bluuebunny/crossref_metadata_2025 huggingface dataset:
A Proposal for the Sustainable Enhancement of the Cultural and Social Heritage of the Alps: The Project “La Montagna al Femminile”
Authors: Morazzoni Monica , Pecorelli Valeria , Di Napoli Matteo
Published: February 2025

The snow-based tourism, long a cornerstone of economic development for mountain destinations in the Italian Alps, is increasingly unsustainable. This study explores the potential of a cultural project dedicated to showcasing the representation of women in Alpine spaces as an alternative pull factor to alpine tourism. The project aims to revitalise the cultural and social heritage of Alpine regions, offering them renewed appeal while promoting sustainable development practices. Aligned with the Declaration of Jena, the project also aims to foster cultural sustainability by amplifying the often-overlooked contributions of women across professional, cultural, artistic, and recreational spheres in the Alps.
A System Coupling Analysis on the Integration of Scientific and Technological Innovation with Industrial Innovation for Sustainable Regional Development in China
Authors: Wang Huiyan , Liu Jianxu , Xu Yinliang
Published: February 2025

The integration of scientific and technological innovation with industrial innovation has emerged as a pivotal driver of sustainable economic growth. Utilizing the theory of system coupling, this study conceptualizes the integration process as complex and dynamic. It explores the interaction mechanisms between these innovations and establishes a systematic coupling evaluation index system. Additionally, methods such as the coupling coordination model and grey relational analysis are employed to quantitatively assess the integration levels across China and its 30 provincial regions from 2012 to 2022 in this study. Key factors influencing the enhancement of this integration are also identified. The findings indicate that, from 2012 to 2022, the coupling relationship between these two innovation systems has attained an advanced coupling state; however, the degree of coupling coordination remains at a primary level. Regionally, the coupling coordination degree exhibits features of unbalanced development: the eastern region reaches the highest level, albeit still at a barely coordinated stage, followed by the central region, which surpasses the northeastern region, while the western region exhibits the lowest degree. At the provincial level, notable discrepancies exist in the coupling coordination between these two innovation systems. Grey relational analysis reveals that scientific and technological input, along with industry–university–research collaboration, play particularly critical roles in enhancing the degree of integration.
Sustainability and Quality of Cultured Meat: Consumer Perceptions
Authors: Pasca Maria Giovina , Arcese Gabriella
Published: February 2025

Resource consumption, global greenhouse gas emissions, and their effects on human health have pushed the food sector to produce novel foods such as cultured meat. Cultured meat could respond to the demands for sustainable transformations in the food sector; however, are consumers ready to change their eating habits? This research analyses consumer perceptions of cultured meat by linking it to quality, health, sustainability, and socio-economic aspects. The study adopts a qualitative approach, and through in-depth interviews, explores Italian consumers’ perceptions of cultured meat. The findings show how cultured meat is perceived as a sustainable alternative that safeguards the environment, natural resources, and animal welfare. However, the research highlights the need for more information on the production phase of this novel food. Research into the hazards and risks of cultured meat is essential to confirm its safety. Indeed, further research and investments are needed to obtain information on the safety and reliability of this new food. The respondents feared introducing this new food as it could damage the actors involved in the agri-food chain by reducing jobs, and they were not inclined to abandon their culinary traditions. The results suggest to companies and governments which aspects to optimize and which factors to invest in to communicate with consumers.
Utilization of Non-Composted Human Hair Hydrolysate as a Natural and Nutrient-Rich Liquid Fertilizer for Sustainable Agro-Applications and Bio-Waste Management
Authors: Yetilmezsoy Kaan , Ilhan Fatih , Kıyan Emel
Published: February 2025

Human hair, commonly considered a discarded organic waste, is a keratin-rich material with remarkable potential for sustainable agriculture as an innovative resource. This study systematically explored the potential of non-composted human hair hydrolysates as eco-friendly and nutrient-rich liquid fertilizers, emphasizing their ability to enhance agricultural sustainability and mitigate organic waste accumulation. Eight distinct hydrolysates prepared with alkaline solutions were evaluated for their effects on plant growth using red-hot chili pepper (Capsicum frutescens) as the primary model under greenhouse conditions. The present study introduces a novel approach by employing an advanced digital image analysis technique to quantitatively assess 37 distinct plant growth parameters, providing an unprecedented depth of understanding regarding the impact of liquid human hair hydrolysates on plant development. Additionally, the integration of pilot-scale field trials and multi-species evaluations highlights the broader applicability and scalability of these hydrolysates as sustainable fertilizers. Collectively, these features establish this research as a pioneering contribution to sustainable agriculture and bio-waste management. The top-performing hydrolysates (KCaMgN, KMgN, KCaN) demonstrated significant enhancements in plant growth metrics, with fresh weight reaching up to 3210 mg, projected leaf area of approximately 132 cm2, and crown diameter of 20.91 cm for the best-performing formulations, outperforming a commercial organomineral fertilizer by 20–46% in overall growth performance. Furthermore, observational studies on various species (such as bird of paradise flower (Strelitzia reginae), avocado (Persea americana), lemon (Citrus limon L.), Mazafati date (Phoenix dactylifera L.), and red mini conical hot pepper (Capsicum annuum var. conoides) and field trials on long sweet green peppers (Capsicum annuum) confirmed the broad applicability of these hydrolysates. Toxicity assessments using shortfin molly fish (Poecilia sphenops) validated the environmental safety of plants cultivated with hydrolysates. These findings highlight that human hair hydrolysates offer a sustainable alternative to synthetic fertilizers, contributing to waste management efforts while enhancing agricultural productivity.
Rule Inferring for Engineering Quality Risk Management Based on Ontology in Housing Construction
Authors: Jiang Siyang , Cao Xinying
Published: February 2025

