaccumulation factor values showed a selective accumulation capacity—limited for toxic elements (except for Ag, As, and Cd) and the effective accumulation of B, Cu, K, and Se. The obtained results confirmed that A. bisporus can be used for practical application in mycoremediation in the industry although this must be preceded by larger-scale tests. This application seems to be the most favorable for media contaminated with selected elements, whose absorption by fruiting bodies is the most efficient.
A Novel Major Facilitator Superfamily Transporter Gene Aokap4 near the Kojic Acid Gene Cluster Is Involved in Growth and Kojic Acid Production in Aspergillus oryzae
Authors: Chen Tianming , Chen Ziming , Li Yuzhen , Zeng Bin , Zhang Zhe
Published: August 2022

Kojic acid is an important secondary metabolite of industrial importance produced by Aspergillus oryzae. The kojic acid gene cluster plays an essential role in kojic acid production, and harbors kojA, kojR and kojT. The deletion of kojT, encoding a major facilitator superfamily (MFS) transporter, did not completely abolish kojic acid production, implying that other transporters are required for the transport of kojic acid. The aim of this study is to look for the transporters involved in kojic acid production. Here, Aokap4 encoding a novel MFS transporter was identified, which was adjacent to kojT in the kojic acid gene cluster. The deletion of Aokap4 contributed to the hyphal growth, conidial production and biomass of A. oryzae. Moreover, Aokap4 is required for heat- and cell-wall-stress tolerance but not oxidative and osmotic stress. The disruption of Aokap4 impaired kojic acid production with the reduced expression of kojA, kojR and kojT. Furthermore, when kojT was deleted in the Aokap4-disrupted strain, the yield of kojic acid declined to the same level as that of the kojT-deletion mutant, whereas the production of kojic acid was recovered when kojT was overexpressed in the Aokap4 knockout strain, suggesting that kojT acts downstream of Aokap4. AoKap4 was the second identified MSF transporter involved in kojic acid production after kojT was found a decade ago. This study contributes to a better understanding of the biological roles of the MFS transporter and lays a foundation for future studies on kojic acid synthesis in A. oryzae.
Effect of the Applied Voltages on the Corrosion–Wear Behavior of Thermal Spray Al Coating
Authors: Jian Shun-Yi , Sheu Hung-Hua , Chang Jun-Kai , Chen Chih-Hung , Hong Yuan-Huan , Lin Ming-Hsun , Lee Hung-Bin
Published: August 2022

In this work, an Al coating prepared using the arc spray process was carried out with a corrosion–wear analysis by a block-on-ring system. The interaction of corrosion and wear of coatings in seawater was also investigated. The effect of different corrosion potentials on the corrosion and wear of the coating was discussed, and the structure and corrosion products of the coating were observed by SEM. The results of dynamic potential polarization curves and friction coefficient measurements were used to clarify the corrosion and wear behavior of aluminum coatings in seawater. After quantitative analysis of a corrosion and wear test, it was found that with the increase in polarization potential, the total weight loss of corrosion and wear (Wtotal) of the aluminum coating increased significantly. This means that the corrosion–wear interaction accounts for most of the weight loss of the coating.
Experimental Study on Effects of Aging Time on Dry Shrinkage Cracking of Lime Soils
Authors: Yue Jianwei , Chen Ying , Luo Zhenxian , Wang Siyuan , Su Huicong , Gao Huijie , Li Yuan , Li Peng , Ma Can
Published: August 2022

The effect of aging on the internal mechanism of the dry shrinkage cracking of lime soil was studied from the perspective of macroscopic cracking phenomenon and microscopic composition change, and the reasonable aging time of lime soil was determined. Large numbers of cracks often occur in buildings constructed using lime soil, which impacts sustainable development and building environmental protection. This study explored the influence of aging time on the mechanical properties and shrinkage cracking of lime soil. The influence of aging time was evaluated using a triaxial compression test; using the dry–wet cycle, sieving, pH, and other tests, the influence of aging time on volume crack rate, expansion shrinkage rate, particle size distribution, and pH was analyzed. Scanning electron microscopy and X-ray diffraction experiments were used to analyze changes in the lime soil particle structure for different aging times and the formation of new substances. The results show that as aging time increases, the stress–strain curve of the soil softens significantly, shear strength deteriorates, and cohesion decreases. When the aging time is 6 h, the expansion rate and shrinkage rate at the center of the soil sample are the maximum. The volume fracture and expansion shrinkage rates decrease first, and then plateau with aging time, with the changes remaining stable after 72 h; these rate decreases are positively correlated with the change rate of pH. The formation of Ca(OH)2 affects the sample pH, and the changes in pH, Ca(OH)2, and CaO tend to be stable. With an increase in aging time, the proportion of particles of a size less than 0.1 mm decreases, and that of particles of size 0.1–0.5 mm increases. After 72 h of aging, the particle size proportion remains unchanged. Reasonable aging time can, thus, reduce the hydration reaction of lime, improve particle agglomeration effects, and reduce the crack development of the soil.
Modeling and Prediction of Thermophysiological Comfort Properties of a Single Layer Fabric System Using Single Sector Sweating Torso
Authors: Gholamreza Farzan , Su Yang , Li Ruoyao , Nadaraja Anupama Vijaya , Gathercole Robert , Li Ri , Dolez Patricia I. , Golovin Kevin , Rossi René M. , Annaheim Simon , Milani Abbas S.
Published: August 2022

