bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure S1 from Phase II Trial of HER-Vaxx, a B-cell Peptide-Based Vaccine, in HER2-Overexpressing Advanced Gastric Cancer Patients Under Platinum-Based Chemotherapy (HERIZON)
Authors: Tobias Joshua , Maglakelidze Marina , Andrić Zoran , Ryspayeva Dinara , Bulat Iurie , Nikolić Ivan , Petrović Zoran , Chawla Tanuj , Nagarkar Rajnish , Garner-Spitzer Erika , Zielinski Christoph C. , Chong Leslie Mi Ok , Nixon Bonnie , Ede Nicholas J. , Yavrom Sharon , Kundi Michael , Wiedermann Ursula
Published: September 2024

Supplementary Figure S1. Trial profile and patient disposition.
Supplementary Figure 6 from Precision Oncology and Systemic Targeted Therapy in Pseudomyxoma Peritonei
Authors: Martínez-Quintanilla Jordi , Cabot Débora , Sabia Doménico , Arqués Oriol , Vergés Jordi , Chicote Irene , Bijelic Lana , Cabellos Laia , Alcántara Anna M. , Ramos Isabel , Barrios Pedro , Crusellas Oriol , Palacio Lina M. , Cámara Juan A. , Barriuso Jorge , Jiménez Juan J. , Muñoz-Torres Pau , Nonell Lara , Flores Raquel , Médico Enzo , Guaglio Marcello , Ros Javier , Élez Elena , Tabernero Josep , Aziz Omer , Deraco Marcello , Palmer Héctor G. , Giovanna Chiorino , Francesco Mazzarotto , Manuela Gariboldi , Luca Varinelli , Tommaso Cavalleri , Bipasha Chakrabarty , Raghavendar Nagaraju , Patrick Caswell , Milly McAllister
Published: September 2024

Supplementary Figure 6. Treatment with KRASG12D inhibitor reduces cell viability in KRASG12D mutant PMP-PDXO model in a dose dependent manner.
Supplementary Figure 10 from Precision Oncology and Systemic Targeted Therapy in Pseudomyxoma Peritonei
Authors: Martínez-Quintanilla Jordi , Cabot Débora , Sabia Doménico , Arqués Oriol , Vergés Jordi , Chicote Irene , Bijelic Lana , Cabellos Laia , Alcántara Anna M. , Ramos Isabel , Barrios Pedro , Crusellas Oriol , Palacio Lina M. , Cámara Juan A. , Barriuso Jorge , Jiménez Juan J. , Muñoz-Torres Pau , Nonell Lara , Flores Raquel , Médico Enzo , Guaglio Marcello , Ros Javier , Élez Elena , Tabernero Josep , Aziz Omer , Deraco Marcello , Palmer Héctor G. , Giovanna Chiorino , Francesco Mazzarotto , Manuela Gariboldi , Luca Varinelli , Tommaso Cavalleri , Bipasha Chakrabarty , Raghavendar Nagaraju , Patrick Caswell , Milly McAllister
Published: September 2024

Supplementary Figure 10. BRAF inhibitor treatment impacts in the mucinous structure of PMP tumors.
Figure 4 from Precision Oncology and Systemic Targeted Therapy in Pseudomyxoma Peritonei
Authors: Martínez-Quintanilla Jordi , Cabot Débora , Sabia Doménico , Arqués Oriol , Vergés Jordi , Chicote Irene , Bijelic Lana , Cabellos Laia , Alcántara Anna M. , Ramos Isabel , Barrios Pedro , Crusellas Oriol , Palacio Lina M. , Cámara Juan A. , Barriuso Jorge , Jiménez Juan J. , Muñoz-Torres Pau , Nonell Lara , Flores Raquel , Médico Enzo , Guaglio Marcello , Ros Javier , Élez Elena , Tabernero Josep , Aziz Omer , Deraco Marcello , Palmer Héctor G. , Giovanna Chiorino , Francesco Mazzarotto , Manuela Gariboldi , Luca Varinelli , Tommaso Cavalleri , Bipasha Chakrabarty , Raghavendar Nagaraju , Patrick Caswell , Milly McAllister
Published: September 2024

BRAF inhibitor suppresses subcutaneous tumor growth in a BRAFV600E PMP-PDX model. BRAFV600E mutant PMP-PDX cells from PMP5.1 were subcutaneously implanted in mice and treated with vehicle, intraperitoneal cetuximab (20 mg/kg), oral encorafenib (20 mg/kg), or doublet (n = 10–12/group). A, Tumor growth curve of the treated mice overtime. Mean ± SEM is shown. B, Graphs show the Western blot quantification of pEGFR/EGFR (left) and pERK/ERK (right) ratio from samples of representative excised tumors at day 4 after treatment. Mean ± SD is shown. C, Representation of the fold change (FC) in tumor volume for each tumor at day 44 after treatment. Mean ± SEM is shown. Significant differences were assessed using one-way ANOVA and Dunnett’s multiple comparisons tests compared to vehicle group (*, P value < 0.05; ****, P value < 0.0001; A and C). D, Image of representative tumors from each group at the end of the experiment. E, Survival curves represent PFS percentage of each tumor. Mice survival was evaluated by the Kaplan–Meier survival curve and log-rank test group (****, P value < 0.0001). F, Ki-67 IHC was performed from all tumor samples at the end of the experiment. Images from a representative tumor from vehicle, cetuximab, encorafenib and doublet groups. Scale bar, 100 μm. PMP, Pseudomyxoma peritonei; PDX, Patient-derived xenografts.
Data from Associations of Immune Checkpoint Predictive Biomarkers (MHC-I and MHC-II) with Clinical and Molecular Features in a Diverse Breast Cancer Cohort
Authors: Sun Xiaopeng , Kennedy Laura C. , Gonzalez-Ericsson Paula I. , Sanchez Violeta , Sanders Melinda , Perou Charles M. , Troester Melissa A. , Balko Justin M. , Reid Sonya A.
Published: September 2024

