bluuebunny/crossref_metadata_2025 huggingface dataset:
Career Exploration and Career Decision Self-Efficacy in Northwest Chinese Pre-service Kindergarten Teachers: The Mediating Role of Work Volition and Career Adaptability
Authors: Zhao Fangfang , Li Ping , Chen Siyuan , Hao Yijun , Qin Jinliang
Published: January 2022

Studies have documented that career exploration is significantly associated with CDSE, but how this association occurred is not clear yet. This study committed to clarifying the mechanism underlying the relationship between career exploration and CDSE by investigating the mediation effect of work volition and career adaptability among 586 pre-service kindergarten teachers. The participants are recruited from Ningxia Hui Autonomous Region in northwest China, covering Han, Hui, and other minorities. They took part in a two-wave (6 months apart) longitudinal survey and reported on their career exploration at T1, work volition, career adaptability, and career decision self-efficacy (CDSE) at T2. Results showed that T1 career exploration is directly related to the T2 CDSE. Further, career exploration contributed to the CDSE through both the separated mediation path and the chained mediation path of T2 work volition and T2 career adaptability. The results suggest that individuals who engage in more career exploration activities are likely to have more confidence in their abilities to make career decisions over time, which was partially and serially explained by individuals’ perception of capacity despite constraints and greater self-regulatory strength. This study is a first attempt to deeply clarify the link between career exploration and CDSE, and the findings shed light on the independent and serial mediating effects of work volition and career adaptability. The implications and limitations are discussed.
Wuhan College Students’ Self-Directed Learning and Academic Performance: Chain-Mediating Roles of Optimism and Mental Health
Authors: Li Jun , Yang Dong , Hu Ziao
Published: January 2022

This study explored the chain-mediating roles of optimism and mental health in the relation of self-directed learning with academic performance among college students in Wuhan during long-term online teaching. In total, 473 valid responses were obtained from students at three Wuhan universities. Self-directed learning, optimism, mental health, and academic performance scales were used as measurement instruments; a 5-point Likert scale was employed for all items. To examine the instruments’ reliability and validity, a measurement model was constructed; moreover, structural models were employed for assessing the chain mediation model. This study confirmed that self-directed learning was a positive predictor of academic performance in Wuhan college students. Optimism and mental health were two mediators and partially jointly mediated the relation of self-directed learning with academic performance. The results revealed that self-directed learning only partially positively predicted academic performance. The aforementioned relationship was partially mediated by optimism and mental health, highlighting the essential roles of optimism and mental health in the learning and learning outcomes of Wuhan college students.
Resistance to Change as a Mediator Between Conscientiousness and Teachers’ Job Satisfaction. The Moderating Role of Learning Goals Orientation
Authors: Paloş Ramona , Vîrgă Delia , Craşovan Mariana
Published: January 2022

Teachers’ job satisfaction has been the subject of many studies that tried to identify its main sources. Based on the social cognitive career theory, the present study aimed to investigate the relationships between personality traits, goals orientation, and teachers’ job satisfaction. A total of 321 Romanian teachers completed an online questionnaire. The results demonstrated new insights regarding the relationships between psychological variables (conscientiousness, dispositional resistance to change, and achievement goals orientation) and teachers’ job satisfaction. Cognitive rigidity, as a mechanism to resistance to change, mediates between conscientiousness and teachers’ job satisfaction. Moreover, the moderation role of learning goals orientation manifests in the relation between conscientiousness and job satisfaction. These findings emphasize that school management needs to offer teachers information and explain the change’s benefits if they want to prevent individual resistance to change and decrease satisfaction related to their work.
Relationship Dynamics of Couples Facing Advanced-Stage Parkinson’s Disease: A Dyadic Interpretative Phenomenological Analysis
Authors: Constant Emilie , Brugallé Elodie , Wawrziczny Emilie , Sokolowski Céline , Manceau Charlotte , Flinois Bérengère , Baille Guillaume , Luc Defebvre , Dujardin Kathy , Antoine Pascal
Published: January 2022

Background/Objective

Several studies have examined the impact of Parkinson’s disease (PD) on the quality of couples’ relationships. To date, few studies have explored how couples experience their relationship dynamic by taking into account the disease stage. The objectives of this study were to understand the experience of each partner and to study the mechanisms that underlie their couple organization in the advanced stage of PD.

Methods

Semistructured individual interviews conducted with fifteen patients and their partners were the subject of a dyadic interpretative phenomenological analysis.

Results

Three themes were identified from the analysis: the first, “A Closeness That Separates,” allows the identification of different patterns of interactions that lead to emotional distancing between the partners; the second, “The Adversity Is Not Unbearable, But Going It Alone Would Be,” emphasizes the importance of how the assisting partner provides support; and the third, “Be Prepared for Anything and Facing an Uncertain Future,” reveals the extent and modes of the dyadic regulation of the emotions linked to what the future may hold.

