bluuebunny/crossref_metadata_2025 huggingface dataset:
Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead
Authors: Zhu De-long , Guo Yan , Ma Bing-chan , Lin Yong-qin , Wang Hai-jun , Gao Chao-xian , Liu Ming-qi , Zhang Nai-xing , Luo Hao , Hui Chang-ye
Published: July 2023

With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detection of toxic heavy metals in environmental water. However, most proposed biosensors are based on fluorescent and bioluminescent signals invisible to the naked eye. The development of visible pigment-based biosensors can address this issue. The pbr operon from Klebsiella pneumoniae is selectively induced by bioavailable Pb(II). In the present study, the proviolacein biosynthetic gene cluster was transcriptionally fused to the pbr Pb(II) responsive element and introduced into Escherichia coli. The resultant biosensor responded to Pb(II) in a time- and dose-dependent manner. After a 5-h incubation with Pb(II), the brown pigment was produced, which could be extracted into n-butanol. Extra hydrogen peroxide treatment during n-butanol extract resulted in the generation of a stable green pigment. An increased brown signal was observed upon exposure to lead concentrations above 2.93 nM, and a linear regression was fitted from 2.93 to 3,000 nM. Extra oxidation significantly decreased the difference between parallel groups. The green signal responded to as low as 0.183 nM Pb(II), and a non-linear regression was fitted in a wide concentration range from 0.183 to 3,000 nM. The specific response toward Pb(II) was not interfered with by various metals except for Cd(II) and Hg(II). The PV-based biosensor was validated in monitoring bioaccessible Pb(II) spiked into environmental water. The complex matrices did not influence the regression relationship between spiked Pb(II) and the dual-color signals. Direct reading with the naked eye and colorimetric quantification enable the PV-based biosensor to be a dual-color and low-cost bioindicator for pollutant heavy metal.
Postmortem skin microbiome signatures associated with human cadavers within the first 12 h at the morgue
Authors: Iancu Lavinia , Muslim Azdayanti , Aazmi Shafiq , Jitaru Victor
Published: July 2023

Introduction

Forensic microbiome studies expanded during the last decade, aiming to identify putative bacterial biomarkers to be used for the postmortem interval (PMI) estimation. Bacterial diversity and dynamics during decomposition are influenced by each individual’s micro and macroenvironment, ante and postmortem conditions, varying across body sites and time. The skin, the largest organ of the human body, hosts a diverse microbial diversity, representing the first line of defense of a living individual. Targeting the investigation of the postmortem skin microbiome could help understanding the role of microbes during decomposition, and association with the ante and postmortem conditions.

Methods

The current study aimed to identify the postmortem skin microbiome signatures associated with eight human bodies, received at the Institute of Legal Medicine Iasi, Romania, during April and May 2021. A total of 162 samples (including triplicate) representing face and hands skin microbiome were investigated via Illumina MiSeq, upon arrival at the morgue (T0) and after 12 hours (T1).

Results

The taxonomic characteristics of the skin microbiota varied across different body sites. However, there were no significant differences in taxonomic profiles between collection time, T0 and T1, except for some dynamic changes in the abundance of dominant bacteria. Moreover, different microbial signatures have been associated with a specific cause of death, such as cardiovascular disease, while an elevated blood alcohol level could be associated with a decrease in bacterial richness and diversity.

Discussion

The places where the bodies were discovered seemed to play an important role in explaining the bacterial diversity composition. This study shows promising results towards finding common postmortem bacterial signatures associated with human cadavers within the first 12h at the morgue.
A multivariate approach to explore the volatolomic and sensory profiles of craft Italian Grape Ale beers produced with novel Saccharomyces cerevisiae strains
Authors: Pietrafesa Rocchina , Siesto Gabriella , Tufariello Maria , Palombi Lorenzo , Baiano Antonietta , Gerardi Carmela , Braghieri Ada , Genovese Francesco , Grieco Francesco , Capece Angela
Published: July 2023

This study investigated the influence of three Saccharomyces cerevisiae strains, selected from different matrices - CHE-3 (cherry), P4 (sourdough) and TA4-10 (grape must) - on characteristics of Italian Grape Ale (IGA) beers obtained at microbrewery scale. A multidisciplinary approach, combining results from analysis of chemical, volatile and organoleptic profiles of the beers, was adopted to underline the relationships between yeast starter and the quality of final products. Detection volatile organic compounds (VOCs) by Gas-Chromatography coupled with Mass Spectrometry (GC-MS) after extraction carried out by head-space micro-extraction (HS-SPME) revealed that the beer obtained by P4 strain differed from the others for its higher concentrations of esters, alcohols, and terpenes as confirmed by PCA (principal component analysis) and Cluster heatmap. Furthermore, sensorial analysis and consumer test showed that this sample differed from others by more pronounced notes of “fruity smell and floral” and “olfactory finesse,” and it was the most appreciated beer for smell, taste, and overall quality. Conversely, CHE-3 was the sample with the lowest concentrations of the identified volatiles and, together TA4-10, showed the highest scores for smoked, yeast, malt, and hop notes. As far as we know, these are the first results on the application of indigenous S. cerevisiae strains in the production of craft IGA beers analyzed through a complex multivariate approach.
Changes in plasma endocannabinoids concentrations correlate with 18F-FDG PET/MR uptake in brown adipocytes in humans
Authors: Maliszewska Katarzyna , Miniewska Katarzyna , Godlewski Adrian , Gosk Wioleta , Mojsak Malgorzata , Kretowski Adam , Ciborowski Michal
Published: July 2023

Introduction: Recent data suggest a possible role of endocannabinoids in the regulation of brown adipose tissue (BAT) activity. Those findings indicate potential treatment options for obesity. The aim of this study was to evaluate the relationship between plasma endocannabinoids concentrations and the presence of BAT in humans.