To address the challenges surrounding information sharing and low efficiency during the engineering quality risk management process, this paper constructs a digital process for engineering quality risk management. Engineering quality risk factors were identified through literature analysis and synthesis, and relevant standards, specifications, and project information were collected to construct an engineering quality risk information ontology. The Semantic Web Rule Language (SWRL) was used to implement rule-based rapid identification of risk factors, enabling stakeholders to query information in real-time and perform dynamic information updates promptly. To validate the effectiveness of ontology-based rule inferring for engineering quality risk management, a case study on a project in Guangzhou demonstrated that the proposed rule-inferring effectively identified risk factors and significantly reduced engineering quality risks. The ontology-based digital workflow optimized the engineering quality management workflow and contributed to more efficient and robust risk management practices. The findings provide a meaningful reference for advancing engineering quality risk management methods.
How Does Corporate Environmental Performance Contribute to Firm Performance and Customer Satisfaction? A Longitudinal Investigation
Authors: Alyahya Mansour , Agag Gomaa
Published: February 2025

This research adopted a distinctive approach to explore the link between corporate environmental performance, customer satisfaction, and firm performance. It also examines the moderating role of digital transformation on these relationships. We collected longitudinal data covering 2745 firm-year observations from the U.S. Standard & Poor’s (S&P) 500. Our study utilised “the generalised method of moments (GMM) technique” to analyse the longitudinal data. The results revealed that a one-unit enhancement in CEP results in, on average, a 10.1% rise in the growth rate of ROA, a 13.40% increase in Tobin’s Q, and a 14.2% increase in customer satisfaction. Moreover, digital transformation moderates the links between CEP, firm performance, and customer satisfaction. The findings of our study guide policymakers, researchers, shareholders, and managers in addressing the challenge of corporate environmental performance.
Presenteeism and Sustainable Occupational Health in the Workplace
Authors: Yang Tianan , Deng Wenhao , Liu Ran , Deng Jianwei
Published: February 2025

In the face of the profound impacts of global digitization, aging, and major public health emergencies on work patterns, occupational health in the workplace is of inescapable importance for the achievement of the Sustainable Development Goals [...]
Analysis of Airflow Organization in Buses Air-Conditioned by Direct Evaporative Coolers
Authors: Zhou Wenhe , Liu Mengdie , Duan Lin
Published: February 2025

Considering the energy-saving advantages of the direct evaporative cooler (DEC) compared to the traditional air conditioning system (TAC), this study aims to indicate its ability to improve the thermal comfort and the indoor air quality of the bus compared to the bus air-conditioned by the traditional compressor system. Taking a bus in Lanzhou as the object, the numerical model and method were first verified by an experimental method. Then, numerical analyses were simultaneously carried out in both bus models, which were air-conditioned by TAC and DEC, respectively. The results showed that the thermal comfort of the bus air-conditioned by DEC is more satisfactory, and the indoor air quality is better. Additionally, the bus air-conditioned by DEC achieves a 43.7% improvement in the temperature efficiency and a 31.3% improvement in the ventilation efficiency compared to the bus air-conditioned by TAC. The conclusion will provide valuable insights into the application of DEC in buses in dry regions.
The Impact of Green Finance Policies on Corporate Debt Default Risk—Evidence from China
Authors: Fan Li , Xu Weidong
Published: February 2025

As global climate change issues have become increasingly severe, green finance has gained widespread attention from governments and financial institutions as a crucial tool for promoting sustainable development. This paper explores the impact of green finance reform pilot zones on corporate debt default risks based on a difference-in-differences model. We found that green finance policies significantly increase corporate debt default risks by exacerbating financing constraints and reducing stock liquidity. A heterogeneity analysis revealed that polluting enterprises, non-state-owned enterprises, and companies in the Eastern region are more susceptible to the impacts of this policy. This paper suggests that the government should formulate differentiated green finance policies tailored to different types of enterprises and regional characteristics.
Evaluating Spatial Attributes of Surface Colors Under Daylight and Electrical Lighting in Sustainable Architecture
Authors: Espinoza-Sanhueza Carolina , Hébert Marc , Lalonde Jean-François , Demers Claude
Published: February 2025

This paper investigates the spatial attributes of the color properties and brightness characteristics of sustainable architectural strategies including daylight, electrical lighting, and surface color in architecture, which could potentially impact users’ spatial experiences. Images of 48 spaces varying in surface color configurations, type of light source, and position of the lighting strategy were evaluated. The analyses included assessments of color palettes, descriptors based on saturation and brightness properties, and brightness distribution maps. The results indicate that lighting design and types of light source influence the saturation and brightness properties of the perceived hues evaluated in the same environment, leading to variations in color descriptors or adjectives. Furthermore, this study demonstrates that variations in brightness between bright and dark zones, the creation of focal points, and perceived spatial fragmentation depend on the reflectance of the colors applied in the surfaces, the position of the lighting, and the type of light source. This study does not aim to establish best practices for enhancing users’ emotions through architecture. Instead, it explores how variations in color and light influence perceptual descriptions that have been previously associated with emotional responses. This research recognizes the impact of sustainable strategies including surface colors under daylight and electrical lighting on users’ spatial experiences.
Coal Mines and Multi-Faceted Risks in the United States: On a Path Toward a Sustainable Future or Emptying Out?
Authors: Greenberg Michael R. , Schneider Dona
Published: February 2025

