bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors for Early Hydrocephalus on Post Unilateral Thalamic Tumor Resection
Authors: Zhang Linpeng , Wang Chen , Zeng Xianwei
Published: April 2022

Objective

The outcome of surgical treatment for thalamic tumors is poor. Hydrocephalus is one of the most frequent postoperative complications after unilateral thalamic tumor resection. This study examined the relationship between surgical approaches, pathological grade, image characteristics, preoperative complications, extent of resection, and incidence of postoperative hydrocephalus.

Methods

The study retrospectively reviewed clinical data from 80 patients who underwent resection of thalamic tumors between 2015 and 2021. Data on patient survival and disease progression status were obtained retrospectively to calculate overall survival (OS) and progression free survival (PFS).

Results

No patients died during the perioperative period and two patients suffered postoperative coma. Tumors were totally resected in 44 cases (55 %), subtotally resected in 21 cases (26.25 %), and partially resected in 15 cases (18.75 %). Thirty-five cases of hydrocephalus occurred within 1 month after operation(43.75%). Surgical approaches associated with hydrocephalus were as follows: hydrocephalus occurred in seven cases after trans-frontal lateral ventricle approach for tumor resection (62.9%), in 17 cases after through parieto-occipital transventricular approach tumor resection (43.58%), and in one case after trans-frontal lateral ventricle approach for tumor resection + third ventriculostomy (7.1%). Postoperative muscle strength decrease occurred in 41 patients (51.25%). Longer PFS and OS were correlated with degree of resection in patients with thalamic glioblastoma (P < 0.05) and had no relationship with hydrocephalus.

Conclusion

Surgical treatment of thalamic tumors is an effective therapeutic method. The incidence of postoperative hydrocephalus is not associated with tumor size, degree of tumor enhancement, peritumoral edema, tumor invasion, midline crossing, and pathological grade. The incidence of postoperative hydrocephalus was higher in patients with preoperative hydrocephalus and low resection degree, and lower in patients with endoscopic third ventriculostomy. The risk of early postoperative hydrocephalus in thalamic tumors is high. Intraoperative third ventriculostomy could reduce the incidence of early postoperative hydrocephalus. PFS and OS were longer in patients with thalamic glioblastoma with a high resection degree (P < 0.05) and were not associated with hydrocephalus.
Procyanidin B2 Protects Aged Oocytes Against Meiotic Defects Through Cortical Tension Modulation
Authors: Zhuan Qingrui , Li Jun , Zhou Guizhen , Du Xingzhu , Liu Hongyu , Hou Yunpeng , Wan Pengcheng , Fu Xiangwei
Published: April 2022

Defects in meiotic process are the main factors responsible for the decreased developmental competence in aged oocytes. Our recent research indicated that natural antioxidant procyanidin B2 (PCB2) promoted maturation progress in oocytes from diabetic mice. However, the effect of PCB2 on aging-induced chromosome abnormalities and the underlying mechanism have not been explored. Here, we found that PCB2 recovered aging-caused developmental arrest during meiotic maturation, germinal vesicle breakdown (GVBD) rate was significantly higher in aged oocytes treated with PCB2 (P < 0.05). Furthermore, we discovered that cortical mechanics were altered during aging process, cortical tension-related proteins were aberrantly expressed in aged oocytes (P < 0.001). PCB2 supplementation efficaciously antagonized aging-induced decreased cortical tension (P < 0.001). Moreover, PCB2 restored spindle morphology (P < 0.01), maintained proper chromosome alignment (P < 0.05), and dramatically reduced reactive oxygen species (ROS) level (P < 0.05) in aged oocytes. Collectively, our results reveal that PCB2 supplementation is a feasible approach to protect oocytes from reproductive aging, contributing to the improvement of oocytes quality.
Senecavirus A Entry Into Host Cells Is Dependent on the Cholesterol-Mediated Endocytic Pathway
Authors: Jia Meiyu , Sun Mingxia , Tang Yan-Dong , Zhang Yu-Yuan , Wang Haiwei , Cai Xuehui , Meng Fandan
Published: April 2022

Senecavirus A (SVA), an important member of the Picornaviridae family, causes vesicular disease in pigs. Here, we generated an EGFP-expressing recombinant SVA re-SVA-EGFP, which exhibited similar growth kinetics to its parental virus. The reporter SVA was used to study the role of pig ANTXR1 (pANTXR1) in SVA infection in a porcine alveolar macrophage cell line (PAM-Tang cells). Knockdown of the pANTXR1 significantly reduced SVA infection and replication in PAM-Tang cells, while re-expression of the pANTXR1 promoted the cell susceptibility to SVA infection. The results indicated that pANTXR1 is a crucial receptor mediating SVA infection. Subsequently, the viral endocytosis pathways for SVA entry into pig cells were investigated and the results showed that cholesterol played an essential role in receptor-mediated SVA entry. Together, these results demonstrated that SVA entered into host cells through the pANTXR1-mediated cholesterol pathway. Our findings provide potential targets to develop antiviral drugs for the prevention of SVA infection in the pig population.
Antimicrobial Peptides: Bringing Solution to the Rising Threats of Antimicrobial Resistance in Livestock
Authors: Saeed Shamsaldeen Ibrahim , Mergani AhmedElmontaser , Aklilu Erkihun , Kamaruzzaman Nor Fadhilah
Published: April 2022

