bluuebunny/crossref_metadata_2025 huggingface dataset:
Engaging and Empowering Malaysian Students Through Open and Distance Learning in the Post-COVID Era
Authors: Abdullah Zaleha , Mohamad Said Mohd Nihra Haruzuan
Published: May 2022

With the outbreak of COVID-19, online open and distance learning (ODL) has become increasingly relevant, particularly among those who aim to pursue postgraduate studies. ODL provides an opportunity for many to study while working or raising a family. Nevertheless, ODL programs are associated with low student engagement and high non-completion rates compared to traditional programs. Among the main contributing factors are communication and course design, which relate to the level of responsiveness of instructors and the quality of course design thus delivery through online. An innovative approach is needed to address these issues, and heutagogy seems to be a viable alternative. Nevertheless, the heutagogical approach alone is incomplete without considering the instructional scaffolding technique that can affect adult students’ engagement. This study introduces a model that combines heutagogy and instructional scaffolding (HEIS) as a guideline in conducting a fully online ODL course called Technology and Media Design. It interrogates the impact of the course design from the perspective of postgraduate students and instructors in one of Malaysia’s public universities. Recommendations include for faculties with ODL courses to continuously help develop instructors’ competencies and using more suitable assessment approaches e.g., project-based.
Testosterone Deficiency in Sickle Cell Disease: Recognition and Remediation
Authors: Musicki Biljana , Burnett Arthur L.
Published: May 2022

Hypogonadism is common in men with sickle cell disease (SCD) with prevalence rates as high as 25%. Testicular failure (primary hypogonadism) is established as the principal cause for this hormonal abnormality, although secondary hypogonadism and compensated hypogonadism have also been observed. The underlying mechanism for primary hypogonadism was elucidated in a mouse model of SCD, and involves increased NADPH oxidase-derived oxidative stress in the testis, which reduces protein expression of a steroidogenic acute regulatory protein and cholesterol transport to the mitochondria in Leydig cells. In all men including those with SCD, hypogonadism affects physical growth and development, cognition and mental health, sexual function, as well as fertility. However, it is not understood whether declines in physical, psychological, and social domains of health in SCD patients are related to low testosterone, or are consequences of other abnormalities of SCD. Priapism is one of only a few complications of SCD that has been studied in the context of hypogonadism. In this pathologic condition of prolonged penile erection in the absence of sexual excitement or stimulation, hypogonadism exacerbates already impaired endothelial nitric oxide synthase/cGMP/phosphodiesterase-5 molecular signaling in the penis. While exogenous testosterone alleviates priapism, it disadvantageously decreases intratesticular testosterone production. In contrast to treatment with exogenous testosterone, a novel approach is to target the mechanisms of testosterone deficiency in the SCD testis to drive endogenous testosterone production, which potentially decreases further oxidative stress and damage in the testis, and preserves sperm quality. Stimulation of translocator protein within the transduceosome of the testis of SCD mice reverses both hypogonadism and priapism, without affecting intratesticular testosterone production and consequently fertility. Ongoing research is needed to define and develop therapies that restore endogenous testosterone production in a physiologic, mechanism-specific fashion without affecting fertility in SCD men.
Dietary Feeding Lycopene, Citric Acid, and Chlorella Alleviated the Neurotoxicity of Polyethylene Microplastics in African Catfish (Clarias gariepinus)
Authors: Hamed Mohamed , Soliman Hamdy A. M. , Eid Zainab , Al Naggar Yahya , Sayed Alaa El-Din H.
Published: May 2022

A few studies assessed how natural products can protect fish from the neurotoxic effects of Microplastics (MPs). Therefore, the goal of this study was to look into the neurotoxicity of PE-MPs on the brain tissue of African catfish (C. gariepinus), and whether dietary feeding on Chlorella, citric acid, and lycopene could help alleviate their toxicity. Five groups of fish were used: The first group received a standard diet (control). The second group was fed 500 mg/kg PE-MP. The third group was fed PE-MP + lycopene (500 mg/kg diet). The fourth group was fed PE-MP + citric acid (30 g/kg diet). And the fifth group was fed PE-MP + Chlorella (50 g/kg diet) for 15 days. The activities of Acetylcholinesterase (Ach), Monoamine Oxidase (MAO), Aldehyde Oxidase (AO), and Nitric Oxide (NO), and the histological effect on brain tissues were then assessed. The activity of the four neurological biomarker enzymes investigated was altered significantly in fish subjected to PE-MP alone compared with the control group. For fish exposed to PE-MP with lycopene, citric acid, or Chlorella, the activities of these neurological enzymes significantly improved particularly with Chlorella compared with fish fed PE-MP individually. Histological investigations illustrated that being subjected to PE-MPs effected cellular alterations in the telencephalon, including diffuse distorted and degraded neurons, encephalomalacia, aggregated neuroglial cells (gliosis), as well as deformed and necrotic neurons, neuropil vacuolation (spongiosis), aggregated neuroglial cells (gliosis), pyknotic neurons, and shrunken Purkinje cells which were found in the cerebellum. Most histological alterations induced by exposure to PE-MP feeding were restored by dietary feeding on Chlorella, citric acid, and lycopene. Accordingly, this study recommends using citric acid, lycopene, and Chlorella as a natural remedy against MP neurotoxicity particularly with Chlorella.
Self-Guided Smartphone Excursions in University Teaching—Experiences From Exploring “Water in the City”
Authors: Schwarzenbach Franziska M. , Seibert Jan , van Meerveld H. J. (Ilja)
Published: May 2022

