bluuebunny/crossref_metadata_2025 huggingface dataset:
Place-and-Route Analysis of FPGA Implementation of Nested Hardware Self-Organizing Map Architecture
Authors: Hikawa Hiroomi
Published: November 2023

Self-organizing map (SOM) is a type of artificial neural network that provides a nonlinear mapping from a given high-dimensional input space to a low-dimensional map of neurons for clustering. The clustering of high-dimensional vectors is too slow for SOM when implemented in software. In such cases, an application-specific hardware SOM accelerator is highly desirable. Field-programmable gate array (FPGA) implementation is a popular platform to implement hardware SOM. In our previous work, a nested hardware SOM architecture, which has a homogeneous modular structure to enhance expandability, was proposed. This paper investigates the impact of the nested hardware SOM on FPGA implementation tools that perform logic synthesis, place, and route (PAR). Experiments revealed that the nested architecture provided better results in resource usage and performance. FPGA resource usage of the nested architecture was 97.2% of that of the flat design on average. Importantly, the nested architecture operated at 10% higher clock frequencies compared to flat SOM designs. In addition, the pipeline computation was improved by increasing the pipeline stages so that it operates with a higher clock frequency. The operable clock frequency was 81 MHz, which was 21 MHz higher than its predecessor.
Addressing Challenges and Outcomes in the Biogas Sector: An Analysis of Efficiency, Economic Savings, and Environmental Impacts Using an Advanced SWOT Model
Authors: Esposito Luca , Vecchio Chiara , Cattaneo Giancarlo , Gu Zhouyi , Scotto di Perta Ester
Published: November 2023

This study, through the use of company data, offers an in-depth analysis of the current situation and future prospects of biogas plants and how they can promote economic advantages and environmental benefits. The geographical context of the Piana del Sele (Salerno, Italy) was chosen on the basis of objective criteria, including its relevance in the agro-industrial sector and the availability of accurate and reliable company data that was collected through IT systems, including software business performance monitoring and digital data analysis. This choice was fundamental to guaranteeing the validity and representativeness of our analyses and results. From a methodological point of view, mathematical formulas and algorithms were used, which allowed the calculation of the biogas producibility indices by type of biomass as well as the quantification of the effluents produced to then estimate the quantification of the biogas and the interconnected economic savings. Furthermore, this study uses the SWOT (strengths, weaknesses, opportunities, and threats) approach in an innovative way by integrating renewable energy communities into it. The aim is to demonstrate the potential of biogas plants and how energy communities can transform weaknesses and threats into opportunities. The results show that the integration of biogas plants into the farm environment offers considerable potential, and although it is a challenge for small and medium-sized farms, it could bring energy self-sufficiency and economic surplus. Furthermore, the integration of renewable energy communities would be able to promote the diversification of energy supply and transform weaknesses and threats into opportunities.
Dielectric Breakdown Strength of PDMS Elastomers after Mechanical Cycling
Authors: Taine Emmanuel , Andritsch Thomas , Saeedi Istebreq A. , Morshuis Peter H. F.
Published: November 2023

PDMS-based composites such as silicone elastomers are commonly found in high-voltage engineering, especially in outdoor insulation as coatings or structural elements or at interfaces between network elements, such as cable sealing ends (CSE). They are also promising prospects for dielectric elastomer generators (DEG), which are retrieving electrostatic energy from large strain amplitudes. The upper limit of energy conversion from these transducers is determined by the dielectric breakdown strength (DBS). Therefore, developing reliable systems that operate under high electric fields and variable repeated strains requires a thorough understanding of the mechanisms behind electrical breakdown and its coupling to mechanical cycling. In this study, the effect of Mullins damage and mechanical fatigue on silicone elastomers has been investigated. An electro-mechanical instability model that considers cyclic softening allows for predicting the evolution of the breakdown strength depending on the loading history. The results highlight the importance of the “first cycle,” where up to a 30% reduction in the mean DBS was measured. However, subsequent mechanical fatigue only marginally contributes to the degradation, which is a promising perspective for the long-term performance of any silicone elastomer as long as the precise impact of the first cycle is known.
Biogas Production—The Effect of the Zinc Concentration on the Profile of Volatile Fatty Acids in Fermentation Mixtures
Authors: Cichosz Marcin , Łazarski Sławomir , Butarewicz Andrzej , Kiełkowska Urszula
Published: November 2023

The development of renewable energy sources is one of the most important paths for today’s economy to follow. Currently, the process of obtaining energy from the combustion of methane extracted by methane fermentation is gaining importance. To improve its efficiency and the speed of the fermentation process and enzyme activation, this study analyzed the effect of the addition of zinc as a micronutrient to fermentation processes using corn silage. The zinc concentration in the fermenter required to stimulate fermentation processes was determined. Studies were conducted to determine inhibitory and toxic concentrations. The determination of the influence of zinc ions on the VFA profile and the value of the carbon-to-methane conversion coefficient is important for the economic aspects of obtaining biogas with the highest methane content. The carbon-to-methane conversion factor and the effect of the addition of zinc on the value of this factor were determined. During the course of the research, modern analytical chemistry methods and techniques were used. The purpose of this study was to determine the optimal concentration of zinc in the digester to maximize the methane yield of the fermentation process.
Partial Discharge Localization through k-NN and SVM
Authors: Sekatane Permit Mathuhu , Bokoro Pitshou
Published: November 2023

