bluuebunny/crossref_metadata_2025 huggingface dataset:
Deaths Attributable to Air Pollution in Nordic Countries: Disparities in the Estimates
Authors: Lehtomäki Heli , Geels Camilla , Brandt Jørgen , Rao Shilpa , Yaramenka Katarina , Åström Stefan , Andersen Mikael Skou , Frohn Lise M. , Im Ulas , Hänninen Otto
Published: May 2020

Particulate matter air pollution is widely considered as the leading environmental cause of premature mortality. However, there are substantial differences in the estimated health burden between the assessments. The aim of this work is to quantify the deaths attributable to ambient air pollution in Nordic countries applying selected assessment tools and approaches, and to identify the main disparities. We quantified and compared the estimated deaths from three health risk assessment tools and from a set of different concentration-response functions. A separate analysis was conducted for the impacts of spatial resolution of the exposure model on the estimated deaths. We found that the death rate (deaths per million) attributable to PM2.5 and O3 were the highest in Denmark and the lowest in Iceland. In the five Nordic countries, the results between the three tools ranged from 8500 to 11,400 for PM2.5 related deaths, and for ozone from 230 to 260 deaths in 2015. Substantially larger differences were found between five concentration-response functions. The shape of concentration-response functions, and applied theoretical thresholds led to substantial differences in the estimated deaths. Nordic countries are especially sensitive to theoretical thresholds due to low exposures. Sensitivity analysis demonstrated that when using spatial exposure assessment methods, high spatial resolution is necessary to avoid underestimation of exposures and health effects.
Ex-Vivo Stimulation of Adipose Stem Cells by Growth Factors and Fibrin-Hydrogel Assisted Delivery Strategies for Treating Nerve Gap-Injuries
Authors: Prautsch Katharina M. , Degrugillier Lucas , Schaefer Dirk J. , Guzman Raphael , Kalbermatten Daniel F. , Madduri Srinivas
Published: May 2020

Peripheral nerve injuries often result in lifelong disabilities despite advanced surgical interventions, indicating the urgent clinical need for effective therapies. In order to improve the potency of adipose-derived stem cells (ASC) for nerve regeneration, the present study focused primarily on ex-vivo stimulation of ASC by using growth factors, i.e., nerve growth factor (NGF) or vascular endothelial growth factor (VEGF) and secondly on fibrin-hydrogel nerve conduits (FNC) assisted ASC delivery strategies, i.e., intramural vs. intraluminal loading. ASC were stimulated by NGF or VEGF for 3 days and the resulting secretome was subsequently evaluated in an in vitro axonal outgrowth assay. For the animal study, a 10 mm sciatic nerve gap-injury was created in rats and reconstructed using FNC loaded with ASC. Secretome derived from NGF-stimulated ASC promoted significant axonal outgrowth from the DRG-explants in comparison to all other conditions. Thus, NGF-stimulated ASC were further investigated in animals and found to enhance early nerve regeneration as evidenced by the increased number of β-Tubulin III+ axons. Notably, FNC assisted intramural delivery enabled the improvement of ASC’s therapeutic efficacy in comparison to the intraluminal delivery system. Thus, ex-vivo stimulation of ASC by NGF and FNC assisted intramural delivery may offer new options for developing effective therapies.
Phage Display Selection, Identification, and Characterization of Novel Pancreatic Cancer Targeting Peptides
Authors: Asar Mallika C. , Franco April , Soendergaard Mette
Published: May 2020

Pancreatic cancer is characterized by a 5-year survival rate of 3%, in part due to inadequate detection methods. The small size of peptides offers advantages regarding molecular targeting. Thus, peptides may be used in detection of pancreatic cancer. Here, peptides that target pancreatic cancer cells were selected using phage display technology using a 15-mer fUSE5 library. Phage were pre-cleared against immortalized pancreatic cells (hTERT-HPNE), followed by selections against pancreatic cancer (Mia Paca-2) cells. Next-generation sequencing identified two peptides, MCA1 and MCA2, with a Log2 fold change (Mia Paca-2/ hTERT-HPNE) >1.5. Modified ELISA and fluorescent microscopy showed that both peptides bound significantly higher to Mia Paca-2 cells, and not to hTERT-HPNE, embryonic kidney (HEK 293), ovarian (SKOV-3) and prostate cancer (LNCaP) cell lines. Further characterization of MCA1 and MCA2 revealed EC50 values of 16.11 µM (95% CI [9.69, 26.31 µM]) and 97.01 µM (95% CI [58.64, 166.30 µM]), respectively. Based on these results, MCA1 was selected for further studies. A competitive dose response assay demonstrated specific binding and an IC50 value of 2.15 µM (95% CI [1.28, 3.62 µM]). Taken together, this study suggests that MCA1 may be used as a pancreatic cancer targeting ligand for detection of the disease.
One-Pot Alcoholysis of the Lignocellulosic Eucalyptus nitens Biomass to n-Butyl Levulinate, a Valuable Additive for Diesel Motor Fuel
Authors: Antonetti Claudia , Gori Samuele , Licursi Domenico , Pasini Gianluca , Frigo Stefano , López Mar , Parajó Juan Carlos , Raspolli Galletti Anna Maria
Published: May 2020

