bluuebunny/crossref_metadata_2025 huggingface dataset:
iGovernment
Authors: Prins Corien , Broeders Dennis , Griffioen Henk , Keizer Anne-Greet , Keymolen Esther
Published: January 2021

The authors of this incisive study explore the problems of the ongoing digitization of government, such as the creeping loss of data quality, and how citizens and officials must respond to these complications in the coming years. The iGovernment is running full speed on information networks and digitization, but it is also seriously out of step with existing bureaucracies. iGovernment offers an accurate picture of how the digital technologies are shaping modern governments, and also a powerful corrective for the dissonance between technology and organizational management.

'This book will be a valuable resource for researchers and scholars seeking to understand the possibilities, dilemmas, and challenges of bringing the Internet and related technologies to center stage in government and public services' Helen Margetts, University of Oxford
Signature Style of Frans Hals
Authors: Atkins Christopher D. M.
Published: January 2021

This richly illustrated study is the first consider the manifold functions and meanings of Hals's distinctive handling of paint. Atkins explores the uniqueness of Hals's approach to painting and the relationship of his manner to seventeenth-century aesthetics. He also investigates the economic motivations and advantages of his methods, the operation of the style as a personal and workshop brand, and the apparent modernity of the artist's style. The book seeks to understand the multiple levels on which Hals's consciously cultivated manner of painting operated for himself, his pupils and assistants, his clients, and succeeding generations of viewers. As a result, the book offers a wholly new understanding of one of the leading artists of the Dutch Golden Age, and one of the most formative painters in the history of art in the Western tradition. It also provides a much needed interrogation of the interrelationships of subjectivity, style, authorship, methods of artistic and commercial production, economic consumption, and art theory in early modernity.
Accounting for Skewed or One-Sided Measurement Error in the Dependent Variable
Authors: Millimet Daniel L. , Parmeter Christopher F.
Published: January 2021

While classical measurement error in the dependent variable in a linear regression framework results only in a loss of precision, nonclassical measurement error can lead to estimates, which are biased and inference which lacks power. Here, we consider a particular type of nonclassical measurement error: skewed errors. Unfortunately, skewed measurement error is likely to be a relatively common feature of many outcomes of interest in political science research. This study highlights the bias that can result even from relatively “small” amounts of skewed measurement error, particularly, if the measurement error is heteroskedastic. We also assess potential solutions to this problem, focusing on the stochastic frontier model and Nonlinear Least Squares. Simulations and three replications highlight the importance of thinking carefully about skewed measurement error as well as appropriate solutions.
DIVISIBILITY OF CERTAIN SINGULAR OVERPARTITIONS BY POWERS OF
AND
Authors: SINGH AJIT , BARMAN RUPAM
Published: January 2021

Andrews introduced the partition function

$\overline {C}_{k, i}(n)$
, called the singular overpartition function, which counts the number of overpartitions of n in which no part is divisible by k and only parts

$\equiv \pm i\pmod {k}$
may be overlined. We prove that

$\overline {C}_{6, 2}(n)$
is almost always divisible by

$2^k$
for any positive integer k. We also prove that

$\overline {C}_{6, 2}(n)$
and

$\overline {C}_{12, 4}(n)$
are almost always divisible by

$3^k$
. Using a result of Ono and Taguchi on nilpotency of Hecke operators, we find infinite families of congruences modulo arbitrary powers of

$2$
satisfied by

$\overline {C}_{6, 2}(n)$
.
The longitudinal associations between trajectory of and quantity of alcohol consumption and subsequent changes in blood pressure levels among non-hypertensive adults
Authors: Jung Sukyoung , Kim Mi Kyung , Shin Jinho , Choi Bo Youl , Lee Young-Hoon , Shin Dong Hoon , Shin Min-Ho
Published: January 2021

Given the dynamic characteristic of an individual’s drinking behaviours, comprehensive consideration of alcohol consumption variation using repeated measures may improve insight into the nature of its association with blood pressure (BP) change. We examined the association between longitudinal alcohol consumption (trajectory and quantity) and changes in BP and pulse pressure (PP) among Korean aged ≥ 40 years living in rural areas. Totally, 1682 hypertension-free participants who completed all three health examinations (median, 5·3 years) were included. All three visits were used to determine the cumulative trajectory of and quantity of alcohol consumption and the latest two visits and the last visit were used for the recent trajectory and the most recent quantity of alcohol consumption, respectively. Changes in BP and PP from the baseline to the third visit were used as outcome. In men, ≥30 ml/d cumulative average alcohol consumption was associated with the greatest increase in systolic BP (SBP) in both baseline outcome-unadjusted (2·9 mmHg, P = 0·032) and -adjusted models (3·6 mmHg, P = 0·001), and the given association for the most recent alcohol consumption was observed in the baseline outcome-adjusted model (3·9 mmHg, P = 0·003). For PP, similar associations were observed only in the baseline outcome-adjusted model. No meaningful associations in diastolic BP in men and any BP or PP in women existed. The quantity of alcohol consumption rather than the trajectory may be significantly related to raised SBP, and a possible short-term influence of the most recent alcohol consumption may exist when baseline SBP is adjusted in men.
Lex Generalis Derogat Legi Speciali: IHL in Human Rights Regulation of Military Courts Operating in Situations of Armed Conflict
Authors: Herzberg Anne
Published: January 2021

