bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Advances in the Application of High Performance Liquid Chromatography in the Analysis of Polyphenols in Wine and Propolis
Authors: Medić-Šarić Marica , Rastija Vesna , Bojić Mirza
Published: July 2020

Wine and propolis are among the richest sources of polyphenolics. Flavonoids and phenolic acids are main pharmacological components whose benefits have been shown in numerous reports. This paper presents a review of the latest applications of HPLC for determination of polyphenolics in propolis and wine. Special attention is focused on sample preparation, chromatographic conditions, and detection of polyphenolics. HPLC will undoubtedly be the most used method that is readily available for analysis, as well as for isolation of polyphenolics in complex mixtures.
Current Perspectives and Recommendations for the Development of Mass Spectrometry Methods for the Determination of Allergens in Foods
Authors: Johnson Phil E , Baumgartner Sabine , Aldick Thomas , Bessant Conrad , Giosafatto Valeria , Heick Julia , Mamone Gianfranco , O'connor Gavin , Poms Roland , Popping Bert , Reuter Andreas , Ulberth Franz , Watson Andrew , Monaci Linda , Mills E N Clare
Published: July 2020

Allergen detection and quantification is an essential part of allergen management as practiced by food manufacturers. Recently, protein MS methods (in particular, multiple reaction monitoring experiments) have begun to be adopted by the allergen detection community to provide an alternative technique to ELISA and PCR methods. MS analysis of proteins in foods provides additional challenges to the analyst, both in terms of experimental design and methodology: (1) choice of analyte, including multiplexing to simultaneously detect several biologically relevant molecules able to trigger allergic reactions; (2) choice of processing stable peptide markers for different target analytes that should be placed in publicly available databases; (3) markers allowing quantification (e.g., through standard addition or isotopically labeled peptide standards); (4) optimization of protease digestion protocols to ensure reproducible and robust method development; and (5) effective validation of methods and harmonization of results through the use of naturally incurred reference materials spanning several types of food matrix.
Milk production, nitrogen utilization, and methane emissions of dairy cows grazing grass, forb, and legume-based pastures
Authors: Wilson Randi L , Bionaz Massimo , MacAdam Jennifer W , Beauchemin Karen A , Naumann Harley D , Ates Serkan
Published: July 2020

Achieving high animal productivity without degrading the environment is the primary target in pasture-based dairy farming. This study investigated the effects of changing the forage base in spring from grass-clover pastures to forb or legume-based pastures on milk yield, N utilization, and methane emissions of Jersey cows in Western Oregon. Twenty-seven mid-lactation dairy cows were randomly assigned to one of three pasture treatments: grass-clover-based pasture composed of festulolium, tall fescue, orchardgrass, and white clover (Grass); forb-based pasture composed of chicory, plantain, and white clover (Forb); and legume-based pasture composed of red clover, bird’s-foot trefoil, berseem clover, and balansa clover (Legume). Pastures were arranged in a randomized complete block design with three replicates (i.e., blocks) with each replicate grazed by a group of three cows. Production and nutritive quality of the forages, animal performance, milk components, nitrogen partitioning, and methane emissions were measured. Feed quality and dry matter intake (DMI) of cows were greater (P ≤ 0.05) for Legume and Forb vs. Grass, with consequent greater milk and milk solids yields (P < 0.01). Cows grazing Forb also had more (P < 0.01) lactose and linoleic acid in milk compared with cows grazing the other pastures, and less (P = 0.04) somatic cell counts compared with Grass. Cows grazing Forb had substantially less (P < 0.01) N in urine, milk, and blood compared with cows grazing the other pastures, with not only a greater (P < 0.01) efficiency of N utilization for milk synthesis calculated using milk urea nitrogen but also a larger (P < 0.01) fecal N content, indicating a shift of N from urine to feces. Both Forb and Legume had a diuretic effect on cows, as indicated by the lower (P < 0.01) creatinine concentration in urine compared with Grass. Methane emissions tended to be less (P = 0.07) in cows grazed on Forb vs. the other pastures. The results indicate that Forb pasture can support animal performance, milk quality, and health comparable to Legume pasture; however, Forb pasture provides the additional benefit of reduced environmental impact of pasture-based dairy production.
7 Effect of piglet birth weight and drying on post-natal changes in rectal temperature
Authors: Cooper Naomi , Vande Pol Katherine D , Ellis Michael , Xiong Yijie , Gates Richard
Published: July 2020

The objective of this study was to evaluate the effects of piglet birth weight and drying piglets at birth on post-natal rectal temperatures using a CRD with 2 treatments: 1) Drying (not dried vs. dried at birth with a desiccant); 2) Birth weight [4 within-litter birth weight quartiles (Q1: 1.13 ± 0.33 kg, Q2: 1.43 ± 0.28 kg, Q3: 1.62 ± 0.28 kg, Q4: 1.81 ± 0.28 kg)]. Sows (26) and litters (281 piglets) were randomly allotted to drying treatment and were housed in farrowing crates with a heat lamp; room temperature was set at 22.8°C. Piglets were weighed at birth and rectal temperature measured at 0, 15, 30, 45, 60, 90, 120, 180, 240, and 1440 min after birth. Data were analyzed using PROC MIXED of SAS (SAS Inst. Inc., Cary, NC); the model included fixed effects of litter birth weight quartile and drying treatment and interaction, and time (repeated measure), and random effect of sow. Mean piglet birth weight and rectal temperature at birth were 1.49 ± 0.39 kg and 39.2 ± 0.43°C, respectively. There were no drying by birth weight treatment interactions. Temperatures were similar (P > 0.05) for the drying and birth weight treatments at birth and 240 and 1440 min (Table 1). Drying increased (P < 0.05) rectal temperature from 15 to 180 min; the greatest difference was at 45 min (2.4°C). Temperatures were similar (P > 0.05) for Q2, 3, and 4 from 15 to 180 min. Quartile 1 had a lower (P < 0.05) temperature than the 3 heavier quartiles from 15 to 180 min, except at 120 min when temperatures were similar for Q1 and 2. The lightest piglets exhibited the greatest post-natal temperature decline and drying of piglets at birth reduced the post-natal temperature decline in piglets of all weights.
Experimental investigation on the characteristics of transient electromagnetic radiation during the dynamic fracturing progress of gas-bearing coal
Authors: Xu Xiaomeng , Wang Qiang , Liu Hui , Zhao Wenwen , Zhang Yihuai , Wang Chen
Published: July 2020