The present investigation represents a concrete example of complete valorization of Eucalyptus nitens biomass, in the framework of the circular economy. Autohydrolyzed-delignified Eucalyptus nitens was employed as a cheap cellulose-rich feedstock in the direct alcoholysis to n-butyl levulinate, adopting n-butanol as green reagent/reaction medium, very dilute sulfuric acid as a homogeneous catalyst, and different heating systems. The effect of the main reaction parameters to give n-butyl levulinate was investigated to check the feasibility of this reaction and identify the coarse ranges of the main operating variables of greater relevance. High n-butyl levulinate molar yields (35–40 mol%) were achieved under microwave and traditional heating, even using a very high biomass loading (20 wt%), an eligible aspect from the perspective of the high gravity approach. The possibility of reprocessing the reaction mixture deriving from the optimized experiment by the addition of fresh biomass was evaluated, achieving the maximum n-butyl levulinate concentration of about 85 g/L after only one microwave reprocessing of the mother liquor, the highest value hitherto reported starting from real biomass. The alcoholysis reaction was further optimized by Response Surface Methodology, setting a Face-Centered Central Composite Design, which was experimentally validated at the optimal operating conditions for the n-butyl levulinate production. Finally, a preliminary study of diesel engine performances and emissions for a model mixture with analogous composition to that produced from the butanolysis reaction was performed, confirming its potential application as an additive for diesel fuel, without separation of each component.
Abatement of Toluene by Reverse-Flow Nonthermal Plasma Reactor Coupled with Catalyst
Authors: Liang Wenjun , Sun Huipin , Shi Xiujuan , Zhu Yuxue
Published: May 2020

In order to make full use of the heat in nonthermal plasma systems and decrease the generation of by-products, a reverse-flow nonthermal plasma reactor coupled with catalyst was used for the abatement of toluene. In this study, the toluene degradation performance of different reactors was compared under the same conditions. The mechanism of toluene abatement by nonthermal plasma coupled with catalyst was explored, combined with the generation of ozone (O3), NO2, and organic by-products during the reaction process. It was found that a long reverse cycle time of the reactor and a short residence time of toluene decreased the internal reactor temperature, which was not beneficial for the degradation of toluene. Compared with the dielectric barrier discharge (DBD) reactor, toluene degradation efficiency in the double dielectric barrier discharge (DDBD) reactor was improved at the same discharge energy level, but the concentrations of NO2 and O3 in the effluent were relatively high; this was improved after the introduction of a catalyst. In the reverse-flow nonthermal plasma reactor coupled with catalyst, the CO2 selectivity was the highest, while the selectivity and amount of NO2 was the lowest and aromatics, acids, and ketones were the main gaseous organic by-products in the effluent. The reverse-flow DBD-catalyst reactor was successful in decreasing organic by-products, while the types of organic by-products in the DDBD reactor were much more than those in the DBD reactor.
Regulation of TMEM16A by CK2 and Its Role in Cellular Proliferation
Authors: Pinto Madalena C. , Schreiber Rainer , Lerias Joana , Ousingsawat Jiraporn , Duarte Aires , Amaral Margarida , Kunzelmann Karl
Published: May 2020

Casein kinase 2 (CK2) is a highly ubiquitous and conserved serine/threonine kinase that forms a tetramer consisting of a catalytic subunit (CK2α) and a regulatory subunit (CK2β). Despite being ubiquitous, CK2 is commonly found at higher expression levels in cancer cells, where it inhibits apoptosis, and supports cell migration and proliferation. The Ca2+-activated chloride channel TMEM16A shows similar effects in cancer cells: TMEM16A increases cell proliferation and migration and is highly expressed in squamous cell carcinoma of the head and neck (HNSCC) as well as other malignant tumors. A microscopy-based high-throughput screening was performed to identify proteins that regulate TMEM16A. Within this screen, CK2 was found to be required for proper membrane expression of TMEM16A. small interfering (si) RNA-knockdown of CK2 reduced plasma membrane expression of TMEM16A and inhibited TMEM16A whole cell currents in (cystic fibrosis bronchial epithelial) CFBE airway epithelial cells and in the head and neck cancer cell lines Cal33 and BHY. Inhibitors of CK2, such as TBB and the preclinical compound CX4549 (silmitasertib), also blocked membrane expression of TMEM16A and Ca2+-activated whole cell currents. siRNA-knockout of CK2 and its pharmacological inhibition, as well as knockdown or inhibition of TMEM16A by either niclosamide or Ani9, attenuated cell proliferation. Simultaneous inhibition of CK2 and TMEM16A strongly potentiated inhibition of cell proliferation. Although membrane expression of TMEM16A is reduced by inhibition of CK2, our data suggest that the antiproliferative effects by inhibition of CK2 are mostly independent of TMEM16A. Simultaneous inhibition of TMEM16A by niclosamide and inhibition of CK2 by silmitasertib was additive with respect to blocking cell proliferation, while cytotoxicity was reduced when compared to solely blockade of CK2. Therefore, parallel blockade TMEM16A by niclosamide may assist with anticancer therapy by silmitasertib.
Reactive Sterol Electrophiles: Mechanisms of Formation and Reactions with Proteins and Amino Acid Nucleophiles
Authors: Porter Ned A. , Xu Libin , Pratt Derek A.
Published: May 2020