The operation of military courts is clearly allowed for and, in some cases, mandated by international humanitarian law (IHL). Nevertheless, the use of military courts has been one of the most controversial and hotly debated areas of human rights. Despite the ostensibly exclusive military domain, many human rights bodies have registered significant scepticism towards this type of justice. Consequently, they have sought actively to regulate this ‘IHL space’ with scant attention to the requirements of IHL itself. The article examines comments, case law, draft rules and other measures taken by two human rights frameworks: the United Nations Human Rights Council and the African Commission on Human and People's Rights. It will analyse how, since 2000, these bodies have approached the issue of IHL when assessing the legitimacy and operation of military courts. For instance, do they consider IHL as a source of law guiding their efforts and rely on IHL instruments? How do they resolve conflicts between IHL and international human rights law? Additionally, the article will consider the validity, legality and effectiveness of these efforts. It concludes that, in reviewing military courts, there exists significant neglect of IHL in human rights frameworks. Through overlooking IHL or relegating it to a sub-specialty of international human rights law, these bodies not only ignore applicable law, they deprive themselves of the wealth of expertise found in commentary, debate, jurisprudence and practice in the IHL sphere. Instead, integrating IHL analysis and theory and affording it its appropriate respect within relevant human rights discussions will allow for greater legal and policy coherence, and human rights bodies will be better placed to fulfil their mandates.
Boxes, extended boxes and sets of positive upper density in the Euclidean space
Authors: DURCIK POLONA , KOVAČ VJEKOSLAV
Published: January 2021

We prove that sets with positive upper Banach density in sufficiently large dimensions contain congruent copies of all sufficiently large dilates of three specific higher-dimensional patterns. These patterns are: 2n vertices of a fixed n-dimensional rectangular box, the same vertices extended with n points completing three-term arithmetic progressions, and the same vertices extended with n points completing three-point corners. Our results provide common generalizations of several Euclidean density theorems from the literature.
Francesco De Pinedo and Ernesto Campanelli's record-breaking flight to Australia – perception, recognition and legacy: an account in the Australian Press
Authors: Cauli Alberto
Published: January 2021

The year 2020 marked the 95th anniversary of Francesco De Pinedo and Ernesto Campanelli's record-breaking flight of 55,000 km, from Italy to Australia, Japan and back, in a seaplane namedGennariello. Their achievement was lauded worldwide, especially in Australia, where the press reported on it intensively. This paper reconstructs the story of the flight by analysing the Australian press accounts and De Pinedo's diary, to understand how the Australian public perceived the event. It investigates the aviators’ arrival in Perth, Adelaide and Melbourne, where their popularity was greatest and where the local Italian communities enthusiastically welcomed them. The analysis shows that the flight engendered increased public interest and paid dividends in terms of image for the commercial companies involved, while fascism exploited it to display its progress in aviation. The paper concludes by exploring the legacy of the endeavour in modern Italy and Australia, emphasising the differences between the countries.
A perfect symbiosis: Curaçao, the Netherlands and financial offshore services, 1951–2013
Authors: van Beurden Tijn , Jonker Joost
Published: January 2021

Analysing Curaçao as an offshore financial centre from its inception to its gradual decline, we find that it originated and evolved in close concert with the demand for such services from Western countries. Dutch banks and multinationals spearheaded the creation of institutions on the island facilitating tax avoidance. In this they were aided and abetted by their government, which firmly supported the Antilles in getting access to bilateral tax treaties, notably the one with the United States. Until the mid 1980s Curaçao flourished, but then found it increasingly difficult to keep a competitive advantage over other offshore centres. Meanwhile the Curaçao connection had enabled the Netherlands to turn itself into a hub for international revenue flows that today still feed both Dutch tax income and specialised financial, legal and accounting services.
Cobra-head and other shape-memory abnormalities of nitinol atrial septal occluders: incidence, predisposing factors, and outcomes
Authors: Sonawane Bhushan S. , Sagar Pramod , Mohakud Asish R. , Rohitraj Gopalavilasam R. , Wadile Santosh , Sivakumar Kothandam
Published: January 2021

Background:

Shape-memory abnormalities are seen in some nitinol atrial septal occluders. Variably described as cobra-head, tulip, and others, their incidence, mechanisms, clinical impact, and outcome have not been systematically analysed.

Methods:

We retrospectively reviewed all consecutive device closures in the last 6 years for deformations. Type and size of the occluder, deployment technique, size, and angulation/kinking of the delivery sheath were analysed. Procedural success, duration, and other complications were studied.

Results:

A total of 112 devices (11.8%) among 950 occluders used in 936 patients showed deformities. Fourteen of 936 received 2 devices. Deformities were transient and self-correcting in 40%. Multivariate analysis showed significant associations with oversized sheaths (p = 0.004), kinked/angulated sheaths (p < 0.001), special deployment techniques (p < 0.001), and twist in the device waist (p = 0.011). Despite more frequent deformities with Figulla (15.6%) and Amplatzer (13.9%) occluders than Cera occluders (6.6%) and larger devices (>24 mm – 14.6%) than smaller devices (less than or equal to 24 mm – 9.7%), they were not significant on multivariate analysis. In vivo manipulations corrected most deformities; nineteen needed in vitro reformations and four needed a change of device. Despite prolongation of the procedure, repeated attempts (mean 2.76 ± 1.7 attempts, with a range from 1 to 9 attempts), and supraventricular tachycardia in two patients, there were no serious adverse effects.