Methods: The study group consisted of 25 subjects divided into two groups: BAT positive BAT(+), (n = 17, median age = 25 years) and BAT negative BAT(−), (n = 8, median age = 28 years). BAT was estimated using 18F-FDG PET/MR after 2 h of cold exposure. The level of plasma endocannabinoids was assessed at baseline, 60 min and 120 min of cold exposure.

Results: In both groups, BAT(+) and BAT(−), during the cooling, we observed a decrease of the same endocannabinoids: arachidonoylethanolamide (AEA), eicosapentaenoyl ethanolamide (EPEA) and oleoyl ethanolamide (OEA) with a much more profound decline in BAT(+) subjects. Statistically significant fall of PEA (palmitoylethanolamide) and SEA (stearoylethanolamide) concentrations after 60 min (FC = 0.7, p = 0.007 and FC = 0.8, p = 0.03, respectively) and 120 min (FC = 0.81, p = 0.004, and FC = 0.9, p = 0.01, respectively) of cooling was observed only in individuals with BAT.

Conclusion: We noticed the profound decline of endocannabinoids concentrations in subjects with increased 18F-FDG PET/MR uptake in BAT. Identification of a new molecules related to BAT activity may create a new target for obesity treatment.
Muscle–Brain crosstalk in cognitive impairment
Authors: Han Xiaowei , Ashraf Muhammad , Tipparaju Srinivas M. , Xuan Wanling
Published: July 2023

Sarcopenia is an age-related, involuntary loss of skeletal muscle mass and strength. Alzheimer's disease (AD) is the most common cause of dementia in elderly adults. To date, no effective cures for sarcopenia and AD are available. Physical and cognitive impairments are two major causes of disability in the elderly population, which severely decrease their quality of life and increase their economic burden. Clinically, sarcopenia is strongly associated with AD. However, the underlying factors for this association remain unknown. Mechanistic studies on muscle–brain crosstalk during cognitive impairment might shed light on new insights and novel therapeutic approaches for combating cognitive decline and AD. In this review, we summarize the latest studies emphasizing the association between sarcopenia and cognitive impairment. The underlying mechanisms involved in muscle–brain crosstalk and the potential implications of such crosstalk are discussed. Finally, future directions for drug development to improve age-related cognitive impairment and AD-related cognitive dysfunction are also explored.
Case report: Mechanical thrombectomy for acute basilar artery occlusion via persistent hypoglossal artery
Authors: Zhang Xin , Wang Jiaxiong , Cao Zhipeng , Liu Yingtao , Dong Yi , Cheng Xin , Gao Chao , Gu Yuxiang
Published: July 2023

Persistent hypoglossal artery (PHA) is a rare carotid-vertebrobasilar anastomosis in adults. Here, we report a case of mechanical thrombectomy for acute basilar artery occlusion via the PHA. A 44-year-old man was admitted to our stroke unit with an unstable gait and aphasia for 2 h. The baseline National Institutes of Health Stroke Scale (NIHSS) score was 4, but the clinical symptoms continued to worsen. Computed tomography angiography showed the absence of the basilar artery and an abnormal anastomosis between the anterior and posterior circulation. Clinical symptoms continued to worsen, and endovascular treatment was scheduled. PHA was demonstrated and basilar artery occlusion was confirmed using digital subtraction angiography. Mechanical thrombectomy with a stent retriever and aspiration was performed via the PHA, and modified thrombolysis in cerebral infarction level 3 was achieved. The patient underwent intravenous antiplatelet therapy after the operation, and follow-up neuroimaging revealed multiple small infarcts in the cerebellum and medulla oblongata. The patient was discharged after 10 days for further rehabilitation, with an NIHSS score of 25. At 10 months follow-up, the NIHSS score decreased to 18. Recognition of this rare variation is particularly important for interventional strategy determination and rapid recanalization of basilar artery occlusion.
Integrating the latest biological advances in the key steps of a food packaging life cycle
Authors: Rzayeva Aynura , Coffigniez Fanny , Zeynalov Nizami , Gontard Nathalie , Guillard Valérie
Published: July 2023