AbstractPurpose:
Immunotherapy (IO) in triple-negative breast cancer (TNBC) has improved survival outcomes, with promising improvements in pCR rates among early high-risk hormone receptor (HR)+/HER2− breast cancers. However, biomarkers are needed to select patients likely to benefit from IO. MHC-I and tumor-specific MHC-II (tsMHC-II) expression are candidate biomarkers for PD-(L)1 checkpoint inhibition but existing data from clinical trials included limited racial/ethnic diversity.
Experimental Design:
We performed multiplexed immunofluorescence assays in the Carolina Breast Cancer Study (CBCS; n = 1,628, 48% Black, 52% non-Black). Intrinsic subtype and P53 mutant-like status were identified using RNA-based multigene assays. We ranked participants based on tumoral MHC-I intensity (top 33% categorized as “MHC-Ihigh”) and MHC-II+ (≥5% of tumor cells as tsMHC-II+). MHC-I/II were evaluated in association with clinicopathological features by race.
Results:
Black participants had higher frequency of TNBC (25% vs. 12.5%, P ≤ 0.001) and basal-like (30% vs. 14%, P ≤ 0.001) tumors overall, and higher frequency of basal-like (11% vs. 5.5%, P = 0.002) and TP53 mutant tumors (26% vs. 17%, P = 0.002) among HR+/HER2−. The frequency of tsMHC-II+ was higher in HR+/HER2− Black participants (7.9% vs. 4.9%, P = 0.04). Black participants also had higher frequency of MHC-Ihigh (38.7% vs. 28.2%, P < 0.001), which was significant among HR+/HER2− (28.2% vs. 22.1%, P = 0.02).
Conclusions:
In this diverse study population, MHC-I and MHC-II tumor cell expression were more highly expressed in HR+/HER2− tumors from Black women, underscoring the importance of diverse and equitable enrollment in future IO trials.
Data from Interim Phase II Results Using Panitumumab-IRDye800CW during Transoral Robotic Surgery in Patients with Oropharyngeal Cancer
Authors: Stone Logan D. , Kasten Benjamin B. , Rao Shilpa , Gonzalez Manuel. L. , Stevens Todd M. , Lin Diana , Carroll William , Greene Benjamin , Moore Lindsay S. , Fuson Andrew , James Sherin , Hartman Yolanda E. , McCammon Susan , Panuganti Bharat , Nabell Lisle M. , Li Yufeng , Li Mei , Bailey Luke , Rosenthal Eben L. , Jeyarajan Harishanker , Thomas Carissa M. , Warram Jason M.
Published: September 2024

AbstractPurpose:
The incidence of oropharyngeal squamous cell carcinoma (OPSCC) has continually increased during the past several decades. Using transoral robotic surgery (TORS) significantly improves functional outcomes relative to open surgery for OPSCC. However, TORS limits tactile feedback, which is often the most important element of cancer surgery. Fluorescence-guided surgery (FGS) strategies to aid surgeon assessment of malignancy for resection are in various phases of clinical research but exhibit the greatest potential impact for improving patient care when the surgeon receives limited tactile feedback, such as during TORS. Here, we assessed the feasibility of intraoperative fluorescence imaging using panitumumab-IRDye800CW (PAN800) during TORS in patients with OPSCC.
Patients and Methods:
Twelve consecutive patients with OPSCC were enrolled as part of a nonrandomized, prospective, phase II FGS clinical trial using PAN800. TORS was performed with an integrated robot camera for surgeon assessment of fluorescence. Intraoperative and ex vivo fluorescence signals in tumors and normal tissue were quantified and correlated with histopathology.
Results:
Intraoperative robot fluorescence views delineated OPSCC from normal tissue throughout the TORS procedure (10.7 mean tumor-to-background ratio), including in tumors with low expression of the molecular target. Tumor-specific fluorescence was consistent with surgeon-defined tumor borders requiring resection. Intraoperative robot fluorescence imaging revealed an OPSCC fragment initially overlooked during TORS based on brightfield views, further substantiating the clinical benefit of this FGS approach.
Conclusions:
The results from this patient with OPSCC cohort support further clinical assessment of FGS during TORS to aid resection of solid tumors.
Data from HER2DX Genomic Assay in HER2-Positive Early Breast Cancer Treated with Trastuzumab and Pertuzumab: A Correlative Analysis from the PHERGain Phase II Trial
Authors: Llombart-Cussac Antonio , Pérez-García Jose , Brasó-Maristany Fara , Paré Laia , Villacampa Guillermo , Gion Maria , Schmid Peter , Colleoni Marco , Borrego Manuel R. , Galván Patricia , Parker Joel S. , Buckingham Wesley , Perou Charles M. , Villagrasa Patricia , Guerrero Jose A. , Sampayo-Cordero Miguel , Mancino Mario , Prat Aleix , Cortés Javier
Published: September 2024