Conclusion

Psychological support is important to help couples express both their feelings and their respective needs in the evolving context of PD.
Bio-Psychological Predictors of Acute and Protracted Fatigue After Burns: A Longitudinal Study
Authors: Boersma-van Dam Elise , Engelhard Iris M. , van de Schoot Rens , Van Loey Nancy E. E.
Published: January 2022

Objective

Fatigue after burns is often attributed to the hyperinflammatory and hypermetabolic response, while it may be best understood from a bio-psychological perspective, also involving the neuro-endocrine system. This longitudinal multi-center study examined the course of fatigue up to 18 months postburn. The contribution of bio-psychological factors, including burn severity, pain, and acute PTSD symptoms, to the course and persistence of fatigue was studied in a multifactorial model.

Methods

Participants were 247 adult burn survivors. Fatigue symptoms were assessed with the Multidimensional Fatigue Inventory during the acute phase and subsequently at 3, 6, 12, and 18 months postburn, and were compared to population norms. Age, gender, burn severity, acute PTSD symptoms and pain were assessed as potential predictors of fatigue over time in a latent growth model.

Results

At 18 months postburn, 46% of the burn survivors reported fatigue, including 18% with severe fatigue. In the acute phase, higher levels of fatigue were related to multiple surgeries, presence of pain, and higher levels of acute PTSD symptoms. Fatigue gradually decreased over time with minor individual differences in rate of decrease. At 18 months, pain and acute PTSD symptoms remained significant predictors of fatigue levels.

Conclusions

Protracted fatigue after burns was found in almost one out of five burn survivors and was associated with both pain and acute PTSD symptoms. Early detection of PTSD symptoms and early psychological interventions aimed at reducing PTSD symptoms and pain may be warranted to reduce later fatigue symptoms.
Healthy Middle-Aged Adults Have Preserved Mnemonic Discrimination and Integration, While Showing No Detectable Memory Benefits
Authors: Samrani George , Lundquist Anders , Pudas Sara
Published: January 2022

Declarative memory abilities change across adulthood. Semantic memory and autobiographic episodic knowledge can remain stable or even increase from mid- to late adulthood, while episodic memory abilities decline in later adulthood. Although it is well known that prior knowledge influences new learning, it is unclear whether the experiential growth of knowledge and memory traces across the lifespan may drive favorable adaptations in some basic memory processes. We hypothesized that an increased reliance on memory integration may be an adaptive mechanism to handle increased interference from accumulating memory traces and knowledge across adulthood. In turn, this may confer an improved ability for integration, observable in middle-age, before the onset of major aging-related declines. We further tested whether the hypothesized increase would be associated with previously observed reductions in memory discrimination performance in midlife. Data from a sample of healthy middle-aged (40–50 years, n = 40) and younger adults (20–28 years, n = 41) did not support the hypothesis of improved integration, as assessed by an associative inference paradigm. Instead, age-equivalent performance on both integration and discrimination measures were observed [Bayes factors (BFs)10 = 0.19–0.25], along with expected higher verbal knowledge and slower perceptual speed for middle-aged [(BFs)10 = 8.52–73.52]. The results contribute to an increased understanding of memory processing in midlife, an understudied portion of the lifespan, and suggest that two core episodic memory processes, integration and discrimination, can be maintained in healthy middle-aged adults.
Time-Based Binding as a Solution to and a Limitation for Flexible Cognition
Authors: Senoussi Mehdi , Verbeke Pieter , Verguts Tom
Published: January 2022

Why can’t we keep as many items as we want in working memory? It has long been debated whether this resource limitation is a bug (a downside of our fallible biological system) or instead a feature (an optimal response to a computational problem). We propose that the resource limitation is a consequence of a useful feature. Specifically, we propose that flexible cognition requires time-based binding, and time-based binding necessarily limits the number of (bound) memoranda that can be stored simultaneously. Time-based binding is most naturally instantiated via neural oscillations, for which there exists ample experimental evidence. We report simulations that illustrate this theory and that relate it to empirical data. We also compare the theory to several other (feature and bug) resource theories.
Factors Related to the Length of Stay for Patients With Schizophrenia: A Retrospective Study
Authors: Cheng Peng , Wang Lirong , Xu Lizhi , Zhou Ying , Zhang Li , Li Weihui
Published: January 2022

Background

The length of stay (LOS) of patients with schizophrenia has been a wide concern of researchers. Reasonable management of the LOS to achieve a balance between quality of treatment and efficient medical source allocation has become a significant issue in clinical work in psychiatry. Figuring out the factors related to the LOS of schizophrenia patients can help optimize its management by the hospital.

Method

The essential information of patients was obtained from the electronic medical record system. The variables were divided into the following kinds: demographic, clinical, and biochemical. Univariate analysis and multivariate analysis were conducted to find the potential factors related to the LOS of schizophrenia patients. Receiver operating characteristic analyses were conducted to evaluate the accuracy of judging the LOS of the regression model.

Result

A total of 1,160 patients with schizophrenia were enrolled in our research. Our results demonstrated that the status of unmarried (single, separated, divorced, or widowed) and the abnormality of thyroid-stimulating hormone (TSH) were risk factors for the longer LOS of schizophrenia patients. The area under the curve was 0.576, which meant that the regression model had a certain predictive value.