Thermophysiological comfort is known to play a primary role in maintaining thermal balance, which corresponds to a person’s satisfaction with their immediate thermal environment. Among the existing test methods, sweating torsos are one of the best tools to provide a combined measurement of heat and moisture transfer using non-isothermal conditions. This study presents a preliminary numerical model of a single sector sweating torso to predict the thermophysiological comfort properties of fabric systems. The model has been developed using COMSOL Multiphysics, based on the ISO 18640-1 standard test method and a single layer fabric system used in sportswear. A good agreement was observed between the experimental and numeral results over different exposure phases simulated by the torso test (R2 = 0.72 to 0.99). The model enables a systematic investigation of the effect of fabric properties (thickness, porosity, thermal resistance, and evaporative resistance), environmental conditions (relative humidity, air and radiant temperature, and wind speed), and physiological parameters (sweating rate) to gain an enhanced understanding of the thermophysiological comfort properties of the fabric system.
The Influence of Dimensions and Powder Recycling on the Roughness and Mechanical Properties of Ti-6Al-4V Parts Fabricated by Laser Powder Bed Fusion
Authors: Yánez Alejandro , Fiorucci María Paula , Martel Oscar , Cuadrado Alberto
Published: August 2022

Powder bed fusion technology has undergone a remarkable amount of development in recent years in the field of medical implants due to the advantages associated with it. In many implant applications that demand loads in parts with a high degree of roughness and small dimensions, the mechanical properties, especially fatigue properties, play a key role in the success of the implants. One of the most used materials in this field is Ti-6Al-4V. On the other hand, the high cost of titanium powders makes it necessary to search for suitable powder recycling strategies. In this work, the effects of dimensions and powder recycling on the roughness and the mechanical properties of cylinder specimens were obtained from tensile static and fatigue tests of Ti-6Al4V Extra-Low Interstitial (ELI) parts. Four types of specimens were fabricated by laser powder bed fusion (two dimensions (section diameters of 2 mm and 5 mm) with new powder and with recycled powder). Results show that the oxygen concentration increased with recycling. No significant effects of recycling were observed on the monotonic tensile strength specimens. However, specimens fabricated with recycled powder showed greater roughness, lower ductility, and lower fatigue strength than those fabricated with new powder. On the other hand, the 5-mm-diameter specimens showed slightly better fatigue behavior than the 2-mm-diameter ones.
Corrosive Effect of Wood Ash Produced by Biomass Combustion on Refractory Materials in a Binary Al–Si System
Authors: Ovčačíková Hana , Velička Marek , Vlček Jozef , Topinková Michaela , Klárová Miroslava , Burda Jiří
Published: August 2022

In terms of its chemical composition, biomass is a very complex type of fuel. Its combustion leads to the formation of materials such as alkaline ash and gases, and there is evidence of the corrosive effect this process has on refractory linings, thus shortening the service life of the combustion unit. This frequently encountered process is known as “alkaline oxidative bursting”. Corrosion is very complex, and it has not been completely described yet. Alkaline corrosion is the most common cause of furnace-lining degradation in aggregates that burn biomass. This article deals with an experiment investigating the corrosion resistance of 2 types of refractory materials in the Al2O3-SiO2 binary system, for the following compositions: I. (53 wt.% SiO2/42 wt.% Al2O3) and II. (28 wt.% SiO2/46 wt.% Al2O3/12 wt.% SiC). These were exposed to seven types of ash obtained from one biomass combustion company in the Czech Republic. The chemical composition of the ash is a good indicator of the problematic nature of a type of biomass. The ashes were analyzed by X-ray diffraction and X-ray fluorescence. Analysis confirmed that ash composition varies. The experiment also included the calculation of the so-called “slagging/fouling index” (I/C, TA, Sr, B/A, Fu, etc.), which can be used to estimate the probability of slag formation in combustion units. The corrosive effect on refractory materials was evaluated according to the norm ČSN P CEN/TS 15418, and a static corrosion test was used to investigate sample corrosion.
Investigating Machine Learning Applications in the Prediction of Occupational Injuries in South African National Parks
Authors: Chadyiwa Martha , Kagura Juliana , Stewart Aimee
Published: August 2022

