bluuebunny/crossref_metadata_2025 huggingface dataset:
Pre-diagnosis Dietary One-Carbon Metabolism Micronutrients Consumption and Ovarian Cancer Survival: A Prospective Cohort Study
Authors: Xu He-Li , Gong Ting-Ting , Liu Fang-Hua , Wei Yi-Fan , Chen Hong-Yu , Yan Shi , Zhao Yu-Hong , Gao Song , Jiao Yi-Sheng , Wu Qi-Jun
Published: April 2022

Background and Aims

Epidemiological evidence on the relation between one-carbon metabolism (OCM) micronutrients intake and ovarian cancer (OC) survival are limited and conflicting. We evaluated the aforementioned associations in a prospective cohort-the Ovarian Cancer Follow-Up Study.

Methods

A total of 635 newly diagnosed OC patients aged 18–79 y were enrolled in the present study. Dietary intake related to one-carbon metabolism, including methionine, vitamins B2, B3, B6, B9, B12, choline, and betaine, was assessed using a validated 111-item food frequency questionnaire. Deaths were ascertained until March 31, 2021, via medical records and active follow-up. Cox proportional hazards regression model was used to evaluate the hazard ratios (HRs) and 95% confidence intervals (CIs) for these aforementioned associations.

Results

During a median follow-up of 37.2 months (interquartile: 24.7–50.2 months), 114 deaths were identified. We observed an improved survival with the highest compared with the lowest tertile of dietary vitamin B6 (HR = 0.52, 95%CI: 0.32–0.84, P-trend <0.05) and choline intake (HR = 0.50, 95%CI: 0.30–0.83, P-trend <0.05). No significant associations with OC survival were observed for dietary vitamins B2, B3, B9, B12, methionine, and betaine intake. We also observed a curvilinear association between vitamin B6 intake and OC survival (P non-linear <0.05).

Conclusion

Our study suggests that pre-diagnosis higher intake of vitamin B6 and choline may improve OC survival. Further clarification of these associations is warranted.
Protective Roles of Apigenin Against Cardiometabolic Diseases: A Systematic Review
Authors: Xu Yajie , Li Xue , Wang Hui
Published: April 2022

Apigenin is a flavonoid with antioxidant, anti-inflammatory, and anti-apoptotic activity. In this study, the potential effects of apigenin on cardiometabolic diseases were investigated in vivo and in vitro. Potential signaling networks in different cell types induced by apigenin were identified, suggesting that the molecular mechanisms of apigenin in cardiometabolic diseases vary with cell types. Additionally, the mechanisms of apigenin-induced biological response in different cardiometabolic diseases were analyzed, including obesity, diabetes, hypertension and cardiovascular diseases. This review provides novel insights into the potential role of apigenin in cardiometabolic diseases.
Exploring Trade-Offs Between Profit, Yield, and the Environmental Footprint of Potential Nitrogen Fertilizer Regulations in the US Midwest
Authors: Mandrini German , Pittelkow Cameron Mark , Archontoulis Sotirios , Kanter David , Martin Nicolas F.
Published: April 2022

Multiple strategies are available that could reduce nitrogen (N) fertilizer use in agricultural systems, ranging from voluntary adoption of new N management practices by farmers to government regulations. However, these strategies have different economic and political costs, and their relative effectiveness in decreasing N leaching has not been evaluated at scale, particularly concerning potential trade-offs in crop yield and profitability. To inform policy efforts in the US Midwest, we quantified the effects of four policy scenarios designed to reduce fertilizer N inputs without sacrificing maize yields below 95%. A simulated dataset for economically optimum N rates and corresponding leaching losses was developed using a process-based crop model across 4,030 fields over 30 years. Policy scenarios were (1) higher N prices, (2) N leaching fee, (3) N balance fee, and (4) voluntary reduction of N use by farmers, each implemented under a range of sub-levels (low to high severity). Aggregated results show that all policies decreased N rates and N leaching, but this was associated with an exponential increase in economic costs. Achieving an N leaching reduction target of 20% has an estimated pollution control cost of 30–37 US$/ha, representing 147 million US$/year when scaled up to the state level, which is in the range of current government payments for existing conservation programs. Notably, such control of N losses would reduce the environmental impact of agriculture on water quality (externalities) by an estimated 524 million US$/year, representing an increase in society welfare of 377 million US$/year. Among the four policies, directly charging a fee on N leaching helped mitigate economic losses while improving the point source reduction effect (i.e., targeting fields that were leaching hotspots) and better internalization effect (i.e., targeting fields with higher environmental impact costs). This study provides actionable data to inform the development of cost-effective N fertilizer regulations by integrating changes in crop productivity and N losses in economic terms at the field level.
Quality Evolution and Aroma Profile of Pointed Cabbage in Different Storage Regimes
Authors: Janssens Maxime , Verlinden Bert E. , Hertog Maarten L. A. T. M. , Nicolaï Bart M.
Published: April 2022

