bluuebunny/crossref_metadata_2025 huggingface dataset:
An Improved Discrete Bat Algorithm for Multi-Objective Partial Parallel Disassembly Line Balancing Problem
Authors: Zhang Qi , Xing Yang , Yao Man , Wang Jiacun , Guo Xiwang , Qin Shujin , Qi Liang , Huang Fuguang
Published: February 2024

Product disassembly is an effective means of waste recycling and reutilization that has received much attention recently. In terms of disassembly efficiency, the number of disassembly skills possessed by workers plays a crucial role in improving disassembly efficiency. Therefore, in order to effectively and reasonably disassemble discarded products, this paper proposes a partial parallel disassembly line balancing problem (PP-DLBP) that takes into account the number of worker skills. In this paper, the disassembly tasks and the disassembly relationships between components are described using AND–OR graphs. In this paper, a multi-objective optimization model is established aiming to maximize the net profit of disassembly and minimize the number of skills for the workers. Based on the bat algorithm (BA), we propose an improved discrete bat algorithm (IDBA), which involves designing adaptive composite optimization operators to replace the original continuous formula expressions and applying them to solve the PP-DLBP. To demonstrate the advantages of IDBA, we compares it with NSGA-II, NSGA-III, SPEA-II, ESPEA, and MOEA/D. Experimental results show that IDBA outperforms the other five algorithms in real disassembly cases and exhibits high efficiency.
A Stabilisation System Synthesis for Motion along a Preset Trajectory and Its Solution by Symbolic Regression
Authors: Diveev Askhat , Sofronova Elena , Konyrbaev Nurbek
Published: February 2024

The problem of a stabilisation system synthesis for the motion of a control object along a given spatial trajectory is considered. The complexity of the problem is that the preset trajectory is defined in the state subspace and not in time. This paper describes a stabilisation system synthesis for motion along a trajectory specified in time and along a trajectory specified in the form of a manifold in a state space. In order to construct a stabilisation system, it is necessary to determine a distance between an object and the given trajectory at each moment in time. For trajectories that are not given in time, the determination of this distance can be ambiguous. An object may be exactly on a trajectory but at a different time. This paper proposes some approaches to solve the problem. One of the approaches is to transform a given trajectory in a state subspace into a trajectory given in time. A description of a universal method to perform this transformation is presented. In order to solve the synthesis problem automatically, without having to analyse the mathematical model of the control object, it is suggested that machine learning control by symbolic regression is used. In computational experiments, examples of stabilisation system syntheses for quadcopter motion along a given spatial trajectory are presented.
Dimensionless Policies Based on the Buckingham π Theorem: Is This a Good Way to Generalize Numerical Results?
Authors: Girard Alexandre
Published: February 2024

The answer to the question posed in the title is yes if the context (the list of variables defining the motion control problem) is dimensionally similar. This article explores the use of the Buckingham π theorem as a tool to encode the control policies of physical systems into a more generic form of knowledge that can be reused in various situations. This approach can be interpreted as enforcing invariance to the scaling of the fundamental units in an algorithm learning a control policy. First, we show, by restating the solution to a motion control problem using dimensionless variables, that (1) the policy mapping involves a reduced number of parameters and (2) control policies generated numerically for a specific system can be transferred exactly to a subset of dimensionally similar systems by scaling the input and output variables appropriately. Those two generic theoretical results are then demonstrated, with numerically generated optimal controllers, for the classic motion control problem of swinging up a torque-limited inverted pendulum and positioning a vehicle in slippery conditions. We also discuss the concept of regime, a region in the space of context variables, that can help to relax the similarity condition. Furthermore, we discuss how applying dimensional scaling of the input and output of a context-specific black-box policy is equivalent to substituting new system parameters in an analytical equation under some conditions, using a linear quadratic regulator (LQR) and a computed torque controller as examples. It remains to be seen how practical this approach can be to generalize policies for more complex high-dimensional problems, but the early results show that it is a promising transfer learning tool for numerical approaches like dynamic programming and reinforcement learning.
Existence of Traveling Waves of a Diffusive Susceptible–Infected–Symptomatic–Recovered Epidemic Model with Temporal Delay
Authors: Miranda Julio C. , Arenas Abraham J. , González-Parra Gilberto , Villada Luis Miguel
Published: February 2024

The aim of this article is to investigate the existence of traveling waves of a diffusive model that represents the transmission of a virus in a determined population composed of the following populations: susceptible (S), infected (I), asymptomatic (A), and recovered (R). An analytical study is performed, where the existence of solutions of traveling waves in a bounded domain is demonstrated. We use the upper and lower coupled solutions method to achieve this aim. The existence and local asymptotic stability of the endemic (Ee) and disease-free (E0) equilibrium states are also determined. The constructed model includes a discrete-time delay that is related to the incubation stage of a virus. We find the crucial basic reproduction number R0, which determines the local stability of the steady states. We perform numerical simulations of the model in order to provide additional support to the theoretical results and observe the traveling waves. The model can be used to study the dynamics of SARS-CoV-2 and other viruses where the disease evolution has a similar behavior.
A Class of Power Series q-Distributions
Authors: Charalambides Charalambos A.
Published: February 2024

