bluuebunny/crossref_metadata_2025 huggingface dataset:
Paraglacial Rock Slope Stability Under Changing Environmental Conditions, Safuna Lakes, Cordillera Blanca Peru
Authors: Klimeš Jan , Novotný Jan , Rapre Alejo Cochacin , Balek Jan , Zahradníček Pavel , Strozzi Tazio , Sana Hamid , Frey Holger , René Miloš , Štěpánek Petr , Meitner Jan , Junghardt Johan
Published: May 2021

Landslides or landslide-induced impact waves in high mountain lakes represent a high hazard for society, calling for realistic assessments of rock slope stability responsible for the process chain initiation. This task is often hampered by complex interplays of triggers, which effects on slope stability may be delayed by decades or even millennia, while historical records describing slope topography or landslide occurrences are usually shorter and incomplete. This article builds on rarely available detailed historical data describing the site of the 2002 rock avalanche in the Cordillera Blanca, Peru. It caused a dangerous impact wave in the Safuna Alta Lake resulting in a minor flood, but ongoing downstream development significantly increased the risk of a comparable event. Pre-2002 and post-2002 failure slope topography, 70 years long history of glaciation and landslide occurrences were combined with non-invasive field geological surveys and laboratory geotechnical analyses to characterize the distinct morphological parts of the failed slope with reliable engineering geological slope models. Slope stability was calculated for a series of environmental scenarios providing insights into the 2002 rock avalanche failure mechanism and dynamics as well as the role of glacier slope support for its stability. Results show that the rock slope stability is governed by discontinuous slip planes where rock bridges represent the most likely additional resisting forces. The effect of glacier support on the slope stability is limited under full-water saturation of the rocks and due to specific morpho-structural conditions. Importance of the long-term, progressive deterioration of the rock slope strength under paraglacial environment and repeated seismic shaking is illustrated by the fact that even the Little Ice Age maximum glacier extend only had minor positive effect on the pre-2002 rock avalanche slope stability. Despite of that, the slope remained without a major failure for decades or possibly even centuries. Its collapse in 2002 caused retrogressive movements of the adjacent slope, which remains highly unstable until now. Therefore the future safety of the lake would largely benefit from the implementation of a reliable slope movement monitoring system.
Timing and Mechanisms of the Formation of the Dark Layers in the Sea of Japan During the Last 40 kyr
Authors: Gorbarenko S. , Shi X. , Bosin A. , Liu Y. , Artemova A. , Zou J. , Yanchenko E. , Vasilenko Y. , Wu Y. , Hu L.
Published: May 2021

The marginal location of the Sea of Japan and its constrained water exchange with the western Pacific make this sea a subtle subject for the investigation of orbital and suborbital climate changes. However, the response of this unique basin to the climate and sea level changes at the end of the last glaciation and deglaciation and during the Holocene is not fully understood. We provided detailed reconstructions of the dark layers including the timing and mechanisms responsible for their formation, during the last 40°kyr, based on the multiproxy correlation of three cores from the northern and central parts of the sea with well-dated δ18O records of the Greenland ice and China cave stalagmites. High resolution color photo lightness, the conventional color parameters L* and b*, AMS 14C data, chlorin and carbonate calcium content and pollen climate parameters allowed the correlation of the DLs of these cores with Greenland interstadials (GI), Heinrich stadials (HS) and summer East Asian monsoon intensity. DLs 9, 8, 7, and 6, formed after Heinrich stadials 4 (38.5–39.5°ka), were triggered by GIs 8, 7, 6 and 5, coeval with the intensification of the East Asian summer monsoon and the increase of surface water stratification and productivity. The long-lasting GI 8, accompanied by significant climate warming, led to the formation of the more intense DL 9. The accumulation of DL five was forced by a rapid global sea level fall, coeval with cold HS 3, due to the decrease of saline Tsushima Current water input into the sea, increased surface water stratification and a drop in deep water ventilation. DL four was probably launched by GI 3 and summer East Asian monsoon intensification. Further falls in global sea level during the last glacial maximum led to the formation of DLs 3 and 2 during the periods 27.0–24.2°ka and 23.5–17.0°ka, respectively. DL 1 was associated with significant summer East Asian monsoon intensification and environmental warming at the onset of the Holocene.
Comments on the Morphology of Basal Paravian Shoulder Girdle: New Data Based on Unenlagiid Theropods and Paleognath Birds
Authors: Novas Fernando E. , Motta Matías J. , Agnolín Federico L. , Rozadilla Sebastián , Lo Coco Gastón E. , Brissón Egli Federico
Published: May 2021

