bluuebunny/crossref_metadata_2025 huggingface dataset:
Computational Investigation of the Hemodynamic Effects of the Location of a Re-Entry Tear in Uncomplicated Type B Aortic Dissection
Authors: Kim Eunji , Chung Sung Woon , Huh Up , Song Seunghwan , Lee Chung Won , Wang Il Jae , Song Chanhee , Goh Tae Sik , Park Jong-Hwan , Ryu Dongman
Published: October 2024

This study aimed to examine the hemodynamic modifications in uncomplicated type B aortic dissection in relation to the location of re-entry tears using a computational fluid dynamics simulation. The geometry of uncomplicated type B aortic dissection was reconstructed using computed tomography images. Subsequently, 10 virtual models were artificially generated with re-entry tears at various locations. The simulation results indicated that most models with re-entry tears had lower pressure and wall shear stress than those without re-entry tears. The overall pressure distribution of the true lumen was greater than that of the models without re-entry tears when the re-entry tear was placed at the end of the false lumen. Furthermore, the recirculation phenomenon in the false lumen was reduced as the re-entry tear was relocated to the distal region of the aorta. To determine whether and how to perform fenestration surgery in patients with uncomplicated type B aortic dissection, these computational results can be used as supplemental indicators. However, further validation in a larger number of patients through additional investigation is necessary.
Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against Enterococcus faecalis Using CFU, RT-PCR, and SEM
Authors: Jovanović Radovan , Ilić Jugoslav , Šubarić Ljiljana , Vlahović Zoran , Simić Sanja , Arsić Zoran , Radunović Milena , Popović Branka
Published: October 2024

Eradication of microorganisms present in the root canal system during endodontic therapy is one of the critical factors affecting the final outcome of endodontic treatment. However, even adequate technique of the root canal treatment and use of irrigants according to the established protocol does not lead to the complete elimination of microorganisms during endodontic treatment. The presence of Enterococcus (E.) faecalis inside the root canal system may be an important factor contributing to the failure of the treatment. Introducing agitation techniques in irrigation, such as passive ultrasonic irrigation (PUI) and the use of the activating instrument XP-endo Finisher (XPF), contribute to a better debridement and disinfection of the root canal. This study was conducted on 42 root canals experimentally inoculated with E. faecalis and exposed to three irrigation protocols. These have included a standard irrigation protocol using a syringe and needle, passive ultrasonic irrigation, and activation of the irrigant using an XPF instrument. The reduction in microorganisms was evaluated using the quantitative polymerase chain reaction in real-time (RT-PCR) as well as via the method of determining the number of colony forming units on nutrient medium (CFUs). The results of this study showed that the use of supplementary irrigation protocols, PUI, and XPF led to a significant decrease in the number of microorganisms inside the root canal of experimental teeth. These findings indicate a significant positive impact of these procedures on the disinfection of the root canal system.
BDNF Differentially Affects Low- and High-Frequency Neurons in a Primary Nucleus of the Chicken Auditory Brainstem
Authors: McLellan Kristine , Sabbagh Sima , Takahashi Momoko , Hong Hui , Wang Yuan , Sanchez Jason Tait
Published: October 2024

Neurotrophins are proteins that mediate neuronal development using spatiotemporal signaling gradients. The chicken nucleus magnocellularis (NM), an analogous structure to the mammalian anteroventral cochlear nucleus, provides a model system in which signaling between the brain-derived neurotrophic factor (BDNF) and tyrosine receptor kinase B (TrkB) is temporally regulated. In the NM, TrkB expression is high early in development (embryonic [E] day 9) and is downregulated until maturity (E18–21). It is currently unknown how BDNF–TrkB signaling affects neuronal properties throughout development and across a spatial (i.e., frequency) axis. To investigate this, we exogenously applied BDNF onto NM neurons ex vivo and studied intrinsic properties using whole-cell patch clamp electrophysiology. Early in development (E13), when TrkB expression is detectable with immunohistochemistry, BDNF application slowed the firing of high-frequency NM neurons, resembling an immature phenotype. Current measurements and biophysical modeling revealed that this was mediated by a decreased conductance of the voltage-dependent potassium channels. Interestingly, this effect was seen only in high-frequency neurons and not in low-frequency neurons. BDNF–TrkB signaling induced minimal changes in late-developing NM neurons (E20–21) of high and low frequencies. Our results indicate that normal developmental downregulation of BDNF–TrkB signaling promotes neuronal maturation tonotopically in the auditory brainstem, encouraging the appropriate development of neuronal properties.
Comparison of Physiological Stress Indices in Anesthetized and Manually Restrained Leopard Sharks, Triakis semifasciata
Authors: Holst Meghan M. , Wheaton Catharine J. , Schoen Alexandra N. , Spangenberg Jill V. , McEligot Kevin T. , Schouest Melissa L. , Burns Charlene M. , Mylniczenko Natalie D.
Published: October 2024

