bluuebunny/crossref_metadata_2025 huggingface dataset:
Leader Humility, and Subordinates’ Organizational Citizenship Behavior and Withdrawal Behavior: Exploring the Mediating Mechanisms of Subordinates’ Psychological Capital
Authors: Qian Xiaoye , Zhang Meijuan , Jiang Qiang
Published: April 2020

As a bottom-up leadership style, leader humility has received considerable attention from researchers. Among the abundant studies revealing the positive impact of leader humility on employees’ work attitude and behaviors, there is less knowledge on how leader humility influences subordinates’ organizational citizenship behavior (OCB) and withdrawal behavior. On the basis of the social information processing theory, this study proposed a cross-level mediation model and examined the direct impact of leader humility on subordinates’ OCB and withdrawal behavior. We also further explored the underlying psychological mechanism and examined the mediating effect of psychological capital on these relationships. Using a two-wave panel design and 274 employees’ questionnaire data, the empirical analysis found that: (1) leader humility was positively related to subordinates’ OCB and negatively related to subordinates’ withdrawal behavior; (2) leader humility was positively related to subordinates’ psychological capital; and (3) psychological capital played a cross-level mediating role in the leader humility-subordinates’ OCB relationship and the leader humility-subordinates’ withdrawal behavior relationship. Theoretical and practical implications, limitations, and suggestions for future research are also discussed.
Understanding the Underlying Psychosocial Determinants of Safe Food Handling among Consumers to Mitigate the Transmission Risk of Antimicrobial-Resistant Bacteria
Authors: Freivogel Claudia , H. M. Visschers Vivianne
Published: April 2020

In this study, we aimed to explore the psychosocial factors related to consumers’ safe food-handling behaviors to reduce the transmission risk of antimicrobial-resistant bacteria through food products. To this end, we investigated the extent to which the variables of the health action process approach (HAPA) and subjective norms can predict safe food handling by consumers. Data were collected from a representative sample of consumers belonging to the German-speaking part of Switzerland by administering a self-reporting questionnaire. The results showed that risk perception, self-efficacy, and positive outcome expectancy (i.e., the motivational phase of HAPA) were significant predictors of the intentions to handle food in a safe manner (see graphical abstract below). Additionally, in the volitional phase of HAPA, intention was found to be a significant predictor of safe food-handling behaviors. Contrary to expectations based on HAPA, action planning did not mediate the intention–behavior relationship. Only a small part of the variance in behavior was explained by coping planning and action control. The findings confirmed that the motivational phase of HAPA may be useful for determining safe food handling.
The Sacred Power of Beauty: Examining the Perceptual Effect of Buddhist Symbols on Happiness and Life Satisfaction in China
Authors: Qin Zhenzhen , Song Yao
Published: April 2020

The theoretical relationship between Buddhism and subjective well-being has gained much academic attention in recent decades. However, the prominent determinants of religiosity remain limited for researchers to understand a holistic picture of religion-informed subjective well-being, particularly in the context of Buddhism. This study has applied a quantitative survey to verify the impact of the aesthetic effects of the Buddhist gesture symbol on Chinese people’s subjective happiness through sequential mediators of life satisfaction and the perceived religiosity. The significance of this study is threefold. Firstly, it aims to enrich the current academic understanding of the religion-informed subjective well-being by introducing a new determinant of the Buddhist symbols. Secondly, the current study investigates the mechanism of how Buddhist symbols could influence happiness by analyzing the sequential mediating roles of religiosity and life satisfaction. Thirdly, this study empirically examines the topic in the context of China to confirm and underpin the theoretical relationship between Buddhism and subjective well-being in relevant research, which has previously focused on Western culture. Our results indicated that the aesthetic perception of the Buddhist gesture symbol positively influenced perceived happiness and life satisfaction. In addition, perceived religiosity and life satisfaction sequentially mediated the perceived happiness after seeing the Buddhist gesture symbol. Our findings contribute to the current academic understanding of religious symbols and their impacts on subjective well-being.
Welding Fumes, a Risk Factor for Lung Diseases
Authors: Riccelli Maria Grazia , Goldoni Matteo , Poli Diana , Mozzoni Paola , Cavallo Delia , Corradi Massimo
Published: April 2020

(1) Background: Welding fumes (WFs) are composed of fine and ultrafine particles, which may reach the distal airways and represent a risk factor for respiratory diseases. (2) Methods: In vitro and in vivo studies to understand WFs pathogenesis were selected. Epidemiological studies, original articles, review, and meta-analysis to examine solely respiratory disease in welders were included. A systematic literature search, using PubMed, National Institute for Occupational Safety and Health Technical Information Center (NIOSHTIC), and Web of Science databases, was performed. (3) Results: Dose, time of exposure, and composition of WFs affect lung injury. Inflammation, lung defense suppression, oxidative stress, DNA damage, and genotoxic effects were observed after exposure both to mild and stainless steel WFs. (4) Conclusions: The detection of lung diseases associated with specific occupational exposure is crucial as complete avoidance or reduction of the exposure is difficult to achieve. Further studies in the area of particle research may aid the understanding of mechanisms involved in welding-related lung disease and to expand knowledge in welding-related cardiovascular diseases.
How CSV and CSR Affect Organizational Performance: A Productive Behavior Perspective
Authors: Park Kwang O.
Published: April 2020