Antimicrobial therapy is the most applied method for treating and preventing bacterial infection in livestock. However, it becomes less effective due to the development of antimicrobial resistance (AMR). Therefore, there is an urgent need to find new antimicrobials to reduce the rising rate of AMR. Recently, antimicrobial peptides (AMPs) have been receiving increasing attention due to their broad-spectrum antimicrobial activity, rapid killing activities, less toxicity, and cell selectivity. These features make them potent and potential alternative antimicrobials to be used in animals. Here, we discuss and summarize the AMPs in animals, classification, structures, mechanisms of action, and their potential use as novel therapeutic alternative antimicrobials to tackle the growing AMR threat.
First Identification and Genetic Characterization of a Novel Duck Astrovirus in Ducklings in China
Authors: Zhang Junqin , Huang Yunzhen , Li Linlin , Dong Jiawen , Kuang Ruihuan , Liao Ming , Sun Minhua
Published: April 2022

Four divergent groups of duck astroviruses (DAstVs) have been identified that infect domestic ducks. In March 2021, a fatal disease characterized by visceral urate deposition broke out in 5-day-old Beijing ducks on a commercial farm in Guangdong province, China. We identified a novel duck astrovirus from the ducklings suffering from gout disease. The complete genome sequence of this DAstV was obtained by virome sequencing and amplification. Phylogenetic analyses and pairwise comparisons demonstrated that this DAstV represented a novel group of avastrovirus. Thus, we designated this duck astrovirus as DAstV-5 JM strain. DAstV-5 JM shared genome sequence identities of 15–45% with other avastroviruses. Amino acid identities with proteins from other avastroviruses did not exceed 59% for ORF1a, 79% for ORF1b, and 60% for ORF2. The capsid region of JM shared genetic distances of 0.596 to 0.695 with the three official avastrovirus species. suggesting that JM could be classified as a novel genotype species in the Avastrovirus genus. Meanwhile, JM shares genetic distances of 0.402–0.662 with all the other known unassigned avastroviruses, revealing that it represents an additional unassigned avastrovirus. In summary, we determined that the DAstV-5 JM strain is a novel genotype species of avastrovirus.
Point Cloud Upsampling Algorithm: A Systematic Review
Authors: Zhang Yan , Zhao Wenhan , Sun Bo , Zhang Ying , Wen Wen
Published: April 2022

Point cloud upsampling algorithms can improve the resolution of point clouds and generate dense and uniform point clouds, and are an important image processing technology. Significant progress has been made in point cloud upsampling research in recent years. This paper provides a comprehensive survey of point cloud upsampling algorithms. We classify existing point cloud upsampling algorithms into optimization-based methods and deep learning-based methods, and analyze the advantages and limitations of different algorithms from a modular perspective. In addition, we cover some other important issues such as public datasets and performance evaluation metrics. Finally, we conclude this survey by highlighting several future research directions and open issues that should be further addressed.
Sensitivity of Four Indices of Meteorological Drought for Rainfed Maize Yield Prediction in the State of Sinaloa, Mexico
Authors: Omar Llanes-Cárdenas , Mariano Norzagaray-Campos , Alberto Gaxiola , Ernestina Pérez-González , Jorge Montiel-Montoya , Enrique Troyo-Diéguez
Published: April 2022

In the state of Sinaloa, rainfall presents considerable irregularities, and the climate is mainly semiarid, which highlights the importance of studying the sensitivity of various indices of meteorological drought. The goal is to evaluate the sensitivity of four indices of meteorological drought from five weather stations in Sinaloa for the prediction of rainfed maize yield. Using DrinC software and data from the period 1982–2013, the following were calculated: the standardized precipitation index (SPI), agricultural standardized precipitation index (aSPI), reconnaissance drought index (RDI) and effective reconnaissance drought index (eRDI). The observed rainfed maize yield (RMYob) was obtained online, through free access from the database of the Agrifood and Fisheries Information Service of the government of Mexico. Sensitivities between the drought indices and RMYob were estimated using Pearson and Spearman correlations. Predictive models of rainfed maize yield (RMYpr) were calculated using multiple linear and nonlinear regressions. In the models, aSPI and eRDI with reference periods and time steps of one month (January), two months (December–January) and three months (November–January), were the most sensitive. The correlation coefficients between RMYob and RMYpr ranged from 0.423 to 0.706, all being significantly different from zero. This study provides new models for the early calculation of RMYpr. Through appropriate planning of the planting–harvesting cycle of dryland maize, substantial socioeconomic damage can be avoided in one of the most important agricultural regions of Mexico.
Side Deep Fertilizing of Machine-Transplanted Rice to Guarantee Rice Yield in Conservation Tillage
Authors: Wu Qi-Xia , Du Bin , Jiang Shuo-Chen , Zhang Hai-Wei , Zhu Jian-Qiang
Published: April 2022

Conservation tillage is an environmentally friendly and economical farming method, but its impact on rice yield is controversial. Artificially applied side deep fertilizing of machine-transplanted rice is when fertilizer is applied to the deep soil along with the machine transplantation of rice; this may improve the fertilizer utilization rate and rice yield and eliminate the possible negative effects of conservation tillage on rice yield. Using on machine-transplanted rice, this study aims to compare the effects of side deep fertilizing (SDF). We investigated the effects of artificially applying fertilizer (AAF) on rice growth and yield under conventional tillage (CT), reduced tillage (RT), and no tillage (NT). The rice root activity, root dry weight, leaf area index (LAI), net photosynthetic rate (Pn), chlorophyll content, panicle density, spikelets per panicle, and yield were all ranked as NT > RT > CT and SDF > AAF. The 1000-grain weight was also ranked as SDF > AAF. In addition, under NT conditions, the positive effect of SDF on rice growth and yield was higher than under RT and CT conditions. In general, conservation tillage combined with SDF saved costs and increased rice yield.
Medicinal and Aromatic Plants in Agricultural Research, When Considering Criteria of Multifunctionality and Sustainability
Authors: Licata Mario , Maggio Antonella Maria , La Bella Salvatore , Tuttolomondo Teresa
Published: April 2022

