bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatitis E Virus Infection in Patients with Chronic Inflammatory Bowel Disease Treated with Immunosuppressive Therapy
Authors: Kounis Ilias , Renou Christophe , Nahon Stephane , Heluwaert Frederic , Macaigne Gilles , Amil Morgane , Talom Stephane , Lambare Benedicte , Charpignon Claire , Paupard Thierry , Stetiu Monica , Ripault Marie Pierre , Yamaga Armand , Ehrhard Florent , Audemar Franck , Ortiz Correro Maria Carmen , Zanditenas David , Skinazi Florence , Agostini Helene , Coilly Audrey , Roque-Afonso Anne Marie
Published: February 2023

Background: Medical treatment of inflammatory bowel disease (IBD) has evolved significantly, and treatment with immunomodulators is recommended. These medications may alter the patient’s immune response and increase the risk of opportunistic infections. Our aim was to evaluate the prevalence and the incidence of acute or chronic HEV infection in IBD patients under immunomodulatory treatment. Patients and Methods: We conducted a retrospective, multicenter, observational study between 2017 and 2018. IBD outpatients hospitalized for the infusion of immunomodulators were included in 16 French centers. During their daily hospitalization, blood samples were drawn for HEV serology (IgM and IgG) and HEV RNA detection. Results: A total of 488 patients were included, of which 327 (67%) patients had Crohn’s disease and 161 (33%) ulcerative colitis. HEV IgM was detected in 3 patients, but HEV RNA was undetectable in all patients. The HEV IgG seroprevalence rate was 14.2%. IgG-positive patients were older at sampling (p = 0.01) and IBD diagnosis (p = 0.03), had higher seafood consumption (p = 0.01) and higher doses of azathioprine (p = 0.03). Ileal and upper digestive tract involvement was more frequent in IgG-positive patients (p = 0.009), and ileocolic involvement was more frequent in IgG-negative patients (p = 0.01). Under multivariate analysis, age > 50 years [OR: 2.21 (1.26, to 3.85), p = 0.004] was associated with previous HEV infection. Conclusion: Systematic screening for HEV infection is not needed among IBD patients on immunomodulatory medications. However, in the event of abnormal liver test findings, HEV should be part of the classic diagnostic assessment.
Plant-Derived Products with Therapeutic Potential against Gastrointestinal Bacteria
Authors: Qassadi Fatimah I. , Zhu Zheying , Monaghan Tanya M.
Published: February 2023

The rising burden of antimicrobial resistance and increasing infectious disease outbreaks, including the recent COVID-19 pandemic, has led to a growing demand for the development of natural products as a valuable source of leading medicinal compounds. There is a wide variety of active constituents found in plants, making them an excellent source of antimicrobial agents with therapeutic potential as alternatives or potentiators of antibiotics. The structural diversity of phytochemicals enables them to act through a variety of mechanisms, targeting multiple biochemical pathways, in contrast to traditional antimicrobials. Moreover, the bioactivity of the herbal extracts can be explained by various metabolites working in synergism, where hundreds to thousands of metabolites make up the extract. Although a vast amount of literature is available regarding the use of these herbal extracts against bacterial and viral infections, critical assessments of their quality are lacking. This review aims to explore the efficacy and antimicrobial effects of herbal extracts against clinically relevant gastrointestinal infections including pathogenic Escherichia coli, toxigenic Clostridioides difficile, Campylobacter and Salmonella species. The review will discuss research gaps and propose future approaches to the translational development of plant-derived products for drug discovery purposes for the treatment and prevention of gastrointestinal infectious diseases.
Polymeric Nanoparticles as Tunable Nanocarriers for Targeted Delivery of Drugs to Skin Tissues for Treatment of Topical Skin Diseases
Authors: Madawi Eiman Abdalla , Al Jayoush Alaa Raad , Rawas-Qalaji Mutasem , Thu Hnin Ei , Khan Shahzeb , Sohail Mohammad , Mahmood Asif , Hussain Zahid
Published: February 2023

The topical route is the most appropriate route for the targeted delivery of drugs to skin tissues for the treatment of local skin diseases; however, the stratum corneum (SC), the foremost layer of the skin, acts as a major barrier. Numerous passive and active drug delivery techniques have been exploited to overcome this barrier; however, these modalities are associated with several detrimental effects which restrict their clinical applicability. Alternatively, nanotechnology-aided interventions have been extensively investigated for the topical administration of a wide range of therapeutics. In this review, we have mainly focused on the biopharmaceutical significance of polymeric nanoparticles (PNPs) (made from natural polymers) for the treatment of various topical skin diseases such as psoriasis, atopic dermatitis (AD), skin infection, skin cancer, acute-to-chronic wounds, and acne. The encapsulation of drug(s) into the inner core or adsorption onto the shell of PNPs has shown a marked improvement in their physicochemical properties, avoiding premature degradation and controlling the release kinetics, permeation through the SC, and retention in the skin layers. Furthermore, functionalization techniques such as PEGylation, conjugation with targeting ligand, and pH/thermo-responsiveness have shown further success in optimizing the therapeutic efficacy of PNPs for the treatment of skin diseases. Despite enormous progress in the development of PNPs, their clinical translation is still lacking, which could be a potential future perspective for researchers working in this field.
Increasing Bioavailability of Trans-Ferulic Acid by Encapsulation in Functionalized Mesoporous Silica
Authors: Petrișor Gabriela , Motelica Ludmila , Ficai Denisa , Ilie Cornelia-Ioana , Trușcǎ Roxana Doina , Surdu Vasile-Adrian , Oprea Ovidiu-Cristian , Mȋrț Andreea-Luiza , Vasilievici Gabriel , Semenescu Augustin , Ficai Anton , Dițu Lia-Mara
Published: February 2023