Background: This study aims to shed light on the mutually beneficial causal relationship between creating shared value (CSV) and corporate social responsibility (CSR) activities and how they affect productive behavior through work engagement. Many preceding studies showed that work engagement and organizational citizenship behaviors (OCB) play a major role in the relationship between CSV and CSR activities and the organization’s internal performance. This study classified product behavior into OCB, innovative behavior, and job performance, based on the literature review. Methods: The subjects of this study were companies listed in KOSPI, which is Korea’s representative securities market. The companies listed on KOSPI are Korea’s leading companies as designated by the Korean government and financial authorities based on industry representation, market representation, and liquidity. Results: This study supported many preceding studies that analyzed the causal relationship between CSV and CSR activities, as well as OCB. In addition, this study has significant implications for businesses since it presents the possibility of studying the relationships between various organizational performance factors such as innovative behavior and job performance. Conclusions: It is expected that this study will help companies find more effective ways to strengthen their competitive advantage from a theoretical and practical perspective.
Evaluation of an Alimentary Education Intervention on School Canteen Waste at a Primary School in Bari, Italy
Authors: Favuzzi Nicoletta , Trerotoli Paolo , Forte Maria Grazia , Bartolomeo Nicola , Serio Gabriella , Lagravinese Domenico , Vino Francesco
Published: April 2020

The “Love Food, Not Waste” project was conducted to train students on good food choices and evaluate food waste in school canteens. Teachers, parents and students were surveyed before and after training. Weights of both the served and wasted food were recorded for one week both before the educational intervention in February 2019 and after the educational intervention in March 2019, using the same menu. Students completed a food satisfaction questionnaire on the days the data were collected. For the first dish, the mean wastes per school were 1199 g before training and 1054 g after training. For the second dish, the mean wastes per school were 246 g before training and 220 g after training. For the side course, the means wastes per school were 663 g before training and 747 g after training. The results did not significantly differ among weeks or schools. Less food was wasted when boys judged the food’s general aspects like smell, taste and appearance as positive; more food was wasted when girls judged these factors as negative. Food waste monitoring is mandatory but does not always occur. Analyzing food waste relative to students’ food perceptions can help determine whether educational interventions can help reduce waste. Students’ satisfaction must also be considered.
Determinants of Ageism against Older Adults: A Systematic Review
Authors: Marques Sibila , Mariano João , Mendonça Joana , De Tavernier Wouter , Hess Moritz , Naegele Laura , Peixeiro Filomena , Martins Daniel
Published: April 2020

Ageism is a widespread phenomenon and constitutes a significant threat to older people’s well-being. Identifying the factors contributing to ageism is critical to inform policies that minimise its societal impact. In this systematic review, we gathered and summarised empirical studies exploring the key determinants of ageism against older people for a period of over forty years (1970–2017). A comprehensive search using fourteen databases identified all published records related to the umbrella concept of “ageism”. Reviewers independently screened the final pool to identify all papers focusing on determinants, according to a predefined list of inclusion and exclusion criteria. All relevant information was extracted and summarised following a narrative synthesis approach. A total of 199 papers were included in this review. We identified a total of 14 determinants as robustly associated with ageism. Of these, 13 have an effect on other-directed ageism, and one on self-directed ageism. The quality of contact with older people and the positive or negative presentation of older people to others emerged as the most robust determinants of other-directed ageism; self-directed ageism is mostly determined by older adults’ health status. Given the correlational nature of most studies included in this review, inferences on causality should be made cautiously.
Atrazine Removal from Municipal Wastewater Using a Membrane Bioreactor
Authors: Kamaz Mohanad , Jones Steven M. , Qian Xianghong , Watts Michael J. , Zhang Wen , Wickramasinghe S. Ranil
Published: April 2020

As the demand for potable water increases, direct potable reuse of wastewater is an attractive alternative method to produce potable water. However, implementation of such a process will require the removal of emerging contaminants which could accumulate in the drinking water supply. Here, the removal of atrazine, a commonly used herbicide, has been investigated. Using real and synthetic wastewater, as well as sludge from two wastewater treatment facilities in the United States in Norman, Oklahoma and Fayetteville, Arkansas, atrazine removal has been investigated. Our results indicate that about 20% of the atrazine is removed by adsorption onto the particulate matter present. Significant biodegradation of atrazine was only observed under aerobic conditions for sludge from Norman, Oklahoma. Next-generation sequencing of the activated sludge revealed the abundance of Noncardiac with known atrazine degradation pathways in the Norman aerobic sludge, which is believed to be responsible for atrazine biodegradation in our study. The detection of these bacteria could also be used to determine the likelihood of biodegradation of atrazine for a given wastewater treatment facility.
A Systematic Review of Network Studies Based on Administrative Health Data
Authors: Karim Shakir , Uddin Shahadat , Imam Tasadduq , Moni Mohammad Ali
Published: April 2020

