bluuebunny/crossref_metadata_2025 huggingface dataset:
Are Bank Employees Stressed? Job Perception and Positivity in the Banking Sector: An Italian Observational Study
Authors: Mannocci Alice , Marchini Laura , Scognamiglio Alfredo , Sinopoli Alessandra , De Sio Simone , Sernia Sabina , La Torre Giuseppe
Published: April 2018

Background: The epidemiology of stress on bank workers in Europe is only at the introductory stages. This study investigated for the first time the association between occupational stress level in bank-employees using the BEST8, Karasek-Model and socio-demographic and working factors in Italy. Methods: The observational pilot study involved 384 employees. Three questionnaires were adopted to collect data: Karasek-Model, BEST8 (p < 0.001) and Positivity-Scale. Results: 25% of the sample belonged to high stress group. The workers more stressed were older with a commercial role and consumer of antidepressants/sedatives. Women were much more likely to agree with the perception of feeling unsafe in a possible robbery (OR = 2.42; 95% CI: 1.50–3.91) and with that sales requests were in conflict with one’s own personal moral code (OR = 2.31; 95% CI: 1.38–3.87). Older employees declared feeling inadequate in the workplace (OR = 1.97; 95% CI: 1.07–3.65) and younger employees referred to be anxious about meeting financial budget goals. Workers who had a low positivity had a lower probability of adaptation (OR = 0.88; 95% CI: 0.83–0.93). Conclusions: The occupational stress level in the banking sector involves many aspects: gender, type of bank, role, personal morals, high job-demands, low level of decision-making. This study recommended that banks should implement strategic interventions for well-being of employees, and consequently for their productivity.
Hedgehog Signals Mediate Anti-Cancer Drug Resistance in Three-Dimensional Primary Colorectal Cancer Organoid Culture
Authors: Usui Tatsuya , Sakurai Masashi , Umata Koji , Elbadawy Mohamed , Ohama Takashi , Yamawaki Hideyuki , Hazama Shoichi , Takenouchi Hiroko , Nakajima Masao , Tsunedomi Ryouichi , Suzuki Nobuaki , Nagano Hiroaki , Sato Koichi , Kaneda Masahiro , Sasaki Kazuaki
Published: April 2018

Colorectal cancer is one of the most common causes of cancer death worldwide. In patients with metastatic colorectal cancer, combination treatment with several anti-cancer drugs is employed and improves overall survival in some patients. Nevertheless, most patients with metastatic disease are not cured owing to the drug resistance. Cancer stem cells are known to regulate resistance to chemotherapy. In the previous study, we established a novel three-dimensional organoid culture model from tumor colorectal tissues of human patients using an air–liquid interface (ALI) method, which contained numerous cancer stem cells and showed resistance to 5-fluorouracil (5-FU) and Irinotecan. Here, we investigate which inhibitor for stem cell-related signal improves the sensitivity for anti-cancer drug treatment in tumor ALI organoids. Treatment with Hedgehog signal inhibitors (AY9944, GANT61) decreases the cell viability of organoids compared with Notch (YO-01027, DAPT) and Wnt (WAV939, Wnt-C59) signal inhibitors. Combination treatment of AY9944 or GANT61 with 5-FU, Irinotecan or Oxaliplatin decreases the cell viability of tumor organoids compared with each anti-cancer drug alone treatment. Treatment with AY9944 or GANT61 inhibits expression of stem cell markers c-Myc, CD44 and Nanog, likely through the decrease of their transcription factor, GLI-1 expression. Combination treatment of AY9944 or GANT61 with 5-FU or Irinotecan also prevents colony formation of colorectal cancer cell lines HCT116 and SW480. These findings suggest that Hedgehog signals mediate anti-cancer drug resistance in colorectal tumor patient-derived ALI organoids and that the inhibitors are useful as a combinational therapeutic strategy against colorectal cancer.
Oligodendroglioma Cells Lack Glutamine Synthetase and Are Auxotrophic for Glutamine, but Do not Depend on Glutamine Anaplerosis for Growth
Authors: Chiu Martina , Taurino Giuseppe , Bianchi Massimiliano , Ottaviani Laura , Andreoli Roberta , Ciociola Tecla , Lagrasta Costanza , Tardito Saverio , Bussolati Ovidio
Published: April 2018

In cells derived from several types of cancer, a transcriptional program drives high consumption of glutamine (Gln), which is used for anaplerosis, leading to a metabolic addiction for the amino acid. Low or absent expression of Glutamine Synthetase (GS), the only enzyme that catalyzes de novo Gln synthesis, has been considered a marker of Gln-addicted cancers. In this study, two human cell lines derived from brain tumors with oligodendroglioma features, HOG and Hs683, have been shown to be GS-negative. Viability of both lines depends from extracellular Gln with EC50 of 0.175 ± 0.056 mM (Hs683) and 0.086 ± 0.043 mM (HOG), thus suggesting that small amounts of extracellular Gln are sufficient for OD cell growth. Gln starvation does not significantly affect the cell content of anaplerotic substrates, which, consistently, are not able to rescue cell growth, but causes hindrance of the Wnt/β-catenin pathway and protein synthesis attenuation, which is mitigated by transient GS expression. Gln transport inhibitors cause partial depletion of intracellular Gln and cell growth inhibition, but do not lower cell viability. Therefore, GS-negative human oligodendroglioma cells are Gln-auxotrophic but do not use the amino acid for anaplerosis and, hence, are not Gln addicted, exhibiting only limited Gln requirements for survival and growth.
Cytochrome P450 CYP6EV11 in Chironomus kiiensis Larvae Involved in Phenol Stress
Authors: Zhang Qihui , Chu Dong , Sun Lili , Cao Chuanwang
Published: April 2018

