bluuebunny/crossref_metadata_2025 huggingface dataset:
Proposed Cool Coatings with High Near-Infrared Reflectance and Heat Insulation for Asphalt Pavement
Authors: Hu Kui , Chen Yujing , Chen Guixiang , Duan Yuzhou , Yu Caihua
Published: January 2021

In summer, black asphalt pavement can absorb a considerable amount of solar radiation, which causes its temperature to rise. Heated asphalt pavement can aggravate the urban heat island (UHI) effect and transfer heat downward, which may cause the problem of permafrost thawing beneath pavements. The objective of this study was to develop a novel cool coating layer (CCL) with high near-infrared reflectance and heat insulation to make the surface of asphalt pavement cool. A self-developed test device and method was established to evaluate cooling effects. Based on the experimental results, the optimal coating can cool asphalt pavement by 11.21 °C when the radiation striking the sample surface is 650 W/m2. This coating, called the composite cool coating layer (CCCL), is composed of the following materials: polyurethane resin, rutile TiO2 of 18%, hollow glass microspheres of 12%, and copper chromite black spinel of 0.7%. Silicon carbide particles of 1 kg/m2 can help the CCCL achieve satisfactory antiskid performance. In conclusion, CCCL can effectively inhibit the absorption of solar radiation and reduce the flow of thermal energy downward without sacrificing skid resistance.
Nuclear Imaging for Bone Metastases in Prostate Cancer: The Emergence of Modern Techniques Using Novel Radiotracers
Authors: Luining Wietske I. , Meijer Dennie , Dahele Max R. , Vis André N. , Oprea-Lager Daniela E.
Published: January 2021

Accurate staging of prostate cancer (PCa) at initial diagnosis and at biochemical recurrence is important to determine prognosis and the optimal treatment strategy. To date, treatment of metastatic PCa has mostly been based on the results of conventional imaging with abdominopelvic computed tomography (CT) and bone scintigraphy. However, these investigations have limited sensitivity and specificity which impairs their ability to accurately identify and quantify the true extent of active disease. Modern imaging modalities, such as those based on the detection of radioactively labeled tracers with combined positron emission tomography/computed tomography (PET/CT) scanning have been developed specifically for the detection of PCa. Novel radiotracers include 18F-sodium fluoride (NaF), 11C-/18F-fluorocholine (FCH), 18F-fluordihydrotestosterone (FDHT), 68Gallium and 18F-radiolabeled prostate-specific membrane antigen (e.g., 68Ga-PSMA-11, 18F-DCFPyL). PET/CT with these tracers outperforms conventional imaging. As a result of this, although their impact on outcome needs to be better defined in appropriate clinical trials, techniques like prostate-specific membrane antigen (PSMA) PET/CT have been rapidly adopted into clinical practice for (re)staging PCa. This review focuses on nuclear imaging for PCa bone metastases, summarizing the literature on conventional imaging (focusing on CT and bone scintigraphy—magnetic resonance imaging is not addressed in this review), highlighting the prognostic importance of high and low volume metastatic disease which serves as a driver for the development of better imaging techniques, and finally discussing modern nuclear imaging with novel radiotracers.
Surface Deformations Caused by the Convergence of Large Underground Gas Storage Facilities
Authors: Tajduś Krzysztof , Sroka Anton , Misa Rafał , Tajduś Antoni , Meyer Stefan
Published: January 2021

The article presents a method of forecasting the deformation of the land surface over large fields of underground gas storage facilities located in salt caverns. The solution allows for taking into account many parameters characterising the operation of underground gas storage facilities, such as cavern processes (leaching, enlargement, operational, etc.), their depth, distribution, diameter, shape, and many others. The advantage of the applied method over other available options is the possibility of using it for large fields of caverns while keeping the calculations simple. The effectiveness of the method has been proven for predicted surface subsidence for the EPE field with 114 underground caverns. The hypothesis was compared with the measurement outcomes.
Day-Ahead Electric Load Forecast for a Ghanaian Health Facility Using Different Algorithms
Authors: Chaaraoui Samer , Bebber Matthias , Meilinger Stefanie , Rummeny Silvan , Schneiders Thorsten , Sawadogo Windmanagda , Kunstmann Harald
Published: January 2021

Ghana suffers from frequent power outages, which can be compensated by off-grid energy solutions. Photovoltaic-hybrid systems become more and more important for rural electrification due to their potential to offer a clean and cost-effective energy supply. However, uncertainties related to the prediction of electrical loads and solar irradiance result in inefficient system control and can lead to an unstable electricity supply, which is vital for the high reliability required for applications within the health sector. Model predictive control (MPC) algorithms present a viable option to tackle those uncertainties compared to rule-based methods, but strongly rely on the quality of the forecasts. This study tests and evaluates (a) a seasonal autoregressive integrated moving average (SARIMA) algorithm, (b) an incremental linear regression (ILR) algorithm, (c) a long short-term memory (LSTM) model, and (d) a customized statistical approach for electrical load forecasting on real load data of a Ghanaian health facility, considering initially limited knowledge of load and pattern changes through the implementation of incremental learning. The correlation of the electrical load with exogenous variables was determined to map out possible enhancements within the algorithms. Results show that all algorithms show high accuracies with a median normalized root mean square error (nRMSE) <0.1 and differing robustness towards load-shifting events, gradients, and noise. While the SARIMA algorithm and the linear regression model show extreme error outliers of nRMSE >1, methods via the LSTM model and the customized statistical approaches perform better with a median nRMSE of 0.061 and stable error distribution with a maximum nRMSE of <0.255. The conclusion of this study is a favoring towards the LSTM model and the statistical approach, with regard to MPC applications within photovoltaic-hybrid system solutions in the Ghanaian health sector.
Adaptive Surrogate Estimation with Spatial Features Using a Deep Convolutional Autoencoder for CO2 Geological Sequestration
Authors: Jo Suryeom , Park Changhyup , Ryu Dong-Woo , Ahn Seongin
Published: January 2021

