bluuebunny/crossref_metadata_2025 huggingface dataset:
The Macrotextured Implant Recall: Breast Implant–Associated-Anaplastic Large Cell Lymphoma Risk Aversion in Cosmetic and Reconstructive Plastic Surgery Practices
Authors: Mankowski Peter , Carr Matthew , Cherukupalli Abhiram , Bovill Esta , Lennox Peter , Brown Mitchell H , Carr Nicholas
Published: June 2022

Background

The recall of Allergan Biocell (Irvine, CA) devices due to the association between anaplastic large cell lymphoma (ALCL) and macrotextured breast implants means that plastic surgeons are faced with the challenge of caring for patients with these implants in situ. Cosmetic and reconstructive surgeons have been contacting affected patients to encourage them to follow up and discuss the most appropriate risk-reduction strategies.

Objectives

The aim of this study was to evaluate patient concerns about the risk of breast implant–associated ALCL (BIA-ALCL) and to compare management differences between cosmetic and reconstructive patients.

Methods

A retrospective review was performed of 432 patients with macrotextured implants who presented to clinic after being contacted (121 reconstructive and 311 cosmetic). These records were analyzed for their presenting concerns, surgery wait times, and management plans. Statistical analysis was performed to compare the cohorts, and odds ratios (ORs) were computed to determine the association between patient concerns and their choice of management.

Results

After consultation, 59.5% of the reconstructive cohort and 49.5% of the cosmetic cohort scheduled implant removal or exchange. The reconstructive population had a higher rate of ALCL concern (62.7%); however, both cohorts had a significant OR, demonstrating an expressed fear of ALCL likely contributed to their subsequent clinical management (OR cosmetic, 1.66; OR reconstructive, 2.17).

Conclusions

Although the risk of ALCL appears to be more concerning to the reconstructive population, both cohorts were equally motivated to have their implants removed. Informing patients about their ALCL risk is crucial to ensure a patient-supported risk reduction plan.

Level of Evidence: 2
Dissociating the functional roles of arcuate fasciculus subtracts in speech production
Authors: Janssen Nikki , Kessels Roy P C , Mars Rogier B , Llera Alberto , Beckmann Christian F , Roelofs Ardi
Published: June 2022

Recent tractography and microdissection studies have shown that the left arcuate fasciculus (AF)—a fiber tract thought to be crucial for speech production—consists of a minimum of 2 subtracts directly connecting the temporal and frontal cortex. These subtracts link the posterior superior temporal gyrus (STG) and middle temporal gyrus (MTG) to the inferior frontal gyrus. Although they have been hypothesized to mediate different functions in speech production, direct evidence for this hypothesis is lacking. To functionally segregate the 2 AF segments, we combined functional magnetic resonance imaging with diffusion-weighted imaging and probabilistic tractography using 2 prototypical speech production tasks, namely spoken pseudoword repetition (tapping sublexical phonological mapping) and verb generation (tapping lexical-semantic mapping). We observed that the repetition of spoken pseudowords is mediated by the subtract of STG, while generating an appropriate verb to a spoken noun is mediated by the subtract of MTG. Our findings provide strong evidence for a functional dissociation between the AF subtracts, namely a sublexical phonological mapping by the STG subtract and a lexical-semantic mapping by the MTG subtract. Our results contribute to the unraveling of a century-old controversy concerning the functional role in speech production of a major fiber tract involved in language.
Preferential uptake of SARS-CoV-2 by pericytes potentiates vascular damage and permeability in an organoid model of the microvasculature
Authors: Khan Abdullah O , Reyat Jasmeet S , Hill Harriet , Bourne Joshua H , Colicchia Martina , Newby Maddy L , Allen Joel D , Crispin Max , Youd Esther , Murray Paul G , Taylor Graham , Stamataki Zania , Richter Alex G , Cunningham Adam F , Pugh Matthew , Rayes Julie
Published: June 2022

Aims

Thrombotic complications and vasculopathy have been extensively associated with severe COVID-19 infection; however, the mechanisms inducing endotheliitis and the disruption of endothelial integrity in the microcirculation are poorly understood. We hypothesized that within the vessel wall, pericytes preferentially take up viral particles and mediate the subsequent loss of vascular integrity.

Methods and results

Immunofluorescence of post-mortem patient sections was used to assess pathophysiological aspects of COVID-19 infection. The effects of COVID-19 on the microvasculature were assessed using a vascular organoid model exposed to live viral particles or recombinant viral antigens. We find increased expression of the viral entry receptor angiotensin-converting enzyme 2 on pericytes when compared to vascular endothelium and a reduction in the expression of the junctional protein CD144, as well as increased cell death, upon treatment with both live virus and/or viral antigens. We observe a dysregulation of genes implicated in vascular permeability, including Notch receptor 3, angiopoietin-2, and TEK. Activation of vascular organoids with interleukin-1β did not have an additive effect on vascular permeability. Spike antigen was detected in some patients’ lung pericytes, which was associated with a decrease in CD144 expression and increased platelet recruitment and von Willebrand factor (VWF) deposition in the capillaries of these patients, with thrombi in large vessels rich in VWF and fibrin.