Two types of mesoporous materials, MCM-41 and MCM-48, were functionalized by the soft-template method using (3-aminopropyl)triethoxysilane (APTES) as a modifying agent. The obtained mesoporous silica materials were loaded with trans-ferulic acid (FA). In order to establish the morphology and structure of mesoporous materials, a series of specific techniques were used such as: X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Brunauer-Emmet-Teller (BET), Fourier Transform Infrared Spectroscopy (FTIR) and thermogravimetric analysis (TGA). We monitored the in vitro release of the loaded FA at two different pH values, by using simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Additionally, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231 were used to evaluate the antimicrobial activity of FA loaded mesoporous silica materials. In conclusion such functionalized mesoporous materials can be employed as controlled release systems for polyphenols extracted from natural sources.
High Extinction Ratio 4 × 2 Encoder Based on Electro-Optical Graphene Plasma Structure
Authors: Zhu Aijun , Bu Pengcheng , Hu Cong , Niu Junhao , Mahapatra Rabi
Published: February 2023

In this paper, a plasmonic electro-optical encoder based on graphene at THz frequency is proposed. The surface plasmon polaritons (SPPs) in the graphene–insulator–metal structure are excited by an incident TM wave with a wavelength of 9.3 μm. Graphene plasma waveguides have extremely high confinement, relatively low losses, and high tunability. The switching mechanism is based on the application of an external voltage to locally change the chemical potential of the graphene for encoding. Setting the chemical potential to 1 eV allows SPPs to propagate while lowering the chemical potential to 0.1 eV prevents the SPPs from propagating. A 4 × 2 encoder with a minimum encoding extinction ratio (ER) of 37 dB, a maximum modulation depth (MD) of 99.99%, and a structure area of 0.8 μm2 is proposed based on the design rules and simulations using the finite-difference time-domain (FDTD) method. In terms of the obtained results, the proposed structure can be used in optical integrated circuits.
Vineyard Management and Physicochemical Parameters of Soil Affect Native Trichoderma Populations, Sources of Biocontrol Agents against Phaeoacremonium minimum
Authors: Carro-Huerga Guzmán , Mayo-Prieto Sara , Rodríguez-González Álvaro , Cardoza Rosa E. , Gutiérrez Santiago , Casquero Pedro A.
Published: February 2023

Native strains of Trichoderma in vineyard soil represent an opportunity for reducing the incidence of grapevine trunk diseases (GTDs) in vineyards. Moreover, its relationship with the environment (physicochemical soil characteristics and farming management practices) remains unclear. In the current study, a survey was carried out on farming management used by viticulturists, and soil samples were studied to analyze their physicochemical properties and to isolate Trichoderma strains. Later, statistical analyses were performed to identify possible correlations between Trichoderma populations, soil management and soil characteristics. In addition, in vitro tests, including antibiosis and mycoparasitism, were performed to select those Trichoderma strains able to antagonize Phaeoacremonium minimum. In this study a positive correlation was found between the iron content and pH in the soil, and a lower pH increases Trichoderma populations in soils. Vineyard management also affects Trichoderma populations in the soil, negatively in the case of fertilization and tillage and positively in the case of herbicide spraying. Two Trichoderma native strains were selected as potential biocontrol agents (Trichoderma gamsii T065 and Trichoderma harzianum T087) using antibiosis and mycoparasitism as mechanisms of action. These results led to the conclusion that native Trichoderma strains hold great potential as biological control agents and as producers of secondary metabolites.
Identification, Fine Mapping and Application of Quantitative Trait Loci for Grain Shape Using Single-Segment Substitution Lines in Rice (Oryza sativa L.)
Authors: Wang Xiaoling , Li Xia , Luo Xin , Tang Shusheng , Wu Ting , Wang Zhiquan , Peng Zhiqin , Xia Qiyu , Yu Chuanyuan , Xiao Yulong
Published: February 2023

Quantitative trait loci (QTLs) and HQTL (heterosis QTLs) for grain shape are two major genetic factors of grain yield and quality in rice (Oryza sativa L.). Although many QTLs for grain shape have been reported, only a few are applied in production. In this study, 54 QTLs for grain shape were detected on 10 chromosomes using 33 SSSLs (single-segment substitution lines) and methods of statistical genetics. Among these, 23 exhibited significant positive additive genetic effects, including some novel QTLs, among which qTGW4-(1,2), qTGW10-2, and qTGW10-3 were three QTLs newly found in this study and should be paid more attention. Moreover, 26 HQTLs for grain shape were probed. Eighteen of these exhibited significant positive dominant genetic effects. Thirty-three QTLs for grain shape were further mapped using linkage analysis. Most of the QTLs for grain shape produced pleiotropic effects, which simultaneously controlled multiple appearance traits of grain shape. Linkage mapping of the F2 population derived from sub-single-segment substitution lines further narrowed the interval harbouring qTGW10-3 to 75.124 kb between PSM169 and RM25753. The candidate gene was identified and could be applied to breeding applications by molecular marker-assisted selection. These identified QTLs for grain shape will offer additional insights for improving grain yield and quality in rice breeding.
Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (Prunus salicina Lindl.)
Authors: Li Xueling , Cheng Yudou , Wang Ying , Yang Xiaohe , Wei Chuangqi , Guan Junfeng
Published: February 2023