There are extensive studies on electromagnetic radiation (EMR) effects during coal and rock deformation and fracturing processes, but few systematic studies on the EMR features of gas-bearing coal under impact failure circumstances. In order to investigate whether the EMR is affected by the gas (CO2,CH4,N2) sorption, coal type and impact energy, we performed a series of impact loading tests on both briquette coal specimens (BCSs) and raw coal specimens (RCSs) at various pore pressures (0–1.5 MPa). We developed a drop hammer test apparatus to allow impact loading tests on gas-bearing coal, and recorded the EMR signal of the damage of coal simultaneously. The result showed that (i) the amplitude range of the EMR signal generated by the impact damage of the gas-bearing coal is approximately 10–600 mV, with an effective duration time of 3–1500 ms and accumulated energy of 0.1–1000 μJ; (ii) when pore pressure is increased, the maximum amplitude, duration and pulse counts of the EMR decrease accordingly; (iii) the coal powder size and impact energy affect damage features and EMR characteristics, where a higher impact impulse causes more severe damage to coal specimens and (iv) the influence of the presence of adsorptive gas on the EMR signal caused by the destruction of coal bodies has both enhancement and reduction effects. During the early warning assessment of coal and gas outbursts using EMR parameters, not all the waveform parameters are sensitive to coal mine methane, but the significant effect of methane on signal volatility should be considered.
Estimating Carbon Stock of Live Trees Located on the Main Campus of the University of Georgia
Authors: Fox William , Dwivedi Puneet , Lowe Roger C , Welch Sarah , Fuller Madisen
Published: July 2020

We developed a case study for estimating carbon stock (stored and annually sequestered) in aboveground and belowground portions of all the live trees located on the main campus of the University of Georgia. We recorded species, diameter at breast height, and height of trees located between East Broad Street and Carlton Street (north–south direction) and East Campus Road and Lumpkin Street (east–west direction) covering an area of 94.1 hectares. We used i-Tree Eco V6 for estimating carbon stock. There are 6,915 trees in the study area, out of which 73.0 percent (5,049 trees), 32.3 percent (2,236 trees), and 0.7 percent (50 trees) are native, understory, and invasive, respectively. The total carbon stored in trees is 3,450.4 t (SD = 65), and the annual sequestration rate is about 65 t. The University of Georgia should adopt a multifaceted approach for offsetting or reducing the overall carbon emissions, as annual sequestered carbon in measured trees is only 0.11 percent of the total carbon emitted by the university in 2018. This study highlights the role of trees in meeting the carbon reduction challenges faced by colleges and universities across the United States and beyond, and contextualizes the role of green spaces in general, and trees, in particular toward the ongoing movement of sustainable universities and campuses worldwide.

Study Implications: Across the United States and beyond, universities and colleges are actively exploring ways to reduce their overall environmental footprint for achieving sustainable development goals. Trees located on the campuses of universities and colleges provide various ecosystem services, including carbon storage and annual sequestration. We advise that universities and colleges should explore other options to reduce or offset their annual carbon emissions, as the quantity of carbon annually sequestered in trees located on the main campuses could be small relative to their overall annual carbon emissions.
Minimum variance estimation of galaxy power spectrum in redshift space
Authors: Shiraishi Maresuke , Okumura Teppei , Sugiyama Naonori S , Akitsu Kazuyuki
Published: July 2020

We study an efficient way to enhance the measurability of the galaxy density and/or velocity power spectrum in redshift space. It is based on the angular decomposition with the tripolar spherical harmonic (TripoSH) basis and applicable even to galaxy distributions in wide-angle galaxy surveys. While non-trivial multipole-mode mixings are inevitable in the covariance of the Legendre decomposition coefficient commonly used in the small-angle power spectrum analysis, our analytical computation of the covariance of the TripoSH decomposition coefficient shows that such mixings are absent by virtue of high separability of the TripoSH basis, yielding the minimum variance. Via the simple signal-to-noise ratio assessment, we confirm that the detectability improvement by the TripoSH decomposition approach becomes more significant at higher multipole modes, and the hexadecapole of the density power spectrum has two orders of magnitude improvement. The TripoSH decomposition approach is expected to be applied to not only currently available survey data but also forthcoming wide-angle data, and to bring about something new or much more accurate cosmological information.
Stochomys longicaudatus (Rodentia: Muridae)
Authors: Uebbing Severin
Published: July 2020