Conclusions:

Deformations were frequent in 11.8% of atrial septal occluders on a targeted search. Oversized and angulated/kinked sheaths, special techniques like pulmonary vein deployment and twist in device waist during procedure predisposed to deformities. While most deformities were corrected with manipulations, removal of the device was infrequently needed and change of device was rarely required. Long procedural time and multiple attempts for deployment did not affect procedural success.
The Impact of Memory Change on Everyday Life Among Older Adults: Association with Cognition and Self-Reported Memory
Authors: Shaikh Komal T. , Tatham Erica L. , Vandermorris Susan , Paterson Theone , Stokes Kathryn , Freedman Morris , Levine Brian , Rich Jill B. , Troyer Angela K.
Published: January 2021

Objectives:

Many older adults experience memory changes that can have a meaningful impact on their everyday lives, such as restrictions to lifestyle activities and negative emotions. Older adults also report a variety of positive coping responses that help them manage these changes. The purpose of this study was to determine how objective cognitive performance and self-reported memory are related to the everyday impact of memory change.

Methods:

We examined these associations in a sample of 94 older adults (age 60–89, 52% female) along a cognitive ability continuum from normal cognition to mild cognitive impairment.

Results:

Correlational analyses revealed that greater restrictions to lifestyle activities (|rs| = .36–.66), more negative emotion associated with memory change (|rs| = .27–.76), and an overall greater burden of memory change on everyday living (|rs| = .28–.61) were associated with poorer objective memory performance and lower self-reported memory ability and satisfaction. Performance on objective measures of executive attention was unrelated to the impact of memory change. Self-reported strategy use was positively related to positive coping with memory change (|r| = .26), but self-reported strategy use was associated with more negative emotions regarding memory change (|r| = .23).

Conclusions:

Given the prevalence of memory complaints among older adults, it is important to understand the experience of memory change and its impact on everyday functioning in order to develop services that target the specific needs of this population.
The Agadir Agreement: The Capability Traps of Isomorphic Mimicry
Authors: Kourtelis Christos
Published: January 2021

In 2004, Morocco, Tunisia, Egypt, and Jordan signed the Agadir Agreement (AA), a free trade agreement with intention of encouraging closer cooperation in trade. The AA came into force in 2007 and relies on the EU's rules of origin. Contrary to existing explanations, which suggest that the little impact of the AA on intraregional trade is a result of the local political elites in the agreement and of weak state institutions, this article amends the concept of isomorphic mimicry to shed some light on the ineffectiveness of the AA. It claims that instead of acting as a vehicle for regional integration, the AA generated two capability traps: premature load bearing and the reproduction of the structural weaknesses of Arab Mediterranean economies. As a result, the AA does not act as an instrument of intraregional cooperation and inclusive growth.
Diabatic heating governs the seasonality of the Atlantic Niño
Authors: Nnamchi Hyacinth C. , Latif Mojib , Keenlyside Noel S. , Kjellsson Joakim , Richter Ingo
Published: January 2021

The Atlantic Niño is the leading mode of interannual sea-surface temperature (SST) variability in the equatorial Atlantic and assumed to be largely governed by coupled ocean-atmosphere dynamics described by the Bjerknes-feedback loop. However, the role of the atmospheric diabatic heating, which can be either an indicator of the atmosphere’s response to, or its influence on the SST, is poorly understood. Here, using satellite-era observations from 1982–2015, we show that diabatic heating variability associated with the seasonal migration of the Inter-Tropical Convergence Zone controls the seasonality of the Atlantic Niño. The variability in precipitation, a measure of vertically integrated diabatic heating, leads that in SST, whereas the atmospheric response to SST variability is relatively weak. Our findings imply that the oceanic impact on the atmosphere is smaller than previously thought, questioning the relevance of the classical Bjerknes-feedback loop for the Atlantic Niño and limiting climate predictability over the equatorial Atlantic sector.
Structure of a full-length bacterial polysaccharide co-polymerase
Authors: Wiseman Benjamin , Nitharwal Ram Gopal , Widmalm Göran , Högbom Martin
Published: January 2021

Lipopolysaccharides are important components of the bacterial cell envelope that among other things act as a protective barrier against the environment and toxic molecules such as antibiotics. One of the most widely disseminated pathways of polysaccharide biosynthesis is the inner membrane bound Wzy-dependent pathway. Here we present the 3.0 Å structure of the co-polymerase component of this pathway, WzzB from E. coli solved by single-particle cryo-electron microscopy. The overall architecture is octameric and resembles a box jellyfish containing a large bell-shaped periplasmic domain with the 2-helix transmembrane domain from each protomer, positioned 32 Å apart, encircling a large empty transmembrane chamber. This structure also reveals the architecture of the transmembrane domain, including the location of key residues for the Wzz-family of proteins and the Wzy-dependent pathway present in many Gram-negative bacteria, explaining several of the previous biochemical and mutational studies and lays the foundation for future investigations.
From Ru-bda to Ru-bds: a step forward to highly efficient molecular water oxidation electrocatalysts under acidic and neutral conditions
Authors: Yang Jing , Wang Lei , Zhan Shaoqi , Zou Haiyuan , Chen Hong , Ahlquist Mårten S. G. , Duan Lele , Sun Licheng
Published: January 2021