Ethylene is positively correlated with the anthocyanin accumulation in postharvest plum fruit, but the regulation mechanism has not been fully clarified. In this work, the ‘Friar’ plum fruit under different storage temperatures (0, 10 and 25 °C) and treatments (100.0 μL L−1 ethylene and 1.0 μL L−1 1-MCP) were applied to study the relationship between anthocyanin accumulation and ethylene signal pathway. The fruits stored at 10 °C had higher ethylene production rate and more anthocyanin in flesh than those stored at 0 °C and 25 °C. Ten ethylene biosynthesis associated genes and forty-one ethylene signal transduction related genes were obtained from the previous transcriptome data. Among them, the expression levels of ethylene biosynthesis associated genes (PsACS1, PsACS4 and PsACO1), and ethylene signal transduction related genes (PsERS1s, PsETR2, PsERF1a, and PsERF12) were markedly higher in the fruits stored at 10 °C than those at 0 °C and 25 °C. Exogenous ethylene treatment enhanced while 1-MCP treatment inhibited the anthocyanin accumulation in the flesh under storage at 10 °C. In addition, exogenous ethylene treatment markedly increased the expression levels of PsACS1, PsACS4, PsACO1, PsETR2, PsERF1a, and PsERF12 in the flesh once it turning red, as well as the anthocyanin biosynthesis related genes (PsPAL, PsCHS, PsF3H, PsDRF, PsANS, PsUFGT and PsMYB10), whereas 1-MCP treatment manifested the contrary effects. Correlation analysis indicated that there was a significant positive correlation between genes expression related to ethylene signal pathway and anthocyanin biosynthesis, except for PsERF11. In conclusion, ethylene signal pathway is involved in the flesh reddening by up-regulating the anthocyanin biosynthesis related genes.
Applicability of Different Methods for Quantifying Virucidal Efficacy Using MENNO Florades and Tomato Brown Rugose Fruit Virus as an Example
Authors: Nourinejhad Zarghani Shaheen , Ehlers Jens , Monavari Mehran , von Bargen Susanne , Hamacher Joachim , Büttner Carmen , Bandte Martina
Published: February 2023

After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) shall be disinfected. Therefore, the selection of an effective disinfectant plays an important role. With the availability of different methods for virus quantification, we investigated the application of quantitative ELISA (qELISA), RT-qPCR (reverse transcription-quantitative polymerase chain reaction), and bioassays for the quantification of disinfectant efficacy. Therefore, we estimated the titer reduction in tomato brown rugose fruit virus (ToBRFV), a regulated pathogen, in plant sap and on germ carriers after treatment with MENNO Florades 4% for 16 h. The virus load before and after the treatment was measured with the mentioned methods. The RT-qPCR and qELISA methods showed very low efficacy in the presence of the disinfectant. Although bioassays are time-consuming, need purified particles for establishing the quantification models, and are less sensitive than RT-qPCR, they were able to quantify the differences in virus titer in the presence/absence of disinfectant. Interestingly, the bioassays reached at least the lower limit sensitivity of a qELISA. By being less sensitive to the presence of the disinfectant, bioassays proved to be the only technique for the determination of the disinfectant efficacy against ToBRFV on different germ carriers as well as on virus-infected plant sap.
Flexible Cold Atmospheric Plasma Jet Sources
Authors: Corbella Carles , Portal Sabine , Keidar Michael
Published: February 2023

The properties of non-thermal atmospheric pressure plasma jets (APPJs) make them suitable for industrial and biomedical applications. They show many advantages when it comes to local and precise surface treatments, and there is interest in upgrading their performance for irradiation on large areas and uneven surfaces. The generation of charged species (electrons and ions) and reactive species (radicals), together with emitted UV photons, enables a rich plasma chemistry that should be uniform on arbitrary sample profiles. Lateral gradients in plasma parameters from multi-jets should, therefore, be minimized and addressed by means of plasma monitoring techniques, such as electrical diagnostics and optical emission spectroscopy analysis (OES). This article briefly reviews the main strategies adopted to build morphing APPJ arrays and ultra-flexible and long tubes to project cold plasma jets. Basic aspects, such as inter-jet interactions and nozzle shape, have also been discussed, as well as potential applications in the fields of polymer processing and plasma medicine.
Design, Synthesis, and Characterization of Novel Bis-Uracil Chitosan Hydrogels Modified with Zinc Oxide Nanoparticles for Boosting Their Antimicrobial Activity
Authors: Alharbi Rana A. , Alminderej Fahad M. , Al-Harby Nouf F. , Elmehbad Noura Y. , Mohamed Nadia A.
Published: February 2023

