bluuebunny/crossref_metadata_2025 huggingface dataset:
A Rapid and Affordable Point-of-care Test for Detection of SARS-Cov-2-Specific Antibodies Based on Hemagglutination and Artificial Intelligence-Based Image Interpretation
Authors: Haecker Hans , Redecke Vanessa , Tawaratsumida Kazuki , Larragoite Erin , Williams Elizabeth , Planelles Vicente , Spivak Adam , Hirayama Lincoln , Elgort Marc , Swenson Shane , Smith Rick , Worthen Bryan , Zimmerman Russ , Slev Patricia , Cahoon Ben , Astill Mark
Published: July 2021

Diagnostic tests that detect antibodies (AB) against SARS-CoV-2 for evaluation of seroprevalence and guidance of health care measures are important tools for managing the COVID-19 pandemic. Current tests have certain limitations with regard to turnaround time, costs and availability, particularly in point-of-care (POC) settings. We established a hemagglutination-based AB test (HAT) that is based on bi-specific proteins which contain a dromedary-derived antibody (nanobody) binding red blood cells (RBD) and a SARS-CoV-2-derived antigen, such as the receptor-binding domain of the Spike protein (Spike-RBD). While the nanobody mediates swift binding to RBC, the antigen moiety directs instantaneous, visually apparent hemagglutination in the presence of SARS-CoV-2-specific AB generated in COVID-19 patients or vaccinated individuals. Method comparison studies with assays cleared by emergency use authorization (EUA) demonstrate high specificity and sensitivity. To further increase objectivity of test interpretation, we developed an image analysis tool based on digital image acquisition (via a cell phone) and a machine learning algorithm based on defined sample-training and –validation datasets. Preliminary data, including a small clinical study, provides proof of principle for test performance in a POC setting. Together, the data support the interpretation that this AB test format, which we refer to as ‘NanoSpot.ai’, is suitable for POC testing, can be manufactured at very low costs and, based on its generic mode of action, can likely be adapted to a variety of other pathogens.
Upregulation of CD22 by Chidamide Promotes CAR T Cells Functionality
Authors: Yang Xin , Yu Qiuxia , Xu Hao , Zhou Jianfeng
Published: July 2021

Treatment failure or relapse due to tumor escape caused by reduction in target antigen expression has become a challenge in the field of CART therapy. Target antigen density is closely related to the effectiveness of CART therapy, and reduced or lost target antigen expression limits the efficacy of CART therapy and hinders the durability of CAR T cells. Epigenetic drugs can regulate histones for molecular modifications to regulate the transcriptional, translational and post-translational modification processes of target agents., and we demonstrated for the first time the role in regulating CD22 expression and its effect on the efficacy of CD22 CART. In this paper, we found that Chidamide promoted the expression of CD22 on the surface of B-cell tumor cells in vitro and in vivo, and enhanced the function of CD22 CART. As for mechanisms, we demonstrated that Chidamide did not affect CD22 mRNA transcription, but significantly increased the expression of total CD22 protein, indicating that Chidamide may upregulate cell surface CD22 expression by affecting the distribution of CD22 protein. In summary, our results suggest that Chidamide may enhance the efficacy of CD22 CART by inhibiting histone deacetylases to regulate post-transcriptional modifications that affect protein distribution to increase the expression of CD22 on the cell surface.
Research on the Geothermal Resource Exploration in Igneous Rock Areas by Comprehensive Geophysical Methods
Authors: Zhu Jie , Jin Sheng , Peng Sanxi , Yang Yang , Zhang Tianyu , Zeng Weizu
Published: July 2021

Geothermal resource has considerable development potential and utilization value. In order to have a clearer understanding and understanding of geothermal resources, it is very important to carry out geological exploration of geothermal resources before mining. In Igneous rocks, ground water is heated by deep magmatic rocks and Igneous rocks to form geothermal resources, but their communication is poor and the depth of burial is generally large. The traditional geophysical method, such as the excitation polarization method, is difficult to reach the effective detection depth, and the precision is poor. In this paper, the comprehensive geophysical method based on CSAMT and TEM is applied to geothermal exploration in igneous rock area, which makes up for the shortcomings of single method and achieves good detection effect, which provides reliable geological basis for further development and utilization of geothermal resources.
Correlation of Fibromyalgia Survey Questionnaire and Quantitative Sensory Testing Among Patients With Active Rheumatoid Arthritis
Authors: Moore Meriah N. , Wallace Beth I. , Song Jing , Muhammad Lutfiyya N. , Heisler Andrew C. , Clauw Daniel J. , Bolster Mary B. , Marder Wendy , Neogi Tuhina , Wohlfahrt Alyssa , Dunlop Dorothy D. , Lee Yvonne C.
Published: July 2021