This literature review provides a focus on the potential of integrating the latest scientific and technological advances in the biological field to improve the status of the key steps of a food packaging life cycle: production, usage, post-usage, and long-term fate. A case study of such multi-biological food packaging is demonstrated based on the use of PHAs (polyhydroxyalkanoates) polymer, a microbiologically produced polymer from non-food renewable resources, activated by the use of bioactive components to enhance its usage benefits by reducing food loss and waste, displaying potential for reusability, compostability as post-usage, and finally, being ultimately biodegradable in most common natural conditions to considerably reduce the negative impact that persistent plastics have on the environment. We discuss how designing safe and efficient multi “bio” food packaging implies finding a compromise between sometimes contradictory functional properties. For example, active antimicrobials help preserve food but can hamper the ultimate biodegradation rate of the polymer. This review presents such antagonisms as well as techniques (e.g., coatings, nanoencapsulation) and tools (e.g., release kinetic) that can help design optimized, safe, and efficient active food packaging.
Involvement in treatment decision-making and self-reported efficacy among patients with advanced colorectal cancer: a nationwide multi-center cross-sectional study
Authors: Gu Xiao-Fen , Xu Hui-Fang , Liu Yin , Li Li , Yu Yan-Qin , Zhang Xi , Wang Xiao-Hui , Wang Wen-Jun , Du Ling-Bin , Duan Shuang-Xia , Cao He-Lu , Zhao Yu-Qian , Liu Yun-Yong , Huang Juan-Xiu , Cao Ji , Fan Yan-Ping , Feng Chang-Yan , Lian Xue-Mei , Du Jing-Chang , Rezhake Remila , Ma Li , Qiao You-Lin
Published: July 2023

Introduction

This cross-sectional study evaluated the involvement of patients with advanced colorectal cancer (CRC) in treatment decision-making, assessed the treatment efficacy according to their self-reports, and investigated the influencing factors.

Methods

Patients with advanced CRC were recruited from 19 hospitals from March 2020 to March 2021 by a multi-stage multi-level sampling method. A self-designed questionnaire was used to collect demographic and clinical characteristics, involvement of CRC patients in treatment decision-making, treatment methods, and self-reported efficacy. Univariate and unordered multinomial logistic regression analyses were used to evaluate the factors affecting the involvement in treatment decision-making and self-reported efficacy.

Results

We enrolled 4533 patients with advanced CRC. The average age at diagnosis was 58.7 ± 11.8 years. For the treatment method, 32.4% of patients received surgery combined with chemotherapy, 13.1% of patients underwent surgery combined with chemotherapy and targeted therapy, and 9.7% of patients were treated with surgery alone. For treatment decision-making, 7.0% of patients were solely responsible for decision-making, 47.0% of patients shared treatment decision-making with family members, 19.0% of patients had family members solely responsible for treatment decision-making, and 27.0% of patients had their physicians solely responsible for treatment decision-making. Gender, age, education level, family income, marital status, treatment cost, hospital type, and treatment method were significantly associated with the involvement of patients in treatment decision-making. A total of 3824 patients submitted self-reported efficacy evaluations during treatment. The percentage of patients with good self-reported efficacy was 76.5% (for patients treated for the first time), 61.7% (for patients treated for the second time), and 43.2% (for patients treated after recurrence and metastasis), respectively. Occupation, education level, average annual family income, place of residence, time since cancer diagnosis, hospital type, clinical stage, targeted therapy, and involvement in treatment decision-making were the main influencing factors of self-reported efficacy of treatment.

Discussion

Conclusively, CRC patients are not highly dominant in treatment decision-making and more likely to make treatment decisions with their family and doctors. Timely and effective communication between doctors and patients can bolster patient involvement in treatment decision-making.
Assessment of bladder filling during prostate cancer radiation therapy with ultrasound and cone-beam CT
Authors: Chauhan Kiran , Ebner Daniel K. , Tzou Katherine , Ryan Karen , May Jackson , Kaleem Tasmeem , Miller Daniel , Stross William , Malouff Timothy Dean , Landy Robin , Strong Gerald , Herchko Steve , Serago Chris , Trifiletti Daniel Michael , Miller Robert Clell , Buskirk Steven , Waddle Mark R.
Published: July 2023

Prostate cancer patients undergoing external beam radiation therapy (EBRT) benefit from a full bladder to decrease bowel and bladder toxicity. Ultrasound may offer a proxy metric for evaluation, sparing CBCT dosing. Patients were prospectively enrolled pre-simulation from January 2017 to February 2018. Bladder volume was evaluated prior to RT using US daily and CBCT for three daily treatments and then weekly unless otherwise indicated. 29 patients completed median 40 days of RT, resulting in 478 CBCT and 1,099 US bladder volumes. 21 patients were treated to intact glands and 8 to the post-prostatectomy bed. Median patient age was 70 years. Bladder volume on CBCT and US positively correlated (r = 0.85), with average bladder volume for all patients of 162 mL versus 149 mL, respectively. Bladder volume during treatment was consistently lower than the volume at CT simulation (153 mL vs 194 mL, p<0.01) and progressively declined during treatment. Patients older than 70 years presented with lower average bladder volumes than those < 70 years (122 mL vs 208 mL, respectively, p<0.01). Patients with the highest agreement between CBCT and US (<10% variability) had higher average bladder volumes (192 mL vs 120 mL, p=0.01). US was found to be an accurate measure of bladder volume and may be used to monitor daily bladder volumes in patients being treated with radiation for prostate cancer.
The current status of tumor microenvironment and cancer stem cells in sorafenib resistance of hepatocellular carcinoma
Authors: Chen Siqi , Du Yaqing , Guan Xin-Yuan , Yan Qian
Published: July 2023

