bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling Software Limitations in the Assessment of the Minimum Sectional Area and Volume in Cleft LIP and Palate Patients
Authors: Costa Beethoven Estevao , Yaedú Renato Yassutaka Faria , Pereira-Silva Maísa , Fabris André Luis da Silva , Garcia-Usó Michele , Magro Filho Osvaldo , Soares Simone
Published: February 2025

The increasing use of cone beam computed tomography (CBCT) has led to a growing demand for DICOM software that enables the assessment and measurement of craniofacial structures. This study aimed to compare the airway volume and the minimum axial area in patients with cleft lip and palate using five different imaging software programs: Dolphin3D, InVivo Dental, ITK Snap, InVesalius, and NemoFAB. Initially, 100 CBCT scans were selected by an examiner, and their corresponding DICOM files were collected. The oropharyngeal segments were delineated following the manufacturer’s guidelines, using two different segmentation techniques: interactive and fixed threshold. The results were analyzed using the Friedman test and Wilcoxon post hoc test, with a 5% significance level for all statistical tests. The findings for both the minimum axial area and total volume revealed that the median values across the software groups were higher than expected, and significant differences were observed when comparing the groups (p < 0.001). All five software programs showed notable differences in their outputs. Specifically, a statistically significant difference in volume was found across all groups, except between InVivo and ITK-Snap. It is recommended that pre- and post-treatment comparisons be performed using the same software for consistency.
Friction in Cylindrical Joints
Authors: Mihalache Andrei Marius , Merticaru Vasile , Ermolai Vasile , Dodun Oana , Nagiț Gheorghe , Hrițuc Adelina , Rîpanu Marius Ionuț , Slătineanu Laurențiu
Published: February 2025

Cylindrical clearance joints are commonly employed in mechanisms that involve the rotation of a shaft spindle within a cylindrical sliding bearing. The intensity of the friction process in such joints is governed by several factors, including the clearance size between components, the materials of the interacting surfaces, the properties and characteristics of the lubricant, the surface roughness (asperities), and the magnitude of the relative velocity between the joint’s components. To experimentally determine the friction coefficient in cylindrical clearance joints, a custom device was designed and implemented. This device is adaptable to a universal lathe and enables the measurement of the friction coefficient under varying normal forces and relative movement speeds between the joint components. The experimental data were subjected to mathematical analysis, leading to the development of an empirical model. This model effectively characterizes the direction and intensity of the influence of various factors on the friction coefficient, accounting for the use of different lubricants. The findings provide valuable insights into optimizing cylindrical clearance joints for improved performance in practical applications.
Synthesis and Characterization of Metakaolin–Wollastonite Geopolymer Foams for Removal of Heavy Metal Ions from Water
Authors: Alshaaer Mazen , Alharbi Bader , Alqahtani Obaid , Alotaibi Mohammed S. , Alzayed Abdullah , Al-Kafawein Juma’a
Published: February 2025

Over the past few decades, researchers have focused on developing new compositions and preparation techniques for geopolymers, as multifunctional products, to optimize their characteristics for use in multiple applications. Therefore, this paper investigates metakaolin geopolymer foam and introduces new geopolymer foams based on hybrid metakaolin and wollastonite mineral precursors for water purification. The geopolymer foams were prepared using an alkaline activator, mineral-based powders (wollastonite and metakaolin), a foaming agent (aluminum powder), and a foam stabilizer (olive oil). In addition to mechanical tests and assessments of the adsorption capacity of heavy metal ions, the geopolymer foams were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). The geopolymer foams exhibited unique pore structures, containing four classes of pore networks with diameters around 1000 µm, 25 µm, 3 µm, and a well-arranged mesopore network of 50 nm. The utilization of wollastonite (CaSiO3) alongside metakaolin as a hybrid precursor led to fundamental changes in the composition of the geopolymer binders: a new crystal phase, Ca5(SiO4)2(OH)2, was formed, and the Si-Al-Na crystal phase disappeared, which led to an increase in the amorphous phase from 87% to 92%. The adsorption rate of heavy metal ions, namely Cr, Co, Cu, Zn, Pd, and As, increased upon introducing wollastonite as a precursor, with absorption rates ranging from 11% to 68%. The findings also revealed that wollastonite significantly increased the geopolymers foams’ compressive strength and elastic modulus from 30 KPa to 67 KPa and from 31 MPa to 126 MPa, respectively.
Laser-Micro-Annealing of Microcrystalline Ni-Rich NCM Oxide: Towards Micro-Cathodes Integrated on Polyethylene Terephthalate Flexible Substrates
Authors: Ahrens Lara , Mikulics Martin , Schröder Steffen , Mayer Joachim , Hardtdegen Hilde Helen
Published: February 2025

Here in this work, we report on micro-Raman spectroscopy investigations performed on freestanding Ni-rich NCM (LixNi0.83Co0.11Mn0.06O2) microcrystals transferred to flexible polyethylene terephthalate (PET) host substrates. This technological procedure introduces a first building block for future on-chip-integrated micro-accumulators for applications in flexible optoelectronics, sensors, microbiology, and human medicine. An after-synthesis thermal treatment was used to help improve the material homogeneity and perfection of the cathode material. To this end, a local laser micro-annealing process was applied to the freestanding Ni-rich NCM microcrystals. The thermally initialized structural processes in the singular micro-cathode units were characterized and determined by micro-Raman spectroscopy. Micro-Raman mapping images revealed the evolution of a recrystallization process after the local annealing procedure. Furthermore, laser micro-annealing led to the suppression of the pristine “polycrystalline morphology” of the investigated micro-cathode regions. Besides the dominant characteristic Raman mode at ~1085 cm−1, most likely ascribed to lithium carbonate, metal oxides with Raman modes around ~550 cm−1 were identified. This highly efficient transfer and integration technology represents a basic building block towards micrometer-sized accumulators for a large range of emerging applications.
Geopolymers Manufactured by the Alkali Activation of Mining and Ceramic Wastes Using a Potential Sustainable Activator from Olive Stone Bottom Ashes
Authors: Carrillo Beltran Raul , Picazo Camilo Elena , Perea Toledo Griselda , Corpas Iglesias Francisco Antonio
Published: February 2025