Like many other university teachers, we were faced with an unprecedented situation in spring 2020, when we had to cancel on-site teaching and excursions due to the Covid-19 pandemic. However, we were in the fortunate position that we had already started to develop a smartphone-based self-guided excursion on the topic of “Water in the City”. We accelerated this development and used it to replace the traditional group excursion in our Bachelor level introductory course in Hydrology and Climatology. The excursion of this course is visited by around 150 students each year. Because the student feedback was overall very positive, we used the self-guided excursion again in 2021 and plan to continue to use it in the coming years. In this paper, we describe the excursion, discuss the experiences of the students and ourselves, and present recommendations and ideas that could be useful for similar excursions at other universities.
Fast and Slow Responses of the Indian Summer Monsoon to the Direct Radiative Effect of West Asian Dust Aerosols
Authors: Wang Anqi , Xie Xiaoning , Liu Xiaodong , Shi Zhengguo
Published: May 2022

The direct radiative effect (DRE) of west Asian dust aerosols can modulate the Indian summer monsoon (ISM) through by directly heating the atmosphere (fast response) and cooling the sea surface (slow response). However, the relative contributions between fast and slow responses are unclear. In this study, the Community Atmospheric Model 4 (CAM4) with an updated Bulk Aerosol Model (BAM) has been used to identify these different responses of the ISM to the dust DRE. Our results show that the DRE of the west Asian deserts (WAD) dust significantly enhances ISM during the early monsoon season (May–June) but weakens it during the late monsoon (July–August). Further analysis shows that the fast response heats the lower troposphere over WAD and the adjacent ocean, strengthens the southwesterly flows over the Arabian Sea, and thus increases the precipitation in central and eastern India (CEI), which dominates the ISM enhancement during the early monsoon season. During the late monsoon season, the slow response cools the sea surface temperature (SST) and the lower troposphere over Arabian Sea, creates the easterly flows to inhibit the moisture transport and decreases the CEI precipitation, ultimately weakening the ISM. Additionally, observational evidence of the trends in the WAD and CEI precipitation from 1980 to 2015 supports our simulated results. Our results suggest that the dust DRE induced ocean feedback plays a non-negligible role in modulating cross-seasonal climate effect of dust aerosols.
Long-Term Shifts in Faunal Composition of Freshwater Mollusks in Spring-Fed Rivers of Florida
Authors: Kusnerik Kristopher M. , Means Guy H. , Portell Roger W. , Kannai Alshina , Monroe Mariah M. , Means Ryan , Kowalewski Michal
Published: May 2022

Florida’s freshwater spring and river ecosystems have been deteriorating due to direct and indirect human impacts. However, while the conservation and restoration strategies employed to mitigate these effects often rely on faunal surveys that go back several decades, the local ecosystem shifts tend to have much deeper roots that predate those faunal surveys by centuries or millennia. Conservation paleobiology, an approach which enhances our understanding of the past states of ecosystems, allows for comparison of modern faunal communities with those prior to significant human impacts. This study examines the historical record of freshwater mollusk assemblages from two spring-fed river systems, the Wakulla and Silver/Ocklawaha Rivers. Specifically, we compared fossil assemblages (latest Pleistocene - early Holocene) and live mollusk assemblages in the two targeted river systems. Bulk sampling of the fossil record (20 sites; 70 samples; 16,314 specimens) documented relatively diverse mollusk assemblages that consist of a suite of native freshwater species that is similar across the studied systems. In contrast, sampling of live communities (24 sites; 138 samples; 7,572 specimens) revealed depauperate species assemblies characterized by the absence of multiple native freshwater species commonly found in fossil samples, the widespread presence of introduced species, and dominance of brackish-tolerant species at the lower Wakulla River sites. Unlike fossil mollusk assemblages, live mollusk assemblages differ notably between the two river systems due to differences in relative abundance of introduced species (Melanoides tuberculataandCorbicula fluminea) and the presence of brackish-tolerant mollusks in the coastally influenced Wakulla River. The diverse, exclusively freshwater mollusk associations comparable across multiple river systems documented in the fossil record provide a historical perspective on the past state of freshwater river ecosystems complementing data provided by modern surveys. The conservation paleobiology approach used in this study reinforces the importance of considering the historical ecology of an ecosystem and the utility of the fossil record in providing a historical perspective on long-term faunal changes.
NISC: Neural Network-Imputation for Single-Cell RNA Sequencing and Cell Type Clustering
Authors: Zhang Xiang , Chen Zhuo , Bhadani Rahul , Cao Siyang , Lu Meng , Lytal Nicholas , Chen Yin , An Lingling
Published: May 2022

Single-cell RNA sequencing (scRNA-seq) reveals the transcriptome diversity in heterogeneous cell populations as it allows researchers to study gene expression at single-cell resolution. The latest advances in scRNA-seq technology have made it possible to profile tens of thousands of individual cells simultaneously. However, the technology also increases the number of missing values, i. e, dropouts, from technical constraints, such as amplification failure during the reverse transcription step. The resulting sparsity of scRNA-seq count data can be very high, with greater than 90% of data entries being zeros, which becomes an obstacle for clustering cell types. Current imputation methods are not robust in the case of high sparsity. In this study, we develop a Neural Network-based Imputation for scRNA-seq count data, NISC. It uses autoencoder, coupled with a weighted loss function and regularization, to correct the dropouts in scRNA-seq count data. A systematic evaluation shows that NISC is an effective imputation approach for handling sparse scRNA-seq count data, and its performance surpasses existing imputation methods in cell type identification.
A Panel of E2F Target Gene Signature Predicting the Prognosis of Hepatocellular Carcinoma
Authors: Hu Wenmin , Shi Yongmei , Han Tongqin , Liu Caiyun , Cao Xipeng , Shi Guangjun , Zhu Wenjing
Published: May 2022