BackgroundPatients with rheumatoid arthritis (RA) commonly demonstrate disordered pain processing, termed pain centralization. Heightened pain sensitivity and pain pathways associated with pain centralization are often assessed using quantitative sensory testing (QST), which is not routinely available and burdensome to patients. The self-administered fibromyalgia survey questionnaire (FSQ) has been proposed as a low-burden, surrogate measure of pain centralization. We examined the correlation between FSQ and QST as measures of pain centralization in a cohort of patients with active RA. MethodsParticipants in the multicenter Central Pain in Rheumatoid Arthritis (CPIRA) cohort underwent FSQ and QST evaluation at enrollment. QST measures included pressure pain threshold (PPT) at the thumb, trapezius, wrist and knee; temporal summation (TS) at the wrist and arm; and conditioned pain modulation (CPM). Partial Spearman correlation was assessed between FSQ and each QST measure, adjusted for age, sex, race, body mass index, serologic status, swollen joint count, C-reactive protein, pain catastrophizing, and study site. We performed sensitivity analyses including a) stratified analysis by sex and b) correlations between the two sub-components of the FSQ (widespread pain index [WPI], and symptom severity scale [SSS]) and QST measures.ResultsAmong 285 participants with high RA disease activity (mean baseline Clinical Disease Activity Index score 24.56), FSQ was weakly but statistically significantly correlated with PPT ( r = -0.21 to -0.31) and TS ( r = 0.13 to 0.15) at all sites in unadjusted analyses. After adjustment, statistically significant correlations persisted for PPT at all sites except the thumb, and for TS at the wrist Sensitivity analyses did not identify differences in association based on sex or with the components of the FSQ (WPI or SSS).ConclusionsFSQ and QST were correlated among participants with active RA, but the strength of association was modest. This may be due to the high level of RA disease activity of the cohort studied. Both QST and the FSQ are likely imperfect singular measures of pain centralization.
Milk Carbon Footprint in Latin American Livestock: effect of dairy system, breed and climate
Authors: Velarde-Guillén José , Arndt Claudia , Gomez Carlos
Published: July 2021

The study reviewed carbon footprint (CF) analyses for milk production in Latin America from cradle to farm gate. The objective was to estimate: (1) the effect of production system (zero-grazing, mixed, and pasture), (2) animal breed (specialized dairy vs. dual-purpose), and (3) climate (tropical vs. temperate) on milk production (kg/cow/day) and CF [kg CO2eq/kg fat and protein corrected milk (FPCM)]. A systematic literature review was conducted, in which 11 studies which estimate 32 individual CF were included in the final analysis. Studies included in the final analysis allowed to calculate CF per kg FPCM, included upstream emissions calculations, and used a Tier 2 approach for enteric methane emissions. The range of the CF observed in the region was of 1.54 to 3.57 kg CO2eq/kg FPCM. The production system had a significant effect on milk production, but not on CF. Zero-grazing compared with pasture systems had a 140% greater milk production (20.1 vs. 8.4 kg of milk/cow/day), but numerically greater CF (70%) for pasture systems. Compared with specialized dairy cattle, dual-purpose breeds produced less milk and higher CF. Compared with temperate climate, tropical climate systems produced less milk and higher CF. In conclusion, in Latin America the breed selection and the climate have more impact in the CF that the dairy production system.
Mucormycosis, COVID-19 and Acute Lymphoid Leukemia: Case Report
Authors: Cordero-Hernández Edgar L. , Martínez-Murillo Carlos , Barranco-Lampón Gilberto , Arias-Castro Itsel , Santoyo-Sánchez Adrián , Ramos-Peñafiel Christian O
Published: July 2021

Introduction: During the era of COVID-19, an increase has surged in the number of cases of mucormycosis; the use of high doses of steroids, and states of immunosuppression such as diabetes are the main related risk factors. In the treatment of acute leukemia, it is recommended to reduce the dose of chemotherapy, postponing highly myeloablative schemes and the use of caution with monoclonal antibodies. Case presentation: We present the case of a patient (25 years-old male) with acute lymphoblastic leukemia and COVID-19 confirmed infection who developed rhino-cerebral mucormycosis during the induction stage. Conclusion: In the age of COVID-19, taking samples through a nasal swab should be avoided in individuals with risk of infection from mucormycosis, such as diabetes, cancer, or individuals treated with high doses of steroids.
Oryza Longistaminata-like Cryptic Species Found in the Mekong Delta Probably Involved in the Origin of O. Rufipogon Indica Ancestor
Authors: Htut Htet Aung , Makabe So , Takahashi Hiroko , Buu Bui Chi , Lang Nguyen Thi , Samuel Poku Aduse , Ishikawa Ryuji , Nakamura Ikuo
Published: July 2021

BackgroundOryza sativa L. contains two cultivated subspecies, indica and japonica, which are considered to be derived from wild rice relative, O. rufipogon. Although domestication cinario of O. sativa has been extensively studied, it is still inconclusive and contradictory. Moreover, it remains to be revealed why O. rufipogon had acquired wide range of genetic diversity to fit various habitat and life cycle of plant from annual to perennial.ResultsAlthough O. rufipogon distributes in southeast Asia from Indonesia to India, perennial wild rice strains were distributed in deep-water area of the Mekong Delta in Southern Vietnam. Some of wild rice strains collected in Dong Thap were heterologous in the length of intron 20 sequence of single-copy nuclear PolA1 gene, encoding for the largest subunit of RNA polymerase I. Thus, these wild rice strains were probably considered as interspecific hybrids, because the S-type (0.14 kb) intron 20 was found in O. rufipogon, O. glumaepatula, and O. barthii while L-type (1.5 kb) was shared by O. longistaminata and O. meridionalis. Therefore, we identified two wild rice strains that contained homologous L-type intron 20 of PolA1 gene in the CLRRI collection. These two strains, named as MD1 and MD2, had fertile spikelet with long anthers which were typical feature of African O. longistaminata. In addition, MD1 strain and O. longistaminata shared the same protein tag (Ptag) sequence, which was different to that of O. rufipogon and O. sativa. Interestingly, MD1 strain contained plastid DNA with a 69-bp deletion in ORF100 which was shared with indica and annual O. rufipogon, but not with japonica and perennial O. rufipogon. ConclusionWe have identified that MD strains found in the Mekong Delta were probably O. longistaminata-like cryptic species and contributed to enlarge genetic diversity of O. rufipogon, and is probably involved in the origin of O. rufipogon indica ancestor of as the maternal lineage.
MKL1/PINK1-AS/miR-34a-5p Regulates ALDOA to Affect Aerobic Glycolysis in Hepatocellular Carcinoma
Authors: Wang Jun , Zhang Huiming , Dai Zhoutong , Huang You , Liu Hui , Chen Zhen , Wu Yuan , Zhang Tongcun , Liao Xinghua
Published: July 2021

