bluuebunny/crossref_metadata_2025 huggingface dataset:
Menciptakan Peluang Usaha dengan Melihat Potensi Wilayah Melalui Program Kewirausahaan di Kelurahan Jati Luhur Kecamatan Jati Asih Kota Bekasi
Authors: Woelandari PG Dewi Sri , Setyawati Novita Wahyu
Published: March 2022

Kegiatan Pengabdian pada Masyarakat ini bertujuan untuk membangun kemandirian masyarakat agar dapat melihat potensi lingkungan sekitarnya terutama pada program kewirausahaan untuk mendapatkan manfaat secara langsung. Metode kegiatan pengabdian masyarakat ini menggunakan metode ceramah atau penyuluhan dan pembelajaran yang dipraktekkan oleh peserta dan nara sumber. Metode ceramah digunakan untuk menyampaikan pengetahuan secara umum tentang kewirausahaan, dan stop narkoba disertai dengan sesi tanya jawab. Metode pembelajaran digunakan untuk alih pengetahuan atau ketrampilan dan system nilai yang dimiliki oleh nara sumber kepada anggota masyarakat.Pelatihan ini melibatkan dosen Universitas Bhayangkara Jakarta Raya Bekasi yang bekerja sama dengan warga masyarakat Kel. Jati Luhur, Kec. Jati Asih Kota Bekasi sebagai subyek sasaran. Kegiatan pengabdian masyarakat dijadwalkan pada bulan Juli-Agustus 2019.
Penyuluhan Pendampingan Orang Tua Terhadap Putra/Putri Dalam Penggunaan Bijak Bersosmed
Authors: Setyawati Novita Wahyu , Supriyanto Supriyanto , Dharmanto Agus
Published: March 2022

Kegiatan Pengabdian pada Masyarakat ini bertujuan untuk memberikan edukasi, pengetahuan kepada orang tua terhadap putra/putrinya dalam penggunanaan media sosial yang bijak.. Metode yang digunakan dalam kegiatan pengabdian masyarakat ini menggunakan, pertama ceramah dengan menggunakan power point slide untuk menjelaskan kepada para peserta pengetahuan umum tentang bagaimana mendampingi dalam menggunakan media sosial, kedua berbagi pengalaman dengan melakukan diskusi dan sesi tanya jawab. Metode pembelajaran digunakan untuk berbagi pengetahuan dan sistem nilai yang dimiliki oleh nara sumber kepada anggota masyarakat. Pelatihan ini melibatkan dosen dan Mahasiswi Fakultas Ekonomi dan Bisnis, dengan masyarakat RT.005/ RW.001, Kel. Marga Mulya, Kec. Bekasi Utara Kota Bekasi.
Pencegahan Penyakit Pada Ternak Ayam Kampung Melalui Bimbingan Teknis Manajemen Sanitasi dan Biosecurity di Kecamatan Abeli
Authors: Libriani Restu , Nafiu La Ode , Saili Takdir , Abadi Musram , Sulfitrana Andini , Salido Wa Laili , Isnaeni Purnaning Dhian
Published: March 2022

Biosecurity is an integral concept that influences the success of livestock production systems, especially in reducing the risks and consequences of infectious and non-communicable diseases. The problem faced by breeders in Abeli District is the lack of knowledge of breeders about maintenance systems that meet the eligibility standards of commercial native chicken farms, handling biosecurity, sanitation and animal health against diseases that are not yet optimal, so that native village chickens are often exposed to various diseases. Through the Thematic KKN FPt UHO program, efforts should be made to improve the knowledge and skills of farmers about bisecurity management that meets the standards for commercial village chicken farms. This service is carried out for one month, starting from June 13, 2020 to July 13, 2020, Abeli District, Kendari City. This community service activity was carried out by involving Thematic KKN FPt UHO participants. The program is carried out in the form of Technical Guidance and Counseling, namely (1) Bimtek and virtual / online counseling; and (2) Bimtek and counseling using face-to-face methods both individually and more and still pay attention to the Covid-19 prevention protocol. The material for this service program is handling biosecurity, sanitation and animal health. The results that have been achieved in the Thematic KKN FPt UHO program are improving the knowledge and skills of breeders in biosecurity management procedures which include three main activities namely isolation, traffic control and sanitation as an effort to prevent transmission of disease in native chicken farms
Sinergitas Masyarakat pada Pembuatan Alat Pelindung Diri Tepat Guna Dalam Rangka Pencegahan dan Pengendalian Penyebaran Covid-19 di Wilayah Provinsi Sulawesi Tenggara
Authors: Faslih Arman , Jaya Siti Nawal , Al-Ikhsan Ainussalbi , Umar Muhammad Zakaria , Nangi Jumadil
Published: March 2022

Program Pendidikan Vokasi (PPV)-UHO sesuai dengan kapasitas aplikatifnya dan melalui Kuliah Kerja Nyata Tematik Penanggulangan Covid-19 Tahun 2020 turut berpartisipasi menghadapi wabah pandemi COVID-19 di Kota Kendari. Para mahasiswa dan warga binaannya mengumpulan donasi bahan-bahan bekas-pakai rumah tangga. Donasi tersebut dibuat oleh peserta KKN per individu berupa logistik APD secara tepat guna dan kebutuhan APD untuk masyarakat. APD ini akan disalurkan di daerah mahasiswa berada dan gugus tugas percepatan penanganan COVID-19 setempat. Pengabdian ini ditujukan untuk membuat logistik APD tepat guna dalam rangka pencegahan dan pengendalian COVID-19 di Sulawesi Tenggara. Metode pelaksanaan terdiri dari persiapan dan rekruitmen peserta, pembekalan mahasiswa, serta pelaksanaan. Program pengabdian kepada masyarakat terintegrasi KKN tematik 2020 disimpulkan sebagai berikut: pertama, para mahasiswa berkoordinasi dengan tokoh masyarakat setempat; kedua, para mahasiswa mengumpulkan bahan-bahan kerja pada masyarakat; ketiga para mahasiswa membuat APD bersama warga binaan; keempat, para mahasiswa menyalurkan produk APD ke masyarakat; kelima, produk APD yang dibuat oleh para mahasiswa dan warga binaan melebihi target yang direncanakan sebesar 68,2%. Kata Kunci: Alat Pelindung Diri, Covid-19
Penyiapan Desa Wansugi Kecamatan Kabangka Kabupaten Muna sebagai Desa Siaga Covid-19
Authors: Abapihi Bahriddin , Mukhsar Mukhsar , Baharuddin Baharuddin , Usman Ida , Sani Asrul
Published: March 2022