Significant advances during the past decades in the design and studies of Ru complexes with polypyridine ligands have led to the great development of molecular water oxidation catalysts and understanding on the O−O bond formation mechanisms. Here we report a Ru-based molecular water oxidation catalyst [Ru(bds)(pic)2] (Ru-bds; bds2− = 2,2′-bipyridine-6,6′-disulfonate) containing a tetradentate, dianionic sulfonate ligand at the equatorial position and two 4-picoline ligands at the axial positions. This Ru-bds catalyst electrochemically catalyzes water oxidation with turnover frequencies (TOF) of 160 and 12,900 s−1 under acidic and neutral conditions respectively, showing much better performance than the state-of-art Ru-bda catalyst. Density functional theory calculations reveal that (i) under acidic conditions, the high valent Ru intermediate RuV=O featuring the 7-coordination configuration is involved in the O−O bond formation step; (ii) under neutral conditions, the seven-coordinate RuIV=O triggers the O−O bond formation; (iii) in both cases, the I2M (interaction of two M−O units) pathway is dominant over the WNA (water nucleophilic attack) pathway.
Salivary cortisol is associated with cognitive changes in patients with fibromyalgia
Authors: Lin Yi-Ju , Ko Yu-Chieh , Chow Lok-Hi , Hsiao Fu-Jung , Liu Hung-Yu , Wang Pei-Ning , Chen Wei-Ta
Published: January 2021

Fibromyalgia (FM) is a stress-related chronic pain disorder with common cognitive complaints. This study characterized cognitive dysfunction in patients with FM and explored whether these changes are linked to altered cortisol levels. Consecutive 44 patients with FM and 48 healthy controls were enrolled for the assessments of subjective and objective cognitive functions and diurnal levels of salivary cortisol (sampled at awakening, 30 min after awakening, 3 pm, and bedtime). All measurements were compared between the groups and evaluated for clinical correlation. The FM group had more subjective cognitive complaints and performed poorer in objective cognitive testing in memory (delayed recall in Chinese Version Verbal Learning Test and Taylor Complex Figure Test), language (Boston Naming Test), and executive domains (Wisconsin Card Sorting Test) after adjustments for education. The diurnal cortisol levels of patients with FM tended to be lower, especially at 30 min after awakening and bedtime. Moreover, moderate positive correlations existed between the Chinese Version Verbal Learning Test, Boston Naming Test and the morning cortisol levels within the FM group. We suggested the altered cognitive function in FM may be linked to stress maladaptation. Future studies are warranted to elucidate whether stress management improves cognitive performance in patients with FM.
The adverse events of haematopoietic stem cell transplantation are associated with gene polymorphism within human leukocyte antigen region
Authors: Chen Ding-Ping , Wen Ying-Hao , Wang Po-Nan , Hour Ai-Ling , Lin Wei-Tzu , Hsu Fang-Ping , Wang Wei-Ting
Published: January 2021

Adverse reactions may still occur in some patients after receiving haematopoietic stem cell transplantation (HSCT), even when choosing a human leukocyte antigen (HLA)-matched donor. The adverse reactions of transplantation include disease relapse, graft-versus-host disease (GVHD), mortality and CMV infection. However, only the relapse was discussed in our previous study. Therefore, in this study, we investigated the correlation between the gene polymorphisms within the HLA region and the adverse reactions of post-HSCT in patients with acute leukaemia (n = 176), where 72 patients were diagnosed with acute lymphocytic leukaemia (ALL) and 104 were acute myeloid leukaemia (AML). The candidate single nucleotide polymorphisms were divided into three models: donor, recipient, and donor-recipient pairs and the data of ALL and AML were analysed individually. Based on the results, we found 16 SNPs associated with the survival rates, the risk of CMV infection, or the grade of GVHD in either donor, recipient, or donor-recipient matching models. In the ALL group, the rs209132 of TRIM27 in the donor group was related to CMV infection (p = 0.021), the rs213210 of RING1 in the recipient group was associated with serious GVHD (p = 0.003), and the rs2227956 of HSPA1L in the recipient group correlated with CMV infection (p = 0.001). In the AML group, the rs3130048 of BAG6 in the donor-recipient pairs group was associated with serious GVHD (p = 0.048). Moreover, these SNPs were further associated with the duration time of survival after transplantation. These results could be applied to select the best donor in HSCT.
Altered asymmetries of the structural networks comprising the fronto-limbic brain circuitry of preterm infants
Authors: Lee Joo Young , Choi Yong-Ho , Cha Jong Ho , Lee Ji Young , Lee Young-Jun , Kim Bo-Hyun , Kim Il-Kewon , Lee Jong-Min , Lee Hyun Ju
Published: January 2021

