bluuebunny/crossref_metadata_2025 huggingface dataset:
Modification and Validation of the System Causability Scale Using AI-Based Therapeutic Recommendations for Urological Cancer Patients: A Basis for the Development of a Prospective Comparative Study
Authors: Rinderknecht Emily , von Winning Dominik , Kravchuk Anton , Schäfer Christof , Schnabel Marco J. , Siepmann Stephan , Mayr Roman , Grassinger Jochen , Goßler Christopher , Pohl Fabian , Siska Peter J. , Zeman Florian , Breyer Johannes , Schmelzer Anna , Gilfrich Christian , Brookman-May Sabine D. , Burger Maximilian , Haas Maximilian , May Matthias
Published: November 2024

The integration of artificial intelligence, particularly Large Language Models (LLMs), has the potential to significantly enhance therapeutic decision-making in clinical oncology. Initial studies across various disciplines have demonstrated that LLM-based treatment recommendations can rival those of multidisciplinary tumor boards (MTBs); however, such data are currently lacking for urological cancers. This preparatory study establishes a robust methodological foundation for the forthcoming CONCORDIA trial, including the validation of the System Causability Scale (SCS) and its modified version (mSCS), as well as the selection of LLMs for urological cancer treatment recommendations based on recommendations from ChatGPT-4 and an MTB for 40 urological cancer scenarios. Both scales demonstrated strong validity, reliability (all aggregated Cohen’s K > 0.74), and internal consistency (all Cronbach’s Alpha > 0.9), with the mSCS showing superior reliability, internal consistency, and clinical applicability (p < 0.01). Two Delphi processes were used to define the LLMs to be tested in the CONCORDIA study (ChatGPT-4 and Claude 3.5 Sonnet) and to establish the acceptable non-inferiority margin for LLM recommendations compared to MTB recommendations. The forthcoming ethics-approved and registered CONCORDIA non-inferiority trial will require 110 urological cancer scenarios, with an mSCS difference threshold of 0.15, a Bonferroni corrected alpha of 0.025, and a beta of 0.1. Blinded mSCS assessments of MTB recommendations will then be compared to those of the LLMs. In summary, this work establishes the necessary prerequisites prior to initiating the CONCORDIA study and validates a modified score with high applicability and reliability for this and future trials.
Bivalent Fears of Evaluation in Social Anxiety: Evaluation of an Extended Psychoevolutionary Model
Authors: Bates Glen W. , Apputhurai Pragalathan , Knowles Simon R.
Published: November 2024

Fears of negative evaluation (FNEs) and fears of positive evaluation (FPEs) comprise a bivalent model of evaluation that can explain the aetiology and maintenance of Social Anxiety Disorder (SAD). In this study, we examined an extended version of this model which incorporates two related cognitive processes (concerns about reprisal and discounting of positive outcomes) as partial mediators of the effects of FNEs and FPEs. We built on earlier work by including a broader measure of social anxiety across different social situations and comparing models for groups of participants with and without probable SAD. Structural equation modelling was utilised to test the model in a sample of 890 university students (74.8% female, mean age 29.49). We replicated the findings of Cook et al. in the overall sample and in the group with probable SAD. FNEs and FPEs predicted social anxiety directly and were serially mediated by concerns about reprisal and discounting positive outcomes. The model was also a good fit for those without SAD; however, in the model, FNEs were no longer a direct predictor of social anxiety. The findings confirm the utility of the extended bivalent model and have implications for psychoevolutionary accounts of social anxiety.
Changes in the Global Structure of Energy Consumption and the Energy Transition Process
Authors: Gałecka Agnieszka , Pyra Mariusz
Published: November 2024

The global energy transition represents a pivotal aspect of the pursuit of sustainable development and the reduction of greenhouse gas emissions. The objective of this study was to examine the dynamic relationships between global primary energy consumption and the consumption of individual energy sources (nuclear, oil, coal, natural gas, renewables) from 2011 to 2023. To examine both long-run and short-run relationships between variables, advanced econometric methods were employed, including the Johansen cointegration test and the Vector Error Correction Model (VECM). Furthermore, an Index of Sustainable Energy Transformation (ISTE) was devised to quantify the advancement of the transition to low-carbon energy sources. The analysis confirms the existence of long-term equilibrium relationships between global primary energy consumption and the consumption of individual energy sources. The analysis revealed that renewable energy consumption exerts a considerable influence on primary energy consumption, both in the short and long term. The ISTE index demonstrated a notable increase over the period of 2011 to 2023, indicative of advancement in the global energy transition. The results confirm the existence of a stable long-term equilibrium between global primary energy consumption and the consumption of individual energy sources. The observed increase in the ISTE index indicates progress towards low-carbon energy sources, which has important implications for energy policy and sustainable development. The results can support policymakers in monitoring the progress of the energy transition and shaping policies to accelerate the development of renewable energy sources.
The Role of Productization in End-To-End Traceability
Authors: Harkonen Janne , Guerrero Rodriguez Javier Mauricio , Mustonen Erno
Published: November 2024