Conclusion

Together, our data indicate that direct viral exposure to the microvasculature modelled by organoid infection and viral antigen treatment results in pericyte infection, detachment, damage, and cell death, disrupting pericyte-endothelial cell crosstalk and increasing microvascular endothelial permeability, which can promote thrombotic and bleeding complications in the microcirculation.
Safety of Ustekinumab and Vedolizumab During Pregnancy—Pregnancy, Neonatal, and Infant Outcome: A Prospective Multicentre Study
Authors: Mitrova Katarina , Pipek Barbora , Bortlik Martin , Bouchner Ludek , Brezina Jan , Douda Tomas , Drasar Tomas , Klvana Pavel , Kohout Pavel , Leksa Vaclav , Minarikova Petra , Novotny Ales , Svoboda Pavel , Skorpik Jan , Ulbrych Jan , Veinfurt Marek , Zborilova Blanka , Lukas Milan , Duricova Dana ,   Czech IBD Working Group
Published: June 2022

Background and Aims

Evidence on the safety of newer biologics during pregnancy is limited. We aimed to assess the safety of ustekinumab and vedolizumab treatment during gestation on pregnancy and infant outcome. Furthermore, we evaluated the placental transfer of these agents.

Methods

We performed a prospective, multicentre, observational study in consecutive women with inflammatory bowel disease exposed to ustekinumab or vedolizumab 2 months prior to conception or during pregnancy. Pregnancy, neonatal, and infant outcomes were evaluated and compared with the anti-tumour necrosis factor [TNF]-exposed control group. Drug levels were assessed in maternal and cord blood at delivery.

Results

We included 54 and 39 pregnancies exposed to ustekinumab and vedolizumab, respectively. In the ustekinumab group, 43 [79.9%] resulted in live births, and 11 [20.4%] led to spontaneous abortion. Thirty-five [89.7%] pregnancies on vedolizumab ended in a live birth, two [5.1%] in spontaneous, and two [5.1%] in therapeutic abortion. No significant difference in pregnancy outcome between either the vedolizumab or the ustekinumab group and controls was observed [p >0.05]. Similarly, there was no negative safety signal in the postnatal outcome of exposed children regarding growth, psychomotor development, and risk of allergy/atopy or infectious complications. The median infant-to-maternal ratio of ustekinumab levels was 1.67 and it was 0.59 in vedolizumab.

Conclusions

Use of ustekinumab and vedolizumab in pregnancy seems to be safe, with favuorable pregnancy and postnatal infant outcomes. Placental transfer differed between these two drugs, with ustekinumab having similar and vedolizumab having inverse infant-to-maternal ratio of drug levels compared with anti-TNF preparations.
Delivering the Undeliverable: Teaching English in a University Today
Authors: Moran Joe
Published: June 2022

In UK universities, English has seen a steep fall in admissions, in the context of a general decline in humanities enrolments and changes to the school curriculum which have turned students away from the subject. This article explores what teaching English in a university means in these difficult times. It asks: what actually happens in an English class? What do students learn? And why is it so hard to make the case for English, to find a form of words in today’s educational vocabulary that will convince sceptics of its value? The subject’s declining status is part of a more general sense that technological change and the free market have answered all the key questions about what skills we need to learn and how we should live our lives. We frame the delivery of teaching in terms suggested by technology and the market – as a frictionless process with a predictable and measurable outcome. This does not sit well with the untidily human, stochastic, accretive nature of humanities teaching. And yet the skills of close reading cultivated in literary study can teach students to plot a course through contemporary life. They can help them to navigate a digitized, online, data-driven world whose meanings and values are filtered through unexamined words and unacknowledged stories.
Where DESO Disappears: Spatial Inequality and Social Stratification at Labour Market Entry
Authors: Morris Katy
Published: June 2022

At country-level, a host of evidence suggests there is a sizeable direct effect of social origin (DESO) on initial labour market outcomes, net of educational attainment. What is true at country-level is not always true below country-level, however. Using data from the UK Household Longitudinal Survey and the German Socio-Economic Panel, we show that variable spatial opportunity structures moderate the size of DESO at labour market entry, such that there are places where DESO disappears. Social origins assume greater importance as local labour market conditions deteriorate: in weak local labour markets, non-graduates are approximately 16 percentage points less likely to find employment if their parents are care workers rather than secondary school teachers, while graduates typically obtain first jobs that are 7–9 ISEI points lower in status. These findings highlight the distinctive geography of social stratification processes at labour market entry and potentially beyond.
Psychological distress and quality of life in asymptomatic adults following provision of imaging results for prevention of cardiovascular disease events: a scoping review
Authors: Anokye Reindolf , Jackson Ben , Dimmock James , Dickson Joanne M , Blekkenhorst Lauren C , Hodgson Jonathan M , Lewis Joshua R , Stanley Mandy
Published: June 2022

Aims

Psychological distress and changes in health-related quality of life (HRQoL) may occur after screening for disease. Reporting outcomes related to potential benefits and harms of screening is a key recommendation in the guidelines for reporting high-quality trials or interventions. However, no reviews have directly investigated outcomes related to psychological distress and/or changes in HRQoL following imaging assessment of cardiovascular risk and communication of cardiovascular findings to asymptomatic adults. A scoping review was conducted to map research on psychological distress and/or HRQoL following screening.