Hepatocellular carcinoma is one of the most malignant tumors, and the therapeutic effects of traditional treatments are poor. It is urgent to explore and identify new biomarkers and therapeutic targets to develop novel treatments which are individualized and effective. Three hallmarks, including E2F targets, G2M checkpoint and DNA repair, were collected by GSEA analysis. The panel of E2F-related gene signature consisted of five genes: HN1, KIF4A, CDCA3, CDCA8 and SSRP1. They had various mutation rates ranging from 0.8 to 5% in hepatocellular carcinoma, and patients with gene mutation had poorer prognosis. Among these genes, HN1 has the greatest mutation rate, and SSRP1 has the greatest impact on the model with a B (COX) value of 0.8842. Patients with higher expression of these genes had poorer prognosis. Kaplan-Meier curves in stratified survival analysis confirmed that patients with high risk scores had poor prognosis (p < 0.05). The results of univariate and multivariate COX survival analysis showed that risk score was closely related to the overall survival of patients with hepatocellular carcinoma. For clinical validation, we found that all the genes in the model were upregulated in hepatocellular carcinoma tissues compared to normal liver tissues, which was consistent with the previous results we obtained. Our study demonstrated that a panel of E2F target genes signature including five genes could predict the prognosis of hepatocellular carcinoma. This panel gene signature can facilitate the development of individualized and effective treatment for hepatocellular carcinoma.
Pandemic, Epidemic, Endemic: B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus
Authors: Stewart Alexander , Sinclair Emma , Ng Joseph Chi-Fung , O’Hare Joselli Silva , Page Audrey , Serangeli Ilaria , Margreitter Christian , Orsenigo Federica , Longman Katherine , Frampas Cecile , Costa Catia , Lewis Holly-May , Kasar Nora , Wu Bryan , Kipling David , Openshaw Peter JM , Chiu Christopher , Baillie J Kenneth , Scott Janet T. , Semple Malcolm G. , Bailey Melanie J. , Fraternali Franca , Dunn-Walters Deborah K.
Published: May 2022

Immunoglobulin gene heterogeneity reflects the diversity and focus of the humoral immune response towards different infections, enabling inference of B cell development processes. Detailed compositional and lineage analysis of long read IGH repertoire sequencing, combining examples of pandemic, epidemic and endemic viral infections with control and vaccination samples, demonstrates general responses including increased use of IGHV4-39 in both Zaire Ebolavirus (EBOV) and COVID-19 patient cohorts. We also show unique characteristics absent in Respiratory Syncytial Virus or yellow fever vaccine samples: EBOV survivors show unprecedented high levels of class switching events while COVID-19 repertoires from acute disease appear underdeveloped. Despite the high levels of clonal expansion in COVID-19 IgG1 repertoires there is a striking lack of evidence of germinal centre mutation and selection. Given the differences in COVID-19 morbidity and mortality with age, it is also pertinent that we find significant differences in repertoire characteristics between young and old patients. Our data supports the hypothesis that a primary viral challenge can result in a strong but immature humoral response where failures in selection of the repertoire risk off-target effects.
Neutrophil Count Predicts Malignant Cerebellar Edema and Poor Outcome in Acute Basilar Artery Occlusion Receiving Endovascular Treatment: A Nationwide Registry-Based Study
Authors: Liu Chang , Li Fengli , Liu Shuai , Chen Qiong , Sang Hongfei , Yang Qingwu , Zhou Kai , Zi Wenji
Published: May 2022

Background

Acute basilar artery occlusion (ABAO) is known to have a poor outcome with a high rate of morbidity and mortality despite endovascular treatment (EVT), highlighting the necessities of exploring factors to limit the efficacy of EVT in these patients. Cerebellar infarctions in ABAO might progress to malignant cerebellar edema (MCE), a life-threatening complication after reperfusion, posing a secondary injury to the brainstem by mass effects. Therefore, the present research aimed to explore the impacts of MCE on a long-term outcome and investigate the prognostic factors for MCE among ABAO after EVT.

Methods

In the national BASILAR registry, a total of 329 ABO patients with cerebellar infarctions treated by EVT met the inclusion criteria. The presence of MCE defined by the Jauss scale ≥4 points, was evaluated on the computed tomography performed 72 h after EVT. The adjusted odds ratio and 95% CI were obtained by logistic regression models. A favorable outcome was defined as a 90-day modified Rankin Scale score of 0–3.

Results

MCE was statistically associated with the decreased incidence of a favorable outcome [adjusted odds ratio, 0.35(95% CI, 0.18-0.68), P=0.002]. The baseline National Institutes of Health Stroke Scale score, collateral circulation, neutrophil count at admission, and recanalization status were predictors for MCE and a favorable functional status at 90 days (all P<0.05). Among all inflammatory factors, the neutrophil count achieved the highest accuracy, sensitivity, and specificity for MCE. Adding the neutrophil count status into the baseline model obviously enhanced its prediction ability for MCE and favorable outcome by increasing the area under curve and achieving both net reclassification and integrated discrimination improvement (all P<0.05). Mediation analysis indicated that MCE mediated the association between the increased neutrophil count and worse functional outcome (P=0.026).

