bluuebunny/crossref_metadata_2025 huggingface dataset:
Parallel Computation of CRC-Code on an FPGA Platform for High Data Throughput
Authors: Tran Dat , Aslam Shahid , Gorius Nicolas , Nehmetallah George
Published: April 2021

With the rapid advancement of radiation hard imaging technology, space-based remote sensing instruments are becoming not only more sophisticated but are also generating substantially more amounts of data for rapid processing. For applications that rely on data transmitted from a planetary probe to a relay spacecraft to Earth, alteration or discontinuity in data over a long transmission distance is likely to happen. Cyclic Redundancy Check (CRC) is one of the most well-known package error check techniques in sensor networks for critical applications. However, serial CRC computation could be a bottleneck of the throughput in such systems. In this work, we design, implement, and validate an efficient hybrid look-up-table and matrix transformation algorithm for high throughput parallel computational unit to speed-up the process of CRC computation using both CPU and Field Programmable Gate Array (FPGA) with comparison of both methods.
Design and Implement of Three-Phase Permanent-Magnet Synchronous Wave Generator using Taguchi Approach
Authors: Hsiao Chun-Yu , Lai Chin-Hsiang , Zheng Zhu-Xuan , Li Guan-Yu
Published: April 2021

In this paper, the design and performance analysis of a high-efficiency permanent-magnet synchronous wave generator (PSWG) are presented. A systematic approach for the design of the outer rotor was proposed as a prototype model. The magnetic field, magnetic circuit characteristics, electrical characteristics of the generator, and optimal design parameters such as the pole–arc ratio and shoe outer length were determined using the Taguchi method, finite-element analysis (FEA) software, and rotor skewing techniques. The proposed six series and six parallel-connection winding configurations can provide an evenly distributed current for practical applications. A PSWG was designed and fabricated according to the proposed methodology. According to the experimental results by implementing the optimized design, the efficiencies of the proposed PSWG which used 3.6 Ω load at 300 rpm is 86.32% and the efficiency error between simulation and experiment is less than 1.8%. It verifies the feasibility of the proposed method to PSWG and the structural reliability optimization design.
Development of New Mean of Individual Transport for Application in Underground Coal Mines
Authors: Kamiński Paweł
Published: April 2021

Development of underground coal mines is reflected in increasing lengths of transport routes between shafts and workplaces. This is not an issue in terms of transportation of materials or groups of people, because suspended railways are efficient, safe, and fast mean of transport. However, transportation of individual people or small loads is an issue because of the lack of a means of individual transport and the high cost of transport using suspended railways. The development of individual means of transport, based on historical solutions, utilizes existing equipment of mine workings. This paper presents the stages of development of an underground bike, from the original idea to tests and analysis. The development process consists of simulations of different parameters of the bike using Working Model software and real-life tests conducted in an underground mine. The paper also presents analysis of the legal background of this new means of individual transport.
Thermal Performance Analysis of Heat Collection Wall in High-Rise Building Based on the Measurement of Near-Wall Microclimate
Authors: Li Ruixin , Zhao Yiwan , Lv Gaochong , Li Weilin , Zhu Jiayin , Bantserova Olga L.
Published: April 2021

Near-wall microenvironment of a building refers to parameters such as wind speed, temperature, relative humidity, solar radiation near the building’s façade, etc. The distribution of these parameters on the building façade shows a certain variation based on changes in height. As a technology of passive heating and ventilation, the effectiveness of this application on heat collection wall is significantly affected by the near-wall microclimate, which is manifested by the differences, and rules of the thermal process of the components present at different elevations. To explore the feasibility and specificity of this application of heat collection wall in high-rise buildings, this study uses three typical high-rise buildings from Zhengzhou, China, as research buildings. Periodic measurements of the near-wall microclimate during winter and summer were carried out, and the changing rules of vertical and horizontal microclimate were discussed in detail. Later, by combining these measured data with numerical method, thermal process and performance of heat collection wall based on increasing altitude were quantitatively analyzed through numerical calculations, and the optimum scheme for heat collection wall components was summarized to provide a theoretical basis for the structural design of heat-collecting wall in high-rise buildings.
Growth Relationships in Silver Fir Stands at Their Lower-Altitude Limit in Romania
Authors: Tudoran Gheorghe-Marian , Cicșa Avram , Ciceu Albert , Dobre Alexandru-Claudiu
Published: April 2021

This study presents the biometric relationships among various increments that is useful in both scientific and practical terms for the silvicultural of silver fir. The increments recorded in the biometric characteristics of trees are a faithful indicator of the effect of silvicultural work measures and of environmental conditions. Knowing these increments, and the relationships among them, can contribute to adaptations in silvicultural work on these stands with the purpose of reducing risks generated by environmental factors. We carried an inventory based on tree increment cores. The sample size was determined based on both radial increment and height increment variability of the trees. The sample trees were selected in proportion to their basal area on diameter categories. Current annual height increment (CAIh) was measured on felled trees from mean tree category. For CAIh we generated models based on the mean tree height. Percentages of the basal area increment and of form-height increment were used to compute the current annual volume increment percentage (PCAIv). For the mean tree, the CAIh estimated through the used models had a root-mean-square error (RMSE) of 0.8749 and for the current annual volume increment (CAIv) the RMSE value was 0.1295. In even-aged stands, the mean current volume increment tree is a hypothetical tree that may have the mean basal area of all the trees and the form-height of the stand. Conclusions: The diameter, height, and volume increments of trees are influenced by structural conditions and natural factors. The structures comprising several generations of fir mixed with beech and other deciduous trees, which have been obtained by the natural regeneration of local provenances, are stable and must become management targets. Stable structures are a condition for the sustainable management of stands.
Nitrogen Fertilization Increases Windstorm Damage in an Aggrading Forest
Authors: Walter Christopher A. , Fowler Zachariah K. , Adams Mary Beth , Burnham Mark B. , McNeil Brenden E. , Peterjohn William T.
Published: April 2021