AbstractPurpose:
The purpose of this study was to assess the predictive capability of HER2DX assay following (neo)adjuvant trastuzumab–pertuzumab (HP)–based therapy in HER2-positive (HER2+) early breast cancer.
Experimental Design:
HER2DX was analyzed in baseline pretreatment tumors from the PHERGain trial. Patients with stage I–IIIA HER2+ early breast cancer were randomized to group A [docetaxel, carboplatin, and HP (TCHP)] and group B (HP ± endocrine therapy). PET response was evaluated after two cycles. Group A received TCHP for six cycles regardless of PET response. Group B continued with HP ± endocrine therapy for six cycles (PET responders) or with TCHP for six cycles (PET nonresponders). The primary objective of this retrospective study was to associate the HER2DX pathologic complete response (pCR) score with pCR. The secondary objective was the association of the HER2DX risk score with 3-year invasive disease-free survival (iDFS).
Results:
HER2DX was performed on 292 (82.0%) tumors. The overall pCR rate was 38.0%, with pCR rates of 56.4% in group A and 33.8% in group B. In multivariable analysis including treatment and clinicopathologic factors, the HER2DX pCR score (continuous variable) significantly correlated with pCR [OR, 1.29; 95% confidence interval (CI), 1.10–1.54; P < 0.001]. HER2DX-defined pCR-high, -med, and -low groups exhibited pCR rates of 50.4%, 35.8%, and 23.2%, respectively (pCR-high vs. pCR-low OR, 3.27; 95% CI, 1.54–7.09; P < 0.001). In patients with residual disease, the HER2DX high-risk group demonstrated numerically worse 3-year iDFS than the low-risk group (89.8% vs. 100%; HR, 2.70; 95% CI, 0.60–12.18; P = 0.197).
Conclusions:
HER2DX predicts pCR in the context of neoadjuvant HP-based therapy, regardless of chemotherapy addition, and might identify patients at higher risk of recurrence among patients with residual disease.
Plasma ctDNA as a Treatment Response Biomarker in Metastatic Cancers: Evaluation by the RECIST Working Group
Authors: Wyatt Alexander W. , Litiere Saskia , Bidard Francois-Clément , Cabel Luc , Dyrskjøt Lars , Karlovich Chris A. , Pantel Klaus , Petrie Joan , Philip Reena , Andrews Hillary S. , Vellanki Paz J. , Tolmeijer Sofie H. , Villalobos Alberu Xenia , Alfano Christian , Bogaerts Jan , Calvo Emiliano , Chen Alice P. , Toledo Rodrigo A. , de Vries Elisabeth G.E. , Seymour Lesley , Laurie Scott A. , Garralda Elena ,   for the RECIST ctDNA workshop group
Published: September 2024

Early indicators of metastatic cancer response to therapy are important for evaluating new drugs and stopping ineffective treatment. The RECIST guidelines based on repeat cancer imaging are widely adopted in clinical trials, are used to identify active regimens that may change practice, and contribute to regulatory approvals. However, these criteria do not provide insight before 6 to 12 weeks of treatment and typically require that patients have measurable disease. Recent data suggest that measuring on-treatment changes in the amount or proportion of ctDNA in peripheral blood plasma may accurately identify responding and nonresponding cancers at earlier time points. Over the past year, the RECIST working group has evaluated current evidence for plasma ctDNA kinetics as a treatment response biomarker in metastatic cancers and early endpoint in clinical trials to identify areas of focus for future research and validation. Here, we outline the requirement for large standardized trial datasets, greater scrutiny of optimal ctDNA collection time points and assay thresholds, and consideration of regulatory body guidelines and patient opinions. In particular, clinically meaningful changes in plasma ctDNA abundance are likely to differ by cancer type and therapy class and must be assessed before ctDNA can be considered a potential pan-cancer response evaluation biomarker. Despite the need for additional data, minimally invasive on-treatment ctDNA measurements hold promise to build upon existing response assessments such as RECIST and offer opportunities for developing novel early endpoints for modern clinical trials.
The Dark Knight: Functional Reprogramming of Neutrophils in the Pathogenesis of Colitis-Associated Cancer
Authors: Ghosh Sreya , Zanoni Ivan
Published: September 2024

Neutrophils are the primary myeloid cells that are recruited to inflamed tissues, and they are key players during colitis, being also present within the tumor microenvironment during the initiation and growth of colon cancer. Neutrophils fundamentally serve to protect the host against microorganism invasion, but during cancer development, they can become protumoral and lead to tumor initiation, growth, and eventually, metastasis—hence, playing a dichotomic role for the host. Protumoral neutrophils in cancer patients can be immunosuppressive and serve as markers for disease progression but their characteristics are not fully defined. In this review, we explore the current knowledge on how neutrophils in the gut fluctuate between an inflammatory or immunosuppressive state and how they contribute to tumor development. We describe neutrophils’ antitumoral and protumoral effects during inflammatory bowel diseases and highlight their capacity to provoke the advent of inflammation-driven colorectal cancer. We present the functional ambivalence of the neutrophil populations within the colon tumor microenvironment, which can be potentially exploited to establish therapies that will prevent, or even reverse, inflammation-dependent colon cancer incidence in high-risk patients.
Inflammation Mediated by Gut Microbiome Alterations Promotes Lung Cancer Development and an Immunosuppressed Tumor Microenvironment
Authors: Rahal Zahraa , Liu Yuejiang , Peng Fuduan , Yang Sujuan , Jamal Mohamed A. , Sharma Manvi , Moreno Hannah , Damania Ashish V. , Wong Matthew C. , Ross Matthew C. , Sinjab Ansam , Zhou Tieling , Chen Minyue , Tarifa Reischle Inti , Feng Jiping , Chukwuocha Chidera , Tang Elizabeth , Abaya Camille , Lim Jamie K. , Leung Cheuk Hong , Lin Heather Y. , Deboever Nathaniel , Lee Jack J. , Sepesi Boris , Gibbons Don L. , Wargo Jennifer A. , Fujimoto Junya , Wang Linghua , Petrosino Joseph F. , Ajami Nadim J. , Jenq Robert R. , Moghaddam Seyed Javad , Cascone Tina , Hoffman Kristi , Kadara Humam
Published: September 2024