BackgroundAldolase A, an important metabolic enzyme in the glycolytic pathway, plays an important role in regulating tumour metabolism. In this study, we investigated the expression pattern of ALDOA in hepatocellular carcinoma and its biological role in tumour progression.MethodsBioinformatics analysis, Western Blot and RT-qPCR were performed to detect the relative expression of ALDOA in hepatocellular carcinoma tissues and cell lines. A loss-of-function approach was used to investigate the biological function of ALDOA. The role of ALDOA on glycolysis and apoptosis was assessed by Western Blot, glucose and lactate assay kits, flow cytometry and a nude mouse xenograft model. RNA immunoprecipitation, luciferase reporter experiment, chromatin immunoprecipitation and Western Blot were performed to elucidate the underlying molecular ResultsElevated ALDOA expression correlated with the prognosis of hepatocellular carcinoma patients. exogenous downregulation of ALDOA expression significantly inhibited cellular glycolysis and promoted apoptosis. Mechanistic studies suggest that ALDOA is a direct target of miR-34a-5p, which can enhance apoptosis and inhibit glycolysis in hepatocellular carcinoma cells by targeting the 3'UTR of ALDOA. PINK1-AS competitively sponged miR-34a-5p to increase ALDOA expression by antagonizing miR-34a-5p-mediated ALDOA inhibition. MKL1 acted as a transcription factor to promote the expression of PINK1-AS and ALDOA, thus promoting the deterioration of hepatocellular carcinoma cells.ConclusionThis study shows that high expression of ALDOA contributes to the development and poor prognosis of hepatocellular carcinoma and will be a target and potential prognostic biomarker for the treatment of hepatocellular carcinoma.
Growth Hormone-Like Activities of Organophosphorus Flame Retardants (Opfrs) in Growth of Rice (Oryza Sativa L.)
Authors: Bai Qian , Wang Haiou , Zhang Wenxiao , Jiang Bokai , Ma Yuqing , Li Yuetong , Shi Hanbo
Published: July 2021

To identify the toxic effect of Organophosphorus Flame Retardants (OPFRs) on plants, six different OPFRs involved in three concentrations (50 mg/L, 100 mg/L and 200 mg/L) were selected to estimate their toxicity to rice growth. The seed germination, seedling growth, OPFRs accumulation and antioxidative defense system were investigated in rice. The results showed that all of OPFRs inhibited the germination of seeds. OPFRs were accumulated in roots and shoots, and the accumulations of OPFRs in roots were higher than in shoots. However, the hormone-like effect of all six OPFRs was found on plants. The growth of seedlings was irrigated by 50 mg/L, 100 mg/L and 200 mg/L OPFRs in our experiment according to fresh weights and lengths of seedlings. And then malondialdehyde (MDA) and antioxidative defense system were impressed after OPFRs exposed 21 d. According to gene expressions of seven antioxidative enzymes at 14 d, most of enzyme expressions were updated to alleviated reactive oxygen species (ROS) by OPFRs exposure. Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP) showed the strongest oxidative toxicity to plants among all of OPFRs. During the early 14 d period, the antioxidant enzymes could play important role in detoxification process. And after 21 d, antioxidants ascorbate (AsA) in roots and glutathione (GSH) in shoots could take over antioxidant enzymes against to OPFRs toxicity.
Molluscicidal and Antioxidant Activities of Silver Nanoparticles on the Snail Intermediate Hosts of Schistosomiasis
Authors: Zayed Khaled M. , Guo Yun-Hai , Zhou Xiao-Nong
Published: July 2021

Background: Schistosomiasis, also known as bilharzia, is a disease caused by parasitic worms. This disease is the second important parasitic disease after malaria in terms of its burden of disease. Methods: One of control intervention for schistosomiasis control programme is to perform the mollusciciding to interrupt the transmission through snail intermediate host where the parasites developed from miracidia to cercaria that is the infective stage. Accordingly, it is possible to improve the molluscicding effects after adding nanoparticulate agents in molluscicides. Results: This research field has caused more attention, since silver nanoparticles (Ag-NPs) were characterized by the transmission electron micrographs (TEM) with X-Ray fluorescence (XRF), showed that it’s mostly spherical in shape with average particle size diameters 20nm with high purity of synthesized particles (99.99%) of element was Ag. The aim of the present study is to demonstrate the molluscicidal effects of Ag-NPs against the three species of snails, including two species of intermediate snail host of Schistosoma mansoni (Biomphalaria alexandarina and Biomphalria glabrata) and one species of intermediate snail host of S. japonicum (Oncomelania hupensis), and the cercaricidal effects of Ag-NPs against Schistosoma mansoni cercariae. At the same time, their effects on snail’s antioxidant enzymes, e.g. catalase (CAT), glutathione reduced (GSH), nitric oxide (NO) and total antioxidants capacity (TAC), and on freshwater cladoceran (crustacean) Daphnia magna that represent the potential toxicity to other aquatic organisms were observed as well. The results indicated that Ag-NPs had a molluscicidal effect on tested snails with the variation in lethal concentration dose. LC50 of Ag-NPs exposure for 24, 48, 72 hrs and 7 days to B. alexandrina snails was 7.91, 5.69, 3.83 and 1.91 ppm respectively, LC50 for 24, 48, 72 hrs and 7 days exposure to B. glabrata snails was 16.55, 10.44, 6.91 and 4.13 ppm respectively, while LC50 for 24, 48, 72 hrs and 7 days exposure to Oncomelania hupensis snails was 46.5, 29.85, 24.49 and 9.62 ppm respectively. While the cercaricidal effects on S. mansoni cercariae was fast, since the lethal time LT50 and LT90 exposed to Ag-NP with 80 ppm concentration was 27 min and 45 min, respectively; and its 100 ppm exposed was 15min and 41 min, respectively. Moreover, there is no mortality rate to Daphnia magna when exposed to more than double and half dose (50 ppm) of Ag-NPs for continuous 3 hs, of which LC90 to B. alexandarina snails was 18 ppm. Conclusions: The synergistic effect of synthesized Ag-NPs as molluscicidal and cercaricidal was clearly revealed that it’s promising to be effectively used against various intermediate host snails of intestinal schistosomes and reduced it’s a potential antioxidant activity.
Design of Artificial Neural Networks for the Novel Applications of the Sixth Order Singular Nonlinear Pantograph Engineering Model
Authors: Sabir Zulqurnain , Ali Mohamed , Raja Muhammad Asif Zahoor , Sadat R.
Published: July 2021