Coal is a major source of fuel in many countries, but its mining and use increase the impacts on human and environmental health. Given the wide variation in coal use by nation, we focused on one—the United States. Specifically, we examined the public health, community, and demographic characteristics of 130 U.S. counties with underground and/or surface coal mines that exemplify a land use that leads to a combination of natural and anthropogenic risks. We compared these 130 to other U.S. counties, finding that the coal counties had poorer health outcomes and behaviors, fewer community assets, lower socioeconomic status, and higher population losses compared to the remaining counties in their host states and other U.S. counties. Next, we looked for differences within the 130 coal counties. Counties with the most coal mines and production had the worst outcomes, especially when located in rural areas. We then examined federal programs to assist these coal communities, observing that the largest federal government programs appear to be sending more resources to the most distressed coal counties compared to the less distressed ones. The daunting challenge for the most heavily coal-dependent counties, their state governments, and federal government supporters is to jointly develop plans that are realistic and affordable, take advantage of local natural and human resources, and offer a path to sustainable existence. If the efforts do not succeed, these places run the risk of becoming politically invisible and their populations are likely to empty out.
An LCA Study of Various Office Building Shapes Focusing on Operational Energy—A Case of Hamburg
Authors: Shokouhi Samira , Weidlich Ingo
Published: February 2025

The design and configuration of buildings can play a major role in influencing the environmental impacts of the built environment. This paper explores the relation of building shape and it’s environmental impacts by employing a life cycle assessment (LCA) framework. The primary objective is to contribute to the ongoing discourse on sustainable construction practices by exploring alternatives in office building shapes and heights. The initial focus of our study centers on a set of plan shapes based on different combinations of a 12 × 14 square meter modular unit. This set introduces variations with and without courtyards, coupled with three distinct heights of 3, 6, and 12 m (1, 2, and 4 stories). Expanding our exploration, we introduce a second set of standard geometric shapes, namely square, pentagon, hexagon, heptagon, octagon, and circle. We assess the annual energy demand of buildings with these plan shapes and conduct an LCA analysis focused on the operational energy use stage in the eLCA tool to quantify their environmental implications, focusing on global warming potential (GWP) and primary energy non-renewable total (PENRT) indicators. Through calculations and comparisons of the LCA results, this paper provides insights into the environmental trade-offs and benefits associated with different building plan shapes and heights.
Exploring the Roles of Work Engagement, Psychological Empowerment, and Perceived Organizational Support in Innovative Work Behavior: A Latent Class Analysis for Sustainable Organizational Practices
Authors: Ergun Ercan , Tunca Sezai , Cetinkaya Gulsum , Balcıoğlu Yavuz Selim
Published: February 2025

This study examines the relationship among work engagement, psychological empowerment, and perceived organizational support in fostering innovative work behavior (IWB) within sustainability-oriented organizations. Using latent class analysis (LCA), employees were classified into distinct profiles based on their levels of engagement, empowerment, and perceived support. The findings indicate that employees with higher engagement and organizational support exhibit significantly greater innovative work behavior, while those with lower psychological and organizational resources face challenges in contributing to innovation. This study also reveals that sustainability practices enhance the positive impact of these resources on innovation, improving both employee well-being and organizational performance. Grounded in Structural–Interactionist Innovation Theory, Self-Efficacy Theory, and Social Exchange Theory, this research provides practical insights for organizations to develop targeted strategies, such as tailored training programs and leadership support, to promote innovation and sustainability.
Digital Transformation for Sustainability in Industry 4.0: Alleviating the Corporate Digital Divide and Enhancing Supply Chain Collaboration
Authors: Li Qi , Tian Weijian , Zhang Hua
Published: February 2025

The swift advancement of digital technologies under Industry 4.0 has significantly transformed business operations and supply chain management. These advancements hold the potential to improve efficiency, reduce waste, and foster sustainable development; however, they also create challenges due to the uneven adoption of digital technologies across enterprises. (1) Background: The adoption of digital technologies across supply chains is uneven, resulting in a digital divide between enterprises. This disparity disrupts supply chain collaboration and alignment with sustainable practices. (2) Methods: This research examines how the corporate digital divide affects the supply–demand imbalance by employing a quantitative method to identify obstacles and strategies for improving collaboration. This research employs a quantitative approach, specifically multiple regression analysis, to investigate how the digital divide among enterprises affects the supply–demand imbalance and to identify strategies for overcoming collaboration barriers. The research utilizes firm-level data from the Chinese stock market and accounting research databases and performs robustness checks, including methods such as the instrumental variable approach and the Heckman two-stage model, to ensure the validity of the findings. (3) Results: The study finds that the corporate digital divide exacerbates imbalances in both upstream and downstream chains. Elevating supply chain resilience has effectively alleviated this relationship. Specifically, the strengthening of resource resilience and process resilience has effectively alleviated the impact of the corporate digital divide on the supply–demand imbalance in the upstream supply chain, while the enhancement of system resilience and product resilience has effectively mitigated the impact of the corporate digital divide on the supply–demand imbalance in the downstream supply chain. Heterogeneity analysis indicates that the impact of the digital divide in supply chain enterprises on supply–demand imbalance varies under different conditions of network centrality, supply chain concentration, government digital focus, and enterprise nature. (4) Conclusions: To foster sustainability in Industry 4.0, enterprises must bridge the corporate digital divide and enhance supply chain collaboration. It is recommended to mitigate upstream supply chain disruptions caused by the digital divide by improving resource and process resilience while alleviating downstream impacts through strengthened system and product resilience. Furthermore, fostering collaborative digital development among enterprises is essential for optimizing supply chain sustainability.
Monitoring of Pathogens Carried by Imported Flies and Cockroaches at Shenzhen Ports
Authors: Zhang Siqi , Zhao Chunzhong , Liu Guoping , Guo Liwei , Zhang Ran , Yan Junyu , He Jianan , Guo Cheng
Published: February 2025

