bluuebunny/crossref_metadata_2025 huggingface dataset:
Chinese medical students’ disposition for critical thinking: a mixed methods exploration
Authors: Huang Lei , Fan Angela Pei-Chen , Su Na , Thai Jessica , Kosik Russell Olive , Zhao Xudong
Published: July 2021

Background

Critical thinking (CT) is an essential competency for medical students. This study’s aim was to evaluate Chinese medical students’ disposition for CT and to explore the impact of current trends in medical education on students’ CT development.

Methods

We used multistage stratified cluster sampling to recruit a total of 1241 medical students among five different years of training and from three medical institutions in China. The Critical Thinking Disposition Inventory-Chinese Version (CTDI-CV) and self-reported information were used to collect cross-sectional data. Based on the data from the CTDI-CV, 112 medical students in clinical course training from a single institution continued one-year follow-up. Their one-year CTDI-CV score changes were collected regarding various medical education variables.

Results

The mean CTDI-CV score of the 1241 medical students was 287.04 with 729 (58.7%) students receiving a score of 280 or higher. There were statistically significant differences in schools attended(F = 3.84, P < 0.05), year of school attended(F = 10.32, P < 0.001), GPA(F = 6.32, P < 0.01), weekly time spent learning after class(F = 14.14, P < 0.001), attitude toward medicine(F = 28.93, P < 0.001), desire to be a doctor after graduation(t = − 3.35, P < 0.001), familiarity with CT(F = 20.40, P < 0.001), and perception of importance of CT(F = 22.25, P < 0.001). The participants scored the highest on the CTDI-CV subscales of “inquisitiveness” and the lowest on “truth seeking.” The 112 students in the longitudinal study had significantly lower total CT scores after one academic year follow-up.

Conclusions

Chinese medical students generally exhibited positive CT dispositions. The cross-sectional survey and one-year longitudinal study indicated that students’ CT disposition diminished as they progressed through traditional medical training. Our study contributes to understanding the status of Chinese medical education of and influential factors on medical students’ CT disposition.
Hidden labour: the skilful work of clinical audit data collection and its implications for secondary use of data via integrated health IT
Authors: McVey Lynn , Alvarado Natasha , Greenhalgh Joanne , Elshehaly Mai , Gale Chris P. , Lake Julia , Ruddle Roy A. , Dowding Dawn , Mamas Mamas , Feltbower Richard , Randell Rebecca
Published: July 2021

Background

Secondary use of data via integrated health information technology is fundamental to many healthcare policies and processes worldwide. However, repurposing data can be problematic and little research has been undertaken into the everyday practicalities of inter-system data sharing that helps explain why this is so, especially within (as opposed to between) organisations. In response, this article reports one of the most detailed empirical examinations undertaken to date of the work involved in repurposing healthcare data for National Clinical Audits.

Methods

Fifty-four semi-structured, qualitative interviews were carried out with staff in five English National Health Service hospitals about their audit work, including 20 staff involved substantively with audit data collection. In addition, ethnographic observations took place on wards, in ‘back offices’ and meetings (102 h). Findings were analysed thematically and synthesised in narratives.

Results

Although data were available within hospital applications for secondary use in some audit fields, which could, in theory, have been auto-populated, in practice staff regularly negotiated multiple, unintegrated systems to generate audit records. This work was complex and skilful, and involved cross-checking and double data entry, often using paper forms, to assure data quality and inform quality improvements.

Conclusions

If technology is to facilitate the secondary use of healthcare data, the skilled but largely hidden labour of those who collect and recontextualise those data must be recognised. Their detailed understandings of what it takes to produce high quality data in specific contexts should inform the further development of integrated systems within organisations.
The economic value of changing mortality risk in low- and middle-income countries: a systematic breakdown by cause of death
Authors: Khadka Aayush , Verguet Stéphane
Published: July 2021

Background

We develop a framework for quantifying monetary values associated with changes in disease-specific mortality risk in low- and middle-income countries to help quantify trade-offs involved in investing in mortality reduction due to one disease versus another.

Methods

We monetized the changes in mortality risk for communicable and non-communicable diseases (CD and NCD, respectively) between 2017 and 2030 for low-income, lower-middle-income, and upper-middle-income countries (LICs, LMICs, and UMICs, respectively). We modeled three mortality trajectories (“base-case”, “high-performance”, and “low-performance”) using Global Burden of Disease study forecasts and estimated disease-specific mortality risk changes relative to the base-case. We assigned monetary values to changes in mortality risk using value of a statistical life (VSL) methods and conducted multiple sensitivity analyses.

Results

In terms of NCDs, the absolute monetary value associated with changing mortality risk was highest for cardiovascular diseases in older age groups. For example, being on the low-performance trajectory relative to the base-case in 2030 was valued at $9100 (95% uncertainty range $6800; $11,400), $28,300 ($24,200; $32,400), and $30,300 ($27,200; $33,300) for females aged 70–74 years in LICs, LMICs, and UMICs, respectively. Changing the mortality rate from the base-case to the high-performance trajectory was associated with high monetary value for CDs as well, especially among younger age groups. Estimates were sensitive to assumptions made in calculating VSL.