Accumulating evidence indicates that the gut microbiome influences cancer progression and therapy. We recently showed that progressive changes in gut microbial diversity and composition are closely coupled with tobacco-associated lung adenocarcinoma in a human-relevant mouse model. Furthermore, we demonstrated that the loss of the antimicrobial protein Lcn2 in these mice exacerbates protumor inflammatory phenotypes while further reducing microbial diversity. Yet, how gut microbiome alterations impinge on lung adenocarcinoma development remains poorly understood. In this study, we investigated the role of gut microbiome changes in lung adenocarcinoma development using fecal microbiota transfer and delineated a pathway by which gut microbiome alterations incurred by loss of Lcn2 fostered the proliferation of proinflammatory bacteria of the genus Alistipes, triggering gut inflammation. This inflammation propagated systemically, exerting immunosuppression within the tumor microenvironment, augmenting tumor growth through an IL6-dependent mechanism and dampening response to immunotherapy. Corroborating our preclinical findings, we found that patients with lung adenocarcinoma with a higher relative abundance of Alistipes species in the gut showed diminished response to neoadjuvant immunotherapy. These insights reveal the role of microbiome-induced inflammation in lung adenocarcinoma and present new potential targets for interception and therapy.
Supplementary Figure 4 from Exploration of Immune-Modulatory Effects of Amivantamab in Combination with Pembrolizumab in Lung and Head and Neck Squamous Cell Carcinoma
Authors: Lim Sun M. , Kang Seong-san , Kim Dong K. , Lee Soo-Hwan , Synn Chun-Bong , Baek Sujeong , Yang Seung M. , Han Yu J. , Kim Mi H. , Han Heekyung , Na Kwangmin , Kim Young T. , Yun Mi R. , Kim Jae H. , Byeon Youngseon , Kim Young S. , Lee Jii B. , Hong Min H. , Curtin Joshua C. , Patel Bharvin , Bergiers Isabelle , Pyo Kyoung-Ho , Cho Byoung C.
Published: September 2024

This figure shows the single cell RNA sequencing analysis of EGFR high tumor subcluster in the humanized LUSC PDX model.
Figure S1 from Tumor-Immune Signatures of Treatment Resistance to Brentuximab Vedotin with Ipilimumab and/or Nivolumab in Hodgkin Lymphoma
Authors: Gonzalez-Kozlova Edgar , Huang Hsin-Hui , Jagede Opeyemi A. , Tuballes Kevin , Del Valle Diane M. , Kelly Geoffrey , Patel Manishkumar , Xie Hui , Harris Jocelyn , Argueta Kimberly , Nie Kai , Barcessat Vanessa , Moravec Radim , Altreuter Jennifer , Duose Dzifa Y. , Kahl Brad S. , Ansell Stephen M. , Yu Joyce , Cerami Ethan , Lindsay James R. , Wistuba Ignacio I. , Kim-Schulze Seunghee , Diefenbach Catherine S. , Gnjatic Sacha
Published: September 2024

Supplemental Figure 1. A. Volcano plots showing differentially expressed proteins with P<0.05 between treatments.
Supplementary Figure 5 from Stratification to Neoadjuvant Radiotherapy in Rectal Cancer by Regimen and Transcriptional Signatures
Authors: Mahmood Umair , Blake Andrew , Rathee Sanjay , Samuel Leslie , Murray Graeme , Sebag-Montefiore David , Gollins Simon , West Nicholas P. , Begum Rubina , Bach Simon P. , Richman Susan D. , Quirke Phil , Redmond Keara L. , Salto-Tellez Manuel , Koelzer Viktor H. , Leedham Simon J. , Tomlinson Ian , Dunne Philip D. , Buffa Francesca M. , Maughan Tim S. , Domingo Enric
Published: September 2024

Supplementary Figure 5
Clinical Effects and Safety of Intra-Arterial Infusion Chemotherapy with Lipiodol versus Intra-Arterial Infusion Chemotherapy Alone for Treatment of Advanced Hepatocellular Carcinoma
Authors: Kim Su Ho , Oh Jung Suk , Jeon Chang Ho , Chun Ho Jong , Choi Byung Gil
Published: September 2024

Introduction: This study aimed to assess the effectiveness and safety of hepatic arterial infusion chemotherapy (HAIC) in 2 groups of patients: those who receive lipiodol (referred to as the lipiodol group) and those who do not receive lipiodol (referred to as the control group). Methods: From January 2016 through December 2023, 85 patients with advanced hepatocellular carcinoma were enrolled in this retrospective study. In total, 40 patients received HAIC with lipiodol, while 45 patients were given HAIC without lipiodol. The modified response evaluation criteria for solid tumors were used to evaluate the tumor response, which was assessed through an imaging study. The two groups were compared regarding their overall survival (OS), progression-free survival (PFS), and safety. Results: The outcomes between the lipiodol group and control group demonstrated no significant difference: the objective response rates (p = 0.066) were 32.5% and 15.6%; the disease control rates (p = 0.556) were 67.5% and 73.3%; the median OS times (p = 0.339) were 224 days and 398 days; the median PFS (p = 0.334) times were 191 days and 286 days in the lipiodol group and the control group, respectively. Adverse events also showed no significant difference between the two groups: elevation of total bilirubin (p = 0.834) rates were 40.0% and 37.8%; elevation of alanine aminotransferase (p = 0.191) percentages were 35.0% and 22.2%; and elevation of aspartate aminotransferase values (p = 0.058) were 65.0% and 44.4% in the lipiodol group and the control group, respectively. Conclusions: HAIC without lipiodol was non-inferior to HAIC with lipiodol in the clinical outcome.
Relevance of Continuous EEG versus Routine EEG for Outcome Prediction after Traumatic Brain Injury
Authors: Loser Valentin , Rossetti Andrea O. , Rasic Marija , Novy Jan , Schindler Kaspar A. , Rüegg Stephan , Alvarez Vincent , Beuchat Isabelle
Published: September 2024