Stochomys longicaudatus (Tullberg, 1893) is a monotypic African murid rodent called the target rat because long bristle-like hairs in its fur resemble arrows stuck into a target, giving it a unique appearance. Although occurring in comparatively low densities, it is widely spread throughout the lowland rainforests of equatorial western and central Africa. Inhabiting dense understory vegetation, it is a secretive and perhaps nomadic species and little is known about its behavior. S. longicaudatus is herbivorous, climbs well and often, and builds nests from shredded grass. The International Union for Conservation of Nature and Natural Resources classifies it as a species of “Least Concern.”
MEDU-26. LATENT SOX9-POSITIVE CELLS RESPONSIBLE FOR MYC-DRIVEN MEDULLOBLASTOMA RECURRENCE
Authors: Bolin Sara , Savov Vasil , Borgenvik Anna , Rosen Gabriela , Garancher Alexandra , Rahmanto Aldwin Suryo , Hutter Sonja , Mainwaring Oliver , Olausson Karl Holmberg , Rusert Jessica , Sundstrom Anders , Richardson Stacey , Fotaki Grammatiki , Hill Rebecca , Dubuc Adrian , Kalushkova Antonia , Remke Marc , Cancer Matko , Jernberg-Wiklund Helena , Ramaswamy Vijay , Taylor Michael , Sangfelt Olle , Clifford Steven , Schuller Ulrich , Wechsler-Reya Robert , Weishaupt Holger , Swartling Fredrik
Published: July 2020

Tumor recurrence is the leading cause of death among children with medulloblastoma, the most common type of malignant pediatric brain tumors. The mechanisms behind medulloblastoma recurrence are not fully understood. We previously showed that the transcription factor SOX9 promotes cisplatin treatment resistance in medulloblastoma. Here we show that SOX9 levels correlate with poor prognosis in Group 3 tumors. By studying paired primary-recurrent medulloblastoma samples and patient-derived xenograft (PDX) models we further identified rare SOX9-positive slow-cycling, therapy-resistant tumor cells that accumulate in relapses and in leptomenigeal metastases of Group 3 and Group 4 patients. By using an inducible Tet-OFF transgenic (GTML) mouse model for malignant MYCN-driven Group 3 tumors we identified rare SOX9-positive, quiescent brain tumor cells that are more resistant to cisplatin. Dox treatment normally cures GTML transgenic animals that developed aggressive medulloblastoma by turning MYCN off. However, when crossing the Tet-OFF GTML model with a Tet-ON rtTA-Sox9 model we can redirect MYCN expression to the Sox9 promoter ultimately driving brain tumor recurrence from rare SOX9-positive cells with 100% penetrance. In this novel animal model, recurrent tumors were actively disseminating from the hindbrain to the spinal cord and into the forebrain similar to distant relapses found in patients. By overexpressing SOX9 in human Group 3 tumor cells, MYC was directly inhibited and cell proliferation was decreased. PDX models of Group 3 tumors further showed increased levels of SOX9-positivity and less proliferative cells in metastatic compartments. Expression profiling revealed that recurrences were more inflammatory, metastatic, immune evasive and showed elevated MGMT methyltransferase levels which depleted recurrent cells and sensitized them for chemotherapy when using the MGMT inhibitor lomeguatrib. To summarize, our data clarify important and complex mechanisms by which latent medulloblastoma cells fail to respond to standard therapy and generate relapses.
2654. Myocarditis in Dengue: A Prospective Observational Study
Authors: Soneja Manish , Bhatt Manasvini , Farooqui Faraz A , Vikram Naval K , Biswas Ashutosh , Roy Ambuj , Wig Naveet
Published: July 2020

Background

Cardiac involvement in dengue fever is underdiagnosed due to low index of suspicion and overlapping clinical manifestations of capillary leak associated with dengue. The frequency of subclinical dengue myocarditis and its relative contribution to the hemodynamic instability in severe dengue needs to be explored. We studied the prevalence of myocarditis and clinical outcomes among admitted patients with dengue.

Methods

A prospective observational study was carried out in admitted patients with age between 18 and 65 years having confirmed dengue (NS1/IgM ELISA). Patients with electrolyte abnormalities or on medications affecting heat rhythm/ rate, pre-existing heart disease were excluded. The baseline demographic, clinical and laboratory parameters were collected. A baseline ECG was done and repeated every second day. Trop-I and NT-proBNP were done at baseline and repeated only if elevated at baseline or there were ECG changes. The cardiac enzymes were measured using enzyme-linked ﬂuorescent assay (VIDAS, bioMérieux, France). Patients with elevated enzymes underwent 2-dimensional echocardiography. Diagnosis of myocarditis was as per ESC 2013 criteria. Fluid management was as per WHO guidelines (2009).

Results

A total of 183 patients were recruited with median age of 29 years (IQR 21, 37) and 31% were females. Dengue with warning signs was present in 80 (44%) and severe dengue in 45 (25%) patients. Cardiac enzymes were elevated in 27 (15%) patients (cTnI in 25, NT-proBNP in 22). Among these 27 patients, 11 [6% (2.6–9.4, 95% CI)] had echo evidence and diagnosed as having myocarditis according to ESC 2013 criteria (Figure 1). Clinical features of fluid overload were more common in myocarditis group [8 (73%) vs 4 (2%), P = Overall, 5 (2.7%) patients expired, all of them had myocarditis (5/11 = 45%). These patients had severe dengue, 2 patients developed hospital-acquired pneumonia and 1 had malaria co-infection. Among patients with raised enzymes and normal echo (n = 16), 3 patients developed clinical signs of fluid overload compared with only 1 out of 156 patients without raised enzymes (P < 0.01).

