bluuebunny/crossref_metadata_2025 huggingface dataset:
Utility of Fetal Cardiac Resonance Imaging in Prenatal Clinical Practice: Current State of the Art
Authors: Pozza Alice , Reffo Elena , Castaldi Biagio , Cattapan Irene , Avesani Martina , Biffanti Roberta , Cavaliere Annachiara , Cerutti Alessia , Di Salvo Giovanni
Published: November 2023

The field of prenatal cardiac imaging has revolutionized the way we understand and manage congenital heart diseases (CHD) in the developing fetus. In the prenatal period, cardiac imaging plays a pivotal role in the diagnostic pathway, from screening to classification and follow-up of CHD. The ability to visualize the fetal heart in utero allows healthcare professionals to detect abnormalities early, thus enabling timely interventions and informed decision-making processes for both the mother and the medical team. Early CHD detection improves preparation for delivery, postnatal care, and postnatal outcomes. Advancements in medical technology and imaging techniques have provided clinicians with insights into the fascinating workings of the fetal heart. Several imaging modalities have proven to be helpful in this field, with echocardiography undoubtedly representing the primary modality for evaluating the fetus. By providing detailed anatomical and functional information, fetal cardiac magnetic resonance (CMR) imaging contributes to better prenatal counseling and enhances the coordination of care between obstetricians, maternal–fetal medicine specialists, and pediatric cardiologists. Shortcomings of fetal CMR are due to technical concerns related to the intrauterine position of the fetus and subsequent challenges to following a standard scan protocol. The aim of this paper was to revise the current state-of-the-art in the field of fetal CMR and its clinical applications and to delve into methods, challenges, and future directions of fetal CMR in prenatal imaging.
Update on the Pathology of Pediatric Liver Tumors: A Pictorial Review
Authors: Bhagat Priyanka , Vij Mukul , Raju Lexmi Priya , Gowrishankar Gowripriya , Menon Jagadeesh , Shanmugam Naresh , Kaliamoorthy Ilankumaran , Rammohan Ashwin , Rela Mohamed
Published: November 2023

Liver tumors in children are uncommon and show remarkable morphologic heterogeneity. Pediatric tumors may arise from either the epithelial or mesenchymal component of the liver and rarely may also show both lines of differentiation. Both benign and malignant liver tumors have been reported in children. The most common pediatric liver tumors by age are benign hepatic infantile hemangiomas in neonates and infants, malignant hepatoblastoma in infants and toddlers, and malignant hepatocellular carcinoma in teenagers. Here, we provide an up-to-date review of pediatric liver tumors. We discuss the clinical presentation, imaging findings, pathology, and relevant molecular features that can help in the correct identification of these tumors, which is important in managing these children.
Analysis of the Chaotic Component of Photoplethysmography and Its Association with Hemodynamic Parameters
Authors: Xing Xiaoman , Dong Wen-Fei , Xiao Renjie , Song Mingxuan , Jiang Chenyu
Published: November 2023

Wearable technologies face challenges due to signal instability, hindering their usage. Thus, it is crucial to comprehend the connection between dynamic patterns in photoplethysmography (PPG) signals and cardiovascular health. In our study, we collected 401 multimodal recordings from two public databases, evaluating hemodynamic conditions like blood pressure (BP), cardiac output (CO), vascular compliance (C), and peripheral resistance (R). Using irregular-resampling auto-spectral analysis (IRASA), we quantified chaotic components in PPG signals and employed different methods to measure the fractal dimension (FD) and entropy. Our findings revealed that in surgery patients, the power of chaotic components increased with vascular stiffness. As the intensity of CO fluctuations increased, there was a notable strengthening in the correlation between most complexity measures of PPG and these parameters. Interestingly, some conventional morphological features displayed a significant decrease in correlation, indicating a shift from a static to dynamic scenario. Healthy subjects exhibited a higher percentage of chaotic components, and the correlation between complexity measures and hemodynamics in this group tended to be more pronounced. Causal analysis showed that hemodynamic fluctuations are main influencers for FD changes, with observed feedback in most cases. In conclusion, understanding chaotic patterns in PPG signals is vital for assessing cardiovascular health, especially in individuals with unstable hemodynamics or during ambulatory testing. These insights can help overcome the challenges faced by wearable technologies and enhance their usage in real-world scenarios.
Semantic Web Services Ingestion in a Process Mining Framework
Authors: Redavid Domenico , Ferilli Stefano
Published: November 2023

Process mining can be applied to systems for the management of Workflow, Business Processes and, in general, Process-Aware Information to discover and analyse implicit processes. In recent times, semantic interoperability has also become of crucial importance in the area of business processes. In particular, interoperability enables the discovery of new knowledge about processes by exploiting automatic reasoning on information originating from external formal descriptions. To this end, the use of Semantic Web technologies could be one possible solution. Given the different paradigms underpinning the two fields of research, adaptations are needed to realise this solution. In this paper, a possible mapping between Inductive Logic Programming and Semantic Web rules is proposed to discover additional knowledge that can be integrated into the process mining techniques outcomes.
Electrical Performance Compensation of Reflector Antenna Based on Sub-Reflector Array
Authors: Zhang Jie , Kong Xiangpeng , Zhang Chuyun , Gao Yifan , He Qiang
Published: November 2023