Effective and efficient delivery of healthcare services requires comprehensive collaboration and coordination between healthcare entities and their complex inter-reliant activities. This inter-relation and coordination lead to different networks among diverse healthcare stakeholders. It is important to understand the varied dynamics of these networks to measure the efficiency of healthcare delivery services. To date, however, a work that systematically reviews these networks outlined in different studies is missing. This article provides a comprehensive summary of studies that have focused on networks and administrative health data. By summarizing different aspects including research objectives, key research questions, adopted methods, strengths and weaknesses, this research provides insights into the inherently complex and interlinked networks present in healthcare services. The outcome of this research is important to healthcare management and may guide further research in this area.
Sexually Harassing Behaviors from Patients or Clients and Care Workers’ Mental Health: Development and Validation of a Measure
Authors: Vincent-Höper Sylvie , Adler Mareike , Stein Maie , Vaupel Claudia , Nienhaus Albert
Published: April 2020

Although evidence reveals severe effects of sexual harassment on care workers’ mental health, there is a scarcity of studies that investigate care workers’ experiences of sexually inappropriate behavior from patients or clients. One reason for this lack of research is that validated measures that assess different types of sexual harassment experienced by employees working with patients or clients are lacking. In this study, we seek to establish a conceptual framework for investigating extraorganizational sexual harassment in healthcare work. Based on this theoretical framework, we developed and validated a measure for assessing sexually harassing behaviors from patients or clients. Data were gathered from heterogeneous samples of employees working in a variety of settings in healthcare. To evaluate the factorial structure of the measure, we conducted exploratory factor analysis (EFA) using a calibration sample (N = 179) and confirmatory factor analysis (CFA) using a cross-validation sample (N = 305). The construct validity of the measure was demonstrated by investigating relationships with indicators of care workers’ mental health. EFA revealed three factors, namely, nonverbal, verbal, and physical acts of sexual harassment. Examination of the measure comprising 14 items revealed acceptable internal consistencies and substantial correlations with indicators of care workers’ mental health. This study provides a useful and sound measure for assessing sexual harassment from patients or clients and paves the way for the development of a comprehensive theoretical framework for the assessment of sexual harassment. Furthermore, it facilitates future investigations of risk factors for sexual harassment and protective factors helping healthcare workers cope with sexual harassment from patients or clients.
Implicit Theories about Athletic Ability Modulate the Effects of If-Then Planning on Performance in a Standardized Endurance Task
Authors: Hirsch Anna , Bieleke Maik , Schüler Julia , Wolff Wanja
Published: April 2020

Muscular strength has a strong positive impact on cardiometabolic health and fitness. However, building up strength endurance requires effortful exercises. From a health perspective, it is important to understand which psychological strategies help people deal with straining exercise. Self-regulation strategies like if-then planning (also known as implementation intentions) appear particularly promising because they might directly alter how people deal with exercise-induced sensations. However, research on the effects of if-then planning on exercise performance has yielded mixed results so far. One possible reason for these inconsistent results is the lack of tailored interventions and the neglect of potential moderators. To address this, we investigated the efficacy of if-then plans that were tailored to perceived limits of endurance performance (i.e., perceptions of exertion versus pain). In addition, we investigated the effects of these tailored if-then plans while taking into account the potentially moderating effects of individual differences in implicit theories. Specifically, we were interested in the role of implicit theories about athletic performance (i.e., entity versus incremental beliefs) and about the limitation of athletic performance by mental versus physical factors (i.e., mind-over-body beliefs). N = 66 male students (age: M = 25.8 years, SD = 3.2) performed a static muscular endurance task twice (measurement: baseline task vs. main task) and were randomly assigned to a goal or an implementation intention condition. They were instructed to hold two intertwined rings for as long as possible while avoiding contacts between them (measure of performance: time-to-failure and errors). After the baseline task, participants were either given an implementation intention or were simply asked to rehearse the task instructions. The content of the instruction depended on whether they ascribed ultimate baseline task termination to perceptions of exertion or pain. After the main task, implicit theories on athletic ability were assessed. No differences in performance emerged between conditions. In the implementation intention condition, however, stronger entity beliefs were associated with increasing time-to-failure when participants planned to ignore exertion but with decreasing time-to-failure when they planned to ignore pain. This pattern of results was reversed with regard to mind-over-body beliefs. These findings indicate that the efficacy of psychological strategies hinges on recreational athletes’ beliefs regarding athletic performance.
Sitting Posture, Sagittal Spinal Curvatures and Back Pain in 8 to 12-Year-Old Children from the Region of Murcia (Spain): ISQUIOS Programme
Authors: Sainz de Baranda Pilar , Cejudo Antonio , Martínez-Romero María Teresa , Aparicio-Sarmiento Alba , Rodríguez-Ferrán Olga , Collazo-Diéguez Mónica , Hurtado-Avilés José , Andújar Pilar , Santonja-Medina Fernando
Published: April 2020