Methods and results

Six electronic databases (MEDLINE, PsychINFO, Social Work Abstracts, Psychology and Behavioural Sciences Collection, CINAHL, and EMBASE) were searched for articles that assessed psychological distress and/or HRQoL following screening. Two investigators independently screened titles and abstracts for all records retrieved using predefined criteria. Studies were conducted among active smokers, military personnel, athletes, post-menopausal women, and high-risk individuals. Seven constructs related to psychological distress and HRQoL appeared across 11 articles (randomized controlled trials, n = 4 and non-randomized studies, n = 7). Worry, depression, perceived stress, anxiety, and quality of life were most prominent. Multiple-item measures of psychological distress (e.g. Taylor Anxiety Score and Beck Depression Inventory) were used in 5/9 (56%) studies. Key findings on psychological distress and/or changes in HRQoL following screening were mixed.

Conclusions

Findings support the need for multiple-item measures with better psychometric properties to examine the psychological responses to screening results in future studies. Strategies to support individuals during and following vascular screening to maximise potential benefits of screening and minimize harms are discussed.
Understanding and assessing gamification in digital healthcare interventions for patients with cardiovascular disease
Authors: Berglund Aseel , Jaarsma Tiny , Berglund Erik , Strömberg Anna , Klompstra Leonie
Published: June 2022

Gamification is defined as the use of game design elements in contexts other than gaming to increase user engagement and experience. Gamification in cardiovascular care can contribute to positively change health behaviour with possible effects and benefits on physical health and mental well-being. Based on previous literature, in this article we describe: the conceptualization of gamification, the five gamification principles for gamified digital health programmes or applications, the six most common game elements used to impact health behaviour applied in gamified digital health interventions and finally scientifically validated instruments to use for assessment of gamification in terms of self-reported psychological outcomes.
Examining how congruence in and satisfaction with dyadic care type appraisal contribute to quality of life in heart failure care dyads
Authors: Irani Elliane , Buck Harleah G , Lyons Karen S , Margevicius Seunghee , Vellone Ercole , Bugajski Andrew , De Maria Maddalena
Published: June 2022

Aims

Given the complexity of heart failure (HF) management, persons with HF and their informal caregivers often engage in dyadic illness management. It is unknown how congruent appraisal of dyadic HF care type is associated with dyadic health. Our aim was to examine how congruence in and satisfaction with appraisal of dyadic HF care type contribute to quality of life (QOL) for dyads.

Methods and results

This is a secondary analysis of cross-sectional data on 275 HF care dyads (patients 45.1% female, caregivers 70.5% female). Congruent appraisal and satisfaction were assessed using the Dyadic Symptom Management Type instrument. Quality of life was measured using the Short Form-12. Multilevel dyadic models were estimated to examine the contribution of congruence and satisfaction with dyadic care type to physical and mental QOL. Congruent appraisal of dyadic care type was positively associated with caregivers’ mental QOL (B = 2.69, P = 0.026). Satisfaction with dyadic care type was positively associated with physical and mental QOL for persons with HF (B = 1.58, P = 0.011 and B = 2.09, P = 0.002, respectively) and informal caregivers (B = 1.70, P = 0.004 and B = 2.90, P < 0.001, respectively), while controlling for age, New York Heart Association class, daily hours spent together, relationship type, and congruence with dyadic care type.

Conclusion

Satisfaction with dyadic care type appraisal was a stronger contributor to QOL for HF care dyads, compared with congruent appraisals. It is important to understand reasons for dissatisfaction within the dyad to assist dyad members in reaching shared appraisals while managing HF.
Exercise oscillatory breathing in heart failure with reduced ejection fraction: clinical implication
Authors: da Luz Goulart Cássia , Agostoni Piergiuseppe , Salvioni Elisabetta , Kaminsky Leonard A , Myers Jonathan , Arena Ross , Borghi-Silva Audrey
Published: June 2022

Aim

The aim of the study is (i) to evaluate the impact of exercise oscillatory ventilation (EOV) in patients with heart failure (HF) with reduced left ventricular ejection fraction (HFrEF) during cardiopulmonary exercise testing (CPET) compared with no EOV (N-EOV); (ii) to identify the influence of EOV persistence (P-EOV) and EOV disappearance (D-EOV) during CPET on the outcomes of mortality and hospitalization in HFrEF patients; and (iii) to identify further predictors of mortality and hospitalization in patients with P-EOV.

Methods and results

Three hundred and fifteen stable HFrEF patients underwent CPET and were followed for 35 months. We identified 202 patients N-EOV and 113 patients with EOV. Patients with EOV presented more symptoms [New York Heart Association (NYHA) III: 35% vs. N-EOV 20%, P < 0.05], worse cardiac function (LVEF: 28 ± 6 vs. N-EOV 39 ± 1, P < 0.05), higher minute ventilation/carbon dioxide production (V̇E/V̇CO2 slope: 41 ± 11 vs. N-EOV 37 ± 8, P < 0.05) and a higher rate of deaths (26% vs. N-EOV 6%, P < 0.05) and hospitalization (29% vs. N-EOV 9%, P < 0.05). Patients with P-EOV had more severe HFrEF (NYHA IV: 23% vs. D-EOV: 9%, P < 0.05), had worse cardiac function (LVEF: 24 ± 5 vs. D-EOV: 34 ± 3, P < 0.05) and had lower peak oxygen consumption (V̇O2) (12.0 ± 3.0 vs. D-EOV: 13.3 ± 3.0 mLO2 kg–1.min–1, P < 0.05). Among P-EOV, other independent predictors of mortality were V̇E/V̇CO2 slope ≥36 and V̇O2 peak ≤12 mLO2 kg–1 min–1; a V̇E/V̇CO2 slope≥34 was a significant predictor of hospitalization. Kaplan–Meier survival analysis showed that HFrEF patients with P-EOV had a higher risk of mortality and higher risk of hospitalization (P < 0.05) than patients with D-EOV and N-EOV.