This paper develops a reliable deep-learning framework to extract latent features from spatial properties and investigates adaptive surrogate estimation to sequester CO2 into heterogeneous deep saline aquifers. Our deep-learning architecture includes a deep convolutional autoencoder (DCAE) and a fully-convolutional network to not only reduce computational costs but also to extract dimensionality-reduced features to conserve spatial characteristics. The workflow integrates two different spatial properties within a single convolutional system, and it also achieves accurate reconstruction performance. This approach significantly reduces the number of parameters to 4.3% of the original number required, e.g., the number of three-dimensional spatial properties needed decreases from 44,460 to 1920. The successful dimensionality reduction is accomplished by the DCAE system regarding all inputs as image channels from the initial stage of learning using the fully-convolutional network instead of fully-connected layers. The DCAE reconstructs spatial parameters such as permeability and porosity while conserving their statistical values, i.e., their mean and standard deviation, achieving R-squared values of over 0.972 with a mean absolute percentage error of their mean values of less than 1.79%. The adaptive surrogate model using the latent features extracted by DCAE, well operations, and modeling parameters is able to accurately estimate CO2 sequestration performances. The model shows R-squared values of over 0.892 for testing data not used in training and validation. The DCAE-based surrogate estimation exploits the reliable integration of various spatial data within the fully-convolutional network and allows us to evaluate flow behavior occurring in a subsurface domain.
Acknowledgment to Reviewers of Environments in 2020
Authors:   Environments Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Environments maintains its standards for the high quality of its published papers [...]
Association of Greenness with Blood Pressure among Individuals with Type 2 Diabetes across Rural to Urban Community Types in Pennsylvania, USA
Authors: Poulsen Melissa N. , Schwartz Brian S. , Nordberg Cara , DeWalle Joseph , Pollak Jonathan , Imperatore Giuseppina , Mercado Carla I. , Siegel Karen R. , Hirsch Annemarie G.
Published: January 2021

Greenness may impact blood pressure (BP), though evidence is limited among individuals with type 2 diabetes (T2D), for whom BP management is critical. We evaluated associations of residential greenness with BP among individuals with T2D in geographically diverse communities in Pennsylvania. To address variation in greenness type, we evaluated modification of associations by percent forest. We obtained systolic (SBP) and diastolic (DBP) BP measurements from medical records of 9593 individuals following diabetes diagnosis. Proximate greenness was estimated within 1250-m buffers surrounding individuals’ residences using the normalized difference vegetation index (NDVI) prior to blood pressure measurement. Percent forest was calculated using the U.S. National Land Cover Database. Linear mixed models with robust standard errors accounted for spatial clustering; models were stratified by community type (townships/boroughs/cities). In townships, the greenest communities, an interquartile range increase in NDVI was associated with reductions in SBP of 0.87 mmHg (95% CI: −1.43, −0.30) and in DBP of 0.41 mmHg (95% CI: −0.78, −0.05). No significant associations were observed in boroughs or cities. Evidence for modification by percent forest was weak. Findings suggest a threshold effect whereby high greenness may be necessary to influence BP in this population and support a slight beneficial impact of greenness on cardiovascular disease risk.
Urban Lighting Research Transdisciplinary Framework—A Collaborative Process with Lighting Professionals
Authors: Pérez Vega Catherine , Zielinska-Dabkowska Karolina M. , Hölker Franz
Published: January 2021

Over the past decades, lighting professionals have influenced the experience of the night by brightly illuminating streets, buildings, skylines, and landscapes 24/7. When this became the accepted norm, a dual perspective on night-time was shaped and the visual enjoyment of visitors after dusk was prioritized over natural nightscapes (nocturnal landscapes). During this time, researchers of artificial light at night (ALAN) observed and reported a gradual increase in unnatural brightness and a shift in color of the night-time environment. As a consequence, ALAN has been identified as a relevant pollutant of aquatic and terrestrial habitats, and an environmental stressor, which may adversely affect a wide range of organisms, from micro-organisms to humans. Unfortunately, lighting professionals and ALAN researchers usually attempt to solve today’s sustainable urban lighting problems distinctive to their fields of study, without a dialogue between research and practice. Therefore, in order to translate research knowledge as an applicable solution for the lighting practice and to minimize the impact on the environment, a collaborative framework involving a transdisciplinary process with lighting professionals is crucial to potentially bring the practice, research, production, decision-making, and planning closer to each other. This paper presents a framework to help reduce the existing gap of knowledge, because appropriate lighting applications depend upon it. Access to less light polluted nightscapes in urban environments is just as important as access to unpolluted water, food, and air. This call for action towards sustainable urban lighting should be included in future lighting policies to solve the urgent environmental and health challenges facing our world.
The Level of Lymphedema Awareness among Women with Breast Cancer in the Kingdom of Saudi Arabia
Authors: Alsharif Fatmah , Almutairi Wedad , Shibily Faygah , Alhothari Fatmah , Batwa Fidaa , Batwa Nidaa , Alharbi Lujain
Published: January 2021