Conclusion

To our knowledge, this research is the first study to analyze the effect of various factors, including the biochemical index, on the LOS of a single type of mental disorder. Marital status and TSH were proven to be related to the LOS of schizophrenia patients. The results of this study provided reference factors of LOS for clinical psychiatry, which will be helpful to the management of hospitalization and in optimizing the allocation of medical sources.
Japan's Slow Response to Improve Access to Inpatient Care for COVID-19 Patients
Authors: Nakahara Shinji , Inada Haruhiko , Ichikawa Masao , Tomio Jun
Published: January 2022

The coronavirus disease 2019 (COVID-19) pandemic has exposed various weaknesses in national healthcare systems across the globe. In Japan, this includes the inability to promptly mobilize the resources needed to provide inpatient care in response to the rapidly increasing number of patients. Combined with unclear entry points to healthcare, particularly in emergency cases, this has led to a situation in which access to healthcare is rapidly deteriorating. This study examined problems in Japan's healthcare delivery system. While Japan's healthcare resources (e.g., hospital beds and medical personnel) are comparable to those found in other high-income countries, progress has been slow in securing beds for COVID-19 patients. In addition, the number of beds has only recently reached the levels seen in Western countries. Factors related to slow resource allocation include dispersed existing medical resources (mainly in the private sector), the lack of collaboration mechanisms among private-dominant healthcare providers and public health agencies, an inadequate legal framework for resource mobilization, the insufficient quantification of existing resources, and undesignated entry points to healthcare systems. To better prepare for future disasters, including the next wave of COVID-19, Japan urgently needs to restructure its legal framework to promptly mobilize resources, accurately quantify existing resources, introduce coordination mechanisms with functional differentiations among all community stakeholders, and clearly designate entry points to healthcare.
Health Literacy and Migrant Communities in Primary Health Care
Authors: Medina Patrícia , Maia Ana Catarina , Costa Andreia
Published: January 2022

Introduction

The promotion of health literacy of the population in a situation of migration, in the community, is a fundamental field of intervention in health promotion, for the reduction of inequalities in access to health care services. It is increasingly necessary to make health care services more equitable for migrant populations. The aim of the study was to characterize the level of health literacy of the population in a migrant situation, attending a primary health care unit in the Lisbon region, to identify priority areas for community intervention that will become the focus of intervention and contribute to the increase in the health literacy levels in this population.

Methods

A cross-sectional study was carried out by applying the Health Literacy Survey (ILS-PT) to a sample of the population in a situation of migration, found by 27 participants.

Results

The general health literacy index of the sample is inadequate (21.23 points). An analysis of the sub-indexes revealed that 75% of the participants had difficulties related to information about health care and 80% had difficulties in the field of health promotion.

Conclusions

Problematic and inadequate levels of health literacy was significantly frequent among migrant population. So that enhancing health literacy among migrant is essential to reduce health inequalities to achieve better health outcomes and contribute to defense of human rights of this vulnerable population.
The Dermatological Effects and Occupational Impacts of Personal Protective Equipment on a Large Sample of Healthcare Workers During the COVID-19 Pandemic
Authors: Santoro Paolo Emilio , Borrelli Ivan , Gualano Maria Rosaria , Proietti Ilaria , Skroza Nevena , Rossi Maria Francesca , Amantea Carlotta , Daniele Alessandra , Ricciardi Walter , Potenza Concetta , Moscato Umberto
Published: January 2022

Introduction

Working during the Sars-CoV-2 pandemic healthcare workers (HCWs) had to wear Personal Protective Equipment (PPEs) for extended periods of time, leading to an increase in dermatological reactions. The study evaluates the prevalence of adverse skin reactions to PPEs among Italian healthcare workers during the COVID-19 pandemic, and aims to determine whether prolonged PPEs usage poses a significant occupational health risk, by measuring the loss of work days and the eligibility of workers that requested health surveillance due to dermatological PPEs reactions.

Materials and Methods

An online ad hoc questionnaire was administered to a sample of Italian HCWs. Questions verted on sociodemographic characteristics, PPEs usage, and occupational well-being. Descriptive analyses and logistic regressions were performed to explore possible associations between variables.

Results

Two types of PPEs, Gloves and Masks, were tested. The sample included 1,223 interviewed HCWs, 1,184 gave their consent for participation. A total of 90 medical surveillance visits were requested due to PPEs related dermatological issues: in 30 cases were recognized limitations in working duties and in one case the worker was deemed not fit to keep working. Furthermore, 25 workers had a loss of occupational days due to dermatological issues. A statistically significant correlation was observed with being a nurse or midwife (OR = 1.91, IC = 1.38–2.63, p < 0.001), and being female (OR = 2.04, IC = 1.49–2.78, p < 0.001), which acted as risk factors.