Radical-mediated lipid oxidation and the formation of lipid hydroperoxides has been a focal point in the investigation of a number of human pathologies. Lipid peroxidation has long been linked to the inflammatory response and more recently, has been identified as the central tenet of the oxidative cell death mechanism known as ferroptosis. The formation of lipid electrophile-protein adducts has been associated with many of the disorders that involve perturbations of the cellular redox status, but the identities of adducted proteins and the effects of adduction on protein function are mostly unknown. Both cholesterol and 7-dehydrocholesterol (7-DHC), which is the immediate biosynthetic precursor to cholesterol, are oxidizable by species such as ozone and oxygen-centered free radicals. Product mixtures from radical chain processes are particularly complex, with recent studies having expanded the sets of electrophilic compounds formed. Here, we describe recent developments related to the formation of sterol-derived electrophiles and the adduction of these electrophiles to proteins. A framework for understanding sterol peroxidation mechanisms, which has significantly advanced in recent years, as well as the methods for the study of sterol electrophile-protein adduction, are presented in this review.
Deformation Induced Soft and Hard Lath Packets Enhance Ductility in Martensitic Steels
Authors: Ódor Éva , Jóni Bertalan , Ribárik Gábor , Chinh Nguyen Quang , Ungár Tamás , Szabó Péter J.
Published: May 2020

Martensitic steels are widely used structural materials with outstanding mechanical properties. Their high strength is provided by the non-diffusional phase transformation of fcc γ into thin lamellar bcc plates during fast cooling. Coherency strains between the fcc and bcc lamellae induce large dislocation densities in the range of 1016 m−2, well above the densities attainable by conventional plastic deformation. Using high resolution X-ray line profile analysis, scanning electron microscopy, and hardness tests we show that during tensile deformation when the active Burgers vectors are within the lath plane the lath-packets work soften. On the contrary, when the active Burgers vectors are oblique to the lath-plane the lath-packets work harden. The softening and hardening processes in the differently oriented lath-packets produce a composite of hard and soft components on the length scale of lath-packet size. The stress–strain response of the alloy is discussed in terms of the different mean free paths and the different annihilation lengths of dislocations in the softened and hardened lath-packets. The relatively good ductility is shown to be produced by the composite microstructure induced by plastic strain.
Loss of Integrase Interactor 1 (INI1) Expression in a Subset of Differentiated Thyroid Cancer
Authors: Ho Kung-Chen , Lee Jie-Jen , Lin Chi-Hsin , Leung Ching-Hsiang , Cheng Shih-Ping
Published: May 2020

Alterations in the switching defective/sucrose non-fermenting (SWI/SNF) chromatin-remodeling complex are enriched in advanced thyroid cancer. Integrase interactor 1 (INI1), encoded by the SMARCB1 gene on the long arm of chromosome 22, is one of the core subunits of the SWI/SNF complex. INI1 immunohistochemistry is frequently used for the diagnosis of malignant rhabdoid neoplasms. In the present study, we found normal and benign thyroid tissues generally had diffusely intense nuclear immunostaining. Loss of INI1 immunohistochemical expression was observed in 8% of papillary thyroid cancer and 30% of follicular thyroid cancer. Furthermore, loss of INI1 expression was associated with extrathyroidal extension (p < 0.001) and lymph node metastasis (p = 0.038). Analysis of The Cancer Genome Atlas database revealed that SMARCB1 underexpression was associated with the follicular variant subtype and aneuploidy in papillary thyroid cancer. We speculate that SMARCB1 is an important effector in addition to NF2 and CHEK2 inactivation among thyroid cancers with chromosome 22q loss.
Thermodynamics of the Coarse-Graining Master Equation
Authors: Schaller Gernot , Ablaßmayer Julian
Published: May 2020

We study the coarse-graining approach to derive a generator for the evolution of an open quantum system over a finite time interval. The approach does not require a secular approximation but nevertheless generally leads to a Lindblad–Gorini–Kossakowski–Sudarshan generator. By combining the formalism with full counting statistics, we can demonstrate a consistent thermodynamic framework, once the switching work required for the coupling and decoupling with the reservoir is included. Particularly, we can write the second law in standard form, with the only difference that heat currents must be defined with respect to the reservoir. We exemplify our findings with simple but pedagogical examples.
High-Capacity Dual-Electrolyte Aluminum–Air Battery with Circulating Methanol Anolyte
Authors: Teabnamang Pemika , Kao-ian Wathanyu , Nguyen Mai Thanh , Yonezawa Tetsu , Cheacharoen Rongrong , Kheawhom Soorathep
Published: May 2020

