bluuebunny/crossref_metadata_2025 huggingface dataset:
“I don’t Think These Devices are Very Culturally Sensitive.”—Impact of Automated Speech Recognition Errors on African Americans
Authors: Mengesha Zion , Heldreth Courtney , Lahav Michal , Sublewski Juliana , Tuennerman Elyse
Published: December 2021

Automated speech recognition (ASR) converts language into text and is used across a variety of applications to assist us in everyday life, from powering virtual assistants, natural language conversations, to enabling dictation services. While recent work suggests that there are racial disparities in the performance of ASR systems for speakers of African American Vernacular English, little is known about the psychological and experiential effects of these failures paper provides a detailed examination of the behavioral and psychological consequences of ASR voice errors and the difficulty African American users have with getting their intents recognized. The results demonstrate that ASR failures have a negative, detrimental impact on African American users. Specifically, African Americans feel othered when using technology powered by ASR—errors surface thoughts about identity, namely about race and geographic location—leaving them feeling that the technology was not made for them. As a result, African Americans accommodate their speech to have better success with the technology. We incorporate the insights and lessons learned from sociolinguistics in our suggestions for linguistically responsive ways to build more inclusive voice systems that consider African American users’ needs, attitudes, and speech patterns. Our findings suggest that the use of a diary study can enable researchers to best understand the experiences and needs of communities who are often misunderstood by ASR. We argue this methodological framework could enable researchers who are concerned with fairness in AI to better capture the needs of all speakers who are traditionally misheard by voice-activated, artificially intelligent (voice-AI) digital systems.
Adaptive Initialization Method for K-Means Algorithm
Authors: Yang Jie , Wang Yu-Kai , Yao Xin , Lin Chin-Teng
Published: December 2021

The K-means algorithm is a widely used clustering algorithm that offers simplicity and efficiency. However, the traditional K-means algorithm uses a random method to determine the initial cluster centers, which make clustering results prone to local optima and then result in worse clustering performance. In this research, we propose an adaptive initialization method for the K-means algorithm (AIMK) which can adapt to the various characteristics in different datasets and obtain better clustering performance with stable results. For larger or higher-dimensional datasets, we even leverage random sampling in AIMK (name as AIMK-RS) to reduce the time complexity. 22 real-world datasets were applied for performance comparisons. The experimental results show AIMK and AIMK-RS outperform the current initialization methods and several well-known clustering algorithms. Specifically, AIMK-RS can significantly reduce the time complexity to O (n). Moreover, we exploit AIMK to initialize K-medoids and spectral clustering, and better performance is also explored. The above results demonstrate superior performance and good scalability by AIMK or AIMK-RS. In the future, we would like to apply AIMK to more partition-based clustering algorithms to solve real-life practical problems.
A Value-Based Steering Model for Healthcare
Authors: Pitkänen Laura J. , Leskelä Riikka-Leena , Tolkki Helena , Torkki Paulus
Published: December 2021

This article aims to answer how a commissioning body can steer health services based on value in an environment where the commissioner is responsible for the health services of a population with varying health service needs. In this design science study, we constructed a value-based steering model consisting of three parts: (1) the principles of steering; (2) the steering process; and (3) Value Steering Canvas, a concrete tool for steering. The study is based on Finland, a tax-funded healthcare system, where healthcare is a public service. The results can be applied in any system where there is a commissioner and a service provider, whether they are two separate organizations or not. We conclude that steering can be done based on value. The commissioning body can start using value-based steering without changes in legislation or in the present service system. Further research is needed to test the model in practice.
Planning Fail-Safe Trajectories for Space Robotic Arms
Authors: Porges Oliver , Leidner Daniel , Roa Máximo A.
Published: December 2021

A frequent concern for robot manipulators deployed in dangerous and hazardous environments for humans is the reliability of task executions in the event of a joint failure. A redundant robotic manipulator can be used to mitigate the risk and guarantee a post-failure task completion, which is critical for instance for space applications. This paper describes methods to analyze potential risks due to a joint failure, and introduces tools for fault-tolerant task design and path planning for robotic manipulators. The presented methods are based on off-line precomputed workspace models. The methods are general enough to cope with robots with any type of joint (revolute or prismatic) and any number of degrees of freedom, and might include arbitrarily shaped obstacles in the process, without resorting to simplified models. Application examples illustrate the potential of the approach.
From Tobacco Cigarettes to Electronic Cigarettes: The Two Sides of a Nicotine Coin
Authors: Besaratinia Ahmad
Published: December 2021

