bluuebunny/crossref_metadata_2025 huggingface dataset:
Health Outcomes Associated with Adherence to Antidepressant Use during Acute and Continuation Phases of Depression Treatment among Older Adults with Dementia and Major Depressive Disorder
Authors: Bhattacharjee Sandipan , Naeem Suniya , Knapp Shannon M. , Lee Jeannie K. , Patanwala Asad E. , Vadiei Nina , Malone Daniel C. , Lo-Ciganic Wei-Hsuan , Burke William J
Published: October 2020

Objectives: To examine health outcomes associated with adherence to Healthcare Effectiveness Data and Information Set (HEDIS) antidepressant medication management (AMM) during acute and continuation phases of depression treatment among older adults with dementia and major depressive disorder (MDD). Design: Retrospective cohort study. Setting: Medicare 5% sample data (2011–2013). Participants: Older adults (aged 65 years or older) with dementia and MDD. Measurements: The first antidepressant prescription claim from 1 May 2011 through 30 April 2012 was considered the index prescription start date (IPSD). Adherence during acute- and continuation-phase AMM was based on HEDIS guidelines. Study outcomes included all-cause mortality, all-cause hospitalization, and falls/factures (with mortality being the competing event for hospitalization and falls/fractures) during follow-up from end of acute-/continuation-phase AMM adherence. Due to the proportionality assumption violation of Cox models, fully non-parametric approaches (Kaplan–Meier and modified Gray’s test) were used for time-to-event analysis adjusting for the inverse probability of treatment weights. Results: Final study samples consisted of 4330 (adherent (N) = 3114 (71.92%)) and 3941 (adherent (N) = 2407 (61.08%)) older adults with dementia and MDD during acute- and continuation-phase treatments, respectively. No significant difference (p > 0.05) between adherent and non-adherent groups was observed for all-cause mortality and falls/fractures in both the acute and continuation phases. There was a significant difference in time to all-cause hospitalization during acute-phase treatment (p = 0.018), with median times of 530 (95% CI: 499–587) and 425 (95% CI: 364–492) days for adherent and non-adherent groups, respectively. Conclusions: Acute-phase adherence to HEDIS AMM was associated with reductions in all-cause hospitalization risk among older adults with dementia and MDD.
Clinical Usefulness of New R-R Interval Analysis Using the Wearable Heart Rate Sensor WHS-1 to Identify Obstructive Sleep Apnea: OSA and RRI Analysis Using a Wearable Heartbeat Sensor
Authors: Arikawa Takuo , Nakajima Toshiaki , Yazawa Hiroko , Kaneda Hiroyuki , Haruyama Akiko , Obi Syotaro , Amano Hirohisa , Sakuma Masashi , Toyoda Shigeru , Abe Shichiro , Tsutsumi Takeshi , Matsui Taishi , Nakata Akio , Shinozaki Ryo , Miyamoto Masayuki , Inoue Teruo
Published: October 2020

Obstructive sleep apnea (OSA) is highly associated with cardiovascular diseases, but most patients remain undiagnosed. Cyclic variation of heart rate (CVHR) occurs during the night, and R-R interval (RRI) analysis using a Holter electrocardiogram has been reported to be useful in screening for OSA. We investigated the usefulness of RRI analysis to identify OSA using the wearable heart rate sensor WHS-1 and newly developed algorithm. WHS-1 and polysomnography simultaneously applied to 30 cases of OSA. By using the RRI averages calculated for each time series, tachycardia with CVHR was identified. The ratio of integrated RRIs determined by integrated RRIs during CVHR and over all sleep time were calculated by our newly developed method. The patient was diagnosed as OSA according to the predetermined criteria. It correlated with the apnea hypopnea index and 3% oxygen desaturation index. In the multivariate analysis, it was extracted as a factor defining the apnea hypopnea index (r = 0.663, p = 0.003) and 3% oxygen saturation index (r = 0.637, p = 0.008). Twenty-five patients could be identified as OSA. We developed the RRI analysis using the wearable heart rate sensor WHS-1 and a new algorithm, which may become an expeditious and cost-effective screening tool for identifying OSA.
Association between Carotid Artery Calcification and Periodontal Disease Progression in Japanese Men and Women: A Cross-Sectional Study
Authors: Dewake Nanae , Ishioka Yasuaki , Uchida Keiichi , Taguchi Akira , Higashi Yukihito , Yoshida Akihiro , Yoshinari Nobuo
Published: October 2020

Objective: To evaluate the association between alveolar bone loss (ABL) detected on panoramic radiographs and carotid artery calcification (CAC) detected on computed tomography (CT). Methods: The study subjects included 295 patients (mean age ± SD: 64.6 ± 11.8 years) who visited the Matsumoto Dental University Hospital. The rate of ABL and the number of present teeth were measured on panoramic radiographs. Univariate analyses with t-tests and chi-squared tests were performed to evaluate the differences in age, gender, history of diseases, number of present teeth, and the ABL between subjects, with and without CAC. Moreover, multivariate logistic regression analysis, with forward selection and receiver operating characteristic curve (ROC) analysis, was performed. Results: The number of subjects without and with CAC was 174 and 121, respectively. Univariate analyses revealed that CAC was significantly associated with age, hypertension, osteoporosis, number of present teeth, and ABL. Multivariate logistic regression analysis adjusted for covariates revealed that the presence of CAC was significantly associated with ABL (OR = 1.233, 95% CI = 1.167–1.303). In the ROC analysis for predicting the presence of CAC, the the area under the ROC curve was the highest at 0.932 (95% CI = 0.904–0.960) for ABL, which was significant. Conclusions: Our results suggest that the measurement of ABL on panoramic radiographs may be an effective approach to identifying patients with an increased risk of CAC.
SPH Simulations of Real Sea Waves Impacting a Large-Scale Structure
Authors: Altomare Corrado , Tafuni Angelantonio , Domínguez José M. , Crespo Alejandro J. C. , Gironella Xavi , Sospedra Joaquim
Published: October 2020