This study tested the efficacy of xenomonitoring using contaminated flies and cockroaches at ports in Shenzhen by analysing sample data from imported flies and cockroaches from October 2023 to April 2024 to identify the pathogens they carried. Among all the samples of flies and cockroaches collected, Musca domestica vicina and Blattella germanica accounted for the highest proportion, 27.59% and 66.47%, respectively. Their positive rates for carrying Staphylococcus aureus were also the most significant, reaching 4.35% and 6.47%, respectively. The imported flies and cockroaches mainly came from Asia, with the highest proportion coming from Hong Kong, at 97.71% and 92.11%, respectively. Metagenomic sequencing indicated that the pathogens carried by the flies and cockroaches from different regions of Asia were generally similar but showed some differences. Flies from Southeast Asia, East Asia, South Asia, and West Asia and cockroaches from Southeast Asia, East Asia, and West Asia harboured unique opportunistic pathogens capable of causing gastrointestinal and respiratory infections in humans. Specifically, flies carried pathogens such as Campylobacter jejuni, Bacillus anthracis, Bacteroides fragilis, and Bordetella bronchiseptica, while cockroaches carried B. fragilis, Clostridium tetani, and Bacillus cereus. Our findings provide data support for future risk assessments of pathogens carried by imported vectors.
Efficacy of Different Combinations of Direct-Acting Antivirals Against Different Hepatitis C Virus-Infected Population Groups: An Experience in Tertiary Care Hospitals in West Bengal, India
Authors: Bakshi Sagnik , Chattopadhyay Partha , Ahammed Mahiuddin , Das Raina , Majumdar Moumita , Dutta Supradip , Nath Shreyasi , Ghosh Anwesha , Bhattacharjee Uttaran , Baskey Upasana , Sadhukhan Provash Chandra
Published: February 2025

Hepatitis C virus (HCV) is a global public health problem, but advancements in HCV treatment have improved the cure rate. This study evaluated the effectiveness of direct-acting antivirals (DAAs) in HCV-infected patients from May 2021 to April 2023 in collaboration with tertiary care hospitals in West Bengal. The HCV viral load was monitored via qRT-PCR. Sanger sequencing was performed to determine the HCV genotypes. The clinicians prescribed the patient treatment regime. The maximum number of patients in the study population (N = 398) were compensated cirrhosis patients (46.28%). The overall SVR rate of the study population was 94.47%. The decompensated cirrhosis patients experienced the lowest SVR rate (88.89%). The maximum number of patients were prescribed sofosbuvir/daclatasvir (63.77%), and the lowest SVR rate (93.23%) was observed with this treatment regime. In the study population, GT-3 was the predominant (67.43%) circulating genotype, followed by GT-1 and -4. Among 398 patients, 22 (5.53%) were non-responsive to DAA treatment. Out of these 22 non-responder patients, 77.27% (n = 17) were GT-3-infected (3a:10; 3b:07), followed by GT-1 (1c: 04; 1b: 01). Thus, increasing numbers of DAA non-responsive cases among HCV GT-3-infected and decompensated cirrhosis patients may pose serious threats in the future.
Heterologous Immunization with Improved HIV-1 Subtype C Vaccines Elicit Autologous Tier 2 Neutralizing Antibodies with Rapid Viral Replication Control After SHIV Challenge
Authors: Chege Gerald K. , Chapman Rosamund E. , Keyser Alana T. , Adams Craig H. , Benn Kealan , van Diepen Michiel T. , Douglass Nicola , Lambson Bronwen , Hermanus Tandile , Moore Penny L. , Williamson Anna-Lise
Published: February 2025