Background: Leopard sharks (Triakis semifasciata) are abundant, coastal, eastern Pacific, mesopredatory sharks and are frequently managed in aquariums and zoos. Medical examinations are a routine part of good husbandry practices, but the handling protocols vary greatly between facilities. In this study, we compared the physiological stress responses of T. semifasciata associated with manual restraint and sedated handling under parallel holding and handling procedures in a 10 min interval. Methods: Blood was collected and analyzed for the hematocrit (Hct), lactate, glucose, beta-hydroxybutyrate (β-HB), pH, blood gasses, 1α-hydroxycorticosterone (1α-OHB), and corticosterone (B). Results: Overall, a minimal physiological stress response was observed in both groups. However, statistically significant increases in the PCO2, β-HB and lactate occurred, while the pHTC, SO2, and BE decreased over time in the manual-restraint group. In the sedated group, the B, PCO2, and lactate increased while the pHTC, SO2, and base excess (BE) decreased over time. When comparing treatments, the sedated group had a higher B and PCO2 TC and a lower pHTC, BE, and SO2 than the manual-restraint group at the second time point. Conclusions: This study indicates that manual restraint is comparable to the described sedation in T. semifasciata for minimally invasive procedures. The overall results show that this species had low physiological stress responses to both methods.
Structural Insights into the Iodothyronine Deiodinase 2 Catalytic Core and Deiodinase Catalysis and Dimerization
Authors: Towell Holly , Braun Doreen , Brol Alexander , di Fonzo Andrea , Rijntjes Eddy , Köhrle Josef , Schweizer Ulrich , Steegborn Clemens
Published: October 2024

Iodothyronine deiodinases (Dio) are selenocysteine-containing membrane enzymes that activate and inactivate the thyroid hormones (TH) through reductive iodide eliminations. The three deiodinase isoforms are homodimers sharing highly conserved amino acid sequences, but they differ in their regioselectivities for the deiodination reaction and regulatory features. We have now solved a crystal structure of the mouse deiodinase 2 (Dio2) catalytic domain. It reveals a high overall similarity to the deiodinase 3 structure, supporting the proposed common mechanism, but also Dio2-specific features, likely mediating its unique properties. Activity studies with an artificially enforced Dio dimer further confirm that dimerization is required for activity and requires both the catalytic core and the enzyme’s N-terminus. Cross-linking studies reveal the catalytic core’s dimerization interface, providing insights into the architecture of the complete, active Dio homodimer.
LSD1 Demethylates and Destabilizes Autophagy Protein LC3B in Ovarian Cancer
Authors: Li Mingyang , Feng Jie , Zhao Kangrong , Huang Ting , Zhang Bowen , Yang Yifan , Sun Aiqin , Lin Qiong , Shao Genbao
Published: October 2024

Autophagy is a complex cellular process that can either promote or inhibit cancer progression and development, depending on the context and molecular regulation involved. This study investigates how LSD1 regulates autophagy in ovarian cancer by interacting with the autophagy protein LC3B. Utilizing the bioinformatic analysis of TCGA, CPTAC, and GEO datasets, as well as immunohistochemistry in ovarian cancer patients, we explored the expression association between LSD1 and LC3B. Molecular mechanisms were further analyzed using Western blotting, immunoprecipitation, and GST pull-down assays. Our findings reveal that LSD1 binds to LC3B via its SWIRM domain, and high levels of LSD1 are closely associated with aggressive ovarian cancer and poor patient outcomes. Mechanistically, LSD1 demethylates LC3B, leading to decreased LC3B stability. The observed inverse correlation between LSD1 expression and LC3B protein levels in clinical samples underscores the need for further investigation to elucidate how reduced LC3B protein levels induced by LSD1 demethylation may contribute to ovarian cancer.
Dexamethasone’s Clinical Efficacy in Experimental Autoimmune Pancreatitis Correlates with a Unique Transcriptomic Signature, Whilst Kinase Inhibitors Are Not Effective
Authors: Agrifoglio Ottavia , Kasprick Anika , Gross Natalie , Wahlig Marc , Kauffold Emilia , Woitas Aline , Vorobyev Artem , Ehlers Luise , Ludwig Ralf J. , Bieber Katja , Jaster Robert
Published: October 2024

(1) Background: Autoimmune pancreatitis (AIP) is mainly treated with steroids. Using an AIP mouse model, we investigated two potential alternatives, the transforming growth factor-β-activated kinase 1 inhibitor, takinib, and the Janus kinase inhibitor, tofacitinib. (2) Methods: In a multicenter preclinical study, MRL/MpJ mice were injected with polyinosinic/polycytidylic acid (poly I:C) for two weeks to induce AIP. They were then treated for four weeks with either takinib (25, 50, or 75 mg/kg body weight), tofacitinib (5, 10 or 15 mg/kg), dexamethasone (1 mg/kg), or solvent, while the poly I:C injections were continued. The severity of AIP was assessed histopathologically. Flow cytometry was used to examine lymphocyte subtypes in the spleen and mesenteric lymph nodes. The pancreatic gene expression profiles were analyzed by RNA sequencing. (3) Results: Poly I:C-treated mice developed severe AIP with inflammation, destruction of acinar tissue, and fibrosis. Dexamethasone significantly attenuated the disease, while takinib or tofacitinib had no effects. Dexamethasone also antagonized the effects of poly I:C on the relative frequencies of the AIP-associated lymphocyte subtypes CD4/CD69, CD8/CD44high, and CD4/CD25/FoxP3 in the spleen. In the principal component analysis of pancreatic transcriptomics, poly I:C-injected mice treated with tofacitinib, takinib, or solvent clustered together, while untreated and dexamethasone-treated mice formed separate, unique clusters. (4) Conclusions: Dexamethasone effectively reduced AIP severity, while takinib and tofacitinib were ineffective. The unique gene expression profile in dexamethasone-treated mice may provide a basis for identifying new drug targets for AIP treatment.
Amino Acid Deprivation in Glioblastoma: The Role in Survival and the Tumour Microenvironment—A Narrative Review
Authors: Du Keven , Grocott Leila , Anichini Giulio , O’Neill Kevin , Syed Nelofer
Published: October 2024

