bluuebunny/crossref_metadata_2025 huggingface dataset:
Dose-Dependent Effects of Zoledronic Acid on Human Periodontal Ligament Stem Cells: An In Vitro Pilot Study
Authors: Di Vito Anna , Chiarella E. , Baudi F. , Scardamaglia P. , Antonelli A. , Giudice D. , Barni T. , Fortunato L. , Giudice A.
Published: October 2020

Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs’ well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and osteocyte behavior. The aim of this study is to evaluate the biological effects of zoledronic acid (ZOL) in an in vitro model of periodontal ligament stem cells (PDLSCs) by using an experimental setting that resembles the in vivo conditions. PDLSCs were treated with different concentrations of ZOL ranging from 0.1 to 5 μM. The effects of ZOL exposure were evaluated on cell viability via 3-[4,5-Dimethylthiaoly]-2,5-diphenyltetrazolium bromide (MTT), cell cycle analysis, apoptosis detection, and immunofluorescence. Quantitative real-time polymerase chain reaction (PCR), colorimetric detection of alkaline phosphatase activity, and Alizarin Red S staining were performed to investigate the osteogenic potential of PDLSCs exposed to ZOL. MTT analysis showed that the viability of PDLSCs exposed to ZOL concentration ≥1.5 μM for 3 and 6 days was significantly lower ( P < 0.001) than that of untreated cells. The percentage of apoptotic cells was significantly higher in PDLSCs exposed for 4 days to ZOL at 2 μM ( P < 0.01) and 5 μM ( P < 0.001) when compared to the control. Moreover, ZOL treatment (3 days) accounted for alterations in cell cycle distribution, with an increase in the proportion of cells in G0/G1 phase and a reduction in the proportion of cells in S phase. Chronic exposure (longer than 7 days) of PDLSCs to ZOL accounted for the downregulation of ALP, RUNX2, and COL1 genes at all tested concentrations, which fit well with the reduced alkaline phosphatase activity reported after 7 and 14 days of treatment. Reduced Col1 deposition in the extracellular matrix was reported after 14 days of treatment. Increased calcium deposits were observed in treated cells when compared to the control cultures. In conclusion, chronic exposure to 1 μM ZOL induced significant reduction of osteogenic differentiation, while ZOL concentrations ≥1.5 μM are required to impair PDLSCs viability and induce apoptosis.
Fraud in New Zealand, 1840–1939
Authors: Othman Radiah , Ameer Rashid , Laswad Fawzi
Published: October 2020

This study analyses fraudsters and their motives in New Zealand’s post-colonial times, using Papers Past, from 1840 to 1939. The aim is to understand the past societal context concerning fraud crimes. The historical analysis reveals that fraud was reported as early as 1840, and since then, reports of fraud have grown rapidly. False pretences and representation were the most common types of fraud, and there was a greater proportion of male than female perpetrators. Female criminality was the main subject of discussion during the period studied, and often biased perceptions regarding how typical women should behave were expressed. The motives of perpetrators of fraud were mainly to maintain cohesion for the family. Overall, the motivations for committing fraud indicated social struggles rather than greed. There is also some evidence for how fraudsters from privileged social classes received lighter sentencing regardless of the grave nature of the offence.
Attitudes of Front-Line Nurses Toward Hospice Care During the COVID-19 Pandemic
Authors: Liu Qinghua , Tao Jing , Gao Lianlian , He Xifei , Wang Yuping , Xia Wenlin , Huang Lihong
Published: October 2020

Objective:

The aim of this study was to understand the attitudes of front-line clinical nurses toward hospice care in the fight against the COVID-19 pandemic, to provide a source of reference for hospice care education and training in hospitals treating patients with COVID-19.

Method:

Front-line nurses from a designated COVID-19 hospital in Wuhan, China, participated. Participants completed the Chinese versions of the General Information Questionnaire, the Frommelt Attitudes Toward Care of the Dying Scale, the Jefferson Scale of Empathy, and the General Self-Efficacy Scale.

Results:

A total of 149 questionnaires were included in the analysis. The median total hospice care attitudes score was median 102.00(interquartile range, 95.5-120.50). The nurses’ attitudes toward hospice care were significantly associated with their age, knowledge of hospice care, level of empathy, and self-efficacy.

Conclusion:

The attitudes of front-line nurses toward hospice care need to be improved. Hospital departments should establish an effective public health emergency strategy, provide training to increase front-line nurses’ knowledge and practical experience of hospice care, cultivate nurses’ empathy, and enhance their sense of self-efficacy, in order to improve the quality of hospice care for patients and their families.
Predictive Validity of the Student Risk Screening Scale-Internalizing and Externalizing (SRSS-IE) Scores
Authors: Gregory Camara , Graybill Emily C. , Barger Brian , Roach Andrew T. , Lane Kathleen
Published: October 2020