Tobacco smoking-related diseases, including cardiovascular disease, pulmonary disease, stroke, and cancer in multiple organ sites, are the leading causes of preventable death, worldwide. Youth electronic cigarette use (vaping) is an evolving public health problem in the United States and around the world. Many of the same toxicants and carcinogens present in tobacco smoke are also found in electronic cigarette vapor, although mostly at substantially lower levels. The reduced concentrations of these chemicals in electronic cigarette vapor may imply lower health risk; however, they cannot equate to no risk. To date, the long-term health consequences of vaping are largely unknown. This “Perspective” provides a concise chronology of events leading up to an unprecedented global challenge, namely the convergence of global tobacco epidemic and youth vaping epidemic. Current state of knowledge, outstanding questions in the field, present challenges, and future directions in research are highlighted. The existing data show a continued and dynamic evolution of the converged epidemics. The goal should be to prevent youth vaping while improving smoking cessation strategies. In smokers who are unable or unwilling to quit smoking, the objective should be to provide “provably” safe or less-harmful alternatives, which should “completely” or “substantially” substitute tobacco cigarettes.
Efficacy of a Customized Three-Dimensional Printing Surgical Guide for Tibial Plateau Leveling Osteotomy: A Comparison With Conventional Tibial Plateau Leveling Osteotomy
Authors: Kim Jayon , Ko Jaeeun , Kim Jaehwan , Seo Anna , Eom Kidong
Published: December 2021

Objective: To prospectively evaluate the effect of a computed tomography (CT)-based three-dimensional (3D) printing surgical guide on surgical accuracy of tibial plateau leveling osteotomy (TPLO).

Study Design: Cadaveric study.

Animals: Canine cadaveric hindlimbs (n = 14).

Methods: TPLO was performed on cadaver hindlimbs disarticulated at the coxofemoral joint to compare and evaluate the conventional TPLO method (n = 7) with one that used customized 3D printing surgical guides (n = 7). The operation time and postoperative tibial plateau angle (TPA) of the osteotomy were evaluated. Moreover, the osteotomy inclination, torsion, and distance and the direction of eccentricity were assessed using CT reconstruction.

Results: Significant differences in the operation time (p < 0.001), postoperative TPA (p < 0.05), osteotomy inclination (p < 0.05), and osteotomy torsion (p < 0.05) were observed.

Conclusion: The use of TPLO surgical guide reduced the operation time and inaccurate osteotomy.

Clinical Significance: The surgical technique applied with a customized 3D printing surgical guide could be used to perform osteotomy and TPA adjustment more precisely than conventional TPLO.
Case Report: Treatment of Femoral Non-union With Rib and Iliac Crest Autografts and rhBMP-2 in a Cat
Authors: Chung Cheng-Shu , Lin Lee-Shuan , Teo Yi-Min
Published: December 2021

A 5-year-old, intact male Bengal cat weighing 5.2 kg was referred for the fixation failure of a right femoral fracture. Multiple surgical revisions failed, and atrophic non-union was diagnosed. The cat was then admitted for a final revision surgery using locking plate fixation in conjunction with rib and iliac crest autografts and recombinant human bone morphogenetic protein 2 (rhBMP-2). The fracture site was debrided and stabilized before filling the defect with 1.8 cm of rib bone autograft. The residual space in the defect was then filled with an iliac crest autograft. Finally, a 3 ×5 cm absorbable collagen sponge soaked with 0.5 mL of 0.2 mg/mL rhBMP-2 solution was placed around the defect. No significant complications were noted postoperatively. Bone healing was noted 2 months postoperatively, and it continued for 12 months. Although mild lameness remained, the cat's ambulatory function and quality of life were good. To the authors' knowledge, this is the first case report of a clinical transplantation of a rib segment as an autograft in combination with rhBMP-2 in a cat with a large bone defect.
Common Uses and Adverse Effects of Hyperbaric Oxygen Therapy in a Cohort of Small Animal Patients: A Retrospective Analysis of 2,792 Treatment Sessions
Authors: Montalbano Christina , Kiorpes Caroline , Elam Lindsay , Miscioscia Erin , Shmalberg Justin
Published: December 2021

Hyperbaric oxygen therapy (HBOT) is commonly utilized for various human conditions with a low incidence of major adverse effects (0.002–0.035%). Despite growing use in veterinary patients, there remains a paucity of literature describing its use and associated complications. The purpose of this study was to report clinical use of HBOT in small animals and identify the rate of major adverse events at a university teaching hospital. Electronic medical records were searched for small animals receiving HBOT between November 2012 and February 2020. Data extracted from the medical records included signalment, treatment indication, and adverse events. Treatment sessions totaled 2,792 in 542 dogs, 24 cats, and 10 pocket pets and exotics. Common indications included neurologic injuries (50.4%), tissue healing (31.4%), control of oomycete infection (5.5%), neoplasia or post-radiation injury (5.4%), and various miscellaneous conditions (7.4%). Observed minor adverse events included agitation in two dogs and vomiting in three dogs. The most common major adverse event was central nervous system (CNS) oxygen toxicity in 19 dogs. Central nervous system oxygen toxicity, manifesting as focal or generalized seizures, occurred in 0.7% of treatment sessions, with increasing age (p = 0.01) and female sex (p = 0.01) identified as risk factors. One dog developed pulmonary edema following HBOT which is a reported adverse event in humans or may have been a manifestation of progression of the dog's underlying disease. No adverse events were noted in cats or other species. In conclusion, HBOT appeared safe across various indications, although oxygen toxicity affecting the CNS was higher than reports in humans. Future prospective, randomized, controlled trials should evaluate specific clinical indications and outcomes.
Milk, Fertility and Udder Health Performance of Purebred Holstein and Three-Breed Rotational Crossbred Cows within French Farms: Insights on the Benefits of Functional Diversity
Authors: Quénon Julien , Magne Marie-Angélina
Published: December 2021