Over the last twenty years, agriculture has witnessed significant changes regarding energy requirements, advanced technologies and practices [...]
Green Banana Maturity Classification and Quality Evaluation Using Hyperspectral Imaging
Authors: Chu Xuan , Miao Pu , Zhang Kun , Wei Hongyu , Fu Han , Liu Hongli , Jiang Hongzhe , Ma Zhiyu
Published: April 2022

Physiological maturity of bananas is of vital importance in determination of their quality and marketability. This study assessed, with the use of a Vis/NIR hyperspectral imaging (400–1000 nm), the feasibility in differentiating six maturity levels (maturity level 2, 4, and 6 to 9) of green dwarf banana and characterizing their quality changes during maturation. Spectra were extracted from three zones (pedicel, middle and apex zone) of each banana finger, respectively. Based on spectra of each zone, maturity identification models with high accuracy (all over 91.53% in validation set) were established by partial least squares discrimination analysis (PLSDA) method with raw spectra. A further generic PLSDA model with an accuracy of 94.35% for validation was created by the three zones’ spectra pooled to omit the effect of spectra acquisition position. Additionally, a spectral interval was selected to simplify the generic PLSDA model, and an interval PLSDA model was built with an accuracy of 85.31% in the validation set. For characterizing some main quality parameters (soluble solid content, SSC; total acid content, TA; chlorophyll content and total chromatism, ΔE*) of banana, full-spectra partial least squares (PLS) models and interval PLS models were, respectively, developed to correlate those parameters with spectral data. In full-spectra PLS models, high coefficients of determination (R2) were 0.74 for SSC, 0.68 for TA, and fair of 0.42 as well as 0.44 for chlorophyll and ΔE*. The performance of interval PLS models was slightly inferior to that of the full-spectra PLS models. Results suggested that models for SSC and TA had an acceptable predictive ability (R2 = 0.64 and 0.59); and models for chlorophyll and ΔE* (R2 = 0.34 and 0.30) could just be used for sample screening. Visualization maps of those quality parameters were also created by applying the interval PLS models on each pixel of the hyperspectral image, the distribution of quality parameters in which were basically consistent with the actual measurement. This study proved that the hyperspectral imaging is a useful tool to assess the maturity level and quality of dwarf bananas.
Soil Microbial Activity in Different Cropping Systems under Long-Term Crop Rotation
Authors: Kuht Jaan , Eremeev Viacheslav , Talgre Liina , Loit Evelin , Mäeorg Erkki , Margus Kalle , Runno-Paurson Eve , Madsen Helena , Luik Anne
Published: April 2022

Soil microbes play a key role in the nutrient cycling by decomposing the organic material into plant-available elements and also by maintaining the soil health. The study of soil microbial hydrolytic activity (SMA) was carried out in a long-term crop rotation (barley undersown (us) with red clover, red clover, winter wheat, pea and potato) experiment in five different farming systems during 2014–2018. There were two conventional systems, with chemical plant protection and mineral fertilizers, and three organic systems, which included winter cover crops and composted manure. The aim of the present study was to evaluate the effect of the (i) cropping system and (ii) precrops in rotation on the soil SMA. The soil microbial hydrolytic activity was significantly affected by yearly weather conditions, farming system, and crops. In all farming systems, the SMA was the lowest after dry and cold conditions during early spring in 2018. In unfertilized conventional systems, the considerably lower SMA is explained by the side effects of pesticides and low organic residuals, and we can conclude that the conventional system with no added fertilizer or organic matter is not sustainable, considering soil health. In each year, the SMA of organic systems with cover crops and composted manure was 7.3–14.0% higher compared to all farming systems. On average, for both farming systems, the SMA of all the rotation crops was positively correlated with the SMA values of precrops. However, in conventional farming systems, the effect of undersowing on the SMA of the precrop was smaller compared to organic systems.
Transforming the Farmer into a Rural Entrepreneur as a Path to Sustainability
Authors: Júnior Antonio Bliska , Bliska Flávia Maria de Mello
Published: April 2022

This work presents a tool to support the transformation of the farmer into a rural entrepreneur, raising the level of management of his company and increasing its competitiveness and sustainability in the long term, enabling its growth and not just its survival, but respecting social and environmental factors that permeate agricultural production. This tool, the Management Degree Identification Method—MIGG, will be able to support the elaboration of public policies for the agricultural segment and improve the service provided to rural entrepreneurs by rural extension and scientific and technological research areas. This tool emphasizes the creation and implementation of mechanisms for greater transparency, professionalization and sustainability in agriculture in different geographic regions.
Microbial Metabolomics Interaction and Ecological Challenges of Trichoderma Species as Biocontrol Inoculant in Crop Rhizosphere
Authors: Shahriar Saleh Ahmed , Islam M. Nazrul , Chun Charles Ng Wai , Kaur Parwinder , Rahim Md. Abdur , Islam Md. Mynul , Uddain Jasim , Siddiquee Shafiquzzaman
Published: April 2022