End-to-end traceability offers significant opportunities for product lifecycle visibility, sustainability enhancement, and regulatory compliance in product management. However, it faces challenges in data integration and management, supplier collaboration, cost and complexity, and the sharing of information across the supply chain. Productization refers to the representation of a product and connects commercial and technical aspects to the systemic perspective of product management. This includes a focus on the engineering lifecycle with inherent linkages to product data. The product management perspective, specifically in relation to the connection between end-to-end traceability and the productization concept, has not been extensively studied. This study explores the role of both productization and traceability in the context of end-to-end traceability. It combines an extensive literature review and an empirical example of applying productization logic across company borders to support end-to-end traceability. The key findings indicate that productization logic with a product structure focus can support end-to-end traceability in product management by providing consistency and a foundation for tracking both technical and operational data across the engineering lifecycle of a product. By focusing on productization, companies can overcome traceability challenges and unlock the benefits of end-to-end traceability.
Assessment of Public Transportation Safety Measures in Yaoundé, Cameroon: Case of Collective Taxis
Authors: Seunda Ekwe Idris Karel , Fondzenyuy Stephen Kome , Feudjio Tezong Steffel Ludivin , Wounba Jean François , Usami Davide Shingo , Persia Luca
Published: November 2024

Yaoundé, the capital of Cameroon, is one of the cities in the country most affected by road traffic crashes. Despite the measures taken by authorities, the human factor remains a major cause of these crashes. This study aimed to evaluate the measures taken to reduce the risk-taking behaviors of collective taxi drivers in Yaoundé. A survey of 144 collective taxi drivers was conducted to gather information on their driving habits, adherence to, and perceived effects of safety regulations. The study revealed the following prevalence of risky driving behaviors among collective taxi drivers: 41.33% for impaired driving; 67% for speeding, 62% for disobeying traffic lights, 68.86% for distraction; and 67% for risky maneuvering on the road. Significant associations were found between risk perceptions and involvement in risky driving behaviors. Associations were also established between the frequency of police inspections and involvement in risky behaviors, between the participation in training programs on safety issues and using poorly maintained vehicles, and between the frequency of awareness campaigns and poor maneuvering on the road. To address these issues, it is essential to strengthen preventive measures on risk factors, raise awareness on a large scale and on a regular basis, and strictly enforce the existing regulations.
The Development of a 3D Magnetic Field Scanner Using Additive Technologies
Authors: Sobko Artem , Yudanov Nikolai , Panina Larissa V. , Rodionova Valeriya
Published: November 2024

Visualizing magnetic fields is essential for studying the operation of electromagnetic systems and devices that use permanent magnets or magnetic particles. However, commercial devices for this purpose are often expensive due to their complex designs, which may not always be necessary for specific research needs. This work presents a method for designing an automated laboratory setup for magnetic cartography, utilizing a 3D printer to produce structural plastic components for the scanner. The assembly process is thoroughly described, covering both the hardware and software aspects. Spatial resolution and mapping parameters, such as the number of data points and the collection time, were configured through software. Multiple tests were conducted on samples featuring flat inductive coils on a printed circuit board, providing a reliable model for comparing calculated and measured results. The scanner offers several advantages, including a straightforward design, readily available materials and components, a large scanning area (100 mm × 100 mm × 100 mm), a user-friendly interface, and adaptability for specific tasks. Additionally, the integration of a pre-built macro enables connection to any PC running Windows, while the open-source microcontroller code allows users to customize the scanner’s functionality to meet their specific requirements.
Development of a Method for Evaluating H2-Filling Stations
Authors: Nolte Bastian , Gollnick Dominik , Stein Armin , Vietor Thomas
Published: November 2024

To expedite the development of the infrastructural expansion for hydrogen applications, the research project “THEWA” was founded. Within this project, the development of hydrogen-refueling stations is being advanced so that the hydrogen strategy for mobility in Germany can move forward. One development point of the project is to develop an evaluation model that recommends a concept for hydrogen-refueling stations for initial individual situations. In this work, an evaluation method is developed that provides an appropriate recommendation. For this purpose, basics, such as the general structure of hydrogen-refueling stations, their classification into functional areas, and already-existing evaluation methods for multi-criteria decisions, are shown. The method for the evaluation of hydrogen-refueling stations will be developed in a component-based manner, for which a selection of influencing factors of hydrogen-refueling stations will be explained and categorized. With the help of an expert workshop, these are scaled so that the result is an evaluation method based on an expert assessment and the consideration of individual customer requirements. In addition, the method is implemented in a tool so that it can be used more easily.
Therapeutic Challenges Derived from the Interaction Among Apolipoprotein E, Cholesterol, and Amyloid in Alzheimer’s Disease
Authors: Menendez-Gonzalez Manuel
Published: November 2024

The isoform E4 of the Apolipoprotein E (ApoE) represents one of the strongest genetic risk factors for late-onset Alzheimer’s disease (AD). ApoE has key roles in cholesterol transport and amyloid-β (Aβ) metabolism, which are both central to AD pathogenesis. The E4 isoform has been implicated in reduced cholesterol homeostasis, increased Aβ aggregation, and heightened tau phosphorylation, contributing to amyloid plaques and neurodegeneration. This manuscript examines the complex interactions among ApoE isoforms, cholesterol metabolism, and amyloid pathology. Moreover, the therapeutic challenges associated with lipid-lowering agents (e.g., statins, PCSK9 inhibitors), anti-amyloid immunotherapies, and anticoagulants are described, focusing on ApoE4 carriers. Decision-making challenges are discussed by analyzing the pros and cons of these therapies.
Oxidative Stress and Histomorphometric Remodeling: Two Key Intestinal Features of Type 2 Diabetes in Goto–Kakizaki Rats
Authors: Esteves-Monteiro Marisa , Ferreira-Duarte Mariana , Vitorino-Oliveira Cláudia , Costa-Pires José , Oliveira Sara , Matafome Paulo , Morato Manuela , Dias-Pereira Patrícia , Costa Vera Marisa , Duarte-Araújo Margarida
Published: November 2024