A new series of hydrogels was successfully prepared by incorporating various substituted bisuracil (R-BU) linkages between chitosan Schiff’s base chains (R-BU-CsSB) and between chitosan chains (R-BU-Cs). After protection of the amino groups of chitosan by benzaldehyde, yielding chitosan Schiff’s base (CsSB), the reaction with epichlorohydrin was confined on the -OH on C6 to produce epoxy chitosan Schiff’s base (ECsSB), which was reacted with R-BU to form R-BU-CsSB hydrogels, and finally, the bioactive amino groups of chitosan were restored to obtain R-BU-Cs hydrogels. Further, some R-BU-Cs-based ZnO nanoparticle (R-BU-Cs/ZnONPs) composites were also prepared. Appropriate techniques such as elemental analysis, FTIR, XRD, SEM, and EDX were used to verify their structures. Their inhibition potency against all the tested microbes were arranged as: ZnONPs bio-composites > R-BU-Cs hydrogels > R-BU-CsSB hydrogels > Cs. Their inhibition performance against Gram-positive bacteria was better than Gram-negative ones. Their minimum inhibitory concentration (MIC) values decreased as a function of the negative resonance effect of the substituents in the aryl ring of R-BU linkages in the hydrogels. Compared with Vancomycin, the ZnONPs bio-composites showed superior inhibitory effects against most of the tested Gram-negative bacteria, all inspected Gram-positive ones, and all investigated fungi.
Review on Hybrid Reinforced Polymer Matrix Composites with Nanocellulose, Nanomaterials, and Other Fibers
Authors: Seydibeyoğlu Mehmet Özgür , Dogru Alperen , Wang Jinwu , Rencheck Mitch , Han Yousoo , Wang Lu , Seydibeyoğlu Elif Alyamaç , Zhao Xianhui , Ong Kimberly , Shatkin Jo Anne , Shams Es-haghi Siamak , Bhandari Sunil , Ozcan Soydan , Gardner Douglas J.
Published: February 2023

The use of composite materials has seen many new innovations for a large variety of applications. The area of reinforcement in composites is also rapidly evolving with many new discoveries, including the use of hybrid fibers, sustainable materials, and nanocellulose. In this review, studies on hybrid fiber reinforcement, the use of nanocellulose, the use of nanocellulose in hybrid forms, the use of nanocellulose with other nanomaterials, the applications of these materials, and finally, the challenges and opportunities (including safety issues) of their use are thoroughly discussed. This review will point out new prospects for the composite materials world, enabling the use of nano- and micron-sized materials together and creating value-added products at the industrial scale. Furthermore, the use of hybrid structures consisting of two different nano-materials creates many novel solutions for applications in electronics and sensors.
NiO-MgO Prepared by the Complex-Decomposition Method as a Catalyst for Carbon Dioxide Reforming of Methane
Authors: Wang Ying , Li Bin , Xiao Yong-Shan , Liu Zhong-Wen
Published: February 2023

The NiO-MgO solid solution has been proven to be an efficient catalyst for the carbon dioxide reforming of methane (CRM). However, the challenge is still there for the facilely controlled synthesis of the single-phase solid solution with the uniform composition, and the interactions between NiO and MgO are not consistently correlated with the CRM performance. To address these issues, in this work, the complex-decomposition method was applied to regulate the chemical and structural properties of NiO-MgO catalysts via simply changing the complexing agent, calcination temperature, and Ni/Mg molar ratio. The catalysts were comparatively evaluated for CRM under severe reaction conditions of 750 °C, 0.1 MPa, CH4/CO2 = 1, and a gas hourly space velocity of 60000 mL·g−1·h−1. Irrespective of the complexing agents investigated, NiO-MgO solid solution was exclusively formed. However, the structural and reductive properties of the NiO-MgO catalysts were strongly dependent on the complexing agent, which is reasonably explained as the varied coordinative capabilities of the complexing agent with the metal cations. Moreover, the highest CRM performance, i.e., the initial CH4 conversion of ~86% kept constant for a time-on-stream of 20 h, was achieved over the Ni0.1Mg0.9O catalyst by using glycine as the complexing agent and calcined at 800 °C. The characterization and CRM results vigorously confirmed that a good balance between the sintering and the in situ release of active metallic Ni under CRM reaction conditions was constructed over the NiO-MgO catalyst prepared using glycine as the complexing agent, leading to its highest stability. Considering the simple procedure of the complex-decomposition method and the convenient adjustment of the NiO and MgO interactions by simply changing the complexing agent and calcination temperature, the thus developed catalyst can be applied for extensive understanding the CRM mechanism, and extended for large-scale preparation.
The World Was Their Parish: Evangelistic Work of the Single Female Missionaries from the Methodist Episcopal Church, South, to Korea, 1887–1940
Authors: Santiago-Vendrell Angel , Im Misoon (Esther)
Published: February 2023