To explore sagittal spinal alignment and pelvic disposition of schoolchildren in a slump sitting position is needed in order to establish preventive educational postural programs. The purposes of this study were to describe sagittal spinal alignment and pelvic tilt (LSA) in a slump sitting position and to explore the association of sagittal spine and pelvic tilt with back pain (BP) among 8–12-year-old children. It was a cross-sectional study. Sagittal spinal curvatures, BP and pelvic tilt were assessed in 582 students from 14 elementary schools. It was found that 53.44% of children had slight thoracic hyperkyphosis and that 48.80% presented moderate lumbar hyperkyphosis and 38.66% presented slight lumbar hyperkyphosis. Those who did not suffer from BP in any part of the back had a higher lumbar kyphosis (24.64 ± 7.84) or a greater LSA (107.27 ± 5.38) than children who had some type of BP in the previous year or week (lumbar kyphosis: 23.08 ± 8.06; LSA: 105.52 ± 6.00), although with no clinically relevant differences. In fact, neither sufferers nor those who did not have BP presented normal mean values for lumbar kyphosis or LSA according to normality references. This study demonstrates the need to assess sagittal morphotype in childhood since schoolchildren remain incorrectly seated for many hours and it greatly affects their spinal curvatures.
The Inventory of Quality in Early Intervention Centres for Service Providers: Preliminary Validating Study in a Spanish Sample
Authors: Jemes-Campaña Inmaculada-Concepción , Romero-Galisteo Rita-Pilar , Labajos-Manzanares María-Teresa , Gálvez-Ruiz Pablo , Moreno-Morales Noelia
Published: April 2020

Evaluating the service quality in early intervention (EI) from the perspective of professionals with knowledge in this area is essential for the improvement of EI centres. In this study, we aimed to test the reliability and validity of the adapted Inventory of Quality in Early Intervention Centres-P (IQEIC-P) in a sample of professionals who worked in EI centres. Three hundred and twenty-four professionals of 85 EI Spanish centres were recruited for this research. Various psychometric analyses were used to evaluate the factorial structure, the internal consistency, factorial validity and construct validity. A 5-dimension structure was obtained in the exploratory factor analysis (EFA). The results showed an adequate internal consistency (Cronbach’s alpha values between 0.71 and 0.83, and composite reliability (CR) values higher than 0.70), as well as satisfactory convergent and discriminant validity (average variance extracted (AVE) values above 0.50). In the confirmatory factor analysis, good model fit indicators were observed. The IQEIC-P showed adequate psychometric properties, demonstrating to be a valid instrument for the evaluation of service quality in EI centres from the perspective of professionals. The benefits will influence the professionals themselves, and they will have a positive and direct impact on the families that are attended to in these centres.
Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens
Authors: Yip Cyril Chik-Yan , Ho Chi-Chun , Chan Jasper Fuk-Woo , To Kelvin Kai-Wang , Chan Helen Shuk-Ying , Wong Sally Cheuk-Ying , Leung Kit-Hang , Fung Agnes Yim-Fong , Ng Anthony Chin-Ki , Zou Zijiao , Tam Anthony Raymond , Chung Tom Wai-Hin , Chan Kwok-Hung , Hung Ivan Fan-Ngai , Cheng Vincent Chi-Chung , Tsang Owen Tak-Yin , Tsui Stephen Kwok Wing , Yuen Kwok-Yung
Published: April 2020

The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths. In a containment-based strategy, rapid, sensitive and specific testing is important in epidemiological control and clinical management. Using 96 SARS-CoV-2 and 104 non-SARS-CoV-2 coronavirus genomes and our in-house program, GolayMetaMiner, four specific regions longer than 50 nucleotides in the SARS-CoV-2 genome were identified. Primers were designed to target the longest and previously untargeted nsp2 region and optimized as a probe-free real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. The new COVID-19-nsp2 assay had a limit of detection (LOD) of 1.8 TCID50/mL and did not amplify other human-pathogenic coronaviruses and respiratory viruses. Assay reproducibility in terms of cycle threshold (Cp) values was satisfactory, with the total imprecision (% CV) values well below 5%. Evaluation of the new assay using 59 clinical specimens from 14 confirmed cases showed 100% concordance with our previously developed COVID-19-RdRp/Hel reference assay. A rapid, sensitive, SARS-CoV-2-specific real-time RT-PCR assay, COVID-19-nsp2, was developed.
MiR-144: A New Possible Therapeutic Target and Diagnostic/Prognostic Tool in Cancers
Authors: Kooshkaki Omid , Rezaei Zohre , Rahmati Meysam , Vahedi Parviz , Derakhshani Afshin , Brunetti Oronzo , Baghbanzadeh Amir , Mansoori Behzad , Silvestris Nicola , Baradaran Behzad
Published: April 2020

MicroRNAs (miRNAs) are small and non-coding RNAs that display aberrant expression in the tissue and plasma of cancer patients when tested in comparison to healthy individuals. In past decades, research data proposed that miRNAs could be diagnostic and prognostic biomarkers in cancer patients. It has been confirmed that miRNAs can act either as oncogenes by silencing tumor inhibitors or as tumor suppressors by targeting oncoproteins. MiR-144s are located in the chromosomal region 17q11.2, which is subject to significant damage in many types of cancers. In this review, we assess the involvement of miR-144s in several cancer types by illustrating the possible target genes that are related to each cancer, and we also briefly describe the clinical applications of miR-144s as a diagnostic and prognostic tool in cancers.
Recent Advances in Psoriasis Research; the Clue to Mysterious Relation to Gut Microbiome
Authors: Komine Mayumi
Published: April 2020