With its increasing popularity, the need for optimal storage conditions of pointed cabbages becomes more important to meet the year-round demand. Storage of the pointed varieties, however, is more difficult compared to the traditional, round varieties and is limited to a few weeks in normal air. Pointed cabbages are more susceptible to quality loss (shriveling, yellowing of leaves, weight loss, fungal, and bacterial infections) and tend to spoil much faster. In order to provide a year-round availability of the fresh product, storage under controlled atmosphere (CA) could offer a solution. In this study, pointed, white cabbage heads (Brassica oleraceavar. capitata for.albaL. subv.Conicacv. ‘Caraflex’) were stored at 1°C from November 2018 to May 2019 under four different CA conditions (1 kPa O2+ 1.5 kPa CO2, 1 kPa O2+ 5 kPa CO2, 3 kPa O2+ 1.5 kPa CO2, and 3 kPa O2+ 5 kPa CO2), and compared to storage under normal air. Results showed that CA storage resulted in a prolonged storage life with a good quality retention for both texture and aroma. CA-stored cabbages showed less weight loss, shriveling, and yellowing. Internal quality parameters [color, soluble solids content (SSC)] were stable over the whole storage period for all objects. The aroma profiles of both the storage atmosphere and cabbage samples were impacted by storage duration. The aroma of cabbage juice was also affected by the storage regime. A clear separation was found for cabbage stored under CA compared to the reference group. From the CA-treatments studied, a combination of low oxygen (1 kPa O2) and elevated carbon dioxide levels (5 kPa CO2) showed the best results maintaining quality. Storage under CA resulted in a better resemblance to the aroma of freshly, harvested produce compared to cabbages stored in normal air.
Perceived Organizational Democracy and Associated Factors: A Focused Systematic Review Based on Studies in Turkey
Authors: Geçkil Tahsin
Published: April 2022

This review study provides an opportunity to look at the level of organizational democracy (OD) that a large sample of private- and public-sector employees in an emerging market (Turkey) perceive. The focused systematic review includes empirical studies examining employees' level of OD and associated work and organizational psychological variables, using the Organizational Democracy Scale (ODS) in Turkey. This paper includes studies published between January 2014 and April 2021 in the Google Academic, Dergipark, and Ulakbim databases and on the Turkish National Thesis Center website. From a total of 1,778 records, 37 empirical studies meeting the inclusion criteria were included (with a total of N = 10,370 employees). Of these studies, 67.6% are published articles and manuscripts, 24% are unpublished dissertations, and 43.2% of the studies took place in the public sector. The results suggest that the level of employees' perceived OD was slightly above the medium level (mean: 3.30 ± 81), and the scores of the private-sector employees are higher than those of the public employees. Further, empirical associations between OD and 21 different outcome variables are reported and discussed. To varying extents, significant positive correlations were found between the level of employees' perceived OD and positive organizational variables, such as organizational citizenship behavior, organizational commitment, psychological capital, and job satisfaction. On the other hand, negative significant relationships occurred between OD and negatively evaluated organizational variables, such as job stress and organizational depression. The results of this study support the importance of organizational democracy as a management approach.
The Effectiveness of Plaza Dancing on Psychological Well-Being and Ill-Being: A Systematic Review and Meta-Analysis
Authors: Bai Zhenggang , Li Yixuan , Yang Yufan , Xie Chengdan , Zhu Zhengyun , Xu Yan , Bai Ruhai
Published: April 2022

Evidence suggests that participation in plaza dancing may affect mental health. This study for the first time quantified the relationships between plaza dancing and psychological well-being and ill-being. We systematically searched PubMed, Web of Science, CNKI, Wanfang, and VIP to identify relevant studies published from the databases since their inception to July 25, 2021. The standardized mean differences (SMDs) of pre-to-post intervention data were calculated in the meta-analysis. Subgroup and meta-regression analyses were performed to test the potential moderating effects of age, outcome classification, measurement instruments, district, publication year, total sample size, and the duration, frequency, and length of the square dance intervention. A total of 25 original articles met all the eligibility criteria and were included in the review, and 17 studies were included in the meta-analysis. The meta-analysis revealed that plaza dancing improved psychological well-being (pooled SMD = 0.76; 95% CI: 0.58, 0.95; I2 = 86.9%) and reduced psychological ill-being (pooled SMD = −0.84; 95% CI: −1.00, −0.68; I2 = 64.8%). The study participants’ age and district did not seem to affect the effectiveness of the plaza dancing intervention. The duration and frequency of plaza dancing affected the association between square dance and psychological well-being (duration, β = −0.044; 95% CI: −0.085, −0.004; frequency, β = 0.122; 95% CI: 0.024, 0.221) and psychological ill-being (duration, β = −0.029; 95% CI: −0.040, −0.018; frequency, β = 0.154; 95% CI: 0.030, 0.278). Plaza dancing has a significant positive effect on psychological well-being and psychological ill-being, and the effects are moderated by intervention modality. Generalizing plaza dancing interventions to promote psychological well-being and prevent or treat psychological ill-being is needed.