This aim of this study is to design and numerically investigate a novel nonlinear singular sixth order (SSO) pantograph differential system (PDS), i.e., SSO-PDS along with its five categories. The design of the novel nonlinear SSO-PDS is presented using the concepts of the standard Emden-Fowler along with the pantograph differential system. The singular systems are applied in many engineering and mathematical applications, like inverse models and viscoelasticity systems. The shape factor, singular point and pantograph terms are provided for each category of the novel nonlinear SSO-PDS. The numerical investigations of the nonlinear SSO-PDS are presented through the artificial neural networks (ANNs) using the Levenberg-Marquardt backpropagation (LMB), i.e., ANNs-LMB. The proposed results have been compared with the reference solutions to check the correctness of the designed novel model. The proposed solutions of the novel SSO-PDS are assessed using the training, testing and verification procedures in the form of mean square error (MSE). For the capability, efficacy and accuracy of the novel SSO-PDS through the ANNs, the numerical results are drawn using the MSE, error histograms and regression as well as correlation graphs.
Artificial Intelligence-Based Assistance in Clinical 123I-FP-CIT SPECT Scan Interpretation
Authors: Wolfswinkel Evander van , Wielaard Jette , Lavalaye Jules , Hoff Jorrit , Booij jan , de wit Tim , Habraken Jan
Published: July 2021

Purpose: Dopamine transporter (DAT) imaging with 123I-FP-CIT SPECT is used to support the diagnosis of Parkinson’s disease (PD) in clinically uncertain cases. Previous studies showed that automatic classification of 123I‑FP‑CIT SPECT images (marketed as DaTSCAN) is feasible by using machine learning algorithms. However, these studies lacked sizable use of data from routine clinical practice. This study aims to contribute to the discussion whether artificial intelligence (AI) can be applied in clinical practice. Moreover, we investigated the need for hospital specific training data.Methods: A convolutional neural network (CNN) named DaTNet-3 was designed and trained to classify DaTSCAN images as either normal or supportive of a dopaminergic deficit. Both a multi-site data set (n = 2412) from the Parkinson’s Progression Marker Initiative (PPMI) and an in-house data set containing clinical images (n = 932) obtained in routine practice at the St Antonius hospital (STA) were used for training and testing. STA images were labeled based on interpretation by nuclear medicine physicians. To investigate whether indeterminate scans effects classification accuracy, a threshold was applied on the output probability.Results: DaTNet-3 trained with STA data reached an accuracy of 89.0% in correctly identifying images of the clinical STA test set as either normal or with decreased striatal DAT binding (98.5% on the PPMI test set). When thresholded, accuracy increased to 95.7%. This increase was not observed when trained with PPMI data, indicating the incorrect images were confidently classified as the incorrect class.Conclusion: Based on results of DaTNet-3 we conclude that automatic interpretation of DaTSCAN images with AI is feasible and robust. Further, we conclude DaTNet-3 performs slightly better when it is trained with hospital specific data. This difference increased when output probability was thresholded. Therefore we conclude that the usability of a data set increases if it contains indeterminate images.
Expression and Localization of C-APP and N-APP in Peripheral Neurons of Rats and Crayfish After Axotomy
Authors: Rodkin Stanislav Vladimirovich , Dzreyan Valentina Aleksandrovna , Khaitin Andrey Mikhailovich , Pitinova Maria Aleksandrovna , Eid Moez Ali , Demyanenko Svetlana Viktorovna
Published: July 2021

Nerve injury induces a cascade of molecular-cellular events, leading to neuronal death or survival, where amyloid precursor protein (APP) and its proteolytic products play an important role. We studied the localization and expression of C-APP and N-APP in rat dorsal root ganglia (DRG) with transected sciatic nerve, axotomized crayfish stretch receptor neuron (SRN) and ventral nerve cord (VNC) ganglia with transected connectives. C-APP and N-APP localized predominantly in neurons, not in glial cells. Axotomy increased C-APP and N-APP expression in rat and crayfish neurons. The expression of APP in crustaceans confirms its conservative nature. In DRG, C-APP level was higher in neuronal nuclei than in cytoplasm in 24 hours post-axotomy. N-APP accumulation was not observed in DRG and crayfish neuronal nuclei. SRN axotomy resulted in C-APP and N-APP accumulation in 4–8 hours in perikaryon and its extensions, but only С-APP accumulated in nuclei. This indicates that not the whole APP, but its C-terminal product, AICD, enters the nucleus. Also, there was high level of C-APP in SRN nucleolus, suggesting possible AICD involvement in rRNA synthesis and ribosome formation. The APP accumulation in transected axons confirms its involvement in injury-induced axonal events.
Hydrochemical Characteristics and Formation Mechanism of Groundwater in West Zoucheng City, Shandong Province, China
Authors: Chen Hao , Wang Jiading , Zhang Fei , Zhou Yaxing , Xia Chunying , Zhang Wenliang , Meng Xianzhou , Meng Jia
Published: July 2021

