bluuebunny/crossref_metadata_2025 huggingface dataset:
Digestive system and growth performance of African catfish larvae Clarias gariepinus, (Burchell, 1822) maintained with biofloc technology with the addition of Chlorella sp.
Authors: Nugraha Sujaka , Ekasari Julie , Junior M Zairin , Widanarni Widanarni
Published: December 2020

The production of catfish in aquaculture is still limited by the low supply of good quality seeds. One solution that can be done to overcome this problem is by the application of biofloc technology with microalgae addition. This study aims to evaluate the performance of digestive system, growth and robustness of the African catfish larvae maintained with biofloc technology and the addition of Chlorella sp. This research applied a completely randomized experimental design consisted of three treatments and triplicates, i.e larvae maintained with regular water exchange as the control (K), larvae maintained biofloc system (BF) and larvae reared with biofloc treatment and Chlorella sp. addition (BFC) with a rearing period of 15 days. Length growth, specific growth rate, condition factor, the activity of protease, amylase and lipase were not significantly different between treatments (P>0.05). The villi length in fish maintained in BF treatmen (136μm), was higher than those of BFC (121μm) and K treatments (105μm). The particle size of floc in BF and BFC were 0.44±0.025 and BFC 0.79±0.048 mm, respectively. The survival of catfish larvae in the BFC treatment was (51 ± 0,32)b, which was significantly higher (P<0.05) than those of K (45±0.52)a and BF (45±0.15)a. The results of stress test using 15 g L-1 water salinity demonstrated that the fish maintained in BFC has a higher survival (63%) than those of BF (47%) and K (43%). Overall results of the present study showed that the addition of Chlorella sp. could improve the growth performance and robustness of African catfish larvae against salinity stress.
PENGARUH KUALITAS LAYANAN, KUALITAS PRODUK, DAN HARGA TERHADAP KEPUASAN NASABAH KREDIT SERBA BISA PADA PD. BPR BKK KOTA SEMARANG PERIODE JUNI 2019
Authors: Ardani Ika Puspita , Handayani Jati
Published: December 2020

This purpose of this research is to analyze the effect of service quality, product quality, and price on customer satisfaction of Credit Serba Bisa at PD. BPR BKK Kota Semarang Period June 2019. This research is a causal applied quantitative research. The types of data used in this study are primary and secondary. Methods of data collection using questionnaires, interviews, and observation. The sampling technique used was purposive sampling technique with a sample of 100 respondents. Data testing methods used in this study are validity test, reliability test, normality test, multicollinearity test, heteroscedasticity test, autocorrelation test, multiple linear regression test, F test, t test, test the coefficient of determination R2 which shows can be used to measure customer satisfaction. The analytical method used in this study is multiple linear regression using SPSS 25. The results of the F test show that the quality of service, product quality, and price simultaneously have a significant effect on customer satisfaction on Credit Serba Bisa in PD. BPR BKK Kota Semarang. Furthermore, the t test results showed that service quality, product quality, and price partially had a significant effect on customer satisfaction of Credit Serba Bisa at PD. BPR BKK Kota Semarang.
ANALISIS KEPUASAN NASABAH ATAS KUALITAS LAYANAN INTERNET BANKING PADA PT BANK PEMBANGUNAN DAERAH JAWA TENGAH KANTOR CABANG PATI
Authors: Mutiasari Anita Elvi , Soemarso Embun Duriany
Published: December 2020

This research aims to identify how to increase customer satisfaction of internet banking service quality at PT Bank Pembangunan Daerah Jawa Tengah Pati Branch Office with five dimensions of service quality which were tangible, reliability, responsiveness, assurance, and empathy.

In this research, the sampling technique used was purposive sampling technique. The data in this research were obtained from observations and distributing questionnaires to 100 respondents. Data analysis methods used are validity test, reliability test, satisfaction index test, satisfaction gap test and satisfaction mapping with cartesian diagram.
Based on the result of the validity and reliability test, the indicators like tangible, reliability, responsiveness, assurance and empathy are valid and reliable. Based on the result of the satisfaction index analysis, the weighting score of internet banking quality service is 4,219. That score is between 3,43 – 4,23 wich means Satisfied. Based on the result of the satisfaction gap test the increase of satisfaction established on the fourteen sub-indicators show satisfaction, and four sub-indicators show dissatisfaction. Meanwhile, when viewed from the cartesian diagram mapping result, there are four indicators that fall into the quadrant I, four indicators in the quadrant II, four indicators in the quadrant III, and six indicators in the quadrant IV.
THE ANALYSIS OF THE EFFECT OF BRAND IMAGE, SERVICE PERFORMANCE, PORTFOLIO SERVICES, AND PHYSICAL EVIDENCES TOWARDS CUSTOMERS SATISFACTION OF “BTN BATARA” SAVINGS ACCOUNT AT PT BANK TABUNGAN NEGARA (PERSERO) Tbk, SEMARANG BRANCH OFFICE
Authors: Rofi’ah Nailur , Kodir Moch. Abdul , Muslikh Moch.
Published: December 2020

The objectives of this Final Report are to analyze and to obtain empirical evidences on the effect of brand image, service performance, portfolio services, and physical evidences towards customers satisfaction of “BTN Batara” Savings Account at PT Bank Tabungan Negara (Persero) Tbk, Semarang Branch Office.