Hepatocellular carcinoma (HCC) is a heterogeneous and aggressive liver cancer that presents limited treatment options. Despite being the standard therapy for advanced HCC, sorafenib frequently encounters resistance, emphasizing the need to uncover the underlying mechanisms and develop effective treatments. This comprehensive review highlights the crucial interplay between the tumor microenvironment, cancer stem cells (CSCs), and epithelial-mesenchymal transition (EMT) in the context of sorafenib resistance. The tumor microenvironment, encompassing hypoxia, immune cells, stromal cells, and exosomes, exerts a significant impact on HCC progression and therapy response. Hypoxic conditions and immune cell infiltration create an immunosuppressive milieu, shielding tumor cells from immune surveillance and hindering therapeutic efficacy. Additionally, the presence of CSCs emerges as a prominent contributor to sorafenib resistance, with CD133+ CSCs implicated in drug resistance and tumor initiation. Moreover, CSCs undergo EMT, a process intimately linked to tumor progression, CSC activation, and further promotion of sorafenib resistance, metastasis, and tumor-initiating capacity. Elucidating the correlation between the tumor microenvironment, CSCs, and sorafenib resistance holds paramount importance in the quest to develop reliable biomarkers capable of predicting therapeutic response. Novel therapeutic strategies must consider the influence of the tumor microenvironment and CSC activation to effectively overcome sorafenib resistance in HCC.
Intradural fat graft packing is not indispensable in preventing postoperative cerebrospinal fluid leakage in endoscopic endonasal pituitary adenoma surgeries
Authors: Wang Xiefeng , Wang Binbin , Cheng Gang , You Yongping , Tao Chao
Published: July 2023

Objectives

Is intradural fat graft packing indispensable in preventing postoperative cerebrospinal fluid leakage in endoscopic endonasal pituitary adenoma surgeries? This study aimed to review the methods and outcomes of our graded sellar floor reconstruction strategy without fat graft packing in endoscopic endonasal pituitary adenoma surgeries.

Methods

From March 2018 to December 2022, 200 patients underwent endoscopic endonasal pituitary adenoma resection by a single author in our institute. We applied different graded skull base reconstruction strategies in different periods. Intradural fat graft packing was used to reconstruct the skull base in the early period, from March 2018 to June 2019, but fat graft was not used in the late period, from January 2020 to December 2022. The effect of these different graded skull base reconstruction strategies and whether intradural fat graft packing is necessary were evaluated by observing the incidence of postoperative cerebrospinal fluid leak.

Results

In the early period, fat graft was used to reconstruct skull base when the intraoperative cerebrospinal fluid (CSF) leakage existed. There were two patients who suffered from postoperative cerebrospinal fluid leak in this group. In the late period, fat graft was not used to reconstruct the skull base, and no patient suffered from postoperative cerebrospinal fluid leakage in this group.

Conclusions

Intradural fat graft packing is unnecessary in the endoscopic endonasal pituitary adenoma resection. The outcome of our graded sellar floor reconstruction strategy is satisfactory.
Case report: A Chinese patient with glutathione synthetase deficiency and a novel glutathione synthase mutation
Authors: Wu Xiaojiao , Jiao Jiancheng , Xia Yaofang , Yan Xiaotong , Liu Zehao , Cao Yanyan , Ma Li
Published: July 2023

Glutathione synthetase deficiency (GSSD) is an autosomal-recessive metabolic disorder caused by glutathione synthetase (GSS) gene mutations. No more than 90 cases of GSSD have been reported worldwide; thus, the spectrum of GSS mutations and the genotype–phenotype association remain unclear. Here, we present a severely affected infant carrying a compound heterozygous GSS variation, c.491G > A, and a novel variant of c.1343_1348delTACTTC. We also summarize the clinical manifestations, treatment protocol, prognosis, and genetic characteristics of previously reported GSSD cases in China. In this case study, our patient presented with tachypnea, jaundice, intractable metabolic acidosis, and hemolytic anemia. Urinary-organic acid analysis revealed elevated 5-oxoproline levels. Further, this patient showed improved outcomes owing to early diagnosis and the timely administration of vitamins C and E. Therefore, our study indicates that in clinical cases of unexplained hemolytic anemia and metabolic acidosis, GSSD should be considered. Additionally, genetic testing and antioxidant application might help identify GSSD and improve the prognosis.
Modeling stand biomass for Moso bamboo forests in Eastern China
Authors: Zhou Xiao , Yin Zixu , Zhou Yang , Zhang Xuan , Sharma Ram P. , Guan Fengying , Fan Shaohui
Published: July 2023