The Woman’s Foreign Missionary Society (WFMS) (1897–1909) and the Woman’s Missionary Council (WMC) (1910–1940) of the Methodist Episcopal Church, South (MECS) worked in Korea from 1897 to 1940. Their work used a distinctive mission philosophy, hermeneutics, and implementation of strategies in their encounters with Korean women. Over the course of their years in Korea, Southern Methodist missionary women initiated the Great Korea Revival, established the first social evangelistic centers, educated the first indigenous female church historian, and ordained women for the first time in Korea. This article argues that, even though the missionary activities of the single female missionaries occurred in the context of “Christian civilization” as a mission theory, their holistic Wesleyan missiology departed from the colonial theory of mission as civilization. The first section of the article offers background information regarding the single female missionaries to help understand them. What motivated these females to venture in foreign lands with the Gospel? What was their preparation? The second section presents the religious, cultural, social, and political background of Korea during the time the missionaries arrived. The third section describes and analyzes the evangelistic and social ministries of the female missionaries in the nascent Korean mission. The final section describes and analyzes the appropriation and reinterpretation of the Bible and Christianity by Korean women, especially the work of Korean Bible women and Methodist female Christians in the quest for independence from Japanese control in the Independence Movement of 1919.
A New Portrait of a Daoist Sage: Jean-François Foucquet’s Interpretation of the Dao
Authors: Wei Sophie Ling-chia
Published: February 2023

In the translation history of late imperial China, the Jesuit enterprise played a significant role in translating Western scientific knowledge, a role they performed in tandem with proselytization. The Jesuit Figurists’ re-interpreting and re-writing of the ancient Chinese classics pivoted on symbols, figures, and Chinese characters. The father at the helm of this journey, Joachim Bouvet (1656–1730), embarked on his own Figurist path, navigating by the symbols, figures, and Chinese characters from the Yijing. His followers Joseph Henri Marie de Prémare (1666–1736) and Jean François Foucquet (1665–1741) continued on this track, each further developing his own interpretation of the Dao. Here I will present and explore Foucquet’s journey of the Dao and his presentation of the Christian God and Jesus Christ as Daoist sages by investigating his Chinese, French, and Latin manuscripts that discuss his reinterpretation of the Dao in the Chinese classics, especially the Yijing and Daodejing. In these manuscripts, Foucquet adopted typological exegesis and exhibited his inheritance of the Confucian-Christian-Dao synthesis from his senior Bouvet; he also identified the Dao as Deus and the Oneness of the Dao as the unity of the Holy Trinity. This micro-historical case study of Foucquet’s interpretation of the Dao shows how his navigating the strait between the Scylla and Charybdis of the emperor and the Holy See factored into his trajectory of interpreting the Dao; it also demonstrates that in response to being challenged by his own brothers in the Catholic Church, he cleaved to typological exegesis and Confucian-Christian-Dao synthesis. The significance of this paper lies in that the early understanding of the Dao was manipulated, especially among the Figurists, both as a tool for proselytization and as a bridge to link the East with the West.
Digital Animism: Towards a New Materialism
Authors: Krebs Victor J.
Published: February 2023

With the advent of ‘the virtual world,’ we have naturally gauged the ‘reality’ of the virtual in terms of how close it comes to empirical experience. However, the common association of the virtual to simulation depends on a representational dualism that reduces it to a simulacrum of reality and prevents us from seeing its real import. Virtuality, rather than related to simulation, refers instead to potentiality. Far from being something that first appears with the digital-virtual as a technological simulation, the virtual constitutes the bare potentiality intrinsic to human experience, always subject to technological modulation. Despite the path of increasing abstraction marked by the evolution of the technologies of communication, I argue that the virtual world, paradoxically, reveals matter as ineluctably vital and in permanent movement and transformation. The digital thus does away with the dualism responsible for the modern disenchantment of nature and—decentering the human, placing it as equally part of a rhizomatic and entangled nature—lays the groundwork for an animistic ontology that is consonant with a new materialism.
The Human Early Maternal–Embryonic Interactome
Authors: Stevens Adam , Khashkhusha Taqua , Sharps Megan , Garner Terence , Ruane Peter T. , Aplin John D.
Published: February 2023

Background: Single cell transcriptomics offers an avenue for predicting, with improved accuracy, the gene networks that are involved in the establishment of the first direct cell–cell interactions between the blastocyst and the maternal luminal epithelium. We hypothesised that in silico modelling of the maternal–embryonic interface may provide a causal model of these interactions, leading to the identification of genes associated with a successful initiation of implantation. Methods: Bulk and single cell RNA-sequencing of endometrial epithelium and scRNAseq of day 6 and 7 trophectoderm (TE) were used to model the initial encounter between the blastocyst and the maternal uterine lining epithelium in silico. In silico modelling of the maternal–embryonic interface was performed using hypernetwork (HN) analysis of genes mediating endometrial–TE interactions and the wider endometrial epithelial transcriptome. A hypernetwork analysis identifies genes that co-ordinate the expression of many other genes to derive a higher order interaction likely to be causally linked to the function. Potential interactions of TE with non-ciliated luminal cells, ciliated cells, and glandular cells were examined. Results: Prominent epithelial activities include secretion, endocytosis, ion transport, adhesion, and immune modulation. Three highly correlated clusters of 25, 22 and 26 TE-interacting epithelial surface genes were identified, each with distinct properties. Genes in both ciliated and non-ciliated luminal epithelial cells and glandular cells exhibit significant functional associations. Ciliated cells are predicted to bind to TE via galectin–glycan interaction. Day 6 and day 7 embryonic–epithelial interactomes are largely similar. The removal of aneuploid TE-derived mRNA invoked only subtle differences. No direct interaction with the maternal gland epithelial cell surface is predicted. These functional differences validate the in silico segregation of phenotypes. Single cell analysis of the epithelium revealed significant change with the cycle phase, but differences in the cell phenotype between individual donors were also present. Conclusions: A hypernetwork analysis can identify epithelial gene clusters that show correlated change during the menstrual cycle and can be interfaced with TE genes to predict pathways and processes occurring during the initiation of embryo–epithelial interaction in the mid-secretory phase. The data are on a scale that is realistic for functional dissection using current ex vivo human implantation models. A focus on luminal epithelial cells may allow a resolution to the current bottleneck of endometrial receptivity testing based on tissue lysates, which is confounded by noise from multiple diverse cell populations.
An Iterative Algorithm for Predicting Seafloor Topography from Gravity Anomalies
Authors: Yu Jinhai , An Bang , Xu Huan , Sun Zhongmiao , Tian Yuwei , Wang Qiuyu
Published: February 2023