There is a need to predict occupational injuries in South African National Parks for the purpose of implementing targeted interventions or preventive measures. Machine-learning models have the capability of predicting injuries such that the employees that are at risk of experiencing occupational injuries can be identified. Support Vector Machines (SVMs), k Nearest Neighbours (k-NN), XGB classifier and Deep Neural Networks were applied and overall performance was compared to the accuracy of baseline models that always predict low extremity injuries. Data extracted from the Department of Employment and Labour’s Compensation Fund was used for training the models. SVMs had the best performance in predicting between low extremity injuries and injuries in the torso and hands regions. However, the overall accuracy was 56%, which was slightly above the baseline and below findings from similar previous research that reported a minimum of 62%. Gender was the only feature with an importance score significantly greater than zero. There is a need to use more features related to work conditions and which acknowledge the importance of environment in order to improve the accuracy of the predictions of the models. Furthermore, more types of injuries, and employees that have not experienced any injuries, should be included in future studies.
Optimization of Open Queuing Networks with Batch Services
Authors: Stankevich Elena , Tananko Igor , Pagano Michele
Published: August 2022

In this paper, open queuing networks with Poisson arrivals and single-server infinite buffer queues are considered. Unlike traditional queuing models, customers are served (with exponential service time) in batches, so that the nodes are non-work-conserving. The main contribution of this work is the design of an efficient algorithm to find the batch sizes which minimize the average response time of the network. As preliminary steps at the basis of the proposed algorithm, an analytical expression of the average sojourn time in each node is derived, and it is shown that this function, depending on the batch size, has a single minimum. The goodness of the proposed algorithm and analytical formula were verified through a discrete-event simulation for an open network with a non-tree structure.
Potential Beneficial Effects of Sargassum spp. in Skin Aging
Authors: Lee Min-Kyeong , Ryu Heeyeon , Lee Ji Yun , Jeong Hyeon Hak , Baek Jiwon , Van Ji Yun , Kim Myeong-Jin , Jung Won-Kyo , Lee Bonggi
Published: August 2022

Seaweeds are receiving much attention as a rich source of bioactive compounds with cosmeceutical potential. Recent studies have revealed that Sargassum spp., a genus of brown algae in the family Sargassaceae, has multiple functions in preventing and improving skin aging. Sargassum spp. contains many bioactive compounds, such as fucoidan, fucoxanthin, terpenoids, flavonoids, and meroterpenoids. These Sargassum spp. extracts and derivative compounds have excellent potential for skincare, as they exhibit skin health-promoting properties, including antioxidants, anti-inflammation, whitening, skin barrier repair, and moisturizing. Therefore, searching for bioactive compounds in marine resources such as Sargassum spp. could be an attractive approach to preventing and improving skin aging. The current review focused on the various biological abilities of Sargassum extracts or derived compounds for anti-skin aging.
Does Endoscopic Transnasal Optic Nerve Decompression Followed by Radiosurgery Improve Outcomes in the Treatment of Parasellar Meningiomas?
Authors: Matoušek Petr , Cvek Jakub , Čábalová Lenka , Misiorzová Eva , Krejčí Ondřej , Lipina Radim , Krejčí Tomáš
Published: August 2022