Background: Lymphedema is a condition in which excessive fluid accumulates in soft tissues. It is a common complication of breast cancer treatments. It can lead to serious consequences and interfere with the activity of daily living. This study aimed to determine the level of awareness of breast-cancer-related lymphedema (BCRL) among women with breast cancer in the Kingdom of Saudi Arabia. This was a descriptive quantitative cross-sectional design that included a convenience sample of women diagnosed with breast cancer in the Kingdom of Saudi Arabia. Data were collected by distributing a self-administrated online questionnaire consisting of four parts, including demographic data (five items), the status of education about BCRL (three items), basic medical history of breast cancer (six items), and BCRL level of awareness of risk factors and management (nine items). Results: In total, 95 out of 135 of participants did not know about lymphedema, 119 of the participants (88.1%) did not receive any explanation about the possibility of lymphedema from their medical team before surgery, and 121 of them (89.6%) did not receive it after surgery. The most significant factor affecting participants’ level of awareness regarding BCRL was the lack of information about the possibility of BCRL occurrence, which was not provided to them by the medical team. Recommendation: Early and continuous education for future management is essential to prevent problems related to BCRL and improve quality of life.
Development of a Binational Framework for Active and Healthy Ageing (AHA) Bridging Austria and Slovenia in a Thermal Spa Region
Authors: Lindner Sonja , Illing Kai , Sommer Josef , Krajnc-Nikolić Tatjana , Harer Johann , Kurre Christoph , Lautner Karl , Hauser Mateja , Grabar Daniel , Graf-Stelzl Robert , Korn Christian , Pilz Klaus , Ritter Bernhard , Roller-Wirnsberger Regina
Published: January 2021

In view of ongoing demographic developments resulting in a longer life expectancy of the European population, the creation of “age-friendly” environments represents an initiative picked up by the European Union and its Member States to enable active and healthy ageing. The present study aims at the co-creation of a cross-border framework model to deploy a healthy ageing region linking Austria and Slovenia, building on previous work dealing with the development of an integrated regional ecosystem for active and healthy ageing. A qualitative, community-based action research method based on focus group discussions allowed the development of an exemplary framework model for active and healthy ageing building on cross-border collaboration in the region of Promura. Within the project group, twelve cross-border regional key assets were identified. In the course of further open discussions, an exemplary model for the deployment of a cross-border healthy ageing region was developed, comprising underlying fundamental environmental aspects, regional structures in the field of health and care as well as crosscutting features spreading across all levels. This article presents a promising, strategic co-creation approach on how to span a model on active and healthy ageing across two cross-border regions with similar characteristics and assets.
The Role of NF-κB in Uterine Spiral Arteries Remodeling, Insight into the Cornerstone of Preeclampsia
Authors: Socha Maciej W. , Malinowski Bartosz , Puk Oskar , Wartęga Mateusz , Stankiewicz Martyna , Kazdepka-Ziemińska Anita , Wiciński Michał
Published: January 2021

Preeclampsia is one of the three leading causes of maternal morbidity and mortality worldwide. It afflicts 2–8% of pregnancies and is the most common cause of gestational hypertension. This article is focused on nuclear factor kappa B (NF-κB), its role in normal and pathological spiral arteries remodelling and development of preeclampsia, with evaluation if it is a promising therapeutic target. NF-κB is a key mediator of placentation. Since insemination, it stimulates production of proinflammatory cytokines by the uterine epithelium, which leads to activation of macrophages, uterine natural killer cells (uNKs), and other leukocytes. The trophoblast/uNK/macrophage crosstalk is crucial for implantation and spiral arteries remodeling, and NF-κB regulates that process through modification of cytokine expression, as well as cell phenotype and function. In the course of preeclampsia, the remodeling processes is disturbed by excessive inflammation and increased NF-κB activation. The pathological remodeling leads to uteroplacental dysfunction, release of proinflammatory cytokines into the maternal circulation, endothelial stress, and development of preeclampsia. The analysis of genetic and environmental inductors of NF-κB helps to distinguish preeclampsia risk groups. Furthermore, a selective inhibition of NF-κB or NF-κB activating pathways alleviates symptoms of preeclampsia in rat models; therefore, this could be an efficient therapeutic option.
Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding
Authors: Cuy-Chaparro Laura , Bohórquez Michel David , Arévalo-Pinzón Gabriela , Castañeda-Ramírez Jeimmy Johana , Suárez Carlos Fernando , Pabón Laura , Ordóñez Diego , Gallego-López Gina Marcela , Suárez Carlos Esteban , Moreno-Pérez Darwin Andrés , Patarroyo Manuel Alfonso
Published: January 2021