Introduction: In a cohort of adult patients with disturbance of consciousness after TBI, we aimed to explore the relationship of continuous video-EEG (cEEG) versus routine EEG (rEEG) with mortality and functional outcome. Methods: This is a post hoc analysis of a randomized controlled trial (CERTA), in which adults with disorder of consciousness and needing EEG (excluding those with proven seizures/SE just before) were randomized 1:1 to cEEG or two rEEG. In TBI patients, correlation between EEG duration, mortality, and modified Rankin score (mRs, good 0–2) at 6 months was assessed. Results: Among 364 patients, 44 presenting with consciousness impairment after TBI were included; 29 randomized to cEEG and 15 to rEEG. Mortality (p = 0.88) and functional outcome (p = 0.58) at 6 months were similar between groups. There was a nonsignificant tendency toward more seizure/status epilepticus detection with cEEG (p = 0.08). In multivariable regression, cEEG was not related to functional outcome (OR: 0.75 [0.13–4.24], p = 0.745) or mortality (OR: 7.11 [0.51–99.32], p = 0.145). Conclusion: Despite allowing increased seizure detections in TBI patients, cEEG does not seem to be associated with better functional outcome or mortality over rEEG. Pending larger trials, repeated rEEG might be acceptable in post-TBI disorder of consciousness, especially in resource-limited environments.
Bites and Stings: Exotic Causes of Stroke in Asia
Authors: Ranawaka Udaya K.
Published: September 2024

Background: Many unusual and fascinating causes of stroke are seen in Asia. Although rarely encountered in the Western world, such cases are seen not so infrequently by neurologists, physicians, and other healthcare workers in Asian countries. This article highlights some of these intriguing causes of stroke such as snakebite, scorpion stings, and bee and wasp stings. Summary: Snakebite is a neglected tropical disease of global importance affecting over 5 million people each year, and scorpion stings and bee and wasp stings affect over a million people worldwide every year. Strokes due to these uncommon causes predominantly affect young males in their most productive years, and many victims die or remain with residual disability with significant socioeconomic consequences to the affected families and society. Both ischaemic and haemorrhagic strokes are seen, with ischaemic strokes being more common. Many unanswered questions remain regarding the pathophysiological mechanisms, clinical spectrum, and the natural history of stroke due to these causes. Antivenom is effective in snake envenoming, antivenom and prazosin are effective in scorpion envenoming, and treatment of anaphylaxis and allergy is recommended in systemic reactions due to bee or wasp stings. However, there are no treatment guidelines or expert recommendations to guide the management of stroke in these patients, and there are no data on the safety and efficacy of stroke-specific treatments such as antiplatelet therapy, thrombolysis or endovascular treatment. Key Messages: More research is clearly needed to unravel the complexities related to stroke following snakebite, scorpion envenoming, and bee and wasp stings, and to guide the development of optimal stroke treatment strategies in these unusual situations. Awareness of these exotic stroke aetiologies is important in early recognition and appropriate management of patients presenting with stroke symptoms in Asian countries, and in global travellers from the region. International collaborations would go a long way in improving our understanding of these rare but fascinating causes of stroke.
A scoping review of economic evaluations of post-traumatic stress disorder patients’ intervention
Authors: Dwarika Veronica , Amedu Amos Nnaemeka
Published: September 2024

Intervention programmes for patients with post-traumatic stress disorder (PTSD) have been extensively evaluated economically, and the related implementation costs are presumably high. This study reviewed the cost-effectiveness of PTSD intervention programmes and associated health benefits. The literature search was carried out between June and July 2023. The PRISMA guidelines for scoping review protocols were strictly followed. A literature search was conducted on the National Health Service (NHS) Economic Evaluation Database, PubMed, PsycInfo, and EconLit, this search yielded 16 studies. This review revealed that trauma-focused cognitive-behavioural therapy (+selective serotonin re-uptake inhibitor) (TF+CBT+SSRIs); prolonged exposure therapy; MDMA-assisted therapy (MDMA-AT); stepped care, screen and treatment; and the transmural trauma care model were cost-effective for PTSD treatment. The cost-effectiveness of intervention programmes that lasted 10 to 31 years was greater. In general, long-term intervention programmes have been associated with health benefits. It is also important to note that telemedicine, cognitive-behavioural therapy, biopsychosocial therapy, and individual therapeutic interventions are capital-intensive and inefficient. When disbursing funding for PTSD treatment around the world, the world health organisation (WHO) should keep in mind these identified interventions and the countries where they have been found to be cost-effective.
The effectiveness of using ID broth in identifying the outer membrane protein of Salmonella typhi
Authors: Pestariati Pestariati , Suhariyadi Suhariyadi
Published: September 2024