Power transformers are essential for the distribution and transmission of electricity, but they are prone to degradation due to faults early on. Partial Discharge (PD) is the most significant pointer of insulation breakdown in high-voltage apparatus. Dissolved Gas Analysis (DGA) is a commonly used technique for detecting and diagnosing PD. However, DGA data often contain missing values, which can significantly affect the accuracy of PD diagnosis. To mitigate the issues of missing values, this paper proposes using the k-Nearest Neighbors (kNN) technique to impute the missing values in the dataset. Further, it combines kNN with a Support Vector Machine (SVM) to detect the possibility of a PD source in the high-voltage apparatus. The approach was evaluated on a real-world DGA dataset and achieved high classification performance and discriminatory power for distinguishing between PD and non-PD instances. The effectiveness of the missing value imputation technique was evaluated, and the proposed approach demonstrated improved accuracy and precision compared to methods without imputation. The proposed approach offers a current solution for PD analysis in power transformers using DGA data with missing values.
What Is the Impact of the Renewable Energy Power Absorption Guarantee Mechanism on China’s Green Electricity Market?
Authors: Lu Yan , Liu Xuan , Li Hongjian , Wang Haoran , Kong Jiajie , Zhong Cheng , Cui Mingli , Li Yan , Sun Xiaoqi , Xuan Jiadong , Feng Tiantian
Published: November 2023

In order to accelerate the construction of a clean, low-carbon, safe and efficient energy system, China set the provincial weight of responsibility for renewable energy power consumption and established a renewable energy power absorption guarantee mechanism in 2019. As a market incentive policy, it has enduring effect on the low-carbon transformation of the power industry. Firstly, the operation mechanism of the renewable energy consumption guarantee mechanism is analyzed. The general framework, core elements and supporting measures are clarified. Secondly, a stock-flow diagram is constructed based on the system dynamics method. It contains the green electricity market sub-module, the green-certificate market sub-module and the excess power absorption market sub-module. Finally, multiple scenarios are set up to simulate the impact of the green-certificate market and excess power absorption market improvements on the installed capacity and tariff of China’s green power market. The renewable energy guarantee mechanism is an effective means to promote the consumption of green electricity in China. In addition, in the short term the cost of electricity for users has increased, but in the long term the cost of electricity shows a fluctuating downward trend. This study provides theoretical references for the formulation of clean and low-carbon policy objectives in the power industry and the optimization of market mechanisms.
Impacts and Predictions of Urban Expansion on Habitat Connectivity Networks: A Multi-Scenario Simulation Approach
Authors: Chang Shihui , Su Kai , Jiang Xuebing , You Yongfa , Li Chuang , Wang Luying
Published: November 2023

Urban expansion is leading to the loss and fragmentation of habitats, which poses a threat to wildlife. People are hopeful that, through scientific urban planning and the adoption of innovative models for human communities, such a situation can be improved. Thus, a case study was carried out in Nanning City, China, to extract habitats, build an ecological resistance surface, and construct a habitat connectivity network (HCN). To simulate changes to unused land in the future, we put forth the A (the parcel is divided into strips), B (the parcel is divided into two strips), C (the central area of the parcel is planned as a quadrangle), and D (opposite to Scenario C, the peripheral area is green space) scenarios of human communities that guarantee a 30% ratio of green space, and established the corresponding HCNs. The results indicate that: (1) Currently, the habitats cover approximately 153.24 km2 (34.08%) of the entire study area. The ecological corridors in this region amount to a total of 5337, and the topological indicators and robustness indicate a strong stability of the current HCN. (2) With urban expansion, once continuous habitats are being fragmented into smaller green spaces, it is estimated that the habitats will shrink by 64.60 km2. The topological indicators and robustness reveal that the stability of the HCNs becomes lower as well. Multiple scenario simulations demonstrated that Scenario D is better than Scenarios B and C, while Scenario A performed the worst. (3) Furthermore, we observed a stronger negative impact of urban expansion on local connectivity. This indicates that the influence of urban expansion on the local HCNs is often more pronounced and may even be destructive. Our findings can advise urban planners on decisions to minimize the impact of urban expansion on wildlife.
Increased Vegetation Productivity of Altitudinal Vegetation Belts in the Chinese Tianshan Mountains despite Warming and Drying since the Early 21st Century
Authors: Zhang Yong , An Chengbang , Jiang Lai , Zheng Liyuan , Tan Bo , Lu Chao , Zhang Wensheng , Zhang Yanzhen
Published: November 2023