The population of this Final Report is all of the customers at PT Bank Tabungan Negara (Persero) Tbk, Semarang Branch Office. The sampling technique uses the Random Sampling Method amounting to 100 customers. The data used in this Final Report is the primary data obtained throough the distribution of questionnaires. The data analysis model uses the Multiple Linear Regression Analysis Model. Meanwhile, data analysis techniques use F Test, Coefficient of Determination (R2), and t Test.
The Results of the analysis and discussion show that the variables of brand image, service performance, portfolio services, and physical evidences simultaneously have a significant effect towards customers satisfaction at PT Bank Tabungan Negara (Persero) Tbk, Semarang Branch Office. Partially, variable of brand image and physical evidences each have positive and significant effects towards customers satisfaction at PT Bank Tabungan Negara (Persero) Tbk, Semarang Branch Office. Meanwhile, variable of service performance does not have positive effect nor it is not significant. While portfolio services have positive effect but it is not significant towards customers satisfaction at PT Bank Tabungan Negara (Persero) Tbk, Semarang Branch Office.
Comparing the Musculoskeletal Injuries Between the Professional Greco-Roman and Freestyle Wrestlers
Authors:   Department of Corrective Exercises and Sports Injuries, Faculty of Physical Education and Sports Sciences, University of Guilan, Rasht, Iran., Daneshmandi Hasan , Zolghadr Hamid ,   https://orcid.org/0000-0002-8856-9810, Sedaghati Parisa ,   Department of Corrective Exercises and Sports Injuries, Faculty of Physical Education and Sports Sciences, University of Guilan, Rasht, Iran.
Published: December 2020

Purpose: Wrestling, due to its contact and oppressive training nature, is subject to massive injuries. The present study aimed to compare musculoskeletal injuries between professional Greco-Roman and freestyle wrestlers. Methods: This was a retrospective cross-sectional study. The statistical population consists of male Greco-Roman and freestyle wrestlers (N=86; age: 15-20 years) in Guilan Province, Iran. The study participants had a history of at least two continual years of wrestling training of 3 weekly sessions and participating in national and international competitions. The modified Fuller and Hawkins questionnaire was applied for recording recent two-year injuries. Using the descriptive statistics, the retrieved data were summarized and classified in tables. Besides, the difference between the study variables was determined by the Chi-squared test in SPSS. Results: A statistically significant difference was observed in neck injuries between the investigated Greco-Roman and freestyle wrestlers (P=0.01). Additionally, a significant difference was observed in the frequency of training per week (P=0.01), techniques (P=0.001), and injury mechanisms (P=0.02) between the two study groups. Conclusion: The present study findings revealed a significant difference in neck injury between Greco-Roman and freestyle wrestlers. Furthermore, a significant difference was detected in the frequency of training, techniques, mechanism of injury, and the injury rate between the study groups. Designing appropriate training sessions and plans and teaching the principles of warm-up and procedures for preventing additional damage in wrestlers are suggested to coaches
Comparing the Kinematics of the Lower Limb Joints in Jump-Landing Maneuver Among Soccer Players With and Without Histories of Anterior Cruciate Ligament Reconstruction
Authors:   Department of Corrective Exercise And Sport Injury, Faculty of Physical Education and Sport Sciences, Allemeh Tabataba'i University, Tehran, Iran., Ghorbani Moosareza , Babakhani Farideh ,   Department of Corrective Exercise And Sport Injury, Faculty of Physical Education and Sport Sciences, Allemeh Tabataba'i University, Tehran, Iran.
Published: December 2020

Background: Jumping and landing are common activities in soccer, i.e., associated with Anterior Cruciate Ligament (ACL) injuries in the players, especially when experiencing fatigue. The current study aimed to compare the kinematics of the lower limb joints in jump-landing maneuver among soccer players with and without a history of anterior cruciate ligament reconstruction. Methods: Among active soccer players in the provincial league level in Hamedan Province, Iran, 12 individuals with the intact knee (control group), and 12 individuals with 6 to 24 months post anterior cruciate ligament reconstruction (experimental group) were selected by convivence sampling method as the study participants. During the jump-landing task, the range of motion and the joints' angle at the contact instance were measured before and after performing plyometric fatigue protocol using the Vicon motion capture system. Multivariate Analysis of Variance (MANOVA) at a significance level of P<0.05 was used for intergroup comparisons. Results: Plyometric fatigue did not affect most of the kinematic variables before and after fatigue experience. Besides, only the hip range of motion revealed a significant difference between the study groups (P=0.38). Conclusion: Fatigue had more effect on some variables in individuals with ACL reconstruction, including the left and right hips angles. Moreover, there was a significant difference in some variables between the study groups; the range of motion of the left hip, the contact angle of the right hip before fatigue experience, and the contact angle of the right and left hips after fatigue. In general, a proper rehabilitation program is recommended for athletes with ACL reconstruction to reduce the risk of recurrence of jumping movements in exercise.
Mortalidade precoce das empresas do ramo de pet shop
Authors: Ferreira Roberto Soares Martins , Rocha Arnaldo
Published: December 2020

O Brasil, quarto país em população animal do mundo, é também o segundo colocado mundial no mercado pet, perdendo somente para os Estados Unidos. Cerca de 90% dos lares brasileiros possuem algum animal de estimação, como cão e gato, sendo que do total de 8000 pets do estado de São Paulo, 62,5% estão na cidade de São Paulo. A Associação Brasileira da Indústria de Produtos para Animais de Estimação (ABINPET,2013) estima um aumento de 9,2% em relação a 2013, consolidando o país como segundo mercado mundial, perdendo apenas para os Estados Unidos. Em um mercado promissor como este por que ocorre a mortalidade de empresas do ramo? Quais os principais desafios e como contorná-los? O que é necessário fazer para que empreender neste mercado seja algo sustentável ao longo do tempo e quais os pontos devem ser considerados é o que se deseja abordar neste artigo.
Koncepcja polifonicznej powieści reportażowej Laboratorium Reportażu wobec teorii Michaiła Bachtina. Polityczny wymiar polifonii
Authors: Orłowska Paulina
Published: December 2020