Large high-frequency reflector antennas typically operate outdoors and are impacted by environmental factors such as wind, rain, snow, solar radiation, etc. These conditions cause the antenna structures to distort, which, in turn, affects electrical performance. The observation performance of the antenna is seriously affected. To solve this problem, based on the active control of sub-reflector arrays, an optimization method for sub-reflector arrays and an active compensation method for electrical performance are proposed. Through real-time regulation of the phase plane of the distorted aperture field, the electrical performance of the antenna can be compensated for. The research object, a 65 m Cassegrain dual-reflector antenna, is designed in this work using a sub-reflector with an aperture of 6.1 m. The form and posture of the sub-reflector are comprehensively changed by adjusting the position of the sub-reflector panel and the overall movement of the sub-reflector, and the phase inaccuracy of the aperture field brought by the deformation of the main reflector is compensated for. The simulation results demonstrate that the maximum gain loss can be reduced from 0.44315 dB to 0.0803 dB and the maximum point error can be reduced from 0.00143° to 0.00047° under wind load with an average wind speed of 12 m/s using the active sub-reflector array adjustment strategy proposed.
Implementation of Blockchain Technology in Waste Management
Authors: Bułkowska Katarzyna , Zielińska Magdalena , Bułkowski Maciej
Published: November 2023

Implementing blockchain technology in waste management is a novel approach to environmental sustainability and accountability challenges in our modern world. Blockchain, a technology that enables decentralized and immutable ledgers, is now being re-imagined as a tool to revolutionize waste management. This innovative approach aims to improve waste management transparency, traceability, and efficiency, resulting in significant environmental and economic benefits. In traditional waste management systems, the tracking and disposal of waste materials are not transparent and can be vulnerable to fraud, mismanagement, and inefficiency. Blockchain technology provides a secure and transparent platform for recording every step in the waste management lifecycle, from waste generation to collection, transportation, recycling, or disposal. Every transaction in the blockchain is recorded in a tamper-proof manner, enabling real-time monitoring and verification of waste-related data. This paper introduces the concept of using blockchain technology in waste management. The main goal of this work is to show the implementation of blockchain technology in an existing waste management company, using smart contracts in the recycling process to provide transparency. Also, the digital product passport was redefined in terms of circular economy and waste recycling.
Determination of the Effect of a Thermal Curtain Used in a Greenhouse on the Indoor Climate and Energy Savings
Authors: Boyacı Sedat , Atilgan Atilgan , Kocięcka Joanna , Liberacki Daniel , Rolbiecki Roman , Jagosz Barbara
Published: November 2023

In order to reduce the impact of outdoor extreme weather events on crop production in winter, energy saving in greenhouses that are regularly heated is of great importance in reducing production costs and carbon footprints. For this purpose, the variations in indoor temperature, relative humidity and dew point temperature in the vertical direction (2 m, 4 m, 5.7 m) of thermal curtains in greenhouses were determined. In addition, depending on the fuel used, the curtains’ effects on heat energy consumption, heat transfer coefficient, carbon dioxide equivalents released to the atmosphere and fuel cost were investigated. To reach this goal, two greenhouses with the same structural features were designed with and without thermal curtains. As a result of the study, the indoor temperature and relative humidity values in the greenhouse with a thermal curtain increased by 1.3 °C and 10% compared to the greenhouse without a thermal curtain. Thermal curtains in the greenhouse significantly reduced fuel use (59.14–74.11 m3·night−1). Considering the heat energy consumption, the average heat energy consumption was 453.7 kWh·night−1 in the greenhouse with a curtain, while it was 568.6 kWh·night−1 in the greenhouse without a curtain. The average heat transfer coefficient (U) values were calculated at 2.87 W·m−2 °C with a thermal curtain and 3.63 W·m−2 °C without a thermal curtain greenhouse. In the greenhouse, closing the thermal curtain at night resulted in heat energy savings of about 21%, related to the decrease in U values. The use of a thermal curtain in the greenhouse reduced the amount of CO2 released to the atmosphere (116.6–146.1 kg·night−1) and fuel cost (USD 21.3–26.7·night−1). To conclude, extreme weather events in the outdoor environment adversely affect the plants grown in greenhouses where cultivation is performed out of season. A thermal curtain, used to reduce these adverse effects and the amount of energy consumed, is essential in improving indoor climate conditions, providing more economical greenhouse management and reducing the CO2 released into the atmosphere due to fuel use.
Research Progress on Aging Prediction Methods for Fuel Cells: Mechanism, Methods, and Evaluation Criteria
Authors: Tian Zhuang , Wei Zheng , Wang Jinhui , Wang Yinxiang , Lei Yuwei , Hu Ping , Muyeen S. M. , Zhou Daming
Published: November 2023