Conclusion

In HFrEF patients, EOV persistence during exercise had a strong prognostic role. In P-EOV patients, V̇E/V̇CO2 ≥36 and V̇O2 peak ≤12 mLO2 kg–1 min–1 had a further additive negative prognostic role.
Histamine H2 receptor antagonist exposure was related to decreased all-cause mortality in critical ill patients with heart failure: a cohort study
Authors: Huang Yan-Hua , Cai Wen-ke , Yin Sun-Jun , Wang Ping , Li Zhi-Ran , Yang Qin , Zhou Tao , Meng Rui , Yang Mei , Guo Yu , He Gong-Hao
Published: June 2022

Aims

Previous studies reported that histamine H2 receptor antagonists (H2RAs) had cardioprotective effects. However, the effect of H2RAs on mortality of critical ill patients with heart failure (HF) remains unclear. The aim of this study was to clarify the association between H2RAs and all-cause mortality of critical ill patients with HF based on Medical Information Mart for Intensive Care III database (MIMIC-III).

Methods and results

Propensity score matching (PSM) was applied to account for the baseline differences between two groups that were exposed to H2RAs or not. The study primary outcome was all-cause mortality. Kaplan–Meier curves and multivariable Cox regression models were employed to estimate the effects of H2RAs on mortality of critical ill patients with HF. A total of 10 387 patients were included, involving 4440 H2RAs users and 5947 non-H2RAs users. After matching, 3130 pairs of patients were matched between H2RAs users and non-H2RAs users. The results showed significant association between H2RAs exposure and decreased 30-day, 90-day, and 1-year mortality in both univariate analyses and multivariate analyses [hazard ratio (HR) = 0.73, 95% confidence interval (CI): 0.65–0.83 for 30-day; HR = 0.80, 95%CI: 0.72–0.89 for 90-day; and HR = 0.83, 95%CI: 0.76–0.90 for 1-year mortality, respectively] by Cox regression after PSM. Furthermore, stratified analyses revealed that the 30-day, 90-day, and 1-year mortality of ranitidine users were significantly lower than those of famotidine users, respectively.

Conclusion

Histamine H2 receptor antagonists exposure was associated with lower mortality in critical ill patients with HF. Furthermore, ranitidine might be superior to famotidine in reducing mortality of critical ill patients with HF.
Isolation, diversity and antimicrobial activity of planctomycetes from the Tejo river estuary (Portugal)
Authors: Vitorino Inês Rosado , Lobo-da-Cunha Alexandre , Vasconcelos Vítor , Vicente Francisca , Lage Olga Maria
Published: June 2022

The discovery of new bioactive compounds is an invaluable aid to the development of new drugs. Strategies for finding novel molecules can focus on the exploitation of less studied organisms and ecosystems such as planctomycetes and brackish habitats. The unique cell biology of the underexplored Planctomycetota mean it is of particular interest. In this study, we aimed to isolate planctomycetes from the estuary of the Tejo river (Portugal). To reach this goal, macroalgae, water and sediments were sampled and diverse media and isolation techniques applied. Sixty-nine planctomycetal strains were brought into pure culture. An analysis of the 16S rRNA genes found that the majority of the isolates were affiliated to the genus Rhodopirellula. Putative novel taxa belonging to genera Stieleria and Rhodopirellula were also isolated and characterized morphologically. Enterobacterial repetitive intergenic consensus fingerprinting analyses showed higher diversity and different genotypes within close strains. Relevant biosynthetic gene clusters were found in most isolates and acetone extracts from representative strains exhibited mild antimicrobial activities against Escherichia coli and Staphylococcus aureus. Our work has not only enlarged the number and diversity of cultured planctomycetes but has also shown the potential for the discovery of bioactive compounds from the novel taxa.
The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit
Authors: Bean Björn D M , Whiteway Malcolm , Martin Vincent J J
Published: June 2022

The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) - CRISPR-associated protein 9 (Cas9)-based genome editing tools, we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 toolkit with 3 components: (1) a set of optimized Streptococcus pyogenes Cas9-guide RNA expression vectors with 5 selectable markers and the option to either preclone or cotransform the gRNAs; (2) vectors for the one-step construction of integration cassettes expressing an untagged or green fluorescent protein/red fluorescent protein/hemagglutinin-tagged gene of interest at one of 3 levels, supporting localization and overexpression studies; and (3) integration cassettes containing moderately expressed green fluorescent protein- or red fluorescent protein-tagged compartmental markers for colocalization experiments. These components allow rapid, high-efficiency genomic integrations and modifications with only transient selection for the Cas9 vector, resulting in markerless transformations. To demonstrate the ease of use, we applied our complete set of compartmental markers to colabel all target subcellular compartments with green fluorescent protein and red fluorescent protein. Thus, the MyLO toolkit packages CRISPR-Cas9 technology into a flexible, optimized bundle that allows the stable genomic integration of DNA with the ease of use approaching that of transforming plasmids.
The association between disruption of the circadian rhythm and aggravation of colitis in mice
Authors: Chen Yi-Dong , Zhao Rui-Feng , Zheng Gen , Ling Fang-Mei , Li Jun-Rong , Xu Ming-Yang , Guo Di , Zhang Qiu-Lei , Li Shuang , Zhu Liang-Ru
Published: June 2022