The groundwater in the western part of Zoucheng is an important water source for domestic, industrial and agricultural use in Zoucheng. Understanding its hydrochemical characteristics and formation mechanism is of great significance to the sustainable development and utilization of groundwater. In this study, 36 water samples were collected during wet and dry seasons, and the hydrochemical components K+, Na+, Ca2+, Mg2+, Cl-, SO42-, HCO3-, NO3-, F-, TH and TDS were analyzed. Graphic method, correlation analysis and principal component analysis were applied to explore the hydrochemical characteristics and evolution mechanism of groundwater in the study area. The results show that: (1) The orders of the anion and cation concentrations of karst groundwater and pore groundwater are Ca2+>Na+>Mg2+>K+ and HCO3->SO42->Cl->NO3->F-. (2) On the whole, the water quality from different sources follows a specific order: karst groundwater > pore groundwater > surface water; also, water quality in the dry season is better than that in the wet season from all three sources. (3) The water chemistry types of groundwater are complex and changeable. Compared with dry seasons, HCO3 and SO4 type water increases in wet seasons, while the Cl type and Mg type water decreases. Na type in pore groundwater is significantly more than that in karst groundwater. (4) The chemical formation of karst groundwater and pore groundwater in dry and wet seasons are mainly affected by water-rock interactions and human activities.
Externalising Behaviour in Children: An Integrative Model Between Health and Education
Authors: Rungan Santuri , Montgomery Alicia , Smith-Merry Jennifer , Liu Huei Ming , Eastwood John
Published: July 2021

Background: Externalising behaviours are the most common mental health disorder of childhood and adolescence. They are defined as attention deficit and hyperactivity disorder (ADHD) and conduct disorder (CD). CD is associated with high societal and economic burden. Yudi Gunyi School caters for students aged 10-16 years with problematic externalising behaviour. A multidisciplinary health assessment has been developed. This evaluation aimed to see if this model of care improved the outcomes for children with externalising behaviour.Methods: A retrospective evaluation of all students attending the health assessment between 26 July 2016 and 14 May 2019 using de-identified data was conducted. Descriptive statistics (proportion, mean, SD, and range) were used. A student’s t-test was used to assess change in SDQ scores.Results: Prior to the assessment 22.8% had a paediatrician but 33.3% were not engaged with the paediatrician. Similarly, mental health services were involved in 27.8% but 18.2% were not engaged. Child protection services had previous involvement with 43% and current involvement with 32.9%.Attendance was high (failure to attend 7.6%; cancellation 8.9%). New diagnoses of ADHD (3.8%), autism (1.3%), CD (1.3%) and ODD (1.3%) were made. Other issues identified included learning difficulties (12.7%), medical diagnoses (10.1%), emotional concerns (16.5%), domestic violence (12.7%), OOHC (7.6%), trauma background (27.8%), the death/loss of a close relative/friend (8.9%), parental separation (31.6%) and substance use (19%). SDQ teacher reports showed a statistically significant decrease in total difficulties scores (M = 6.2, SD = 6.165, p < 0.05, eta squared = 1.013 (large effect)) and all subsets including behavioural/conduct difficulties (M = 1.8, SD = 2.4, p <0.05, eta squared = 0.741 (medium effect)), hyperactivity and concentration difficulties (M = 2.8, SD = 2.4, p <0.05, eta squared 1.15 (large effect)) and impact scores (M = 1.636, SD = 1.8, P <0.05, eta squared = 0.909 (large effect)).There were no significant differences in the parent and self-reported SDQs. Conclusions: The multidisciplinary model between health and education offered mechanisms to reduce barriers to care for children experiencing externalising behaviours.
Use of Lead Rubber Bearing for Base Isolation Technique to Control Seismic Force in RC Framed Structures
Authors: M Ashwini R , Rathnamala G V , N Ramya , .S Pradeepa , J Anitha , Selvi N Tamil , M Potharaju , Shivashankara G. P.
Published: July 2021

Seismic waves which are formed during an earthquake carries enormous force that transfers to the base of the structures in different directions. Due to this, the building structure undergoes drastic changes in existing stresses and strains. Mechanisms that are responsible for the damages in structures during an earthquake are acceleration of floors and inter story drift. The traditional ways of resisting the foresaid force are shear walls, moment resisting frames and braced frames etc. It is documented frequently that; the traditional methods are not completely effective to absorb the earthquake force. Therefore, Seismic isolation concept is an effective rehabilitation strategy that is in practice now and gives favorable results. In this work, 6 x 3 Bay with 14 Stories (G +13) RCC building structure incorporated with Lead Rubber Bearing (flexible base isolation) and fixed base have been modeled and analyzed for earthquake force along with other active forces using FEM program. The structural quantities, time history investigations and response spectrum examinations have been compared for both base isolation and fixed base models. It is found that the technique adopted decreases the seismic response considerably compared to conventional (fixed base) structures which intern controls the damages in building. Bases shear and floor accelerations have been reduced but displacements increased in isolated base when compared with fixed base.
Influence of Different Annealing Atmosphere Nature on the Properties of Nanoparticles CdS Thin Films Synthesized By Chemical Bath Method.
Authors: MELOUKI M. , LARBAH Y. , DJELLOUL A. , ADNANE M.
Published: July 2021