Conclusion

Myocarditis in admitted patients with dengue is not uncommon [6% (2.6–9.4, 95% CI)] and may lead to a complicated disease course.

Disclosures

All authors: No reported disclosures.
2802. Occupational Exposure to the Ugandan Strain of Zika Virus in a Laboratory Worker in the United States: Clinical Presentation, Viral Persistence, and Antibody Response
Authors: Lichtenberger Paola , Ricciardi Mike , Solorzano Dalhila , Raccamarich Patricia , Leda Ana R , Sharkey Mark , Walkins David , Mulligan Mark , Doblecki-Lewis Susanne , Stevenson Mario , Alcaide Maria L
Published: July 2020

Background

A laboratory worker suffered an accidental needle stick resulting in infection with the Ugandan strain (MR766) of Zika virus (ZIKV), a strain that has rarely been studied in humans. We report the clinical presentation and outcomes, molecular and serological diagnostic results, and immunological response. A 34-year-old Brazilian-born female laboratory researcher, presented with malaise, skin rash, myalgia and joint pain 10 days after an accidental needle stick while inoculating a mouse with ZIKV-MR766. On physical examination she had bilateral maculopapular rash on the cheeks, and tender effusions at the metacarpal and proximal interphalangeal joints and ankles. Symptoms and signs resolved within 3 weeks. ZIKV infection was confirmed by Nucleic Acid Amplification Test (Lab Corp®) in urine. Serological testing using the ZIKV IgM ELISA test from Lab Corp®, and a confirmatory plaque reduction neutralization test (PRNT) in accordance with the Centers for Disease Control and Prevention (CDC), results were negative.

Methods

Whole blood, plasma, urine, saliva, and a vaginal swab were collected from day (D) 14 post exposure (PE) to D104 PE. A novel, antibody competition-based ZIKV diagnostic test (highly specific for ZIKV antibodies) was performed in serum, and detection of ZIKV-MR766 genomic RNA was performed in all body fluids longitudinally.

Results

Antibody response revealed broad IgM response to both ZIKV-Paraiba (strain from the 2015 outbreak) and ZIKV-MR766 during the acute phase of the infection, suggesting cross-reactivity. There was no cross-reactivity against dengue or yellow fever viruses. An IgG response was detected against both ZIKV strains and increased until D104 PE. ZIKV RNA was detected in whole blood, saliva, urine, and the vaginal swab at D14 PE. At D20 PE, virus was only detectable in whole blood at a value of less than 37 copies per mL. At D23 PE, there was no detectable virus. (figure).

Conclusion

This case highlights the potential for ZIKV occupational exposure. Findings may be useful for the development of diagnostic tests against ZIKV as we were able to accurately determine time of exposure, presence of virus in body fluids, development of symptoms, and antibody responses after a well-documented infection.

Disclosures

All authors: No reported disclosures.
647. Diagnoses Associated with Temperature ≥104°F in Adults
Authors: Chi Sharon , Simon Gary , Weintrob Amy
Published: July 2020

Background

Temperature ≥104°F (T ≥ 104) is uncommon in adults. The diagnoses and clinical characteristics were reviewed for patients with T ≥ 104.

Methods

Infectious disease physicians reviewed charts of patients with T ≥ 104 seen at the Washington DC Veterans Affairs Medical Center from 2009 to 2018. The following was collected: demographics, past medical history, medications, WBC, maximum temperature, time to defervescence, etiology of T ≥ 104, and death.

Results

Less than 0.01% of all patient encounters were associated with T ≥ 104. Of the 60 most recent patients with T ≥ 104 (from 2014 to 2018), the median age was 63.5 years (range 23–97), 65% were African American, 88% were male. 82% of those with T ≥ 104 were hospitalized; 76% of those had the T ≥ 104 on or within 72 hours of admission. 25% of the 60 patients had underlying cancer, 10% HIV, 30% DM, 13% CKD, and 13% were on steroids/immunosuppressants/biologics. The median peak temperature was 104.3°F (interquartile range 104.0 – 104.7); maximum was 106.8°F. 82% had T ≥ 104 for only 1 day and the median time to defervescence was 2 days. There were 55 diagnoses amongst 48 patients; 12 had no identifiable etiology of T ≥ 104. Of the identifiable diagnoses, there were 45 (81.8%) infections, 4 (7.3%) metastatic malignancies (1 Hodgkin’s lymphoma, 1 small cell carcinoma, 1 squamous cell carcinoma, 1 unknown primary), 2 (3.6%) intracranial bleeds, 2 (3.6%) GI bleeds, 1 (1.8%) mixed collagen vascular disease, and 1 (1.8%) neuroleptic malignant syndrome. The most common infections were 15 cases of pneumonia including 2 Legionella, 8 complicated UTI/pyelonephritis, 3 primary bacteremia, 2 West Nile virus, 2 influenza, and 2 cholangitis with bacteremia. The median WBC of infectious diagnoses (9.8) was significantly higher than noninfectious diagnoses (5.8, P = 0.006, T-test). Of the 60 patients, 20% died within 30 days of T ≥ 104 including 2 patients who died of sepsis. 67% of those who died were receiving hospice care.

Conclusion

T ≥ 104 is rare in adults and is usually associated with bacterial infections such as pneumonia (including Legionella), complicated UTIs/pyelonephritis, and primary bacteremia but may also be seen with viral infections such as West Nile virus and influenza. Mortality is high.