Systematic Review Registration

[www.crd.york.ac.uk/prospero], identifier [CRD42021272016].
Mental Health and Loneliness in University Students During the COVID-19 Pandemic in Germany: A Longitudinal Study
Authors: Weber Maxi , Schulze Lars , Bolzenkötter Teresa , Niemeyer Helen , Renneberg Babette
Published: April 2022

The COVID-19 pandemic and its preventive measures had adverse consequences for mental health. However, knowledge of mental health trajectories across the pandemic is limited. This study investigated the mental health levels and changes among university students during the pandemic and lockdown in Germany, as well as their associated factors. We surveyed students' mental health (N = 363, 68% female) with the patient health questionnaire (PHQ-8) and the generalized anxiety disorder scale (GAD-7) during the first easing phase (July 2020; time 1) and the second lockdown (November 2020; time 2). Cut-off scores from the GAD-7 and PHQ-8 were used to determine clinically relevant symptoms and to define trajectory groups. Sociodemographic and pandemic-related data were assessed (e.g., coping with academic life, social contacts) as well as loneliness, stress, repetitive negative thinking, quality of life, and perceived social support. Paired t-test, multiple regression, and repeated-measures ANOVA were applied. Means and prevalence rates for symptoms of depression (38.8%) and anxiety (25.6%) did not differ between time 1 and time 2, and most students were asymptomatic on the PHQ-8 (44.4%) and the GAD-7 (56.3%) across the pandemic. Feelings of loneliness significantly increased from time 1 to time 2, d = −0.30, [−0.47, −0.13], with higher symptom levels in symptomatic groups at time 2 and greater increases in the asymptomatic groups. Levels of stress, repetitive negative thinking, quality of life, and social support did not differ during the pandemic. At time 1, loneliness and repetitive negative thinking were associated with anxiety and depressive symptoms. Anxiety and depressive symptoms were prevalent among students, and increased levels of loneliness during the pandemic were associated with elevated symptoms and differing trajectories. Further research using representative and larger samples should determine the long-term impact of the pandemic on mental health and loneliness to identify vulnerable students and offer adequate support.
Correlation Analysis of Serum Vitamin D Levels and Postoperative Cognitive Disorder in Elderly Patients With Gastrointestinal Tumor
Authors: Zhang Jialei , Zhang Xiaoling , Yang Yongyan , Zhao Jun , Yu Yonghao
Published: April 2022

Purpose

Vitamin D prevents hypocalcaemia, osteoporosis, and infections, among other problems, and is involved in the prevention and treatment of cardiovascular and neurological diseases. Recently, vitamin D was shown to improve cognitive dysfunction caused by Alzheimer's disease and vascular dementia. This study aims to explore the correlation between preoperative serum vitamin D and postoperative cognitive disorder (POCD) occurrence in elderly patients with gastrointestinal tumors to guide perioperative medication use and promote early patient recovery.

Methods

This study recruited 238 elderly patients (65 ≤ age ≤ 85) who underwent gastrointestinal tumor surgery; 117 cases were enrolled, and 55 controls of the same age and education level as the cases were included. Blood samples were taken preoperatively and at 7, 15, 30, and 90 days postoperatively, and plasma vitamin D (25OH-D3) and glutathione (GSH) was measured. Different from the previous diagnosis of POCD was obtained by telephone interview through Cognitive Status Modified Telephone Interview (TICS-m), mainly for memory impairment, a series of neuropsychological tests was used to evaluate cognitive function, Picture Recollect Test, Stroop Color-word Test, and Digit Symbol Substitution Test were used to comprehensively evaluate the three domains of cognitive function of patients, namely memory, attention and information processing ability. All neuropsychiatric assessments were performed at the bedside and completed face-to-face by the assessment staff and the patient.

Results

A total of 65.8% (77/117) of elderly patients undergoing gastrointestinal tumor surgery had preoperative vitamin D deficiency (serum 25OH-D concentration < 12 ng/ml), of whom 46.7% (36/77, 7 days after surgery), 31.2% (24/77, 15 days after surgery), 15.6% (12/77, 30 days after surgery), and 9% (7/77, 90 days after surgery) of patients developed POCD; 7.5% (3/40) of patients without vitamin D deficiency developed PNDs, which was detected only on the 7th day after surgery.

Conclusions

Vitamin D deficiency can increase neurocognitive disorder risk in elderly patients during the perioperative period, possibly because low vitamin D levels cannot effectively inhibit the postoperative oxidative stress increase.

Trial Registration

This experiment was approved and registered by the China Clinical Trial Registration Center, registration number ChiCTR2100046900 (30/05/2021).
A Comprehensive Evaluation of Health-Related Life Quality Assessment Through Head and Neck, Prostate, Breast, Lung, and Skin Cancer in Adults
Authors: Jalili Shirin , Ghasemi Shayan Ramin
Published: April 2022