A class of power series q-distributions, generated by considering a q-Taylor expansion of a parametric function into powers of the parameter, is discussed. Its q-factorial moments are obtained in terms of q-derivatives of its series (parametric) function. Also, it is shown that the convolution of power series q-distributions is also a power series q-distribution. Furthermore, the q-Poisson (Heine and Euler), q-binomial of the first kind, negative q-binomial of the second kind, and q-logarithmic distributions are shown to be members of this class of distributions and their q-factorial moments are deduced. In addition, the convolution properties of these distributions are examined.
On Efficient Parallel Secure Outsourcing of Modular Exponentiation to Cloud for IoT Applications
Authors: Rath Satyabrat , Ramalingam Jothi , Lee Cheng-Chi
Published: February 2024

Modular exponentiation is crucial for secure data exchange in cryptography, especially for resource-constrained Internet of Things (IoT) devices. These devices often rely on third-party servers to handle computationally intensive tasks like modular exponentiation. However, existing outsourcing solutions for the RSA algorithm may have security vulnerabilities. This work identifies a critical flaw in a recent outsourcing protocol for RSA proposed by Hu et al. We demonstrate how this flaw compromises the security of the entire RSA system. Subsequently, we propose a robust solution that strengthens the RSA algorithm and mitigates the identified vulnerability. Furthermore, our solution remains resilient against existing lattice-based attacks. The proposed fix offers a more secure and efficient way for IoT devices to leverage the power of third-party servers while maintaining data integrity and confidentiality. An extensive performance evaluation confirms that our solution offers comparable efficiency while significantly enhancing security compared to existing approaches.
Advancing the Social Internet of Things (SIoT): Challenges, Innovations, and Future Perspectives
Authors: Hosseinzadeh Mehdi , Mohammadi Venus , Lansky Jan , Nulicek Vladimir
Published: February 2024

This study conducts an in-depth review of the Social Internet of Things (SIoT), a significant advancement from the conventional Internet of Things (IoT) via the integration of socialization principles akin to human interactions. We explore the architecture, trust management, relationship dynamics, and other crucial aspects of SIoT, with a particular focus on the relatively neglected areas of fault tolerance, cloud–fog computing, and clustering. Our systematic literature analysis, spanning research from 2011 to April 2023, uncovers critical gaps and establishes a detailed taxonomy of emerging SIoT themes. This paper not only sheds light on the current state of SIoT research but also charts a course for future exploration and development in this burgeoning field.
Gender Disparities in Health Biomarkers, Lifestyle Patterns, and Nutritional Status among Bank Staff: A Cross-Sectional Study
Authors: Schauer Markus , Burtscher Martin , Motevalli Mohamad , Tanous Derrick , Mair Susanne , Wirnitzer Katharina
Published: February 2024

Background and Objectives: Despite the importance of nutritional status and a healthy lifestyle in shaping overall well-being, little is known about examining gender-specific differences and trends in health, lifestyle, and nutritional status. The present study aimed to evaluate blood levels of micronutrients, homocysteine, and CoQ10, as well as physical activity (PA) levels and sedentary behavior, among a cohort of Austrian bank staff, with a particular focus on identifying gender differences as well as gender-specific nutritional deficiencies compared to the reference ranges. Materials and Methods: Following a cross-sectional study design, 123 Austrian bank staff (mean age: 43 years; 51% females) participated in this study. Blood samples were collected to evaluate participants’ micronutrient status and serum levels of homocysteine and CoQ10. Whole-blood values of macronutrients were compared to gender-specific reference ranges and categorized into three groups: below, within, or over the range. The WHO’s Global Physical Activity Questionnaire was used to assess PA levels and sedentary behaviors. Results: No significant difference between males and females was found for diet types, PA levels, sedentary time, homocysteine levels, or CoQ10 values (p > 0.05). A high PA level was reported by 64% of males and 58% of females. 71% of females and 56% of males were found to have a vitamin D deficiency. 63–98% of females and 72–97% of males showed normal blood levels for the remaining micronutrients, including potassium, calcium, magnesium, copper, iron, zinc, selenium, manganese, molybdenum, B6, B9, and B12. Conclusions: The findings highlight the necessity of implementing tailored strategies to foster healthy lifestyle behaviors, thereby enhancing the overall state of health, particularly in the context of occupational health.
Influence of the Applied External Magnetic Field on the Deposition of Ni–Cu Alloys
Authors: Skibińska Katarzyna , Elsharkawy Safya , Kołczyk-Siedlecka Karolina , Kutyła Dawid , Żabiński Piotr
Published: February 2024

Ni–Cu alloys are suitable candidates as catalysts in hydrogen evolution reaction. Because of the different magnetic properties of Ni and Cu, the influence of an applied external magnetic field on the synthesis Ni–Cu alloys was studied. The coatings were prepared with visible changes in their appearance. The differences between the observed regions were studied in terms of morphology and chemical composition. In addition, the overall chemical and phase compositions were determined using X-ray fluorescence and X-ray diffraction methods, respectively. The catalytic activity was measured in 1 M NaOH using linear sweep voltammetry. The contact angle was determined using contour analysis. All samples were hydrophilic. Hydrogen evolution started at different times depending on the area on the surface. It started earliest on the coating obtained in parallel to the electrode magnetic field at 250 mT. We found that when the Lorenz force is maximal, Cu deposition is preferred because of the enhancement of mass transport.
Automated Spectrophotometric Assays for the Measurement of Ammonia and Bicarbonate in Saliva of Horses: Analytical Validation and Changes in Equine Gastric Ulcer Syndrome (EGUS)
Authors: Muñoz-Prieto Alberto , Llamas-Amor Eva , Contreras-Aguilar María Dolores , Ayala Ignacio , Martín Cuervo María , Cerón José Joaquín , Hansen Sanni
Published: February 2024