Aluminum–air batteries (AABs) have recently received extensive attention because of their high energy density and low cost. Nevertheless, a critical issue limiting their practical application is corrosion of aluminum (Al) anode in an alkaline aqueous electrolyte, which results from hydrogen evolution reaction (HER). To effectively solve the corrosion issue, dissolution of Al anode should be carried out in a nonaqueous electrolyte. However, the main cathodic reaction, known as oxygen reduction reaction (ORR), is sluggish in such a nonaqueous electrolyte. A dual-electrolyte configuration with an anion exchange membrane separator allows AABs to implement a nonaqueous anolyte along with an aqueous catholyte. Thus, this work addresses the issue of anode corrosion in an alkaline Al–air flow battery via a dual-electrolyte system. The battery configuration consisted of an Al anode | anolyte | anion exchange membrane | catholyte | air cathode. The anolytes were methanol solutions containing 3 M potassium hydroxide (KOH) with different ratios of water. An aqueous polymer gel electrolyte was used as the catholyte. The corrosion of Al in the anolytes was duly investigated. The increase of water content in the anolyte reduced overpotential and exhibited faster anodic dissolution kinetics. This led to higher HER, along with a greater corrosion rate. The performance of the battery was also examined. At a discharge current density of 10 mA·cm−2, the battery using the anolyte without water exhibited the highest specific capacity of 2328 mAh/gAl, producing 78% utilization of Al. At a higher content of water, a higher discharge voltage was attained. However, due to greater HER, the specific capacity of the battery decreased. Besides, the circulation rate of the anolyte affected the performance of the battery. For instance, at a higher circulation rate, a higher discharge voltage was attained. Overall, the dual-electrolyte system proved to be an effective approach for suppressing anodic corrosion in an alkaline Al–air flow battery and enhancing discharge capacity.
Macroscopic Spray Behavior of a Single-Hole Common Rail Diesel Injector Using Gasoline-Blended 5% Biodiesel
Authors: Thongchai Sakda , Lim Ocktaeck
Published: May 2020

This research studies the macroscopic spray structure from a single-hole common rail diesel injector using gasoline-blended 5% biodiesel for use in compression ignition engines. To reduce the NOX/PM trade-off emissions, researchers are investigating gasoline used in compression ignition engines, called gasoline compression ignition. As a result that gasoline is injected directly into the combustion chamber, its spray field has a significant effect on combustion and emissions. Due to its low lubricity, gasoline is blended with biodiesel 5%, as a lubricity enhancer, to prevent the failure of the high-pressure injection system. The macroscopic spray structures of this gasoline blend were investigated Schlieren photography and planar laser-induced fluorescence-particle image velocimetry. Injection pressure was handled by a conventional common rail system, while ambient pressure was controlled by supplying nitrogen into the constant-volume combustion chamber. The effects of injection pressure and ambient pressure on the gasoline spray elucidated by Planar laser-induced fluorescence coupled with particle-image velocimetry (PLIF-PIV) imagery and comparisons with variations in neat diesel spray. In addition, the flow field of gasoline spray that formed vortexes and vorticity was characterized. The results show that the injection pressure and back pressure had the same effects on the gasoline spray structure, in terms of the penetration tip and cone angle, as on the diesel spray. However, the injection pressure had a greater effect on the diesel spray than the gasoline at low ambient pressure due to the occurrence of cavitation. Moreover, the images show the remarkable turbulent structure of gasoline spray and indicate air entrainment at the spray tip region.
Remote Laboratory Testing Demonstration
Authors: Pellegrino Luigi , Sandroni Carlo , Bionda Enea , Pala Daniele , Lagos Dimitris T. , Hatziargyriou Nikos , Akroud Nabil
Published: May 2020

The complexity of a smart grid with a high share of renewable energy resources introduces several issues in testing power equipment and controls. In this context, real-time simulation and Hardware in the Loop (HIL) techniques can tackle these problems that are typical for power system testing. However, implementing a convoluted HIL setup in a single infrastructure can be physically impossible or can increase the time required to test a smart grid application in detail. This paper introduces the Joint Test Facility for Smart Energy Networks with Distributed Energy Resources (JaNDER) that allows users to exchange data in real-time between two or more infrastructures. This tool enables the integration of infrastructures, exploiting the synergies between them, and creating a virtual infrastructure that can perform more experiments using a combination of the resources installed in each infrastructure. In particular, JaNDER can extend a HIL setup. In order to validate this new testing concept, a coordinated voltage controller has been tested in a Controller HIL setup where JaNDER was used to interact with an actual On Load Tap Changer (OLTC) controller located in a remote infrastructure. The results show that the latency introduced by JaNDER is not critical; hence, under certain circumstances, it can be used to expand the real-time testing without affecting the stability of the experiment.
Optimal Location-Reallocation of Battery Energy Storage Systems in DC Microgrids
Authors: Montoya Oscar Danilo , Gil-González Walter , Rivas-Trujillo Edwin
Published: May 2020

This paper deals with the problem of optimal location and reallocation of battery energy storage systems (BESS) in direct current (dc) microgrids with constant power loads. The optimization model that represents this problem is formulated with two objective functions. The first model corresponds to the minimization of the total daily cost of buying energy in the spot market by conventional generators and the second to the minimization of the costs of the daily energy losses in all branches of the network. Both the models are constrained by classical nonlinear power flow equations, distributed generation capabilities, and voltage regulation, among others. These formulations generate a nonlinear mixed-integer programming (MINLP) model that requires special methods to be solved. A dc microgrid composed of 21-nodes with existing BESS is used for validating the proposed mathematical formula. This system allows to identify the optimal location or reallocation points for these batteries by improving the daily operative costs regarding the base cases. All the simulations are conducted via the general algebraic modeling system, widely known as the General Algebraic Modeling System (GAMS).
Optimization of a Small Wind Turbine for a Rural Area: A Case Study of Deniliquin, New South Wales, Australia
Authors: Khlaifat Nour , Altaee Ali , Zhou John , Huang Yuhan , Braytee Ali
Published: May 2020