Health assessment data assists the well-being and patient care teams' process in drawing up a care and assistance plan and comprehending the requirements of the patient. Comprehensive and precise data about the Quality of Life of cancer patients play a significant part in the development and organization of cancer patient care. Quality of Life has been used to mean a variety of various things, such as health situation, physical function, symptoms, psychosocial modification, well-being, enjoyment of life, and happiness. Chronic diseases such as cancer are among the disorders that severely affect people's health and consequently their Quality of Life. Cancer patients experience a range of symptoms, including pain and various physical and mental conditions that negatively affect their Quality of Life. In this article, we examined cancer and the impact that this disease can have on the Quality of Life of cancer patients. The cancers examined in this article include head and neck, prostate, breast, lung, and skin cancers. We also discussed health assessment and the importance and purpose of studying patients' Quality of Life, especially cancer patients. The various signs and symptoms of the disease that affect the Quality of Life of patients were also reviewed.
Inequity in Health Services Utilization in Economically Underdeveloped Regions of Northeast China
Authors: Zhang Xin , Ning Ning , Zhou Hongguo , Shan Linghan , Hao Yanhua , Jiao Mingli , Liang Libo , Kang Zheng , Li Ye , Liu Huan , Liu Baohua , Wang Kexin , Ruzieva Adelina , Gao Lijun , Wu Qunhong
Published: April 2022

Background

The Chinese health system has long been committed to eliminating inequalities in health services utilization. However, few studies have analyzed or measured these inequalities in economically underdeveloped regions in China.

Methods

A total of 6,627 respondents from 3,000 households in Heilongjiang Province were extracted from the Sixth National Health Services Survey. We measured horizontal inequity in both 2-week outpatient rate and annual inpatient rate, and then identified the factors contributing to inequality.

Results

The horizontal inequity indices of the 2-week outpatient and annual impatient rates in Heilongjiang Province were 0.0586 and 0.1276, respectively. Household income, health status, place of residence, basic medical insurance, and commercial health insurance were found to be the main factors affecting inequality in health services utilization. The contributions of household income to these two indices were 184.03 and 253.47%, respectively. Health status factors, including suffering from chronic disease, limitations in daily activities, and poor self-rated health, played positive roles in reducing inequality in these two indices. The contributions of place of residence to these two indices were 27.21 and −28.45%, respectively. Urban Employee Basic Medical Insurance made a pro-rich contribution to these two indices: 56.25 and 81.48%, respectively. Urban and Rural Resident Basic Medical Insurance, Urban Resident Basic Medical Insurance, New Rural Cooperative Medical Scheme, and other basic medical insurance made a pro-poor contribution to these two indices: −73.51 and −54.87%, respectively. Commercial health insurance made a pro-rich contribution to these two indices: 20.79 and 7.40%, respectively. Meanwhile, critical illness insurance made a slightly pro-poor contribution to these two indices: −4.60 and −0.90%, respectively.

Conclusions

The findings showed that the “equal treatment in equal need” principle was not met in the health services utilization context in Heilongjiang Province. To address this issue, the government could make policy changes to protect low-income populations from underused health services, and work to improve basic medical insurance, critical illness insurance, and social security systems.
Decision Support System for Predicting Survivability of Hepatitis Patients
Authors: Albogamy Fahad R. , Asghar Junaid , Subhan Fazli , Asghar Muhammad Zubair , Al-Rakhami Mabrook S. , Khan Aurangzeb , Nasir Haidawati Mohamad , Rahmat Mohd Khairil , Alam Muhammad Mansoor , Lajis Adidah , Su'ud Mazliham Mohd
Published: April 2022

Background and Objective

Viral hepatitis is a major public health concern on a global scale. It predominantly affects the world's least developed countries. The most endemic regions are resource constrained, with a low human development index. Chronic hepatitis can lead to cirrhosis, liver failure, cancer and eventually death. Early diagnosis and treatment of hepatitis infection can help to reduce disease burden and transmission to those at risk of infection or reinfection. Screening is critical for meeting the WHO's 2030 targets. Consequently, automated systems for the reliable prediction of hepatitis illness. When applied to the prediction of hepatitis using imbalanced datasets from testing, machine learning (ML) classifiers and known methodologies for encoding categorical data have demonstrated a wide range of unexpected results. Early research also made use of an artificial neural network to identify features without first gaining a thorough understanding of the sequence data.

Methods

To help in accurate binary classification of diagnosis (survivability or mortality) in patients with severe hepatitis, this paper suggests a deep learning-based decision support system (DSS) that makes use of bidirectional long/short-term memory (BiLSTM). Balanced data was utilized to predict hepatitis using the BiLSTM model.

Results

In contrast to previous investigations, the trial results of this suggested model were encouraging: 95.08% accuracy, 94% precision, 93% recall, and a 93% F1-score.

Conclusions

In the field of hepatitis detection, the use of a BiLSTM model for classification is better than current methods by a significant margin in terms of improved accuracy.
Adult Vaccination in the United Arab Emirates—A Physicians' Knowledge and Knowledge Sources Study
Authors: Barqawi Hiba J. , Samara Kamel A. , Hassan Mahmoud S. , Amawi Firas B.
Published: April 2022

Background

A lack of knowledge on adult vaccination has been documented among physicians. They play a critical role in promoting adult vaccines. This study aimed to review the status of adult vaccination in the United Arab Emirates (UAE) and evaluate physicians' knowledge and knowledge sources regarding adult vaccines.