The fungal species belonging to the genus Trichoderma has been globally recognized as a potential candidate of biofertilizer and biocontrol agent to prevent devastating soil-borne fungal pathogens and enhance growth and productivity of agricultural crops. The antagonistic activity of Trichoderma to pathogenic fungi is attributed to several mechanisms including antibiosis and enzymatic hydrolysis, which are largely associated with a wide range of metabolites secreted by the Trichoderma species. Besides suppressing target pathogens, several metabolites produced by Trichoderma species may act against non-pathogenic beneficial soil microbial communities and perform unintended alterations within the structures and functions of microbial communities in the crop rhizosphere. Multiple microbial interactions have been shown to enhance biocontrol efficacy in many cases as compared to bioinoculant employed alone. The key advances in understanding the ecological functions of the Trichoderma species with special emphasis on their associations with plant roots and other microbes exist in the crop rhizosphere, which are briefly described here. This review focuses on the interactions of metabolites secreted by Trichoderma species and plant roots in the rhizosphere and their impacts on pathogenic and non-pathogenic soil microbial communities. The complex interactions among Trichoderma–plants–microbes that may occur in the crop rhizosphere are underlined and several prospective avenues for future research in this area are briefly explored. The data presented here will stipulate future research on sustainably maximizing the efficiency of Trichoderma inoculation and their secondary metabolites in the crop soil ecosystem.
Differential and Interactive Effects of Scleroderma sp. and Inorganic Phosphate on Nutrient Uptake and Seedling Quality of Castanea henryi
Authors: Zuo Ronghua , Zou Feng , Tian Shiyi , Masabni Joseph , Yuan Deyi , Xiong Huan
Published: April 2022

Both ectomycorrhizal fungi (ECMF) and inorganic phosphate (Pi) can improve plant growth. However, the relationship between Pi levels and mycorrhizal colonization rate is divergent, and information on the differential and interactive effects of Pi levels and ECMF on nutrient uptake and seedling quality is lacking. We conducted a study on 4-week-old Castanea henryi ‘You Zhen’ seedlings by inoculating with Scleroderma sp. (+ECM) to compare with uninoculated (-ECM), under low Pi level (-Pi) and high Pi level (+Pi). The mycorrhizal colonization rate, seedlings morphological and physiological parameters, nutrient uptake content, and the rhizosphere soil enzymatic activities and chemical properties were investigated. Results indicated that the mycorrhizal colonization rate at low Pi level (84 ± 2%) was higher than that at high Pi level (71 ± 2.52%). No matter the Pi level, inoculating with ECMF significantly improved seedling dry weight, height, diameter, and seedling quality index (SQI). The +ECM+Pi and +ECM-Pi treatments significantly increased SQI by 282.76% and 232.76% in comparison to the -ECM-Pi treatment, respectively. Applied Pi had no significant effect on SQI. ECMF inoculation significantly increased nitrogen (N), phosphorus (P), and potassium (K) uptake in roots, stems, and leaves, while Pi application increased the P uptake in roots only. There was no significant interaction between ECMF and Pi levels on seedling quality and nutrient uptake (except P uptake in leaf). Results indicated that ECMF is a suitable alternative to the use of phosphorus fertilizer in nursery production of C. henryi seedlings in terms of protecting the environment, saving resources, and reducing production costs.
Virus Infection in Equine
Authors: Steinman Amir , Erster Oran , Tirosh-Levy Sharon
Published: April 2022

The relationship between men and horses has significantly evolved over the last century [...]
Effects of Pathogenic Escherichia coli Infection on the Flora Composition, Function, and Content of Short-Chain Fatty Acids in Calf Feces
Authors: He Lina , Wang Chunjie , Simujide Huasai , Aricha Han , Zhang Jian , Liu Bo , Aorigele Chen
Published: April 2022

Calf diarrhea caused by pathogenic Escherichia coli is a major cause of death in calves, with a mortality rate of over 50%. It is crucial to understand the pathogenesis and development of calf diarrhea for its prevention and treatment. We aimed to study the effect of pathogenic E. coli on the flora composition, function, and short-chain fatty acid (SCFA) content of calf feces using a calf diarrhea model. Sixty-four newborn Holstein calves (40–43 kg) were divided into a normal group (NG; n = 32) and a test group (TG; n = 32). At the beginning of the experiment, the TG were orally administered pathogenic E. coli O1 (2.5 × 1011 CFU/mL, 100 mL) to establish a calf diarrhea model, and the NG were orally administered the same amount of physiological saline solution. The calves of the two groups were subjected to the same feeding and management. Fresh feces samples were collected at different time points and subjected to 16S rRNA high-throughput sequencing and gas chromatography–mass spectrometry to determine the fecal microbial composition and SCFA content. Pathogenic E. coli O1 significantly altered microbiotas composition in the feces of calves, increasing the relative abundance of Proteobacteria and decreasing that of Firmicutes. It also led to a significant increase in the relative abundance of Escherichia-Shigella and a decrease in Lactobacillus, as well as significantly decreased SCFA content. Therefore, we postulate that pathogenic E. coli induces calf diarrhea by causing intestinal florae imbalance and reducing the content of SCFA.
High Prevalence of Tick-Borne Zoonotic Rickettsia slovaca in Ticks from Wild Boars, Northeastern Italy
Authors: Grassi Laura , Menandro Maria Luisa , Cassini Rudi , Mondin Alessandra , Pasotto Daniela , Grillini Marika , Rocca Giuseppe , Drigo Michele
Published: April 2022