Gaining a deep understanding of how climate change affects the carbon cycle in dryland vegetation is of utmost importance, as it plays a pivotal role in shaping the overall carbon cycle in global ecosystems. It is currently not clear how plant communities at varying elevations in arid mountainous regions will respond to climate change in terms of their productivity. The aim of this study was to investigate the effect of climate change on vegetation productivity in different altitudinal vegetation belts of the Tianshan Mountains between 2000 and 2021, utilizing satellite-derived vegetation productivity and climate data. The findings suggest a notable increase in vegetation productivity across diverse altitudinal vegetation belts. The productivity of vegetation in the coniferous forest and alpine meadow belts displayed a notably higher interannual trend compared to other vegetation belts. Notably, an increase in vegetation productivity was accompanied by warming and drying. The productivity of altitudinal vegetation belts, however, appears to be resilient to current climate trends and was not significantly impacted by the severity of atmospheric drought. The trend of increased vegetation productivity was primarily driven by CO2 fertilization. Our results highlight that the extent of climate change may need to reach a threshold to noticeably affect variations in vegetation productivity in arid mountainous.
Social Value of Urban Green Space Based on Visitors’ Perceptions: The Case of the Summer Palace, Beijing, China
Authors: Huang Ruoyi , Liu Yunxi , Liang Shu , Si Jiayi , Di Shuyi , Cai Mengmeng , Hu Shuang , Hao Chunxu , Zhao Zheng
Published: November 2023

Urban green spaces play a key role in constructing an ecological civilization in China. In this context, the realization and assessment of the social value of urban green spaces have received increasing attention. Taking the visitors of the Summer Palace in Beijing as the research object, this study used the public participation geographic information system (PPGIS) to collect research data, assess the social value of ecosystem services in the Summer Palace, and determine its spatial distribution. By examining the social value of urban green spaces, this study explored the relationship between the spatial distribution of ecosystem social value and environmental landscape features. The influence of different environmental values on the spatial distribution of the visitors’ perceived social value was also investigated to provide a decision-making basis for the planning and management of urban ecological spaces and the supply and protection of urban ecological products. This study found that cultural, historical, and aesthetic values were preferred by an increasing number of visitors. The hotspots of social value in the Summer Palace of Beijing have three cores and multiple centers. By analyzing the relationship between the three value types with higher preference and the environmental landscape features of the Summer Palace, this study found that the distribution of social values is closely related to the architectural complexes and waters. Anthropocentrists have smaller sample sizes and generally lower social value indices within the sample group, and ecocentrists have higher perceptions of overall social values.
Application of Thermo-Malaxation Followed by Three-Phase Centrifugation to Enable the Biorefinery of Alperujo, the Main By-Product of Olive Oil
Authors: Fernández-Prior África , Bermúdez-Oria Alejandra , Rubio-Senent Fátima , Villanueva-Lazo Álvaro , Fernández-Bolaños Juan , Rodríguez-Gutiérrez Guillermo
Published: November 2023

The pomace olive oil sector needs to improve its use of the main olive oil by-product, called alperujo, which is currently used mainly for combustion after extraction of pomace oil, with all the problems this process entails due to the high degree of humidity, organic load and phytotoxic substances. In this work, a solution at an industrial level that uses thermo-malaxation at a temperature close to 65 °C for one or two hours followed by centrifugation in three phases is proposed. In this way, over 40% of the pomace oil that is rich in minor compounds, a solid with a lower degree of humidity (55%), and a liquid aqueous fraction that is rich in bioactive compounds such as phenolics and sugars are obtained. This aqueous fraction can be treated through subsequent storage stages to increase its content of the main phenolic, hydroxytyrosol, to up to 1.77 g/L, decreasing its percentage of insoluble solids by up to 1.9%, making it possible to obtain extracts that are rich in hydroxytyrosol using systems that are commonly in place at the industrial level. The aqueous fraction, without phenolics, could be used for energy production. A solid with a slightly higher fat content than the initial alperujo remains, thus the rest of the oil content can be extracted from it using solvent, making it, once defatted, suitable for application in subsequent bioprocesses.
New Lizard Specimens from the Campanian Wapiti Formation of Alberta, Canada
Authors: Hamilton Samantha M. , Paparella Ilaria , Bell Phil R. , Campione Nicolás E. , Fanti Federico , Larson Derek W. , Sissons Robin L. , Vavrek Matthew J. , Balsai Michael J. , Sullivan Corwin
Published: November 2023

Reported lizard material from the Wapiti Formation (central-western Alberta, Canada) is limited to fragmentary remains of Kleskunsaurus grandeprairiensis and Socognathus unicuspis, a partial dentary attributed to Chamops cf. C. segnis, and a vertebra reportedly comparable to those of the much larger lizard Palaeosaniwa canadensis. P. canadensis is a Late Cretaceous North American member of Monstersauria, a Mesozoic and Cenozoic anguimorph group represented today by five species of Heloderma. Here, we document new squamate material from the DC Bonebed locality (Wapiti Unit 3; Campanian), including a right frontal identified as cf. P. canadensis and a taxonomically indeterminate squamate astragalocalcaneum. A partial skeleton from the Two Medicine Formation of Montana provisionally attributed to P. canadensis has a frontal resembling the corresponding element from the DC Bonebed in overall shape, in having narrowly separated facets for the prefrontal and postorbitofrontal, and in bearing osteoderms similar to the DC specimen’s in ornamentation and configuration. The Two Medicine and DC specimens differ from a roughly contemporaneous frontal from southern Alberta referred to the monstersaur Labrodioctes montanensis. The DC specimen confirms the presence of monstersaurian squamates in the Wapiti Formation, representing the northernmost record of any definitive Late Cretaceous monstersaur to date.
Psychological Symptoms in Parents Who Experience Child-to-Parent Violence: The Role of Self-Efficacy Beliefs
Authors: Jiménez-Granado Aitor , Fernández-González Liria , del Hoyo-Bilbao Joana , Calvete Esther
Published: November 2023