Discussion

The enhanced protection measures put in place during the COVID-19 pandemic, highlight the importance of occupational dermatology. This study could contribute to assess the issue, aiming to develop better prevention strategies in the workplace in order to improve well-being of HCWs and reduce the impact of dermatological adverse reactions to PPEs.
The Impact of Health Investment on Industrial Structure: “Spillover” or “Crowding Out”?—Evidence From Emerging Market Countries
Authors: Chen Qiao , Wei Hai-Ming , Zhi Yu-Peng
Published: January 2022

Using national dynamic panel data from 21 emerging markets between 1999 and 2020 and bidirectional fixed effect and threshold regression methods, this paper evaluated the impact of health investment on industrial structure upgrading from two aspects of economic output and economic structure. The results showed that: (1) public health investment and private health investment have a crowding out effect on the added value of primary and secondary industries, and the crowding out effect of public health investment is greater than that of private health investment; (2) Public health investment and private health investment have a spillover effect on the added value of the tertiary industry, and the spillover effect of public health investment is greater than that of private health investment; (3) Both public and private health investment contribute to the transfer of the labor force to the tertiary industry, and tests showed the baseline regression results were robust and reliable; (4) The relationship between health investment and industrial structure upgrading was non-linear. As per capital GDP increases, the inhibition effect of public health investment on industrial structure upgrading gradually weakens whilst the promotion effect of private health investment on industrial structure upgrading gradually increases. The results of this study clarify how health investment affects industrial structure, and offers new guidance for health investment policy formulation in emerging market countries.
Exploring Dissolved Organic Carbon Variations in a High Elevation Tropical Peatland Ecosystem: Cerro de la Muerte, Costa Rica
Authors: Ricardo Sánchez-Murillo , Paola Gastezzi-Arias , Rolando Sánchez-Gutiérrez , Germain Esquivel-Hernández , Roy Pérez-Salazar , María Poca
Published: January 2022

Tropical peatlands are distributed mainly in coastal lowlands; however high elevation regions exhibit a large prevalence of small and fragmented peatlands that are mostly understudied. Artificial drainage of peatlands to expand the area of cattle farming, horticulture, and urbanization is increasing carbon losses to the atmosphere and streams worldwide. Here, we present an exploratory characterization of dissolved carbon optical properties in ombrotrophic peat bogs of the Talamanca range of Costa Rica, across an altitudinal gradient (2,400–3,100 m a.s.l.) during the rainy season. Dissolved organic matter (DOM) sources and decomposition processes were evaluated in the light of dissolved organic and inorganic carbon (DOC and DIC), optical properties, and major water chemistry. DOC concentrations ranged from 0.2 up to 47.0 mg/L. DIC concentrations were below 2 mg/L and δ13CDIC values indicated a mixture between soil organic matter, CO2 in soil water, and to a lesser degree DIC derived from bacterial CO2. Absolute fluorescence intensity of humic-like peaks was 6–7 times greater than fresh-like peaks across all sites. Fluorescence peak ratios coupled with the biological and humification indexes point to a greater relative contribution of recalcitrant soil-derived DOM. Excitation/Emission matrices denoted a high prevalence of humic and fulvic acids in the peat bogs, with moderate intensities in soluble microbial by-products-like and aromatic protein regions at three sites. Our data provides a baseline to underpin tropical carbon dynamics across high elevation peatlands.
miRNA Sequence Analysis in Patients With Kaposi’s Sarcoma-Associated Herpesvirus
Authors: Tuncer Seref Bugra , Celik Betul , Akdeniz Odemis Demet , Kılıc Erciyas Seda , Sukruoglu Erdogan Ozge , Avsar Mukaddes , Kuru Turkcan Gozde , Yazici Hulya
Published: January 2022

MicroRNAs (miRNAs) are the non-coding RNAs that can both attach to the untranslated and coding sections of target mRNAs, triggering destruction or post-transcriptional alteration. miRNAs regulate various cellular processes such as immune function, apoptosis, and tumorigenesis. About 35,000 miRNAs have been discovered in the human genome. The increasing evidence suggests that the dysregulation of human miRNAs may have a role in the etiology of some disorders including cancer. Only a small sub-set of human miRNAs has functionally been validated in the pathogenesis of oncogenic viruses such as Kaposi’s sarcoma-associated herpesvirus (KSHV). KSHV is the cause of various human malignancies including primary effusion lymphoma (PEL) and Kaposi’s sarcoma (KS), which are mainly seen in AIDS patients or other immunocompromised people. We aimed to identify the miRNAs in Kaposi’s sarcoma cases, with the comparison of KSHV seropositive and seronegative tumors with the controls and in each other in Turkish Kaposi’s sarcoma patients. We performed the miRNA-sequencing at genome level in the peripheral blood mononuclear cells of 16 Kaposi’s sarcoma patients, and in 8 healthy controls matched for age, gender, and ethnicity. A total of 642 miRNA molecules with different expression profiles were identified between the patients and the healthy controls. Currently, out of 642 miRNAs, 7 miRNAs (miR-92b-3p, miR-490-3p, miR-615-3p, miR-629-5p, miR-1908, miR-3180, miR-4433b-3p) which have not been described in the literature in the context of Kaposi’s sarcoma were addressed in the study for the first time and 9 novel miRNAs, not found previously in the database, have been detected in Kaposi’s sarcoma using the miRNA-sequencing technique. This study demonstrates the identification of differently expressed miRNAs which might be the new therapeutic targets for Kaposi’s sarcoma, that has limited treatment options and can be used in the etiology, diagnosis, and prognosis of this cancer.
Design, Computational Modelling and Experimental Characterization of Bistable Hybrid Soft Actuators for a Controllable-Compliance Joint of an Exoskeleton Rehabilitation Robot
Authors: Dragone Donatella , Randazzini Luigi , Capace Alessia , Nesci Francesca , Cosentino Carlo , Amato Francesco , De Momi Elena , Colao Roberto , Masia Lorenzo , Merola Alessio
Published: January 2022