Introduction: The clinical management of parasellar meningiomas (PM) is challenging due to their intimate association with critical neurovascular structures. Consensus regarding the recommended treatment protocol is lacking. This study will evaluate patients’ visual outcomes following endoscopic transnasal optic nerve decompression (ETOND) and will investigate the possibility of reducing the rate of complications associated with stereotactic radiosurgery (SRS). Methods: Retrospective analysis was conducted on all patients who underwent ETOND for PM between 2013 and 2020. The study comprised 12 patients (7 women and 5 men aged 36–75 years; mean, 55.2 years; median, 57.6 years) in which 14 optic nerve decompression procedures were carried out. Patients were followed up for 6 to 86 months (mean, 29.3 months; median, 25 months). There were five cases of spheno-orbital meningioma, four cases of cavernous sinus meningioma, and one case each of petro-clival meningioma, optic nerve sheath meningioma, and planum sphenoidale/tuberculum sellae meningioma. Visual outcome was evaluated and any postoperative complications noted. Results: Improvements in visual acuity were noted in 10 of 14 eyes (71.4%) 3 to 6 months postoperation. Visual acuity remained stable in the remaining four eyes. No deterioration of visual acuity was noted during the follow-up period. In total, 9 of the 12 patients underwent SRS. No tumor growth was determined, while reduction in tumor volume was noted in five patients following SRS. No complications associated with SRS or the surgical procedure were noted. Conclusions: ETOND appears to be a promising technique for increasing rates of improved visual function, while reducing the risk of post SRS-related complications. In combination with subsequent SRS, it is an ideal treatment modality in the management of parasellar meningiomas. Confirmation of our findings would require a larger, prospective multicenter study.
Incidence of Hip Fractures among Patients with Chronic Otitis Media: The Real-World Data
Authors: Liao Pei-Shao , Chiu Ching-Chih , Fu Yi-Hsiu , Hsia Chia-Chun , Yang Yu-Cih , Lee Kun-Feng , Hsieh Shang-Lin , Kuo Shu-Jui
Published: August 2022

Chronic otitis media (COM) has been considered as a localized disease, and its systemic impact is poorly understood. Whether COM-induced inflammation could be associated with systemic bone loss and hip fracture is unknown at present. Our study tried to determine the risk of hip fracture among COM patients. We selected the comparison individuals without the COM coding and paired the controls with COM patients by gender, age, and comorbidities (including osteoporosis) by about a one-to-two ratio. Our study showed that the incidence of hip fracture was 4.48 and 3.92 per 1000 person-years for comparison and COM cohorts respectively. The cumulative incidence of hip fracture is higher in the COM cohort (p < 0.001). After adjustment for gender, age, and comorbidities, the COM patients had a 1.11-fold (aHR = 1.11; 95% CI = 1.05–1.17) risk of hip fracture than the control subjects. Among COM patients, a history of hearing loss is associated with higher (aHR = 1.21; 95% CI = 1.20–1.42) fracture risk. Our study showed that COM patients, especially those with hearing loss, are susceptible to a higher risk for hip fracture.
Interactions between the Nicotinic and Endocannabinoid Receptors at the Plasma Membrane
Authors: Vallés Ana Sofía , Barrantes Francisco J.
Published: August 2022

Compartmentalization, together with transbilayer and lateral asymmetries, provide the structural foundation for functional specializations at the cell surface, including the active role of the lipid microenvironment in the modulation of membrane-bound proteins. The chemical synapse, the site where neurotransmitter-coded signals are decoded by neurotransmitter receptors, adds another layer of complexity to the plasma membrane architectural intricacy, mainly due to the need to accommodate a sizeable number of molecules in a minute subcellular compartment with dimensions barely reaching the micrometer. In this review, we discuss how nature has developed suitable adjustments to accommodate different types of membrane-bound receptors and scaffolding proteins via membrane microdomains, and how this “effort-sharing” mechanism has evolved to optimize crosstalk, separation, or coupling, where/when appropriate. We focus on a fast ligand-gated neurotransmitter receptor, the nicotinic acetylcholine receptor, and a second-messenger G-protein coupled receptor, the cannabinoid receptor, as a paradigmatic example.
Membrane Fouling and Electrochemical Regeneration at a PbO2-Reactive Electrochemical Membrane: Study on Experiment and Mechanism
Authors: Gu Liankai , Zhang Yonghao , Han Weiqing , Wei Kajia
Published: August 2022

Membrane fouling and regeneration are the key issues for the application of membrane separation (MS) technology. Reactive electrochemical membranes (REMs) exhibited high, stable permeate flux and the function of chemical-free electrochemical regeneration. This study fabricated a micro-filtration REM characterized by a PbO2 layer (PbO2-REM) to investigate the electro-triggered anti-fouling and regeneration progress within REMs. The PbO2-REM exhibited a three-dimensional porous structure with a few branch-like micro-pores. The PbO2-REM could alleviate Humic acid (HA) and Bisphenol A (BPA) fouling through electrochemical degradation combined with bubble migration, which achieved the best anti-fouling performance at current density of 4 mA cm−2 with 99.2% BPA removal. Regeneration in the electro-backwash (e-BW) mode was found as eight times that in the forward wash and full flux recovery was achieved at a current density of 3 mA cm−2. EIS and simulation study also confirmed complete regeneration by e-BW, which was ascribed to the air–water wash formed by bubble migration and flow. Repeated regeneration tests showed that PbO2-REM was stable for more than five cycles, indicating its high durability for practical uses. Mechanism analysis assisted by finite element simulation illustrated that the high catalytic PbO2 layer plays an important role in antifouling and regeneration.
Research on Measuring Pure Membrane Electrical Resistance under the Effects of Salinity Gradients and Diffusion Boundary Layer and Double Layer Resistances
Authors: Zhao Yang , Duan Liang
Published: August 2022