Methods

Local, regional, and global adult vaccination guidelines were reviewed. A 40-item questionnaire was used to collect data from physicians from June to October 2020, using convenience and snowball sampling. Knowledge score was calculated, and predictors identified using Mann–Whitney U and Kruskal–Wallis H-tests. Ordinary Least Squares regression was used for Multivariate Analysis.

Results

A total of 500 responses were included. A quarter were internists, and another quarter were family physicians. Fifty-seven percent were medical interns and residents. Both perceived and actual knowledge of adult vaccination were low. Bivariate analysis showed knowledge depending on department, level of training, workplace, and perceived knowledge. All remained significant after multivariable regression except workplace. International and local guidelines were the most common knowledge sources. Forty-two percent were unable to access the local guidelines.

Conclusions

Physicians' knowledge was poor and local guidelines were not clear or easily accessible. Participants were highly receptive to guidance and practice with adult vaccines.
Plants Can “Speak” to Each Other
Authors: Hanano Abdulsami , Murphy Colette , Murphy Denis J.
Published: April 2022

When we think about plants, we do not normally imagine them “speaking” to each other. But they do communicate—in many different ways. More than a century ago, the eminent biologist Charles Darwin suggested that plants have a brain-like structure at their root tips! In this case, Darwin’s root-brain hypothesis was wrong, but more modern research shows that plants can communicate. They speak with other plants as well as with animals and even people. They do this primarily using chemicals and sound.
How Does Your Body Deal With Fast-Food Meals?
Authors: Poupin Nathalie , Polakof Sergio
Published: April 2022

Eating a less-than-healthy diet can increase the chances of becoming obese, which usually happens when body organs have difficulties using or storing the excess fat and sugar that we eat. To better understand how what we eat affects the functioning of our organs, we measured substances called metabolites, which come from food, in the blood of obese minipigs. We then used computers to figure out what happens to these metabolites inside the liver following normal meals or a fast-food meal. We found that the liver fights against unhealthy food by finding ways to use or remove the excess fat and sugar. However, some of the paths normally followed by healthy food might become blocked when we eat too much unhealthy food. Our work showed that a better understanding of how the liver processes the metabolites from unhealthy diets could help people suffering from the effects of obesity.
Surgical Management of Small Bowel Crohn's Disease
Authors: Chandrasinghe Pramodh
Published: April 2022

Crohn's disease in the small bowel could present itself as an inflammatory stricture, a fibrotic stricture as penetrating disease or a combination of both. It is pertinent to differentiate the disease process as well as its extent to effectively manage the disease. Currently, a combination of medical and surgical therapies forms part of the treatment plan while the debate of which therapy is better continues. In managing the strictures, identification of the disease process through imaging plays a pivotal role as inflammatory strictures respond to anti-tumor necrosis factor (TNF) and biological agents, while fibrotic strictures require endoscopic or surgical intervention. Recent evidence suggests a larger role for surgical excision, particularly in ileocolic disease, while achieving a balance between disease clearance and bowel preservation. Several adaptations to the surgical technique, such as wide mesenteric excision, side to side or Kono-S anastomosis, and long-term metronidazole therapy, are being undertaken even though their absolute benefit is yet to be determined. Penetrating disease requires a broader multidisciplinary approach with a particular focus on nutrition, skincare, and intestinal failure management. The current guidance directs toward early surgical intervention for penetrating disease when feasible. Accurate preoperative imaging, medical management of active diseases, and surgical decision-making based on experience and evidence play a key role in success.
Mixed Reality Technology in Total Knee Arthroplasty: An Updated Review With a Preliminary Case Report
Authors: Su Shilong , Lei Pengfei , Wang Chenggong , Gao Fawei , Zhong Da , Hu Yihe
Published: April 2022

Background

Augmented reality and mixed reality have been used to help surgeons perform complex surgeries. With the development of technology, mixed reality (MR) technology has been used to improve the success rate of complex hip arthroplasty due to its unique advantages. At present, there are few reports on the application of MR technology in total knee arthroplasty. We presented a case of total knee arthroplasty with the help of mixed reality technology.

Case Presentation

We presented a case of a 71-year-old woman who was diagnosed with bilateral knee osteoarthritis with varus deformity, especially on the right side. After admission, the right total knee arthroplasty was performed with the assistance of MR technology. Before the operation, the three-dimensional virtual model of the knee joint of the patient was reconstructed for condition analysis, operation plan formulation, and operation simulation. During the operation, the three-dimensional virtual images of the femur and tibia coincided with the real body of the patient, showing the osteotomy plane designed before the operation, which can accurately guide the completion of osteotomy and prosthesis implantation.

Conclusions

As far as we know, this is the first report on total knee arthroplasty under the guidance of mixed reality technology.
Involvement of MiRNA-211-5p and Arhgap11a Interaction During Osteogenic Differentiation of MC3T3-E1 Cells
Authors: Ju Wenwen , Zhang Guangfeng , Zhang Xu , Wang Jingting , Wu Tong , Li Huafeng
Published: April 2022

Objective

MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells.

Methods

An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors.