Storms are the most significant disturbance events in temperate forests. Forests impacted by nitrogen deposition may face more severe storm damage as changes in soil and wood chemistry impact tree growth allocation, wood strength, and species composition. To examine these potential effects of nitrogen deposition, we measured tree damage from a windstorm in an aggrading forest that is part of a nitrogen fertilization experiment. We discovered that within the nitrogen fertilization treatment area there was significantly more basal area and stems damaged when compared to the reference treatment, and the nitrogen fertilization treatment had more snapped and severely damaged trees. Additionally, the effect of treatment and amount of damage to trees was different depending on tree species. If our results are indicative of the large and globally-distributed regions of temperate forests impacted by nitrogen deposition, then the increased windstorm disturbance risk posed by climate change could be more significant due to the effects of nitrogen deposition.
An Experimental Education Project for Consultations of Older Adults during the Pandemic and Healthcare Lockdown
Authors: Neumann-Podczaska Agnieszka , Seostianin Mikołaj , Madejczyk Konrad , Merks Piotr , Religioni Urszula , Tomczak Zofia , Tobis Sławomir , Moga Daniela Claudia , Ryan Melody , Wieczorowska-Tobis Katarzyna
Published: April 2021

Objective: To develop a mentor-supervised, interprofessional, geriatric telemedicine experiential education project in response to the COVID-19 pandemic. Method: Medical and pharmacy students collaborated via remote consultations to address the coexistence of multimorbidity and polypharmacy in geriatric patients. In-depth interviews of students and patients as well as Likert scale-based telephonic survey were performed for a comprehensive evaluation of the project’s significance. Results: To date, 49 consultations have been conducted. Remote consultations performed by medical and pharmacy students working collaboratively were beneficial for both students, participants. Conclusions and Practice Implications: This experimental education project provided students with authentic challenges while simultaneously delivering care to the older adults who are susceptible to disruption of care associated with the pandemic. Further development and expanded implementation of such approaches may be a post-pandemic practice to provide more accessible care for senior patients while incorporating interprofessional education.
Weekly Question-and-Answer Extension Radio Show Helps Listeners Adopt Environmentally Sound Horticulture Practices
Authors: Weisenhorn Julie , Meyer Mary , McGoff Regina , Rooney Theresa
Published: April 2021

Cooperative Extension has a long history of using radio broadcasts in educational programming. The Minneapolis, MN-based CBS affiliate WCCO Radio 830AM contacted the University of Minnesota Extension (UMNExt) in 2013 to reinstate an 8:00 AM Saturday live gardening show titled “Smart Garden”. After several years of doing the radio show, we wanted to determine the effectiveness of getting information to listeners, what people were doing differently because of what they heard on the show, and how much they used the Extension’s resources after listening to the program. After analyzing 410 responses to an online survey, we found 78% of respondents reported they frequently or always learned something new when listening, and 56% reported adopting environmentally important practices, such as pollinator gardens, less chemical use, better lawn care, water conservation, and removing invasive plants. We concluded that this educational programming was making a positive impact on consumer horticulture practices.
Health-Related Quality of Life among Cancer Survivors Depending on the Occupational Status
Authors: Kim Kisook , Yoon Hyohyeon
Published: April 2021

The study aimed to identify and compare the factors affecting health-related quality of life (HRQoL) depending on the occupational status of cancer survivors. This study was a secondary data analysis from the Korea National Health and Nutrition Examination Survey (KNHANES) from 2014 to 2018. Hierarchical multivariate linear regression was used to investigate the factors affecting the HRQoL of each group. Non-working cancer survivors had significantly lower HRQoL than working cancer survivors (p < 0.001). A hierarchical multiple regression model showed that demographic, health-related, and psychological characteristics explained 62.0% of non-working cancer survivors’ HRQoL (F = 4.29, p < 0.001). Among the input variables, health-related characteristics were the most influential factors (ΔR2 = 0.274, F = 9.84, p < 0.001). For working cancer survivors, health-related characteristics were the only variable that was statistically associated with HRQoL (F = 5.556, p < 0.001). It is important to enhance physical activities and manage the chronic disease to improve the HRQoL of working cancer survivors. Further, managing health-related characteristics, including depressive symptoms and suicidal ideation, is necessary for non-working cancer survivors. Regarding working survivors, psychological factors such as depressive symptoms and suicidal tendencies did not affect HRQoL. Therefore, an early and effective return to work program should be developed for the improvement of their HRQoL.
Advancement in Benthic Microbial Fuel Cells toward Sustainable Bioremediation and Renewable Energy Production
Authors: Umar Mohammad Faisal , Rafatullah Mohd , Abbas Syed Zaghum , Mohamad Ibrahim Mohamad Nasir , Ismail Norli
Published: April 2021