Using three-breed rotational crossbreeding in a purebred Holstein (HO) herd raises two questions: Do the different genetic classes of cows generated by crossbreeding perform differently? Are there any economic benefits of combining them within a herd? This study aimed at comparing the performance between the different genetic classes resulting from the use of three-breed rotational crossbreeding, and simulating the effect of combining them on herd profitability. Based on a dataset of 14 French commercial dairy herds using three-bred rotational crossbreeding from a HO herd over a 10-year period, we defined three genetic classes according to the theoretical value of heterosis and the percentage of HO genes. We performed linear models and estimated least square means to compare HO cows and the first and second generation of crosses (F1 and G2, respectively) on eight performance characteristics related to milk yield and solids, udder health and fertility. We used these to simulate profitability of five herd compositions differing according to HO, F1 and G2 proportions. We showed that HO, F1 and G2 cows had different and complementary performance profiles. HO had a win-lost trade-off between milk yield and fertility, G2 had the opposite trade-off and F1 had a win-win trade-off. Differences regarding milk solids and udder health were less clear-cut. We highlighted that combining HO with F1 or with both F1 and G2 (below 30%) could be more profitable than using purebred HO or crossbred herds in a conventional milk price scenario. These findings provide evidence on the benefits of functional diversity generated from the use of dairy crossbreeding in dairy herds.
The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off
Authors: Parés Sílvia , Cano-Garrido Olivia , Bach Alex , Ferrer-Miralles Neus , Villaverde Antonio , Garcia-Fruitós Elena , Arís Anna
Published: December 2021

The dry period is decisive for the milking performance of dairy cows. The promptness of mammary gland involution at dry-off affects not only the productivity in the next lactation, but also the risk of new intra-mammary infections since it is closely related with the activity of the immune system. Matrix metalloproteinase-9 (MMP-9) is an enzyme present in the mammary gland and has an active role during involution by disrupting the extracellular matrix, mediating cell survival and the recruitment of immune cells. The objective of this study was to determine the potential of exogenous administration of a soluble and recombinant version of a truncated MMP-9 (rtMMP-9) to accelerate mammary involution and boost the immune system at dry-off, avoiding the use of antibiotics. Twelve Holstein cows were dried abruptly, and two quarters of each cow received an intra-mammary infusion of either soluble rtMMP-9 or a positive control based on immunostimulant inclusion bodies (IBs). The contralateral quarters were infused with saline solution as negative control. Samples of mammary secretion were collected during the week following dry-off to determine SCC, metalloproteinase activity, bovine serum albumin, lactoferrin, sodium, and potassium concentrations. The soluble form of rtMMP-9 increased endogenous metalloproteinase activity in the mammary gland compared with saline quarters but did not accelerate either the immune response or involution in comparison with control quarters. The results demonstrated that the strategy to increase the mammary gland immunocompetence by recombinant infusion of rtMMP-9 was unsuccessful.
Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park
Authors: Rodionov Andrey , Deniskova Tatiana , Dotsev Arsen , Volkova Valeria , Petrov Sergey , Kharzinova Veronika , Koshkina Olga , Abdelmanova Alexandra , Solovieva Anastasia , Shakhin Alexey , Bardukov Nikolay , Zinovieva Natalia
Published: December 2021

Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.
A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of Takifugu rubripes
Authors: Zhou He , Sun Yuqing , Li Xin , Zhou Ziyu , Ma Kexin , Guo Wenxuan , Liang Yuting , Xie Xingyi , Zhang Jingxian , Wang Qian , Liu Yang
Published: December 2021

The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of Takifugu rubripes, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of T. rubripes (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (LOC101066759, dgat1, limk1, fbxl3, col6a3, fgfr3, dusp22b, svil, abhd17b, srgap3, tmem88b, bud4, and mustn10) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for T. rubripes and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.
Effects of Multimodal Analgesic Protocol, with Buprenorphine and Meloxicam, on Mice Well-Being: A Dose Finding Study
Authors: Furumoto Kayo , Ogita Kumi , Kamisaka Tomomi , Kawasumi Asami , Takata Koushi , Maeta Noritaka , Itoi Takamasa , Nohara Masakatsu , Saeki Kaori , Kanda Teppei
Published: December 2021