Psoriasis is a chronic inflammatory cutaneous disease, characterized by activated plasmacytoid dendritic cells, myeloid dendritic cells, Th17 cells, and hyperproliferating keratinocytes. Recent studies revealed skin-resident cells have pivotal roles in developing psoriatic skin lesions. The balance in effector T cells and regulatory T cells is disturbed, leading Foxp3-positive regulatory T cells to produce proinflammatory IL-17. Not only acquired but also innate immunity is important in psoriasis pathogenesis, especially in triggering the disease. Group 3 innate lymphoid cell are considered one of IL-17-producing cells in psoriasis. Short chain fatty acids produced by gut microbiota stabilize expression of Foxp3 in regulatory T cells, thereby stabilizing their function. The composition of gut microbiota influences the systemic inflammatory status, and associations been shown with diabetes mellitus, cardiovascular diseases, psychomotor diseases, and other systemic inflammatory disorders. Psoriasis has been shown to frequently comorbid with diabetes mellitus, cardiovascular diseases, psychomotor disease and obesity, and recent report suggested the similar abnormality in gut microbiota as the above comorbid diseases. However, the precise mechanism and relation between psoriasis pathogenesis and gut microbiota needs further investigation. This review introduces the recent advances in psoriasis research and tries to provide clues to solve the mysterious relation of psoriasis and gut microbiota.
Interferon Response in Hepatitis C Virus-Infected Hepatocytes: Issues to Consider in the Era of Direct-Acting Antivirals
Authors: Sung Pil Soo , Shin Eui-Cheol
Published: April 2020

When interferons (IFNs) bind to their receptors, they upregulate numerous IFN-stimulated genes (ISGs) with antiviral and immune regulatory activities. Hepatitis C virus (HCV) is a single-stranded, positive-sense RNA virus that affects over 71 million people in the global population. Hepatocytes infected with HCV produce types I and III IFNs. These endogenous IFNs upregulate a set of ISGs that negatively impact the outcome of pegylated IFN-α and ribavirin treatments, which were previously used to treat HCV. In addition, the IFNL4 genotype was the primary polymorphism responsible for a suboptimal treatment response to pegylated IFN-α and ribavirin. However, recently developed direct-acting antivirals have demonstrated a high rate of sustained virological response without pegylated IFN-α. Herein, we review recent studies on types I and III IFN responses in HCV-infected hepatocytes. In particular, we focused on open issues related to IFN responses in the direct-acting antiviral era.
Structural Characterization of an ACP from Thermotoga maritima: Insights into Hyperthermal Adaptation
Authors: Lee Yeongjoon , Jang Ahjin , Jeong Min-Cheol , Park Nuri , Park Jungwoo , Lee Woo Cheol , Cheong Chaejoon , Kim Yangmee
Published: April 2020

Thermotoga maritima, a deep-branching hyperthermophilic bacterium, expresses an extraordinarily stable Thermotoga maritima acyl carrier protein (Tm-ACP) that functions as a carrier in the fatty acid synthesis system at near-boiling aqueous environments. Here, to understand the hyperthermal adaptation of Tm-ACP, we investigated the structure and dynamics of Tm-ACP by nuclear magnetic resonance (NMR) spectroscopy. The melting temperature of Tm-ACP (101.4 °C) far exceeds that of other ACPs, owing to extensive ionic interactions and tight hydrophobic packing. The D59 residue, which replaces Pro/Ser of other ACPs, mediates ionic clustering between helices III and IV. This creates a wide pocket entrance to facilitate the accommodation of long acyl chains required for hyperthermal adaptation of the T. maritima cell membrane. Tm-ACP is revealed to be the first ACP that harbor an amide proton hyperprotected against hydrogen/deuterium exchange for I15. The hydrophobic interactions mediated by I15 appear to be the key driving forces of the global folding process of Tm-ACP. Our findings provide insights into the structural basis of the hyperthermal adaptation of ACP, which might have allowed T. maritima to survive in hot ancient oceans.
Bioactive Characterization of Packaging Foils Coated by Chitosan and Polyphenol Colloidal Formulations
Authors: Potrč Sanja , Sterniša Meta , Smole Možina Sonja , Knez Hrnčič Maša , Fras Zemljič Lidija
Published: April 2020

Polypropylene (PP) and polyethylene (PE) foils, previously activated by ultraviolet (UV)/ozone, were functionalized using chitosan-extract nanoparticle dispersions. A solution of macromolecular chitosan was applied onto foils as a first layer, followed by the deposition of various extracts encapsulated into chitosan nanoparticles, which were attached as an upper layer. Functionalized foils were analyzed from a bioactive point of view, i.e., regarding antimicrobial and antioxidant activity. Desorption kinetics were also studied. Moreover, barrier properties were examined, as the most important parameter influencing antimicrobial and antioxidant activity. Finally, all these properties were correlated with different surface parameters, determined previously, in order to understand if there is any direct correlation between surface elemental composition, surface charge, contact angle, or morphology and a specific bioactive property. It was shown that great bioactive properties were introduced due to the additive effect of antimicrobial chitosan and antioxidative plant extracts. Moreover, oxygen permeability decreased significantly, and the migration of polyphenols and chitosan from the foil surface was below the OML (overall migration limit), which is very important for food industry applications. Furthermore, surface properties of foils influence to some extent the desired bioactivity.
Trace Elements, PPARs, and Metabolic Syndrome
Authors: Shi Yujie , Zou Yixin , Shen Ziyue , Xiong Yonghong , Zhang Wenxiang , Liu Chang , Chen Siyu
Published: April 2020