In 1976 John Ostrom published an enlightening paper about the anatomical transformations in the shoulder girdle and forelimb elements along the origin of birds. Most of his ideas were based on comparingArchaeopteryx lithographicawith the extant New World vultureCathartes aura. Ostrom offered innovative ideas about range of movements and function of wing elements in the basal birdArchaeopteryx. Further, he explored anatomical transformations that may have occurred at early stages of the evolution of flight and established several hypothetical steps toward the acquisition of flapping flight in modern birds. Since then, however, our understanding of paravian diversity and anatomy has increased dramatically. Based on novel information derived from recent experiments, and currently available anatomical evidence of basal paravians, the present paper aims to review some important topics on pectoral girdle anatomy related to flight origins. Further, a brief analysis of pectoral girdle osteology and myology of the extant paleognathRhea americanais also included with the aim to test whether Ostrom’s ideas still remain valid under this new context, based on available phylogenetic and anatomical frameworks.
Feedback Between Carbon and Nitrogen Cycles During the Ediacaran Shuram Excursion
Authors: Xu Dongtao , Wang Xinqiang , Shi Xiaoying , Peng Yongbo , Stüeken Eva E.
Published: May 2021

The middle Ediacaran Period records one of the deepest negative carbonate carbon isotope (δ13Ccarb) excursions in Earth history (termed the Shuram excursion). This excursion is argued by many to represent a large perturbation of the global carbon cycle. If true, this event may also have induced significant changes in the nitrogen cycle, because carbon and nitrogen are intimately coupled in the global ocean. However, the response of the nitrogen cycle to the Shuram excursion remains ambiguous. Here, we reported high resolution bulk nitrogen isotope (δ15N) and organic carbon isotope (δ13Corg) data from the upper Doushantuo Formation in two well-preserved sections (Jiulongwan and Xiangerwan) in South China. The Shuram-equivalent excursion is well developed in both localities, and our results show a synchronous decrease in δ15N across the event. This observation is further supported by bootstrapping simulations taking into account all published δ15N data from the Doushantuo Formation. Isotopic mass balance calculations suggest that the decrease in δ15N during the Shuram excursion is best explained by the reduction of isotopic fractionation associated with water column denitrification (εwd) in response to feedbacks between carbon and nitrogen cycling, which were modulated by changes in primary productivity and recycled nutrient elements through remineralization of organic matter. The study presented here thus offers a new perspective for coupled variations in carbon and nitrogen cycles and sheds new light on this critical time in Earth history.
Promoting Students’ Scientific Habits of Mind and Chemical Literacy Using the Context of Socio-Scientific Issues on the Inquiry Learning
Authors: Wiyarsi Antuni , Prodjosantoso A. K. , Nugraheni Anggiyani R. E.
Published: May 2021

This quasi-experimental study used a pre-/posttest design to investigate student’s scientific habits of mind (SHOM) and chemical literacy. There were 32 students of the 11th grade selected for the experimental and control class. They were taken by random sampling. The experimental class applied inquiry learning via Predict-Observe-Explain-Extend (POEEd) using the socio-scientific issue (SSI) context, while the control class used a scientific approach that addressed the Indonesian national curriculum. The Acid-Base Chemical Literacy Test (A-BCLT) consisted of 19 items that used four contexts. Argumentative question added on each context. The other instrument was the SHOM scale in the SSI context. The scale consisted of 20 items in seven factors of SHOM. A group of experts looked at both instruments to ensure content validity. Then, the empirical test showed that both instruments have good reliability. MANOVA as the inferential statistic technique was used to analyze the differences among the group. The results showed that there was a significant difference in SHOM and students’ chemical literacy simultaneously. However, there was only a significant difference in students’ chemical literacy on separate analyses. Students have a better scientific argument in the case of acid rain as SSI, but it was less good for consumption of ulcer medicine for fasting people. Meanwhile, for SHOM, students have better thinking in open-mindedness and objectivity factors but less on curiosity. Some of the interesting findings and their implications are discussed in this study. The integration of SSI in chemistry learning is important. It is meaningful in promoting students’ thinking skills to become responsible citizens in the future.
A Review on Multifunctional Carbon-Dots Synthesized From Biomass Waste: Design/ Fabrication, Characterization and Applications
Authors: Khairol Anuar Nurul Kamilah , Tan Huey Ling , Lim Ying Pei , So’aib Mohamad Sufian , Abu Bakar Noor Fitrah
Published: May 2021

Carbon-Dots (C-Dots) have drawn much attention in recent years owing to their remarkable properties such as high biocompatibility, low toxicity, nano-scale size, and ease of modification with good tuneable photoluminescence performance. These unique properties have led C-Dots to become a promising platform for bioimaging, metal ion sensing and an antibacterial agent. C-Dots can be prepared using the top-down and bottom-up approaches, in which the latter method is commonly used for large scale and low-cost synthesis. C-Dots can be synthesized using sustainable raw materials or green biomass since it is environmentally friendly, in-expensive and most importantly, promotes the minimization of waste production. However, using biomass waste to produce high-quality C-Dots is still a matter of concern waiting for resolution, and this will be the main focus of this review. Fundamental understanding of C-Dots such as structure analysis, physical and chemical properties of C-Dots, various synthesis methodology and type of raw materials used are also discussed and correlated comprehensively. Additionally, factors affecting the bandgap of the C-Dots and the strategies to overcome these shortcomings are also covered. Moreover, formation mechanism of C-Dots focusing on the hydrothermal method, option and challenges to scale up the C-Dots production are explored. It is expected that the great potential of producing C-Dots from agricultural waste a key benefit in view of their versatility in a wide range of applications.
Effects of Flower-Enriched Ecological Focus Areas on Functional Diversity Across Scales
Authors: Nilsson Lovisa , Klatt Björn K. , Smith Henrik G.
Published: May 2021