Delayed recovery from ulcerative colitis is mainly due to impaired healing of the intestinal epithelium after inflammation. The circadian rhythm controls cell proliferation and energy metabolism. However, the role of circadian genes in inflammatory bowel disease is largely unknown. The purpose of this study was to investigate whether disrupting the circadian rhythm in mice can worsen colitis by altering mitochondrial energy metabolism. Mice in the experimental groups were under physiologic stress with an 8-h light shift jet-lag schedule every 3 days, whereas those in the control group were not. Subsequently, half of the mice in the control and jet-lagged groups were given dextran sodium sulfate (DSS) to induce colitis. Mice in each group were euthanized at zeitgeber time (ZT)0, ZT4, ZT8, ZT12, ZT16, and ZT20. To investigate the effects of jet lag on the mice, colon specimens were subjected to hematoxylin and eosin staining to analyse mRNA and protein expression of core circadian clock genes (Bmal1, Clock, Per1, Per2, Cry1, Cry2, and Nr1d1). We analysed the mitochondrial morphology, adenosine triphosphate (ATP) levels, and the expression of dynamin-related protein 1 (Drp1) and ser637-phosphorylated (p)-Drp1, which are closely related to ATP production. We further investigated the effect of PER2 knock-down in the colon epithelial cells (CCD 841 CoN) by measuring ATP and cell proliferation levels. Disrupting the circadian rhythm changed the oscillation of clock genes in the colon of mice, altered the mitochondrial morphology of the colon specimens, decreased the expression of p-Drp1, reduced ATP production, and exacerbated inflammatory responses in mice with DSS-induced colitis. Additionally, silencing of PER2 in the colon epithelial cells reduced ATP production and cell proliferation. Disrupting the circadian rhythm in mice decreases mitochondrial energy metabolism in the colon and exacerbates symptoms of colitis.
A 3-D crustal shear wave velocity model and Moho map below the Semail Ophiolite, eastern Arabia
Authors: Weidle C , Wiesenberg L , El-Sharkawy A , Krüger F , Scharf A , Agard P , Meier T
Published: June 2022

The Semail Ophiolite in eastern Arabia is the largest and best-exposed slice of oceanic lithosphere on land. Detailed knowledge of the tectonic evolution of the shallow crust, in particular during and after ophiolite obduction in Late Cretaceous times is contrasted by few constraints on physical and compositional properties of the middle and lower continental crust below the obducted units. The role of inherited, pre-obduction crustal architecture remains therefore unaccounted for in our understanding of crustal evolution and the present-day geology. Based on seismological data acquired during a 27-month campaign in northern Oman, Ambient Seismic Noise Tomography and Receiver Function analysis provide for the first time a 3-D radially anisotropic shear wave velocity (VS) model and a consistent Moho map below the iconic Semail Ophiolite. The model highlights deep crustal boundaries that segment the eastern Arabian basement in two distinct units. The previously undescribed Western Jabal Akhdar Zone separates Arabian crust with typical continental properties and a thickness of ∼40–45 km in the northwest from a compositionally different terrane in the southeast that is interpreted as a terrane accreted during the Pan-African orogeny in Neoproterozoic times. East of the Ibra Zone, another deep crustal boundary, crustal thickness decreases to 30–35 km and very high lower crustal VS suggest large-scale mafic intrusions into, and possible underplating of the Arabian continental crust that occurred most likely during Permian breakup of Pangea. Mafic reworking is sharply bounded by the (upper crustal) Semail Gap Fault Zone, northwest of which no such high velocities are found in the crust. Topography of the Oman Mountains is supported by a mild crustal root and Moho depth below the highest topography, the Jabal Akhdar Dome, is ∼42 km. Radial anisotropy is robustly resolved in the upper crust and aids in discriminating dipping allochthonous units from autochthonous sedimentary rocks that are indistinguishable by isotropic VS alone. Lateral thickness variations of the ophiolite highlight the Haylayn Ophiolite Massif on the northern flank of Jabal Akhdar Dome and the Hawasina Window as the deepest reaching unit. Ophiolite thickness is ∼10 km in the southern and northern massifs, and ≤5 km elsewhere.
Carnosol, a diterpene present in rosemary, increases ELP1 levels in familial dysautonomia patient-derived cells and healthy adults: a possible therapy for FD
Authors: Anderson Sylvia L , Fasih-Ahmad Faaria , Evans Anthony J , Rubin Berish Y
Published: June 2022