Anthropogenic activities are largely responsible for the vast amounts of pollutants such as polycyclic aromatic hydrocarbons, cyanides, phenols, metal derivatives, sulphides, and other chemicals in wastewater. The excess benzene, toluene and xylene (BTX) can cause severe toxicity to living organisms in wastewater. A novel approach to mitigate this problem is the benthic microbial fuel cell (BMFC) setup to produce renewable energy and bio-remediate wastewater aromatic hydrocarbons. Several mechanisms of electrogens have been utilized for the bioremediation of BTX through BMFCs. In the future, BMFCs may be significant for chemical and petrochemical industry wastewater treatment. The distinct factors are considered to evaluate the performance of BMFCs, such as pollutant removal efficiency, power density, and current density, which are discussed by using operating parameters such as, pH, temperature and internal resistance. To further upgrade the BMFC technology, this review summarizes prototype electrode materials, the bioremediation of BTX, and their applications.
Toxic Metals in Cereals in Cape Verde: Risk Assessment Evaluation
Authors: Rubio-Armendáriz Carmen , Paz Soraya , Gutiérrez Ángel J. , Gomes Furtado Verena , González-Weller Dailos , Revert Consuelo , Hardisson Arturo
Published: April 2021

Consumption of cereals and cereal-based products represents 47% of the total food energy intake in Cape Verde. However, cereals also contribute to dietary exposure to metals that may pose a risk. Strengthening food security and providing nutritional information is a high-priority challenge for the Cape Verde government. In this study, toxic metal content (Cr, Ni, Sr, Al, Cd, and Pb) is determined in 126 samples of cereals and derivatives (rice, corn, wheat, corn flour, wheat flour, corn gofio) consumed in Cape Verde. Wheat flour samples stand out, with the highest Sr (1.60 mg/kg), Ni (0.25 mg/kg) and Cr (0.13 mg/kg) levels. While the consumption of 100 g/day of wheat would contribute to 13.2% of the tolerable daily intake (TDI) of Ni, a consumption of 100 g/day of wheat flour would contribute to 8.18% of the tolerable weekly intake (TWI) of Cd. Results show relevant Al levels (1.17–13.4 mg/kg), with the highest level observed in corn gofio. The mean Pb average content in cereals is 0.03–0.08 mg/kg, with the highest level observed in corn gofio. Al and Pb levels are lower in cereals without husks. Without being a health risk, the consumption of 100 g/day of wheat contributes to 17.5% of the European benchmark doses lower confidence limit (BMDL) of Pb for nephrotoxic effects; the consumption of 100 g/day of corn gofio provides an intake of 1.34 mg Al/day (13.7% of the TWI) and 8 µg Pb/day (20% of the BMDL for nephrotoxic effects). A strategy to minimize the dietary exposure of the Cape Verdean population to toxic metals from cereals should consider the continuous monitoring of imported cereals on arrival in Cape Verde, the assessment of the population’s total diet exposure to toxic metals and educational campaigns.
Teaching Methodologies and School Organization in Early Childhood Education and Its Association with Physical Activity
Authors: Nielsen-Rodríguez Adriana , Romance Ramón , Dobado-Castañeda Juan Carlos
Published: April 2021

Early childhood represents a crucial period for child development. Physical activity is essential in this process, but studies show that children are very inactive and do not meet the recommended minimums. Due to the large proportion of time they spend at school, it is necessary to examine active and sedentary behaviors in these environments. The aim of the study is to analyze the amount and intensity of physical activity in preschool children during the school day according to the methodology used. Using accelerometry, the amount and intensity of physical activity and sedentary behavior of 156 children aged 4–6 years at different times of the school day were evaluated. The results revealed that preschoolers spend most of their class time sedentary, with children participating in active methodologies registering the highest amount and intensity of physical activity. Recess and specific motor sessions are the most active times, although the latter should increase the time of intense activity that they imply. To increase physical activity during the school day, it is necessary to establish movement integration methodologies, while increasing the number and adjusting the duration of specific motor sessions and of recesses, so that the maximum possible use is made of them.
The Psychosocial and Somatic Effects of Relocation from Remote Canadian First Nation Communities to Urban Centres on Indigenous Peoples with Chronic Kidney Disease (CKD)
Authors: Genereux Denise , Fan Lida , Brownlee Keith
Published: April 2021