Apical membrane antigen 1 is a microneme protein which plays an indispensable role during Apicomplexa parasite invasion. The detailed mechanism of AMA-1 molecular interaction with its receptor on bovine erythrocytes has not been completely defined in Babesia bovis. This study was focused on identifying the minimum B. bovis AMA-1-derived regions governing specific and high-affinity binding to its target cells. Different approaches were used for detecting ama-1 locus genetic variability and natural selection signatures. The binding properties of twelve highly conserved 20-residue-long peptides were evaluated using a sensitive and specific binding assay based on radio-iodination. B. bovis AMA-1 ectodomain structure was modelled and refined using molecular modelling software. NetMHCIIpan software was used for calculating B- and T-cell epitopes. The B. bovis ama-1 gene had regions under functional constraint, having the highest negative selective pressure intensity in the Domain I encoding region. Interestingly, B. bovis AMA-1-DI (100YMQKFDIPRNHGSGIYVDLG119 and 120GYESVGSKSYRMPVGKCPVV139) and DII (302CPMHPVRDAIFGKWSGGSCV321)-derived peptides had high specificity interaction with erythrocytes and bound to a chymotrypsin and neuraminidase-treatment sensitive receptor. DI-derived peptides appear to be exposed on the protein’s surface and contain predicted B- and T-cell epitopes. These findings provide data (for the first-time) concerning B. bovis AMA-1 functional subunits which are important for establishing receptor-ligand interactions which could be used in synthetic vaccine development.
Epigallocatechin Gallate Ameliorates the Effects of Prenatal Alcohol Exposure in a Fetal Alcohol Spectrum Disorder-Like Mouse Model
Authors: Almeida-Toledano Laura , Andreu-Fernández Vicente , Aras-López Rosa , García-Algar Óscar , Martínez Leopoldo , Gómez-Roig María Dolores
Published: January 2021

Fetal alcohol spectrum disorder is the main preventable cause of intellectual disability in the Western world. Although binge drinking is the most studied prenatal alcohol exposure pattern, other types of exposure, such as the Mediterranean, are common in specific geographic areas. In this study, we analyze the effects of prenatal alcohol exposure in binge and Mediterranean human drinking patterns on placenta and brain development in C57BL/6J mice. We also assess the impact of prenatal treatment with the epigallocatechin-3-gallate antioxidant in both groups. Study experimental groups for Mediterranean or binge patterns: (1) control; (2) ethanol; (3) ethanol + epigallocatechin-3-gallate. Brain and placental tissue were collected on gestational Day 19. The molecular pathways studied were fetal and placental growth, placental angiogenesis (VEGF-A, PLGF, VEGF-R), oxidative stress (Nrf2), and neurodevelopmental processes including maturation (NeuN, DCX), differentiation (GFAP) and neural plasticity (BDNF). Prenatal alcohol exposure resulted in fetal growth restriction and produced imbalances of placental angiogenic factors. Moreover, prenatal alcohol exposure increased oxidative stress and caused significant alterations in neuronal maturation and astrocyte differentiation. Epigallocatechin-3-gallate therapy ameliorated fetal growth restriction, attenuated alcohol-induced changes in placental angiogenic factors, and partially rescued neuronal nuclear antigen (NeuN), (doublecortin) DCX, and (glial fibrillary acidic protein) GFAP levels. Any alcohol consumption (Mediterranean or binge) during pregnancy may generate a fetal alcohol spectrum disorder phenotype and the consequences may be partially attenuated by a prenatal treatment with epigallocatechin-3-gallate.
Overcoming Obstacles to Targeting Muscarinic Receptor Signaling in Colorectal Cancer
Authors: Ali Osman , Tolaymat Mazen , Hu Shien , Xie Guofeng , Raufman Jean-Pierre
Published: January 2021

Despite great advances in our understanding of the pathobiology of colorectal cancer and the genetic and environmental factors that mitigate its onset and progression, a paucity of effective treatments persists. The five-year survival for advanced, stage IV disease remains substantially less than 20%. This review examines a relatively untapped reservoir of potential therapies to target muscarinic receptor expression, activation, and signaling in colorectal cancer. Most colorectal cancers overexpress M3 muscarinic receptors (M3R), and both in vitro and in vivo studies have shown that activating these receptors stimulates cellular programs that result in colon cancer growth, survival, and spread. In vivo studies using mouse models of intestinal neoplasia have shown that using either genetic or pharmacological approaches to block M3R expression and activation, respectively, attenuates the development and progression of colon cancer. Moreover, both in vitro and in vivo studies have shown that blocking the activity of matrix metalloproteinases (MMPs) that are induced selectively by M3R activation, i.e., MMP1 and MMP7, also impedes colon cancer growth and progression. Nonetheless, the widespread expression of muscarinic receptors and MMPs and their importance for many cellular functions raises important concerns about off-target effects and the safety of employing similar strategies in humans. As we highlight in this review, highly selective approaches can overcome these obstacles and permit clinicians to exploit the reliance of colon cancer cells on muscarinic receptors and their downstream signal transduction pathways for therapeutic purposes.
Myosin Binding Protein-C Forms Amyloid-Like Aggregates In Vitro
Authors: Bobyleva Liya G. , Shumeyko Sergey A. , Yakupova Elmira I. , Surin Alexey K. , Galzitskaya Oxana V. , Kihara Hiroshi , Timchenko Alexander A. , Timchenko Maria A. , Penkov Nikita V. , Nikulin Alexey D. , Suvorina Mariya Yu. , Molochkov Nikolay V. , Lobanov Mikhail Yu. , Fadeev Roman S. , Vikhlyantsev Ivan M. , Bobylev Alexander G.
Published: January 2021