Phenol is one of the organic pollutants which can cause water environment pollution. It is not only enriched in aquatic organisms but is also a serious threat to human health. Chironomus kiiensis is very sensitive to the contaminants in water and its cytochrome P450s are usually chosen as biomarkers for water pollution. To examine whether CYP6EV11 plays a role in the oxidative metabolism of phenol, we measured the silencing efficiency of CYP6EV11 and evaluated larval susceptibility to sublethal phenol levels by RNA interference (RNAi) technology. The results showed that the transcription of CYP6EV11 was found significantly up-regulated when the 4th instar C. kiiensis larvae were exposed to three doses of phenol. However, the transcriptional levels of CYP6EV11 were significantly suppressed by 92.7% in the 4th instar C. kiiensis larvae soaked in dsCYP6EV11 compared with those soaked in dsGFP for 6 h. The CYP6EV11 expression and mortality of the 4th instar C. kiiensis larvae with CYP6EV11 silencing were mostly decreased under phenol stress. Therefore, the CYP6EV11 gene may be used as a molecular biomarker for earlier warning and monitoring for water pollution.
Ursodeoxycholate Restores Biliary Excretion of Methotrexate in Rats with Ethinyl Estradiol Induced-Cholestasis by Restoring Canalicular Mrp2 Expression
Authors: Kim Min , Kang Yun , Kwon Mihwa , Choi Young , Choi Min-Koo , Chi Hye-Young , Yoo Hye , Shim Chang-Koo , Song Im-Sook
Published: April 2018

The in vivo relevance of ursodeoxycholate (UDCA) treatment (100 mg/kg/day, per oral tid for 5 days before cholestasis induction followed by the same dosing for 5 days) on hepatic function was investigated in rats with 17α-ethinylestradiol (EE, 10 mg/kg, subcutaneous for 5 days)-induced experimental cholestasis. The bile flow rate and the expression level of hepatic multidrug resistance-associated protein 2 (Mrp 2) that were decreased in cholestasis were restored after UDCA treatment. Consistent with this, the biliary excretion clearance (CLexc,bile) of a representative Mrp2 substrate—methotrexate (MTX)—was decreased in cholestatic rats but was restored after UDCA treatment. Consequently, the plasma concentrations of MTX, which were increased by cholestasis, were decreased to control levels by UDCA treatment. Thus, the restoration of CLexc,bile appears to be associated with the increase in Mrp2 expression on the canalicular membrane by UDCA treatment followed by Mrp2-mediated biliary excretion of MTX. On the other hand, the hepatic uptake clearance (CLup,liver) of MTX was unchanged by cholestasis or UDCA treatment, suggestive of the absence of any association between the uptake process and the overall biliary excretion of MTX. Since UDCA has been known to induce the expression of canalicular MRP2 in humans, UDCA treatment might be effective in humans to maintain or accelerate the hepatobiliary elimination of xenobiotics or metabolic conjugates that are MRP2 substrates.
Instillation of Sericin Enhances Corneal Wound Healing through the ERK Pathway in Rat Debrided Corneal Epithelium
Authors: Nagai Noriaki , Fukuoka Yuya , Ishii Miyu , Otake Hiroko , Yamamoto Tetsushi , Taga Atsushi , Okamoto Norio , Shimomura Yoshikazu
Published: April 2018

Sericin is a major constituent of silk produced by silkworms. We previously found that the instillation of sericin enhanced the proliferation of corneal epithelial cells, and acted to promote corneal wound healing in both normal and diabetic model rats. However, the mechanisms by which sericin promotes the proliferation of corneal cells have not been established. In this study, we investigated the effects of sericin on Akt and ERK activation in a human corneal epithelial cell line (HCE-T cells) and rat debrided corneal epithelium. Although Akt phosphorylation was not detected following the treatment of HCE-T cells with sericin, ERK1/2 phosphorylation was enhanced. The growth of HCE-T cells treated with sericin was significantly increased, with the cell growth of sericin-treated HCE-T cells being 1.7-fold higher in comparison with vehicle-treated HCE-T cells. On the other hand, both of an ERK inhibitor U0126 (non-specific specific inhibitor) and SCH772984 (specific inhibitor) attenuated the enhanced cell growth by sericin, and the growth level in the case of co-treatment with sericin and ERK1/2 inhibitor was similar to that of cells treated with ERK1/2 inhibitor alone. In an in vivo study using rat debrided corneal epithelium, the corneal wound healing rate was enhanced by the instillation of sericin, and this enhancement was also attenuated by the instillation of U0126. In addition, the corneal wound healing rate in rats co-instilled with sericin and U0126 was similar to that following the instillation of U0126 alone. In conclusion, we found that the instillation of sericin enhanced cell proliferation via the activation of the MAPK/ERK pathway, resulting in the promotion of corneal wound healing in rat eyes. These findings provide significant information for designing further studies to develop potent corneal wound-healing drugs.
Comparative Genomics and Transcriptome Profiling in Primary Aldosteronism
Authors: Aristizabal Prada Elke , Castellano Isabella , Sušnik Eva , Yang Yuhong , Meyer Lucie , Tetti Martina , Beuschlein Felix , Reincke Martin , Williams Tracy
Published: April 2018