Results

We identified 715 upregulated DEGs and 603 downregulated DEGs between MC3T3-E1 cells with and without osteoblast induction by analyzing the raw data of the GSE46400 dataset. There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. The RT-qPCR also determined declined expressions of miR-204-5p and miR-24-3p concomitant with elevated expressions of miR-211-5p, miR-3470b, miR-466b-3p, and miR-466c-3p in the MC3T3-E1 cells, with osteoblast induction compared with undifferentiated MC3T3-E1 cells. Dual-luciferase reporter gene assays demonstrated Arhgap11a as the target of miR-211-5p. MiR-211-5p upregulation by its mimic increased Arhgap11a expression in MC3T3-E1 cells.

Conclusion

Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair.
The Transumbilical Laparoendoscopic Single-Site Extraperitoneal Approach for Pelvic and Para-Aortic Lymphadenectomy: A Technique Note and Feasibility Study
Authors: Peng Shiyi , Zheng Ying , Yang Fan , Wang Kana , Chen Sijing , Wang Yawen
Published: April 2022

Background

Nowadays, lymphadenectomy could be performed by the transperitoneal or extraperitoneal approach. Nevertheless, each approach has its own advantages and disadvantages. Under these circumstances, we developed a transumbilical laparoendoscopic single-site (TU-LESS) extraperitoneal approach for lymphadenectomy. In this research, the primary goal is to demonstrate the feasibility of the novel approach in systematic lymphadenectomy and present the surgical process step-by-step.

Methods

Between May 2020 and June 2021, patients who had the indications of systematic lymphadenectomy underwent lymphadenectomy via the TU-LESS extraperitoneal approach. This new approach was described in detail, and the clinical characteristics and surgical outcomes were collected and analyzed.

Results

Eight patients with gynecological carcinoma were included in the research, including four with high-risk endometrial cancer and four with early-stage ovarian cancer. The TU-LESS extraperitoneal approach for pelvic and para-aortic lymphadenectomy was successfully performed in all patients without conversion. In all, a median of 26.5 pelvic lymph nodes (range 18–35) and 18.0 para-aortic lymph nodes (range 7–43) were retrieved. There was a median of 166.5 min of surgical time (range 123–205). Patients had speedy recoveries without complications. All patients had positive pain responses after surgery, as well as satisfactory cosmetic and body image outcomes.

Conclusion

Our initial experience showed that it is feasible to perform systematic lymphadenectomy with the TU-LESS extraperitoneal approach. And this new approach may provide a new measure or a beneficial supplement for lymphadenectomy in gynecologic cancer.
Clinical, Diagnostic, and Imaging Findings in Three Juvenile Dogs With Paraspinal Hyperesthesia or Myelopathy as a Consequence of Hemophilia A: A Case Report
Authors: Fowler Kayla M. , Bolton Timothy A. , Rossmeisl John H. , Arendse Avril U. , Vernau Karen M. , Li Ronald H. L. , Parker Rell L.
Published: April 2022

Three juvenile dogs presented with an acute onset of paraspinal hyperesthesia and/or neurologic deficits. These dogs underwent anesthesia for MRI and additional diagnostics. The thoracolumbar MRI in Dog 1 revealed an accumulation of T2-weighted (T2W) hyperintense, T1-weighted (T1W) iso- to hyperintense, contrast enhancing extradural material. The differential diagnoses were meningitis with secondary hemorrhage or empyema or late subacute hemorrhage. The initial cervical MRI in Dog 2 revealed T1W meningeal contrast enhancement suspected to be secondary to meningitis. A repeat MRI following neurologic decline after CSF sampling revealed a large area of T2W and T1W hyperintensity between fascial planes of the cervical musculature as well as T2W iso- to hyperintense and T1W iso- to hypointense extradural material at the level of C1 consistent with hemorrhage. The cervical MRI in Dog 3 revealed T2W hyperintense and T1W iso- to hypointense extradural compressive material consistent with hemorrhage. Dogs 1 and 2 underwent CSF sampling and developed complications, including subcutaneous hematoma and vertebral canal hemorrhage. Dog 3 underwent surgical decompression, which revealed a compressive extradural hematoma. In each case, a hemophilia panel including factor VIII concentration confirmed the diagnosis of hemophilia A. Dog 1 had a resolution of clinical signs for ~5 months before being euthanized from gastrointestinal hemorrhage. Dog 2 was euthanized due to neurologic decompensation following CSF sampling. Dog 3 did well for 2 weeks after surgery but was then lost to follow-up. This case series provides information on clinical signs, MRI findings, and outcome in 3 juvenile dogs with hemophilia A that developed neurologic deficits or paraspinal hyperesthesia secondary to spontaneous or iatrogenic vertebral canal hemorrhage. Hemophilia A should be considered as a differential in any young dog presenting with an acute onset of hyperesthesia with or without neurologic deficits. This diagnosis should be prioritized in young male dogs that have other evidence of hemorrhage on physical exam.
Preliminary Analysis of Compression System Integrated Heat Management Concepts Using LH2-Based Parametric Gas Turbine Model
Authors: Abedi Hamidreza , Xisto Carlos , Jonsson Isak , Grönstedt Tomas , Rolt Andrew
Published: April 2022