This paper presents the mechatronic design of a biorobotic joint with controllable compliance, for innovative applications of “assist-as-needed” robotic rehabilitation mediated by a wearable and soft exoskeleton. The soft actuation of robotic exoskeletons can provide some relevant advantages in terms of controllable compliance, adaptivity and intrinsic safety of the control performance of the robot during the interaction with the patient. Pneumatic Artificial Muscles (PAMs), which belong to the class of soft actuators, can be arranged in antagonistic configuration in order to exploit the variability of their mechanical compliance for the optimal adaptation of the robot performance during therapy. The coupling of an antagonistic configuration of PAMs with a regulation mechanism can achieve, under a customized control strategy, the optimal tuning of the mechanical compliance of the exoskeleton joint over full ranges of actuation pressure and joint rotation. This work presents a novel mechanism, for the optimal regulation of the compliance of the biorobotic joint, which is characterized by a soft and hybrid actuation exploiting the storage/release of the elastic energy by bistable Von Mises elastic trusses. The contribution from elastic Von Mises structure can improve both the mechanical response of the soft pneumatic bellows actuating the regulation mechanism and the intrinsic safety of the whole mechanism. A comprehensive set of design steps is presented here, including the optimization of the geometry of the pneumatic bellows, the fabrication process through 3D printing of the mechanism and some experimental tests devoted to the characterization of the hybrid soft actuation. The experimental tests replicated the main operating conditions of the regulation mechanism; the advantages arising from the bistable hybrid soft actuation were evaluated in terms of static and dynamic performance, e.g., pressure and force transition thresholds of the bistable mechanism, linearity and hysteresis of the actuator response.
Strategic Background of the Start-Up—Qualitative Analysis
Authors: Slávik Štefan , Hudáková Ivana Mišúnová , Procházková Katarína , Zagoršek Branislav
Published: January 2022

A start-up is a relatively new and attractive entrepreneurial form that is being explored in a broader national economy and industry context. However, there is little knowledge about its strategy, which is mainly represented by the business development strategy. The main goal of the research is to deepen and expand knowledge about the strategic background of start-ups, which is preparation and condition for a development strategy and later a business strategy, too. The partial goals of the research are in-depth and detailed knowledge of the content and novelty of the business idea, the quality of the internal environment of the start-up, the nature of the external environment of the start-up, and the content of the goals. The research sample contains 147 start-ups operating in Slovakia. Each start-up was researched by a member of the research team, who personally recorded the statements of the founder. The research results are based on qualitative analysis and synthesis of statements of the founders of start-ups. The main result of the research is a summary view of the strategic background of the examined start-ups, which expresses the peculiarities of the start-up business making and documents the possibilities and motives of the start-up’s strategizing. The summary of knowledge about the strategic background of the start-up is synthesized into a strategic balance sheet, which expresses the strategic perspective of the start-up, the possibilities of survival, explains the potential failure, and provides a solution to the identified imbalance. The practical use of the results consists of providing a model of the strategic balance, which is the result of field research of real and functioning start-ups. The originality and value of the research lie in the direct collection of qualitative data, immediate knowledge of business reality, and the synthesis of results into a comprehensive and at the same time detailed picture of the strategic background of the start-up.
Organizational and Structural Approaches on Administrative Simplification: The Case of Kosovo
Authors: Halili Ruzhdi , Kukovič Simona
Published: January 2022

Administrative simplification is a particular approach that is considered a key aspect to ensure the quality of regulations and legislation. The appropriate organizational and structural approach to managing, coordinating, and monitoring administrative simplification is an important factor. The institutional framework for managing and coordinating administrative simplification initiatives has been the subject of discussions in the Government of Kosovo for several years. The objective of this paper is therefore to analyze the institutional set up in the central administration, as well as gaps and challenges, and their implications for the implementation of such reforms in Kosovo. It also presents the context of the reforms implemented by the Kosovo state administration to simplify administration and reduce administrative burdens. Particular attention is paid to the importance, status of implementation, and administration of the Law on General Administrative Procedures. Several recommendations and options have been made based on the findings of this study, the authors’ first-hand experience, and best comparative international practice.
Uncertainty Analysis of Parameters in SST Turbulence Model for Shock Wave-Boundary Layer Interaction
Authors: Zhang Kailing , Li Jinping , Zeng Fanzhi , Wang Qiang , Yan Chao
Published: January 2022

