bluuebunny/crossref_metadata_2025 huggingface dataset:
The normal impact stiffness of a debris-flow flexible barrier
Authors: Huo Miao , Zhou Jia-wen , Zhao Jiangtao , Zhou Hong-wei , Li Jidong , Liu Xing
Published: March 2023

This paper proposes a normal oriented impact stiffness of a three-supporting cable flexible barrier under a small pretension stress to estimate the structural load behaviour, and employs two categories of small-scale debris flows (coarse and fine) to explore the stiffness evolution through physical model experiments with high-speed photography and load sensing. Results suggest that the particle-structure contact is essential to the normal load effect. Coarse debris flow performs more frequent particle-structure contact and exerts evident momentum flux, while fine debris flows with few physical collisions impart much smaller one. The middle-sited cable that receives only tensile force from vertical equivalent cable-net joint system exhibits indirect load behaviour. The bottom-sited cable shows high load feedback due to the sum of direct contact of debris flow and tensile forces. The relationship between impact loads and maximum cable deflections can be explained by power functions according to quasi-static theory. The impact stiffness is not just affected by the particle-structure contact but by the flow inertia and particle collision effect. Savage number Nsav and Bagnold number Nbag manage to depict the dynamical effects on the normal stiffness Di. Experiments indicate that Nsav has positive linear correlation with the nondimensionalization of Di, whilst Nbag has positive power correlation with the nondimensionalization of Di. This idea is an alternative scope for the study on flow-structure interaction and may contribute to the parameter identification in numerical simulation of the debris flow-structure interaction and the optimization of the design standardization.
Albedo effects in the ER3BP with an oblate primary, a triaxial secondary and a potential due to belt
Authors: Singh Jagadish , Richard Tyokyaa K.
Published: March 2023

We have examined the effects of Albedo in the Elliptic Restricted Three-Body Problem (ER3BP) with an oblate primary, a triaxial secondary, and potential due to belt for the Earth–Moon system. We have found that as the perturbed parameters increases, the possible boundary regions of the primary come closer to one other, allowing particles to travel from one region to the next freely and possibly merge the permissible regions. Our study has revealed that the formation of triangular libration points depends on the Albedo effects, semi-major axis, the Eccentricity of the orbits, triaxiality, and the potential due to the belt. As the parameters mentioned above increase, the triangular positions $${L}_{4}$$

L
4

and $${L}_{5}$$

L
5

move towards the center of origin in cases 1, 2, 3, and 4 and away from the center of the origin in cases 5, 6, and 7. Considering the range of a stable and unstable libration point for the problem under study given as $$0<\mu <{\mu }_{c}$$

0
<
μ
<

μ
c

for stable libration points and $${\mu }_{c}\leq\mu \le \frac{1}{2}$$

μ
c

≤
μ
≤

1
2

for unstable libration points, our study has established that the triangular libration points are respectively stable and unstable for cases 1, 2, and 6 and cases 3, 4, 5, and 7. Our study has also revealed that each set of values has at least one characteristic complex root with a positive real part. Hence, the triangular libration points for the Earth–Moon system are unstable in the sense of Lyapunov. The Earth–Moon system's Poincare Surface of Section (PSS) has demonstrated that a slight change in the initial conditions, the semi-major axis, and the Eccentricity of the orbits have affected the system's behavior dramatically. Further, it is seen that a chaotic dynamical behavior of the system results into either regular or irregular orbits.
An integrative survival analysis and a systematic review of the cerebellopontine angle glioblastomas
Authors: Lasica Nebojsa , Arnautovic Kenan , Tadanori Tomita , Vulekovic Petar , Kozic Dusko
Published: March 2023

Glioblastomas presenting topographically at the cerebellopontine angle (CPA) are exceedingly rare. Given the specific anatomical considerations and their rarity, overall survival (OS) and management are not discussed in detail. The authors performed an integrative survival analysis of CPA glioblastomas. A literature search of PubMed, Scopus, and Web of Science databases was performed per PRISMA guidelines. Patient data including demographics, clinical features, neuroimaging, management, follow-up, and OS were extracted. The mean age was 39 ± 26.2 years. The mean OS was 8.9 months. Kaplan–Meier log-rank test and univariate Cox proportional-hazards model identified hydrocephalus (log-rank, p = 0.034; HR 0.34; 95% CI 0.12–0.94; p = 0.038), chemotherapy (log-rank, p < 0.005; HR 5.66; 95% CI 1.53–20.88; p = 0.009), and radiotherapy (log-rank, p < 0.0001; HR 12.01; 95% CI 3.44–41.89; p < 0.001) as factors influencing OS. Hydrocephalus (HR 3.57; 95% CI 1.07–11.1; p = 0.038) and no adjuvant radiotherapy (HR 0.12; 95% CI 0.02–0.59; p < 0.01) remained prognostic on multivariable analysis with fourfold and twofold higher risk for the time-related onset of death, respectively. This should be considered when assessing the risk-to-benefit ratio for patients undergoing surgery for CPA glioblastoma.
The quality of vital signs measurements and value preferences in electronic medical records varies by hospital, specialty, and patient demographics
Authors: Jackson Niall , Woods Jessica , Watkinson Peter , Brent Andrew , Peto Tim E. A. , Walker A. Sarah , Eyre David W.
Published: March 2023