Background: Glioblastoma is the most common and aggressive primary brain tumour, characterised by its invasive nature and complex metabolic profile. Emerging research highlights the role of amino acids (AAs) in glioblastoma metabolism, influencing tumour growth and the surrounding microenvironment. Methods: This narrative review synthesises recent pre-clinical studies focusing on the metabolic functions of AAs in glioblastoma. Key areas include the effects of AA deprivation on tumour growth, adaptive mechanisms, and the tumour microenvironment. Results: The effects related to arginine, glutamine, methionine, and cysteine deprivation have been more extensively reported. Arginine deprivation in arginine-auxotrophic glioblastomas induces apoptosis and affects cell adhesion, while glutamine deprivation disrupts metabolic pathways and enhances autophagy. Methionine and cysteine deprivation impact lipid metabolism and ferroptosis. Tumour adaptive mechanisms present challenges, and potential compensatory responses have been identified. The response of the microenvironment to AA deprivation, including immune modulation, is critical to determining therapeutic outcomes. Conclusions: Targeting AA metabolism offers a promising approach for glioblastoma treatment, with potential targeted drugs showing clinical promise. However, the complexity of tumour adaptive mechanisms and their impact on the microenvironment necessitates further research to optimise combination therapies and improve therapeutic efficacy.
Endoscopic Sinus Surgery Significantly Reduces Eustachian Tube Dysfunction Symptoms in Patients with Chronic Rhinosinusitis: A Systematic Review and Meta-Analysis
Authors: Yang Kai-Shan , Chen Wei-Chih , Wu Ching-Nung , Wee Yin-Shen , Wang Ching-Shuen , Wu Cheng-Chih , Luo Sheng-Dean
Published: October 2024

(1) Background: Eustachian tube dysfunction (ETD) is a frequently observed clinical manifestation of chronic rhinosinusitis (CRS). This systematic review aimed to evaluate the function of the Eustachian tube following endoscopic sinus surgery (ESS) in adult CRS patients with confirmed preoperative ETD symptoms. (2) Methods: A systematic search of PubMed, Cochrane, Embase, and MEDLINE electronic databases was conducted. The review was performed following the PRISMA guidelines. Studies investigating concurrent ETD in CRS patients who underwent ESS were retrieved. The changes in ETD outcomes were measured by the 7-item Eustachian Tube Dysfunction Questionnaire (ETDQ-7). We employed a random-effects model to conduct the meta-analysis (3) Results: We included seven observational studies that involved a total of 436 CRS patients with concurrent ETD. The pooled results revealed a statistically significant reduction (Standardized mean difference = −1.24; 95% CI = −1.64 to −0.84) in ETDQ-7 scores among the CRS with ETD patient cohort at the 3-month postoperative follow-up. (4) Conclusions: ESS serves as an effective intervention for improving E-tube function in adult CRS patients with concurrent ETD. Future prospective randomized controlled trials that incorporate various outcome predictors should be conducted to explore potential clinical factors for greater ETD improvement and normalization after ESS.
Autistic Sensory Traits and Psychological Distress: Mediating Role of Worry and Intolerance of Uncertainty
Authors: Recio Patricia , Pozo Pilar , García-López Cristina , Sarriá Encarnación
Published: October 2024

Background/Objectives: Autistic adults exhibit core and comorbid features that can have an impact on their daily functioning and lead to considerable psychological distress. Significant and consistent associations have been identified between autism characteristics—restricted repetitive behaviors and sensory features—and high levels of stress and anxiety. For a better understanding of the issue, it is necessary to consider the cognitive aspects that can help explain variations in stress and anxiety levels among adults with autism. We therefore aimed to model the contributions of worry and intolerance of uncertainty on the relationship between autism characteristics (sensory sensitivities and repetitive behaviors) and psychological distress (stress and anxiety). Methods: The sample comprised 144 autistic individuals with a mean age of 35.15 (SD = 11.44). They participated in the study by completing an online questionnaire to assess the study variables. Most of the participants reported being diagnosed with Asperger syndrome (63.6%) or Level 1 autism spectrum disorder (27.1%). Results: The model fit index values tested through path analysis indicated a good fit (χ2 = 5.65; p = 0.13 ns; CFI = 0.993; NFI = 0.985; RMSEA = 0.079; SRMR = 0.025) and identified worry and intolerance of uncertainty as significant mediating variables within a comprehensive explanatory model. Conclusions: These findings highlight the importance of worry and intolerance of uncertainty as specific targets in interventions aimed at improving stress and anxiety problems in autistic adults.
Public Policies for the Energy Efficiency of Buildings in Mexico
Authors: Castro-Bello Mirna , Gómez-Muñoz Lizbeth , Marmolejo-Vega Carlos Virgilio , Morales-Morales Cornelio , Valencia-Díaz Eleazar Felipe , Maldonado-Catalán José Filiberto , Marmolejo-Duarte Carlos
Published: October 2024