Artykuł ma na celu ukazanie zbieżności postulatów Laboratorium Reportażu (LR) z koncepcją polifonii autorstwa Michaiła Bachtina. Jakkolwiek LR nie ma jeszcze na koncie publikacji w pełni polifonicznej, nie oznacza to, że nie dąży do takiego ideału. Zdaniem autora artykułu Marek Miller, twórca LR i główny teoretyk polifonicznej powieści reportażowej, słusznie odwołuje się do myśli rosyjskiego filozofa, dla którego powieść miała wymiar polityczny: była dialogiem przeciwstawionym każdemu typowi metanarracji.
The Vibration of Tubular Beam Conveying Fluid with Variable Cross Section
Authors: Gaith Mohamed
Published: December 2020

The dynamics and stability of flow induced vibration of flow conveying in pipes particularly in case of high velocity flow may lead to severe damage. Predicting the circular natural frequencies and critical fluid velocities is an important tool in design and prevent system failures. In this study transverse dynamic response of simply supported pipe with variable tubular cross sectional area carrying fluid with a constant flow rate is investigated. Euler Bernoulli's beam theory is used to model the pipe. Hamilton's principle will be used to produce the governing equation of motion for the system. The resulting partial differential equation is solved using Galerkin's technique. The impact of the flow velocity and non-uniform variable cross section on the natural frequencies of the system, critical flow velocity and system stability is presented.
The computer-aided system of e-publication contests
Authors: Vlasova S. A. , Kalenov N. E. , Kostyuk K. N.
Published: December 2020

The authors discuss Internet-based contests aimed to reveal high-grade (approved by experts) scientific publications and graduates’ qualifying papers. Such contests are to facilitate science and education. They are held on the federal level or regionally, within an individual discipline or university. Contest performance and objectivity depend significantly on expert board to assess the entries. The modern Inter-net technologies enable to submit digital versions of printed publication and to involve experts of geographically remote regions. This approach is introduced by Direct-Media Publishers that has been the organizer and co-founder of a number of contests of students’, post-graduates’ and scientists’ papers. In 2019, a specialized computer-aided system was designed based on the expert acquisition system operating efficiently at the RAS Library for Natural Sciences. In the expert acquisition system, publications’ information value is assessed by their abstracts with a simple point system. In the contest system, experts shall be submitted to full texts to be assessed in the more complicated way. The system has to be tuned individually to each contest. The system is described in full detail. It supports acquiring and maintaining databases of experts and entries, expertise functionality (study of entries and assessing process), results analysis with marks awarded. The experts database comprises brief information on experts while the entries database comprises publication metadata which enables to identify and classify them within the classification adopted for a contest. The system was tested within 2019 “Science Foundation” and «BeFirst» contests.
Quantifying Engagement with Citations on Wikipedia. (Part 2) (The translation and original text of the article are presented)
Authors: Piccardi T. , West R. , Redi M. , Colavizza G.
Published: December 2020

Wikipedia is one of the most visited sites on the Web and a common source of information for many users. As an encyclopedia, Wikipedia was not conceived as a source of original information, but as a gateway to secondary sources: according to Wikipedia’s guidelines, facts must be backed up by reliable sources that reflect the full spectrum of views on the topic. Although citations lie at the heart of Wikipedia, little is known about how users interact with them. To close this gap, we built client-side instrumentation for logging all interactions with links leading from English Wikipedia articles to cited references during one month, and conducted the first analysis of readers’ interactions with citations. We find that overall engagement with citations is low: about one in 300 page views results in a reference click (0,29% overall; 0,56% on desktop; 0,13% on mobile). Matched observational studies of the factors associated with reference clicking reveal that clicks occur more frequently on shorter pages and on pages of lower quality, suggesting that references are consulted more commonly when Wikipedia itself does not contain the information sought by the user. Moreover, we observe that recent content, open access sources, and references about life events (births, deaths, marriages, etc.) are particularly popular. Taken together, our findings deepen our understanding of Wikipedia’s role in a global information economy where reliability is ever less certain, and source attribution ever more vital.
Mediator Role of the Need for Social Approval in the Relationship between Perfectionism and Codependency: A Structural Equation Modeling Study
Authors: Karaşar Burcu
Published: December 2020

The purpose of the study is to test the model developed in relation to the mediator role of the need for social approval in the relationship between perfectionism and codependency. The study was conducted on a total of 188 students (144 females and 44 males) attending the Education Faculty of Amasya University. In the study, Spann-Fischer Codependency Scale, Need for Social Approval Scale and Frost Multidimensional Perfectionism Scale were used. The theoretical model proposed in the study was tested with the structural equation model. In the analysis of the collected data, LISREL 8.51 was used. The goodness-of-fit indices calculated for the model were found to be good. The goodness-of-fit indices found for the structural model are as follows: RMSEA=0.07, Standardized RMR=0.08, NNFI=0.90, CFI=0.91 and IFI=0.91. It was found that the structural model constructed to reveal the mediator role of the need for social approval in the relationship between perfectionism and codependency has a good fit and the need for social approval was found to have a partial mediator role in the model.
FORMER PLAYERS AS FOOTBALL COACHES IN POLAND
Authors: Metelski Adam , Kaźmierczyk Jerzy
Published: December 2020