As high-resolution global coverage cannot easily be achieved by direct bathymetry, the use of gravity data is an alternative method to predict seafloor topography. Currently, the commonly used algorithms for predicting seafloor topography are mainly based on the approximate linear relationship between topography and gravity anomaly. In actual application, it is also necessary to process the corresponding data according to some empirical methods, which can cause uncertainty in predicting topography. In this paper, we established analytical observation equations between the gravity anomaly and topography, and obtained the corresponding iterative solving method based on the least square method after linearizing the equations. Furthermore, the regularization method and piecewise bilinear interpolation function are introduced into the observation equations to effectively suppress the high-frequency effect of the boundary sea region and the low-frequency effect of the far sea region. Finally, the seafloor topography beneath a sea region (117.25°–118.25°E, 13.85°–14.85°N) in the South China Sea is predicted as an actual application, where gravity anomaly data of the study area with a resolution of 1′ × 1′ are from the DTU17 model. Comparing the prediction results with the data of ship soundings from the National Geophysical Data Center (NGDC), the root-mean-square (RMS) error and relative error can be up to 127.4 m and approximately 3.4%, respectively.
A Framework for High-Resolution Mapping of Soil Organic Matter (SOM) by the Integration of Fourier Mid-Infrared Attenuation Total Reflectance Spectroscopy (FTIR-ATR), Sentinel-2 Images, and DEM Derivatives
Authors: Xu Xuebin , Du Changwen , Ma Fei , Qiu Zhengchao , Zhou Jianmin
Published: February 2023

Soil organic matter (SOM), as the greatest carbon storage in the terrestrial environment, is inextricably related to the global carbon cycle and global climate change. Accurate estimation and mapping of SOM content are crucial for guiding agricultural output and management, as well as controlling the climate issue. Traditional chemical analysis is unable to satisfy the dynamic estimation of SOM due to its low timeliness. Remote and proximal sensing have significant advantages in terms of ease of use, estimation accuracy, and geographical resolution. In this study, we developed a framework based on machine learning to estimate SOM with high accuracy and resolution using Fourier mid-infrared attenuation total reflectance spectroscopy (FTIR-ATR), Sentinel-2 images, and DEM derivatives. This framework’s performance was evaluated on a regional scale using 245 soil samples from northeast China. Results indicated that the calibration size could be shrunk to 50% while achieving a fair prediction performance for SOM content. The Lasso, partial least squares (PLS), support vector regression (SVR), and convolutional neural networks (CNN) performed well in predicting SOM from FTIR-ATR spectra, and the performance was enhanced further by using Sentinel-2 images and DEM derivates. The PLS, SVR, and CNN models created SOM maps with higher spatial resolution and variation than the Kriging approach. The PLS and SVR models provided enough variety and were more realistic in the local SOM map, making them usable at the field scale, and the suggested framework took a fresh look at high-resolution SOM mapping.
Comparison of Cloud Properties between SGLI Aboard GCOM-C Satellite and MODIS Aboard Terra Satellite
Authors: Khatri Pradeep , Hayasaka Tadahiro
Published: February 2023

This study presents a comprehensive comparison of Level 2.0 cloud properties between a Second-generation Global Imager (SGLI) aboard the GCOM-C satellite and a Moderate Resolution Imaging Spectroradiometer (MODIS) aboard the Terra satellite, to better understand the qualities of cloud properties obtained from SGLI/GCOM-C launched on 23 December 2017. The cloud pixels identified as water phase by both satellite sensors are highly consistent to each other by more than 90%, although the consistency is only ~60% for ice phase cloud pixels. A comparison of cloud properties—cloud optical thickness (COT) and cloud particle effective radius (CER)—between these two satellite sensors reveals that water and ice cloud properties can have different degrees of agreement depending on underlying surface. The relative difference (RD) values of 22% (18%) and 37% (24%) for water cloud COT (CER) comparison over ocean and land surfaces and respective values of 35% (42%) and 35% (62%) for comparisons of ice cloud properties, and also other comparison metrics, suggest better agreements for water cloud properties than for ice cloud properties, and for ocean surface than for land surface. Though cloud properties differences between MODIS and SGLI can arise from inherent features of cloud retrieval algorithms, such as differences in ancillary data, surface reflectance, cloud droplet size distribution function, model for ice particle habit, etc., this study further identifies the important roles of cloud thickness and Sun and satellite positions for differences in cloud properties between SGLI and MODIS: the differences in cloud properties are found to increase for thinner clouds, higher solar zenith angle, and higher differences in viewing zenith and azimuth angles between these satellite sensors, and such differences are more distinct for water cloud properties than for ice cloud properties.
Rapid Compaction Monitoring and Quality Control of Embankment Dam Construction Based on UAV Photogrammetry Technology: A Case Study
Authors: Yin Han , Tan Chun , Zhang Wen , Cao Chen , Xu Xinchuan , Wang Jia , Chen Junqi
Published: February 2023