Ecological Focus Areas (EFAs) to benefit biodiversity became mandatory in intensively farmed landscapes after the reform of the European Common Agricultural Policy (CAP) in 2013. The implementation of EFAs as uncropped field margins has been criticized as ineffective but created a window of opportunity to test if augmenting them with annual flower strips can benefit biodiversity. In this study, we investigated if annual flower strips on EFAs benefited functional biodiversity in intensively farmed landscapes. To this end we established eleven annual flower strips with a seed mixture targeted for both natural enemies and pollinators, on areas were farmers had planned for EFAs. We determined effects on aphids and their natural enemies in cereal fields close to six of the flower strips, and for solitary bees and wasp close to and in the surroundings of all eleven flower strips. We found that annual flower strips benefited the abundance of hoverfly larvae and possibly also that of solitary bees. However, there were neither any significant effects on natural enemies (other than hoverfly larvae), nor any difference in natural pest control as shown by lack of differences in aphid numbers and parazitation rates. Abundances of solitary bees and wasps in the surrounding landscapes were unaffected, although there was a tendency for more solitary bee cells closer to the strips. We suggest that the critical issue leading to the mostly negative results is the lack of permanent structures to sustain populations of arthropods that in turn can benefit from annual flower strips. Hence, future agri-environmental policies need to carefully consider if and how annual agri-environmental measures should be implemented in intensively managed agricultural landscapes, e.g., by combining them with more permanent structures.
Rhodobacter azotoformans LPS (RAP99-LPS) Is a TLR4 Agonist That Inhibits Lung Metastasis and Enhances TLR3-Mediated Chemokine Expression
Authors: Murakami Kaoru , Kamimura Daisuke , Hasebe Rie , Uchida Mona , Abe Nobuya , Yamamoto Reiji , Jiang Jing-Jing , Hidaka Yasuhiro , Nakanishi Yuko , Fujita Shuzo , Toda Yuki , Toda Nobuhiro , Tanaka Hiroki , Akira Shizuo , Tanaka Yuki , Murakami Masaaki
Published: May 2021

The lipopolysaccharides (LPSs) ofRhodobacterare reported to be TLR4 antagonists. Accordingly, the extract ofRhodobacter azotoformans(RAP99) is used as a health supplement for humans and animals in Japan to regulate immune responsesin vivo. We previously analyzed the LPS structure of RAP99 (RAP99-LPS) and found it is different from that ofE. coli-LPS but similar to lipid A fromRhodobacter sphaeroides(RSLA), a known antagonist of TLR4, with both having three C14 fatty acyl groups, two C10 fatty acyl groups, and two phosphates. Here we show that RAP99-LPS has an immune stimulatory activity and acts as a TLR4 agonist. Pretreatment of RAP99-LPS suppressedE. coli-LPS-mediated weight loss, suggesting it is an antagonist againstE. coli-LPS like other LPS isolated fromRhodobacter. However, injections of RAP99-LPS caused splenomegaly and increased immune cell numbers in C57BL/6 mice but not in C3H/HeJ mice, suggesting that RAP99-LPS stimulates immune cellsviaTLR4. Consistently, RAP99-LPS suppressed the lung metastasis of B16F1 tumor cells and enhanced the expression of TLR3-mediated chemokines. These results suggest that RAP99-LPS is a TLR4 agonist that enhances the activation status of the immune system to promote anti-viral and anti-tumor activityin vivo.
Microbiota and Microglia Interactions in ASD
Authors: Davoli-Ferreira Marcela , Thomson Carolyn A. , McCoy Kathy D.
Published: May 2021

Autism spectrum disorders (ASD) are serious, highly variable neurodevelopmental disorders, commonly characterized by the manifestation of specific behavioral abnormalities, such as stereotypic behaviors and deficits in social skills, including communication. Although the neurobiological basis for ASD has attracted attention in recent decades, the role of microglial cells, which are the main resident myeloid cell population in the brain, is still controversial and underexplored. Microglia play several fundamental roles in orchestrating brain development and homeostasis. As such, alterations in the intrinsic functions of these cells could be one of the driving forces responsible for the development of various neurodevelopmental disorders, including ASD. Microglia are highly sensitive to environmental cues. Amongst the environmental factors known to influence their intrinsic functions, the gut microbiota has emerged as a central player, controlling both microglial maturation and activation. Strikingly, there is now compelling data suggesting that the intestinal microbiota can play a causative role in driving the behavioural changes associated with ASD. Not only is intestinal dysbiosis commonly reported in ASD patients, but therapies targeting the microbiome can markedly alleviate behavioral symptoms. Here we explore the emerging mechanisms by which altered microglial functions could contribute to several major etiological factors of ASD. We then demonstrate how pre- and postnatal environmental stimuli can modulate microglial cell phenotype and function, underpinning the notion that reciprocal interactions between microglia and intestinal microbes could play a crucial role in ASD aetiology.
Improvement of Neoantigen Identification Through Convolution Neural Network
Authors: Hao Qing , Wei Ping , Shu Yang , Zhang Yi-Guan , Xu Heng , Zhao Jun-Ning
Published: May 2021