Conclusions

Our framework provides a priority setting paradigm to best allocate investments toward the health sector and enables intersectoral comparisons of returns on investments from health interventions.
Circular RNA-DPP4 serves an oncogenic role in prostate cancer progression through regulating miR-195/cyclin D1 axis
Authors: Yang Deping , Yang Bo , Zhu Yanjun , Xia Qianlin , Zhang Yan , Zhu Xin , Guo Jianming , Ding Tao , Zheng Jianghua
Published: July 2021

Background

Recently, more and more studies have highlighted the critical regulatory roles of circular RNAs (circRNAs), a class of non-coding RNAs, in the progression of many human cancers, including prostate cancer (PCa). circRNA microarray analysis was performed to identify circRNAs that are differentially expressed in PCa tissues.

Methods

104 pairs of PCa tissues and matched adjacent normal prostate tissues (at least 2 cm distal to the tumor margin) were obtained. circRNA microarray analysis was performed on four pairs of PCa tissues and matched adjacent normal prostate tissues to investigate the potential involvement of circRNAs in PCa. Flow cytometric analysis was performed to investigate whether the effect of circDPP4 on PCa cell proliferation was associated with the alteration in cell cycle progression. The role of circDPP4 in PCa tumor growth was further explored in vivo.

Results

We found that circDPP4 was overexpressed in PCa tissues and cell lines, and its expression was closely associated with Gleason score and clinical stage of PCa patients. In vitro loss- and gain-of-function experiments demonstrated that circDPP4 knockdown inhibited, whereas circDPP4 overexpression promoted the proliferation, migration, invasion and cell cycle progression of PCa cells. Knockdown of circDPP4 also suppressed PCa tumor growth in vivo. We further found that circDPP4 functioned as a competing endogenous RNA (ceRNA) for miR-195 in PCa cells, and miR-195 negatively regulated the expression of oncogenic cyclin D1. Rescue experiments suggested that restoration of miR-195 blocked the oncogenic role of circDPP4 in PCa cells.

Conclusions

Taken together, our findings revealed a novel regulatory mechanism between circDPP4 and miR-195/cyclin D1 axis, and offered novel strategies for the treatment of PCa.
Task-shifting to optimize outpatient neurological care in Zambia
Authors: Villegas Ana C. , Saylor Deanna , Kvalsund Michelle , Atadzhanov Masharip , Chiluba Clarence , Chishimba Lorraine , Zimba Stanley , Chomba Mashina , Siddiqi Omar K.
Published: July 2021

Objective

To investigate opportunities for task shifting to decongest an outpatient neurology clinic in Zambia by describing current patient flow through the clinic and potential nodes for intervention using process mapping.

Background

Zambia has a population of approximately 18 million people with 4 full-time adult neurologists, as of 2018, who all practice at the University Teaching Hospital (UTH), the main tertiary care center in the country. As a result of this provider-to-patient ratio, the outpatient neurology clinic is overcrowded and overbooked. Task-shifting programs have shown to improve efficiency, access and quality of care through the use of less specialized healthcare workers in low- and middle-income countries (LMIC).

Methods

We evaluated patient flow in the UTH neurology outpatient clinic through the development and analysis of a process map. The characteristics of the clinic population between 2014 and 2018 were retrospectively reviewed from the clinic register. Between July and August 2018, we prospectively collected appointment lag times and time each patient spent waiting at various points in the clinic process. We conducted interviews with clinic staff and neurologists to generate a detailed process map of current pathways to care within the clinic. We then devised task-shifting strategies to help reduce patient wait times based on the overview of clinic process mapping and patient demographics.

Results

From 2014 to 2018, there were 4701 outpatients seen in the neurology clinic. The most common neurological diagnoses were epilepsy (39.2%), headache (21.5%) and cerebrovascular disease (16.7%). During prospective data collection, patients waited an average of 57.8 (SD 73.4) days to be seen by a neurologist. The average wait time from arrival in the clinic to departure was 4.0 (SD 2.5) h. The process map and interviews with clinic staff revealed long waiting times due to a paucity of providers. Nurses and clerks represent an influential stakeholder group, but are not actively involved in any activity to reduce wait times. A large proportion of follow-up patients were stable and seen solely to obtain medication refills.

Conclusions

Epilepsy, headache, and stroke make up the largest percentage of outpatient neurological illness in Zambia. Targeting stable patients in these diagnostic categories for a task-shifting intervention may lead to substantially decreased patient wait times. Potential interventions include shifting clinical follow-ups and medication refills to less specialized healthcare workers.
Building the field of food systems research: commentary on a research funder’s role
Authors: Pelletier Hayley , Bleecker Leah , Sauveplane-Stirling Victoria , Di Ruggiero Erica , Sellen Daniel
Published: July 2021

Background

The Food, Environment, and Health (FEH) program of the International Development Research Centre (IDRC) aims to improve the health of low- and middle-income country populations by generating evidence, innovations, and policies that reduce the health and economic burdens of preventable chronic and infectious diseases. A predominant focus of the FEH program is research related to consumer food environments that promote or enable healthy and sustainable shifts in consumption. An evaluation of the FEH program, led by the University of Toronto, provided an opportunity to analyse the approach and role of a development funder in building the field of food systems research.