The anesthetic or analgesic agent of choice, route and frequency of anesthetic or analgesic administration, and stressors induce distress during the perioperative period. We evaluated a multimodal analgesic protocol using buprenorphine and meloxicam on the well-being of mice. Twenty-four Slc:ICR male mice were divided into control, anesthesia + analgesia, and surgery + anesthesia + analgesia groups. Tap water (orally: PO) and water for injection (subcutaneous: SC) were administered to the control group. Buprenorphine was administered twice (SC, 0.1 mg/kg/8 h) and meloxicam was administered thrice (PO, 5 mg/kg/24 h) to the anesthesia + analgesia and surgery + anesthesia + analgesia groups. The mice were subjected to laparotomy and assessed for several parameters. Even in absence of surgical pain, the anesthesia + analgesia group presented the same negative effects as the surgery + anesthesia + analgesia group. This multimodal analgesic protocol for mice was expected to have an analgesic effect on pain associated with laparotomy but was not sufficient to prevent food intake and weight decrease. This does not negate the need to administer analgesics, but suggests the need to focus on and care not only about the approach to relieve pain associated with surgery, but also other types of distresses to minimize negative side effects that may interfere with postoperative recovery in mice.
The Role of Landscape Design in Cultural Rural Areas. A Didactic Exercise to Experiment a Research-by-Design Process Applied to an Italian UNESCO Wine Site
Authors: Dezio Catherine , Zhang Can , Zhang Yilan , Marino Davide
Published: December 2021

Rural landscapes all over the world are subject to great transformations, first being the continuous and slow depopulation of towns and villages. It is a dramatic phenomenon that causes devastating consequences for environmental systems and for the tangible and intangible heritage of entire territories. The situation becomes more ambiguous when it comes to cultural landscapes, especially those internationally recognized as exceptional (i.e., inscribed on the UNESCO (The United Nations Educational, Scientific and Cultural Organization) World Heritage List). In this case, the risk is to abandon agricultural production in favor of consumerist tourist economies, which can damage the territorial authenticity. In this paper, we question the role of the landscape design in strengthening territorial resilience. In particular, a composite and interdependent action has been proposed between landscape design and implementation of a multifunctional agriculture model, oriented towards tourism. To undertake this investigation, a master’s thesis work on Landscape Architecture has been examined as an opportunity to test the research-by-design method through the didactic process. The application case is the Italian UNESCO site of Vignale Monferrato, a depopulated rural village, characterized by abandoned land and buildings. The paper concludes by outlining replicability application scenarios for the proposed model.
Performance Evaluation: Identifying Barriers and Enablers for Landscape Architecture Practice
Authors: Chen Guanyu , Bowring Jacky , Davis Shannon
Published: December 2021

Performance evaluation is crucial for environmental design and sustainable development, especially so for architecture and landscape architecture. However, such performance evaluations remain rare in practice. It is argued that the concerns over potential negative evaluations and a lack of funding are the two main barriers preventing the undertaking of performance evaluations. This research investigated how these two barriers were overcome in practice by studying 41 evaluation cases in the New Zealand landscape architecture field, as well as several international and architectural case studies for comparison. A range of enablers for performance evaluation practices were identified by this research, including funding sources and models that were not documented by existing literature, as well as two strategies for handling the risks of negative evaluation. All of the identified enablers share the same underlying logic—the benefits and costs of an evaluation should be well-regulated by certain mechanisms to keep the benefits of an evaluation greater than, or at least balanced with, the costs, for all the parties involved in the evaluation.
Evapotranspiration Estimation Using Remote Sensing Technology Based on a SEBAL Model in the Upper Reaches of the Huaihe River Basin
Authors: Tan Linshan , Zheng Kaiyuan , Zhao Qiangqiang , Wu Yanjuan
Published: December 2021

Understanding the spatial and temporal variations of evapotranspiration (ET) is vital for water resources planning and management and drought monitoring. The development of a satellite remote sensing technique is described to provide insight into the estimation of ET at a regional scale. In this study, the Surface Energy Balance Algorithm for Land (SEBAL) was used to calculate the actual ET on a daily scale from Landsat-8 data and daily ground-based meteorological data in the upper reaches of Huaihe River on 20 November 2013, 16 April 2015 and 23 March 2018. In order to evaluate the performance of the SEBAL model, the daily SEBAL ET (ETSEBAL) was compared against the daily reference ET (ET0) from four theoretical methods: the Penman-Monteith (P-M), Irmak-Allen (I-A), the Turc, and Jensen-Haise (J-H) method, the ETMOD16 product from the MODerate Resolution Imaging Spectrometer (MOD16) and the ETVIC from Variable Infiltration Capacity Model (VIC). A linear regression equation and statistical indices were used to model performance evaluation. The results showed that the daily ETSEBAL correlated very well with the ET0, ETMOD16, and ETVIC, and bias between the ETSEBAL with them was less than 1.5%. In general, the SEBAL model could provide good estimations in daily ET over the study region. In addition, the spatial-temporal distribution of ETSEBAL was explored. The variation of ETSEBAL was significant in seasons with high values during the growth period of vegetation in March and April and low values in November. Spatially, the daily ETSEBAL values in the mountain area were much higher than those in the plain areas over the study region. The variability of ETSEBAL in this study area was positively correlated with elevation and negatively correlated with surface reflectance, which implies that elevation and surface reflectance are the important factors for predicting ET in this study area.
Green’s Function for Static Klein–Gordon Equation Stated on a Rectangular Region and Its Application in Meteorology Data Assimilation
Authors: Cheng Hao , Mu Xiyu , Jiang Hua , Wei Ming , Liu Guoqing
Published: December 2021