The Pont del Petroli is a dismissed pier in the area of Badalona, Spain, with high historical and social value. This structure was heavily damaged in January 2020 during the storm Gloria that hit southeastern Spain with remarkable strength. The reconstruction of the pier requires the assessment and characterization of the wave loading that determined the structural failure. Therefore, a state-of-the-art Computational Fluid Dynamic (CFD) code was employed herein as an aid for a planned experimental campaign that will be carried out at the Maritime Engineering Laboratory of Universitat Politècnica de Catalunya-BarcelonaTech (LIM/UPC). The numerical model is based on Smoothed Particle Hydrodynamics (SPH) and has been employed to simulate conditions very similar to those that manifested during the storm Gloria. The high computational cost for a full 3-D simulation has been alleviated by means of inlet boundary conditions, allowing wave generation very close to the structure. Numerical results reveal forces higher than the design loads of the pier, including both self-weight and accidental loads. This demonstrates that the main failure mechanism that led to severe structural damage of the pier during the storm is related to the exceeded lateral soil resistance. To the best of the authors’ knowledge, this research represents the first known application of SPH open boundary conditions to model a real-world engineering case.
Derivation of Engineering Design Criteria for Flow Field Around Intake Structure: A Numerical Simulation Study
Authors: Chie Lee Hooi , Abd Wahab Ahmad Khairi
Published: October 2020

The primary environmental impact caused by seawater intake operation is marine life impingement resulting from the intake velocity. Environmental Protection Agency (EPA) of United State has regulated the use of velocity cap fitted at intake structures to reduce the marine life impingement. The engineering design parameters of velocity cap has not been well explored to date. This study has been set to determine the fundamental relationships between intake velocity and design parameters of velocity cap, using computational fluid dynamic (CFD) model. A set of engineering design criteria for velocity cap design are derived. The numerical evidence yielded in this study show that the velocity cap should be designed with vertical opening (Hvc) and horizontal shelf (ℓvc). The recommended intake opening ratio (Or) shall be 0.36 Vr−0.31, where Or = Hvc/ℓvc and Vr =V0/Vpipe. Vo is the velocity at the intake window and Vpipe is the suction velocity at the intake pipe. The volume ratio (ωr) between the velocity cap (ωvc) and intake tower (ωIT) is recommended at 0.11 Vr−1.23. The positive outlooks that yielded from this study can be served as a design reference for velocity cap to mitigate the detrimental impacts from the existing intake structure.
Novel 4,6-Disubstituted s-Triazin-2-yl Amino Acid Derivatives as Promising Antifungal Agents
Authors: Abd Alhameed Rakia , Almarhoon Zainab , N. Sholkamy Essam , Ali Khan Salman , Ul-Haq Zaheer , Sharma Anamika , G. de la Torre Beatriz , Albericio Fernando , El-Faham Ayman
Published: October 2020

A novel series of 4,6-disubstituted s-triazin-2-yl amino acid derivatives was prepared and characterized. Most of them showed antifungal activity against Candida albicans compared to clotrimazole (standard drug). Compounds bearing aniline derivatives, piperidine and glycine on the triazine core showed the highest inhibition zones at concentrations of 50, 100, 200, and 300 μg per disc. In addition, docking studies revealed that all the compounds accommodated well in the active site residues of N-myristoltransferase (NMT) and exhibited complementarity, which explains the observed antifungal activity. Interestingly, none of these compounds showed antibacterial activity.
Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts
Authors: Calvo Enrique , Corbacho-Alonso Nerea , Sastre-Oliva Tamara , Nuñez Estefania , Baena-Galan Patricia , Hernandez-Fernandez German , Rodriguez-Cola Miguel , Jimenez-Velasco Irena , Corrales Fernando J. , Gambarrutta-Malfati Claudia , Gutierrez-Henares Francisco , Lopez-Dolado Elisa , Gil-Agudo Angel , Vazquez Jesus , Mourino-Alvarez Laura , Barderas Maria G.
Published: October 2020

The COVID-19 pandemic represents an unprecedented global challenge in this century. COVID-19 is a viral respiratory infection, yet the clinical characteristics of this infection differ in spinal cord injury patients from those observed in the general population. Cough and asthenia are the most frequent symptoms in this population. Moreover, infected spinal cord injury patients rarely present complications that require admission to an Intensive Care Unit, in contrast to the general population. Thus, there is a clear need to understand how COVID-19 affects spinal cord injury patients from a molecular perspective. Here, we employed an -omics strategy in order to identify variations in protein abundance in spinal cord injury patients with and without COVID-19. After a quantitative differential analysis using isobaric tags and mass spectrometry and a verification phase, we have found differences mainly related to coagulation and platelet activation. Our results suggest a key role of heparin in the response of spinal cord injury patients to COVID-19 infection, showing a significant correlation between these proteins and heparin dose. Although the number of patients is limited, these data may shed light on new therapeutic options to improve the management these patients and, possibly, those of the general population as well.
Gender Differences in Genetic Associations of RAB38 with Urinary Protein-to-Creatinine Ratio (UPCR) Levels in Diabetic Nephropathy Patients
Authors: Yu Zhi-Lei , Wong Chung-Shun , Lai Yi Ting , Chou Wan-Hsuan , Faridah Imaniar Noor , Kao Chih-Chin , Lin Yuh-Feng , Chang Wei-Chiao
Published: October 2020