We previously reported on HIV vaccines that elicited autologous Tier 2 neutralizing antibodies (nAbs) in rabbits. In the current study, we sought to establish a proof of concept that HIV vaccines using identical designs elicit Tier 2 nAbs in arhesus macaque (RM) model. DNA and MVA vaccines expressing SIV Gag and HIV-1 Env antigens were constructed, and in vitro expression was confirmed. A soluble envelope protein (gp140 Env) was expressed from a stable HEK293 cell line and purified using lectin affinity and size exclusion chromatography. The expression and secretion of SIV Gag and HIV-1 Env by the DNA and MVA vaccines was verified in vitro. Five RMs were inoculated with two DNA, followed by two MVA, and finally with two gp140 Env vaccines at weeks 0, 4, 8, 12, 20 and 28. Vaccine-induced T cell immunity was measured by IFN-γ ELISpot while nAbs were evaluated against MW965 (Tier 1A), 6644 (Tier 1B), autologous ZM109.5A and a closely-related ZM109.B4 (Tier 2) pseudovirions. Vaccinated RMs were challenged intrarectally with simian-human immunodeficiency virus (SHIV), four weeks after the final vaccination, as was an unvaccinated control group (n = 4). Following vaccination, all the animals developed moderate IFN-γ ELISpot responses after the DNA vaccinations which were boosted by the MVA vaccine. After the gp140 Env boost, all animals developed nAbs with peak median titres at 762 (MW965) and 263 (ZM109.5A). The vaccinated animals became infected after a similar number of challenges to the unvaccinated controls, and the resultant number of viral copies in the blood and the lymphoid tissues were similar. However, the duration of detectable viraemia in the vaccinated animals (median: 2 weeks) was shorter than the controls (median: 8.5 weeks). These data show that the vaccines elicited robust cellular and functional humoral immune responses that resulted in a quicker control of viraemia.
Sex and Age-Based Differences in Immune Responses to a Peptide Vaccine for Melanoma in Two Clinical Trials
Authors: Vilasi Serena M. , Slingluff Craig L.
Published: February 2025

Objectives: Little is known about the impact of patient age and biological sex on immune responses to melanoma vaccines, especially CD4+ T cell immune responses to peptides presented by Class II MHC molecules. Methods: We assessed the impact of age and sex on CD4+ T cell and antibody responses to a mixture of six melanoma helper peptides (6MHP) and on CD8+ T cell responses when vaccinating with 12 class I MHC-restricted melanoma peptides (12MP) plus either 6MHP or a tetanus helper T cell peptide (Tet). We hypothesized that immune responses would be greater in men and in younger patients. Results: We found differences in immune response by sex, but they favored female patients and were only evident for helper T cell responses to Tet with a weak trend to higher T cell responses to 12MP in female patients vaccinated with 12MP + Tet. The age-based differences favored younger patients but only for immune response to 12MP when inoculated with 12MP + Tet. Conclusions: These findings reinforce the importance of assessing sex- and age-based differences in immune responses to cancer vaccines and other immune therapies. There is also a need to understand the reasons for such differences.
Amonabactin Synthetase G Regulates Aeromonas hydrophila Pathogenicity Through Modulation of Host Wnt/β-catenin Signaling
Authors: Tang Yiyang , Liu Xiaofeng , Zeng Chuyi , Liu Yujun , Yang Ye , Hu Jiayi , Li Pingyuan , Zhou Zejun
Published: February 2025

Background/Objectives: Aeromonas hydrophila is a significant opportunistic pathogen with a broad host range. It produces a catecholate siderophore, amonabactin, during iron starvation, but the in vivo infection mechanism that involves amonabactin is unclear. This study aims to elucidate the role of amonabactin synthetase G (AmoG) in the pathogenicity of A. hydrophila and its impact on gut barrier function. Methods: ΔAmoG was generated by deleting the AMP-binding domain of AmoG in A. hydrophila CCL1. In vivo infection experiments were conducted to assess the mutant’s iron-chelating ability and pathogenicity. Complementation of ΔAmoG with AmoG (ΔAmoG-C) was performed to confirm the observed phenotypes. Transcriptomic and qRT-PCR analyses were used to investigate gene expression changes in infected fish. Goblet cell counts, tight junction expression, and D-lactic acid and LPS levels were measured to evaluate gut barrier function. Results: ΔAmoG exhibited impaired iron-chelating ability and reduced pathogenicity compared to wild-type CCL1. Complementation with AmoG restored virulence in ΔAmoG-C. Transcriptomic and qRT-PCR analyses revealed an elevated expression of Wnt/β-catenin pathway components and antimicrobial genes in ΔAmoG-infected fish. Further investigation indicated increased goblet cells and an enhanced expression of tight junctions, as well as lower D-lactic acid and LPS levels, in ΔAmoG-infected fish. However, gut permeability, bacterial load, and lethality did not significantly differ between CCL1, ΔAmoG, and ΔAmoG-C infections when the Wnt/β-catenin pathway was activated. Conclusions: AmoG plays a crucial role in A. hydrophila pathogenicity by modulating host Wnt/β-catenin signaling and gut mucosal barrier function. This study provides insights into the pathogenesis of A. hydrophila and potential therapeutic targets.
Novel sACE2-Anti-CD16VHH Fusion Protein Surreptitiously Inhibits SARS-CoV-2 Variant Spike Proteins and Macrophage Cytokines, and Activates Natural Killer Cell Cytotoxicity
Authors: Sheikhi Abdolkarim , Baghaie Leili , Rahbarizadeh Fatemeh , Safarzadeh Kozani Pooria , Moradian Cobra , Davidi Mohammadreza , Baharifar Narges , Kaboli Golnaz , Sheikhi Mehdi , Li Yunfan , Meghdadi Mohammadamin , Yaish Abdulrahman M. , Yu Aiden H. , Harless William W. , Szewczuk Myron R.
Published: February 2025