Stand biomass models can be used as basic decision-making tools in forest management planning. The Moso bamboo (Phyllostachys pubescens) forest, a major forest system in tropical and subtropical regions, represents a substantial carbon sink, slowing down the rise of greenhouse gas concentrations in the earth’s atmosphere. Bamboo stand biomass models are important for the assessment of the contribution of carbon to the terrestrial ecosystem. We constructed a stand biomass model for Moso bamboo using destructively sampled data from 45 sample plots that were located across the Yixing state-owned farm in Jiangsu Province, China. Among several bamboo stand variables used as predictors in the stand biomass models, mean diameter at breast height (MDBH), mean height (MH), and canopy density (CD) of bamboo contributed significantly to the model. To increase the model’s accuracy, we introduced the effects of bamboo forest block as a random effect into the model through mixed-effects modeling. The mixed-effects model described a large part of stand biomass variation (R2 = 0.6987), significantly higher than that of the ordinary least squares regression model (R2 = 0.5748). Our results show an increased bamboo stand biomass with increasing MH and CD, confirming our model’s biological logic. The proposed stand biomass model may have important management implications; for example, it can be combined with other bamboo models to estimate bamboo canopy biomass, carbon sequestration, and bamboo biomass at different growth stages.
Genome-wide association mapping of rust resistance in Aegilops longissima
Authors: Page Rae , Huang Shuyi , Ronen Moshe , Sela Hanan , Sharon Amir , Shrestha Sandesh , Poland Jesse , Steffenson Brian J.
Published: July 2023

The rust diseases, including leaf rust caused by Puccinia triticina (Pt), stem rust caused by P. graminis f. sp. tritici (Pgt), and stripe rust caused by P. striiformis f. sp. tritici (Pst), are major limiting factors in wheat production worldwide. Identification of novel sources of rust resistance genes is key to developing cultivars resistant to rapidly evolving pathogen populations. Aegilops longissima is a diploid wild grass native to the Levant and closely related to the modern bread wheat D subgenome. To explore resistance genes in the species, we evaluated a large panel of Ae. longissima for resistance to several races of Pt, Pgt, and Pst, and conducted a genome-wide association study (GWAS) to map rust resistance loci in the species. A panel of 404 Ae. longissima accessions, mostly collected from Israel, were screened for seedling-stage resistance to four races of Pt, four races of Pgt, and three races of Pst. Out of the 404 accessions screened, two were found that were resistant to all 11 races of the three rust pathogens screened. The percentage of all accessions screened that were resistant to a given rust pathogen race ranged from 18.5% to 99.7%. Genotyping-by-sequencing (GBS) was performed on 381 accessions of the Ae. longissima panel, wherein 125,343 single nucleotide polymorphisms (SNPs) were obtained after alignment to the Ae. longissima reference genome assembly and quality control filtering. Genetic diversity analysis revealed the presence of two distinct subpopulations, which followed a geographic pattern of a northern and a southern subpopulation. Association mapping was performed in the genotyped portion of the collection (n = 381) and in each subpopulation (n = 204 and 174) independently via a single-locus mixed-linear model, and two multi-locus models, FarmCPU, and BLINK. A large number (195) of markers were significantly associated with resistance to at least one of 10 rust pathogen races evaluated, nine of which are key candidate markers for further investigation due to their detection via multiple models and/or their association with resistance to more than one pathogen race. The novel resistance loci identified will provide additional diversity available for use in wheat breeding.
Close-canopy lighting, an effective energy-saving strategy for overhead sole-source LED lighting in indoor farming
Authors: Sheibani Fatemeh , Bourget Mike , Morrow Robert C. , Mitchell Cary A.
Published: July 2023

Significant advancement has been achieved improving electrical efficiency and photon efficacy of light-emitting diodes (LEDs) as the sole source of crop lighting for indoor farming. However, a significant portion of highly efficient photon emissions from improved LEDs is wasted by natural beam spread beyond cropping areas. Additional attention is needed to enhance crop-canopy photon capture efficiency (CCPCE), the fraction of photons emitted from LEDs actually incident upon foliar canopies. We postulate that by taking advantage of unique physical properties of LEDs, such as low radiant heat at photon-emitting surfaces and dimmable photon emissions, reduced vertical separation distance between light-emitting surfaces and light-receiving surfaces will enhance CCPCE by capturing more obliquely emitted photons that otherwise are lost. This “close-canopy-lighting” (CCL) strategy was tested in two ways: For an energy-efficiency strategy, LEDs were dimmed to the same photosynthetic photon flux density (PPFD) of 160 µmol m-2 s-1 at 45-, 35-, 25-, and 15-cm separation distances between lamps and cropping surfaces. For a yield-enhancement strategy, dimming was not applied, so higher PPFDs occurred at each separation distance closer than 45 cm for the same input energy. In the first strategy, the same biomass of lettuce (Lactuca sativa L. cv. Rouxai) was produced at each separation distance, while significantly lower energy was expended for lighting at each closer separation. Significantly higher biomass was produced at reduced separation distances with the same energy expenditure by LEDs using the yield-enhancement strategy. For both strategies, energy-utilization efficiency (g/kWh) doubled at the closest separation distance of 15 cm compared to the standard 45-cm separation distance. Even higher energy-utilization efficiency was achieved at a 25-cm separation distance when growth compartments were enclosed with a reflective curtain in the yield-enhancement strategy. Our findings suggest that CCL is a highly effective energy-saving strategy for overhead LED lighting, suggesting the need for innovative next-generation re-design of height-adjustable LED mounts and controlled air movement between tiers of indoor farms utilizing CCL.
TrIncNet: a lightweight vision transformer network for identification of plant diseases
Authors: Gole Pushkar , Bedi Punam , Marwaha Sudeep , Haque Md. Ashraful , Deb Chandan Kumar
Published: July 2023