Discussion

In this commentary, we provide an external evaluator’s perspective on the IDRC’s contributory role in building the field of food systems research, based on a secondary analysis of findings from a recent FEH program evaluation. We used the field-building framework outlined in Di Ruggiero et al. (Health Res Policy System, 2017) to highlight the strengths and challenges of the FEH’s approach to field-building and determined that the program aligns with six of the seven features of the framework. The FEH program has enhanced support and awareness for food systems research, provided organized funding and capacity-building opportunities, multilevel activity to support research and its use, and strong scientific leadership, and set significant standards and exemplars. However, we also found that not all sociopolitical environments have fully recognized or valued food systems research and its use for policy change.

Conclusion

The FEH program’s field-building approach can be situated within the field-building framework, and it has been successful in laying the groundwork for building the field of food systems, particularly consumer food environments research. However, supportive external environments and further investments may be needed to achieve a critical mass of capacity, continue building communities of practice, and influence policy. The FEH program approach may serve as an exemplar and comparator for other research funding agencies looking to develop strategic research programming in the field of food systems research.
A catalog of validity indices for step counting wearable technologies during treadmill walking: the CADENCE-Kids study
Authors: Gould Zachary R. , Mora-Gonzalez Jose , Aguiar Elroy J. , Schuna John M. , Barreira Tiago V. , Moore Christopher C. , Staudenmayer John , Tudor-Locke Catrine
Published: July 2021

Background

Wearable technologies play an important role in measuring physical activity (PA) and promoting health. Standardized validation indices (i.e., accuracy, bias, and precision) compare performance of step counting wearable technologies in young people.

Purpose

To produce a catalog of validity indices for step counting wearable technologies assessed during different treadmill speeds (slow [0.8–3.2 km/h], normal [4.0–6.4 km/h], fast [7.2–8.0 km/h]), wear locations (waist, wrist/arm, thigh, and ankle), and age groups (children, 6–12 years; adolescents, 13–17 years; young adults, 18–20 years).

Methods

One hundred seventeen individuals (13.1 ± 4.2 years, 50.4% female) participated in this cross-sectional study and completed 5-min treadmill bouts (0.8 km/h to 8.0 km/h) while wearing eight devices (Waist: Actical, ActiGraph GT3X+, NL-1000, SW-200; Wrist: ActiGraph GT3X+; Arm: SenseWear; Thigh: activPAL; Ankle: StepWatch). Directly observed steps served as the criterion measure. Accuracy (mean absolute percentage error, MAPE), bias (mean percentage error, MPE), and precision (correlation coefficient, r; standard deviation, SD; coefficient of variation, CoV) were computed.

Results

Five of the eight tested wearable technologies (i.e., Actical, waist-worn ActiGraph GT3X+, activPAL, StepWatch, and SW-200) performed at < 5% MAPE over the range of normal speeds. More generally, waist (MAPE = 4%), thigh (4%) and ankle (5%) locations displayed higher accuracy than the wrist location (23%) at normal speeds. On average, all wearable technologies displayed the lowest accuracy across slow speeds (MAPE = 50.1 ± 35.5%), and the highest accuracy across normal speeds (MAPE = 15.9 ± 21.7%). Speed and wear location had a significant effect on accuracy and bias (P < 0.001), but not on precision (P > 0.05). Age did not have any effect (P > 0.05).

Conclusions

Standardized validation indices focused on accuracy, bias, and precision were cataloged by speed, wear location, and age group to serve as important reference points when selecting and/or evaluating device performance in young people moving forward. Reduced performance can be expected at very slow walking speeds (0.8 to 3.2 km/h) for all devices. Ankle-worn and thigh-worn devices demonstrated the highest accuracy. Speed and wear location had a significant effect on accuracy and bias, but not precision.

Trial registration

Clinicaltrials.govNCT01989104. Registered November 14, 2013.
Salidroside promoted osteogenic differentiation of adipose-derived stromal cells through Wnt/β-catenin signaling pathway
Authors: Li Xiao-hua , Chen Fu-ling , Shen Hong-lin
Published: July 2021

Background

Bone disease causes short-term or long-term physical pain and disability. It is necessary to explore new drug for bone-related disease. This study aimed to explore the role and mechanism of Salidroside in promoting osteogenic differentiation of adipose-derived stromal cells (ADSCs).

Methods

ADSCs were isolated and treated with different dose of Salidroside. Cell count kit-8 (CCK-8) assay was performed to assess the cell viability of ADSCs. Then, ALP and ARS staining were conducted to assess the early and late osteogenic capacity of ADSCs, respectively.

Then, differentially expressed genes were obtained by R software. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially expressed genes were further analyzed. The expression of OCN, COL1A1, RUNX2, WNT3A, and β-catenin were measured by real-time PCR and Western blot analysis. Last, β-catenin was silenced by small interfering RNA.