Metabolic syndrome (MetS) is a constellation of metabolic derangements, including central obesity, insulin resistance, hypertension, glucose intolerance, and dyslipidemia. The pathogenesis of MetS has been intensively studied, and now many factors are recognized to contribute to the development of MetS. Among these, trace elements influence the structure of proteins, enzymes, and complex carbohydrates, and thus an imbalance in trace elements is an independent risk factor for MetS. The molecular link between trace elements and metabolic homeostasis has been established, and peroxisome proliferator-activated receptors (PPARs) have appeared as key regulators bridging these two elements. This is because on one hand, PPARs are actively involved in various metabolic processes, such as abdominal adiposity and insulin sensitivity, and on the other hand, PPARs sensitively respond to changes in trace elements. For example, an iron overload attenuates hepatic mRNA expression of Ppar-α; zinc supplementation is considered to recover the DNA-binding activity of PPAR-α, which is impaired in steatotic mouse liver; selenium administration downregulates mRNA expression of Ppar-γ, thereby improving lipid metabolism and oxidative status in the liver of high-fat diet (HFD)-fed mice. More importantly, PPARs’ expression and activity are under the control of the circadian clock and show a robust 24 h rhythmicity, which might be the reasons for the side effects and the clinical limitations of trace elements targeting PPARs. Taken together, understanding the casual relationships among trace elements, PPARs’ actions, and the pathogenesis of MetS is of great importance. Further studies are required to explore the chronopharmacological effects of trace elements on the diurnal oscillation of PPARs and the consequent development of MetS.
Simulation and Modeling of Telocytes Behavior in Signaling and Intercellular Communication Processes
Authors: Cretoiu Dragos , Roatesi Simona , Bica Ion , Plesca Cezar , Stefan Amado , Bajenaru Oana , Condrat Carmen Elena , Cretoiu Sanda Maria
Published: April 2020

Background: Telocytes (TCs) are unique interstitial or stromal cells of mesodermal origin, defined by long cellular extensions called telopodes (Tps) which form a network, connecting them to surrounding cells. TCs were previously found around stem and progenitor cells, and were thought to be most likely involved in local tissue metabolic equilibrium and regeneration. The roles of telocytes are still under scientific scrutiny, with existing studies suggesting they possess various functions depending on their location. Methods: Human myometrium biopsies were collected from pregnant and non-pregnant women, telocytes were then investigated in myometrial interstitial cell cultures based on morphological criteria and later prepared for time-lapse microscopy. Semi-analytical and numerical solutions were developed to highlight the geometric characteristics and the behavior of telocytes. Results: Results were gathered in a database which would further allow efficient telocyte tracking and indexing in a content-based image retrieval (CBIR) of digital medical images. Mathematical analysis revealed pivotal information regarding the homogeneity, hardness and resistance of telocytes’ structure. Cellular activity models were monitored in vitro, therefore supporting the creation of databases of telocyte images. Conclusions: The obtained images were analyzed, using segmentation techniques and mathematical models in conjunction with computer simulation, in order to depict TCs behavior in relation to surrounding cells. This paper brings an important contribution to the development of bioinformatics systems by creating software-based telocyte models that could be used both for diagnostic and educational purposes.
The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines
Authors: Kazmierczak Dominika , Jopek Karol , Sterzynska Karolina , Ginter-Matuszewska Barbara , Nowicki Michal , Rucinski Marcin , Januchowski Radoslaw
Published: April 2020

Ovarian cancer rates the highest mortality among all gynecological malignancies. The main reason for high mortality is the development of drug resistance. It can be related to increased expression of drug transporters and increased expression of extracellular matrix (ECM) proteins. Our foremost aim was to exhibit alterations in the miRNA expression levels in cisplatin (CIS), paclitaxel (PAC), doxorubicin (DOX), and topotecan (TOP)—resistant variants of the W1 sensitive ovarian cancer cell line—using miRNA microarray. The second goal was to identify miRNAs responsible for the regulation of drug-resistant genes. According to our observation, alterations in the expression of 40 miRNAs were present. We could observe that, in at least one drug-resistant cell line, the expression of 21 miRNAs was upregulated and that of 19 miRNAs was downregulated. We identified target genes for 22 miRNAs. Target analysis showed that miRNA regulates key genes responsible for drug resistance. Among others, we observed regulation of the ATP-binding cassette subfamily B member 1 gene (ABCB1) in the paclitaxel-resistant cell line by miR-363 and regulation of the collagen type III alpha 1 chain gene (COL3A1) in the topotekan-resistant cell line by miR-29a.
Adiponectin and Its Mimics on Skeletal Muscle: Insulin Sensitizers, Fat Burners, Exercise Mimickers, Muscling Pills … or Everything Together?
Authors: Abou-Samra Michel , Selvais Camille M. , Dubuisson Nicolas , Brichard Sonia M.
Published: April 2020