Should sports clubs be managed by former professional athletes? There is a belief in the sports world that team management is a mystery, something that only former players can understand. Some studies indeed indicate that practicing sports may actually predispose a person to also performing managerial functions. The aim of the research was to verify if former professional football players do better than other people as coaches of football clubs. The interest in this topic was due to the fact that like a top manager in a company, a coach is a very important figure in the football club and sport is an important part of the global economy today. The research was based on the top Polish football league – Ekstraklasa. The study covered the years 2015-2020 and during this period, a total of 46 head coaches worked in the analyzed clubs. The results indicate that owners of football clubs often hire former successful athletes, and in the research group their average results were better than others. Keywords: former professional players, management in football, team performance
Kabul İle Red Arasında Nüzul’ün Tekrarı Meselesi- II
Authors: Hüseyin Ahmed Abdürrezzak
Published: December 2020

Bu çalışmanın birinci bölümünde teorik olarak mükerrer nüzul meselesi tartışılmıştı. Bu bağlamda tarihsel süreçte konuya yönelik bakış açıları zikredilerek cem’ ve te’līf imkânı var ise önceleneceği; şayet yoksa da tercih kaidelerini dikkate alarak zıt rivayetlerin birinin diğerine tercih edileceği anlatılmıştı. Bununla birlikte klasik ulemadan bazılarının konuyu açıklığa kavuşturmaktan ziyade gelecek nesillere havale ettiği ve bazı çağdaş müelliflerin de konuyu detaylı incelemenin zahmetinden kurtulmak için mükerrer nüzul tanımlamasını kullandıkları da görülmüştü. Çalışmanın bu kısmında ise müellif ilk bölümdeki teorik çerçeveyi bir anlamda mükerrer nüzul’e konu olan iki sūre (Fātiḥa ve İḫlās sūreleri) ile beş ayet grubu (Tevbe 10/113; Hūd 11/114; Naḥl 14/126-128; İsrā 15/85; Rūm 21/1-4)’nda uygulamalı olarak göstermeye çalışmaktadır. Ancak her iki bölümde de bir sorunun cevabı bulunmamaktadır: Cem‘ ve te’līf metodu ile bu problem çözülebiliyorsa bugüne kadar gelen âlimler tarafından neden ertelendi? Çünkü çalışma içinde özellikle geçmiş zamanda yaşamış hadis âlimlerinin değerlendirmelerinden istifade edilmektedir. Bununla birlikte çalışmada kullanılan kaynak ve yöntemler açısından bir yenilik de bulunmamaktadır. Bununla birlikte çalışmada kullanılan kaynak ve yöntemler açısından bir yenilik de bulunmamakta fakat konunun derli toplu sunulduğu ilk müstakil çalışmadır.
Addressing the phytochemical prospection of thermally treated Eucalyptus grandis wood
Authors: Santos Ana Cristina Almeida dos , Souza Natália Dias de , Mesquita Jessica Grama , Santos Junior Alfredo José dos , Sampaio Danielle Affonso , Oliveira Ana Carolina Lindolfo de , Cupertino Gabriela Fontes Mayrinck , Dias Júnior Ananias Francisco
Published: December 2020

This work aimed to investigate phytochemical prospection in treated and untreated wood of Eucalyptus grandis to understand the dynamics of extractives in relation to heat treatment. Samples of E. grandis wood were collected and grouped into external and internal regions. Half of the samples from each region were submitted to heat treatment at 190 ºC. From the treated and untreated samples, phytochemical tests were performed to detect classes of metabolites present in the E. grandis raw wood, hydrophilic extract and lipophilic extract. Phytochemical analysis detected the presence of alkaloids, phenolic compounds and triterpenoids in all hydrophilic extracts of the studied species.  Presence of flavanonols, flavanones and saponins was detected only in the internal region of the wood. Tannins, leucoanthocyanidins, triterpenoids and saponins were influenced by heat treatment. The classes of flavonoids, xanthones and alkaloids are the most resistant to heat treatment. The phytochemical analysis made it possible to identify a new class of extractives that emerged after heat treatment, flavones.
Avaliação dos fatores associados às doenças crônicas não transmissíveis e sua relação com o consumo de vitaminas A, C e E em mulheres em acompanhamento ginecológico preventivo
Authors: Seraglio Heloisa Piva , Lazarotto Angela Khetly , Vieira Valquíria Kulig , Treco Indianara Carlotto , Rech Cinthya Raquel Alba , Wendt Guilherme Welter , Lucio Léia Carolina
Published: December 2020