Primary aldosteronism is the most common form of endocrine hypertension with a prevalence of 6% in the general population with hypertension. The genetic basis of the four familial forms of primary aldosteronism (familial hyperaldosteronism FH types I–IV) and the majority of sporadic unilateral aldosterone-producing adenomas has now been resolved. Familial forms of hyperaldosteronism are, however, rare. The sporadic forms of the disease prevail and these are usually caused by either a unilateral aldosterone-producing adenoma or bilateral adrenal hyperplasia. Aldosterone-producing adenomas frequently carry a causative somatic mutation in either of a number of genes with the KCNJ5 gene, encoding an inwardly rectifying potassium channel, a recurrent target harboring mutations at a prevalence of more than 40% worldwide. Other than genetic variations, gene expression profiling of aldosterone-producing adenomas has shed light on the genes and intracellular signalling pathways that may play a role in the pathogenesis and pathophysiology of these tumors.
Crystal Structure of the Isocitrate Dehydrogenase 2 from Acinetobacter baumannii (AbIDH2) Reveals a Novel Dimeric Structure with Two Monomeric-IDH-Like Subunits
Authors: Wang Peng , Wu Yatao , Liu Jie , Song Ping , Li Shan , Zhou Xinxin , Zhu Guoping
Published: April 2018

Monomeric isocitrate dehydrogenases (IDHs) have a single polypeptide sizing around 85 kDa. The IDH2 from the opportunistic bacterium Acinetobacter baumannii (AbIDH2) with a mass of 83 kDa was formerly recognized as a typical monomeric IDH. However, both size exclusion chromatography and analytical ultracentrifugation analysis indicated that AbIDH2 exists as a homodimer in solution. The crystallographic study of the substrate/coenzyme-free AbIDH2 gave a dimeric structure and each subunit contained a domain I and a domain II. The dimeric assembly is mainly stabilized by hydrophobic interactions (16 hydrogen bonds and 11 salt bridges) from the dimer’s interface platform, which centered around the three parallel helices (α4, α12, and α17) and one loop from the domain II. Kinetic analysis showed that the dimeric AbIDH2 showed much lower catalytic efficiency (0.39 μM−1·s−1) as compared to the typical monomeric IDHs (~15 μM−1·s−1). Key residues crucial for dimer formation were simultaneously changed to generate the mutant mAbIDH2. The disruption of the hydrophobic forces disassociated the dimeric AbIDH2, making mAbIDH2 a monomeric enzyme. mAbIDH2 sustained specific activity (21.9 ± 2 U/mg) comparable to AbIDH2 (25.4 ± 0.7 U/mg). However, mAbIDH2 proved to be a thermolabile enzyme, indicating that the thermostable dimeric AbIDH2 may have a physiological significance for the growth and pathogenesis of A. baumannii. Phylogenetic analysis demonstrated the existence of numerous AbIDH2 homologous proteins, thus expanding the monomeric IDH protein family.
Neuroprotective Effects of Four Phenylethanoid Glycosides on H2O2-Induced Apoptosis on PC12 Cells via the Nrf2/ARE Pathway
Authors: Li Maiquan , Xu Tao , Zhou Fei , Wang Mengmeng , Song Huaxin , Xiao Xing , Lu Baiyi
Published: April 2018

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a key transcription factor against oxidative stress and neurodegenerative disorders. Phenylethanoid glycosides (PhGs; salidroside, acteoside, isoacteoside, and echinacoside) exhibit antioxidant and neuroprotective bioactivities. This study was performed to investigate the neuroprotective effect and molecular mechanism of PhGs. PhGs pretreatment significantly suppressed H2O2-induced cytotoxicity in PC12 cells by triggering the nuclear translocation of Nrf2 and reversing the downregulated protein expression of heme oxygenase 1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), glutamate cysteine ligase-catalytic subunit (GCLC), and glutamate-cysteine ligase modifier subunit (GCLM). Nrf2 siRNA or HO-1 inhibitor zinc protoporphyrin (ZnPP) reduced the neuroprotective effect. PhGs showed potential interaction with the Nrf2 binding site in Kelch-like ECH-association protein 1 (Keap1). This result may support the hypothesis that PhGs are activators of Nrf2. We demonstrated the potential binding between PhGs and the Keap1-activated Nrf2/ARE pathway, and that PhGs with more glycosides had enhanced effects.
Hybrid Destination-Based Jamming and Opportunistic Scheduling with Optimal Power Allocation to Secure Multiuser Untrusted Relay Networks
Authors: Ding Fan , Zhou Yongming
Published: April 2018

In this paper, we investigate secure communication for a dual-hop multiuser relay network, where a source communication with N ( N ≥ 1 ) destinations via an untrusted variable gains relay. To exploit multiuser diversity while protecting source’s confidential message, we first propose a joint destination-based jamming and opportunistic scheduling (DJOS) scheme. Then, we derive closed-form approximated and asymptotic expressions of the secrecy outage probability (SOP) for the considered system with DJOS. Furthermore, we determine an asymptotical optimal power allocation (OPA), which minimizes the asymptotic SOP, to further improve the secrecy performance. Our analytical results show that the achievable secrecy diversity order in terms of SOP with fixed power allocation is min ( 1 , N 2 ) , whereas, with OPA, the achievable secrecy diversity order can be improved up to min ( 1 , 2 N N + 2 ) . This interesting result reveals that OPA can improve the secrecy diversity order of the single-user network. This is intuitive since full diversity order of 1 cannot be achieved when N = 1 , thus leaving some space for OPA to improve the diversity order. Nevertheless, for N ≥ 2 , the effect of OPA is to increase the secrecy array gain rather than the secrecy diversity order since full diversity order 1 has been achieved by the OS scheme. Finally, simulation results are presented to validate our analysis.
The Effect of Topical Ocular Anesthetic Proparacaine on Conjunctival and Nasal Mucosal Flora in Dry Eye Disease Patients
Authors: Onerci Celebi Ozlem , Celebi Ali
Published: April 2018