Schools have become the most common setting for youth to receive additional supports—including access to mental health services serving as an entry point for identification and subsequent provision of these services. Many schools rely on office discipline referral (ODR) or suspension data as informal screeners for students who may need additional supports. Discipline data may be effective at identifying students with externalizing behaviors, yet students with internalizing behaviors may not engage in behaviors that warrant an ODR or suspension. As such, these students may go undetected in the absence of systematic screenings. This study explored whether a universal behavior screener identified students at risk for externalizing and internalizing concerns who may not have been identified through ODRs. The Student Risk Screening Scale for Internalizing/Externalizing (SRSS-IE) was completed for 1,201 elementary students in 3 elementary schools. SRSS-IE data were used to predict year-end ODRs. Results showed externalizing scores (SRSS-E7) on the SRSS-IE predicted year-end ODRs, with higher SRSS-E7 scores predicting with more ODRs. However, internalizing scores (SRSS-I5) on the SRSS-IE did not significantly predict year-end ODRs suggesting ODRs may not be an effective data source for identifying students with internalizing behaviors absent of externalizing behaviors.
Drug Repurposing in Neurological Disorders: Implications for Neurotherapy in Traumatic Brain Injury
Authors: Shakkour Zaynab , Habashy Karl John , Berro Moussa , Takkoush Samira , Abdelhady Samar , Koleilat Nadia , Eid Ali H. , Zibara Kazem , Obeid Makram , Shear Deborah , Mondello Stefania , Wang Kevin K. , Kobeissy Firas
Published: October 2020

Traumatic brain injury (TBI) remains a significant leading cause of death and disability among adults and children globally. To date, there are no Food and Drug Administration–approved drugs that can substantially attenuate the sequelae of TBI. The innumerable challenges faced by the conventional de novo discovery of new pharmacological agents led to the emergence of alternative paradigm, which is drug repurposing. Repurposing of existing drugs with well-characterized mechanisms of action and human safety profiles is believed to be a promising strategy for novel drug use. Compared to the conventional discovery pathways, drug repurposing is less costly, relatively rapid, and poses minimal risk of the adverse outcomes to study on participants. In recent years, drug repurposing has covered a wide range of neurodegenerative diseases and neurological disorders including brain injury. This review highlights the advances in drug repurposing and presents some of the promising candidate drugs for potential TBI treatment along with their possible mechanisms of neuroprotection. Edaravone, glyburide, ceftriaxone, levetiracetam, and progesterone have been selected due to their potential role as putative TBI neurotherapeutic agents. These drugs are Food and Drug Administration–approved for purposes other than brain injuries; however, preclinical and clinical studies have shown their efficacy in ameliorating the various detrimental outcomes of TBI.
Temozolomide-induced aplastic anaemia: Case report and review of the literature
Authors: Gilbar Peter J , Pokharel Khageshwor , Mangos Hilda M
Published: October 2020

Introduction

Temozolomide (TMZ) is an oral alkylating agent principally indicated for neurological malignancies including glioblastoma (GBM) and astrocytoma. Most common side effects are mild to moderate, and include fatigue, nausea, vomiting, thrombocytopenia and neutropenia. Severe or prolonged myelosuppression, causing delayed treatment or discontinuation, is uncommon. Major haematological adverse effects such as myelodysplastic syndrome or aplastic anaemia (AA) have rarely been reported.

Case report

We report a 68-year old female with GBM treated at a tertiary hospital with short-course radiotherapy and concurrent temozolomide following craniotomy. On treatment completion she was transferred to our hospital for rehabilitation. She was thrombocytopenic on admission. Platelets continued falling with significant pancytopenia developing over the next two weeks. Blood parameters and a markedly hypocellular bone marrow confirmed the diagnosis of very severe AA, probably due to TMZ.

Management and outcome

Treatment consisted of repeated platelet transfusions, intravenous antibiotics, antiviral and antifungal prophylaxis, and G-CSF 300 mcg daily. Platelet and neutrophil counts had returned to normal at 38 days following the completion of TMZ treatment.

Discussion

Whilst most cases of AA are idiopathic, a careful drug, occupational exposure and family history should be obtained, as acquired AA may result from viruses, chemical exposure, radiation and medications. Temozolomide-induced AA is well documented, though only 12 cases have been described in detail. Other potential causes were eliminated in our patient. Physicians should be aware of this rare and potentially fatal toxicity when prescribing. Frequent blood tests should be performed, during and following TMZ treatment, to enable early detection.
‘We Want Freedom Not Just Safety’: Biography of a Girlfesto as a Strategic Tool in Youth Activism
Authors: Gonick Marnina , Vanner Catherine , Mitchell Claudia , Dugal Anuradha
Published: October 2020

Girls are increasingly visible as activists demanding social change, equity and justice. Yet communication between girls and policymakers is fraught with challenges over how to translate young people’s knowledge into policy change. This article traces the history of the manifesto as a form for the marginalized to articulate new social visions, drawing on the Riot grrrl manifestos as examples of this genre. It describes the creation of the first Girlfesto at the 2018 Circles Within Circles event that brought together girls, young people, activists, researchers, and policymakers from six countries in the Global North and South to consider the role of community art-based activism by girls and young people in challenging gender-based violence, concentrating on colonial systems of violence against indigenous women and girls in Canada and South Africa. We analyse the Montebello Girlfesto and the opportunities and challenges in using the Girlfesto model, with reflections from girl participants.
Targeting the GRP78-Dependant SARS-CoV-2 Cell Entry by Peptides and Small Molecules
Authors: Allam Loubna , Ghrifi Fatima , Mohammed Hakmi , El Hafidi Naima , El Jaoudi Rachid , El Harti Jaouad , Lmimouni Badreddine , Belyamani Lahcen , Ibrahimi Azeddine
Published: October 2020