As Doenças Crônicas Não Transmissíveis (DCNT) são condições que impactam na qualidade de vida da população. As vitaminas antioxidantes - dentre elas as vitaminas A, C e E - são micronutrientes que regulam o processo de estresse oxidativo, presente na etiologia dessas doenças. Portanto, o objetivo deste estudo quantitativo e transversal foi de avaliar os fatores associados com diabetes mellitus tipo 2, hipertensão arterial sistêmica e obesidade, sobretudo aqueles fatores ligados com o consumo de vitaminas antioxidantes em 112 mulheres em acompanhamento ginecológico preventivo em unidade de referência oncológica da cidade de Francisco Beltrão, PR, Brasil.  Para a coleta dos dados, foi efetuada a aplicação de dois questionários, visando avaliar o estado nutricional e de saúde das participantes, dados antropométricos, informações socioeconômicas, presença de comorbidades e o consumo de fontes alimentares das vitaminas antioxidantes por meio do Questionário de Frequência Alimentar (QFA). Os resultados mostraram que 8,9% da população estudada apresentou DM2, possuindo como fatores associados a idade mínima de 50 anos, presença de HAS e consumo de vitamina A. Em se tratando da HAS, verificou-se uma prevalência de 32,1% e o único fator efetivamente associado foi a presença de DM2. Já para a obesidade, não foi observado nenhum fator significativo associado a ela. O presente estudo reforça as evidências de que a ingestão adequada de frutas e hortaliças, por serem fontes de nutrientes antioxidantes, deve ser estimulada para prevenir as DCNT. No entanto, é necessário ampliar os estudos sobre a sua relação com essas patologias de forma mais generalista.
Uso de antibióticos na insuficiência renal: necessidade de ajustes posológicos e doses individualizadas
Authors: Marques Fabiana Espindola , Souza Olívia Raquel Pereira de , Bezerra Jeamile Lima , Sousa Jailson Carmo de , Andrade Sâmia Moreira de , Cunha Maurício Almeida , Nery Neto José Arimatéa de Oliveira , Taminato Rodrigo Luís , Oliveira Evaldo Hipólito de
Published: December 2020

A alteração farmacocinética e farmacodinâmica de medicamentos, em virtude de comprometimento da função renal, é frequentemente observada. Segundo a Organização Mundial da Saúde - OMS (2015), é possível perder até 90% da função renal antes de manifestar qualquer sintoma. O objetivo deste trabalho foi confeccionar uma tabela prática para consulta de antibióticos que necessitam de ajustes em pacientes com insuficiência renal. Realizou-se um estudo documental, descritivo e analítico com abordagem qualitativa a partir da análise da farmacocinética dos antibióticos padronizados do Hospital Universitário do Piauí buscando identificar a necessidade de ajustes de doses e frequências de uso conforme a função renal do paciente. Para a execução do estudo foram consultadas referências descritas em bulas para profissionais da saúde, sendo as informações comparadas às referências descritas em guias farmacoterapêuticos dos principais hospitais de excelência do Brasil segundo o Ministério da Saúde, e o guia Standford, além de revisões de literatura. Os antimicrobianos foram organizados por classe farmacológica, descrevendo o ajuste da dose, a depender do clearence de creatina, e dose suplementar após hemodiálise. Apesar das orientações a respeito do ajuste posológico em pacientes com insuficiência renal, permanecem ainda dúvidas, pois existem outros fatores que influenciam nessa correção como o método dialítico empregado na hemodiálise que compreende: intermitente ou convencional, ultrafiltração, diálise de baixa eficiência. O presente trabalho gerou uma tabela de consulta rápida com finalidade de esclarecer dúvidas na prescrição ou dispensação dos antimicrobianos padronizados no hospital, promovendo assim terapêutica antimicrobiana mais segura e eficiente.
Detecção de resíduos de antibióticos em leite cru, pasteurizado e Ultra High Temperature (UHT) comercializados no município de Unaí, Minas Gerais
Authors: Caldeira Bruna Ranne Mendes , Sola Marília Cristina
Published: December 2020

A qualidade do leite pode ser avaliada por parâmetros físico-químicos, sensoriais, microbiológicos e também pela presença de resíduos químicos, como os antimicrobianos. A principal fonte destas substâncias no leite é consequência do manejo inadequado de medicamentos visando o controle de enfermidades no rebanho, descumprimento no descarte de leite durante o período de carência e falhas na higienização de utensílios e equipamentos. Buscou-se neste estudo avaliar a qualidade do leite cru, pasteurizado e UHT comercializados no município de Unaí-MG sob fiscalização dos serviços de inspeção ou comercializados informalmente, quanto à presença de resíduos de antibióticos. Foram analisadas 56 amostras de leite, sendo 16 de leite cru, 16 de leite pasteurizado integral e 24 amostras de leite UHT (8 amostras de leite integral, 8 de semidesnatado e 8 amostras de leite desnatado) a partir do emprego do kit IDEXX SNAP® duo™ ST Plus. Após a realização dos ensaios analíticos foi possível verificar que as amostras de leite pasteurizado e UHT não continham resíduos dos antibióticos testados, enquanto apenas uma amostra de leite cru apresentou-se positiva para a presença de resíduos de β-lactâmicos. Estes resultados indicam a necessidade do cumprimento do período de carência durante o tratamento de animais de produção bem como a necessidade de controle de resíduos de antimicrobianos nas plataformas de recepção de indústrias de laticínios, além da fiscalização por parte do Serviço de Inspeção Oficial visando a produção de alimentos inócuos.
Expression of HIF-1α and VEGF in Skeletal Muscle of Plateau Animals in Response to Hypoxic Stress
Authors: XIE H.-C. , HE J.-P. , ZHU J.-F. , LI J.-G.
Published: December 2020