Results

Salidroside significantly increased the ADSCs viability at a dose-response manner. Moreover, Salidroside enhanced osteogenic capacity of ADSCs, which are identified by enhanced ALP activity and calcium deposition. A total of 543 differentially expressed genes were identified between normal and Salidroside-treated ADSCs. Among these differentially expressed genes, 345 genes were upregulated and 198 genes were downregulated. Differentially expressed genes enriched in the Wnt/β-catenin signaling pathway. Western blot assay indicated that Salidroside enhanced the WNT3A and β-catenin expression. Silencing β-catenin partially reversed the promotion effects of Salidroside. PCR and Western blot results further confirmed these results.

Conclusion

Salidroside promoted osteogenic differentiation of ADSCs through Wnt/β-catenin signaling pathway.
Survival among patients with relapsed/refractory diffuse large B cell lymphoma treated with single-agent selinexor in the SADAL study
Authors: Maerevoet Marie , Zijlstra Josee M. , Follows George , Casasnovas Rene-Olivier , Vermaat J. S. P. , Kalakonda Nagesh , Goy Andre , Choquet Sylvain , Van Den Neste Eric , Hill Brian , Thieblemont Catherine , Cavallo Federica , De la Cruz Fatima , Kuruvilla John , Hamad Nada , Jaeger Ulrich , Caimi Paolo , Gurion Ronit , Warzocha Krzysztof , Bakhshi Sameer , Sancho Juan-Manuel , Schuster Michael , Egyed Miklos , Offner Fritz , Vassilakopoulos Theodoros P. , Samal Priyanka , Ku Matthew , Ma Xiwen , Corona Kelly , Chamoun Kamal , Shah Jatin , Shacham Sharon , Kauffman Michael G. , Canales Miguel
Published: July 2021

Patients with RR DLBCL who have received ≥ 2 lines of therapy have limited treatment options and an expected overall survival (OS) of < 6 months. The SADAL study evaluated single-agent oral selinexor in patients with RR DLBCL and demonstrated an overall response rate (ORR) of 29.1% with median duration of response (DOR) of 9.3 months. The analyses described here evaluated a number of subpopulations in order to understand how response correlates with survival outcomes in order to identify patients who could most optimally benefit from selinexor treatment. Median age was 67 years; 44.8% of patients were ≥ 70 years of age. The median OS was 9.0 months (95% CI 6.2, 13.7) at a median follow-up of 14.8 months. The median OS was not reached in patients with a CR or PR, while patients who did not respond have a median OS of 4.9 months (p < 0.0001). Patients < 70 years had an OS of 11.1 months compared with 7.8 months in patients ≥ 70 years. Among patients with or without prior ASCT, the median OS was 10.9 and 7.8 months, respectively. Among patients with disease refractory to the most recent DLBCL treatment regimen, the median OS was 7.0 months compared with 11.1 months for disease not refractory to the most recent treatment. In a patient population in which survival is expected to be < 6 months, treatment with single-agent oral selinexor was associated with a median survival of 9 months. Increased median OS observed in patients responding to selinexor was consistent across subgroups regardless of age, prior ASCT therapy, or refractory status. Randomized studies of selinexor in combination with a variety of other anti-DLBCL agents are planned. This trial was registered at ClinicalTrials.gov (NCT02227251) on August 28, 2014.https://clinicaltrials.gov/ct2/show/NCT02227251.
Children should be seen and also heard: an explorative qualitative study into the influences on children's choice of footwear, their perception of comfort and the language they use to describe footwear experiences
Authors: Price Carina , Skidmore Sue , Ratcliffe Jane , Williams Anita
Published: July 2021

Background

Footwear has an essential role including protection of the feet, overall performance, foot health and potentially, supporting normal development of the foot. In addition to these physical aspects which may influence choice of footwear design, there are psychological influences on what a person chooses to wear. The concept of footwear ‘comfort’ spans physical and psychological perceptions of comfort in adults. However, there is little understanding of what influences children's footwear choices, how children perceive footwear comfort, or the language used to describe footwear experiences. Therefore, this study aimed to explore these three parameters as the first step to informing the development of a scale to measure footwear comfort in children.

Methods

A pragmatic qualitative design with thematic analysis as an analytical approach was implemented. Passive observation and short interviews were carried out with 23 children (aged 1–12 years) at a footwear manufactures headquarters and store. Prompts included shoes being tried on and field‐notes were taken relating to verbal and non‐verbal communication. Field notes were coded then themes were identified, reviewed and named.

Results

Overall, the children equated comfort to softness. However, influences on footwear choice were multidimensional including aesthetics, psychosocial influences, identified ‘comfort’ and ‘discomfort’ areas, practical issues and predictive concerns; all interacting with the age of the child.