The global burden of infections and the rapid spread of viral diseases show the need for new approaches in the prevention and development of effective therapies. To this end, we aimed to explore novel inhibitor compounds that can stop replication or decrease the viral load of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which there is currently no approved treatment. Besides using the angiotensin-converting enzyme (ACE2) receptor as a main gate, the CoV-2 can bind to the glucose-regulating protein 78 (GRP78) receptor to get into the cells to start an infection. Here, we report potential inhibitors comprising small molecules and peptides that could interfere with the interaction of SARS-CoV-2 and its target cells by blocking the recognition of the GRP78 cellular receptor by the viral Spike protein. These inhibitors were discovered through an approach of in silico screening of available databases of bioactive peptides and polyphenolic compounds and the analysis of their docking modes. This process led to the selection of 9 compounds with optimal binding affinities to the target sites. The peptides (satpdb18674, satpdb18446, satpdb12488, satpdb14438, and satpdb28899) act on regions III and IV of the viral Spike protein and on its binding sites in GRP78. However, 4 polyphenols such as epigallocatechin gallate (EGCG), homoeriodictyol, isorhamnetin, and curcumin interact, in addition to the Spike protein and its binding sites in GRP78, with the ATPase domain of GRP78. Our work demonstrates that there are at least 2 approaches to block the spread of SARS-CoV-2 by preventing its fusion with the host cells via GRP78.
Coping self-efficacy influences health information avoidance
Authors: Hua Jacqueline , Howell Jennifer L.
Published: October 2020

Avoiding information about one’s health can have long-term implications for health and well-being. Two studies examined the relationship between health information avoidance and coping self-efficacy, or a sense that one can effectively cope. In Study 1, coping self-efficacy, but not general self-efficacy, was associated with information avoidance. In Study 2, participants who reflected on their positive coping strategies were less likely to avoid learning their risk for disease as compared to those who did not reflect on their coping strategies. These findings suggest that coping self-efficacy is a good target for future interventions aimed at reducing health information avoidance.
Vibration control of a military vehicle weapon platform under the influence of integrated ride and cornering dynamics
Authors: Banerjee Saayan , Balamurugan Varadarajan , Krishna Kumar R
Published: October 2020

The present study is focussed upon vibration control of the weapon elevation and azimuth dynamics mathematical model under influence of the 20 degree of freedom integrated ride and cornering dynamics non-linear mathematical model of a military vehicle with trailing arm suspension. The PID, LQR and Backstepping control techniques are applied in the weapon dynamics model in order to achieve the desired level of gun stabilisation over different manoeuvring situations. The control methods are initially implemented in the weapon elevation dynamics mathematical model which is subjected to vehicle ride response inputs only. In the later section, the control methods are incorporated in the azimuth dynamics mathematical model which is subjected to the integrated ride and cornering dynamic response inputs from different manoeuvres. The control forces are incorporated in the governing equations of motion for the weapon elevation and azimuth dynamics. The equations of motion for the weapon elevation and azimuth dynamics with incorporated control parameters, are transformed into state space matrix domain and solved by using Matlab. The elevation and azimuth dynamic responses are observed over the random terrain and with variable track velocities, respectively and compared with the corresponding passive models. It is observed that adequate attenuation of the vehicle dynamic inputs could be attained with the control techniques which led to desired weapon stabilisation in both elevation and azimuth directions. Compared to other controllers, backstepping control strategy is observed to provide the maximum attenuation in weapon responses by directly considering the Lyapunov stability criterion in its formulation. Therefore, the basic focus is to evaluate the gun muzzle response reduction by implementing different control techniques under the influence of a non-linear vehicle dynamic platform. These studies would provide the motivation and foundation behind design and development of gun control system for the military vehicles with All Electric Drive.
A critical discussion of the use of film in participatory research projects with homeless young people: an analysis based on case examples from England and Canada
Authors: Roy Alastair , Kennelly Jacqueline , Rowley Harriet , Larkins Cath
Published: October 2020

The focus of this paper is on the complex and sometimes contradictory effects of generating films with and about young people who have experienced homelessness, through participatory research. Drawing on two projects – one in Ottawa, Canada, and the other in Manchester, UK – we scrutinise two key aspects of participatory research projects that use film: first, how to appropriately communicate the complexity of already-stigmatised lives to different publics, and second, which publics we prioritise, and how this shapes the stories that are told. Through a theoretical framework that combines Pierre Bourdieu’s account of authorised language with Arthur Frank’s socio-narratology, we analyse the potential for generating justice versus reproducing symbolic violence through participatory research and film with homeless young people. In particular, we scrutinise the distinct role played by what we are calling first, second and third publics – each with their own level of distance and relationship to the participatory research process.
The Hebrew Bible and the ‘Animal Turn’
Authors: Sherman Phillip
Published: October 2020