This study aimed to elaborate upon prior findings suggestive of the altered lateralization of structural connectivity in the developing preterm brain by using diffusion tensor imaging tractography to explore how network topological asymmetries in fronto-limbic neural circuitry are altered at 36–41 weeks, postmenstrual age in 64 preterm infants without severe brain injury and 33 term-born infants. We compared the pattern of structural connectivity and network lateralization of the betweenness centrality in the medial fronto-orbital gyrus, superior temporal gyrus, amygdala, and hippocampus—the structures comprising the fronto-limbic brain circuit—between preterm and term infants. Global efficiency, local efficiency, and small-world characteristics did not differ significantly between the two hemispheres in term-born infants, suggesting that integration and segregation are balanced between the left and right hemispheres. However, the preterm brain showed significantly greater leftward lateralization of small-worldness (P = 0.033); the lateralization index of the betweenness centrality revealed that the medial fronto-orbital gyrus (P = 0.008), superior temporal gyrus (P = 0.031), and hippocampus (P = 0.028) showed significantly increased leftward asymmetry in preterm infants relative to term-infants independent of sex, age at imaging, and bronchopulmonary dysplasia. The altered lateralization of fronto-limbic brain circuitry might be involved in the early development of social–emotional disorders in preterm infants.
Competition between cyclization and unusual Norrish type I and type II nitro-acyl migration pathways in the photouncaging of 1-acyl-7-nitroindoline revealed by computations
Authors: Morgante Pierpaolo , Guruge Charitha , Ouedraogo Yannick P. , Nesnas Nasri , Peverati Roberto
Published: January 2021

The 7-nitroindolinyl family of caging chromophores has received much attention in the past two decades. However, its uncaging mechanism is still not clearly understood. In this study, we performed state-of-the-art density functional theory calculations to unravel the photo-uncaging mechanism in its entirety, and we compared the probabilities of all plausible pathways. We found competition between a classical cyclization and an acyl migration pathway, and here we explain the electronic and steric reasons behind such competition. The migration mechanism possesses the characteristics of a combined Norrish type I and a 1,6-nitro-acyl variation of a Norrish type II mechanism, which is reported here for the first time. We also found negligible energetic differences in the uncaging mechanisms of the 4-methoxy-5,7-dinitroindolinyl (MDNI) cages and their mononitro analogues (MNI). We traced the experimentally observed improved quantum yields of MDNI to a higher population of the reactants in the triplet surface. This fact is supported by a more favorable intersystem crossing due to the availability of a higher number of triplet excited states with the correct symmetry in MDNI than in MNI. Our findings may pave the way for improved cage designs that possess higher quantum yields and a more efficient agonist release.
Increased VEGF-A in solid type of lung adenocarcinoma reduces the patients’ survival
Authors: Jung Woon Yong , Min Kyueng-Whan , Oh Young Ha
Published: January 2021

The histological classification of lung adenocarcinoma includes 5 types: lepidic, acinar, papillary, micropapillary and solid. The complex gene interactions and anticancer immune response of these types are not well known. The aim of this study was to reveal the survival rates, genetic alterations and immune activities of the five histological types and provide treatment strategies. This study reviewed the histological findings of 517 patients with lung adenocarcinoma from The Cancer Genome Atlas (TCGA) database and classified them into five types. We performed gene set enrichment analysis (GSEA) and survival analysis according to the different types. We found six oncogenic gene sets that were higher in lung adenocarcinoma than in normal tissues. In the survival analysis of each type, the acinar type had a favorable prognosis, and the solid subtype had an unfavorable prognosis; however, the survival differences between the other types were not significant. Our study focused on the solid type, which had the poorest prognosis. The solid type was related to adaptive immune resistance associated with elevated CD8 T cells and high CD274 (encoding PD-L1) expression. In the pathway analyses, the solid type was significantly related to high vascular endothelial growth factor (VEGF)-A expression, reflecting tumor angiogenesis. Non-necrosis/low immune response affected by high VEGF-A was associated with worse prognosis. The solid type associated with high VEGF-A expression may contribute to the development of therapeutic strategies for lung adenocarcinoma.
Nuclear resonance fluorescence drug inspection
Authors: Lan Haoyang , Song Tan , Huang Xingde , Zhao Shengqiang , Zhou Jianliang , Zhu Zhichao , Xu Yi , Balabanski Dimiter L. , Luo Wen
Published: January 2021

There is an increasing challenge to prevent illicit drug smuggling across borders and seaports. However, the existing techniques in-and-of-themselves are not sufficient to identify the illicit drugs rapidly and accurately. In the present study, combining nuclear resonance fluorescence (NRF) spectroscopy and the element (or isotope) ratio approach, we present a novel inspection method that can simultaneously reveal the elemental (or isotopic) composition of the illicit drugs, such as widely abused methamphetamine, cocaine, heroin, ketamine and morphine. In the NRF spectroscopy, the nuclei are excited by the induced photon beam, and measurement of the characteristic energies of the emitted $$\gamma $$
γ
rays from the distinct energy levels in the excited nuclei provides “fingerprints” of the interested elements in the illicit drugs. The element ratio approach is further used to identify drug elemental composition in principle. Monte Carlo simulations show that four NRF peaks from the nuclei $$^{12}$$

12

C, $$^{14}$$

14

N and $$^{16}$$

16

O can be detected with high significance of 7−24$$\sigma $$
σ
using an induced photon beam flux of $$10^{11}$$