Discussion

MCE acted essential roles in worsening prognosis for ABAO after EVT. A high neutrophil count at admission was linked to MCE and a poor outcome among ABAO patients, which could be further incorporated into the clinical decision-making system and guide immunomodulation therapy.
Adult Vaccination as a Protective Factor for Dementia: A Meta-Analysis and Systematic Review of Population-Based Observational Studies
Authors: Wu Xinhui , Yang Haixia , He Sixian , Xia Ting , Chen Diang , Zhou Yexin , Liu Jin , Liu MengSi , Sun Zhen
Published: May 2022

Background

Common vaccinations may have impacts on dementia risk, but current evidence is inconsistent. We therefore investigated the association between vaccinations and dementia risk by systematic review and meta-analysis approach.

Methods

We conducted an extensive search of PubMed, Embase, Cochrane Library, and Web of Science to identify studies that compared the risk of dementia in vaccinated versus unvaccinated populations. The adjusted hazard ratio (HR) and corresponding 95% confidence intervals (CIs) were pooled as measures.

Results

Of the 9124 records initially retrieved, 17 studies with 1857134 participants were included in our analysis. The overall pooled results showed that vaccinations were associated with a 35% lower dementia risk (HR=0.65, 95% CI: 0.60-0.71, Poverall effect < 0.001; I2 =91.8%, Pheterogeneity<0.001). All types of vaccination were associated with a trend toward reduced dementia risk, with rabies (HR=0.43), tetanus & diphtheria & pertussis (Tdap) (HR=0.69), herpes zoster (HR=0.69), influenza (HR=0.74), hepatitis A (HR=0.78), typhoid (HR=0.80), and hepatitis B (HR=0.82) vaccinations being significant. Individuals with more full vaccination types and more annual influenza vaccinations were less likely to develop dementia. Gender and age had no effect on this association.

Conclusion

Routine adult vaccinations are associated with a significant reduction in dementia risk and may be an effective strategy for dementia prevention. Further research is needed to elucidate the causal effects of this association and the underlying mechanisms.
PRKAR1A and SDCBP Serve as Potential Predictors of Heart Failure Following Acute Myocardial Infarction
Authors: Chen Qixin , Su Lina , Liu Chuanfen , Gao Fu , Chen Hong , Yin Qijin , Li Sufang
Published: May 2022

Background and Objectives

Early diagnosis of patients with acute myocardial infarction (AMI) who are at a high risk of heart failure (HF) progression remains controversial. This study aimed at identifying new predictive biomarkers of post-AMI HF and at revealing the pathogenesis of HF involving these marker genes.

Methods and Results

A transcriptomic dataset of whole blood cells from AMI patients with HF progression (post-AMI HF, n = 16) and without progression (post-AMI non-HF, n = 16) was analyzed using the weighted gene co-expression network analysis (WGCNA). The results indicated that one module consisting of 720 hub genes was significantly correlated with post-AMI HF. The hub genes were validated in another transcriptomic dataset of peripheral blood mononuclear cells (post-AMI HF, n = 9; post-AMI non-HF, n = 8). PRKAR1A, SDCBP, SPRED2, and VAMP3 were upregulated in the two datasets. Based on a single-cell RNA sequencing dataset of leukocytes from heart tissues of normal and infarcted mice, PRKAR1A was further verified to be upregulated in monocytes/macrophages on day 2, while SDCBP was highly expressed in neutrophils on day 2 and in monocytes/macrophages on day 3 after AMI. Cell–cell communication analysis via the “CellChat” package showed that, based on the interaction of ligand–receptor (L–R) pairs, there were increased autocrine/paracrine cross-talk networks of monocytes/macrophages and neutrophils in the acute stage of MI. Functional enrichment analysis of the abovementioned L–R genes together with PRKAR1A and SDCBP performed through the Metascape platform suggested that PRKAR1A and SDCBP were mainly involved in inflammation, apoptosis, and angiogenesis. The receiver operating characteristic (ROC) curve analysis demonstrated that PRKAR1A and SDCBP, as well as their combination, had a promising prognostic value in the identification of AMI patients who were at a high risk of HF progression.

Conclusion

This study identified that PRKAR1A and SDCBP may serve as novel biomarkers for the early diagnosis of post-AMI HF and also revealed their potentially regulatory mechanism during HF progression.
Killing Two Birds With One Stone: Effective Control of Both Non-Small Cell Lung Cancer and Progressive Multifocal Leukoencephalopathy With Atezolizumab, A Case Report
Authors: Lambert Nicolas , El Moussaoui Majdouline , Ritacco Caroline , Moïse Martin , Paulus Astrid , Delvenne Philippe , Baron Frédéric , Sadzot Bernard , Maquet Pierre
Published: May 2022