Forward osmosis membranes are an emerging technology with great potential applicability in energy-efficient wastewater treatments and the differentiation between two solutions. Such solutions often differ in their concentrations or compositions. In this study, the membrane electrical resistances of three different membranes, including cation or anion-exchange membranes and forward osmosis membranes, were analyzed by Luggin capillary coupled with AC impedance spectroscopy (EIS) so as to obtain the real membrane and ion transfer impedance values near the membrane interface. The results reveal that the membrane impedance obtained by both the DC and AC approaches decreased as the lowest external solution concentration increased. Furthermore, the relationship between the membrane conductivity and the internal salt solution concentration was also investigated. It can be seen that the external ion concentration is directly proportional to the free ion concentration in the membrane, and the free ion concentration in the membrane is closely related to the membrane electrical resistance.
Stability of Non-Concentric, Multilayer, and Fully Aligned Porous MoS2 Nanotubes
Authors: Peña-Obeso Pablo Jahir , Huirache-Acuña Rafael , Ramirez-Zavaleta Fernando Iguazú , Rivera José Luis
Published: August 2022

Nanotubes made of non-concentric and multiple small layers of porous MoS2 contain inner pores suitable for membrane applications. In this study, molecular dynamics simulations using reactive potentials were employed to estimate the stability of the nanotubes and how their stability compares to macroscopic single- (1L) and double-layer MoS2 flakes. The observed stability was explained in terms of several analyses that focused on the size of the area of full-covered layers, number of layers, polytype, and size of the holes in the 1L flakes. The reactive potential used in this work reproduced experimental results that have been previously reported, including the small dependency of the stability on the polytype, the formation of S–S bonds between inter- and intra-planes, and the limit of stability for two concentric rings forming a single ring-like flake.
Comparing the Phenylalanine/Tyrosine Pathway and Related Factors between Keratopathy and No-Keratopathy Groups as Well as between Genders in Alkaptonuria during Nitisinone Treatment
Authors: Ranganath Lakshminarayan R. , Milan Anna M. , Hughes Andrew T. , Davison Andrew S. , Khedr Milad , Imrich Richard , Rudebeck Mattias , Olsson Birgitta , Norman Brendan P. , Bou-Gharios George , Gallagher James A.
Published: August 2022

Nitisinone (NIT) causes tyrosinaemia and corneal keratopathy (KP), especially in men. However, the adaptation within the phenylalanine (PHE)/tyrosine (TYR) catabolic pathway during KP is not understood. The objective of this study is to assess potential differences in the PHE/TYR pathway during KP and the influence of gender in NIT-induced tyrosinaemia in alkaptonuria (AKU). Samples of serum and 24 h urine collected from patients treated with NIT during a 4-year randomized study in NIT vs. no-treatment controls (SONIA 2; Suitability Of Nitisinone In Alkaptonuria 2; EudraCT no. 2013-001633-41) at months 3 (V2), 12 (V3), 24 (V4), 36 (V5) and 48 (V6) were included in these analyses. Homogentisic acid (HGA), TYR, PHE, hydroxyphenylpyruvate (HPPA), hydroxyphenyllactate (HPLA) and sNIT were analysed at all time-points in serum and urine in the NIT-group. All statistical analyses were post hoc. Keratopathy occurred in 10 out of 69 AKU patients, eight of them male. Thirty-five sampling points (serum and 24 h urine) were analysed in patients experiencing KP and 272 in those with no-KP (NKP) during NIT therapy. The KP group had a lower HPLA/TYR ratio and a higher TYR/PHE ratio compared with the NKP group (p < 0.05 for both). There were 24, 45, 100 and 207 sampling points (serum and 24 h urine) in the NIT group which were pre-NIT female, pre-NIT male, NIT female and NIT male, respectively. The PHE/TYR ratio and the HPLA/TYR ratio were lower in males (p < 0.001 and p < 0.01, respectively). In the KP group and in the male group during NIT therapy, adaptive responses to minimise TYR formation were impaired compared to NKP group and females, respectively.
Manufacturing of Ultra-Thin X-ray-Compatible COC Microfluidic Devices for Optimal In Situ Macromolecular Crystallography Experiments
Authors: Vasireddi Ramakrishna , Gardais Antonin , Chavas Leonard
Published: August 2022