The reuse of by-products as alternative raw materials to traditional construction materials is required in order to ensure sustainable development in the construction sector and is a significant and important focus in the fields of materials science. This study developed geopolymers using by-products from mining, ceramics, and olive industries, including slate stone cutting sludge (SSCS) and chamotte (CH) as aluminosilicate sources, and olive biomass bottom ash (OSBA) as an alkaline activator with sodium silicate. A key novelty of the research lies in the use of SSCS, an underexplored by-product in geopolymerization studies, as a viable aluminosilicate source. The geopolymers were prepared with varying weight ratios of SSCS, CH, and OSBA/Na₂SiO₃ (1.7, 1.9, 2.2, and 2.4). Physical and mechanical tests determined the optimal formulation, while FTIR and SEM analyses revealed the material’s chemical and structural evolution. The FTIR analysis detected the quartz and carbonate phases, indicating incomplete quartz dissolution and carbonate formation during calcination. The SEM analysis revealed a dense microstructure with reduced porosity and enhanced geopolymerization in samples with higher OSBA content. The optimal geopolymer (60% OSBA, 30% CH, OSBA/Na₂SiO₃ ratio of 2.2) achieved a compressive strength of 33.1 MPa after 28 days. These findings demonstrate the feasibility of producing geopolymers using SSCS, CH, and OSBA, promoting the reuse of industrial by-products as sustainable alternatives to conventional binders.
Improved Confidence Intervals for Expectiles
Authors: Penev Spiridon , Maesono Yoshihiko
Published: February 2025

Expectiles were introduced to statistics around 40 years ago, but have recently gained renewed interest due to their relevance in financial risk management. In particular, the 2007–2009 global financial crisis highlighted the need for more robust risk evaluation tools, leading to the adoption of inference methods for expectiles. While first-order asymptotic inference results for expectiles are well established, higher-order asymptotic results remain underdeveloped. This study aims to fill that gap by deriving higher-order asymptotic results for expectiles, ultimately improving the accuracy of confidence intervals. The paper outlines the derivation of the Edgeworth expansion for both the standardized and studentized versions of the kernel-based estimator of the expectile, using large deviation results on U-statistics. The expansion is then inverted to construct more precise confidence intervals for the expectile. These theoretical results were applied to moderate sample sizes ranging from 20 to 200. To demonstrate the advantages of this methodology, an example from risk management is presented. The enhanced confidence intervals consistently outperformed those based on the first-order normal approximation. The methodology introduced in this paper can also be extended to other contexts.
Internality of Two-Measure-Based Generalized Gauss Quadrature Rules for Modified Chebyshev Measures II
Authors: Djukić Dušan Lj. , Mutavdžić Djukić Rada M. , Pejčev Aleksandar V. , Reichel Lothar , Spalević Miodrag M. , Spalević Stefan M.
Published: February 2025

Gaussian quadrature rules are commonly used to approximate integrals with respect to a non-negative measure dσ^. It is important to be able to estimate the quadrature error in the Gaussian rule used. A common approach to estimating this error is to evaluate another quadrature rule that has more nodes and higher algebraic degree of precision than the Gaussian rule, and use the difference between this rule and the Gaussian rule as an estimate for the error in the latter. This paper considers the situation when dσ^ is a Chebyshev measure that is modified by a linear factor and a linear divisor, and investigates whether the rules in a recently proposed new class of quadrature rules for estimating the error in Gaussian rules are internal, i.e., if all nodes of the new quadrature rules are in the interval (−1,1). These new rules are defined by two measures, one of which is a modified Chebyshev measure dσ^. The other measure is auxiliary.
Safety and Efficacy of Echo- vs. Fluoroscopy-Guided Pericardiocentesis in Cardiac Tamponade
Authors: Simeunović Dejan S. , Milinković Ivan , Polovina Marija , Trifunović Zamaklar Danijela , Veljić Ivana , Zaharijev Stefan , Babić Marija , Nikolić Dejan , Perić Valerija , Gatarić Nina , Ristić Arsen D. , Seferović Petar M.
Published: February 2025