Animal Studies refers to a set of questions which take seriously the reality of animal lives, past and present, and the ways in which human societies have conceived of those lives, related to them, and utilized them in the production of human cultures. Scholars of the Hebrew Bible are increasingly engaging animals in their interpretive work. Such engagement is often implicit or partial, but increasingly drawing directly on the more critical aspects of Animal Studies. This article proceeds as a tour through the menagerie of the biblical canon by exploring key texts in order to describe and analyze what Animal Studies has brought to the field of Biblical Studies. Biblical texts are grouped into the following categories: animals in the narrative accounts of the Torah, legal and ritual texts concerning animals, animal metaphors in the prophets, and wisdom literature and animal life. The emergence and application of zooarchaeological research and a number of studies focusing on specific animal species will be discussed. Sustained attention will be given to two recent works which have brought Animal Studies into the fractured fold of biblical scholarship more directly. Finally, I will suggest some future directions for the study of the Hebrew Bible in light of Animal Studies.
Ruth in Recent Research
Authors: Matheny Jennifer M.
Published: October 2020

From the early treatments focused on historical-critical methods to the interdisciplinary approaches of the social sciences today, Ruth research continues to speak to the current developments within interpretive conversations. This article briefly surveys major commentaries on Ruth, and then discusses the shifts in research from 2001 to today, highlighting future trajectories and trends.
Circ_0000215 Increases the Expression of CXCR2 and Promoted the Progression of Glioma Cells by Sponging miR-495-3p
Authors: Mutalifu Nurehemaiti , Du Peng , Zhang Jingjing , Akbar Halik , Yan Baofeng , Alimu Sulaiman , Tong Lingxiao , Luan Xinping
Published: October 2020

Background:

In recent years, accumulating studies have found that circular RNA (circRNA) exerts a great effect on tumor progression. Circ_0000215, a novel circRNA, remains largely unknown in terms of its effect and mechanism in glioma.

Method:

Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to detect the expressions of circ_0000215, miR-495-3p and CXCR2 in human glial cell line HEB and glioma cell lines (A172, U251, U87, SHG-44, LN-18), human glioma tissues and adjacent healthy tissues. Gain- and loss-assays of circ_0000215 were conducted. Cell proliferation ability was detected via the CCK8 assay, and cell invasion ability was examined by Transwell assay. CXCR2 expression was evaluated via RT-PCR and Western blot. Moreover, bioinformatics was applied to analyze the targeting molecules of circ_0000215 and CXCR2. Verification of the relationship between these molecules were supported through the dual-luciferase reporter gene and RNA immunocoprecipitation (RIP) assay.

Results:

Circ_0000215 and CXCR2 were remarkably upregulated in glioma tissues and cells. Overexpression of circ_0000215 notably promoted the proliferation, invasion and epithelial-mesenchymal transition (EMT) but inhibited apoptosis of glioma cells, while knocking down circ_0000215 had the opposite effects. Additionally, miR-495-3p, a sponge RNA of circ_0000215, inhibited the growth, invasion and EMT of glioma cells. Mechanistically, miR-495-3p targeted CXCR2 and negatively regulated CXCR2/PI3K/Akt pathway. However, the effects of miR-495-3p were all dampened by overexpression of circ_0000215.

Conclusion:

These data demonstrated that circ_0000215 functions as a competitive endogenous RNA by sponging miR-495-3p, thus accelerating glioma progression through CXCR2 axis.
Internal Lipid Profile and Body Lipid Profile in Relation to Cognition: A Cross-Sectional Study in Southern China
Authors: Liu Lian , Huang Xiao , Feng Liang , Wu Yanqing
Published: October 2020

Aim:

There are currently no established, clinically relevant, non-invasive markers of cognitive impairment, except for age and APOE genotype.

Methods:

A cross-sectional study of 1,296 participants from Nanchang, China, has been conducted. We collected data from Mini–Mental State Examination (MMSE) scores, internal lipid profiles and body lipid profiles, age and other factors that may have an effect on cognitive impairment.

Results:

Internal lipid profiles (OR = 1.03 [95%CI, 1.00-1.06], P = 0.024), body lipid profiles (OR = 1.05 [95%CI, 1.01-1.09], P = 0.014), and age (OR = 1.03 [95%CI, 1.01-1.05], P < 0.001) were all positively correlated with cognitive impairment.

Conclusions:

Cognitive impairment was more frequent in female patients with high internal lipid profiles or body lipid profiles, and these characteristics were related to age and education.
Preliminary six-month outcomes of LIMBSAVE (treatment of critical Limb IscheMia with infragenicular Bypass adopting in situ SAphenous VEin technique) registry
Authors: Troisi Nicola , Blasis Giovanni De , Salvini Mauro , Michelagnoli Stefano , Setacci Carlo ,   LIMBSAVE registry Collaborative Group
Published: October 2020

Objectives

Guidelines recommend open bypass surgery for long occlusions of infrainguinal arteries. In situ saphenous vein bypass is a standardized technique. The aim of this study was to report preliminary six-month outcomes of a national, multicenter, observational, prospective registry based on the examination of treatment of critical Limb IscheMia with infragenicular Bypass adopting the in situ SAphenous VEin technique (LIMBSAVE).