Chronic kidney disease, also referred to as end-stage renal disease (ESRD), is a prevalent and chronic condition for which treatment is necessary as a means of survival once affected individuals reach the fifth and final stage of the disease. Dialysis is a form of maintenance treatment that aids with kidney functioning once a normal kidney is damaged. There are two main types of dialysis: hemodialysis (HD) and peritoneal dialysis (PD). Each form of treatment is discussed between the patient and nephrologist and is largely dependent upon the following factors: medical condition, ability to administer treatment, supports, geographical location, access to necessary equipment/supplies, personal wishes, etc. For Indigenous Peoples who reside on remote Canadian First Nation communities, relocation is often recommended due to geographical location and limited access to both health care professionals and necessary equipment/supplies (i.e., quality of water, access to electricity/plumbing, etc.). Consequently, the objective of this paper is to determine the psychosocial and somatic effects for Indigenous Peoples with ESRD if they have to relocate from remote First Nation communities to an urban centre. A review of the literature suggests that relocation to urban centres has negative implications that are worth noting: cultural isolation, alienation from family and friends, somatic issues, psychosocial issues, loss of independence and role adjustment. As a result of relocation, it is evident that the impact is profound in terms of an individuals’ mental, emotional, physical and spiritual well-being. Ensuring that adequate social support and education are available to patients and families would aid in alleviating stressors associated with managing chronic kidney disease.
Two-Year Follow-Up of 4-mm-Long Implants Used as Distal Support of Full-Arch FDPs Compared to 10-mm Implants Installed after Sinus Floor Elevation. A Randomized Clinical Trial
Authors: Rossi Fabio , Tuci Lorenzo , Ferraioli Lorenzo , Ricci Emanuele , Suerica Andreea , Botticelli Daniele , Pellegrino Gerardo , Felice Pietro
Published: April 2021

Background: In edentulous patients, bone resorption cannot allow the installation of standard implants and it is demanded to use short implants in the residual alveolar bone or longer implants in grafted bone. Aim: To compare the survival and bone level changes of standard plus short 4-mm implants used as distal support of a maxillary full-arch fixed dental prostheses (FDPs) with standard (10-mm) implants placed in association with a bilateral sinus floor augmentation procedure. Material and Methods: Full-arch FDPs supported by six implants were randomly placed in both groups. In the control group, all implants were 10 mm long and 4.1 mm in diameter. The distal implant in both sides of the maxilla was installed after 4 months from bilaterally sinus floor elevation. In the test group (short group), the distal implant in both sides of the maxilla was 4 mm long and 4.1 mm in diameter. No sinus floor elevations were performed in the test group. Clinical assessments and X-rays were taken at prosthesis delivering and after 6, 12, 18, and 24 months. Patient-reported outcome measures (PROMs) were also evaluated before surgery and after 6, 12, and 24 months. Results: The changes over time of the bone level for the short implants were −0.01 ± 0.11 mm, −0.04 ± 0.13 mm, −0.17 ± 0.29 mm, and −0.28 ± 0.37 mm after 6, 12, 18, and 24 months from prosthesis delivering, respectively. For the standard implants, bone changes were −0.21 ± 0.33 mm (p = 0.103), −0.30 ± 0.32 mm (p = 0.023), −0.40 ± 0.37 mm (p = 0.144), and −0.54 ± 0.49 mm (p = 0.128), respectively. A statistically relevant difference was found only at 12 months after loading between the two groups. Conclusions: Similar results on implant survival rate and marginal bone loss were observed for the short and standard implants, placed in association with a bilateral sinus floor augmentation procedure, used as distal support of a maxillary full-arch FDP. A statistically relevant difference was found only at 12 months after loading between the two groups (p = 0.023).
What Happens in the City When Long-Term Urban Expansion and (Un)Sustainable Fringe Development Occur: The Case Study of Rome
Authors: Nickayin Samaneh Sadat , Salvati Luca , Coluzzi Rosa , Lanfredi Maria , Halbac-Cotoara-Zamfir Rares , Salvia Rosanna , Quaranta Giovanni , Alhuseen Ahmed , Gaburova Luisa
Published: April 2021

This study investigates long-term landscape transformations (1949–2016) in urban Rome, Central Italy, through a spatial distribution of seven metrics (core, islet, perforation, edge, loop, bridge, branch) derived from a Morphological Spatial Pattern Analysis (MSPA) analyzed separately for seven land-use classes (built-up areas, arable land, crop mosaic, vineyards, olive groves, forests, pastures). A Principal Component Analysis (PCA) has been finally adopted to characterize landscape structure at 1949 and 2016. Results of the MSPA demonstrate how both natural and agricultural land-uses have decreased following urban expansion. Moreover, the percent ‘core’ area of each class declined substantially, although with different intensity. These results clearly indicate ‘winners’ and ‘losers’ after long-term landscape transformations: urban settlements and forests belong to the former category, the remaining land-use classes (mostly agricultural) belong to the latter category. Descriptive statistics and multivariate exploratory techniques finally documented the intrinsic complexity characteristic of actual landscapes. The findings of this study also demonstrate how settlements have expanded chaotically over the study area, reflecting a progressive ‘fractalization’ and inhomogeneity of fringe landscapes, with negative implications for metropolitan sustainability at large. These transformations were unable to leverage processes of settlement and economic re-agglomeration around sub-centers typical of polycentric development in the most advanced socioeconomic contexts.
Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation
Authors: Takahashi Shinya , Ferdousi Farhana , Zheng Yun-Wen , Oda Tatsuya , Isoda Hiroko
Published: April 2021