The meteorology data assimilation applications often encounter variational problems with unknown weights, where the corresponding Euler equation is an elliptic partial differential equation. This research focused on retrieving the weights in remote sensing data assimilation by means of the computer-friendly form of the Green’s function obtained by eigenfunction expansion for the boundary value problem of the static Klein–Gordon equation on a rectangular region. With the help of the proposed retrieving method, the assimilation problem of estimating regional precipitation with weather radar and rain-gauge is solved in the Green’s function method. Results show that high accuracy of the proposed method makes it a good candidate for data assimilation problems in operational use.
Isolated Effects of Indian Ocean Basin-Wide and El Niño–Southern Oscillation on Austral Winter Rainfall over South America
Authors: Kayano Mary T. , Cerón Wilmar L. , Andreoli Rita V. , Souza Rodrigo A. F. , Souza Itamara P.
Published: December 2021

Contrasting effects of the tropical Indian and Pacific Oceans on the atmospheric circulation and rainfall interannual variations over South America during southern winter are assessed considering the effects of the warm Indian Ocean basin-wide (IOBW) and El Niño (EN) events, and of the cold IOBW and La Niña events, which are represented by sea surface temperature-based indices. Analyses are undertaken using total and partial correlations. When the effects of the two warm events are isolated from each other, the contrasts between the associated rainfall anomalies in most of South America become accentuated. In particular, EN relates to anomalous wet conditions, and the warm IOBW event to opposite conditions in extensive areas of the 5° S–25° S band. These effects in the 5° S–15° S sector are due to the anomalous regional Hadley cells, with rising motions in this band for the EN and sinking motions for the warm IOBW event. Meanwhile, in subtropical South America, the opposite effects of the EN and warm IOBW seem to be due to the presence of anomalous anticyclone and cyclone and associated moisture transport, respectively. These opposite effects of the warm IOBW and EN events on the rainfall in part of central South America might explain the weak rainfall relation in this region to the El Niño–Southern Oscillation (ENSO). Our results emphasize the important role of the tropical Indian Ocean in the South American climate and environment during southern winter.
Stress and Maladaptive Coping of Italians Health Care Professionals during the First Wave of the Pandemic
Authors: Grandinetti Paolo , Gooney Martina , Scheibein Florian , Testa Roberta , Ruggieri Gaetano , Tondo Paolo , Corona Anastasia , Boi Graziella , Floris Luca , Profeta Valerio F. , G. Wells John S. G. , De Berardis Domenico
Published: December 2021

Stress during the pandemic has had an impact on the mental health of healthcare professionals (HCPs). However, little is known about coping and “maladaptive” coping behaviours of this population. This study investigates “maladaptive” coping behaviours and their correlation with stress, anxiety and insomnia of Italian HCPs during the pandemic. It reports on a cross-sectional, descriptive and correlational study based on a survey of 1955 Italian HCPs. Overall participants reported increases in cigarette smoking, time spent online and video playing. Overall reported alcohol consumption decreased but increased in those reporting drinking more than once a week. Those reporting starting smoking during the pandemic were found to have higher SAS and PSS scores. Those reporting being online for 3 or more hours were found to have higher ISS scores. Doctors who reported playing video games were found to have higher PSS, ISS and SAS scores whilst nurses who reported playing video games were found to have higher ISS scores. Doctors who reported playing for longer than one hour had higher PSS scores. Online behaviours may be a coping behaviour of HCPs affected by the pandemic. However, this is an underexplored area for the wellbeing of HCPs. These deficits need to be addressed going forward.
Brain Responses to High-Calorie Visual Food Cues in Individuals with Normal-Weight or Obesity: An Activation Likelihood Estimation Meta-Analysis
Authors: Yang Yingkai , Wu Qian , Morys Filip
Published: December 2021

Overconsumption of high-calorie or unhealthy foods commonly leads to weight gain. Understanding people’s neural responses to high-calorie food cues might help to develop better interventions for preventing or reducing overeating and weight gain. In this review, we conducted a coordinate-based meta-analysis of functional magnetic resonance imaging studies of viewing high-calorie food cues in both normal-weight people and people with obesity. Electronic databases were searched for relevant articles, retrieving 59 eligible studies containing 2410 unique participants. The results of an activation likelihood estimation indicate large clusters in a range of structures, including the orbitofrontal cortex (OFC), amygdala, insula/frontal operculum, culmen, as well as the middle occipital gyrus, lingual gyrus, and fusiform gyrus. Conjunction analysis suggested that both normal-weight people and people with obesity activated OFC, supporting that the two groups share common neural substrates of reward processing when viewing high-calorie food cues. The contrast analyses did not show significant activations when comparing obesity with normal-weight. Together, these results provide new important evidence for the neural mechanism underlying high-calorie food cues processing, and new insights into common and distinct brain activations of viewing high-calorie food cues between people with obesity and normal-weight people.
MEG Node Degree Differences in Patients with Focal Epilepsy vs. Controls—Influence of Experimental Conditions
Authors: Vogel Stephan , Kaltenhäuser Martin , Kim Cora , Müller-Voggel Nadia , Rössler Karl , Dörfler Arnd , Schwab Stefan , Hamer Hajo , Buchfelder Michael , Rampp Stefan
Published: December 2021