Accurate prediction of neoantigens and the subsequent elicited protective anti-tumor response are particularly important for the development of cancer vaccine and adoptive T-cell therapy. However, current algorithms for predicting neoantigens are limited by in vitro binding affinity data and algorithmic constraints, inevitably resulting in high false positives. In this study, we proposed a deep convolutional neural network named APPM (antigen presentation prediction model) to predict antigen presentation in the context of human leukocyte antigen (HLA) class I alleles. APPM is trained on large mass spectrometry (MS) HLA-peptides datasets and evaluated with an independent MS benchmark. Results show that APPM outperforms the methods recommended by the immune epitope database (IEDB) in terms of positive predictive value (PPV) (0.40 vs. 0.22), which will further increase after combining these two approaches (PPV = 0.51). We further applied our model to the prediction of neoantigens from consensus driver mutations and identified 16,000 putative neoantigens with hallmarks of ‘drivers’.
Vulnerable Marine Ecosystems, Communities, and Indicator Species: Confusing Concepts for Conservation of Seamounts
Authors: Watling Les , Auster Peter J.
Published: May 2021

The concept of “vulnerable marine ecosystem” (VME) was included in United Nations (UN) General Assembly Resolution 61/105 as part of an international effort to minimize the effects of seafloor fisheries on the high seas. However, defining a VME was left to be developed by technical guidance to the UN Food and Agricultural Organization. In that venue certain categories of organisms were deemed to be indicator species, suggesting that areas with those species would be considered VMEs with subsequent management measures implemented to conserve those ecosystem attributes. We note that on seamounts VME indicator species can be distributed widely, in dense clusters or sparsely. A dense cluster, for example, of scleractinian corals or sponges, is most often referred to as a VME, but we argue that any such dense cluster is not an ecosystem, rather it is a community, likely one of many that make up the ecosystem. Other communities on the seamount that are not part of that dense cluster could include many small species (some yet to be discovered) who are also part of the ecosystem because they are part of the web of interactions and flow of materials/energy on the seamount. We also suggest that a seamount ecosystem might extend over several or many seamounts in a biogeographic area. We conclude that the term “ecosystem” in the VME concept outlined by the United Nations needs to be re-evaluated from a classical ecological perspective leading to spatial management approaches that better address ecologically relevant space and time scales.
The Effect of the Deformity Genetic Background of the Breeders on the Spawning Quality of Gilthead Seabream (Sparus aurata L.)
Authors: Lorenzo-Felipe Álvaro , Shin Hyun Suk , León-Bernabeu Sergio , Pérez-García Cathaysa , Zamorano María Jesús , Pérez-Sánchez Jaume , Afonso-López Juan Manuel
Published: May 2021

Fish egg quality is strongly related with the ability of the egg to be fertilized and develop a normal embryo with good survival and a lack of abnormalities. Large variations in the spawning quantity or quality impact directly in the competitiveness and sustainability of hatcheries, which create an overly large broodstock in order to satisfy the on-growing companies’ demand for undeformed fry. The present study reports, for the first time in relation to gilthead seabream, the effect of the genetic background of breeders for presence or absence of deformity on their spawning quality and the importance of considering this when creating broodstock. The spawning quality of crosses of breeders with genetic background for presence or absence of deformity (EBVdef), were evaluated during a whole spawning season, through study of the following traits: oocyte yield, fertilization rate, viability rate, hatching rate, larval survival rate, fertilized eggs, viable eggs, hatched eggs, and number of alive larvae. Breeders with a genetic background for deformity and a normal phenotype had shorter spawning periods, lower oocyte yield and, consequently, produced a lower number of alive larvae. In these two traits, the genetic background of breeders was of greater importance during intermediate spawning periods, when spawning is generally considered optimal for the industry, while environmental factors were more important at the beginning and end of the spawning season. In conclusion, these results demonstrate the importance of controlling the breeders’ genetics when creating broodstock.
Interactions Between Ultraviolet B Radiation, Warming, and Changing Nitrogen Source May Reduce the Accumulation of Toxic Pseudo-nitzschia multiseries Biomass in Future Coastal Oceans
Authors: Kelly Kyla J. , Fu Fei-Xue , Jiang Xiaowen , Li He , Xu Dong , Yang Nina , DeMers Michelle A. , Kling Joshua D. , Gao Kunshan , Ye Naihao , Hutchins David A.
Published: May 2021