Ammonia (NH3) and bicarbonate (HCO3) have been related to gastric ulcers in humans. Ammonia is considered a possible cause of gastric ulcers, whereas bicarbonate has a protective function. The presence of ulcers in the stomach of horses is defined as Equine Gastric Ulcer Syndrome (EGUS), which is a frequent disease in this species, and it has been associated with changes in saliva composition, such as in analytes related to inflammation, immune system and oxidative stress. The objectives of this study were (1) to perform an analytical validation of two automated spectrophotometric assays, one for ammonia and one for bicarbonate, in the horses’ saliva and (2) to evaluate their possible variations with EGUS. Analytical validation of the automated assays for ammonia and bicarbonate in the saliva of horses showed that both assays were precise and accurate. In addition, significantly higher values of ammonia and lower values of bicarbonate were found in the saliva of horses with EGUS compared to healthy horses. It can be concluded that ammonia and bicarbonate can be measured in the saliva of horses and that ammonia increases and bicarbonate decreases in this sample type could be related to the presence of EGUS in this species.
Unveiling the Mutations and Conservation of InlA in Listeria monocytogenes
Authors: Li Lingling , Wang Yan , Pu Ji , Chen Jinni , Liu Lingyun , Mao Pan , Sun Hui , Luo Xia , Ye Changyun
Published: February 2024

Listeria monocytogenes (L. monocytogenes) is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of L. monocytogenes into the human intestinal epithelium. In addition, InlA enhances the pathogenicity of host strains, and different strains of L. monocytogenes contain varying variations of InlA. Our study analyzed a total of 4393 published L. monocytogenes genomes from 511 sequence types (STs) of diverse origins. We identified 300 unique InlA protein sequence types (PSTs) and revealed 45 highly mutated amino acid sites. The leucine-rich repeat (LRR) region was found to be the most conserved among the InlA, while the protein A (PA) region experienced the highest mutation rate. Two new types of mutations were identified in the B-repeat region of InlA. Correspondence analysis (CA) was used to analyze correlations between the lineages or 10 most common sequence types (STs) and amino acid (aa) sites. ST8 was strongly correlated with site 192_F, 454_T. ST7 exhibited a strong correlation with site 51_A, 573_E, 648_S, and 664_A, and it was also associated with ST6 and site 544_N, 671_A, 738_B, 739_B, 740_B, and 774_Y. Additionally, a strong correlation between ST1 and site 142_S, 738_N, ST2 and site 2_K, 142_S, 738_N, as well as ST87 and site2_K, 738_N was demonstrated. Our findings contribute significantly to the understanding of the distribution, composition, and conservation of InlA in L. monocytogenes. These findings also suggest a potential role of InlA in supporting molecular epidemiological tracing efforts.
Differential Microbial Composition and Interkingdom Interactions in the Intestinal Microbiota of Holstein and German Simmental × Holstein Cross F1 Calves: A Comprehensive Analysis of Bacterial and Fungal Diversity
Authors: Nan Shanshan , Li Jiacheng , Kuang Yu , Feng Jiaqi , Wang Hailiang , Niu Junli , Wu Yanyan , Zhang Wenju , Nie Cunxi
Published: February 2024

Calf intestines are colonized by rich and complex microbial communities, playing a crucial role in animal physiology, metabolism, nutrition, and immune function. In this study, we provide insight into the composition of fecal microbial bacteria and fungi, respectively, as well as the cross-kingdom interactions. We investigated the intestinal microbiota of different breeds of calves by characterizing the bacterial and fungal communities in the rectal feces of Holstein calves and German Simmental × Holstein cross F1 generation (GXH) using 16S rRNA and ITS amplicon sequencing techniques. PICRUSt2 (version 2.2.0) were used to determine microbial diversity and function and explore the reasons why Holstein calves are more susceptible to diarrhea. The results revealed no significant difference in the diversity of fecal microbiota among the groups (p > 0.05). We identified Firmicutes, Bacteroidetes, and Proteobacteria as the dominant bacterial phyla in the fecal bacterial communities of the two breeds of calves. Ascomycota and Basidiomycota play important roles in the fungal community but differ in relative abundance. Bacteroides was the dominant genus at the group level for calf fecal microbiota in both breeds. The relative abundance of Prevotella, Escherichia-Shigella, Peptostreptococcus, and Butyricicoccus was higher in Holstein calves, and the relative abundance of Faecalibacterium, Megamonas, Butyricicoccus, and Alloprevotella was lower than GXH group. Aspergillus and Cladosporium were the dominating genera of fecal fungi in both groups of calves. LEfSe analysis revealed 33 different bacteria and 23 different fungi between the two groups, with more differential strains found in GXH. In addition, the feces fungi-bacteria interkingdom interactions varied among breeds. Thus, the composition and structure of bacterial and fungal communities in calf feces varied by breed, indicating a potential association between breed and microbial communities. We also found differences in the network between bacterial–fungal kingdoms. We explain the reasons for Holstein calves being more prone to diarrhea. This indicated that breed makes differences in calf diarrhea rates by influencing gut microbial composition and interactions.
Balancing Act: Tubulin Glutamylation and Microtubule Dynamics in Toxoplasma gondii
Authors: Delgado Inês L. S. , Gonçalves João , Fernandes Rita , Zúquete Sara , Basto Afonso P. , Leitão Alexandre , Soares Helena , Nolasco Sofia
Published: February 2024