Due to the non-renewable nature and pollution associated with fossil fuels, there is widespread research into alternative energy sources. As a novel energy device, a proton exchange membrane fuel cell (PEMFC) is considered a promising candidate for transportation due to its advantages, including zero carbon emissions, low noise, and high energy density. However, the commercialization of fuel cells faces a significant challenge related to aging and performance degradation during operation. In order to comprehensively address the issue of fuel cell aging and performance decline, this paper provides a detailed review of aging mechanisms and influencing factors from the perspectives of both the PEMFC system and the stack. On this basis, this paper offers targeted solutions to degradation issues stemming from various aging factors and presents research on aging prediction methods to proactively mitigate aging-related problems. Furthermore, to enhance prediction accuracy, this paper categorizes and analyzes the degradation index and accuracy evaluation criteria commonly employed in the existing fuel cell aging research. The results indicate that specific factors leading to aging-related failures are often addressed via targeted solving methods, corresponding to specific degradation indexes. The significance of this study lies in the following aspects: (1) investigating the aging factors in fuel cells and elucidating the multiple aging mechanisms occurring within fuel cells; (2) proposing preventive measures, solutions, and aging prediction methods tailored to address fuel cell aging issues comprehensively, thereby mitigating potential harm; and (3) summarizing the degradation index and accuracy evaluation standards for aging prediction, offering new perspectives for resolving fuel cell aging problems.
Experimental Study on the Improvement of the Film Cooling Effectiveness of Various Modified Configurations Based on a Fan-Shaped Film Cooling Hole on a Flat Plate
Authors: Kim Seokmin , Lee DongEun , Kang Young Seok , Rhee Dong-Ho
Published: November 2023

Modern gas turbines have evolved by increasing the turbine inlet temperature (TIT) to improve performance. This development has led to a demand for cooling techniques. Among these, the film cooling, which involves injecting compressed air through holes on the turbine surface, is a prominent cooling technique used to protect the turbine surface. In this study, a comparative analysis is conducted between the conventional fan-shaped film cooling hole, primarily used in film cooling techniques, and modified shapes achieved by altering the geometry of the film cooling hole based on a fan-shaped hole to assess and compare the cooling performance on a flat plate surface. The adiabatic film cooling effectiveness was measured for three film cooling holes, the Baseline of a 7-7-7 fan-shaped film cooling hole, namely, Staircase, which had a double-step at the hole exit, and Compound Expansion, which had an additional expanded flow path at the hole leading edge. The used measurement technique was the pressure-sensitive paint (PSP) technique, using nitrogen gas as the foreign gas, and experiments were conducted at a density ratio of 1.0 and blowing ratios ranging from 0.5 to 2.0. The results reveal that the modified holes featured wider lateral expansion at the hole exits, resulting in a broader distribution of the cooling effectiveness in the lateral direction compared to the Baseline. The Staircase shows a better performance, although an overall cooling effectiveness trend similar to that of the Baseline. Furthermore, the Compound Expansion demonstrates an enhancement in the cooling performance with an increased blowing ratio, notably achieving nearly double the cooling effectiveness compared to that of the Baseline at a blowing ratio of 2.0.
Comparison of Technical and Operational Conditions of Traditional and Modern Charcoal Kilns: A Case Study in Italy
Authors: Mencarelli Alessio , Cavalli Raffaele , Greco Rosa , Grigolato Stefano
Published: November 2023

The global charcoal trade is steadily growing, with high-income countries importing significant quantities of this material from regions where its production is often associated with severe environmental issues, including forest overexploitation, illegal logging, and environmental pollution. Promoting local charcoal production in high-income countries is crucial to addressing these challenges. In this study, we have chosen to focus on the European context, specifically emphasizing Italy as a case study. Our study aimed to comprehensively compare five distinct charcoal production systems, including both traditional and modern solutions, with a specific focus on evaluating the quality of the resulting charcoal. Additionally, improvements were evaluated to enhance production efficiency. Traditional systems cannot satisfy production requests, resulting in inefficiencies in manpower, costs, times, and yield. Conversely, recent innovations consider mobile and stationary kiln prototypes. Mobile kilns offer flexibility and cost savings but require operator expertise, limit automation, and have long cycles. In contrast, stationary systems operate continuously, increasing productivity and efficiency, despite higher investment costs. Notably, charcoal quality showed minimal differences. These findings highlighted the potential of new technologies to enhance efficiency, reduce cost and environmental impact, and promote sustainable charcoal production.
Coordinated Planning of Power Systems under Uncertain Characteristics Based on the Multilinear Monte Carlo Method
Authors: Zhao Lang , Zeng Yuan , Li Yizheng , Peng Dong , Wang Yao
Published: November 2023

The randomness of the power supply side and the load side of comprehensive energy systems is increasingly prominent. It is very difficult to meet demand through traditional planning methods. To solve this problem, this paper explores the coordinated planning of a power system under uncertain characteristics using the multilinear Monte Carlo method. The uncertain characteristic model and probability density function of the system’s power supply side and load side are established. Taking the optimal operating cost and the maximum wind power consumption as the system planning objectives, a system coordination planning scheme is established, and it is solved by multilinear Monte Carlo simulation. The superiority of this method is verified by taking the modified IEEE 39-bus test system as an example. This method can provide a reference for system planning.
Iron Competition as an Important Mechanism of Pulcherrimin-Producing Metschnikowia sp. Strains for Controlling Postharvest Fungal Decays on Citrus Fruit
Authors: Wang Shupei , Tan Zhimei , Wang Chenshu , Liu Wenqing , Hang Fangxue , He Xuemei , Ye Dongqing , Li Li , Sun Jian
Published: November 2023