Background and Objectives: Cardiac tamponade is managed through echo- or fluoroscopy-guided percutaneous pericardiocentesis. The European Society of Cardiology’s Working Group on Myocardial and Pericardial Diseases proposed a triage strategy for these patients. This study evaluated the triage score and compared the safety and efficacy of fluoroscopy- versus echo-guided procedures without additional visualization control. Materials and Methods: This prospective observational study included 71 patients with cardiac tamponade from February 2021 to June 2022. Pericardiocentesis was performed using fluoroscopy or echo guidance based on clinical assessment and catheterization laboratory availability, without the additional control of needle/guidewire position or ECG monitoring. Patients were followed for three months. Results: The study included 71 patients (52.1% female, mean age 59.7 ± 15.7 years). Malignancy was the most common comorbidity (59.2%). Echo criteria led to urgent procedures in 47.9%, with subcostal access used most often (60.6%), particularly in fluoroscopy-guided procedures (93.8%, p = 0.003). The success rate was 97.1%, with minor complications in 14% of patients. Diabetes and malignancy predicted complications regardless of access site or guiding method. The triage score did not affect complication rates or short-term mortality. Conclusions: Fluoroscopy- and echo-guided pericardiocentesis without additional visualization control showed no difference in safety or efficacy. Delaying the procedure for patients with a triage score ≥6, or performing it early for those with a low score, did not impact complication rates or mortality, which were more influenced by the progression of the underlying disease.
Optimised Skeletal Muscle Mass as a Key Strategy for Obesity Management
Authors: Barber Thomas M. , Kabisch Stefan , Pfeiffer Andreas F. H. , Weickert Martin O.
Published: February 2025

The ‘Body Mass Index’ (BMI) is an anachronistic and outdated ratio that is used as an internationally accepted diagnostic criterion for obesity, and to prioritise, stratify, and outcome-assess its management options. On an individual level, the BMI has the potential to mislead, including inaccuracies in cardiovascular risk assessment. Furthermore, the BMI places excessive emphasis on a reduction in overall body weight (rather than optimised body composition) and contributes towards a misunderstanding of the quiddity of obesity and a dispassionate societal perspective and response to the global obesity problem. The overall objective of this review is to provide an overview of obesity that transitions away from the BMI and towards a novel vista: viewing obesity from the perspective of the skeletal muscle (SM). We resurrect the SM as a tissue hidden in plain sight and provide an overview of the key role that the SM plays in influencing metabolic health and efficiency. We discuss the complex interlinks between the SM and the adipose tissue (AT) through key myokines and adipokines, and argue that rather than two separate tissues, the SM and AT should be considered as a single entity: the ‘Adipo–Muscle Axis’. We discuss the vicious circle of sarcopenic obesity, in which aging- and obesity-related decline in SM mass contributes to a worsened metabolic status and insulin resistance, which in turn further compounds SM mass and function. We provide an overview of the approaches that can mitigate against the decline in SM mass in the context of negative energy balance, including the optimisation of dietary protein intake and resistance physical exercises, and of novel molecules in development that target the SM, which will play an important role in the future management of obesity. Finally, we argue that the Adipo–Muscle Ratio (AMR) would provide a more clinically meaningful descriptor and definition of obesity than the BMI and would help to shift our focus regarding its effective management away from merely inducing weight loss and towards optimising the AMR with proper attention to the maintenance and augmentation of SM mass and function.
Clustering-Based Identification of BMI-Associated Metabolites with Mechanistic Insights from Network Analysis in Korean Men
Authors: Park JooYong , Kang Jihyun , Lee Ji-Yeoun , Kang Daehee , Cho Joo-Youn , Choi Ji-Yeob
Published: February 2025

Background: Epidemiological studies using metabolomics often encounter challenges due to metabolite profiles being influenced by multiple modifiable behavioral factors, including regular exercise, smoking, drinking, and weight control. This study aimed to identify modifiable behavioral factors reflected in metabolites by clustering subjects based on their metabolite profiles. Networks of metabolites were constructed to visualize their relationships and the differences between clustering groups. Methods: Sixty-four healthy men were included in this study. Information on regular exercise, smoking, and drinking was collected by questionnaires, and body mass index (BMI), an indicator of weight control, was calculated based on measured height and weight. Through targeted metabolomics, the concentrations of 149 metabolites were quantified. Subjects were clustered using the k-means method based on metabolite composition. Correlation-based networks were constructed for each cluster using Cytoscape software, followed by network analysis. Results: The subjects were divided into two clusters, with BMI identified as a distinguishing feature. Four lyso-phosphatidylcholines (PCs), six diacyl-PCs, and one acyl-alkyl-PC were positively associated with BMI. In the constructed network, acyl-alkyl-PCs exhibited the highest degrees, suggesting their central role in BMI-associated metabolic pathways. Conclusions: These findings suggest that metabolites can reflect behavioral factors, with BMI exerting a significant influence on metabolite profiles, particularly through its associations with phosphatidylcholines.
Metabolic Syndrome Spectrum in Children with Classic Congenital Adrenal Hyperplasia—A Comprehensive Review
Authors: Panic Zaric Sanja , Milenkovic Tatjana , Todorovic Sladjana , Mitrovic Katarina , Cvetkovic Dimitrije , Cehic Maja , Vekic Jelena , Dumic Katja , Vukovic Rade
Published: February 2025