Disclosures

All authors: No reported disclosures.
738. Potent In Vitro activity of Rezafungin (RZF) Against Aspergillus Clinical Isolates Recovered From Lung Transplant Patients Who Have Received ≥3 Months of Triazole Prophylaxis
Authors: Driscoll Eileen , Clancy Cornelius J , Nguyen Minh-Hong
Published: July 2020

Background

Emergence of azole resistance globally among Aspergillus species has major one health implications for humans and agriculture. At our center, isavuconazole (ISA), posaconazole (POS) and voriconazole (VOR) have been used as antifungal prophylaxis for at least 3 months or as pre-emptive therapy in solid-organ transplant (SOT) patients. We previously showed that azole breakthrough (BT) fungi were more likely to be non-fumigatus Aspergillus (non-Af) spp. In addition, azole BT isolates exhibited higher azole MICs than non-BT isolates, with 7% pan-azole resistance. RZF is an investigational echinocandin with long serum half-life, suitable for prolonged dosing intervals. We determined caspofungin (CAS) and RZF minimum effective concentrations (MECs) against Aspergillus isolates from our center.

Methods

Aspergillus recovered from 111 patients between December 2016 and April 2018 were tested. MICs (minimum inhibitory concentrations; azoles) and MECs (echinocandins) were measured. Candida parapsilosis ATCC 22019 and Candida krusei ATCC 6258 were used as QC controls.

Results

71% (79) of isolates were from SOT patients. Aspergillus spp. were A. fumigatus (Af, 73), A. flavus (Afl, 12), A. niger (An, 9), A. terreus (At, 8), A. calidoustus (Ac, 7), and A. lentulus (Al), A. glaucus, A. thermomutatus, and A. thermomutatus (At; 1 each). 7% of Aspergillus isolates exhibited VOR, POS and ISA MICs ≥2, ≥8 and ≥1 µg/mL, respectively. RZF MEC50 and range of MEC by Aspergillus spp.are summarized in the Table. Overall, there was no difference in MECs between CAS and RZF (P = 0.21). 6% (7) and 7% (8) of the non-Af isolates exhibited CAS and RZF MECs >0.5 µg/mL, respectively. 5 isolates exhibited CAS and RZF MEC ≥16 µg/mL.

Conclusion

Despite concerns over azole resistance among Aspergillus, these agents remain frontline against invasive aspergillosis. The excellent activity of RZF and CAS shown here suggests that the drugs are potential therapeutic options for patients infected with azole BT Aspergillus, including azole-resistant isolates. The long-half-life and high tolerability of RZF make this agent an attractive consideration for antifungal prophylaxis. A clinical trial of RZF prophylaxis in stem cell transplant recipients is planned.

Disclosures

All authors: No reported disclosures.
Multistage Hybrid Approach to Management of Significant Sagittal Malalignment: 2-Dimensional Operative Video
Authors: Godzik Jakub , Hartman Cory , Wewel Joshua T , Walker Corey T , Xu David S , Uribe Juan S
Published: July 2020

Minimally invasive surgery (MIS) approaches for the correction of adult spinal deformity have gained popularity in the past decade. MIS approaches can result in decreased hospitalization times and decreased morbidity. However, compared to open techniques, MIS approaches are challenging in the setting of fixed sagittal deformity and strategic surgical staging. Combined MIS and miniopen techniques are described as “hybrid” techniques. We report on the surgical approach for a fixed sagittal deformity using both MIS and miniopen techniques, specifically a miniopen pedicle subtraction osteotomy (PSO) and an anterior column release (ACR). The patient gave written informed consent for surgical treatment; institutional review board approval was not required. The patient first underwent the placement of percutaneous modular pedicle screws from T12 to the pelvis as well as a mini-PSO across the previously fused L5 vertebral body, with the placement of a temporary rod. The following day, the patient underwent lateral transpsoas interbody fusion and ACR at L2/3; a percutaneous rod was then passed from T12 to the pelvis for segmental fixation. The patient recovered well and was discharged home without complication 6 d after the initial day of surgery. The combined use of surgical staging and traditional open techniques in a selective, minimalistic fashion and adherence to minimally invasive principles provide for a powerful set of surgical techniques that capitalize on less invasive approaches to deformity management. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.
Operative Nuances of Blind Catheterization of Occluded Sinuses Using Negative Road Map Technique: 2-Dimensional Operative Video
Authors: Brunozzi Denise , Alaraj Ali
Published: July 2020

Dural arteriovenous fistula (DAVF) are often treated with endovascular transvenous embolization. DAVFs though, are often associated with intracranial venous sinus occlusion, which limits the transvenous route.

 Here, we present the operative nuances of blind catheterization of an occluded dural venous sinus in 2 different cases with DAVF. First case is a 72-yr-old patient with indirect right carotid-cavernous fistula associated with an occluded inferior petrosal sinus, with severe orbital congestion. Second patient is a 79-yr-old patient with a new external carotid to a trapped transverse sinus fistula (Cognard IIA + B), extensive cortical venous reflux in the setting of an occluded sigmoid sinus. In both cases, the transarterial route was limited because of small arterial feeders thus, after obtaining patients’ consent, we performed transvenous sacrifice of the isolated sinus. In both cases, the occluded sinus was transvenously blindly retrograde probed using a 0.035 inch Terumo Glidewire (Terumo Medical Corporation, Somerset, New Jersey). Once the occluded segment was probed, a dark roadmap was acquired with the wire in place. This created a negative roadmap once the guidewire is removed. This negative roadmap is used to navigate the microcatheter-microwire into the isolated sinus. In the first case, the cavernous sinus and the superior ophthalmic veins were sacrificed with coils. In the second patient, the entrapped left transverse sinus was embolized using Onyx. In both cases, complete occlusion of the fistula was attained.