This study identified and tested fruit-isolated Metschnikowia yeasts against three major postharvest citrus pathogens, namely, Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, and further evaluated the impact of FeCl3 on the biocontrol efficiency of pulcherrimin-producing M. pulcherrima strains. Based on the characterization of the pigmented halo surrounding the colonies and the analysis of the D1/D2 domain of 26S rDNA, a total of 46 Metschnikowia sp. were screened and identified. All 46 Metschnikowia strains significantly inhibited the hyphal growth of Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, and effectively controlled the development of green mold, blue mold and sour rot of citrus fruit. The introduction of exogenous FeCl3 at certain concentrations did not significantly impact the pulcherriminic acid (PA) production of pigmented M. pulcherrima strains, but notably diminished the size of pigmented zones and the biocontrol efficacy against the three pathogens. Iron deficiency sensitivity experiments revealed that P. digitatum and P. italicum exhibited higher sensitivity compared to G. citri-aurantii, indicating that iron dependence varied among the three pathogens. These results suggested that M. pulcherrima strains, capable of producing high yields of PA, possessed great potential for use as biocontrol agents against postharvest citrus diseases. The biocontrol efficacy of these yeasts is mainly attributed to their ability to competitively deplete iron ions in a shared environment, with the magnitude of their pigmented halo directly correlating to their antagonistic capability. It is worth noting that the level of sensitivity of pathogens to iron deficiency might also affect the biocontrol effect of pulcherrimin-producing M. pulcherrima.
Comparative Assessment of Lignan, Tocopherol, Tocotrienol and Carotenoids in 40 Selected Varieties of Flaxseed (Linum usitatissimum L.)
Authors: Wu Zhimin , Li Yazhi , Qiu Huajiao , Long Songhua , Zhao Xinlin , Wang Yufu , Guo Xinbo , Baitelenova Aliya , Qiu Caisheng
Published: November 2023

Multiple varieties of flaxseeds have been identified in the world, yet the relationship between these varieties, their agronomic traits, and their seeds’ quality remains unclear. This study aimed to determine the level of lignan, vitamins and carotenoids in 40 selected flaxseed varieties, and the relationship between varieties, agronomic traits, and seed quality was investigated. In this study, notably, fiber flax variety No. 225 exhibited the highest lignan content among all tested seeds. Additionally, oil variety No. 167 demonstrated the highest level of α-tocotrienol (α-T3), β-tocopherol (β-T), γ-tocotrienol (γ-T3), and β-carotene (β-Car.). Conversely, intermediate flax variety No. 16 displayed the highest content of α-tocopherol (α-T), but lowest content of lutein (Lut.), zeaxanthin (Zea.), β-carotene (β-Car.), and total carotenoids (Total Car.). Furthermore, a correlation was observed between petal color with the lignan, while a strong correlation has been explored in seed yield, seed type, plant natural height, and fiber content in straw. Nevertheless, further investigation is required to elucidate the internal relationship between varieties with compositions.
Polyphenol Composition, Antioxidant Capacity and Xanthine Oxidase Inhibition Mechanism of Furong Plum Fruits at Different Maturity Stages
Authors: Zheng Zhipeng , Wu Li , Deng Wei , Yi Kexin , Li Yibin
Published: November 2023

An experiment was conducted on the polyphenol content, flavonoid content, anthocyanin content, and antioxidant capacity of Furong plum (Prunus salicina Lindl. cv. “furong”) at different maturity stages to determine the most suitable maturity stage. The inhibition of plum polyphenols on xanthine oxidase (XOD) was measured, and its kinetics were studied to reveal the inhibitory mechanism. The experimental results showed that the polyphenol, flavonoid and anthocyanin contents of plums at the ripe stage were the highest, reaching 320.46 mg GAE/100 g FW, 204.21 mg/100 g FW, and 66.24 mg/100 g FW, respectively, in comparison those of the plums at the immature and mid-ripe stages. The antioxidant capacity of the ripe plums was stronger than it was during the other stages of the plums growth. Among them, the total polyphenols of the ripe plums exhibited the strongest antioxidant capacity (IC50 values against DPPH and hydroxyl radicals were 28.19 ± 0.67 μg/mL and 198.16 ± 7.55 μg/mL, respectively), which was between the antioxidant capacity of the free polyphenols and bound polyphenols. The major phenolic monomer compounds of plum polyphenols were flavan-3-ols (epicatechin, catechin, proanthocyanidin, and procyanidin B2), flavonols (myricetin), and phenolic acids (chlorogenic acid, ferulic acid, and protocatechuic acid). Additionally, plum polyphenols exhibited a strong inhibitory effect on XOD, with an IC50 value of 77.64 μg/mL. The inhibition kinetics showed that plum polyphenols are mixed-type inhibitors that inhibit XOD activity and that the inhibition process is reversible. The calculated values of Ki and α were 16.53 mmol/L and 0.26, respectively.
Current State of Milk, Dairy Products, Meat and Meat Products, Eggs, Fish and Fishery Products Authentication and Chemometrics
Authors: Smaoui Slim , Tarapoulouzi Maria , Agriopoulou Sofia , D’Amore Teresa , Varzakas Theodoros
Published: November 2023

Food fraud is a matter of major concern as many foods and beverages do not follow their labelling. Because of economic interests, as well as consumers’ health protection, the related topics, food adulteration, counterfeiting, substitution and inaccurate labelling, have become top issues and priorities in food safety and quality. In addition, globalized and complex food supply chains have increased rapidly and contribute to a growing problem affecting local, regional and global food systems. Animal origin food products such as milk, dairy products, meat and meat products, eggs and fish and fishery products are included in the most commonly adulterated food items. In order to prevent unfair competition and protect the rights of consumers, it is vital to detect any kind of adulteration to them. Geographical origin, production methods and farming systems, species identification, processing treatments and the detection of adulterants are among the important authenticity problems for these foods. The existence of accurate and automated analytical techniques in combination with available chemometric tools provides reliable information about adulteration and fraud. Therefore, the purpose of this review is to present the advances made through recent studies in terms of the analytical techniques and chemometric approaches that have been developed to address the authenticity issues in animal origin food products.
Sea Buckthorn Pretreatment, Drying, and Processing of High-Quality Products: Current Status and Trends
Authors: Zhang Xuetao , Li Mengqing , Zhu Lichun , Geng Zhihua , Liu Xinyu , Cheng Zheyu , Zhao Mengxu , Zhang Qian , Yang Xuhai
Published: November 2023

