bluuebunny/crossref_metadata_2025 huggingface dataset:
Computing Open Locating-Dominating Number of Some Rotationally-Symmetric Graphs
Authors: Raza Hassan
Published: June 2021

Location detection is studied for many scenarios, such as pointing out the flaws in multiprocessors, invaders in buildings and facilities, and utilizing wireless sensor networks for monitoring environmental processes. The system or structure can be illustrated as a graph in each of these applications. Sensors strategically placed at a subset of vertices can determine and identify irregularities within the network. The open locating-dominating set S of a graph G=(V,E) is the set of vertices that dominates G, and for any i,j∈ V(G) N(i)∩S≠N(j)∩S is satisfied. The set S is called the OLD-set of G. The cardinality of the set S is called open locating-dominating number and denoted by γold(G). In this paper, we computed exact values of the prism and prism-related graphs, and also the exact values of convex polytopes of Rn and Hn. The upper bound is determined for other classes of convex polytopes. The graphs considered here are well-known from the literature.
Association of Urinary Dickkopf-3 with Residual Renal Function Decline in Patients Undergoing Peritoneal Dialysis
Authors: Torigoe Kenta , Muta Kumiko , Tsuji Kiyokazu , Yamashita Ayuko , Torigoe Miki , Abe Shinichi , Ota Yuki , Mukae Hiroshi , Nishino Tomoya
Published: June 2021

Background and Objectives: Urinary levels of dickkopf-3 (DKK-3) are associated with poor renal survival in patients with non-dialytic chronic kidney disease. However, it remains unknown whether urinary DKK-3 levels can predict residual renal function (RRF) decline in patients undergoing peritoneal dialysis (PD). Therefore, we investigated the correlation between urinary levels of DKK-3 and the subsequent rate of RRF decline in PD patients. Materials and Methods: This study included 36 PD patients who underwent multiple peritoneal equivalent tests during 2011–2021. The relationship between baseline clinical characteristics and the subsequent annual rate of Kt/V decline was investigated. Results: The annual rate of renal Kt/V decline was 0.29 (range: 0.05–0.48), which correlated with renal Kt/V (r = 0.55, p = 0.0005) and 24 h urinary DKK-3 excretion (r = 0.61, p < 0.0001). Similarly, 24 h urinary DKK-3 excretion (β = 0.44, p = 0.0015) and renal Kt/V (β = 0.38, p = 0.0059) were independently associated with the annual rate of renal Kt/V decline in multivariate analyses. Conclusions: Urinary DKK-3 assessment may help identify PD patients at a high risk of RRF decline.
The Riga East University Hospital Stroke Registry—An Analysis of 4915 Consecutive Patients with Acute Stroke
Authors: Karelis Guntis , Micule Madara , Klavina Evija , Haritoncenko Iveta , Kikule Ilga , Tilgale Biruta , Polaka Inese
Published: June 2021

Background and Objectives: A hospital-based stroke registry is a useful tool for systematic analyses of the epidemiology, clinical characteristics, and natural course of stroke. Analyses of stroke registry data can provide information that can be used by health services to improve the quality of care for patients with this disease. Materials and Methods: Data were collected from the Riga East University Hospital (REUH) Stroke Registry in order to evaluate the etiology, risk factors, clinical manifestations, treatment, functional outcomes, and other relevant data for acute stroke during the period 2016–2020. Results: During a five-year period, 4915 patients (3039 females and 1876 males) with acute stroke were registered in the REUH Stroke Registry. The causative factors of stroke were cardioembolism (45.7%), atherosclerosis (29.9%), lacunar stroke (5.3%), stroke of undetermined etiology (1.2%), and stroke of other determined causes (1.2%). The most frequent localizations of intracerebral hemorrhage were subcortical (40.0%), lobar (18.9%), and brainstem (9.3%). The most prevalent risk factors for stroke were hypertension (88.8%), congestive heart failure (71.2%), dyslipidemia (46.7%), and atrial fibrillation (44.2%). In addition, 1018 (20.7%) patients were receiving antiplatelet drugs, 574 (11.7%) were taking statins, and 382 (7.7%) were taking anticoagulants. At discharge, 35.5% of the patients were completely independent (mRS (modified Rankin Scale) score: 0–2), while 49.5% required some form of assistance (mRS score: 3–5). The intrahospital mortality rate was 13.7%, although it was higher in the hemorrhage group (30.9%). Conclusions: Our stroke registry data are comparable to those of other major registries. Analysis of stroke registry data is important for improving stroke care and obtaining additional information for stroke studies.
Lithium Medication in Pregnancy and Breastfeeding—A Case Series
Authors: Gehrmann Andrea , Fiedler Katrin , Leutritz Anna Linda , Koreny Carolin , Kittel-Schneider Sarah
Published: June 2021