Cyanidin, a kind of anthocyanin, has been reported to have chemotherapeutic activities in humans. Human amniotic epithelial cells (hAECs) are considered a potential source of pluripotent stem cells. hAECs have been used as a novel tool in regenerative cellular therapy and cell differentiation studies. In this study, to explore the effects of cyanidin-3-O-glucoside (Cy3G) on hAECs and their mechanisms, we investigated the transcriptomic changes in the Cy3G-treated cells using microarray analysis. Among the differentially expressed genes (Fold change > 1.1; p-value < 0.05), 109 genes were upregulated and 232 were downregulated. Ratios of upregulated and downregulated genes were 0.22% and 0.47% of the total expressed genes, respectively. Next, we explored the enriched gene ontology, i.e., the biological process, molecular function, and cellular component of the 37 upregulated (>1.3-fold change) and 124 downregulated (<1.3-fold change) genes. Significantly enriched biological processes by the upregulated genes included “response to muscle activity,” and the genes involved in this gene ontology (GO) were Metrnl and SRD5A1, which function in the adipocyte. On the other hand, the cell cycle biological process was significantly enriched by the downregulated genes, including some from the SMC gene family. An adipogenesis-associated gene DDX6 was also included in the cell cycle biological process. Thus, our findings suggest the prospects of Cy3G in modulating adipocyte differentiation in hAECs.
Identification of Genes Involved in Fe–S Cluster Biosynthesis of Nitrogenase in Paenibacillus polymyxa WLY78
Authors: Li Qin , Li Yongbing , Li Xiaohan , Chen Sanfeng
Published: April 2021

NifS and NifU (encoded by nifS and nifU) are generally dedicated to biogenesis of the nitrogenase Fe–S cluster in diazotrophs. However, nifS and nifU are not found in N2-fixing Paenibacillus strains, and the mechanisms involved in Fe–S cluster biosynthesis of nitrogenase is not clear. Here, we found that the genome of Paenibacillus polymyxa WLY78 contains the complete sufCDSUB operon, a partial sufC2D2B2 operon, a nifS-like gene, two nifU-like genes (nfuA-like and yutI), and two iscS genes. Deletion and complementation studies showed that the sufC, sufD, and sufB genes of the sufCDSUB operon, and nifS-like and yutI genes were involved in the Fe–S cluster biosynthesis of nitrogenase. Heterologous complementation studies demonstrated that the nifS-like gene of P. polymyxa WLY78 is interchangeable with Klebsiella oxytoca nifS, but P. polymyxa WLY78 SufCDB cannot be functionally replaced by K. oxytoca NifU. In addition, K. oxytoca nifU and Escherichia coli nfuA are able to complement the P. polymyxa WLY78 yutI mutant. Our findings thus indicate that the NifS-like and SufCDB proteins are the specific sulfur donor and the molecular scaffold, respectively, for the Fe–S cluster formation of nitrogenase in P. polymyxa WLY78. YutI can be an Fe–S cluster carrier involved in nitrogenase maturation in P. polymyxa WLY78.
Corticosteroids for COVID-19 Therapy: Potential Implications on Tuberculosis
Authors: Gopalaswamy Radha , Subbian Selvakumar
Published: April 2021

On 11 March 2020, the World Health Organization announced the Corona Virus Disease-2019 (COVID-19) as a global pandemic, which originated in China. At the host level, COVID-19, caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), affects the respiratory system, with the clinical symptoms ranging from mild to severe or critical illness that often requires hospitalization and oxygen support. There is no specific therapy for COVID-19, as is the case for any common viral disease except drugs to reduce the viral load and alleviate the inflammatory symptoms. Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis (Mtb), also primarily affects the lungs and has clinical signs similar to pulmonary SARS-CoV-2 infection. Active TB is a leading killer among infectious diseases and adds to the burden of the COVID-19 pandemic worldwide. In immunocompetent individuals, primary Mtb infection can also lead to a non-progressive, asymptomatic latency. However, latent Mtb infection (LTBI) can reactivate symptomatic TB disease upon host immune-suppressing conditions. Importantly, the diagnosis and treatment of TB are hampered and admixed with COVID-19 control measures. The US-Center for Disease Control (US-CDC) recommends using antiviral drugs, Remdesivir or corticosteroid (CST), such as dexamethasone either alone or in-combination with specific recommendations for COVID-19 patients requiring hospitalization or oxygen support. However, CSTs can cause immunosuppression, besides their anti-inflammatory properties. The altered host immunity during COVID-19, combined with CST therapy, poses a significant risk for new secondary infections and/or reactivation of existing quiescent infections, such as LTBI. This review highlights CST therapy recommendations for COVID-19, various types and mechanisms of action of CSTs, the deadly combination of two respiratory infectious diseases COVID-19 and TB. It also discusses the importance of screening for LTBI to prevent TB reactivation during corticosteroid therapy for COVID-19.
Study on Demethoxycurcumin as a Promising Approach to Reverse Methicillin-Resistance of Staphylococcus aureus
Authors: Li Qian-Qian , Kang Ok-Hwa , Kwon Dong-Yeul
Published: April 2021