The Cadmium sulfide (CdS) is the most advantageous material for the manufacture of the elaborate solar cells in thin layers, the study that we present, will relate to the elaborated and the characterization of CdS thin film deposited on glass substrates by chemical bath deposition (CBD) method. This study will help us to know if the annealing atmosphere nature affects the deposition of thin films of CdS. The X-ray diffraction (XRD) analysis reveals that the structures of pure thin films are Hexagonal and polycrystalline with preferential orientation (002). The scanning electron microscopy (SEM) measurements showed that the surface morphology homogeneous and uniform. The energy dispersive X-ray analysis (EDAX) studies confirmed that the films are nearly stoichiometric. The transmittance in the visible region (200-800 nm) is high of 60%, and band gap values oscillated between 2.36 and 2.47 eV for al thin films.
A Co-Delivery Strategy of Abemaciclib Combined with Vitamin E Succinate Based on Hyaluronic Acid Modified Calcium Phosphate to Enhance the Anti-Cancer Effect
Authors: Liu Yuanyuan , Gan Na , Zhang Shuangshuang , Sun Qiaomei , Wei Xin , Ren Xiuyun , Xiang Xi , Li Hui
Published: July 2021

Combination therapy including anticancer drugs usually induces synergistic effects, especially in combination with natural compounds as a chemical sensitizer. Nano-based drug delivery technology is also effective in solving the toxicity of single drugs at high doses. In this study, we established a co-delivery strategy for abemaciclib (ABE) combined with vitamin E succinate (VES) based on HA-modified calcium phosphate (CaP) nanomaterials to enhance anti-cancer effect. The nano-sized HA/CaP particles (90 nm) have a hollow structure. Surprisingly, after modification with HA, HA/CaP has more pH sensitivity. CaP released 30% ABE and 91% VES at pH 7.4, whereas HA/CaP vsreleased 14% ABE and 32% VES at the same pH. Cell experiments showed that HA/CaP/ABE-VES had stronger cell inhibitory effect on MCF-7 compared with CaP/ABE-VES. This inorganic/organic composite for the co-delivery of ABE and VES has obvious tumor inhibition effect, and is ideal for anti-tumor drug delivery.
A Frequency-based Parent Selection for Reducing the Effect of Evaluation Time Bias in Asynchronous Parallel Multi-objective Evolutionary Algorithms
Authors: Harada Tomohiro
Published: July 2021

This paper proposes a new parent selection method for reducing the effect of evaluation time bias in asynchronous parallel evolutionary algorithms (APEAs). APEAs have the advantage of increasing the computational efficiency even when the evaluation times of solutions differ. However, APEAs have a problem that their search direction is biased toward the search region with a short evaluation time. The proposed parent selection method considers the search frequency of solutions to reduce such an adverse influence of APEAs while maintaining their computational efficiency. We conduct experiments on toy problems that reproduce the evaluation time bias on multi-objective optimization problems to investigate the effectiveness of the proposed method. The experiments use NSGA-III, a well-known multi-objective evolutionary algorithm. In the experiments, we compare the proposed method with the synchronous and the asynchronous methods. The experimental results reveal that the proposed method can reduce the effect of the evaluation time bias while reducing the computing time of the parallel NSGA-III.
Impacts of Maternal Genotype on Pecan Seedling Performance in an Alkaline, Saline-sodic Soil
Authors: Smith Cyrus A. , Walworth James L. , Comeau Mary J. , Heerema Richard J. , Sherman Joshua D. , Norton Randall
Published: July 2021

A field study was conducted to evaluate tolerance of pecan rootstocks to soil salinity and sodicity. Seven cultivars—Elliott, Giles, Ideal, Peruque, Riverside, ‘Shoshoni, and VC1-68—were selected from a range of geographic regions of origin. The soil of the experimental plot was a poorly drained, saline–sodic Pima silty clay variant. The irrigation water was a moderately saline mix of Gila River and local groundwater with an electrical conductivity of 2.8 dS⋅m–1, containing primarily ions of Na and Cl. Eighty seeds of each cultivar were planted in a greenhouse in late Feb. 2016; 48 seedlings of each cultivar were transplanted into field plots in Feb. 2017. Half the trees received a soil-based application of Zn–ethylenediaminetetraacetic acid (EDTA) at planting. The trees were observed and rated for both vigor and resistance to salt injury on seven separate occasions. Trunk diameter was measured each dormant season. Leaf samples were collected on 9 Oct. 2019 and 6 Oct. 2020, and were analyzed for nutrient content. Zn-EDTA was not found to have a significant effect on growth, vigor, or resistance to salt injury. ‘Elliott’ seedlings exhibited greater tolerance for the alkaline, saline–sodic soil conditions than other cultivars. ‘Giles’ and ‘Peruque’ were most severely affected. Resistance to salt injury (ranging from marginal leaf burn to necrosis of entire leaf), vigor, and growth correlated more strongly with foliar concentrations of Na than Cl or K during 2019. Vigor and growth were not significantly correlated with foliar Na, Cl, or K concentrations in 2020. The foliar K:Na ratio had a nearly equal correlation with resistance to salt injury and a greater correlation with growth than that of Na alone in 2019. However, although the correlation of the K:Na ratio with vigor was stronger than that of Cl or K, Na had the strongest correlation with vigor in 2019. In 2020, the only significant correlation of growth and vigor was with the K:Na ratio. The strongest correlation with resistance to salt injury in 2020 was with foliar Na concentration.
Representações discursivas sobre internacionalização por professores de inglês em formação continuada
Authors: Machado Pereira Lauro Sérgio , Da Silva Kléber Aparecido
Published: July 2021