Previous research suggests that parents involved in child-to-parent violence (CPV) experience shame, judgment, and a lack of social support, often accompanied by feelings of self-blame and helplessness as well as a deterioration in their perception of self-efficacy and their parenting skills. All of these factors may impact parents’ mental health. However, there is a research gap concerning the consequences of CPV among parents. Therefore, the objective of this study was to examine the relationship between CPV and psychological symptoms (i.e., depression, anxiety, hostility, obsessive–compulsive, interpersonal sensitivity, and somatization) in parents based on their perceptions of self-efficacy. The sample was composed of 354 participants: 177 parents (83.1% mothers) and their 177 children (53.4% boys; Mage = 13.27). CPV was reported by both parents and their children. In addition, parents reported their self-efficacy beliefs and psychological symptoms. The results showed that CPV was negatively associated with parents’ psychological symptomatology, except for somatization. Moreover, self-efficacy beliefs explain part of the indirect association between CPV behaviors and psychological symptoms in parents. Overall, our findings provide evidence for the potential impact of CPV on mental health in parents and suggest the relevance of reinforcing their self-efficacy beliefs.
Being a Young Carer in Portugal: The Impact of Caring on Adolescents’ Life Satisfaction
Authors: Meireles Ana , Marques Sofia , Faria Sara , Lopes Joana Correia , Teixeira Ana Ribas , Alves Bruno , Becker Saul
Published: November 2023

Caring for an ill or disabled relative can present significant challenges that may exceed the personal resources of the caregiver. Young carers (YCs) often take on this role, providing support to family members or friends, which can have far-reaching effects on various aspects of their lives. This study involved 235 adolescents, 106 YCs, and 129 non-carers (NCs), who completed questionnaires assessing life satisfaction, satisfaction with social support, family functioning, academic functioning, and caregiving activities. Tests of group differences (MANOVA and MANCOVA controlling for age) showed YCs had more caregiving activities than NCs (as expected) and, critically, significantly lower life satisfaction. Hierarchical regressions with the YCS subsample showed academic functioning, social support, and the negative impact of caregiving were associated with life satisfaction, and that the negative influence of caregiving was linked to family functioning and the quantity of caregiving activities. For NCs, academic functioning, satisfaction with social support, and family functioning were associated with life satisfaction. In conclusion, caregiving in adolescents appears to be linked to lower life satisfaction, but this effect is determined by their social support, academic functioning, and negative impact of caring, which in turn depends on their family functioning and amount of caring activities.
Transcriptome Profiling of Cardiac Glycoside Treatment Reveals EGR1 and Downstream Proteins of MAPK/ERK Signaling Pathway in Human Breast Cancer Cells
Authors: Pavithran Honey , Kumavath Ranjith , Ghosh Preetam
Published: November 2023

Cardiac glycosides (CGs) constitute a group of steroid-like compounds renowned for their effectiveness in treating cardiovascular ailments. In recent times, there has been growing recognition of their potential use as drug leads in cancer treatment. In our prior research, we identified three highly promising CG compounds, namely lanatoside C (LC), peruvoside (PS), and strophanthidin (STR), which exhibited significant antitumor effects in lung, liver, and breast cancer cell lines. In this study, we investigated the therapeutic response of these CGs, with a particular focus on the MCF-7 breast cancer cell line. We conducted transcriptomic profiling and further validated the gene and protein expression changes induced by treatment through qRT-PCR, immunoblotting, and immunocytochemical analysis. Additionally, we demonstrated the interactions between the ligands and target proteins using the molecular docking approach. The transcriptome analysis revealed a cluster of genes with potential therapeutic targets involved in cytotoxicity, immunomodulation, and tumor-suppressor pathways. Subsequently, we focused on cross-validating the ten most significantly expressed genes, EGR1, MAPK1, p53, CCNK, CASP9, BCL2L1, CDK7, CDK2, CDK2AP1, and CDKN1A, through qRT-PCR, and their by confirming the consistent expression pattern with RNA-Seq data. Notably, among the most variable genes, we identified EGR1, the downstream effector of the MAPK signaling pathway, which performs the regulatory function in cell proliferation, tumor invasion, and immune regulation. Furthermore, we substantiated the influence of CG compounds on translational processes, resulting in an alteration in protein expression upon treatment. An additional analysis of ligand–protein interactions provided further evidence of the robust binding affinity between LC, PS, and STR and their respective protein targets. These findings underscore the intense anticancer activity of the investigated CGs, shedding light on potential target genes and elucidating the probable mechanism of action of CGs in breast cancer.
Natural Chalcones for the Management of Obesity Disease
Authors: Maisto Maria , Marzocchi Adua , Keivani Niloufar , Piccolo Vincenzo , Summa Vincenzo , Tenore Gian Carlo
Published: November 2023