Understanding the environmental conditions that trigger Pseudo-nitzschia bloom formation and domoic acid (DA) production is critical as the frequency and severity of these toxic blooms increases in the face of anthropogenic change. However, predicting the formation of these harmful blooms in a future ocean remains a challenge. Previous studies have examined the effects of single environmental drivers on Pseudo-nitzschia spp. growth and toxin production, but few have considered the interactions between them. In this multiple driver study with Pseudo-nitzschia multiseries, we used a full factorial matrix experimental design to examine the simultaneous effects of temperature (20 and 25°C), nitrogen source (nitrate and urea), and irradiance (photosynthetically active radiation with and without ultraviolet B radiation; UVB). This strain of P. multiseries was unable to withstand prolonged exposures (>0.5 h) to 0.06 mw⋅cm–2 UVB light, with implications for near-surface bloom formation if future shallower mixed layers increase UVB exposure. Growth rates were inhibited by UVB, but photosynthesis and carbon fixation continued at a reduced capacity. Additionally, DA synthesis continued despite UVB-induced growth inhibition. Warming by 5°C enhanced cellular DA quotas three-fold. Within these warmer treatments, urea-grown cultures exposed to UVB had the highest amount of DA per cell, suggesting that interactive effects between UVB exposure, warming, and urea can synergistically enhance toxin production. However, overall production of toxic biomass was low, as growth-integrated DA production rates were near zero. This indicates that although Pseudo-nitzschia multiseries cell-specific toxicity could worsen in an anthropogenically-altered future ocean, bloom formation may be inhibited by increased exposure to UVB. This multi-variable experimental approach revealed previously unknown interactions that could not have been predicted based on combined effects of single-variable experiments. Although P. multiseries DA production may be enhanced in a future ocean, inherent sensitivity to prolonged UVB exposure may moderate trophic transfer of toxin to coastal food webs.
Drivers of Spatial Distributions of Basking Shark (Cetorhinus maximus) in the Southwest Pacific
Authors: Finucci Brittany , Duffy Clinton A. J. , Brough Tom , Francis Malcolm P. , Milardi Marco , Pinkerton Matthew H. , Petersen Grady , Stephenson Fabrice
Published: May 2021

Basking sharks (Cetorhinus maximus) were widely reported throughout New Zealand waters. Once commonly observed, and sometimes in large numbers, basking sharks are now infrequently reported. Basking shark observations are known to be highly variable across years, and their distribution and occurrence have been shown to be influenced by environmental predictors such as thermal fronts, chl-a concentration, and the abundance of prey (zooplankton). Little is known of basking sharks in the South Pacific and more information on distribution, habitat use, and migratory patterns is required to better understand the species’ regional ecology. Here, we used bootstrapped Habitat Suitability Models [HSM, ensembled from Boosted Regression Tree (BRT) and Random Forest (RF) models] to determine the drivers of basking shark distribution, predict habitat suitability and estimated uncertainty in the South Pacific for the first time. High−resolution environmental (1 km2 grid resolution) and biotic data, including inferred prey species, and all available basking shark records across New Zealand’s Exclusive Economic Zone (EEZ) were included in the ensemble HSMs. The most influential driver of modeled basking shark distribution was vertical flux of particulate organic matter at the seabed, which may indicate higher levels of primary production in the surface ocean and higher prey density in the mesopelagic zone and at the seafloor. The BRT and RF models had good predictive power (AUC and TSS > 0.7) and both models performed similarly with low variability in the model fit metrics. Areas of high basking shark habitat suitability included the east and west coasts of the South Island, Puysegur Ridge, and Auckland Island slope. The outputs produced here could be incorporated into future management framework for assessing threat and conservation needs (e.g., spatially explicit risk assessment) for this regionally protected species, as well as providing guidance for future research efforts (e.g., areas of interest for sampling).
Spatial Dynamics Modeling of Small-Scale Fishing Fleets With a Random Walk Approach
Authors: Quijano Quiñones Daniel R. , López-Rocha Jorge A. , Hernández-Herrera Isis , Torres-Irineo Edgar
Published: May 2021

The understanding of fishing effort allocation has been recognized as a key feature within the spatial management planning of fisheries. In small-scale fisheries this assessment is challenging because of their multi-specific context. This has resulted in management regulations that do not fulfill their objectives. Therefore, the analysis of vessels movement is needed for the better assessment and management of small-scale fisheries. Thus, we used a Random Walk (RW) modeling approach to characterize the fishing trips of small-scale multi-gear vessels targeting multiple species. We analyzed activities of three different gears (hand net, handline, longline) and two fishing methods (diving, “gareteo”) recorded with a GPS from 156 fishing trips along three fishing seasons (2018–2020) by the small-scale fleet of Yucatan, Mexico. We fitted seven RW models for the movement data and compared their relative and absolute fit through a delta Akaike test and G-Tests respectively. A total of 143 trajectories showed conclusive evidence to establish a CCRW-type movement; the remaining 13 trajectories fit three models (CCRW, TRW and CRW). The Random Walk models were useful for modeling a multi-gear, small-scale fleet operating in southeast Mexico. According to our results, the Composite Correlated Model was the most suitable RW for this fleet. For future studies, we suggest increasing the number of fishing trips and conducting specific studies by gear and fishing method. Also, the application of relative and absolute fit tests to compare the performance of RW models is highly recommended.
Flexural Behavior of Simply Supported Beams Consisting of Gradient Concrete and GFRP Bars
Authors: Ji Jing , Zhang Runbao , Yu Chenyu , He Lingjie , Ren Hongguo , Jiang Liangqin
Published: May 2021