The success of the intracellular parasite Toxoplasma gondii in invading host cells relies on the apical complex, a specialized microtubule cytoskeleton structure associated with secretory organelles. The T. gondii genome encodes three isoforms of both α- and β-tubulin, which undergo specific post-translational modifications (PTMs), altering the biochemical and biophysical proprieties of microtubules and modulating their interaction with associated proteins. Tubulin PTMs represent a powerful and evolutionarily conserved mechanism for generating tubulin diversity, forming a biochemical ‘tubulin code’ interpretable by microtubule-interacting factors. T. gondii exhibits various tubulin PTMs, including α-tubulin acetylation, α-tubulin detyrosination, Δ5α-tubulin, Δ2α-tubulin, α- and β-tubulin polyglutamylation, and α- and β-tubulin methylation. Tubulin glutamylation emerges as a key player in microtubule remodeling in Toxoplasma, regulating stability, dynamics, interaction with motor proteins, and severing enzymes. The balance of tubulin glutamylation is maintained through the coordinated action of polyglutamylases and deglutamylating enzymes. This work reviews and discusses current knowledge on T. gondii tubulin glutamylation. Through in silico identification of protein orthologs, we update the recognition of putative proteins related to glutamylation, contributing to a deeper understanding of its role in T. gondii biology.
Nanoparticles and Their Biological Applications: Recent Advances in 2022–2023
Authors: Zaman Wajid , Manghwar Hakim
Published: February 2024

This Special Issue illustrates the collaborative effort required to harness the potential of nanoparticles, showcasing their role in targeting drug-resistant bacteria and highlighting new pathways for drug delivery, diagnostics, and beyond [...]
Emergence of Recombinant Subclade D3/Y in Coxsackievirus A6 Strains in Hand-Foot-and-Mouth Disease (HFMD) Outbreak in India, 2022
Authors: Tikute Sanjaykumar , Deshmukh Pratik , Chavan Nutan , Shete Anita , Shinde Pooja , Yadav Pragya , Lavania Mallika
Published: February 2024

Coxsackievirus-A6 (CV-A6) is responsible for more severe dermatological manifestations compared to other enteroviruses such as CV-A10, CV-A16, and EV-A71, causing HFMD in children and adults. Between 2005 and 2007, the recombinant subclade D3/RF-A started to expand globally, and a CV-A6 pandemic started. The study aimed to conduct whole-genome sequencing (WGS) of an isolated CV-A6 strain from currently circulating HFMD cases from India in 2022. Gene-specific RT-PCR and sequencing were used to perform molecular characterization of the isolated virus. Confirmation of these isolates was also performed by transmission electron microscopy and WGS. Among eleven positive clinical enterovirus specimens, eight CV-A6 strains were successfully isolated in the RD cell line. Isolates confirmed the presence of the CV-A6 strain based on VP1 and VP2 gene-specific RT-PCR. Sequences of isolates were clustered and identified as the novel CV-A6 strain of the D3/Y sub-genotype in India. The studies revealed that the D3/Y sub-genotype is being introduced into Indian circulation. The predicted putative functional loops found in VP1 of CV-A6 showed that the nucleotide sequences of the amino acid were a remarkably conserved loop prediction compatible with neutralizing linear epitopes. Therefore, this strain represents a potential candidate for vaccine development and antiviral studies.
Deformation and Transformation Textures in the NaMgF3 Neighborite—Post-Perovskite System
Authors: Ledoux Estelle E. , Jugle Michael , Stackhouse Stephen , Miyagi Lowell
Published: February 2024

The D″ region of the lower mantle, which lies just above the core–mantle boundary, is distinct from the bulk of the lower mantle in that it exhibits complex seismic heterogeneity and seismic anisotropy. Seismic anisotropy in this region is likely to be largely due to the deformation-induced texture (crystallographic preferred orientation) development of the constituent mineral phases. Thus, seismic anisotropy can provide a marker for deformation processes occurring in this dynamic region of the Earth. Post-perovskite-structured (Mg,Fe)SiO3 is believed to be the dominant mineral phase in many regions of the D”. As such, understanding deformation mechanisms and texture development in post-perovskite is important for the interpretation of observed seismic anisotropy. Here, we report on high-pressure diamond anvil cell deformation experiments on NaMgF3 neighborite (perovskite structure) and post-perovskite. During deformation, neighborite develops a 100 texture, as has been previously observed, both in NaMgF3 and MgSiO3 perovskite. Upon transformation to the post-perovskite phase, an initial texture of {130} at high angles to compression is observed, indicating that the {100} planes of perovskite become the ~{130} planes of post-perovskite. Further compression results in the development of a shoulder towards (001) in the inverse pole figure. Plasticity modeling using the elasto-viscoplastic self-consistent code shows this texture evolution to be most consistent with deformation on (001)[100] with some contribution of glide on (100)[010] and (001)<110> in NaMgF3 post-perovskite. The transformation and deformation mechanisms observed in this study in the NaMgF3 system are consistent with the behavior generally observed in other perovskite–post-perovskite systems, including the MgSiO3 system. This shows that NaMgF3 is a good analog for the mantle bridgmanite and MgSiO3 post-perovskite.
Transformation of the Geological Environment under the Influence of Liquid Radioactive Waste (Russian Experience in Studying Historical Nuclear Disposal)
Authors: Krupskaya Victoria , Zakusin Sergey , Chernov Mikhail
Published: February 2024