Corona Virus Disease atau COVID-19 telah mewabah ke seluruh dunia, termasuk Indonesia. Diperlukan pengetahuan, sikap, dan tindakan yang tepat seluruh lapisan masyarakat. Namun, tidak semua lapisan masyarakat paham dan sadar akan bahaya penyakit ini, khususnya masyarakat di perdesaan. Perlu menyiapkan masyarakat perdesaan dalam menghadapi wabah COVID-19, pemberian informasi yang cukup tentang untuk menghambat laju penyebarannya. Dalam program pengabdian ini dilakukan survey awal tentang pengetahuan, sikap, dan tindakan sebelum dilakukannya penyuluhan dan pemberian informasi kepada masyarakat. Proses pembelajaran kepada masyarakat dilakukan dengan memberikan informasi lengkap dengan bahasa yng mudah dipahami masyarakat setempat. Proses edukasi ini, kembali dilakukan survey untuk mengetahui pengetahuan, sikap, dan tindakan masyarakat setempat terhadap COVID-19. Hasil survey menunjukkan adanya peningkatan pengetahuan tentang COVID-19, serta peningkatan sikap dan tindakan positif pasca edukasi. Keywords: COVID-19, survey, desa siaga, penyuluhan, edukasi
Pelatihan Pembuatan Soal Evaluasi Pembelajaran Menggunakan Google Form Bagi Guru-Guru SMPN 8 Konawe Selatan
Authors: Wibawa Gusti Ngurah Adhi , Agusrawati Agusrawati , Makkulau Makkulau , Yahya Irma
Published: March 2022

Kebijakan belajar dari rumah akibat pandemi Covid-19 mengakibatkan proses pembelajaran dilakukan secara daring atau non tatap muka. Kondisi ini mengakibatkan perlu metode alternatif penggunaan metode pembelajaran dan evaluasinya. Pengabdian kepada masyarakat ini bertujuan untuk mengadakan pelatihan pembuatan soal evaluasi pembelajaran menggunakan Google Form bagi guru-guru SMPN 8 Konawe Selatan. Dari hasil pemantauan dan pendampingan tim pengabdian kepada peserta selama pelatihan, memperlihatkan bahwa ada perubahan kemampuan peserta dalam membuat soal. Jika sebelum pelatihan mereka membuat soal secara manual dan belum mengatahui bahwa soal dapat dibuat secara online melalui Google Form, maka setelah pelatihan mereka dapat membuat soal melalui Google Form yang nantinya akan dijawab secara online dan bagaimana cara melakukan rekapitulasi nilai yang sudah tersedia di Google Drive. Dari hasil evaluasi pelaksanaan pelatihan, dengan skor yang diberikan peserta sebesar 96,27% mengindikasikan bahwa peserta menunjukkan reaksi positif yang tinggi terhadap pelaksanaan pelatihan.
Pendampingan Teknis Seleksi Induk dan Penjantan Unggul pada Ayam Kampung Kelurahan Labibia Kecamatan Mandonga Kota Kendari
Authors: Nafiu La Ode , Saili Takdir , Arsad Sani La Ode , Abadi Musram , Sulfitrana Andini , Libriani Restu , Dewi S Rachmita , Fajar Ahmad
Published: March 2022

Kebutuhan masyarakat terhadap daging ayam lokal cenderung mengalami peningkatan yang diindikasikan dengan semakin berkembangnya industri kuliner dan bertambahnya restoran maupun rumah makan yang menyajikan daging ayam lokal khususnya di kota-kota besar. Kelurah Labibia merupakan salah satu wilayah di Kota Kendari yang masih memiliki peluang yang sangat besar untuk pengembangan ayam kampung. Peternak di Kelurahan Labibia Kecamatan Mandonga Kota Kendari memanfaatkan pekarangan rumah, untuk usaha budidaya ayam kampung. Kurangnya pengetahuan dan pemahaman peternak dalam pemilihan induk dan pejantan unggul menjadi masalah utama yang di hadapi oleh peternak di Kelurahan Labibia Kecamatan Mandonga Kota Kendari. Selain pakan dan manajemen pemelihraan, bibit juga merupakan faktor penentu dalam pengembangan budidaya ayam kampung. Peningkatan pengetahuan dan keterampilan peternak perlu dilakukan, penyuluhan tentang tata cara atau metode seleksi indukan dan pejantan unggul melalui Program Bina Desa Fakultas Peternakan Universitas Halu Oleo. Pelaksanaan Program Bina Desa Fakultas Peternakan Universitas Halu Oleo dilaksanakan di Kelurahan Labibia Kecamatan Mandonga Kota Kendari. Pelaksanaan program dilakukan dalam bentuk penyuluhan baik secara massal, kelompok dan individu dengan tetap memperhatikan protokol pencegahan Covid-19. Materi penyululuhan meliputi penguatan kelembagaan dan metode seleksi indukan dan pejantan unggul. Berdasarkan kegiatan yang telah dilaksanakan, hasil yang telah dicapai pada Program Bina Desa di Kelurahan Labibia Kecamatan Mandonga Kota Kendari  melalui penyuluhan dan bimbingan teknis manajemen seleksi ayam kampung meningkatkan pengetahuan dan pemahaman kelompok ternak sehingga peternak dapat meningkatkan produktifitas ayam kampung
Pengembangan Infrastruktur Sipil Berbasis Lingkungan Pasca Pandemi Covid-19 di Kelurahan Anduonohu Kota Kendari
Authors: Welendo La , Ahmad Siti Nurjanah , Mursidi Baso , Nasrul Nasrul , Nuhun Ridwan Syah , Putra Adris A.
Published: March 2022