Renal dysfunction is common in patients with diabetes mellitus (DM). Previous findings from a meta-analysis of GWAS indicated that the variation of RAB38/CTSC is highly associated with the urinary albumin-to-creatinine ratio (UACR) in European populations. In addition, RAB38 knockout rats showed an increase in urinary albumins. Although the prevalence of chronic kidney disease is high in Taiwan, the role of genetic variants in diabetic renal function is still unclear. In the current study, 275 diabetic nephropathy (DN) patients were recruited to perform a genetic association study. Our results indicated that rs1027027, rs302647, and rs302646 in RAB38 were significantly associated with urinary protein-to-creatinine ratio (UPCR) levels in DN patients. Importantly, after analysis stratified by gender, a significant genetic influence on UPCR levels was observed in the male population. The findings confirmed the roles of gender and variants of RAB38 in the risk of UPCR in Diabetic Nephropathy patients.
Spatial Variation in Environmental Impacts of Sugarcane Expansion in Brazil
Authors: Vera Ivan , Wicke Birka , Hilst Floor van der
Published: October 2020

In the past decades, sugarcane production in Brazil has expanded rapidly to meet increasing ethanol demand. The large majority of this expansion occurred in Sao Paulo state. We used an integrated approach considering location-specific biophysical characteristics to determine the environmental impacts of sugarcane expansion and their spatial variation in Sao Paulo state (2004–2015). The included environmental impacts are greenhouse gas (GHG) emissions, biodiversity, soil erosion, and water quantity. All impacts were integrated into a single environmental performance index to determine trade-offs between impacts. Our results show a strong spatial variation in environmental impacts and trade-offs between them. The magnitude and direction of these impacts are mostly driven by the type of land use change and by the heterogeneity of the biophysical conditions. Areas where expansion of sugar cane has resulted in mostly negative environmental impacts are located in the center and east of the state (related to the change of shrublands, eucalyptus, and forest), while areas where sugar cane expansion has resulted in positive impacts are located in the center-west and north (related to the change of annual crops). Identifying areas with mainly positive and negative impacts enables the development of strategies to mitigate negative effects and enhance positive ones for future sugarcane expansion.
(7E)-3-(4-Methoxyphenyl)-7-[(4-methoxyphenyl)methylidene]-4,5,6,7-tetrahydro-3aH-indazole
Authors: Hariyanti Hariyanti , Yanuar Arry , Kusmardi Kusmardi , Hayun Hayun
Published: October 2020

Indazole derivatives are well known to have various pharmacological activities. We synthesized a novel derivative of indazole, namely (7E)-3-(4-methoxyphenyl)-7-[(4-methoxyphenyl)methylidene]-4,5,6,7-tetrahydro-3aH-indazole by condensation reaction between 3-(4-methoxyphenyl)-3,3a,4,5,6,7-hexahydro-2H-indazole and 4-methoxy-benzaldehyde in good yield (61%).
Evaluation of the Milling Accuracy of Zirconia-Reinforced Lithium Silicate Crowns Fabricated Using the Dental Medical Device System: A Three-Dimensional Analysis
Authors: Kang Seen-Young , Yu Ji-Min , Lee Jun-Seok , Park Ki-Sook , Lee Seung-Youl
Published: October 2020

This study aimed to analyze the milling accuracy of lithium disilicate and zirconia-reinforced silicate crown fabricated using chairside computer-aided design/manufacturing (CAD/CAM) system. Mandibular left first premolar was selected for abutment. A master model was obtained for digital impression using an intraoral scanner, and crowns were designed using a CAD software design program. Amber Mill (AM), IPS e max CAD (IPS), and CELTRA DUO (CEL) were used in the CAD/CAM system, and a total 45 crowns (15 crowns each for AM, IPS, and CEL) was fabricated. Milling accuracy was analyzed with respect to trueness, measured by superimposing CAD design data and scan data through a three-dimensional program to compare the outer and inner surfaces and internal and external parts, thereby acquiring both quantitative and qualitative data. Data were analyzed using the non-parametric test and Kruskal–Wallis H test. In addition, the Mann–Whitney U test was used by applying the level of significance (0.05/3 = 0.016) adjusted by post-analysis Bonferroni correction. All the measured parts of the lithium disilicate and zirconia-reinforced silicate crowns showed statistically significant differences (p < 0.05). The lithium disilicate (AM and IPS) materials showed superior milling accuracy than the zirconia-reinforced lithium silicate (CEL) materials.
Preliminary Study on the Capability of the Novel Near Solidus Forming (NSF) Technology to Manufacture Complex Steel Components
Authors: Plata Gorka , Lozares Jokin , Sánchez Andrea , Hurtado Iñaki , Slater Carl
Published: October 2020