 In this neuroendovascular video, we demonstrate the nuances of blind catheterization of an occluded sinus using a negative roadmap technique as guidance for the micro-catheterization.

 Institutional Review Board approved. Patient consent not required due to retrospective nature of manuscript, based on medical chart and imaging reviews, anonymized in the video.
Subaru Hyper Suprime-Cam view of quasar host galaxies at z < 1
Authors: Ishino Toru , Matsuoka Yoshiki , Koyama Shuhei , Saeda Yuya , Strauss Michael A , Goulding Andy D , Imanishi Masatoshi , Kawaguchi Toshihiro , Minezaki Takeo , Nagao Tohru , Noboriguchi Akatoki , Schramm Malte , Silverman John D , Taniguchi Yoshiaki , Toba Yoshiki
Published: July 2020

Active galactic nuclei (AGNs) are key in understanding the coevolution of galaxies and supermassive black holes (SMBHs). AGN activity is thought to affect the properties of their host galaxies via a process called “AGN feedback,” which drives the coevolution. From a parent sample of 1151 z < 1 type-1 quasars from the Sloan Digital Sky Survey quasar catalog, we detected the host galaxies of 862 of them in the high-quality grizy images of the Subaru Hyper Suprime-Cam survey. The unprecedented combination of the survey area and depth allowed us to perform a statistical analysis of the quasar host galaxies, with small sample variance. We fitted the radial image profile of each quasar as a linear combination of the point spread function and the Sérsic function, decomposing the images into the quasar nucleus and the host galaxy components. We found that the host galaxies are massive, with stellar mass Mstar ≳ 1010 $M_\odot$, and are mainly located on the green valley. This trend is consistent with a scenario in which star formation in the host galaxies is suppressed by AGN feedback, that is, AGN activity may be responsible for the transition of these galaxies from the blue cloud to the red sequence. We also investigated the SMBH mass to stellar mass relation of the z < 1 quasars, and found a consistent slope with the local relation, while the SMBHs may be slightly undermassive. However, the above results are subject to our sample selection, which biases against host galaxies with low masses and/or large quasar-to-host flux ratios.
Application of High-Intensity Functional Resistance Training in a Skilled Nursing Facility: An Implementation Study
Authors: Gustavson Allison M , Malone Daniel J , Boxer Rebecca S , Forster Jeri E , Stevens-Lapsley Jennifer E
Published: July 2020

Objective

Rehabilitation in skilled nursing facilities (SNFs) is under scrutiny to deliver high-quality care and superior outcomes in less time. High-intensity resistance training demonstrates functional improvements in community-dwelling and long-term care populations but has not been generalized to the SNF population. The purpose of this study was to evaluate implementation issues including safety and feasibility and to provide preliminary information on effectiveness of rehabilitation focused on high-intensity functional resistance training in an SNF.

Methods

The implementation study design consisted of 2 nonrandomized independent groups (usual care and high intensity) that were staged within a single SNF. The i-STRONGER program (IntenSive Therapeutic Rehabilitation for Older Skilled NursinG HomE Residents) integrates principles of physiologic tissue overload into rehabilitation. Physical therapists administered the Short Physical Performance Battery and gait speed at evaluation and discharge. Reach, Effectiveness, Adoption, Implementation, and Maintenance was used to evaluate the implementation process. An observational checklist and documentation audits were used to assess treatment fidelity. Regression analyses evaluated the response of functional change by group.

Results

No treatment-specific adverse events were reported. Treatment fidelity was high at >99%, whereas documentation varied from 21% to 50%. Patient satisfaction was greater in i-STRONGER, and patient refusals to participate in therapy sessions trended downward in i-STRONGER. Patients in i-STRONGER exhibited a 0.13 m/s greater change in gait speed than in the usual care group. Although not significant, i-STRONGER resulted in a 0.64-point greater change in the Short Physical Performance Battery than usual care, and average SNF length of stay was 3.5 days shorter for i-STRONGER patients.

Conclusion

The findings from this study indicate that implementation of a high-intensity resistance training framework in SNFs is safe and feasible. Furthermore, results support a signal effectiveness of improving function and satisfaction, although the heterogeneity of the population necessitates a larger implementation study to confirm.

Impact Statement

This pragmatic study demonstrates that high-intensity resistance training in medically complex older adults is safe and favorable in SNFs. This work supports the need to fundamentally change the intensity of rehabilitation provided to this population to promote greater value within post-acute care. Furthermore, this study supports the application of implementation science to rehabilitation for rapid and effective translation of evidence into practice.
Site Index Curves for Direct-Seeded Loblolly and Longleaf Pines in Louisiana
Authors: Cao Quang V. , Baldwin V. Clark , Lohrey Richard E.
Published: July 2020

Site index equations were developed for direct-seeded loblolly pine (Pinus taeda L.) and longleaf pine (Pinus palustris Mill.) based on data from 148 and 75 permanent plots, respectively. These plots varied from 0.053 to 0.119 ac in size, and were established in broadcast, row, and spot seeded stands throughout Louisiana. The Bailey and Clutter (1974) model was selected for stand height prediction. Site index curves are presented for both species based on these equations. These site index models should provide satisfactory short-term height projection for direct-seeded loblolly and longleaf pine stands in Louisiana. South. J. Appl. For. 21(3):134-138.
Contextualizing Educational Disparities and the Evaluation of Teacher Quality
Authors: Shifrer Dara
Published: July 2020