Lithium salts are the first-line prophylaxis treatment for bipolar disorder in most guidelines. The majority of bipolar women are treated with mood stabilizers at the time they wish to get pregnant. One reason for this is the rising average age at first childbirth, at least in the high-income countries, which increases in general the likelihood of a medication with psychotropic drugs. Previously, lithium exposition during pregnancy was thought to strongly increase the risk of severe cardiac malformation. However, recent studies only point to a low teratogenic risk, so nowadays an increasing number of women are getting pregnant with ongoing lithium treatment. Regarding lithium medication during breastfeeding, there is evidence that lithium transfers to the breastmilk and can also be detected in the infants’ serum. The influence on the infant is still a largely understudied topic. Regular monitoring of the infants’ renal clearance, thyroid function, and lithium levels is warranted when breastfeeding under lithium exposure. In this case series, we present three case reports of bipolar mothers who were treated with lithium during pregnancy and breastfeeding to add to the scarce literature on this important topic. In short, we strengthen the importance of therapeutic drug monitoring due to fluctuating plasma levels during pregnancy and after birth, and we can report the birth and development of three healthy infants despite lithium medication during pregnancy and breastfeeding.
The Effects of Fasting or Ketogenic Diet on Endurance Exercise Performance and Metabolism in Female Mice
Authors: Holcomb Lola E. , O’Neill Caitlin C. , DeWitt Elizabeth A. , Kolwicz Stephen C.
Published: June 2021

The promotion of ketone body (KB) metabolism via ketosis has been suggested as a strategy to increase exercise performance. However, studies in humans and animals have yielded inconsistent results. The purpose of the current study was to examine the effects of ketosis, achieved via fasting or a short-term ketogenic diet (KD), on endurance exercise performance in female mice. After 8 h of fasting, serum KB significantly increased and serum glucose significantly decreased in fasted compared to fed mice. When subjected to an endurance exercise capacity (EEC) test on a motorized treadmill, both fed and fasted mice showed similar EEC performance. A 5-week KD (90% calories from fat) significantly increased serum KB but did not increase EEC times compared to chow-fed mice. KD mice gained significantly more weight than chow-fed mice and had greater adipose tissue mass. Biochemical tissue analysis showed that KD led to significant increases in triglyceride content in the heart and liver and significant decreases in glycogen content in the muscle and liver. Furthermore, KD downregulated genes involved in glucose and KB oxidation and upregulated genes involved in lipid metabolism in the heart. These findings suggest that a short-term KD is not an effective strategy to enhance exercise performance and may lead to increased adiposity, abnormal endogenous tissue storage, and cardiometabolic remodeling.
A Metzincin and TIMP-Like Protein Pair of a Phage Origin Sensitize Listeria monocytogenes to Phage Lysins and Other Cell Wall Targeting Agents
Authors: Boichis Etai , Sigal Nadejda , Borovok Ilya , Herskovits Anat A.
Published: June 2021

Infection of mammalian cells by Listeria monocytogenes (Lm) was shown to be facilitated by its phage elements. In a search for additional phage remnants that play a role in Lm’s lifecycle, we identified a conserved locus containing two XRE regulators and a pair of genes encoding a secreted metzincin protease and a lipoprotein structurally similar to a TIMP-family metzincin inhibitor. We found that the XRE regulators act as a classic CI/Cro regulatory switch that regulates the expression of the metzincin and TIMP-like genes under intracellular growth conditions. We established that when these genes are expressed, their products alter Lm morphology and increase its sensitivity to phage mediated lysis, thereby enhancing virion release. Expression of these proteins also sensitized the bacteria to cell wall targeting compounds, implying that they modulate the cell wall structure. Our data indicate that these effects are mediated by the cleavage of the TIMP-like protein by the metzincin, and its subsequent release to the extracellular milieu. While the importance of this locus to Lm pathogenicity remains unclear, the observation that this phage-associated protein pair act upon the bacterial cell wall may hold promise in the field of antibiotic potentiation to combat antibiotic resistant bacterial pathogens.
Transposon Mutagenesis of Pseudomonas syringae Pathovars syringae and morsprunorum to Identify Genes Involved in Bacterial Canker Disease of Cherry
Authors: Neale Helen C. , Hulin Michelle T. , Harrison Richard J. , Jackson Robert W. , Arnold Dawn L.
Published: June 2021

Bacterial canker of Prunus, affecting economically important stone fruit crops including cherry, peach, apricot and plum, is caused by the plant pathogen Pseudomonas syringae (P.s.). Strains from two pathovars—P.s. pv. syringae (Pss) and P.s. pv. morsprunorum race 1 (PsmR1) and 2 (PsmR2)—in three phylogenetically distant clades have convergently evolved to infect Prunus. The bacteria enter woody tissues through wounds and leaf scars, causing black necrotic cankers. Symptoms are also produced on blossom, fruit and leaves. Little is known about the mechanisms P.s. uses to colonise tree hosts such as Prunus. Here, we created transposon (Tn) mutant libraries in one strain of P.s. from each of the three clades and screened the mutants on immature cherry fruit to look for changes in virulence. Mutants (242) with either reduced or enhanced virulence were detected and further characterised by in vitro screens for biofilm formation, swarming ability, and pathogenicity on leaves and cut shoots. In total, 18 genes affecting virulence were selected, and these were involved in diverse functions including motility, type III secretion, membrane transport, amino acid synthesis, DNA repair and primary metabolism. Interestingly, mutation of the effector gene, hopAU1, led to an increase in virulence of Psm R2.
Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities
Authors: Lopes Lucas Dantas , Chai Yen Ning , Marsh Ellen L. , Rajewski John F. , Dweikat Ismail , Schachtman Daniel P.
Published: June 2021

The belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial communities of seven sweet sorghum genotypes differing in sensitivity to N-stress. Sorghum genotypes were grown in fields with no (low-N) or sufficient (high-N) N. The dry shoot weight ratio (low-N/high-N) was used to determine N-stress sensitivity. Our hypothesis was that genotypes tolerant and sensitive to N-stress select distinct bacterial communities. The endosphere and rhizosphere bacterial community structure were significantly different between the N-stress sensitive and tolerant genotypes in the high-N field, but not in the low-N field. However, significant changes in the relative abundance of specific bacterial taxa were observed in both fields. Streptomyces, a bacterial genus known to alleviate plant abiotic stresses, was enriched in the endosphere and rhizosphere of the tolerant genotypes in the low-N field. Our study indicates that sweet sorghum genotypes tolerant to N-stress select taxa that can potentially mitigate the N-stress, suggesting that the interactions between N-stress tolerant lines and the root-associated microbiome might be vital for coping with N-stress.
Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water
Authors: Jéglot Arnaud , Sørensen Sebastian Reinhold , Schnorr Kirk M. , Plauborg Finn , Elsgaard Lars
Published: June 2021

Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3−) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3− removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3− depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes’ composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3− removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.
Characterization of βN-Octadecanoyl-5-hydroxytryptamide Anti-Inflammatory Effect
Authors: Giorno Thais Biondino Sardella , Lima Fernanda Alves , Brand Ana Laura Macedo , Oliveira Camila Martins de , Rezende Claudia Moraes , Fernandes Patricia Dias
Published: June 2021

Background: N-octadecanoyl-5-hydroxytryptamide (C18-5HT) is an amide that can be obtained by the coupling of serotonin and octadecanoic acid. This study aims to characterize the in vivo and in vitro anti-inflammatory activity of C18-5HT. Methods: A subcutaneous air pouch model (SAP) was used. The exudates were collected from SAP after carrageenan injection to assess cell migration and inflammatory mediators production. RAW 264.7 cells were used for in vitro assays. Results: C18-5HT significantly inhibited leukocyte migration into the SAP as well as nitric oxide (NO) and cytokines production and protein extravasation. We also observed an reduction in some cytokines and an increase in IL-10 production. Assays conducted with RAW 264.7 cells indicated that C18-5HT inhibited NO and cytokine produced. Conclusions: Taken together, our data suggest that C18-5HT presents a significant effect in different cell types (leukocytes collected from exudate, mainly polumorphonuclear leukocytes and cell culture macrophages) and is a promising compound for further studies for the development of a new anti-inflammatory drug.
Special Issue Editorial: Current Advances in Liquid Crystals
Authors: Bhowmik Pradip K.
Published: June 2021

The broad field of liquid crystals (LCs) has attracted the attention of chemists, physicists, biologists and engineers alike since the discovery of liquid crystalline phase by the Austrian botanist Friedrich Reinitzer in 1888 [...]
Benzylated Dihydroflavones and Isoquinoline-Derived Alkaloids from the Bark of Diclinanona calycina (Annonaceae) and Their Cytotoxicities
Authors: Costa Emmanoel V. , Soares Liviane do N. , Chaar Jamal da Silva , Silva Valdenizia R. , Santos Luciano de S. , Koolen Hector H. F. , Silva Felipe M. A. da , Tavares Josean F. , Zengin Gokhan , Soares Milena B. P. , Bezerra Daniel P.
Published: June 2021

Diclinanona calycina R. E. Fries popularly known as “envira”, is a species of the Annonaceae family endemic to Brazil. In our ongoing search for bioactive compounds from Annonaceae Amazon plants, the bark of D. calycina was investigated by classical chromatography techniques that yielded thirteen compounds (alkaloids and flavonoids) described for the first time in D. calycina as well as in the genus Diclinanona. The structure of these isolated compounds were established by extensive analysis using 1D/2D-NMR spectroscopy in combination with MS. The isolated alkaloids were identified as belonging to the subclasses: simple isoquinoline, thalifoline (1); aporphine, anonaine (2); oxoaporphine, liriodenine (3); benzyltetrahydroisoquinolines, (S)-(+)-reticuline (4); dehydro-oxonorreticuline (3,4-dihydro-7-hydroxy-6-methoxy-1-isoquinolinyl)(3-hydroxy-4-methoxyphenyl)-methanone) (5); (+)-1S,2R-reticuline Nβ-oxide (6); and (+)-1S,2S-reticuline Nα-oxide (7); tetrahydroprotoberberine, coreximine (8); and pavine, bisnorargemonine (9). While the flavonoids belong to the benzylated dihydroflavones, isochamanetin (10), dichamanetin (11), and a mixture of uvarinol (12) and isouvarinol (13). Compound 5 is described for the first time in the literature as a natural product. The cytotoxic activity of the main isolated compounds was evaluated against cancer and non-cancerous cell lines. Among the tested compounds, the most promising results were found for the benzylated dihydroflavones dichamanetin (10), and the mixture of uvarinol (12) and isouvarinol (13), which presented moderate cytotoxic activity against the tested cancer cell lines (<20.0 µg·mL−1) and low cytotoxicity against the non-cancerous cell line MRC-5 (>25.0 µg·mL−1). Dichamanetin (11) showed cytotoxic activity against HL-60 and HCT116 with IC50 values of 15.78 µg·mL−1 (33.70 µmol·L−1) and 18.99 µg·mL−1 (40.56 µmol·L−1), respectively while the mixture of uvarinol (12) and isouvarinol (13) demonstrated cytotoxic activity against HL-60, with an IC50 value of 9.74 µg·mL−1, and HCT116, with an IC50 value of 17.31 µg·mL−1. These cytotoxic activities can be attributed to the presence of one or more hydroxybenzyl groups present in these molecules as well as the position in which these groups are linked. The cytotoxic activities of reticuline, anonaine and liriodenine have been previously established, with liriodenine being the most potent compound.
Micro-Raman Characterization of Structural Features of High-k Stack Layer of SOI Nanowire Chip, Designed to Detect Circular RNA Associated with the Development of Glioma
Authors: Ivanov Yuri D. , Malsagova Kristina A. , Popov Vladimir P. , Kupriyanov Igor N. , Pleshakova Tatyana O. , Galiullin Rafael A. , Ziborov Vadim S. , Dolgoborodov Alexander Yu. , Petrov Oleg F. , Miakonkikh Andrey V. , Rudenko Konstantin V. , Glukhov Alexander V. , Smirnov Alexander Yu. , Usachev Dmitry Yu. , Gadzhieva Olga A. , Bashiryan Boris A. , Shimansky Vadim N. , Enikeev Dmitry V. , Potoldykova Natalia V. , Archakov Alexander I.
Published: June 2021