Hypoxia-inducible factor-1α (HIF-1α) transcriptionally regulates expression of several target genes in protecting tissues against hypoxia. With hypoxic stress, vascular endothelial growth factor (VEGF) is a signal protein produced by cells and further contributes to improvement of vascular functions and restoring the oxygen supply to tissues. In this current study, we first hypothesized that the protein levels of HIF-1α and VEGF are reduced in skeletal muscles of plateau animals [China Qinghai-Tibetan plateau pikas (ochotona curzoniae)] in response to hypoxia as compared with control animals [normal lowland Sprague-Dawley (SD) rats]. We further hypothesized that HIF-1α plays a role in regulating expression of VEGF in skeletal muscle. Note that HIF-1α and VEGF were determined by using two-site immunoenzymatic assay (ELISA) methods. Our results demonstrated that hypoxic stress induced by exposure of lower O2 (6 h) significantly increased the levels of HIF-1α and VEGF in the oxidative and glycolytic muscles of SD rats and pikas (P<0.05 vs. normoxic conditions). Notably, the increases in HIF-1α and VEGF were significantly less in pikas (P<0.05, vs. SD controls) than in SD rats. In addition, a linear relationship was observed between amplified HIF-1α and VEGF in oxidative muscle (r=0.76 and P<0.01) and glycolytic muscle (r=0.72 and P<0.01) and inhibiting HIF-1α significantly decreased expression of VEGF induced by hypoxic stress in skeletal muscles (P<0.05). Overall, our findings suggest that (1) responsiveness of HIF-1α and VEGF in skeletal muscles to hypoxic stress is blunted in plateau animals, and (2) HIF-1α has a regulatory effect on VEGF under hypoxic environment.
Molecular Genetic Identification of Populations of Pinus sylvestris L. and Larix sibirica Ledeb. in Perm Krai Using SNP-markers
Authors: Chertov N. , Pystogova N. , Malyshkina E. , Nechaeva Yu. , Boronnikova S. , Kalendar R.
Published: December 2020

For the selection of identification SNP markers and identification at the population level, 5 populations of Scots pine (Pinus sylvestris L., Pinaceae) and 5 populations of the western race of Siberian larch (Larix sibirica Ledeb., Pinaceae) in the Perm Krai were studied. To determine nucleotide sequences and select identification SNP markers in 10 populations of two woody plant species of the Perm Krai, 14 pairs of nuclear and 3 pairs of chloroplast loci of P. sylvestris, as well as ten pairs of primers to 10 loci of potentially adaptively significant genes of L. sibirica were tested. Sequencing of 3 nuclear loci and 3 loci of chloroplast DNA of P. sylvestris, as well as 6 loci of nuclear adaptively significant genes of L. sibirica were sequenced. In the sequenced sequences of two plant species, 59 SNP markers were detected. Of these, 11 identification SNP markers were established, with a sufficiently high frequency of occurrence (≥0.5), suitable for identifying populations of two coniferous plant species.
The Antioxidant Enzymes Activity From the Poly-extromophilic Yarrowia lipolytica Yeast Under Oxidative Stress During Long-lasting Cultivation
Authors: Sekova V. , Bobrova E. , Isakova E. , Deryabina Yu.
Published: December 2020

Hydrogen peroxide is one of the most widespread reactive oxygen species, which can diffuse through cell membranes, causing changes in the redox status of cells and the development of oxidative stress. The results show that the effects caused by hydrogen peroxide are dose-dependent and can lead to both damage to cells and an increase in their resistance to oxidative stress. In this study, we assayed the effect of various concentrations of H2O2 on the redox status of the Yarrowia lipolytica yeast during long-lasting cultivation. The oxidant application to the cells in the logarithmic growth stage was shown to delay the impact on the ROS level in the late stationary growth stage. In this case, the dependence of the injected concentration on the redox status is not linear, which suggests triggering different signaling mechanisms by various concentrations of the oxidant.
EVALUACIÓN DE LA CALIDAD DE LA ATENCIÓN EN RELACIÓN CON LA SATISFACCIÓN DE LOS USUARIOS DE MEDICINA INTERNA EN EL HOSPITAL BÁSICO DE GUARANDA DEL INSTITUTO ECUATORIANO DE SEGURIDAD SOCIAL
Authors:   Universidad Estatal de Bolívar, Arguello Pazmiño Alexandra Maribel , Monar Solórzano Manuel Mesías ,   Universidad Estatal de Bolívar, Argüello Pazmiño Verónica Janeth ,   Universidad Estatal de Bolívar, Chávez García Elsita Margoth ,   Universidad Estatal de Bolívar
Published: December 2020

La calidad de la atención en los servicios de salud pública se ha convertido en un tema de debate académico al existir diversos enfoques para su medición, sin embargo, la demanda en servicios de salud ha venido incrementándose en diversos países. Ecuador dispone de una red de hospitales públicos, cuya política es brindar un servicio de calidad de vida a los ciudadanos. El objetivo es explorar la fiabilidad y validez del cuestionario adaptado SERVPERF en el ámbito de atención de medicina interna en el hospital básico de Guaranda del Instituto Ecuatoriano de Seguridad Social. La muestra fue de 289 de acuerdo a los datos proporcionados del Instituto de acuerdo al número de egresos reportados en el año 2018. El diseño de la investigación es positivista de tipo transversal con un enfoque deductivo para el desarrollo de la teoría, a partir de un método cuantitativo. Con el instrumento aplicado se midió la percepción de los usuarios en la calidad de atención en medicina interna percibida por los usuarios, se utilizó la encuesta adaptada SERVPERF, con 22 ítems medidos en escala de likert (1-7), a las que 3 preguntas abiertas y variables sociodemográficas. Se determinó que el modelo
ESTUDIO DEL FENÓMENO DE LAS ABUELAS Y LOS ABUELOS CUIDADORES.
Authors:   Universidad Técnica de Ambato, Agila Tene Cinthya Elizabeth , Balseca Basantes Jeanneth Elizabeth ,   Universidad Técnica de Ambato
Published: December 2020