The benefits of the novel Near Solidus Forming (NSF) process has shown previously in its ability to produce steel components with comparable as-forged mechanical properties but with a cost reduction of 10–15%. This study further pushes the NSF technology to produce parts that are conventionally difficult to produce via conventional methods. A 2.7 kg 42CrMo4 steel grade component was manufactured into a complex geometry using only a 400t press. Different manufacturing parameters were evaluated to show their influence on the process and final component. A combination of X-ray fluorescence (XRF), optical microscopy and SEM analysis of the microstructure was also conducted revealing the deformation pattern of the material and shedding some light on how the material evolves during the process. The successful forging of these components shows the capability to produce previously deemed difficult geometries, with much a lower specification forging press, in a single deformation.
Effect of Yttrium and Rhenium Ion Implantation on the Performance of Nitride Ceramic Cutting Tools
Authors: Morozow Dmitrij , Siemiątkowski Zbigniew , Gevorkyan Edwin , Rucki Mirosław , Matijošius Jonas , Kilikevičius Artūras , Caban Jacek , Krzysiak Zbigniew
Published: October 2020

In the paper, the results of experimental investigations of ion implanted cutting tools performance are presented. The tools, made out of Si3N4 with additives typically used for turning of Ti-6Al-4V alloy, underwent implantation with ions of yttrium (Y+) and rhenium (Re+) using the metal vapor vacuum arc method. Distribution of ions on the tool surface was measured. The cutting tools were tested in turning process with measurement of cutting forces and analysis of wear. A rather unexpected result was the increased wear of the tool after Y+ implantation with 1 × 1017 ion/cm2. It was demonstrated, however, that the tool after Y+ 2 × 1017 ion/cm2 ion implantation provided the best machining performance.
Coupling Finite Element Analysis and the Theory of Critical Distances to Estimate Critical Loads in Al6060-T66 Tubular Beams Containing Notches
Authors: Sánchez Marcos , Cicero Sergio , Arroyo Borja , Álvarez José Alberto
Published: October 2020

This paper validates a methodology for the estimation of critical loads in tubular beams containing notch-type defects. The methodology is particularized for the case of Al6060-T66 tubular cantilever beams containing U-shaped notches. It consists in obtaining the stress field at the notch tip using finite element analysis (FEA) and the subsequent application of the theory of critical distances (TCD) to derive the corresponding critical load (or load-bearing capacity). The results demonstrate that this methodology provides satisfactory predictions of fracture loads.
Optimization of Open Die Ironing Process through Artificial Neural Network for Rapid Process Simulation
Authors: Mancini Silvia , Langellotto Luigi , Zangari Giovanni , Maccaglia Riccardo , Schino Andrea Di
Published: October 2020

The open die forging sequence design and optimization are usually performed by simulating many different configurations corresponding to different forging strategies. Finite element analysis (FEM) is a tool able to simulate the open die forging process. However, FEM is relatively slow and therefore it is not suitable for the rapid design of online forging processes. A new approach is proposed in this work in order to describe the plastic strain at the core of the piece. FEM takes into account the plastic deformation at the core of the forged pieces. At the first stage, a thermomechanical FEM model was implemented in the MSC.Marc commercial code in order to simulate the open die forging process. Starting from the results obtained through FEM simulations, a set of equations describing the plastic strain at the core of the piece have been identified depending on forging parameters (such as length of the contact surface between tools and ingot, tool’s connection radius, and reduction of the piece height after the forging pass). An Artificial Neural Network (ANN) was trained and tested in order to correlate the equation coefficients with the forging to obtain the behavior of plastic strain at the core of the piece.
A High Fat/High Sugar Diet Alters the Gastrointestinal Metabolome in a Sex Dependent Manner
Authors: Letsinger Ayland C. , Menon Rani , Iyer Anjushree R. , Vellers Heather L. , Granados Jorge Z. , Jayaraman Arul , Lightfoot J. Timothy
Published: October 2020

The gut metabolome offers insight for identifying the source of diet related pathology. As such, the purpose of this study was to characterize alterations of the gut metabolome in female and male C57BL/6J mice randomly assigned to a standard “chow” diet (CHOW) or a high fat/high sugar diet (HFHS; 45% fat and 20% fructose drinking solution) for nine weeks. Cecal metabolites were extracted and an untargeted analysis via LC-MS/MS was performed. Partial Least Sums Discriminate Analysis (PLS-DA) presented significant differences between the two diet groups in a sex-dependent manner. Mann–Whitney U-tests revealed 2443 and 1669 features to be significantly different between diet groups in the females and males, respectively. The majority of altered metabolites were depleted within the cecum of the HFHS fed mice. Metabolic pathways associated with galactose metabolism, leukotriene metabolism, and androgen and estrogen biosynthesis and metabolism were differentially altered with an HFHS diet between sexes. We concluded the immense metabolite depletion and elevation of adverse metabolites associated with the HFHS diet is suggestive of poor gut health. Further, the differential alterations between female and male mice suggests that sex plays an important role in determining the effect of diet on the metabolome and host health.
Improved Rectification and Osmotic Power in Polyelectrolyte-Filled Mesopores
Authors: Zheng Ding-Cheng , Yeh Li-Hsien
Published: October 2020