Treating patients with cancer complicated by severe opportunistic infections is particularly challenging since classical cancer treatments, such as chemotherapy, often induce profound immune suppression and, as a result, may favor infection progression. Little is known about the potential place of immune checkpoint inhibitors in these complex situations. Here, we report a 66-year-old man who was concomitantly diagnosed with non-small cell lung cancer and progressive multifocal leukoencephalopathy. The patient was treated with anti-PD-L1 antibody atezolizumab, which allowed effective control of both lung cancer and progressive multifocal leukoencephalopathy, as demonstrated by the patient’s remarkable neurologic clinical improvement, JC viral load reduction in his cerebrospinal fluid, regression of the brain lesions visualized through MRI, and the strict radiological stability of his cancer. In parallel, treatment with atezolizumab was associated with biological evidence of T-cell reinvigoration. Hence, our data suggest that immune checkpoint inhibitors may constitute a treatment option for patients with cancer complicated by severe opportunistic infections.
Potential Impact of Late 1980s Regime Shift on the Collapse of Walleye Pollock Catch in the Western East/Japan Sea
Authors: Kim Yong-Yub , Kang Yu-Kyeong , Lee Seung-Tae , Jung Hae Kun , Lee Chung Il , Kim Sangil , Jeong Kwang Young , Byun Do-Seong , Cho Yang-Ki
Published: May 2022

Walleye pollock (Gadus chalcogramma) caught in the Korean fishing area dramatically decreased in the late 1980s. To investigate the potential impact of the late 1980s climate regime shift on the collapse of the pollock catch, we developed a three-dimensional hydrodynamic model with data assimilation and a particle tracking model. Data-assimilated reanalysis showed that sea surface temperature increased by approximately 2°C in the spawning area of pollock in the late 1980s. The suitable spawning area in the East Korean Bay decreased due to warming in the late 1980s. Spawned eggs of walleye pollock were tracked using a particle tracking model for 30 days in January and February during 1983–1992. The number of individuals transported to the nursery within the Korean fishing area from the spawning area was reduced by 74% in the late 1980s. The intensified East Korean Warm Current (EKWC) could be responsible for the decreased number of individuals transported to the southern area in the late 1980s. Warming in the Korean fishing area could also cause a decrease in pollock. These oceanic changes might be linked to climate regime shifts in the late 1980s. The warming regime with positive Arctic Oscillation and weakened monsoon intensified the northward flow of the EKWC and accelerated the warming of the spawning and fishing areas in the late 1980s.
To Regulate or Not to Regulate: Assimilation of Dietary Fatty Acids in the Temperate Copepod Temora longicornis
Authors: Franco-Santos Rita M. , Auel Holger , Boersma Maarten , De Troch Marleen , Graeve Martin , Meunier Cédric Leo , Niehoff Barbara
Published: May 2022

Consumer regulation of lipid composition during assimilation of dietary items is related to their ecology, habitat, and life cycle, and may lead to extra energetic costs associated with the conversion of dietary material into the fatty acids (FAs) necessary to meet metabolic requirements. For example, lipid-rich copepods from temperate and polar latitudes must convert assimilated dietary FAs into wax esters, an efficient type of energy storage which enables them to cope with seasonal food shortages and buoyancy requirements. Lipid-poor copepods, however, tend to not be as constrained by food availability as their lipid-rich counterparts and, thus, should have no need for modifying dietary FAs. Our objective was to test the assumption thatTemora longicornis, a proxy species for lipid-poor copepods, does not regulate its lipid composition. Isotopically-enriched (13C) diatoms were fed to copepods during a 5-day laboratory experiment. Compound-specific stable isotope analysis of algae and copepod samples was performed in order to calculate dietary FA assimilation, turnover, and assimilation efficiency into copepod FAs. Approximately 65% of the total dietary lipid carbon (C) assimilated (913 ± 68 ng C ind-1at the end of the experiment) was recorded as polyunsaturated FAs, with 20 and 15% recorded as saturated and monounsaturated FAs, respectively. As expected,T. longicornisassimilated dietary FAs in an unregulated, non-homeostatic manner, as evidenced by the changes in its FA profile, which became more similar to that of their diet. Copepods assimilated 11% of the total dietary C (or 40% of the dietary lipid C) ingested in the first two days of the experiment. In addition, 34% of their somatic growth (in C) after two days was due to the assimilation of dietary C in FAs. Global warming may lead to increased proportions of smaller copepods in the oceans, and to a lower availability of algae-produced essential FAs. In order for changes in the energy transfer in marine food webs to be better understood, it is important that future investigations assess a broader range of diets as well as lipid-poor zooplankton from oceanographic areas throughout the world’s oceans.
First Autonomous Underwater Vehicle Observations of a Potential Petrale Sole (Eopsetta jordani) Spawning Aggregation Off the US West Coast
Authors: Powell Abigail , Clarke M. Elizabeth , Haltuch Melissa A. , Fruh Erica , Anderson Jeffrey , Whitmire Curt E. , Johnson Melanie M.
Published: May 2022