Tick-borne rickettsiae are emerging pathogens that are becoming widespread in Europe. Rickettsiae are endemic in Italy, but epidemiological data are currently scarce. This study aimed to improve our knowledge about rickettsial infections in tick and wild boar populations. Blood and ticks were collected from 102 wild boars in 2010 and 2018. Ticks were also collected from the vegetation in the area. All of the samples were examined using real-time PCR targeting the gltA gene to detect Rickettsia DNA. Positivity was confirmed by PCR amplifying the gltA and/or ompB genes. A total of 254 ticks and 89 blood samples were analyzed. Zoonotic rickettsiae were detected in the ticks but not in the blood samples. Rickettsia slovaca (R. slovaca) was the most prevalent in ticks and was found in 23.7% of Dermacentor marginatus (D. marginatus) and in 3.4% of Ixodes ricinus (I. ricinus). Other zoonotic species were identified, such as Rickettsia monacensis, which was detected in 12% of I. ricinus ticks, and Rickettsia helvetica which was found in 3.4% of questing I. ricinus ticks and in 1.1% of D. marginatus collected from wild boars. This study highlights a high prevalence of zoonotic rickettsiae, particularly that of R. slovaca, in northeastern Italy. As rickettsioses are underreported and underdiagnosed in human medicine, both clinicians and researchers should pay more attention to this topic.
Retrotransposon Insertion Polymorphisms (RIPs) in Pig Coat Color Candidate Genes
Authors: Du Zhanyu , D’Alessandro Enrico , Zheng Yao , Wang Mengli , Chen Cai , Wang Xiaoyan , Song Chengyi
Published: April 2022

The diversity of livestock coat color results from human positive selection and represents an indispensable part of breed identity. As an important biodiversity resource, pigs have many special characteristics, including the most visualized feature, coat color, and excellent adaptation, and the coat color represents an important phenotypic characteristic of the pig breed. Exploring the genetic mechanisms of phenotypic characteristics and the melanocortin system is of considerable interest in domestic animals because their energy metabolism and pigmentation have been under strong selection. In this study, 20 genes related to coat color in mammals were selected, and the structural variations (SVs) in these genic regions were identified by sequence alignment across 17 assembled pig genomes, from representing different types of pigs (miniature, lean, and fat type). A total of 167 large structural variations (>50 bp) of coat-color genes, which overlap with retrotransposon insertions (>50 bp), were obtained and designated as putative RIPs. Finally, 42 RIPs were confirmed by PCR detection. Additionally, eleven RIP sites were further evaluated for their genotypic distributions by PCR in more individuals of eleven domesticated breeds representing different coat color groups. Differential distributions of these RIPs were observed across populations, and some RIPs may be associated with breed differences.
Combating Bacterial Biofilm Formation in Urinary Catheter by Green Silver Nanoparticle
Authors: Goda Reham M. , El-Baz Ahmed M. , Khalaf Eman M. , Alharbi Nada K. , Elkhooly Tarek A. , Shohayeb Mohamed M.
Published: April 2022

Urinary catheters are commonly associated with urinary tract infections. This study aims to inhibit bacterial colonisation and biofilm of urinary tract catheters. Silicon catheter pieces were varnished with green silver nanoparticles (AgNPs) using Pistacia lentiscus mastic to prevent bacterial colonisation. Pomegranate rind extract was used to synthesize AgNPs. AgNPs were characterized by UV-Vis spectroscopy, X-ray crystallography, and transmission electron microscopy (TEM). Results obtained revealed that the size of most AgNPs ranged between 15–25 nm and they took crystallised metal and oxidised forms. The amounts of released silver ions from 1 cm pieces of catheters coated with AgNPs were estimated for five days and ranged between 10.82 and 4.8 µg. AgNPs coated catheters significantly inhibited the colonisation of catheters by antibiotic-resistant clinical Gram-positive (Staphylococcus epidermidis and Staphylococcus aureus) and Gram-negative (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa) bacteria. AgNPs-varnish was more active against Gram-negative bacteria than Gram-positive bacteria. The significant inhibitory effect of coated catheters lasted for 72 h for both Gram-positive and Gram-negative bacteria. Varnishing catheters with AgNPs may help to prevent bacterial colonisation and infections.
Phenolic Compound Ethyl 3,4-Dihydroxybenzoate Retards Drug Efflux and Potentiates Antibiotic Activity
Authors: Lu Wen-Jung , Huang Yan-Jyun , Lin Hsuan-Ju , Chang Chun-Ju , Hsu Pang-Hung , Ooi Gui-Xia , Huang Mei-Ying , Lin Hong-Ting Victor
Published: April 2022

The World Health Organization indicated that antibiotic resistance is one of the greatest threats to health, food security, and development in the world. Drug resistance efflux pumps are essential for antibiotic resistance in bacteria. Here, we evaluated the plant phenolic compound ethyl 3,4-dihydroxybenzoate (EDHB) for its efflux pump inhibitory (EPI) activity against drug-resistant Escherichia coli. The half-maximal inhibitory concentration, modulation assays, and time-kill studies indicated that EDHB has limited antibacterial activity but can potentiate the activity of antibiotics for drug-resistant E. coli. Dye accumulation/efflux and MALDI-TOF studies showed that EDHB not only significantly increases dye accumulation and reduces dye efflux but also increases the extracellular amount of antibiotics in the drug-resistant E. coli, indicating its interference with substrate translocation via a bacterial efflux pump. Molecular docking analysis using AutoDock Vina indicated that EDHB putatively posed within the distal binding pocket of AcrB and in close interaction with the residues by H-bonds and hydrophobic contacts. Additionally, EDHB showed an elevated postantibiotic effect on drug-resistant E. coli. Our toxicity assays showed that EDHB did not change the bacterial membrane permeability and exhibited mild human cell toxicity. In summary, these findings indicate that EDHB could serve as a potential EPI for drug-resistant E. coli.
Alternative Pathways to Ciprofloxacin Resistance in Neisseria gonorrhoeae: An In Vitro Study of the WHO-P and WHO-F Reference Strains
Authors: González Natalia , Abdellati Saïd , De Baetselier Irith , Laumen Jolein Gyonne Elise , Van Dijck Christophe , de Block Tessa , Kenyon Chris , Manoharan-Basil Sheeba Santhini
Published: April 2022