Conclusions

For children, footwear comfort is a complex phenomenon having physical, cognitive, social and emotional developmental components. This can be seen in how the children perceive the ‘feel’ of the shoe and how the shoe is assessed in the context of how the shoe meets the child's physical and psychosocial developmental needs. In younger children footwear preference is related to idiosyncratic tastes in aesthetics, physical ability and comfort. As children age, societal influences begin to expand the social function of footwear denoting group membership, to include themes that transcend the functional and social function of footwear. The knowledge from this study can inform the development of age group specific tools to evaluate comfort.
Cryogenic preservation of Dirofilaria immitis microfilariae, reactivation and completion of the life-cycle in the mosquito and vertebrate hosts
Authors: Zinser Erich W. , McTier Tom L. , Kernell Nicole S. , Woods Debra J.
Published: July 2021

Background

The cryopreservation of filarial nematodes has been studied for nearly 70 years. Largely, these studies examined the effectiveness of cryopreservation methods by using the post-thaw survival of microfilariae (mf) and the development to third-stage larvae (L3s) following inoculation into a competent insect vector. Only one study reported complete reestablishment of a filarial nematode (Brugia malayi) life-cycle in a competent vertebrate host from cryopreserved stock. Expanding on this previous research, a cryopreservation method was developed to cryopreserve the mf of the dog heartworm, Dirofilaria immitis.

Methods

A combination of cryoprotectants, dimethyl sulfoxide (DMSO) and polyvinyl pyrolidone (PVP) at 6% and 4 mM, respectively, provided acceptable post-thaw survival of mf that developed into L3s in Aedes aegypti. L3s developed from cryopreserved and freshly collected mf in mosquitoes were inoculated into ferrets and dogs and were assessed after a sufficient duration post-inoculation for development into adult heartworms.

Results

Fewer adult heartworms derived from cryopreserved stocks of mf were recovered from ferrets compared to adult heartworms derived from freshly collected mf, and the former were smaller by weight and length. The onset of patency (circulating mf) occurred at similar post-inoculation time points and at similar mf densities in dogs infected with L3s sourced from cryopreserved stocks or freshly collected mf. Adults derived from cryopreserved mf have survived and produced viable mf for more than 3 years in dogs. Approximately 60% of inoculated L3s were recovered as adults from dogs at 2 and 3.5 years post-inoculation.

Conclusions

The results from these direct comparisons demonstrate that cryopreserved mf can develop into L3s in vector mosquitoes and that these L3s are infective to both dogs and ferrets, where they undergo normal development into adult worms. These worms are able to mate and produce viable mf and complete the heartworm lifecycle in dog.

Graphical Abstract
Ethmoid glomangioma and oncogenic osteomalacia: a case report
Authors: Muniz Camila R. , Bezerra Gabriela A. M. , da Silva Viviane C. , Aguiar Priscilla M. F. , Gerson Gunter , D’Alva Catarina B. , Nunes André A. A.
Published: July 2021

Background

Glomangioma is a benign tumor of mesenchymal origin, derived from the glomus body. It is responsible for the thermal regulation of the dermis. The occurrence of oncogenic osteomalacia related to glomangioma is rare. Only two cases have been reported thus far.

Case presentation

A 32-year-old female, Brazilian, presented diffuse pain, during pregnancy, that developed progressively, limiting her mobility. Imaging showed a femoral neck fracture, and rheumatological laboratory examination showed hypophosphatemia. Also, the patient reported episodes of epistaxis during childhood and recurrence along with progressively right nasal obstruction. Endoscopic resection of the tumor was performed, and immunohistochemistry was conclusive for glomangioma. This case report describes the third case in which endonasal endoscopic surgery resulted in a favorable outcome.

Conclusion

This case of glomangioma-induced oncogenic osteomalacia suggests that surgeons and clinicians should consider sinonasal tumors as a differential diagnosis of osteomalacia, and endonasal endoscopic surgery should be a possible curative resection.
Cell therapy in patients with COVID-19 using Wharton’s jelly mesenchymal stem cells: a phase 1 clinical trial
Authors: Saleh Mahshid , Vaezi Amir Abbas , Aliannejad Rasoul , Sohrabpour Amir Ali , Kiaei Seyedeh Zahra Fotook , Shadnoush Mahdi , Siavashi Vahid , Aghaghazvini Leila , Khoundabi Batoul , Abdoli Shahriyar , Chahardouli Bahram , Seyhoun Iman , Alijani Neda , Verdi Javad
Published: July 2021

Background

Mesenchymal stem cells (MSCs) have received particular attention because of their ability to modulate the immune system and inhibit inflammation caused by cytokine storms due to SARS-CoV-2. New alternative therapies may reduce mortality rates in patients with COVID19. This study aimed to assess the safety and efficacy of injecting intravenous Wharton’s jelly-derived MSCs in patients with COVID-19 as a treatment.

Methods

In this study, five patients with severe COVID-19 were treated with Wharton’s jelly-derived mesenchymal stem cells (150 × 106 cells per injection). These patients were subject to three intravenous injections 3 days apart, and monitoring was done on days 0, 3, 6, and 14 in routine tests, inflammatory cytokines, and flow cytometry of CD4 and CD8 markers. A lung CT scan was performed on base and days 14 and 28. In addition, IgM and IgG antibodies against SARS-CoV-2 were measured before and after treatment.

Results

The results showed that IL-10 and SDF-1 increased after cell therapy, but VEGF, TGF-β, IFN-γ, IL-6, and TNFα decreased. Routine hematology tests, myocardial enzyme tests, biochemical tests, and inflammation tests were performed for all patients before and after cell therapy on base and days 3, 6, and 14, which indicated the improvement of test results over time. COVID-19 antibody tests rose in 14 days after WJ-MSC injection. The total score of zonal involvement in both lungs was improved.