The realization of an increase in the community's economy with access to roads, especially on roads in theAnduonohu Urban Village, Kendari City, which are still limited in the formation of roads, as a form of highereducation tridarma and shared responsibility for the benefit of the community, the purpose of carrying out thisservice is to provide understanding and application of infrastructure development civilian construction ofenvironmental roads during the post-Covid-19 pandemic to facilitate access to community mobility in thatenvironment and so that people can understand the importance of road infrastructure for the sustainability ofthe economy of the surrounding community.The process of implementing this service activity is the application of a technique for making newenvironmental roads and repairing existing footpaths according to the available resources of the communityservice implementing TEAM, namely socialization to the community by paying attention to work patternsduring the Covid-19 pandemic according to standard health protocols set. The road construction implementationtechnique conveyed in this service activity is in the form of increasing public knowledge, especially in theprocedural implementation of civil infrastructure, as well as maintaining the cleanliness and health of theenvironment around their residence.The process of implementing this service activity is the application of atechnique for making new environmental roads and repairing existing footpaths according to the availableresources of the community service implementing TEAM, namely socialization to the community by payingattention to work patterns during the Covid-19 pandemic according to standard health protocols set. The roadconstruction implementation technique conveyed in this service activity is in the form of increasing publicknowledge, especially in the procedural implementation of civil infrastructure, as well as maintaining thecleanliness and health of the environment around their residence. The method used in this activity is sampleprovide, direct and face-to-face discussions with the community, as well as field applications in the developmentof civil infrastructure in Anduonohu Village, Kendari City. The results obtained by this community serviceprogram are that the community can understand the importance of science and the manufacture ofenvironmental road physical infrastructure, the community has a fairly good response and high enthusiasm forthe implementation of this program of activities, it can benefit the community by forming environmental roadswith more pavement. both for the movement / mobility of the community in that location and the locationaround it. Keywords: Civil Infrastructure, Environmental Road, Community, Covid-19 Pandemic
Diversifikasi Pengolahan Kulit Sapi pada Kelompok Usaha Suka Mandiri di Kelurahan Rahandouna Kecamatan Poasia Kota Kendar
Authors: Fitrianingsih Fitrianingsih , Abadi Musram , Sulfitrana Andini , Kimestri Asma Bio
Published: March 2022

Kegiatan Program Kemitraan Masyarakat Internal (PKM-I) UHO 2021 ditujukan pada Kelompok Usaha Suka Mandiri yang dilaksanakan di Kecamatan Poasia, Kota Kendari, Provinsi Sulawesi Tenggara. Kelompok Suka Mandiri merupakan merupakan salah satu kelompok usaha yang bergerak dalam bidang produksi kerupuk kulit yang ada di Kecamatan Poasia, yang berdiri sejak tahun 2012. Berdasarkan investigasi di lapangan proses produksi kerupuk kulit di kelompok Suka Mandiri masih dilakukan secara sederhana, dengan mengesampingkan cara produksi pangan yang baik, seperti fasilitas dan peralatan produksi yang seadanya dan tidak terpelihara dengan baik, serta tidak menerapkan higieni karyawan, dikuatirkan keadaan ini akan menurunkan kualitas produk. Melalui kegiatan Program Kemitraan Masyarakat (PKM) ini, tim pelaksana kegiatan berperan serta aktif dalam membantu meningkatkan pemahaman dan keterampilan masyarakat mengenai pengolahan kuliat yang aman dan sehat serta penganekaragangan produk kulit dengan berbagai rasa. Program PKM-I ini dibagi dalam 2 kegiatan, yaitu: (1) Kegiatan non fisik meliputi: penyuluhan, pelatihan dan bimbingan teknis (pendampingan) sebagai media transfer informasi pengetahuan dan teknologi; (2) Kegiatan fisik melalui: pengadaan alat/bahan, demontrasi pengolahan kulit sapi yang baik dan benar. Pelaksanaan program dilakukan dalam bentuk penyuluhan dan bimbingan teknis dengan metode tatap muka baik secara individu, kelompok, ataupun massal dengan tetap memperhatikan protokol pencegahan Covid-19. Berdasarkan hasil pengabdian masyarakat melalui program PKM-I UHO 2021 yang telah dilakukan terhadap industri lokal yaitu pada kelompok Usaha Suka Mandiri dapat disimpulkan bahwa (1) kelompok mitra telah memahami pengolahan kulit sapi dengan menerapkan sistem pangan yang aman dan sehat, (2) kelompok mitra mampu melakukan diversifikasi atau penganekaragaman produk olahan kulit sapi, (3) kulit yang diolah telah menghasilakn aneka macam produk, seperti kerupuk kulit aneka rasa seperti pedas, manis, balado, jagung bakar, keju, dan rasa barbeque.
Pendampingan Pembuatan Bahan Ajar Digital Interaktif Menggunakan Aplikasi Sigil Untuk Meningkatkan Kompetensi Profesional Guru Sma/Smk Di Kota Kendari
Authors: Arman Arman , Jufra Jufra , Ransi Natalis , Cahyono Edi , Eso Rosliana
Published: March 2022