Typhoid fever is an infection that affects the digestive system. It spreads through contaminated food and drinks due to the Salmonella bacteria. One way to develop immunity against Salmonella typhi is by using outer membrane protein (OMP), which activates the cellular immune system. This research aimed to determine the effectiveness of using ID Broth to identify OMP Salmonella typhi. The study was conducted experimentally at the Institute of Tropical Disease from April 2023 to May 2023. For the study, we obtained three samples of Salmonella typhi isolated from East Java, and each sample was replicated three times. We isolated the bacteria and extracted the OMP to measure its levels and perform electrophoresis with SDS-PAGE. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a standard protein analysis method. To address sample loading challenges due to stacking gel transparency, an acidic dye was added to improve visibility without affecting gel performance. In this study nutrient agar from MacConkey medium and ID broth were used as variables. We cultured Salmonella typhi and extracted OMP using the sonication technique. We measured protein levels through the nanodrop method. Salmonella typhi from ID broth produced higher protein levels than Salmonella typhi cultured from MacConkey Medium. It affected the identification of OMP using SDS-PAGE. Lower protein levels lead to fewer protein molecules in the same band zone, causing reduced visibility and readability of the protein bands. The ID broth stabilizes the bacteria's condition before being grown on nutrient agar media.
“Pineapple eyes”: local social networks to prevent and control epidemics in Thailand’s southern beach tourism provinces
Authors: Jitwarin Lapasrada , Chinabhark Krittabhart , Jitwarin Tawatchai
Published: September 2024

The study aimed to qualitatively examine epidemic prevention and control processes in beach tourism provinces in the upper southern region of Thailand, as well as investigate networks related to public health emergency management (PHEM) during such epidemics. The findings highlighted key elements of PHEM for emerging infectious diseases. This involved initial assessments of epidemics and health conditions, categorizing the population by risk, and performing prognosis. Subsequently, operational guidelines for prevention, disease control, and treatment were proposed and often submitted for consideration and orders to the provincial communicable disease control committee. Meetings were held to disseminate committee announcements and measures for implementation by relevant departments, enhancing operational guideline development. Successful local networks as pineapple eyes for PHEM in response to emerging infectious diseases were characterized by robust social networks and informal cooperation at village and community levels, serving as crucial mechanisms for disease control and care. Additionally, business sector networks transformed "mutual suffering" into cooperation, with entrepreneurs in the tourism industry taking the lead. They developed various websites and applications at different stages to promote and support situation management, proving to be a vital mechanism for an effective response to emerging infectious diseases.
The experience of COVID-19: assessing the Malaysian counselors in training coping strategies
Authors: Abdullah Siti Salina , Arifin Suhaili , Ramley Fazliyaton , Md Shah Kamarul , Yusop Yusni Mohamad , Ahamad Masturina , Mat Najib Norazizalina
Published: September 2024

The COVID-19 pandemic also had a psychological impact on various levels of society including trainee counselors. Therefore, the aim of this study is to explore the coping strategies employed by trainee counselors to overcome their challenges within their internship during COVID-19. This study is a quantitative study and was conducted among 106 final year trainee counsellors enrolled in a bachelor's counselling program at the University Malaysia Terengganu (UMT). There were two instruments used in this study, consisting of a sociodemographic questionnaire and the Malay Brief COPE (MBC) scale. The findings indicate that emotion focused is mostly used by the counsellors in training with the mean value of 28.96 (SD=4.891); the mean of problem-focused was 18.08 (SD=3.080); and the mean of avoidance coping was 26.12 (SD=5.341). The study's dataset can be used by counselor educators, the Malaysian Board of Counsellors, clinicians, and academicians to improve the effectiveness and competencies of trainee counsellors completing internship during the adverse period.
SEaM mobile card game and animated video for increasing knowledge and attitudes of early marriage prevention
Authors: Susmita Sari Apriani , Lia Basuni Hikmah , Fitria Aprianti Nurannisa
Published: September 2024

An international problem that might jeopardize the lives, futures, and well-being of adolescents is early marriage. A couple who marries before the age of eighteen is considered an early marriage. Knowledge and attitudes towards early marriage are among the variables that can impact its incidence. This study aimed to determine the effect of using the SEaM Mobile Card Game and animated videos on increasing the knowledge and attitudes of adolescents about early marriage prevention. The research design is a quasi-experiment with a non-equivalent approach (pre-test and post-test) control group design. The sample amounted to 148 respondents. The instruments used in this study were knowledge questionnaires on early marriage prevention, attitude questionnaires on early marriage prevention and usefulness, ease of use, ease of learning, and satisfaction (USE) questionnaires. The research data were analyzed using Wilcoxon and Mann Whitney with a significance level of p<0.05. The results of this study were that there was a significant difference in adolescent knowledge about early marriage prevention in the SEaM Mobile Card Game group and animated videos after education (p=0.013) and there was a significant difference in adolescents' attitudes toward early marriage prevention in the SEaM Mobile Card Game group and animated videos after education (p=0.000).
Examining pycho-social wellbeing among factory workers
Authors: Kumar Shankar , Reddy Nellore Pratika
Published: September 2024

In India, factory employees make up a sizeable portion of the labour force and are essential to the country's industrial sector. Working in a factory may be mentally and physically demanding, which can lead to stress, burnout, and other mental health issues. The researcher in the study focused on the impact of mental wellbeing on job performance and examining the mediating effect of job satisfaction and moderating effect of social competence among factory workers. The study's target population is factory workers working in the locality of Tamil Nadu with a sample size of 114 workers. A descriptive study design with a quantitative approach and non-probability convenience sampling is applied. The hypotheses formulated were tested by using Partial Least Squares Structural Equation Modeling (PLS-SEM) through SmartPLS. It is evident from the study there is a significant impact on mental wellbeing on job performance; mental wellbeing on job satisfaction and job satisfaction on job performance. Prioritising mental health in the workplace shows a dedication to the all-encompassing care of employees and promotes a positive organisational culture that values employee well-being. These factors will positively affect employee health and happiness, productivity, absenteeism rates, turnover rates, workplace safety, and ethical responsibility. Future study can concentrate on mindfulness training, stress management initiatives, counselling services on performance results.
Father involvement as a predictor of early childhood external behaviors in Indonesian
Authors: Nafisah Aisyah Durrotun , Sugiyo Pranoto Yuli Kurniawati , Nuzulia Siti , Mulawarman Mulawarman , Suminar Tri
Published: September 2024