Sea buckthorn is a kind of berry rich in nutritional and industrial value. Due to its thin skin, juicy pulp, and short shelf life, it is usually preserved via freezing methods or directly processed into sea buckthorn puree after harvest. It can also be dried and processed into products such as dried sea buckthorn fruit, freeze-dried sea buckthorn powder, and sea buckthorn oil. This review, therefore, provides an overview of the existing state of drying and high-quality processing of sea buckthorn. The effects of different pretreatment and drying techniques on the drying characteristics and quality of sea buckthorn and the existing problems of superior-quality processing of sea buckthorn products are summarised. The development trend of sea buckthorn drying methods and the ways to achieve high-quality processing of sea buckthorn products are indicated. These ways are mainly related to the following: (1) The application of combined pretreatment and drying techniques to find a balance between economy, ecology, and efficiency; (2) Introducing new online measurement and control technology into drying equipment; (3) Optimising the existing process to form a complete sea buckthorn industrial chain and develop the sea buckthorn deep-processing industry.
Design of Control Circuit for Tunable Semiconductor Laser for Fiber Sensing
Authors: Kong Ling , Lv Wenjie , He Haijing , Yuan Yibo , Du Libin
Published: November 2023

Semiconductor lasers have garnered significant prominence in diverse domains, including fiber optic communication and precision measurement, owing to their remarkable attributes such as compact size, lightweight construction, broad wavelength range, and tunability. Among these lasers, tunable semiconductor lasers assume a pivotal role in fiber Bragg grating demodulation systems, as the stability of their output wavelength and power directly influences the overall performance of the demodulation system. Ensuring the steadfastness of the output power and emission wavelength necessitates the provision of a stable driving current and the maintenance of a consistent operating temperature. Consequently, a specialized driver circuit necessitates meticulous design and implementation. In this investigation, a novel STM32 microcontroller-based tunable laser control circuit was meticulously developed to meet the practical requisites of fiber Bragg grating sensor demodulation. Leveraging the advanced capabilities of the MAX5113 current control chip and the MAX1978 temperature control chip, a dedicated circuit for constant current driving and temperature regulation of the tunable semiconductor laser was meticulously devised. Additionally, the design incorporates cutting-edge components, including a photodetector and an ADC conversion module, to seamlessly fulfill the intricate demands of the fiber Bragg grating demodulation system. The conclusive experimental results conclusively demonstrate the excellent stability of the output current produced by the constant current driving circuit, the minimal fluctuations observed in laser temperature, and the remarkable tunability of the laser’s output wavelength within the precise range of 1525 to 1550 nm. Notably, the wavelength fluctuations are confined to an impressively narrow margin of just 3 pm, providing definitive evidence that the design fully satisfies the practical requirements.
Factors Influencing Sexuality in Late Adolescence: A Qualitative Study on Heterosexual Adolescents’ Perspectives
Authors: Fernández-Medina Isabel María , Ramal-Gea Miguel Angel , Ruíz-Fernández María Dolores , Granero-Molina José , Jiménez-Lasserrotte María del Mar , Ventura-Miranda María Isabel
Published: November 2023

(1) Background: Sexuality is influenced by the school, family, and social contexts. All of these play a crucial role in promoting adolescents’ sexual health and well-being. However, little is known about the factors that have an impact on sexuality in late adolescence. The aim of this study was to explore and describe the perspectives of late adolescents on the factors that influence their sexual lives. (2) Methods: A qualitative study with a descriptive design was carried out. Thirteen interviews were conducted. Data were audio-recorded, transcribed, and categorized into themes and subthemes. (3) Results: The results of our study offer support for the importance of sexual aspects in the lives of late adolescents who identify as heterosexual. (4) Conclusions: The most influential environments in late adolescent sexuality are the social, family, school, and peer contexts.
An Outport for Gedi?—Archaeological Survey in Mida Creek, Kenya
Authors: Bita Caesar , Forsythe Wes
Published: November 2023

Communities of the East African coast have had a long history of international interaction. Swahili maritime networks linked African port towns across the Indian Ocean seaboard, thus enabling merchandise to reach the hinterlands of the continent. One of these Swahili towns is the ancient city of Gedi, located on the central Kenyan coast near the medieval town of Malindi. Located inshore, 6 km from the main ocean to the southeast and 3 km from Mida Creek to the southwest, Gedi’s lack of seaward connectivity has perplexed scholars. To effectively function within the vibrant Indian Ocean maritime trade networks of the 10th to 16th centuries CE, Gedi needed access to the sea. Inevitably goods had to be transported overland from a port or landing place. The ‘MUCH to Discover in Mida Creek’ project carried out an archaeological survey around the Creek to form a broader understanding of the region’s maritime past. The study identified a range of sites that evidence past coastal activity. In particular, a concentration of contemporary house sites at Chafisi near the closest point on the Creek to Gedi would suggest it may have acted as an outport for the stone town.
Exploring the Potential of Olfactory Receptor Circulating RNA Measurement for Preeclampsia Prediction and Its Linkage to Mild Gestational Hypothyroidism
Authors: Hirata Andréa Harumy de Lima , Camargo Luiz Antônio de Jesus Rocha , Silva Valdelena Alessandra da , Almeida Robson José de , Bacigalupo Lucas dos Santos , Albejante Maria Clara , Curi Flavia Salomão d’Avila , Varela Patrícia , Martins Leonardo , Pesquero João Bosco , Delle Humberto , Camacho Cleber P.
Published: November 2023