Neste artigo, investiga-se as representações discursivas sobre internacionalização da educação por professores de inglês da Rede Federal de Educação Profissional, Científica e Tecnológica (RFEPCT) participantes do Programa SETEC-CAPES/NOVA de formação continuada. Os pressupostos teórico-metodológicos orientam-se pela Análise de Discurso Crítica (FAIRCLOUGH, 2016 [1992]; 2003) e pela Internacionalização Crítica (VAVRUS; PEKOL, 2015; STEIN, 2019), que, respectivamente, encontram pontos de intersecção na mudança social por intermédio do discurso como prática social e nos princípios da ética, da equidade e da justiça social. Um questionário estruturado online foi aplicado em um grupo de professores de inglês a fim de possibilitar a geração dos dados. As respostas dos docentes participantes sinalizaram representações de internacionalização pautadas por aspectos benéficos do processo, evidenciando discursos hegemônicos e contra-hegemônicos. O estudo possibilitou compreender que delimitar hegemonias e contra-hegemonias nas representações discursivas dos professores participantes acerca da internacionalização é tarefa complexa, pois nessas representações coexistem sentidos híbridos e conflitivos.
Critical Care Management of an Obese Asthmatic Patient: A Review
Authors: Mendez Yamely , Covantev Serghei , Longlax Santiago Carrero , Hernandez Rogelio , Guerrero Ismael García , Moya Stephanie González
Published: July 2021

Obesity and asthma are two conditions that are considered a global health problem.
There has been an increased number of obese patients and different factors contribute to the development
of this disease, such as changes in lifestyle, poor physical activity, and genetics. Obesity,
as a co-morbidity, could contribute to the aggravation of asthma or the progress and exacerbation.
This could lead to asthma-related hospitalizations and ICU admissions. Understanding the pathophysiology
of those two diseases is helpful in the decision-making process of the management. The
main goal of the therapy is reducing the exacerbation symptoms and or the ICU length-stay, which
could depend on the type of mechanical ventilation used on the patient.
Application of graphene oxide-based hydrogels in bone tissue engineering
Authors: Zhihui Kuang , Licheng Ni , Jianxiang Xu , Shining Xiao , Jinwei Lu , Chenwei Zhou , Zhijun Pan
Published: July 2021

:

This paper provides a review of the applications of some graphene oxide-based hydrogels in bone tissue engineering. Hydrogels used in bone tissue engineering usually require suitable biocompatibility, porosity, and mechanical strength. Under the original hydrogel condition, by adding GO, the various indexes of the composite hydrogel can reach more suitable conditions for inducing bone defect healing. This article also introduces some high-performance graphene oxide-based hydrogels, which are likely to play an important role in bone tissue engineering in the future.
Diagnostic Value of Multiplex Polymerase Chain Reaction in Detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from Sepsis in Pediatrics
Authors: Galeb Sara , Zaki Maysaa El Sayed , Shrief Raghdaa , Hassan Rasha , Rizk Mohamed
Published: July 2021

Background:

Proper identification of the causative organism in pediatric sepsis is crucial
for early diagnosis and prevention of septic shock and organ failure.

Objectives:

The aim of the present study was to evaluate the multiplex polymerase chain reaction
(PCR) for detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas
maltophilia from positive blood cultures for these pathogens isolated from children with hospital-
acquired sepsis compared to the conventional biochemical reactions for identification of these
organisms.

Methods:

This study was a cross-sectional study performed on 100 isolates from pediatric blood
cultures, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas
maltophilia. The study also included 100 isolates of Escherichia coli as a negative control. All isolates
were identified by API 20NE and the multiplex PCR with primers specific to the 3 tested bacteria.

Results:

Multiplex PCR was positive in 96% of isolates and 4 isolates had negative results. Falsepositive
results were reported with three E. coli strains. Multiplex PCR identified all the isolates of
Acinetobacter baumannii, 29 isolates of Pseudomonas aeruginosa and 27 isolates of Stenotrophomonas
maltophilia. The diagnostic value of the multiplex PCR compared to the biochemical identification
revealed sensitivity 96.04%, specificity 96.9%, positive predictive value 97.00%, negative
predictive value 96.00% and accuracy 96.50%.

Conclusion:

The present study highlights the diagnostic value of multiplex PCR to identify Acinetobacter
baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive
blood cultures. Multiplex PCR was sensitive, specific and accurate. The accuracy differs according
to the organisms with 100% accuracy for Acinetobacter baumannii.
Organocatalysis: An Overview of its Application in Oxidation and Reduction
Reactions
Authors: Mukhopadhyay Chhanda , Pal Rammyani
Published: July 2021

:

Organocatalysis was established to be a wide-applicable approach from its inception and
rediscovery in the year 2000, where proline was used as a catalyst in aldol condensation and soon
after the successful emergence of iminium catalysed reactions in organic synthesis. Development
of new potential catalytic systems is always an important as well as an uphill task for scientists and
researchers. The fundamental organic synthesis majorly deals with metal based catalysts, whereas
there is a constant surge of developing metal free reaction condition to make the reactions environmental
friendly. For the synthesis of complex organic molecules, reduction and oxidation reactions
are always needed and there are plenty of catalysts available for these reactions. Organocatalysts
are also developed and applied for these two elementary reactions. This review focuses on some of
the latest developments and applications of organocatalysts in oxidation and reduction reactions in
fundamental organic synthesis.
Desafíos de la educación superior para la gestión de la calidad
Authors: González Rodríguez Nadia Aurora , Muñoz Verdezoto Patricia Jacqueline , Castañeda Paredes César , Moya Jordán Karyna , Portilla Castell Yoenia , Bell Rodríguez Rafael Félix , De la Llana Pérez Evelyn , Pinargote Cedeño Walter Wladimir , Marrero Sánchez Odalys , Mohamed Amar Rachida , Xifra Triadú Jordi , Hidalgo León José Alonso , Chiriboga Cisneros Edison Fernando , Baquerizo Álava Verónica
Published: July 2021