Conclusions

In patients, the trend of tests was generally improving, and we experienced a reduction in inflammation. No serious complications were observed in patients except the headache in one of them, which was resolved without medication. In this study, we found that patients with severe COVID-19 in the inflammatory phase respond better to cell therapy. More extensive clinical trials should be performed in this regard.

Trial registration

IRCT, IRCT20190717044241N2. Registered April 22, 2020.
Optimal measurement budget allocation for Kalman prediction over a finite time horizon by genetic algorithms
Authors: Aspeel Antoine , Legay Axel , Jungers Raphaël M. , Macq Benoit
Published: July 2021

In this paper, we address the problem of optimal measurement budget allocation to estimate the state of a linear discrete-time dynamical system over a finite horizon. More precisely, our aim is to select the measurement times in order to minimize the variance of the estimation error over a finite horizon. In addition, we investigate the closely related problem of finding a trade-off between number of measurements and signal to noise ratio.First, the optimal measurement budget allocation problem is reduced to a deterministic combinatorial program. Then, we propose a genetic algorithm implementing a count preserving crossover to solve it. On the theoretical side, we provide a one-dimensional analysis that indicates that the benefit of using irregular measurements grows when the system is unstable or when the process noise becomes important. Then, using the duality between estimation and control, we show that the problem of selecting optimal control times for a linear quadratic regulator can be reduced to our initial problem.Finally, numerical implementations demonstrate that using measurement times optimized by our genetic algorithm gives better estimate than regularly spaced measurements. Our method is applied to a discrete version of a continuous-time system and the impact of the discretization time step is studied. It reveals good convergence properties, showing that our method is well suited to both continuous-time and discrete-time setups.
Secure and efficient transmission of data based on Caesar Cipher Algorithm for Sybil attack in IoT
Authors: Thuluva Aditya Sai Srinivas , Somanathan Manivannan Sorakaya , Somula Ramasubbareddy , Sennan Sankar , Burgos Daniel
Published: July 2021

The Internet of Things (IoT) is an emerging concept in the field of information technology. IoT can integrate any real-time entity with another, using sensing, computing and communication capabilities to offer enhanced services in everyday life. In this article, IoT-based patient health monitoring is considered for use in IoT sensors deployed in devices. These devices are attached to the body of the patient for timely tracking of his or her health condition. During data transfers from devices connected to the patient’s body to the doctor, the data may be susceptible to security threats. IoT devices are subjected to many routing attacks, like blackhole, greyhole, Sybil, sinkhole and wormhole attacks. Sybil attacks are the most dangerous routing attacks. This type of attack involves stealing the identities of legitimate nodes; this, in turn, leads to information loss, misinterpretation in the network and an increase in routing disturbances. Hence, in this paper, we propose the use of the traditional Caesar Cipher Algorithm (CCA) along with the lightweight encryption algorithm (LEA) and the Received Signal Strength Indicator (RSSI) to detect and prevent Sybil attacks in an IoT environment. The proposed algorithm detects the false node in a particular path by announcing the attack to another node. It also prevents the attack by choosing an alternative path by which to forward data packets to the desired users. To ensure authentication, privacy and data integrity, the lightweight encryption algorithm with a 64-bit key is used with AODV as the routing protocol.
An SEIR model with infected immigrants and recovered emigrants
Authors: Witbooi Peter J.
Published: July 2021

We present a deterministic SEIR model of the said form. The population in point can be considered as consisting of a local population together with a migrant subpopulation. The migrants come into the local population for a short stay. In particular, the model allows for a constant inflow of individuals into different classes and constant outflow of individuals from the R-class. The system of ordinary differential equations has positive solutions and the infected classes remain above specified threshold levels. The equilibrium points are shown to be asymptotically stable. The utility of the model is demonstrated by way of an application to measles.
Language training for oral and written naming impairment in primary progressive aphasia: a review
Authors: Pagnoni Ilaria , Gobbi Elena , Premi Enrico , Borroni Barbara , Binetti Giuliano , Cotelli Maria , Manenti Rosa
Published: July 2021

Background

Primary progressive aphasia (PPA) is a neurodegenerative disorder characterized by a gradual, insidious and progressive loss of language abilities, with naming difficulties being an early and persistent impairment common to all three variants. In the absence of effective pharmacological treatments and given the progressive nature of the disorder, in the past few decades, many studies have investigated the effectiveness of language training to minimize the functional impact of word-finding difficulties in daily life.

Main body

We review language treatments most commonly used in clinical practice among patients with different variants of PPA, with a focus on the enhancement of spoken and written naming abilities. Generalization of gains to the ability to name untrained stimuli or to other language abilities and the maintenance of these results over time are also discussed. Forty-eight studies were included in this literature review, identifying four main types of language treatment: a) lexical retrieval treatment, b) phonological and/or orthographic treatment, c) semantic treatment, and d) a multimodality approach treatment. Overall, language training is able to induce immediate improvements of naming abilities in all variants of PPA. Moreover, despite the large variability among results, generalization and long-term effects can be recorded after the training. The reviewed studies also suggest that one factor that determines the choice of a particular approach is the compromised components of the lexical/semantic processing system.