The application of micro-Raman spectroscopy was used for characterization of structural features of the high-k stack (h-k) layer of “silicon-on-insulator” (SOI) nanowire (NW) chip (h-k-SOI-NW chip), including Al2O3 and HfO2 in various combinations after heat treatment from 425 to 1000 °C. After that, the NW structures h-k-SOI-NW chip was created using gas plasma etching optical lithography. The stability of the signals from the monocrine phase of HfO2 was shown. Significant differences were found in the elastic stresses of the silicon layers for very thick (>200 nm) Al2O3 layers. In the UV spectra of SOI layers of a silicon substrate with HfO2, shoulders in the Raman spectrum were observed at 480–490 cm−1 of single-phonon scattering. The h-k-SOI-NW chip created in this way has been used for the detection of DNA-oligonucleotide sequences (oDNA), that became a synthetic analog of circular RNA–circ-SHKBP1 associated with the development of glioma at a concentration of 1.1 × 10−16 M. The possibility of using such h-k-SOI NW chips for the detection of circ-SHKBP1 in blood plasma of patients diagnosed with neoplasm of uncertain nature of the brain and central nervous system was shown.
NbCl5-Mg Reagent System in Regio- and Stereoselective Synthesis of (2Z)-Alkenylamines and (3Z)-Alkenylols from Substituted 2-Alkynylamines and 3-Alkynylols
Authors: Kadikova Rita N. , Gabdullin Azat M. , Mozgovoj Oleg S. , Ramazanov Ilfir R. , Dzhemilev Usein M.
Published: June 2021

The reduction of N,N-disubstituted 2-alkynylamines and substituted 3-alkynylols using the NbCl5–Mg reagent system affords the corresponding dideuterated (2Z)-alkenylamine and (3Z)-alkenylol derivatives in high yields in a regio- and stereoselective manner through the deuterolysis (or hydrolysis). The reaction of substituted propargylamines and homopropargylic alcohols with the in situ generated low-valent niobium complex (based on the reaction of NbCl5 with magnesium metal) is an efficient tool for the synthesis of allylamines and homoallylic alcohols bearing a 1,2-disubstituted double bond. It was found that the well-known approach for the reduction of alkynes based on the use of the TaCl5-Mg reagent system does not work for 2-alkynylamines and 3-alkynylols. Thus, this article reveals a difference in the behavior of two reagent systems—NbCl5-Mg and TaCl5-Mg, in relation to oxygen- and nitrogen-containing alkynes. A regio- and stereoselective method was developed for the synthesis of nitrogen-containing E-β-chlorovinyl sulfides based on the reaction of 2-alkynylamines with three equivalents of methanesulfonyl chloride in the presence of stoichiometric amounts of niobium(V) chloride and magnesium metal in toluene.
The Evaluation of Multiple Linear Regression–Based Limited Sampling Strategies for Mycophenolic Acid in Children with Nephrotic Syndrome
Authors: Sobiak Joanna , Resztak Matylda , Chrzanowska Maria , Zachwieja Jacek , Ostalska-Nowicka Danuta
Published: June 2021

We evaluated mycophenolic acid (MPA) limited sampling strategies (LSSs) established using multiple linear regression (MLR) in children with nephrotic syndrome treated with mycophenolate mofetil (MMF). MLR-LSS is an easy-to-determine approach of therapeutic drug monitoring (TDM). We assessed the practicability of different LSSs for the estimation of MPA exposure as well as the optimal time points for MPA TDM. The literature search returned 29 studies dated 1998–2020. We applied 53 LSSs (n = 48 for MPA, n = 5 for free MPA [fMPA]) to predict the area under the time-concentration curve (AUCpred) in 24 children with nephrotic syndrome, for whom we previously determined MPA and fMPA concentrations, and compare the results with the determined AUC (AUCtotal). Nine equations met the requirements for bias and precision ±15%. The MPA AUC in children with nephrotic syndrome was predicted the best by four time-point LSSs developed for renal transplant recipients. Out of five LSSs evaluated for fMPA, none fulfilled the ±15% criteria for bias and precision probably due to very high percentage of bound MPA (99.64%). MPA LSS for children with nephrotic syndrome should include blood samples collected 1 h, 2 h and near the second MPA maximum concentration. MPA concentrations determined with the high performance liquid chromatography after multiplying by 1.175 may be used in LSSs based on MPA concentrations determined with the immunoassay technique. MPA LSS may facilitate TDM in the case of MMF, however, more studies on fMPA LSS are required for children with nephrotic syndrome.
Synthesis of Hafnium(IV) Polyaminoacetates
Authors: Shulyak Alexandra T. , Bortnikov Evgeniy O. , Kubasov Alexey S. , Selivanov Nikita A. , Lipengolts Alexey A. , Zhdanov Andrey P. , Bykov Alexander Yu. , Zhizhin Konstantin Yu. , Kuznetsov Nikolai T.
Published: June 2021