Understanding the timing and reproductive behavior of commercial fish species is a key part of well-informed stock assessments and fishery management, but this information is often limited, particularly for species that spawn in deep water. Petrale sole (Eopsetta jordani) is one of the most commercially important flatfish species in the US and is known to spawn off the West Coast during winter months. A number of spawning grounds have been identified using catch data and tagging studies, but to our knowledge there have been no direct visual observations of aggregating petrale sole. In 2018, we observed unusually high densities of petrale sole in autonomous underwater vehicle (AUV) imagery collected at a feature inshore of Santa Lucia Bank off the California coast. In addition to aggregations of fish, we observed fish in positions and configurations that we believe to be related to spawning behavior including physical contact between individuals and evidence of consistent size differentiation within clusters. We present images of the aggregating petrale sole and information on the physical and environmental conditions at this site. We also compare these observations to the results of AUV surveys carried out in 2005 at the same site. Analysis of commercial catch data from this area supports the hypothesis that this is a seasonal petrale sole aggregation as fishing at this location occurs mainly during winter months with catches dominated by petrale sole. In addition to the potential identification of a spawning aggregation, these observations show how advanced technologies can provide insight into the reproductive behavior of a commercially important species in-situ.
Abundance and Diversity of Dimethylsulfoniopropionate Degradation Genes of Roseobacter Group in the Northern South China Sea
Authors: Sun Fulin , Wang Youshao , Jiang Zhaoyu , Sun Cuici , Wang Yutu , Wu Meilin
Published: May 2022

Bacterial degradation of dimethylsulfoniopropionate (DMSP) plays a significant role in ecosystem productivity and global climate. In this study, the abundance and diversity of Roseobacter group DMSP degradation genes were explored in spatial scale of the South China Sea (SCS). Quantitative PCR showed that a higher abundance of dmdA (DMSP demethylase) and dddP (DMSP lyase) genes was detected above 75 m than deep water, especially in surface water. A high ratio of dmdA/dddP existed in all sites and increased with water depth, indicating that demethylation was the main degradation pathway in the Roseobacter group. High-throughput sequencing analysis showed that distribution of dmdA gene had a significant layering structure in the northern SCS, and high taxonomic diversity of dmdA gene was observed in near-surface waters (25 and 50 m). DmdA gene in the Roseobacter group, such as Leisingera, Nioella, Roseobacter, Roseovarius, Donghicola, Phaeobacter, and Tateyamaria, had remarkable specificity due to the effect of different sites and water depths. Different ecological strategies of DMSP degradation may be used by members of the bacterial community harboring demethylation genes. In addition, many dmdA sequences were affiliated with unidentified bacteria, indicating that the SCS reserved high diversity of DMSP-degrading bacteria. Canonical correspondence analysis (CCA) suggested that temperature and depth were the most important factors to determine the taxonomic distribution of DMSP degradation genes in the Roseobacter group, as well as their abundance. This study highlighted the understanding of the role of Roseobacter group in DMSP degradation in the tropical ocean.
Study on the Variation Pattern and Influencing Factors of Poisson’s Ratio of Bamboo
Authors: Lu Haojie , Lian Hongyang , Xu Jiayu , Ma Niuniu , Zhou Zhezhe , Song Yuanpu , Yu Youming , Zhang Xiaochun
Published: May 2022

As a natural polymer material, bamboo is regarded as a super material with lightweight but excellent mechanical properties. With the increasing application of bamboo in structures, high-strength composite materials, bridges, musical instruments, and damping materials, the study of Poisson’s ratio is of great significance to the structural design and stability of these materials. In this work, Poisson’s ratio of bamboo for different ages, growth heights, and bamboo wall radial positions were determined by electrical measurement. The relationships between intrinsic factors (density, chemical composition, crystallinity, and microfibril angle) and Poisson’s ratio were also analyzed. The results showed that Poisson’s ratio of bamboo for different ages and locations ranged from 0.180 to 0.334, decreased with the increasing bamboo age, growth height, and the radial position from the outer layer to the inner layer. While the peak values of Poisson’s ratio were obtained at bamboo of 2 years old, lower growth height, and near the outer layer of the bamboo. Furthermore, the Poisson’s ratio had a positive correlation with the increasing density, cellulose content, and MFA, but a negative correlation with lignin content. There is no direct relationship between the crystallinity and Poisson’s ratio, but it affects the axial and transverse deformation of bamboo, which can be used as an indirect index to judge Poisson’s ratio.
Trends in Influenza Vaccination Rates in Participants With Airflow Limitation: The Korea National Health and Nutrition Examination Survey 2007–2018
Authors: Lee Hyun , Choi Hayoung , Jo Yong Suk
Published: May 2022

Background

Influenza vaccination is strongly recommended for people with chronic lung diseases, including chronic obstructive pulmonary disease, to reduce risk of exacerbation. We assess the influenza vaccination rate and its related factors in participants with airflow limitation (AFL) using nationally representative data in Korea.

Methods

We conducted a cross-sectional study from the Korea National Health and Nutrition Examination Survey from 2007 to 2018. Individuals ≥ 40 years who underwent spirometry and had identifiable information on influenza vaccination status were included.

Results

Overall influenza vaccination coverage was 61.2% in participants with AFL and 41.8% in participants without AFL. Age had a significant impact on the yearly vaccination rate in participants with AFL. Over the 10 years of study period, while the yearly vaccination rate steadily increased from 58.3 to 61.9% in elderly participants (≥65 years) with AFL (p for trend = 0.117), the yearly vaccination rate decreased from 41.5% to 30.8% in younger participants (<65 years) (p for trend = 0.038). In multivariable analyses, younger age [adjusted odds ratio (OR) for unvaccinated = 0.88, 95% confidence interval (CI) = 0.87–0.90], male (adjusted OR = 1.64; 95% CI = 1.23–2.19), and current smokers (adjusted OR = 1.42, 95% CI = 1.01–2.00) were associated with increased odds of being unvaccinated.