Methicillin-resistant Staphylococcus aureus (MRSA) has always been a threatening pathogen. Research on phytochemical components that can replace antibiotics with limited efficacy may be an innovative method to solve intractable MRSA infections. The present study was devoted to investigate the antibacterial activity of the natural compound demethoxycurcumin (DMC) against MRSA and explore its possible mechanism for eliminating MRSA. The minimum inhibitory concentrations (MICs) of DMC against MRSA strains was determined by the broth microdilution method, and the results showed that the MIC of DMC was 62.5 μg/mL. The synergistic effects of DMC and antibiotics were investigated by the checkerboard method and the time–kill assay. The ATP synthase inhibitors were employed to block the metabolic ability of bacteria to explore their synergistic effect on the antibacterial ability of DMC. In addition, western blot analysis and qRT-PCR were performed to detect the proteins and genes related to drug resistance and S. aureus exotoxins. As results, DMC hindered the translation of penicillin-binding protein 2a (PBP2a) and staphylococcal enterotoxin and reduced the transcription of related genes. This study provides experimental evidences that DMC has the potential to be a candidate substance for the treatment of MRSA infections.
The Fibronectin Expression Determines the Distinct Progressions of Malignant Gliomas via Transforming Growth Factor-Beta Pathway
Authors: Chen Chih-Wei , Yang Cheng-Han , Lin Yuan-Ho , Hou Ya-Chin , Cheng Tain-Junn , Chang Sheng-Tsung , Huang Yu-Hua , Chung Shang-Ting , Chio Chung-Ching , Shan Yan-Shen , Cheng Hung-Chi , Chang Wen-Tsan
Published: April 2021

Due to the increasing incidence of malignant gliomas, particularly glioblastoma multiforme (GBM), a simple and reliable GBM diagnosis is needed to screen early the death-threaten patients. This study aimed to identify a protein that can be used to discriminate GBM from low-grade astrocytoma and elucidate further that it has a functional role during malignant glioma progressions. To identify proteins that display low or no expression in low-grade astrocytoma but elevated levels in GBM, glycoprotein fibronectin (FN) was particularly examined according to the mining of the Human Protein Atlas. Web-based open megadata minings revealed that FN was mainly mutated in the cBio Cancer Genomic Portal but dominantly overexpressed in the ONCOMINE (a cancer microarray database and integrated data-mining platform) in distinct tumor types. Furthermore, numerous different cancer patients with high FN indeed exhibited a poor prognosis in the PrognoScan mining, indicating that FN involves in tumor malignancy. To investigate further the significance of FN expression in glioma progression, tumor specimens from five malignant gliomas with recurrences that received at least two surgeries were enrolled and examined. The immunohistochemical staining showed that FN expression indeed determined the distinct progressions of malignant gliomas. Furthermore, the expression of vimentin (VIM), a mesenchymal protein that is strongly expressed in malignant cancers, was similar to the FN pattern. Moreover, the level of epithelial–mesenchymal transition (EMT) inducer transforming growth factor-beta (TGF-β) was almost recapitulated with the FN expression. Together, this study identifies a protein FN that can be used to diagnose GBM from low-grade astrocytoma; moreover, its expression functionally determines the malignant glioma progressions via TGF-β-induced EMT pathway.
Novel and Potent Small Molecules against Melanoma Harboring BRAF Class I/II/III Mutants for Overcoming Drug Resistance
Authors: Kim Namkyoung , Shin Injae , Lee Jiwon , Jeon Eunhye , Kim Younghoon , Ryu Seongshick , Ju Eunhye , Cho Wonjeong , Sim Taebo
Published: April 2021

Melanoma accounts for the majority of skin cancer deaths. About 50% of all melanomas are associated with BRAF mutations. BRAF mutations are classified into three classes with regard to dependency on RAF dimerization and RAS signaling. The most frequently occurring class I BRAF V600 mutations are sensitive to vemurafenib whereas class II and class III mutants, non-V600 BRAF mutants are resistant to vemurafenib. Herein we report six pyrimido[4,5-d]pyrimidin-2-one derivatives possessing highly potent anti-proliferative activities on melanoma cells harboring BRAF class I/II/III mutants. Novel and most potent derivative, SIJ1777, possesses not only two-digit nanomolar potency but also 2 to 14-fold enhanced anti-proliferative activities compared with reference compound, GNF-7 against melanoma cells (SK-MEL-2, SK-MEL-28, A375, WM3670, WM3629). Moreover, SIJ1777 substantially inhibits the activation of MEK, ERK, and AKT and remarkably induces apoptosis and significantly blocks migration, invasion, and anchorage-independent growth of melanoma cells harboring BRAF class I/II/II mutations while both vemurafenib and PLX8394 have little to no effects on melanoma cells expressing BRAF class II/III mutations. Taken together, our six GNF-7 derivatives exhibit highly potent activities against melanoma cells harboring class I/II/III BRAF mutations compared with vemurafenib as well as PLX8394.
Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation
Authors: Ibrahim Hussam , Reus Philipp , Mundorf Anna Katharina , Grothoff Anna-Lena , Rudenko Valerie , Buschhaus Christina , Stefanski Anja , Berleth Niklas , Stork Björn , Stühler Kai , Kalfalah Faiza , Reinke Hans
Published: April 2021

Repressor protein period (PER) complexes play a central role in the molecular oscillator mechanism of the mammalian circadian clock. While the main role of nuclear PER complexes is transcriptional repression, much less is known about the functions of cytoplasmic PER complexes. We found with a biochemical screen for PER2-interacting proteins that the small GTPase regulator GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1), which has been identified previously as a component of cytoplasmic PER complexes in mice, is also a bona fide component of human PER complexes. We show that in situ GAPVD1 is closely associated with casein kinase 1 delta (CSNK1D), a kinase that regulates PER2 levels through a phosphoswitch mechanism, and that CSNK1D regulates the phosphorylation of GAPVD1. Moreover, phosphorylation determines the kinetics of GAPVD1 degradation and is controlled by PER2 and a C-terminal autoinhibitory domain in CSNK1D, indicating that the regulation of GAPVD1 phosphorylation is a novel function of cytoplasmic PER complexes and might be part of the oscillator mechanism or an output function of the circadian clock.
Recent Perspectives on Sex Differences in Compulsion-Like and Binge Alcohol Drinking
Authors: Radke Anna K. , Sneddon Elizabeth A. , Frasier Raizel M. , Hopf Frederic W.
Published: April 2021