The performance of a wind turbine is affected by wind conditions and blade shape. This study aimed to optimize the performance of a 20 kW horizontal-axis wind turbine (HAWT) under local wind conditions at Deniliquin, New South Wales, Australia. Ansys Fluent (version 18.2, Canonsburg, PA, USA) was used to investigate the aerodynamic performance of the HAWT. The effects of four Reynolds-averaged Navier–Stokes turbulence models on predicting the flows under separation condition were examined. The transition SST model had the best agreement with the NREL CER data. Then, the aerodynamic shape of the rotor was optimized to maximize the annual energy production (AEP) in the Deniliquin region. Statistical wind analysis was applied to define the Weibull function and scale parameters which were 2.096 and 5.042 m/s, respectively. The HARP_Opt (National Renewable Energy Laboratory, Golden, CO, USA) was enhanced with design variables concerning the shape of the blade, rated rotational speed, and pitch angle. The pitch angle remained at 0° while the rising wind speed improved rotor speed to 148.4482 rpm at rated speed. This optimization improved the AEP rate by 9.068% when compared to the original NREL design.
Sedimentary and Diagenetic Features and Their Impacts on Microbial Carbonate Reservoirs in the Fourth Member of the Middle Triassic Leikoupo Formation, Western Sichuan Basin, China
Authors: Wang Yuanchong , Jiang Weimin , Liu Hangyu , Liu Bo , Zheng Haofu , Song Xiaobo , Wang Qiongxian , Wang Wenkai , Li Yong
Published: May 2020

In recent years, the discovery of two gas fields in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China confirmed the exploration potential of microbial carbonates. The aim of the present study is to clarify the formation mechanism of the microbial reservoirs in the Leikoupo Formation. For this purpose, lithofacies, depositional environments, and diagenesis analyses were performed in samples collected from cores of 12 wells. The climate of study area was arid during Anisian time, and the water body was restricted. In such a climate, an evaporitic environment was developed, where ten types of lithofacies, dominated by microbial carbonates and gypsum rocks, were recognized. Thrombolites and stromatolites are the main high-quality reservoirs rock types in the fourth member of the Leikoupo Formation in the Western Sichuan Basin of SW China, which developed as microbial mounds, with reservoir space of microbial inter-clot pores, intra-clot pores, fenestral pores, inter-crystalline pores, and cracks. The microbial inter-clot pores are the main reservoir space, formed by trapping and binding of marls by benthic microbial communities. These pores were partially filled with evaporites because of the arid climate, which were subsequently dissolved (mainly gypsum) in the syn-depositional period, thus greatly improving the quality of reservoirs. Although some pores were occluded by multi-stage cements during the burial stage, major pores were well preserved own to the early dolomitization, rapid burial of the Leikoupo Formation, and early charging of hydrocarbon. The early dolomitization enhanced the anti-compaction ability of microbial carbonates during the burial stage. Rapid burial of the Leikoupo succession slowed down early cementation, and it also accelerated the maturation and expulsion process source rock to promote early charging of hydrocarbon in pores, which created a closed system, inhibiting strong burial cementation.
Planning Under Uncertainty Applications in Power Plants Using Factored Markov Decision Processes
Authors: Reyes Alberto , Sucar L. Enrique , Ibargüengoytia Pablo H. , Morales Eduardo F.
Published: May 2020

Due to its ability to deal with non-determinism and partial observability, represent goals as an immediate reward function and find optimal solutions, planning under uncertainty using factored Markov Decision Processes (FMDPs) has increased its importance and usage in power plants and power systems. In this paper, three different applications using this approach are described: (i) optimal dam management in hydroelectric power plants, (ii) inspection and surveillance in electric substations, and (iii) optimization of steam generation in a combined cycle power plant. For each case, the technique has demonstrated to find optimal action policies in uncertain settings, present good response and compilation times, deal with stochastic variables and be a good alternative to traditional control systems. The main contributions of this work are as follows, a methodology to approximate a decision model using machine learning techniques, and examples of how to specify and solve problems in the electric power domain in terms of a FMDP.
Co-Pyrolysis of Beet Pulp and Defecation Lime in TG-MS System
Authors: Slezak Radosław , Krzystek Liliana , Dziugan Piotr , Ledakowicz Stanisław
Published: May 2020

The process of pyrolysis of beet pulp, a by-product after the extraction of raw sugar from sugar beet, with the addition of defecation lime was studied in a thermobalance coupled with a mass spectrometer. The beet pulp pyrolysis process took place completely at 600 °C, and the resulting char, tar and gas were characterized by higher heating values of 23.9, 21.6 and 7.77 MJ/kg, respectively. The addition of the defecation lime to beet pulp caused both an increase in the char production yield and a decrease in the tar production yield. At the same time, the higher heating value of char and tar decreased along with the increase of defecation lime added to the sample. The deconvolution of derivative thermogravimetric (DTG) curves allowed us to identify the basic components of beet pulp, for which the activation energy by isoconversion method was calculated. The 20 wt.% addition of defecation lime caused an increase of the activation energy by about 18%. Further increase in the defecation lime content resulted in a reduction of activation energy. At the temperature above 600 °C, calcination of calcium carbonate contained in defecation lime occurred. The CO2 produced during calcination process did not cause auto-gasification of char.
Transient Behavior of Vertical Commingled Well in Vertical Non-Uniform Boundary Radii Reservoir
Authors: Sun Bing , Shi Wenyang , Zhang Rui , Cheng Shiqing , Zhang Chengwei , Di Shiying , Cui Nan
Published: May 2020