We aimed to assess the frequency of value preferences in recording of vital signs in electronic healthcare records (EHRs) and associated patient and hospital factors. We used EHR data from Oxford University Hospitals, UK, between 01-January-2016 and 30-June-2019 and a maximum likelihood estimator to determine the prevalence of value preferences in measurements of systolic and diastolic blood pressure (SBP/DBP), heart rate (HR) (readings ending in zero), respiratory rate (multiples of 2 or 4), and temperature (readings of 36.0 °C). We used multivariable logistic regression to investigate associations between value preferences and patient age, sex, ethnicity, deprivation, comorbidities, calendar time, hour of day, days into admission, hospital, day of week and speciality. In 4,375,654 records from 135,173 patients, there was an excess of temperature readings of 36.0 °C above that expected from the underlying distribution that affected 11.3% (95% CI 10.6–12.1%) of measurements, i.e. these observations were likely inappropriately recorded as 36.0 °C instead of the true value. SBP, DBP and HR were rounded to the nearest 10 in 2.2% (1.4–2.8%) and 2.0% (1.3–5.1%) and 2.4% (1.7–3.1%) of measurements. RR was also more commonly recorded as multiples of 2. BP digit preference and an excess of temperature recordings of 36.0 °C were more common in older and male patients, as length of stay increased, following a previous normal set of vital signs and typically more common in medical vs. surgical specialities. Differences were seen between hospitals, however, digit preference reduced over calendar time. Vital signs may not always be accurately documented, and this may vary by patient groups and hospital settings. Allowances and adjustments may be needed in delivering care to patients and in observational analyses and predictive tools using these factors as outcomes or exposures.
Safety level assessment of shield tunneling in water rich sandy pebble strata with large particle size
Authors: Yao Peiyi , Yu Ziwang , Lv Yan , Shi Bin , He Yuanyuan , Wang Hong , Liu Danna , Wei Shengda
Published: March 2023

Shield tunneling method is widely used in urban metro construction. The construction stability is closely related to the engineering geological conditions. Sandy pebble strata have a loose structure and low cohesion, resulting in great engineering-induced stratigraphic disturbance. Meanwhile, the high water-abundance and strong permeability are extremely detrimental to construction safety. It is of great significance to evaluate the dangerousness of shield tunneling in water-rich pebble strata with large particle size. In this paper, risk assessment of engineering practice is carried through with Chengdu metro project in China as a case study. Referring to the special engineering situations and assessment workload, seven evaluation indices, including compressive strength of pebble layer, boulder volume content, permeability coefficient, groundwater depth, grouting pressure, tunneling speed and tunnel buried depth are selected to establish an evaluation system. A complete risk assessment framework is established based on the cloud model, AHP and entropy weight method. Further, the measured surface settlement is taken as the risk degree characterization to verify the results. This study can provide reference for method selection and evaluation system establishment in the risk assessment of shield tunnel construction in water-rich sandy pebble strata, and contribute to proposing safety management in similar engineering projects.
Climate, landscape, and life history jointly predict multidecadal community mosquito phenology
Authors: Campbell Lindsay P. , Sallam Mohamed F. , Bauer Amely M. , Tavares Yasmin , Guralnick Robert P.
Published: March 2023

Phenology of adult host-seeking female mosquitoes is a critical component for understanding potential for vector-borne pathogen maintenance and amplification in the natural environment. Despite this importance, long-term multi-species investigations of mosquito phenologies across environments and differing species’ life history traits are rare. Here we leverage long-term mosquito control district monitoring data to characterize annual phenologies of 7 host-seeking female mosquito species over a 20-year time period in suburban Illinois, USA. We also assembled data on landscape context, categorized into low and medium development, climate variables including precipitation, temperature and humidity, and key life history traits, i.e. overwintering stage and Spring–Summer versus Summer–mid-Fallseason fliers. We then fit linear mixed models separately for adult onset, peak abundances, and flight termination with landscape, climate and trait variables as predictors with species as a random effect. Model results supported some expectations, including warmer spring temperatures leading to earlier onset, warmer temperatures and lower humidity leading to earlier peak abundances, and warmer and wetter fall conditions leading to later termination. However, we also found sometimes complex interactions and responses contrary to our predictions. For example, temperature had generally weak support on its own, impacting onset and peak abundance timing; rather temperature has interacting effects with humidity or precipitation. We also found higher spring precipitation, especially in low development contexts, generally delayed adult onset, counter to expectations. These results emphasize the need to consider how traits, landscape and climatic factors all interact to determine mosquito phenology, when planning management strategies for vector control and public health protection.
The extent of windfarm infrastructures on recognised European blanket bogs
Authors: Chico Guaduneth , Clewer T. , Midgley N. G. , Gallego-Anex P. , Ramil-Rego P. , Ferreiro J. , Whayman E. , Goeckeritz S. , Stanton T.
Published: March 2023