Gastrointestinal complications of diabetes are often overlooked, despite affecting up to 75% of patients. This study innovatively explores local glutathione levels and morphometric changes in the gut of Goto–Kakizaki (GK) rats, a type 2 diabetes animal model. Segments of the intestine, cecum, and colon were collected for histopathological analysis and glutathione quantification. A significant increase in the total thickness of the intestinal wall of GK rats was observed, particularly in the duodenum (1089.02 ± 39.19 vs. 864.19 ± 37.17 µm), ileum (726.29 ± 24.75 vs. 498.76 ± 16.86 µm), cecum (642.24 ± 34.15 vs. 500.97 ± 28.81 µm), and distal colon (1211.81 ± 51.32 vs. 831.71 ± 53.2 µm). Additionally, diabetic rats exhibited thickening of the muscular layers in all segments, except for the duodenum, which was also the only portion where the number of smooth muscle cells did not decrease. Moreover, myenteric neuronal density was lower in GK rats, suggesting neurological loss. Total glutathione levels were lower in all intestinal segments of diabetic rats (except duodenum), and the reduced/oxidized glutathione ratio (GSH/GSSG) was significantly decreased in GK rats, indicating increased oxidative stress. These findings strongly indicate that GK rats undergo significant intestinal remodeling, notable shifts in neuronal populations, and heightened oxidative stress—factors that likely contribute to the functional gastrointestinal alterations seen in diabetic patients.
Underneath the Gut–Brain Axis in IBD—Evidence of the Non-Obvious
Authors: Boldyreva Lidiya V. , Evtushenko Anna A. , Lvova Maria N. , Morozova Ksenia N. , Kiseleva Elena V.
Published: November 2024

The gut–brain axis (GBA) plays a pivotal role in human health and wellness by orchestrating complex bidirectional regulation and influencing numerous critical processes within the body. Over the past decade, research has increasingly focused on the GBA in the context of inflammatory bowel disease (IBD). Beyond its well-documented effects on the GBA–enteric nervous system and vagus nerve dysregulation, and gut microbiota misbalance—IBD also leads to impairments in the metabolic and cellular functions: metabolic dysregulation, mitochondrial dysfunction, cationic transport, and cytoskeleton dysregulation. These systemic effects are currently underexplored in relation to the GBA; however, they are crucial for the nervous system cells’ functioning. This review summarizes the studies on the particular mechanisms of metabolic dysregulation, mitochondrial dysfunction, cationic transport, and cytoskeleton impairments in IBD. Understanding the involvement of these processes in the GBA may help find new therapeutic targets and develop systemic approaches to improve the quality of life in IBD patients.
Prolonged Photobiomodulation with Deep Red Light Mitigates Incipient Retinal Deterioration in a Mouse Model of Type 2 Diabetes
Authors: Opazo Gabriela , Tapia Felipe , Díaz Alejandra , Vielma Alex H. , Schmachtenberg Oliver
Published: November 2024

Diabetic retinopathy is a prevalent complication of type 2 diabetes mellitus, characterized by progressive damage to the retinal structure and function. Photobiomodulation therapy, using red or near-infrared light, has been proposed as a non-invasive intervention to mitigate retinal damage, but has been tested generally with short-term stimuli. This study aimed to evaluate the effects of prolonged photobiomodulation with deep red light on retinal structure and function in a type 2 diabetes mouse model. Transgenic LepRdb/J (db/db) mice were exposed to photobiomodulation therapy via LED devices emitting 654 nm light for 12 h daily over ten weeks and compared to untreated mice. Retinal function was evaluated by flash electroretinography, while structural changes were assessed through histology and immunohistochemistry to detect astro- and microgliosis. At 33 weeks of age, db/db mice were obese and severely diabetic, but exhibited only incipient indicators of retinal deterioration. Electroretinogram b-wave peak latencies were prolonged at intermediate flash intensities, while the outer plexiform layer displayed significantly elevated IBA1 expression. Photobiomodulation therapy prevented these two markers of early retinal deterioration but had no effect on other morphological and functional parameters. Photobiomodulation is well-tolerated and maintains potential as a complementary treatment option for diabetic retinopathy but requires further optimization of therapeutic settings and combinatory treatment approaches.
Gene Therapy Approach for Treatment of Obese Agouti Mice
Authors: Yunin Maxim A. , Boychenko Stanislav S. , Lebedev Petr , Deykin Alexey V. , Pokrovskii Mikhail V. , Egorov Alexander D.
Published: November 2024