Emerging resistance to ceftriaxone and azithromycin has led to renewed interest in using ciprofloxacin to treat Neisseria gonorrhoeae. This could lead to the rapid emergence and spread of ciprofloxacin resistance. Previous studies investigating the emergence of fluoroquinolone resistance have been limited to a single strain of N. gonorrhoeae. It is unknown if different genetic backgrounds affect the evolution of fluoroquinolone resistance in N. gonorrhoeae, as has been shown in other bacterial species. This study evaluated the molecular pathways leading to ciprofloxacin resistance in two reference strains of N.gonorrhoeae—WHO-F and WHO-P. Three clones of each of the two strains of N.gonorrhoeae were evolved in the presence of ciprofloxacin, and isolates from different time points were whole-genome sequenced. We found evidence of strain-specific differences in the emergence of ciprofloxacin resistance. Two out of three clones from WHO-P followed the canonical pathway to resistance proceeding via substitutions in GyrA-S91F, GyrA-D95N and ParC. None of the three WHO-F clones followed this pathway. In addition, mutations in gyrB, uvrA and rne frequently occurred in WHO-F clones, whereas mutations in yhgF, porB and potA occurred in WHO-P.
Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress
Authors: Skibska Beata , Goraca Anna , Skibska Agnieszka , Stanczak Andrzej
Published: April 2022

Alpha-lipoic acid (α-LA) is a disulfide compound and one of the most effective antioxidants. Many studies have indicated positive effects of α-LA in the prevention of pathologic conditions mediated by oxidative stress, such as cardiovascular diseases. However, the therapeutic potential of α-LA for the heart has not been explored with regards to the ventricles and atria. The aim of our study was to evaluate the effects of α-LA on oxidative stress parameters and inflammation in the ventricles and atria of the heart in rats under LPS-induced oxidative stress. Wistar rats were divided into 4 groups: I—control (received 2 doses of 0.2 mL of 0.9% NaCl i.v., 0.5 h apart); II—α-LA (received 0.2 mL of 0.9% NaCl and 0.5 h later received α-LA 60 mg/kg b.w. i.v.); III—lipopolysaccharide (LPS) (received 0.2 mL of 0.9% NaCl and 0.5 h later received LPS 30 mg/kg b.w. i.v.); and IV—LPS + LA (received LPS 30 mg/kg b.w. i.v. and 0.5 h later received α-LA 60 mg/kg b.w. i.v.). Five hours later, the rats were euthanized. The hearts were surgically removed and weighed to estimate heart edema. The ventricular and atrium tissue was isolated to measure levels of TNF-α, IL-6, superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), total sulfhydryl groups (-SH), total glutathione (tGSH), reduced glutathione (GSH), glutathione disulfide (GSSG), and the GSH/GSSG ratio. LPS significantly increased TNF-α, IL-6, TBARS, and H2O2 levels and decreased SOD, -SH groups, tGSH, the GSH/GSSG ratio, and GSH levels in rat ventricles and atria while α-LA administered after the injection of LPS significantly decreased TNF-α, IL-6, TBARS, and H2O2 levels. α-LA also increased SOD and -SH group levels and ameliorated the glutathione redox status when compared to the LPS group. Our data suggest that α-LA administration 30 min after LPS infusion may effectively prevent inflammation and oxidative stress in the ventricles and atria.
Identification and Characterization of Two Bibenzyl Glycosyltransferases from the Liverwort Marchantia polymorpha
Authors: Xiong Rui-Lin , Zhang Jiao-Zhen , Liu Xin-Yan , Deng Jian-Qun , Zhu Ting-Ting , Ni Rong , Tan Hui , Sheng Ju-Zheng , Lou Hong-Xiang , Cheng Ai-Xia
Published: April 2022

Liverworts are rich in bibenzyls and related O-glycosides, which show antioxidant activity. However, glycosyltransferases that catalyze the glycosylation of bibenzyls have not yet been characterized. Here, we identified two bibenzyl UDP-glucosyltransferases named MpUGT737B1 and MpUGT741A1 from the model liverwort Marchantia polymorpha. The in vitro enzymatic assay revealed that MpUGT741A1 specifically accepted the bibenzyl lunularin as substrate. MpUGT737B1 could accept bibenzyls, dihydrochalcone and phenylpropanoids as substrates, and could convert phloretin to phloretin-4-O-glucoside and phloridzin, which showed inhibitory activity against tyrosinase and antioxidant activity. The results of sugar donor selectivity showed that MpUGT737B1 and MpUGT741A1 could only accept UDP-glucose as a substrate. The expression levels of these MpUGTs were considerably increased after UV irradiation, which generally caused oxidative damage. This result indicates that MpUGT737B1 and MpUGT741A1 may play a role in plant stress adaption. Subcellular localization indicates that MpUGT737B1 and MpUGT741A1 were expressed in the cytoplasm and nucleus. These enzymes should provide candidate genes for the synthesis of bioactive bibenzyl O-glucosides and the improvement of plant antioxidant capacity.
Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts
Authors: Lin His , Cheng Kuan-Chen , Lin Jer-An , Hsieh Liang-Po , Chou Chun-Hsu , Wang Yu-Ying , Lai Ping-Shan , Chu Po-Cheng , Hsieh Chang-Wei
Published: April 2022