Gestational hypothyroidism may lead to preeclampsia development. However, this pathophysiological is unknown. We expect to find a shared mechanism by comparing hypothyroidism and preeclampsia. From our transcriptome data, we recognized olfactory receptors as that fingerprint. The reduction of taste and smell in hypothyroid patients has been known for a long time. Therefore, we decided to look to the olfactory receptors and aimed to identify genes capable of predicting preeclampsia (PEC). Methods: An Ion Proton Sequencer (Thermo Fisher Scientific, Waltham, MA, USA) was used to construct the transcriptome databases. RStudio with packages Limma v.3.50.0, GEOquery v.2.62.2, and umap v.0.2.8.8 were used to analyze the differentially expressed genes in GSE149440 from the Gene Expression Omnibus (GEO). The 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) was used for RT-qPCR amplification of OR6X1 and OR4E2. Results: Our transcriptomic datasets analysis revealed 25.08% and 26.75% downregulated olfactory receptor (ORs) in mild nontreated gestational hypothyroidism (GHT) and PEC, respectively. In the GSE149440 GEO dataset, we found OR5H1, OR5T3, OR51A7, OR51B6, OR10J5, OR6C6, and OR2AG2 as predictors of early-onset PEC. We also evaluate two chosen biomarkers’ responses to levothyroxine. The RT-qPCR demonstrated a difference in OR6X1 and OR4E2 expression between GHT and healthy pregnancy (p < 0.05). Those genes presented a negative correlation with TSH (r: −0.51, p < 0.05; and r: −0.44, p < 0.05), a strong positive correlation with each other (r: 0.89; p < 0.01) and the levothyroxine-treated group had no difference from the healthy one. We conclude that ORs could be used as biomarkers at the beginning of gestation, and the downregulated ORs found in GHT may be improved with levothyroxine treatment.
Alkoxysilane-Mediated Decoration of Si Nanowires Vertical Arrays with Au Nanoparticles as Improved SERS-Active Platforms
Authors: Lo Faro Maria Josè , Ielo Ileana , Morganti Dario , Leonardi Antonio Alessio , Conoci Sabrina , Fazio Barbara , De Luca Giovanna , Irrera Alessia
Published: November 2023

The search for improved transducers to fabricate better-performing (bio)sensors is a challenging but rewarding endeavor aiming to better diagnose and treat diseases. In this paper, we report on the decoration of a dense vertical array of ultrathin silicon nanowires (Si NWs), produced by metal-assisted chemical etching, with 20 nm gold nanoparticles (Au NPs) for surface-enhanced Raman scattering (SERS) applications. To optimize the production of a uniform 3D SERS active platform, we tested different Si NW surface functionalizations with various alkoxysilanes before Au decoration. Scanning electron microscopy investigations confirm that Au NPs decorate both bare and (3-glycidiloxypropyl)trimethoxysilane (GPTMS)-modified Si NWs with a high surface coverage uniformity. The SERS response of the decorated NWs was probed using a model dye system (methylene blue; MB) at 633 and 785 nm excitation wavelengths. The GPTMS-modified NWs present the highest enhancements of 2.9 and 2.6 for the 450 cm−1 and 1625 cm−1 peaks under 785 nm excitation and of 10.8 and 5.3 for the 450 cm−1 and 1625 cm−1 peaks under 633 nm excitation. These results demonstrate the perspective role of Si NWs decorated with Au NPs as a low-cost 3D SERS platform.
Influence of Topography and Composition of Commercial Titanium Dental Implants on Cell Adhesion of Human Gingiva-Derived Mesenchymal Stem Cells: An In Vitro Study
Authors: Campos-Bijit Vanessa , Inostroza Nicolás Cohn , Orellana Rocío , Rivera Alejandro , Von Marttens Alfredo , Cortez Cristian , Covarrubias Cristian
Published: November 2023