Dalam kondisi pandemi COVID-19 ini, untuk meningkatkan kualitas pembelajaran diperlukan media pembelajaran yang merupakan suatu alat dan bahan yang dapat dipakai untuk mencapai tujuan pendidikan. Media pembelajaran di sekolah saat ini bergeser menjadi pembelajaran yang memanfaatkan teknologi informasi dan komunikasi (TIK), Karena itu guru –guru SMA/SMKdirasa perlu untuk melakukan inovasi pembelajaran. Kenyataan saat ini guru-guru SMA/SMKmasih jarang melakukan inovasi pembelajaran yang memanfaatkan TIK dengan menggunakan aplikasi software dalam kegiatan pembelajaran. Kegiatan pengabdian kepada masyarakat ini ditujukan untuk guru-guru SMA/SMK di Kota Kendari sebagai upaya untuk meningkatkan kompetensi dan profesionalitasnya dalam pembelajaran yang berbasis TIK. Kegiatan pengabdian ini bertujuan untuk meningkatkan keterampilan IT bagi guru-guru SMA/SMK di Kota Kendari melalui pembuatan bahan ajar digital interaktif menggunakan aplikasi SIGIL. Aplikasi SIGIL adalah aplikasi untuk manajemen dan pembuatan digital book dengan format epub, dimana kita dapat membuat digital book sesuai dengan yang kita ingikan. Sigil mendukung format text, html dan format epub Bahan ajar digital interaktif yang dihasilkan melalui SIGIL merupakan modul pembelajaran yang sangat berperan dalam meningkatkan kualitas pembelajaran di SMA/SMK. Mitra dari kegiatan pengabdian ini secara umum adalah guru-guru SMA/SMK di Kota Kendari. Tujuan umum kegiatan pengabdian ini adalah untuk meningkatkan peran dan fungsi guru-guru SMA/SMK di Kota Kendari dalam meningkatkan kompetensi, kinerja dan profesionalitas guru dalam pembelajaran. Untuk mencapai tujuan tersebut maka perlu dilakukan pendampingan dan pembimbingan pembuatan bahan ajar digital interaktif melalui aplikasi SIGIL. Metode yang digunakan dalam kegiatan pengabdian ini adalah: pembimbingan dan pendampingan serta memberikan materi dan dialog interaktif melalui demonstrasi / simulasi / praktik. Adapun langkah-laangkah yang dilakukan adalah: (1) Pembimbingan penggunaan aplikasi SIGIL krpada guru-guru SMA/SMK lingkup Kota Kendari. (2) Pembuatan bahan ajar berupa modul pembelajaran SMA/SMK dengan target peningkatan pemahaman kepada guru-guru dalam membuat bahan ajar online, (3) Pembuatan bahan ajar digital interaktif menggunakan aplikasi SIGIL dengan target peningkatan pemahaman guru dalam membuat bahan ajar digital interaktif. Kegiatan pengabdian ini menekankan pada penerapan IPTEK guna meningkatkan kompetensi guru-guru SMA/SMK di Kota Kendari melalui pembimbingan pembuatan bahan ajar digital interaktif dalam pembelajaran. Sedangkan target luaran kegiatan ini berupa modul dan e-modul pembelajaran di SMA/SMK. Hasil dari kegiatan pengabdian ini akan diterbitkan pada salah satau jurnal nasional dan juga media online.
ANALISIS EFEKTIVITAS PENGAWASAN INSPEKTORAT DAERAH KABUPATEN BOMBANA
Authors: Masnia Masnia , Basri Muhammad , Rahman Rahman
Published: March 2022

ABSTRAK Penelitian ini bertujuan untuk menganalisis dan mendefiniskan efektivitas pengawasan Inspektorat Daerah Kabupaten Bombana. Penelitian ini menggunakan pendekatan deskriptif kualitatif. Metode pengumpulan data terdiri dari wawancara, observasi dan studi dokumentasi. Informan dalam penelitian ini terdiri dari: inspektorat (sekretaris, Irban I, Irban II, Irban III, dan Irban IV, para Kasubag, Auditor dan Staf), serta bagian pengadaan barang jasa sekretariat daerah Kabupaten Bombana (keuangan, pembangunan, pemerintahan dan aparatur). Teknik pengumpulan data yang digunakan adalah observasi, wawancara, dan studi pustaka. Teknik analisis data yang digunakan adalah deskriptif kualitatif model interaktif yang mencakup reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa: sistem kerja pengawasan yang dilakukan oleh Inspektorat Daerah Kabupaten Bombana dengan efektif jika dianalsis berdasarkan indikator: a) unsur keakuratan, b) tepat waktu, c) terpusat, d) realistis secara ekonomis, d) realistis secara organisasional. Sehingga dapat diakatan bahwa Inspektorat daerah Kabupaten Bombana yang berfungsi sebagai lembaga pengawasan melalui tim auditor telah menjalankan amanah organisasi dengan efektiv dalam melaksanakan tugas dan fungsinya.Kata kunci : Efektivitas, Faktor Berpengaruh, dan Pengawasan Inspektorat Daerah
MODEL KOMUNIKASI PENANGANAN KEKERASAN PEREMPUAN STUDI DINAS PEMBERDAYAAN PEREMPUAN DAN PERLINDUNGAN ANAK
Authors: Indra Pratama Kiky Reskianti , Tarifu La , Abdullah Muhammad Zein
Published: March 2022