Shock wave-boundary layer interactions (SWBLIs) have a tremendous influence on the performance of hypersonic vehicles. For the numerical simulation of such engineering flows, Reynolds averaged Navier-Stokes (RANS) still occupies an irreplaceable role. However, parameters of turbulence models in RANS have substantial uncertainties, which impact the reliability of simulation results. Thus, the aim of the present study is to conduct an uncertainty analysis on parameters in the shear-stress transport (SST) turbulence model for the simulation of SWBLIs. In the current work, uncertainty quantification was performed first. A surrogate model was constructed by the non-intrusive polynomial chaos (NIPC) method to propagate uncertainties from model parameters to the quantities of interests (QoIs) and quantify them. In the subsequent sensitivity analysis, the key parameters were identified for such flow by calculating the Sobol index of each parameter for various QoIs. The results indicate that uncertainties of model parameters led to non-negligible uncertainties in those QoIs, particularly in skin friction and wall heat flux. The parameters α1, σω1, β1 were identified as primary contributors through the sensitivity analysis. Moreover, the specific effects of the three parameters on the flow prediction were analyzed by changing the parameters’ values separately.
Effects of Corn Intercropping with Soybean/Peanut/Millet on the Biomass and Yield of Corn under Fertilizer Reduction
Authors: Li Likun , Zou Yan , Wang Yanhui , Chen Fajun , Xing Guangnan
Published: January 2022

Corn (Zea mays L.) is one of the key grain crops in China. In fields, the two crops of soybean (Glycine max L.) and peanut (Arachis hypogaea L.), which have nitrogen-fixing capacity (NFC), are generally used to intercrop with corn to improve plant physiology and production ability of corn even under fertilizer reduction. To explore a more scientific and reasonable way to plant corn, and simultaneously reduce the use of chemical fertilizers and pesticides, the impacts of corn intercropping with two NFC crops (including soybean and peanut) and the a non-NFC crop (i.e., millet (Setaria italica)) through five planting patterns, including three intercropping patterns (2 corn rows to 2, 3, and 4 NFC-crop rows or 2, 4, and 6 millet rows) and two sole crop patterns of corn and soybean, peanut, or millet under normal (600 kg/ha) and reduced (375 kg/ha) levels of NPK (N:P2O5:K2O = 15:15:15) fertilization levels on the activity of N-metabolism-related enzymes in corn rhizosphere soil and corn leaves, and plant biomass and yield of corn were researched in this study. The results showed that fertilizer reduction significantly decreased the plant biomass and grain yield of the sole crop corn. The intercropping type and planting pattern both had significant effects on the activities of N-metabolism-related enzyme of soil alkaline protease (S-ALPT), and glutamine oxoglutarate aminotransferase (GOGAT), glutamate synthetase (GS), and nitrate reductase (NR) in the leaves of corn plants. The intercropping type of corn with soybean through the planting pattern of 2 corn rows to 4 soybean rows significantly improved the activities of N-metabolism-related enzymes in soil and corn leaves even under the fertilizer reduction. The intercropping pattern of corn-soybean was the most beneficial to increase the total nitrogen content in soil and corn leaves. In addition, the intercropping significantly increased the soil microbial diversity under normal fertilizer. Furthermore, fertilizer reduction significantly increased soil microbial diversity of the corn sole crop. Therefore, it is concluded that for corn in intercropping systems, the best and the worst companion crop were, respectively, soybean and millet.
Impact of Conservation Agriculture on Soil Erosion in the Annual Cropland of the Apulia Region (Southern Italy) Based on the RUSLE-GIS-GEE Framework
Authors: Petito Matteo , Cantalamessa Silvia , Pagnani Giancarlo , Degiorgio Francesco , Parisse Barbara , Pisante Michele
Published: January 2022

The processes of soil erosion and land degradation are more rapid in the case of inappropriate agricultural management, which leads to increased soil loss rates. Moreover, climatic conditions are one of the most important determining factors affecting agriculture, especially in the Mediterranean areas featuring irregular rainfall and high summer temperatures. Conservation agriculture (CA) can make a significant contribution to reducing soil erosion risk on the annual cropland (ACL) of the Mediterranean region in comparison with conventional management (CM). The objective of this study is to provide soil loss rate maps and calculate the values for each altitude and slope class and their combination for the Apulia region in four annual production cycles for the scenarios CM and CA. The present study estimates the significance of the adoption of CA on soil erosion assessment at regional scale based on the Revised Universal Soil Loss Equation (RUSLE) model. The parameters of the RUSLE model were estimated by using remote sensing (RS) data. The erosion probability zones were determined through a Geographic Information System (GIS) and Google Earth Engine (GEE) approach. Digital terrain model (DTM) at 8 m, ACL maps of the Apulia region, and rainfall and soil data were used as an input to identify the most erosion-prone areas. Our results show a 7.5% average decrease of soil loss rate during the first period of adoption of the four-year crop cycle—from 2.3 t ha−1 y−1 with CM to 2.1 t ha−1 y−1 with the CA system. CA reduced soil loss rate compared to CM in all classes, from 10.1% in hill class to 14.1% for hill + low slope class. These results can therefore assist in the implementation of effective soil management systems and conservation practices to reduce soil erosion risk in the Apulia region and in the Mediterranean basin more generally.
Assessing Factors Controlling Structural Changes of Humic Acids in Soils Amended with Organic Materials to Improve Soil Functionality
Authors: Amoah-Antwi Collins , Kwiatkowska-Malina Jolanta , Szara Ewa , Fenton Owen , Thornton Steven F. , Malina Grzegorz
Published: January 2022