La gestión en los procesos educativos constituye un desafío para la Educación Superior en los actuales momentos. Uno de los aspectos de vital importancia es contribuir a un mejor desempeño ante los cambios globales que constantemente impone las nuevas necesidades del desarrollo social del siglo XXI. Una educación de calidad promueve la igualdad de oportunidades de todos los ciudadanos de un país, para que estos puedan adquirir las competencias básicas en los primeros niveles de enseñanza. Por esta razón, para cumplir con esta meta es importante la presencia de un adecuado liderazgo que guíe los procesos educativos tomando en consideración los recursos reales de su práctica profesional, así como de su contexto situacional. Muchos son los factores que pueden abordarse en relación con lo antes mencionado, sin embargo, el pilar indispensable para una correcta administración de la educación lo constituye la manera en que se diagnostican, organizan, implementan y evalúan las acciones con el fin de lograr los objetivos propuestos por el enter regulador correspondiente al país. En este sentido, los docentes de los diferentes niveles educativos deben velar porque estas directrices se cumplan, además de adecuarlos a la impronta particular que identifica a su institución. En el caso de la Enseñanza Superior, la relevancia radica en la mejora continua, es decir, seguir fortaleciendo habilidades que debieron ser adquiridas en los niveles anteriores con el compromiso de asumir los obstáculos que en ocasiones se evidencian debido a las insuficiencias que se pueden presentar tanto en las estrategias didáctica como la experticia de los docentes. Esta compilación incluye las siete mejores investigaciones presentadas en el Área del conocimiento Gestión de los Procesos de la educación Superior del lll Congreso Sociedad del Conocimiento: Retos y Perspectivas de la Universidad Tecnológica ECOTEC.
La educación superior y las TIC
Authors: Noa Silverio Luisa A. , Cañizares González Roxana , Salvador Broche Orlando F. , Estrada Sentí Vivian , Febles Rodríguez Juan P. , Granda Dihigo Ailec , Hernández Aguilar Violena , Fernández Pérez Yamilis , Santos Ramírez Yunier , González Hazim Ninoshka , Martínez Leye Olga Lidia , Serrano Pérez Walfrido , Mompié Fuente Iyatne , Blanco Hernández Sahara M. , Burguet Lago Ivonne , Fuentes Betancourt Juan , Pérez Perdomo Armando , Calzadilla Amaya Octavio , Ezquerra Quintana Geraldine , Gil Mateos Jorge E. , Ramírez Píre Manuel Osmany , Medina Moreira José J. , Núñez Jover Jorge , Armas Marrero Isvieysys , Martín Lloró Víctor Manuel , Patterson Peña Marisol , Maciá Gravier María Elena , Arellano Arcentales Ana María
Published: July 2021

El presente libro que se pone a disposición del lector, es una colección de artículos de autores cubanos y ecuatorianos sobre sus experiencias e investigaciones en la Educación Superior. En los mismos se reflejan diferentes consideraciones sobre el uso de las tecnologías y de los entornos virtuales de aprendizaje generados por ellas. Es posible preguntar si el lector ha desarrollado algún curso en línea o si se ha visto involucrado en la organización y gestión de los mismos. Si es así, seguramente ha podido comprobar las posibilidades que brindan las tecnologías de la comunicación y la información para el proceso de enseñanza - aprendizaje tanto para los modelos educativos completamente a distancia o en línea, como aquellos mixtos que complementan la presencialidad con el uso de estas tecnologías. Hoy en día, es de aceptación general que estos modelos convergen y que sus fronteras, cada vez más, se hacen confusas. El aprendizaje en línea, en numerosos países, forma parte ya del ambiente educativo y es una extensión más del campus universitario.
La universidad nueva : Fundamentos desde los procesos sustantivos
Authors: Alfonso Caveda Duniesky , Marrero Sánchez Odalys , Muñoz Verdezoto Patricia Jacqueline , Castañeda Paredes César Jacinto , Alcívar Trejo Carlos Esteban , González Rodríguez Nadia Aurora , Calderón Farfán Elba Elizabeth
Published: July 2021

El fenómeno educativo como fenómeno sociocultural complejo constituye el resumen de una época, sus características y aspiraciones. Tiene el privilegio de evidenciar como ningún otro los aciertos y desaciertos en la formación de sus ciudadanos. La formación universitaria, como integrante de este, debe cumplir entonces con el conocido pero difícil objeto de satisfacer las necesidades profesionales de toda sociedad. La Universidad ECOTEC, comprende este principio y toda su labor, desde su fundación, ha estado encaminado a cumplir este fin. La integración de los procesos sustantivos y su adecuada gestión constituyen el necesario camino para una formación integral, contextualizada y pertinente. Las nuevas transformaciones educativas a nivel internacional, regional y ecuatoriana en el ámbito de los procesos de acreditación y rediseño de la oferta académica serían estériles sin una clara visión docente metodológica y pedagógica que sirviera de columna vertebral al desarrollo de sus procesos sustantivos. Por esta razón, la presente compilación es, en buena medida, el resumen de lo anterior en la siempre ardua labor de cambiar mentes y romper paradigmas.
Manufacturing employment in the Southeast: examining the last 30 years
Authors: Zhang Wendy
Published: July 2021

U.S. manufacturing has been in decline for the last forty years, bringing real economic impact. This paper examines the Southern region experience. Variation in rate of decline and industry evolution are shown across states. The important transportation equipment manufacturing industry is explored in more detail. The overall takeaway is that manufacturing looks different across the Southeast.