This work investigated in vitro aggregation and amyloid properties of skeletal myosin binding protein-C (sMyBP-C) interacting in vivo with proteins of thick and thin filaments in the sarcomeric A-disc. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) found a rapid (5–10 min) formation of large (>2 μm) aggregates. sMyBP-C oligomers formed both at the initial 5–10 min and after 16 h of aggregation. Small angle X-ray scattering (SAXS) and DLS revealed sMyBP-C oligomers to consist of 7–10 monomers. TEM and atomic force microscopy (AFM) showed sMyBP-C to form amorphous aggregates (and, to a lesser degree, fibrillar structures) exhibiting no toxicity on cell culture. X-ray diffraction of sMyBP-C aggregates registered reflections attributed to a cross-β quaternary structure. Circular dichroism (CD) showed the formation of the amyloid-like structure to occur without changes in the sMyBP-C secondary structure. The obtained results indicating a high in vitro aggregability of sMyBP-C are, apparently, a consequence of structural features of the domain organization of proteins of this family. Formation of pathological amyloid or amyloid-like sMyBP-C aggregates in vivo is little probable due to amino-acid sequence low identity (<26%), alternating ordered/disordered regions in the protein molecule, and S–S bonds providing for general stability.
Mycobacteriophages as Potential Therapeutic Agents against Drug-Resistant Tuberculosis
Authors: Allué-Guardia Anna , Saranathan Rajagopalan , Chan John , Torrelles Jordi B.
Published: January 2021

The current emergence of multi-, extensively-, extremely-, and total-drug resistant strains of Mycobacterium tuberculosis poses a major health, social, and economic threat, and stresses the need to develop new therapeutic strategies. The notion of phage therapy against bacteria has been around for more than a century and, although its implementation was abandoned after the introduction of drugs, it is now making a comeback and gaining renewed interest in Western medicine as an alternative to treat drug-resistant pathogens. Mycobacteriophages are genetically diverse viruses that specifically infect mycobacterial hosts, including members of the M. tuberculosis complex. This review describes general features of mycobacteriophages and their mechanisms of killing M. tuberculosis, as well as their advantages and limitations as therapeutic and prophylactic agents against drug-resistant M. tuberculosis strains. This review also discusses the role of human lung micro-environments in shaping the availability of mycobacteriophage receptors on the M. tuberculosis cell envelope surface, the risk of potential development of bacterial resistance to mycobacteriophages, and the interactions with the mammalian host immune system. Finally, it summarizes the knowledge gaps and defines key questions to be addressed regarding the clinical application of phage therapy for the treatment of drug-resistant tuberculosis.
Mitophagy Modulation, a New Player in the Race against ALS
Authors: Madruga Enrique , Maestro Inés , Martínez Ana
Published: January 2021

Amyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease that usually results in respiratory paralysis in an interval of 2 to 4 years. ALS shows a multifactorial pathogenesis with an unknown etiology, and currently lacks an effective treatment. The vast majority of patients exhibit protein aggregation and a dysfunctional mitochondrial accumulation in their motoneurons. As a result, autophagy and mitophagy modulators may be interesting drug candidates that mitigate key pathological hallmarks of the disease. This work reviews the most relevant evidence that correlate mitophagy defects and ALS, and discusses the possibility of considering mitophagy as an interesting target in the search for an effective treatment for ALS.
Systemic Inflammation and the Breakdown of Intestinal Homeostasis Are Key Events in Chronic Spinal Cord Injury Patients
Authors: Diaz David , Lopez-Dolado Elisa , Haro Sergio , Monserrat Jorge , Martinez-Alonso Carlos , Balomeros Dimitrios , Albillos Agustín , Alvarez-Mon Melchor
Published: January 2021

Our aim was to investigate the subset distribution and function of circulating monocytes, proinflammatory cytokine levels, gut barrier damage, and bacterial translocation in chronic spinal cord injury (SCI) patients. Thus, 56 SCI patients and 28 healthy donors were studied. The levels of circulating CD14+highCD16−, CD14+highCD16+, and CD14+lowCD16+ monocytes, membrane TLR2, TLR4, and TLR9, phagocytic activity, ROS generation, and intracytoplasmic TNF-α, IL-1, IL-6, and IL-10 after lipopolysaccharide (LPS) stimulation were analyzed by polychromatic flow cytometry. Serum TNF-α, IL-1, IL-6 and IL-10 levels were measured by Luminex and LPS-binding protein (LBP), intestinal fatty acid-binding protein (I-FABP) and zonulin by ELISA. SCI patients had normal monocyte counts and subset distribution. CD14+highCD16− and CD14+highCD16+ monocytes exhibited decreased TLR4, normal TLR2 and increased TLR9 expression. CD14+highCD16− monocytes had increased LPS-induced TNF-α but normal IL-1, IL-6, and IL-10 production. Monocytes exhibited defective phagocytosis but normal ROS production. Patients had enhanced serum TNF-α and IL-6 levels, normal IL-1 and IL-10 levels, and increased circulating LBP, I-FABP, and zonulin levels. Chronic SCI patients displayed impaired circulating monocyte function. These patients exhibited a systemic proinflammatory state characterized by enhanced serum TNF-α and IL-6 levels. These patients also had increased bacterial translocation and gut barrier damage.
Cell-Based Tracers as Trojan Horses for Image-Guided Surgery
Authors: Sier Vincent Q. , de Vries Margreet R. , van der Vorst Joost R. , Vahrmeijer Alexander L. , van Kooten Cornelis , Cruz Luis J. , de Geus-Oei Lioe-Fee , Ferreira Valerie , Sier Cornelis F. M. , Alves Frauke , Muthana Munitta
Published: January 2021