Methods

From January 2018 until October 2019, 428 patients from 41 centers were enrolled in the LIMBSAVE registry. Data were prospectively collected in a dedicated database, including demographics, preoperative risk factors, clinical and diagnostic preoperative assessments, intraoperative measures (including safety and effectiveness of the valvulotome during the surgical procedures), and 30-day follow-up data. Furthermore, estimated six-month outcomes according to Kaplan–Meier curves in terms of primary patency, primary assisted patency, secondary patency, and limb salvage were evaluated.

Results

Patients were predominantly male ( n = 332, 77.6%) with a mean age of 73.3 years (range 39–95). Technical success, defined as bypass pulse after use of the valvulotome, was obtained in all cases. The proximal anastomosis could be reached by the valvulotome in all cases. The mean number of valvulotome uses was 2.5 (range 1–5). No vein perforation was reported. In nine cases (2.1%), a vein lesion with intramural hemorrhage occurred. The mean length of hospital stay was 11.1 days (range 1–60). At 30-day follow-up, the overall bypass patency rate was 97.4%, and the rate of open or endo reinterventions for failing bypass was 5.4%. At six-month follow-up, the estimated primary patency, primary assisted patency, secondary patency, and limb salvage were 78.1%, 86.2%, 92.1%, and 94.7%, respectively.

Conclusions

Preliminary intraprocedural outcomes of the LIMBSAVE registry show that the in situ technique with the valvulotome is safe and effective in disrupting valves and obtaining pulsatility in the saphenous vein. The complication rate related to the use of the valvulotome is low. The six-month preliminary outcomes in terms of overall patency and limb salvage are promising. Further examinations and continuous follow-up are needed to evaluate long-term outcomes.
Uveal melanoma: progress in molecular biology and therapeutics
Authors: Li Yongyun , Shi Jiahao , Yang Jie , Ge Shengfang , Zhang Jianming , Jia Renbing , Fan Xianqun
Published: October 2020

Uveal melanoma (UM) is the most common intraocular malignancy in adults. So far, no systemic therapy or standard treatment exists to reduce the risk of metastasis and improve overall survival of patients. With the increased knowledge regarding the molecular pathways that underlie the oncogenesis of UM, it is expected that novel therapeutic approaches will be available to conquer this disease. This review provides a summary of the current knowledge of, and progress made in understanding, the pathogenesis, genetic mutations, epigenetics, and immunology of UM. With the advent of the omics era, multi-dimensional big data are publicly available, providing an innovation platform to develop effective targeted and personalized therapeutics for UM patients. Indeed, recently, a great number of therapies have been reported specifically for UM caused by oncogenic mutations, as well as other etiologies. In this review, special attention is directed to advancements in targeted therapies. In particular, we discuss the possibilities of targeting: GNAQ/GNA11, PLCβ, and CYSLTR2 mutants; regulators of G-protein signaling; the secondary messenger adenosine diphosphate (ADP)-ribosylation factor 6 (ARF6); downstream pathways, such as those involving mitogen-activated protein kinase/MEK/extracellular signal-related kinase, protein kinase C (PKC), phosphoinositide 3-kinase/Akt/mammalian target of rapamycin (mTOR), Trio/Rho/Rac/Yes-associated protein, and inactivated BAP1; and immune-checkpoint proteins cytotoxic T-lymphocyte antigen 4 and programmed cell-death protein 1/programmed cell-death ligand 1. Furthermore, we conducted a survey of completed and ongoing clinical trials applying targeted and immune therapies for UM. Although drug combination therapy based on the signaling pathways involved in UM has made great progress, targeted therapy is still an unmet medical need.
Agency and Structure in Latin American Regime Change
Authors: Ames Barry , Mamone Ignacio
Published: October 2020

Transitions from authoritarianism and breakdowns of democracy have long been central puzzles for scholars of Latin American politics. Because structural explanations have proved to be weak, recent work has emphasised political agency. This strand of research is promising, but major questions remain unanswered: Who are the key actors driving regime change? How do their individual preferences affect transitions and breakdowns? This article focuses on three central members of the political elite: presidents, opposition leaders, and military commanders. These actors develop unique preferences about regimes and unique degrees of radicalism regarding their preferred policies; in turn, these preferences and radicalism affect the probabilities of regime change. Testing the argument in 20 nations between 1945 and 2010, we find that an average measure of preferences masks crucial distinctions in the chain of regime change. Transitions to a competitive regime are more likely when autocrats have low intrinsic commitments to dictatorship. The survival of democracies hinges on whether top military officials develop pro-democratic preferences. The role of executive preferences, by contrast, is moderated by the attachments and radicalism of opposition leaders. Next, we examine how structural contexts shape both preferences and political outcomes, finding that economic development shapes both the emergence of preferences and radicalism and their impacts on regime change. Our findings improve the validity of political agency theories and reconcile the roles of actors with the environments in which regimes emerge and fall.
Predicting Diabetic Neuropathy Risk Level Using Artificial Neural Network and Clinical Parameters of Subjects With Diabetes
Authors: Dubey Venketesh N. , Dave Jugal M. , Beavis John , Coppini David V.
Published: October 2020

Background:

A risk assessment tool has been developed for automated estimation of level of neuropathy based on the clinical characteristics of patients. The smart tool is based on risk factors for diabetic neuropathy, which utilizes vibration perception threshold (VPT) and a set of clinical variables as potential predictors.