Humic acids (HAs) regulate soil chemical reactivity and improve many soil functions. The amendment of soil with organic materials increases soil organic matter (SOM) content and promotes the formation of HAs. However, the effect of the type, frequency and duration of amendment, and pedoclimatic conditions on SOM transformation and HA structural changes remains unclear. Herein, four experimental field sites (S1–4) with short-to-long-term organic fertilisation schemes were used to assess the effects of such factors, i.e., S1: loamy sand amended once with farmyard manure (FYM), brown coal waste (BCW), and biochar (BIO) for 0.5 and 1.5 years; S2: silt loam amended once with BIO for 8 years; S3: loamy sand amended every 5 years with FYM for 94 years; and S4: clayey silt amended every 2 years with FYM for 116 years. All HAs were extracted and analysed for structural differences by elemental analysis (EA), attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR), solid-state cross polarisation magic angle spinning nuclear magnetic resonance spectroscopy (CP/MAS 13C-NMR), and differential scanning calorimetry (DSC). Results from EA, FTIR, and NMR showed that the long-term samples from S3 (treatments, T9–T10) and S4 (T11–T12) had the greatest aromatic characteristics, which increased with FYM amendment (T10 and T12). These agreed with DSC data, which indicated lower aliphatic contents compared with other samples. Samples from S2 (T7–T8), with receded amendment effects, had less aromatic and greater aliphatic characteristics compared with the short-term samples, S1 (T1–T6). In S1, structural changes were limited, but aromaticity increased with BIO (T3 and T6) compared with corresponding FYM (T1 and T4) and BCW (T2 and T5) amendments due to inherently high aromatic groups in the former. Overall, the results showed that the site (due to differences in pedoclimatic conditions), field age of OM, and amendment frequency were the main factors that influenced HA structure, and hence SOM transformation. Regular, long-term organic amendment increases the aromatic characteristics of HAs, which can improve soil functionality, but short-term structural improvements are achievable only when amending material is rich in aromatic compounds.
Mineral Fertilization and Maize Cultivation as Factors Which Determine the Content of Trace Elements in Soil
Authors: Brodowska Marzena S. , Wyszkowski Mirosław , Bujanowicz-Haraś Barbara
Published: January 2022

This study has been carried out in order to determine the effect of increasingly intensive fertilization with potassium, applied in combination with nitrogen, on the content of trace elements in soil after the harvest of maize (Zea mays L.). The soil content of trace elements depended on the fertilization with potassium and nitrogen. Potassium fertilization had a stronger effect on the content of trace elements in the pots fertilized with the lower nitrogen dose (130 mg N kg−1 of soil). The increasing doses of potassium led to a higher soil content of zinc (Zn), and especially of nickel (Ni). The impact of potassium fertilization on the content of the remaining trace elements in the soil was less unambiguous, and depended on the dose of potassium and nitrogen fertilization. Nitrogen fertilization resulted in a higher soil content of manganese (Mn), chromium (Cr), nickel (Ni) and cadmium (Cd), as well as a decreased soil content of lead (Pb). It needs to be underlined that changes in the soil content of Ni, Cd, and Pb, effected by nitrogen fertilization, were larger than in the cases of the other trace elements. The influence of potassium and nitrogen fertilization did not result in exceeding the current threshold amounts of trace elements set for agriculturally used soil. An increase in the contents of some trace elements in soil is beneficial from an agricultural point of view. Some of these elements are necessary for the correct growth and development of arable plants.
Predominant Single Stable VpmaV Expression in Strain GM139 and Major Differences with Mycoplasma agalactiae Type Strain PG2
Authors: Barbosa Maysa Santos , Spergser Joachim , Marques Lucas Miranda , Timenetsky Jorge , Rosengarten Renate , Chopra-Dewasthaly Rohini
Published: January 2022

Although mycoplasmas have a reduced genome and no cell wall, they have important mechanisms for the antigenic variation in surface lipoproteins that modulate their interactions with the host. Mycoplasma agalactiae, the main etiological agent of contagious agalactia, has a multigene family involved in the high-frequency phase variation in surface lipoproteins called variable proteins of M. agalactiae (Vpmas). The Vpma lipoproteins are involved in the immune evasion, colonization, dissemination, and persistence of M. agalactiae in the host. In this paper, we evaluate the Vpma phenotypic profiles of two different strains of M. agalactiae, namely, GM139 and the type strain PG2, to assess possible correlations between Vpma phase variability and the geographic localization, animal origin, and pathogenicity of these two strains. Using monospecific Vpma antibodies against individual Vpmas in immunoblots, we demonstrate that, unlike PG2, which expresses six Vpma proteins with high-frequency phase variation, colonies of GM139 predominantly express VpmaV and do not exhibit any sectoring phenotype for any Vpma. Since VpmaV is one of the most important Vpmas for cell adhesion and invasion, its predominant sole expression in GM139 without high-frequency variation may be the basis of the differential pathogenicity of GM139 and PG2. Additionally, MALDI-ToF MS analysis also demonstrates significant differences between these two strains and their relatedness with other M. agalactiae strains.
Testicular Localization and Potential Function of Vimentin Positive Cells during Spermatogonial Differentiation Stages
Authors: Niazi Tabar Amirreza , Azizi Hossein , Hashemi Karoii Danial , Skutella Thomas
Published: January 2022