Cyclic-olefin-copolymer (COC)-based microfluidic devices are increasingly becoming the center of highly valuable research for in situ X-ray measurements due to their compatibility with X-rays, biological compounds, chemical resistance, optical properties, low cost, and simplified handling. COC microfluidic devices present potential solutions to challenging biological applications such as protein binding, folding, nucleation, growth kinetics, and structural changes. In recent years, the techniques applied to manufacturing and handling these devices have capitalized on enormous progress toward small-scale sample probing. Here, we describe the new and innovative design aspects, fabrication, and experimental implementation of low-cost and micron-sized X-ray-compatible microfluidic sample environments that address diffusion-based crystal formation for crystallographic characterization. The devices appear fully compatible with crystal growth and subsequent X-ray diffraction experiments, resulting in remarkably low background data recording. The results highlighted in this research demonstrate how the engineered microfluidic devices allow the recording of accurate crystallographic data at room temperature and structure determination at high resolution.
A Numerical Study on the Electrochemical Treatment of Chloride-Contaminated Reinforced Concrete
Authors: Xi Yanan , Gao Yun , Li Wenwei , Lei Dong
Published: August 2022

Electrochemical treatment, specified as electrochemical chloride extraction (ECE), is one of the common techniques developed for the rehabilitation of chloride-contaminated reinforced concrete. In practice, ECE is time-consuming; for instance, the treatment duration could last several weeks or even longer. In order to reduce the laboratory work, this paper presents some results about a numerical study of the ECE. It is to solve a series of physical equations governing multiple ionic transport making use of a finite difference method. The effects of some critical factors are discussed in detail, such as the treatment duration, the current density and the cover thickness. In addition, for the sake of validation, the numerical results are also compared with those obtained from an experimental test.
MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP-l-Complex-Mediated Signaling Pathway
Authors: Xu Pan , Zhang Haitong , Li Huangting , Liu Bo , Li Rongrong , Zhang Jinjin , Song Xiaodong , Lv Changjun , Li Hongbo , Chen Mingwei
Published: August 2022

Pulmonary fibrosis is characterized by the destruction of alveolar architecture and the irreversible scarring of lung parenchyma, with few therapeutic options and effective therapeutic drugs. Here, we demonstrate the anti-pulmonary fibrosis of 3-(4-methoxyphenyl)-4-oxo-4H-1-benzopyran-7-yl(αS)-α,3,4-trihydroxybenzenepropanoate (MOBT) in mice and a cell model induced by bleomycin and transforming growth factor-β1. The anti-pulmonary fibrosis of MOBT was evaluated using a MicroCT imaging system for small animals, lung function analysis and H&E and Masson staining. The results of RNA fluorescence in situ hybridization, chromatin immunoprecipitation (ChIP)-PCR, RNA immunoprecipitation, ChIP-seq, RNA-seq, and half-life experiments demonstrated the anti-pulmonary fibrotic mechanism. Mechanistic dissection showed that MOBT inhibited lncITPF transcription by preventing p-Smad2/3 translocation from the cytoplasm to the nucleus, resulting in a reduction in the amount of the lncITPF–hnRNP L complex. The decreased lncITPF–hnRNP L complex reduced MEF2c expression by blocking its alternative splicing, which in turn inhibited the expression of MEF2c target genes, such as TAGLN2 and FMN1. Briefly, MOBT alleviated pulmonary fibrosis through the lncITPF–hnRNP-l-complex-targeted MEF2c signaling pathway. We hope that this study will provide not only a new drug candidate but also a novel therapeutic drug target, which will bring new treatment strategies for pulmonary fibrosis.
CRISPR/Cas12a Coupling with Magnetic Nanoparticles and Cascaded Strand Displacement Reaction for Ultrasensitive Fluorescence Determination of Exosomal miR-21
Authors: Liu Qing , Liu Jingjian , He Na , Zhang Moli , Wu Lun , Chen Xiyu , Zhu Jun , Ran Fengying , Chen Qinhua , Zhang Hua
Published: August 2022