In the last decade, the incidence of obesity has increased dramatically worldwide, reaching a dangerous pandemic spread. This condition has serious public health implications as it significantly increases the risk of chronic diseases such as type 2 diabetes, fatty liver, hypertension, heart attack, and stroke. The treatment of obesity is therefore the greatest health challenge of our time. Conventional therapeutic treatment of obesity is based on the use of various synthetic molecules belonging to the class of appetite suppressants, lipase inhibitors, hormones, metabolic regulators, and inhibitors of intestinal peptide receptors. The long-term use of these molecules is generally limited by various side effects and tolerance. For this reason, the search for natural alternatives to treat obesity is a current research goal. This review therefore examined the anti-obesity potential of natural chalcones based on available evidence from in vitro and animal studies. In particular, the results of the main in vitro studies describing the principal molecular therapeutic targets and the mechanism of action of the different chalcones investigated were described. In addition, the results of the most relevant animal studies were reported. Undoubtedly, future clinical studies are urgently needed to confirm and validate the potential of natural chalcones in the clinical prophylaxis of obesity.
The Repurposing of Non-Peptide Neurokinin-1 Receptor Antagonists as Antitumor Drugs: An Urgent Challenge for Aprepitant
Authors: Coveñas Rafael , Rodríguez Francisco D. , Robinson Prema , Muñoz Miguel
Published: November 2023

The substance P (SP)/neurokinin-1 receptor (NK-1R) system is involved in cancer progression. NK-1R, activated by SP, promotes tumor cell proliferation and migration, angiogenesis, the Warburg effect, and the prevention of apoptosis. Tumor cells overexpress NK-1R, which influences their viability. A typical specific anticancer strategy using NK-1R antagonists, irrespective of the tumor type, is possible because these antagonists block all the effects mentioned above mediated by SP on cancer cells. This review will update the information regarding using NK-1R antagonists, particularly Aprepitant, as an anticancer drug. Aprepitant shows a broad-spectrum anticancer effect against many tumor types. Aprepitant alone or in combination therapy with radiotherapy or chemotherapy could reduce the sequelae and increase the cure rate and quality of life of patients with cancer. Current data open the door to new cancer research aimed at antitumor therapeutic strategies using Aprepitant. To achieve this goal, reprofiling the antiemetic Aprepitant as an anticancer drug is urgently needed.
Meconium Transferrin and Ferritin as Markers of Homeostasis in the Developing Fetus
Authors: Skarżyńska Ewa , Mularczyk Klaudia , Issat Tadeusz , Jakimiuk Artur , Lisowska-Myjak Barbara
Published: November 2023

The molecular mechanisms regulating homeostasis in the developing fetus have not been satisfactorily elucidated. Meconium contains substances accumulated in the fetal intestines. Measurements of transferrin and ferritin concentrations in meconium and assessment of transferrin–ferritin relationships could enhance knowledge about specific processes of the intrauterine period involving the two proteins and their effects on the development and growth of the fetus. Transferrin and ferritin concentrations were measured by ELISA in the homogenates of first meconium portions from 125 neonates. Higher birth weight was associated with lower ferritin concentrations in meconium (r = −0.22, p = 0.015). In neonates with a birth weight of more than 3750 g, there was a positive correlation between transferrin and ferritin concentrations (r = 0.51, p = 0.003). With meconium transferrin concentrations above 43.52 µg/g, a negative correlation between transferrin and ferritin was established (r = −0.37, p = 0.036), while with transferrin concentrations below 43.52 µg/g, the correlations between the birth weight and the meconium transferrin and ferritin concentrations were negative (r = −0.61, p < 0.001 and r = −0.43, p = 0.017, respectively). Measurements of transferrin and ferritin in meconium specimens create a new use for these common biomarkers to improve our understanding of the effects of homeostasis in utero on the fetal development and growth. Establishing reference ranges of meconium transferrin and ferritin concentrations and their association with the clinical parameters during pregnancy could aid in the assessment of the impact of intrauterine life on the health status of the neonate and its adaptation to extrauterine life.
Genome-Wide Analysis of bHLH Family Genes and Identification of Members Associated with Cold/Drought-Induced Photoinhibition in Kandelia obovata
Authors: Li Junjian , Chen Siyi , Yin Yaxin , Shan Qiaobo , Zheng Chunfang , Chen Yan
Published: November 2023

Plant basic helix-loop-helix (bHLH) transcription factors play pivotal roles in responding to stress, including cold and drought. However, it remains unclear how bHLH family genes respond to these stresses in Kandelia obovata. In this study, we identified 75 bHLH members in K. obovata, classified into 11 subfamilies and unevenly distributed across its 18 chromosomes. Collineation analysis revealed that segmental duplication primarily drove the expansion of KobHLH genes. The KobHLH promoters were enriched with elements associated with light response. Through RNA-seq, we identified several cold/drought-associated KobHLH genes. This correlated with decreased net photosynthetic rates (Pn) in the leaves of cold/drought-treated plants. Weighted gene co-expression network analysis (WGCNA) confirmed that 11 KobHLH genes were closely linked to photoinhibition in photosystem II (PS II). Among them, four Phytochrome Interacting Factors (PIFs) involved in chlorophyll metabolism were significantly down-regulated. Subcellular localization showed that KobHLH52 and KobHLH30 were located in the nucleus. Overall, we have comprehensively analyzed the KobHLH family and identified several members associated with photoinhibition under cold or drought stress, which may be helpfulfor further cold/drought-tolerance enhancement and molecular breeding through genetic engineering in K. obovata.
Downregulation of the INDEHISCENT Gene by RNAi Resulted in Desired Pod Shatter Reduction of Lepidium campestre in Subsequent Generations
Authors: Ivarson Emelie , Ahlman Annelie , Englund Jan-Eric , Lager Ida , Zhu Li-Hua
Published: November 2023