Conclusion

The majority of studies have demonstrated improvements of naming abilities following language treatments. Given the progressive nature of PPA, it is essential to apply language treatment in the early stages of the disease.
Toward a behavioral approach of international shipping: a study of the inter-organisational dynamics of maritime safety
Authors: Fulconis François , Lissillour Raphael
Published: July 2021

Classification societies play a major role in maritime safety and the regulation of the international shipping market. They have a dual mission, namely the classification and certification of ships. Paradoxically, the academic literature on the strategic behaviour of classification societies remains very limited. More often than not, the scope of prior research has been limited to the definition of their missions in the shipping ecosystem with an emphasis on their changing legitimacy as maritime accidents occur. Consequently, this paper aims at providing a better understanding of the specific role of classification societies in maritime safety and within the inter-organisational dynamics of international shipping. The study is based on a conceptual framework provided by the behaviourist approach and applied to the inter-organisational dynamics of supply chains. This approach enables in-depth analysis of actors’ strategic behaviours by focusing on four dimensions: power, leadership, conflict and cooperation. The main results highlight the increasingly central and paradoxical role of classification societies. This role encompasses, on the national level, classification and certification processes, and, on the supranational level, the creation of new rules and regulations. The study highlights the importance of their ability to master the official framework and institutional vocabulary, which enable them to strengthen their power and leadership in the shipping market. This capacity helps them to limit conflicts between actors and to encourage certain cooperative behaviours based on relationships of dependence and inter-organisational interdependence.
CLeaR: An adaptive continual learning framework for regression tasks
Authors: He Yujiang , Sick Bernhard
Published: July 2021

Catastrophic forgetting means that a trained neural network model gradually forgets the previously learned tasks when being retrained on new tasks. Overcoming the forgetting problem is a major problem in machine learning. Numerous continual learning algorithms are very successful in incremental learning of classification tasks, where new samples with their labels appear frequently. However, there is currently no research that addresses the catastrophic forgetting problem in regression tasks as far as we know. This problem has emerged as one of the primary constraints in some applications, such as renewable energy forecasts. This article clarifies problem-related definitions and proposes a new methodological framework that can forecast targets and update itself by means of continual learning. The framework consists of forecasting neural networks and buffers, which store newly collected data from a non-stationary data stream in an application. The changed probability distribution of the data stream, which the framework has identified, will be learned sequentially. The framework is called CLeaR (ContinualLearning forRegression Tasks), where components can be flexibly customized for a specific application scenario. We design two sets of experiments to evaluate the CLeaR framework concerning fitting error (training), prediction error (test), and forgetting ratio. The first one is based on an artificial time series to explore how hyperparameters affect the CLeaR framework. The second one is designed with data collected from European wind farms to evaluate the CLeaR framework’s performance in a real-world application. The experimental results demonstrate that the CLeaR framework can continually acquire knowledge in the data stream and improve the prediction accuracy. The article concludes with further research issues arising from requirements to extend the framework.
Identification of theTP53p.R337H Variant in Tumor Genomic Profiling Should Prompt Consideration of Germline Testing for Li-Fraumeni Syndrome
Authors: Sandoval Renata Lazari , Masotti Cibele , de Macedo Mariana Petaccia , Ribeiro Maurício Fernando Silva Almeida , Leite Ana Carolina Rathsam , Meireles Sibele Inacio , Bovolin Rodrigo Medeiros , Santini Fernando Costa , Munhoz Rodrigo Ramella , Jardim Denis Leonardo Fontes , Katz Artur , Camargo Anamaria Aranha , Fernandes Gustavo dos Santos , Achatz Maria Isabel
Published: July 2021

PURPOSE

Li-Fraumeni syndrome (LFS) is rare in the worldwide population, but it is highly prevalent in the Brazilian population because of a founder mutation, TP53 p.R337H, accounting for 0.3% of south and southeastern population. Clinical criteria for LFS may not identify all individuals at risk of carrying the Brazilian founder mutation because of its lower penetrance and variable expressivity. This variant is rarely described in databases of somatic mutations. Somatic findings in tumor molecular profiling may give insight to identify individuals who might be carriers of LFS and allow the adoption of risk reduction strategies for cancer.

MATERIALS AND METHODS

We determined the frequency of the TP53 p.R337H variant in tumor genomic profiling from 755 consecutive Brazilian patients with pan-cancer. This is a retrospective cohort from January 2013 to March 2020 at a tertiary care center in Brazil.

RESULTS

The TP53 p.R337H variant was found in 2% (15 of 755) of the samples. The mutation allele frequency ranged from 30% to 91.7%. A total of seven patients were referred for genetic counseling and germline testing after tumor genomic profiling results were disclosed. All the patients who proceeded with germline testing (6 of 6) confirmed the diagnosis of LFS. Family history was available in 12 cases. Nine patients (9 of 12) did not meet LFS clinical criteria.