Due to various historical events, in the Russian Federation, in addition to the radioactive waste storage facilities used in world practice, there are various nuclear and radiation hazardous facilities that require special procedures for monitoring and decommissioning. One of these facilities is the disposal site for LRW on the territory of the JSC Siberian Chemical Plant, where specially prepared waste is injected into sand reservoirs lying at depths of 300–350 m between clayey strata. This study examines in detail the features of the lithological and mineral composition of reservoir sands and aquitards. The processes of environmental transformation in reservoir sands, which lead to changes in the composition and structure of rocks, were characterized. These processes manifest themselves in the form of the development of leaching zones and their “healing” with newly formed smectite, the destruction of terrigenous grains, including the development of cracks, and the growth of newly formed smectite in the pore space of reservoirs. The forms of occurrence and localization of authigenic smectite formed as a result of technogenic impact are described. It has been shown that, despite the obvious impact of highly reactive solutions accompanying liquid radioactive waste, the insulating properties of the geological environment are maintained and even improved to some extent.
Supramolecular Switch for the Regulation of Antibacterial Efficacy of Near-Infrared Photosensitizer
Authors: Jiang Yu-Na , Tan Manqi , He Chenglong , Wang Jiaxi , Wei Yi , Jing Ningning , Wang Bing , Yang Fang , Zhang Yujie , Li Meng
Published: February 2024

The global antibiotic resistance crisis has drawn attention to the development of treatment methods less prone to inducing drug resistance, such as antimicrobial photodynamic therapy (aPDT). However, there is an increasing demand for new photosensitizers capable of efficiently absorbing in the near-infrared (NIR) region, enabling antibacterial treatment in deeper sites. Additionally, advanced strategies need to be developed to avert drug resistance stemming from prolonged exposure. Herein, we have designed a conjugated oligoelectrolyte, namely TTQAd, with a donor-acceptor-donor (D-A-D) backbone, enabling the generation of reactive oxygen species (ROS) under NIR light irradiation, and cationic adamantaneammonium groups on the side chains, enabling the host-guest interaction with curcubit[7]uril (CB7). Due to the amphiphilic nature of TTQAd, it could spontaneously form nanoassemblies in aqueous solution. Upon CB7 treatment, the positive charge of the cationic adamantaneammonium group was largely shielded by CB7, leading to a further aggregation of the nanoassemblies and a reduced antibacterial efficacy of TTQAd. Subsequent treatment with competitor guests enables the release of TTQAd and restores its antibacterial effect. The reversible supramolecular switch for regulating the antibacterial effect offers the potential for the controlled release of active photosensitizers, thereby showing promise in preventing the emergence of drug-resistant bacteria.
Novel 4-Chromanone-Derived Compounds as Plant Immunity Inducers against CMV Disease in Passiflora spp. (Passion Fruit)
Authors: Wu Tianli , Yu Lu , Xiao Lingling , Wang Tao , Li Pei , Mu Bo
Published: February 2024

This study involved the design and synthesis of a series of novel 4-chromanone-derived compounds. Their in vivo anti-cucumber mosaic virus (CMV) activity in field trials against CMV disease in Passiflora spp. was then assessed. Bioassay results demonstrated that compounds 7c and 7g exhibited remarkable curative effects and protection against CMV, with inhibition rates of 57.69% and 51.73% and 56.13% and 52.39%, respectively, surpassing those of dufulin and comparable to ningnanmycin. Field trials results indicated that compound 7c displayed significant efficacy against CMV disease in Passiflora spp. (passion fruit) after the third spraying at a concentration of 200 mg/L, with a relative control efficiency of 47.49%, surpassing that of dufulin and comparable to ningnanmycin. Meanwhile, nutritional quality test results revealed that compound 7c effectively enhanced the disease resistance of Passiflora spp., as evidenced by significant increases in soluble protein, soluble sugar, total phenol, and chlorophyll contents in Passiflora spp. leaves as well as improved the flavor and taste of Passiflora spp. fruits, as demonstrated by notable increases in soluble protein, soluble sugar, soluble solid, and vitamin C contents in Passiflora spp. fruits. Additionally, a transcriptome analysis revealed that compound 7c primarily targeted the abscisic acid (ABA) signaling pathway, a crucial plant hormone signal transduction pathway, thereby augmenting resistance against CMV disease in Passiflora spp. Therefore, this study demonstrates the potential application of these novel 4-chromanone-derived compounds as effective inducers of plant immunity for controlling CMV disease in Passiflora spp. in the coming decades.
Synthesis, Structural Characterization, Hirschfeld Surface Analysis, Density Functional Theory, and Photocatalytic CO2 Reduction Activity of a New Ca(II) Complex with a Bis-Schiff Base Ligand
Authors: Tai Xishi , Yan Xihai , Wang Lihua
Published: February 2024