Penelitian ini bertujuan untuk mengetahui dan menganalisis model komunikasi penanganan kekerasan terhadap perempuan pada Dinas Pemberdayaan Perempuan Dan Perlindungan Anak Kota Kendari. Penelitian ini menggunakan pendekatan kualitatif dengan informan sebanyak 5 orang yang ditentukan menggunakan teknik purposive sampling. Adapun metode pengumpulan data dalam penelitian ini diperoleh melalui wawancara, studi dokumen, dan observasi. Data yang telah dikumpulkan dianalisis secara kualitatif melalui model analisis data interaktif yang terdiri atas reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa model komunikasi penanganan kekerasan terhadap perempuan pada Dinas Pemberdayaan Perempuan dan Perlindungan Anak Kota Kendari menggunakan model komunikasi interaksional yang menggambarkan komunikasi sirkuler yang berlangsung secara berputar dan interaktif antara para pelaku komunikasi yaitu perempuan korban kekerasan dengan petugas penanganan kekerasan terhadap perempuan dengan melibatkan mitra kerja yang menyediakan layanan rujukan yang tidak tersedia di Dinas Pemberdayaan Perempuan dan Perlindungan Anak Kota Kendari.Kata Kunci: Model Komunikasi, Kekerasan, Perempuan.
Delivering Singlet Oxygen in Dark Condition With an Anthracene-Functionalized Semiconducting Compound for Enhanced Phototheranostics
Authors: Shen Jian , Pan Liuhong , Zhang Xujing , Zou Zhenyuan , Wei Bo , Chen Yongchang , Tang Xiaoyan , Zou Dengfeng
Published: March 2022

Photodynamic therapy (PDT) utilizes the photogeneration of reactive oxygen species (ROS) with high cytotoxicity to kill cancer cells, holding great promise for cancer treatment. Fractionated delivery of singlet oxygen (1O2) is a wise approach to relieving hypoxia, thus enhancing the therapeutic efficacy. In this article, an anthracene-functionalized semiconducting compound (DPPA) has been designed and synthesized. With irradiation, the compound is able to undergo efficient intersystem crossing (ISC) and non-radioactive decay for photodynamic/photothermal synergistic therapy. In addition, the anthracene module is able to capture and release 1O2 reversibly with or without irradiation. DPPA nanoparticles (NPs) obtained by nanoprecipitation with DSPE-PEG exhibit considerable high phototoxicity on human kidney cancer cells (A498), and the half maximum inhibitory concentration (IC50) is 15.8 μg/ml. Furthermore, an in vivo study demonstrates that complete tumor suppression was observed when the mice were administered DPPA NPs with the help of laser, compared with the control and dark groups. The H&E analysis of the normal tissues (the heart, liver, spleen, lungs, and kidney) indicates that such NPs cause no side effects, indicating the biosafety of DPPA NPs. The results provide a strategy to design a heavy-atom–free photosensitizer for photothermal and fractionated PDT against kidney tumors.
Fermented Jussara: Evaluation of Nanostructure Formation, Bioaccessibility, and Antioxidant Activity
Authors: Giaconia Michele Amendoeira , Ramos Sergiana dos Passos , Fratelli Camilly , Assis Marcelo , Mazzo Tatiana Martelli , Longo Elson , de Rosso Veridiana Vera , Braga Anna Rafaela Cavalcante
Published: March 2022

Among the species of plants present in the Atlantic Forest, the jussara (Euterpe edulisMart.) stands out for the contents of bioactive compounds present in its composition. Fermentation processes can be essential in converting bioproducts and bioactive compounds, improving their biological properties. In addition, the improvement of procedures for the maintenance of the features of bioactive compounds has been a research focus in recent years, and the nanotechnology features that can potentially solve this issue have been highlighted among the most reviewed paths. The present work focused on tailoring nanostructures applying polyethylene oxide, assembling fermented jussara pulp nanofibers, and assessing their characteristics. The results revealed the formation of fermented jussara nanofibers with a diameter of 101.2 ± 26.2 nm. Also, the obtained results allow us to state that it is possible to maintain or even increase the antioxidant activity of anthocyanins and their metabolites after fermentation processes.
Mechanical Stimulation Protects Against Chondrocyte Pyroptosis Through Irisin-Induced Suppression of PI3K/Akt/NF-κB Signal Pathway in Osteoarthritis
Authors: Jia Shuangshuo , Yang Yue , Bai Yishu , Wei Yingliang , Zhang He , Tian Yicheng , Liu Jiabao , Bai Lunhao
Published: March 2022

Irisin, a myokine secreted by muscle during physical exercise, is known to have biological activities in different cell types. Chondrocyte inflammation and pyroptosis have been shown to play important roles in osteoarthritis (OA). In this study, we investigated the effects of exercise-induced irisin during different intensities of treadmill exercise in a rat OA model and the anti-inflammatory and antipyroptosis mechanism of irisin in OA chondrocytes. Forty-eight SD rats (n = 8) were randomly assigned to control (CG), OA (OAG), OA groups under different intensities of treadmill exercise (OAL, OAM, and OAH), OAM + irisin neutralizing antibodies group (OAM + irisin (NA)). The levels of irisin and the severity of OA between groups were detected using ELISA, histology, immunohistochemistry, X-ray and computed tomography and magnetic resonance imaging. The anti-inflammatory and antipyroptosis mechanisms of irisin were investigated in vitro in OA chondrocytes preincubated with recombinant irisin (0, 5, or 10 ng/ml) for 1 h before treatment with interleukin-1β (IL-1β) for 24 h mRNA and protein expression levels were determined using quantitative reverse transcription polymerase chain reaction, and western blot analyses. Morphological changes and cell death associated with pyroptosis were examined using transmission electron microscopy, flow cytometry and immunofluorescence. Moderate-intensity treadmill exercise increased the levels of irisin, exhibiting the best therapeutic effects on OA which could be suppressed by irisin neutralizing antibodies. Irisin not only recovered the expression of collagen II and attenuated that of MMP-13 and ADAMTS-5 in IL-1β-induced OA chondrocytes by inhibiting the PI3K/Akt/NF-κB signaling pathway, but also inhibited the activity of nod-like receptor protein-3 (NLRP3)/caspase-1, thus ameliorating pyroptosis in chondrocytes. In conclusion, moderate mechanical stimulation protects against chondrocyte pyroptosis through irisin-induced suppression of PI3K/Akt/NF-κB signal pathway in osteoarthritis.
Construction and Validation of Two Hepatocellular Carcinoma-Progression Prognostic Scores Based on Gene Set Variation Analysis
Authors: He Qifan , Fan Baorui , Du Peng , Jin Yonghai
Published: March 2022

Background: Liver hepatocellular carcinoma (LIHC) remains a global health challenge with a low early diagnosis rate and high mortality. Therefore, finding new biomarkers for diagnosis and prognosis is still one of the current research priorities.