Wild species field cress (Lepidium campestre) has favorable agronomic traits, making it a good candidate for future development as an oil and catch crop. However, the species is very prone to pod shatter, resulting in severe yield losses. This is one of the important agronomic traits that needs to be improved in order to make this species economically viable. In this study, we cloned the L. campestre INDEHISCENT (LcIND) gene and prepared two LcIND-RNAi constructs with the IND promoter (long 400 bp and short 200 bp) from Arabidopsis. A number of stable transgenic lines were developed and evaluated in terms of pod shatter resistance. The majority of the transgenic lines showed increased resistance to pod shatter compared to the wild type, and this resistance was maintained in four subsequent generations. The downregulation of the LcIND gene by RNAi in the transgenic lines was confirmed by qRT-PCR analysis on T3 lines. Southern blot analysis showed that most of the analyzed lines had a single-copy integration of the transgene, which is desirable for further use. Our results show that it is possible to generate stable transgenic lines with desirable pod shatter resistance by downregulating the LcIND gene using RNAi in field cress, and thus speeding up the domestication process of this wild species.
Targeting Progranulin as an Immuno-Neurology Therapeutic Approach
Authors: Boylan Maria A. , Pincetic Andrew , Romano Gary , Tatton Nadine , Kenkare-Mitra Sara , Rosenthal Arnon
Published: November 2023

Immuno-neurology is an emerging therapeutic strategy for dementia and neurodegeneration designed to address immune surveillance failure in the brain. Microglia, as central nervous system (CNS)-resident myeloid cells, routinely perform surveillance of the brain and support neuronal function. Loss-of-function (LOF) mutations causing decreased levels of progranulin (PGRN), an immune regulatory protein, lead to dysfunctional microglia and are associated with multiple neurodegenerative diseases, including frontotemporal dementia caused by the progranulin gene (GRN) mutation (FTD-GRN), Alzheimer’s disease (AD), Parkinson’s disease (PD), limbic-predominant age-related transactivation response deoxyribonucleic acid binding protein 43 (TDP-43) encephalopathy (LATE), and amyotrophic lateral sclerosis (ALS). Immuno-neurology targets immune checkpoint-like proteins, offering the potential to convert aging and dysfunctional microglia into disease-fighting cells that counteract multiple disease pathologies, clear misfolded proteins and debris, promote myelin and synapse repair, optimize neuronal function, support astrocytes and oligodendrocytes, and maintain brain vasculature. Several clinical trials are underway to elevate PGRN levels as one strategy to modulate the function of microglia and counteract neurodegenerative changes associated with various disease states. If successful, these and other immuno-neurology drugs have the potential to revolutionize the treatment of neurodegenerative disorders by harnessing the brain’s immune system and shifting it from an inflammatory/pathological state to an enhanced physiological/homeostatic state.
Effect of Hydralazine on Angiotensin II-Induced Abdominal Aortic Aneurysm in Apolipoprotein E-Deficient Mice
Authors: Wang Yutang , Sargisson Owen , Nguyen Dinh Tam , Parker Ketura , Pyke Stephan J. R. , Alramahi Ahmed , Thihlum Liam , Fang Yan , Wallace Morgan E. , Berzins Stuart P. , Oqueli Ernesto , Magliano Dianna J. , Golledge Jonathan
Published: November 2023

The rupture of an abdominal aortic aneurysm (AAA) causes about 200,000 deaths worldwide each year. However, there are currently no effective drug therapies to prevent AAA formation or, when present, to decrease progression and rupture, highlighting an urgent need for more research in this field. Increased vascular inflammation and enhanced apoptosis of vascular smooth muscle cells (VSMCs) are implicated in AAA formation. Here, we investigated whether hydralazine, which has anti-inflammatory and anti-apoptotic properties, inhibited AAA formation and pathological hallmarks. In cultured VSMCs, hydralazine (100 μM) inhibited the increase in inflammatory gene expression and apoptosis induced by acrolein and hydrogen peroxide, two oxidants that may play a role in AAA pathogenesis. The anti-apoptotic effect of hydralazine was associated with a decrease in caspase 8 gene expression. In a mouse model of AAA induced by subcutaneous angiotensin II infusion (1 µg/kg body weight/min) for 28 days in apolipoprotein E-deficient mice, hydralazine treatment (24 mg/kg/day) significantly decreased AAA incidence from 80% to 20% and suprarenal aortic diameter by 32% from 2.26 mm to 1.53 mm. Hydralazine treatment also significantly increased the survival rate from 60% to 100%. In conclusion, hydralazine inhibited AAA formation and rupture in a mouse model, which was associated with its anti-inflammatory and anti-apoptotic properties.
Guijing2501 (Citrus unshiu) Has Stronger Cold Tolerance Due to Higher Photoprotective Capacity as Revealed by Comparative Transcriptomic and Physiological Analysis and Overexpression of Early Light-Induced Protein
Authors: Xiao Cui , He Ligang , Qiu Wenming , Wang Zeqiong , He Xiujuan , Xiao Yuxiong , Sun Zhonghai , Tong Zhu , Jiang Yingchun
Published: November 2023