The aim of this study was to investigate the effect of topically applied ocular anesthetic proparacaine on conjunctival and nasal bacterial mucosal flora in patients with dry eye disease. A Schirmer test was done with (group 1) and without (group 2) topical anesthetic proparacaine to 40 patients in each group. Conjunctival and nasal cultures were obtained before and 10 min after performing the Schirmer test. The bacterial culture results and the isolated bacteria were recorded in two groups. Patients’ mean age was 62 years (70 female, 10 male). Before the application of topical anesthetic, 50 (62.5%) and 62 (77.5%) had positive conjunctival and nasal culture, respectively, with the most commonly isolated organism being coagulase-negative Staphylococcus in each group. In group 1 the conjunctival bacterial culture positivity rate decreased from 26 (65%) to six (15%) eyes (p < 0.001); however, this rate decreased slightly from 24 (60%) to 20 (50%) eyes in group 2 (p > 0.05). For the nasal cultures, the bacterial culture positivity rate decreased from 80% to 20% and from 75% to 65% in groups 1 (p < 0.001) and 2 (p > 0.05), respectively. Topical ocular anesthetic proparacaine has antibacterial activity in both conjunctival and nasal flora in patients with dry eye disease.
Antiplatelet Therapy in Coronary Artery Disease: A Daunting Dilemma
Authors: Chaturvedula Surya , Diver Daniel , Vashist Aseem
Published: April 2018

Percutaneous coronary intervention (PCI) with stenting for the treatment of acute coronary syndrome (ACS) is the contemporary standard of care. Such treatment is followed by dual antiplatelet therapy (DAPT) comprising of aspirin and a P2Y12 inhibitor. The efficacy of this therapy has been well established but the optimal duration of DAPT remains elusive, and has thus far attracted a prodigious deal of scientific attention. The decision regarding DAPT duration can be clinically challenging in the modern era with the evolution of newer stents, more potent antiplatelet agents, and novel anticoagulant drugs in addition to an older patient population with multiple comorbidities. Major societal guidelines have emphasized comprehensive assessment of ischemic and bleeding risk, in turn recommending individualization of DAPT duration, thus encouraging “shared decision making”. The following review is aimed at critically evaluating the available evidence to help make these crucial clinical decisions regarding duration of DAPT and triple therapy.
Cellulose-Silica Nanocomposites of High Reinforcing Content with Fungi Decay Resistance by One-Pot Synthesis
Authors: Rodríguez-Robledo M. , González-Lozano M. , Ponce-Peña Patricia , Quintana Owen Patricia , Aguilar-González Miguel , Nieto-Castañeda Georgina , Bazán-Mora Elva , López-Martínez Rubén , Ramírez-Galicia Guillermo , Poisot Martha
Published: April 2018

Hybrid bionanocomposites based on cellulose matrix, with silica nanoparticles as reinforcers, were prepared by one-pot synthesis of cellulose surface modified by solvent exchange method to keep the biopolymer net void for hosting inorganic nanoparticles. Neither expensive inorganic-particle precursors nor crosslinker agents or catalysts were used for effective dispersion of reinforcer concentration up to 50 wt %. Scanning electron microscopy of the nanocomposites shows homogeneous dispersion of reinforcers in the surface modified cellulose matrix. The FTIR spectra demonstrated the cellulose features even at 50 weight percent content of silica nanoparticles. Such a high content of silica provides high thermal stability to composites, as seen by TGA-DSC. The fungi decay resistance to Trametes versicolor was measured by standard test showing good resistance even with no addition of antifungal agents. This one-pot synthesis of biobased hybrid materials represents an excellent way for industrial production of high performance materials, with a high content of inorganic nanoparticles, for a wide variety of applications.
A Novel Design Framework for Structures/Materials with Enhanced Mechanical Performance
Authors: Liu Jie , Fan Xiaonan , Wen Guilin , Qing Qixiang , Wang Hongxin , Zhao Gang
Published: April 2018

Structure/material requires simultaneous consideration of both its design and manufacturing processes to dramatically enhance its manufacturability, assembly and maintainability. In this work, a novel design framework for structural/material with a desired mechanical performance and compelling topological design properties achieved using origami techniques is presented. The framework comprises four procedures, including topological design, unfold, reduction manufacturing, and fold. The topological design method, i.e., the solid isotropic material penalization (SIMP) method, serves to optimize the structure in order to achieve the preferred mechanical characteristics, and the origami technique is exploited to allow the structure to be rapidly and easily fabricated. Topological design and unfold procedures can be conveniently completed in a computer; then, reduction manufacturing, i.e., cutting, is performed to remove materials from the unfolded flat plate; the final structure is obtained by folding out the plate from the previous procedure. A series of cantilevers, consisting of origami parallel creases and Miura-ori (usually regarded as a metamaterial) and made of paperboard, are designed with the least weight and the required stiffness by using the proposed framework. The findings here furnish an alternative design framework for engineering structures that could be better than the 3D-printing technique, especially for large structures made of thin metal materials.
Ultraviolet Laser Damage Dependence on Contamination Concentration in Fused Silica Optics during Reactive Ion Etching Process
Authors: Sun Laixi , Shao Ting , Shi Zhaohua , Huang Jin , Ye Xin , Jiang Xiaodong , Wu Weidong , Yang Liming , Zheng Wanguo
Published: April 2018