Surgeons rely almost completely on their own vision and palpation to recognize affected tissues during surgery. Consequently, they are often unable to distinguish between different cells and tissue types. This makes accurate and complete resection cumbersome. Targeted image-guided surgery (IGS) provides a solution by enabling real-time tissue recognition. Most current targeting agents (tracers) consist of antibodies or peptides equipped with a radiolabel for Positron Emission Tomography (PET) and Single Photon Emission Computed Tomography (SPECT), magnetic resonance imaging (MRI) labels, or a near-infrared fluorescent (NIRF) dye. These tracers are preoperatively administered to patients, home in on targeted cells or tissues, and are visualized in the operating room via dedicated imaging systems. Instead of using these ‘passive’ tracers, there are other, more ‘active’ approaches of probe delivery conceivable by using living cells (macrophages/monocytes, neutrophils, T cells, mesenchymal stromal cells), cell(-derived) fragments (platelets, extracellular vesicles (exosomes)), and microorganisms (bacteria, viruses) or, alternatively, ‘humanized’ nanoparticles. Compared with current tracers, these active contrast agents might be more efficient for the specific targeting of tumors or other pathological tissues (e.g., atherosclerotic plaques). This review provides an overview of the arsenal of possibilities applicable for the concept of cell-based tracers for IGS.
Identification and Functional Analysis of Tomato TPR Gene Family
Authors: Zhou Xi’nan , Zheng Yangyang , Cai Zhibo , Wang Xingyuan , Liu Yang , Yu Anzhou , Chen Xiuling , Liu Jiayin , Zhang Yao , Wang Aoxue
Published: January 2021

Tomato (Solanum lycopersicum) as an important vegetable grown around the world is threatened by many diseases, which seriously affects its yield. Therefore, studying the interaction between tomato and pathogenic bacteria is biologically and economically important. The TPR (Tetratricopeptide repeat) gene family is a class of genes containing TPR conserved motifs, which are widely involved in cell cycle regulation, gene expression, protein degradation and other biological processes. The functions of TPR gene in Arabidopsis and wheat plants have been well studied, but the research on TPR genes in tomato is not well studied. In this study, 26 TPR gene families were identified using bioinformatics based on tomato genome data, and they were analyzed for subcellular localization, phylogenetic evolution, conserved motifs, tissue expression, and GO (Gene Ontology) analysis. The qRT-PCR was used to detect the expression levels of each member of the tomato TPR gene family (SlTPRs) under biological stress (Botrytis cinerea) and abiotic stress such as drought and abscisic acid (ABA). The results showed that members of the tomato TPR family responded to various abiotic stresses and Botrytis cinerea stress, and the SlTPR2 and SlTPR4 genes changed significantly under different stresses. Using VIGS (Virus-induced gene silencing) technology to silence these two genes, the silenced plants showed reduced disease resistance. It was also shown that TPR4 can interact with atpA which encodes a chloroplast ATP synthase CF1 α subunit. The above results provide a theoretical basis for further exploring the molecular mechanism of TPR-mediated resistance in disease defense, and also provide a foundation for tomato disease resistance breeding.
Advanced Complex Analysis of the Thermal Softening of Nitrided Layers in Tools during Hot Die Forging
Authors: Krawczyk Jakub , Widomski Paweł , Kaszuba Marcin
Published: January 2021

This article is devoted to the issues of thermal softening of materials in the surface layer of forging tools. The research covers numerical modeling of the forging process, laboratory tests of tempering of nitrided layers, and the analysis of tempering of the surface layer of tools in the actual forging process. Numerical modeling was supported by measuring the temperature inside the tools with a thermocouple inserted into the tool to measure the temperature as close to the surface as possible. The modeling results confirmed the possibility of tempering the die material. The results of laboratory tests made it possible to determine the influence of temperature on tempering at different surface layer depths. Numerical analysis and measurement of surface layer microhardness of tools revealed the destructive effect of temperature during forging on the tempering of the nitrided layer and on the material layers located deeper below the nitrided layer. The results have shown that in the hot forging processes carried out in accordance with the adopted technology, the surface layer of working tools is overheated locally to a temperature above 600 °C and tempering occurs. Moreover, overheating effects are visible, because the surface layer is tempered to a depth of 0.3 mm. Finally, such tempering processes lead to a decrease in the die hardness, which causes accelerated wear because of the abrasion and plastic deformation. The nitriding does not protect against the tempering phenomenon, but only delays the material softening process, because tempering occurs in the nitrided layer and in the layers deeper under the nitrided layer. Below the nitrided layer, tempering occurs relatively quickly and a soft layer is formed with a hardness below 400 HV.
Aluminothermic Reduction of Manganese Oxide from Selected MnO-Containing Slags
Authors: Kudyba Artur , Akhtar Shahid , Johansen Inge , Safarian Jafar
Published: January 2021