Drug-resistant epilepsy can be most limiting for patients, and surgery represents a viable therapy option. With the growing research on the human connectome and the evidence of epilepsy being a network disorder, connectivity analysis may be able to contribute to our understanding of epilepsy and may be potentially developed into clinical applications. In this magnetoencephalographic study, we determined the whole-brain node degree of connectivity levels in patients and controls. Resting-state activity was measured at five frequency bands in 15 healthy controls and 15 patients with focal epilepsy of different etiologies. The whole-brain all-to-all imaginary part of coherence in source space was then calculated. Node degree was determined and parcellated and was used for further statistical evaluation. In comparison to controls, we found a significantly higher overall node degree in patients with lesional and non-lesional epilepsy. Furthermore, we examined the conditions of high/reduced vigilance and open/closed eyes in controls, to analyze whether patient node degree levels can be achieved. We evaluated intraclass-correlation statistics (ICC) to evaluate the reproducibility. Connectivity and specifically node degree analysis could present new tools for one of the most common neurological diseases, with potential applications in epilepsy diagnostics.
“Hard to Say, Hard to Understand, Hard to Live”: Possible Associations between Neurologic Language Impairments and Suicide Risk
Authors: Costanza Alessandra , Amerio Andrea , Aguglia Andrea , Magnani Luca , Serafini Gianluca , Amore Mario , Merli Roberto , Ambrosetti Julia , Bondolfi Guido , Marzano Lisa , Berardelli Isabella
Published: December 2021

In clinical practice, patients with language impairments often exhibit suicidal ideation (SI) and suicidal behavior (SB, covering the entire range from suicide attempts, SA, to completed suicides). However, only few studies exist regarding this subject. We conducted a mini-review on the possible associations between neurologic language impairment (on the motor, comprehension, and semantic sides) and SI/SB. Based on the literature review, we hypothesized that language impairments exacerbate psychiatric comorbidities, which, in turn, aggravate language impairments. Patients trapped in this vicious cycle can develop SI/SB. The so-called “affective prosody” provides some relevant insights concerning the interaction between the different language levels and the world of emotions. This hypothesis is illustrated in a clinical presentation, consisting of the case of a 74-year old woman who was admitted to a psychiatric emergency department (ED) after a failed SA. Having suffered an ischemic stroke two years earlier, she suffered from incomplete Broca’s aphasia and dysprosody. She also presented with generalized anxiety and depressive symptoms. We observed that her language impairments were both aggravated by the exacerbations of her anxiety and depressive symptoms. In this patient, who had deficits on the motor side, these exacerbations were triggered by her inability to express herself, her emotional status, and suffering. SI was fluctuant, and—one year after the SA—she completed suicide. Further studies are needed to ascertain possible reciprocal and interacting associations between language impairments, psychiatric comorbidities, and SI/SB. They could enable clinicians to better understand their patient’s specific suffering, as brought on by language impairment, and contribute to the refining of suicide risk detection in this sub-group of affected patients.
Molecular Activation of the Kv11.1 Channel Reprograms EMT in Colon Cancer by Inhibiting TGFβ Signaling via Activation of Calcineurin
Authors: Eskandari Najmeh , Senyuk Vitalyi , Moore Jennifer , Kalik Zane , Luan Qiyue , Papautsky Ian , Moshiri Arfa , Bocchetta Maurizio , Salami Seyed Alireza , Oryan Shahrbanoo , Gentile Saverio
Published: December 2021

Control of ionic gradients is critical to maintain cellular homeostasis in both physiological and pathological conditions, but the role of ion channels in cancer cells has not been studied thoroughly. In this work we demonstrated that activity of the Kv11.1 potassium channel plays a vital role in controlling the migration of colon cancer cells by reversing the epithelial-to-mesenchymal transition (EMT) into the mesenchymal-to-epithelial transition (MET). We discovered that pharmacological stimulation of the Kv11.1 channel with the activator molecule NS1643 produces a strong inhibition of colon cancer cell motility. In agreement with the reversal of EMT, NS1643 treatment leads to a depletion of mesenchymal markers such as SNAIL1, SLUG, TWIST, ZEB, N-cadherin, and c-Myc, while the epithelial marker E-cadherin was strongly upregulated. Investigating the mechanism linking Kv11.1 activity to reversal of EMT into MET revealed that stimulation of Kv11.1 produced a strong and fast inhibition of the TGFβ signaling. Application of NS1643 resulted in de-phosphorylation of the TGFβ downstream effectors R-SMADs by activation of the serine/threonine phosphatase PP2B (calcineurin). Consistent with the role of TGFβ in controlling cancer stemness, NS1643 also produced a strong inhibition of NANOG, SOX2, and OCT4 while arresting the cell cycle in G0/G1. Our data demonstrate that activation of the Kv11.1 channel reprograms EMT into MET by inhibiting TGFβ signaling, which results in inhibition of motility in colon cancer cells.
Additional Value of PET Radiomic Features for the Initial Staging of Prostate Cancer: A Systematic Review from the Literature
Authors: Guglielmo Priscilla , Marturano Francesca , Bettinelli Andrea , Gregianin Michele , Paiusco Marta , Evangelista Laura
Published: December 2021