The topography and composition of dental implant surfaces directly impact mesenchymal cell adhesion, proliferation, and differentiation, crucial aspects of achieving osseointegration. However, cell adhesion to biomaterials is considered a key step that drives cell proliferation and differentiation. The aim of this study was to characterize characterize the topography and composition of commercial titanium dental implants manufactured with different surface treatments (two sandblasted/acid-etched (SLA) (INNO Implants, Busan, Republic of Korea; BioHorizonsTM, Oceanside, CA, USA) and two calcium phosphate (CaP) treated (Biounite®, Berazategui, Argentina; Zimmer Biomet, Inc., Warsaw, IN, USA)) and to investigate their influence on the process of cell adhesion in vitro. A smooth surface implant (Zimmer Biomet, Inc.) was used as a control. For that, high-resolution methodologies such as scanning electron microscopy (SEM), X-ray dispersive spectroscopy (EDX), laser scanning confocal microscopy (LSCM), and atomic force microscopy (AFM) were employed. Protein adsorption and retromolar gingival mesenchymal stem cells (GMSCs) adhesion to the implant surfaces were evaluated after 48 h. The adherent cells were examined by SEM and LSCM for morphologic and quantitative analyses. ANOVA and Tukey tests (α = 0.05) were employed to determine statistical significance. SEM revealed that INNO, BioHorizonsTM, and Zimmer implants have an irregular surface, whereas Biounite® has a regular topography consisting of an ordered pattern. EDX confirmed a calcium and phosphate layer on the Biounite® and Zimmer surfaces, and AFM exhibited different roughness parameters. Protein adsorption and cell adhesion were detected on all the implant surfaces studied. However, the Biounite® implant with CaP and regular topography showed the highest protein adsorption capacity and density of adherent GMSCs. Although the Zimmer implant also had a CaP treatment, protein and cell adhesion levels were lower than those observed with Biounite®. Our findings indicated that the surface regularity of the implants is a more determinant factor in the cell adhesion process than the CaP treatment. A regular, nanostructured, hydrophilic, and moderately rough topography generates a higher protein adsorption capacity and thus promotes more efficient cell adhesion.
Akt, IL-4, and STAT Proteins Play Distinct Roles in Prostaglandin Production in Human Follicular Dendritic Cell-like Cells
Authors: Jeong Jihye , Choe Jongseon
Published: November 2023

This study aimed to explore the role of Akt protein in the induction and inhibition of prostaglandin (PG) in human follicular dendritic cell (FDC)-like cells. FDC-like cells and B cells were isolated from human tonsils. PG production was assessed using enzyme immunoassay, while the upstream cyclooxygenase-2 (COX-2) protein levels were measured using immunoblotting with FDC-like cells transfected with Akt siRNA to analyze the impact of Akt knockdown. The COX-2 expression and PG production induced with IL-1β were significantly increased by Akt knockdown. However, IL-1β did not significantly alter either total or phosphorylated Akt protein levels. Akt knockdown resulted in the augmentation of COX-2 expression induced by B cells, although the addition of B cells did not significantly modulate both total and phosphorylated Akt proteins. In contrast, IL-4 specifically exhibited a potent inhibitory effect on COX-2 protein induction and PG production via STAT6. The inhibitory activity of IL-4 was not hampered by Akt knockdown. Interestingly, COX-2 expression levels induced with IL-1β were markedly modulated with STAT1 and STAT3 knockdown. STAT1 silencing resulted in further augmentation of COX-2, whereas STAT3 silencing prohibited IL-1β from stimulating COX-2 expression. The current results suggest that Akt, IL-4, and STAT1 play inhibitory roles in PG production in FDC-like cells and expand our knowledge of the immune inflammatory milieu.
Identification of Metabolomic Markers in Frozen or Formalin-Fixed and Paraffin-Embedded Samples of Diffuse Glioma from Adults
Authors: Chardin David , Jing Lun , Chazal-Ngo-Mai Mélanie , Guigonis Jean-Marie , Rigau Valérie , Goze Catherine , Duffau Hugues , Virolle Thierry , Pourcher Thierry , Burel-Vandenbos Fanny
Published: November 2023

The aim of this study was to identify metabolomic signatures associated with the gliomagenesis pathway (IDH-mutant or IDH-wt) and tumor grade of diffuse gliomas (DGs) according to the 2021 WHO classification on frozen samples and to evaluate the diagnostic performances of these signatures in tumor samples that are formalin-fixed and paraffin-embedded (FFPE). An untargeted metabolomic study was performed using liquid chromatography/mass spectrometry on a cohort of 213 DG samples. Logistic regression with LASSO penalization was used on the frozen samples to build classification models in order to identify IDH-mutant vs. IDH-wildtype DG and high-grade vs low-grade DG samples. 2-Hydroxyglutarate (2HG) was a metabolite of interest to predict IDH mutational status and aminoadipic acid (AAA) and guanidinoacetic acid (GAA) were significantly associated with grade. The diagnostic performances of the models were 82.6% AUC, 70.6% sensitivity and 80.4% specificity for 2HG to predict IDH status and 84.7% AUC, 78.1% sensitivity and 73.4% specificity for AAA and GAA to predict grade from FFPE samples. Thus, this study showed that AAA and GAA are two novel metabolites of interest in DG and that metabolomic data can be useful in the classification of DG, both in frozen and FFPE samples.
Albumin/Mitotane Interaction Affects Drug Activity in Adrenocortical Carcinoma Cells: Smoke and Mirrors on Mitotane Effect with Possible Implications for Patients’ Management
Authors: Schiavon Aurora , Saba Laura , Catucci Gianluca , Petiti Jessica , Puglisi Soraya , Borin Chiara , Reimondo Giuseppe , Gilardi Gianfranco , Giachino Claudia , Terzolo Massimo , Lo Iacono Marco
Published: November 2023