The aluminothermic reduction process of manganese oxide from different slags by aluminum was investigated using pure Al and two types of industrial Al dross. Two types of MnO-containing slags were used: a synthetic highly pure CaO-MnO slag and an industrial high carbon ferromanganese slag. Mixtures of Al and slag with more Al than the stoichiometry were heated and interacted in an induction furnace up to 1873 K, yielding molten metal and slag products. The characterization of the produced metal and slag phases indicated that the complete reduction of MnO occurs via the aluminothermic process. Moreover, as the Al content in the charge was high, it also completely reduced SiO2 in the industrial ferromanganese slag. A small mass transport of Ca and Mg into the metal phase was also observed, which was shown to be affected by the slag chemistry. The obtained results indicated that the valorization of both Al dross and FeMn slag in a single process for the production of Mn, Mn-Al, and Mn-Al-Si alloys is possible. Moreover, the energy balance for the process indicated that the energy consumption of the process to produce Mn-Al alloys via the proposed process is insignificant due to the highly exothermic reactions at high temperatures.
Thermoplastic Polymers with Nanosilver Addition—Microstructural, Surface and Mechanical Evaluation during a 36-Month Deionized Water Incubation Period
Authors: Ziąbka Magdalena , Dziadek Michał
Published: January 2021

Three types of thermoplastic polymers, acrylonitrile butadiene styrene (ABS), polymethyl methacrylate acrylic (PMMA) and high-density polyethylene (HDPE), were enriched with silver nanoparticles (AgNPs) of 0.5 wt.% and 1.0 wt.%, respectively. The polymers and the composites were manufactured via injection molding. Regarding the potential of these polymers as matrices for long-term use as biomaterials, the aim of this study was to examine their stability in the in vitro conditions during a three-year incubation period in deionized water. In this work, microstructural observations were performed, and mechanical properties were assessed. Surface parameters, such as roughness and contact angle, were comprehensively investigated. The microstructural evaluation showed that the silver additive was homogeneously dispersed in all the examined matrices. The 36-month immersion period indicated no microstructural changes and proved the composites’ stability. The mechanical tests confirmed that the composites retained comparable mechanical properties after the silver incorporation. The Young’s modulus and tensile strength increased during long-term incubation. The addition of silver nanoparticles did not alter the composites’ roughness. The contact angle increased with the rising AgNP content. It was also shown that the materials’ roughness increased with the incubation time, especially for the ABS- and HDPE-based materials. The water environment conditions improved the wettability of the tested materials. However, the silver nanoparticles’ content resulted in the contact angle decreasing during incubation. The conducted studies confirmed that the mechanical properties of all the polymers and composites did not deteriorate; thus, the materials may be considered stable and applicable for long-term working periods in aqueous environments.
Sulfonyl Imide Acid-Functionalized Membranes via Ni (0) Catalyzed Carbon-Carbon Coupling Polymerization for Fuel Cells
Authors: Sutradhar Sabuj Chandra , Yoon Sujin , Ryu Taewook , Jin Lei , Zhang Wei , Jang Hohyoun , Kim Whangi
Published: January 2021

Polymer membranes, having improved conductivity with enhanced thermal and chemical stability, are desirable for proton exchange membranes fuel cell application. Hence, poly(benzophenone)s membranes (SI-PBP) containing super gas-phase acidic sulfonyl imide groups have been prepared from 2,5-dichlorobenzophenone (DCBP) monomer by C-C coupling polymerization using Ni (0) catalyst. The entirely aromatic C-C coupled polymer backbones of the SI-PBP membranes provide exceptional dimensional stability with rational ion exchange capacity (IEC) from 1.85 to 2.30 mS/cm. The as-synthesized SI-PBP membranes provide enhanced proton conductivity (107.07 mS/cm) compared to Nafion 211® (104.5 mS/cm). The notable thermal and chemical stability of the SI-PBP membranes have been assessed by the thermogravimetric analysis (TGA) and Fenton’s test, respectively. The well distinct surface morphology of the SI-PBP membranes has been confirmed by the atomic force microscopy (AFM). These results of SI-PBP membranes comply with all the requirements for fuel cell applications.
Chemical Degradation of PSF-PUR Blend Hollow Fiber Membranes—Assessment of Changes in Properties and Morphology after Hydrolysis
Authors: Sikorska Wioleta , Wasyłeczko Monika , Przytulska Małgorzata , Wojciechowski Cezary , Rokicki Gabriel , Chwojnowski Andrzej
Published: January 2021

In this study, we focused on obtaining polysulfone-polyurethane (PSF-PUR) blend partly degradable hollow fiber membranes (HFMs) with different compositions while maintaining a constant PSF:PUR = 8:2 weight ratio. It was carried out through hydrolysis, and evaluation of the properties and morphology before and after the hydrolysis process while maintaining a constant cut-off. The obtained membranes were examined for changes in ultrafiltration coefficient (UFC), retention, weight loss, morphology assessment using scanning electron microscopy (SEM) and MeMoExplorer™ Software, as well as using the Fourier-transform infrared spectroscopy (FT-IR) method. The results of the study showed an increase in the UFC value after the hydrolysis process, changes in retention, mass loss, and FT-IR spectra. The evaluation in MeMoExplorer™ Software showed the changes in membranes’ morphology. It was confirmed that polyurethane (PUR) was partially degraded, the percentage of ester bonds has an influence on the degradation process, and PUR can be used as a pore precursor instead of superbly known polymers.
Process Window for Electron Beam Melting of 316LN Stainless Steel
Authors: Roos Stefan , Rännar Lars-Erik
Published: January 2021