The reactive ion etching (RIE) process of fused silica is often accompanied by surface contamination, which seriously degrades the ultraviolet laser damage performance of the optics. In this study, we find that the contamination behavior on the fused silica surface is very sensitive to the RIE process which can be significantly optimized by changing the plasma generating conditions such as discharge mode, etchant gas and electrode material. Additionally, an optimized RIE process is proposed to thoroughly remove polishing-introduced contamination and efficiently prevent the introduction of other contamination during the etching process. The research demonstrates the feasibility of improving the damage performance of fused silica optics by using the RIE technique.
Waterborne Superhydrophobic and Superoleophobic Coatings for the Protection of Marble and Sandstone
Authors: Aslanidou Dimitra , Karapanagiotis Ioannis , Lampakis Dimitrios
Published: April 2018

Silica nanoparticles were dispersed in an aqueous emulsion of alkoxy silanes and organic fluoropolymer. The dispersion was sprayed onto white marble and sandstone. The deposited composite coatings exhibited (i) superhydrophobicity and superoleophobicity, as evidenced by the high (>150°) static contact angles of water and oil drops as well as (ii) water and oil repellency according to the low (<7°) corresponding tilt contact angles. Apart from marble and sandstone, the coatings with extreme wetting properties were deposited onto concrete, silk, and paper, thus demonstrating the versatility of the method. The siloxane/fluoropolymer product was characterized using Fourier Transform Infrared Spectroscopy (FT-IR), Raman spectroscopy and Scanning Electron Microscopy equipped with an Energy Dispersive X-ray Spectrometer (SEM-EDX). Moreover, SEM and FT-IR were used to reveal the surface structures of the composite coatings and their transition from superhydrophobicity to superhydrophilicity which occurred after severe thermal treatment. The composite coatings slightly reduced the breathability of marble and sandstone and had practically no optical effect on the colour of the two stones. Moreover, the coatings offered good protection against water penetration by capillarity.
AlgM4: A New Salt-Activated Alginate Lyase of the PL7 Family with Endolytic Activity
Authors: Huang Guiyuan , Wang Qiaozhen , Lu Mingqian , Xu Chao , Li Fei , Zhang Rongcan , Liao Wei , Huang Shushi
Published: April 2018

Alginate lyases are a group of enzymes that catalyze the depolymerization of alginates into oligosaccharides or monosaccharides. These enzymes have been widely used for a variety of purposes, such as producing bioactive oligosaccharides, controlling the rheological properties of polysaccharides, and performing structural analyses of polysaccharides. The algM4 gene of the marine bacterium Vibrio weizhoudaoensis M0101 encodes an alginate lyase that belongs to the polysaccharide lyase family 7 (PL7). In this study, the kinetic constants Vmax (maximum reaction rate) and Km (Michaelis constant) of AlgM4 activity were determined as 2.75 nmol/s and 2.72 mg/mL, respectively. The optimum temperature for AlgM4 activity was 30 °C, and at 70 °C, AlgM4 activity dropped to 11% of the maximum observed activity. The optimum pH for AlgM4 activity was 8.5, and AlgM4 was completely inactive at pH 11. The addition of 1 mol/L NaCl resulted in a more than sevenfold increase in the relative activity of AlgM4. The secondary structure of AlgM4 was altered in the presence of NaCl, which caused the α-helical content to decrease from 12.4 to 10.8% and the β-sheet content to decrease by 1.7%. In addition, NaCl enhanced the thermal stability of AlgM4 and increased the midpoint of thermal denaturation (Tm) by 4.9 °C. AlgM4 exhibited an ability to degrade sodium alginate, poly-mannuronic acid (polyM), and poly-guluronic acid (polyG), resulting in the production of oligosaccharides with a degree of polymerization (DP) of 2–9. AlgM4 possessed broader substrate, indicating that it is a bifunctional alginate lyase. Thus, AlgM4 is a novel salt-activated and bifunctional alginate lyase of the PL7 family with endolytic activity.
Holotoxin A1 Induces Apoptosis by Activating Acid Sphingomyelinase and Neutral Sphingomyelinase in K562 and Human Primary Leukemia Cells
Authors: Yun Seong-Hoon , Sim Eun-Hye , Han Sang-Heum , Han Jin-Yeong , Kim Sung-Hyun , Silchenko Alexandra , Stonik Valentin , Park Joo-In
Published: April 2018

Marine triterpene glycosides are attractive candidates for the development of anticancer agents. Holotoxin A1 is a triterpene glycoside found in the edible sea cucumber, Apostichopus (Stichopus) japonicus. We previously showed that cladoloside C2, the 25(26)-dihydro derivative of holotoxin A1, induced apoptosis in human leukemia cells by activating ceramide synthase 6. Thus, we hypothesized that holotoxin A1, which is structurally similar to cladoloside C2, might induce apoptosis in human leukemia cells through the same molecular mechanism. In this paper, we compared holotoxin A1 and cladoloside C2 for killing potency and mechanism of action. We found that holotoxin A1 induced apoptosis more potently than cladoloside C2. Moreover, holotoxin A1 induced apoptosis in K562 cells by activating caspase-8 and caspase-3, but not by activating caspase-9. During holotoxin A1-induced apoptosis, acid sphingomyelinase (SMase) and neutral SMase were activated in both K562 cells and human primary leukemia cells. Specifically inhibiting acid SMase and neutral SMаse with chemical inhibitors or siRNAs significantly inhibited holotoxin A1–induced apoptosis. These results indicated that holotoxin A1 might induce apoptosis by activating acid SMase and neutral SMase. In conclusion, holotoxin A1 represents a potential anticancer agent for treating leukemia. Moreover, the aglycone structure of marine triterpene glycosides might affect the mechanism involved in inducing apoptosis.
Effects of N2 Partial Pressure on Growth, Structure, and Optical Properties of GaN Nanorods Deposited by Liquid-Target Reactive Magnetron Sputter Epitaxy
Authors: Junaid Muhammad , Hsiao Ching-Lien , Chen Yen-Ting , Lu Jun , Palisaitis Justinas , Persson Per , Hultman Lars , Birch Jens
Published: April 2018