Background: Mitotane is the only drug approved for the treatment of adrenocortical carcinoma (ACC). Although it has been used for many years, its mechanism of action remains elusive. H295R cells are, in ACC, an essential tool to evaluate drug mechanisms, although they often lead to conflicting results. Methods: Using different in vitro biomolecular technologies and biochemical/biophysical experiments, we evaluated how the presence of “confounding factors” in culture media and patient sera could reduce the pharmacological effect of mitotane and its metabolites. Results: We discovered that albumin, the most abundant protein in the blood, was able to bind mitotane. This interaction altered the effect of the drug by blocking its biological activity. This blocking effect was independent of the albumin source or methodology used and altered the assessment of drug sensitivity of the cell lines. Conclusions: In conclusion, we have for the first time demonstrated that albumin does not only act as an inert drug carrier when mitotane or its metabolites are present. Indeed, our experiments clearly indicated that both albumin and human serum were able to suppress the pharmacological effect of mitotane in vitro. These experiments could represent a first step towards the individualization of mitotane treatment in this rare tumor.
FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway
Authors: Wang Yaofeng , Wang Gengqiao , Hu Shaobo , Yin Chuanzheng , Zhao Peng , Zhou Xing , Shao Shuyu , Liu Ran , Hu Wenjun , Liu Gang Logan , Ke Wenbo , Song Zifang
Published: November 2023

Hepatocellular carcinoma (HCC) is a common malignant tumor with high mortality. Human phenylalanine tRNA synthetase (PheRS) comprises two α catalytic subunits encoded by the FARSA gene and two β regulatory subunits encoded by the FARSB gene. FARSB is a potential oncogene, but no experimental data show the relationship between FARSB and HCC progression. We found that the high expression of FARSB in liver cancer is closely related to patients’ low survival and poor prognosis. In liver cancer cells, the mRNA and protein expression levels of FARSB are increased and promote cell proliferation and migration. Mechanistically, FARSB activates the mTOR complex 1 (mTORC1) signaling pathway by binding to the component Raptor of the mTORC1 complex to play a role in promoting cancer. In addition, we found that FARSB can inhibit erastin-induced ferroptosis by regulating the mTOR signaling pathway, which may be another mechanism by which FARSB promotes HCC progression. In summary, FARSB promotes HCC progression and is associated with the poor prognosis of patients. FARSB is expected to be a biomarker for early screening and treatment of HCC.
Application Value of Antimicrobial Peptides in Gastrointestinal Tumors
Authors: Liu Qi , Wang Lei , He Dongxia , Wu Yuewei , Liu Xian , Yang Yahan , Chen Zhizhi , Dong Zhan , Luo Ying , Song Yuzhu
Published: November 2023

Gastrointestinal cancer is a common clinical malignant tumor disease that seriously endangers human health and lacks effective treatment methods. As part of the innate immune defense of many organisms, antimicrobial peptides not only have broad-spectrum antibacterial activity but also can specifically kill tumor cells. The positive charge of antimicrobial peptides under neutral conditions determines their high selectivity to tumor cells. In addition, antimicrobial peptides also have unique anticancer mechanisms, such as inducing apoptosis, autophagy, cell cycle arrest, membrane destruction, and inhibition of metastasis, which highlights the low drug resistance and high specificity of antimicrobial peptides. In this review, we summarize the related studies on antimicrobial peptides in the treatment of digestive tract tumors, mainly oral cancer, esophageal cancer, gastric cancer, liver cancer, pancreatic cancer, and colorectal cancer. This paper describes the therapeutic advantages of antimicrobial peptides due to their unique anticancer mechanisms. The length, net charge, and secondary structure of antimicrobial peptides can be modified by design or modification to further enhance their anticancer effects. In summary, as an emerging cancer treatment drug, antimicrobial peptides need to be further studied to realize their application in gastrointestinal cancer diseases.
G6PD Orchestrates Genome-Wide DNA Methylation and Gene Expression in the Vascular Wall
Authors: Signoretti Christina , Gupte Sachin A.
Published: November 2023

Recent advances have revealed the importance of epigenetic modifications to gene regulation and transcriptional activity. DNA methylation, a determinant of genetic imprinting and the de novo silencing of genes genome-wide, is known to be controlled by DNA methyltransferases (DNMT) and demethylases (TET) under disease conditions. However, the mechanism(s)/factor(s) influencing the expression and activity of epigenetic writers and erasers, and thus DNA methylation, in healthy vascular tissue is incompletely understood. Based on our recent studies, we hypothesized that glucose-6-phosphate dehydrogenase (G6PD) is a modifier of DNMT and TET expression and activity and an enabler of gene expression. In the aorta of CRISPR-edited rats with the Mediterranean G6PD variant, we determined DNA methylation by whole-genome bisulfite sequencing, gene expression by RNA sequencing, and large artery stiffness by echocardiography. Here, we documented higher expression of Dnmt1, Dnmt3a, Tet2, and Tet3 in aortas from Mediterranean G6PDS188F variant (a loss-of-function single nucleotide polymorphism) rats than their wild-type littermates. Concomitantly, we identified 17,618 differentially methylated loci genome-wide (5787 hypermethylated loci, including down-regulated genes encoding inflammation- and vasoconstriction-causing proteins, and 11,827 hypomethylated loci, including up-regulated genes encoding smooth muscle cell differentiation- and fatty acid metabolism-promoting proteins) in aortas from G6PDS188F as compared to wild-type rats. Our results demonstrated that nitric oxide, which is generated in a G6PD-derived NADPH-dependent manner, increases TET and decreases DNMT activity. Further, we observed less large artery (aorta) stiffness in G6PDS188F as compared to wild-type rats. These results establish a noncanonical function of the wild-type G6PD and G6PDS188F variant in the regulation of DNA methylation and gene expression in healthy vascular tissue and reveal that the G6PDS188F variant contributes to reducing large artery stiffness.