The investigation of the various heat management concepts using LH2 requires the development of a modeling environment coupling the cryogenic hydrogen fuel system with turbofan performance. This paper presents a numerical framework to model hydrogen-fueled gas turbine engines with a dedicated heat-management system, complemented by an introductory analysis of the impact of using LH2 to precool and intercool in the compression system. The propulsion installations comprise Brayton cycle-based turbofans and first assessments are made on how to use the hydrogen as a heat sink integrated into the compression system. Conceptual tubular compact heat exchanger designs are explored to either precool or intercool the compression system and preheat the fuel to improve the installed performance of the propulsion cycles. The precooler and the intercooler show up to 0.3% improved specific fuel consumption for heat exchanger effectiveness in the range 0.5–0.6, but higher effectiveness designs incur disproportionately higher pressure losses that cancel-out the benefits.
Numerical Evaluation of Riblet Drag Reduction on a MALE UAV
Authors: Bliamis Chris , Vlahostergios Zinon , Misirlis Dimitrios , Yakinthos Kyros
Published: April 2022

Flow control methods for aerodynamic drag reduction have been a field of interest to aircraft designers, who seek to minimize fuel consumption and increase the aircraft’s aerodynamic performance. Various flow control techniques, applied to aeronautical applications ranging from large airliners to small hand-launched unmanned aerial vehicles (UAVs), have been conceptualized, designed and tested in the past. Among others, the concept of riblets, inspired by the shark’s skin morphology, has been proposed and evaluated for airliners. In this work, the implementation of riblets on a medium-altitude long-endurance UAV (MALE) is investigated. The riblets can offer drag reduction due to the decrease in total skin friction, by altering the boundary layer characteristics in the near-wall region. The riblets are implemented on specific locations on the UAV (main wing, fuselage and empennage) and appropriately selected, on which the boundary layer becomes transitional from the laminar to the turbulent flow regime. For this reason, computational fluid dynamics modelling is performed by solving the Reynolds-averaged Navier–Stokes equations, incorporating the k-ω SST eddy viscosity turbulence model. The effect of the riblets in the near-wall region is modelled with the use of an appropriate wall boundary condition for the specific turbulence dissipation rate transport equation. It is shown that a drag reduction benefit, for both the loiter and the cruise flight segments of the UAV mission, can be obtained, and this is clearly presented by the drag polar diagrams of the air vehicle. Finally, the potential benefit to flight performance in terms of endurance and payload weight increase is also evaluated.
The Economic Burden of Cancer in Canada from a Societal Perspective
Authors: Garaszczuk Roxanne , Yong Jean H. E. , Sun Zhuolu , de Oliveira Claire
Published: April 2022

Cancer patients and their families experience considerable financial hardship; however, the current published literature on the economic burden of cancer at the population level has typically focused on the costs from the health system’s perspective. This study aims to estimate the economic burden of cancer in Canada from a societal perspective. The analysis was conducted using the OncoSim-All Cancers model, a Canadian cancer microsimulation model. OncoSim simulates cancer incidence and deaths using incidence and mortality data from the Canadian Cancer Registry and demography projections from Statistics Canada. Using a phase-based costing framework, we estimated the economic burden of cancer in Canada in 2021 by incorporating published direct health system costs and patients’ and families’ costs (out-of-pocket costs, time costs, indirect costs). From a societal perspective, cancer-related costs were CAD 26.2 billion in Canada in 2021; 30% of costs were borne by patients and their families. The economic burden was the highest in the first year after cancer was diagnosed (i.e., initial care). During this time, patients and families’ costs amounted to almost CAD 4.8 billion in 2021. This study provides a comprehensive estimate of the economic burden of cancer, which could inform cost–benefit analyses of proposed cancer prevention interventions.
Quality Analysis of Commercially Available Wood Pellets and Correlations between Pellets Characteristics
Authors: Kamperidou Vasiliki
Published: April 2022

In this study, the quality of wood fuel pellets, commercially available in the Greek market, was evaluated using the ISO 17225 Standard Requirement Thresholds as a benchmark. The examined quality criteria were calorific value, ash content, mechanical durability, bulk density and moisture content. The reliability of the information on packages was evaluated. Meanwhile, the potential correlation between the most crucial qualitative properties of pellets was investigated to elucidate their potential contribution to pellets’ quality estimation. Most of the pellet brands showed low moisture and ash content and acceptable calorific values for residential use—although the quality of half of the pellet brands was found to be lower than that reported, mainly with regard to the mechanical durability and bulk density. The application of two different collection periods highlighted a stability in quality over time. Higher calorific value seemed to be provided by pellets which had low ash and low moisture content and were mechanically durable. As regards the specific samples, a dark colour and high roughness were correlated with lower fuel quality (impact of 56.2% and 43.6%, respectively).
Dynamics and Control of an Energy-Efficient, Power-Regenerative, Hydrostatic Wind Turbine Dynamometer
Authors: Mohanty Biswaranjan , Stelson Kim A.
Published: April 2022