The interaction of hafnium(IV) salts (oxide-dichloride, chloride, and bromide) with nitrilotriacetic acid (NTA), diethylenetriamminepentaacetic acid (DTPA), 1,2-diaminocyclohexanetetraacetic acid (CDTA), 1,3-dipropylmino-2-hydroxy N,N,N′,N′-tetraacetic acid (dpta), and N-(2-hydroxyethyl)ethylenediamine triacetic acid (HEDTA) has been studied. The corresponding complexes Na2[Hf(NTA)2]·3H2O (1), Na[HfDTPA]·3H2O (2), [HfCDTA(H2O)2] (3), and Na[Hf2(dpta)2]·7.5H2O·0.5C2H5OH (4) have been isolated and characterized and their structures have been determined by single crystal X-ray diffraction. Biological studies of [HfCDTA(H2O)2] have shown that in 5% glucose solution this complex has low toxicity and good contrasting ability.
A Regioselective Synthesis of Novel Functionalized Organochalcogen Compounds by Chalcogenocyclofunctionalization Reactions Based on Chalcogen Halides and Natural Products
Authors: Musalov Maxim V. , Potapov Vladimir A. , Yakimov Vladimir A. , Musalova Maria V. , Maylyan Arkady A. , Zinchenko Sergey V. , Amosova Svetlana V.
Published: June 2021

The regioselective synthesis of novel functionalized condensed organochalcogen compounds by chalcogenocyclofunctionalization reactions based on chalcogen halides and the natural products thymol and carvacrol has been developed. The reactions of selenium dibromide with allyl thymol and allyl carvacrol proceeded in methylene chloride at room temperature in the presence of NaHCO3 affording bis[(7-isopropyl-4-methyl-2,3-dihydro-1-benzofuran-2-yl)methyl] and bis[(4-isopropyl-7-methyl-2,3-dihydro-1-benzofuran-2-yl)methyl] selenides in 90–92% yield. Similar sulfides were obtained in 70–72% yields by the reaction of sulfur dichloride in chloroform under reflux. Trihalotellanes containing the same organic moieties were synthesized from allyl thymol, allyl carvacrol and tellurium tetrachloride or tetrabromide in quantitative yields. Corresponding functionalized ditellurides were prepared in 91–92% yields by the reduction of the trichlorotellanes with sodium metabisulfite in two-phase solvent system. The comparison of reactivity of sulfur, selenium and tellurium halides in chalcogenocyclofunctionalization and distinguishing features of each reaction were discussed.
Axisymmetric Hybrid Plasma Model for Hall Effect Thrusters
Authors: Panelli Mario , Morfei Davide , Milo Beniamino , D’Aniello Francesco Antonio , Battista Francesco
Published: June 2021

Hall Effect Thrusters (HETs) are nowadays widely used for satellite applications because of their efficiency and robustness compared to other electric propulsion devices. Computational modelling of plasma in HETs is interesting for several reasons: it can be used to predict thrusters’ operative life; moreover, it provides a better understanding of the physical behaviour of this device and can be used to optimize the next generation of thrusters. In this work, the discharge within the accelerating channel and near-plume of HETs has been modelled by means of an axisymmetric hybrid approach: a set of fluid equations for electrons has been solved to get electron temperatures, plasma potential and the discharge current, whereas a Particle-In-Cell (PIC) sub-model has been developed to capture the behaviour of neutrals and ions. A two-region electron mobility model has been incorporated. It includes electron–neutral/ion collisions and uses empirical constants, that vary as a continuous function of axial coordinates, to take into account electron–wall collisions and Bohm diffusion/SEE effects. An SPT-100 thruster has been selected for the verification of the model because of the availability of reliable numerical and experimental data. The results of the presented simulations show that the code is able to describe plasma discharge reproducing, with consistency, the physics within the accelerating channel of HETs. A small discrepancy in the experimental magnitude of ions’ expansion, due probably to boundary condition effects, has been found.
Protective Effect of Piplartine against LPS-Induced Sepsis through Attenuating the MAPKs/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation
Authors: Huang Chi-Han , Wang Shu-Chi , Chen I-Chen , Chen Yi-Ting , Liu Po-Len , Fang Shih-Hua , Huang Shu-Pin , Yeh Hsin-Chih , Liu Ching-Chih , Lee Po-Yen , Lin Tzu-Chieh , Cheng Wei-Chung , Su Chia-Cheng , Wu Hsin-En , Chen Yuan-Ru , Li Chia-Yang
Published: June 2021