10
11

. The ratio of $$^{14}N$$

14

N

/$$^{12}C$$

12

C

and/or $$^{16}O$$

16

O

/$$^{12}C$$

12

C

for illicit drugs inspected are then extracted using the element ratio approach. It is found that the present results of simulations are in good agreement with the theoretical calculations. The feasibility to detect the illicit drugs, inside the 15-mm-thick iron shielding, or surrounded by thin benign materials, is also discussed. It is indicated that, using the state-of-the-art $$\gamma $$
γ
-ray source of high intensity and energy-tunability, the proposed method has a great potential for identifying drugs and explosives in a realistic measurement time.
Autophagy facilitates type I collagen synthesis in periodontal ligament cells
Authors: Nakamura Tomomi , Yamashita Motozo , Ikegami Kuniko , Suzuki Mio , Yanagita Manabu , Kitagaki Jirouta , Kitamura Masahiro , Murakami Shinya
Published: January 2021

Autophagy is a lysosomal protein degradation system in which the cell self-digests its intracellular protein components and organelles. Defects in autophagy contribute to the pathogenesis of age-related chronic diseases, such as myocardial infarction and rheumatoid arthritis, through defects in the extracellular matrix (ECM). However, little is known about autophagy in periodontal diseases characterised by the breakdown of periodontal tissue. Tooth-supportive periodontal ligament (PDL) tissue contains PDL cells that produce various ECM proteins such as collagen to maintain homeostasis in periodontal tissue. In this study, we aimed to clarify the physiological role of autophagy in periodontal tissue. We found that autophagy regulated type I collagen synthesis by elimination of misfolded proteins in human PDL (HPDL) cells. Inhibition of autophagy by E-64d and pepstatin A (PSA) or siATG5 treatment suppressed collagen production in HPDL cells at mRNA and protein levels. Immunoelectron microscopy revealed collagen fragments in autolysosomes. Accumulation of misfolded collagen in HPDL cells was confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. E-64d and PSA treatment suppressed and rapamycin treatment accelerated the hard tissue-forming ability of HPDL cells. Our findings suggest that autophagy is a crucial regulatory process that facilitates type I collagen synthesis and partly regulates osteoblastic differentiation of PDL cells.
Artemether ameliorates kidney injury by restoring redox imbalance and improving mitochondrial function in Adriamycin nephropathy in mice
Authors: Han Pengxun , Cai Yuchun , Wang Yao , Weng Wenci , Chen Yinghui , Wang Menghua , Zhan Hongyue , Yu Xuewen , Wang Taifen , Shao Mumin , Sun Huili
Published: January 2021

The kidney is a high-energy demand organ rich in mitochondria especially renal tubular cells. Emerging evidence suggests that mitochondrial dysfunction, redox imbalance and kidney injury are interconnected. Artemether has biological effects by targeting mitochondria and exhibits potential therapeutic value for kidney disease. However, the underlying molecular mechanisms have not been fully elucidated. This study was performed to determine the effects of artemether on Adriamycin-induced nephropathy and the potential mechanisms were also investigated. In vivo, an Adriamycin nephropathy mouse model was established, and mice were treated with or without artemether for 2 weeks. In vitro, NRK-52E cells were stimulated with TGF-β1 and treated with or without artemether for 24 h. Then renal damage and cell changes were evaluated. The results demonstrated that artemether reduced urinary protein excretion, recovered podocyte alterations, attenuated pathological changes and alleviated renal tubular injury. Artemether also downregulated TGF-β1 mRNA expression levels, inhibited tubular proliferation, restored tubular cell phenotypes and suppressed proliferation-related signalling pathways. In addition, artemether restored renal redox imbalance, increased mtDNA copy number and improved mitochondrial function. In summary, we provided initial evidence that artemether ameliorates kidney injury by restoring redox imbalance and improving mitochondrial function in Adriamycin nephropathy in mice. Artemether may be a promising agent for the treatment kidney disease.
Intermittency in phytoplankton bloom triggered by modulations in vertical stability
Authors: Keerthi Madhavan Girijakumari , Lévy Marina , Aumont Olivier
Published: January 2021

Seasonal surface chlorophyll (SChl) blooms are very chaotic in nature, but traditional bloom paradigms have climbed out of these subseasonal variations. Here we highlight the leading order role of wind bursts, by conjoining two decades of satellite SChl with atmospheric reanalysis in the Northwestern Mediterranean Sea. We demonstrate that weekly SChl fluctuations are in phase with weekly changes in wind stress and net heat flux during the intial state of the bloom in winter and early spring, thus expanding the convection shutdown hypothesis of bloom onset to subseasonal timescales. We postulate that the mechanism reflected by this link is intermittency in vertical stability due to short-term episodes of calm weather in winter or to stormy conditions in early spring, leading to short-term variations in light exposure or to events of vertical dilution. This strong intermittency in phytoplankton bloom may probably have important consequences on carbon export and trophic web structure and should not be overlooked.
Decreased levels of circulating cytokines VEGF, TNF-β and IL-15 indicate PD-L1 overexpression in tumours of primary breast cancer patients
Authors: Cierna Zuzana , Smolkova Bozena , Cholujova Dana , Gronesova Paulina , Miklikova Svetlana , Cihova Marina , Plava Jana , Mego Michal
Published: January 2021