We performed a systematic review of the literature to provide an overview of the application of PET radiomics for the prediction of the initial staging of prostate cancer (PCa), and to discuss the additional value of radiomic features over clinical data. The most relevant databases and web sources were interrogated by using the query “prostate AND radiomic* AND PET”. English-language original articles published before July 2021 were considered. A total of 28 studies were screened for eligibility and 6 of them met the inclusion criteria and were, therefore, included for further analysis. All studies were based on human patients. The average number of patients included in the studies was 72 (range 52–101), and the average number of high-order features calculated per study was 167 (range 50–480). The radiotracers used were [68Ga]Ga-PSMA-11 (in four out of six studies), [18F]DCFPyL (one out of six studies), and [11C]Choline (one out of six studies). Considering the imaging modality, three out of six studies used a PET/CT scanner and the other half a PET/MRI tomograph. Heterogeneous results were reported regarding radiomic methods (e.g., segmentation modality) and considered features. The studies reported several predictive markers including first-, second-, and high-order features, such as “kurtosis”, “grey-level uniformity”, and “HLL wavelet mean”, respectively, as well as PET-based metabolic parameters. The strengths and weaknesses of PET radiomics in this setting of disease will be largely discussed and a critical analysis of the available data will be reported. In our review, radiomic analysis proved to add useful information for lesion detection and the prediction of tumor grading of prostatic lesions, even when they were missed at visual qualitative assessment due to their small size; furthermore, PET radiomics could play a synergistic role with the mpMRI radiomic features in lesion evaluation. The most common limitations of the studies were the small sample size, retrospective design, lack of validation on external datasets, and unavailability of univocal cut-off values for the selected radiomic features.
Mad2 Induced Aneuploidy Contributes to Eml4-Alk Driven Lung Cancer by Generating an Immunosuppressive Environment
Authors: Alikhanyan Kristina , Chen Yuanyuan , Somogyi Kalman , Kraut Simone , Sotillo Rocio
Published: December 2021

Aneuploidy, an imbalance number of chromosomes, is frequently observed in lung cancer and inversely correlates with patient survival. Paradoxically, an aneuploid karyotype has detrimental consequences on cellular fitness, and it has been proposed that aneuploid cells, at least in vitro, generate signals for their own elimination by NK cells. However, how aneuploidy affects tumor progression as well as the interplay between aneuploid tumor cells and the tumor microenvironment is still unclear. We generated a new mouse model in which overexpression of Mad2 was almost entirely restricted to normal epithelial cells of the lung, and combined it with an oncogenic Eml4-Alk chromosome inversion. This combination resulted in a higher tumor burden and an increased number of tumor nodules compared to control Eml4-Alk mice alone. The FISH analysis detected significant differences in the aneuploidy levels in the non-tumor regions of Eml4-Alk+Mad2 compared to Eml4-Alk alone, although both tumor groups presented similar levels of aneuploidy. We further show that aneuploid cells in the non-tumor areas adjacent to lung tumors recruit immune cells, such as tumor-associated macrophages. In fact, these areas presented an increase in alveolar macrophages, neutrophils, decreased cytotoxic CD8+ T cells, and IFN-γ, suggesting that aneuploid cells in the surrounding tumor areas create an immunosuppressive signature that might contribute to lung tumor initiation and progression.
Targeted Therapies for the Neurofibromatoses
Authors: Sanchez Lauren D. , Bui Ashley , Klesse Laura J.
Published: December 2021

Over the past several years, management of the tumors associated with the neurofibromatoses has been recognized to often require approaches that are distinct from their spontaneous counterparts. Focus has shifted to therapy aimed at minimizing symptoms given the risks of persistent, multiple tumors and new tumor growth. In this review, we will highlight the translation of preclinical data to therapeutic trials for patients with neurofibromatosis, particularly neurofibromatosis type 1 and neurofibromatosis type 2. Successful inhibition of MEK for patients with neurofibromatosis type 1 and progressive optic pathway gliomas or plexiform neurofibromas has been a significant advancement in patient care. Similar success for the malignant NF1 tumors, such as high-grade gliomas and malignant peripheral nerve sheath tumors, has not yet been achieved; nor has significant progress been made for patients with either neurofibromatosis type 2 or schwannomatosis, although efforts are ongoing.
Prognostic Features of Near-Infrared Spectroscopy Following Primary Radical Prostatectomy
Authors: Vermassen Tijl , Himpe Jonas , Coopman Renaat , Van Praet Charles , Lumen Nicolaas , Rottey Sylvie , Delanghe Joris
Published: December 2021