Transient behavior analysis, including rate transient analysis (RTA) and pressure transient analysis (PTA), is a powerful tool to investigate the production performance of the vertical commingled well from long-term production data and capture the formation parameters of the multilayer reservoir from transient well testing data. Current transient behavior analysis models hardly consider the effect of vertical non-uniform boundary radii (VNBR) on transient performances (rate decline and pressure response). The VNBR may cause an obvious novel radial flow regime and rate decline type, which can easily be mistaken as a radial composite reservoir. In this paper, we present a VNBR transient behavior analysis model, the extended vertical uniform boundary radii (VUBR), to investigate the rate decline behavior and pressure response characteristics through diagnostic type curves. Results indicate that the dimensionless production integral derivative curve or pressure derivative curve can magnify the differences so that we can diagnose the outer-convex shape and size of the VNBR. Therefore, it is significant to incorporate the effects of VNBR into the transient behavior analysis models of the vertical commingled well, and the model proposed in this paper enables us to better evaluate well performance, capture formation characteristics and diagnose flow regimes based on rate/pressure transient data.
Virtual Storage-Based Model for Estimation of Economic Benefits of Electric Vehicles in Renewable Portfolios
Authors: Vasilj Josip , Jakus Damir , Sarajcev Petar
Published: May 2020

The expected increase in the presence of electric vehicles raises numerous questions regarding their impact on the market relations. Depending on the agreement between the involved parties, the position of EVs changes from passive (traditional role) to active. Active EVs are beneficial for variability and uncertainty-intense modern power systems. To enable this transition, a suitable framework in the form of agreements is required in order to establish the terms and responsibilities. Following the presented agreements, we propose a novel method for evaluation of the benefits that the newly added EVs bring to the portfolio. The method comprises two steps, a Monte Carlo simulation of the EV driving/charging patterns and an optimization model for market related decision making. The method results in the estimates on economic savings resulting from adding EVs to portfolios. An illustrative example is used in order to give an idea of the range of the benefits.
The Microclimate Design Process in Current African Development: The UEM Campus in Maputo, Mozambique
Authors: Chiri Giovanni M. , Achenza Maddalena , Canì Anselmo , Neves Leonardo , Tendas Luca , Ferrari Simone
Published: May 2020

Even if current action towards sustainability in architecture mainly concerns single buildings, the responsibility of the urban shape on local microclimate has largely been ascertained. In fact, it heavily affects the energy performances of the buildings and their environmental behaviour. This produces the necessity to broaden the field of intervention toward the urban scale, involving in the process different disciplines, from architecture to fluid dynamics and physics. Following these ideas, the Masterplan for the Campus of the University Eduardo Mondlane in Maputo (Mozambique) develops a methodology that integrates microclimatic data and analyses from the initial design model. The already validated software ENVI-met (Version 4.4, ENVI_MET GmbH, Essen, Germany) acts as a useful ‘feedback’ tool that is able to assess the microclimatic behaviour of the design concept, also in terms of outdoor comfort. In particular, the analysis focused on the microclimatic performances of a ‘C’ block typology east oriented in relation to the existing buildings, in Maputo’s specific climatic characteristics. The initial urban proposal was gradually evaluated and modified in relation to the main critical aspects highlighted by the microclimatic analyses, in a sort of circular process that ended with a proposed solution ensuring better outdoor comfort than the existing buildings, and which provided an acceptable balance between spatial and climatic instances.
The Effect of Whey on Performance, Gut Health and Bone Morphology Parameters in Broiler Chicks
Authors: Tsiouris Vasileios , Kontominas Michael G. , Filioussis Giorgos , Chalvatzi Sofia , Giannenas Ilias , Papadopoulos Georgios , Koutoulis Konstantinos , Fortomaris Paschalis , Georgopoulou Ioanna
Published: May 2020