In the agricultural sector, identifying plant diseases at their earliest possible stage of infestation still remains a huge challenge with respect to the maximization of crop production and farmers’ income. In recent years, advanced computer vision techniques like Vision Transformers (ViTs) are being successfully applied to identify plant diseases automatically. However, the MLP module in existing ViTs is computationally expensive as well as inefficient in extracting promising features from diseased images. Therefore, this study proposes a comparatively lightweight and improved vision transformer network, also known as “TrIncNet” for plant disease identification. In the proposed network, we introduced a modified encoder architecture a.k.a. Trans-Inception block in which the MLP block of existing ViT was replaced by a custom inception block. Additionally, each Trans-Inception block is surrounded by a skip connection, making it much more resistant to the vanishing gradient problem. The applicability of the proposed network for identifying plant diseases was assessed using two plant disease image datasets viz: PlantVillage dataset and Maize disease dataset (contains in-field images of Maize diseases). The comparative performance analysis on both datasets reported that the proposed TrIncNet network outperformed the state-of-the-art CNN architectures viz: VGG-19, GoogLeNet, ResNet-50, Xception, InceptionV3, and MobileNet. Moreover, the experimental results also showed that the proposed network had achieved 5.38% and 2.87% higher testing accuracy than the existing ViT network on both datasets, respectively. Therefore, the lightweight nature and improved prediction performance make the proposed network suitable for being integrated with IoT devices to assist the stakeholders in identifying plant diseases at the field level.
Adult’s veracity judgments of Black and White children’s statements: the role of perceiver and target race and prejudice-related concerns
Authors: Zanette Sarah , Hagi Hussein Siham , Malloy Lindsay C.
Published: July 2023

Introduction

Seldom has work investigated systematic biases in adults’ truth and lie judgments of children’s reports. Research demonstrates that adults tend to exhibit a bias toward believing a child is telling the truth, but it is unknown whether this truth bias applies equally to all children. Given the pervasiveness of racial prejudice and anti-Black racism in the United States, the current study examined whether adults are more or less likely to believe a child is telling the truth based on the race of the child (Black or White), the race of the adult perceiver (Black or White), and the perceiver’s concerns regarding appearing unprejudiced.

Methods

Using an online data-collection platform, 593 Black and White American adults reviewed fictitious vignettes in which a child denied committing a misbehavior at school (e.g., damaging a laptop). The race of the child in the vignette was manipulated using an AI-generated photo of either a Black child or a White child. After reading each story, participants provided a categorical veracity judgment by indicating whether they believed the child in the story was lying (and therefore committed the misdeed) or telling the truth (and was innocent), as well as rated how honest or deceptive the child was being on a continuous scale. Participants also completed questionnaires assessing their internal (personal) and external (normative) motivations to respond in non-prejudiced ways.

Results and discussion

Results indicated that systematic racial biases occur in adults’ veracity judgments of children’s statements. Both Black and White participants exhibited a truth bias in their veracity judgments of Black children, but not when evaluating the deceptiveness of White children. Consistent with the prejudice-related concerns hypothesis, the observed truth bias toward Black children was moderated by individual differences in participants’ desire to respond without prejudice and whether those motivations stem from external or internal sources. The current findings present novel evidence regarding racial bias and prejudice-related concerns as potential barriers to making veracity judgments of children’s statements and, ultimately, successful lie detection.
The effects of the natural visual-aural attributes of urban green spaces on human behavior and emotional response
Authors: Yin Yuting , Shao Yuhan , Meng Yu , Hao Yiying
Published: July 2023

Introduction

Nature-based solutions (NBS) have been used to address a wide range of urban environmental challenges, an important aspect of which is to improve human health and well-being. However, most relevant studies focus either on what positive influences nature may have or on identifying what natural factors can have these benefits. Few have investigated the sensory composition of nature and the effects of nature in different sensory aspects on human health. Setting out from the multi-sensory perspective, this study aims to explore human behavior and emotional response from visual and aural contact with urban nature.

Methods

Taking Jiangjia Art Garden in Chengdu as an example, natural attributes such as its visual (landscape) and aural (sound source) characteristics as well as people’s activities (behavioral responses) were measured by on-site mapping analysis. This was done while a questionnaire-based survey was conducted to investigate people’s emotional responses regarding their overall satisfaction, pleasantness, calm, and agreeableness.

Results

The results indicated that nature-dominated visual landscapes such as grassland, waterscapes, and woodlands, as well as natural sounds such as bird sounds, chirp sounds, and wind sounds were found to be positively correlated to the vitality of activities and people’s emotional status. Regarding behavioral responses, it was shown that grasslands and woodlands are more likely to be attractive places for recreation, and the vitality measured became extremely high when these two were paired with lakes. As for the emotional responses, people’s perceived overall satisfaction, calm, and agreeableness were equally reflected in their behavioral patterns, suggesting a strong relationship with natural factors.