A new bis-Schiff base (L) Ca(II) complex, CaL, was synthesized by the reaction of calcium perchlorate tetrahydrate, 1,3-diamino-2-hydroxypropane, and 2-formyl phenoxyacetic acid in an ethanol–water (v:v = 2:1) solution and characterized by IR, UV-vis, TG-DTA, and X-ray single crystal diffraction analysis. The structural analysis indicates that the Ca(II) complex crystallizes in the monoclinic system, space group P121/n1, and the Ca(II) ions are six-coordinated with four O atoms (O8, O9, O11, O12, or O1, O2, O4, O6) and two N atoms (N1, N2, or N3, N4) of one bis-Schiff base ligand. The Ca(II) complex forms a tetramer by intermolecular O-H…O hydrogen bonds. The tetramer units further form a three-dimensional network structure by π–π stacking interactions of benzene rings. The Hirschfeld surface of the Ca(II) complex shows that the H…H contacts represent the largest contribution (41.6%) to the Hirschfeld surface, followed by O…H/H…O and C…H/H…C contacts with contributions of 35.1% and 18.1%, respectively. To understand the electronic structure of the Ca(II) complex, the DFT calculations were carried out. The photocatalytic CO2 reduction test of the Ca(II) complex exhibited a yield of 47.9 μmol/g (CO) and a CO selectivity of 99.3% after six hours.
Optimized Conditions for the Extraction of Phenolic Compounds from Aeginetia indica L. and Its Potential Biological Applications
Authors: On-Nom Nattira , Thangsiri Sirinapa , Inthachat Woorawee , Temviriyanukul Piya , Sahasakul Yuraporn , Aursalung Amornrat , Chupeerach Chaowanee , Suttisansanee Uthaiwan
Published: February 2024

Aeginetia indica L., a parasitic root in the Orobanchaceae family, is used as a food colorant in traditional Thai desserts. However, scant information is available on its food applications as well as medicinal properties, while overharvesting by the local people has severely depleted wild plant populations. This research, thus, aimed to extract optimized total phenolic content (TPC) in varying extraction conditions using response surface methodology (RSM) and the Box–Behnken design (BBD). Results indicated that an extraction temperature of 90 °C, 80% (v/v) aqueous ethanol, and 0.5% (w/v) solid-to-liquid ratio yielded the highest TPC at 129.39 mg gallic acid equivalent (GAE)/g dry weight (DW). Liquid chromatography–electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified the predominant phenolics as apigenin (109.06 mg/100 g extract) and luteolin (35.32 mg/100 g extract) with trace amounts of naringenin and rutin. Under the optimal extraction condition, the plant extract exhibited antioxidant activities of 5620.58 and 641.52 µmol Trolox equivalent (TE)/g DW determined by oxygen radical absorbance capacity (ORAC) and ferric ion reducing antioxidant power (FRAP) assay, while the scavenging capacity of total radicals at 50% (SC50) was determined to be 135.50 µg/mL using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The plant extract also exhibited inhibitory activities against the key enzymes relevant to type II diabetes, obesity, and Alzheimer’s disease, suggesting the potential for medicinal applications.
LC-MS-Based Metabolomics Reveals the Mechanism of Protection of Berberine against Indomethacin-Induced Gastric Injury in Rats
Authors: Xu Jing , Yang Xiu-Wei
Published: February 2024

Berberine is a natural isoquinoline alkaloid with low toxicity, which exists in a wide variety of medicinal plants. Berberine has been demonstrated to exhibit potent prevention of indomethacin-induced gastric injury (GI) but the related mechanism remains unclear. In the present study, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics was applied for the first time to investigate the alteration of serum metabolites in the protection of berberine against indomethacin-induced gastric injury in rats. Subsequently, bioinformatics was utilized to analyze the potential metabolic pathway of the anti-GI effect of berberine. The pharmacodynamic data indicated that berberine could ameliorate gastric pathological damage, inhibit the level of proinflammatory factors in serum, and increase the level of antioxidant factors in serum. The LC-MS-based metabolomics analysis conducted in this study demonstrated the presence of 57 differential metabolites in the serum of rats with induced GI caused by indomethacin, which was associated with 29 metabolic pathways. Moreover, the study revealed that berberine showed a significant impact on the differential metabolites, with 45 differential metabolites being reported between the model group and the group treated with berberine. The differential metabolites were associated with 24 metabolic pathways, and berberine administration regulated 14 of the 57 differential metabolites, affecting 14 of the 29 metabolic pathways. The primary metabolic pathways affected were glutathione metabolism and arachidonic acid metabolism. Based on the results, it can be concluded that berberine has a gastroprotective effect on the GI. This study is particularly significant since it is the first to elucidate the mechanism of berberine’s action on GI. The results suggest that berberine’s action may be related to energy metabolism, oxidative stress, and inflammation regulation. These findings may pave the way for the development of new therapeutic interventions for the prevention and management of NSAID-induced GI disorders.
Electronic Skin Based on Polydopamine-Modified Superelastic Fibers with Superior Conductivity and Durability
Authors: Chen Chengfeng , Wang Yimiao , Wang Hang , Wang Xinqing , Tian Mingwei
Published: February 2024

Owing to their excellent elasticities and adaptability as sensing materials, ionic hydrogels exhibit significant promise in the field of intelligent wearable devices. Nonetheless, molecular chains within the polymer network of hydrogels are susceptible to damage, leading to crack extension. Hence, we drew inspiration from the composite structure of the human dermis to engineer a composite hydrogel, incorporating dopamine-modified elastic fibers as a reinforcement. This approach mitigates crack expansion and augments sensor sensitivity by fostering intermolecular forces between the dopamine on the fibers, the hydrogel backbone, and water molecules. The design of this composite hydrogel elevates its breaking tensile capacity from 35 KJ to 203 KJ, significantly enhancing the fatigue resistance of the hydrogel. Remarkably, its electrical properties endure stability even after 2000 cycles of testing, and it manifests heightened sensitivity compared to conventional hydrogel configurations. This investigation unveils a novel method for crafting composite-structured hydrogels.
Wide Response Range Photoelectrochemical UV Detector Based on Anodized TiO2-Nanotubes@Ti@quartz Structure
Authors: Wang Youqing , Zhang Miaomiao , Wu Wenxuan , Wang Ze , Liu Minghui , Yang Tiantian , Renqianzhuoma
Published: February 2024