Children with a classic form of congenital adrenal hyperplasia (CCAH) have a potentially increased risk of unfavorable cardiometabolic events due to the interplay of corticosteroid treatment, hyperandrogenism, and other factors. Although readily recognized in adults, these aspects are frequently overlooked in children and youth with CCAH; Aim: To review the evidence available from studies regarding cardiometabolic health outcomes in CCAH patients; Methods: A review of the literature was performed following PRISMA guidelines, including studies published between 2000 and 2024. We included studies reporting cardiometabolic outcomes in children and adolescents (<18 years) with CCAH. Where pediatric data were sparse, additional data were obtained from studies with older adolescents and young adults (15–25 years). Cardiometabolic outcomes included risk factors, such as obesity, insulin resistance, lipids, blood pressure, and vascular markers; Results: Twenty-five studies were analyzed. The prevalence of obesity was found to be higher in children with CCAH, as well as of increased visceral adiposity. Higher indices of insulin resistance were also a frequent finding in children with CCAH. CCAH patients had higher systolic blood pressure and more frequently loss of nocturnal blood pressure dipping, particularly among salt-wasting subtypes and in younger children. Subclinical atherosclerosis was indicated by increased carotid intima–media thickness, elevated hs-CRP, and impaired endothelial function. Other findings suggested changes in lipid profiles, particularly decreased HDL-c and increased triglycerides, although the findings were less consistent; Conclusions: Compared with the general pediatric population, children with CCAH were found to have an increase in multiple cardiometabolic risk factors. It is therefore vital to monitor these risk factors in pediatric CCAH, as well as tailoring treatment with cardiometabolic health in mind, to achieve better long-term cardiovascular and metabolic outcomes. Future research should focus on longitudinal studies of cardiometabolic outcomes and innovative therapeutic approaches to reduce these risks in patients with CCAH.
In-Depth Investigation on Potential Mechanism of Forest-Grown Ginseng Alleviating Alzheimer’s Disease via UHPLC-MS-Based Metabolomics
Authors: Niu Huazhou , Zhang Meng , Zhang Kaiyue , Aishan Saibire , Li Hui , Wu Wei
Published: February 2025

Background: Alzheimer’s disease is a central nervous system degenerative disease closely related to age with a complex pathogenesis. As a natural medicinal plant, forest-grown ginseng (GSF) contains abundant ginsenosides and offers significant neuroprotective effects. Methods: In this study, we comprehensively investigated the effect of GSF on the cell viability of PC12 cells in an AD model alongside metabolic changes in the serum and brains of mice, combined with an efficacy evaluation of PC12 cells in vitro and UHPLC-MS-based metabolomics in vivo. The goal of this study is to clarify the potential mechanism of GSF in treating AD. Results: The PC12 cell results showed that GSF can promote the proliferation of PC12 cells, reduce the content of IL-8, increase the activity of SOD, and alleviate the inflammation and oxidative stress induced by Aβ25~35. The immunohistochemical results for the mouse brain tissue also showed that GSF could reduce the inflammatory response of mouse brain tissue by reducing the overexpression of IBa1. AD was alleviated by reducing Aβ protein deposition in the mouse brain tissue. An untargeted metabolomics analysis was performed using UHPLC-Q-Exactive MS and principal component analysis (PCA) to identify the differentially expressed metabolites in the serum and brain tissue of AD mice after treatment. Twenty and seventeen different metabolites were identified in the serum and brain tissue, respectively. The pathway enrichment analysis of differential metabolites showed that GSF could treat AD by up-regulating succinic acid semialdehyde, carbamoyl phosphate, Sphingosine 1-phosphate, L-cystathionine, 2-ketobutyric acid, Vanillylmandelic acid, and D-Ribose to regulate sphingomyelin metabolism, the synthesis and metabolism of neurotransmitters and precursors, and energy metabolism. Conclusions: GSF can reduce neuroinflammation and alleviate Alzheimer’s disease by regulating the metabolic disorders of amino acids, sphingolipids, unsaturated fatty acids, and arachidonic acid in mice serum and brain tissue metabolites. These results suggest a link between metabolite imbalance and AD, and reveal the basis for the mechanism of ginsenosides in AD treatment.
Metabolites and Free Fatty Acids in Japanese Black Beef During Wet Aging
Authors: Ueda Shuji , Yoshida Yuka , Tateoka Yuka , Kebede Biniam , Shinohara Masakazu , Nakanishi Hiroki , Fukuda Itsuko , Shirai Yasuhito
Published: February 2025

Background: Japanese Black beef is known for its high intramuscular fat content, an important factor in its distinctive Wagyu aroma. Wet aging, which involves vacuum-packing meat and storing it at low temperatures, enhances flavor, texture, and tenderness and is essential for maintaining and improving meat quality. In this study, changes in metabolites and lipid profiles were investigated during the wet aging of Japanese Black and Holstein beef. Methods/Results: Gas chromatography–mass spectrometry identified 113 metabolites in Japanese Black beef and 94 in Holstein beef, with significant increases in metabolites like aspartic acid and maleic acid over the aging period. Regarding lipid composition, total free fatty acids significantly increased with wet aging, with Japanese Black beef showing significantly higher concentrations of oleic and linoleic acids than Holstein beef. Additionally, lipid analysis by liquid chromatography–mass spectrometry revealed a reduction in specific phospholipids, particularly lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), with notable decreases in LPC (18:1), LPC (18:2), LPE (18:1), and LPE (18:2). Conclusions: These results suggest that wet aging influences the stability of membrane lipids, facilitating the degradation of phospholipids into free fatty acids, and improving the flavor of Japanese Black beef.
A Novel Genotype of Orientia tsutsugamushi in Human Cases of Scrub Typhus from Southeastern India
Authors: Nallan Krishnamoorthy , Kalidoss Bhuvaneshwari Chinnathambi , Jacob Eunice Swarna , Mahadevan Samyuktha Krishnasamy , Joseph Steny , Ramalingam Ramkumar , Renu Govindarajan , Thirupathi Balaji , Ramasamy Balajinathan , Gupta Bhavna , Rahi Manju , Rajaiah Paramasivan
Published: February 2025