Alcohol use disorder remains a substantial social, health, and economic problem and problem drinking levels in women have been increasing in recent years. Understanding whether and how the underlying mechanisms that drive drinking vary by sex is critical and could provide novel, more targeted therapeutic treatments. Here, we examine recent results from our laboratories and others which we believe provide useful insights into similarities and differences in alcohol drinking patterns across the sexes. Findings for binge intake and aversion-resistant, compulsion-like alcohol drinking are considered, since both are likely significant contributors to alcohol problems in humans. We also describe studies regarding mechanisms that may underlie sex differences in maladaptive alcohol drinking, with some focus on the importance of nucleus accumbens (NAcb) core and shell regions, several receptor types (dopamine, orexin, AMPA-type glutamate), and possible contributions of sex hormones. Finally, we discuss how stressors such as early life stress and anxiety-like states may interact with sex differences to contribute to alcohol drinking. Together, these findings underscore the importance and critical relevance of studying female and male mechanisms for alcohol and co-morbid conditions to gain a true and clinically useful understanding of addiction and neuropsychiatric mechanisms and treatment.
Argon Plasma Exposure Augments Costimulatory Ligands and Cytokine Release in Human Monocyte-Derived Dendritic Cells
Authors: Bekeschus Sander , Meyer Dorothee , Arlt Kevin , von Woedtke Thomas , Miebach Lea , Freund Eric , Clemen Ramona
Published: April 2021

Cold physical plasma is a partially ionized gas expelling many reactive oxygen and nitrogen species (ROS/RNS). Several plasma devices have been licensed for medical use in dermatology, and recent experimental studies suggest their putative role in cancer treatment. In cancer therapies with an immunological dimension, successful antigen presentation and inflammation modulation is a key hallmark to elicit antitumor immunity. Dendritic cells (DCs) are critical for this task. However, the inflammatory consequences of DCs following plasma exposure are unknown. To this end, human monocyte-derived DCs (moDCs) were expanded from isolated human primary monocytes; exposed to plasma; and their metabolic activity, surface marker expression, and cytokine profiles were analyzed. As controls, hydrogen peroxide, hypochlorous acid, and peroxynitrite were used. Among all types of ROS/RNS-mediated treatments, plasma exposure exerted the most notable increase of activation markers at 24 h such as CD25, CD40, and CD83 known to be crucial for T cell costimulation. Moreover, the treatments increased interleukin (IL)-1α, IL-6, and IL-23. Altogether, this study suggests plasma treatment augmenting costimulatory ligand and cytokine expression in human moDCs, which might exert beneficial effects in the tumor microenvironment.
Predicting the Structure and Dynamics of Membrane Protein GerAB from Bacillus subtilis
Authors: Blinker Sophie , Vreede Jocelyne , Setlow Peter , Brul Stanley
Published: April 2021

Bacillus subtilis forms dormant spores upon nutrient depletion. Germinant receptors (GRs) in spore’s inner membrane respond to ligands such as L-alanine, and trigger spore germination. In B. subtilis spores, GerA is the major GR, and has three subunits, GerAA, GerAB, and GerAC. L-Alanine activation of GerA requires all three subunits, but which binds L-alanine is unknown. To date, how GRs trigger germination is unknown, in particular due to lack of detailed structural information about B subunits. Using homology modelling with molecular dynamics (MD) simulations, we present structural predictions for the integral membrane protein GerAB. These predictions indicate that GerAB is an α-helical transmembrane protein containing a water channel. The MD simulations with free L-alanine show that alanine binds transiently to specific sites on GerAB. These results provide a starting point for unraveling the mechanism of L-alanine mediated signaling by GerAB, which may facilitate early events in spore germination.
Assessment of the Seismic Response of CLT Shear Walls Using the EEGBW, a Bouc–Wen Class Predictive Model
Authors: Aloisio Angelo , Fragiacomo Massimo
Published: April 2021

The paper presents an application of the Extended Energy-dependent Generalized Bouc–Wen model (EEGBW) to simulate the experimental cyclic response of Cross-Laminated Timber (CLT) panels. The main objectives of the paper are assessing the sensitivity of the quadratic error between experimental and numerical data to the EEGBW parameters, showing the fitting performance of the EEGBW model in matching the experimental cyclic response of CLT panels, highlighting the stability of the model in nonlinear dynamic analysis with seismic excitation. The research proves that the considered Bouc–Wen class hysteresis model can reproduce the hysteretic response of structural arrangements characterized by pinching and degradation phenomena. The model exhibits significant stability in nonlinear dynamic analysis with seismic excitation. The model’s stability and versatility endorse its application to simulate structural systems’ dynamic response when Finite Element modelling might be an impractical choice.
Insect Decline—A Forensic Issue?
Authors: Amendt Jens
Published: April 2021