Methods: Based on the variation of gene expression patterns in different stages, the LIHC-development genes (LDGs) were identified by differential expression analysis. Then, prognosis-related LDGs were screened out to construct the LIHC-unfavorable gene set (LUGs) and LIHC-favorable gene set (LFGs). Gene set variation analysis (GSVA) was conducted to build prognostic scoring models based on the LUGs and LFGs. ROC curve analysis and univariate and multivariate Cox regression analysis were carried out to verify the diagnostic and prognostic utility of the two GSVA scores in two independent datasets. Additionally, the key LCGs were identified by the intersection analysis of the PPI network and univariate Cox regression and further evaluated their performance in expression level and prognosis prediction. Single-sample GSEA (ssGSEA) was performed to understand the correlation between the two GSVA enrichment scores and immune activity.

Result: With the development of LIHC, 83 LDGs were gradually upregulated and 247 LDGs were gradually downregulated. Combining with LIHC survival analysis, 31 LUGs and 32 LFGs were identified and used to establish the LIHC-unfavorable GSVA score (LUG score) and LIHC-favorable GSVA score (LFG score). ROC curve analysis and univariate/multivariate Cox regression analysis suggested the LUG score and LFG score could be great indicators for the early diagnosis and prognosis prediction. Four genes (ESR1, EHHADH, CYP3A4, and ACADL) were considered as the key LCGs and closely related to good prognosis. The frequency of TP53 mutation and copy number variation (CNV) were high in some LCGs. Low-LFG score patients have active metabolic activity and a more robust immune response. The high-LFG score patients characterized immune activation with the higher infiltration abundance of type I T helper cells, DC, eosinophils, and neutrophils, while the high-LUG score patients characterized immunosuppression with the higher infiltration abundance of type II T helper cells, TRegs, and iDC. The high- and low-LFG score groups differed significantly in immunotherapy response scores, immune checkpoints expression, and IC50 values of common drugs.

Conclusion: Overall, the LIHC-progression characteristic genes can be great diagnostic and prognostic signatures and the two GSVA score systems may become promising indices for guiding the tumor treatment of LIHC patients.
Insights Into Mechanisms of Oriented Division From Studies in 3D Cellular Models
Authors: Donà Federico , Eli Susanna , Mapelli Marina
Published: March 2022

In multicellular organisms, epithelial cells are key elements of tissue organization. In developing tissues, cellular proliferation and differentiation are under the tight regulation of morphogenetic programs, that ensure the correct organ formation and functioning. In these processes, mitotic rates and division orientation are crucial in regulating the velocity and the timing of the forming tissue. Division orientation, specified by mitotic spindle placement with respect to epithelial apico-basal polarity, controls not only the partitioning of cellular components but also the positioning of the daughter cells within the tissue, and hence the contacts that daughter cells retain with the surrounding microenvironment. Daughter cells positioning is important to determine signal sensing and fate, and therefore the final function of the developing organ. In this review, we will discuss recent discoveries regarding the mechanistics of planar divisions in mammalian epithelial cells, summarizing technologies and model systems used to study oriented cell divisions in vitro such as three-dimensional cysts of immortalized cells and intestinal organoids. We also highlight how misorientation is corrected in vivo and in vitro, and how it might contribute to the onset of pathological conditions.
Mitochondrial Fission and Fusion in Tumor Progression to Metastasis
Authors: Boulton Dillon P. , Caino M. Cecilia
Published: March 2022

Mitochondria are highly dynamic organelles which can change their shape, via processes termed fission and fusion, in order to adapt to different environmental and developmental contexts. Due to the importance of these processes in maintaining a physiologically healthy pool of mitochondria, aberrant cycles of fission/fusion are often seen in pathological contexts. In this review we will discuss how dysregulated fission and fusion promote tumor progression. We focus on the molecular mechanisms involved in fission and fusion, discussing how altered mitochondrial fission and fusion change tumor cell growth, metabolism, motility, and invasion and, finally how changes to these tumor-cell intrinsic phenotypes directly and indirectly impact tumor progression to metastasis. Although this is an emerging field of investigation, the current consensus is that mitochondrial fission positively influences metastatic potential in a broad variety of tumor types. As mitochondria are now being investigated as vulnerable targets in a variety of cancer types, we underscore the importance of their dynamic nature in potentiating tumor progression.
Targeting Integrins for Cancer Therapy - Disappointments and Opportunities
Authors: Bergonzini Cecilia , Kroese Kim , Zweemer Annelien J. M. , Danen Erik H. J.
Published: March 2022

Integrins mediate adhesive interactions between cells and their environment, including neighboring cells and extracellular matrix (ECM). These heterodimeric transmembrane receptors bind extracellular ligands with their globular head domains and connect to the cytoskeleton through multi-protein interactions at their cytoplasmic tails. Integrin containing cell–matrix adhesions are dynamic force-responsive protein complexes that allow bidirectional mechanical coupling of cells with their environment. This allows cells to sense and modulate tissue mechanics and regulates intracellular signaling impacting on cell faith, survival, proliferation, and differentiation programs. Dysregulation of these functions has been extensively reported in cancer and associated with tumor growth, invasion, angiogenesis, metastasis, and therapy resistance. This central role in multiple hallmarks of cancer and their localization on the cell surface makes integrins attractive targets for cancer therapy. However, despite a wealth of highly encouraging preclinical data, targeting integrin adhesion complexes in clinical trials has thus far failed to meet expectations. Contributing factors to therapeutic failure are 1) variable integrin expression, 2) redundancy in integrin function, 3) distinct roles of integrins at various disease stages, and 4) sequestering of therapeutics by integrin-containing tumor-derived extracellular vesicles. Despite disappointing clinical results, new promising approaches are being investigated that highlight the potential of integrins as targets or prognostic biomarkers. Improvement of therapeutic delivery at the tumor site via integrin binding ligands is emerging as another successful approach that may enhance both efficacy and safety of conventional therapeutics. In this review we provide an overview of recent encouraging preclinical findings, we discuss the apparent disagreement between preclinical and clinical results, and we consider new opportunities to exploit the potential of integrin adhesion complexes as targets for cancer therapy.
Left Atrial Function Post Radiofrequency and Cryoballoon Ablation Assessed by Volume-Pressure Loops
Authors: Karanasos Antonios , Tyrovolas Konstantinos , Tsiachris Dimitrios , Efremidis Michalis , Kordalis Athanasios , Karmpalioti Maria , Prappa Efstathia , Karagiannis Stefanos , Aggeli Constantina , Gatzoulis Konstantinos , Tousoulis Dimitrios , Tsioufis Costas , Toutouzas Konstantinos P.
Published: March 2022