In Latin America, the energy crisis has worsened due to the dependence on energy services and fossil fuel imports from highly industrialized countries at prices established by the international market; this is particularly relevant to the construction industry, which presents a significant deficit in optimal energy consumption. Hence, some governments have established public policies to maximize the efficiency of these services and, at the same time, minimize the carbon footprint. In this research study, we reviewed the public policies, strategies, and incentives for energy efficiency (EE) implementation in the residential sector established by the Mexican government. A scoping review methodology was chosen and implemented in the following steps: 1. Research inquiry identification. 2. Determination of the relevant literature and studies. 3. The literature selection. 4. Data graphing. 5. Results collection, overview, and submission. In this systematic review, we identified five mandatory standards (NOM-008-ENER-2001, NOM-009-ENER-2014, NOM-018-ENER-2011, NOM-020-ENER-2011, and NOM-024-ENER-2012), six optional standards, four strategies (Green Mortgage, Integral Sustainable Improvement in Existing Housing, ECOCASA, and NAMA), and three kinds of incentives (green bonds, credit and interest rates (Green Mortgage, FIDE, and Ecocasa), and taxes (Income Tax Reduction)). As a result of the implementation of the above, as of December 2020, NAMA financed 5106 developers of 38 projects in 15 states; contributed to a reduction of 126,779 tons of CO2; and aided 19,913 people. From 2013 to December 2023, EcoCasa subsidized 71,440 households for a total of 224 projects in 25 states; contributed to a reduction of 2.6 million tons of CO2; aided 285,760 Mexicans; and issued EcoCasa certificates for 3,473,556 m2. The results of the EE indicators in residential buildings showed an increase in the housing unit number as well as an increase in household appliances, with those based on power consumption prevailing. The residential sector ranks third in power consumption in Mexico, consuming an estimated 790 pj, of which 76% corresponds to thermal energy and 24% to electric power. Among countries in Latin America and the Caribbean, Mexico has achieved an Energy Transition Index of 62%.
Multi-Domain Environmental Quality of Indoor Mixed-Use Open Spaces and Insights into Healthy Living—A Quarantine Hotel Case Study
Authors: Wang Han , Pan Wenjian
Published: October 2024

In the post-pandemic context, data-driven design interventions that can endow architectural spaces with mixed-use and open characteristics that are adaptable and environmentally resilient are increasingly important. Ubiquitous semi-public architecture, such as hotel buildings, plays a crucial role in public health emergencies. Many hotels adopt mixed-use and open room spatial layouts, integrating diverse daily functions into a single tiny space, fostering flexible utilization and micro-scale space sharing; however, these also introduce potential health risks. This study offers a comprehensive evaluation of the indoor environmental quality (IEQ) of a hotel room space and discusses feasible intervention strategies for healthier renovation and rehabilitation. Taking a hotel in Shenzhen as a case, a multi-domain environmental assessment was conducted during the COVID-19 quarantine period in the summer of 2022. The study examines the health risks inherent in the hotel’s guest room and the varying patterns of IEQ factors across the hotel’s domains, including volatile organic compound concentrations, physical environmental parameters, and heat stress indices. The results illustrate diverse change trends in the chemical, physical, and heat stress factors present in the tested quarantined hotel room space throughout a typical summer day. Although most of the examined environmental factors meet local and global standards, some problems draw attention. In particular, the PM2.5 concentration was generally observed to be above the World Health Organization (WHO) air quality guideline (AQG) standards, and the interior lighting did not meet required standards most of the time. Moreover, correlation and multiple regression analyses uncover significant influence by physical environmental conditions on the concentrations of chemical pollutants in the hotel room. The study preliminarily identifies that higher relative humidity could lead to a lower concentration of CO2 while a higher PM2.5 concentration. Wet bulb globe temperature (WBGT) was observed to positively affect CO2 concentration. Further, the results suggest that even with relatively rigorous initial adjustment and re-renovation, multi-domain environmental quality in air-conditioned quarantine hotel rooms should be monitored and ameliorated from time to time. Overall, this study offers a scientific foundation for healthier upgrades of existing hotel buildings as well as provides insights into achieving environmental resilience in newly constructed hotel buildings for the post-pandemic era.
From Real-World Data to Causally Interpretable Models: A Bayesian Network to Predict Cardiovascular Diseases in Adolescents and Young Adults with Breast Cancer
Authors: Bernasconi Alice , Zanga Alessio , Lucas Peter J. F. , Scutari Marco , Di Cosimo Serena , De Santis Maria Carmen , La Rocca Eliana , Baili Paolo , Cavallo Ilaria , Verderio Paolo , Ciniselli Chiara M. , Pizzamiglio Sara , Blanda Adriana , Perego Paola , Vallerio Paola , Stella Fabio , Trama Annalisa ,   The Ada Working Group
Published: October 2024