Recent reports have shown a dramatic loss in insect species and biomass. Since forensic entomology relies on the presence of insects, the question is whether this decline effects the discipline. The present review confirms that numerous studies document insect population declines or even extinction, despite the fact that the rates of decline and the methods used to demonstrate it are still much debated. However, with regard to a decline in necrophagous insects, there is little or only anecdotal data available. A hypothetical decrease in species diversity and population density in necrophagous insects could lead to a delayed colonization of dead bodies and a modified succession pattern due to the disappearance or new occurrence of species or their altered seasonality. Climate change as one of the drivers of insect decline will probably also have an impact on necrophagous insects and forensic entomology, leading to reduced flight and oviposition activity, modified growth rates and, therefore, an over- or underestimation of a minimum postmortem interval. Global warming with increased temperature and extreme weather requires a better understanding about necrophagous insect responses to environmental variations. Here, transgeneration effects in particular should be analysed in greater depth as this will help to understand rapid adaptation and plasticity in insects of forensic importance.
The Emerging Role of Robotics in Pelvic Exenteration Surgery for Locally Advanced Rectal Cancer: A Narrative Review
Authors: Chang Tou Pin , Chok Aik Yong , Tan Dominic , Rogers Ailin , Rasheed Shahnawaz , Tekkis Paris , Kontovounisios Christos
Published: April 2021

Pelvic exenteration surgery for locally advanced rectal cancers is a complex and extensive multivisceral operation, which is associated with high perioperative morbidity and mortality rates. Significant technical challenges may arise due to inadequate access, visualisation, and characterisation of tissue planes and critical structures in the spatially constrained pelvis. Over the last two decades, robotic-assisted technologies have facilitated substantial advancements in the minimally invasive approach to total mesorectal excision (TME) for rectal cancers. Here, we review the emerging experience and evidence of robotic assistance in beyond TME multivisceral pelvic exenteration for locally advanced rectal cancers where heightened operative challenges and cumbersome ergonomics are likely to be encountered.
Trends in Hospital Admissions for Chronic Obstructive Pulmonary Disease in Men and Women in Spain, 1998 to 2018
Authors: Orozco-Beltrán Domingo , Arriero-Marin Juan Manuel , Carratalá-Munuera Concepción , Soler-Cataluña Juan J. , Lopez-Pineda Adriana , Gil-Guillén Vicente F. , Quesada Jose A.
Published: April 2021

The prevalence of chronic obstructive pulmonary disease (COPD) is rising faster in women in some countries. An observational time trends study was performed to assess the evolution of hospital admissions for COPD in men and women in Spain from 1998 to 2018. ICD-9 diagnostic codes (490–492, 496) from the minimum basic data set of hospital discharges were used. Age-standardised admission rates were calculated using the European Standard Population. Joinpoint regression models were fitted to estimate the annual percent change (APC). In 2018, the age-standardised admission rate per 100,000 population/year for COPD was five times higher in men (384.8, 95% CI: 381.7, 387.9) than in women (78.6, 95% CI: 77.4, 79.9). The average annual percent change (AAPC) was negative over the whole study period in men (−1.7%/year, 95% CI: −3.1, −0.2) but positive from 2010 to 2018 (1.1%/year, 95% CI: −0.8, 2.9). In women, the APC was −6.0% (95%CI: −7.1, −4.9) from 1998 to 2010, but the trend reversed direction in the 2010–2018 period (7.8%/year, 95% CI: 5.5, 10.2). Thus, admission rates for COPD decreased from 1998 to 2010 in both men and women but started rising again until 2018, modestly in men and sharply in women.
Brain Glucose Metabolism in Health, Obesity, and Cognitive Decline—Does Insulin Have Anything to Do with It? A Narrative Review
Authors: Rebelos Eleni , Rinne Juha O. , Nuutila Pirjo , Ekblad Laura L.
Published: April 2021

Imaging brain glucose metabolism with fluorine-labelled fluorodeoxyglucose ([18F]-FDG) positron emission tomography (PET) has long been utilized to aid the diagnosis of memory disorders, in particular in differentiating Alzheimer’s disease (AD) from other neurological conditions causing cognitive decline. The interest for studying brain glucose metabolism in the context of metabolic disorders has arisen more recently. Obesity and type 2 diabetes—two diseases characterized by systemic insulin resistance—are associated with an increased risk for AD. Along with the well-defined patterns of fasting [18F]-FDG-PET changes that occur in AD, recent evidence has shown alterations in fasting and insulin-stimulated brain glucose metabolism also in obesity and systemic insulin resistance. Thus, it is important to clarify whether changes in brain glucose metabolism are just an epiphenomenon of the pathophysiology of the metabolic and neurologic disorders, or a crucial determinant of their pathophysiologic cascade. In this review, we discuss the current knowledge regarding alterations in brain glucose metabolism, studied with [18F]-FDG-PET from metabolic disorders to AD, with a special focus on how manipulation of insulin levels affects brain glucose metabolism in health and in systemic insulin resistance. A better understanding of alterations in brain glucose metabolism in health, obesity, and neurodegeneration, and the relationships between insulin resistance and central nervous system glucose metabolism may be an important step for the battle against metabolic and cognitive disorders.