Obesity is a significant metabolic disorder associated with excessive fat accumulation and insulin resistance. In this study, we explored a gene therapy approach to treat obesity in agouti mice using adeno-associated viruses (AAVs) carrying PRDM16, FoxP4, or Follistatin (FST) genes, which are known to promote the browning of white adipose tissue. Mice treated with AAVs encoding PRDM16, FoxP4, or FST genes showed a reduction in body weight (10–14%) within the first three weeks after administration, compared to the control groups. A lipidomic analysis of the adipose tissue revealed a dramatic reduction in triacylglycerol (TAG) species with low carbon numbers (40–54 acyl carbons) in treated mice.
Malware Classification Using Few-Shot Learning Approach
Authors: Alfarsi Khalid , Rasheed Saim , Ahmad Iftikhar
Published: November 2024

Malware detection, targeting the microarchitecture of processors, has recently come to light as a potentially effective way to improve computer system security. Hardware Performance Counter data are used by machine learning algorithms in security mechanisms, such as hardware-based malware detection, to categorize and detect malware. It is crucial to determine whether or not a file contains malware. Many issues have been brought about by the rise in malware, and businesses are losing vital data and dealing with other issues. The second thing to keep in mind is that malware can quickly cause a lot of damage to a system by slowing it down and encrypting a large amount of data on a personal computer. This study provides extensive details on a flexible framework related to machine learning and deep learning techniques using few-shot learning. Malware detection is possible using DT, RF, LR, SVM, and FSL techniques. The logic is that these algorithms make it simple to differentiate between files that are malware-free and those that are not. This indicates that their goal is to reduce the number of false positives in the data. For this, we use two different datasets from an online platform. In this research work, we mainly focus on few-shot learning techniques by using two different datasets. The proposed model has an 97% accuracy rate, which is much greater than that of other techniques.
The Malvaceae Collection at the Rio de Janeiro Botanical Garden: Ex Situ Conservation to Meet Target 8 of the Global Strategy for Plant Conservation
Authors: Ferreira Carlos Daniel Miranda , de Mattos Juliana Ribeiro , Coelho Marcus Alberto Nadruz , Bovini Massimo G.
Published: November 2024

Ex situ conservation of plants is a current and urgent issue, especially in the Brazilian context. While Brazil has the world’s highest plant diversity, few consistent initiatives are aimed at conserving the potential of our living collections toward reaching Target 8 of the Global Strategy for Plant Conservation (GSPC). Objective II of GSPC calls for the conservation of plant diversity, with Target 8 specifying 75% of threatened plant species in ex situ collections. It was only after cataloging the collection of Malvaceae from the Rio de Janeiro Botanical Garden (JBRJ) for scientific publication that we realized the potential of this collection for ex situ conservation. With this in mind, we carefully catalog the individuals present, updating the names of species and counting their individuals. We found that Malvaceae is represented by 63 species and 216 individuals in the arboretum, 45 species native to America, 11 from Africa, 10 from Asia, and 3 from Oceania. Using IUCN criteria, only two species are threatened and two are data-deficient, with one or two individuals each. Based on these data and the specific biology of this taxonomic group, we identified the main problems and listed recommendations to make this collection more representative of the endangered taxa of the Brazilian flora. Therefore, we expect this effort to be a solid contribution to Target 8 mandated by GSPC, as well as a replicable pilot project for other taxonomic groups of Brazilian flora.
Effects of Titanate on Brake Wear Particle Emission Using a Brake Material Friction Test Dynamometer
Authors: Daimon Emiko , Ito Yasuhito
Published: November 2024

We investigated the effect of lepidocrocite-type layered titanate, which is compounded in brake pads, to reduce brake particle emissions. The dust reduction effect of titanate was evaluated using a small-scale inertial brake material friction test dynamometer. The results suggested that brake particle emissions are related to the microphysical structure of the pad surface, such as the uniformity of the friction film and secondary plateau formation, and that friction materials containing titanate contribute significantly to reducing both particle mass (PM) and particle number (PN) emissions of brake particles in both non-asbestos organic (NAO) and low-steel (LS) pads. In particular, LS pads generally have a problem of having more brake particles than NAO pads, but this study found that brake particles can be significantly reduced by compounding titanate instead of tin sulfide.
Application of Product of Vitrification of Asbestos-Cement Waste and CRT Glass Cullet as Reinforcing Phase in Surface Composites Produced by FSP Method
Authors: Iwaszko Józef , Kudła Krzysztof , Lubas Małgorzata
Published: November 2024

In this study, the vitrification of asbestos-cement waste (ACW) and glass cullet from cathode-ray tubes (CRTs) was performed. The resulting product of vitrification from the abovementioned waste was used as the reinforcing phase in a composite with the AA7075 alloy matrix. The composite was made by means of the FSP (friction stir processing) method. The main aim of this work was to determine whether the product of the vitrification can be utilized as the reinforcing phase in the composite. The tests show that introducing the vitrification product into the composite matrix increases both the hardness of the material and its wear resistance. The composite was characterized by a 39% higher hardness and 30.4% higher wear resistance compared to the initial AA7075 alloy. The changes in the properties were caused by strong refinement of the grains, but primarily by the presence of the hard particles of the reinforcing phase in the composite matrix. This research demonstrates that vitrified material, thanks to its properties, can constitute a full-value reinforcing material that can ultimately replace more expensive engineering materials in composites.
An Assessment of the Strength and Physical Properties of Edible Tableware from Flax Seed and Flaxseed Cake
Authors: Andrejko Dariusz , Blicharz-Kania Agata
Published: November 2024