CONCLUSION

The identification of the TP53 p.R337H variant in tumor genomic profiling should be a predictive finding of LFS in the Brazilian population and should prompt testing for germline status confirmation.
Endocrine Disrupting Chemicals and Risk of Testicular Cancer: A Systematic Review and Meta-analysis
Authors: Bräuner Elvira V , Lim Youn-Hee , Koch Trine , Uldbjerg Cecilie S , Gregersen Laura S , Pedersen Marc K , Frederiksen Hanne , Petersen Jørgen H , Coull Brent A , Andersson Anna-Maria , Hickey Martha , Skakkebæk Niels E , Hauser Russ , Juul Anders
Published: July 2021

The incidence of many hormone-dependent diseases, including testicular cancer, has sharply increased in all high-income countries during the 20th century. This is not fully explained by established risk factors. Concurrent, increasing exposure to antiandrogenic environmental endocrine disrupting chemicals (EDCs) in fetal life may partially explain this trend. This systematic review assessed available evidence regarding the association between environmental EDC exposure and risk of testicular cancer (seminomas and nonseminomas). Following PRISMA guidelines, a search of English peer-reviewed literature published prior to December 14, 2020 in the databases PubMed and Embase® was performed. Among the 279 identified records, 19 were eligible for quality assessment and 10 for further meta-analysis. The completeness of reporting was high across papers, but over 50% were considered subject to potential risk of bias. Mean age at diagnosis was 31.9 years. None considered effects of EDC multipollutant mixtures. The meta-analyses showed that maternal exposure to combined EDCs was associated with a higher risk of testicular cancer in male offspring [summary risk ratios: 2.16, (95% CI:1.78-2.62), 1.93 (95% CI:1.49-2.48), and 2.78 (95% CI:2.27-3.41) for all, seminoma, and nonseminoma, respectively]. Similarly, high maternal exposures to grouped organochlorines and organohalogens were associated with higher risk of seminoma and nonseminoma in the offspring. Summary estimates related to postnatal adult male EDC exposures were inconsistent. Maternal, but not postnatal adult male, EDC exposures were consistently associated with a higher risk of testicular cancer, particularly risk of nonseminomas. However, the quality of studies was mixed, and considering the fields complexity, more prospective studies of prenatal EDC multipollutant mixture exposures and testicular cancer are needed.
STAU2 binds a complex RNA cargo that changes temporally with production of diverse intermediate progenitor cells during mouse corticogenesis
Authors: Chowdhury Rebecca , Wang Yue , Campbell Melissa , Goderie Susan K. , Doyle Francis , Tenenbaum Scott A. , Kusek Gretchen , Kiehl Thomas R. , Ansari Suraiya A. , Boles Nathan C. , Temple Sally
Published: July 2021

STAU2 is a double-stranded RNA-binding protein enriched in the nervous system. During asymmetric divisions in the developing mouse cortex, STAU2 preferentially distributes into the intermediate progenitor cell (IPC), delivering RNA molecules that can impact IPC behavior. Corticogenesis occurs on a precise time schedule, raising the hypothesis that the cargo STAU2 delivers into IPCs changes over time. To test this, we combine RNA-immunoprecipitation with sequencing (RIP-seq) over four stages of mouse cortical development, generating a comprehensive cargo profile for STAU2. A subset of the cargo was ‘stable’, present at all stages, and involved in chromosome organization, macromolecule localization, translation and DNA repair. Another subset was ‘dynamic’, changing with cortical stage, and involved in neurogenesis, cell projection organization, neurite outgrowth, and included cortical layer markers. Notably, the dynamic STAU2 cargo included determinants of IPC versus neuronal fates and genes contributing to abnormal corticogenesis. Knockdown of one STAU2 target, Taf13, previously linked to microcephaly and impaired myelination, reduced oligodendrogenesis in vitro. We conclude that STAU2 contributes to the timing of corticogenesis by binding and delivering complex and temporally regulated RNA cargo into IPCs.
The total mRNA concentration buffering system in yeast is global rather than gene-specific
Authors: García-Martínez José , Medina Daniel A. , Bellvís Pablo , Sun Mai , Cramer Patrick , Chávez Sebastián , Pérez-Ortín José E.
Published: July 2021

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one.
A large-scale image dataset of wood surface defects for automated vision-based quality control processes
Authors: Kodytek Pavel , Bodzas Alexandra , Bilik Petr
Published: July 2021

The wood industry is facing many challenges. The high variability of raw material and the complexity of manufacturing processes results in a wide range of visible structure defects, which have to be controlled by trained specialists. These manual processes are not only tedious and biased, but also less effective. To overcome the drawbacks of the manual quality control processes, several automated vision-based systems have been proposed. Even though some conducted studies achieved a higher recognition rate than trained experts, researchers have to deal with a lack of large-scale databases and authentic data in this field. To address this issue, we performed a data acquisition experiment set in the industrial environment, where we were able to acquire an extensive set of authentic data from a production line. For this purpose, we designed and implemented a complex technical solution suitable for high-speed acquisition during harsh manufacturing conditions. In this data note, we present a large-scale dataset of high-resolution sawn timber surface images containing more than 43 000 labelled surface defects and covering 10 types of the most common wood defects. Moreover, with each image record, we provide two types of labels allowing researchers to perform semantic segmentation, as well as defect classification, and localization.