Background/Objectives: The SARS-CoV-2’s high mutations and replication rates contribute to its high infectivity and resistance to current vaccinations and treatments. The primary cause of resistance to most current treatments aligns within the coding regions for the spike S protein of SARS-CoV-2 that has mutated. As a potential novel immunotherapy, we generated a novel fusion protein composed of a soluble ACE2 (sACE2) linked to llama-derived anti-CD16 that targets different variants of spike proteins and enhances natural killer cells to target infected cells. Methods: Here, we generated a novel sACE2-AntiCD16VHH fusion protein using a Gly4Ser linker, synthesized and cloned into the pLVX-EF1alpha-IRES-Puro vector, and further expressed in ExpiCHO-S cells and purified using Ni+NTA chromatography. Results: The fusion protein significantly blocked SARS-CoV-2 alpha, beta, delta, gamma, and omicron S-proteins binding and activating angiotensin-converting enzyme receptor-2 (ACE2) on ACE2-expressing RAW-Blue macrophage cells and the secretion of several key inflammatory cytokines, G-CSF, MIP-1A, and MCP-1, implicated in the cytokine release storm (CRS). The sACE2-Anti-CD16VHH fusion protein also bridged NK cells to ACE2-expressing human lung carcinoma A549 cells and significantly activated NK-dependent cytotoxicity. Conclusions: The findings show that a VHH directed against CD16 could be an excellent candidate to be linked to soluble ACE2 to generate a bi-specific molecule (sACE2-AntiCD16VHH) suitable for bridging effector cells and infected target cells to inhibit SARS-CoV-2 variant spike proteins binding to the ACE2 receptor in the RAW-Blue cell line and pro-inflammatory cytokines and to activate natural killer cell cytotoxicity.
Research on the Dynamic Response Characteristics of a Railway Vehicle Under Curved Braking Conditions
Authors: Zhao Chunguang , Fan Zhiyong , Li Peixuan , Gebreyohanes Micheale Yihdego , Wang Zhiwei , Mo Jiliang
Published: February 2025

When a railway train runs along a curved track with braking, the dynamic behaviors of the vehicle are extremely complex and difficult to accurately reveal due to the coupling effects between the wheel–rail interactions and the disc–pad frictions. Therefore, a rigid–flexible coupled trailer car dynamics model of a railway train is established. In this model, the brake systems and vehicle system are dynamically coupled via the frictions within the braking interface, wheel–rail relationships and suspension systems. Furthermore, the effectiveness of the established model is validated by a comparison with the field test data. Based on this, the dynamic response characteristics of vehicle under curve and straight braking conditions are analyzed and compared, and the influence of the curve geometric parameters on vehicle vibration and operation safety is explored. The results show that braking on a curve track directly affects the vibration characteristics of the vehicle and reduces its operation safety. When the vehicle is braking on a curve track, the lateral vibration of the bogie frame significantly increases compared to the vehicle braking on a straight track, and the vibration intensifies as the curve radius decreases. When the curved track maintains equilibrium superelevation, the differences in primary suspension force, wheel–rail vertical force, and wheel axle lateral force between the inner and outer sides of the first and second wheelsets are relatively minor under both straight and curved braking conditions. Additionally, under these circumstances, the derailment coefficient is minimized. However, when the curve radius is 7000 m, with a superelevation of 40 mm, the maximum dynamic wheel load reduction rate of the inner wheel of the second wheelset is 0.54, which reaches 90% of the allowable limit value of 0.6 for the safety index, and impacts the vehicle running safety. Therefore, it is necessary to focus on the operation safety of railway trains when braking on curved tracks.
Development of a Multiplex Conventional PCR Assay for Concurrent Detection of FAdV-4, FAdV-8b, and FAdV-11
Authors: Kang Su-kyung , Park Dam-Hee , Min Kyeongcheol , Yoo Sung-Sik , Yoon In-Joong , Mo Jongseo
Published: February 2025

Fowl adenovirus (FAdV) belongs to the Aviadenovirus genus within the Adenoviridae family. FAdVs are widely distributed and associated with various diseases in poultry, including adenoviral gizzard erosion (AGE), hepatitis-hydropericardium syndrome (HHS), and inclusion body hepatitis (IBH). In this study, we developed a multiplex conventional PCR for simultaneously detecting FAdV-4, -8b, and -11 by targeting the hexon gene. The multiplex PCR was optimized for primer concentrations and thermocycling conditions. The optimal primer concentration combination was set at 0.125 μM for FAdV-4, -8b, and 0.25 μM for FAdV-11. Under these conditions, the limit of detection (LOD) was 103 copies/μL of plasmid standards for FAdV-4, -8b, and -11. These results demonstrated that the developed multiplex PCR method exhibits high specificity and sensitivity, with no observed cross-reactivity among these serotypes or with other poultry viruses. Therefore, this multiplex PCR will be an effective tool for accurate serotyping of FAdV-4, -8b, and -11, enabling more precise identification and differentiation of these three serotypes.
Study on Shear Creep Characteristics of Carbonaceous Mud Shale at Different Moisture Contents Under Temperature Cycling
Authors: Xue Fei , Hu Bin , Zhong Zhuoxi , Zhang Hongjun , Li Haiying
Published: February 2025