Scrub typhus is a mite-borne, re-emerging public health problem in India, particularly in Tamil Nadu, South India. More than 40 serotypes of Orientia tsutsugamushi have been documented worldwide. However, the information on the circulation of its molecular sub-types in India is scanty. A retrospective study was conducted among serologically confirmed cases of scrub typhus. DNA isolated from blood was screened by a nested polymerase chain reaction (nPCR) targeting the GroEL and the 56 kDa type-specific antigen (TSA) genes. Out of 59 samples, 14 partial fragments of GroEL and the twelve 56 kDa genes were PCR-amplified and DNA-sequenced. The neighbor-joining (NJ) analysis indicated three distinct phylogenetic clades, including a novel genotype designated as Ot-Thanjavur-Tamil Nadu (Ot-TJTN, 9 nos. 64.3%); Karp-like (4 nos. 28.6%); and Kuroki-Gilliam type (1 no. 7.1%). Also, phylogenetic analysis of twelve 56 kDa variable domains (VDΙ-ΙΙΙ) of TSA gene sequences revealed a distinctive new genotypic cluster of eight samples (66.6%), and the remaining four (33.4%) were Karp-like genotypes. The Simplot analysis for the similarity and event of recombination testing elucidated the existence of the new genotype of the Ot-TJTN cluster, which was undescribed so far, in the Kato and TA716 lineages. The significant findings recommend further studies to understand the ongoing transmission dynamics of different O. tsutsugamushi strains in vector mites, rodent hosts, and humans in this region.
Comprehensive Methodology for Evaluating the Drug Loading of Iron Oxide Nanoparticles Using Combined Magnetometry and Mössbauer Spectroscopy
Authors: Iacob Nicusor , Palade Petru , Comanescu Cezar , Crisan Ovidiu , Toderascu Luiza Izabela , Socol Gabriel , Schinteie Gabriel , Kuncser Victor
Published: February 2025

A methodology for the quantitative estimation of the drug loading of iron oxide-based magnetic nanoparticles by corroborating magnetometry and Mössbauer spectroscopy investigations is reported. The proposed methodology is exemplified in the case of two series of nanoparticles, namely Fe3O4 nanoparticles covered with citric acid molecules and further functionalized with doxorubicin, and Fe3O4 nanoparticles covered with L-Cysteine molecules and further functionalized with doxorubicin. The general idea of the proposed methodology is to probe the real magnetic structure of the magnetic core via low-temperature Mössbauer spectroscopy for the correct estimation of the spontaneous magnetization of the magnetic core. It subsequently uses the ratio between the spontaneous magnetization of the covered nanoparticles and that of the magnetic core for the reliable and nondestructive evaluation of the nanoparticle loading by organic molecules. Although the methodology is exemplified in the case of magnetite-based nanoparticles, it can be successfully considered for a large class of medicine-loaded Fe-containing magnetic nanoparticles where 57Fe Mössbauer spectroscopy can be applied.
Rigid Polyurethane Foam Derived from Renewable Sources: Research Progress, Property Enhancement, and Future Prospects
Authors: Yuan Yao , Guo Qinhe , Xu Lulu , Wang Wei
Published: February 2025

Rigid polyurethane foam (RPUF) is a widely utilized thermosetting polymer across various industrial applications, valued for its exceptional properties. However, the demand for sustainable alternatives to petroleum-based polymers has grown increasingly urgent due to rising environmental concerns. Despite its widespread use, RPUF faces challenges such as inadequate mechanical strength, limited thermal stability, and high flammability, all of which are crucial considerations in commercial and household applications. Globally, ongoing efforts are focused on developing innovative technologies that convert renewable sources into new monomers and polymers, some of which could serve as alternatives to traditional RPUFs. Several approaches have been explored to improve the thermal stability, mechanical strength, and flame retardancy of RPUFs, including the modification of bio-based polyols and the incorporation of performance-enhancing fillers. This review emphasizes recent advances in RPUFs derived from natural resources, focusing on their preparation, characterization, and properties, and strategies to enhance the mechanical strength and flame safety of bio-based RPUFs. Additionally, it explores the applications of RPUF materials across various fields, addressing the challenges and potential developments in packaging, household items, construction, and automotive applications.
Biosorption of Cr (III) from Polluted Water Using Pennisetum clandestinum Hochst (Kikuyo)
Authors: Maldonado-Farfán Amanda Rosa , Fernández-Bernaola Uriel Raul , Vargas-Robles Rocio Victory , Villasante-Muñoz Jessica Gabriela , Trejo-Valdez Martin Daniel
Published: February 2025

Given the abundance of kikuyu biomass resulting from the pruning of green areas, the aim of this study was to evaluate its use as a biosorbent (BK) for Cr (III) removal from polluted waters. The biomass was activated using H2SO4 (1.25%) and NaOH (3.25%). The characterization methods were Fourier-Transform Infrared Spectroscopy (FTIR), scanning electron microscopy/energy-dispersive spectroscopy (SEM/EDS), and Brunauer–Emmett–Teller (BET) analysis. Our results confirmed the presence of active groups on BK, such as –OH, -C=C-, -C=O, and -C-O-, with an increase of 1308.58% in specific surface area, as well as the presence of chromium on the biosorbent after adsorption process. The adsorption capacity (q) was tested in a jar test as a function of biomass granulometry, dose (BK), and the pH of the solution; the best response was 47.9 mg/g at a pH of 5.5, a biosorbent dose of 0.5 g/L, and a biosorbent size of 100 μm. The effect of pH was positive; by increasing the pH, the adsorption capacity increased. However, the effect of the biosorbent dose and size was negative, as when increasing the dose and granulometry, the adsorption capacity decreased. In addition, the kinetic process was studied, where the removal data were better fitted for the pseudo-second-order kinetic model, confirming that the adsorption mechanism was chemisorption. The adsorption capacity was 37.6 mg/g for industrial wastewater. The possibility of using kikuyu within the circular economy was demonstrated and suggests its application in continuous systems for real-world environmental conditions.
Chemical Composition of Essential Oils and Their Potential Applications in Postharvest Storage of Cereal Grains
Authors: Yu Jianmei
Published: February 2025