Methods:

Significant risk factors included age, height, weight, urine albumin-to-creatinine ratio, glycated hemoglobin, total cholesterol, and duration of diabetes. The continuous-scale VPT was recorded using a neurothesiometer and classified into three categories based on the clinical thresholds in volts (V): low risk (0-20.99 V), medium risk (21-30.99 V), and high risk (≥31 V).

Results:

The initial study had shown that by just using patient data ( n = 5088) an accuracy of 54% was achievable. Having established the effectiveness of the “classical” method, a special Neural Network based on a Proportional Odds Model was developed, which provided the highest level of prediction accuracy (>70%) using the simulated patient data ( n = 4158).

Conclusion:

In the absence of any assessment devices or trained personnel, it is possible to establish with reasonable accuracy a diagnosis of diabetic neuropathy by means of the clinical parameters of the patient alone.
Multigram Scale Synthesis and Anti-Influenza Activity of 3-Indoleacetonitrile Glucosides
Authors: Dong Jianghong , Yan Fengmei , Li Shuang , Li Zewen , Qin Yuanhang
Published: October 2020

Indole-3-acetonitrile-6- O-β-d-glucopyranoside 1 is a simple alkaloid with anti-influenza A virus activity extracted from Radix isatidis. Herein, a concise and practical total synthetic route of 1 was presented, starting from 6-benzyloxyindole on a multigram scale. The pivotal reaction sequence involved the Mannich reaction, the fluoride ion-induced elimination-addition reaction, and phase-transfer-catalyzed glycosylation reaction as key steps. In addition, compounds 1 and two modified derivatives 8 and 16 were tested for anti-influenza efficacy and cytotoxicity in vitro performed on Madin-Darby canine kidney cells. The results revealed that the compounds exerted antiviral activity against the influenza A virus to a certain extent and displayed no cytotoxicity. These findings could contribute to the development of traditional Chinese medicine R. isatidis for treating the influenza virus.
Biomechanical Comparison of a Novel Method of Tricortical Kirschner Wire Fixation of Distal Chevron Osteotomies of the First Metatarsal Versus Traditional Kirschner Wire and Versus Screw Fixation
Authors: Chen Jie , Black Natalie R. , Morris Randall , Panchbhavi Vinod K.
Published: October 2020

Introduction:

Traditional Kirschner wire (K-wire) stabilization of first metatarsal distal chevron osteotomy involves 1 cortex of fixation; however, unicortical fixation is associated with a high complication rate, including pin migration. A method of K-wire fixation utilizing 3 cortices may be biomechanically superior and potentially equivalent to single-screw fixation.

Methods:

Cadaveric specimens fixed with tricortical K-wires were tested in both the physiologic and cantilever conditions against specimens fixed with unicortical K-wires (N = 8) and single screws (N = 9) utilizing matched-pair comparison groups. Differences in physiologic and cantilever fixed/intact stiffness ratio and cantilever failure load were determined.

Results:

The tricortical fixation specimens had a significantly higher stiffness ratio in cantilever loading than the unicortical fixation specimens (60.50% tricortical, 34.17% unicortical, P = .02) but not in physiologic load (15.34% tricortical, 25.75% unicortical, P = .23). In cantilever failure loading, the tricortical fixation specimens had a significantly higher load to failure than the unicortical fixation specimens (132.81 N tricortical, 58.58 N unicortical, P < .01). Stiffness ratio under physiologic load, cantilever load, and ultimate load to failure were not significantly different between tricortical K-wire and screw-fixation groups.

Conclusion:

Tricortical K-wire fixation for distal chevron osteotomies is biomechanically superior to traditional unicortical K-wire fixation, and equivalent to single-screw fixation.

Levels of Evidence:

Level V: Cadaver study
Leaf Retention on Stem Cuttings of Two Zamia L. Species With or Without Anti-transpirants Does Not Improve Adventitious Root Formation
Authors: Deloso Benjamin E. , Paulino Charles J. , Marler Thomas E.
Published: October 2020

Improved horticultural practices may help to reduce demand for wild cycads threatened by unsustainable collection. We determined the influences of leaf retention with or without anti-transpirants on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings. Root formation success for both species was greater than 95%. The experimental treatments did not influence the percentage success or the speed of root development for Z. furfuracea or Z. integrifolia. The ending dry weights of the stems, leaves, and roots were also not influenced by the experimental treatments. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species was highly successful with or without retained leaves, and horticultural application of transpiration-reducing products on retained leaves did not improve success. Our findings add to the growing body of evidence that show how the horticulture nursery industry can meet horticultural demands in an effort to stop wild harvesting of threatened plants. Conservation of cycads as a group would benefit from more horticulture studies such as this, especially if the research includes threatened species.
Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity
Authors: Cruvinel Estela , Ogusuku Isabella , Cerioni Rosanna , Rodrigues Sirlene , Gonçalves Jéssica , Góes Maria Elisa , Alvim Juliana Morais , Silva Anderson Carlos , Lino Vanesca de Souza , Boccardo Enrique , Goulart Ernesto , Pereira Alexandre , Dariolli Rafael , Valadares Marcos , Biagi Diogo
Published: October 2020

Objectives:

To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency.