Value added scores, statistical estimates of teacher quality, are representative of neoliberal logic. The higher average scores of teachers of socially advantaged students raise concerns that scores are inaccurate and unfair, and propagate decontextualized neoliberal understandings of the nature of learning and teachers’ work. This study uses longitudinal data from roughly 4,500 teachers in a large urban district between 2007–08 through 2012–13 to follow individual teachers as they switch into schools of different “performance levels” over time. Fixed-intercept models tracking individual teachers between 2007–08 and 2012–13 showed scores increased for teachers who switched into high-performing schools and decreased for teachers who switched into low-performing schools. Particularly indicative of scores biased by contextual factors outside teachers’ control, score changes for mobile teachers are partially attributable to shifts in the economic status and race of students in teachers’ classrooms and schools. Understanding how neoliberalism operates within education provides sociological insight into how neoliberalism is legitimated and perpetuated in other central social institutions, such as the criminal justice system, the environment, gender, sexuality, and health.
Effect of rumen-protected lysine on growth performance, carcass characteristics, and plasma amino acid profile in feedlot steers
Authors: Heiderscheit Katie J , Hansen Stephanie L
Published: July 2020

The objective of this study was to evaluate growth performance, carcass characteristics, and plasma amino acid profiles of feedlot steers fed rumen-protected Lys. Forty-two Angus-cross steers (304 ± 25 kg) were blocked by weight and fed treatment diets for 180 d (growing days 0 to 55; finishing days 56 to 180): 1) Lys-deficient diet (CON; n = 12 steers), 2) Lys-adequate diet containing soybean meal (POS; n = 12 steers), or 3) Lys-deficient diet plus supplemental rumen-protected Lys (RPL; AjiPro-L; Ajinomoto Animal Nutrition North America, Eddyville, IA; n = 18 steers). Consecutive day bodyweights (BWs) were recorded to begin and end growing and finishing. Individual steer dry matter intake (DMI) was recorded. Blood was collected on days 0, 56, and 179 for analysis of physiological free amino acids. Steers were harvested on day 180 and carcass characteristics were recorded. Data were analyzed using Proc Mixed of SAS 9.4. Steer was the experimental unit and treatment was the fixed effect for all parameters. Block was a fixed effect for growth performance, feed intake, and carcass data. The day 0 value for each parameter of physiological free amino acids was used as a covariate during analysis. The CON steers had greater BW, average daily gain (ADG), and gain to feed (G:F) at the end of growing (day 56; P ≤ 0.05) vs. POS and RPL. The CON steers also had greater final BW (P = 0.04) and overall ADG (P = 0.04) than RPL, while POS was intermediate. Carcass characteristics were not different across treatments [hot carcass weight, dressing percent, ribeye area, back fat, kidney/pelvic/heart (KPH) percent, marbling, or calculated yield grade; P ≥ 0.13]. Plasma urea N was greater in POS steers on days 56 and 179 (P ≤ 0.04). Plasma Lys and Arg concentrations were greater in POS at day 56 (P ≤ 0.02); however, there was no difference among treatments for these two variables at day 179 (P ≥ 0.44). Steers in all treatments had greater DMI than predicted, causing a negative metabolizable Lys balance for all treatments during growing. Though the metabolizable Lys balance was positive for POS and RPL-fed steers during finishing, the increased metabolizable Lys in these treatments may have decreased performance if other amino acids were imbalanced due to increased intakes.
Alogliptin: a novel approach against cyclophosphamide-induced hepatic injury via modulating SIRT1/FoxO1 pathway
Authors: Salama Rania M , Mohamed Abdelkader M , Hamed Nada S , Ata Raneem M , NourelDeen Amira S , Hassan Mohamed A
Published: July 2020

Cyclophosphamide (CP) is one of the most potent alkylating agents and is widely used in the treatment of numerous neoplastic conditions, autoimmune diseases and following organ transplantation. Due to its ability to induce oxidative stress and subsequent apoptosis, CP is affiliated with many adverse effects with special emphasis on the highly prevalent hepatotoxicity. Dipeptidyl peptidase 4 (DDP-IV) inhibitors are being rediscovered for new biological effects due to their ability to target multiple pathways, among which is the phosphoinositide 3–kinase (PI3K) and protein kinase B (Akt) axis. This could offer protection to multiple organs against reactive oxygen species (ROS) through modulating sirtuin 1 (SIRT1) expression and, in turn, inactivation of forkhead box transcription factor of the O class 1 (FoxO1), thus inhibiting apoptosis. Accordingly, the current study aimed to investigate the potential therapeutic benefit of alogliptin (Alo), a DPP-IV inhibitor, against CP-induced hepatotoxicity through enhancing PI3K/Akt/SIRT1 pathway. Forty male Wistar rats were randomly divided into four groups. The CP-treated group received a single dose of CP (200 mg/kg; i.p.). The Alo-treated group received Alo (20 mg/kg; p.o.) for 7 days with single CP injection on Day 2. Alo successfully reduced hepatic injury as witnessed through decreased liver function enzymes, increased phospho (p)-PI3K, p-Akt, superoxide dismutase (SOD) levels, SIRT1 expression, p-FoxO1 and anti-apoptotic B-cell lymphoma 2 (Bcl-2). This resulted in decreased apoptosis, as witnessed through decreased caspase-3 levels and improved histopathological picture. In conclusion, the current study succeeded to elaborate, for the first time, the promising impact of Alo in ameliorating chemotherapy-induced liver injury.
Microscopic and biochemical changes on liver and kidney of Wistar rats on combination antiretroviral therapy: the impact of naringenin and quercetin
Authors: Akang Edidiong Nnamso , Dosumu Olufunke O , Okoko Ini-ibehe Essien , Faniyan Oluwatomisin , Oremosu Ademola A , Akanmu Alani Sulaimon
Published: July 2020