Peatland environments are the Earth’s largest terrestrial carbon store and have the potential to act as carbon sinks. However, the development of windfarms on peatlands is affecting their morphology, hydrology, ground-level climate conditions, carbon functions and vegetation, and long-term consequences still need to be assessed. Blanket bogs are a rare type of ombrotrophic peatland that are typical of oceanic areas with high precipitation and low temperatures. Their distribution has been mapped across Europe, where they are mainly located on hill summits where wind energy potential is higher, making them attractive sites for windfarm developments. The promotion of renewable energy is currently a priority given the environmental and economic drive to increase low-carbon energy production. Establishing windfarms on peatland in pursuit of greener energy, therefore, risks compromising and undermining the green-energy transition. Despite this, the extent of windfarm infrastructures on blanket bogs have not yet been reported at the European scale. This research reports the extent of windfarm infrastructures on recognised blanket bogs, with a geographical focus on Europe, where blanket bogs have been mapped systematically. Under the EU Habitats Directive (92/43/EEC), there are 36 European regions NUTS level 2 with recognised blanket bogs. Of these, 12 have windfarm developments, including 644 wind turbines, 253.4 km of vehicular access tracks and an affected area of 207.6 ha, mainly in Ireland and Scotland where the extent of blanket bogs is also higher. However, despite Spain having under 0.2% of Europe’s recognised blanket bogs area, this was the most affected country. In Scotland, a comparison of the recognised blanket bogs under the Habitats Directive (92/43/EEC) with blanket bogs recorded in national inventories indicates that the extent of windfarm developments was higher, with 1,063 wind turbines and 634.5 km of vehicular access tracks. Our results highlight the extent of windfarm developments on blanket bog habitat, both in areas where peatlands are broadly distributed across the landscape, and also in areas where this recognised habitat is particularly rare. There is a pressing need to assess the long-term impacts of windfarms on peatlands to ensure that efforts to meet energy targets result only in carbon sequestration, and do not jeopardise ecosystem services. Blanket bogs represent a particularly vulnerable habitat, the study of which should be prioritised updating national and international inventories to protect and restore this habitat.
Disease characteristics of idiopathic transverse myelitis with serum neuronal and astroglial damage biomarkers
Authors: Kim Keon-Woo , Lee Eun-Jae , Kim Sang-Yeob , Jung Hee-Jae , Kim Hyo Jae , Kim Seungmi , Kim Hyunji , Seo Dayoung , So Jungmin , Kim Jiyon , Kim Hyunjin , Kim Kwang-Kuk , Lim Young-Min
Published: March 2023

Despite its close association with CNS inflammatory demyelinating disorders (CIDDs), pathogenic characteristics of idiopathic transverse myelitis (ITM) remain largely unknown. Here, we investigated serum levels of neurofilament light chain (sNfL) and glial fibrillary acidic protein (sGFAP) in patients with ITM to unravel the disease characteristics of ITM. We prospectively recruited 70 patients with ITM, 62 with AQP4 + NMOSD and 85 with RRMS—including 31 patients with acute TM attacks—along with 30 HCs. We measured sNfL and sGFAP levels using single-molecular arrays and compared these levels per lesion volume between the disease groups during attacks. Compared to HCs, ITM patients showed higher sNfL and sGFAP during acute attacks (sNfL: p < 0.001, sGFAP: p = 0.024), while those in remission (sNfL: p = 0.944, sGFAP: p > 0.999) did not, regardless of lesion extents and presence of multiple attacks. ITM patients demonstrated lower sGFAP/volume (p = 0.011) during acute attacks and lower sGFAP (p < 0.001) in remission compared to AQP4 + NMOSD patients. These findings suggest that both neuronal and astroglial damages occur in patients with acute ITM attacks at a similar level to those with RRMS, distinct from AQP4 + NMOSD. However, active neuroinflammatory process was not remarkable during remission in this cohort.
Grain boundary widening controls siderite (FeCO3) replacement of limestone (CaCO3)
Authors: Weber Juliane , Starchenko Vitalii , Ilavsky Jan , Allard Lawrence F. , Mata Jitendra , Debeer-Schmitt Lisa , Cooke Carolyn Grace , Littrell Ken , He Lilin , Zhang Rui , Stack Andrew G. , Anovitz Lawrence M.
Published: March 2023

The microstructure of minerals and rocks can significantly alter reaction rates. This study focuses on identifying transport paths in low porosity rocks based on the hypothesis that grain boundary widening accelerates reactions in which one mineral is replaced by another (replacement reaction). We conducted a time series of replacement experiments of three limestones (CaCO3) of different microstructures and solid impurity contents using FeCl2. Reacted solids were analyzed using chemical imaging, small angle X-ray and neutron scattering and Raman spectroscopy. In high porosity limestones replacement is reaction controlled and complete replacement was observed within 2 days. In low porosity limestones that contain 1–2% dolomite impurities and are dominated by grain boundaries, a reaction rim was observed whose width did not change with reaction time. Siderite (FeCO3) nucleation was observed in all parts of the rock cores indicating the percolation of the solution throughout the complete core. Dolomite impurities were identified to act as nucleation sites leading to growth of crystals that exert force on the CaCO3 grains. Widening of grain boundaries beyond what is expected based on dissolution and thermal grain expansion was observed in the low porosity marble containing dolomite impurities. This leads to a self-perpetuating cycle of grain boundary widening and reaction acceleration instead of reaction front propagation.
Assessment of long-term large deformation in deep roadways due to roof fracturing impact loading
Authors: Huang Wanpeng , Golsanami Naser , Zhang Chengguo , Canbulat Ismet , Xin Guangming , Sun Gang , Jiang Lishuai
Published: March 2023