GaN nanorods, essentially free from crystal defects and exhibiting very sharp band-edge luminescence, have been grown by reactive direct-current magnetron sputter epitaxy onto Si (111) substrates at a low working pressure of 5 mTorr. Upon diluting the reactive N2 working gas with a small amount of Ar (0.5 mTorr), we observed an increase in the nanorod aspect ratio from 8 to ~35, a decrease in the average diameter from 74 to 35 nm, and a two-fold increase in nanorod density. With further dilution (Ar = 2.5 mTorr), the aspect ratio decreased to 14, while the diameter increased to 60 nm and the nanorod density increased to a maximum of 2.4 × 109 cm−2. Yet, lower N2 partial pressures eventually led to the growth of continuous GaN films. The observed morphological dependence on N2 partial pressure is explained by a change from N-rich to Ga-rich growth conditions, combined with reduced GaN-poisoning of the Ga-target as the N2 gas pressure is reduced. Nanorods grown at 2.5 mTorr N2 partial pressure exhibited a high intensity 4 K photoluminescence neutral donor bound exciton transitions (D0XA) peak at ~3.479 eV with a full-width-at-half-maximum of 1.7 meV. High-resolution transmission electron microscopy corroborated the excellent crystalline quality of the nanorods.
Hybrid Drug Delivery Patches Based on Spherical Cellulose Nanocrystals and Colloid Titania—Synthesis and Antibacterial Properties
Authors: Evdokimova Olga , Svensson Fredric , Agafonov Alexander , Håkansson Sebastian , Seisenbaeva Gulaim , Kessler Vadim
Published: April 2018

Spherical cellulose nanocrystal-based hybrids grafted with titania nanoparticles were successfully produced for topical drug delivery. The conventional analytical filter paper was used as a precursor material for cellulose nanocrystals (CNC) production. Cellulose nanocrystals were extracted via a simple and quick two-step process based on first the complexation with Cu(II) solution in aqueous ammonia followed by acid hydrolysis with diluted H2SO4. Triclosan was selected as a model drug for complexation with titania and further introduction into the nanocellulose based composite. Obtained materials were characterized by a broad variety of microscopic, spectroscopic, and thermal analysis methods. The drug release studies showed long-term release profiles of triclosan from the titania based nanocomposite that agreed with Higuchi model. The bacterial susceptibility tests demonstrated that released triclosan retained its antibacterial activity against Escherichia coli and Staphylococcus aureus. It was found that a small amount of titania significantly improved the antibacterial activity of obtained nanocomposites, even without immobilization of model drug. Thus, the developed hybrid patches are highly promising candidates for potential application as antibacterial agents.
Proteomic Profiles of Adipose and Liver Tissues from an Animal Model of Metabolic Syndrome Fed Purple Vegetables
Authors: Ayoub Hala , McDonald Mary , Sullivan James , Tsao Rong , Meckling Kelly
Published: April 2018

Metabolic Syndrome (MetS) is a complex disorder that predisposes an individual to Cardiovascular Diseases and type 2 Diabetes Mellitus. Proteomics and bioinformatics have proven to be an effective tool to study complex diseases and mechanisms of action of nutrients. We previously showed that substitution of the majority of carbohydrate in a high fat diet by purple potatoes (PP) or purple carrots (PC) improved insulin sensitivity and hypertension in an animal model of MetS (obese Zucker rats) compared to a control sucrose-rich diet. In the current study, we used TMT 10plex mass tag combined with LC-MS/MS technique to study proteomic modulation in the liver (n = 3 samples/diet) and adipose tissue (n = 3 samples/diet) of high fat diet-fed rats with or without substituting sucrose for purple vegetables, followed by functional enrichment analysis, in an attempt to elucidate potential molecular mechanisms responsible for the phenotypic changes seen with purple vegetable feeding. Protein folding, lipid metabolism and cholesterol efflux were identified as the main modulated biological themes in adipose tissue, whereas lipid metabolism, carbohydrate metabolism and oxidative stress were the main modulated themes in liver. We propose that enhanced protein folding, increased cholesterol efflux and higher free fatty acid (FFA) re-esterification are mechanisms by which PP and PC positively modulate MetS pathologies in adipose tissue, whereas, decreased de novo lipogenesis, oxidative stress and FFA uptake, are responsible for the beneficial effects in liver. In conclusion, we provide molecular evidence for the reported metabolic health benefits of purple carrots and potatoes and validate that these vegetables are good choices to replace other simple carbohydrate sources for better metabolic health.
Association between Urinary Aflatoxin (AFM1) and Dietary Intake among Adults in Hulu Langat District, Selangor, Malaysia
Authors: Sulaiman Siti , Jamaluddin Rosita , Sabran Mohd
Published: April 2018