Mine slope landslides can lead to significant geological disasters. To investigate the impact of temperature cycling on the internal mechanisms that trigger these disasters in weak interlayers with varying moisture contents, a THMC-B multi-field coupled simulation device was employed to conduct shear creep tests on carbonaceous mud shale with varying moisture contents across 16 temperature cycles (ranging from −5 °C to 65 °C). Based on the observed creep characteristics and related patterns, a rheological constitutive model for carbonaceous mud shale was established to characterize the damage effects at different moisture contents during temperature cycling. The experimental results indicate the following: under temperature cycling conditions, an increase in moisture content rapidly reduces the mechanical properties of carbonaceous mud shale, rendering it more susceptible to shearing at the same failure stress level and consequently shortening the overall creep time; higher moisture content prolongs the duration of the deceleration creep stage in carbonaceous mud shale; and the improved constitutive model accurately represents the entire shear creep process of carbonaceous mud shale, with fitting coefficients exceeding 0.95. These research findings can provide certain references and insights for the study of shear creep characteristics of weak interlayers in mine slopes.
Data-Driven Modeling of Electric Vehicle Charging Sessions Based on Machine Learning Techniques
Authors: Kene Raymond O. , Olwal Thomas O.
Published: February 2025

The increased demand for electricity is inevitable due to transport sector electrification. A major part of this demand is from electric vehicle (EV) charging on a large scale, which is now a growing concern for the grid power distribution system. The lack of insight into grid energy demand by EVs makes it difficult to manage these consumptions on a large scale. For any grid load management application to be effective in minimizing the impact of uncontrolled charging, there is a need to gain insight into EV energy demand. To address this issue, this study presents data-driven modeling of EV charging sessions based on machine learning (ML) techniques. The purpose of using ML as an approach is to provide insight for estimating future energy demand and minimizing the impact of EV charging on the grid. To achieve the aim of this study, firstly, we investigated the impact of large-scale charging of EVs on the grid. Based on this, we formulated an objective function, expressed as a sum of utility functions when EVs charge on the grid with constraints imposed on voltage levels and charging power. Secondly, we employed a graphical modeling approach to study the temporal distribution of EV energy consumption based on real-world datasets from EV charging sessions. Thirdly, using ML regression models, we predicted EV energy consumption using four different models of fine tree, linear regression, linear SVM (support vector machine), and neural network. We used 5-fold cross-validation to protect against overfitting and evaluated the performances of these models using regression analysis metrics. The results from our predictions showed better accuracy when compared with the results from the work of other authors.
Estratégias de desinformação contra o programa Bolsa Família
Authors: Morais Lana Krisna de Carvalho , Rocha Heitor Costa Lima da
Published: February 2025

A pesquisa analisa desinformações disseminadas contra o programa social de distribuição de renda, Bolsa Família, a partir de recortes de falas oficiais, reciclagem de notícias antigas, manipulação dos fatos, entre outras táticas. Tem-se como problema norteador do estudo a questão: como funciona o processo de desinformação sobre o programa Bolsa Família? Objetiva-se compreender as estratégias de desinformação contra o programa. De forma específica, classificar os procedimentos manipulatórios a partir do recorte analisado; identificar as técnicas afetivas e cognitivas; e problematizar sobre o impacto social do processo de desinformação. Para tanto, procede-se à feitura do artigo caminhos metodológicos que perpassam pelo estudo de caso, a partir da seleção de três desinformações checadas entre janeiro e março de 2023. O recorte temporal em questão foi escolhido por se tratar do período de preparação para retomada da política de transferência de renda, criada em 2003 e alterada ao longo da gestão de Jair Bolsonaro para dar lugar ao Auxílio Emergencial. A pesquisa fundamenta-se nos conceitos de Philippe Breton (1999), UNESCO (2019) e Abramo (2003). Desse modo, observa-se que as desinformações sobre o programa Bolsa Família fizeram o uso de conexão falsa, conteúdo enganoso e conteúdo fabricado, de técnicas manipulatórias como convencimento a partir do afeto e da estética, o que permite concluir que a prática de desinformar opera como um mecanismo de manipulação que fomenta discursos xenofóbicos e de ódio.
Análise do filme "Holocausto brasileiro" sob a perspectiva da psicologia social
Authors: Veras Vitória Barbosa , Brandão Ana Beatriz de Araújo , Silva Hertha Monteiro da
Published: February 2025

A reforma psiquiátrica no Brasil representou um marco na luta pela humanização do cuidado em saúde mental, buscando romper com um modelo manicomial que, por décadas, isolou e marginalizou pessoas com transtornos mentais. Este estudo analisa o documentário Holocausto Brasileiro sob a perspectiva da psicologia social, adotando uma abordagem qualitativa. A análise evidencia cenas e relatos de violência física e negligência nos manicômios, revelando as condições desumanas a que os pacientes eram submetidos no Hospital Colônia de Barbacena. Com base nas reflexões de Martín-Baró sobre violência estrutural, alimentada pelo sistema capitalista, o estudo argumenta que superar a lógica manicomial exige mais do que o fechamento de instituições. É fundamental a implementação de políticas públicas eficazes e a promoção de um cuidado inclusivo e humanizado para pessoas em sofrimento psíquico.
O uso da Inteligência Artificial no tratamento de pacientes com danos neurológicos severos: uma revisão integrativa de literatura
Authors: Damasceno Evelyn Ravena Rodrigues
Published: February 2025