Conventional sandwich structure photoelectrochemical UV detectors cannot detect UV light below 300 nm due to UV filtering problems. In this work, we propose to place the electron collector inside the active material, thus avoiding the effect of electrodes on light absorption. We obtained a TiO2-nanotubes@Ti@quartz photoanode structure by precise treatment of a commercial Ti mesh by anodic oxidation. The structure can absorb any light in the near-UV band and has superior stability to other metal electrodes. The final encapsulated photoelectrochemical UV detectors exhibit good switching characteristics with a response time below 100 ms. The mechanism of the oxidation conditions on the photovoltaic performance of the device was investigated by the electrochemical impedance method, and we obtained the optimal synthesis conditions. Response tests under continuous spectroscopy confirm that the response range of the device is extended from 300–400 nm to 240–400 nm. This idea of a built-in collector is an effective way to extend the response range of a photoelectrochemical detector.
The Effect of Stereocomplexation and Crystallinity on the Degradation of Polylactide Nanoparticles
Authors: Yin Chuan , Hemstedt Jenny , Scheuer Karl , Struczyńska Maja , Weber Christine , Schubert Ulrich S. , Bossert Jörg , Jandt Klaus D.
Published: February 2024

Polymeric nanoparticles (PNPs) are frequently researched and used in drug delivery. The degradation of PNPs is highly dependent on various properties, such as polymer chemical structure, size, crystallinity, and melting temperature. Hence, a precise understanding of PNP degradation behavior is essential for optimizing the system. This study focused on enzymatic hydrolysis as a degradation mechanism by investigation of the degradation of PNP with various crystallinities. The aliphatic polyester polylactide ([C3H4O2]n, PLA) was used as two chiral forms, poly l-lactide (PlLA) and poly d-lactide (PdLA), and formed a unique crystalline stereocomplex (SC). PNPs were prepared via a nanoprecipitation method. In order to further control the crystallinity and melting temperatures of the SC, the polymer poly(3-ethylglycolide) [C6H8O4]n (PEtGly) was synthesized. Our investigation shows that the PNP degradation can be controlled by various chemical structures, crystallinity and stereocomplexation. The influence of proteinase K on PNP degradation was also discussed in this research. AFM did not reveal any changes within the first 24 h but indicated accelerated degradation after 7 days when higher EtGly content was present, implying that lower crystallinity renders the particles more susceptible to hydrolysis. QCM-D exhibited reduced enzyme adsorption and a slower degradation rate in SC-PNPs with lower EtGly contents and higher crystallinities. A more in-depth analysis of the degradation process unveiled that QCM-D detected rapid degradation from the outset, whereas AFM exhibited delayed changes of degradation. The knowledge gained in this work is useful for the design and creation of advanced PNPs with enhanced structures and properties.
Apatite/Chitosan Composites Formed by Cold Sintering for Drug Delivery and Bone Tissue Engineering Applications
Authors: Galotta Anna , Demir Öznur , Marsan Olivier , Sglavo Vincenzo M. , Loca Dagnija , Combes Christèle , Locs Janis
Published: February 2024

In the biomedical field, nanocrystalline hydroxyapatite is still one of the most attractive candidates as a bone substitute material due to its analogies with native bone mineral features regarding chemical composition, bioactivity and osteoconductivity. Ion substitution and low crystallinity are also fundamental characteristics of bone apatite, making it metastable, bioresorbable and reactive. In the present work, biomimetic apatite and apatite/chitosan composites were produced by dissolution–precipitation synthesis, using mussel shells as a calcium biogenic source. With an eye on possible bone reconstruction and drug delivery applications, apatite/chitosan composites were loaded with strontium ranelate, an antiosteoporotic drug. Due to the metastability and temperature sensitivity of the produced composites, sintering could be carried out by conventional methods, and therefore, cold sintering was selected for the densification of the materials. The composites were consolidated up to ~90% relative density by applying a uniaxial pressure up to 1.5 GPa at room temperature for 10 min. Both the synthesised powders and cold-sintered samples were characterised from a physical and chemical point of view to demonstrate the effective production of biomimetic apatite/chitosan composites from mussel shells and exclude possible structural changes after sintering. Preliminary in vitro tests were also performed, which revealed a sustained release of strontium ranelate for about 19 days and no cytotoxicity towards human osteoblastic-like cells (MG63) exposed up to 72 h to the drug-containing composite extract.
Correction: Jamal et al. Preparation of 6-Mercaptopurine Loaded Liposomal Formulation for Enhanced Cytotoxic Response in Cancer Cells. Nanomaterials 2022, 12, 4029
Authors: Jamal Alam , Asseri Amer H. , Ali Ehab M. M. , El-Gowily Afnan H. , Khan Mohamed Imran , Hosawi Salman , Alsolami Reem , Ahmed Tarek A.
Published: February 2024

In the original publication [...]
Effects of Caffeinated Coffee on Cross-Country Cycling Performance in Recreational Cyclists
Authors: Trujillo-Colmena Daniel , Fernández-Sánchez Javier , Rodríguez-Castaño Adrián , Casado Arturo , Del Coso Juan
Published: February 2024