The rock mass around deep roadways has obvious creep characteristics in high-stress environments. Meanwhile, the cyclic impact load induced by roof fracturing also causes dynamic damage to the surrounding rock, leading to long-term large deformation. This paper examined the rock mass deformation mechanism around deep roadways based on the theory of rock creep perturbation effect considering perturbation sensitive zone. This study proposed a long-term stability control guideline for deep roadways under dynamic load. An innovative support system was developed for deep roadways, with concrete-filled steel tubular support being recommended as the main supporting body. A case study was conducted to validate the proposed supporting system. Monitoring over one year in the case study mine showed that the overall convergence deformation of the roadway was 35 mm, indicating that the roadway’s long-term large deformation induced by creep perturbation was effectively controlled by using the proposed bearing circle support system.
Development of a predictive model to identify patients most likely to benefit from surgery in metastatic breast cancer
Authors: Bai Jinfeng , Li Zeying , Guo Junlong , Gao Fuxin , Zhou Hui , Zhao Weijie , Ma Xiang
Published: March 2023

Primary tumor resection for metastatic breast cancer (MBC) has demonstrated a survival advantage, however, not all patients with MBC benefit from surgery. The purpose of this study was to develop a predictive model to select patients with MBC who are most likely to benefit from surgery at the primary site. Data from patients with MBC were obtained from the Surveillance, Epidemiology and End Results (SEER) cohort and patients treated at the Yunnan Cancer Hospital. The patients from the SEER database were divided into surgery and non-surgery groups and a 1:1 propensity score matching (PSM) was used to balance baseline characteristics. We hypothesized that patients who underwent local resection of primary tumors had improved overall survival (OS) compared to those who did not undergo surgery. Based on the median OS time of the non-surgery group, patients from the surgery group were further categorized into beneficial and non-beneficial groups. Logistic regression analysis was performed to identify independent factors associated with improved survival in the surgery group and a nomogram was established using the most significant predictive factors. Finally, internal and external validation of the prognostic nomogram was also evaluated by concordance index (C-index) and using a calibration curve. A total of 7759 eligible patients with MBC were identified in the SEER cohort and 92 with MBC patients who underwent surgery at the Yunnan Cancer Hospital. Amongst the SEER cohort, 3199 (41.23%) patients received surgery of the primary tumor. After PSM, the OS between the surgery and non-surgery group was significantly different based on Kaplan–Meier survival analysis (46 vs. 31 months, P < 0.001), In the surgery group, 562 (55.20%) patients survived for longer than 31 months and were classified in the beneficial group. Significant differences were observed in patient characteristics between the beneficial and non-beneficial groups including age, grade, tumor size, liver metastasis, breast cancer subtype and marital status. These factors were used as independent predictors to create a nomogram. The internally and externally validated C-indices of the nomogram were 0.703 and 0.733, respectively, indicating strong consistency between the actual and predicted survival. A nomogram was developed and used to identify MBC patients who are most likely to benefit from primary tumor resection. This predictive model has the potential to improve clinical decision-making and should be considered routine clinical practice.
Ferromagnetic order controlled by the magnetic interface of LaNiO3/La2/3Ca1/3MnO3 superlattices
Authors: Soltan S. , Macke S. , Ilse S. E. , Pennycook T. , Zhang Z. L. , Christiani G. , Benckiser E. , Schütz G. , Goering E.
Published: March 2023

Interface engineering in complex oxide superlattices is a growing field, enabling manipulation of the exceptional properties of these materials, and also providing access to new phases and emergent physical phenomena. Here we demonstrate how interfacial interactions can induce a complex charge and spin structure in a bulk paramagnetic material. We investigate a superlattice (SLs) consisting of paramagnetic LaNiO3 (LNO) and highly spin-polarized ferromagnetic La2/3Ca1/3MnO3 (LCMO), grown on SrTiO3 (001) substrate. We observed emerging magnetism in LNO through an exchange bias mechanism at the interfaces in X-ray resonant magnetic reflectivity. We find non-symmetric interface induced magnetization profiles in LNO and LCMO which we relate to a periodic complex charge and spin superstructure. High resolution scanning transmission electron microscopy images reveal that the upper and lower interfaces exhibit no significant structural variations. The different long range magnetic order emerging in LNO layers demonstrates the enormous potential of interfacial reconstruction as a tool for tailored electronic properties.
Low-carbohydrate diets containing plant-derived fat but not animal-derived fat ameliorate heart failure
Authors: Bujo Satoshi , Toko Haruhiro , Ito Kaoru , Koyama Satoshi , Ishizuka Masato , Umei Masahiko , Yanagisawa-Murakami Haruka , Guo Jiaxi , Zhai Bowen , Zhao Chunxia , Kishikawa Risa , Takeda Norifumi , Tsushima Kensuke , Ikeda Yuichi , Takimoto Eiki , Morita Hiroyuki , Harada Mutsuo , Komuro Issei
Published: March 2023