El presente estudio tiene como objetivo principal diagnosticar la situación actual de las abuelas y los abuelos cuidadores de distintas zonas tanto urbana como rural del cantón Ambato de la provincia de Tungurahua, con el fin de obtener una concreta caracterización del rol del abuelo cuidador. Como investigación básica se utilizó el método deductivo con un enfoque cuantitativo y un nivel de investigación descriptiva. El universo de la población fue de 1360 adultos mayores pertenecientes a los grupos MIES e IESS y en la muestra participaron 300 abuelos, entre ellos 236 mujeres y 64 hombres. Dentro de los principales resultados son: la actividad laboral de los padres (86,00%) es la causa principal para que los abuelos asuman el rol de cuidador. Las actividades que desarrollan los abuelos durante el cuidado a sus nietos son: 77,33% en preparar alimentos, 49,67% en cuidar a sus nietos cuando se enferman y 34,67% en actividades recreativas. Por último, el máximo tiempo de cuidados es 12 años en adelante con 35,00%. Se concluye destacando el nuevo papel del abuelo en la familia como padres sustitutos al permanecer tantos años con sus nietos y se identifica sobrecarga al realizar las actividades de cuidado. Pala
PENGARUH ARUS KAS OPERASI, ARUS KAS INVESTASI, DAN ARUS KAS PENDANAAN TERHADAP RETURN SAHAM PADA PERUSAHAAN MANUFAKTUR YANG TERDAFTAR DI BEI PERIODE 2014-2019
Authors: Harahap Baru , Effendi Syahril
Published: December 2020

This study aims to determine the effect of cash flows from operating, investing, and financing to saham Return. Data obtained from the income statement, cash flow statement, as well as saham market prices daily on Textile companies listed on the Saham Exchange 2014-2019. The analytical tool used is multiple linear regression, F test and t test. The samples are 15 companies Textile sector during the period 2014-2019. Based on the survey results revealed that a significant difference between cash flows from operating, investing, and financing on saham Returns with sig <0.05. T test results of each independent variable cash flows from operating, investing, and financing an effect on saham Returns (Returns) with sig < 0.05. The conclusion that can be drawn is that the investors who want to invest their shares in the company on the Indonesian Saham Exchange should pay attention to the ratio of a company's cash investment object, because of the results showed that the cash flow effect on saham Returns
Cartilage Extracellular Matrix Scaffold With Kartogenin-Encapsulated PLGA Microspheres for Cartilage Regeneration
Authors: Zhao Yanhong , Zhao Xige , Zhang Rui , Huang Ying , Li Yunjie , Shan Minhui , Zhong Xintong , Xing Yi , Wang Min , Zhang Yang , Zhao Yanmei
Published: December 2020

Repair of articular cartilage defects is a challenging aspect of clinical treatment. Kartogenin (KGN), a small molecular compound, can induce the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. Here, we constructed a scaffold based on chondrocyte extracellular matrix (CECM) and poly(lactic-co-glycolic acid) (PLGA) microspheres (MP), which can slowly release KGN, thus enhancing its efficiency. Cell adhesion, live/dead staining, and CCK-8 results indicated that the PLGA(KGN)/CECM scaffold exhibited good biocompatibility. Histological staining and quantitative analysis demonstrated the ability of the PLGA(KGN)/CECM composite scaffold to promote the differentiation of BMSCs. Macroscopic observations, histological tests, and specific marker analysis showed that the regenerated tissues possessed characteristics similar to those of normal hyaline cartilage in a rabbit model. Use of the PLGA(KGN)/CECM scaffold may mimic the regenerative microenvironment, thereby promoting chondrogenic differentiation of BMSCs in vitro and in vivo. Therefore, this innovative composite scaffold may represent a promising approach for acellular cartilage tissue engineering.
Simultaneous Optimization of Elastic-Plastic Building Structures and Viscous Dampers Under Critical Double Impulse
Authors: Akehashi Hiroki , Takewaki Izuru
Published: December 2020

A new method for simultaneous optimal design of main building structures and viscous dampers is proposed for elastic-plastic multi-degree-of-freedom (MDOF) building structures subjected to the critical double impulse which is regarded as a representative of the main part of near-fault ground motions. The critical double impulse is characterized by the maximum energy input to the total system by the second impulse and the sum of the restoring force and the damping force in the first story attains zero by this critical input. The objective function is the maximum interstory drift along the building height. The original optimization problem is transformed into a problem of removing the most inactive story stiffness and damper damping coefficient. An efficient sensitivity-based design algorithm is developed for this simultaneous optimal design problem of main building structures and viscous dampers. It is pointed out that the order of changes of structural stiffness and damper damping magnitude is critical to the achievement of reasonable designs and cycle-by-cycle alternating redesign of story stiffness and damper damping coefficient is effective for its achievement. The double impulse pushover (DIP) analysis proposed in the previous paper (Akehashi and Takewaki, 2019) for determining the input velocity level of the critical double impulse is also conducted to disclose the response characteristics of the designed building structures and dampers. It is shown that the proposed design method enables the high yield-strength design with effective seismic energy absorption and the high limit-strength design effective for extremely large disturbances. The distributions of the maximum acceleration responses in an initial design and the final design are also presented for the one-cycle sine wave corresponding to the critical double impulse.
REX-001, a BM-MNC Enriched Solution, Induces Revascularization of Ischemic Tissues in a Murine Model of Chronic Limb-Threatening Ischemia
Authors: Rojas-Torres Marta , Jiménez-Palomares Margarita , Martín-Ramírez Javier , Beltrán-Camacho Lucía , Sánchez-Gomar Ismael , Eslava-Alcon Sara , Rosal-Vela Antonio , Gavaldá Sandra , Durán-Ruiz Mª Carmen
Published: December 2020

Background: Bone Marrow Mononuclear Cells (BM-MNC) constitute a promising alternative for the treatment of Chronic Limb-Threatening ischemia (CLTI), a disease characterized by extensive blockade of peripheral arteries, clinically presenting as excruciating pain at rest and ischemic ulcers which may lead to gangrene and amputation. BM-MNC implantation has shown to be efficient in promoting angiogenesis and ameliorating ischemic symptoms in CLTI patients. However, the variability seen between clinical trials makes necessary a further understanding of the mechanisms of action of BM-MNC, and moreover, to improve trial characteristics such as endpoints, inclusion/exclusion criteria or drug product compositions, in order to implement their use as stem-cell therapy.