Ample studies have shown the use of nanofluidics in the ionic diode and osmotic power generation, but similar ionic devices performed with large-sized mesopores are still poorly understood. In this study, we model and realize the mesoscale ionic diode and osmotic power generator, composed of an asymmetric cone-shaped mesopore with its narrow opening filled with a polyelectrolyte (PE) layer with high space charges. We show that, only when the space charge density of a PE layer is sufficiently large (>1×106 C/m3), the considered mesopore system is able to create an asymmetric ionic distributions in the pore and then rectify ionic current. As a result, the output osmotic power performance can be improved when the filled PE carries sufficiently high space charges. For example, the considered PE-filled mesopore system can show an amplification of the osmotic power of up to 35.1-fold, compared to the bare solid-state mesopore. The findings provide necessary information for the development of large-sized ionic diode and osmotic power harvesting device.
Rhinovirus Infection in Children with Acute Bronchiolitis and Its Impact on Recurrent Wheezing and Asthma Development
Authors: Biagi Carlotta , Rocca Alessandro , Poletti Giulia , Fabi Marianna , Lanari Marcello
Published: October 2020

Acute bronchiolitis represents the leading cause of hospitalization in infants. Together with a respiratory syncytial virus, rhinovirus (RV) is one of the most common pathogens associated with bronchiolitis, and its genetic diversity (>150 types) makes the recurrence of RV infections each year quite typical. The frequency of RV infection and co-infection with other viruses and its impact on the clinical course of bronchiolitis have been studied by several authors with controversial results. Some studies demonstrate that multiple virus infections result in more severe clinical presentation and a higher risk of complications, whereas other studies suggest no influence on clinical course. Moreover, RV bronchiolitis has been reported to potentially contribute to the development of long-term sequelae, such as recurrent wheezing and asthma, in the pediatric population. In the present review, we summarize the most recent findings of the role of RV infection in children with acute bronchiolitis, its impact on subsequent asthma development, and the implication in clinical practice.
Effect of a Debaryomyces hansenii and Lactobacillus buchneri Starter Culture on Aspergillus westerdijkiae Ochratoxin A Production and Growth during the Manufacture of Short Seasoned Dry-Cured Ham
Authors: Iacumin Lucilla , Arnoldi Martina , Comi Giuseppe
Published: October 2020

Recently, specific dry-cured hams have started to be produced in San Daniele and Parma areas. The ingredients are similar to protected denomination of origin (PDO) produced in San Daniele or Parma areas, and include pork leg, coming from pigs bred in the Italian peninsula, salt and spices. However, these specific new products cannot be marked as a PDO, either San Daniele or Parma dry cured ham, because they are seasoned for 6 months, and the mark PDO is given only to products seasoned over 13 months. Consequently, these products are called short-seasoned dry-cured ham (SSDCH) and are not branded PDO. During their seasoning period, particularly from the first drying until the end of the seasoning period, many molds, including Eurotium spp. and Penicillium spp., can grow on the surface and work together with other molds and tissue enzymes to produce a unique aroma. Both of these strains typically predominate over other molds. However, molds producing ochratoxins, such as Aspergillus ochraceus and Penicillium nordicum, can simultaneously grow and produce ochratoxin A (OTA). Consequently, these dry-cured hams may represent a potential health risk for consumers. Recently, Aspergillus westerdijkiae has been isolated from SSDCHs, which could represent a potential problem for consumers. Therefore, the aim of this study was to inhibit A. westerdijkiae using Debaryomyces hansenii or Lactobacillus buchneri or a mix of both microorganisms. Six D. hansenii and six L. buchneri strains were tested in vitro for their ability to inhibit A. westerdijkiae. The strains D. hansenii (DIAL)1 and L. buchneri (Lb)4 demonstrated the highest inhibitory activity and were selected for in situ tests. The strains were inoculated or co-inoculated on fresh pork legs for SSDCH production with OTA-producing A. westerdijkiae prior to the first drying and seasoning. At the end of seasoning (six months), OTA was not detected in the SSDCH treated with both microorganisms and their combination. Because both strains did not adversely affect the SSDCH odor or flavor, the combination of these strains are proposed for use as starters to inhibit OTA-producing A. westerdijkiae.
A Review of Potential Impacts of Climate Change on Coffee Cultivation and Mycotoxigenic Fungi
Authors: Adhikari Mira , Isaac Elizabeth L. , Paterson R. Russell M. , Maslin Mark A.
Published: October 2020

Coffee is one of the most traded commodities in the world. It plays a significant role in the global economy, employing over 125 million people. However, it is possible that this vital crop is threatened by changing climate conditions and fungal infections. This paper reviews how suitable areas for coffee cultivation and the toxigenic fungi species of Aspergillus, Penicillium, and Fusarium will be affected due to climate change. By combining climate models with species distribution models, a number of studies have investigated the future distribution of coffee cultivation. Studies predict that suitable coffee cultivation area could drop by ~50% under representation concentration pathway (RCP) 6.0 by 2050 for both Arabica and Robusta. These findings agree with other studies which also see an altitudinal migration of suitable cultivation areas to cooler regions, but limited scope for latitudinal migration owing to coffee’s inability to tolerate seasonal temperature changes. Increased temperatures will see an overall increase in mycotoxin production such as aflatoxins, particularly in mycotoxigenic fungi (e.g., Aspergillus flavus) more suited to higher temperatures. Arabica and Robusta’s limited ability to relocate means both species will be grown in less suitable climates, increasing plant stress and making coffee more susceptible to fungal infection and mycotoxins. Information regarding climate change parameters with respect to mycotoxin concentrations in real coffee samples is provided and how the changed climate affects mycotoxins in non-coffee systems is discussed. In a few areas where relocating farms is possible, mycotoxin contamination may decrease due to the “parasites lost” phenomenon. More research is needed to include the effect of mycotoxins on coffee under various climate change scenarios, as currently there is a significant knowledge gap, and only generalisations can be made. Future modelling of coffee cultivation, which includes the influence of atmospheric carbon dioxide fertilisation and forest management, is also required; however, all indications show that climate change will have an extremely negative effect on future coffee production worldwide in terms of both a loss of suitable cultivation areas and an increase in mycotoxin contamination.
Sedimentology, Provenance and Radiometric Dating of the Silante Formation: Implications for the Cenozoic Evolution of the Western Andes of Ecuador
Authors: Vallejo Cristian , Almagor Santiago , Romero Christian , Herrera Jose L. , Escobar Vanessa , Spikings Richard A. , Winkler Wilfried , Vermeesch Pieter
Published: October 2020