Background

Left atrial (LA) function is linked to atrial fibrillation (AF) pathogenesis. AF catheter ablation decreases disease burden with potentially favorable effects on cardiac function. Atrial volume-pressure loops can optimally assess the LA function.

Objective

To investigate changes in LA function by volume-pressure loops after paroxysmal AF ablation and explored potential differences between the radiofrequency and cryoballoon ablation.

Methods

We analyzed 44 patients undergoing paroxysmal AF ablation from 2 centers, 22 treated with radiofrequency and 22 with cryoablation. Pre- and post-procedure, all patients underwent a real-time three-dimensional transthoracic ECG to evaluate LA volume, while simultaneously recording LA pressure following transseptal puncture. Volume-pressure loops pre- and post-procedure were created by paired data. Areas of A-loop (LA booster pump function) and V-loop (LA reservoir function), and the stiffness constant determining the slope of the exponential curve during LA filling were calculated.

Results

Average LA pressure, A-wave amplitude, and V-wave amplitude were increased post-procedurally (p < 0.001). Overall, A-loop area decreased (p = 0.001) and V-loop area tended to increase (p = 0.07). The change in both A-loop and V-loop areas was similar between radiofrequency- and cryoballoon-treated patients (p = 0.18 and p = 0.52, respectively). However, compared with cryoballoon-treated patients, radiofrequency-treated patients had higher increase in the stiffness constant (b = 0.059; 95% CI: 0.022–0.096; p = 0.006).

Conclusion

AF catheter ablation by the radiofrequency or cryoballoon is associated with the decrease of the booster pump function and increase of the reservoir function. Moreover, there is a post-procedural increase of LA pressure which is associated with an acute increase in LA stiffness in radiofrequency ablation, but not in cryoablation.
Long-Lasting Myocardial and Skeletal Muscle Damage Evidenced by Serial CMR During the First Year in COVID-19 Patients From the First Wave
Authors: Filippetti Laura , Pace Nathalie , Louis Jean-Sebastien , Mandry Damien , Goehringer François , Rocher Maria-Soledad , Jay Nicolas , Selton-Suty Christine , Hossu Gabriela , Huttin Olivier , Marie Pierre-Yves
Published: March 2022

Introduction

This observational CMR study aims to characterize left-ventricular (LV) damage, which may be specifically attributed to COVID-19 and is distant in time from the acute phase, through serial CMR performed during the first year in patients with no prior cardiac disease.

Methods

This study included consecutive patients without any prior history of cardiac disease but with a peak troponin-Ic > 50 ng/ml at the time of the first COVID-wave. All had a CMR in the first months after the acute phase, and some had an additional CMR at the end of the first year to monitor LV function, remodeling, and abnormalities evocative of myositis and myocarditis - i.e., increased T1/T2 relaxation times, increased extracellular volume (ECV), and delayed contrast enhancement.

Results

Nineteen consecutively admitted COVID-19 patients (17 men, median age 66 [57–71] years) were included. Eight (42%) had hypertension, six (32%) were obese, and 16 (84%) had suffered an acute respiratory distress syndrome. The 1st CMR, recorded at a median 3.2 [interquartile range: 2.6–3.9] months from the troponin peak, showed (1) LV concentric remodeling in 12 patients (63%), (2) myocardial tissue abnormalities in 11 (58%), including 9 increased myocardial ECVs, and (3) 14 (74%) increased ECVs from shoulder skeletal muscles. The 2nd CMR, obtained at 11.1 [11.0–11.7] months from the troponin peak in 13 patients, showed unchanged LV function and remodeling but a return to normal or below the normal range for all ECVs of the myocardium and skeletal muscles.

Conclusion

Many patients with no history of cardiac disease but for whom an increase in blood troponin-Ic ascertained COVID-19 induced myocardial damage exhibited signs of persistent extracellular edema at a median 3-months from the troponin peak, affecting the myocardium and skeletal muscles, which resolved within a one-year time frame. Associations with long-COVID symptoms need to be investigated on a larger scale now.

Clinical Trial Registration

NCT04753762 on the ClinicalTrials.gov site.
Phosphorus Cycle and Primary Productivity Changes in the Tethys Ocean During the Permian-Triassic Transition: Starving Marine Ecosystems
Authors: Müller Johann , Sun Yadong , Yang Fen , Fantasia Alicia , Joachimski Michael
Published: March 2022