The compaction quality of embankment dams directly affects the safe operation of power stations. The traditional monitoring method has the shortcomings of limited sample and time consumption. Compaction quality can be reflected by the compression ratio (CR) of the filling material. A novel method based on unmanned aerial vehicle (UAV) photogrammetry technology, which can rapidly acquire the CR of the entire filling area, is proposed in the present paper. Specifically, the CR nephogram is obtained by processing the terra information of the compaction body collected by the UAV. Validation of the CR results is performed by comparing them with the results obtained via leveling measurements. Mean absolute error between CR results and leveling measurements results is less than 1%, and the corresponding settlement value error is millimeter-level, reflecting a fairly good agreement. Furthermore, the reduced-scale experiment shows that the UAV-based CR method is more stable than manual measurements, and the efficiency is increased by more than five times, which meets the requirements of compaction quality monitoring and quality control. The CR nephogram obtained can reflect the compaction quality information rapidly, comprehensively, and accurately, thereby guiding the quality control of embankment dam construction.
A High Speed MPPT Control Utilizing a Hybrid PSO-PID Controller under Partially Shaded Photovoltaic Battery Chargers
Authors: Al-Muthanna Galal , Fang Shuhua , AL-Wesabi Ibrahim , Ameur Khaled , Kotb Hossam , AboRas Kareem M. , Garni Hassan Z. Al , Mas’ud Abdullahi Abubakar
Published: February 2023

Improving photovoltaic systems in terms of temporal responsiveness, lowering steady-state ripples, high efficiency, low complexity, and decreased tracking time under various circumstances is becoming increasingly important. A particle-swarm optimizer (PSO) is frequently used for maximum power-point tracking (MPPT) of photovoltaic (PV) energy systems. However, during partial-shadowing circumstances (PSCs), this technique has three major drawbacks. The first problem is that it slowly converges toward the maximum power point (MPP). The second issue is that the PSO is a time-invariant optimizer; therefore, when there is a time-variable shadow pattern (SP), it adheres to the first global peak instead of following the dynamic global peak (GP). The third problem is the high oscillation around the steady state. Therefore, this article proposes a hybrid PSO-PID algorithm for solving the PSO’s three challenges described above and improving the PV system’s performance under uniform irradiance and PSCs. The PID is designed to work with the PSO algorithm to observe the maximum voltage that is calculated by subtracting from the output voltage of the DC-DC boost converter and sending the variation to a PID controller, which reduces the error percentage obtained by conventional PSO and increases system efficiency by providing the precise converter-duty cycle value. The proposed hybrid PSO-PID approach is compared with a conventional PSO and bat algorithms (BAs) to show its superiority, which has the highest tracking efficiency (99.97%), the lowest power ripples (5.9 W), and the fastest response time (0.002 s). The three aforementioned issues can be successfully solved using the hybrid PSO-PID technique; it also offers good performance with shorter times and faster convergence to the dynamic GP. The results show that the developed PID is useful in enhancing the conventional PSO algorithm and solar-system performance.
Baseflow Variation in Southern Taiwan Basin
Authors: Chen Hsin-Yu , Hsu Yu-Hsiang , Huang Chia-Chi , Yeh Hsin-Fu
Published: February 2023

Baseflow is among the most important components of streamflow. It is the main source of streamflow from groundwater systems in the dry season and also plays an important role as a water resource in the ecological environment and for human activities. In recent years, because of climate change, the number of dry season days in Taiwan has increased, and the wet season has been delayed, resulting in fewer rainy days and increased precipitation intensity. In addition, the spatial distribution of rainfall is uneven, and rivers are short and fast-flowing. Taiwan has become a country with abundant rainfall but insufficient water resources; therefore, the assessment of baseflow is important. This study selected eight basins with distinct wet and dry seasons in southern Taiwan as the study area. The baseflow characteristics and their relationships with climate features were assessed using time series analysis, baseflow attribution analysis, and wavelet analysis. The results showed that baseflow has an increasing trend; both precipitation and evapotranspiration have a significant positive correlation with baseflow, and the impact of precipitation is greater than that of evaporation. Sensitivity analysis showed that baseflow increases with increasing evaporation and precipitation; this behavior is related to the concentration of precipitation and evaporation in the wet season. Baseflow attribution analysis showed that the contribution of climate change to baseflow (75.0%) was larger than that of human activities (−2.9%), indicating that climate change was the main factor in the increase in baseflow. Wavelet analysis showed that both the El Niño–Southern Oscillation (ENSO) and Pacific Decadal Oscillation (PDO) are correlated with baseflow, where the PDO is more strongly correlated than the ENSO. The main timescales of the ENSO and PDO are a 4–8-year band and an 8-year band, respectively. The ENSO may have a timescale above the 8-year band, and the PDO exhibits periodic correlation changes at a 1-year band.
Stability Analysis of Multi-Layer Highwall Mining: A Sustainable Approach for Thick-Seam Open-Pit Mines
Authors: Tian Ya , Tu Lixiao , Lu Xiang , Zhou Wei , Jiskani Izhar Mithal , Liu Fuming , Cai Qingxiang
Published: February 2023