Combination antiretroviral therapy (cART), which is a lifelong therapy for people living with human immunodeficiency virus, has been associated with nephrotoxicity and hepatotoxicity leading to its discontinuation. This study aimed at investigating the ameliorative potential of naringenin and quercetin on cART-induced hepatotoxicity and nephrotoxicity. Seventy male Wistar rats (225–260 g) were divided into seven groups as control, cART, naringenin, quercetin, dimethyl sulfoxide (DMSO), naringenin/cART (CN) and quercetin/cART (CQ). cART (24 mg/kg), naringenin (50 mg/kg) and quercetin (50 mg/kg) were dissolved in 1% v/v DMSO and administered orally for 56 days. Combination of cART and bioflavonoids had significant increase in superoxide dismutase (P < 0.05), catalase (P < 0.01), reduced glutathione (P < 0.001) and decreased malondialdehyde (P < 0.001) compared to cART only. Tumor necrosis factor Alpha (TNFα) level increased significantly in cART and CQ (P < 0.01) groups, while others showed no significant changes compared to control. TNFα also significantly decreased in CQ level compared to cART (P < 0.001). In addition, significant increase in creatinine level in cART only indicated progressive renal toxicity. Also, progressive pathological changes including congested blood vessels and hepatocellular necrosis were found in the liver, while the kidney had glomerular atrophy, and tubular distortion in cART-only group. Control, naringenin- and quercetin-treated groups showed normal renal and hepatic cytoarchitecture. These findings elucidate that progressive renal and hepatic toxicity is associated with the continuous use of cART; however, a combination of quercetin and naringenin with cART showed possible potential of ameliorating the damages posed by cART.
PtKTI12genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar
Authors: Wang Hailang , Xu Chao , Zhang Youbing , Yan Xu , Jin Xiaohuan , Yao Xiaoqing , Chen Peng , Zheng Bo
Published: July 2020

The multisubunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the Elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular 5-carbamoylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine. Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. Also, the protein products of PtKTI12A and PtKTI12B had similar subcellular localization and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar.
Developing consensus of evidence to target case finding surveys for podoconiosis: a potentially forgotten disease in India
Authors: Simpson Hope , Panicker K N , George Leyanna Susan , Cano Jorge , Newport Melanie J , Davey Gail , Deribe Kebede
Published: July 2020

Background

Podoconiosis is a non-infectious geochemical lymphoedema of the lower legs associated with a significant burden of morbidity. There are historical reports of podoconiosis in India, but its current endemicity status is uncertain. In this investigation we aimed to prioritise the selection of districts for pilot mapping of podoconiosis in India.

Methods

Through a consultative workshop bringing together expert opinion on podoconiosis with public health and NTDs in India, we developed a framework for the prioritisation of pilot areas. The four criteria for prioritisation were predicted environmental suitability for podoconiosis, higher relative poverty, occurrence of lymphoedema cases detected by the state health authorities and absence of morbidity management and disability prevention (MMDP) services provided by the National Programme for Elimination of Lymphatic Filariasis.

Results

Environmental suitability for podoconiosis in India was predicted to be widespread, particularly in the mountainous east and hilly southwest of the country. Most of the districts with higher levels of poverty were in the central east and central west. Of 286 districts delineated by state representatives, lymphoedema was known to the health system in 189 districts and not recorded in 80. Information on MMDP services was unavailable for many districts, but 169 were known not to provide such services. We identified 35 districts across the country as high priority for mapping based on these criteria.

Conclusions

Our results indicate widespread presence of conditions associated with podoconiosis in India, including areas with known lymphoedema cases and without MMDP services. This work is intended to support a rational approach to surveying for an unrecognised, geographically focal, chronic disease in India, with a view to scaling up to inform a national strategy if required.
Pattern of Deterioration and Recovery of Wood from Dead Yellow-Cedar in Southeast Alaska
Authors: Hennon Paul E. , Wittwer Dustin T. , Stevens John , Kilborn Ken
Published: July 2020

Live yellow-cedar (Chamaecyparis nootkatensis) trees and five classes of snags dead up to 81 yr were contrasted based on the following: the retention of bark and sapwood; the penetration of stain, decay, and weather checks; and the volume and grade of lumber recovered. Of 138 sample trees, most bark was retained on the boles of trees dead <14 yr but thereafter sloughed away and was generally missing by 51 yr after death. Stain, decay, and weather checks were limited to the narrow sapwood on snags 26 yr after tree death (n = 280 log end surfaces). Weather checking, the most serious deterioration defect in snags, did not encroach on heartwood until the sapwood was missing some 51 yr after death. Checking penetration averaged 1.3 in. for the last two snag classes. Cubic volume recovery from 305 mill-length logs did not differ significantly by classes of live trees or snags dead up to 26 yr, and the significant reduction in recovery from snags dead 51 and 81 yr was less than 15%. Grades of lumber were generally similar among the classes of trees, but no clear lumber was recovered from the oldest snag class. These results indicate little to no measurable difference of recovered wood from live trees and the first three snag classes, dead up to 26 yr, and a modest reduction in volume and grade in snags dead up to 81 yr. West. J. Appl. For. 15(2):49-58.