Whey is a highly nutritious byproduct of the cheese industry that can be used effectively in the animal feed industry. However, the use of whey in poultry diets is limited by its high lactose and mineral contents. The objective of the present study was to evaluate the effect of different concentrations of whey in poultry diets on the performance, intestinal microbiota and physico-chemical parameters of the intestinal ecosystem, as well as on the bone morphology and its strength in broiler chicks. One hundred and twenty-eight, day-old, male broiler chicks were randomly allocated into four treatment groups of 32 chicks each. The treatment groups were: group A, which served as negative control and groups B, C and D, supplemented with 1, 2 and 5% of dietary whey, respectively. Performance of the groups was evaluated throughout the experiment. Following necropsies, the gastrointestinal tract from each bird was removed, divided into its anatomical parts and intestinal samples were taken for microbiological analysis and for pH and viscosity measurement as well. Tibiotarsus was also collected for morphometric analysis and strength evaluation. The statistical analysis of the experimental data revealed that the dietary supplementation of 1 and 2% of whey improved significantly (p ≤ 0.05) the body weight, while the addition of 5% of whey reduced significantly (p ≤ 0.05) the body weight. Furthermore, the addition of 1, 2 and 5% of dietary whey increased significantly (p ≤ 0.05) the pH of jejunum digesta and reduced significantly (p ≤ 0.05) the pH of caecum digesta compared to the control group. The addition of 1 and 2% of whey reduced significantly (p ≤ 0.05) the viscosity in the jejunum and ileum digesta, compared to the addition of 5% of whey which reduced significantly (p ≤ 0.05) the viscosity in jejunum digesta but increased significantly (p ≤ 0.05) the viscosity in ileum digesta. Moreover, the addition of 1, 2 and 5% of dietary whey increased significantly (p ≤ 0.05) the caecal counts of Lactobacillus spp. and Lactococcus lactis, while the addition of 5% of whey reduced significantly (p ≤ 0.05) the tibiotarsus length. It can be concluded that the addition of low quantities of whey up to 2% promoted the performance and gut health of birds, while the addition of higher quantities of whey at the level of 5% had a detrimental effect on the performance and tibiotarsus length.
Simplified Mathematical Model for the Description of Anomalous Migration of Soluble Substances in Vertical Filtration Flow
Authors: Bohaienko Vsevolod , Bulavatsky Volodymyr
Published: May 2020

Since the use of the fractional-differential mathematical model of anomalous geomigration process based on the MIM (mobile–immoble media) approach in engineering practice significantly complicates simulations, a corresponding simplified mathematical model is constructed. For this model, we state a two-dimensional initial-boundary value problem of convective diffusion of soluble substances under the conditions of vertical steady-state filtration of groundwater with free surface from a reservoir to a coastal drain. To simplify the domain of simulation, we use the technique of transition into the domain of complex flow potential through a conformal mapping. In the case of averaging filtration velocity over the domain of complex flow potential, an analytical solution of the considered problem is obtained. In the general case of a variable filtration velocity, an algorithm has been developed to obtain numerical solutions. The results of process simulation using the presented algorithm shows that the constructed mathematical model can be efficiently used to simplify and accelerate modeling process.
Quaternary Thrusting in the Central Oman Mountains—Novel Observations and Causes: Insights from Optical Stimulate Luminescence Dating and Kinematic Fault Analyses
Authors: Moraetis Daniel , Scharf Andreas , Mattern Frank , Pavlopoulos Kosmas , Forman Steven
Published: May 2020

For the first time, Quaternary thrusts are documented within the Central Oman Mountains to the northwest of the Jabal Akhdar Dome. Thrusts with a throw of up to 1.1 m displace Quaternary alluvial fan conglomerates. These conglomerates have an Optical Stimulate Luminescence (OSL) age of 159 ± 7.9 ka BP and were deposited during MIS 6 (Marine Isotope Stage). The thrusts occur in two sets. Sets 1 and 2 formed during NE/SW and NW/SE shortening, respectively. Set-1-thusts correlate with the present-day stress field of NE/SW shortening which is related to subduction in the Makran Subduction Zone, and they strike parallel to the main continuous fold axis of the Jabal Akhdar and Hawasina windows. Set-2-thrusts correspond to NW/SE shortening and Plio-Pleistocene contractional structures in the southwestern Jabal Akhdar Dome. Set-2-thrusts are probably related to local variations of the present-day stress field originating from the Musandam area which is a part of the Zagros Collision Zone. Both thrust sets mimic the main thrust directions (NW/SE and NE/SW) within the Permo-Mesozoic allochthonous units (Semail Ophiolite, Hawasina napps) of the larger study area. The investigated thrusts imply some reactivation of the Hawasina and Semail thrusts due to far-field stress either from the Makran Subduction Zone and/or the Zagros Collision Zone. The ongoing tectonic activity of this part of the Oman Mountains, which has been considered of moderate activity, is for first time identified by structural data as contractional.
Spatial-Temporal Dynamics of the Ephemeral Gully Belt on the Plowed Slopes of River Basins in Natural and Anthropogenic Landscapes of the East of the Russian Plain
Authors: Yermolayev Oleg , Platoncheva Evgeniya , Essuman-Quainoo Benedict
Published: May 2020

Erosion is the leading process of soil degradation on agricultural land. In the spectrum of erosion processes, the most unfavorable for soil degradation are the processes of linear (ephemeral and gully) erosion. An assessment of the dynamics of linear erosion in the intensive farming zone of the European part of Russia (EPR) is relevant due to the lack of generalized data on the development of this type of erosion in the post-Soviet period and also, due to the highest intensity of soil erosion in the ephemeral gully erosion. The development of information technologies and the availability of high-resolution and ultra-high-resolution satellite images make it possible to solve the problems of ephemeral gully erosion belts identification, and also makes it possible to trace the dynamics of development of stream erosion on arable lands over a period characterized by the greatest changes in the climate system and economic conditions in the post-Soviet period (1980s–2010s). The study was conducted on the eastern wing of the boreal ecotone of the Russian Plain within the southern border of these zones of mixed and broad-leaved forests, forest-steppe, and steppe landscapes using the basin approach. For the initial material, satellite images of medium (30 m) and high resolution (0.5–1.5 m) were used in the work. The study used methods of image interpretation such as remote sensing of the earth and geoinformation mapping. For 70 key areas (interfluve spaces of river basins), the study developed a method of geoinformation mapping of the ephemeral gully erosion belt dynamics on arable lands. In the same way, the research developed a system of quantitative indicators characterizing its development on arable slopes. The dynamics of ephemeral gully erosion was evaluated over three-time intervals: the 1980s, 2000s, and 2010s by determining the horizontal dissection (density) and density of ephemeral gully erosion. Over the past 30 years, in the direction from the south of the forest sub-zone to the forest-steppe and steppe landscapes, there was a sharp increase in the horizontal dissection and density of the ephemeral gully network: an average of 4.6 and 10 times, respectively. The ephemeral gully erosion belt advances toward the watershed because of the formation of new erosion in the upper parts of the ephemeral gully networks and its extension, while there is a noticeable reduction in the width of the erosion-weakly active belt-sheet and rill erosion.
Novel Antibodies Targeting MUC1-C Showed Anti-Metastasis and Growth-Inhibitory Effects on Human Breast Cancer Cells
Authors: Kim Min Jung , Choi Jong Rip , Tae Nara , Wi Tae Min , Kim Kristine M. , Kim Dae Hee , Lee Eung Suk
Published: May 2020