The prevailing patriarchal culture in Indonesia that posits a father only to act as a breadwinner is thought to be the reason for the lack of father involvement in parenting. Therefore, this study aims to analyze father involvement in problematic behavior in children aged 3-4 years by considering the variables of father's socioeconomic status (SES) and child's gender. This study employs a quantitative descriptive approach through a multiple regression model coupled with survey. The selection of respondents was determined through quota sampling technique by considering the ease of access to data collection. With or without control over the SES of fathers and the gender of children, the engagement indicator contributes an impact to the problematic behavior in chidren, while accessibility does not. The problematic behavior in children is lower with the control over SES of fathers and the gender of children compared to the condition without control over these two variables. Of the two variables, only the father’s age indicator affects the problematic behavior in children. The results of this study can encourage fathers to get actively involved in raising their children in a way that is appropriate to the current cultural context and situation.
Health consideration in food consumption: impacts of education level and custom rules adherence
Authors: Sari Ni Putu Wulan Purnama , Mazenda Adrino , Tristiana RR Dian
Published: September 2024

Individual attributes, such as educational background, may influence the degree of health consideration in food consumption. The local social norms may affect the same consideration in the collective level. Represented by education level and the custom rules adherence in food choosing behavior, this study aimed to examine how knowledge influences health consideration in food consumption and how the local social norms moderate this association in a multicultural enriched society. By utilizing the Bayesian Mindsponge Framework (BMF) as a conceptual framework, this study analyzed a dataset of 710 urban residents in Indonesia. There was a negative association between education level and health consideration in food consumption in which the custom rules adherence in food choosing behavior moderated against this negative association. For those with a low level of custom-based food consumption, their health consideration is lower as they have higher education level. However, the opposite trend which is a more intuitive positive association was found in those who consume more custom-based food. Socio-cultural factors have a complex impact on food choosing behavior among Indonesian urban residents. Therefore, governmental policies in the health promotion campaign of healthy-nutritious food should incorporate socio-cultural aspects with a deeper understanding of the human mind’s information processing.
The economic impact of COVID-19 pandemic on physicians/health care work force in Egypt
Authors: Ahmed Rehab Abdelhai , Salem Amany Ahmed , Elsebaie Eman Hany , Shamseldein Sherif , Hassan Mustafa Ahmed , Sedrak Amal Samir
Published: September 2024

The COVID-19 pandemic has impacted healthcare workers physically, psychologically, and economically. The industry has struggled financially with the cancellation of elective procedures and hesitancy from patients to seek medical help. Thus, this study aimed to assess the economic and work burden of COVID-19 on the health workforce in Egypt. Methods a cross-sectional survey was conducted in Egypt from October 2020 to May 2021. A purposive sample of 763 healthcare workers (HCWs) from Egyptian governorates was included. HCWs were asked to fill out a self-administered questionnaire on Google Forms and printed copies. The questionnaire link was shared on social media forums. Results a total of 763 HCWs responded. Females were 88.2% and more than half were 33 or less years old. Physicians constituted 42.5%, while paramedics 57.5%. The governmental sector represented 61.2%, private sector 14.7% and those who combined both 24.1%. Regarding specialties affected, pediatricians, dermatologists, and nephrologists reported a considerable drop in their regular private work rate, while radiologists were not affected regarding workload or income by the closure. The HCWs acknowledge that COVID-19 has put extreme stress on the healthcare workforce physically, mentally, and financially in a pre-existing challenging environment. The results can help evidence-based decisions by policymakers in Egypt.
The effectiveness of progressive muscle relaxation for air traffic controller in Indonesia
Authors: Saleh Lalu Muhammad , Russeng Syamsiar S. , Tadjuddin Istiana , Yanti Iva Hardi , Syafitri Nurul Mawaddah , Rahmadani Yulianah , Yusbud Mahfuddin
Published: September 2024

Evaluation of progressive muscle relaxation (PMR) intervention testing is deemed necessary for air traffic controllers as their role in controlling aircraft airways requires high focus with stable physical and mental conditions. This study aimed to analyze the effectiveness of progressive muscle relaxation in improving health status, quality of life, and reducing psychological fatigue in air traffic controllers. A quasi-experimental pre-test post-test control group design was employed, involving 184 participants separated into intervention and control groups. The results showed that there was a significant difference in the pulse rate, oxygen level, specific diastolic blood pressure, depression, anxiety, quality of life domains 1 and 2, as well as feelings of fatigue before and after progressive muscle relaxation was implemented with p<0.05 but not in control group at the end of the observation. The Mann-Whitney U-Test results indicated that anxiety and pulse rate are variables reduced significantly in the two groups. It can be concluded that the implementation of PMR is effective to improve the health status, quality of life, and reduce psychological fatigue among controller in Indonesia. 
Informing compliance factors regarding hospitals waste management procedures in Indonesia: a scoping review
Authors: Kaban Markus , Soeprobowati Tri Retnaningsih , Suhartono Suhartono , Pireno Purwanto Adi , Kristanti Alberta Widya , Sebong Perigrinus Hermin
Published: September 2024

