Activity against SARS-CoV-2 of Various Anionic Disinfectants and Their Complexes with Hydrophobically Modified Chitosan
Authors:   Department of Physics, Lomonosov Moscow State University, Moscow, Russia, Ospennikov Alexander S. , Kornilaeva Galina V. ,   Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, Moscow, Russia, Larichev Viktor F. ,   Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, Moscow, Russia, Fedyakina Irina T. ,   Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, Moscow, Russia, Fu Lifeng ,   CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, China, Chen Zhuo ,   Shanghai Key Laboratory of Chemical Biology, Shanghai key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China, Yang Yangyang ,   Shanghai Key Laboratory of Chemical Biology, Shanghai key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, China, Shibaev Andrey V. ,   Department of Physics, Lomonosov Moscow State University, Moscow, Russia,   Karagandy University of the name of academician E.A. Buketov, Karaganda, Kazakhstan, Karamov Eduard V. ,   Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, Moscow, Russia,   National Medical Research Center of Phthisiopulmonology and Infectious Diseases of the Russian Ministry of Health, Moscow, Russia, Turgiev Ali S. ,   Gamaleya National Research Center for Epidemiology and Microbiology of the Russian Ministry of Health, Moscow, Russia,   National Medical Research Center of Phthisiopulmonology and Infectious Diseases of the Russian Ministry of Health, Moscow, Russia, Duan Liping ,   NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China, Liu William J. ,   8NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, China, Philippova Olga E. ,   Department of Physics, Lomonosov Moscow State University, Moscow, Russia
Published: June 2023

The aim of this work was to study virucidal activity against SARS-CoV-2 of several anionic disinfectants, which are antiseptics recommended by the U.S. Environmental Protection Agency (EPA), and to prepare their complexes with hydrophobically modified chitosan active against SARS-CoV-2. Experiments were per-formed using a clinical isolate of SARS-CoV-2 obtained from a patient in 2020. It was shown that sodium dodecyl benzene sulfonate (SDBS) is already active at rather small concentrations (above 2 mM), which completely deactivate the virus. In the same concentration range, sodium caprylate does not show activity; and sodium lactate is active against SARS-CoV-2 only at much higher concentrations (225 mM). The most effective disinfectant — sodium dodecylbenzene sulfonate — was used to prepare complexes with hydrophobically modified chitosan. It was found that such complexes exhibit antiviral activity at very low concentrations (1.9 mM chitosan monomer units and 0.25 mM SDBS), at which the polymer without surfac-tant is not active against SARS-CoV-2.