Ultraviolet-A (UVA) exposure is a major cause of skin aging and can induce oxidative damage and accelerate skin wrinkling. Many natural polysaccharides exhibit a UV protective effect. In research on Pholiota nameko polysaccharides (PNPs), a natural macromolecular polysaccharide (4.4–333.487 kDa), studies have shown that PNPs can significantly decrease elastase activity to protect against UVA-induced aging in Hs68 human dermal fibroblasts. Cellular experiments in the present study indicated that PNPs can protect against UVA-induced oxidative damage in Hs68 cells by inhibiting the production of reactive oxygen species. Furthermore, PNPs significantly attenuated UVA-induced cell aging by decreasing the protein expression of matrix metalloproteinase 1, 3, and 9. Pretreatment of Hs68 cells with PNP-40, PNP-60, and PNP-80 before UVA irradiation increased protein expression of tissue inhibitor metalloproteinase 1 by 41%, 42%, and 56% relative to untreated cells. In conclusion, this study demonstrates that PNPs are a natural resource with potentially beneficial effects in protecting against UVA-induced skin aging.
Multicentric Standardization of Protocols for the Diagnosis of Human Mitochondrial Respiratory Chain Defects
Authors: Bujan Nuria , Morén Constanza , García-García Francesc J. , Blázquez Alberto , Carnicer Clara , Cortés Ana Belén , González Cristina , López-Gallardo Ester , Lozano Ester , Moliner Sonia , Gort Laura , Tobías Ester , Delmiro Aitor , Martin Miguel Ángel , Fernández-Moreno Miguel Ángel , Ruiz-Pesini Eduardo , Garcia-Arumí Elena , Rodríguez-Aguilera Juan Carlos , Garrabou Glòria
Published: April 2022

The quantification of mitochondrial respiratory chain (MRC) enzymatic activities is essential for diagnosis of a wide range of mitochondrial diseases, ranging from inherited defects to secondary dysfunctions. MRC lesion is frequently linked to extended cell damage through the generation of proton leak or oxidative stress, threatening organ viability and patient health. However, the intrinsic challenge of a methodological setup and the high variability in measuring MRC enzymatic activities represents a major obstacle for comparative analysis amongst institutions. To improve experimental and statistical robustness, seven Spanish centers with extensive experience in mitochondrial research and diagnosis joined to standardize common protocols for spectrophotometric MRC enzymatic measurements using minimum amounts of sample. Herein, we present the detailed protocols, reference ranges, tips and troubleshooting methods for experimental and analytical setups in different sample preparations and tissues that will allow an international standardization of common protocols for the diagnosis of MRC defects. Methodological standardization is a crucial step to obtain comparable reference ranges and international standards for laboratory assays to set the path for further diagnosis and research in the field of mitochondrial diseases.
Biosynthesis of (±)-Differolide, an Antioxidant Isolate from Streptomyces qaidamensis S10T
Authors: Wu Yujie , Zhang Wei , Jiang Kan , Yu Xue , Wu Shiyu , Liu Guangxiu , Chen Tuo
Published: April 2022

Streptomyces from unexplored or underexplored environments may be an essential source of discoveries of bioactive molecules. One such example is Streptomyces qaidamensis S10T, which was isolated from a sand sample collected in Qaidam Basin, Qinghai Province, China. Here, we report on (±)-differolide, an antioxidant isolated from S. qaidamensis, and verified with scavenging experiments on 2,2-diphenyl-1-picrylhydrazyl (DPPH). The biosynthetic gene cluster responsible for synthesizing the compound was also identified using comparative genomic methods. These results provide a basis for further study of the biological activities of (±)-differolide, which also make it possible to develop as an antioxidant medicine.
Experimental and Simulation Research on the Process of Nitrogen Migration and Transformation in the Fluctuation Zone of Groundwater Level
Authors: Li Yuepeng , Wang Liuyue , Zou Xun , Qu Jihong , Bai Gang
Published: April 2022

The fluctuation of groundwater causes a change in the groundwater environment and then affects the migration and transformation of pollutants. To study the influence of water level fluctuations on nitrogen migration and transformation, physical experiments on the nitrogen migration and transformation process in the groundwater level fluctuation zone were carried out. A numerical model of nitrogen migration in the Vadose zone and the saturated zone was constructed by using the software HydrUS-1D. The correlation coefficient and the root mean square error of the model show that the model fits well. The numerical model is used to predict nitrogen migration and transformation in different water level fluctuation scenarios. The results show that, compared with the fluctuating physical experiment scenario, when the fluctuation range of the water level increases by 5 cm, the fluctuation range of the nitrogen concentration in the coarse sand, medium sand and fine sand media increases by 37.52%, 31.40% and 21.14%, respectively. Additionally, when the fluctuation range of the water level decreases by 5 cm, the fluctuation range of the nitrogen concentration in the coarse sand, medium sand and fine sand media decreases by 36.74%, 14.70% and 9.39%, respectively. The fluctuation of nitrogen concentration varies most significantly with the amplitude of water level fluctuations in coarse sand; the change in water level has the most significant impact on the flux of nitrate nitrogen and has little effect on the change in nitrite nitrogen and ammonium nitrogen, and the difference in fine sand is the most obvious, followed by medium sand, and the difference in coarse sand is not great.
THz Time-Domain Ellipsometer for Material Characterization and Paint Quality Control with More Than 5 THz Bandwidth
Authors: Ketelsen Helge , Mästle Rüdiger , Liebermeister Lars , Kohlhaas Robert , Globisch Björn
Published: April 2022