The ergogenic effects of acute caffeine intake on endurance cycling performance lasting ~1 h have been well documented in controlled laboratory studies. However, the potential benefits of caffeine supplementation in cycling disciplines such as cross-country/mountain biking have been rarely studied. In cross-country cycling, performance is dependent on endurance capacity, which may be enhanced by caffeine, but also on the technical ability of the cyclist to overcome the obstacles of the course. So, it is possible that the potential benefits of caffeine are not translated to cross-country cycling. The main objective of this study was to investigate the effects of acute caffeine intake, in the form of coffee, on endurance performance during a cross-country cycling time trial. Eleven recreational cross-country cyclists (mean ± SD: age: 22 ± 3 years; nine males and two females) participated in a single-blinded, randomised, counterbalanced and crossover experiment. After familiarisation with the cross-country course, participants completed two identical experimental trials after the ingestion of: (a) 3.00 mg/kg of caffeine in the form of soluble coffee or (b) 0.04 mg/kg of caffeine in the form of decaffeinated soluble coffee as a placebo. Drinks were ingested 60 min before performing a 13.90 km cross-country time trial over a course with eight sectors of varying technical difficulty. The time to complete the trial and the mean and the maximum speed were measured through Global Positioning System (GPS) technology. Heart rate was obtained through a heart rate monitor. At the end of the time trial, participants indicated their perceived level of fatigue using the traditional Borg scale. In comparison to the placebo, caffeine intake in the form of coffee significantly reduced the time to complete the trial by 4.93 ± 4.39% (43.20 ± 7.35 vs. 41.17 ± 6.18 min; p = 0.011; effect size [ES] = 0.300). Caffeine intake reduced the time to complete four out of eight sectors with different categories of technical difficulty (p ≤ 0.010; ES = 0.386 to 0.701). Mean heart rate was higher with caffeine (169 ± 6 vs. 162 ± 13 bpm; p = 0.046; ES = 0.788) but the rating of perceived exertion at the end of the trial was similar with caffeinated coffee than with the placebo (16 ± 1 vs. 16 ± 2 a.u.; p = 0.676; ES = 0.061). In conclusion, the intake of 3 mg/kg of caffeine delivered via soluble coffee reduced the time to complete a cross-country cycling trial in recreational cyclists. These results suggest that caffeine ingested as coffee may be an ergogenic substance for cross-country cycling.
Epigenetic Genome Modifications during Pregnancy: The Impact of Essential Nutritional Supplements on DNA Methylation
Authors: Socha Maciej W. , Flis Wojciech , Wartęga Mateusz
Published: February 2024

Pregnancy is an extremely stressful period in a pregnant woman’s life. Currently, women’s awareness of the proper course of pregnancy and its possible complications is constantly growing. Therefore, a significant percentage of women increasingly reach for various dietary supplements during gestation. Some of the most popular substances included in multi-ingredient supplements are folic acid and choline. Those substances are associated with positive effects on fetal intrauterine development and fewer possible pregnancy-associated complications. Recently, more and more attention has been paid to the impacts of specific environmental factors, such as diet, stress, physical activity, etc., on epigenetic modifications, understood as changes occurring in gene expression without the direct alteration of DNA sequences. Substances such as folic acid and choline may participate in epigenetic modifications by acting via a one-carbon cycle, leading to the methyl-group donor formation. Those nutrients may indirectly impact genome phenotype by influencing the process of DNA methylation. This review article presents the current state of knowledge on the use of folic acid and choline supplementation during pregnancy, taking into account their impacts on the maternal–fetal unit and possible pregnancy outcomes, and determining possible mechanisms of action, with particular emphasis on their possible impacts on epigenetic modifications.
Preventive Vitamin D Supplementation and Risk for COVID-19 Infection: A Systematic Review and Meta-Analysis
Authors: Sartini Marina , Del Puente Filippo , Oliva Martino , Carbone Alessio , Bobbio Nicoletta , Schinca Elisa , Giribone Luana , Cristina Maria Luisa
Published: February 2024

Over the past few decades, vitamin D has been found to play a crucial role in bone homeostasis, muscle function, oncogenesis, immune response and metabolism. In the context of the COVID-19 pandemic, numerous researchers have tried to determine the role vitamin D might play in the immune response to the virus. The aim of this systematic review and meta-analysis is to demonstrate that preventive vitamin D supplementation can play a protective role in the incidence of COVID-19, mortality and admission to intensive care units (ICUs). A comprehensive search on the PubMed/MEDLINE, Scopus, Cochrane and Google Scholar databases was performed on 15 May 2023, and two of the authors independently screened the literature. As effect measures, we calculated the Odds Ratios with their corresponding 95% confidence intervals (ICs). The assessment of potential bias and the evaluation of study quality will be conducted independently by two researchers. Sixteen publications were selected for inclusion in the meta-analysis. Our findings indicate that vitamin D supplementation has a protective effect against the incidence of COVID-19 in RCT studies (OR 0.403, 95% IC 0.218, 0.747), in the incidence of COVID-19 in analytical studies (OR = 0.592, 95% IC 0.476–0.736) and in ICU admission (OR 0.317, 95% IC 0.147–0.680). Subsequent analyses were conducted by type of subject treated (patient/healthcare workers) and type of supplementation (vitamin D vs. placebo/no treatment or high dose vs. low dose). Our meta-analysis suggests a definitive and significant association between the protective role of vitamin D and COVID-19 incidence and ICU admission.