Recent research on familial dysautonomia (FD) has focused on the development of therapeutics that facilitate the production of the correctly spliced, exon 20-containing, transcript in cells and individuals bearing the splice-altering, FD-causing mutation in the elongator acetyltransferase complex subunit I (ELP1) gene. We report here the ability of carnosol, a diterpene present in plant species of the Lamiaceae family, including rosemary, to enhance the cellular presence of the correctly spliced ELP1 transcript in FD patient-derived fibroblasts by upregulating transcription of the ELP1 gene and correcting the aberrant splicing of the ELP1 transcript. Carnosol treatment also elevates the level of the RNA binding motif protein 24 (RBM24) and RNA binding motif protein 38 (RBM38) proteins, two multifunctional RNA-binding proteins. Transfection-mediated expression of either of these RNA binding motif (RBMs) facilitates the inclusion of exon 20 sequence into the transcript generated from a minigene-bearing ELP1 genomic sequence containing the FD-causing mutation. Suppression of the carnosol-mediated induction of either of these RBMs, using targeting siRNAs, limited the carnosol-mediated inclusion of the ELP1 exon 20 sequence. Carnosol treatment of FD patient peripheral blood mononuclear cells facilitates the inclusion of exon 20 into the ELP1 transcript. The increased levels of the ELP1 and RBM38 transcripts and the alternative splicing of the sirtuin 2 (SIRT2) transcript, a sentinel for exon 20 inclusion in the FD-derived ELP1 transcript, are observed in RNA isolated from whole blood of healthy adults following the ingestion of carnosol-containing rosemary extract. These findings and the excellent safety profile of rosemary together justify an expedited clinical study of the impact of carnosol on the FD patient population.
Substance Use in Relation to Mental Illness: A Study of Mexican-Origin Latinos with Schizophrenia and Their Family Members
Authors: Hernandez Mercedes , Barrio Concepcion , Helu-Brown Paula , Lim Caroline , Gaona Lizbeth
Published: June 2022

Little is known about family context and substance use behaviors among Latinos with schizophrenia. Learning about patient and family caregiver perceptions of use is critical to our understanding of how best to support these vulnerable patients and family caregivers. This study explored perceptions of substance use in relation to mental illness among Latinos primarily of Mexican origin with schizophrenia and their family caregivers. Semistructured interviews were conducted with 34 participants (20 family caregivers; 14 patients) with direct and indirect substance use experience, in their preferred language. Data were analyzed using thematic analysis, which consisted of comparing codes across and within patient and family caregiver transcripts. Findings revealed that substance use affected well-being, particularly patient recovery and caregiver burden. Strategies to address well-being included limiting substance use, being vigilant about patient use, and communicating the negative impact of use. The environmental impact and stigma of substance use were major contextual challenges. Patients addressed these by limiting their socialization. Family social context was important to how substance use was perceived and managed by patients and caregivers. As such, treatment models should consider a holistic perspective that incorporates family context when addressing substance use among Latinos with schizophrenia.
Automatic information extraction from childhood cancer pathology reports
Authors: Yoon Hong-Jun , Peluso Alina , Durbin Eric B , Wu Xiao-Cheng , Stroup Antoinette , Doherty Jennifer , Schwartz Stephen , Wiggins Charles , Coyle Linda , Penberthy Lynne
Published: June 2022

Objectives

The International Classification of Childhood Cancer (ICCC) facilitates the effective classification of a heterogeneous group of cancers in the important pediatric population. However, there has been no development of machine learning models for the ICCC classification. We developed deep learning-based information extraction models from cancer pathology reports based on the ICD-O-3 coding standard. In this article, we describe extending the models to perform ICCC classification.

Materials and Methods

We developed 2 models, ICD-O-3 classification and ICCC recoding (Model 1) and direct ICCC classification (Model 2), and 4 scenarios subject to the training sample size. We evaluated these models with a corpus consisting of 29 206 reports with age at diagnosis between 0 and 19 from 6 state cancer registries.

Results

Our findings suggest that the direct ICCC classification (Model 2) is substantially better than reusing the ICD-O-3 classification model (Model 1). Applying the uncertainty quantification mechanism to assess the confidence of the algorithm in assigning a code demonstrated that the model achieved a micro-F1 score of 0.987 while abstaining (not sufficiently confident to assign a code) on only 14.8% of ambiguous pathology reports.

Conclusions

Our experimental results suggest that the machine learning-based automatic information extraction from childhood cancer pathology reports in the ICCC is a reliable means of supplementing human annotators at state cancer registries by reading and abstracting the majority of the childhood cancer pathology reports accurately and reliably.
Improvement in the Reliability of AOAC Official MethodSM 2012.15 for Iodine, Part-2
Authors: Hieda Naoto , Nishida Motoyuki , Matsuno Ichirou
Published: June 2022

Background

In our previous study, the AOAC Official MethodSM2012.15 (Method 2012.15) was modified by adding carbon (as methanol) to the standard and final test solutions for accurate quantification of iodine in infant and special formulas after incomplete matrix oven digestion. However, when an inductively coupled plasma-mass spectrometry (ICP-MS) instrument from a different manufacturer was used, inaccurately low iodine content values were obtained using both the original and modified methods.

Objective

To obtain more reliable values for infant formulas using varying ICP-MS instruments.

Methods

The protocol was modified by changing the ratio of the pump tubing inner diameters (IDs) for the ICP-MS system or by optimizing the internal standards for Method 2012.15.

Results

No significant improvement was observed by changing the ratio of the pump tubing IDs. In the optimization study of the internal standard, when germanium is used as the internal standard, the measurement result of NIST SRM® 1849 was within the certified value range. Furthermore, with the addition of methanol equivalent to 5% to the standard and final test solution based on our previous study, the recovery of the samples was within the certified range of SMPR 2012.008.

Conclusions

This demonstrates that changing the internal standard to germanium and adding methanol equivalent to 5％ to the standard and final test solution, can result in stable measurement even when the ICP-MS specifications were changed.