Methods:

Cells were kept in culture for over 50 passages, following a structured chronogram of passage and monitoring cell growth by population doubling time calculation and cell confluence. Standard procedures for human induced pluripotent stem cells monitoring as embryonic body formation, karyotyping and pluripotency markers expression were evaluated in order to assess the cellular state in long-term culture. Cells that underwent these tests were then subjected to differentiation into keratinocytes, cardiomyocytes and definitive endoderm to evaluate its differentiation capacity.

Results:

Human induced pluripotent stem cells clones maintained its pluripotent capability as well as chromosomal integrity and were able to generate derivatives from the three germ layers at high passages by embryoid body formation and high-efficient direct differentiation into keratinocytes, cardiomyocytes and definitive endoderm.

Conclusions:

Our findings support the routine of human induced pluripotent stem cells single-cell passaging as a reliable procedure even after long-term cultivation, providing healthy human induced pluripotent stem cells to be used in drug discovery, toxicity, and disease modeling as well as for therapeutic approaches.
Caval foramen hernia in a cat
Authors: Siow Jia Wen , Hoon QiCai Jason , Jenkins Elizabeth , Heblinski Nikola , Makara Mariano
Published: October 2020

Case summary

A 3-year-old neutered female domestic shorthair cat presented for a 2-week history of hyporexia, lethargy and weight loss. Aspartate aminotransferase, alanine aminotransferase and cholesterol were mildly elevated. Thoracic radiographs identified a lobulated soft tissue opacity in the caudal thorax to the right of midline, with the border effacing the caudal vena cava and broad-based towards the diaphragm. The broad base was suggestive of diaphragmatic hernia, with the other radiographic features and location suggestive of caval foramen hernia. Ultrasound confirmed diaphragmatic hernia with liver herniation. CT showed the herniation of multiple liver lobes and the gallbladder through a defect at the caval foramen. Herniorrhaphy was performed via ventral midline coeliotomy. Following this procedure, the cat’s clinical signs resolved and its weight has been regained.

Relevance and novel information

To our knowledge, this is the first report of successful caval foramen herniorrhaphy in a cat. Caval foramen hernia is a type of congenital diaphragmatic hernia. The authors suggest that its embryopathology involves defective septum transversum development. The case was detected during the standard diagnostic investigation of non-specific clinical signs. Its radiographic findings may easily be mistaken for a pulmonary mass. Although not seen in our case, caval foramen hernia is commonly associated with caudal vena cava obstruction, which can potentially result in Budd–Chiari-like syndrome.
Tweeting the #flushot: Beliefs, Barriers, and Threats During Different Periods of the 2018 to 2019 Flu Season
Authors: Guidry Jeanine P.D. , Austin Lucinda L. , O’Donnell Nicole H. , Coman Ioana A. , Lovari Alessandro , Messner Marcus
Published: October 2020

Influenza epidemics happen every year, with more than 8 million severe cases in 2017. The most effective way to prevent seasonal influenza is vaccination. In recent years, misinformation regarding vaccines abounds on social media, but the flu vaccine is relatively understudied in this area, and the current study is the first 1 to explore the content and nature of influenza information that is shared on Twitter, comparing tweets published in the early flu season with those posted in peak flu season. Using a quantitative content analysis, 1000 tweets from both parts of the flu season were analyzed for use of Health Belief Model (HBM) variables, engagement, and flu vaccine specific variables. Findings show several promising opportunities for health organizations and professionals: HBM constructs were present more frequently than in previous, related studies, and fewer vaccine-hesitant tweets appear to be present. However, the presence of high barriers to flu vaccine uptake increased significantly from early to peak season, including an increase in the mention of conspiracy theories. Flu vaccine related tweets appear to vary in misinformation level and density throughout the flu season. While this should be confirmed by further studies over multiple flu seasons, this a finding that should be considered by public health organizations when developing flu vaccine campaigns on social media.
COVID Care Clinic: A Unique Way for Family Medicine to Care for the Community During the SARS-CoV-2 (COVID-19) Pandemic
Authors: Jacobson Nathan A. , Nagaraju Darshan , Miller Jennifer M. , Bernard Matthew E.
Published: October 2020