Insect infestation and microbial, particularly mold contamination, are the major causes of stored grain deterioration during postharvest storage, which results in a significant loss in grain quality and quantity, and the formation of toxic chemicals such as mycotoxins. Pesticides, together with physical protection strategies, have been widely used to control insects and molds in stored grains, but their uses present significant environmental and health problems. This has led to the exploration of safer pesticide alternatives. Essential oils (EOs) are highly concentrated materials extracted from leaves, stems, flowers, seeds, roots, fruit rinds, resins, or barks. They are multifunctional due to their complex chemical composition. Thus, EOs are frequently used for their therapeutic, antimicrobial, odoriferous, and flavor properties in a wide range of products like medicine, cosmetics, and foods. This review provides comprehensive information on the chemical compositions of EOs commonly used in the food industry, factors influencing EO composition, and recent studies on the potential of EOs as alternatives to synthetic pesticides and fungicides for stored grain protection. The relationship between chemical compositions of EOs and their anti-insects and antimicrobial potentials, as well as current approaches/technologies of using EOs for food preservation, are also covered. However, this review also highlights the need for research on the development of feasible and affordable methodologies to apply effective EOs or encapsulated EOs in grain storage settings, particularly for organic grain protection.
New Perspectives of Hydrogels in Chronic Wound Management
Authors: Alberts Adina , Bratu Andreea Gabriela , Niculescu Adelina-Gabriela , Grumezescu Alexandru Mihai
Published: February 2025

Chronic wounds pose a substantial healthcare concern due to their prevalence and cost burden. This paper presents a detailed overview of chronic wounds and emphasizes the critical need for novel therapeutic solutions. The pathophysiology of wound healing is discussed, including the healing stages and the factors contributing to chronicity. The focus is on diverse types of chronic wounds, such as diabetic foot necrosis, pressure ulcers, and venous leg ulcers, highlighting their etiology, consequences, and the therapeutic issues they provide. Further, modern wound care solutions, particularly hydrogels, are highlighted for tackling the challenges of chronic wound management. Hydrogels are characterized as multipurpose materials that possess vital characteristics like the capacity to retain moisture, biocompatibility, and the incorporation of active drugs. Hydrogels’ effectiveness in therapeutic applications is demonstrated by how they support healing, including preserving ideal moisture levels, promoting cellular migration, and possessing antibacterial properties. Thus, this paper presents hydrogel technology’s latest developments, emphasizing drug-loaded and stimuli-responsive types and underscoring how these advanced formulations greatly improve therapy outcomes by enabling dynamic and focused reactions to the wound environment. Future directions for hydrogel research promote the development of customized hydrogel treatments and the incorporation of digital health tools to improve the treatment of chronic wounds.
Aluminum–Silica Core–Shell Nanoparticles via Nonthermal Plasma Synthesis
Authors: Cameron Thomas , Klause Bailey , Loh Kristine Q. , Kortshagen Uwe R.
Published: February 2025

Aluminum nanoparticles (Al NPs) are interesting for energetic and plasmonic applications due to their enhanced size-dependent properties. Passivating the surface of these particles is necessary to avoid forming a native oxide layer, which can degrade energetic and optical characteristics. This work utilized a radiofrequency (RF)-driven capacitively coupled argon/hydrogen plasma to form surface-modified Al NPs from aluminum trichloride (AlCl3) vapor and 5% silane in argon (dilute SiH4). Varying the power and dilute SiH4 flow rate in the afterglow of the plasma led to the formation of varying nanoparticle morphologies: Al–SiO2 core–shell, Si–Al2O3 core–shell, and Al–Si Janus particles. Scanning transmission electron microscopy with a high-angle annular dark-field detector (STEM-HAADF) and energy-dispersive X-ray spectroscopy (EDS) were employed for characterization. The surfaces of the nanoparticles and sample composition were characterized and found to be sensitive to changes in RF power input and dilute SiH4 flow rate. This work demonstrates a tunable range of Al–SiO2 core–shell nanoparticles where the Al-to-Si ratio could be varied by changing the plasma parameters. Thermal analysis measurements performed on plasma-synthesized Al, crystalline Si, and Al–SiO2 samples are compared to those from a commercially available 80 nm Al nanopowder. Core–shell particles exhibit an increase in oxidation temperature from 535 °C for Al to 585 °C for Al–SiO2. This all-gas-phase synthesis approach offers a simple preparation method to produce high-purity heterostructured Al NPs.
A Rapid and Surfactant-Free Synthesis Strategy for Variously Faceted Cuprous Oxide Polyhedra
Authors: Liu Kaihao , Xin Yu , Gao Shikun , Yu Yadong , Dai Mengyan , Liu Zhe
Published: February 2025