The Silante Formation is a thick series of continental deposits, exposed along a trench-parallel distance of approximately 300 km within the Western Cordillera of Ecuador. The origin, tectonic setting, age and stratigraphic relationships are poorly known, although these are key to understand the Cenozoic evolution of the Ecuadorian Andes. We present new sedimentological, stratigraphic, petrographic, radiometric and provenance data from the Silante Formation and underlying rocks. The detailed stratigraphic analysis shows that the Silante Formation unconformably overlies Paleocene submarine fan deposits of the Pilalo Formation, which was coeval with submarine tholeiitic volcanism. The lithofacies of the Silante Formation suggest that the sediments were deposited in a debris flow dominated alluvial fan. Provenance analysis including heavy mineral assemblages and detrital zircon U-Pb ages indicate that sediments of the Silante Formation were derived from the erosion of a continental, calc-alkaline volcanic arc, pointing to the Oligocene to Miocene San Juan de Lachas volcanic arc. Thermochronological data and regional correlations suggest that deposition of the Silante Formation was coeval with regional rock and surface uplift of the Andean margin that deposited alluvial fans in intermontane and back-arc domains.
Cytotoxic, Antitumor and Toxicological Profile of Passiflora alata Leaf Extract
Authors: Amaral Ricardo G. , Gomes Silvana V. F. , Andrade Luciana N. , dos Santos Sara A. , Severino Patrícia , de Albuquerque Júnior Ricardo L. C. , Souto Eliana B. , Brandão Geraldo C. , Santos Sandra L. , David Jorge M. , Carvalho Adriana A.
Published: October 2020

Passiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (PaLE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. PaLE showed a CPI > 75% and greater potency (IC50 < 30 µg/mL) against PC3 and S180 cell lines. PaLE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that PaLE has antitumor activity in vitro and in vivo with low toxicity.
Effect of Enzymatic, Ultrasound, and Reflux Extraction Pretreatments on the Yield and Chemical Composition of Essential Oils
Authors: Miljanović Anđela , Bielen Ana , Grbin Dorotea , Marijanović Zvonimir , Andlar Martina , Rezić Tonči , Roca Sunčica , Jerković Igor , Vikić-Topić Dražen , Dent Maja
Published: October 2020

The effect of different hydrodistillation pretreatments, namely, reflux extraction, reflux extraction with the addition of cell wall-degrading enzymes, and ultrasound, on the yield and chemical composition of essential oils of sage, bay laurel, and rosemary was examined. All pretreatments improved essential oil yield compared to no-pretreatment control (40–64% yield increase), while the oil quality remained mostly unchanged (as shown by statistical analysis of GC-MS results). However, enzyme-assisted reflux extraction pretreatment did not significantly outperform reflux extraction (no-enzyme control), suggesting that the observed yield increase was mostly a consequence of reflux extraction and enzymatic activity had only a minute effect. Thus, we show that ultrasound and reflux extraction pretreatments are beneficial in the production of essential oils of selected Mediterranean plants, but the application of enzymes has to be carefully re-evaluated.
Electrical Properties Enhancement of Carbon Nanotube Yarns by Cyclic Loading
Authors: Weizman Orli , Mead Joey , Dodiuk Hanna , Kenig Samuel
Published: October 2020

Carbon nanotube yarns (CNTYs) possess low density, high conductivity, high strength, and moderate flexibility. These intrinsic properties allow them to be a preferred choice for use as conductive elements in high-performance composites. To fully exploit their potential as conductive reinforcing elements, further improvement in their electrical conductivity is needed. This study demonstrates that tensile cyclic loading under ambient conditions improves the electrical conductivity of two types of CNTYs. The results showed that the electrical resistance of untreated CNTYs was reduced by 80% using cyclic loading, reaching the resistance value of the drawn acid-treated CNTYs. Scanning electron microscopy showed that cyclic loading caused orientation and compaction of the CNT bundles that make up the CNTYs, resulting in significantly improved electrical conductivity of the CNTYs. Furthermore, the elastic modulus was increased by 20% while preserving the tensile strength. This approach has the potential to replace the environmentally unfriendly acid treatment currently used to enhance the conductivity of CNTYs.
Roles of NAD+ and Its Metabolites Regulated Calcium Channels in Cancer
Authors: Yu Peilin , Cai Xiaobo , Liang Yan , Wang Mingxiang , Yang Wei
Published: October 2020