In order to study the flexural behavior of simply supported beams consisting of gradient concrete and GFRP bars, 28 simply supported beams were designed. The main parameters included the strength grades of high-strength concrete (HSC), GFRP reinforcement ratio, and sectional height of HSC. Based on nonlinear constitutive models of materials, meanwhile, considering the bond slip between concrete and GFRP bars, five simply supported beams with gradient concrete and five simply supported beams with GFRP bars were simulated, respectively. Then the mid-span load–displacement curves of beams were obtained. By comparing with the experimental data, the rationality of material constitutive models and finite element modeling was verified. Based on this, the parameter analysis of the beams with GFRP bars and gradient concrete was carried out, and the failure modes of the beams were obtained through investigation. The results show that the failure process of the beams can be divided into two stages: elastic stage and working stage with cracks. With the increase of GFRP reinforcement ratio, the flexural bearing capacity of the beams does not change significantly, while their stiffness increases gradually. The flexural bearing capacity of the beams can be significantly improved by appropriately increasing the strength and sectional height of HSC. The ultimate bearing capacity of the beams is 40% higher than that of the GFRP concrete beams. Finally, based on the plane-section assumption, the calculation formula of normal-section flexural bearing capacity of this kind of beams is proposed through statistical regression method.
The Detective Value of Magnetically Controlled Robotic Capsule Endoscopy in Patients With Suspected Small Intestinal Disease
Authors: Chen Xiao-Yu , Da Wei , Liang Rui , Fan Hui-Ning , Yi You-Cai , Chen Ming , Qin Huang-Wen , Zhang Jing , Zhu Jin-Shui
Published: May 2021

Objective: To explore the detective value of magnetically controlled robotic capsule endoscopy (MCRCE) in patients with suspected small intestinal disease.

Patients and Methods: In total, 1,802 patients with suspected small intestinal disease and negative gastroenteroscopy from Shanghai Jiao Tong University Affiliated Sixth People's Hospital were examined with MCRCE, and the data were collected for further analysis.

Results: Among the 1,802 patients who were examined with MCRCE, 974 were diagnosed with small intestinal disease, reaching a positive detection rate of 54.1%. The five most common conditions that were detected include non-specific enteritis in 722 cases (40.1%), small intestinal ulcers in 87 cases (4.8%), abnormal small bowel evacuation in 45 cases (2.5%), small intestinal bleeding in 33 cases (1.8%), and small intestinal yellow spots in 31 cases (1.7%). The running time of the capsules in the small intestine ranged from 85–437 min, with an average of 210.24 ± 89.08 min. No complications, such as intestinal obstruction or capsule retention, were observed in all patients.

Conclusion: MCRCE is a safe and non-invasive endoscopic examination with a highly accurate detection rate for small intestinal diseases.
Cardiorenal Fat: A Cardiovascular Risk Factor With Implications in Chronic Kidney Disease
Authors: D'Marco Luis , Puchades María Jesús , Panizo Nayara , Romero-Parra María , Gandía Lorena , Giménez-Civera Elena , Pérez-Bernat Elisa , Gonzalez-Rico Miguel , Gorriz José Luis
Published: May 2021

There is a growing interest in the potential role of adipose tissues in cardiac and renal pathophysiology, and determining the mechanisms by which fat compartments around the heart and kidneys influence cardiovascular disease is of clinical importance in both general and high-risk populations. Epicardial fat and perirenal fat have been associated with adverse outcomes in chronic kidney disease (CKD) patients. Epicardial fat is a rich source of free fatty acids and is capable of secreting inflammatory and pro-atherogenic cytokines that promote atherosclerosis through a local paracrine effect. Recent evidence has demonstrated that perirenal fat has a closer correlation with kidney diseases than other visceral fat deposits in obesity or metabolic disturbances. Moreover, perirenal fat has been reported as an independent risk factor for CKD progression and even associated with cardiorenal dysfunction. Accordingly, these forms of organ-specific fat deposits may act as a connecter between vascular and cardiorenal disease. This review explores the possible links between epicardial and perirenal fat and its significant role as a modulator of cardiorenal dysfunction in CKD patients.
Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both
Authors: Pérez Montserrat , Robres Pilar , Moreno Bernardino , Bolea Rosa , Verde Maria T. , Pérez-Laguna Vanesa , Aspiroz Carmen , Gilaberte Yolanda , Rezusta Antonio
Published: May 2021

Background: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models.

Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing.

Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study.

Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus.

Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected.
Mining of Gram-Negative Surface-Active Enzybiotic Candidates by Sequence-Based Calculation of Physicochemical Properties
Authors: Vázquez Roberto , Blanco-Gañán Sofía , Ruiz Susana , García Pedro
Published: May 2021

Phage (endo)lysins are nowadays one of the most promising ways out of the current antibiotic resistance crisis. Either as sole therapeutics or as a complement to common antibiotic chemotherapy, lysins are already entering late clinical phases to get regulatory agencies’ authorization. Even the old paradigm of the inability of lysins to attack Gram-negative bacteria from without has already been overcome in a variety of ways: either by engineering approaches or investigating the natural mechanisms by which some wild-type lysins are able to interact with the bacterial surface. Such inherent ability of some lysins has been linked to antimicrobial peptide (AMP)-like regions, which are, on their own, a significant source for novel antimicrobials. Currently, though, many of the efforts for searching novel lysin-based antimicrobial candidates rely on experimental screenings. In this work, we have bioinformatically analyzed the C-terminal end of a collection of lysins from phages infecting the Gram-negative genus Pseudomonas. Through the computation of physicochemical properties, the probability of such regions to be an AMP was estimated by means of a predictive k-nearest neighbors (kNN) model. This way, a subset of putatively membrane-interacting lysins was obtained from the original database. Two of such candidates (named Pae87 and Ppl65) were prospectively tested in terms of muralytic, bacteriolytic, and bactericidal activity. Both of them were found to possess an activity against Pseudomonas aeruginosa and other Gram-negative bacterial pathogens, implying that the prediction of AMP-like regions could be a useful approach toward the mining of phage lysins to design and develop antimicrobials or antimicrobial parts for further engineering.
Prevalence of 16S rRNA Methylation Enzyme Gene armA in Salmonella From Outpatients and Food
Authors: Lu Xin , Zeng Mei , Zhang Ning , Wang Mengyu , Gu Baoke , Li Jiaqi , Jin Huiming , Xiao Wenjia , Li Zhe , Zhao Hongqun , Zhou Haijian , Li Zhenpeng , Xu Jialiang , Xu Xuebin , Kan Biao
Published: May 2021

Salmonella is the primary cause of community-acquired foodborne infections, so its resistance to antimicrobials, such as aminoglycosides, is a public health issue. Of concern, aminoglycoside resistance in Salmonella is increasing rapidly. Here, we performed a retrospective study evaluating the prevalence of Salmonella harboring armA-mediated aminoglycoside resistance in community-acquired infections and in food or environmental sources. The prevalence rates of armA-harboring Salmonella strains were 1.1/1,000 (13/12,095) and 8.7/1,000 (32/3,687) in outpatient and food/environmental isolates, respectively. All the armA-harboring Salmonella strains were resistant to multiple drugs, including fluoroquinolone and/or extended-spectrum cephalosporins, and most (34/45) belonged to serovar Indiana. The armA gene of these strains were all carried on plasmids, which spanned five replicon types with IncHI2 being the dominant plasmid type. All the armA-carrying plasmids were transferable into Escherichia coli and Acinetobacter baumannii recipients. The conjugation experiment results revealed that the armA-harboring S. Indiana strains had a relatively higher ability to acquire armA-carrying plasmids. The low similarity of their pulsed field gel electrophoresis patterns indicates that the armA-harboring Salmonella strains were unlikely to have originated from a single epidemic clone, suggesting broad armA spread. Furthermore, the genetic backgrounds of armA-harboring Salmonella strains isolated from outpatients exhibited higher similarity to those isolated from poultry than to those isolated from swine, suggesting that poultry consumption maybe an infection source. These findings highlight an urgent need to monitor the prevalence and transmission of armA-harboring Salmonella, especially S. Indiana, to better understand the potential public health threat and prevent the further spread of these strains.
Geographical Distribution of Iron Redox Cycling Bacterial Community in Peatlands: Distinct Assemble Mechanism Across Environmental Gradient
Authors: Yang Liang , Jiang Ming , Zou Yuanchun , Qin Lei , Chen Yingyi
Published: May 2021

Microbial-mediated iron (Fe) oxidation and reduction greatly contribute to the biogeochemistry and mineralogy of ecosystems. However, knowledge regarding the composition and distribution patterns of iron redox cycling bacteria in peatlands remains limited. Here, using high-throughput sequencing, we compared biogeographic patterns and assemblies of the iron redox cycling bacterial community between soil and water samples obtained from different types of peatland across four regions in Northeast China. A total of 48 phylotypes were identified as potential iron redox bacteria, which had greater than 97% similarity with Fe(II)-oxidizing bacteria (FeOB) and Fe(III)-reducing bacteria (FeRB). Among them, Rhodoferax, Clostridium, Geothrix, Sideroxydans, Geobacter, Desulfovibrio, and Leptothrix could be used as bioindicators in peatlands for characterizing different hydrological conditions and nutrient demands. Across all samples, bacterial communities associated with iron redox cycling were mainly affected by pH, dissolved organic carbon (DOC), and Fe2+. Distance–decay relationship (DDR) analysis indicated that iron redox cycling bacterial communities in soil, but not in water, were highly correlated with geographic distance. Additionally, null model analysis revealed that stochastic processes substituted deterministic processes from minerotrophic fens to ombrotrophic bogs in soils, whereas deterministic processes were dominant in water. Overall, these observations suggest that bacteria involved in iron redox cycling are widespread in diverse habitats and exhibit distinct patterns of distribution and community assembly mechanisms between soil and water in peatlands.
Nucleic Acid–Based Therapeutics in Orphan Neurological Disorders: Recent Developments
Authors: Khorkova Olga , Hsiao Jane , Wahlestedt Claes
Published: May 2021