Conclusions

The vaccination rate in participants with AFL affected by age. Younger age, male sex, and current smoking were associated with unvaccinated status. More attention and targeted interventions are required to improve the influenza vaccination rate in those with AFL.
Immunotherapy in Penile Squamous Cell Carcinoma: Present or Future? Multi-Target Analysis of Programmed Cell Death Ligand 1 Expression and Microsatellite Instability
Authors: Montella Marco , Sabetta Rosalaura , Ronchi Andrea , De Sio Marco , Arcaniolo Davide , De Vita Ferdinando , Tirino Giuseppe , Caputo Alessandro , D’Antonio Antonio , Fiorentino Francesco , Facchini Gaetano , Lauro Giovanni Di , Perdonà Sisto , Ventriglia Jole , Aquino Gabriella , Feroce Florinda , Borges Dos Reis Rodolfo , Neder Luciano , Brunelli Matteo , Franco Renato , Zito Marino Federica
Published: May 2022

Background

Penile cancer (PC) is an extremely rare malignancy, and the patients at advanced stages have currently limited treatment options with disappointing results. Immune checkpoint inhibitors anti-programmed cell death 1 (PD-1)/programmed cell death ligand 1 (PD-L1) are currently changing the treatment of several tumors. Furthermore, the microsatellite instability (MSI) and the deficient mismatch repair system (dMMR) proteins represent predictive biomarkers for response to immune checkpoint therapy. Until present, few data have been reported related to PD-L1 expression and MSI in PC. The main aim of our study was the evaluation of PD-L1 expression in tumor cells (TCs) and tumor-infiltrating lymphocytes (TILs) in immune cells and the analysis of dMMR/MSI status in a large series of PCs.

Methods

A series of 72 PC, including 65 usual squamous cell carcinoma (USCC), 1 verrucous, 4 basaloid, 1 warty, and 1 mixed (warty-basaloid), was collected. Immunohistochemistry (IHC) was performed to assess PD-L1 expression using two different anti-PD-L1 antibodies (clone SP263 and SP142 Ventana) and MMR proteins expression using anti-MLH1, anti-PMS2, anti-MSH2, and anti-MSH6 antibodies. PCR analysis was performed for the detection of MSI status.

Results

Of the 72 PC cases analyzed by IHC, 45 (62.5%) cases were TC positive and 57 (79%) cases were combined positive score (CPS) using PDL1 SP263. In our cohort, TILs were present in 62 out of 72 cases (86.1%), 47 (75.8%) out of 62 cases showed positivity to PDL1 clone SP142. In our series, 59 cases (82%) had pMMR, 12 cases (16.7%) had lo-paMMR, and only 1 case (1.3%) had MMR. PCR results showed that only one case lo-paMMR was MSI-H, and the case dMMR by IHC not confirmed MSI status.

Conclusion

Our findings showed that PD-L1 expression and MSI status represent frequent biological events in this tumor suggesting a rationale for a new frontier in the treatment of patients with PC based on the immune checkpoint inhibitors.
The Effect of Pregabalin on the Minimum Alveolar Concentration of Sevoflurane: A Randomized, Placebo-Controlled, Double-Blind Clinical Trial
Authors: Müller Johannes , Plöchl Walter , Mühlbacher Paul , Graf Alexandra , Stimpfl Thomas , Hamp Thomas
Published: May 2022

Background

Pregabalin is commonly used perioperatively to reduce post-operative pain and opioid consumption and to prevent the development of chronic pain. It has been shown to reduce anesthetic consumption in balanced anesthesia, but studies investigating its effect on the minimum alveolar concentration (MAC) of volatile anesthetics are lacking. The aim of this study was to investigate the effect of two different doses of pregabalin on the MAC of sevoflurane.

Methods

In a randomized, double-blinded, placebo controlled clinical study, 75 patients were assigned to receive placebo, 300 mg pregabalin, or 150 mg pregabalin, as a capsule 1 h before anesthesia induction with sevoflurane only. After equilibration, the response to skin incision (movement vs. non-movement) was monitored. The MAC was assessed using an up- and down-titration method.

Results

The MAC of sevoflurane was estimated as 2.16% (95% CI, 2.07–2.32%) in the placebo group, 1.44% (95% CI, 1.26–1.70%) in the 300 mg pregabalin group, and 1.81% (95% CI, 1.49–2.13%) in the 150 mg pregabalin group. We therefore report a 33% reduction in the MAC of sevoflurane in the 300 mg pregabalin group as compared to placebo. The MAC of the 150 mg pregabalin group was reduced by 16% as compared to placebo but was not statistically significant.

Conclusions

The administration of 300 mg pregabalin reduced the MAC of sevoflurane by 33%, while the administration of 150 mg pregabalin did not significantly reduce the MAC of sevoflurane. Pregabalin use led to a small reduction in post-operative pain levels but increased side effects in a dose-dependent manner.
A 6-Year Update on the Diversity of Methicillin-Resistant Staphylococcus aureus Clones in Africa: A Systematic Review
Authors: Lawal Opeyemi Uwangbaoje , Ayobami Olaniyi , Abouelfetouh Alaa , Mourabit Nadira , Kaba Mamadou , Egyir Beverly , Abdulgader Shima M. , Shittu Adebayo Osagie
Published: May 2022

Background

Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of hospital-associated (HA) and community-associated (CA) infections globally. The multi-drug resistant nature of this pathogen and its capacity to cause outbreaks in hospital and community settings highlight the need for effective interventions, including its surveillance for prevention and control. This study provides an update on the clonal distribution of MRSA in Africa.