Adiponectin (ApN) is a hormone abundantly secreted by adipocytes and it is known to be tightly linked to the metabolic syndrome. It promotes insulin-sensitizing, fat-burning, and anti-atherosclerotic actions, thereby effectively counteracting several metabolic disorders, including type 2 diabetes, obesity, and cardiovascular diseases. ApN is also known today to possess powerful anti-inflammatory/oxidative and pro-myogenic effects on skeletal muscles exposed to acute or chronic inflammation and injury, mainly through AdipoR1 (ApN specific muscle receptor) and AMP-activated protein kinase (AMPK) pathway, but also via T-cadherin. In this review, we will report all the beneficial and protective properties that ApN can exert, specifically on the skeletal muscle as a target tissue. We will highlight its effects and mechanisms of action, first in healthy skeletal muscle including exercised muscle, and second in diseased muscle from a variety of pathological conditions. In the end, we will go over some of AdipoRs agonists that can be easily produced and administered, and which can greatly mimic ApN. These interesting and newly identified molecules could pave the way towards future therapeutic approaches to potentially prevent or combat not only skeletal muscle disorders but also a plethora of other diseases with sterile inflammation or metabolic dysfunction.
Aryl-4,5-dihydro-1H-pyrazole-1-carboxamide Derivatives Bearing a Sulfonamide Moiety Show Single-digit Nanomolar-to-Subnanomolar Inhibition Constants against the Tumor-associated Human Carbonic Anhydrases IX and XII
Authors: Hargunani Priya , Tadge Nikhil , Ceruso Mariangela , Leitans Janis , Kazaks Andris , Tars Kaspars , Gratteri Paola , Supuran Claudiu T. , Nocentini Alessio , Toraskar Mrunmayee P.
Published: April 2020

A series of new 3-phenyl-5-aryl-N-(4-sulfamoylphenyl)-4,5-dihydro-1H-pyrazole-1-carboxamide derivatives was designed here, synthesized, and studied for carbonic anhydrase (CAs, EC 4.2.1.1) inhibitory activity against the human (h) isozymes I, II, and VII (cytosolic, off-target isoforms), and IX and XII (anticancer drug targets). Generally, CA I was not effectively inhibited, whereas effective inhibitors were identified against both CAs II (KIs in the range of 5.2–233 nM) and VII (KIs in the range of 2.3–350 nM). Nonetheless, CAs IX and XII were the most susceptible isoforms to this class of inhibitors. In particular, compounds bearing an unsubstituted phenyl ring at the pyrazoline 3 position showed 1.3–1.5 nM KIs against CA IX. In contrast, a subset of derivatives having a 4-halo-phenyl at the same position of the aromatic scaffold even reached subnanomolar KIs against CA XII (0.62–0.99 nM). Docking studies with CA IX and XII were used to shed light on the derivative binding mode driving the preferential inhibition of the tumor-associated CAs. The identified potent and selective CA IX/XII inhibitors are of interest as leads for the development of new anticancer strategies.
mPTP Proteins Regulated by Streptozotocin-Induced Diabetes Mellitus Are Effectively Involved in the Processes of Maintaining Myocardial Metabolic Adaptation
Authors: Andelova Natalia , Waczulikova Iveta , Talian Ivan , Sykora Matus , Ferko Miroslav
Published: April 2020

Mitochondrial permeability transition pores (mPTPs) have become an important topic in investigating the initiation and signaling pathways involved in cardioprotection. Experimental streptozotocin-induced diabetes mellitus (D) was shown to provide sufficient protection to the myocardium via compensatory mechanisms enabling mitochondria to produce energy under pathological conditions during the acute phase. The hypothesized involvement of mPTPs in these processes prompted us to use liquid chromatography and mass spectrometry-based proteomic analysis to investigate the effects of the acute-phase D condition on the structural and regulatory components of this multienzyme complex and the changes caused by compensation events. We detected ADT1, ATP5H, ATPA, and ATPB as the most abundant mPTP proteins. The between-group differences in protein abundance of the mPTP complex as a whole were significantly upregulated in the D group when compared with the control (C) group (p = 0.0106), but fold changes in individual protein expression levels were not significantly altered except for ATP5H, ATP5J, and KCRS. However, none of them passed the criterion of a 1.5-fold change in differential expression for biologically meaningful change. Visualization of the (dis-)similarity between the C and D groups and pairwise correlations revealed different patterns of protein interactions under the C and D conditions which may be linked to endogenous protective processes, of which beneficial effects on myocardial function were previously confirmed.
Efficacy of Immune Checkpoint Inhibitor Monotherapy for Advanced Non-Small-Cell Lung Cancer with ALK Rearrangement
Authors: Oya Yuko , Kuroda Hiroaki , Nakada Takeo , Takahashi Yusuke , Sakakura Noriaki , Hida Toyoaki
Published: April 2020