Aflatoxin is a food contaminant and its exposure through the diet is frequent and ubiquitous. A long-term dietary aflatoxin exposure has been linked to the development of liver cancer in populations with high prevalence of aflatoxin contamination in foods. Therefore, this study was conducted to identify the association between urinary aflatoxin M1 (AFM1), a biomarker of aflatoxin exposure, with the dietary intake among adults in Hulu Langat district, Selangor, Malaysia. Certain food products have higher potential for aflatoxin contamination and these were listed in a Food Frequency Questionnaire, which was given to all study participants. This allowed us to record consumption rates for each food product listed. Concomitantly, urine samples were collected, from adults in selected areas in Hulu Langat district, for the measurement of AFM1 levels using an ELISA kit. Of the 444 urine samples collected and tested, 199 were positive for AFM1, with 37 of them exceeding the limit of detection (LOD) of 0.64 ng/mL. Cereal products showed the highest consumption level among all food groups, with an average intake of 512.54 g per day. Chi-square analysis showed that consumption of eggs (X2 = 4.77, p = 0.03) and dairy products (X2 = 19.36, p < 0.01) had significant associations with urinary AFM1 but both food groups were having a phi and Cramer’s V value that less than 0.3, which indicated that the association between these food groups’ consumption and AFM1 level in urine was weak.
A Mediterranean Diet Model in Australia: Strategies for Translating the Traditional Mediterranean Diet into a Multicultural Setting
Authors: George Elena S. , Kucianski Teagan , Mayr Hannah L. , Moschonis George , Tierney Audrey C. , Itsiopoulos Catherine
Published: April 2018

Substantial evidence supports the effect of the Mediterranean Diet (MD) for managing chronic diseases, although trials have been primarily conducted in Mediterranean populations. The efficacy and feasibility of the Mediterranean dietary pattern for the management of chronic diseases has not been extensively evaluated in non-Mediterranean settings. This paper aims to describe the development of a MD model that complies with principles of the traditional MD applied in a multiethnic context. Optimal macronutrient and food-based composition was defined, and a two-week menu was devised incorporating traditional ingredients with evidence based on improvements in chronic disease management. Strategies were developed for the implementation of the diet model in a multiethnic population. Consistent with the principles of a traditional MD, the MD model was plant-based and high in dietary fat, predominantly monounsaturated fatty acids from extra virgin olive oil. Fruits, vegetables and wholegrains were a mainstay, and moderate amounts of nuts and seeds, fish, dairy and red wine were recommended. The diet encompassed key features of the MD including cuisine, biodiversity and sustainability. The MD model preserved traditional dietary components likely to elicit health benefits for individuals with chronic diseases, even with the adaptation to an Australian multiethnic population.
Computational Modelling of Large Scale Phage Production Using a Two-Stage Batch Process
Authors: Krysiak-Baltyn Konrad , Martin Gregory , Gras Sally
Published: April 2018

Cost effective and scalable methods for phage production are required to meet an increasing demand for phage, as an alternative to antibiotics. Computational models can assist the optimization of such production processes. A model is developed here that can simulate the dynamics of phage population growth and production in a two-stage, self-cycling process. The model incorporates variable infection parameters as a function of bacterial growth rate and employs ordinary differential equations, allowing application to a setup with multiple reactors. The model provides simple cost estimates as a function of key operational parameters including substrate concentration, feed volume and cycling times. For the phage and bacteria pairing examined, costs and productivity varied by three orders of magnitude, with the lowest cost found to be most sensitive to the influent substrate concentration and low level setting in the first vessel. An example case study of phage production is also presented, showing how parameter values affect the production costs and estimating production times. The approach presented is flexible and can be used to optimize phage production at laboratory or factory scale by minimizing costs or maximizing productivity.
Polyethylene-Grafted Gold and Silver Nanoparticles Using Catalyzed Chain Growth (CCG)
Authors: Wagner Jannik , Peng Wentao , Vana Philipp
Published: April 2018

We report an efficient synthesis route for the formation of gold/silver-core–PE-shell nanohybrids in a simple self-assembly approach using PE with strong aurophilicity and argentophilicity, via thiol- and trithiocarbonate terminated moieties. This united the unique properties of polyethylene (PE) with gold and silver nanoparticles, using the well-defined end-group design of PE. These nanocomposites showed a similar solubility as PE, as confirmed by dynamic light scattering, and could be fully incorporated into a polyethylene matrix with different particle contents, as visualized by transmission electron microscopy. Using UV/vis-spectroscopy, we observed reversible, thermoresponsive aggregation/deaggregation properties in the nanohybrids, validating the strong and effective anchoring of PE on gold/silver surfaces.
How Glycerol and Water Contents Affect the Structural and Functional Properties of Starch-Based Edible Films
Authors: Basiak Ewelina , Lenart Andrzej , Debeaufort Frédéric
Published: April 2018

As starch is an inexpensive, filmogenic, easily processable and a widely available material, it is a material that can be utilized in the creation of biodegradable films and containers, presenting as a viable alternative to polymers derived from petrol. Moreover, starch could also be used to create edible coatings for fresh foods in order to extend shelf life. As such, wheat starch films with two glycerol contents were formulated to mimic the effects of compounds currently used to coat fruit. Their structural and functional properties were characterized. This study found that the transfer properties of starch films containing 33% of plasticizer was less effective than film comprised of 50% glycerol. Water diffusivity, oxygen permeability, and water vapor permeability at two different humidity gradients, surface tension, works of surface adhesion and cohesion, and moisture sorption were tested. Glycerol content does not play a significant role on the color or mechanical properties. This work shows that glycerol can strongly affect the functional properties of starch-based coatings and films.
Effect of Polysiloxanes on Roughness and Durability of Basalt Fibres–Reinforced Cement Mortar
Authors: Barnat-Hunek Danuta , Łagód Grzegorz , Fic Stanisław , Jarosz-Hadam Monika
Published: April 2018