Highlights

The modified Method 2012.15 provides reliable results even if the instrument is changed by changing the internal standard to germanium.
Adherence to Burn Resuscitation Guidelines Reduces Resuscitation Fluids and Mortality
Authors: Mankowski Peter , Papp Bettina , Genoway Krista , Papp Anthony
Published: June 2022

In our province, regional recommendations for optimal fluid resuscitation were published in 2011 to improve the management of acute burn patients prior to transfer to a specialized burn center. The purpose of this study was to determine compliance with these provincial burn resuscitation guidelines and their subsequent impact on patient outcomes. A retrospective review of patients transferred to the provincial burn center after being initially managed at peripheral sites was performed from 2011 to 2019. Patients were included if their burn injury was greater than 20% TBSA and they were transferred within 24 hours postburn injury. Charts were reviewed for the amount of fluid patients received and resuscitation associated outcomes. A total of 72 patients met the inclusion criteria, 37 of which were treated in accordance with the 2011 guidelines. For patients that followed the 2011 provincial guidelines, they received on average 3.2 cc/kg/TBSA of fluid during the first 24 hours postburn injury. Significantly more fluids were given when guidelines were not followed with an average of 4.4 cc/kg/TBSA (P = .03). Mortality rates were found to be significantly lower during the primary admission with guidelines compliance (16.2% vs 2.7%, P = .04). No significant differences were found between the remaining evaluated complications including abdominal compartment syndrome (8.1% vs 2.7%) and need for escharotomy (35.2% vs 21.6%). The use of clinical practice guidelines decreased excess IV fluid administration in additional to decreasing mortality rates for patients initially assessed in peripheral low volume centers.
When Products Come Alive: Interpersonal Communication Norms Induce Positive Word of Mouth for Anthropomorphized Products
Authors: Chen Fangyuan , Sengupta Jaideep , Zheng Jianqing (Frank)
Published: June 2022

Across five experiments, this research finds that product anthropomorphism enhances consumers’ intention to share positive thoughts in their word-of-mouth (WOM) communication about such products, in the hope of creating a favorable interpersonal impression about themselves. Our theorizing suggests that the effect occurs because consumers apply a norm that originates in human-related communication—namely, that speaking positively of other people creates a more likable impression of speakers by making them seem more kind and polite—to their WOM for anthropomorphized products (study 1). As a result, when an impression management motive is salient, consumers display greater overall positivity in their WOM for an anthropomorphized product than for its non-anthropomorphized equivalent (study 2). Support for this prediction is found across various measures of WOM positivity. Furthermore, in line with this conceptualization, anthropomorphism-induced positivity diminishes (a) when consumers are less concerned about impression management, such as when talking to a close friend (study 3), (b) when an opposing accuracy motive overpowers the impression management motive (study 4), or (c) when the underlying belief that positivity will yield favorable impressions is itself challenged (study 5). Our conceptualization and findings inform and extend research on consumer WOM communication, product anthropomorphism, and impression management.
Super-resolution reconstruction based on BM3D and compressed sensing
Authors: Cheng Tao , Jia Dongdong
Published: June 2022

In the various papers published in the field of super-resolution microscopy, denoising of raw images based on block-matching and 3D filtering (BM3D) was rarely reported. BM3D for blocks of different sizes was studied. The denoising ability is related to block sizes. The larger the block is, the better the denoising effect is. When the block size is >40, a good denoising effect can be achieved. Denoising has a great influence on the super-resolution reconstruction effect and the reconstruction time. Better super-resolution reconstruction and shorter reconstruction time can be achieved after denoising. Using compressed sensing, only 20 raw images are needed for super-resolution reconstruction. The temporal resolution is less than half a second. The spatial resolution is also greatly improved.
A case series of bread clip ingestions
Authors: Chuang Fred J , Townend Philip J , Cooper Michelle L
Published: June 2022

The bread clip is one of the most insidious foreign body ingested. The bread clip poses a serious medical danger to patients yet may often fail to manifest itself clinically on initial ingestion. We present a case series of three patients with bread clips ingestions that were managed in the Gold Coast University Hospital, Queensland, Australia between 2020 and 2021. Bread clips are not always readily identifiable depending on imaging and the management of these patients will often require a multidisciplinary approach between the surgeons, gastroenterologists and radiologists.
Urinary retention unveiling deeply embedded multiple leiomyomas in women with Mayer–Rokitansky–Kuster–Hauser syndrome and its successful laparoscopic management: a case-report and literature review
Authors: Jain Namita , Kriplani Isha , Sharma Seema , Hanumantaiya Shaily , Kriplani Alka
Published: June 2022

Mayer–Rokitansky–Küster–Hauser (MRKH) syndrome is the second most common cause of primary amenorrhea with an incidence of 1:4000–5000 women. It is characterized by aplasia or hypoplasia of the uterus and the upper two-thirds of the vagina with normal ovaries and tubes and a normal secondary sexual characteristics. The occurrence of leiomyoma is common but it is rare to have leiomyoma in uterine remnant in MRKH syndrome. Although few cases of MRKH syndrome with leiomyoma have been reported in the literature, none presented with urinary retention. Here, we report a case of 28-year-old women who presented with urinary retention that unmasked deeply embedded huge fibroids in pelvis arising from a rudimentary uterine horns and its safe management via laparoscopic approach.
Management of plant central metabolism by SnRK1 protein kinases
Authors: Peixoto Bruno , Baena-González Elena
Published: June 2022