Piplartine (or Piperlongumine) is a natural alkaloid isolated from Piper longum L., which has been proposed to exhibit various biological properties such as anti-inflammatory effects; however, the effect of piplartine on sepsis has not been examined. This study was performed to examine the anti-inflammatory activities of piplartine in vitro, ex vivo and in vivo using murine J774A.1 macrophage cell line, peritoneal macrophages, bone marrow-derived macrophages and an animal sepsis model. The results demonstrated that piplartine suppresses iNOS and COX-2 expression, reduces PGE2, TNF-α and IL-6 production, decreases the phosphorylation of MAPKs and NF-κB and attenuates NF-κB activity by LPS-activated macrophages. Piplartine also inhibits IL-1β production and suppresses NLRP3 inflammasome activation by LPS/ATP- and LPS/nigericin-activated macrophages. Moreover, piplartine reduces the production of nitric oxide (NO) and TNF-α, IL-6 and IL-1β, decreases LPS-induced tissue damage, attenuates infiltration of inflammatory cells and enhances the survival rate. Collectively, these results demonstrate piplartine exhibits anti-inflammatory activities in LPS-induced inflammation and sepsis and suggest that piplartine might have benefits for sepsis treatment.
The Danish Network for Community Pharmacy Practice Research and Development
Authors: Burghle Alaa , Hansen Rikke , Nørgaard Lotte , Hedegaard Ulla , Bendixen Susanne , Søndergaard Lone , Servilieri Kerly , Hansen Julianne , Rossing Charlotte
Published: June 2021

The community pharmacy has a number of attributes that makes it an excellent setting for research and development projects, as it is a highly accessible part of the healthcare system and is staffed by highly trained health care professionals. The big turnover in patients in the community pharmacy makes it possible to reach a great number of patients and collect a lot of data in a relatively short time. However, conducting nation-wide research and development projects can be a rather time-consuming process for the individual community pharmacy, and can thus require collaboration with other community pharmacies and researchers. This will help ensure strong results and better implementation. Thus, the Danish Network for Community Pharmacy Practice for Research and Development (NUAP) was established in Denmark by a number of highly committed community pharmacies and researchers. NUAP consists of 102 member pharmacy owners in addition to a number of researchers. The aim of the network is to strengthen pharmacy practice and pharmacy practice research in Denmark by providing a forum where community pharmacy practitioners and researchers meet and work together. The network is led by a steering committee elected by the members in the network.
Towards a Joint International Database: Alignment of SSR Marker Data for European Collections of Cherry Germplasm
Authors: Ordidge Matthew , Litthauer Suzanne , Venison Edward , Blouin-Delmas Marine , Fernandez-Fernandez Felicidad , Höfer Monika , Kägi Christina , Kellerhals Markus , Marchese Annalisa , Mariette Stephanie , Nybom Hilde , Giovannini Daniela
Published: June 2021

The objective of our study was the alignment of microsatellite or simple sequence repeat (SSR) marker data across germplasm collections of cherry within Europe. Through the European Cooperative program for Plant Genetic Resources ECPGR, a number of European germplasm collections had previously been analysed using standard sets of SSR loci. However, until now these datasets remained unaligned. We used a combination of standard reference genotypes and ad-hoc selections to compile a central dataset representing as many alleles as possible from national datasets produced in France, Great Britain, Germany, Italy, Sweden and Switzerland. Through the comparison of alleles called in data from replicated samples we were able to create a series of alignment factors, supported across 448 different allele calls, that allowed us to align a dataset of 2241 SSR profiles from six countries. The proportion of allele comparisons that were either in agreement with the alignment factor or confounded by null alleles ranged from 67% to 100% and this was further improved by the inclusion of a series of allele-specific adjustments. The aligned dataset allowed us to identify groups of previously unknown matching accessions and to identify and resolve a number of errors in the prior datasets. The combined and aligned dataset represents a significant step forward in the co-ordinated management of field collections of cherry in Europe.
Baicalein 5,6-Dimethyl Ether Prevents Memory Deficits in the Scopolamine Zebrafish Model by Regulating Cholinergic and Antioxidant Systems
Authors: Brinza Ion , Ayoub Iriny M. , Eldahshan Omayma A. , Hritcu Lucian
Published: June 2021

Baicalein 5,6-dimethyl ether, a bioactive flavonoid isolated for the first time from Alnus rugosa, was explored for its capability to relieve memory deficits and decrease oxidative stress. We examined the neuropharmacological effects of baicalein 5,6-dimethyl ether on scopolamine (Sco)-induced zebrafish (Danio rerio) anxiety, amnesia, and brain oxidative stress and attempted to elucidate the underlying mechanisms. Anxiety-like behavior, exploratory behavior, and memory performance were measured using novel tank-diving test (NTT), Y-maze, and novel object recognition (NOR) tests. For 10 days, baicalein 5,6-dimethyl ether (1, 3, and 5 µg/L) was administered through immersion, whereas Sco (100 μM) was delivered 30 min before behavioral tests. Treatment with baicalein 5,6-dimethyl ether reduced anxiety and memory impairment, and increased exploratory behavior in specific tests, along with significant protection from neuronal oxidative stress in the brain tissue of Sco-treated zebrafish. Antioxidant and anti-acetylcholinesterase (AChE) activities of baicalein 5,6-dimethyl ether in the Sco-induced zebrafish were further confirmed using in vivo assays. In Sco-treated zebrafish, baicalein 5,6-dimethyl ether regulated cholinergic function by inhibiting AChE activity. Baicalein 5,6-dimethyl ether may be a promising candidate compound for treating anxiety and amnesia by restoring cholinergic activity and reducing brain oxidative stress, according to our findings.
Influence of Orchard Cultural Practices during the Productive Process of Cherries through Life Cycle Assessment
Authors: Gaspar Pedro Dinis , Godina Radu , Barrau Rui
Published: June 2021