Dynamometers are used to evaluate the real-world performances of drivetrains in various loading conditions. Due to its superior power density, high bandwidth, and design flexibility, a hydrostatic power-regenerative dynamometer is an ideal candidate for hydrostatic wind turbine transmission testing. A dynamometer can emulate the wind turbine rotor dynamics and allow the investigation of the performance of a unique hydrostatic drivetrain without actually building the physical system. The proposed dynamometer is an energy-efficient system with counter-intuitive control challenges. This paper presents the dynamics, control synthesis, and experimental validation of a power-regenerative hydrostatic dynamometer. A fourth-order non-linear model with three inputs was formulated for the dynamometer. The strength of input–output couplings was identified, and two different decoupling controllers were designed and implemented. During wind turbine testing, the synchronous generator turns at a constant speed and the system model is linear. A steady-state decoupling controller was developed for independent control of the drive and transmission. The implemented decoupling controller demonstrated a negligible change in rotor speed for a 40 bar step increase in pressure, but a 20 bar pressure spike for a 4 rpm step change in rotor speed. However, during starting and stopping, the synchronous generator speed is not constant, and the system model is nonlinear. Therefore, a steady-state decoupling controller will not work. Thus, a decentralized controller with feed-forward control and gain scheduling was designed and implemented. A reference command was designed to avoid cavitation, pressure spikes, and power flow reversal during start-up. The experimental results show precise tracking in steady-state and transient operations. The decentralized controller demonstrated a negligible change in rotor speed for a 40 bar step increase in pressure but a 100 bar pressure spike for a 4 rpm step increase in rotor speed. The pressure spike was reduced by 80 bar with the implementation of feed-forward gain. The proposed electro-hydro-mechanical system requires less power and has the potential to reduce energy expenditure by 50%.
An Improved Approach to Calculate Eddy Current Loss in Soft Magnetic Materials Based on Measured Hysteresis Loops
Authors: Jabłoński Paweł , Najgebauer Mariusz , Bereźnicki Michał
Published: April 2022

The paper presents a study of the influence of magnetic hysteresis on macroscopic eddy current losses. Usually, this loss is calculated within many simplifying assumptions, of which the most striking is not only neglecting the hysteresis, but also the nonlinearity of the material. To check how such simplifications affect the results, the loss was calculated taking into account the hysteresis phenomenon for several materials. For this purpose, an algorithm for solving Maxwell’s equations with magnetic characteristics given as a family of measured hysteresis loops was constructed. A new method for conversion between H and B fields using the measured hysteresis loops was also proposed. The results of calculations for three materials show that the eddy current loss calculated with magnetic hysteresis taken into account are smaller than those calculated via the classical formula obtained for linear materials. For the tested materials, the differences reach up to around 30%, depending on frequency, magnetic flux density and thickness of electrical sheet. This may result, among others, in erroneous determination of the excess loss in Bertotti’s approach.
Realistic Simulation of Sensor/Actuator Faults for a Dependability Evaluation of Demand-Controlled Ventilation and Heating Systems
Authors: Kiamanesh Bahareh , Behravan Ali , Obermaisser Roman
Published: April 2022

In the development of fault-tolerant systems, simulation is a common technique used to obtain insights into performance and dependability because it saves time and avoids the risks of testing the behavior of real-world systems in the presence of faults. Fault injection in a simulation offers a high controllability and observability, and thus is ideal for an early dependability analysis and fault-tolerance evaluation. Heating, ventilation, and air conditioning (HVAC) systems in critical infrastructures, such as airports and hospitals, are safety-relevant systems, which not only determine energy consumption, system efficiency, and occupancy comfort but also play an essential role in emergency scenarios (e.g., fires, biological hazards). Hence, fault injection serves as a practical and essential solution to assess dependability in different fault scenarios of HVAC systems. Hence, in this paper, we present a simulation-based fault injection framework with a combination of two techniques, simulator command and simulation code modification, which are applied to fault injector blocks as saboteurs and an automated fault injector algorithm to automatically activate fault cases with certain fault attributes. The proposed fault injection framework supports a comprehensive range of faults and various fault attributes, including fault persistence, fault type, fault location, fault duration, and fault interarrival time. This framework considers noise in a demand-controlled ventilation (DCV) and heating system as a type of HVAC system since it has been demonstrated that any fault injection scenario is accompanied by some impacts on energy consumption, occupancy comfort, and a fire risk. It also supports the reproducibility for a set of specific fault scenarios or random fault injection scenarios. The system model was implemented and simulated in Matlab/Simulink, and fault injector blocks were developed by Stateflow diagrams. An experimental evaluation serves as the assessment of the presented fault injection framework with a defined example of fault scenarios. The results of the evaluation show the correctness, system behavior, accuracy, and other parameters of the system, such as the heater energy consumption and heater duty cycle of the fault injection framework in the presence of different fault cases. In conclusion, the present paper provides a novel simulation-based fault injection framework, which combines simulator command techniques and simulation code modifications for a realistic and automatic fault injection with comprehensive coverage of various fault types and a consideration of noise and uncertainty, allowing for reproducibility of the results. The outputs achieved from the fault injection framework can be applied to fault-tolerant studies in other application domains.