Cardiovascular disease (CVD) is a global health burden in the world. Although low-carbohydrate diets (LCDs) have beneficial effects on CVD risk, their preventive effects remain elusive. We investigated whether LCDs ameliorate heart failure (HF) using a murine model of pressure overload. LCD with plant-derived fat (LCD-P) ameliorated HF progression, whereas LCD with animal-derived fat (LCD-A) aggravated inflammation and cardiac dysfunction. In the hearts of LCD-P-fed mice but not LCD-A, fatty acid oxidation-related genes were highly expressed, and peroxisome proliferator-activated receptor α (PPARα), which regulates lipid metabolism and inflammation, was activated. Loss- and gain-of-function experiments indicated the critical roles of PPARα in preventing HF progression. Stearic acid, which was more abundant in the serum and heart of LCD-P-fed mice, activated PPARα in cultured cardiomyocytes. We highlight the importance of fat sources substituted for reduced carbohydrates in LCDs and suggest that the LCD-P-stearic acid-PPARα pathway as a therapeutic target for HF.
Waste cooking oil processing over cobalt aluminate nanoparticles for liquid biofuel hydrocarbons production
Authors: Ibrahim M. A. , El-Araby R. , Abdelkader Elham , Saied Mohamed El , Abdelsalam A. M. , Ismail E. H.
Published: March 2023

The catalytic conversion of waste cooking oil (WCO) was carried out over a synthetic nano catalyst of cobalt aluminate (CoAl2O4) to produce biofuel range fractions. A precipitation method was used to create a nanoparticle catalyst, which was then examined using field-emission scanning electron microscopy, X-ray diffraction, energy dispersive X-ray, nitrogen adsorption measurements, high-resolution transmission electron Microscopy (HRTEM), infrared spectroscopy, while a gas chromatography-mass spectrometer (GC–MS) was used to analyze the chemical construction of the liquid biofuel. A range of experimental temperatures was looked at including 350, 375, 400, 425, and 450 °C; hydrogen pressure of 50, 2.5, and 5.0 MPa; and liquid hour space velocity (LHSV) of 1, 2.5, and 5 h−1. As temperature, pressure, and liquid hourly space velocity increased, the amount of bio-jet and biodiesel fractional products decreased, while liquid light fraction hydrocarbons increased. 93% optimum conversion of waste cooking oil over CoAl2O4 nano-particles was achieved at 400 °C, 50 bar, and 1 h−1 (LHSV) as 20% yield of bio-jet range,16% gasoline, and 53% biodiesel. According to the product analysis, catalytic hydrocracking of WCO resulted in fuels with chemical and physical characteristics that were on par with those required for fuels derived from petroleum. The study's findings demonstrated the nano cobalt aluminate catalyst's high performance in a catalytic cracking process, which resulted in a WCO to biofuel conversion ratio that was greater than 90%. In this study, we looked at cobalt aluminate nanoparticles as a less complex and expensive alternative to traditional zeolite catalysts for the catalytic cracking process used to produce biofuel and thus can be manufactured locally, which saves the cost of imports for us as a developing country.
Medication adherence in patients with cluster headache and migraine: an online survey
Authors: Rimmele Florian , Müller Britta , Becker-Hingst Nadine , Wegener Sophia , Rimmele Stefanie , Kropp Peter , Jürgens Tim P.
Published: March 2023

To examine factors for adherent and non-adherent behavior in patients with cluster headache and migraine. Adults with cluster headache or migraine were included in this anonymous online survey using a questionnaire accessed via homepages of headache support groups. Medication adherence in preventive treatment was measured with the Medication Adherence Report Scale (MARS-D). Factors for non-adherent behavior were examined (subjective socioeconomic status, psychological comorbidities, self-efficacy, coping, side effects, expectations of treatment, information on medical treatment, and trust in the physician/treatment concept). 200 participants (n = 58 with cluster headache, n = 142 with migraine) were included. The rate of medication adherence in preventive treatment was 32.8% for participants with cluster headache and 20.4% for migraine. The most common reasons for low adherence in participants with cluster headache were altering the prescribed medication dose (34%) or taking less than instructed (14%), which was mostly due to insufficient benefit from the medication or side effects. Positive expectations of medical treatment (p ≤ 0.05) correlated significantly with adherent behavior in cluster headache. Furthermore, the adherence-promoting factors coping and self-efficacy were more pronounced in patients with cluster headache than in those with migraine (p < 0.05). This study is the first to comprehensively investigate medication adherence and factors influencing adherent/non-adherent behavior in patients with cluster headache. Patients with cluster headache had similar adherence levels to patients with migraine, but had higher resources of adherence-promoting factors.
Cognitive and plastic recurrent neural network clock model for the judgment of time and its variations
Authors: Hallez Quentin , Mermillod Martial , Droit-Volet Sylvie
Published: March 2023

The aim of this study in the field of computational neurosciences was to simulate and predict inter-individual variability in time judgements with different neuropsychological properties. We propose and test a Simple Recurrent Neural Network-based clock model that is able to account for inter-individual variability in time judgment by adding four new components into the clock system: the first relates to the plasticity of the neural system, the second to the attention allocated to time, the third to the memory of duration, and the fourth to the learning of duration by iteration. A simulation with this model explored its fit with participants’ time estimates in a temporal reproduction task undertaken by both children and adults, whose varied cognitive abilities were assessed with neuropsychological tests. The simulation successfully predicted 90% of temporal errors. Our Cognitive and Plastic RNN-Clock model (CP-RNN-Clock), that takes into account the interference arising from a clock system grounded in cognition, was thus validated.
Root exudate-derived compounds stimulate the phosphorus solubilizing ability of bacteria
Authors: Pantigoso Hugo A. , Manter Daniel K. , Fonte Steven J. , Vivanco Jorge M.
Published: March 2023