Electron beam melting (EBM) is currently hampered by the low number of materials available for processing. This work presents an experimental study of process parameter development related to EBM processing of stainless steel alloy 316LN. Area energy (AE) input and beam deflection rate were varied to produce a wide array of samples in order to determine which combination of process parameters produced dense (>99%) material. Both microstructure and tensile properties were studied. The aim was to determine a process window which results in dense material. The range of AE which produced dense materials was found to be wider for 316LN than for many other reported materials, especially at lower beam deflection rates. Tensile and microstructural analysis showed that increasing the beam deflection rate, and consequently lowering the AE, resulted in material with a smaller grain size, lower ductility, lower yield strength, and a narrower window for producing material that is neither porous nor swelling.
Azulene—A Bright Core for Sensing and Imaging
Authors: Murfin Lloyd C. , Lewis Simon E.
Published: January 2021

Azulene is a hydrocarbon isomer of naphthalene known for its unusual colour and fluorescence properties. Through the harnessing of these properties, the literature has been enriched with a series of chemical sensors and dosimeters with distinct colorimetric and fluorescence responses. This review focuses specifically on the latter of these phenomena. The review is subdivided into two sections. Section one discusses turn-on fluorescent sensors employing azulene, for which the literature is dominated by examples of the unusual phenomenon of azulene protonation-dependent fluorescence. Section two focuses on fluorescent azulenes that have been used in the context of biological sensing and imaging. To aid the reader, the azulene skeleton is highlighted in blue in each compound.
Inhibitory Effect of Thai Purple Rice Husk Extract on Chemically Induced Carcinogenesis in Rats
Authors: Punvittayagul Charatda , Chariyakornkul Arpamas , Sankam Paweena , Wongpoomchai Rawiwan
Published: January 2021

This study investigated the cancer chemopreventive effects of an acidic methanol extract of purple rice husk on chemically induced carcinogenesis in rats. This purple rice husk extract (PRHE) had high polyphenol contents. Vanillic acid was a major phenolic compound in PRHE. Three major anthocyanins found in PRHE were malvidin-3-glucoside, peonidin-3-glucoside and cyanidin-3-glucoside. PRHE was not toxic and clastogenic in rats. The LD50 of PRHE was greater than 2000 mg kg−1 body weight (BW). The oral administration of 300 or 1000 mg kg−1 BW of PRHE for 28 days significantly decreased the number of micronucleated hepatocytes in diethylnitrosamine-initiated rats. The inhibitory mechanisms were associated with the reduction of cytochrome P450 2E1 expression and induction of some detoxifying enzymes in the liver. In addition, treatment with 500 mg kg−1 BW of PRHE for eight weeks did not induce preneoplastic lesions in the liver and colon. It significantly inhibited hepatic glutathione-S-transferase positive foci formation induced by diethylnitrosamine and 1,2-dimethylhydrazine by suppression of hepatocyte proliferation and induction of apoptosis. In conclusion, PRHE did not present toxicity, clastogenicity or carcinogenicity in rats. It exhibited cancer chemopreventive properties against chemically induced early stages rat hepatocarcinogenesis. Anthocyanins and vanillic acid might be candidate anticarcinogenic compounds in purple rice husk.
The Chemical Constituents from Fruits of Catalpa bignonioides Walt. and Their α-Glucosidase Inhibitory Activity and Insulin Secretion Effect
Authors: Oh Youngse , Lee Dahae , Park SeonJu , Kim Seung Hyun , Kang Ki Sung
Published: January 2021

Catalpa pod has been used in traditional medicine for the treatment of diabetes mellitus in South America. Studies on the constituents of Catalpa species have shown that it is rich in iridoids. In the present study, three previously undescribed compounds (2–4), including two secoiridoid derivatives along with twelve known compounds, were isolated from the fruits of Catalpa bignonioides Walt. In addition, fully assigned 13C-NMR of 5,6-dihydroxy-7,4’-dimethoxyflavone-6-O-sophoroside (1) is reported for the first time in the present study. The structures of compounds were determined on the basis of extensive spectroscopic methods, including UV, IR, 1D, and 2D NMR, mass spectroscopy, and CD spectroscopic data. All the isolated compounds were evaluated for α-glucosidase inhibitory activity. Among the tested compounds, compounds 2, 3, and 9 exhibited significant inhibitory activity against α-glucosidase enzyme assay. Meanwhile, the effect of compounds 2, 3, and 9 on glucose-stimulated insulin secretion (GSIS) was measured using pancreatic β-cells. Compounds 2, 3, and 9 exhibited non-cytotoxicity-stimulated insulin secretion in INS-1 cells. The expression levels of proteins associated with β-cell function and insulin secretion such as phosphorylation of total insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), Akt, activated pancreatic duodenal homeobox-1 (PDX-1), and peroxisome proliferator-activated receptor-γ (PPAR-γ) were increased in INS-1 cells after treatment with compounds 2, 3, and 9. The findings of the present study could provide a scientific warrant for their application as a potential antidiabetic agent.