Cold is one of the major limiting factors for citrus production, particularly extreme cold waves. Therefore, it is of great importance to develop cold-tolerant varieties and clarify their cold tolerance mechanisms in citrus breeding. In this study, comparative transcriptomic and physiological analyses were performed to dissect the cold tolerance mechanism of Guijing2501 (GJ2501), a new satsuma mandarin (Citrus unshiu) variety with about 1 °C lower LT50 (the median lethal temperature) relative to Guijing (GJ). The physiological analysis results revealed that GJ2501 is more cold-tolerant with less photoinhibition, PSII photodamage, and MDA accumulation, but higher POD activity than GJ under cold stress. Comparative transcriptomic analysis identified 4200 DEGs between GJ and GJ2501, as well as 4884 and 5580 up-regulated DEGs, and 5288 and 5862 down-regulated DEGs in response to cold stress in GJ and GJ2501, respectively. “Photosynthesis, light harvesting” and “photosystem” were the specific and most significantly enriched GO terms in GJ2501 in response to cold stress. Two CuELIP1 genes (encoding early light-induced proteins) related to the elimination of PSII photodamage and photoinhibition were remarkably up-regulated (by about 1000-fold) by cold stress in GJ2501 as indicated by RT-qPCR verification. Overexpression of CuELIP1 from GJ2501 in transgenic Arabidopsis protected PSII against photoinhibition under cold stress. Taken together, the cold tolerance of GJ2501 may be ascribed to its higher photoprotective capacity under cold stress.
The Left Atrial Appendage and Atrial Fibrillation—A Contemporary Review
Authors: Sulague Ralf Martz , Whitham Tarik , Danganan Lester Mico Lopez , Effiom Victory , Candelario Katherine , Latif Nida , Hameed Irbaz
Published: November 2023

In patients with atrial fibrillation, the left atrial appendage may serve as the site of thrombus formation due to stasis that occurs within the appendage because of its shape and trabeculations. Although thrombus formation can be reduced by using anticoagulants, this may be contraindicated in some patients. The need for a better alternative treatment prompted the study of left atrial appendage occlusion for thromboembolism prophylaxis. Due to this, procedures that excise or occlude the left atrial appendage have gained attention because of their ability to prevent thromboembolic events. This article provides a comprehensive review of the left atrial appendage and its associated procedures’ clinical utility.
Impact of Vein of Marshall Ethanol Infusion Combined with Anatomical Ablation for the Treatment of Persistent Atrial Fibrillation: A Long-Term Follow-Up Based on Implantable Loop Recorders
Authors: Nesti Martina , Luca Fabiana , Panchetti Luca , Garibaldi Silvia , Startari Umberto , Mirizzi Gianluca , Landra Federico , Giannoni Alberto , Piacenti Marcello , Rossi Andrea
Published: November 2023

Background: The best ablation treatment for persistent atrial fibrillation (PeAF) patients is still debated. The vein of Marshall (VOM) seems to be a promising target for ablation and could be combined with a linear set of ablation lesions. The aim of our study is to evaluate the incidence of AF recurrences in a PeAF population treated with a comprehensive ablation approach consisting of VOM ethanol infusion (EI), pulmonary vein isolation (PVI), a left atrial (LA) roofline, a mitral line (guided by the newly formed lesion after alcohol infusion into the VOM and validated by pacing), and a cavotricuspid isthmus line. Methods: Consecutive patients undergoing the first ablation procedure of catheter ablation (CA) for PeAF were enrolled. All patients underwent VOM-EI, PVI, and ablation lines along the roof of the LA, mitral, and cavotricuspid isthmus. LA voltage mapping before and after VOM-EI was also performed. An implantable loop recorder (ILR) was implanted at the end of the ablation in each patient. Results: Thirty-one consecutive patients (66 ± 8 years and 71% male) affected by PeAF were included in this study. The VOM-EI procedural phase lasted 21.4 ± 10.1 min. PV isolation and lines were validated in all subjects. The ML block was achieved within 10.8 ± 8.7 min. At a mean follow-up of 12 ± 7 months, 27 out of 31 (87%) patients remained free from AT/AF recurrences. Among the patients with recurrences, two (50%) had incomplete ablation lesions and three (75%) had “suboptimal” VOM-EI. In 23/31 patients (74%), antiarrhythmic drugs (AADs) were discontinued after 1 month of follow-up. No significant complications were reported during the follow-up. Conclusions: this single-center experience demonstrates that VOM-EI systematically combined with an anatomical ablation set in patients with PeAF resulted in feasible, safe, and effective freedom from AF/AT recurrences in 87% of the population after a 1-year follow-up period according to an ILR.
Constraints on Optimising Encoder-Only Transformers for Modelling Sign Language with Human Pose Estimation Keypoint Data
Authors: Woods Luke T. , Rana Zeeshan A.
Published: November 2023