Programmed death ligand 1 (PD-L1) overexpression has been associated with poor clinical outcomes in several human cancers whose increased malignant behaviour might be related to PD-L1 mediated systemic immunological tolerance. This study aims to verify if circulating cytokines may serve as a proxy for non-invasive identification of sensitive prognostic biomarkers reflecting tumour and its microenvironment. Immunohistochemistry was used to measure PD-L1 expression in tumour tissue sections of 148 chemonaïve breast cancer (BC) patients. The panel of 51 cytokines was analysed using multiplex bead arrays. High PD-L1 expression in tumours was associated with shorter progression-free survival (HR 3.25; 95% CI 1.39–7.61; P = 0.006) and low circulating levels of three multifunctional molecules; VEGF, TNF-β and IL-15 (P = 0.001). In multivariate analysis, patients with low VEGF had 4.6-fold increased risk of PD-L1 overexpression (P = 0.008), present in 76.5% of patients with all these three cytokines below the median (vs. 35.6% among the others; P = 0.002). The area under the curve value of 0.722 (95% CI 0.59–0.85; P = 0.004) shows that this combination of cytokines has a moderate ability to discriminate between PD-L1 high vs. PD-L1 low patients. Plasma cytokines, therefore, could serve as potential non-invasive biomarkers for the identification of high-risk BC cases.
Stochastic events can explain sustained clustering and polarisation of opinions in social networks
Authors: Condie Scott A. , Condie Corrine M.
Published: January 2021

Understanding the processes underlying development and persistence of polarised opinions has been one of the key challenges in social networks for more than two decades. While plausible mechanisms have been suggested, they assume quite specialised interactions between individuals or groups that may only be relevant in particular contexts. We propose that a more broadly relevant explanation might be associated with the influence of external events. An agent-based bounded-confidence model has been used to demonstrate persistent polarisation of opinions within populations exposed to stochastic events (of positive and negative influence) even when all interactions between individuals are noisy and assimilative. Events can have a large impact on the distribution of opinions because their influence acts synchronistically across a large proportion of the population, whereas an individual can only interact with small numbers of other individuals at any particular time.
Parents with periodontitis impact the subgingival colonization of their offspring
Authors: Monteiro Mabelle Freitas , Altabtbaei Khaled , Kumar Purnima S. , Casati Márcio Zaffalon , Ruiz Karina Gonzales Silverio , Sallum Enilson Antonio , Nociti-Junior Francisco Humberto , Casarin Renato Corrêa Viana
Published: January 2021

Early acquisition of a pathogenic microbiota and the presence of dysbiosis in childhood is associated with susceptibility to and the familial aggregation of periodontitis. This longitudinal interventional case–control study aimed to evaluate the impact of parental periodontal disease on the acquisition of oral pathogens in their offspring. Subgingival plaque and clinical periodontal metrics were collected from 18 parents with a history of generalized aggressive periodontitis and their children (6–12 years of age), and 18 periodontally healthy parents and their parents at baseline and following professional oral prophylaxis. 16S rRNA amplicon sequencing revealed that parents were the primary source of the child's microbiome, affecting their microbial acquisition and diversity. Children of periodontitis parents were preferentially colonized by Filifactor alocis, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Streptococcus parasanguinis, Fusobacterium nucleatum and several species belonging to the genus Selenomonas even in the absence of periodontitis, and these species controlled inter-bacterial interactions. These pathogens also emerged as robust discriminators of the microbial signatures of children of parents with periodontitis. Plaque control did not modulate this pathogenic pattern, attesting to the microbiome's resistance to change once it has been established. This study highlights the critical role played by parental disease in microbial colonization patterns in their offspring and the early acquisition of periodontitis-related species and underscores the need for greater surveillance and preventive measures in families of periodontitis patients.
The role of the brown bear Ursus arctos as a legitimate megafaunal seed disperser
Authors: García-Rodríguez Alberto , Albrecht Jörg , Szczutkowska Sylwia , Valido Alfredo , Farwig Nina , Selva Nuria
Published: January 2021

Megafaunal frugivores can consume large amounts of fruits whose seeds may be dispersed over long distances, thus, affecting plant regeneration processes and ecosystem functioning. We investigated the role of brown bears (Ursus arctos) as legitimate megafaunal seed dispersers. We assessed the quantity component of seed dispersal by brown bears across its entire distribution based on information about both the relative frequency of occurrence and species composition of fleshy fruits in the diet of brown bears extracted from the literature. We assessed the quality component of seed dispersal based on germination experiments for 11 fleshy-fruited plant species common in temperate and boreal regions and frequently eaten by brown bears. Across its distribution, fleshy fruits, on average, represented 24% of the bear food items and 26% of the total volume consumed. Brown bears consumed seeds from at least 101 fleshy-fruited plant species belonging to 24 families and 42 genera, of which Rubus (Rosaceae) and Vaccinium (Ericaceae) were most commonly eaten. Brown bears inhabiting Mediterranean forests relied the most on fleshy fruits and consumed the largest number of species per study area. Seeds ingested by bears germinated at higher percentages than those from whole fruits, and at similar percentages than manually depulped seeds. We conclude that brown bears are legitimate seed dispersers as they consume large quantities of seeds that remain viable after gut passage. The decline of these megafaunal frugivores may compromise seed dispersal services and plant regeneration processes.
Spatiotemporal drought analysis by the standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI) in Sichuan Province, China
Authors: Liu Changhong , Yang Cuiping , Yang Qi , Wang Jiao
Published: January 2021