Alternatives to traditional disposable plastic tableware are constantly sought. The aim of the study was to assess the possibility of using oilseeds and their press cakes for the production of edible tableware. Edible vegan plates (P) and bowls (B) were produced. The basic ingredients used for production were flax seeds (S) or flax press cake (C). Plates made using press cakes under a pressure of 3 kg deformed to a lesser extent than those containing seeds. However, they were more susceptible to crumbling during shaking. The colour of the tableware made on the basis of flax press cakes was lighter and was characterised by a higher chromaticity in the yellow and red direction. Significantly higher water absorption was characteristic of the tableware in which flax press cakes were used instead of flax seeds. The lowest water absorption (17.14%) after 30 min of soaking was recorded for the PS sample. After the test simulating the use of the tableware, a significant reduction in strength was observed overall (except for the PS test). The panelists rated the consistency and palatability of the PS, BS and PC tests very similarly. The highest overall acceptability was noted for the BS and PC tests. In conclusion, the development of edible bowls and plates made from flax seeds or flaxseed cake is an alternative solution for the production of environmentally friendly tableware.
Determining the Permeability of Porous Bioceramic Scaffolds: Significance, Overview of Current Methods and Challenges Ahead
Authors: Gabrieli Roberta , Schiavi Alessandro , Baino Francesco
Published: November 2024

The “architectural suitability” of scaffolds for bone tissue engineering is commonly evaluated by assessing the pore volume and the mean pore size (or pore size distribution, if possible) and comparing these values with the reference ranges of human cancellous bone. However, these two parameters cannot precisely describe the complex architecture of bone scaffolds and just provide a preliminary comparative criterion. Permeability is suggested as a more comprehensive and significant parameter to characterize scaffold architecture and mass transport capability, being also related to bone in-growth and, thus, functional properties. However, assessing the permeability of bioactive ceramics and glass scaffolds is a complex task from both methodological and experimental viewpoints. After providing an overview of the fundamentals about porosity in scaffolds, this review explores the different experimental and numerical approaches used to determine the permeability of porous bioceramics, describing the methodologies used (pump-based, gravity-based, acoustic and computational methods) and highlighting advantages and limitations to overcome (e.g., reliability issues and need for better standardization of the experimental procedures).
Microscopic Analysis and Evaluation of Thermal Elevation and Wear of Drills for Implant Site Preparation: An In Vitro Study
Authors: Memè Lucia , Bambini Fabrizio , Pizzolante Tommaso , Principi Martina , Sampalmieri Francesco , Mummolo Stefano
Published: November 2024

Drilling for implant site preparation generates heat, which can cause bone necrosis if temperatures exceed 47 °C for over a minute. Factors influencing heat include drill size, speed, pressure, irrigation, and tool wear. Frequent drill replacement is essential, as wear from repeated use and sterilization affects performance. This study compared three pilot drills with similar designs from different manufacturers, testing each on pig ribs for 15 perforations after 15 sterilization cycles. Researchers measured temperature increase, drilling time, and surface wear. Results showed that drill no. 1 generated more heat than drills no. 2 and no. 3, though none reached critical temperatures. Drill no. 2 took the longest to reach the desired depth and displayed the most deformation. Findings highlight the importance of adhering to the recommended operational limits, suggesting that drills should be replaced after 15 cycles to ensure efficacy and patient safety.
Salmon Nasal Cartilage-Derived Proteoglycans Alleviate Monosodium Iodoacetate-Induced Osteoarthritis in Rats
Authors: Jeong Inae , Park Jinbum , Park Shinjung , Wada Tatuya , Lim Dae Soo , Kim Ok-Kyung
Published: November 2024

Osteoarthritis is a chronic inflammatory condition characterized by the degeneration of joint cartilage and underlying bone, resulting in pain, swelling, and reduced mobility. This study evaluates the efficacy of salmon nasal cartilage-derived proteoglycans in mitigating osteoarthritis symptoms and investigates the underlying molecular mechanisms. This study employed a rat model of osteoarthritis induced by monosodium iodoacetate (MIA) injection. The rats were orally administered salmon nasal cartilage-derived proteoglycans or ibuprofen. Key aspects of osteoarthritis pathology, including impaired exercise ability, inflammation, extracellular matrix degradation, and chondrocyte apoptosis, were assessed using histological analysis, micro-CT, treadmill testing, serum assays, and mRNA/protein expression studies. The MIA injection caused significant cartilage damage, reduced bone mineral density, and impaired exercise ability. Additionally, it elevated serum levels of prostaglandin E2 and nitric oxide, increased the mRNA and protein levels of inflammation-related factors, and activated apoptosis signaling pathways in cartilage. Treatment with salmon nasal cartilage-derived proteoglycans significantly improved cartilage morphology and mineralization, reduced inflammation, and inhibited apoptosis signaling pathways, with effects comparable to those observed with ibuprofen treatment. These findings highlight the potential of salmon nasal cartilage-derived proteoglycans as a therapeutic agent for managing osteoarthritis by effectively reducing inflammation, preventing cartilage degradation, and inhibiting chondrocyte apoptosis.
State of Play in the Implementation of the Principles of Inclusive Leadership in Fintech Companies in Lithuania
Authors: Budreikaitė Justina , Rapuano Violeta , Raišienė Agota Giedrė
Published: November 2024