Background: In the last decades, the increasing number of adolescent and young adult (AYA) survivors of breast cancer (BC) has highlighted the cardiotoxic role of cancer therapies, making cardiovascular diseases (CVDs) among the most frequent, although rare, long-term sequalae. Leveraging innovative artificial intelligence (AI) tools and real-world data (RWD), we aimed to develop a causally interpretable model to identify young BC survivors at risk of developing CVDs. Methods: We designed and trained a Bayesian network (BN), an AI model, making use of expert knowledge and data from population-based (1036 patients) and clinical (339 patient) cohorts of female AYA (i.e., aged 18 to 39 years) 1-year survivors of BC, diagnosed in 2009–2019. The performance achieved by the BN model was validated against standard classification metrics, and two clinical applications were proposed. Results: The model showed a very good classification performance and a clear causal semantic. According to the predictions made by the model, focusing on the 25% of AYA BC survivors at higher risk of developing CVDs, we could identify 81% of the patients who would actually develop it. Moreover, a desktop-based app was implemented to calculate the individual patient’s risk. Conclusions: In this study, we developed the first causal model for predicting the CVD risk in AYA survivors of BC, also proposing an innovative AI approach that could be useful for all researchers dealing with RWD. The model could be pivotal for clinicians who aim to plan personalized follow-up strategies for AYA BC survivors.
The Effect of a Tailored Educational Flyer on Colorectal Cancer Screening Among Rural Residents: Lessons Learned from a Pilot Randomized Trial
Authors: Kim Jungyoon , Beseler Cheryl , Leypoldt Melissa , Subramanian Roma , Robinson Tamara , Funkenbusch Karen , Foster Jason , Harris Susan , Yoder Aaron , Hymel Emma , Watanabe-Galloway Shinobu
Published: October 2024

Background/Objectives: Stool-based tests, such as the fecal immunochemical test (FIT), have been widely used for increasing colorectal cancer (CRC) screening. Small media, such as printed materials or flyers, are known to be an effective intervention to increase CRC screening by fecal tests. However, more evidence is needed to determine whether such small media are effective in improving screening uptake of a mailed FIT intervention targeted at rural populations in the USA. Methods: In this randomized study, 1230 FIT kits were mailed from July to December 2022 to rural Nebraskans aged 45–74 who were not up to date on CRC screening. Half of the participants (n = 608) also received a tailored, one-page, gender-specific educational flyer created based on focus groups with rural residents. Logistic regression was used to determine predictors of returning the FIT. Results: Study participants were predominantly female (76%), non-Hispanic White (83%), and within the age group of 55–64 (43%). Overall, 192 (15.6%) kits were returned (16.1% from the flyer group; 15.1% from the no-flyer group). However, we found no significant differences between the flyer and no-flyer groups (Adjusted Odds Ratio [AOR]: 1.21; 95% CI: 0.88–1.66). Females (AOR: 1.78; 95% CI: 1.19–6.14) and the oldest (65–74) age group (AOR: 5.03; 95% CI: 2.78–8.47) were more likely to return FIT kits than males and the youngest (45–54) age group. Conclusions: A tailored educational flyer was not effective in improving the CRC-screening-rate-by-mailed-FIT approach for rural populations. Future research should explore the content, timing, and mode of delivery of educational interventions as well as other multi-component strategies to improve screening rates. Public health officials might also consider developing strategies targeted at males and younger (45–54) age groups.
Circulating Neoplastic-Immune Hybrid Cells Are Biomarkers of Occult Metastasis and Treatment Response in Pancreatic Cancer
Authors: Patel Ranish K. , Parappilly Michael , Farley Hannah C. , Latour Emile J. , Wang Lei G. , Nair Ashvin M. , Lu Ethan S. , Sims Zachary , Park Byung , Nelson Katherine , Mayo Skye C. , Mills Gordon B. , Sheppard Brett C. , Chang Young Hwan , Gibbs Summer L. , Kardosh Adel , Lopez Charles D. , Wong Melissa H.
Published: October 2024

Background/Objectives: Pancreatic ductal adenocarcinoma (PDAC) presents significant diagnostic and prognostic challenges, as current biomarkers frequently fail to accurately stage disease, predict rapid metastatic recurrence (rPDAC), or assess response to neoadjuvant therapy (NAT). We investigated the potential for circulating neoplastic-immune hybrid cells (CHCs) as a non-invasive, multifunctional biomarker for PDAC. Methods: Peripheral blood specimens were obtained from patients diagnosed with PDAC. CHCs were detected by co-expression of pan-cytokeratin and CD45, normalized to 50,000 peripheral blood mononuclear cells. rPDAC was defined as metastatic recurrence within six months of margin-negative pancreatectomy. Cyclic immunofluorescence (CyCIF) analyses compared hybrid phenotypes in blood and tumors. Results: Blood samples were collected from 42 patients with PDAC prior to resection. Those with radiographically occult metastatic disease and rPDAC had higher preoperative CHC numbers compared to patients who did not (65.0 and 74.4, vs. 11.52 CHCs; p < 0.001). Patients with complete or near-complete pathologic responses to NAT had lower preoperative CHC numbers than partial and/or non-responders (1.7 vs. 13.1 CHCs; p = 0.008). When assessed longitudinally, those with partial pathologic response saw CHC levels become undetectable while on treatment but increase in the interval between NAT completion and resection. In contrast, patients with poor responses or development of metastatic disease experienced persistent CHC detection during therapy or rising levels prior to radiographic evidence of metastases. Further, in metastatic PDAC patients, treatment-induced phenotypic changes in hybrid cells mirrored those in paired metastatic tumor samples. Conclusions: CHC enumeration and phenotyping display promise as a real-time indicator of disease burden, recurrence risk, and treatment response in PDAC. CHCs have great potential as tumor-derived biomarkers to optimize therapeutic strategies and improve survival in patients with PDAC.
Immobilization of Phospholipase D on Magnetic Graphene Oxide for Efficient Phosphatidylserine Production
Authors: Shang Huiyi , Guo Bishan , Wang Juntan , Li Huijuan , Zhu Haihua
Published: October 2024