Drought refers to a meteorological disaster that causes insufficient soil moisture and damage to crop water balance due to long-term lack of precipitation. With the increasing shortage of water resources, drought has become one of the hot issues of global concern. The standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI) can effectively reflect the changes in drought characteristics of different geomorphologies in Sichuan on time and space scales, to explore the difference in drought characteristics between different physiognomy types in Sichuan Province, We calculated the SPI and SPEI values based on the data of 44 meteorological stations in Sichuan Province from 1961 to 2019 and used Mann–Kendall trend test and multivariable linear regression method (MLR) to quantify the significance of the drought characteristic trends at different time and space scales. The results as follow: (1) The SPEI drought trend in plain and hilly regions was greater than that in plateau and mountain regions on all time scales (− 0.039 year−1 for 1-month in hilly, − 0.035 year−1 for 1-month in plain, − 0.14 year−1 for 1-month in plateau, − 0.026 year−1 for 1-month in mountain) and the magnitude of trend of eastern (− 4.4 to 0.1 year−1) was lager than western (− 2.1 to 2.7 year−1), means that the drought trends transfer from northwest to east. (2) The drought intensity in the western region gradually increased (0.54–1.05) and drought events mainly occurred in the southwest plateau and central mountainous regions (24–47 times), means that drought meteorological hotspots were mainly concentrated in the Sichuan basin. (3) The MLR indicated altitude (H) is not the main influencing factor that causes the spatial unevenness of precipitation in Sichuan Province, but altitude (H), temperature (T), longitude (Lo) and latitude (La) can co-determined the precipitation. The results of this study are instructive and practical for drought assessment, risk management and application decision-making in Sichuan Province, and have guiding significance for agricultural disaster prevention, mitigation and agricultural irrigation in Sichuan Province.
Fungus Metarhizium robertsii and neurotoxic insecticide affect gut immunity and microbiota in Colorado potato beetles
Authors: Kryukov Vadim Yu. , Rotskaya Ulyana , Yaroslavtseva Olga , Polenogova Olga , Kryukova Natalia , Akhanaev Yuriy , Krivopalov Anton , Alikina Tatyana , Vorontsova Yana L. , Slepneva Irina , Kabilov Marsel , Glupov Viktor V.
Published: January 2021

Fungal infections and toxicoses caused by insecticides may alter microbial communities and immune responses in the insect gut. We investigated the effects of Metarhizium robertsii fungus and avermectins on the midgut physiology of Colorado potato beetle larvae. We analyzed changes in the bacterial community, immunity- and stress-related gene expression, reactive oxygen species (ROS) production, and detoxification enzyme activity in response to topical infection with the M. robertsii fungus, oral administration of avermectins, and a combination of the two treatments. Avermectin treatment led to a reduction in microbiota diversity and an enhancement in the abundance of enterobacteria, and these changes were followed by the downregulation of Stat and Hsp90, upregulation of transcription factors for the Toll and IMD pathways and activation of detoxification enzymes. Fungal infection also led to a decrease in microbiota diversity, although the changes in community structure were not significant, except for the enhancement of Serratia. Fungal infection decreased the production of ROS but did not affect the gene expression of the immune pathways. In the combined treatment, fungal infection inhibited the activation of detoxification enzymes and prevented the downregulation of the JAK-STAT pathway caused by avermectins. The results of this study suggest that fungal infection modulates physiological responses to avermectins and that fungal infection may increase avermectin toxicosis by blocking detoxification enzymes in the gut.
Differentially expressed lncRNAs in liver tissues of TX mice with hepatolenticular degeneration
Authors: Zhang Juan , Ma Ying , Xie Daojun , Bao Yuancheng , Yang Wenming , Wang Han , Jiang Huaizhou , Han Hui , Dong Ting
Published: January 2021

Wilson's Disease (WD), an ATP7B-mutated inherited disease that affects copper transport, is characterised by liver and nervous system manifestations. Long non-coding (ln-c) RNAs are widely involved in almost all physiological and pathological processes in the body, and are associated with numerous diseases. The present study aimed to elucidate the lncRNA-mRNA regulation network in a TX WD mouse model using RNA sequencing (RNA-seq). lncRNA expression profiles were screened using RNA-seq and real-time polymerase chain reaction, and differentially expressed lncRNAs and mRNAs were identified. To analyse the biological functions and pathways for the differentially expressed mRNAs, gene ontology and pathway enrichment analyses were performed. A significantly correlated lncRNA-mRNA relationship pair was calculated by CNC analysis to construct differential lncRNA and mRNA co-expression networks. A total of 2564 significantly up-regulated and 1052 down-regulated lncRNAs, and 1576 up-regulated and 297 down-regulated mRNAs, were identified. These genes were found to be associated with key processes such as apoptosis, and KEGG analysis revealed enrichment in the drug metabolism-cytochrome P450 pathway, PPAR signalling pathway, Notch signalling pathway, and MAPK signalling pathway. The identified differential lncRNAs may be involved in the pathogenesis and development of WD liver injury.