This study describes the influence of orchard cultural practices during the productive process of cherries on the environmental impact in terms of energy, air, soil and water through a “farm to market” Life Cycle Assessment (LCA). The results were used to identify the orchard cultural practices that contribute significantly to the environmental impact and to find solutions to reduce those impacts, serving as best practices guide to improving the environmental performance and as benchmarks for other national and international cherry and fruit growers. Primary data for production, harvest and post-harvest periods were gathered experimentally. The openLCA 1.10.2 software and the ecoinvent 3.5 database were used for modelling. Test case scenarios are modelled to identify the influence of cultural practices in low and high cherry production campaigns depending on climatic conditions and consequently diseases and plagues. Moreover, results are compared with other studies, not only covering cherries but also other fruits. The energy consumption per hectare in the production phase is similar in test scenarios. The energy consumption of orchard cultural practices related to tractor use, fertilizers and fungicides application are the main hotspots in terms of global warming, freshwater ecotoxicity and eutrophication, and terrestrial acidification. The use of electric vehicles, change the warehouse location or redefine transportation routes can reduce this impact, along with the optimization of the cherry’s quantity transported in each trip. In addition, the use of plant protection products, fertilizers and herbicides with less environmental impact will contribute to this objective. For that, the use of agriculture and precision systems to predict the need for fertilizers (nutrients), herbicides and fungicides, the use of decision support systems to define the dates of cultural practices, as well as innovative and emerging food and by-products processing methods are suggested. Thus, this study identifies and quantifies the environmental impacts associated with the production system of cherries and their main hotspots. It provides a best-practices guide for sustainable solutions in orchard management that contributes to the competitiveness and sustainability of fruit companies.
Cleaner Approach for Atrazine Removal Using Recycling Biowaste/Waste in Permeable Barriers
Authors: Lago Ana , Silva Bruna , Tavares Teresa
Published: June 2021

This work addresses the rehabilitation of water contaminated with atrazine, entrapping it in a permeable and sustainable barrier designed with waste materials (sepiolite) and with biomaterials (cork and pine bark). Atrazine adsorption was assessed by kinetics and equilibrium assays and desorption was tested with different extraction solvents. Adsorbed atrazine was 100% recovered from sepiolite using 20% acetonitrile solution, while 40% acetonitrile was needed to leach it from cork (98%) and pine bark (94%). Continuous fixed-bed experiments using those sorbents as PRB were performed to evaluate atrazine removal for up-scale applications. The modified dose-response model properly described the breakthrough data. The highest adsorption capacity was achieved by sepiolite (23.3 (±0.8) mg/g), followed by pine bark (14.8 (±0.6) mg/g) and cork (13.0 (±0.9) mg/g). Recyclability of sorbents was evaluated by adsorption-desorption cycles. After two regenerations, sepiolite achieved 81% of atrazine removal, followed by pine with 78% and cork with 54%. Sepiolite had the best performance in terms of adsorption capacity/stability. SEM and FTIR analyses confirmed no significant differences in material morphology and structure. This study demonstrates that recycling waste/biowaste is a sustainable option for wastewater treatment, with waste valorization and environmental protection.
Immunological Response to SARS-CoV-2 Is Sustained by Vitamin D: A Case Presentation of One-Year Follow-Up
Authors: Luciani Filippo , Caroleo Maria Cristina , Cannataro Roberto , Mirra Davida , D’Agostino Bruno , Gallelli Luca , Cione Erika
Published: June 2021

Vitamin D is necessary for normal bone development and conservation. Moreover, it has extraskeletal effects, which play a pivotal role as a modulator of innate and adaptive immune responses. Many studies have highlighted the beneficial effect of vitamin D in protecting against acute respiratory viral infection, including COVID-19. Within this context, we described the effect of vitamin D supplementation in the immunological response to SARS-CoV-2 infection. Long-term IgG SARS-CoV-2 antibody responses were assessed in a cohort of twenty-two subjects diagnosed with COVID-19 by chemiluminescence assay (CLIA). Among them, a 61-year-old nurse undergoing vitamin D therapy showed a positive IgG response against SARS-CoV-2 nucleocapsid over nine months after infection, suggesting vitamin D played a role in modulating early antibody response against SARS-CoV-2. This result provides evidence of a positive effect of vitamin D on the decrease of functional humoral immunity.
Revising the Observation Satellite Scheduling Problem Based on Deep Reinforcement Learning
Authors: Huang Yixin , Mu Zhongcheng , Wu Shufan , Cui Benjie , Duan Yuxiao
Published: June 2021