Quality control of car body paint in the automotive industry is a promising industrial application of terahertz technology. Terahertz time-domain spectroscopy in reflection geometry enables accurate, fast, and nondestructive measurement of individual layer thicknesses of multi-layer coatings. For high precision thickness measurements, the frequency dependent complex refractive index of all layers must be calibrated very accurately. THz time-domain ellipsometry is self-referencing and provides reliable, frequency resolved material properties with high signal-to-noise ratio. The method is characterized by a high sensitivity to optical material properties and layer thicknesses. We present characterization results in the frequency range 0.1–6 THz for typical automotive paints and different substrates such as polypropylene (PP), which features a high material anisotropy. We demonstrate that the broadband material properties derived from ellipsometry allow for inline thickness measurements of multi-layer car body paints with high accuracy.
Uni-Planar MIMO Antenna for Sub-6 GHz 5G Mobile Phone Applications
Authors: Rafique Umair , Khan Suleman , Ahmed Muhammad Mansoor , Kiani Saad Hassan , Abbas Syed Muzahir , Saeed Sohail Imran , Alibakhshikenari Mohammad , Dalarsson Mariana
Published: April 2022

This article presents the design of a uni-planar MIMO antenna system for sub-6 GHz 5G-enabled smartphones. The MIMO antenna designed comprises four loop-shaped radiators placed at each corner of the mobile phone board, which follows the principle of pattern diversity. The single-antenna element resonates at 3.5 GHz, its impedance bandwidth is noted to be 1.28 GHz (3–4.28 GHz) for S11≤ −6 dB, and it is equal to 720 MHz (3.18–3.9 GHz) for S11≤ −10 dB. For a single-antenna element, a peak gain of 3.64 dBi is observed with an antenna efficiency of >90%. The isolation of >10 dB between antenna elements is achieved for the MIMO configuration. Furthermore, the MIMO antenna designed provides enough radiation coverage to support different sides of the mobile phone board, which is an important feature for future 5G-enabled handsets. In addition, the impacts of human hands and heads on MIMO antenna performance are investigated, and acceptable performance in the data and conversation modes is observed.
Natural Salicylaldehyde for Fungal and Pre- and Post-Emergent Weed Control
Authors: Kim Jong H. , Chan Kathleen L.
Published: April 2022

A sustainable, alternative weed control strategy is developed using salicylaldehyde (SA; 2-hydroxybenzaldehyde) as an active ingredient. SA is a natural, redox-active small molecule listed as a Generally Recognized As Safe food additive by the European Food Safety Authority and the United States Food and Drug Administration. The repurposing of SA determined that SA possesses both pre- and post-emergent herbicidal, fumigant activity, where the emitted SA from the source completely prevented the germination of plant seeds and/or the growth of the germinated plants. As a proof-of-concept, we developed agricultural byproducts (tree nutshell particles) as SA delivery vehicles to the soil, thus helping the growers’ sustainable byproduct recycling program, necessary for carbon sequestration. In plate assays, SA emitted from the nutshell vehicles (0.15 to 1.6 M) completely prevented the germination of six invasive or native weed seeds (monocots, dicots). In Magenta vessel assays, SA emitted from the nutshell vehicles (0.8 to 1.6 M) not only prevented the germination (pre-emergent) of Lagurus ovatus (Bunny Tails Grass) seeds but also inhibited the growth (post-emergent) of the germinated weeds. We determined further that soil covering (soil pasteurization) could be one of the practices to effectively deliver SA to the soil, whereby 1.6 M of SA emitted from the nutshell vehicles prevented the germination of the L. ovatus seeds maintained in soil trays covered with plastic tarp at 22 °C, while 0.8 M SA allowed partial (15%) germination of the weed seeds. Of note, SA also possesses an intrinsic antifungal activity that overcomes the tolerance of the stress signaling mutants of filamentous fungal pathogens (Aspergillus fumigatus, Penicillium expansum) to the phenylpyrrole fungicide fludioxonil. Environmental degradation data available in the public database indicate that, once released to the environment, SA will be broken down in the air by sunlight or microorganisms and, thus, is not built up in aquatic organisms. Altogether, SA can serve as a safe, potent pesticide (herbicidal, fungicidal) ingredient that promotes sustainable crop production by lowering the pesticide burden in fields.
A New Vehicle Dataset in the City of Los Angeles for V2X and Machine Learning Applications
Authors: Alablani Ibtihal Ahmed , Arafah Mohammed Amer
Published: April 2022

The fifth-generation (5G) network is the current emerging technology that meets the increasing need for higher throughputs and greater system capacities. It is expected that 5G technology will enable many new applications and services. Vehicle-to-everything (V2X) communication is an example of an application that is supported by 5G technology and beyond. A V2X communication system allows a vehicle to be connected to an entity, such as a pedestrian, another vehicle, infrastructure, and a network, to provide a robust transportation solution. It uses many models and strategies that are usually based on machine learning (ML) techniques, which require the use of a vehicle dataset. In this paper, a real vehicle dataset is proposed that was generated in the city of Los Angeles (LA). It is called the Vehicle dataset in the city of LA (VehDS-LA). It has 74,170 samples that are located on 15 LA streets and each sample has 4 features. The LA dataset has been opened to allow researchers in V2X and ML fields to use it for academic purposes. The main uses of the VehDS-LA dataset are studies related to 5G networks, vehicle automation, or ML-Based vehicle mobility applications. The proposed dataset overcomes limitations experienced by previous related works.