SUCROSE NON-FERMENTING1 (SNF1)-RELATED KINASE 1 (SnRK1) is an evolutionarily conserved protein kinase with key roles in plant stress responses. SnRK1 is activated when energy levels decline during stress, reconfiguring metabolism and gene expression to favour catabolism over anabolism, and ultimately to restore energy balance and homeostasis. The capacity to efficiently redistribute resources is crucial to cope with adverse environmental conditions and, accordingly, genetic manipulations that increase SnRK1 activity are generally associated with enhanced tolerance to stress. In addition to its well-established function in stress responses, an increasing number of studies implicate SnRK1 in the homeostatic control of metabolism during the regular day–night cycle and in different organs and developmental stages. Here, we review how the genetic manipulation of SnRK1 alters central metabolism in several plant species and tissue types. We complement this with studies that provide mechanistic insight into how SnRK1 modulates metabolism, identifying changes in transcripts of metabolic components, altered enzyme activities, or direct regulation of enzymes or transcription factors by SnRK1 via phosphorylation. We identify patterns of response that centre on the maintenance of sucrose levels, in an analogous manner to the role described for its mammalian orthologue in the control of blood glucose homeostasis. Finally, we highlight several knowledge gaps and technical limitations that will have to be addressed in future research aiming to fully understand how SnRK1 modulates metabolism at the cellular and whole-plant levels.
MaizeHEAT UP-REGULATED GENE 1plays vital roles in heat stress tolerance
Authors: Xie Chen , Yang Liu , Jia Guixian , Yan Kang , Zhang Shizhong , Yang Guodong , Wu Changai , Gai Yingping , Zheng Chengchao , Huang Jinguang
Published: June 2022

Increasing temperature is one of the major threats to maize growth and yield globally. Under heat stress conditions, intracellular protein homeostasis is seriously disturbed, leading to accumulation of abnormally folded proteins, especially in the endoplasmic reticulum (ER). Molecular chaperones are vital players in the renaturation process and in preventing protein aggregation. However, heat stress tolerance-associated chaperones are not well documented in maize. Here, we characterized the biological roles of HEAT UP-REGULATED GENE 1 (ZmHUG1) in maize. ZmHUG1 encodes a heat-inducible holdase-type molecular chaperone localized in the ER. Knockout mutant of ZmHUG1 exhibited remarkably enhanced sensitivity to heat stress. Accordingly, the zmhug1 mutant showed severe ER stress under high temperature. MAIZE PRENYLATED RAB ACCEPTOR 1.C1 (ZmPRA1.C1) was identified as a client of ZmHUG1, and heat-induced aggregation of ZmPRA1.C1 was accelerated in the zmhug1 mutant. Furthermore, the expression of ZmHUG1 was rapidly transactivated by ER stress sensor BASIC LEUCINE ZIPPER DOMAIN 60 (bZIP60) when heat stress occurred. This study reveals a ZmHUG1-based thermo-protective mechanism in maize.
The Arabidopsis thaliana–Fusarium oxysporum strain 5176 pathosystem: an overview
Authors: Wang Liu , Calabria Jacob , Chen Hsiang-Wen , Somssich Marc
Published: June 2022

Fusarium oxysporum is a soil-borne fungal pathogen of several major food crops. Research on understanding the molecular details of fungal infection and the plant’s defense mechanisms against this pathogen has long focused mainly on the tomato-infecting F. oxysporum strains and their specific host plant. However, in recent years, the Arabidopsis thaliana–Fusarium oxysporum strain 5176 (Fo5176) pathosystem has additionally been established to study this plant–pathogen interaction with all the molecular biology, genetic, and genomic tools available for the A. thaliana model system. Work on this system has since produced several new insights, especially with regards to the role of phytohormones involved in the plant’s defense response, and the receptor proteins and peptide ligands involved in pathogen detection. Furthermore, work with the pathogenic strain Fo5176 and the related endophytic strain Fo47 has demonstrated the suitability of this system for comparative studies of the plant’s specific responses to general microbe- or pathogen-associated molecular patterns. In this review, we highlight the advantages of this specific pathosystem, summarize the advances made in studying the molecular details of this plant–fungus interaction, and point out open questions that remain to be answered.
Possible jet reorientation in low-frequency radio structures of blazars
Authors: Pajdosz-Śmierciak Urszula , Śmierciak Bartosz , Jamrozy Marek
Published: June 2022

Blazars are active galactic nuclei (AGNs) observed at small viewing angles to the observer’s line of sight; hence most of them are compact sources in the radio regime. At low radio frequencies, we may observe additional extended structures, considered to be lobes propagating directly towards Earth. However, there are rare cases of blazars whose morphologies are interpreted in the framework of an episodic jet activity with a reorientation of the jet axis. In this paper, we aim to find other candidates for sources of this kind and present Low Frequency Array (LOFAR) observations of seven blazars with rare morphologies composed of kiloparsec-scale jets and elongated multihundred kiloparsec-scale features. Based on their radio images and physical parameters, we propose that jets could change the direction of propagation out of the sky plane during the lifetime of at least some of these sources and with such we may observe morphologically different AGN types simultaneously, similar to the previously discovered blazar SBS B1646+499. Several plausible origin scenarios are presented, including fast realignment and precession. We also derive the 144 MHz fluxes of the compact and extended structures, the total radio luminosities, the spectral indices for the diffuse haloes, and the core-dominance parameters. We also discuss the possibility that these objects are actually giant blazars with rare Fanaroff–Riley I (FR I) morphology. The previously discovered blazar SBS B1646+499 should be classified as a giant independently of its inclination with regard to the observer.