Phosphatidylserine (PS) has significant applications in various sectors, such as the medical and food industries. However, its production relies heavily on phospholipase D (PLD), a crucial tool that is hindered by issues like poor stability and irrecoverability. Immobilization presents itself as an effective solution to overcome these limitations. In this study, magnetic graphene oxide (MGO) modified with an amino (NH2) group was synthesized and utilized for PLD immobilization. The activity of the immobilized PLD (MGO-PLD) reached 3062 U/gMGO, with a specific activity of 33.9 U/mgPLD, virtually identical to that of the free PLD. MGO-PLD was utilized to synthesize PS efficiently in a biphasic system. Under optimal conditions, the PS yield reached 18.66 g/L, with a conversion rate of 92.8% and a productivity of 3.11 g/L/h. Notably, MGO-PLD retained an impressive PS conversion rate of 77.4% even after seven repetitive usages. Moreover, MGO-PLD displayed enhanced thermal and pH resistance properties compared to free PLD, alongside augmented storage stability. After an 8-week preservation at 4 °C, its residual activity was maintained at 76.3%. This study provides a sustainable and highly efficient pathway for the biocatalytic synthesis of PS.
The Role of Early Child Nutrition in Pulmonary Hypertension—A Narrative Review
Authors: Luca Alina-Costina , Stoica Cristina , Diaconescu Cosmin , Țarcă Elena , Roșu Solange Tamara , Butnariu Lăcrămioara Ionela , Stana Bogdan Aurelian , Gafton Bogdan , Curici Antoanela , Roșu Eduard Vasile , Mîndru Dana Elena
Published: October 2024

Pulmonary hypertension is a complex condition that has distinct characteristics in pediatric populations. This review explores the important role of early childhood nutrition in the growth, progression, and management of pediatric pulmonary hypertension. Nutritional deficiencies, including those of vitamins C, D, and iron, are closely linked to worse outcomes in children with this disease, emphasizing the importance of early intervention to prevent malnutrition and promote growth. Emerging research revealed that promising nutrients like resveratrol, along with modulation of the gut and respiratory microbiomes, may offer therapeutic advances for managing pulmonary hypertension. However, the current literature is limited by a lack of pediatric-specific studies, with much of the data extrapolated from adult populations and animal models, especially rats. This review highlights the need for targeted research to develop effective nutritional interventions aimed at improving outcomes for pediatric patients.
Dietary Approaches for Complementary Feeding: The Greek Mediterranean Diet as a Model for Caregivers
Authors: Eleftheriou Sofia , Vassilopoulou Emilia , Barbouni Anastasia , Chourdakis Michael , Kanellou Anastasia
Published: October 2024

Background/Objectives: Complementary feeding (CF), is defined as the process of providing foods in addition to milk when breast milk or milk formula alone are no longer adequate to meet nutritional requirements. CF affects not only growth but also the subsequent development of the child’s dietary preferences. The Mediterranean diet (MedDiet) is one of the most studied dietary patterns worldwide. The main purpose of this article is to provide practical guidance for CF to caregivers introducing the Greek MedDiet, summarizing the current different approaches. The evidence-based information provided could be used by developers to create a reliable digital app for CF based on Mediterranean foods and traditional dishes. Methods: An in-depth literature review of the existing guidelines and recently published research data on CF using PubMed, national recommendations, and grey literature were undertaken. Results: Practical, simple, evidence-based, comprehensive tables are presented. The tables serve as a guide to simplify the process of introducing Mediterranean foods in CF. Τhe tables consist of a total of more than 90 foods and day-to-day guidance for the first 13 weeks of CF. Furthermore, specific dietary guidance and suggestions regarding the order and content of meals during CF are given. Discussion: The tables are based on updated recommendations, summarizing the optimal feeding practices in a MedDiet-based perspective. Conclusions: Conclusively, there are significant contradictions among different organizations that present practical issues and cause confusion among caregivers. An effort was carried out to provide practical evidence-based guidance for caregivers introducing MedDiet during CF.
Enhancement of Family-Centred Care Is Associated with a Reduction in Postmenstrual Age at Discharge in Preterm Infants
Authors: Schuler Rahel , Eiben Carola , Waitz Markus , Neubauer Bernd A. , Hahn Andreas , Mihatsch Walter A.
Published: October 2024

Background/Objectives: Long hospitalisation has been recognized as an independent risk factor for poor neurodevelopmental outcomes of preterm infants. Systematic training and early inclusion of parents in their preterm infant’s care is a strategy to shorten the length of hospital stay. We implemented an enhanced stepwise family-centred care program and assessed its effects on postmenstrual age (PMA) at discharge and parental satisfaction. Methods: This prospective single-centre longitudinal cohort study was carried out in a German level III neonatal unit from October 2020 to May 2023. Five consecutive 6-month cohorts (1 baseline and 4 intervention cohorts, 169 infants and their caregivers) were analysed. Results: Mean PMA at discharge did not change in the total cohort but declined significantly in patients without neonatal morbidities from baseline to cohort 4 (37.2 ± 1.4 to 36.1 ± 1.6 weeks; p = 0.036). Concomitantly, discharge with tube feeding raised from 2.4% to 74.1% (p < 0.001) and discharge with home monitoring raised from 9.8% to 74.1% (p < 0.001), while unplanned readmissions remained unchanged (p = 0.44). Parental satisfaction with time point of discharge increased non-significantly from baseline to cohort 4 (75.8% vs. 95.7%; Chi2 0.22). Conclusions: Discharge of preterm infants at a significantly lower PMA is feasible through enhancement of family-centred care and is very well accepted by parents.
Superhydrophobic Coating Based on Nano-Silica Modification for Antifog Application of Partition Glass
Authors: Yu Linfei , Ma Kaiyang , Yin Hong , Zhou Chenliang , He Wenxiu , Yu Gewen , Zhang Qiang , Liu Quansheng , Zhao Yanxiong
Published: October 2024