Exosomal MicroRNA-21 (miRNA-21, miR-21) is significantly up-regulated in blood samples of patients with lung cancer. Exosomal-derived miR-21 can be used as a promising biomarker for the early diagnosis of lung cancer. This paper develops a fluorescent biosensor based on the combination of magnetic nanoparticles (MNPs), cascade strand displacement reaction (CSDR) and CRISPR/Cas12a to detect the exosomal miR-21 from lung cancer. The powerful separation performance of MNPs can eliminate the potential interference of matrix and reduce the background signal, which is very beneficial for the improvement of specificity and sensitivity. The CSDR can specifically transform one miR-21 into plenty of DNA which can specifically trigger the trans-cleavage nuclease activity of Cas12a, resulting in the cleavage of ssDNA bi-labeled with fluorescent and a quencher. Under the optimized experimental conditions, the developed fluorescence biosensor exhibited high sensitivity and specificity towards the determination of exosomal-derived miR-21 with a linear range from 10 to 1 × 105 fM and a low detection limit of about 0.89 fM. Most importantly, this method can be successfully applied to distinguish the exosomal miR-21 from the lung cancer patients and the healthy people.
Photocatalytic Isomerization of (E)-Anethole to (Z)-Anethole
Authors: Korff Marvin , Paulisch Tiffany O. , Glorius Frank , Doltsinis Nikos L. , Wünsch Bernhard
Published: August 2022

Natural product (E)-anethole was isomerized to (Z)-anethole in a photocatalytic reaction. For this purpose, a self-designed cheap photoreactor was constructed. Among 11 photosensitizers (organo and metal complex compounds), Ir(p-tBu-ppy)3 led to the highest conversion. Triplet energies of (E)- and (Z)-anethole were predicted theoretically by DFT calculations to support the selection of appropriate photosensitizers. A catalyst loading of 0.1 mol% gave up to 90% conversion in gram scale. Further additives were not required and mild irradiation with light of 400 nm overnight was sufficient. As a proof of concept, (E)- and (Z)-anethole were dihydroxylated diastereoselectively to obtain diastereomerically pure like- and unlike-configured diols, respectively.
Phytochemical Screening, Anti-Inflammatory, and Antidiabetic Activities of Different Extracts from Caralluma edulis Plant
Authors: Khan Maria , Manzoor Zahid , Rafiq Muhammad , Munawar Shaukat Hussain , Waqas Muhammad Yasir , Majeed Hamid , Ali Shah Syed Zahid , Hussain Riaz , Hussain Hafiz Iftikhar , Tahir Tehreem , Kotwica-Mojzych Katarzyna , Mojzych Mariusz
Published: August 2022

The plant Caralluma edulis is traditionally used against diabetes and inflammatory conditions in Pakistan. This study was designed to provide scientific validation of the traditional use of Caralluma edulis. Phytochemicals were extracted from the plant by different solvents (distilled water, methanol, ethanol, and acetone) using the Soxhlet’s extraction method. Bioactive compounds were detected by gas chromatography–mass spectrometry (GC-MS). The in vitro anti-inflammatory activities (albumin denaturation, membrane stabilization, and proteinase inhibition) and antioxidant capacity (DPPH scavenging activity, FRAP reducing activity) of different extracts from Caralluma edulis were assessed. The antidiabetic potential of Caralluma edulis plant extracts was determined in acute and subacute diabetic rabbit models. Oxidative stress and enzymatic antioxidant status were also estimated in MDA, CAT, and SOD levels. Results showed that the methanol extract yielded the highest contents of phenolics, flavonoids, alkaloids, and terpenoids. The in vitro anti-inflammatory activity and antioxidant potential of the methanol extract were the highest among the tested solvents. The tested extracts did not show any remarkable antidiabetic activity in the acute diabetic model. However, all tested extracts demonstrated antidiabetic potential in the subacute diabetic model. No adverse effect was observed at the tested dose (200 mg/kg) of Caralluma edulis extracts in experimental animals. It is concluded that methanol is the key solvent for extracting bioactive compounds from Caralluma edulis. The plant can be used against inflammatory disorders and may prove a potential candidate for drug development. Long-term use of Caralluma edulis at the tested dose (200 mg/kg) showed antidiabetic properties in the animal model.
Hepatoprotective Activity of Ethanol Extract of Rice Solid-State Fermentation of Ganoderma tsugae against CCl4-Induced Acute Liver Injury in Mice
Authors: Zhang Xin , Lv Wentao , Fu Yongping , Li Yu , Wang Jinhe , Chen Dongjie , Han Xuerong , Li Zhenhao
Published: August 2022