The hospital staff's non-compliance in managing hazardous waste can hurt the environment and public health. There are various practicals to mitigate the harmful effects of hazardous medical waste on the environment and public health. However, there needs to be evidence to provide accurate data regarding compliance with hazardous waste management at hospitals in Indonesia. This study aims to assess and map the determinants affecting hospital liquid medical waste management. This study examined electronic databases from 2017-2023. Studies were screened based on inclusion and exclusion criteria. The study was grounded in the scoping review to inform compliance factors regarding waste management in Hospitals. One article describes compliance with the regulation, and the other concerns supervising hospital waste management. Those articles are not detailed enough in describing supervision, so we suggest further research about supervision support to foster the management process and the result of hospital waste management. Regulation is essential for all processes and products that affect the environment. Compliance with guidelines needs to become a habit and improve over time.
Computer program in sign language for controlling mobile objects and communicating with people
Authors: Moldamurat Khuralay , Tulembayeva Aigul , Ryspaev Askhat , Belgibekov Niyaz , Peryakina Lyudmila , Bakyt Makhabbat
Published: September 2024

This article emphasizes the need for effective communication methods for people with disabilities, focusing on gestures and finger movements. The study aims to address the communication support gap for this group by creating a specialized Kazakh IT dictionary. It explores practical ways to facilitate communication for people with disabilities through gestures, utilizing computer software for the IT dictionary. The article presents key variables, study design, and data analysis procedures. Results show computer programs effectively enable communication for individuals with disabilities. The study's novelty lies in the IT vocabulary covering gestures, benefiting pedagogy, education, and related fields. Computational linguistics, like automatic language processing and machine translation, enhances communication facilitation for the disabled. Practical recommendations are offered for implementing research findings, improving communication and overall quality of life for people with disabilities. In conclusion, the article stresses the importance of effective communication methods, especially gestures, for the disabled. The IT vocabulary creation through computational linguistics offers innovative ways to enhance communication accessibility, prompting further research.
Emotional intelligence trends in nursing education: review the literature
Authors: Matroud Fouziyah , Othman Mohd Syaubari , Mohd Jamil Mohd Ridhuan , Hosshan Hasrul , Md Zabit Mohd Nazir , Yahya Faridah Hanim , Azhari Azman Mohamed Nor , Setambah Mohd Afifi Bahurudin
Published: September 2024

The high demand for nursing work to encounter death or disease, dealing with a high volume of work in a short amount of time, working under intense pressure, and ignoring established authority all contribute to the high levels of stress on nurses. The undisputable significance of emotional intelligence in the sphere of nursing education has led in a rapid increase in the number of emotional intelligence courses and training program that offer a broad range of educational different outcomes to the students. There is a lack of studies regarding to improve teaching and learning in Libyan nursing educational institutions. Quite a few emotional intelligence (EI) assessment studies were found that simply contain criteria and dimensions of EI in the literature study. The aim of this study is to review the emotional intelligence studies in nursing education. This evaluation encompasses a range of publications released throughout the time frame of 2019 to 2023. The findings showed that there is a need for including emotional intelligence program in curriculum of nursing education. In addition, the findings indicated that EI intervention program could boost student academic achievements and alleviate work stress to nurse profession. The discussion chiefly emphasizes on the contemporary issues that are being confronted by the field, as well as the openings for research that exist in the area.
Anaemia: female youth’s knowledge and supplementation program management
Authors: Khomsan Ali , Riyadi Hadi , Prasetya Guntari
Published: September 2024

The prevalence of anaemia among adolescent girls in Indonesia is 18.4%. One of the main indicator targets is reducing the prevalence of anaemia is in the form of iron-folic acid (IFA) supplementation for adolescent girls. The research aims to analyze knowledge of female youth on anaemia and management of iron-folic acid supplementation. This study used a cross-sectional design, and it was conducted in 24 senior high schools in Cianjur Regency (high stunting area) and Depok City (low stunting area). The total sample of this study was 243 female youth. The study began from September 2019 to February 2020 for the 1st term and continued from October 2022 to March 2023 for the 2nd term. The quantitative result showed that there was a significant difference in knowledge about the frequency of providing the iron-folic acid supplement (IFAS) at school and the duration in a year that students must consume the IFAS between high stunting and low stunting (p=0.000). A significant proportion of female youth, 67.5% in high stunting areas and 71.7% in low stunting areas, exhibit insufficient nutrition knowledge, underscoring the necessity for a nutrition education initiative targeting high school students. The qualitative results showed the input-process-output of the IFA program have been administered for the last few years, providing several constraints in order to improve the IFA program management. Based on those findings, the IFA supplementation program provides a potential chance for addressing anaemia in female youth.
Efficacy of calcium lactate as a biodegradable coagulant for peat water purification: an experiment comparative study
Authors: Asmadi Asmadi , Akhmadi Zainal , Suharno Suharno , Yulia Yulia
Published: September 2024

Kalimantan relies significantly on peat water as a water source. However, due to its high organic content, acidic nature, and intense color, it is not safe to drink. To address this issue, researchers proposed an eco-friendly coagulant, calcium lactate, to remove suspended solids. To test its effectiveness, the coagulant was compared to membrane technologies, specifically nanofiltration (NF) and reverse osmosis (RO), at Rasau Jaya General Hospital in West Kalimantan between April and October 2022. Different pressures, 40, 60, and 80 Psi, were used to test both methods. Before and after treatment, the levels of total coliform, E. coli, pH, total dissolved solid (TDS), turbidity, color, and calcium were examined. The results showed that the best outcomes were achieved at 60 Psi. The combination of 0.8 mg/l calcium lactate and NF met all the standard values, except for color (29 TCU). Calcium lactate and RO, however, produced safe drinking water at 60 Psi from all analyzed parameters (E. coli=0, total coliform=0, pH=6.5, TDS=7.1, nephelometric turbidity units (NTU)=2.8 NTU, color=8.7 TCU, and calcium 7.6 mg/l). The reduction of bacteriological substances achieved 100% for both methods. Overall, the combination of calcium lactate and RO yielded slightly better results than NF.