The ultimate cause(s) of the end-Permian mass extinction (∼252 Ma ago) has been disputed. A complex interplay of various effects, rather than a single, universal killing mechanism, were most likely involved. Climate warming as consequence of greenhouse gas emissions by contemporaneous Siberian Traps volcanism is widely accepted as an initial trigger. Synergetic effects of global warming include increasing stratification of the oceans, inefficient water column mixing, and eventually low marine primary productivity culminating in a series of consequences for higher trophic levels. To explore this scenario in the context of the end-Permian mass extinction, we investigated sedimentary total organic carbon, phosphorus speciation as well as nickel concentrations in two low-latitude Tethyan carbonate sections spanning the Permian-Triassic transition. Total organic carbon, reactive phosphorus and nickel concentrations all decrease in the latest Permian and are low during the Early Triassic, pointing to a decline in primary productivity within the Tethyan realm. We suggest that the productivity collapse started in the upper C. yini conodont Zone, approximately 30 ka prior to the main marine extinction interval. Reduced primary productivity would have resulted in food shortage and thus may serve as explanation for pre-mass extinction perturbations among marine heterotrophic organisms.
Depositional Record and Geochemistry Constraints on the Late Miocene–Quaternary Evolution of the Taiyuan Basin in Shanxi Rift System, China
Authors: Zhuang Qitian , Wei Rongzhu , He Honglin
Published: March 2022

The formation and evolution of the Shanxi Rift System are topics of interest because of its unique geometry and critical position in the North China Plate. However, it is not easy to delineate the late Cenozoic tectonic processes. This is partly because it is difficult to preserve the normal faults’ movement evidence and related deposits for dating in the outcrop. Besides, the current studies are heavily weighted in the low-temperature thermochronology, whereas it is insensitive to the exhumation after the late Miocene. To decipher the late Cenozoic evolution, we offer a case of detailed depositional recording from the boreholes of the Taiyuan Basin in the Shanxi Rift System. Fluvial, deltaic, and lacustrine environments were identified by the systematic stratigraphic analysis of boreholes. Two mega transgressions generated basin-wide lakes at ca. 5.8–4.4 and ca. 2.2–1.6 Ma. We attribute the lake expansion events to tectonism because climatic variations from reconstruction of the paleoclimate with geochemistry index and previous work exhibited asynchronous processes with the transgression. Thus, the basin experienced two stages of extensive subsidence after the rift occurred. This result is conducive to obtaining a more complete late Cenozoic tectonic evolution of the Shanxi Rift System.
Gamifying Virtual Exploration of the Past 350 Million Years of Vertebrate Evolution
Authors: Mead Chris , Bruce Geoffrey , Taylor Wendy , Buxner Sanlyn , Anbar Ariel D.
Published: March 2022

Surviving Extinction is an interactive, adaptive, digital learning experience through which students learn about the history of vertebrate evolution over the last 350 million years. This experience is self-contained, providing students with immediate feedback. It is designed to be used in a wide range of educational settings from junior high school (∼12 years old) to university level. Surviving Extinction’s design draws on effective aspects of existing virtual field trip-based learning experiences. Most important among these is the capacity for students to learn through self-directed virtual explorations of simulated historical ecosystems and significant modern-day geologic field sites. Surviving Extinction also makes significant innovations beyond what has previously been done in this area, including extensive use of gamified elements such as collectibles and hidden locations. Additionally, it blends scientifically accurate animations with captured media via a user interface that presents an attractive, engaging, and immersive experience. Surviving Extinction has been field-tested with students at the undergraduate, high school, and pre-high school levels to assess how well it achieves the intended learning outcomes. In all settings we found significant gains pre- to post-activity on a knowledge survey with medium to large effect sizes. This evidence of learning is further supported with data from the gamified elements such as the number of locations discovered and total points earned. Surviving Extinction is freely available for use and detailed resources for educators are provided. It is appropriate for a range of undergraduate courses that cover the history of life on Earth, including ones from a biology, ecology, or geology perspective and courses for either majors or non-majors. Additionally, at the high school level, Surviving Extinction is directly appropriate to teaching adaptation, one of the disciplinary core ideas in the Next Generation Science Standards. Beyond providing this resource to the educational community, we hope that the design ideas demonstrated in Surviving Extinction will influence future development of interactive digital learning experiences.
Paper and Salt: Biodegradable NaCl-Based Humidity Sensors for Sustainable Electronics
Authors: Falco Aniello , Marín-Sánchez Antonio , Loghin Florin C. , Castillo Encarnación , Salinas-Castillo Alfonso , Salmerón José F. , Rivadeneyra Almudena
Published: March 2022

Flexible and thin-film humidity sensors are currently attracting the attention of the scientific community due to their portability and reduced size, which are highly useful traits for use in the Internet o Things (IoT) industry. Furthermore, in order to perform efficient and profitable mass production, it is necessary to develop a cost-effective and reproducible fabrication process and materials. Green fabrication methods and biodegradable materials would also minimize the environmental impact and create a sustainable IoT development. In this paper, flexible humidity sensors based on a common salt (NaCl) sensing layer are reported. Our sensors and the fabrication techniques employed, such as dip and spray coating, provide a biodegradable, low cost, and highly reproducible device. One of the sensors reported presents a typical resistive behaviour from 40% RH up to 85% RH with a sensitivity of −0.21 (Z/%RH). The performance of the sensors obtained with several fabrication techniques is studied and reported at multiple frequencies from 100 Hz to 10 MHz, showcasing its versatility and robustness.
The Role of Apelin–APJ System in Diabetes and Obesity
Authors: Li Cheng , Cheng Hongna , Adhikari Binay Kumar , Wang Shudong , Yang Na , Liu Wenyun , Sun Jian , Wang Yonggang
Published: March 2022

Nowadays, diabetes and obesity are two main health-threatening metabolic disorders in the world, which increase the risk for many chronic diseases. Apelin, a peptide hormone, exerts its effect by binding with angiotensin II protein J receptor (APJ) and is considered to be linked with diabetes and obesity. Apelin and its receptor are widely present in the body and are involved in many physiological processes, such as glucose and lipid metabolism, homeostasis, endocrine response to stress, and angiogenesis. In this review, we summarize the literatures on the role of the Apelin–APJ system in diabetes and obesity for a better understanding of the mechanism and function of apelin and its receptor in the pathophysiology of diseases that may contribute to the development of new therapies.