The multifaceted challenges posed by globalization, medical advancements, conflicts and crises necessitate a critical examination of social and organizational inclusion. Despite the acknowledged advantages of inclusive leadership (IL), there remains a significant research gap concerning the perceptions of IL among diverse employee groups, particularly within heterogeneous organizations. This study aims to bridge this gap by exploring the perceptions of IL across various sociodemographic groups within fintech companies in Lithuania. By concentrating on this rapidly evolving sector, this research enhances the understanding of IL by assessing how sociodemographic variables influence perceptions of inclusive leadership practices. A total of 236 responses were analyzed using the Mann–Whitney U and Kruskal–Wallis tests, supplemented by the Bonferroni correction to ensure statistical robustness. The findings elucidate the dynamics of IL within the fast-paced fintech environment, revealing unique challenges and opportunities to foster organizational inclusivity. The implications of this research provide actionable insights for industry leaders striving to implement IL strategies that empower minority groups and enhance overall organizational performance. Although the results indicate that fintech organizations have made strides in embedding IL principles, these advancements are not uniformly experienced across diverse employee demographics. Specifically, this study identifies significant disparities in IL perceptions between employees of other genders and those with health-related impairments. Acknowledging certain limitations, particularly the small sample sizes of some examined employee subgroups, this study advocates for further research to yield generalizable findings that can inform and improve inclusive organizational practices.
Rapid Synthesis of Non-Toxic, Water-Stable Carbon Dots Using Microwave Irradiation
Authors: Soledad-Flores Olga , Bailón-Ruiz Sonia J. , Román-Velázquez Félix
Published: November 2024

Carbon dots (C-Dots) have garnered significant attention in various fields, including biomedical applications, photocatalysis, sensing, and optoelectronics, due to their high luminescence, biocompatibility, and ease of functionalization. However, concerns regarding their potential toxicity persist. Conventional synthesis methods for C-Dots often require long reaction times, high pressures, expensive equipment, extreme temperatures, and toxic reagents. In contrast, microwave irradiation provides a rapid, cost-effective, and scalable alternative for the synthesis of high-quality C-Dots. In this study, we report the single-step, 3-min synthesis of water-stable carbon dots at 100 °C, 120 °C, and 140 °C using microwave irradiation. Particle stability was achieved through polyethyleneimine (PEI) functionalization. The toxicity of the synthesized carbon dots was evaluated in marine crustaceans, revealing that C-Dots with an estimated size below 10 nm did not exhibit toxicity after 24 and 48 h of exposure. These findings demonstrate the potential of microwave-synthesized carbon dots as non-toxic, water-stable nanomaterials for environmental and biomedical applications.
Quantitative Analysis of Amorphous Form in Indomethacin by Near Infrared Spectroscopy Combined with Partial Least Squares Regression Analysis
Authors: Liu Mingdi , Fu Rui , Liu Jichao , Song Ping , Li Haichao , Dong Weibing , Sun Zan
Published: November 2024

Indomethacin (INDO) is a synthetic non-steroidal antipyretic, analgesic, and anti-inflammatory drug that commonly exists in both amorphous and crystalline states. Its amorphous state (A-INDO) is utilized by pharmaceutical companies as an active pharmaceutical ingredient (API) in the production of INDO drugs due to its higher apparent solubility and bioavailability. The crystal state also encompasses various crystal forms such as the α-crystal form (α-INDO) and γ-crystal form (γ-INDO), with the highly crystalline and insoluble γ-INDO being commercially available. A-INDO, existing in a thermodynamically high-energy state, is susceptible to several factors during the preparation, storage, and transportation of API leading to its conversion into γ-INDO, thus impacting the bioavailability and efficacy of INDO drugs. Therefore, quantitative analysis of the A-INDO/γ-INDO content in INDO API becomes essential for controlling the production quality of INDO. The primary objective of this study is to investigate the feasibility of NIR for the quantitative analysis of A-INDO in INDO API, and to further elucidate its quantitative analysis mechanism. The NIR spectral data were collected for A-INDO and γ-INDO binary mixture samples with different resolutions, and these spectra were then selected and reconstructed using the interval partial least square (iPLS) method. Different pretreatment methods were employed to enhance the reconstructed spectra by highlighting relevant eigen information while eliminating invalid information caused by environmental factors or physical characteristics of samples. The most suitable PLSR model for quantitative analysis of A-INDO within the range of 0.0000–100.0000% w/w% was established, screened, and validated. From various perspectives, including distribution of spectral effective information, impact of resolution on PLSR model performance, variance contribution/cumulative variance contribution of PLSR model principal components (PCs), PCI loadings, relationship between spectral scores, and A-INDO content, feasibility assessment was conducted for the quantitative analysis of A-INDO in INDO using NIR spectroscopy. Additionally, a detailed investigation on the quantitative analysis mechanism of the optimal PLSR model was undertaken including the correlation between the characteristic peaks of spectra and information regarding hydrogen groups or hydrogen bonds in A-INDO or γ-INDO molecules. This study aims to provide theoretical support for the quantitative analysis of A-INDO in INDO API as well as serve as a reliable reference method for API quantification and quality control in similar drugs.
Engineering pH and Temperature-Triggered Drug Release with Metal-Organic Frameworks and Fatty Acids
Authors: Wei Wanying , Lu Ping
Published: November 2024