Nicotinamide adenine dinucleotide (NAD+) is an essential cofactor for redox enzymes, but also moonlights as a regulator for ion channels, the same as its metabolites. Ca2+ homeostasis is dysregulated in cancer cells and affects processes such as tumorigenesis, angiogenesis, autophagy, progression, and metastasis. Herein, we summarize the regulation of the most common calcium channels (TRPM2, TPCs, RyRs, and TRPML1) by NAD+ and its metabolites, with a particular focus on their roles in cancers. Although the mechanisms of NAD+ metabolites in these pathological processes are yet to be clearly elucidated, these ion channels are emerging as potential candidates of alternative targets for anticancer therapy.
Synthesis of Benzimidazole–Based Analogs as Anti Alzheimer’s Disease Compounds and Their Molecular Docking Studies
Authors: Adalat Bushra , Rahim Fazal , Taha Muhammad , Alshamrani Foziah J. , Anouar El Hassane , Uddin Nizam , Shah Syed Adnan Ali , Ali Zarshad , Zakaria Zainul Amiruddin
Published: October 2020

We synthesized 10 analogs of benzimidazole-based thiosemicarbazide 1 (a–j) and 13 benzimidazole-based Schiff bases 2 (a–m), and characterized by various spectroscopic techniques and evaluated in vitro for acetylcholinesterase (AchE) and butyrylcholinesterase (BchE) inhibition activities. All the synthesized analogs showed varying degrees of acetylcholinesterase and butyrylcholinesterase inhibitory potentials in comparison to the standard drug (IC50 = 0.016 and 4.5 µM. Amongst these analogs 1 (a–j), compounds 1b, 1c, and 1g having IC50 values 1.30, 0.60, and 2.40 µM, respectively, showed good acetylcholinesterase inhibition when compared with the standard. These compounds also showed moderate butyrylcholinesterase inhibition having IC50 values of 2.40, 1.50, and 2.40 µM, respectively. The rest of the compounds of this series also showed moderate to weak inhibition. While amongst the second series of analogs 2 (a–m), compounds 2c, 2e, and 2h having IC50 values of 1.50, 0.60, and 0.90 µM, respectively, showed moderate acetylcholinesterase inhibition when compared to donepezil. Structure Aactivity Relation of both synthesized series has been carried out. The binding interactions between the synthesized analogs and the enzymes were identified through molecular docking simulations.
Screening of an Endophyte Transforming Polydatin to Resveratrol from Reynoutria Japonica Houtt and the Optimization of Its Transformation Parameters
Authors: Liu Jin , Zhang Xueqing , Yan Ting , Wang Faling , Li Jing , Jia Lingyun , Jia Jingming , Hu Gaosheng
Published: October 2020

Resveratrol showed various kinds of bioactivities, such as antioxidant, antimicrobial, anticancer effects and, therefore, has been used widely as an important ingredient in medication, healthy foods and cosmetics. However, in nature, resveratrol usually exists at low content and more often exists as polydatin. Therefore, it becomes important to find the cost-effective and environmental-friendly way to transform polydatin to resveratrol. In this study, endophytes were isolated from the rhizome tissue of Reynoutria japonica and screened for transforming polydatin to resveratrol using reversed-phase high-performance liquid chromatography (RP-HPLC) and confirmed by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy. A bacterium identified as Bacillus aryabhattai using 16S rRNA phylogenetic tree analysis showed highest transformation rate. The transforming conditions were optimized including substrate concentration, substrate addition time, culture temperature and inoculation ratio. Our results demonstrated that the bacteria isolated from R. japonica rhizome tissue showed high activity in transforming polydatin into resveratrol. Crude extract of R. japonica root and rhizome (RJE) was also tested as substrate and it was found that the transformation was significantly inhibited at 10.0 mg/mL RJE. Emodin at equivalent concentration of 10.0 mg/mL RJE showed no inhibition activity, and glucose content in RJE was trace and far from enough to exhibit the inhibitory activity. Successive solvent partition followed by an inhibition activity assay revealed that the ethyl acetate fraction showed the main inhibition activity. However, due to the coexistence of polydatin and compounds with inhibitory activity, the concentration of RJE can only be used at limited concentration as substrate.
Kinetic Study of Glucosamine Production Using Aspergillus sydowii BCRC 31742 under Solid-State Fermentation
Authors: Peng Jia Wei , Wu Ho Shing
Published: October 2020

In the present study, we aimed to obtain a high yield and productivity for glucosamine using a low-cost solid-state culture with Aspergillus sydowii BCRC 31742. The fermentation conditions, such as inoculum biomass, moisture content, and supplemental volume and mineral salt, were chosen to achieve high productivity of glucosamine (GlcN). When the initial supplemental volume used was 3 mL/g substrate, the yield and productivity of GlcN were 48.7 mg/gds and 0.69 mg/gds·h, respectively. This result will be helpful for the industrialization of the process.
Rab GTPase Mediating Regulation of NALP3 in Colorectal Cancer
Authors: Tezcan Gülçin , Garanina Ekaterina E. , Zhuravleva Margarita N. , Hamza Shaimaa , Rizvanov Albert A. , Khaiboullina Svetlana F.
Published: October 2020