Discussion

The research findings were visually presented in behavior and emotional maps to provide direct cues of informing the future design of high-quality urban green spaces and promoting the application of aural-visual experience in the design of urban nature areas.
Staff training in physical interventions: a literature review
Authors: McDonnell Andrew A. , O’Shea Marion C. , Bews-Pugh Stephanie J. , McAulliffe Hannah , Deveau Roy
Published: July 2023

Background

Restrictive practices are used frequently by frontline staff in a variety of care contexts, including psychiatric hospitals, children’s services, and support services for older adults and individuals with intellectual and developmental disabilities. Physical restraint has been associated with emotional harm, physical injury to staff and consumers, and has even resulted in death of individuals in care environments. Various interventions have been implemented within care settings with the intention of reducing instances of restraint. One of the most common interventions is staff training that includes some physical intervention skills to support staff to manage crisis situations. Despite physical intervention training being used widely in care services, there is little evidence to support the effectiveness and application of physical interventions. This review will examine the literature regarding outcomes of staff training in physical interventions across care sectors.

Method

A systematic search was conducted following PRISMA guidelines using Cochrane Database, Medline EBSCO, Medline OVID, PsychINFO, and the Web of Science. Main search keywords were staff training, physical intervention, physical restraint. The MMAT was utilised to provide an analytical framework for the included studies.

Results and discussion

Seventeen articles have been included in this literature review. The included studies take place in a range of care settings and comprise a wide range of outcomes and designs. The training programmes examined vary widely in their duration, course content, teaching methods, and extent to which physical skills are taught. Studies were of relatively poor quality. Many descriptions of training programmes did not clearly operationalise the knowledge and skills taught to staff. As such, it is difficult to compare course content across the studies. Few papers described physical interventions in sufficient detail. This review demonstrates that, although staff training is a ‘first response’ to managing health and safety in care settings, there is very little evidence to suggest that staff training in physical intervention skills leads to meaningful outcomes.
The mental health impact of the ongoing Russian-Ukrainian war 6 months after the Russian invasion of Ukraine
Authors: Kurapov Anton , Kalaitzaki Argyroula , Keller Vladyslava , Danyliuk Ivan , Kowatsch Tobias
Published: July 2023

Objective

This study aimed to investigate the impact of the ongoing war in Ukraine on the mental health of Ukrainians, focusing on war-induced trauma, disturbances in self-organization, post-traumatic stress disorder, complex post-traumatic stress disorder, anxiety, stress, and depression.

Methods

Data was collected from 703 participants 6 months after the full-scale invasion using a structured questionnaire that included sections on socio-demographic information, trauma-related issues, and mental health.

Results

The study found that levels of depression and anxiety were relatively low, while stress and resilience were relatively high among Ukrainians affected by the war. However, those who were directly exposed to military actions, physical violence, or severe human suffering had higher levels of anxiety, depression, stress, and trauma-related symptoms. The war experience varied by gender, age, and living conditions. Participants who stayed in Ukraine had significantly lower anxiety, depression, stress, and trauma-related symptoms compared to those who moved abroad. Anxiety, depression, stress, low resilience, and subjective satisfaction with living conditions were predictors of trauma-related symptoms, including PTSD and CPTSD.

Conclusion

These findings suggest that the mental health of Ukrainians affected by the war was impacted differently depending on their level of exposure to violence and their living conditions. Additionally, the study identified several predictors of trauma-related symptoms, including PTSD and CPTSD, such as anxiety, depression, stress, low resilience, and subjective satisfaction with living conditions. Future research should further explore the relationships between trauma type, sociodemographic factors, resilience, stress, anxiety, depression, and PTSD and CPTSD to better understand the mediation mechanisms underlying these relationships and to develop effective interventions to support the well-being of Ukrainians during this difficult time.
The relations between socio-demographic information and negative symptoms, mental health, and quality of life: a latent profile analysis with psychotic patients in Kosovo
Authors: Uka Fitim , Konjufca Jon , Ramadani Fjolla , Arënliu Aliriza , Bërxulli Dashamir , Jovanović Nikolina , Russo Manuela
Published: July 2023

The current study aims to identify meaningful psychotic patients’ profiles by examining certain combinations of patient’s demographic and socio-economic variables (sex, age, marital status, number of children, cohabitant and level of education). Moreover, we aim to assess whether there is any significant effect of class membership (profile) on negative symptoms, health state, and quality of life among psychotic patients. A convenience sample of 103 patients (age: M = 22, SD = 1.75), was drawn from the clinical populations of Kosovo. Demographic and socio-economic data was obtained through individual interviews, meanwhile a battery of questionnaires was used to assess negative symptoms, mental health, and quality of life of patients. The 4-class solution was selected as the best fitting model and used in subsequent analyses. Results indicated a significant effect of class membership on health state, quality of life and negative symptoms. Practical implications are discussed.
Case report: Hyperactive delirium after a single dose of zolpidem administered additionally to psychopharmacotherapy including clozapine
Authors: Preiss Maximilian , Rabl Ulrich , Popper Valentin , Watzal Victoria , Treiber Michael , Ivkic Dominik , Praschak-Rieder Nicole , Naderi-Heiden Angela , Fugger Gernot , Frey Richard , Rujescu Dan , Bartova Lucie
Published: July 2023