The influence of roughness and the way it affects the adhesion properties and surface free energy (SFE) of polysiloxanes hydrophobised basalt fibres–reinforced cement mortars were determined in this article. The physical properties of mortars were investigated in the experimental part, which also explored the impact of hydrophobisation and basalt fibres (BF) addition on SFE, frost resistance, contact angle (CA), and roughness. A device capable of calculating all parameters was used to indicate the surface roughness and 3D topography. Prior to and after conducting surface and weight hydrophobisation, the contact angle of mortars was specified. Subsequently, it was used for carrying out SFE calculation by means of Neumann’s method, enabling us to characterize the adhesion properties and wettability of mortars. The research indicated that the surface roughness was substantially decreased, in turn raising the frost resistance. The corrosion resistance drops when the surface roughness, water absorption, and number of fibres in the mortar increase. The SEM images presenting the structure of polysiloxane coating and mortars were provided.
Classification of High-Mountain Vegetation Communities within a Diverse Giant Mountains Ecosystem Using Airborne APEX Hyperspectral Imagery
Authors: Marcinkowska-Ochtyra Adriana , Zagajewski Bogdan , Raczko Edwin , Ochtyra Adrian , Jarocińska Anna
Published: April 2018

Mapping plant communities is a difficult and time consuming endeavor. Methods relying on field surveys deliver high quality data but are usually limited to relatively small areas. In this paper we apply airborne hyperspectral data to vegetation mapping in remote and hard to reach areas. We classified 22 vegetation communities in the Giant Mountains on 3.12-m Airborne Prism Experiment (APEX) hyperspectral images, registered in 288 spectral bands (10 September 2012). As the classification algorithm, Support Vector Machines (SVM) was used. APEX data were corrected geometrically and atmospherically, and three dimensionality reduction methods were performed to select the best dataset. As reference we used a non-forest vegetation map containing vegetation communities of Polish Karkonosze National Park from 2002, orthophotomap and field surveys data from 2013 to 2014. We obtained the post-classification maps of 22 vegetation communities, lakes and areas without any vegetation. Iterative accuracy assessment repeated 100 times was used to obtain the most objective results for individual communities. The median value of overall accuracy (OA) was 84%. Fourteen out of twenty-four classes were classified of more than 80% of producer accuracy (PA) and sixteen out of twenty-four of user accuracy (UA). APEX data and SVM with the use of iterative accuracy assessment are useful for the mountain communities classification. This can support both Polish and Czech national parks management by giving the information about diversity of communities in the whole transboundary area, helping with identification especially in changing environment caused by humans.
Validation of MODIS C6 Dark Target Aerosol Products at 3 km and 10 km Spatial Resolutions Over the China Seas and the Eastern Indian Ocean
Authors: Shen Xiaojing , Bilal Muhammad , Qiu Zhongfeng , Sun Deyong , Wang Shengqiang , Zhu Weijun
Published: April 2018

In this study, MODerate resolution Imaging Spectroradiometer (MODIS) Collection 6 (C6) level-2 Dark Target (DT) Aerosol Optical Depth (AOD) products at 3 km (DT3K) and 10 km (DT10K) spatial resolutions were validated over the China seas and the eastern Indian Ocean against Maritime Aerosol Network (MAN) Level 1.5 AOD measurements collected through 13 cruises from 2010 to 2014. For this, DT3K and DT10K AOD observations were obtained from four Scientific Data Sets (SDS), i.e., “Effective_Optical_Depth_Average_Ocean” (EODAOAOD), “Effective_Optical_Depth_Best_Ocean” (EODBOAOD), “Image_Optical_Depth_Land_And_Ocean” (IODLAOAOD) and “Optical_Depth_Land_And_Ocean” (ODLAOAOD). The MAN AOD measurements were filtered within (i) ±2 h, (ii) ±4 h, (iii) ±6 h, and (iv) ±12 h of MODIS overpass time. Results showed that the DT10K and DT3K performed equally over the China seas and the eastern Indian Ocean in terms of retrievals quality and agreement with the MAN AOD measurements, whereas the DT3K has less coincident observations than the DT10K. For seasonal analysis, larger underestimation in the DT algorithm was observed in autumn followed by spring, whereas retrievals were well correlated with the MAN AOD data in summer. Overall, this study found that ODLAOAOD observations for the DT3K and DT10K were much better than EODAOAOD, EODBOAOD and IODLAOAOD in terms of high correlation and a large percentage of the AOD retrievals within the Expected Error (EE = +(0.04 + 10%), −(0.02 + 10%)).
3-D Characterization of Vineyards Using a Novel UAV Imagery-Based OBIA Procedure for Precision Viticulture Applications
Authors: De Castro Ana , Jiménez-Brenes Francisco , Torres-Sánchez Jorge , Peña José , Borra-Serrano Irene , López-Granados Francisca
Published: April 2018

Precision viticulture has arisen in recent years as a new approach in grape production. It is based on assessing field spatial variability and implementing site-specific management strategies, which can require georeferenced information of the three dimensional (3D) grapevine canopy structure as one of the input data. The 3D structure of vineyard fields can be generated applying photogrammetric techniques to aerial images collected with Unmanned Aerial Vehicles (UAVs), although processing the large amount of crop data embedded in 3D models is currently a bottleneck of this technology. To solve this limitation, a novel and robust object-based image analysis (OBIA) procedure based on Digital Surface Model (DSM) was developed for 3D grapevine characterization. The significance of this work relies on the developed OBIA algorithm which is fully automatic and self-adaptive to different crop-field conditions, classifying grapevines, and row gap (missing vine plants), and computing vine dimensions without any user intervention. The results obtained in three testing fields on two different dates showed high accuracy in the classification of grapevine area and row gaps, as well as minor errors in the estimates of grapevine height. In addition, this algorithm computed the position, projected area, and volume of every grapevine in the field, which increases the potential of this UAV- and OBIA-based technology as a tool for site-specific crop management applications.