In this study, vinyl triethoxysilane (VTES), KH-560 and trimethylchlorosilane (TMCS) were used to modify the surface groups of commercially available nano-silica (SiO2, 50 nm), and ethylene vinyl acetate copolymer (EVA) was used as a film-forming agent. EVA/SiO2, EVA/V-SiO2, EVA/K-SiO2 and EVA/T-SiO2 coatings were prepared, respectively. The coatings were characterized by SEM, FTIR, TG and contact angle. It was found that when the mass percentage of SiO2 was 66 wt%, the hydrophobicity performance of the coating could be significantly improved by silica modification. Compared to the EVA/SiO2, the water contact angle (WCA) of the EVA/V-SiO2, EVA/K-SiO2 and EVA/T-SiO2 were increased by 24.0%, 14.4% and 24.6%, respectively. The FTIR results indicated that VTES, KH-560 and TMCS could effectively replace the -OH groups on the surface of the SiO2 after hydrolysis, resulting in the presence of water transport groups on the SiO2 surface. The TG results certified that TMCS had the highest substitution rate (24.6%) for the -OH groups on the SiO2 surface after the hydrolysis. Additionally, the SEM results indicated that T-SiO2 was more easily dispersed in the EVA film-forming agent, leading to a uniform micro–nano surface rough structure, which aligned with the Cassie–Wenzel model. The durability test had demonstrated that the EVA/T-SiO2 maintained its hydrophobic properties even after enduring 40,000 drops of water and the impact of 200 g of sand. Furthermore, it exhibited excellent resistance to acid corrosion, along with superior self-cleaning properties and an anti-fog performance. It also provided outstanding protection against high temperatures and UV radiation for outdoor applications.
Navigating Digital Transformation in the UAE: Benefits, Challenges, and Future Directions in the Public Sector
Authors: Alzarooni Abdelrahim I. , Alhashmi Saadat M. , Lataifeh Mohammed , Rice John
Published: October 2024

Digital transformation is a process in which the latest technologies are used in various business fields to keep pace with continuous changes. It involves the strategic and profound integration of digital technologies into an organization’s core business operations, processes, and models. In this study, a quantitative approach was used to study the impact of DT adoption on public sector transformational change projects in the United Arab Emirates (UAE). The diffusion of innovation theory (DIT) and the unified theory of acceptance and use of technology model (UTAUT) were used in the factor analysis. This study highlights that digital transformation initiatives in the UAE have benefited from a strategic alignment with government initiatives, such as AI and blockchain strategies. However, public sector organizations face challenges, such as the high costs of technology adoption and cybersecurity risks during integration with legacy systems. The significance of social influence, including elements like use behavior and behavioral intention, was identified as essential for digital transformation, suggesting the importance of technology in job performance. Similarly, digital transformation projects improve IT competence and reduce resistance to change among leaders and individuals. The findings underscore the importance of investing in infrastructure and continuous IT training to sustain digital transformation. More studies are required across specific sectors to further explore the impact and scalability of DT initiatives in the UAE public sector.
A Taxonomic Revision of the South African Genus Plastologus Schoenherr, 1842 (Coleoptera, Curculionidae, Cyclominae)
Authors: Košťál Michael
Published: October 2024

Plastologus Schoenherr, 1842, hitherto a monotypic genus belonging to the tribe Hipporhinini, is herein revised for the first time. Three species are recognized as valid based on morphological characters, all distributed in southern regions of South Africa. In addition to the type species of the genus, Plastologus costatus Boheman, 1842, the following two species are described as new to the science: P. boroveci Košťál sp. n. and P. punctatus Košťál sp. n.
Combined Oxygen–Ozone and Porcine Injectable Collagen Therapies Boosting Efficacy in Low Back Pain and Disability
Authors: De Pascalis Manuela , Mulas Susanna , Sgarbi Liliana
Published: October 2024

Background/Objectives: Intervertebral disc degeneration is the most common cause of low back pain (LBP), and lumbosciatica is a major challenge to healthcare systems worldwide. For years, ozone therapy has been used with excellent results in intervertebral disc disease and in patients with LBP. In vitro studies have demonstrated the positive action of porcine collagen in extracellular matrix remodeling and homeostasis. These tissue changes, associated with LBP, may suggest an indication for combined ozone/collagen treatment in patients with LBP. However, no studies have been reported regarding this combination of treatments. Methods: The present work compared retrospective data of two treatment groups (each of 10 LBP patients): (A) oxygen–ozone therapy (OOT) vs. (B) OOT plus porcine collagen type 1 injections (COL I). Pain intensity and physiological function were assessed by the numerical rating scale (NSR) method. The Roland–Morris questionnaire was used to assess disability. Patient data were acquired before, during, and at the six-month follow-up. Significant differences were assessed by ANOVA and Student’s t-test. Results: The analyses revealed significant statistical differences comparing the two arms, where the (OOT+COL I) treatment demonstrated a booster efficacy in pain (a reduction of 62% vs. 35%), while the questionnaire revealed a reduction in disability (70% vs. 31%). Conclusions: Therefore, this combination therapy (oxygen–ozone plus porcine injectable collagen) might be a promising approach for the management of patients with LBP.
Polymorphism rs564398 of the ANRIL Gene as a Coronary-Artery-Disease-Associated SNP in Diabetic Patients of the Kazakh Population
Authors: Aitkaliyev Alisher , Bekenova Nazira , Vochshenkova Tamara , Kassiyeva Balzhan , Benberin Valeriy
Published: October 2024