Mucin1 (MUC1) is aberrantly glycosylated and overexpressed in various cancers, and it plays a crucial role in cancerogenesis. MUC1 is a type I membranous protein composed of α and β subunits. MUC1-α can be cleaved in cancers, exposing MUC1-β (MUC1-C). MUC1-C is involved with multiple cancer cellular functions, which makes it an attractive target for cancer treatment. However, its multifunctional mechanisms have not been fully elucidated and there has not been a successful therapeutic development against MUC1-C. Through a phage display process, we isolated the specific antibodies for the extracellular domain of MUC1-C. The relevant full IgG antibodies were produced successfully from mammalian cells and validated for their MUC1-C specificities through ELISA, dual FACS analysis, BLI assay, and confocal image analysis. In the comparison with reference antibody, elected antibodies showed characteristic bindings on target antigens. In the functionality assessment of high-ranking antibodies, SKM1-02, -13, and -20 antibodies highly inhibited invasion by triple-negative breast cancer (TNBC) cells and the SKM1-02 showed strong growth inhibition of cancer cells. Our results showed that these MUC1-C specific antibodies will be important tools for the understanding of MUC1 oncogenesis and are also highly effective therapeutic candidates against human breast cancers, especially TNBC cells.
Whole-Genome Deep Sequencing Reveals Host-Driven in-planta Evolution of Columnea Latent Viroid (CLVd) Quasi-Species Populations
Authors: Tangkanchanapas Parichate , Haegeman Annelies , Ruttink Tom , Höfte Monica , De Jonghe Kris
Published: May 2020

Columnea latent viroid (CLVd) is one of the most serious tomato diseases. In general, viroids have high mutation rates. This generates a population of variants (so-called quasi-species) that co-exist in their host and exhibit a huge level of genetic diversity. To study the population of CLVd in individual host plants, we used amplicon sequencing using specific CLVd primers linked with a sample-specific index sequence to amplify libraries. An infectious clone of a CLVd isolate Chaipayon-1 was inoculated on different solanaceous host plants. Six replicates of the amplicon sequencing results showed very high reproducibility. On average, we obtained 133,449 CLVd reads per PCR-replicate and 79 to 561 viroid sequence variants, depending on the plant species. We identified 19 major variants (>1.0% mean relative abundance) in which a total of 16 single-nucleotide polymorphisms (SNPs) and two single nucleotide insertions were observed. All major variants contained a combination of 4 to 6 SNPs. Secondary structure prediction clustered all major variants into a tomato/bolo maka group with four loops (I, II, IV and V), and a chili pepper group with four loops (I, III, IV and V) at the terminal right domain, compared to the CLVd Chaipayon-1 which consists of five loops (I, II, III, IV and V).
Revisiting TNF Receptor-Associated Periodic Syndrome (TRAPS): Current Perspectives
Authors: Cudrici Cornelia , Deuitch Natalie , Aksentijevich Ivona
Published: May 2020

Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) is an autosomal dominant autoinflammatory syndrome characterized by prolonged and recurrent episodes of fever, abdominal and/or chest pain, arthralgia, myalgia, and erythematous rash. TRAPS is associated with heterozygous variants in the TNFRSF1A gene, which encodes the TNFR1 (tumor necrosis factor receptor 1) receptor. Disease-causing variants are found exclusively in the extracellular domain of TNFR1 and affect receptor structure and binding to the TNF ligand. The precise mechanism of the disease is still unclear, but it is thought that intracellular accumulation of misfolded mutant protein leads to endoplasmic reticulum stress and enhanced inflammatory responses through constitutive activation of various immune pathways. Other possible mechanisms contributing to the disease pathogenesis include defective receptor shedding, TNF-induced cell death, production of reactive oxygen species, and autophagy impairment. Patients’ leucocytes are hyperresponsive to stimulation and produce elevated levels of proinflammatory cytokines. Systemic autoimmune (AA) amyloidosis is an important cause of morbidity and mortality in TRAPS. Over the last two decades, new therapies have changed the progression and outcome of the disease. In this review, we summarize clinical data from 209 patients with validated pathogenic variants reported in the literature and discuss TRAPS diagnosis, pathogenesis, and treatment options.