The NALP3 inflammasome signaling contributes to inflammation within tumor tissues. This inflammation may be promoted by the vesicle trafficking of inflammasome components and cytokines. Rab5, Rab7 and Rab11 regulate vesicle trafficking. However, the role of these proteins in the regulation of inflammasomes remains largely unknown. To elucidate the role of these Rab proteins in inflammasome regulation, HCT-116, a colorectal cancer (CRC) cell line expressing pDsRed-Rab5 wild type (WT), pDsRed-Rab5 dominant-negative (DN), pDsRed-Rab7 WT, pDsRed-Rab7 DN, pDsRed-Rab11 WT and pDsRed-Rab11 DN were treated with lipopolysaccharide (LPS)/nigericin. Inflammasome activation was analyzed by measuring the mRNA expression of NLRP3, Pro-CASP1, RAB39A and Pro-IL-1β, conducting immunofluorescence imaging and western blotting of caspase-1 and analysing the secretion levels of IL-1β using enzyme-linked immunosorbent assay (ELISA). The effects of Rabs on cytokine release were evaluated using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Premixed 41 Plex. The findings showed that LPS/nigericin-treated cells expressing Rab5-WT indicated increased NALP3 expression and secretion of the IL-1β as compared to Rab5-DN cells. Caspase-1 was localized in the nucleus and cytosol of Rab5-WT cells but was localized in the cytosol in Rab5-DN cells. There were no any effects of Rab7 and Rab11 expression on the regulation of inflammasomes. Our results suggest that Rab5 may be a potential target for the regulation of NALP3 in the treatment of the CRC inflammation.
Affinity of Antifungal Isoxazolo[3,4-b]pyridine-3(1H)-Ones to Phospholipids in Immobilized Artificial Membrane (IAM) Chromatography
Authors: Ciura Krzesimir , Fedorowicz Joanna , Žuvela Petar , Lovrić Mario , Kapica Hanna , Baranowski Paweł , Sawicki Wiesław , Wong Ming Wah , Sączewski Jarosław
Published: October 2020

Currently, rapid evaluation of the physicochemical parameters of drug candidates, such as lipophilicity, is in high demand owing to it enabling the approximation of the processes of absorption, distribution, metabolism, and elimination. Although the lipophilicity of drug candidates is determined using the shake flash method (n-octanol/water system) or reversed phase liquid chromatography (RP-LC), more biosimilar alternatives to classical lipophilicity measurement are currently available. One of the alternatives is immobilized artificial membrane (IAM) chromatography. The present study is a continuation of our research focused on physiochemical characterization of biologically active derivatives of isoxazolo[3,4-b]pyridine-3(1H)-ones. The main goal of this study was to assess the affinity of isoxazolones to phospholipids using IAM chromatography and compare it with the lipophilicity parameters established by reversed phase chromatography. Quantitative structure–retention relationship (QSRR) modeling of IAM retention using differential evolution coupled with partial least squares (DE-PLS) regression was performed. The results indicate that in the studied group of structurally related isoxazolone derivatives, discrepancies occur between the retention under IAM and RP-LC conditions. Although some correlation between these two chromatographic methods can be found, lipophilicity does not fully explain the affinities of the investigated molecules to phospholipids. QSRR analysis also shows common factors that contribute to retention under IAM and RP-LC conditions. In this context, the significant influences of WHIM and GETAWAY descriptors in all the obtained models should be highlighted.
The Effect of Partial Corticotomy on the Rate of Maxillary Canine Retraction: Clinical and Radiographic Study
Authors: Baeshen Hosam
Published: October 2020

The study aimed to evaluate, clinically and radiographically, the effect of partial corticotomy of the buccal plate distal to the canine on the rate of maxillary canine retraction. A clinical trial with the split-mouth design was conducted among twenty orthodontic patients, recommended for first premolar extraction with an age range from 13 to 21 years, selected from patients seeking orthodontic treatment in private dental clinics in Jeddah, Kingdom of Saudi Arabia. After extraction of the maxillary right and left first premolar, partial corticotomy was performed distal to the canine on the right side. The canine retraction was carried out with a power chain on both sides extended between the canine and the maxillary first molar. The data collected from the current study were tabulated and statistically analyzed using an independent sample t-test with p < 0.05 considered statistically significant. The rate of canine retraction was significantly higher on the corticotomy side than the control side (p < 0.05). Under the limitations of the present study, it can be concluded that the technique of partial corticotomy of the buccal plate distal to the canine is a straightforward surgical procedure enhancing the rate of canine retraction significantly.
Lovastatin Differentially Regulates α7 and α4 Neuronal Nicotinic Acetylcholine Receptor Levels in Rat Hippocampal Neurons
Authors: Borroni Virginia , Kamerbeek Constanza , Pediconi María F. , Barrantes Francisco J.
Published: October 2020

Neuronal α7 and α4β2 are the predominant nicotinic acetylcholine receptor (nAChR) subtypes found in the brain, particularly in the hippocampus. The effects of lovastatin, an inhibitor of cholesterol biosynthesis, on these two nAChRs endogenously expressed in rat hippocampal neuronal cells were evaluated in the 0.01–1 µM range. Chronic (14 days) lovastatin treatment augmented cell-surface levels of α7 and α4 nAChRs, as measured by fluorescence microscopy and radioactive ligand binding assays. This was accompanied in both cases by an increase in total protein receptor levels as determined by Western blots. At low lovastatin concentrations (10–100 nM), the increase in α4 nAChR in neurites was higher than in neuronal cell somata; the opposite occurred at higher (0.5–1 µM) lovastatin concentrations. In contrast, neurite α7 nAChRs raised more than somatic α7 nAChRs at all lovastatin concentrations tested. These results indicate that cholesterol levels homeostatically regulate α7 and α4 nAChR levels in a differential manner through mechanisms that depend on statin concentration and receptor localization. The neuroprotective pleomorphic effects of statins may act by reestablishing the homeostatic equilibrium.