Materials: Herein, the effect of REX-001, a human-BM derived cell suspension enriched for mononuclear cells, granulocytes and CD34+ cells, has been assessed in a murine model of CLTI. In addition, a REX-001 placebo solution containing BM-derived red blood cells (BM-RBCs) was also tested. Thus, 24 h after double ligation of the femoral artery, REX-001 and placebo were administrated intramuscularly to Balb-c nude mice (n:51) and follow-up of ischemic symptoms (blood flow perfusion, motility, ulceration and necrosis) was carried out for 21 days. The number of vessels and vascular diameter sizes were measured within the ischemic tissues to evaluate neovascularization and arteriogenesis. Finally, several cell-tracking assays were performed to evaluate potential biodistribution of these cells.

Results: REX-001 induced a significant recovery of blood flow by increasing vascular density within the ischemic limbs, with no cell translocation to other organs. Moreover, cell tracking assays confirmed a decrease in the number of infused cells after 2 weeks post-injection despite on-going revascularization, suggesting a paracrine mechanism of action.

Conclusion: Overall, our data supported the role of REX-001 product to improve revascularization and ischemic reperfusion in CLTI.
Cyclic Stretch Induces Vascular Smooth Muscle Cells to Secrete Connective Tissue Growth Factor and Promote Endothelial Progenitor Cell Differentiation and Angiogenesis
Authors: Yan Jing , Wang Wen-Bin , Fan Yang-Jing , Bao Han , Li Na , Yao Qing-Ping , Huo Yun-Long , Jiang Zong-Lai , Qi Ying-Xin , Han Yue
Published: December 2020

Endothelial progenitor cells (EPCs) play a vital role in endothelial repair following vascular injury by maintaining the integrity of endothelium. As EPCs home to endothelial injury sites, they may communicate with exposed vascular smooth muscle cells (VSMCs), which are subjected to cyclic stretch generated by blood flow. In this study, the synergistic effect of cyclic stretch and communication with neighboring VSMCs on EPC function during vascular repair was investigated. In vivo study revealed that EPCs adhered to the injury site and were contacted to VSMCs in the Sprague–Dawley (SD) rat carotid artery injury model. In vitro, EPCs were cocultured with VSMCs, which were exposed to cyclic stretch at a magnitude of 5% (which mimics physiological stretch) and a constant frequency of 1.25 Hz for 12 h. The results indicated that stretched VSMCs modulated EPC differentiation into mature endothelial cells (ECs) and promoted angiogenesis. Meanwhile, cyclic stretch upregulated the mRNA expression and secretion level of connective tissue growth factor (CTGF) in VSMCs. Recombinant CTGF (r-CTGF) treatment promoted endothelial differentiation of EPCs and angiogenesis, and increased their protein levels of FZD8 and β-catenin. CTGF knockdown in VSMCs inhibited cyclic stretch-induced EPC differentiation into ECs and attenuated EPC tube formation via modulation of the FZD8/β-catenin signaling pathway. FZD8 knockdown repressed endothelial differentiation of EPCs and their angiogenic activity. Wnt signaling inhibitor decreased the endothelial differentiation and angiogenetic ability of EPCs cocultured with stretched VSMCs. Consistently, an in vivo Matrigel plug assay demonstrated that r-CTGF-treated EPCs exhibited enhanced angiogenesis; similarly, stretched VSMCs also induced cocultured EPC differentiation toward ECs. In a rat vascular injury model, r-CTGF improved EPC reendothelialization capacity. The present results indicate that cyclic stretch induces VSMC-derived CTGF secretion, which, in turn, activates FZD8 and β-catenin to promote both differentiation of cocultured EPCs into the EC lineage and angiogenesis, suggesting that CTGF acts as a key intercellular mediator and a potential therapeutic target for vascular repair.
BMSC-Derived Exosomal miR-29a Promotes Angiogenesis and Osteogenesis
Authors: Lu Guo-dong , Cheng Peng , Liu Ting , Wang Zhong
Published: December 2020

Angiogenesis and osteogenesis are tightly coupled during bone modeling and remodeling processes. Here we reported that bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-29a promotes angiogenesis and osteogenesis in vitro and in vivo. BMSC-derived exosomes (BMSCs-Exos) can be taken up by human umbilical vein endothelial cells (HUVECs) and promote the proliferation, migration, and tube formation of HUVECs. MiRNA-29a level was high in BMSCs-Exos and can be transported into HUVECs to regulate angiogenesis. VASH1 was identified as a direct target of miR-29a, mediating the effects of BMSC-derived exosomal miR-29a on angiogenesis. More interestingly, miR29a-loaded exosomes from engineered BMSCs (miR-29a-loaded BMSCs-Exos) showed a robust ability of promoting angiogenesis and osteogenesis in vivo. Taken together, these findings suggest that BMSC-derived exosomal miR-29a regulates angiogenesis and osteogenesis, and miR-29a-loaded BMSCs-Exos may serve as a potential therapeutic target for osteoporosis.