We systematically investigated the morphology-controlled synthesis of Cu2O micro-nano crystals, especially under surfactant-free conditions, targeting a simple, rapid, and morphologically controllable preparation strategy for polyhedral Cu2O micro-nano crystals. By systematically investigating the effects of NaOH concentration, types of reducing agents, and copper salt precursors on crystal growth, precise control over the morphology of Cu2O crystals under surfactant-free conditions was achieved. This method can rapidly prepare variously faceted Cu2O crystals under mild conditions (70 °C, 7 min), including regular polyhedra with low-index facets exposure including cubes, octahedra and rhombic dodecahedra, as well as more complex polyhedra with high-index facets exposure such as 18-faceted, 26-faceted, 50-faceted and 74-faceted crystals. NaOH concentration is found to be the key factor in controlling Cu2O crystal morphology: as the concentration of NaOH increases, the morphology of Cu2O crystals gradually transforms from cubes that fully expose the {100} faces to regular polyhedra that expose the {110}, {111} faces, and even other high-index faces, ultimately presenting octahedra that fully expose the {111} faces. Additionally, Cu2O crystals with unique morphologies such as hollow cubes and 18-faceted with {110} face etched can be obtained by introducing surfactants or prolonging reaction durations. This work provides new insights into the morphology control of Cu2O crystals and establishes foundation in acquiring distinct Cu2O polyhedra in a facile manner for their application in catalysis, optoelectronics, sensing, and energy conversion fields.
Trend and Burden of Vitamin A Deficiency in 1990–2021 and Projection to 2050: A Systematic Analysis for the Global Burden of Disease Study 2021
Authors: Lin Kelly , Qi Yanfei , Sun Jing
Published: February 2025

Background/Objectives: In this study, we aim to provide an update on the global, regional, and national trends in VAD-associated mortality and morbidity for children under 20 years of age, across different age groups and sociodemographic backgrounds, to identify populations at risk that require further attention. Methods: Data from the Global Disease of Burden study were analysed to determine the temporal trends in VAD mortalities and VAD disease burden through disability-adjusted life years (DALYs) and Years Lived with Disability (YLD). Data on children under 20 years of age from 1990 to 2021 from 204 countries and territories were included for analysis. The Average Annual Percentage Change (AAPC) was used to show a temporal trend over a 30-year period. Results: Global VAD-associated mortality has decreased significantly, with an AAPC of −0.91 (95% CI= −0.95 to −0.85). No significant improvements in VAD morbidities were identified across Sub-Saharan African regions. In Central Sub-Saharan Africa, the number of VAD-associated disabilities increased from 70,032.12 to 73,534.15. Significant heterogeneity in changes in VAD morbidities were also identified across different countries. The highest age-standardized rate (ASR) of VAD YLD was 282.36 in Somalia, while countries with high sociodemographic indices had an ASR of 0. Conclusions: Significant global improvements in VAD mortalities indicate the efficacy of wide-scale high-dose vitamin A supplementation for children under 5 years of age. However, the lack of improvements in VAD morbidities in low-SDI countries highlights the need to continue crucial high-dose vitamin A supplementation and to implement additional vitamin A supplementation programs.
Self-Medication Practice and Associated Factors Among Health Professionals in Spain
Authors: Cotobal-Calvo Eva Manuela , Mata-Pérez Concepción , Bocchino Anna , Gilart Ester , Gutiérrez-Baena Belén , Palazón-Fernández José Luis
Published: February 2025

(1) Background: Self-medication, defined as the use of medications without professional supervision, is a common practice that presents both potential benefits and significant risks. This study analyzes the prevalence, patterns, and determinants of self-medication among health professionals in Spain. (2) Methods: A cross-sectional descriptive design was employed with 438 health professionals, predominantly women (81.1%), with a median age of 42 years. The majority of the healthcare workers were nurses (45%). (3) Results: The results revealed a high prevalence of self-medication (59.4%). Analgesics and anti-inflammatory drugs were the most commonly used. Age and professional knowledge emerged as significant factors influencing this behavior. The main reasons for self-medication included the mildness of symptoms, easy access to medications, and previous successful experiences. Digital sources, especially websites, were the most consulted. Confidence in artificial intelligence tools as a clinical resource was moderate, with 18% of participants consulting AI tools, a rate comparable to the 19.5% for scientific databases. Logistic regression analysis identified age, knowledge of recommended doses, and perceived efficacy as significant predictors, while concern about risks acted as a protective factor. (4) Conclusions: This study highlights the need for educational interventions aimed at promoting responsible self-medication practices and mitigating associated risks among healthcare professionals.
Detection of Leishmania donovani DNA from Oral Swab in Visceral Leishmaniasis
Authors: Sarkar Santana R. , Hobo Rina , Shoshi Yuki , Paul Shyamal K. , Goto Yasuyuki , Noiri Eisei , Matsumoto Yoshitsugu , Sanjoba Chizu
Published: February 2025

Visceral leishmaniasis (VL) is the most severe form of leishmaniasis and is fatal if left untreated in over 95% of cases. Leishmaniasis is one of the neglected tropical diseases that tend to thrive in developing regions of the world where inadequate access to healthcare makes it difficult for some people to even receive a diagnosis. This study examined the usefulness of oral swabs as specimens for VL diagnosis, by detecting Leishmania donovani DNA in oral swabs from both VL patients and L. donovani-infected mice. Eighty oral swab (OS) and blood buffy coat (BC) samples were collected from suspected VL cases in Bangladesh. These samples were evaluated using Leishmania kinetoplast minicircle DNA (kDNA) in real-time PCR, and the results showed that 62.5% (50/80) and 67.5% (54/80) of the cases tested positive for the BC specimen and OS, respectively. The OS positivity was statistically comparable to the BC. L. donovani DNA was also detected in an oral swab of all infected BALB/c mice by conventional PCR targeting the large subunit ribosomal RNA gene (LSUrRNA), while it was negative in uninfected mice. This study highlights the potential of practical methods for the molecular diagnosis of VL using oral swabs as a non-invasive, simple, and accurate approach.
Association of Altered Baseline Hematological Parameters with Adverse Tuberculosis Treatment Outcomes
Authors: Nancy Pandiarajan Arul , Kumar Nathella Pavan , Moideen Kadar , Thiruvengadam Kannan , Hissar Syed , Sivakumar Shanmugam , Bethunaickan Ramalingam , Viswanathan Vijay , Kornfeld Hardy , Babu Subash
Published: February 2025