Low phosphorus (P) availability in soils is a major challenge for sustainable food production, as most soil P is often unavailable for plant uptake and effective strategies to access this P are limited. Certain soil occurring bacteria and root exudate-derived compounds that release P are in combination promising tools to develop applications that increase phosphorus use efficiency in crops. Here, we studied the ability of root exudate compounds (galactinol, threonine, and 4-hydroxybutyric acid) induced under low P conditions to stimulate the ability of bacteria to solubilize P. Galactinol, threonine, and 4-hydroxybutyric acid were incubated with the P solubilizing bacterial strainsEnterobacter cloacae,Pseudomonas pseudoalcaligenes, andBacillus thuringiensisunder either inorganic (calcium phosphate) or organic (phytin) forms of plant-unavailable P. Overall, we found that the addition of individual root exudate compounds did not support bacterial growth rates. However, root exudates supplemented to the different bacterial appeared to enhance P solubilizing activity and overall P availability. Threonine and 4-hydroxybutyric acid induced P solubilization in all three bacterial strains. Subsequent exogenous application of threonine to soils improved the root growth of corn, enhanced nitrogen and P concentrations in roots and increased available levels of potassium, calcium and magnesium in soils. Thus, it appears that threonine might promote the bacterial solubilization and plant-uptake of a variety of nutrients. Altogether, these findings expand on the function of exuded specialized compounds and propose alternative approaches to unlock existing phosphorus reservoirs of P in crop lands.
Mock community as an in situ positive control for amplicon sequencing of microbiotas from the same ecosystem
Authors: Galla Giulio , Praeg Nadine , Colla Filippo , Rzehak Theresa , Illmer Paul , Seeber Julia , Hauffe Heidi Christine
Published: March 2023

Metataxonomy has become the standard for characterizing the diversity and composition of microbial communities associated with multicellular organisms and their environment. Currently available protocols for metataxonomy assume a uniform DNA extraction, amplification and sequencing efficiency for all sample types and taxa. It has been suggested that the addition of a mock community (MC) to biological samples before the DNA extraction step could aid identification of technical biases during processing and support direct comparisons of microbiota composition, but the impact of MC on diversity estimates of samples is unknown. Here, large and small aliquots of pulverized bovine fecal samples were extracted with no, low or high doses of MC, characterized using standard Illumina technology for metataxonomics, and analysed with custom bioinformatic pipelines. We demonstrated that sample diversity estimates were distorted only if MC dose was high compared to sample mass (i.e. when MC > 10% of sample reads). We also showed that MC was an informative in situ positive control, permitting an estimation of the sample 16S copy number, and detecting sample outliers. We tested this approach on a range of sample types from a terrestrial ecosystem, including rhizosphere soil, whole invertebrates, and wild vertebrate fecal samples, and discuss possible clinical applications.
Hand hygiene practice and associated factors among rural communities in northwest Ethiopia
Authors: Gizaw Zemichael , Demissie Negesu Gizaw , Gebrehiwot Mulat , Destaw Bikes , Nigusie Adane
Published: March 2023

This community-based cross-sectional study was conducted among 1190 randomly selected rural households in northwest Ethiopia to assess hand hygiene practice and associated factors. Frequent handwashing with rubbing agents, drying mechanisms; and condition of fingernails were used to assess hand hygiene practice. Multivariable binary logistic regression analysis was used to identify factors associated with hand hygiene and statistically significant association was declared on the basis of adjusted odds ratio (AOR) with 95% confidence interval (CI) and p-values < 0.05. Results showed that 28.8% (95% CI 26.2, 31.4%) of the households had good hand hygiene practice. Good hand hygiene practice was significantly associated with formal education attended household heads (AOR 1.79, 95% CI 1.33, 2.40), family discussion on sanitation (AOR 1.56, 95% CI 1.08, 2.26), provision of health education (AOR 2.23, 95% CI 1.62, 3.06), and availability of water (AOR 3.51, 95% CI 1.02, 12.05). In conclusion, about one-third of the rural households had good hand hygiene practice and more than two-third had poor hand hygiene practice in the study area, and this may imply that hands in the area may play roles in spreading infections in the community. Therefore, people need to be informed to always keep their hand hygiene good.
Nigella sativa seeds mitigate the hepatic histo-architectural and ultrastructural changes induced by 4-nonylphenol in Clarias gariepinus
Authors: Abd-Elkareem Mahmoud , Sayed Alaa El-Din H. , Khalil Nasser S. Abou , Kotob Mohamed H.
Published: March 2023