The possibility of rational design and the resulting faster and more cost-efficient development cycles of nucleic acid–based therapeutics (NBTs), such as antisense oligonucleotides, siRNAs, and gene therapy vectors, have fueled increased activity in developing therapies for orphan diseases. Despite the difficulty of delivering NBTs beyond the blood–brain barrier, neurological diseases are significantly represented among the first targets for NBTs. As orphan disease NBTs are now entering the clinical stage, substantial efforts are required to develop the scientific background and infrastructure for NBT design and mechanistic studies, genetic testing, understanding natural history of orphan disorders, data sharing, NBT manufacturing, and regulatory support. The outcomes of these efforts will also benefit patients with “common” diseases by improving diagnostics, developing the widely applicable NBT technology platforms, and promoting deeper understanding of biological mechanisms that underlie disease pathogenesis. Furthermore, with successes in genetic research, a growing proportion of “common” disease cases can now be attributed to mutations in particular genes, essentially extending the orphan disease field. Together, the developments occurring in orphan diseases are building the foundation for the future of personalized medicine. In this review, we will focus on recent achievements in developing therapies for orphan neurological disorders.
Aberrant Methylation of Aging-Related Genes in Asthma
Authors: Yang Yu , Yuan Lin , Yang Ming , Du Xizi , Qin Ling , Wang Leyuan , Zhou Kai , Wu Mengping , He Ruoxi , Feng Juntao , Xiang Yang , Qu Xiangping , Liu Huijun , Qin Xiaoqun , Liu Chi
Published: May 2021

Background: Asthma is a complex pulmonary inflammatory disease which is common among older adults. Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Nonetheless, the underlying mechanism by which differenced aging-related gene contributes to asthma pathology remains unclear. Of note, DNA methylation (DNAm) has been proven to play a critical mechanism for age-related gene expression changes. However, the methylation changes of aging-related genes in asthma patients are still obscure.

Methods: First, changes in DNAm and gene expression were detected with multiple targeted bisulfite enrichment sequencing (MethTarget) and qPCR in peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients. Second, the correlation between the DNAm levels of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Last, the receiver operator characteristic (ROC) curve and principal component analysis (PCA) were used to identify the feasibility of the candidate CpG sites as biomarkers for asthma.

Results: Compared with HCs, there was a differential mRNA expression for nine aging-related genes in peripheral blood of asthma patients. Besides, the methylation levels of the nine aging-related genes were also altered in asthma patients, and a total of 68 CpG sites were associated with the severity of asthma. Notably, 9 of the 68 CpG sites were significantly associated with pulmonary function parameters. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites (DMSs) can be used as potential biomarkers for asthma.

Conclusions: In summary, this study confirmed the differentially expressed mRNA and aberrant DNAm level of aging-related genes in asthma patients. DMSs are associated with the clinical evaluation indicators of asthma, which indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarkers for asthma.
How Quality Control Systems AID Sec-Dependent Protein Translocation
Authors: Jiang Chen , Wynne Max , Huber Damon
Published: May 2021

Graphical Abstract

Overview of the AID quality control pathways.
The Effectiveness of Music Therapy on Hand Function in Patients With Stroke: A Systematic Review of Randomized Controlled Trials
Authors: Huang Wen-Hao , Dou Zu-Lin , Jin Hui-Min , Cui Ying , Li Xin , Zeng Qing
Published: May 2021

Objective: This study aims to evaluate the efficacy of music-supported therapy for stroke patients' hand function.

Methods: The databases used included Cumulative Index to Nursing and Allied Health Literature (CINAHL), MEDLINE, PubMed, Embase, Music Index, and Google Scholar. Studies published between January 2010 and August 2020 were included. The searching key terms included “music-supported therapy,” “music therapy,” “hand function,” “hand dysfunction,” “stroke,” “ischemic,” and “hemorrhagic.” Randomized controlled trials or controlled trials involving adults who have hand function problems caused by stroke are included in this study. The methodological quality and risk of bias of the included studies were rated by two independent assessors under the guidance of Cochrane collaboration's risk of bias tool.

Results: Twelve studies that met the inclusion criteria were included in this study. Totally, the data included 598 stroke patients (345 male, 253 female) with recruited time from 1.7 months to 3 years, and the mean age of the participants were 61.09 years old. Based on the Cochrane risk of bias tool, study quality ranged from three to seven out of seven points. Compared with the control group, outcomes including hand strength, range of joint motion, dexterity of hands, arm function, and quality of life were significantly superior with music-supported therapy. Five studies reported improved dexterity of hands, and one study reported the improvement of range of motion and strength of patients' hands, which supported the therapy has positive effects on patients' hand function and improving their quality of life after the therapy. The therapy ranged over a period of 4–8 weeks, with an average duration of 30 min/session and an average of three times per week.

Conclusion: Based on the results, music-supported therapy could be a useful treatment for improving hand function and activities of daily living in patients with stroke, especially for patients within 6 months after stroke. However, the low certainty of evidence downgrades our confidence to practice in hospital. More and more randomized controlled trials and larger sample sizes are required for a deeper review.