Tuberculosis (TB) treatment monitoring is an essential tool for effective TB treatment management. Identifying parameters that predict adverse TB treatment outcomes could significantly improve clinical management. The association of hematological parameters with poor TB treatment outcomes is not well defined. To study the relationship of hematological parameters with TB treatment outcomes, we examined data from pulmonary tuberculosis (PTB) patients with successful (controls) and unsuccessful (cases) treatment outcomes. We enrolled 68 cases and 133 controls through a nested 1:2 case–control study, matching for age, sex, body mass index, diabetes status, alcohol and smoking. Hematological profiling showed significant difference in the absolute counts of white blood cells, lymphocytes, neutrophils and monocytes between cases and controls. In addition, increased neutrophil to lymphocyte ratio (NL) ratio and monocyte to lymphocyte (ML) ratio were present in cases in comparison to controls. Similarly, decreased hematocrit and red blood cell counts were detected in cases when compared with controls. Univariate and multivariate analysis demonstrated a significant association of absolute counts of WBC, neutrophils, monocytes, NL and ML ratios with poor treatment outcomes. The altered baseline hematological parameters are clearly associated with the poor TB treatment outcomes, showing potential for clinical prediction to enhance management of at-risk cases.
PEGylated Nanoliposomes Encapsulated with Anticancer Drugs for Breast and Prostate Cancer Therapy: An Update
Authors: Peter Sijongesonke , Khwaza Vuyolwethu , Alven Sibusiso , Naki Tobeka , Aderibigbe Blessing Atim
Published: February 2025

There are different types of cancer treatments, including surgery, radiotherapy, and chemotherapy. However, the complexity of cancer has resulted in treatment challenges to medicinal scientists and a socio-economic burden to the public health system globally. The pharmacological limitations associated with the current conventional anticancer drugs include lack of specificity, poor bioavailability, toxicity, drug resistance, and poor delivery mechanisms, which make cancer treatment challenging. Thus, the number of cancer cases is escalating rapidly, especially breast and prostate cancer in women and men, respectively. The application of nanoformulations is gaining momentum for treating different cancer types. However, they also exhibit challenges that must be addressed for effective cancer treatment. Nanoliposomes are nanoformulations that are widely explored for cancer treatment with interesting therapeutic outcomes. They have been functionalized with PEG to further improve their therapeutic outcomes. Hence, this review provides an update on PEGylated nanoliposomes loaded with anticancer drugs for the treatment of breast and prostate cancer, focusing on pre-clinical studies published in the last decade (2015 to 2024) to reflect the recent advancements made in the design of PEGylation nanoliposomes. Highlights of the clinically and commercially available PEGylation nanoliposomes are also presented in this review.
Exploratory Study on Nanoparticle Co-Delivery of Temozolomide and Ligustilide for Enhanced Brain Tumor Therapy
Authors: Ke Gang , Zhang Mingxia , Hu Pengyi , Zhang Jing , Naeem Abid , Wang Lianfang , Xu Huixin , Liu Yu , Cao Ming , Zheng Qin
Published: February 2025

Background: Temozolomide (TMZ) is the first-line therapy for glioblastoma (GBM), but its clinical efficacy is limited by its short half-life, poor brain targeting, adverse side effects, and the development of drug resistance. Ligustilide (LIG) has been shown to enhance blood-brain barrier permeability and reduce P-glycoprotein activity, thereby potentiating the synergistic effect of TMZ against GBM. Methods: The dual-drug-loaded nanoparticles encapsulating both TMZ and LIG (TMZ/LIG-NPs) were prepared using Poly (d,l-lactic-co-glycolide)-monomethoxy poly (ethylene glycol) (PLGA-mPEG). The physicochemical properties of the NPs, including particle size and zeta potential, were characterized. Cellular uptake of NPs was evaluated using flow cytometry and fluorescence staining. The pharmacokinetic profile and cytotoxicity of TMZ/LIG-NPs were compared to those of free TMZ and a mixture of TMZ and LIG in rat and glioma cells, respectively. Results: The mean particle size of TMZ/LIG-NPs was 117.6 ± 0.7 nm, with a zeta potential of −26.5 ± 0.4 mV. Cellular uptake of NPs was significantly higher than that of free drug in U251 cells. Encapsulation of TMZ in NPs significantly increased its half-life by 1.62-fold compared to free TMZ and significantly improved its pharmacokinetic profile. Moreover, the storage stability of the TMZ/LIG-NPs solution was extended to one month. The toxicity of TMZ/LIG-NPs to glioma cells C6 and U251 was markedly enhanced compared to the mixture of TMZ and LIG. Conclusions: The development of TMZ/LIG-NPs using PLGA-mPEG effectively enhanced the stability and efficacy of both TMZ and LIG. This dual drug-loaded nanoparticle system represents a promising strategy for glioblastoma therapy.