The non-benzodiazepine hypnotic zolpidem is frequently administered as a short term psychopharmacotherapy for insomnia. Although it is well-established in a broad clinical routine and often well-tolerated, severe delirium and complex sleep behavior were reported in rare cases. Hereby, it remains unclear whether zolpidem's potential for delirium might be enhanced when combined with further psychopharmacotherapeutics. The present case report portrays a young male Caucasian inpatient with schizoaffective disorder, who was admitted due to severe hyperactive delirium after a single dose of zolpidem 10 mg that was administered in addition to already established psychopharmacotherapy including clozapine 200 mg/day, aripiprazole 15 mg/day and cariprazine 4.5 mg/day. In detail, disorientation, agitation, confabulations, bizarre behavior, and anterograde amnesia occurred shortly after ingestion of zolpidem and gained in intensity within a couple of hours. Once zolpidem was discontinued, the abovementioned symptoms subsided completely and did not reoccur. Since a clear temporal association could be drawn between the intake of zolpidem and the onset of hyperactive delirium, the present clinical experience should serve as a cautionary note for combining potent sedative-hypnotics and substances with anticholinergic properties, even in young adults in a good general condition. Moreover, our case argues for the necessity of further research into the pathomechanism of the interaction potential of non-benzodiazepines as zolpidem, especially with substances exerting anticholinergic properties, which are known for their potential to precipitate delirium. Therefore, the metabolic pathways of the concurrently administered substances should be further taken into account.
The effect of paired associative stimulation with a high-intensity cortical component and a high-frequency peripheral component on heart rate and heart rate variability in healthy subjects
Authors: Haakana P. , Holopainen K. , Nätkynmäki A. , Kirveskari E. , Tarvainen M. P. , Shulga A.
Published: July 2023

Objective

A novel protocol for paired associative stimulation (PAS), called high PAS, consists of high-intensity transcranial magnetic stimulation (TMS) and high-frequency peripheral nerve stimulation (PNS). High PAS was developed for spinal cord injury rehabilitation and targets plastic changes in stimulated pathways in the corticospinal tract, which improves motor function. As therapy interventions can last many weeks, it is important to fully understand the effects of high PAS, including its effect on the cardiovascular system. Heart rate variability (HRV) has been used to measure changes in both sympathetic and parasympathetic systems.

Methods

We used short-term HRV measurements to evaluate the effects of one 20-min session of high PAS on 17 healthy individuals. HRV was recorded for 5 min before (PRE), during (STIM), immediately after (POST), 30 min after (POST30), and 60 min after (POST60) the stimulation. Five participants repeated the HRV setup with sham stimulation.

Results

A significant decrease in low-frequency (LF) power (n.u.) (p = 0.002), low-frequency to high-frequency (HF) ratio (p = 0.017), in Poincaré plot [the standard deviation of RR intervals perpendicular to (SD1) and along (SD2) the line of identity SD2/SD1 ratio p < 0.001], and an increase in HF power (n.u.) (p = 0.002) were observed between PRE and STIM conditions; these changes were fully reversible immediately after stimulation. PRE to POST by 3% (p = 0.015) and continued to decline until POST60 by 5% (p = 0.011). LF power (ms2) (p = 0.017) and SD2 (p = 0.015) decreased from PRE to STIM and increased from PRE to POST (p = 0.025 and p = 0.017, respectively). The results from sham PAS exhibited a trend similar to active high-PAS stimulation.

Conclusions

High PAS does not have sustained effects during 60-min follow-up on cardiovascular functions, as measured by HRV. None of the short-term results indicates activation of the sympathetic nervous system in healthy individuals. Observed changes in HRV indicate higher parasympathetic activity during stimulation, which is reversible, and is plausibly explained by the fact that the participants spend 20 min without moving, talking, or using phones while being stimulated.
Complexity leadership in action: a team science case study
Authors: Jiang Gemma , Boghrat Diane , Grabmeier Jenny , Cross Jennifer E.
Published: July 2023

Introduction

This team science case study explores one cross-disciplinary science institute's change process for redesigning a weekly research coordination meeting. The narrative arc follows four stages of the adaptive process in complex adaptive systems: disequilibrium, amplification, emergence, and new order.

Methods

This case study takes an interpretative, participatory approach, where the objective is to understand the phenomena within the social context and deepen understanding of how the process unfolds over time and in context. Multiple data sources were collected and analyzed.

Results

A new adaptive order for the weekly research coordination meeting was established. The mechanism for the success of the change initiative was best explained by complexity leadership theory.

Discussion

Implications for team science practice include generating momentum for change, re-examining power dynamics, defining critical teaming professional roles, building multiple pathways towards team capacity development, and holding adaptive spaces. Promising areas for further exploration are also presented.