Programmed death-ligand 1 (PD-L1) expression is a predictor of immune checkpoint inhibitor (ICI) treatment efficacy. The clinical efficacy of ICIs for non-small-cell lung cancer (NSCLC) patients harboring major mutations, such as EGFR or ALK mutations, is limited. We genotyped 190 patients with advanced lung adenocarcinomas who received nivolumab or pembrolizumab monotherapy, and examined the efficacy in NSCLC patients with or without major mutations. Among the patients enrolled in the genotyping study, 47 patients harbored EGFR mutations, 25 patients had KRAS mutations, 5 patients had a HER2 mutation, 6 patients had a BRAF mutation, and 7 patients had ALK rearrangement. The status of PD-L1 expression was evaluated in 151 patients, and the rate of high PD-L1 expression (≥50%) was significantly higher in patients with ALK mutations. The progression-free survival was 0.6 (95% CI: 0.2–2.1) months for ALK-positive patients and 1.8 (95% CI: 1.2–2.1) months for EGFR-positive patients. All patients with ALK rearrangement showed disease progression within three months from the initiation of anti-PD-1 treatment. Our data suggested that ICI treatment was significantly less efficacious in patients with ALK rearrangement than in patients with EGFR mutations, and PD-L1 expression was not a critical biomarker for ICI treatment for patients with one of these mutations.
Directing Stem Cell Commitment by Amorphous Calcium Phosphate Nanoparticles Incorporated in PLGA: Relevance of the Free Calcium Ion Concentration
Authors: Gröninger Olivier , Hess Samuel , Mohn Dirk , Schneider Elia , Stark Wendelin , Märsmann Sonja , Wolint Petra , Calcagni Maurizio , Cinelli Paolo , Buschmann Johanna
Published: April 2020

The microenvironment of mesenchymal stem cells (MSCs) is responsible for the modulation in MSC commitment. Nanocomposites with an inorganic and an organic component have been investigated, and osteogenesis of MSCs has been attributed to inorganic phases such as calcium phosphate under several conditions. Here, electrospun meshes and two-dimensional films of poly(lactic-co-glycolic acid) (PLGA) or nanocomposites of PLGA and amorphous calcium phosphate nanoparticles (PLGA/aCaP) seeded with human adipose-derived stem cells (ASCs) were analyzed for the expression of selected marker genes. In a two-week in vitro experiment, osteogenic commitment was not found to be favored on PLGA/aCaP compared to pure PLGA. Analysis of the medium revealed a significant reduction of the Ca2+ concentration when incubated with PLGA/aCaP, caused by chemical precipitation of hydroxyapatite (HAp) on aCaP seeds of PLGA/aCaP. Upon offering a constant Ca2+ concentration, however, the previously observed anti-osteogenic effect was reversed: alkaline phosphatase, an early osteogenic marker gene, was upregulated on PLGA/aCaP compared to pristine PLGA. Hence, in addition to the cell–material interaction, the material–medium interaction was also important for the stem cell commitment here, affecting the cell–medium interaction. Complex in vitro models should therefore consider all factors, as coupled impacts might emerge.
Detection of the Lychee Erinose Mite, Aceria litchii (Keifer) (Acari: Eriophyidae) in Florida, USA: A Comparison with Other Alien Populations
Authors: Carrillo Daniel , Cruz Luisa F. , Revynthi Alexandra M. , Duncan Rita E. , Bauchan Gary R. , Ochoa Ronald , Kendra Paul E. , Bolton Samuel J.
Published: April 2020

The lychee erinose mite (LEM), Aceria litchii (Keifer) is a serious pest of lychee (Litchi chinensis Sonn.). LEM causes a type of gall called ‘erineum’ (abnormal felty growth of trichomes from the epidermis), where it feeds, reproduces and protects itself from biotic and abiotic adversities. In February of 2018, LEM was found in a commercial lychee orchard on Pine Island, Florida. Infestations were recorded on young leaves, stems, and inflorescences of approximately 30 young trees (1.5–3.0 yrs.) of three lychee varieties presenting abundant new growth. Although LEM is present in Hawaii, this mite is a prioritized quarantine pest in the continental USA and its territories. Florida LEM specimens showed small morphological differences from the original taxonomic descriptions of Keifer (1943) and Huang (2008). The observed differences are probably an artifact of the drawings in the original descriptions. Molecular comparisons were conducted on the DNA of LEM specimens from India, Hawaii, Brazil, Taiwan, Australia and Florida. The amplified COI fragment showed very low nucleotide variation among the locations and thus, could be used for accurate LEM identification. The ITS1 sequences and partial 5.8S fragments displayed no nucleotide differences for specimens from any of the locations except Australia. Consistent differences were observed in the ITS2 and 28S fragments. The ITS1-ITS2 concatenated phylogeny yielded two lineages, with Australia in one group and Hawaii, India, Brazil, Florida and Taiwan in another. Specimens from Taiwan and Florida present identical ITS and rDNA segments, suggesting a common origin; however, analysis of additional sequences is needed to confirm the origin of the Florida population.
Open Data for Open Questions in Comparative Nutrition
Authors: Morimoto Juliano , Lihoreau Mathieu
Published: April 2020

Achieving a better understanding of the consequences of nutrition to animal fitness and human health is a major challenge of our century. Nutritional ecology studies increasingly use nutritional landscapes to map the complex interacting effects of nutrient intake on animal performances, in a wide range of species and ecological contexts. Here, we argue that opening access to these hard-to-obtain, yet considerably insightful, data is fundamental to develop a comparative framework for nutrition research and offer new quantitative means to address open questions about the ecology and evolution of nutritional processes.