The COVID-19 pandemic has presented new challenges in how Primary Care clinicians care for community patients. Our organization quickly allocated 1 of our community clinic sites into a dedicated COVID Clinic caring for the COVID positive or any patient with COVID like symptoms to minimize contact with the well patients. A prerequisite for all patients to be seen in the COVID Care Clinic was a virtual visit staffed with Advanced Practice Providers that would further determine if the patient needed to seek emergency medical care or be seen in the COVID Clinic. From March 23, 2020 through May 15, 2020, 852 patients with COVID symptoms were seen in this clinic rather than the emergency department. This article describes a collaborative effort to care for a community during the COVID-19 pandemic. This unique setting allowed us to focus an appropriate level of care to a high risk population in a safe and effective manner in the ongoing effort to flatten the epidemiological curve.
It Is Just a Rash They Said! Acute Skin Manifestation in a Patient With Vasculitis in Rural Hospitals
Authors: Sawalha Khalid , Kunnumpurath Anthony , Singh Shailendra , Kamoga Gilbert-Roy
Published: October 2020

A 76-year-old Caucasian male with a history of rheumatoid arthritis, Raynaud’s phenomenon, pulmonary embolism on warfarin, and a previous amputation of his left partial ring and fifth finger presented with acute onset of rash in bilateral lower extremities. He was recently started on trimethoprim-sulfamethoxazole due to concern for cellulitis. Differential diagnosis for acute-onset rash with the patient’s history presented as a challenge to the internist, as the differential is broad. Our case goes through the differential diagnosis to contrast the different presentations of rash in a patient with vasculitis. Ultimately skin biopsy in conjunction with a past positive cryoglobulinemic level helped confirm the diagnosis of cutaneous vasculitis, following which he was started on appropriate treatment and recovered.
Two Siblings With Valproate-Related Hyperammonemia and Novel Mutations in Glutamine Synthetase (GLUL) Treated With Carglumic Acid
Authors: Bennett Jennifer , Gilkes Christy , Klassen Karin , Kerr Marina , Khan Aneal
Published: October 2020

This case report describes 2 siblings with myoclonic epilepsy who had novel mutations in the glutamine synthetase ( GLUL) gene: c.316C>T, p.(Arg106*) and c.42G>C, p.(Lys14Asn). Valproic acid improved seizure control, but was associated with hyperammonemic encephalopathy. Addition of carglumic acid reduced ammonia levels but drug coverage was declined. We therefore designed a protocol to measure the reduction in plasma ammonia in response to carglumic acid therapy. After the first dose of carglumic acid, Patient 1 showed a reduction in plasma ammonia levels within 3 hours, from 114 umol/L to 68 umol/L (reference 12-47 umol/L), and Patient 2 from 108 umol/L to 80 umol/L, which was sustained over a 2 week period. Overall, there was a strong negative correlation between plasma ammonia levels and carglumic acid levels (r = −0.86, p = 0.0013), and recurrence of hyperammonemic encephalopathy was not observed while the patients were taking carglumic acid.
Addressing Service Recovery Practice With Radiation Oncology Frontline Managers: A Project Brief
Authors: Washington Charles , Benvengo Stephanie , Lynch Kathleen
Published: October 2020

The relationship between patient satisfaction, care compliance, and treatment outcomes suggests patients who report dissatisfaction perceive receipt of suboptimal care. Patient satisfaction plays a role in defining quality of care, affecting institutional reimbursement and reputation capital. Using an explanatory sequential mixed methodology approach, this study explored frontline management’s role in effective service recovery, actively addressing instances of patient dissatisfaction to improve the overall patient experience. A survey of frontline managers, document and artifact reviews, and probing interviews identify the importance of consistent performance measurement, feedback, and frequent leadership training on the relevance and importance of service recovery.
Industrial dynamics on the commodity frontier: Managing time, space and form in mining, tree plantations and intensive aquaculture
Authors: Banoub Daniel , Bridge Gavin , Bustos Beatriz , Ertör Irmak , González-Hidalgo Marien , de los Reyes Julie Ann
Published: October 2020

Research in political ecology and agrarian political economy has shown how commodity frontiers are constituted through the appropriation and transformation of nature. This work identifies two broad processes of socio-metabolism associated with commodity frontiers: the spatial extension of nature appropriation, via expanding territorial claims to the control and use of natural resources and associated acts of dispossession (commodity-widening); and the intensification of appropriation at existing sites, through socio-technical innovation and the growing capitalisation of production (commodity-deepening). While sympathetic, we have reservations about reducing frontier metabolism to either one or the other of these processes. We argue for more grounded examinations of how non-human nature is actively reconstituted at commodity frontiers, attuned to the diverse and specific ways in which socio-ecological processes are harnessed to dynamics of accumulation. To achieve this, we compare strategies of appropriation in three sectors often associated with the commodity frontier: gold mining, tree plantations and intensive aquaculture. In doing so, we bring research on capitalism as an ecological regime into conversation with work on the industrial dynamics of ‘nature-facing’ sectors. By harnessing the analytical categories of time, space and form adopted by research on industrial dynamics, we (i) show how strategies of commodity-widening and commodity-deepening are shaped in significant ways by the biophysical characteristics of these sectors; and (ii) identify a third strategy, beyond commodity-widening and commodity-deepening, that involves the active reconstitution of socio-ecological systems – we term this ‘commodity-transformation’.