Vimentin is a type of intermediate filament (IF) and one of the first filaments expressed in spermatogenesis. Vimentin plays numerous roles, consisting of the determination of cell shape, differentiation, cell motility, the maintenance of cell junctions, intracellular trafficking, and assisting in keeping normal differentiating germ cell morphology. This study investigated the vimentin expression in two populations of undifferentiated and differentiated spermatogonia. We examined vimentin expression in vivo and in vitro by immunocytochemistry (ICC), immunohistochemistry (IMH), and Fluidigm real-time polymerase chain reaction. IMH data showed that the high vimentin expression was localized in the middle of seminiferous tubules, and low expression was in the basal membrane. ICC analysis of the colonies by isolated differentiated spermatogonia indicated the positive expression for the vimentin antibody, but vimentin’s expression level in the undifferentiated population was negative under in vitro conditions. Fluidigm real-time PCR analysis showed significant vimentin expression in differentiated spermatogonia compared to undifferentiated spermatogonia (p < 0.05). Our results showed that vimentin is upregulated in the differentiation stages of spermatogenesis, proving that vimentin is an intermediate filament with crucial roles in the differentiation stages of testicular germ cells. These results support the advanced investigations of the spermatogenic process, both in vitro and in vivo.
Modulation of Mucin Secretion in the Gut of Young Pigs by Dietary Threonine and Non-Essential Amino Acid Levels
Authors: Święch Ewa , Tuśnio Anna , Taciak Marcin , Barszcz Marcin
Published: January 2022

The aim of the study was to determine the effect of threonine (Thr) and non-essential amino acid (NEAA) levels on mucin secretion and sugar composition of digesta and crude mucin preparations analyzed in different segments of the gut in young pigs. A two-factorial experiment was conducted on 72 pigs using the following factors: Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible(SID) Thr/kg) and wheat gluten (WG) level used as a source of NEAA (20.4, 40.4 and 60.4 g WG protein in WG20, WG40 and WG60 diets, respectively). Mucin content was affected only by WG level. Tissue mucin content in the duodenum was higher in WG60 pigs than in WG20 and WG40 pigs, whereas in the middle jejunum was higher in WG40 and WG60 pigs than in WG20 pigs. In contrast, luminal crude mucin content in the proximal colon was lower in WG60 pigs compared to WG40 pigs. The lowest and highest Thr levels reduced arabinose and xylose contents and increased glucose content in ileal digesta. The highest WG level reduced arabinose and xylose contents and increased glucose content in ileal digesta. The lowest WG level increased mannose content in ileal digesta. WG60 level decreased the content of arabinose and galactose compared to lower WG levels in colonic digesta. Arabinose content was higher, while glucose and galactose contents were lower in crude mucin preparations isolated from colonic digesta in pigs fed diets containing the highest Thr level. The content of tissue mucin was higher in the ileum and proximal colon and lower in the duodenum than in the middle jejunum, whereas luminal mucin content was lower in the proximal colon than in the ileum. Ileal digesta contained less arabinose and glucose and more galactose as compared to colonic digesta. In conclusion, no effect of dietary Thr levels on mucin secretion in the gut of young pigs was found. Wheat gluten added to the diet with adequate Thr content positively affected mucin secretion only in the duodenum and middle jejunum.
Study of Animal Mixing and the Dynamics of Hepatitis E Virus Infection on a Farrow-to-Finish Pig Farm
Authors: Withenshaw Susan M. , Grierson Sylvia S. , Smith Richard P.
Published: January 2022

In Europe, swine are a livestock reservoir for Hepatitis E virus genotype 3 (HEV-3). Consumption of food containing HEV-3 can cause zoonotic human infection, though risk is reduced by heat treatment. Implementing controls that limit infection in slaughter pigs may further reduce foodborne transmission risk but knowledge of infection dynamics on commercial farms is limited. This study addressed this knowledge gap and in particular investigated the influence of group mixing. Faeces were collected from grower (n = 212) and fattener (n = 262) pigs on a farrow-to-finish farm on four occasions. HEV RNA was detected on all occasions, and prevalence was higher in growers (85.8%) than fatteners (26.0%; p < 0.001). HEV-positive samples were also collected from the wider farm environment (n = 67; 64.7% prevalence), indicating potential sources for HEV re-circulation within the herd. Timing of infection in a cohort was also investigated. HEV was absent from all piglet faeces (n = 98) and first detected at weaner stage (25.7% prevalence), but only in groups weaned earlier or comprising pigs from many different litters. Farrowing sow faeces (n = 75) were HEV-negative but antibodies were detected in blood from two sows. Results suggest that multiple factors influence HEV infection dynamics on pig farms, and potential foci for further study into practical control solutions are highlighted.