Supervised deep learning models can be optimised by applying regularisation techniques to reduce overfitting, which can prove difficult when fine tuning the associated hyperparameters. Not all hyperparameters are equal, and understanding the effect each hyperparameter and regularisation technique has on the performance of a given model is of paramount importance in research. We present the first comprehensive, large-scale ablation study for an encoder-only transformer to model sign language using the improved Word-level American Sign Language dataset (WLASL-alt) and human pose estimation keypoint data, with a view to put constraints on the potential to optimise the task. We measure the impact a range of model parameter regularisation and data augmentation techniques have on sign classification accuracy. We demonstrate that within the quoted uncertainties, other than ℓ2 parameter regularisation, none of the regularisation techniques we employ have an appreciable positive impact on performance, which we find to be in contradiction to results reported by other similar, albeit smaller scale, studies. We also demonstrate that the model architecture is bounded by the small dataset size for this task over finding an appropriate set of model parameter regularisation and common or basic dataset augmentation techniques. Furthermore, using the base model configuration, we report a new maximum top-1 classification accuracy of 84% on 100 signs, thereby improving on the previous benchmark result for this model architecture and dataset.
Endophytic Fungal Diversity in Cirsium kawakamii from Taiwan
Authors: Chen Yi-Jeng , Chen Hui-Juan , Chung Wen-Hsin
Published: November 2023

The endophytic fungal diversity of Cirsium kawakamii, a herb indigenous to Taiwan, was analyzed in this study. In addition, some fungal isolates were evaluated for the risk they pose as plant pathogens. In total, 1836 endophytic fungi were isolated from C. kawakamii from Hehuanjian, Puli Township, and Tatachia. They were classified into 2 phyla, 8 classes, 40 families, and 68 genera. Colletotrichum, Fusarium, Phomopsis, and Xylaria, (Ascomycota, Sordariomycetes) were the dominant genera. The genus accumulation curve (based on the bootstrap estimator) was non-asymptotic, with estimated richness significantly exceeding the richness captured by our sampling to date. Considering the collection time, the data indicated significant differences in the proportions of the C. kawakamii endophyte genus from Hehuanjan, Puli Township (across two seasons), and Tatachia. The Shannon and Gini–Simpson indices revealed variations in diversity, with C. kawakamii endophytes (Puli Township in winter) significantly reducing alpha diversity compared with other seasons and locations. Meanwhile, the Gini–Simpson index suggested that there were no significant differences in richness among the four sampling sites. The PCA results unveiled distinct community structures across different locations and seasons, explaining 46.73% of the total variation in fungal community composition significantly affected diversity and richness. In addition, a considerable number of Fusarium isolates exhibited harmful properties towards wheat, potatoes, and apples. It is postulated that these fungi belong to the Fusarium tricinctum species complex (FTSC).
48-Month Clinical Outcomes and Prognostic Factors in an All-Comers Population with Acute Coronary Syndrome and Chronic Coronary Syndrome Undergoing Percutaneous Coronary Intervention with a Sirolimus-Eluting Stent
Authors: Tyczynski Maciej , Kern Adam , Buller Patryk , Gil Robert J. , Bil Jacek
Published: November 2023

We characterized the performance as well as safety of a second-generation thin-strut sirolimus-eluting stent with a biodegradable polymer, Alex Plus (Balton, Poland), deployed in the acute coronary syndrome (ACS) setting. We enrolled patients who were subjected to percutaneous coronary intervention (PCI) between July 2015 and March 2016 and took into consideration demographics, clinical and laboratory data, and clinical outcomes. We defined the primary endpoint as the 48-month rate of major cardiovascular adverse events (MACE), including cardiac death, myocardial infarction (MI), or target lesion revascularization (TLR). The secondary endpoints were all-cause death, cardiac death, MI, and TLR rates at 12-, 24-, 36-, and 48 months. We enrolled 232 patients in whom 282 stents were implanted, including 88 ACS and 144 chronic coronary syndrome (CCS) patients. The mean age of the ACS population was 67 ± 13 years old, and 32% of it consisted of females. Patients with ACS were characterized by lower rates of arterial hypertension (85.2% vs. 95.8%, p = 0.004), dyslipidemia (67% vs. 81.9%, p = 0.01), prior MI (34.1% vs. 57.6%, p < 0.001), and prior PCI (35.2% vs. 68.8%, p < 0.001). At 48 months, among the ACS patients, the rates of MACE, death, cardiac death, MI, and TLR were 23.9%, 11.4%, 7.9%, 9.1%, and 10.2%, respectively. No stent thrombosis cases were reported. Multivariable Cox regression revealed that the statistically significant MACE predictors were massive calcifications in coronary arteries (HR 9.0, 95% CI 1.75–46.3, p = 0.009), post-dilatation (HR 3.78, 95% CI 1.28–11.2, p = 0.016), prior CABG (HR 6.64, 95% CI 1.62–27.1, p = 0.008), vitamin K antagonist use (HR 5.99, 95% CI 1.29–27.8, p = 0.022), and rivaroxaban use (HR 51.7, 95% CI 4.48–596, p = 0.002). The study findings show that Alex Plus was effective and safe in a contemporary cohort of real-world ACS patients undergoing primary PCI. The outcomes were comparable between the ACS and chronic coronary syndrome patients, with a trend of lower TLR in ACS patients at 4 years.