This study reports the successful synthesis of core-shell microparticles utilizing coaxial electrospray techniques, with zeolitic imidazolate framework-8 (ZIF-8) encapsulating rhodamine B (RhB) in the core and a phase change material (PCM) shell composed of a eutectic mixture of lauric acid (LA) and stearic acid (SA). ZIF-8 is well-recognized for its pH-responsive degradation and biocompatibility, making it an ideal candidate for targeted drug delivery. The LA-SA PCM mixture, with a melting point near physiological temperature (39 °C), enables temperature-triggered drug release, enhancing therapeutic precision. The structural properties of the microparticles were extensively characterized through scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Drug release studies revealed a dual-stimuli response, where the release of RhB was significantly influenced by both temperature and pH. Under mildly acidic conditions (pH 4.0) at 40 °C, a rapid and complete release of RhB was observed within 120 h, while at 37 °C, the release rate was notably slower. Specifically, the release at 40 °C was 79% higher than at 37 °C, confirming the temperature sensitivity of the system. Moreover, at physiological pH (7.4), minimal drug release occurred, demonstrating the system’s potential for minimizing premature drug release under neutral conditions. This dual-stimuli approach holds promise for improving therapeutic outcomes in cancer treatment by enabling precise control over drug release in response to both pH and localized hyperthermia, reducing off-target effects and improving patient compliance.
The Identification of Six Estrogen Preparations by Combining Thin-Layer Chromatography with Micro-Raman Imaging Spectroscopy
Authors: Zhu Wenquan , Liang Xin , Li Mengjiao , Wang Xinrui , Cui Hongxia , Dong Yan , Bu Hongzhou , Dong Wei , Sui Huimin , Xu Feng , Fang Yuhui , Ma Chunhui
Published: November 2024

A method for identifying six estrogen preparations by thin-layer chromatography combined with Raman imaging microscopy (TLC-RIM) was established. An appropriate pretreatment method was adopted to extract and purify the six estrogen preparations. After that, each estrogen extraction solution was spotted on a thin-layer chromatography plate. Estriol (E3), estradiol (E2), estradiol valerate (EV), estradiol benzoate (EB), nilestriol (CEE), and ethinylestradiol (EE2) were separated by TLC, and their Rf value and localization were determined under a UV lamp at 254 nm, followed by the in situ enrichment of the drug component. Using a 532 nm laser as the light source, the Raman scattering spectrum of the component was directly collected by micro-Raman imaging. The Rf values after TLC separation of the six estrogens and their Raman spectra can, respectively, reflect differences in polarity and structure, and they are not affected by the excipients of preparation. The detection limits of the six estrogens are 0.636, 1.00, 0.687, 0.497, 0.649, and 0.626 mg/mL. Based on the intensity of the minimum characteristic peak, the stability results within 40 min showed that the RSD of each substance is 1.34, 2.06, 1.65, 3.99, 1.16, and 2.71%, respectively. This method has strong specificity, good stability, and high sensitivity, and it can provide a new reference for improving the identification standards of estrogen preparations.
pH-Responsive Metal–Organic Framework for Targeted Delivery of Fungicide, Release Behavior, and Sustainable Plant Protection
Authors: Yang Shuzhen , Lü Fulin , Wang Li , Liu Sinan , Wu Zhisai , Cheng Yanqin , Liu Fan
Published: November 2024

A smart and environmentally friendly pesticide system was developed that could respond to environmental stimuli while mitigating environmental risks. In this study, thiabendazole (Thi), an effective fungicide, was loaded onto zeolitic imidazolate framework-8 (ZIF-8) using the impregnation method to fabricate a pH-responsive nano hybrid delivery system (Thi@ZIF-8). The results demonstrated that Thi@ZIF-8 had a rhombic dodecahedral morphology and a loading capacity of approximately 25%. Notably, the amount of Thi released from Thi@ZIF-8 at a pH of 5.0 reached 79.54%, which was higher than that at pH 7.0 and 9.0, for 251 h. Such pH-responsive release characteristics of Thi@ZIF-8 were probably related to the pH-dependent structure stability of ZIF-8. The release mechanism of Thi@ZIF-8 conformed to non-Fickian diffusion. Additionally, Thi@ZIF-8 showed a higher control efficacy against B. cinerea compared with Thi alone. Importantly, the ZIF-8 carrier could effectively reduce the leaching loss of Thi in soil and showed no negative effects on the three varieties of tomato seedlings, implying good biocompatibility. This work provides a novel and eco-friendly approach to control B. cinerea effectively that has great potential in modern sustainable agriculture.
Advances in Organic Materials for Next-Generation Optoelectronics: Potential and Challenges
Authors: Nowsherwan Ghazi Aman , Ali Qasim , Ali Umar Farooq , Ahmad Muhammad , Khan Mohsin , Hussain Syed Sajjad
Published: November 2024