O artigo aborda o uso da inteligência artificial (IA) no tratamento de pacientes com danos neurológicos, com ênfase em diagnósticos mais precisos e tratamentos personalizadas par condições complexas. O objetivo é explorar como a IA contribui para aprimoramento o atendimento neurológico, desde o diagnostico até o acompanhamento de doenças como Alzheimer e Parkinson. A metodologia envolve uma revisão integrativa de literatura como seleção de artigos de 2019 a 2024 em base de dados como Scielo, PubMed e BVS, focando no impacto da IA na neurologia. No desenvolvimento, o estudo analisa os avanços  da IA em identificar padrões complexos em dados clínicos e de imagem, apoiando diagnósticos mais rápidos e detalhados. Os resultados indicam que a IA auxilia médicos na interpretação de exames, permitindo tratamentos mais eficazes e personalizados. A pesquisa destaca ainda a relevância de ampliar estudos para integrar dados de variadas fontes e desenvolver tecnologias de IA acessíveis, aumentando seu uso em contextos com menos recursos.
Medicações antidiabéticas e a busca por emagrecimento: uma revisão da literatura
Authors: Tfaile Rafael Longhini , Leonel Caio Henrique Alves , Leite Eduarda Borges , Menezes Camila Baldi , Manfio Victor Jose , Fernandes Josefa Maria Dias da Silva
Published: February 2025

Este artigo propõe-se a realizar uma revisão detalhada sobre a crescente tendência de uso de medicamentos, inicialmente destinados ao tratamento do diabetes mellitus, por indivíduos que buscam um método rápido e eficaz de emagrecimento. Nos últimos anos, tais medicamentos têm ganhado popularidade entre pessoas que desejam perder peso. Essa prática tem se tornado comum, especialmente entre pessoas que não conseguem obter os resultados desejados por meio de dietas e exercícios convencionais. No entanto, é crucial que esse uso seja monitorado por profissionais de saúde para evitar possíveis efeitos adversos e garantir a segurança dos pacientes. A fim de alcançar o objetivo deste trabalho, foi realizada uma revisão de literatura, nos seguintes bancos de dados: U. S. National Library of Medicine (PubMed) e Scientific Electronic Library Online (SciELO). Foram utilizadas palavras como obesidade, diabetes mellitus e semaglutida para a busca.
Tratamento clínico da angiodisplasia intestinal: relato de caso
Authors: Custódio Natalia Carneiro Canedo , Medeiros Larissa Leandro , Neto Álvaro Modesto da Silva Rodrigues
Published: February 2025

A angiodisplasia intestinal é uma malformação vascular sem motivo aparente, mas cuja fisiopatologia pode envolver o afilamento do endotélio vascular e da camada muscular da parede intestinal. Isso pode ocasionar uma ruptura, e consequentemente um sangramento. A apresentação clínica pode se dar por sangramento digestivo ou anemia, demandando diagnóstico precoce. O relato de caso exposto trata de uma paciente idosa com episódios importantes de melena, necessitando de hemotransfusões. Foram realizadas endoscopias, colonoscopias, angiotomografias e cápsula endoscópica para o diagnóstico. Após algumas abordagens terapêuticas o caso foi controlado por meio de tratamento medicamentoso visto que o alto risco cardiovascular contraindicava cirurgia. O uso de octreotide foi efetivo no controle do sangramento, sem necessidade de novas hemotransfusões.
Mucormicose de seios paranasais e órbita em paciente diabético: relato de caso
Authors: Lavoyer Lorena Moreira , Sanson Nicole Zanzarini , Damasceno Hágda Pires Moreira , Cunha Pedro Henrique Borges da , Fuga Marília Prior , Nose Juliana Teresa , Molina Rodrigo Juliano
Published: February 2025

RESUMOA mucormicose é uma infecção fúngica grave, frequentemente associada ao diabetes mellitus descontrolado. Relatamos o caso de um homem de 65 anos, diabético não tratado, que apresentou cefaleia intensa, ptose palpebral, amaurose e secreção nasal purulenta. Na admissão, apresentava grande descompensação do diabetes mellitus. Após antibioticoterapia inicial ineficaz, uma biópsia confirmou a presença de hifas compatíveis com Mucorales. A ressonância revelou destruição óssea e infecção extensa nos seios paranasais e órbita esquerda. Tratado com anfotericina B lipossomal, apresentou melhora parcial, mas com prognóstico reservado para o olho afetado. O caso destaca a importância do diagnóstico precoce, controle glicêmico rigoroso e manejo antifúngico agressivo para melhores desfechos.
Incidência da mastoidite relacionado a otite média aguda em lactentes
Authors: Vainchelboim Rachel Soares , Granito Juliana Ganz , Wong Louisa Ral Yen , Fernandes Lia Reis , Garcia Ligia Costa Genova , Sanches Tatiana Araújo Cócaro
Published: February 2025

Considerando-se a suscetibilidade dos lactentes à otite média e a possibilidade de evolução desta para mastoidite, foi realizada a revisão bibliográfica de 8 estudos coletados nas bases de dados, BVS e Cochrane. A otite média (OM) é uma das infecções mais comuns em crianças pequenas, e, embora a maioria dos casos seja tratada com sucesso, algumas complicações podem surgir, como a mastoidite. Este estudo tem por objetivo analisar a incidência de mastoidite em lactentes com otite média, identificar os fatores de risco para essa evolução e discutir as principais razões para o desenvolvimento dessa complicação grave, incluindo o uso inadequado de antibióticos.