Background: Only a few biomarkers have been evaluated for their prognostic value with regard to biochemical recurrence (BCR) following primary radical prostatectomy. We explored the possibilities of using near-infrared (NIR) spectroscopy as a prognostic biomarker for BCR-free survival (BCR-FS). Methods: Tissue specimens from 82 prostate cancer patients were obtained. Formalin-fixed paraffin-embedded slides (hematoxylin–eosin-stained) were analyzed using NIR spectroscopy. Prognostic features for BCR-FS were determined following normalization of the spectra. Results: Several differences were found throughout the NIR spectrum for the patients with or without BCR, for both the first derivative and second derivative of the NIR spectrum. Following categorization and Cox regression analysis, spectral regions at 5236 cm−1 (first derivative; median BCR-FS not reached versus 3.2 years; HRhigh = 0.18 [0.08–0.39]; and p < 0.0001) and at 5956 cm−1 (second derivative; median BCR-FS not reached versus 3.8 years; HRlow = 0.22 [0.10–0.48]; and p = 0.0002) showed prognostic properties for BCR-FS. The combination of both parameters further increased the prognostic value of NIR (p < 0.0001). Conclusions: We demonstrated NIR spectral variations between patients with or without BCR, which have been shown to have prognostic value. This easy-to-use technique could possibly further improve post-primary radical prostatectomy monitoring and swift referral to adjuvant local therapies. Further elaboration is highly recommended to fully elucidate these variations and to gain a deeper insight into the changing chemical and physical compositions of the prostate tumor architecture.
An Exploratory Analysis of Changes in Circulating Plasma Protein Profiles Following Image-Guided Ablation of Renal Tumours Provides Evidence for Effects on Multiple Biological Processes
Authors: Wah Tze Min , Zhong Jim , Wilson Michelle , Vasudev Naveen S. , Banks Rosamonde E.
Published: December 2021

Further biological understanding of the immune and inflammatory responses following ablation is critical to the rational development of combination ablation-immunotherapies. Our pilot exploratory study evaluated the circulating plasma protein profiles after image-guided ablation (IGA) of small renal masses to determine the resultant systemic effects and provide insight into impact both on the tumour and immune system. Patients undergoing cryotherapy (CRYO), radiofrequency ablation (RFA) or microwave ablation (MWA) for small renal tumours were recruited. Blood samples were obtained at four timepoints; two baselines prior to IGA and at 24 h and 1–3 months post-IGA, and a panel of 164 proteins measured. Of 55 patients recruited, 35 underwent ablation (25 CRYO, 8 RFA, 2 MWA) and biomarker measurements. The most marked changes were 24 h post-CRYO, with 29 proteins increasing and 18 decreasing significantly, principally cytokines and proteins involved in regulating inflammation, danger-associated molecular patterns (DAMPs), cell proliferation, hypoxic response, apoptosis and migration. Intra-individual variation was low but inter-individual variation was apparent, for example all patients showed increases in IL-6 (1.7 to 29-fold) but only 50% in CD27. Functional annotation analysis highlighted immune/inflammation and cell proliferation/angiogenesis-related clusters, with interaction networks around IL-6, IL-10, VEGF-A and several chemokines. Increases in IL-8, IL-6, and CCL23 correlated with cryoprobe number (p = 0.01, rs = 0.546; p = 0.009, rs = 0.5515; p = 0.005, rs = 0.5873, respectively). This initial data provide further insights into ablation-induced biological changes of relevance in informing trial design of immunotherapies combined with ablation.
Dysregulated TET Family Genes and Aberrant 5mC Oxidation in Breast Cancer: Causes and Consequences
Authors: Xu Bo , Wang Hao , Tan Li
Published: December 2021

DNA methylation (5-methylcytosine, 5mC) was once viewed as a stable epigenetic modification until Rao and colleagues identified Ten-eleven translocation 1 (TET1) as the first 5mC dioxygenase in 2009. TET family genes (including TET1, TET2, and TET3) encode proteins that can catalyze 5mC oxidation and consequently modulate DNA methylation, not only regulating embryonic development and cellular differentiation, but also playing critical roles in various physiological and pathophysiological processes. Soon after the discovery of TET family 5mC dioxygenases, aberrant 5mC oxidation and dysregulation of TET family genes have been reported in breast cancer as well as other malignancies. The impacts of aberrant 5mC oxidation and dysregulated TET family genes on the different aspects (so-called cancer hallmarks) of breast cancer have also been extensively investigated in the past decade. In this review, we summarize current understanding of the causes and consequences of aberrant 5mC oxidation in the pathogenesis of breast cancer. The challenges and future perspectives of this field are also discussed.