This review provides a comprehensive overview of recent advancements in the synthesis, properties, and applications of organic materials in the optoelectronics sector. The study emphasizes the critical role of organic materials in the development of state-of-the-art optoelectronic devices such as organic solar cells, organic thin-film transistors, and OLEDs. The review further examines the structure, operational principles, and performance metrics of organic optoelectronic devices. Organic materials have emerged as promising candidates due to their low-cost production and potential for large-area or flexible substrate applications. Additionally, this review highlights the physical mechanisms governing the optoelectronic properties of high-performance organic materials, particularly photoinduced processes relevant to charge carrier photogeneration. It discusses the unique benefits of organic materials over traditional inorganic materials, including their light weight, simple processing, and flexibility. The report delves into the challenges related to stability, scalability, and performance, while highlighting the wide range of electronic properties exhibited by organic materials, which are critical for their performances in optoelectronic devices. Furthermore, it addresses the need for further research and development in this field to achieve consistent performance across different types of devices.
Film Properties of Heparin Cross-Linked with Epichlorohydrin in Absence or Presence of Imidazole
Authors: Šimkovic Ivan , Gucmann Filip , Hricovíni Michal , Mendichi Raniero , Dobročka Edmund , Schieroni Alberto Giacometti , Piovani Daniele , Zappia Stefania , Hricovíni Miloš
Published: November 2024

We cross-linked unfractionated heparin (H) using epichlorohydrin (E), in the absence or presence of imidazole (I), using various ratios of H, E, and I substances. The objectives and goals were to use the reaction for the preparation of medical materials suitable for blood sample applications. Nuclear magnetic resonance indicated the involvement of an H-end sequence [H-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl-α-Ser] in the linkage with the 2-hydroxypropyl bridge. The yields of the individual experiments were found to increase in the following ratios: 1H/1E/3I (24%) < 1H/1E/2I (32%) < 1H/3E (42%) < 1H/1E/1I (46%) < 1H/2E (64%) < 1H/1E (77%). According to size-exclusion chromatography with multiple-angle light scattering (SEC-MALS) analysis, the mass at the peak increased in the following order: H (9292 g/mol) < 1H/1E (9294 g/mol) < 1H/2E (9326 g/mol) < 1H/3E (9708 g/mol) < 1H/1E/2I (11,212 g/mol) < 1H/1E/3I (12,301 g/mol) < 1H/1E/1I (13,800 g/mol) and in the reverse order with the increase in amount of epichlorohydrin and imidazole, i.e., 1H/1E > 1H/2E > 1H/3E and 1H/1E/1I > 1H/1E/2I > 1H/1E/3I. X-ray diffraction revealed that all prepared films were amorphous. An evaluation of the surface morphology using atomic force microscopy (AFM) confirmed a relatively low films roughness (~0.9–3.6 nm). The surface reduced elastic modulus, determined by the PeakForce quantitative nanomechanical mapping (PF-QNM) technique, was found to increase by up to ~63% for films cross-linked with E in the absence of I when compared with the results for the H substrate. A negligible change in modulus was, however, observed for films cross-linked in the presence of I, or was even reduced by ~15% (1H/1E/3I) compared to that for the H substrate. This could be explained by the parallel cross-linking of H only with E within its serine end unit and in competition with only one nitrogen of I. According to the highest yield (77%) of 1H/1E, the preferred product is the following: H-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl-α-Ser-CH2-CH(OH)-CH2-OH. For the 1H/1E/1I (46% yield), 1H/1E/2I (32%), and 1H/1E/3I (24%) products, the cross-linked motif was the same, and the difference represented the surplus amount of the imidazolium cation ionically bound to the heparin anionic groups.
Gelation and Cryogelation of Chitosan: Origin of Low Efficiency of Diglycidyl Ethers as Cross-Linkers in Acetic Acid Solutions
Authors: Privar Yuliya , Skatova Anna , Golikov Alexey , Boroda Andrey , Bratskaya Svetlana
Published: November 2024

Although diglycidyl ethers of glycols (DEs)—FDA-approved reagents for biomedical applications—were considered unsuitable for the fabrication of chitosan (CH) hydrogels and cryogels, we have recently shown that CH cross-linking with DEs is possible, but its efficiency depends on the nature of the acid used to dissolve chitosan and pH. To elucidate the origin of the low efficiency of chitosan interactions with DEs in acetic acid solutions, we have put forward two hypotheses: (i) DEs are consumed in a side reaction with acetic acid; (ii) DE chain length strongly affects the probability of cross-linking. We then verified them using FT-IR spectroscopy, rheological measurements, and uniaxial compression tests. The formation of esters in acetic acid solutions was confirmed for ethylene glycol diglycidyl ether (EGDE) and poly(ethylene glycol) diglycidyl ether (PEGDE). By the 7th day of gelation at pH 5.5, the G’HCl/G’HAc ratio was 5.1 and 1.5 for EGDE and PEGDE, respectively, indicating that the loss of cross-linking efficiency in acetic acid solution was less pronounced for the long-chain cross-linker. Under conditions of cryotropic gelation, only weak cryogels were obtained from acetic acid solutions at a DE:CH molar ratio of 1:1, while stable cryogels were fabricated at a molar ratio of 1:20 from HCl solutions.