Background/Objectives. A cardiovascular complication of type 2 diabetes mellitus like coronary artery disease is influenced by a complex interplay between environmental, phenotypic, and genetic factors. The genetic mechanisms in the development of this pathology are not established. This study aims to evaluate the association of polymorphisms rs1011970, rs62560775, and rs564398 from the 9p21.3 locus with coronary artery disease in diabetic patients of the Kazakh population. Methods. A total of 343 people participated in the case-control study: the control group consisted of 109 people with type 2 diabetes and coronary artery disease, while the control group included 234 people. Genotyping was performed using real-time PCR. Statistical analysis was carried out using Chi-square methods and calculating odds ratios (OR) with 95% confidence intervals (CI). Results. According to the results, only the rs564398 polymorphism of the ANRIL gene was associated with coronary artery disease (p = 0.04). The CC genotype increased the risk of developing coronary artery disease by more than 1.5 times (1.62 (1.02–2.56)), whereas the TT genotype reduced the risk of coronary artery disease (0.39 (0.17–0.91)). The remaining polymorphisms, rs1011970 and rs62560775, were not associated with coronary artery disease. Conclusions. Thus, this research further elicits the association of the ANRIL gene with cardiometabolic disease.
Comprehensive Insight into Regular Damped Oscillatory Structures from Effective Electromagnetic Form Factor Data of Some Mesons and Nucleons
Authors: Bartoš Erik , Dubnička Stanislav , Dubničková Anna Zuzana , Holka Lukáš , Liptaj Andrej
Published: October 2024

Regular damped oscillatory structures from the “effective” electromagnetic form factors of the hadrons h=π±,K±,K0,p,n were investigated. The “effective” electromagnetic form factor behaviors were calculated from the experimental data on the total cross-sections σtot(e+e−→hh¯) with errors. The apparent oscillations were observed for the first time for the proton, and we show, also taking other hadrons into consideration, that they are an arbitrary artifact resulting from a very simplistic theoretical description based on an elementary three-parameter model. If the data are described by a more appropriate and physically well-founded Unitary and Analytic model, then the oscillations disappear. In spite of this, if the three-parameter model is used to describe the “effective” electromagnetic form factor data, an interesting phenomenon is observed. The oscillations are opposite for particles which form an isospin doublet. By using the physically well-founded Unitary and Analytic model, it is demonstrated that this feature originates from the special transformation properties of the electromagnetic current of the corresponding particles in the isotopic space.
Partition Function Zeros of the Frustrated J1–J2 Ising Model on the Honeycomb Lattice
Authors: Gessert Denis , Weigel Martin , Janke Wolfhard
Published: October 2024

We study the zeros of the partition function in the complex temperature plane (Fisher zeros) and in the complex external field plane (Lee–Yang zeros) of a frustrated Ising model with competing nearest-neighbor (J1>0) and next-nearest-neighbor (J2<0) interactions on the honeycomb lattice. We consider the finite-size scaling (FSS) of the leading Fisher and Lee–Yang zeros as determined from a cumulant method and compare it to a traditional scaling analysis based on the logarithmic derivative of the magnetization ∂ln⟨|M|⟩/∂β and the magnetic susceptibility χ. While for this model both FSS approaches are subject to strong corrections to scaling induced by the frustration, their behavior is rather different, in particular as the ratio R=J2/J1 is varied. As a consequence, an analysis of the scaling of partition function zeros turns out to be a useful complement to a more traditional FSS analysis. For the cumulant method, we also study the convergence as a function of cumulant order, providing suggestions for practical implementations. The scaling of the zeros convincingly shows that the system remains in the Ising universality class for R as low as −0.22, where results from traditional FSS using the same simulation data are less conclusive. Hence, the approach provides a valuable additional tool for mapping out the phase diagram of models afflicted by strong corrections to scaling.
The Use of the Data Envelopment Analysis–Malmquist Approach to Measure the Performance of Digital Transformation in EU Countries
Authors: Horváthová Jarmila , Mokrišová Martina
Published: October 2024

Currently, the process of the digital transformation of EU countries is very important and often discussed. It will not only bring new opportunities for companies and the broader population but will also enable the transition to a more ecological economy. An important goal is to speed up the digitalization processes taking place in companies. It is very important to use already established digitalization elements more efficiently. This also resulted in the motivation for the given research. The aim of this paper is to quantify the change in the efficiency of the digital transformation of EU countries. As part of this research, the Variable Returns to Scale Data Envelopment Analysis (VRS DEA) model and the Malmquist index (MI) based on the DEA approach were applied. The results of the model made it possible to assess how the changes in technical efficiency and technological changes contributed to the changes in efficiency. The long-term theoretical added value of this paper lies in its proposal for countries and their governments to monitor not only the number of introduced digital elements, but also the efficiency of their use relative to some aggregate output; for example, GDP (Gross Domestic Product) or unemployment rate. The added value of this research is that less developed countries use digitalization elements more effectively than developed countries.