Methods

A systematic review was conducted by screening for eligible English, French, and Arabic articles from November 2014 to December 2020, using six electronic databases (PubMed, EBSCOhost, Web of Science, Scopus, African Journals Online, and Google Scholar). Data were retrieved and analyzed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis guidelines (registered at PROSPERO: CRD42021277238). Genotyping data was based primarily on multilocus sequence types (STs) and Staphylococcal Cassette Chromosome mec (SCCmec) types. We utilized the Phyloviz algorithm in the cluster analysis and categorization of the MRSA STs into various clonal complexes (CCs).

Results

We identified 65 studies and 26 publications from 16 of 54 (30%) African countries that provided sufficient genotyping data. MRSA with diverse staphylococcal protein A (spa) and SCCmec types in CC5 and CC8 were reported across the continent. The ST5-IV [2B] and ST8-IV [2B] were dominant clones in Angola and the Democratic Republic of Congo (DRC), respectively. Also, ST88-IV [2B] was widely distributed across the continent, particularly in three Portuguese-speaking countries (Angola, Cape Verde, and São Tomé and Príncipe). The ST80-IV [2B] was described in Algeria and Egypt, while the HA-ST239/ST241-III [3A] was only identified in Egypt, Ghana, Kenya, and South Africa. ST152-MRSA was documented in the DRC, Kenya, Nigeria, and South Africa. Panton–Valentine leukocidin (PVL)-positive MRSA was observed in several CCs across the continent. The median prevalence of PVL-positive MRSA was 33% (ranged from 0 to 77%; n = 15).

Conclusion

We observed an increase in the distribution of ST1, ST22, and ST152, but a decline of ST239/241 in Africa. Data on MRSA clones in Africa is still limited. There is a need to strengthen genomic surveillance capacity based on a “One-Health” strategy to prevent and control MRSA in Africa.
Bacteria Community Inhabiting Heterobasidion Fruiting Body and Associated Wood of Different Decay Classes
Authors: Ren Wenzi , Penttilä Reijo , Kasanen Risto , Asiegbu Fred O.
Published: May 2022

The microbiome of Heterobasidion-induced wood decay of living trees has been previously studied; however, less is known about the bacteria biota of its perennial fruiting body and the adhering wood tissue. In this study, we investigated the bacteria biota of the Heterobasidion fruiting body and its adhering deadwood. Out of 7,462 operational taxonomic units (OTUs), about 5,918 OTUs were obtained from the fruiting body and 5,469 OTUs were obtained from the associated dead wood. Interestingly, an average of 52.6% of bacteria biota in the fruiting body was shared with the associated dead wood. The overall and unique OTUs had trends of decreasing from decay classes 1 to 3 but increasing in decay class 4. The fruiting body had the highest overall and unique OTUs number in the fourth decay class, whereas wood had the highest OTU in decay class 1. Sphingomonas spp. was significantly higher in the fruiting body, and phylum Firmicutes was more dominant in wood tissue. The FAPROTAX functional structure analysis revealed nutrition, energy, degradation, and plant-pathogen-related functions of the communities. Our results also showed that bacteria communities in both substrates experienced a process of a new community reconstruction through the various decay stages. The process was not synchronic in the two substrates, but the community structures and functions were well-differentiated in the final decay class. The bacteria community was highly dynamic; the microbiota activeness, community stability, and functions changed with the decay process. The third decay class was an important turning point for community restructuring. Host properties, environmental factors, and microbial interactions jointly influenced the final community structure. Bacteria community in the fruiting body attached to the living standing tree was suppressed compared with those associated with dead wood. Bacteria appear to spread from wood tissue of the standing living tree to the fruiting body, but after the tree is killed, bacteria moved from fruiting body to wood. It is most likely that some of the resident endophytic bacteria within the fruiting body are either parasitic, depending on it for their nutrition, or are mutualistic symbionts.
Role of Insect Gut Microbiota in Pesticide Degradation: A Review
Authors: Siddiqui Junaid Ali , Khan Muhammad Musa , Bamisile Bamisope Steve , Hafeez Muhammad , Qasim Muhammad , Rasheed Muhammad Tariq , Rasheed Muhammad Atif , Ahmad Sajjad , Shahid Muhammad Ibrahim , Xu Yijuan
Published: May 2022

Insect pests cause significant agricultural and economic losses to crops worldwide due to their destructive activities. Pesticides are designed to be poisonous and are intentionally released into the environment to combat the menace caused by these noxious pests. To survive, these insects can resist toxic substances introduced by humans in the form of pesticides. According to recent findings, microbes that live in insect as symbionts have recently been found to protect their hosts against toxins. Symbioses that have been formed are between the pests and various microbes, a defensive mechanism against pathogens and pesticides. Insects’ guts provide unique conditions for microbial colonization, and resident bacteria can deliver numerous benefits to their hosts. Insects vary significantly in their reliance on gut microbes for basic functions. Insect digestive tracts are very different in shape and chemical properties, which have a big impact on the structure and composition of the microbial community. Insect gut microbiota has been found to contribute to feeding, parasite and pathogen protection, immune response modulation, and pesticide breakdown. The current review will examine the roles of gut microbiota in pesticide detoxification and the mechanisms behind the development of resistance in insects to various pesticides. To better understand the detoxifying microbiota in agriculturally significant pest insects, we provided comprehensive information regarding the role of gut microbiota in the detoxification of pesticides.