Earth observation satellite task scheduling research plays a key role in space-based remote sensing services. An effective task scheduling strategy can maximize the utilization of satellite resources and obtain larger objective observation profits. In this paper, inspired by the success of deep reinforcement learning in optimization domains, the deep deterministic policy gradient algorithm is adopted to solve a time-continuous satellite task scheduling problem. Moreover, an improved graph-based minimum clique partition algorithm is proposed for preprocessing in the task clustering phase by considering the maximum task priority and the minimum observation slewing angle under constraint conditions. Experimental simulation results demonstrate that the deep reinforcement learning-based task scheduling method is feasible and performs much better than traditional metaheuristic optimization algorithms, especially in large-scale problems.
Sequential Parameter Estimation of Modal Dispersion Curves with an Adaptive Particle Filter in Shallow Water: Experimental Results
Authors: Liu Hong , Yang Kunde , Yang Qiulong
Published: June 2021

An adaptive particle filter method is presented for performing sequential geoacoustic estimation of a shallow water acoustic environment using the explosive sound sources. This approach treats environmental parameters and source–receiver distance as unknown random variables that evolve as the source moves. As a sequential estimation method, this approach reduces the expense of computation than genetic algorithm and yields results with the same accuracy. Comparing with standard Particle filter, proposed method can adjust control parameters to adapt to a rapidly changing environment. This approach is demonstrated on the shallow water sound propagation data which was collected during the ASIAEX 2001 experiment. The results indicate that the geoacoustic parameters are well estimated and source–receiver distance are also well determined.
Small Angle Scattering Intensity Measurement by an Improved Ocean Scheimpflug Lidar System
Authors: Zhang Hongwei , Zhang Yuanshuai , Li Ziwang , Liu Bingyi , Yin Bin , Wu Songhua
Published: June 2021

Quantification of the horizontal patterns of phytoplankton and the distribution of suspended particles across the sea’s surface has been greatly improved by traditional passive oceanic color remote sensing technology. Lidar technology has already been proven to be effective positive remote sensing technology to construct high-resolution bathymetry models. Lidar technology significantly improves our ability to model biogeochemical processes in the upper ocean and provides advanced concepts regarding the vertical distribution of suspended particles and oceanic optical properties. In this paper, we present a novel optical approach to measuring the scattering intensity and characteristics of suspended particles within small angles backwards and distinguish water medium with different attenuation coefficients by a laboratory demonstration of the ocean Scheimpflug lidar system. The approach allows the direct determination of the scattering intensity over a small angle at the backward direction (175.8~178.8°) with an angular resolution of 0.38. Corrections for the effects of refraction at the air-glass-water interface were demonstrated. The data production (initial width and width attenuation rate of the laser beam) of the ocean Scheimpflug lidar system were utilized to distinguish water with different algae concentrations. Application for the measurement of backward scattering intensity and laser beam width were explored in distances up to several meters with spatial resolutions of millimeter precision.
Intelligent Parameter Identification for Robot Servo Controller Based on Improved Integration Method
Authors: Li Ye , Wang Dazhi , Zhou Shuai , Wang Xian
Published: June 2021

With the rise of smart robots in the field of industrial automation, the motion control theory of the robot servo controller has become a research hotspot. The parameter mismatch of the controller will reduce the efficiency of the equipment and damage the equipment in serious cases. Compared to other parameters of servo controllers, the moment of inertia and friction viscous coefficient have a significant effect on the dynamic performance in motion control; furthermore, accurate real-time identification is essential for servo controller design. An improved integration method is proposed that increases the sampling period by redefining the update condition in this paper; it then expands the applied range of the classical method that is more suitable for the working characteristics of a robot servo controller and reducesthe speed quantization error generated by the encoder. Then, an optimization approach using the incremental probabilistic neural network with improved Gravitational Search Algorithm (IGSA-IPNN) is proposed to filter the speed error by a nonlinear process and provide more precise input for parameter identification. The identified inertia and friction coefficient areused for the PI parameter self-tuning of the speed loop. The experiments prove that the validity of the proposed method and, compared to the classical method, it is more accurate, stable and suitable for the robot servo controller.
Fabrication and Characterization of Non-Equilibrium Plasma-Treated PVDF Nanofiber Membrane-Based Sensors
Authors: Sultana Quazi Nahida , Khan Mujibur , Mahamud Rajib , Saadatzi Mohammadsadegh , Sultana Papia , Farouk Tanvir , Quirino Rafael , Banerjee Sourav
Published: June 2021

The effect of a self-pulsing non-equilibrium plasma discharge on piezoelectric PVDF nanofiber membrane was investigated. The plasma discharge was generated in air with a DC power source, with a discharge current of 0.012 mA, a nominal interelectrode separation of 1 mm, and discharge voltage of ~970 V. In a continuous fabrication process, the electrospinning method was used to generate thin nanofiber membrane with a flow rate of 0.7–1 mL h−1 and 25–27 kV voltage to obtain the nanofiber with high sensitivity and a higher degree of alignment and uniformity over a larger area. Plasma treatment was applied on both single layer and multi-layer (three layers) nanomembranes. In addition, simultaneously, the nanofiber membranes were heat-treated at a glass transition temperature (80–120 °C) and then underwent plasma treatment. Fourier-transform infrared (FTIR) spectroscopy showed that the area under the curve at 840 and 1272 cm−1 (β phase) increased due to the application of plasma and differential scanning calorimeter (DSC) indicated an increase in the degree of crystallinity. Finally, PVDF sensors were fabricated from the nanofibers and their piezoelectric properties were characterized. The results suggested that compared to the pristine samples the piezoelectric properties in the plasma and plasma-heat-treated sensors were enhanced by 70% and 85% respectively.