Due to its prevalence in aquatic environments and potential cytotoxicity, 4-nonylphenol (4-NP) has garnered considerable attention. As a medicinal plant with numerous biological activities, Nigella sativa (black seed or black cumin) seed (NSS) is widely utilized throughout the world. Consequently, this study aimed to examine the potential protective effects of NSS against 4-NP-induced hepatotoxicity in African catfish (Clarias gariepinus). To achieve this objective, 18 fish (351 ± 3 g) were randomly divided into three equal groups for 21 days. The first group serves as a control which did not receive any treatment except the basal diet. The second and third groups were exposed to 4-NP at a dose of 0.1 mg L−1 of aquarium water and fed a basal diet only or supplemented with 2.5% NSS, respectively. The histological, histochemical, and ultrastructural features of the liver were subsequently evaluated as a damage biomarker of the hepatic tissue. Our results confirmed that 4-NP was a potent hepatotoxic agent, as 4-NP-intoxicated fish exhibited many lesions. Steatohepatitis, ballooning degeneration, sclerosing cholangitis, and coagulative necrosis of melanomacrophagecenters (MMCs) were observed. Hemosiderin, lipofuscin pigments, and proliferation of fibroblasts, kupffer cells, and telocytes were also demonstrated in the livers of 4-NP-intoxicated fish. In addition, decreased glycogen content and increased collagen deposition were observed in the hepatic tissue. Hepatocytes exhibited ultrastructural alterations in the chromatin, rough endoplasmic reticulum, smooth endoplasmic reticulum, mitochondria, lysosomes, and peroxisomes. Co-administration of 2.5% NSS to 4-NP-intoxicated fish significantly reduced these hepatotoxic effects. It nearly preserved the histological, histochemical, and ultrastructural integrity of hepatic tissue.
Intelligent computing with Levenberg–Marquardt artificial neural network for Carbon nanotubes-water between stretchable rotating disks
Authors: Ali Faizan , Awais Muhammad , Ali Aamir , Vrinceanu Narcisa , Shah Zahir , Tirth Vineet
Published: March 2023

Hybrid Nano fluid has emerged to be an important field of study due to its better thermal performance compared to other Nano fluids. The problem of carbon nanotubes rotating between two stretchable discs while suspended in water is investigated in this research. Due to numerous uses of this problem, such as metal mining, drawing plastic films, and cooling continuous filaments, this problem is essential to industry. Considerations here include suction/injection, heat radiation, and the Darcy-Forchheimer scheme with convective boundary conditions. The partial differential equations are reduced to ordinary differential equations by using appropriate transformation. To examine the approximate solution validation, training and testing procedures are interpreted and the performance is verified through error histogram and mean square error results. To describe the behavior of flow quantities, several tabular and graphical representations of a variety of physical characteristics of importance are presented and discussed in detail. The basic aim of this research is to examine the behaviour of carbon nanotubes (nanoparticles) between stretchable disks while considering the heat generation/absorption parameter by using the Levenberg–Marquardt technique of artificial neural network. Heat transfer rate is accelerated by a decrease in velocity and temperature and an increase in the nanoparticle volume fraction parameter which is a significant finding of the current study.
Preparing pathological data to develop an artificial intelligence model in the nonclinical study
Authors: Hwang Ji-Hee , Lim Minyoung , Han Gyeongjin , Park Heejin , Kim Yong-Bum , Park Jinseok , Jun Sang-Yeop , Lee Jaeku , Cho Jae-Woo
Published: March 2023

Artificial intelligence (AI)-based analysis has recently been adopted in the examination of histological slides via the digitization of glass slides using a digital scanner. In this study, we examined the effect of varying the staining color tone and magnification level of a dataset on the result of AI model prediction in hematoxylin and eosin stained whole slide images (WSIs). The WSIs of liver tissues with fibrosis were used as an example, and three different datasets (N20, B20, and B10) were prepared with different color tones and magnifications. Using these datasets, we built five models trained Mask R-CNN algorithm by a single or mixed dataset of N20, B20, and B10. We evaluated their model performance using the test dataset of three datasets. It was found that the models that were trained with mixed datasets (models B20/N20 and B10/B20), which consist of different color tones or magnifications, performed better than the single dataset trained models. Consequently, superior performance of the mixed models was obtained from the actual prediction results of the test images. We suggest that training the algorithm with various staining color tones and multi-scaled image datasets would be more optimized for consistent remarkable performance in predicting pathological lesions of interest.
Fractal dimension based geographical clustering of COVID-19 time series data
Authors: Natalia Yessika Adelwin , Faes Christel , Neyens Thomas , Chys Pieter , Hammami Naïma , Molenberghs Geert
Published: March 2023

Understanding the local dynamics of COVID-19 transmission calls for an approach that characterizes the incidence curve in a small geographical unit. Given that incidence curves exhibit considerable day-to-day variation, the fractal structure of the time series dynamics is investigated for the Flanders and Brussels Regions of Belgium. For each statistical sector, the smallest administrative geographical entity in Belgium, fractal dimensions of COVID-19 incidence rates, based on rolling time spans of 7, 14, and 21 days were estimated using four different estimators: box-count, Hall-Wood, variogram, and madogram. We found varying patterns of fractal dimensions across time and location. The fractal dimension is further summarized by its mean, variance, and autocorrelation over time. These summary statistics are then used to cluster regions with different incidence rate patterns using k-means clustering. Fractal dimension analysis of COVID-19 incidence thus offers important insight into the past, current, and arguably future evolution of an infectious disease outbreak.
Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy
Authors: Sorelli Michele , Costantini Irene , Bocchi Leonardo , Axer Markus , Pavone Francesco Saverio , Mazzamuto Giacomo
Published: March 2023