Open-pit mining is a common method for extracting coal, but considerable resources are often left unrecovered at the bottom of end-slopes, leading to a permanent waste of resources. This research presents a sustainable approach of multi-layer highwall mining at different levels to address the issue of abundant resources left unrecovered at the bottom of the end-slope in thick-seam open-pit mines. The interlayer between the upper and lower entries is simplified into a beam structure model, the bending moment distribution characteristics of the beam under a load of highwall miner are analyzed, and a method for calculating the thickness range of the interlayer is proposed. The web pillar width and interlayer thickness, obtained theoretically, are verified through a numerical simulation, and the results of mining a single layer are compared to those of mining multiple layers. The results show that the web pillar width and interlayer thickness derived from the numerical simulation are basically the same as those of the theoretical analysis. Compared with single layer mining, the vertical stress on the web pillar in the lowest panel is reduced by 14.83~18.25%, and the safety factor of the web pillar is increased to 0.27. The web pillars and interlayers at different elevations are stable during multi-layer highwall mining. These findings support the feasibility of multi-layer highwall mining for resource recovery, which is conducive to sustainable mining.
Impact of COVID-19 Pandemic on Fisheries Sector and Actions Taken to Cope with the Situation: A Case Study from a Top Fish-Producing Country
Authors: Shamsuddin Md , Hossain Mohammad Belal , Rahman Moshiur , Tazim Md. Farhan , Ali Md. Romjan , Kawla Mst Salamun , Begum Tajmahal , Albeshr Mohammed Fahad , Arai Takaomi
Published: February 2023

Bangladesh, one of the top fish producers in the world, has rapidly been expanding its annual fish production. However, the COVID-19 pandemic and related preventative measures have had a substantial impact on the nation’s fishing sector. In this study, a survey was performed in the Brahmanbaria subdistrict of Bangladesh to assess the impact of COVID-19 and the subsequent efforts made by the Department of Fisheries (DoF), Bangladesh, to mitigate the negative impact on the culture and capture fisheries. The socioeconomic profile of fishery stakeholders for the years 2019, 2020, and 2021 was used to assess the impact of the COVID-19 pandemic situation on fish farming, fish hatcheries, fish nurseries, the fish feed industry, and the dry fish industry. Data analysis showed that the COVID-19 pandemic had a negative impact on the income of fishery stakeholders and their livelihoods. The income of fish farmers decreased by 47.49% in 2020 as compared to the base year of 2019 but increased by 129.34% in 2021, showing the effects of COVID-19 and mitigation efforts. Transport and movement restrictions adversely affected the culture fisheries while favouring capture fisheries with an increased annual catch. To counter or reduce most of those issues, the DoF, Bangladesh, took necessary steps, such as constructing a virtual control room and engaging the fishermen in some unique activities. Fishermen and other stakeholders benefitted because of farm visits, online training, improved interdepartmental coordination, monitoring of fish-selling marketing channels, activation of the FIAC (Farmer’s Information and Advice Centre), and women’s engagement through the provision of subsidies and loans. The DoF constructed fish sanctuaries and implemented law enforcement in 2020 and 2021 to safeguard the habitat for small indigenous species (SIS). These actions might have improved the stakeholders’ income and the post-pandemic scenario by increasing fish productivity. However, further study is recommended on the effective mitigation measures for drawing a clear conclusion.
Isolation and Identification of New Soil Strains with Phosphate-Solubilizing and Exopolysaccharide-Producing Abilities in the Yellow River Wetland Nature Reserve of Luoyang City, China
Authors: Sun Xiaofei , Niu Yuting , Du Yaoze , Geng Chenxue , Guo Chunli , Zhao Lina
Published: February 2023

The establishment of the Yellow River wetland nature reserves improves the local soil structure and fertility through the long-term succession of microorganisms. However, little is known about which indigenous microbial resources can accelerate the process of soil improvement and ecology restoration. To fill this gap, exopolysaccharides-producing bacteria and phosphate-solubilizing bacteria were isolated from soil samples of the wetland nature reserve with higher soil organic matter, available phosphorus, and available nitrogen content. 16S rRNA nucleotide sequence homology analysis and physiological-biochemical assay showed that the strain PD12 with the highest phosphate solubilization activity and higher EPS production was identified as Klebsiella variicola, and other high yield EPS-producing strains (EPS12, EPS15, EPS18, and EPS19) were identified as Pseudomonas migulae, Pseudomonas frederiksbergensis, Aeromonas media, and Pseudomonas vancouverensis, respectively. These results provided new potential microbial resources for the research and development of biofertilizers and added new insights into accelerating the restoration of physical, chemical, and biological properties of soil in the Yellow River basin.