Ganoderma tsugae is well known as a medicinal mushroom in China and many Asian countries, while its fermentation technique and corresponding pharmacological activity are rarely reported. In this study, a wild G. tsugae strain (G42) with high triterpenoid content was screened from nine strains by rice solid-state fermentation, and 53.86 mg/g triterpenoids could be produced under optimized conditions; that is, inoculation amount 20%, fermentation temperature 27 °C, and culture time 45 days. The hepatoprotective activity of G42 ethanol extract was evaluated by CCl4-induced liver injury in mice, in which changes in the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), oxidation-related factors, and inflammatory cytokines in serum or liver samples demonstrated the therapeutic effect. In addition, the ethanol extract of G42 reduced the incidence of necrosis and inflammatory infiltration, and decreased protein expression levels of phosphor-nuclear factor-κB (NF-κB), interleukin-Iβ (IL-1β), and nuclear factor erythroid-2-related factor 2 (NRF2). The chemical composition of the ethanol extract was analyzed by high-resolution mass spectrometry and molecular networking. Three main triterpenoids, namely platycodigenin, cucurbitacin IIb, and ganolecidic acid B were identified. This work provided an optimized fermentation method for G. tsugae, and demonstrated that its fermentation extract might be developed as a functional food with a hepatoprotective effect.
The Current Situation of Pea Protein and Its Application in the Food Industry
Authors: Shanthakumar Parvathy , Klepacka Joanna , Bains Aarti , Chawla Prince , Dhull Sanju Bala , Najda Agnieszka
Published: August 2022

Pea (Pisum sativum) is an important source of nutritional components and is rich in protein, starch, and fiber. Pea protein is considered a high-quality protein and a functional ingredient in the global industry due to its low allergenicity, high protein content, availability, affordability, and deriving from a sustainable crop. Moreover, pea protein has excellent functional properties such as solubility, water, and oil holding capacity, emulsion ability, gelation, and viscosity. Therefore, these functional properties make pea protein a promising ingredient in the food industry. Furthermore, several extraction techniques are used to obtain pea protein isolate and concentrate, including dry fractionation, wet fractionation, salt extraction, and mild fractionation methods. Dry fractionation is chemical-free, has no loss of native functionality, no water use, and is cost-effective, but the protein purity is comparatively low compared to wet extraction. Pea protein can be used as a food emulsifier, encapsulating material, a biodegradable natural polymer, and also in cereals, bakery, dairy, and meat products. Therefore, in this review, we detail the key properties related to extraction techniques, chemistry, and structure, functional properties, and modification techniques, along with their suitable application and health attributes.
Selenium(IV) Polybromide Complexes: Structural Diversity Driven by Halogen and Chalcogen Bonding
Authors: Korobeynikov Nikita A. , Usoltsev Andrey N. , Novikov Alexander S. , Abramov Pavel A. , Sokolov Maxim N. , Adonin Sergey A.
Published: August 2022

Reactions between bromoselenate(IV)-containing solutions, dibromine and salts of pyridinium-family organic cations resulted in structurally diverse, bromine-rich polybromine-bromoselenates(IV): (4-MePyH)5[Se2Br9][SeBr6](Br3)2 (1), (2-MePyH)2{[SeBr6](Br2)} (2), (PyH)2{[SeBr5]Br(Br2)2} (3), (1-MePy)2{[SeBr6](Br2)} (4). The compounds feature halogen and (in the case of 3) chalcogen bonding in solid state, resulting in formation of supramolecular architectures of different dimensionality. DFT calculations allowed estimation of the energies of non-covalent interactions in 1–4; additionally, characterization by Raman spectroscopy was performed.
Reduction of Weed Growth under the Influence of Extracts and Metabolites Isolated from Miconia spp.
Authors: Ximenez Gabriel Rezende , Bianchin Mirelli , Carmona João Marcos Parolo , de Oliveira Silvana Maria , Ferrarese-Filho Osvaldo , Pastorini Lindamir Hernandez
Published: August 2022

Weeds pose a problem, infesting areas and imposing competition and harvesting difficulties in agricultural systems. Studies that provide the use of alternative methods for weed control, in order to minimize negative impacts on the environment, have intensified. Native flora represents a source of unexplored metabolites with multiple applications, such as bioherbicides. Therefore, we aimed to carry out a preliminary phytochemical analysis of crude extracts and fractions of Miconia auricoma and M. ligustroides and to evaluate these and the isolated metabolites phytotoxicity on the growth of the target species. The growth bioassays were conducted with Petri dishes with lettuce, morning glory, and sourgrass seeds incubated in germination chambers. Phytochemical analysis revealed the presence of flavonoids, isolated myricetin, and a mixture of quercetin and myricetin. The results showed that seedling growth was affected in a dose-dependent manner, with the root most affected and the seedlings of the lettuce, morning glory, and sourgrass as the most sensitive species, respectively. Chloroform fractions and myricetin were the most inhibitory bioassays evaluated. The seedlings showed structural changes, such as yellowing, nonexpanded cotyledons, and less branched roots. These results indicate the phytotoxic potential of Miconia allelochemicals, since there was the appearance of abnormal seedlings and growth reduction.