Fluorescence microscopy can be exploited for evaluating the brain’s fiber architecture with unsurpassed spatial resolution in combination with different tissue preparation and staining protocols. Differently from state-of-the-art polarimetry-based neuroimaging modalities, the quantification of fiber tract orientations from fluorescence microscopy volume images entails the application of specific image processing techniques, such as Fourier or structure tensor analysis. These, however, may lead to unreliable outcomes as they do not isolate myelinated fibers from the surrounding tissue. In this work, we describe a novel image processing pipeline that enables the computation of accurate 3D fiber orientation maps from both grey and white matter regions, exploiting the selective multiscale enhancement of tubular structures of varying diameters provided by a 3D implementation of the Frangi filter. The developed software tool can efficiently generate orientation distribution function maps at arbitrary spatial scales which may support the histological validation of modern diffusion-weighted magnetic resonance imaging tractography. Despite being tested here on two-photon scanning fluorescence microscopy images, acquired from tissue samples treated with a label-free technique enhancing the autofluorescence of myelinated fibers, the presented pipeline was developed to be employed on all types of 3D fluorescence images and fiber staining.
Exogenous melatonin induces phenolic compounds production in Linum album cells by altering nitric oxide and salicylic acid
Authors: Esmaeili Sara , Sharifi Mohsen , Ghanati Faezeh , Soltani Bahram M. , Samari Elaheh , Sagharyan Mostafa
Published: March 2023

Melatonin is a pleiotropic molecule that can influence various aspects of plant performance. Recent studies have exhibited that it mediates plant defensive responses, probably through managing redox homeostasis. We tried to track the regulatory effects of melatonin on the antioxidant machinery of Linum album cell culture. To this, different concentrations of melatonin were applied, and the oxidative status of cells was investigated by measuring the levels of oxidative molecules and antioxidant agents. The results showed that H2O2 content did not change at the low melatonin levels, while it increased at the high concentrations. It can be correlated with the low melatonin dosages capacity to remove excessive amounts of H2O2, while the high melatonin dosages exhibit toxicity effects. In contrast, the NO enhancement occurred at 50 μM melatonin, proposing its role in triggering melatonin-induced defensive responses. The MDA results stated that NO led to oxidative stress in melatonin-treated cells at 50 μM melatonin. Antioxidant enzyme POD was activated by melatonin treatment, while SOD enzyme behaved reversely which can explain the changes in the H2O2 level. In addition, the analysis of the phenolics profile showed that the contents of phenolic acids, flavonoids, and lignans enhanced following an increase in PAL enzyme activity. The increased level of phenolic hormone SA can indicate that melatonin affects the defensive responses in L. album cells through a SA-dependent pathway. In general, it seems that melatonin, by modulating NO and SA levels, can induce the activity of antioxidant enzymes and the production of phenolics, especially lignans, in L. album cells.
Diel variations in planktonic ciliate community structure in the northern South China Sea and tropical Western Pacific
Authors: Wang Chaofeng , Dong Yi , Denis Michel , Wei Yuanyuan , Li Haibo , Zheng Shan , Zhang Wuchang , Xiao Tian
Published: March 2023

Though diel variations are geographically widespread phenomena among phytoplankton and zooplankton, knowledge is limited regarding diel variations in planktonic ciliate (microzooplankton) community structure. In this study, we analyzed diel variations in community structure of planktonic ciliates in the northern South China Sea (nSCS) and tropical Western Pacific (tWP). Hydrological characteristics during day and night were slightly different over both the nSCS and tWP, while ciliate average abundance at night was clearly higher than in the day in the upper 200 m. In both the nSCS and tWP, abundance proportions of large size-fraction (> 30 μm) aloricate ciliates at night were higher than in the day. While for tintinnids, abundance proportion of large lorica oral diameter at night were lower than in the day. The relationship between environmental factors and ciliate abundance pointed out that depth and temperature were main factors influencing aloricate ciliate and tintinnid in both day and night. For some dominant tintinnid species, chlorophyllawas another important factor influencing their diel vertical distribution. Our results provide fundamental data for better understanding the mechanisms of planktonic ciliate community diel variation in the tropical Western Pacific Ocean.
Analysis of available animal testing data to propose peer-derived quantitative thresholds for determining adequate surveillance capacity for rabies
Authors: Minhaj Faisal S. , Bonaparte Sarah C. , Boutelle Cassandra , Wallace Ryan M.
Published: March 2023

Historical targets for country-level animal rabies testing volumes were abandoned due to ethical and welfare concerns, and interpretation challenges of testing healthy animals. To-date, no quantitative threshold has been established for evaluating adequate surveillance capacity specific to suspected rabid animals. The purpose here is to establish quantitative testing thresholds for rabies suspected animals to assess a country’s rabies surveillance capacity. Animal rabies testing data was obtained from official and unofficial rabies surveillance platforms from 2010 to 2019 and supplemented with official country reports and published literature. Testing rates were determined for all-animal and domestic animals, and standardized per 100,000 estimated human population; the domestic animal rate was also standardized per 100,000 estimated dog population. There were 113 countries that reported surveillance data eligible for analysis. Countries reporting the most data were under WHO categories as having endemic human rabies or no dog rabies. The annual median all-animal testing rate for all countries was 1.53 animals/100,000 human population (IQR 0.27–8.78). Three proposed testing rate thresholds are an all-animal rate of 1.9 animals/100,000 humans, a domestic animal per human rate of 0.8 animals/100,000 humans, and a domestic animal per dog rate of 6.6 animals/100,000 dogs. These three peer-derived rabies testing thresholds for passive surveillance can be used to facilitate assessment of a country’s rabies surveillance capacity.